LOCUS       DAOMYJ010000001       110945 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Prosthecobacter sp. isolate AMR_MDS_5629
            NODE_74_length_110945_cov_21.182451, whole genome shotgun sequence.
ACCESSION   DAOMYJ010000001 DAOMYJ010000000
VERSION     DAOMYJ010000001.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867850
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prosthecobacter sp. (wastewater metagenome)
  ORGANISM  Prosthecobacter sp.
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Verrucomicrobiaceae; Prosthecobacter.
REFERENCE   1  (bases 1 to 110945)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 110945)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.30
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:34:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,742
            CDSs (total)                      :: 4,684
            Genes (coding)                    :: 4,676
            CDSs (with protein)               :: 4,676
            Genes (RNA)                       :: 58
            tRNAs                             :: 55
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..110945
                     /organism="Prosthecobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_74_length_110945_cov_21.182451"
                     /isolate="AMR_MDS_5629"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1965333"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..224
                     /locus_tag="PLP58_00005"
     CDS             <1..224
                     /locus_tag="PLP58_00005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ06931.1"
                     /translation="KIAIEDTHATDLHPLAKKILTHFADHETLTLSQLVKLTHGKPST
                     LKLRLKELVQHGHLQPKGQGRGAHYVKRL"
     gene            255..1448
                     /locus_tag="PLP58_00010"
     CDS             255..1448
                     /locus_tag="PLP58_00010"
                     /inference="COORDINATES: protein motif:HMM:NF036995.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PDDEXK nuclease domain-containing protein"
                     /protein_id="HRJ06932.1"
                     /translation="MKKSRDTTLALTTDYKRFVTDLKSRIASARLAAARHVNRDLVTL
                     YWDIGRGIVEKQKELNWGESVVEMVAAELRAAFPDMSGFSPRNLRDMKRLYLAYSSEA
                     IWPQLVAKLENEGPKAKFLRQPVAEKASSTKWPQAVAKLGSTEPGSFLPQLVAEIPWG
                     HHRFLLDKVSAPAARLWYLRATASFGWSRNVLLNQIKAGAYERAVTEKKTHNFDLALP
                     EHFAEQAEEMLKSSYNLEFLGLRRAVRERELEDRLISRLQAFLLELGYGFCFVGRQHR
                     LVLGKKEYFIDLLFYHRFLKALVAFELKLGPFEPEFAGKMDFYLNLLNDTERAPGDQP
                     SIGIILCAEKDDVEVEYALRTKANPIGVAAYTLRSKLPGELKGRLPTAKQLADIVRSE
                     MEGGK"
     gene            1508..3103
                     /locus_tag="PLP58_00015"
     CDS             1508..3103
                     /locus_tag="PLP58_00015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ06933.1"
                     /translation="MRQIIQIIFCVLLVQNNALATTYILPTWEESSTTSEFVGVVECI
                     VAGGIVARYRVVDSWKGAAVGTELHIAQHVDPYGPQFPISLVGERSLVFAEKSPTYRL
                     GSFSGGGATPLWWRKIPTDLRCFSVVPIKEPMGRCLSIYMGFRGGGLAQFKAEVTSFL
                     GADSEGQEMRLMLAAARKNLHLPDPTEPSDKGNEEDIKLYEWLSGAKDIEQLWQRILA
                     QAKQLNPPPIPKTAEEIKADSHKRILLEMISSGGRERCKALLELADVSTLPWDKKELD
                     RAASYIRRSLSRAEGRIIPSQDSEQNKPPATAEQIAKAESALSKPWDGETGKAFELLC
                     HHSPSSLVPFLNGWQPTKDRRNQQFGYTLGSVFGHLCASDRIQHLSSLLAANDDWIKV
                     SGAVYLCFDDAKAGKEALSRLLSLQADAGAWAAIVLASRGEKAAMPRALEVMATPDDP
                     SIFSINHHNLQLRLRVLLSNVASASSISQPPGPSKEYSDGPERLIIQKEWHESLSKWW
                     EMNQDKIKLADPWAKFLDEQRVD"
     gene            3215..4636
                     /locus_tag="PLP58_00020"
     CDS             3215..4636
                     /locus_tag="PLP58_00020"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase"
                     /protein_id="HRJ06934.1"
                     /translation="MKSVLTLFAISAFFGGHALAAESRPNMLFIALDDLNDWIGCLGG
                     HPQTRTPNLDRLAASGVLFRNAYTAAASCNPSRTAIFSGLAPHRSGLYHNLQKMREVM
                     PEAELLPRYFSRQGYWSAGSGKMLHYIIDPPSWDDYFPARAKDNPFPHTFYPEKRPVN
                     LPRAGDWQYVETDWAALDVTDEQFGGDWAVTKWIGEQLQRPHDKPFFLACGIYRPHEP
                     WFVPKKYFEPFPLESIQLPPGYKADDVDDLPPAGQRLARNRYFAHIQKHEQWKKGIQA
                     YLASIHFADAMLGRVLDALEESPQRDNTIVVLWSDHGWHLGEKEHWQKFTGWRVCARV
                     PLMIRVPKGAPGLPEGTTPGSVCDRPVSLVDLFGTLTDLCGLPPKADIESRSLAPLLR
                     NPQADWPHAALTHLDKPENYALSTQRWRYIHYTGDEEELYDLETDPHEWTNLATKPEH
                     ANKLAEMRLLAPKNIAPVHKSKP"
     gene            4633..5835
                     /locus_tag="PLP58_00025"
     CDS             4633..5835
                     /locus_tag="PLP58_00025"
                     /inference="COORDINATES: protein motif:HMM:NF015585.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialate O-acetylesterase"
                     /protein_id="HRJ06935.1"
                     /translation="MKAIVLTSLLMAVHLHAKPLQVFILAGQSNMQGHAKVSTFEHIG
                     MDPATKPMLDEMMGADGKSTICERVWISSIGCAGDNTTEQTGKLTAGFGATKEKIGPE
                     LTFGLTMEKLIDAPILLIKTSWGGKSINTDFRPPSAGPYVFNEAQLATMQKQGKDIAA
                     LKAAKEKEAGVYYRLMIEHVKKVLADIKRVVPSYDASQGYELAGFAWFQGWNDMVDNG
                     TYPTRDKPGGYDDYSKVMAHFIRDVRKDLDTPKLPFVIGVMGVGGPTSEYGPNEQRYK
                     ATHQNFRDAMAAPAELPEFKGNVVAIRTEKYWDRELKLARAKENEVKDKAKKLAKEQK
                     LKPAEERALADKMRADSLTERERLVLEKGVSNAEFHYLGSAKILGGIGKGLAEAMAEL
                     NHLKNTVR"
     gene            5832..7187
                     /locus_tag="PLP58_00030"
     CDS             5832..7187
                     /locus_tag="PLP58_00030"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase"
                     /protein_id="HRJ06936.1"
                     /translation="MRIAALILFALAGLCSANAKPNIVVIMADDLGWRDLHCYGNASV
                     DTPTLDQLAADGMRFTDAYAASPVCTPTRAAMMTGQSPARLRITNHAPGNPDGFALEG
                     SSLHEAPNIRHLPLAAVTIAERLSAAGYATAHVGKWHLSHVVRGDKRLAETDLRPEKQ
                     GFDLNAGGNSSGGPASYFAPYRNSAFKDGQDGEYLPDRLADEAIRFIRQHKEQSFYLN
                     WWPYSVHYPMQAREALIAKYRQRTEIKDPIYAAMIEGLDTAIGSFLKALDEAGLRENT
                     LVLFKSDNGGYHGDNRPLRGFKGMLYEGGIRIPWIVRWPGQVPPGTLNSTPVISTDCY
                     PTLLEVAGLPPTEAHPVDGKSLLPLLTQTSGFQRETLYFHYPNYAFHKRNRLGSALRE
                     GRYKLIQNYDDHSLELYDLGSDIGEKNNLAAQAPDLTRRLAEKLQAWLVKSEAQLPAR
                     H"
     gene            7200..9617
                     /locus_tag="PLP58_00035"
     CDS             7200..9617
                     /locus_tag="PLP58_00035"
                     /inference="COORDINATES: protein motif:HMM:NF039401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6288 domain-containing protein"
                     /protein_id="HRJ06937.1"
                     /translation="MKLISSFTLALSVLFAWPALAAKPGETLTPPDFTKGGRPDEAHD
                     WTLGPTGARGWIFTSRGHSRDARQILVTKIAPGSPADGVLAVGDVILGVNAEPFADDA
                     RVLFAKAITAAEAETGALRLLRWRAGKTDTVELKLAVLGGYSANAPYDCAKSGRIFEL
                     GCASLAKRMADEKNYTKQLNPIPRALNALALLASGDKENLPLVKREAQWAADFTTDGY
                     LSWYYGFLMMFVSEYVMATGDTSVMPGLTRLALETARGQSGVGTWGHRFATSGGNLSG
                     YGAMNAPGLPLTIGMVLAREAGVSHPDLDRAITKAAGFLRWFVHKGAVPYGDHLPFPA
                     HEDNGKCAAAAVLFDLLGDREAAEFFAKMAAAAYSERERGHTGNYFNILWAMQGVARC
                     GSLTSGAYWQEQSWYYDLVRTWDTAWVYQGSPVGAEEHGAYKGWDTSGSHLLDYALPL
                     KSLRLTGRKAFTMPPLNAAQTAAVIAAGRDFAFKDDANRYEQRTSEQLLAGLASWSPF
                     VRKRSAESLGKRQGDFVPALLKLLASTDRDTRYGAVEALGALGQRADAAAPQLRAALK
                     DTDPWLQSLAAEAIPTLGPAARKTSVSDLLAMTVRTNPADPRRLAARSASVALFANYP
                     GQRGPRSILADSLDGVDRAQLYPAIRALLQHEDSIPRGSVSKALDRLTDADLVELLPD
                     IAKAIEHIAPSNEMFGDGIRLAGLDLLSRLHIREGMALCLSVMEPERWGEKNRSHACL
                     TALQRYGTHAKAMLPKLQVVREQLVTARRSKEHLAEFDKAVAAIQSSTETPTLVDLRA
                     FQARAVN"
     gene            9627..11198
                     /locus_tag="PLP58_00040"
     CDS             9627..11198
                     /locus_tag="PLP58_00040"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: protein motif:HMM:NF015284.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent,
                     subunit alpha"
                     /protein_id="HRJ06938.1"
                     /translation="MTPVSIFNDVIGPVMRGPSSSHCAAALRIGRLALDLMDGEITEV
                     LVEFDRAGSLPTTHESQGSDMGLFGGLLGWNADDERLPNSAQALADAGIKLHIETVDV
                     GDPHPNTYRLTLKNPRETHTLIAISTGGGMIEVIEIDGVPLCMDGGYHETLIWLSKCD
                     ADTLVCILGFSEADKSVCITHDLGDTQLIQIKSSQFAATSDLKPISVKKLSPVLPVPS
                     RKDLHVPFTSCAAMLQHDGDQNTPLWQLAIEYERARGNFTKREVIDKMTGIVRILRKS
                     IASGIAGTSYEDRVLHHQCGRFAEMMNTGRLLDGGALNRIILYVTALMEVKSSMGVIV
                     AAPTAGACAALPAAVIAMAEAMNQDEEQMAKAMLAAGMIGTFIATRWTFAAEVGGCQA
                     EGGAAASMAAAALVTHAGGTRDQAIAAASMAFQSMLGLICDPIANRVEAPCLGKNVMA
                     ASNALSCANMALAGYDPLIPLDEVIDAAKAVAERMPREHRCTSLGGLAVTPTSLEIEK
                     RLASFKKGCGTCACH"
     gene            11362..12792
                     /locus_tag="PLP58_00045"
     CDS             11362..12792
                     /locus_tag="PLP58_00045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009958599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arylsulfatase"
                     /protein_id="HRJ06939.1"
                     /translation="MLRLFSLIVPAFCLLAGFATSGFAKDQPNIIYFLADDLGWTDVG
                     WHGSEIRTPNLDKLANGGAKLEQFYVLPVCSPTRAALMTGRYPIRHGLQVSVVRPWAQ
                     YGLPLEERTLPQALKEAGYTTHISGKWHLGHFLPDYLPTRRGFDTQYGHYNGALDYFT
                     HDRDGGHDWHRNDKDSRDEGYTTNLIGDEAVRVVEQAQPGKPFFLYVPFNAPHSPLQA
                     PDEYLKRHAHLADRKRQAFAAMVECLDEQVGRVVAALEKRGLVKDTLILFSSDNGGPL
                     GLGATNGSLRAQKGTLYEGGTRVCAFANWAGRIKAGTVVNEPLHIVDWFPTLVKLTGA
                     PAAQKTPLDGLDIWPVITAGKPSPHEDILINATPGSGAIRMGEWKLVLNGRIDANDLQ
                     NAGPPNAEAKAAPKKKKAQEKAAGVAVELFKLADDPYERNNLAGQHPEVVQQLRARIE
                     AYQKAAVPPKAGNQPANYKAPRVWGE"
     gene            13046..15796
                     /locus_tag="PLP58_00050"
     CDS             13046..15796
                     /locus_tag="PLP58_00050"
                     /inference="COORDINATES: protein motif:HMM:NF019223.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PSD1 and planctomycete cytochrome C
                     domain-containing protein"
                     /protein_id="HRJ06940.1"
                     /translation="MRFPTFLLFVTSASAAPVDFVRDVRPIFERHCLECHGEKKQKSG
                     LRLDIKSEAFKGGDNHGPDIVPGKAKDSPLIRFLTTDDDDELMPPKGRLPAVDIETIT
                     NWINEGAGWPEGADLAKLEDRLDHWSFKPLLEFNPRHTIDAFIDAKLSKKGLHRSPPA
                     DPVTWLRRVTFDLTGLPPTPEDVIAFESDLSDSSDQSDAYEAVVARLLNSPRYGERWA
                     QHWLDVVRYADTHGFEVNTERPNAWPYRDYIIRAFNNDTPYDRFIQEQIVGDAMGEDA
                     ATGFLVTASVLLPGQIGKDEPSKRLARQDSIDEIVVNIGQTFLGLSIGCARCHDHKFD
                     PISAKDYYSMQAFVAGVEYGERELRTTEAIAAREELKKVKAQLTDIETQLARFVPLAK
                     SGVKRPMIDARENRDRFMPVKARRVRFTIRDTNKLEPCIDELEVFTTAGENIALNAPI
                     TVSGEKVSADRHEQRFVNDGNYGNSRSWMSNEVGKGWVMIELPREQEIDRVVWGRDRQ
                     GKFKDRLATSYVIETADNSGMWRVVADSDDRQRFDERDRTQPAFSTQGLRTEEIAEAQ
                     SLMDQKRPLDERLQRLSADQKVFAGTFRTPDDIHLLNRGDPEQPQGSVVPAVLSALGK
                     VTLTRETAEQERRRALAEWITSPQNPLTARVMVNRIWQGHFGTGLVETPSDFGRMGIK
                     PTHPELLDWLAAEFIKSGWSVKHMHRIILMSETYRQSSVVSDQSDSSDALAKDANARL
                     LWRFPSRRLEAESIRDSMLAVSGLLDLKMHGRGYNLFDKRGGLSGFEPVETLTPDNKR
                     RMIYAHKVRREPEAVFGAFDCPDAGQSTAIRRASTTPIQALNLFNSRFTLDTANAFAA
                     RVQKEVGGETAAQITQAYQLALNRVPTAAELQGSEPAVRENGLSVLCRALFNSNEFLF
                     LP"
     gene            15793..17235
                     /locus_tag="PLP58_00055"
     CDS             15793..17235
                     /locus_tag="PLP58_00055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009964656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1501 domain-containing protein"
                     /protein_id="HRJ06941.1"
                     /translation="MSESLTPAGHHLLNRRQFLANSATALGSIALTRLLAADGLLIDP
                     ARPYAPRQPHFPAKAKNVVVIFCAGAVSQLETWDYKPELIKWDDKPLPGGPAVTFQGP
                     AGNLARPQYAFRPRGQTGKWVSNMIPHLAELTDDIAFVHSLTSKSNTHGPAENLLSTG
                     FTLDGFPSLGSWVTYALGSENQNLPSYVAIPDPRGVPQNGANNWGPGFLPAAFQGTAM
                     SSKSPVRHLSAPGVSAQADQAARSLLQKMNARHLEANPGDQKLAARIASYELAARMQL
                     SVPEINDLSTEPAHILKMYGADDTQNADKAAFAKNCILARRLIERGVRCVQLFNGAYA
                     SGGKLNWDGHSDLKSQYDVHASILDQPAAALIRDLKQRGLLQDTLVVWCTEFGRMPFF
                     QKGAKGRDHNPDGFTCWMTGAGVKPGVSHGECDELGQKAVKDIHPLYDFNATILHLLG
                     LDHELLTFEHNGVRRRLTNVEGRVIKEMVV"
     gene            complement(17242..18144)
                     /locus_tag="PLP58_00060"
     CDS             complement(17242..18144)
                     /locus_tag="PLP58_00060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ06942.1"
                     /translation="MRATRLADLKAKFATKSQAKFYVSRLGGDFGDYEREDAAYQQAI
                     SEAQRTLGELGRVQLVQRAFLPNFVFGEQDIVVALGQDGLVANTLKYLNGQPLVGVNP
                     DPKRYDGQLLPFQVPQLDKVMREVLRGGRSKREVTMAEAKLNTGLSLCAVNDLFIGMK
                     SHGSARYRIALGNTAENHSSSGVIVSTGLGSTGWFKSLMAGSMAVAVASGRAAATEPK
                     DTSFPWESNHLFFTVREPFPSNTTAAELVFGKVTPQQPLVIESLMGENGVIFSDGIEQ
                     DFIEFNSGTKATISISKRKGQLVA"
     gene            complement(18196..19221)
                     /locus_tag="PLP58_00065"
     CDS             complement(18196..19221)
                     /locus_tag="PLP58_00065"
                     /inference="COORDINATES: protein motif:HMM:NF013324.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HRJ06943.1"
                     /translation="MFGLRFIKIPPTTHVIQHSGGKVVREGAGISFFYYSPTTSLVAV
                     PVGSTDVPFIFEEQTADYQSVSLQGQITYRIADPKKLAALMDFTLAPHGRGYASEDPE
                     KLPQRVINLVHVLARAEIEKLPLRQALRASETLVTAIRAQLGTSAELAALGLEVLGLS
                     LLAIKPTPETARALEAETREQLLKGADEAIFMRRNAAVEQERAIKENELNTEIAVENK
                     KRQIRETQMDTEKAVQEKEAQLERAKLESSIGLEDERKKLVSLAAENAKAESDTRAYG
                     IEATMKALASTDASVLQALATSGMRPQQLIAFAFQELAAKADKIGNLNISPELLSELL
                     ANPGGKK"
     gene            complement(19253..19927)
                     /locus_tag="PLP58_00070"
     CDS             complement(19253..19927)
                     /locus_tag="PLP58_00070"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF015692.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribosylglycohydrolase family protein"
                     /protein_id="HRJ06944.1"
                     /translation="MKSAGNGAAMRGAIVGVIFRDDAAKRHEFALAACRVTHTDPRAE
                     ESALLVAEAAALAAHHAETKTVLDTLRPLVTSEEMKSRFAAMETGLSEKISVSDYAAR
                     IGCEHGVSGFAPNSVAAALFGWLRHRGDFERVLTELIVCGGDTDSVAAIAGGICGTET
                     GESGIPKAWITGIRDWPRSVTFIRSMAQESVAPPRVLWPAMLLRNLFFLGVVLTHGFC
                     RLLPPY"
     gene            complement(19924..20298)
                     /locus_tag="PLP58_00075"
     CDS             complement(19924..20298)
                     /locus_tag="PLP58_00075"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF015692.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribosylglycohydrolase family protein"
                     /protein_id="HRJ06945.1"
                     /translation="MSGENSTMNPSPASPVLGCLLGTAVGDTLGLPLEGLTRRHIQRW
                     TGGELRHRLIFGCGMLSDDTDHALMLAAALRRHCDDAAAMQRDFGRQLRWWLAALPAG
                     VGLSTARAILRLWTVFQPPDPA"
     gene            complement(20295..20810)
                     /locus_tag="PLP58_00080"
     CDS             complement(20295..20810)
                     /locus_tag="PLP58_00080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009962264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetyl-ADP-ribose deacetylase"
                     /protein_id="HRJ06946.1"
                     /translation="MPAELQALQGDITKLDVDAIVNAANTSLMGGGGVDGAIHRAAGP
                     ELVRECMKLNGCLTGEAKITKGYKLKARHVIHTVGPVWHGGGNREFDLLRACYTNSLK
                     LAAEHRLQSIAFPCISTGVYRFPADEAAKIAFEAVTEFLKEPSSVQSVAFCCFSREDH
                     DRYTALMDAKR"
     gene            complement(20838..21761)
                     /locus_tag="PLP58_00085"
     CDS             complement(20838..21761)
                     /locus_tag="PLP58_00085"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF015692.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribosylglycohydrolase family protein"
                     /protein_id="HRJ06947.1"
                     /translation="MTSSTHAERLERTRLSLDGLSVGDAFGQMLSSCARSARNVVEPG
                     GLPSGPWWHTDDTQMAMAIVEELVAHGQIATDSLARRFAERYQADPGRGYGKGARMQL
                     EEIAAGESWRTTAAAAFNGRGSKGNGSAMRVAPLGAWFADDPAQIVSQSTLSSVVTHS
                     HPEGVAGSIAVSLAAAGAWASRILPLEKAREWIWDAVLGQTPAGETLEGIRKARLVPL
                     EMSAENAARILGSGFLVTAPDTVPFAIWCAIRHLDCYSEALLATLEGDGDCDANCAIV
                     GGIVSLRVGRDGITADWLASREPLDLRLRTH"
     gene            complement(22177..22656)
                     /locus_tag="PLP58_00090"
     CDS             complement(22177..22656)
                     /locus_tag="PLP58_00090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="NADAR family protein"
                     /protein_id="HRJ06948.1"
                     /translation="MKTKLPISRDDLVLAMHAGLRPKWAFFWGHTSAKDGSITKACFS
                     QWWSRHAFEVDGIRYPSAEHFMMAEKARLFRDDVHLPAILAAKSPAIAKKLGRQVTGF
                     DDNLAPTSLGHRRTRQRSQVHPAPGTKTVSPANWRPNHRRSQPLRSHLGHRHVRRSS"
     gene            complement(22653..23336)
                     /locus_tag="PLP58_00095"
     CDS             complement(22653..23336)
                     /locus_tag="PLP58_00095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009963479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HRJ06949.1"
                     /translation="MPHTYQYPRAALTVDCVVFGFDGSELQVLLIRRALEPFRDRWAL
                     PGGFVRVEETLDEAARRELEEETGLRDVFLEQLYTFGTVKRDPRERVVSVAWFALVKP
                     SVVTADTDAAEAKWFPVNDVPPLAFDHADILATALTRLRGKLTYQPVGFELLPPKFTL
                     TQLQRLYEAVLGEELDKRNFRKKALSHDLLIPLKEKHQEGAHRPAQLFRFDPVKYERL
                     KKKGFLFEI"
     gene            23491..23601
                     /locus_tag="PLP58_00100"
     CDS             23491..23601
                     /locus_tag="PLP58_00100"
                     /inference="COORDINATES: protein motif:HMM:NF019040.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1501 domain-containing protein"
                     /protein_id="HRJ06950.1"
                     /translation="MLHLLGLDHERLTFEHNGVQRRLTNVEGHVIKEMLA"
     gene            complement(23627..24778)
                     /locus_tag="PLP58_00105"
     CDS             complement(23627..24778)
                     /locus_tag="PLP58_00105"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialidase family protein"
                     /protein_id="HRJ06951.1"
                     /translation="MILCALPASAAEPWFETTCLFPVTPANKPNYRIPALIQAPNGDL
                     LVVCEKRNDGPGDIGDHDIVMKRSSDGGKTWTGEILILDDEDRVCTDLTLGFDRGTQK
                     LWLFFLKDKKQFAHLSSTDNGLTWQGPVVIHDQVTKPEWDQLRGKPDTKPFNPKSHAQ
                     RWERDWHQRYGCGPGHAMVQLKSGRLLVPARHREDIGGGRLRSFAHCFYSDDHGASWQ
                     LGGTIGMHTSECQFIELNDGGIRVISRNESAEDAPDNLRHLTSISKDGGQTWSALRRQ
                     EELITPRCHGSLERLDEKTLLFCSPASPFRQKEHPYGRYNLTIRISHDEGASWSAGRA
                     VWPHPGSYSDIAVMTDGSIAVVYERADKGSSHYWDELHFARFNPDWITQ"
     gene            24983..25927
                     /locus_tag="PLP58_00110"
     CDS             24983..25927
                     /locus_tag="PLP58_00110"
                     /EC_number="2.7.1.15"
                     /inference="COORDINATES: protein motif:HMM:NF012515.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019200 - carbohydrate kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HRJ06952.1"
                     /translation="MARAAPANLVVVGSMNVDLIASVKRLPAPGETVSASALEKRFGG
                     KGANQALAAARQGAAVTLIGCLGDDPDGRDYRNHLRREGVNCSSVNTTRGGTGSAFIA
                     VDAKGENQIIVIPGANSALTAPSVKVQRSRIEVAKTLLLQLEVPMESVIEAIRLANGR
                     QVPVVFNPSPCRADFPWAKVRVEVLIVNAGEARMIFDADPAGMQDGVAAWQEKLKEKN
                     IGILIVTRGEKPTLVLHSEGFKKVATHPVKPVDTVGAGDTFAGAFSAFFAEGMPLLEC
                     VRWANAAAALATLKHGAQDGIPMRGDVQSALSHPEGKK"
     gene            26003..26869
                     /locus_tag="PLP58_00115"
     CDS             26003..26869
                     /locus_tag="PLP58_00115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009960094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase"
                     /protein_id="HRJ06953.1"
                     /translation="MTTVTLGLIQGGTHAGKAESHARHERLIREAAARGARIICLQEL
                     FDIPYFCTRQDTALFDLAEAVPGPTTEKMSALAKELGVVLIVPLFEKRGPGLYHNTAA
                     VLDADGAYLGKYRKMHIPQDPGFEEKFYFTQGDLGYRVWDTKFGRIAVLICWDQWFPE
                     AARLAALAGAQILFYPTAIGWLASEKAALGKAQHCAWETVQRGHAVANGCFVAAVNRT
                     GFQDETEFWGQSFVANPYGEIVAKASVSEQEALVVPCDFQVVEDFRRIWPFFRDRRID
                     TYAPLTKRYLDE"
     gene            26862..27908
                     /locus_tag="PLP58_00120"
     CDS             26862..27908
                     /locus_tag="PLP58_00120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009960095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="agmatine deiminase family protein"
                     /protein_id="HRJ06954.1"
                     /translation="MSKGYPKNHRLPAEFEPQEAVWLSWPSNKESCPKTYLKLQDKFG
                     EIAANMTRYQRVRINAPENMHMGIRLSIADNEGALNMVDLYNHQTNDVWCRDHGPIFI
                     KNDTTGQVSITDWKFDGWGGKFEAGLDNTIPEKAAEVLKMDRYVSDMVLEGGAIETNG
                     KGLLFTTEAVLLNPNRNGGKARKKEEVEKELKAMLGVTDIVWFKKGIEGDDTDGHIDD
                     LVRFVREDAVICMVESKENDPNYKALKQIREQLDDVRTADGSKLEIIEIEMPNAIEAR
                     DWRLSRLPASYANFLIVNNAVFVPIFGQKKKDAMAEDKIAECFPGREIISVTCKDLVM
                     EGGALHCIAMHQPK"
     gene            27992..29293
                     /locus_tag="PLP58_00125"
     CDS             27992..29293
                     /locus_tag="PLP58_00125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009958568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003979 - UDP-glucose 6-dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide sugar dehydrogenase"
                     /protein_id="HRJ06955.1"
                     /translation="MNVSIFGLGYVGAVTAGCLAELGHGIVGADVQQAKVDAFNTGRS
                     PIIEPELDDLLGTARAGGRLRATTSAEEAVAATDASIICVGTPSLESGRLNLDFVRKV
                     SEQIAAALRVSGKRHVILFRSTMLPGSTRQMVRDFFEDLRVSGQVSVYYCPEFLREGT
                     AVRDFREPSLAVVGTHDGREPESDEARRLLGGTPAVLAWEGAEMIKYACNYFHALKVG
                     FANEIGRLSKFLGEDGARVMDVVCADTRLNISRYYMKPGNPFGGSCLPKDVSALLSFA
                     RQEGITLPLLDNTLDTNQAHLDLLIKLITSKGARRVGLLGLAFKADTDDLRGSPMVAV
                     AETLLGRGFHLSIYDPSLNLSRLIGANEAEIQRRMPHLASLLKDDPREVLESSDLIVA
                     SQKCVPLEQLATLVNANQYVIDVNGWRELAELPWSYEGLCW"
     gene            complement(29303..29920)
                     /locus_tag="PLP58_00130"
     CDS             complement(29303..29920)
                     /locus_tag="PLP58_00130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ06956.1"
                     /translation="MSTIVIAQKNGVACIGADTMSCLGSLRQRAHHVVNKTKITKIGD
                     TFIGLTGTSASLVVMNSYFANPERPRDFSSTDMIFETFRHAHHWMKTEYFMSTMASKE
                     EEYETTQFYGVLANPHGIFALYSYRSAQQFHKFWAAGSGRDFALGAMQMVYDPFDNVV
                     DIVRAGLNAAAEFDSATGAPYEIHTCALNSAPQAARKPKKRAKAR"
     gene            30041..30670
                     /locus_tag="PLP58_00135"
     CDS             30041..30670
                     /locus_tag="PLP58_00135"
                     /inference="COORDINATES: protein motif:HMM:NF012264.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HRJ06957.1"
                     /translation="MTFLRLPILLTASLTLPLTAQEGPLLFQTVCAQCHGPEGGGRDE
                     LKAPSIAALPAWYVTTQLANFHDGKRGRDGAADPQGAMMAAVAKALSETQIQALAAHV
                     ESLKEVAPAVLVVEGADVRAGQELFYERCMECHRYNASGEMVFGSPPLTGRQGWYLLA
                     QLQKFKGLHRGAAKGDEKGAKMVEMATKFIEDEQTMKNLVGYILTLNKP"
     gene            30702..31220
                     /locus_tag="PLP58_00140"
     CDS             30702..31220
                     /locus_tag="PLP58_00140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007415635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase family protein"
                     /protein_id="HRJ06958.1"
                     /translation="MRIITCGPEHLESIRAIFNEAIVNSTAIYDYHPRSVETLRGWFE
                     AKQARGLPVIGVENGDGVLMGFGSYGPFRALPAYKYTVEHSVYVDARFRGLGLGRLLL
                     EQVIQSAQARGFHMLVGVIDAENAASIALHERLGFKSGGGLRHAGFKFGRWLDLVFYQ
                     KLLPTPESPVDG"
     gene            complement(31178..32263)
                     /locus_tag="PLP58_00145"
     CDS             complement(31178..32263)
                     /locus_tag="PLP58_00145"
                     /EC_number="1.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009963567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004152 - dihydroorotate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006222 - UMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quinone-dependent dihydroorotate dehydrogenase"
                     /protein_id="HRJ06959.1"
                     /translation="MSLAAQLYPLLKPWLFRMDAERAHEWTTRMMRLSHACGLLALGA
                     DETRRKADVISCMGLAFPNRLGLAAGMDKSASAVDAWAALGFGFVEVGTLTPRPQPGN
                     PKPRLFRLPEHEALINRMGFNNPGIEAAVRRLRRRKTRAVVGVNIGKNFDTPNEEAVR
                     DYLACLKAAYPVANYIAVNISSPNTKGLRDLQAEDSIRALLAALKSEQERLAGEHGEK
                     KPLLVKIAPDLDGQQIEALARVFNELAVDGVIATNTTIGREAVAGHPLAGEAGGLSGA
                     PVRERSTLVVQAFRMLLRKGTPVIGVGGILSGADALEKHSAGADLVQLYSGLVYRGPG
                     LITEALEALRGASSHPSTGDSGVGSSF"
     gene            complement(32320..33399)
                     /locus_tag="PLP58_00150"
     CDS             complement(32320..33399)
                     /locus_tag="PLP58_00150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007413687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HRJ06960.1"
                     /translation="MPTPNPRYDVLSLGEVLLRLDPGEGRVRTSRQFTAWEGGGEYNV
                     ARGARRCFGLNTGILTAFADNEIGRLLEDLILQGGVDTSLIRWSPYDGMGRRVRNPLN
                     FTERGFGARGARGSVDRGHSAASQIKPGDFDFDHLFGVLGCRWLHTGGIFAGLSESTA
                     LVTIEACEAAKRHGVTVSYDLNYRPSLWQERGGFPAAQALNRRLAPFVDVMIGVLCDE
                     PPAAGPLIPGSADLFAAEDATLRPAMEKLAAEFPNLQTIAATLRRVHTASVNDWGALC
                     WHKGSFYRSRDYPRFDILDRIGAGDSFVAGLIAGLLRENDPQKAVDYGAAHGALAMTT
                     PGDTSMATLPEVEKLASGGDAKVQR"
     gene            complement(33470..33841)
                     /locus_tag="PLP58_00155"
     CDS             complement(33470..33841)
                     /locus_tag="PLP58_00155"
                     /inference="COORDINATES: protein motif:HMM:NF014655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease adaptor ClpS"
                     /protein_id="HRJ06961.1"
                     /translation="MIPRPQQTVTNATDTPVLPARPRQMPAQPKMQPELEPPWHVILH
                     DDDTHTYEYVIEMMVEIFGYDEARGFKIACEVDESGRVIVATCHKELAELRVEQIHDY
                     GADPRMKQSTGSMKATMEPAE"
     gene            complement(34006..36504)
                     /locus_tag="PLP58_00160"
     CDS             complement(34006..36504)
                     /locus_tag="PLP58_00160"
                     /inference="COORDINATES: protein motif:HMM:NF015131.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c peroxidase"
                     /protein_id="HRJ06962.1"
                     /translation="MTGPRAAVTDMTNPLGLAGSAFGSLAARVIRDSLHSYWHGGQSA
                     AQSSHDKTTKEQAKTPPPEPPGIFARRSKPAPPGEDVSWLDKQIVRLAELEAGRTKRN
                     SPFPTSDAHRRFLNASASWRLRIAYELDPGDPVLYEILHYQLAARLAEKPETRQKLDA
                     LAERAITHGTAPMSGLNAALAGAGAALNLLNDLLRPGQPKRDDDAIIRRWGQLDRCLA
                     RYRAIRHDAVESGWWSGIPEVRQQELEQHAALLGRIAEKIRLQLPAARLTVQIQHRWH
                     GAPLPLAKPGLLNAAGDKLSVTRLAYLLSKAMLRTESGEWISAGDWFAFLDVEKDRTH
                     FTLPRVPRGRYTALRFDLGLDEQTDKSDPARWPAGHPLNPDLNALHWSWKDGYVFLAI
                     EGHYERPGENPGGYSYHLAGQDCAGTIEVPVEMDLNGDLRLTLAFDTERVFAAAHTIR
                     IPDSDSTHSHDDGGLAARMADNAVQAFALLGIQPDLQNTGPAELVGSVNAPPTLAGHV
                     PAHFPLTTWPEDNTPTPDGVALGRRLFEDKRLSINNTQSCSSCHHQQAAFAEPLATST
                     GAQGQTGARNAMPLFNLAWKPSFFWDGRSPTLRDQVLRPIQDPLEMNETLPGVIAKLR
                     ADPGTLGDFEKAFGTREITPERIALALEQFLLTFISGQSRLDLARAGKAELTEQEQHG
                     FRLFFTESDPARGIRGADCFHCHGGAHFTNNQFHNNGLDPDDLIRDIGREKVTGKPGD
                     KGKFMTPSLRNAALTAPYMHDGRFKTLEEVVDHYDRGIKSSSTLDPNLAKHLTHGGLG
                     LTPEEKAALVAFLKTLTDTSFTEKPSVMAGVRTQ"
     gene            complement(36546..37316)
                     /locus_tag="PLP58_00165"
     CDS             complement(36546..37316)
                     /locus_tag="PLP58_00165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ06963.1"
                     /translation="MKSLPLLRMSLAGLMSRSSCWLLLGGALVFAWLAPLVTPWQENP
                     QILQPARAQAAWVFAWLALFTWLPFQAAALGRRLRVEGMLEHLRSGGRRAWNLWLQLG
                     SAVLLWMLAVAVIAALVCVVFCLPQRAGEGALWTQLVLQYTALYFICAAPLVLLAAAL
                     GTRTAEVIAFLVPVALLFTGLFGSAWLAPMLGGSSSSLIQTLWLAVPHYHLADLTPRL
                     VFKMGPLPSADFLATLGGLTLQGAALSLLGLCAFRTRS"
     gene            complement(37313..37993)
                     /locus_tag="PLP58_00170"
     CDS             complement(37313..37993)
                     /locus_tag="PLP58_00170"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HRJ06964.1"
                     /translation="MSEQPALTIPAGTRFGYREGPLWHRRQRCLVETPRDVVTGPGLH
                     LLVAPNGSGKTTLLRTLAGLLPALSGSVNAGGHVHYFSDELRADSELKPRTLFRSLLK
                     NGALAMAEELAETLRLDLNRPIGQLSRGNRQKVLLILAEVKVAHENAGVLLMDEPLTG
                     LDAETRAQMTALWSSAQTKAVRLVILHELESVRHADSLLSIHGGSLAHAREKSGATWL
                     DTYHSLQS"
     gene            complement(38017..38427)
                     /locus_tag="PLP58_00175"
     CDS             complement(38017..38427)
                     /locus_tag="PLP58_00175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HRJ06965.1"
                     /translation="MNPRPTKTDSTRRGYSLLELSLVMALLLGLSTFAGLTVSSIRDW
                     QRGKDASLSLQAVFAAQRAWMADHPTGDIELVSAAQLQTYLPEGWYAMPAFVSLSGES
                     LTLDYNVMPPLLYTSGVVYDPSGAPDDGLWDVGK"
     gene            complement(38402..38812)
                     /locus_tag="PLP58_00180"
     CDS             complement(38402..38812)
                     /locus_tag="PLP58_00180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ06966.1"
                     /translation="MHILILALAASAAWADPTPRLVPRPIPGMVHAPRPASTQVVRRD
                     GAPAKAAGASQGVPSVEAARIVFIHRAATLPPELRLVEKPRCPEPAATAIKPVIQERP
                     RKMRIIPQSEAAALAALAEKPAALTAHEPSPDKD"
     gene            complement(38831..40048)
                     /locus_tag="PLP58_00185"
     CDS             complement(38831..40048)
                     /locus_tag="PLP58_00185"
                     /inference="COORDINATES: protein motif:HMM:NF012694.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HRJ06967.1"
                     /translation="MNAFQVTLRHSERPDLSRVLEIEAPNREQAAALAAKPGFRVALV
                     KPLKGVKKSGRAKGGIPRKELIKLFRGLASMLKANISTSDALLYYAQGLPDPALQAAL
                     MNIRNRLEAGIPVQAAFAKEQCFDSTIITIIEAGSDSGQLGDAFSSLARRIKIEMMFA
                     SKLRSALLVPSIIILFQIGLFIYSQVYVVAQVEDTLKSVRQEPDALSQVIFSISHFVQ
                     KVWPFFVIALTSFLVSIFRSAPLRQRLLIFLMGRWTLLKKLVMGLRQSAWIGTLQMLY
                     ANGINLARASALSARVLAGTPMYEDFIKASTTYESSGVPFAESLKRHTELDPQVIHMI
                     GIGERSASLPQQLELLRDIYEEDTAAIMSDFTQVINFVTLAMAVVLIGFVFTGSMLPI
                     FLMGPRMMQSGNM"
     gene            complement(40052..41743)
                     /locus_tag="PLP58_00190"
     CDS             complement(40052..41743)
                     /locus_tag="PLP58_00190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009958486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase, T2SS/T4P/T4SS family"
                     /protein_id="HRJ06968.1"
                     /translation="MNLNLGIEFNDTIQRLIVGQLRSSMAAQEGVSDEALVRKSSKTR
                     ILGAVGRASGLPAFPQVRDLVDQEFVGYCDPAVLSRGMFVPLRRSPKQILLAVANPWD
                     YRADDYCAARFPDDEVLKVVTLASEVSSVIEGSASSSGPSRAELEAVEVEEFSNECPD
                     FDVTRQHEEPIAQLVSSMVSDAIKQHASDIHIKTEKTLFQYCFRIDGDLGARVDMPMK
                     LRDRVDAFMLNLMRLAPEERNKRPGISGRFTASYYGRAVGVRYERHRTYRGYHVTMRL
                     LDKTHLEARLGMGTLAFDKETLFELDKAMAVPSGIIVMSGPTGSGKSTTLNAMLRELN
                     HPEDNILTLENPVEDEIPGVTHCDLRNSSEFKPMISSFMRSDPDIILMGEVRDYESAE
                     LAIEAAITGHKVLTTIHTPRASQIIERFQQLGIERWKIAQTLKAACAQRLVKLLCPAC
                     RERRDGVPEREIRLFHLDPSWATRPVFVHRKEGCPECKGRGYSGRTAILEILPISPKI
                     GDKLAKGEITPFELELEVRKESSLPSLRDNGLKIMAEGKTDLDALKKVLDMTYDS"
     gene            complement(41740..42327)
                     /locus_tag="PLP58_00195"
     CDS             complement(41740..42327)
                     /locus_tag="PLP58_00195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ06969.1"
                     /translation="MVAEAVNGIPLSATVPGLSDIRPVLERIAVTCVKHLRLGGYLSE
                     AEAALLTSSYRESICRPSPPQDALIFIAETQAEKHSLTVEGEVLRMQKILRASLSDRV
                     HYWSFGPLPGRADALYEHCPALRPICGALGCPAIMAGETSIVHVASINPVAATVASFW
                     IGHELNRVTAGDSPFVFSFLTDLASWSQLMQRHFA"
     gene            complement(42334..44157)
                     /locus_tag="PLP58_00200"
     CDS             complement(42334..44157)
                     /locus_tag="PLP58_00200"
                     /inference="COORDINATES: protein motif:HMM:NF012485.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ06970.1"
                     /translation="MSALFTRWISSSLASLHLMGAAQDPAATGTLDAASPAAVDVSSR
                     LGAAASTVSSLPALPDDGYWIDNAPINEVFQYLARSAGLQFFHNNELATPAFNVTGHL
                     KLDNPRQQMEDLAQAYGLTVYQQRSTVSLLNDAQLSRLPLEVMSYELKYLRGAPLNRA
                     NQGGGGGGGGGGATVDGMDGGGGTGGGGGGSQQADFEKLKAIIRPLLTREVGQIEFEE
                     KTNTLLVTDNQVRLERIRALLAQLDKAKQQILVNVRVLRIKRNDARRAGIDWSRALGD
                     TGTTITASQSLNALFNLPDISTITRSGALPNPTTSINKVNTDGIGLVFGNPQVQAILR
                     ALEEADIVSQEACPTIITEDNEQGTISIVDRFPVITANVTNSGAGVQNISELVRYQID
                     EKDPDPTTNPEKSREIGVTLSVTPTLLPDGTVRMKLRPRVAKIVELVAGQGGNVYPRV
                     SESTVEAISRIPAGQSLFLGGFYDYSGSEGENKVPVLGSIPVIRKLFSTEQRKLEQVS
                     LVFIITPQIYDASNAAELPGVNWQMREYSAMQPEDIADSGLSLTGDGGGNQGLAARAA
                     PPASAPRESWLRRVFTKKSSPAMVQSPAAGPSGTHGGAPYR"
     gene            complement(44154..44753)
                     /locus_tag="PLP58_00205"
     CDS             complement(44154..44753)
                     /locus_tag="PLP58_00205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ06971.1"
                     /translation="MRTALTIMTLFSFALGAGAQKPADARPKRSALFIGKGTASWLPS
                     VEILPTALDIEAAAKNLGARQRNLDPFGLAVFPREDAAPVFFDDTLREAPRVTLNQAL
                     QSLKINGVNLARKEFLIGGRNVFEGDVIEMTFKGETFQAQVIEVGHTELRFRNLLRDE
                     TGVLLHSMIPALPLEPMHKIASRLDGKVSPMEPANPKSP"
     gene            complement(44750..45310)
                     /locus_tag="PLP58_00210"
     CDS             complement(44750..45310)
                     /locus_tag="PLP58_00210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ06972.1"
                     /translation="MSAYSQPIIRFGLVTPAVFNVLLLGGVLAALGKLADIRKEKEER
                     FEEQTQRLAAMKKLEADIAPKRKTFADQKKLLETDPGQLFTRVLDAVLPKFKEIELAR
                     GSLVFPLDRGRVARSLRTDAARVKSSFSGGFGPMQEALLQVESLMPQAVLEDMKITRK
                     AHLLTQQEHLVMEMTHTCWKAGEAKP"
     gene            complement(45319..47205)
                     /locus_tag="PLP58_00215"
     CDS             complement(45319..47205)
                     /locus_tag="PLP58_00215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ06973.1"
                     /translation="MLSITDPCELKAADIYRQARAVASKTSRGTDGDWYREPDDLAWQ
                     WHIWFDGGREVASAAFSRLDNQWRPGPALPLASLTTAENASELLGELISTSYYSQKPK
                     SLGVILHVADEFALAEMAQTLEAAAEGGEDFKILRYNLVDEPLEMLADREVSTDTTSW
                     RLLPFWGAGPGQTRSVAMALSRAREPFMQKLMAEGEKMRLPVRVSVTSAPLEALAALP
                     LIQPEARGGRLVVMPYLKYTAVFALSPEGELRAARSLSHRGGSLTPAGFGDILWNMAV
                     AAELAGADHAGKPRLLLASSNQRAVEAAAKDLELYSLSRQELECEILDLGSLPQLAAI
                     PGNLMELMIYDSAAIEQVRAGGAPLGGTETFPALWQGWAGQNFMDLARLDTLYPTLSD
                     LRMLRFSTWFVVLLVFTLVSTGAYGTWSLFSAMNHPSWELTESQVSMTKAKHAKLIDE
                     QTQIQLTEQLLLPRSRGWTTLEFLLQLFPEDSGVRLENFSYSLEAARSAASSSSRSSK
                     GSKGKGSKNDATGLVRNWTIKGLVKPKALELLNSLNSQRGLGAFFEQVAGATDDPGFK
                     PDPARQITVSLTQGRNSRYDSQATAADAARDPTLAFPFNFEIQISQTISEKDTLALPL
                     EKPF"
     gene            complement(47255..47689)
                     /locus_tag="PLP58_00220"
     CDS             complement(47255..47689)
                     /locus_tag="PLP58_00220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HRJ06974.1"
                     /translation="MKTHINRTNNTRLAKRLNASGMTLIEISLVIALLLGLIAVVFLG
                     LGTYRAGADKAKCKMQLAAVQKAVRAGANFQNLDIGADLESTDVFGAGLLMENEPACP
                     SGGEYAWEATVPAVGTPYGNCDYEGTGATSTHVLVEADTTDW"
     gene            complement(48006..48863)
                     /locus_tag="PLP58_00225"
     CDS             complement(48006..48863)
                     /locus_tag="PLP58_00225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009959485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin family protein"
                     /protein_id="HRJ06975.1"
                     /translation="MITHTARPLKVVLYEGNGAIPLPQEARAKVMMALLDKGYSVSRV
                     TPGASAAPRPADDHTLLVLGAFEHRQAPAIEDAAGRIKIEARDITGLEAEGVVSLVEQ
                     TRGARPMNEPGKWKPWFPVIDYTRCTNCMQCLSFCLFDVYGVSADHKIQVQNNDNCKT
                     NCPACSRVCPEVAIMFPKYQGGPINGDEVSAADHGREKMKVDISSLLGGDIYAALKDR
                     SAAAKSRFSKERSPDKALDERKKCLTKLVSDGFIPMDVLASLPSPDEILRKAEEAKAK
                     AAAALAAQA"
     gene            complement(48923..49879)
                     /locus_tag="PLP58_00230"
     CDS             complement(48923..49879)
                     /locus_tag="PLP58_00230"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014673.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HRJ06976.1"
                     /translation="MARALHDPATGYYARRIHTVGPRGDFSTAATLSPALGQAVAAWI
                     QMQTRFMPDVRHVIEVGAGDGSLMASVRKVSGWWRRRSLRWHVVENSPVLARAQRRRL
                     GSGVKWHSRLEDALSAARGRAFIYHNELLDAFPVHLIEWQDGEWSEIHLDQGAETPCP
                     LDWPEQERSQFSALREWQNPPPGQRCELHRPVRDWFRSWAPAWQAGAMLAIDYGDLFP
                     ALYHRRPRGTLRAYLLHQRLEGAEVYANPGRQDITADINFTDLRSWTTSLGWQEHEYM
                     TLADFFRAHLKPASLAALPPSFLHPDGAGGAFKCLSAAKVAA"
     gene            complement(49924..50568)
                     /locus_tag="PLP58_00235"
     CDS             complement(49924..50568)
                     /locus_tag="PLP58_00235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ06977.1"
                     /translation="MSTPSPAKTKTLLIAGAAGLVIALWFGWQWAGKEPEAPAPVTKT
                     KDKAGTKTEPNLLSTPQPTTPPAEKKTEPGQQLMTAEEGDVLIDEILRSDKEIPQMAR
                     DLHELVKKLNGEAQVNASHHLVNLTSDEDYGLIAGLLVDQKTNPEVLDVLFSDLMNRN
                     RELQMPLFMNILKNPQHPQSQEVRDVLTILAGEDFGNDWAAWDKWAIEELQANQ"
     gene            complement(50624..52216)
                     /locus_tag="PLP58_00240"
     CDS             complement(50624..52216)
                     /locus_tag="PLP58_00240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009962375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - ribonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rne/Rng family ribonuclease"
                     /protein_id="HRJ06978.1"
                     /translation="MPLGIVQRIKELITGKKDIRQGTRLVINCEKLENRVALLDNGIL
                     EEYTIERTGTSTIVGSIYKGRVKNIEQGLKAMFIDIGLDKNAFLHFWDAIPEALSNQG
                     MEEVSRGGGGKKRKRIEAKDIPSLYPVGSEIMVQVTKGPIGNKGPRVTTNISLAGRLL
                     VLMPQNDQFGISRKVEDPKERARLRKIVEKVNVPEGMGLIMRTEASGKRDRHIIRDLS
                     LLIEEWNEIVAVRDGHKAPVCCFQEPELIERTVRDFLTEEIDEVACDDLPTVERMQAM
                     AAHISRRAKNRITHYQGQTAIFDHYGIQKQIDNAFYRQVWLPCGAYIVIDQTEALVSI
                     DVNTGRNKGGKDVDKLILETNLEAAQEVARQLRLRNMGGLIVVDFIDMKHRKDQQAVY
                     KAMLEHLKRDKAKTQVLPISQFGLMEMTRQRLHESLSSALYEVCPYCKGHGQVKTATT
                     MSVELQRRLSAILGTGREENRNLIVVVHPDVMQRLKTEDGELLVDLERKYLARLTFRS
                     DPTLHREEVKISSSSTGEEIRN"
     gene            complement(52247..54532)
                     /gene="mrdA"
                     /locus_tag="PLP58_00245"
     CDS             complement(52247..54532)
                     /gene="mrdA"
                     /locus_tag="PLP58_00245"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR03423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HRJ06979.1"
                     /translation="MVVKYRLRLHLLTLAMLCGFGLLVYRLWSLQIDRHEEFVNKVPE
                     ARQQRARIPGVRGEIKDRNGIVLATNKAGFEVQVNLRDVYDEYVRQCKIKKMDIPTVD
                     FEFLDRGLPRKRKEADIVTMFQELIVSRLNEMGLAAEFNAEQDLRVHYRSFGGAIPWV
                     YRDNLTFAELSHFAEHNLALPGVTVAARGSRVYPLGSLACHLMGYVRMPDDQRVSPEE
                     RKGWDYYVPDEYGGAGVEKSFDSHLRGKPGVRVMQLNERGRPVGEVSYEEPRKGDDVY
                     LTLDARIQYIAERALIDGKIGRGSVVVVSPQNGEVLAMASVPNYDPNRFIPSIKQADW
                     NELLGNHTVPLLNRSIRSFVPGSTYKVPIALAGCLAKIQGHHFNCSGSVTYGTKAMQC
                     WIQRQSGGSHGSLGLSDALMRSCNCFFYQYGNTAGIDNITRVGKMLGLGERTGIELEE
                     DDGGILPNPDWLRQASPRERWSNGHTANVSIGQGSVLASPLQMACVTAAVASGKSWKP
                     HLLKKVMEGDHPVFEKQPEMRADLAQHFPAKDLETVRNGMWKVVNAQGGTARAARIEG
                     VEVAGKTGTAQNWRRDERNVRVEDNHTLFISFAPYVNAKYAVCILVQGGKSGGGCAAP
                     VAQRVLEQSLALDNGYEIKPEPVEEVKGNFNKIDMVSYGDTPNLLAALAAQEDGDTGT
                     ESERESTDTSPRGRQTPAPSIKREADTAGRAAVKNAAEVPKAVPVRRAGIFGRRSADQ
                     PSQPSEQPAQGGGLLRRFFRQ"
     gene            complement(54522..55133)
                     /locus_tag="PLP58_00250"
     CDS             complement(54522..55133)
                     /locus_tag="PLP58_00250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ06980.1"
                     /translation="MVFYPVTFLLLLLTFVVQEFIPGIPMAQFATLFLPPVFFFAASV
                     AVPFPMMLVLAFVTGGLWDARHMPVAASVASTTSGAMMTGASSEAAAPGAFGFGISIL
                     LFGLLGSFLQGVRPLFKRGRLELPVLLVGFTTFAWLLTEYLVMTFLRGSLFFPPGVWV
                     KMVTASLLAMLASPLIFLLLYSLAGLSRYEIKYEGLTFSFDGR"
     gene            complement(55141..55956)
                     /gene="mreC"
                     /locus_tag="PLP58_00255"
     CDS             complement(55141..55956)
                     /gene="mreC"
                     /locus_tag="PLP58_00255"
                     /inference="COORDINATES: protein motif:HMM:TIGR00219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HRJ06981.1"
                     /translation="MNKLNILALGIFLAGVVSVFTLDTPVTRDIQGRVMAALSPFIHS
                     SAAIESGVAEAAAPALDPRQLKIENERLQIEVERLRIVSQQFAQTQEENNKLRELIEF
                     KQASPFKLAGARVVKRVSSTWWNSMIIDKGSLDGLATDSPVISASGLVGKTGKLAPRM
                     AEVILLTDEMCRVSAKVEGTLEQGILAGERGGLDMRPDLRLQFLGKGAQINPGANVIS
                     TGEGGVFPPGLLIGRVKRFEIKDLYGVAVVEPAADFATLEHVFVVEMQSDTEP"
     gene            complement(56024..57055)
                     /locus_tag="PLP58_00260"
     CDS             complement(56024..57055)
                     /locus_tag="PLP58_00260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018969829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000902 - cell morphogenesis [Evidence
                     IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HRJ06982.1"
                     /translation="MLGKLFGFVAQDIGIDLGTANTLVYIKDHGIVLREPSVVAVKTG
                     TNEVVAVGDDAKRMLGRTPGGITAIRPLKDGVIADFRVTEAMLRHFIRKVHNNRRAMR
                     GPRVVIAVPSGITEVEKRAVKESAEQAGAREVYLIEEPMAAAIGVGLPVQEAAGNMIV
                     DIGGGTTEVAIISLSGIVYSRSVRVAGDELDEAIINYMKRAYNLMIGERTAEEIKLRI
                     GSAFPLGKETTMEVKGRDMVAGLPKTITITSQEVREAMLEPLNAIIDAVRTTLERCPP
                     ELSADLVDRGIMLAGGGALLRGLDKLLQEETALPVHVAEDPLSAVGEGTGRVLSELEL
                     MRKVSSTEV"
     gene            complement(57294..58532)
                     /locus_tag="PLP58_00265"
     CDS             complement(57294..58532)
                     /locus_tag="PLP58_00265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ06983.1"
                     /translation="MSATRISIHLISVALLLLWSAVLLYFYATGRIAQYLPGDGIFRP
                     MVLVSGIGLAVLALFNLLTAGAEDAGCEGHDHGGHDHNHGHDDCCHGHSHAKTSPCGH
                     NHPHHAHEGPCDHEHPHHAHEGPCDHEHHHEHEDHAPGCHGHDHAEKPASCGHEHHAH
                     EGCGHEHHHAGGHSHGHGLLEESSWPGRVTAILILAAPITWAAFQTPDNYSANAVINK
                     GLYNPNYNSTANADKFSLRTDTTSAAAKPATAEAPTPAATASAPQTAAAADGKQPQSY
                     GSFTLEDLKQQVPQSKEGNFMLDVPEIYYTGGDIEVQTVLTGQPVETIAQVLPEKVKN
                     DDGRRLRIFRMLVQCCAADARPYSVPVEFEQKAPDFKDMTWVKVTGIVSYEKDGSQTV
                     PVLKATSIEETTAPENTMIY"
     gene            complement(58529..59509)
                     /locus_tag="PLP58_00270"
     CDS             complement(58529..59509)
                     /locus_tag="PLP58_00270"
                     /inference="COORDINATES: protein motif:HMM:NF015715.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HRJ06984.1"
                     /translation="MIFATLPEPSRLGDVLMAFLSILFEGAPYIMIGTLLSGVIDAFL
                     PSKLLDRLLPRNKVVSTFMAGFLGLIFPVCECAVVPVIRRLVQKGLPLSCALTYMLSA
                     PIMNPIVAVSTLTAFKEFQAVTGWMELGNATMTIARLSLGYTVAVIVGLIVLRFRPGQ
                     VLNANIVGGIESARAGGHAPKTTFNGKLVHAMRTAMRDFLDTGMYFTIGVIITSVFNT
                     QVNQAILDSVAGNEWLAIPSIMALAYVLSLCSTSDAFIAAPMAVFSQAAKLAFLVFGP
                     MMDIKLMFMYAVVFRRRVVVLMLLGTFLLIAFLSNPWISLVELLQTSSKP"
     gene            59685..61016
                     /locus_tag="PLP58_00275"
     CDS             59685..61016
                     /locus_tag="PLP58_00275"
                     /inference="COORDINATES: protein motif:HMM:NF024753.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HRJ06985.1"
                     /translation="MKATRLLVLLALSLTAARAENWPNWRGPAQNGTSPETGLPEKFS
                     RTEGVKWAADVPGISASVPVVWADKVFLTAPIAEEKKLVGLCYDAKTGKELWRRAISE
                     GGLQWDNKSNLASPSPVTDGERVIFLFADAVAASYDLSGNLQWRRDFTQSHGAFATQW
                     TYGSSPLLDSGRLYIQVLQRDEVFDFQGFQKGTPGKDMSSYVLALDPATGKDLWKVVR
                     PSEAVAESLEGFSSPVCHDVNGQRQLLIVGGDCITGHDAATGAELWRWGTWNTEKIGH
                     WRLVPSVVAGDGIALACAPKKNPVYAVKLGGKGTAELAWTSDPKEASSDVSTPAFHDG
                     KFYILDSDRRSLSCLEAKTGRLIWQGDLGSKAKFEGSPTVADGKVYMTNFWGQVYVAK
                     AGGDKFELLAVNEMGDGSKPNGDASSVRSSIAAANGALYIRTQDKLFCVGR"
     gene            61067..61699
                     /locus_tag="PLP58_00280"
     CDS             61067..61699
                     /locus_tag="PLP58_00280"
                     /inference="COORDINATES: protein motif:HMM:TIGR01509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HRJ06986.1"
                     /translation="MQSSTAPQPVLLSDIGNVLAFFDFGIAARRVAGRSPFPEVALSA
                     RLDHIKGPFENGDMDDATFLREAVAALQFQGSEAEFAEIWCDIFTENTAMRRTLDPLA
                     GRLPMRLLSNTSGLHKEHLLSAFGIFLPFGGGVYSYSARCSKPGEEIFRKTIAQLGLD
                     PALTFFVDDLEPNIATAARLGFHTHHYQPARHAAFERDFHAWLARAGISA"
     gene            61798..65310
                     /locus_tag="PLP58_00285"
     CDS             61798..65310
                     /locus_tag="PLP58_00285"
                     /inference="COORDINATES: protein motif:HMM:TIGR02604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1080 domain-containing protein"
                     /protein_id="HRJ06987.1"
                     /translation="MRLTTLLLVPLLLSRAWSATPVTEEINILDLMKKGGVEYHINPD
                     QDFADPPNEIFQLKGEELHISGRGYGYMVTKESYKDYHLVVDFKWGPKTWGKRADRSR
                     DNGILVHSYGPHGAYGNTWMASIEAQIIEGGIGDILVLSPKLADGTELITSVTAEIEL
                     DRDKEKRWKKGAPRQVVTKGRINWEKRHEDWADKLNIRHPDDPDSPVGEWNRMEVIAK
                     GDTLQYFVNGRLVNEAFDCKPAEGRICIQTEGAEMIVRRYELHPLGGFTEKWSAIQAS
                     GGSDISVRHGQEKALTPEESLAQIQLDGPYEAQLVAAEPLVLDPVEVTWDDKGRLFVA
                     EMRDYPLGPPKAGDPWMSRISQLVDENGDGRMDKSIVFADQLDNVQGLLPYDGGLIAT
                     TRTQILFLKDTDGDGKADVNRPLIKGFNPRHSQLQVSAPRWGPDGCVHFNNGLDAKEI
                     YPADAPDKVVGVQGANFKWNPKTGEITPTGGKGQYGGAFDDYGHHFYCSNRNPLMFTV
                     MPYEVMRRNPHAGITQVHEDIAPPGAETRVFPLKITHTTADAHAGTNTACSGLGVYRG
                     DLMPELRNNVFVPDPTGQLVTRYKIEPNGASLKATRVGERTEFFRSGDEWSRPVNFTT
                     GPDGAIYVCDIYRRWIDHARFFPEDFVKANDMRQGEHQGRIWRIVPAGVQASAAQKSS
                     PERLKAGHPHHPGRYHAIPEAGWGEQPAEHKERALLFRILQKHDEGQVSKPEEISFRA
                     KCIADHPEDEWLSKAVLSSSEYTEGRVLKAVLQSPNFTSRFSPARSATIRAFAAGAIV
                     SGDPSDVAECLRCLELDKAELLWWKAPLLQGVIDGFAKGYNVEPKSLSAFMALPTPDL
                     EASSRGMLDLELAEFQSIAKEIASLQSQIDTIIADSKAPLDQRLAVLPLLASRKWDAV
                     QTVVKSLLTTAQPQELTTATVALLKKFSATSTAPLIYELLPTAGPALKRDLVPILTGN
                     ATTALALFKRMDQGEFPTAWVDVETRWRYQRGKGEMADLAKKLFGEASSDRAAVVKKY
                     MAATTLKGDSEKGRAVFASVCITCHKHGDLGVDVGPPLSDVKVKPPEALLSDILDPNR
                     MFEARWSAYLVETKDGRTLSGLIQNENTDAIVLAMMGGATETLPRSAIKTLKSLDRTL
                     MPDGLEAAITPEQMSDLLAFLGGK"
     gene            65527..66906
                     /locus_tag="PLP58_00290"
     CDS             65527..66906
                     /locus_tag="PLP58_00290"
                     /inference="COORDINATES: protein motif:HMM:NF013567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HRJ06988.1"
                     /translation="MNRRAFLQKSALAGTATALSAKSYAQVAGANERVRVGMIGFGLI
                     GRIHTRSFHGLKDSCQVVAVSDCFAPRMDACVELVGGGCAKMPDFRRMLERKDVDAVV
                     ISTGDHWHGLQTMMACDAGKDVFVEKPLHLFVREGEWMQKVAARTKRVVQVGTQQRSA
                     AHYHKAKELLRGGAIGDVVSVQCNFFRNVMPGIGSPPDGDPPAGLDWDMLLGPAPQRR
                     YNPNRALYHFRWFWDYSGGQMTNLGHHSLDIVHWIFDIEAPKAVSSSGGRYFLKDNGE
                     VPDTQDAIIEYPKFNAIVQFRECAAGAGATAMGALTFMGTKGTMTLGRDGFEIIPDKK
                     IEPVNAFAHIIGGHPVGGPQPAPEPEGDFWCEKAEDKSGDWKQQYIEHAKNFLDCVKS
                     RKTPHSDLASSHWVSTTCHLANISACTGRKVVWDAAANDIRDDKEASAMLTRPYRAPW
                     DAELKALIG"
     gene            66903..67922
                     /locus_tag="PLP58_00295"
     CDS             66903..67922
                     /locus_tag="PLP58_00295"
                     /inference="COORDINATES: protein motif:HMM:NF013429.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HRJ06989.1"
                     /translation="MNRRHFITAASLAVARSFLSAAGAGGSRLGICTFSCHQRWKLSK
                     PVDAVEFYRYARELGAEGVQAPLRGCEPKTIRDLVEQSGGYYEGELRLPKSAADLAAF
                     EAEVRQTREAGATVARAVFTSGRRYEAFSTLEAFQGFHEQCKRTLALIEPVLKKHRLR
                     LAIENHKDHTTEELAGLMKAISSEWIGVLVDTGNNLALLEEPHEVVEALAPYALSVHL
                     KDMAAQASDDGFLLSEVPLGSGFLDLPRMVSVLRKANPGIVINLEMATRDPLRIPCRT
                     DAYFTTLPGRRATHLDAALRRVKENPPKGPVPAVSGKSSDKVLADEERNNRHGLQWMK
                     QSLHS"
     gene            68005..68199
                     /locus_tag="PLP58_00300"
     CDS             68005..68199
                     /locus_tag="PLP58_00300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ06990.1"
                     /translation="MLDPNHAANETLFRERLGNEVVRDGRWKLVRCCNDIRDYNPQTK
                     RGSGSGTHTGGSLCAPCLWG"
     gene            complement(68250..69053)
                     /locus_tag="PLP58_00305"
     CDS             complement(68250..69053)
                     /locus_tag="PLP58_00305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ06991.1"
                     /translation="MPLTTLQKQVLRVLAANRSEESHFAGGLVLNSADDSPRFSHDFD
                     IFHEAESEVARASDLDVAALRAAGYAVEHAVGDWAAHTSFRKARLTHEGEALEIDWAA
                     DSAFRFFPIQRDDVLGWRLHLFDMAANKALALAARSVTRDYVDIVELDRTYPLEAVIW
                     AACGKDEGFSPMLLLDMMRRFARINPRQLETIQARQLDPIVLKKAWIAMSDRAEAEIT
                     RLADTRPEVPIGVAFVNARGEPGWIGSDPSLRIHRPSVRGCLPVVQGVE"
     gene            complement(69044..69262)
                     /locus_tag="PLP58_00310"
     CDS             complement(69044..69262)
                     /locus_tag="PLP58_00310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ06992.1"
                     /translation="MNTEPPATTSQPVSAEVAEMARQAVRDFHECFWWWNPDFTPQTV
                     EEVREVVLNLRKGGHRAWQRAQELNSCL"
     gene            69403..70518
                     /locus_tag="PLP58_00315"
     CDS             69403..70518
                     /locus_tag="PLP58_00315"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014110.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialidase family protein"
                     /protein_id="HRJ06993.1"
                     /translation="MKKLLPLLLAVSAFAANESPEDARLREAIPFGGDFKPNPKVGAF
                     VVVGHGARILVSKDDGKTWTQAFFAAPGADHGPWATKAVAYTGGVFVVPVGWGAPTIY
                     LASDDAVNWRHLTKGTAKLPEGRGDARVMPGTWGIAAGKGTFVGSGYMTFCATSDLGQ
                     SFSSFGMWGAFKDDPRGKLSTHHVGAVYCGDASGRFLALGDNRGDSAAKFGNLFASDD
                     LGKTWKWLAPKGLDASTGRGEMLSNGRLLVMTDKDAANAWTSTDAGETWTGPHATGTQ
                     RATLSVVGSEFWLCGKKSRASTDGQTWRDLPAAVPEGKIIASDQGTLISIHQQRTNIQ
                     RSTDGGKTWQQVHQYTPPDFKGGAQGLRAGAWGLVAP"
     gene            70605..71597
                     /locus_tag="PLP58_00320"
     CDS             70605..71597
                     /locus_tag="PLP58_00320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ06994.1"
                     /translation="MKSLLFVLPLLVATQRVKAIDPFFPPDDWNAYQQRIIKDREHEK
                     PGWLRPAPGTSPAVAIQTLILPEFKVENVSLEVAFQAWQDACRQGGINVRVYLGPAAL
                     INRPSITHSAKGETAARTLAFIENLGLARVRLLEDHFLAGANSLDPHELRFRVWVLSE
                     MAAGFLDLHKLKPAQTGWCSADSALTQIGAAFPRGAYADYHEQMRALVAINTTVTLDA
                     LSEWIEEMEARAIIQQFKDKEEVQMDGYRLAVRSQQLDARNAAKIAARLRDAKGRLVP
                     FAASSQTWAKSRGLITPAGSAVWLDVASSTLWLRSRPESLDQFMPQLQKANLER"
     gene            71619..71894
                     /locus_tag="PLP58_00325"
     CDS             71619..71894
                     /locus_tag="PLP58_00325"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HRJ06995.1"
                     /translation="MKTLLAALLSSFLILVSSFAAQPRPNILFILTDDQGWATLGCYG
                     SKKVPAPNLDRLAGEGVRFTDAYLPPQCAPTRAGARKFATGSISLSC"
     gene            72106..74442
                     /locus_tag="PLP58_00330"
     CDS             72106..74442
                     /locus_tag="PLP58_00330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009965736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HRJ06996.1"
                     /translation="MPLFLPLSCHRHPAPVPWLSGEKRTVQDGRAVFPLEMGGVRRLV
                     PAHPAAAMPNEKPVSLKHLTLLGKENIPQAGGYLILPARLDYLDVLRLEAVLEGRNIV
                     YLCEQGAALHPMLRAHLEREGAQVLVISPDLTDTAALRKAVTGKTAQDAVIIFLPAQA
                     ACLNAPLCTIPGMRLEFLLKAGCPVLPLHVLHPREVALDIGPRPDETAVVMSFGMLLT
                     GEDVSLASYQESLLELCRQAFETHPALPLHLACALIQGFKRHGRNNVVVDGKDDKEMG
                     FDTIFAAAIALSRVIKSETAKERVGIVLPPGLGGLIANVAVLLAGKIPVNLNFTAGRA
                     AIEFAMKASGLDRLVTADPVVRKMQTFPWPPTRQLILVERLLPRMKTKIGLWLALSKL
                     LPASLLAALLGVPRHGGDKEALLLFTSGSSGNPKGVVLTHRNIMANVIQFGSRLGLKS
                     DDAILGSLPLFHSFGCTVTLWYPVIAGVDLVTYPSPLETKRLAELVEKHSVSVMLATP
                     TFLRGWLRGVPRESLTSLKMVVTGAEKLPATVAEAFEARFGKRVYEGYGLTETSPVSN
                     VNLPDPVPLAEEGHAWLPAHRPGSVGQLLPGLAVRITEPEGGRRLSVHQSGMIWFKGA
                     NVFHSYHNDPKRTADVKDEDGWFRTGDIGRLDLDGFLYIEGRLSRFSKIGGEMVPHET
                     VEEAIIKAMGLENESARKIAVVGVPDPDKGEALILISAMTGGPENQEILDLRYRLLEK
                     GLPPLWIPKKMIRVSDIPILSSGKLDVQSCEKLARGEV"
     gene            74473..75222
                     /locus_tag="PLP58_00335"
     CDS             74473..75222
                     /locus_tag="PLP58_00335"
                     /inference="COORDINATES: protein motif:HMM:TIGR03008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CAAX prenyl protease-related protein"
                     /protein_id="HRJ06997.1"
                     /translation="MKLMLETWRSSPASAHVAPFAVFMTFLAVPGWFRVENPELPWHV
                     RAPELWVYPLQTLVCAGMLFFFRRHYALRPLRGLGLACVLGVAGIALWILPAWAYQKL
                     GTENPPMWWAWIGMADRSQGFDPGQLAGWPVWERITIFMRFARLVIVVPLVEEIFWRG
                     FLMRWLAAEGRPWHETPFGAHTWRSFWIVTTLVMLAHQPADWPAAFVWGALVYLAAAR
                     TRSLGACVLMHALANALLGAWVMGTKQWGFW"
     gene            75311..76360
                     /gene="argC"
                     /locus_tag="PLP58_00340"
     CDS             75311..76360
                     /gene="argC"
                     /locus_tag="PLP58_00340"
                     /EC_number="1.2.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007417930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003942 -
                     N-acetyl-gamma-glutamyl-phosphate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-gamma-glutamyl-phosphate reductase"
                     /protein_id="HRJ06998.1"
                     /translation="MSKPVKVAVLGASGYSGIELLRILLRHPHAELVAVTSRTLAGKA
                     LSAEFPRFRKVGVADTLVFTEPDVDALKASGAQAAFLALPHGVSAEYAAPLLEAGMRV
                     IDLSADFRLRSAEVYKEFYAHEHPAPGLLAKAVYALPEWRAEEIKNARLIACPGCYPT
                     SILLPLLPLLKAGLLSEDPLAVSSMSGASGAGRNASVPLLFCEVQNSLRAYSVPSHRH
                     LSEITQELSLAAGREVRLTFVPHLVPVFAGICTTTFAAPASGVSLDQVESALRAAYDG
                     QPFIRLLGRNQCPDTKHVMGTNFVDIGWALDERAGRLVLMSAEDNIGKGASGQAVQNL
                     NLACGFAPDAGLLNA"
     gene            76357..77655
                     /gene="argJ"
                     /locus_tag="PLP58_00345"
     CDS             76357..77655
                     /gene="argJ"
                     /locus_tag="PLP58_00345"
                     /EC_number="2.3.1.1"
                     /EC_number="2.3.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009960523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004358 - glutamate N-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutamate
                     N-acetyltransferase/amino-acid acetyltransferase ArgJ"
                     /protein_id="HRJ06999.1"
                     /translation="MSTTTMDCDPFREIRVPFKVIKGGVTAPRGFRAAAVSCGIKNPE
                     AARLDLALIVSDTPAVTDAVYTTNKVRAACVRVSQIHIRESDTRAIIANSGNANACTG
                     PQGIQDAKAMCKAAAEALGLRMRQVQVCSTGIIGMNMPIQRVTPRIPELAAKLSPTGS
                     DDAAAAIMTSDTKPKSFAIEVPCPGGGSFRIGGIAKGAGMICPDMATMLCFITTDAKI
                     SKDEIKRAMRCAVDQSFNCITIDGDTSTNDTVIVMSNGQASAPAMRKGSDEAKIFRCA
                     LHKVMLELAKMIVTDGERVTKFVEIRVRNARTHADAKKVAEAVAKSMLVKCSFHGSDP
                     NWGRIIHAVGYSGARIREEMVDIYFGGLLACKGGLTSGTPVAELEKVVKEPRFSVTID
                     LNLGSANHIVYTSDLSEAYVDFNSSEYSAAVHARRQKGLA"
     gene            77819..79141
                     /locus_tag="PLP58_00350"
     CDS             77819..79141
                     /locus_tag="PLP58_00350"
                     /inference="COORDINATES: protein motif:HMM:NF019222.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1552 domain-containing protein"
                     /protein_id="HRJ07000.1"
                     /translation="MISRRKLLRQIAATAGAALGSPLLPNISQAASRAHGGGPKRVIF
                     FLQNQGFDPATCVPAGMKNSGSLARAKLPEPIEALEPFKERLHIINGLHGLHTSPSHS
                     AFFGALGGYRGSDGVPPSASTIDYELSKVLPETLLPHLCIGMDSMENMRAKPTVANLS
                     ASGAGQPIFMHSNPNHLYQMLYGGISDGDIRKQHEARSSMFDRIEQLAAAHGKALPMA
                     DQQRYGQYVQGFQDINGLRSRLNGVSDHLRNFAPKVDDRYTKPEFETDWHDVLLDLGI
                     SAIKSGITSVLTIGSGRGEIFGSWKGLGVEEQGHNLGHMKQPDNPIWIKIRQYNSRML
                     VKLMEELESVPEGSGTMMDNTLIVYTSNNADAQHTSGATWPVMLLGNCGGIFKTGCFT
                     QLDGTRPINALYATILKTAGVECERFNMSEKMAAKFDSGTGPLREVLV"
     gene            79535..79936
                     /locus_tag="PLP58_00355"
     CDS             79535..79936
                     /locus_tag="PLP58_00355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ07001.1"
                     /translation="MKRFWQILALLMLALMVPASMCCYGADGCKADVCCSEESHEHED
                     HEGGEHHAPVSCPSDTISHSQVPAPVTMPEMQMVELAELIQAMIRLQDDLATATESSG
                     LPMTTAPPELRATWHFTQRTALPVRAPSIQA"
     gene            80062..81333
                     /locus_tag="PLP58_00360"
     CDS             80062..81333
                     /locus_tag="PLP58_00360"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HRJ07002.1"
                     /translation="MRLFILSSLLLAAVPQLLAADSKPEPSSISSLVGRTLAGNPEIR
                     FYEAEVAAAKATRSTAGRQSNPELSLQVGNNHIRNADSRSNGLAFSAELAQPIEWPGR
                     LGLRKAIANRDIAMAELGLERFRFHLAARVRVLAFTLSAQQDLANAANEVAARYASLR
                     EVMVQREPAGIAPQLEIATIEAAALVAESRAAKAGVVVQKALLELNQLMGRRADAPLI
                     VKRGPLDAKAAPALLSLLGSAMKNHYELRLRRAELEQQGFKVELAKNERYPAFTIGPF
                     VEVQNTRTLDDQTVAGIGISFPLPFWKNGKAEVSNAEARQMQAQATLAAAQREVERQV
                     TESMLIYQTQQARLAVWKGDAVTKFSDAAALADRHFRLGAVPMSTFVELQDKYLEAVE
                     AIHSTQTEALEAALTLEELTGAPGSLITTKN"
     gene            81346..82044
                     /locus_tag="PLP58_00365"
     CDS             81346..82044
                     /locus_tag="PLP58_00365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ07003.1"
                     /translation="MKLLLILSLIVTAAALTSCSESSASPSTEHTAAPENGAQFKEGK
                     GVALTALMAESIRLQTAEVQEEKIAPAFTLSLQAMQRGTEASGWLTADQAARVQPGME
                     VELGTAKGHVLRIEKAAYATLGDFEVTVQTAAPIEAGSVITATFRFPAGDAVTAIPQA
                     ALLTTAEGTFVYAKNDEFFLRTPVKVGARNGDHVEITDGLYTGDEIVTTPVMSLWLAE
                     LQVLRGGKACTCGH"
     gene            82054..82503
                     /locus_tag="PLP58_00370"
     CDS             82054..82503
                     /locus_tag="PLP58_00370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ07004.1"
                     /translation="MFSIAIHHRQQRRMITWIMLVVLLPLFAVPWSQAHQGQCSPRMP
                     ACEMTCCKDMSSDNTTHHSECPCQDTDQGCDCACCLHIASTMPPVVMSAPQTCPLNAH
                     SIRPWSQRAPSRTERPPLPPPKQAHHFHPVGTTPTSVHSNHSNLTYL"
     gene            82500..82733
                     /locus_tag="PLP58_00375"
     CDS             82500..82733
                     /locus_tag="PLP58_00375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ07005.1"
                     /translation="MKTLFLSLALILTLTGLYAATQSSTDAKSNYCLAAACCTGGACC
                     ATGDCCCLTGSCCEAGLCSCDPGCCTGCECCAD"
     gene            82792..85938
                     /locus_tag="PLP58_00380"
     CDS             82792..85938
                     /locus_tag="PLP58_00380"
                     /inference="COORDINATES: protein motif:HMM:TIGR00914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0051139 - metal cation:proton antiporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CusA/CzcA family heavy metal efflux RND
                     transporter"
                     /protein_id="HRJ07006.1"
                     /translation="MLTHLITFAMRQRAAVLLATFILIGVGSWSAIHLPIDAVPDITN
                     PQVQINTAVNALAPEEIEKLVSFPIESEMAGLPQMVELRSLSKFGLSQVTMVFHDDVD
                     LYRTRQLVTERLQTVLDELPPGLSPKLAPVATGLGEIFYYSLDYSADAANKPATREAQ
                     LMALAQIQEYQVKPLLRGTPGVAEVNTSGGYQRQIVIQPDPARLAARGMSLDMLAEIV
                     EQNTRNAGGGYVEIGGEQLIVRATSRVNDIEQILAIPLKFAGGVKPILLSEVATVSIG
                     SAFRTGASTDDGKEALIGATIMLAGENSRLVAQAVRAKLTEIQPKLPQGVIIRALYDR
                     SDLVSRTIATVEKNLAEGALLVVVVLFALLGNFRAAAIVALVIPLSMLFAMTGMVRYH
                     ISGNLMSLGAIDFGLIIDGAVVMVENIVRHLGERQHALGRRLTVSERTEEVLKSAKEV
                     ANPMFFGVLIITVVYVPILALQGIEGKMFKPMALVVMLALGGSLVLAVTLMPVLCSYL
                     LGGKIQEKDNWLVTLTKRLYTPLLKFGLRFKPLIVLPMIVLFGSSLWVFTRLGSEFIP
                     QLDEGDFAFQLIRSSSAGLSSSLDLQKQSEAVIRRDFPEVKEMFSRIGTAEIATDPMN
                     PNVADTYLMLTPRDQWRQENGKPISKEHLGELMRKALLDQVPGQNILVTQPIQMRFNE
                     IMAGARADLVCKIFGDSYDELERLAGEARTIISGIRGGSETEFDNIGKNPMIEIQPDR
                     EAMRKFNVHADELNRLIETALAGTEVGLVIEGNKRSPIIVRLGEDRRSDLEGIKRLAL
                     RTEDGGMLALGQVAKIVLVPQPVQIAREDAQRRVSILINVRGRDTEGFVQEATQAIHD
                     KVKFPDGYYFEFGGQFKNLQEAKARLMIVVPLALALIFVLIFLSFSSFRQATLIFMCV
                     PLAVTGGIFALHLRGMPFTISAAVGFIALSGIAVLNGIMLISYINQLRTEGREVREAV
                     VEGTLTRLRPKLMTALVASLGFVPMAIATGAGAEVQRPIATVVIGGILTSTFLTLLVV
                     PVLYEWIERKTKPPTNTP"
     gene            85935..86372
                     /locus_tag="PLP58_00385"
     CDS             85935..86372
                     /locus_tag="PLP58_00385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ07007.1"
                     /translation="MKTLLNILIIAALATTSAFADAKVKAGPRKGLILDLGGKQAEFF
                     VEKDRSITIAVYDAAMKAQPASTEVITATAEAPSGKVKMEFANKDGKLVSTTKLPEGE
                     GYQVVVQAQAAPDAKKKNFRIKLLLYTCKECSNPEYACTCADH"
     gene            86554..86769
                     /locus_tag="PLP58_00390"
     CDS             86554..86769
                     /locus_tag="PLP58_00390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ07008.1"
                     /translation="MAKPVTMRDVLNKLDELTNALRRGELAAYDHASVQAQDPAKEGE
                     DVLVWGVFAKRSKSPMSTALNRTHFLA"
     gene            86766..88313
                     /locus_tag="PLP58_00395"
     CDS             86766..88313
                     /locus_tag="PLP58_00395"
                     /inference="COORDINATES: protein motif:HMM:NF024753.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HRJ07009.1"
                     /translation="MSRHRFALIAAAGVALCSFALATTVCAQNWPQASGPNLNWKADG
                     ADPPLEWSVVGGKNILWRTTLPEGGQSAVTIWGDRAFVTTHRPMDETSDRLEPNIVGY
                     CLDANTGSILWKVDLPGTDPVQTAGIFSDATVFAPIADGEHVWFFNRCGSMGCFDFDG
                     KPVWLREFRPRTRHTNRECEPILVGDKIITVEVRNKEAAQKLERHKPVPEGIEPRDVW
                     TYLHAIDKKTGKVVWIEEAGTCVHNTPMAGQLPDGRWAIVHGRGGGHGPLEEPYGVSL
                     TLLDSGKTLWSREVKGDSSYNSHWNAKHVFWFQGSNHLVLDTTTGEILRTQDVAKNVN
                     VTDAQTGKRESGIAIKGGKRMPLTNQTNIVVGDWHYFLAHDITAIGRVNIETGRTEYL
                     RVPVQLVAGPEPRRIWEKNDAIPNDTRNSRGIDIALDKRAKGTGWGHVSAASPILVNR
                     HLFFPIMNGTVYVIDAFAETLDEKALIAVNDLGPAGETWTLASFTYANGRLWMHTMKE
                     VLCIGKN"
     gene            88350..90410
                     /locus_tag="PLP58_00400"
     CDS             88350..90410
                     /locus_tag="PLP58_00400"
                     /inference="COORDINATES: protein motif:HMM:NF019259.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1592 domain-containing protein"
                     /protein_id="HRJ07010.1"
                     /translation="MIIRAHTSPISNRISLVSVLICAGFVLPALAAETYTPGEPVRAK
                     FKDFALPFLEQNCFECHDNDIQKGDLNLLELSRVDETNAATWKSVWAQVALEEMPPKK
                     RDQPEVIDRLRFSDWIVGELQRVMKDKGGFHAHLDPKKGNFISHDLLFGPLPEGIQLT
                     PTSSPARIWRVTPQEHITRLNELINTEPKYDPAKPGLRAHGDVVPTNHGGELKLYFGI
                     DNITSVVGGTVAYATAVKSVPVVLSSARKVGLKNYPDFYTVNSAEATQILGKAEDILK
                     YMAYGPLSLVGMPEQITDDPKTYDKVKPKGDLRGLPTAIVYNTKVVRPLTPVLDLMKE
                     PEVTDECLRAAVDYLFEALTFRPPNKAESDSYLQIVKDSIAKVGKKDGVMMGLSSIFL
                     DRDALFRPELVEGGKPDEHGRVMLQDWELGLAVNHALRYIKPDEPLRQAILDARMRTR
                     DDVKREVTRMLDDDSIRKPRVLQFFRDYFDYDQAGYICKDTRALAATGVSTRGESHYR
                     AMFDATASTDRLIELVLAEDKNVLKELLTTQRVVATQTDNTYFGRKHTKEEQAAAVAA
                     KKKAEEELAHSENDEIKALKAEVEAAEGILKSTPDDKAAQKSLDRQKKLLAAAEKRIE
                     NARRERKRGAGPNAEVAEADLTGPPIYARVSRPTFGAGSMKPERILATAPEGQRLGIL
                     THPA"
     gene            90429..90656
                     /locus_tag="PLP58_00405"
                     /pseudo
     CDS             90429..90656
                     /locus_tag="PLP58_00405"
                     /inference="COORDINATES: protein motif:HMM:NF019256.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1588 domain-containing protein"
     gene            90633..90986
                     /locus_tag="PLP58_00410"
     CDS             90633..90986
                     /locus_tag="PLP58_00410"
                     /inference="COORDINATES: protein motif:HMM:NF019253.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1585 domain-containing protein"
                     /protein_id="HRJ07011.1"
                     /translation="MYNHAGIFRTTELDKPVDATGEIIDSGDPALDGPVANALEMIQK
                     LAESERVEQVFVRHAFRFWMGRNETLNDAPVLQAAHRAYRDNGGSMKALITALVFSDA
                     FLYRKVERVAKTAGK"
     gene            91068..91928
                     /gene="nadC"
                     /locus_tag="PLP58_00415"
     CDS             91068..91928
                     /gene="nadC"
                     /locus_tag="PLP58_00415"
                     /EC_number="2.4.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012420303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004514 - nicotinate-nucleotide
                     diphosphorylase (carboxylating) activity [Evidence IEA];
                     GO_process: GO:0019363 - pyridine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylating nicotinate-nucleotide
                     diphosphorylase"
                     /protein_id="HRJ07012.1"
                     /translation="MKLDTATESLISLALEEDVGAGDLTALYFVPEAARSSARVVARE
                     PGVAAGLAVAARVYEKLDPRVSVRALLADGDAFEKGRSLMEIEGPSRSVLTGERTMLN
                     FLQRLCGVAAQTRRHVEAAKPHPVQIWDTRKTTPGWRVLEKAAVKAGGGVNHRMGLYD
                     HVMVKDNHLAAEGGLEALQAAIDRVRSEHPGARVQIEADTLDQVAAFLTLRGVDMLLL
                     DNMSCDDLRGAVRMNAGRLWLEASGGVTLATLPQIAATGVNAISVGALTHSARALDLG
                     LDFDAGGGLR"
     gene            92012..92986
                     /locus_tag="PLP58_00420"
     CDS             92012..92986
                     /locus_tag="PLP58_00420"
                     /inference="COORDINATES: protein motif:HMM:NF024863.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDSL-type esterase/lipase family protein"
                     /protein_id="HRJ07013.1"
                     /translation="MIRQAIFTACATLLACPPIQAQENSAPALPVSAQTKTFEFKDGD
                     RLVLLGNTVIEREQRYATFEPLLALALGERKVSVRNLAWSGDTVFGHARSYFGPPKEG
                     LERLEVHLDLLKPTVVLLCYGSEMAFERLSGLPEFLTGYRNLLEIIRKKSPGVRLIIA
                     APPPLETLPPPLPDQTDANKALSSFRDALRKFAGSQNVFFVDWFELMGGMPKPGVVQS
                     PLTENGVHYTRHGYEKLSAKLIEALGLEVPKISSAELEPMRREVLRKDELFFNRWRPQ
                     NETYLFGFRKHEQGQNAKEIPMFDPLILEADGKIQEIKTGILDARKRL"
     gene            93011..96418
                     /locus_tag="PLP58_00425"
     CDS             93011..96418
                     /locus_tag="PLP58_00425"
                     /inference="COORDINATES: protein motif:HMM:TIGR02604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HRJ07014.1"
                     /translation="MIARILLLSLACCLPLGASQPKSTADLPDPNPEVQKAGFVVPEG
                     LEVTLWAQEPMIQKPVQMNWDAQGRLWVVSSTTYPQIKPGDGTKDQVVVLEDTDGDGK
                     ADKSTVFAENLHIPTAVIPGDGGVYVANSTEVLFLKDTNGDLKEDERTVILSGFGTED
                     THHLLHTMRHGPEGLLYFLQSIYIHSHIETPHGVRRLMGGGVWELRSETRRLEILSKG
                     LINPWGFEFDKWGQSFATDGAGGEGLNYIFPGSVFKSSPGAARTLNGLSPGQPKQCGL
                     EYLDDPHWPEEWRGTWVTNDFRGNRVNRFKLTQSGSGYIAKQEPDVLASNHRAFRPID
                     VRTGPDGALYIADWYNPIIQHGEVDFRDPRRDLVHGRIWRVTAKGRPLSGMPKISGAP
                     VPELLEMLKSERAWTRHFAKRELRERGVAEVMPVFRPWADALPKETGADWHLMLEAAW
                     TREALNRFSAKHWRELYSSPDARIRAAALRILTHRWRELPEALDMLKKAVADENAQVR
                     LWALAVLSEMRQPQAFDAAVRALDMPMDENLDFLLELTAREQASIWLPAYQKEVIKLN
                     ENPRHLVYAMRASGSSEALKPLFDALKEGRLPAEDAAAALALAGDVADAAQAAHMITL
                     AQDPALAAHLSGILDALVKAGTTRGIIPKDAEAAVTAWLADPRAEVVHRATLLAGAWK
                     VENARATLEGILTAETTVPPVREGAMQGLSRLGGVKARDFFDQVFQSHPAHRSLAVQG
                     LAATGPQLAAKRAVEFLASAKTMAEAAPILTTFLNSKQLPAILAKELAGKTIPEPIAL
                     EAVRMVSTRGIQGPLEDALRAAGGVKSMSQSLTPEQMAAMVEKVKTHGDAKRGEDIYR
                     RANLLCQTCHAIGDAGGVLGPNLVSIGGSAPVDYLIESLLDPSAKIKEGYHMIILTTK
                     DGGVISGGLVQDGNEEVIIRDPANQIQKIAKSQIASRQMSPASMMPPGLTASLREDEF
                     VDLVRFMSELGREGEYKIKPNRFVRAWRVMGAMTGEQIDHVRHVGLHALHEKDGGYPW
                     EVRFSKVSGALPLNEVQMAKMYPWFPKIAQISLKLDSPGKVKLGLSSTKAVDVVVGEA
                     ALKEITPELLLDLPAGTHPVTFVIGRDAGELASFSVEILEGAAQVAY"
     gene            97230..98351
                     /locus_tag="PLP58_00430"
     CDS             97230..98351
                     /locus_tag="PLP58_00430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009959619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ldh family oxidoreductase"
                     /protein_id="HRJ07015.1"
                     /translation="MATPYHIVPTAAHNDLVRAAYLHRGYTAAEAEDAARFCEMASAH
                     GIRTHNAIKALHLDHLFGSATGGCKPGAEIRVVEKKFAASEVWDARLKLGQSVAFRAM
                     ERCMELADQYGIGQVSVDNAFHYLWGGGYVMDAAKKGYIAYTNCTSTLAEVVPFGGKF
                     PVLGTNPHSWGLPTQDACGFPVVIDWATSTVAMGRVQQLKREGKPLPPGAAVDAEGNP
                     TTDANAAAWLLPFGAHKGYGLSLLIELMGGMIGGSLPTLRGGGAHPEVKAAGFPAGEK
                     RTSSFYFQVIHPDAISSGMFARGRGFAENLKAVLDDILGHGNEGCMLPGQPEAQNAAH
                     TAKAGGLLFSAAEVEALNEIAAECGSAAFEASALPVYEV"
     gene            98369..99112
                     /locus_tag="PLP58_00435"
     CDS             98369..99112
                     /locus_tag="PLP58_00435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007418595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenyl transferase"
                     /protein_id="HRJ07016.1"
                     /translation="MKLNPMAAPAPAKDIKIVPRHVAIIMDGNGRWAKERGLPRTEGH
                     RQGAETVRRVTEACGDIGVEYLTLYAFSSENWKRPKREVDALMRLLEQFLRKKTPEMR
                     EQNIRLQAIGRLHDLPKPCQEQLHRSIEATSQNSGLTLILALSYGGREEILDGVKSLI
                     ESIERGHLDKGMVDTDVFSKHLYTRYYPDPDLLIRTSGELRLSNFLLWQVSYAEFYIT
                     PKLWPDFDKEDFFEAVREYGRRQRRFGGV"
     gene            complement(99229..99594)
                     /locus_tag="PLP58_00440"
     CDS             complement(99229..99594)
                     /locus_tag="PLP58_00440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009962960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dabb family protein"
                     /protein_id="HRJ07017.1"
                     /translation="MKLLVLLTLLMTATATAALANTPYRHVVFFKFKDSATPEQVAGI
                     ERDFAELAKKIETVEAFEWGTNVSPEGLNDGFTHCFLVTFANKAGLEVYLPHPAHEEF
                     VGKLKPLLDKVCVVDYVAR"
     gene            complement(99682..100131)
                     /locus_tag="PLP58_00445"
     CDS             complement(99682..100131)
                     /locus_tag="PLP58_00445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009960683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HRJ07018.1"
                     /translation="MPDTSARFVQIENAVRETNPWTHNEWLCRPDLVEAEKLLLVRAN
                     MDPGHCHPFHFHPHREEIIYVVYGRAEQWVGSEKRILRAGEMAHIPPGVVHATYNPHD
                     EPLVFLAILSPAKLPDDLAAVPDPQDVSDQAPWADIREGLPVCLTLG"
     gene            100253..101329
                     /gene="mnmA"
                     /locus_tag="PLP58_00450"
     CDS             100253..101329
                     /gene="mnmA"
                     /locus_tag="PLP58_00450"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009963197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HRJ07019.1"
                     /translation="MKILAAMSGGVDSSVAAALLAREGHDVTGAYMKNWINEENVAGH
                     CPWQQDIEDARAVAERIGIGFQVVNLMREYRQRVVKYLLEGYQEGVTPNPDVMCNREM
                     KFGVLWDWARERGFDAIATGHYARKTPDGPGILRGADPNKDQSYFLAMMRPEQARIAR
                     FPIGHLLKPELRALAREMGLQTAEKKDSQGICFIGEVRMEDFLRAFVPDQPGPIVDLQ
                     GRVLGGHKGLHLYTLGQRKGIGVASPVHKQAYVVVAKRPAANELVIAIENEDTPLLWA
                     RRCRVSGLSHVTEPLETPRLFQAQPRYRCPAGAADFTPLGGGRAEIVFHQPQRALTPG
                     QICALYDGERLLGGAVFESVEHGE"
     gene            complement(101347..103389)
                     /locus_tag="PLP58_00455"
     CDS             complement(101347..103389)
                     /locus_tag="PLP58_00455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009960672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetylxylan esterase"
                     /protein_id="HRJ07020.1"
                     /translation="MILPHIFPVAFCLVLATDSLAQKPKSDPAPLPPLNRFPHMVQEW
                     MVGQVRAAEERGNARRAALKTRGDAEAYVQSVRERIRECFGPEPERTPLNARVTRVVE
                     RDAYNIEMVVFESRPGFLVTGNLYVPKGRPVKMPGVIGVCGHSTNGKAAEAYQAFSQG
                     LARMGYVCLIIDPPGQGERFQYLNGFKSRFNPGTGEHIQAGNQQTLVGEFLGSWFAWD
                     GIRALDYLLTRPEVDPAHVGVTGNSGGGTQTTWLCGVEPRWTMAAPACFVTTFRRNAE
                     NELPADTEQCPPRVLALDLDHSDFLAAQAPKPTLILAQEKDYFDARGATEAHQRLKTL
                     YTLLGQPDNTRLHIGGDYHGYSQVNREGMYRFFNLATGVSDAQAEPGLTYEKDETLWC
                     FPEGQAGKASRTLFSFTEEKAARLAQERGPVEGVALQDAIRSVLKIPEAPGSAPDYRI
                     LRATGARQYPAKGYCAYAVETEPGIHALVTRLHDDTLTSRPPLGQKRAVLYLSHRSAD
                     EELRGEPLIQQLLAEEKGAAFYACDLRGIGDSQPDICGINQMLRPYGSDYFISAHSLM
                     LDRPYLGQRVFDALRVIEWLAGQGHEEIHLAGRGWGALAAVFAAVLEPRARQVTLKNG
                     LSSYLEVARTEDYKWPYSMLPPNILAHFDLPDCHAALRDRSLRLLEPWGAADGMNP"
     gene            103605..104120
                     /locus_tag="PLP58_00460"
     CDS             103605..104120
                     /locus_tag="PLP58_00460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ07021.1"
                     /translation="MKNASPLPNAGTHFSGFALVSFGELNVPGGPMEFGFQLDFVPDE
                     IQARQLIFGLLCHSENSPDGEHGFAVRVDLTTGEIWDALNDNGIVGWVENPDKLERFS
                     TEEPLLMSWRVEHLGGALIPKLHIAGEDHLYPSLRYEPGMVMTTVAGTDGGLQSALNG
                     FMHPAVWQELM"
     gene            complement(104179..104748)
                     /locus_tag="PLP58_00465"
     CDS             complement(104179..104748)
                     /locus_tag="PLP58_00465"
                     /inference="COORDINATES: protein motif:HMM:NF013331.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ07022.1"
                     /translation="MKKIIVLLALALCLPLTAAEPGNAANTVCPISGKPVDPSITWEY
                     EGVTYAFAVEACRKKFIEARESSLYHKLGGKAAIDAAVDAFYVKVLADKRVNHFFEDV
                     SMVKQHRKQKEFLSAAFGGPAPWTGKDMRAAHRDLPGLNDEHFNAIAGHLKSTLEDLK
                     VDKALIDQVLAIAESTRADVLNRPKAAKP"
     gene            104860..105264
                     /locus_tag="PLP58_00470"
     CDS             104860..105264
                     /locus_tag="PLP58_00470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009964671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HRJ07023.1"
                     /translation="MQLTYHTDYALRVLIYLISRPERKVTTREMAEFYGVSLNHLTKV
                     AKALTRAGWLVSTRGAGGGLMLAPHTPDTRLGEIVRFTESGDIVECFTPATNTCPIIK
                     SCALKPVLYQARRAFFEVLDAHTVRDLARLEK"
     gene            105413..106267
                     /locus_tag="PLP58_00475"
     CDS             105413..106267
                     /locus_tag="PLP58_00475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ07024.1"
                     /translation="MSLATFNLQAGAPAPKNPVVPVEAVDWKARTISPVTNPIFFEDA
                     VIRSEIRPIFAYHNIHDDFLGGGDAQLYALQIRYALTDRLAFIATQDGYFDINNDAIA
                     NPEGWMDIALGFKYALIDDIESEFILTPGFTFHIPTGERDVFQGRGGGEWNLFVSTQK
                     GYGDLHLSGNLGVRLPNNGSEQSTMLHYSAMVDYHTCRWFIPFITFNAFTVLDEGGNI
                     PLNTEGYDVINFGSSNTRNRTQGALGAGFRSRILDNVDLGFAYEKAVIKPEGLFDDRF
                     TVDICIRF"
     gene            106389..107483
                     /locus_tag="PLP58_00480"
     CDS             106389..107483
                     /locus_tag="PLP58_00480"
                     /inference="COORDINATES: protein motif:HMM:NF014244.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HRJ07025.1"
                     /translation="MRWSFKIATVAGTEVRIHITFFILLAFVALHGMGRGEGLPGALD
                     AVLFISAMFFCVLLHEFGHVFAARGYGIRTPDITLLPIGGVARLERMPRRPAHELVVA
                     VCGPLVNVGIALLIYLALGLRLDADPAAFRFEQSGHFWEKLMLWNLLMVVFNMIPAFP
                     MDGGRVLRALMAMVFDYGKATRWAAAIGQGIALTVVLWMLLAGVFQPILLLIAFFIFM
                     AAGQEAAAVSESEATRGLRVRDAMLTDFHTLPPDAVLADAVDLLISGSQHDFPLLERD
                     GSLLGVLSRQRLIEGLSKHGTLHLAADCADECAESVTPVTSLGEAVERLRSIRCPMLP
                     VLDPVSGRLVGLLTAENVAEALMIRAALRR"
     gene            107676..108911
                     /locus_tag="PLP58_00485"
     CDS             107676..108911
                     /locus_tag="PLP58_00485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ07026.1"
                     /translation="MKTADELMLVPRKEGFQAWRSRNGSGTHPEPENRSRKGADWIAL
                     PARCLVSVPMHFQGVDAARREAAAQLELEAAGFAGETAEAHDFELFPLGNDSRDLPAA
                     AFVQVAPLPETVLDEGRDARFAPSVAFHKLNPGELLIWREDGQLVLAVPHESGQPLHC
                     QALAARHLDDDAAAEIRCVLASLDLAALMPPVQSVAVVLSDGETDESLAAFEAELDLP
                     VVARRETAPVLPGQTSRLIPSQIVRERAERQHRRLVAMGALAFVFVLVAALGAFAVRV
                     GLRDRALAAETARLDGLEPELAAIRDAREAWEDMRTAVNPEHYPVEALYQLVSLLPPE
                     GIRLTRFEVREDGMVLDGEASSLGHGIEFRDKLVGADAFKHWRWDFPQPTNLPDGRAT
                     FRAEARVPDESETDGGESS"
     gene            108908..109432
                     /locus_tag="PLP58_00490"
     CDS             108908..109432
                     /locus_tag="PLP58_00490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ07027.1"
                     /translation="MTASEQRLALGLGAVLVIGGAFIGLTRLKSWKQRVDARAIEVQS
                     RKLEADDLLSQQDFWNTRLQWLEQKQPLYTRRSEADLSLLDLIQKSAGSHGITLTQNQ
                     PVAPSERPGLTSSNMNVEARAGWEAMNRWLHDLQRPESFISIPALTMTPNEEDTSEVI
                     VNMNIQKWFRLPPS"
     gene            109429..110289
                     /locus_tag="PLP58_00495"
     CDS             109429..110289
                     /locus_tag="PLP58_00495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ07028.1"
                     /translation="MKTSAILFLFWAAATAVAQDQPAADDSPAVAMPQAGVSGAPPVP
                     VEIPAEVDSPPVEMSALAREGQPATPGADDPPEEIAGENIPKAHPDSRYQAAWSKNPF
                     MLKTVAVAQATVSFAQDWALAGMYKSTTGKITVTLQNKQTQEYQRVTNEDTEGEFRLV
                     EAKFNRNRNEASVIIAKGSQSAEIKYDDSLISKPVTINNTLRPPANAAGQPGAQQPGM
                     PGAPGVPVSATGGVGGVPRPGVTVPGTTRTLVPGAVGAPGAASAVTPPSISRRRQLIP
                     PPTVAPPPQP"
     gene            110483..110803
                     /locus_tag="PLP58_00500"
     CDS             110483..110803
                     /locus_tag="PLP58_00500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ07029.1"
                     /translation="MPAESWARDWSLAGIYKSTDGVVSVFLKSKTTGMHRQIQNNDAN
                     DRFQIVEARLHRNRDSAQVTLSLDGVTADFGFEFTPAMPAPPSAVAPPSISKRRALIT
                     PPQP"
BASE COUNT        20653 a        34890 c        35050 g        20352 t
ORIGIN      
        1 agaagatcgc catcgaggac acccacgcca ccgacctcca cccgctcgcg aagaagatcc
       61 tcacccactt cgccgaccac gagacgctca cgctcagcca gctcgtcaag ctcacccacg
      121 gcaaacccag cacgctgaag ctgcgtttga aggaactcgt ccagcacggc cacctccagc
      181 cgaaaggtca ggggcgcggg gcgcattatg tgaagaggtt gtagcttgtg aaatcatctg
      241 cccatcttga tcccatgaaa aagtctcgcg atacaaccct cgctctgacc actgactaca
      301 agcggttcgt gaccgatctg aagtcacgca tcgccagcgc acggctggct gcggctcgac
      361 acgtcaatcg tgacctggtc acactctact gggacatcgg gcgcggcatc gtggagaagc
      421 aaaaagaact caactggggc gagtccgtcg tcgaaatggt cgcggcagag ctgcgggcag
      481 cgttccctga catgagtggt ttctcgcctc ggaacctgcg ggacatgaag cggttatatc
      541 ttgcctacag cagtgaagca atttggccac agcttgtggc caaattagaa aatgaaggtc
      601 ccaaggcgaa atttctgcga cagcctgtcg cagaaaaggc atccagcaca aaatggccac
      661 aagctgtggc caaattggga tccaccgagc caggatcatt tctgccacag cttgtggcag
      721 aaattccgtg gggacatcat cgcttccttc tcgacaaggt ttccgccccc gcagcccgcc
      781 tttggtatct gcgagccacg gccagcttcg ggtggtcgcg gaacgtgctt ttgaatcaga
      841 tcaaggccgg tgcctacgag cgggcagtca cggagaagaa gacacacaac ttcgacctcg
      901 cgctgccaga gcactttgcc gagcaggcag aggagatgct caaaagctcc tacaacctcg
      961 agttcctcgg cctgcgtcgt gcggtgcggg agcgtgaact cgaagaccgc ctcatcagtc
     1021 gcttgcaggc cttccttttg gaactcggct acggcttctg cttcgtcgga cgacagcatc
     1081 ggctcgtgct cggaaagaag gagtatttca tcgacctgct cttctaccat cgcttcctca
     1141 aagccctcgt ggccttcgag ttgaagctcg gccccttcga gccggagttc gcgggcaaga
     1201 tggactttta cctcaacctc ctcaacgaca ccgaacgcgc ccccggtgac caaccctcca
     1261 tcggcatcat cctctgcgcg gaaaaagatg acgtggaggt cgagtatgcc ctccgcacca
     1321 aagccaaccc catcggcgtc gctgcctaca ccttgcgatc gaagctgccg ggtgagttga
     1381 agggcagact gccgacggcg aaacaactgg cggatattgt tcgctcggaa atggagggtg
     1441 ggaaatgagt ggaattccaa gcacatcccc caagagcagc gcacagtcct cgtattcaag
     1501 attatccatg cgccagatca tccaaatcat cttctgtgtt ctgttagttc agaacaacgc
     1561 cttggccacc acatacatcc tgccgacctg ggaagaatcg tccacaactt cggagtttgt
     1621 tggcgtcgtt gagtgcatcg ttgctggagg cattgtagcc cggtatcgcg ttgtggattc
     1681 atggaaaggg gctgccgttg gcactgagct tcacatcgcc cagcacgttg acccctatgg
     1741 ccctcagttc ccaatttctt tggtgggcga aagatcactc gtctttgcgg aaaagtcccc
     1801 gacctatcgg cttgggtctt tcagtggggg cggtgccact ccgctctggt ggcgcaagat
     1861 tcctaccgac ttaaggtgct ttagcgtggt gccgatcaag gagccgatgg ggaggtgtct
     1921 ctccatttac atgggcttta gaggtggcgg tcttgctcag ttcaaagcgg aagtcaccag
     1981 tttccttggg gctgacagtg aaggccaaga gatgcggctg atgctcgctg ccgctcgaaa
     2041 gaatctgcat cttccagatc cgactgaacc cagcgacaag ggcaacgagg aggacatcaa
     2101 actttacgag tggctcagtg gagcaaagga cattgagcag ctatggcagc gcattcttgc
     2161 gcaagctaag caacttaatc cgccccccat tcccaaaacg gcggaggaaa tcaaggctga
     2221 cagccataag cgcatattgc tggagatgat cagcagtggt ggacgagagc gatgcaaggc
     2281 tttgttggaa ctggcagatg tatccaccct accgtgggac aagaaagagc tcgacagagc
     2341 cgcatcctac attcgccgga gcctctcgcg tgctgaagga cggatcattc cttcgcaaga
     2401 cagcgaacag aacaagccgc ccgccactgc cgagcaaatt gcaaaagccg agtctgcgct
     2461 ttcgaagcca tgggatggtg aaactggtaa ggctttcgaa cttctttgtc accactcccc
     2521 aagctctttg gtgccattct tgaacggctg gcagcctaca aaagaccgaa ggaatcaaca
     2581 gtttggatac acacttggct ctgtctttgg gcatctttgc gcttcggatc gaatccagca
     2641 tctaagctca ctgctagcgg caaatgacga ttggatcaaa gtttctggag ctgtctatct
     2701 ctgctttgat gatgccaaag caggcaagga ggcattgagc agacttttaa gccttcaagc
     2761 agatgctgga gcatgggcag ccatcgtttt agcaagcaga ggcgagaagg ccgccatgcc
     2821 cagagccctc gaagtgatgg cgactcccga tgacccaagc atcttttcga tcaaccatca
     2881 caacctgcaa ctacgcctcc gagtgctgct ctcgaatgtg gcaagtgctt ctagcatcag
     2941 ccagccacca ggtccgtcca aagaatactc tgatgggcct gaacgcttga tcatccaaaa
     3001 ggagtggcac gaatccctct ccaaatggtg ggagatgaat caggataaga tcaagctcgc
     3061 ggacccctgg gccaagtttt tggacgaaca gagagtggac tgatgcccca cagccgggac
     3121 ggctgcacca cattggctcc ctgtgcccgt ttgctcgcct tgcgatggca atcttgccaa
     3181 gacgtggcgg gctggacgcg gcagaatcag gctggtgaaa tcggttctga cactctttgc
     3241 gatctctgcg ttctttggcg gccatgcttt ggcggcggag agcaggccca acatgctctt
     3301 catcgcgctg gatgatctca atgactggat tggctgcctg ggcgggcatc cgcagacgag
     3361 gacgccgaat ctggatcggc tggcggcgag cggagttttg tttcggaatg cctacacggc
     3421 ggcggcttcg tgcaatccat cgcgcacggc gatcttcagc gggctggctc cgcatcgctc
     3481 ggggctgtat cataacctgc aaaagatgcg ggaggtgatg ccggaggcgg agctgttgcc
     3541 gcggtatttt tcgaggcagg gttactggag cgcgggatcg ggcaagatgc tgcattacat
     3601 catcgatccg ccgtcgtggg atgactactt tccggccaga gcgaaggaca atcccttccc
     3661 gcacacgttt tatcccgaga aacggcccgt gaacctgccg cgtgccggtg actggcaata
     3721 cgtcgagacc gactgggcgg cgctggatgt cacggatgag cagtttggcg gcgattgggc
     3781 cgtgacgaag tggattggcg agcaactcca gcgtccacat gacaagccgt tcttcctcgc
     3841 ctgcggcatc taccggccgc atgagccgtg gtttgtgccg aagaagtatt tcgagccgtt
     3901 tccgctcgaa agcatccagc ttccgcccgg ctacaaagcg gatgatgtcg atgatctgcc
     3961 acccgcaggc cagcgactcg cgcggaatcg ctactttgcg cacattcaga agcacgagca
     4021 gtggaagaag ggcatccagg cctatctcgc gtcgattcac ttcgcggatg cgatgctcgg
     4081 acgcgtgctg gatgcgctcg aagagagtcc gcagcgcgac aacaccatcg tcgtgctctg
     4141 gagcgatcac ggctggcacc tcggcgagaa ggagcactgg caaaagttca ccggctggcg
     4201 tgtgtgtgcc cgcgtgccgc tcatgattcg cgtcccgaaa ggggcgcccg gtttgcccga
     4261 aggcaccacg cccggcagcg tgtgtgatcg tcctgtgagc ctcgtcgatc tgtttggcac
     4321 gctcaccgat ctgtgtggcc tgccgccgaa agcggacatc gaaagccgca gcctcgcacc
     4381 gctcctgcgc aacccgcagg ccgactggcc ccacgccgcc ctcacgcatc tcgacaagcc
     4441 cgagaactac gcgctcagca cgcaacgctg gcgctacatc cactacacgg gcgacgaaga
     4501 ggagctctac gacctcgaaa ccgatccgca cgaatggacc aacctcgcca caaagcccga
     4561 gcatgcgaac aaactcgctg agatgcgttt gcttgctcca aagaacatcg cgcctgtcca
     4621 caaatcgaag ccatgaaagc cattgtcctc acctcactcc tcatggcggt gcatttgcac
     4681 gccaaaccac tccaagtctt catcctcgcg gggcagtcca acatgcaggg ccatgccaaa
     4741 gtgagcacct ttgagcacat cggcatggat ccggccacga agccgatgct ggatgaaatg
     4801 atgggcgcag atggtaaatc gacgatttgc gaacgcgtgt ggatttcgtc catcggttgt
     4861 gcgggtgata acacgacgga gcagaccggc aagctcacgg ccggatttgg cgcgacgaag
     4921 gagaaaatcg gtcctgagct cacttttggc cttacgatgg agaaactgat cgacgcgccc
     4981 attttgctca tcaagacatc ttggggaggc aagagcatca acaccgattt tcgcccgccg
     5041 agcgccgggc cttacgtctt caatgaagcc cagctcgcca cgatgcagaa gcagggcaag
     5101 gacatcgccg cgctcaaagc agcgaaggaa aaggaagccg gcgtgtatta ccggctgatg
     5161 atcgagcatg tgaagaaggt gctcgctgac atcaagcgcg tggttccaag ctacgatgcc
     5221 tcacaaggct acgaactcgc tggtttcgcg tggttccagg gctggaacga catggtggac
     5281 aacggcacct atccgactcg cgacaaaccc ggcggctatg acgactacag caaagtcatg
     5341 gcgcacttca tccgtgatgt gcgcaaggat ttggacacgc cgaagctgcc cttcgtcatc
     5401 ggcgtgatgg gtgtcggcgg cccaacatcg gagtatggac ccaacgagca gcgctacaaa
     5461 gccacgcatc aaaacttccg cgatgccatg gccgctccgg cggagttgcc tgagttcaaa
     5521 ggcaatgtcg tggccattcg caccgagaag tattgggatc gcgaactcaa actcgctcgc
     5581 gcgaaagaga acgaggtcaa agacaaggcg aagaagctcg ccaaggagca aaagctgaag
     5641 cctgcggagg aaagagccct cgcggacaaa atgcgtgctg attcgctcac cgagcgtgag
     5701 cgcctcgtgc tcgaaaaagg cgtctccaac gccgagttcc actacctcgg ctccgccaag
     5761 atactcggcg gcatcggcaa agggctcgcc gaggcgatgg ctgaactgaa ccacctcaag
     5821 aatacggtcc gatgagaatc gccgctctca ttttgttcgc tctcgccggc ctttgcagcg
     5881 ccaatgcaaa acccaacatc gtcgtcatca tggctgacga tctcggttgg agggatctgc
     5941 actgctacgg caatgcatcg gtggacactc ccaccctgga tcaactggcc gctgacggca
     6001 tgcgtttcac cgacgcctac gcggcttctc ctgtttgcac gcccactcgt gccgccatga
     6061 tgaccggcca gtcgcctgcc aggctccgca tcaccaacca tgcccccggc aatcccgatg
     6121 gttttgcgct agaaggctcg tccctgcacg aggccccaaa catccgccac ctgccgcttg
     6181 ccgctgtgac gatcgctgag cggctttccg cggcaggtta cgccacggcg catgtgggaa
     6241 agtggcacct ttcccatgtg gtccggggag acaaacgcct ggcagaaacc gacctccgtc
     6301 ctgaaaagca aggttttgac ctcaatgctg gcggcaactc cagcggcgga ccggccagct
     6361 actttgcccc ctatcgcaat tccgcgttca aagacggaca ggacggtgaa tatcttccag
     6421 atcggctcgc tgacgaagcc atccgcttca ttcgtcagca caaggagcag tctttttatc
     6481 tcaactggtg gccctactct gtgcattacc ccatgcaggc acgggaagca ctgatcgcca
     6541 aataccgcca gagaactgag atcaaagacc cgatctacgc cgcgatgatt gaaggcctgg
     6601 acacagccat cggcagcttc ctcaaggccc tcgacgaagc tggattgcgc gagaacaccc
     6661 tcgtcctctt caagtcggac aacggtggat atcatggcga caaccgcccg cttcgtggat
     6721 tcaagggcat gctctacgag ggcggcatcc ggataccatg gatcgttcgc tggcccggtc
     6781 aggttccgcc tggaaccctc aattccacgc ccgtgatcag cacggattgc tatccgacac
     6841 tgctcgaggt ggccggcctg cctccgaccg aggcccatcc tgtcgatggc aaaagtctgc
     6901 tgccgctgct cacgcaaaca tccggtttcc agcgggaaac cctttacttt cattatccca
     6961 actacgcctt tcacaaacgc aaccggctcg gcagcgccct ccgcgaaggc cgctacaagc
     7021 tcatccaaaa ctacgacgac cactccctgg agctttacga tctgggcagc gacatcggcg
     7081 agaagaacaa ccttgccgcc caggctcccg acctcactcg acgactggcc gaaaagcttc
     7141 aggcctggct cgtgaagagc gaggctcaac tgccggccag gcactgaata gctcacccca
     7201 tgaaactcat ctcctcattc actctcgctc tctcggtgct tttcgcgtgg ccggcgctcg
     7261 ctgccaagcc gggtgagact ttgacaccgc ccgacttcac gaaaggcggc aggcccgatg
     7321 aggcacacga ctggacgctc gggccgacgg gcgcgcgtgg ctggattttc acatcgcgtg
     7381 gtcattcgcg cgatgcgcgg caaatcctcg tgaccaaaat cgcgccggga tcgcctgctg
     7441 atggcgtgct ggcggtgggt gatgtgatcc tcggtgtgaa cgcagaacct ttcgccgatg
     7501 atgcgcgggt gctttttgca aaggccatca cggcggcgga ggcggagacg ggtgcgttgc
     7561 gtctgctgcg ctggcgtgct ggaaagacgg acaccgtgga gctgaagctc gcggtgctcg
     7621 gcggctacag cgcgaacgcg ccgtatgact gcgcgaagtc gggacgcatc tttgaactgg
     7681 gctgcgcctc gctcgcgaaa cgcatggcgg acgagaagaa ctacacgaag cagctcaacc
     7741 cgatcccgcg tgcgctcaat gcgctggccc tgctcgccag cggcgacaaa gaaaacctgc
     7801 cgctcgtgaa acgcgaggcg cagtgggcgg cggacttcac cacggacggc tatctctcct
     7861 ggtattacgg cttcttgatg atgttcgtca gcgaatacgt catggccacc ggcgacactt
     7921 cagtcatgcc cggcctcacg cggttggcgc tcgaaacggc gcgcggccag agcggtgtgg
     7981 gcacctgggg gcaccgcttc gccaccagtg gcgggaatct cagcggctac ggcgcgatga
     8041 acgcgcccgg actaccgcta accatcggca tggtgctcgc gcgcgaggcc ggcgtgtcgc
     8101 atcctgatct cgatcgtgcg atcacgaagg cggcgggttt cctgcgttgg ttcgtccaca
     8161 aaggcgcggt gccctatggc gatcacctgc cctttcccgc gcatgaggac aacggcaaat
     8221 gcgctgctgc cgccgtgctc tttgatttgc tcggcgaccg cgaggcggcg gagtttttcg
     8281 ccaaaatggc cgccgcggcc tacagcgagc gcgagcgcgg ccacacgggc aactacttca
     8341 acatcctctg ggccatgcag ggtgtcgcgc gctgcggcag cctcaccagc ggagcgtatt
     8401 ggcaggagca gtcgtggtat tacgatctcg tccgcacctg ggacacggcc tgggtctatc
     8461 aaggctcgcc cgtcggcgcg gaggaacacg gcgcctacaa aggctgggac accagcggca
     8521 gtcacctgct cgactacgcg ctgccactga aaagtctgcg cctcaccggc cgcaaagcct
     8581 tcaccatgcc gccgctgaat gccgcgcaga ccgcagcggt catcgccgcc gggcgcgact
     8641 tcgccttcaa agacgatgcg aaccgctacg agcagcgcac gagcgagcaa ctcctcgcag
     8701 ggctcgccag ttggtcccca tttgtgcgca aacgcagcgc tgagtccttg ggcaagcgtc
     8761 aaggcgactt tgttcctgcg ttgctcaaac ttctcgcgag cacggaccgc gatacccgtt
     8821 acggagcggt ggaagcgctc ggcgctctcg gccagcgtgc cgatgccgcc gcgccgcagc
     8881 tccgcgccgc gctcaaggac actgacccgt ggctgcaatc cctcgccgcc gaggccatcc
     8941 ccacgctcgg ccctgccgcg cgcaaaacca gcgtcagtga cctgctcgcg atgaccgttc
     9001 gcacgaatcc cgccgacccg cgtcgtctcg ccgcgcgctc cgccagcgtg gcgctgtttg
     9061 cgaactatcc cggccagcgc ggaccgcgca gcatcctcgc ggattcgctc gatggtgtgg
     9121 accgcgcgca gctttacccc gccatccgcg ccctgcttca gcatgaggac agcatcccgc
     9181 gcggctcggt gagcaaggcg ctcgaccgtc tcaccgatgc cgacctcgtc gaactgctgc
     9241 ccgacatcgc caaagccatc gaacacatcg cgccgagcaa tgagatgttc ggcgatggca
     9301 tccgtctcgc cggcctcgat ctgctctcgc gcctgcacat tcgcgaaggc atggcgctgt
     9361 gcctctccgt catggaaccc gagcgctggg gtgagaaaaa ccgctcccac gcctgcctca
     9421 ccgcgctgca acgctacggc acgcatgcta aagcgatgct gccaaagctg caagtcgtgc
     9481 gcgagcaact cgtcaccgcc cgcagatcga aagagcacct cgccgagttc gacaaagccg
     9541 tcgccgccat tcaatcgagc accgaaacgc ccacgctggt ggacttgcgt gccttccagg
     9601 ctcgtgcggt gaactgaatc catcccatga cacccgtctc catcttcaac gacgtcatcg
     9661 gccccgtgat gcgcggcccg tccagctcac actgcgccgc cgcactgcgc atcggacgcc
     9721 tggcgcttga tctcatggat ggcgaaatca cggaagtgct cgtcgagttc gaccgcgccg
     9781 gttcgctgcc aacaacgcac gaaagccagg gcagcgacat gggcctcttc ggcggcttgc
     9841 tcggctggaa cgccgatgac gagcgactgc cgaactccgc ccaagccctc gccgatgctg
     9901 gaatcaagct tcacatcgaa accgtcgatg tcggcgatcc gcatccaaac acctaccgcc
     9961 tcacgctgaa gaacccgcgc gaaacgcaca ccctcatcgc catctccacc ggcggcggca
    10021 tgatcgaagt catcgagatt gacggcgtgc cgctgtgcat ggacggcggg tatcacgaga
    10081 cactgatctg gctttcaaaa tgtgatgcag acactcttgt ctgcattctg ggcttttcag
    10141 aagcagacaa gagtgtctgc atcactcacg atttgggcga cacgcaactc atccagatca
    10201 aatcatctca atttgcagca acgtccgatt tgaagccaat ctcggtcaaa aagctctctc
    10261 ccgtcctccc cgtcccatct cgcaaagatc tccacgtccc cttcacaagc tgcgcagcca
    10321 tgcttcagca cgacggagac caaaacacgc ctctgtggca actcgccatc gaatacgagc
    10381 gtgcccgtgg gaacttcacc aagcgcgaag tcatcgacaa gatgaccggc atcgtccgca
    10441 tcctgcgcaa atccatcgcc agtggcattg cgggcacgag ttatgaagac cgcgtgctgc
    10501 atcatcagtg cggacgtttc gccgaaatga tgaacacggg ccgcctgctt gatggcggcg
    10561 cgttgaaccg catcatcctc tacgtcaccg ccttgatgga ggtgaaaagc agcatgggcg
    10621 tcatcgtcgc cgcgccgacc gctggcgcat gcgctgccct gcccgcagcc gtcatcgcca
    10681 tggccgaggc gatgaatcaa gatgaggagc aaatggccaa agccatgctc gccgccggaa
    10741 tgatcggcac cttcatcgcc acgcgctgga ccttcgcggc ggaagtcggc ggctgtcagg
    10801 ccgagggtgg tgccgccgcc agcatggccg ccgccgcgct cgtcacgcac gccggaggca
    10861 cacgcgatca ggccatcgcc gcggcctcga tggcgtttca gagcatgctc ggcctcattt
    10921 gcgatcccat cgcgaaccgc gtcgaggccc cgtgcctcgg aaaaaacgtc atggccgcca
    10981 gcaacgcgct ctcgtgcgca aacatggccc tcgccggcta cgatccgctc atcccgctcg
    11041 acgaggtcat cgacgccgcc aaagccgtcg ccgagcgcat gccccgcgaa caccgctgca
    11101 cctccctcgg cggtctcgcc gtcaccccca cctccctcga aatcgaaaaa cgcctcgcca
    11161 gcttcaagaa aggctgcggc acctgcgcct gtcattgaat catccgccca aattttgccc
    11221 gcttcacagc gctggggatt gatcgagcta tgccatgcct cccgctccat gcggtggatt
    11281 ggcatgaatt tgcatgaagc attcgaaaga ggctgacttg ccagaaatgc agttgcccat
    11341 cgcttcgttc agtagccgat tatgctccgt ctattttctc tcattgtccc cgcgttctgt
    11401 ttgctggcag ggtttgccac gtctggtttc gcgaaggatc agcccaacat catttatttt
    11461 ctcgccgatg acctgggctg gacggatgtc ggctggcacg ggagcgagat ccgtacgccc
    11521 aacctcgaca agctcgccaa cggcggtgcg aaactggagc agttttacgt gctgccggtc
    11581 tgctcgccca cgcgcgcggc gttgatgact ggccgttacc cgatccgcca cggcttgcag
    11641 gtcagcgtgg tccgtccttg ggcgcaatac ggtctgccgc ttgaggaacg cacgcttccg
    11701 caggcgctca aggaagccgg ttacaccacg cacatcagcg gcaaatggca tctcggacat
    11761 ttcctgccgg actacctgcc cacgcggcgc ggcttcgaca cgcaatacgg ccattacaac
    11821 ggcgcgctcg actacttcac gcatgaccgc gacggcggcc acgactggca tcgcaacgac
    11881 aaggacagcc gcgacgaggg ctacacgacg aatttgatcg gcgacgaagc cgtgcgcgtg
    11941 gtcgagcagg cgcagccggg aaaaccgttc ttcctttatg tgccgttcaa cgcgccgcac
    12001 tcgcctctgc aggcgccgga cgaatacctg aaacgccacg cgcacctcgc cgacaggaag
    12061 cgccaggcct ttgccgcgat ggtcgagtgc ttggatgagc aggtgggccg cgtggtggcc
    12121 gcgttggaga aacgcgggct ggtgaaggac acgctcattc tgttctccag cgacaacggc
    12181 ggccctctcg gcctcggcgc gaccaatggc agtctccgcg cgcagaaggg cacgctttac
    12241 gaaggcggca cgcgcgtctg cgcctttgcg aattgggccg ggcgcatcaa ggccggaacc
    12301 gttgtcaacg agcctctgca tatcgtggac tggttcccca cgctagtgaa actcaccggc
    12361 gcgcccgccg cgcagaaaac cccgcttgac ggtctagaca tctggccggt gattaccgca
    12421 ggcaaaccct cgccgcatga ggacatcctc atcaacgcca cgcccggcag cggcgcgatc
    12481 cgcatgggtg aatggaagct cgtgctcaac gggcgcattg acgccaacga cctgcaaaac
    12541 gccggccctc cgaatgcgga agcaaaggcg gcccccaaga aaaagaaggc gcaggaaaaa
    12601 gccgctggtg ttgcagtgga acttttcaag ttggcggatg atccttacga gcggaacaac
    12661 ctcgccgggc aacaccccga ggtcgtgcag caactgcgtg caagaatcga ggcctaccag
    12721 aaggccgccg ttccgcccaa ggccgggaac cagcccgcaa actacaaggc cccgcgcgtg
    12781 tggggtgaat aaagagcggt ggcatccaac gctgggtctc gcacagcgag atggttggct
    12841 cggcagctgc ccggtcaaga atccggttgg catacgcgaa ctgcaggcga ggatcctcaa
    12901 ccaacccggc attatccgca tcgcttccgc ttcacttgtc aaggcaccgg cacccgtctg
    12961 ttcggagtgg cacacgagcg caaggtgccg aaggcactct tggcgtatgt ttgattgccg
    13021 caggagccgc cgttaaactc tgattatgag attccccacc ttcctgctct tcgtcaccag
    13081 cgcctccgcc gcgccggttg acttcgtgcg cgatgtgcgg cccatctttg aaaggcactg
    13141 ccttgagtgc catggtgaga aaaaacagaa gtcaggcctc cgcctcgaca tcaaatcgga
    13201 agccttcaaa ggcggtgaca accacggccc cgacattgta cccggaaagg ccaaagacag
    13261 cccgctcatc cgcttcctga cgacagatga cgatgacgaa ctcatgccac cgaaggggag
    13321 actgcctgcg gttgacatcg aaacgatcac gaactggatc aatgaaggag ctggctggcc
    13381 cgagggtgcc gatctcgcca aactcgaaga ccgactcgac cactggagct tcaagcctct
    13441 gctggaattc aacccgcggc acacaattga tgcgttcatt gacgccaagc tctccaaaaa
    13501 aggactccac cgctccccgc ccgccgatcc cgtcacctgg ctgcgccgtg tgacttttga
    13561 cctcactggg ctgccgccga cgccggagga cgtgatcgca tttgagtcgg acttgtccga
    13621 ctcgtccgac cagtcggacg catatgaggc ggttgtggcg cgcctgttaa atagcccccg
    13681 ctacggcgag cgctgggcgc agcactggct ggatgtcgtg cgctacgcgg acacgcacgg
    13741 ctttgaggtg aacaccgagc gcccgaatgc ctggccctac cgcgactaca tcatccgcgc
    13801 cttcaacaac gacacgccgt atgatcgctt catccaggag cagatcgtcg gcgatgcgat
    13861 gggtgaggac gctgccacgg gcttcctcgt gaccgcgtcc gtgctgctgc ctggccagat
    13921 cggcaaagac gagccttcaa agcgcctcgc tcggcaggat tccatcgacg agatcgtcgt
    13981 gaacattggc cagacttttc tcggcctcag cattggctgc gcacgctgcc acgatcacaa
    14041 attcgatccg atcagcgcaa aggattacta ctccatgcag gccttcgtcg ccggagtcga
    14101 gtatggcgag cgcgaactgc gcacgacgga ggccatcgcg gcgcgagagg agctgaagaa
    14161 agtcaaagcg cagctcaccg acatcgagac gcagctcgcg cgcttcgttc cgctcgccaa
    14221 atcgggcgtc aagcgtccga tgattgatgc gcgtgagaat cgcgaccgct tcatgcctgt
    14281 gaaggccaga cgcgtgcgct tcaccatccg cgacacgaac aaactcgaac cctgcattga
    14341 cgaactcgag gttttcacca ccgcaggcga aaacatcgcg ctgaacgccc ccatcaccgt
    14401 ttccggcgag aaagtcagcg ccgaccgcca tgagcagcgt ttcgtgaacg acggcaacta
    14461 cggcaactcg cgcagttgga tgtcgaatga agtcggcaaa ggctgggtga tgatagagct
    14521 cccgcgggag caggagattg atcgtgttgt ctggggtcgt gaccgtcagg ggaagttcaa
    14581 ggaccggctc gccacgagct acgtcatcga aaccgccgac aattccggca tgtggcgcgt
    14641 cgtcgcggat tccgacgacc gccagcgctt tgacgagcga gatcgcacgc agcccgcgtt
    14701 ttccacacag ggtctccgca cagaggaaat cgccgaggca cagagtctca tggatcaaaa
    14761 gcgtccgctc gacgagcgcc tgcaacgcct gagcgccgat caaaaagtct tcgccggcac
    14821 ctttcgcaca ccggatgaca tccatctgct caatcgtggc gatcccgagc agccgcaggg
    14881 gtccgtggtg cccgccgtgc tctctgcgct gggaaaggtg acgctgacaa gggaaactgc
    14941 cgagcaggaa cgccgccgcg ctctcgccga atggatcacc agccctcaga atccgctcac
    15001 ggcccgcgtg atggtgaatc gcatctggca gggccacttc ggcaccggct tggtcgaaac
    15061 accaagcgat ttcggtcgca tgggcatcaa gcccacgcat cccgaactgc tcgactggct
    15121 cgccgccgag ttcatcaaga gcggttggag tgtgaagcac atgcaccgga tcattttgat
    15181 gagtgagacc tatcgtcaat cgtctgtcgt gtcggaccag tccgactcat ccgacgcact
    15241 ggcaaaagac gccaacgccc gcctcctctg gcgcttccct tcccgccggc tcgaagccga
    15301 atccatccgc gactcaatgc tcgccgtcag cggtctgctg gatttgaaaa tgcatggccg
    15361 cggctacaac ctcttcgaca aacgcggcgg tctcagcggc ttcgaacccg tcgagacact
    15421 cacgcccgac aacaaacgcc gcatgatcta cgcgcacaaa gtccgccgcg aacccgaagc
    15481 cgtgtttggc gcgttcgact gtcccgacgc cggtcaaagc acagccatcc gccgcgccag
    15541 caccacgccg atccaggcgc tcaacctctt caacagccgc ttcacgctcg acaccgcgaa
    15601 cgccttcgcc gctcgcgtgc aaaaagaagt gggtggcgaa accgccgcgc agatcaccca
    15661 ggcttaccag cttgctttga atcgcgttcc gaccgccgca gaactgcaag gctctgaacc
    15721 tgccgtccgc gaaaacggtc tcagtgttct ctgccgcgcg ttgttcaact cgaacgagtt
    15781 tctcttcctg ccatgagcga atccctcacg cctgccggcc atcatctgct caaccgccgc
    15841 cagttcctcg caaacagcgc cacggcccta ggctcgatcg ctttgacgcg cctgcttgct
    15901 gcggatggct tgttgatcga tccagcgcgt ccgtatgccc ctcgacagcc gcattttccc
    15961 gcgaaggcga agaacgtggt cgtcatcttc tgcgcaggcg cggtgagcca gctcgaaacg
    16021 tgggactaca agcctgaact tatcaaatgg gacgacaaac cgctgcccgg cggtccggcg
    16081 gtgacgtttc aaggccccgc aggcaatctc gcaagaccgc aatacgcctt ccgtcctcgc
    16141 ggacagaccg gcaaatgggt cagcaacatg atcccgcatc tcgcggagct gacggacgac
    16201 atcgccttcg tgcattcgct caccagcaag tcgaacacgc acggccctgc ggagaatttg
    16261 ctctccactg gcttcaccct cgatggtttt ccctctctcg gctcgtgggt gacgtatgcg
    16321 ctcggcagcg aaaaccagaa tctgcccagc tacgtcgcca ttcccgatcc acgcggcgtg
    16381 ccgcagaatg gcgccaacaa ctggggcccc ggttttctcc ccgccgcctt ccaaggcacc
    16441 gcgatgagtt cgaagagccc cgtgcgacat ctttccgcgc ctggtgtgtc cgcgcaggcg
    16501 gatcaagccg cccgctcgtt gctgcaaaag atgaacgcga ggcatctcga agcgaacccg
    16561 ggcgatcaaa agctcgccgc acgcatcgcc agctacgaac tcgccgcgcg catgcagctc
    16621 agcgtgcccg agatcaacga cctcagcacc gagcccgcgc acattctcaa aatgtatggc
    16681 gcggacgaca cgcagaacgc cgacaaggcc gccttcgcga agaactgcat cctcgcccgc
    16741 cgtctcatcg agcgtggtgt gcgctgcgtg cagcttttca acggcgccta tgccagcggc
    16801 ggcaagctca actgggacgg ccacagcgac ctcaaatcgc agtatgatgt tcacgcctcc
    16861 atcctcgacc agcccgccgc cgcattgatc cgcgacctca agcaacgcgg cctgctccag
    16921 gacacgctcg tcgtctggtg taccgagttc ggccgcatgc ccttcttcca aaaaggcgcg
    16981 aagggccgtg atcacaaccc cgacggcttc acctgctgga tgaccggagc gggcgtgaag
    17041 cccggcgtca gccacggtga gtgcgacgag ctcggccaga aggcggtgaa ggacattcat
    17101 ccgctctacg acttcaacgc caccattctg caccttctcg gcctcgacca cgaactcctc
    17161 accttcgagc acaacggcgt ccggcgccgc ctcacgaacg tggaaggccg tgtgatcaaa
    17221 gagatggtgg tgtgactcca ctcatgcgac cagctgtcct ttgcgcttcg agattgaaat
    17281 cgtcgccttg gttccggagt tgaactcgat aaagtcttgc tcgatgccgt cgctgaaaat
    17341 gacgccgttc tcgcccatca gcgattcgat cacaagcggc tgctgcggcg tcaccttgcc
    17401 gaaaaccagc tccgcggcgg ttgtgttgct cgggaaaggc tcgcgcacgg tgaagaagag
    17461 gtgattcgat tcccacggga aactcgtgtc ctttggctcg gtggccgccg cacggcctga
    17521 ggcaaccgcc actgccatcg aacccgccat caggcttttg aaccagcccg tggaaccgag
    17581 gccggtcgag acgatcacac cgctggatga atggttctcc gccgtattgc ccagcgcgat
    17641 gcgataccgc gccgatccgt gggatttcat gccgatgaag agatcgttca ccgcgcacag
    17701 gctcagtccc gtgttcaatt tcgcctccgc catcgttacc tcgcgttttg accgtccgcc
    17761 gcgcagcacc tcgcgcatca ccttgtcgag ttgcggcacc tggaagggca gaagctgccc
    17821 gtcgtagcgt ttcgggtcag gattcacgcc cacgagcggc tggccgttga gatacttcaa
    17881 ggtgtttgcc accaatccgt cctgacccag cgccaccacg atgtcctgct ctccaaacac
    17941 gaaattgggc agaaaagccc gctgcacgag ctgtacgcgc cccagttcac cgagcgtgcg
    18001 ttgcgcttcg gagatcgcct gctggtaggc ggcgtcctcg cgctcgtaat caccaaaatc
    18061 accgcccagc cggctcacat aaaacttcgc ctgcgatttc gtcgcgaact tcgccttcaa
    18121 atccgccagc cgcgtcgcgc gcacgacgag gatgatcttc gtttcagtca ggcggttcat
    18181 tttaagccgg aaaggtcatt tcttcccgcc cggattcgcc agcagctcgc tcagcagctc
    18241 cggcgagatg ttcagattgc cgatcttgtc cgcttttgcc gccagttctt ggaaagcaaa
    18301 cgcgatcaac tgctgcggcc tcatgccgct ggttgccaac gcttgtagca cggacgcgtc
    18361 cgtgctggcg agggccttca tcgtggcctc gatgccatag gcgcgcgtgt cggactccgc
    18421 ttttgcgttc tccgccgcca gcgacaccag cttcttgcgc tcgtcctcga gaccgatgct
    18481 cgattccagc ttcgcgcgct ccagttgcgc ctctttttcc tgcaccgcct tctccgtgtc
    18541 catctgcgtc tcgcggattt gccgtttctt gttctccacg gcgatttcgg tgttcagctc
    18601 gttctccttg atcgcgcgct cctgctccac ggcggcattg cgccgcatga agatggcctc
    18661 gtccgcgccc ttgagcagtt gctcgcgtgt ttccgcctcc aaagcacgcg ccgtctccgg
    18721 tgtcggcttg atcgccagca gcgaaaggcc cagcacttcg agccccagag ccgccagctc
    18781 cgccgaggtg ccgagttgag cgcgaatcgc cgtcaccaac gtctccgaag cgcgcaatgc
    18841 ctgccgcagc ggcagtttct cgatctccgc gcgcgccagc acatgcacga gattgatcac
    18901 gcgttgtggc agcttttctg gatcttcgga ggcgtagcct cgaccgtgcg gtgccagcgt
    18961 gaagtccatc aacgccgcca gtttcttcgg atccgcgatg cggtaggtga tctgcccttg
    19021 caggctcacg ctttgataat ccgccgtctg ctcctcaaag atgaacggca catccgtgct
    19081 gccgacgggc actgccacga gcgaggtggt gggcgagtag tagaagaacg aaatccccgc
    19141 gccctcgcgc acaactttgc caccgctgtg ttgaatgaca tgcgtcgtgg gaggaatctt
    19201 gatgaatcga aggccgaaca taagcgtggg tagatgtgga gatttagcgg aatcaataag
    19261 gtggaagcaa gcggcaaaaa ccatgcgtga gcaccacgcc gagaaaaaag agatttctga
    19321 gcagcatggc aggccagagc acgcgtggcg gcgcgacgct ttcctgcgcc atgctgcgga
    19381 tgaaggtgac ggagcggggc cagtcgcgga ttcctgtgat ccacgccttt ggaatgccgc
    19441 tttcgcccgt ttcggtgccg cagatgcctc ccgcaatggc ggccacgctg tccgtgtcgc
    19501 caccgcagac gatgagttcg gtcaaaacgc gctcgaaatc gccgcgatgc cgcagccagc
    19561 caaaaagcgc cgccgcgacc gagttcggcg cgaaaccgct cacgccatgc tcgcagccga
    19621 tccgtgccgc gtagtcgctg acgctgattt tttcgctcag gccggtttcc atggctgcga
    19681 agcgcgattt catctcctca ctcgtcacca gcggtcgcag agtgtcgagg acggtttttg
    19741 tttccgcatg gtgtgccgcc agggccgccg cctcggccac cagcagcgcg gattcctccg
    19801 cgcgtgggtc ggtgtgggtc acccggcagg ctgcgagggc gaattcatgc cgcttcgccg
    19861 cgtcatcgcg aaagatcacg ccgacaatcg caccacgcat cgccgcgcca ttgcctgccg
    19921 atttcacgcc ggatctggag gctggaaaac cgtccatagc ctcaagatcg cccgcgccgt
    19981 gctcaatccc acgcctgcgg gcagcgccgc cagccaccag cgcaactggc ggccgaaatc
    20041 tcgctgcatc gcggccgcgt catcgcaatg cctgcgcagc gccgcagcca gcatcaacgc
    20101 gtgatctgtg tcgtcgctca gcattccaca accgaagatg agccggtgac gcaactcgcc
    20161 tcccgtccag cgttggatgt gccgccgagt caatccttcc aagggcaggc ccagcgtatc
    20221 gcccactgcc gtgccgagca ggcagccaag aacgggagat gcaggtgacg ggttcatagt
    20281 ggaattctcg ccgctcatct tttggcatcc atgagagccg tgtagcgatc atgatcctcg
    20341 cgcgaaaaac agcaaaaggc gaccgactgg accgacgacg gctccttcag aaactccgtc
    20401 accgcctcga acgcaatctt cgccgcctca tccgctggaa aacgatacac gccggtcgag
    20461 atgcacggga aggcgatgct ttgcagccgg tgctccgccg cgagcttgag cgagttcgtg
    20521 taacacgcgc gcagcaggtc gaactcgcga ttgccgccgc cgtgccacac ggggccgacg
    20581 gtgtgaatga catgccgggc cttgagtttg tagcccttgg tgatcttcgc ctcgccggtg
    20641 aggcagccgt tgagcttcat gcactcgcgg acgagttccg gccccgccgc gcgatgaatc
    20701 gccccgtcca caccaccgcc gcccatcagc gaggtgttgg ccgcgttcac gatggcatcg
    20761 acgtcgagct tggtgatgtc gccttggagg gcttggagtt cagcaggcat gggagttgtg
    20821 aatgggtgag ttttgaatca gtgagttctc aaacgaaggt ccaaaggttc gcgcgatgcc
    20881 agccagtccg ccgtaattcc gtcacgcccg acacgcaggg aaacgatgcc tccgacgatg
    20941 gcgcagttgg cgtcgcagtc gccatcacct tcgagagtgg cgagcagggc ctccgaatag
    21001 cagtcgagat gccggatggc gcaccagatg gcaaaaggca ccgtgtccgg tgcggtgacg
    21061 agaaatcccg agccgagaat ccgcgcggca ttctccgcag acatctccaa cggcacgagg
    21121 cgggctttgc gaatgccttc gagtgtttca ccggcaggtg tttgccccag aacggcgtcc
    21181 catatccatt cgcgcgcttt ctccagcggc agaatgcgtg atgcccatgc gccagccgca
    21241 gccaacgaga cggcgatgct gcccgcgaca ccttctggat gtgagtgagt cacgacggat
    21301 gacagagtgc tttgcgagac aatttgggcg ggatcatcgg caaaccatgc gcccaggggg
    21361 gcgacgcgca tggcgctgcc attgcccttc gacccacgac cgttgaacgc ggcggcggca
    21421 gtggttctcc agctctctcc cgcagcgatt tcctccaact gcattcgagc gcctttgccg
    21481 tatccgcgac cgggatcagc ctgataacgc tccgcaaagc gtcgggcgag tgaatccgtg
    21541 gcgatctggc cgtgcgcgac cagttcctcg acgatggcca tcgccatctg ggtgtcgtcc
    21601 gtgtgccacc aggggcctga gggcaatcca ccgggttcca ccacatttcg agcactgcgg
    21661 gcgcagctgg aaagcatctg tccaaacgcg tccccaaccg acaggccgtc gagggagagc
    21721 cgggtgcgtt cgaggcgttc tgcatgtgtg gaacttgtca tgggaatcgt tgggtgacct
    21781 cgatgaggcc gatgatgtgt tgatgaactc ttccagttct tccgcaggca cccagagttc
    21841 gcggtgcatg tcgccaccca cgacttgctc ggcgtagcgg ctgaggaaat cggcatcgac
    21901 ggcgaagcgg gtgacgaaac cggaaccgga ggcgggcaca ttccagtcgc gggcgatctg
    21961 gatggcgtat tcctcgttca tcacgggata gaagatcggc tggtcgggca gacgcggggg
    22021 gaaggctttc catccgcttt gttcgatcaa agcgagttct ttgggtccga cgggacggta
    22081 gagggtgatg gtgttcatgc ttcaagaagc tcacgcacct gcatcagcgc gaatcccagg
    22141 aggttgatgc cttgccaact ctgaggccgt tcggcatcag gatgatcggc ggacatgccg
    22201 atgccccaga tgcgatcgta agggctggct tcgacgatga ttcggtcgcc agtttgccgg
    22261 agaaactgtt ttagttccgg gtgctgggtg aacttggctg cgttggcgcg tacgacgatg
    22321 tcccagcgat gttggagcca gattgtcgtc gaagccggtt acctgccgac ctagcttctt
    22381 ggcaatcgcc ggacttttgg ccgcaagaat cgctgggaga tggacatcat cccggaaaag
    22441 acgcgccttt tcagccatca tgaaatgctc ggctgacggg tagcgaatgc cgtccacctc
    22501 gaaggcatgt ctgctccacc attggctgaa gcaggctttg gtaattgagc cgtcctttgc
    22561 ggatgtgtgg ccccaaaaga aggcccactt gggtcgaagt ccggcatgca tcgccagaac
    22621 caggtcatcg cgtgaaatgg gaagtttcgt tttcatatct caaacaagaa gcccttcttc
    22681 ttcagccgct catacttcac cggatcgaag cggaagagct gggcggggcg gtgggcgcct
    22741 tcctgatgtt tttctttgag ggggatgagg agatcgtggc tgagggcttt tttgcggaag
    22801 tttcgtttgt cgagttcctc gccaaggacg gcctcgtaga ggcgctgaag ctgggtgagg
    22861 gtgaatttcg gcggcagcag ctcgaagccg acgggctggt aggtgagctt gccgcgcaga
    22921 cgggtgagcg cggtggcgag gatgtcggcg tggtcgaaag cgagcggtgg aacgtcgttg
    22981 acgggaaacc acttggcctc ggcggcgtcg gtgtcggcgg tgacaacgga gggcttcacc
    23041 agcgcgaacc acgctacgct gacgacacgc tcacgcggat cgcgtttcac ggtgccaaag
    23101 gtgtaaagct gctccagaaa gacatcccgc agcccggttt cctcctccag ctcacgacgc
    23161 gcggcttcat cgagcgtttc ctcgacccgc acgaagccgc cgggcagcgc ccagcggtcc
    23221 ctgaacggct ccagggcgcg gcggatgagc aggacctgaa gctcgctgcc atcgaagccg
    23281 aaaacaacgc agtccacggt cagcgccgcg cgcggatact ggtaagtgtg aggcatacac
    23341 gatctctggt gtaaatttaa cactaatcaa tggtgaattt cgaattcaat ccaaggactt
    23401 ataggaagct gaatggacgg gactgttgag gggctttgct gccggtcatc gaaatatgaa
    23461 agccaatagc tccacgactt caacgccacc atcctccacc tcctcggcct cgatcacgaa
    23521 cgcctcacct tcgagcacaa cggcgtccag cgccgcctca cgaacgtgga aggccacgtg
    23581 atcaaagaga tgcttgcgtg acgaaatgtg cagcgtcacg caccggtcat tgtgtgatcc
    23641 agtccgggtt gaagcgtgcg aagtgaagtt cgtcccagta gtgggagctg cctttatccg
    23701 cgcgctcgta aacgacggcg atgcttccgt cagtcatgac tgcgatgtcc gaataagagc
    23761 ctggatgcgg ccagacggcg cgcccggccg accaactggc gccttcgtcg tgactgatcc
    23821 gaatggtgag gttgtagcgg ccgtaggggt gctccttttg acgaaatggc gacgccgggc
    23881 tgcaaaacag aagtgttttt tcatccaatc tctcgaggct gccatgacag cggggtgtga
    23941 tgagctcctc ctgacgccgg agcgcgctcc aggtttggcc gccatccttc gagatgctgg
    24001 tgaggtggcg caggttgtca ggcgcgtctt cagcgctttc gtttcgggat atcacgcgaa
    24061 tgccgccgtc attgagttcg atgaactggc actcgctggt gtgcatcccg atggtgccgc
    24121 cgagttgcca ggacgcgccg tggtcatcgc tgtaaaagca gtgggcaaaa ctgcgaaggc
    24181 ggcctccccc gatgtcctcg cggtggcgcg cgggcacaag cagccgtccg gatttgagtt
    24241 gcaccatcgc atggcccgga ccgcatccgt agcgctgatg ccaatctcgt tcccagcgct
    24301 gggcgtgact tttggggttg aagggttttg tgtccggctt gccccgcagt tggtcccatt
    24361 cgggtttggt gacctgatcg tgaatgacga cgggtccctg ccaggtgagt ccgttgtccg
    24421 tgctgctgag gtgggcgaac tgtttcttgt cctttaaaaa gaagagccag agtttttgcg
    24481 tgccgcggtc gaatccaagg gtgaggtcgg tgcagacgcg gtcctcatcg tcgaggatga
    24541 ggatttcccc ggtccaagtt ttgccgccgt cggaactgcg cttcatgacg atgtcgtggt
    24601 cgccgatgtc gccaggtccg tcattgcgct tttcacagac gacgaggagg tctccatttg
    24661 gagcctggat gagggctgga atgcggtagt tgggcttgtt ggctggagtc accggaaaaa
    24721 ggcaggtggt ctcgaaccag ggctccgcag cgctggcagg cagcgcgcag agtatcacgg
    24781 cttgaaataa aaaaattcgc ataaccggct tgagtaaacg ccgtcaagca tgcggcttgc
    24841 atggtttgtt gagctgacca caagcagcgc gctgtttccg gaaagctttc agccccgaat
    24901 ggagaataca ggtcaatttt accctccgaa gaatggataa aagcggttgt caaaaccgcg
    24961 ccggctgccg catggtgcgc gcatggctag agccgcacca gccaatcttg tcgtcgtagg
    25021 gtccatgaac gtggacttga tcgcctccgt gaagcgcctg cccgcgcccg gggagacggt
    25081 ttcggcgtcc gctttggaaa aacgcttcgg ggggaagggc gccaaccagg ctttggccgc
    25141 ggcacgacag ggggctgcgg tgacgctcat aggctgcctt ggggatgatc cggacggacg
    25201 tgattatcgc aaccacctgc gccgtgaggg ggtgaactgc agttccgtca acaccacgcg
    25261 cggcggcact ggcagcgcct tcatcgccgt ggacgcgaag ggggaaaacc agatcatcgt
    25321 catcccaggc gccaattccg ccctgacggc gccatctgtg aaggtgcagc gttcgcgcat
    25381 tgaggtggca aagacgctgc ttcttcagtt ggaggtgccc atggagagcg tgatcgaggc
    25441 catccggctg gcgaatggcc gccaggtgcc ggtggttttt aatccctcgc cctgccgcgc
    25501 ggactttccc tgggccaagg tgagggtgga ggttctcatt gtgaatgcag gagaggcgcg
    25561 gatgattttt gacgccgatc cggcggggat gcaggacggt gtcgcggctt ggcaggaaaa
    25621 actcaaggag aagaacattg gcatcctcat tgtcacacgc ggtgaaaaac ccacgcttgt
    25681 gctgcattcg gaaggcttca aaaaagtcgc cacgcatcct gtcaaaccgg tggataccgt
    25741 gggcgcggga gacaccttcg cgggcgcatt ctcagctttt ttcgccgaag gcatgccgct
    25801 gctggagtgc gtgcgctggg cgaatgccgc cgcggcgctc gccacgctca agcacggcgc
    25861 tcaggatggc attcccatgc gcggcgacgt gcagagcgcg ctgagccatc ccgaggggaa
    25921 aaaatgaggg ctttggcagc gcaaaaatgt tcgcgttttc ctccttgcgc cttcccggca
    25981 tctctcaatc ctccgcgtcc ccatgaccac tgtcactctc ggactcatcc aaggcggcac
    26041 tcatgcgggc aaggccgaaa gccacgcccg tcatgagcgg ctgatccgtg aggcggcggc
    26101 gcggggcgcg cggatcatct gcctgcagga gcttttcgac attccgtatt tctgcacccg
    26161 gcaggacacc gcgctctttg acctggccga ggcagtgcct gggccgacga cggaaaagat
    26221 gtccgcgctc gccaaagagc tcggcgtggt gctcatcgtc ccgcttttcg aaaagcgcgg
    26281 tcccggactc taccacaaca ctgccgccgt gctggacgcg gacggcgcct acctcggcaa
    26341 gtaccggaag atgcacatcc cgcaggatcc cggcttcgag gagaagtttt acttcacgca
    26401 gggcgacctc ggctaccgcg tgtgggacac gaagtttggc cgcatcgccg tgctgatttg
    26461 ctgggaccaa tggttcccag aagcggcgcg cctcgccgcg ctggccggcg cgcagattct
    26521 tttttacccc acagccatcg gctggctagc ctcggaaaaa gccgcgctag gcaaagccca
    26581 gcattgcgcg tgggagaccg tgcagcgcgg tcatgccgtg gcgaatggct gcttcgtcgc
    26641 cgcggtgaac cgcaccgggt ttcaggacga aaccgagttc tggggccaga gtttcgtggc
    26701 caacccctac ggcgagatcg tcgccaaagc ctcagtttca gagcaggagg cgctggtcgt
    26761 cccctgcgac ttccaggtgg tggaggactt ccgccgcatc tggccttttt tccgtgatcg
    26821 ccgcattgac acttacgcac ccctgaccaa acgctacctc gatgagtaaa ggatacccga
    26881 agaaccaccg cctgcccgct gaattcgaac cccaggaggc ggtctggctg tcatggccct
    26941 caaacaagga gagctgcccg aaaacatacc tcaagctgca ggacaagttc ggagagatcg
    27001 ccgcgaacat gacccgctac cagcgtgtgc gcatcaacgc gccggaaaac atgcacatgg
    27061 gcatccgtct cagcatcgcc gacaacgagg gcgcgctgaa catggtggac ctctacaacc
    27121 accagaccaa cgacgtctgg tgccgcgacc acggccccat tttcatcaaa aacgatacca
    27181 ccggccaggt ctccatcacc gactggaaat tcgacggctg gggcggaaag ttcgaggccg
    27241 ggctggacaa caccatcccc gagaaggccg ccgaagtgtt gaagatggac cgctatgtct
    27301 ccgacatggt gctcgaaggc ggcgccatcg agaccaatgg caagggcctg cttttcacca
    27361 ccgaggccgt cctgctcaat cccaaccgca acggcggcaa agcgcgcaag aaagaggagg
    27421 tggagaagga gctgaaggcc atgctcggcg tcaccgacat cgtctggttc aagaaaggca
    27481 tcgagggtga cgacaccgac ggccacattg acgatctcgt ccgttttgtg cgcgaggacg
    27541 ccgtcatctg catggtcgag agcaaggaga acgacccgaa ctacaaggcg ctcaagcaaa
    27601 tccgcgagca gctcgacgat gtgcgcaccg ccgacggctc caagctggaa attatcgaaa
    27661 tcgaaatgcc caacgccatt gaggccaggg actggcgtct cagccgtctg cccgccagct
    27721 atgcgaattt tctcattgtg aacaatgccg tctttgtccc catctttggg cagaagaaaa
    27781 aggacgccat ggccgaagac aagatcgccg agtgcttccc tgggcgcgag atcatctccg
    27841 tgacctgcaa agacctcgtc atggaaggcg gcgcgctgca ctgcattgcc atgcatcagc
    27901 ccaaataagg gtggcgcgtc cgtcctggtt ccagaacgca tcccagcatc caggtcaaac
    27961 tttcctcact ttccagttcc cgtacttccg catgaacgtc agcattttcg gactcggata
    28021 tgtgggtgct gtcaccgccg gctgcctggc cgagcttggg cacggcatcg tgggcgcgga
    28081 tgtccagcag gccaaggtgg acgccttcaa caccggccgg tcccccatca tcgaacccga
    28141 actcgatgat ctgctcggca ccgcccgcgc cggtggccgc ctgcgcgcca ccacaagcgc
    28201 cgaagaggcc gtggccgcca cggatgccag catcatctgc gtcggcaccc cgtcccttga
    28261 gtccgggcgg ctgaatctcg atttcgtgcg aaaagtcagc gagcagatcg ccgccgccct
    28321 gcgcgtctcc ggcaaacggc atgtcatcct tttccgcagc accatgctgc ccggaagcac
    28381 gcgccagatg gtgcgggact tcttcgaaga cttgcgcgtt tccggccagg tgtccgttta
    28441 ttactgcccc gagtttctgc gggagggcac cgccgtgagg gactttcgcg agccctccct
    28501 ggccgtcgtc ggcacccatg acgggcgcga gccggaaagt gacgaagccc gccggctgct
    28561 cggtggcacc cccgccgtgc tcgcctggga aggcgcggag atgatcaaat acgcctgcaa
    28621 ctactttcac gctttgaagg tgggcttcgc caacgagatc ggccgcctgt ccaaattcct
    28681 tggtgaggat ggcgcccgcg tcatggacgt ggtctgcgcc gacacccggc tgaacatctc
    28741 ccgctattac atgaagcccg gcaacccctt cggcggttcc tgcctgccca aggatgtcag
    28801 cgccctgctc tccttcgcgc gccaggaggg catcactctg cccctgctgg acaacacgct
    28861 cgacaccaac caggcccacc ttgacctgct catcaaactc atcaccagca aaggagcgcg
    28921 ccgtgtcggc ctgctcggac tcgccttcaa agccgacacc gacgacctgc gcggcagccc
    28981 catggtggct gtggccgaga cactgctcgg acgcgggttt catctcagca tctacgatcc
    29041 cagcctcaac ctaagccgcc tcatcggcgc caacgaggcc gaaatccagc gccgcatgcc
    29101 gcaccttgcc tccctgctta aagacgaccc gcgcgaggtg cttgaaagca gcgacctcat
    29161 cgtcgcctcg cagaaatgcg tccccctgga gcaactcgcc accctcgtta acgcgaacca
    29221 gtacgtcatt gatgtgaatg gctggcgcga gctggcggag ctgccctgga gctatgaggg
    29281 gctgtgctgg tagccggaag catcagcgtg ctttggcccg ttttttcggt ttcctggccg
    29341 cctgcggggc ggaattcagc gcgcaggtgt ggatttcata aggcgcccct gtggcactgt
    29401 cgaactcggc ggcggcgttg agtcctgccc gtacgatgtc caccacattg tcgaacgggt
    29461 catagaccat ttgcatggcg ccaagggcga aatcccgccc ggagccggcg gcccagaatt
    29521 tgtgaaactg ctgggcgctg cggtaggagt agagggcgaa gatgccgtgc gggttggcca
    29581 gcacgccgta aaactgcgtg gtctcgtatt cctcctcctt cgaggccatc gtggacatga
    29641 agtactcggt cttcatccag tggtgagcgt gacggaaggt ctcgaaaatc atgtccgtgg
    29701 aggagaagtc acgggggcgc tcgggattgg caaagtagct gttcatcacc acgaggctcg
    29761 cggatgtgcc ggtgaggccg atgaaagtgt ccccgatttt ggtgatcttg gttttgttga
    29821 cgacatggtg cgcgcgctgg cgcagcgagc ctaggcagct catggtgtca gcgccaatgc
    29881 aggcgactcc gtttttctgg gcgatgacga tggtggacat gcggcgcgta gtgtggggag
    29941 cgggtccgct ggcgccagcc ttttttggca tggacagcgc ggattttggc aatgcggatg
    30001 gaagttgcgc aaggcgtgca acattccccg ttggtgccgc atgacgtttc tccgcctgcc
    30061 catcctcttg accgcctcgc tgacgctgcc tttgaccgcg caggaggggc cgctgctttt
    30121 ccagacggtg tgcgcgcaat gccacgggcc ggagggcggt ggcagggacg agttgaaagc
    30181 gccctccatc gccgccctgc ccgcctggta tgtgacgaca caactggcga actttcacga
    30241 cggcaaacgc ggacgcgacg gcgcggcgga cccgcaaggc gcgatgatgg cggcggtggc
    30301 caaggcgctc tcggagacgc aaatccaggc tctggccgcg catgtggagt ccctcaagga
    30361 ggtggctccg gcggttctcg tggtcgaggg cgcggatgtg cgggcgggcc aggagctgtt
    30421 ttatgagcgc tgcatggagt gccaccgcta caacgcctcg ggtgagatgg tctttggcag
    30481 cccgccgctg acgggcaggc agggctggta tctgctggcc caactgcaaa aattcaaggg
    30541 tctgcaccgg ggcgccgcca agggcgatga aaaaggcgcg aagatggtgg agatggccac
    30601 caagttcatc gaggacgagc agacgatgaa aaacctcgtg ggctacattt tgacgctgaa
    30661 caagccgtga aatcccgccg ccggcgcgta tggatcaggc catgcgcatc atcacctgcg
    30721 gccccgagca cctggagtcc atccgcgcca tcttcaacga ggcgattgtg aactccacgg
    30781 cgatttacga ctaccacccg cgcagcgtgg agacgctgcg cgggtggttc gaggccaagc
    30841 aggcgcgcgg ccttcccgtg atcggcgtgg agaacgggga cggcgtgctc atgggcttcg
    30901 gcagctacgg ccccttccgc gcccttccgg cctacaagta caccgtggag cactcggtct
    30961 atgtggacgc gcgctttcgc ggcctcgggc tggggcgctt gctgctggag caggtcatcc
    31021 agtccgccca ggcgcgcggc ttccacatgc tggtgggtgt gattgacgcg gagaacgcgg
    31081 ccagcatcgc gttgcatgag cgcctcggtt tcaagagcgg cggcggactg cgccacgcgg
    31141 gcttcaagtt cggacgctgg ctggacctgg tgttttatca aaagctgctg cccactcccg
    31201 agtcgccggt ggacgggtga gatgaggctc cccgcaatgc ctccagcgct tccgtgatga
    31261 gccccggccc gcgatagacc agacccgagt agagctgcac gaggtccgcg ccggccgagt
    31321 gtttttccag cgcgtccgca ccgctgagaa tgccgccgac gccgatgacg ggagtgcctt
    31381 tccgcaggag cattcggaag gcctggacca cgagcgtgga gcgttcgcgc accggcgcgc
    31441 cgctcaggcc gccggcctcg cctgcgagtg gatgcccggc cacagcctcg cggccgatgg
    31501 tcgtgttggt ggcgatgacg ccgtccacgg ccagctcatt gaagacacgc gccagcgcct
    31561 cgatctgctg gccgtcgaga tcgggcgcga ttttcaccag cagcggcttt ttctctccat
    31621 gctcgccagc cagccgctcc tgctcggatt tcaaggcggc cagcagcgcg cgaatggagt
    31681 cctccgcctg gagatcccgc aagcccttgg tgttcggaga ggagatgttc acagcgatgt
    31741 agtttgccac gggatacgcc gccttcaggc aggccaggta gtctctcacc gcctcctcgt
    31801 tgggcgtgtc gaagtttttt ccgatgttca ctcccactac cgcccgggtt ttgcgccgcc
    31861 gcaggcgccg caccgcggct tcgatgccgg gattgttgaa acccatgcgg ttgatcagcg
    31921 cctcgtgttc cggcaggcgg aaaaggcgcg gcttcggatt gcccggctgc gggcgcggcg
    31981 tcagcgtgcc cacctccacg aagccgaatc ccagcgccgc ccaggcatcc accgccgagg
    32041 cggacttgtc catgcccgcc gccaggccga gccggttggg aaaagccagg cccatgcacg
    32101 agatgacgtc cgcttttctc cgggtctcat ccgcgcccag cgcgagcagt ccgcaggcat
    32161 gggagagacg catcatgcgt gttgtccatt catgcgcgcg ctcggcgtcc atgcggaaga
    32221 gccagggttt gagcaaaggg taaagctgcg cggcgagtga catgccactg caaagcgccg
    32281 cgcgcctccg ctgtcaaccc atggcgcgcg ccgcccgggt caccgctgca ccttggcgtc
    32341 gccgccggag gccagctttt ccacctcggg cagggtggcc atgctggtgt ctcctggtgt
    32401 ggtcatggcc agcgcgccgt gggccgctcc gtagtccaca gccttttgcg ggtcattttc
    32461 acgcaacagc ccggcgatga ggccggccac aaagctgtcc ccggcgccga tgcggtccag
    32521 gatgtcgaac cgtggatagt ccctgctgcg gtaaaaggag cctttgtgcc agcagagcgc
    32581 gccccagtcg ttgacagagg cggtgtgcac ccggcgcagt gtggcggcaa tggtttgcag
    32641 gttcgggaac tcggcggcga gcttctccat ggccgggcgc agcgtggcat cctcggcggc
    32701 aaagagatcc gcactgccgg ggatgagcgg cccggccgcg ggaggctcgt cgcagagcac
    32761 gccgatcatc acatccacaa aaggcgcgag acggcggttc agcgcctgcg cggcgggaaa
    32821 gccgccgcgc tcctgccaga ggctggggcg gtagttcaag tcgtagctga cggtgacgcc
    32881 gtgccgtttc gccgcctcgc aggcctcgat ggtcacaagg gcggtggact cggacaggcc
    32941 ggcgaaaatg ccgcccgtgt gcagccagcg gcagcccagc acgccgaaaa gatggtcgaa
    33001 atcgaagtcc cccggcttga tctgggaggc cgcgctgtgg ccgcgatcca cgctgcccct
    33061 ggcaccccgc gcgccgaagc cccgctcggt gaagttgagc ggattgcgca cgcgcctgcc
    33121 catgccgtcg tagggcgacc agcggatcag ggaagtgtcc actccgccct ggaggatgag
    33181 gtcttcgagc agccgcccga tctcattgtc ggcgaaggcg gtcaaaatgc ccgtgttcag
    33241 gccgaagcag cgccgcgccc cgcgcgccac attgtattcg ccgccgccct cccaggcggt
    33301 gaactgccgc gaggtgcgca cacgcccctc tccggggtcg agacgcagca ggacttcgcc
    33361 aagggagagg acatcatatc gcgggttggg cgtgggcatg gcagcgagca tggagaggaa
    33421 atcggaaatt cgaaattcga aattcgaatt tcagccgtcc cgcgcggggt tactccgcag
    33481 gctccatggt ggccttcatg gacccggtgc tctgcttcat gcgcgggtcg gcgccgtagt
    33541 cgtggatctg ctccacgcgc agctctgcca gctccttgtg gcaggtggcg acgatcacgc
    33601 gcccgctctc gtcaacttcg caggcgattt tgaatccgcg cgcctcatcg tagccgaaga
    33661 tttccaccat catctcgatg acgtactcgt atgtgtgcgt gtcgtcatcg tgcaggatca
    33721 catgccacgg tggctccagc tccggctgca tcttcggctg cgcgggcatc tggcgcgggc
    33781 gcgcgggcag caccggagta tcggtggcgt tggtcacggt ttgctgcggg cgcggaatca
    33841 cgccgccatg atgcacccgg cggcgctgcc gctcaagggg cgtgttcggc gcagtggcat
    33901 gacatttcgt ccttgatccg caaaaccggg gtcatgccgc cgcagttccg catggcgttt
    33961 gccgtgactc caacctgcca tccggcgtcc tggctcgctc cgcgcctact gtgtccgaac
    34021 tccggccatg acggagggtt tttcggtgaa ggaggtgtcg gtgagggttt tcaaaaaggc
    34081 gacgagggcg gctttttcct ccggggtgag gccgaggccg ccgtgcgtga ggtgtttggc
    34141 caggttggga tccagcgtgc tgctgctttt gatgccgcgg tcgtagtggt ccacgacttc
    34201 ctccagagtc ttgaagcggc cgtcgtgcat gtagggcgcg gtgagggcgg cgttgcgcag
    34261 ggagggggtc atgaatttgc ctttgtcccc gggtttgccg gtgactttct cccgcccgat
    34321 gtcgcggatg aggtcatcgg ggtcgagacc gttgttgtgg aactggttgt tggtgaaatg
    34381 ggcgcctccg tggcagtgga agcagtcggc gccgcgaatg ccgcgggcgg ggtcggactc
    34441 ggtgaagaag agccggaagc cgtgctgctc ctgctcggtc agctcggcct tgccggcgcg
    34501 tgcgagatcg agcctggact ggccggagat gaaggtgagg aggaattgct cgagggcgag
    34561 ggcgatgcgc tcgggggtga tctcgcgggt gccgaaggct ttttcaaagt cgccgagggt
    34621 gccggggtcg gcgcgcagct tggcaatgac gccgggcagg gtttcgttca tttcgagggg
    34681 gtcctggatg gggcggagga cctggtcgcg cagggtgggg gagcggccgt cccagaagaa
    34741 ggagggcttc catgcgaggt tgaaaagggg catggcgttg cgcgcgccgg tctggccctg
    34801 ggcgccggtg ctggtggcga gcggttcggc gaaggcggcc tgctggtggt ggcaggagga
    34861 gcaggactgg gtgttgttga tagaaaggcg cttgtcctcg aagaggcggc ggccgagggc
    34921 gacgccgtcg ggggtggggg tgttgtcctc gggccaggtg gtgaggggga agtgggcggg
    34981 gacgtggccg gcgagggtgg gaggggcgtt gacgctgccg acgagctcgg cggggccggt
    35041 gttttgcagg tcgggctgga tgccgaggag tgcgaaggcc tggacggcgt tgtcagccat
    35101 gcgcgcggcg aggccgccgt cgtcatggga gtgggtggag tcggagtcgg gaatgcggat
    35161 ggtgtgcgcg gcggcgaaga cgcgctcggt gtcgaaggcg agggtgaggc ggaggtcgcc
    35221 gttgaggtcc atctcgacgg ggacctcgat ggtgccggcg cagtcctggc cggcgaggtg
    35281 gtaggagtag ccgccggggt tttctccggg gcgctcgtag tggccttcga tggcgaggaa
    35341 gacgtagccg tctttccagc tccagtgcag ggcgttgagg tcggggttca ggggatggcc
    35401 ggcgggccag cgggcggggt cggacttgtc ggtctgctcg tcgaggccga gatcgaagcg
    35461 cagggcggtg tatctgccgc gcgggacgcg tggcagggtg aagtgggtgc ggtctttttc
    35521 gacgtcgagg aaggcgaacc agtcgccggc gctgatccac tcgccgcttt cggtgcggag
    35581 catggctttg gagaggaggt aggcgaggcg ggtgacgctg agcttgtctc cggcggcgtt
    35641 gaggaggccg ggctttgcaa gcggcagggg cgcgccgtgc cagcggtgct ggatctggac
    35701 ggtgaggcgg gcggcgggga gctggaggcg gattttttcg gcgatgcgcc cgagcaaggc
    35761 ggcgtgctgc tccagctcct gctggcggac ttcgggaatg ccggaccacc agccggattc
    35821 gacggcgtcg tgccggatgg cgcggtagcg ggcgaggcag cggtcgagct ggccccagcg
    35881 gcggatgatg gcgtcgtcgt cgcgcttggg ctggccgggg cggaggaggt cgttgaggag
    35941 gttgagggcg gctccggctc cggcgagggc ggcattgagg ccggacatgg gggcggtgcc
    36001 gtgggtgatg gcgcgctctg cgagggcgtc gagcttctgg cgtgtctctg gtttttcggc
    36061 caggcgggcg gcgagctggt agtggaggat ctcgtaaagc acggggtcgc cgggatcgag
    36121 ttcgtaggcg atgcgcagac gccaactggc ggaggcgttg agaaagcggc ggtgggcgtc
    36181 ggaggtgggg aaggggctgt tgcgcttggt gcgtcccgcc tcgagttcgg cgaggcggac
    36241 gatctgttta tcgagccatg agacgtcctc tccggggggg gcaggcttgg aacggcgcgc
    36301 gaagatgccg ggcggttctg gtggtggtgt tttggcctgt tccttcgtgg ttttgtcatg
    36361 gctgctctgc gcggcgcttt ggccgccgtg ccagtagctg tgcagggagt cgcggatgac
    36421 gcgcgcggcc aggctgccga aggcgctgcc ggcgaggccg aggggattgg tcatgtctgt
    36481 gacggcggct cgcgggccgg tcatgctctg acccgcccaa aaaagtcccg cagcacaggc
    36541 gagcgtcagg agcgtgtgcg gaaggcgcat agtccgagga gggaaagagc cgcgccctgg
    36601 agggtgagac cgccgagggt ggcgaggaag tccgccgagg ggagcggtcc cattttgaag
    36661 acaagacgcg gagttagatc cgccagatga taatgcggca cggcgagcca gagcgtctgg
    36721 atgaggctgg aggatgagcc gccgagcatg ggtgcgagcc aggcggagcc aaagaggccg
    36781 gtgaatagca gggccaccgg gacgaggaag gcgatgacct cggcggtgcg cgtgccaagc
    36841 gcggcggcga gcaggacgag cggggcggcg cagatgaagt aaagggctgt gtattgcagg
    36901 acgagctgcg tccagagcgc gccttcgccc gcgcgctgag gcaggcagaa gacgacacag
    36961 acaagcgcgg cgatgacggc cacggcgagc atccaaagca gcacggcgga gccgagctgg
    37021 agccagagat tccaggcgcg gcgcccgcct gagcgcaggt gctccagcat tccctcgacc
    37081 ctcaggcggc ggccaagcgc ggcggcctgg aagggcagcc aggtgaagag ggcgagccag
    37141 gcgaagaccc acgcggcctg ggcgcgcgcg ggctggagga tctggggatt ttcctgccag
    37201 ggtgtgacga gcggagcgag ccatgcgaag acgagcgcgc ccccgagcag cagccagcaa
    37261 ctgctgcggc tcatcagtcc ggccaggctc atgcggagga gggggaggga tttcatgact
    37321 ggagggagtg gtaagtgtcg agccaggtgg cgccgctctt ttcacgcgca tgggccagac
    37381 tgccgccgtg gatggagagc agggagtcgg cgtggcgcac ggactccaac tcgtggagga
    37441 tgacgaggcg gacggccttt gtctgcgcgg aggaccagag cgcggtcatc tgagcgcggg
    37501 tctcggcgtc gaggccggtg agaggctcgt ccatgagcag cacgcccgcg ttttcgtgcg
    37561 ccaccttcac ctcggcgagg atgagcagca ctttctggcg gttgccgcgg gagagctggc
    37621 cgatgggccg gttcaaatcc agacgcaggg tttcggccag ctcctcggcc atggcgagcg
    37681 cgccgttttt cagcagggag cggaagagtg tgcgcggttt gagctccgag tcggcgcgca
    37741 actcgtcgga gaagtagtgg acgtgcccgc cggcgttgac ggaaccgctc aaggctggga
    37801 gaaggccggc gagggtgcgc agcagcgtgg tttttccgga gccattcggg gccacgagca
    37861 gatgcagtcc gggaccggtg acgacatccc gcggagtctc gacgaggcag cgctgcctgc
    37921 ggtgccagag gggaccttca cgatagccga agcgggtgcc ggcggggatt gtcagagcgg
    37981 gttgttcaga catgggagta ctatcacggg tttctgtcat ttgccaacat cccataatcc
    38041 gtcatcgggg gcgccggagg gatcataaac gacgccgctg gtgtagagga gcgggggcat
    38101 gacattgtag tcgagtgtga gcgactcgcc cgataggctg acaaaggctg gcatggcata
    38161 ccagccttcc gggaggtaag tctgcaactg cgcggcgctg accagttcga tgtcgccggt
    38221 ggggtggtcg gccatccagg cgcgctgggc ggcaaagaca gcctggaggg agagggaggc
    38281 gtccttgccg cgctgccagt cacggatgga ggagacggtg agcccggcga aggtgctcag
    38341 acccaggagc agggccatga cgaggctgag ctcaaggagg ctgtagccgc ggcgggtgga
    38401 gtcagtcttt gtcgggcgag ggttcatggg cggtgagtgc ggcgggtttt tctgcgaggg
    38461 ctgcgagagc ggcggcttcg ctttgcggga tgatgcgcat ttttctgggc cgttcctgaa
    38521 tgacgggttt gatcgcagtg gcggcgggtt cagggcaccg aggtttttcc acaagacgca
    38581 actccggtgg cagggtggcg gcgcggtgga tgaagacgat gcgcgccgct tcaacggaag
    38641 gcacgccttg gctggcaccg gcggcttttg ccggcgcgcc gtccctgcgc acgacctggg
    38701 tgctggcggg gcgcggggca tggaccatgc cggggatggg acgcggcacg agtcgcggag
    38761 tcgggtcagc ccatgcggct gaggcggcga gtgcgaggat gaggatgtgc attttcatag
    38821 ggttcagggg tcacatgttg ccggactgca tcatgcgcgg gcccatgagg aagatgggca
    38881 gcatggagcc ggtgaagacg aagccgatga gcacgacggc catggcgagg gtgacgaaat
    38941 tgatgacttg ggtgaagtcg gacatgatgg cggcggtgtc ctcctcgtag atgtcccgca
    39001 ggagctcgag ctgctggggc agggaggctg accgctcgcc gatgccgatc atgtggatga
    39061 cctggggatc gagttcggtg tggcgcttga gtgattcggc aaagggcacg ccggaggact
    39121 cataggtggt ggaggctttg atgaaatcct cgtacatggg cgtgccggcg agcacgcggg
    39181 cggagagggc ggaggcgcgg gcgaggttga tgccgttggc atagagcatc tggagggtgc
    39241 cgatccacgc gctctggcgc aggcccatga cgagcttttt caaaagcgtc cagcggccca
    39301 tgaggaagat gagcagccgc tggcgcaggg gggcggagcg gaagatggag acgagaaagg
    39361 aggtgagtgc gatgacaaaa aagggccaga ctttttgcac gaaatggctg atggaaaaga
    39421 tgacctggga gagcgcgtcc ggctcctggc gcacgctttt gagggtgtcc tcgacttggg
    39481 cgacgacata gacctgcgag tagatgaaga ggccgatctg aaagaggatg atgatggagg
    39541 gcacaagcag ggcggagcgc agcttgctgg cgaacatcat ctcgatcttg atgcggcggg
    39601 ccagggagct gaaggcgtcg ccaagctgcc cggagtccga gccggcctcg atgatggtga
    39661 tgatggtgct gtcgaagcac tgctccttgg cgaaggcggc ctggacgggg atgccggcct
    39721 caagccggtt gcggatgttc atcaaggcgg cctgaagcgc ggggtcgggc aggccttgtg
    39781 cgtagtagag cagggcgtcg gaggtgctga tgttggcctt gagcatcgag gcaaggccgc
    39841 ggaaaagttt gatcagctcc ttgcgcggga tgccgccttt ggcgcggccg ctttttttca
    39901 cgcctttgag cggcttgacg agggctacgc ggaagccggg tttggcggcg agcgcggcag
    39961 cctgctcgcg gttgggggcc tcgatttcca ggactctgga gaggtccggg cgctcgctgt
    40021 ggcggagggt gacttggaag gcgttcatgg ctcacgagtc ataggtcatg tcgagcacct
    40081 tcttgagggc gtcgaggtcg gtcttgcctt cggccatgat tttgaggccg ttgtcgcgga
    40141 gggagggcag gctgctttcc ttgcgcactt ccagctccag ttcaaagggg gtgatctcgc
    40201 ctttggccag cttgtcaccg attttcgggc tgatgggcag gatttcgaga atggcggtgc
    40261 ggcccgagta gccgcggcct ttgcattcgg ggcagccttc tttgcggtgg acaaagacgg
    40321 ggcgcgtggc ccacgagggg tcgaggtgga agaggcgaat ctcgcgctcg gggacgccgt
    40381 cgcgccgctc gcggcaggcg gggcagagca gcttgacgag gcgctgggcg caggcggctt
    40441 tgagagtctg ggcgatcttc caccgctcga tgccgagctg ctggaagcgc tcgatgatct
    40501 gcgaggcgcg cggagtgtgg atggtggtga ggactttgtg gccggtgatg gcggcctcga
    40561 tggcgagttc ggcggactcg tagtcgcgca cctcgcccat gaggatgatg tcggggtccg
    40621 agcgcatgaa gctggagatc atgggcttga actcggagga gttgcgcagg tcgcaatggg
    40681 tgacgcctgg gatctcatcc tcgacggggt tttcgagggt gaggatgttg tcctcggggt
    40741 ggttgagctc gcgcagcatg gcgttgagcg tggtggactt gccggagcct gtcgggccgg
    40801 acatgacaat gatgccggag ggcacggcca tggccttgtc gagttcgaag agcgtctcct
    40861 tgtcgaaggc cagggtgccc atgccgaggc gggcctcgag atgcgtcttg tccagcaggc
    40921 gcatggtgac gtggtagccg cggtaggtgc ggtggcgctc gtagcgcacg ccgacggcgc
    40981 gtccgtagta gctggcggtg aagcgcccgg agatgccggg gcgcttgttg cgctcctcgg
    41041 gcgcgaggcg catgaggttg agcataaagg cgtccacgcg gtcgcgcagc ttcatgggca
    41101 tgtccacgcg cgcgccgagg tcgccgtcaa tgcggaagca atattgaaag agggtcttct
    41161 cggtcttgat gtggatgtcg gaggcgtgct gcttgatggc gtcggagacc atgctggaga
    41221 cgagctgggc gatgggttcc tcgtgctggc gggtgacatc gaagtcgggg cactcgttgg
    41281 agaattcctc gacttccacg gcctcgagtt cggcgcggct ggggccggag gagctggccg
    41341 agccttcgat gacggaggag acttcggagg caagagtgac gacttttaaa acctcgtcgt
    41401 ccggaaacct cgcggcgcag tagtcgtcgg cgcggtaatc ccaggggttg gccacagcga
    41461 gtagaatctg cttcgggctg cggcgcaggg gcacgaacat tcctcgggaa agcacggcgg
    41521 ggtcgcaata gccgacgaac tcctggtcca cgagatcacg cacctgcggg aaggcgggca
    41581 ggccggatgc gcggcccacg gcgccgagaa tgcgggtctt ggaggacttg cgcacaagag
    41641 cctcgtcgct cacgccttcc tgcgcggcca tggagctgcg cagctggccg acgatgagcc
    41701 gctggatggt gtcgttgaac tcgatgccga ggttgagatt catgcgaagt ggcgctgcat
    41761 gagctggctc caggaggcca ggtcggtgag gaaggaaaag acgaaaggcg agtctccagc
    41821 agtgacgcgg ttgagttcgt ggccgatcca aaaggaggct acggtggcgg ccacggggtt
    41881 gatggatgcc acatgcacga tgctggtctc gccggccatg atggcggggc agccgagggc
    41941 tccgcagatg gggcgcaggg cggggcagtg ctcgtaaagc gcgtccgccc ggccgggcag
    42001 ggggccgaaa ctccagtagt ggacgcggtc gctgaggctg gcgcggagga ttttctgcat
    42061 gcgcagcacc tcgccctcga cagtgaggga gtgcttttcc gcctgggtct cggcgatgaa
    42121 gatgagagcg tcctgagggg gtgacgggcg gcagatgctt tcgcggtagg aggaagtgag
    42181 caaggcggcc tcggcttctg aaaggtagcc gccgaggcgc agatgcttga cacatgtgac
    42241 ggcgatgcgc tccagcaccg gccggatgtc ggagagtccg ggaacggtgg cggagagggg
    42301 aatgccattg actgcttcgg cgaccatgcg ggttcagcgg tagggggctc cgccatgagt
    42361 gccggacgga cctgccgccg gagactgcac catggcgggt gatgactttt tggtgaagac
    42421 gcgccggagc cacgactccc tgggcgcgga cgccggaggg gcggccctgg ccgcaaggcc
    42481 ctggttcccg ccgccatctc cggtgaggga aagcccggaa tccgcgatgt cctcgggctg
    42541 catggctgaa tactcgcgca tctgccagtt cacgccgggc agttcggccg cgtttgaggc
    42601 atcataaatc tggggggtga tgatgaagac aaggctgacc tgctccagct tcctctgctc
    42661 ggtgctgaac agtttgcgga tgacggggat gctgccaagc acgggcacct tgttctcgcc
    42721 ctccgagccg ctgtagtcat agaagccgcc gaggaagagc gactgtccgg ccgggatgcg
    42781 gctgatggcc tctacagtgg actcgctgac gcgcggatac acattgccgc cctggccggc
    42841 gacgagctcg acgatcttgg ccacgcgcgg gcgcagcttc atgcgcacgg tgccgtccgg
    42901 caggagggtg ggcgtgacgg agagggtgac gccgatctcg cggctctttt cggggtttgt
    42961 ggtggggtcc ggatcctttt cgtcaatctg gtagcggacg agttcgctga tgttctgcac
    43021 tcccgcgccg ctgtttgtca cgttggcggt gatgaccggg aagcggtcca cgatggagat
    43081 ggtgccctgc tcgttgtcct cggtgatgat ggttgggcat gcttcctggc tgacgatgtc
    43141 ggcttcctcc agggcgcgca ggatggcctg cacctgggga ttgccaaaga cgaggccgat
    43201 gccgtctgtg ttgaccttgt tgatgctggt cgtgggattg ggcagcgcgc cggagcgtgt
    43261 gatggtgctg atgtcaggca ggttgaagag ggcattcaga ctctgcgagg cggtgatggt
    43321 ggtgcccgtg tcgccaagcg cccgactcca gtctatgccg gcgcggcggg cgtcgttgcg
    43381 cttgatgcgc agcacgcgga cgttgacgag gatctgctgc ttggccttgt cgagctgggc
    43441 gagcagggcg cggatgcgtt cgagcctcac ctggttgtcg gtgacaagaa gcgtgttggt
    43501 tttttcctcg aactcgatct ggcccacctc gcgtgtgagc agcgggcgga tgatggcttt
    43561 gagtttctca aagtcagcct gctgtgatcc gccgcctcca ccacccgtgc cgccgccccc
    43621 atccatgcca tccacagtgg ctccaccgcc gcctcctcct cctccgccgc cctggttggc
    43681 gcggttgagc ggcgctccgc gaaggtattt gagctcataa ctcatgacct caagaggcag
    43741 cctggagagc tgcgcatcat tgagcaggct gacggtggag cgctgctggt aaacggtgag
    43801 accgtaggcc tgggccaggt cctccatctg ctggcgcgga ttgtcgagct tgagatgacc
    43861 ggtgacattg aaggcagggg tggccagctc gttgttgtga aagaactgca gtccggcgct
    43921 gcgggcgagg tattggaaga cctcgttgat gggggcgttg tctatccagt atccatcgtc
    43981 gggcagggcg ggcaggctgc tgacggtgga ggccgccgcg ccgagcctgg aggagacatc
    44041 caccgccgcg ggggaggccg cgtccagggt gccagtggcg gcggggtcct gcgccgcgcc
    44101 catgagatgc aggctggcaa ggctggagct gatccatcgt gtgaaaaggg cgctcatggt
    44161 gacttggggt tggccggctc catgggcgag actttgccgt caagacggga ggcgatcttg
    44221 tgcatgggct ccagcggcag cgcggggatc atggagtgga ggagaacgcc ggtttcatcg
    44281 cggaggaggt tgcggaagcg cagctcggtg tggccaacct cgatgacctg ggcctggaaa
    44341 gtctccccct tgaaggtcat ttcaatgacg tcgccctcaa agacattgcg cccgccgatg
    44401 aggaactctt tccgcgcgag attgacaccg ttgatcttga gggactgaag ggcctggttc
    44461 agcgtcacgc gcggggcctc gcgaagggtg tcgtcaaaga agaccggcgc ggcgtcttcg
    44521 cgcgggaaga cggcgaggcc gaaggggtcc aggttgcgct ggcgcgcgcc gaggtttttg
    44581 gcggcggcct caatgtccag cgcggtgggc aggatttcca cggagggcag ccatgaggcg
    44641 gtgcctttgc cgatgaacaa ggcggagcgt ttcggcctgg cgtccgcggg tttctgcgcg
    44701 ccggcgccaa gggcgaacga gaacagggtc atgattgtga gcgcggttct catggcttgg
    44761 cctccccggc tttccagcat gtgtgcgtca tttccatgac aaggtgctcc tgctgggtga
    44821 gcaggtgcgc tttgcgcgtg attttcatgt cctcgagcac ggcctggggc atgagggatt
    44881 ccacctggag cagggcctcc tgcatggggc cgaagcctcc ggagaagctg cttttgacgc
    44941 gcgcggcgtc ggttctcaaa gagcgcgcca cgcgcccgcg gtcgagcgga aagacgaggc
    45001 tgccgcgcgc cagttcgatc tccttgaact tgggcagcac ggcgtccagg acacgggtga
    45061 aaagctggcc cggatcggtt tcgagcagct ttttctggtc ggcgaaagtc ttgcgtttcg
    45121 gggcgatgtc ggcctcgagc tttttcatgg cggccagccg ctgggtctgc tcttcaaagc
    45181 gctcctcctt ctccttgcgg atgtccgcca gcttgccgag cgccgcgagc actccgccga
    45241 gaaggagcac gttgaacaca gccggggtga cgaggccgaa tcggatgatg ggctgggagt
    45301 aggcgctcat gaccgggatc aaaagggctt ttccaaaggc agtgccaggg tgtctttttc
    45361 agagatggtc tgcgagatct ggatttcgaa attgaagggg aaagccagcg tgggatcgcg
    45421 cgccgcgtcc gccgccgtgg cctgcgagtc atagcgggaa ttgcgcccct gggtgagtga
    45481 gacggtgatt tggcgggccg ggtctggctt gaaaccaggg tcgtccgtgg ctccggccac
    45541 ctgctcgaag aaggctccga ggccgcgctg gctgttgagg gagttgagca gctcgagggc
    45601 cttgggtttg acgaggcctt tgatggtcca gttccgcacc agcccggtgg cgtcgttttt
    45661 ggagcctttg cctttggaac ctttcgagga gcgcgaggat gaagaggcgg cggagcgggc
    45721 tgcttccagg gagtagctga agttttccag cctgaccccc gagtcctcgg ggaagagctg
    45781 caggagaaat tccagcgtgg tccatccgcg cgagcgcggc agcagcagct gctcggtgag
    45841 ctggatctgg gtttgctcgt caatgagctt ggcgtgcttc gccttggtca tgctgacctg
    45901 cgactccgtc agctcccatg agggatggtt catggcggag aacaacgacc aggttccata
    45961 agccccggtg ctgacgagtg tgaagacgag cagaacaaca aaccaggtgg agaagcgcag
    46021 catgcgcagg tcgctcagcg tggggtagag cgtgtcgaga cgcgccaggt ccatgaaatt
    46081 ctgcccggcc cagccctgcc agagcgcggg gaacgtttca gtgccgccca gaggcgctcc
    46141 accagcgcgc acctgctcaa tggcggccga gtcgtaaatc atcagttcca tcaagttccc
    46201 tggaatggcg gcgagctgcg gcaggcttcc caggtccaaa atttcgcact ccagttcctg
    46261 ccggctgagc gagtaaagct cgagatcctt cgcggccgcc tccacggcgc gctggttgga
    46321 ggaggcgagc aggaggcgcg gtttgccggc gtggtccgcg ccggcgagtt cggcagccac
    46381 agccatgttc cacaaaatgt cgccaaaccc tgccggtgtg agtgacccgc ccctgtgtga
    46441 gaggctgcgc gccgcgcgca gctcgccctc cgggctgagc gcaaaaacgg cggtgtactt
    46501 caaatagggc atgacgacca gcctgccgcc gcgcgcttca ggctggatca agggcagggc
    46561 ggccagcgcc tccaaaggcg cgctggtcac ggagacacgg actggcaggc gcattttttc
    46621 gccctcggcc atgagcttct gcataaaggg ttcgcgcgcg cgcgaaagag ccatcgccac
    46681 cgagcgagtc tggccgggac ccgcgcccca gaatggcagg agacgccagg aggtggtgtc
    46741 ggtggagact tcacgatccg ccagcatctc cagcggctcg tccaccaaat tgtagcggag
    46801 aattttgaaa tcctcccccc cctcggcggc ggcttcgagg gtctgcgcca tttccgcgag
    46861 ggcaaactca tccgccacat gcagaatgac tcccagggat ttgggtttct ggctgtaata
    46921 ggaggtggaa atcaactcgc cgagcagctc ggatgcgttt tccgccgtgg tcagactggc
    46981 cagcggcagg gctggtccgg gccgccactg gttgtcgagg cgggagaagg ccgccgaggc
    47041 gacctctctc ccgccgtcga accagatgtg ccattgccag gccagatcgt ccggctcccg
    47101 gtaccagtcc ccgtccgtgc ccctgctcgt cttggatgcc accgcgcgcg cctgacggta
    47161 aatgtcagcc gccttgagtt cgcagggatc ggtgatgctg agcatggaat tcgggaggga
    47221 atggttgccg ggacgccggg atccgatctg tgttttacca atctgttgtg tcagcttcca
    47281 ccagcacatg ggtggacgtg gcacccgtgc cttcatagtc gcagttgccg tagggcgtgc
    47341 cgactgcggg gacggtggct tcccaggcgt attcaccacc ggaagggcag gcgggctcgt
    47401 tttccataag caggccggca ccgaagacat cggtgctctc cagatcggcc ccgatgtcga
    47461 ggttctggaa gttggctccg gcgcggacgg ctttttggac agcggcgagc tgcattttgc
    47521 atttcgcctt gtcagcgccg gcacggtagg tgccaaggcc gaggaagacg acagcgatca
    47581 aacccagaag gagggcgatc accagactga tttcgatgag agtcatcccg gaggcgttga
    47641 ggcgcttggc gagacgagtg ttgtttgtgc gattgatgtg tgttttcata gtgaatgtgg
    47701 aatttataat ccacaccgca attctctcct ttgattatca attgcgcaaa caaaaattct
    47761 cgttttttta ataatgcgac taaattatag caaatagata agccattggc ttggtattgg
    47821 taagaaatca gatggaaaat cgcttcacat ctgagatgtt ccgctcttgc catgctgcca
    47881 atgtcgctgg aattggcgaa ttgagtgtca cccctgatta tcgaattctt tcgaaataaa
    47941 tgtccgtgaa acaatataaa accactcata aagagagctt tatgcaatac caccgtctgc
    48001 gccgctcaag cctgagccgc cagagcggca gcggcttttg ctttggcctc ttcggctttg
    48061 cggaggattt catccgggct gggcagcgag gcgagtacat ccatgggaat gaacccatca
    48121 ctcacgagct tggtgaggca ttttttgcgc tcgtcgagcg ctttgtcagg actgcgctcc
    48181 ttgctgaagc ggctcttggc ggcggcgctg cggtctttga gcgccgcgta gatgtcgccg
    48241 cctaacaaag aggaaatatc cactttcatt ttctcccggc cgtggtcggc ggcgctgacc
    48301 tcgtcgccgt tgatggggcc gccctggtat ttcgggaaca tgatggcgac ttcggggcat
    48361 acacgggagc aggcggggca gttggtcttg cagttgtcgt tgttttggac ctggattttg
    48421 tggtcggcgc tgacgccata gacatcgaag aggcagaagc tgaggcactg catgcagttt
    48481 gtgcagcggg tgtagtcaat gacggggaac cagggcttcc atttgccggg ctcgttcatc
    48541 gggcgcgcgc cgcgggtttg ctcgacaagc gagaccacgc cctccgcctc cagaccggtg
    48601 atgtcccgcg cctcgatttt gattctgcca gccgcgtcct cgatggcggg ggcctggcgg
    48661 tgctcgaagg cgccgagcac cagaagcgtg tggtcgtccg cagggcgggg ggctgcggac
    48721 gcgccgggag tgacgcgcga gacggagtag cctttgtcca gcagggccat catgactttc
    48781 gcccgcgcct cctgaggcag cggaatggct ccgttcccct catagagaac gactttcaaa
    48841 gggcgggcgg tgtgggtgat catgcggcaa aaaatgaggc tgtgaagccg ccggtcaaca
    48901 taaacggggc cgcaagtcgg gatcacgccg ccacttttgc cgccgacagg catttgaacg
    48961 cgccgcctgc cccgtccggg tggagaaacg atggcgggag ggcggctaga gaggcgggtt
    49021 ttaagtgggc gcgaaaaaaa tccgcgagtg tcatgtactc gtgttcctgc cagccgagcg
    49081 aggtggtcca ggagcgcagg tcggtgaaat tgatgtcagc ggtgatgtcc tgccgtccgg
    49141 gatttgcata aacctccgct ccctccagcc tctggtggag caggtaggcg cgcagggtgc
    49201 cgcgcgggcg gcggtggtag agggcgggaa aaagatcgcc gtagtcaatc gcgagcatgg
    49261 cccccgcctg ccaggcgggg gcccaggagc ggaaccagtc gcgcacgggg cggtgcagct
    49321 cgcagcgctg gccgggcggc gggttttgcc actcgcgcag cgcgctgaac tgcgagcgct
    49381 cctgctccgg ccagtccagc ggacacgggg tctcggctcc ttggtcgagg tgaatctcac
    49441 tccactcgcc gtcctgccat tcgatgagat ggacgggaaa ggcatccagc agttcgttgt
    49501 ggtagatgaa agctctgccg cgcgccgcgg agagggcgtc ttcaaggcgt gaatgccact
    49561 tcacgccact gcccagacgg cgccgctgcg ccctggccag cacgggcgag ttttccacca
    49621 catgccagcg cagggagcgg cggcgccacc agcctgaaac tttgcgaacc gaggccatca
    49681 gggagccgtc cccggctccg acttcgatga cgtggcggac atcgggcatg aagcgcgttt
    49741 gcatttggat ccaggcggcc acggcctgac ccagggcagg ggagagcgtg gcggcggtgg
    49801 agaagtctcc gcgcgggccc acggtgtgga tgcggcgcgc gtagtaacct gtggcgggat
    49861 catgcagcgc gcgcgccatg aaggctgcaa aggactccac ttcagcaccc tgaccgtgcc
    49921 tgctcactgg ttggcctgga gctcctcgat cgcccatttg tcccaggcgg cccagtcgtt
    49981 tccaaaatcc tcgccggcga ggatggtgag cacgtcgcgc acttcctggc tctggggatg
    50041 ctggggattt ttgaggatgt tcatgaacag gggcatttgc agttcccggt tgcggttcat
    50101 gaggtcggag aaaagcacgt cgaggacttc ggggttggtc ttctggtcca cgagaagtcc
    50161 ggcgatgagg ccgtagtcct cgtcgctggt gagattgacg agatggtggc tggcgttgac
    50221 ctgggcctcg ccattgagct ttttgacgag ttcatgcagg tcgcgcgcca tctgcgggat
    50281 ttccttgtcg gagcggagga tttcatcaat gagaacatcc ccctcctcgg cggtcatgag
    50341 ctgctggccg ggttcggttt tcttctcagc aggcggcgtg gtaggctgag gagtgctgag
    50401 caggttgggc tcggtcttgg tgccggcttt gtccttcgtt ttggtgacgg gcgcgggagc
    50461 ctcaggctcc ttgccggccc attgccagcc gaaccagagt gcgatgacga gtccggcggc
    50521 tccggcgatg aggagcgttt tggttttggc gggggatggc gtgctcatga cggtgtgaag
    50581 atgctggatt cgcgatcctg ctcaaacaag tggcaagatt ttctcaattg cggatttcct
    50641 cgcccgtgga ggagctggag attttgactt cctcgcggtg cagagtggga tcgctgcgga
    50701 aggtgaggcg ggccagatat ttgcgttcaa ggtccacgag cagctcgccg tcctcggttt
    50761 tgagacgctg catcacgtcg gggtggacga cgacgatgag gttgcggttt tcctccctgc
    50821 ctgtgccgag gatggcgctg aggcggcgct ggagttcgac gctcatcgtg gtggcggtct
    50881 tgacctggcc gtggcccttg cagtaggggc agacctcgta aagcgcgctg gagagcgact
    50941 catggaggcg ctggcgggtc atttccatga ggccgaactg gctgatgggc agcacctggg
    51001 tcttggcctt gtcccgcttg agatgctcca gcatggcctt gtaaacggcc tgctggtcct
    51061 tgcggtgctt catgtcaatg aagtccacga cgatgaggcc gcccatgttg cgcaggcgga
    51121 gctggcgggc gacctcctgg gcggcttcga ggttggtttc gaggatgagc ttgtccacat
    51181 ccttgccgcc tttgttgcgt ccggtgttca cgtctatgga gacaagagcc tcggtttgat
    51241 caatgacgat gtaggcgccg cagggcagcc agacctggcg gtagaaggcg ttgtctatct
    51301 gcttctggat gccgtagtgg tcaaagatgg cggtctggcc ttgataatgg gtgatgcggt
    51361 tcttggcgcg gcgtgagatg tgcgcggcca tggcctgcat gcgctcgacc gtgggcaggt
    51421 cgtcgcaggc cacctcgtct atctcctcgg tgaggaagtc gcgcacggtg cgctcgatga
    51481 gctcgggctc ctggaagcag cagacgggtg ctttgtgccc gtcgcgcacg gcgacgatct
    51541 cgttccactc ctcgatgagc aggctgaggt ctcggatgat gtggcggtcg cgcttgccgg
    51601 atgcctccgt gcgcatgatg aggcccatgc cttcgggcac gttgaccttc tcgacaattt
    51661 tgcgcaggcg cgcgcgctcc ttggggtcct cgaccttgcg cgagatgccg aactggtcgt
    51721 tctgcggcat gagcacgagc aggcggccgg cgagggagat gttggtggtg actcgcgggc
    51781 ctttgttgcc gatggggccc ttggtcacct gcaccatgat ctcagagccg acgggataga
    51841 ggctggggat gtccttggcc tcgatgcgct tgcgtttttt tccgccgccg ccccggctga
    51901 cctcctccat gccctggttg gaaagggcct cggggatggc gtcccaaaag tggaggaagg
    51961 cgtttttgtc gaggccgatg tcaatgaaca tggccttgag gccctgttcg atgtttttga
    52021 cgcggccttt gtagatggag ccgacgatgg tgctcgtgcc ggttcgttcg atggtgtact
    52081 cctcgaggat tccattgtcg agcagggcga cgcggttctc cagcttctcg cagttgatga
    52141 cgaggcgggt tccttgcctg atgtcttttt ttccagtgat gagttctttg atccgttgga
    52201 cgattcctag aggcatgatt tgaaaggttg gggttgggtg aaaatgttac tggcggaaaa
    52261 agcggcgcag cagtccgccg ccctgggcgg gctgttccga aggttgcgaa ggctggtccg
    52321 ccgaacgacg gccaaaaatg cccgcccgcc gcactggcac ggccttgggc acttcggcgg
    52381 cgtttttcac tgcggcgcgg ccggcggtgt cggcttcgcg cttgatgctg ggcgcggggg
    52441 tctggcgccc gcgcggggag gtgtcggtgg actcgcgctc ggattcggtg cccgtgtcgc
    52501 cgtcctcctg ggcggcgagc gcggcgagca ggttcggagt gtcgccgtag gaaaccatgt
    52561 caatcttgtt gaagttcccc ttcacttcct cgaccggctc gggcttgatc tcatatccat
    52621 tgtcgagggc gagggattgt tcgagcacgc gctgcgccac cggggccgcg caaccgccgc
    52681 cggacttgcc gccctggacg aggatgcaga cggcgtattt ggcgttcaca tagggggcga
    52741 agctgatgaa aagggtgtgg ttgtcctcga cacggacatt gcgctcgtcg cgcctccagt
    52801 tttgggcggt gccggtcttg ccggccacct cgacgccttc gatgcgtgcg gcgcgggcgg
    52861 tgccgccctg ggcgttgacg accttccaca tgccgttgcg gacggtttcg aggtctttgg
    52921 ctgggaagtg ctgggcaagg tcggcgcgca tttcgggctg cttttcgaag acgggatgat
    52981 ctccctccat gacttttttc agcaaatgcg gcttccagga cttgccggag gcgacggcgg
    53041 cggtgacgca ggccatctgc agcggggatg cgagcacgct gccctggccg atggagacgt
    53101 tggcggtgtg gccgttgctc cagcgctcgc gcgggctggc ctggcgcagc cagtcgggat
    53161 tggggaggat gccgccgtcg tcctcctcaa gttcaatgcc ggtgcgctcg ccgaggccga
    53221 gcattttgcc gacgcgtgtg atgttgtcta tgcccgcagt gttgccgtat tggtagaaga
    53281 agcagttgca ggagcgcatg agcgcgtcac tcaagccgag ggagccgtga gagcctccgc
    53341 tctggcgctg gatccagcac tgcatggcct tggtgccgta ggtgacactg ccggagcagt
    53401 tgaagtggtg cccctggatt ttggccaggc agcccgccag ggcgatcggc accttgtagg
    53461 tggagccggg gacgaagctg cggatggagc ggttgagcag gggcacggtg tggtttccca
    53521 gcagttcgtt ccagtcagcc tgcttgatgc tggggatgaa acggttgggg tcatagttcg
    53581 ggacggaggc catggccagg acctcgccgt tttgcggtga gacgacgaca acggagccgc
    53641 ggccgatttt gccgtctatc agcgcgcgct ctgcgatata ctggatgcgc gcgtccaggg
    53701 tcagatagac gtcgtcccct ttgcggggct cctcatagct gacctcgccg acagggcgcc
    53761 cgcgctcgtt gagctgcatg acacgcacac cgggcttgcc gcggaggtgc gagtcgaaac
    53821 ttttttccac gccggcgccg ccgtactcgt ccggaacata atagtcccag cccttgcgct
    53881 cctcggggga gacgcgctgg tcgtccggca tgcgcacata gcccatgagg tggcaggcca
    53941 gcgagccgag gggatatacc cgcgatccgc gcgccgccac cgtgacgccc ggcagggcca
    54001 ggttgtgctc ggcgaaatgg ctgagctcgg cgaaggtgag gttgtcccgg taaacccacg
    54061 ggatggcgcc tccgaagctg cggtaatgca cgcgcaagtc ctgctcggcg ttgaattcgg
    54121 cggcgagccc catctcgttg agccggctga cgatcagttc ctggaacatg gtgacgatgt
    54181 ccgcctcctt gcgtttgcgc ggcagtccgc ggtcgaggaa ttcgaagtcc accgtcggaa
    54241 tgtccatttt cttgattttg cactggcgga cgtactcgtc atacacatcg cgcaggttca
    54301 cctgcacttc gaaaccggcc ttgttggtgg ccagcacgat gccgttgcgg tccttgatct
    54361 cgccgcgcac tccggggatg cgcgcgcgct gctggcgcgc ctccggcact ttgttcacga
    54421 attcctcatg gcggtctatt tgcagcgacc acagcctgta cacaagcagc ccgaacccgc
    54481 acagcatggc gagtgtgagc aggtggagac ggaggcggta tttaacgacc atcgaaggag
    54541 aaggtgaggc cctcgtactt gatctcgtaa cgggaaaggc cggccaggga gtaaagcagc
    54601 aggaagatga gcggcgaggc gagcatcgcc agcaggctgg cggtcaccat tttcacccac
    54661 acaccgggcg ggaaaaagag cgagccgcgc aggaaggtca tcaccaggta ttcggtgagc
    54721 agccaggcaa aggtggtgaa gcccaccagc agcacgggaa gctccagccg gccgcgcttg
    54781 aagagcgggc gcacgccttg aaggaagctg ccgagcaggc cgaacaacag gatggagatg
    54841 ccgaatccaa aggctcctgg agccgccgcc tcggatgacg ctcctgtcat catcgccccg
    54901 gaggtggtgc tggccaccga ggctgccacc ggcatgtggc gcgcgtccca cagcccgccc
    54961 gtcacaaagg cgagcacgag catcatgggg aaggggacgg ccacggacgc cgcgaagaaa
    55021 aacaccggcg gcagaaacag cgtggcaaac tgggccatgg gaatgccggg tatgaactcc
    55081 tgcaccacga aggtgagcag cagcaggagg aaggtgaccg gataaaaaac catggccgga
    55141 ttacggttcg gtgtctgact gcatttccac cacgaacaca tgctccaggg tggcgaagtc
    55201 cgccgcgggc tcgacgacgg ccactccgta gagatccttg atttcaaaac gcttcacccg
    55261 cccgatgagc aggccggggg ggaagacgcc gccttcgccg gtggaaatga cattggcgcc
    55321 gggattgatt tgcgcgcctt tgccgaggaa ctggaggcgc aaatccgggc gcatgtccag
    55381 cccgccccgc tccccggcca ggatgccctg ctccagcgtg ccctccacct tcgcggacac
    55441 gcggcacatc tcgtcagtga gcaggatgac ttcggccatg cgcggagcca gcttgcctgt
    55501 tttgccgaca agccccgacg cgctgatgac tgggctgtcc gtggcaaggc cgtcgaggga
    55561 acccttgtca atgatcatgc tgttccacca ggtgctggac acgcgcttga ccacccgcgc
    55621 ccccgcgagc ttgaaggggc tggcctgctt gaactcgatg agctcgcgca gcttgttgtt
    55681 ctcctcctgg gtctgcgcga actgctggga gacgatgcgc agcctttcga cctcgatctg
    55741 caggcgctcg ttttcgatct tgagctggcg cgggtcaagc gcgggagcgg cggcctcggc
    55801 gacgccgctt tcgatggccg cgctggagtg gatgaagggg gacagcgcgg ccatcacgcg
    55861 gccctggatg tcccgcgtca cgggcgtgtc cagtgtgaac acagagacga ctcccgcgag
    55921 gaagatgccc agcgccagaa tgttcagttt gttcatgaga atgccttgtt atgtccgcca
    55981 tgggcggccg gcgttttggt tggccgtttt cccgcccccc ggctcagact tcggtgctgc
    56041 tgaccttgcg catcagctcc agctcgctga gcacgcgtcc ggtgccctcg cccacggcgc
    56101 tgagggggtc ctcggccaca tgcacgggca gggccgtctc ctcctgcagc agcttgtcca
    56161 gcccgcgcag cagcgcgccg ccgccggcca gcatgatgcc gcggtccacc aagtcggcgg
    56221 agagctcggg cgggcagcgc tccagcgtgg tgcgcacggc gtcaatgatg gcgttgagag
    56281 gttcgagcat cgcctcgcgc acctcctggc tggtgatggt gatggttttc ggcaggccgg
    56341 ccaccatgtc gcgtcccttg acctccatgg tggtctcctt gcccagcggg aaggcgctgc
    56401 cgatgcgcag cttgatctcc tccgccgtgc gctcgccgat catgagattg taggcgcgct
    56461 tcatgtagtt gatgatggcc tcgtccagtt cgtcaccggc cacgcgcacg ctgcggctgt
    56521 aaacgatgcc gctgagcgag atgatggcca cttcggtggt gccgccgccg atgtccacga
    56581 tcatgttgcc cgcggcctcc tgcaccggca ggccgacgcc gatggcggca gccatcggct
    56641 cctcgatgag gtaaacctcg cgcgcgcccg cctgctccgc gctttccttc accgcgcgct
    56701 tctccacttc ggtgatgccg gagggcacgg cgatgacgac gcgcggcccg cgcatggcgc
    56761 ggcggttgtt gtggaccttg cggatgaagt ggcggagcat cgcctcggtg acgcggaagt
    56821 cggcgatgac gccgtctttc agcgggcgaa tggcggtgat gccgcccgga gtgcggccaa
    56881 gcatgcgctt ggcgtcatcc cccaccgcga cgacctcgtt ggtgccggtt ttgacggcca
    56941 caacggaggg ttcacgaagg acgatgccat gatctttgat gtaaacgagg gtgttggcgg
    57001 tgcccaggtc aataccgatg tcctgagcga caaaaccgaa aagtttgccg agcatgtggg
    57061 tgtgttaaaa ggtgggaaat tgggaaaaaa tcgcggacga acgccgtccc ggtggcggtt
    57121 ttccggatag gaatctcgcg gagagcccgc accggtcaga gaattcttga cgaacatgaa
    57181 gttaagggcg gggttttgga gggtcaaggg aagtttcctt cgcgggaaaa tgatctttgg
    57241 caagaggagg ccaagccgcc ttttgcgctt tttttccgac cggctttacc ggctcaatag
    57301 atcatggtgt tctccggcgc ggttgtctcc tcgatgctgg tggccttgag cacgggaacg
    57361 gtctgcgaac cgtccttttc atagcttacg atgccggtga ctttaaccca ggtcatgtct
    57421 ttgaagtcgg gggctttttg ctcgaactcc accggcacgg aatacggccg cgcgtccgcc
    57481 gcgcagcact gcaccagcat tcggaagatg cgcaggcggc ggccatcgtc gtttttcacc
    57541 ttttccggca gcacctgggc aatggtttcc accggctgcc cggtcagcac ggtctgcacc
    57601 tcgatgtcgc cgccggtgta gtagatctcg ggcacgtcga gcatgaagtt gccctccttg
    57661 ctttgcggca cctgctgttt gaggtcttcc agagtgaagc tgccgtagct ctgcggctgt
    57721 ttcccatccg cggcggcggc tgtttgcggg gcggaggcgg tggctgcggg tgttggtgcc
    57781 tccgctgtcg caggcttggc ggccgcgctt gtcgtgtcgg tgcgcaggga gaatttgtcc
    57841 gcgttggcgg tgctgttgta gttggggttg tagaggccct tgttgatgac ggcattggcg
    57901 ctgtaattgt ccggcgtctg aaaggctgcc caggtgatgg gcgcggcgag gatgaggatg
    57961 gcggtgacgc gcccgggcca tgagctttcc tccagcaggc cgtggccgtg ggaatgcccg
    58021 ccggcgtgat gatgctcgtg accgcatcct tcgtgagcgt gatgctcgtg gccgcatgag
    58081 gcaggcttct ccgcgtggtc atgcccgtgg cagcctggcg cgtgatcttc atgctcgtga
    58141 tggtgctcgt gatcgcacgg gccttcatgc gcgtgatgcg ggtgctcgtg atcgcacggg
    58201 ccttcgtgcg catgatgcgg gtggttgtga ccgcaggggc tggttttggc gtgggaatga
    58261 ccatgacagc agtcatcatg gccgtgattg tggtcatggc cgccatggtc gtgcccttcg
    58321 cagcccgcgt cctcggctcc cgccgtcagc agattgaaca gcgccagcac ggccagcccg
    58381 atgccggaga ccagcaccat gggccggaag atgccatcgc ccggcagata ctgcgcgatg
    58441 cgccccgtgg cgtaaaagta gagcagcacg gcgctccaca ggagcagcag ggcgacgctg
    58501 atgagatgga tggaaatgcg ggtggcgctc atggttttga ggaggtttgc aggagttcga
    58561 ccagtgatat ccaggggttg gaaaggaatg cgatgagaag gaaggtgccc agcagcatga
    58621 gcacgaccac gcggcggcgg aagacgaccg cgtacatgaa catgagcttg atgtccatca
    58681 tcggcccgaa gaccaggaag gccagcttcg ccgcctggga gaagacggcc atcggcgcgg
    58741 cgatgaaggc atccgacgtg ctgcagagcg acagcacata ggccagcgcc atgatcgagg
    58801 ggatggccag ccattcattg cccgccacgc tgtcgaggat ggcctggttc acctgagtgt
    58861 tgaagactga ggtgatgatg acgccgatgg tgaagtacat ccccgtgtcg aggaagtcgc
    58921 gcatggccgt gcgcatggcg tggaccagtt tgccgttgaa ggtcgtcttt ggcgcgtgcc
    58981 cgccggccct ggcgctctcg atgcccccca cgatgttcgc gttcagcacc tggccggggc
    59041 ggaagcgcag cacgatgagt cccacgatca ccgccaccgt gtagccgagc gagaggcgcg
    59101 cgatcgtcat tgtcgcattc cccaactcca tccatccggt caccgcctgg aattctttga
    59161 acgccgtcaa ggtgctgacc gccacgattg gattcatgat cggcgcggag agcatgtagg
    59221 tcagcgcgca ggaaagcggc agtcccttct gcaccagccg gcggatcacc ggcaccaccg
    59281 cgcactcgca tacgggaaag atgaggccga gaaaacccgc catgaaagtg gagaccacct
    59341 tgttgcgcgg cagcaggcgg tccagcagct tcgagggcag aaaggcgtca atcacacccg
    59401 acaacagcgt gccgatcatg atgtagggcg cgccttcgaa cagaatgctc aggaaggcca
    59461 tgagcacgtc tccaaggcgg ctcggttcgg gtagggtggc gaaaatcatt ggcggggtcg
    59521 aacccataaa cggcccgcgc cgcctgcttc aagcgggaaa gtgccgcatc ccttgcgcgc
    59581 ggcgcgtccg ttcgcgcggg gcagcgaccg ccgcgggggc gcccccgttt tgaaatctct
    59641 cgacaagccc gcccgtttcc ccttcactcg ccgcatcttc ccgcatgaaa gccacccgcc
    59701 tcctcgtcct gctcgccctc tccctcaccg ccgcccgcgc cgaaaactgg cccaactggc
    59761 gtggtcccgc ccagaatggc accagccccg agaccggtct gccggaaaaa ttcagccgca
    59821 ccgaaggcgt caagtgggcc gctgatgtgc ccggcatctc agcctcagtc cctgtcgttt
    59881 gggcggataa agtcttcctc acggctccca tcgctgaaga aaaaaaactc gtcggcctct
    59941 gctacgatgc caaaaccggc aaggaactct ggcgccgcgc catctccgag ggcggcctcc
    60001 agtgggacaa caagagcaac ctcgcctcac cctcgcccgt caccgatggc gagcgcgtca
    60061 tcttcctctt tgccgacgcc gtcgccgcct cctatgacct ctccggcaac ctgcaatggc
    60121 ggcgcgactt cacacaaagc cacggcgcct tcgccaccca gtggacctat ggcagcagcc
    60181 ccctgctcga cagcggacgc ctctacatcc aggtgctcca gcgcgatgaa gttttcgact
    60241 tccagggttt ccaaaaaggc acgcctggca aggacatgtc ctcatacgtc ctcgccctcg
    60301 atcccgccac cggtaaagat ctctggaaag tcgtgcgtcc ctccgaagcc gtcgccgaga
    60361 gcctggaagg cttttcctcg cccgtctgcc acgatgtcaa cggccagcgc cagctcctca
    60421 tcgtcggcgg cgactgcatc accggccacg acgccgccac tggcgccgaa ctctggcgct
    60481 ggggcacttg gaacaccgag aaaatcggcc actggcgcct tgtccccagc gtcgtcgccg
    60541 gagacggcat cgccctggcc tgcgcgccga aaaagaaccc cgtttatgcc gttaaacttg
    60601 gaggcaaagg gaccgccgaa cttgcctgga cctctgatcc caaagaggcc agcagcgatg
    60661 tctccacgcc cgccttccat gacggcaagt tttacatcct cgacagcgac cgccgcagtc
    60721 tttcctgtct ggaggccaaa accggcaggc tcatctggca gggagacctc ggcagcaagg
    60781 ccaagttcga aggcagcccc actgtcgccg atggcaaggt ctatatgacc aacttctggg
    60841 gccaggtcta tgtcgccaaa gccggcgggg acaaattcga gctgctcgcc gttaatgaaa
    60901 tgggggatgg cagcaagccc aacggcgacg ccagcagcgt tcgcagcagc atcgccgcgg
    60961 cgaatggcgc gctttacatc cgcacccagg acaaactctt ctgcgtgggt cgttaagcgg
    61021 cacctggccg gcgttggaac acggaaccct gattcaactc tccagtttgc aatcctccac
    61081 cgctccccag ccagtccttc tcagcgacat aggcaacgtc ctcgccttct ttgacttcgg
    61141 cattgccgcc aggcgcgtgg ccgggcgcag ccccttcccg gaggtggcgc tctccgcgcg
    61201 gctcgaccac atcaaaggcc ctttcgaaaa tggcgacatg gacgatgcca ccttcctccg
    61261 cgaggccgtc gccgcgctac agtttcaggg cagcgaggct gagttcgcag agatttggtg
    61321 cgacatcttc accgaaaaca ccgccatgcg ccgcactctc gatccgctgg ccggccgcct
    61381 gcccatgcgc ctgctctcca acaccagcgg cctgcacaag gagcacctgc tgagcgcgtt
    61441 cggcatcttc ctgcccttcg gcggcggcgt ctattcctac tccgcccggt gcagcaagcc
    61501 cggcgaggaa atcttccgca aaaccatcgc gcagctcggc cttgatcccg cgctgacttt
    61561 ttttgtggac gacctcgagc caaacatcgc caccgccgcg cgtctcggct tccacaccca
    61621 ccattatcag cccgcgcgcc acgccgcctt cgagcgcgac ttccatgcct ggctcgcccg
    61681 cgccgggatc agcgcgtagc cgttgaaaca cggcgcatca caaaagcatc atcaccggtg
    61741 ccaaaccggc ggcgcttggg cgtaaaaaat cgggcggcat accgtaatcc tccccccatg
    61801 agactgacca ccctgctgct cgtccccctg cttctctccc gcgcctggtc cgccacgccg
    61861 gtcaccgagg agatcaacat cctcgacctc atgaaaaagg gcggggtgga ataccacatc
    61921 aatcccgacc aggacttcgc cgatccgccg aatgagatct tccagctcaa gggcgaggag
    61981 ctgcacatca gcggccgcgg ctacggctac atggtcacga aggagagcta caaggactac
    62041 cacctcgtcg tggacttcaa atgggggccg aagacctggg gcaagcgcgc cgaccgctcg
    62101 cgcgacaacg gcatcctcgt ccattcctac ggcccgcacg gcgcttacgg gaacacctgg
    62161 atggccagca tcgaggcgca gatcatcgag ggcggcattg gcgacatcct cgtgctttcc
    62221 cccaagctgg ccgacggcac cgagctcatc accagcgtca ccgccgagat cgagctggac
    62281 cgcgacaaag agaagcgctg gaaaaaaggc gcgccgcgtc aggtcgtcac caaaggccgc
    62341 atcaactggg aaaaacgcca cgaagactgg gccgacaaac tcaacatccg ccatcccgac
    62401 gatcccgact cccccgtggg cgagtggaac cgcatggagg ttatcgccaa gggcgacaca
    62461 ctgcaatact tcgtcaatgg ccgcctcgtg aacgaagcct tcgactgcaa acccgccgag
    62521 ggccgcatct gcatccagac cgaaggcgcg gagatgatcg tgcgccgcta tgagctgcat
    62581 ccgctgggcg gcttcacgga aaaatggtcc gccatccagg ccagcggcgg cagcgacatc
    62641 agcgtgcgcc atgggcagga gaaagccctc acgcccgagg aatcgctcgc gcagatccag
    62701 ctcgacggtc cctatgaggc gcagctcgtc gccgccgagc cgctcgtgct cgatcctgtg
    62761 gaagtcacct gggatgataa aggccgcctt ttcgtcgctg aaatgcgcga ttacccgctc
    62821 ggtcccccga aggcaggtga tccgtggatg tcccgcatct cccagctcgt ggatgagaac
    62881 ggcgacggcc gcatggacaa gagcatcgtc tttgcggatc aactcgacaa cgtgcagggc
    62941 ctgctgccct acgatggcgg tctcatcgcc accacgcgca cccagattct ctttctcaaa
    63001 gacaccgatg gcgacggcaa ggccgacgtg aaccgcccgc tcatcaaggg cttcaaccct
    63061 cgccacagcc agctccaggt cagcgccccg cgctgggggc cggacggctg cgtgcatttc
    63121 aacaacggcc tcgacgccaa ggaaatctac cccgccgacg cgcctgacaa ggtcgtcggc
    63181 gtgcaaggtg ccaacttcaa atggaacccc aaaacgggcg agatcacacc caccggcggc
    63241 aagggccagt acggcggcgc cttcgacgat tacggccacc atttctactg ctcgaaccgc
    63301 aacccgctca tgttcaccgt catgccctac gaggtcatgc ggcgcaaccc gcacgccggc
    63361 atcacccagg tgcatgagga catcgccccg cccggagccg aaacacgcgt cttcccgctg
    63421 aagatcaccc acaccaccgc agacgcccat gccggcacaa acactgcttg ttccggcctg
    63481 ggcgtctatc gtggcgatct catgcccgag ctgcgcaaca acgtcttcgt gcccgacccc
    63541 accggccagc tcgtcacccg ttacaagatc gagcccaacg gcgcttccct caaagccacc
    63601 cgcgtcggcg agcgcaccga gttcttccgc agcggcgacg aatggagccg ccccgtgaac
    63661 ttcaccaccg gccccgacgg cgcgatctat gtctgcgaca tctaccgccg ctggattgac
    63721 cacgcccgct tcttccccga ggacttcgtc aaagccaacg acatgcgcca gggcgagcac
    63781 cagggccgca tctggcgcat tgtgcccgcg ggtgtccagg cttcagccgc tcagaaaagc
    63841 tctccagagc gcctaaaggc tggacacccg catcaccccg gccgctacca cgccatcccc
    63901 gaagccggct ggggagagca gcctgccgag cacaaagagc gcgcgctcct tttccgcatt
    63961 ttgcagaagc acgatgaagg ccaggtcagc aagccggagg aaatcagctt ccgcgccaaa
    64021 tgcatcgccg atcacccaga ggacgagtgg ctttcgaaag cggttttatc ttcctccgaa
    64081 tacaccgagg gccgtgtgct gaaagcggtc ttgcagtctc ccaatttcac cagccgcttc
    64141 tccccggccc ggtccgccac catccgggca tttgcggcgg gcgccatcgt ctcgggcgac
    64201 ccctctgacg tggctgaatg cctcagatgc ctcgaactag acaaagctga gcttctttgg
    64261 tggaaagctc ccttgctgca aggcgtgata gatggtttcg ccaaaggtta caacgtggag
    64321 cctaaatcac tcagtgcgtt catggcgctt ccgacaccag acctggaagc cagcagtcgt
    64381 ggcatgcttg atcttgaact cgctgaattt cagagcatcg ccaaagaaat cgcctccctc
    64441 cagtcccaga tagacaccat catcgccgac tcgaaagccc cgctcgatca acgcctcgcc
    64501 gtgctgcctt tgctcgcctc gcgcaaatgg gatgccgtgc agaccgtggt gaagagtctg
    64561 ctcaccaccg cgcagccgca ggagctgacc accgccacgg tggcgctttt gaaaaagttc
    64621 agcgccacca gcaccgcccc gctcatctat gagctgctgc cgactgccgg ccccgccttg
    64681 aagcgcgacc tcgtgcccat cctcaccggc aacgccacca ccgcgctcgc cttgttcaag
    64741 cgcatggacc agggcgagtt ccccaccgcc tgggtggatg tcgagacccg ctggcgctac
    64801 cagcgcggca agggcgagat ggccgacctc gccaaaaaac tcttcggcga ggccagcagc
    64861 gaccgcgccg ccgtggtgaa aaaatacatg gccgccacca ccctgaaggg cgactccgaa
    64921 aaaggccgcg ccgtctttgc cagcgtctgc atcacctgcc acaagcacgg cgacctcggc
    64981 gtggatgtcg gcccgccgct ctcggatgtg aaggtgaagc cccccgaggc cctgctcagc
    65041 gacatcctcg accccaaccg catgttcgag gcgcgctgga gcgcctacct cgttgaaacc
    65101 aaggacggcc gcaccctcag cggcctcatc caaaacgaga acaccgacgc catcgtgctc
    65161 gccatgatgg gcggcgccac cgagaccctc ccgcgcagcg ccatcaagac cctcaaaagc
    65221 ctggatcgca ccctcatgcc cgacggcctc gaagccgcca tcaccccgga gcagatgagc
    65281 gatcttctgg ccttccttgg cgggaagtga ggccagagcg ccagcttcgc gctcgcgact
    65341 ctccccgcgc gtccgatccg ggaagactca cgtcttcctc taccattgag aggaagactc
    65401 acgtcttcct ctacatgcgt cccggcgccc cgtagaggca aacgtcagtt tgccccggcc
    65461 cagggctgca agctggaagg ccgaatgtca gacctagatg ctgcggttcg tcttaactgc
    65521 accaccatga accgccgcgc cttccttcaa aagtcagccc tcgcgggcac tgccaccgct
    65581 ttgtcagcca aatcgtatgc ccaggtcgcc ggggcgaatg agcgggtgcg ggtggggatg
    65641 atcggctttg ggttgatcgg gcggattcac acacggagtt ttcatgggct gaaggacagc
    65701 tgccaggtcg tggcggtgtc ggattgtttt gcaccgcgca tggatgcgtg cgtggagctg
    65761 gtaggcggcg gatgcgcaaa gatgccggac ttcaggcgga tgctggagcg caaggacgtg
    65821 gacgccgtgg tcatttccac cggcgatcac tggcacgggc tgcaaacgat gatggcttgc
    65881 gatgcgggga aggatgtctt tgtcgagaag ccgctgcatc tcttcgttcg cgagggtgag
    65941 tggatgcaaa aggtggcggc gaggacgaag cgcgtcgttc aagtgggcac gcagcagcgc
    66001 agcgcggcgc attatcacaa ggcgaaggaa ctgctgcgcg gcggcgcgat cggtgacgtg
    66061 gtcagcgtgc agtgcaattt cttccgcaat gtgatgccgg gcatcggcag cccgccggat
    66121 ggcgatccgc ctgccggcct cgactgggac atgctgctcg gccccgcgcc gcagcggcgc
    66181 tacaatccga accgcgcgct gtatcacttc cgctggtttt gggactacag cggcgggcag
    66241 atgacgaatc tcggccatca ctcgctggac atcgtgcatt ggattttcga catcgaggct
    66301 ccaaaggccg tcagcagcag cggcggtcgc tatttcctca aagacaacgg cgaggtgccg
    66361 gacacacagg atgcgatcat cgagtatccg aagttcaacg ccatcgtgca gttccgcgag
    66421 tgcgccgcag gcgcgggagc gacagcgatg ggtgctctca ctttcatggg cacgaaaggc
    66481 acgatgacgc tgggacgcga tggctttgaa atcatcccgg acaagaagat cgagcccgtg
    66541 aatgcctttg cgcacatcat cggcggccat cccgtcggcg gtccgcagcc tgcgccggag
    66601 ccggaaggcg atttttggtg tgagaaggcc gaggacaagt ccggcgactg gaagcagcaa
    66661 tacatcgagc acgccaaaaa cttcctcgac tgcgtgaaga gccggaaaac acctcattcc
    66721 gacctcgcca gcagccactg ggtcagcacc acctgccatc tggcgaacat cagcgcctgc
    66781 acgggccgaa aagtcgtctg ggatgccgcc gcgaatgaca tccgagacga caaagaagcc
    66841 agcgccatgc tcacgcggcc gtatcgcgcg ccttgggatg cggaactgaa agcgttgatc
    66901 ggatgaatcg ccgtcatttc atcaccgccg cttctctagc tgtcgcacgg tcctttctga
    66961 gtgccgcagg tgctggcggg agtcgtttgg gcatctgcac gttttcgtgc catcaacgct
    67021 ggaagctctc caagccggtg gatgcggtgg agttttatcg ctacgcccgc gaactcggtg
    67081 cggaaggggt gcaagcgccg ctacgaggct gtgagcccaa aacgatccgt gatctggtcg
    67141 agcagagcgg cggctattat gagggcgaat tgcgtttgcc gaagtctgcg gccgatttgg
    67201 cggcttttga ggcggaagtg cgtcaaaccc gcgaagcagg cgcgacggtg gcgcgggcag
    67261 ttttcacaag tggaaggcgc tacgaggctt tttcgacgct ggaggccttc caaggcttcc
    67321 acgagcagtg caaacgcact ctggccctga tcgagccggt tttgaaaaag catcgtttgc
    67381 ggctcgccat cgaaaatcac aaggatcaca cgactgaaga gctggcgggc ctcatgaaag
    67441 cgatcagcag cgagtggatc ggcgtgctgg tggacacggg gaacaacctc gcgctgctgg
    67501 aggagccgca cgaggtcgtg gaggctctcg cgccgtatgc gctgagcgtg catttgaagg
    67561 acatggccgc gcaggcgagc gacgacggct tcctcctcag cgaagtgccg ctcggcagcg
    67621 gcttcctcga tctgccccgc atggtttccg tgctgcggaa ggcgaatccg ggcatcgtca
    67681 tcaatctgga aatggccacg cgtgacccgc tgcgcatccc ttgccgcacg gatgcctatt
    67741 tcaccacctt gcccggtcgc agggccacgc atctcgacgc ggcgcttcgc cgtgtgaagg
    67801 aaaatccacc gaaaggtccg gtgcctgccg tgagcggcaa atccagcgat aaggtgctcg
    67861 ccgacgagga gaggaacaac cgccacggcc tgcaatggat gaaacaatcg cttcactcat
    67921 gagccgttcg tttttccact tccttttacc ctgcttgccg ggcatcccgg tggttcctct
    67981 ggcagccgca gtttcctccc cgccttgctc gatcccaacc acgccgcgaa cgaaaccctc
    68041 ttccgggagc gccttggcaa cgaagtcgtg cgcgatggcc gctggaagct cgtgcgctgc
    68101 tgcaacgaca tccgcgacta caatccccaa accaaacgcg ggtccggcag cggcacacac
    68161 accgggggat ctctgtgtgc tccgtgtctc tggggttgat ccaatcggct tgagttcgcc
    68221 acagcgacac ggagcaactt cccgcggcat cactcgacac cctgcaccac cggcaggcag
    68281 ccgcgcacag aaggccgatg aatgcgcagg ctgggatcac tgccgatcca acccggctcg
    68341 ccccgcgcat tcacaaaagc caccccgatg ggaacctccg gcctcgtgtc cgccagccgg
    68401 gtgatctccg cctccgcccg gtctgacatc gcgatccacg cttttttcaa gacgatggga
    68461 tcgagctgac gggcctggat ggtttcaagc tggcgtggat tgatccgggc aaagcgccgc
    68521 atcatgtcca gcagcagcat ggggctgaag ccctcatcct ttccgcaggc ggcccaaatc
    68581 accgcctcca gcggataggt gcggtccagt tccacgatgt ccacatagtc gcgagtcacc
    68641 gagcgtgccg ccagcgccag cgccttgttg gcggccatgt caaagaggtg cagccgccag
    68701 ccgagcacat catctctttg aatcgggaag aaacgaaagg ctgagtccgc cgcccagtcg
    68761 atctccagtg cctcgccctc atgcgtcaga cgcgctttgc gaaaactcgt gtgggccgcc
    68821 caatcaccaa ccgcgtgctc caccgcataa ccagccgccc gcaaagctgc gacatccaga
    68881 tcgctggcac gcgccacctc ggactccgcc tcatgaaaaa tgtcgaagtc atggctgaag
    68941 cgcggcgaat catccgccga gttgagcacc agaccgccgg cgaaatggct ctcttcactg
    69001 cgattcgccg ccaaaacgcg cagcacttgt ttctggagag tcgttaaagg catgagttga
    69061 gttcctgagc ccgctgccag gcacggtggc cccctttgcg gagattcagc accacctcgc
    69121 gcacctcctc cacggtctgc ggagtgaagt cggggttcca ccaccaaaag cactcatgaa
    69181 agtcccgcac cgcctggcgc gccatttccg ccacctcggc ggagacgggc tggctggttg
    69241 tggctggagg ttcggtgttc atgcggcatc ctacgtccat gccaggaccg tctcaagcag
    69301 cgagatgcag gcccgccgta ggtgctggag catcaaaagt ccgtccttgc gccaccagtc
    69361 ccctgtcaga cccgctttgg cccgctcgtt ccagcctcca ccatgaaaaa gctgcttcct
    69421 ctcctgctcg ccgtctccgc tttcgccgcc aatgaatccc cggaggatgc gaggcttcgt
    69481 gaggcgattc cgtttggcgg tgacttcaaa ccaaatccga aagtcggcgc gtttgtcgtc
    69541 gtggggcatg gggcgcgcat tctcgtttcg aaggacgatg gcaagacgtg gacgcaggcc
    69601 ttctttgccg cgccgggggc ggatcacgga ccgtgggcca cgaaggcggt cgcttacacg
    69661 ggcggtgtgt tcgtcgtgcc cgtcggttgg ggcgcgccga cgatttatct ggcctctgat
    69721 gacgcggtga attggcgtca cctcaccaaa ggcaccgcga agctgccgga gggcagggga
    69781 gatgcgcgtg tgatgcccgg cacctggggc atcgcggcgg gaaagggcac cttcgtcggc
    69841 agcggctaca tgaccttctg cgccacgagc gatctcgggc agagtttcag ttccttcggc
    69901 atgtggggtg cgttcaaaga cgatccgcga ggcaaactga gcacgcatca cgtcggcgcg
    69961 gtgtattgcg gcgatgccag cgggcgcttc ctcgcgctcg gtgacaatcg cggcgactcc
    70021 gccgcgaagt ttggcaacct cttcgccagc gatgacctcg gcaagacctg gaaatggctc
    70081 gcgccgaagg gtctcgatgc ctccaccggt cgcggtgaaa tgctcagcaa cggccgcctg
    70141 ctcgtcatga ccgacaaaga cgccgcgaac gcctggacca gcaccgacgc gggcgaaacc
    70201 tggaccggcc cgcacgccac cggcacgcaa cgcgccacct tgagcgtcgt gggcagcgag
    70261 ttctggctct gcggcaaaaa atcccgcgcg agcaccgacg gtcaaacctg gcgcgacctc
    70321 cccgccgccg tccccgaagg caaaatcatc gccagcgacc aaggaacgct catcagcatc
    70381 caccaacagc gcaccaacat ccaacgcagc actgacggcg gcaaaacctg gcagcaagtg
    70441 catcaataca ctccacccga cttcaaaggc ggtgctcaag gcctgcgtgc aggcgcgtgg
    70501 ggcttggtgg ctccgtgaag gttgctttaa acgggcggaa tttggctttg aagtgtcatt
    70561 ctagcggaat caaactgacg agactccaca aggaacatca cttcatgaaa tctctgctgt
    70621 ttgtcctccc gcttcttgtg gcaacgcaac gagtgaaggc tatcgatccc tttttcccgc
    70681 ctgatgactg gaatgcttat cagcagcgca tcatcaaaga tcgcgaacat gaaaagccgg
    70741 gctggctgcg gcctgctccc ggcacgagtc cagccgtagc gattcagact ctgattttgc
    70801 cagagtttaa ggtggagaat gtttctcttg aggttgcctt ccaagcctgg caggacgcct
    70861 gccgtcaggg cggcatcaat gtgcgtgtgt atctgggtcc tgcggccttg atcaacaggc
    70921 cttccatcac gcattcggca aagggcgaga cagcagcacg aactctggca tttattgaga
    70981 acttgggact ggctcgggta aggctgctag aggaccactt tcttgctgga gccaactccc
    71041 ttgatcctca tgaactgcgc ttccgcgtct gggtgttgag cgagatggcg gcaggctttc
    71101 tcgatctgca caaactgaag ccggcacaga caggttggtg ctctgctgac tccgcgttga
    71161 cccaaatcgg ggcagcattc ccgcggggtg cctatgccga ctatcacgaa cagatgcgtg
    71221 cgctggtagc catcaacaca accgtgacac tggatgcctt gtcggagtgg attgaagaaa
    71281 tggaggcacg cgccatcatc cagcagttca aagacaagga agaggttcag atggacggct
    71341 accgtctggc ggttcgatcc cagcaactcg atgctcgcaa tgcggccaaa attgccgcgc
    71401 ggctgcggga cgcgaaaggc aggctcgttc cgttcgcggc aagctcgcaa acctgggcca
    71461 aatcaagggg gctcatcaca cccgcgggca gcgcagtgtg gttggatgtg gcgtcttcta
    71521 ctttgtggct gcgcagtcgc ccggaatctt tggaccagtt catgccacag cttcaaaagg
    71581 ccaaccttga gaggtagaag cccgcagaca ctcgtcgcat gaaaactctc ctcgccgccc
    71641 tcctttcctc attcctcatt ctggtttcgt cattcgcggc acagccgcgc ccgaacatcc
    71701 tcttcatcct cacggatgac caaggctggg ccacgctcgg ctgctatggc tcgaaaaaag
    71761 tgcccgcgcc gaatctggac cggctggcgg gcgagggtgt gcgattcacg gatgcttatc
    71821 tgccgccgca gtgcgcgccg acgcgcgcgg gggcgcgaaa gtttgcgacc gggtcaatat
    71881 ccctgagctg ttgagcggcg tccagaaggg gcttttggtg ccggtgcatg cggttgccca
    71941 ggcagtagga acaggctccg cggcagcggc aggaggcgtc aaacgcgcgg ctttttcgat
    72001 agggctggcg gcgggtgcgg ctcattgcgg gaaagcatcc gcaaaacaga aattgccgcc
    72061 agtgcgcaga tggcagatcc aaagagaagg tgtttgcctg gcgcgatgcc gctttttttg
    72121 ccattgagct gccaccgcca ccccgcgcct gtgccctggc tttccggtga aaaacgcacc
    72181 gtccaggatg gccgcgccgt tttcccgctg gaaatggggg gagtccgccg cttagtgccc
    72241 gcacatcccg ccgccgccat gccgaacgaa aagcccgtct ccctcaaaca cctgaccctg
    72301 ctgggtaaag aaaacattcc gcaagcgggc ggatacctga tcctgccggc ccggctcgat
    72361 tacctggacg tgctgaggct ggaggcggtg ctggagggcc ggaacatcgt ctacctctgc
    72421 gagcagggcg cggccctgca ccccatgctg cgcgcgcatc tggagcggga gggcgcgcag
    72481 gtgctggtca tctccccgga tctgacggac accgccgcgc tgcgcaaggc cgtgaccggg
    72541 aagaccgcgc aggacgcggt catcatcttc ctgcccgcgc aggcggcctg cctgaacgcg
    72601 ccgctgtgca ccatccccgg catgcgcctg gagttcctgc tcaaggcggg ctgccccgtg
    72661 ctgccgctgc atgtgctgca cccgcgggag gtggcgctgg acatcggccc gcgcccggac
    72721 gagaccgccg tggtaatgag cttcggcatg ctgctcactg gagaggatgt gtccctggcc
    72781 tcctaccagg aaagcctgct ggagctgtgc cggcaggctt ttgaaaccca ccccgcactg
    72841 cctctgcatc tggcctgcgc gctgatccag ggcttcaagc gccacgggcg gaacaatgtg
    72901 gtggtggacg gcaaggacga caaggagatg ggcttcgaca cgatcttcgc cgccgccatc
    72961 gcgctctcga gggtgatcaa aagcgagacc gcaaaggagc gtgtcggcat cgtgctgccg
    73021 cccggactgg gcggcctcat cgccaacgtg gcggtgctgc tggcgggcaa aatcccggtg
    73081 aacctcaact tcaccgccgg gcgggctgcc atcgagttcg cgatgaaggc ctccggcctc
    73141 gaccgcctcg tcaccgccga tcccgtggtg cgcaagatgc agaccttccc ctggccgccg
    73201 acgcgccagc tcatcctcgt ggagcgcctg ctgccgcgga tgaaaacaaa gatcggcctc
    73261 tggctggcgc tctccaagct gctgcccgcg tctctcctgg ccgcgctgct cggcgtgccc
    73321 aggcatggcg gtgacaagga ggcgctgctg cttttcacca gcggcagctc cggcaacccc
    73381 aagggggtgg tgctcaccca ccgcaacatc atggccaacg tcatccagtt cggctcgcgg
    73441 ctgggcctca agtcggacga cgccattctc ggcagcctgc cgctttttca cagcttcggc
    73501 tgcacggtca cgctctggta tcccgtcatc gccggcgtgg acctcgtcac ttaccccagc
    73561 ccgctggaga cgaagaggct ggcggaactg gtggagaagc acagcgtcag cgtcatgctg
    73621 gccacgccca cctttctgcg cggctggctg cgcggcgtgc cgcgcgagtc gctcaccagc
    73681 ctcaagatgg tggtcaccgg agcggagaag ctgcccgcca ccgtggcgga ggcctttgag
    73741 gcgcgcttcg gcaagcgcgt ctatgagggc tacggcctga ccgagacctc cccggtctcc
    73801 aacgtcaacc tgcccgaccc cgtgcccctg gccgaggagg gccacgcctg gctgcccgcg
    73861 caccggcctg gcagcgtcgg ccagctcctg cccggcctgg ccgtgcgcat caccgagccg
    73921 gagggcgggc gccgcctctc cgtccaccag agcggcatga tctggttcaa aggcgccaac
    73981 gtcttccaca gctaccacaa cgaccccaag cgcaccgccg atgtcaagga cgaggacggc
    74041 tggttccgca cgggcgacat cggccgcctt gacctggacg gcttccttta catcgagggc
    74101 cggctcagcc gcttttccaa gatcggcggc gagatggtgc cgcacgagac cgtggaggag
    74161 gccatcatca aggccatggg cctggagaac gagtccgcgc gcaagatcgc cgtcgtcggc
    74221 gtgcccgacc ccgacaaggg cgaggcgctc atcctcatct cggccatgac cggcggcccg
    74281 gagaaccagg agatacttga cctgcgctac cgcctcctgg agaagggcct gccgccattg
    74341 tggatcccga agaaaatgat ccgcgtcagt gacatcccca tcctctcctc cggcaagctc
    74401 gacgtgcaaa gctgcgagaa gctcgcgcgc ggtgaggtgt gaggcttgaa gcgcgcaatc
    74461 cgaggcggcc cgatgaagct catgctggaa acctggcgct cctcacccgc ctcggcgcat
    74521 gtggcgccgt tcgcggtgtt catgaccttt ctggccgtgc cgggctggtt ccgcgtggaa
    74581 aatcccgagc tgccctggca tgtgcgcgcg ccggagctgt gggtgtatcc tttgcagacc
    74641 ctcgtttgcg cggggatgct gtttttcttt cgccgccact atgcgctgag gccgttgcgc
    74701 gggctggggc tggcctgcgt gctcggcgtg gcgggcatcg cgctgtggat tctgccggcc
    74761 tgggcatacc aaaagctcgg cactgaaaat ccgccgatgt ggtgggcctg gatcgggatg
    74821 gcggaccgga gccagggctt cgatcccggc cagctcgccg ggtggcctgt gtgggagcgg
    74881 atcacgatct tcatgcgctt cgcgcgcctc gtcatcgtgg tgccgctggt ggaggagatt
    74941 ttctggcgcg gctttctcat gcgctggctg gcggcggagg ggcggccctg gcatgagacg
    75001 ccctttggcg cgcacacctg gcggagtttt tggatcgtca ccacgctggt catgctggcg
    75061 catcagcccg ccgactggcc cgccgctttt gtctggggcg cgctggttta tctcgcggcc
    75121 gcgcgcaccc gcagcctggg cgcctgcgtg ctcatgcacg cgctggccaa cgcgctgctc
    75181 ggagcctggg tgatgggcac caaacaatgg ggattctggt gattgtggac tcgccgcaac
    75241 cggaaggaca tttggtttga aatgccagtt gcagccaggg gtctgtcatc catcttcgcc
    75301 cccctcccga atgtccaagc ctgtcaaagt cgccgtcctt ggtgcgagcg gatactccgg
    75361 catcgagctg ctgagaatcc tcctgcgcca tccccatgcc gaactcgtcg cggtcacttc
    75421 ccgcacgctc gcgggcaagg cgctgtccgc cgagtttccg cgcttccgca aggtgggcgt
    75481 ggccgacaca ctggtcttca ccgagccgga cgtggacgct ttaaaggcga gcggagcgca
    75541 ggccgccttc ctggcgctgc cgcatggcgt gtccgcggag tatgccgcgc cgctgctgga
    75601 ggcgggaatg cgcgtcattg acctcagcgc cgacttccgc ctgcgcagcg cggaggttta
    75661 caaggagttt tacgcgcacg agcatcccgc gcccggcctg ctcgccaagg ccgtctatgc
    75721 gctgccggag tggcgcgcgg aggagatcaa aaacgcgcgc ctcatcgcct gccccggctg
    75781 ctatcccacc agcatcctgc tgccattgtt gccgctgctc aaagccggcc tgctctccga
    75841 ggatccactg gccgtctcca gcatgagcgg cgccagcggc gcgggccgca atgccagcgt
    75901 gccgctgctt ttttgcgagg tgcagaacag cctgcgcgcc tacagcgtgc cctcccaccg
    75961 ccatttgagc gagattacgc aggagctgtc cctcgccgcc gggcgggagg tgcggctcac
    76021 cttcgtgccg catctcgtgc cggtcttcgc cggcatctgc accaccacct ttgccgcacc
    76081 cgcgtccggc gtgtcgctcg accaggtcga gtccgccctg cgcgccgcct acgacgggca
    76141 gcccttcatc cggctgctcg gcaggaatca atgccccgac accaagcatg tcatgggcac
    76201 aaacttcgtg gacatcggct gggcgctcga cgagcgcgcg ggccgcctcg tgctcatgag
    76261 cgccgaggac aacatcggca aaggcgcctc cggccaggcg gtgcagaacc tcaacctggc
    76321 ctgcggcttc gcgccggacg cgggccttct gaacgcatga gcacgacgac catggattgc
    76381 gatccgttcc gcgaaatacg cgtgcctttc aaggtgatca aaggcggtgt caccgcgccc
    76441 aggggcttcc gcgccgccgc cgtctcctgc ggcatcaaaa accccgaggc cgcccgcctc
    76501 gacttggcgc tcatcgtctc cgacaccccc gccgtcaccg acgcggtcta taccaccaac
    76561 aaagtgcgcg ccgcctgtgt gcgcgtctcg cagatccaca tccgcgagag cgacacgcgc
    76621 gccatcatcg ccaacagcgg caacgccaac gcctgcaccg gcccgcaggg catccaggat
    76681 gccaaagcca tgtgcaaggc cgccgccgag gcgctcggcc tgcgcatgag gcaggtgcag
    76741 gtatgctcca ccggcatcat cggcatgaac atgcccatcc agcgcgtcac cccgcgcatc
    76801 ccggagctgg cggcaaagct cagccccacg ggctccgacg acgcagccgc ggccatcatg
    76861 accagcgaca ccaagccgaa gtccttcgcc atcgaggtgc cctgtccggg aggcggctcc
    76921 ttccgcatcg gcggcatcgc caaaggcgcg ggcatgatct gcccggacat ggccaccatg
    76981 ctttgcttca tcaccaccga cgccaaaatc agcaaggacg agatcaagcg cgccatgcgc
    77041 tgcgccgtgg accagtcttt caactgcatc accatagacg gcgacactag caccaacgac
    77101 accgtcatcg tcatgagcaa cggccaggcc tccgcgcccg ccatgaggaa aggctctgac
    77161 gaggccaaaa tcttccgctg cgccctgcac aaggtcatgc tcgagctggc gaagatgatc
    77221 gtcaccgacg gcgagcgcgt caccaagttc gtcgaaatcc gcgtgcgcaa cgcgcgcacc
    77281 catgccgatg cgaaaaaggt ggccgaagcc gtcgccaagt ccatgctggt gaagtgctcc
    77341 ttccacggca gcgacccgaa ctggggccgc atcatccacg ccgtcggcta ctccggcgcg
    77401 cgcatccgcg aggagatggt ggacatctat ttcggcggcc tgctcgcctg caaaggtggc
    77461 ctcacctcgg gcacgcctgt cgccgagttg gaaaaagtcg tcaaagaacc gcgcttctcc
    77521 gtcaccattg acctcaacct cggcagcgcc aaccacatcg tttataccag cgatctctcc
    77581 gaggcctatg tggacttcaa cagcagcgaa tactccgccg ccgtccacgc ccgccgccag
    77641 aaggggctgg cttgaggcgc ggcggcttca tgtccgccgg tgtcatcccg atcagtcaat
    77701 gcccgcgcga caagtgcatg gagcacgcgc gcccccgcgt gttccggggc gcttccgtcc
    77761 gcgccttcct ctccctcaga tgaaataagc ccgcctcggt gacgttttcg cagcactcat
    77821 gatttcccgc agaaaactcc tccgacaaat cgccgccacg gctggcgcgg ctttgggttc
    77881 acccttgttg ccaaacatca gccaggcggc atcccgtgcg cacggcggcg gaccgaagcg
    77941 cgtgatcttt ttcctgcaaa accagggctt tgatccggcc acctgcgttc cggcgggcat
    78001 gaaaaacagc ggctcgctgg ccagggcgaa gcttcccgag cccatcgagg cgctggagcc
    78061 tttcaaggag cggctgcaca tcatcaacgg cctgcacggc ctgcacacca gcccgtcgca
    78121 cagcgcgttc ttcggcgcgc tcggcggtta tcgcggcagc gatggcgtgc cgcccagcgc
    78181 gtccacgatt gactatgaac tgagcaaggt gctgcccgag acgctgctgc cgcatctctg
    78241 catcggcatg gactcgatgg agaacatgag ggccaagccc accgtcgcga acctgtcggc
    78301 cagcggcgcg ggccagccga tcttcatgca ttcgaacccg aaccacctct accagatgct
    78361 ctacggcggc atctcggacg gggacatccg caagcagcat gaggcgcggt cgagcatgtt
    78421 cgaccgcatc gagcagctcg ccgccgcgca cggcaaggcg ctgccgatgg cagaccagca
    78481 gcgctacggg cagtacgttc agggtttcca ggacatcaac ggcctgcggt cacgcctcaa
    78541 cggcgtctcc gatcatctgc ggaactttgc gccaaaggtc gatgaccgtt acacgaagcc
    78601 cgagttcgaa accgactggc acgacgtgct gctcgacctc ggcatctcgg cgatcaaatc
    78661 cggcatcacc agcgtgctga ccatcggctc cggtcgtggt gaaatcttcg gctcgtggaa
    78721 aggcctcggc gtcgaggagc agggccacaa tctcggccac atgaagcagc cggacaatcc
    78781 gatctggatc aagatccggc aatacaacag ccgcatgctc gtgaagctca tggaagagct
    78841 ggaaagcgtg cccgaaggca gcggcacgat gatggacaac acgctcatcg tctataccag
    78901 caacaacgcc gacgcgcagc acaccagcgg cgccacctgg ccggtcatgc tgcttggcaa
    78961 ttgcggcggc attttcaaaa ccggctgctt cacgcagctc gacggcaccc gtccgatcaa
    79021 cgcgctctac gcgacgatcc tgaaaactgc cggcgtcgag tgcgagcgct tcaacatgag
    79081 cgagaagatg gccgcgaagt tcgactccgg caccggcccg ctccgagagg tcctggtgta
    79141 aggcaagccc gccgcttcgc cgggcacgtc cgcgagcgac tgccgaggcg gcttcagcag
    79201 cagacattga ccagcctgtg gtgaaccagc atccagaatc agaccgcgtg gagccggggc
    79261 ggcgcgcgga atctcatccg gcgggcagtg ggagaaagtc gcggccggta gcgaggctct
    79321 cgacgtcttc atgaagtgcg tcaccattct tctccaacgt catggaactg tggcagcata
    79381 gcccgcgacg atgacctcca ccccgcatcc aacttccgcg aggctccttt tcacagcgcc
    79441 tccgccaaat gcggactttt gccttcacat cgctggaata acatccaacc ttgaccatct
    79501 catgatgtcg gcgagtcttg ccgccgctgt cccaatgaaa cgcttctggc aaatcctcgc
    79561 acttctgatg ctggccttga tggtcccggc ttcgatgtgt tgctatgggg cggatggatg
    79621 caaggcggac gtttgctgct ccgaggaaag ccacgagcat gaagaccacg aaggaggcga
    79681 acatcacgct cccgtctcct gcccgagcga tacgatctcc cacagccagg ttcccgcgcc
    79741 ggtcacgatg ccggagatgc agatggtgga gctggcggag ttgattcagg cgatgatccg
    79801 tcttcaagac gatctcgcca cggccacgga gtcttccggg ctgccgatga ccaccgcgcc
    79861 gccggagttg cgagcgacgt ggcatttcac gcaacgcacg gcgctgcccg tgcgtgcgcc
    79921 ttcgatccag gcttaaagtt ccgtgcgttg gtccgcccat gaaggcggtg acatccacgc
    79981 aggagcttcc caccgcgacc gctcgcagag cggcatcgtg gctttgccgt gcctccgtcg
    80041 aaactcaacc cccatctcat catgcgctta ttcatccttt catctctgct gctcgctgct
    80101 gtgccgcagc ttctcgcggc ggattccaaa ccggagccgt cttccatctc cagtctcgtg
    80161 ggccgcacgc tcgcggggaa tcctgaaatc cgcttctatg aggcggaggt cgcagcggcg
    80221 aaggcgactc gcagcacggc gggcaggcag tcgaacccgg agctgagcct gcaagtcggc
    80281 aacaaccaca tccgcaacgc cgacagccgc tcgaacggcc tagccttctc cgccgagctg
    80341 gcgcagccca tcgagtggcc gggccgcttg ggtttgcgca aggccatcgc gaaccgcgac
    80401 atcgctatgg cggagctggg gctggagcgg tttcgctttc atctcgccgc ccgtgtccgc
    80461 gtgctggcct tcacgttgtc agcgcagcaa gatctcgcca atgccgcgaa tgaagtcgcc
    80521 gcacgctatg cctcactgcg cgaggtgatg gtgcaacgcg agccagcggg catcgctcct
    80581 cagcttgaga tcgctaccat tgaggcagcg gcgctcgtgg cagagtcgcg cgcagcgaaa
    80641 gctggtgtcg tagtgcaaaa ggcgctgctg gagctgaacc aactcatggg ccgccgtgcc
    80701 gacgcgccgc tcatcgtgaa gcgcggcccg ctggatgcca aggccgcgcc tgcgctgctc
    80761 tccctcctcg gctccgcaat gaagaaccat tacgagttgc gcctgcgccg tgccgagctg
    80821 gagcagcagg gcttcaaggt cgagctggca aagaacgagc gctatcccgc cttcaccatc
    80881 ggccccttcg tcgaggtgca aaacacccgc acgctggatg accagaccgt ggcaggcatc
    80941 ggcatctcct tcccgctgcc gttttggaag aatggcaaag cggaagtctc caatgccgag
    81001 gcacgccaga tgcaggcaca ggccacgctc gccgccgcgc agcgcgaggt ggagcggcag
    81061 gtcaccgaga gcatgctcat ctaccagacg cagcaggcac gccttgccgt gtggaagggc
    81121 gatgccgtca cgaagttcag cgatgccgcc gcgctagcgg acaggcattt cagactcggt
    81181 gccgtgccca tgagcacctt tgtcgagctt caggacaagt atctcgaagc cgtcgaggcc
    81241 atccacagca cgcaaaccga ggcgctggag gccgcgctca cacttgaaga actcaccggc
    81301 gcacccggca gcctcatcac caccaaaaac taacttcacc tcatcatgaa actcttgctc
    81361 atcctgtctc tcatcgtcac cgccgccgcg ctcaccagtt gcagcgaatc tagcgccagt
    81421 ccttccactg aacacaccgc cgcaccggaa aacggcgcac agttcaagga aggcaaaggc
    81481 gtggccctca ccgcgctcat ggccgagtcc atccgcctgc aaaccgccga ggtgcaggag
    81541 gaaaagatcg cccccgcctt caccctcagc ttgcaggcca tgcagcgcgg caccgaggcc
    81601 agcggatggc ttaccgccga tcaagcggcg cgtgtgcagc ccggcatgga ggtcgagctt
    81661 ggcaccgcga aaggccatgt gctccgcatt gaaaaggcag cctatgccac gcttggcgac
    81721 tttgaagtca ccgtgcaaac tgccgccccc atcgaggcag gcagcgtcat caccgccacc
    81781 ttccgcttcc ctgctggcga tgccgtcacc gccattccac aggccgcgct gctcaccacg
    81841 gctgagggca ccttcgtcta tgccaagaac gacgagttct tcctccgcac gcccgtcaaa
    81901 gtcggcgcac gcaacggcga tcatgtggag atcaccgatg gcctctacac tggcgacgag
    81961 atcgttacca cgcccgtcat gtccctctgg ctcgccgaac tccaagtgct gcgcggcggc
    82021 aaagcctgca cctgcggcca ctaaacccca gccatgttct ccatcgccat ccatcaccgc
    82081 cagcagcgcc gcatgatcac ctggatcatg ctcgtcgtgc tgctgccgtt gtttgcggtg
    82141 ccgtggagtc aggcgcatca ggggcagtgc tcacctcgga tgcccgcgtg tgagatgacc
    82201 tgctgcaaag acatgtcgtc agacaacacc acgcatcact ccgagtgccc ctgccaggac
    82261 accgaccaag gctgcgactg cgcctgctgc ctgcacatcg cgtccaccat gcctccggtg
    82321 gtgatgagcg ctccacagac ctgcccgctt aatgctcata gcattcggcc atggtcgcag
    82381 cgtgcccctt ctcgcaccga gcggcctcct ttgccgcctc caaaacaggc gcaccacttc
    82441 caccccgtcg gcactacgcc gacttcagtt cattcaaacc attcaaactt aacttatcta
    82501 tgaaaacatt gtttctgtct cttgccctca tcctgaccct caccggtctc tacgcagcca
    82561 ctcaatccag cacggacgcc aagtccaact actgccttgc cgctgcgtgc tgcaccggtg
    82621 gcgcttgctg cgccacgggt gactgctgct gcctcaccgg cagttgctgt gaggcgggtc
    82681 tctgctcctg cgacccaggc tgctgcaccg gctgcgagtg ctgcgctgac taattagcgc
    82741 ccccacggcg tccgccgact ccggtcggcg gacgctcttt tcttcgctct catgctcacc
    82801 catctcatca ccttcgccat gcggcagcgg gccgctgtgc tgcttgccac gttcatcttg
    82861 atcggcgtcg gatcatggtc ggccatccat ctgccgattg atgccgtgcc tgacatcacg
    82921 aatccgcagg tgcagatcaa caccgccgtc aatgcgctcg cacccgagga gatcgaaaag
    82981 ctcgtctcct tcccgattga aagcgagatg gcgggcctgc cgcagatggt agaactgcgc
    83041 tcgctgtcca agttcggcct ctcgcaggtc acgatggttt tccatgacga tgtggacctc
    83101 taccgcacgc gtcagctcgt caccgagcgg ctgcaaaccg tgcttgatga actcccgccc
    83161 ggcctctcac caaagctcgc gcccgttgcc accggcctcg gcgaaatctt ctactacagc
    83221 ctcgactaca gcgccgacgc cgccaacaag cccgccactc gtgaggcaca actcatggcc
    83281 ctcgcgcaaa tccaggaata ccaagtgaag ccattgctgc gcggcacgcc cggcgtcgcg
    83341 gaggtgaata ccagcggcgg ttatcagcgg cagatcgtca ttcagcctga tcccgcacgc
    83401 ctcgccgcac gcggcatgtc gctcgacatg ctcgcggaga tcgtggagca aaacacccgc
    83461 aacgcaggtg gcggctacgt cgagatcggt ggcgagcagc tcatcgtccg cgccaccagc
    83521 cgcgtgaatg acatcgagca aatcctcgcc atcccgctca agttcgcagg cggtgtgaag
    83581 cccatcctgc tcagcgaagt cgccaccgtc agcatcggca gcgccttccg cacgggtgca
    83641 tcgactgatg acggcaaaga agccctcatc ggtgccacca tcatgctcgc gggagagaac
    83701 tcgcgcctcg tcgcccaggc cgtgcgggcc aagctcaccg agattcagcc caagctccca
    83761 cagggcgtca tcatccgtgc cttgtatgac cgcagtgatc tcgtcagtcg caccatcgcc
    83821 accgtggaga agaacttggc cgagggcgca ttgctcgtcg tcgtcgtgct ctttgcgttg
    83881 ttgggaaact tccgcgccgc cgccatcgtc gccctcgtca tcccgctttc catgctcttt
    83941 gccatgaccg gcatggtgcg ctatcacatc agcgggaact tgatgagcct cggagccatc
    84001 gacttcggcc tcatcattga tggtgcggtc gtcatggtgg aaaacatcgt gcgtcatctc
    84061 ggcgagcgac agcacgcgct gggccgcagg ctcaccgtca gcgagcgcac ggaagaagtg
    84121 ctcaaatccg ccaaagaagt cgccaacccc atgttcttcg gcgtgctcat catcaccgtc
    84181 gtctatgtgc ccatcctcgc gctgcaaggc atcgaaggga agatgttcaa gcccatggcg
    84241 ctcgtcgtca tgctcgcgct cggcggttcg cttgtgctcg ctgtcacctt gatgcccgtg
    84301 ctgtgctcct atttgctcgg tggcaagatt caggagaaag acaactggct cgtcaccctc
    84361 acgaagcgcc tttacacccc gctgctcaag ttcggcctgc ggttcaagcc actcatcgtg
    84421 ctccccatga tcgtgctctt tggctcctcg ctctgggtct tcacacgtct cggctccgag
    84481 ttcatcccgc agctcgacga gggcgacttc gccttccagc tcatccgcag cagcagcgcc
    84541 ggtttgtctt cctcgcttga tttgcaaaag caaagcgagg ccgtcatccg ccgcgacttc
    84601 cccgaggtga aagagatgtt ctcccgcatc ggcaccgccg agatcgccac cgatcccatg
    84661 aaccccaacg tggccgacac ctacctcatg ctcaccccgc gtgatcagtg gcggcaggag
    84721 aatggcaagc ccattagcaa agagcacctc ggcgaactca tgcgcaaagc gctgctcgat
    84781 caagtccccg gccagaacat cctcgtcacc cagcccatcc agatgcgttt caacgagatc
    84841 atggccggag cacgcgccga cctcgtgtgc aaaatctttg gcgacagcta cgacgaactc
    84901 gaacgcctcg ctggagaagc ccgcaccatc atcagcggca tccgtggcgg cagcgagacc
    84961 gagttcgaca acatcggcaa gaaccccatg atcgaaatcc agcccgaccg cgaggccatg
    85021 cgcaagttca acgtccacgc cgatgaactc aaccgcctca tcgaaaccgc tctcgcggga
    85081 accgaagtcg gcctcgtcat tgaaggcaac aaacgcagcc ccatcatcgt ccgtctgggc
    85141 gaagaccgcc gcagcgacct cgaaggcatc aaacgcctcg ccctccgcac cgaagacggc
    85201 ggcatgttag ccctcggcca agtggcgaag atcgtcctcg tgccccagcc cgtgcagatc
    85261 gcgcgcgaag acgcgcagcg ccgcgtcagc atcctcatca acgtccgtgg tcgcgatacc
    85321 gaaggcttcg tgcaggaggc cacgcaagcc attcacgaca aagtgaagtt ccccgacggc
    85381 tactacttcg agttcggcgg ccagttcaag aacctccagg aagccaaggc ccgcctcatg
    85441 atcgtcgtgc cgctcgcgct cgccttgatc ttcgtgctca tcttcctcag cttcagctcg
    85501 ttccggcagg ccacgctcat cttcatgtgc gtgccgcttg ccgtcactgg cggcatcttc
    85561 gccctgcatc tacgcggcat gcccttcacc atcagcgccg ccgtcggctt catcgcgctg
    85621 agcggcatcg ctgtcttgaa cggcatcatg ctcatcagct acatcaacca actccgcacc
    85681 gaaggccgcg aagtgcgcga ggccgtcgtc gaaggcaccc tcacccgcct gcggcccaag
    85741 ctcatgaccg cgctcgtcgc cagcctcggc ttcgtcccga tggccatcgc caccggagcc
    85801 ggtgcggaag tccagcgccc catcgccacc gtcgtcatcg gcggcatcct cacctccact
    85861 ttcctcaccc tgctcgtcgt gcctgtgctc tacgagtgga tcgaacggaa aaccaaacca
    85921 ccaacaaaca caccatgaaa acactgctca acatcctcat catcgccgcc ctcgccacca
    85981 cgtccgcctt tgccgacgcc aaagtcaaag ccggtccccg caaaggcctc atcctcgatc
    86041 tcggcggcaa acaagccgag ttctttgtcg aaaaagaccg cagcatcacc atcgccgtct
    86101 atgacgccgc gatgaaagcc cagcccgcca gcaccgaagt catcaccgcc accgccgaag
    86161 cccccagcgg caaagtgaaa atggagttcg cgaacaaaga cggcaaactc gtctccacca
    86221 ccaagctccc cgaaggcgaa ggttatcaag tcgtcgtcca agcccaagcc gcccctgatg
    86281 ccaaaaagaa aaacttccgc atcaagctcc tcctctacac ctgcaaagag tgcagcaacc
    86341 ccgaatacgc ctgcacctgc gccgatcatt gagatcgaaa cccatggccc gtcatgcccg
    86401 gaaatgacac ccacgacgcc cacgattacg ccgagcgcat ccgcgctcgg ctcccgaggc
    86461 tcttgcaggt cctccgggaa cgctgtgggc tgagtaaatt tgccctcgct cgtgaaagcg
    86521 gcgtcagccg ggaatacatc ggcaatttcg aacgtggcaa agccagtcac catgcgggat
    86581 gtgctcaaca agctcgacga actgaccaat gcgctgcgga ggggagaact cgcggcctac
    86641 gatcacgcgt ctgttcaggc tcaggatccc gccaaagaag gtgaagatgt gcttgtttgg
    86701 ggcgttttcg ccaagcggtc caaatccccc atgagcactg ccctgaatcg aacccatttt
    86761 ctcgcgtgag ccgtcaccgt tttgccctca ttgcggctgc gggggtcgct ctttgctcct
    86821 tcgccctggc gaccacggtc tgcgctcaaa actggccgca ggcgtccggg ccgaacctga
    86881 attggaaagc agacggagcc gatccgccgc tggaatggtc ggtcgttggc ggcaagaaca
    86941 tcctttggcg caccactttg ccggaaggcg ggcagagcgc ggtgacgatc tggggggatc
    87001 gcgcattcgt gacgacgcat cgcccgatgg atgaaacgag cgaccggctg gaacccaaca
    87061 tcgtcggcta ctgcctggat gcgaacacgg gctcgattct ttggaaagtc gatcttcccg
    87121 gcacggaccc ggttcaaacg gcagggattt tcagcgatgc gacggtgttt gcgccgatcg
    87181 ccgacggcga gcacgtctgg ttcttcaatc gctgcggttc gatgggctgc tttgatttcg
    87241 acggcaaacc ggtctggctc cgcgagttcc gcccgcgcac tcgtcacacg aaccgcgaat
    87301 gcgaaccgat cctcgtcggc gacaagatca tcaccgtcga agtgcgtaac aaagaagccg
    87361 cgcagaagct ggagcggcac aagccggtgc cggaaggcat cgaacctcgc gatgtctgga
    87421 cctacctgca cgcgattgac aagaagaccg gcaaagtcgt gtggatcgaa gaggcgggaa
    87481 cctgcgttca caacacgccg atggccgggc agttgccgga cggacgctgg gccatcgtgc
    87541 atggtcgtgg cggcggtcat gggcctttgg aagagcctta tggtgtgagt ttgaccctgc
    87601 tcgactccgg caagacactt tggagccgcg aagtcaaggg agacagttcc tacaattcgc
    87661 actggaatgc gaagcacgtc ttctggttcc aaggttccaa tcacctcgtc ctcgacacca
    87721 ccaccggcga gattctgcgg actcaagatg tcgcgaaaaa cgtgaacgtc accgatgcgc
    87781 agacgggcaa acgcgagtcg gggatcgcca tcaaaggcgg caagcgaatg ccgctgacga
    87841 atcagaccaa catcgtcgtc ggcgactggc actacttcct cgcgcacgac atcaccgcca
    87901 tcggccgtgt gaacatcgaa accggcagga ccgaatacct gcgcgtcccg gtgcaactcg
    87961 ttgctggacc ggaaccgagg cgaatctggg agaagaacga cgcgattccg aacgacacga
    88021 ggaactcacg cggcattgac atcgcactcg acaaacgcgc caagggcacg ggctggggcc
    88081 acgtctccgc cgcgagcccg atcctcgtca atcgccacct gtttttcccg atcatgaacg
    88141 gcacggtcta tgtcattgat gcgtttgcgg aaacgctgga tgaaaaggct ctcatcgcgg
    88201 tcaatgatct cgggccagcg ggcgagactt ggacgctggc ctcattcacc tatgcaaacg
    88261 gccggctgtg gatgcacacg atgaaagagg tcctctgcat cggtaagaac tgaccacccc
    88321 tgccagttta tcaaaaacgc acgaccccca tgatcatccg ggcccatact tctccaatca
    88381 gcaatcgcat ttcgctcgtc tcggtgttga tctgcgctgg ttttgtcctg cctgcgctgg
    88441 ctgccgagac atacacgccg ggcgaacccg ttcgcgcgaa gttcaaggac ttcgcgctgc
    88501 cattcctcga acagaactgc ttcgagtgtc acgacaacga catccagaag ggcgatctca
    88561 acctgctcga gctctcgcgg gtggacgaga ccaacgccgc cacctggaag tccgtttggg
    88621 ctcaggtcgc gctggaggaa atgccgccga agaagaggga ccagccggaa gtcattgacc
    88681 ggctgcgctt ctccgactgg atcgtgggcg aactgcaacg cgtgatgaag gacaagggcg
    88741 gcttccacgc ccatctcgat ccgaagaagg gcaacttcat cagtcacgac ctgctctttg
    88801 gcccgctgcc ggaaggcatc caactgacgc cgacttcctc acccgcccgc atctggcgtg
    88861 tgacgccgca agagcacatc acgcggctca atgaactgat caataccgaa cccaaatacg
    88921 atcccgcgaa gccgggcctc cgcgcccacg gcgatgtcgt tcccaccaat cacggcggcg
    88981 agctgaagct ctacttcggc atagacaaca tcaccagtgt cgtgggcggc acggtggcct
    89041 acgccacggc agtgaagagc gtgcccgtcg tgctctcttc ggctcgcaag gtcggcctga
    89101 agaactaccc cgatttctac acggtcaaca gcgccgaggc gacgcagatt ctcggcaagg
    89161 cggaggacat tctgaaatac atggcctacg ggccgctcag tctcgtcggc atgccggaac
    89221 agatcaccga cgatccgaaa acctacgaca aggtgaaacc gaaaggcgat ctgcgcggac
    89281 tgccgacagc cattgtttac aacaccaaag tcgttcgtcc gctgacgccg gtgctcgacc
    89341 tgatgaagga gccggaggtt accgacgaat gcctgcgtgc cgccgtggac tatctctttg
    89401 aagcgctgac tttccgccca ccaaacaaag cggagtccga cagctacttg cagatcgtga
    89461 aagacagcat cgccaaggtc ggcaaaaaag acggcgtcat gatgggcctg tcgtcgatct
    89521 ttctcgatcg cgacgcgttg ttccggccgg aactggtgga aggcggcaag ccagacgagc
    89581 acggccgcgt catgctgcag gattgggagc tcggtctcgc cgtgaatcac gcgctgcgct
    89641 acatcaagcc cgacgaaccg ttgcgtcagg cgatcctcga cgcccgcatg cggacacgcg
    89701 acgacgtgaa acgcgaggtt acccgcatgc tcgacgacga tagcatccgc aagccgcgcg
    89761 tcctgcagtt cttccgtgac tactttgatt acgaccaggc gggttacatc tgcaaagaca
    89821 cgcgcgccct ggcggcgacg ggcgtctcaa cacgcggcga atcccactac cgcgccatgt
    89881 ttgacgccac cgcaagcacg gaccgcttga tcgagctggt cctcgcggaa gacaagaacg
    89941 tgctcaagga actgctcacc acccagcgcg tcgtcgccac ccagacggac aacacctact
    90001 tcggtaggaa acacacgaaa gaagagcagg ctgccgccgt ggccgcgaaa aaaaaagccg
    90061 aggaggagtt ggcacacagt gagaatgacg agatcaaggc gctgaaggcg gaggtcgagg
    90121 cagcggaggg cattttgaaa agcacccctg atgacaaggc ggcgcagaag tctctcgacc
    90181 gccagaagaa gctactggcg gcagcggaga agcgaatcga aaacgccaga agagagcgga
    90241 agcgcggcgc cggtcccaac gccgaggtgg ccgaggccga cctgaccgga ccgccgatct
    90301 acgcccgcgt cagccgcccg accttcggtg ccggctccat gaagcccgag cgcattctcg
    90361 ccaccgctcc cgaaggccag cgcctcggca ttctcaccca tcccgcctag ctcgtttcgc
    90421 agtccgacgc aatggacaac cacgccatcc atcgcggcat ctggattcgt gagcgcctgc
    90481 tcggcggcgg catccccgac gtgccgatca cggttgacgc ccagctcccc gtcgagccgc
    90541 aaagcacgct gcgcgagcgc atgcgcgtga cgaagcagac gtactgctgg acctgccacg
    90601 aaaagatgga cccgctcggc ctgcccttcg agatgtacaa ccacgccggc atcttccgca
    90661 ccaccgagct cgacaagccc gtggacgcca ccggcgagat cattgactcc ggtgatcccg
    90721 ccctcgacgg acccgtcgcc aatgccctcg agatgatcca aaagctcgcc gaaagcgagc
    90781 gagtcgagca ggtcttcgtc cgccatgcct tccgcttctg gatgggccgc aacgaaaccc
    90841 tcaacgacgc ccccgtcctc caagccgccc accgcgccta ccgcgacaac ggcggcagca
    90901 tgaaggcgct cattaccgcg ctggtctttt cggatgcgtt cctctatcgc aaggtcgagc
    90961 gtgtggcgaa gacggcgggg aagtgacgcg tgcatgattc tgaacgcggg ggatgttcgg
    91021 gcgccttttt ctttgcaagt ccccggcagc ggctctaacc cgctcccatg aaactggaca
    91081 ccgccaccga atcgctcatc agccttgccc tggaggagga tgtgggcgcg ggcgatctca
    91141 ccgcgctgta ttttgtgccg gaggccgcgc gctccagcgc gcgtgtcgtc gctcgcgagc
    91201 cgggagtggc cgccggactc gccgtggcgg cgcgtgttta tgaaaagctc gatccgcgcg
    91261 tgagtgtgcg cgcgctgctg gcggatggcg acgcctttga aaaagggcgg tcgctcatgg
    91321 agatcgaggg tccgtcccgc tccgtgctca ccggcgagcg caccatgctt aacttcctcc
    91381 aacgcctctg cggcgtggcc gcgcaaacca gacgccacgt cgaggccgcg aaaccgcatc
    91441 ccgtgcaaat ctgggacacg cgcaaaacca cgcctggctg gcgtgtcctg gaaaaagcgg
    91501 cggtcaaagc cggcggcgga gtcaaccacc gcatgggtct ttatgaccac gtcatggtca
    91561 aggacaacca cctcgccgcg gaaggcggac tggaggctct gcaggccgcc atagaccgcg
    91621 tccgctcgga gcaccccggc gcccgcgtgc agatcgaggc cgacaccctc gaccaggtgg
    91681 ccgcctttct caccctgcgc ggcgtggaca tgctgctgct cgacaacatg agctgcgacg
    91741 atctgcgcgg ggccgtgcgc atgaacgcgg ggcgtctttg gcttgaggcc agcggcggcg
    91801 tcactctcgc caccctgccg caaatcgccg ccaccggtgt caacgccatc tccgtcggcg
    91861 ccctcaccca ctccgctcgg gcgctggatc tggggctgga ctttgatgcc ggcggcggtc
    91921 ttcgctgatg ccgccgcaca gcggtcgtgg accggtccgc gccttcaaga gccttgcatt
    91981 tcccgcgggg cgcgagtcaa ataccggccc catgatccgc caagccattt tcacggcatg
    92041 cgccaccctg ctggcctgtc cgccgattca agcgcaggaa aactccgccc ccgccctgcc
    92101 cgtgtcggcg cagacaaaga ccttcgaatt caaagacggc gaccgcctcg tcctcctcgg
    92161 caacacggtc atcgagcgcg agcagcgtta cgccactttc gaaccgcttc tggcgctcgc
    92221 gctcggagag agaaaagtca gcgtgcgcaa cctcgcctgg agcggcgaca ccgtcttcgg
    92281 ccacgcccgc tcctacttcg gtccgcccaa ggagggtctg gagcgtttgg aagtccacct
    92341 cgatctgttg aagcccaccg ttgtgctgct ctgctatggc tcggagatgg ccttcgagcg
    92401 gctttcgggt ctgcccgagt tcctcaccgg ctaccgaaat ttgctggaga tcatccgcaa
    92461 aaaatcgcct ggcgtgcgcc tcatcatcgc cgccccgccg cccctggaaa ccctgccgcc
    92521 accgctgccc gaccagaccg acgccaacaa ggccctctcc agcttccgcg acgcgctgcg
    92581 gaagttcgcc ggctcgcaga atgtcttctt cgtggactgg ttcgagttaa tgggcgggat
    92641 gccgaagccc ggagtggtgc aaagcccgct cacggaaaac ggcgtgcatt acacccgcca
    92701 cggctatgaa aaactcagcg ccaaactcat cgaggcgctt ggacttgaag tcccgaaaat
    92761 ctccagcgcc gaactcgagc cgatgcgccg ggaagtgctg cgcaaggacg agctcttctt
    92821 caaccgctgg cgtccgcaaa acgagactta tctctttggc ttccgcaagc atgagcaagg
    92881 ccagaacgcc aaagagatcc ccatgttcga cccgctcatc ctcgaagcgg acggcaaaat
    92941 ccaggaaatc aaaaccggca ttctcgacgc caggaagcgc ctttaatcat caagccgacg
    93001 cacaaagatc atgattgccc gcatcctcct tctcagcctc gcttgctgcc tcccgctcgg
    93061 agcctcccag ccaaagtcca ccgccgacct gcccgacccg aatcccgagg tgcaaaaggc
    93121 cggctttgtc gtgcccgaag gtctcgaagt caccctctgg gcgcaggagc cgatgatcca
    93181 aaagcccgtg cagatgaact gggacgcgca gggccgcctc tgggtcgtca gcagcaccac
    93241 ctatccgcag atcaagcccg gtgatggcac caaggaccag gtcgtcgtgc tggaggacac
    93301 cgatggcgat ggcaaggccg acaagtccac cgtcttcgcc gaaaacctgc acatccccac
    93361 tgccgtcatt cccggtgatg gcggcgttta tgtggcgaac tccaccgagg tgctctttct
    93421 caaggacacc aatggcgacc tcaaggaaga cgagcgcacc gtcatcctca gcggcttcgg
    93481 caccgaggac acgcaccacc tcctgcacac catgcgccac ggaccggagg ggctgcttta
    93541 cttcctccag tccatctaca tccacagcca catcgagacg ccccacggcg tgcgccggct
    93601 catgggcggc ggcgtctggg agctccgctc ggagacgcgc cggctggaga ttttgagcaa
    93661 gggactcatc aacccctggg gcttcgagtt cgacaaatgg ggccagagct tcgccaccga
    93721 tggcgcgggc ggcgaggggc tgaactacat cttccccggc agcgtcttca agtcctcccc
    93781 cggagccgcg cgcaccctca acggcctcag ccccggccag ccgaagcaat gcggcctcga
    93841 atatcttgac gatccccact ggcctgagga atggcgcggc acctgggtca ccaacgactt
    93901 tcgcggcaat cgtgtgaacc gcttcaagct cacgcaaagc ggcagcggct acatcgccaa
    93961 acaggagccc gatgtcctcg cctccaacca ccgcgccttc cgccccattg acgtgcgcac
    94021 cggcccggac ggcgcgctgt acatcgccga ctggtacaac cccatcatcc agcatggcga
    94081 ggtggacttt cgcgatcccc ggcgggacct cgtgcatggc cgcatctggc gcgtcaccgc
    94141 caaaggccgc ccgctgagcg ggatgccgaa gatctccggc gcgcccgtgc ccgagctgct
    94201 ggaaatgctc aagagcgagc gcgcctggac gcgccacttc gccaagcgcg agctgcgcga
    94261 gcgcggtgtc gccgaggtta tgcccgtgtt ccgcccctgg gccgacgcct tgccgaagga
    94321 gaccggggcc gactggcacc tcatgctgga ggccgcctgg acgcgcgagg ccctgaaccg
    94381 tttttccgcc aagcactggc gcgagcttta ctccagcccg gacgcgcgca tccgcgccgc
    94441 cgccctgcgc atcctcaccc accgctggcg cgagctgccc gaggcgctcg acatgctgaa
    94501 aaaagccgtg gccgatgaaa acgcccaagt ccgcctctgg gccctagccg tgctctccga
    94561 aatgcgccag ccccaggcat tcgatgcggc cgtgcgcgcg ctcgacatgc ccatggacga
    94621 gaacctcgac ttcctcctcg aactcaccgc ccgtgagcag gcatccatct ggttgcccgc
    94681 ttatcaaaag gaggtcatca agctcaacga aaacccgcgc cacctcgtct atgccatgcg
    94741 cgcctccggc agcagcgaag cattgaagcc tcttttcgat gccctcaaag aaggccgcct
    94801 ccctgccgag gacgccgccg ccgcgctcgc cctcgccgga gatgtggcgg atgccgccca
    94861 agccgcgcac atgatcaccc tcgcgcaaga ccccgctctc gccgcgcacc tcagcggcat
    94921 cctcgacgcc ctcgtcaaag ccggcaccac gcgcggcatc atcccgaaag acgccgaggc
    94981 cgccgtcacc gcctggctgg ccgacccgcg cgccgaagtc gtccaccgcg ccaccctcct
    95041 cgccggagcc tggaaggtgg aaaacgcccg cgccactctt gagggcatcc tcaccgccga
    95101 gaccaccgtc ccgcccgtgc gggaaggcgc catgcagggc ctctcacggc tcggcggcgt
    95161 caaagcgcgc gacttttttg accaggtctt ccaaagccac cctgcccacc gcagccttgc
    95221 cgtgcagggc ctcgccgcca ccgggccgca gctcgccgcc aagcgcgccg tcgagttcct
    95281 cgcctccgca aagaccatgg ccgaggccgc ccccatcctc accaccttcc tgaacagcaa
    95341 gcaactgccc gccatcctcg ccaaggaact cgccggcaaa actattccag aacccatcgc
    95401 actcgaagcc gtgcgcatgg tctccacccg cggcattcag ggtccgctcg aggacgcgct
    95461 gcgcgccgcc ggcggtgtca aatccatgag ccaaagcctc acccccgagc aaatggccgc
    95521 catggtcgag aaggtcaaaa cccatggcga tgccaaacgc ggcgaggaca tttaccgccg
    95581 cgccaacctc ctctgccaga cctgccacgc cattggcgac gccggcggcg tgctcgggcc
    95641 gaacctcgtc agcatcggcg gcagcgcccc cgtggattac cttatcgaga gcctgctcga
    95701 cccaagcgcc aagatcaaag agggctacca catgatcatc ctcaccacca aggacggcgg
    95761 tgtcatcagc ggcggcctcg tccaggatgg caatgaggaa gtcatcatcc gtgatcccgc
    95821 caaccagatt caaaaaatcg ccaagtccca gatcgccagc cgccagatga gtcccgccag
    95881 catgatgcct cccggcctca ccgccagcct gcgcgaggat gagtttgtgg atctcgtccg
    95941 cttcatgtcc gaactggggc gcgagggcga gtacaaaatc aagcccaacc gcttcgtccg
    96001 cgcctggcgc gtcatgggcg ccatgacggg cgagcagatt gaccatgtcc gccatgtcgg
    96061 cctgcacgcc ctgcatgaaa aggacggcgg ctacccgtgg gaggtgcgtt tctccaaagt
    96121 cagcggcgcg ctgcctctga acgaggttca gatggcgaag atgtacccat ggttccccaa
    96181 gatcgcccaa atcagcctca agctcgacag ccccggcaag gtgaagctcg gcctcagcag
    96241 caccaaggcc gtggatgtcg tcgtcggaga ggccgcgctc aaagaaatca ctcccgaact
    96301 tctgctcgac ctgccagccg gcacccaccc cgtcacgttc gtcatcgggc gtgacgcggg
    96361 cgaactcgcc agcttcagcg tcgagatcct cgaaggcgcc gcccaggtgg cctactgagc
    96421 cgcggccatg ccgctcgcct ccaggttttt ttgagccatg attgtgtcgt agacacagaa
    96481 atccgccatc cttgcctgcg aggccttccg gctgtgacct cgctcctgaa actgagaaaa
    96541 cactggagaa aaaaaagccc ttggctggta ggatgcttgg ttccttccac aagtcataat
    96601 actgccatct ttcgcaccca gtctcatcct ttcgatcctc gcatcctgct cgactcaccc
    96661 gccggaaaag cgccgcaagt gtccgggcag gaaaggaatt gggttctgga tttcaccaag
    96721 cgctacaatc tggaataccg cgatggaaat gagacgcgca gtctgccggg ctccgggatc
    96781 agctatctgg atgagagccg gaggtgagcc gccggaaggc tggaatgccg gttttgcatc
    96841 ccaccagctt attcaacaga taacctccct gttaccacat tctcgaatcg ttcacagaaa
    96901 cgaacggcag gaaacgcctg ctttttggcg tgacggtctc gccatcaaat ctggcgctct
    96961 tgtttttcgg cggcatcatc tggttctggg aatgggcggt cactgccgtg ctgctgcttt
    97021 ctgctgggag gtccgaaacg catcgcgctg gtggaggccg atctggcgcg cattggactc
    97081 cttcgcccgc ctgctcaatc gggctgcgct caacgctccg cgtagtcttt cacactgagc
    97141 ctgcttgctg agttcggttc tagaagcgcg cggcggtgtc ttgaaaaagc tgttcagcgc
    97201 ggcaaagctg tgcttcttcg actccgacca tggccacacc ctaccacatc gttcccaccg
    97261 ccgcccataa tgacctcgtc cgcgccgcgt atctgcaccg cggctacact gccgcggagg
    97321 cggaggatgc ggcgcgcttc tgcgagatgg cgtcggcgca cggcatccgc acgcacaacg
    97381 cgatcaaggc gctgcatttg gatcatctgt tcggcagcgc caccgggggc tgcaagccgg
    97441 gggcggagat ccgggtggtg gagaagaaat tcgcggcgag cgaggtgtgg gacgcgcggt
    97501 tgaagctggg ccagagcgtg gctttccggg cgatggagcg ctgcatggag ctggcggacc
    97561 agtacgggat cggccaggtg agcgtggaca acgcgtttca ttacctgtgg ggcgggggtt
    97621 atgtgatgga cgcggcgaag aaggggtata tcgcctacac gaactgcacc tcgacgctgg
    97681 cggaggtggt gccatttggc ggcaagtttc cggtgctggg gacgaacccg cattcgtggg
    97741 gcctgccgac gcaggatgcc tgcggctttc cagtggtgat tgactgggcg acgagcacgg
    97801 tggcgatggg gcgggtgcag caactgaagc gcgagggcaa gccgctgcct ccaggagcgg
    97861 cggtggacgc ggaggggaac ccgacgacgg acgcgaacgc ggcggcgtgg ctgctgccct
    97921 tcggcgcgca caagggctac ggcctctccc tgctcatcga gctgatgggg gggatgatcg
    97981 gcggcagcct gcccacgctg cgcggcggcg gcgcgcaccc cgaggtgaag gcggcgggtt
    98041 ttcccgccgg ggaaaagcgc acgagcagtt tttatttcca ggtgatccac ccggacgcca
    98101 tcagcagcgg catgtttgcc cgggggcgcg gctttgcgga gaatctgaag gcggtgctcg
    98161 atgacatcct gggccacggc aacgagggct gcatgctgcc cggccagcct gaggcgcaaa
    98221 acgcggcgca cacggcgaag gcgggcgggc tgcttttctc cgccgccgag gtggaggcgc
    98281 tcaatgagat cgccgccgag tgcgggagcg cggcctttga agcgtccgcc ttgccggttt
    98341 acgaagtgtg aggcatggaa tgtctccctt gaagctgaat ccaatggccg cgcccgcccc
    98401 cgccaaagac atcaagatcg ttccgcgcca tgtggccatc atcatggatg gcaacgggcg
    98461 ctgggcgaag gagcgcggac tgccgcgcac ggaagggcac cgccaggggg ctgagacggt
    98521 gcggcgggtg acggaggcgt gcggggacat cggggtggaa tacctgacgc tttacgcctt
    98581 ttcctcggag aactggaagc ggcccaaacg ggaggtggac gcgctgatgc ggcttctgga
    98641 gcagtttctg cgcaaaaaga cgcccgagat gagggagcaa aacatccggc tgcaggccat
    98701 cgggcggctg catgatctgc cgaagccgtg ccaggagcag cttcaccgct ccatcgaggc
    98761 cacgtctcag aacagcggcc taaccttgat cctcgcgctg agctacggcg ggcgggagga
    98821 gatactcgac ggcgtgaaaa gcctcatcga gagcatcgag cgcggccatc tcgacaaggg
    98881 gatggtggac acggatgtgt tcagcaagca cctgtacacc cgctactatc cggaccccga
    98941 tctgctcatc cgcaccagcg gggaactgag gctctcgaat ttcctgctct ggcaggtgag
    99001 ctacgcggag ttttacatca cgcccaagct gtggcctgac tttgacaagg aggatttttt
    99061 cgaggctgtg cgcgaatacg ggaggcggca gaggcgcttc ggtggcgtgt aggtcagacg
    99121 tgccggcttg tcacgagcgc aagggccatc ccgcctgttt ttccgccagg atgctggagc
    99181 gcgcggtatc aaacggatga gggcaaccgg gacggctgcc ccggcatctc acctggccac
    99241 atagtccacc acgcagacct tgtcgagcag gggcttgagc ttgccgacga actcctcgtg
    99301 cgcggggtgc gggagataca cttcgagtcc cgccttgttt gcgaaggtga ccagaaagca
    99361 atgcgtgaag ccatcgttga ggccctcggg gctgacattg gtgccccatt cgaaggcctc
    99421 caccgtttcg atcttcttcg ccagttcggc gaagtcgcgc tcgatgccgg ccacttgctc
    99481 cggggtggcg ctgtccttga atttaaaaaa caccacatgg cggtaggggg tgttggcgag
    99541 ggcggcggtg gcggtggcgg tcatgaggag ggtgaggagg acgaggagtt tcatggcgct
    99601 ggatggaaga cgcgatgcgg ggggcggaca atgggaaagt ggaaaccgat tatcgaatat
    99661 cggatttcga tttttcaaca ctcaccccag ggtgaggcac accggtagtc cctcccggat
    99721 gtccgcccac ggtgcctggt ccgagacatc ctggggatcg ggcacggcgg cgaggtcgtc
    99781 cggcagcttc gcgggcgaga ggatggcgag gaagacgagc ggctcgtcat gcgggttgta
    99841 ggtggcgtgg acgacacctg gagggatgtg agccatctcg ccggcgcgca ggatgcgttt
    99901 ttcagagcca acccactgct ccgcgcggcc atagaccaca tagatgatct cctcccggtg
    99961 gggatggaaa tgaaaggggt ggcagtggcc ggggtccatg ttcgcgcgca cgaggagcag
   100021 cttctccgcc tccaccaggt ccgggcggca aagccattcg ttatgtgtcc aggggttggt
   100081 ctcgcggaca gcgttctcga tctggacaaa gcgggcggat gtgtcgggca tggcggcatt
   100141 ttggcgggct gatttgaatt tggcaaacgc ctgttgccag cctgcgccca cctgcggcta
   100201 ttcgttccac actcgatctt ttgcattcgc gcgcattccg cgtagttgag gcatgaaaat
   100261 actcgcagcc atgtcgggag gagtggacag cagcgtcgcg gcggcgctgc tcgcgcgcga
   100321 ggggcatgat gtcacgggcg cctacatgaa gaactggatc aacgaggaga atgtcgccgg
   100381 ccattgcccg tggcagcagg acattgagga cgcgcgggcg gtggcggagc ggatcggcat
   100441 cgggttccag gtggtgaact tgatgaggga gtaccggcag cgcgtggtga aatacctgct
   100501 ggagggctac caggagggtg tgactccgaa cccggatgtg atgtgcaacc gcgagatgaa
   100561 gtttggggtg ctctgggact gggcgcggga gcggggcttc gatgccatag ccaccggtca
   100621 ttacgcgcgc aaaacgcccg atggtcctgg catcctgcgc ggtgcggacc cgaacaagga
   100681 ccagtcgtat tttctggcca tgatgcgtcc ggagcaggcg cgcatcgccc gattccccat
   100741 cggtcatctt ttgaagccgg aactgcgcgc gctggcgcgt gagatgggcc ttcagactgc
   100801 ggagaagaag gacagccagg gcatctgttt catcggggag gtgcggatgg aggactttct
   100861 gcgcgccttt gtgcccgacc agcccggccc gatcgtggat ttgcaggggc gggtgctcgg
   100921 cgggcacaag gggttgcatt tatacacgct cggccagcgc aagggcatcg gcgtggccag
   100981 cccggttcac aaacaggcgt atgtggtggt ggcgaagcgg cctgcggcga atgagctggt
   101041 catcgcgatt gaaaacgagg acacgccgct gctttgggcg cgccgctgcc gggtgtcggg
   101101 cctgagccac gtcaccgagc cattggagac tccgcgcctt ttccaggcgc agccgcgtta
   101161 ccggtgcccg gcgggcgcgg cggatttcac cccgctcggc ggcgggcgcg cggagatcgt
   101221 cttccaccag ccgcagcgcg cgctgactcc gggacaaatc tgcgcgcttt atgacgggga
   101281 gcgtttgctg ggcggcgcgg tgtttgagag cgtggaacac ggggagtgac gcgccggtct
   101341 ctcggctcac ggattcatgc cgtccgccgc gccccagggt tcgagcaggc ggaggctgcg
   101401 gtcgcgcagg gcggcgtggc agtcgggcag gtcgaaatgc gccaggatgt tcggcgggag
   101461 catggagtag ggccatttgt agtcctcggt gcgtgccact tcgaggtagg aggagaggcc
   101521 gtttttgagc gtcacctgcc gggcgcgcgg ttccaggacg gcggcaaaga cggcggccag
   101581 agcgccccag ccgcggccgg cgaggtggat ttcctcatgc ccctggccgg cgagccattc
   101641 gatgacgcgc agagcgtcaa agacccgctg gccgaggtag gggcggtcga gcatgaggga
   101701 gtgggcgctg atgaagtagt cgctgccgta ggggcggagc atttgattga tgccgcagat
   101761 gtcgggctgg ctgtcgccga tgccgcgcag gtcgcaggca tagaaggcgg cgcctttttc
   101821 ctccgcgaga agctgctgga tgagaggctc gccgcgcagt tcctcatcgg cggagcggtg
   101881 ggagaggtaa agcacggcgc gcttctggcc gagaggcggg cgggaggtga gggtgtcatc
   101941 atgcaggcgg gtgacgagcg cgtggatgcc cggctcggtc tccacggcgt aggcgcagta
   102001 gcctttggcg ggatactggc gcgcccccgt ggcgcgcagg atgcggtaat cgggcgcgct
   102061 gccaggagct tcgggaattt tcaacacgga gcggatggcg tcctgaaggg ccacaccctc
   102121 cacgggaccg cgctcctgag cgagccgggc ggctttttcc tcggtgaagg agaaaagggt
   102181 gcgcgaggct ttgcccgcct ggccttcggg gaagcaccag agggtttcat ccttttcgta
   102241 ggtgagtccg ggttcggcct gcgcgtcgga gacgccggtg gcgagattga aaaagcggta
   102301 catgccctcg cggttgacct gggagtagcc gtggtagtcg ccgccgatgt gcaggcgtgt
   102361 gttgtcgggc tggccgagca gggtgtagag ggttttgagg cgctggtggg cctcggtggc
   102421 gccgcgcgcg tcgaagtaat ctttctcctg cgccaggatg agggtcggct ttggcgcctg
   102481 ggcggcgaga aagtcgctgt ggtcgagatc gagggccagc acgcgcggcg ggcattgttc
   102541 ggtgtcggct ggcagttcgt tctcggcatt gcggcggaag gtggtcacga agcaggcggg
   102601 tgcggccatc gtccagcgcg gctccacgcc gcagagccag gtggtctggg tgccgccgcc
   102661 ggaattgcca gtgacgccga catgggcggg gtcaacctca ggccgggtga gcaggtagtc
   102721 gagcgcgcgg atgccgtccc aggcgaacca ggagccgagg aactcgccca cgagggtctg
   102781 ctggttgccg gcctgaatgt gctcgccggt gccgggattg aagcgggact tgaagccgtt
   102841 gaggtattga aagcgctcgc cctggccggg cgggtctatg atgaggcaga cgtaacccat
   102901 gcgcgccagg ccctgagaga acgcctggta agcctccgcc gccttgccgt tggtggagtg
   102961 tccgcacacg ccgatgacgc ccggcatttt gactgggcgg cctttgggca catagaggtt
   103021 cccggtgacg agaaagccgg ggcggctctc gaagacgacc atttctatgt tgtaggcgtc
   103081 gcgctccacc acgcgggtga cgcgcgcgtt gagcggcgtg cgctccggct ccgggccgaa
   103141 gcactcgcgg atgcgctccc gcacggactg gacgtaagcc tcggcgtccc cgcgggtctt
   103201 gagcgccgcg cggcgggcat tgccgcgctc ctcggcggcg cgcacctggc cgaccatcca
   103261 ctcctgcacc atgtgcggga aacggttcag cggcggcagg ggcgcggggt cggatttcgg
   103321 cttctgagcc aggctgtcgg tggcgagcac gaggcagaag gcgactggga atatatgggg
   103381 cagaatcatg ggttggaagg gatgacttgt cagcataccc cgatgagtgc ggagttcttt
   103441 cccactcatc cgccgcctcg gagaggggag catttgcgtt tcgggagtga ggcggcaggg
   103501 aagtcttgtt tttttaaata cagagtatgg attgaatata atttatcata attatttgat
   103561 tttctaaaca aagtggcgaa aatgcggcga atatttttga aaatatgaaa aatgcatccc
   103621 ctctcccgaa tgctggcacc catttttctg gctttgccct ggtcagtttt ggtgaactca
   103681 acgtgccggg cggccccatg gagttcggtt ttcaactcga tttcgtaccc gatgaaattc
   103741 aagcgcggca actgattttt ggactcctat gccactcgga gaactcgccc gatggcgagc
   103801 acgggttcgc ggtgcgggtg gatttgacga ccggggaaat ttgggacgcc cttaacgaca
   103861 acggcattgt cggctgggtg gaaaacccgg acaaactgga gcgtttcagc accgaggagc
   103921 cgctgctgat gagctggaga gtcgagcatc tcggcggagc cttgattccc aagctgcaca
   103981 tcgccgggga agatcatctg tatccctcgc tgcggtacga accgggcatg gtcatgacca
   104041 ccgtggccgg tacggatggt ggtttgcaga gcgctttaaa cggcttcatg caccccgccg
   104101 tctggcagga gttgatgtga gtggcgggtg agtccgcagc ccggatggac tgcggtcatg
   104161 cgacgatggc cgtcattttc acggctttgc cgccttcggg cggttgagca cgtcagcacg
   104221 ggtgctttcg gcgatggcca gcacttggtc tatgagggcc ttgtccactt tcaggtcttc
   104281 cagggtggat ttcagatggc cggcgatggc gttgaagtgc tcgtcgttga gaccgggaag
   104341 gtctctgtgc gctgcgcgca tgtccttgcc ggtccagggt gccgggcctc caaaggctgc
   104401 ggagagaaat tccttctgct tgcggtgctg cttcaccatg ctcacgtcct cgaagaagtg
   104461 gttcacccgc ttgtcggcga gcactttcac atagaaggcg tccacggcgg cgtctatcgc
   104521 ggccttgccg ccgagcttgt gatagaggct gctttcgcgc gcttcgatga attttttccg
   104581 gcatgcctcg acggcgaagg cataggtcac gccttcgtat tcccaggtga tggacgggtc
   104641 cacgggcttg ccgctgatgg gacagacggt gttggcggca tttccgggtt cggcagcggt
   104701 gagcggaaga caaagagcga gggcgagaag gacgatgatt tttttcatgg cgggtggagt
   104761 gtgaattgag gctgaaccct gaatgggtaa ataaaatacc tgttttgcaa gcgagtcttt
   104821 cagtgcaatc aaaaaaacaa cccctcatct cccgccgccg tgcaactgac ctaccacacc
   104881 gactacgcgc tccgcgtgct catctacctc attagcagac cggagcggaa ggtgacgacg
   104941 cgcgagatgg ccgaattcta cggtgtgtcg ctcaaccacc tgaccaaggt ggccaaggcg
   105001 ctcacccgcg cgggctggct ggtctccacc cggggcgctg gcggcggcct gatgctggcg
   105061 ccgcacactc cggacacaag gctgggggag atcgtccggt tcactgaaag cggtgacatc
   105121 gtcgagtgct tcaccccggc cacaaacacc tgcccgatca tcaaatcctg cgccttgaag
   105181 cccgtgcttt accaggcccg ccgcgctttt tttgaggttc ttgacgcgca tactgtgcgg
   105241 gacctggccc gtctggaaaa gtagcttttg catgcaacta aacccgcgcc cggtgaatga
   105301 caaccggtgc gtggcgaggc ggtggtccgg gtgcttttgt ccgctcatgg acaaccatcc
   105361 tcactcccgc agtcggtggt tgcgcggcat cagccttggt gcatgcgcgg ccgtgtctct
   105421 cgctaccttc aacctccagg ccggtgctcc cgcgcccaaa aatcctgtcg tgcccgtcga
   105481 ggctgtggat tggaaggcgc gcacgatctc cccggtgaca aaccccatct tctttgagga
   105541 cgccgtcatc cgctcggaga tccgccccat tttcgcctac cacaacattc acgacgactt
   105601 tcttggcggc ggggacgccc aactctacgc gctgcaaatc cgctacgccc tgaccgaccg
   105661 cctggccttc atcgccacac aggacggcta ctttgacatc aacaacgacg ccatcgccaa
   105721 tcccgaaggc tggatggaca tcgcccttgg cttcaaatac gcgctcattg atgacatcga
   105781 aagcgagttc atcctcaccc ccggtttcac cttccacatt cccacgggag agcgcgatgt
   105841 cttccagggc cgcggaggcg gcgagtggaa tctttttgtc tccacccaga aaggctatgg
   105901 cgacctgcat ctgagcggca acctcggcgt gcgcctgccc aacaacggca gcgaacagag
   105961 caccatgctg cattactccg cgatggtgga ctatcacacc tgccgctggt tcattccgtt
   106021 catcaccttc aacgccttca cggtccttga tgagggcggc aacatcccgc tcaacactga
   106081 gggttacgat gtgatcaact tcggctcgtc gaacactcgc aaccgcacgc agggagcgct
   106141 cggcgccggg ttccgctccc gcattcttga caatgtggac ctcggctttg cctatgagaa
   106201 ggccgtcatc aagcccgagg gcctgttcga cgaccgcttc accgtggaca tctgcatccg
   106261 gttttaacgg cctgaaaggc cgtgggcgtc aggtgtgacg cctgtttggt cgagtggtga
   106321 ccgggcgcat ggaggcagct ccatgcgccc tttgcttttg gcgcgcgcgc cgctatggat
   106381 gcggactcat gagatggtcc ttcaaaatcg ccaccgtcgc cggaaccgag gtgcgcattc
   106441 acatcacctt tttcatcctg cttgcctttg tggcccttca cggcatgggg cgcggggagg
   106501 ggctgccggg agcgctggac gcggtgcttt tcatcagcgc catgtttttt tgcgtgctgc
   106561 tgcacgagtt cggccatgtc ttcgccgcgc gcggttacgg catccgcaca ccggacatca
   106621 ccctgctgcc catcggcggt gtggcccgcc tggagcgcat gccgcgtcgt cccgcgcatg
   106681 agcttgtcgt cgctgtctgc gggccgctgg tcaatgtcgg catcgccttg ctcatttatc
   106741 tggcgctggg tttgagactg gatgccgacc ctgccgcctt ccgttttgag cagtccggtc
   106801 atttttggga aaagctcatg ctgtggaatc tgctgatggt cgtcttcaac atgatcccgg
   106861 cttttcccat ggacgggggc cgtgtgctgc gggcgctgat ggcgatggtt tttgattacg
   106921 gcaaagccac ccgctgggcc gccgccatcg gccaggggat cgcgctgacg gtggtgctct
   106981 ggatgctgct ggcaggcgtc ttccagccca tcctgctgct catcgccttc ttcatcttca
   107041 tggccgccgg ccaggaggcc gccgccgtct ccgagagcga ggccacgcgc ggcctgcgcg
   107101 tgcgggatgc catgctcacc gacttccaca ccctgcctcc ggacgccgtg ctggcggatg
   107161 cggtggacct gctcatctcc ggctcgcagc acgactttcc cctgctggag cgggatggca
   107221 gcctgctcgg cgtgctcagc cgccagcggc tcatcgaggg cctttccaag cacggcacgc
   107281 tgcaccttgc ggcggattgc gcggatgaat gcgcggagtc agtcacaccc gtcaccagcc
   107341 taggcgaggc ggtggagcgg ctgcgctcca tccgctgccc catgctgccc gtgctggatc
   107401 cggtttcggg ccgcctcgtc ggtttgctca ccgctgaaaa tgtggccgag gcgctcatga
   107461 tccgagccgc cctgcggcgt tgatgctggc agggcgggcg ctcgcggttc agactccgcg
   107521 tcgctggcgc ggcgcggtct ggcgctcgtg accagccgcc ccgctcttct tttgaaccgt
   107581 ccacttcacg cttctgtctc ccttcctgcc ttgcgctttg cggcccgtcc ggtatcatcg
   107641 ttttccccaa accgccctcc atctcagcct cattcatgaa aaccgccgat gaactcatgc
   107701 tcgtgccccg caaggagggc tttcaggcat ggcgttcgcg caacgggtcc ggcacccatc
   107761 ccgagcctga gaaccgctcg cgcaagggcg cggactggat cgccctgccg gctcgctgtc
   107821 ttgtctcggt gcccatgcac ttccagggtg tggatgccgc ccgccgcgag gcggccgcgc
   107881 agctcgaact ggaggccgcc ggtttcgcag gtgagacggc ggaggcgcat gacttcgaat
   107941 tgtttcctct aggcaatgac tcccgtgatc ttcccgcagc ggcttttgtg caggtggccc
   108001 cgctgcccga gactgtgctg gatgaggggc gggacgcccg ctttgccccg tcggtggctt
   108061 ttcacaagct caatcccggc gagctgctga tctggcggga ggacggccag ctcgtgctgg
   108121 ccgtgccgca tgagtccggg cagccccttc actgccaggc gctcgccgcg cggcatctgg
   108181 acgacgatgc cgctgcggaa atacgctgcg tgctcgcctc gctcgatctc gccgcgctca
   108241 tgccgccggt gcagagtgtg gctgtcgtcc tgtccgatgg cgagaccgac gagtcactgg
   108301 cggccttcga ggcggagttg gaccttccgg ttgtggcgcg cagagagacc gctcccgtgc
   108361 tgcctgggca aacctcgcgc cttattccaa gccagatcgt gagggaacgc gcggagcgtc
   108421 agcatcggcg cctcgtcgcc atgggcgccc tggcatttgt ttttgtgctt gtcgccgctc
   108481 tcggagcctt tgccgtgcgc gtgggcctgc gcgaccgcgc gcttgccgcc gagactgccc
   108541 gtcttgatgg attggagccg gagctggccg ccatccgcga cgcgcgcgag gcctgggagg
   108601 acatgcgcac cgccgtcaac cccgagcatt atcccgtgga ggcgctttac cagctcgtgt
   108661 ccctgctgcc gccggagggc atccgcctga cccgcttcga ggtgagggaa gacggcatgg
   108721 ttctcgacgg cgaggcctca agcctggggc atggcatcga gtttcgtgac aaacttgtgg
   108781 gagcagatgc cttcaagcac tggcgctggg actttcccca gcccaccaac ctgcccgacg
   108841 ggcgcgccac cttccgcgcc gaggcgcgcg tgcctgatga gagtgaaacc gacggaggcg
   108901 aatcgtcatg actgccagcg aacaacgcct cgccctcgga ctcggcgccg tgctcgtcat
   108961 cggcggcgcg ttcatcggac tgacccggct gaaaagctgg aagcagcgag tggacgctcg
   109021 cgccatcgag gtgcagtcgc gcaaacttga ggctgacgat ctgctgtccc agcaggattt
   109081 ttggaacaca aggctgcaat ggctggagca aaagcagccg ctttacacgc gccgcagcga
   109141 ggccgacctc agcctgctgg atttgatcca aaaatccgcc ggctcgcatg gcatcaccct
   109201 cacccagaac cagcccgtgg cccccagcga gcgtcccggg ctgacctcct cgaatatgaa
   109261 tgtcgaagcg cgcgcgggat gggaggccat gaaccgctgg ctgcatgatc tgcagcggcc
   109321 tgagtccttc atcagcatcc ctgctctgac catgactcca aatgaggagg acacttcgga
   109381 ggtcatcgtg aacatgaaca tccagaaatg gttccgcctg ccgccgtcat gaagacctcc
   109441 gccattctgt ttctattttg ggctgccgca accgcggtgg cgcaggacca gcctgcggcg
   109501 gatgactcgc ccgctgtggc gatgccgcag gccggcgttt caggggcgcc tcccgtccct
   109561 gtcgaaatac cagcggaagt ggattcgccg ccagtcgaaa tgtcagccct cgcgcgcgag
   109621 gggcagccgg ccactcccgg cgctgatgat ccgccggagg agatcgccgg tgaaaacatc
   109681 cccaaggccc accctgactc ccgctaccag gctgcgtggt caaagaatcc cttcatgctc
   109741 aaaacggtgg ctgtggcaca ggccaccgtc tcctttgccc aggactgggc gctggcaggc
   109801 atgtacaaaa gcacgacggg caagatcacc gtcactcttc agaacaagca gacgcaggaa
   109861 taccagcgcg tgaccaacga ggacaccgag ggagagttcc gtctggtcga agccaagttc
   109921 aaccgcaacc gcaacgaggc gtctgtcatc atagccaagg gcagccagtc tgcggaaatc
   109981 aaatatgacg actccctgat ctccaagccc gtcaccatca acaacacgct tcgcccgccc
   110041 gcgaacgcgg ccggccagcc gggcgcgcag cagccaggga tgccgggcgc tccaggtgtg
   110101 cctgtcagcg ccactggagg agtgggcggc gttccccgcc ccggcgtcac cgtgccaggc
   110161 acaacgcgca ctctagttcc cggagccgtt ggcgcgccgg gagccgcgtc tgctgtcact
   110221 ccgccctcca tttcacgccg gcgtcaattg atccctcctc ccaccgtcgc cccgcctccg
   110281 caaccctgaa aagcagccca tgaaatactt ttccagaacc tcccaaatca tcagccttgt
   110341 cgggatgatc tgtctttttt ccagttactc aggaggatca acttgttcgg cgctccccga
   110401 agcgattccg tcggcgcgct ttcaaaatgt ctggtgcgcg gaaaaaaatc catttctccg
   110461 ccaagccccg ccgcccggtc aggtgcccgc agaatcctgg gcgcgtgact ggtcattggc
   110521 aggcatctac aaaagcacgg acggagtggt cagtgtcttt ttgaaaagca aaacgacagg
   110581 catgcacagg caaattcaaa ataacgacgc caatgaccgc ttccagatcg tcgaggcgcg
   110641 gcttcaccgc aaccgtgact cggcgcaagt gaccttgagc ctggatggtg tcactgcgga
   110701 ctttggattt gaattcaccc ccgccatgcc cgcaccgccg tcagcggttg caccgccctc
   110761 catttcaaag cgccgcgcat tgatcacgcc tccgcagccc tgagcctgaa cctccatgaa
   110821 ccgcagccat ctttttctcc ttcttctgtt cggtctttgc cctgcattcg cgcaggccca
   110881 gataccgccg caggggcagc gcccggccag tccgaacgcc cagcgtccgc agttgcccac
   110941 tcagc
//
LOCUS       DAOMYJ010000002        64104 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Prosthecobacter sp. isolate AMR_MDS_5629
            NODE_183_length_64104_cov_21.416291, whole genome shotgun sequence.
ACCESSION   DAOMYJ010000002 DAOMYJ010000000
VERSION     DAOMYJ010000002.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867850
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prosthecobacter sp. (wastewater metagenome)
  ORGANISM  Prosthecobacter sp.
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Verrucomicrobiaceae; Prosthecobacter.
REFERENCE   1  (bases 1 to 64104)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 64104)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.30
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:34:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,742
            CDSs (total)                      :: 4,684
            Genes (coding)                    :: 4,676
            CDSs (with protein)               :: 4,676
            Genes (RNA)                       :: 58
            tRNAs                             :: 55
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..64104
                     /organism="Prosthecobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_183_length_64104_cov_21.416291"
                     /isolate="AMR_MDS_5629"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1965333"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            275..1147
                     /locus_tag="PLP58_00505"
     CDS             275..1147
                     /locus_tag="PLP58_00505"
                     /inference="COORDINATES: protein motif:HMM:NF015867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspK"
                     /protein_id="HRJ07030.1"
                     /translation="MVWALLLMSVTVMGVVEYIRYSAEEAKLGAAEFYALHLAESGLA
                     VGLHPEARRGDVVLKQKIGADSGFDVVINYEGARIPINYITDDRLREAVYQLLIRWGL
                     NSDEAGVASESLADWVDRDDDVRANGAESAFYQQQGINDMPRQAGFIDVDEMLLVRGM
                     GVVDRLKPDWREFFSVYGDGTIDLRTAFKDTLIAVTGASESDVTNYISRRDGADGIPG
                     TEDDQRISDSEAYRLLGLSGDRGRALSSILTSEDSVRRITSTGYVGEKRAQIIVVARR
                     GEDRSLTYLARIEE"
     gene            1198..2412
                     /locus_tag="PLP58_00510"
     CDS             1198..2412
                     /locus_tag="PLP58_00510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ07031.1"
                     /translation="MSISASILLLPAPDTTWRVWKPRASSADASVEAPSEAAGKGKAF
                     VIGLPATACRTIGLVLPNADHEVLEQIIITQLERRGLKLHAVTPRNFRWHLLTQTAST
                     ATVSVDVLADPFPESLALTDATDYTAALRMVNLPAGQIIVTEEHGSLVLVAGFQGKLY
                     HSHLFAPASAAAEEIAQEIVLARLALEQDLGAGSFSGITLAGASWDANATRPLQELTG
                     LPVRIVAQLPPNSDLDTSHWTRLLPAGVHAAQSARARAAKLLRFGVLAGMMVVALGFF
                     AFAYLRHLDGTAAALEKDVAATSEAAGKVKATATHWKALSPAIEPRRYPLVLLAEITK
                     LMPPSGIVIRDCDIRTTEIDIRGEARDAQLAFQFVEDLQKHPVLGLYTWSKPQPTVRE
                     KTAQFRAQGKMQ"
     gene            2451..2999
                     /locus_tag="PLP58_00515"
     CDS             2451..2999
                     /locus_tag="PLP58_00515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ07032.1"
                     /translation="MQQKLTAREKNLLLLCVGVLVFMALAITANGFMQKRSALLQKIA
                     AIEVQKRENDTWMADRAFWEKRRSWLEEKMPSTDSLGRAQGQLLEQLQNEALDLGIAI
                     QQQTLPEAVTTPDYREVTVNLRVRGDQTAVLGWLATMQSPEKFQAIKQLDMELDSRAR
                     ETTPQTLCNLTLARWFKPETGL"
     gene            3020..3667
                     /locus_tag="PLP58_00520"
     CDS             3020..3667
                     /locus_tag="PLP58_00520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ07033.1"
                     /translation="MKNRLFISLASLPILVAAQNADPDLPQAFDPKSVAPVLESSPFS
                     RMVNLSDNLVLTGIAYVDGKPVATIFNKETKQSSVVGSEPNFQGWILQEALPAPDVKL
                     SQAKISIGGEVVSVRYATVSAEEMRGERRRDGERGGPPPPGGEGDRFRGPSRGPSEED
                     RRRYEALSEKGKEKLRDMMRSKFSDEKFRNAPEEERRNIIRREVERIEKEDQGGR"
     gene            3865..6399
                     /locus_tag="PLP58_00525"
     CDS             3865..6399
                     /locus_tag="PLP58_00525"
                     /inference="COORDINATES: protein motif:HMM:NF012485.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="secretin N-terminal domain-containing protein"
                     /protein_id="HRJ07034.1"
                     /translation="MPADPAAQQAPPAGDQPSGPPTGAPPGGFGPGGFRGRRGEGGFG
                     GAPGGGGGAGGGIASTLSETIRMDGDQIVLQFPNNPVADMLSIYELLTNITLIKDTTI
                     FEGPQVSLATPKPVSKEEAVKLIEATLFTNGYAIVMEPDGRSARILPARTQSANSVQF
                     SQGVNFYTSPLDLPEGETLVSYFMRLNHLDPEEAARMLSGHVGLGIYGRITPVTTPPG
                     LLITENSSIVWQLINIKEAIDIADTGSALLTKFIPVEYADAATVAQIIQATLDAQLQE
                     EETRGTNTFRGSPVQDNRSNQGGGDQRSSSPQPSSQNFPGSSNRSSSQDNSRSTAPKP
                     KAQVVADTRLNQILVVSSPDDYTYIASMIAEFDKVLPVDEPYERKLKYASAVDVLSAI
                     ADLLQDTGGGATQLPGGGTLQQQRNQVLTSTSSQLLTGRTTTGTRGGQLLATGGADDT
                     ATGGTGTVSRADLIQGPTEDNAPISVLIGKTRVVADPMSNSILVMGRKDAVDKVNGLL
                     DKLDRKPAQVYLSTVIGQLTLGDGFEFGVDYLSALTSKEGTNFSGSSFVSRQDVLSGN
                     AVTRAVRDLRDNVITTPFGPAAGMNLYGAIGDSLEVFVTALEATNRFRVLSRPSVFAL
                     NNKKATITSGQLIPVPAQTISVPGNNNNVNGSVTTTVEYRDVVLKLEVVPLINSDGEV
                     QLTIAQVNDTVIGTQRVEPNDIPIIGTEQIITSVTVPDRNTIVLGGLISEQEKRDTQG
                     MPFLSRIPGVGNLFKDNKDSTNRSELIIFIQPQVINDNNALRFASMREDVRTKVGADA
                     AEKFPQQPVIPTPTTAPGDTPQKKGIFSRIFSSKRASTPLPGRQRQ"
     gene            complement(6441..7355)
                     /locus_tag="PLP58_00530"
     CDS             complement(6441..7355)
                     /locus_tag="PLP58_00530"
                     /inference="COORDINATES: protein motif:HMM:NF012472.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HRJ07035.1"
                     /translation="MTPRLKLSPNGPEFSRLIYGTWRILDDPATANREDLLRRLRACL
                     DLGITTLDTAEIYGLYKVEEFIGQALQADPSLRDKFEIVTKAGIYVPCEFHPQRKTAY
                     YNATAERLVKSAEKSLRLLGTDRIDLFLVHRPDWLTSADETAEGLNRLLREGKILHAG
                     VSNYNTHQFELLNSRMERPLVTNQIEFSLLHMAPIYDGTADQCQRLNVRPMAWSPLGQ
                     GRLMDKADPAAARIHARCAELSAKYGHATVDQLAYAWVMAHPSKPLPIIGTNKLERLA
                     TTARAAGIELEREDWYALWEAAQGCQIP"
     gene            complement(7470..7742)
                     /gene="rpsT"
                     /locus_tag="PLP58_00535"
     CDS             complement(7470..7742)
                     /gene="rpsT"
                     /locus_tag="PLP58_00535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009964375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HRJ07036.1"
                     /translation="MANIRSSEKDIRKTVKRTAHNQAVKSRVRTLRKKVLAAIESGDT
                     AAANASLGEFASAADKAAKTNVLHKNTSSRLKSRLAQKIGSLAKNA"
     gene            7881..9434
                     /locus_tag="PLP58_00540"
     CDS             7881..9434
                     /locus_tag="PLP58_00540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007417272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4080 domain-containing protein"
                     /protein_id="HRJ07037.1"
                     /translation="MPGPQMLAWSVMPDIVLATLNAKYIHSSFGLRCLMANLGGLRER
                     AVLREFVIQQQPLEIVEAVLAEDPKIVGFGVYIWNVTQTTAVVALLKRLRPDLVVIVG
                     GPEVSHETESQEITALANHVITGEADIKFAEVCRALLDGTDAEALPRVMPAELPPLAA
                     LASPYDLYTDEDLTHRVIYVEASRGCPFTCEFCLSSLDIPVRAFPLESFLFSMQGLLD
                     RGAAQFKFVDRTFNLHLPASTAILRFFLERWREGLFLHFEMVPDRLPVQLRELISRFP
                     KGAVQFEVGIQTFDETVSKNISRRQNLERLEDNFRFLRQETGVHIHADLIAGLPGESV
                     ESFGLGFDRLVRLEPQEIQAGILKRLRGTPITRHDAEYRMVYSPHPPYEILSTRDISF
                     EEMQTMRRFARYWDLVANSGQFTGTLQLLWRGAASPFQRFMRFSGWLHARLGRTHQIA
                     LHVLARELHEFLTGELGVDRDLAAEALETDWSRAPGRGALNLRGGSMTEIKPASGRIA
                     RRQSRHMRE"
     gene            9515..10591
                     /gene="rlmN"
                     /locus_tag="PLP58_00545"
     CDS             9515..10591
                     /gene="rlmN"
                     /locus_tag="PLP58_00545"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: protein motif:HMM:TIGR00048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016433 - rRNA (adenine) methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HRJ07038.1"
                     /translation="MPIYLHELGFDELRARLAADGLRPAHAATLWNALQYRAQTDLLA
                     REDFPPPLRRWLERRLGVQCLLDMPEVSSHTPSSDGLTDKFLLRLQDGQEIETVVMGY
                     EGRHTACVSTQAGCAMGCVFCATGQMGFARHLRPGEIVAQVLHARRVLQGAGRRLRNL
                     VLMGMGEPLHNYDNVMKALQILSDTRGCNIGPSKMSISTVGVVPGILRLAEEDRPYNL
                     AVSLHGSTEEERAALIPANQRWPLHELVQSCRTYGEKTGRKIFFAWTLIAGVNDSPAH
                     ARRVAALLRGLRAHVNLIPLNETAGYAGRECAESSASVFHRIIQDAGIPCTIRQKRGI
                     DVAAGCGQLKAARRKRERAAPQAA"
     gene            complement(10661..11062)
                     /gene="acpS"
                     /locus_tag="PLP58_00550"
     CDS             complement(10661..11062)
                     /gene="acpS"
                     /locus_tag="PLP58_00550"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HRJ07039.1"
                     /translation="MKLEDSCVSIKGAGIDLVEVPRIRSILEKHAERFVGRTFTDGER
                     AYCESCADPAMHFAARFAAKEAAAKALGTGVWAEGVDWKDFEVTRAANGRPGLLLHGA
                     ALARADALGVRAAHLSLSHTRELAIAQVIFE"
     gene            complement(11059..11805)
                     /locus_tag="PLP58_00555"
     CDS             complement(11059..11805)
                     /locus_tag="PLP58_00555"
                     /EC_number="2.6.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012420247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033856 - pyridoxine 5'-phosphate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine 5'-phosphate synthase"
                     /protein_id="HRJ07040.1"
                     /translation="MPLHLGVNIDHVATLRQARYRTMPDAHNVEPCILDAALECEAAG
                     ADSITIHLRADRRHIQDADVFHLREKIGTKLNLEMGNTAEILEIALRVKPDFVCMVPE
                     NREEVTTEGGLDVAGQMEALRGTVRQLENNGTRVSMFIDPDEEQVRASAESGASMVEL
                     HTGTFANETGEARAAETDRLRAAALLAHSLGLRVNAGHGLTTRNLPELFTVPHLHELN
                     IGHSLVCRAVFTGLRQAVAEFRSVMNTYAL"
     gene            complement(11811..13349)
                     /gene="purH"
                     /locus_tag="PLP58_00560"
     CDS             complement(11811..13349)
                     /gene="purH"
                     /locus_tag="PLP58_00560"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009960796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0003937 - IMP cyclohydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0004643 -
                     phosphoribosylaminoimidazolecarboxamide formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HRJ07041.1"
                     /translation="MPIARALLSVSDKSGLLDFAKGLAALGVELLSTGGTAKHLKDAG
                     LSVVDVSEYTGFPEMFEGRVKTLHPKVHGGLLQRRDNDEDKRNAAKHSIPVIDLVVVN
                     LYPFEQTVARPDVSLEEAIENIDIGGPSMLRSAAKNHRWVTVVCDPSDYAGVLGEMKE
                     NEGGTKMATRERLACKVFQRTAAYDAAIAAYLNKEQTTQKTFSLSLPLYSELRYGDNP
                     HQAAALYGNFGDYFTKLHGKDLSYTNVLDIQGAAALIHEFRRPTLAILKHTNPCGVGS
                     AEEDLREAWTKAFETDKQAPFGGVIVINRSMPLSLARIISEIFTDVIIAPDFDADARA
                     HLQKKKNLRLIQMHPSVVEALREPVIRNAPGGVMVMDSDPRALGLEDLENKVKTQRPP
                     TREEIEAMRFAWRVVKHVKSNAIVFASHDRTLGVGAGQMSRVDSCRIAIWKAKEAGLS
                     LDGSVVASDAMFPFADGLIACAEAGATAAIQPGGSVRDEEVIAAADERKMAMIFTGHR
                     HFLH"
     gene            complement(13430..15646)
                     /locus_tag="PLP58_00565"
     CDS             complement(13430..15646)
                     /locus_tag="PLP58_00565"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HRJ07042.1"
                     /translation="MAIVKAAVGGLERLFFALEMPGKPDEVPRPMTHSFRKIARISGL
                     AGLTALAAVNAGAQASLEERRQAARELEQRLPKPEAAIAGTGDMAAARQARLRQLLPE
                     STRRLLQREPFHLLMLGDAAPVWPEEGDSPGGAPAFDEVFAHDLAALFFYTGGVRIAE
                     KDQPGVELGPALVLRNLSRPGGSVLDAPGILASTARQTPVNLVLLCLGQAEAGMAPVV
                     FSRAVREAVSAAREMGAEVILASSWPPMSAASEHVLGLARPAADVLAEIAVEHGLPFV
                     DLGGLRGIMDAPPLEAGQDVAHWFAEVERVYRGFFHDSGAGFIPRASLHGRLGAALAD
                     QLLDGPPAVPWSMGKCEAELDAQGRGLRVSWMLENTTKEPLDLIALPLIAGGWRPVEA
                     VPAVRIKPGESTELEATFARAQPAAVDEPLVRLPVLLSGGGSLEIAGLRCRLQPAAVV
                     WEMETFFNQESAISPACVLVNHGKRPLKGSWQAGFNGATLKGQYDVPAGASQTLDIRF
                     ELPSDASGRALPLSLRLEGDFKHESTRSLRLTPNLGLGTARPLAASDPALDGKAALTA
                     HADASTLTLMLDLADSGLLTDSPAGPAWELLLRLDARSYGKRLESGAAAALRVSGPAV
                     DGPGKVDHVPAWAFGTGYAAGFDPAEFKAELLTLKESRRLIRLTVPRTYLYLHEWALE
                     NGNSQLGINLRLRLNGGDGGAVFDLSPTRKPSDAVDALVVLELAKQPTRRFTVSVE"
     gene            15691..16242
                     /locus_tag="PLP58_00570"
     CDS             15691..16242
                     /locus_tag="PLP58_00570"
                     /EC_number="2.7.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018970339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046316 - gluconokinase activity [Evidence
                     IEA];
                     GO_process: GO:0046177 - D-gluconate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gluconokinase"
                     /protein_id="HRJ07043.1"
                     /translation="MPDASATTLHPALPGTLVIMGVCGCGKSLIGAKLAEKLGGVFED
                     ADDFHPPENKAKMNAGIPLTDEDRWPWFDALRSHIEDMRGRTTVFVLACSALKAAYRE
                     RLRGGDDSGGLVFIHLKGPRGVIFERMSARQGHYMPVSLLDSQLAILEETPDLWTVSL
                     EQEPGEVVDEILRRLREPGIAGA"
     gene            16239..>16730
                     /locus_tag="PLP58_00575"
     CDS             16239..>16730
                     /locus_tag="PLP58_00575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ07044.1"
                     /translation="MSAPVWHRSLAARHALAMLACVAGGSILLLAWLGRMQHTESASE
                     AAALAKNHADFVRRLNLPRSARLAGDLRELLGLDIYFRSMTGALDPMPGGDLKPALME
                     ARTGTDWQRLPDGRQALALSLDETHDMIFVRAEPPLALSLRHPVMRHALPAFWLLAAL
                     MAWL"
     assembly_gap    16731..16740
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <16741..17634
                     /locus_tag="PLP58_00580"
     CDS             <16741..17634
                     /locus_tag="PLP58_00580"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HRJ07045.1"
                     /translation="RVTKPRSANSAAYCRFGARQVVRPLTGLTRMLPGFFSKDGPMPA
                     EAARQDEIGLLARALSRARADLHQERRRREESERLALLGRVATGLAHEIKNPLASIQL
                     HAQLLDSASHNAETASSLKHVIEEARVIEGLVNQWLFLARPSPPEKAPLELRGLLCAT
                     LDSVRLQARHAGVRMNLEPGPEVTVPGDRVRLGQAFRNVILNAIQAMPGGGLLSVRLQ
                     SEGTHARLVFHDSGPGFSRAALQQATGLFYSEKEGGMGVGLNVVEEIVSAHQGSLRIK
                     NHPKGGAVVELELPCGADGDS"
     gene            17640..18932
                     /locus_tag="PLP58_00585"
     CDS             17640..18932
                     /locus_tag="PLP58_00585"
                     /inference="COORDINATES: protein motif:HMM:NF025885.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HRJ07046.1"
                     /translation="MPRVLIIEDDHALAAALAVAARRLGAEPAVSASGQGGLEKAGRA
                     AFDAVLLDIGLPDMSGLRVLETLKARGCSAPVLVITAHGTLDHALEARRLGAHEYFLK
                     PLNLADLQSCLRALLKPDKPQQPAPAAPRPLMIGSAPAMQRAFAVVAQACATREPVLI
                     TGAPGTGKTLAAEVIARHSADSSPLKIFRCDEWPDGKARAAFHEALAGEGPLLIEEAA
                     SLPLPLQGDLSAALQENTRRILATTSADLPVKIAAGLFREDLHYQLGVLRLRLPPLSA
                     RTEDVPALASYLLASASPGRDMLVDPAAMSLLKGHDWPGNVRELAGAMRHASAVCAGT
                     VILPHHLPESVASARAAGGAVLEENLRQALTAWLDQRLTAGGESTPDYDTLLADIEHW
                     MLSELLERHEGRPTHLAAALRMNRATLRKKLRELPGAD"
     gene            complement(18955..19674)
                     /locus_tag="PLP58_00590"
     CDS             complement(18955..19674)
                     /locus_tag="PLP58_00590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009961817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRJ07047.1"
                     /translation="MIQIENLTMQYGDLRALDELSLEVRPGELFAFLGPNGAGKTTAI
                     RLLTGLMKPLSGSVRLCGFDIQSEPMKAKALLGYVPDVAAFYEKLSAPEFMHFIAELF
                     EMDPARAAERTEALFTRFALHEHSGQRIENLSHGTRQRLAIASALLHEPKVFVIDEPM
                     VGLDPIHARVVKEELKNLSRSGATVLMSTHLLNIAEELADRIGILKRGRLVFDGTMAE
                     LRATREKEGQSLEELFLEMVE"
     gene            19827..21254
                     /locus_tag="PLP58_00595"
     CDS             19827..21254
                     /locus_tag="PLP58_00595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009962053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1501 domain-containing protein"
                     /protein_id="HRJ07048.1"
                     /translation="MRTPYFPCAGARALHAPTRREFVYGLGASLGSVAFSSMMAAEKS
                     PGPLAPKPQQAHAKAKNCIFLMMEGGPSHIDCFDPKPALEKLHLKEFIREGRQKSAME
                     SGKRYYVRSPFKFSQHGQSGAWMSEPWRHLAGVADELCFFRGCQVDSVNHPTAMYQMN
                     CGNRFGGDPAIGAWVTYGLGSVNQDLPGFVVLPEISYPQGGSANWGNGYLPAHFQGTP
                     LRAKGSPILDLQPPPGITQEHQRANLDLLAKINDRHAREHPWHDELKARMNNYELAFR
                     MQTQVPEVLDLAGETEKTREMYGIGRDATDAFGRKCLLARKLVENGVRFVQLYAGTWD
                     SHDYIERAHGNLIGQVDQPIAALIKDLKQRGLLDDTLIVWCGEFGRTPDNGVRGGTAY
                     GRDHNPSAMTIWLAGGGVKAGHTIGLTDDTGMEAVECVHHVRDLHVTLLRLLGLDDNK
                     LTFYHAGRFKQLSQFGGQVIQELIA"
     gene            complement(21267..22418)
                     /locus_tag="PLP58_00600"
     CDS             complement(21267..22418)
                     /locus_tag="PLP58_00600"
                     /inference="COORDINATES: protein motif:HMM:NF024753.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HRJ07049.1"
                     /translation="MTALRIAPAFLLAAFSCHAQEAGDWIHARGDEAMRGVSPVELRF
                     PMELAWQYRAVPVEKARGEMLVSGAVARGGRVYAGCKEGRFFCLDLKTGNKIWEAEGG
                     GAFDGAASFAGEIVVAGCQDGFVYAWNAETGKEVWKFETQAEIHAASNTFTHPETKEQ
                     KILIGSYDYSVYCLDAKTGKKEWAAETGYYINGGAAIGEGVVVFGGCDSVLHVHDVLT
                     GEEKRQIEVGAYIGNNAAIADGVVYVSHYGNRVGAYSLEDGMAVWEYGEREFEFYAAP
                     AIWEKAVYLGGRDRRFHAIDRITGKGLWEYRCRGRVDSSAVVCAGKAVIFGGDDGYVY
                     ALDLKEGKELWQYEIGAAVKTSPAVAGDFVIAGADDGVIYAFKNAPAKP"
     gene            complement(22442..23254)
                     /locus_tag="PLP58_00605"
     CDS             complement(22442..23254)
                     /locus_tag="PLP58_00605"
                     /inference="COORDINATES: protein motif:HMM:NF018178.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1080 domain-containing protein"
                     /protein_id="HRJ07050.1"
                     /translation="MKKLTLTSLALASFAAFAQEKPTPTGYSDTPVIPGSQWKVHDID
                     RPHPAAVTPSEKPGGPPSDAIVLFDGTSTEAFKAKKKGPDGKPTAEWGPCEWKIENGE
                     LLVDGGDAWTNQEFASCQFHLEWKSEPHTAGNSQKKGNGGVFFMDLYESQILDCDNNP
                     TYADGMAGAVYGQTPPLVNAVRKAGQWQSYDIVFTAPKRDEAGKVLEPARITTFINGI
                     LVQNNTVIQGPTKHKNITSYEGVFPAKASIRLQDHKNVPPVRMRNIWIRPLD"
     gene            complement(23329..25182)
                     /locus_tag="PLP58_00610"
     CDS             complement(23329..25182)
                     /locus_tag="PLP58_00610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009962692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HRJ07051.1"
                     /translation="MKTATALEAKAPLIDTSKMSAGQKAALEMAEAARDERHNTGLAA
                     GIFFGGPDFSKVMPFPRQSDEDKDQGDAFLGRLRAVLAGHADPDAIDRTGEIPDKLIK
                     ELAKLGAFGIKIPVKHGGLGLSQTNYSRAAMLLGGECGNLAALLSAHQSIGAPQPLIL
                     FGTEEQKAKYLPRCAKGEISAFALTEKDVGSDPARMTTTAVPDGDDFIINGEKLWCTN
                     SLKAGLIVVMARTPTPEKPHATSAFIVETSWPGVEILHRCRFMGLRALYNGVVRFKDV
                     RVPRENILGGEGRGLKVALTTLNTGRITLPAACAGLARRCLEISSTWAAERVQWGQPV
                     GRHAAISEKLARMTADTFAMESVVHYVSALVDRDKHADVRLEAALAKLWGSEHAWQIV
                     DDTMQIRGGRGYETADSLRARGERPDPVERLFRDCRINTIFEGSSEIMRLFIAREALD
                     PHLKIGASAVNTTLPLKTRARAAMKAGLFYARWYPSLWLPQGPGDAADSLHPALRGDL
                     AEIHRQSRRLARALFHAMVLNGPALERRQVLLGRLVDIGAELFVWSATLARAESLITD
                     SALTSDDVDRLLRKVRYFGKLTRQRIREHHSAMRSGLEKDAVKLARDIVGA"
     gene            complement(25329..26033)
                     /locus_tag="PLP58_00615"
     CDS             complement(25329..26033)
                     /locus_tag="PLP58_00615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ07052.1"
                     /translation="MIRRIRTALSLPFFIGCVINAQVAAPLLGLAPDKNRLPAHVTRL
                     TRRKTATVYKFATDPQNSIVKIIYRRKARDYLRFLDERHADREHKSALALAEIGLRVP
                     EPRGSFSNWNPFSKVDSIYVAEFMEGLQPGKIFLRPDLTAPDIRRVFIRKYAAGMRLM
                     LDSGLHNLDAHPGNVMWNGDPDEDLVWIDNDVETFPPEGRPAARQSILDLLEWKVRRD
                     ESGLTLDEVAEIARII"
     gene            complement(26115..27149)
                     /locus_tag="PLP58_00620"
     CDS             complement(26115..27149)
                     /locus_tag="PLP58_00620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009964019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHMP kinase"
                     /protein_id="HRJ07053.1"
                     /translation="MDSRTSTPIITQAHARAGLVGNPSDGYFGKTISFIIRNFRAEVR
                     LEESEELRIEPNERDHSVFGSIEALARDVRQFGYYGGIRLLKASVKRFFDHCAKNRIR
                     LHGRNFTLRYSSNVPPQVGMAGSSAIITACWRALMRFYAVEIPRHVLPGLVLSVENDE
                     LGIPAGLQDRVIQSYEGVVFMDFNRASMEKLGHGHYEELDPALLPKLYVAYTTRLSEG
                     TEVFHNDIRGRWNRGERDVVSAMYHWAGLAQRVRDMLLEGRGHEIGPLLNENFDLRRR
                     LYQLSEGNIKMVETARDCGASAKFTGSGGAIVGTYEDEAMYQRLQAALVPQDVQVIKP
                     LILSTGDIPG"
     gene            complement(27216..28631)
                     /locus_tag="PLP58_00625"
     CDS             complement(27216..28631)
                     /locus_tag="PLP58_00625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007417184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1501 domain-containing protein"
                     /protein_id="HRJ07054.1"
                     /translation="MHSSPHFLTVTRRHFLTHAGAGMGAAALGSLLARDLPASTPVTG
                     PHFAPRAKRVIYLHMIGAPSHLDLFDHKPVLQKHDGEKCPEDLLKGRRFAFIGGEMTL
                     AGSTYQFKKHGQSGAELSELLPHLATVADDICLLKGMHSNEINHAPAQMFLHTGFGQG
                     GRPSLGAWTTYGLGADNQDLPAYVVLLSGPPGGAGTSLWGTGFLPSRYQGVQFRGSGE
                     PVLFLNNPEGHSRQDRRQVLDTVRALNEERLGIVGDPEIATRISQYEMAYRMQASVPE
                     LMDISQESDATLEMYGAKPGAASFASNCLLARRLVERGVRMVQLYDSDWDHHGNLDKR
                     LRAKTRDVDQPMAALIRDLKQRGLLEDTLVVWGSEFGRTPLRQGIDGQGTKTSPGRDH
                     HKDAFTFWLAGGGVKAGITHGRTDELGFGVVEGGVHVHDLNATLLHLMGLDHERLTFR
                     YQGRDFRLTDVHGELVSAILA"
     gene            28775..34837
                     /locus_tag="PLP58_00630"
     CDS             28775..34837
                     /locus_tag="PLP58_00630"
                     /inference="COORDINATES: protein motif:HMM:NF024371.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3857 domain-containing protein"
                     /protein_id="HRJ07055.1"
                     /translation="MILALAAAPPSSAQEAGKGGAAPASALRLSRKAPVVYERQLKAL
                     LTPAEKVLARRGDFETNGADGVILLDETLIHSEPDGRRVLVYHRMFQALNESGARMTA
                     EDTFSFRTLDQRIHLAEAQSLPPEGSAVEVEDGSVIMESPQHDAADSVYGDRGQMRII
                     FPAVKPGTVTRMVVVIEEFDERIPGHFSTWESWGAFWPTRHARTVLHLPEDMEARLRE
                     TRLGAGVPEPVKSSAAPGWRTWTYDKHRIKPDRAEHGRPPKSQSGPAVFLTTLPDWET
                     FGDWYRALLRERAEVTEEMAALAREWTRAAGTDEEKAAAILGHVARDIRYTGLEFGLG
                     ALQPRAPVEVWKSAFGDCKDKSNLAALLLRSLGIEAHLALIQTEHLGRVERRSPDSRH
                     FNHAIVALRTAQGWQFTDPTLRHVTPGMLAPSSSGRDTLIVKPDCIEWARTPVFEGGL
                     VHYHVDAERAEDGSTEGWLECRAGGFYMATDRAYHENMSREEWKRDAQQTLAGLLPGA
                     QIIDLDTDRSAPDVLWRAYFSQPGRASGGDERVPASFPAGEPVMLSMGQDETRETTRF
                     LWPITWRVTSAVRLPAGWTAAETPPPFDLHTEAYEVEARWEPGSGLLRTFYEGRVPRG
                     DLTPAAHQVVWRGAQSLDAWLQRPLWLARSGETPPAPSPEAAITLGKFPLMPTGEGQL
                     ALVDRRYPENGNLDLRRAALRKVLEFFPDDPVTVFNTRTRLATADWEQDKHAEAEKAL
                     REILAAPSPKITPEILAWGRYLRALVLMDLKQHEEAARTFEGLAGDTTLSAYRRAWAG
                     FQLSRARAELDQKEGALAAARAGLLLEQAESTPPLLARAAMHLLELDRQQELPPLLAS
                     LIERGEDLSVPALTALAQLVAGLHEDGKSAQAATLLDILEKLDFINEGFARALAEAGN
                     ALRGGAVAAALQKDLQAWLEARPDTPALKPPPDGWPQSREECAKAFEAADNAASSDLG
                     HRLALRLLTAFEPGTDFPRLLWRAAAHLEHHERQSQPSQPSDLMRFLLDLGLRLPRAD
                     DHYWEMRYLQGVVLENLVQDKAAAAKHFSTLADENDMPPAYQPSFIVRAAACHEHLRE
                     WEKAAGTWSRLERLTQFGSSGDGLLRAAHLRLEQGNVDEALRLLSLAGGSRDFYLRHT
                     GMKETLEAMLQFADEPALVRATWSLEPAWWPDWKKLRDTLGILESDIEPAIHDVAAFG
                     AEISQAVQEKDAARAGELFRQLAHNARWVPAHVINTVWASLYRMEDLHPRHVRELKLF
                     NLSLLESGRVFTEDNRRARSLYSVMCFLDTDQQEAALNHIGEYFNAHPRDDHAISFTM
                     SRLWALAAVEEKNERPACITRLRQDLAAPALRQDRMASVRQLVRLLRLEDMQSEIRPL
                     LEGELSHPAHSQDQEALAALKDMLRSETEGAAVTRTVQRWLERHAPPWFDAVQPLSLA
                     DAGLDDEDADLDEEADNLPPVPRCKLWLLAAQETSLDAEDRVRWFANALSLWPRLNAL
                     TRDQFHAGVSTLLDDPQAPADLKDRALRVTAIVCADHDEKETWLRFSKKHDDLILDEY
                     TRDVLNVVDLVFETGPASAASPAASTRAVQTILKEKDSYLLELLVEWLADRALRHGRP
                     DAAQAVADALAPARKPAGFDASELQTLRLGLVKKLSAVKRLQPVHEALARIVLEEPPR
                     EQLKGVPDDCDIFGGADYDALGSARWLAVFLHELRHDRHERQDLTDWHTLASVQSELD
                     AAGTASLRRRLISAMTTAPVEDDTARADLASLAEDLTDLDSETERAHLQESLQPWREA
                     RDTAAHSAIRLIEARIALRTGGPLDVDAMLRQIKDPDSQTELRWMQLRRARATEDAAL
                     ARKALDALGADALLDPGRMHIVIPALKHTNRQDELALAVEATEAAFQEALGRAWTGRH
                     SGAARYALELAGLLGQHENLPPGWFDFCLRAYPERMDQLNLECHLAQMRGDWTAVLAA
                     AEELVGKYPTFYSHYWPQARALWKLGRAAEAREPLAIFLRHCHDEPEHAEAGRMLSEL
                     DSKR"
     gene            complement(34934..36388)
                     /locus_tag="PLP58_00635"
     CDS             complement(34934..36388)
                     /locus_tag="PLP58_00635"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase"
                     /protein_id="HRJ07056.1"
                     /translation="MKLHIFLTLAAVVSLSALDRPNVILIVADDMGYADAGCFGSQTI
                     RTPHLDRMAAEGSKFTSFYVAQAVCTASRAALMTGCYPNRIGMQGALNHTSKEGVHPG
                     ERMMPEVFKAGGYATAAFGKWHLGTVMKFHPCRRGFDEFWGIPYSNDNSKFHPSLAAE
                     MPPLPLYEDERVIETDPDQSQFTRRITEKSVSFIRRHKDKPFFLYVPHIMPHVPIFAS
                     ARFKGGSPHGLYADVVEELDWSVGMILEAVHQAGVDDKTLVIFFSDNGPFLSYGNHAG
                     SAAPLREGKLTTFEGGVRVPCIMRWPGHVPGGRELDTLVTAMDLMPTFSAWLGAEPPA
                     LKIDGRDISDVLLGKDGAESPHEAFAFYAGKEFQAIRSGDWKLHFEHPYITPHETPGK
                     DGKPSNWENMTPASITQSGIEGIATRHGYKVASQPLALYNLRADIGETTDVSAAHPDV
                     VERLQKLAEPIRHELGDTLTNTPAAAARPQGREE"
     gene            36642..55910
                     /locus_tag="PLP58_00640"
     CDS             36642..55910
                     /locus_tag="PLP58_00640"
                     /inference="COORDINATES: protein motif:HMM:NF024777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FG-GAP-like repeat-containing protein"
                     /protein_id="HRJ07057.1"
                     /translation="MKKIQTNLGGVAALMAWMMTAAQAFSQSQLGLTESPPASGFYSN
                     VVNDSLTGIGVANAKSWLVELEGGDRVTIWAVTSAVGSNPRLRLYNAGGTLQASNNGT
                     QQGETMIQSFTVTTPGSYTIQFYADSVASAFQMRVDMGRGFALESEPNDTQYTPTLPT
                     LTPTGGGFTTKAAGVLKEGADYFDLGTLAAGNSVNVSLGLPALSTLAAGDVEMTLFAK
                     GSEGSPVATTFGAVLNGAIPSSAEYVLRLRVNPELSGHSLRFDGTNDDVALGNPNELR
                     ITGDQTIEMWIKPDDFGGRRNPWNKAYGGEGTMTIETDGGINYFYGTNGGDNNPYQGF
                     FSNKLMTAGEWTHIALVRRLDSEPRKLFWYFNGKLVNEVNASYFPAVAGALNAVIGSG
                     YAGRFRGLIDEVRVWNVARSAAEIAANLDNELNGNEAGLAAYYKFNEGSGAALADATA
                     NALNGVINGGAEWAGTAGAGAFSAARTGLMAQYVASVTVTDAAIPSVVRAAPVPVFGH
                     TFEGAPDLIMNPGSMTAYRGQNGTSFHVQVTGGGGSVWGTGVYSDDSALASAVRHAGL
                     LANGETGLVTVTILPGGSSYTGTPQNGVSSSNKGSHPGSYSLAAYSGPAPALNAVFAG
                     MRVDFSERMLPSTLQNAANVILTHAGADGVLDTADDHAHTLSNSYDGLATTAYFTVVN
                     EPLQPGLHRLRLTTGVQDRAGNGLAADSDTLFTVTAKTPFVVENRDNNDRLKATPLNA
                     PAAAVFDRSFTYTHDIAAGTGPHRLRLLDLDGDGAKEAIVAAHNSNQIRVYPGIANGS
                     FDPTSTNYATGSNPWALELLDYDNDGLLDVIVVCHSSDQVRLHRNNGDGTLSADGSIT
                     VGDGPIHIVKADFDEDTITDLAVANFNTGTGGRSISILLADGSGSFTESKVSVAGNVF
                     RPYGFSAGDVNGDGHDDLVVGDFDTDMMRVFQGNGDGTFDAPVDFAISGVNPTSIGLA
                     DFDHDGNLDAMVMAETSTLVSILKGNGDGTFQAFATWPLAVNASQYFAEAVDVDEDGW
                     VDLVIPRTNGLEVVYNSRSTVAPAFTGALRYQIGYALGFVLQDVNGDSLPDLVVNEWN
                     SNRIRTLLGNPQIPLTQDAILARLGNTHGRGRILDGDVDWFSFTIDGPKRVVLAADIP
                     FNPGSAGLNYNIYDADGAHITGFNAASNGIGQTAQLNLNAPGTYYVRVAHNWGYTGEY
                     RFRVSAFDVPTTIESEANNSIGQADSVALTLGVGSLTGAAAGYIAGDDTSGDYWSLGN
                     LGAGAEITLDLRIPQSSPLSGRMAIFKTDGTQVAVSDAGDPQLSYAVTAGNESAYYFR
                     VFDAASTRGLHSEYLVDVTITDPEQPFVTAVSLPAEGATVSYAGPNFTVTFSEDMLAS
                     TVTNAANYDLRAAGVDGVFGTADDDVYTVVPQAYSAGLSASYHIPDGPLQPGNYRFIA
                     GTGLTDKFANPLLSAHTRLFTVAGVPGYVFESRSNETLQTADSISTSPGAAHDGSFTS
                     AGFTATGNGPWGLKLLDLDGDGHKEAIIALHNAAQISVLPGNPDGTFGAAVNYASGTN
                     PWDIELVDVNSDGRLDVVVSCHGSDQVRIYNNPGDGTLTAGPVIPVGDGPIHMVKGNF
                     NGDAFTDLAVVNHLTGTGGRSISILLGDGAGSFTESKITVSGQTVQFYALTAGDFNGD
                     GTDDLAAGDYVLDDMAVFLNTGANNGTFAQPVFYPVEDTEPTGAGVADFDGDGHLDMA
                     VCTEFHNRVSVLKGRGDGTFEPFTTFDVDGNSNMFYVESPDLNGDGWPDLLIARYNGV
                     VVCQNRGDGTINFSAPVRYSDSSNTGGLAWSDVNGDGRVDLLANSYNQNRLYVWTGNS
                     SQPLAEDAVVAGMRHGALRGHLTTDSDVDWYSFSALVQDRLLITTETPGAQGSSGRNY
                     NIYNSVGTHIGGFNANSNGYGEWTVTLPQSGTYYLRVAPNWGGTAEYRARLTLARPPV
                     FAEVESNNSVSQFNTPPLALSAGQLTTTVLGVNMPPDTSGDFFNLGNLTPGTQVTVSV
                     LAPQLAVDNLPSMQALELKHRWSFGEAVGATEFTDSVTSLPSANLLGTGAVVGGGQVV
                     LPGGASGSAPYIDLPNGIVSSRTGSATFEGWATINGSQNWSRLFDFGTGTAGELLSPG
                     GSATGGQHFFLSAQVSGTLTSNRISINSGASNSADFGVSYSAGQQFHYAVVYESAGAS
                     GGVIKYYRDGVLRGSFGTAAMLTGIADLNNWLGRSNWTSDGNLNGAYSEFRVWDGAFN
                     DAQAAASAAAGPDTVAGAVPPALAAQPFLTLFKSDGTQMAFAAPGTGPLQFTLGASDA
                     ASYRLRVHRNERSVTSLYQASIIIEDVTPPFITSNSLPAEGSTVTFPHPVFTLGFSED
                     MLASTVVNAANYELRSAGPNGVFDDADDVPYTLSPSSYTSGLSATLRIMDGPLQAGAY
                     RFTAGTGLKDLFERPMDASHVRFFNIQDVAGFVTEGRNNNTLATAEILSGITTPGGFD
                     GSYAVQPSFTSGGSAPISLLLVDLNGDGEKDLVVSNRDSNNITTRLGAGDGTFGAPVT
                     YATGSQPHYMCLVDFDKDGLMDVVVPIYNADQTTIFRNNGNGTLTLHATLAAGDGPLS
                     AAAGDFNGDGNPDFAVTNHLTGVNGRSLSIYLGDGLGGFTHSFAGAGLSPSWRPYHLA
                     VADFDGNGLSDIASANNDSNDILVFLATGAGAFGDPVRHATDRNNPTGIAVADFNGDF
                     KADLVVCYGSSQNAVNILNGNGDGTFQPHVNWTLDGNRNQYNMRVGDLNGDGYPDILA
                     PRWGALVARQNKATPVLSFNSASIWTELNQVLDAAVEDLNGDGMRDMIALSHDQAAVR
                     VYLGRGSFNLNADTIVAGMRHGYGRGHLETDSDSDYYQFSAKGGERLIIAAESPGAPS
                     SSGFDFNLYDHRGAHILGSSSIASNGYVYTQPFLIPYDGRYYLRVSPWHYSTHEYRFR
                     VTTVDAQTQIEAEVNNSTGQATLAAFNAASGSRSAKIFGHIAGNDTSGDFWSLGNLAG
                     GTQINVTLNRPQNSTFTTGLLRILNAGGSEVASSGASGLNVSHTVAGGAEGQYFLHFS
                     ESAGNRNLHCIYQAALSLTDALPPQITGTSLPAEGASTLAFINSFTLSFNEDMLASTV
                     TNPATYALREAGADMVLGTSDDVIYPVSPAAYSSGLSASFNLAAPLPPGIYRLTVAGL
                     KDTYGNNMPGAFVRNFSVAQVPGFTTINPGSNTPATATALVQVEEPDGLITGAGRGRR
                     TSSGDTHYWSFQGAAGQKLTFDTELVGASPSTDLYWRIRRPDNSVLWEGNIGNNNQGL
                     FATLTLNATGTHYVQVNEWNTWQEEYRFRLSLIDPALAQVEIESNNNIASATPLTLAP
                     MGDISVARAAGIVHTPSDLDYYALGAVPAGKTIFVSATLPATSSFLPVVAVYNSSGVF
                     MSEINGAPGDGSAEVRINTPGDYFMLVRSTGATGNYMSQYVAELQVHDTSSVAIPNLQ
                     VTEVVLPPEMGLQSGDTYTYEFTVTNVGSADIPDVPWSDRLVLSKNLIFGDQDDSEVG
                     VFVRNGGLGMGASYTVTGTSTLPQGAVGDYYLIARTDFTNQVDEVILENDNTSASATT
                     FPVALAPYPNLVVQNLGVTGPVADEFSITWNLANTGNLAAPAGFKERVRVVNTTTGAT
                     LLDELRTPGGISAGGSLARDASVVTTAAGVYQISVTADAQNDVYEHDGVSNTTAEVNT
                     AQTSFTILQYWTVSVSTPDPARGAVSGGGTFLAGTPVTVTATPDTSLLPWQFVRWTQN
                     GSFVSASAGYSFNATANRNLVAEFALPSYQVSASITPAGAGSVAGAGFFQHGSNAQLT
                     ATASQGYLFGRWEEAGATISTNAVLNLAVTGPRIFQAVFVEANPTHDVVTATNPPGLG
                     VTVGDGTFNNGQSSNFSASASVEAGDTEYLFDHWKLNGVFFGSQRVFSKTFSTLDAPV
                     MNFVAHYTSRSLKPVVTQVTSNYANPIRAAGNVQFTLTFDRTMRNNVEPVLVLSSANA
                     SETAVIPPGGSWISATQFRSADTTFGAGNGGAYVLNASAATDSNNRVMEPADVFSFDV
                     DVTPPPNPVLALSSFTGTSATVNWMGYAAPADLNAFRMYLQPADFASVAGIPAVSGLG
                     AAPRSYTFTGLQPDTQYYAAITAVDVAGNSDPAVTTLPVLMESTVPPPVNFLLTSPTP
                     ESARLNWNAYNTAALIGFQGWRVYMQETPFADVTGLTPVAQLAANVKTHDLAGLDRSK
                     TYHIAVVGYNRLDEFDPAVTTQAWTDPLAGQITANLTIGSAGADEVVEVLQSMIVRPG
                     ATLTVLPGTTLKFAPGTGLTIEGALVAEGTPLRPINFMSSTATPAKGDWNGVVLSNTN
                     ASTRLAHLWLRHGEGVQVVGGTPQISSLFCVQNEGQGIAASGNANVSVANSFFAFNDR
                     GVRASGNALLSVTGSVIKNNSVQGAEQSAPAVLDMQGNWWGSSAAGDIAAMIAGTVDT
                     GSPLAGEPVLAAGFATAGGITSTGVRNLAVVLASANATAFRLSENSLFPGVLWQEIFP
                     AGETNTFSPFPFPGSHLLSTGAGLKTVYAQFRSITGQTSAAIPVNVTLITTGPVINTF
                     SLADGQVITRPLAVTGSATAALGMNLIRLFANDTLLASAAGGSLNFQWDMRPLNAGPY
                     RMRLLARDNAGNESTREVNVTLDPQPPPAPVITAPLNNAVVATPAITVSGTAEPGVTV
                     RLTRNSAPQGSMTATVGGAFSFANVPLVEGANALIATAEDVVGTRASTAVNVELDSGP
                     PAPVVLQEPPIYDSFRGLILQWGFSPSGERPVKYRVFWHNAPFASSAAASGQSALLNV
                     QSYTLTSAPDGDLHISVVGYDAAGNASPLSNQIAYTLDRTAPVFTVSYDKAMPAGPGP
                     LTIVLESNEPLAATPLMTIKPNGLNTPISVPLTKTSGTTYTATYNVTNVSARTGAAVV
                     SVTGTDLTGNRFSGSPGGTALSFDVTPPAGALTFDRPVPIKTVSEADPAVAEDVSLSF
                     NLTLSEAPKPGTTPSLTFTPPIGADIVPALTGSGVNWTGSILLTASMGKGQGQFVFSA
                     TDARDNVGSSVTPNQLEIYNTEHPNAPGIPVNLAATTLSGGRIRITWNPVADAHSYRI
                     YRELGNTGVTPVVLVADGIEETEWTDETVPEDGIYRYAIRAFRVSIEGNPSGTLNALS
                     DRTPPNAPEDLDVTLTNSGLRITWNPPSDGLAPHRYHVYRNGVKIRTLNSAQPVLDFP
                     PKGLLQYEVAAADSYENESRAGPVPFEFFVDAVGSLLAQVNQGQPTVLSWTFNDPAGV
                     GYNVYRNGVKQNGQPIPVATKTFTDLLPLPPAESVEYRVTAVDNLSRESAPRTVRVLP
                     VTWSLVMNPDDEGAARVSRTRYFDNYRLTILNKDAATPLALNAVQVSRTLGGGAVTAF
                     TQPVSLEIAAASNESLDIPVPGPEFDAQSQNLEISVRAAPNEGGSLVAYTGSASNILA
                     AVEPPLMLEMRVTEPPVAGGLAEVKTRIHNRGYATMEVVLGRQTGQQPGDLFIQVLDE
                     SGAEISRRNFNGFGAPGLLIRPNGDAYVSIAPGASVEVSVEGVLIPIVLAETGRANFR
                     ATLSQVFAGLGTASQRVSGPLTGTLQSSVIETPYFGSVSTEKDIFAGDEPIVISGQAI
                     DRASSNPQGNVPMRIGFAARGAVWYQDVEADGDGNFSYSYQPFSGFAGELTIWAAHPD
                     VFDVLAQKTVRIYRIYATPSFGNIRMSKNDHLDFTIQLINPAELPLSGVGVNFRAYRL
                     DVDGVTEIPIPEVTCDFQAAGPPAGLAANKRTPVNLRLAAAVDAPDNANIEITFTTAE
                     GASTRFAGTLQLLPAVPILSMTSPRQGYVDVTVNKGQIVSQQVTLQNLGTRALLDVEL
                     VPPQLIPWMQINLPVNAQGNAELPDLDVGATVTFTVAFVPPENTPQGYVQDVVKIRGS
                     NAMAGFDFNLYALVSSEAKGGATFHVENFLGQEVPNATVRIRNSISGQEVGPLSTNAS
                     GDATFADLTEGLYSWQVTASGHNAATGTMEVVAAQTIIVEPVLQRSLVTVTFQVRPVP
                     FTDRYEIVIEQTFETFVPAPVLVMTPPKYDFNNVRPGFETTVIYELKNHGLIKIFNVD
                     ITGTYAGIMTLEPLIDFIPELLPQQVVQIPCRIRLGQNLAVAEAGGCDESLEDWIERR
                     SNPPPQINPDGYVFGGPAPTIGNFIGGFCPTPNLADFINGLSAIASLGANGCYASQGS
                     GNLGDALAVALAAAVVYETLTSIPGSVADVVMQVAAALGQCAAAHFGGGGGGGGGGGG
                     GPSRNTGNYGVGGNGCFTADTLVLLADGRQTPISKIKPGDELAIGMDSRETAKVSQLY
                     TLESDELLLMSLRSKDQPGNESRDLRVTGEHLVWRDGSGWTHARDLRPGDWLHDRAGG
                     MIEITSVKSVPGKHKVFTLQLKGGSTFYAGGVLVQDLCGGLYLDPSVLSKLEPVKE"
     gene            55931..59911
                     /locus_tag="PLP58_00645"
     CDS             55931..59911
                     /locus_tag="PLP58_00645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ07058.1"
                     /translation="MKTFLSIFPGPAAAVLLALMCDSRALATTQFQGWCSKVKIQIVQ
                     ELALERTGFEAELTVTNNDGADAITDFLAELTFTDPAITEPGANPDAANLFFVRQPTL
                     ENINRVDGTGVIAPTKTAVVKWFIIPTIGAGGTNPQGRRFTVGCKLSGKLQGVEIPRD
                     NMFAVPDTITVKPEPQLRITYFQPRDVLGDDPFTVDVVESPIPFTLGVLVHNHGFGPA
                     RNLRINSQQPKIVENLNGLLLVARLLGARVNDSPLDESSLLVDFGTLEPTQTGKGAWD
                     MITSLNGTFTDFRATYTHASELGGQATSIITALDAHFIAREVLNDDPGRDIIKDFLAD
                     VNRDADEIPDALYETQEGNILPVNHQQNAAVTGTLGANNVRVEIVSAFENWIYIRVPD
                     PGQNRKPISRVVRSDGKVLNANNYWTHTKYTRIGNVRLDWLNIFDKVADNTAYTYSLT
                     YDLDSVDVTAPVTRIRFSGEHTESGGIHHITRDTQIFFTSEDASPVAIEYRINAGNFI
                     PALPFTINASGTYTITFRATDTAGNVEEDKVALVSISGAGPSISSYAVNVGNLFLPGS
                     PNVVSVRPGQSELEFTVAPNPVRTDGRVDIFRGVAAWPRISGVPPTPTPLNAAELNVS
                     GKFVDFYRYKINEGSWSPERPVATPISLSGLNGSVTVSVIARSTYGDYLPEDDALEVA
                     WTVNPGAPLLNVAGVPPTPTPDQNSATLAFSGGADLTDYRWNLNNTFYQAEQPIATPL
                     ELTGLDPGDQVLGIRGKRSGIWQELNAATFIHWTYDPAYGSDFASLPLVKTQDYEDIA
                     GQTLTNVWDGKNGAGVPQLPGVYTVRLKLTDTLGRVSYDSDLVFIEKLSSGETQLGDA
                     GSSRPDARGSWAVWQRQVDGVPAIRARNLVTDTPVDVTSSPLAQEKPRTDGRYAAWQG
                     RGENGVFDVFYADLDAAMPAPVAATSTPARDEINPVIHWPWMVYQTRPSPANSAPWQL
                     EARNLETNAVFFVDTTTQDQLAPSIHSGRVAWQDWRDVGPGEIYFADLETGEKRRITT
                     QPAGQYNPTIFGHTIVWQDNRHIQLELYKFDLRKGLEERLTDTAYNEVNPVLCGNWLT
                     YLEDSLGSETENLRMLDLDSGESVPLTQAEGRHSPGALGDGYAVWAEGPAGAQTVKAS
                     FLPSLQPVMRNHNTVVVTAELAARYPTAFALLAAWNEAANVESITRYESIAVPPVTET
                     AAWVADAATGDDFAITPGDFLWIRFDQARMLELGEADAAPVDLAAGKNVLSYSAFPVG
                     YSAYDFINAVGAANIGALRMLDPLAGVWRAVEIKEGAPVGSNFAIPRVTALLVDMKNA
                     VNDWQP"
     gene            59941..60738
                     /locus_tag="PLP58_00650"
     CDS             59941..60738
                     /locus_tag="PLP58_00650"
                     /inference="COORDINATES: protein motif:HMM:NF012790.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HRJ07059.1"
                     /translation="MKTFVILSLLASSALRAATPEQLDAMLQTGEKVTIIDLRPTDEY
                     QLSHIAGAINIPHQLVPGKRLPPLGRVVAYCDGLGSTYTAECVAALNAKPGIQAEGLE
                     GGYAAWKTFTNVTADTRRVTPDQPKTITYEELKATRGEGVVLVDARKPQTVAGKPRVN
                     LAAFRQQNLPKATSMTNPSARLRAIKGKAGEFSKAPSLFVVVDDDHQSALAAAERIRA
                     MGYQRVVVLAGGEDIIRRDGRPGLGRQGGAAPVTLDPELVPPPAQPK"
     gene            60765..64031
                     /locus_tag="PLP58_00655"
     CDS             60765..64031
                     /locus_tag="PLP58_00655"
                     /inference="COORDINATES: protein motif:HMM:NF039835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ07060.1"
                     /translation="MKQASHFFLVLLLGLLQGLTAARAQVAISGGQAGNVDAGQFTVF
                     WQVDDTGSRPGLDLFSDAAATASLNGELGVEFFPLEMNDVTVGQTPAQREARRTLQAL
                     TKSKGIMLAKVTGAQPGATYYARPRSFDAGTGLANEAASAPLIEITTPESTAFIVESR
                     QVLVSTGGCFAGSQGLVMRLVKDGAPYPLFAVVGDHGQTDKALFDVNRLLNAAGTTNE
                     NLAGAPSFELDLIGPAAPLGVFNLDLPFAGEFVVAAATEVDFTANFPGLAYFSMEGTS
                     PPLQGVPFTFNITARDSGGNVLTTYDGTVDLTTGNPGDLISGAGETAHFVNGVLAGHQ
                     VVPAVSGPVTFTATRPCGIETGSQVFNFSPPNLDITFSAPLYTVSQGAMQVILTLTRS
                     ETLPASVILHTNDGTSQTANPPFAAALAGTDYLDLDGAATQVNFAQDETSKMVTVTLH
                     PKTGTRIPNKRFTATLHPPVAEAALTGALTTAAIHILAEDTLAPKLTVSKPSATTTAL
                     SLPQPYTVMGSAGDARGIDRVEIVLNGEPATFATLAPAPKPTSVPFTAEILPREGPNV
                     LEVTAFDLSGNSTTVTRNFTFTRRYWLTLHRIVPEPLEATPELAGAFALTASNNANKS
                     TLSPRAGTPQLSMVVPGTALQITANPAAGHSFSHWTGLPAESVVLGNVARFTMPASDV
                     PDVTAVFIVNPFLGLGVKADFLGLLHPSDSTSISNSTVGFLRGTLTTAGGSMSGKIHM
                     DGVAQSFVALVFGNGNVTFKVGRDTFTGLEFAGRMLTMSYTPGVLHLQVTSGAEESEG
                     QAVPAIYSATAPVPGALLNRSAKDGQPINQGYFTFVLPSKAQEPPLPLNSYPQGAGYG
                     TLILSNTGLLKVAGTLADGTKLAASSALVAGDASPLFAQILTPGTTTKALGGSLGGTL
                     VFDPLNADGDVSGEDLLWLRPAVTETATLATQIYTHGWPDGIRVDATGALYFAALNVQ
                     TGLDLPAPAPDGNAMLSFIGGRLAETITKSNFNISGNIFTKIPSTDRTFSLTLTPKTG
                     FFSGKFTPNWPSASTTQPSFNGVLLQKGVKKGGHGHSISNAVGDADPESSAVELLRRP
                     "
BASE COUNT        11890 a        20991 c        19706 g        11507 t
ORIGIN      
        1 ggtggcggcc cgccgggagg aggtcgaggg ccgggaggcg gcggccccgg tgccgggcct
       61 ggaattggcc catcaggcgg ctcttctccg agtggagtga gaatccagtc agctccttaa
      121 actcatgcta aatttcgttc acaacccgca atcaccccgc tcctcggcaa gcgcgcctgc
      181 ggcacctggc aggcgctttc accagaatgc gggcgctttc gaatttcgaa tttcgaatcc
      241 ccctccttct cgccaaggct ccgccctcat gctcatggtt tgggcactgc tgctgatgtc
      301 tgtcaccgtc atgggcgtgg tggagtacat ccgctacagc gccgaggagg caaagctggg
      361 cgcggcggag ttttacgcgc ttcatttggc ggagagcggg ctggccgtgg ggctgcaccc
      421 cgaggcgcgc agaggcgacg ttgttttgaa gcaaaaaatc ggcgccgaca gcggcttcga
      481 tgtcgtcatc aactacgaag gcgcgcgcat tcccatcaac tacatcaccg acgaccgtct
      541 gcgcgaggcc gtctatcagc tcctcatccg ctggggcttg aacagcgacg aggccggagt
      601 cgccagcgag tccctcgccg actgggtgga ccgcgatgac gatgtgcgcg ccaacggcgc
      661 cgagtccgcc ttttaccagc agcagggcat caacgacatg ccgcgccagg ccggcttcat
      721 tgatgtggat gaaatgctgc tcgtgcgcgg aatgggagtg gtggaccgtc tcaagccgga
      781 ctggcgggaa tttttcagcg tgtatggaga cggcaccata gacttgagaa ccgccttcaa
      841 agacacgctc atcgctgtca caggagcctc cgaatccgat gtcaccaact atatctcccg
      901 ccgtgacggt gcggacggca tccccggcac cgaagacgac cagcgcatct ccgacagcga
      961 ggcctaccgc ctactggggc tctccggaga ccgaggccgc gcgctcagtt ccatcctaac
     1021 aagcgaggac tctgtgcgcc ggatcaccag cacgggatat gtcggggaaa agcgcgcgca
     1081 aatcatcgtc gtcgcccgca gaggcgagga ccgctccctc acttatctgg cccgcattga
     1141 ggaatgattt cctttgcccg caacttcgcc aaacggcaag cgttccaccc caacttcatg
     1201 tccatctccg cctcgatcct gctgctcccc gcccccgaca cgacctggcg tgtgtggaaa
     1261 ccccgcgcca gcagcgcgga cgcctccgtc gaggctccct ccgaggccgc cggcaaaggc
     1321 aaagccttcg tcatcggcct gcccgccacc gcctgccgca ccatcgggct tgtgctgccc
     1381 aatgcggacc acgaggtact ggagcagatc atcatcaccc agctcgaaag acgcggactc
     1441 aaactccacg cggtgacgcc gcgcaacttc cgctggcatc tgctcaccca gaccgcatcc
     1501 accgcaaccg tcagtgtgga tgtgctggca gatcccttcc ctgaaagtct cgcgctgacc
     1561 gacgccaccg attacaccgc cgcattacgc atggtgaacc tgcccgccgg gcaaatcatc
     1621 gtcaccgagg agcacggctc gctcgtgctc gtggcgggat ttcagggcaa gctctaccac
     1681 agccatctct ttgccccggc ctccgctgcc gctgaggaga tcgcccagga gatcgtgcta
     1741 gcccggctcg ccctggagca ggatctcggc gcgggcagtt tcagcggcat caccctcgcc
     1801 ggagcatcct gggatgccaa tgccacccgc cctctgcagg aactcaccgg actgcccgtg
     1861 cgcatcgtcg cccagcttcc gccgaactcc gatctcgaca ccagccactg gacacgcctt
     1921 ctgcccgccg gcgtccatgc cgcgcagagc gcccgcgcca gagccgccaa gttgctccgc
     1981 ttcggcgtgc tcgccggcat gatggtcgtg gccctcggct tcttcgcctt cgcctacctg
     2041 cgccacctgg acggcaccgc cgcagccctg gaaaaagacg tggccgccac ttcggaagcg
     2101 gcaggcaagg tcaaagccac cgccacgcat tggaaggccc tttcccccgc catcgagccg
     2161 cgccgctacc ccctcgtgct gctcgccgag atcaccaagc tcatgccacc cagcggcatc
     2221 gtcatccgcg attgcgacat ccgcaccacc gagatagaca tccgcggcga ggcgcgcgac
     2281 gcgcagttgg cattccaatt cgtcgaagac ctccaaaagc accccgtgct cggcctttac
     2341 acctggtcca agcctcaacc caccgtgcgc gaaaagaccg cccaattccg cgcccagggc
     2401 aaaatgcagt gagcggcccg ccggtctcca attccaaatt ccgtcaatcc atgcagcaaa
     2461 aactcaccgc gcgcgaaaaa aacctccttc tgctttgcgt gggagtgctg gtattcatgg
     2521 cgctggccat caccgccaac ggctttatgc agaagcgctc cgccttgctg caaaaaatcg
     2581 cggcgatcga agtccaaaag cgtgaaaacg acacctggat ggccgaccgc gccttctggg
     2641 aaaagcgcag atcatggctg gaggaaaaaa tgccctccac cgacagcctt ggccgcgccc
     2701 agggccagct cctggagcag ttgcagaacg aggccctcga tctcggcatc gccatccagc
     2761 agcagaccct gcccgaagcc gtcaccaccc ccgactacag ggaagtcact gtgaacctgc
     2821 gcgtgcgcgg cgaccagacc gccgtgcttg gctggctggc caccatgcaa tccccggaga
     2881 aatttcaagc gatcaagcaa ctggacatgg aactcgactc ccgtgcccgt gagacaaccc
     2941 cgcagaccct ctgcaacctc accctcgctc gctggttcaa acccgaaacc ggcctttaaa
     3001 ccctgatctc ctccacgcca tgaaaaaccg cctttttatt tccctcgcct cgctgcccat
     3061 tctcgtcgcc gctcagaatg ccgacccgga cctgccgcaa gcatttgatc ccaaaagcgt
     3121 cgcccccgtt ctggagagtt cccctttctc ccgcatggtg aacctctctg acaatctggt
     3181 gctgacgggg atcgcctatg tggacggcaa accggtggcc accattttta acaaagagac
     3241 caaacagtcc agcgtggtcg gcagcgagcc gaattttcag ggctggatac ttcaggaggc
     3301 cctgcccgcg ccggatgtta agctcagtca ggccaaaatc agcatcggtg gagaagtcgt
     3361 cagcgttcgg tatgccactg tcagcgccga ggaaatgcgc ggcgagcgcc gccgggatgg
     3421 cgagcgcggc ggcccacccc ctcccggagg tgagggtgac cgcttccgcg gccccagccg
     3481 cggacccagc gaggaggaca gacgccgata cgaagctctc tccgagaagg gaaaggaaaa
     3541 gcttcgggac atgatgcgct ccaagttcag cgatgaaaaa ttccgcaacg cccccgaaga
     3601 agagcgccgc aacatcatcc gcagagaagt cgagcggatc gaaaaagagg accaaggcgg
     3661 acgctgagag ttttcccgcc tctccgctgc aaccactctt cccgaccatt ttccagcctc
     3721 atgcattctc ataaactccg ccattgtctt tccgccagtc tagccctcgt atgcgcgcaa
     3781 ctcaccgccc aggacaaccc aaccagcgct caggccgcag cctctcaagc acctgcggct
     3841 tcaccggttc cagcgggtcc acaggtgcct gctgacccgg ctgcccagca agccccgccg
     3901 gcgggagatc aacccagtgg cccgccaaca ggagcccctc ctggaggttt cggtcccggc
     3961 ggcttccgtg gcagacgcgg cgagggcggt ttcggaggcg ctcctggggg cggcggcgga
     4021 gcaggcggcg gcatcgccag caccctgagc gagaccatcc gcatggatgg cgaccagatc
     4081 gtgctgcaat tccccaacaa ccccgtcgcc gacatgctca gcatctacga gttgctgacc
     4141 aacatcaccc tcatcaagga caccaccatt tttgaaggtc cccaggtcag cctggccacc
     4201 ccgaagcccg tctccaaaga ggaggccgtc aaactgatcg aggccacgct gttcaccaac
     4261 ggctacgcca ttgtcatgga gcctgatggc aggagcgccc gcatcctgcc ggcgcgcacc
     4321 cagagcgcca acagcgttca gttctcccag ggcgtgaatt tttacacctc gccactcgac
     4381 ctgcccgaag gggaaacgct cgtgagttat ttcatgcgcc tgaatcatct cgaccccgaa
     4441 gaggcggcgc gcatgctgtc aggccacgtt ggactcggca tttacgggcg catcaccccc
     4501 gtgaccacac cgccgggcct tctcatcact gaaaacagct ccattgtctg gcagcttatc
     4561 aacatcaagg aggccataga catcgccgac acaggctcgg ccctgctcac caagttcatc
     4621 cccgtcgagt acgccgacgc tgccacagtg gcccagatca tccaagccac actcgatgcc
     4681 cagctccagg aggaggagac ccgcggcacg aacactttcc gcggcagccc ggtacaggac
     4741 aatcgaagca atcagggcgg aggtgaccag cgttcctcca gtccgcaacc ctcctcgcag
     4801 aatttccccg gttccagcaa tcgttcctcc tcacaggaca attcccgctc caccgctccg
     4861 aaacccaaag cccaggttgt tgccgatacc cgcctgaacc agatcctcgt ggtctcctcc
     4921 cccgacgact acacctacat cgccagcatg atcgccgagt tcgacaaggt gctccctgtt
     4981 gacgagcctt atgaacgcaa actcaaatac gcctccgctg tggacgtgct cagcgccatc
     5041 gccgacctcc tccaggacac cggcggcgga gccacgcagc tccctggagg cggcacactc
     5101 cagcagcagc ggaatcaagt gctgacttcc accagcagcc agctcctcac aggccgcacc
     5161 accaccggca ccaggggcgg ccagcttctc gccaccggcg gcgccgacga caccgcaacc
     5221 ggcggcaccg gcaccgtcag ccgcgccgac ctcatccagg gccccaccga ggacaacgcc
     5281 cccatctccg tgctcatcgg caaaacccgc gtcgtcgccg atcccatgtc aaactccatc
     5341 ctcgtcatgg gccgcaagga tgccgtggac aaggtcaacg gcctgctcga caagctcgac
     5401 cggaaacccg cccaggtcta tctctccacc gtcatcggcc agctcactct tggagacggt
     5461 tttgagttcg gcgtggacta cctctcagcc ctcaccagca aagaaggcac caacttctcc
     5521 ggcagcagct tcgtcagccg ccaggacgtg ctttccggca acgccgtcac aagagccgtc
     5581 agagacttgc gcgacaacgt catcaccacc cccttcggcc ccgccgccgg aatgaacctc
     5641 tacggcgcca ttggcgacag cctggaggtg ttcgtcaccg ccctggaggc caccaaccgc
     5701 ttccgcgttc tttcccgtcc gagcgtcttc gcgctcaaca acaaaaaagc caccatcacc
     5761 tcaggccagc tcatccccgt gcccgcgcag accatttccg tgcccggcaa caacaacaac
     5821 gtcaacggct ccgtcaccac caccgtcgaa taccgcgatg tcgtcctcaa gctggaagtc
     5881 gtccccctca tcaactccga cggcgaggtc cagctcacca tcgcccaggt caacgacacc
     5941 gtcatcggca cccagcgcgt cgagcccaac gacatcccca tcatcggcac cgagcaaatc
     6001 atcacctccg tcaccgtccc cgaccgcaac accatcgtcc tcggcggcct catctccgag
     6061 caggaaaagc gcgacaccca gggcatgccg ttcctcagcc gcatcccggg cgtgggcaac
     6121 cttttcaagg acaacaaaga cagcaccaac cgcagcgagc tcatcatctt catccagccc
     6181 caggtcatca acgacaacaa cgccctccgt ttcgcttcaa tgcgtgaaga cgtgcgcacc
     6241 aaagtgggcg ctgacgcggc ggaaaaattc ccccagcagc ccgtcatccc gacccccacc
     6301 accgctcccg gcgacactcc tcagaaaaaa ggcatcttct cccgcatctt cagcagcaag
     6361 cgcgccagca cccccctgcc cggcaggcaa cgccagtgac cgcgccctgg cgctgaaacc
     6421 aaagacgcgc ccgcttgatc tcaagggatc tgacatccct gcgccgcctc ccaaagcgca
     6481 taccaatcct cgcgctccag ctcgatgccc gccgccctcg cggtcgtcgc gaggcgctcc
     6541 agcttgttag tgccaatgat gggcagcggc tttgacgggt gcgccatgac ccaggcataa
     6601 gccagttgat ccaccgtggc atggccatat ttggccgaga gttccgcaca cctggcgtga
     6661 atgcgcgccg cagccggatc cgccttgtcc atcagccggc cctggcccag cggcgaccac
     6721 gccatgggac gcacattcaa gcgctggcat tgatccgccg tgccgtcata aatgggcgcc
     6781 atgtgcagca ggctgaactc gatctggttt gtcaccaggg gccgctccat gcgcgagttc
     6841 aggagttcga actggtgcgt gttgtagttc gacaccccgg catgaaggat tttcccctcc
     6901 cgcaacagcc ggttcagccc ttcggcggtt tcgtcggcgg atgtgagcca gtcgggtcgg
     6961 tgaacgagga aaaggtcaat acggtcggtg ccaagcaggc gcagggattt ctcagcgctt
     7021 ttgacgaggc gctccgctgt ggcgttgtag tacgcggtct tgcgctgcgg atgaaattcg
     7081 cacggcacat agatgccggc cttggtgacg atctcaaatt tgtcgcgcag agagggatcg
     7141 gcttgcaatg cctgcccgat gaattcctcg actttgtaga gtccgtagat ttcggcggtg
     7201 tcgagagtgg tgatgccaag atcaaggcag gcgcgcaagc gccggaggag gtcctcccgg
     7261 ttcgccgtgg ccgggtcgtc gaggatgcgc catgtgccgt agataaggcg ggaaaattca
     7321 gggccgttcg gagaaagctt gagtcgcggt gtcatgatcg ggtggaaaaa tcaggcggcc
     7381 atgcaaggcg ggaaaagcgg cgcggcaacc cgcaaaaaaa cccgcctgcg gaactgcggg
     7441 cgggttgttt cgaaaagttg tccggcggat caggcattct ttgcgagcga gccgatcttt
     7501 tgggcaaggc ggcttttcag tctggatgag gtgtttttgt gcagcacgtt ggtcttggcg
     7561 gccttgtcgg cggcggaggc gaattcaccc aggcttgcat tggctgccgc cgtgtctcca
     7621 gactcgatcg ctgcgagcac ttttttgcgc agggtgcgga cgcggctttt cacggcctga
     7681 ttgtgtgccg tgcgcttgac ggttttgcgg atgtcttttt cgctggagcg gatgtttgcc
     7741 atggtgatac gaaaggggcg ctgtgtataa gtcagcgcat cagggctgtc aaactggaat
     7801 cttccgccca tggaaggggc cgaacgcaag cctcgaatgc acgctgcctg ggtattttaa
     7861 gctggagcgg gaacgctgtc ttgcccggtc cgcagatgct ggcatggtcc gtcatgccgg
     7921 acatcgtgct cgccacgctc aacgccaagt acatccactc atccttcggc ctgcgctgtc
     7981 tgatggcgaa tcttggcggg ctgcgagagc gggcggtgct gcgcgagttt gtcattcaac
     8041 agcagccgct ggagattgtg gaggcggtgc tggcggaaga tccaaagatt gttggattcg
     8101 gagtctatat ttggaatgtg acgcagacga ccgctgtggt ggctttgctc aaacgcctgc
     8161 ggccggactt ggtggtgatc gtcggagggc cggaggtctc gcacgagacg gaaagccagg
     8221 agatcacggc gctggcgaat catgtgatta caggagaggc ggatattaag ttcgccgaag
     8281 tttgccgggc gctgctggac gggacggacg cggaggcgct gccgcgcgtc atgcctgcgg
     8341 agctgcctcc gctcgcggca ttggcctcgc cctacgacct ctacacagac gaggatctga
     8401 cccaccgtgt catttatgtg gaagcatcac gagggtgccc gttcacttgc gaattctgcc
     8461 tctcgtccct ggacatcccc gtgcgcgcct ttccactgga gtcctttctt ttctccatgc
     8521 agggactcct ggatcggggg gccgcgcagt ttaaatttgt ggatcgcacc ttcaacctgc
     8581 atctgcccgc gagcacggcg atcctgcgct tctttctgga gcgctggagg gagggacttt
     8641 tcctgcattt cgagatggtg ccggacaggc tgccagtgca gcttcgcgaa ttgataagcc
     8701 ggtttcccaa aggcgcggtg cagttcgagg tgggcatcca gacctttgat gagaccgtgt
     8761 caaaaaacat cagtcgcagg cagaacctgg agcggctgga ggacaatttc cgttttttgc
     8821 ggcaggagac gggcgtccac attcatgccg acctcatcgc gggtctgcca ggagagagcg
     8881 tggagagttt tggcctggga ttcgaccgcc tggtgcggct ggagccgcag gaaatccagg
     8941 ccggcatcct caagcgcctg cgcggcacgc cgatcacgcg gcacgacgct gaataccgca
     9001 tggtttactc gccgcatccg ccctatgaaa ttctgtccac gcgcgacatt tcgttcgagg
     9061 aaatgcagac gatgcgccgc ttcgcccgct attgggactt ggtggcgaac agcggacagt
     9121 tcaccggcac tttgcagctt ctttggcgcg gcgcggcatc cccttttcaa aggttcatgc
     9181 gattcagcgg ctggcttcat gcaaggctcg gccgcactca tcagatcgcc ctgcatgtgc
     9241 tggctcggga attgcacgag tttctgactg gtgaactcgg cgtggatcgc gacctggccg
     9301 ccgaggcgct ggagacagac tggagccgcg cgccgggacg cggcgcgctc aatctgcgcg
     9361 gcggctccat gacggaaatc aaacccgcct ccggcaggat cgcccgcagg cagtccaggc
     9421 acatgcggga atgaagtcca ggcttcccgc gccagaggcg ccgtacatcg ccgaaccact
     9481 ggcacacgcg gaccaacccg tgcatgatga gcgcgtgcca atttatctac acgagctggg
     9541 tttcgacgaa ctgcgcgcgc ggctcgctgc cgacgggctg cgccctgccc atgctgccac
     9601 gctttggaac gccctccaat accgcgcgca gaccgatctg ttggcacggg aggacttccc
     9661 tccccctctg cggcgctggc tggagcggcg gctcggcgtc caatgcctgc tcgacatgcc
     9721 ggaggtttcc agccacacac ccagttcaga cggcctgacg gacaagttcc tgctgcgcct
     9781 tcaggacggc caggaaatcg agaccgtcgt catgggctac gagggacggc acaccgcttg
     9841 tgtgagcact caggcggggt gcgccatggg ttgtgttttt tgcgcgacag gccagatggg
     9901 tttcgcgcgt catttgcgcc ccggtgagat tgtcgcccag gtgctgcatg cgcggcgggt
     9961 gcttcagggc gcgggcaggc ggctgcgcaa tcttgtgctc atgggcatgg gcgaacccct
    10021 gcacaactat gacaatgtca tgaaggcgct gcaaattctc agcgacaccc ggggctgcaa
    10081 catcggcccc agcaaaatga gcatcagcac cgtcggtgtc gtgcctggaa tcctgcgcct
    10141 ggcggaggag gatcggccct acaatctggc cgtgagcctg cacggcagca ccgaggagga
    10201 gcgcgccgcc ctcatcccgg ccaatcagcg atggccgctg catgagctgg tccaatcctg
    10261 ccgcacctat ggcgagaaga ccgggagaaa aatcttcttc gcctggacgc tcattgccgg
    10321 agtcaatgac agccccgctc acgcgcgtcg cgtggccgcg cttttgcggg gcttgcgcgc
    10381 gcatgtgaac ctgataccgc tcaatgaaac tgccggctac gcggggcgtg aatgcgcgga
    10441 gtcctccgct tctgtctttc acaggatcat tcaggatgcc ggcatcccct gcaccatccg
    10501 ccaaaaacgc ggaatagacg tggccgcagg ctgcgggcag ctcaaagcgg caaggcgcaa
    10561 acgcgagcgt gccgctccac aagccgcctg actggcagct cgcaaaacat gggccttttt
    10621 cgcgcgcttg ccttttcctc cggttccaag tggcgtgagt tcactcaaag atgacctggg
    10681 caatcgccag ttcgcgggtg tggctgaggg aaagatgagc ggcgcgaacg ccgagggcgt
    10741 cggcacgggc taaagcggct ccatgcagca gcaagcccgg cctgccattt gcggcgcggg
    10801 tgacctcgaa gtctttccag tccaccccct cggcccagac gccggtgccc agggccttgg
    10861 cggcggcctc cttggcggcg aagcgcgcgg cgaagtgcat ggcgggatcg gcgcatgact
    10921 cgcaataggc gcgctcgcca tcggtgaagg tgcgcccgac gaagcgctcc gcgtgctttt
    10981 ccaaaatgga ccggatgcgc ggcacttcga cgaggtcaat gcccgcgcct ttgatcgaga
    11041 cgcatgagtc ttcgagtttc acaaggcgta ggtgttcatc acggagcgga attccgccac
    11101 ggcctgcctc aggcctgtga agaccgcgcg gcagacgagg ctgtggccga tgttcaactc
    11161 atgaagatgg ggcacggtga aaagctccgg caggttgcgc gtggtgagtc cgtggcccgc
    11221 gttgacccgc agcccgaggc tgtgcgcaag cagcgcggcg gctcgcaaac gatccgtctc
    11281 agccgcgcgc gcctcacccg tttcattggc gaaggtgccg gtgtgcagtt cgaccatgct
    11341 ggcgccgctc tccgccgagg cgcgcacctg ctcctcgtca ggatcaatga acatgctgac
    11401 gcgggtgccg ttgttttcaa gctggcgcac cgtaccgcgc agggcttcca tctgcccggc
    11461 cacgtcaagt ccgccctcgg tggtgacctc ctcgcggttc tccggcacca tgcacacaaa
    11521 gtcaggcttc acgcgcaggg cgatttcgag gatttccgcc gtgttgccca tttccaggtt
    11581 cagcttggtg ccgatctttt cacgcagatg aaagacatcc gcgtcctgaa tgtgacggcg
    11641 gtcggcgcgc agatggatgg tgatcgaatc cgccccggcg gcctcgcatt ccagcgccgc
    11701 gtccaggatg catggctcga cgttgtgcgc gtccggcatg gtccggtagc gcgcctgccg
    11761 cagcgtggcg acgtggtcaa tattgacacc aagatggagc ggcatggcgg ttaatgcagg
    11821 aaatgacggt ggccggtgaa gatcatggcc attttgcgct cgtcggcggc ggcgatgacc
    11881 tcctcgtcgc gcacgctgcc gccgggctgg atggcggctg tggccccggc ttcggcgcag
    11941 gcaatgaggc cgtcggcgaa ggggaacatg gcgtcgctgg cgacgacgct gccatccagg
    12001 ctgagaccgg cttctttggc cttccaaatg gcgatgcggc aggagtccac gcggctcatc
    12061 tgcccagcgc cgacgccgag agtgcggtca tggctggcga agacgatggc attcgacttc
    12121 acatgcttga ccacccgcca ggcgaaccgc atggcctcga tctcctcgcg ggtgggcggg
    12181 cgctgtgttt tgactttgtt ttccaaatcc tcaaggccaa gcgcgcgcgg atcactgtcc
    12241 atgaccatca caccccccgg cgcgttgcgg atgacaggct cgcgcaaggc ctcgaccacg
    12301 gaggggtgca tctgaatgag gcggaggttt ttctttttct ggagatgggc gcgggcgtcg
    12361 gcgtcgaaat cgggggctat gatgacatcg gtgaagattt cggaaatgat gcgcgccagg
    12421 gagagcggca tggagcggtt gatgacgatg acaccgccaa agggcgcctg cttgtcggtt
    12481 tcgaaggcct tggtccatgc ctcgcgcagg tcctcctcgg cggagccgac accgcaggga
    12541 ttggtgtgct tgaggatggc gagggtgggg cggcggaact cgtggatcaa ggcggcggcg
    12601 ccctggatgt cgagcacgtt ggtgtagctg agatccttgc cgtggagctt ggtgaagtag
    12661 tcgccgaagt tgccgtaaag cgcggcggct tggtgggggt tgtcaccata gcgaagctca
    12721 ctgtagagcg gcaggctcag ggagaaggtt ttttgcgtgg tctgctcctt gttcagatag
    12781 gccgcgatgg cggcatcata ggcggcggtg cgctggaaaa ccttgcatgc gagccgctcg
    12841 cgtgtggcca ttttggtccc gccctcgttt tccttcatct caccgagcac gcccgcgtaa
    12901 tcagagggat cacagacgac ggtgacccag cggtggtttt tggcggcgct gcgcagcatc
    12961 gaggggccgc cgatgtctat gttttcgatg gcttcctcaa ggctgacatc tggcctggcc
    13021 accgtctgct cgaaggggta aagattgacg accacgagat caatgaccgg gatgctgtgt
    13081 ttggcggcgt tgcgcttgtc ctcgtcgttg tcgcggcgtt ggaggagtcc gccgtggact
    13141 ttggggtgca gggtcttgac gcggccttcg aacatctccg ggaagccggt gtattcagag
    13201 acgtccacca cgctcagtcc ggcgtccttg aggtgtttgg cggtgccacc cgtggatagc
    13261 agttcgacgc cgagcgcggc gaggcctttg gcaaagtcga gcaggccgga tttgtctgag
    13321 acggagagaa gagcgcgggc gatgggcatg ggcggggttg cagtgactgg atgttgtggt
    13381 tgatgatggc ggctggtcct ggtttttcga atttcgatac ttgtgtatct cactccacgc
    13441 tgacggtgaa acggcgggtg ggttgtttgg caagttcgag gacgacgaga gcgtccactg
    13501 cgtcagaggg tttgcgggtg ggtgagagat cgaaaaccgc accgccatcc ccgccgttga
    13561 ggcggagacg gaggttgatg ccgagctggc tgttgccgtt ttccagcgcc cactcgtgaa
    13621 gatacaaata agtgcgcggc accgtcaggc gaatcagacg cctgctttcc ttgagggtga
    13681 gaagctccgc cttgaattcg gcgggatcaa aaccagccgc gtatccggtg ccaaaagccc
    13741 aggctggcac atggtccact tttccagggc cgtccacagc cggtccgctg acgcgcaagg
    13801 ctgcggcagc cccggattcg agcctcttgc catagctgcg cgcgtccagt cgcagcagca
    13861 attcccaggc gggtccggcg ggagagtcgg tgagcaagcc tgagtcggcg agatcgagca
    13921 tgagcgtcag cgtggatgca tcggcatgag cggtgagggc ggctttgcca tccagcgcag
    13981 ggtcggatgc ggcaagcggg cgagcggtgc cgagaccgag gttgggcgtg aggcggaggg
    14041 agcgcgtgga ttcatgtttg aagtcgccct ccagcctcaa agagagcggc aaggcgcggc
    14101 ctgatgcgtc actcggaagt tcgaaacgta tatccagagt ctggctggca ccggcgggca
    14161 cgtcatactg ccccttcaga gtcgctccgt taaacccggc ctgccatgag cctttaagcg
    14221 gtcgtttgcc atggttcacc aggacgcacg cgggcgaaat ggcgctttcc tgattgaaaa
    14281 aagtctccat ttcccagacc accgcagctg gctgcagacg gcagcgcagt ccggcaatct
    14341 ccaaacttcc gcctccgctc agcagcacgg gcagacgcac cagcggctca tcaacagcgg
    14401 caggctgcgc gcgcgcgaaa gtagcctcca actccgtgct ttccccgggc ttgatccgca
    14461 cggctggcac cgcctccacc ggacgccatc cacccgcgat caaaggcagc gcgatgagat
    14521 cgagcggctc tttcgtggtg ttttcgagca tccaggacac ccgcagtccc ctgccctggg
    14581 catccagttc agcctcgcat ttgcccatgc tccagggcac tgcgggcgga ccatcaagga
    14641 gttgatccgc caaagccgct ccaagacgcc cgtgcagtga tgcgcggggg atgaaacccg
    14701 ccccgctgtc atgaaaaaag ccgcgataga cccgctccac ttccgcaaac caatgcgcca
    14761 catcctgacc ggcctccagc ggcggagcat ccatgatccc gcgcaggccg ccaagatcca
    14821 caaaaggcag cccatgctcc acggcgatct ccgccagcac gtcagcggca ggccgcgcca
    14881 agcccagaac atgctcggag gcggcgctca tcggcggcca tgatgaggcg agaatgacct
    14941 ccgcccccat ctcccgcgcg gcactgacgg cctccctcac ggctctggag aaaacgaccg
    15001 gagccatgcc cgcctccgcc tggccgagac agagcagcac cagattcacc ggtgtctgcc
    15061 gcgcggtcga agccaggata cccggcgcgt cgagcaccga gcctccgggc ctggacagat
    15121 ttcgaagcac caaggcaggc cccaactcca cgccgggctg gtctttttcg gcgatacgca
    15181 ctccgccggt gtaaaaaaac agcgccgcca agtcatgcgc gaacacttca tcaaaggccg
    15241 gcgcacctcc gggcgagtcg ccttcctccg gccacacagg tgcggcatcg cccagcatga
    15301 gcaggtggaa aggctcccgc tgcaggagcc gccgtgtgga ttccggaagc agttgccgca
    15361 gccgcgcctg gcgcgctgcc gccatgtcgc ccgtgcccgc gatggccgcc tcgggcttgg
    15421 gaagacgctg ctccaactcg cgcgccgcct gccgccgttc ctcaagggag gcctgggcgc
    15481 ccgcattcac agcggccagg gccgtcagcc cggccagccc gctaatgcgc gcgattttcc
    15541 tgaaagagtg tgtcatcgga cgtggcactt catccggttt gccgggcatt tccagcgcaa
    15601 aaaacagccg ctccagtcca cccacggcgg ctttgacaat tgccatccat tcttcattcg
    15661 tggctgacca ccttcccatc cgccgcttca atgccagacg ccagcgccac caccctgcac
    15721 cccgccctgc ccggaaccct cgtgatcatg ggggtgtgcg gctgcggcaa gtccctcatt
    15781 ggcgcaaagc tggcggagaa acttggcggc gtgttcgagg atgcggatga tttccacccg
    15841 ccggagaaca aggcgaaaat gaacgcgggc atccccctga ctgatgagga caggtggccc
    15901 tggtttgacg ctctgcgctc gcacattgag gacatgcgcg gacgaacgac ggtttttgtg
    15961 cttgcctgct cggcgttgaa ggccgcttac cgcgagcggc tgcgcggggg cgatgacagc
    16021 gggggactgg ttttcatcca tctcaaaggg ccgcgcgggg tcatcttcga gaggatgtcg
    16081 gcgcgtcagg ggcactacat gccggtctcg ctgctcgaca gccagcttgc cattttggag
    16141 gagactcccg atctatggac agtgagcctg gagcaggagc caggtgaggt ggtggatgaa
    16201 atcctgcgcc gcctccgcga gcccggaatc gcgggggcat gagcgcgcca gtctggcatc
    16261 gcagtctggc ggcgcggcac gcgctggcga tgcttgcctg cgtggcggga ggctcgatcc
    16321 tgctgctggc ctggctgggc cgcatgcaac acacggagtc cgccagcgag gccgccgctc
    16381 tggcaaaaaa ccatgcggac ttcgtgcgca ggctgaacct gccgcgcagc gcgcgcctgg
    16441 cgggtgattt gcgcgagctt ctcggactgg acatttattt tcgttcaatg actggcgcac
    16501 tcgatcccat gccgggcggg gatttgaaac ccgccctcat ggaggcgcgc acgggaacgg
    16561 actggcagcg gcttccggat ggcaggcagg ctcttgcgct gagcctggat gaaactcatg
    16621 acatgatctt cgtgcgcgcc gagccgccgc tggcgctgag cctgcggcat cccgtcatgc
    16681 gccacgcgct gcccgctttc tggctgctgg ccgcgctcat ggcatggctc nnnnnnnnnn
    16741 agagtcacaa agccgcgctc cgccaactcg gccgcatatt gccggttcgg cgcgcgccag
    16801 gtggtgcggc cattgaccgg actgacacga atgctgcccg gttttttcag caaggacggc
    16861 cccatgccgg cggaggcggc gaggcaggat gagatcggcc tcctcgcgcg cgctctctcc
    16921 cgggcgcgcg ccgacttgca tcaggaaagg cgccggcgcg aggaatccga gcgcctggcc
    16981 ttgctgggac gcgtggccac cgggctggcc catgaaatca aaaacccgct ggcttccatc
    17041 cagcttcacg cgcaactgct cgacagcgca tctcacaacg ccgagaccgc ctcctcgctc
    17101 aaacatgtga tcgaggaggc gcgcgtcatc gagggactgg tgaaccagtg gctctttctc
    17161 gcgcggccct cgccaccgga aaaggcgcct ttggaactgc gcggactttt gtgcgccact
    17221 ctcgactccg tgcgcctcca agcgcggcac gccggcgtgc gcatgaatct cgaacccggc
    17281 ccggaggtca ctgtcccggg cgaccgcgtc cggctcggcc aggccttccg caatgttatc
    17341 ctcaacgcca tccaggccat gcccggcggc ggtctgctca gtgtgagact gcaaagtgag
    17401 ggaacccatg cgcgcctggt cttccatgac agtggccccg gattcagccg ggccgcgctc
    17461 cagcaggcga ccggtctttt ttactctgaa aaagagggcg gcatgggagt gggactcaat
    17521 gtggtggagg agattgtttc cgcccatcaa ggcagcctgc ggatcaaaaa tcatcccaaa
    17581 ggcggggcgg tggtggaatt ggagttgcca tgcggggctg acggggattc ttgagcgtta
    17641 tgccacgagt tttgatcatc gaggacgacc acgcgctcgc cgccgcgctg gccgtggcgg
    17701 cccgccgtct gggcgccgag cctgcagtgt cagcctctgg tcagggcggt ctggaaaagg
    17761 ccgggcgcgc ggccttcgac gccgtgcttc tcgacatcgg cctgccggac atgagcggac
    17821 tgagggtgct ggagactctc aaagcgcgcg gctgctccgc gcccgtgctg gtcatcaccg
    17881 cgcatggcac gctggatcat gccctggagg cgcgccgcct cggcgcgcat gaatattttc
    17941 tcaagccgct caacctggct gacctgcaaa gctgcctgcg cgccctgctc aagccggaca
    18001 aacctcagca gccggcgccc gccgcgccgc gtccgctgat gatcggctcg gcgcccgcca
    18061 tgcagcgcgc cttcgccgtt gtcgcccagg cctgcgcgac gcgcgagcct gtgctcatca
    18121 ccggcgcgcc gggcacgggc aaaaccctcg ccgcggaagt catcgcccgg cattccgcgg
    18181 acagctcgcc attgaaaata ttccgttgcg acgagtggcc agacggcaag gcgcgcgccg
    18241 cgttccacga ggctctggca ggcgaaggcc cgctcctgat cgaggaagcc gccagcctgc
    18301 cgctgcctct ccagggcgac ctctccgcag ccttgcagga aaacacgcgc cgcattctcg
    18361 ccaccacatc agccgatctg cccgtcaaaa tcgccgcagg cttgtttcgc gaggatctgc
    18421 actaccagct cggcgtgctg cgcctgcggc tgccgcccct gagcgcgcgc acggaagacg
    18481 ttcccgcact ggcctcctac ctgctcgcca gcgcctcgcc gggacgcgac atgctggttg
    18541 atccggcggc catgtccctg ctcaaaggcc acgactggcc cggcaatgtg cgcgagctgg
    18601 ccggagccat gcgccatgcc tcggccgtct gcgccggcac ggtcatcctg ccccatcatt
    18661 tgccggagag tgtcgccagc gcgcgcgctg cgggcggagc cgtgctagag gaaaatttgc
    18721 gccaggccct caccgcctgg ctggatcaaa ggctgactgc tggcggcgag agcaccccgg
    18781 attacgacac cctgcttgcg gacatcgagc actggatgct gtccgaactg ctggagcggc
    18841 atgaaggccg ccccactcat ctcgccgcag ccctgcggat gaaccgcgcc accctgcgca
    18901 agaagctgcg cgagctgccc ggagcggact gacaccaacc aggctggccg ccgctcattc
    18961 caccatttcc aaaaacaact cctccaggct ctgcccctcc ttctcacgag tcgcgcgcag
    19021 ctccgccatc gtgccgtcga aaacgagcct gccccgcttc aggatgccaa tgcggtcggc
    19081 gagctcctcg gcgatgttga gcaggtgcgt gctcatcagc accgtggctc cagagcggct
    19141 gagatttttc agctcctcct tcaccacacg cgcgtggatg gggtccagcc ccaccatcgg
    19201 ctcgtctatg acaaagacct tcggctcgtg cagcagcgcg ctggcgatgg cgagccgctg
    19261 gcgcgtgccg tggctgagat tctcgatgcg ctggccgctg tgctcatgca gggcgaagcg
    19321 ggtgaagagc gcctccgtgc gctccgccgc gcgggcgggg tccatctcga aaagttcggc
    19381 gatgaagtgc atgaactccg gcgcgctgag cttttcataa aacgcggcca cgtccggcac
    19441 atagccgagc agcgccttgg ccttcatcgg ctccgactga atgtcgaagc cgcacagccg
    19501 cacgctgccg gagaggggtt tcatcagccc ggtgagcagg cgtatggccg tggtcttgcc
    19561 cgcgccgttg gggccgagaa aggcgaacaa ctcgccgggc cgcacttcga gcgacaactc
    19621 atccagcgcc ctcaagtcgc cgtactgcat ggtcaggttt tcgatctgga tcatgaaacg
    19681 cgccggagtt gtaaacgtgg atcggcggct gaaaaggacg gattcttgcc gggcggatgg
    19741 aaagtttggc actggcgcgg gggattcggg gaaaaggtcc gggcatccat cttcgttccc
    19801 tttcaaagac aaagccccgc gacaccatga gaaccccata ctttccctgc gccggagcgc
    19861 gcgcgctgca cgcgcccacg cggcgcgagt tcgtctatgg actcggggca tcgcttggca
    19921 gcgtggcctt cagctccatg atggcggcgg aaaagtctcc agggcctctg gcgcccaaac
    19981 cgcagcaggc gcacgcaaag gcaaaaaact gcatctttct catgatggag ggcggtccct
    20041 cgcacattga ctgcttcgat cccaaaccag cgctggaaaa actgcacctc aaagaattca
    20101 tccgcgaggg caggcagaag tcagccatgg agagcggcaa gcgctactat gtgcgcagcc
    20161 ccttcaagtt ctcgcagcat gggcaaagcg gcgcgtggat gagcgagccg tggcgtcacc
    20221 tcgccggcgt ggcggacgag ctttgtttct tccgcggctg ccaggtggac agcgtcaacc
    20281 acccgacggc gatgtatcaa atgaactgcg gcaaccgctt tgggggcgat cccgccattg
    20341 gcgcatgggt cacttacggt ctcggcagcg tgaaccagga cctgcccggt tttgtcgtgc
    20401 tgccggagat cagctacccg cagggcggca gcgccaattg gggcaacggc tacctgcccg
    20461 cgcattttca aggcacaccc ctgcgcgcca aaggcagccc catcctcgac ctgcagccgc
    20521 cgccgggcat cacacaggag caccagcgcg ccaatctcga cctgctggca aaaatcaacg
    20581 acaggcacgc gcgcgagcat ccctggcatg acgaattgaa ggcgcgcatg aacaactacg
    20641 agctggcctt tcgaatgcag acccaggtgc ccgaggtgct tgacctggcc ggcgaaactg
    20701 agaaaacccg ggagatgtac ggcatcggcc gggacgccac agacgccttt ggccgcaaat
    20761 gcctgctggc gcgcaagctg gtggagaatg gcgtgcgctt cgtccagctc tacgccggca
    20821 cctgggacag ccacgactac atcgagcgcg cccacggcaa tctcatcggc caggtggacc
    20881 agccaatcgc cgccctcatc aaggatctca aacaacgcgg cctgctggat gacacgctca
    20941 tcgtctggtg cggcgaattt ggccgcacgc ccgacaacgg cgtgcgcggc ggcaccgcct
    21001 acgggcgcga ccacaacccc tccgccatga ccatctggct cgcgggcggc ggggtcaagg
    21061 ccggccacac catcggactc accgacgaca ccggcatgga ggccgtggag tgcgtgcatc
    21121 atgtgcgcga cctgcacgtc acgctcctgc gcctgctggg cctggatgac aacaagctga
    21181 ccttttacca cgcgggccgg ttcaagcagc tcagccagtt cggcggtcag gtcatccagg
    21241 aactgatcgc ctgagccgga ccgcggtcac ggcttggccg gcgcgttttt aaacgcgtaa
    21301 atgacgccgt catccgcgcc tgcgatgaca aaatcccccg ccactgcggg cgaggtcttc
    21361 accgccgcgc cgatctcata ctgccacagc tctttgccct ccttgaggtc gagcgcgtag
    21421 acatagccgt cgtcaccgcc aaagatcacc gccttgcccg cgcacaccac cgcgctgctg
    21481 tccacccgcc cgcggcagcg gtactcccac aatcccttcc ctgtgatgcg gtcaatggca
    21541 tgaaaccggc ggtcgcgtcc acccagatag actgcttttt cccaaatggc aggcgcggcg
    21601 taaaactcaa actcgcgctc gccatactcc cacaccgcca tgccatcctc caggctgtaa
    21661 gcgcccacgc ggttgccata gtgcgagaca taaacgacac cgtctgcaat cgcggcattg
    21721 ttgccgatgt aggcaccgac ctcgatctgc cgcttctctt ccccggttaa cacgtcatgc
    21781 acatgcagca cgctgtcaca cccgccgaag acgacgaccc cttcacctat ggccgcgccg
    21841 ccattgatgt agtaccccgt ctcggcggcc cactcctttt tgccggtctt cgcgtcgagg
    21901 caatacacgc tgtaatcata gctgccgatg aggatttttt gctcttttgt ctccgggtgc
    21961 gtgaaggtgt tggaggcagc gtggatttcg gcctgcgtct cgaacttcca cacctccttg
    22021 cccgtctccg cattccaagc ataaacaaag ccgtcctggc agccggccac cacgatttca
    22081 ccggcaaagg aagccgcccc gtcaaaagcg ccccctccct ccgcctccca gattttgttt
    22141 cccgtcttga ggtcgagaca gaaaaaacgg ccctccttgc aacccgcata cacacgcccg
    22201 ccgcgcgcca ccgcgccgga aacaagcatc tctccgcgcg ctttttccac cggcacggcg
    22261 cggtactgcc aggccagttc cattggaaag cgcaactcca cgggcgagac gccgcgcatg
    22321 gcctcgtcac cacgggcatg aatccagtcc ccggcctcct gggcgtggca tgagaaggct
    22381 gcgaggagaa aggccggggc aatgcgcagg gcggtcatgg atggcgggcc gggttgccag
    22441 gtcagtcgag aggacggatc caaatgttgc gcatgcgcac aggcggcacg tttttgtgat
    22501 cctggaggcg tatggacgct ttggccggaa agacaccttc gtagctggtg atgtttttgt
    22561 gcttcgtcgg accctggatg acggtgttgt tctgcaccag gatgccgttg atgaatgtgg
    22621 tgatgcgcgc cggctccagc actttgccag cctcgtcgcg cttcggcgcg gtgaagacga
    22681 tgtcgtaact ctgccactgg ccggcctttc gcacggcatt taccaggggc ggtgtctggc
    22741 cgtaaaccgc gcccgccatg ccgtcggcgt acgtgggatt gttgtcgcaa tcgaggatct
    22801 ggctttcata caggtccatg aaaaagacgc cgccgttgcc cttcttctgg gagttgcccg
    22861 cagtgtgcgg ctcgctcttc cattccaggt gaaactggca gctcgcgaac tcctgattcg
    22921 tccaggcgtc gccgccgtcc acgagcagct cgccattctc gatcttccac tcgcacggcc
    22981 cccactcggc ggtgggcttg ccgtcggggc cttttttctt cgctttaaaa gcctcggtgc
    23041 tcgtgccgtc gaaaagcacg atggcgtcgg agggcggccc gcctggtttc tccgaagggg
    23101 tgacggcggc gggatgcgga cggtctatgt cgtgtacctt ccactggctg ccgggaatca
    23161 caggcgtgtc cgagtagccg gttggtgtgg gcttttcctg ggcgaaggct gcgaaggatg
    23221 cgagcgcgag ggaggtgagg gtgagtttct tcatgggcgg gctgcaaacg gacacagccc
    23281 gcgccttgtt gcaagagcgc aggctgtgtc acgggtgaaa agtccggctc aagcgccgac
    23341 gatgtcgcgc gcgagcttga ccgcgtcctt ttccagcccg gaacgcatgg cgctgtgatg
    23401 ctcgcggatg cgctggcggg tgagcttgcc gaagtagcgg accttgcgca gcaggcggtc
    23461 cacatcgtcg ctggtgagag cagagtcggt gatcaggctc tccgcgcggg cgagcgtggc
    23521 ggaccagacg aaaagctcgg cgccaatgtc caccagcctg cccaaaagca cctggcggcg
    23581 ctccagcgcg ggtccgttga ggaccatggc atgaaagaga gcgcgagcca ggcgccggct
    23641 ctggcggtgg atttcggcaa ggtcgccgcg cagggcggga tggaggctgt cggcggcgtc
    23701 gccggggcct tggggcagcc agagactagg ataccagcgc gcgtaaaaga ggccggcctt
    23761 catggccgcc cgggcgcgtg ttttgagggg cagcgtcgtg ttcacagcgg aggcgccgat
    23821 cttcagatgc gggtccagcg cctcgcgggc gatgaagagg cgcatgatct cgctgctgcc
    23881 ctcgaagatg gtgttgatgc ggcagtcgcg gaaaaggcgc tcgaccgggt cggggcgctc
    23941 gccgcgcgcg cgcagagagt cggcggtctc gtaaccgcgc ccgccgcgga tttgcatcgt
    24001 gtcatccacg atctgccagg cgtgctcgct gccccagagt ttggccaggg cggcctccag
    24061 gcgcacatcg gcgtgcttgt cgcggtccac tagcgcggaa acgtaatgca ccacggactc
    24121 catggcgaag gtgtcggcgg tcatgcgagc gagcttttcg gaaatggcgg cgtgccggcc
    24181 cacgggctgg ccccactgga cgcgctcggc ggcccaggtg ctggagattt ccaggcagcg
    24241 gcgcgcgagg ccggcgcagg cggcgggcag ggtgatgcgg ccggtgttca gcgtggtgag
    24301 ggcgaccttc aagccgcgcc cctctccgcc gaggatgttc tcacgcggca cgcgcacgtc
    24361 cttaaaacgc acgacgccgt tgtaaagcgc gcgcaggccc atgaatcggc agcggtgcag
    24421 gatttcgaca ccgggccagg aggtctcgac aatgaaggcc gaggtggcgt gcggcttctc
    24481 cggcgtgggc gtgcgcgcca tgacgacgat gaggccggct ttgaggctgt tcgtacacca
    24541 gagcttctcg ccgttgatga tgaaatcgtc accgtcaggc acggcggtgg tggtcatgcg
    24601 cgcggggtcg ctgcccacgt ccttttccgt gagggcgaag gcgctgatct cgcctttggc
    24661 gcagcggggc aggtatttgg ccttctgctc ctcagtgccg aacaaaatga gcggctgcgg
    24721 cgcgccgatg gattggtgcg cggaaagaag cgcggccagg ttgccgcatt cgccgccaag
    24781 cagcatggcg gcgcgggagt agtttgtttg tgacaggccg agtcctccgt gcttgacggg
    24841 aatcttgatg ccaaaggcgc cgagctttgc cagctccttg atgagtttgt ccggtatctc
    24901 cccggtccgg tcaatcgcat ccgggtccgc gtgcccggcg agcacggcgc ggaggcggcc
    24961 gaggaaggcg tcgccctggt ctttgtcctc atcggactgg cgggggaatg gcatgacttt
    25021 ggagaagtcc ggcccaccga agaaaatgcc cgcggcgagc cctgtgttgt ggcgttcatc
    25081 gcgcgcggct tcggccattt ccagggcggc tttctgcccg gcggacatct tggaagtgtc
    25141 aatcagcggg gccttggctt caagagcggt ggctgttttc atgatcgtgg aggtgtgcgg
    25201 ggttgctccg cctcgggcac tcgcaccagc cgggtgtctt cagcggcttg tcgttaaaag
    25261 ttgagacaac ctggcagccc ccagggttca aaaacaccgc cctgcccgtg acgctccgcg
    25321 tcttgaaatc aaatgatccg ggcgatttcc gccacctcat ccaaggtcag tcccgactcg
    25381 tcccggcgga ccttccattc gagaagatcg agtatgctct ggcgcgcggc gggccggcct
    25441 tccgggggga aggtctcgac atcgttgtct atccagacaa ggtcctcatc gggatcaccg
    25501 ttccacatga cgttgcctgg atgcgcgtcg aggttgtgca ggccggagtc gagcatgagc
    25561 ctcatgcctg cggcgtattt gcggatgaaa accctgcgga tgtcgggcgc ggtgaggtcg
    25621 ggccgcagga agattttacc aggctggagc ccctccatga attcggcgac gtaaatggaa
    25681 tccactttgg aaaaagggtt ccagttgctg aagctgccgc gcggttcggg aacgcgcaga
    25741 ccgatctccg caagggcgag cgcggatttg tgctcacggt cggcatggcg ctcatcgagg
    25801 aagcgcaggt aatcacgcgc cttccgccgg tagatgattt tgacgatgct gttctgtgga
    25861 tcggtggcga acttgtaaac cgtggccgtc ttgcgacgtg tgaggcgggt gacgtgcgcg
    25921 gggagacggt ttttatctgg agcgagtccg agcaaaggcg ccgccacctg ggcattgatg
    25981 acgcagccga taaaaaacgg caggctgagg gcggttcgga tgcgtcggat catgattcgg
    26041 gagtgtcatg aaacctgccg acgcctgtca gacgccgcag gttgacactg aatctcagtc
    26101 ttgaaacaag gatgtcaacc ggggatgtcg ccagtggaaa gtatgagagg cttgatgacc
    26161 tggacatcct gcggcacgag ggcggcttga agccgttgat acatcgcctc gtcctcgtag
    26221 gtgccgacga tggctccacc gctgccggtg aacttggcgc tggcaccgca gtcgcgggcg
    26281 gtctcgacca ttttgatatt gccctcgctc aactggtaaa gacggcggcg caggtcgaaa
    26341 ttctcgttga gaagcgggcc gatttcatgg ccgcggcctt cgagcagcat gtcgcgcacg
    26401 cgctgcgcga ggcccgccca gtgatacatg gcgctgacca cgtcccgctc cccccggttc
    26461 caacgtcctc gaatgtcgtt gtgaaagact tcggttccct cgctcaagcg ggtggtgtag
    26521 gcgacataga gtttgggcag cagagcggga tcaagctcct cgtaatggcc gtggccgagc
    26581 ttttccatgg aagcgcggtt gaagtccatg aagaccaccc cttcgtaaga ctggatgaca
    26641 cggtcttgca gaccggcggg gatgccaagc tcgtcatttt cgacgctgag aacaagacct
    26701 ggcaaaacgt gcctgggtat ctccacagcg taaaagcgca tcagcgcgcg ccagcaggcg
    26761 gtgatgatgg cgctggagcc ggccatgccg acctggggcg gcacattgct ggaatagcgc
    26821 agggtgaagt tccgcccgtg caggcggatg cgatttttgg cgcagtggtc gaagaagcgc
    26881 ttcacgctgg ctttgagcag gcggatgccg ccgtagtagc cgaactggcg cacatcccgc
    26941 gccagagcct cgatgctgcc aaagacggag tggtcgcgct cgttcggctc gatgcgcagt
    27001 tcctcgctct cctccaggcg gacttcggcg cggaagttgc ggatgatgaa ggagatggtc
    27061 ttgccgaagt aaccgtcgga aggattgccg accaagcccg cgcgggcatg ggcctgggtg
    27121 atgatgggag tggaggttcg ggagtccaag ccgccgatct taggcggtgt tggcctgctg
    27181 gcaacggcgc agcatgggga atgctctggc ggaactcagg caagaatggc gcttaccagt
    27241 tcgccatgca cgtccgtgag ccggaagtcg cggccctggt aacggaaagt cagccgttcg
    27301 tggtcgagac ccatgagatg gagaagggtg gcgttgagat cgtggacatg cacgccgcct
    27361 tcgacgacgc cgaagccgag ttcgtcagtc ctgccatgag tgatgccggc tttgacaccg
    27421 ccgcccgcga gccagaaggt gaaggcgtcc ttgtgatgat cgcggccggg cgaggtcttc
    27481 gtgccctggc catcaatgcc ctggcgcagc ggagtgcggc caaattcgct gccccagacg
    27541 acgagggtgt cctccaaaag gccgcgctgc ttgagatcgc ggatgagggc ggccataggc
    27601 tggtccacat cacgggtttt ggcgcgcaga cgcttgtcga ggttgccgtg gtgatcccag
    27661 tcggagtcgt agagctgcac catgcgcaca ccgcgttcga cgaggcggcg cgcgagcagg
    27721 cagttgctgg caaaggatgc cgcgcccggt tttgcgccgt acatctccag cgtggcgtcg
    27781 gattcctggg agatgtccat gagttccggc acactggcct gcatgcggta agccatctca
    27841 tactggctga tgcgggtggc tatctcgggg tcgccgacga tgccgagccg ctcctcattc
    27901 agcgcgcgaa cggtgtcgag cacctgacgg cggtcctggc ggctgtgacc ttcggggttg
    27961 tttaaaaaaa gcacaggctc cccgctgccc cgaaactgca cgccctgata acggctgggc
    28021 agaaaacccg tgccccaaag agaagtccca gcgccgccag gcggaccgga aagcagcacg
    28081 acataagcag gcaggtcctg gttgtcagcg cccaggccgt aggtggtcca ggcgccaagg
    28141 ctcggacgcc cgccctggcc aaatccggtg tgcaggaaca tctgcgcggg agcatggttg
    28201 atctcattgc tgtgcatgcc cttaagaagg caaatgtcat cggccacagt ggcaaggtgg
    28261 ggcagcagtt cagatagctc ggcgccgctc tggccgtgtt ttttgaactg atatgtggag
    28321 cccgccaggg tcatctcccc gccgatgaaa gcgaagcggc gtcccttgag cagatcctcc
    28381 gggcatttct cgccgtcatg cttctgcagc acgggcttgt ggtcaaaaag atcaagatgc
    28441 gagggcgcgc cgatcatgtg caggtagatg acgcgcttgg ccctcggcgc aaaatgcggg
    28501 ccggtcacag gagtggaggc gggcaggtcg cgcgccagca gcgatccaag cgctgccgcg
    28561 cccatccccg cgccggcatg ggtgagaaag tgccgccgcg tgacggtgag aaagtgcgga
    28621 ctgctgtgca tgagcgcggt aacgcggcgg tgcggggtaa cttgcatcca cggcactccg
    28681 cagcgcagcc aggcgccgcc cagacttgcc aaaagcccgg cctggcggtt aaaaacagct
    28741 catggtaaaa aactccttta ttccttcagc cgccttgatc ctcgccctgg cagccgcccc
    28801 cccgtcctcg gcgcaggagg caggaaaggg aggggccgcg cccgcctccg ccttgcggct
    28861 ttcccgcaag gcgccggtcg tctatgagcg gcagctcaag gccttgctca cccctgcgga
    28921 gaaggtgctg gcaaggcgtg gagacttcga aacaaatggc gccgacggcg tcatccttct
    28981 ggatgagacg ctgattcact cggagccgga cggacgccgg gtgctggttt atcaccggat
    29041 gtttcaggca ctcaatgagt cgggcgcgcg catgactgcc gaggacactt tcagcttccg
    29101 caccctggac cagcgcattc atttggcgga ggcgcagtct ctgccgccgg aggggtccgc
    29161 tgtggaggtc gaggacggct cggtcatcat ggagtctccg cagcacgacg cggcggacag
    29221 cgtctatggg gaccgcggac agatgcggat cattttcccc gcggtcaagc ccggcacggt
    29281 gacgcgaatg gtcgtggtga tcgaggagtt cgatgaacgc attccagggc acttcagtac
    29341 ctgggaaagc tggggcgctt tctggcccac gcgccacgcg cgcacagtgc tgcatctacc
    29401 cgaggacatg gaggcgcgcc tgcgggagac gcgcctcggc gcaggggtgc ctgagcctgt
    29461 gaaaagcagc gcggctccgg gctggaggac ctggacctat gacaagcatc gcatcaagcc
    29521 ggaccgcgcg gagcatggcc gcccgcccaa aagccagagc ggcccggcgg tgtttctcac
    29581 caccctgccg gactgggaga ccttcgggga ctggtaccgc gccctgctgc gcgagcgcgc
    29641 ggaggtcacg gaggaaatgg ccgcactggc gcgcgaatgg acgcgcgcgg ctggcacgga
    29701 cgaggagaaa gcggcggcca tcctaggcca tgtggcgcgc gacatacgct atacgggact
    29761 tgaattcggt ctgggcgccc tgcaaccgcg cgcgccggtg gaggtctgga agtccgcctt
    29821 cggagactgc aaggacaagt ccaacctggc cgcgctgctg ctgcgctccc tcggcatcga
    29881 ggcgcacctg gcactcatcc aaacggagca cctgggacgc gtggagcgcc gcagtcccga
    29941 ctcgcggcat ttcaaccacg ccatcgtggc gttgaggacc gcgcagggct ggcagttcac
    30001 cgaccccacc ctccgccatg tgacacctgg aatgctggcc ccgtccagca gcggcaggga
    30061 cacgctcatc gtcaaacctg actgcatcga gtgggcgcgc acacctgtct ttgaaggagg
    30121 cctggtgcat tatcatgtgg acgcggagcg cgcggaggac ggctccaccg aaggctggct
    30181 ggagtgccgg gccggtggtt tctacatggc caccgaccgc gcctatcatg aaaacatgag
    30241 ccgtgaggag tggaagcgtg acgcgcagca gacgctggcc ggccttctgc ccggagcgca
    30301 gatcattgac ctggacaccg accgttccgc gccggacgtg ctctggcggg cttacttttc
    30361 gcagccaggc cgtgccagcg gcggcgacga gcgcgtgccc gcatccttcc ccgcaggaga
    30421 gccggtgatg ctctccatgg gccaggacga gacgcgcgag acgacgcgct ttctctggcc
    30481 catcacctgg cgggtgacca gcgcggtccg gctgcccgca ggctggaccg cagcggagac
    30541 accgccgcct tttgatctgc acacggaggc ctacgaggtg gaggcgcgct gggagccggg
    30601 ttcgggtctg ctccgcacct tttatgaagg gcgcgtgccg cgcggcgatc tgactccggc
    30661 ggcgcaccaa gtcgtctggc gcggcgcgca atccctggac gcctggctgc aacgaccgct
    30721 ctggctggcc cgctccggcg agaccccgcc cgctccatcc ccggaagcgg ccatcacgct
    30781 ggggaaattc ccactcatgc cgaccggtga gggccagctc gcactcgtgg accggcgcta
    30841 cccggaaaac gggaacctgg acctgcgccg tgccgccttg cgcaaggtgt tggagttctt
    30901 ccccgacgac cctgtgaccg ttttcaacac acgcacgcgc ctggcaaccg cggactggga
    30961 gcaggacaag catgccgagg cggaaaaagc cctgcgcgag atcctggccg cgccctcccc
    31021 gaagatcacc cccgaaatcc tggcctgggg acgctacctc cgcgcgctcg tgctcatgga
    31081 cctcaaacag cacgaggagg ctgcccgaac cttcgagggc ttggccggtg acaccacgct
    31141 cagcgcctac cggcgcgcct gggccggatt ccagctttcg cgcgcacgcg ctgaactgga
    31201 tcaaaaagag ggcgcgctgg ctgcggcgcg cgcgggactc ttgcttgaac aggcagagtc
    31261 cacaccgccg ctgctggccc gcgcggccat gcatctcctg gaactcgacc gccagcaaga
    31321 actgcccccg ctcctggcca gcctcattga gcgcggagag gatctctctg tccccgccct
    31381 caccgccctg gcgcaactcg tcgccggact ccatgaggat ggcaagtccg cgcaggcggc
    31441 gaccctgctc gacatcctgg agaagctgga ctttataaat gagggtttcg cccgcgccct
    31501 ggctgaggcg ggcaacgccc tgcgcggcgg agccgtggcc gcggctctac aaaaagatct
    31561 ccaggcatgg ctcgaagccc ggcccgacac ccccgcgctc aagccgccgc cggatggctg
    31621 gccgcagtcc cgcgaggaat gcgccaaggc attcgaggcc gccgacaacg ctgccagcag
    31681 cgatctcggc caccgcctgg ccttgcgcct gctcaccgcg ttcgagccgg gcacggactt
    31741 cccccgcctg ctctggcgcg cggcggcgca tctggagcac cacgagcgtc agagccagcc
    31801 gtcccagccc tctgatctga tgcgcttttt gctcgatctc ggcctgcgcc tcccgcgcgc
    31861 ggacgatcat tactgggaga tgcgctacct ccagggtgtc gtgctggaaa acctcgtcca
    31921 ggacaaggcc gccgctgcga agcacttctc cactctggcg gatgaaaatg acatgccgcc
    31981 agcctaccag ccctccttta tcgtgcgcgc ggcggcttgt cacgagcact tgcgtgaatg
    32041 ggaaaaagcc gccgggacct ggtcacgcct ggagcgcctg acccagttcg gctccagcgg
    32101 ggacggcctg ctgcgcgccg cccatctgcg gctggagcag ggcaacgtgg acgaagcgct
    32161 gcgcctgctg tccctcgccg gcggaagccg cgacttttat ctccgccaca ccggcatgaa
    32221 ggagaccctt gaagccatgc tgcaattcgc cgacgaaccc gccctggtgc gcgccacctg
    32281 gagtctggag ccggcttggt ggccggactg gaaaaagctg cgcgacactc tcggcatcct
    32341 cgagtcagac attgagcccg ccatccacga cgtggctgct tttggcgccg agatcagcca
    32401 ggccgtgcag gaaaaggacg ccgcccgcgc cggggaactc ttccgccagc tcgcccacaa
    32461 cgcccgctgg gtgcccgcgc atgtcatcaa caccgtctgg gcctccctct accgcatgga
    32521 ggacctgcat ccgcgccatg tcagggaact caaactgttc aatctctccc tgctcgaatc
    32581 cgggcgcgtc ttcaccgagg ataaccgccg cgcgcgctcc ctctactccg tcatgtgctt
    32641 tctggacacc gaccagcaag aagccgccct gaaccacatc ggcgagtact tcaacgccca
    32701 cccgcgcgac gaccatgcca tctccttcac catgtcacgt ctctgggcac tggccgccgt
    32761 cgaagaaaaa aacgagcgcc ccgcctgcat cacacgcctt cgccaggatc tggcagcgcc
    32821 cgctttgcgc caggaccgca tggcgtccgt ccggcagctc gtccgcctcc tgcgcctgga
    32881 ggacatgcaa tccgaaatac gccctctgct ggagggcgag ttaagccacc ccgcgcacag
    32941 ccaggaccag gaggcgctgg ccgcgcttaa agacatgctg cgctccgaaa ccgagggcgc
    33001 cgccgtcacg cggaccgtcc agcgctggct ggagcgccac gcgccaccct ggttcgacgc
    33061 cgtgcagccg ctcagcctgg ccgatgccgg cctcgatgac gaggatgccg atctcgacga
    33121 ggaggcggac aacctgccac ccgtcccgcg ctgcaagctc tggctcctcg ccgctcagga
    33181 gacttccctt gatgctgaag accgcgtccg ctggttcgcc aatgcgctct ccctctggcc
    33241 gcggctcaat gccctcacgc gggaccagtt tcacgccgga gtcagcactc tgctcgacga
    33301 cccgcaggcg cccgccgatc tcaaagaccg cgcccttcgt gtcaccgcca tcgtctgcgc
    33361 cgaccacgat gaaaaagaga cctggcttcg cttctccaaa aaacacgacg acctcatcct
    33421 cgacgagtac acgcgtgacg ttctcaatgt cgtggacctc gtttttgaaa ccggccccgc
    33481 ctctgccgcc agccctgccg cctccacgcg agccgtccag accatcctca aagaaaaaga
    33541 ctcctacctg ctcgaactcc tcgtcgaatg gctcgccgac cgggcactcc ggcatggcag
    33601 gcccgatgcc gcgcaggccg tcgccgacgc gctcgccccc gcgcgcaaac cggccggttt
    33661 cgacgcctcc gagttgcaga ccctgcgcct cggccttgtc aaaaaactct ccgccgtcaa
    33721 acgcctccag cccgtgcatg aggcgctggc ccgcatcgtc ctcgaagaac ctccccgcga
    33781 gcaattgaag ggcgttccag acgattgtga tatcttcggc ggcgcggact acgacgctct
    33841 cggctcagcc cgctggctgg ccgttttttt gcatgagctg cgccacgacc gccatgagcg
    33901 ccaggacctc accgactggc acaccctggc gtcagtccag tcagaactcg acgccgccgg
    33961 caccgcctcc ctgcgccgcc gtctcatcag tgccatgacc accgcgcccg tggaggacga
    34021 caccgcgcgc gccgatcttg cgagccttgc agaggacctc accgacctcg acagcgagac
    34081 cgagcgcgcc cacctccagg agtcgctcca accctggcgc gaggcgcgtg acaccgccgc
    34141 ccattccgcc atccgtctca tcgaggcgcg catcgccctg cgcaccggcg gcccgctgga
    34201 tgtggacgcc atgcttcgcc agatcaaaga ccccgacagc cagaccgaac tgcgctggat
    34261 gcaactgcgc cgcgcccgcg ccaccgaaga tgctgcgctc gcccgcaagg cgctggacgc
    34321 gctcggagcc gacgcgctgc tcgatccagg ccgcatgcac atcgtcatcc cagccttgaa
    34381 acacaccaac aggcaggacg agctggcact ggcggtggag gcaacggaag cggccttcca
    34441 ggaagccctt ggcagggcat ggacgggccg ccacagtggc gcggcgcgtt acgcgctgga
    34501 actggccgga ctgctcggcc agcacgaaaa cctgccgccc ggctggttcg acttttgtct
    34561 gcgcgcctac cccgagcgca tggaccaact caatctggaa tgccatctgg cacaaatgcg
    34621 cggggactgg accgccgtgc tcgccgccgc tgaggaactc gtggggaagt accccacttt
    34681 ctattcccat tactggcccc aagcccgggc gctttggaaa ctgggacgcg ccgcggaggc
    34741 ccgcgagccg ctggccatct tcctgcgtca ttgccacgac gaacccgaac acgccgaagc
    34801 cgggcgcatg ctctccgaac tcgactccaa gcggtaagca cggggcgcgg aatgccccgc
    34861 tttttcctgc cgaagaatga ccgtgcgacc ggcatccggc gcgtggcggg gccgctcctc
    34921 caaaacacca gcactactcc tccctgccct gcgggcgcgc ggctgcggcg ggggtgttgg
    34981 tcaaggtgtc gccgagttca tggcggatgg gttcagcgag cttttggagg cgctccacga
    35041 catcgggatg cgcggcgctg acatcggtcg tttcgccgat gtcggcgcgc agattgtaga
    35101 gagccagggg ctggctggcg actttatatc cgtgccgtgt ggcaatgccc tcaatgccgg
    35161 actgggtgat ggaggcgggt gtcatgttct cccagttcga gggtttgccg tcttttccag
    35221 gggtctcatg cggggtgatg taggggtgct cgaagtgcag cttccagtca ccggagcgta
    35281 tggcctggaa ttccttgccg gcgtagaagg cgaaggcttc gtgcgggctt tccgcgccgt
    35341 ctttgccgag caggacatcg ctgatgtcgc gcccgtcaat tttcagcgcg ggcggctccg
    35401 cgccgagcca ggcgctgaag gttggcatga ggtccatggc ggtgacgagc gtgtcgagtt
    35461 cacgcccgcc gggcacatga cctggccagc gcatgatgca tggcacccgc acgccgccct
    35521 cgaaggtggt gagctttccc tcgcgcagcg gggcggcgct gccggcgtgg ttgccatagc
    35581 tgagaaaggg gccgttgtcg gagaaaaaga tgacgagggt cttgtcgtcc actcctgcct
    35641 ggtggacggc ctccaggatc atgccgacag accagtcgag ctcctcgacg acatcggcgt
    35701 agaggccgtg cggcgagccg cctttgaagc gcgcggaggc gaagatgggc acatgcggca
    35761 tgatgtgcgg cacatagagg aaaaagggct tgtccttgtg ccggcggatg aagctgacgg
    35821 acttctcggt gatgcggcgg gtgaactggc tctggtcggg gtcggtctca atgacacgct
    35881 catcctcgta gagcggcagc ggcggcattt cggcggctag actggggtgg aatttgctgt
    35941 tgtcgttcga gtagggtatg ccccagaact cgtcgaagcc gcggcggcac gggtggaact
    36001 tcattacggt gccgaggtgc catttgccga aggcggcagt ggcgtagccg ccggctttga
    36061 agacctccgg catcatgcgc tcgccgggat ggacaccctc cttgctggtg tggttgagcg
    36121 cgccctgcat gccgatgcgg ttcggatagc agccggtcat gagggcggcg cgcgaggcgg
    36181 tgcagacggc ctgcgccaca tagaaggagg tgaatttcga cccctccgcc gccatgcggt
    36241 cgagatgcgg ggtgcggatg gtctgcgagc cgaaacagcc cgcatccgcg taacccatgt
    36301 catcggcgac gatgaggatg acattgggcc ggtccagcgc ggagagactg acgacggcgg
    36361 caagtgtgag aaagatgtgg agcttcatgc tgcactcgac accacccttc cctgccgcgc
    36421 atctgaaaat cacacgcatg gatgatcccg cgcgtcagga caaggaagcg agaagcttgc
    36481 gcgacacgac atttaaaggg aagcatcaaa cgggccatga cgggcatgtt tgaatcatga
    36541 ttttgtcacg gcttatgggt tgacggcatc ggtgaatttt gcaaggatcg cggcattccg
    36601 cgcaaaatta cgatgcgaga attccaaccc cttttgatgc catgaaaaag attcaaacaa
    36661 accttggcgg agtggcggcg ctgatggcgt ggatgatgac tgccgcgcag gcattttcac
    36721 aatcccagct tggactgacg gaaagtcctc cggccagcgg tttttactcc aacgtggtca
    36781 atgacagcct gaccggcatt ggtgtggcca atgccaaatc ctggctggtg gagctcgagg
    36841 gcggggaccg tgtgacgatc tgggcggtga cgagcgcggt gggttcgaac ccaaggctgc
    36901 gtctctacaa tgcgggcggc acgcttcagg catcgaacaa cggcacccag caaggcgaga
    36961 caatgatcca aagcttcaca gtgacaacgc cgggcagcta cacgatccag ttttatgcgg
    37021 acagcgtggc atcggcattt cagatgcggg ttgacatggg gcggggtttt gcgctggagt
    37081 cggagccgaa cgacactcaa tacacaccca cgctgcccac actgactcct accgggggcg
    37141 gcttcacaac gaaggcggcg ggagtgctga aggagggcgc ggattatttt gatctcggca
    37201 cgctggctgc agggaactcg gtgaatgtca gtctcggcct gccggctctc tcgacactgg
    37261 cggctgggga tgtggagatg acgctgtttg cgaaaggctc ggaaggatcg cccgtggcga
    37321 cgacatttgg cgcggtgctc aacggggcga taccttcatc cgctgagtat gtgctgcgct
    37381 tgcgggtgaa tcccgaactg tccggtcatt cgctgcgctt tgacggaacg aacgatgatg
    37441 tggcgctggg caatccgaac gagctgcgca tcacggggga tcagaccatc gagatgtgga
    37501 tcaagccgga cgacttcggc gggcggcgca atccctggaa caaggcctac ggcggcgagg
    37561 gcacgatgac gatcgagacg gacggcggca tcaattattt ttacgggaca aacggcggag
    37621 acaacaaccc ctaccagggc ttcttttcca acaagcttat gaccgccggg gagtggaccc
    37681 acattgcgct ggtgcggcgg ctggacagcg agccgaggaa gcttttctgg tacttcaatg
    37741 gcaaactggt gaacgaggtc aacgcatcct attttccggc tgtggcgggt gctttgaacg
    37801 cggtcatcgg atcgggctac gcgggcaggt tccgcgggct gattgacgaa gtgcgcgtgt
    37861 ggaatgtggc gcgcagcgcg gcggagatcg ctgcgaacct ggacaacgaa ctgaacggaa
    37921 acgaggcggg gctggcggcc tactacaagt tcaacgaggg atccggcgcc gcgctggcgg
    37981 atgccacggc gaacgcgctg aacggtgtca tcaatggcgg cgcggagtgg gcgggcacgg
    38041 ctggcgcggg cgccttcagc gcggcgagga cgggattgat ggcgcagtat gtggcgagtg
    38101 tgacggtgac tgacgcggcc atcccctcgg tggtgagggc cgcgcctgtg ccggtgtttg
    38161 ggcacacttt tgagggggcg cctgatttga tcatgaaccc cggcagcatg acggcttatc
    38221 gcggccagaa tggaacgagt tttcatgtgc aggtgaccgg cggcggcggg tcagtgtggg
    38281 gcacgggtgt ctattcagat gacagcgcgc tggcatcggc ggtccgccac gcgggtctgc
    38341 ttgccaacgg cgagacaggc ttggtgacgg tgaccatcct gcctggcggg tcgagctaca
    38401 ccggcacgcc gcaaaacggc gtctcctcat caaacaaggg cagccatcct ggaagctaca
    38461 gcctcgcggc ctacagcggg ccggcccccg cgctcaacgc ggtgtttgcg ggaatgcggg
    38521 tggacttctc tgaaagaatg ctgccctcca cgctgcaaaa cgcggccaac gtgatcctca
    38581 cccacgctgg cgctgacggc gtgctcgaca cggcggacga ccatgcccac accttgagca
    38641 actcttatga cgggcttgcc acgacggcct acttcacggt cgtgaacgag ccgctgcagc
    38701 ctggtctgca ccgcctgcgg ctcaccaccg gagtgcagga ccgcgcgggc aacggactgg
    38761 cggcggacag cgacaccctt ttcaccgtca ccgccaaaac gcctttcgtg gtggagaatc
    38821 gtgacaacaa tgaccgcctg aaagccacac cgctgaacgc gccagcggcg gcggtgtttg
    38881 accgctcctt cacttacacg catgacatcg ccgccggcac gggaccgcac cgtctgcggc
    38941 ttctggatct ggacggcgat ggagcgaaag aagccatcgt ggccgctcat aactcaaatc
    39001 agatcagagt ttaccctggc atcgcgaacg gctcatttga tccgactagc acgaactacg
    39061 caaccgggtc gaatccttgg gcactggagt tgctcgacta tgataatgac ggacttctgg
    39121 atgtgatcgt ggtatgccat tcctcggatc aagtccgcct gcaccggaac aatggggacg
    39181 gcactctttc cgcggacggt tccattactg tgggagacgg acccattcac attgtgaagg
    39241 cggacttcga cgaagacaca atcaccgact tggccgtggc caacttcaac accggaacgg
    39301 gcgggcgcag catcagcatc ctgctggcag atggatcagg aagcttcaca gaaagcaagg
    39361 tgtccgtcgc gggaaatgtt tttcgtccgt atggctttag cgcgggggat gtgaatgggg
    39421 acggacacga cgacctggtc gtgggagatt tcgacacgga catgatgcgt gtctttcagg
    39481 gcaatggcga tggaactttt gatgcgcccg tggattttgc catcagcggt gtcaatccca
    39541 cttccattgg actggccgac tttgaccatg acggtaatct ggacgccatg gtgatggcgg
    39601 agacgagcac ccttgtctca attttgaaag gcaatggaga cgggactttt caagcatttg
    39661 ccacatggcc gctggcagtt aacgccagcc aatatttcgc cgaggcggtg gatgttgatg
    39721 aagacggctg ggtggacttg gtcattccac gcacgaacgg tctggaagtg gtgtataact
    39781 cacgctcgac cgttgcacca gcttttaccg gcgcgttgcg ctaccagatc ggatacgcgc
    39841 tgggcttcgt gcttcaggat gtgaatggag actcacttcc tgatttggtt gtcaatgaat
    39901 ggaacagtaa ccgtattcgc actcttctgg gcaaccctca gattccgctg acacaggacg
    39961 cgattctggc aaggctcggc aacacgcatg gcagagggcg catcctcgac ggtgacgtgg
    40021 actggttctc tttcacgatt gacgggccga agcgcgttgt tctggcggct gacattccct
    40081 tcaatccagg ctcggccggg ttgaactata acatctatga cgcggatggc gcgcatatca
    40141 cgggattcaa cgcggcatcc aatggcattg gccagacagc gcagttgaac ctgaacgcgc
    40201 cgggcactta ctatgtgcga gtggcgcaca actggggtta cacgggcgag taccgcttcc
    40261 gtgtctcggc tttcgacgtg ccgaccacga tcgagagcga ggcaaacaat tccatcggcc
    40321 aggcggactc cgtcgcgctg acgcttggag tcggctcgct gacaggggcg gcggcgggtt
    40381 acattgccgg agatgacacg agcggcgact actggtccct cggcaatctg ggcgcgggcg
    40441 ccgagatcac gcttgatctg cgcatcccgc aaagcagccc attgtcgggc aggatggcca
    40501 ttttcaagac cgacgggact caggtggcgg tcagcgacgc gggcgatccc caactgagtt
    40561 acgccgtcac cgctggcaat gagagcgcct attacttccg ggtcttcgat gccgccagca
    40621 cgcgcggcct gcactcggag tacctcgtgg atgtgaccat cacggaccct gagcagcctt
    40681 tcgtcacggc tgtctccctg cctgcggagg gcgccacagt gtcatatgcc ggtccgaatt
    40741 tcaccgtcac cttcagcgag gacatgctgg cctcgacggt gacgaacgcg gcgaactacg
    40801 acctgcgcgc ggcgggagtg gacggcgttt tcggcacggc ggatgatgat gtttacacag
    40861 tggtgcccca agcctacagc gccggactgt ccgccagcta ccacatcccg gacggcccgc
    40921 tccaacccgg aaactaccgc tttatcgctg gaaccgggct gaccgacaaa ttcgccaacc
    40981 cattgctttc cgcccacacg cgcctgttca ccgtcgccgg agtgccgggt tatgtttttg
    41041 aaagccgcag caacgagact ttgcaaacag cggactcgat cagcaccagt cccggcgcgg
    41101 cgcatgacgg atcgtttacg tcggcgggtt tcacggcgac tggcaacggt ccctggggat
    41161 tgaagctgct tgatcttgac ggcgacggac acaaggaagc catcatcgcc ctgcataacg
    41221 cggcgcagat ttcagtgctg cccggcaacc cggatggaac tttcggcgct gcggtgaatt
    41281 acgccagcgg cacgaatccc tgggacatcg agctggtgga tgtgaatagc gacgggcggc
    41341 tggatgtggt ggtctcctgc cacggcagcg accaggtgcg catctacaac aaccccgggg
    41401 atggcacgct gaccgctggt ccggtcatcc ctgtcggtga cggccccatc cacatggtga
    41461 aggggaactt caatggcgac gccttcaccg atctcgcggt ggtgaaccat ctgactggca
    41521 cgggagggcg cagcatcagt attctgctcg gtgacggcgc cggaagtttc acggagagca
    41581 agatcacggt cagcggtcaa acggtgcagt tttacgcgct gacggccggc gacttcaacg
    41641 gcgacggcac ggacgacctc gctgcggggg attatgtgct ggatgacatg gcggtgttcc
    41701 tcaacaccgg cgccaataac ggaacctttg cgcagccggt gttttatccc gtggaagaca
    41761 cggagcccac gggcgccggt gtggcggatt ttgacgggga tggccacctc gacatggccg
    41821 tatgcaccga gttccacaac cgcgtcagtg tgttgaaagg cagaggggat ggaacctttg
    41881 agccgttcac gacctttgat gtggatggca actccaacat gttttatgtg gagtcgcccg
    41941 acctcaatgg cgacggctgg ccggacctgc tcatcgccag gtacaacggg gtcgtggttt
    42001 gccaaaaccg cggagatggc acgatcaact tttcagcacc ggttcgttat tcggactcca
    42061 gcaacaccgg cggtcttgcc tggtcggatg tgaatgggga cgggcgggtg gacttgctgg
    42121 cgaactccta caatcaaaac cgtctttatg tttggaccgg caactccagc cagcccttgg
    42181 cggaggacgc cgtggtggcg ggcatgcgtc acggcgcgct gcgcgggcat ttgacgacgg
    42241 actcggatgt tgactggtac tcgtttagcg cgctggtgca ggaccggctg ctcatcacca
    42301 cggaaacgcc cggcgcgcaa ggctcttcag gcaggaacta caacatctat aatagcgtgg
    42361 gcacccacat cggcggcttc aacgcgaatt ccaacggcta tggggaatgg actgtcacct
    42421 tgccgcagtc cggcacttac tatctgcgag tggctccgaa ctggggaggg acggcggaat
    42481 accgggcgag gctgactttg gctcgtcccc cagtatttgc ggaagtggag agcaacaatt
    42541 cggtgagcca gttcaacacg cctccgctgg cgctgtccgc aggccagctc accacgacgg
    42601 ttctgggggt gaacatgcct cctgacacct cgggcgactt ttttaatctt ggtaatctca
    42661 ctcccggcac gcaggtcaca gtttccgtct tggcgccgca acttgcggtg gacaatttgc
    42721 cctccatgca ggcgcttgaa ctcaagcacc gctggagctt tggagaagcc gtaggagcca
    42781 cggagttcac tgattctgtg accagcctgc ccagcgccaa tctgctgggc acaggtgctg
    42841 tggtgggcgg aggacaggtc gtcttgccag gaggcgcgtc agggtccgca ccctacatag
    42901 acctgcccaa tggcatcgtt tccagccgga caggcagcgc caccttcgaa ggctgggcga
    42961 cgatcaatgg ctctcaaaac tggtcccggc tgtttgattt cggcacaggc acggctggcg
    43021 agcttttgag tccgggcggc tcggccacag gagggcagca ttttttcctg tctgcgcagg
    43081 tcagcggcac attgaccagc aatcgcattt ccatcaactc gggcgcctcg aactccgcgg
    43141 attttggcgt ctcatactcc gccgggcagc agtttcatta cgcggtggtt tatgagtccg
    43201 ccggggcttc ggggggcgtc atcaaatatt accgggatgg cgtgctgcgt ggttcgtttg
    43261 gcacggcggc catgctgaca gggatcgccg atttgaacaa ctggcttggc cgctccaact
    43321 ggacatccga tggcaatctc aacggtgcat acagcgaatt tcgcgtatgg gatggagctt
    43381 tcaatgatgc gcaggcagct gcaagcgccg ccgccggccc ggacactgtg gctggcgcgg
    43441 tgccgccagc gcttgccgcc cagccatttt tgacgctttt caaatcggat ggcacacaga
    43501 tggcgtttgc tgctccagga actggtcctc ttcagttcac tctcggcgcg tcggatgccg
    43561 cctcctacag gctgcgcgtg cataggaatg aacgctcggt cacatccctt tatcaggcgt
    43621 ccatcatcat tgaggacgtg acgccgccgt tcatcacctc gaactccctg cccgctgaag
    43681 gcagcaccgt caccttcccg catcccgtct tcaccctggg cttcagcgag gacatgctcg
    43741 catccaccgt ggtcaatgcc gccaactacg agctgcgcag cgccgggccg aacggcgtct
    43801 tcgatgacgc ggacgacgtg ccatacaccc tgtcaccctc gtcctacacg agcggccttt
    43861 ccgcgactct gcgaatcatg gacggaccgc tgcaagccgg ggcttaccgc ttcacagcgg
    43921 gcaccggatt gaaggacctc ttcgagcggc cgatggatgc cagccatgtg aggttcttca
    43981 acatccagga tgtggccggg tttgtcaccg aagggcgcaa caacaacacg ctggccacgg
    44041 cggaaattct atccggcatc accacccccg gcggattcga cggttcctac gccgtgcagc
    44101 cttccttcac ttccggcggc agcgcgccca tcagcctgct gctggttgat ttgaacggtg
    44161 acggcgaaaa agacctcgtc gtctcgaacc gcgactcgaa caatataacg acccgccttg
    44221 gcgccggcga cggcactttt ggcgcgccgg tgacatacgc caccggcagc cagccgcatt
    44281 acatgtgcct ggtggacttc gacaaggacg gcctcatgga cgtggtggtg ccaatctaca
    44341 atgccgacca gacgacgatc ttccgcaaca acggaaacgg aaccctgacc ctgcacgcca
    44401 ctcttgccgc cggtgacgga ccgctttccg ccgcagccgg ggatttcaat ggcgacggca
    44461 atcccgactt tgccgtgacg aaccatctca ccggagtcaa cgggcgcagc ctcagcattt
    44521 acctcggcga cggactcggt ggtttcaccc acagctttgc cggcgcgggg ctttccccct
    44581 catggcggcc atatcacctc gcggtggcgg actttgacgg caacggtctt tcagacatcg
    44641 cctcagccaa caatgacagc aatgacatcc tggtcttcct ggcgacaggg gccggcgcgt
    44701 ttggtgatcc tgtgcgccat gccacggatc gcaacaaccc caccggcatc gcggtggcgg
    44761 acttcaacgg tgatttcaaa gccgacctgg tggtctgcta cggcagcagt caaaacgcgg
    44821 tcaacatcct caatggcaac ggcgacggca cgttccagcc tcatgtgaac tggactctgg
    44881 acggcaaccg caaccagtac aacatgcgtg tgggcgatct gaatggagac ggctatcccg
    44941 acattcttgc gccgcgctgg ggcgctttgg tggcccgcca gaacaaggcc actcccgttc
    45001 tcagcttcaa ttcagcctcc atctggacgg agttgaacca ggtgctcgat gcggccgtgg
    45061 aggacctcaa cggcgacgga atgcgcgaca tgatcgccct ttcgcacgac caggccgctg
    45121 tcagagtcta tctgggccgc gggtcgttca atttgaacgc cgacaccatc gttgccggca
    45181 tgcgccacgg ctacggacgc ggccacctcg aaacggattc ggattcggat tactaccagt
    45241 tttccgcgaa gggcggcgag cgtctcatca tcgccgctga aagccccgga gcaccttcaa
    45301 gcagcggctt tgatttcaat ctctacgacc accgcggcgc gcatatcctg ggttcctcca
    45361 gcatcgccag caatggctat gtatacaccc agccattcct cattccatat gatggaaggt
    45421 attatctgcg tgtttccccc tggcactaca gcacccatga atatcgtttc cgggtcacca
    45481 cggtggatgc gcaaacgcag atcgaggcgg aggtcaacaa cagcaccggg caggcgacgc
    45541 tcgcggcctt caatgcggcg tctgggtcca ggtccgcgaa aatcttcggt cacatcgccg
    45601 ggaacgacac atccggcgac ttctggagcc tgggaaatct ggcgggcggg acgcagatca
    45661 atgtgacgct caaccgcccg cagaacagca ccttcaccac cggcctgctg cgcatcctga
    45721 acgccggggg ctcggaggtg gcctcctcag gcgcgagcgg gctgaatgtc agccacacgg
    45781 tggcgggcgg cgcggagggg cagtattttc tgcatttttc cgagagcgcg ggcaatcgca
    45841 acctccactg catctaccag gcggcgctct cgctcaccga cgcgctgccg cctcagatca
    45901 cgggaaccag cctgcccgcc gagggggctt cgacactggc tttcatcaac agctttactc
    45961 tctccttcaa cgaggacatg ctggcctcca cggtgacgaa tccggccacc tacgcgctcc
    46021 gcgaggccgg agccgacatg gtgctgggca cgagcgatga tgtgatttac ccggtttcac
    46081 ccgccgccta ttcatcaggg ctgagcgcgt cgttcaatct cgccgcgccg ctgcctcccg
    46141 gcatataccg cctgacagtg gccgggctga aggacaccta tggcaacaac atgcccggcg
    46201 cctttgtcag gaattttagc gtggctcaag tgcccggctt cacgacgatc aatccaggca
    46261 gcaacacgcc cgcgacggcc acggctcttg tgcaggtgga agaacctgac ggtctgatca
    46321 caggagccgg gcgcggacga cgcacttcct ccggcgacac gcactactgg tcattccagg
    46381 gtgcggcggg ccagaagctc accttcgaca cggagttggt cggcgcatcc ccctccactg
    46441 atctttactg gcgcatccgc cgccctgaca actcagttct gtgggagggc aacataggca
    46501 acaacaacca gggcttgttt gccactctaa cgctcaacgc caccggcacg cactatgtcc
    46561 aggtgaacga atggaacacc tggcaggagg agtaccgctt caggctgtcg ctgattgatc
    46621 cggcgctggc tcaggtcgag atcgagtcga acaacaacat cgcctccgcc acgccgctga
    46681 ctctggcgcc gatgggggac atcagcgtgg cgcgcgcggc gggcatcgtt cacacgccct
    46741 cggaccttga ttactacgcc ctgggcgccg tcccggcggg caagacgatc ttcgtttccg
    46801 ccaccctgcc cgccaccagt tccttcctgc ctgtcgtggc ggtttacaat tcctcgggcg
    46861 ttttcatgag cgagatcaat ggcgcgcctg gcgacggctc cgccgaggtg cgcatcaaca
    46921 caccgggtga ctacttcatg ctcgtgcgct ccacgggcgc cactgggaac tacatgagcc
    46981 agtatgtggc cgagttgcag gtgcatgaca cctcctcggt ggcgattcca aacctccagg
    47041 tcaccgaggt ggtgctgccg ccggaaatgg gcctgcaaag cggcgacacc tacacttatg
    47101 agttcaccgt gaccaatgtc ggctcggctg acattcctga cgttccctgg agcgaccggc
    47161 tcgtgctttc gaaaaacctc atttttggcg accaggatga ttctgaagtc ggtgtttttg
    47221 tccgcaacgg cggcctgggg atgggcgcgt catacaccgt gaccggcact tcgacgctgc
    47281 cccagggcgc ggtgggggat tactatttga tcgcccgcac agacttcacg aaccaggtgg
    47341 acgaggtcat cctggagaat gacaacacct ccgccagcgc gacgaccttc cctgtggcgc
    47401 ttgctccata tccaaatttg gtggtgcaga atctcggagt cacaggcccg gtggcggacg
    47461 agttttcgat cacctggaat ctggcaaaca cgggcaatct ggcggccccc gccggattca
    47521 aggagcgtgt gcgcgtggtc aacacaacga ccggggccac tctgctcgac gaactgcgga
    47581 caccgggagg catcagcgcc ggcggcagcc ttgcacgcga cgccagcgtg gtcacgactg
    47641 cggccggggt ctatcagatc agcgtcaccg ctgacgctca aaacgatgtc tatgagcacg
    47701 acggcgtctc gaacacaacg gcggaggtga acaccgcgca gaccagcttc accatcctgc
    47761 aatactggac ggtgagcgtc tccacacccg atcccgcgcg cggcgcggtc agcggcggcg
    47821 gcaccttcct cgcgggcact ccggtcacgg tcaccgccac tcctgacacc tccctgctgc
    47881 cctggcagtt tgtgcgctgg acgcaaaacg gctcctttgt cagcgccagc gccggttatt
    47941 ccttcaatgc caccgcaaac cgcaaccttg tggccgagtt cgcgctgccc tcctatcaag
    48001 tttcggcttc catcactccg gcaggcgcgg gcagcgtggc gggcgcgggc ttcttccagc
    48061 acggctcgaa cgcccagctc accgccaccg ccagccaggg gtatttgttc ggacgctggg
    48121 aggaggcggg agccacgatc agcacgaacg cggtgctcaa cctggctgtc acggggccgc
    48181 gcatcttcca ggcggtcttt gtcgaggcca atccgactca tgatgtcgtg acggcgacga
    48241 atccccccgg actcggagtc accgtgggcg acggtacttt caacaacggc cagtcctcca
    48301 atttctccgc gagcgccagc gtcgaggccg gcgataccga gtacctcttc gaccactgga
    48361 agctcaacgg cgtcttcttt ggcagccagc gcgtcttcag caagaccttc agcacactcg
    48421 acgcgccggt gatgaacttc gtggcgcatt acaccagccg ctctctcaaa ccggtggtga
    48481 cgcaggtgac gagcaactat gccaatccca ttcgcgccgc cggcaatgtg cagtttaccc
    48541 tgacttttga ccggactatg aggaacaacg tcgagccggt cctggtcctc agcagcgcca
    48601 acgcctccga gaccgctgtc atcccgcccg gaggctcgtg gatttccgcc acgcagttcc
    48661 gcagcgcgga cacgaccttc ggcgcgggca atggcggcgc ctatgtgttg aatgccagcg
    48721 cggccacgga ctcgaataac cgcgtcatgg agccggcgga tgtcttctcc tttgacgtgg
    48781 atgtgacacc gcccccgaat ccggtgctgg cattgtcttc cttcactgga acaagcgcca
    48841 cggtgaactg gatgggttac gccgcgcccg cggacttgaa cgcgttccgc atgtacctcc
    48901 agccggccga cttcgcctcc gtggccggta tcccggcggt gagcggtcta ggagccgcgc
    48961 cgcgctctta cacatttacc ggtctgcagc cggacacgca atattacgcg gccatcaccg
    49021 ccgtggatgt ggcgggcaat tccgaccctg ccgtcaccac gctgccggtt ttaatggaaa
    49081 gcacggtgcc accaccggtc aattttctcc tcaccagtcc gacaccggag agcgcgagac
    49141 tcaactggaa cgcctacaac acagccgccc tcatcggctt ccagggctgg cgtgtttata
    49201 tgcaagaaac gcctttcgcc gatgtcacag ggctgactcc tgtggcgcag ctcgccgcga
    49261 atgtgaagac acatgatctt gccgggctgg accgtagcaa gacctaccac atcgctgtcg
    49321 tgggttacaa caggctggat gaattcgatc ccgccgtcac cacccaggcg tggaccgacc
    49381 cgctcgccgg gcagatcacc gccaatctca ccattggcag cgcgggcgcc gatgaggtgg
    49441 tcgaggtttt gcaatccatg atcgtcaggc cgggagccac tctcactgtg cttcccggaa
    49501 cgactttaaa attcgctccc ggcaccggcc tgaccatcga aggcgcgctc gtggccgaag
    49561 gcacgccgct tcgtcccatc aatttcatgt ccagcaccgc cacaccggcc aagggtgact
    49621 ggaatggcgt ggtgctcagc aataccaacg ccagcacacg tctcgcccat ctctggctgc
    49681 gtcatggcga aggcgtgcag gtcgtgggag gcacgcctca gatcagttcg ctattctgcg
    49741 tgcagaacga gggccagggc atcgccgcca gcggcaatgc gaatgtaagc gtggcaaaca
    49801 gctttttcgc tttcaatgat cgcggtgtcc gcgcctcggg caatgccctg ctctcggtca
    49861 cgggttccgt gatcaaaaac aacagcgttc aaggcgcgga gcagtccgcg ccggccgttc
    49921 tcgacatgca ggggaactgg tggggcagca gtgcggctgg cgacatcgcg gccatgatcg
    49981 cgggcacggt ggacacaggc tctccgctcg cgggcgagcc tgtgctggcc gcgggtttcg
    50041 ccacggcggg cggcatcacc agcacgggcg tgcgcaatct cgccgtcgtg ctcgcctcgg
    50101 cgaatgccac cgccttccgc ctgagtgaaa actcgctctt ccccggcgtg ctctggcagg
    50161 aaattttccc tgccggagag acgaacactt tcagcccctt tcccttccca ggcagccatc
    50221 tgctctccac cggcgcgggc ttgaagacgg tgtatgcgca gttccgcagc atcaccggcc
    50281 agaccagcgc ggccatcccg gtgaatgtca cgctcatcac caccgggccg gtcatcaaca
    50341 ctttcagcct cgcggacggc caggtcatca cacggcctct cgccgtcacc ggcagcgcca
    50401 cggccgctct cggaatgaat ctcatccgtc tctttgccaa tgacacgctg ctggccagcg
    50461 cggcgggagg cagtctgaac ttccagtggg acatgcgccc gctcaatgcc ggaccctacc
    50521 ggatgaggct gctggcgcgc gacaacgcgg gcaacgagtc cacccgcgag gtgaatgtca
    50581 cgcttgatcc ccagccgccg cccgctccgg tcatcaccgc cccgttgaac aacgcggttg
    50641 tcgccacgcc ggcaatcacg gtttccggca ccgccgagcc tggtgtgacg gttcgcctga
    50701 cccgcaacag cgcgccgcag ggcagcatga cggccacggt cggcggcgcg ttcagctttg
    50761 ccaatgtgcc gctcgtggaa ggggcgaacg cactcatcgc gaccgccgag gatgtggttg
    50821 gcacgcgcgc ctccacggcg gtgaacgtgg agctggacag cgggccgccc gctcccgtcg
    50881 tgctccagga acccccgatt tacgactcct tccgcggatt gatcctgcaa tggggattct
    50941 ctccatccgg cgagcgaccg gtcaaataca gggtcttctg gcacaacgcg ccttttgcca
    51001 gctccgcggc cgcaagcggc cagagcgcgc tgctgaacgt ccagtcctac actctcacat
    51061 cggcgcctga tggagatttg cacatctccg tggtcggcta cgacgcggct ggaaacgcca
    51121 gccccctttc caaccagatc gcctacactc tcgaccgcac ggcgccggtt ttcacagtca
    51181 gttatgacaa ggccatgccc gccggaccgg ggccgctcac cattgtgctg gagtcgaacg
    51241 agccgctggc cgccacgcct ttgatgacca tcaagccgaa cggcctgaac acgcccatct
    51301 ccgtgcctct caccaagacc agcggcacca cctacacggc cacttacaac gtcacgaatg
    51361 tcagcgcgcg caccggcgct gccgtagtaa gcgtcaccgg caccgacctg accggcaacc
    51421 gcttcagcgg cagccccgga ggcaccgccc tgagcttcga tgtcacgccg cccgcaggcg
    51481 cgctcacttt tgaccgtcca gttccgataa aaacggtcag cgaggccgac cccgccgtgg
    51541 cggaggatgt gagcctcagc ttcaatctca ccctgagcga ggcgcccaaa cccggcacca
    51601 ctcccagcct caccttcact ccgcccattg gcgcggacat cgtgcctgcg ctcaccggct
    51661 ccggcgtgaa ctggaccggc agcatcctgc tcaccgcctc catgggcaaa ggccagggtc
    51721 agttcgtttt cagcgccacc gacgcccgcg acaatgtggg aagctctgtc acgcccaacc
    51781 agcttgagat ttacaacacc gagcatccga acgcgcccgg catcccagtg aacctggccg
    51841 ccaccacact ctccggcggc aggatcagaa tcacctggaa ccccgtcgcg gacgcgcaca
    51901 gttaccgcat ctatcgcgag cttgggaaca cgggtgtgac tcccgtggtg cttgtggcgg
    51961 acggcattga agaaacggaa tggacggatg agaccgtgcc ggaggacggc atttatcgtt
    52021 atgccatacg cgccttccgc gtgagcatcg aaggcaatcc gagcggcacc ctcaacgcct
    52081 tgagcgaccg cacaccgccc aacgcgccgg aggacctgga tgtcacgctc accaactccg
    52141 gtctgcgcat cacctggaat ccgccctccg acggtctcgc gccgcaccgc tatcatgtgt
    52201 accggaacgg ggtgaaaatc cgcactttga acagcgccca gcctgtgctg gacttcccgc
    52261 ccaagggact gctgcaatac gaggtggccg cggcggacag ttacgagaac gagtcgcgcg
    52321 cgggtccggt gcctttcgag tttttcgtgg acgccgtggg cagtctcctg gcccaggtca
    52381 accagggcca gcccaccgtg ctgagctgga ctttcaatga tcctgccggt gtgggttaca
    52441 atgtctatcg caacggggtc aagcaaaacg gccagcccat tcctgtggcg acgaagactt
    52501 tcaccgatct gctgccgctg cctcccgccg agtctgtgga ataccgcgtc acggcagtgg
    52561 acaatctctc tcgtgaaagc gcgccgcgca ccgtgcgcgt gctgcctgtg acctggagcc
    52621 tggtcatgaa cccggatgac gagggagccg cccgcgtctc ccgcactcgc tactttgaca
    52681 attaccggct caccatcctc aacaaggacg ccgccactcc gctcgcgctc aacgcggtgc
    52741 aggtcagccg cacgctgggc ggaggcgcgg tgaccgcttt cacccagccg gtttcattgg
    52801 aaatcgccgc cgcctccaat gaaagcctgg acattcccgt gcctggacca gagttcgacg
    52861 cgcaaagcca gaacctggaa atcagcgtgc gcgccgcgcc caacgagggc ggctcgctgg
    52921 tggcttacac cggatcggcc tcgaacatcc ttgcggctgt cgagccgcct ctgatgctgg
    52981 agatgcgcgt caccgagcct cccgtggccg gcggcctggc cgaggtcaag acccgcatcc
    53041 acaatcgcgg ctacgccacg atggaggtcg tgctgggccg ccagaccggg cagcagccgg
    53101 gcgatctttt catccaggtc ctcgacgagt ccggcgcgga gatcagccgg agaaatttca
    53161 acggctttgg cgcgcccggc ctgttgatcc gcccgaacgg cgacgcctat gtgagcatcg
    53221 ctccgggggc ctcggtggaa gtgtcggtgg agggcgtttt gattcccatc gtgctggccg
    53281 agacggggcg cgccaatttc cgcgccacgt taagccaggt cttcgccggg ctcggcaccg
    53341 cctcccagcg tgtttccggc ccgctgacag gaacgctgca gagttccgtg attgagacac
    53401 cctacttcgg ctcggtaagc acggagaagg acatcttcgc tggagacgag ccgattgtca
    53461 tcagcggcca ggccattgac cgtgccagtt ccaaccctca aggcaatgtc ccgatgcgca
    53521 tcggcttcgc ggcgcgcggc gcggtgtggt atcaggatgt ggaggctgac ggcgacggca
    53581 atttcagtta cagttaccag cccttctccg gtttcgcggg cgagctgacc atctgggcgg
    53641 ctcatcccga tgtgtttgat gtgctggcgc agaagaccgt gcgcatttac cgcatctacg
    53701 ccaccccctc cttcggcaac atccgcatgt cgaagaatga ccacctggat ttcacgatcc
    53761 agttgatcaa tccggcggag ctgcccctga gcggcgtcgg cgtcaatttc agggcctatc
    53821 gtctggatgt tgacggggtc acggagattc cgatacccga ggtgacctgc gacttccagg
    53881 ccgccgggcc gcccgccggt cttgcggcga acaagcgcac accagtgaac ctcaggctgg
    53941 cggccgcggt ggacgcgccg gacaacgcga acattgagat caccttcacc acggcggagg
    54001 gcgcttcgac ccggttcgcc ggaactttgc agttgcttcc ggccgtcccg attctttcca
    54061 tgaccagccc gcgccaggga tatgtggatg tgacggtgaa caaagggcag atcgtcagcc
    54121 agcaggtcac cctgcaaaac ctgggcaccc gcgcgctgct tgatgtcgag ctggttccgc
    54181 cgcagctcat cccctggatg cagatcaacc tgcctgtcaa cgcccagggc aatgccgaac
    54241 ttcccgacct ggatgtcggc gccaccgtca ccttcaccgt ggcttttgtg ccgccggaaa
    54301 acacgccgca gggctacgtt caggacgtgg tcaagatacg aggctcgaat gcgatggccg
    54361 gtttcgactt caatctttac gccctggtca gctccgaggc caaaggcggc gcaacttttc
    54421 atgtcgagaa tttcctcggc caggaggtgc ctaacgccac ggtgcgcatc cgcaacagca
    54481 tcagcggcca ggaagtgggt ccgctcagca ccaacgccag cggcgacgcc acctttgccg
    54541 acttgaccga gggtctttat tcctggcagg tcaccgcctc aggccacaac gccgccaccg
    54601 gcacgatgga ggtcgtcgcc gcgcagacca tcatcgtcga gccggtgctt cagcgcagct
    54661 tggtgacagt caccttccag gtgcgcccgg tgccgttcac cgaccggtat gagattgtca
    54721 ttgagcagac cttcgagacc ttcgtgcccg cgccggtcct cgtcatgacg ccgccgaagt
    54781 atgatttcaa caatgtgcgc cccggctttg agaccacggt gatttacgag ttgaaaaatc
    54841 acggcctcat caagatattc aatgtggaca tcaccggcac ttacgcgggc ataatgacgt
    54901 tggagcctct gatagacttc atccccgagt tgctgccgca acaggtcgtg caaattcctt
    54961 gccgcatcag actcggtcaa aatctggccg tcgcggaggc gggcggctgc gatgaaagtc
    55021 ttgaggactg gatcgagcgc cgctccaatc caccgcccca gatcaatccg gacgggtatg
    55081 tttttggggg accggcgccc accatcggca acttcatcgg tggtttttgc ccgacgccga
    55141 atctcgcgga cttcatcaat ggattatccg ccatcgcctc cctcggcgcg aatggctgct
    55201 acgcgagcca gggcagcggc aatctcggcg acgcgcttgc cgtggccctc gccgcggctg
    55261 tggtttatga gacactgacc agcattccag gctcggtggc tgatgtggtg atgcaggtgg
    55321 cggccgcgct cggacaatgc gccgccgcgc attttggcgg tggtggcggt ggtggcggag
    55381 gcgggggtgg aggcccttcc agaaacacgg gcaattacgg tgtgggcggc aatggctgct
    55441 ttaccgcgga cacactggtc ctgctggcgg atggccgcca gactccgatc tcaaagatca
    55501 aacccgggga cgaactggcc atcggcatgg attcgcgcga gacggccaaa gtcagccagc
    55561 tttacacgct ggagtcggac gagcttttgc tcatgagcct gcgctccaaa gatcaacccg
    55621 ggaatgaaag ccgcgacttg cgcgtcacag gcgagcactt ggtttggcgc gacggatccg
    55681 gctggactca tgcgcgggat ttgcgtccgg gggactggct gcatgacagg gcgggaggca
    55741 tgatcgagat cacctctgtc aaatccgtgc ccggcaagca caaggtcttc acgctccaac
    55801 tcaagggcgg cagcaccttc tatgcgggcg gggtgctcgt ccaggatcta tgcggggggc
    55861 tttatcttga tccatccgtg ctctccaaac tcgaacctgt gaaggaatga attctcaccg
    55921 aggaaccatc atgaagacct ttctttccat ttttccaggg cctgctgccg ctgtcctgct
    55981 ggcgctgatg tgcgactcgc gcgcgttggc caccacgcag ttccaaggct ggtgctcaaa
    56041 agtgaaaatc cagatcgtcc aggagctggc tcttgagcgc accggattcg aagccgagct
    56101 gactgtcacc aacaacgacg gcgcggacgc catcaccgac ttcctcgcgg agcttacctt
    56161 caccgatccg gccatcaccg agccgggggc gaatcccgac gcggcgaacc tgtttttcgt
    56221 gcgccagcca accctcgaaa acatcaaccg tgtggatggc accggggtca tcgcgccgac
    56281 aaaaaccgcg gtggtgaagt ggttcatcat tcccaccatc ggcgcgggcg ggacaaaccc
    56341 gcagggcagg cgcttcacag tgggctgcaa actttccggc aagttgcaag gcgtggaaat
    56401 cccgcgcgac aacatgttcg cggtgccgga caccatcacc gtgaagccgg agccccaact
    56461 gcgcatcacc tactttcagc cgagggatgt gctgggggat gaccccttca ctgtggatgt
    56521 ggtcgagtcg cccatcccct tcacactcgg cgtgctggtc cacaaccacg gcttcggacc
    56581 cgcgcggaat ctgcgcatca actcccagca gcccaagatc gtcgaaaacc tcaatgggct
    56641 gctgctcgtg gcgcgcctcc tcggcgccag ggtcaatgat tcgccactcg acgaatcgag
    56701 cctgctcgtg gacttcggca ctttggagcc gacgcagacc ggcaaaggag cctgggacat
    56761 gatcaccagc ctcaacggca ccttcaccga tttccgcgcc acctacaccc acgcctccga
    56821 gcttggcggc caggccacct ccatcatcac agctctggac gcccacttca tcgcccgcga
    56881 ggtgctcaac gacgatccag ggcgcgacat catcaaggac ttcctggccg atgtgaatcg
    56941 cgatgcggac gaaatacccg acgcgctgta tgaaacccag gagggcaaca tcctgccggt
    57001 caaccatcag cagaacgccg ccgtcactgg caccctcggc gccaacaatg tgcgggtgga
    57061 gatcgtctcc gctttcgaga actggattta catccgtgtc cccgaccccg gccagaaccg
    57121 caagcccatc agccgcgtcg tgcgcagcga cggcaaggtg ctcaatgcga acaactattg
    57181 gacgcacacc aagtacaccc gcatcggcaa tgtccggctc gactggctga acatcttcga
    57241 caaagtggct gacaacacgg cctacaccta cagcctgact tacgatctcg acagcgtgga
    57301 tgtgacggct ccggtgaccc gcatccgctt ctccggcgag cacacggaga gcggcggcat
    57361 tcaccacatc acgcgggaca cgcagatctt cttcacctcc gaggacgcca gccccgtcgc
    57421 catcgaatac cggatcaacg ccggcaattt cattcccgcg ctgcctttca ccatcaacgc
    57481 ctccggcacc tacaccatca ccttccgcgc cactgacacg gcgggcaatg tggaggagga
    57541 caaagtggct ctcgtctcca tcagcggtgc aggcccctcc atctccagct atgcggtcaa
    57601 tgtcggcaat cttttccttc cgggctcgcc caacgtggtt tccgtgcggc ctggacaatc
    57661 tgaattggaa ttcactgtgg ctccgaatcc tgtgcgcacg gacgggcggg tggacatctt
    57721 ccgcggcgtg gcggcctggc cgcgcatcag cggcgtgccg ccgaccccaa ctccgctgaa
    57781 tgcagccgag ctgaacgtgt cgggcaagtt cgtggacttt tatcgctaca aaatcaacga
    57841 gggctcctgg agtcccgagc gcccggtggc cacgcccatt tctttgagcg gtctcaacgg
    57901 ctcggtcact gttagcgtca tcgcgcgctc gacatacggg gattacctgc ccgaggacga
    57961 tgccctggaa gtggcgtgga ccgtcaaccc cggagcgccg ctgctgaatg tcgcgggtgt
    58021 gccgcccacc cccactccgg accaaaactc cgccacgctg gccttttccg gaggcgctga
    58081 cctcacggac tatcgctgga acctgaacaa caccttttat caagccgagc agccgatcgc
    58141 cacacctctg gagctgacgg gacttgatcc tggcgaccag gtgctcggca ttcgtggcaa
    58201 gcggagcggc atctggcagg aactcaacgc cgccactttc attcattgga catacgatcc
    58261 ggcatatggc tccgacttcg cctcgctgcc gctggtgaag actcaggatt acgaggacat
    58321 cgccgggcag accttgacga atgtctggga cggaaaaaac ggcgcgggtg tgccgcagct
    58381 tcccggagtt tacaccgtgc gcctgaagct caccgacacg ctcggccgcg tctcctatga
    58441 ttcggacctc gtcttcatcg agaagctctc ctccggcgag acccagctcg gcgacgccgg
    58501 ctcgtcccgt ccggacgcgc gcggctcctg ggccgtgtgg cagcggcagg tggacggcgt
    58561 gcccgcgatc cgggcgcgca atctcgtgac ggacactccg gtggatgtga cctcgtcacc
    58621 gctggctcag gaaaagccgc gcaccgacgg tcgctatgcc gcctggcagg gacgcggtga
    58681 aaatggcgtt ttcgatgtct tttatgccga ccttgatgcc gccatgcccg cgccggtcgc
    58741 ggcgaccagc acacccgcgc gggatgagat caatcccgtc atccactggc cgtggatggt
    58801 ctatcagacc cgcccgtccc ccgccaacag cgcgccctgg cagcttgagg cgcggaatct
    58861 ggagactaat gccgtgtttt ttgtggacac caccacgcag gaccagctcg ccccttccat
    58921 ccactcgggc cgcgtggcct ggcaggactg gcgggatgtg gggccgggcg agatttattt
    58981 cgccgacctg gagaccgggg aaaagcgccg catcaccacg cagccagccg gccagtacaa
    59041 tccgacgatt tttggtcaca ccatcgtctg gcaggacaac cgccacatcc agcttgagtt
    59101 gtacaagttc gatctgcgca agggtctcga agagcgtctg accgacaccg cttacaacga
    59161 ggtgaaccct gtgctctgcg gcaactggct cacttacctg gaggactccc tcggctccga
    59221 gaccgaaaac ctgcgcatgc tcgacctcga cagcggtgaa agcgtgccgc tgacccaggc
    59281 cgagggcagg cactcacctg gagcactcgg ggatggttac gccgtctggg cggaggggcc
    59341 tgcgggtgcg caaacggtca aggcatcctt cctgcccagc ctccagccgg tgatgcgcaa
    59401 tcacaacacg gtcgtagtca cagcggagct tgccgcgcgc taccccaccg ctttcgcttt
    59461 gctcgccgcc tggaacgagg ccgccaatgt cgagtccatc acgcgttacg agtccatcgc
    59521 cgtgccgccc gtcactgaaa cggcggcctg ggtggcggac gcggccactg gcgacgactt
    59581 tgccatcacg cccggagact tcctttggat ccgctttgac caggcccgga tgctcgaact
    59641 cggcgaggcg gacgccgcgc cggtggacct cgccgccggg aagaacgtgc tcagctacag
    59701 tgccttcccg gtcggttact ccgcctatga tttcatcaac gccgtcggcg ctgccaacat
    59761 cggcgcgctg cgcatgcttg atcctctggc cggcgtctgg cgcgcggtcg aaatcaagga
    59821 aggcgcgccc gtcggctcca acttcgccat cccccgtgtc accgccctgc tggtggacat
    59881 gaaaaacgcc gtcaatgact ggcagccatg aaacccgaac ttcaacctag cacccggatt
    59941 atgaaaacct tcgtcatcct ctccctgctg gcctccagcg cccttcgcgc cgccactccc
    60001 gagcagctcg acgccatgct ccagaccggc gagaaagtca ccatcattga cctccgccca
    60061 acggatgaat accagctcag ccatattgcc ggagccatca acatccctca ccagctcgtg
    60121 cctggaaagc gcctgcctcc cctgggccgc gtggtcgcct actgcgatgg tcttggctcc
    60181 acctacacag cggaatgcgt ggccgcgctc aatgccaagc ccggcatcca ggccgagggc
    60241 ctggagggtg gctatgccgc gtggaaaacc ttcaccaacg tcacggctga cacccgccgt
    60301 gtcacacccg atcagccgaa aacgatcacc tatgaggagt tgaaagccac caggggcgaa
    60361 ggcgtggtgc tcgtggatgc gcgtaaaccg cagaccgtgg ctggcaagcc ccgcgtcaat
    60421 ctcgccgcct tccggcagca gaatctgccc aaggccacca gtatgaccaa cccctccgcg
    60481 cgtctgcgcg ccatcaaggg caaggcgggt gagttcagca aagccccctc cctttttgtc
    60541 gtcgtggatg acgaccacca gagcgcgctg gccgccgccg agcgcatccg cgccatgggc
    60601 taccagcgcg tcgtcgtcct cgccggcggc gaggacatca tccgccgcga tggcaggccc
    60661 ggcctgggcc gccagggcgg cgccgctccc gtcacactcg atccagaact cgtcccgccg
    60721 cccgcgcagc cgaaatgaga caaccccagc cacacctttt cctcatgaaa caggcatccc
    60781 acttttttct ggtcctcctg ctgggcctgc tccagggctt gaccgccgcg cgcgcgcagg
    60841 tcgccatctc cgggggccag gccggcaatg tggacgccgg acaattcact gtcttttggc
    60901 aggtggatga caccggcagc cggcccggac tcgacctgtt ttcagacgcg gcagccacag
    60961 cctcgctcaa tggcgaactt ggcgtggaat tctttcccct ggagatgaat gacgtcaccg
    61021 tcggccagac gccagcccag cgcgaggcgc ggcggactct ccaggccctg acgaaaagca
    61081 agggcatcat gctcgccaaa gtcacaggcg cccagccggg tgcgacatac tatgcgcgtc
    61141 cgcgctcttt tgacgcggga acgggcctgg ccaacgaggc tgcctccgcg cctttgattg
    61201 agatcaccac gccggagagc actgccttca ttgtcgagtc gcgccaggtg ctggtaagca
    61261 cgggaggctg ctttgcgggc agtcaggggc tggtgatgcg gctcgtgaaa gatggggcgc
    61321 cgtatccttt gttcgccgtg gtgggggatc atgggcagac agacaaggcg ctctttgatg
    61381 tgaaccggct tctaaatgcg gccgggacaa ccaacgagaa cctggcaggc gcgccctcct
    61441 tcgagttgga cttgatcggc ccggcggctc ccttgggagt cttcaatctg gacctgccct
    61501 ttgccggtga gtttgtcgtg gcagccgcga ctgaggtgga tttcaccgcc aatttccccg
    61561 gcctcgccta tttctccatg gagggcacaa gtccgccgct gcaaggcgtg cctttcacct
    61621 tcaacatcac cgcgcgtgat tccggcggca atgtcctgac aacttatgac ggcaccgtgg
    61681 atttgaccac cggcaaccct ggcgacctca tttccggcgc gggagagacc gcgcatttcg
    61741 tcaatggcgt gctcgccggg catcaggttg tccccgcagt gtcaggcccg gtcacattta
    61801 ccgccacgcg cccttgtggc atcgagaccg gcagccaggt cttcaacttc tccccgccga
    61861 acctagacat caccttcagc gcgccgcttt ataccgtcag ccagggagcc atgcaggtca
    61921 tcctcacact aacccgctcg gagacactgc ccgcatcggt catcctccac accaacgacg
    61981 gcacctccca gaccgcgaat ccgcccttcg cagccgcgct ggcgggcacc gattacctgg
    62041 acttggacgg cgcggcgacc caggtgaatt tcgcccagga tgagacgagc aaaatggtca
    62101 cggtgacttt gcatcccaaa actggaaccc gcattcccaa caagcgcttt accgccaccc
    62161 tgcacccgcc agtcgcagag gcggcattga ccggggccct caccacggcg gctatccaca
    62221 tcctggccga ggacacactc gcgccgaagc tgacggtatc caagccctcc gccaccacaa
    62281 ccgcgctaag tcttccccag ccctacaccg tcatgggcag tgccggggac gcgcgcggca
    62341 tagacagggt cgagatcgtc ctgaatggcg agccagccac ctttgccaca ctcgcccccg
    62401 cgcccaagcc cacctcagtg cccttcacgg cggagatact gccccgagaa ggacctaacg
    62461 tcctcgaagt caccgccttt gacctgagcg gcaacagcac caccgtcacg cgcaacttca
    62521 ccttcacccg cagatactgg ctgaccctgc accgcatcgt gcccgaaccg ctcgaggcca
    62581 cgccggaact ggccggagcc ttcgcgctga cggcgagcaa caacgccaat aaatccaccc
    62641 tctccccgcg cgcgggcaca ccgcagctct ccatggtggt ccccggcacc gccttgcaaa
    62701 tcacagccaa tcccgcggcg gggcattcct tcagccactg gacgggtctg ccagccgaat
    62761 ctgttgtgct gggcaatgtg gcacgcttca ccatgcctgc cagcgacgtg ccggatgtca
    62821 ccgccgtctt catcgtgaac cccttcctgg ggcttggcgt caaagccgac tttcttggtt
    62881 tgctgcaccc ttccgacagc acatccatct cgaactccac cgtcggcttc ctgcgcggaa
    62941 cgctcaccac ggctggcggc tccatgagcg gcaaaatcca catggacggc gtcgcgcagt
    63001 cgtttgtcgc cctcgtcttt ggcaatggca atgtcacctt taaagtcggc agggacacct
    63061 tcacagggct tgagtttgcc ggcaggatgc tgacgatgag ctacacgccc ggcgtgctgc
    63121 acttgcaggt gacctccggc gcggaggaga gtgaaggcca ggccgtcccg gccatctaca
    63181 gcgccacagc acccgtgcct ggcgcgctcc tgaaccgctc tgccaaggat ggccagccca
    63241 tcaaccaggg atacttcacc ttcgtcctgc catccaaagc gcaggagccg ccgctgccat
    63301 tgaattccta tccacagggc gcgggatacg gcaccctcat cctttccaac acgggcctgc
    63361 tcaaggttgc aggcacactc gcggacggca ccaaactcgc cgcctcctcg gccctggtag
    63421 ccggcgacgc cagccccctc ttcgcccaaa tcctcacccc cggcaccacc accaaggcgc
    63481 ttggcggcag ccttggcggc acgctggtat ttgaccctct gaatgcggat ggcgatgtca
    63541 gcggcgagga cctgctctgg ctgcgccccg ctgtcaccga gaccgccacg ctggccacgc
    63601 agatttacac tcatggatgg cctgacggca tccgcgtgga cgccaccggt gcgctctatt
    63661 tcgcagcgct caatgtgcag accggccttg atctcccggc ccctgcccct gatggcaacg
    63721 ccatgctctc cttcatcggc ggcaggctcg ccgaaaccat caccaagtcc aatttcaaca
    63781 tcagcggcaa cattttcacc aaaatcccgt ccaccgacag gaccttctcc ctcacactga
    63841 cgccgaagac tgggttcttt agcggcaagt tcaccccaaa ctggccctcc gcctccacca
    63901 cccagccgtc gttcaacggg gtgctgctcc agaaaggcgt gaagaaaggc ggccacggcc
    63961 actccatcag caacgccgtg ggagatgccg atcccgaaag cagcgcggtg gaactgctgc
    64021 gcaggccgtg agacatgcct cttgtcatca gattttgccc ggggcggcaa aagcggctgc
    64081 gcttgggcgg ttgggatgcg tcgg
//
LOCUS       DAOMYJ010000003        53044 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Prosthecobacter sp. isolate AMR_MDS_5629
            NODE_244_length_53044_cov_25.417615, whole genome shotgun sequence.
ACCESSION   DAOMYJ010000003 DAOMYJ010000000
VERSION     DAOMYJ010000003.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867850
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prosthecobacter sp. (wastewater metagenome)
  ORGANISM  Prosthecobacter sp.
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Verrucomicrobiaceae; Prosthecobacter.
REFERENCE   1  (bases 1 to 53044)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 53044)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.30
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:34:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,742
            CDSs (total)                      :: 4,684
            Genes (coding)                    :: 4,676
            CDSs (with protein)               :: 4,676
            Genes (RNA)                       :: 58
            tRNAs                             :: 55
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..53044
                     /organism="Prosthecobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_244_length_53044_cov_25.417615"
                     /isolate="AMR_MDS_5629"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1965333"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..445
                     /locus_tag="PLP58_00660"
     CDS             <1..445
                     /locus_tag="PLP58_00660"
                     /inference="COORDINATES: protein motif:HMM:NF015823.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PqiC family protein"
                     /protein_id="HRJ07061.1"
                     /translation="PALAVARPSLPSYLDRQQLVTRGAGGEVRVLDNHLWSEPLDSGI
                     ARVLAANLSRLTGSTAILPVGNFITLDYSALVEMRVERFDPDPSGNLVLECAWKKQPV
                     SGADTPFKSFRAEVPVDPSKAPMTGRIAAMNEALARLAREMARGL"
     gene            516..1886
                     /locus_tag="PLP58_00665"
     CDS             516..1886
                     /locus_tag="PLP58_00665"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HRJ07062.1"
                     /translation="MRFRNLTRRLDIGANSYLFESGKHRVLVDAGMDPKETGLKALPD
                     MGPLPHRAANAILVSHAHHDHIGALPVAQRAHPDTDVFMTEPTGEVGTAMLHNSVNVM
                     TSQREEKAITEYPLFTHRELDENRARYVYRDLDRPFELPDTDITASFHDAGHIIGSAG
                     ITIRQNGGTLFYTGDVNFEPQTIARAADFPVDGIDVLVIETTRGNYQRPADYSRKAEK
                     ERLAALIRETHEANGSVLIPVFALGKTQEVMLMMHELHEEGLIPEMPLHIGGLGTKIT
                     VLYDHYTDRTRRNYAGFRLLEDVEMLVPPRRKKGPARRHIEYEPRAIYALSSGMMTEG
                     TTSNQFAARFIDNARNAVAFVGYTDVETPGYRLRNARPGEKIRLAPDQHEVPVNARVA
                     SFDLSAHASREAIAEYVEKVRPKKVLMVHGEPPAQDWFAARFAETLGRETEVIRPEPH
                     MAIDLW"
     gene            complement(1913..3094)
                     /locus_tag="PLP58_00670"
     CDS             complement(1913..3094)
                     /locus_tag="PLP58_00670"
                     /inference="COORDINATES: protein motif:HMM:NF015679.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HRJ07063.1"
                     /translation="MSMPAPALQVLASLSLTRQVPSSDPAALTLPPNTPTAPVATPSS
                     PPPKPPVEQPPAAVEVAITPPKPAPAPKPKPWQWTPLRDSVEEYFRTHLPDLGMLPVI
                     QLAVMYNDEEWISRLVAGGVNPDEATPGGDTPLCAAIRLGATGAVRALLYGGADPRLM
                     NFEKQPPITLASLRRGSDILHALIAAGADPNTRFNHPVAKSVLERVTIKDLRHYLEVD
                     RGLTPLIACASRGDVEGAAELLKAGAKASLCTTRYKRYPINFAATQGYLFLMRIILGR
                     QPESEPRILVTVDLGKQRAWVTKEGQVIDSCPVSTGRAGYATPAGRYVITDKHRSWTS
                     TLYHVSMPFFMRLNCGAIGLHSGYVTGRPASHGCIRLPYDKAKKFFEITRVGDEVQII
                     Q"
     gene            3295..4701
                     /locus_tag="PLP58_00675"
     CDS             3295..4701
                     /locus_tag="PLP58_00675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009960471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mandelate racemase/muconate lactonizing enzyme
                     family protein"
                     /protein_id="HRJ07064.1"
                     /translation="MKNSKSTDIKVTSASVFFLPVTMRVPLKFGPETVTNTVCLRVKV
                     GVEDRQGRHAEGWGETPLSVSWVWPSTLSVAERAARMDSFSILLAQRLVASGLSGHPM
                     EIGHDFIHGPLARTLAEANAEAGGPEMPYLGSLVAFSAFDIAVHDAFGNLLGVDVYST
                     YGPDFMSRDLSAYLEPEAGSGLSFVGLYPQDFLAREAPSKLPVWHLVGGVDALEEADL
                     TGGEPQDGHPLLLADWIRQDGLKCLKVKLRGNDAAWDYERMVRAGRIGLPLGVRWLSS
                     DFNCTVRDPAYVNEINDRLLRDEPEIYARTLYVEQPFPHDLEANQIDVRSVSARKPLF
                     LDESAHDWEFVRLGRRLGWSGVALKTCKTQTGALLSLCWARAHGMPLMVQDLTNPMLA
                     IIPHVRLAAHAGTIQGVECNAMQFYPEASVIEERVHPGLYRRREGMVDFSTLRGPGFG
                     CRVEEIARVLPEPAAVAG"
     gene            complement(4702..5484)
                     /gene="folE2"
                     /locus_tag="PLP58_00680"
     CDS             complement(4702..5484)
                     /gene="folE2"
                     /locus_tag="PLP58_00680"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012419153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003933 - GTP cyclohydrolase activity
                     [Evidence IEA];
                     GO_process: GO:0035998 - 7,8-dihydroneopterin
                     3'-triphosphate biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase FolE2"
                     /protein_id="HRJ07065.1"
                     /translation="MPALKDTQNERDDRRLPIDRVGIKNLRFPLCVRDRDANPQHTVG
                     TVSMAVDLPHHFKGTHMSRFVEVLHAHGRELTVADVAAIPQELQKRLAADKAHLEIRF
                     PWFRVKKAPVTQAEGMLDYGIIFEVNADGAELDFVVTVEAPVTTLCPCSKAISRHGAH
                     NQRGLVTFSVRFHQPVWIEEMIEMVESAASSGLYSLLKRPDEKHVTEAAYENPVFVED
                     LVRNVALKARAHPRVRWFRVEAENFESIHNHNAWAVIESGPA"
     gene            complement(5566..5805)
                     /gene="rpsR"
                     /locus_tag="PLP58_00685"
     CDS             complement(5566..5805)
                     /gene="rpsR"
                     /locus_tag="PLP58_00685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009964263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HRJ07066.1"
                     /translation="MQPKTEERLISLRRNNRRMPRRRMDIPVDKLTYTNPELLKRFLT
                     ETGKLIPRRVTGLPAWLHRKVTREVKRARSVNLLP"
     gene            complement(5818..5991)
                     /gene="rpmG"
                     /locus_tag="PLP58_00690"
     CDS             complement(5818..5991)
                     /gene="rpmG"
                     /locus_tag="PLP58_00690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012419155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HRJ07067.1"
                     /translation="MPREIIILECTEAKAEGKPTSRYVTTRNKKSVRTPGRLEKVKFN
                     HFLKRRTLHREIR"
     gene            complement(6015..7061)
                     /locus_tag="PLP58_00695"
     CDS             complement(6015..7061)
                     /locus_tag="PLP58_00695"
                     /inference="COORDINATES: protein motif:HMM:NF013427.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TraR/DksA C4-type zinc finger protein"
                     /protein_id="HRJ07068.1"
                     /translation="MPAKKKPAPKAPARTAKKPAVKASAKPAKKAPAKPVKKVSARAP
                     AKPAKKAAARPAPKKSPPVKKSPSKPAAKKPAPKAAKTAAPAKSKKPAVSKTPAAKKP
                     AAPVKKAPPAAKAPVKKTPPAKEAKPAAAKPVQNKETTAKKPKGLTNGAKIITVKRDT
                     SSIDDGITLVEPPKKPVLPAAFLKKQKQRLVELRDAYMNAAEGVAAESLRNHESGDSS
                     AFGMHQADAGSDAYDRDFALSLLAKEQDAIYEINEALNRIARGTYGICEMSGETIPEE
                     RLEALPFTRFTVNCQARIERDQRGGRWTRPVRSLFGLDEAADDSEDSDEDDAVTPAKS
                     KSNENNESLDFGKE"
     gene            complement(7224..7631)
                     /locus_tag="PLP58_00700"
     CDS             complement(7224..7631)
                     /locus_tag="PLP58_00700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009958735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HRJ07069.1"
                     /translation="MLNILRLSFLSGCSDLGLLVLRVSLGFTMLLLHGWKKFENFSEI
                     APGFLSLFGLPSHISLGLAVFAEVVCSVLLIVGLFTRFAALNLAVTMAVAFFIAHGGA
                     FAGDKPGELAFVYLTGYLALVFTGAGKYSADGR"
     gene            complement(7684..8238)
                     /locus_tag="PLP58_00705"
     CDS             complement(7684..8238)
                     /locus_tag="PLP58_00705"
                     /inference="COORDINATES: protein motif:HMM:NF023393.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein O N-terminal
                     domain-containing protein"
                     /protein_id="HRJ07070.1"
                     /translation="MEKTEAILIGRTRWSETSLIIHWCSPGLGLFRTIAKGALRPKSP
                     FQGVLDLFVSAEIRFVPAKKSDLHTLAEAHCLNPRLGLRASYGRVLAATYFTRLLTLA
                     VEPHSPLPALHELLDRALDFLGSHDPSRAMLERFELRVAEDLGLIGEKGAPGSEAARI
                     LEDHLHKRLPAQRAQVLAWCADHP"
     gene            complement(8251..9282)
                     /locus_tag="PLP58_00710"
     CDS             complement(8251..9282)
                     /locus_tag="PLP58_00710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ07071.1"
                     /translation="MWKKYWDQWGGRALAASLAVHALLLLSAAWIVVSQVQEKQVDFL
                     SGGGTKQGAEAAAALQHRVQQKKKPWLKKPVPMRKLAVANSINSLVLPDDAPDLLDIP
                     EASSLLGGGKLSGGMGLAGSGGGFGKGMGIGGASGVTFQPFSLFGMQVKAKKLAAVLD
                     VSGSMAPHLPRVITEVDKVAKGSVVVLYIGCGLEPPPPRGLDGKNVIATSTPDFDKFW
                     RLLWQTGGATLAEARSYRPPAGEPMPSQDLYNLLKKRPRTYFLYNVGISYAWLALLSD
                     QVRDADGLYWFSDFQDNVDFRQVRTVRENLEQRKQRLYAHAYFQGGSFDLIKSQLVEP
                     TQGDYVLEQ"
     gene            10034..10297
                     /locus_tag="PLP58_00715"
     CDS             10034..10297
                     /locus_tag="PLP58_00715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ07072.1"
                     /translation="MPPALSAVGRRLKSSSGFSGGMERKAASPARRNREESDSNARLF
                     PDVGRRMLSLRPLNGQQIHKEDSVLGGESRFPQPGTKTATYEE"
     gene            10294..11331
                     /locus_tag="PLP58_00720"
     CDS             10294..11331
                     /locus_tag="PLP58_00720"
                     /inference="COORDINATES: protein motif:HMM:NF012264.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HRJ07073.1"
                     /translation="MIKTAFKTEWWQGVEVVPEDAKEPKAHKQRKKKMAKSGGLTKMS
                     NSVLGPDLDTDSRYSQMEENFSLYFGLAVQLYEATLVSDDTPFDRFADGKKDALNKEQ
                     KEGLDLFFGKAKCANCHGGPELTKATVGHIKKERLERMAMGDGGMAVYDNGFYNIGVR
                     PSFEDLGLGVDDPFGYPLSESRVARIYGKDGFKALIGRDPNLSVGQQERVAADGAFKT
                     PGLRNIELTAPYFHNGGTRTLLEVVEFYNRGGDFHDKNIQDLDPDVERLHLSQKEKEA
                     LVAFLHSLTDERVRYDRAPFDHPQFFIPNGHIGNENTVSNEGTGRAKDILIEVPAVGA
                     KGGAARPNFLE"
     gene            11526..12209
                     /locus_tag="PLP58_00725"
     CDS             11526..12209
                     /locus_tag="PLP58_00725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ07074.1"
                     /translation="MLVLCACLGLAVGRAGAAGTIFWSSQFQDLLFDSQGQELDHSFA
                     FEIGSFGSFIPTYANMSDWEANWKVFDRAVDGNGWFADDQFFFGAADHNDLGGSDSPA
                     ADPLDVFLAGEKAYFWVFNSKALAAGSEWALVTDSDFFSNTGDSWIFEDSSDPLYFGS
                     DWMLEDADEIIVGGLLNVQGDGEFSTLPGAFTLQTAAVPEPGSALLFWLSCSTLLLRR
                     VSKRLGILA"
     gene            12460..46941
                     /locus_tag="PLP58_00730"
     CDS             12460..46941
                     /locus_tag="PLP58_00730"
                     /inference="COORDINATES: protein motif:HMM:NF024777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SdrD B-like domain-containing protein"
                     /protein_id="HRJ07075.1"
                     /translation="MIHVLCAVRLEAHNLNHRINYVAFDHETMEMIKARAKAGQQLLR
                     ANDIVGLVMKATPDVGTPTGAGGYSTFFIPVGGQVVGAHYGFLNKNGEFVITPMKGQS
                     ILALGDGSSGSKSSPQLVGLQLGPNITGQTSLVVDNSGAHRGTMAGLYSDTGIFYSTD
                     PATVWMSWATGGGMDGNPATLDNLMVNNSGDVVTPTTRWDAEQLLAWGSKNPPALIDS
                     QDQRGNAPWGMASAVAGPESGYAWHFNKSIWDGMSANPNRMKLSVSNMGPWKRIRYPG
                     SMAAKDVPGRKGSDLGYVGVNAADIGHDLNTFNPLPPTTSWTDSTSPKAIRMAWGNLE
                     LFRPEYCRVRLKILKGPGESGSPFDSDGFLLSYADTFGGDAGGEYNNKDHLWRYYEPT
                     RTDLAGKPFIAKHASKPIVASGENFHYDITVVNFGFNTLTNVIVEDLLPSGVSYVSAQ
                     PPLTSTSSPLTWKIPSMAPQTVKTFKLNVRATGSGIQVNTASTRSDQTPKTSTLEIID
                     IISQALLYPDKAVTPSTAQPGELVTYTLVIENTGTAASATPMVITEMLPDGFVYDSLV
                     SARVNNAPVSNGSLSVNSANSARPVFNLSQAINADGKLEITFRARISQGQPAGTYTNS
                     YIYAYSGKVMSTGALAPVTVGGARLGGKVFRDWNGDGQMIAGEEGMPGLSVQLWNAAG
                     TTLIGSRTTDSSGGYVFAGVASPATYQVRVEGVPTGYTATYDLDGVSSASRASVPLAA
                     GQERMDVDFGYQPGGAAGVGGRVFNDTAKDGKYQAGTDSGIASVTVRLHHDRNQDGKL
                     DAGDLLLSTATSGAEGVYNFTGLAPGLSYLVQVDAEDADIPAALGGQPVRITTPGVLA
                     VPALNSQVTGKDFGFWKEQPSSLGGRVYLDNNGNAVFDAGDTPVTGIPVRIFHDADGD
                     GTARQSELVGVAPSDGGGRYRLDDLPGGDFIVRLGTESISMPPGYRALSEEHLLVLET
                     GASRLDLDFMLAQLLSKMANRSSAVQGDQIVYTLIPNYQGDGALENLTIQDVLPAGTT
                     FVSAGQGGALGSFVSQPGSPGQAGNPVVQVANGTYTGNGSDNRNITSAGFQPDLVIIK
                     GASSVAPAFRTSTMSGDASKLATANSLASNIIQSLLSNGFQLGTNAAVNSNGMRYDWV
                     AFRASAGVLSVGSYTGNGSSASRLINNPGFSPEAVFVMPANSSAVVMKTATMMQNESF
                     LFTGGSAATNLITGFESTGFRVSTGSNASSTVYHYVAWKAMDGITSTGSFTGNGVDDR
                     SITDPGFRPEFVLMRSASSSAAVMRPASLTSDLSLFGSATASAANNIQSLLDQGFQVG
                     TDARVNASGSEIHWAAFRAAAATPLTTTALNVSKTLCKNSDLVTLTMTLTSNSTLSGI
                     LPGSPAVNTSGSASAVLVNGPTPSTLNLSAGGSGSFSWTYQVDAGAGSGDVSFTCAAS
                     SSGYAFAPGTSPSLNVSSSGTGQSVTWNLGSTSAAAPGSLVQNLNLYAFQGDNKTGFY
                     RYDAGTGLWQARASAPASVYWGGALASDGTHLYALRGNDQSTLWRYDPATNAWTTRAS
                     APAAIKEGGALVYLAPHFYALRGKSTATFYRYTPSTNTWSTMASLPAAVNAGGALTTD
                     GTFVYAVRGDGKKEFYRYNPSNNTWTARASVPGGVGKGGALAFDGTHIYALRGGDYRE
                     FYRYDRTANTWTRRADTPSTVNDGSALVFAGSTIYALRGDNAKDFWRYNPSSNTWAAM
                     TSLPVNVNMGGAIVDYGAQTPTEIGVGIQVSPSLLAHGGTARVTMTLSSSEGQINNVT
                     PPALSVQGTNGASATLLSGPQPASATVPSGGSASFVWTYQISAGTNPGQVVFASSAFN
                     SGGKPFPAATSNGVIVTRPLRLTVTVTDPAGLDSVDNVATIQTGEFAGSYVVANLTSG
                     SKEDRLLSVDQGRVTDLGATGTTRVRAITLAANGGLYAAARDSTIGTGADLFGLITPG
                     GSASTLRQWANTVVGFSSEWTNDGSSWRAIQALGEPDTFSAGDLPTAWSPATNTSNPE
                     YLHLGYATAVYATLIRVRETYLGQFVTGIELVEPDGTRHSIPIPPDGTVVPGYFEVSF
                     PATSYLVSSVVIHTAKSGYEEIDAVELVGMPAGSGSFIPLGRAASPTEPLEGPLGNFT
                     TLTITAMDTDPQTGMIYAIHRREDATMANTLLDVLFILNPETGLHVEDAFGPGVDYVA
                     VATNTLPVPLYDIDDITFHPQTGELYAIANDSASGVSDQDRLVVINIQTGAVTDIGRV
                     TIKATGEGIHDAGGLHFDARGVLRASTGIGGSAVTNNTLWVLDPETAEAVREVTFTAA
                     YPAFTDYQALASVSVIRGIPSTPSQTVRTALSGSIGDLVFADLNANGVRDSGEGGLSG
                     VRVTVTNGSITRSTVTDGLGGYRVLGLNANGGQPWTVSVDLATLPEDWVATTPPVLQR
                     VLPANGSAINDADFGFIPSDPNGGSIRGLVWMDTTQDGAAGEGESPLAGVSVRLYRDL
                     NDNGVVDSGDLLMRERVTDAEGGYRFSGLAPAKYLVDVVEPSLPPGVVLVSGPPSLSL
                     TTGLHRVTLAAAQHLAGVDFGHNHSGVIAGMVFYDTNHDGMRTPNGADGIAGNNDDEP
                     GIADTGVSVYEDENGNGELDATERVVAATVTNAQGQYQLSGLPPGRYVVRVEEQHIPA
                     PATSPNAGRFDTMLPTTGEVVAVDLAAGGALPGADFGFGELALIEGHVFHDVNSDGLR
                     SPDEPGLPGVMVTLSGTRAAGGALSLGKTSAQDGSYAFLAPPGVFTLAHAVNHPAIPA
                     ALTRVTTPDSYFMELHGGQEIVDMDFGRDHAGMAHGRVFADVNGNGVRDANESGIANV
                     LVELYDAAGITPRDSRVTGMDGAFRFDGLENATHRLKVRADSLPPGYSVMPTADPAAP
                     LDGEAPLTISNGGTVGNLDFGYRIPTPTFTLSGRVWDDGGQGGGVAGDGAINGSEPGL
                     AGVGVQALVDSTGDGVVDETLGTVTNGAGNYSISGIAKDARVTLRVVSSPLLAQGFAQ
                     TADPDAVLDHQTEIASLAANTLGRNFGYRKVPSSLAGRVVSGGNGDGIAQPGETGRPG
                     VTVRLAHEGPDGIPGTNDDVIQTTVTDAAGAYLFTGLPPGYYLVSHVPADGWQPWVDA
                     DGGPPDLIGVELEAGQTLDGQDFEAALPGIGDLVWNDANDNGLFDSGESGVSGVEVRL
                     FHAGADHAIGGTGENADTLAATTTTDNAGLYSFTGMAPGYYFISLSPAQTLPLAARNP
                     VNADNQVNNDNNSLQPGGAGTEIRSPVIEIAPGTEPVSDGDTDPHADLTVDFGLRPCA
                     VITLGPATLPAPPVGVAYSQTLTAGGGRTPRVFEVAAGALPAGLVLNAASGVISGVPV
                     SVAVASFTIRVMDADGCAATRAYSLTPVCGAMSLTTAGLPPGTAGQSYLQSLEVDGGK
                     TPFQFDVSSGSLPAGLALNSGTGVISGTPTAGNGAGTAVTLRVTDAYGCQAAREYTLK
                     ICPVITVNPATLSVPTVGSPYSQTLTANGGVAPHVYAISSGALPAGLALNSSTGVVSG
                     TPTQTTSRTFTVSATDANGCQGARSYTLTPACAPITLTPANAAVANVGTAYSQAFTAS
                     GGIAPHTWSVSGGTLPAGLVLNSSTGVISGTPAAANGAGVTVTLRATDSALCQGALNL
                     VFKVCPQISLAPATLASGTLQVGMSQTVSASGGAAPYTHSVSAGTLPPGLALNSATGQ
                     ITGSPTQTGTYSFTIMSADANGCQGTRAHTMVISNPTFDFGDFSLFGSASSIVSNDLM
                     LGDFVDAEEFAATNLAATGDDITNINDEDGVTLQSILVRGQAGVTVTVKLKNTSPGPA
                     YLNGWVDFNNNGVLSENERIISNVLVENGVTQDYRSFFFDVPSNAALGVVGARFRLTS
                     ASSPGSTGAGGFGEVEDYTTTIVAPSTNFRDYFYAIRIAGTQHYLDEISVFNPNSATP
                     TVSVVPGILDLNVASPGFNPAATNAIMNGLALDWLNRRFYWLATSSGSTGYNATLYTA
                     AYDNVSKTWSYQAVSGSALSNIPFNTGMPTSASAGSGAFPRAAFYDGEYYAGGQLNNN
                     LAIWRLNSSGLGLKTPAISDYPDFFHLPLTFNGGDFVIRPQDALLVTSTVISSSNNLL
                     TQFLADGFNPSGPAAAVVDINPQIPYGTHSSIQIAGVGGVTRMYALGSQGSTVFRIDQ
                     YDTATPAAVRVGPLPNATYADLSEGISTSVTSLGVKGIIYHDTNGLSDGRLNGTGTNA
                     GGALFALLVDLSGNLVDAFPVKSDGTFILGGASSNTTYRVVLSTTYGSLGATAPPAAL
                     PAGWLNVGEFLGSGPGSDGTVDGRLTVSIGNSGLINATFGISRTLSVGDSVWNDANNN
                     GSRDPGENGVSGVTVQLWSPGSDNAIGGTGSAADQLLAASTTNVSGLYSFTGLASGRY
                     FVTVTPPPLFSLASSLSAGDDGVDGDNNGSQPGGWGTLIHSPVVLLADGTEPGNLSSG
                     GGFFDNTIDFGVLPALDFGDHSAFGNASSVVVPGLRLGALIDAEPAATTNATATGDDI
                     TGLDDEDGVTLPASVAPGVSSSMTVNVTNTSGAAAYLNVWIDWNQNGVLTDAGEQVAG
                     NILVSNGTSNSNLTVNFTVPASAGFGPAGVRARLTSTMSPGPAGASGNGEVEDHVLNV
                     TCPAITLIPASTANGTVGLAYSQALHASGGTTPYSYAITNGTLPAGLSLDGSTGVISG
                     TPAVANGAGAAVTIRATDANNCQGSRVYTMIVCPVITVNPASLANGTVNAAYSQAVGA
                     AGGAASYTYSLASGSLPAGLSLNAGTGVISGTPASTSAASFVIRATDANGCAGTRSYT
                     VTPACPTITVNPVSLADGAAGVAYSQTVSATGGIAPYSFAVSAGSLPAGLSLNATTGS
                     ITGTPTTGNAAGTSVTIRATDANGCQATRAYSIKICPVISVNPASLSNPTLGAAYSQT
                     VSASGGVAPYVYAISGGALPSGLSLNTSTGAITGTTSSTASASFTLRATDAHGCQGTR
                     VYTLTPSCPVFTLNPATLPDATQGQAYSQQITVSGANGSVSYAVSAGSLPDGLTLNSG
                     TGLLSGTLSGSGVFSFTVRATDSLGCQGTRNYTINAACAVSSILPASLPHGLPNQPYE
                     QQLIADTSSGLRGEYYTGRNFETLTLVRYDTAVNFDWGNGSPHASLPSDNFSARWTGH
                     VVPSVTGSYIFRTTSDDGVRLWVNGVLVIDQWKDQGPTSYAATVALTAGQPVSVRMDY
                     YENGGGAVARLEWQGPTFSLKPLTEWTTNTWNVVDGSLPQGIELNAATGLLSGILGTI
                     NTSSFTVRVTDWRGCSGTKTYSIQAECPAIAITPETLPPATVGAPYSQALAASPPFGL
                     KGEYYAGMNFESLVLTRQDAAVHFEWGEGSPHASVPVNQFSARWSGFITPSTTGSHTF
                     RVRVDDGFRLWVNNNLIINTWKDQAPAVYTATVNLTAGVTIPVRAEYYENGGGATAQL
                     EWSGPGFSNQPITQWTGYTWSLANGTLPAGLTLNPGTGVISGTPTTTSSGTFIVRATD
                     PNGCSATRSYTLGPACPPLTILPETLADPYLGVAHSRQLQADGATTAMTWSVTSGALP
                     AGLSLSSAGLLTGTPAQTGAFSFTVRATDGGACSATRTYSGATRSMGIGNQVWADMNN
                     DGVRQSSESGIPGLRLELWSPGANGVRENGGGDDFKVTADVFTDASGQYQFPLLTPGV
                     YYIRIPNPPAYYPAVSANGVALDNGVNNDSNGTQPGGSGTAVVSPLITLSPGAEPGVA
                     VDGDDTDTDSTVDFGFANPDVCYAANLFDNPSFEFQQLPNSTGTPATVLGYNGTGTGL
                     GTGINAFQWVGGINSASGLGEPVQRVQLSAGSAGARVSWMESLKSRHGRRHLLLQGTN
                     ATASLRAAGGGDWSTVLQAGREYQLSVWAANASDATASLLWNVTANASIFQVITGPTP
                     GLYQNYTVPQAEMTASLPGQQQCCGNSFTGTSMASFAPADYNGWSEAVENGAQPVWRQ
                     FTWRFRVTPTATATQINTAGFGIGAGSGSGPVVVDYVSLCQVDASNTLTLGNLVWNDL
                     NNNGARDGAELGVGGVSVRLFTTTNNVAGDGDDVLLATTTTSVAGAYNFTNLAPGKYV
                     IRFTPPAHLPLTGGVPATTDNHVNNQNKGSQPGGPGTDLISPVIILALGAGPVNDGDT
                     DPDTNLTLDFGLWSGFTLGNQIWADTNSDGIFQSGSESGIAGVTVQLMDETAASVLAT
                     TTTNISGIYSFTRHQAGKYRIRIPAPPASFPLITAVNVLLDNGVDHDSNGSQPGGVGT
                     AVLSPVITLAAATEPGSSGTTNIDNTLDFGFRACPAIAISPALLTAARQFSAYSVNLS
                     ATGGLAPYTWTVSTGALPDGLSLGAGGLLSGTLGAGAVPGLYNFTARAVDANGCQVER
                     ACSLTMQPPLIGISPPALPAGAQYAPYSQQLSASGGSAPYSWSVSPALPSGAVAWWIA
                     ENGAGDALGSHHGLGLNGLGYTNGPVGRAFNFDGVDDVAQVEDAAALKPAQITLEAWV
                     NPAASGLPANGVILAKSAGSNGYGLGLNSGADNLAFWINDRAGNSITAALTPGVWSHV
                     TATYDGTAMRLYVNGGLVASSAYSTTITHSSEPLRIGNLGGGAAAWKGGIDEAALYNR
                     ALTAQEIAARYLGTQMGNQGMPEGLALDPATGLLSGTPTGVPDTHHFVARASDANGSP
                     GVRAYALEIICPSISISPGSLASTEKGAAYSAELNAAGGTGPYAWSLFSGSLPVGVTL
                     GTAGMISGTPGALGTFNFTVLARDANNCSATRVYTLTVECPVIELAGDALPEARQFEA
                     YTPSALGVTGGEAPHSFAIVSGALPAGMSLSAEGVISGTPSAVPGVYSFTVRATDAAD
                     CAGTREHQITVGCPVIAISPSTLPQAARFAAYSQALAASNGNGGYAWTVSAGALPDGL
                     AMSGSGVISGTPTAVPGDYGFTARVVDAQGCAATRAYTLTIGCPVIAITPAMLPDGTA
                     TAAYSQALTATGGGAPYTWSVEGGSLPAGITLSPGGVLSGTTDSVGTFNFTARALDAQ
                     NCAATRSYSLVIGCPVIAITPESVPDATTGAAYSQTLTATGGVGSITWSMVSGSLPAG
                     VTLSAAGVVSGTPSGAPGDYNFTARATDANNCQATRAYVLKLVCPAITVTNASLPNGT
                     TSANYTHSLSAEGGAGPYTWTLPSGSLPAGLTLNGEGVISGVPTAAGTASFTVQARDA
                     FNCTGTREITLTVECALVAVSTNSVPAGYRGVAYSQALAASGGTAPYGWVLESGALPA
                     GVTLGSNGTLSGTPSATGDFSFTVRATDAHGCSGTRALALSIGGLGLGGLVWNDLDQN
                     GVRDDGEPGIGSVTLQLFRSENSVIGDGDDTLADTTTTDAGGSYGFSGLAPGSYYARI
                     QNPPAGLPFSSGPQVAADNGVNHDNNGLQPGGSGTAVASPLIQLGVGREPGDQAEGAD
                     VDGTVDFAFRAVPAPDAVLLEYNLNASSGGLPPAPSTKNPWIVNSAKLQIEDGVNGLD
                     NLTETSNNGPILPGELSRRVRGWLEDADPALEEPRTSLNAARDTAWVRFGMGAGVTSG
                     GIDSLHLDMRRVDALAPTQGVAYLTWNEGGTFHTAVSDIFEVATQGAWQSVTVDWTSF
                     LGGATALPSGAQLAGKSFLLEIHLHGGDESGFVDLDNVILAGTADGGTPTLALGDRVW
                     QDLNGDGLQNAGEPGMPGMIVQLWRAGADNLPDTGDDVLHASTVTDALGYYLFSELAP
                     GKYFARVPTPDDEWPMISPGSSNDDGVDNDSNGIQPGGPGTAVHTPVIDLALGREPGD
                     QPDGGNQDLTVDIGFSASLSLGNLVWGDNNNNGLVDAGEGGVAGAQVELFRSTDTVVN
                     NGDDVKVGATQVTDSSGAYLFSDLGGGRYYVKVTPPVTHTRRSGTSFNTDNGVDNDNN
                     GITQAGAGAPVYSMMVNLSALTEPGNLLAPFGGNADLTIDFGLRPVFLSVGNLVFKDT
                     NGNNVHDAGEGVGGVRVELLNGDGVFLSSTTTSNASSNLGGYQFSNVLPGQYYVRIPA
                     GEFGAAKPLQNSLSISPASPVDDGLDDNHANGDSGVDSASPALTGISSPRLTLAEGAL
                     PTAATGETGFRAHLDDSADANGNMTVDFGFRAAGPSAEGCYHFAFNDAVRDPDGILPA
                     ASAWQPAQPYDFQYSAGPAHVDAFDLKFDAAVSRIVLDATFTQLSGRRLDALAMMLST
                     TGDPANANHAILYLDAHDRAAPKMAVYRYDPALGLQSWQNSANLLASSGNATGVDVMQ
                     NKVTETGAQARFQFVLDISRVNNAANWAALGVSALTWEGILMGGNAGLGLWTLDLDGA
                     PAYNAGGALTAFPHTSSAATEGGFAPSAGLDIITQPCPSSPWVSIGNLIFSDTNFDGV
                     KQSNESGITGATVQLFSPGADGAIGGEGVNADTQVGQAIVTTSNGAYNFTNLIPGKYY
                     VRVMPPAGFPLTGGGPVPLDNGVNNDNNGHQPGGPGTFAYSPVIDLQPGQEPAVAVDG
                     DGTNGDATIDFGFWSGFSVGDLVWHDINNNGQRDTGEPGVAGIFVELMDPGADGLMGG
                     AGANADTVVATTTTNSSGAYAFRVYQGGKRYARITPTAALSLASDAAVQLDNGVNNDS
                     NGVQTGLAGSPIVSMVFELMPRREPGTSGSTNNELTIDFGILGCPVINISPTALPLVT
                     HQTSYSQTLNAEGGKAPYTWSVVAGTLPQGLTLTSGGLLGGVTTALPGNYSFTIRARD
                     LSGCESTRSYTLTVVCPTLAITPSALPDGTQGAAFSQTLTATGGTGPYSWAQTSGTLP
                     AGVTLSGAGVLSGTPQAPGVYNFTVRATDSLTCQVSRAYAWTVQCPPLSISPATLPAA
                     TLDAAYTAQTLTASGGTAPYAWTVSAGALPQGMGLAANGTLTGTPSGAPGAYNFTARA
                     TDSHGCAVTRAYVLTVNCGVISIAPASLPEAVQSAVYAAQTFTAAGGTGPYTWSISAG
                     SLPAGITLNADGLLSGTPTAAPGAYSFTLRVADAYNCQATRAYALQVLCPVISITPAS
                     LASAVQFDAYHLPLSASGGQGAYAWTVSAGALPNGVTLSSAGLLSGTPQETGVFSFTA
                     RATDGQDCSATRAYSLTVLCPVITITPAALEDAPAHVAYSRTFTASGGLAPYTWDIAS
                     GALPQGMTLSTGGVLGGAPSSAPGVYEFTVRAKDAAEACSATRVVSLTVSCSVVQISP
                     ASLANATVGASYSVNLSSTLGSAPFSYSVVSGSLPPGLTLSSSGLLSGTPTQAGTAGF
                     TIESRDTFDCSGTRAYKLTSVCPVISITPSTPPPAYHSHAYTQQLTASGGTGPYTWAL
                     VSGSLPAGLSFSAAGVISGTPTAFGTSIFNVRATDAYNCQSTQVITLLVKGLAIGDTV
                     YDDANFNGQRDPGESGVPGVTVQLWDPGADNAIGGTGANSDSHISSTVTDAQGRHTFT
                     NLLPGRYYARVLPPAHLPMRGGNPVSLDNGVDNDNNAALQPGGPGTAIFSPVIELAIG
                     AEPLAGDGDPDTDMTVDFGLYRGLKLGNLVWEDSNDNGLRDEGEPGINGVTVQLWSTG
                     ADGRIGGVDDTLLGTTSSAGGGAYLFEHLTPGACYARIPAPPQSHPLVSKVANLRDDG
                     VDLDNNGHQISGGAIYSPVITLTSAGEPGNSGSTYEELTVDFGLVNVTPSAYVSATHD
                     DGIHAFDPGTGAFAGVFHHPFGDSHNQGDGNPFDVPYDIETGPDGHFYVAHFGAGNVR
                     RISLAGADMGAALNTAAANVTLVKRFAIGPDGNFYVVDINTSRMVRFHGPLSDTPGQP
                     LDPAPHTFLQQPGIEDINFGPDGNLYLVIQDGFNREVRRHSATTGAYLNSVMDDMALV
                     NLVPGGQPTAIISGVDFHGSTLYGINRTEGEIFRVDLSNPAAPGQPELVAVIGSAGFG
                     FIETRDLEVDPRTGLLHITGYHWSKPILGGNYTSAAMLRVNPAGAPNAAVEIFEAPMP
                     SPPGPNNEIWSGPSALAFARPFASLGETVSIGSLVWNDSNANGLRDPGELGIPGVRVE
                     LWHDADGNESNGAEVRIGWTFTDARGHYYFSGQKPGRYQVRVSLINFGDGMPLSGSGI
                     SSPVSSTLDDQTDDDDNGSQPGGARTEVFSPLITLTPGQEPLGNAVTGVETGSGGDLD
                     DFTTDANGDMTVDFGFVETGSMGLGNLVFLDANDNGRHDTGEGIDGALVELYFWGMTP
                     GLDPPIRSTVTANGGRYLFAELWQGQYFVHLPAAQFLSTGVLRGLFSLPGVQAGDDNL
                     GEDGLDDTDPPTQGISTGRVILTKDSTPTNATSETGFDHTSDDDDDANTDLTIDFGLY
                     NAVAAGNMVFMDANSNGRYDDGEGLDGVTLELYQFTQTPGEDTPVVTTVSANGGRYLF
                     DKLRPGNYRVHIPASMFATGAPLFQRVSIPEGLLGDDDAGEDGLNEGDPALIGVSSGL
                     IALFPGSCPADESGETGVDHATDNENDAAVDLTVDFGFQTPVGVGNLVFIDANGNGRA
                     DPGEGVDGVKVELYAGDQNPGVDQSLFSQLTADGGFYFFDFLASGDYIVHIPASEFAP
                     GRALHNTLSMSGAQTDNSDDNLGEGGLDNASPALNGISSRVISLAVNAEPVNEPGLGG
                     NMDAFDDNNFNLTVDLGFLPVNANAVGVGNLVFRDLNGNGRHDPGEGVDGVTVRLFNG
                     MADPLTASPLATTITANEGIYLFSNLAAGDYIVHIPPSEFVSGKPLHGWVSVPGQGGD
                     NGLDDNVDENGSDGDPLTGGVSSVVIALAPGAEPVNAFGETGRDAFMDDASDANVDLT
                     VDLGFHKPVAVGNLVFIDANKNGVADAGEGAAGVEVNLYAEGMDPMFDPPVGSATTDA
                     NGFYLISDLASGSYFLHVPYTQFLFGQPLHGLVSVAGAAAGDDNAGEKGLDDARPDLN
                     GIRTAVFNLVPGQCPVSEPGLGGDSDAGSDESTDLTRDFGFVQPVQIGNLVFRDDNGD
                     GIFDPDFEYGVEGVTVQLWSNSPAVKLAETITWGGLYSFTVAPGSYHLRVPASMFASG
                     GPLEGLTPSAATPNDGGQFFDDDAGQDAYINGSNLFTSGARTAAFTIAHGQAPGEAEG
                     ETGFLYWEDDHMENDSDLTIDLGFMSVDASAGAAPPDEPSGMMNQGGISTDSTFTAWQ
                     AEHEGIGAPGDDPDGDGFPNLLEYALATDPNSGIQPARFTLSVDAATARVDAVLTRPS
                     GGTRDLRYFLEGRTVLDSEWSALPASPVITQNNNGTETARFQDVAAATGPGYLRVKVA
                     LDADLDGTPEATALTPAQVWLRRDFAAQQTFSMPLQRPDLFRGMVYDASNVTLVINAV
                     GLTTNLPENTPLYLEVLTGAHAGHRFEVDESATTDSAIVLDPAAPGSTLGTVPALGGA
                     RIAVRPHWTLGALFPAERFTPGTSSADADRLLLHDAAANEFRVVWLGADGWQGEGDAT
                     RVIQPGEGLLVHARHAPVSLTFTGQLREGPFIHRLDGLTRLIGSGQPAARSPADLGLT
                     TAAGFPAATTSDQAARLRLWLGDTSPGDTGYENLYLHDTGSGPRWILENDPAQADQTL
                     QPLLGPARALFLVPQAPVSILKE"
     gene            complement(46960..48438)
                     /locus_tag="PLP58_00735"
     CDS             complement(46960..48438)
                     /locus_tag="PLP58_00735"
                     /inference="COORDINATES: protein motif:HMM:NF013714.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HRJ07076.1"
                     /translation="MKMLRRLLTSLLLLVIVLSGILAGHLALVWWSDPAAAPPAPEPG
                     KEDVSRFSLANPARVVRVARTLPEAEAQLAGLVRQARAEGGRIAIAGARHSMGGHTMA
                     DGAVAVDMTPLNFVDEPRQEGGAHFVTVGAGALWRDVLPRLQAAGLAVQIMQSNNDFT
                     VGGSLSVNCHGWQHGLPPIASSVVSLRVVTADGGVAACDAHTNGELFRHVLGGYGLFG
                     IITQATLRVVKDAHYKTKTVAVTPESYWKTFQCETLDDSAGMAYGRINVAPLGFLESG
                     TVNVLRLSGESPPKTRPAGFMPLLKRLVFRGSVGSGFGKQVREWGERLGGELDGGWRS
                     VIQGEPASDFGSRRTDSTEILQEYFVPVRRLGEFITRIRPLFEKHRPVDLLNITVRDV
                     KPDETAALPYAREHVFGLVMLLHMELGPESDERMRAFSQDMIEEALACGGTYYLTYRP
                     HATREQFARAYPMAADFFETKRRLDPGNIFRNQFLEKYGPGK"
     gene            complement(48530..49561)
                     /gene="fbaA"
                     /locus_tag="PLP58_00740"
     CDS             complement(48530..49561)
                     /gene="fbaA"
                     /locus_tag="PLP58_00740"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012419773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004332 - fructose-bisphosphate aldolase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase"
                     /protein_id="HRJ07077.1"
                     /translation="MPVATPAQYRAMLDAAQKGGYAYPAINVTSLPTINGALKAFAES
                     KSDGIIQVSTGGGEFASGTAVKDMALGAIVLAEATHILAERYSVLVALHTDHCHPKNV
                     EKFLKPLLAATKARREADKGNLFNSHMFDGSELPLEENMRVSKELLQQCAELDIILEI
                     EAGVVGGEEDGHDTSGVANEKLYTTPEDMLAVYETLNGIGRFMFAATFGNVHGSYKPG
                     AVKLKPAILRDGQKAVMDKHGAAAEMDLVFHGGSGTPLEEIRETLGYGVIKMNIDTDT
                     QYAFTRPIVDHMMKNYAGVLKIDGEVGDKKTYDPRSYLKKGEQGLCERMKEACDDLLS
                     TGRTIFGTV"
     gene            49682..49756
                     /locus_tag="PLP58_00745"
     tRNA            49682..49756
                     /locus_tag="PLP58_00745"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:49714..49716,aa:Val,seq:tac)
     gene            complement(49773..50750)
                     /locus_tag="PLP58_00750"
     CDS             complement(49773..50750)
                     /locus_tag="PLP58_00750"
                     /inference="COORDINATES: protein motif:HMM:NF013694.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HRJ07078.1"
                     /translation="MIRLITQQGQLIDWNDEKVRPFPDNLVFLDLMNPSRAEELESES
                     WLEYQLPTREEMQEIEESSRLYMEKGALYMTAWIPVGLDTPDPDTTAISFVIAPDCLT
                     TVRYADPMAFRLLADTVRRQCSLPTTSDAVFLTLCELIVARIADALQTVESDLKKVGR
                     EIFSLRPHEANAAIEKDLGDVVRTLGRRSALVANLRESLVSVNRMLQFFLNNAATWLR
                     GDLAAQFRSVMRDVKSLDDYTNQQQQEMTFLLESTLGLINIQQNQIIKIFTIASVLFL
                     PPTLIASIYGMNFEHMPETKWPWAYPAVIVLLILSALGPIWFFKKKKLL"
     gene            50874..51986
                     /locus_tag="PLP58_00755"
     CDS             50874..51986
                     /locus_tag="PLP58_00755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009958455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-TA family PLP-dependent enzyme"
                     /protein_id="HRJ07079.1"
                     /translation="MSLHAWFHVDNEAEIASPALLLYRPRIEHNLRLMLETAGGPARL
                     RPHVKTHKLLPLTRRQVELGITKFKTSTIAETEMCADAGAADVMLAMTCVGPNAHRLA
                     RLAEKHPGVQFSAIADDEESIRTLASAAQQHRVTLGVFLELDCGMGRTGIVPGEAAAF
                     LVHVIKDTPGLIFRGLHAYDGHIHDAELAVRRERAEAAFAPVLAFRQRLEGEGAHVAE
                     LVAGGSPTFGYHAGHADRICSPGTTVLWDFGYGERHPDLAFLPAAVLLARVISKPGKD
                     RLTLDLGHKSVAPENPHPRVKFEELPEAEVLMQSEEHLVLHTERAHEFLIGQALHGIP
                     RHVCPTVSMHGEAVVIDRGRAVETWPVTARNRRITV"
     gene            52079..52534
                     /locus_tag="PLP58_00760"
     CDS             52079..52534
                     /locus_tag="PLP58_00760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009965943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MOSC domain-containing protein"
                     /protein_id="HRJ07080.1"
                     /translation="MRLLHLYISPEHNYYGHHGQPAGTAPMLEVDEVECVAGKGLRGD
                     RFFGYKEDYKGQVTFFEHEVYERLCAQFGIHDLPPSVFRRNIITTGADLNELIGGEFE
                     VQGVRFIGTQESAPCHWMNQAFAEGAEDALKGHGGLRARILSDGILRRE"
     gene            52571..>53044
                     /locus_tag="PLP58_00765"
     CDS             52571..>53044
                     /locus_tag="PLP58_00765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ07081.1"
                     /translation="MPRRSFTFPIVIILLPAALAAWLLATDHVARSLGVVLLSLLVVL
                     LLCLWWALGARGRRLKRLALVLLAGAALWGAGRLLLRYEGSADGTAMPRFAWRWTVSA
                     SAAPAPQAKAAGLDLAEIPAGLADMRAFMGASGDGIFPAPAWKTDWAAHPPREAWR"
BASE COUNT         9231 a        17345 c        16992 g         9476 t
ORIGIN      
        1 gccggcgctg gcggtggccc ggccctcgct gccgagttat ctggaccgcc agcagctcgt
       61 gacccgcggc gcgggcgggg aggtgcgcgt gctggacaac catctgtggt ccgagcctct
      121 ggacagcggc atcgcgcgcg tgctggcggc gaatctcagc cgcctcaccg gctccaccgc
      181 catcctgccg gtgggcaatt tcatcacgct ggactacagc gcgctggtgg agatgcgggt
      241 ggagcgtttt gaccccgatc cctccgggaa cctggtgctg gagtgcgcct ggaaaaagca
      301 gccggtctcc ggcgcggaca cgccctttaa aagcttccgc gccgaggtgc ccgttgaccc
      361 ttcgaaagcg ccgatgacgg gccgcatcgc cgccatgaac gaggctctgg cgcggctggc
      421 gcgggagatg gcgcgcgggc tttgagggcg ggatgagggc ttgcagtccc ggcgcggtcc
      481 tttagtgtcg ccacttttcc cgcccgccac ccgacatgcg tttccgtaat ctcacccgcc
      541 gcctcgacat cggggcgaac agctaccttt tcgaatcagg caaacacaga gtcctggtgg
      601 acgcgggcat ggatccgaag gagacgggcc tcaaagcgct gccggacatg gggccgctgc
      661 cgcaccgcgc ggcgaacgcc attttagtca gccacgcgca ccacgaccac atcggcgcgc
      721 tgccggtggc gcagcgggcg cacccggaca cggatgtctt catgacggag cctacgggcg
      781 aggtgggcac cgccatgctg cacaactcgg tgaacgtgat gaccagccag cgcgaggaga
      841 aggccatcac ggagtacccg ctcttcaccc accgggagct ggatgaaaac cgcgcgcgtt
      901 atgtttatcg ggatctggac cgcccgttcg agctgccgga caccgacatc accgccagct
      961 tccacgacgc ggggcacatc atcggctcgg cgggcatcac gatccggcag aacggcggca
     1021 cgcttttcta caccggcgat gtgaacttcg agccgcagac catcgcgcgc gcggcggatt
     1081 ttcccgtgga tggcatagac gtgctcgtca ttgagacgac gcgcggcaat taccagcggc
     1141 cggcggatta ctcgcgcaag gccgagaagg agcgcctggc ggcgctgatc cgcgagacgc
     1201 acgaggcgaa cggctcggtg ctcatcccgg tcttcgcgct gggcaagacg caggaggtga
     1261 tgctgatgat gcacgagctg cacgaggagg gtctcatccc ggaaatgccg ctgcacatcg
     1321 gcgggctggg cacgaagatc accgtgctct acgaccacta caccgaccgc acccggcgga
     1381 actacgcggg cttccgcctg cttgaggatg tggagatgct ggtgccgccg cgccggaaaa
     1441 aaggcccggc gcgcaggcac atcgaatacg agccgcgcgc gatctacgcg ctctccagcg
     1501 gcatgatgac ggagggcacc acgtcaaacc agttcgcggc tcgcttcatt gacaatgcgc
     1561 gcaacgcggt ggctttcgtc ggctacacgg atgtggagac gccgggctac cgcctgcgca
     1621 atgcgaggcc gggcgagaaa atccgcctcg cgcccgacca gcatgaggtg ccggtaaacg
     1681 cgcgtgtcgc ctcctttgac ctcagcgccc acgcctcgcg cgaggccatc gccgaatacg
     1741 tcgagaaggt gcgcccgaaa aaggtgctca tggtgcatgg cgagccgccc gcgcaggact
     1801 ggttcgccgc gcgcttcgcg gagacgctgg gacgcgagac ggaggtcatc cgccccgagc
     1861 cgcacatggc gatagacctt tggtgaggat ggggcggcga gaaaaccgcc gcctactgga
     1921 tgatctgcac ctcgtcaccc acgcgagtga tttcgaagaa tttcttcgcc ttgtcataag
     1981 gcaggcggat gcagccgtgg gaggcggggc gccctgtgac atagccgctg tgcaggccga
     2041 tggcgccgca gttcaggcgc atgaaaaagg gcatggacac atgatataga gtggatgtcc
     2101 aggagcggtg cttgtcggtg atgacgtagc gccccgccgg cgtggcgtaa ccggcgcggc
     2161 ctgtggagac cgggcaggag tcaatcacct ggccctcttt cgtgacccac gcgcgctgct
     2221 tgccgaggtc cacggtgaca aggatgcgcg gctcggactc gggctggcgc ccgaggatga
     2281 tgcgcatgag gaaaagataa ccctgggtgg cggcgaagtt gatcggataa cgcttgtagc
     2341 gcgtggtgca gagggaggct ttggcgccgg ccttgagaag ctcggcggcg ccctccacgt
     2401 ctccgcgcga ggcgcaggcg atgagcgggg tgaggccgcg gtcaacctcc aaataatggc
     2461 gcagatcctt gatggtgacg cgctccagca cggatttggc caccggatgg ttgaagcgcg
     2521 tgttcggatc ggccccggcg gcgatgagcg cgtgcaggat gtcactgccc cggcgcaggg
     2581 aggcgagcgt gatcggtggc tgcttttcga aattcatcag cctggggtcc gcgccaccgt
     2641 aaagcaaagc gcgcaccgct ccagtggcgc cgaggcggat ggcggcgcac agcggtgtgt
     2701 cgccgccggg cgtggcctca tctggattga ctcctccggc gacgagccgg ctgatccact
     2761 cctcgtcgtt gtacatgacc gcgagctgga tcaccggcag cattccaaga tcaggaagat
     2821 gcgtgcggaa gtattcctcc acggaatccc gcagcggtgt ccactgccag ggcttcggct
     2881 tgggcgcggg cgcgggtttg ggcggtgtga tggcgacttc cacggctgcg ggcggctgct
     2941 ccacgggcgg cttcggtggc ggcgaagatg gagtggcgac aggagcggtg ggggtgtttg
     3001 gcggtagggt cagtgcggca gggtcggatg acggcacttg cctggtcagc gacagcgagg
     3061 cgaggacttg aagcgcggga gcgggcatgc tcatgccgcc ggcaaatgcg agaatgccac
     3121 cgccgaccgc agccccaaca atgagaggca gtagccaggc aaaaagtgtc tgaatcacgc
     3181 tggattttcc agtcatcggg ccgcaatggt tagcggcgcg tgtgccagtg tcgagcactg
     3241 cctgaaaaat ttactacgcg acatttcaaa tcactgccaa ccattagaat caccatgaaa
     3301 aattccaaaa gcaccgacat caaggtcacc tcagccagcg tcttttttct accagtgacg
     3361 atgcgggtgc cgctgaaatt tggaccagaa acagtgacca acacggtgtg cctgcgggtg
     3421 aaggtgggtg tcgaagaccg gcaaggacgc catgcggaag gctggggtga gacgcctctg
     3481 agtgtgtcct gggtgtggcc gagcacgttg agcgtggcgg agagggcggc gcgcatggac
     3541 agcttttcca tcctgctcgc gcagcggctt gtggcatcgg gcctgagcgg gcatccgatg
     3601 gagatcggtc atgacttcat tcacggtccg ctggcgcgga cgctggccga ggccaatgcg
     3661 gaggcgggcg gaccggagat gccttattta ggatcgctgg tggcgttcag cgcctttgac
     3721 attgccgttc acgatgcgtt tgggaatctg ctcggagtgg atgtctattc gacctacggg
     3781 ccggatttca tgagccgcga cctttccgcg tatctggagc cggaggcggg cagcgggctg
     3841 agcttcgtgg ggctttatcc gcaagatttt ctggcgcgcg aggcgccgtc gaaactgccg
     3901 gtgtggcacc ttgtcggcgg cgtggacgct ctggaggagg cggacctcac aggcggcgag
     3961 ccgcaggacg ggcacccgct gttgctggcg gactggattc gccaggacgg cctgaaatgc
     4021 ctgaaggtga agctgcgcgg caatgacgcg gcctgggact atgagcgcat ggtgcgcgcg
     4081 ggccgcatcg gcctgccgct gggggtgcgc tggctgagct cggacttcaa ctgcacggtg
     4141 cgcgaccctg cttatgtgaa cgagatcaac gaccgtctgc tgcgcgacga gccggagatt
     4201 tacgcccgca cgctctatgt ggagcagccc ttcccgcatg acctggaggc caaccagatt
     4261 gacgtgcgca gcgtctcggc gcgcaaaccg ctcttcctgg atgagagcgc gcatgactgg
     4321 gagtttgtgc ggctgggccg ccgcctgggt tggtcgggcg tggctttgaa gacctgcaaa
     4381 acgcagacgg gcgcgctact gagcctgtgc tgggccaggg cgcacggcat gccgctgatg
     4441 gtgcaggatc tgacgaaccc gatgctggcc atcatcccgc atgtgcgcct ggccgcgcac
     4501 gcgggcacga tccagggcgt ggagtgcaat gcgatgcagt tttaccccga ggcgtcagtc
     4561 attgaggagc gggtgcatcc ggggctttac cgccgccgtg aaggcatggt ggatttcagc
     4621 acgctgcggg gaccgggttt tggctgccgg gtggaggaaa tcgcgcgagt tctgccggaa
     4681 cccgccgccg tggccggctg atcatgcggg gccgctttcg atgacggccc aggcgttgtg
     4741 attgtggatg gactcgaagt tttccgcctc gacgcggaac caccgaacgc gcgggtgcgc
     4801 gcgggctttg agcgccacgt tgcgcacgag atcctcgacg aagacggggt tttcataggc
     4861 ggcttcagtg acgtgttttt cgtccgggcg cttgagcagg ctgtaaagtc cgctgctggc
     4921 ggcggactcg accatctcaa tcatctcctc gatccacacg ggctggtgga aacgcacgga
     4981 gaaggtgaca aggccgcgct gattgtgcgc gccgtggcgg ctgatggcct tcgagcacgg
     5041 gcagagcgtg gtgacgggcg cctcgacagt gacgacgaag tccagctccg cgccgtcggc
     5101 attgacctcg aagatgatgc cgtagtcgag catcccctcg gcctgggtga cgggagcttt
     5161 tttcacccgg aaccacggga agcggatttc caaatgggcc ttgtcagcag cgaggcgctt
     5221 ttgcagttcc tgcgggatgg cggcgacatc ggcgacagtg agctcccgcc cgtgcgcgtg
     5281 cagcacctcg acgaagcggc tcatgtgggt gcccttgaaa tgatgcggca ggtccacggc
     5341 catggagacc gtgccgacgg tgtgctgcgg attggcgtcg cggtcgcgga cgcagagggg
     5401 gaagcgcagg tttttgatgc cgacgcggtc aatgggcagg cggcggtcgt cgcgttcgtt
     5461 ctgggtgtct ttgagggcgg gcattgctgg gaaaaaaatt cccactgccg cggcgagcaa
     5521 cagtgggagc tttggaaaaa atcgtgaccg gcggcgcgcg ccgtgtcagg ggaggagatt
     5581 gacagagcgg gcgcgcttca cctcgcgggt gaccttgcgg tgcagccagg cgggcaggcc
     5641 ggtgacgcgg cgggggatga gtttgccggt ctcggtgagg aaacgtttga gcagctccgg
     5701 gttggtgtaa gtgagcttgt ccaccgggat gtccatgcgg cggcggggca tgcggcggtt
     5761 gttccggcgc agggagatga ggcgttcttc ggttttgggt tgcatgagaa tcgtggatca
     5821 gcggatttcg cggtgcaggg tgcggcgctt gaggaagtgg ttgaacttca ccttctcaag
     5881 ccggcccggt gtgcgcacgc tttttttgtt gcgcgtggtg acgtagcgcg aggtgggctt
     5941 gccttcggcc ttggcctccg tgcattcgag gatgatgatt tcgcgtggca taaaaaatgg
     6001 ggcgggaggc taggttattc ctttccgaag tcaagcgact cgttgttctc gttggatttc
     6061 gatttggcgg gcgtgacggc gtcgtcctcg tcggagtcct cggagtcgtc ggcggcctcg
     6121 tcgaggccga agagcgagcg gaccgggcgc gtccagcggc cgccgcgctg gtcgcgctcg
     6181 atgcgcgcct ggcagttgac ggtaaagcgg gtgaagggca gggcttcgag gcgctcctcg
     6241 ggaatggtct cgccggacat ctcgcagatg ccgtaggtgc cgcgcgcgat gcggttgagc
     6301 gcttcgttga tctcgtaaat ggcgtcctgc tccttggcca gaaggctgag ggcgaagtcg
     6361 cggtcatagg cgtcactgcc ggcatcggcc tgatgcatgc cgaacgcgga ggagtccccg
     6421 ctctcatggt tgcgcaggct ttcagcggcg acaccttcgg cggcgttcat gtaagcgtcg
     6481 cgcagctcga cgagccgctg tttctgcttc ttgaggaacg cggcgggcag cacgggtttc
     6541 ttcggcggct cgaccagggt gatcccgtcg tcaatggagg atgtgtcgcg cttgacggtg
     6601 atgatcttgg cgccgttggt caggcctttg ggctttttgg cggtggtctc cttgttttga
     6661 acgggcttgg ccgcggcggg tttcgcctct ttggcgggcg gagttttttt gaccggggcc
     6721 ttggctgcgg gaggcgcttt tttcaccggc gcggcgggct ttttggcggc cggggttttt
     6781 gaaacggccg gctttttgga tttggccggg gctgcggtct tggcggcttt tggggccggt
     6841 tttttggctg cgggtttgga gggggatttc ttcactgggg gggatttttt tggcgccggt
     6901 ctggctgcgg cttttttcgc cggcttggcc ggggctctcg cggagacttt cttcacgggc
     6961 ttggcgggcg cctttttggc gggtttcgcg gaggctttga ccgccggttt tttggcggtc
     7021 ctggcggggg ctttgggcgc gggttttttc ttcgcaggca tggcgcgtga ggttggcgtg
     7081 gtttcgctgg agactgccgc ctgtcacctg acgggcggaa aaagagcgcg gagtttggta
     7141 gggtttgcca gggatggcaa ccggaaatcc gcctttctca cacgcgccga gcctgccttg
     7201 caaagagctg cccgcggcac ggatcagcgc ccgtcggcgg agtatttgcc cgcgccggtg
     7261 aagaccagcg ccaggtagcc ggtgagatag acgaaggcga gctcgccggg cttatctccg
     7321 gcaaaggctc cgccgtgcgc gatgaagaat gcgaccgcca tggtgacggc gagattcaac
     7381 gcggcaaagc gtgtgaacag cccgacgatg agcagcacgg agcacaccac ctcggcgaac
     7441 acggccagcc caaggctgat gtgcgagggc aggccgaaga gggagagaaa gccgggggcg
     7501 atctcgctga agttctcgaa cttcttccat ccgtgcagca gcagcatggt gaagccgagg
     7561 gagacgcgca gcacgagcag tccgagatcg gaacagccgc tgagaaagga gaggcggagg
     7621 atgttgagca tgacggggga tgatgtggcg ggccggggcc gggtggcaag acaaattccc
     7681 gcctcacggg tgatccgcgc accaagccag cacctgggcc cgctgggccg gcagccgttt
     7741 gtgcagatga tcctccaaaa tgcgcgccgc ttcggagccg ggcgcgcctt tttccccaat
     7801 caggccgaga tcctcggcga cgcgcagttc gaagcgctcc agcatggccc ggctcggatc
     7861 atggctcccc aggaaatcga gcgccctgtc aagcagctcg tgcaaggcgg gcaggggcga
     7921 gtgcggctcc acggcgagcg tcagcaggcg cgtgaaatag gtggcggcga gcacgcgccc
     7981 gtagctggcg cgcaggccca gccgcgggtt caggcaatgc gcctccgcca gggtgtgcag
     8041 atcacttttt ttggccggga caaaacgaat ctcggcggag acgaagagat caagcacgcc
     8101 ctgaaaaggc gacttcggtc tcagcgcgcc tttggcgatg gtgcggaaaa gtccgaggcc
     8161 gggcgagcac cagtggatga tgaggctggt ctcggaccag cgcgtgcgcc cgatgaggat
     8221 ggcctcggtt ttctccatca cggggcggcc tcattgctcc agcacatagt cgccctgggt
     8281 cggctcgacg agctgggatt tgatgagatc gaacgagccg ccctggaagt aggcgtgggc
     8341 gtagaggcgc tgtttgcgct gctccaggtt ctcgcgcacg gtgcggacct ggcggaaatc
     8401 cacgttgtcc tgaaagtcgg agaaccagta caagccgtcg gcgtcacgca cctggtcgct
     8461 gagcagggcc agccaggcgt agctgatgcc cacattgtag aggaagtagg tgcgggggcg
     8521 ttttttgagc aggttgtaga gatcctggct cggcatcggc tctccggctg gagggcggta
     8581 gctgcgcgcc tcggccaggg tggcgccgcc tgtctgccag agcaggcgcc agaatttgtc
     8641 aaagtccggc gtgctggtgg cgatgacgtt tttgccgtcc aggccgcgcg gcggcggcgg
     8701 ctccagaccg cagccgatgt agagcacgac gacgctgcct ttggcgacct tgtccacttc
     8761 ggtgatgacg cgcggcaggt gcggggccat ggagccggag acatcgagca cggcggcgag
     8821 cttttttgcc ttcacctgca tgccaaagag ggagaagggc tggaaggtca cgccactggc
     8881 gcctccgatg cccatgcctt tgccaaagcc gccgcctgat ccggcgagac ccatgccgcc
     8941 gctgagcttg ccgccgccga gcaggctgga ggcttcggga atgtcgagca ggtcgggagc
     9001 gtcatccggc aggacgaggc tgttgatgga gttggccacc gccagcttgc gcatgggcac
     9061 gggttttttc agccagggtt ttttcttctg ctgcacgcgg tgctgaagcg cggctgccgc
     9121 ctcggccccc tgcttggtgc cgccgccgga gaggaagtcc acctgctttt cctgcacctg
     9181 gctgacgacg atccacgcgg cgctgagcag cagcagcgca tgcacggcga ggctggcggc
     9241 cagcgcgcgg ccgccccatt gatcccagta ttttttccac atccgcgctg tgcgcgcggt
     9301 gaggccctta tcctcggaag cgggcgtctg ggccgggtct tgggactgtg tgtcagggtg
     9361 atcctgggtt gaggacttgt cagatgggtt ctccggtgtt ttggcttttt ccgtggactg
     9421 gtctgtctcg gaggtggtct ttggcgcggc ggatttgtcc ttgctttcac tgccggtgct
     9481 gctggaacgc gccctggcgg gaactttggc gaggggtttt gacgggcgcg gcgctgtttc
     9541 tttagcgatc gggcggggtg gttccgattt tcgcggcggc tccgccggag gtgcgctgat
     9601 ttcagagggg cgttctccag catcgcggga ctccgccgga gggcgggcag gattttctgg
     9661 cggggaggag aaagattcgg cgggagcctt ggacggcaat tccgaccggc gcgctatgag
     9721 gctgcgcctt ggcgcgggtt tggccggagg cggcggtggt gacgcagcgg attcggaact
     9781 ctccacagga ggggttttgc gcgcgatgac actgcgcctc ggaccagcgg cgggtactga
     9841 atcctgcgcg gaaggcggtg gctgggtcag aggctgggcg ggaacctggg catcctgcat
     9901 ttccagcagt tcctgccatt cgggtgaaag agggcctgac tgagccggaa ctggcggcgg
     9961 cgatgtggag gcgggaccga tgcgggagcg ggttttgccc tgctccgatt tccagagcct
    10021 ttccccggcg gtcatgccgc ccgcgctgtc cgcagtgggc aggcggctga aatcgtcttc
    10081 tggattttcc ggcggcatgg agcgtaaagc tgcctcaccc gcgcgcagga atcgagaaga
    10141 aagtgattcc aacgcacgtc ttttccccga tgtgggaagg agaatgctca gcctgcggcc
    10201 attgaacgga cagcaaattc acaaagagga cagtgtgctg ggaggagaaa gccgcttccc
    10261 gcagcctgga acaaaaacag ccacttatga ggaatgatca aaacagcctt caaaaccgaa
    10321 tggtggcagg gtgtggaagt ggtgccggag gacgccaagg aacccaaggc ccacaagcag
    10381 aggaaaaaga aaatggccaa gtctggcggc cttaccaaga tgtcgaactc ggttcttggc
    10441 ccagatctgg acactgattc tcgctactcc caaatggaag agaatttcag cctctacttt
    10501 ggactagccg tccagcttta tgaggccacg ctcgtctcgg atgacacgcc gtttgaccgt
    10561 tttgcggacg gcaaaaaaga cgcgctgaac aaggagcaga aagaagggct ggatctcttt
    10621 tttggcaaag caaagtgcgc caactgccac gggggtcccg aactgaccaa agccacggtg
    10681 gggcacatca aaaaggagcg cctggagcgc atggccatgg gagatggcgg catggcagtt
    10741 tatgacaatg ggttctacaa catcggagtg cgtccgagtt ttgaggatct gggcctggga
    10801 gttgacgatc catttggata tccgctgtcc gagtccagag tcgctcggat ttacggcaaa
    10861 gacggtttca aggccctcat tggtcgtgac cccaaccttt cagtcggtca gcaggagcgg
    10921 gtagcggcgg acggcgcctt caaaaccccg ggcttgcgca acatcgaact caccgcgccc
    10981 tatttccaca atggtggcac caggaccctg ctggaggtgg tggaatttta caatcgcggc
    11041 ggtgatttcc acgacaagaa cattcaagac ctcgatccgg atgtggagcg tctgcatctc
    11101 agccagaaag aaaaggaggc attggttgct ttcctccact cattgacaga cgaacgagtc
    11161 cgctatgacc gcgccccatt tgatcatccc cagttcttca ttccaaacgg tcacatcgga
    11221 aatgagaaca ccgtctcgaa tgaaggcaca ggccgggcca aggacatcct catcgaagtc
    11281 cccgccgtgg gagcgaaggg cggagcagcc cggcccaact ttctggaatg aggaaattca
    11341 attcgagtat aatatccata aatactattt gatacgataa ttaatgggtg ttaataaatg
    11401 catgagcatt tatgcacccg ccactttcat cgccactctc agcatcctct gcgaatcacg
    11461 gttcgcggag gattgcgttt cgggaatcat gcttgttgtt ttgcgtttcg aactggcggc
    11521 gtcagatgct ggtgctctgc gcctgccttg gcctagctgt gggtagagcc ggcgctgcgg
    11581 gcaccatttt ttggtccagc cagtttcagg atttgctctt cgacagccag ggccaggagc
    11641 ttgatcatag ctttgccttc gagatcggct ctttcggcag tttcattccc acctatgcca
    11701 acatgtccga ctgggaggca aattggaagg tgtttgaccg cgccgtggac gggaatggct
    11761 ggtttgctga cgaccagttt tttttcggcg cggcggatca caatgatctt ggcggttcgg
    11821 actcgcccgc cgccgatcca ctggatgtct tcctggctgg tgagaaagcc tatttttggg
    11881 tgtttaacag caaggccctg gcagctggca gtgaatgggc gctggtgacg gacagcgatt
    11941 ttttctccaa caccggagac tcctggatct ttgaggactc ctcggacccg ctttattttg
    12001 gctccgactg gatgttggag gatgcggatg aaatcatcgt tggtggtttg ctcaatgtgc
    12061 agggtgatgg tgagttcagc acgctgcccg gggcgttcac gctgcaaaca gcggcggttc
    12121 cagagccggg ttccgcattg ttgttctggc tgagctgctc gaccctgctg ctacgcaggg
    12181 tgtcaaaacg attgggcatt ttggcataag gagatggact ggagtttaag aacttgaggt
    12241 ccatgcgtca aaaaataaag agatcttaaa ttctgagata tgctgcatat ttttgcattc
    12301 ttgttgattt cgttgttttt atggtaatta tagtcgaatg ccacgctgat gcctcttttc
    12361 gcccgtctag ttcctctcaa tgcataccgc caggatcgtg aaaaactcgc gagcgacggc
    12421 tggggagcct tgcttcgaaa tctagcggtg tttgtcataa tgattcacgt tctgtgcgcg
    12481 gtgaggctcg aggcgcacaa cttgaatcac cgcatcaatt atgtggcctt cgatcatgag
    12541 acgatggaaa tgatcaaggc gcgcgcgaag gcgggtcagc aactgctgag agcgaatgac
    12601 atcgtgggtc tggtgatgaa ggcgacaccg gatgtcggca cacccacagg cgcgggcggc
    12661 tattccacct tctttatccc cgtcggcggc caggtggtgg gcgctcacta tgggttcctc
    12721 aataaaaacg gcgagtttgt catcaccccg atgaagggcc agtccatcct ggctcttgga
    12781 gacgggtcca gcggctcgaa aagcagtccg cagttggtgg ggctgcagtt ggggccgaac
    12841 atcacggggc agacctcgct tgtggtggat aattcgggcg cgcaccgcgg caccatggcc
    12901 ggattatact ctgacaccgg aattttctac tccacggacc cggccaccgt ctggatgagc
    12961 tgggcgacgg gcggcggcat ggacggaaac ccagccacat tggataattt gatggtcaac
    13021 aactccggtg acgtggttac gccgacgacg cgctgggatg ccgagcagtt gctggcctgg
    13081 ggttcgaaaa atcctcccgc attgattgac tcgcaagacc aaaggggaaa cgcgccctgg
    13141 ggcatggcca gcgcggtggc cggtcccgag agcggctacg cctggcattt caacaaatcc
    13201 atctgggatg gaatgtccgc gaatccaaac cggatgaagc tctccgtcag caacatggga
    13261 ccgtggaagc gcatacgcta cccaggcagc atggccgcca aggacgtgcc tggacgaaaa
    13321 ggcagcgatt tgggttatgt gggcgtgaac gccgcagaca tcggccatga cttgaacacc
    13381 ttcaacccgc tgccgccgac gacgagctgg acggacagca cgagtccgaa agccatccgc
    13441 atggcatggg gaaatttgga gctattccgt ccggagtact gccgggtgag gctgaaaata
    13501 ctcaaggggc ccggcgagtc cggctcaccc ttcgactcgg acggctttct tctttcctat
    13561 gcggacacct ttggcggcga cgctggcggc gaatacaaca acaaggacca cctctggcgc
    13621 tactatgagc cgacccgcac ggaccttgcc gggaagccgt tcatcgccaa gcacgcctcc
    13681 aagcccatcg tcgccagtgg ggagaacttt cattatgaca tcaccgtggt gaacttcgga
    13741 ttcaacacgc tgaccaatgt catcgtcgag gatttgctgc caagcggggt cagctatgtg
    13801 agcgcccagc cgccgctcac cagcacctcc tcgccgctga cctggaaaat ccccagcatg
    13861 gcaccgcaga ctgtgaaaac cttcaagctc aacgtgcgcg ccaccggctc cggcatccag
    13921 gtgaacacgg cctccactcg cagtgaccag accccgaaaa cgagcacgct cgaaattatt
    13981 gacatcatca gccaggccct gctctacccg gacaaagccg tgacaccttc gacggcgcag
    14041 cctggggagt tggtgacata cactctggtc atcgaaaaca ccggcacagc agccagcgcc
    14101 acgcccatgg tcatcaccga gatgctgccg gacggcttcg tctatgacag tctcgtgtcc
    14161 gcccgcgtca acaacgcgcc ggtttcgaac ggctccttga gtgtgaacag cgcgaactcc
    14221 gcgcggcctg tcttcaacct gagccaggcc atcaacgcgg atggaaagct ggagatcacc
    14281 ttccgcgcca ggatcagtca aggccagccc gccggcactt acaccaacag ctacatctat
    14341 gcctacagcg gcaaggtcat gtccaccgga gcgctggctc cggtgaccgt gggcggcgcg
    14401 cgcctgggcg ggaaagtttt cagggactgg aacggcgacg gccagatgat cgccggcgag
    14461 gagggcatgc caggtctttc ggttcagctt tggaatgcag cgggcaccac cctgattggc
    14521 agccgcacca cggacagctc gggcggctat gtgttcgccg gcgtggcctc cccggcgact
    14581 tatcaggtgc gcgtggaggg tgttccaacg ggttacacgg ctacttatga tctcgacggc
    14641 gtcagcagcg ccagccgcgc ctccgtgccg ctggcggcgg ggcaggaacg gatggatgtg
    14701 gatttcggct accagcctgg cggcgctgcc ggagtgggcg gacgagtgtt taacgacact
    14761 gcaaaggacg gcaaatatca ggccggtacg gactccggca ttgcctccgt gacagtcaga
    14821 ctgcaccacg accgcaacca ggacggcaaa cttgacgcgg gagacttgct gttgagcaca
    14881 gcgaccagtg gcgcggaggg cgtttacaac ttcaccgggc tggcccccgg cctgtcctac
    14941 cttgtgcagg tggacgcgga ggatgccgac attcccgccg ctcttggcgg tcagcctgtg
    15001 cgcatcacca cgccgggagt gctggcggtg cccgcattga acagccaggt cacaggcaag
    15061 gatttcggct tctggaaaga gcagccctcc tcgctcggcg gcagggttta cctggacaac
    15121 aatggcaatg cagtctttga cgcgggggac acgccggtga cagggatacc cgtgcggatt
    15181 ttccacgacg cggacggcga cggcacggcc cggcagtccg aactcgtggg tgtggcgccc
    15241 agcgatggtg gggggcgcta ccgcttggat gacttgcccg gcggggattt catcgtgagg
    15301 ctaggcacgg aatccatctc catgccgccc ggctaccgcg cgttgagcga agagcatttg
    15361 cttgttctgg agacgggagc gtcaaggctg gacctggact tcatgcttgc ccagcttctt
    15421 tccaagatgg ccaaccgctc ctccgcggtt cagggcgacc agattgtcta taccctgatc
    15481 cccaattacc agggtgacgg tgccttggaa aacttgacca ttcaagacgt gctgcctgcc
    15541 ggcacaacct tcgtcagcgc cggccagggt ggtgcgcttg gcagctttgt ctcacagccc
    15601 ggcagccccg ggcaggcggg gaacccagtt gtgcaagtgg ccaacggaac ttacaccggc
    15661 aacggctcgg acaaccgcaa catcaccagc gcgggcttcc agcccgacct ggtcatcatc
    15721 aagggtgcca gcagcgtggc gcccgccttc cgcacctcga ccatgagcgg ggacgccagc
    15781 aagctggcca cggccaactc gcttgcctcc aacatcattc aatccctgct gtccaacggc
    15841 ttccagctcg gcaccaacgc ggcggtgaac tcgaacggca tgcgctatga ctgggtcgcc
    15901 ttcagggcct cggcgggcgt tctgagcgtg ggttcttaca cgggcaacgg ttcctccgcc
    15961 tcccggctca tcaacaaccc gggattttct ccagaggctg tgtttgtcat gccagccaat
    16021 tcctccgccg tggtgatgaa aacggccacc atgatgcaaa acgaatcctt tctgttcacc
    16081 gggggatcag cggccaccaa cttgatcacg ggttttgaaa gcacgggatt ccgtgtctcg
    16141 accggtagca atgccagctc caccgtttat cattatgtgg cctggaaggc catggacggc
    16201 atcacatcca ccggctcatt tacgggcaat ggcgtggatg accgcagcat cacggaccct
    16261 ggattcaggc ctgagttcgt gctcatgcgc agcgcgtcat cctcggcggc ggtcatgcgc
    16321 ccggcttctt tgactagcga tttgtccctt ttcggcagcg ccacggcgag cgcggcaaac
    16381 aacattcagt ccctgctcga ccagggcttc caggtgggaa cggatgccag ggtcaacgcc
    16441 agcggatcgg agattcactg ggcggcattt cgtgccgccg ccgccacgcc attgacgacc
    16501 acggcattga acgtctccaa gacactgtgc aaaaacagtg acctcgtcac cctcaccatg
    16561 acgctgacga gcaacagcac gctttcgggc atcctgccgg gttcgcccgc ggtcaacacc
    16621 agcggcagcg ccagtgcggt gctggtcaat ggtccaactc cgtccactct gaatctttcc
    16681 gccggcggca gcggcagctt ctcctggacg tatcaagtgg acgcgggcgc gggcagcgga
    16741 gatgtgagct ttacctgcgc ggcgtcctcc tcgggctatg cgtttgcacc ggggacctcg
    16801 ccttccttga acgtctcctc atcgggcacg ggccagagcg tgacatggaa tctgggcagc
    16861 accagcgccg cggcacccgg ctcgctggtt cagaatctca acctctatgc tttccagggc
    16921 gacaacaaga cgggctttta tcgttacgat gccgggacgg gcctctggca ggcgcgagcc
    16981 agcgcgcccg ccagtgtcta ttggggcggc gcgctggcct cggacggcac gcacttgtat
    17041 gcgctgcgcg ggaacgacca gtcaacgctc tggcgctatg atccggccac caatgcctgg
    17101 acgacccgcg cctcggcgcc cgccgcgatc aaggagggcg gcgctctggt ttatctggca
    17161 cctcattttt atgccctgcg cggcaagagc acggcgactt tctaccgcta cacaccctca
    17221 accaacacct ggagcacgat ggcatccctg ccggccgcgg tcaatgcggg cggcgcgctg
    17281 accacggatg gcacatttgt ttacgcggtg cgcggagatg ggaagaagga gttctaccgc
    17341 tacaatcctt caaacaacac ttggacggcc cgcgcctccg tgcctggagg cgtgggcaaa
    17401 ggcggcgcgc tggcctttga tggcacgcac atttatgcgt tgaggggcgg agactaccgc
    17461 gaattttacc gctacgaccg gacggcgaac acctggaccc gcagggcgga cactccctcg
    17521 acggtcaatg acggcagcgc gctcgtcttt gccggctcaa cgatttacgc gttgcgcggg
    17581 gacaacgcca aggacttctg gcgctacaat ccctcgtcaa acacttgggc ggccatgacc
    17641 agcctgccgg tgaatgtgaa catgggcggt gccatcgtgg attatggggc gcagactccc
    17701 acggagattg gcgtgggcat ccaagtctct ccatcactgc tggcgcatgg cggcacggcg
    17761 cgggtgacca tgacgctcag ttccagcgag gggcagatca acaatgtgac tccgcccgcg
    17821 ctgagtgttc agggcaccaa cggcgcctcc gccaccctgc tcagcggtcc ccagcctgcc
    17881 agtgccaccg tgccgtccgg cggcagcgcc agctttgtct ggacttatca aatctccgcc
    17941 ggcacgaacc cagggcaggt tgtttttgcc agcagcgcat ttaattccgg cggcaagccc
    18001 ttccccgccg ccacttccaa cggagtcatc gtcacccgtc ccctgaggct gaccgtgacc
    18061 gtgacggatc ctgcgggcct tgattcagtg gacaacgtgg caaccatcca gacgggcgag
    18121 tttgctggaa gttatgtggt ggccaatctg acaagcggat cgaaggagga tcgtctgctc
    18181 tccgtggatc agggcagggt gacggatctt ggagccacag gcacgactcg tgtgagagcc
    18241 atcacactgg ccgcaaacgg cggtttatat gcggccgccc gcgattccac catcggcact
    18301 ggagcggatc tttttggcct gattactccg ggtggcagcg cttccacgct caggcaatgg
    18361 gctaacacgg tggttggttt cagcagtgaa tggacaaatg acggcagcag ttggcgggcc
    18421 atccaggcgc tgggagagcc ggacaccttc agcgcgggcg acctgcccac cgcgtggtcc
    18481 ccagccacaa acacaagcaa cccggagtat ctgcatctgg gttatgccac ggcggtgtac
    18541 gccaccctga tccgcgtgcg cgagacttat cttggccagt ttgtcacagg tattgagctg
    18601 gtcgagcctg acggcacccg gcactccatc cccatcccgc cggacggcac tgttgtgccc
    18661 ggctacttcg aagtctcgtt tcctgccacc agttatctgg tgagctcggt ggtcatccac
    18721 acagccaagt cagggtatga ggagattgac gccgttgagt tggtggggat gcctgctgga
    18781 agcggttcat tcatcccgct gggcagggcg gccagcccga ccgaaccgct ggagggaccg
    18841 ctgggcaact tcacgacgct gaccatcact gccatggaca ccgatcccca gacggggatg
    18901 atttatgcca ttcaccggcg cgaggacgcg acgatggcca acacacttct tgatgtgctt
    18961 ttcatcctca atcccgagac cggcctgcat gtcgaggacg cttttggtcc gggagtggat
    19021 tatgtcgcgg tggccaccaa cacattgccg gtgccgcttt atgacattga cgacatcacc
    19081 ttccatccgc agaccggcga gctttacgcg atagccaatg actccgcctc cggcgtttcc
    19141 gaccaggaca ggcttgtggt catcaatatt cagacgggcg cggtgacgga cattggccgt
    19201 gtcacgatca aagccacggg cgagggcatt catgatgccg gcggcctgca tttcgacgcg
    19261 cggggcgtcc tgcgcgccag caccggaatc ggcggatctg cggtcacgaa caacaccctg
    19321 tgggtgctgg atcctgaaac ggcggaggcg gtgcgcgagg tcactttcac cgccgcgtat
    19381 ccggccttca ccgactacca ggcgctggcc tccgtctcgg tcatcagagg catccccagc
    19441 acgccgagcc agaccgtgcg cacggctctc agcggcagca tcggcgacct cgtcttcgcg
    19501 gatctgaacg ccaacggcgt ccgtgactcg ggtgagggcg gcctgagcgg agttcgcgtc
    19561 accgtgacga acggcagcat cacgcgctcg acggtcaccg atggtctggg cggctacaga
    19621 gtcctgggcc tcaatgccaa cggaggccag ccctggacgg tgagcgtgga tctcgccact
    19681 ctgcccgagg attgggtggc caccacgcca ccggtgctcc agcgcgtgct gccagccaac
    19741 ggctccgcca tcaatgacgc ggacttcggc ttcatcccgt cggacccgaa tggcggctcc
    19801 atccgcggcc tggtgtggat ggacacgaca caagatgggg cggctggaga aggggaaagc
    19861 ccgctggccg gagtgagcgt aaggctttac cgcgacttga acgacaacgg cgtcgtggat
    19921 tccggcgacc tgctcatgcg cgagcgcgtc actgacgcgg agggcggcta ccgattcagc
    19981 ggtcttgctc ctgcgaagta tctggtggat gtcgtcgagc cctccctccc gcccggcgtg
    20041 gtgctggtct ccgggccgcc gagcttgtca ctgaccacgg ggttgcaccg cgtcaccctg
    20101 gccgcagcgc agcatctggc gggcgtggat ttcggccaca atcacagcgg cgttattgct
    20161 ggcatggtgt tctatgacac gaatcatgac gggatgcgca cgccgaacgg ggctgacggc
    20221 atcgccggaa ataacgatga cgagcctggc atcgccgaca ctggcgtcag tgtttacgag
    20281 gatgagaatg gcaatggtga gctggacgcc accgagcggg tcgtggcggc gacggtgacc
    20341 aacgcgcaag ggcagtatca actgagcggg ctgccgcccg gcaggtatgt ggtaagagtc
    20401 gaggagcagc acatccccgc gcccgccacg agtccgaacg cgggccggtt tgacaccatg
    20461 ctgccgacca cgggcgaggt ggtcgccgtg gaccttgcgg cgggcggtgc tctgcctggc
    20521 gcggacttcg gctttggcga gctggcgctc atcgaggggc atgtgtttca tgatgtgaac
    20581 tccgacgggc tgcgctctcc agacgagccg ggcctgccgg gtgtcatggt caccctttcc
    20641 ggcacgaggg cggcgggcgg cgctttgtct ctcggtaaga ccagcgccca ggatggctcc
    20701 tacgctttcc tggctccgcc gggcgttttc acactggccc atgccgtgaa tcatcccgcg
    20761 attccggcgg ctctcacccg tgtcaccacg ccggactcct atttcatgga gttgcacggt
    20821 ggtcaggaga tcgtggacat ggacttcggg cgtgaccacg ccgggatggc gcacgggcgc
    20881 gtgttcgccg atgtcaacgg caacggtgtg agggacgcca atgagtcggg catcgccaat
    20941 gtcctggtgg aactttacga tgccgcaggc atcacgccgc gcgattccag ggtcactggc
    21001 atggacggtg cgttccgttt tgatggcttg gaaaacgcca cacaccgcct caaagtccgc
    21061 gctgacagcc tgcctccggg ttacagcgtc atgcccacgg ctgatccggc cgctcctttg
    21121 gatggcgagg ctcccttgac catcagcaat ggcggcacgg tggggaatct ggacttcggc
    21181 taccgcatcc ccactcccac attcacgctc agcgggcgtg tctgggatga cggcggccag
    21241 ggcggcggcg tggcgggaga tggcgccatc aacggctccg aaccgggact ggcaggagtc
    21301 ggtgttcagg cgctggtgga ctccacgggt gacggcgtgg tggatgaaac gctgggcacg
    21361 gtgaccaatg gcgcgggcaa ttactcgatt tcgggcatcg ccaaagatgc gcgcgtcacg
    21421 ctgcgcgtgg tctcctcgcc gctgctggcg cagggctttg cgcagacggc ggaccctgac
    21481 gcggtgctcg atcatcagac ggagatcgcc tccctcgcgg cgaacacgct gggcagaaat
    21541 tttggctacc gcaaagtccc ctccagcctg gccgggaggg tcgtttcagg cggcaacggc
    21601 gacggcattg cccagccggg agagacaggg cgtccaggtg tgacggtacg gctcgcacat
    21661 gaggggccgg acggcattcc tggaacgaat gacgatgtga tccaaaccac ggtgaccgat
    21721 gcggcaggcg cctatctatt caccggtctg ccgcctggct attatctggt ctcgcatgtg
    21781 cctgcggacg gctggcagcc ctgggtggac gcggacggcg ggccgccgga tctcatcggg
    21841 gttgagctgg aagccggcca gactctcgac ggccaggact ttgaggcggc cttgcccggc
    21901 attggcgacc tggtctggaa cgacgccaac gacaacgggc tgttcgattc cggagaaagc
    21961 ggcgtgtccg gcgtggaggt gcggctattc catgccggcg cggaccacgc catcggcggc
    22021 acgggggaaa acgccgacac gctggcggcc acgaccacga cggacaacgc cggcctctat
    22081 tcctttaccg gcatggcgcc cgggtattat ttcatctccc tatcgcctgc acagaccctg
    22141 ccgctcgcgg ccaggaatcc ggtgaatgcc gacaaccagg tcaacaacga caacaacagc
    22201 ctccagcctg gcggcgcagg cacggaaatt cgcagcccgg tcattgagat cgctcctgga
    22261 actgagcctg tgagtgacgg agacacggat ccgcatgctg acctgacggt ggactttggc
    22321 ctgcgccctt gcgctgtcat cactctgggg ccggccacgc tgccagctcc tccggtgggt
    22381 gtggcttaca gccagacctt gacggcaggt ggcggccgga cacccagggt cttcgaggtg
    22441 gcagccgggg cgttgcccgc cggcctggtc ctgaatgccg cttcgggtgt catctcaggc
    22501 gtgccagtca gcgtggcggt ggcttccttc accattcgtg tgatggatgc tgatggctgc
    22561 gcggccacac gcgcctacag cctgactccg gtctgcggtg ccatgagttt gaccacggcc
    22621 ggccttccgc ccggaaccgc aggccagtcc tatctgcaaa gcctggaggt ggatggcggc
    22681 aagacgccct tccagttcga tgtcagcagc ggaagcctgc cggcagggct ggctctgaat
    22741 agcgggaccg gtgtcatcag cggcacaccc actgcgggca atggcgcggg gactgcggtc
    22801 accttgcgcg tcacagatgc ctacggctgc caggccgcgc gtgaatacac actgaaaatt
    22861 tgcccggtca ttactgtcaa tccggccacg ctttctgttc ccacggtggg aagcccttac
    22921 tcgcagactc tcactgccaa cggcggcgtg gctccgcatg tgtatgccat cagcagcggc
    22981 gcgttgcctg cgggacttgc cctgaatagc agcaccggtg ttgtcagtgg cacgcccacc
    23041 cagaccacga gccgcacctt cacagtcagc gccacggatg cgaacggctg ccagggcgcg
    23101 cgctcttaca ccctcacgcc tgcctgtgcg ccgattaccc taacgccggc aaatgcggca
    23161 gttgccaatg tcggcacggc ttacagccag gcattcacag ccagcggcgg catcgcaccg
    23221 cacacttggt cggtgtccgg cggcacattg ccagcggggc tggtcttgaa tagttccacg
    23281 ggtgtcatca gcggcactcc cgccgctgcc aacggggcgg gtgtgaccgt cacgctgcgc
    23341 gcaaccgact ccgcgctctg ccagggcgcg ctgaaccttg ttttcaaagt ctgcccgcag
    23401 atttccctcg cacccgccac actcgccagc ggcactcttc aagtgggcat gtcacagacg
    23461 gtgagcgcca gtggcggcgc ggcaccctac acccacagtg tcagcgcagg cacgcttccg
    23521 cccggactgg ctttgaattc agccaccggg cagatcaccg gctcgccaac ccagacgggc
    23581 acttattcat tcacgatcat gagcgccgat gccaatggct gccagggcac gcgcgcccac
    23641 actatggtca tcagcaaccc cacctttgac tttggcgact tttcgctctt cggcagcgcc
    23701 agcagcatcg tctccaacga tttgatgctg ggtgatttcg ttgacgccga agaatttgcg
    23761 gccaccaatc tggcggccac gggcgacgac atcaccaaca tcaatgacga ggacggtgtg
    23821 acgctgcagt ccatccttgt tcgcggtcag gcgggtgtga ccgtcacggt gaagctgaaa
    23881 aataccagcc cggggcctgc ctacctgaac ggctgggtgg acttcaacaa caacggagtg
    23941 ctgtcggaga acgagaggat catctccaat gtcctggtgg aaaatggtgt gacgcaggac
    24001 taccggagct tcttttttga tgtgccgagc aatgccgccc tcggcgtggt gggcgcccgt
    24061 tttcgcctga cttcggccag ttcccccggc agcacgggcg ccgggggttt tggggaggtg
    24121 gaggactata ccaccaccat tgtggccccc tccacaaact tccgggacta cttttatgcg
    24181 atccgcattg cggggaccca gcactacctg gacgagattt cggtcttcaa ccccaactcg
    24241 gccacaccca cggtttccgt ggtcccgggt attttggatc tgaatgtggc gtctccaggg
    24301 ttcaaccccg ctgccacaaa tgccatcatg aacgggctgg cgctagactg gctcaaccgc
    24361 cgcttttact ggctggccac ctcctctgga tccaccggct acaatgccac cttgtacacg
    24421 gctgcttacg acaatgtgag caagacctgg agctaccagg cggtgagcgg ctccgcgctc
    24481 tccaacatcc cattcaacac gggcatgcca acttcagcct ccgccggatc cggcgctttc
    24541 ccccgcgccg cattttacga cggtgaatac tatgccggcg gccagctcaa caataatctg
    24601 gcgatctggc gtctgaactc cagcggtctg ggtctgaaaa cccctgccat cagcgactac
    24661 cccgactttt tccatctgcc tcttaccttc aatggaggag actttgtgat tcgcccgcag
    24721 gacgcgctcc tggtgacttc cacggtgatt agcagctcca acaacctatt gacccaattt
    24781 ttggcggatg gcttcaaccc ctcaggccct gctgcggcgg tcgtggacat caatccccaa
    24841 atcccttatg gaactcatag ttcgattcaa atcgcgggag ttggcggcgt caccagaatg
    24901 tatgctttgg ggtcacaggg cagcacggtt tttcggattg accaatacga caccgcgacg
    24961 cccgccgccg taagagtcgg tcccctgccg aacgcgactt acgcggacct ttccgagggc
    25021 atttccacat cggtgacatc cctcggcgtc aagggcatca tctatcatga caccaatggc
    25081 ctctcggatg gccggctcaa tggaactggc accaacgcgg gaggcgcgct ttttgccttg
    25141 ctggtggacc tttccggcaa tttggtggac gcattccccg tcaagagcga cggcactttc
    25201 atcctggggg gtgcttcctc gaacacgacc tacagggtgg ttctcagcac cacttacggc
    25261 agcctgggag ccaccgcgcc gccagcggcg ctgcccgctg gttggttgaa tgtgggcgag
    25321 ttcctcggct ccggcccggg cagtgatggc actgtggacg ggcgtctgac ggtttcgatc
    25381 ggcaactccg gcctcatcaa cgccaccttc ggcatctccc gcacgctttc cgtcggtgac
    25441 tcggtgtgga atgacgccaa caacaacggc agccgagacc ctggtgaaaa cggcgtcagc
    25501 ggagtcaccg tgcagctttg gtctccaggc tcggacaacg ccatcggtgg cactggctcg
    25561 gctgcggatc agttgctggc cgcctcgacc acgaacgtgt ccggccttta ctcctttacc
    25621 ggtctggcct ctggcagata cttcgtcaca gtgactccac cgccgctgtt ctcactggcc
    25681 agcagcctct cggctggtga cgacggggtg gatggcgaca acaacggttc ccagcccgga
    25741 ggctggggaa ctctcatcca cagcccggtg gtgctcctgg ccgatggcac ggaaccgggg
    25801 aatctcagtt ctggtggcgg cttcttcgac aacaccattg actttggagt tctgccggcc
    25861 ctggattttg gcgaccattc agcgtttggg aacgccagca gcgtggtggt gccggggctg
    25921 cggctgggcg ctttgattga cgccgaaccc gccgccacga cgaatgccac tgccacgggg
    25981 gatgacatca ctggcctgga tgatgaagat ggcgtgacgc tgcctgccag cgtggcgccg
    26041 ggggtctcca gctcgatgac ggtgaatgtc accaacacga gtggcgccgc agcctatctg
    26101 aatgtgtgga ttgactggaa ccagaacggc gtgctgaccg atgcgggcga gcaggtggcc
    26161 ggcaacattc tcgtcagcaa cggcacatcc aactcgaatc tgaccgtgaa cttcaccgtg
    26221 cccgcaagtg ccggcttcgg tcctgccggc gtgcgcgcgc gcctcacgag cacgatgagt
    26281 cccggccctg ccggcgcgtc gggcaatggt gaagtggagg atcatgtgtt gaacgtgaca
    26341 tgccctgcca tcacgttgat tcccgcctca acggcaaatg gcaccgtggg tctggcctac
    26401 tcccaggcac tccatgcgtc gggcggcacc accccgtaca gctacgccat caccaatggc
    26461 acgctgcccg ccggcctctc ccttgacggc agcaccggag tcatcagcgg cacgcccgcc
    26521 gtcgccaatg gtgccggcgc tgctgtcacc atccgcgcca ctgacgccaa caactgccag
    26581 gggtcgcgag tttacacgat gatcgtctgc ccggtcatca cggtaaaccc cgccagcctt
    26641 gccaatggca cggtcaacgc ggcctactcg caggccgtcg gcgctgcggg tggcgcggcg
    26701 tcttacacct actcgctggc atcgggttca cttcccgctg gcctgtcttt gaatgccggc
    26761 actggtgtca tcagcggcac tcccgccagc accagcgccg ccagctttgt cattcgcgcc
    26821 acggatgcca acggctgcgc gggaactcgc agttacaccg tgactccggc ctgcccgacg
    26881 atcaccgtga atccggtgtc gcttgcggat ggcgcggcgg gggtggccta ttcacagacg
    26941 gtcagcgcca cgggtggcat tgcgccttat tcattcgcgg tcagcgcggg cagcctgcca
    27001 gccggcttga gcttgaacgc caccactgga tccatcaccg gaacgcccac cactggcaac
    27061 gccgctggca cgagcgtgac gatccgcgcc acggacgcca acggctgcca ggccacgcgc
    27121 gcttacagca tcaaaatctg cccggtcatc tcagtgaacc ccgccagtct gtccaatccg
    27181 actctgggcg cggcctactc tcagacggtc agcgccagcg gcggggtggc gccttatgtc
    27241 tatgccatca gcggcggcgc gctgccttcg ggcctgagct tgaacaccag cacgggtgcc
    27301 atcaccggca cgaccagcag cactgccagc gccagcttca ccttgcgcgc cacggacgcg
    27361 cacggctgcc agggcacgcg ggtttacacg ctcacgccga gctgtccggt tttcactttg
    27421 aatcccgcca cgctgccgga cgccacgcag gggcaggcct acagccagca gatcaccgtg
    27481 tctggcgcaa atggctcggt ctcctatgcg gtgagcgcgg gcagccttcc tgacggtctc
    27541 acactgaact ccggcaccgg cctgctcagc ggcacgctca gcggcagcgg tgtcttcagc
    27601 ttcaccgtgc gcgccaccga ctcgctcggc tgccagggca cgcgaaatta caccatcaac
    27661 gcggcctgcg cggtcagctc catcctgccc gcctcgctgc cgcacggcct gccgaaccag
    27721 ccttatgagc agcagctcat cgccgacact tccagcggtt tgcgcggcga atactacacg
    27781 gggcggaact tcgagacgct gaccctggtg cgctatgaca cggcggttaa ttttgactgg
    27841 ggaaatggca gcccgcacgc cagcctgcct tcggacaact tcagcgcgcg ctggaccggt
    27901 catgtggtcc cctccgtcac gggcagttac attttccgca ccacctccga tgacggtgtg
    27961 cgcctttggg tgaatggcgt gctcgtcatt gaccagtgga aggaccaggg gccgaccagt
    28021 tacgccgcca cggtggcgct caccgccggg cagccggtga gcgtgcgcat ggactactat
    28081 gaaaacggcg gcggcgcggt ggcgcggctc gaatggcagg ggccgacctt ctcgctcaaa
    28141 ccgctcaccg aatggaccac gaacacctgg aacgtggtgg atggctccct gccgcagggc
    28201 atcgagttga atgccgccac cggtctgctt agcggtatct tgggcacgat caacacctcc
    28261 tccttcaccg tgcgtgtcac cgactggcgc ggctgctccg gcacgaagac ttacagcatc
    28321 caggcggaat gcccggccat cgccatcacg ccggagacgc tgcctccggc cacggtgggc
    28381 gcgccgtatt cccaggctct cgcggccagc ccgccctttg gactcaaggg ggaatattac
    28441 gcgggcatga atttcgaatc gctcgtgctc acccgccagg atgccgccgt gcatttcgag
    28501 tggggtgagg gttcgcctca cgccagcgtg ccggtgaacc agttctccgc gcgctggagc
    28561 ggcttcatca cgccctccac cacgggcagc cacaccttcc gcgtgcgcgt ggacgacggc
    28621 ttccgcctct gggtgaacaa caacctcatc atcaacacct ggaaggacca ggcccccgcc
    28681 gtctatactg ccacggtcaa tctcaccgcc ggcgtcacca tcccggtgcg cgcggagtac
    28741 tatgaaaacg gcggcggcgc caccgcccag ctcgaatggt ccggccccgg attctcgaac
    28801 cagccgatca cccagtggac cggctacacc tggagtctgg ccaatggcac gctgcccgcc
    28861 ggattgaccc tgaatccagg cacgggcgtc atcagcggca cgcccacgac gacttccagc
    28921 ggcaccttca tcgtgcgcgc caccgacccg aatggatgct ccgccacgcg cagctacacg
    28981 ctcggcccgg cctgccctcc gctgacgatc ctgccggaga ctctggccga tccttatctt
    29041 ggcgttgccc acagccgcca gcttcaggcg gatggcgcga cgacggcgat gacctggagc
    29101 gtgacctccg gcgctttgcc cgcaggtttg agtctgagca gcgcgggcct gctgaccggc
    29161 acgccggcgc aaaccggcgc cttctccttc accgtgcgcg ccaccgacgg cggggcttgc
    29221 tcggccacgc gcacttactc gggcgcgacg cgttccatgg gcatcggcaa ccaagtctgg
    29281 gccgacatga acaacgacgg cgtccgccag tcctcggaga gcggcattcc cggcctgcgg
    29341 ctggaactgt ggtcgcccgg cgccaacggc gtgcgcgaga acggcggcgg cgatgatttc
    29401 aaagtcacgg cggatgtgtt caccgacgcc agcgggcagt atcaattccc cctgctcacg
    29461 ccgggcgtgt attacatccg catcccgaac ccgccggcct actatccggc tgtgagcgcc
    29521 aacggtgtcg cgctcgacaa cggcgtgaac aatgacagca acggcaccca gcccggcggc
    29581 tccggcacgg cggtggtcag ccctctcatc acgctcagtc ccggcgcgga gcctggcgtg
    29641 gcggtggatg gtgatgacac ggacaccgac tccacggtgg acttcggctt tgccaatccg
    29701 gatgtctgct acgcggccaa tctttttgac aatccgagct tcgaattcca gcagctcccg
    29761 aacagcaccg gcacgcccgc caccgtgctc ggctacaacg gcaccggcac aggcctcggc
    29821 acgggcatca acgccttcca atgggtgggc ggcatcaaca gcgcgagcgg ccttggcgag
    29881 ccggtgcagc gtgtgcaatt gagcgcgggc agcgctggtg ctcgtgtgtc atggatggaa
    29941 agcctgaagt cccgccacgg tcgtcgtcat ctgctgctcc agggcaccaa cgccacggcc
    30001 agcctgcgcg ccgcgggtgg tggtgactgg tccaccgtgc tgcaagccgg gcgcgagtat
    30061 cagctctccg tctgggcggc gaatgccagc gacgccacag catccctgct ttggaatgtc
    30121 acggccaatg cctccatctt ccaggtcatc accgggccga ctccgggcct ttatcaaaac
    30181 tacaccgtgc cgcaggcgga gatgaccgcg agcctgccgg gccagcagca gtgctgcggc
    30241 aacagcttca ccggcacgag catggcctcg ttcgccccgg cggattacaa cggctggtcc
    30301 gaggccgtgg aaaacggcgc gcaacccgtg tggagacagt tcacctggcg cttccgcgtc
    30361 acacccacgg ccaccgccac tcagatcaac accgccggtt tcggcatcgg tgccggctcc
    30421 ggcagcggcc cggtggtggt ggactatgtc tcgctctgcc aggtggacgc ctcgaacacg
    30481 ctcacgcttg gcaacctggt ctggaacgac ctcaacaaca acggcgcgcg cgacggcgcc
    30541 gaactcggcg tcggcggtgt cagcgtgcgc cttttcacca ccaccaacaa tgtggccggc
    30601 gacggcgatg acgtgctgct cgccaccacc acgacctccg tggccggagc ctacaacttc
    30661 accaacctcg cccccggcaa atacgtcatc cgcttcacgc cgcccgcgca tctgcccctc
    30721 accgggggcg tgcccgcgac cacggacaac cacgtcaaca accagaacaa aggctcccaa
    30781 cccggcggac ccggcaccga cttgatcagc cctgtcatca tactggccct cggcgcgggt
    30841 ccggtgaacg acggcgacac ggaccccgac accaacctca cgctcgactt cggtctgtgg
    30901 agcggcttca ccctcggcaa tcaaatctgg gccgacacga acagcgacgg cattttccag
    30961 agcggcagtg aaagcggcat cgccggtgtg acggtgcagt tgatggacga gacggccgcg
    31021 agcgtgctcg ccacgaccac gacgaacatt tcgggcattt acagcttcac acgccaccag
    31081 gccggcaaat atcgcatccg catccccgcg ccgcccgcgt ccttcccgct catcaccgcc
    31141 gtcaatgtgc tgctcgacaa cggcgtggac catgacagca acggcagcca gcccggcggt
    31201 gtcggcacgg cggtgctcag tccggtcatc acactcgccg ccgccaccga gcccggctcc
    31261 tccggcacga cgaacataga caacacgctg gacttcggct tccgcgcctg cccggccatt
    31321 gccatcagtc ccgcgctgct gaccgcagcg cggcagttca gcgcctattc ggtgaatctc
    31381 agcgccaccg gcggactcgc gccctacacc tggaccgtga gcaccggcgc gctgccggac
    31441 ggcttgagcc tgggcgccgg tggtttgctc tccggtacgc ttggagcggg cgcggtgccg
    31501 ggactttaca atttcaccgc gcgcgccgtg gacgccaacg gctgccaggt cgagcgtgcc
    31561 tgctcgctga caatgcagcc gccgctcatc ggcatctcac cgcctgcgct gcccgccggc
    31621 gcgcaatacg cgccctactc gcagcaactc tcagccagcg gtggcagcgc gccctactcc
    31681 tggagcgtga gtccggcgct gccgtccggc gcagtggctt ggtggatcgc ggaaaatggc
    31741 gcgggtgacg cgcttggcag tcaccacggt ctgggcctga atggcctggg ctacacgaac
    31801 ggcccggtgg gccgcgcctt caacttcgat ggagtggatg acgtggcgca ggtggaggac
    31861 gccgccgctt tgaagccggc gcagatcacg ctcgaagcct gggtcaaccc ggccgcaagc
    31921 ggcctgcccg ccaacggcgt gatcctcgcc aagagcgcgg gttccaacgg ttacggactc
    31981 ggcttgaaca gcggcgcgga caacctggcc ttctggatca atgaccgtgc gggcaactcc
    32041 atcaccgccg ctttgactcc gggtgtgtgg tcgcatgtca ccgccaccta cgatggcacg
    32101 gcaatgcggc tttatgtcaa tggcggcctg gtggcttcca gcgcttacag cacaaccatc
    32161 acccacagca gcgagccgct gcgcatcggc aacctcggcg gcggcgcggc ggcatggaag
    32221 ggcggcattg acgaggcggc gctctacaac cgcgcgctca ccgcgcagga gatcgcggcg
    32281 cgttacctcg gcacccagat gggcaaccag ggcatgccgg aaggtctcgc gctcgatccc
    32341 gccaccggcc tgctctccgg cacaccgacc ggcgtgccgg acacccacca cttcgtcgcg
    32401 cgcgccagcg atgcgaacgg cagccccggc gtgcgcgctt acgctttgga gatcatctgc
    32461 ccgtccatca gcatctcccc cggatccctc gccagcacgg agaagggagc cgcctattcg
    32521 gcggaattga acgccgccgg cggcaccgga ccctatgcct ggagcctgtt ctccggcagc
    32581 ctgccggtgg gggtgactct cggcaccgcg ggcatgatct ccggcactcc cggcgccttg
    32641 gggaccttca acttcaccgt gctggcgcgc gacgcgaaca actgctccgc cacgcgcgtt
    32701 tacacgctca cggtggaatg ccccgtcatc gaactggccg gcgacgcgct gcccgaggcg
    32761 cgccagttcg aggcttacac tccgagcgcg cttggagtga ctggcggcga ggcgccgcac
    32821 agctttgcca tcgtcagcgg cgcgctgccc gccggcatga gcctgagcgc agagggcgtc
    32881 atcagcggca cgccttccgc cgtgccgggt gtttacagct tcaccgtgcg cgccacggac
    32941 gcggcggact gcgccggcac gcgcgagcat cagatcaccg tgggctgccc ggtcatcgcc
    33001 atcagcccgt ccacactgcc gcaggccgcg cgtttcgccg cttattcgca ggcgctcgcc
    33061 gcctcgaacg gcaacggcgg ttacgcctgg actgtcagcg ccggcgcgct tcccgatggc
    33121 ctggcgatga gcggttcggg tgtcatcagc ggcacgccga cggcggtgcc gggagactac
    33181 ggcttcaccg cgcgcgtcgt ggatgcccag ggctgcgccg ccacccgcgc ttacaccctg
    33241 accatcggct gcccggtcat cgccatcacg ccggccatgc tgccggacgg cacggccacc
    33301 gccgcttaca gccaggctct caccgccacg ggcggcggcg cgccttacac ctggtcggtg
    33361 gaaggcggca gcctgcccgc gggcatcacg ctctccccag gtggcgtgct ctccggcacg
    33421 acggactcgg tgggcacctt caacttcacc gcgcgcgcgc tcgacgcgca gaactgcgcc
    33481 gccaccagaa gctactcgct ggtcatcggc tgcccggtca tcgccatcac gccggagagc
    33541 gtgcctgacg ccaccaccgg cgcggcttac tcgcagacgc tcaccgccac gggcggcgtc
    33601 ggctccatca cctggagcat ggtcagcggc agcctgccgg ctggagtcac attgagcgcg
    33661 gcgggtgtgg tcagcggcac gccttcagga gcgccggggg attacaattt caccgcgcgc
    33721 gcgaccgacg cgaacaactg ccaggccacg cgcgcctatg tgctcaagct ggtctgcccg
    33781 gccatcacgg tgacgaacgc cagcctgcca aacggcacga cgagcgcgaa ctacacgcac
    33841 agcctcagcg ccgagggcgg cgccggacct tacacctgga cgctgccaag cggcagcctg
    33901 cccgccggcc tcaccctcaa tggggaaggc gtcatcagcg gcgtgcccac agcggcgggc
    33961 acggccagct tcaccgtgca ggcgcgggat gccttcaact gcaccggcac gcgcgagatc
    34021 acactcaccg tcgaatgcgc cttggtcgca gtcagcacga acagcgtgcc cgcaggttat
    34081 cgcggcgtgg cctacagtca ggcgctcgcg gccagcggcg gcaccgctcc ttatggctgg
    34141 gtgctggaaa gcggcgcgct gcccgccggt gtgacattgg gctccaacgg cacgctttcc
    34201 ggcacgccct ccgcgacggg tgatttctcc ttcaccgtgc gcgccaccga cgcgcatggc
    34261 tgctcgggca cgcgcgcttt ggcgctctcc atcggcggcc ttggattggg cggcctggtc
    34321 tggaatgacc tggatcaaaa cggcgtccgc gacgatggcg agccgggcat tggcagcgtg
    34381 acgctccagc ttttcaggtc ggaaaactct gtcatcggcg atggcgatga cacactcgcg
    34441 gacaccacga ccaccgatgc cggcggctcc tacggcttca gcggtctcgc gccgggcagt
    34501 tactatgcgc gcatccagaa tcctccagcc gggctgccat tctcctccgg tccgcaggtg
    34561 gcggcggaca acggcgtcaa ccatgacaac aatggcctgc aacccggcgg cagcggcacg
    34621 gcggttgcca gcccgctgat tcagctcggt gttggccgcg agccgggtga tcaggcggag
    34681 ggcgcggatg tggatggcac ggtggacttc gctttccgcg ccgtgcccgc gccggatgcg
    34741 gtgctgctcg aatacaatct caatgcttcc tccggcggcc tgccgccagc gccaagcacg
    34801 aagaatcctt ggatcgtcaa cagcgccaag ctgcagatcg aggacggcgt caacggcctg
    34861 gacaatctca ccgagacctc gaacaacggc cccattctgc ctggtgagtt gagccgccgc
    34921 gtgcgtggct ggctggagga tgccgatccg gcgcttgagg agccgcgcac cagtctgaat
    34981 gccgcgcgtg acacggcctg ggtgcgtttt ggcatgggtg ctggcgtgac cagtggcggg
    35041 attgacagcc tgcatctcga catgcgccgt gtggatgcgc tggcacccac gcagggcgtc
    35101 gcttatttga cttggaatga gggcggcact ttccacacag ccgtttcaga catcttcgaa
    35161 gtcgccaccc agggcgcctg gcagtctgtc acggttgact ggaccagctt cctcggcggt
    35221 gccaccgcac tgccttcggg cgcgcagctt gccgggaagt ccttcctttt ggaaatccac
    35281 ctccacggcg gcgatgagag cggctttgtg gacctggaca atgtcattct ggcgggcacg
    35341 gctgatggcg gcacccccac gctggctctc ggcgaccgcg tctggcagga cctcaatggc
    35401 gacggtctgc aaaacgccgg cgagcccgga atgcccggga tgatcgtgca gctctggcgc
    35461 gccggggctg acaatctgcc ggacacgggc gatgacgtgc tgcacgcctc cacggtcacg
    35521 gatgcgctag gttattattt gttcagcgaa ctcgcccccg gcaaatactt cgcgcgcgtc
    35581 ccgacccctg atgacgagtg gccgatgatc tcgccgggca gctcgaatga cgacggtgtg
    35641 gacaatgaca gcaacggcat ccagcccggc ggacccggca cggcggttca cacgccggtc
    35701 attgacctgg ccctcggacg cgagcccggc gaccagcccg atggcggcaa ccaggacctg
    35761 acagtggaca tcggcttcag cgcctcgctc tccctcggca acctggtgtg gggtgacaat
    35821 aacaacaacg gcctggtgga cgcgggtgag ggcggcgtgg cgggcgcgca ggtggagttg
    35881 ttccgctcca ccgacacggt ggtcaacaat ggcgacgatg tgaaggtggg cgcgacccag
    35941 gtcaccgact ccagcggcgc ttatctcttc agcgacctgg gcggcggccg gtattatgtg
    36001 aaggtcacac ctcctgtcac ccacacacgc cgcagcggca cgtcgttcaa cacggataat
    36061 ggggtggaca atgacaacaa cggcatcacc caggccggcg ccggcgcgcc cgtctactcg
    36121 atgatggtga acctctccgc cctcaccgag ccgggcaatc tgctggcgcc cttcggcggc
    36181 aacgcggacc tgacgattga tttcggcctg cgtccggtct tcctcagcgt cggcaacctg
    36241 gtcttcaagg acaccaacgg caacaatgtc catgacgctg gcgagggcgt cggcggtgtg
    36301 cgtgtggagt tgctcaatgg cgacggcgtg tttttgagca gcacgaccac gagcaacgca
    36361 tccagcaacc tgggaggcta ccagttcagc aatgtgctgc ccggccagta ttatgtgcgc
    36421 attcccgccg gcgagtttgg cgcggccaag cccctgcaaa acagcctctc cattagcccg
    36481 gcctcgccgg tggacgacgg cctggatgac aatcatgcca atggtgacag cggcgtggac
    36541 agcgccagcc ccgcgctcac cggcatctcc agcccccgcc tcaccctcgc ggagggcgcc
    36601 cttccgaccg cggcaaccgg agagacgggt ttccgcgcgc atctggacga ctccgccgac
    36661 gcgaacggca acatgaccgt ggacttcggt ttccgcgccg caggcccctc ggcggaaggc
    36721 tgctaccact tcgccttcaa tgacgcggtg cgcgaccccg acggcatcct gcccgcagcc
    36781 tccgcctggc agcccgcgca gccgtatgat ttccaataca gcgccggccc cgcgcatgtg
    36841 gacgcctttg atttgaagtt cgacgccgcc gtcagccgca tcgtgctcga cgccaccttc
    36901 acccagctca gcggacgccg cctcgacgcg ctggccatga tgctctccac cacgggtgat
    36961 ccggcgaatg ccaaccacgc cattctctat ctcgacgcgc acgaccgcgc cgcgccaaag
    37021 atggccgtgt accgctacga tcccgcgctt ggactgcaaa gctggcagaa cagcgccaac
    37081 ctgctcgcca gcagcggcaa cgccaccggc gtggacgtga tgcagaacaa ggtcactgaa
    37141 accggcgcgc aggcgcgctt ccagttcgtg ctcgacatca gccgcgtgaa caacgccgcg
    37201 aactgggccg cgctcggtgt cagcgcgctg acctgggagg gcattctcat gggcggcaac
    37261 gcgggactcg gtctgtggac gctcgatctc gacggcgcgc ccgcctacaa tgcgggcggc
    37321 gcattgaccg ccttcccgca cacctccagc gcggccaccg agggcggctt cgcgccgagc
    37381 gccgggctgg acatcatcac ccagccctgc ccgtcctcgc cctgggtgag catcggcaat
    37441 ttgattttta gcgacacgaa cttcgacggc gtcaagcaga gcaacgagtc cggcatcacc
    37501 ggcgccacgg tgcagctctt cagccccggc gcggacgggg ccatcggcgg cgagggcgtc
    37561 aatgccgaca cgcaggtggg ccaggccatc gtcaccacca gcaacggcgc ctacaacttc
    37621 accaacctca tccccggcaa atactatgtg cgtgtcatgc cgcccgcagg cttcccgctc
    37681 accggcggcg ggccggtgcc gctcgacaat ggcgtcaaca acgacaacaa cggtcatcag
    37741 cccggcggcc ccggcacctt tgcctacagc cccgtcattg acctccagcc gggccaggag
    37801 cctgccgtgg ctgtggatgg agacggcacc aatggcgacg ccaccattga tttcggattc
    37861 tggagcggtt tcagcgtcgg cgacctggtc tggcatgaca tcaacaacaa cggccagcgc
    37921 gacactggcg agccgggcgt ggcgggcatc tttgtcgagc tgatggaccc cggcgcggac
    37981 ggcttgatgg gcggcgcggg cgcgaatgcc gacacggtgg tcgccacgac gaccacgaac
    38041 tccagcggcg cctacgcctt ccgcgtctat caaggcggca aacgctatgc gcgcatcacc
    38101 cccacggcgg ccctctcgct cgccagtgat gcggcggtgc agttggacaa tggcgtgaac
    38161 aacgacagca acggcgtgca gaccggcctg gcgggcagcc ccatagtcag catggtcttt
    38221 gagttgatgc cgcgccgcga gcctggaaca agcggctcca ccaacaacga gctgaccatt
    38281 gacttcggca tcctcggctg cccggtcatc aacatcagcc ccaccgcgct gccgctggtc
    38341 actcatcaga cctcctacag ccagacgctg aatgccgagg gaggcaaggc tccctacacc
    38401 tggagcgtgg tggccggcac gctgccgcag ggcctgactc tgaccagcgg cggcttgctc
    38461 ggcggcgtca ccacggcgct gccggggaac tacagcttca ccatccgcgc ccgtgatctc
    38521 tccggctgcg agagcacgcg ctcctacacc ctcacggttg tctgcccaac gctcgccatc
    38581 acgccgtccg cgctgccgga tggcactcaa ggcgcggctt tcagccagac gctgacagcc
    38641 accggaggca ccgggcctta ttcctgggcg cagacttcgg gcacgctgcc ggcgggtgtc
    38701 acactttccg gcgcgggcgt tttgagcggc acgccgcagg cgccgggagt ttacaacttc
    38761 accgtccgcg ccacggactc gctgacctgc caggtgagcc gggcttatgc gtggacggtg
    38821 caatgcccgc cgctttccat atccccggcc acactgccgg cggccacgct cgacgctgct
    38881 tacacggcgc agactctcac cgcctccgga ggcaccgcgc cttacgcctg gacggtctcc
    38941 gccggcgcgc tgccgcaggg catgggcctc gccgccaatg gcactctgac cggcacgccc
    39001 tccggcgcgc ctggagccta taacttcacc gcgcgcgcca ccgactcgca cggctgcgcc
    39061 gtcacgcgag cctatgtctt gacggtgaat tgcggcgtca tcagcatcgc gcccgccagc
    39121 ctgccggagg cggtgcagtc cgccgtttat gccgcgcaga ccttcaccgc cgcaggcggc
    39181 accgggcctt acacctggag catcagcgcc ggatcgctgc ccgccgggat taccttgaac
    39241 gcggacggcc tgctctcggg cacgccgacc gccgcgccag gcgcctacag cttcactctg
    39301 cgcgtggcgg acgcctataa ctgccaagcc acgcgcgcct acgcgctcca ggttctctgc
    39361 cccgtcatca gcatcacccc ggcctcgctg gcatcggcgg tgcagtttga tgcataccac
    39421 ctgcctttgt cagcttcggg cgggcagggg gcttacgcat ggaccgtgag cgcgggcgcc
    39481 ctgcccaacg gcgtcacact ttccagtgcg ggactgcttt ccggcacgcc gcaggagacg
    39541 ggcgtgttca gcttcaccgc gcgcgccacc gacgggcagg actgctccgc cacccgtgct
    39601 tactcgctca ccgtgctctg cccggtgatc acgatcacgc ccgccgcttt ggaggacgcg
    39661 cccgcgcatg tggcctacag ccggaccttc accgccagcg gcggactggc gccttacacc
    39721 tgggacatcg cctccggcgc gctgccgcag ggcatgaccc tgagcaccgg cggtgtgctc
    39781 ggcggcgcgc cttcgtccgc gccgggagtt tatgagttca ccgtgcgcgc caaagatgcc
    39841 gccgaggcct gctcggccac gcgcgtcgtg tcgctcacgg tcagttgctc ggtggtgcag
    39901 atttccccgg cctcgctggc gaatgccacg gtgggtgcga gctactcggt caatctctcc
    39961 tccaccctgg gcagcgcgcc cttctcttat tcggtggtct ccggctcgct gccgcccggc
    40021 ctcacgctct ccagttccgg cctgctctcc ggcacgccca cgcaggcggg cacggccggc
    40081 tttaccattg aatcccggga cacttttgac tgctcgggca cgcgcgctta caagctgacg
    40141 tcggtctgcc cggtcatctc gatcacaccg tccacgcctc cgccggctta tcacagccat
    40201 gcttacacac agcagctcac ggcctccggc ggcaccgggc cttacacctg ggcgctggtc
    40261 tccggctcgc tgcccgcggg gctttcgttc tcggcggcgg gcgtcatcag tggcacgccg
    40321 acggcctttg gaaccagcat cttcaacgtg cgcgccacgg acgcctacaa ttgccagtcc
    40381 acccaggtca tcaccctgct ggtgaagggc ctcgccatcg gtgacacggt ctatgatgac
    40441 gccaacttca acggccagcg tgaccctggg gaaagcggcg tgcctggcgt gacggtgcag
    40501 ctttgggatc ccggcgcgga caacgccatc ggcggcaccg gcgcgaacag tgactcgcac
    40561 atttcctcca ccgtcaccga cgcgcagggc cgccacactt tcaccaatct gctgccgggt
    40621 cgttactatg cgcgcgtgct gccgcccgcc cacctgccaa tgcgcggcgg caatccggtg
    40681 agcctggaca acggcgtgga caacgacaac aacgccgcgc tccagcccgg cggccccggc
    40741 accgccatct tcagccctgt catcgagctg gccatcggag ccgagccgct ggcgggcgat
    40801 ggcgatcccg acacggacat gaccgtggac ttcggccttt atcgcggact caagctgggc
    40861 aacctggtct gggaggacag caatgacaac ggcctgcgcg atgagggaga gccgggcatc
    40921 aacggcgtga ctgtccaact ctggagcacg ggcgcggacg gccgcatcgg cggcgtggat
    40981 gacacgctgc tgggcacgac ctccagcgcg ggcgggggcg cgtatctctt cgagcatctg
    41041 actccaggag cctgctatgc gcgcatcccc gcgccgccgc aaagccatcc tctggtcagc
    41101 aaggtggcga atttgcgcga tgacggcgtg gaccttgaca acaacggcca tcaaatctcc
    41161 ggcggtgcga tttactcgcc ggtcatcacc ctcacctccg caggcgagcc tggaaacagc
    41221 ggctccacct atgaggagct gacggtggac ttcggcctcg tcaatgtcac gcccagcgcc
    41281 tatgtctccg ccacgcatga cgacggcatt cacgctttcg atccaggcac cggagcattt
    41341 gccggtgttt tccatcatcc gtttggcgac agccacaacc agggcgacgg caatcccttc
    41401 gacgtgccct atgacattga gacaggcccg gacgggcact tctatgtggc gcacttcggc
    41461 gcgggcaatg tccgccgcat cagccttgcg ggcgcggaca tgggcgcggc gctgaacacc
    41521 gccgccgcca atgtcacact ggtgaagcgc tttgccatcg ggccggacgg caacttctat
    41581 gtggtggaca tcaacaccag ccgcatggtg cgtttccacg gccctctgag cgacacgccc
    41641 ggccagccgc tcgatcccgc gcctcacacc ttccttcagc agcccggcat cgaggacatc
    41701 aacttcggcc ccgacggcaa tctctacctc gtcatccagg acggcttcaa ccgcgaggtg
    41761 cgccgccaca gcgccaccac gggcgcatat ctcaacagtg tgatggatga catggcgctg
    41821 gtgaacctcg tgccgggagg gcagccgacc gccatcatct ccggcgtgga tttccacggc
    41881 tccaccctct acggcatcaa ccgcaccgag ggcgagatct tccgcgtgga cctctccaac
    41941 cccgccgctc ccggccagcc ggagctggtg gccgtgatcg gcagcgccgg tttcggcttc
    42001 atcgagacgc gcgacctgga ggtggacccg cgcaccggtc tgctgcacat caccggctac
    42061 cactggagca aacccatcct cggcggcaac tacaccagcg ccgccatgct gcgcgtgaac
    42121 cccgccggcg cgccgaatgc cgcggtggaa atcttcgagg cgccgatgcc atcgcctccc
    42181 ggtccgaaca atgaaatctg gtccggcccc tcggcgctgg catttgccag gccgttcgcc
    42241 agcctgggcg agaccgtctc catcggcagc ctggtgtgga atgacagcaa cgccaacggc
    42301 ctgcgcgacc ccggcgagct gggcatcccc ggcgtgcgcg tggagctctg gcatgacgcc
    42361 gacgggaacg aaagcaatgg agcggaggtc cgcatcggct ggaccttcac cgacgcgcgc
    42421 ggccattact acttctccgg ccagaaaccc ggccgctacc aggtgcgcgt ttccctcatc
    42481 aacttcggcg acgggatgcc gctctccgga tcgggcatca gcagtccggt gtcctcgacc
    42541 ttggacgatc aaacggatga tgacgacaac ggcagccagc ctggcggcgc gcgcacggaa
    42601 gtcttcagcc cgctcatcac gctcacgccc ggccaggagc cgctgggcaa tgcggtcact
    42661 ggtgtggaaa ccggctccgg cggcgacctc gatgacttca ccaccgatgc gaacggcgac
    42721 atgaccgtgg acttcggatt tgtcgaaacc ggcagcatgg gcctcggcaa cctggtcttc
    42781 cttgatgcca acgacaacgg ccgccacgac acgggcgagg gcattgatgg ggccttggtc
    42841 gagctttact tctggggcat gacgcccgga ctcgacccgc cgatccgctc caccgtcacc
    42901 gccaatggcg ggcgctacct ctttgccgag ctgtggcagg gccaatactt cgtgcatctg
    42961 cccgcggcgc agttcctctc cacaggcgtc ctgcgcggac tcttcagcct gcccggagtg
    43021 caggcgggtg atgacaacct gggcgaggac ggcctcgatg acaccgaccc gcccacgcag
    43081 ggcatcagca ccggccgcgt catcctcacc aaggacagca ctcccaccaa tgccaccagc
    43141 gagaccggct ttgaccacac ctccgacgac gacgacgacg ccaacacgga cctcaccatt
    43201 gacttcggcc tctataacgc ggtggccgcg ggcaacatgg tcttcatgga cgccaactcg
    43261 aacgggcgct acgacgacgg cgaggggctc gacggcgtca cactggagct ttaccagttc
    43321 acccagacac ccggcgagga cacgcccgtc gtcaccaccg tcagcgccaa tggcgggcgc
    43381 tatctcttcg acaagctccg gcccggaaac taccgcgtcc acattcccgc ctccatgttc
    43441 gcgaccggcg cgccgctctt ccagcgtgtc agcatccccg agggcctgct cggtgacgat
    43501 gacgcgggcg aggacggcct caatgagggc gaccccgcgc tcatcggcgt cagctccggt
    43561 cttatcgcgc tattccccgg ctcatgcccc gcggatgaaa gcggcgagac cggcgtggac
    43621 cacgccaccg acaatgaaaa cgacgccgcc gtggacctca cagtggactt cggcttccag
    43681 actccggtgg gcgtgggcaa cctggtcttc attgacgcca acggcaacgg acgcgccgat
    43741 cccggcgagg gcgtggatgg cgtgaaggtg gagctttacg caggcgacca gaatccaggc
    43801 gtggatcaat ctctgttcag ccagctcacc gcggatggcg gcttctactt cttcgacttt
    43861 ctcgcctccg gcgactacat cgtccacatc cccgccagcg agttcgcgcc gggccgcgcg
    43921 ctgcacaaca ccctctccat gtccggcgcg cagacggaca actcggacga caacctcgga
    43981 gagggcggcc tggacaacgc cagccccgcg ctcaacggca tctccagccg cgtcatctcc
    44041 ctcgcggtca atgccgaacc tgtcaacgag cccggcctcg gcggcaacat ggacgccttt
    44101 gatgacaaca acttcaacct caccgtggac ctcggctttc tgccggtgaa cgccaacgcc
    44161 gtcggcgtcg gcaacctcgt cttccgcgat ctcaacggca acggacgcca cgaccccggc
    44221 gagggcgtgg atggcgtgac cgtgcggctt ttcaacggca tggccgaccc gctcaccgcc
    44281 agtcccctgg ccaccaccat cacggccaat gagggtatct atttgttcag caacctggcc
    44341 gcgggcgact acatcgtcca catcccgccc tccgagttcg tgtcaggcaa gcctctgcac
    44401 ggctgggtgt ccgtgcccgg ccagggcggc gacaacggcc ttgatgacaa tgtggacgag
    44461 aacggcagtg atggcgatcc gctcaccggg ggcgtcagct ccgtggtcat tgccctggct
    44521 cccggcgccg agccggtgaa cgcttttggc gagaccgggc gcgatgcgtt catggacgat
    44581 gccagcgacg cgaatgtgga cctcaccgtt gacctcggct tccacaaacc ggtggccgtg
    44641 ggcaacctcg tcttcattga cgccaacaaa aacggtgtgg ctgatgccgg tgaaggcgcg
    44701 gctggcgtgg aggtgaatct ttacgccgag ggcatggacc cgatgttcga cccgccggtc
    44761 ggcagcgcca ccaccgatgc caacggcttc tacctcatca gtgatctggc ctccggcagc
    44821 tacttcctgc atgtgcctta cacgcagttc ttgttcggcc agcccctgca cggcctcgtc
    44881 agcgtcgcgg gcgccgccgc cggcgatgac aacgcgggtg aaaaaggcct cgacgacgcg
    44941 cggcccgatc tcaatggcat ccgcaccgcc gttttcaacc tcgtccccgg ccagtgcccg
    45001 gtgagcgagc ccggactcgg cggcgacagc gacgccggca gcgatgaaag caccgacctc
    45061 acacgcgact tcggctttgt gcagccggtg cagatcggca atctcgtctt ccgcgatgac
    45121 aacggcgacg gcatcttcga tcccgacttc gaatacggcg tggaaggcgt caccgtgcag
    45181 ctttggtcga acagtccggc ggtcaagctg gctgagacca tcacctgggg cggcctctac
    45241 agcttcaccg tcgcgcccgg ctcctaccat ttgcgcgtgc ccgcctccat gttcgccagc
    45301 ggcggcccgc tggagggcct gacgccatcc gccgccacac cgaatgacgg cggccagttc
    45361 ttcgatgacg acgccgggca ggatgcctac atcaacggct ccaacctctt caccagtggc
    45421 gcgcgcaccg ccgccttcac catcgcccac ggccaggctc ccggcgaggc cgaaggcgag
    45481 accggcttcc tttattggga ggatgaccac atggaaaacg actccgacct caccattgac
    45541 ctcggcttca tgtctgttga cgcttccgca ggcgcggccc cgcctgatga gccgtcaggg
    45601 atgatgaacc agggcggcat ttccacagac tccaccttca ccgcctggca ggcggagcat
    45661 gagggcatag gcgcgccggg tgacgacccg gatggcgacg gcttccccaa cctgctcgaa
    45721 tacgccctgg ccaccgatcc gaactcgggc atccagcccg cgcgcttcac gctctccgtg
    45781 gatgccgcca ctgcccgcgt ggacgccgtg ctcacgcgcc ccagcggcgg cacgcgggat
    45841 ctgcgctact tcctggaggg ccgcaccgtg ctcgacagcg aatggtccgc gctcccggcc
    45901 agccccgtca tcacgcaaaa caacaacggc accgaaaccg cgcgcttcca ggatgtcgcc
    45961 gccgccacag gccccggcta cctgcgcgtc aaagtcgcgc tcgatgccga cctcgacggc
    46021 acacccgaag ccacagcgct cacacccgcg caggtctggc tgcggcgcga cttcgccgcg
    46081 cagcagacct tctccatgcc gctccagcgc cccgacctct tccggggaat ggtctatgat
    46141 gccagcaacg tcaccctggt catcaacgcc gtcggcctca ccaccaatct tcctgaaaac
    46201 accccgcttt acctcgaagt gctcaccggc gcgcatgccg gccaccgctt cgaagtggat
    46261 gaaagcgcca ccaccgacag cgccatcgtg ctcgaccccg ccgcccctgg cagcacgctc
    46321 ggcaccgtgc ccgcgcttgg cggcgcgcgc atcgccgtgc gaccgcactg gaccctcggc
    46381 gcgctcttcc cggcggagcg cttcacgccc ggcaccagct ccgcggatgc cgaccgcctg
    46441 ctcctccatg acgcggcggc gaatgaattc cgcgtcgtct ggctcggcgc tgacggctgg
    46501 cagggcgaag gtgatgccac ccgcgtcatc cagcccggcg agggcctgct cgtccatgcg
    46561 cgccacgctc cggtgagcct tactttcacc ggccagcttc gcgagggacc cttcatccac
    46621 cgcctcgacg gcctcacccg cctcatcggc agcggccagc ccgccgcgcg cagcccggcg
    46681 gacctcggcc tcaccaccgc cgccggtttc cccgccgcca ccacctctga ccaagccgcc
    46741 cgccttcgcc tctggctcgg cgatacctcg cccggagaca cgggttatga aaacctctat
    46801 ctgcacgaca ccggcagcgg tccgcgctgg attctggaaa acgaccctgc ccaggccgac
    46861 cagaccctgc agcccctcct cggccccgcc cgcgccctct tcctcgtccc gcaggcgccg
    46921 gtgtccattc tcaaagagtg acaccaagtc actgccgggt cattttcccg gcccgtattt
    46981 ttcgaggaac tggttgcgga agatgttgcc ggggtccagg cggcgcttgg tttcaaagaa
    47041 gtcagccgcc atcgggtagg cccgcgcgaa ttgttcgcga gtggcgtggg ggcggtaagt
    47101 caggtagtag gtgccgccgc aggcgagggc ctcctcgatc atgtcctggc tgaaagcgcg
    47161 catccgctca tcggactcgg ggccgagttc catgtgtaaa agcatcacca ggccaaagac
    47221 atgctcgcgg gcgtagggca gcgcggcggt ctcatcgggc ttcacatcgc gcacggtgat
    47281 gttcagcagg tccacaggcc ggtgcttttc aaagagcgga cggatgcggg tgatgaactc
    47341 ccccagtctc ctcaccggca cgaagtactc ctgcagaatc tcggtgctgt ccgtgcggcg
    47401 gctgccgaag tcggaggctg gctcgccctg gatcacgctg cgccatccgc cgtcgagttc
    47461 gccgccgaga cgctcacccc actcccggac ttgctttccg aacccgctgc ccacgctgcc
    47521 gcggaagacg agccgtttaa gcagcggcat gaacccggcg ggtcgtgtct tgggcggtga
    47581 ctcgccggag agccgaagca cattcaccgt gccgctttcc aggaaaccca gcggcgccac
    47641 gttgatgcgc ccgtaggcca ttccggcgga gtcatccaat gtctcgcatt gaaaggtctt
    47701 ccaatagctc tccggtgtca cagccaccgt tttcgtcttg taatgcgcgt ccttcaccac
    47761 gcgcaaagtt gcctgggtga tgatgccgaa gagtccgtaa ccgccaagca catggcggaa
    47821 aagttcgccg ttcgtgtgcg catcacaggc cgcgaccccg ccgtcagcgg tgaccacccg
    47881 cagcgagacg acactgctgg cgatgggtgg caggccatgc tgccagccgt ggcagttcac
    47941 cgagagcgat ccgccgacgg tgaagtcatt gttggactgc atgatctgaa ccgccagtcc
    48001 ggcagcctgc agacggggca gcacgtcccg ccacaaggcc ccggctccga cggtgacaaa
    48061 atgcgcccca ccctcctggc gcggttcatc cacgaaattc agcggagtca tgtccaccgc
    48121 gaccgcgcca tccgccatcg tgtggccgcc catcgagtgc ctggcgcctg cgatggcgat
    48181 gcggccaccc tcagcccgcg cctgccgcac cagtccggcg agctgcgcct ccgcttccgg
    48241 caatgtccgc gccacccgca ccacgcgcgc gggattcgcc aggctgaagc ggctcacgtc
    48301 ctctttgccg ggttcgggcg cgggaggcgc ggcggcggga tcactccacc agaccagggc
    48361 cagatgcccg gcgaggattc cggagagaac gatcacgagc aacaagaggc tggtcaacag
    48421 ccggcgaagc attttcatca ccgaccgttt aacacggctg ccccggcatt tgaaagcgcg
    48481 gtttttttga cgaccgaaat tcccggccct cagcggagca ttggcccgct cagacggtgc
    48541 cgaagatggt cctgccggtg ctcagcaggt cgtcgcaggc ctccttcatg cgctcgcaga
    48601 gaccctgctc gccttttttg aggtagctgc gcgggtcgta agtcttcttg tcgccgactt
    48661 cgccgtctat cttgagcacg ccggcgtagt tcttcatcat gtggtccacg atggggcgcg
    48721 tgaaggcgta ctgggtgtcg gtgtctatgt tcatcttgat gacgccgtag ccgagggtct
    48781 cgcggatttc ctccaacggt gtgccgctgc cgccgtggaa gacgagatcc atttccgccg
    48841 ccgcgccgtg cttgtccatg acggccttct gcccgtcgcg caaaatggcg ggcttgagct
    48901 tcacggcgcc tggcttgtag ctgccgtgga cgttgccgaa agtggcggcg aacatgaagc
    48961 ggccgatgcc gttgagggtc tcatagacgg cgagcatgtc ctctggagtg gtgtagagct
    49021 tttcattggc cacaccggag gtgtcgtggc cgtcctcctc gcctccgaca acgccggctt
    49081 cgatttcaag gatgatgtcg agctccgcgc attgctggag cagttccttg gagacgcgca
    49141 tgttttcctc caggggaagc tcggagccgt caaacatgtg ggagttgaaa aggttgccct
    49201 tgtcggcctc gcggcgcgcc ttggtggcag cgagcagggg tttcaagaat ttctcgacgt
    49261 tcttggggtg gcaatggtcg gtgtggaggg cgacgaggac gctgtagcgt tccgcgagga
    49321 tgtgcgtggc ctcggcgagc acgatggcgc cgagtgccat gtccttgacc gcagtgcccg
    49381 atgcaaactc gcccccgcct gtggacacct ggatgatgcc gtcggatttc gactccgcga
    49441 aggccttcag cgcgccgttg atggtgggca gggaggtgac gttgatggcc gggtaggcgt
    49501 agccgccttt ctgggcggcg tcgagcatgg cgcggtattg ggcgggagtg gcgacgggca
    49561 ttttgttcgg tgttcggagt tagggcgtga ttgggagcaa gaatgatgcc cggcgcaagg
    49621 ctgggttcca ttttgcgcct tgcgcgccat ggggtttccg cgcattcatg gcccgcgcca
    49681 cgcacggata gctcagtggt agagcggctc gtttacaccg agttggtcgg gggttcgaat
    49741 ccctctccgt gtaccaggag cggcagcacc attcagagca gttttttctt tttgaagaac
    49801 cagatcggtc cgagtgcgga gaggatgagt aaaacaatga ccgctggata agcccagggc
    49861 cacttcgtct cgggcatgtg ctcgaaattc atgccgtaga tgctggcgat gagagtgggc
    49921 ggcaggaaga gcacgctggc gatggtgaag atcttgatga tctggttctg ctggatgttg
    49981 atgaggccga gcgtggactc gagcaggaaa gtcatctcct gctgctgctg gttcgtgtaa
    50041 tcatcgagag acttcacgtc ccgcatcaca ctgcggaact gggccgcgag gtcgccgcgc
    50101 agccaggtgg cggcgttgtt gaggaagaac tggagcatgc ggttcacgct caccaggctc
    50161 tcgcgcaggt tcgccaccag ggcgctgcgg cggccgaggg tgcgcaccac gtcgcccagg
    50221 tctttttcga tggccgcgtt cgcctcgtgc gggcgcaggc tgaagatctc gcggcccact
    50281 tttttcaggt cggactccac ggtctggagc gcgtccgcga tgcgcgccac gatgagctcg
    50341 cagagggtga gaaagaccgc gtcgctcgtg gtgggcaggg aacactggcg gcgcacggtg
    50401 tccgccagca ggcggaaggc catcgggtcc gcgtagcgca cggtggtgag gcagtccggc
    50461 gcgatgacga aggagatggc cgtggtgtcc gggtcgggcg tgtccaggcc cacgggaatc
    50521 cacgcggtca tgtagagcgc gcccttttcc atgtagagcc ggctggattc ctcgatctcc
    50581 tgcatctcct cgcgggtggg gagctggtat tcgagccagc tctccgactc cagctcctcg
    50641 gcgcgggaag ggttcatcag atcgagaaag acaagattgt ccgggaacgg acgcactttc
    50701 tcgtcgttcc agtcaatgag ctgtccttgc tgcgtgatga ggcggatcat tcgggggagg
    50761 gtgcgcagtc tagcgccgct cgtccatgcc gccacggaaa agggggtgag tggtgaaaat
    50821 ggcggcgcaa actgtcttgc caccgcgcgc cgcgcccgtt attcactgcg cgcatgtccc
    50881 ttcacgcctg gttccatgtt gacaatgagg ccgagatcgc ctcccccgcg ctgctgcttt
    50941 accgcccgcg catcgagcac aatctgcgcc tcatgctgga gactgccggc gggccggcgc
    51001 ggctgcgtcc gcatgtgaag acgcacaagc tgctgccgct cacccgccgc caggtggagc
    51061 tgggcatcac gaagttcaag acctccacca tcgccgagac cgagatgtgc gcggacgcgg
    51121 gcgcggccga tgtcatgctc gccatgacct gcgtggggcc gaacgcgcac cgcctcgcgc
    51181 gcctggcgga aaagcacccc ggcgtgcagt tctccgccat cgccgatgac gaggagagca
    51241 tccgcactct ggcctccgcc gcgcagcagc accgcgtcac cctcggcgtt tttctggagc
    51301 tggactgcgg catggggcgc accggcatcg tgcccggcga ggccgccgct tttctcgttc
    51361 atgtcatcaa agacaccccc ggcctcatct tccgcggcct gcacgcttac gacgggcaca
    51421 ttcacgatgc cgagctggcc gtgcgccggg agcgcgcgga ggccgccttc gcgcccgtgc
    51481 tcgccttccg ccagcgcctg gagggggagg gcgcgcatgt ggcggagctc gtggccggcg
    51541 gctcgcccac ctttggttat catgccgggc acgccgaccg catttgcagc cccggcacca
    51601 ccgtgctgtg ggattttggt tacggtgaaa ggcaccccga cctggccttc ctgcccgctg
    51661 cagttctgct cgcgcgcgtc atcagcaagc cgggcaagga ccgtctcacg cttgatctag
    51721 gccacaaatc cgtggcgccc gaaaaccccc acccccgcgt gaaattcgag gagctgccgg
    51781 aggccgaggt gctcatgcag agcgaggagc acctcgtgct tcacaccgag cgcgcgcacg
    51841 agtttctcat cggccaggct ctgcacggca tcccccgcca cgtctgcccc accgtctcga
    51901 tgcacggcga ggccgtcgtc attgaccggg gcagggccgt cgagacgtgg cccgtcaccg
    51961 cgcgcaaccg gcggatcacg gtgtagacgg cccgtagtcc gggcactcaa acccgcgccg
    52021 cccgtggaaa ctggacccgc gcttgcaaag cgcctccgcg ccgggatggt gccgcgccat
    52081 gcgcctgctg catctctaca tctctccaga acacaactat tacggtcatc atggacagcc
    52141 tgcgggtacg gctcccatgc tcgaagtgga cgaggtggag tgcgtggcgg gaaagggtct
    52201 tcgtggcgac cgcttttttg gctacaaaga ggattacaaa ggccaggtca ccttcttcga
    52261 gcacgaagtt tatgagcggc tgtgcgcgca gtttggcatt catgacctgc cgccatccgt
    52321 cttcaggcgc aacatcatca ccacgggagc ggatttgaac gagctcatcg gaggggagtt
    52381 cgaggtgcag ggagtgcgct ttatcggcac gcaggaaagc gcgccctgcc actggatgaa
    52441 ccaggccttt gccgagggcg cggaagacgc gttaaaagga catggcggtt tgcgcgcgcg
    52501 cattttgagc gacggcatcc tgcgccgaga gtgacgcccc ggcagcggca agcgtaaacc
    52561 tcacactgcc atgccacgcc gctcattcac cttccccatc gtcatcatcc tcctgcccgc
    52621 cgcgctggcc gcatggctgc tggcaacgga ccatgtggcg cgctcgctgg gcgtggtgct
    52681 gctgagtctt ctggtggtgc tgctgctttg cctttggtgg gcgctgggag cgcgggggcg
    52741 gcgtttgaaa cgacttgcgc tcgtcctgct cgcgggtgcc gcgctgtggg gcgcgggcag
    52801 gctgctgctg cgctacgaag gctccgccga cggcacagcc atgccgcgtt tcgcctggcg
    52861 ctggacggtg tccgcatccg ccgctccggc accgcaagcc aaagccgccg gtctcgacct
    52921 ggccgaaatc cccgccggac tcgccgacat gcgcgccttc atgggagcga gcggcgacgg
    52981 catcttcccc gcgcccgcct ggaagaccga ctgggccgcg catccgccgc gcgaggcatg
    53041 gcgg
//
LOCUS       DAOMYJ010000004        52096 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Prosthecobacter sp. isolate AMR_MDS_5629
            NODE_254_length_52096_cov_21.246959, whole genome shotgun sequence.
ACCESSION   DAOMYJ010000004 DAOMYJ010000000
VERSION     DAOMYJ010000004.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867850
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prosthecobacter sp. (wastewater metagenome)
  ORGANISM  Prosthecobacter sp.
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Verrucomicrobiaceae; Prosthecobacter.
REFERENCE   1  (bases 1 to 52096)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 52096)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.30
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:34:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,742
            CDSs (total)                      :: 4,684
            Genes (coding)                    :: 4,676
            CDSs (with protein)               :: 4,676
            Genes (RNA)                       :: 58
            tRNAs                             :: 55
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..52096
                     /organism="Prosthecobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_254_length_52096_cov_21.246959"
                     /isolate="AMR_MDS_5629"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1965333"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..610
                     /locus_tag="PLP58_00770"
     CDS             <1..610
                     /locus_tag="PLP58_00770"
                     /inference="COORDINATES: protein motif:HMM:NF014640.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HRJ07082.1"
                     /translation="IADFAADLRAPTPSAAAELLAPEAAGLLRHFETRERGMENRVEN
                     LLEHHDRVLDLTARGALARGPEWQLLQSVQQADEAESRLNAGARAQIQELADQTARCQ
                     HTLARRHPEILLSEAGHKVETAGRGMNHLAARLLERIQQRLDSRRDLLRQLGPHAVLA
                     RGFSYTTDSSGRVLKNAADAGDGELIRTLLAEGCIESVVRKK"
     gene            768..2297
                     /gene="trpE"
                     /locus_tag="PLP58_00775"
     CDS             768..2297
                     /gene="trpE"
                     /locus_tag="PLP58_00775"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009964428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004049 - anthranilate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate synthase component I"
                     /protein_id="HRJ07083.1"
                     /translation="MRAFSRMSAPHLQPDPDSFRALARKGNLVPVVAELTADYETPLS
                     AFQKLHDGRCGFLLESAELTQHSGRYSLLGSSPRMIFTARGREIEIEERGRTRAFTTD
                     SDPLDELKKLMRPYRPVESHPIPVFHGGAVGYLAYDMVRFFEPTLAPPPKDDLGLPDM
                     VFMITDTVVIFDHRTRRMMLVANVHTDEHENLDAAYDWARAQISLLIQKLSQPLAVKP
                     LQTIAPISNDSLPPPAGNTTREEYEGMVVKMKEYIRAGDIFQGVPSQRFETAYTGTPL
                     DLYRALRHVNPSPYMFCLQFPQGFSLVGSSPEVHVKCLGGRIDIRPIAGTRWRGKTRA
                     EDDALAGELLNDPKERAEHIMLVDLARNDVGRVAEYGTVHVSDLMIIERYSHVMHIVS
                     NVDGRLRGDKDAYDVMRATFPAGTVSGSPKVRAMEIINEVEKSKRCAYAGAVGYFGFD
                     GNLDSCIALRTCVLKDGKAHVQAGAGVVADSVPAAEYQETVNKAAGMLRAIEWARQLG
                     A"
     gene            complement(2309..2854)
                     /locus_tag="PLP58_00780"
     CDS             complement(2309..2854)
                     /locus_tag="PLP58_00780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ07084.1"
                     /translation="MRFSISRLRVVLFSPIVVLASCESVPPALDNFIHIKGKPLSMAI
                     RDARAPEPENITASGRPAVYDLVEAGRITGLAEEKSAVHERGRENDKARLTSFRGGVR
                     VGSRVLAVPHASTSAVSSFGFGGGLVVGSVTAVLGDGTVRVRKDHHRPFDEVPEEFAT
                     QRGHLFPPDWSWHRVILHAGD"
     gene            complement(2908..6354)
                     /locus_tag="PLP58_00785"
     CDS             complement(2908..6354)
                     /locus_tag="PLP58_00785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018290830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HRJ07085.1"
                     /translation="MSELKSTVAISLPRDEQNAPANNSGVQTTNTLRDGESPHLISTE
                     RDGYSASHLGIAGRIAATFIDSKLTPLVVIAFVLLGAAAVVLLPREEEPQIKVPMIDV
                     MVAMPGSTAKEIEERATRPMEKLLWEVPGVEYLYSTSRDSESLVIVRFKVGEDPERSL
                     VKITEKLRSNFDRIPMGVTMPIVKPKSIDDVPILALTFHSARYDHLTLRRLAAQVDES
                     IKQVPLVAETTLLGGSRRAVRVMLDPVKLASRNLSPAGLVPMLQQANRQFRAGGLTTG
                     NNEVLVETGAFLKTAEEVGNVVIGVFSGRPVYLREVAEIVDGGEEPTQYVFHGNKTND
                     EPAVTLSIAKRPGANAISVADEVLQKIELLKGSVIPADVSVSITRNYGETAAEKSNEL
                     LLHMGIAVFSVAILIWLTLGWRESGIVAVAIPATLALTLLVFYLYGFTLNRITLFALI
                     FSIGILVDDAIVVVENIVRHFHLPQNKGRKWSAIAVEAVGEVGNPTILATFAVIAAVL
                     PMAFVGGLMGPYMRPIPIGASAAMFWSLLIAFIVTPWASIRILRWGKKYRSDGVMENG
                     SVGNQNSTTPALHYSSAPQHHSSHEHPEDFFTKLYRKMMGPLIHSSGWRWVFLIGITV
                     LLLGSMGTVGIGWVKVKMLPFDNKSEFQVILNMPEGSSLEKTTAVAREMAAAIRTEPE
                     VTDYQVYAGVASPYNFNGLVRHYFMRRGANVADIQVNLVNKHERKAQSHDIAKRVRPA
                     VAAIAERYGARVAIAEVPPGPPVLQTLVAEIYGPDDEARLKLAEKVKAIFKSTPGVVD
                     VDWYIEADQQKARFIIDKEKAALHGISAATISQTLKIAVDGENVDLLHQPEEKEDVNI
                     RLELPRSAKTTPEELLALRVRSGDANALPEPGASGSPLVPLRELVKVEHVTVEKSRYH
                     KNLMPVTYVIGDVAGVVESPVYAIFQMNEALKKLDTREFGGSGAELKILNASMPFSDD
                     EPAMKWDGEWHITIEVFRDLGAAFGACLILIYVLMVGWFRSFITPAIVMIAIPFSLVG
                     ILPAHGMLDAFFTATSMIGFMAGGGIVVRNSIILVDFIELRVREGMPLSEAVIDAGAV
                     RFRPMLLTAMAVVVGAAVILADPIFQGLAIALMAGEIASLFISRMAVPVLYYMANKHR
                     LS"
     gene            complement(6351..7358)
                     /locus_tag="PLP58_00790"
     CDS             complement(6351..7358)
                     /locus_tag="PLP58_00790"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HRJ07086.1"
                     /translation="MKHLSRLFPVLLLAACGKHNHSPAPSATLPSVTVKVEAAKWSPH
                     TAVEEVVGTVRSKQRAVVEAKVSGRVLEYTATPGAMVKTGDLLARLDVQEIQAKVDQA
                     RAMLDQAKRDFDRQKQLIASNATTRQEFDAAEARVKIGTGAVSEAETMLSYAKVTAPF
                     DGVVTRKLADVGDLAMPGKPLLEIEAPTSLRFEADLPEAILDRVKLGAKMPVRLAKVI
                     EGTVSEISPVADPVSRTFNVKLDLPATEGLRTGQFGRVSVPVAEVKLLLVPQNAVLKR
                     GQMELVFVTQNGKAALRLVKTGKVLEGRVEVLSGLEEGEQVIVSETAKLTDGQPVTIQ
                     P"
     gene            complement(7383..7589)
                     /locus_tag="PLP58_00795"
     CDS             complement(7383..7589)
                     /locus_tag="PLP58_00795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018290832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2892 domain-containing protein"
                     /protein_id="HRJ07087.1"
                     /translation="MKLENAIRALAGTMILTSLALAHYVSPNWLWLTTFIGVNLLQSA
                     FTGFCPAETVLKKLGVGGSCCTRS"
     gene            complement(7586..7744)
                     /locus_tag="PLP58_00800"
     CDS             complement(7586..7744)
                     /locus_tag="PLP58_00800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ07088.1"
                     /translation="MTPNRLFILIAALEALLAGMQMTRQGCPSCVLLPKAPEAAPAID
                     LNHTTPKP"
     gene            complement(8021..9427)
                     /locus_tag="PLP58_00805"
     CDS             complement(8021..9427)
                     /locus_tag="PLP58_00805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HRJ07089.1"
                     /translation="MHRLALPPVLALALALHLPSPPAFTAERSLVTDEPVFAKGRPEG
                     AAEYVAKAADTDGQHLALTMHKLQQGLDRPFLIWAIGSSYTNMLGSGEFWQRELPKRF
                     PKTKQVEYRKMVGNSCPWQYLRGWARHLVIPDQPDLIITYTIGDPADLEKLLIELRRH
                     TTADIIVPSIHWRERDIPLWGQSENAADQDVAAVREVCRRHHVEFVENRRDWAAYLTA
                     NKLAVPALLKDAVHQSDYGAHIINSNILAHFRVPQAFSYEPASREKQLQPEKQPDGSF
                     TAKFTGTRIDLHGITAPDGGTFRVLIDEKPARESNAWLMSYVQPDAKNAKEGKGANPR
                     DQSPHGITLGQKIVPQTWTLVMTSDSGDYTLTGSVTGPDGNGNAFKPFTSASGQILIE
                     PDLWRRAERNRTGDRFTFEVKPAVLDEVTFKASLATPFVLRLAQMLSNDDHTLRLVPT
                     KPGTATINALQGFQPPGR"
     gene            9606..9962
                     /locus_tag="PLP58_00810"
     CDS             9606..9962
                     /locus_tag="PLP58_00810"
                     /inference="COORDINATES: protein motif:HMM:NF033788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HRJ07090.1"
                     /translation="MPSTRNKKALPPVTDEALALIASWFRTLAEPSRLKILRALEEGG
                     ELNISELVEATGLTQANVSRHVQSLADAGMVGRRKEGLAVICFIADPSITELCDKVCG
                     NLLKRLSQQVKKLGGA"
     gene            9987..10412
                     /locus_tag="PLP58_00815"
     CDS             9987..10412
                     /locus_tag="PLP58_00815"
                     /inference="COORDINATES: protein motif:HMM:NF012790.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HRJ07091.1"
                     /translation="MIFRRMLWTLVIVVAVIGIWYVYEGSWDRQLFKAAPGRVCANLN
                     ADQANAWLREHPETQVLDVRSTGEFSEGAMPNAVNISIGDEAFDAKVAALAKEKPVLV
                     YCAGGFRSRKAVARLKELGFQNIQHIHRGYMSWKPDTQR"
     gene            complement(10429..11094)
                     /locus_tag="PLP58_00820"
     CDS             complement(10429..11094)
                     /locus_tag="PLP58_00820"
                     /inference="COORDINATES: protein motif:HMM:NF019040.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1501 domain-containing protein"
                     /protein_id="HRJ07092.1"
                     /translation="MPYPPLSLLEARIASYELGYRMQTSAPDTFDLKHEDPATQKLYG
                     LDHPATAEFGSQCLIARRLVEKGVRCIQLRPGGWDAHGSLRSNHTSQSRAVDRPIAGL
                     LTDLKQRGLLEDTLVVWGGEFGRTPTTEGKVTGDKRGRDHSPAGYTMWLAGGGVKGGQ
                     IIGSTDELGFVPVERPIGPNDLHATLLHALGLDQHRVVFGHNNRDEIPTNLGGEVVRE
                     VFV"
     gene            complement(11219..12640)
                     /locus_tag="PLP58_00825"
     CDS             complement(11219..12640)
                     /locus_tag="PLP58_00825"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase"
                     /protein_id="HRJ07093.1"
                     /translation="MRPLLFASLLAAFGTSASGAAQPQRNILFVVTDNQNWFDLGCYG
                     NKVVQTPHMDRLAAEGVRFRQAFATVASCSASRAVIFTGVLTHRNGQYAHTPHEHNQR
                     LRPDITTVFAKLKAQGYRTALIGKQHIAPLDKYPFDFTTAEEKGEVGAYRISSKDVVG
                     LGKACDEFIASCEDQPFFLTLALGDPHPNGITGMNGVAWGVEAKNGYTPIAYDPARIE
                     VPRFLPDTPMVREQLAGYYQQVSRADHGLGLALEALKKHGCTDNTLVIFTSDHGTSEP
                     GAMGTQYEPGVRAPFIVKKPGGPAGIVHEALIAFTDITPTLLDWAGVKDPAKGTHGRS
                     FLPILDQPKAEGWDEVLLSHVAHEIYSYYPMRTLRERRYKLIWNLTWKAEFPLPVDTF
                     SRRLWRHIVETKATHIGPRTVESFLNRPKFELYDLESDSSELRNLATDPAHAERLAAM
                     KARLHQRLIETEDPWLKKYHPDW"
     gene            complement(12666..13976)
                     /locus_tag="PLP58_00830"
     CDS             complement(12666..13976)
                     /locus_tag="PLP58_00830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ07094.1"
                     /translation="MRPLQTVITTFFFALSPASAQTDPASLAWQEKAAGHARILSQVK
                     WSPVADTLPNRKGGYFEKGKEYTGVPYSSVRSEGRYIGFDIALRTFLAAVENPHSVLY
                     TENFTGKVANAAAYYGSVCSAYTSYALGCGIPEVSRRYGPPVGTGIILVEPQSAEAAQ
                     VGDVIYTPHATETSGSHVEMVTAVNRDKECRLVSIRVEESRPPTTATTERSVANFNAH
                     LAARNKQLLRVTDREAWRGANRSEPLLFPNFDLDATPAKINRALLLDLGDWVPYQKGA
                     LVKFHVMDRDQLGVKALVIQRGKDVVEEIALTSPGLHERRFEVCGDYSAHVIHADGSK
                     SQACEFAVCDLDLRLPGESVSLGKEWKVGFGSENMDVIAVYLWNSGDSYGRHPIFLTE
                     AQRKSGALTIPADLLKKPGTLQVWLIGEHALGRLKARKDIPLVK"
     gene            14111..14284
                     /locus_tag="PLP58_00835"
     CDS             14111..14284
                     /locus_tag="PLP58_00835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ07095.1"
                     /translation="MKLLFPTFMLSAMMTLITPPACAGEPKVFRTFMPEAGPSAFGVV
                     LGPELALCYSGPN"
     gene            14263..14541
                     /locus_tag="PLP58_00840"
     CDS             14263..14541
                     /locus_tag="PLP58_00840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ07096.1"
                     /translation="MLQRPELTRLRDTDGDGVADHYETLNDDWQVTDNWHEFTFGLRR
                     DAAGDFILATGLPDVAGEISTRFPREPLRLEKVHEEASLHQAAMRAGF"
     gene            complement(14710..14844)
                     /locus_tag="PLP58_00845"
     CDS             complement(14710..14844)
                     /locus_tag="PLP58_00845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ07097.1"
                     /translation="MNRFQQKLAEHAIPLTRSAFQTLQINAGRKCNQTCTHCYVDARR
                     "
     gene            complement(14854..15777)
                     /locus_tag="PLP58_00850"
     CDS             complement(14854..15777)
                     /locus_tag="PLP58_00850"
                     /inference="COORDINATES: protein motif:HMM:NF025217.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HRJ07098.1"
                     /translation="MGAAGKVIGVDMTDDMLEVARRNAPIVAERTGYANVEFRKGRIQ
                     DLALDLERLDSELKKRPIADTASFLAAHDLAEELRVKHPLIATESVDAVVSNCVLNLV
                     EAREKRRLFEEILRVLKVGGRAVISDIVSDESVPEDKQRDPALWSGCISGALTEEGFL
                     KAFQDAGFHGIEILKRDEQPWQTVEGIEFRSLTVQAWKAGQGPYLDRSQAVIYKGPFL
                     KVQDDDGHAMERGKRYAVCEKTFKLLQNEPYCACFEFIEPRTEVPLDSAPPFDCSSMR
                     LRHPKETKGQDCNATTEAANCRTTGDGTSCC"
     gene            complement(15784..16017)
                     /locus_tag="PLP58_00855"
     CDS             complement(15784..16017)
                     /locus_tag="PLP58_00855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ07099.1"
                     /translation="MTIPTAESATRDRYAAAAKVSEAALCCLASYDPQYLKIIPAEVI
                     EKDYGCGDPSKHLRPGETVLDLGSGTGKICFIA"
     gene            16146..16826
                     /locus_tag="PLP58_00860"
     CDS             16146..16826
                     /locus_tag="PLP58_00860"
                     /inference="COORDINATES: protein motif:HMM:NF025217.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HRJ07100.1"
                     /translation="MKTRLSALFLAFTVFALAQDASVKSGINDKFLDPKLNVEEWTKK
                     FETESREIFHQREKIVAAAGLKPGMAMADIGAGTGLFTLHFAQAVGETGKVYAVEIAK
                     NFLEHIKARASNASASNVQTILCTEKSVELPEASIDLAFICDVYHHFEYPQATLATLH
                     KALKPGGELVLIDFKRIPGQTSDFIMGHVRAGQEVFEAEVTAAGFQKVEETTGLLTEN
                     YFVKFRRK"
     gene            complement(16881..17918)
                     /locus_tag="PLP58_00865"
     CDS             complement(16881..17918)
                     /locus_tag="PLP58_00865"
                     /inference="COORDINATES: protein motif:HMM:NF024676.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="WYL domain-containing protein"
                     /protein_id="HRJ07101.1"
                     /translation="MSRTTTRTVKARKTRTHEARAGASRPAMWRVLEIHKIIRAGRFP
                     NCTTLAREIEVTPKTIQRDINFMRDQLDLPLEYDALRHGYHYTREVNEFPMLRLSRSD
                     LVALFLARHALEPLRGTRLERMLAESFSKIADACPGEVSIQWHELDQAFSVKAAGVLA
                     ADVRLFGDLVEAVRARRVVAFDYHKLTAAAPEGRTVHPRHVGQIEHGWYLIAHDPSRG
                     ALRTFALQRITNLRVLDTTFEPDGSFDARGHLRGGFGVWSYDGAGERHTVRIRFTGWA
                     ARLVAERHWHPSQSIIAVGNENNEIEFQAELPGLEEVTRWVLSHGRHARVLSPNALRV
                     AVQAEARAMLE"
     gene            17964..18680
                     /locus_tag="PLP58_00870"
     CDS             17964..18680
                     /locus_tag="PLP58_00870"
                     /inference="COORDINATES: protein motif:HMM:NF024909.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vWA domain-containing protein"
                     /protein_id="HRJ07102.1"
                     /translation="MSHPLWQGAALMNPHLTEIAYILDRSGSMQNMREAAVAAFNNFV
                     KTQLDVPGDARLTLVQFDDSYEVHVAAKPVQDVPELTAATYQPRGSTALLDAIGRTIK
                     ETDRRLKTLPETEKPGKVIFAIFTDGEENASQDYSAKHISDLIRLFRGEKGWEFIFLA
                     ANQDAIATASAMRMDSSLSSGVAFSHKGIKSTGSAMARKVRAMRMKSSGSMDAQALED
                     DAKSMSEIIQEEENKPDDQA"
     gene            complement(18972..20066)
                     /gene="gap"
                     /locus_tag="PLP58_00875"
     CDS             complement(18972..20066)
                     /gene="gap"
                     /locus_tag="PLP58_00875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007415158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0019682 - glyceraldehyde-3-phosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HRJ07103.1"
                     /translation="MVKIGINGFGRIGRLVFRAICDQGLLGKEIEVVAVNDLVPADNL
                     AYLVKYDSTQGRAKEEVSSKCSPEAIEKARVASEKSGKQVDPEPDILVVDGHEIKCLA
                     VREGPSALPWKDLGVDIVIESTGLFTEKDKAMGHISAGAKKVIISAPGKNEDITIVMG
                     VNHEKYDPASHHVISNASCTTNCLAPVVHVLLKEGFGIEEGLMTTVHSYTATQKTVDG
                     PSKKDWKGGRSAAINIIPSGTGAAKAVGLAIPEVKGKLTGMSFRVPTPTVSVVDLTVK
                     TVKETSYAEISAAMKKASETYLKGILAWTADEVVSTDFIHDQHSSIFDAGSGIELNSK
                     FFKLVSWYDNEWGYSNRVVDLVKFIVGKGL"
     gene            20291..22741
                     /locus_tag="PLP58_00880"
     CDS             20291..22741
                     /locus_tag="PLP58_00880"
                     /inference="COORDINATES: protein motif:HMM:NF019219.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1549 domain-containing protein"
                     /protein_id="HRJ07104.1"
                     /translation="MKISPLPFALTLVLSALAGAHAAPDTASAARQIDSILEADWKKN
                     NLQGNPAADDNSFVRRIYLDTIGRIPTAREAGEFLSSTAPDKRARLIDKLLASDGYTQ
                     HFFNYWSDVLRIQTNGPQAGPVTGAAYADFIKQSLRTNKPYDQMVRDMVAAQGKAWDN
                     GAIGYYMRDRGMPLDNMANTVRVFLGTRIECAQCHNHPFDKWSQMQFYQMAAYTYNVD
                     TQDYYGGSLEDVRELLRERESELRAKFKEPQRPRRDRKMSDAEWARLEKEYRDQAAKV
                     RKEYETARQAMRQEQRNYQEAMTDVRNTMRYTAVDMRNRNLTLPHDYQYSDAKPRSTV
                     QASVMMGHECATQPGETPLQAYARWMTSKDNPRFTSVIASRLWKKAFGLALIEPLDEL
                     MDTSTPMIPELQTHLEGLMKSLDYDMKAYLRVVFNTSAYQRQVTREEVPPGVAYHFTG
                     PLLRRMTAEQMWDSFVTLINPNPDMPNLRLREDAEQRILQAKKNADGVDALSVEEALR
                     GIKLSAAVYDKNRERTEAAQKLYLEARIRHKELQDEADSLKAGPERDALLVKVADAKK
                     KSDDLRRQVNDIQNEGRRTSTQEIIVAGHKKLYEVTTGKPWQPVSKAVKDSTDGSEPA
                     MMASDTMMMAYGVRAERVTIPGYDRPELSKDERKAREDAMREEFSEEARFYGLPEKEL
                     RDYFRSRETQNRTYVRAAEEQSPAPRGHPLRDFGQSDRETIENANYDASVPQSLFMMN
                     GSLLPNILHRHSQLMLTINKAQYPDDKVEAAYMALLARKPTSKEMETWNKAADAGLDK
                     IEDLVYALLNTQQFIFIQ"
     gene            22787..24100
                     /locus_tag="PLP58_00885"
     CDS             22787..24100
                     /locus_tag="PLP58_00885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009961823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1501 domain-containing protein"
                     /protein_id="HRJ07105.1"
                     /translation="MKQELASKLLRSGEITRRDFAAKTASSLLGVGLLGHSLSSRSYA
                     AFEQSSKLRQTATAKNVIYLYMSGGQTHLDTWDPKPGTETGGPTKAIKTSANGVQLAE
                     NLPLTAQHMHHGTVFRSLSSTQGAHEQGNYMMHTSYELRGTIRHPGMGAWLNVFQGGG
                     NSTLPKYVYVGNDSRHPGAGFFPAAQSPLFVNNPEAGLKNVKPHSGVTEEKFNARLKL
                     AGELDADFRSTFPHRNVKAYAEMYDDAIAMMRSEDLKAFDLSEEADDIRAAYGRQSFG
                     QGCLLARRLVERGVRFVEVSLGGWDTHNANFVRVPELCETLDRGLSALLADLQNRGLL
                     QETLVVVATEFGRTPDINQNVGRDHYPKAFSAAMFGGGIKGGNVHGQTDPEGREVVAD
                     KVKIQDFNATIAYALGLPLDQVIYSPSKRPFTIADKGHPVTGVFA"
     gene            24254..26857
                     /locus_tag="PLP58_00890"
     CDS             24254..26857
                     /locus_tag="PLP58_00890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018290296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015616 - DNA translocase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HRJ07106.1"
                     /translation="MPDAEIIKPRPMRARVQEKENPWNDATGITFLVLATMYLLALIS
                     YDPGDLPAWCHLSPADEPNAVTQNFIGTAGALAAGYSLFLAGLSAYILPFSMTWFGVC
                     KLASHVRITGRSWFGVLLMILSISALLEVQNIMNERDEITPLGGGGGIGYLIGGSFLA
                     GLLGTAGSTIVLSGIYGVGFFLASGLHPLLVLQDIRREIQEFKERWRVRRELAARQAA
                     EPAPAAGTGIIRRKRPAKSETADETPPSGPMVTPELGLTFEPQRPKIFDANEVRHPKD
                     AADKPKLSEVWEKKRAQKLEQAPHGTLGSLAVLFKDYKLPDTDLLHWPDETQRVTADK
                     GELLAVQNTIIRCLASFNVKVEPGDITRGPAITRYEVRPEDGLRVARIAALDNDIARA
                     TCAERINVLAPIPGKDTVGIELANREKIIVPIRELLEDEIFSNGKARLPIALGKDVYG
                     KTIIGDLAAMPHLLVAGATGSGKSVCINGIITSLLCRFAPDELRFIMIDPKVVEMQNY
                     ADLPHLAVPVVTDPKKALLALRWVVKEMENRYQIFAQEGCRNFDAFNTRNRKRAAASQ
                     ARKAAAAKNQPPISAGTDDARASAAFAKVLEPDTAGSAATADGSTPENETEILPVDAS
                     GMWTGDSAPPPPRRHEVVIPDTLPYIVCIIDELADLMQTAPADIEVAIARITQMARAA
                     GIHLIVATQTPRADVITGVIKANIPTRIAFQVSSALDSRVILDRKGAENLVGKGDMLY
                     VPPGGAQPIRAQGALVTDDEIHAVVEHCVTQGKPVFDIKVDDETGEMDGEEFEDSGGG
                     VSGEEEETLEKCLEVIRQEKKASTSLFQRRLKLGYGRAARMMDILEDRGIIGPGEGAK
                     PREILVDMDMI"
     gene            26969..27886
                     /locus_tag="PLP58_00895"
     CDS             26969..27886
                     /locus_tag="PLP58_00895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-rich repeat domain-containing protein"
                     /protein_id="HRJ07107.1"
                     /translation="MTQLPRLLPALLILTACGDKNPPVSGAQPAAPAASPAKPAVTSQ
                     PPSAGAPASPKRIWWTAEMLHTEIKYHNPDYAGGGEFAIEQGEVLALNLRESQVTNLV
                     FLERLSPLALDLSATPIKDIRPLKNMRLQELYLEDSPVTDLSPLRGMPLQKLYLSRTP
                     VSDLSALAGAPLVELNAVETRVSDLSPLAGCPIQMLWLTGTPVEDISPLKNVPMISLT
                     LHRSRVRDLGPLAGTSLRRLHIGETPVTDLTPLKGLPLTRLVFTPASIKSGIEIAKAL
                     PLDEIGTRFDEERDLVPPAVFWPRFENGQ"
     gene            28025..28810
                     /locus_tag="PLP58_00900"
                     /pseudo
     CDS             28025..28810
                     /locus_tag="PLP58_00900"
                     /inference="COORDINATES: protein motif:HMM:NF015912.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 factor domain-containing protein"
     gene            29348..30061
                     /gene="rpoD"
                     /locus_tag="PLP58_00905"
     CDS             29348..30061
                     /gene="rpoD"
                     /locus_tag="PLP58_00905"
                     /inference="COORDINATES: protein motif:HMM:TIGR02393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HRJ07108.1"
                     /translation="MIEANLRLVISIAKKYTNRGLSFLDLIQEGNMGLMRAVEKFEYR
                     RGYKFSTYATWWIRQAITRSISDQARTIRIPVHMIETINKLMRVHKQLVQELGRDPTP
                     EEIAEEIHLPVERVNTVLRMAQQPVSMQTKVGDVEGDTEFGDFIEDKDASDPMELTAK
                     NLLRDKLKDVLDTLNPREREVLEQRFGLMDGSGRTLEEVGRQFQVTRERIRQIEAKAL
                     RKLRHPTRISKLGGFYGGK"
     gene            30116..30268
                     /locus_tag="PLP58_00910"
     CDS             30116..30268
                     /locus_tag="PLP58_00910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ07109.1"
                     /translation="MNTDPQPDPQEQTAVKEQNDTLVEDDFAGEVLGERQAEACNLEE
                     GCERCQ"
     gene            30391..31824
                     /locus_tag="PLP58_00915"
     CDS             30391..31824
                     /locus_tag="PLP58_00915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009959889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HRJ07110.1"
                     /translation="MSAIPHLPALRKGRPYESLDKVQVKDHKTGEVCAEVSQVNAGIV
                     RKDLGKISEARKALKKFTVAQLIEITAKAGESFLNGTLPLGDKGHTQSPEEYIATLSR
                     TSGLPHIMVKRNMAKIHYAMTHMSVILNGLSRGLDMSVIDRGFGEQSGCPVAYFPTTH
                     SLGLVMPSNSPAVNSLWLPSIALKIPVVIKPGREEPWTPFRLIQAFIAAGAPPEAFGF
                     YPTDHEGSGEVVKLCGRNLVFGDVNIAKMYENNPRVQVHGPGWSKIIIGADKIENWRE
                     YLDVIVGSISDNGGRSCINASAVIVPKYGKEIADAIAQRLGPVEPLPADDENARLSGF
                     ANPKMADYIDAAIDNDLKIPGAEDATAKYRNGPRKVEFQGGIYLRPTIIRCDSWEHPL
                     ASREFLCPYASVVEVPQNEVLSKIGYSLIVTAITQDEAFINDLLECGDIDRLNIGPIS
                     TMKISWDQPHEGNMFEFLYKRRSIDCA"
     gene            31893..33014
                     /locus_tag="PLP58_00920"
     CDS             31893..33014
                     /locus_tag="PLP58_00920"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009959890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HRJ07111.1"
                     /translation="MIVPSPFDYQPRTRLVFGNGTLSRVGELAGELRGTRALVVSDPG
                     IVGAGYVTRAVSLLVEAGLEARVYGDVRENPSTEDVDRCVEAAREFQTDLIIGLGGGS
                     SMDTAKGCNFILTNGGRMQDYWGTGKASKPMLPLIAIPTTAGTGSECQSYALISDAET
                     HVKMACGDPKAAAKIAILDPELTVSQPARVTVCTGIDALSHALETAVTSKRSALSSVF
                     SREGFRLCHEGFSRVLRDPADIEARGMMLLGAAYAGTAIENSMLGAAHSCANPLTAYF
                     DVVHGEAVGVMLPHVIRFNSALPEIAAIYAGYFDGDLADRVTELLWEAKMPRKISDYG
                     ATEADLPKLASLAAKQWTAQFNPRPVAEADFVELYRAAL"
     gene            complement(33015..33593)
                     /gene="rsmD"
                     /locus_tag="PLP58_00925"
     CDS             complement(33015..33593)
                     /gene="rsmD"
                     /locus_tag="PLP58_00925"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: protein motif:HMM:TIGR00095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052913 - 16S rRNA
                     (guanine(966)-N(2))-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HRJ07112.1"
                     /translation="MRLISGSAGGIPLDVPKSVTRPTQDRVRQAVFNMLGPLVEGARV
                     LDLFAGSGALGLECLSRGSESALLVDQDRGACEVIRRNLAKTRLAGATVRQGDVFKTV
                     GQLARESACFDLVFADPPYAHKPGEADLCAKLLESADFTALAPAGASVVLESLATRRS
                     APPAPEGWELMRERDYGSTRIVWLRRVGSGHG"
     gene            complement(33727..35103)
                     /locus_tag="PLP58_00930"
     CDS             complement(33727..35103)
                     /locus_tag="PLP58_00930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009963897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-dependent sugar dehydrogenase"
                     /protein_id="HRJ07113.1"
                     /translation="MKTLSSLSVLAALLGASSTASAAQPKPAQPGGKLPGNPAIALVK
                     VADDLVDPVSVAAPDDGTGRVFIIERPGVIQVLKDGVKQRRPFLDIKDKTISSFLELG
                     MYSMAFHPKFKDNGKVYVAYADLWFNGATLITEYTVSKSNADRLDPDSARVIMHIDFP
                     YCNHHGGQIAFGPDGYLYIGAGDGGWEGDVINAGQDLHTWLGKMLRIDVDHSTPDRAY
                     SIPKDNPFITPLQQMTLFGVSEEAFSKIHPKAKPEIWSYGLRNPWTFSFDSKSGDLYI
                     ADIGQNHWEEINMQPASSKGGENYGWKFMCGSHPFPMILKKNPDGSETAVDPENYPEV
                     GVLPIGEYSHVDQGICVINLGVYRGKDYPELDGIYFCADWGSGKIWGMKQVDGAWQMQ
                     ELLDLADGIRPTGSGTGPDGAIYMSHATANYGGPVDPYTSERGAVWKIVPAGKVPAGA
                     VTAPVSAK"
     gene            complement(35285..36976)
                     /locus_tag="PLP58_00935"
     CDS             complement(35285..36976)
                     /locus_tag="PLP58_00935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ07114.1"
                     /translation="MKNPILHIVQSVLVLGFMATMGDIRAQDVFTPFAGSYQGLLVDS
                     TSGDPAGRVEIALTSKGALSTTFTMLNQKTYKAAGKAAFDEVNDWAELENFNVVKPKA
                     GPPPLSFVINLYLKKDGTFELTGAAELPGYTGSFTVEAGTASKLRFYKAKTDDCPWMG
                     TYTLAFPDADDLGSTPPPGGVCIGSAVIKPDGVLALKGTLADGTKITASARPSQDGIY
                     RFHILVHKTIGSYFAFWFQLTARGDGWFHSAEGDGWARWSKAENQKDKTYRDGFDVEF
                     ETRVTQWKPPGKGETLQGILGMGDDQVLDIAFFNGLNTTTYAKFLPSQLGITAKNIFR
                     VAAGLSGSPSPLYPEQWAKVFSGKIDPKTGLMTLVLNIQDTVTTGTLPKLTTKTIKRK
                     VVINGVYQQLMANDLLVPYAYGHLLIPPLDPKTQTLISGGFDLPGPVELDPFVASAGQ
                     TAGIYSAKLTEQPHPSPPPSGLPPVAPASVTFSISSNLKEMIFNGRKLPLKGDSRPVS
                     LVYTDADKSAGNNVSVTVYLNGAGVVNSLATQYFQLSGFTVKVRNHTSNAVNKQP"
     gene            complement(37034..38896)
                     /gene="mutL"
                     /locus_tag="PLP58_00940"
     CDS             complement(37034..38896)
                     /gene="mutL"
                     /locus_tag="PLP58_00940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009962921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HRJ07115.1"
                     /translation="MSKIRILPDALASQVAAGEVVERPAAVVRELVENSLDAGAGHIT
                     VEVRRGGSALIRVTDDGGGMDREDAMLCMERHATSKIRTKEDLAAVGTFGFRGEALPS
                     IASVSRFRLATREREALAGTEIEINGGRLAAVKDHGGAHGTVIEVRSLFFNVPARRKF
                     LRTEATEYAHVEQQFRLHAIAHPETAFTLIRDDEVAFHLPATRDLLERIRGLVGEDLA
                     SRLIEVEPVEHHGIRIEGHIGGPGLSRSNRQQQLTFLNGRPIESAIINYGLKEGYHTA
                     LMKGQHPVTFLFLRMPPETFDINVHPAKKEARFHDGYAVREAVARAVGRALESAGRLP
                     GGHSTLSPRPQAFAPAQVQTALPLQTARVATGDAPRVVQAEPARPPMTSRMFTGPDAN
                     SAEKIIAPSPVVTTPEAPPPVPNPEAAVEKKARGVPAFRIIGVLHKLYILMESAEGLV
                     LMDQHAAHERVNFEKMRRAMEQGGVPSQRLLMPLTLQLSPRDADVLARNLEPLSRLGI
                     EAEPFGPNIFKIEALPSFVRADDPAAWLDRVVEELKSLSSRTSSLRLGEDMIATTVCR
                     HSVKANDRLSLPEQQALLEDLFACEMPYCCPHGRPTLIQMSLGELERKFGRQAP"
     gene            38989..39324
                     /locus_tag="PLP58_00945"
     CDS             38989..39324
                     /locus_tag="PLP58_00945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009962919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly accessory protein"
                     /protein_id="HRJ07116.1"
                     /translation="MIQLTDSALQHLRHLLDEKGAPVGSGLRIAVERGGCAGLQYTMR
                     ISQPQDGDEVDSKQGVPLIVDRESLAYLEGCQIDYLESLHDSGFKIENPNAARSCGCG
                     TSFEPLKGS"
     gene            39453..40253
                     /locus_tag="PLP58_00950"
     CDS             39453..40253
                     /locus_tag="PLP58_00950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ07117.1"
                     /translation="MQVKFLSDGSEYRSYGGGSTTQKKEGGGIFWWAVLITLLMGAAT
                     FCWFFSIMVFSQPEKPFNYKILAKFKKVTPLRPYTLYTAPAGKAFGPRELLTEFYPYS
                     LEQLQLRNDLLKRTYLKNYQHEQPVYLLGSYKVLEVRQLTEGDVFSSGWVLHARASEF
                     EDVDMEFVMPGLSLADAPCAAGDVIKLDKRAYASVLHLERLGQDRVCATVVPLAYQTV
                     VFANGKSHSLTPPELLNIESVWPLTRDPGAESIKPVDGEKAGIAATQP"
     gene            40358..40771
                     /locus_tag="PLP58_00955"
     CDS             40358..40771
                     /locus_tag="PLP58_00955"
                     /inference="COORDINATES: protein motif:HMM:NF024245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HRJ07118.1"
                     /translation="MAPDPNEPAFPSSAPYSPEAKTDPREEAVSSSMLIWVFIWCVGA
                     LVSQDMAGKSNSAQNTDTADIAPSRQSTALVDLNSATPAELERLPGIGPVLAIRIVEH
                     RPYRSIEELARVPGIGEKIILQLKPLVKTEVSTPD"
     gene            complement(40780..41697)
                     /locus_tag="PLP58_00960"
     CDS             complement(40780..41697)
                     /locus_tag="PLP58_00960"
                     /inference="COORDINATES: protein motif:HMM:NF015431.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HRJ07119.1"
                     /translation="MFRELASLSGLSLERLASLCEVGEAGSIGQAARGDANRQSLLSR
                     QIAELEAVFGVALLARQSKPFRLTEEGRELARAARTFFQAAEDVRSRAGGEARRVVIG
                     AGETLIQWLLFPACEAVRRGLGEPVVFAFKNLESARLVEVLHQGVVDAALIRPEDAGP
                     GLVCARAWRYEQAAFVPKALSGGTAALRAVDLGAFSWAVLEGRGHFRRFLEERARAAG
                     VRLNAALECSSYTQVALAVRSGRYAGFLPEFAKPYLGGGEPVIRRALESALAYQRGVV
                     LAWREAGVKARPFLAEVFEGLERGLGRRL"
     gene            41843..43825
                     /locus_tag="PLP58_00965"
     CDS             41843..43825
                     /locus_tag="PLP58_00965"
                     /inference="COORDINATES: protein motif:HMM:NF024573.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HRJ07120.1"
                     /translation="MISQLTLENFKAFGNAQAVPVRPLTLIFGPNSGGKSSVLHSLKW
                     AKHAVKSKSLTFESPGGRDDAPDFGGFLGAVFGKDRHRRIRFGFLLPSRNAPDYRVSF
                     ELGLWTRELKRLVRKEPEPPNEIKDWVAAQNEFIDAETEASLRKEYLHNFVFDEDGNP
                     VRDDNGELIDLKHDGSERIDFEALAEKIRQYWDPRLEELWNTWRSENAKPGISEKVGR
                     YCERLEHYHDWLDKQETDPVVFGFEIAIGENVLLKAERKTFDGFVQLTKVSRALSAVW
                     SYMDEGSDSAAWEASSWSTPPPWVVTHPSQVLDLADLWDEGIWSGEDLGDCLGIEFQG
                     LIPSLANQAVFSGQCDLAMEKQDLECLEKLIRDCRVRLEGTMTKMSYLGPLRTLPPRS
                     LLLFEKDERSGAVADAWLRLRDDSKLRDEVNRWIGSGHLDLGYRYESKVYCDTYSLLE
                     TMRNEERALSIHREWNLADESYESGYKVKDLHESKTPDFSDNEYEAVNFWNDRISEKL
                     HELVLRDLKNNVAVSNRDVGVGISQLIPVIVHALAEKGAAIAVEQPELHLHPALQAKL
                     GDLFIESALGRGNTFVLETHSEHLILRLLRRIRETTEGELPDGKFALTPDQICVLYVE
                     PGEQGSKILELPVTPDGDFSRPWPQGFFEERSAELF"
     gene            43834..44772
                     /locus_tag="PLP58_00970"
     CDS             43834..44772
                     /locus_tag="PLP58_00970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ07121.1"
                     /translation="MIKEIALDPCLFAQWQHYLALRDVFGVEKGRVISAFSKKWKGLV
                     REEVNRLIAAELIGANKGKSILAWLTVPFGTTETRMVTSAAAYDGNKPWHLNAEDNWS
                     SFDAILSHREVTSENAVLADDEYEYLRHPKLTPETQRTIARQRGPIVDCAWSLLRDSK
                     VVKLVEPHFNPHKPRFLDVLEAMLDRLHTEGSSVREIELHVRHPEDKDPQGTTPPLFT
                     VPELRSKLSPLLRSGWSLRVHLWRRGREKMHPRYLLTDRGGIQIDHGWDEGEHDTETT
                     PILLLNRARWEQEMQRYQPCSQDFMMNPVTDIVHIT"
     gene            complement(44785..46737)
                     /locus_tag="PLP58_00975"
     CDS             complement(44785..46737)
                     /locus_tag="PLP58_00975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009965928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecQ"
                     /protein_id="HRJ07122.1"
                     /translation="MRVDIHETLRCRFGLAGFRPGQERVMRALLEGRSALALFPTGAG
                     KSLCYQLPALHLEGVTLVISPLIALMKDQVESLQRRGIPAARLDSSLSADEARQVYDD
                     LQSGALKLLYIAPERLMNENFVVRLKRARIAMMAVDEAHCISEWGHNFRPEYLRLALM
                     ARELGIHPVLALTATATPAVARDICSAFAIAGADHVQTSFLRPNLQYHITPSAGRQRL
                     DTLTARLRQRGGPAVVYVTLQQTAEHVATHLQKSGLNARAYHAGLADEHRHEAQDAFM
                     SGAVDIIVATIAFGMGIDKAGIRAVYHYNLPKTLENYMQETGRAGRDGQPSICELLAC
                     ADDRIVLENFTFGDTPTPQALRQLLDHLLRQGEEFDFSLYELSHATDIRPLVIETVLT
                     NLELGGILRPLGSFYASYQYRLLQPLERVLAGHKPARQDFLRRLFATGKKARVWTTVN
                     PDEAALELQEPRERILSALTWLQEAGEIELRPSGSRQKYRLCPDAARRDPAALARQME
                     QLFTRREAREVERLAQVLDYASHRHCLVRWLLRYFGEELPSDCGQCTSCQEKRTGDAD
                     LESPRHIPAAAAPEITTEDVALIRALLDERHAALRTPRQLARFLCGLTSPATTRARLT
                     RHDSFGLLDRVPFAEVLAQAESMTGG"
     gene            complement(46750..48681)
                     /locus_tag="PLP58_00980"
     CDS             complement(46750..48681)
                     /locus_tag="PLP58_00980"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: protein motif:HMM:NF002730.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003952 - NAD+ synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0004359 - glutaminase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) synthase"
                     /protein_id="HRJ07123.1"
                     /translation="MSDPTQNLPEHEKHGFVRAAVVSPELRVGDAEFNAGVIRAEMKR
                     LSGEGCRVAVFPELCLTGYSCADLFYLPTLQKQALAALRQLAEESSYSGMLTVLGLPL
                     AVDGRLYNMAAVAGAGRVLGFVPKTFLPNSGEFYERRWFSPAHTLGVAEVEGVPAGAD
                     LLFEARDLPGLVLGVEICEDLWSVIPPSSHAALAGATVLANPSASNELLGKAPYRRNL
                     VLQQSARCLAAYLYASAGAGESSTDTVYSGHGIIAENGALLAESERFSFETRAVLADV
                     DLQRLEHERQRSAAFRDEAAGRCFRRVVCTLDGRRAASSRLLRPLSAQPFVPPPGSDR
                     DAACREIFAIQSTGLARRLRQTKSAAAVIGLSGGLDSTLALLVLLDALPRAGMSRECA
                     LAVTMPGPGTTERTRGNAEKLAGALGVPLRVIPIHAAVERHFKDIGHPPDRHDITFEN
                     AQARERTQVLMDVANQTGGLVVGTGDLSEAALGWCTFNGDHMSMYHVNAGVPKTLVRY
                     LIEWCAAGQYAAEAGEILRDIIATPISPELLPAAADGGIGQRTEDVVGPYELHDFFLY
                     HFARHGCGREKLRYLAGIAFAGSHTPEVIEKWLGVFLRRFVQNQFKRSSMPDGPKVGT
                     VALSPRADWRMPSDYGGSL"
     gene            48865..50523
                     /locus_tag="PLP58_00985"
     CDS             48865..50523
                     /locus_tag="PLP58_00985"
                     /inference="COORDINATES: protein motif:HMM:TIGR00704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005436 - sodium:phosphate symporter
                     activity [Evidence IEA];
                     GO_process: GO:0044341 - sodium-dependent phosphate
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na/Pi cotransporter family protein"
                     /protein_id="HRJ07124.1"
                     /translation="MILTFLTILGSLGLFLFGMKIMSEALQKLSGEKLRGMMRVMTTN
                     RFTGLLSGFLVTCLVQSSSATTVMIVSFVNAGLLTLFESIGMIMGANLGTTTTFWIIS
                     FLGFKFSLSSIALPIIGVGFPMMFMRNSRVRDTGEFLLGFGLLFFGLLMLKDSVPNIQ
                     DNPEFLKFVQGFTGKGIFSVLFFFAFGTILTIIVQSSSVAGAITLTMAYKGWIDFPSS
                     AAIILGENVGTTITANIAALTGNITAKRAAFAHFIFNVIGVIWAILLFAPFTAFISAI
                     VPGDAGQPEAMPLHLAAFHSGFNLINILLMIGLVPLLVRLVEKTIRSRAPGVASHVEY
                     KRASLPQTAELNLAEAEREVQSLATHTRTLVSGFLDVFGRPEAEISGRITELRQVEEL
                     SDEIALDVTQYLVRCSSAGLSDSSMGRVSVLLRVIAELEDMCDCGNRLVQLASRRTRK
                     GHELPPETLGQLRDMGGHLFTFIDFLSTCLTRPGQPGDMETAQEMERQINACRKRLRK
                     ESMTRMLASGETIKAEVLYIDVLNNMQRISKHAMNILQALRQEG"
     gene            complement(50560..>52096)
                     /locus_tag="PLP58_00990"
     CDS             complement(50560..>52096)
                     /locus_tag="PLP58_00990"
                     /inference="COORDINATES: protein motif:HMM:NF013069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HRJ07125.1"
                     /translation="AYPLPAFAVVAGFAALTLWLLRALPSEFTPKEDRGSFFIVSSAP
                     QGASYGYSMQIFAELERRLMYLVDSGEAQRLLVRIPRSFTSNYDFNEVITIVNLGDHG
                     KRRSAWVVMDEVRAKLADMRGVQNFIIMRQGLSRGLTKPLQFVIGGPTYEDLARWRDI
                     ILEKAAANPGLVGLDYDYKETKPQLEVAIDRVRAADLGVSIANIGRTLESLLGFRRVT
                     TYVDAGEEYDVILEGDYEGKRLPGDLQGIYVRSETSGRLIPLANLVSLREFADSGALN
                     RYNRMRAITFDAGLAPGYSLSEAVAFMESLVRENLPADATIGYKGDALKLRETSGSVL
                     FVFGLSILVVFLVLAAQFESFVHPFTIMLTVPIAVGGALIGLLVMGQNQSLYSQVGMI
                     MLVGLAAKNGILIVEFINQLRDEGMAYDDAVIEASSTRLRPVLMTTLTTVMGSVPLFL
                     GGGPGAETRLVVGVVIMFGVAVSAVLTLIVVPLAYRAISRRTGSPGDMSRKVDAALAA
                     AGE"
BASE COUNT        10121 a        16721 c        15795 g         9459 t
ORIGIN      
        1 catcgcagac ttcgccgccg acctgcgcgc ccccacgccg agcgccgccg ccgagctgct
       61 cgcgccggaa gcggccggac tgctgcgcca ttttgaaacc cgggagcgcg gcatggagaa
      121 ccgcgtcgaa aacctccttg agcatcacga cagggtgctc gatctcaccg ccaggggcgc
      181 gctcgcgcgc ggcccggagt ggcagcttct gcagtccgtg cagcaggcgg atgaagccga
      241 gtcgcgactg aacgcggggg cgcgcgcgca aatccaggag ctggcagacc agaccgcgcg
      301 ctgccagcac accctcgcac gccggcatcc cgagattttg ctttccgagg caggccacaa
      361 ggtcgaaacc gccggacgcg gcatgaatca tctggcggcg cggttgctgg aacgaatcca
      421 gcagcgcctc gactcccgcc gggacctcct gcgccagctc ggcccgcacg ccgtgctcgc
      481 gcgcggcttt tcctacacca cggactcctc gggccgcgtg ctcaaaaatg ccgccgatgc
      541 cggcgatggc gaactcatcc gcacactgct ggccgaaggc tgcattgaga gcgtcgttcg
      601 caaaaagtga ccggagggcg gatgcgtagt cacacaagca gccgcgcatg cgggcaggct
      661 tgatttgcag cgcatcccct tcccctccca cttttgggcg ggcaccggaa cgcgggtccc
      721 gcaggccatg cccataagcc ttgccgcgcc gggatttcgc atccatcatg cgcgcctttt
      781 cccgcatgtc cgctccgcat ttgcagccag accccgactc ctttcgcgcc ctcgcgcgga
      841 aaggaaacct cgttcctgtg gtcgccgagc tgacagcgga ctatgagact ccgctctccg
      901 cctttcaaaa gctccacgac ggacgctgcg gattcctgct ggagtccgcg gagctgaccc
      961 agcactccgg gcgctactcc ctgcttggca gctccccgcg catgatcttc accgcgcgcg
     1021 gacgggaaat cgaaatcgag gagcgcggca ggacccgcgc cttcaccacg gattccgacc
     1081 ctctggacga gctgaaaaag ctcatgcgcc cctaccggcc cgtggagtcg caccccatcc
     1141 ccgtcttcca cggcggcgca gtcggctacc tggcctatga catggttcgt tttttcgaac
     1201 ccaccctggc gccgccgcca aaggacgatc ttggcctgcc tgacatggtc ttcatgatca
     1261 cggacacggt cgtcattttt gaccaccgca cgcgcaggat gatgctcgtg gccaatgtcc
     1321 acaccgacga gcatgagaac ctggacgccg cctacgactg ggcgcgggcg cagatttccc
     1381 tgctcattca aaagctctcg cagcccctgg cggtgaagcc gctgcagacc atcgccccca
     1441 tctccaacga cagcctcccg cctcccgccg gcaacaccac gcgcgaggaa tacgagggca
     1501 tggtggtcaa aatgaaggag tacatccgcg ccggggacat tttccagggc gtgccctccc
     1561 agcgcttcga gaccgcctac actggcacgc cgctggacct ttaccgcgcg ctgcgccatg
     1621 tgaacccctc gccctacatg ttctgcctgc aattccccca gggcttcagc cttgtcggca
     1681 gctcgcccga ggtgcatgtg aaatgcctgg gcggccgcat tgacatccgt cccatcgccg
     1741 gcacgcgctg gcgcggcaaa acccgcgccg aggacgacgc gctggccggc gaactgctca
     1801 acgacccaaa ggagcgcgcc gagcacatca tgctcgtgga cctggcccgc aacgatgtgg
     1861 gacgcgtggc cgagtatggc accgtgcacg tcagcgacct aatgatcatc gagcgctaca
     1921 gccacgtcat gcacatcgtc agcaatgtgg acggccgcct tcgcggagac aaggacgcct
     1981 acgacgtgat gcgcgccacc ttcccagccg gaaccgtcag cggcagccca aaagtgcggg
     2041 ccatggagat catcaacgag gtggagaaaa gcaagcgctg cgcctatgcc ggcgccgtcg
     2101 gctacttcgg cttcgacggc aatctcgact cgtgcatcgc cctgcgcacc tgcgtgctca
     2161 aggacggcaa ggcccatgtg caggccggcg ccggtgtcgt ggccgactcc gtccccgccg
     2221 ccgaatacca ggagactgtg aacaaagccg ccggcatgct gcgcgccatc gagtgggcgc
     2281 gccagctcgg cgcgtgaggc aaaaggcgtc aatcaccggc gtgcagaatg acccggtgcc
     2341 atgaccagtc gggcgggaac aaatggccgc gctgtgtggc gaactcctcc ggcacctcgt
     2401 caaacgggcg gtgatgatcc ttgcggacgc gcaccgtgcc gtcgccgaga acagcggtca
     2461 cacttcccac caccaaacct cccccaaagc caaaggagga aacagcggaa gtgctcgcgt
     2521 gcgggaccgc cagcacacga ctgccaaccc tgaccccgcc gcggaaactc gtcagccgcg
     2581 ccttgtcatt ctcccttccc ctctcatgca cggcactctt ttcctcggcc aggccggtga
     2641 tcctgccagc ctccacaagg tcataaacgg cgggcctgcc ggaagccgtg atgttttcag
     2701 gctccggcgc cctcgcgtcc cggatcgcca tgctgagcgg cttgcctttg atgtggatga
     2761 aattgtccag cgccggcggc acgctctcgc acgaggcaag aacgacgatc ggggaaaaga
     2821 gaacaacacg aagccgggaa atcgaaaatc gcatggcggg gaaaagtgtc gcccatgttg
     2881 ctctcgactt gcggttaaag tcaacggtca agacaggcga tgcttgttcg ccatgtagta
     2941 caacaccggc acggccatgc ggctgatgaa caggcttgcg atttcgcccg ccatgagggc
     3001 gatggcgagg ccttggaaga tcgggtcggc gaggatcacg gcagctccga cgacgacggc
     3061 catcgcggtg agcagcatcg ggcggaagcg cacggctccg gcgtcgatca cggcctcgct
     3121 cagcggcatg ccttcacgca cgcgcagctc gatgaaatcg acgaggatga tcgaatttcg
     3181 caccacgatg ccaccgccag ccatgaagcc gatcatgctc gtggcggtga aaaaggcatc
     3241 cagcatccca tgcgcaggca aaatgcccac cagcgagaat ggaatggcga tcatgacaat
     3301 cgccggcgtg atgaaactcc ggaaccagcc gaccatgagc acatagatga ggatcaaaca
     3361 ggcaccgaag gccgcgccga gatcgcggaa gacctcgatg gtgatgtgcc attcgccgtc
     3421 ccacttcatc gcgggctcgt catcgctgaa gggcatggag gcgttgagga tcttcaattc
     3481 cgcgccgctg ccgccgaatt cgcgcgtgtc gagcttcttg agcgcttcgt tcatctggaa
     3541 aatggcatac acggggcttt cgacgacgcc ggccacatcg ccgatgacgt aggtcacggg
     3601 catgaggttc ttgtggtagc ggcttttttc gacggtgacg tgctccacct tcaccaactc
     3661 gcgcaaaggc acgagcggcg agccgctggc accaggttcg ggcagtgcat tggcatcgcc
     3721 ggaacgcaca cgaagagcta aaagctcctc cggcgtggtt ttggcactgc ggggcagttc
     3781 gagacgaatg ttgacgtctt ccttctcttc cggctgatgc agcaaatcga cgttttcacc
     3841 atccacggcg attttgagcg tctgtgagat cgtggcggcg ctgatgccgt gtagcgcggc
     3901 tttttccttg tcgatgatga agcgggcttt ttgctgatcg gcctcgatat accagtccac
     3961 atcaacgacg cccggtgtgc tcttgaagat ggctttcacc ttctcggcca gtttgagccg
     4021 cgcttcgtca tccgggccgt aaatctccgc gacgagcgtt tgcagcaccg gcggtccagg
     4081 cggcacctcg gcgatggcca cacgagctcc ataacgctcc gcaattgccg ccacggccgg
     4141 tcgcacgcgt ttggcgatgt cgtggctctg cgccttgcgc tcgtgcttgt tcaccagatt
     4201 gacctgaatg tccgccacgt tcgcgccgcg acgcatgaag tagtgtcgca ccagtccgtt
     4261 gaagttgtaa ggcgacgcca caccggcata cacctgataa tccgtgacct caggctccgt
     4321 gcggatcgcg gctgccatct cgcgagcgac ggcggtcgtt ttttccaagg aagagccttc
     4381 cggcatgttc aggatgacct gaaactcgct cttgttgtcg aagggcagca tcttcacctt
     4441 cacccagccg atgccgacgg tgcccatgct gccgagcagc agcacggtga tgccaatgag
     4501 gaaaacccag cgccagccgc tgctgtggat gagcggtccc atcatcttgc gatagagctt
     4561 cgtgaagaag tcctccgggt gctcgtggct ggagtggtgc tgcggagcgc tggagtagtg
     4621 gagtgctgga gtagtggagt tttgattccc aacactcccg ttctccatca ctccatcact
     4681 ccggtatttc ttcccccaac gcagaatgcg aatcgaagcc catggtgtga cgatgaacgc
     4741 gatcaacaac gaccaaaaca tcgccgcgct ggcaccaatc ggaatcgggc gcatgtaagg
     4801 ccccatcaaa ccgcccacaa acgccatcgg cagcaccgca gcgatcaccg caaaggtcgc
     4861 gaggatggtg ggattaccga cttcgcccac agcctccacg gcgatggcgg accatttgcg
     4921 gcctttgttt tgcggcaggt ggaaatgccg cacgatgttt tcgaccacca cgatggcatc
     4981 atccacgagg atgccgatgc tgaagatgag cgcgaagagc gtgatgcggt tcagcgtgaa
     5041 gccgtagagg taaaaaacga gcagcgtcag cgccagcgtg gccgggatag ccaccgcgac
     5101 gatgccgctc tcccgccacc cgagcgtgag ccagatgagg atcgccacgc tgaacacggc
     5161 gatgcccatg tgcagcagca actcgttcga tttctccgcg gctgtctcgc catagttgcg
     5221 cgtgatgctc acgcttacat cggcgggaat gacgctgcct ttgagcaact cgatcttctg
     5281 cagcacctca tccgccacgc tgatcgcatt cgcgccgggg cgtttggcga tgctgagcgt
     5341 cacagcgggt tcgtcgttgg ttttgttgcc gtggaagacg tactgcgtcg gctcttcgcc
     5401 tccatcgacg atctcggcga cttcgcgcag atacaccgga cgaccgctga agacgccgat
     5461 gaccacattg ccgacttctt cggcggtttt gaggaaggca ccggtctcga cgaggacttc
     5521 attgttgccc gtggtgaggc cgccggcgcg gaattggcgg ttcgcctgct ggagcatcgg
     5581 cacgaggccg gcggggctga gattgcggct ggcgagcttc acgggatcga gcatcacgcg
     5641 cacggcgcga cgcgaaccac cgagcagcgt cgtctcggcc acgagcggca cttgtttgat
     5701 cgattcatcc acctgcgcgg ccaaacggcg cagcgtgaga tgatcgtaac gggcgctgtg
     5761 aaaggtaagc gcgaggatcg gcacgtcgtc gatgctcttt ggcttcacga tgggcattgt
     5821 gacacccatc gggatgcggt cgaagttcga acgcagcttc tcggtgatct tcaccaggct
     5881 gcgctccgga tcctcgccca ctttgaagcg cacgatgacg aggctctcgc tgtcgcgtga
     5941 ggtgctgtag aggtattcca cgccgggtac ctcccatagc agcttctcca tgggccgtgt
     6001 ggcgcgttcc tcgatctcct tcgcggtgga gccgggcatg gccaccatga catcaatcat
     6061 cggcaccttg atctgcggct cttcttcacg cggcagcagc accaccgccg ccgccccgag
     6121 caaaacgaac gcaatgacca ccagcggtgt cagcttcgaa tcaatgaacg tcgccgcaat
     6181 gcggcctgcg atgccgagat gcgaagcaga gtagccatct cgctccgtcg agataagatg
     6241 gggactttcg ccgtcgcgca gcgtattcgt agtttgaacg ccgctgttgt tggcgggagc
     6301 gttttgctca tctcgcggga gcgagatggc tactgtactt ttgagctcgc tcatggctgg
     6361 atcgtcacgg gttggccgtc ggtgagtttc gcggtttcgc tcacgatgac ctgttcacct
     6421 tcttcgaggc cggaaagcac ctcgacgcgg ccttcgagca ccttgccagt cttcacgagc
     6481 ctcagcgctg ctttgccatt ttgggtgacg aagacgagtt ccatctgtcc acgcttcaaa
     6541 acggcgtttt gaggcaccag cagcagtttc acctcggcca cgggcacgga gacgcggcca
     6601 aactgtccgg tgcgcaggcc ttcggttgct ggaaggtcga gcttcacatt gaacgtgcgg
     6661 ctcaccggat cggccacggg cgaaatttcg ctcaccgtgc cctcgattac cttcgcgagg
     6721 cgcacgggca tcttggcgcc gagcttcacg cggtcgagga tggcctcggg aagatcggct
     6781 tcaaagcgca gtgaagtcgg cgcttcgatt tcgagcagcg gtttgcccgg catggcgaga
     6841 tcgcccacat cagcgagctt gcgcgtgacc acgccatcga agggcgcggt gactttggca
     6901 tagctgagca tcgtttcggc ctcgctcacg gctcctgtgc cgattttcac gcgagcctcc
     6961 gccgcatcga attcctgccg agtggtcgcg ttgctcgcga tgagctgctt ttgacgatca
     7021 aagtcgcgtt ttgcctgatc gagcatcgcg cgggcctgat cgaccttggc ctggatctcc
     7081 tgcacatcga gccgggcgag caaatcgccc gttttcacca tcgcgccggg cgtggcggtg
     7141 tattcgagca cacggccgct gaccttcgcc tcaacgacgg cgcgttgttt cgaccgcacg
     7201 gtgcccacga cctcctccac cgccgtgtgg gggctccatt tcgcggcttc caccttcacc
     7261 gtgacgctgg gcagtgtcgc ggatggcgcg ggggagtggt tatgctttcc gcaagcggcc
     7321 agcagcagga cggggaagag gcgtgaaagg tgtttcatcg cgtgaagtga atcgtgaatg
     7381 gatcagctcc tcgtgcagca cgagccgccg acaccgagct ttttgaggac cgtctccgcc
     7441 gggcaaaagc cggtgaaggc ggattggagg aggttcacgc cgataaaagt ggtgagccac
     7501 agccagttcg ggctcacata atgcgccagc gcgaggctgg tgaggatcat ggtgcccgcg
     7561 agggcgcgga tggcgttttc gagtttcatg gttttggggt ggtgtggttc aaatcgatgg
     7621 ccggagccgc ttccggtgcc ttcggcagca acacgcagga cgggcagccc tgccgggtca
     7681 tctgcatgcc cgccagcaag gcctcgagcg ccgcgatgag gatgaagagg cggttcggag
     7741 tcatggtgaa gctttattca actgtatgag tctatacgca tatagtgtgg ccgggcaaat
     7801 ggtttttcgg ggacgtcgga cggtctgctg acttcgctca ggcgaaacaa agtagccatc
     7861 tcgctccgcg agatgagcaa aaggctttcg tgggcatggc ggattccgat ttctcgtaaa
     7921 ctggggtggc gctgagacgc tgcctgctca tctcgacgga gcgagatggc taccgtgctg
     7981 acgcatcatt tgagcgctcc aacttggttt tcagacacgc ctacctcccc ggcggctgaa
     8041 acccctgcaa ggcgttgatc gtggctgtgc cgggcttggt gggcacgaga cggagggtat
     8101 gatcgtcatt tgaaagcatt tgggcgaggc ggaggacgaa gggagtggcc aatgaggctt
     8161 tgaaggtgac ttcgtcgagg acggcgggtt tgacctcgaa ggtgaagcgg tcgccggtgc
     8221 ggttgcgctc ggcgcgacgc cagaggtcgg gctcgatgag gatctggccg ctggcgctgg
     8281 tgaagggctt gaaggcatta ccgttgccgt cggggccggt gacgctgccg gtgagtgtgt
     8341 agtcgccgct gtcgctggtc atgacgagcg tccaggtttg cggcacgatc ttctggccaa
     8401 gtgtgatgcc gtggggtgat tgatcgcgcg gattggcgcc tttgccttcc ttggcgtttt
     8461 tggcatcggg ttgcacatag ctcatgagcc aggcattgga ctcgcgggcg ggtttctcat
     8521 cgatcaggac gcggaaggtg cctccatcgg gcgcggtgat gccgtggagg tcgatgcgtg
     8581 tgccggtgaa tttggcggtg aaggagccgt cgggctgctt ctcaggctgg agctgctttt
     8641 cgcggctcgc gggctcgtag ctgaaggcct gcggcacgcg gaagtgggcg aggatgttgc
     8701 tgttgatgat gtgggcgccg tagtcgctct gatgcacggc gtctttgagc agggctggca
     8761 cggccagctt gttcgcggtg aggtaggcgg cccagtcgcg gcggttttca acgaactcga
     8821 cgtggtgcct gcggcagacc tcacgcacgg cggccacgtc ttgatccgcg gcgttctccg
     8881 attggcccca cagggggatg tcccgttcgc gccagtgaat gctgggcacg atgatgtcgg
     8941 cggtggtgtg acggcgcagc tcgatgagga gcttttccaa atcagcggga tcaccaatgg
     9001 tgtaggtgat gatgaggtcc ggctgatcgg ggatgacgag atgccgtgcc cagccgcgca
     9061 gatactgcca cgggcaggaa ttgccgacca tcttgcgata ctcgacctgc ttcgtcttcg
     9121 ggaagcgctt gggcagctcg cgctgccaga actcgccgct gccgagcatg ttcgtgtagc
     9181 tcgatccgat ggcccagatg agaaacgggc ggtccagtcc ctgctggagc ttgtgcatcg
     9241 tcagcgcgag gtgctgccca tcggtgtccg ccgccttcgc cacatactcc gccgcgcctt
     9301 ccggtctgcc tttcgcaaac accggctcgt ccgtgacgag actgcgctcg gcggtgaaag
     9361 ccggaggaga tgggagatga agagccagag cgagagcaag aacgggcggc agggcgaggc
     9421 gatgcatggt tagggaacga tcttgagctg acttcgtttc agcgaggcgc tcgtcacgtg
     9481 cttttcggac gggacaacac cctcgccaga ttttcagtgg acgaaaccgg gttcatggtg
     9541 aactcatggc ccactgtatg agtttatacg catatagtat ctgtctgaaa accctttttg
     9601 aaatcatgcc ctccacacga aataaaaaag cgctgccgcc tgtcaccgac gaggcgctgg
     9661 cgctcatcgc ctcctggttc cgcacgctgg ccgagccaag ccggctcaag atcctccgcg
     9721 ccctcgaaga aggcggcgaa ctgaacatct ccgagctcgt cgaggccacc ggcctcacgc
     9781 aggcgaatgt ctcccgtcat gtccagtcgc tcgctgatgc cgggatggtc ggccgccgca
     9841 aggagggcct cgccgtcatc tgcttcatcg ccgacccgag catcaccgag ctgtgcgaca
     9901 aggtgtgcgg caaccttctc aaacgtctct cccagcaggt gaagaagctc ggtggggcgt
     9961 gagggtcgtt tcaagtttca ggtttcatga tcttccgccg tatgctttgg acccttgtca
    10021 tcgtcgtcgc cgtcatcggc atctggtatg tttatgaggg aagctgggac cggcagcttt
    10081 tcaaagccgc gccgggccgc gtgtgtgcca atctgaatgc cgatcaagcc aatgcgtggc
    10141 tccgcgagca tcccgagacg caggtgctcg atgtgcgctc cactggcgag ttcagcgaag
    10201 gcgctatgcc aaacgccgtg aacatctcca tcggtgacga ggcctttgac gccaaagtgg
    10261 ccgcgctcgc caaggagaag cccgtgctcg tctattgcgc cggaggcttc cgcagccgca
    10321 aagcggtcgc aaggctcaaa gaactcggct ttcagaacat ccagcacatc caccgcggct
    10381 acatgtcgtg gaaacctgac acacagcggt gaaccaaaca cccgccattc acacgaaaac
    10441 ctcccgcacg acctcgccgc cgaggttggt ggggatttcg tcgcggttgt tgtggccgaa
    10501 gacgacgcgg tgctgatcga ggccgagggc gtggagcagc gtggcgtgga ggtcgttcgg
    10561 gccgattggg cgctcgacgg ggacaaagcc gagctcgtcg gtgctgccga tgatctggcc
    10621 gcctttcacg ccgccgccgg cgagccacat ggtgtagccg gcgggggagt ggtcgcggcc
    10681 gcgtttgtca ccggtgactt ttccctccgt ggtgggcgtg cggccaaact caccgcccca
    10741 gacgacgagg gtgtcctcca gcaggccgcg ttgtttgagg tcggtgagca gtccggcgat
    10801 ggggcggtcc acagcgcggg actggctggt gtggttcgag cgcaggctgc cgtgcgcgtc
    10861 ccagccgccg gggcggagct ggatgcagcg cacgcctttc tccaccagcc ggcgggcgat
    10921 gagacactgg ctgccaaact cggccgtggc gggatgatcg aggccgtaga gcttttgcgt
    10981 ggcggggtcc tcgtgcttga gatcaaaagt gtccggcgcg gaggtctgca tgcggtagcc
    11041 cagctcgtag gaggcgatgc gggcctccag cagactcagc ggcggatacg gcaagcggcg
    11101 cgcccgcaaa aacgagcgtg agaagaaggg cggacgcggc aggctacatg cgtggcggag
    11161 aaattttaca aacgcagccg agcccccggt catgcgtctt gggcggcagc aagcctcctc
    11221 accagtcggg atggtacttt ttcagccagg ggtcttcggt ttcgatgagg cgctggtgaa
    11281 gtcgggcctt catggcggcg aggcgctcgg cgtgggcggg gtcggtggcg aggttgcgga
    11341 gttccgagga gtcgctttcg aggtcgtaaa gctcgaactt ggggcggttg aggaaggact
    11401 cgacggtgcg cgggccgatg tgggtggcct tggtctcgac gatgtgacgc cagaggcgtc
    11461 ggctgaaggt gtccacgggc agggggaact cggccttcca agtcaggttc cagatgagct
    11521 tgtagcggcg ctcacgcagg gtgcgcatgg ggtagtagct gtagatctcg tgggcgacgt
    11581 ggctcagcag cacctcgtcc cagccttcgg ctttgggttg atcgaggatg ggcaggaagc
    11641 tgcgtccgtg ggtgcctttg gcggggtctt tgactcctgc ccaatcaagc agcgtgggcg
    11701 tgatgtcggt gaaggcgatg agggcttcgt ggacgatgcc ggcagggccg ccgggctttt
    11761 tcacgatgaa cggggcacgc acgccgggtt cgtattgcgt gcccatggcg ccaggctcgg
    11821 aggtgccgtg gtcggaggtg aagatgacga gtgtgttgtc cgtgcagccg tgctttttca
    11881 gagcctccag ggccaggccg aggccgtggt cggcgcggct gacctgctgg tagtaaccgg
    11941 ccagttgctc gcgcaccatg ggggtgtccg gcagaaaacg cgggacttcg atccgggcgg
    12001 gatcgtaggc gatgggcgtg tagccgttct tcgcttccac accccaggcg acaccgttca
    12061 tgccggtgat gccgttggga tgtggatcgc cgagggcgag ggtgaggaag aagggctggt
    12121 cctcgcagga ggcgatgaac tcgtcgcagg ctttgccgag gccgacaacg tccttggaac
    12181 tgatgcggta agcgccgacc tcgccttttt cctcagcggt ggtgaagtcg aaggggtatt
    12241 tgtccagcgg ggcgatgtgc tgcttgccga tcagcgcggt gcggtagccc tgcgccttca
    12301 gtttggcaaa gacggtggtg atgtccgggc gcaggcgctg gttgtgctcg tgcggggtgt
    12361 gggcgtactg gccgttccga tgcgtgagca cgccggtgaa aatgacggcg cggctggcgc
    12421 tgcatgaggc cacggtggcg aaggcctggc ggaagcgcac gccctcggcg gcgaggcggt
    12481 ccatgtgcgg cgtctgcacg accttgttgc cgtagcagcc gaggtcgaac cagttttggt
    12541 tgtcggtgac gacgaacaag atgttgcgct gcggttgggc ggcgccggat gctgaagtgc
    12601 cgaatgcagc cagcagcgat gcgaaaagaa gaggcctcat gaagaacttt gagtgagaag
    12661 cagaatcact tcaccagcgg gatgtctttc cttgctttga gccgccccag cgcgtgctcg
    12721 ccgatgagcc agacttgcag cgtgccgggt tttttcagca gatcggcggg aatcgtcaga
    12781 gcgccgcttt ttcgctgagc ttcggtcagg aaaatggggt ggcggccata gctgtcgccg
    12841 gagttccaga gatagacggc gatgacgtcc atgttttcgg agccgaatcc gaccttccat
    12901 tctttgccca aggagacgct ttcgccgggc aggcggaggt cgagatcgca caccgcaaac
    12961 tcgcatgcct ggcttttcga gccgtcggca tggatgacgt gcgccgagta gtcgccgcaa
    13021 acctcaaagc ggcgctcgtg caggccgggg ctggtgagcg cgatttcctc caccacgtcc
    13081 ttgccgcgct ggatgacgag cgctttaacg ccgagctggt cacggtccat gacgtggaat
    13141 ttcacaagcg cgcctttttg atagggaacc cagtcgccga gatcgagcag cagggcgcgg
    13201 ttgatcttgg cgggtgtggc gtcgaggtcg aagttgggga aaagcagcgg ctcggaacgg
    13261 ttggcgccgc gccaggcttc gcggtcggtc acgcgcagca gctgcttgtt ccttgcggcc
    13321 agatgcgcgt tgaagttggc gacactgcgc tcggtggtcg ccgtggtggg cgggcggctt
    13381 tcctcgacgc ggatggagac gaggcggcat tctttgtccc gattcacagc ggtgaccatc
    13441 tcgacatgcg agccggaggt ttccgtggca tggggcgtgt agatcacatc ccccacctgc
    13501 gcggcctcgg cggactgcgg ctccaccaag atgatgcccg tgcccaccgg cggcccgtag
    13561 cggcggctga cctccgggat gccgcagccg agcgcgtagc tggtgtaggc cgagcagacg
    13621 ctgccgtagt aagcggcggc gttggcgacc ttgccggtga agttttccgt gtagagcacg
    13681 ctgtgcggat tctccacggc cgccaaaaac gtgcgcagag cgatgtcgaa cccgatgtag
    13741 cgtccctccg agcgcacgct ggagtagggc acgccggtgt attccttgcc cttctcaaaa
    13801 tagccgccct tccggttcgg cagcgtgtcg gctacgggcg accacttcac ctgcgaaaga
    13861 atgcgcgcat gtccggcggc cttctcctgc caggcgagcg aggccgggtc cgtctgcgcg
    13921 gaggcaggcg aaagagcgaa gaagaatgtg gtgatgacgg tttggagtgg gcgcaagaag
    13981 gactgcataa ggaaaaaagc tggcaggtag ggatgtgttg aagggagtgc aaaagccagg
    14041 ccgtttgtcc tgtcatgtcc gcgcgttggc ccttctcttt agccatcatc catcaacgga
    14101 tgccctcttg atgaaattgc ttttcccaac gtttatgctg tctgccatga tgacgctgat
    14161 cacgccacct gcgtgtgccg gcgagccaaa agtctttcgc actttcatgc cggaggccgg
    14221 gccgagcgcc ttcggcgtgg tgctggggcc ggagcttgcg ctgtgttaca gcggcccgaa
    14281 ctgacccgcc tgcgcgacac ggacggcgat ggcgtggcgg atcactacga aacgctgaac
    14341 gatgactggc aggtgacgga caactggcat gagttcacct tcggcctgcg gcgggatgcg
    14401 gcgggcgatt tcatcctcgc caccggtttg cccgatgtgg ctggcgaaat cagcacgcgc
    14461 tttccgcgcg agccgctgcg cttggaaaaa gtgcatgagg aggccagcct tcaccaggcc
    14521 gctatgaggg ctgggttttg aaggtttcgc cggatggaaa gttgaagccc atcgcctccg
    14581 gttttcgcgc ggcttcggat ggtcgaaaat ggtgccggtg gaaatcgcca ccatgcagct
    14641 cacccctgag ggtttccgcc tgaccttcac cgctgcgatg gatgcggctc gtttgcagaa
    14701 tgtgaaaacc tatcgcctcg catccacata gcagtgcgtg caggtctggt tgcacttccg
    14761 gcccgcgttg atttgcagcg tctgaaacgc actgcgagtg agcgggatcg cgtgctcggc
    14821 gagcttttgc tgaaaacggt tcatggtggg aaatcagcag cagctcgtgc catcgccggt
    14881 ggtgcggcag ttcgcggctt cggtggtggc gttgcaatcc tggcctttgg tctcctttgg
    14941 gtggcgaagc ctcatgctgg agcagtcgaa gggcggtgcg gagtccagcg gcacctcggt
    15001 gcgcggctcg atgaactcga agcaggcgca atacggctcg ttttgcagca gtttgaaggt
    15061 cttttcgcac acggcgtagc gtttgccgcg ctccatggcg tggccgtcgt cgtcctgcac
    15121 tttcaagaac ggccctttgt agatgacggc ctggcttcgg tcgagatacg gcccctgtcc
    15181 cgccttccat gcctgcacgg tcaggctgcg gaattcaatg ccctccaccg tctgccacgg
    15241 ctgctcgtcg cgtttgagga tctcgatgcc gtgaaagccc gcgtcttgaa acgctttcaa
    15301 aaagccctcc tcggtgagcg ctccgctgat gcagccgctc cacagcgcgg gatcccgttg
    15361 cttgtcctcc ggcacggact cgtcgctcac gatgtcagag atcaccgcgc gtccgccgac
    15421 tttcagcacg cggaggattt cctcgaacaa ccgccgcttt tcccgcgcct cgaccagatt
    15481 gagcacgcag ttcgacacga cggcatccac gctctccgtg gcgatgagcg gatgcttcac
    15541 acgcagctcc tccgccaggt cgtgggcggc gagaaacgag gccgtatcgg cgatggggcg
    15601 ctttttcagc tccgagtcga gccgctccaa atcaagcgcg aggtcctgga tgcggccttt
    15661 gcgaaattcg acattcgcat agccggtgcg ctcggcgacg atgggcgcgt tacgccgcgc
    15721 cacctccagc atgtcatcgg tcatgtccac gccgatgacc ttgccagcag cacccacaac
    15781 ttgttaggcg atgaagcaaa tcttccccgt gccgctgccg agatcgagca ccgtctctcc
    15841 gggccgtaga tgcttcgagg gatcaccgca gccgtagtcc ttctcgatga cctcggcggg
    15901 gatgatcttc aaatactgcg gatcatagct cgccagacag cacaatgcgg cttcagatac
    15961 cttggcggct gccgcataac ggtcacgggt ggcagattcg gcggtgggaa tggtcatgaa
    16021 gtggagaatg ggtcgcggtt atggcaaaag aaggcaagtc tggatggctt cggaacagtt
    16081 ttattccctc tgtcagcaag caaccctgat gctctgctga tttatttgtt gccaagattc
    16141 ccaccatgaa aacacgcctc agcgcccttt ttctcgcctt caccgtcttt gctctcgccc
    16201 aggacgcgag cgtcaaatcg ggcatcaacg acaaattcct cgatccgaag ctcaatgtcg
    16261 aagaatggac gaagaagttc gaaacggaga gccgcgagat cttccaccag cgcgagaaga
    16321 tcgtcgccgc agccggtttg aagccaggca tggccatggc ggacatcggc gcgggcacag
    16381 ggctcttcac gctgcacttc gcgcaggccg tcggtgaaac cggcaaggtc tatgccgtgg
    16441 aaatcgcgaa gaacttcctc gaacacatca aagcccgcgc cagcaatgcg agcgcgtcga
    16501 atgtgcagac catcctctgc accgaaaaaa gcgtcgagct gccggaggcg agcattgacc
    16561 tcgccttcat ctgcgatgtg tatcaccact tcgagtatcc gcaggccacc ctcgccaccc
    16621 tgcacaaggc tttgaagccc ggcggagagc tggtgctcat tgatttcaag cgcatccccg
    16681 gccagacgag cgacttcatc atgggccacg tccgcgccgg tcaggaagtc tttgaggccg
    16741 aagtcaccgc cgccggtttt caaaaagtgg aggaaacaac cggcctattg acggagaact
    16801 attttgtgaa gttccgacga aaatgaccgg gatcccggca ccacgaggat gcggtttgtc
    16861 ccggcaagat acgatcctca tcattcaagc atcgcgcgcg cctctgcctg cacggcaacg
    16921 cggagagcgt tcggggaaag gactcgggcg tgacgtccgt ggctgagcac ccagcgagtc
    16981 acctcctcca ggcccggcag ctccgcctgg aattcgatct cgttgttttc atttcccacg
    17041 gcaatgatgc tctgactcgg gtgccagtga cgctcggcca caagacgcgc ggcccatccg
    17101 gtgaagcgga tgcgaaccgt gtggcgctcc cccgcaccgt cgtagctcca gacgccgaaa
    17161 cctccgcgca aatggccgcg cgcgtcgaag ctgccatccg gctcaaatgt ggtgtcaagc
    17221 acgcgcaggt tggtgattct ctggagggcg aaggtgcgca gcgcgccgcg cgaagggtcg
    17281 tgggcgatca aataccatcc gtgctcgatc tggcccacat ggcggggatg cacggtccgg
    17341 ccttcgggcg cggcggctgt cagcttgtga taatcaaatg ccaccacacg acgcgcgcgc
    17401 acggcttcca ccaggtcgcc gaaaagtctc acatccgccg caagcacgcc cgcagccttc
    17461 acggagaagg cctgatccag ctcatgccac tgaatggaga cctcccccgg gcaggcatcg
    17521 gcgattttgc tgaagctctc cgccagcatc cgctccaggc gggtgccgcg caaaggctca
    17581 agggcatgcc gcgccaggaa gagcgccacc aagtcagagc gggagagcct cagcatgggg
    17641 aactcattga cctcccgcgt gtagtggtag ccgtgccgca gcgcgtcgta ctccagcgga
    17701 aggtcgagct ggtcgcgcat gaaattgatg tcccgctgga tggtcttcgg agtcacctcg
    17761 atctcgcgcg ccagtgttgt gcaattcggg aaccgtccag cccggatgat tttatgaatc
    17821 tccagcaccc gccacatggc cgggcggctg gcgccggccc tcgcctcatg cgtgcgcgtt
    17881 tttctggctt tcactgtgcg ggtggtggtc ctggacatga gcagtggaaa atatcgcgga
    17941 tttttgtttc tgtcccccca taaatgtccc accccctgtg gcagggtgcg gcgcttatga
    18001 atccacacct cacagaaatc gcctacatcc tcgaccgctc gggctccatg caaaacatgc
    18061 gagaggctgc cgtggccgcc ttcaacaact tcgtcaaaac ccaactcgac gtgcccggcg
    18121 acgcccgcct caccctcgtc cagtttgacg actcctacga ggtgcatgtg gccgccaaac
    18181 ccgtccagga tgtgcccgaa ctcaccgccg ccacctacca gccgcgcggc agcaccgcct
    18241 tgctcgacgc catcgggcgc acgatcaaag aaaccgaccg ccgtctcaaa accctgcccg
    18301 agacggaaaa acccggcaaa gtcatcttcg ccattttcac cgacggcgag gaaaacgcat
    18361 cccaggatta ctcggcgaag cacatcagcg acctcatccg tcttttccgc ggtgaaaagg
    18421 gctgggaatt tatcttcctg gccgccaatc aggatgcgat tgccaccgcc tcggccatgc
    18481 gcatggactc cagcctcagc agcggagtgg ccttcagcca caaaggcatc aagtccacgg
    18541 gcagcgcgat ggcccgcaaa gtgcgagcca tgcgcatgaa aagcagcggc agcatggacg
    18601 cgcaggcgct cgaagacgac gccaagtcca tgtcggagat catccaggag gaggaaaaca
    18661 aacccgacga ccaagcctga gccgaaatct tcgcatcttc cgaccatgaa ccgccgacgt
    18721 cgctggcagc aacgccgcca cccgctaccc tcgtcatgct cagtcctgcg aatttgcgcc
    18781 tggtgccacc tgctcggagt cgcgtgttcg ctggcctggg gactgccggt ggcggccctc
    18841 gtcctcgccg ctgccgcagt cagttgcgcc atatgcgggc gcgggcgcag tccggcggct
    18901 ggatgaaaaa cgggccggcc catttctggg acggcccgtt tgtctgattc aatgaaccgg
    18961 cgcgccggag cttacagccc cttgccgaca atgaacttca ccaggtccac gacgcggttg
    19021 gaatagcccc actcattgtc ataccagctc accagcttga agaacttgct gttcagctca
    19081 atgccgctgc cggcgtcaaa gatggaactg tgctggtcgt gaatgaagtc cgtgctgaca
    19141 acctcgtcgg cggtccaggc caggatgcct ttaagataag tctcgctggc ctttttcatg
    19201 gcggcgctga tctcggcata gctggtctcc ttgacggtct tcacagtgag gtcaaccacg
    19261 gaaacggtgg gggtgggaac gcggaaggac atgccggtga gcttgccctt gacctccggg
    19321 atggcgaggc cgacggcctt ggcggcgcca gtgccgctgg ggatgatgtt gatggcggca
    19381 ctgcgcccgc ctttccaatc tttcttgctg ggaccgtcca cggtcttctg cgtggcggtg
    19441 taggagtgga ccgtggtcat gaggccctcc tcaatgccga agccctcctt gaggaggaca
    19501 tgtaccacgg gagccaggca gttcgtggtg caggaggcgt tggagatgac gtggtggctg
    19561 gccgggtcat acttctcatg gttcacaccc atgacgatgg tgatgtcctc gtttttgccg
    19621 ggagctgaga tgatgacttt tttggcaccc gcgctgatat gacccattgc cttgtccttc
    19681 tcggtgaaaa gaccggtgga ctcgatgacg atgtccacac cgaggtcctt ccacggcagg
    19741 gcggacgggc cctcacggac ggcgaggcat ttgatctcat gcccgtccac caccaggatg
    19801 tccggttcgg gatccacctg cttgccggat ttctcggagg caaccctggc tttctcgatg
    19861 gcctccgggc tgcatttgct ggagacctcc tccttcgccc tgccctgcgt ggagtcatac
    19921 ttcacgaggt aggccagatt gtccgcgggc acgagatcgt tgacggccac gacttcgatc
    19981 tccttgccaa gaaggccttg atcacagatt gcgcgaaaaa ccaggcggcc gatgcgcccg
    20041 aaaccattga tgccgatttt gaccatttga tgagggagtg tgtttggggt tttccagcag
    20101 catgcgtgcc ggggcgggat gcttagtgcg ggaggtttcg ggcgtcaacc ctggagcagg
    20161 cgcggctctg ggcggagcat tcgaatcccg gcttcatgga ctggtcagcc tagtgttcca
    20221 tggagagccc ccaaccaaca ttttattttc atttgtgaaa aagtgcgccg cttttgcgtt
    20281 taggcggttg atgaaaatct cccccctgcc cttcgctctg acccttgtcc tgtccgccct
    20341 tgcgggtgcc catgccgcgc cagacacggc ctcggctgca cgccagattg actccatcct
    20401 cgaagccgat tggaagaaaa acaatcttca aggaaacccg gccgccgatg acaactcgtt
    20461 cgtccgtcgc atctatctcg acacaatcgg ccgcatcccg accgcgcggg aagccgggga
    20521 attcctctcc tccacagcgc ccgacaaacg cgccagactc attgacaagc tgctggcctc
    20581 agatggctac acacaacact ttttcaatta ctggtcggat gttctccgca tccagaccaa
    20641 cggcccccag gccggaccgg tgacaggcgc ggcgtatgcc gacttcatca agcagtccct
    20701 gcgcaccaac aaaccctacg accagatggt ccgcgacatg gtggccgcgc aaggcaaggc
    20761 gtgggacaac ggcgccatcg gctactacat gcgcgaccgc ggcatgccgc tcgacaacat
    20821 ggccaacacc gtgcgcgtct tcctcggcac gcgcatcgag tgcgcgcagt gccacaacca
    20881 cccctttgac aaatggtccc agatgcagtt ctaccagatg gccgcctaca cttacaatgt
    20941 ggacacccag gattattatg gcggctcgct ggaggatgtc cgcgagctgt tgcgcgagcg
    21001 cgaatcagaa ttgcgcgcca aattcaagga gccccagcgc ccgcgccgcg accgcaagat
    21061 gagcgacgcc gaatgggcaa ggctcgaaaa agaataccgc gaccaggccg ccaaagtcag
    21121 gaaggaatac gaaacagcgc gccaggccat gcgccaggag cagcgcaatt accaggaggc
    21181 catgaccgac gtgcgcaaca ccatgcgcta caccgccgtg gacatgagaa atcgcaacct
    21241 cacgctcccg catgattacc aatacagcga cgccaaaccc cgttcaacag tccaggcttc
    21301 tgtgatgatg ggccacgaat gcgcgaccca gccgggagag actcccctcc aggcatacgc
    21361 ccgctggatg accagcaaag acaacccccg atttaccagc gtcatcgcca gcagactgtg
    21421 gaaaaaagct ttcggactcg ccctcatcga accgctggat gagctgatgg acacatccac
    21481 tcccatgatt cccgagcttc aaactcatct tgaggggctg atgaaaagcc tggactacga
    21541 catgaaggcc tatctgaggg tggtgttcaa caccagcgcc taccagcgcc aggtgacccg
    21601 tgaggaggtg ccccctggag tcgcctatca cttcaccggc ccgcttctcc gccgcatgac
    21661 cgccgagcaa atgtgggact ccttcgtcac cctcatcaac ccgaacccgg acatgcccaa
    21721 tctgcgcctg cgcgaagacg ccgagcagcg cattctccaa gccaagaaaa acgcggacgg
    21781 agtggacgcc ctgagcgtcg aggaggctct gcgcggcatc aagctcagcg cggctgttta
    21841 tgacaagaac cgcgagcgca ccgaggccgc ccaaaaactc tatctggaag cgcgcatccg
    21901 ccacaaggaa ctccaggacg aggcggacag cctcaaggca ggccccgaac gcgacgccct
    21961 gcttgtcaaa gtcgccgacg cgaaaaagaa atccgacgat ctgcgccgcc aagtcaacga
    22021 catccagaac gaaggccgcc gcacctccac ccaggagatc atcgtggccg gccacaaaaa
    22081 gctgtatgag gtgacgaccg gcaaaccctg gcagcccgtc tccaaagccg tcaaagactc
    22141 gactgacggc agtgagcctg ccatgatggc gtctgacacg atgatgatgg cttacggagt
    22201 gcgcgcggaa cgggtcacga ttccgggcta cgaccgtccc gaactctcca aagacgagcg
    22261 caaagcccgc gaggatgcca tgcgcgagga attctctgag gaagcccgct tctacggtct
    22321 gccggaaaag gagttgcggg attacttccg ttcccgcgag acgcagaacc gcacctatgt
    22381 gcgcgccgcg gaggagcaaa gtcccgcgcc gcgcggacac cctttgcgcg acttcggtca
    22441 aagcgaccgc gagaccattg agaacgccaa ttacgacgcc agcgtgcctc aaagcctttt
    22501 catgatgaat ggcagcctcc tgcccaacat cctccaccgg cacagccagc tcatgctgac
    22561 gatcaacaaa gcccagtatc ccgacgacaa ggtcgaagcc gcatacatgg ccctgctcgc
    22621 ccgcaaacca acctcaaaag aaatggaaac ctggaacaag gccgccgacg ccggtctcga
    22681 caaaatcgag gaccttgtct atgccctgct caacacccag cagttcatct tcattcagta
    22741 aaccagcctc caacacctca acccgccccc caacatccct ccgcccatga aacaagaact
    22801 cgcctccaaa ctcctccgtt cgggtgaaat cacccgtcgt gacttcgccg caaagacagc
    22861 ttcctccctg ctcggcgtcg gccttctcgg ccattcgctc tccagccgct cctacgccgc
    22921 cttcgaacag tcctcgaaat tgcgccagac ggccacggcc aaaaacgtca tttacctcta
    22981 tatgagcggc ggccagaccc atctcgacac ctgggatcca aagccgggaa ccgagaccgg
    23041 cggtcccacc aaggccatta aaaccagcgc caacggcgtc cagctcgccg aaaacctgcc
    23101 cctcaccgcg cagcacatgc accacggcac cgtcttccgc tccctgtcat ccacccaggg
    23161 ggcgcacgag cagggcaatt acatgatgca caccagctac gagctgcgcg gcaccatccg
    23221 ccaccccggc atgggcgcct ggctgaacgt cttccagggc ggcggcaaca gcaccctgcc
    23281 caagtacgtt tacgtcggca atgacagccg ccatcccggc gccggcttct tccccgccgc
    23341 gcaaagcccg ctcttcgtca acaaccccga ggccggcctc aaaaatgtga agccgcattc
    23401 cggtgtgaca gaggagaaat tcaacgcgag actcaaactc gcgggcgagc tggacgccga
    23461 tttccgctcc accttcccgc accgcaacgt caaggcctat gccgagatgt atgacgacgc
    23521 catcgccatg atgcgctccg aggatttgaa agccttcgac ctatccgagg aagccgacga
    23581 catccgcgcc gcctacggcc gccagtcctt cggccaaggc tgcctgctcg cccgccgtct
    23641 cgtcgagcgc ggcgtgcgct ttgtcgaagt ttccctcggc ggctgggaca cccacaatgc
    23701 caacttcgtg cgcgtccccg aactttgcga gaccctggat cgcggcctct ccgccctgct
    23761 tgccgacctg cagaaccgcg gcctgcttca ggaaaccctc gtcgtcgtcg ccactgagtt
    23821 cggccgcacc ccggacatca accaaaacgt cggtcgcgac cattatccga aagccttctc
    23881 cgcagccatg ttcggcggcg gcatcaaggg cgggaatgtt catgggcaaa ccgaccccga
    23941 aggccgcgaa gttgtcgccg acaaggtcaa aatccaggat ttcaatgcca ccatcgccta
    24001 tgcgctcggc ctgccgctcg accaggtgat ttacagcccc agcaagcgcc ccttcaccat
    24061 cgccgacaaa ggccacccgg tcacgggagt gtttgcttga cgcccccagt acaaaagcag
    24121 tctcatcccc aggagcggcc cgatgacggg ccgctctttt tatttccttc ctcacgttcg
    24181 ggattgtccg cgctattatt tcggttattt tagctatcaa taaaatcaag aatttcagcc
    24241 tgctacattt ttcatgcctg acgccgaaat catcaaaccc agacccatgc gcgcccgcgt
    24301 gcaggaaaaa gagaacccct ggaacgacgc cacagggatc accttcctgg ttctcgccac
    24361 catgtatttg ctggcgctca tctcctacga ccccggagac cttccggctt ggtgtcatct
    24421 cagccccgcc gacgagccaa atgccgtcac gcagaacttc atcggcacag ccggagccct
    24481 ggcggcgggt tactcccttt tcctcgctgg cctgtccgcc tacatactcc ctttcagcat
    24541 gacttggttc ggcgtctgca agctcgcctc ccatgtccgc atcaccggac gctcctggtt
    24601 cggcgtcctg ctcatgatcc tgagcatctc cgcgcttctg gaggttcaaa acatcatgaa
    24661 cgagcgtgac gagatcaccc cccttggcgg cggtggcggc atcggctacc tgatcggtgg
    24721 cagtttccta gccgggctgc tcggcaccgc cggctccacc atcgtgctct ccggcatcta
    24781 cggggtcggc tttttcctcg ccagcgggct gcacccgctg ctcgtgctcc aggacatccg
    24841 gcgtgagatt caggagttta aagagcgctg gcgtgtccgc cgcgaactcg ccgcccggca
    24901 ggctgcggaa cccgcgccgg cagccggcac cgggatcatc cgccgcaaac gccccgccaa
    24961 atccgagacc gccgacgaaa cccctccatc aggcccgatg gtcacacccg aactcggcct
    25021 cacctttgag cctcagcgcc cgaaaatttt cgacgccaac gaagttcgcc accccaagga
    25081 cgccgccgac aagcccaagc tctccgaagt ttgggagaaa aaacgcgcgc aaaaactcga
    25141 gcaggcaccg catgggacac tcggcagcct ggccgtgctg ttcaaggact acaaactgcc
    25201 tgacactgac ctgctccact ggcccgacga aacccagcgc gtcacggccg acaaaggcga
    25261 gctgctggcc gtgcaaaaca ccatcatccg atgccttgcc agcttcaacg tcaaggtgga
    25321 gcccggcgac atcacgcgtg gtccggcaat cacccgctac gaggtgcggc cggaggacgg
    25381 tctgcgcgtc gcccgcatcg ccgcgctgga caatgacatc gcccgcgcga cctgcgcgga
    25441 gcggatcaac gtcctggctc ccatcccggg caaggacacc gtcggcatcg aactggccaa
    25501 ccgcgaaaaa atcatcgttc ccatccgcga gctcctcgag gatgaaatct tctcaaatgg
    25561 caaagccagg ctgcccatcg cccttggcaa ggacgtctat ggcaagacca tcatcggcga
    25621 ccttgccgcc atgccgcacc tcctcgtcgc cggcgccacc ggctccggga aatccgtctg
    25681 catcaacggc atcatcacca gcctgctgtg ccgtttcgcg cccgacgagc tgcgcttcat
    25741 catgattgac cccaaggtcg tcgaaatgca gaactacgcc gacctgcccc acctagccgt
    25801 ccccgtcgtc accgacccga aaaaagccct gctcgcgctg cgctgggtgg tgaaggaaat
    25861 ggagaaccgc tatcaaatct tcgcccagga gggctgccgc aactttgacg ccttcaacac
    25921 acgaaaccgc aaacgcgcag ccgcgagcca ggcgcgcaaa gcggccgccg ccaaaaacca
    25981 gccgcccatc tccgcaggca cggatgacgc ccgcgcctcc gccgccttcg ccaaagtgct
    26041 ggagcccgac accgctggca gcgccgccac tgcggatggc tccacacctg aaaacgagac
    26101 ggaaatcctg cccgtggatg caagcggcat gtggaccggc gattccgccc cgcccccgcc
    26161 ccgccgccac gaggtggtca tccccgacac cctgccctac atcgtctgca tcatagacga
    26221 gctggccgac ctcatgcaaa ccgcgccggc ggacattgag gtcgccatcg cccgcatcac
    26281 gcagatggcc cgcgccgccg gcatccacct catcgtcgcc acccagactc cgcgcgccga
    26341 tgtcatcacc ggcgtcatca aggccaacat ccccacccgt attgccttcc aggtctcctc
    26401 ggcgctcgac agccgtgtca tcctcgaccg caaaggcgcc gaaaacctcg tcggcaaagg
    26461 cgacatgctt tatgtgcccc ccggcggcgc ccagcccatc cgcgcccagg gcgcgctcgt
    26521 caccgacgac gagatccacg ccgtcgtcga gcactgcgtg acccagggca aacccgtctt
    26581 cgacatcaaa gtggacgatg aaaccggcga gatggacggc gaggaattcg aggacagcgg
    26641 aggcggcgtc agcggcgagg aggaggaaac attggagaaa tgcctcgaag tcatccgcca
    26701 ggaaaaaaaa gcctccacat ccctgttcca gcgccgtctg aaactgggct acggccgcgc
    26761 cgcgcgcatg atggacatcc tggaggaccg cggcatcatc ggccctggag aaggcgccaa
    26821 accgcgcgaa atcctcgtgg acatggacat gatctgaatc cgcccgatca cctccgtccg
    26881 gcagttggtg cgaggcagac cactggcttt atgaaacgga taatatcccg tttcatcaaa
    26941 cacgttccgc atttacgact cactcaccat gacccagctg ccccgcctcc tgcccgccct
    27001 gctcatcctg accgcctgcg gtgataagaa cccgcctgtt tccggcgcgc aacctgccgc
    27061 tcctgcggca tccccggcca agccagccgt caccagccag cccccctctg caggggcgcc
    27121 cgcatccccc aaaagaatct ggtggaccgc cgaaatgctc cacaccgaga tcaaatatca
    27181 caatcccgat tacgctggcg gcggcgagtt tgccattgag cagggcgagg tgctcgcgct
    27241 gaatctgcgc gagtcccaag tgaccaacct cgtctttctc gagcgcctca gtccgctggc
    27301 gctggacctg agcgccaccc ccatcaagga catcaggccg ctcaaaaaca tgaggctgca
    27361 ggagctttat cttgaggaca gccccgtcac agacctctcc ccgctgcgcg gcatgccatt
    27421 gcaaaaactc tacctcagcc gcacccccgt cagcgatctg agcgcgctag cgggcgcgcc
    27481 ccttgtcgaa ttaaacgccg tggaaacccg cgtcagcgac ctatcccctc tggccggctg
    27541 cccgattcag atgctctggc tcactggcac tcccgtcgag gacatttccc cgctgaaaaa
    27601 cgttcccatg atctctctga ccctgcaccg cagtcgtgtg agagaccttg gcccgctcgc
    27661 cggcacaagc ctgcgccgcc tccacatcgg cgagactccc gtgaccgatc tcactccgct
    27721 caagggcctg ccgctcaccc gactcgtctt cactccggcc agcatcaaat ccggcatcga
    27781 aatcgccaaa gccctgccgc tggacgaaat cggcacacgt tttgacgagg aacgcgatct
    27841 cgtcccgccc gccgtctttt ggccgcgttt tgagaatggg caatgagtca aatcgaacac
    27901 cggcgggatg ggaaacgaac tgcgcgcgga caaagaacag ttgaccgccc ttgtcatgcc
    27961 cagatattca cgcccacctt tgcgctctgc cgcgtcctcc ccccacaggc accccacggc
    28021 acacatggcc gtttccaaaa aaaaatctcg aatcgcaaaa cccgcccgca aagccagtcc
    28081 ggctgcggcg gcgaatcatg ttgggaagaa gagcaaaccc ggccgcggac gcccccgaaa
    28141 aacagcgccc gccgcgcctg cgggcaagac gcaggagagc ggctctcaaa ttcccgaaaa
    28201 gcgcaagcgc ggccggcccc gcattcatcc gcccagggag aagctgcctc ctgaacccgc
    28261 gacaggaccg gatgggcagc ccctcaaacg caagcgcgga cggccgcggc tttatccgcc
    28321 tagggaagcg gtcaaaccac agccgacgct cggtcctgac gggctgccgg tgaaacgcag
    28381 gcgcggcaga ccgccaaagg gcagccagcg ccaaaccgtc ccggtcatcg tttatgacga
    28441 catcaactcg gccgagatcc agtcccggct gcgcaagctg atcataatcg ccaaagagca
    28501 ggactccctg agttgggatg atgtgaacga cgcactgccg cacgggatcg tcacccccgc
    28561 gctgatggac gagatcatca cccggctgca atccctggag attcgcattg aggactcgtc
    28621 ggccagcccc cccgtcagcg tgctcgccgc cgacctcgcg gcgcgctcct tcgcggacga
    28681 ggcccgtgaa tccatgcgag aagcctcgaa ggccgacacc atggacgatc cggtgcggct
    28741 ttacctccgc cagatgggcc aggtgccgct gctgacgcgc gagcaggaaa tcgagatctc
    28801 aaagcgcatc gaaaacgccg acctgcgaac ccgcgaatgc ctgcaaagcc tgggattcgt
    28861 tgccgctcat cacctgaccc tcgccgagtc cctcgccgcg gggatggaaa gattcgaccg
    28921 cctcgtcata gaccgcaaat tcccggagaa agagagttat ttccgcaaac tgcgcccttt
    28981 gaccgcccag gctgaggaac tttgccgcaa atgcggcaag ctgcgcgagc gcgccggcac
    29041 gctcacaggc aagcgccggg cagacgcact ccaggaattc caaaactcac gcgaggcact
    29101 tttcaaagtc tgcgccaagt ttttcttcaa acccaggctc gacgaggact tcatcagcct
    29161 ggcgcagaaa aaactccacg aacttgagca gctcgccgcc cattgccacg cccatccgcg
    29221 caatgccgcc gccgcggagg cgctcaggca gttcgaattc caggcctggc agaccgtcga
    29281 ggaataccgc gccaccatcg aggaaatacg ccgctgcgtg gcggaatcca ccagggccaa
    29341 gaccgagatg atcgaggcca acctccgcct ggtcatctcc atcgccaaaa aatacaccaa
    29401 ccgcggcctt tccttcctcg acctcatcca ggagggcaac atgggcctca tgcgcgcagt
    29461 cgaaaaattc gaataccggc gcggttacaa attctccacc tacgccacat ggtggataag
    29521 gcaggccatc acccgcagca tatccgacca ggcgcgcacc atccgcatcc cggtccacat
    29581 gatcgagacc atcaacaagc tcatgcgcgt tcacaaacag ctcgttcagg aactggggcg
    29641 cgaccccact ccggaggaga tcgccgagga gatccatctc cctgtcgagc gcgtcaacac
    29701 cgtgctgcgc atggcccagc agcccgtctc catgcagacc aaggtcggcg atgtcgaggg
    29761 cgacaccgaa tttggcgact tcatcgagga caaggacgca agcgacccca tggaactcac
    29821 cgccaaaaac ctcctgcggg acaagctcaa ggacgtgctc gacacactca acccgcgcga
    29881 gcgggaggtg ctcgagcagc gcttcggact catggacggc tccggccgca ccttggagga
    29941 ggtgggccgg caattccagg tcacccgcga gcgcatccgg cagattgaag ccaaggccct
    30001 gcgcaagctg cgccacccca cgcgcatcag caagctgggc ggtttctacg gcggtaaatg
    30061 atggcggcaa cacaggcttg accctccccg tccgcagagg caggctggcc gcgccatgaa
    30121 cacagacccc cagcccgacc cccaggagca gacggccgtc aaggaacaaa acgacaccct
    30181 tgtcgaggat gacttcgccg gagaggtgct cggcgagcgc caggctgaag cctgcaatct
    30241 cgaggaaggc tgcgagcgct gccagtaaag cgccggcctc cccaatcgtg ccgtccgggc
    30301 tgaaagagtg cccgcgcgcg gattgttttc gttgcctcaa aacgccgccg cccgtaatgc
    30361 aacagccatc ttttccaccc tagacgccac atgtccgcca ttccccacct tcccgccctg
    30421 cgcaaaggcc gcccctacga gtccctcgac aaagtccagg tcaaagacca caaaaccggc
    30481 gaagtctgcg ccgaggtcag ccaggtgaac gcgggcatcg ttcgcaaaga cctcggcaag
    30541 atcagcgagg cacgcaaggc attgaaaaaa ttcaccgtcg cccagctcat tgaaatcacc
    30601 gccaaagccg gcgagtcgtt cctcaacggc acccttcctc tcggcgacaa aggccacacc
    30661 cagagccctg aggaatacat cgccaccctc tcccgcacca gcggcctgcc gcacatcatg
    30721 gtgaagcgga acatggccaa gatccattac gccatgacgc acatgagcgt catcctcaac
    30781 ggcctctccc gcggcctcga catgagcgtc atagaccgcg gcttcggtga gcagtccggc
    30841 tgccccgtgg cctacttccc gacgacccat tccctcggtc tcgtcatgcc gagcaactcg
    30901 cccgccgtca actccctctg gctcccttcc atcgccctca aaatcccggt tgtcatcaaa
    30961 ccgggccgcg aagagccctg gacacccttc cgcctcatcc aggctttcat cgccgcaggc
    31021 gcgccgcccg aggccttcgg cttctacccc accgaccacg agggcagcgg cgaggtcgtc
    31081 aaactctgcg ggcgcaacct cgtcttcggc gatgtcaaca tcgccaaaat gtacgagaat
    31141 aacccacgcg tgcaggtcca cggccccggc tggagcaaga tcatcattgg cgcggacaag
    31201 atcgaaaact ggcgggagta ccttgatgtg atcgtgggct ccatcagcga caacggcggc
    31261 cgctcctgca tcaacgccag cgccgtcatc gtgccgaaat acggcaaaga aatcgccgac
    31321 gccatcgccc agcgcctcgg ccccgtcgaa cccctgcccg ccgacgacga aaacgcccgg
    31381 ctctccggct tcgccaatcc caagatggcc gactacatag acgccgccat agacaacgac
    31441 ctcaaaatcc ccggcgccga ggacgccacc gccaaatacc gcaatggacc tcgcaaagtc
    31501 gaattccagg gcggcatcta cctgcggccc accatcatcc gctgcgactc ctgggagcac
    31561 ccgctcgcca gccgcgagtt cctctgcccc tacgccagcg tcgtcgaggt gccgcaaaac
    31621 gaggtcctct ccaagatcgg ctactcgctc attgtcaccg ccatcacgca ggacgaggcg
    31681 tttatcaatg atctgctcga gtgcggtgac attgaccgcc tcaacatcgg tccgatcagc
    31741 accatgaaga tcagctggga ccagccgcac gagggcaaca tgttcgagtt cctctacaag
    31801 cgccgctcca ttgattgcgc gtgagagttt aagaaagccg cgccgccatt ttcatagacc
    31861 gccgtggcct tgccaccact gtttgatcca taatgatcgt tccatccccc ttcgactatc
    31921 agccccgcac ccggctcgtt tttggcaatg gtacgctctc ccgtgttgga gaactggctg
    31981 gcgaacttcg aggaacgcgc gcgctggtgg tcagtgatcc agggatcgtc ggcgcgggat
    32041 atgtcacccg cgcagtcagc ctgctcgtgg aggccggcct ggaggctcgc gtctatggcg
    32101 acgtgaggga aaacccctcc actgaggatg tggaccgctg cgtggaagcc gcgcgggagt
    32161 ttcagaccga cctcatcatc gggctcggcg gcggcagcag catggacacc gccaagggct
    32221 gcaacttcat cctcaccaac ggcgggcgca tgcaggacta ctggggcacc ggcaaggcat
    32281 caaaacccat gctccctctc atcgccatcc ccaccaccgc cggcaccggc agcgagtgcc
    32341 agagctacgc gctcatttcc gacgccgaga cgcatgtgaa gatggcctgc ggcgacccca
    32401 aagccgccgc gaaaatcgcc atcctagatc ccgaactcac cgtctcccaa cccgcccgcg
    32461 tcaccgtctg cacaggcatt gacgcgctct ctcatgcctt ggagaccgcc gtcaccagca
    32521 agcgcagcgc cctctcctcc gtcttttcac gcgagggctt ccgcctttgc catgagggct
    32581 tcagccgcgt actgcgcgat cccgcggaca tcgaggcgcg cggcatgatg cttcttggcg
    32641 ccgcctatgc gggcaccgcc atcgagaact ccatgctcgg cgccgcccac tcctgcgcca
    32701 accccctcac cgcctatttc gacgtcgttc atggcgaggc cgtcggcgtc atgctgccgc
    32761 acgtcatccg cttcaactcc gccctgcccg aaatagccgc catctacgcc ggctatttcg
    32821 acggcgacct ggctgaccgg gtcaccgaat tgctctggga ggcaaagatg ccgcgcaaaa
    32881 tcagcgacta tggagccacc gaagcagacc tcccgaaact cgccagcctc gcggccaaac
    32941 aatggaccgc gcagttcaat ccgcgaccgg tggctgaggc ggacttcgtc gagctttaca
    33001 gggctgcgct ttgatcatcc gtgtccgctg cccaccctgc gcagccagac aatgcgcgtg
    33061 gagccatagt cgcgctcgcg catcagctcc cagccctcgg gggcgggcgg ggcggatctg
    33121 cgggtggcga ggctttcgag caccacgctg gctccggcgg gcgccagggc ggtgaagtcc
    33181 gcgctctcca gcagctttgc gcagaggtcc gcctccccag gcttgtgggc gtagggcggg
    33241 tcggcgaaga cgaggtcaaa gcacgcgctt tctctggcga gttggcccac cgttttgaag
    33301 acatccccct ggcgcaccgt ggctccggcg aggcgggtct tggccaggtt gcggcggatc
    33361 acctcgcacg cgccgcggtc ctgatccacc agcagcgcgc tctccgagcc gcggctcagg
    33421 cattccaagc ccagcgcccc cgagccggcg aagagatcga gaacccgcgc cccctccacc
    33481 agcggcccga gcatgttgaa gacggcctgc cgcacccggt cctgggtggg gcgggtgacg
    33541 gacttgggca catccagcgg gatgccgccc gcgctgccgg agatgagacg catggagaga
    33601 agggctcgaa gctcgaaatc cgaaattcga agggcggaca ggaacacgaa agcggcggtg
    33661 cttcaagggc accgccgcgg agtttcgatg tggagtttgt gtttctcagc tcaggcttcg
    33721 cggccctcac ttcgctgaaa caggcgctgt caccgcaccc gcaggcactt tgccggcggg
    33781 gacgatcttc cacacagcgc cgcgctcgct ggtgtagggg tccacggggc cgccgtagtt
    33841 ggcggtggcg tggctcatgt agatggcgcc gtccgggccg gtgccgctgc cggtggggcg
    33901 gatgccgtcg gccaggtcca gcagctcctg catctgccac gcgccgtcca cctgcttcat
    33961 gccccagatt ttgccgctgc cccagtcggc gcagaaataa atgccgtcca gctcggggta
    34021 gtctttgccg cgatagacgc cgagattgat gacgcagatg ccctggtcca cgtggctgta
    34081 ctcaccaatg ggcagcacgc ccacttcggg gtagttctcc gggtccacgg cggtctcgct
    34141 gccatccggg ttctttttca ggatcatcgg gaagggatgg ctgccgcaca tgaatttcca
    34201 cccgtagttc tcgccgccct tgctggacgc cggctgcatg ttgatctcct cccagtggtt
    34261 ctggccgatg tcggcgatgt aaagatcccc cgacttgctg tcaaagctga aggtccacgg
    34321 gttgcgcagg ccgtaggacc agatctccgg cttggccttc gggtggattt tcgaaaacgc
    34381 ctcctcgctc acgccaaaga gcgtcatctg ctggagcggc gtgatgaagg ggttgtcctt
    34441 cgggatgctg taggcgcggt cgggcgtgct gtggtccacg tcaatgcgca gcatcttgcc
    34501 cagccaggtg tgcaggtcct ggcccgcgtt gatcacatcc ccttcccagc cgccgtcgcc
    34561 ggcaccgatg taaagatagc cgtccggccc gaaggcgatc tggccgccgt ggtggttgca
    34621 gtaggggaag tcaatgtgca tgatcacccg cgcgctgtcc gggtcgagcc ggtcggcatt
    34681 gctcttcgac acggtgtatt cggtgatcag cgtggcgccg ttgaaccaca ggtccgcgta
    34741 ggccacatag accttgccgt tgtccttgaa cttcgggtgg aaggccatgc tgtacatgcc
    34801 cagctccagg aaggaggaga tggtcttgtc cttgatgtcg agaaagggac ggcgctgttt
    34861 gacaccgtcc ttcagcacct ggatcacacc ggggcgctcg atgatgaaga cgcggcccgt
    34921 gccgtcatcc ggcgcggcca cgctcacggg gtccacgagg tcgtccgcca ctttcaccag
    34981 ggcgatggcg gggttccccg gcagcttgcc gccgggctgg gcgggcttcg gctgcgcggc
    35041 ggaggcggtg ctggacgcgc cgagaagcgc ggcgaggacg gagagagaag acagtgtttt
    35101 catgtgaggt tggaggattt atgtaaaatc acctgaggct aaaaatccac ccctgcgaag
    35161 gcaagacgga aaaatgggga aaatgagaaa catgttctcc gcaggcaggc gtctagcgaa
    35221 agaggggctt aacgaaaaaa gccccggcga taaggccggg gcttcatagg agtgcgagaa
    35281 tggatcaagg ctgcttgttc acagcgttgg aggtgtggtt gcgcactttg actgtgaagc
    35341 ccgatagttg gaaatattgg gttgccaggc tgttaaccac acctgcacca ttcagataaa
    35401 cggtgaccga gacattgttg ccggcgctct tgtcggcatc tgtgtaaacg agagaaactg
    35461 gccggctgtc ccctttgagg ggcagttttc gtccgttaaa gatcatctct ttcagattac
    35521 tcgagatgga gaacgtgaca ctggccggag caacgggcgg caggccgctg ggcggcggtg
    35581 acggatgagg ctgttctgtc agcttagcgg agtaaatccc cgctgtctga ccggcgctgg
    35641 caacgaaggg atcaagctcc accggccccg gaagatcgaa gccgccggaa atcaaggtct
    35701 gcgttttcgg atcaagcggc ggaatcaaca aatggccgta agcgtatgga acgaggaggt
    35761 cgttcgccat gagttgctgg tagacgccgt tgatgaccac tttgcgcttg atggtttttg
    35821 tcgtgagttt tggcagtgtg ccggtggtga ctgtgtcctg aatgttcaag acaagtgtca
    35881 tcaaaccggt cttgggatct atcttgccag agaagacttt ggcccattgc tcggggtata
    35941 aaggtgacgg gctgcccgag agaccggcgg caacgcggaa gatgttcttc gcagtgatgc
    36001 ccaactggct gggaaggaac ttggcgtaag tggtggtgtt caaaccattg aagaaggcga
    36061 tgtcgagaac ttgatcgtcc cccatcccga ggatgccttg tagggtctcg cctttgccag
    36121 gcggcttcca ctgggtcaca cgagtttcaa attcgacatc aaaaccatcg cggtaagttt
    36181 tgtctttttg attttcagcc ttggaccacc gcgcccatcc atcgccttcg gcagagtgga
    36241 accaaccgtc tccacgcgct gtgagttgga accagaaggc gaagtagctg ccaatcgtct
    36301 tgtggacgag aatgtggaag cgatagatgc cgtcctgact tggtcgcgcg ctggcggtga
    36361 tcttggtgcc atcggccagg gtgcccttaa gcgcgagcac gccgtccggc ttgatcacgg
    36421 cggagccaat gcagacgcct ccgggcggtg gtgtggagcc gagatcgtct gcatcaggga
    36481 aggcaagggt gtaggtgccc atccacgggc agtcatctgt tttggccttg tagaaccgca
    36541 gctttgaggc cgtgcccgcc tccactgtga acgaaccagt gtagccgggt agctcggcgg
    36601 cgccggtcaa ctcgaaagtg ccgtcctttt tgagataaag attgatcaca aacgaaagcg
    36661 gcggcggacc ggccttgggt ttgacgacgt tgaagttctc caactcagcc cagtcgttca
    36721 cctcgtcgaa cgcggccttg ccggcggcct tgtaagtctt ttggttcagc atcgtgaagg
    36781 tggtgctgag cgcgcccttg ctggtgagcg cgatctcgac gcgcccggca ggatcgccgc
    36841 tggtggagtc cacgagcaga ccctggtaac tgcctgcgaa cggggtgaag acatcttggg
    36901 cgcggatgtc tcccatcgtg gccatgaagc cgaggacgag aacactttgg acaatgtgga
    36961 ggatggggtt tttcatgagc ggcccaagga tgcctcctcc aggcttgggc gcaacccggg
    37021 attcacgcac ggttcatggc gcctgcctgc caaatttgcg ctccagttca ccaagtgaca
    37081 tctgaatgag cgtgggccgc ccatgcgggc agcagtaagg catctcgcag gcgaacaaat
    37141 cctcaagcaa tgcctgctgc tctggaagcg agaggcggtc gtttgccttc acggaatgac
    37201 ggcagacggt ggtggcgatc atgtcctcgc cgagacgcag gctggacgtg cgggagctga
    37261 ggcttttcaa ctcctcgacc acgcggtcca gccatgcggc tggatcgtca gccctcacaa
    37321 aggagggcag ggcctcaatt ttaaaaatgt tcggaccgaa gggctcggcc tcaatgccca
    37381 ggcgggaaag gggttccaga ttgcgcgcga gcacgtcggc atcgcgcggg ctgagttgca
    37441 gcgtgagcgg catgagcagg cgctgggaag gcacgccgcc ctgctccatc gcgcggcgca
    37501 ttttttcaaa gttcacccgc tcatgcgcgg catgctggtc catgagcacg aggccctcgg
    37561 cgctttccat gaggatgtaa agtttgtgca gaacaccgat gatgcgaaac gcaggaaccc
    37621 cacgagcttt tttttcaaca gcggcctccg ggttgggaac tggcggcggc gcttccggag
    37681 tggtaaccac cggcgaaggg gcgatgattt tttctgccga gttggcgtct ggaccggtga
    37741 acatgcggct ggtcatgggc ggccttgccg gctctgcctg gacaacccgc ggcgcgtccc
    37801 cggtcgcgac ccgcgccgtc tgcaaaggca gcgcagtctg cacctgcgcc ggagcaaatg
    37861 cctgggggcg cgggctgagc gtgctgtgcc ctccgggaag acggcccgcg ctctccagag
    37921 cgcggcccac ggcgcgcgcc acggcttcac gcacggcgta gccgtcgtgg aaacgcgcct
    37981 cttttttggc gggatgcaca ttgatgtcga aagtttccgg cggcatccgc agaaacaaaa
    38041 aagtcaccgg atgctgccct ttcatgagcg cggtgtggta gccctccttg agtccgtagt
    38101 tgatgatggc gctttcgatc ggcctgccgt tgaggaacgt gagctgctgc tggcggttcg
    38161 agcggctcag gcccggccct ccgatgtggc cctcgatccg gattccatga tgctccacag
    38221 gctccacttc gatcaaacgc gacgcgaggt cctctccgac cagcccgcgg atgcgctcca
    38281 gcaggtcccg ggtggcaggc aaatgaaaag ccacctcatc gtcccggatc aaggtgaacg
    38341 cggtctccgg atgcgcgatg gcgtgcagcc tgaactgctg ctcgacgtgc gcgtactccg
    38401 tggcctcggt gcgcaggaat ttgcgccgcg cgggcacgtt gaaaaaaagc gagcgcacct
    38461 cgatcacggt cccatgcgcc ccgccgtggt ctttcaccgc ggcgagtctg ccgccgttga
    38521 tctcgatctc ggttcccgcc agcgcctcgc gctcgcgcgt ggccagccgg aaacgcgaga
    38581 cgctggcgat gctcggcaat gcctccccgc gaaaaccgaa ggttcccacc gcggcgagat
    38641 cctccttggt gcggatcttg ctggtcgcat gcctttccat gcacagcatc gcgtcctcac
    38701 gatccatgcc gccgccgtca tccgtcacac ggatcaaggc cgagccgccg cgccgtactt
    38761 ccacagtgat gtgcccggcg cccgcgtcca gactgttctc cacaagctcg cgaaccaccg
    38821 cggcaggccg ctccacaacc tcgcccgccg ccacctggct ggcgagcgcg tctggaagaa
    38881 tgcggatttt tgacatattg gctgcaagct gcgcggttcg tcgggagtaa ctgttgcatc
    38941 gtcatcaccg atgtggaaag caccaacgtc attccgtttc cacctttcat gatccaactg
    39001 acagacagcg ctcttcaaca tctccggcat ttgctggacg agaagggcgc gcctgtggga
    39061 agcgggctgc gcattgcggt ggaaagaggt ggttgcgcgg gtcttcaata caccatgcgc
    39121 atctcccagc cacaggatgg cgacgaggtg gactccaagc agggagtgcc gctcatcgtg
    39181 gatcgcgaaa gcctcgccta tctggagggc tgccagattg attatctgga gtccctccat
    39241 gattccggct tcaagatcga aaatcccaat gccgcccgaa gctgcggctg cgggacatct
    39301 tttgagccgc tcaaaggcag ctaaaccgcc caaatccaga cgattgccaa gtttttcaca
    39361 aaaaaatctt ttctttttga ttcgaataag taaaatttca cggaataaaa aaccttgggg
    39421 ttctaaattt ctataatttt ctcaattttg ccatgcaagt caaatttctc agcgacggaa
    39481 gcgagtaccg ctcttatggg ggcggcagca ccacacagaa aaaagaaggt ggaggcatct
    39541 tttggtgggc tgtgctcatt acacttttga tgggagcagc aactttttgc tggtttttca
    39601 gcatcatggt gttctcccag ccggagaagc ccttcaacta caagatcctg gccaaattca
    39661 aaaaagtcac cccgctgcgc ccctacaccc tctacaccgc acccgccggc aaggcgtttg
    39721 ggccgcgcga actgctgacc gagttttacc cctatagcct tgagcagctt caactgcgca
    39781 acgacctgct caagcgcacc tatttgaaaa actatcaaca cgagcagccc gtttatttgc
    39841 tcggctccta caaggtgctg gaagtgcgcc agctcactga gggagatgtg ttctcatccg
    39901 gctgggtttt gcatgcgcgc gcctctgagt ttgaggatgt ggacatggag tttgtcatgc
    39961 caggactttc actcgcggac gcaccctgcg ccgctggcga tgtgatcaaa ctcgacaaac
    40021 gcgcctacgc ctccgtcctc catctcgaac ggctgggaca agaccgtgtc tgcgccacag
    40081 tcgtgccgct ggcttaccag acagtcgtgt ttgcgaacgg aaaatcgcac tccctgactc
    40141 ctccggaact gctgaacatc gaatcagtgt ggccgctcac tcgcgaccca ggcgcggaaa
    40201 gcatcaagcc tgttgacggg gaaaaagctg gcatcgcggc cacccagcct tgatctcatc
    40261 atcagatcgg aatcgccgcc gacgatggcg gcacagtcga agcggttgag atgagcgtcg
    40321 tggagcagtt ggacggcgag attcacgtca taaaacgatg gcacctgatc ccaacgaacc
    40381 agcattccca tcgtccgcgc catactctcc ggaagcaaaa acagatccac gggaggaggc
    40441 ggtctcgtcc agcatgctga tctgggtttt catctggtgc gtgggggcgc tggtatctca
    40501 ggacatggca gggaaaagca actccgctca aaatacggat accgctgaca ttgccccgtc
    40561 gaggcaatcc accgcgctcg tggacctgaa ctccgcgacc cctgcggagc ttgagcggct
    40621 tcccggtatc ggccctgtgc ttgcgattcg gattgtggag catcgtccct accgctccat
    40681 cgaggaactg gcgcgcgtgc cgggcatcgg agaaaaaatc atcctccaac tcaagcccct
    40741 ggtgaaaacc gaagtctcca cgccagatta agccaaggct cacaaccgcc tgcccagccc
    40801 gcgctccagc ccctcgaaca cctccgccaa aaacggccgc gccttcaccc ccgcctcccg
    40861 ccacgccagc accaccccgc gctgataggc cagcgccgac tccaacgccc tgcggatcac
    40921 cggctctccg ccgcccaggt aaggcttggc aaactccggc aaaaaccccg catacctgcc
    40981 cgagcgcacc gccagcgcca cctgcgtgta ggaggagcac tccagcgccg cgttcagcct
    41041 gacgcccgcc gcgcgcgccc gctcctccag aaaacgccgg aaatgccccc ggccctccag
    41101 caccgcccag gaaaaagccc ccaagtccac cgcccgcagc gccgccgtgc cgcccgagag
    41161 cgccttcggc acaaaagccg cctgctcata ccgccaagcc cgcgcgcaca ccagtcccgg
    41221 ccccgcatcc tccgggcgga tcaaagccgc gtccaccacc ccctgatgca atacctccac
    41281 cagccgcgca ctttccagat ttttgaaggc aaacaccacc ggctcgccca gccccctccg
    41341 caccgcctcg caggccggaa acagcagcca ctggatcagc gtctcccccg ccccgatcac
    41401 cacccgccgc gcctcgcccc cggcgcgcga gcgcacatcc tccgccgcct gaaaaaacgt
    41461 gcgcgccgcc cgcgccaact cccgcccctc ctccgtcagg cgaaaaggct tcgactgccg
    41521 cgccagcagc gccaccccaa acaccgcctc cagttccgcg atctgccggc tcagcaaact
    41581 ctgccggttc gcatcccccc gcgccgcctg cccgatgctc cccgcctccc ccacctcgca
    41641 cagcgaggcg aggcgttcga gggacaaacc ggaaagactg gcgagttcac ggaacatgac
    41701 tggggagtca tgtagcatgg ggtcacgcca ctcgccaaca cgcaaatgcc ggagaataga
    41761 tatgacctca ccggagcagc agaaggggcg agtgatcctc cagttgcagc ttgcttcgac
    41821 ttcgtctctt gattccacca ccatgatcag ccagctcaca ctcgaaaact tcaaagcctt
    41881 cggcaacgca caagcggtgc ctgtgcgccc gttgacattg attttcggcc cgaacagcgg
    41941 cggcaaatca agtgtcttgc acagcttgaa gtgggcgaag cacgccgtga aatccaaatc
    42001 gctcacattt gaatcgcctg gcggcagaga tgatgccccg gattttggag gttttctcgg
    42061 ggctgttttt ggcaaagacc gccatcggcg aattcgcttc ggatttctat taccatccag
    42121 aaacgcacct gactaccgcg tcagcttcga actgggcctt tggacccgcg agcttaaaag
    42181 gctagttcgc aaagaaccgg agcctcctaa tgagatcaag gactgggtgg cagcccagaa
    42241 cgagtttatt gatgcagaaa ctgaggcatc tttgcgcaaa gaatacctgc ataatttcgt
    42301 cttcgatgaa gacggcaatc ctgtccgcga cgataatgga gaattgatcg atctgaaaca
    42361 tgacggctca gagcggatcg acttcgaagc actagccgaa aaaatccgtc aatattggga
    42421 tccgcgcctg gaagagctat ggaatacatg gcggagcgag aatgccaagc ctggcatcag
    42481 cgaaaaggtg ggccgctact gcgaacgctt ggagcactat catgattggc tcgacaaaca
    42541 agagacagac ccggtcgtct ttggatttga gatcgcaatt ggcgagaatg tgcttctgaa
    42601 agcggagagg aaaacttttg atggcttcgt ccaactgaca aaagtcagca gggcgctgtc
    42661 ggcagtctgg tcatacatgg atgaaggcag tgacagtgca gcttgggagg ccagcagttg
    42721 gagcacccct ccgccttggg tggtgacaca tccatcacag gtattggact tggccgattt
    42781 gtgggatgaa ggcatttggt ccggcgaaga tctgggagac tgtttgggga ttgaatttca
    42841 aggattgatc ccaagtctgg ccaaccaggc tgtcttttca ggacaatgcg acctagctat
    42901 ggaaaagcag gatctcgaat gtttggaaaa actcatccgt gactgccgag tccgccttga
    42961 agggacaatg acaaaaatga gctaccttgg gccactgcgc acacttccgc caagatcgct
    43021 tctgctgttc gaaaaagatg aaagaagcgg ggctgtggct gacgcttggt tgcggcttcg
    43081 tgatgactct aaactcaggg acgaggtgaa ccgctggatt ggaagcggcc atcttgattt
    43141 gggttatcgt tatgaatcta aagtctattg tgatacctat tcactacttg aaacgatgcg
    43201 caacgaggag cgcgcgctaa gtattcatcg cgaatggaat ttagcagacg aatcctatga
    43261 gtctggatat aaagttaaag atctacatga atcaaaaaca ccagatttta gcgacaatga
    43321 gtatgaagct gtgaatttct ggaatgaccg gatttccgaa aaacttcatg agttggttct
    43381 tcgtgacttg aagaacaatg tcgcggtcag taaccgagat gttggtgtgg ggatcagcca
    43441 gttgataccc gtcattgtcc acgcattggc ggaaaaagga gctgcgattg ccgtggagca
    43501 acctgaactg caccttcatc ccgcccttca agccaagctt ggagatctgt tcatcgagtc
    43561 cgccctcggc cgcggcaaca ccttcgtcct cgaaacccac agcgagcatt tgattctgcg
    43621 actgttgaga aggattcgag aaaccacgga aggagagctt cccgatggca aattcgcgct
    43681 cactccagac caaatctgcg tgctttatgt ggaacccggc gagcaaggca gcaagatctt
    43741 ggagttgccc gtcacaccgg acggtgactt cagtcgtcca tggccgcagg gtttttttga
    43801 agaacgcagc gctgaactct tctgactcaa agcatgatca aggagattgc cctcgaccct
    43861 tgcctctttg cccaatggca gcattacctc gctctgcggg atgtctttgg cgtggagaaa
    43921 ggccgcgtca tctctgcctt ctcaaaaaag tggaaaggtc tcgtcagaga ggaggtgaac
    43981 cgcctaattg ctgctgaatt gatcggcgcc aacaaaggta agagcatcct agcgtggttg
    44041 acagttccat ttggcaccac agaaacccgg atggtcacca gcgcagcggc gtatgatggg
    44101 aacaaaccct ggcacctcaa cgcggaggac aactggtcct catttgatgc catcctctcc
    44161 catcgagaag tcacctctga gaacgcagtt cttgcagatg atgaatacga atatctacgt
    44221 catccaaaac tcacccctga aactcaacgc acgatcgctc ggcaacgtgg cccgatagtg
    44281 gattgcgctt ggtccttgct gcgtgactcc aaggtggtga agttggtcga gccgcacttt
    44341 aacccgcata aacctcggtt tcttgatgtg ctggaagcga tgcttgaccg gcttcacacg
    44401 gaaggttcat ctgttcgcga gatcgaactg catgtgcgcc atcccgaaga caaggatcct
    44461 caaggcacca ctccacccct cttcactgtg cccgaattga ggagcaagct ttctcccctc
    44521 ctgcggtcag gctggagcct ccgggttcat ctctggagac gaggccgtga gaagatgcat
    44581 ccacgctacc tgctcaccga tcgtggcggc attcagattg accatggttg ggacgagggt
    44641 gagcatgaca cggaaacaac cccgatcctg cttttgaacc gtgctcgatg ggagcaagag
    44701 atgcagagat accagccttg cagccaggac ttcatgatga atcccgtcac ggatattgtg
    44761 catatcacat gaactttaat gatctcaccc gcccgtcatg ctctccgcct gggcgaggac
    44821 ttcggcgaag gggacgcggt ccagcaggcc gaagctgtcg tggcgggtga ggcgggcgcg
    44881 ggtggtggcg gggctggtga ggccgcacaa aaagcgggcg agctggcgcg gggtgcgcag
    44941 cgcagcgtgg cgctcgtcga gcagggcgcg gatgagggcc acgtcttcgg tggtgatctc
    45001 gggcgcagcg gcagcgggga tgtggcgcgg gctttcgagg tcggcgtcac cggtgcgctt
    45061 ttcctggcag ctcgtgcatt ggccgcagtc gctgggcaat tcctcgccga agtaacgcag
    45121 cagccagcgc acgaggcagt ggcggtggga ggcgtagtcg agcacttggg ccaggcgctc
    45181 cacctcgcgc gcctcacgcc gggtgaagag ctgctccatc tgccgggcga gcgcggcggg
    45241 gtcgcgccgc gcggcatcgg ggcagaggcg gtatttttgg cggctgccgc tggggcgcag
    45301 ctcgatctca ccggcctcct gcagccaggt gagggcggag aggatgcgct cgcgcggttc
    45361 ctggagttcg agcgcggctt cgtcggggtt cacagtggtc cagacgcggg cttttttgcc
    45421 ggtggcgaag aggcggcgca ggaagtcctg gcgcgcgggt ttgtgcccgg caagcacgcg
    45481 ctccaggggc tggagcaggc ggtattgata gctggcgtaa aagctgccga gcgggcgcag
    45541 gatgccgccg agctcgagat ttgtgagcac ggtctcgatg acgagcgggc ggatgtcggt
    45601 ggcgtgggag agttcgtaga gagagaagtc gaactcctcg ccctggcgca gcaggtggtc
    45661 caaaagctgg cgcagggcct ggggggtggg ggtgtcgccg aaggtgaagt tttccagcac
    45721 gatgcggtcg tcggcgcagg cgagcagctc gcagatggag ggctggccgt cgcgcccggc
    45781 gcggccggtt tcctgcatgt agttctccag ggtcttgggc aggttgtaat gatagacggc
    45841 gcggatgccg gccttgtcta tgcccatgcc gaaggcgatg gtggcgacga tgatgtccac
    45901 cgcgccgctc atgaaggcat cctgcgcctc gtggcggtgc tcgtcggcga ggccggcgtg
    45961 gtaggcgcgg gcgttgaggc cgcttttttg caaatgggtg gccacgtgct cggcggtctg
    46021 ctgaagcgtg acatagacga ctgccgggcc gccgcgttgg cgcaggcgag cggtgagtgt
    46081 gtcgagccgc tgccgcccgg cgctgggggt gatgtgatac tgcaagttag gccgcagaaa
    46141 ggaggtctgc acatggtccg ccccggcgat ggcgaaggcg gagcagatgt cacgcgcgac
    46201 ggcgggcgtg gcggtggcgg tgagggcgag cacggggtgg atgcccagct cgcgcgccat
    46261 gagtgccagg cggaggtatt ccgggcggaa attgtgcccc cactcggaga tgcagtgcgc
    46321 ctcgtccacg gccatcatgg cgatgcgcgc gcgcttcagc ctgacaacaa agttttcatt
    46381 catgagccgc tccggggcga tgtagagaag cttcaaagcg ccgctctgga ggtcgtcata
    46441 cacctggcgg gcctcgtcgg cggagaggga ggagtccagc cgcgcggcgg gaatgccgcg
    46501 ccgttgcagg gactccacct ggtctttcat gagggcgatg agcggcgaga tgacgagcgt
    46561 gacgccctcc agatgcaggg cggggagctg gtagcagagc gacttgcccg cgccggtggg
    46621 gaagagcgcc agcgcgctgc gcccctccag cagcgcgcgc atcacgcgct cctggccggg
    46681 gcggaagccg gcgaggccga agcggcaacg caaagtctca tggatatcca ctctcatgcc
    46741 cccgtgctct caaagactgc cgccgtaatc gctcggcatg cgccagtcgg cgcgggggga
    46801 gagggccacg gtgccgactt ttgggccgtc tggcatggag gagcgcttga actggttctg
    46861 gacgaagcgg cgcaggaaga cgccgagcca tttctcgatg acctccggcg tgtgtgaccc
    46921 ggcaaaggca atcccggcca gatagcgcag cttctcccga ccgcagccgt ggcgcgcgaa
    46981 atggtagagg aaaaaatcat gcagttcata cggacccacc acatcctccg tgcgctgccc
    47041 gatgccaccg tcggcggcgg cgggcagaag ttcgggtgaa atgggcgtgg caatgatgtc
    47101 gcgcaggatt tcccccgcct ccgccgcgta ctgcccggcg gcgcaccatt cgatgagata
    47161 cctcaccagg gtcttcggca cgccggcgtt gacatgatac atggacatgt ggtcgccatt
    47221 gaaagtacac cagccgagcg ccgcctccga aagatcgccc gtgccgacga cgaggccgcc
    47281 ggtttgattc gccacatcca tgagcacctg cgtgcgctcg cgcgcctggg cgttttcaaa
    47341 ggtgatgtca tgccggtccg gcgggtggcc gatgtccttg aagtgccgct ccaccgccgc
    47401 atggatgggt atcacgcgca gcggcactcc gagggccccg gcgagttttt ccgcgttgcc
    47461 gcgcgtgcgc tccgtggtgc ccgggccggg catggtgact gccagcgcgc actcacggct
    47521 catgcccgcg cgcggcaggg cgtccagcag caccagcagg gccagcgtgg agtccaggcc
    47581 gccggagagg ccgatcacag ccgcggcgct ttttgtctgg cgaaggcggc gcgcgagtcc
    47641 ggtggactgg atggcgaaaa tctcccggca cgcagcatcg cggtcggagc ccggcggcgg
    47701 gacgaagggc tgcgcggaga gcgggcgcag caggcgggaa ctggccgcac gacggccgtc
    47761 cagagtgcaa accaccctcc ggaagcagcg gcccgccgcc tcatcgcgga aggccgcgct
    47821 gcgctggcgc tcatgctcca ggcgctgcag gtccacatcg gccagcaccg cgcgcgtctc
    47881 gaaggaaaag cgctccgact ccgcgagcag cgcgccgttt tccgcgatga tgccgtggcc
    47941 ggaatacacc gtgtccgtcg aggactcgcc cgccccggcg ctggcgtaga ggtaggcggc
    48001 caggcagcgc gcgctttgct ggagcacgag attgcggcgg tagggcgcct tgcccagcag
    48061 ctcgttgctg gcggaggggt tcgccagcac ggtggcgccg gccagggcgg cgtggctgga
    48121 gggggggatc accgaccaga ggtcctcgca aatctccacc ccgagcacga ggccgggcag
    48181 gtcccgggct tcaaagagca ggtccgcgcc cgccggcacg ccctccacct ccgccactcc
    48241 cagcgtgtgc gcgggggaaa accagcggcg ctcataaaac tcgcccgaat tcggcaggaa
    48301 cgtcttcggc acgaaaccca gcacacgacc cgcccccgcc acggcggcca tgttgtaaag
    48361 ccgcccgtcc accgccagcg gcagccccag caccgtcagc atgccggagt agctgctttc
    48421 ctcggccagt tggcgcagcg ccgccagcgc ctgcttttgc agggtgggga gataaaaaag
    48481 gtccgcgcac gagtagcccg tcaggcacag ttcggggaaa acggccaccc ggcagccctc
    48541 cccggacagg cgcttcatct ccgcgcggat gaccccggcg ttgaactccg catcccccac
    48601 ccgcagttcg ggcgacacca cggccgcgcg gacaaagccg tgcttctcgt gctcgggcag
    48661 gttctgggtt gggtcggaca tcggcgcacc ctagcacgcg cgggcggggc ttggggagga
    48721 aaaaacgggc gggcgcgaaa gttcaaggcc agcagtccgc ggccccggcc cgccgggcat
    48781 gacaccaggc gggcaaatcg tcacaaaaag atgctggtca atcccgcaaa gaccgtacta
    48841 gctgcccctt tgccggacaa tttcatgatc ctgacctttc tcaccatcct cggcagcctc
    48901 ggcctcttcc tcttcggcat gaagatcatg agcgaggcgc tccagaagct ctccggcgaa
    48961 aagctgcgcg gcatgatgcg cgtgatgacc acgaaccgct tcaccggcct gctctccggc
    49021 ttcctggtca cctgcctcgt gcagtcctcc tccgccacca cggtgatgat cgtcagcttc
    49081 gtcaatgccg gcctgctcac cctcttcgag tccatcggca tgatcatggg ggcgaacctc
    49141 ggcaccacca ccaccttctg gatcatctcc ttcctcggct tcaaattcag cctcagcagc
    49201 atcgccctgc ccatcatcgg cgtcggcttc cccatgatgt tcatgcgcaa ctccagggtg
    49261 cgcgacacgg gcgagttcct gctcggattc ggcctgctct ttttcggcct gctcatgctc
    49321 aaggactccg tgccgaacat ccaggacaac cccgagttcc tcaaattcgt ccagggcttc
    49381 accggcaagg gcatcttctc cgtcctgttc ttcttcgcct tcggcaccat cctcaccatc
    49441 atcgtccagt cctcctccgt ggccggcgcc atcacgctca ccatggccta caagggctgg
    49501 attgatttcc cctcctccgc cgccatcatc ctgggcgaaa acgtcggcac caccatcacc
    49561 gccaacatcg ccgccctcac cggcaacatc accgccaagc gcgccgcctt cgcccacttc
    49621 atcttcaatg tcatcggcgt catctgggcc atcctgctct tcgccccctt caccgccttc
    49681 atcagcgcca tcgtgcccgg ggacgccggc cagccggaag ccatgccgct ccacctcgcc
    49741 gccttccact ccggcttcaa cctcatcaac atcctcctca tgatcggctt ggtgcccctg
    49801 ctggtgaggc tggtggaaaa gaccatccgc agccgcgccc cgggcgtcgc ctcccatgtc
    49861 gagtacaagc gcgcctccct gccgcagacc gccgagctga acctcgccga ggccgagcgc
    49921 gaggtgcagt ccctcgccac ccacacccgc acgctcgtgt ccggcttcct ggatgtcttc
    49981 ggcaggcccg aggccgagat cagcggccgc atcaccgagc tgcgccaggt ggaggagctc
    50041 agcgacgaga tcgccctcga tgtcacccag tacctcgtgc gctgctcctc cgccgggttg
    50101 agcgacagct ccatggggcg cgtctccgtg ctcctgcgcg tcatcgccga gctggaggac
    50161 atgtgcgact gcggcaaccg cctcgtccag ctcgccagcc gccgcacccg caaaggccac
    50221 gagctgccgc ccgagaccct cggccagctc cgcgacatgg gcgggcacct tttcaccttc
    50281 atagacttcc tttccacctg cctcacccgg cccggccagc ctggcgatat ggagactgct
    50341 caagagatgg agcgccagat caacgcctgc cgcaaacgcc tgcgcaaaga gtccatgacc
    50401 cgcatgctcg cctccggcga gaccatcaag gccgaggtcc tctatataga cgtgctcaac
    50461 aacatgcagc gcatctccaa gcacgccatg aacatcctcc aggccctgcg ccaggagggc
    50521 tgaggcatcc ccgccggggg gagcgggcgg caggccgcct cactcccccg ccgccgccag
    50581 cgccgcgtcc actttgcggg acatgtcgcc ggggctgccg gtgcggcggg agatggcgcg
    50641 gtaggccagc gggacgacga tgagcgtgag cacggcggag acggccacgc cgaacatgat
    50701 gacgacgccc accaccagcc gcgtctccgc gccggggccg ccgccgagga aaagcggcac
    50761 ggagcccatg acggtcgtca gcgtggtcat gagcacgggg cgcaggcgcg tgctggaggc
    50821 ctcgatgacg gcgtcgtcgt aggccatgcc ctcgtcgcgg agctggttga tgaactcgac
    50881 gatcaggatg ccgttcttgg ccgccaggcc cacgagcatg atcatgccca cctggctgta
    50941 aaggctctgg ttctgcccca tgacgagcag gccgatgagc gcgccgccca cggcgattgg
    51001 caccgtcagc atgatggtga agggatgcac gaagctctcg aactgcgccg ccagcaccag
    51061 gaagaccacg aggatggaca ggccgaacac aaacagcacg ctgccgctgg tctcgcgcag
    51121 cttcagcgcg tcccccttgt agccgatggt ggcgtcggcg ggcaggttct cgcgcaccag
    51181 ggattccatg aaggccaccg cctccgagag gctgtaaccc ggcgccagcc cggcatcgaa
    51241 ggtgatggcc ctcatgcggt tgtaacggtt cagcgcgccc gagtcggcga actcgcgcag
    51301 gctcaccagg ttggccagcg ggatgagccg cccggaggtc tccgagcgca catagatgcc
    51361 ctgcaggtcg ccgggcagcc gcttgccctc gtagtcgccc tccaggatca cgtcatactc
    51421 ctcgccggcg tccacatagg tcgtcacgcg gcggaagccg agcaggctct ccaaagtgcg
    51481 cccgatgttg gcgatggaca cgcccaggtc ggcggcgcgc acgcggtcaa tggccacctc
    51541 caactgcggc ttggtctcct tgtagtcgta gtccagcccg accaggccgg ggttggcggc
    51601 ggctttttcc aggatgatgt cccgccagcg cgccaggtcc tcataggtgg ggccgccgat
    51661 gacgaactgc agcggcttgg tcagcccccg gctcaggccc tggcgcatga tgatgaaatt
    51721 ctgcaccccg cgcatgtcgg cgagcttcgc ccgcacctcg tccatcacca cccaggcgga
    51781 gcggcgcttg ccgtggtcgc cgaggttcac gatggtgatg acctcgttga agtcatagtt
    51841 gctggtgaag gagcgcggga tgcgcaccag cagccgctgc gcctcgccgc tgtccaccag
    51901 gtacatcagg cgcctctcca gctcggcgaa gatctgcatg ctgtagccgt acgacgcgcc
    51961 ctgcggcgcg gaggagacga tgaaaaagga gccgcggtcc tccttcggcg tgaactccga
    52021 gggcagcgcg cgcagcagcc agagcgtcag cgccgcgaag cccgccacca cggcgaaggc
    52081 cggcagcggg taggcc
//
LOCUS       DAOMYJ010000005        50732 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Prosthecobacter sp. isolate AMR_MDS_5629
            NODE_264_length_50732_cov_22.359473, whole genome shotgun sequence.
ACCESSION   DAOMYJ010000005 DAOMYJ010000000
VERSION     DAOMYJ010000005.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867850
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prosthecobacter sp. (wastewater metagenome)
  ORGANISM  Prosthecobacter sp.
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Verrucomicrobiaceae; Prosthecobacter.
REFERENCE   1  (bases 1 to 50732)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 50732)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.30
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:34:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,742
            CDSs (total)                      :: 4,684
            Genes (coding)                    :: 4,676
            CDSs (with protein)               :: 4,676
            Genes (RNA)                       :: 58
            tRNAs                             :: 55
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..50732
                     /organism="Prosthecobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_264_length_50732_cov_22.359473"
                     /isolate="AMR_MDS_5629"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1965333"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..221
                     /locus_tag="PLP58_00995"
     CDS             <1..221
                     /locus_tag="PLP58_00995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ07126.1"
                     /translation="PAPAKKEEPKKDEAKKTEPKKGAAKKEEPKPAATPPASKPANTP
                     PTPKPADAPPAPKPAAKPTDAPPAPKAN"
     gene            complement(224..1018)
                     /locus_tag="PLP58_01000"
     CDS             complement(224..1018)
                     /locus_tag="PLP58_01000"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR00121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004077 - biotin-[acetyl-CoA-carboxylase]
                     ligase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HRJ07127.1"
                     /translation="MPVLDAAVLQDACERDSLPWRVSVTAETGSTSDDLRAVALRGEP
                     PGAVLFAESQTAGRGRRENRWITPPGRDLMLSLLLRPAAPPVLWPRVTTLAALAVCQA
                     IERVLPLRPVIKWPNDIFLGRRKVSGLLAETLNGPEGLMLVLGIGLNVNTRVFPPGIA
                     SEATSLALELGDEVRMELDRHELALALLSALHSHLGRLDSGFSVALREARSRSLLLGR
                     QIRAWQHGREVVGRALDLDEEGRLKLELPDGSLSLLDSAENVRQVY"
     gene            complement(1025..1942)
                     /locus_tag="PLP58_01005"
     CDS             complement(1025..1942)
                     /locus_tag="PLP58_01005"
                     /inference="COORDINATES: protein motif:HMM:TIGR01297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HRJ07128.1"
                     /translation="MMKHAQAQAAARRAMRWSLSASFLMLALKLGAWALTGSAAILGD
                     AAESVVHLAAVFFASFSLALSQRPPDANHPYGHSKVAFISAGVEGALILAAAVFIAFE
                     SVRRWVSGLGVVNLDQGMVLTGVAILVNLALGLFLMRAGRRLDVFILRANGRHVLTDA
                     WTSLGALAGLAMTVLTGSLWWDPLFGLLIAANILFSGWGLVRESVTGLMDEADPAVSR
                     QLEQALAHETSARGVSFHALRHRNSGHLHHVDVHLLFPDGILLRDAHRRATEIERAVI
                     AAMPVKMQIITHLETCGDHDKVHAGDPDA"
     gene            complement(1997..2713)
                     /gene="infC"
                     /locus_tag="PLP58_01010"
     CDS             complement(1997..2713)
                     /gene="infC"
                     /locus_tag="PLP58_01010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012420765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HRJ07129.1"
                     /translation="MGAGFGPPRGNRNNNRYADQTRVNERIRAPKVRVVDGATNQQYG
                     VLPTGQALRMARERGLDLVEVAANLEVPVCKIIDYGKWKYIQEKHKKESHKHHKGGKV
                     KELKFRIGIDPHDYQIKIIHAEDFLAEGHKVRIQLQFRGRQMAHQELGHELAAKIKTD
                     LQTMGHADQEPKQAGRNINMQISPLPERQRVRKFKTHLKGVTEHADIKHQEGHDPRED
                     RHDEEDHHDDDHHEDGQKQA"
     gene            complement(3050..3463)
                     /locus_tag="PLP58_01015"
     CDS             complement(3050..3463)
                     /locus_tag="PLP58_01015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ07130.1"
                     /translation="MDYMCNPNWQGAFQKNFTDREKSIAGWPGPPKKWADHAYPTIEA
                     REDIKFFDTDCGRVVNNIACLRMIETTRAWLAPEASIHSSPFPQNPWVTRTLPEFVLP
                     RAKNPTIKFVVVSGGLFRPEAAMKTSASTHDFPDA"
     gene            3490..5427
                     /locus_tag="PLP58_01020"
     CDS             3490..5427
                     /locus_tag="PLP58_01020"
                     /inference="COORDINATES: protein motif:HMM:NF019223.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1549 and DUF1553 domain-containing protein"
                     /protein_id="HRJ07131.1"
                     /translation="MNFLSSPHCAILISTVLLFGVSGHAAEKYDNPTPWSYQRLQRPA
                     IPATTDTAWPKDDLDRFILARLEKENLRPIGDAPRAILIRRASFDLRGLPPSQSEVDA
                     FLRDPSSDDAAFAKVVDAFLQSERFGERWARHWLDVVRYADSVGRVWNAPFLYAARYR
                     DWVIDSFNDDKPYNRFVAEQIAGDLLPAKTVLQRRDQIVGTGMLALGSMNLQEGDYEQ
                     HLLDQVDDQIDVVSRAFMGITVACARCHDHKTEPVTTRDYYALAGIFYSSRTLSGTPN
                     RNDGVAGGYVSPEALVDLPTELDEAVGPPQKLPAGLHSMDDIRELGNPKQTIRYDHDP
                     HFCMGVIDAEIKDCEIAIGGDPHDRDTAPNRGELKLPALPPMPKVPAQSSGRLEFAQW
                     LTQPTHPLTSRVMVNRLWQHLFGEGLVRTPDDFGITGADPTHPELLDHLAIRFVTNGW
                     SVKKMIREMMLSHTYRLATTGNPEHPDAGNKLRWRQNPKRLELEPLRDTLLQLAGQLT
                     FDRPEGIQVAGTGGKGRHGITRGLLGIEEPYRTIYLPVVRDNIPELFSTFDFPGPTQI
                     KGQRDVTTVAPQALFFMNNPFVEQLSAQIAEKAGKDTQAIYKLILGRDPTSEEITGAR
                     DLDDQTLVQALLGTAEFRYVF"
     gene            5767..7116
                     /locus_tag="PLP58_01025"
     CDS             5767..7116
                     /locus_tag="PLP58_01025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009962498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1501 domain-containing protein"
                     /protein_id="HRJ07132.1"
                     /translation="MTTRRSILQSTSAGFGWLAFNALHQQWANAAAPPKTINPLAPKA
                     PHFAAKAKRVIFMFMQGGPSHLDTFDYKPELVKAHEQKVAKYMGTAFDFKPRGKSGLM
                     ISDAFPELSKHADDLCLLNGMQTRTNAHQMATVALHIGSETFVRPSMGAWIVYGLGTE
                     AEYLPGFVTINPVADTGGAMNYGSAFLPASFQGTRLSTTGGGVPDLSNGRLSDAQQRK
                     QIEFIQKANRRLLEGDPGNPELEGIIQSYELAYKMQTSVPDVLDLDKEPAHIRELYGL
                     DNGDTERFGTQCLMARRLAEKGVRFIQLTSTGWDHHQQLREGITRQATSIDKPIAGLI
                     ADLKQRDMLKDTLILWGGEFGRGSSYDNEPYNGRGHNGRGYTMWMAGGGVKGGFAYGS
                     TNAMGDTAETGIITTHDLHATVLHLMGINHERLTYRYSGRNFRLTDVHGEVAKDILA"
     gene            7249..7605
                     /locus_tag="PLP58_01030"
     CDS             7249..7605
                     /locus_tag="PLP58_01030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ07133.1"
                     /translation="MKSILAILALAAFSSLSAAEPLNTICPVSGKPANPSITSNYSKT
                     VAVCCDRCVSQFNATPKAYLRNIVTANGVQCPLSKKKADASKKVTYSRQVAFADAASK
                     AAFDAAPDKHIIEVRQ"
     gene            complement(7672..8259)
                     /gene="raiA"
                     /locus_tag="PLP58_01035"
     CDS             complement(7672..8259)
                     /gene="raiA"
                     /locus_tag="PLP58_01035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018971112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_component: GO:0022626 - cytosolic ribosome [Evidence
                     IEA];
                     GO_function: GO:0045182 - translation regulator activity
                     [Evidence IEA];
                     GO_process: GO:0009386 - translational attenuation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HRJ07134.1"
                     /translation="MQKHNVNLPVIVTGRHVEITEAIRDYAHKKIEGLHLDYPRIIEA
                     KVILDIEHHRQIAEIILFCANHIVIEVKSTTEDIYASIDESIAKIARRMRKFKTRLLK
                     SHRPRKEGSIRHLEQHVFHADDLHSEEEHVEAAYIHNEHYKVRPLYPDEAIMDLEISD
                     RPFVVFHNQKTHRLAIMFRRKDGEYGLIEPRDEAA"
     gene            complement(8305..9024)
                     /gene="lptB"
                     /locus_tag="PLP58_01040"
     CDS             complement(8305..9024)
                     /gene="lptB"
                     /locus_tag="PLP58_01040"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009961167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter ATP-binding protein"
                     /protein_id="HRJ07135.1"
                     /translation="MLLHTEDLKKVYDGRAVVNGVNIEVNAGEIVGLLGPNGAGKTTT
                     FYMIVGLVRPNGGRVVFDGSDATQLPMYKRARLGMGYLPQEESIFRRLTVRENIIAVM
                     ETQNFSRQEREDQCQQLMEKFGIDHVADNLALTLSGGEKRRLTIARSLVTEPKLLMLD
                     EPFSGVDPIAVSEIQDIIRMLRQAGLAILITDHNVRETLNIVDRAYLIYEGQVRRHGS
                     KDFLVNDPEARRLYLGEGFTM"
     gene            complement(9107..10066)
                     /locus_tag="PLP58_01045"
     CDS             complement(9107..10066)
                     /locus_tag="PLP58_01045"
                     /inference="COORDINATES: protein motif:HMM:NF015901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LptA/OstA family protein"
                     /protein_id="HRJ07136.1"
                     /translation="MKNLHHLLLTAMLMAVDAHGQDAPAVKKELRDAAQKLQQAREDG
                     ALEKAGDAARQMSEQIPAGVAEAAKSALESSELKKQAVDAANSLLPEARKRMDSGSSG
                     ESVGEKGGAAKQQPSGPQPQTLPPLDHAASKPRVPTVVIEADSSVFDLKQAVFVYTGR
                     VRARHPQFYIECDELVVEMENEEDKEKDKSDTQEEKAGSQKKKPGPDDGATQEKKTGN
                     AGVKKATATGSTVTIEKLTEEGETQIGKCRKAVYEAGTGEIIMSNYPQVQRGNILHLA
                     TQPDTLMIFDPNGKLRTTGRPRTIILNDEDKSTDTREGGNSGR"
     gene            complement(10068..10556)
                     /locus_tag="PLP58_01050"
     CDS             complement(10068..10556)
                     /locus_tag="PLP58_01050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ07137.1"
                     /translation="MRLAGACIGAGYFLAVFCAGSQEAESSMSAFGKWIPQGFVNRNV
                     VIPSFNDGKLGSRLTAATLVRLDDDRLEAGRTVIEIYGDTPPQDTRVEMPGAIFHMTA
                     NVVRSGERSRVIRSDFEAEGDSMVFDATTSIGAMKGRVKTFIFDTGPQTGQSKQSQPG
                     AD"
     gene            complement(10553..11389)
                     /gene="kdsA"
                     /locus_tag="PLP58_01055"
     CDS             complement(10553..11389)
                     /gene="kdsA"
                     /locus_tag="PLP58_01055"
                     /EC_number="2.5.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009961163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008676 - 3-deoxy-8-phosphooctulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-8-phosphooctulonate synthase"
                     /protein_id="HRJ07138.1"
                     /translation="MLSLPVVKVGSVLVDGTEPLFILGPCVIESEEFIWPVAERLSAL
                     ASSLRLRWVFKASYDKANRSSGASFRGMGARAGCQLLARIGRKLSVPVTTDVHSPGEA
                     AIAAEHVDLLQIPAFLSRQTDLIMAAGETGRAVNVKKGQFMAPWDVRNIADKLAAAGC
                     ENFLFTERGTTFGYQNLVADMRSPYWIRELGFRVIFDATHSVQRPGGLGNATGGDGKL
                     APVLARAAVAAGCDGVFMETHPDPEKALSDGPNQVPLDELAPVLRTLTLIHEIVKQGG
                     AP"
     gene            complement(11524..29121)
                     /locus_tag="PLP58_01060"
     CDS             complement(11524..29121)
                     /locus_tag="PLP58_01060"
                     /inference="COORDINATES: protein motif:HMM:NF025295.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="immunoglobulin domain-containing protein"
                     /protein_id="HRJ07139.1"
                     /translation="MPVTGTGILPGSYIVQILSGDQILINQSVTGNNSNVNLTFDDTT
                     VIHDPVTGADNFTKNGSDKVVLVGTNTTTGAFTINDGIVQVGGVNFSGRTVIHDVLSN
                     SGSLVFGATGAPTLNFASNVLNTAPFERVGSLSGGSNAATILLTGGTSNTVMAFGGNN
                     ATTSFTGSIIGGVASGLLKEGNGIFTWINNNDAAFNGTVMVDSGGMIVGGSAGLGNGR
                     FVGEGLGSNIVMSNKNGVSLTLASGGNEVVGFLTGGGFGTTRLFPNGSTGALLGNYLN
                     QAGGELILANTNLILDNNTPNQVYSYGGIISGTGNLTKVGNNTMEVLGTSTYAGSTTI
                     EAFAVNNTHSAIRLGAYGIHSGVGVNASAGFGSLSSTTHLMLSAGNVGSGRDVTFDLN
                     GGSQTLGTVNSANVAGVKTVRLQGGALTIHTTAGNSGFYNGIFSGRGTINVTSTTGAN
                     GWELRATDLANNDAIQRGALNVLGGRVSLNDSRGALGDAVHVSVGAAGTLSVLDNETV
                     GSLSGAGNVEITSGRTLVLASGPAGSVFDHAWSGNISGFGALTLATGGSLMVNSNQSY
                     LGATRVSSGAALYLDYESGATNLIPGTLNLNGGSLYLSGGAAPEVISSGTTISAGASL
                     IATQTGEADALLNLGNLIRNSTAGGVLNIRGGSATTTSTGQVGLGGILGGYATHRSVV
                     AGTPAFSWAVPGAGGAAISGLADGSYSSSFGAGLNTDVTSLFTDVKVSGATGSLRFNT
                     FGAQTLTIGGASDAQQTIVESGGIMVTPAVGAFDITITDQNRGNEELFGGGAFGVERE
                     LIVHQHNTRGTLTINATIGDNGGPSRLTKTGQGTLILTKLNTYSGQTAIYGGVLQLNT
                     GGILGGASQPLINHGYLVINRSSITLPQIIEGTGILRQAGAGITTLNSDNTYTGPTQV
                     LAGTLEISNAGNALGSTAGLTSVGSGATLSFSNVVVPETIVLKGGSTLRTSPSAGSST
                     LNGSLILGGNSTITLGGASSPLRLNGRVFAMQGSQLTVNGTGRLLLGNAANHFNGISL
                     GAGTQLLLGADPLLDNNPLTIAPAVAGAAGRAPIVNNGTVITNTNNGHMVLGNAISGS
                     GNFIQVRNTLYLTADNTYTGTTLAGGNFGFIGGGGLVGVANVNAEMRVGTDTYTGSVG
                     TGAVTLQSSSGGNTNMRYHLARNVILANTFNINPFNDGSNARNSTLLRQGTGTTIFSG
                     IINAGSHTAGTQAQRAVLQTEGGGRMIIRGELNNGPNNRINIINNGFFAFEGTNNYEL
                     WGVISGNNALVFNTTGTVTVQPARDQTNALVQSMTSNSTLYLSRGTMVINSDFDDSIN
                     NDMDMYVQRGATLQMNFNETIGSLLAQKGSLTVLGNGVTLRVDDNITRGNFGSITGGG
                     EMHFDAVGGTAWHGMFGVSDYVGNVTIGSGTQITTLRVNSVADRFLPSALGAGEAINF
                     GLAGSSLDTRLEYTGTGHSTDRDFNLSGASGTVRIAGNGRGGLVLNGNITITADGAKT
                     LMLHGQNTQNVINGALQRNGRLLSISVGPNVGDNDVYGAGKWTLTNASSDFNGDISVD
                     VGELEFAGDLGNGSTAFSVMGNLNVARTIDLATNNFNGRRFAFTGGGDNLGATGQPGS
                     NGTIIFNDPGAGTATLGSNITFTSSYSSTTNPGNPEIMNNGEKVIVINGNLTSGATGN
                     RSWILDGSNTGANTIAGSISNGTGNTVSVIKEGTGTWRLAGANTFTGAVTITRGTLEI
                     TGGSTIGDAAVVNLSNAGSDGSSAGGAALRVLSSETIGGLAGGLGTLVEISSGQTLRL
                     LSAASQTYNGVISGEGGLWRDGDATARIQTLTNLNTYEGATLISSASNIAHRIDVLFL
                     ADGGEASGIGASSNAASNLVLNTGTGIGGLRWIGTTNQTTDRLLTLGAGVGAGGIWAD
                     GQSFGDFAPSIKFTNSGAIAFLAPNTTQTLTLRGARIAENEFSPQITNNGSGATILNK
                     IEASTWIINGANTHTGGTTIGGGTLAVTHNNALGTGTVAINGGAGTGLQLRGGVTLAN
                     AVTNGTNLGGVGALTGVNRINGGVTLSGTNASFVNTVHDGASLRLAGTITGTVGTGTF
                     LKAGRGDLILSGASNSFTGVTTVAGGSLILDYTTSNNTKLAAGSALHLGWATLGSATG
                     FATGLGPSATVAAQTDLAPYSGGTLVLRGNAAAFTQVVASTLLNNGASRVVRDGGSAS
                     LQMGAITRTANNTLNAYGTLDLGAAGIATTSTTNTANILAIGSTAAITVNGTDWATGG
                     GSVNAFSAYVTTFANGQHTNITAALTNLTATTTTTNTLRFNTNASGTVSLNLGAASAL
                     AMVAGGILVTKNMTDNVVISGGTLRRSATTANLDLVFHHHGTGLLTVNSVIANNTGVL
                     ALTKTGPSTMILGGANTNTGRVNLQQGVLQVGDGTAGTANARLGNLTAVGTAGNPVSL
                     SEGATLLFNVANPSLVYTLGALNGGGLIHLAPTNTSTVMLVSTNTNWLGELRVDGGTL
                     RVSTDANALGNLRGLATIGGGGALEFFGVSQTYTKRLILEDGALIRAVPNGTTNTGST
                     LSGVLTLNNSTSAGANFQVAANSTLTLSNMIRSSNGFTKTGNGILTLSGNQFQDILPG
                     APVGITTPNVNAALLGQVVVSEGQLWVGNIRALGGTGPGNETRVLNGATLDLRGQALN
                     FVDSPDAFREVIHVSGTGVNGTGALRNSTGLGVFSHLVMDADALISGGGFLNASRLVL
                     GSYDTNTNTGSVLEGNFTRNRPTIDGNDRILTVRSSATTNDANGAGVTLRDPNFTSPL
                     QELVINEGILRIEKEFGPVSDFAGVTAANITNGITIAYSGPSLADKTNASLGVGPILS
                     SRLNLWNNWDVHHTVNIMMDGATAALNGGHNYIDTGAGTIPNSRTYLDGTITLTGPAS
                     RNVFHVDASSNNNTIVEQSNMTGAIQAKLIVGGQIVGAGGLTKTGFRELRLTNNNTFT
                     GDVNVLRFGTTAVRWQDNTVSVNGVDYQTFGDAEGWGEWGLTLAGANARLSGTGNVNL
                     QRRGMITLDNSNVLDATSGVAGGNHNDRINNAASINFENGWLRIMGGSSDNTEALATA
                     GGARLNVLSGSNFLDLMPTVDGTSMTLTIGEIARAPGSVLTFRNMDSTSTFGTLSGAD
                     TVRVQLLNIGSLAQVGAGTGMTSKKIIPGLLGGIIPHTLGEDIRELGFNNANASDYLT
                     QARNQQFLAGSHFMTFDGGILRPLTDSEYFTPAADGLIDSLNGSAGQNINLTETFTRV
                     RENVTINSLRFGPAADNNREGRNINDGTTLTDYTAAHNIQLYVDGTLKIDSGMISSAY
                     FTTGNSSSLATFIIGGTLNFGNREAVINNQNAMVRFTDGIVVTGNLEVRSAIAGNAGL
                     LKTGLAQVVLDGANTYRGLTSISNGTLFLRNGKSALGMGGPGNGVVIEGLGGLNSGNG
                     IQVGSPSAFEDILVKPVAGDIQVMRTENDATRWYSNVTIDNVDMAGMVVHLPRIAAAN
                     SATSMINGNIFGGNTTISHNIDATLSRIVQMDAGNNIMLFRGQIGDKADINGNPIPIS
                     DPISTLPSLAGTRTNQNEVLRVTLAGSVESNFTFERQYNAAGRIQLSNGNLLVTYDPA
                     AAGNDGGGFWTNTALSRIPNGDSTSSAFAVNGGTTSHGFTLVGGVVALTVPGQNFNMT
                     SWSTSGTTAKWVGGFNDSGNVIYGTPGNTGILTTTGVATRLYSMSGGTVTFDMSLAGN
                     VGTAPNNMGVIKVGRGNVVLRNTNQTAAATSNPQLSGGTLTLDYSGNNVALVGQQNAI
                     LAGGTLLAQSNLSTYSVANLAVTNAANSVLQLQLGATEVIAEGRGGQHMQISLGNMQG
                     AGTLTRALGATMNLVEWAKGTGTGGIALNFNSANTLLSLDASIPWATYSTTPRTAVDF
                     ATVSLTDPVAITANRTSGNVNLSNISPAGALQVGMVVTGTGYVVGTIVIAVPTPTTAV
                     LSIAPNATATAAAATARFNDVQQFNRPGGHFVNAVGSWTAGMDVSENGAGFTGALGGN
                     ETIKTLRFDTAANSQVTLNPGVALSFSGGGTLPGAILVSSNTGAANKTITGGTLSTSG
                     STELIFHQYGQGVLDVQSTITGTINTVINGPLTTDPGQVGTTGAVRFSANNTYDGRTI
                     ITGGVLEIGSVSALGVNPTDVSNDRLTLNGGTLRWTGTTASLQNRGIRLEGNGGVIDV
                     ANPDGNLVVGRALLGAQATLTSQEALRGDLIKTGVGALTFLGNQSLSGLMDIQQGTVI
                     TMVDTGDDGATAARTATVFGTNRSFADGTILRNGANVQVIMGNANNGQEWNIDEYFTF
                     EGGNTFTYGGLLDVNANLALSGVLDDQFNIGNRRPLNLNGWLKVDGTTTFTVTNNGVL
                     RLGNSAGYITGSGDIVKDGPGQLHFRTNTPDWKGGLVIREGSVYAANQADVLGSGHLT
                     GKTIILGDADRQFTAELLVQNPDSVSGSWMFDIYHDIEVVYNPAQTKRLGIDNISNGN
                     LVTFHGDVTMNDNLILLVRDVAIAAGGEQAYVNYNGSFKDGAVTSGNLLIQSNDSDAT
                     ANNLTSGRTYGYAILGGDNSAWTGDVTISSNLVNNQDNTSILRLAHSKALTEANEVVM
                     NFNSILQAGGQSVTIGSLNAQGGNGAFHGDAGTMSGNVNSSSEIIENAASTAGTLRIF
                     QSTPAGYESSWDAFFRDGTLNSEFLAPGANILRPSAALSIVKAGDGWATLSLHNDYSG
                     STVVEGGILQVGRNGIGSTGRTGALGTTVMSGGVIAGSGVVQGGMTLLAGATLSPGDL
                     AGRELGTLSVDGNAIFSTGSQTLMQIRLPSYNNPGAMNATDPLYSTWRNGVTTDSFSN
                     ALADLVTPVQHDMLHATGTINWGVGTQVTLINDGYTPKAGDIFRLFSGSGYAGAINVG
                     PSLRTGGETGTDLTLFELGGNFLWDVSLFNSQGILMVVEADSAPMIIPAPVITAGPSR
                     NPATGVFEPGDQVQLSVSATGAGTLLYQWYLNGAVVPGANQPVYQFQVNFNTKGVYSV
                     SVTNDGGTTVAPTTVLIEVDAVPFIAVHPSPVTVNPGQNASFTVLASGQAPFEYQWLL
                     NGVEIENATSQTLLLESVAEFDEGVYSVVVKNAAGEVTSNGAALTVNDPITQAIASRS
                     PFPRAYVGETITFSVSHDGTPRDTGSPFSYVWRKGGTPISGGTGETLVLPNANAMLEG
                     DYDVVVSNDVNSFTSTPVTLTLLAPAPVVEVQPTVTQTLLSGEPLMLAVQAGGRPPLT
                     YTWKLGTTTVASGSSNTLLIPQTAVSQGGIYVCEVSNSTTTKATSDPAEVVVVDSSTR
                     LTPVAIGGAAVLTAVVGAGPATALEYEWFKNGEVMLEEDFPAVTGIRERVLTLPDVTL
                     DDDAFYSCQVKGPAENSVLGCTHDVRVFTGAPELPLFAFPDATIHAPYLFEIPFNRAD
                     RTKTPSSVIATGLPPGMVMDTLTGTITGRPNKFKKGGYAVKIVVSNSFGSVEQTAILN
                     VNDLSGTLAGVWTGVMERNAVLNTGLGGRLDFTVTTLAAYSGKLTLGGVTYSFKGILD
                     VGPVTSKITATIPLKGKPVPPPLVLTLTLQGNEVLEGMVTNGTAECELLSGWRGIWGK
                     KAPLVPATEYAGYHTFGAALPAGSPLLGSTDVPQGSGYGSFTIAVDGKLKIAGRMPDG
                     EAISLSTFLGPDGGIGIFQVMYKAIKPGGSLLGSLELTTGGNADPADNTLMSDDLTWV
                     RPASTKATDRTYRNGFGIATSPVVEPVPLAAVGGFYKVPDAKANMVVLDMPMAPDTNT
                     MNAEILFSDAGDLENPALTRWNPNQQASVGKNSKVTTRKKQPLAVPPVNPAGTTVAAA
                     AKTGILTGGFTLEDRTPVGYVPVSTVKRAVKYQGIIINDGGKWVGVGYFLLPQLPQVG
                     PPFTTTKNSPILSGLMLFEEL"
     gene            29120..29482
                     /locus_tag="PLP58_01065"
     CDS             29120..29482
                     /locus_tag="PLP58_01065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ07140.1"
                     /translation="MPGFKFAVEFSEFSRMTRVSVSIAPETTTAEAFTQMDARLCSDQ
                     ERVQANINKVTGNFSRVRRQFMKITEGFLSGWADFMVLNLAGCIGPGVEGKVFCWQKS
                     FRVETDFFKLLIMNDFPE"
     gene            29779..30606
                     /locus_tag="PLP58_01070"
     CDS             29779..30606
                     /locus_tag="PLP58_01070"
                     /inference="COORDINATES: protein motif:HMM:NF015506.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="HRJ07141.1"
                     /translation="MIVMPFSVPPPLSVRLESGLGECRQRHGNWLQQEENLRPLAWLV
                     AFAGCFLLTGLAGILREDDWRMDFTPGSASAPAGLDAGIVETTLAELQSTVAAETLEP
                     DSTEVQFESPPVVEMPLELQDLPELIPALVTEDVCTVPAAPKIESVLTPVEPPKPRPV
                     VNRAPSPATRPPSQTSARPASPGGGMGGTGISGSGGSGRFPTPPYPSFARSRGIQGSV
                     MLTIIVNATGAVENAIVSGSTGYSELDSYAASWVRRNWRFPPGAARSFKLPVVFKLR"
     gene            30609..31295
                     /locus_tag="PLP58_01075"
     CDS             30609..31295
                     /locus_tag="PLP58_01075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009959478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HRJ07142.1"
                     /translation="MIFVLYPPVIPLPCPVLANAVIEFIVKGGPIMYPIVAVALFAVC
                     VLVERLLWWLRLATRRSPRQLEEVYAALEAGDPDQAIARSEKTTDPVVRMIHHGLKHP
                     HSSMQGALEVAAGHELQNAGRFLGAMDTIVTLGPLLGLLGTVTGIMGSFSSIGGDELA
                     VEKVTGGIGEALIATAAGLGIAIATLIPMNWFHSRLARLQFDLEAAANNVLILAARHG
                     FDQSAARTAK"
     gene            31312..31740
                     /locus_tag="PLP58_01080"
     CDS             31312..31740
                     /locus_tag="PLP58_01080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007416400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HRJ07143.1"
                     /translation="MKLHSPIPQKKARLEIIPLIDVMFFLLASFMMVSLTMTRQQTIK
                     VNLPVAAASEPDFKPDMINVAVSAAGDLYLDTTPVTLPDLEQRLAERRARSPDTPVYI
                     SGDADTRHADMVKALDIARRAGFSKVAFNVRATGPAPQQK"
     gene            31750..32298
                     /locus_tag="PLP58_01085"
     CDS             31750..32298
                     /locus_tag="PLP58_01085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ07144.1"
                     /translation="MKRFLLSLLVLITLGLCAVCALQWQREHRLRARISDLTRQLAEE
                     NKRRVEFEEKAMRYEQEIARVTTLRAQTEAALLEATETLQQTASDQMARGHSIAVLSS
                     ELLRARAESAALRELASQGTSAIQQRNETVAGQNAAIEKANARLRELAKERDDAIQQL
                     NARTREFNALVEKYNALAKGSR"
     gene            32368..32697
                     /locus_tag="PLP58_01090"
     CDS             32368..32697
                     /locus_tag="PLP58_01090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ07145.1"
                     /translation="MESTRVIQLPVRSQPAHEPELIKLVQSQGRENISGGDAESSRRF
                     ASEIQYVTWHARRIAPILGMSTLRLGIVEDREGQTAFHISSDGWHGVVSGGRRPLRQL
                     RESLARG"
     gene            32707..33411
                     /locus_tag="PLP58_01095"
     CDS             32707..33411
                     /locus_tag="PLP58_01095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ07146.1"
                     /translation="MNLDPLIAATATLQRLDGVAGILLFKGANVIHKQMPFSDARARE
                     LREALDEMLAGYREVRRKLRLACLEFDAGLLLILVQGGTALVFLLTAQADPDLVGSAG
                     LVLLEDHAEALEAAPAHSEAAPPAADEVEELIVTSPRRLEQITEKAAPVVNHWGAVRK
                     ALEGLLGKVMGRAQAANLVDRVLAEKGVDDPWRLPRPQIEKTALHVLDNIPNIAKRRQ
                     LATELRAALEEIHPDH"
     gene            33430..34869
                     /locus_tag="PLP58_01100"
     CDS             33430..34869
                     /locus_tag="PLP58_01100"
                     /inference="COORDINATES: protein motif:HMM:TIGR00836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008519 - ammonium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0072488 - ammonium transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HRJ07147.1"
                     /translation="MSLPRLLPAMLLLAGAAFSPLASQTPSPENPRIEAKLDTIITKL
                     DGVAAAVGADKTAPESPPPTDETGSAAQASVDLVWVILAGALVFLMQAGFGLLELGFS
                     RAKNSINIVMKNFLDFSIAVVVFMFLGFGLMFGSSAGGWIGTDGFWLSNFPEDSPLWG
                     FWFFQVVFAGTACTIVSGAMAERTKFVGYLVYAGFLSGVIYPLTGHWAWGSFGGAYGV
                     GGTGGWLESLGFVDFAGSTVVHGCGGACALAGILVVGARHGRFAKDGSPRMIAGHNIP
                     LAALGVFILWFGWFGFNAGSTLAASGGIGRIVVNTAVSPASGALAAMLSMWFVQGRPD
                     VGITLNGALGGLVGITACCANISPASALAVGLVAGILTTVSTIGLERLRLDDAVGAVP
                     VHLVNGWWGTLCVALFDQKGFDARQLGIQALGTFSITATSFILCLAVFKTVDLLVGLR
                     ATDEEQINGLDFSEHSANAYPDFQTTEQV"
     gene            complement(34885..35010)
                     /locus_tag="PLP58_01105"
     CDS             complement(34885..35010)
                     /locus_tag="PLP58_01105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ07148.1"
                     /translation="MFHELRIEVLDIPDEYEFMNPEIIVLIRERVESVLERKAGD"
     gene            complement(35164..35916)
                     /locus_tag="PLP58_01110"
     CDS             complement(35164..35916)
                     /locus_tag="PLP58_01110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ07149.1"
                     /translation="MPDQGVTWDQLLELERRKAIAEAKEQAKTEAEVSGKTNAWAKML
                     EEDNQEQSEKIRLLESQLASQAEETERQRSLAENYRFALEQRKKGHVENDQTTPSQYL
                     PPDTVAQAVENAERDHHEKLVFSWNNKGKPADCPYRFPDEVERAFNWLATTYWLARTG
                     KHPCADLDKSVRELIPGWSFSGGQKKHSVGKHESWYQCTWNRKDYWIGEHLGCGTSKR
                     PEETIRIAFAWDDEQKKIVIGFIGQHQRNSNT"
     gene            complement(36787..37272)
                     /locus_tag="PLP58_01115"
     CDS             complement(36787..37272)
                     /locus_tag="PLP58_01115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ07150.1"
                     /translation="MKLSPIQLLDYHVTSLTVEASVSFQCDKPMDLDPADLHVSASVR
                     LLNDGDDPRWAVTLSLRHEPVEGKNIPYRLVIELVGLVSTVMKKINDGVKHAVEVNGP
                     SMLFGAAREILREATARGPHGSIIIPSASFFTSPPVVEVSEEKAPQISAEKKKRQRPM
                     E"
     gene            complement(37269..37802)
                     /locus_tag="PLP58_01120"
     CDS             complement(37269..37802)
                     /locus_tag="PLP58_01120"
                     /inference="COORDINATES: protein motif:HMM:NF027067.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HRJ07151.1"
                     /translation="MKTTTKLHTKPFEIHSPATENRAARKVATIEVQVRVDEDGDEVL
                     TPESLNLIDKTQARYMGLLMGEDIRALRERLNLSQDELSDLLGCGKKSLSRWENGHGY
                     PSQLVNTLLRALDERLFTPAQLRGLQSPRADWGELISGTTPRSHRFQFTCTAASAWDD
                     CPATKRGELNFPSLFGS"
     gene            complement(37799..38188)
                     /locus_tag="PLP58_01125"
     CDS             complement(37799..38188)
                     /locus_tag="PLP58_01125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ07152.1"
                     /translation="MPQLSPTPIPDSWRQDVIRILKSGDSRCIEWTVRARSDWSVFGI
                     SHKAYEHLIQTLSTPALIGRQVLFMDGRTEVWEFLCPHPLGIVKPLYAKIGLKEGRLN
                     IKIFSTHIDLTGELQTAIQRLHKPRKR"
     gene            complement(38464..40011)
                     /gene="gpmI"
                     /locus_tag="PLP58_01130"
     CDS             complement(38464..40011)
                     /gene="gpmI"
                     /locus_tag="PLP58_01130"
                     /EC_number="5.4.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018968642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046537 -
                     2,3-bisphosphoglycerate-independent phosphoglycerate
                     mutase activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-independent
                     phosphoglycerate mutase"
                     /protein_id="HRJ07153.1"
                     /translation="MKKPVVLIIRDGWGINPGGKAQAQANGDATLLARTPFHDHLYAT
                     YPRGTVSASGEDVGLPEGQMGNSEVGHLNLGAGRIVYQDLTRINKSIRDGELAKMPVL
                     AEAFEKAKGKRLHFLGLISDGGVHSHQDHLAALCNAAKAAGVTDLMVHAITDGRDTSP
                     TGGAAYMAKLEKDLAPSGAKIATVIGRYYAMDRDKRWERNKLAWDAIVLGRGEVRTDL
                     PSAAVQAAYPKDPRGDEFMQPMIFSNANEQRIRDGDVILWFNFRADRARQLSDAFLKT
                     GFEGFDREVHPQVGYYTLTEYDATYEALGARVIFGTESLSNNLGQTIAAAGLTQLRAA
                     ETEKYPHVTFFFNSGIEEPNPGEERYLAISPKEVPTYDKKPQMSAPDLTFEVLRRLEN
                     HDLVIMNYANPDMVGHTGVVEAGVHACETIDLGVRMIVEKVLELGGKLFITADHGNCE
                     QMRNPDGSPHTAHTTNLVHGIYVAADAANVTVRDGILADIAPTLLDMLGVKQPEEMTG
                     RSLLVRN"
     gene            40113..41084
                     /locus_tag="PLP58_01135"
     CDS             40113..41084
                     /locus_tag="PLP58_01135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ07154.1"
                     /translation="MSDPIFIHEYLLRSGAALNSASQRRVFPGALIRVGAGYGCVHPW
                     PEFGDAPLDEQLAALARGITTPLTALALNCAAADGAAREAGVSLFEGLEIPRSHYSWS
                     FAQATQPQFDRVLEEGWPAIKAKGWANYGETTRFLDNCARMSGDSGPLFRVDFNACLD
                     ADTFQKFIEFMPLRVYRRLDLVEDPFPYDAAAWEVCRVRWGVRLALDKRWQEGVGGFD
                     AVVVKPARRDWRAVAVRHPGHPLMLTSAMDHPLGQMFAAWEAAQARAALGERVGLCGL
                     CTQHLFEPDAFLERVLSPGGELRPDTDGGGLGFGSVLESLPWRRLEA"
     gene            41189..42208
                     /locus_tag="PLP58_01140"
     CDS             41189..42208
                     /locus_tag="PLP58_01140"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HRJ07155.1"
                     /translation="MCFFQTSGSEGLPKWVALRKEAILISGQAVNAHFEASSNDRWLL
                     ALPLHHVGGFAILARAHLAGCGVARLEGKWSPRAFAEACAKTTLTSLVPAQVHDLVRE
                     NLPCPAAMRAVVVGGGGMTPELAAAARALGWPVCQSYGMTEAASQIATQAWTSDGPAG
                     GVDTLAVLPHWQTSTEADGRLRLRGPALAAGYVNRAAGGDWQWQPLDPQNGLLTRDHA
                     RLWDADGRRWLSFIGRETGYVKILGELIHLAPLQARLESLAIELSWPMMPILVPRPDA
                     RAETELVLVTEHGCPDAGPLLERFHQTCPPWLRVRETRVLNAMPRSSLGKVCEDELRR
                     LVAEE"
     gene            complement(42233..48367)
                     /locus_tag="PLP58_01145"
     CDS             complement(42233..48367)
                     /locus_tag="PLP58_01145"
                     /inference="COORDINATES: protein motif:HMM:NF019298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004866 - endopeptidase inhibitor activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MG2 domain-containing protein"
                     /protein_id="HRJ07156.1"
                     /translation="MKNLLRLCLAAAMSATLHAQQSGDPPAKIRDLAAQNNHREAADL
                     GKQVLASTQDAGLAAEVLDLTFESLSSLGAHVEQEKVIEDAAARFPQSWRVLLNAGIR
                     VLRLPSYGEVLDGEYRRGGYRGGGRHVQSQIRDRTRALQLLEAAWKALPADATSPVRL
                     DVLHGLESALRHGRNYFHQAWSLQVLTDLSELPDYDDQSGLDSSIGGYPVDAEGHPVF
                     FKAAQSWESAASDGERLLWIMEERERMEPASVWQHRQTRAVMARQWFSVNTLAYYGIR
                     FDSSESDESETRGGIASLHTLKDGETTARLTTGAKRFELPAEWAFLHLLRAVAESADA
                     PDYLRVGAWSQIAEEMNDRRQYPRASEALRQAIALEKDTNQKAQLQSRLDQITGNLGR
                     FDPLPPQPAGTEAKLSLVFRNARKIELSARRVDVGRLLDDTEAYLRGEPQDRDWQKTN
                     LGMIGRRLLEEGGDKYLAPVSHTWTQGLEPRANHWDRRVDVPTPLKEAGAWLVEGAFE
                     GGGKARALLWLESLNIVHVRQPDGGLYYIADAVTGEPVRDARVKFMGYRSEWARPGLL
                     NKPRMTYNFKDFQASPDEHGQIQVKRAQMENYQWLIRADTKAGGLAFLGFAHFYFGGS
                     YERFNEQAKLYAITDRPVYRPGQEVKWKAWARMVGYDSKLDTNAFAGSQIKVTILDPR
                     GEKLVEKTYKADDSGAVSDALTLGEEAALGVYNVQFEHTRALRGVVHLGSHSFRVEEY
                     KKPEFEVKVDAPVTPVALGDSFEVKVKADYYFGGPVKQGTVKYKVQRSAHTSRWFPAG
                     RWDWLFGSGYGWRATYYDWYPGARSWCFCIPRWPWIRWQDDPPELVAEGEAALNADGT
                     FTVKIDTALAKELHGNEDHRYEIEAEVTDQSRRAIFGKSSVLAARRPFEVYVSTDRGY
                     YTAGQDAEFSVHARTLDGREVAAEGTLVLYRVTYDQDGKPAEEAVHTADIKLDESSFT
                     RNVKTTLARGGQYRVSVKLKDKAGHEIEGVTFLTVRGAGFDEGKDFRFDDLELLVERE
                     EYAPGDEVEVTVNTNRPGSTVALFLRVQNGLYPDPVWLHLEGKSTTHRFKITEADQPN
                     FFLDAFTVNGARVHQVTRQILVPPKKRIATVELEPDAETYLPGQGSKVKVRVKDADGK
                     PFTGRVVLTAYDKALEYISGGSNQEDIRPFFWGWKRSHYPQVRDTLHALETGLLKDGE
                     KWMEALGIFGGEVADDFGNMDRQTAKNTYSGAVGGMAMRRGMPMPMAAPAPMAVMSTA
                     AEAAPMDKAAAMPAPGGGGAPEEGPQPMIRTNLADSAVWVADFTTDAEGMGELAFNLP
                     ENLTTWKLRSWVMGPQTQVGEASVEVITRKNFMVRLQAPRFFVEKDEVVISANVHNEL
                     DTAQDVRGVLELEGGVLEFAVAPQTASVQVPAHGEHRFDWRVKVTGEGEARLRVKALA
                     QKDSDAMEMTFPAYIHGMLKTDSWSLALRPDQASGSLTLNVPAERRPRQSRLEVVYSP
                     TLAMTLVDALPYLASYPYGCTEQTLNRFVPTVITLKAIRDLGVDLKKVRERLKDAAAQ
                     APMPPGEQKRLREAVFSEAEVEKMARTGVSRLLAMRNADGGWGWFPGGRESSAHITAL
                     VLHGMKAAERAGFDLNEGLIRNGVEWLTRHEAEQLRRLGLPQKDREHKSAPDNLDALI
                     HSVLVEYSGGDPAMRARLYDQRAGLSRYNLALLGLACDATGERERRDMCLRNLRQFLK
                     QDDENQTAWLDLPEGGWWWFWYEDRIETQAAFLRLLVKAEPKGETAARLAKYIVNNRR
                     NGTWWTSTKDTAAAIEALAEFAKASGESAPAQTVEILLDGVPQKRVEITPDTLFTFDN
                     RFVLEGEALTTGAHKLQLRKLGASPLYVSAYLTVFTQEDMIPAAGLEVKAERRFYRLI
                     EEKPDQQVAGARGQVVTQRGLKYRREALASDAEIRSGDLIEVELSIESKNDYEYVLIE
                     DMKPAGFEPIEVRSGWSYEGLAAYREFRDERVAFFAERLPRGRHNLSYRVKAEIPGRF
                     SALPARIEAMYAPELKGNSQEWKARIVE"
     gene            complement(48509..49606)
                     /locus_tag="PLP58_01150"
     CDS             complement(48509..49606)
                     /locus_tag="PLP58_01150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007416055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HRJ07157.1"
                     /translation="MHDPRVDQLARQLVQYSTQVKKGDLVWLDCYDVPNCVPLALIRA
                     VVAAKARPVVRLHDTRVTREMMIHADDSQYEIIGGSDLDLMKKMDCYIAVRGSHNITE
                     NSDVPADKMKMVMARLRPLQNERVNNTRWCVLRWPTAAMAQQAGMSTEAFEDFYFKVC
                     LLDYAALIPAMNALKKLMDKTKEVHITGPGTDLKFSLKGLKAIACGGRHNIPDGECFS
                     APVRDSVEGVISYNAPTIYQGIAFDSVRLEFSKGKIVNAAANNTEAINKIFDSDEGAR
                     YIGEFAIGFNREIREPMRDILFDEKIAGSFHFTPGQAYEGIADNGNRSQVHWDLVCIQ
                     RPEYGGGEIRFDGRVIRKDGKFTLPELKPLN"
     gene            49740..>50732
                     /locus_tag="PLP58_01155"
     CDS             49740..>50732
                     /locus_tag="PLP58_01155"
                     /inference="COORDINATES: protein motif:HMM:NF037008.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HRJ07158.1"
                     /translation="MSLPSSEDSGTRERILLKAAELFAQRGFESVSLRDLTQAAEVNL
                     AAVNYHFGSKEALIEEVVTRVMRPMNDERLRLLDAAEAAAHPAPAPAEQVADAFLRPI
                     MLMVRESPLSEMLFFKLMGRCLADRTNLIPAAAMPHFEQMALRFAKALRRSNPALTHA
                     EACWRLNFTAGALISTLAHADIAMRFAGELENPPDTETVLRRLVAYCTGGMESAPAGR
                     KRARGPRPVKAAVLGLGLAFVLSACGSLSPKQRMDELDLEVPGTWAASKEARSGVDEV
                     WIKRFKDPRLEALVDEALQNNPDLRAAAARVERARGQTRLAGVAGNPTAELKFDP"
BASE COUNT         9532 a        15762 c        15250 g        10188 t
ORIGIN      
        1 cccctgcccc ggccaagaag gaggaaccca aaaaagacga ggcaaagaaa accgaaccaa
       61 agaaaggagc ggccaaaaag gaggaaccca aacccgccgc cactccccct gcgtcaaagc
      121 ccgccaatac tcccccgact ccgaaacccg ctgacgcgcc gccggcaccg aaacccgccg
      181 ccaaacccac cgacgccccg ccggcaccca aggccaattg aactcaatag acttggcgga
      241 cattttccgc actgtccagc aggctcagac tgccatcggg aagctccagc ttgagccttc
      301 cctcctcatc gagatctagc gctcgcccga ccacttcacg cccgtgctgc caggcgcgaa
      361 tttgcctgcc aagcagcaaa ctgcgtgaac gcgcctccct cagcgcaacg ctgaatccag
      421 agtcaagccg ccccaggtga ctgtgcaaag cgcttagcag ggccaaagcc agctcatgcc
      481 ggtccagttc catcctgact tcatcgccaa gctccaaggc aagcgaggtg gcctcggagg
      541 cgattcccgg cgggaaaacg cgcgtgttca cattcagccc gatgccaagc acgagcatga
      601 gaccttcagg accgttcagt gtctcggcaa gcagtcctga aaccttgcgc cttccaagaa
      661 aaatgtcgtt cggccacttg atgacaggac gtagtggcag cacgcgctcg atggcctggc
      721 aaacagccag cgccgccagt gttgtcacgc gcggccaaag caccggaggc gcagcgggcc
      781 gcagaagcag ggagagcatg aggtcgcgcc ctggcggcgt gatccagcgg ttttcacgcc
      841 gcccgcgccc ggcagtctgg ctttccgcaa acagcacagc gcccggcggc tcaccgcgca
      901 gcgcgactgc gcggaggtcg tcgctggtgg acccggtctc ggcggtcact gagacacgcc
      961 agggcaggct atcccgctca caggcatctt gaagaacggc tgcatccaag acgggcatgg
     1021 catttcaagc atcaggatcc cccgcatgga ccttgtcgtg gtcgccgcag gtttcgaggt
     1081 gggtgatgat ctgcattttc accggcatgg cggcgatgac ggcacgctcg atctcggtgg
     1141 cgcggcggtg cgcgtcgcgg agcagaatgc cgtccggaaa aagcaaatgc acgtccacat
     1201 gatggaggtg gccggagttg cggtggcgca gcgcgtgaaa actgacgccg cgcgcggatg
     1261 tctcatgcgc gagcgcctgc tccagttggc gggagacggc gggatccgcc tcgtccatga
     1321 gaccggtgac actttcccgc acaaggcccc agccggagaa aaggatgttg gcggcgatca
     1381 agagaccgaa aagaggatcc caccacagac tgcctgtcag cacggtcatg gcaagacccg
     1441 cgagcgcgcc aagactggtc catgcgtccg tcagcacatg acgtccgttg gcgcggagga
     1501 tgaacacgtc aagccggcgg ccagcgcgca tgaggaaaag cccaagcgcc agattcacca
     1561 ggatggcaac gccggtgagc accatgccct ggtccaggtt caccaccccc agcccagaca
     1621 cccagcggcg taccgactcg aaggcgatga acacagccgc cgccagaatg agcgccccct
     1681 ccactccggc tgaaatgaag gccactttgc tgtggccata tggatgattg gcgtcaggcg
     1741 gcctctggga tagagccagg ctgaaactgg caaaaaagac agccgccaga tgcaccacgg
     1801 actccgccgc atcccccagt atggccgccg agccggtcaa ggcccaggcg ccgagcttca
     1861 gcgccagcat gaggaagctg gccgacaatg accagcgcat ggccctgcgc gcggcggcct
     1921 gggcttgggc gtgcttcatc atgccgcaag catggcccaa accgcgaaaa ccgccaacca
     1981 tgtccgcaga ccgcggtcaa gcctgcttct gaccatcctc atgatgatcg tcgtcatgat
     2041 ggtcctcctc gtcatggcgg tcctcgcgcg gatcatgccc ttcctgatgc ttgatgtccg
     2101 catgctcggt gacgcctttg aggtgggtct tgaatttgcg cacgcgctgg cgctcgggca
     2161 gcgggctgat ctgcatgttg atgttgcgcc cggcctgctt gggctcctgg tctgcgtgcc
     2221 ccatggtctg aaggtcggtc ttgatcttgg cggcaagctc gtggcccagc tcctggtgcg
     2281 ccatctgccg gcccctgaac tggagttgaa tgcgcacttt gtgcccctcg gccaggaagt
     2341 cctccgcatg gatgatcttg atctggtagt catgagggtc aatgccgatt cggaacttga
     2401 gttccttcac cttgccgccc ttgtggtgct tgtgggactc cttcttgtgc ttctcctgga
     2461 tgtatttcca cttgccgtag tcaatgattt tgcagactgg aacttcgaga ttggcagcca
     2521 cctcgacgag gtcgagaccg cgctcgcgcg ccatgcgcag cgcctgcccc gtgggcagca
     2581 caccgtattg ctggttcgtc gccccgtcca ccacccgcac cttcggcgca cggatgcgct
     2641 cgttgacgcg cgtctggtcc gcgtagcggt tgttgttgcg gttgccgcgc ggcggcccga
     2701 aacccgcccc catattgccg cggaaagcgg gcgcgccgcc agcggaagca gtccgctggg
     2761 acggacgggg aggacttcca gccgatcccg ccggcctgac ttgcccctgg ccgggttgat
     2821 gtgactgccc gccttgagcc ggtttgaact ggctgctatt gatgttctgg aatggattgc
     2881 ttatggggaa cctccgggtg gttttgattc aaagacgaat ctctctttga aaaatctggc
     2941 aggctgcgcg gtgaatggct gacatatcaa gccgagtgaa cctggtggtg gatggacagc
     3001 cattcctcaa cccgatcatg cgggagatgg gaatgtgatg ctgcgtatgt catgcgtctg
     3061 gaaaatcatg ggtggaagcg gatgtcttca tcgctgcctc cgggcggaat agtccgccgc
     3121 tgaccacaac gaacttgata gtcggattct tagcgcgcgg caagacaaat tccggcaaag
     3181 tcctcgtcac ccagggattc tgcggaaatg gggacgaatg aatggaagcc tccggagcca
     3241 gccacgcgcg ggtggtttcg atcatccgca ggcaggcgat attattcacc acgcggccgc
     3301 agtcagtgtc gaaaaacttg atatcttcgc gggcctcaat ggttgggtat gcatggtccg
     3361 cccatttttt tggaggccct ggccatcccg caatcgactt ctcccggtcc gtgaagttct
     3421 tttgaaatgc cccttgccaa tttggattac acatgtaatc catgtgggtc acaagtgtaa
     3481 tcctcgcgca tgaatttctt gtcttcacct cactgcgcca tactcatctc cacagtgctc
     3541 ctgtttggag tctctggtca cgcagccgag aagtacgaca accccacgcc gtggtcctac
     3601 cagcgtctcc agcgtccggc tattccggcc accacagaca ccgcgtggcc gaaggacgat
     3661 ctcgaccgct tcatcctggc ccggctcgaa aaggaaaacc tccgtcccat cggcgacgca
     3721 cctcgcgcca tcctcatccg tcgtgcatcc ttcgatctgc gcggcctgcc gccttctcaa
     3781 agtgaggtcg atgcctttct gcgtgatcca tcatctgatg acgccgcctt cgcgaaggtc
     3841 gtggacgcct tcctccaatc cgaacgcttc ggcgagcgct gggcgcggca ttggctggat
     3901 gtcgtgcgtt atgcggacag tgtcggtcgc gtgtggaatg cgccgtttct gtatgccgcg
     3961 cggtatcgcg actgggtgat tgattccttc aacgacgaca agccctacaa ccgcttcgtc
     4021 gccgagcaga tcgctggcga tttgctcccg gcgaagaccg tgctgcagcg ccgcgatcaa
     4081 atcgtcggca ccggcatgct cgcgctcggc agcatgaatc tccaggaggg cgactacgag
     4141 cagcacctcc tcgatcaagt cgatgaccaa attgacgtcg tctcacgcgc ctttatgggc
     4201 atcaccgtcg cctgcgcgcg ctgccacgat cacaaaacgg agccggtcac cacgcgcgac
     4261 tactacgcgc tcgcgggcat tttttacagc agccgcacac tcagcggcac gccgaaccgc
     4321 aacgacggcg tcgccggtgg ttacgtcagc cccgaggcac tcgtcgatct gcccaccgag
     4381 ctcgatgaag ccgtcggacc accgcagaag ctgcctgcag gtctgcattc gatggatgac
     4441 atccgcgagc tcggcaatcc gaagcaaacc atccgctacg accacgatcc gcacttctgc
     4501 atgggcgtca tcgacgccga aatcaaagac tgcgagatcg ccatcggcgg cgacccgcat
     4561 gatcgcgaca ccgcgccaaa ccgcggcgaa ttaaagctcc ccgccttgcc accgatgccg
     4621 aaagtgcccg cgcaaagctc cggccgcctc gaattcgccc aatggctcac gcagcccacg
     4681 catccgctca cctcacgcgt catggtgaac cgcctctggc agcatctttt cggcgaaggc
     4741 ctcgtgcgca cgcccgatga cttcggcatc accggcgccg atcccacgca tcccgagctc
     4801 ctcgatcatc tcgccattcg tttcgtcacg aacggctgga gcgtgaagaa aatgatccgc
     4861 gagatgatgc tcagtcacac ctaccgactc gccaccaccg gcaatcccga gcatcccgat
     4921 gcgggcaaca aactgcgctg gcgtcaaaac ccgaagcgcc tcgaactcga accactgcgc
     4981 gacacgcttc tgcaactcgc cggccaactc accttcgacc gccccgaagg catccaagtc
     5041 gccggcaccg ggggcaaagg tcgtcatggc atcacgcgcg gcctgctcgg catcgaggag
     5101 ccctatcgca ccatctacct ccccgttgtg cgcgacaaca tccccgaact cttcagcacc
     5161 ttcgacttcc ccggccccac ccaaatcaaa ggccagcgcg atgtcaccac cgtcgccccg
     5221 caagccctgt tcttcatgaa caaccctttc gtcgaacaac tctccgccca aatcgccgag
     5281 aaggccggca aagacaccca agccatctac aagctcattt tgggccgcga tcccacctcc
     5341 gaagaaatca ccggcgcccg cgatttggac gaccaaaccc tcgtgcaggc tcttttgggc
     5401 acggctgagt ttcgatatgt gttttgaagc catgaacgcg ctcgcatttc atcgtggttt
     5461 gaagcccaac gggcttctgt catgcagccc agggttgcgc gaagcgctac cctgggtcca
     5521 acgcgtcacg ttcacctcag gtgcatcaac cccaacgggc ttgcgtccgc cgccgaacgt
     5581 tccatcggcc tctgtttgcg atagagcctt cgatggacgg aaccccgttg gggttcgttg
     5641 tgatatgtcg tggggtctat cgctgaccca gggttgttcc aaccctgggc tgcatgacga
     5701 aagccctttg ggctttgaag gatcacgaac ccagtcttcg gccccaacct caaccttgcc
     5761 agtttcatga ccactcgtcg ttccatcctc caatccacca gcgctggctt cggctggctg
     5821 gcgtttaatg cgctgcatca gcagtgggcg aatgcggcgg ctccgccgaa gacgatcaat
     5881 ccactggctc cgaaggctcc gcatttcgcg gcgaaggcga aacgcgtgat cttcatgttc
     5941 atgcagggcg gaccgagcca tctggacacg tttgattaca aaccggagct ggtgaaggcc
     6001 cacgagcaga aggtggcgaa atacatgggc accgcgttcg acttcaaacc gcgtggcaag
     6061 agcggcctca tgatttccga cgcgtttccg gagctttcga agcacgccga cgacctctgc
     6121 ctgctcaacg gcatgcagac acgcacgaac gcgcatcaga tggccaccgt ggcgctgcat
     6181 atcggcagcg aaacgtttgt gcggccttcg atgggagcgt ggatcgttta cggcctcggc
     6241 accgaggcgg agtacctgcc aggctttgtc accatcaatc ccgtggccga caccggcggc
     6301 gcgatgaact acggctcagc ctttttgccc gcgagctttc aaggcacgcg actcagcacc
     6361 accggaggcg gcgttcccga tctctcgaat ggccgcctaa gcgacgcgca gcagcgcaag
     6421 cagatcgaat tcatccaaaa agccaatcgc aggctcctcg agggcgatcc cggcaatccc
     6481 gagctcgaag gcatcatcca gagctacgaa ctcgcctaca aaatgcagac cagcgtgccc
     6541 gacgtgctcg atctcgacaa ggagcccgcg cacatccgcg agctctacgg cctcgacaac
     6601 ggcgacaccg agcgctttgg cacgcagtgc ctcatggcgc gacggctcgc cgaaaaaggc
     6661 gtgcgcttca tccagctcac cagcaccggc tgggaccatc atcagcagct ccgcgaaggc
     6721 atcacccgcc aggccacatc catcgacaaa cccatcgccg gcctcatcgc cgacctgaag
     6781 cagcgcgaca tgctcaagga cacgctcatc ctctggggcg gtgaattcgg ccgcggatcg
     6841 agctacgaca acgaacccta caacggccgc ggacacaacg gccgcggcta caccatgtgg
     6901 atggccggcg gcggcgtgaa aggcggcttc gcctacggca gcaccaacgc catgggcgac
     6961 accgccgaaa ccggcatcat caccacgcac gacctgcacg ccaccgtcct gcacctcatg
     7021 ggcatcaacc acgaacgcct cacctaccgc tactccggcc gcaacttccg cctcaccgac
     7081 gtgcacggcg aggtggcgaa ggatattctg gcgtgataag gaggcggtct cacgacggat
     7141 gcgtcaggca aaatgaacga attacccgaa agaactttgg attgccaaat tcatgccgaa
     7201 tggataaatc agtgacacgt cgaactccga cgaagaaaaa acatcgtcat gaaatccata
     7261 ctcgccattc tcgcactcgc agcgttctca tccttgtccg cagcggaacc tctgaacacc
     7321 atctgtccgg tttctggaaa accggccaat ccctcgatca ccagcaacta cagcaagacc
     7381 gtggcagtgt gctgcgaccg ctgtgtgtcc cagttcaatg ccacacccaa ggcctatctg
     7441 cggaacattg tcactgccaa cggcgtgcag tgccccctca gcaaaaagaa ggcggacgct
     7501 tcgaagaaag tgacctacag ccgccaggtg gcctttgcgg acgcggccag caaagcggcc
     7561 tttgatgccg cgcctgacaa gcacatcatc gaagtgaggc agtgagtgca ttcgtcaaaa
     7621 acacggcggc tcccggcaag gaagccgccg ttttctttct tcgacagaga atcaggccgc
     7681 ttcgtcgcgg ggttcgatga ggccgtattc gccgtccttg cggcggaaca tgatggccag
     7741 gcggtgtgtc ttttgattgt ggaagacaac gaagggacgg tcgctgatct cgagatccat
     7801 gatggcctca tccggataaa gagggcgcac cttgtaatgc tcgttgtgga tgtaggcggc
     7861 ctcgacgtgc tcctcctcgg aatggagatc gtccgcatgg aagacgtgct gttcgagatg
     7921 acggatgctg ccctccttgc gcgggcggtg gctcttgagc aggcgggttt tgaacttgcg
     7981 catgcgccgg gcgatcttcg cgatggattc atcaatcgag gcgtagatgt cctccgtggt
     8041 gcttttcacc tcgatgacaa tgtggttggc gcagaaaagg atgatctcgg caatctggcg
     8101 gtggtgttcg atgtcaagga tcacctttgc ctcaatgatg cgcgggtagt cgagatgaag
     8161 accctcgatt tttttgtggg cgtagtcacg aatggcttcg gtgatctcga catggcgtcc
     8221 ggttacgatg accggcaggt tcacgttgtg tttttgcatg gtatgtgtcc tcctgatgta
     8281 ttgggggttg gtggttgaaa tctgtcacat ggtgaagccc tcgccaagat aaagcctgcg
     8341 cgcctcaggg tcgttgacaa gaaaatcctt ggagccatgc cgtctgacct gcccctcgta
     8401 gatgaggtag gcgcgatcca caatgttaag ggtctctcga acattatggt cggtgatgag
     8461 gatggcgaga ccagcctggc gcagcatgcg gatgatgtcc tggatttcgc tcacggcaat
     8521 ggggtccaca ccgctgaaag gctcgtcgag catgagcagc ttcggctcag tgacaagact
     8581 gcgcgcgatg gtcaaacgcc gtttttctcc acctgaaaga gtgagtgcca ggttgtccgc
     8641 gacatggtca atgccgaatt tctccatgag ttgctggcac tggtcctccc gctcctggcg
     8701 gctgaaattc tgcgtctcca tgaccgcgat gatgttttcg cgaacagtga gccggcggaa
     8761 aatggactcc tcctggggaa gatagcccat gccaagacgc gcccgtttat acatgggcag
     8821 ttgtgtggcg tcgctgccgt cgaagacgac tctgcctccg ttgggcctga caagaccgac
     8881 gatcatgtaa aatgtggtgg ttttgccagc cccattcggc cccagcagac ccacgatctc
     8941 gcccgcattg acctcgatgt tgaccccgtt cacgacggca cgtccgtcat aaaccttctt
     9001 cagatcctcc gtgtgcagca gcatcccagt cccgctttcc tgttctggaa tttcgcggat
     9061 gcccctgctt gtgttggatt tgccagccgt catgcctcca gcctcctcaa cgcccggagt
     9121 tgccgccctc ccgcgtgtcc gtgctcttgt cctcgtcatt caaaatgatg gtgcggggcc
     9181 gtcctgtggt gcgcagtttg ccattcgggt caaagatcat caaagtgtcc ggctgggtgg
     9241 caaggtgcag aatgttgcca cgctggacct gggggtagtt gctcatgatg atctctccag
     9301 tgccggcttc ataaacagcc ttccggcact tgccgatctg cgtctcgccc tcctcagtga
     9361 gtttttcgat ggtcacggtg gagccggtgg cagtggcttt tttgacaccc gcattcccgg
     9421 tctttttctc ctgggtggcg ccgtcatccg gccctggctt ttttttctga ctgccggctt
     9481 tttcttcctg cgtgtcggat ttgtctttct ccttgtcctc ctcgttctcc atttcgacaa
     9541 ccaattcgtc gcattcaata tagaactggg gatgtcgggc gcggacacgg cctgtgtaaa
     9601 caaacaccgc ctgtttcaag tcgaacaccg agctgtccgc ttctatgacc acggtgggca
     9661 cacggggttt ggaggccgca tgatccagag gaggtaaagt ttggggctgg gggccggagg
     9721 gctgttgttt cgccgccccc cccttttcac ccaccgattc gcctgatgag ccggaatcca
     9781 tcctttttcg cgcctcgggc agaagcgagt tggcggcgtc cacggcctgt tttttcagtt
     9841 cggacgattc cagggctgat tttgcggctt ccgcgacccc tgcgggtatt tgttccgaca
     9901 tttgcctggc ggcatcgccg gccttctcaa gcgcgccatc ctccctggcc tgctgaagtt
     9961 tttgcgccgc gtcccgcaac tcctttttca ccgccggcgc gtcttgcccg tgcgcgtcca
    10021 ccgccatgag catggcggtc agcagaaggt ggtgaaggtt tttcatctca gtcagcgccg
    10081 ggttgtgatt gcttcgattg gcccgtttgc ggtcccgtgt caaagatgaa tgttttgacg
    10141 cggcccttca tcgccccgat tgaggtggtc gcgtcaaaca ccatggagtc gccctccgcc
    10201 tcgaaatccg aacgaatgac acgactgcgc tccccgctgc gcacgacatt ggcggtcatg
    10261 tgaaaaatgg ctccgggcat ttccacgcgc gtgtcctgtg gcggcgtgtc cccataaatt
    10321 tcgatgaccg tcctgcccgc ctccagccgg tcgtcatcca ggcggaccag ggtcgcggcc
    10381 gtgagccggg atccgagttt tccatcattg aaagagggga tgaccacgtt ccggttcaca
    10441 aatccctgcg ggatccattt gccgaaggcg ctcatgctgc tctccgcctc ctgactgccc
    10501 gcgcagaaga cggcgagaaa gtatccggcg ccgatgcaag cacccgcaag cctcatggag
    10561 cacctccctg cttcacaatt tcatgaatca gcgtcaaagt gcgcagaacc ggggccagtt
    10621 cgtcgagagg aacctggttg ggaccgtccg agagggcttt ctccggatcc gggtgcgttt
    10681 ccatgaaaac accatcgcat ccggccgcga ccgccgctct tgcaagcacg ggcgccagct
    10741 tgccatcccc ccccgtggcg ttgccaaggc cgccgggtct ctgcacagaa tgagtggcat
    10801 caaaaatgac tcggaagcca agctcgcgaa tccagtaggg tgatctcatg tcggccacga
    10861 ggttttgata accgaacgtg gtgccgcgtt cggtgaacaa aaaattttca caccccgcgg
    10921 cagcgagctt gtcagcgatg tttctgacat cccagggagc catgaactgg ccctttttga
    10981 cattcaccgc gcgccccgtc tcgcccgccg ccatgatcaa atccgtctgc ctcgacagaa
    11041 aggctgggat ttgcagcaaa tccacatgct ccgccgcaat ggccgcctct ccaggagaat
    11101 gcacatccgt cgtcacaggc acgctcagtt tcctgcctat tcgggcaagc aactggcagc
    11161 ctgcgcgcgc acccatgccg cggaaagacg ctcccgaact gcggttggct ttgtcatacg
    11221 aggccttgaa cacccaacgc agacgcaggc tcgaggccag tgccgacaac cgctcggcta
    11281 ccggccagat aaactcctca ctctcaatga cgcacggtcc cagaatgaac aacggctccg
    11341 tgccatccac cagcacggaa cccaccttga ccacaggaag cgacagcatt tgctatgatt
    11401 gattgaccgg cggaccaccc gctcggcgaa gcagataacc gagcctgccc ggtctgacaa
    11461 ctctcaagag cggagaactt gcgcggcttt ccaccgcgca agcctgccgg gatcagaggc
    11521 cggtcacaat tcctcaaaga gcatcaatcc ggaaaggatc ggcgaattct tcgtggtggt
    11581 gaagggcggg cccacctgtg ggagctgtgg aagcaggaag tagcccactc cgacccactt
    11641 gcctccgtcg ttgatgatga tgccctggta tttgacggcg cgcttcacgg tgctcacggg
    11701 cacatatccc acaggtgtgc gatcctccag cgtgaagccc cccgtcaaaa tgccggtctt
    11761 ggcagcagcg gcgacagtag tgccagcagg attcacaggc ggcactgcca ggggttgctt
    11821 cttgcgggtg gtgactttgc tgtttttgcc gacactggcc tgctggttcg ggttccaccg
    11881 ggtaagggcc gggttttcca ggtctccggc atcagagaac aagatttccg cgttcattgt
    11941 attcgtgtct ggtgccatcg gcatgtccag cacgaccatg ttcgccttgg catcaggaac
    12001 cttgtagaag cctccgaccg cggccagcgg cactggctcg acgaccggag acgtcgcgat
    12061 gccgaatcca tttcgatagg tgcgatccgt cgccttggtg ctggccggtc ggacccaggt
    12121 caggtcatcg ctcatgaggg tgttgtccgc agggtccgca tttccaccag tggtcagttc
    12181 cagggatcca agcagggaac cgccaggttt gatggcttta tacatgacct ggaagatgcc
    12241 gatgccgcca tccggtccga ggaatgtgga gagactgatg gcctcgccat ccggcattct
    12301 gcccgcgatc ttgagcttgc catccacggc aatcgtgaag gagccataac ccgacccctg
    12361 cggaacatcc gtgctgccca gcaggggact gccggcgggc agggccgctc caaaggtgtg
    12421 gtagccggca tattccgtgg cgggcaccaa gggggctttc tttccccaaa tgccacgcca
    12481 tccagacaga agctcgcact cggcggttcc attggtgacc atgccctcaa ggacctcgtt
    12541 gccttgaagc gtgagggtca aaaccaaagg cggcggcacc ggtttgccct tgagaggaat
    12601 ggtcgcggtg attttggagg tcaccgggcc gacatccagt atgcctttga aggaataagt
    12661 aacgccgccg agggtcagct tgccgctgta ggcagccagg gttgtgacgg tgaagtccag
    12721 gcgacctccg agtcccgtat taagcaccgc attccgctcc atgacaccag tccagactcc
    12781 ggccagtgtt ccgctcaggt cgttcacatt aaggatggca gtctgctcaa ccgaaccaaa
    12841 ggagtttgag acgacgattt tgaccgcata gccgcctttt ttaaacttgt tcggacggcc
    12901 cgtgatggtc ccggtgagag tgtccatgac catgccgggc ggaagtccgg tggcaatcac
    12961 agagctgggc gttttcgtgc ggtcggcacg attgaaggga atttcaaaaa gatacggtgc
    13021 gtggatggtc gcatccggga aagcaaagag cggcaactcg ggcgcgccgg tgaagacgcg
    13081 cacatcatgc gtgcatccga ggactgaatt ttcagccggc cccttaacct ggcaggaata
    13141 gaaggcgtcg tcatcaagcg tgacatcagg gagggtcagc actcgctccc gaatgccggt
    13201 tacggcagga aaatcttcct caagcatcac ctcgccgttt ttgaaccact catactccag
    13261 cgcggtggcc ggtcccgccc ccacgacagc cgtgagcacc gccgcgccgc cgatggcgac
    13321 cggtgtcagg cgcgtgctgc tgtccaccac caccacctcg gcgggatcac tggtggcctt
    13381 ggtggtggtg gagtttgaaa cctcgcaaac ataaatgccg ccttgcgaca cggctgtctg
    13441 tggaatgaga agcgtgttcg atgaaccgga agccaccgtg gttgttccca gtttccaagt
    13501 gtaagtaagg ggcggacgcc cgccagcctg aacagcgagc atcagcggct ccccggaaag
    13561 gagcgtttga gtcacggttg gctggacttc gaccacaggc gcgggtgcaa gaagggtcag
    13621 ggtcaccggt gtgctggtga acgagttcac atcgttgctg acgaccacgt catagtcacc
    13681 ctccagcatg gcattggcat tcggcaggac cagggtctcc cccgttccac cggaaatcgg
    13741 cgtgccgcct tttctccaga catacgagaa cggagaaccg gtgtcgcgtg gtgtgccgtc
    13801 atgagacacg ctgaaggtga tggtctcgcc cacataggcg cgcgggaagg gggacctgga
    13861 ggcaatggcc tgcgtaattg gatcattcac cgtgagcgcg gcgccgttac tcgtcacttc
    13921 tcccgccgcg ttcttgacca ccacggagta aacgccttcg tcaaactcgg cgactgactc
    13981 caagagaaga gtctgcgagg tagcattctc gatttccacg ccattcagaa gccattggta
    14041 ctcaaagggc gcctggccgg aggcgagcac ggtgaagctg gcgttctgtc cgggattgac
    14101 tgtcaccgga gacggatgca cggcaataaa cggaacggca tccacttcga tcagcactgt
    14161 tgtcggcgcc actgtggtgc cgccgtcatt ggtcacggaa acggagtaaa ctcccttggt
    14221 attgaagttc acctggaatt gataaacagg ctggttggca cccggcacca ccgctccgtt
    14281 cagataccat tgatagagca gcgtgcccgc ccccgtggca gagacgctca actgcacctg
    14341 gtcgccaggc tcgaagaccc ccgtggcggg attgcgggag ggacccgcag tgatcacagg
    14401 agccgggatg atcatcggcg cgctgtccgc ttcgaccacc atgaggatgc cctggctgtt
    14461 gaataggctg acatcccaga ggaagttgcc gccaagctcg aaaagcgtca ggtccgtgcc
    14521 ggtttcgccg ccggtccgca gggaggggcc gacattgatg gcacccgcat aaccgctgcc
    14581 gctgaacaag cggaagatgt cccccgcctt cggcgtgtaa ccatcgttga tgagagtcac
    14641 ctgggtgccc accccccagt tgatggtgcc agtggcatgc agcatgtcat gctggacggg
    14701 tgtcaccaaa tcggccagcg cgttggagaa actgtccgtc gtcacgccat tgcgccaggt
    14761 ggaataaagc gggtcggtgg cgttcatcgc gccagggttg ttgtaggagg gcaggcgaat
    14821 ttgcatgagc gtctgggaac ccgttgagaa aatggcgttc ccatccacgc tcagcgtgcc
    14881 caactccctg cccgcaaggt cgcctgggct gagggtggca cccgccagca gagtcatgcc
    14941 gccctggacg acccctgatc cggcaatcac cccgccggac atcactgttg tacccagggc
    15001 accggtgcgc ccagtgctgc caatgccatt cctgccaact tggaggatgc cgccttcgac
    15061 aacggttgaa ccactgtagt cgttgtgaag ggagagtgtg gcccagccgt caccggcttt
    15121 gacgatgctc agcgcggcgc tgggccggag aatgttcgcc cccggagcga ggaactcgct
    15181 gttgagagtg ccgtccctga agaatgcatc ccaggaagat tcgtagccgg ccggtgtgga
    15241 ctggaaaatc cgcagcgttc cggcggtgga ggcggcgttc tcgatgattt ccgaactgct
    15301 gttgacattg ccggacatgg tgcccgcatc accatggaag gcgccatttc cgccctgggc
    15361 attgagtgag ccgatggtca cgctctggcc gccagcctgg aggatggagt tgaaattcat
    15421 gaccacctca ttggcctcgg tcagcgcctt ggaatgggcc agacgaagaa tggaggtgtt
    15481 gtcctgatta ttgaccaggt ttgaactgat ggtgacatcg ccggtccagg cggagttgtc
    15541 gcctccgaga atggcataac cataggttcg gccgctggtc aggttgttgg cggtggcgtc
    15601 gctgtcgttg gattgtatga gcaggttgcc gctggtgacc gcgccgtcct tgaagctgcc
    15661 gttgtagttc acataggcct gctcgccgcc ggccgcgatt gcaacatcac gcaccagcag
    15721 gatgaggttg tcgttcatcg tgacatcccc gtggaaagtg acgagattgc cgttggaaat
    15781 gttgtcaatg cccaggcgct tggtctgcgc cggattgtaa accacctcaa tgtcatggta
    15841 gatgtcgaac atccagctgc cgctgacact gtccggattc tgcacaagca gttccgctgt
    15901 gaactgccgg tcggcatcgc cgagaatgat ggttttaccc gtgaggtgcc cggagcccaa
    15961 cacgtcggcc tggttcgcgg catagacgct gccctcgcgg atgaccaggc cgcccttcca
    16021 gtccggtgtg ttggtgcgga aatgaagctg gcctgggccg tctttgacaa tgtcacccga
    16081 tccggtgatg tagccggcgc tgttgccaag acggagcacg ccgttgttcg tgacggtgaa
    16141 cgtggtggtc ccgtccacct tgagccatcc gttgaggttc agcgggcgcc tgttgccgat
    16201 gttgaactgg tcgtccagga cgccggagag ggccaggttc gcgttcacat ccagcagacc
    16261 gccgtaggtg aacgtgttgc cgccctcgaa ggtgaaatac tcgtcaatgt tccactcctg
    16321 gccgttgttg gcgttgccca tgatgacctg cacattcgcc ccgttcctta gaatggtgcc
    16381 gtccgcgaag gagcggttcg tgccaaatac cgtcgcagtg cgggcggctg tggcaccatc
    16441 atcacccgtg tccaccatgg tgatcacggt gccctgctgg atgtccataa gcccggaaag
    16501 gctttgattg cctaggaacg tcaaggctcc gacgccagtc ttgatcaaat ctccgcgcaa
    16561 tgcctcctgc gaggtgagtg tggcctgcgc gcccagcaag gccctgccca ccaccagatt
    16621 gccgtcggga ttcgccacat caatgacacc tccgttgccc tccagacgga tgccgcggtt
    16681 ttgcagcgag gctgtggttc cagtccaccg caacgtgccg ccattgagtg tcagacgatc
    16741 gttactgacg tctgtgggat tgaccccaag ggctgagacg cttccaatct ccagcacacc
    16801 accggtgatg atcgtgcgtc catcataagt gttattggcc gagaagcgca cggcccctgt
    16861 tgtgccgact tgcccaggat cggttgtcaa aggtccgttg atcacggtat tgatcgtccc
    16921 ggtgatggtg gactgcacat ccagcactcc ctggccgtat tgatgaaaaa tcagttccgt
    16981 gctgccagat gtgctcaatg tgccgcctgt gatggtcttg ttagcggcac ccgtgttgct
    17041 gctaaccaga atagcgccag gcaaagtgcc gccgcctgag aaggaaagcg ccacacctgg
    17101 attgagtgtc acctggctgt tggccgctgt gtcaaagcgc agagttttga tcgtctcatt
    17161 gccgcctaac gctccggtga atcccgcgcc gttctcggac acatccatgc cggccgtcca
    17221 gcttccaacc gcgttcacaa aatgtccgcc gggcctgttg aactgctgca catcgttgaa
    17281 tctggccgtg gctgcggccg ctgtggccgt agcattcggt gctattgaaa gcacggcagt
    17341 ggtcggagtc ggcacagcga tgacgatggt gccgactaca tatccagttc cagtaaccac
    17401 catgcccacc tgcaaagccc ccgccggact aatgttcgac aaattgacgt tcccgctggt
    17461 cctgtttgca gtaatggcca ctggatcagt cagtgaaacc gtcgcgaaat ccaccgcggt
    17521 tcgcggagtg gtgctgtagg tggcccaggg gatggaggcg tccaatgaaa gcaaggtgtt
    17581 ggccgaattg aagttgagag cgattccccc ggtgcctgtt cccttggccc actcgaccag
    17641 gttcatcgtt gcccccagtg cgcgggtgag cgtgccggca ccctgcatgt tgcccaggct
    17701 gatctgcatg tgctgcccac cacgcccctc ggcgatgacc tcggtggctc caagttgcag
    17761 ttgaagaacg ctgttggctg cgttggtcac ggccagattc gcgacgctgt atgtggacaa
    17821 gttcgactgc gccagcagcg tgccgccggc aaggatggca ttttgctggc ccaccaacgc
    17881 cacattgttg ccggagtaat ccagcgtcag ggtgccgccg ctcaactgcg gattggaggt
    17941 ggcggcggct gtctgattgg tattgcgcag caccacgttt ccgcgcccga ccttgatgac
    18001 tcccatgttg tttggcgcgg tgccaacgtt gccagcaagg ctcatgtcga aagtgaccgt
    18061 tccgccgctc atcgagtaaa ggcgggtggc cacacccgtg gtcgtgagaa tgcctgtgtt
    18121 tcctggcgtg ccgtagatga cattgccact gtcattgaaa ccacccaccc acttcgccgt
    18181 agtgccggag gtgctccagg aagtcatatt gaaattctgc ccgggaacag tgagtgccac
    18241 cacaccaccc acgagagtga acccgtgact ggtggtgccg ccgttgaccg caaacgcgga
    18301 cgacgtggag tcgccgttcg ggatgcggct caaagccgtg ttcgtccaga atcccccgcc
    18361 gtcattgcct gcagccgccg ggtcataggt cacgagcaag ttgccattgc tgagttggat
    18421 gcggcccgca gcgttgtact gccgttcaaa cgtgaaattg gactccaccg aaccagccag
    18481 ggtcaccctg aggacttcgt tctgattggt gcgcgtaccc gccagcgaag gcagcgtgga
    18541 aatcggatcg gaaataggga tgggattgcc gttgatgtcc gccttgtcgc caatctggcc
    18601 gcggaaaagc atgatgttgt tgccggcatc catctgcacg atgcgcgaaa gtgtggcgtc
    18661 aatgttgtgg ctgatggttg tattgccccc gaaaatgttg ccattgatca tgctcgtggc
    18721 cgagttggcg gctgcaattc gcggcagatg caccaccatt ccagccatgt ccacgttgtc
    18781 tatggtgacg ttcgaatacc agcgggtggc atcattttcc gtgcgcatca cctggatgtc
    18841 accagccacc ggtttgacca aaatgtcctc gaaagccgag ggcgagccga cctggatgcc
    18901 gttgccgctg ttcaagccgc cgaggccctc gatcaccact ccgttgccgg ggccgcccat
    18961 gccaagcgcg cttttgccgt tgcgcaaaaa gagcgttcca ttggaaatgc tggtcagccc
    19021 gcggtaggtg ttggcgccgt ccagcaccac ctgggccagg ccggttttga gaagtccggc
    19081 attgccggcg atggcggagc ggacttccag gttgccggtc accacaatgc cgtccgtgaa
    19141 cctcaccatg gcgttctggt tgttgatgac ggcctcgcgg ttgccgaaat tgagcgtgcc
    19201 gccaatgatg aatgtggcca gacttgatga gttgcccgtg gtaaaatagg cgctgctgat
    19261 catgcccgag tcaattttca gtgtgccatc cacatagagc tggatgttgt gagcggccgt
    19321 gtagtcggtg agggtcgtgc cgtcattgat gttgcgcccc tcccggttgt tgtccgccgc
    19381 aggaccgaaa cgcagggagt tgatcgtcac attctcccgc acgcgggtga aggtctccgt
    19441 caaattgatg ttctggccgg cggaaccgtt gagcgagtca atcaggccgt ccgcagcggg
    19501 ggtgaaatac tccgagtccg tgagcgggcg caggatgccg ccgtcaaagg tcatgaaatg
    19561 ggaaccggcc aggaactgct ggttgcgcgc ctgggtcagg taatccgaag cgttggcgtt
    19621 gttgaaaccc agttcgcgaa tgtcctcgcc gagagtgtgc ggtatgatgc cgcccaggag
    19681 accggggatg atttttttgc tcgtcatgcc ggtgccagcc ccgacctggg ccagagagcc
    19741 gatgttgaga agctgcaccc gcactgtgtc ggcgcctgac agcgtcccga aggtggaggt
    19801 ggagtccata ttgcggaatg tgagcacgga gcccggcgcg cgcgctatct cgccgatggt
    19861 cagcgtcatg ctcgtgccgt ccacggtggg catcagatcc aggaagttgc tgcccgaaag
    19921 gacgttcagc cgggcgcctc cggcggtggc cagcgcctcg gtgttgtccg aggatccacc
    19981 catgatgcga agccagccgt tctcgaaatt gatgctggcg gcgttgttga tccggtcgtt
    20041 gtggttgccg cccgcgacgc ccgatgtggc gtccaaaaca ttggagttgt cgagggtgat
    20101 catgccgcgc cgctgcaagt tcacgtttcc agtgcctgac aatctggcat tggctcccgc
    20161 cagggtcagc ccccactcgc cccagccctc ggcatcaccg aaagtctggt aatccactcc
    20221 attcacactc accgtgttgt cctgccagcg gacggcggtc gtgccaaacc tgagcacgtt
    20281 cacatccccg gtgaacgtgt tgttgttggt gaggcgaagc tcgcggaaac ccgtcttggt
    20341 aagaccgccg gctccgacga tctgcccgcc gacgatcagc ttggcctgga tggcaccggt
    20401 catgttcgac tgctcgacga tcgtgttgtt gttcgaggaa gcatccacat ggaagacgtt
    20461 gcgactggcc gggccggtca gtgtgatggt gccgtccagg taggtgcggg agttcggaat
    20521 ggtgcccgca cccgtgtcaa tgtagttgtg accgccgttc agcgctgctg tggcaccatc
    20581 catcatgatg ttcaccgtgt ggtggacatc ccagttgttc cagagattga gacgcgagct
    20641 gagaatggga ccgacaccaa gagatgcgtt tgtcttgtcc gccagtgaag gccccgagta
    20701 ggcgatggtg atgccattgg tgatgttcgc cgcagtcacg cctgcaaagt cggacaccgg
    20761 cccaaactcc ttctcaatgc gcaggatgcc ctcgttaatc accagttcct ggagcggact
    20821 tgtgaaattc gggtcacgaa gagtgacacc cgcgccattc gcgtcgttgg tggtggcgct
    20881 gctgcgcacc gtcagaatcc ggtcgttgcc gtcaatggtg ggacggttgc gggtgaagtt
    20941 gccttccagc actgagccgg tgttggtgtt cgtgtcatag ctcccaagca ccagcctgga
    21001 tgcgttgagg aagccgccgc cgctgatcaa ggcgtccgcg tccatgacga gatgcgagaa
    21061 gacgcccagt cccgtcgaat tgcgcagcgc gccggtgccg ttgacaccgg ttccgctgac
    21121 atggatgacc tcccggaagg catccggcga atccacgaaa ttcagcgcct gtccgcgcaa
    21181 gtcgagagtg gcgccgttga gcaccctggt ctcgttgccc ggtcccgtgc cgccgagggc
    21241 tcgaatgtta cccacccaga gttgcccttc cgacacaaca acctggccga gaagcgcggc
    21301 gttgacattg ggagtcgtga ttcccacggg cgcgccgggc aggatgtctt gaaattggtt
    21361 gccgctgagt gtgaggatgc cattgcccgt cttggtgaag ccgttcgatg agcggatcat
    21421 gttgctgagc gtcagcgtgg aattcgcggc gacctggaag ttcgctcccg ctgaggtgct
    21481 gttgttcagc gtcagcacgc cggaaagggt ggacccggtg ttggtggtgc cgtttggaac
    21541 agccctgatc aatgcgccgt cctcaagaat cagccgtttg gtataggtct gcgaaacgcc
    21601 gaaaaactcc agcgcgcccc cgccgccaat cgtggccagt ccgcgcagat tgccgagggc
    21661 gttggcatcc gtactgaccc tcagagtgcc cccgtcaacc cgcagttccc ccagccagtt
    21721 tgtgttcgtg gagacgagca tcaccgtcga ggtgttggtc ggcgcaagat gaatcaatcc
    21781 cccgccattg agggcgccga gtgtgtaaac cagagatgga tttgccacat tgaacaaaag
    21841 cgtcgctcct tccgacaagc tcacggggtt gcctgcagtg ccgacggcgg tcagatttcc
    21901 aagccgcgcg ttcgcagtgc ctgccgttcc atcgcccacc tgcaggacac cctgctgcag
    21961 attgacgcgc ccggtgttcg tgttcgcacc gccgaggatc atggtgctgg gaccggtctt
    22021 ggtcagtgcc aaaacgcccg tgttattggc gatgacggaa ttcactgtca gcagacccgt
    22081 tccatgatgg tgaaaaacca agtcaaggtt ggcggtcgtg gccgaacggc gaagagtgcc
    22141 gccggaaatg accacattgt ccgtcatgtt cttggtgaca agaatccccc cagccaccat
    22201 ggccagcgcg ctggccgctc cgaggttcaa ggacacagtg cccgatgcgt tcgtattgaa
    22261 gcgcagggtg ttggtcgtgg tcgtggttgc cgtcagattg gttaaagcgg cggtgatgtt
    22321 ggtgtgctgg ccgttggcaa aggtggtgac ataggctgaa aatgcgttca ccgaaccgcc
    22381 gccggtggcc cagtccgtgc cattgactgt gatcgcggca gtgcttccaa tagccagaat
    22441 gttcgccgtg ttcgtggtcg aagttgtcgc gatcccggcg gcgccgaggt ccagcgtgcc
    22501 gtaggcgttg agggtgttgt tggccgtgcg ggtgatcgcc cccatttgaa ggctggcgct
    22561 gcccccgtca cgaacgacac gactcgcgcc attgttgagc agcgtcgagg cgaccacctg
    22621 ggtgaaagcg gcagcgttgc ctctcagcac gagcgtgccg ccgctgtagg gtgcaagatc
    22681 agtctgcgcg gcaactgtgg cgctgggtcc caggccggtc gcaaatccag tggcgctgcc
    22741 cagcgtggcc cagcccagat ggagggcgct gcctgcggcg agtttcgtgt tgttgctcgt
    22801 cgtatagtcg agaatcaacg agcctcccgc cacggttgtc actccagtga aggaattcga
    22861 agcgccgctc aaaatcaaat ccccgcggcc agccttcaaa aaggtgcctg tcccgacagt
    22921 gcctgtaatg gtgccggcga gtcgcaggct ggccccatca tgaaccgtgt tgacgaaact
    22981 tgcgttggtg ccgctcagag tgacgccgcc attgatgcgg ttgacacccg tcaacgctcc
    23041 cacgcctcct agattggtgc cgttggtgac tgcattggcg agcgtcacac caccgcgcaa
    23101 ttgcagtcca gttccggcac cgccattgat cgcgacggtg ccagtgccaa gggcgttgtt
    23161 gtgagtcaca gccagggtgc cgccaccaat cgttgttcct ccggtatgag tgttggctcc
    23221 gttgatgatc caagtgctgg cttcgatctt gttcaaaata gtggcgcctg atccgttgtt
    23281 ggtgatctgc gggctgaatt cattttcggc aatgcgagcg ccgcgcagag tgagcgtctg
    23341 agtggtattg ggagctagaa acgcgatcgc cccggagttg gtgaacttga tcgaaggagc
    23401 aaagtctcca aaagactgcc catccgccca gatgcccccc gctcccacac ccgcgcctag
    23461 ggtgagaaga cggtcggtgg tctggtttgt cgtgccgatc cagcgcagtc caccaatgcc
    23521 tgtgcctgtg tttaagacta gattggaggc ggcatttgag gatgctccga tgccgctggc
    23581 ctcgccgccg tcggcgagaa acagcacgtc aatgcggtgc gcgatgttgc tggcgctgga
    23641 gatgagcgtc gccccctcat aggtgttcaa gttggtgagg gtctgaatgc gcgcagtcgc
    23701 atcaccatcg cgccacagcc cgccttcccc gctgatgact ccgttgtaag tctgcgaggc
    23761 ggcggaaagg agccgcaacg tctggccaga ggaaatctcc accaaagtgc cgagaccacc
    23821 ggccagtccg ccaatggttt ccgaactcaa aacacgcagc gcagccccgc ctgcggaaga
    23881 cccgtccgaa cccgcgttgc tgaggttcac aacggcggcg tcgccgatcg tgctgccgcc
    23941 ggtgatttcc aaagtcccgc gcgtgatggt gacagcgcca gtgaaggtgt tcgcaccggc
    24001 cagacgccag gtgcccgtgc cttccttgat gacggagacg gtgttgccgg tgccgttgga
    24061 gatgctgccg gcaatcgtat tggctccggt gttggaacca tcgagaatcc aactgcggtt
    24121 gccggtggcg ccggaagtaa ggttgccgtt gatcacaatc accttctccc cgttgttcat
    24181 gatctccggg ttgcctgggt ttgtcgtgct gctatagctg ctggtgaagg tgatgttgct
    24241 gcccaaagta gcggtacccg cacctggatc gttaaagatg attgtgccgt tggatccagg
    24301 ttgtcccgtt gctcccagat tgtcaccacc gccagtgaaa gcaaagcgcc gtccgttgaa
    24361 gttgttggtg gctagatcaa tagtgcgggc cacgttcaaa ttccccatca cactgaaagc
    24421 ggtggaaccg ttgccaagat caccagcaaa ttcaagctct ccaacgtcca ctgaaatatc
    24481 cccattgaaa tcgctggaag cattggtcaa cgtccatttg cctgctccat acacatcatt
    24541 atcgccgaca ttcggaccga cgctgatgga tagaagtctg ccgtttctct gaagggcgcc
    24601 gttgatgaca ttttgggtgt tctgtccatg cagcatcaag gttttggcac cgtcggcggt
    24661 gatggtgatg ttgccattca gaacaagtcc gccccgcccg ttgccggcga tgcgcacagt
    24721 tccggaggca ccggagagat tgaagtccct gtcggtgctg tgtcctgtgc cggtgtattc
    24781 aaggcgggta tccaacgaac ttccggccaa accaaagttg atcgcctcgc ccgcacccaa
    24841 cgcactgggt agaaacctgt cagccacgct gttgacacga agtgtggtga tttgtgtccc
    24901 cgatccgatg gtcacattgc cgacataatc actcacgccg aacatgccgt gccacgccgt
    24961 gccacctacc gcgtcaaaat gcatttcacc cccgccggta atggagccaa agttgccgcg
    25021 cgtgatgttg tcgtccactc tcaaagtgac accgttcccg agcaccgtca gagagccttt
    25081 ctgcgccagc aatgaaccga tggtttcgtt gaagttcatc tgaagcgtgg ctccgcgctg
    25141 cacatacatg tccatgtcgt tgttgatgga gtcatcgaaa tcgctgttga tgaccattgt
    25201 gccgcgactg agataaaggg tggaattgct ggtcattgac tgaaccaggg cgtttgtctg
    25261 gtcacgcgcg ggctgcacgg tcaccgtgcc ggtggtgttg aagacgagcg cgttgttgcc
    25321 ggagatcacg ccccagagtt cataattgtt cgtcccctcg aaggcgaaga agccgttgtt
    25381 gatgatgttg atccggttgt tggggccgtt gttcagctcg ccccggatga tcattcgacc
    25441 gccgccctcg gtctggagca cggcgcgctg cgcctgcgtg cctgccgtgt gggaacccgc
    25501 gttgatgatt ccagaaaaaa tcgtggtgcc ggtgccctgg cgcagcaagg tggagttgcg
    25561 cgcgttggag ccgtcgttga atgggttgat gttgaaggtg ttggccagga taacattgcg
    25621 ggccagatgg taacgcatgt tggtgttgcc gccggatgag gactggagcg tcaccgcccc
    25681 agtgcccacg gagccggtgt aggtgtccgt gccgacacgc atttcggcgt tcacattcgc
    25741 cacgccaacc aggccgccgc cgccgatgaa tccaaaattg ccgcccgcca aagtggtgcc
    25801 cgtgtaggtg ttgtccgctg tcagataaag tgtgttgcgc acctggatga aattacccga
    25861 accgctgatg gcatttccca gcaccatgtg tccattgttg gtgttggtga tcacagtgcc
    25921 attgttgaca atgggcgccc tgcctgcggc gccggccacg gcgggggcga tggtgagagg
    25981 gttgttgtcg agcaatggat ccgcccccag aagaagctgc gttcccgcgc caaggctgat
    26041 gccattaaag tgatttgcag cgttgccaag cagcagtctg cccgttccat tgacggtcaa
    26101 ctggctgccc tgcatggcaa aaaccctgcc gttcaagcgc agaggagagg aggcgccgcc
    26161 gagggtgatc gtgctgtttc cgccgagaat caacgagccg ttcaaggtgg aactgccagc
    26221 gcttgggctg gtccgcagcg tcgaaccgcc tttgagcacg atggtctccg gaaccacgac
    26281 gtttgaaaag ctcagagtgg cgccgctgcc gacactggtc aggcccgccg tgctgcccag
    26341 cgcgttgcca gcattgctga tctccagcgt gccggccagc acctgagtcg ggccggtgta
    26401 agtgttgtca ctgttcagcg tcgttattcc cgcgccagcc tgacgcagga tgccagtgcc
    26461 ctcgatgatc tgaggcaggg tgatggagga gcggttgatc accagataac catgattgat
    26521 gagcggctgg ctggcacctc cgagaatgcc accggtattc aattgcagca cgccgccgta
    26581 aattgccgtc tggccgctgt aagtgttcaa tttggtgaga atcagcgtgc cctggccggt
    26641 cttggtcagt cggctcggcc cgccgttgtc gccgatggtg gcattgatcg tgagggttcc
    26701 gcgcgtgttg tgctggtgga cgatcaactc ccgctccaca ccaaaagccc cacccccaaa
    26761 caactcctcg ttgccgcggt tttgatccgt gatggtgata tcgaaagcgc ccaccgccgg
    26821 agtgaccatg atgcctcctg attcaacgat ggtctgctgt gcgtcagacg ccccgcctat
    26881 cgtaagtgtc tgagcgccaa aggtgttaaa ccttagcgac cccgtggcgc ccgagacttt
    26941 cacgtcggtg aacaacgagg tcacatccgt gttcaagccc gcgccaaatg aggaactgta
    27001 actgccatcc gccaggccgc tgatcgccgc cccacccgcc cccggcacag cccagctaaa
    27061 agccggagtc cccgcaacca cggaacgatg cgtggcatag ccaccgagaa tgccgccaag
    27121 accgacctgg ccagtggaag tggtcgtggc ggaaccgccg cgaatgttca gcacgccacc
    27181 cgcagtcgag ttgcggatga gattccccaa attcagaagc gcatccgcct ctcccgtctg
    27241 cgtggcaatg agggaggccc cagcggagat cgtggtgccg ctgctgatga cctccggggc
    27301 cgctccgccg ctcaagtaca aactgccgcc attcaagttg agcgttccag ggatgagatt
    27361 ggtggcgcca ctttcatagt ccaaataaag agccgcgccg ctggagaccc gcgtggcgcc
    27421 cagataactc tggttgctgt tgaccatgag gctgccgcca gtggccagtg tcaaagcccc
    27481 gaagcctgaa atgttgccgg accaggcatg atcaaaaacc gaccccgccg gcccggaggc
    27541 aaggacgagt gttcgtccgg aggtgatctc cacatttccc gcgccgctca gggaccccac
    27601 cgtttcattg tccaatacgg aaagcgtgcc cgcagctccg acgctgacat gcaccgcatc
    27661 acccagcgcg ccgcggctgt cattgaggga gacacgaccg cccagcacat tgagggcgcc
    27721 gcgctgtatc gcgtcattgt tggccagatc ggtggcgcgc agctcccacc cgttggcccc
    27781 cgtggttgag gtgacattga tcgtgccgcg ccctgagaat atgccgttat aaaagcccga
    27841 gttgcctgcc gtcgtgtgga tggtgagtgc gccgccctga agccgcaccg ttttaacccc
    27901 ggccacgttc gccgagttca cagtgccgag cgtctgggag ccgccattga ggtcaaaggt
    27961 gacatcccgc cctgatccga cgttgccggc ggacagcatc aggtgagtcg tggaggacag
    28021 agagccgaaa ccggcggagg cgttcacccc gacacccgaa tggatgccat acgcgccaag
    28081 cctgatggcg ctgtgcgtgt tgttcaccgc gaaggcttca atggtggttg atcccgcgta
    28141 agtgctggtg cccaggactt ccatggtgtt gttgcccacc ttggtgagat tgcccgtgcc
    28201 tgaaatgatg ccgccatacg aatagacttg gttgggtgtg ttgttgtcca gaatgaggtt
    28261 ggtgttcgcg agaatcaatt cgccccccgc ctggttgagg tagttgccaa gcagggcgcc
    28321 tgtggagccg ttcgggaaca gcctcgtcgt gccgaaaccg ccaccagtca ggaatcccac
    28381 cacctcattg ccgcccgaag ccagtgtgag actgaccccg ttcttgttcg acatgacaat
    28441 gttgctgcca agcccctcgc caacaaagcg cccgttgccc aggcccgccg agcctcccac
    28501 gatcatgccg ccggagtcca ccatcaccgt gccgttgaag gcggcgtcat tattgttgat
    28561 ccaggtgaag atgccgttcc cctcctttag caatccagac gccacaccgc cgatgatgct
    28621 gccggtgaaa ctggtcgtcg cattgttgcc gccgaaggcc atcaccgtgt ttgaagtacc
    28681 tcccgtgagc aggatggtcg cggcattgct gccaccggac agcgaaccca cgcgttcgaa
    28741 tggcgcggtg ttgaggacat tggaggcaaa gttcagcgtg ggagcgccgg tggccccgaa
    28801 gacgagggag cctgaattgc tcagcacatc gtgaatgacc gtccgcccgc tgaaattcac
    28861 cccgcccacc tgcacaatgc cgtcattgat ggtaaaagcg cccgtggtgg tgttggtgcc
    28921 gaccaggacc accttgtctg aaccgttttt cgtgaagttg tccgccccgg tcactggatc
    28981 atggatcacg gtagtgtcat caaaagtcag attgacattg gagttgttgc cggtcaccga
    29041 ctggttgatc aaaatctggt ccccgctcag gatttgcacg atgtaggatc ccggcaggat
    29101 gcccgtcccg gtgactggca tgcccggttt caagttcgcc gtggagttct cagagttcag
    29161 ccggatgacc cgcgtgtcgg tgtcaatcgc accggaaacc accaccgccg aggcattcac
    29221 ccagatggac gcgcgacttt gcagtgacca ggagagggtg caggcaaaca tcaacaaagt
    29281 cacagggaac ttcagtcggg tgcgcaggca attcatgaaa atcacggaag gattcctttc
    29341 gggttgggca gatttcatgg ttttgaactt ggcgggttgt attggtcctg gcgtggaagg
    29401 taaagttttt tgctggcaga aatcattccg ggtcgagact gatttcttta aattgttgat
    29461 tatgaatgat tttcctgagt gaaacttctt cggacaggtc ggtttgcaaa gtcttcgaca
    29521 cagggtccag gggattgctc aacttcaaaa tgagggtatt tctgcgattt tttgattttt
    29581 ttctgacaag acgatgtaat cacaaagtca tgtctttctc caagccctaa tttcatgaat
    29641 ccgggttttt cgcgctgggt gcgcatgaat tgccggcact ctggccgcaa ttgtcaaagt
    29701 gtgagaataa gtcgcagtag ctcttgacga cgggacgcaa tttgccaact tcctccgcgt
    29761 ccaagcctca ccccaaccat gatcgtcatg ccattctccg tgccgccacc tctgagtgtg
    29821 cgcctcgaaa gcggattggg cgaatgccgc cagcgccacg ggaactggct tcagcaggag
    29881 gaaaatctcc gcccgctcgc ctggttggtg gcttttgctg gctgcttctt gctgaccggc
    29941 ctggctggaa ttcttcggga ggatgactgg cggatggatt tcaccccagg cagcgcctcc
    30001 gcgcctgccg ggctggacgc cggcatcgtg gaaaccacct tggccgaact ccagtcaacg
    30061 gtcgcggctg agacactgga acccgacagc accgaagttc agttcgaatc gcctccggtt
    30121 gtcgaaatgc cgctcgaact gcaggacctg cccgaattga tcccagccct ggtcacagaa
    30181 gatgtctgca ctgtccccgc agcgccgaag atcgaatccg tcctgacacc tgtggagccg
    30241 ccaaaacccc gccccgtggt caaccgcgcg ccatcacccg ccacgcgccc gccgtcacaa
    30301 acatccgcgc ggcccgcttc tccaggcggc ggcatgggcg gcacaggcat ctccggcagt
    30361 ggcggcagcg gccgctttcc cacacctccc tacccttcct tcgcgcgctc gcgcggcatt
    30421 cagggatccg tcatgctcac gatcatcgtc aatgccacgg gagctgtgga aaacgccatc
    30481 gtgtcgggca gcaccggata ttcggaactc gacagctatg ccgcctcctg ggtgcgccgg
    30541 aactggcgct tcccgcccgg agcggcccgc agtttcaaac tgcctgtcgt tttcaaactt
    30601 cgctaagcgt gatttttgtt ttatacccgc ctgtgatacc cctgccctgc cccgtgctcg
    30661 ccaacgccgt cattgagttc atcgtcaaag gcggccccat catgtatccc atcgtggcgg
    30721 tcgccctctt cgccgtctgc gtgcttgtcg agcgcctgct ttggtggctg aggctcgcca
    30781 cacgccgcag cccccgccag ttggaggaag tttacgccgc gctggaagcc ggggatcccg
    30841 accaggccat cgcgcgctcg gagaaaacca ccgaccccgt cgtgcgcatg atccaccacg
    30901 gtctcaagca cccgcacagt tccatgcagg gcgcgctcga ggtcgccgcc gggcacgaac
    30961 tgcaaaacgc cggacgtttt ctcggcgcca tggacaccat cgtcactctc ggtccccttc
    31021 tcggtcttct cggcaccgtc accgggatca tgggctcctt ttcctccatc ggcggagacg
    31081 agctcgccgt cgaaaaagtc accggcggca tcggcgaggc cctgatcgcc actgcggcag
    31141 gtctcggcat cgccatcgcc accctcatac cgatgaactg gtttcacagc cgcctcgccc
    31201 gcctccaatt cgatctcgaa gccgccgcca acaacgtgct gatcctcgcc gcccggcatg
    31261 gttttgacca gagcgccgcc aggacggcga aatgagaaca gcgaaatacc tgtgaagctg
    31321 cattccccca tcccgcaaaa aaaggcccgc ctggaaatca tccctctcat tgatgtgatg
    31381 ttcttcctgc tggcctcctt catgatggtc agcctcacca tgacaaggca gcagaccatc
    31441 aaggtcaacc tgcccgtcgc cgccgccagc gagcctgatt tcaagcccga catgatcaac
    31501 gtggcggtca gcgccgcggg cgacctttat ctcgacacta ctcccgtcac cctgcccgac
    31561 ctggagcaaa gactcgccga gcgcagggcg cgctctcccg acacccccgt ttacatcagc
    31621 ggcgacgccg acacccgcca tgccgacatg gtcaaggcgc tcgacatcgc gcgccgcgcc
    31681 ggattcagca aagtcgcctt caacgtccgc gccaccggac cggcgccgca gcaaaagtaa
    31741 ggcagcccca tgaagcgctt cctgctctcc cttctcgtgc tcatcaccct ggggctgtgc
    31801 gccgtctgcg ctctgcaatg gcagcgcgag caccggctcc gcgcgcgcat ctccgacctc
    31861 acccgccagc ttgccgagga aaacaaaagg cgcgtcgagt tcgaggaaaa agccatgcgc
    31921 tacgagcagg agatcgcccg cgtcaccacc ctgcgcgcgc agaccgaggc cgccctgctc
    31981 gaagccaccg aaaccctcca gcaaaccgct tcggaccaaa tggcccgcgg acacagcatc
    32041 gccgtccttt ccagcgagct tctccgcgcg cgggcagagt ccgccgcatt gcgcgaactc
    32101 gccagccagg gaaccagcgc catccagcag cgcaacgaaa ccgtcgccgg acaaaacgcc
    32161 gccatcgaaa aggcgaacgc ccggctgcgg gaactcgcca aagagcgcga cgacgccatc
    32221 cagcagctca acgcgcgcac acgcgagttc aacgcgctcg tcgaaaaata caacgcgctc
    32281 gccaagggat cgcgctgacc ctgggttgaa aaacccgcgg ctccgcttcc cggtcctcat
    32341 ggcacgcggg atgcttgaaa gccggacatg gaatccaccc gagtcattca gcttcccgta
    32401 cgctcgcagc cggcccatga accggagttg atcaagctcg tccagtcaca ggggcgcgaa
    32461 aacatctccg gcggcgacgc cgaatcctcc cggcgatttg ccagcgagat tcagtacgtc
    32521 acctggcacg cccgccgcat cgcccccatc ctcggcatgt ccaccctgcg cctcggcatc
    32581 gtcgaagacc gcgagggcca gaccgccttt cacatctcca gcgacggctg gcacggtgtc
    32641 gtcagcggcg gccggcgtcc cttgaggcag ctccgcgaat ccctggcaag aggctgatca
    32701 cggcgcatga acctcgatcc cctcatcgcc gccaccgcca ctctccagag gctggatgga
    32761 gtcgccggaa ttctgctctt caaaggcgcg aacgtcatcc acaagcagat gcccttctcc
    32821 gatgcgcgcg cccgcgagct gcgcgaggca ctcgatgaaa tgctcgccgg ctaccgcgaa
    32881 gtgcggcgga agctccggct cgcgtgtctc gagttcgatg ccgggctgct gctcatcctc
    32941 gtccagggag ggaccgcgct cgtcttcctc ctcaccgccc aggccgaccc cgacctcgtc
    33001 ggcagcgccg gactcgtgct gctggaggac cacgcggaag ccctcgaagc cgcgcccgct
    33061 cattccgaag ccgctccccc ggcggctgac gaagtcgagg aactcatcgt caccagtccg
    33121 cgcaggctcg aacagatcac cgaaaaagcc gcgcccgtcg tcaaccattg gggcgccgtt
    33181 cgcaaggccc tcgaaggact gctcggcaaa gtcatgggcc gcgcccaggc cgccaacctc
    33241 gtggaccggg tgctcgcgga aaaaggcgtg gacgatccct ggcgtctccc ccgcccgcag
    33301 atcgaaaaaa ccgcgctcca tgtcctcgac aacatcccga acatcgccaa acgccgccag
    33361 ctcgccaccg agctgcgcgc cgccctggag gagatccacc ccgatcactg acaagccccg
    33421 ctatccacca tgagtcttcc gcgcctactc cccgccatgc tgctgctcgc cggagccgcg
    33481 ttcagccccc tggcctctca aaccccgtct ccggaaaacc cgcgcatcga ggccaagctc
    33541 gacaccatca tcaccaagct cgacggagtg gccgccgccg tcggcgcgga caaaaccgcg
    33601 cccgaatcac ctccgcccac ggatgaaacc ggcagcgccg cgcaagccag cgtggacctc
    33661 gtctgggtca tcctcgccgg cgcgctcgtt ttcctcatgc aggccggctt cggccttctc
    33721 gaactgggct tctccagggc caaaaacagc atcaacatcg tcatgaaaaa cttcctggac
    33781 ttctccatcg ccgtcgtcgt tttcatgttc ctcggcttcg gcctcatgtt cggcagcagc
    33841 gccgggggct ggatcggcac cgacggcttc tggctctcca acttccccga ggacagcccg
    33901 ctttggggct tttggttctt ccaggtcgtc ttcgccggca ccgcctgcac catcgtctcc
    33961 ggcgccatgg ccgagcgcac caaattcgtc ggctacctcg tctatgcagg cttcctctcc
    34021 ggcgtcatct acccgctcac cggccactgg gcctggggca gcttcggtgg cgcctacgga
    34081 gtcggcggca cgggcggctg gctggagagc ctcggctttg tggacttcgc cggctccacc
    34141 gtcgtgcatg gctgcggcgg cgcctgcgcc ctggccggca ttctcgtcgt cggcgcgcgc
    34201 cacgggcgct tcgccaaaga cggcagcccg cgcatgatcg ccgggcacaa catcccgctc
    34261 gccgccctcg gagtcttcat cctctggttc ggctggttcg gcttcaacgc cggctccacc
    34321 ctcgccgcca gcggcggcat cggccgcatc gtcgtcaaca cagctgtaag ccccgccagc
    34381 ggcgcgctcg ccgccatgct ctccatgtgg ttcgtgcagg gccggccgga cgtgggcatc
    34441 accctcaacg gcgcgctcgg cggcctcgtc ggcatcaccg cctgctgcgc caatatctcc
    34501 cccgcctccg ctttggccgt cggcctcgtc gccggcatcc tcaccaccgt ctccaccatc
    34561 ggcctggagc gcctgcgcct cgacgacgcc gtcggcgccg tgcccgtgca tctcgtcaat
    34621 ggctggtggg gcacgctctg cgtcgcgctc tttgaccaaa aaggcttcga cgcccgccag
    34681 ctcggcatcc aggccctcgg caccttcagc atcaccgcca ccagcttcat cctctgcctg
    34741 gccgtcttca aaaccgtgga cctcctcgtc ggcctgcgcg ccacggatga ggagcaaatc
    34801 aacggcctcg acttcagcga gcactccgcc aacgcctacc ccgacttcca gaccacggag
    34861 caggtgtgag tcggctccct cgcatcaatc accggccttt ctttccaata cagactccac
    34921 ccgttcccgg atcaagacga tgatctccgg gttcatgaac tcatactcat ccgggatgtc
    34981 cagcacctcg atgcgcagtt catgaaacac cttctcaaat cacacagcgc tcactacagg
    35041 tgcagcgaac aaagatgcct atcccggatg gatttctccc acaccgaagc cttgttgatg
    35101 acttccaccg gcatggtggc ttcgatgaga ttatttacgg atggtaacga gattggacct
    35161 gggtcaggtg ttcgaattgc gctggtgctg accaataaaa ccgatgacga tttttttctg
    35221 ctcatcgtcc catgcgaacg ctatgcgaat ggtttcctct ggccgtttac tggtcccgca
    35281 acccagatgc tcgccaatcc aatagtcttt tcggttccat gtgcattgat accaggattc
    35341 gtgcttgccg accgagtgtt tcttctgacc tccgctgaag gaccagcctg ggatcaactc
    35401 gcgcaccgac ttgtcaaggt cagcgcaggg gtgtttaccc gtgcgggcca accagtaggt
    35461 ggttgcgagc cagttaaaag cgcgctcaac ctcatctgga aaacggtaag ggcaatctgc
    35521 gggtttccct ttgttattcc atgagaagac cagtttttcg tgatgatcgc gctccgcatt
    35581 ctccacagct tgtgcgacgg tatctggtgg gagatactga gaaggagtgg tttgatcatt
    35641 ctccacatgt cctttttttc gttgttccaa ggcaaaccga taattctcag cgagggatcg
    35701 ctggcgctcc gtttcttcag cctgggatgc aagctgagat tccaagagtc gaatcttttc
    35761 gctttgctcc tgattatcct cctcaagcat tttcgcccag gcattggttt ttccagaaac
    35821 ctccgcctcg gttttcgctt gctctttggc ttctgcaatg gcctttctac gttcaagttc
    35881 cagaagctga tcccaggtga caccttgatc cggcattttg gatgaggaaa tggcggaaac
    35941 ggcttcgaga atctgagaat ttacctcttt ggtcgcgtcg cgaaacctcg aaaaaaactg
    36001 aggcggccac agcggatgat gataaggaga atccgtgagt ctaaggccag gccagtaaag
    36061 acggactgcg ccatgataac agttgagatg gttgggaata atggaacgaa gcttccaaga
    36121 ggtttctctg ttccgggcac acaacatgag ctagcccggc aaggtgacta ccaactttca
    36181 atggatcaac gagtggctgt tgagtgtcat tgttcacaga aatgaacacc aatggatgcc
    36241 tgcgattttg agagagcagt gctttgacca gcgtgtcaca gtcgtcatct gactccgaga
    36301 gatctaacgg cttggcgtcc agtggataaa tgcttttacc gccaatttga tccagaacat
    36361 ttccacgatt ttcggacggc tcgggcggcg atgaatgggg cgcattatct gactccgatc
    36421 cacgatagaa gatgaacaag tgaagtgggg cggagctcca tctttggctg ccatggtgac
    36481 ttctgttatc cattccaaat cggaatcgtc tgagtctgga tgcctgagcc gaaagcccca
    36541 agcgttcgtg gtatcggttt cgacacggtg cgtctggact ttccaaccac catggaacaa
    36601 gtcccgctca gctaaagtgc ctgcggagac gggctgcaga acaagcggct gcccaagctt
    36661 tttgactcgc tttgggtcca agacccaact ccatgttagt ttcgatatgc gatctgccag
    36721 atcatcctgt gtggacgttt ggttcaaagt gtaagaggca cggtagatag tcttcatttt
    36781 gagaaatcat tccattggac gctggcgctt ctttttttct gctgaaattt gaggagcttt
    36841 ttcctctgac acttctacta cgggcggaga agtaaaaaac gaggccgaag gaataataat
    36901 ggaaccgtgt ggaccgcggg ccgtagcttc gcggagtatc tcccgcgcag cgccaaagag
    36961 cattgatggg ccgttcactt caacggcgtg tttcacaccg tcattgatct ttttcatcac
    37021 cgttgaaacc agtccgacca gttcgatgac gagccggtag gggatatttt ttccttccac
    37081 aggctcgtgg cgaagtgaaa gggtgactgc ccagcgtggg tcgtcaccat cattgagcag
    37141 cctcacgctg gcagaaacgt gcaaatccgc aggatcgagg tccatgggct tgtcacactg
    37201 aaaagacaca ctggcctcca cagtgaggct ggtgacatgg tagtcgagca gttgaatggg
    37261 ggagagtttc atgatccaaa gagggatggg aagttcaatt caccacgttt tgtcgcagga
    37321 cagtcatccc aggctgacgc agcggtgcat gtgaactgaa accggtgact cctaggcgtt
    37381 gtcccggaaa tcagttcccc ccagtcagcg cgtggcgatt gaagaccacg aagctgagcg
    37441 ggagtaaaca atctctcatc aagggcgcgc agcaacgtgt tcaccaactg ggaggggtat
    37501 ccatgcccgt tctcccaacg cgagagtgat ttttttccgc agcccagcaa atcggacaac
    37561 tcgtcttggg agaggttcaa gcgctccctc agggcgcgga tatcctcacc catcaagagg
    37621 cccatgtagc gggcctgcgt cttgtcaatg aggttaaggg actccggagt caagacctcg
    37681 tccccgtcct catccacgcg aacctggact tcaatggtgg cgaccttgcg tgccgcacgg
    37741 ttctccgtag ctggcgaatg gatttcgaag ggtttggtgt gaagtttggt cgtggttttc
    37801 atcgtttgcg gggtttgtga aggcgctgga tggcagtttg aagttcgcca gtcaggtcaa
    37861 tgtgggtgga aaatatcttg atgttaagac ggccctcttt gagaccgatt ttggcgtaga
    37921 gcggtttgac gatgccgagg ggatgagggc atagaaattc ccagacctcc gtgcgaccat
    37981 ccatgaacaa cacttggcgc ccgatgagag caggtgtgga aagagtctga atgagatgtt
    38041 catacgcctt gtggctgatg ccgaacacgc tccagtcaga acgcgcacgt acggtccact
    38101 cgatacagcg ggagtcacca cttttgagaa tgcgaatgac atcctgcctc caagaatcgg
    38161 gaatgggcgt gggagacagt tgcggcatgg ttcaaagtcg gcatgacgaa tcaatggtat
    38221 caattgatac caatgtgtca acagattttt tcaaatccaa catttcggca cctcatttct
    38281 gaagagttca gtgaagtcgc aattcacgct gtcactctga aactctaccc atacaggaag
    38341 gggcgactat agttgtagac gcagttctta gcttcagaag cgaacaaatc cctgcgtctg
    38401 ctcgcccgtg accaagctat cagacaaacc taccggaaga gacttgtgtg accaccggag
    38461 aactcaattc ctcaccagca agctcctccc agtcatctcc tcaggctgct tcacgccgag
    38521 catgtcgagc agcgtcgggg cgatgtcggc gaggatgccg tcgcggacgg tgacgttcgc
    38581 cgcatcggcg gcgacgtaga tgccgtggac gaggttggtg gtgtgggcgg tgtgcgggga
    38641 gccgtcgggg ttgcgcatct gctcgcagtt gccgtggtcg gcggtgatga agagcttgcc
    38701 gccgagttcg aggacttttt ccacgatcat gcgcacgcct aggtcgatgg tctcgcaggc
    38761 gtggacgccc gcttcgacga cgccggtgtg gccaaccatg tcggggttgg cgtagttcat
    38821 gatgacgagg tcgtgatttt ccagtcggcg caggacttcg aaggtgaggt ccggggcgct
    38881 catctgcggt tttttgtcgt aggtgggcac ttccttgggg gagatggcca ggtagcgctc
    38941 ttcaccagga ttcggctctt cgatgccgct gttgaagaag aaggtgacgt gcgggtattt
    39001 ttccgtttcg gccgcgcgaa gctgcgtgag gcctgcggcg gcgatggtct ggccgaggtt
    39061 gttgctgagg ctctcggtgc cgaagatgac gcgggcgccg agggcctcgt aggtggcgtc
    39121 gtattcggtg agcgtgtagt agccgacctg cggatgcacc tcgcggtcga agccttcgaa
    39181 gccagtcttg agaaaggcgt cactgagttg gcgggcgcgg tcggcgcgga aattgaacca
    39241 gaggatgaca tcgccatcgc ggatgcgctg ctcgttggcg ttcgagaaga tcatcggctg
    39301 catgaattca tcgccgcgcg gatctttcgg atacgcggcc tgcacggcgg cgctcggcag
    39361 atcggtgcgc acttcaccgc gaccgagcac gatggcatcc caggcgagct tgttgcgctc
    39421 ccagcgcttg tcgcggtcca tcgcgtagta gcggccgatg acggtggcga tcttcgcgcc
    39481 actcggagcg agatcctttt ccagcttcgc catgtaggcc gcgccgccgg ttggtgaggt
    39541 gtcgcgacca tcggtgatgg catgcaccat caaatcggtg acgcccgcgg ccttcgcggc
    39601 attgcacaaa gcggcgaggt gatcctgatg cgaatgcacg ccgccatcgc tgatgaggcc
    39661 gaggaagtgc aggcgcttgc ctttcgcttt ctcaaaggcc tcggccagca cgggcatttt
    39721 cgcgagttcg ccatcgcgga tgctcttgtt gatgcgggtg aggtcctgat agacgatgcg
    39781 gcccgcgccg aggttcaggt gaccgacttc gctgttgccc atctggcctt ccggcaggcc
    39841 cacgtcctcg ccgctggcgc tgacggtgcc gcgcgggtag gtggcgtaga ggtggtcgtg
    39901 gaagggcgtt cgggcgagca gggtggcgtc gccattggct tgagcttggg ctttgccgcc
    39961 ggggttgatg ccccagccgt cgcggatgat gagaactaca ggttttttca tgggatacgg
    40021 gtccatgcgt caaggcggcg gggccgtcaa gggacgtggc tgcgcgaaca aaacctttcc
    40081 ggctcaagcc acggcgcgta gtctgccccg cagtgagcga tcccattttc attcatgagt
    40141 atcttttgcg cagcggcgcg gctctgaact cggcctcgca gaggcgtgtc tttccaggcg
    40201 cccttatcag agtcggcgcg ggttatggct gcgtgcatcc gtggccggag tttggcgatg
    40261 ctccgctgga tgagcaactg gcggcactgg cccgtggaat caccacaccc ctcacagcac
    40321 tggcgctgaa ctgcgcggcg gcggacgggg ccgcccggga ggcgggcgtc agtctgttcg
    40381 aagggctgga aatcccgcgc agtcattact cgtggagttt cgcgcaagcg acacagccgc
    40441 aattcgaccg cgtgctggag gagggatggc cggccatcaa ggccaagggt tgggcgaact
    40501 acggggaaac cacgcgcttc ctggacaatt gcgcgcgcat gagcggagac tccggccctt
    40561 tgttccgagt ggattttaat gcctgcctgg acgcggacac ttttcagaag ttcatcgaat
    40621 tcatgccgct gcgggtctat cggcggcttg acctggtgga ggatcctttc ccctatgacg
    40681 cggctgcctg ggaagtctgc cgcgtccgct ggggcgtgcg tctggctctc gacaaacgct
    40741 ggcaagaagg ggtgggcggc tttgacgcgg tggtggtcaa accggcgcgg agagactggc
    40801 gcgcggtggc agtccggcat ccagggcatc cgctcatgct cacttcggcg atggatcatc
    40861 ccctggggca gatgttcgcc gcatgggagg cggcgcaagc gcgcgcggca ttgggtgagc
    40921 gcgtggggct gtgcggactt tgcacgcagc atctcttcga gccggacgct ttcctggagc
    40981 gtgtgctcag ccccggcggc gagctgcgtc ccgacaccga tggcggcggg cttggcttcg
    41041 gctccgtgct ggagagcctg ccctggagga ggctggaggc atgagggtgg actggcaaag
    41101 cggcggcaac catgtcgcgc ggaatccggc ggcgccgcat gaaggcagtg gcctcgaagc
    41161 ctgggcgaag gctcggggaa tgagcgggat gtgctttttt caaacctcag gcagcgaggg
    41221 attgcccaaa tgggtggcgc tgcggaagga agccatcctc atctcaggcc aggccgtgaa
    41281 cgcgcacttc gaggcatcct ccaacgaccg ctggctgctg gcactgccgc tgcatcatgt
    41341 aggaggcttt gccattctcg cgcgcgcgca cctggcaggc tgcggagtgg cgcggctgga
    41401 gggaaaatgg agtccgcgag cctttgcgga agcctgcgcc aagaccacgc tcacctcgct
    41461 ggtgccggcc caggtgcatg atttggtgcg tgaaaatctc ccctgccccg ctgccatgcg
    41521 cgctgtggtc gttggcggag gtggcatgac accggaactc gccgctgccg cgcgcgccct
    41581 cggctggcct gtctgccaga gctacgggat gacggaggcc gcctcgcaaa tcgccactca
    41641 ggcatggacg tccgacgggc ctgcgggcgg tgtggacaca ctcgccgtgc ttccgcactg
    41701 gcaaacctcc actgaagctg atggccgcct gcgtttgcgc ggccccgctc tcgccgctgg
    41761 gtatgtcaac cgcgctgcgg gcggggactg gcaatggcag ccgctcgatc cgcaaaacgg
    41821 cctgctgacg cgcgatcatg cgcgcctttg ggatgcggat ggcagacgct ggctaagctt
    41881 catcggacgg gaaaccggct atgtgaaaat tcttggcgaa ctcattcacc tcgcgccatt
    41941 gcaagcgcgg ctggagtctt tggccatcga gctaagctgg ccgatgatgc caatactcgt
    42001 gccgcgaccc gatgctcggg cggagacaga actggtgctc gtgacggagc acggctgccc
    42061 ggatgccggc ccgctgttgg agcgttttca ccagacttgt ccaccctggc tgcgcgtgcg
    42121 ggaaactcgc gtgctcaacg ccatgccacg cagctctcta ggcaaagtgt gcgaggatga
    42181 gttgcggcgg ctcgtggcgg aggaatgagc cgcgcgcggc aagaaatcgg catcactcca
    42241 cgatgcgcgc cttccactcc tgggagttcc ccttcaactc gggggcgtac atggcctcga
    42301 tgcgcgcggg caaggcgctg aagcggccgg ggatctcggc tttgacgcgg tagctgaggt
    42361 tgtggcggcc gcgcggcagg cgctcggcaa agaaggccac gcgctcatcg cgaaactccc
    42421 ggtaggctgc caggccttca taactccagc cgctgcgcac ctcgatgggt tcgaaaccgg
    42481 cgggcttcat gtcctcgatg agcacatact catagtcgtt cttgctctcg atggagagct
    42541 ccacctcaat gaggtcgccg ctgcggattt cggcgtcgct ggcgagggcc tcgcggcggt
    42601 atttcagtcc gcgctgggtg acgacctggc cgcgcgcccc ggccacctgc tggtcgggct
    42661 tttcctcgat gaggcggtaa aagcggcgct cggctttgac ctccagccca gcggcgggga
    42721 tcatgtcttc ctgggtgaag acggtgaggt aggcgctgac atagaggggg gaggcgccga
    42781 gcttgcgcag ttgcagcttg tgcgcgccgg tggtgagggc ctcgccttcg agcacaaagc
    42841 ggttgtcgaa ggtgaagagc gtgtcgggcg tgatctcgac gcgcttttgc ggcacgccgt
    42901 cgagcaggat ctccacggtc tgcgcgggcg cggactcgcc gctggctttg gcgaactcgg
    42961 ccagggcctc gatggcggcg gcggtgtcct tggtgctggt ccaccaggtg ccgttgcggc
    43021 ggttgttgac gatatacttg gcgagacggg cggcggtttc acctttgggt tcggctttga
    43081 ccagcaggcg gagaaaggcg gcctgggtct cgatgcggtc ctcataccag aaccaccacc
    43141 aaccgccctc gggcaggtcg agccaggcgg tctggttttc atcgtcctgc ttgaggaact
    43201 ggcgcaagtt gcgcaggcac atgtcgcgcc gttcgcgctc gccggtggcg tcgcaggcga
    43261 gtccgagcaa ggcgaggttg tagcgggaaa ggccggcgcg ttggtcataa agccgcgcgc
    43321 gcatggcggg gtcgccgccg gagtattcga cgagcacact gtggatcagc gcatcgaggt
    43381 tgtcgggcgc gcttttgtgc tcgcggtctt tttggggcag gccaaggcgg cgcagttgtt
    43441 cggcctcgtg ccgtgtgagc cactcgacgc cgttgcggat gaggccctcg ttgagatcga
    43501 acccggcacg ctcggcggcc ttcatcccgt gcaggacgag cgcggtgatg tgcgcggagg
    43561 attcgcgccc gccggggaac cagccccagc cgccgtcggc attgcgcatg gcgagcagtc
    43621 gggagacgcc ggtgcgggcc attttttcca cctcggcttc agagaagacc gcctcgcgca
    43681 ggcgcttctg ctcaccggga ggcatgggcg cctgggcagc ggcatccttg agacgctcgc
    43741 gcaccttttt caaatccacg ccgaggtcac gaatggcctt cagcgtgatg acggtgggca
    43801 cgaagcggtt cagggtctgc tcggtgcagc cgtaggggta ggaggccagg tagggcagcg
    43861 cgtccacgag cgtcatggcc agcgtggggg aatagacgac ctccagccgc gactggcgcg
    43921 ggcggcgctc ggcgggcacg ttcagcgtga ggctgccgct ggcctggtcg gggcgcaggg
    43981 ccaggctcca ggagtcggtc ttcaacatgc cgtggatgta agccgggaag gtcatctcca
    44041 tggcgtcgct gtccttctgc gccagggcct tcacgcgcag cctagcctcg ccctcgccgg
    44101 tgactttcac gcgccaatcg aagcgatgct cgccatgcgc gggcacctgc acggaggcgg
    44161 tctgcggcgc gacggcgaac tccagcacgc cgccctccag ttcgagcacg ccgcgcacat
    44221 cctgagcggt gtcgagctcg ttgtggacat tggcagagat gacgacctcg tctttttcca
    44281 caaagaaacg cggcgcttgc aggcgcacca tgaagttctt gcgcgtgatg acctcgacgc
    44341 tggcctcacc cacctgcgtc tgcggcccca tgacccagga gcgcagcttc caggtggtga
    44401 ggttttccgg caggttgaag gccagctcgc ccatgccctc ggcgtccgtg gtgaaatccg
    44461 ccacccacac ggcgctgtcg gccaggttcg tgcggatcat cggctggggg ccttcttcgg
    44521 gggcaccgcc tccgcctgga gcaggcatgg cggcggcctt atccatcggg gcggcttcgg
    44581 cagcggtact catgacggcc atcggagcag gagctgccat gggcataggc atccctcttc
    44641 gcatcgccat gcctccaaca gcccccgaat acgtgttttt ggctgtttgc ctgtccatgt
    44701 tgccaaaatc atccgccacc tccccaccaa agatacccag cgcctccatc cacttctctc
    44761 catccttcag caggcccgtt tccaaggcgt gaagcgtgtc gcgcacctgt ggatagtggc
    44821 tgcgtttcca gccccagaag aaggggcgaa tgtcttcctg gttgctgccg ccggagatgt
    44881 actccagcgc tttgtcatag gcggtgagca ccacgcggcc ggtaaagggc ttgccatcgg
    44941 cgtccttgac ccgcactttg accttcgatc cctggccggg cagatacgtc tccgcatccg
    45001 gctccagttc cacggtggcg atgcgtttct ttggcggcac aaggatctgc cgtgtcacct
    45061 gatgcacgcg cgcgccgttg acggtgaagg cgtcgaggaa aaagttcggc tggtccgcct
    45121 cggtgatctt gaagcggtgc gtggtgctct tgccctccag atgcagccac acgggatcgg
    45181 gatacagccc attctgcacg cgcaaaaaca gcgccacggt gctgcctggg cggttcgtgt
    45241 tcactgttac ctccacctcg tcgcccggcg cgtattcctc gcgctccacg agcagctcca
    45301 gatcgtcaaa gcgaaagtct ttgccctcat caaaacccgc gccgcgcacg gtgaggaagg
    45361 tcacaccctc gatctcgtgc cccgccttgt ccttgagctt cacagacacg cgatactgcc
    45421 cgccgcgcgc cagagtggtc ttcacgttcc gcgtgaagct cgactcatcc agcttgatgt
    45481 ccgccgtatg cacggcctcc tccgcaggct tgccgtcctg gtcataggtc acacggtaaa
    45541 gcaccagcgt gccctccgcc gccacctcgc gcccgtcgag cgtgcgcgca tggacgctga
    45601 actccgcgtc ctgtcccgcc gtataatagc cgcgatccgt cgagacatag acttcaaaag
    45661 gacgccgcgc ggccagcacg ctgcttttgc caaagatggc gcggcggctt tggtcggtca
    45721 cctcggcctc gatctcgtag cggtggtcct cgttgccatg caactccttc gccagcgcgg
    45781 tgtcaatttt cacagtgaag gtgccgtcgg cattcagcgc cgcctcgccc tcggccacca
    45841 gctcaggggg atcatcctgc cagcgaatcc acggccagcg cggaatgcaa aagcaccagc
    45901 ttcgcgcgcc gggataccag tcataatagg tggcgcgcca gccatagccg gagccgaaga
    45961 gccagtccca gcgcccggcg gggaaccagc ggctggtgtg ggcgctgcgc tggaccttgt
    46021 acttcactgt gccctgcttc accggcccgc cgaagtagta gtccgccttc accttcacct
    46081 cgaagctgtc gcccaaagcc acgggcgtga cgggcgcgtc caccttcacc tcgaactcgg
    46141 gctttttgta ttcctcaaca cggaaggaat ggctgccaag gtggaccaca ccgcgcagtg
    46201 cccgggtatg ctcaaactgc acgttataga cgccgagcgc ggcctcctca ccgagcgtga
    46261 gcgcatccga caccgcgccg gagtcatccg ctttgtaagt tttttccacc agcttctcgc
    46321 cgcgcgggtc aaggatcgtg actttgatct ggctgcccgc gaaggcgttc gtgtccagct
    46381 tcgaatcata gcccaccatg cgcgcccaag ccttccactt cacctcctgg ccgggacgat
    46441 acacgggccg gtcggtgatg gcatagagct tggcctgttc gttgaagcgc tcatacgagc
    46501 cgccgaaata aaagtgcgcg aagcccagga aggcgagtcc gccagccttg gtatccgcgc
    46561 ggatgagcca ctgataattt tccatctgcg cgcgcttcac ctggatctgc ccgtgttcgt
    46621 ccgggctagc ctggaagtct ttgaagttgt aggtcatgcg cggcttgttt agcaggccgg
    46681 ggcgcgccca ctctgaacga taacccatga atttcacccg cgcgtcccgc accggctcgc
    46741 ccgtgacggc atcggcgatg taatacaggc cgccatccgg ctggcgcaca tgcacaatgt
    46801 tcaggctctc cagccagagc agcgcccgcg ctttgccgcc gccttcaaac gcgccctcca
    46861 ccagccatgc gcccgcctct ttcaaaggcg ttggcacatc cacccggcgg tcccaatgat
    46921 tcgcgcgcgg ctccaggccc tgcgtccagg tgtggctcac cggggccagg tatttgtcac
    46981 cgccctcttc gagaagacgc cgcccgatca tgccgaggtt cgtcttctgc caatcccgat
    47041 cctgcggctc gccgcgcagg taagcctccg tgtcatccag cagccgcccc acatccacac
    47101 ggcgcgcgct tagctcgatc ttgcgcgcat tgcgaaacac gagcgacaac ttcgcctccg
    47161 tgcccgcagg ttgcggcggc agggggtcaa aacgacccaa gtttccggtg atctggtcga
    47221 gccgggactg tagctgtgct ttttgattcg tgtccttttc cagtgcgatg gcctgccgca
    47281 gcgcctccga agctcgggga tactggcggc ggtcgttcat ttcctcggcg atctgcgacc
    47341 acgcacccac tctcaaataa tcaggcgcgt ccgcgctctc cgccaccgcg cggagaaggt
    47401 ggaggaacgc ccattcggcg ggcagttcga aacgcttcgc gccagtggtc agccgcgcgg
    47461 tggtttcgcc gtccttgagc gtgtgcaggc tggcaatgcc gccacgtgtc tcagactcgt
    47521 cgctttcact gctgtcgaaa cgaatgccgt aataagccag cgtgttcacc gagaaccatt
    47581 gccttgccat caccgcgcgg gtttgcctgt gctgccagac actggcaggc tccatccgct
    47641 ctctctcctc catgatccag agcaggcgct ctccatcact ggcggcggac tcccagcttt
    47701 gcgcggcttt gaagaacacc ggatgcccct ccgcatccac cggatagccg ccaatggagg
    47761 agtccagtcc gctttggtca tcatagtcag gcagttcgga gagatccgtc agcacctgca
    47821 aggaccaggc ctgatgaaaa taattccgcc cgtggcgcaa ggcgctctcc aacccgtgca
    47881 ggacatccaa acgaaccggc gaggtggcat ccgcaggcag cgctttccac gcggcctcca
    47941 gcagctgcaa ggctcgcgtc cggtccctga tctggctctg cacatgccgc ccgccgccac
    48001 gataaccgcc acgccggtat tcgccatcca agacctcgcc atacgagggc agtctcagca
    48061 ctcgtatgcc cgcattcaga agcacccgcc agctttgagg gaaacgcgcc gcggcatcct
    48121 cgatcacctt ttcctgctcg acatgtgctc ccagagagga aagcgactcg aacgtgagat
    48181 ccagcacctc ggcggcaagg ccggcgtcct gagtggaagc caggacctgc ttgccgagat
    48241 cggcggcctc gcggtggttg ttctgcgcgg ccaggtcgcg gattttggcg ggcggatcac
    48301 cgctttgctg ggcatgaagt gtggcggaca tggcagcggc gagacaaagg cggaggaggt
    48361 ttttcatgac cgtggcggag ttttaccaaa gagcgtgacg tgtgacaaac aaatgtcttg
    48421 gcacggacgc gctgaaagac gcgttcgttt gcaaagcgtg tgtaaaatca cgcccatggt
    48481 tttgccgcag caaaagccgc gcgaagagtc agttcagcgg ctttaattcc ggcagtgtga
    48541 acttgccgtc cttgcggatg accctgccgt cgaagcgaat ctcgccgccg ccgtactccg
    48601 gacgctggat gcagacgaga tcccagtgga cctggctgcg gttgccgttg tcggcgattc
    48661 cttcgtaggc ctggccggga gtgaagtgga aacttccagc gatcttttca tcaaacagaa
    48721 tgtcccgcat cggctcgcga atctcgcggt tgaagccgat ggcgaactcc ccgatgtaac
    48781 gcgccccctc gtcgctgtcg aaaatcttgt tgatggcctc ggtgttgttg gctgcggcgt
    48841 tgacgatttt gcccttggaa aactccagcc gcacggagtc gaaggcgatg ccctggtaga
    48901 tcgtcggagc attgtaactg atgacgccct ccacactgtc cctcactggg gcgctgaagc
    48961 actccccgtc cgggatgttg tgacgccctc cgcaagcgat ggccttgagg cccttgaggc
    49021 tgaatttcaa atcggtgccc ggtccggtga tgtgaacctc ctttgtcttg tccatgagct
    49081 ttttcagggc gttcatggca gggatgagcg cggcgtagtc gagcaggcag accttgaagt
    49141 aaaaatcctc gaaagcctcc gtgctcatgc ccgcctgctg ggccatcgcg gcagtgggcc
    49201 agcgcagcac acaccagcgc gtgttgttga cgcgctcgtt ctgcagcgga cgcaggcgcg
    49261 ccatgaccat cttcatcttg tcggcgggca catcgctgtt ctcggtgatg ttgtggctgc
    49321 cgcgcacggc gatgtagcag tccatttttt tcatcagatc caggtcgctc ccgccgatga
    49381 tctcatactg ggagtcgtcc gcatggatca tcatctcgcg cgtgacgcgg gtgtcgtgca
    49441 gacgcacgac ggggcgggcc tttgcagcca ccacggcgcg gatcagggcc agcggaacgc
    49501 agttcggcac atcgtagcag tcgagccaga caaggtcgcc ctttttcacc tgggtggagt
    49561 attggacaag ctggcgggcg agttggtcaa cgcggggatc gtgcatggca ggcgagctat
    49621 agacgcgcgc cgctggcgca tcaagcgcgg caaacgggga gccggacgaa aagcgagtcc
    49681 agcgaaacat ttcaaacgtt cgtttgacag atcccccgga ttcaaacacc tgtttgaaaa
    49741 tgtctctccc atcttccgaa gactccggca cgcgcgagcg catcctcctg aaggctgcgg
    49801 agctcttcgc ccagcgcggc ttcgagtccg tgtccctgcg cgacctgacc caggccgccg
    49861 aggtgaacct ggcggcggtg aactaccact tcggctcgaa ggaggcgctc atcgaggagg
    49921 tggtgacgcg cgtcatgcgc ccgatgaacg acgagcgcct gcgcctgctg gacgccgccg
    49981 aggccgccgc gcatcccgcc cccgcgcccg ccgagcaggt ggcggacgcg tttctccgcc
    50041 ccatcatgct catggtgcgg gagtcgccgc tctcggagat gcttttcttc aagctcatgg
    50101 gccgctgcct ggcggaccgc acgaatctga tccccgccgc cgccatgccg cacttcgagc
    50161 agatggccct gcgctttgcc aaagccctgc gccgctccaa ccccgcgctc acccatgccg
    50221 aggcctgctg gcggctgaac ttcaccgcag gcgcgctcat cagcaccctg gcccatgcgg
    50281 acatcgccat gcgttttgcc ggagagctgg agaatccccc cgacactgaa acggtgctgc
    50341 gcaggctggt ggcctattgc acgggcggca tggagtccgc gccagccggc aggaagcgcg
    50401 cgcgcggccc gcgcccggtc aaggccgccg tgctcggtct cggcctcgcc tttgtgctca
    50461 gcgcctgcgg cagcctctcg ccgaagcagc ggatggacga gttggacctc gaagtgcccg
    50521 gcacctgggc cgcgagcaag gaggcgcgct ccggcgtgga cgaggtgtgg atcaaacgct
    50581 tcaaagaccc gcgcctcgag gcgctggtgg acgaggcgct gcaaaacaac cccgacctgc
    50641 gcgccgccgc cgcccgcgtg gaacgggcgc gtggccagac gcggctggcc ggcgtggcgg
    50701 gcaaccccac cgccgagctg aaattcgacc cc
//
LOCUS       DAOMYJ010000006        50459 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Prosthecobacter sp. isolate AMR_MDS_5629
            NODE_266_length_50459_cov_21.467463, whole genome shotgun sequence.
ACCESSION   DAOMYJ010000006 DAOMYJ010000000
VERSION     DAOMYJ010000006.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867850
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prosthecobacter sp. (wastewater metagenome)
  ORGANISM  Prosthecobacter sp.
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Verrucomicrobiaceae; Prosthecobacter.
REFERENCE   1  (bases 1 to 50459)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 50459)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.30
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:34:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,742
            CDSs (total)                      :: 4,684
            Genes (coding)                    :: 4,676
            CDSs (with protein)               :: 4,676
            Genes (RNA)                       :: 58
            tRNAs                             :: 55
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..50459
                     /organism="Prosthecobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_266_length_50459_cov_21.467463"
                     /isolate="AMR_MDS_5629"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1965333"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1547)
                     /locus_tag="PLP58_01160"
     CDS             complement(<1..1547)
                     /locus_tag="PLP58_01160"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase"
                     /protein_id="HRJ07159.1"
                     /translation="MKTILTALLLFLAASLPAADKPNVVLFLVDDMGWMDSGIYGSKY
                     YETPNIDRFAARATRFTQAYANPLCSPTRASIMTGKHPARHGILTASGHQPPQPPGFQ
                     FMPDSAPANRPLISPVSKNYLDPAERTLAEALRDEGYATAHIGKWHLGLTPEFRPEAQ
                     GFDVAWHCAPDPGPPGEYFSPYGVVPEGHPTARSKVGTITDGPPGEYIVDRQAAEAVK
                     FLRAHKERPFFLNLWCYGVHGPWGHKPEFTAEFAKKADPRGAQRNPIMASMLRSVDEC
                     FGRILDELETLALTGNTLVIFYSDNGGNVHSNIPGTGKTAAAEKHKGGFLADWRKWAG
                     EEPPTNNHPLREGKGKLYEGGTRVPMMWALPGRIREGAVTPALAGHIDLYPTVLDLLG
                     LEKKPGQVMDGVSLVPVLTGAATAVREEFFNFFPSQQNGGGVTVVRGAHKLIRWFAPG
                     VPRELYDLEKDISETTNMAERMPDKARELDARIDAFLKETGALFPRPNPAYQPRPDGA
                     AAAAARPL"
     gene            complement(1609..2460)
                     /locus_tag="PLP58_01165"
     CDS             complement(1609..2460)
                     /locus_tag="PLP58_01165"
                     /inference="COORDINATES: protein motif:HMM:NF012998.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004664 - prephenate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0009094 - L-phenylalanine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase domain-containing
                     protein"
                     /protein_id="HRJ07160.1"
                     /translation="MPPPADSPTPALVACLGPEGSFSHLLAGMRFPNTPVRLMAGIGE
                     VFDFLADAPEAQGIVPIENSSGGFIIDTVDRLIDERCTLRIIEELTLDVRLALLGRAG
                     AEVASIHSHAMPFFHCEEWLAANYPRARRVVEASTAKAAEKAASLQSAAAIGPRQNAA
                     LHALDILHFPIAGEVPNITQFYLLARAGNAPGPANNRTALVVELPDRPGSLCRFLTPL
                     SEAAINLKRLESRPIRGQPNKYRFYIEIEGSPAEAHVRAALDQARADGAHLRSLGSYP
                     SGRRFES"
     gene            complement(2615..3007)
                     /gene="rpsI"
                     /locus_tag="PLP58_01170"
     CDS             complement(2615..3007)
                     /gene="rpsI"
                     /locus_tag="PLP58_01170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002942223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HRJ07161.1"
                     /translation="MSKPLTSATGRRKTAVVRVHILDGSGSITINGRDFEEYFPTVAL
                     QNQILTPLALTNTRQQYDFNINARGGGITGQVGAVRMGISRALITVNPELRGTLKKAG
                     LLTRDSRAKERKKPGRPGARKRFQFSKR"
     gene            complement(3036..3470)
                     /gene="rplM"
                     /locus_tag="PLP58_01175"
     CDS             complement(3036..3470)
                     /gene="rplM"
                     /locus_tag="PLP58_01175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018968961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HRJ07162.1"
                     /translation="MKTFSAKAQDVKRQWWVIDAKDKVLGQVAVAAANLIRGKTRPTF
                     TPHVDTGDFVVVINADKVVLTGKKEQQKIYTSKRGGYIGNQKVENVEKLRARKPEELV
                     RRAVKGMVPHNRLGNAIVTKLKVYAGAEHPHATQNPQPFSLA"
     gene            3739..5166
                     /locus_tag="PLP58_01180"
     CDS             3739..5166
                     /locus_tag="PLP58_01180"
                     /inference="COORDINATES: protein motif:HMM:NF012234.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HRJ07163.1"
                     /translation="MSSLQAIRDALQQSPDNPSLLLLYGRACLEQLHLDEARGAFERV
                     LEIDLAHADAQIGIARVHYLEGDTSGAAVRAERVLQLEPGNAQAHLLLSRVYLSENDR
                     ARAVEHFDLAAQADGSVHDPALENELGRTARDARRSSPAPAMPELPGVPDSGEENEAA
                     QGFFDDSFDEPPYDWRPETFFAPGDPQRFSTTFADVGGMDELKEEIRLKIIYPLQYPD
                     LYKAYGRSTGGGILIYGPPGCGKTLLLRAVAGEVPCNYLSVGLHEIFDPYFGGTEGNL
                     HQIFETARANAPCVLVFDDVDSLAQDRRSVRESQMRNLVNQFLHEMDGLRDNQRVLVI
                     GATNQPWQLDPAFRRPGRFDQAIFVRPPDDAARAQIIVLQARDKPISNLDVPALVEAT
                     AGFSGADIEWVFGRAAEMTLSAAIHSGHAVPIPMELLLTVARSHTPSTQNWFDGMRDH
                     ATQAAPDGFFNEVRKYLNAPSTKER"
     gene            5182..6267
                     /gene="mtnA"
                     /locus_tag="PLP58_01185"
     CDS             5182..6267
                     /gene="mtnA"
                     /locus_tag="PLP58_01185"
                     /EC_number="5.3.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009965737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046523 -
                     S-methyl-5-thioribose-1-phosphate isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0019509 - L-methionine salvage from
                     methylthioadenosine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-methyl-5-thioribose-1-phosphate isomerase"
                     /protein_id="HRJ07164.1"
                     /translation="MRVAGEHHRTVRLAGDVVRVIDQRLLPWRFEFLDLASVDAAAHA
                     IREMAVRGAGCIGATAGFAMTLAAREADSWRELEKLAGLLKATRPTAVNLAWAVDRQI
                     EAIREAGFDRRVETAVMTAQAIADEDAAACEAIGRHGLDIIRAIAARNPGRPVNILTH
                     CNAGWLAFVDTGSATAPVYAAHAAGIPVHVWVDETRPRNQGARLTAWELAQEGVPHTV
                     IADNTGGHLMQHALVDMVITGADRVSRAGDVANKIGTYLKALAARDNGVPFYVALPSS
                     TLDWSLRDGVAETPIEQRGEGEVTHLEGVTSAGVVEKVRVVPEGSPAANYAFDVTPAR
                     LVTGLITERGVCAAEEGAIRALHGKTA"
     gene            6301..8361
                     /locus_tag="PLP58_01190"
     CDS             6301..8361
                     /locus_tag="PLP58_01190"
                     /inference="COORDINATES: protein motif:HMM:NF012371.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HRJ07165.1"
                     /translation="MHIRAVTGFLSILLLSAAPHLRAQYPGKTWESLSDADAAAAGWS
                     REKLASAREFSATLQTEAVMIVIRGRVLDSWGAVDRKFNIHSIRKSFLSAMYGIQVEA
                     GKIRLDATMASLGIDDNQPSLTEVEKGATVRQLLQARSGVYHPALYETASMKARRPAR
                     HSHSPGAFWYYNNWDFNALGTIYEQHCGARIHEDFSRLIAAPIGMEDYIPADGSYVTG
                     ADSIHPAYPFRMTARDMARFGLLFLRGGKWQDRQVIPAGWVVESTASYSDAGAAGGYG
                     YLWWIAQSGVHLGGVTLPGGSYSARGAGGHKILVIPALDLVIVHRVNTDIEGRQVSSA
                     DFGALVRRILDAYAPPPVSGGVPEALDALMPVLMSRHHVPGAAVLGIENGRVAWEKYL
                     GLREAGKTARVDAATVFEAASMTKPLAAYRALQLVEQGSLDLDRPLAAYLPAPYLQDE
                     PLHEKITARMVLQHSGGFPNWRPKGAALKVMHEPGAAHLYSGEGFLFLQRVIEHITGR
                     DYEQDMQAALLRPLGMKDSSHVWQERFASSAAAGHDGKGAPKPDRRLYTKPNAAYSLY
                     TTARDYAAFVIEMMKADRSAPHSLKAETLRAMLTPAGPPASRECLTRRGAKAEGVVQY
                     GLGWAVEPCASGPRIRHSGSNGTGFRSHVEFDPVAGHGLLIFTNSTSGDAFWRELLGF
                     IGRP"
     gene            8433..9008
                     /locus_tag="PLP58_01195"
     CDS             8433..9008
                     /locus_tag="PLP58_01195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ07166.1"
                     /translation="MRWSLLESAHAASWAQAGIPDEGAVRVQDGELALQTGQPMTGAR
                     FTAWESAGLPGAYYAIEYEAMRVEGRDIFGMVTFPVSSHNAHATFVLGGWGGTVTGIS
                     SIDYSDAGENTTRGEQRFENNRWYRVRVEVRPDDLRAWVDGLIVVNASIKGRKVGLRP
                     GYIDHCLPFGFATWGTSARIRSVVVEKLRAH"
     gene            complement(9041..10510)
                     /locus_tag="PLP58_01200"
     CDS             complement(9041..10510)
                     /locus_tag="PLP58_01200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009960829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1501 domain-containing protein"
                     /protein_id="HRJ07167.1"
                     /translation="MDPFFQHHLLQTRRRFFEGMGLRAGGIALASMMGRDLASAAPPA
                     VGAVHPPLPGLPHFAPKAKRLIYLHMNGAPSQLDLWDYKPQLREHYDKDLPESIRKGQ
                     RITTMTSGQARLPVAPSMFQFQQHGHSGMWVSELMPHTAKIVDEIALMKSVHTSAINH
                     DPACTFVMTGSEVPGKASLGSWLAYGLGSESNDLPAFVVFTPSFPKESQAQALFTRMW
                     SSGFLPTQYTGVALRGQGDPVLFVKNPPGVQQGDRRAMLDALNRLNHINHSRIGDPDI
                     QTRIANYEMAFRMQSSVPELTDLGKESKATLEMYGEDVNRPGSYAHSAIMARRLIERG
                     TRVVQILHRGWDQHNNLPKLLRGQIKDTDQATAALVMDLKQRGLLQDTLVVWGGEFGR
                     TVYSQGTLTKDNYGRDHHPRNFCMWMAGGGVKGGIVHGETDDFSYNIVKDPVHINDLN
                     ATILHLMGIEHERFSFKFQGLDQRLTGVEPHHPVKAVLA"
     gene            10612..10887
                     /locus_tag="PLP58_01205"
     CDS             10612..10887
                     /locus_tag="PLP58_01205"
                     /inference="COORDINATES: protein motif:HMM:NF021474.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapB family
                     antitoxin"
                     /protein_id="HRJ07168.1"
                     /translation="MKMTMHINEDVLADVMEITGASSKTAAVERALTEMVRKHRLKCV
                     LREGMGMTPEEIRESYDFSHADLPETPLSFERKPVKYSAKNAGKSSR"
     gene            10865..11260
                     /locus_tag="PLP58_01210"
     CDS             10865..11260
                     /locus_tag="PLP58_01210"
                     /inference="COORDINATES: protein motif:HMM:NF013970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain-containing protein"
                     /protein_id="HRJ07169.1"
                     /translation="MQGSLLADSSFYIGRLREGKDPLAELAAISEEWEVVTCGVVVVE
                     VCRGFKNERMRERFERAFSTMIMIPTTPKIWHRAMELAWKLDREGKGMQVTDLVIATC
                     ALQVEATVLTLDSDFLRVPSLDVVAEPPL"
     gene            11306..12499
                     /locus_tag="PLP58_01215"
     CDS             11306..12499
                     /locus_tag="PLP58_01215"
                     /inference="COORDINATES: protein motif:HMM:NF018178.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1080 domain-containing protein"
                     /protein_id="HRJ07170.1"
                     /translation="MRTAFAAATCLVLLASCQPADESDGFVPLFNANDLQGWVNANCA
                     PETWTIKNGIISCTGKPVGALRTERQYENFILEIEWRHLSEGGNSGVFVWGGPVNAPG
                     VPFLRGIEVQVLDHGYMKKADPKKPKWFTTHGDVFPIHGATMEPHGEHNGMRSFPSEE
                     RSMPSPEWNHYRIEANNGRITLAVNGKVVSGGDNCNPSKGYLALESEGAPVEFRNARI
                     KELPSSNPPAEMISPLDEGWKCLYTGTDFRGWKVPAAGGEKWESADWQIKLKPGQTGS
                     ALWTEQEYGDCEVICDVQLPKDTDLSKPAAGLCLRGHSHPVVMLGQGEAPVVLGADQI
                     SPGKWYRIKASLQGDKLKVLVTETQEANPRSFEATVDRNPRGSIGLADLAQPVVYGNV
                     NVREL"
     gene            12567..13364
                     /locus_tag="PLP58_01220"
     CDS             12567..13364
                     /locus_tag="PLP58_01220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ07171.1"
                     /translation="MRRAFSLICQTALSIPLFNKVMTTVAYEQKRLLRRRVEERLRAA
                     GLYPDVVMQGPLKGTRFPPGYASCRFEKIIGSYEAETHAWLDELIARGGFTSIVNIGA
                     AEGSYASGLARHFPEIPIFAFEANEALHPAIHEMAALNDVEGRLRLAGRCEPESLVSL
                     DAGNHALVVCDVEGYEVRLLDPERVPWMRTASLFVELHDCLLAGSSEQVIRRFEPTHD
                     IRKLSSKGHDYSAYPSLEGLTFPEIYAMILEDRPAPQDWMLATPKLS"
     gene            complement(13369..14289)
                     /locus_tag="PLP58_01225"
     CDS             complement(13369..14289)
                     /locus_tag="PLP58_01225"
                     /inference="COORDINATES: protein motif:HMM:NF012888.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfotransferase"
                     /protein_id="HRJ07172.1"
                     /translation="MKTLVYIASTGYSGSTLLESILGSNPRFLNLGEIYKLSYWPVCS
                     CGEKVASCSFWQELERLLQQQSGDASARLQDWHIQGRRGQGKFQRLLHDAFIALGGPE
                     SLGLFKLLSTEVRSFSEGCERALKLFETAAAMTGASVIVDSSKDPLLLKHLYALAPDK
                     LRVIHLVRDARAVSHSFVKNFKRDGTAYVAEDSGAQPTLAQAAEYWKDRNRKIGIASW
                     RIPPDQRLFIRYEDLCLKREDSAAALSRFIGEEIVIPETIHLKQQHTIAGNPMRQTET
                     SIQVRLTEDWQDKLGGEDIAQIAKITGCSW"
     gene            complement(14298..15632)
                     /locus_tag="PLP58_01230"
     CDS             complement(14298..15632)
                     /locus_tag="PLP58_01230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ07173.1"
                     /translation="MSLKTRLRLLLWRVEDDIRYSRRLRAALAWLHWLRGRLARGLEK
                     QARHFSKALSLAETPRSAVFIAEDFRHSLATVAEPVWRRIVASNPRYTPLIQEKPLLT
                     RSVILKAPGPQGEKGALLNYFEYNLARVLALSEVDLAWLDSRYHLIFVASWSPTDYAL
                     LAGALRRLKSPLFIQCANESEREKLAALHPRLICLPGLACDWVDPVFYQPKPNSERSI
                     DILMVANWGAFKRHWEFFAALTRLPAHLRVVLVGQKEGGRDKDFIARLAREIGVPQQL
                     QLLESLPIEKVTELECDARVSVILSRREGGCVAAVESLFAGCALAMRAGAHIGSSAHI
                     NTQTGRRLRPGRIAEDLAALLKDCGTLSPREWACANIRNDLTLARINTVLKSHATALC
                     QPWTTDLATPRWRPHPTLARETDRERLRPAYAELHQRLPGLVSADLMDQSHR"
     gene            complement(15629..16366)
                     /locus_tag="PLP58_01235"
     CDS             complement(15629..16366)
                     /locus_tag="PLP58_01235"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HRJ07174.1"
                     /translation="MNSLGSLLLPKSLHERMQVERWHVHDFIRREALPLMKPGMKVLD
                     AGSGKLPEQYLRGEILDTGATLHTCDLNFGPGVDYEADVSAMPFDDGCYDIVLNTQVL
                     EHVKDPGRVCKELARVLKPGGLLFLTTPQSSPLHNLPWNFFNFTNLGLGLLMDAAGLE
                     RVKVQPQGGHFALLAFELHWTVREIRRSSMPPLLKAPLQLAAQALFGFALKLLLIWLD
                     RFDRDPLNTMGWNLMCRKPDAPATPGA"
     gene            complement(16394..16984)
                     /locus_tag="PLP58_01240"
     CDS             complement(16394..16984)
                     /locus_tag="PLP58_01240"
                     /inference="COORDINATES: protein motif:HMM:NF012360.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine acetyltransferase"
                     /protein_id="HRJ07175.1"
                     /translation="MTFEDLHSFIRADLFRYERAAGWQGFARPFLREPGFRLTCLLRA
                     CRHLRARALPRWTVYPFLKLWLGRISSRLGVYVCHSTDIGPGFYIPHPCGIVINRRCR
                     IGANCNIAQHVTLGLKSREPRMGCPVIGDRVYIGPGAVIIGAITVGSDSAVGANCVVT
                     KDVPAHAVVAGVPGQVISMRGSEGYINDCLPGTADT"
     gene            complement(16981..17886)
                     /locus_tag="PLP58_01245"
     CDS             complement(16981..17886)
                     /locus_tag="PLP58_01245"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HRJ07176.1"
                     /translation="MAVGLWETSLVIHARQSGMRLDDFLMLGRQSLALTPAMLEHLFT
                     CYGTDLRGVDWNAKKWAEPLFEKIGAVQVDSLDYSDYEGARHIHDMNKPWPEGRPPRQ
                     FDVVFDGGTLEHVFNLPQALLNAMSLVKPGGCLLSVSPVDGWLGHGFYQPQPELFFRF
                     LTPERGFRLRGVWLAEFGPAPAKSRLYHIKDPAQSGCRPLVPARRPLAMLVFAEKTSE
                     VSGPPAWPGQSNYTAMWQQATVDQAKGKGAVSLKKALLACVPEVVSRPLKSWLIRRKH
                     ARLARASWEQVARLDTATALDSSAP"
     gene            complement(17890..19035)
                     /locus_tag="PLP58_01250"
     CDS             complement(17890..19035)
                     /locus_tag="PLP58_01250"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HRJ07177.1"
                     /translation="MHPDLLNYLACPKCGGELRVTRGALDNGHVEEGELACQGCPARY
                     AVRNGIPRMIAALNGESARGADDRVDLTVSQYSAYQGEVYAPMAESLQNEAILNARTG
                     LDALDYKDKVCLDAGCGIGRFSRVMARAGARRVIALDAGDSIDAAKKQTDPALPVSFV
                     QGHILELPLKKGCVDRAISIGVLHHTAHPENGFRSIAKAVAEGGTLSIYLYTRAYVPW
                     HKVASVPFWQIRFALWSEPLRRLIVRLPNTPRLAFCKALYVIRMKVLEPLKRAGAPGR
                     FLAKMLQLFLLPDIYKPLENAASNIARNFDAYSTPYNYNHELEELVDWFATEPGFNEL
                     RVTPYRLSITGWKNSAAAPDEPLRVTLHRARSIAALEADGVEHQPAA"
     gene            complement(19057..20913)
                     /gene="asnB"
                     /locus_tag="PLP58_01255"
     CDS             complement(19057..20913)
                     /gene="asnB"
                     /locus_tag="PLP58_01255"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR01536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HRJ07178.1"
                     /translation="MCGICGEYFLNPERQADAARVRAMQHAIEHRGPNDDGQFISGAC
                     GLGFRRLSIIDLEGGHQPIPNEDETVWVTLNGEIYNYPELRRDLEARGHRFRTRSDTE
                     VIVHLYEEHGADFCRHLRGMFGIALHDLRRGRFILARDRLGIKPIYYWQDAEKIVYGS
                     EIKAVLAHPGVSAAPNEAGIFQFLLLRHSLQPDTLFAGIQKLPAASVLVADGGGARVQ
                     EYWRMRHPPALQRDPAQAAAEYEKALANAVDCHLLADVPLGMFLSGGLDSSVLAALVQ
                     QRSNRPVICFTAGFQGGNDESAHAATVARHIGAEHRLIPIQPPPPELLEQLVWHLDEP
                     IGDPACLPTWLLSQEAAKEVRVVLTGEGSDETNAGYSKFLRHHLFHQKAAAMRLARLF
                     WPALRRLPPLSGRFAHYQPLWSAPDELSRILANDQTDPCQPGSLLHDLSPRLAKHHAA
                     TLTRLQAELDACDSRDPTLRLLQYSRSVFMREDLLMKVDRMTMAHGLEARVPYLDHPL
                     AELTAGFAPEVLMRGGQTKAVLRDLSAKLLPPEITRRRQHGFLVPLAGWFQGDFAAYA
                     AGLLSPETIRKRGLLETGQVAGILDRYQKTGEGARMIWNLVLLELWFRRFMD"
     gene            complement(20932..22179)
                     /locus_tag="PLP58_01260"
     CDS             complement(20932..22179)
                     /locus_tag="PLP58_01260"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HRJ07179.1"
                     /translation="MKDTPVHLFLTTELFHPDLPGGLMRFFRYGPGLKERGVTPHVIT
                     LRHRPELRDEDEANGILIHRLDPPDSAPGELQRAWLLRQALRRAREVRLQGGAAVLQP
                     GILSHTMTPSLAGARMRGIGCVHNISIAPETALKTGGIARLRQRLRMGWMCAPVSRFV
                     FLSHQLRRQYQQRWPLRAAQIQVIPNGVDMGRFHPSADAAERAELRARHGLGGGQKIA
                     LFVGGIMARKGVDILLRAWDQVLAEHPDAVLLVLGSRGGRVSHERAGFKDELAEYLRQ
                     MDELRARCAAPESIRFLGEAANPAPYYRMADVFAFPSRREGLPNVVLEAMASALPCLV
                     ARFDGMPQNGEEFGHADRHFHALSHEPGEWAARLARILRDDQDGPRRKMGALAREWIE
                     SHHNLPTILDQWSRLYHEAAGQG"
     gene            complement(22200..23099)
                     /locus_tag="PLP58_01265"
     CDS             complement(22200..23099)
                     /locus_tag="PLP58_01265"
                     /EC_number="2.8.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF012888.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008146 - sulfotransferase activity
                     [Evidence IEA];
                     GO_function: GO:0050656 - 3'-phosphoadenosine
                     5'-phosphosulfate binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfotransferase"
                     /protein_id="HRJ07180.1"
                     /translation="MSPLHLPGGKTAPDLFIIGGARCGSTSLHYYLDQHPGVHMCQPD
                     KEPSYYCDVYGVKDPEEYAANFAGAAPGQLLGDASTPYLTCPASAGLIHAANPGARII
                     VILRQPADRAYSLYLKMCSLGHETAPDFETALELEKGIVNDPPEWRREGYHYNHLYFH
                     SGLYAEQLRRFHELFPKEQIFHVIFDDLTVSPQKLCAEICAWLGLPAMPAINPEPRNR
                     AVRPRSLKFQHWLRHSLNPALKKTRLPWRGQILEKLLAWNTDPSPAPKLDAGLRRRLT
                     ERYAEDIQATAALIGRDLGRWLR"
     gene            complement(23121..24167)
                     /locus_tag="PLP58_01270"
     CDS             complement(23121..24167)
                     /locus_tag="PLP58_01270"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HRJ07181.1"
                     /translation="MIESPPPRPSASLWSSLSLLRFVLAAIVAAAHVKAFYGGTLAAL
                     DPVKILASFNGHAAVVGFLLVSGFSIAHSLLAEGKGYVWRRFVRIYPLYLPAVLFSAW
                     VGGYVVPGGAAGEPITYAAPGLFETLGNLLMLQCVLVPPMSNNGPLWTLSLEWWCYMA
                     APLLAMLSRRRLLLVLVLMTAAHLSWMVLGARFGFYARCPFGLHIVFLGVFWLAGFWY
                     YQVRAAPWAAWSFIGLVWLLTGLNRDNLAGNYQPTLAVSCLMLIAGGLIAWPAWSGGL
                     LKLLGNASYSLYLLHKPFYHLAHENLGLRQGWQIFSLTVAVAILAHWLVELPLRKALQ
                     KLWNHLQGSRSPAT"
     gene            complement(24181..25701)
                     /locus_tag="PLP58_01275"
     CDS             complement(24181..25701)
                     /locus_tag="PLP58_01275"
                     /inference="COORDINATES: protein motif:HMM:NF014051.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HRJ07182.1"
                     /translation="MSLTRKILRGSTLNLVDHAARILAMLVVTPLMVTKLGLEGYGIW
                     LVLTAAVSFLNLLDGGITLSGTRYLARALGGKDAEAAGLVTGTLRWLYRRIGLGCAVA
                     TAGLVLAVPWLVRDAAWQESARLVLLALGGGMTLRFFLRIHLVILKGHLRYDLIVAAS
                     LVKVVLQTALVLALLTRGHGLVVLAVAQIISDLFDQLLVVFFSRRTGGGAHARAGHSP
                     ELLPEILRYSALAFLNTTGQHLRSRIDPFVITSFVGVAAVPVYNMGLRLATLFADMVN
                     AVMGGTLLAGFSQVEGREGVDGVRRKFLFSLRFSIPLALLGAAGLAVLGPAFLLRWVG
                     PDFADSGTVLRLLVPPYALWLMQFPSGSLFLSLNQHHLITKLTFGAGLFNVALSVTLA
                     SWMGFYGVVWATFVDMTLFYGLAVPLLVSRVLGMPLMRYYAVLITPILKLGLALGAAA
                     LLAQPLVKPDYLSLLMAGMALGAAFGAACWWLLMHRDERAVILRKLRPASEVKKAA"
     gene            25906..26697
                     /locus_tag="PLP58_01280"
     CDS             25906..26697
                     /locus_tag="PLP58_01280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ07183.1"
                     /translation="MPAPPSRIKQDQKKWLIAGVLAAAILGGFWSMWLAGPPSTEDDS
                     LVERRKNASPPHLEGSTSGAKDGQTRTTSGEFSSRNWESGFPKAGESSARIQFIHDHV
                     AELDADQARALLVAALRDPEADVRMEALKMSGLLSEREIDKAVLNIALQDSDEHVKDR
                     AFTRVNELPVRERIPFFGNALTGPGEETARKAAEWLGVLGGWDAAAALVAAWPAVAHH
                     PARSAAIQRSLQRLTGQQFNGAGQAADWWAKTSSGLDKDLLPKTP"
     gene            26830..29973
                     /locus_tag="PLP58_01285"
     CDS             26830..29973
                     /locus_tag="PLP58_01285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ07184.1"
                     /translation="MKTSLPGSLSTRIQCAVLAAGLALSTAPSYSQNSIVLTPVAETG
                     ETAPGTVSTWSSLSSSLASVSADGGVSFVGTAGGLGGLWYGEPGSLLLAARQGDAAPG
                     TGANFGTFNIDTTAFAATFEAAFSNTLDDSNDSIWTTTVGPALDLVALEGAATPASLA
                     GKTFSILNQPSVNDNGDIVFRATVEPSPGVRTLWRSINGVGIEHIARNTEPTNIGALP
                     GSTYNVIGDMYCINNDGEVVFRTSLSGIGANNNVLLSMPDPIPGGGPTLVAREGDVAP
                     GAGASTYLDFTACHINNSEDILFRASLGGVAPASANTGAWHYDSATTTITDIAVEGNA
                     TPGFVGGFFDTIEELFLADNGVIVVVAIARDTANDTGSLIGRGVWMDAGAGMELVALV
                     TQNIFDRDGSTVYGTVKNIDQVTMNSDGDIAIELRAGPGASSIKGIWKREAVAAAVAQ
                     RLISVGDTTYDSNRVARVLTGLDLPESEAFTSNGGGPIGRPRTLGDDFIPLLWANYAG
                     GSGLYFADAQSLSFLVTSTNEEAPGTANGTLFDSLRPSGTINENGLIAFRAFLQDTTG
                     DALAGVNLQGIWAELPGGGGVPTLNLAARQGNMTPGGDVYGQMPLNPVFNDNNQIAFS
                     SPVQGGGNALFAGTLASLAEIARSGDAVGAFTGLPTGLVYDLVRSPLAYNNGARLLAS
                     CTFALDEILGVTANNDSAILRHGTSRRMIAREGDAVPAPPAPNPAATGVFGHLYNANV
                     YLNTNHNSAFVAARRVNAALGITSANNAGVFYHNGTTLGTVAIGSTATNIQTAPGTGG
                     AQYLKPAESISFNNANRIAFLTTLQGAGVTLANNLALYSSLNGAAPVLLARTGSTQAS
                     GEGPGGIAAAATFAALGRPHLNSTTTLVFRANLSVGDGGVTVNDDAGIWSNSGAGTTA
                     LLLRKGDFAPDANGNATAAVFDSFDHPVISADGRVVIGATLRVGVGGVTVNDDRAVYA
                     QTSGGVFVLVAREGQALDIADGLGGTVNVNIRDLSYPGSGGAASNSYKAISTSGFVLV
                     NLEFVTGETAMMLFLVP"
     gene            complement(30204..32279)
                     /locus_tag="PLP58_01290"
     CDS             complement(30204..32279)
                     /locus_tag="PLP58_01290"
                     /inference="COORDINATES: protein motif:HMM:NF019335.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HRJ07185.1"
                     /translation="MHPLYFFRHLQFSDFKFRTSLFLAAVCFNGLLCSQEPAAETQTT
                     LPEVVVEGKARPLLGVTDTASKGVATQEDFLSRPLLRRGEILETIPGMVITQHAGGGK
                     ANQYFLRGFNLDHGTDFAVSLDGMPLSFVSHAHGQGYTDLNPVIPELVDSIAYAKGTY
                     TAADGDLSSAGSANFRLVDRLPGHMIKAEFGEYNYYRSLLAGTLPATEAGALTYGLEF
                     NYYDGPWTQPEEFNRWNGLLRWFTGDEDDNLSVTFQGYTGSWTSTDQIPVRASGLGRF
                     DTLDPTSGGDSERYSLNVAMQTRDGDVVTRANVYGIHYSLNLFSNFSYFVNGPLGDQF
                     EQSENRWIFGGQMARTWEKREAFGVEHDFTLGAQTRHDFIDGIGLWNTTRRERTSIIR
                     QDDLMQNSAGVYAELQSRWAPWFRTVLGLRGDLYHFDNQASTARNQEGSEWDGIVSPK
                     FSAIFGPWHDTEYYLSFGTGFHSNDTRGVTASNSPADALVRTLGAEAGLRTQAIEDVT
                     TTLAFFWLRSDSELLYVGDAGTNEPGPGSDRYGVELATYWRPASWFSADAELALTYAR
                     LRGVGGDDRIPNSVPVMFSGGINLGAQGNAPGWFAGARVRAFGARPLDEANEFESRDS
                     LMVNATLGYRRDNWEVALDCLNLLNRADNDIEYAYESQLKSELAPVNGVHAHPVEPRM
                     FRARVTLRF"
     gene            33038..35413
                     /locus_tag="PLP58_01295"
     CDS             33038..35413
                     /locus_tag="PLP58_01295"
                     /inference="COORDINATES: protein motif:HMM:TIGR01494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase
                     metal-binding domain-containing protein"
                     /protein_id="HRJ07186.1"
                     /translation="MKETTACAHCGCPVPQGREDGFCCAGCVYVHDLLHAQGFDHFYD
                     LKGGNALSPVPPQALRERDYAWLEQLAAQTAQAGGETAELRLALQGISCVGCVWLIEK
                     IFARHPGGLRVNVDVVHGEMTLLFQSGVFDAAAFARDMQGFGYLVGPPHEGGERKQSS
                     GLERRMGVCGAFAMNAMAFSLPAYFGMPPDFAFAAWFDMIAAASATLAMLVGGSFFIE
                     KAWRGLQAGALHIDTPIALGIIAAYAGSLGGWLAGVEGLKYFDFVAIFIFLMLAGRWA
                     QQAAVERNRRKLMRDTSIPESVMLPESGRTAPLSALKPGARFAIKPGQSIPVACRLLS
                     PRASVSLEWINGESEAQQREEGQMLPSGALNIGARALEAEALEGWQESTLRRLLEARR
                     PGDFRDLRLERLLRWYLGIVIVIGAAGGLTWWLRGAEAARALQVMISIFVVSCPCALG
                     VAVPLAEELAAARAEKSGVFVRALALWKRLTRARRVVFDKTGTLTLENPVLADALTVS
                     KLSTESRIALRTLVTGNLHPVSRSLFDALGPGRVLDAEVREDIGLGLSLTDDKNRIWS
                     LGRSPAADARLACDGVPLADFCFRDQMRAESIEEVGALRARGLSVHILSGDREEKVAG
                     IAARLGLEKEHWLARMTPEQKARWVAGRDRDDTLYIGDGANDSLAFDAALCAGSPVTG
                     RSFLEQKADFFFLGHSLRFVSALLDIARVHQRATRRVFAFSVSYNIITVIAGLMGHLS
                     PLAAAILMPLSSVATLSLVAFTFRVSKAGRTLPARNAVRRQGGLEGSLAAA"
     gene            complement(35427..36245)
                     /locus_tag="PLP58_01300"
     CDS             complement(35427..36245)
                     /locus_tag="PLP58_01300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxyphosphooctonate aldolase"
                     /protein_id="HRJ07187.1"
                     /translation="MHSRIFFLVVLLAAAFALAGLWMERRGCLDCFARHLMFDSLRLP
                     YPEPPEPEKWSALTAVERLADTRARLLPGLHDELAMRGTSPGAQAYLRAFKESRELEL
                     WLRDGSEWVLFRNYPVAALSGGPGPKQREGDGQVPEGFYAVERGALNPASSFHLSFNI
                     GYPNAHDRHLGRTGSFIMVHGSNVSIGCLAMTDPVIEEIYLIVEAALDAGQRAVPVHV
                     FPFRMTDARMELARESEWHSFWSELRAAHDAFEQTGKPPRVAFEAGRQVITPER"
     gene            36750..37625
                     /locus_tag="PLP58_01305"
     CDS             36750..37625
                     /locus_tag="PLP58_01305"
                     /inference="COORDINATES: protein motif:HMM:NF015585.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialate O-acetylesterase"
                     /protein_id="HRJ07188.1"
                     /translation="MRRVFLLCLLALSSPLFAQKAAKAKKAGTPPPTKPPLVSVDITK
                     LPAGATHLDLWLLCGQSNMKGRGLMPEEPKNDPRIVMMHKMDDQWYLARHPLHLTGDA
                     KTFQGHDNAGVGPGLAFAEVLAAQDKTAAIGLVPCAVGGSSIKLWQKGAKLYEDALRR
                     AKLALQTTAPVKARIRGILWLQGEANANAEELPQHADRLRAMIEAFRTELAQPDLPFI
                     ACTVGEMRDEPQLTHLKAMNDIQLALLKSVPHTACVDARDLKTHIGDNVHFDTAAQEE
                     IGRRFAAEFNKLAGR"
     gene            complement(37626..39365)
                     /locus_tag="PLP58_01310"
     CDS             complement(37626..39365)
                     /locus_tag="PLP58_01310"
                     /EC_number="2.1.1.-"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF023569.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional alpha/beta hydrolase/class I
                     SAM-dependent methyltransferase"
                     /protein_id="HRJ07189.1"
                     /translation="MKSERLQFTADDGESLHYKSWNVQGASRALVLLHRGHEHADRWD
                     SVAPSLLMPDTAIYAWEARGHGQSPGRRGHAAGFMEYARDLDTFFRHLQKQHDLVPEK
                     TVVVAHSVGAVVASAWVHDFAPRIAGLVLATPALEVNLIVPGALTGIRALQKLKPGAT
                     IKSYVRGSWLTRDPAAAAEYDADTSISKDISARILTGLFDTARRVVSDAEVMDRPLLL
                     FSAGADKVVKRGAIDRLYTNYGCDQKIHLTLKGARHAIFHDLCREEVCAAIKRFAVRC
                     IENFTPPRLEADLSHPATNAEFAALKKPLPSPSLKGLSFILQRASLGTLGRLSQGIRI
                     GHATGFDSGESLDYVYENKARGNLLAGKIIDHFYINAIGWRGIRQRRACMNQALRHAL
                     RQAREAGMPPQLMDIAGGAGRYLLDVMADEECGGDLHVLCRDWSESALARGRASASQM
                     GLAERITHARGDAFDEESLASVEPKPSVAVVSGLYELFPDNARLQRSLRGLFRAVPPG
                     GWLIYTGQPWHPQVEMIARTLTNRDGEYWVMRRRPQGEMDALVEQAGFKKEKQWVDDF
                     GIFTVSLARKPVA"
     gene            complement(39534..40613)
                     /locus_tag="PLP58_01315"
     CDS             complement(39534..40613)
                     /locus_tag="PLP58_01315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009958452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter"
                     /protein_id="HRJ07190.1"
                     /translation="MTLVLTVVLVALIFEYINGFHDTANSIATVVSTKVLTPRQAILL
                     ASVTNLVGALFGTAVAKTISSGLVDSQFVSTTTIICALSGGIVWNLITWWFGLPSSSS
                     HALIGGLIGATLASADGNWEALIWSKPPDAGKHWYEGGGIFYKVVLPMVSSPLAGFTF
                     GLVLMGLLNSICYYLRPLVVSRTFGKMQIFSAGYMGFSHGQNDAQKTMGIIALALVAG
                     TNAGDLQELPGWLSFLRIESLGAKGDQIPVWIKVVCALTMAAGTAAGGWRIIKTLGHK
                     MVKLQPVHGFAAETTAATVLFGAASLGMPVSTTHAITTSIMGVGAARRWNAIQWSVVG
                     RILWAWVLTIPATGALGYVFVKAVV"
     gene            complement(40624..41259)
                     /locus_tag="PLP58_01320"
     CDS             complement(40624..41259)
                     /locus_tag="PLP58_01320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007415553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF47 family protein"
                     /protein_id="HRJ07191.1"
                     /translation="MLALQNLFGKNDVFYDLLEASATQARKSVQSLHSLLSAPVGDGA
                     TLEAFAASRREDKKITEQIDEALCRTFVTELEREDISALATALYKIPKTVEKIAERVM
                     IARQHLEGADFSRQITLMDSAADCVVTMITELRSKLHLERVKVLNDKLQKIEGDADKL
                     VLECLRDLYSQTSRGAITIIVMRDLYDLMEKVVDRCRDAGKVVAHIVLKNS"
     gene            41465..44572
                     /locus_tag="PLP58_01325"
     CDS             41465..44572
                     /locus_tag="PLP58_01325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009962108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PSD1 and planctomycete cytochrome C
                     domain-containing protein"
                     /protein_id="HRJ07192.1"
                     /translation="MFSRPTAILFCLSTALHAAPGELRYNRDIRPILSDNCFACHGPD
                     KNHREADLRLDVREAALEMKAIVPGDPAASSIMDRVHTTDEDDLMPPPESKKTLTDAQ
                     KKMLAQWIEQGAPYEPHWAYTPLVKPEFPSFRNPQSAIRNPTDTFIQSRLAEKGHAPS
                     LMAGARTLARRISLDLTGLPPAPQEVERFEKDFAQSPDDAVNALVTRLFKSPHFGERW
                     AVWWLDVARYADTVGFHGDQNQRVFPYRDYVIRSFNVNKRFNQFTLEQLAGDLLPRPT
                     TEQLVATGFNRLNMMTREGGAQPKEYLAKYQADRVRTVGGAWLGATLGCAECHDHKFD
                     PFTMRDFYSLSAYFADIKQFGVYASYGYTPVEELKGWSNDHPFPPEIEVDSPYLKQRL
                     QRTRARMIETAAQAWKDAVKTDSGRASIQSWIEQARAHLQAHPEGWLTPAHEAATRPA
                     KEDKKAPPVQAAVQPDGSVLLETRAPQNTVITLKPGAARIAAIRLELLPHEFHRGIEM
                     GAATNGLTLNPAFTIRQPGADKETPLAILHADAPEKQPRYSGTEEVIGIRGGWKTSVA
                     FARAAQHSVWILDQAVELAEGGALILRLNNHSAGCLRVSISPVAPFLPLEKNWAAALP
                     PLLDKALTSLEKTVHPRIATLWLMQSSQAPASARETWRALYHEALACRDGKAMTQVTV
                     ALAEPMPVRDLPRGNWMDESGALRPPAPPEFLTGPLKPDAPRQTRLDLARWLTSAENP
                     LTARTFVNRLWKQFFGHGLSSAVDDLGAQGETPSHPELLDWLASEFRDSGWDIQHVMR
                     LIVTSHTYLQDSRARPELREADPDNRLLAFQNPRRLDAEFVRDNALFAAGILNLDLGG
                     PSAKPYQPPGYYDNLQFPSRDYIPHTDDRQWRRGLYMHWQRTFLHPMLANFDAPARDE
                     CTAARTVSNTPQQALTLLNDTTFVEAARAFAQSLPAASDEARLGHIYQRALARPPTDK
                     EQRSLLAFLATQRAAFQAAPQDAQKLLATGLRPLPEKAGPAELAAWTSLCRVVLNLHE
                     TLTRY"
     gene            45577..45759
                     /locus_tag="PLP58_01330"
     CDS             45577..45759
                     /locus_tag="PLP58_01330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ07193.1"
                     /translation="MKAHFDFDKHKHELRRIIQDTRAGRMDEEAAVKQLLNPPFRMAG
                     RLEALRMLRVQRAQQG"
     gene            45962..46195
                     /locus_tag="PLP58_01335"
     CDS             45962..46195
                     /locus_tag="PLP58_01335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ07194.1"
                     /translation="MGRIEAYLKGKLMFVAEAEHKIEKAHIIFEDREGFPDLRASERS
                     CLLGVLSFAQKIATEAGVKLELKQTGHIGGMVY"
     gene            complement(46228..46368)
                     /locus_tag="PLP58_01340"
     CDS             complement(46228..46368)
                     /locus_tag="PLP58_01340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ07195.1"
                     /translation="MDILDELPEADEEALALFEEFRASAKEPLPLPDVAYEKFSPFWG
                     EE"
     gene            complement(47191..48105)
                     /locus_tag="PLP58_01345"
     CDS             complement(47191..48105)
                     /locus_tag="PLP58_01345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ07196.1"
                     /translation="MTTQEAEQFCKSSIDRMLKNSQTKVIFPVITLALLQEYTASGKT
                     LFTDGEIRRLYEKTVHELIEFLGHDVHIGGKYYDAYPSRNLPRYQVLKTVSAMTYELL
                     DPYLKFAPELCKWIRERIKAHITMRLGLVPQFHDARTRLEFASSAASFQELLTQHINK
                     TPANFEIFSFAVLKVHLDKFACRLYRDTRTSSHDGGVDLSTNFGVVYQIKKLKLLTEA
                     AATSVYTELKENFDQERLHEGNVILVIDDISRDVKQYLISMKVQSISRDDLLRLAGQF
                     EDVEDRARVLRIVYEEFRREYSSNIKHR"
     gene            complement(48119..48958)
                     /locus_tag="PLP58_01350"
     CDS             complement(48119..48958)
                     /locus_tag="PLP58_01350"
                     /inference="COORDINATES: protein motif:HMM:NF023605.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NotI family restriction endonuclease"
                     /protein_id="HRJ07197.1"
                     /translation="MPQSSQQKHPLAEVFGFPPDANDAAAKRHRSKRLCPFNNKVPNC
                     TKDKAQDPLGVCSVIHGKDLAITCPVRFRQDWLIADHAADFFFPPGTNWTTLTEVRLK
                     DADGCSAGNIDVVLCAYDEQGRVYDFGALEVQAVYISGNVRNPFAQFMSAPETLADMD
                     WTKEPNYPRPDYLSSSRKRLAPQLLFKGGILHAWKKKSAVAIDEAFFRTLPEIPCVPR
                     EESDFALLIYNMLPPTNYHGQYQLTLTRTVYIRSEVSDGHSIEPLSAFIKHLEEAIHL
                     VAV"
     gene            complement(48968..49579)
                     /locus_tag="PLP58_01355"
     CDS             complement(48968..49579)
                     /locus_tag="PLP58_01355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ07198.1"
                     /translation="MPLSWNEIKHRAIKFGNDWKTETREAAERQTFWNEFFDIFGIRR
                     RTVASFEEPVKKLSGNWGAIDVFWPGKLLGEHKSAGQDLDKAHAQGMAYVRGLLDTGR
                     EKEIPRHLIVSDFQRIAIHDLEGGGESLTFPVTDLHKHIRRFAFIAGYKQHRLDPEDP
                     ANIAATDWDAAIRAMLLRITSLRWSRIHPDFFSSRLAILALPA"
     gene            49733..50155
                     /locus_tag="PLP58_01360"
     CDS             49733..50155
                     /locus_tag="PLP58_01360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ07199.1"
                     /translation="MNQEPPQPPPQRCVCDREDTGGCAWMLGLLMLPGVVVLLYQALV
                     LLGLVRDAVMTAEFAVLLLAACGAWCLTLLFSGPERRPLGWLLLGAFVLAGAVFFFIL
                     PLKLPRLPDGMVLLVLALAGLGAFLVVALRVLSGNWGR"
BASE COUNT         9248 a        15464 c        16525 g         9222 t
ORIGIN      
        1 agcggacgcg ccgccgcagc cgccgcgcca tccgggcgcg gctggtaggc cgggttcggg
       61 cggggaaaaa gcgcgccggt ctctttcagg aaggcgtcaa tgcgcgcgtc cagctcccgt
      121 gccttgtccg gcatccgctc cgccatgttc gtcgtctcgc tgatgtcctt ttccagatca
      181 taaagctccc gcggtacgcc gggggcgaac cagcggatga gcttgtgcgc gccgcgcacc
      241 accgtcaccc cgccgccgtt ttgctgggag gggaagaaat tgaaaaactc ctcccgcacc
      301 gccgtcgccg cgccggtcag caccggcacc aggctcaccc cgtccatcac ctggccgggc
      361 ttcttctcca ggccgagcag gtcgagcacc gtggggtaga gatcaatgtg ccccgccagc
      421 gccggcgtca ccgccccctc ccggatgcgc cccggcagcg cccacatcat cggcacgcgc
      481 gtgccgcctt cgtagagctt gcccttgccc tcgcgcagcg ggtggttgtt cgtcggcggc
      541 tcctcccccg cccacttgcg ccagtccgcc aggaacccgc ccttgtgctt ctccgccgcc
      601 gccgtcttgc ccgtgccggg gatgttgctg tgtacattgc cgccgttgtc cgagtaaaaa
      661 atgaccaggg tgttccctgt cagcgccagc gtctccagct cgtcgaggat gcggccgaag
      721 cactcgtcca cactgcgcag catcgaggcc atgatggggt ttcgttgcgc ccctcgcggg
      781 tccgcctttt tggcgaactc cgccgtgaac tccggcttgt gcccccaggg gccatgcacg
      841 ccgtagcacc agagattcag gaaaaacgga cgctccttgt gcgcgcgcag gaacttcacc
      901 gcctccgccg cctggcggtc cacgatgtac tcgcccggcg gcccgtccgt gatggtgccc
      961 accttgctcc gcgccgtggg atggccctcc ggcaccacgc cgtaggggga aaaatactcc
     1021 cccggcggcc ctggatccgg cgcgcaatgc cacgccacat cgaagccctg cgcctccggc
     1081 cggaactccg gcgtcagccc caggtgccac ttgccgatgt gcgccgtggc gtaaccctca
     1141 tcccgcagcg cctccgccag cgtgcgctcg gcggggtcga ggtagttttt gctcaccggg
     1201 ctgatcagcg gccggttcgc cggagccgaa tccggcatga actggaagcc cggcggctgc
     1261 ggcggctggt gcccgctggc ggtcaggatg ccatggcgcg cggggtgctt cccggtcatg
     1321 atgctggcgc gcgtgggcga gcacagcggg ttggcatacg cctgggtgaa gcgcgtggcc
     1381 cgagcggcga agcgatcaat gttcggcgtc tcgtaatatt tggagccata aattccgctg
     1441 tccatccagc ccatgtcatc caccagaaaa aggaccacgt tgggcttgtc agccgccggg
     1501 agggaggcgg cgaggaacag caggagggcg gtgaggatcg ttttcatggc ggcagggcac
     1561 ctcgaaacgc agtcccagtt ccgaacttcc acgaatcagc cagccgggtc aggactcgaa
     1621 gcgccgcccc gatggatagg aacctaggct gcgcaggtgg gcaccgtcgg cgcgggcttg
     1681 gtcgagcgcg gcgcgcacat gcgcctcggc gggggagcct tcgatctcaa tatagaagcg
     1741 gtatttgttc ggctggcccc ggatggggcg ggattcgagg cgcttgagat tgatggcggc
     1801 ctcgctgagc ggggtgagaa agcggcacag gctgcctggg cggtcaggca gttcgacgac
     1861 gagggcggtg cggttgttgg cggggccggg cgcgttcccg gcgcgggcga gaaggtagaa
     1921 ctgggtgatg ttaggcactt cgccggcgat ggggaaatgc aggatgtcca gcgcgtggag
     1981 cgcggcgttt tgccgggggc cgatggcggc cgcgctctgg aggctggcgg ctttttccgc
     2041 cgccttggcg gtgctggcct cgaccacgcg gcgcgcgcgc gggtagttcg cggccagcca
     2101 ttcttcgcag tggaaaaagg gcatggcgtg ggagtgaatg ctggcgactt ccgcgccggc
     2161 gcggccgagg agtgcaaggc gcacatcaag cgtcagttcc tcgatgatgc gcagagtgca
     2221 gcgttcgtct atgaggcggt ccacggtgtc aatgatgaag ccgcctgagg agttctcgat
     2281 gggcacgatg ccctgggctt ccggcgcgtc cgccaggaaa tcaaagacct cgccaatgcc
     2341 ggccatcagg cgcaccggcg tgttcgggaa gcgcatgccc gccagcagat gggagaaact
     2401 gccctcgggg ccgaggcagg cgacgagcgc gggagtgggt gagtctgcgg gcggaggcat
     2461 gggaagtcat ggttccccgc gcctccgccg cggtcaagaa gcgggagaat gcgcgcggca
     2521 ttttcccctc cacatgtgga aggttccgcc aaaaaaaaac agaccccgcc gcgaggcggg
     2581 gtctgcaaga caatgacaag ccgatgaacc cggatcaacg ctttgagaac tggaagcgtt
     2641 tgcgggcgcc ggggcggccg ggcttcttgc gctccttggc gcgtgagtca cgggtgagca
     2701 ggcctgcttt tttcaaggtg ccgcgcagtt cgggattcac ggtgatcaag gcgcgggaga
     2761 tgcccatgcg gacggctccg acctggccgg tgatgccgcc gcccctggcg ttgatgttaa
     2821 agtcatactg ctggcgggtg ttggttaggg ccagcggggt gagaatctgg ttttgcagcg
     2881 ccactgtggg gaagtactcc tcaaaatcac ggccgttgat ggtgatggag ccggagccgt
     2941 ccagaatgtg gacgcggacg acggcagttt tccggcgacc ggtggcggag gtaagcggtt
     3001 tgctcatgaa ggacgggatt cggtgtgtgg gagtatcagg cgagggagaa tggctgcgga
     3061 ttctgggtgg cgtgcgggtg ctccgcgccg gcatagacct tcagcttggt gacgatggcg
     3121 ttgccgaggc ggttgtgcgg caccatgcct ttgacggcgc ggcggacaag ttcctcgggc
     3181 ttgcgggcgc gcagcttttc gacgttctcc accttctggt tgccgatgta gccgccgcgc
     3241 ttgctggtgt aaatcttctg ctgctccttc ttgccggtga gcactacctt gtccgcgttg
     3301 atgacgacga cgaagtcgcc ggtgtccaca tgcggggtga aggtcgggcg ggttttgccg
     3361 cggatgaggt tggcggctgc cacggcgacc tggccgagaa ccttgtcttt ggcgtcaatg
     3421 acccaccatt ggcgtttcac gtcctgggct ttggcggaga atgtcttcat aaagtgcttg
     3481 agggagaggg gcggtggatg gggatgaggc ccattttacc gcgcggaaat ggggcgcaga
     3541 gaatagaagt tcgcgtgcgg gtgtcaaatg gaaatatgac ccgcgcggaa cgccgttcac
     3601 gatgactcaa atccgcgcgg ctgcgagagt cccgcgcgcc gcgtccgccg gaaaaaaact
     3661 ctggcgcggg catgattccg gctctttact caatgccagc ggcgagtaag gccgccctga
     3721 tgataccccc acaggtttat gtcttccctc caggccatcc gcgacgcgct ccagcagtcg
     3781 cctgacaatc cctcgctgct gctgctctat ggccgcgcct gtctggagca gttgcatctg
     3841 gatgaggctc ggggtgcctt cgagcgcgtc ttggagatcg atctggctca cgcggacgcg
     3901 cagatcggga tcgccagagt gcattacctg gaaggggaca cctccggcgc cgcagtgcgc
     3961 gccgagcgtg tgctgcaact cgaacccgga aacgcgcagg cgcatctgct gctcagccgg
     4021 gtttatctga gtgaaaacga ccgcgccagg gcggtggagc actttgatct ggcggcgcag
     4081 gccgacggca gcgtgcatga tcccgccctg gaaaatgaac tcggacgcac cgccagggac
     4141 gcgcgccgct cctcgcccgc accggccatg cccgagcttc ccggcgtgcc ggactccggc
     4201 gaggaaaatg aggcggcgca gggctttttc gacgactcct tcgacgagcc gccctatgac
     4261 tggaggccgg agactttctt cgcgccgggt gatcctcagc gcttcagcac cacgtttgcc
     4321 gatgtcggcg gcatggacga gttgaaggag gagattcgcc tcaagatcat ttacccgctg
     4381 caataccccg acctgtacaa agcgtatggt cgcagcaccg gcggcggcat tctcatctat
     4441 ggtccgcccg gctgcggcaa gacactcctg ctgcgcgccg tggcgggcga ggtgccgtgc
     4501 aattatctct ccgtgggttt gcacgagatc ttcgacccct acttcggcgg cacggagggg
     4561 aacctccacc aaatcttcga gacggcgcgc gcgaacgcgc cctgtgtgct ggtctttgac
     4621 gacgtggact cgctggcgca ggaccgcagg agcgtgcgcg agagccagat gcgcaatctg
     4681 gtgaaccagt ttcttcatga gatggacggc ctgcgcgaca accagcgtgt gctggtcatc
     4741 ggcgccacca accagccctg gcagctcgac cccgccttcc gcaggccggg ccgttttgac
     4801 caggccatct tcgtgcgtcc gccggatgat gccgcgcgcg cccagatcat cgtcctccag
     4861 gcgcgcgaca agccaatctc gaatcttgat gtgcccgcgc ttgtcgaggc caccgcgggt
     4921 ttctccggcg cggacatcga gtgggtcttc ggccgcgccg ccgagatgac gctctccgcc
     4981 gccattcaca gcggccacgc ggtgcccatc ccgatggagt tgctgctcac cgttgcgcgc
     5041 agccacacgc cgagcaccca gaactggttc gacggcatga gggaccacgc cacgcaggcg
     5101 gcgcccgacg ggttcttcaa cgaggtgcgc aagtacctca acgcgccctc caccaaggag
     5161 cgctgatcct ggagcagcgt catgcgggtc gcaggcgaac accaccgcac cgtccgcctc
     5221 gcgggagatg tcgtgcgcgt cattgaccag aggctgctcc cctggcgctt cgagtttctc
     5281 gacctcgcca gcgtggacgc ggcggcccat gccatccgcg aaatggctgt gcgcggcgcg
     5341 ggctgcatcg gcgccacggc gggattcgcc atgaccctgg cagcgcgcga ggcggactcg
     5401 tggcgggagt tggaaaagct ggccggcctt ttgaaagcca cacggcccac tgcggtgaat
     5461 ctggcatggg cggtggaccg ccagattgag gccatccgtg aggcgggctt tgacaggcgc
     5521 gtggagactg ctgtgatgac cgcccaggcc atcgcggacg aggatgcggc ggcgtgcgag
     5581 gccatcggcc ggcatgggct ggacatcatc cgcgccatcg ccgctcgcaa tcccggacgt
     5641 ccggtgaaca tcctcacgca ttgcaacgcg ggctggctgg cctttgtgga caccggctcc
     5701 gccaccgcgc ctgtctatgc cgcccatgcg gcgggcatcc cggtgcatgt gtgggtggat
     5761 gagacgcgcc cgcgcaacca gggcgcgcgg ctcacagcct gggagctggc ccaggagggc
     5821 gtgccgcaca ctgtcatcgc cgacaacacc ggcggccacc tcatgcagca cgcgctggtg
     5881 gacatggtca tcaccggcgc ggaccgtgtg tcgcgcgcgg gcgatgtggc gaacaagatc
     5941 ggcacttatt tgaaggccct ggccgcgcgt gacaatggcg tgccgtttta tgtggcgctg
     6001 ccctcgtcca ccctggactg gagcctgcgt gatggagtgg cggagacacc gatcgagcag
     6061 cgcggggaag gggaggtcac gcacctcgaa ggagtcacca gcgcgggtgt cgtggaaaag
     6121 gtgcgcgtcg tgccggaggg gtcgcccgcc gccaactacg ccttcgatgt caccccggcg
     6181 cggctcgtca ccggcctcat caccgagcgc ggcgtctgcg cggcggagga gggcgccatc
     6241 cgcgcgctgc atggtaaaac ggcgtgagag cgggcgcggc gtgcgcgttt gcaagaggcc
     6301 atgcacatcc gcgccgtcac cggcttcctc tccatcctgc ttctcagcgc cgcgccgcac
     6361 ctgcgcgcgc agtatcccgg caaaacgtgg gagtcattgt cagacgcgga cgccgccgcg
     6421 gcgggctggt cgcgtgagaa gctggcctcc gcgcgcgagt tctccgccac acttcagacc
     6481 gaggcggtca tgattgtcat ccgcggcagg gtgctcgact cctggggcgc ggtggaccgg
     6541 aagttcaaca tccactccat ccgcaagagc ttcctcagcg ccatgtatgg catccaggtc
     6601 gaggcgggaa aaatccggct cgatgccacg atggccagcc tcggcattga cgacaaccag
     6661 ccctccctga ccgaggtgga aaagggcgcc accgtgcgcc agctcctcca ggcgcgctcc
     6721 ggtgtctatc atcccgcgct gtatgagacc gcgagcatga aggcccggcg ccccgcgcgc
     6781 cacagtcact cgcctggcgc cttctggtat tacaacaact gggacttcaa cgcgctcggc
     6841 accatttacg agcagcactg cggcgcgcgc attcacgagg acttctcccg cctcatcgcc
     6901 gctcccatcg gcatggagga ctacatcccc gctgatggca gctatgtgac cggcgccgac
     6961 tccatccacc ccgcctaccc tttccgcatg accgcgcgtg acatggcgcg cttcgggctg
     7021 ctttttctgc gcggcggcaa atggcaggac cggcaggtca ttcccgccgg ctgggtggtg
     7081 gagagcaccg ccagctattc cgatgcgggc gccgccgggg gctacggcta cctttggtgg
     7141 atcgctcaaa gcggcgtgca tcttggcggc gtcaccctgc cggggggcag ctactccgcg
     7201 cgcggcgcgg gcgggcataa aatcctcgtc atccccgcgc ttgatctcgt catcgtgcat
     7261 cgagtcaaca cggacatcga aggccgccag gtctcctccg ccgatttcgg cgcgctcgtg
     7321 cgccgcatcc tcgacgccta tgcgccgccg cccgtctcag gtggtgtgcc ggaggcgctg
     7381 gatgcgctca tgcccgtgct catgagccgc catcatgtgc ccggcgcggc cgtgctcggc
     7441 attgagaatg gccgtgtcgc ctgggaaaag tatctcggcc tgcgcgaggc tggcaagacc
     7501 gcccgcgtgg atgccgccac cgtgttcgag gccgcctcca tgaccaagcc gctggccgca
     7561 taccgcgcgc tccaactcgt cgagcaaggc agcctggacc tcgaccgccc gctggccgct
     7621 tacctgcccg cgccttattt gcaggacgag ccgctgcacg aaaaaatcac cgcccgcatg
     7681 gtgctccagc acagcggcgg ctttcccaac tggcggccca agggcgctgc gctcaaagtc
     7741 atgcacgagc ccggcgccgc ccacctttac tcaggcgagg gctttctttt tttgcagcgt
     7801 gtcattgagc acatcaccgg ccgggactac gagcaggaca tgcaggccgc gctgctgcgg
     7861 cccctgggaa tgaaggacag cagccatgtc tggcaggagc gttttgcctc aagcgccgcc
     7921 gccggccatg acggcaaagg cgcacccaag cccgaccgca gactctacac caagcccaat
     7981 gccgcctaca gcctttacac cacagcgcgc gactatgccg cctttgtcat cgagatgatg
     8041 aaggcggacc gctccgcgcc gcattccctc aaggcggaaa ccctgcgcgc catgctcaca
     8101 cccgccggtc cgcccgcttc gcgcgaatgc ctgacccggc gtggagccaa ggcggagggc
     8161 gtggttcaat acggtctcgg atgggccgtc gaaccctgcg cctccggtcc gcgcatccgc
     8221 cacagcggct ccaacggcac cggcttccgc agccatgtcg agtttgaccc tgtcgccgga
     8281 cacggcctgc tcatattcac caacagcacc tccggcgatg ccttttggcg cgaactttta
     8341 ggcttcatcg ggcggccatg agagcgcgcc atctcaaaat ctgcctttgc ctgttcttca
     8401 ctggctgtgt gccggagaat aaaccggccg ccatgcgttg gagccttttg gagtccgcgc
     8461 acgcggcttc atgggcgcag gcaggcattc ccgatgaagg cgctgttcgt gttcaggatg
     8521 gcgagctagc cttgcagacc ggccagccca tgaccggcgc gcgcttcact gcatgggaga
     8581 gcgccgggct gcccggcgca tattatgcca tcgaatacga ggccatgcgc gtggaggggc
     8641 gggacatctt cggcatggtc acttttcccg tctcaagcca caatgcgcat gccacctttg
     8701 tcctgggcgg ctggggcggc actgtcactg gcatctcatc cattgattac tctgacgccg
     8761 gcgagaacac gacacgcggc gagcagcgct ttgaaaacaa ccgctggtac cgcgtccggg
     8821 tcgaagtgcg tccggatgac ctgcgcgcct gggtggacgg gctcatcgtc gtcaatgcca
     8881 gcatcaaagg ccgcaaggtc ggcctgcgcc ccggctacat tgaccactgc cttccgtttg
     8941 gattcgccac ctggggcacc agcgccagga tacgctcggt ggtcgtcgag aagttgcgcg
     9001 ctcactaagg cgcggacgtg cgagcgcgga gccggacgca tcaagccagc acggccttca
     9061 ccggatgatg cggctcgacg ccggtgaggc gctggtcgag gccctggaac ttgaagctga
     9121 agcgctcgtg ctcgatgccc atgaggtgca ggatggtggc gttgaggtcg ttgatgtgca
     9181 ccgggtcttt gacgatgttg tagctgaagt cgtccgtttc gccgtggacg atgccgcctt
     9241 tgacgccgcc gccggccatc cacatgcaga agttccgcgg atgatggtcg cgtccgtagt
     9301 tgtccttggt gagcgtgccc tgggaataga cggtgcggcc gaattcgccg ccccaaacca
     9361 caagtgtgtc ctggagcagg ccgcgctgct tgaggtccat gacgagagcg gcggtggcct
     9421 ggtcggtgtc tttgatctgg ccgcgcagca gtttgggcag gttgttgtgc tggtcccagc
     9481 cgcggtggag gatctgcacc acgcgggtgc cgcgctcgat gaggcggcgg gccatgatgg
     9541 cgctgtgcgc gtaactgccg gggcggttca catcttcgcc atacatctcc agggtggcct
     9601 tgctctcctt gccgaggtcg gtcagctccg gcacgctgct ctgcatgcgg aaggccatct
     9661 cataattggc gatgcgggtc tggatgtcgg ggtcgccgat gcgggagtgg ttgatgtggt
     9721 tgaggcggtt gagcgcgtcg agcatggcgc ggcggtcgcc ctgctgcacg ccgggcgggt
     9781 ttttgacaaa gagcacgggg tcaccctgcc cgcgcagggc cacgccagtg tattgcgtgg
     9841 gcaggaagcc gctgctccac atgcgggtga agagcgcctg cgcctggctt tccttcggga
     9901 agctgggggt gaagacgaca aaggcgggca ggtcattgct ctcgctgccc aggccgtagg
     9961 cgagccagga gccgaggctg gctttccctg gcacctcgct gccggtcatg acgaaggtgc
    10021 aggccgggtc gtgattgatg gcgctggtgt ggacggactt catgagcgcg atctcgtcca
    10081 cgatcttcgc cgtgtgcggc atgagctcgc tgacccacat gccgctgtgc ccatgctgtt
    10141 ggaactggaa catggagggt gccaccggca ggcgcgcctg gccgctggtc atggtggtga
    10201 tgcgctggcc cttgcggatg ctttcaggca ggtccttgtc gtagtgctcg cggagctgcg
    10261 gcttgtaatc ccaaaggtcg agctgcgagg gcgcgccgtt catgtggaga tagatgaggc
    10321 gcttcgcctt cggggcgaaa tgcggcagcc ctggcagcgg cggatgcacc gcgccgacgg
    10381 cgggcggggc ggcgctggcg agatcacggc ccatcatgga ggccagggcg atgccgcccg
    10441 cgcgcaggcc catgccctcg aaaaagcggc ggcgtgtctg caggaggtga tgctggaaaa
    10501 aggggtccat ggcacctgca aaaacgaagc tggaaaggga cggctatcca gtggatgaag
    10561 ggcaagacaa aaagaagttg ccgagtatgg catgtatgcc atactcgcga catgaaaatg
    10621 accatgcaca tcaatgagga cgtcttggcc gacgtcatgg agatcaccgg cgccagcagc
    10681 aagaccgccg ccgtggagag agccttgacc gagatggtgc ggaagcaccg gttgaaatgc
    10741 gtcttgcggg agggcatggg catgactccc gaggaaatcc gggagtccta tgacttcagt
    10801 cacgctgacc tgcccgagac gcctctgagc ttcgagcgca aacccgtcaa atactccgcc
    10861 aaaaatgcag ggaagtcttc tcgctgacag cagcttttac atcgggcggc tgcgcgaggg
    10921 caaggacccg ctggccgagc tggccgccat ctccgaagaa tgggaggtgg tgacctgcgg
    10981 cgtggtggtg gtggaagtgt gccggggttt taaaaatgag cggatgcgcg agcgcttcga
    11041 gcgcgccttt tccaccatga tcatgatccc caccacgccg aagatctggc acagagccat
    11101 ggagctggca tggaagctgg atcgtgaggg caagggcatg caggtcaccg atctcgtcat
    11161 cgccacctgc gcgctgcaag tggaggccac cgtgctcacg ctcgacagcg atttcctgcg
    11221 cgttccaagc cttgatgtcg tcgccgagcc gcccttgtaa cgccggctga acgatggcag
    11281 gggagcgggt cgttttttcc cgctcatgag aaccgccttc gccgccgcaa cctgcctcgt
    11341 cctcctcgcc tcctgccagc ctgccgacga gagcgacggc tttgtgccgc ttttcaacgc
    11401 gaacgacctg caaggttggg tgaacgccaa ctgcgcgcct gagacatgga ctatcaagaa
    11461 cggcatcatc tcctgtaccg gcaagcccgt cggagcgctg cgcacggagc ggcagtatga
    11521 gaacttcatt ctggaaatcg agtggcggca cctcagcgag ggcgggaact cgggcgtctt
    11581 tgtctggggc ggcccggtca acgcgcccgg cgtgcccttt ctgcgcggca tcgaggtgca
    11641 ggtgctcgat cacggctaca tgaagaaggc ggacccgaaa aagccgaagt ggtttaccac
    11701 gcacggcgac gttttcccca tccacggcgc caccatggag ccgcatggcg agcacaatgg
    11761 catgcgcagc ttccccagtg aggagcgctc catgcccagc ccggagtgga atcattaccg
    11821 catcgaggcg aacaacggcc gcatcaccct cgccgtgaac ggcaaggtgg tcagcggcgg
    11881 tgacaactgc aatccgagca aaggctatct ggccctggaa tccgaaggcg cgccggtgga
    11941 gttccgcaac gcgcgcatca aggagctgcc ctccagcaac cctccagcgg agatgatctc
    12001 ccctctcgat gaaggctgga agtgcctcta caccggcacc gacttccgcg gttggaaagt
    12061 gcccgccgca ggcggcgaaa aatgggaaag cgccgactgg cagatcaagc tcaagcccgg
    12121 ccagacgggc tccgcgctct ggaccgagca ggaatatggc gactgcgagg tcatctgtga
    12181 tgtgcaactg ccgaaggaca ccgatctcag caaacccgcc gccggcctct gcctgcgcgg
    12241 acacagccac ccggtcgtca tgctcggcca gggggaggcg cccgtagtgc tcggggcgga
    12301 tcaaatcagc cccggcaaat ggtatcgcat caaagccagc ctccagggcg acaagctgaa
    12361 ggtgctcgtc accgaaaccc aggaggccaa cccgcgcagc ttcgaagcca ccgtggaccg
    12421 caacccgcgc ggcagcatcg gcctggccga cctcgcgcag ccggtcgtct atgggaatgt
    12481 gaatgtgcgg gagctgtgat tggaatcagg cgggacttgt gcttgcgcgc ggagggacct
    12541 tcctgcaagg atgacgcatt cctcccatgc gccgcgcatt cagtttgatc tgccagacag
    12601 ccctctcgat cccgctcttt aacaaggtca tgaccaccgt ggcctacgag cagaaacggc
    12661 tgctcagacg aagagtggag gagcgtctgc gcgcggcggg cctttaccct gatgtcgtga
    12721 tgcaggggcc gctgaaaggc acgcgctttc cgccgggtta tgcctcctgc agattcgaga
    12781 aaatcatcgg gtcctacgag gcggagacgc acgcctggct ggatgagctg atcgccaggg
    12841 gcgggttcac ctccatcgtg aacatcggcg ccgcagaggg cagttatgca agcgggcttg
    12901 ccagacattt cccggagatt cccatctttg cgtttgaagc caatgaggcc ctgcaccccg
    12961 ccatccatga gatggcggcc ctgaatgacg tggagggaag actgcggctg gcgggcaggt
    13021 gcgagcctga gtccctggtc tctttggatg ccggaaacca tgccctggtg gtctgcgatg
    13081 tggaaggcta tgaagtccgg ctgcttgatc ctgagcgcgt gccctggatg cggacagcca
    13141 gtctttttgt cgagctgcat gactgcctgc tggccggctc ctcggagcag gtgatccggc
    13201 gcttcgaacc tacacatgac atccgcaagc tctccagcaa aggccacgac tactccgcct
    13261 acccttccct ggaaggactc acctttccgg agatttacgc gatgattctg gaggaccggc
    13321 ccgcgccgca ggattggatg ctggcgacgc cgaagctatc ctgagaggtc accacgagca
    13381 gccggtgatt ttggcgattt gagcgatgtc ttcgccgccg agcttgtcct gccagtcttc
    13441 cgtgagcctg acctggatgc tggtctccgt ctggcgcatc gggtttccgg cgatggtgtg
    13501 ctgctgcttg aggtggatgg tttcagggat gacgatttcc tcgccgatga agcggcttag
    13561 agctgccgcg gagtcctcgc gtttgaggca gagatcctca tagcgaatga aaagccgctg
    13621 atcgggagga atgcgccagg aagcgatccc aatctttcgg ttccggtcct tccaatactc
    13681 cgccgcctga gccagggtgg gctgcgcgcc gctgtcttcg gcgacgtagg cggtgccgtc
    13741 gcgcttgaaa tttttcacga acgaatgact caccgcgcgg gcgtcgcgca caagatggat
    13801 gacgcgcagc ttgtccggcg ccagcgcgta gaggtgcttg agcagcagag gatccttcga
    13861 ggagtccaca atgacggacg cgccggtcat tgcggcggcg gtttcgaaga gtttcaacgc
    13921 gcgttcacaa ccctcgctga aggagcgcac ctccgtggaa agcagtttaa acaagcccag
    13981 ggattccggt ccgcccaggg cgatgaaggc atcatgaagg aggcgttgaa atttgccctg
    14041 gccgcgccgg ccttggatgt gccagtcctg gagacgagcg gaggcatcgc cggactgctg
    14101 ctggagaagg cgctccagtt cctgccagaa ggagcatgac gccactttct caccgcagga
    14161 gcacacgggc cagtaactga gtttatagat ctctcccagg ttcaaaaagc gcgggttgct
    14221 gccgagaatg ctctccagga gtgtggagcc ggaatagcct gtgctggcga tatagacgag
    14281 ggtcttcatg cgggagatca gcggtggctt tgatccatca aatcggcgct gacaaggccg
    14341 ggcagccgct ggtgcagctc cgcgtaggcg gggcgcaggc gttcgcgatc agtttcgcgg
    14401 gccagggtag ggtgcgggcg ccagcgcggg gtggcgagat cggttgtcca gggctggcag
    14461 agggctgtcg catgactttt gagcaccgtg ttgatgcggg cgagggtgag gtcgttgcga
    14521 atattggcac atgcccattc acgagggctg agtgtgccgc agtctttgag caaggcggcc
    14581 aggtcttcgg cgatgcggcc cggacgcagg cggcggccgg tttgcgtgtt gatgtgcgcg
    14641 gaggagccga tgtgcgcgcc ggcgcgcatg gccagggcgc agccggcgaa gagggactcc
    14701 acggcggcca cgcagccgcc ttcgcgacga ctgaggatga ctgacactcg cgcgtcgcat
    14761 tcgagttcgg tgactttttc gatgggcagg gattcgagca gttggagctg ctgcggcacg
    14821 ccgatctcgc gcgcgagacg ggcgatgaag tccttgtccc tgccgccttc cttttgtccg
    14881 acaagcacga ctcgcagatg cgcgggcagg cgggtgagcg cggcgaagaa ttcccagtgc
    14941 cgcttgaagg cgccccagtt ggcgaccatg aggatgtcaa tggagcgctc gctgttcggt
    15001 ttcggctggt aaaagaccgg gtccacccag tcgcaggcca ggccgggcag gcagatgagg
    15061 cgcgggtgca gggcggcgag tttttcgcgc tcggactcgt tggcgcattg gatgaaaagc
    15121 ggcgacttga ggcgccgcag cgcgcctgcg agcagggcgt agtcggtggg tgaccagctc
    15181 gccacaaaga tgagatggta acgcgagtcg agccaggcca ggtccacttc agagagtgcg
    15241 agcacgcgcg cgaggttgta ctcgaagtag ttgagcagcg cgcctttttc tccctgggga
    15301 ccgggcgctt tgaggatgac gctgcgggtg aggagcggct tttcctggat gagcggcgtg
    15361 tagcgcggat tggaggcgac gatgcgacgc cagaccggct cagccacggt ggcgagggag
    15421 tggcggaaat cctccgcgat gaagacggcg gagcggggtg tctcggcgag gctgagcgcc
    15481 ttggagaaat gccgcgcctg cttttcgagg ccgcgcgcca gccgtccacg cagccagtgc
    15541 agccatgcca gggcggcacg caggcggcgc gagtaacgga tatcgtcctc cacgcgccag
    15601 agcaggagtc gcaggcgggt cttcaggctc atgctccggg cgtggcgggg gcgtcaggtt
    15661 tgcggcacat gaggttccat cccatcgtgt tgagcgggtc gcggtcaaag cggtccagcc
    15721 agatgagcag cagcttgaga gcgaagccga agagcgcctg cgcggcgagc tggagcggcg
    15781 ccttgagcag gggcggcatg gaggagcgcc ggatttcgcg cacggtccag tgcagttcga
    15841 aggccagcag ggcgaagtgt ccgccctgcg gctgcacttt cacgcgctca agtcccgcgg
    15901 cgtccatgag caggccaagg ccgaggttgg tgaagttgaa gaaattccac ggcaggttgt
    15961 gcagcggcga ggattgcgga gtggtgagaa aaagcaggcc gccgggcttg agcacgcggg
    16021 ccagctcctt gcataccctg ccggggtctt tcacatgctc gagcacctgg gtgttgagca
    16081 cgatgtcgta gcagccgtca tcgaagggca tggcggagac atccgcttcg tagtccactc
    16141 cggggccgaa gttcaggtcg caggtgtgga gggtggcgcc ggtgtcgagg atttccccgc
    16201 gcaggtattg ctcgggcagc ttgccggagc cggcgtcgag cactttcatg cccggcttca
    16261 tgagcggcag agcctcccgg cggatgaagt catgcacatg ccagcgctcc acctgcatgc
    16321 gctcgtggag ggacttgggg agcaggagtg agccgaggga gttcatgata taaaaaacgg
    16381 atgctttgaa gtgtcaggtg tccgccgttc cgggcaggca gtcgttgatg tagccctcgg
    16441 agccgcgcat ggaaatgacc tggcctggca cgccggccac aacggcatgt gcgggcacgt
    16501 ctttggtgac gacgcagttg gctccgacgg cggagtcgga gcccaccgtg atggcgccaa
    16561 tgatgacggc tccagggccg atgtaaacgc ggtcgccaat gaccggacag cccatgcgcg
    16621 gctcgcggct tttgaggccg agtgtgacgt gctgggcgat gttgcagttc gctccgatcc
    16681 ggcagcgccg gttgatgacg atgccgcagg gatgagggat gtaaaagccg gggccgatgt
    16741 cggtgctgtg gcagacataa acgcccagcc gtgacgagat gcggccgagc cagagcttga
    16801 gaaaaggata aaccgtccag cgcggcaggg cgcgcgcgcg gaggtggcgg caggcgcgca
    16861 gcaggcaggt gaggcggaag cccggctcgc gcaggaaagg ccgcgcgaag ccctgccatc
    16921 ccgccgctct ttcataacga aagaggtcgg cgcgaatgaa gctgtgcaaa tcctcgaagg
    16981 tcatggcgcg gatgaatcca aggccgtggc ggtgtccagc cgtgccacct gctcccaact
    17041 ggcgcgggcc aggcgggcgt gtttgcgccg gatgagccag cttttgaggg ggcgtgaaac
    17101 gacttcgggg acgcaggcca gcagggcttt tttcaaagag acggcgcctt tgcctttggc
    17161 ctggtccacg gtggcctgct gccacatggc ggtgtagttg ctctggccgg gccaggcggg
    17221 cgggcctgaa acttccgagg tcttttcagc aaagacgagc atggccagcg ggcggcgcgc
    17281 cggcaccagc gggcggcagc cgctttgcgc ggggtctttg atgtgataaa ggcgggattt
    17341 ggcgggagcc ggtccaaact ccgccagcca cacgccgcgc agacggaacc cgcgctccgg
    17401 ggtcaaaaag cggaagaaca gctcgggctg cggctggtaa aagccgtggc cgagccatcc
    17461 gtccaccggt gacacgctca acaggcagcc gcccggtttg acgaggctca tggcgttgag
    17521 cagggcttgg ggcaggttga agacatgctc cagggtgcct ccatcaaaga ccacgtcgaa
    17581 ctggcgcggc ggacgcccct ccggccaggg cttgttcatg tcgtggatgt gcctggcgcc
    17641 ttcgtagtcg gagtagtcga gggagtccac ttggacggcg ccgatttttt caaagagcgg
    17701 ctcggcccat tttttggcgt tccagtccac gccgcgcagg tcagtgccgt agcaggtgaa
    17761 aaggtgctcc agcatggcgg gagtgagcgc cagggactgc ctgccgagca tgagaaagtc
    17821 gtcgagcctc atgccggact ggcgggcatg aatgacaaga gaggtttccc aaagtccgac
    17881 ggccatgatt cacgcggcgg gttgatgttc gactccgtca gcctccaggg cggcgatgga
    17941 ccgcgcgcgg tgcagggtga cgcgcagcgg ctcgtccggc gcggcggctg agtttttcca
    18001 gccggtgatg gacagccggt agggcgtgac gcgcagctcg ttgaagccgg gttcggtggc
    18061 gaaccagtcc accagctcct ccagctcgtg gttgtaattg tagggcgtgg agtaggcgtc
    18121 gaagttgcgc gcgatgtttg acgcggcgtt ttcgagcggc ttgtagatgt caggcagcaa
    18181 aaagagctgg agcattttcg cgagaaaacg acccggtgct cccgcgcgct tgagcggctc
    18241 cagcactttc atgcggatga cgtagagcgc cttgcaaaaa gcgagccgtg gtgtgtttgg
    18301 caggcgcacg atgaggcggc gcagcggctc cgaccacaag gcgaagcgga tttgccagaa
    18361 gggcacgctg gcgactttgt gccagggcac ataagcgcgc gtgtagaggt agatggaaag
    18421 cgtgccgcct tccgccaccg ccttggcgat ggagcggaag ccattctcgg gatgcgcggt
    18481 gtggtgcagg acgccgatgc tgatggcgcg gtcaacgcag ccttttttca gcggcagttc
    18541 caggatgtga ccctggacaa aggagacggg cagggcgggg tcggtctgct ttttggccgc
    18601 gtcaatgctg tcgccggcat ccagggcgat gacgcgcctg gctcccgcac gcgccatgac
    18661 gcggctgaat cgtccgatgc cgcagcctgc gtccaggcag actttgtctt tgtaatccaa
    18721 tgcatccagc ccggtgcggg cattgaggat ggcctcgttt tgcagggact cggccatcgg
    18781 ggcatagacc tcgccctggt aagcggagta ttgggacacg gtgaggtcca cgcggtcgtc
    18841 cgcaccgcgc gcggactcgc cattgagcgc ggcgatcatg cgcgggatgc cgttgcgcac
    18901 ggcgtagcgg gcggggcagc cctggcaggc cagctcccct tcctcgacat ggccgttgtc
    18961 caatgccccg cgtgtgacgc ggagttcgcc gccgcacttg gggcaggcga ggtagttgag
    19021 caggtcggga tgcatggagc agtgtcggga gaggggtcag tccataaagc gccggaacca
    19081 gagttccagc aaaacaaggt tccaaatcat gcgggcgcct tcgccggttt tttggtagcg
    19141 gtcgaggatg cccgccactt gtccggtctc cagcaggccg cgcttgcgga tggtttcggg
    19201 ggaaagcagg cctgcggcgt aggcggcgaa gtcgccctgg aaccagcctg ccagcggcac
    19261 gaggaagccg tgctggcggc ggcgggtgat ctccggcggc aggagcttgg cggagaggtc
    19321 gcgcaggacg gccttggtct gcccgccgcg catgaggacc tcgggcgcga aaccggcggt
    19381 gagctcggcc agcgggtggt cgaggtaggg cacgcgcgcc tccaggccgt gggccatggt
    19441 catgcggtcc actttcatga gcaggtcctc gcgcatgaag acggagcggc tgtattgcag
    19501 caggcggaga gtgggatcgc ggctgtcgca ggcgtccagc tcggcctgaa ggcgcgtcag
    19561 ggtggcggcg tggtgctttg ccaggcgcgg gctgagatcg tgcaggaggg agccgggctg
    19621 gcaagggtct gtctggtcgt tggcgaggat gcggctcagc tcgtctggag cgctccaaag
    19681 cggctggtaa tgggcgaatc gtccgctcag cggcggcagg cggcgcagag cgggccagaa
    19741 aagccgcgcc aggcgcatgg cggcggcttt ttgatggaag agatgatggc ggaggaactt
    19801 gctgtagcct gcgttcgtct cgtcggagcc ctcaccggtg agcacgacgc gcacttcttt
    19861 ggcggcttcc tgtgacagca gccaggtggg caggcaggcg gggtcgccga tgggttcgtc
    19921 aagatgccag acgagctgct caagcagttc gggggggggc ggctggatgg ggatgaggcg
    19981 gtgctcggcg ccgatgtggc gggcgacggt ggcggcatgg gcgctttcgt cattgccgcc
    20041 ctggaacccg gcggtgaagc agatgacggg acggtttgag cgctgctgga cgagcgcggc
    20101 cagcacgctg gagtcgaggc cgccgctcag gaacatgccc aggggcacat ccgccagcag
    20161 gtggcagtcc acggcgttgg cgagcgcttt ttcgtattca gcggccgcct gcgcggggtc
    20221 gcgctgcaag gccggcggat ggcgcatccg ccagtactcc tggacgcgcg cgcccccgcc
    20281 gtcggccacg aggactgagg cggcggggag cttttgaatg cctgcgaaca gggtgtccgg
    20341 ctgcaaggag tggcgcagca gcaggaactg gaaaatgccc gcctcattgg gcgcggcgga
    20401 gacgccggga tgcgccagca cggccttgat ctcggagcca tagacgatct tctccgcgtc
    20461 ctgccaatag tagatgggtt tgatgcccag gcggtcgcgc gccaggatga atctgccccg
    20521 gcgcagatca tgcaaggcga tgccgaacat gccgcgcaga tgcctgcaaa agtccgcgcc
    20581 gtgctcctcg taaaggtgga cgatgacctc ggtgtccgag cgtgtgcgga agcggtgccc
    20641 gcgcgcctcc aagtcgcggc gcagctcggg gtagttgtag atctcaccat tgagggtaac
    20701 ccagacagtt tcatcctcgt tggggatggg ttggtgcccg ccctccaggt ctatgatgct
    20761 cagcctgcgg aaacccaggc cgcaagcgcc gctgatgaac tggccgtcgt cattgggacc
    20821 gcgatgctcg atggcgtgct gcatggcccg cacgcgggcc gcgtccgcct ggcgctcggg
    20881 gttgagaaag tattcgccgc aaatgccgca catgtttgat aaaggatgag atcagccctg
    20941 gccggcggct tcatgataca gccgggacca ttgatcgagg atggtgggga ggttgtggtg
    21001 ggattcaatc cattcgcgcg ccagggcgcc cattttacgg cgcggtccgt cttggtcgtc
    21061 gcgcaggatg cgggccaggc gtgccgccca ttcccccggc tcgtggctga gcgcgtgaaa
    21121 atgcctgtcc gcgtgtccga actcctcgcc attctgtggc attccgtcaa accgcgccac
    21181 caggcatggc agggcggatg ccatggcctc cagcacgaca ttcggcaggc cctctctgcg
    21241 tgagggaaag gcaaagacat ccgccatgcg gtagtaaggc gcggggttcg cggcctcgcc
    21301 gagaaagcgg atgctctccg gcgcggcgca gcgggcgcgc agttcgtcca tctggcgcag
    21361 atactccgcc agttcgtctt tgaagccggc gcgctcatgc gagacgcggc cgccccggct
    21421 gccaaggacg aggagcaccg cgtcgggatg ttccgccagc acctggtccc aggcgcgcag
    21481 caggatgtcc actcccttgc gcgccatgat gccgccgaca aacagcgcga ttttttggcc
    21541 gccgccgagg ccgtgccggg cgcgcagttc cgcgcgctcc gccgcgtccg cgctgggatg
    21601 aaaacggccc atgtccacgc cgttcgggat cacctggatc tgcgcggcgc gcaggggcca
    21661 gcgctgctgg tattgccggc gcagttgatg gctgagaaaa acaaaacgcg agaccggggc
    21721 gcacatccag cccatgcgca gcctttgacg caagcgcgcg atgccgccgg tcttcaaggc
    21781 ggtttccggg gcgatgctga tgttgtggac gcagccgatg cccctcatgc gcgcgcctgc
    21841 cagggacgga gtcatcgtgt gggaaaggat gccgggctgc aagacagccg cgcctccctg
    21901 aaggcggacc tcccgagccc gacgcaacgc ctgccgcagc agccacgcgc gctgaagctc
    21961 ccccggagcc gagtccggcg ggtccaggcg gtggatgagg atgccgttgg cctcgtcctc
    22021 atcccgaagt tcgggacgat gacgcagggt gatgacgtga ggcgtgacgc cccgctcctt
    22081 gaggccgggt ccgtagcgga agaaacgcat cagcccgccc ggcaggtcgg gatggaaaag
    22141 ctccgtggtc aggaaaagat ggacgggcgt gtccttcaca tcaaggcgtg gtgaatgagt
    22201 cagcgaagcc agcgcccgag gtcgcgtccg atgagggcgg cggtggcctg gatgtcctcc
    22261 gcgtagcgct ccgtcaggcg gcggcgcagt ccggcgtcga gcttgggcgc agggcttgga
    22321 tcggtgttcc aggccagcag tttttcaaga atctgtccgc gccagggcag tcgcgttttc
    22381 ttcaacgcgg gattcaggct gtgcctcagc cagtgctgga atttcaggga gcgcggacgc
    22441 accgcgcggt tgcgcggctc gggattgatg gcgggcatgg cgggcaggcc gagccaggcg
    22501 cagatttccg cgcagagctt ttgcggggag acggtcaggt catcgaagat cacatgaaag
    22561 atttgctcct tgggaaagag ctcgtggaag cgccgcaact gctccgcgta gaggccggag
    22621 tggaagtaga ggtgattgta atggtagccc tcccgccgcc actccggcgg gtcgttgacg
    22681 atgcccttct ccagttccag cgcggtttca aagtccgggg cggtttcatg cccaaggctg
    22741 cacattttca gatacaggga atacgcgcgg tccgctggct ggcgcaggat gacaatgatg
    22801 cgcgcgccag ggttcgccgc atggatgagg ccggcgctgg cggggcaggt gaggtagggt
    22861 gtggaggcgt cgccgagaag ctggccgggc gcggccccgg cgaaattggc ggcgtattcc
    22921 tccgggtctt tgacgccata gacatcgcag taataactgg gttccttgtc cggctggcac
    22981 atgtggacgc cggggtgctg gtccaggtag taatgcaggg aggtgctgcc gcagcgcgcg
    23041 ccgccgatga tgaaaaggtc gggggcggtt ttgccaccgg gcaggtggag gggggacaag
    23101 ggagccaaaa cagggggagg ttacgttgcg ggagagcggc tgccttgtag gtggttccag
    23161 agtttttgca aggctttgcg cagcggcagt tcgacgagcc agtgcgcgag aatcgccacc
    23221 gctacagtga ggctgaaaat ctgccagccc tgcctcagtc ccaggttttc atgggccaga
    23281 tggtagaaag gcttgtgcag caaatatagg gaataggagg cgttgccgag caacttgagc
    23341 aggccgccgg accatgccgg ccaggcgatg agtccgcccg cgatgagcat caggcatgac
    23401 acggcaaggg tgggctggta gttgcccgcg agattgtcgc ggttcaaacc cgtaagcagc
    23461 cagaccagcc cgatgaagga ccatgccgcc cagggcgcgg cgcggacttg ataataccag
    23521 aatcccgcca gccaaaagac gccgagaaac acaatgtgca gcccgaacgg gcagcgggcg
    23581 taaaacccga agcgcgcgcc caggaccatc cagctcaggt gcgccgccgt catcaagacc
    23641 agaaccagca aaagccgccg ccgggaaagc atggccagga gcggcgcggc catgtagcac
    23701 caccactcca ggctcaaggt ccacagcggg ccgttgttcg acatgggcgg aaccagcacg
    23761 cattgcagca tgagcaggtt gcccagcgtc tcgaagagcc ccggcgcggc gtaggtgatg
    23821 ggttcgccgg ccgcgccccc gggcacgaca tagccgccca cccaggcgga aaacaaaacc
    23881 gccggcagat agagcgggta aattctcaca aacctgcgcc agacataccc tttgccctcc
    23941 gccagcagcg agtgggcgat ggagaatcct gaaacgagaa ggaatcccac cacggccgca
    24001 tgaccgttga acgaggcgag gattttcacc gggtccagcg cggcaagcgt gccgccatag
    24061 aaggctttca catgcgccgc ggcgacgatg gcggccagca cgaaacgcag cagagacaac
    24121 gaggaccaaa gcgaggcgga ggggcgggga ggcggggatt caatcatggc agggagtcat
    24181 tcacgcggct tttttcactt cggatgcggg gcgcagtttc cgtagaatga ccgctctttc
    24241 atcccggtgc atgagcagcc accagcaggc cgcgccaaaa gccgcgccaa gcgccatgcc
    24301 cgccatgagc agggagagat aatccggttt gaccaagggc tgtgctagca aggctgcggc
    24361 gcccagcgcc aagccgagct ttagtatcgg cgtgatcagc accgcataat agcgcatgag
    24421 cggcattccc aaaacccggc tcacgagcag cggaaccgcc aggccgtaaa aaagtgtcat
    24481 gtccacgaag gtggcccaga ccacgccgta aaagcccatc caggaggcca gcgttacgct
    24541 aagagcgaca ttaaacaaac cggcgccaaa ggtgagcttg gtaatgagat gatgctggtt
    24601 caggctcagg aaaagactgc cggaagggaa ctgcatgagc cagagcgcgt agggcggcac
    24661 cagcagccgc agcacggtgc cggagtccgc aaagtccggc cccacccagc gcagcagaaa
    24721 ggccgggcca agcacggcca gccccgccgc gccgagcagc gccagaggga tgctgaaacg
    24781 caggctgaac aggaacttgc gacgcacacc atccacgcct tcacgcccct ccacctgact
    24841 gaagcctgcc agcaatgtgc cgcccatgac cgcgttgacc atgtccgcga aaagggtggc
    24901 caggcgcagc cccatgttat agaccggcac ggcggccacg ccgacaaagc tggtgatgac
    24961 aaaggggtct atgcggctgc gcaggtgctg gccggtggtg ttgagaaagg ccagggcgga
    25021 atagcgcagg atctccggca gcaattcggg actgtgcccc gccctggcgt gcgcgccgcc
    25081 gccggtgcgg cgcgagaaga agaccaccag tagctggtcg aacaaatcag agatgatttg
    25141 cgccaccgcc agcaccacca ggccgtgccc gcgcgtcagg agcgccagca cgagggcggt
    25201 ttgcagcacc accttgacca gggaggctgc cacgatcagg tcatagcgca gatggccttt
    25261 gaggatgacg agatggatgc gcaggaaaaa acgcagcgtc attccgccgc ccagcgccag
    25321 cagcacgagg cgggcgcttt cctgccaggc tgcgtcccgc accagccagg gcacggccag
    25381 gaccagtccc gccgtcgcca ccgcgcagcc gaggccgatg cggcggtaaa gccagcgcaa
    25441 tgtgccggtg acgaggcccg ccgcttcagc gtctttcccg cccagcgcgc gcgccagata
    25501 gcgcgtgccg gagagggtga tgcctccgtc cagcagattg agaaaggaca ccgcagccgt
    25561 cagcaccagc cagatgccgt agccctccag ccccagcttg gtgaccatga gcggcgtgac
    25621 caccagcatg gccagaatgc gcgccgcgtg atccaccaga ttgagcgtgg agcctcggag
    25681 aatctttcgg gtcagggaca tggctgatcg ggaggctgcc gaatcgaatg aaacccggtg
    25741 gattgcaaga cgcaacttgc catgaccgcg cataaggctc agggcgagag gtcatttttt
    25801 tggagcgctg aggcattgaa aacagcggac aaggccggat gcgaaggctg ttgccagcag
    25861 atattcattt gcacgccacg cggcattgtg atgaattgaa actgaatgcc agccccccca
    25921 agtcgaatca aacaagatca aaaaaaatgg ctcattgccg gtgtgctggc cgcagcaatc
    25981 ttggggggct tttggtcaat gtggctggct ggtccgccaa gcacagaaga tgattcgctc
    26041 gtggagcggc gcaaaaatgc aagcccgcca catttagaag gctcgacgag tggtgcgaaa
    26101 gatggccaga ctcggacgac atcgggcgag ttctcatcaa ggaactggga gtctggattt
    26161 cccaaagcgg gagaaagttc ggccagaatt cagttcatcc atgatcatgt ggcggaactg
    26221 gatgcggacc aggcgcgcgc cctgcttgtg gcggcgttgc gggatccgga ggcggatgtg
    26281 cgcatggagg cgctgaaaat gagcgggttg ctttcggagc gcgagataga caaagcggtg
    26341 ctgaacatcg cccttcaaga ctcggacgag catgtcaaag atcgtgcgtt cacacgggtc
    26401 aacgaactgc ctgttcgtga acggattcca ttttttggca atgcactgac cggaccaggg
    26461 gaggagactg cccgcaaggc ggcggaatgg ctgggcgttc tgggcgggtg ggatgcggct
    26521 gcagcgctgg tggctgcatg gcccgctgtt gcgcaccatc ccgctcgctc agcggccatt
    26581 cagagatccc tgcaacgcct tacgggccag cagttcaatg gggcagggca ggcggcagac
    26641 tggtgggcca agacgtcttc cgggttggac aaagacttgc tacccaagac gccatagcat
    26701 tctcaatttt gaaaataccc tgatgttttt tctggaaccc ttcgcctttc gtgatgacac
    26761 ttgacagatt catgatattc ctaatttgct ggtaaaaacc ccgacaatcc actagtctcc
    26821 cgacttcgca tgaaaacatc cctccctgga tctttgagca ctcgtattca atgcgccgtt
    26881 ttggccgctg gcctcgcgtt atccaccgca ccgtcatatt cgcaaaattc catcgtgctc
    26941 acgcctgtgg cggagacagg agaaaccgct ccaggcactg tctcaacgtg gagttccctc
    27001 agttcatcgt tggcctcagt aagcgcggat ggcggtgtca gctttgttgg gaccgctggc
    27061 ggtctcggcg gcctctggta cggcgaaccc ggttctttgc tgctcgcggc acgtcaaggt
    27121 gacgcagcac ctggaacggg cgcgaacttc ggaacgttca atattgatac cactgccttt
    27181 gcggcaactt ttgaggcggc gttttccaac acgctcgacg acagcaacga tagtatttgg
    27241 accaccactg taggtccagc tttggacttg gtcgccctgg aaggtgccgc cactcctgca
    27301 agtttggctg gcaaaacttt ctcgattttg aaccagccca gtgtgaacga taatggcgac
    27361 attgttttcc gagccacagt ggagccatct cccggggtcc gcaccctttg gcgcagcatc
    27421 aacggcgtgg gcattgagca tatcgccaga aacaccgagc caaccaatat cggggcgctt
    27481 ccaggcagca cctacaacgt cattggagac atgtattgca tcaacaatga tggcgaagtg
    27541 gtgtttcgca ccagtttgag cgggattgga gccaacaaca acgtgctgct ctccatgccc
    27601 gacccgatac ccggaggtgg ccccacactt gtggctcgcg aaggtgatgt cgctcccggc
    27661 gcaggtgctt ctacttacct ggacttcacg gcatgtcaca tcaacaattc tgaagatatt
    27721 ctgtttcggg ccagccttgg cggagtggct ccagcttcgg ccaacacagg cgcttggcat
    27781 tatgactctg ccacaacgac catcacggat atcgcggtgg aaggcaacgc aacacctggc
    27841 tttgtcgggg gctttttcga cacaattgaa gaattgttcc tggcggacaa tggtgtgatt
    27901 gttgtggtgg caatcgctcg cgacactgcc aatgacacag gcagtctgat cggccggggt
    27961 gtttggatgg acgccggtgc cggcatggaa ctggtggcgc tggttactca aaacattttt
    28021 gaccgtgatg gcagcacggt atatggcaca gtgaaaaata ttgaccaggt caccatgaat
    28081 tcggatggtg atattgccat tgagttgcga gctggtcccg gtgcatcctc catcaagggt
    28141 atctggaagc gggaagcagt tgcagcggct gtggcgcagc gtcttatctc ggtgggagac
    28201 accacttatg acagcaatcg cgtcgcccgt gtcctgaccg gacttgacct ccctgagtca
    28261 gaggcgttca cgtcgaacgg aggcggtccg attgggcgtc ccaggacgct tggagatgat
    28321 ttcattccgt tgctttgggc gaactatgcg ggtggcagcg gactatattt cgctgatgcg
    28381 cagtctctga gttttcttgt cacctccacc aacgaggagg cacccggcac ggctaatggc
    28441 actttgtttg attccctgcg tccttccgga accatcaacg aaaatgggtt gatcgccttc
    28501 cgtgctttct tgcaggatac aacgggtgat gctttggccg gtgtgaatct ccagggcatc
    28561 tgggcggaac tgccgggcgg gggcggcgtg cccacgctca atcttgcggc tcgtcagggg
    28621 aacatgactc caggcgggga tgtatatggg cagatgccgc tgaatccggt gttcaatgac
    28681 aacaatcaga tcgcgttttc ctcgccggtt caaggcggcg gcaacgcgct ctttgctgga
    28741 acacttgcct cattagctga gattgcgcgc tccggggatg ccgttggggc ttttacaggg
    28801 ctgccgactg gtctcgtata tgacttggtt cgctcacctc tggcatacaa caatggcgct
    28861 cgtttgctgg caagctgcac atttgccctt gatgaaatcc tgggagttac cgcgaacaac
    28921 gacagcgcta ttctccgtca cggaacctcg cgcaggatga ttgcgaggga gggcgacgcg
    28981 gtgcctgcgc cgcctgcgcc caatccggcc gccacgggtg tgttcgggca tctttacaac
    29041 gccaatgttt atctcaacac caatcacaat tccgccttcg tggcagccag gagagttaac
    29101 gccgcactgg gtatcaccag tgccaacaat gccggtgtct tttatcataa cggcaccact
    29161 ctgggcacag ttgcgattgg ctcgacggcg acaaacattc agactgcccc tggcaccggt
    29221 ggcgcgcaat atctgaagcc tgctgaaagc atctcgttca acaatgccaa ccgcattgcc
    29281 ttcctgacca ccctgcaagg cgcgggtgtc acactggcga ataatctggc cctttacagc
    29341 agcctgaacg gcgcggctcc cgtcctcctg gccaggacgg gatccaccca ggctagcggt
    29401 gaaggacctg gaggaatcgc tgcggctgct accttcgcgg cccttggcag acctcatcta
    29461 aattccacca ccacacttgt cttccgggcg aatctctccg tcggtgacgg tggggtgacc
    29521 gtgaatgatg atgcgggtat ctggagcaat tcgggcgctg gaacgacagc cctgctcctt
    29581 cgcaaaggtg attttgcacc ggatgcgaac ggtaatgcga ccgcagctgt gtttgactcg
    29641 tttgatcatc ctgtgatcag cgcggatgga cgcgtggtca tcggggccac gcttcgagtc
    29701 ggagtaggcg gtgtcactgt caatgatgat cgtgctgtct acgcgcagac atctggaggg
    29761 gtttttgttc ttgtggcccg tgaaggccag gcgcttgaca tcgccgatgg tcttggtggc
    29821 accgttaacg tcaacattcg cgacctttcc tatccaggtt cgggaggagc cgccagcaac
    29881 tcctacaagg ccatcagcac gagcggtttt gtcctcgtca accttgagtt cgtcacgggt
    29941 gagacagcca tgatgctctt ccttgtccct tgaacctgac aattgggtgg catacacctt
    30001 ttgtggttac cccggcgcat cttgattgga tgcgccggag cgctttaagg cgggctgatt
    30061 cgcgcccatg cgaaccggag tcccatgggc tggacccgga atcgagaatt ccatcaggca
    30121 attggggttg ccttcatgca gaagtttgaa ttttaggtgg aagccatcaa gcgggataca
    30181 gcctgctcca aagaaccggt tttttaaaac cgcagagtga cccgcgcgcg gaacatgcgc
    30241 ggctccaccg gatgcgcatg gacgccgttc acgggtgcaa gctcgctctt gagctggctc
    30301 tcgtaggcgt attcgatgtc gttgtcggcg cgattcagta ggttcaggca gtccagggcc
    30361 acttcccagt tgtcgcgccg gtagccgagg gtggcattga ccatcaggct gtcccggctt
    30421 tcgaactcat tggcctcgtc cagcggacgc gcgccaaagg cgcggacgcg cgctccggcg
    30481 aaccaccctg gcgcattgcc ctgcgctccc agattgatgc cgccgctgaa catgacgggc
    30541 acgctgttgg gaatgcggtc gtcaccgccc acgccgcgca ggcgcgcgta ggtcagggcc
    30601 agctcggcat cggcgctgaa ccaggaagcg gggcgccagt aggttgccag ttcgaccccg
    30661 tagcggtcgg agccggggcc cggttcgttg gtgccggcgt cgcccacata gagcagctcg
    30721 ctgtcactcc gtagccagaa gaaggcgagc gtggtggtga catcctcaat ggcctgggtg
    30781 cgcagtcccg cctccgctcc gagcgtgcgc accagggcat ccgcagggct gttggaggcg
    30841 gtgacgccgc gcgtgtcatt gctatggaag ccggtgccga agctcaggta gtactccgtg
    30901 tcatgccagg ggccgaagat ggcgctgaat ttggggctga cgatgccatc ccactcactg
    30961 ccctcctggt tgcgcgcggt gctggcttga ttgtcgaaat gataaaggtc tcccctcaac
    31021 ccgagcacgg tgcggaacca gggcgcccag cggctttgca actcggcata gaccccggcg
    31081 ctgttttgca tgaggtcgtc ctgccggatg atgctggtgc gctcgcggcg cgtggtgttc
    31141 cagaggccga tgccgtctat gaagtcatgc cgcgtctgag ccccgagggt gaaatcgtgc
    31201 tccacgccga atgcctcccg cttctcccag gtgcgcgcca tctggccgcc gaagatccac
    31261 cggttctcgg actgctcgaa ctggtctccc agggggccgt tgacgaagta gctgaagttg
    31321 gagaaaaggt tcagggagta gtggatgcca tagacattgg cgcgggtgac cacgtctccg
    31381 tcgcgggtct gcatggccac gttcaggctg tagcgctcgc tgtcgccgcc ggaggtgggg
    31441 tcgagtgtgt cgaagcggcc caggccggag gcgcgcacgg ggatttggtc cgtgctggtc
    31501 caagagccgg tgtagccctg gaaggtcacg gagaggttgt catcctcgtc acccgtgaac
    31561 cacctcagca ggccgttcca gcggttgaac tcttctggct gggtccaggg gccgtcatag
    31621 tagttgaact ccagcccgta ggtgagcgcg ccggcctcag tggccggcag cgtgcctgcg
    31681 agcagggagc ggtagtaatt gtactctccg aactcggcct tgatcatgtg cccgggcagc
    31741 cggtccacga ggcggaagtt ggcgctgccg gcggaggaaa ggtcgccgtc cgccgcggtg
    31801 taggtgccct tggcgtaggc gatgctgtcc accagttcgg ggatcacggg gttgaggtcc
    31861 gtgtagccct ggccgtgcgc gtgggagacg aagctcagcg gcatgccatc caggctgacg
    31921 gcgaagtcgg tgccgtggtc gaggttgaag ccgcgcagga agtactggtt ggctttgccg
    31981 ccgccggcgt gctgggtgat gaccatgccg gggatggtct ccaggatttc gccacgccgg
    32041 agcagggggc gggagaggaa atcctcctgg gtggcgacgc ctttggaggc ggtgtcggtg
    32101 acgcccagca agggccgtgc tttgccctcg acaacaactt cagggagcgt ggtttgagtt
    32161 tcggcggcgg gttcttgaga gcagagcagg ccgttgaagc agacggcggc cagaaagagg
    32221 gaggtgcgaa atttgaaatc tgaaaattga agatgtctga aaaaataaag gggatgcatg
    32281 agagtgggaa tgggttggtg ggtaaacgac ggggcgagcc agggggaatg cgggcacggc
    32341 atgccggcgg gcggcgcgcg ggcgcggcaa tgcgagccgt ctccgcagtt gcggaggcgg
    32401 gttcgatgtt gttcaaccca ctctcggtgg aggcaagggc ggcgcttggg ccagccttgc
    32461 aggcggcgga tcatggggtg atcccatttc cgcttggatg ggcgcgggcg ggaagaatcg
    32521 cggcgcgttc agcccgccca atgaccagtt tttgtctgcc ttgcacttgc tttttttggc
    32581 gggggcggaa gcaggcgctt cttccccggc gggcaggcca gcgcgggaga gaagcgccgc
    32641 gcattccgcg gcctccatgc gcgcgagagc gcctgccatg cgtgcttcca gaccgcttcc
    32701 gcgagtcagc cgctccagaa ccagcagccc gccgatgacc agcggcccgc ccagcacttg
    32761 gcagagggcg agcaggtaga gaagccgggc atttcgcttc agagcatgca tcggcatcca
    32821 ggctagagtg cgggtgccgg gtggcaagcg tgcgttggcg gatttatgaa agccgcggcc
    32881 tcggcgcaaa gggaggcaca ggagcgcgaa tgacgccttc aaagagccgt attgaaatgt
    32941 ctgccaggca tccgcctcat tctttggctc gcaaatcccg ccgcctctat gacaagacgc
    33001 gcggagcgcc tgcctcactg ccgccgatgc tggggcgatg aaggaaacga ccgcctgcgc
    33061 ccactgcgga tgccccgtcc cgcagggccg ggaggacggg ttttgctgcg cgggctgcgt
    33121 gtatgtccat gatcttttgc atgcgcaggg attcgaccat ttttatgatc tcaaaggagg
    33181 caatgccctg tcacccgtgc cgccacaggc cctgcgcgag cgcgactacg catggttgga
    33241 gcaactggcg gcgcaaacgg cgcaggctgg cggtgagacg gcggaactca ggctggctct
    33301 gcagggcatc tcgtgcgtcg gctgcgtctg gctgatcgaa aaaatcttcg cgcgccatcc
    33361 cggcggactg cgggtgaatg tggacgtggt gcatggcgag atgacgcttt tgttccagtc
    33421 cggtgttttt gacgcggcgg cctttgccag agacatgcag ggctttggct acctcgtcgg
    33481 tcccccgcat gaaggcgggg agcgcaagca gtcatccggc ctggagcggc gcatgggcgt
    33541 gtgcggggcc tttgccatga acgcgatggc attctcactg ccggcctatt ttggcatgcc
    33601 gccggacttc gcctttgccg cgtggttcga catgatcgcc gccgcttccg ccacactggc
    33661 gatgctggtt gggggctcct ttttcatcga aaaagcatgg cgcggcttgc aggcgggcgc
    33721 gctgcacata gacacgccca tagcgctggg catcattgcc gcttatgccg gctcgctggg
    33781 cggctggctg gccggagtgg agggcctgaa gtatttcgac ttcgtcgcca tcttcatctt
    33841 cctcatgctg gccgggcgct gggcgcagca ggccgctgtg gagcgcaacc ggcgcaagct
    33901 gatgcgcgac acctcgatcc cggagagcgt catgctgccg gagtccggca ggacggcgcc
    33961 gctgtccgcg ctcaagccgg gtgcgcgctt tgccatcaag ccgggacaat ccatcccggt
    34021 cgcctgccgt ctgctgagtc cgcgcgcttc cgtgagcttg gaatggatca atggcgagag
    34081 cgaggcgcag cagcgcgagg aagggcagat gctgccctcc ggcgcgctca acatcggcgc
    34141 gcgcgcgctg gaggcggagg cgctggaggg atggcaggaa agcacgctgc gccggctgct
    34201 ggaggcgcgc cgtccgggag acttccgcga cttgcgcctg gagcgtcttc tgcgctggta
    34261 cctcggcatc gtcatcgtca tcggagcggc gggcggtttg acatggtggt tgcgcggcgc
    34321 ggaggctgcc cgggcgctcc aggtgatgat ctccatcttt gtggtctcat gtccctgtgc
    34381 cctgggtgtg gcggtgccgc tggccgagga actcgcggcg gcgcgcgcgg agaaaagcgg
    34441 cgtgtttgtg cgcgcgctgg cgctttggaa gcggctgaca cgcgcgcgcc gtgtggtctt
    34501 tgacaaaacc ggaacgctta cgctggagaa cccggtgctg gcggacgcgc tcacagtctc
    34561 caagttgagc acggagtcca ggattgccct gcgcacactc gtcacaggca atctgcatcc
    34621 ggtgagccgc agtctttttg acgcgctagg ccccggcagg gtgcttgacg ctgaggtgcg
    34681 cgaggacatc ggcctcggcc tgtcgctcac ggatgacaaa aaccggattt ggagcctggg
    34741 acgctcaccc gcggctgatg cgcgcctggc ttgcgacggc gtgccactgg cggacttctg
    34801 ttttcgcgac cagatgcgcg ccgagtccat cgaggaggtt ggcgcactgc gcgcgcgcgg
    34861 tttgagtgtc cacattctca gcggcgaccg cgaggaaaag gtggccggca tcgcggcgcg
    34921 gctcgggttg gaaaaggagc actggctggc gcgcatgaca ccggagcaga aagcgcgctg
    34981 ggtggccggg cgcgacaggg atgacacgct ctacattggc gatggagcca acgacagcct
    35041 ggcctttgac gccgcccttt gcgcgggcag cccggtcaca gggcgcagct ttctggagca
    35101 aaaggcggac ttcttttttc tcggccacag cctgcgtttt gtcagcgctc tgcttgacat
    35161 cgcgcgcgtg catcagcgcg ccacgcggcg cgtctttgcc ttctcggtga gctacaacat
    35221 catcaccgtc atcgccggtc tcatgggtca tctgagcccg ctggcggcgg cgattttgat
    35281 gcctctcagc tcggtggcca cgctcagcct ggtggccttc accttccggg tgtcaaaggc
    35341 gggccgcaca cttccggctc gaaacgcggt ccggaggcag ggcggactcg aaggcagtct
    35401 cgccgccgct tgagcggagg gaggattcac cgttccggcg tgatcacctg ccgccccgcc
    35461 tcgaaggcga cgcgcggcgg tttccccgtc tgttcaaagg cgtcgtgcgc cgcgcgcaac
    35521 tcgctccaga aagagtgcca ctcggattcg cgcgccagct ccatgcgcgc atccgtcatg
    35581 cggaaaggga agacatgcac aggcacggca cgctggcccg cgtccagcgc ggcttcgaca
    35641 atcaaataga tctcctcaat gaccggatcg gtcatcgcca ggcagccgat gctcacattc
    35701 gagccatgca ccatgatgaa gctgcccgtg cggccgaggt ggcggtcatg cgcgttcggg
    35761 tagccgatgt tgaaggaaag atgaaaactg ctggcgggat tcagcgcgcc tctttccacc
    35821 gcgtaaaagc cctccggcac ctggccgtcg ccctcgcgct gcttcggccc gggcccgccc
    35881 gagagggcgg cgacgggata attgcggaaa agcacccatt cagacccgtc gcgcagccac
    35941 aactccagtt cgcggctctc cttgaaagcg cgcagataag cctgcgcgcc gggactggtg
    36001 ccgcgcatgg ccagctcgtc atgcaagccc ggcagcaggc gcgcgcgcgt gtccgcaaga
    36061 cgctccaccg ctgtgagcgc gctccatttc tccggctcgg gcggctcggg gtagggcagg
    36121 cgcagggagt caaacatcag gtggcgggcg aagcagtcta ggcagccccg cctctccatc
    36181 caaagccccg ccagggcgaa cgccgcagcc agcaggacaa ccaggaaaaa gatccgcgag
    36241 tgcatgaggc aagatgcatg agcaccgcgc gagtgcagcc gcgaaattca cccgcatggc
    36301 tcattcgcag ggtgcccgtg tcttccgctc caaagggaca tgcctgtcat gccggctggc
    36361 aggtgggggc cgtgaggctg agaagcgctg gatgataccc ggccaagcag acgcaccctg
    36421 cgccagtgac cctgccgcca ttttcactgt ggagtcctcc ggagtcggcg cctttcgacc
    36481 tcactttggg ctttcactac ccttgggatg ctgacttcct tctgagtaac ggttatcgca
    36541 taagctgcaa cgctgtcgct ccacatggaa ctggtggtgc cgggggccac atttttccgc
    36601 cagacgccgc tgttgaaaat gtggcgaacg gcgtggacta tttttccatc ccattggatg
    36661 acgaagctga ccggatctga cggcttcatt gatggctttg atcgtgcctc tgcactgcaa
    36721 catccacacc tgcgtcccgt tgacgcacca tgcgccgcgt tttccttctc tgtctcctcg
    36781 ccctcagctc tccgctcttc gcccaaaagg ccgcgaaggc caagaaagcc ggcacaccac
    36841 cacccacgaa gccgccgctg gtcagcgtgg acatcacgaa gctgccggcg ggcgcgacgc
    36901 atctcgatct gtggctgctg tgcgggcagt cgaacatgaa aggccgcgga ctcatgccgg
    36961 aggagccgaa gaacgacccg cgcatcgtca tgatgcacaa gatggacgac cagtggtatc
    37021 tcgcgcggca tccgctgcat ctcacgggcg acgcgaagac cttccaaggc cacgacaacg
    37081 ccggcgtcgg ccccggactg gcctttgccg aagtgctcgc cgcgcaggac aaaaccgccg
    37141 ccatcggcct cgtgccctgc gccgtcggcg gatcctccat caagctctgg cagaaaggcg
    37201 cgaagcttta cgaagacgcc ctccgccgcg ccaaactcgc cctgcagacc accgcgcccg
    37261 tgaaagcccg catccgcggc attctctggc tgcaaggcga ggccaacgcg aacgccgaag
    37321 aactccccca gcacgccgac cgcctccgcg cgatgatcga agccttccgc accgaactcg
    37381 cccagcccga cctgcccttc atcgcctgca ccgtcggcga gatgcgggat gaaccgcaac
    37441 tcacccacct caaagccatg aacgacatcc agctcgccct gctgaaaagc gtcccgcaca
    37501 ccgcctgtgt agatgcccgc gatttgaaga cacacatcgg cgacaatgtg catttcgaca
    37561 cggctgcaca ggaggagatc ggcaggcgct tcgccgcgga gttcaacaag ctggccggcc
    37621 gctgatcaag ccaccggttt gcgggcgagg ctgacggtga agatgccgaa gtcgtccacc
    37681 cactgctttt cctttttgaa gccggcctgc tcgacgagag cgtccatctc cccctgcgga
    37741 cggcggcgca tgacccagta ttcgccgtcg cggttggtga gcgtgcgcgc gatcatctcc
    37801 acctgaggat gccagggctg gcccgtgtaa atgagccagc cgccgggcgg cacggcgcga
    37861 aacagaccgc gcaatgagcg ctgcagccgc gcattgtcgg ggaagagttc ataaagcccc
    37921 gacaccacgg ccacggaggg ctttggctcc acagaggcga gcgactcctc atcgaaggca
    37981 tccccgcgcg catgggtgat gcgctccgcc agccccatct gactcgcgga ggcgcggccc
    38041 cttgcgagcg cggactcgct ccagtcccgg cacagcacat gcaaatcccc gccgcattcc
    38101 tcatccgcca tgacatcgag caggtagcgt cccgcgccgc cggcgatgtc catgagctgc
    38161 ggcggcatgc ccgcctcgcg cgcctgtctc agagcgtggc gcagcgcctg gttcatgcac
    38221 gccctcctct gccggatgcc gcgccagccg atggcattga tgtaaaagtg gtcaatgatt
    38281 tttccagcca gcaggttgcc gcgcgccttg ttttcataga cgtagtcgag cgactcaccg
    38341 gagtcgaagc cggtggcatg gccgatgcgg atgccctggc tgagcctgcc gagagtgcca
    38401 aggctggcgc gctgcaggat gaaggagagc cccttcaagg aaggcgaggg cagcggcttt
    38461 ttcagggcgg cgaactcggc gttcgtggct ggatggctga ggtccgcctc aagccgcggc
    38521 ggggtgaagt tctcgatgca gcggactgcg aagcgcttga tggctgcgca aacttcctcg
    38581 cggcatagat cgtggaaaat ggcgtggcgg gcgcctttga gtgtgagatg aattttctgg
    38641 tcgcagccgt agttcgtgta gagccggtca atagcccccc ttttcaccac cttgtcagcg
    38701 cctgcggaaa acaatagcag cgggcggtcc atgacctcgg cgtcgctaac gactctgcgg
    38761 gcggtgtcaa aaaggccggt gaggatgcgc gctgagatgt ccttggagat ggacgtgtcc
    38821 gcgtcgtatt cggcggcggc tgcgggatcc cgcgtgagcc agcttccgcg cacatagctt
    38881 ttgatggtgg cgccgggctt gagcttttgc agcgcgcgga tgccagtcag cgcgccgggc
    38941 acgatgaggt tcacctccag cgccggggtg gcgagcacca ggccagcgat gcgcggggca
    39001 aagtcatgca cccatgccga cgccaccacc gcgcctacgc tgtgcgccac cacgaccgtc
    39061 ttttccggca cgaggtcgtg ctgcttttgc aaatggcgga aaaacgtgtc gagatcgcgc
    39121 gcatactcca tgaatcccgc cgcgtgtccg cgccgccctg gcgactgtcc atgaccgcgc
    39181 gcctcccagg cataaatcgc ggtgtccggc atgagcagcg agggcgccac gctgtcccag
    39241 cggtcggcat gctcgtgccc gcggtgcagg agcaccagcg ctctggatgc cccctggacg
    39301 ttccaggatt tgtaatgcag ggactcgccg tcatcggcgg tgaattgaag acgctcggat
    39361 ttcatgagtg gattcggttg gagttggatg gatttgccaa taccgcgacg aagtccccaa
    39421 ggggccgcgc cgccggcgtc cgagcgctac tttgaatcca ttaacccggc gaatcaataa
    39481 actcgatgat gccatgcatg acgctgatgc acggcatgca ttcgacagat tcgtcagacc
    39541 acagctttaa caaaaacgta acccagcgcg ccggtggccg ggatggtgag cacccatgcc
    39601 cataggatgc ggccgacgac actccactgg atggcgttcc acctcctggc agcgcccacc
    39661 cccatgatgc tggtggtgat ggcgtgggtg gtggagacag gcatgccgag ggaggctgcg
    39721 ccaaagagaa cggtggcggc ggtggtttcc gcggcgaagc cgtgcacggg ctgcagtttc
    39781 accatcttat gaccgagagt cttgatgatg cgccagccgc ccgctgccgt tccggcggcc
    39841 atggtgaggg cgcacacgac tttgatccac accggaattt ggtcgccttt tgcgccgagg
    39901 gactcgatgc gcagaaagga aagccagcca ggcagctcct ggagatcacc cgcattggtg
    39961 ccggcgacaa gagcgagagc aatgatgccc atggtcttct gcgcgtcatt ctggccgtgg
    40021 ctgaagccca tgtagccggc gctgaatatc tgcatcttgc caaaggtgcg gctgacgaca
    40081 agcggacgca ggtagtagca gatgctgtta agcaggccca tgagaacgag tccgaaggtg
    40141 aatccagcca gcggggaact gaccatgggc agcacgactt tgtaaaagat gcccccgccc
    40201 tcataccagt gcttgccggc atcagggggc ttcgaccaga tgagcgcctc ccaattgccg
    40261 tcggcactgg cgagcgtggc gccgatcagg ccgccgataa gggcatggct ggagctggaa
    40321 ggcagaccga accaccaggt gatcaggttc cagacaatac cgcccgagag cgcgcagatg
    40381 atggtggtgg tggagacaaa ctggctgtcc acgaggcctg aggagatggt cttggcgaca
    40441 gcggtgccaa acagggcgcc gaccaggttc gtgacagagg ccagcaaaat cgcctgcctt
    40501 ggcgtgagca ccttggtgga gaccacggtg gcgattgagt tggctgtgtc gtggaaaccg
    40561 ttgatgtatt cgaaaatcaa cgcgaccagg acgacggtca ggacgagggt catgcgccgg
    40621 tgatcaggag tttttcaaaa cgatgtgggc gacgaccttc ccggcgtccc ggcagcgatc
    40681 caccaccttc tccatcagat catagagatc tcgcatcacg atgatggtga tggcacctcg
    40741 ggaggtctgg ctgtaaaggt cgcgcaggca ttccagaacg agcttgtcgg catcaccctc
    40801 gattttttgg agcttgtcat tgagcacctt cacgcgctca aggtgcagtt tcgaacgcag
    40861 ctccgtgatc atggtgacga cgcagtcagc ggccgagtcc atgagggtga tctggcggct
    40921 gaaatccgcg ccctccagat gctggcgcgc gatcatgacg cgctcggcga ttttctcgac
    40981 cgtcttgggt atcttgtaca aggcggtggc gagagcggag atgtcttccc gctccagttc
    41041 ggtgacaaag gtgcggcaca gagcctcgtc aatctgctcg gtgatttttt tgtcctcgcg
    41101 ccgtgaggct gcgaaagcct ccagcgttgc cccgtcgccc acaggcgcgg agagcaggct
    41161 gtgaagggac tggacgcttt tgcgcgcctg cgtggcgctg gcttccaata gatcgtaaaa
    41221 gacgtcattt ttgccaaaga gattttgcaa tgccagcatg gtcgggagtg tgtgagcggc
    41281 attaggagcg gaagtcaggc tggctgtcaa aaataacgat ccccgcattt gtgacgaaat
    41341 tgtcacaccc agactcgccc ttgtcttatc attgagtttt aaatcgcaaa tccccggatt
    41401 gttccgtgct cacaagccaa cttcgagctt cacacttccg ctctggcacc gtatcctcgg
    41461 ggtgatgttc tcgcgcccca cagccatcct gttctgtctc tccaccgcgc ttcatgccgc
    41521 gcccggcgag ctgcgctaca accgcgacat ccgccccatc ctgagcgaca actgcttcgc
    41581 ctgccacggg ccggacaaaa accaccgcga ggccgacctg cgcctcgacg tgcgcgaggc
    41641 cgccctggag atgaaggcca tcgtccccgg cgaccccgcc gccagcagca tcatggaccg
    41701 cgtccacacc accgacgagg acgacctcat gccgccgccc gagtcgaaga agactctcac
    41761 cgacgcgcag aaaaaaatgc tcgcccaatg gatcgagcaa ggcgcgccct acgagccgca
    41821 ctgggcctac acgccattgg tgaagcccga gtttccctca ttccgcaatc cacaatccgc
    41881 aatccgcaat cccacagaca ccttcatcca gtcccggctc gcggagaaag gtcacgcgcc
    41941 ctcgctcatg gccggcgcgc gcaccctcgc ccgccgcatc agcctcgacc tcaccgggct
    42001 gccgcccgcg ccgcaggagg tggagcgctt tgagaaagac ttcgcccagt ccccggatga
    42061 cgccgtcaac gccctcgtca cccgcctttt caagtccccc cacttcggcg agcgctgggc
    42121 cgtgtggtgg ctggatgtgg cccgctacgc cgacactgtc ggatttcatg gcgaccagaa
    42181 ccagcgcgtc ttcccctacc gcgactatgt gatccgctcc ttcaacgtga acaagcgctt
    42241 caaccagttc accctcgagc agctcgccgg cgacctgctg ccgcgcccca ccaccgagca
    42301 gctcgtggcc accggcttca accgcctcaa catgatgacc cgcgagggcg gcgcgcagcc
    42361 gaaggaatac ctcgccaaat accaggccga ccgcgtgcgc accgtcggcg gcgcctggct
    42421 cggcgccacg ctcggctgcg ccgagtgcca cgaccacaag ttcgacccct tcaccatgcg
    42481 ggacttctac tccctctccg cctactttgc cgacatcaag caattcggcg tctatgccag
    42541 ctacggctac accccggtgg aggaactgaa aggctggagc aacgaccacc ccttcccgcc
    42601 cgagatcgaa gtggacagcc cctatttgaa gcagcgcctc cagcgcaccc gcgcccgcat
    42661 gatcgagacc gccgcccagg catggaagga cgccgtgaaa accgactcag gccgcgcctc
    42721 catccagtcc tggatcgaac aggcgcgcgc gcatctccag gcgcatcccg aaggctggct
    42781 cacccccgcg catgaggccg ccacccggcc cgcgaaggag gacaaaaaag ccccgcccgt
    42841 ccaagccgcc gtccagcccg acggcagcgt gctgctggaa acccgcgcgc cgcaaaacac
    42901 cgtcatcacc ctcaaacccg gagccgcccg catcgccgcc atccgcctgg agctgctgcc
    42961 gcacgaattc caccgcggca tcgaaatggg cgccgccacc aacggcctca cgctgaaccc
    43021 cgccttcacc atccgccagc ccggcgcgga caaggagacc ccgctcgcca tcctccacgc
    43081 cgacgccccg gaaaagcagc cgcgctactc cggcactgag gaggtcatcg gcatccgcgg
    43141 cggctggaag acctccgtgg ccttcgcccg cgccgcgcag cacagcgtgt ggattctcga
    43201 ccaggccgtc gagctcgcgg agggcggcgc gctcatcctg cgcttgaaca accacagcgc
    43261 cggctgcctg cgcgtctcca tcagccctgt ggcgcccttt ttgcccctgg agaaaaactg
    43321 ggccgccgcc ctgccgccgc ttctggacaa agccctcacc tccttggaaa agaccgtcca
    43381 cccgcgcatc gccaccctct ggctcatgca gtccagccag gctcccgcct ccgcgcggga
    43441 aacatggcgc gcgctctatc acgaggcgct cgcctgccgc gatggcaagg ccatgaccca
    43501 ggtcaccgtc gccctcgccg agcccatgcc cgtgcgcgac ctgccgcgcg gaaactggat
    43561 ggatgaaagc ggcgccctcc gcccgcccgc accgcccgag ttcctcaccg gccccctgaa
    43621 gccggacgca ccgcgccaga cccggctcga cctcgcccgc tggctcacct ccgcggaaaa
    43681 tcccctcacc gcgcgcacct tcgtcaaccg cctctggaag caattcttcg gccacggcct
    43741 ctccagcgcc gtggacgacc tcggcgccca gggcgagacc cccagccacc ccgagctgct
    43801 cgactggctc gccagcgaat tccgcgacag cggctgggac atccagcacg tcatgcgcct
    43861 catcgtcacc agccacacct acctccagga cagccgcgcc cgccccgaac tgcgcgaggc
    43921 cgaccccgac aaccgcctgc tcgccttcca aaacccccgc cggctcgacg ccgaattcgt
    43981 ccgcgacaac gccctcttcg ccgccggcat cctcaacctc gacctcggcg gccccagcgc
    44041 caagccctac cagccgcccg gttattacga caacctgcaa ttccccagcc gcgactacat
    44101 cccccacacc gacgaccgcc agtggcggcg cggcctctac atgcactggc agcgcacctt
    44161 cctccacccc atgctggcga acttcgacgc ccccgcgcgc gacgaatgca ccgccgcccg
    44221 caccgtctcc aacacccccc agcaggccct caccctgctc aacgacacca ccttcgtcga
    44281 agccgcccgc gccttcgccc aaagcctgcc cgccgcttcc gacgaggccc gcctcggcca
    44341 catctaccag cgcgccctcg cccgcccgcc caccgacaag gaacagcgca gcctgcttgc
    44401 cttcctcgcc acccagcgcg ccgccttcca agccgccccg caggacgccc aaaaactcct
    44461 cgccaccggc ctccgccccc tcccggaaaa agccggcccc gccgaactcg ccgcctggac
    44521 cagcctctgc cgcgtcgtcc tcaacctgca cgagaccctc acgcggtatt gatccgcgcc
    44581 ggcaaagggg gaaaaattcg aatatcgaaa ttcgaaattc gaatcagaac ttcgtatttc
    44641 gaacttcccc ccctccccct caattcccct tcagcgccgt tgcaaatgtc aggcatcccc
    44701 ccgctggacc aggttggcat gataaataat tgattttcag tgccttgggc gcatccgcga
    44761 cggttcgggt gcgtgccaga caagggtcaa gagaatgatc cagttggcat tttgggtgat
    44821 tcagaatgca ttctggccga cccagcgcgc attctgaccg acccagcgtg tattctggcc
    44881 ggcccagcgt gcattctggc tgacccagcg cgcgttctgg ccgacccagc gtgtattctg
    44941 agtgacccag catgcattct gagtgaccca gcgtgcattc tgagtgaccc agcgcgcatt
    45001 ctggccgacc cagcgtggat tctgtgtgat ccagcatgca ttctggccga cccagcatgc
    45061 attctggccg acccagcgtg cattctgagt gacccagcgc gcattctttg cagattgcag
    45121 gggttacaaa tgcctggcgg gaatggcact cagttaagac aaggtgcggt gccgtaggcc
    45181 gggtgtgtcc ggcgaagaat cgcgccgctg aactcaacaa gccctcaccg ccggaccgcc
    45241 cggcttttgg cgcgtgaatc ccatgccgga ggtgcgtgca cagccgggcg ggctaaagcc
    45301 acacccggcc tacgaatcgt cattccgcca cgattcagcg ccttaactga gtgccattct
    45361 tgcccgccag cttttcagct tttccttcgt gttccttcct gaaactgcac ggcagacggc
    45421 agatggcatc ttcacaatga gatcagaatc ggcatgggct ggagtgatga ggatgcggac
    45481 agcgcagtgc agcattctgt ccttgcctgg ctagccgacc aaggcatcat cagatatgag
    45541 agggttcccc gaagatgatc cgacgaaaaa atggctatga aagcgcactt tgattttgac
    45601 aagcacaagc atgagctgag gcggatcatc caggacacca gggccggcag aatggacgag
    45661 gaagccgctg tcaagcagct cctgaatcct ccattcagga tggccggcag gctggaggcg
    45721 ctgagaatgc tgcgcgtgca aagagcccag cagggttgac cccgctcccg ccgccgcatt
    45781 ggtccttgga gcttccccct gtggcctaca ccctcaccca agaccagatc agccccagcc
    45841 ttgacgcgct ggccgaaccc agcctgcgcc tgtcatagat gcccggcagc tttattctgg
    45901 cagctttttg cgggatcttt aaaaggcttg agatgggtgg ctgagacgct atcgggatgt
    45961 catgggaaga atcgaagcct atctgaaggg caaactcatg tttgtggctg aggccgagca
    46021 caagatcgag aaggcgcaca ttatcttcga ggacagagaa ggattccctg acctcagggc
    46081 cagtgagcgt tcctgccttc tgggtgttct ttcttttgcg cagaagattg ccacagaggc
    46141 tggcgtaaag cttgagctga agcagaccgg ccatattggc ggcatggtct attgatcccg
    46201 ctccctctga gcgatttccc ctgaacgtca ctcctctccc caaaaggggc tgaatttctc
    46261 gtatgccacg tcaggcagcg gcagcggctc cttggccgag gcgcggaact cctcgaagag
    46321 ggcgagagcc tcttcatcgg cttcggggag ttcatcaaga atgtccatgg gccaagccta
    46381 acttcatgag ccttcgagtc aaccgccagg accatgacaa cgccacggcg gaggcgcttg
    46441 cagagaccgg catcctcttt gaggagaaaa atggcccggc ttcccgatca aggtcaactg
    46501 ggccgcgacg aagacgggaa cggagcgcta cccccggccc tacgcacccc gccactacat
    46561 agcgccgcga cgggatggtt gcctcgaagg ctttcaaact ggcatggcgg gcgtgaagat
    46621 caatcgcgga ctcagcaagg gagccttgcc caaaggatgc cgtgcaactc ccatcttgat
    46681 tttcccccgc cctggcagca tcctcgtcac gccctcgctg aatgtcggca atgacacaat
    46741 tcaccgtgct gggttgggcg cgccattgcc ggcgcgccca actgtttttt gggaagcctg
    46801 gcgcctgccg cactttggca aacgcgccta cggggcaaag taggctcgtt cgccagaacg
    46861 aggggtttgg gggcgggcgg gagtcaagcg ccttccgcag tctggcgact gcgcctacgg
    46921 ggggcgtcac ggccagcggg gcggtgagct gctcgtagag ggtgaagagg tgctcgacgc
    46981 gggcgcggtc gttggggaag gggtcccggc ggtagcagcg gtccacggcg cggcactttc
    47041 tcagatgacc caatatcaac tcagccatga taagtcacgc ccaatcagca gctccttcat
    47101 ttctgcgtga tgggtgtttt tcatggcgac aggtttgcat caactcgcgc acctctgctt
    47161 cactgtttcg tgtgctaatg taatcgcaga ttaacggtgt ttaatattgc ttgaatattc
    47221 cctccggaat tcctcgtaca caatccgaag cactctggct ctgtcttcga catcttcaaa
    47281 ctgaccagct aaacgcagga gatcatctct gctgattgac tggaccttca tgcttatcag
    47341 gtattgcttc acatctcggg aaatgtcgtc aataaccagg atcacattgc cttcgtgcag
    47401 tcgttcttga tcaaaattct cttttagttc agtataaacc gatgtggcgg ccgcttcagt
    47461 aagcagcttc agtttcttaa tttgataaac aactccaaag tttgtcgaaa gatcaactcc
    47521 gccatcatgg gatgacgttc gcgtatcccg atataaccga caagcaaact tgtctagatg
    47581 aaccttgaga acggcgaagc taaagatttc aaaattggct ggtgttttgt tgatatgctg
    47641 tgtcagtagt tcctggaagc tggcggcaga actcgcgaac tccaaccttg ttctagcatc
    47701 gtgaaattgt ggcactagtc caagcctcat cgtaatgtgg gcctttattc gttcgcgtat
    47761 ccatttacaa agttccggcg cgaacttgag atagggatca agcaactcat aagtcatggc
    47821 agacactgtc ttcagaactt gataacgagg aagatttctt gatggataag catcataata
    47881 ttttccaccg atatggacat catgccccaa gaattcgatc aattcatgaa cggtcttttc
    47941 gtaaagtctt ctaatttcac catctgtaaa gagcgtcttc ccggacgccg tatattcttg
    48001 gagtaacgcg agagtgataa ctggaaaaat cactttagtt tgcgaatttt ttagcatccg
    48061 atcaatgctt gatttgcaga attgctcagc ttcttgagtc gtcatgatta ttcaattctt
    48121 aaacagcaac caagtgaatt gcttcttcca agtgtttaat gaaagctgac agcggttcaa
    48181 ttgaatgtcc gtcagatact tctgatcgaa tataaactgt ccgcgtcagc gtaagctggt
    48241 actgcccgtg atagttcgtt ggcggcagca tgttatagat gagaagagcg aagtcggact
    48301 cttctcgcgg gacacagggg atttcgggaa gcgtgcgaaa gaaggcttcg tctatggcta
    48361 cagcagactt tttcttccag gcgtgaagaa tgccgccctt gaaaagcaac tgcggagcga
    48421 ggcgcttgcg cgaagatgag aggtagtcgg gtcgtgggta gttgggttct tttgtccaat
    48481 ccatgtcggc tagcgtctcg ggggcggaca tgaattgagc gaatggattg cgcacgttgc
    48541 ctgagatata aacggcctgc acctccagag cgccgaagtc gtaaacgcgt ccttgctcat
    48601 cataagcaca tagcaccaca tcaatgttac cggctgaaca tccgtcagca tccttcaaac
    48661 ggacctctgt gagggtcgtc caatttgttc ctggcgggaa aaagaaatct gctgcgtggt
    48721 cagcgatgag ccagtcttgc ctgaaacgga ccggacatgt gatggcaagg tctttgccat
    48781 gaatcacact gcaaacgccg agagggtctt gagctttgtc tttggtgcag ttgggaacct
    48841 tgttgttgaa ggggcaaagc cgtttgctgc gatgtcgctt ggcagcggcg tcatttgcgt
    48901 ccggagggaa gccaaagact tctgccagag gatgcttctg ctgagaactt tgaggcatgg
    48961 tggcgagtta agcgggtagt gccagaattg ccagcctgga tgaaaaaaaa tcggggtgaa
    49021 tgcggctcca acgaaggctg gtaattctga ggagcattgc gcgtattgcg gcatcccagt
    49081 cagtggccgc gatgttcgcc gggtcttcgg ggtcaagccg gtgctgcttg tagccggcga
    49141 tgaaggcgaa gcggcggatg tgcttgtgga ggtcggtgac ggggaaggtg agggattcgc
    49201 cgccgccttc gagatcgtgg atggcgatgc gctggaagtc ggagacgatg aggtggcggg
    49261 ggatttcttt ttcacgaccg gtgtcgagca ggccgcgcac gtaggccatg ccctgggcgt
    49321 gggctttgtc gaggtcctgc ccggcgcttt tgtgctcgcc gaggagtttg ccgggccaga
    49381 agacatcaat ggcgccccag ttgccggaga gttttttcac cggctcctcg aaggaggcga
    49441 cggtgcggcg gcggatgccg aagatgtcga agaactcgtt ccagaaggtc tggcgctcgg
    49501 cggcctcgcg ggtctcggtt ttccagtcgt tgccgaactt gatggcgcgg tgcttgattt
    49561 cgttccagga gaggggcatg gcgggcgagc gtaggcggag aaggcgggct gggaaggggg
    49621 aagtgcgcgt tcgttgtcca ggcttgcgcg cgctgaagaa gcgggcaacg cggggtgagg
    49681 cgccgccaaa atacccgctg gcggagcggg aggaagcatg tttcttgcgc ccatgaacca
    49741 ggagccgccg caaccgccgc cgcagcgctg tgtgtgtgac cgcgaggaca cgggcggctg
    49801 tgcctggatg ctgggattgc tgatgttgcc tggagtcgtt gtgttgttgt atcaggcgct
    49861 ggtgcttttg ggcctggtgc gggatgcggt gatgacggcg gaattcgccg tgctgctgct
    49921 ggcggcgtgc ggggcgtggt gtctgacgct gctcttcagc gggccggagc gccgcccgct
    49981 gggctggctg ctgctggggg cctttgtgct ggccggggcg gtgttttttt tcattctgcc
    50041 gctcaagttg ccgcgcctgc cggacggcat ggtgctgctg gttcttgcgc tggcgggcct
    50101 gggggctttc ctcgtcgtgg cgctgcgtgt gctgtcggga aattgggggc ggtgaagccg
    50161 cacctccgaa agcgcggatg cccggccgat gccttcgggc atttgctgcc gtggtttcct
    50221 ttttccgtcc gctccgggct gccagacaac ttccgcggct tctgtgcttg cgcccccccg
    50281 gtgaagcgcg cgctggcagc atcacccgcc ccgccgccgt agatttgggc ggctgatgaa
    50341 gctgctcctc ctcgcctccc tcatccccat gccggcgctg gccgctgaat acgcgctcac
    50401 ttttgagcgc gatgcgcgcc cgatcgtgaa ggcgcactgc acgcattgcc atggcgagg
//
LOCUS       DAOMYJ010000007        48787 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Prosthecobacter sp. isolate AMR_MDS_5629
            NODE_280_length_48787_cov_21.871460, whole genome shotgun sequence.
ACCESSION   DAOMYJ010000007 DAOMYJ010000000
VERSION     DAOMYJ010000007.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867850
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prosthecobacter sp. (wastewater metagenome)
  ORGANISM  Prosthecobacter sp.
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Verrucomicrobiaceae; Prosthecobacter.
REFERENCE   1  (bases 1 to 48787)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 48787)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.30
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:34:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,742
            CDSs (total)                      :: 4,684
            Genes (coding)                    :: 4,676
            CDSs (with protein)               :: 4,676
            Genes (RNA)                       :: 58
            tRNAs                             :: 55
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..48787
                     /organism="Prosthecobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_280_length_48787_cov_21.871460"
                     /isolate="AMR_MDS_5629"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1965333"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..745)
                     /locus_tag="PLP58_01365"
     CDS             complement(<1..745)
                     /locus_tag="PLP58_01365"
                     /inference="COORDINATES: protein motif:HMM:NF014841.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-2-hydroxyacid dehydrogenase"
                     /protein_id="HRJ07200.1"
                     /translation="MKIVLLDAHTANPGDVSWAPLDALGTCEIHPRTPVDETVARCAG
                     AAAVITNKAPVTRAMMEALPGLKYIGVTATGYNIVDTAAAKERGIVVTNVPGYSTPAV
                     AQLVFALLLELTNHAGHHARTVRDGRWSACPDFCYWDHPVIELGGRTLGVIGYGDIGG
                     AVARIALAFGMKVLASRREWKTPPPEGVQPATTDEIFRQSDAVTLHCPLTDATKHLVN
                     EGTLGLMKPSSFLINTGRGPLVDEAALAAA"
     gene            1139..1804
                     /locus_tag="PLP58_01370"
     CDS             1139..1804
                     /locus_tag="PLP58_01370"
                     /inference="COORDINATES: protein motif:HMM:NF012264.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HRJ07201.1"
                     /translation="MKYFFLSYLFVAAIVVAAFGTRGSKSELPPIEILPDMDYQQKVK
                     YQAKSDFFADGLGARRPVAGTVPMGFEVPAKPASSPEYQPPAFGFSHGTGYYNTGKFG
                     DYYGDGLPKEIELSKSLLERGERQYNIYCAVCHAASGNGKGVTSKYGILTAFNFQQPG
                     SLDPANAAAYRADGAMFDVISNGKGLMGPYGGSISVQDRWAIVAYIRAMQVAVKENNV
                     SVQ"
     gene            1823..3124
                     /locus_tag="PLP58_01375"
     CDS             1823..3124
                     /locus_tag="PLP58_01375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ07202.1"
                     /translation="MSHHVTFKDLPEGGEKFDTAKGAKMINAFGAAAAIGLAGSGYFF
                     FSRTGEFAYSWLFAFFFAFTVVAGACFWILLHSASNSGWGVAIRRLWEQLANMILPLA
                     VLGLPLLLVPTVQDSLYEWMEHHREAAHHAHEKGESVADALHHMAEGNSHLHILVDKY
                     GYLNMWFWKFRFVFYFFALWHIASTLRRKSVLQDEDGDIKHTVKSRTFSCRWLLFYAL
                     TVTFAAVDWLMGLDYAWFSTMWGVYIFAGCAWSSMAVTILLVNWLKSIGHLKIVTEEH
                     YHLMGKLLFAFTVFWAYIAFSQYFLIWYANITEETRFYLIRNTEGWRWVSIFIVVGHF
                     IAPFVLLLSQPRKKNPALIGLICLWVVFMHLVDIYWNIMPERGPSLAIGLMAPGAWVG
                     DVFALLAVVGTLGFLFLRSLGKHSLYPCRDPRLLESANVVN"
     gene            3131..3511
                     /locus_tag="PLP58_01380"
     CDS             3131..3511
                     /locus_tag="PLP58_01380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ07203.1"
                     /translation="MSPSDTSPKAGATFQWVLGAFASFAVLFWVIQTFFVTPGDADPR
                     AAERLANSAEISALQKDVLAKMGLSDPAKKAAVFDKTLQVLKTRPESASGQVVPGSPT
                     QLKQAAEAPPAAGAAPAPSATPQN"
     gene            3532..5013
                     /locus_tag="PLP58_01385"
     CDS             3532..5013
                     /locus_tag="PLP58_01385"
                     /inference="COORDINATES: protein motif:HMM:NF012343.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0009060 - aerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome c oxidase subunit I"
                     /protein_id="HRJ07204.1"
                     /translation="MQTTPATTDTQADDLRRAAIDKSVKGPVLFLFLNGAFWLMASTI
                     LGVLAAIKQFAPEFLGACSVLQYGRLQPTHVNALVYGWGVQAALGAMLWIMARRTGQE
                     LKSGKSILFTAAIFWNIAVTLGLLGILLGQSTSMEWLEMPAFVWPVMLASFLAFAWPL
                     ARMFGRAFRPDGFMVSTWYVLGACFWFPWVFITANVALHCLPGLGAIGAGVNAWYVHA
                     LILLFFAPVAIGAAYYFIPKITGQPIASAQLAKLGFWLLAVLGGWTGMQHFMGGPLPA
                     WMVAVGAAAAVLLLIPVGVVGLNHHLTTLGKHSLVASSPTLRFVFFGSLFYPVSGVIL
                     ALISNFNTGATLQFTQAWYGFQMVAVYGFFSMTLFGAIYYIVPRLSGCEWLSVRLIRN
                     HFWFSVYGTATIVVTSLVAGVQQGADLNHPEMWDQDFSQLALNLRPYIVARALAWGLI
                     TWSNFWFLIHLMLMVAGLGRRSSAPTLLAHDHEDEALPNAAHA"
     gene            5010..5684
                     /locus_tag="PLP58_01390"
     CDS             5010..5684
                     /locus_tag="PLP58_01390"
                     /inference="COORDINATES: protein motif:HMM:NF014486.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome c oxidase subunit II"
                     /protein_id="HRJ07205.1"
                     /translation="MTQFRTFILALTASFGLPWLCLIVVPAVKYQALSPLPYDKEADG
                     MDGYYPPAPINRQGQLVYAREGCAQCHTQMIRPPQIALDGWRKGWGQDQEGRPPEPVR
                     SNELRDYHGEKFAFLGIQRSGPDLANYGWRVADRNAVHQKLFAPRSIHDWSTMPPLRH
                     LYTVRLAQGQPSEKALKLTGKHAPKPGYEVVPTEDAEALVDYLLSLKKDYPIPGRAAA
                     MAAAAK"
     gene            5716..6447
                     /locus_tag="PLP58_01395"
     CDS             5716..6447
                     /locus_tag="PLP58_01395"
                     /inference="COORDINATES: protein motif:HMM:NF012264.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HRJ07206.1"
                     /translation="MSAPNSTVNTDSTDLDRLHAAVRREKADLEPGREPVPMWIMFVF
                     MLIAIIAGGQLGQMTGGWSFDVSNPWSLALQGDPRPGGDGGAGALDPFQTAMKKGASG
                     YAVCGGCHQGSGMGIPGQYPPLAKSEWVTGGTERLIRVVQHGLVGQINVAGQNYNFPG
                     GMQPFGAAMSAGDLANVLTYIRNSWGNQGTMITKEMVEKVRADESSRAAQWTQADLEK
                     FAEANVPGEIPAGPGATAAAPAEAK"
     gene            6471..7295
                     /locus_tag="PLP58_01400"
     CDS             6471..7295
                     /locus_tag="PLP58_01400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase regulatory-like
                     domain-containing protein"
                     /protein_id="HRJ07207.1"
                     /translation="MKQAWQSSSDRASLTLLAAWLLSAAVSCGKAPEAGSSYQPPPAV
                     TVAETPSITGRVVLTGGLPSRAGKKLDVAGNPFCTGHGDLMDPAWKVSPEGGLADVVI
                     TVKSGQRAANVPDFAGLVDQKNCEFAPHVTVIQAGQGLRLRNSDLTFHNIRIARHELG
                     TRDRGVNLDNLAQAARGDENSKVFHEPGIYRLECDVHRWMRAWVLVHAGAHTAVTDAS
                     GDFTVTRALPDGDHVVEAWHPMFPEKLTSTVTIRDGRAEASFNFDLAQSFEPPADR"
     gene            7326..9281
                     /locus_tag="PLP58_01405"
     CDS             7326..9281
                     /locus_tag="PLP58_01405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007418871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0009060 - aerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome c oxidase subunit I"
                     /protein_id="HRJ07208.1"
                     /translation="MSAAATTHDHSHGADHAHDHHEPGFIGKYIFSSDHKVIGIQYAM
                     SGLLFLLFGFFLMLLMRWSIAFPNEPIPGFAFVRSLPFIGDFLFHNMIGRWAPNGVVN
                     GELYNMLGAMHGTIMVFLGIVPLGFAGFVNFVMPLQIGTIDMAFPRLNMWSFWLFFIS
                     GVMMLASFFVGTGSVQTGWTMYSPLASTTTLAVTNIWMHGHTWWLLAMVFNISASLLG
                     SVNVITTVINLRARGMSWMRMPFFCWAMFVTAFLLLLAFPPLEVAALLQLVDRVFGAS
                     FFLPTGLIEGGQHLDIAGGGSPLLYQHLFWFLAHPEVYVLILPAFAILTEVIPANTRR
                     PLWGYKSMVYAALTLGFLSFLVWAHHMYLTGMGTMMSTYFQITTVLISVPSVILLTGL
                     MISLWGGSIRFTPQMLFASAFLPMFGIGGLTGLPLAFIFIDLALHDTYYVIGHFHYVV
                     APGTIFALFAGVYHWFPKATGRIMSDKLGKLHFWPSLLGMNLVFAPMFIQGMGGFHRR
                     WYDGGANFKQTSEVSNFWTELNSNLFGFFGMEVPNNLLGLNVLMSVGAFILALGQVPF
                     ILNVFLSIKGGKKVASDNPWNCTTLEWATPTPPPHGNFTFEPVAVRGPYEYSAPGIDQ
                     DFFPQWESEPGKAATAPASSSAAVAHH"
     gene            9318..10781
                     /locus_tag="PLP58_01410"
     CDS             9318..10781
                     /locus_tag="PLP58_01410"
                     /inference="COORDINATES: protein motif:HMM:NF012720.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit 3"
                     /protein_id="HRJ07209.1"
                     /translation="MDIPYTVSARPDTGLYNAKVGIWLFLASEVMLFGGLFSSYIFLR
                     VGADYHWPVHELNVFLGFVNTLVLIASSVTVVMAWASLKLRQIGKFKLYMAITVLCAA
                     IFMVNKAFEYKAKFEHYAVKLTDGTFLTGHLPQGYEIKFGDVSEVSVLIAGENKAVDG
                     EPLKHFVPRVEGDLPAFKMTDGSSITLDQSGFTTLKEATLKAAREKLEEDRKKRREEG
                     GAFIEAGNIAAGKAKLDEAARMNIVPDTTIKLKPEQPVTVKVKPSQIFSYTANSLTFK
                     DGTTLTGKLLDDSMVLEVDGVDTRSVPDAEKSLAWKSGLLGEPWKKAFIANRDHHLNA
                     FEEKHGDRRDPKKSATLQKEAFFLKIKSATPDARDDAHGNSKSAAASAPADSHADTGH
                     HGHHPVVHVDRKDIAFYSNYTPKLNTYYAIYFTLTGLHGLHVVAGAIVLFYFLVFDGR
                     RLRENPEHLANRVEVGGLFWHFVDLVWIFLFPLLYLL"
     gene            10794..11105
                     /locus_tag="PLP58_01415"
     CDS             10794..11105
                     /locus_tag="PLP58_01415"
                     /inference="COORDINATES: protein motif:HMM:TIGR02229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome C oxidase subunit IV family protein"
                     /protein_id="HRJ07210.1"
                     /translation="MADSPEEIQKSIRKYLWIGGILIFFTVVTVGLSYVELPTHGLNI
                     LVGMIVATFKAALVALIFMHLNHERILIYKVLAFTTVFCLALFLLFIFSAGDPLVFEG
                     F"
     gene            11128..11334
                     /locus_tag="PLP58_01420"
     CDS             11128..11334
                     /locus_tag="PLP58_01420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ07211.1"
                     /translation="MSLKHFHMVFIFFAILCDLGFFVWTRLLPEKAAQLGVEELGMLA
                     GWLSLALTGYGVWYVVKKSRRIII"
     gene            11362..11538
                     /locus_tag="PLP58_01425"
     CDS             11362..11538
                     /locus_tag="PLP58_01425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ07212.1"
                     /translation="MNFLACATCRPDPGSLIAQAQDMAVLVMLGFLMLGMGAVGLIVM
                     NFARKQRRNPTLEA"
     gene            11558..12346
                     /locus_tag="PLP58_01430"
     CDS             11558..12346
                     /locus_tag="PLP58_01430"
                     /inference="COORDINATES: protein motif:HMM:NF012344.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit II"
                     /protein_id="HRJ07213.1"
                     /translation="MSVSDINHWFGITQNASEHGGLVDHMLGFVHWFMLFLFIGWSIF
                     LVIAFTRFRQSKNPKADYHGVRGHASTHIEIGVVIVEAILLLGFAFPLWSRQADDFPT
                     SPDTIKMRALGEKFLWNFHYAGNDMMLGSWDLRLVSSANQVGRELQDPNGKDDFVNPG
                     TMRLPVNRPVIVDVASKDVIHNLAIVPMRAAQDACPGVKSHMWFKPVRTGTWDIICGQ
                     LCGPGHAQMRAVLEVLEGPEFDEWAKEQSASAAAKSASPVAAAQ"
     gene            12429..13514
                     /locus_tag="PLP58_01435"
     CDS             12429..13514
                     /locus_tag="PLP58_01435"
                     /inference="COORDINATES: protein motif:HMM:NF014665.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_process: GO:0006784 - heme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="COX15/CtaA family protein"
                     /protein_id="HRJ07214.1"
                     /translation="MRAFHIQSLFTLAVGVLLVWWGAAVTTEDVGLAVPDWPLCFGRL
                     NPEGWYKVPALLLEHGHRWIATFIGFQVLAMYFWQFAKCQPRFIEAAGIIITGVAYLF
                     LVFRQALAPAGVILFLGLVWLVLNWIGQRWTLLRGLTTAALFLVIFQASLGGLRVLKM
                     SDPYGISHGTTGQLFFCLLVLVALASSRVWCSGGLRMGWHDRKKARLLGSLLFGAVSM
                     QLVIGAILRHTQRAHLAANDILTTKGMLLPPFDPADVFVLFLHKYWGFCVAGMVLFVA
                     WPARRWFSAIPGLRVVPRLLLIMPLVQVALGVAVIWTGKSFWYTNFHVLNGLGLLVCA
                     FLMMAGAWGARLIPEKETPAGSLEAAA"
     gene            13602..14498
                     /gene="cyoE"
                     /locus_tag="PLP58_01440"
     CDS             13602..14498
                     /gene="cyoE"
                     /locus_tag="PLP58_01440"
                     /EC_number="2.5.1.141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018970321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008495 - protoheme IX farnesyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006783 - heme biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme o synthase"
                     /protein_id="HRJ07215.1"
                     /translation="MMNDLLVLTKFRLSALVIVTTFVGFWLRRGPDWSGWLLFHTLLG
                     SSFAAFGAAVFNQLMEIGPDSRMTRTADRPLPSGRISPGAAFGIGWLLSAFALIHLGA
                     KANIEAAALTALTLAVYLFIYTPLKKQSAANTLVGAVSGALPPLIGWAGAAGQTPADA
                     RYFRWELLLEPGALYLFALLFLWQLPHFLAINWMYRDEYRRGGFVMLANEDEEGVRTS
                     RHALAYSLATLLLMFYPVARGLVNPWLFLPPALLLAGWLCKLALVFQKSPDRVNARKL
                     FFCTLAYLPGSLIVTCLAWKHG"
     gene            14539..15255
                     /locus_tag="PLP58_01445"
     CDS             14539..15255
                     /locus_tag="PLP58_01445"
                     /inference="COORDINATES: protein motif:HMM:NF014667.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCO family protein"
                     /protein_id="HRJ07216.1"
                     /translation="MSDQTRPFNPLAIWIPIVMMALGVVIYYNYLVYVQEQQRGEDKN
                     RPAYLGRLERDIELTERDGRKVRLDALRGKIILASWVYTRCPRGCAGVIAKLKALHDE
                     HQGNPAIHLVSFTLDPDDTPEMMQTFARGIGIADDANWWFVNGDKDEVRRYMTQYFKF
                     RPVQDLPEGDRLTPDDKYIHDLRVVLVDAQGHVRGSLYDLMNADPEFAKYWDAQLRKD
                     LAWLLKEKPAQPADAPEPKN"
     gene            15261..15695
                     /locus_tag="PLP58_01450"
     CDS             15261..15695
                     /locus_tag="PLP58_01450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009960653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF420 domain-containing protein"
                     /protein_id="HRJ07217.1"
                     /translation="MSVYELPKLYTLFNACALLHIILGLFMIRLRQPKAHIASMVIAL
                     MFSTAFLGCYLYYHYHAGHVVFQGRGFARVFYFTILFTHIPLAVINLPMIILTVVPAV
                     RHRFDRHKRMARWTVPVWIYVSVTGVIIYLMCYVWYGPPLRS"
     gene            15819..17552
                     /locus_tag="PLP58_01455"
     CDS             15819..17552
                     /locus_tag="PLP58_01455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ07218.1"
                     /translation="MKTLLHCLFLLAPGFLLAELSVPRFFSNHMVLQRERAAAIWGKA
                     DAGAEIALSFKGKSATAKAGADGRWRARIETGAADATGAALTITAGSEKIEIKDVLVG
                     EVWFASGQSNMYYTMNRSPEYEGLIAESNHPALRMFNAPLVTAAENQDDIEGAWQAST
                     PETVPGFSAVAFFFARKLHLDLGIPVGVIKSAWGGKPVETFTSREALNTLPGTKALVE
                     VMLKDEEGYDQEKANAAYTAKLEQWQATMAAAKGKSAEERRRLPKKPDAPKRPLLTEG
                     RPGVLFASMIHPFTGYTLRGAIWYQGEGNAKAGAVPYDQTLPLMINDWRRRWGDEFSF
                     YFVQLASYRAPSTEPGQPDPWALCQDRMRRVLGTTPKTGMAVINDVGEADDIHPKNKK
                     DPGERLARWALSADYGREMVVSGPLFKEASFNESEVLISFDHCGEGLKSRDGGPLKRF
                     EVAGEDKVWHWADASVKDEFTVSVSSSKVGKPAAVRYAWASNPEGANLVNSEGLPASV
                     FRTDDWDDVEVKVDTTAADAAAARRALASEIKALAAERAKHDRQSPEYKALNRQLQER
                     MARFKKGAEKK"
     gene            17646..18137
                     /locus_tag="PLP58_01460"
     CDS             17646..18137
                     /locus_tag="PLP58_01460"
                     /inference="COORDINATES: protein motif:HMM:TIGR02989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HRJ07219.1"
                     /translation="MRLFIASEPEVLRYILAVIPNVTDARDVLQETAAALWRKMDGYD
                     PAAPFTPWACRFAANEIRAFLRREHRQRRWLDADVAELLRVHQEQALQSDDGAAEHLR
                     ECLESLPPAQHELLRRYYFEEQPVEAIARALNRTADAVYKTLQRSRQALADCLKRKQE
                     AAA"
     gene            18134..19654
                     /locus_tag="PLP58_01465"
     CDS             18134..19654
                     /locus_tag="PLP58_01465"
                     /inference="COORDINATES: protein motif:HMM:NF016648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FecR family protein"
                     /protein_id="HRJ07220.1"
                     /translation="MRELAARYLNGAATEAEVARLSELLRADQGAREEYLRLADVHAR
                     LAADTSLWTHERPRNQTVPRWKGWLLPFAAAAAVLLMAALFWPAEKAAPPPVATLLRA
                     EDAVWAGGSPNEGGRLNAGPLRLSAGRALLRLDGGAVLLLHGAVDARLESGGSVALLA
                     GEIHAQVPEAAAGFTLRAPGGDVVDLGTEFVTRVSHDSMAEVTVVKGEVEVRPRQGPA
                     QRLTTGKALAMERDGSVRATAARLPVRAWEDWEIKPAAQRREGALLVHDAFEYPPGSH
                     DPAALTGGAGWGGPWSLLTDSQGARIYSGASRTMETGAFGNAGAWLAPAGKNLRQRRL
                     AQPLDLAQDAVRYASLAWFEESLPTGRRKPSASPASGLSLTFRSLEDAAPGRVGLRVD
                     HLLRPRIETGMGQGFVSRVRANEGRRLLLVAKILSRREGEDEIMLRVFDADDPPGAWE
                     PEEWDIRTRGLRLDAVLDHVILQSSGPSPRRIEEVRLASAWLDAVTGWSAEMSNDE"
     gene            19663..22059
                     /locus_tag="PLP58_01470"
     CDS             19663..22059
                     /locus_tag="PLP58_01470"
                     /inference="COORDINATES: protein motif:HMM:NF019223.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PSD1 and planctomycete cytochrome C
                     domain-containing protein"
                     /protein_id="HRJ07221.1"
                     /translation="MNRPALFLLASATALVAEDMAFFETKIRPVLVEKCHSCHSAGAE
                     KVKGGLLLDTREGIRRGGDSGPAVTPGEPANSLLLTAIRYQDRDMEMPPKEKLPASVI
                     ADFEKWIAMGAPDPRNGSAAVMPPTVDIEEGRKHWAFQPISNPPVPQVKDESWPRTDM
                     DRFILAALEEKGLRPVADAVTEVLRRRIAFDLTGLPPDQTDPTDPSDPTDLLNSPRFG
                     ERWARHWLDVARYAESAGGGHNVLFPLAFRYRDYVIDALNADTPYDQFIREQLAGDLL
                     PARSDARRNAQLSATGFLCVGVKDLRENSNHRYRMALADEQIDATSRAFLGLTIACAK
                     CHDHKFDPIPTSDYYALAGIFTSSEPLLGARRNRQRHPLGAGLAPMAGAPVVFSDADF
                     AQMLKLRVDATYARLAVRDEKYRILIEKGLDRKNTEKHNAMLEAIPSVQKLMRVVEDT
                     EAACEALRVRYDAALPHSLMAMREDKPEDCAIHIRGEDTQLGPVVKRGFPQVLATVET
                     APVPQDQSGRLQLAEWIASPRHPLTARVMVNRIWQHLFGAGLVETPDDFGLTGQPPSN
                     PALLDHLASRFIAHGWSVKKMIAEIMGSRVYQLSTSHDAENYEADPANRLHWRMNRRR
                     LGSDALFDAVRHISGDLALERPAPVIPPTPQDDRVKSMDLKAWFAPTARHRTIYQPVL
                     RDHVPEDWKLFDFPDPELVTGRRAQTTVPTQALHLMNSPFIAEHARKTAALLLPSATS
                     TETLVRAACERILNRAPTPDEIREAAAFLKSFPANDKSESAAALCQTLFGSAEFLYLY
                     "
     gene            22063..23412
                     /locus_tag="PLP58_01475"
     CDS             22063..23412
                     /locus_tag="PLP58_01475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009958163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1501 domain-containing protein"
                     /protein_id="HRJ07222.1"
                     /translation="MNPPPFSLSRRRMLRGTLSGFGYAALAGMCARAATREPHFSPKA
                     KRVILLYMQGGVSHLDTFDHKPKLQADSGKEYATGKGGRLLGSPWKFKQHGQSGQWIS
                     ELFPHLSKRADDLCVLTAMHTEAQAHETAVPMFHTGNPLQARPSLGAWVLYGLGAETE
                     NLPGFIAMNSLRQFGGNNHGSAFLPASFQATHVKPGGKDGGGDPVANLKAAHLTPEQQ
                     QAQLRFLHRMNSRRLARDGVNHDLEALISSYEMAFKMQSTVPDLMDLGRESEATLKAY
                     GIGSTATDTFARQCLYARKFAEAGVRFIEIGTGGWDHHKDLKAEMTRSAAAVDQPIAA
                     LLADLRQRDLLRDTLVVFAGEFGRTPFAQFDNGRDHNNRAFTVWMAGGGVKAGHRHGL
                     SDDYGREGVEGRVHIHDLHATLLHLLGLDHERLTYRYSGRDFRLTDVKGEVVREVLA"
     gene            23454..24827
                     /locus_tag="PLP58_01480"
     CDS             23454..24827
                     /locus_tag="PLP58_01480"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HRJ07223.1"
                     /translation="MKPLIIIAIMLAGVLRAAPPNILLILADSQTTALGCHGAPHVET
                     PNLDKLAARGLSFTRAWCQHTIGSASRVSFLTGLRPRTLGISGNYRYAPDLPKNAATL
                     PAWLKAQGWRTSVAGRVFPRRSDYATSWDEIMLAPEDEGENASGMTTVLGVPVRSVEV
                     DGKKERQGDLLVLRSVKAGDDGTPDGRVARECVAQMEKAAAAGKPFFAAVGFMTPNFR
                     SPVPQAWLEKFKDRPAPDPLPGPQGAPECAQFGGILPERQVTEEERRQFIAGYRAQSA
                     FMDSQAGLVLDALEKSGLMENTLVVFTSANGYHLGDHGNLWGSRTLYASAAQVPLIIA
                     GPGVAAGQVCERVVEIMDVYPTLMEHLGMKSPRVMDGESFAKLLKNPALPHDDPALTS
                     VLNYGPHSGVAVRTAFASFMRWGRDTAELYNLQTDPHELRNLAGDKSMSSALRKMEAL
                     LEKEVAR"
     gene            24868..26154
                     /locus_tag="PLP58_01485"
     CDS             24868..26154
                     /locus_tag="PLP58_01485"
                     /inference="COORDINATES: protein motif:HMM:NF013866.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HRJ07224.1"
                     /translation="MHVYLPPKEKANGGAVLVCSGGGFSILAWDLEGTEVAEWLNSIG
                     FAAVVMKYRASTAHHGNNLNEQGNASLKAVGPLMDAQRAMSLTRSNAADWGLDPQRIG
                     ILGFCAGGETAGLTAILRDQRLYAKLDAADEYSCAANFALPIYPGGFYGKATGGLKPY
                     LKVTKDTPPMFFAMAQDDHVNSLNCTVLYTALSQAKVLSELHLFIRGGHGYGLRPTLT
                     PFTHWPNRAAKWLKEMGCCEASSDWAKAQTTLGNPADYLPRNVMQITAFGERAEFSHD
                     SQRVLFLSKQYGDVMEYTIRSGQIRCLTQHFKHHGFNRVMVLSNGDYLLTGPDETFDS
                     TSKEARLKARHFAKMFVLDRTLTKPPTPLGSSPMKGLPFLARNSKSLGRITSRAGIAR
                     PRSRWVILFMKTALPSLPMSRSYSPRRISPKASDQK"
     gene            26151..26579
                     /locus_tag="PLP58_01490"
     CDS             26151..26579
                     /locus_tag="PLP58_01490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ07225.1"
                     /translation="MIEAHNFTPDDKAITLTAYLIEGGHNTEGYTLDLESKKLTNFTR
                     TPDDYEEVEGIFPDGKSTLVERNHSVGKPWPMVDAWRVWFDGSKEPQRLTHFLDFKGY
                     KASNYVVSDDGRLIAFQLGISGDEAGVGYGIFLMEIKARP"
     gene            26666..27940
                     /locus_tag="PLP58_01495"
     CDS             26666..27940
                     /locus_tag="PLP58_01495"
                     /inference="COORDINATES: protein motif:HMM:NF019843.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ07226.1"
                     /translation="MKHAASAFLAILVMSLSLPVAADDHAELAALRQRAEKRDARAQL
                     GLAYRYRDGKGVAKDDVEAMKWAHMAADAGNADAMDFVGFVYLRGAVVERRPEIAFGY
                     FKAAADESAQAAFNLGQCYFGAQGTEQDCAKALEWWEKAAAMGHGRAAATAAMAYHSG
                     EGVAMDAVKARRLAERAAELNNPGGLVVLGEMQFQAGDLDAARASWTRASKLQPTSAT
                     GHPSQPSDNASAQQGADLLKLIDYRKSNSEPGKFAFVQMPHIYQGYNNCGSTACATFA
                     RFQGSEIGGWDYKKLCPSPLGTGTDWGHLLDASKKIGQKWKLITFTPDDAGFGEATAF
                     LRSELDAGRPVVVDFKYIGPQYPNGSAGHTLSVCGYIAAENLYILCNPAVATPGLQLI
                     TADDLKNFWRSDHYGALSKGVLSRPAFVIDRS"
     gene            27937..30180
                     /locus_tag="PLP58_01500"
     CDS             27937..30180
                     /locus_tag="PLP58_01500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ07227.1"
                     /translation="MKSSVVILLAAMLPSFAAAQPPIIAGIEKAGVENVTGAVLMSEL
                     ACAACHACMQTVFAPKTAPDLSAIGSRAHPAHLRQFIASPSGVKPGTTMPDVLAHLPE
                     NERNEAAEALTHFLATLGKPAPSALPTAEAIERGKGLYHSVGCVACHSPEQPLPGSVP
                     HGPLAEKYTLASLTTFLLDPLAARPGGRMPDCQLENGEAADIASYLLREQKTPLAPFQ
                     PDAALAARGQKLFAEHRCNACHTTGTKIEPPSLTALNKLRADEGCLSSKKGAWPHYPL
                     SEGQRTALRSALTDEAKSFSPAEVAQITLTRLNCISCHVRGEIGGVSTERSDYFTGRD
                     ETLGEQTRIPPALTGVGAKLKEKWLREVLVNGASARPYLHTRMPRFGAAQTAGLVPLF
                     KQLDPPLTAAFTRVEADKKPREIGRELAGTKGFNCIACHTFRGRSASPIRALDLTTMA
                     ERVEENWLHHFLAHPQKFVPLTVMPGFWPDGKSLLPAILGGDPGMQRDALWQYLAQGP
                     EAREPVGLVLEPLVVKVLNEAVIVRRAFPGIGKRGIGIGYPGGINLAFDAELMRLATL
                     WSGDFIEASSLWRGQGSGQARILGKPVVDLPPGPAFAVLATADAAWPALDMTTERSPF
                     SFKGYSLDTQQRPTLKYALPGAEVTDFFMERRDDSGSLYLERTLTFSSPPPDGFHLRV
                     ASGTMMEAQGANTFAVGPNLVIKMSARAVLRDAGDAKELLLPVRGDLKLEYHLKPAST
                     EGKGRKP"
     gene            30177..31661
                     /locus_tag="PLP58_01505"
     CDS             30177..31661
                     /locus_tag="PLP58_01505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ07228.1"
                     /translation="MKRLTQILIVCHLLIAPALLAEDLGDTWGTADREAAFYRIVNVP
                     MPEDVYVEAGSFVSLPDGRLAVGTRRGEILVFSGVSADTPKPTVSTFASGLDEVLGLA
                     WKDGAFYATHATEVTRISDTNGDGSADHYDTISDAWGFGHYHEFALGSKFDASGNLYV
                     ALGLSESYHSKALFRGWALKISPDGKTTPLCSGMRSPLGVGENESGEMFYVESQGPWN
                     GSCSLKHLKPGGFMGHPVSFNWYPHAPEMGPVPVMPNTPSRMETERKRVKELVPYAVV
                     FPYKKMGRSISAFEVCRARGKFGPFDDQIFVGDYTLSVVMRATTEKVNGVWQGACYPF
                     REGLGTGILAVHFTQDGKLITGGTNRGWPVRGMKDNLLQRLDWTGRVPFEIRDIRAQP
                     DGFTIRFTRPADAAHASKTESYQLETYTHIYQQGYGSPEVDHTRPTVTAAEVAPDAMS
                     VRLRVKGLMQGHVHDFHLPDFKSQDGETLLHDRAYYTLNEIPKP"
     gene            31747..33210
                     /locus_tag="PLP58_01510"
     CDS             31747..33210
                     /locus_tag="PLP58_01510"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arylsulfatase"
                     /protein_id="HRJ07229.1"
                     /translation="MKPIAISLFALLAFFCSHAFATPNILVILADDVGWGDAGCYGGM
                     LVKTPHIDRLAREGQRFVNGHASAAVCTPTRYSMLTGQYSWRQSGAGLNKGVSNGDSP
                     LLIPTTMTTAPALLKQAGYRTALIGKWHLGFGDTPPDYNQELRPGPLEVGFHEFFGIP
                     ATNDRLPTVFVRDHKVQGLDPADPIRLSYDKAQAESQGLTAWAAGRSRIGWGKGGKAA
                     WWKDTEISDTLTRECVQFIERNKEQRFFLLYAPHNVHAPAIPGPRFAGTSGLSPRADM
                     LQELDASIGELLQTLDRLALANDTLIIYSSDNGAYVNDEKGHKPTGPYRGKKSQLWEG
                     GHRVPFIVRWPACIQPRVSQDIASTIDVPATVCAAAGVTVPKDALPDSFDLLPAMLGE
                     KAASKRDHLILMSGNGDLAIRGDRWKYIPNLAVADGWYANKKKSASAPARPGLFDLSK
                     DPGETQNLIKEHSAEAKRLAELMAKDQASAVTRPEGK"
     gene            33297..33848
                     /locus_tag="PLP58_01515"
     CDS             33297..33848
                     /locus_tag="PLP58_01515"
                     /inference="COORDINATES: protein motif:HMM:TIGR02989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HRJ07230.1"
                     /translation="MDADHTPAAPHDPNEGFLRLWTHHEPELRTFVHACLPCAAEVDE
                     VMQEVSLVAWRKFSTLEDHGQFPRWACLIARYEILMARRRHARDRLVLDEDIVEKLAE
                     EGAEEMSERHRQLNALDDCVAKLPRERRELALAAYAPETSMKELAARLGRTEGSLYQL
                     LARIRQELLRCVEHTLAQEARMS"
     gene            33845..34810
                     /locus_tag="PLP58_01520"
     CDS             33845..34810
                     /locus_tag="PLP58_01520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ07231.1"
                     /translation="MTQEQLDLLHGYLNGTLDEAGFARLQALLRENPDARRTLRTFST
                     VDSKLYQLAAINPETVRLLAEPAGVSKISTRWLSWRPLTAAAAGLVLGLFSATCVFAY
                     SRLSPFSAAEVMAPAVTLLDEGFEAGDERFQTGFPVETGVWGGDEVQVVGERDGITPV
                     AGQRMLRVEPDEASNNSNLDRILDLSGLPMPADGGTRHVEVTAWFHADEPGLRSRYML
                     RAAVFAESAGDLRGQWVNVPWQEMESQAVALAKRGVSTTPDMEGWQKLTIRVEAPPEA
                     RSLVVSLSTRLVRMPDRRAAQYMDEVTAALWLLPPPKKTKRNLLP"
     gene            34807..36225
                     /locus_tag="PLP58_01525"
     CDS             34807..36225
                     /locus_tag="PLP58_01525"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HRJ07232.1"
                     /translation="MTARTLLTSLLFCFATSSMAANPATEVKPPNIVIIFADDWGWGD
                     LSCHGHPWLKTPHLDRLAAEGTDFQQFNVLNPVCSPSRAALMTGHYPARYAIHQHFAA
                     PQSNAARNMPDWLDAKAPTLPRFLKQAGYRTAHFGKWHLTNRHTHGAPKPDAYGYDEW
                     SVFNGGAEWPSSDGHACGRDAASFVRANAGKPFFINAWLHESHLPHVPTAASMEKWKH
                     LDPQQQVYAAVITDGDNAVGLILDALKDAGLADNTLVMFSSDNGPEHTADKPGSKEDA
                     DAGTKGYNGYYSVGSTGGLRGRKRSLFEGGVRVPFIVRWPTQTPAGIKNDTTAFTAVD
                     LLPTLCAAAAVALPESYRGDGENLLAALQGESVKRTRPIFWEWRGGGGADHDWPNLSV
                     RDGDWKLAMTHDESRVELYHLADDRAESKDIAKNHPDTVARLVKKAKEWKSTLPANPN
                     ADCISTADRSQVASPKSKRTKP"
     gene            36222..37058
                     /locus_tag="PLP58_01530"
     CDS             36222..37058
                     /locus_tag="PLP58_01530"
                     /inference="COORDINATES: protein motif:HMM:NF018038.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ThuA domain-containing protein"
                     /protein_id="HRJ07233.1"
                     /translation="MIQLLKLLAAIVLAATGIGVFAHDTATPSSPGGDRIKVLVYSDT
                     AYYRHPDIPGINRWLVLLGHENGMDVDITEHPKDLTPQVLAKYDVLLLNNANELDKII
                     PEQQRQSVEAWFGTGNKGIVGLHAALVRQTGWPWLNQLGGCDFNSDSDFTKAKVTVDA
                     AAKEHPAVKGQPAEFWIEADWTNHDRPVTGLPGFQVLLRVDESTYTPVRDYFTTRGGK
                     PMGADHPIAWTNEPAGGGRFFYTEFGHDLRSLATPFVRQHILEGIRWAAATKKSEVKS
                     AK"
     gene            37148..39211
                     /locus_tag="PLP58_01535"
     CDS             37148..39211
                     /locus_tag="PLP58_01535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009962952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HRJ07234.1"
                     /translation="MNLRGLLFLILFSCATAAGLAQNSAEVIVYDSTPAGFCAAIGAA
                     REGASVILLEPTQHIGGVNTGGLSLSDSFGCIRESLGGLWREWHERIVADYAARGVQL
                     PYQVNDVNEVKWTYEPHVAMRVTRAMLDEANVTVLTGRYLQSVKKDGARITTLVTKNG
                     SFTGKVFVDASYEGDLMAAAGVSWTIGREGRDEFGESQAGVTHGIKKPRVKVSGFDET
                     GNPLPFITGTKLPPDGTPDDMLQVFSFRLVLTRDPQNRVPMPAPASYDPARWELARRF
                     MAVHPDKVGIDLYELPNGKFDGNDGIHKLLSLGMVGGAQKWCGADEAGRRAIWEEHKQ
                     YTLEFYHFLSTDPSVPKKARDTYAALGLCKDEFAEHGHFPPELYVRQGRRMEGEYFMT
                     QNDMDQSPSKEDPVGVSSFPADSHDVTRIALPAGGVITEGAFLRWVQGKRHGYPHQVP
                     YRALIPKRDECANLIVPVAFSCTHVVICSARVEPTWMVLGQSAGVAAAMAAKRGASVQ
                     DMPYADLKPRLEAQGQRLVLPDQSKTPPQTATGLIPVTSLPGIVLDDKDAELTGDWAH
                     STRFKPCIGSGYVISGAKDARGDGKASATFRFKAPKSGEYQVLMAYSAHATRASNVPV
                     LITSGGRETKLTVDQTRPLPAGQNFQRIGTAALNAGVETIITIKNTDTTGFVILDAVQ
                     LVPVK"
     gene            39231..41375
                     /locus_tag="PLP58_01540"
     CDS             39231..41375
                     /locus_tag="PLP58_01540"
                     /inference="COORDINATES: protein motif:HMM:NF019223.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1553 domain-containing protein"
                     /protein_id="HRJ07235.1"
                     /translation="MKTILHSLAISLLFVLAVRAEQPVDVYRTWTDTQGRKVDATFRG
                     FEDGQVFLQTKDGQLYRFEIGRLGAEDQKAALSMKPTGLGIPADPAIAMAAAKVDALV
                     DAGLRSKNQRPNPLASDEQFVRRVYLDIVGRIPTRDEALELIEDQSVSKRAKLIDRLL
                     AGDGANSHLYNYFADMLRIRDIAGKLGVFYTYQEWLKGELKKNTGWDKIVHQMLTAEG
                     KLLENGATGYLLRDDNMPLDSLSLTLTSFLGADVGCAQCHDHPFADWTQRQFYEMAAF
                     FGATDTNGGKGKDRPGTKELREAMAVIAEESRQRKEKNPDAGRDKSVKLVIYLERING
                     FSVYDDDVNELKLPHDYKYKDGKPGDLVTPKLLTWSKEDARLAAYREFTATGGREPEQ
                     LRAQFAKWLTAPDNPRFAMTIANRMWKRAFGLGVREPVSDLDDPAAASNPPLLQHLAR
                     EMVRVKFDLKAFMRILYNTQTYQREATSHDIDLGQPYLFPGPLLRRMTAEQAWDSCAT
                     LAVGTAVDQFHSERAEKYVSIKNINTNASPAEILEQVRNAIARNADAPNVSKEGKGAK
                     IAMQTAKNLTSTDEDGVSITPPMLGEMVIARASELLQPEREYHFLRQFGQSDRQLSDS
                     NSDEGHIPQVLMLMNGAAQDVIGSTRSLAVQKAQEQAAPEARIESLYVSFFCRKPTAR
                     EVTIAKELLSSDLTLSDLAWVLFNTREFIFVE"
     gene            41423..42703
                     /locus_tag="PLP58_01545"
     CDS             41423..42703
                     /locus_tag="PLP58_01545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009960473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1501 domain-containing protein"
                     /protein_id="HRJ07236.1"
                     /translation="MNTRILDPESRRVFIERWAKATLGVTVLHGTAGQMLAAESKTAV
                     PLPSPGKGKNLIFLNLIGGMSHIDTLDPKEGPTQGPGKPIKTKAGFQLGEIMSKLAGQ
                     ADKISLIRSMTSKLGTHSSAQYFIRTGYEQRTTIMHPTLGAWAQHFKGPSHPSLPSSV
                     CVNRNPNNANGFFPASHSPLPVLDPDVGLQNSQPAVEAGRFTKRMAFLDRLDGEFRHR
                     FQTASVKSYTDFYKQTVALMGSPDLKAFSIAGEPAAVREKYGKTRFGSGCLLARHLIE
                     SGVRCVEVASDGWDMHGNIAEGLHSHGAEMDQAVAALLDDLGQRGLLESTIVALCTEF
                     GRGPVVNVNGGRDHYPKVFSTMLAGGSIKRGYVHGASDKGGYEVADQPVTVQDLLSTI
                     GHAMGLPVDEVTMSPSNRPFTIGDKGKVITDLFA"
     gene            43156..44730
                     /locus_tag="PLP58_01550"
     CDS             43156..44730
                     /locus_tag="PLP58_01550"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arylsulfatase"
                     /protein_id="HRJ07237.1"
                     /translation="MRHTIALLATALLLCAQAPAATADKPNILIIYADDLGYGDVQCN
                     NPERGKIPTPNIDKLASQGMRFTDGHSSSGVCSPSRYTLLTGRYHWRTRLQRGIVGVL
                     GEPLIAPDRMTLGTLAKQQGYRTACIGKWHLGWNWPVTKEQRPMLLPDKKTAVAATEQ
                     QVALWRDIFSKPIAGGPTTRGFDLYFGTDVPNHPPFCFIENDQTVGIPSEFLSAALLV
                     NNQASEQGPALKDWKLEPILPALGERSAQFISESAKEQQPFLLYLPLTSPHTPISVNA
                     EWKGRSGLNPFADFVMETDAVVGRVLDALEKSGAAENTLVIFTADNGCAPYIGVKDLE
                     AKGHYPSGPLRGYKADAWEGGHRVPFIVRWPGTVKQGSVCHQLVYQADILRTLAELWS
                     VTLPDNAGEDSFNLMPLLKGEDKPIRKNAVSASKDGVPAVRSGPWKYIPAPGSGGWGS
                     GGDQSQPVQLYNLADDLGETKNLAAAMPEKVAEMKALLEKLITDGRSTPGAQQKNDVE
                     VVRYPRENTPKKSKKG"
     gene            44747..46264
                     /locus_tag="PLP58_01555"
     CDS             44747..46264
                     /locus_tag="PLP58_01555"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arylsulfatase"
                     /protein_id="HRJ07238.1"
                     /translation="MKSFLLLLSSFVFCQTSFSALPNILLILADDLGYGDVRCYNADS
                     KVPTPNIDKLASEGMRFTDAHSPATVCTPSRYSLMTGQMAFRVPNGGTVFTGAGGPSL
                     IFKGRLTLPAMLKKQGYTTAAFGKWHVGLTFRDAEGKPIHEGGLEAIRRIDFSRRIEG
                     GPLDHGFDRFFGTACCPTTDWLYAFIDGDRIPTPPTSPLDRSTLPKHPYANDCRGGLI
                     APDFDMQEVDMLFLQKSRDFIAQHAHNSPKQPFFLYHATQAVHLPSFAGKDFRGKTHA
                     GPHGDFIAEFDHIVGELMRELEKHGLAQNTLVLLSSDNGPETTSVVHMRADHSHDGAK
                     PWRGVKRDAWEGGHRVPFIVRWPGKVKAGTTSDQTLCLTDILATVAAITGTELPHDAA
                     EDSFNLLPALEGTATAPIRPYLLTQAFAGQRTLSIRRGQWKYIAHRSSGGNNYDKGEM
                     KPFALPDTDPETPAQLYDLATDPGETTNLYSKQPEIVKELQALLEQSKTTGRSRP"
     gene            46381..47439
                     /locus_tag="PLP58_01560"
     CDS             46381..47439
                     /locus_tag="PLP58_01560"
                     /inference="COORDINATES: protein motif:HMM:NF014691.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HRJ07239.1"
                     /translation="MLPPVFTITPEMLRLIAELEDFKGQWRMIRSLSPEKLTSLRKVA
                     TIESVGSSTRIEGSRLTDRQVEDLLGRLHITEFKTRDEQEVAGYAYVIDQVFDAFDQI
                     PVTENMIGLLHRDLLRYSTKDERHRGHYKTLPNHVAAFDADGKEIGIVFQTTAPFDTP
                     REMEALIAWFIKADRERSLHPLLLIGSFIVVFLAIHPFQDGNGRLSRVLTTLMLLRAG
                     YAYVPYSSLESVIEASKEGYYRALRRTQTTLQAKKPDWEAWLMFFLRSLVKQKDALAK
                     KIAIEDTHATDLHPLAKNILTHFADHETLTLSHLVKLTKGKPSTLKLRLKELVQHGHL
                     LPKGQGRGAHYVKNTASL"
     gene            47466..47741
                     /locus_tag="PLP58_01565"
     CDS             47466..47741
                     /locus_tag="PLP58_01565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ07240.1"
                     /translation="MPKFYREAILATHSIRGTTPATEVGQLETWPKVARALDELDSSG
                     RCHAIYSDWLLQLDEPGGDVQIQIRDLLLGQGALAANYFVTVYLVAC"
     gene            complement(48369..>48787)
                     /locus_tag="PLP58_01570"
     CDS             complement(48369..>48787)
                     /locus_tag="PLP58_01570"
                     /inference="COORDINATES: protein motif:HMM:TIGR03696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="RHS repeat-associated core domain-containing
                     protein"
                     /protein_id="HRJ07241.1"
                     /translation="LETMTWLSRDPAGFVDGPNLYAYVKQNPWSGFDPKGLNVKATTK
                     KETNKQGNQYDHTKIEMNVVVVDGSKGKTVGKDKGNPIKLNLPKNLMGYKGGSELHPM
                     QIEAIQASYDSNLLNLKPSGLYPWGQTNGPKYKIHN"
BASE COUNT         9305 a        15924 c        14218 g         9340 t
ORIGIN      
        1 gcgccgcggc cagcgccgcc tcgtcaacca gcgggccgcg gccggtgttg atgagaaagg
       61 aggagggctt catcagaccg agtgtgccct cattgacgag gtgcttcgtg gcatccgtca
      121 gcgggcagtg cagagtgacg gcgtcgcttt ggcggaaaat ttcatcggtc gtggctggtt
      181 gaacaccctc cggcggcggc gttttccact cgcgccttga tgccagcact ttcatgccaa
      241 aggccagcgc gatgcgcgcc accgcgccgc caatatcgcc atagccgatg acacccaggg
      301 tgcgtccgcc caactcgatg acagggtggt cccaatagca gaaatccggg cacgcggacc
      361 atcggccgtc gcgcactgtc cgcgcgtgat gcccggcgtg gttggtcagc tccaacagca
      421 gggcgaaaac gagctgcgcc accgccgggg tgctgtagcc gggcacgttg gtgaccacga
      481 tgccgcgctc ctttgccgcc gccgtgtcca cgatgttata gccggtggca gtgaccccga
      541 tgtacttcag gccgggcagc gcctccatca tcgcacgcgt cacgggcgct ttgttggtga
      601 tgaccgccgc ggctcccgcg cagcgcgcta cggtttcgtc aacgggtgtg cgtggatgaa
      661 tctcgcaggt gccgagggcg tcgaggggtg cccaggaaac atctcccgga ttggcggtgt
      721 gggcgtcgag caggacgatt ttcatgaggg cgaccctttc gtcggagctg tgacatcgcg
      781 caagcggcga agacaagtct gccaaagaga cgcgcagacc ggtgtttgcg gattttgaaa
      841 caggaccacg aaggtctggc atcgagaact atcccaggtt gtgaacaatc agaccgactg
      901 acagcgaaaa cccgcatgcg aactgaagga gaatctgttt ttcgggcgat ttcatgccac
      961 tggaattcat gaaatccgca ggcttgcgct ttgatacagg gagggcagtc tgccttgcgc
     1021 tcaagttttc cacaaaaaac gaattcgccc ttgcattgtc ccggcgagtt tctaatcttg
     1081 gcggccttgt gaaatttttc acaagctcga aacgggtggc cgcccgtcgt tctcaatcat
     1141 gaaatatttc tttctcagct acctgtttgt cgccgcgatt gtggtggcgg cttttggcac
     1201 tcgcggcagc aagtcagaac tcccgccgat cgagattctg ccggacatgg actatcagca
     1261 gaaagtgaag tatcaggcaa aaagtgactt cttcgccgac ggtctcggtg cccgccgtcc
     1321 ggtggctggc acggtgccga tgggcttcga agtgccggca aagcccgctt cctccccgga
     1381 ataccagcct ccggccttcg gtttcagtca cggcacgggc tattacaaca ctggcaaatt
     1441 cggcgattac tacggcgacg gtctgccgaa ggagatcgag ctttccaaat cccttctgga
     1501 gcgcggcgag cgccagtaca acatctactg cgcggtatgc catgcggctt ccggcaatgg
     1561 caagggtgtc acctccaaat acggcatcct gaccgccttc aacttccaac agcccggcag
     1621 cctcgatccc gccaacgccg ccgcttaccg cgcggatggc gcgatgtttg atgtcatctc
     1681 caatggcaaa ggcttgatgg ggccctatgg cggctcgatc tccgttcaag accgctgggc
     1741 catcgtggcc tacatccgcg ccatgcaggt ggcagtgaag gaaaacaacg tgtccgtcca
     1801 gtaatcgcag gcaattccag gcatgagtca ccacgtcaca ttcaaagatc tgcccgaggg
     1861 cggcgaaaaa ttcgacacgg ccaaaggggc caagatgatc aacgcttttg gcgcggcggc
     1921 ggccatcggg cttgccggat cgggttattt cttcttctca agaaccggtg agtttgccta
     1981 cagctggctt ttcgcgttct tcttcgcctt cacagtggtt gccggagcgt gtttttggat
     2041 tcttctgcac agcgcctcga actccggctg gggtgtggcc atccgcaggc tgtgggagca
     2101 gctcgccaac atgattctgc ccctggcggt tctcgggctg ccactgctgc ttgttccgac
     2161 ggtgcaggac tcgctctatg agtggatgga gcatcatcgc gaggcggctc atcacgcgca
     2221 tgaaaaaggc gagtcagtgg ccgatgctct gcaccacatg gcggaaggca attcgcatct
     2281 ccacatcctg gtggacaaat acggctactt gaacatgtgg ttctggaagt tccgcttcgt
     2341 gttttacttc ttcgccctct ggcacatcgc cagcaccctg aggaggaaat ccgtgctaca
     2401 agacgaggat ggcgacatca agcacaccgt caaatcccgc accttttcct gccgctggct
     2461 gctgttttac gcattgacgg tgactttcgc ggcggtggac tggctgatgg ggctcgacta
     2521 cgcctggttc tccaccatgt ggggcgtgta catcttcgcc ggctgcgcct ggtcttcgat
     2581 ggcggtcacc atcttgctcg tcaactggct caaaagcatt gggcatctca agattgtcac
     2641 cgaggagcat taccatctca tgggcaagct gctcttcgcc ttcacggtgt tttgggccta
     2701 catcgccttc agccagtatt ttctcatctg gtatgccaac atcaccgagg agacgcgctt
     2761 ctatctcatt cgcaataccg agggctggcg ctgggtctcc attttcattg tcgtcgggca
     2821 cttcatcgcc cccttcgttc tgctgctctc ccagccgcgc aaaaagaacc cggccctcat
     2881 cggcctgatc tgtttgtggg tcgtcttcat gcatctggtg gacatttact ggaacatcat
     2941 gcctgagcgc gggccgtccc ttgccattgg cctgatggcg cccggagcct gggttggcga
     3001 tgtcttcgca ttgcttgccg tggttggaac ccttggtttt cttttcctgc gcagccttgg
     3061 caaacacagt ctctacccct gccgcgaccc gcgcctgctg gagtccgcca acgtcgtcaa
     3121 ctgatccctc atgtccccgt ccgatacttc cccgaaagct ggcgccacct tccagtgggt
     3181 gctaggcgcc ttcgcgagct ttgccgttct tttctgggtc atccagacat tcttcgtgac
     3241 tcccggtgat gccgacccgc gcgccgccga gcgtctcgcc aacagcgccg agatcagcgc
     3301 gctgcaaaaa gacgtgcttg ccaaaatggg attgagcgat cccgcgaaga aagcggcggt
     3361 ctttgacaag actctccagg tcctgaaaac ccgccctgag tccgcaagcg gccaggtcgt
     3421 gccaggctct cccacccagc tcaagcaggc cgccgaggca ccgccggcag cgggcgcggc
     3481 tccggccccc tccgccacgc cgcagaactg atattttcga tcccggaacc tatgcagacc
     3541 accccagcaa ccacggacac ccaggccgac gatctgcgcc gcgcggcgat tgacaagtcc
     3601 gtcaaaggcc ccgtgctttt cctgttcctc aatggagcct tctggctgat ggcctccacc
     3661 attcttggag tgctggccgc catcaaacaa ttcgcccccg aatttctggg cgcgtgcagc
     3721 gtgctgcaat acgggcgcct tcagccgacc catgtcaacg cgctcgtcta tggctggggc
     3781 gtgcaggccg ccctcggcgc catgctctgg atcatggccc ggcgcaccgg ccaggagttg
     3841 aaatccggca aaagcatcct gttcaccgcc gccattttct ggaacatcgc cgtcaccctc
     3901 ggtttgctgg gcattctcct gggccaaagc acttccatgg aatggcttga gatgcccgct
     3961 tttgtctggc cggtcatgct ggcgagcttc ctggcctttg cctggccgct ggcgcgcatg
     4021 tttgggcgtg ccttccgccc ggatggattc atggtcagca cctggtatgt tttgggcgcg
     4081 tgcttctggt tcccctgggt tttcatcaca gccaacgtcg ccctgcactg cctgcccgga
     4141 ctcggagcca tcggcgcggg cgtcaacgcc tggtatgtgc atgccttgat ccttctcttt
     4201 ttcgcgccgg tcgccattgg cgccgcttat tatttcatcc ctaaaatcac cggccagccc
     4261 atcgccagcg cgcagcttgc gaaacttggt ttttggcttc tggccgtcct cggcggatgg
     4321 acgggcatgc agcatttcat gggcggcccg ctgcccgcct ggatggtggc cgttggcgcg
     4381 gctgccgctg ttctcctctt gatcccggtc ggtgtggtcg gcttgaacca tcacctcacc
     4441 acgctcggca aacacagcct cgtcgccagc agccccactt tgcgtttcgt tttcttcggc
     4501 tcgctgtttt atccggtcag cggagtcatc ctggcactga tttcgaactt caacaccggc
     4561 gccaccctcc agttcaccca ggcctggtat ggcttccaaa tggtcgccgt ctatggcttc
     4621 ttcagcatga cgcttttcgg cgccatctac tacatcgtgc cgcgtctctc cggttgtgaa
     4681 tggctctccg tgcgcctcat tcgcaatcat ttctggttct ccgtctatgg cactgccacc
     4741 attgtcgtga ccagcctcgt tgccggagtc cagcagggtg ccgacctcaa ccatcctgaa
     4801 atgtgggacc aggatttcag ccagctcgct ttgaatctgc gcccttacat cgtcgcccgc
     4861 gcgcttgcct ggggactcat cacctggtcc aacttctggt tcctcatcca tctcatgctc
     4921 atggtcgccg gccttggccg ccgcagctcc gcgccgacgc tgcttgcgca tgatcacgaa
     4981 gacgaagccc ttcccaacgc cgcccacgca tgacccagtt tcgcacgttc atcctcgcct
     5041 tgaccgccag tttcggcctc ccctggctgt gccttatcgt cgtgccagcg gtcaaatacc
     5101 aggctctttc accgctgccc tatgacaagg aggctgacgg gatggacggc tactatccac
     5161 ccgcgcccat caaccgccag ggccagctcg tctatgcccg cgaaggctgc gcccagtgcc
     5221 atacacagat gatccgccca ccgcagatcg ccctcgacgg ctggcgcaaa ggctggggcc
     5281 aggatcagga gggacgcccg cccgagccgg tgcgctccaa tgagctgcgt gactaccacg
     5341 gagaaaaatt cgccttcctc ggcatccagc gcagcggccc cgacctcgcc aactacggct
     5401 ggcgcgtggc ggaccggaac gccgtccacc agaagctctt cgccccgcgc tccatccatg
     5461 actggtccac catgcctccg ctgcgccatc tttacacggt gcggctcgcc cagggccagc
     5521 cttcggagaa agccctcaaa ctcaccggca aacacgcgcc caagcccggc tatgaagtcg
     5581 tacccacaga agatgccgag gcgctcgtgg actatcttct ttctctgaaa aaggattatc
     5641 ccatcccagg cagggccgcc gccatggccg ccgccgccaa gtaacccgga gcttccgcga
     5701 ttttcatcac cgcccatgag cgcccccaac tctaccgtga acaccgacag caccgatctc
     5761 gaccgcctcc acgccgccgt ccgccgcgaa aaggccgacc tcgagccggg ccgcgaaccg
     5821 gtgccgatgt ggatcatgtt tgttttcatg ctgatcgcca tcatcgctgg cgggcagctc
     5881 ggccaaatga caggcggctg gagcttcgat gtcagcaatc cctggtcttt ggccttgcag
     5941 ggtgatcccc gtccgggcgg tgacggcggc gctggcgcgc ttgatccctt ccagaccgcg
     6001 atgaaaaagg gcgccagcgg ctacgccgtc tgcggcggct gccaccaggg cagcggcatg
     6061 ggcatcccgg gccagtatcc gcctttggcg aaatccgaat gggtcaccgg cggcaccgag
     6121 cgcctcatcc gtgtggtgca gcacgggctt gtcggacaga tcaacgttgc cgggcaaaac
     6181 tataatttcc ccggcgggat gcagcccttc ggcgcggcca tgtcagccgg cgaccttgcc
     6241 aatgtcctga cttacatccg caattcctgg ggcaaccagg gcaccatgat caccaaggaa
     6301 atggtggaaa aagtgcgggc tgacgaatcc agccgcgccg cccaatggac ccaggcggat
     6361 ctggagaaat ttgcggaagc caatgttccc ggtgaaatcc ccgctggtcc cggtgcgacc
     6421 gccgcagcgc ccgccgaggc gaagtgagac cgttgaattc cagtccattc atgaaacaag
     6481 catggcagtc atcctccgac agggcgtctt tgacgctgct ggcggcttgg ctgctctccg
     6541 ccgctgtcag ttgcggcaag gcgcctgaag ccggttcttc ctaccagccg ccgcccgctg
     6601 tcactgtcgc ggaaactccc tccatcacag gcagggtggt tttgaccggc gggctgcctt
     6661 cccgtgccgg aaaaaaattg gatgtggcag gcaacccctt ttgcaccggg catggtgacc
     6721 tcatggatcc ggcgtggaaa gtttcacccg aaggcggctt ggcggatgtc gtcatcacgg
     6781 tcaaatcagg tcagcgcgcc gccaatgttc cggactttgc cggactggtg gatcagaaga
     6841 actgcgaatt tgcgcctcat gtcaccgtca tccaggcagg ccagggactg cgcctgcgca
     6901 acagcgacct cactttccac aacatccgca tcgcccgcca tgaacttggc actcgcgatc
     6961 gcggtgtgaa tctcgacaac ctcgcccagg ccgcgcgggg cgatgagaac agcaaggttt
     7021 ttcacgaacc cggcatctat cgcctggagt gcgatgtgca tcgctggatg cgcgcctggg
     7081 ttttggtgca tgcgggcgcg cataccgcag tcacggatgc aagtggtgat ttcactgtca
     7141 cgcgagcatt gccggatggc gaccatgttg tcgaagcctg gcatcccatg tttcctgaaa
     7201 agctgaccag cacagtcacc atccgtgacg gtcgcgccga agcgagtttc aactttgatc
     7261 tcgcgcagtc attcgaaccg ccggcggacc gctgacgtaa cctccacgtt tcttaaccac
     7321 ccatcatgag cgcagccgcc accacccacg atcactcgca cggagccgat cacgcgcacg
     7381 accaccacga gccgggcttc atcggcaaat acattttctc cagcgaccac aaggtcatcg
     7441 gcatccagta tgcgatgagc ggacttttat tcctcctctt tggctttttt ctcatgctct
     7501 tgatgcgctg gagcatcgct tttccgaacg aaccaattcc aggcttcgct tttgtgagga
     7561 gcctgccatt tatcggtgat tttttgtttc ataatatgat cggacgctgg gctccgaacg
     7621 gtgtggtgaa tggtgagctt tacaacatgc tcggcgccat gcacgggacg atcatggtct
     7681 tcctcggcat cgtgccgctt ggctttgcgg ggtttgtgaa ttttgtcatg ccactgcaga
     7741 tcggcacgat tgacatggct ttcccgcgtt tgaacatgtg gagcttctgg ctctttttta
     7801 tcagcggcgt gatgatgctt gccagcttct ttgtcggcac gggctcggtg cagacgggct
     7861 ggacgatgta ctccccgctc gcctccacaa cgacgctggc agtgaccaac atctggatgc
     7921 acggccacac ttggtggctc ctggcgatgg tgttcaacat ctccgcctcc ctgctcggct
     7981 cggtgaacgt catcaccacc gtcatcaacc tgcgcgccag gggcatgtcc tggatgcgca
     8041 tgcccttctt ctgctgggcc atgtttgtca ccgccttcct gctgcttctg gccttcccgc
     8101 ctctcgaagt ggccgcgctg ctccagcttg tggaccgtgt cttcggggcc agcttcttcc
     8161 tgcccaccgg tctcatcgag ggcggccagc atctcgacat cgccggcggt ggtagtccgc
     8221 tgctttacca gcacctcttc tggttccttg cgcatcccga ggtttatgtt ttgatcctgc
     8281 ccgccttcgc catcctgacg gaagtcatcc cggccaacac ccgccgtccg ctttggggct
     8341 acaaatccat ggtttatgcc gcgctgaccc tcggcttcct cagcttcctg gtctgggcgc
     8401 atcacatgta tctgaccggc atggggacca tgatgtccac ctacttccag atcaccacgg
     8461 tgctcatctc cgtgccttcg gtgatccttt tgaccggtct gatgatctcg ctctggggcg
     8521 gctccattcg tttcaccccc cagatgctgt ttgcctccgc tttcctgccc atgttcggca
     8581 ttggcggcct gaccggcctg ccgctggcct tcatcttcat tgatctggca ctgcacgaca
     8641 cttactacgt catcggccat ttccattatg tcgtggctcc gggcaccatt ttcgccctct
     8701 ttgccggtgt gtatcactgg ttccccaagg ccaccggacg catcatgagt gacaagctgg
     8761 gcaagctcca cttctggccc tcgcttctcg gcatgaatct ggtttttgca ccgatgttca
     8821 tccagggcat gggcggcttc caccgccgct ggtatgacgg cggcgccaac ttcaagcaga
     8881 catcggaggt ttccaatttc tggaccgaat tgaactcgaa cctcttcggt ttcttcggca
     8941 tggaagttcc gaacaacctg ctgggcttga atgtgctgat gtccgtcggg gctttcattc
     9001 tggctcttgg ccaggtgccc ttcattctga acgtcttcct cagcatcaag ggcggcaaaa
     9061 aagtcgccag cgacaacccc tggaactgca ccaccctcga gtgggccacg cccacgccgc
     9121 ctccgcatgg caacttcacc tttgaacccg tcgccgtgcg cgggccttat gaatacagcg
     9181 ctcccggaat tgaccaggat ttcttcccgc aatgggagag cgagcccggc aaggccgcca
     9241 ccgcgccggc atcgtcatcc gcggccgtcg cgcatcattg aagcaccgaa aactgagcac
     9301 tccgccactt ttccctcatg gacatccctt acaccgtatc cgcccgcccc gacacgggcc
     9361 tctacaatgc caaagttggc atctggcttt tcctcgcctc ggaagtcatg ctgttcggcg
     9421 gtcttttctc ctcctacatt ttcctgcgag tcggcgccga ctaccactgg ccggtgcatg
     9481 agttgaatgt cttcctgggc tttgtgaaca cgcttgtgct catcgcgtcc tcggtcacgg
     9541 ttgtgatggc ttgggcttcc ttgaaactgc gacagatcgg caagttcaag ctttacatgg
     9601 ccatcacagt gctctgcgcc gccatcttca tggtcaacaa ggcttttgaa tacaaggcga
     9661 agttcgagca ttacgccgtc aagctcacgg acggcacctt cctgaccgga catctgccgc
     9721 agggatacga gatcaaattc ggtgatgtca gcgaggtttc cgtgctcatc gcgggtgaaa
     9781 acaaggcggt ggacggcgag cctctaaagc acttcgtccc ccgtgttgaa ggcgatcttc
     9841 cagccttcaa aatgacggat ggctcctcta tcacgctgga tcaaagcgga ttcaccactt
     9901 tgaaagaggc aactctcaaa gccgcgcgcg aaaaactgga ggaagaccgc aaaaaacgcc
     9961 gcgaggaagg cggggcgttc attgaggccg gcaacattgc cgccggcaag gccaaactcg
    10021 acgaggcggc gcgcatgaac atcgtgccgg acaccacgat caagctcaag ccggagcagc
    10081 ctgtcaccgt gaaggtgaaa ccctctcaaa ttttcagtta cacggcgaac tccctgacct
    10141 tcaaggacgg caccacactc accggcaagc tgttggatga cagcatggtg ctcgaagtgg
    10201 acggcgtgga cacccgttcc gtgccagatg ctgagaagtc cctggcctgg aaatccggcc
    10261 tcctcggcga gccttggaaa aaagcgttca ttgccaatcg cgaccaccat ctcaacgcct
    10321 tcgaggaaaa gcacggcgac cgccgcgacc cgaagaaaag cgccaccctg caaaaagagg
    10381 ctttctttct gaaaatcaaa tccgccacac cggatgcccg ggatgatgct catggcaaca
    10441 gcaagagcgc cgccgccagc gcgcccgcag acagccatgc ggatacgggg catcacggcc
    10501 accaccccgt ggttcatgtg gaccgcaagg acatcgcctt ctactcgaac tacacgccga
    10561 agctgaacac ctactatgcc atctatttca cgctgaccgg cctgcacggg ctgcacgtcg
    10621 tggcgggtgc cattgtgctc ttctatttcc ttgttttcga tggcaggcgc ctgcgcgaaa
    10681 atcccgagca cctggccaac cgcgtcgagg tgggcggctt gttctggcac tttgtggacc
    10741 tcgtctggat cttcctcttc ccgcttcttt acctcctgta accgctgaca tccatggctg
    10801 actcacctga agaaatccaa aaatccatcc gcaagtatct ctggatcggc ggcatcctga
    10861 tctttttcac cgtcgtcacc gtcggcttgt cgtatgtgga actgcccacg cacggcttga
    10921 acatcctggt tggcatgatt gtggccacct tcaaagccgc gctggtggcc ctgattttca
    10981 tgcacttgaa ccatgagcgc atcctcatct acaaggtgct ggccttcacc acggtattct
    11041 gcctcgcgct ctttctgttg ttcattttca gcgccggcga cccgctcgtg ttcgagggct
    11101 tctaaacctg caagcactcc taaagtcatg tccctcaagc acttccacat ggtcttcatc
    11161 ttcttcgcca ttctgtgcga tttgggcttt tttgtgtgga cgcgcctgct gcccgaaaag
    11221 gccgcgcagc ttggcgtgga ggagttggga atgctggcgg gctggctgag cctcgccctc
    11281 accggctacg gcgtctggta tgtggtcaaa aaaagccgcc gcatcatcat ctgacctgtt
    11341 ttttatccga accttcccgc catgaacttt ctcgcctgcg ccacctgccg ccctgatcct
    11401 ggaagcctta ttgcccaggc tcaggacatg gcagtgcttg tcatgctggg ctttctcatg
    11461 ctcggcatgg gcgccgttgg tttgatcgtc atgaacttcg cgcgcaagca gcgccggaac
    11521 cccaccctcg aagcctgaac tttccattta cccacacatg agcgtctccg acatcaacca
    11581 ttggtttggc atcacacaga acgcctccga gcacggcggc ctcgtggacc acatgctcgg
    11641 ctttgtgcat tggttcatgc tgttcctgtt catcgggtgg tccattttcc ttgttatcgc
    11701 cttcacccgc ttcaggcagt cgaaaaatcc gaaggctgat tatcatggtg tgcgaggcca
    11761 tgcctccact cacatcgaga tcggcgtggt cattgtcgag gccatcctgc tgctcggttt
    11821 cgccttcccg ttatggtcac gccaggcgga tgacttcccg acatcgccgg acaccatcaa
    11881 aatgcgcgcc ttgggtgaga aattcctttg gaacttccat tacgccggaa atgacatgat
    11941 gctgggttca tgggatctgc gcctggtgtc ctccgccaat caggtcgggc gtgaactgca
    12001 ggatccaaat ggcaaggatg acttcgtcaa ccccggcacc atgcgtctgc cggtcaaccg
    12061 gcccgtgatt gtggatgtgg ccagcaagga cgtgatccac aatctcgcca tcgttcccat
    12121 gcgcgccgcg caggatgcct gccccggagt gaagtcgcac atgtggttca agcctgtgcg
    12181 caccggcacc tgggacatca tctgcggcca gctttgcggt ccaggccacg cccagatgcg
    12241 cgccgtgctg gaggtgctcg aaggaccgga gtttgacgaa tgggccaagg agcagtccgc
    12301 cagcgccgcc gccaaaagcg cgtcgcccgt ggccgccgcg cagtgagccg gcgtcctcca
    12361 agtttctttg gcgcccggtt gatccgctca acttggcgct ttctttcttg acgccatcgt
    12421 tgagttccat gcgcgcgttc cacatccagt cccttttcac cctggcggtc ggcgtgctgc
    12481 tcgtctggtg gggggcggcg gtgaccactg aggatgtggg cctggcggtg ccggactggc
    12541 cgctgtgttt tgggcggctc aacccggagg gctggtacaa agtgccggcc ctgctgctgg
    12601 agcacgggca ccgctggatc gccactttca tcggctttca ggtgctggcc atgtatttct
    12661 ggcagttcgc caaatgccag ccccggttca ttgaggcggc gggcatcatc attacgggag
    12721 tggcgtactt gttcctcgtc ttccggcagg cgctcgcgcc cgcgggagtg atcttgtttc
    12781 tcggcctggt gtggctggtg ctgaactgga tcgggcagcg ctggacactg ctgcgcgggc
    12841 tgaccacggc ggctttgttt ctggtcattt tccaggcatc tctcggcggt ctgcgcgtgc
    12901 tgaagatgtc cgatccgtat ggcatttccc acggcaccac aggtcagttg tttttctgcc
    12961 tgctcgtgct cgtcgccctg gcatcctccc gcgtgtggtg ttcaggcggg ttgcgcatgg
    13021 ggtggcatga cagaaaaaag gcgagactcc tgggatcgct gctcttcggc gcggtctcca
    13081 tgcagcttgt catcggcgcc atcctgcgcc acacccagcg cgcgcacttg gcggccaatg
    13141 acattctcac caccaaggga atgctgctgc cgccttttga cccggcggat gtctttgtgc
    13201 tcttcctgca caaatactgg ggcttttgcg tggctggcat ggtgctgttc gtggcctggc
    13261 cggcccggcg gtggttttcc gcgatcccag gtctgcgcgt ggtgccgcgg ttactgttga
    13321 tcatgccttt ggttcaggtg gcgctggggg ttgcggtcat ctggacgggc aaaagcttct
    13381 ggtacaccaa ctttcatgtg ctcaacggtc tgggcttgct cgtttgcgct ttcctgatga
    13441 tggccggagc ctggggtgcc cgcttgatcc cggaaaagga aacgcctgct ggcagcctgg
    13501 aggctgcggc atagagtgag atcatgagcc agcctgacgc gccgccaacc ccgactgccg
    13561 ccgccgccag cggggcaggg gaaccaccgc gagggcgcgg catgatgaat gacctgctcg
    13621 tgctcaccaa gttccgcctt agcgcgctcg tgatcgtcac gactttcgtc ggcttctggc
    13681 tgcggcgcgg gccggactgg agcggctggc tgctttttca cacgctgctg ggcagcagct
    13741 tcgccgcttt tggcgcggcc gtgttcaacc agctcatgga gatcggcccc gactcgcgca
    13801 tgacccgcac ggccgaccgt ccgctgccct ccggtcgcat cagtccgggc gcggccttcg
    13861 gcatcggctg gctgctcagc gccttcgccc tcatccatct cggcgccaag gcgaacatcg
    13921 aggccgccgc gctcaccgcc ctgacgctgg ccgtctatct tttcatctac actcctctga
    13981 aaaaacagtc cgccgccaac acgcttgtcg gcgctgtctc cggcgcgctg cctccgctca
    14041 tcggctgggc gggcgcggcg ggtcagacgc ctgcggacgc gcgctacttc cgctgggagc
    14101 tgctcctcga gccgggcgcg ctttatctct tcgcgctgct gttcctctgg cagctcccgc
    14161 atttcctggc catcaactgg atgtaccggg acgaataccg gcgcggcggt tttgtcatgc
    14221 tggcgaacga ggacgaggaa ggcgttcgca cctcgcgcca cgcgctggcc tactcgttgg
    14281 ccacgctgct gctcatgttt tatcctgtgg cgcgcggcct ggtgaatccc tggctgttcc
    14341 tgccgcccgc cctgctgctg gccggctggc tgtgcaagct ggcgctggtt tttcaaaaat
    14401 cccccgatcg ggtcaacgcc cgcaaacttt ttttctgcac cctggcctac ctgccgggaa
    14461 gtttgatcgt aacttgcctg gcctggaaac acggttaatg cccgtgagaa ccccgggcac
    14521 gcgcgccccc tgttaaacat gtccgaccaa acccgccctt tcaatccgct cgccatctgg
    14581 ataccaatcg tcatgatggc gctcggcgtg gtcatttatt acaactacct cgtctatgtc
    14641 caggagcagc agcgcggcga ggacaaaaac cgcccggcct atctcggccg tctggagcgc
    14701 gacatcgagc tgaccgagcg agacgggcgc aaagtgcggc tcgacgcgct gcgcggcaaa
    14761 atcatcctcg cctcatgggt ttacacgcgc tgcccccgcg gctgcgctgg agtcatcgcc
    14821 aagctcaagg ccctgcatga cgagcatcaa ggcaaccccg ccatccatct cgtctccttc
    14881 accctcgacc ctgatgacac gccggaaatg atgcaaacct tcgcgcgcgg catcggcatc
    14941 gccgacgacg ccaactggtg gttcgtcaac ggtgacaagg acgaggtgcg ccgctacatg
    15001 acccagtact tcaagttccg ccccgtgcag gatctccccg agggggaccg cctcacgccg
    15061 gacgacaaat acattcacga cctgcgcgtc gtgctcgtgg acgcgcaggg gcatgtgcgc
    15121 ggatcgcttt acgacctcat gaacgcggat ccggagttcg ccaaatactg ggacgcccag
    15181 ctccgcaaag acctggcctg gctgctcaag gaaaaacccg cgcagcccgc cgacgctcct
    15241 gaacccaaaa actgaccgcc atgtctgttt acgaactgcc caagctctac accctcttca
    15301 atgcctgcgc gctgctgcac atcatcctgg gcctgttcat gatccgcctg cgccagccca
    15361 aggcgcacat cgccagcatg gttatagcgc tgatgttctc caccgccttt cttggctgct
    15421 acctgtatta ccactaccac gccgggcatg tggtttttca ggggcgtggt ttcgcgcgcg
    15481 tcttttattt caccattctt ttcacccaca tcccgctggc cgtcatcaat ctgccgatga
    15541 tcatcctgac ggtcgtgccc gctgtcaggc accgttttga caggcacaag cgcatggccc
    15601 ggtggactgt cccggtgtgg atctatgtct ccgtcacggg agtcatcatc tacctgatgt
    15661 gctacgtctg gtatggtccg ccgctccgga gctgacctgc atcaacgatg aggggttcgt
    15721 tgacagttgt ccgcaggctc ggcgttctcc ggtgttgatg atatcgtggt ccgcctgatc
    15781 gaggacctca ttatcacgca ttccatttct ctcaactgat gaaaaccctt ctccactgcc
    15841 ttttccttct cgcgcccggc tttctgctcg cggagttgag cgtgccgcga ttcttcagca
    15901 accacatggt gctccagcgc gagcgcgcgg cggccatctg gggcaaggcg gacgcgggag
    15961 ctgagatcgc gctgtccttc aaaggcaagt cggcaacagc gaaggctgga gcggatggca
    16021 gatggcgcgc gcggatcgaa acaggcgccg ctgacgcaac gggggccgcg ttgacgatca
    16081 ctgccggctc tgaaaagatc gaaatcaaag acgtgctcgt cggcgaggta tggttcgcct
    16141 ccggccagtc gaacatgtat tacaccatga accgctcgcc ggagtatgag gggctcattg
    16201 cggagtcgaa tcatccggcg ctgcgcatgt tcaatgcgcc gctcgtcacc gcagcggaga
    16261 accaggacga catcgaaggc gcgtggcagg ccagcacgcc ggaaacggtg cccggtttct
    16321 ccgccgtggc cttcttcttc gcccggaagc tgcatctcga cctgggcatt cccgtcggcg
    16381 tgatcaaatc agcctggggc ggcaagccgg tggagacttt caccagccgc gaggctttga
    16441 acacgctgcc aggcacgaag gcactcgttg aagtgatgtt gaaggacgag gagggttacg
    16501 atcaggagaa ggcgaatgcc gcttacacgg ccaaactgga gcagtggcag gccacgatgg
    16561 ccgccgccaa gggcaagtcc gcagaggaac gcaggcgcct gccgaagaag cctgacgcgc
    16621 cgaagcgtcc gctgctcacc gaaggcaggc cgggcgtgct attcgcctcg atgatccacc
    16681 ccttcaccgg ctacaccctg cgcggtgcca tctggtacca aggcgagggc aatgcgaagg
    16741 cgggggccgt gccatatgac cagacgctgc ccctcatgat caatgactgg cgcaggcgct
    16801 ggggcgatga gttctccttc tacttcgtgc aactcgccag ctatcgcgca ccctccaccg
    16861 agcctggcca gcccgatccc tgggcgctct gccaggaccg catgcgccgc gtgctcggaa
    16921 ccacgccgaa aaccggcatg gcagtcatca acgacgtcgg cgaggcggat gacatccatc
    16981 ccaaaaacaa aaaggacccc ggcgagcgtc tcgcgcgctg ggccttgtcc gctgactatg
    17041 gccgcgagat ggttgtttcg ggtccgcttt tcaaggaggc gagcttcaat gagagcgagg
    17101 tcctgatcag cttcgaccac tgtggagagg ggctcaaatc ccgcgatggc ggtccgctga
    17161 agcgctttga ggtcgctggc gaggacaaag tctggcattg ggcggacgcc agtgtcaaag
    17221 atgagttcac agtcagtgtc agcagttcga aggtggggaa gcccgccgct gtccgctacg
    17281 cctgggcctc gaatccggaa ggtgccaatc tcgtcaacag cgagggtctg cccgcttccg
    17341 ttttccgcac ggatgattgg gatgatgtgg aggtcaaagt ggacaccaca gcggctgatg
    17401 ccgccgccgc ccgccgcgct ctcgcctccg agatcaaagc cctcgccgcc gagcgcgcca
    17461 agcacgaccg ccaaagcccc gaatacaagg cgctgaacag gcagcttcaa gaacggatgg
    17521 caagattcaa gaaaggcgct gaaaaaaagt gagcctcccc gcaaaatttt tgtccagaaa
    17581 gcctctcgtc cttccgagca agggcaacaa tgtcagactt caggccacgc agtcacgagc
    17641 agttcatgcg cttgttcatc gcgagcgagc cggaggtgct gcgctacatc ctggctgtca
    17701 ttccgaacgt gactgacgcg cgggatgtgt tgcaggagac ggcggcggcg ctttggcgga
    17761 agatggatgg gtatgatccg gcggctccgt tcacgccctg ggcctgccgt ttcgctgcga
    17821 atgaaatccg cgccttcctg cgccgtgagc atcgccagcg gcgctggctg gacgcggatg
    17881 tggcggagct gctgcgggtg catcaggagc aggcgctcca gagcgatgac ggcgcggcgg
    17941 agcatctgcg cgagtgcctg gagtcgctgc cgcccgcgca gcatgagctg ctgcggcgct
    18001 actactttga ggagcagccg gtggaggcca tcgcgcgggc gctgaaccgc acggcggacg
    18061 cggtttacaa gactttgcaa aggtcccggc aggcgctggc ggattgtctg aaacgcaagc
    18121 aggaggccgc tgcatgaggg agctggccgc gcgttatctc aatggcgccg ccacggaggc
    18181 ggaggtggcg cggctttccg agctgctgcg cgctgaccaa ggcgcgcgtg aggaatatct
    18241 gcgcctcgcg gacgtccacg cgcgcctggc cgccgacact tcattgtgga cccatgaacg
    18301 gccgcgcaac cagactgttc ctcgctggaa gggctggctg ctgcctttcg cggcagcggc
    18361 ggcggtgctg ctcatggcgg cgcttttttg gcctgcggaa aaggcagcgc cgccacccgt
    18421 ggccacgctg ctgcgcgccg aggatgccgt gtgggccggg ggctccccca atgaaggcgg
    18481 gcgcctgaac gcggggcctc tccgtctgag cgccggccgt gcgctgctgc gtctggatgg
    18541 cggcgcggtt ctgcttctgc atggcgcggt ggatgcgcgg ctcgaaagcg gaggcagcgt
    18601 ggctttgctc gccggtgaaa tccacgcgca ggtgccggag gcggcggcgg gcttcactct
    18661 ccgcgcgccg ggtggcgacg tggtggacct cggcacggag ttcgtcacgc gcgtgtctca
    18721 tgatagcatg gccgaggtca cggtggtgaa gggtgaggtg gaggtgcgtc cccgtcaggg
    18781 tccggcacaa cgcctcacca ccggcaaagc gctcgccatg gagcgtgatg gaagcgtgcg
    18841 cgccaccgcc gccaggctgc ccgtccgcgc ctgggaagat tgggagatca aaccggcggc
    18901 gcaacgacgt gaaggcgcct tgctagtgca tgacgcgttt gaatacccgc ccggcagcca
    18961 cgatcccgcc gctctgactg gcggcgcggg ctggggcgga ccgtggtccc tgctgacgga
    19021 ttcacaaggc gcgcgcatct actcaggcgc ctcgcgcacg atggagacgg gcgccttcgg
    19081 caacgccggg gcatggcttg cgcccgccgg aaaaaacctc cgccagcgcc gcctcgcgca
    19141 gccgctggac ctcgcgcagg acgcggtgcg ctacgcaagc ctcgcctggt ttgaagagtc
    19201 gctgccgact gggcggcgaa agccatccgc ctcgcctgcg agcgggcttt cgctaacttt
    19261 ccgcagcctg gaggatgccg cgccggggcg tgtcggcctg cgggtggatc atctgctgcg
    19321 cccgcgcatt gagacaggca tggggcaggg ctttgtgagc cgcgtacgcg ccaacgaggg
    19381 ccgccgcctg ctgctggtgg caaagatttt gtcccgccgc gaaggcgagg atgagatcat
    19441 gctgcgcgtg ttcgacgcgg acgatccgcc cggcgcctgg gaacctgagg agtgggacat
    19501 ccgcacgcgc ggcctgcgcc tggatgccgt gttggaccat gtcatcctgc aatccagcgg
    19561 cccaagcccg cgccgcatcg aggaggtgcg cctcgccagc gcctggctgg atgcggtgac
    19621 cggatggagt gctgaaatgt cgaatgacga atgacgaaga tcatgaaccg gcccgcctta
    19681 ttcctgctcg cctccgccac cgccctcgtg gcggaggaca tggctttttt tgaaaccaaa
    19741 atccgccccg tgctggtgga gaaatgccac tcctgccaca gcgccggcgc ggaaaaggtg
    19801 aagggcggcc tgctgctgga cacgcgcgag ggcatccgcc gcggtggtga ctccggccct
    19861 gccgtgacac ccggggaacc cgcgaacagc ctgctgctca ccgccatccg ctaccaggac
    19921 cgcgacatgg agatgccgcc gaaggaaaag ctgcccgcca gtgtcatcgc cgattttgag
    19981 aaatggatcg ccatgggcgc gcccgatccg cgcaatggca gtgccgccgt gatgcccccc
    20041 accgtggaca tcgaggaagg ccgcaagcac tgggcctttc agccgatttc aaatccgcca
    20101 gtgccgcagg tcaaagacga gtcctggccg cgcacggaca tggaccgctt cattctcgcc
    20161 gcgcttgagg aaaaaggcct gcgccccgtg gcggacgccg tgaccgaggt gctgcggcgg
    20221 cgcatcgcct tcgatctcac cgggctgcct ccagatcaga ccgatccgac tgatccgtca
    20281 gatccgaccg atctccttaa tagcccccgc ttcggcgagc gctgggcgcg gcactggctg
    20341 gatgtggcgc gctacgccga gtccgctggc ggcgggcaca acgtgctctt tccgctggcc
    20401 ttccgctacc gtgactatgt cattgacgcg ctgaacgcgg acacgcccta cgaccagttc
    20461 atccgtgagc agctcgccgg ggatcttttg cccgcgcgca gcgatgcccg gcgcaatgcc
    20521 cagctctccg ccaccggctt tctctgcgtg ggcgtgaagg acctgcgcga gaacagcaac
    20581 caccgctacc gcatggccct ggccgacgag cagatagacg ccaccagccg cgccttcctc
    20641 ggcctcacca tcgcctgcgc caagtgccac gatcacaaat tcgatcccat ccccaccagc
    20701 gactactacg cgctggccgg catcttcacc agcagcgagc ccctgctcgg cgcgcgccgc
    20761 aaccgccagc gccatccgct cggcgccggc ctcgcgccca tggccggcgc gcccgtcgtt
    20821 ttcagcgatg ccgactttgc gcaaatgctc aagctgcgcg tggacgccac ctatgcgcgg
    20881 ctggccgtgc gcgatgagaa ataccgcatc ctcattgaaa aaggcctcga ccggaaaaac
    20941 acggaaaagc acaacgccat gctggaggcc atcccctccg tccaaaagct catgcgcgtg
    21001 gtggaggaca ccgaggccgc ctgcgaggcc ctgcgcgtgc gctacgacgc cgccctgccg
    21061 cacagcctca tggccatgcg cgaggacaag ccggaggact gcgccatcca catccgcggc
    21121 gaggacaccc agctcggccc ggtggtgaag cgcggattcc cgcaggtgct ggccaccgtc
    21181 gagaccgcgc ccgtgccgca ggaccaaagc ggccgcctcc aactcgccga atggatcgcc
    21241 agcccgcgcc atccgctgac cgcgcgcgtc atggtcaacc gcatctggca gcatctcttc
    21301 ggcgcgggcc tcgtcgagac gccggatgac ttcggcctca ccggccagcc gccgtcgaat
    21361 cccgcgctgc tcgaccacct cgccagccgc ttcatcgcgc acggctggag cgtcaagaaa
    21421 atgatcgccg aaatcatggg cagccgcgtc tatcagctca gcacctccca cgacgcggag
    21481 aactacgagg ccgaccccgc caaccgcctg cactggcgca tgaaccgccg ccggctcggc
    21541 tctgacgcgc tgtttgacgc cgtgcgccac atcagcggcg acctcgcact ggagcgcccc
    21601 gcgcccgtca ttccgcccac gccgcaggac gaccgtgtga aatccatgga cctcaaagcc
    21661 tggttcgcgc ccaccgcgcg ccaccgcacc atctaccagc ccgtgctgcg cgaccatgtg
    21721 ccggaggact ggaagctctt cgatttcccc gaccccgaac tcgtcaccgg ccggcgcgcc
    21781 caaaccaccg tgcccaccca ggcgctgcac ctgatgaaca gccccttcat cgccgaacat
    21841 gccaggaaaa ccgccgcgct gctcctgccc tccgccacca gcaccgaaac cctcgtccgc
    21901 gccgcctgcg agcgcatcct caaccgcgcc ccgacccctg acgaaatccg cgaagccgcc
    21961 gcttttctga aatccttccc cgcaaacgac aaatccgaat ccgccgccgc gctctgccag
    22021 accctcttcg gctcagcgga gtttctttat ctctactgac gcatgaaccc accccctttc
    22081 tccctctccc gccgccgcat gctgcgcggc acgctaagcg gatttggtta cgccgcgctg
    22141 gcaggaatgt gcgcgcgagc cgccacgcgc gagccgcatt tttctcccaa agccaaacgc
    22201 gtcatcctgc tctacatgca gggcggagtc tcgcacctgg acacctttga ccacaagccc
    22261 aagctccagg ccgacagcgg caaggaatac gccaccggca agggcggcag gctgctggga
    22321 agcccgtgga agttcaaaca gcacggccag agcggccagt ggatcagcga gctgtttccg
    22381 cacctttcca aacgcgccga cgatctctgc gtgcttaccg ccatgcacac cgaggcgcag
    22441 gcgcatgaga cggccgtgcc catgtttcac acaggcaacc cgctccaggc ccggcccagc
    22501 ctcggcgcct gggtgctcta cggactcggc gcggagacgg aaaacctgcc gggcttcatc
    22561 gctatgaact cgctgcgcca gtttggcggc aacaaccacg gcagcgcctt tctccctgcg
    22621 tccttccagg ccacccacgt caaacccggc ggcaaggacg gcggcggcga tcccgtggcg
    22681 aatctgaaag ccgcgcacct cacgccggag cagcagcagg cgcagcttcg ttttctccac
    22741 agaatgaact cgcgccgcct cgcgcgggac ggcgtcaacc atgacctgga ggcgctcatc
    22801 agcagctatg agatggcctt caaaatgcag agtaccgtgc cggacctgat ggacctgggc
    22861 agggaaagcg aggccacgct caaagcctac ggcatcggct ccacagccac cgacaccttt
    22921 gcccggcagt gcctctacgc ccgcaaattc gccgaggccg gcgtgcgctt catcgaaatc
    22981 ggcaccggcg gctgggacca ccacaaggac ctcaaggccg agatgacccg cagcgccgcc
    23041 gccgtggacc agcccatcgc cgcgctgctg gccgacctgc gtcagcgcga cctcctgcgc
    23101 gacacgctcg tcgtcttcgc cggggagttc gggcgcacgc ccttcgcgca gttcgacaac
    23161 gggcgcgacc acaacaaccg cgccttcacc gtctggatgg ccggcggcgg ggtgaaggcc
    23221 ggccaccgcc acggcctcag cgacgactac ggccgtgagg gcgtcgaggg ccgcgtccac
    23281 atccacgatc tgcacgccac cctgctgcac ctcctcggcc tcgaccacga acgcctcacc
    23341 taccgctaca gcggccgcga cttccgcctc accgatgtga aaggcgaggt ggtccgggag
    23401 gtgctggcgt gaatgatctt catgttccga gtcaggctag ccacaaccct ctcatgaaac
    23461 cgctcatcat catcgccatc atgctggccg gcgtcctccg cgccgcgccg ccgaacattc
    23521 tgctcatcct ggcggacagc cagacgaccg ccctcggctg ccatggcgcg ccccatgtgg
    23581 agacgccgaa cctggacaag ctggccgcgc gcgggctgag cttcacccgc gcctggtgcc
    23641 agcacaccat cggcagcgcc tcgcgcgtga gcttcctcac cgggctgcgc ccgcgcacgc
    23701 tgggcatcag cggcaactac cgctacgcgc ccgatctgcc gaaaaacgcc gccacgctgc
    23761 ccgcatggct caaagcccag ggctggcgca caagcgtggc gggccgcgtg tttccgcgcc
    23821 ggtctgacta cgccacctca tgggatgaaa tcatgctcgc gccggaggac gagggtgaga
    23881 atgcatccgg catgaccacc gtccttggcg tgcctgtgcg gagcgtggag gtggatggca
    23941 aaaaagagcg ccagggtgat ttgctcgttc tgcgcagcgt gaaggccggg gacgacggca
    24001 cgccagacgg gcgcgtcgcg cgcgaatgcg tggcgcagat ggaaaaagca gccgccgccg
    24061 gcaaaccctt ctttgccgcc gtgggcttca tgactccgaa cttccgctcc ccggtgccgc
    24121 aggcctggct tgaaaagttc aaggacaggc ccgcgcccga tccgctgcct ggcccgcagg
    24181 gcgcgccgga gtgcgcgcag ttcggcggca tcctgccgga gcgccaggtc acggaggagg
    24241 agcggcggca gttcatcgcg ggctaccgcg cgcagagcgc cttcatggat tcgcaggccg
    24301 gcctggtgct ggatgcactt gaaaagagcg gcctgatgga aaacaccctc gtcgttttca
    24361 ccagcgccaa cggctatcat ctgggcgatc atggaaacct ctggggatcg cgtacgcttt
    24421 acgccagcgc cgcccaggtg ccgctgatca tcgccggccc cggcgtggcc gccgggcagg
    24481 tgtgcgagcg cgtggtggag atcatggacg tgtatcccac cctgatggag cacctcggca
    24541 tgaaaagccc gcgcgtcatg gatggcgaaa gctttgccaa gctgctgaaa aaccccgccc
    24601 tgccccatga tgatcccgct ctgaccagcg tgctgaacta cggcccgcac agcggcgtgg
    24661 cagtgcgcac cgcctttgcc agcttcatgc gctggggcag ggacaccgcc gagctttaca
    24721 atctgcagac cgatccgcac gagctgcgca atctggccgg cgacaagtcc atgtcctccg
    24781 cccttagaaa aatggaggcg ctgctggaaa aggaagtcgc caggtagcag gcgcatcatc
    24841 aaactcggcc atgtcagcac gccgcagatg cacgtttatc ttccgccaaa ggaaaaggcg
    24901 aatggtggcg cggtgctcgt gtgttccggt ggcggctttt ccattctcgc ctgggatctc
    24961 gaaggcacgg aagtggccga atggctgaac tcgatcggtt ttgccgccgt cgtcatgaag
    25021 tatcgcgcgt ccaccgcgca tcatggcaac aatttgaacg agcaaggcaa cgcgtcgctg
    25081 aaggccgtcg gccccttgat ggacgcgcag cgtgccatga gcctcacgcg ctcgaatgcc
    25141 gccgactggg gccttgatcc gcagcgcatc ggcatcctgg gattctgcgc tggcggcgaa
    25201 acggcgggac tcacggcgat tctacgcgat caacggctct atgcgaaact cgacgcagcc
    25261 gacgaatact cctgcgcggc caattttgcc ctgccgatct atcccggcgg cttctacggc
    25321 aaggcgaccg gcggtttgaa gccctatctc aaagtcacga aggacacacc gcccatgttc
    25381 ttcgccatgg cgcaggatga ccatgtgaac tcgctgaact gcaccgtgct ctacaccgcg
    25441 ctctcgcagg cgaaagtgct ttccgagctg catcttttca tacgaggagg gcatggctat
    25501 ggattgcgtc ccacactcac gccgttcacg cactggccga atcgggctgc gaaatggctc
    25561 aaggaaatgg ggtgttgtga agcgtcgtcg gattgggcta aagcccaaac tactttggga
    25621 aatcccgccg attatctccc gcgcaacgtg atgcaaatca ccgccttcgg cgagcgggcc
    25681 gagttttcgc acgacagcca gcgcgtgctg ttcctgagca aacaatacgg cgacgtcatg
    25741 gaatacacga tccgcagcgg ccagatccgc tgcctcacgc agcacttcaa gcatcacggt
    25801 ttcaatcgcg tcatggtgct gagcaatggc gactacctgc tcaccggtcc tgacgaaacc
    25861 ttcgattcca ccagcaaaga ggcacggctc aaagcgcggc acttcgcgaa gatgttcgtg
    25921 ctcgaccgca cactcaccaa gccaccgaca ccgctggggt cgtcgccgat gaagggcctg
    25981 ccgtttctag ccagaaactc aaaatcgctt ggacgcatca catcgagggc gggcatcgcc
    26041 agaccgcgat ctcgatgggt gatattgttt atgaaaacgg cactcccaag cttgccaatg
    26101 tcgcgctcgt actcaccacg aaggatttcc ccgaaggcca gcgaccaaaa atgatcgagg
    26161 cgcataactt cacgcccgat gacaaggcga tcaccctcac cgcctacctc atcgaaggcg
    26221 gccataacac cgagggctac actttagatc tcgaatcaaa gaaactcacc aacttcaccc
    26281 gcacgccgga cgactatgag gaagtcgagg gcatctttcc cgacggcaag agcactctcg
    26341 tcgagcgcaa ccacagcgtc ggcaagccgt ggccgatggt ggatgcgtgg cgcgtgtggt
    26401 tcgacggctc caaagaaccc cagcgcctta cccacttcct ggatttcaaa ggctacaaag
    26461 ccagcaacta cgtcgtcagc gacgatggac gtctcatcgc cttccagctc ggcatcagcg
    26521 gcgacgaggc tggcgtgggt tatggcatct tcttgatgga gataaaagcg cgcccctaag
    26581 gcgctgtcgt tgtgcgactt ttaccaggat agttggccaa ccggttcaag acggtggcat
    26641 tgaatgtcgt caacgtgcct tcaacgtgaa acatgccgca tccgctttcc tcgccatcct
    26701 cgtcatgagt ctgtctttac ctgtggctgc ggatgatcac gcggagctgg cggctctcag
    26761 gcagcgtgcg gagaaacgag atgccagggc gcaactcggc ctggcgtatc gttatcgcga
    26821 tggcaaaggc gtggccaaag acgacgtgga ggccatgaag tgggcgcaca tggcggcgga
    26881 cgcgggcaat gcggatgcga tggactttgt cggttttgtt tatctgcgag gggcggtggt
    26941 ggagcggagg ccggagatcg cgtttggcta cttcaaagcg gcggcggatg aatcggcgca
    27001 ggcggctttc aatctcggac agtgttactt tggtgcccag ggcacggagc aggattgcgc
    27061 gaaggctctg gaatggtggg agaaggcggc ggcgatgggc catggtcgtg ccgcggccac
    27121 ggcggcgatg gcataccatt cgggtgaagg cgtcgcgatg gatgcggtta aggcgcggcg
    27181 tctcgcggag cgggcggcgg aactgaacaa tcccggcggc ctcgtcgtgc ttggtgaaat
    27241 gcaatttcaa gccggtgacc tcgatgcggc gcgcgcgagc tggacgcggg catcgaaact
    27301 gcaacccaca tcggccaccg ggcatccttc gcaaccgtct gacaatgcct ccgcgcagca
    27361 gggcgcggat cttttgaagc tgatcgacta tcggaagagc aatagcgaac ccggcaagtt
    27421 cgccttcgtg caaatgcctc acatctacca gggctacaac aactgcggct cgaccgcgtg
    27481 cgccacgttc gcgcgctttc agggcagtga aattggcggg tgggactaca agaagctgtg
    27541 cccatcgccg ctcggcaccg gcacggactg gggtcacctg ctcgatgcat cgaagaagat
    27601 cggccagaaa tggaagctca tcaccttcac gccggatgac gcgggatttg gagaggccac
    27661 agcctttttg agaagcgaac tcgatgccgg acgcccggtg gtcgtggatt tcaaatacat
    27721 cggcccgcaa taccccaatg gcagcgccgg acacacgctc agcgtgtgcg gctacattgc
    27781 ggcggagaac ctttacatcc tgtgcaaccc ggccgtcgcc acgcccggcc tgcaactcat
    27841 caccgccgac gatttgaaaa acttctggcg gtccgatcac tacggcgcgc tgtcgaaagg
    27901 tgtgctctcg cgtcccgcgt ttgtcattga tcgttcatga agtccagcgt ggtcattctt
    27961 ctcgccgcca tgctgccctc tttcgcggca gcacagccgc cgatcatcgc cgggatcgaa
    28021 aaagcgggcg tggagaacgt cacaggcgcg gtcctgatga gcgagctggc ttgcgcggcc
    28081 tgccatgcgt gcatgcagac agtttttgcg ccgaaaaccg cacccgattt gtccgcgatc
    28141 ggctcacgcg cgcatccggc gcatctgcgg cagttcatcg cgagtccatc gggtgtgaaa
    28201 ccgggcacga cgatgccgga tgtgctggcg cacctgccgg aaaatgagcg aaacgaggcg
    28261 gcggaggcgc tgacgcattt tctcgcgaca cttggcaagc cggcaccatc cgcgttgccc
    28321 acggcggagg ccattgagcg cggaaagggg ctctatcact cggtgggctg cgtggcctgc
    28381 cattcgccgg agcagccgtt gcccggctct gtgccacacg ggccattggc ggagaaatac
    28441 acgctcgcca gcctcaccac gtttttgctg gacccgctcg ccgctcggcc cggtggacgc
    28501 atgccggact gccagctcga aaacggcgag gccgcggaca tcgccagtta tctgctgcgc
    28561 gaacaaaaaa cgccgctcgc tccttttcag ccagatgccg cgctcgccgc acgcggtcaa
    28621 aaactcttcg cggagcatcg ctgcaacgca tgccacacta ccggaaccaa aatcgagccc
    28681 ccatcgctca ccgcgctgaa caagctgcga gctgatgaag gctgcttgtc gtcaaaaaag
    28741 ggcgcgtggc cgcattatcc gctctctgag gggcagcgga cggcattgcg atccgcgctg
    28801 accgatgaag cgaagagctt ctcaccggct gaagtggcgc aaatcaccct cacgcggctg
    28861 aattgcatct cctgccatgt gcgcggagaa atcggcggtg tctccaccga gcgcagcgac
    28921 tacttcaccg gacgcgatga aacgctcggc gagcaaacac gcatcccgcc cgcgctcacc
    28981 ggcgtgggag ccaagttgaa ggagaaatgg ctgcgtgaag tgctggtgaa cggcgccagc
    29041 gcgcgtccct acctgcacac acgcatgccg agattcggcg cggcgcagac ggcgggcctc
    29101 gtcccgcttt tcaaacagct cgatccgccg ctgacggccg cattcacgcg tgtcgaggct
    29161 gacaagaagc ctcgtgagat cggacgcgag ctggcgggca cgaagggctt caactgcatc
    29221 gcctgccaca ccttccgcgg ccgcagcgcg tcgccgattc gcgcgcttga tctcaccacg
    29281 atggcggagc gcgttgagga aaactggctc catcacttcc tcgcgcatcc gcagaaattc
    29341 gtgccgctga ctgtgatgcc cggcttctgg ccggatggca aatctcttct gcccgccatc
    29401 ctcggtggcg atccgggcat gcagcgcgac gcactgtggc aatacctcgc gcaaggccca
    29461 gaggcgcgcg aaccagtggg cctcgtgctt gaaccgctcg tggtgaaggt gctgaacgag
    29521 gctgtgatcg tccgccgtgc tttccccggc atcgggaaac gcggcatcgg catcggctat
    29581 cctggaggca tcaatctcgc gtttgacgcg gagttgatgc gtctcgcgac gctatggagc
    29641 ggcgacttca tcgaggcctc gtcactctgg cgtggtcagg gttccggaca ggcccgcatc
    29701 cttggaaaac cggtcgtgga tctcccaccc ggccccgcgt tcgccgtgct cgcgacagca
    29761 gatgccgcgt ggcccgcgct cgacatgacc accgagagat cgccgttctc gttcaaaggc
    29821 tacagcctcg atacgcagca gcgccccacg ctgaaatatg cgctgcccgg cgcggaagtg
    29881 acggatttct tcatggaacg ccgcgatgac tccggaagcc tctacctgga gcgcacactc
    29941 actttttcat ccccaccacc tgacggattt catctccgcg tggcatcagg caccatgatg
    30001 gaagctcaag gtgccaacac ctttgctgtc ggcccgaatc tcgtgatcaa gatgtccgcc
    30061 cgagcggtgc ttcgcgatgc gggagatgcc aaagagctgc tgctgccggt gcgcggagat
    30121 ttgaaactcg aatatcacct caagccggca tcaaccgaag gaaaggggcg gaaaccatga
    30181 agcgattgac ccaaatcctc attgtctgcc acctgctgat cgctcctgca ctgctggccg
    30241 aagacctcgg cgacacctgg ggcacggcgg atcgcgaggc ggccttttat cgcatcgtga
    30301 atgtgccgat gccggaggat gtgtatgtcg aggcgggcag ttttgtctcg ctgcctgacg
    30361 gaaggctggc cgtgggcaca cgacgcgggg agatcttggt cttcagcggc gtctcggctg
    30421 acactccgaa gccaacggtg agcacgttcg cgagcgggct ggatgaagtg ctcggcctcg
    30481 cctggaaaga cggcgccttt tacgccacgc atgcgaccga ggtcacgcgc atctccgata
    30541 cgaacggcga tggcagcgcc gaccactacg acacgatcag tgatgcgtgg ggattcggtc
    30601 attaccacga gtttgcgctc ggatcgaagt tcgacgcgtc gggcaatctg tatgtcgcgc
    30661 tcggcctgtc ggagtcgtat cattcgaagg ccctgtttcg cggctgggcg ttgaaaatct
    30721 cgcctgatgg caaaacgacg ccgctttgca gcggcatgag gagcccgctc ggtgtcggcg
    30781 agaatgaaag cggcgagatg ttttatgtcg agagccaggg gccgtggaac ggctcctgtt
    30841 cgctgaagca tctgaagccc ggcggcttca tggggcatcc cgtcagcttc aactggtatc
    30901 cgcacgcgcc ggagatggga cctgtgcccg tgatgccgaa cacgccgtca cgcatggaga
    30961 ccgagcgcaa acgcgtgaag gaactggtgc cgtatgcggt ggtctttccg tataaaaaaa
    31021 tgggccgctc catctccgcc tttgaggtgt gtcgtgcgcg cgggaagttc gggccgttcg
    31081 acgatcaaat cttcgtcgga gactacacat tgagcgtcgt gatgcgtgcc acgacggaga
    31141 aagtgaacgg cgtatggcag ggcgcgtgct atccgttccg cgaaggactt ggcaccggca
    31201 tcctggccgt gcatttcacg caggatggca aactcatcac cggcggcacg aaccgcggct
    31261 ggcccgtgcg cggcatgaag gacaatctgc tccagcgcct cgactggacc ggccgcgtgc
    31321 cctttgaaat ccgcgacatc cgcgcccagc ccgatggttt caccattcgc ttcacacgcc
    31381 ccgccgatgc cgcccacgca tcgaaaacgg aaagctacca gctcgaaacc tacacgcaca
    31441 tctaccagca aggctatggc agccccgaag tcgatcacac ccgccccacc gtcaccgccg
    31501 ccgaggtcgc ccctgatgcg atgagcgtgc gcctgcgagt caaaggtctc atgcaaggcc
    31561 acgtccacga ttttcatctg ccggacttca agtcgcagga tggcgaaacg ctgctgcacg
    31621 accgcgccta ctacactctc aatgagatac ccaagccatg agactgacag aaaaaagaag
    31681 gcagaaacag ggctctgatt ttttctgtcc ctttctttct gccagccatt ccgactcacc
    31741 atcaccatga aacccatcgc catcagcctg tttgcgcttc ttgcgttctt ttgcagccat
    31801 gcattcgcta cgccaaacat cctcgtcatc ctcgccgatg acgtgggctg gggcgatgcg
    31861 ggttgctacg gcggcatgct ggtgaagaca ccgcacatcg accgccttgc tcgcgaaggg
    31921 cagcgctttg tgaatggcca cgcctccgct gcggtttgca cgcccacgcg ctactccatg
    31981 ctcaccgggc agtattcgtg gcggcaaagc ggcgcgggtt tgaacaaagg cgtctcgaat
    32041 ggcgactcgc cgctgctcat ccccaccacg atgaccaccg cgcccgcttt gctgaaacaa
    32101 gcgggctacc gcaccgcgct catcggcaaa tggcatctcg gctttggcga taccccgccg
    32161 gattataacc aggagctgcg tcccggaccg ctcgaggtcg gctttcacga gttcttcggc
    32221 atccccgcca cgaatgaccg tctgcccacc gtcttcgtgc gcgatcacaa ggtgcaaggc
    32281 ctcgatcccg ccgatcccat ccgcctcagc tacgacaaag cgcaggccga gagccagggc
    32341 ctcaccgcct gggccgccgg gcgcagccgc atcggctggg gcaaaggggg caaggccgcg
    32401 tggtggaagg acaccgaaat ctccgacacg ctcactcgcg agtgcgtgca gttcatcgag
    32461 cgcaacaagg agcagcgctt cttcctgctc tacgcacctc acaacgtcca cgcgcccgcc
    32521 attcccggcc cgcgcttcgc cggcaccagc ggcctcagcc cgcgtgccga catgctgcaa
    32581 gagctcgacg cctccatcgg cgagctgctc cagacgctcg accgcctcgc cctagccaac
    32641 gacacgctca tcatctacag cagcgacaac ggcgcttatg tgaatgacga aaaaggccac
    32701 aagcccaccg gcccgtatcg cggcaaaaaa tcgcagctct gggaaggcgg ccaccgcgtg
    32761 cccttcatcg tccgctggcc cgcgtgcatc cagccccgcg tgtcgcagga catcgcctcc
    32821 accatcgacg tgcccgccac cgtctgtgct gccgcaggtg tcactgtgcc gaaggatgcg
    32881 ctgcctgaca gcttcgatct cctccccgcc atgctcggcg aaaaagcggc ctccaaacgc
    32941 gaccacctca tcctcatgag tggcaatggc gacctcgcta tccgcggtga ccgatggaaa
    33001 tacatcccga atctcgccgt cgccgacggc tggtatgcga acaaaaagaa aagcgccagc
    33061 gcccccgcac gtcccggtct cttcgacctc tccaaagacc ccggcgaaac gcagaacctc
    33121 atcaaagaac actccgccga agccaaacgc ctcgctgagt tgatggcgaa ggaccaggcc
    33181 agcgctgtga cgcggccgga ggggaaatag tcctgccgtt tcgagcatcc ttgaaaaaac
    33241 ttcgttaaat ccacgagccg tcggcagcga tggggtgaga acgaatcgtc tgaatcatgg
    33301 atgccgatca cacgcctgcc gcgccgcacg acccgaacga gggttttcta cgactgtgga
    33361 cgcatcatga gccggagttg cggacctttg ttcatgcctg cctgccgtgt gcggcggagg
    33421 tggacgaggt gatgcaggag gtgagcctcg tggcctggcg gaagttttcc acgctggagg
    33481 atcacggcca gtttccgcgc tgggcgtgct tgatcgcgcg ctacgagatt ctgatggccc
    33541 gacgccgtca tgcgcgcgac cgcctcgtgc tggacgagga catcgtcgaa aaacttgccg
    33601 aggagggcgc ggaggaaatg tccgagcgcc acaggcagct caatgcgctg gacgactgcg
    33661 ttgcgaagct gccgcgagag cggcgcgaac tggcgctggc ggcctatgcg ccggagactt
    33721 cgatgaagga actcgcggcc cgcctgggcc gcacggaagg ctctctttac caactgctcg
    33781 cacgcatccg gcaggaattg ctgcgctgcg tcgagcacac cctcgcgcag gaggcgcgca
    33841 tgtcatgacc caggaacaac tcgatctact gcacggctat ctgaatggca ctctcgatga
    33901 ggccggcttc gcccgattgc aggctctact ccgggaaaac ccggatgcgc gtcgaacgct
    33961 gcgcacgttt tccacggtgg attcaaagct ttatcagctc gcggccatca atcccgaaac
    34021 cgtgcggctg ctggcggagc ctgctggggt cagcaaaata tccacacgct ggctttcctg
    34081 gcgtccgctc actgcggcgg cggcggggct ggtgctcggg cttttcagcg ccacctgtgt
    34141 gtttgcgtat tcgcgactgt ctccattttc ggcagcagag gtcatggcgc cggcggtgac
    34201 gctgctggat gagggttttg aagcgggtga tgagcggttt cagacgggtt ttccggtgga
    34261 aacgggcgtt tggggtggtg atgaagttca ggtggtgggt gagagggatg gcatcacgcc
    34321 tgttgcgggg cagaggatgc tgcgcgtgga gcctgatgaa gcctccaata acagcaatct
    34381 cgaccgcatc ctggatcttt ccggcctgcc gatgcctgcg gacggtggca cgcgccatgt
    34441 ggaggtgacg gcctggtttc atgccgacga gccgggtctg cgaagccgct acatgctgcg
    34501 tgcggcggtg tttgccgagt ctgcgggaga tctgcgcggg cagtgggtga atgtgccgtg
    34561 gcaggagatg gagagccagg ctgtggcgct ggcgaagcgc ggcgtcagca ccacgccgga
    34621 catggaaggc tggcagaagc tcaccatccg cgtggaggct ccgccggagg cgcgcagcct
    34681 ggtggtctcg ctctccacac gcctggtcag aatgccggac cgccgcgccg cccaatacat
    34741 ggatgaggtg acggcggcgc tctggctgct tccaccgccc aagaagacca aacgcaacct
    34801 cctgccatga ccgcccgcac cctcctgacc tcgttgttgt tctgctttgc cacctcctca
    34861 atggcggcga atcccgctac ggaggtaaag ccgcccaaca tcgtcatcat cttcgccgat
    34921 gattggggct ggggggattt gtcgtgccac gggcatccgt ggctgaagac gccgcacctc
    34981 gaccggctcg cggcggaggg cacagacttc cagcagttca atgtgctcaa tcccgtctgc
    35041 tcgccgagcc gcgcggcgct gatgacgggg cattatccgg cgcggtatgc cattcatcag
    35101 cacttcgccg cgccgcagag caatgccgcg cgaaacatgc ccgactggct cgacgcgaaa
    35161 gcgccgacgc tgccgcgctt tttgaaacag gccggttacc gcacggcgca cttcggcaaa
    35221 tggcacctca ccaaccgcca cacccacggc gctccgaagc cggacgccta cggctatgac
    35281 gagtggagcg tcttcaacgg aggcgcggag tggccctcgt cggacggaca tgcctgcggg
    35341 cgcgacgccg cctcgttcgt ccgggcgaac gcgggcaagc cgtttttcat caatgcctgg
    35401 cttcacgaaa gccatctgcc ccatgtgccc acggcggcct cgatggaaaa atggaagcac
    35461 ctcgacccgc agcagcaggt ctatgccgcc gtcatcaccg atggtgacaa cgccgtcggc
    35521 ctcatcctcg acgcgctcaa ggatgcaggc ttggcggaca acaccctcgt gatgttctcc
    35581 agcgacaacg gcccggaaca cacggctgac aagcccggca gcaaggagga cgccgacgcc
    35641 ggaaccaagg gctacaacgg ctactacagc gtcggctcga ccggcggact ccgcggccgc
    35701 aagcgcagcc tgttcgaggg cggcgtgcgc gtgcctttca tcgtccgctg gccgacccaa
    35761 actccggcgg gaatcaagaa cgacacgacc gcgttcaccg ccgtggacct gctgcccacg
    35821 ctctgcgccg cagccgcagt cgccctgccg gaatcctacc gaggcgatgg cgaaaacctg
    35881 ctcgcagccc tccaaggcga gagcgtgaaa cgcacgcgcc ccattttctg ggaatggcgc
    35941 ggcggcggtg gagcggacca tgattggccc aacttgtccg tgcgcgatgg cgactggaag
    36001 cttgcgatga cccacgacga aagccgggtc gaactttacc accttgccga tgaccgcgcc
    36061 gagtcgaagg acatcgcgaa gaatcacccc gacaccgtcg cccgcctcgt caaaaaggcg
    36121 aaggagtgga aatccacgct gcccgccaac ccgaacgccg attgcatcag caccgccgac
    36181 cgcagccagg tcgcctcacc caaatcaaaa cgaaccaaac catgatacag cttctaaaac
    36241 ttctggctgc catcgttctc gccgcaaccg gcattggcgt gtttgcccat gacacagcca
    36301 ccccatcctc gcctggcggg gatcgcatca aggtgctcgt gtattccgac accgcctact
    36361 accgccaccc ggacatcccc ggcatcaatc gctggctggt gttgctcggc cacgagaacg
    36421 ggatggatgt ggacatcacc gagcacccaa aagacctcac gccgcaggtg ctcgcgaaat
    36481 acgacgtcct cctgctcaac aacgccaacg agctggacaa aatcatcccg gagcagcagc
    36541 ggcagtcggt cgaggcgtgg tttggcactg ggaacaaagg gatcgtcgga ctgcacgcgg
    36601 cactggtgcg ccagacaggc tggccgtggc tgaaccagct cggcggctgc gacttcaaca
    36661 gcgactccga tttcaccaag gcaaaggtaa cggtggatgc cgccgcaaag gagcatcccg
    36721 ccgtgaaagg ccagccggcg gagttttgga tcgaggcgga ctggacgaac catgaccgcc
    36781 cggtgaccgg gcttccgggt ttccaagtgc tgctgcgcgt ggacgagagc acctacacgc
    36841 cggtgcgcga ctatttcaca acacgaggcg gcaagccgat gggcgcggac catcccatcg
    36901 cctggacgaa tgaacctgcg ggtggcggac gcttcttcta caccgaattc ggccacgatc
    36961 tgagatcgct cgccacaccg tttgtgcgtc agcacatcct cgaaggcatt cgctgggccg
    37021 cggcgacaaa aaaatccgag gtcaaatcgg ccaagtgagc gctgttagtg gcagcgaaag
    37081 caacccgcga aaaccaagca atcctctcat tgaaactccc gctcaaattc caactccagt
    37141 ccttaccatg aacctgcgtg gtcttctttt cctcatcctc ttttcatgcg cgaccgcagc
    37201 cggcctggct cagaactccg ccgaagtcat cgtctacgat tcgacccctg ccggcttttg
    37261 tgccgccatc ggtgccgcgc gcgagggagc atccgtcatc cttctggaac cgacccagca
    37321 catcggcggg gtgaacaccg gcgggctgag tttgagcgac tcgttcggct gcatccgcga
    37381 atccttgggc gggctgtggc gcgagtggca cgagcgcatc gtcgccgact acgccgcccg
    37441 tggagtgcag ctcccgtatc aagtgaacga tgtgaacgaa gtcaaatgga cctatgagcc
    37501 gcatgtcgcc atgcgcgtga cccgcgcgat gttggacgag gcgaacgtga ccgtgctcac
    37561 gggccgctat ctccagtcgg tgaagaaaga cggcgcccgg atcaccacgc tcgtcacgaa
    37621 gaacggcagc ttcaccggca aggtgttcgt ggacgccagc tacgagggcg atctcatggc
    37681 tgcggcgggc gtgagctgga ccatcgggcg cgaagggcgc gacgagtttg gcgaatcgca
    37741 ggctggcgtg acccacggca tcaagaagcc cagggtgaag gtcagcggct tcgacgaaac
    37801 gggcaaccca ctgcccttca tcaccggaac gaaactcccg ccggatggaa cgccggacga
    37861 catgctccaa gtctttagtt tccggctcgt gctgacgcgt gatcctcaaa accgcgttcc
    37921 catgcccgcg ccagccagct acgacccggc gcgttgggaa ctcgcccgcc gattcatggc
    37981 tgtgcatccc gacaaggtcg gaattgatct ctacgaactt cccaacggca aattcgacgg
    38041 caacgatggc atccacaaac tgctgtcgct cggaatggtc ggcggcgctc aaaagtggtg
    38101 cggggctgac gaagcgggcc gccgcgccat ttgggaagag cacaagcaat acacactgga
    38161 gttttatcac tttctcagca ccgacccgtc cgttcccaag aaggcccgcg acacgtatgc
    38221 cgcgctgggt ctgtgcaagg acgagtttgc cgaacacggc cacttcccgc ccgaactcta
    38281 tgtgcgacaa ggccgccgca tggagggcga atacttcatg acgcaaaacg acatggatca
    38341 gagtccctcg aaggaagacc cggtgggcgt ctcctctttt cctgccgact cgcacgatgt
    38401 cacccgcatc gcgctgccag ccggtggcgt gatcaccgag ggggcctttc ttcgctgggt
    38461 gcagggaaaa cggcacggct acccgcatca ggtgccttac cgcgcgctca ttcccaagcg
    38521 tgacgaatgt gcaaacctca tcgtccccgt cgcgttttcc tgcacccatg tcgtcatctg
    38581 ttccgcccgc gtggaaccta cctggatggt tcttggccaa agcgctggag tcgccgccgc
    38641 catggcggcc aaacgcggcg cgagcgtgca ggatatgccc tacgcggacc tcaagccacg
    38701 gctcgaagca cagggacaac ggcttgttct tcccgaccaa tcaaagacac cgccacaaac
    38761 agcaaccgga ctcatcccgg tgacatcgct gccaggaatc gtgctcgacg acaaagacgc
    38821 tgaactcaca ggcgactggg cgcattcgac acgcttcaag ccgtgcatcg gcagcggcta
    38881 tgtcatcagc ggcgcgaagg atgcccgtgg cgatggcaaa gcctccgcga cctttcgctt
    38941 caaagcgccg aagtccggcg aatatcaggt gctcatggcc tactccgccc acgccacccg
    39001 cgccagcaac gtgcccgtgc tcatcaccag cggcggtcgc gaaacgaagc tcaccgtgga
    39061 tcaaacgcga cccctgcccg ccggtcagaa cttccagcgc atcggcaccg cggcactgaa
    39121 tgcgggagtc gagaccatca tcaccatcaa aaacacagac accaccggct tcgtcatcct
    39181 cgacgcggtg cagttggtgc cggtcaaata gcaaagcatc acccggaatc atgaaaacga
    39241 tcctccattc actcgccatc tcactgctgt ttgtcctcgc tgtccgcgct gaacagccgg
    39301 tggatgtgta tcgcacctgg acagacacgc agggccgcaa ggtggatgcg acttttcgcg
    39361 ggttcgagga tgggcaggtg ttcctgcaaa cgaaggacgg ccagctttat cgcttcgaga
    39421 tcggcaggct gggcgcggag gaccaaaagg cggctctgag catgaaaccg acgggcctgg
    39481 gcattccggc tgaccctgcc atcgccatgg cggcggcgaa ggtggacgcg ctggtggatg
    39541 cgggcctgcg ctcgaaaaac cagcgcccga atccgctggc gagcgatgag cagttcgtcc
    39601 gccgcgtgta tctggacatc gtgggccgca tcccgactcg cgatgaggcg ctggagctga
    39661 tcgaggacca aagtgtgtcg aaacgtgcaa agctcatcga ccggctgctg gccggcgacg
    39721 gcgcgaactc gcacctgtac aactacttcg ccgacatgct gcgcatccgc gacatcgcgg
    39781 gcaagctcgg tgtcttctac acctatcaag agtggctcaa gggcgagctg aagaagaaca
    39841 cgggctggga caagatcgtg catcagatgc tgaccgccga gggcaagctg ctggaaaacg
    39901 gcgccaccgg ttatttgctg cgtgatgaca acatgccgct ggacagcctc tcgctcacgc
    39961 tgacgagctt tctcggggcg gatgtcggct gcgcgcagtg ccacgatcat ccctttgccg
    40021 actggacgca gcggcagttc tatgaaatgg cggcgttctt tggcgctacc gacacgaatg
    40081 gcggcaaagg caaggatcgc ccgggcacca aggagctgcg cgaggccatg gccgtgatcg
    40141 cggaggagag caggcaaagg aaagagaaga atcccgatgc gggacgtgac aagtccgtca
    40201 agctggtgat ctacctggag cgcatcaatg gcttctccgt gtatgacgac gatgtcaacg
    40261 aactgaagct gccccacgac tacaaataca aggatggcaa gcccggcgac ctggtcacac
    40321 cgaagctcct gacctggagc aaggaggatg cgcgcctggc tgcgtatcgc gagttcaccg
    40381 ccacaggtgg cagggagccg gagcaactgc gggcgcagtt cgccaagtgg ctgaccgcgc
    40441 ctgacaatcc gcgctttgcc atgaccatcg ccaaccgcat gtggaagcgc gcctttggcc
    40501 tgggagtgcg ggagcccgtg tccgacctcg atgatcccgc agccgcctcc aacccgccgc
    40561 tgctgcagca cctggcccgc gagatggtgc gggtgaagtt tgatctgaag gccttcatgc
    40621 ggatccttta caacacgcag acctaccagc gcgaggccac gagccatgac attgacctgg
    40681 ggcagcccta cctctttccc ggcccgctcc tgcgccgcat gacggccgag caggcctggg
    40741 actcctgcgc cacgctcgcc gtcgggacgg ctgtcgatca gttccactcc gagcgcgcgg
    40801 aaaagtatgt gagcatcaaa aacatcaaca ctaacgccag ccccgccgag attctggagc
    40861 aggtcagaaa tgcgatcgcc cgcaatgctg atgcacccaa tgtttcaaag gagggcaaag
    40921 gggcaaagat cgcgatgcag accgccaaaa acctcaccag caccgacgaa gacggcgtgt
    40981 ccatcactcc gcccatgctc ggagagatgg tgatcgctcg tgccagcgag ctgctgcagc
    41041 ccgagcgcga atatcatttc ctgcgtcagt tcggccagag cgaccgccaa ctgagtgata
    41101 gcaacagcga cgagggccac attccgcagg tgctcatgct catgaacggc gcggcccagg
    41161 atgtcatcgg cagcacgcgc tcgctcgcgg tgcagaaggc gcaggagcag gccgctcccg
    41221 aggcgcggat cgagtcgctg tatgtgagct tcttctgccg caaacccacc gccagggaag
    41281 tcaccatcgc caaggagctg ctcagcagtg atctcaccct ctccgacctc gcctgggtgc
    41341 tcttcaacac gcgcgagttc atcttcgtcg agtaacaccg ccagccatca accaaaacaa
    41401 ccgcgctctt atttaccaaa ccatgaacac ccgcatcctt gatcccgagt cccgccgcgt
    41461 cttcatcgaa cgctgggcca aggccaccct cggcgtcacc gttctccatg ggacagccgg
    41521 gcagatgctc gccgccgagt ccaaaaccgc cgtgccgctc ccatctcccg gcaaaggcaa
    41581 aaatctcatc ttcctcaacc tgatcggcgg catgtcgcac atcgacacgc tcgatcccaa
    41641 agaagggccg actcaggggc cgggcaaacc catcaagacc aaggccggct ttcagctcgg
    41701 cgaaatcatg tccaaactcg ccgggcaggc ggacaagatc agcctcatcc gcagcatgac
    41761 ctccaagctc ggcacacaca gctccgcgca gtatttcatc cgcacgggtt acgagcagcg
    41821 caccaccatc atgcatccca cgctcggcgc atgggcgcag catttcaaag gccccagcca
    41881 tccgtccctg ccctccagcg tctgcgtgaa ccgcaatccc aacaacgcca acggcttctt
    41941 tcccgcctcg cattcaccgc tgcccgtcct cgatcccgat gtcggcctgc agaactccca
    42001 gcccgccgtt gaagcaggcc gcttcacgaa gcgcatggcc ttcctcgacc ggctggacgg
    42061 cgagttccgc caccgttttc aaactgcgtc cgtgaagagc tacaccgact tttacaagca
    42121 gaccgtcgcc ctcatgggca gtccagattt gaaagccttc agcatcgccg gcgagcctgc
    42181 cgccgtgcgg gagaagtatg gaaagacccg gtttggctcc ggctgcctgc tcgcgcgcca
    42241 tctcatcgag agcggcgtgc gctgcgtgga agtcgccagc gatggctggg acatgcacgg
    42301 caacatcgcc gaagggctgc actctcacgg cgcggagatg gaccaggccg tcgccgcgct
    42361 gctcgatgat ctcggccagc gcggcctgct ggagagcacc atcgtggcgc tctgcaccga
    42421 gtttggccgc ggtccggtgg tcaacgtcaa tggcgggcgc gatcactatc cgaaagtgtt
    42481 cagcaccatg ctcgccggag gcagcatcaa gcgcggctac gtccacggcg cgagcgacaa
    42541 gggcggctac gaagtcgccg accagcccgt gaccgtccag gacctcctca gcaccatcgg
    42601 ccatgcgatg ggcctccccg tggacgaagt gacgatgtcc cccagcaacc ggccgttcac
    42661 catcggggac aaaggcaagg tcatcacaga cctttttgcg tgatctgcct tcgtggcttc
    42721 gccattgtcc tatgcgcgca aggccgcctt ctcttcattg aagacatacc cgacagcacg
    42781 gcgcctcacg acttccgcct ccgcgacctc ctcaccctcg tcgccacaag cgaaatgatg
    42841 cggtagcggt gggtgcatcg cgcctgcgcc gtgacgcact cctttgagaa ccaacaaagg
    42901 atcgtgtgct ctgactcggc agcagccagg gccccgctgc tgaggtgttt atgcgtattt
    42961 tcccgtctga ccagactgtt ccgctcaaga cagcctgtga ttcggtcctc atactcacta
    43021 ctttaaccaa gccgctcatt gagcttcacg aatgatgaac cgccgacagg gtcttcgcgt
    43081 gcgttccgca tcgctgtttg cgggacattc gctcgttccg ccgacactgg catcagacgg
    43141 aaacagtcca cgaacatgag acatacaatc gcactcctcg ccacagctct gctactctgc
    43201 gcccaggctc ccgctgccac agcggacaaa cccaacatcc tcatcatcta tgccgatgac
    43261 ctcggctacg gcgatgtgca gtgcaacaac cccgagcgcg gcaagattcc cacgccgaac
    43321 atcgacaagc tcgcgtcgca gggaatgcgc ttcacggacg ggcactcatc gtcgggcgtc
    43381 tgctcgcctt cacgctacac actgctcacg gggcgctatc actggcgcac gagactccag
    43441 aggggcatcg tcggcgtgct gggtgagcca ctcatcgcgc cggaccgcat gaccctcggc
    43501 accctggcga aacagcaggg ctaccgcact gcatgcattg gcaagtggca cctcggctgg
    43561 aactggccgg tgacgaagga gcagcgccct atgcttttgc cagacaagaa aaccgccgtg
    43621 gccgccaccg agcagcaagt cgccctgtgg cgtgacattt tctccaagcc gatcgcgggt
    43681 ggtccgacga cgcgcggctt tgatctctac ttcggcacgg atgtgccaaa tcatcccccc
    43741 ttctgtttca tcgagaacga tcaaacggtc ggcattccca gcgagttcct ttccgccgcc
    43801 ttgttggtta acaaccaggc gagcgagcaa ggcccggcgc tcaaagactg gaagctcgaa
    43861 ccgatcctgc ccgcgctggg ggagcgcagc gcacagttca tcagcgagtc tgcgaaggag
    43921 cagcagccgt ttctgctcta cctgccgctg acctcgccgc acacgccgat ctcagtgaac
    43981 gctgagtgga agggcagaag cgggctcaat cccttcgctg attttgtgat ggagaccgat
    44041 gcggtcgtgg gccgtgtgct cgatgcgctg gagaaaagcg gcgcggcgga gaacacgctc
    44101 gtcattttca ccgccgacaa cggctgtgcg ccttacattg gtgtgaagga tctcgaagcg
    44161 aagggccact acccgagcgg cccgttgcgt ggctacaagg cggacgcgtg ggaaggcggg
    44221 caccgtgtgc cgttcatcgt gcgctggccc ggcacggtga agcagggcag cgtgtgccat
    44281 cagctcgtgt atcaggccga catcctccgc acactcgcag agctgtggag cgtgacactg
    44341 ccggacaatg cgggtgagga cagcttcaac ctgatgccac tgctaaaggg cgaagacaaa
    44401 cccatccgca agaacgcggt gagtgcctcc aaagatggtg tgcctgccgt gcgcagcggc
    44461 ccatggaaat acatccccgc tccaggctcc ggcggctggg gcagcggtgg tgatcaatcg
    44521 caacccgtgc agctttacaa cctcgccgat gacctcggtg aaacgaagaa cctcgccgca
    44581 gccatgccgg agaaggtggc tgagatgaaa gcgcttctcg aaaagctcat caccgatggc
    44641 cgcagcacgc ccggtgcgca gcagaagaac gatgtcgagg tcgtgcgcta tccacgcgag
    44701 aatacaccca agaagtcgaa gaagggctga cgccagcacc ctcgccatga aatccttcct
    44761 tctcctgctt tcctcattcg tcttttgtca gacgtcattt tcggcgctgc cgaacattct
    44821 cctcatcctc gctgacgacc ttggctacgg cgatgtgcgc tgctacaacg ccgactcaaa
    44881 ggtgccgacg ccgaacatcg acaagctcgc gagcgaaggc atgcgcttca cggatgcgca
    44941 cagtccggcc acggtctgca cgccgtcgcg ctacagcctc atgacggggc agatggcctt
    45001 tcgcgtgccg aatggcggca ccgtcttcac cggcgctggt gggccgtcat tgatcttcaa
    45061 aggtcgtctc acgctgcctg cgatgctgaa aaagcagggc tacaccaccg ccgcattcgg
    45121 caaatggcat gtcggcctca cgtttcgtga tgcggagggc aaaccgatcc acgaaggcgg
    45181 cctcgaagcc attcggcgca ttgacttttc gcgccgcatc gagggcgggc cgctcgatca
    45241 cggctttgac cgcttctttg gcactgcctg ctgtccgacg acggactggc tttacgcctt
    45301 catcgacggc gatcgcattc ccacgcctcc cacgagccct cttgaccgct ccacgctgcc
    45361 caaacatccg tatgccaatg attgtcgcgg cggactcatc gcgccggatt tcgacatgca
    45421 ggaggtggac atgctgtttc tgcaaaagag ccgcgacttc atcgcgcagc acgcacacaa
    45481 ctcaccaaag cagcctttct tcctctacca cgccacgcag gccgtgcatc tgccctcctt
    45541 cgccggaaaa gacttccgcg gcaaaacgca cgccggaccg catggcgact tcatcgccga
    45601 gttcgaccac atcgtcggcg aattgatgcg cgaactcgaa aaacacggcc tcgcacaaaa
    45661 cacactcgtt ttgctcagca gcgacaacgg ccccgaaacg acgagcgtcg ttcacatgcg
    45721 tgccgatcat tctcatgacg gcgcgaaacc ttggcgcggc gtgaagcgcg atgcctggga
    45781 aggcggccac cgcgtgccct tcatcgtgcg ctggcctgga aaggtcaaag cgggcaccac
    45841 cagcgatcaa accctctgcc tcaccgacat cctcgccacc gtcgcggcca tcaccggcac
    45901 cgaattgccc cacgatgccg ccgaagacag cttcaacctg ctccccgccc tcgaaggcac
    45961 cgccaccgct cccatccggc cttatcttct cacccaagcc tttgccggcc agcgcaccct
    46021 ttcgattcgt cgcggccagt ggaaatacat cgcccaccgc agctccggcg gaaacaacta
    46081 cgacaaaggt gagatgaagc ccttcgccct gcccgacacc gatcctgaaa cccccgccca
    46141 actctacgac ctcgccaccg atcccggcga gacgacgaat ctttactcca agcagccgga
    46201 gatcgtgaag gaactccaag ccttgctcga acaatccaaa accactggtc gcagccggcc
    46261 ctaaccgtcg gctgagcaat gtggcgcagc cgtcccggct gcctccgcgt ctgcatttgc
    46321 cccggcctcc ggaatcggct ataatcggcc aaaatcggct aaatatcagc caagaccacc
    46381 atgcttcccc ctgttttcac catcacgccc gagatgctgc ggctcatcgc cgagctggag
    46441 gatttcaaag ggcagtggcg catgatccgc agcctttcgc ccgaaaagct cacctcgctg
    46501 cgcaaggtcg ccaccatcga gagtgtcggt tcctccaccc gcattgaggg ttctcggctc
    46561 accgaccgcc aggtcgagga tttgctcggt cgcctgcaca tcaccgagtt caagacgcgc
    46621 gatgagcagg aagtcgcagg ctacgcctat gtcatagatc aggtgttcga tgccttcgat
    46681 cagatccccg tcacggaaaa catgatcggc ctgctccacc gggatctgct gcgctacagc
    46741 acgaaggacg aacgccaccg cggccactac aagaccctgc ccaaccacgt cgccgccttc
    46801 gatgctgatg gaaaggaaat cggcatcgtc ttccagacca ccgctccttt tgacacgccg
    46861 cgcgagatgg aggctctcat cgcgtggttc atcaaggccg accgcgagcg ctccctgcat
    46921 ccgctgctgc tcatcggcag cttcattgtc gtcttcctcg ccatccatcc gtttcaggat
    46981 ggcaatggcc gcctctcccg tgtgctaacc acactcatgc tcctgcgtgc cggatatgcc
    47041 tatgtgccct acagttcgct ggagagcgtc attgaggctt ccaaggaagg ctactaccgc
    47101 gccctccgcc gcacccagac cacgcttcag gcaaaaaagc ccgactggga agcctggctc
    47161 atgttcttcc tccgctccct cgtgaagcaa aaagacgcgc tcgcgaagaa gatcgccatc
    47221 gaggacaccc acgccaccga cctccacccg ctcgcgaaga atatcctcac ccacttcgcc
    47281 gaccacgaga cgctcaccct cagccatctc gtcaagctca ccaaaggcaa acccagcacg
    47341 ctcaagctgc gcttgaagga actcgtccag cacggccacc tcctgccgaa aggccagggg
    47401 cgcggggcgc attatgtgaa aaacactgct tctctataga actaaaccca aacaatctaa
    47461 tgcacatgcc aaaattttat agagaggcca ttcttgctac tcacagcatt agaggaacga
    47521 cacccgctac agaggtcggg cagttagaaa catggccaaa ggttgcacgg gctctcgatg
    47581 agctcgattc gtccggacgc tgccatgcca tttattccga ctggcttctc caattggatg
    47641 aaccgggcgg ggatgtgcag atccagatcc gcgatctctt gttgggacag ggagccttgg
    47701 ctgcaaatta ttttgtgact gtttacctgg tagcttgttg atatgtgttg ccgttggttg
    47761 cctcatttcg gaccgtgaag tatcgagctg cgcagcccga cgcaggagga accttgggcg
    47821 gctgcgaatt ttaaatggga cacgaactgt gttgccgttg gttgcctcat ttgattatga
    47881 atgtgttgcg ttaaatacgg ctacttttcg cctggcagct atttttttgc aattattccc
    47941 cgctttccta ctcagattgt aaattcattt ttttgaagac ctccatatag tcataattgt
    48001 gacggaatat gtgcccatct ttgagtgctc cgaatttctc tttttgggag tgtaattcag
    48061 gaaatgttcg atttatgtat aaatctccgt tttttaatat ttccaaattt cccacatgtg
    48121 atatgttcat aattaatact ttatcatcat ttccaataaa caataaggtg ttgctgcttt
    48181 cggatttcca tactccagta ttatctgaac tatccccatc aatgaaatta gactttagaa
    48241 tcatcctgat gtagtcctga actgagtcaa catcaatttt gtaatttgga tttcgagtgt
    48301 ctgcacattg tataattagc agcattgtgc atgcggttat tataaatttc agcttgctca
    48361 tcatattgtt aattatgtat tttatacttt ggaccattag tctgacccca aggatataat
    48421 ccactaggtt tcaagttgag cagattgctg tcatagctag cttgtatcgc ctcaatttgc
    48481 attggatgca actctgagcc acctttataa cccataaggt ttttaggtaa attaagcttg
    48541 attgggttcc ctttatcttt gccaacagtc ttcccttttg atccatctac tactacgacg
    48601 ttcatttcta tcttggtatg atcatattga ttgccttgtt tgttagtctc tttttttgtt
    48661 gtagctttga catttaagcc ctttggatca aatccgctcc acgggttctg cttcacatag
    48721 gcatagaggt tcgggccgtc cacaaagccc gccgggtcgc ggctcagcca ggtcatcgtt
    48781 tccagat
//
LOCUS       DAOMYJ010000008        48376 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Prosthecobacter sp. isolate AMR_MDS_5629
            NODE_284_length_48376_cov_23.653567, whole genome shotgun sequence.
ACCESSION   DAOMYJ010000008 DAOMYJ010000000
VERSION     DAOMYJ010000008.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867850
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prosthecobacter sp. (wastewater metagenome)
  ORGANISM  Prosthecobacter sp.
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Verrucomicrobiaceae; Prosthecobacter.
REFERENCE   1  (bases 1 to 48376)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 48376)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.30
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:34:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,742
            CDSs (total)                      :: 4,684
            Genes (coding)                    :: 4,676
            CDSs (with protein)               :: 4,676
            Genes (RNA)                       :: 58
            tRNAs                             :: 55
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..48376
                     /organism="Prosthecobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_284_length_48376_cov_23.653567"
                     /isolate="AMR_MDS_5629"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1965333"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..330
                     /gene="accB"
                     /locus_tag="PLP58_01575"
     CDS             <1..330
                     /gene="accB"
                     /locus_tag="PLP58_01575"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HRJ07242.1"
                     /translation="AAYPPQVAQVPPPAAPPQEAARSAPADTAPAGPTINSPMVGTFY
                     RSAAPGESSFINIGDPVDENTTVCIIEAMKVMNEIKAEMRGTVARVLVEDGKPVQYGQ
                     PLFELKA"
     gene            402..1763
                     /gene="accC"
                     /locus_tag="PLP58_01580"
     CDS             402..1763
                     /gene="accC"
                     /locus_tag="PLP58_01580"
                     /EC_number="6.3.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009959148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_function: GO:0004075 - biotin carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HRJ07243.1"
                     /translation="MFRKVLVANRGEIAVRIIRACKELDVKTVAVYSEADVDSMHVHL
                     ADEAICIGPGPSTESYLKISRIISAAEIANVDAIHPGYGFLSEKEEFAEVCDKCKIKF
                     IGPSARVISMMGDKNTARATARAAGVPVTPGSDGIIHNEEQAREWAGKIGYPVIIKAT
                     AGGGGRGMRPVLNEATLVSSFQAASLEALKCFGDGSMYMEKLVEKPHHIEFQVVADSH
                     GNVVHLGERDCSMQRRNQKIIEECPSPKISDELRKKMGDATVKLCKEIGYENCGTIEF
                     LVDNNRKDFYFMEMNTRIQVEHPITEEVYGCDLIKEQIRIAAGLPLSEHVLTAGARGH
                     SIECRINAEDPARNFAPSPGRINLWYTSGGRGVRVDTHVYSGYEVPPYYDSMIAKLIV
                     TGATRDIAIRRMRRALGEFTVEGIKTTIPLQSKILTTSDFQNGNYDITWVENFLRQEG
                     MKG"
     gene            complement(1835..7012)
                     /locus_tag="PLP58_01585"
     CDS             complement(1835..7012)
                     /locus_tag="PLP58_01585"
                     /inference="COORDINATES: protein motif:HMM:TIGR04534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ07244.1"
                     /translation="MKKIQKISAGILLISSSAWGQGTVLLKDINPTGLSAPTEAASLA
                     KVNPGSGEIMIISVQDSLHGSEVWKSDGTPGGTAIIKDIVPGNGGSAPVNLTPAGTQM
                     FFSAANDASPPKRNLWASNGMAAGTNLVKDLGFFGSDGPEKLFNFNGTLYFQGFSGTA
                     GLELWRSNGTDAGTVLVSDIRPGGGDSAIDHFYVSGTGPSAILYFTATDGQNDVGLYR
                     LNPAGTGVERLGVSEPLFQQFGRIAPQYVNMNGLTYFSAFSGNGVELWRTDGTAGGTQ
                     EVFDLFPGPSSSEPSHMTVMTITGLQAGTYLYYSATDGAISGVELWRSDGNPLNTPEQ
                     LKDIFVGGDSSNPSQLMVVGSNLFFTADDGSGVELHATNGRTGNENSARVADIVAGTG
                     GSNPGNFVNFNNTTLLFTAVDSSNNLGLYTCASGASLPNAVTRIKSLGIESTASHMTR
                     LGSKVFFLLNNSQLWETDLTNSPAGNSNGTKMVFNFRSGSRGSSSGEFAVAGTDVYFS
                     ADDGVTGRELWKSDGSDAGTMRLADIFPGDDGSFPPTPNSSDPLWITPCGPNVFFSAE
                     NANANRELWIYDPTANPAVFQVKEINPTGPSNPENLFDLNGLLLFTAEDGSGFGTTGR
                     ELWRSNGTEAGTVQVADLVTGPEGSFPRQFTPFKNEVYFFAGRDGVGRELMKINGTGT
                     TVTLVKDIAPGGLSSINEENESMAVMGSGASERLYFSARNESQGGRELWTSNGTDAGT
                     VRVKDINPTGDSLPRQITTVDKVVFFTATDGVNGTELWRSNGTEAGTVLVRDINAGAP
                     GSDPQELTASGKLVFFTADNGLDGRELWTSNGTLAGTVMLTDFRNGGNRASSMGITGL
                     KDVDGVLCFSADDGVNGRELWISDGTVAGTRMINDISGIPTASSPGGFTPFRGQLLYT
                     AADTEDGDEPRIAFIGSDIRVEQPAANQLAQNGPAVDFGVADVAAKESTMLIFTIRNA
                     GLNTLRDIKPVLGGINAGEFSLLSTPKPASSLAQNATTTFGVKFTPKEGGPRVAQVSI
                     ISNDGDENPFIVNVTGTGVKDPTITDHPDSLMRNVGEAAAFDAMAASSSVALTNADYQ
                     WRRNNGAIKGALGTGFNIPAVTLKEAGAYTVAVKVGKILGISNPAQLGVVQANTPPAV
                     LVVGKTKSATMKVSAAGNGLTYQWFLGAVPVANTADGRVKGAQTPTLVIGNLDEPDSG
                     LYSCQVTGPGGMRAGGHTELKVFTEKPVLDDPQNMPDGIIGGSYGFPLEGFDIMVDAA
                     PEKAPTTYTAANLPAGLKVNPKTGKITGRPTKSFPAGRDVTLGAANTQKADPVVETII
                     IKDFPANLAGTYSGTVDPDTALNDGLGGRIDVTISATGALTGTLTLGAERIPLKGGIE
                     VDAAEVLPPNYTVTLKRKNGTNVTLAFDMIMDNIVTTQRLLTGNANGIPVNGWRHAVP
                     AEAAAFQGYYTFGLELGDPGHPGQEHIPQGWSYGAFTVDKNGKWKIAGRTADGETITC
                     AASVGFAGQMVVFQTLYKPLAGSLWGSLVIDPVDTNNAEDNSLSGDVNWVRPANTNTK
                     TRAYQAGFGLPGTPEASPVPLEATGGIYVDPTKTKVLIMGLASPGTQTNLNLDFEQGD
                     LLTSTQDPDQILGILDGNKIANPKPAGPTSTLIKKLAAKTGLFDGTFRLTDPDQRTGA
                     TGNLIRDAVFHGILIRDDVEMKTYGVGHFLLEKRPQDGEVPKKTVPVLGGWFELREN"
     gene            7192..8298
                     /gene="leuB"
                     /locus_tag="PLP58_01590"
     CDS             7192..8298
                     /gene="leuB"
                     /locus_tag="PLP58_01590"
                     /EC_number="1.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009962135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003862 - 3-isopropylmalate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydrogenase"
                     /protein_id="HRJ07245.1"
                     /translation="MSRTHKLAVLPGDGIGPEVMAQALLVLDSIAAKSGIHFEYNPQL
                     VGGAAIDAAGKALPAETIAACEASDAILFGSVGGPKWEHLPPNEQPERGALLPLRKHF
                     GLFANLRPGICYPALTASSPVRPDLVEGGFDVLCVRELTGGLYFGQPKSRDTLPDGDI
                     QVIDTMVYKKSEIVRIAHVAFKAAQLRGKHVTSVDKANVLTNSVLWRETMIEVAKEYP
                     DVTLAHLYVDNAAMQLIKAPRSFDVLVTENLFGDILSDEMAMIAGSLGMLASASLGQP
                     KGDGLYFGLFEPSGGTAPDIAGKGIANPIAQILSAALLLRFSLGLENEALAIENAVKN
                     VIGDGLRTGDIFTSAPGTRLVNTAEMGAAIVAAL"
     gene            8367..9068
                     /gene="queC"
                     /locus_tag="PLP58_01595"
     CDS             8367..9068
                     /gene="queC"
                     /locus_tag="PLP58_01595"
                     /EC_number="6.3.4.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009964250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine synthase QueC"
                     /protein_id="HRJ07246.1"
                     /translation="MTRTLVLLSGGMDSTAALHWARREHEVAGAVSFDYGSKHNAREI
                     ELARWQCEKLGVPHDVLALPFINEHFSSDLLLSGGNIPEGHYADENMRRTVVPFRNGI
                     MLAIACGLAESRGARALVIAAHAGDHAIYPDCREEFMQAIAAAMRAGTYAGIELLRPF
                     IHLDKAAIARLGHDLGVDFGMTWSCYKGGGIHCGVCGTCVERREAFLKAGLPDPTRYL
                     DEGALPAPAVLPPSS"
     gene            9238..10239
                     /locus_tag="PLP58_01600"
     CDS             9238..10239
                     /locus_tag="PLP58_01600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009962176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HRJ07247.1"
                     /translation="MSDTAAAAALVDGFQKLKAALSRRIVGQEAVIEQVFIAIAAGGH
                     SLLEGVPGLAKTLLVKSLADAMHLSFRRIQFTPDLMPADITGTEIIQEDAETGRRKLV
                     FQPGPIFSQIILADEINRTPPKTQAALLEAMQEKHVTVGQETFDLPKPFFVLATQNPI
                     EQEGTYPLPEAQKDRFLFLIKVDYPSREEEREVIARTTGGAQEEIKAVVTAEELQAAQ
                     ALARKVPVPDHVTDFVLDLVRATRPNEPDAMPYVKEMLSWGAGPRASQMLVLAGKVRA
                     LLQGRTHVTLDDIEALALPALRHRLVPTFHAEAEGVTVDMIVAELIKRVKKPEARVL"
     gene            10294..11187
                     /locus_tag="PLP58_01605"
     CDS             10294..11187
                     /locus_tag="PLP58_01605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009962177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HRJ07248.1"
                     /translation="MASLSDILHAEDITSLQNLQLFARTVVEGFTTGHHASPHKGFSV
                     EFRQHRPYVRGDDIRRLDWKIFGRADRFYIREYDEETNLRATLVLDASGSMGYRGQKG
                     VLKFDYARKLAAAMAYLLMSQQDAVGLVTFDSRVREFIPNRTRITHLHHLLETLVKTE
                     PGEETGLAPVLESLAQRLKRRGLVILISDFFDDADAILKAIGVLRKKGHEIIAMQLWD
                     RDEIEFPFGQWSRFENLEQDEDFLMLDPAAVRLRYLEVRRAFEEQLKEGFRKHQVDHL
                     SLPTDESHAAALRAYLALRMR"
     gene            11246..12031
                     /locus_tag="PLP58_01610"
     CDS             11246..12031
                     /locus_tag="PLP58_01610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009960051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="creatininase family protein"
                     /protein_id="HRJ07249.1"
                     /translation="MSQPPRPWIIAETNWKQVKAGRYEVAVLPWGATEAHNWHLPYAT
                     DSLQNEAICAGAGRIAWEAGARVAILPNIPFGVQTGQLDIPFCINMNPSTQAAVLEDV
                     ITSLEGVGVPKLVVFNGHGGNDFRQILRELQARHPRVFLSLVNWFQIPGAQDIFTILG
                     DHADERETSLMLHLHPTLVLSKENWGPGTENPWKLKAMRERWAWAQRAWTQATNDTGV
                     GDPQHSTAEKGRLYFELLAGKFAAHLVELAAADTGALYDPTPV"
     gene            12166..14313
                     /locus_tag="PLP58_01615"
     CDS             12166..14313
                     /locus_tag="PLP58_01615"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HRJ07250.1"
                     /translation="MLDRILSCLFLLPILAGAAELAASAPAAEDEGREPASANTQFPP
                     VVVTATRDGIEALKAPNQVRTLDSREMLERQARTVTEALRETPGVNVQKTSNAQGSPF
                     IRGFTGFRNLALIDGIRFNNSTFREGPNQYWNTIDSYAIDHIELVPGQGSVLYGSDSI
                     GGTINLFTKGPGFRDEESGFFFHGLSSYRASTAERSHMGRQELQFGQGGRWGLHLGAS
                     LKSFGDVRGAGVGIQPHTGYDEWAYDMRLDLALDENWTLTGVHQQLRQNDAWRTHQTT
                     SGIQWRGTTTGTDLKRAYDQERTLSYLRLAGKNLDSFVESAAFTVSLQTADEYEHRIR
                     RPADNRIDFTQVEVTTLGLDLQLVSVTPVGRLTYGVDYYRDWVSSGSQRYRLNGTFHS
                     RGIQGPVGDDATYDLLGLYLQDQFDLGSRFHVFLGGRLTHAAAEVGRFNDPATARVVD
                     SYSDDWQNFSGSLRLVLDLDEEDRFKLFGGVSQGFRAPNLSDLSRLDIARSGEREIPS
                     TGLDPEKFVNFEIGLKAETESISAGLSYFHTLIDDMIVRRPVGGGDVLKDNSGTGFVQ
                     GIEVNGEWRLDPQWTLFGHLTWTEGRVDQYPRTNSTHQRAEPLSRVVPLMGRAGVRWQ
                     SSGRRLWTELVCIAHSRFERMNTGDRRDTQRIPPEGNPGFENLILRGGWLVTNNLELN
                     VALENLLDQEYRHTGSGSNEPGFGAVLGATFRF"
     gene            14408..15034
                     /locus_tag="PLP58_01620"
     CDS             14408..15034
                     /locus_tag="PLP58_01620"
                     /inference="COORDINATES: protein motif:HMM:NF012774.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thermonuclease family protein"
                     /protein_id="HRJ07251.1"
                     /translation="MSRRPASRLPRPFVILLVLAFAAWHYWQEAQPPPDAPPRTGPAL
                     SSHEALHGARLLDHRDNDGDSFQILHNGQAHHFRLYFADSPEKRRHQHNGDRLRDQGR
                     YFGGLEEARTIALGLEAKKLAENLLRSRPFTIHTRWHQVYDSGRYYAFVIFDDGEDLG
                     EKLVRAGLARIHTTGAPHPDGRSAQAFEKHLRRLEQEARSEARGGWAR"
     gene            complement(15123..15797)
                     /locus_tag="PLP58_01625"
     CDS             complement(15123..15797)
                     /locus_tag="PLP58_01625"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HRJ07252.1"
                     /translation="MRRLRQMLTFLALSASAGLLWHETRALRVASRPSAASFDPALHR
                     LGAAETAALPLASRFDHPLGSEHGALTYNARPFRVARHLGDDLNGIGGGNSDLGDPVH
                     AIADGCVVYAGSPGPGWGKMLILAHRVPADDPVEPPRVIQSVYAHLQDILAEPGARVR
                     RGQRIATVGSAEGQYLAHLHFEIREGPYINPGAGYADTPLNRVSPEAFIFQHRGAPAH
                     QLNPPP"
     gene            complement(15817..16818)
                     /locus_tag="PLP58_01630"
     CDS             complement(15817..16818)
                     /locus_tag="PLP58_01630"
                     /inference="COORDINATES: protein motif:HMM:NF013856.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HRJ07253.1"
                     /translation="MHTFLAADDAIEPLTGELGGADMEGVWLRSENLPAHLAFARQTL
                     PRARRVEAASVNAWADQILQSIVGVLPEEGTWRLHLWPAYGEGRAGEHRCRLIRSALA
                     EKLKKQRRALMRCQEESQEPFTRSTSLVQAVLTSPGSGLLSLSAAPEPHELRSIISAY
                     PAGHITPAEDKTAPSRAFAKVIEAEMRLGRRIMKNETCVDLGASPGSWSHVALLRGAH
                     VTAVDRSPLRADLMRHPRLHFHQGDAFKFRPPEPVDWLLCDIIAAPQRSMDLLLDWLR
                     ARLMRAFIVTIKFKGTDEYGQLDTLKQNAPPLCADFRLTRLCANKNEVCAFGIAARE"
     gene            complement(16823..17806)
                     /locus_tag="PLP58_01635"
     CDS             complement(16823..17806)
                     /locus_tag="PLP58_01635"
                     /inference="COORDINATES: protein motif:HMM:NF013775.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HRJ07254.1"
                     /translation="MPPEHEALLILTRQRFPALADCPCEVEPILKGGSDRRFYRFTWA
                     GGAQPPMVLMIYTQARRDNARFVPATRRLAALGAHVPAILGFDEQRLFVWLEDLGRDD
                     LHSHRDDPWEARRPLYLSALAEAAKIHSVGEDDLTSADIAEMEPGFDEGLYEWEQNYF
                     LEHFVRGYLRRETDTSEVQPAHEALSALRSGLATLPRCLVHRDFQSQNILLRDGAAWL
                     VDYQGLRPGLAGYDLASLLFDPYVTLGRAERHELLEHYAGLRGLALPDLRGVVYQCAA
                     QRLMQALGAYANLSRNQGKTHFEQHIPSGVRNLAGVCDEIPALSPLRGFFC"
     gene            17958..18680
                     /locus_tag="PLP58_01640"
     CDS             17958..18680
                     /locus_tag="PLP58_01640"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HRJ07255.1"
                     /translation="MQFVFPTHLKLGEEFSEGFHQELLRGLTHKLNNLLAVIQGFSSL
                     ILMNEDLDPAVAENMQHIREASTGVTALSERIRSAGGCAKLSPQPLSLNDYLGVIEGA
                     LTEPFRANGVQLEADLQPGLPPIHVDPTKFKDILVEVLKNAAEAAGKAGGRAILKITG
                     PGGLTPADQRRVDILVSNTGSQIPPEKLAEVFRPFHGTKNSNHLGLGLTIAAMLCHQM
                     GLQIGIASENNTTTVWISCPTA"
     gene            complement(18684..20102)
                     /locus_tag="PLP58_01645"
     CDS             complement(18684..20102)
                     /locus_tag="PLP58_01645"
                     /inference="COORDINATES: protein motif:HMM:NF022466.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2851 family protein"
                     /protein_id="HRJ07256.1"
                     /translation="MSLTPLPHAERYARFRDSVFAGLEEGPAACLSGLEASMSEMDVQ
                     SLWFGGAFGAEFTDPEGRRVRMADFGEWNSGAGPDFTNTAVEVDGQLLRGDIELDMDA
                     RDWERHGHGANPEFGRVVLHLFVHAPKERFYTRTHEHRAVAQARLPMENLAGSALPNR
                     GLAAARLGRCHTPLAEMGAARVASLLEAAAQHRLENKSRRIHRCAEAQGRAQTIYQEL
                     ARTLGYQKNPQPFVLLAQRLPLKRMLKLPPMQREALLFGVAGFMEAVRHEETAGRSRE
                     YLSGLWSEWWKHYDEASRWLQPRHLPRWRLSATRPGNHPQRRLGALAAMLASWERVAG
                     PLMDAGRWSRDAWRETMTALRHDHWSAHYTLTSAPAAAPLALVGETRVQEMLANVVYP
                     LLIPERTRLWAEYLDLPALLENSRLRRAVARLFGNSTLKKDFGKKLHHQQGLLQVYED
                     FCLEDDSGCAGCPFPERLKEWA"
     gene            20240..21250
                     /locus_tag="PLP58_01650"
     CDS             20240..21250
                     /locus_tag="PLP58_01650"
                     /inference="COORDINATES: protein motif:HMM:NF016629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rpn family recombination-promoting
                     nuclease/putative transposase"
                     /protein_id="HRJ07257.1"
                     /translation="MPAKIPAAESPAPTHDDLIKKLLHQPRLLRDFIRAFLPELAKFA
                     DLDHIEYLDKEHPRGSKRPRRLGDVLFKTLWKKCETAFLIHVESQNKPHPAALERMGE
                     YALRDGIRYRLPVMPVLLLTYPKPETPQPGELRWDFGSLAAIHVKCPVLHFRRMNPLP
                     HLRSKNVAALAMTALMKLEADQQVDAIVSTMAEALRQEFEAEDLDAALDFVNHYLPLA
                     EEQVLQLDRKVDSLARKEPKLKPMPKLINPFVEIGRLRGRKEGRVEGRVEGRVEGLFE
                     GRSGLALRLLARKFPGIARRAGPLVKRLDAEQLLAFGEELLFMQTPAECMDWLKRGTG
                     KY"
     gene            complement(21306..21965)
                     /locus_tag="PLP58_01655"
     CDS             complement(21306..21965)
                     /locus_tag="PLP58_01655"
                     /inference="COORDINATES: protein motif:HMM:NF024811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HRJ07258.1"
                     /translation="MSSPIRAVIFDFDGLIVDTESTGYRTWKEIFARHGHELPVERYA
                     QVVGTDFNTSYDPRRDLEELTGLVFDWDEIEEGRRTRETELRQTLQTLPGVVDRLHEA
                     RALGMTTAIASSSPRWWIDSWMDQLGLGAHFDHISTVDDTGRVKPDPSLFLHAAEKLG
                     VSPEEAVIFEDSLNGLRAALAAGIRCVVAPGPMTRHLDFTGALRRVESLAEVTLRELG
                     Q"
     gene            22076..22636
                     /locus_tag="PLP58_01660"
     CDS             22076..22636
                     /locus_tag="PLP58_01660"
                     /inference="COORDINATES: protein motif:HMM:NF020906.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1990 domain-containing protein"
                     /protein_id="HRJ07259.1"
                     /translation="MFHLSKPAPDDLAARLSEWRSEPLSYKHQGGIGIPPETGFIVDA
                     HEVLLGRGESVFQSAGAALDEWKMFPAWAGVARVRASGQEPGEIVAMIARICGLWWVN
                     PCRILRRCDGPRSHGFVYGTLPEHAECGEEQFVIEWRADDSVWYVIRAFSRPRHWMAW
                     AAFPLARWWQCRFVRDSQARMKGAVS"
     gene            22975..23670
                     /locus_tag="PLP58_01665"
     CDS             22975..23670
                     /locus_tag="PLP58_01665"
                     /inference="COORDINATES: protein motif:HMM:NF025523.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YndJ family transporter"
                     /protein_id="HRJ07260.1"
                     /translation="MPAGFFLALPWLAVRSWSGVVVLRRWRHEGFGGLGHACVALAQV
                     FPAIGAAWLVAHRANWTPWNFDPLIVLLTAAHFHHAGFTLPLIAGLNARASPGRLTSA
                     SCAAVLAGVPLVAAGITCAHFGMLPLLEPLGVMVLVAGAMGVAASQLLTGVASRGGMR
                     WRTRAAFLFSGACLIVAMILALSFGLRYLFPRLALTMPQMWMIHGSLNTFGFGLCGIL
                     AWRSLAGRGQSAG"
     gene            complement(23655..25730)
                     /locus_tag="PLP58_01670"
     CDS             complement(23655..25730)
                     /locus_tag="PLP58_01670"
                     /inference="COORDINATES: protein motif:HMM:NF012321.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BatA domain-containing protein"
                     /protein_id="HRJ07261.1"
                     /translation="MSWLFPLYLLGAGAIIAPILMHLRRRPPQDRVEFSSLMFLQART
                     PVPVSKRRIEHWLLLLLRCLALILLALMFSRPWLQTDARAGADGGRAVFMLVDRSASM
                     RRGDLMKQALGRAREVIQKTTPADRMALAAFDDRLHPLWTFGEDAESGPARSGALLQK
                     LGEIQPGWQATDLGRALVDALAQISSASGIGGMERVIVVLTDFQEGARLDALRGIAWP
                     EETRVQALRVETRDEGNMTLSLAAADTGNVEDRSAKTGEAETLRVRLSNSRDSRVSDF
                     TLRWEKSRGEAMTGYLPAGAARILSVPLPTQAAASADTLLLSGDSWDFDNRIFLAPAR
                     PRQVGVAWLGAKAAADEAASPLFYLKRALQPTTSLEPQVADAAELPRCQMAVLQGAPP
                     EGFAEWIRAGGLAVWLLEPGTSAQDVESLAGVNGVALKEAPVDNYRMLGEVKAEHPLL
                     RPFADPRLRDFTKLRFWKHRQADLTGTAAEKIEIIARFDNGAPALMALQMEKGTLLML
                     ASGWHPADSQLALSTKFVPLIFGWLEAAGFSHAAQSGMLVGAALPVTAGDALVLTPEG
                     RETTVKAGMPYVTESVGIYQVKTGAETRLHAVNLPPEEGRVTAMDAERLKDFGIRLHE
                     MSGTQGEIAQNERVQERLAAGEAEKQQRAWLWFLAALLAVLGAETLLAWRAGRGRVEE
                     AAPAPQPAL"
     gene            25979..29941
                     /locus_tag="PLP58_01675"
     CDS             25979..29941
                     /locus_tag="PLP58_01675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ07262.1"
                     /translation="MKLAFQIILPGLLSLSAAAQDVRWTGALSEFWDNSRNWARATPP
                     NSDGDVVFPANALRKACQNNLPAGTALSKLVFDAGGYVIEGNPLRIKSGGIILADGTA
                     AERVVSLRLPLTLGASQTFSIGGLAGSAGGLLLHLKGAVSMGDFPLQIEGGDAVLDAP
                     VTANMRGGTALKFTGIGVKTISQGVTITAPRIEVSGLAGHVRLLGEVVADTMLVTALL
                     PQTPGKGGVLSGTGRVEADCRVLRYGVLSPGVEGFGRLRMDKNLVLGGDSLVEIELGN
                     PSSAGKTHDQVRVQGAVEIQTGASLLIKPGAGFAPVAGQAYTVLEKTGAGPVTGRFQN
                     LPQGATIRVGEATMLVSYTGGDGNDVTVTVVDLPPAISAIPAQTVHEDGRVTVAFTVS
                     DPNTDLSALRVSLRGGQVTASFSGAGAARTATIRPQPDFFGQTTVTLEVSDATVSTRR
                     SFELEVLPVNDTPSFTAAPVIEFVRGALQSRAAASNISAGSGEEQALAFEVLSVSNPQ
                     LFRVLPALTPDGMLTCGPVETGESLVRVRLLDDGGNDHGGIHASPPQTILLRVGADKE
                     SPIAPPVDVTWTGLAGSQWNNGRNWSTLLVPQHAATAGFAADLPARRSKSVDISGGAQ
                     CGQLRFSGGGYRLHGGDGDFILAAEARLTAGVDTRFDALLKGRESLSLIAGQGARLEL
                     KGGLECPFRGFALPIPPVRPNKLFMNLGFGSVMEINGGRLADVDILMGGKGRIVMEGD
                     VFHMGETNLFNGGFHVLEVNGSFGTRLTHVERDLNSVVFQSTATLRGTGTVLGRLSAG
                     GLIQPGRTGGVEGDAAGQIGRLTVHIATVVDNGAENLGVLELDIGSAAGPGVTYDQLI
                     VEKRLVLDNARLRLRPAQGFAPRVGDVFTLVNVAGPVTGARTGFFSGLPEGATLRVGN
                     ADLRVSYTGGDGNDITATVTALAAGASGFASASTLTNAATFTPGSYCGLAVADQGVLP
                     GHENAGLLSLTVTQTGTFSGKLLMGGASHKFSGAFKSDGSALFGKAGGLLTLPRKNLA
                     PLELSLYADSSEGAPSVTALILEDGRPFASARADRALYDSVKSPAPAWMTDAKSGKGK
                     FTAAFPALAAPNRGLEAASFPQGDGWALMSLARDGRVTMTGQLADGAQITLSGLITTG
                     GSLSIHVPLYSGKGSLSGMARFEQQPLTDAAGVDFFWFRPAQTPRAAAPYAAGWPQGI
                     HVDFDACRLVEKAAPYLAGLAAPGPAGNVLITVDGLAQKAASLEPANKIVLVNRPDPD
                     KLASAITTSSGKWSGSFIHPADMKKKAFSGVVLQKSGRATGFYLAPQGSGLAGFSVVP
                     "
     gene            30140..31579
                     /locus_tag="PLP58_01680"
     CDS             30140..31579
                     /locus_tag="PLP58_01680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009958772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1501 domain-containing protein"
                     /protein_id="HRJ07263.1"
                     /translation="MSHHAPNLDHHFLSRRQLLSRVGMGFGAVALGEMLGAGKAHGAL
                     DQRSPFAARRPHFEPKAKRVIHFFMNGGPSQVDTFDPKPMLDKYDGKPLPNLLKTERP
                     TGAGMRSPFQFKKYGQCGLEVSDIFSRTAAFADDLCVIRSMQADVPNHEPSLGLMNCG
                     ESRLHRPSMGSWITYGLGSENQNLPGYISMCPKGMPTRRTKNWQSAFLPSIYQGTYIN
                     TEDTEVDKLVEFIKNSSLDLKQQRRQLDLLHALNEQHLRQRDNDQQLDARIQSYELAY
                     RMQMEASDAFDIGKEPEYVRQAYGENDFGRQTLIARRLLERGVRFIQLWTGAGQPWDS
                     HDEILDHQRLAKDVDQAIAAFMHDMKQRGLFDDTLMLWGGEFGRTPSVELPTPGANAG
                     KQRGRDHNHYGFTCWMAGGGARGGYTHGATDEFGFQAVENPVHVHDFHATLLRLLGFD
                     HERFTYRYAGLDFKLTGVNDCKVVRELLA"
     gene            complement(31741..33855)
                     /gene="glgX"
                     /locus_tag="PLP58_01685"
     CDS             complement(31741..33855)
                     /gene="glgX"
                     /locus_tag="PLP58_01685"
                     /inference="COORDINATES: protein motif:HMM:TIGR02100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004133 - glycogen debranching enzyme
                     activity [Evidence IEA];
                     GO_process: GO:0005980 - glycogen catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen debranching protein GlgX"
                     /protein_id="HRJ07264.1"
                     /translation="MLFSQEKTIPPDPLPPGVTLTKNGAHFTVFSRHATAVELCLYSP
                     FAPDTETGRHSMTRGERDLWSVFVKGARPGTLYGYRAHGPWMPDKALRYNPNKLLLDP
                     YALAIAGVPDGRTGMLNTHGPGTEPGSVDNGPTALKSSVIAGDFDWRGDERPRIPWAD
                     TVLYELHVKGFTRTHPRVPAGLRGTYAGLARPEVIRYLKDLGVTSLQLLPVHQHLDDQ
                     FLLEKGLTNYWGYNTIGFLAPHNEYAAARDPEGCVREFKQMVRALHDAGLEVILDVVY
                     NHTAEGDERGPTLMFRGLDDRLYYRHSFHEHGASYINITGCGNAVNSASPPALRLILD
                     SLRYWVTEMHVDGFRFDLAVTVARDGRDAFDSNSQFLAAVAQDPVLSQVKLIAEPWDI
                     MRMDSYQVGAFPEPWRELNGKYRDSVRRFWTNSPGSTASFAKRFCGSQDVYGWSQRPA
                     LSSVNFITSHDGFTLADLVSYSRKHNEANGEDNRDGDSSNHSTNCGVEGPTDDPAILQ
                     RRARLKRSLLATLFTSVGVPFLTAGDERGRTQGGNNNAYCHDSDLTWLDWSSCDEALL
                     AFTRRIIALRRATPALRRATFFDGQTDPATGLADILWLEGNGTLLCHEEWHEPGRYHF
                     GALLGSQPPLLLLFNRGNHDQDFLLPGDKATLWEILFDTTVDHPPRHPARHHGARIFA
                     LPAHSYICMRLLNGLAIVSPTC"
     gene            33981..35207
                     /gene="trpB"
                     /locus_tag="PLP58_01690"
     CDS             33981..35207
                     /gene="trpB"
                     /locus_tag="PLP58_01690"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009958357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
                     /protein_id="HRJ07265.1"
                     /translation="MTAATILPLPVLPDAHGHFGKYGGMFVPETLMSALTELSDHYEK
                     AKADPAFQKELDGLLHEYVGRPTPLYFAERWTEKLGGARIYLKREDLLHTGAHKINNA
                     LGQILLAKRLGKKRIIAETGAGQHGVATATVCARFGFDCTIYMGKVDMERQALNVARM
                     RFLGARVVAVTAGQATLKEAVNEAMRDWVTNVRTTHYILGSALGSHPFPMMVRDFHRV
                     IGVEARRQILEREGRLPDLLVACVGGGSNSIGLFHPFLNDEGVRMTGVEAGGDGIIPE
                     RHAARFQGGRLGVLQGTKTWLLANADGQIELTHSVSAGLDYAAIGPEHAWLHDQGRVT
                     YNYATDDEALAAFQELSAVEGIIPALESSHAAAEVRKVAPTMRRDQIILVNLSGRGDK
                     DVAQAARMIFGEELKF"
     gene            35261..39226
                     /locus_tag="PLP58_01695"
     CDS             35261..39226
                     /locus_tag="PLP58_01695"
                     /inference="COORDINATES: protein motif:HMM:NF024777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HRJ07266.1"
                     /translation="MLRPILLSLLATAAHAQTAADLLTQSGVKGGLVVHVGASDAKIT
                     AGLRANDSFQVRALTRDAATLEKLRAGIGADYGVVSADLLSTPHLPFIENLVNLLVVE
                     DAQVTREEIDRVLVPNGVALIKKDGGWEKIVKPRPAEMDDWTHYAYDSKGNLTSKDML
                     VGPPKRMQWVGNPRWSRHHDRMSSVSAKVSEGGRIFYIMDEGSRISILVPPKWMLIAR
                     DAFNGTVLWKKEIPEWSTHLWPLKSGPTQLTRRLVAVDGKVYVTLGITAAITCLDGAT
                     GEVVREYPETKGTEEILVRNGTLYALVNPDADRLNKEFAVAAQSDQKRVETEFNWDGK
                     PRILHAIAADSGKVLWKKEDRIAPITLSLDDKRAVYYNGDGLACLDIKTGETKFADTP
                     TQRRALYEFNFAPRVLLHDDVILYAGGDGSQKAVNADTGKDLWVAPHEKSGYRSPEDL
                     IVAQGLVWNAGTLSGNQTGEYTGRDVLTGEVKKQFYPDVPEGTYWFHHRCYQAKATEK
                     FIIPSRTGIEYVDMEKEHWDLHHWVRGACLYGVLPANGLTYAGPHNCACYPEAKLDGM
                     AVMASTARYPLPAHTPDDQRLSKGPAFGQAIEEKDAAAGDWPTYRADNARSGHAKADL
                     RKDMSLAWEVKLTPPLSTTTIAAGLTFVSEVDKHTLHAIETATGKTAWRFVAGGRIDS
                     PPTYWKGRVFFGGKDGFVYCLRAADGALVWKFQAAPSQLTHCAWEMIESVWPVHGSVL
                     VENDLVSFVAGRSCFLDEGLWFYRLDARTGAIRVKEHYDDRDPETGGPLNDRHKTLQM
                     PVASNDILSSDGKWTFLRTQKIGADGKRVEIGPVSGDFAKQGGAHKGEGQHLFAPMGF
                     LDDSNFHRSYWVYGKSFAGGHGGYFQAGKYAPAGRVLVHDDKNVYSYGREAKYYKWTT
                     TMEYTLFSTPKDAAEEAYTKEDALTPRTGNSVVLGPDGKPLDPQPPKLAVEGKAAKGK
                     GKGKAKAAPAKGPAPLAGSVRFPSNATTDPTGKGLTIEMWVLPDQANGMLVNFGGPLV
                     GFALELQQKKPSFHVRSEAKLSSIAAKEALGDGWTHLTATLSEDKTMRLYVNALQVAE
                     GQASGLLDKMPARPFLLGNSDGGASDSKVGGYSGLLDQFAYFTRALSPAEIEARFNAP
                     EVTPKDATIVCNFDNGDSRDSSGNNLHGIGSGMETGKGKVGGALWFKNSPAGGGGGQQ
                     PGSFVKHTWDRFVPIVARSMALAGNSILVGGPPDTIDEEYAFERLAARDPAILKELEE
                     QNEALEGRRGAKMWAVNVETGEQSTGLELTAPPVWDGITSANGQVFVSTVDGRLQCFG
                     K"
     gene            complement(39273..40637)
                     /locus_tag="PLP58_01700"
     CDS             complement(39273..40637)
                     /locus_tag="PLP58_01700"
                     /inference="COORDINATES: protein motif:HMM:NF039847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HRJ07267.1"
                     /translation="MHNAARANRRRFLRQTAASLGALGFPVIIPASALGKDGRPAPSN
                     RTTLGLIGCGGRGTDVVKNFLNDPRVQVVAVCDVERESDRYNKGIIKKAGALGREPMR
                     RLVDKTYDGKGCSTHEDFRELLQRADVDAVQIATPDHWHALQAVAAARAGKHIYCEKP
                     LSLTIAEGRFMSDEVSKAGIVWQTGSQQRSDVHFRMACELVRNGRVGKLKQIRVGLAS
                     DNRDNNGHAAQTSPTPAPEGLNYDLWLGPAPDAPFCPARLHSNWRWFYDYSGGNITDF
                     GAHHLDIVQWALGQDESGPVEFSNLQATWPDPGSLYTTPPAFSFEYRYADGTLVRVAN
                     QMDFGSGIAFEGELGSITCVRGGLKIEPVELRKPLKEGDLRLYESKDHFRNFVDAIQD
                     GVKTAAPIEYAHRSITIAHLANIALRLGRKSLRWDPAAERVIGDEEADAMRSRPMRGP
                     WRLA"
     gene            complement(41951..42235)
                     /locus_tag="PLP58_01705"
     CDS             complement(41951..42235)
                     /locus_tag="PLP58_01705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ07268.1"
                     /translation="MKHGIKFAVRAFVLMLATVLVLISTWAAYTFIADGLSAQMMGFE
                     REPAQGWAYWRWFYLKRSMWGILGTAGILLVFGALVAWGWVAFVRDVRRK"
     gene            complement(42232..43179)
                     /locus_tag="PLP58_01710"
     CDS             complement(42232..43179)
                     /locus_tag="PLP58_01710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ07269.1"
                     /translation="MNNANSSFVTLVAAALLLASCGKQGDRHATDADAKAAPAKASPN
                     TPSQAPSATQTVPGQSSTPDASRTAPQARQRVMAEIVSMIGKPAGGLTRDHWRQIREH
                     YWLPSFDRVIRAAGSKASTHELLQPPKDKRPLVVQLLSGAFETQDAAEQDSLLALHNL
                     ALVTAAAGGASLPAALADRQSDPNITQGDIILFEVFNDAFEDVPRTEQLGQSTLQEWQ
                     QMAASHNGVVRLLALRTFRRVAPQPEQWLEFYRSYKNERDEGILTELTDMVFETAKPE
                     AATILGEIRSRPGQALPADLAAKLDRSIDFLTKLALRAQ"
     gene            complement(43176..47174)
                     /locus_tag="PLP58_01715"
     CDS             complement(43176..47174)
                     /locus_tag="PLP58_01715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ07270.1"
                     /translation="MKADTKRAVALLLLVCHWGGMLHSQAPEWWVTRQARDPALAPDD
                     FALVNQGQLKQLARAAFLEMEARTPGGAGPQITALVQSWLAPATAPRDDYAVANIGQL
                     KAVAAPFHARLQELGVAVPFTWTAVTTDDDDFAPANIGQAKALFAFAIPDADALLPPE
                     VLAYKNTYGGLFGEDSPFVIHYADWRCNQVRTHVGETRTLGWTDTWQWHLPQGDQACP
                     QKQKTATHYAAIPYDMPADDWINSHTDPVPPFTQRTVDLEFEHTYTPDPALVTRIQEF
                     EILRQFAFPGGQPLDFNRQLKQVWWVHSGATGESLWESLYPGEDYSAKLASHGSHRYE
                     TPAQTWRVSSHDPSIPLLPAAGPGQLTPPHLFEQNAAGGPASPPQTGWTAPPAEEVHD
                     PLAALAQNGNPIPFQPLKMPASGGIVAIAQSQLEVHPMHHLPASGQPVLEPRCQGAYH
                     RGKVLISWRPGLRPQDMTAQVMNNWIQRYKVIIEETVYHADGTQPEPVTSTTDLSTLL
                     TPSSSTAQIDQLDLGAPDLEDFSPGQQRLLTLRLENLVPVVQDPDKPGGTGNPGGPNG
                     TGGPNGGPDGTGSPENPEIFTVYLETAEQNGTWKFGNKMVPELDENGHQIGSYVPSTF
                     SSLQCKLIKPAEDPEQPELSSTWPEYYWRYDGASEPDWLASALHSEHLEPADHEWEEL
                     ESLPCSPERAADRQYETAVNGRPKPALVANFSPTPLQNNPNHDPGSGSESWKDMRWID
                     YHAAKVRLCSTEVLPAGYEQSFILVKEVQKPPPYAPGQYDHTVLGTHLLKIEPGDNGT
                     QSDFTHLVDFSSEEIAKAYTQNQSFHLRLLPVDLATDLNNDGQITSADSSLREAATKS
                     GASDADKEKGTEYLFINDKMSNGVWDADDDGVMSYSYGSGYSTMPKPPAAHKDDDDAQ
                     ALKVSVGGLNTGVVWFDHSVIDKLEFFKTKECTASDKLNITASSPFDLSTGSLPETIY
                     VRLRDDWAGVDSEGTLRMFVGKTINETWAELKLPLTVVRDFGAKHFFHAARDYIMENN
                     ARVCIRDHGYPFGPNPSVIFRLCVMREEATKMTAFDALAGGQAGIEMSYFALPAVPRA
                     PAVMVNGNQCFWSAGWDEKNPFDLPNMLGNIADKCHGRVIRGGVTATTSSDNFDNTTK
                     PAAGSPLAGPDPIPAGTLAGPDGVPGTSDDIANPYAGDPGGKYLGCNSGAWTFAAGRA
                     GGSDALGGLSTNYASAERADKAHQMIGYAKGEETGKGCIFTATQIKGVGYGPIVKSDA
                     STAGVPALTPSADAGAIKLFILDSGAGSLALMHLDPTGTMRGAYMGRKTNFGFPYYVN
                     NYLALDPAPPRP"
     gene            complement(47245..48285)
                     /locus_tag="PLP58_01720"
     CDS             complement(47245..48285)
                     /locus_tag="PLP58_01720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ07271.1"
                     /translation="MKPHALSPRLSIAAFLGLIPVVLQAQSELSAEHLTIQQSARFGT
                     LIDDTGGAGFTLDVLQTQYETEIMVTVTPASYTTTEYQEIWDWVDMGSQQEVWGMHSV
                     EYYQPPTYDEDGNVLDPGGPYWLDEPYLISNDWVSDWQYAVVGHDYFQVTITPEPYEE
                     VQYITNYTHPVVRFTGSRSNTEWLWRNPDAPGFHRPLMKLGAAGLSFPLPFESADHDR
                     VSLLNYYQMLYSKSNLAADDAQRQSRGSRVTEEGVTVWHDESGAPGAESRLQNPAWSA
                     EVRIQPNTLEIQRKQPASEGEASVITALSADFATFGGGASFAREVSFHGAVRIQPRGD
                     LSMGAFTEGPQP"
BASE COUNT         8658 a        15377 c        15450 g         8891 t
ORIGIN      
        1 gcagcctacc ccccgcaggt cgcgcaggtt ccgccgcccg ccgcgcctcc ccaggaggcc
       61 gcccgctccg cccctgcgga cactgcgccg gcaggcccca ccatcaactc gcccatggtc
      121 ggcactttct accgctcggc cgcgcctgga gaaagctcct tcatcaacat cggcgacccg
      181 gtggatgaga acaccaccgt ctgcatcatc gaggccatga aggtgatgaa cgagatcaag
      241 gccgaaatgc gcggcacggt ggcccgtgtc cttgtcgagg atggcaagcc cgtgcagtac
      301 gggcagccct tgttcgaatt gaaagcctga cagctccgag ccagccgacc gccaaccggg
      361 tcaagccgcg caaaaagcac agaaatccgc ccattctccg catgttccgc aaagtcctcg
      421 tcgccaaccg tggtgaaatc gccgtccgca tcatccgcgc gtgcaaagaa ctcgatgtca
      481 aaaccgttgc cgtctattcc gaggcggatg tggactcgat gcacgtccac ctggcggatg
      541 aggccatctg catcggcccc ggtccaagca ccgagagcta tctgaagatc agccgcatca
      601 tcagcgcggc ggagatcgcc aatgtggacg ccatccatcc cggatacggc tttctctccg
      661 agaaggagga gttcgccgaa gtgtgcgaca agtgcaaaat caagttcatc ggccccagcg
      721 ccagggtcat ctccatgatg ggcgacaaaa acaccgcgcg cgccaccgcg cgcgcggccg
      781 gtgtgccggt gacacccggt tcggacggca tcatccacaa tgaggagcag gcgcgcgaat
      841 gggcgggcaa gatcggctac cccgtcatca tcaaagcgac ggccggcggc ggcgggcgcg
      901 gcatgaggcc ggtgctcaac gaggccaccc tggtctccag cttccaggcc gccagccttg
      961 aggctctcaa atgcttcggg gacggctcca tgtacatgga aaagctggtc gaaaagccgc
     1021 atcacattga gttccaggtc gtggccgaca gccacgggaa cgtggtgcat ctcggcgagc
     1081 gcgactgctc catgcagcgc cgcaaccaga agatcatcga ggagtgcccg agtcccaaga
     1141 tcagcgacga gctgcgcaaa aagatgggcg atgccacggt gaagctgtgc aaggagatcg
     1201 gctatgagaa ctgcggcacc atcgagttcc tcgtggacaa caaccgcaag gatttctatt
     1261 tcatggaaat gaacacccgc atccaggtgg agcaccccat taccgaggag gtctatggat
     1321 gcgacctcat caaggagcag atccgcatcg ccgccgggct gccgctcagc gagcatgtgc
     1381 tcaccgccgg cgcgcgcggg cactccatcg agtgccgcat caacgccgag gatcccgcgc
     1441 ggaatttcgc gcccagcccg ggcaggatca atctttggta cacctcaggc ggacgcggcg
     1501 tgcgcgtgga cacgcacgtt tattcgggct acgaggtgcc gccttactat gattcgatga
     1561 tcgccaagct catcgtcaca ggcgccacgc gtgacattgc catccgccgc atgaggcgcg
     1621 cgctgggcga gttcaccgtg gagggcatca agaccaccat cccgctccag tcgaagatcc
     1681 tcaccacatc cgacttccag aacggcaact atgacatcac ctgggtggaa aatttcctgc
     1741 gccaggaagg catgaagggc tgaacccgcc ttccgggcgc tgcatcaggc atgagagaaa
     1801 aaggcggcgg gaaaccccgc cgcctttgtc gttctcagtt ctcgcgcagt tcaaaccagc
     1861 cgccgagaac gggcaccgtc ttcttgggca cctcgccatc ctggggtcgt ttttccagca
     1921 ggaagtgccc gacgccgtag gtcttcattt ccacatcgtc acggatgagg atgccgtgga
     1981 agacggcgtc gcggatgaga tttcctgtgg cgccggtgcg ctggtctggg tcggtgaggc
     2041 ggaaagtccc gtcgaacaag cctgtcttgg ccgccagctt cttgatgagg gtgctggtcg
     2101 ggccggccgg tttgggattg gcgattttgt tgccatccag aatgccgagg atttgatcgg
     2161 gatcctgggt gctggtcagc aggtcgccct gctcgaaatc gagattcaga ttggtctggg
     2221 tgccgggcga ggccaggccc atgatcagca ccttggtctt ggtcggatcc acataaatgc
     2281 cgcccgtggc ctccagcggc acaggcgagg cctccggcgt gccaggcagg ccaaagccgg
     2341 cctggtaggc gcgggtcttg gtgttggtgt tcgcgggtcg cacccagttc acatcaccgc
     2401 tgaggctgtt gtcctcggca ttgttggtgt ccacgggatc aatgacaaga gacccccaga
     2461 gcgaaccggc cagcggtttg taaagcgtct ggaagaccac catctgcccg gcgaatccga
     2521 ccgaggcggc gcaggtgatc gtctcgccgt cggcagtgcg cccggcgatt ttccatttgc
     2581 cgtttttgtc cactgtgaac gcgccgtagc tccagccttg gggaatgtgc tcctggccgg
     2641 gatgtcccgg atcccccagt tccaggccga aggtatagta cccctgaaag gcggcggctt
     2701 ccgcgggcac agcgtggcgc cagccattga cggggatgcc gttggcgttg cctgtgagca
     2761 gccgttgagt ggtgacgatg ttgtccatga tcatgtcgaa cgctagggtg acattggtgc
     2821 cgttcttgcg cttcaaggtg acggtgtaat tgggagggag gacttcagcc gcgtccacct
     2881 cgatgccgcc cttgagcgga atgcgctcgg ctcctagggt gagggtgccg gtgagcgcgc
     2941 ctgtggcgct gatggtcacg tcaatgcgtc cgcccagccc gtcattgagc gccgtgtcgg
     3001 gatccacggt gcctgaatag gtgccggcga gattggcggg gaagtctttg atgatgatcg
     3061 tttcaacgac ggggtccgcc ttctgggtgt tggccgcgcc taatgtcacg tcccggccag
     3121 ccgggaaaga cttcgtgggt ctgccggtga ttttgccggt cttcggattg actttcagcc
     3181 ctgcgggcag gttggcggcg gtgtaggtgg tgggtgcttt ttctggcgcc gcgtccacca
     3241 tgatgtcaaa gccttccagc ggaaatccat aactgccacc gatgatccca tccggcatgt
     3301 tctggggatc gtcgagcacc ggtttttccg tgaatacctt cagttcagta tggccgccgg
     3361 cgcgcatccc gccgggaccg gtgacctggc aggagtagag tccgctgtcc ggctcgtcga
     3421 ggttgccgat gaccagggtc ggagtctgcg ctcccttgac gcgcccgtct gcggtgttgg
     3481 ccacgggaac cgcgccaagg aaccactggt aggtcaggcc gttgccagcc gccgaaacct
     3541 tcatggtggc ggatttggtt ttgccaacga cgaggaccgc cgggggcgtg tttgcctgga
     3601 cgacgccgag ctgcgctgga ttgctgatgc cgaggatttt gccgactttc accgccacgg
     3661 tgtaggcacc cgcctctttg agcgtgacgg cggggatgtt gaagccggtt ccgagggctc
     3721 ccttgatcgc tccgttgttg cgccgccact ggtaatccgc gttcgtcaat gccacgcttg
     3781 agctggccgc catcgcgtca aaagcggctg cctcgccgac gttcctcatc agggaatccg
     3841 gatggtctgt gatggtgggg tctttcaccc cggtgccggt gacattgacg atgaagggat
     3901 tctcatcgcc gtcattgctg atgatggaga cctgcgccac acgcgggccg ccttccttcg
     3961 gcgtgaactt gacgccgaat gtcgtcgtgg cgttctgtgc caggctggag gctggtttcg
     4021 gggtgctcaa cagggagaac tctcccgcat tgatgcctcc aagaaccggt ttgatgtcgc
     4081 gcagcgtgtt gaggcccgcg ttgcggatcg tgaagatcag catggtgctc tccttggccg
     4141 caacgtccgc cacgccgaag tccaccgccg ggccgttttg cgcgagctgg ttcgcggccg
     4201 gctgctcgac gcggatgtcc gatccgatga aggcgatgcg cggctcgtcc ccgtcctccg
     4261 tgtcggccgc cgtgtagagg agctggccgc ggaagggggt gaaaccgccg gggcttgacg
     4321 ctgtcgggat gccgctgatg tcgttgatca tccgggtgcc ggccaccgtg ccgtcggaga
     4381 tccacaactc gcgtccgttc acgccgtcgt ccgccgaaaa gcacagcacg ccgtccacat
     4441 ccttgagacc cgtgatgccc atgctggagg cgcggttgcc gccgttgcga aaatctgtga
     4501 gcataaccgt gcctgcgagg gttccgttgc tcgtccacaa ctcgcgtccg tcaaggccgt
     4561 tgtcggcggt gaaaaagacg agcttgccgg aggctgtcag ctcctgcggg tcggaacccg
     4621 gggctccggc gttgatgtcc cgcaccaaga cggtgcccgc ctcggtgccg ttgctcctcc
     4681 atagctcggt gccattgacg ccatcggtgg cagtgaagaa gaccactttg tccacagtgg
     4741 tgatttgccg ggggagcgag tcacctgtgg gattgatgtc cttcacgcgc acggttcccg
     4801 cgtccgtgcc gttgctggtc cacaattcac gcccgccttg ggattcgttg cgtgccgaaa
     4861 aataaagcct ctcagaagct cccgaaccca tcaccgccat ggattcattc tcctcgttga
     4921 tgctcgaaag cccgccggga gcaatgtctt ttaccagggt taccgtcgtg cctgtgccgt
     4981 tgattttcat cagctcgcgt cccacgccat ccctgccagc gaagaaatag acttcgtttt
     5041 tgaaaggtgt gaactgcctg gggaatgatc cctccggacc ggtgacgagg tcagccacct
     5101 ggaccgtgcc cgcttcggtg ccattgctcc tccacaactc gcgtccggtg gtgccaaatc
     5161 cggagccatc ctccgcagta aagagcaaga gtccgttcag atcgaaaaga ttctcagggt
     5221 ttgacggccc ggtgggattg atttccttga cctggaaaac cgccgggttg gcggtgggat
     5281 cataaatcca gagctcgcgg ttcgcattcg cgttctcggc gctgaagaac acgttcggcc
     5341 cgcacggggt gatccaaagg ggatcggaac tgttgggcgt gggcgggaaa ctgccatcgt
     5401 ctccagggaa aatgtcagcg aggcgcatcg tgccggcatc cgagccgtcg cttttccata
     5461 attccctgcc ggtcacgccg tcatccgctg aaaaatagac atccgtgccg gccacggcga
     5521 actccccgga tgaggatccc cggctgccgg agcggaagtt aaagaccatt ttcgtgccgt
     5581 ttgaattgcc cgctggagag ttggtcaggt ccgtctccca tagctggctg ttgttgagaa
     5641 ggaagaacac cttcgagccg agccgggtca tgtggctggc cgtgctttca atgcccaggc
     5701 ttttgatgcg agtgaccgcg ttgggcagcg acgcgccgga ggcgcaggtg tagaggccga
     5761 ggttgttgga ggagtcaacc gcggtgaaca gcagggtggt gttgttgaaa ttgacgaaat
     5821 tccccggatt cgaaccgccc gtgccggcca caatgtccgc gacccgggcg gagttttcat
     5881 tgcctgtgcg gccattggtg gcgtgcagtt ccactcccga accatcatcc gccgtgaaga
     5941 aaagattgct gcccaccacc atgagctggc tgggattcga gctgtcgccg ccgacaaaaa
     6001 tgtccttgag ctgttccggg gtgttaagag ggtttccgtc actgcgccac agttcgacac
     6061 cagagatcgc tccgtcggtg gcgctgtaat aaaggtaggt gccggcttgc agacctgtga
     6121 tcgtcatcac cgtcatgtgc gagggttcgg aactgctcgg tccggggaaa aggtcaaaga
     6181 cctcctgggt tccgcccgct gtgccgtcgg tgcgccagag ttccaccccg ttgcccgaaa
     6241 aggccgagaa gtaagtcagc ccgttcatgt tcacatattg cggcgcgatt cggccgaatt
     6301 gctggaacaa gggttctgac acgccaagtc tttccacacc cgtgcccgca gggttgagcc
     6361 tgtaaagacc gacatcattc tggccgtctg tcgcagtgaa atacaaaatc gccgaagggc
     6421 ctgtcccgga gacataaaaa tggtctatgg ccgagtcgcc tcctccgggg cgtatgtcac
     6481 tcacgagcac ggtgccggcg tctgtgccgt ttgacctcca tagctccaga ccggcggtgc
     6541 cgctgaatcc ttggaaatag agcgtgccgt tgaaattgaa caatttctcc ggtccgtctg
     6601 agccaaagaa acccagatcc ttgaccagat ttgtccccgc agccatgccg ttgctcgccc
     6661 agaggttgcg cttgggcgga gaagcgtcat tggcggcgga aaaaaacatc tgagtgccgg
     6721 caggagtcag attgaccggt gcggagccgc cattgcccgg cacgatgtcc ttgatgatgg
     6781 ctgttcctcc cggtgtcccg tcacttttcc agacctccga gccgtgcagg ctgtcctgaa
     6841 cggaaatgat cattatctct ccgctgccag gattcacttt ggccagggag gcggcctcgg
     6901 tgggtgcgga aaggcccgtc gggttgatgt ccttcaagag cacggtgcct tgtccccagg
     6961 cgctggaact gataagaaga attccagccg aaattttttg gatttttttc atgattgtaa
     7021 tcacagagta ttgacagcga cgaacgaagg caatcctatt ttcaaaagcc gggtttgacc
     7081 gcccatgact ctgcctcctt gcaagtttgg accattcccc cgccggaaat gctcaaccgc
     7141 cgtctccgcg cttgcccctg cggcgttccg cgtccatgaa agcggccctt tatgagccgc
     7201 actcacaaac tcgcagttct ccccggagat ggcattggcc cagaggtcat ggcccaggcc
     7261 ctcctcgtcc tcgacagcat cgccgccaaa tccggcattc attttgaata caacccccag
     7321 cttgtcggcg gtgccgccat tgatgctgcc ggcaaagccc tcccggctga gaccatcgcc
     7381 gcctgcgagg ccagcgatgc catccttttt ggctccgtcg gcggcccgaa atgggagcac
     7441 ctgccgccca acgagcagcc ggaacgcggc gccctgctgc cactgcgcaa gcattttggc
     7501 ctcttcgcca acctccgacc cggcatttgc taccccgctc tcacagcttc cagccccgtg
     7561 cggcctgacc tcgttgaggg tggctttgac gtgctctgcg tgcgcgagct cactggcggc
     7621 ctctatttcg gccagccgaa aagccgcgac accctgcccg atggcgacat ccaggtcatt
     7681 gacaccatgg tttacaaaaa gagcgagatc gtccgcatcg cccacgtcgc cttcaaagcc
     7741 gcgcaactcc gcggcaagca cgtcaccagc gtggacaagg ccaacgtgct caccaactcc
     7801 gtcctgtggc gtgagacgat gatcgaagtc gccaaagaat accctgatgt caccctggcc
     7861 catctttatg tggacaatgc cgccatgcag ctcatcaagg ccccgcgcag cttcgatgtc
     7921 ctcgtgaccg aaaacttgtt tggtgacatc ctcagcgatg agatggccat gatcgccggc
     7981 agcctcggca tgctcgccag cgccagcctg ggccagccga aaggcgacgg gctttacttc
     8041 ggcctcttcg agcccagcgg cggcaccgcg cccgacatcg ccggcaaagg catcgccaac
     8101 cccatcgcgc aaatcctctc cgccgccctc ctgctgcgtt tctcgctcgg cctggaaaac
     8161 gaggcgctcg ccatcgaaaa cgccgttaaa aatgtcatcg gcgacggtct gcgcaccggc
     8221 gacattttca ccagcgcacc cggcacccgc ctcgtcaaca ctgcggaaat gggcgccgcc
     8281 atcgtcgccg cgctttgacc tttccgccgt agtgcccttt aaaaaatcaa atttcgaaac
     8341 gccgcctctg gcgatagtct tccgcgatga ctcggacact ggttctcctc agcggcggca
     8401 tggactccac cgccgctctc cactgggcgc gccgggagca tgaggtggcg ggtgcggtga
     8461 gctttgatta cggatcgaag cataatgcgc gcgaaatcga actcgcgcgc tggcagtgcg
     8521 agaagctcgg cgtgccgcat gacgtgcttg cgctgccttt catcaacgag cacttcagct
     8581 ccgacctgct gctcagcggc ggcaacatcc cggagggcca ctacgccgat gagaacatgc
     8641 gccgcactgt ggtgcctttc cgcaacggca tcatgctcgc catcgcctgc ggcctggcgg
     8701 agagccgggg cgcgcgggcg ctggtcatcg ccgcgcacgc gggggaccac gccatctatc
     8761 ccgactgcag ggaagaattt atgcaggcca tcgccgccgc catgcgggcg ggcacttatg
     8821 cgggcatcga gctgctgcgc cctttcattc acctggacaa ggcggccatc gcgcggctcg
     8881 ggcatgacct gggcgtggac ttcggcatga cctggagctg ctacaaaggc gggggaattc
     8941 attgcggcgt ctgcggcacc tgcgtcgagc gtcgagaggc cttcctcaag gcaggcctgc
     9001 ccgatcccac gcggtacctg gatgagggcg ccctgcccgc gccggcggtt ttgccgcctt
     9061 caagctgaag ctgtttcacc cagggtgggc ctgccttgtc agagatggaa gatcaaacac
     9121 ggcagccgca tgaactggaa ccgctctttc catcttgcca gccaggccat cccctcgtag
     9181 tcagcctgga aagaagcccg tcccgctgtt cgtctcccat tcccccattt tcgccccatg
     9241 tcagacaccg ccgccgccgc cgccctcgta gatggattcc aaaagctcaa agccgccttg
     9301 agcaggcgca ttgtcgggca ggaggccgtc attgagcagg ttttcattgc catcgccgcc
     9361 ggcgggcaca gtcttctgga gggtgtgccc ggcctggcca agacgctgct ggtcaaatcc
     9421 ctggcggacg ccatgcatct gagcttccgc cgcatccagt tcacgcccga cctcatgccg
     9481 gcggacatca ccggcaccga gatcatccag gaggatgccg agaccgggcg acgcaagctg
     9541 gttttccagc ccggccccat tttttcccag atcattctgg cggatgaaat caaccgcacc
     9601 ccgcccaaga cccaggccgc gctgctggag gccatgcagg aaaagcacgt caccgtcggc
     9661 caggagacct tcgatctgcc gaaacccttc ttcgtgctcg ccacgcagaa ccccatcgaa
     9721 caggagggca cctatccgct gcccgaggcg cagaaggacc gcttcctctt cctcatcaaa
     9781 gtggactacc ccagccgcga ggaggagcgc gaggtcatcg cccgcaccac cggcggcgcg
     9841 caggaggaga tcaaggctgt cgtcacagcc gaggagctcc aagccgccca ggcgctggcc
     9901 cgcaaggtcc ccgtgcccga ccatgtcact gactttgtgc tcgacctcgt gcgcgccacc
     9961 cgcccgaacg agcctgacgc catgccttat gtgaaggaga tgctcagttg gggagccggg
    10021 ccgcgcgcca gccagatgct cgtgctggcc ggcaaagtcc gcgccctctt gcaaggccgc
    10081 acccacgtca cgcttgatga cattgaggcc ctcgccctcc cggccctccg ccaccgcctt
    10141 gtgcccacct tccacgccga ggccgagggc gtgaccgtgg acatgatcgt cgcggagctg
    10201 atcaagcgcg tgaaaaagcc ggaggcgcgg gttttgtgag gacggggttc ctggttcttc
    10261 gtccttggtt attggtttcc aattccgccc gccgtggcct ctctctctga catccttcac
    10321 gccgaagaca tcacgagcct gcaaaatctg cagctctttg cccgcaccgt ggtcgagggc
    10381 ttcaccacag ggcaccacgc ctccccgcac aagggcttca gcgtcgagtt ccgccagcac
    10441 aggccttatg tgcgggggga tgacatccgc cggctggact ggaagatctt cggccgcgcc
    10501 gaccgcttct acatccgcga atacgacgag gagaccaacc tgcgcgcgac cctcgtgctc
    10561 gacgccagcg gcagcatggg ctaccgcggg cagaaaggcg tgttgaagtt cgactacgcg
    10621 cgcaagctgg ccgccgccat ggcctacctg ctcatgagcc agcaggacgc cgtgggcctc
    10681 gtcactttcg acagcagggt gcgcgagttc atcccgaacc gcacccgcat cacccacctg
    10741 caccacctgc tggagacgct ggtgaaaacc gagcccggtg aggagaccgg cctcgctcct
    10801 gtgctggaat ccctggccca gcgactgaag cgccgcggcc tcgtcatcct catcagcgat
    10861 ttttttgacg acgcggacgc catcttgaaa gccatcggcg tgctgcgcaa aaagggccat
    10921 gagatcattg ccatgcagct atgggaccgg gatgagatcg agttcccctt cggccagtgg
    10981 tcgcgttttg agaatttgga gcaggacgag gacttcctca tgctcgaccc cgccgccgtg
    11041 aggctgcgct acctcgaagt gcggcgcgcc tttgaggagc agctcaagga aggtttccgc
    11101 aagcaccagg tggaccacct ctccctgccc accgacgaat cccacgccgc cgcgctgcgc
    11161 gcatatctcg cgctgcggat gcggtgacgc tccggggtca cgcgcgcggt tgcctcgcgg
    11221 cgtatttgct gctcctagtc gcgtcatgtc ccaaccaccc cgcccctgga tcattgccga
    11281 gacgaactgg aaacaggtga aagccgggcg ttacgaagtg gccgtcctgc cctggggcgc
    11341 caccgaggcg cacaactggc atctgcccta tgccaccgac agcctgcaaa acgaggccat
    11401 ctgcgccggg gccggacgca tcgcctggga ggctggcgcg cgcgttgcca tcctgccgaa
    11461 catccccttc ggcgtgcaga ccgggcagct cgacattccc ttttgcatca acatgaaccc
    11521 ttccacccag gccgccgttc tggaggatgt gatcacctcg cttgaaggcg tcggcgtgcc
    11581 gaagctcgtc gtcttcaacg gccacggcgg caatgacttc cgccagatcc tgcgcgagct
    11641 gcaggcgcgc catccgcgcg tttttctctc cctggtgaac tggtttcaaa ttcccggcgc
    11701 gcaggacatc ttcaccatcc tcggcgacca cgccgacgag cgcgagacca gcctcatgct
    11761 ccatctgcac cccactctcg tgctgtccaa agaaaactgg ggaccaggca cggaaaatcc
    11821 ctggaagtta aaagccatgc gcgagcgctg ggcctgggcg cagcgcgcct ggacgcaggc
    11881 cacaaatgac accggcgtcg gcgatccaca gcactccacg gcggaaaaag gccgtttata
    11941 ctttgagctg ctcgccggga aattcgccgc gcatctcgtc gaactcgccg ccgcggacac
    12001 cggcgcgctt tatgacccaa cgccggtctg agagcttccg cgtggctgga aaataacccg
    12061 cgtcctcaag acagggacat cccttgctca tcacgacagt ctccggtggt gggaaggatg
    12121 acatggctgc cgtgacgtga aaagtggcgc cgcatcacac cagccatgct tgatcgaatc
    12181 ctttcctgcc tcttcctcct tccaatcctg gccggcgccg ccgagctcgc ggcatccgcg
    12241 ccagccgccg aagatgaggg acgggaaccc gcgtcagcga acacccagtt cccccccgtc
    12301 gtcgtcaccg ccacgcgcga tggcatcgaa gcgctcaagg ccccgaacca ggtccgcacg
    12361 ctcgactccc gcgagatgct ggagcgccag gcgcgcacag tcaccgaagc tttgcgggaa
    12421 acacccggcg tgaatgtgca aaagacctcc aacgcccagg gttcgccctt catccgtggc
    12481 ttcacaggct tccggaatct ggcgctcatt gacggcatcc gcttcaacaa ctccaccttc
    12541 cgcgaggggc ctaaccagta ttggaacacc attgactcct atgccataga ccatattgag
    12601 cttgtacccg gccagggttc cgtgctttac ggcagcgact ccatcggcgg cacgatcaat
    12661 cttttcacca aagggccggg ctttcgcgat gaggagtccg gtttcttttt ccacggcctc
    12721 agttcctacc gcgcctccac cgccgagcgc agccacatgg gccggcagga gcttcaattc
    12781 ggccagggcg gtcgctgggg cctccacctt ggtgccagct tgaaatcctt cggtgatgtg
    12841 cgcggggcgg gcgtcggcat ccagccgcac accggctatg acgagtgggc ctatgacatg
    12901 cggctcgacc tcgccctcga cgaaaactgg accctcacag gagtccacca gcagctccgc
    12961 caaaacgacg cctggcgcac ccatcagacc acatcgggca tccagtggcg cggcaccacc
    13021 accggcaccg atttgaagcg cgcctatgac caggagcgca cgctttccta tctccgtctg
    13081 gcgggtaaaa atctcgactc cttcgtggag tccgccgcct tcaccgtctc cctgcaaacc
    13141 gccgacgaat acgagcaccg catccgccgg ccggccgaca accgcattga cttcacacaa
    13201 gtggaggtca ccaccctcgg cctcgacctc cagctcgtca gcgtcacacc cgtcggacgc
    13261 ctcacctacg gcgtggatta ttaccgggac tgggtcagca gcggctccca gcgctaccgt
    13321 ctcaatggca cctttcattc ccgcggcatc caggggccgg tgggggacga tgccacctac
    13381 gacctgctcg gtctctatct ccaggatcag ttcgacctcg gctcgcgctt tcatgtcttt
    13441 ctaggcggcc gcctcaccca tgccgcagcc gaagtgggcc gcttcaacga ccccgccacg
    13501 gccagggtgg tggactctta cagtgatgac tggcagaact tctccggcag tctgcggctc
    13561 gtcctcgatc ttgatgagga ggaccgcttc aagctctttg gcggcgtatc gcagggcttt
    13621 cgcgcgccga atctctccga cctctcccgg ctcgacatcg cccgcagcgg cgagcgcgag
    13681 atcccatcca cgggccttga tcccgagaag ttcgtcaatt tcgaaatcgg cctgaaagcg
    13741 gagacggagt ccatcagcgc cggcctcagc tactttcaca ctctcattga cgacatgatc
    13801 gtccgccgcc cggtcggtgg tggcgacgtg ctcaaagaca acagcggcac cggctttgtc
    13861 cagggcatcg aggtcaacgg cgaatggcgg ctcgatccgc aatggacact cttcggccac
    13921 ctgacctgga ccgagggccg cgtggatcaa tacccgcgca ccaacagcac ccaccagcgc
    13981 gccgagccgc tcagccgtgt cgtccccctg atggggaggg caggcgtccg ctggcagagc
    14041 agcggccgcc gcctttggac cgagcttgtc tgcatcgcgc acagccgctt cgagcgcatg
    14101 aacacaggcg accggcgcga cacccagcgc atcccgccgg agggcaatcc aggatttgaa
    14161 aacctcatcc tccgtggtgg ctggctcgtc acgaacaatc tggaactcaa cgtggctttg
    14221 gaaaacctgc tcgaccagga atatcgtcac accggctccg gcagcaacga acccggcttc
    14281 ggcgccgttc tcggcgccac cttccgcttc taagcatcgt tcagcagccc ttcgatgagc
    14341 tggacatgcc aggtccgccg gcactgccgt ttgcccctgc catatccccg gccatctttg
    14401 cggccccatg tcccgccgcc ccgcctcccg cctgcccagg ccgttcgtca tcctcctcgt
    14461 cctggccttc gccgcctggc actactggca ggaggcacag cccccgcctg acgcgccgcc
    14521 gcgcaccggc cccgccctca gcagccacga ggctttgcat ggcgcgcgcc tgctcgatca
    14581 ccgcgacaat gacggcgaca gttttcaaat cctccacaac ggacaggccc accacttccg
    14641 cctctacttt gccgactccc cggaaaaacg ccgccaccag cacaatggcg accggctgcg
    14701 cgaccagggc cgctacttcg gcgggctcga ggaggcccgc accatcgcgc tcggccttga
    14761 ggccaaaaag ctcgccgaga atctgctgcg cagccgcccc ttcaccatcc acacccgctg
    14821 gcatcaagtc tatgacagcg gccggtatta cgccttcgtc atctttgacg acggtgagga
    14881 tctgggtgag aagctcgtcc gcgccggtct cgcccgcatc cacaccaccg gcgctccgca
    14941 ccccgacgga cgctccgcgc aagcctttga aaagcacctg cgccgcctcg agcaggaagc
    15001 caggtccgaa gcccgcggcg gctgggcgcg gtgaacccgc cccgatttgg cgcgtgacgc
    15061 tcgtccccga agaagcgccg cttcgaaatg cgcctcgctc tatcaaatga ctgcgtcttc
    15121 tctcacggcg gcgggttgag ttgatgcgcg ggcgcgccgc ggtgctggaa gataaaagct
    15181 tcaggtgaaa cgcggttcag cggcgtgtcg gcgtatcccg ccccggggtt gatgtaaggg
    15241 ccttcccgga tttcaaagtg caagtgggcc aaatattgcc cctccgcaga gccgactgtg
    15301 gcgatgcgct gcccgcgccg cacccgcgcg ccgggttcgg cgaggatgtc ctgcaaatgc
    15361 gcataaacgc tctggatgac tcgcggcggt tccacagggt catccgcggg gacgcgatgg
    15421 gcgaggatga gcatttttcc ccagccgggg ccgggggacc cggcatagac gacgcatccg
    15481 tcggcgatgg cgtggacggg atcgccgagg tcggagttgc caccgccgat gccgttgagg
    15541 tcgtcgccca ggtggcgcgc gacgcggaag gggcgggcgt tgtaggtcag cgcgccgtgc
    15601 tcggagccga ggggatggtc aaaccgcgaa gccagcggca gggcggcggt ttccgcggct
    15661 ccgaggcggt gcagggcggg gtcgaaagac gcggcggacg ggcgtgacgc gacgcggagc
    15721 gcgcgcgtct catgccaaag aagaccggcg ctggcggaga gcgcgaggaa tgtcagcatc
    15781 tggcggagac ggcgcatggc tggggctgga ggcgggtcat tcccgggcgg ctatgccgaa
    15841 ggcgcaaacc tcgtttttgt tcgcgcacag gcgcgtgagg cggaagtcgg cgcagagggg
    15901 cggcgcgttc tgcttgagtg tgtcgagctg gccgtactcg tccgtgcctt tgaatttaat
    15961 ggtgacgatg aacgcgcgca tgaggcgcgc gcgcagccag tcgagcagga ggtccatgct
    16021 gcgctgcggg gcggcaatga tgtcgcacaa gagccagtcc actggctcgg gcgggcggaa
    16081 tttgaaggcg tctccctgat gaaaatgcag ccttggatgc cgcatgaggt cggcgcgcag
    16141 cggactgcgg tccacggcgg tgacatgcgc gccgcgcagc agcgccacat ggctccaact
    16201 gcccggcgag gcgccaaggt ccacgcaggt ctcgtttttc atgatgcggc gtccgaggcg
    16261 catttcggcc tcgatgactt tggcgaaagc ccggctggga gctgtcttgt cctcggcggg
    16321 agttatgtgt ccggccgggt atgcggagat gatggagcgc agctcatgcg gctccggcgc
    16381 ggcactcaag gaaagcaaac ccgagccagg cgaggttaga acggcctgca ccagtgaggt
    16441 gctcctggtg aacggttcct gactctcctc ctggcagcgc attagtgcgc ggcgctgctt
    16501 tttcagtttt tcagccagcg cggagcggat caaccggcaa cggtgctctc ccgccctgcc
    16561 ctctccatag gcgggccaga ggtgcaggcg ccaggtgcct tcttcgggca ggactccgac
    16621 gatgctttga aggatctgat ccgcccacgc attgaccgag gcggcctcca cgcgccgcgc
    16681 gcgcggcagc gtctggcgcg cgaaggcgag gtgcgcgggc agattctccg agcggagcca
    16741 cacaccctcc atgtccgcgc cgccaagctc gccggtcagc ggctcgatgg cgtcatcggc
    16801 ggcgaggaag gtgtgcatcc cgtcaacaga aaaaaccgcg caagggcgag agggcgggga
    16861 tttcatcgca cacaccggcc aggttgcgca cgccggaggg gatgtgctgc tcaaagtggg
    16921 tcttgccctg gttgcgactg agattggcat aggcgccgag ggcctgcatg aggcgctgcg
    16981 cggcgcattg atagaccacg ccgcgcaggt ccggcagcgc cagtccgcgc aggccggcgt
    17041 agtgctccag cagctcgtgg cgctcggcgc ggcccagggt cacatagggg tcgaagagca
    17101 gggaggccag atcgtaaccc gcgagtccgg ggcgcagtcc ctggtagtcc accagccagg
    17161 ccgcgccatc gcgcaggagg atgttttgac tctgaaaatc acggtggacc aggcagcggg
    17221 gcagggtggc aaggccggag cgcagcgcgg agagcgcctc atgcgcgggt tgcacctcgc
    17281 tcgtgtcggt ctcccgccgc agataaccgc gcacgaagtg ctccaggaag taattctgct
    17341 cccactcgta gagaccttcg tcaaagccgg gctccatttc ggcgatgtcg gcggaggtga
    17401 gatcatcctc gcccacggaa tgaatctttg ccgcctcggc caatgcggaa agatagagcg
    17461 ggcggcgcgc ctcccaggga tcgtcgcggt gggagtgcag gtcgtcacgc cccaggtctt
    17521 cgagccagac gaagaggcgc tgctcgtcga agccgaggat ggccggcaca tgcgctccga
    17581 gggcggcgag gcggcgcgtg gccggcacga agcgggcatt gtcccgccgc gcctgggtgt
    17641 agatcatcag caccatcggc ggctgcgcgc cgcctgccca ggtgaagcgg taaaagcgcc
    17701 ggtcggagcc gcctttgagg atgggctcca cctcgcatgg gcagtccgcc agggcgggga
    17761 aacgctgacg tgtcaggatg agcagagcct cgtgttcggg tggcatggaa acgggaactt
    17821 aaagccagcg cgccgcccgc cgcaaccacc ggcctggttt tctcgtccgc ccgcgtcccg
    17881 aagccgcgcg cgaaaatgcg tttgccaccc ctcccgccag actgcattac tggtgcccgc
    17941 ccccagactc atttttcatg caatttgttt tccccaccca cctcaagctt ggcgaggagt
    18001 tcagcgaagg cttccaccag gagctgctgc gcggcctgac ccacaagctg aacaacctgc
    18061 tcgccgtcat ccagggcttc agcagcctca tcctgatgaa cgaggacctc gaccccgccg
    18121 tggccgagaa catgcagcac atccgcgagg cctccaccgg cgtcaccgcg ctcagcgagc
    18181 gcatccgctc cgccggtggc tgcgccaagc tcagcccgca gcctctcagt ctgaatgact
    18241 accttggcgt gatcgagggc gcgctcaccg agcccttccg cgccaacggc gtccagctcg
    18301 aggccgacct gcagcccggc ctgccgccca tccatgtgga ccccaccaag ttcaaggaca
    18361 tcctcgtcga ggtgctgaaa aacgccgccg aagccgcagg caaggcgggc gggcgcgcca
    18421 tcctaaaaat caccggtccc ggcggtctca cccccgccga ccagcggcgc gtggacatcc
    18481 tcgtgagcaa caccggctcg caaatcccgc cggagaagct ggcggaggtc ttccgcccgt
    18541 ttcacggcac gaaaaacagc aatcacctcg gcctgggcct cacgattgcc gccatgctct
    18601 gccatcagat gggtctgcaa atcggcatcg cctccgagaa caacacgacg acggtgtgga
    18661 tcagttgccc gacggcttga tgcttacgcc cactccttga gcctctctgg aaaggggcag
    18721 ccggcgcagc ctgaatcgtc ctccaggcag aagtcctcat agacttgcag cagaccctgc
    18781 tggtggtgga gttttttgcc gaagtctttc ttgagcgtgc tgttgccaaa caggcgcgca
    18841 accgcgcggc gcaggcggct gttttccagc agcgcgggta gatccaggta ttcggcccag
    18901 agccgcgtgc gttcggggat gagcagcgga tacaccacgt tcgccagcat ttcctgcacg
    18961 cgcgtctcgc ccaccagggc caggggtgcc gcggcgggcg cggatgtcaa agtgtagtgc
    19021 gcggaccagt ggtcgtgccg cagcgcggtc atcgtctcgc gccaggcgtc acggctccag
    19081 cgtcccgcgt ccatgagagg acccgccacc cgttcccacg acgcaagcat cgccgccaag
    19141 gcgcccaggc gtcgctgtgg atgattcccg ggacgcgtcg ccgaaagacg ccagcgcggc
    19201 agatgccggg gttggagcca gcgggaagcc tcgtcataat gcttccacca ctccgaccaa
    19261 agcccgctca aatactcgcg cgagcgcccc gctgtctcct cgtgacggac agcctccatg
    19321 aatcctgcca caccgaacag cagggcctcg cgctgcatgg gcggcagctt cagcatccgc
    19381 ttgagcggca gcctctgcgc cagcaggaca aaaggctgcg ggttcttttg gtaacccagc
    19441 gtgcgcgcca gctcctggta aatcgtctgc gcgcggccct gggcctccgc gcagcggtgg
    19501 atgcggcggc ttttgttctc cagacgatgc tgcgccgcag cctccagcag ggaagctact
    19561 cgcgccgccc ccatctccgc cagcggcgtg tggcagcggc cgagacgcgc cgccgccagc
    19621 ccgcgattcg gaagcgccga gccggccaga ttctccatgg gcagccgtgc ctgcgccacc
    19681 gcgcgatgct cgtgcgtgcg cgtgtagaac cgctccttcg gcgcgtggac aaagagatgc
    19741 agcaccacac ggccgaactc gggattagcg ccatgcccgt ggcgctccca gtcgcgcgcg
    19801 tccatgtcca gttcgatgtc tccgcgcagc aactgcccgt ccacctcgac ggcggtgttc
    19861 gtgaaatccg gccccgctcc cgagttccac tcgccaaagt ccgccatgcg caccctgcgc
    19921 ccctccgggt ccgtgaactc cgcgccgaaa gcgccgccga accagaggct ctgcacatcc
    19981 atctccgaca tggacgcctc cagcccagac aggcacgccg ccggtccctc ctccagtccg
    20041 gcaaacaccg agtccctgaa acgcgcgtag cgctccgcgt gcggcagcgg ggtgaggctc
    20101 ataatgggca cttagctgtt ggacatgcgc agggtcaagt cccgctccac ggcgtgttgg
    20161 ccaggcccgg tcaacccggg cggtcaaggg cgtcattatg aaagtctggc ggtggcagcc
    20221 ggttcaacta cacttgccca tgcccgcgaa gattcccgcc gccgaatccc cggcgcccac
    20281 gcacgatgac ctcatcaaaa agctgctcca ccagcccagg ctgctgcggg atttcatccg
    20341 cgccttcctg ccggagctgg caaagttcgc cgacctcgac cacatcgaat acctcgacaa
    20401 agagcacccg cgcggctcca agcgcccccg ccgccttggc gatgtgcttt tcaaaaccct
    20461 ctggaaaaag tgcgagaccg ccttcctcat ccacgtcgag agccagaaca aaccccaccc
    20521 tgccgcgctg gagcgcatgg gcgagtatgc actgcgcgac ggcatccgct accgcctgcc
    20581 cgtcatgcct gtgctcttgc tcacctaccc gaagccggag accccgcagc ccggcgaact
    20641 gcgctgggac ttcggcagcc tggccgccat ccatgtgaaa tgcccagtgc tgcacttccg
    20701 ccgcatgaat ccgctgccgc atctgcggag caaaaacgtg gccgcgctgg ccatgacagc
    20761 gctgatgaag ctggaggcgg accagcaagt ggacgccatc gtgagcacga tggccgaggc
    20821 gctgaggcag gagttcgagg cggaggacct ggacgcggcg ctggactttg tgaaccacta
    20881 cctgccgctg gcggaggaac aggtcttgca actggaccgg aaggtggata gtcttgcgcg
    20941 caaggaacca aagctcaaac ccatgcccaa gcttatcaac cccttcgtcg aaatcggccg
    21001 gttgcgcgga cgcaaggaag gccgcgtgga aggccgcgtg gaaggccgtg tggaaggtct
    21061 tttcgaaggg cgttccggcc tggcattgcg actgcttgcg cggaagttcc ctggaatcgc
    21121 gcggcgcgcg gggccgttgg tgaaacggct ggacgcggag cagttgctgg cctttggcga
    21181 ggagttgctc ttcatgcaaa ctccggccga gtgcatggac tggctcaagc gcgggacggg
    21241 caagtattga ccgatttttg cggcagcccc agcttttccg cagtcatcca cgcctgattt
    21301 cgcggtcatt gacccagctc gcgcagcgtc acttcagcca gagactccac acggcgcagc
    21361 gcgccggtga agtcgagatg ccgcgtcatc ggtccggggg ccaccacgca gcggatgccg
    21421 gcggccaggg cagcgcgcag accattgagc gaatcctcga aaatgacggc ctcctcaggg
    21481 gacacgccga gcttttccgc cgcatggaga aagagcgaag ggtccggctt caccctgccc
    21541 gtgtcatcca cggtggagat gtggtcaaaa tgcgcgccga gtccaagctg gtccatccaa
    21601 gagtctatcc accagcgcgg gctgctggag gcgatggcgg tggtcattcc caacgcgcgc
    21661 gcctcatgca ggcggtccac cacgccgggc agagtctgca aagtctgccg cagctccgtc
    21721 tcgcgggtgc ggcgtccttc ctcgatttca tcccagtcaa agaccaggcc ggtcaactcc
    21781 tccaggtcgc ggcgcggatc gtagctggtg ttgaagtccg tgccgacgac ctgcgcgtac
    21841 cgctccaccg gcagttcgtg tccgtggcgg gcaaagattt ccttccaggt gcggtaaccg
    21901 gtgctttcgg tgtcaacgat gaggccgtcg aagtcgaaga tcacggcgcg gatgggagag
    21961 gacatgcgca ccgatgcacg ctcgcggtga aagatgcaaa agttgtgaaa gcagggggat
    22021 gtgatttttt gcggtttcgg cttcctttgg gagcgcttcg cgccagcttt cactcatgtt
    22081 ccacctgagc aaacccgcgc cggatgatct ggccgcgcgt ctttccgaat ggcggtccga
    22141 acccctgagc tacaaacacc agggcggcat cggcatcccg ccggaaacag gcttcatcgt
    22201 ggatgcccac gaggtcttgc tcgggcgcgg ggagtctgtc tttcaatcag cgggtgccgc
    22261 gctggatgag tggaagatgt tccccgcctg ggcaggcgtg gcgcgcgttc gcgcctccgg
    22321 ccaggagccg ggggagattg tggccatgat cgcgcgcatc tgcggcctgt ggtgggtaaa
    22381 tccctgccgc attctgcgcc ggtgcgacgg cccgcgcagc cacggcttcg tctatggcac
    22441 gctgccggag cacgccgagt gcggggagga gcagtttgtt attgaatggc gcgccgatga
    22501 cagcgtgtgg tacgtcatcc gcgccttctc ccgtccgcgc cactggatgg cctgggccgc
    22561 cttcccactg gcgcgctggt ggcagtgccg ttttgtgcgg gactcacagg cgaggatgaa
    22621 aggagctgtt tcatgagaat ctggtttcag gcgctgcttt ggctcgtgat gattggcttg
    22681 cagtggaggg tctgccagac ttgcgcggcg ggttcttgct ccgtgggcat ggttgatcca
    22741 ggtggtccga tctggcatgt gaatgcgaat caggcgcaca tgctcattca tgcgctgctt
    22801 ctattttcgc caatggtcct gatgcctctg ggacgaagat tgttcggcgg ctcctcgtcc
    22861 ggccagacac cctggattga aattttcgcc gccctttgcc tcggcttgtc actgctgttc
    22921 aggccggttg atccgcgggt cgtgtttgag gacgattcga tttcacggag agtcgtgccc
    22981 gccgggttct tccttgcgct gccctggctg gcggtgcgtt cgtggagtgg cgtggtcgtg
    23041 ctccgccgct ggcggcatga ggggttcgga gggctggggc atgcttgtgt ggctttggcg
    23101 caggtgttcc ccgccattgg ggcggcatgg ctcgtggctc atcgcgcgaa ctggacgccg
    23161 tggaacttcg acccgctcat cgtgctgctg acggcggcgc atttccatca tgcgggcttc
    23221 acattgcctt tgatcgccgg attgaacgcc agggccagtc cgggccggct gacctcagcc
    23281 tcatgcgcgg cggttctcgc cggcgtgccg ttggtggcgg ctggcatcac ctgcgcgcat
    23341 ttcgggatgc tgccgctgct ggagccgctg ggagtgatgg tgctggtggc cggcgccatg
    23401 ggagtggctg cctcgcagtt gctgacgggg gttgcgtctc gtggaggaat gcgctggcgg
    23461 acacgcgcgg ccttcctgtt ttcaggcgcg tgtctgatcg tggccatgat cctcgcgcta
    23521 agctttggtc ttcgttacct gtttccgcgg cttgctttga ccatgccgca gatgtggatg
    23581 atccacggct cgctgaacac tttcggtttt ggcctctgcg gcattctggc ttggaggtcg
    23641 ctggcgggac ggggtcaaag cgcgggctga ggcgccgggg ctgcttcctc cactctgccg
    23701 cgtcccgcgc gccaggccag caatgtctcc gcgccaagca ccgcgagcaa tgccgccaga
    23761 aaccagagcc aggcacgctg ctgcttctcc gcctcaccag cggccaggcg ctcctggaca
    23821 cgctcgtttt gggcgatctc cccctgcgtg ccgctcatct catgcaagcg tatgccaaag
    23881 tccttgaggc gctccgcatc cattgctgtc acgcggccct cctccggagg caggttcact
    23941 gcgtggaggc gtgtttcagc tcccgttttg acttggtaaa tcccgacgga ttcggtgacg
    24001 taaggcatgc cagctttgac cgttgtttcc ctgccctccg gcgtgagcac gagggcgtca
    24061 ccggctgtga ccggtagtgc ggcgccgacg agcatgcccg actgggcggc atgggaaaag
    24121 cccgccgcct ccagccagcc gaagatcagc ggcacgaact tcgtggagag cgcgagctgg
    24181 ctgtccgccg gatgccagcc gctggccagc atgagcagcg tgcccttttc catttgcaag
    24241 gccatcaacg cgggcgcgcc gttgtcaaaa cgggcgatga tttcgatctt ctccgcagcg
    24301 gtgcctgtga ggtcggcctg gcggtgtttc caaaagcgca gcttggtgaa atcgcgcagc
    24361 cgcggatcgg cgaaggggcg cagcagcgga tgctccgctt tgacctcgcc gagcatcctg
    24421 tagttgtcca cgggagcttc tttcaatgcc acgccattca ctccggccaa agactccaca
    24481 tcctgcgcgc tcgttcctgg ctccagaagc catacagcca gccctccagc gcgaatccat
    24541 tcagcgaatc cctcgggtgg ggcgccttgc agcacggcca tctggcagcg cggcagctcc
    24601 gccgcgtctg ccacctgcgg ctccaggctg gtggtcggct gcaaggcgcg cttgagataa
    24661 aagagcggcg atgcagcctc gtccgccgcc gccttcgcgc ctagccaggc cacgcccacc
    24721 tggcgtggcc tggcgggggc caggaagatc ctgttgtcaa agtcccacga gtcaccgctg
    24781 agcaaaagcg tgtcagccga agcggcggcc tgggtgggca gcggcacact tagaatacgc
    24841 gcggctcctg cgggcaggta accagtcatt gcctcgcccc tgctcttttc ccaccgcagg
    24901 gtgaagtcgg aaacccgcga gtcccgtgag tttgacaggc gcacgcgcag ggtctccgcc
    24961 tctcctgttt tagcgctccg gtcctccaca tttcccgtat ccgccgctgc gagactcaga
    25021 gtcatgttgc cctcgtctct cgtctcaacc cgcagcgcct gcacgcgcgt ctcctccggc
    25081 caggcaatgc cgcgcagggc gtcgagtctg gcgccttcct ggaagtcggt aagcaccacg
    25141 atcacgcgct ccatgccgcc gatgccgctg gcgcttgaga tttgcgccag cgcgtccaca
    25201 agcgctcggc ccaagtcggt ggcctgccag ccgggctgga tttcaccgag tttctggagc
    25261 agcgcacctg agcgtgccgg gccggattcc gcgtcttctc caaaggtcca cagcggatgc
    25321 agccggtcgt caaatgccgc cagtgccatt cggtcggcgg gcgtggtttt ttggatcacc
    25381 tcacgcgcgc ggccgagagc ctgcttcatt aaatccccgc gtcgcattga agcgctgcgg
    25441 tccacaagca tgaagacggc cctgcccccg tctgctccgg cgcgcgcgtc agtctgaagc
    25501 cagggccgtg agaacatcag cgccagtagg atgagcgcta ggcagcgcag cagcagcagc
    25561 agccagtgct cgatgcggcg tttgctgaca ggcacgggcg tccgcgcctg gagaaacatc
    25621 agcgagctga actccacccg gtcctgcggc ggcctgcgcc gcagatgcat gaggatcgga
    25681 gcaatgatcg cgcccgcgcc gagcaggtag agtgggaaca gccaggacat ggggaaaatg
    25741 ggctttcagt tgaaccctcc gaaggacgga atcttgcatc cgcaaggcgg gaagaaaaaa
    25801 tggaggaccg tcctggctgc ctgcccggcg gctggggggg ggacgggcct ggatgacacg
    25861 caactccatc ttggcggggg ttcggaagcg cttcctgcca tttacgcaag ttcctccagg
    25921 agcgcgcatc cagtctgcaa ggggccgccc gcctcatgaa cccgcagaca agcccgccat
    25981 gaaactcgct tttcaaatca tccttccagg tctcctctcc ctctccgccg ccgcgcagga
    26041 tgtccgctgg acgggtgctt tgagtgagtt ttgggacaac tcccgcaact gggcccgggc
    26101 caccccgccc aactctgacg gggatgtggt tttccccgcc aatgcgttgc gcaaggcatg
    26161 tcaaaacaat ctcccggcag ggactgcgct gagcaagctg gtcttcgatg ccggcggata
    26221 tgtgatcgag ggaaatccgc tgcgcatcaa atccggcggc atcatcctgg ctgacggaac
    26281 agcggccgaa cgcgtggtgt cgctgcgcct gccgctgact ttgggagcct cgcagacatt
    26341 ctccataggc ggcctggcag ggagcgcggg tggtctgctg ctgcatttga agggcgcggt
    26401 gagcatggga gatttccctc tgcaaatcga gggcggggac gcggtgctcg acgcgccggt
    26461 cacagccaac atgcgaggcg gcaccgcgtt gaaattcaca ggcatcggtg tgaaaaccat
    26521 atcgcagggc gtgacaatca ccgctcccag gattgaggtt tcagggttgg ccggacatgt
    26581 gcgtctgctt ggagaggtgg tggctgacac catgctggtg accgccctgc tgccgcaaac
    26641 accgggcaag ggcggcgtgc tttccgggac aggcagagtg gaggcggact gccgggttct
    26701 gcgctatggc gtcctgtctc cgggagtgga aggttttggc cggctgcgca tggataagaa
    26761 tcttgttcta ggcggagatt ccctggtgga gattgagctg gggaatccgt cctccgccgg
    26821 gaagactcat gaccaggtgc gcgtccaggg cgcggtggaa attcaaaccg gcgccagcct
    26881 gctcatcaag cccggtgccg gcttcgcccc ggtggcggga caggcctata ccgtgctgga
    26941 aaagacgggc gcgggacccg tcacaggccg tttccagaat ctaccccagg gcgccacaat
    27001 cagagtgggc gaggccacca tgctggtctc ctacacaggc ggtgatggca atgatgtgac
    27061 tgtcacggtc gtggacctgc ccccggcgat ctctgccata cccgcgcaaa ccgtgcatga
    27121 ggacggcaga gtgactgtgg ccttcaccgt gtcggacccg aacaccgacc tctccgctct
    27181 gcgagtcagc ctgcgaggcg ggcaagtgac ggcatccttc tcgggtgcgg gcgcggccag
    27241 gaccgcgacg atccgtccgc agcctgactt tttcggccaa accactgtca cacttgaggt
    27301 cagcgatgcg acagtctcca cgcgcaggag tttcgagctg gaggtccttc cagtgaatga
    27361 cacgccgtcc ttcaccgccg cccctgtgat cgagttcgtg cgcggcgctt tgcagtccag
    27421 ggcggcggct tcaaacatca gcgcgggatc gggtgaggag caggcgctgg cattcgaggt
    27481 gctttcagtt tccaatccgc aactgttccg cgtgcttcca gcgctgacgc cggatggcat
    27541 gctcacctgc ggccctgtgg agaccggcga atcattggtc cgtgtgcgtc tgctggatga
    27601 tggcggcaac gatcatggag gcatccatgc cagcccgccc cagaccatcc tgctaagagt
    27661 aggcgcggac aaagaatcac cgattgcccc gcctgtggat gtgacatgga ccggtcttgc
    27721 cggcagccag tggaacaacg gacggaattg gagcacgctt cttgtcccgc aacatgccgc
    27781 taccgctgga tttgcggcag acctgccggc gcggcgcagc aagtccgtgg acatttccgg
    27841 gggcgcgcag tgcgggcagc tgcgtttctc aggaggcgga taccgcctgc acggcggaga
    27901 tggcgatttc atcctggccg cggaggcccg gctcacagcg ggcgtggaca cgaggtttga
    27961 cgctctgctg aaaggccgcg agagcttgag tctcatcgcg ggccagggag caaggctgga
    28021 gttgaaaggc ggcctggagt gccctttcag gggctttgcc ttaccaatac cgcctgtgag
    28081 gccaaacaaa ctttttatga acctggggtt cggctcggtg atggagatca acggcgggcg
    28141 tctggcggat gtggatatcc tcatgggcgg caagggaagg atcgtcatgg agggtgatgt
    28201 tttccacatg ggcgaaacga atctgttcaa cggaggattt catgtgcttg aggtcaatgg
    28261 cagctttggc acgcggttga cccatgtgga gagggatctc aactccgtgg tttttcaaag
    28321 cacggccacc ctgcgtggaa cagggacagt gcttggcagg ctcagcgccg gcggtctcat
    28381 ccagccggga cggacaggcg gcgtggaagg ggacgccgcc ggtcaaatcg gacgcctgac
    28441 cgtgcatatc gccaccgtgg ttgacaacgg cgcggaaaat ctcggcgtgc tcgaactgga
    28501 catcggctct gccgccggtc ccggtgtcac ctacgaccag cttatcgttg aaaagaggct
    28561 cgttctggac aacgcccgcc tgcggctgcg cccggctcag ggattcgcac ccagggtggg
    28621 agatgtgttc acactggtga atgtggccgg ccccgtgacg ggcgcgcgca cgggcttttt
    28681 ttccgggttg cctgagggcg ccacgctgcg ggtcgggaat gcggacctgc gcgtgagcta
    28741 cacgggaggc gatggcaatg acatcaccgc cacagtgacg gcactggcgg caggtgcgag
    28801 cggcttcgcg tccgcctcaa ccctcacaaa tgccgccacc ttcacacccg gctcttactg
    28861 cggcctggct gtggctgatc agggcgtgct gccgggtcat gaaaacgccg ggttactctc
    28921 gctgacggtg acccagaccg gcacgttcag cggcaagctg ttgatgggtg gcgccagcca
    28981 caagttctca ggcgccttca agagtgatgg cagcgcgctc ttcggcaaag cgggcggcct
    29041 gctgactctg ccgcgcaaaa acctcgcgcc cctcgaactc tccctttacg cggacagttc
    29101 cgagggcgcg ccatccgtca ccgcgctcat cctggaagat ggccggccct ttgccagcgc
    29161 gcgcgcggac cgcgcccttt acgattcggt gaaatccccc gcgcccgcgt ggatgacgga
    29221 tgccaaaagc ggcaaaggca aattcaccgc agctttcccc gcgctcgccg caccgaaccg
    29281 cggcctggag gccgcctcct tcccgcaggg cgatggatgg gcgctgatga gtctcgcgcg
    29341 ggatggcagg gtgacgatga caggtcaact ggccgatggc gcgcagatca ccctttccgg
    29401 cctcatcacc accggcggca gtctctccat ccatgtgccg ctttactccg gcaaaggctc
    29461 cctttccggc atggcacgtt tcgagcagca gcctctcaca gacgcggcgg gggtggactt
    29521 tttctggttc cgccccgccc agacgccgcg cgcagcagcg ccctatgcgg cgggctggcc
    29581 gcaaggaatc catgtggatt tcgacgcctg ccgacttgtg gaaaaagccg ccccctatct
    29641 cgccggtctc gctgctccag gaccggctgg caatgtgctc atcacagtgg acggtctggc
    29701 gcaaaaggct gccagcctgg aacccgcgaa caaaatcgtg ctcgtcaatc gccccgaccc
    29761 tgacaagctc gccagcgcca tcaccacctc ctccggcaaa tggagcggct ccttcatcca
    29821 tcccgccgac atgaaaaaga aagccttcag cggtgtggtc ctgcaaaaat ccggacgcgc
    29881 caccggtttt tacctcgctc ctcaaggcag cggcctggcg ggtttttcgg ttgttccctg
    29941 agttgagcgc ggcccggatg gttgacggac tgccatgttc ttcccgcttg ggtggagtcg
    30001 cggggcgcac ggacaaaatc caatctccca tcgtgtgcaa cggcgcgcgc ctggaaagcc
    30061 tcgccgttcg gagagaagcc ctgtttttga tcgagattcc gccggggaag cgtatcctcc
    30121 cccgaatttg cagcactcca tgtcccatca cgcccccaat cttgaccacc atttcctcag
    30181 ccgccgccag cttttgagcc gggtgggcat ggggtttggc gccgtggccc tgggcgagat
    30241 gcttggcgcg ggcaaggcgc atggcgctct ggaccagcgc agcccatttg ccgcgcggcg
    30301 gccgcatttc gagccgaagg cgaagcgtgt cattcacttc ttcatgaatg gaggcccctc
    30361 gcaggtggac acctttgacc cgaagcccat gctggacaaa tacgacggca agccgctgcc
    30421 gaacctgctc aagaccgaac ggcccaccgg agctggcatg aggtcgcctt tccagttcaa
    30481 gaaatacggc cagtgcggcc tggaggtcag tgatattttc tcccgcaccg ccgccttcgc
    30541 cgacgacctc tgcgtcattc gctccatgca ggccgacgtg ccgaaccacg agccctccct
    30601 cggcctgatg aactgcgggg aaagccgcct gcaccgtccc agcatggggt cctggatcac
    30661 ctacggactc ggctccgaaa atcagaacct gcccggctac atctccatgt gccccaaggg
    30721 aatgccgacc cgccgcacca aaaactggca gtccgccttc ctccccagca tctaccaggg
    30781 cacctacatc aacacggagg acaccgaggt ggacaagctc gtcgagttca tcaaaaacag
    30841 ctcgctcgac ctcaaacagc agcgccgcca gctcgacctc ctccacgccc tcaacgagca
    30901 gcacctccgc cagcgcgaca acgaccagca gctagacgcc cgaatccaga gctacgagct
    30961 ggcctaccgc atgcaaatgg aggcctcgga cgctttcgac atcggcaaag agccagagta
    31021 tgttcgccag gcctacggtg aaaacgactt cggccgccag accctcattg cccgccgcct
    31081 tttggagcgc ggcgtgcgct tcatccagct ctggaccggc gccggccagc cctgggacag
    31141 ccacgatgag atcctcgacc accagcgcct ggccaaagac gtggaccagg ccatcgccgc
    31201 cttcatgcac gacatgaagc agcgcggcct ctttgacgac accctcatgc tctggggagg
    31261 cgaattcggc cgcacaccct ccgtggagct gcccaccccg ggggccaacg cgggcaagca
    31321 gcgcgggcgc gaccacaacc actacggctt cacctgctgg atggccggcg gcggcgcgcg
    31381 tggaggctac actcatggcg ccaccgacga gttcggcttc caggccgtgg agaatccggt
    31441 ccatgtccac gacttccacg ccacgctgct gcgcctgctc ggtttcgacc acgagcgctt
    31501 cacctaccgc tacgccggac tcgacttcaa gctcaccggc gtcaacgact gcaaagtggt
    31561 gagggaactg ctggcctgat ccttttctca tttgcgcggg ctggcattgg actcgccaag
    31621 cctcaacatc gggccatgcg gcgcgcatcc cgccacccgt acccgtcagg acgctcaaac
    31681 gcgctcatga actggggcct cgcagttgcg gctggatttt gtgaagggtg cgcgggcctt
    31741 ttagcaagtg gggctgacga tggcgaggcc attgagcagt ctcatgcaga tgtaactgtg
    31801 ggctgggagg gcaaagatgc gcgcgccgtg gtggcgcgcg gggtgcctgg gggggtggtc
    31861 tacggtggtg tcgaagagta tctcccagag ggtggctttg tctccgggca ggaggaaatc
    31921 ctggtcatgg tttccccgat taaataggag cagcaggggc ggctgggagc cgagcagcgc
    31981 gccgaagtgg tagcgccccg gctcgtgcca ttcctcatgg caaagcaggg tgccgtttcc
    32041 ctccagccag aggatgtcgg cgaggccggt ggcggggtcg gtctggccgt caaaaaaggt
    32101 ggcgcggcgc agggcgggtg tggcgcggcg cagggcgatg atgcggcggg tgaaggcgag
    32161 cagcgcctcg tcacaggatg accagtcgag ccaggtgagg tcgctgtcgt ggcagtaggc
    32221 gttgttgttg ccgccttggg tgcgcccgcg ctcgtcgccg gcggtgagga agggcacgcc
    32281 gacggaggtg aaaagggtgg cgagcaggct gcgcttcagg cgggcgcggc gctggagaat
    32341 ggcggggtcg tcggtggggc cttcgacgcc gcaattggtg ctgtggttgc tgctgtcgcc
    32401 gtcgcggttg tcctcgccgt tcgcctcgtt gtgcttgcgg gagtagctga cgaggtcggc
    32461 gagagtgaag ccgtcgtggg aggtgatgaa attcacgctg ctgagggcgg gccgctggct
    32521 ccagccgtac acgtcctggc tgccgcagaa acgcttggcg aaactggcgg tggagccggg
    32581 gctgttcgtc cagaaacgcc gcacgctgtc gcggtatttg ccattgagct cgcgccaggg
    32641 ttcggggaag gcgccgacct ggtagctgtc catgcgcatg atgtcccagg gctcggcgat
    32701 gagcttcacc tgggaaagga cgggatcctg ggcgacggcg gcgaggaact ggctgttgga
    32761 gtcgaatgcg tcacgcccgt cgcgggcgac ggtgacggcg agatcgaagc ggaaaccgtc
    32821 cacatgcatc tcggtgaccc agtagcgtag gctgtcgagg atgaggcgga gggccggggg
    32881 gctggcggag ttcacggcat tgccgcagcc ggtgatgttg atgtagctgg cgccgtgctc
    32941 gtgaaagctg tgccggtagt agaggcggtc gtccaggccg cggaacatga gcgtggggcc
    33001 gcgctcgtcg ccctcggcgg tgtggttgta gacgacatcg aggatgacct caagaccggc
    33061 gtcgtgaaga gcgcggacca tttgtttgaa ctcgcgcacg cagccttcgg ggtcgcgcgc
    33121 ggcggcgtac tcgttgtgcg gggcaaggaa gccgatggtg ttgtagcccc agtagttggt
    33181 gaggcctttt tcgagcagga attggtcgtc caggtgttgg tggacgggca ggagctggag
    33241 gctggtgacg ccgaggtcct tcaggtagcg gatgacctcc gggcgggcca gtccggcgta
    33301 ggtgccgcgc agacctgcgg gcacgcgcgg atgggtgcgg gtgaagcctt tgacgtgcag
    33361 ttcgtagagc acggtgtccg cccaggggat gcgcgggcgc tcgtcgcccc gccagtcgaa
    33421 gtcgccggcg atgacggagg atttcaaggc ggtggggccg ttgtccacgg agcccggctc
    33481 ggttcccgga ccgtgggtgt tgagcatgcc ggtgcgtccg tccggaacac cggcaatggc
    33541 gagcgcgtac ggatccagca ggagcttgtt cggattgtag cgcagcgcct tgtcaggcat
    33601 ccacgggccg tgggcgcggt atccgtaaag ggtgccgggg cgcgcgcctt tgacaaagac
    33661 gctccacagg tcgcgctcgc cgcgggtcat ggagtgccgc ccggtctcag tgtccggggc
    33721 gaagggtgag tagaggcaaa gctcgaccgc cgtggcatgc cgggagaaga ccgtgaagtg
    33781 cgcgccgttt ttggtcagcg tcactccggg gggcagggga tcgggaggga tggttttttc
    33841 ctgggagaaa agcacggctc cgtctagcgc agggatcggg ccggggcgag aagggaattt
    33901 gagccgctga ggcccggaag gcggcggcat gtgtttccgg tctttgccaa accgcgtatc
    33961 cgttttattc tccgcgcccc atgactgccg ccaccatcct cccgcttcct gtgctgccag
    34021 acgcccacgg ccacttcggc aaatacggcg gcatgtttgt ccccgagacg ttgatgtcgg
    34081 ctctcaccga actatccgac cattacgaga aggccaaggc ggacccggct ttccaaaaag
    34141 agctcgacgg cctgctgcac gagtatgtcg gccgccccac gccgctttac ttcgccgagc
    34201 gctggactga aaagctcggc ggcgcgagga tctacctcaa acgagaggac ttgcttcaca
    34261 ccggcgccca caagatcaac aacgcgctcg gccagatcct gctggcgaag cgccttggca
    34321 aaaaacgcat catcgcagaa accggcgccg gccagcacgg cgtggccacg gccacggtgt
    34381 gcgcgcgctt tggcttcgac tgcaccattt acatgggcaa agtggacatg gagcgccagg
    34441 cgctgaacgt ggctcgcatg agatttctcg gtgcgagggt cgtggccgtc accgccggcc
    34501 aggcgaccct caaggaagct gtgaacgagg ccatgcgcga ctgggtgacc aatgtgcgca
    34561 ccacgcacta catcctcggc tccgcgctcg gatcgcatcc gttcccgatg atggtgcgcg
    34621 acttccatcg cgtgatcggc gtggaggcgc gccggcagat tctggagcgc gagggccgcc
    34681 tgcccgacct gctcgtggcc tgcgtgggcg gtggcagcaa ttccatcggc cttttccatc
    34741 cgttcttgaa tgacgagggc gtgcgcatga ccggcgtgga ggccggcgga gacggcatca
    34801 tccccgaacg ccatgccgcc cgcttccagg gcggccgtct cggcgtgctc caaggcacga
    34861 agacctggct ccttgccaat gccgacggcc agatcgagct gacccacagc gtcagcgccg
    34921 gcctcgacta cgccgccatc ggccccgagc acgcatggct gcacgatcag gggcgcgtga
    34981 cctacaacta cgcaaccgac gacgaggcgc tcgccgcctt ccaggagttg agcgcggtgg
    35041 agggcatcat cccggctctc gaaagctccc acgccgccgc cgaggtccgc aaagtggcac
    35101 ccacgatgcg cagggatcag atcatcctgg tcaaccttag cggccgtggc gacaaggatg
    35161 tggcccaagc cgcgcgcatg atctttggcg aggagttgaa gttctgagcg ggcgcgacgg
    35221 gtgaaatacg ctccatcaag gagacgttcc cgccgcagcc atgctccgtc ccatcctgct
    35281 ttccctcctc gccaccgcgg cccacgctca aaccgctgcc gatttgctca ctcaatctgg
    35341 cgtcaaaggc ggccttgtcg tccatgttgg cgcctccgat gccaaaatca ccgccggctt
    35401 gcgtgccaat gactccttcc aagtccgcgc cctcacccgc gatgccgcca cgctggagaa
    35461 gcttcgcgcg ggcatcggag cggattacgg cgtggtgtcg gcggacctgc tgagcacgcc
    35521 gcatctgccg ttcatcgaga acttggtgaa cttgctcgtg gtcgaggatg cgcaggtgac
    35581 gcgcgaggag attgaccgcg tgctggtgcc gaacggcgtg gctttgatca agaaggacgg
    35641 cggctgggag aagatcgtca aaccgcgtcc tgctgagatg gacgactgga cgcactacgc
    35701 ctacgacagc aaaggcaacc tgacctctaa agacatgctg gtcggtccgc cgaagcgcat
    35761 gcagtgggtg ggcaatccgc gctggtcgcg ccaccatgac cggatgtcgt cggtgtcggc
    35821 gaaggtgtcc gagggcggcc gcattttcta catcatggac gagggcagcc gcatctccat
    35881 cctggtgccg ccgaagtgga tgctcatcgc gcgcgacgcc ttcaacggta cggtgctgtg
    35941 gaaaaaggag atccccgaat ggagcacgca cctgtggccg ttgaagtccg gccccaccca
    36001 gctcacgcgt cgtctcgtgg cggtggatgg caaggtgtac gtcacgctcg gcatcaccgc
    36061 cgccatcacc tgcctggacg gtgccaccgg cgaggtggtg cgcgagtatc ctgagacgaa
    36121 aggcacggag gagatcctcg tccgcaacgg cacgctttac gcgctggtga accccgacgc
    36181 tgaccggctg aacaaagagt tcgccgtggc cgcgcagagc gatcagaagc gcgtggagac
    36241 ggagttcaac tgggacggca agccgcgcat cctgcacgcc atcgcggcgg actcgggcaa
    36301 ggtgctgtgg aaaaaagaag accgcatcgc accgatcacg ctcagcctgg atgacaaacg
    36361 cgcggtgtat tacaatggcg acggcctcgc ctgcctcgac atcaagaccg gtgagacgaa
    36421 gtttgccgac acccccacgc agcgccgcgc cttgtatgag ttcaacttcg ccccgcgcgt
    36481 cctgctgcac gatgatgtga tcctttacgc cggcggtgac ggcagccaga aggcggtgaa
    36541 cgcggacacg ggcaaggacc tctgggtggc cccgcatgag aagagcggct accgcagtcc
    36601 cgaggacctc atcgtggcgc agggtctggt gtggaatgcg ggcacactca gcggcaatca
    36661 aaccggcgag tacacgggcc gcgatgtttt gactggcgag gtgaagaagc aattttatcc
    36721 cgacgtgccg gagggcactt actggttcca tcaccgctgc tatcaggcca aggcgacgga
    36781 gaagttcatc atcccgagcc gcaccggcat tgagtatgtg gacatggaaa aggagcactg
    36841 ggacctgcat cattgggtgc gcggtgcctg tctctacggc gtgctgcccg ccaatggtct
    36901 cacctatgcc ggaccgcaca actgcgcctg ttaccccgaa gcgaagcttg atggcatggc
    36961 cgtcatggcc agcaccgcgc gttacccgct gcccgcgcac acgccggacg atcagcgcct
    37021 cagcaaaggc ccggcctttg gccaagctat cgaggaaaag gacgcggccg ctggggattg
    37081 gcccacctat cgcgccgaca acgcccgcag cggtcatgcc aaggccgacc tgcgcaagga
    37141 tatgtcgctg gcctgggaag tgaagctcac gccaccgctg agcaccacga ccatcgccgc
    37201 cggtctgacc tttgtctccg aagtggacaa gcacacgctg cacgccatcg agaccgccac
    37261 gggcaagacc gcgtggcgct tcgtcgccgg gggccgcatt gactcgccgc cgacgtattg
    37321 gaaaggccgc gtgttctttg gcggcaagga cggctttgtc tattgcctgc gcgctgcgga
    37381 tggggcgctc gtttggaaat tccaagccgc gccttcccag ctcacgcatt gcgcctggga
    37441 gatgatcgag tccgtctggc ccgtgcatgg cagcgtgctg gtggaaaacg acctcgtctc
    37501 cttcgtcgcc ggtcgctcct gcttcctgga tgaagggctg tggttctacc gcctcgacgc
    37561 ccgcactggt gccatccgag tcaaagagca ctacgacgac cgcgaccccg aaaccggcgg
    37621 cccgctcaac gaccgccaca agaccctgca aatgcccgtg gcctccaacg acatcctcag
    37681 cagcgatggc aagtggacct tcctgcgcac gcagaagatc ggcgccgatg gcaagcgcgt
    37741 ggagatcggc ccggtgagcg gcgacttcgc caaacaaggc ggcgcgcaca aaggcgaggg
    37801 ccagcatctt ttcgcgccga tgggtttcct ggatgactcg aacttccacc gcagctactg
    37861 ggtctatggc aagagcttcg ccggcggcca cggcggttat ttccaggccg gcaaatacgc
    37921 gcccgccggc cgcgtgctgg tgcatgacga caagaacgtc tattcgtatg gtcgcgaggc
    37981 caagtactac aagtggacca ccaccatgga gtacacgctc ttttccacac cgaaggacgc
    38041 cgccgaggag gcctacacga aagaggacgc gctcaccccg cgcaccggca acagcgtcgt
    38101 gctcggcccc gatggcaagc cgctcgaccc tcagccgccg aagctcgctg tcgaaggcaa
    38161 ggccgcgaag ggcaaaggca agggcaaggc gaaggcagcg cccgccaaag gccccgcgcc
    38221 gcttgcgggc tctgtgcgtt tcccgagcaa cgccaccacc gatcccaccg gcaaaggtct
    38281 cacgattgaa atgtgggtgc ttcccgatca ggcgaacggc atgctcgtga acttcggcgg
    38341 tccgcttgtc ggcttcgccc tcgagcttca gcaaaagaag cccagcttcc atgtccgctc
    38401 tgaggcaaaa ctcagcagca tcgccgccaa agaagcgctc ggcgacggtt ggacgcacct
    38461 caccgccacg ctctccgagg acaagaccat gcgcctttac gtcaacgccc tgcaagtcgc
    38521 cgaaggtcag gccagcggcc tgctcgacaa gatgcccgcg cgccccttcc tgcttggcaa
    38581 cagcgacggc ggcgcttccg acagcaaggt cggcggttac agcggcctac tggaccagtt
    38641 cgcctacttc acccgcgcgc tcagccccgc cgagatcgag gcccgcttca acgcgcccga
    38701 ggtcactccc aaggacgcca ccatcgtgtg caattttgac aacggcgact cgcgcgactc
    38761 ctccggcaac aacctccacg gcatcggctc cggcatggag accggcaagg gcaaggtcgg
    38821 cggcgcgctc tggttcaaaa acagccccgc aggcggcggt ggcgggcagc agcccggcag
    38881 cttcgtcaag cacacctggg accgctttgt ccccatcgtc gcccgcagca tggcgctggc
    38941 cggcaacagc atcctcgttg gcggcccgcc ggacaccatt gatgaagaat acgccttcga
    39001 gcgcctcgcc gcccgcgacc ccgccatcct caaggagctt gaggagcaaa acgaagccct
    39061 cgaaggccgt cgcggcgcca agatgtgggc cgtgaacgtc gaaaccggcg agcaatccac
    39121 cggccttgaa ctcacagccc cgcccgtgtg ggacggcatc accagcgcca acggccaagt
    39181 cttcgtcagc accgtggacg gacgcttgca gtgcttcggg aagtgagacc gtggaagcgg
    39241 tgttccccga cgctttcgcg aatcccccct catcaagcca gccgccaagg tccgcgcatc
    39301 ggacgggacc gcatcgcatc cgcttcctca tcgccgatga cgcgctcggc tgccgggtcc
    39361 cagcgcaggg acttgcgtcc gaggcgcagg gcgatgtttg cgaggtgggc aatggtgatg
    39421 gaacggtggg cgtattcgat gggggcggcg gttttcacgc cgtcctggat ggcatccaca
    39481 aagttgcgaa aatggtcttt gctctcatag agacgcaggt cgccctcttt gagcggcttg
    39541 cgcagctcga cgggttcgat cttcaatcca ccgcgcacgc aggtgatgct gccgagttcg
    39601 ccttcgaagg cgatgcctga gccgaagtcc atttgattcg ccacccgcac cagtgtgccg
    39661 tcagcataac gatactcgaa ggaaaaggcg ggcggtgtgg tgtataggga gccgggatcc
    39721 ggccaggtcg cctgcaaatt ggaaaactcc accggaccgc tttcatcctg tcccagcgcc
    39781 cactggacga tgtcgagatg atgcgcgcca aagtcggtga tgttgccccc tgagtagtcg
    39841 taaaaccacc gccagttcga gtgcaggcgc gcggggcaga aaggcgcgtc cggtgcgggg
    39901 ccgagccaga gatcgtagtt cagtccctcc ggcgccggcg tgggcgaggt ctgggcggca
    39961 tggccgttgt tgtcgcgatt gtcgctggcc aggccgacac ggatttgttt gagcttgcca
    40021 acgcggccgt tgcgcaccag ctcgcaggcc atgcggaaat gcacgtcact gcgctgctgg
    40081 cttccggtct gccagacaat gccggctttg ctgacctcgt cactcatgaa gcgaccttcg
    40141 gcgatggtga ggctcagcgg cttctcgcag tagatgtgct tgcccgcgcg cgcggcggcc
    40201 acggcttgca gcgcgtgcca gtggtcgggc gttgcgatct gcacggcgtc cacatccgcg
    40261 cgctggagca gctcgcggaa gtcctcatga gtggagcagc ctttgccatc gtaagtcttg
    40321 tccaccagac gccgcatcgg ctcgcgacca agggcgccgg cttttttgat gatgcctttg
    40381 ttgtacctgt cggattcgcg ctcgacatca cagacggcga cgacctgcac gcgagggtcg
    40441 ttgaggaaat tcttgaccac atccgtgccg cgcccgccgc agccgatgag gccgagcgtg
    40501 gtgcggttcg agggcgcggg gcgtccgtct ttgccaagcg cggaggccgg aatgatgacg
    40561 ggaaagccga gcgcgccgag gctggcggca gtctggcgga ggaagcggcg gcggttggcg
    40621 cgtgcggcgt tatgcatgac ctgttctcga acgcgccgcg agaccgcgtt ttatacttcc
    40681 cgtcatctca aattccgaaa tcggcagttg gagtcatgcg tgttggagca ttggaaaccc
    40741 agcactccaa gagtccgggt cggcaaaggc tccggatgtt ttgtgactgt cttcaccttc
    40801 tcacaggact ggaatggatg cccgcatggc gtctgacttt cccagggcga tgtagggaca
    40861 gcgatgtagg gacaggcact ccagaaagcc gcctttactc ctcgctgcgc ggcttttctt
    40921 cgctcagatc agtccaccat ttctattgat tcacagtgga ctgcgaaaat catgctcaca
    40981 aaacgcctgg cagctattga gctattggcg atgcatggca gctattgagc tattggcgat
    41041 gcaaggaggg gcacccgtca aaagtttggg cgatgacaag cagcgcagca cctcacaacc
    41101 tcatcgccca aagtttttac ggggaggatg ccttgcaacg agcgcagccg gcacgacgca
    41161 cggccacaac gcccacgaac cacgcttgcg cctcactcga aagacaaaga ggccgagata
    41221 gtgaaggcga ggcacgagcc gcaacggtgc gaagcaatcg aggccgatgc gggcggaggt
    41281 ggtgcggagg cggttggcgt tcggaggcac gagcggagct ggtgccgacg gtgcggagct
    41341 tcaaaagggc ggtgcggctg gtgtggcgga gctgtgcagg gcggcaggtg gcgaagcgcg
    41401 gagggcctgc acagcgtagc cacacgggcc gctgcggtgg gtgcggtttg cgccgggcgg
    41461 cgggagcatg aggcatgaca cgggcggggc tcggggtggt tggccgtggc gtgactcatg
    41521 cgtccagggt ggccggtgcc tgacggccat gcggcctgag cttcttcgcg gcagagagag
    41581 gcacgaacgt agccgcctga taccgggaag gaggtaacta cacacaatgc gctgtagtgt
    41641 ggcgaggctt tgcgagcgca cagttggccg ccgggtcccg ctgctcggag cgggtggcgg
    41701 ccaactgcac actactccgc attgtgttgt ggttaccgca ttgggtgcgg agccggggag
    41761 cttcgcgacc cgcactcgat gcggttggtt ccaacgggag cagagctgcg gcgttacgga
    41821 aagccgcaca cttccttgcg gtgggggttg gagagggcgg agcatccctg cggttggtaa
    41881 cggtgcggag ccgcgagctt tgcgaagcgc tggagttagc tcccacgacg ttgcaggccg
    41941 gagagactgc ttacttgcgc cggacatcgc gaacgaaagc aacccagccc cacgcgacaa
    42001 gcgcaccgaa cacgagaagg attcctgccg tgcccaagat gccccacatg cttcgcttga
    42061 gatagaacca tcgccagtag gcccagcctt gtgcaggctc tcgttcaaaa cccatcattt
    42121 gagctgagag gccatcggca atgaaggtgt atgcggccca tgtcgagatg agcacgagca
    42181 cggttgcgag catcaacacg aacgcacgga cagcgaactt gatgccgtgt ttcattgcgc
    42241 ccgcaaggct agcttcgtca agaagtcgat ggaacgatca agcttggcag cgagatcggc
    42301 gggcaaagcc tgccctggtc gtgatcgtat ttcgccaagg attgtcgcgg cttcgggctt
    42361 tgccgtctcg aacaccatgt cggtcaactc agtgaggatg ccttcatcgc gctcgttctt
    42421 gtaagaccga taaaactcga gccactgctc gggctgcggc gcaactcgcc tgaaggttcg
    42481 caaggcaagc aaccgaacaa cgccgttgtg tgaggccgcc atttgttgcc actcttgcag
    42541 ggtgctttgt cccaactgct cggtgcgagg aacgtcctcg aacgcatcgt taaacacctc
    42601 gaacaaaatt atatcacctt gagtgatgtt cgggtcgctt tggcggtcgg ctaaggcggc
    42661 gggcagcgag gcaccaccag ccgccgccgt cacaagcgca aggttgtgga gggcaagcag
    42721 tgagtcttgt tccgcagcgt cctgcgtctc gaatgctcca ctcaaaagct gcacaacaag
    42781 agggcgcttg tcctttggtg gctgaagcag ctcatgggtg ctcgccttgc tgccagcggc
    42841 acgaatgacg cggtcaaagc tggggagcca gtaatgctct ctgatctgcc gccaatgatc
    42901 acgagtcaat ccacctgcgg gctttccaat catgcttacg atctctgcca tcacgcgctg
    42961 tcgggcttgt ggtgcagtgc gtgaagcgtc gggtgtggat gattgaccgg gcaccgtctg
    43021 cgttgctgac ggagcttgcg aaggagtgtt gggagacgct ttggctggtg cagctttcgc
    43081 gtcggcatct gtggcgtgtc gatcaccctg tttgccgcaa ctcgcgagaa gcaaggcagc
    43141 ggctacaagt gtcacaaagc tggagttcgc attgttcatg gccgaggcgg tgcaggatca
    43201 agcgcaaggt agttgttcac gtagtagggg aacccgaagt tagttttccg tcccatgtag
    43261 gcacctcgca tcgttccagt tggatcaagg tgcatcaggg caagcgagcc agcgccactg
    43321 tccaagataa agagcttgat tgccccggca tcagcagacg gcgtgagcgc gggcactcca
    43381 gcagtgctgg cgtcagactt cacgatgggg ccatagccaa cgcccttgat ttgagtcgct
    43441 gtgaagatgc agcccttccc ggtttcttct cctttggcgt atccaatcat ctggtgtgcc
    43501 ttatccgcac gttcagcgct tgcatagttg gtggacagtc cgcccagcgc atcactgccc
    43561 ccagctcggc cagccgcgaa agtccaagcc ccagagttgc agccgagata ttttccaccc
    43621 ggatcacctg catatgggtt tgcaatgtcg tccgatgttc caggcactcc gtcaggcccg
    43681 gctagcgttc cggccggtat tggatcaggc cccgcgagtg ggcttccggc tgctggcttg
    43741 gtggtgttgt cgaagttgtc ggatgaagtc gtcgcggtga ctccgccacg aatgacccgc
    43801 ccgtggcact tgtctgcgat gttgccgagc atgttcggca aatcgaaagg gttcttttca
    43861 tcccaaccag cagaccagaa gcactggttg ccgttgacca ttacggcagg tgctcgcggg
    43921 acggcaggca gtgcaaaata agacatctcg atccccgctt gaccaccagc gagagcatcg
    43981 aacgctgtca tcttcgtcgc ctcctcgcgc atgacacaaa gacggaagat caccgatgga
    44041 ttcgggccga acggatagcc atgatcgcgg atgcacacgc gggcgttgtt ctccatgatg
    44101 tagtcacgcg cggcatggaa gaagtgcttc gctccgaagt cccgaactac agtcagcggc
    44161 agcttcaact ccgcccatgt ttcgttaatg gttttgccaa cgaacatccg caacgtgcct
    44221 tccgaatcga cgccagccca atcatccctg agccgcacgt aaatggtttc tggcaggctg
    44281 cctgtgctca agtcgaatgg cgatgaagcc gtgatgttga gtttatcgct agccgtgcat
    44341 tccttcgttt tgaagaactc cagtttgtcg atgacggaat gatcaaacca gacaacacct
    44401 gtgttgagac ctccaacgct taccttcaat gcctgcgcgt cgtcgtcgtc cttgtgtgcc
    44461 gccggtggtt tgggcatcgt gctgtaacca gacccgtatg agtatgacat cacgccatcg
    44521 tcatcggcat cccagacgcc attcgacatc ttgtcgttga tgaacagata ctcggtgccc
    44581 ttctccttgt cggcatcgct ggctcccgac tttgtggccg cttctctcaa ggaggaatcg
    44641 gcactcgtga tctgtccgtc gttgttcaaa tcagtcgcta agtcaaccgg caggaggcgc
    44701 agatggaagc tctggttctg ggtgtacgcc ttggcgattt cctcgctgct gaagtccacg
    44761 aggtgggtga agtcggactg ggtgccgttg tcgccgggtt cgattttgag aaggtgggtg
    44821 ccgagcacgg tgtgatcgta ttgtcctggc gcataggggg gcggtttttg gacttccttg
    44881 acgaggatga agctctgctc gtagccggcg ggcaggactt ccgtggagca cagcctgact
    44941 ttggcggcat gataatcaat ccaccgcatg tctttccagc tctcgctgcc gcttcccggg
    45001 tcatggttcg gattgttctg caggggagtc ggtgagaagt tggcgacgag ggcgggcttg
    45061 ggcctgccgt tgacggcggt ctcgtattgc ctatccgccg cgcgttccgg gctgcagggc
    45121 agggattcga gttcttccca ttcgtggtca gccggttcca agtgctcgct atggagcgcg
    45181 ctcgcgagcc aatcaggctc cgacgcgccg tcataccgcc agtagtattc tggccaagtg
    45241 ctgctgagtt ccggctgctc ggggtcttcc gcaggcttga tcaacttgca ctgcaggctg
    45301 gagaaagtgc tggggacata cgagccgatt tggtggccgt tctcatcaag ctcaggcacc
    45361 attttgtttc caaatttcca tgtgccgttt tgctcggctg tttccagata gacggtgaag
    45421 atttcagggt tctcggggct gccagtgccg tccggtccgc cgttggggcc gcctgttcca
    45481 ttggggccgc cggggtttcc cgttccgccc ggtttgtccg ggtcctgcac cacgggcacg
    45541 aggttttcca atctcagggt caggagacgc tgctggccag ggctgaaatc ctccaggtca
    45601 ggcgcgccaa ggtcgagctg gtcaatttgg gcggtgctgc tggatggagt gagcaaggtg
    45661 gatagatccg tggtgctcgt gacgggttcc ggttgagtgc cgtccgcatg atacaccgtc
    45721 tcctcaatga tgaccttgta gcgttggatc cagttgttca tgacctgggc ggtcatgtcc
    45781 tgggggcgca gccccggacg ccaggagatg agcactttgc cgcggtgata agcgccctgg
    45841 cagcggggtt cgaggaccgg ctggccggat gcgggcaggt gatgcatggg gtgaacctca
    45901 agttgtgact gtgcgatggc gacgatgccg cccgacgcgg gcattttcaa gggttgaaag
    45961 gggatgggat tgccattctg ggccagcgcg gccagtggat catgcacttc ttccgcaggc
    46021 ggagcggtcc agccggtttg cggcggggag gccgggccgc cggcagcgtt ctgctcgaag
    46081 aggtgcggcg gtgtcaactg ccccggccct gccgccggca gcaggggaat gctgggatcg
    46141 tggctggaga cgcgccaggt ctgggcgggg gtctcgtagc ggtggctgcc gtgggaggcg
    46201 agtttggcgc tgtagtcctc gccagggtat agggactccc acaaggattc gcctgtggct
    46261 ccgctgtgga cccaccacac ttgtttcaac tgcctgttga aatctagcgg ctggccgccg
    46321 ggaaaggcga actggcgcag gatttcaaac tcctggatgc gcgtgaccag cgctggatcg
    46381 ggcgtgtaag tgtgctcaaa ctcaaggtcc acagtccttt gggtgaaggg cggcacgggg
    46441 tcggtgtggc tgttgatcca gtcatcagca ggcatgtcgt atggaatggc ggcgtaatgc
    46501 gtggcggtct tctgtttctg cggacatgcc tggtccccct gcggcaggtg ccactgccag
    46561 gtgtccgtcc agcccagggt tcgggtctcg ccgacgtggg tccgcacctg gttgcagcgc
    46621 cagtcggcgt agtggatgac gaaggggctg tcctcgccaa agagaccgcc ataagtgttt
    46681 ttgtaggcca ggacttcggg cggcaggagg gcgtcggcat ccgggatggc gaaggcgaag
    46741 agcgccttgg cctggccgat gttggcgggg gcgaaatcgt catcgtcggt ggtgacggcg
    46801 gtccaggtga aaggcacggc cacgccgagt tcctggaggc gggcgtggaa gggcgcggcc
    46861 acggccttga gctggccgat gttcgccacc gcgtaatcgt cgcgcggggc ggtggcgggc
    46921 gccagccagg actgcaccag ggcggtgatc tgcggccccg cgccgcctgg cgtgcgcgcc
    46981 tccatctcca gaaaggcggc acgggcgagc tgcttgagct ggccctggtt gacgagggca
    47041 aagtcatccg gcgccagggc ggggtcgcgc gcctggcggg tgacccacca ttccggggcc
    47101 tgggagtgga gcatgccgcc ccaatggcaa acgagcagga gcagggcgac cgcgcgtttc
    47161 gtgtccgctt tcatgggcag gatggagtgg gagtgtgtgc tggttttgga ggagcaggcg
    47221 ggacccccgc tccccgcccg ccgctcaggg ctgcggcccc tcggtgaagg cccccatgct
    47281 caagtccccg cgcggctgga tgcgcaccgc gccgtggaag ctgacctcgc gcgcgaagga
    47341 cgcgccgccg ccgaaggtgg cgaagtccgc cgacagcgcg gtgatgacac tggcctcccc
    47401 ctccgaggca ggctgcttgc gctggatttc gagagtgttg ggctggatgc gcacctcggc
    47461 ggaccaggcg ggattttgca gccggctctc cgctcccggc gcgccggact cgtcatgcca
    47521 gaccgtgacg ccctcctcgg tgacgcggct gccccggctt tgacgctggg cgtcatcggc
    47581 ggccaggttc gacttcgaat acagcatctg gtagtaattc agcaggctga cgcggtcgtg
    47641 atccgcgctt tcgaaaggca gcggaaaaga cagccccgca gcgcccagct tcatcagcgg
    47701 acggtggaag ccgggcgcgt ccgggttgcg ccaaagccac tcggtgttgg agcggctgcc
    47761 ggtgaagcgc accaccggat gggtgtagtt ggtgatgtac tgcacctcct cgtaaggttc
    47821 cggcgtgatg gtgacttgaa agtagtcgtg gcccacgacg gcgtactgcc agtctgaaac
    47881 ccagtcattg gaaatcaaat acggctcgtc cagccagtag gggccgccgg ggtcgagcac
    47941 attgccgtcc tcgtcataag tcggcggctg gtaatactcc acggaatgca tgccccagac
    48001 ctcctgctgc gaacccatgt ccacccagtc ccagatttcc tggtattccg tggtcgtata
    48061 ggaggcggga gtgactgtga ccatgatctc ggtttcatac tgggtctgca gcacatcgag
    48121 ggtgaagccg gcgccgcccg tgtcatcaat gagggtgccg aagcgcgcgg actgctgaat
    48181 ggtcagatgc tcggcggaaa gctcggactg ggcctgcaaa acgaccggaa tgaggccgag
    48241 gaaagcggca atagaaagcc ggggagacaa cgcatgcggt ttcatgagag tggcttgatg
    48301 agtcttcatc cagcaagcac cgcgaggcgg catttgctga aattccccaa acgctgccac
    48361 aatccccccc cccccc
//
LOCUS       DAOMYJ010000009        47435 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Prosthecobacter sp. isolate AMR_MDS_5629
            NODE_290_length_47435_cov_24.144618, whole genome shotgun sequence.
ACCESSION   DAOMYJ010000009 DAOMYJ010000000
VERSION     DAOMYJ010000009.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867850
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prosthecobacter sp. (wastewater metagenome)
  ORGANISM  Prosthecobacter sp.
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Verrucomicrobiaceae; Prosthecobacter.
REFERENCE   1  (bases 1 to 47435)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 47435)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.30
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:34:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,742
            CDSs (total)                      :: 4,684
            Genes (coding)                    :: 4,676
            CDSs (with protein)               :: 4,676
            Genes (RNA)                       :: 58
            tRNAs                             :: 55
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..47435
                     /organism="Prosthecobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_290_length_47435_cov_24.144618"
                     /isolate="AMR_MDS_5629"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1965333"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..929
                     /locus_tag="PLP58_01725"
     CDS             <1..929
                     /locus_tag="PLP58_01725"
                     /inference="COORDINATES: protein motif:HMM:NF024117.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HRJ07272.1"
                     /translation="PAWRLRPRQAAARLETISPSRLASYLDCPFRHYLTSVLGMAGPD
                     PDQREMSALDFGNLAHQALQQLHAHEAMRRSTDEEALADFLVETAMRQAALRYGRRPA
                     PLVSLQLEGLKQRLRHAAKTEAAEREAGWRILHAEWSPEKPVLIEGAELRIQIDRVDR
                     HEGSGRIRVLDYKTSDKAADPLDSHAPKAGRKTTEQDEWRCFDAPDGQRRLWSGLQLP
                     LYAHALREHGLEPQEAGYFALPKSVQETRVLLWEDFGPAWIDGALECAAEAVRRMRAG
                     VFWPPSAKAREAGLDEIFLGDITGTVDPGFHA"
     gene            1015..3021
                     /locus_tag="PLP58_01730"
     CDS             1015..3021
                     /locus_tag="PLP58_01730"
                     /inference="COORDINATES: protein motif:HMM:NF025869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA-like domain-containing protein"
                     /protein_id="HRJ07273.1"
                     /translation="MPGAIHDIFISYRRADADKARTLKQGLEAHGLRCWMDSHIMPSQ
                     DFDEEIFKAISSARMLVVLLSAAVEQSPKQVRRELMIAEKYHIPIFPVRIDSAPLTRL
                     EFLLGTTQYLDVSQGDLACHLPDIADKARRLLEDLGEENEQAAPAPPQVAAVAPPQGN
                     GKVVSILYKRDAQPDERLLEFLEDRLGKLGYKIFHDRQLPLGIEWFKAIQEKVVSSHA
                     IIPLLSAVSIHSEMISMELRIAHEARQKQEGRPAILPVRVAYRGALPPEMSTYLDHLQ
                     YFAWESETQDEALLAALVQALEAPPKTAPVLEARHRQLPTGAVPMDSPFYIARPVDVE
                     MREAVTAGESIILVKGARQMGKTSLIARGLNLARQQKASVVLTDFQKLNNTQIESSEN
                     LFKALGSMMARQLKLDRYPEDTWHKSFGPNQNFENYLVDEVFPAVDGRLIWAMDEVDR
                     LFPARYSSEVFGLFRSWHNDRASLCGSDWENLTMIIGYATEASLFITDQTMSPFNVGR
                     QFRLEDFTRAQVEDLNERYDRPLNGPGEIDKLMELVGGQPYLTRRCLHEVAVRQTPFP
                     QLAASADRDDGLFSDHLRRFYILLCQPPEDGAEEPQPRKRGASEAPLMTAMTAFLRGE
                     GRLTDSQFYRLSSSGLLRGASPADAAPRCGIYERYLRRHLLGAS"
     gene            3018..10190
                     /locus_tag="PLP58_01735"
     CDS             3018..10190
                     /locus_tag="PLP58_01735"
                     /inference="COORDINATES: protein motif:HMM:NF025869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LpqB family beta-propeller domain-containing
                     protein"
                     /protein_id="HRJ07274.1"
                     /translation="MTRAALRTTNHAPSAMQPPSSSSEPFFKVGGTIGVNIASYVPRA
                     ADEEIFSALMKGDLCYVLTSRQMGKSSLMTRCSRRLIESGVTSVVLDLQRGGTELNAE
                     QWYNFLIDRTATALRLRPKVNAFREANPRLSPFQLWMEVLIQVVLVEAAAPVVIFVDE
                     IDLVRRFSFGTDEFFAGIRELYNRRTLEPDLCRLTFCLLGVASPADLIHDKRITPFNV
                     GRRIELNDLQPAEARLLSHGFAGRDPAVAERLVDRVMHWTGGQPYLTQKLASEVAARP
                     DAVSDSHVDACCGEIFFSPEGLKEFNIAFVRDRLLGEPHERERLLDLYRRVLHGKPVP
                     DEAADPAVAALKLSGAVREENGRLVIKNRVYARIFDAAFIKANYPVNWARRLRQAALA
                     SAAILLFACLPLAIFAWSQKMEAESGKLAAEQSAEDARKARDQAVESGAKAELARKEA
                     DRLREQSEKDRARFQDLLKSSTLKDLKRAEADARLVEAQAAWLFGKEHVWVARHFHPT
                     GRTSDRASWQFSSEQARVTEGRESLPARPRLPTVRPLTSRMVGAGVESMFPGPAGVGL
                     FAGTVLERERWDVDHKLFYFAPSGDAVEHGSHNARSIFTHPDMKAQVCAEAGSVLVTF
                     CPKEARLWDIAAGKSIGDPIAFEHEANEHAFPRRSAEWLALEMEDHVLVRHIATGKET
                     RLAFSPGAYVEKMAASADGSWLAAILVEDSKDDRVQLWRRAPEGVFQSGALIVEKEAA
                     GLEFSPGGGRLLVRLESGAVVHDVESGKQIGPKWQEIEEDDKISWSQDETMLQARREK
                     MLENGYLRKVRLNNKTQVEGDDLLVVPDTLLRHVCLSPDGKLAVTSGFDDALRLTYLD
                     GSSPVVEKKEAHEDNDINMAAFSPDGRLLASVADDNHVQLWSVPDLQPVASLAFSSST
                     AMGRSVVFDAKGSLVMAGDDEGYLSAWRVADRVKAWGLKVSEDDVTDLRVSPDGTRLA
                     LTSSNEVIVLDITAETKAAVEVSKWSAGTDVEYLQAAWSPDGTRLAAAGSEGAVHVWD
                     MTGKLEFGAEVQTREDTPTLAALFWKDGQELIFGGTQNVMGSLRLDDWDQNPQFPDAV
                     GDGVVASLALLPDGQTVLASGSGILEKKDLFWSEKDGAIAALPAGTRLQPSLPEAPVV
                     AEAAPDGLLDLSRRGEKRAEPDMSLRARLVDDSRNIVSVIPWPGQEGRLIAAANHGLL
                     GMDAGTIMDSSGLPSLDDLKLMAQKRIKEPAPGKAVNARGTPPRNNIPEASSAAAVVE
                     AENPQEKLLEKIDDATRLLSLASGEDEEVWVLLDTGLILALKRLGQAGGGWRIAWLAE
                     VPDDVENLEGVLEGLGGENVIESCRLAHSAATGHVLLLRDEQPDVEADDDVLNSSQAS
                     VWLLHPAQAPAQGFLRKLRASTLPLKGTLASTNSTGDYVVSATGRRFRGVNLSEWEEG
                     ADPALEPSLIEPGEIITALGLDPFVFHLAVGTRIGEILLYEHDAKAGEWLLRERFPAH
                     PGAVTSICWSSDGRWFASADDNGLVRFWNAAPGAFAAVEQSTDYPTLASLAAAWGVDY
                     DDVGDIRLKPRSDGIFQSCCMGAIPKPQPGIREALGRLDELRAVWEDPALEPLSAARK
                     RHDAAFQTTVESVKALGVTPMWLHPALKTATSWLLTAQAKDARAPLDFEHPAKDLIWP
                     LLALERLQNEGGTSVSLRLTHLAVIKQRMEAAARPAGEKSDPLSPDNVPEAWKADLEY
                     GQKLTLSLLGDLEAGELMQHEDVLHLFLTSEACLQRILDEKFLPKDDLLKLASTAEDE
                     RNNRLAFSLFQKAHERCWLSEAQHWVDYGLVTDRLKDFERSFMCFGMAAALSSGDESE
                     RAQQISNMSIVLNKWRGTDADFKSAAADLARIAWGMNSKQTNARTNYLKWREGSDLKK
                     KELLGLVEAAEKSGRDKAPYELQLASLQYGADDPNEAANARRMIAKAAASLPDDEDEL
                     RAAWEILEGKWDTSKERSEMLERFVVAVTAASNWMDSARFVKSRIAAAHREEWELADA
                     ITLFERVASDSVDLNAWCHAELADTYRRQGRLNTALEHGDKSIQLDATGDHWAWARRA
                     EVKAELGRWEEALADITEASRLRMQSEDANDYTQAHALLLAQKGDFDLALKLLEPKLA
                     KTREDEASNAGNGDADWLLNQADVLVWQAAASGRGIRVPESQELGREVLRWLDARKTA
                     KDAELQSKGLPPAKAKPPSLVRIRANLHEGNYQTAVQLAEERLQTWDHEVEAHLHHAA
                     ALSAAGRGDQALAAIARALSREYYGGHSNDKDRGVMPLSMNPWLLSIGAPIKDLIKRL
                     ELSDGEDEAALAEREIRIAEWIVKQPYAYTTALERAEVTRLAQACVNRALERFNYKPS
                     AGTPSILKSLLPRDAESGGLNSRTLR"
     gene            10262..10335
                     /locus_tag="PLP58_01740"
     tRNA            10262..10335
                     /locus_tag="PLP58_01740"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:10294..10296,aa:Gln,seq:ttg)
     gene            10354..10851
                     /locus_tag="PLP58_01745"
     CDS             10354..10851
                     /locus_tag="PLP58_01745"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007415561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0052717 - tRNA-specific adenosine-34
                     deaminase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deaminase"
                     /protein_id="HRJ07275.1"
                     /translation="MAEKREDLSEEDEGHLRRAIALSREKMLANLGGPFGAVIAKAGS
                     VVAEGWNQVISSKDPTAHAEVTAIRLASTRLGHFVLEGCTLYSSCEPCPMCLAAAYWA
                     RVDRVVYAATRADAAAGGFDDELLYRELPLPPDQRSLPMSQALHSEAARVFDEWRAKA
                     DKVMY"
     gene            10915..12519
                     /gene="nadB"
                     /locus_tag="PLP58_01750"
     CDS             10915..12519
                     /gene="nadB"
                     /locus_tag="PLP58_01750"
                     /EC_number="1.4.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009965016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008734 - L-aspartate oxidase activity
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-aspartate oxidase"
                     /protein_id="HRJ07276.1"
                     /translation="MTRFDFIIVGSGAAGLSAALHAARHGSVAVITKRGALDSNSNWA
                     QGGIACVTSDEDSIEKHVQDTLVAGAGLCREDAVRAIVTEGPARIAELVDWGVHFDQR
                     ESAEGHLEFDLTKEGGHSKRRVLHAADATGREISEKLLAAVRAQPAITVLENHFAIDL
                     VTTAKLGFVSEDRVLGLHVLRENDGSVVTLRSDRVILATGGCGRVYLYTTNPRVATGD
                     GVAMAWRAGASVANMEFIQFHPTCLYHPQKRSFLVTEAMRGEGARLIGADGREFMQDY
                     DPRGSLAPRDIVARAIDHEIKRTGGPCVYLDISHKPADFVISHFPTIHKACLEVGLDI
                     TRDPIPVVPAAHYQCGGVVTDVNGATRIRGLCAVGEVGCTGLHGANRLASNSLLECLV
                     LSRRAVVHMMKKLPVGKETAQDYEVPAWQSGDAVDNDELVVIYHNWDEIRRLMWDYVS
                     IVRTTKRLQRAAARLRNLKREVQEFYWNFRITSELLELRNLVETASLIVECAIRRHES
                     RGLHYTLDFPERDESRPPQDMVLRRY"
     gene            complement(12641..14044)
                     /locus_tag="PLP58_01755"
     CDS             complement(12641..14044)
                     /locus_tag="PLP58_01755"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase"
                     /protein_id="HRJ07277.1"
                     /translation="MLARLASLLLLAAATVSAADKPNIIFVFCDNLGNGDVACFNPLT
                     KHRTPHLDKMAAEGMKLTSFYSASGVCTPSRAAAMTGCYPRRVNLHVSNKGTFVLQPV
                     APKGLNSRETTVAEVLKTAGYATAIIGKWHLGDQPEFLPTRQGFDSYFGIPYSDDMTK
                     DKMPDVWPELPLMRDEKVIEAPVDRDYLTKRYTEEAIHYIEAHQDKPFFLYLPHAMPG
                     STQHAYSSPAFRGKSANGEWGDSVEELDWSMGEILAALKRLGLDEKTLVIWTSDNGAP
                     QRNPPQGSNLPYKGWGYNTSEGAMRMPCIARWPGKIPAGTECRELASMLDLLPTFAAL
                     GSATLPANPLDGHDIRPLLFGEAGAKSPADERGFFYYHMGQLQAVRSGPWKLYLPLEK
                     KLVSGKGRSAQAPLELYNVRDDIAEDREISAAEPEVVKRLLALAEAARIELGDEGREG
                     AGQRPAGWVENPTARSL"
     gene            complement(14068..15360)
                     /locus_tag="PLP58_01760"
     CDS             complement(14068..15360)
                     /locus_tag="PLP58_01760"
                     /inference="COORDINATES: protein motif:HMM:NF012312.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HRJ07278.1"
                     /translation="MSEAPSRVRHSVLAATTLAAFLMYLDRICMAEIVKSASFRGEFP
                     LTDNQLSWVLGAFFWAYALGQVPAGWLSDRFGIRPLMTVFIVLWSLFTALTGFATGMW
                     SLMLTRIGCGLAEAGAYPASSSLLRKWAVWSGRGTASGIVSLGGRLGGAAAPVLTVLI
                     ISSTGNWRWAGWSYGALGILFAWGFWRIYRESPEQHPRCNEAERALLKEGRPVESPVS
                     AGHSHRFPWAAMLRSGNLWFMCGAQFWTNVGWAFLATWMPRYLKEALKLSDEASGTLS
                     TVALLIGIGGILCGGFLTDAAVRLLGLKAGRLLPMAGTRFAAALMFVLALQTQNPWLL
                     VPALGMVAFFTDAGLPAVWATMQDIGGRHTAPLFGWANMWGNFGAALSPVFIALINAH
                     LDANGDWKEALWFCAAAFVISGFFALRINASEPVVKEA"
     gene            15468..15773
                     /locus_tag="PLP58_01765"
     CDS             15468..15773
                     /locus_tag="PLP58_01765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HRJ07279.1"
                     /translation="MPMRFRKIPESAQRNISGLRVREARLSSDPPMTQARLAAALTDL
                     GIVMDRVAVAKIETGQRCVFDFELKAFAAALNRPVEWLLGIGPRGTSGSAGVARGNK"
     gene            15865..16428
                     /locus_tag="PLP58_01770"
     CDS             15865..16428
                     /locus_tag="PLP58_01770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ07280.1"
                     /translation="MLLHEISREITEAHGAPAVTSESYASSVERAFGNACLYCARPLE
                     RNSTVVEHLDGMNRFRLGLHVPGNAALACKACNSQKRGDDQRAELILAGTGWESFLSH
                     DGTRCGLSCKSCAYWRDIIPDAEERRMFLAQRREAVARFRRHYLEILAAACGLRESLR
                     GQVTELYRACQDFASKEISALKRRVLS"
     gene            complement(16450..16878)
                     /locus_tag="PLP58_01775"
     CDS             complement(16450..16878)
                     /locus_tag="PLP58_01775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ07281.1"
                     /translation="MDPLNPNDPLWKLLGKARPVKARPNFTQNVLRAARQTPQEHGWL
                     AALRAWWSGGEKAPASLIWAAAAVALAALVVFMPEDKNASEIAGKTAPAMVEPLLLES
                     DFPLIEGYETEWENLEQMGALLAVQDTALLTDKEIHFLLY"
     gene            complement(17004..17762)
                     /locus_tag="PLP58_01780"
     CDS             complement(17004..17762)
                     /locus_tag="PLP58_01780"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: protein motif:HMM:NF006054.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004731 - purine-nucleoside phosphorylase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HRJ07282.1"
                     /translation="MSLARIQAARPETVIVLGSGLGGVAQTLGAEAEVPYAEVPGLTA
                     STVPGHAGRLVLTHVAGRAVLVAQGRRHLYEGLSAHEVAAGIRVMHGLGVRRVVLTNA
                     AGAIHTGFGVGELMLITDHLNLLGASPLTGGPNFHDMSGVYSAAWRAVFLEKAAALDI
                     TLHQGVYAATPGPQYETPAEIRMMRALGADAVGMSTVPEAIQARALGMEVAGISMLTN
                     WAAGIQPQTLDHAEVVTVGQSASIHLAELLKAAL"
     gene            complement(17759..18757)
                     /locus_tag="PLP58_01785"
     CDS             complement(17759..18757)
                     /locus_tag="PLP58_01785"
                     /EC_number="1.1.1.169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009959686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008677 - 2-dehydropantoate 2-reductase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HRJ07283.1"
                     /translation="MPDELEHSPRARMAAVEADEPAHSPLGRVAVVGAGAVGCYYGGR
                     LAQHGNDVRFLMRSDLAEVREHGLKILSPLGDLTLAVPAYGSTREMEECDLVLIAMKA
                     TSNEFLLELLPPLLHKDTALLTLQNGLGNEEFLAAHFGAKRVLGGLCFVCINRTAPGV
                     VRHIAQGRVSLGEHDRAPGERARRIAEEFRRCGIECVVEPSLAAARWKKLVWNIPFNG
                     LAIAEGGKDTAAILADAALEARVRALMTEIITTARALGHEVPFKLIPDMIERTRTMSR
                     YKPSSLIDFEEGRAVELEAIWGEPVRRARAAGVAMPLVEELHEQLRRRISTMNSAA"
     gene            complement(18828..19835)
                     /gene="ruvB"
                     /locus_tag="PLP58_01790"
     CDS             complement(18828..19835)
                     /gene="ruvB"
                     /locus_tag="PLP58_01790"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007413540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HRJ07284.1"
                     /translation="MNPALNEADSPFDLALRPGDFSEFHGQAKVKEQLLVMVEAAKMR
                     NEPLDHVLLCGPPGLGKTTLANLIANAVGSRLHTTSGPQIERAGDLAGILTNLQERDL
                     LFIDEIHRLHPSIEEYLYPAIEDFRLDIIIDQGPKARTIRLDLPPFTLVGATTRSGML
                     TAPMRSRFGIPNRLDYYSAEELRHILLRSARLLGVVMDEAGAHEIARRARGTPRIANH
                     LLRWVRDYAQVKAQNIVNEDVARQALTMRDIDESGLDEMDTRILEAVIHKFEGGPVGM
                     GSVAVAVGEDASTLEDVHEPYLVMQGYLKRTPRGRVAMPAAYRKLGLAPPLSGQAELF
                     G"
     gene            19981..21492
                     /gene="proS"
                     /locus_tag="PLP58_01795"
     CDS             19981..21492
                     /gene="proS"
                     /locus_tag="PLP58_01795"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012419096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006433 - prolyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HRJ07285.1"
                     /translation="MSQTAITPTREQDFPEWYQQVVRAADLAENSEVRGCMVIKPWGY
                     GLWENIQRQLDAKFKETGHVNAYFPLLIPLSYLEKEAEHAEGFATECAVVTHHRLEAQ
                     KQPDGTTKMIPTGELAEPFVIRPTSETIIGAAFARWVESYRDLPLLINQWANVMRWEM
                     RPRLFLRTAEFLWQEGHTAHETAEEAMVETRLMHKVYADFLRNHLAIPVIAGEKTENE
                     RFPGAVNTFTVEAMVQDRKAIQAGTSHYLGQNFAKAANIQFLGRDNTRQLAHTTSWGV
                     STRLIGTLIMAHGDDDGVILPPRVAPSQIVILPVTPKPDTRQEVIDACEALAQTLRAQ
                     TFHGEPLRVHVDKRDIPGGQKNWEWIKKGVPMRLEMGPRDITSRSVAVCRRDQGPKAK
                     EFIPKEEFLQTVTERLQEIQDALLARATEFRDQNFKKLDSLDEFKAFFDETAPGGFAL
                     MHWAGSNEEEDRIAREMKVTIRCIPMGDEFAGEGKCFLTGRPSIRRAVFARAY"
     gene            21629..21853
                     /locus_tag="PLP58_01800"
     CDS             21629..21853
                     /locus_tag="PLP58_01800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ07286.1"
                     /translation="MPSARQAAGLLQNEVIGDRFLDFFAFIAWPWGGWLEGIGQRAAS
                     FHLFGAAAQNLWFEKIKIFTNEAQTFKTAK"
     gene            complement(23105..23659)
                     /locus_tag="PLP58_01805"
     CDS             complement(23105..23659)
                     /locus_tag="PLP58_01805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ07287.1"
                     /translation="MIGLLVSSPSAVAAAVVPMSKLPTVVVVLPSVMLCVLLVTERKS
                     VIVKDPCTFTGPLRVVAVPRIVRFPPPVFVSVLMPAPLVLMLPVMTVLPALLMVRVRS
                     LPSLLFHALPVKVRVPPMLLVTVLLLLLDSCVHAPPKVTPYPLRLLKVAVVFAATRKV
                     PRLVRPLMLSPPTPGPVKTVEEAR"
     gene            23598..34598
                     /locus_tag="PLP58_01810"
     CDS             23598..34598
                     /locus_tag="PLP58_01810"
                     /inference="COORDINATES: protein motif:HMM:TIGR02601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter-associated beta strand
                     repeat-containing protein"
                     /protein_id="HRJ07288.1"
                     /translation="MLIGTTAAATALGLETSRPIILNGTTAANSIYANQAGLNPAKLN
                     GAITKPIAGNATFILGGTNTADNIINTGIPSLGTSGVTKVGPGTWVLAGENLYTGTTT
                     IVNGTLKLKANAASSTILAAANDITFGNSNVYAGGAFEFVGQDGVNNVQALDVLTTTS
                     GAGTVRLTPGVGGTASLSFASQATGAAGTVNFVGADFLNNKITITGANGLISRTNYWN
                     GSNFAYRESNVLRAPVYGTDAGFTTSASALTSAANMEITGSFATNTISISTLKIAGSR
                     TLTINPAQTLTLSAGGVLVTGGNALITGGTALALGSQALVVRTNLPGDSLEIQSVVTG
                     TGGLTKGGEGTLILAAANNQTGTVTINEGTIQLSGSGRLGAAAALTMRQGTILDLNGV
                     TPSTNTNAYNNNGSVTSSAPATFTVGGADGTGTSFGTVNGAISLTKVGTGAQSWLGSS
                     TYTGVTTIGSTGLVTVNTLANGGLPSGIGQSSNAASNLVFNGSTGGLVYQGNVVNGIL
                     NLDSASAATDRLFTLAGTGATLSSTVSNNNAIVWSSTGAIVHAVTGPQSLVFTGTSTG
                     DNTFNPQLTDSGSGANITSVTKTAAGQWNFGNPNNTYTGTTTISNGVLAFNDNGALPA
                     NSPLALGTTTTSGILQMSGTFERNIVATPAAGTGTISWIGTTGGGGFAAHASQLVVAI
                     GGTGSPTALTWGSGGFVPSGAALIFNSASALADVEFRNAIDFGAAVRTITVNDNGNTG
                     ADYATLTGDLSGAGGGLLKNGAGILRLYGVNSYTGTTEVQAGTLVVTSLGGSTGPATS
                     SVGASGVAMGDSNAIVLGNATTTGGLLQYVGPGETSDRKIRLRGTTAGNQIHADGSGP
                     LILTNVAHDTVETGSKTLSLRGTNTAGNMITSQLSDNVAGVLSIGVDGSATWILTNGS
                     NNYTGTTTVGSGALGIGHDTALGTGNLIVSNGNLFAFGGDRTIANTLQMNSGTSHGFL
                     GDHSLTFTNAVNLAASTSSSNFTNVSIVPGEAVIFDGGVFADALTATRNWSIEGQGEL
                     VINGSFTTSTAFGVNIIKTGNGTLTLGTDGSGSNWNQAGNAVDVDRGVLKFAADNAIP
                     TAASTNGGLTLSPEVLTTDTATVDLNGTTQTVNAITMNTDGVAIIDNTSAMAATLRFG
                     ANDTVASIGGGIGTYTVTDSGAGALSLVKLGNTSATIPTGVTLTYQGGTGVEGGTFTI
                     ASPVSGTSSLSVINSGSILALTGGITSPSAITSVVVENGGTLSLLDGAGNKLDSLTNL
                     QLGSSGGTMSTLNLNVGDSLVAGDELNTDTLALLTGGTLSLFSGNQITFNLTDIGLSP
                     NQQYVLLDATAIGGGLFGGPLNIGDYILGGTPGGFSFIDLTTNSTTNQIILTTGNLIL
                     GTSYWRGLTDSTWNAAANNWSTDKAGTTPALSIPGAGTDVVFAWDNPGSGPLVTTLEQ
                     NFKVNSLTFEAGGTTTPSSVTINPGTVATNRIEIAPQMAGDGIEISAGGPAAVTISGP
                     VRLGGTATSQTWTVADAGSVLSLGSLAGERDVTKAGSGKVVLTTAADPTFNGGVTTDF
                     TINAGTLEILDVSALGNPINSNLANVTVNNTGVFFYNGAASTATNLAMPITLNGGTLS
                     AGNAPAPTTTITQNYSGPVNVSGNSFINMANSNGPATNTAQNITLSGVVSGSGGLTVS
                     SNTTTLTGGNPESGTLLISNAASTWSGPLTLTSGTVDIGVAASPTVVPSSVTFDAFGR
                     LIVRGVNGQTYDLAGAMNFTAGTIGEYLLDNTSAVLAADFVVNQDGQVNLGSGGTGAS
                     ARFTVNDAATLLNINGNVVLGGNSSISVDGGDADSFVTISGVISDGGSGYSLAINDDA
                     GGWAITNDIIRLAGLNTFTGNISLDSGILEFNTVTGISGGASSLGNGTAILTTNAANL
                     RFIGSSAQSTNRPINTAGGALTLSANGATAADTITYNGLITVGSTADGSQIVLTGAAG
                     REGIITGGIMQTGDAADFTVNGGLWTLQTGTSRIGDDATVTGAGTVLDVDSGVLQVRD
                     DFTVTANGVLNLNAAGVLTFNIATLSADASLRATNGGVINLGASNAVVTTEFDGLRIG
                     TDAAGIGTLNMNAFNQSVTEFILGNRQSDRSGIVNGTGVLTVTGNLDVYGGSINANLA
                     STGTNTFEKISLNTVTLSGDNTGLASTGASIVYEGTLILDYTSSNTTKLRAASALDIR
                     GGHLQLNGNASAATSQSVGSITLASGIGNSLITLNPGTGQDLVLNLGAITRAVNARAG
                     TLRVILPSGTQSATNGVTTTTGLTNGLVGTAGYLTVEDGTGTWFATKSGNNIVALAST
                     AKNDISTWAAGDHITDETTGFTGTFASVSINSLRFDAAGGSDINLGGTGVLGIATGGL
                     LVTDNVGGTPSLIGGTIFSGALASNVPELILTHDGSTNFILGSDLRTNTAFVKSGAGT
                     AVLSGNNVSTGDVSVQNGILQLSGGNAIGDTARVTLANHRNTVLELLASETIGRLEGG
                     QRQTNGDWGVVDVGSHTLTVNQTANSTYSGRFAGTGTVIKSGASIMTFAGHDAANGFT
                     GTFSVAQGLVVLSGAGSQLNGASDIVLSGSGSSLRFDNDQTTAVGSRITDIATITLSS
                     TAGTTADSLGFYMRRTAGTTTGTETVGQLVLNTGHNTIAADGTATDRIARINFVNAVP
                     LVRNNLSTLLLVARNFAATAGQRGRISFSVAPSGAIGGGGAAGTSTLNIYPYMVGEDA
                     SGAPSGAANFGNTFVYYETGTTDMRPLNLTTEYVVGEASFNSLGAGVLANNVRFTASP
                     GAALDSDTTGVNALVLDNATGITIGGAAQPLQITSGAILSAGAGANVINGFTSLTTGA
                     GNPYYIYVTNPAGTLTLAATTLGSSEALVKSGAGALILGAANSVTSVNINQGVLEIGD
                     LDHIGGDAGDLRFAGGTLRLGAGFTDDISTRAISFLIGGGTLDTNGIDLTLAGSLGSG
                     AGGFTKAGAGNLTLNGTATYTGNSLLSLGTVTIGANNALGNGGNLTLAAGATLSFSGS
                     QSLSHGLVTTSGASPAIIGSGTITASRGFLFNNTGDIAISAVLAGAGGVFKAQTNMLT
                     LAGLNTYVGTTEVQAGTLSFDSIGNVGGGASALGNPATAQAGIIRMGLTTAATTLTYT
                     GSGHSSNRLIGMQGTTGGVTLNADGSGAISYGGARFENAGNKNLTLGGAADSALINSI
                     GGLSELGGVMSLIKTGGSTWQLSAANNHSGATTVSEGILSITNSGALGTTAAGTSVGT
                     GGVLQVSNNVSVTGEALTLTSGAGGSATLSNLSDSNTWTGNLTVDTGADASNRAVLNS
                     DAGNLLISGSINLSSGTQDFVLRGDGNGEISGQITGSQRLFKSSVGAGTWTLSGDNSS
                     TFTGRATVGNGALQVSSEANLGATPGSFEANQLTLGGGSANGALKTTATMSLSANRGV
                     TLGTMGGTFDTAAATTLTVNSVIASAAGALVKIGTGTLSLAGNNTYVGGTTVSVGTLL
                     AVNTLGSATGAGPVTVDSGATLGGNGFIAPGVDGNITVNGTLQVGAAGDASAQQLTIT
                     TTGVGLVTVNNIVVFDLFSGQGSGVLNDATHNDQLVVNGSNGFAIGAGAQLHVSTSLP
                     IDGTWVAGSSWRLFDWSGLTGGVTGEFSNLSDPAPFNYMNLPDLSSIGLAWEYSAGTL
                     TVVVPEPGRMLLLFLGLMGLFFRRRR"
     gene            complement(34886..35332)
                     /locus_tag="PLP58_01815"
     CDS             complement(34886..35332)
                     /locus_tag="PLP58_01815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ07289.1"
                     /translation="MSEPASTQFQPRESALRSYLQLAAYGIPCLFFVVFADLVLIPRM
                     LKIWSEVGGKEPEANWIFDLCSIFVVYFQFFAGAAAALFVILEKTWPAWQRLRSPVLR
                     ITAWLLNFAVMLGMTWMAVAALLVAPKAVAMHRQKLEPNGGSKAPP"
     gene            complement(35344..36627)
                     /locus_tag="PLP58_01820"
     CDS             complement(35344..36627)
                     /locus_tag="PLP58_01820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="acetylxylan esterase"
                     /protein_id="HRJ07290.1"
                     /translation="MRPLPILLTAMILTGEDFAEAQQSKANYDESLAGSYTLPGLLTR
                     ADGSVVKTAEDWQKHRRPEILALYQQHVYGRVPEGLGAPAFSVKSVKTDALGGKATRK
                     LIQITLPSCPAWEGMELMLYTPNGLNRPAPCFAGLSFGGNHAVSTETDVPISTRWMRP
                     SKEKHIVDNRATEASRGNESSRWPLEMILSRGFAVATAYYGDIEPDHAEGWKDGLRGL
                     TSPAGAATRWKEGEWGAISAWAWGLSRILDYLETDRDVDAKHCAVIGHSRLGKTSLWA
                     GAQDERFAFVISNNSGEGGAALMRRNFGETTAVITRAFTHWFTTTYKNYAGNEAACPV
                     DQHFLVALAAPRPIHIGSAVEDQWADPKGEFLSGYHASPVYELFGKLGTGVSEQPAID
                     TPVGHSIGYHVRTGGHDVKDYDWEQYMTFAEKHWR"
     gene            complement(36680..38410)
                     /locus_tag="PLP58_01825"
     CDS             complement(36680..38410)
                     /locus_tag="PLP58_01825"
                     /inference="COORDINATES: protein motif:HMM:NF014036.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dynamin family protein"
                     /protein_id="HRJ07291.1"
                     /translation="MIGDEYFQLRTRLSAELQRLAGLIRDLGGDAESAAIAENLIASL
                     RDPFVFVVVGEVNVGKSTFLNALFGQDFSRTGVVPTTDKILFFKHGDRQQIVPVTPTL
                     DEVHVPSDFLRDFHIVDTPGTNSIENEHQEITERFVPVADLVIFVFSAMNPWGASAWQ
                     FLDKVHKHWMRHVIFILQQCDLRSHEEIQVITDYMAQLTSLRYGQHFPIFAVSAKKAY
                     LARSSGLDRERLLEESGFLPLESHISSLVGHNAARLHKMAGTTRLARQLLENLRQSVE
                     STAADSRRALALIQEFITERELQVDRTLKKILPALDATGRDYHECCLLVAGMAEHVLS
                     TKRAFEKNGEEDEDFGGHRKDSLDHRLFQDLQHRTGDRWRQVGIILEEDFLQYARFLH
                     VQGRGTLFAHDAPLPVDADADLRRLFSVHMDSTMRRFVLGLKLDEALEPGLRKARRRA
                     RWVPWLLLPVLAAIGAAWWFDGPLGAAIAGGGGLLLAGFAFLITQTALNHTRGLLMDR
                     LEESSELLRDMLHRQIHSDVENLFSRFLPLLAPAQEEAARREQEMSSQTGRIADLARS
                     LGEFEQSLRA"
     gene            complement(38527..39414)
                     /locus_tag="PLP58_01830"
     CDS             complement(38527..39414)
                     /locus_tag="PLP58_01830"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HRJ07292.1"
                     /translation="MTPQRMYLSIWFGILLGGSGCTSKDENLAGLDEVRRAVPLEEMA
                     PSAQPNPLAKRVQQPSTLPTAPPAGVKISYSSVNTQEKILAMTFDDGPHPSLTPKLLD
                     ICKERNVKCTFFVVGRNAKAYPAIIRRIIEEGHEIANHTYTHPSLTSRSDAQIRSELK
                     LSEDALMAAANYRPHLIRPPYGAINTRIKQLMFSEFGYSTIMWSVDPQDWRRPGVSVV
                     TNRLVSGARPGAIMLAHDIHPPTIEAMPAVFDQLIAQGYQFVTVSQLMNLEKANMPVG
                     MIISPALPVSEPETGRLTN"
     gene            39510..40811
                     /locus_tag="PLP58_01835"
     CDS             39510..40811
                     /locus_tag="PLP58_01835"
                     /inference="COORDINATES: protein motif:HMM:NF012401.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite oxidase"
                     /protein_id="HRJ07293.1"
                     /translation="MKYAILRRYFWIMLQARTPSPPIPPSVSRRGFFERLSLGSMTAA
                     LGAEIVFAGKMPAGLIPVALAQTAPPDIEGKDGLSILNDRPLVAETPAHLLNDAVTPE
                     KHLFVRNNGLAPAPELLDPDKWTLEIAGESCERPAVFTLKELKQKFAHHTLRMVLECA
                     GNGRSEFNPPATGNQWTTGGVGCPEWTGIRLGEVLRHCGIKKDAVYLGYHGADLHLSG
                     NPEKSAISRGVPIQKALEDESLLVFAMNGADIPWQNGHPLRLMFGGWAASTCGKWVKK
                     IVIRDREHDGEKMTGNSYRMPRYPVAPGTKVPPEDMVIIEAMPVKSLVTFPKSGATHP
                     AGEKLAVRGHAWTGEGSVASVEVSLDFGSTWGRAALNAPANRYAWQQWRAEVGFPKKG
                     YYEIWARATDDQGRSQPMILPGWNPEGYLNNSCHRVAVQAV"
     gene            40896..41237
                     /locus_tag="PLP58_01840"
     CDS             40896..41237
                     /locus_tag="PLP58_01840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ07294.1"
                     /translation="MKIAPGWEITRNHCVICHSPQTFLRQRATEANWTSVLEWMQTNG
                     GLWKLEPAVQKDIIAYLAANYGPGDSQNYRRAPIPATLMPPNPYATEARLEAEEKKKQ
                     GLIPAVKPPGP"
     gene            41234..41896
                     /locus_tag="PLP58_01845"
     CDS             41234..41896
                     /locus_tag="PLP58_01845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009965902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HRJ07295.1"
                     /translation="MTPAALITGGKGALAVALRAELEAQGWQVHAPGREVMDVTDPGS
                     VSDFTGALERLDLLVHNAGLILDAPLAKMSAASFDTVLQAHLRGAFLCARAALRLMVR
                     KRAGHLVFIGSRSALTGPAGQANYAAAKAGLIGLAQSLAAEYGPRGIRANVVLPGFLE
                     TPMTASLLAQPEKRARVMAEHSLGHLNTCAEAARFIVFLHTLPHTSGQVFHLDSRIHR
                     WA"
     gene            42115..44061
                     /gene="dnaK"
                     /locus_tag="PLP58_01850"
     CDS             42115..44061
                     /gene="dnaK"
                     /locus_tag="PLP58_01850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012420444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HRJ07296.1"
                     /translation="MSKILGIDLGTTNSCMAVLEGGKAVVLENSEGARTTPSVVAFTK
                     SGERVVGQAAKRQAVTNPRNTVFSVKRLMGRKFTELTEADKRMPYKIVAAANGDAHVQ
                     VEVGGETKTYSPQEVSAMILSKLKADAEAKLGETITEAVITVPAYFNDSQRNATKAAG
                     EIAGLNVRRIINEPTAASLAYGLESKSDEKIAVYDLGGGTFDISVLEIGDGVFEVLAT
                     DGDTHLGGDDWDNTLITWIVNEFKADTGIDLSGQPDAIQRIKEEAEKAKIALSSSQSY
                     DLNLPFITADQTGPKHIMKTLSRAKMEQLCDSLFERTMKPVRDCLAAAKLDASKIDEL
                     VLVGGMTRMPKVVETAKKLAGKDPHQGVNPDEVVAIGAAIQGGVLKGDVKDVLLLDVT
                     PLTLAIETAGGVATPMIPRNTTIPKKHSQIFSTYADQQPGVEIVVLQGERPMSRDNKT
                     LGTFKLDGIPPMPRGVPQIEVTFDIDANGILHVSAKEKTTGKEQKISIQGSSGLSQDE
                     IEKAKRDAEEHAEEDRKRKESVEVKNKADSLTYEIEKTHKEWEGKVPAEQLAPITGKL
                     ASLKSAIEANDTEAMKTQTEEIEKLFAAAYQAAAAAGAGAPGAGGMPDMSGMGGAEAP
                     APETKSQDKGKVVDADFEVVDKDK"
     gene            44106..44762
                     /locus_tag="PLP58_01855"
     CDS             44106..44762
                     /locus_tag="PLP58_01855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ07297.1"
                     /translation="MNRLIEFLKSRSPDERMGLKTIVEADSLRSVDIADALRWKASTL
                     LEYHFQQPLTYYQVVQRVARRLEIRADDGDTVEELEKQICSKVFETMWNKMSDAEKQE
                     FEKKIKEAAKENDDNINWVSGGSVAAAIIAGKMSGFAIYIAASTALSVLSTGLGLSLP
                     FVAYTTVSRVISTVLGPVGWIGAGLLTAYQISGPAWQRVTSAVAYISMLRNTPPPKRS
                     "
     gene            44833..45726
                     /locus_tag="PLP58_01860"
     CDS             44833..45726
                     /locus_tag="PLP58_01860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ07298.1"
                     /translation="MNGELRGLRQPCCRFGVGAALLPGVDSRLRAELSDSATHSRSKP
                     AGRRPGVFGELSAVVTLTLRRRTARLMRLARTSSQPGDLAKQTHPRSSAKAGRANATP
                     GLLLALLASFVLVSCGKQEAAKHAVEAPKPAATSPAPVISDSRLDAEISQALTPTAAE
                     RDEGLADLAEDILDKYPNKNVTELLNVPEVNESLKVALTKLSENKALQNQINGTVALA
                     AQMQGLNGEPGSTRLDLDTSNYDHARKSRLLQVVLSEDPRRIAGFIAQEIGEAVPELT
                     FEGVDRASNGVAIRENAPPQK"
     gene            45861..46157
                     /locus_tag="PLP58_01865"
     CDS             45861..46157
                     /locus_tag="PLP58_01865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020494119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HRJ07299.1"
                     /translation="MATSITPLGRRVLVKRSTSEEKTAGGIFLPDTAKEKPQEAEVLA
                     LGTGNDDEGKDVSTLFTVKKGDKVLISKYGGTEVKVDGEDLLIINETDILGIIG"
     gene            46318..>47435
                     /gene="groL"
                     /locus_tag="PLP58_01870"
     CDS             46318..>47435
                     /gene="groL"
                     /locus_tag="PLP58_01870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004585059.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HRJ07300.1"
                     /translation="MAKQLHFDETARQALLRGVTKLAKAVKATLGPAGRNVILEKKFG
                     SPTITKDGVTVAKEIELPDPYENMGAQLIKEVSSKTSDIAGDGTTTATVLAEAIYAEG
                     LRNVTAGANPISLQRGIMKATEAIVAKLKEISTPVKDSKEVAQVATVSANWDAGIGNI
                     IAEAMDKVGKEGTITVEEAKSIETTLEVVEGMQFDKGYLSPYFVTNPETMEANLESAY
                     ILIHEKKISSLKDMLPLLEKVARSGKPLLIIAEDVEGEALATLVVNKLRGTLNIVAVK
                     APGFGDRRKAMLEDIAILTGGRCITEDLGIKLENIELADLGQAKRITIGKENTVIVEG
                     EGSSDAIQGRVSQIKRQIEETTSDYDREKLQERLAKLAG"
BASE COUNT         9521 a        14631 c        14544 g         8739 t
ORIGIN      
        1 caccggcctg gcggctgcgc ccgcgccagg ccgcggccag gctggagacc atctcgccat
       61 cgcgtctggc atcctatctg gactgcccct tccgccatta cctcaccagc gttctgggca
      121 tggcggggcc ggaccctgac cagagggaga tgagcgcgct ggatttcggc aatctggcgc
      181 accaagcctt gcagcaactt catgcgcatg aggccatgcg gcgcagcacg gacgaggagg
      241 cgctggctga tttcctcgtg gagaccgcca tgcgccaggc agcgctgcga tacggacgca
      301 ggcccgcccc tctcgtctcc ctgcaactcg agggactcaa gcaaaggctg cgccatgccg
      361 cgaagaccga ggctgcggag cgcgaggcag gctggcgcat cctgcacgcg gagtggagtc
      421 cggaaaaacc cgtgctcatc gagggcgctg aactgcgcat ccagatagac cgcgtggacc
      481 gccacgaagg atcgggccgc atccgcgtgc tcgactacaa gacttccgac aaagccgcgg
      541 acccgcttga cagccatgcc cccaaggcgg gcagaaaaac aacggagcag gacgaatgga
      601 ggtgcttcga cgcccccgac ggccagcgcc ggttgtggag cggtctgcaa ctgccccttt
      661 acgcgcacgc tttgcgcgag catgggcttg agccgcagga ggcgggttat tttgccctgc
      721 ccaaaagcgt tcaggaaacc cgcgtgctgc tctgggaaga tttcggcccg gcctggattg
      781 acggcgccct ggaatgcgcc gccgaagccg tccgccggat gcgcgccggc gtcttctggc
      841 cgccctccgc caaagcccgc gaggctggcc tcgacgaaat tttcctcggc gacatcaccg
      901 gcacggttga tcccggcttt catgcgtgag agtcggggaa agccttgcgc aaagtttcgt
      961 gttgtcgtcg caagaagctg gcatttcatt ttttgttggc gtatttgaaa acccatgccc
     1021 ggagcaattc acgatatttt catcagctac cgtcgtgccg acgcggacaa ggcgcggacg
     1081 ctgaaacagg gactcgaagc tcacggcctg cgttgctgga tggacagcca tatcatgccc
     1141 agccaggatt ttgacgagga gatattcaag gccatttcct cggcaagaat gctggtcgtt
     1201 ctgctctccg ccgcagtgga gcaaagcccc aagcaggtgc gacgggagct catgatcgcg
     1261 gaaaaatatc acatccccat ctttcccgtc cgcattgaca gtgctccgct cacccggttg
     1321 gagtttcttc tgggcaccac acagtacctg gacgtgagcc agggtgatct ggcctgccat
     1381 cttcccgaca tcgcggacaa ggcgcggcgt ctgctagagg atctgggtga ggaaaatgag
     1441 caagccgctc ccgcgccgcc ccaggttgcg gctgttgcgc caccccaggg caatggcaaa
     1501 gtggtctcca tcctttataa aagggacgcg cagccggatg agcgcctgct ggaatttctc
     1561 gaagaccgtc ttggcaagct cggatacaag attttccacg accgccagtt gccactgggc
     1621 atcgagtggt tcaaagccat ccaggaaaag gtggtcagct cccatgccat catcccgctg
     1681 ctgtccgctg tctcgatcca tagtgaaatg atctcgatgg agttgcgcat cgcccatgaa
     1741 gcgcggcaga agcaggaggg gcgtcctgcc atcctgcccg tgcgcgtggc ctaccgcggc
     1801 gcgctgccgc ctgagatgag cacttatctg gatcatctcc agtatttcgc ctgggagagc
     1861 gagacgcagg atgaggccct gctggcggcg ctggtgcagg ctctggaagc tccgccgaaa
     1921 accgcgccgg tgctggaggc caggcaccgc caattgccca ccggcgccgt gcccatggac
     1981 agtccttttt acatcgccag gccggtggat gtggaaatga gggaggcggt cacggcggga
     2041 gagagcatca ttctggtgaa gggcgcgcgg cagatgggca agacctcact catcgcgcgc
     2101 gggctgaacc tggcgcgcca gcaaaaagct tcggtggtgc tcacggactt tcaaaagctc
     2161 aacaacacgc agatcgagtc ctccgagaac cttttcaagg ctctcggcag catgatggcg
     2221 cgccagctca agctggaccg ctaccccgag gatacctggc acaagtcctt cggcccgaac
     2281 cagaactttg agaactacct tgtggacgag gtcttccccg ccgtggacgg ccgcctcatc
     2341 tgggccatgg acgaggtgga ccgtcttttt cccgcccgtt acagcagcga ggttttcggc
     2401 ctgttccgct cctggcacaa cgatcgcgcc agcctctgcg gcagcgactg ggagaacctg
     2461 acgatgatca tcggctacgc caccgaggcc tccctattca tcacggatca aaccatgtcc
     2521 cccttcaatg tcggacgcca gttccggctc gaggacttca cccgcgcgca ggtggaggat
     2581 cttaacgaac gctacgaccg ccctctcaac ggccccggcg agattgacaa gctcatggag
     2641 ctggtgggcg gacagcccta cctcacccgc cgctgcctgc acgaggtggc cgtgcgccag
     2701 actcccttcc cccagctcgc cgcctccgcc gaccgggatg acggtctctt cagcgaccac
     2761 ctgcgccgtt tttacattct gctctgccag ccgccggagg atggcgcgga ggagccgcag
     2821 ccgcgcaagc gcggagcttc cgaagctccg ctcatgaccg caatgaccgc ctttctgcgc
     2881 ggcgaaggca ggctgactga cagccagttt taccgcctca gcagcagcgg cctgctgcgc
     2941 ggagcctcgc ctgcggatgc cgctccgcgc tgcggcatct acgagcgcta cctgcgccgc
     3001 cacttgctgg gcgcctcatg acccgcgcgg ctctccgcac cacgaatcac gcaccctccg
     3061 ccatgcagcc tccgtcttct tcgagcgagc cgttcttcaa agtcggcggc accatcgggg
     3121 tgaacatcgc ctcctatgtc ccgcgcgccg ctgacgagga gattttttcc gccttgatga
     3181 agggggacct ctgttatgtg ctcacctccc ggcagatggg caagtcctcc ctcatgaccc
     3241 gctgctccag gaggctcatc gagtcgggtg tcacctccgt ggtccttgat ctccagcgcg
     3301 gcggcaccga gctcaacgcc gaacagtggt acaatttcct catagaccgc accgccactg
     3361 cattgcgcct gcgccccaag gtgaacgcgt ttcgcgaggc aaacccgagg ctaagtcctt
     3421 tccagctctg gatggaggtg ctcatccagg tggtcctcgt cgaggccgcc gcgcccgtcg
     3481 tcattttcgt ggatgagata gacctcgtcc gcaggttcag cttcggcacc gacgagtttt
     3541 tcgcgggcat acgcgagctt tacaaccgcc gcaccctgga gcctgacctg tgccgtctga
     3601 cgttctgcct gctcggcgtc gccagcccgg cggacctgat tcatgacaag cgcatcaccc
     3661 ccttcaatgt cggacgccgc atcgaactca acgatctcca gccggcagag gcgcgattgc
     3721 tctcccacgg ctttgccgga agggatcccg ctgtggcgga gcggctggtg gacagggtca
     3781 tgcactggac tggcggccag ccctacctca ctcaaaaact cgcttccgag gtcgccgcta
     3841 ggccggatgc cgtcagtgac agccacgtgg acgcctgctg cggggagatt ttcttcagcc
     3901 ccgagggttt gaaggagttc aacatcgcct ttgtccgcga ccgccttctg ggcgagccgc
     3961 acgaacggga aaggctgctc gatctctacc gccgtgtgct gcacggcaag cccgtcccgg
     4021 atgaggcggc ggaccccgcc gtggccgcgc tcaagctctc cggagccgtg cgcgaggaga
     4081 acggacgcct ggtcatcaaa aaccgcgttt acgcgcgcat ctttgacgcc gcctttatca
     4141 aggccaacta ccccgtgaac tgggcgcgcc gtttgcgcca ggccgcgctc gcctccgcag
     4201 ccatcctgct ttttgcctgc ctgcctttgg ccatctttgc ctggtctcag aagatggagg
     4261 cggagagtgg aaagctggcc gccgagcagt ccgccgagga cgcaaggaag gctagagacc
     4321 aggcggtgga atcaggagca aaagctgaac ttgcgcggaa ggaggctgac cgtttgcgcg
     4381 agcagtctga gaaagaccgg gcgcgctttc aggacttgct taaaagctcc acgctcaagg
     4441 acctcaagcg cgctgaggct gacgcaaggc tggtcgaagc ccaggcggcc tggcttttcg
     4501 gcaaggagca tgtctgggtg gcgcggcatt ttcatccaac cgggcggaca agtgaccggg
     4561 cgtcatggca atttagcagc gagcaggcca gggtgactga gggtcgggag agcctgcccg
     4621 caagaccccg ccttcccaca gtaaggccgc tgacctcgcg catggtggga gccggggttg
     4681 aaagcatgtt tcccggccct gcgggtgtag gtctgtttgc ggggacggtt ttggagcgtg
     4741 agcggtggga tgttgaccac aagctgtttt actttgcgcc ctccggggac gctgtggagc
     4801 atggttcgca caacgcgcgc agcattttca cccatcctga catgaaagcc caggtttgcg
     4861 cggaggctgg gagtgtgctg gtaactttct gccccaagga agcccgcttg tgggacatcg
     4921 ccgcaggcaa gtccattggc gatcccatag cattcgagca tgaggccaat gagcacgcct
     4981 ttccccgccg aagcgccgaa tggctggcgc tcgagatgga ggatcatgtg cttgtccggc
     5041 acatcgccac gggaaaagag accaggctgg cgttttcacc cggcgcttat gtggaaaaaa
     5101 tggccgccag cgcggacggg tcatggctgg ccgccatcct cgtcgaggat tcgaaagatg
     5161 acagggtgca gctttggagg cgcgcgcctg aaggagtgtt tcaaagcggc gcgctcattg
     5221 tggagaagga ggctgccgga ctggagttct cacccggtgg aggccggctg ctggtgaggc
     5281 ttgagagcgg cgcggtggtt cacgatgtgg agagcggcaa gcaaatcgga cccaaatggc
     5341 aggaaatcga ggaagacgac aaaatctcct ggagccagga cgagacgatg ctgcaagcgc
     5401 gaagagaaaa aatgctggag aacggatatc tgcggaaagt ccgcttgaac aacaaaactc
     5461 aggttgaggg ggatgatctt ctcgtcgtgc cggacaccct gctgcgccat gtctgcctca
     5521 gcccggatgg caagctggcg gtcacttcag gatttgatga tgctttgcga ctgacatact
     5581 tggatggctc cagccccgtc gtggagaaaa aggaggcgca tgaggacaat gacatcaaca
     5641 tggcggcctt ttcccctgat ggccggcttc tggcaagcgt cgcggatgac aatcatgtgc
     5701 agctctggtc ggtccccgat ttgcagcccg tggcgtcact cgccttctca agcagcaccg
     5761 cgatggggcg gtccgtggtt tttgacgcca agggcagcct ggtgatggca ggggatgatg
     5821 aaggatacct ttccgcctgg cgcgtggcgg acagggtgaa agcctgggga ctgaaagttt
     5881 cggaggatga tgtgacggac ctgagagtgt ctcccgatgg aacccgcctc gcgctgactt
     5941 caagcaacga ggtcatcgtc ctggacatca cggcggagac aaaagcagcg gtcgaggtgt
     6001 caaaatggag tgccgggaca gatgtcgaat atttgcaggc cgcatggtcc ccggatggaa
     6061 cccgtctcgc ggctgccggg tcggagggag cggtgcatgt ctgggatatg acggggaaat
     6121 tggagttcgg cgccgaggtt caaacccgcg aggacacgcc gactctggcg gctttgttct
     6181 ggaaggatgg gcaggagttg atttttggag gcacgcagaa tgtcatgggc agcctcaggc
     6241 ttgatgactg ggatcaaaac ccccaatttc ctgatgcggt gggtgatggc gtggttgcct
     6301 cactggccct gctgccggat gggcagactg tgctggcctc aggatcgggc atcctggaaa
     6361 aaaaggattt gttttggtcg gagaaggatg gcgcaattgc cgccttgcct gcgggcacgc
     6421 gtctccagcc gtcattgcca gaagccccag tggtggcgga agccgccccg gacggcctgc
     6481 tcgacctgag ccggcggggt gagaagagag cggagccgga catgagcctg cgagcgcgtc
     6541 tggtggatga ctccaggaac atcgtcagtg tgatcccctg gcctggtcag gagggcaggc
     6601 tcatcgcggc tgcgaatcac ggtctgctcg gcatggatgc tggaaccatc atggattcca
     6661 gcggcctccc ttctctggat gacctcaaac tcatggcgca gaaaaggatc aaagaacccg
     6721 cccccggcaa ggccgtcaac gcgcgcggta cgccgcctcg caacaacatc ccggaggctt
     6781 catcagcggc agcggtcgtt gaggcggaaa atccccagga gaaactgttg gagaaaatag
     6841 acgatgcgac gcggttgctc tcccttgcca gcggcgagga tgaggaagtc tgggtgctgc
     6901 tagacacagg tctcattctc gccctgaaaa ggctgggcca agccggggga ggatggcgga
     6961 tcgcctggct ggctgaggtg ccggatgatg tcgaaaatct cgaaggtgtt cttgagggtc
     7021 tcggtggaga gaatgtgatc gaatcctgcc ggctggcaca ctcagcggcc accggacacg
     7081 ttttgcttct ccgcgatgag cagcctgatg tagaggccga tgatgatgtc ctcaactctt
     7141 cccaggccag tgtttggttg ctgcaccctg cgcaagcgcc tgcgcagggc tttcttcgaa
     7201 agcttcgagc ctccaccctg cctctcaaag ggacactggc gagcacgaat agcacggggg
     7261 attatgtggt gagcgccact ggcaggagat tccgtggcgt caatctttct gaatgggagg
     7321 agggtgctga tccagcgttg gagccgtcat taatcgagcc gggagaaatc attaccgcgc
     7381 tcgggttgga tccttttgtt tttcatctgg cggtgggaac ccggattgga gaaatccttc
     7441 tttatgagca tgatgcaaaa gccggtgaat ggctgctcag ggagcgcttt ccggcgcatc
     7501 ccggagcggt gacttcgatt tgctggtcgt ccgacgggcg ctggttcgcg tcggcggacg
     7561 ataacgggct tgtgcgtttt tggaacgccg cgccaggggc gttcgcggca gtggagcaaa
     7621 gcaccgacta ccccacgctg gcttcattgg cggccgcttg gggagtggat tatgacgatg
     7681 tgggcgacat ccgcctgaaa ccaagatcag acggcatttt tcaatcctgc tgcatgggag
     7741 ccatccccaa gccgcagcca ggcattcgtg aagcgctggg caggctggat gaattgcgcg
     7801 cggtgtggga ggatcccgct ctcgagccgc tttccgccgc gcgcaagcgt catgacgcgg
     7861 cttttcagac aaccgtggag agtgtgaagg ctcttggcgt cacacccatg tggctgcatc
     7921 cggcactgaa gacggccacg tcctggctgc tcaccgccca ggcgaaagac gcgcgcgcgc
     7981 cgctggattt tgagcatccc gccaaggatt tgatctggcc gctgctcgcc ctggagcggc
     8041 tgcagaatga aggcggcaca tccgtctcgc tgcgcctcac gcacttggcc gtcatcaaac
     8101 agcgcatgga ggcggctgcg cgacccgctg gtgagaagag tgaccctttg tcgccggaca
     8161 atgttcctga ggcatggaaa gcagaccttg agtatgggca gaaactcacc ctttcattgc
     8221 tgggtgacct ggaagcaggc gaattgatgc agcacgagga tgtgctgcac ctgttcctca
     8281 cctcggaagc ctgcctgcaa cgcatcctgg acgaaaaatt tctccccaag gacgatctgc
     8341 tgaaactggc atccacagcc gaagacgaaa ggaacaatcg tctcgctttt tctctttttc
     8401 aaaaggccca tgaacgctgt tggctgagtg aagctcaaca ctgggtggat tacggactcg
     8461 tcacggaccg gctcaaggac tttgagcgct ccttcatgtg cttcggcatg gctgccgcgc
     8521 tatccagcgg ggatgaatcg gaacgcgcgc aacagatcag caacatgtcc atcgtcctga
     8581 acaaatggcg cggcacggac gcggatttca agtccgctgc ggcggatctg gcacgcattg
     8641 cttggggaat gaactccaag cagaccaacg cccggaccaa ctatctaaaa tggagggagg
     8701 gctcggacct caagaagaag gaactgctcg gattggtcga ggcagcggaa aagagcggca
     8761 gggacaaggc gccttacgag cttcaactgg ccagcctcca gtatggggct gatgatccga
     8821 atgaggccgc aaacgcacgc cgaatgatcg ccaaggcggc ggccagcctg cccgatgacg
     8881 aggacgaact gcgcgccgcc tgggaaatat tggaggggaa gtgggacacc tccaaagagc
     8941 ggagcgaaat gctggagcgg ttcgtggtgg ccgtcactgc ggccagtaac tggatggatt
     9001 ccgcgcgatt cgtcaaatca cgcattgcag ctgctcatcg ggaggaatgg gagttggcgg
     9061 atgcgatcac gctttttgag agagtggcgt ccgacagcgt cgatctcaat gcctggtgcc
     9121 acgccgagct tgccgacacc taccgccgcc agggcaggct gaacacggcg ctggaacacg
     9181 gggacaagtc catccagctc gacgccaccg gcgaccactg ggcctgggcc aggcgcgccg
     9241 aggtcaaggc ggagcttggc aggtgggagg aggcgctggc agacatcacg gaggcatcga
     9301 gactcagaat gcagagcgag gacgccaacg actacaccca ggctcacgcc ctgttgctcg
     9361 cacaaaaagg cgatttcgac ctggcgttga agctactgga acccaagctg gccaaaacgc
     9421 gagaagacga ggcaagtaat gcgggcaatg gggacgcgga ctggcttctc aatcaagcag
     9481 atgtgctggt gtggcaggcc gcggcttccg gcagggggat ccgtgttccg gagtcccagg
     9541 aactcggcag ggaggtgctc cgctggctgg atgcccgcaa gaccgcaaaa gatgctgagc
     9601 ttcagagcaa gggtctgccg cctgcgaaag ccaagccgcc ctcgctggtg cgcatccgtg
     9661 ccaacctgca tgagggcaat taccagaccg cggtgcaact ggccgaggag agacttcaaa
     9721 cctgggacca cgaggtggag gcgcacttgc atcacgccgc cgcattgtcc gccgcaggac
     9781 ggggagacca ggcgctggcg gccatcgccc gggcattgtc gcgcgagtat tacggcgggc
     9841 actccaacga caaggatcga ggcgtcatgc cgctttcgat gaatccctgg ctgctttcga
     9901 ttggcgcgcc aatcaaggac ttgatcaagc gcctggaact ttccgacggc gaggatgagg
     9961 ctgcgctggc agagcgcgag attcggattg cggaatggat cgtcaagcaa ccttacgcct
    10021 acacgacggc cctggagcgt gccgaagtca cacggctggc gcaggcttgt gtcaaccggg
    10081 ccctggaacg gtttaactac aagccatctg ccggcactcc ttcgattttg aagtctctgc
    10141 tgcccaggga tgcggagagc gggggtttga attcgagaac attgcggtga cgatgtcccg
    10201 gaaaaaagtt ctcctgcggg ttgcagggtc tccgcgcggc ggtattctcc ggcccaccga
    10261 ctaccccgtg gtgtaatggt aacacaggag attttgattc tctcgttcaa ggttcgagtc
    10321 cttgcggggt aaccaccgcg caatgacatg tcaatggccg agaaacggga ggatttgagt
    10381 gaggaggatg aggggcacct gcgccgcgcc atcgcgttgt cgcgtgaaaa aatgctcgcc
    10441 aacctcggcg gtccctttgg cgctgtgatc gcaaaagcgg gaagtgtcgt tgccgagggg
    10501 tggaaccagg tcatttcaag caaagacccc acggctcatg ccgaggtcac cgccatccgc
    10561 cttgcctcca cgcgcctggg gcatttcgtt ctcgagggct gcacgcttta ctcgagttgc
    10621 gaaccctgcc cgatgtgcct ggccgcagcg tactgggcgc gggttgaccg cgtggtctat
    10681 gccgccacgc gcgcggacgc ggctgcggga ggctttgatg acgaactgct ctaccgcgag
    10741 ctgccgttgc cgccggacca gcgcagcctg cccatgagcc aggcgcttca ttcggaggct
    10801 gcccgggttt tcgatgaatg gcgggccaag gcggacaagg tgatgtattg atccgtccat
    10861 gaacacgaca cgcgcgttgc gcgcgagccg ggccggtgac aaattcaatc ccgaatgaca
    10921 cggtttgact tcatcattgt cggcagcgga gcggccggcc tcagcgcggc cctgcatgcc
    10981 gcgaggcatg gcagcgtggc cgtgatcacc aagcgcggcg cgctggactc gaactcgaac
    11041 tgggcgcagg gcggaatcgc ctgcgtgacg agtgatgagg acagcattga gaagcatgtg
    11101 caggacacac tcgtggctgg cgctggtctt tgccgggagg acgccgtgcg cgccatcgtc
    11161 acagaaggcc ctgcgcgcat cgccgagttg gtggactggg gggtgcactt cgaccagcgc
    11221 gagtctgccg aggggcatct ggagtttgac ctgaccaagg aaggcgggca ctcgaagcgg
    11281 cgcgtgcttc acgccgcgga tgccactggc agggagattt cagaaaagct gctggccgcc
    11341 gtgcgcgcgc agccggccat caccgtgctg gagaaccact ttgccattga tctggtcacc
    11401 acggcgaagc tgggctttgt ttccgaggac cgagtgctcg gcctgcatgt gctcagggag
    11461 aacgatggca gcgtggtcac cctgcgctcg gatcgtgtca tcctggccac cggcggctgc
    11521 gggcgcgtct atctctacac caccaatccg cgcgtggcca cgggagacgg tgtggcgatg
    11581 gcctggcgcg cgggagcgag cgtggcgaac atggagttca tccagttcca ccccacctgc
    11641 ctctatcatc ctcagaagcg ctccttcctt gtcaccgagg ccatgcgcgg cgagggcgcc
    11701 cggctgattg gcgcggatgg ccgggaattc atgcaggact acgacccgcg cggctccctg
    11761 gctccgcgcg atatcgtggc gcgcgccatt gaccatgaga tcaagcggac gggaggaccg
    11821 tgcgtgtatc tcgacatctc gcacaagccc gccgatttcg tcatcagcca cttcccgacg
    11881 atccacaaag cctgccttga ggtgggcctg gacatcaccc gcgaccccat ccccgtcgtg
    11941 cccgccgcgc attaccagtg cggcggcgtc gtgacggatg tgaacggcgc cacgcgcatc
    12001 cgcggacttt gcgccgtggg cgaggtgggc tgcaccggcc tgcacggcgc caaccgcctg
    12061 gccagcaact cgctgctcga atgccttgtg ctctccaggc gcgccgtggt ccatatgatg
    12121 aaaaagctgc ccgtcggcaa agagaccgcg caggactacg aggtgcccgc gtggcagagc
    12181 ggcgacgcgg tggacaatga cgaactggtc gtcatctacc acaactggga tgaaatccgc
    12241 cgcctcatgt gggattacgt ttccattgtg cgcacaacaa aacggctcca gcgcgccgcc
    12301 gcgcgcctgc gcaacctgaa gcgcgaggtg caggagtttt actggaactt ccgcatcacc
    12361 agcgagctgc tcgagctgcg caacctggtc gagacggcct cactcatcgt cgagtgcgcc
    12421 atccgccgtc atgaatcgcg cgggctgcac tacacgctcg attttcccga aagggacgaa
    12481 agccgaccgc cgcaggacat ggttttgcgg cggtattgaa cgcggcctgc gtccgctgga
    12541 aggcgcttct gccaagctgc ttttcgcatg tgggacaggc gtcccgcctg tccgcacagg
    12601 caggatgcct gcgccccata tttgagtttc cagaagtgcc ttacaacgac cgcgccgtcg
    12661 gattctccac ccagcctgcg ggacgctggc ccgcgccttc gcgaccttca tctccgagtt
    12721 cgatcctggc ggcttcagcc agcgccagca gccttttcac cacctcaggc tccgcggcgg
    12781 agatctctcg atcttcggcg atgtcatcgc gcacattgta gagttcgagc ggcgcttgcg
    12841 cactcctgcc tttgccggag acgagctttt tctccagcgg cagatacagc ttccacggcc
    12901 cgctgcgcac ggcctggagc tggcccatgt gataataaaa aaagccgcgc tcatccgcgg
    12961 gggacttggc tccagcttcg ccgaaaagca gcgggcggat gtcgtgcccg tcaaggggat
    13021 tggctggaag tgtggcagag ccaagcgcgg caaaggtggg cagcagatcg agcatggagg
    13081 ccagctccct gcattccgtc ccggcgggga ttttgcccgg ccagcgcgcg atgcagggca
    13141 tgcgcatcgc cccctcgctc gtgttgtagc cccagccttt gtaaggcagg ttgctgccct
    13201 ggggaggatt gcgctgcggc gcgccgttgt cggaggtcca gatgacgagc gttttttcat
    13261 ccagcccgag gcgcttcaag gcggcgagga tttcgcccat ggaccagtcc agctcctcca
    13321 cactatcgcc ccattcgcca ttggcggact tgccgcgaaa ggccgggctg gagtaagcat
    13381 gctgagtgct gcccggcatg gcgtgaggca ggtaaaggaa gaaaggcttg tcctggtgcg
    13441 cctcgatgta gtggatggcc tcctccgtgt agcgcttcgt caggtagtcg cggtccactg
    13501 gcgcttcgat caccttttca tcgcgcatga gcggcagctc gggccagaca tcgggcattt
    13561 tgtccttcgt catgtcgtcg ctataaggga tgccgaagta ggagtcgaaa ccctggcgcg
    13621 tgggcagaaa ctccggctgg tcccccagat gccatttgcc gatgatggcg gtggcgtagc
    13681 ctgccgtttt gagcacctct gccaccgtgg tctcccgcga attcagcccc ttcggtgcca
    13741 cgggctgaag cacgaaggtg cccttgttgc tgacatgcag gttcacccgg cgcgggtagc
    13801 agcccgtcat ggccgcggca cggctgggtg tgcagacgcc gctggcggag taaaaactgg
    13861 tgagcttcat gccctcggcg gccatcttgt cgaggtgcgg cgtgcggtgc ttcgtcaacg
    13921 gattgaagca ggccacatcg ccattgccga gattgtcaca gaagacaaag atgatgttag
    13981 gcttgtccgc ggcgcttacg gtggcagcgg cgaggagcag gagggaggcg agtctggcga
    14041 gcatgacaaa aaggaacccc gcgcatgtca ggcttctttc accacgggtt cggaggcgtt
    14101 gatgcgcagg gcaaagaagc cggagatgac aaaggcggcc gcgcagaacc agagcgcttc
    14161 cttccagtct ccgttggcat caagatgcgc gttgatgagg gcgatgaaca ccggagagag
    14221 cgccgcgccg aagttccccc acatgttcgc ccagccaaac aagggcgccg tgtgacgtcc
    14281 gccaatgtcc tgcatcgtgg cccatacagc aggcagcccg gcatcggtaa aaaaggccac
    14341 catgcccagc gctggaacga gcagccaggg attttgtgtt tgcagcgcga gcacaaacat
    14401 gagcgcggcg gcaaagcgtg tgcccgccat cggcagcagg cggcccgctt tcagccccag
    14461 tagccgtacc gccgcatctg tcaggaagcc tccgcagaga atgccgccta tgccgattag
    14521 cagagccacg gttgaaagcg tgccgctggc ttcgtcgctc agcttgagag cctccttcag
    14581 gtagcgcggc atccaggtgg ccaggaaggc ccagcccaca ttcgtccaaa actgtgcgcc
    14641 gcacatgaac cagagattgc cgctgcgcag catcgccgcc cagggaaaac ggtgtgaatg
    14701 gcctgcggaa accgggcttt ccacaggacg tccttctttg agcagcgcac gctctgcctc
    14761 gttgcagcgc gggtgctgct cgggactttc gcgatagata cgccaaaagc cccaggcaaa
    14821 aagaatgccc agcgctccgt aggaccagcc tgcccagcgc cagttccccg tgctgctgat
    14881 gatcagcaca gtgagcactg gggctgcggc tccacccagc ctgccaccaa gggagactat
    14941 cccgctggca gtgcctctac cgctccagac cgcccatttt ctgagcaggc tgctgctcgc
    15001 gggataagcc cccgcctcag ccaagccgca tccaatgcgc gtgagcatga gcgaccacat
    15061 gccggtggca aaacctgtga gcgccgtgaa cagcgaccaa agcacgatga aaacggtcat
    15121 caaaggccgg atgccaaagc ggtcgctcag ccagcccgcc ggaacctgac ccagtgcata
    15181 ggcccagaag aaggctccga gcacccagga gagctgattg tctgtcaggg gaaactcacc
    15241 gcggaagctg gcggatttca cgatctccgc catgcagatc cggtccaaat acatcaaaaa
    15301 agccgccaga gtcgtggcgg cgagcacgga atggcgaacg cgggagggag cttcggacat
    15361 cgtgctttta cgcgcagtgc gtttgatgca ttgcaaaagc gggggacgat tatcaaagcc
    15421 aatgttctta taaagaacgc gattcctgcc ggtttcaggc tcacggcatg ccgatgaggt
    15481 tccgcaagat tccagaatcc gcccaacgca atatatcggg attgcgggtg cgcgaggcgc
    15541 ggttgtcctc cgatccgccg atgacacagg cacgactcgc cgcagctttg acggatttgg
    15601 gcatcgtcat ggatcgtgtg gccgtcgcca agattgaaac cggccagcgt tgcgtttttg
    15661 atttcgaact gaaggccttt gccgccgcgt tgaaccggcc ggtggagtgg ctgctaggca
    15721 tcggtccgcg cggcacttct ggtagtgcgg gagttgcgcg tggaaacaag tgagaatgcc
    15781 agcgtcatga gttttcgacc cgcgactttg ctgcgcacgg ccacgagccg ggcgaaatcc
    15841 gatgtggcga acaagcttgt gtccatgctg ctgcacgaga tcagccgcga gatcaccgag
    15901 gcgcatggcg cgcccgctgt gacgagcgaa tcctacgctt caagcgtgga gcgcgctttt
    15961 ggaaacgcct gtctttactg cgcaagaccc ctggagcgga acagcaccgt ggtggagcat
    16021 ctagacggca tgaaccgttt ccgtcttgga cttcatgttc ccggcaacgc ggcgctggcg
    16081 tgcaaggcct gcaacagtca gaagcgcgga gacgaccaga gggcggaatt gatcctggct
    16141 ggcacgggct gggagtcatt tttatctcac gacggcaccc gttgcggact gagctgcaaa
    16201 agttgcgctt actggaggga tatcatcccg gatgcggaag agaggcggat gtttctggcg
    16261 cagcgcagag aagctgtggc gcggtttcgc cggcactatc tggagatact ggccgcggct
    16321 tgtggattga gggagtcctt gcgcggtcaa gtgacggagt tataccgcgc ttgccaggac
    16381 ttcgcttcga aggagatttc tgctctcaag cggagagtgt tgtcatgatc ttgacccgcc
    16441 cccggtggct caataaagca ggaaatgaat ctccttgtcc gtgagcaggg cggtgtcctg
    16501 cacggcgagg agggcgccca tttgctccag gttttcccac tcggtttcgt agccttcgat
    16561 caaggggaaa tccgactcaa gcaggagagg ctcgaccatc gcgggggcgg tcttcccagc
    16621 gatttcggag gcgtttttat cctccggcat gaaaaccacc agcgcggcca gggccactgc
    16681 ggcggcggcc cagatgaggc tggcgggggc tttttcaccc ccgctccacc aggcgcgcag
    16741 ggcggccagc cagccgtgct cctgcggggt ctggcgcgcg gcgcggagca cgttctgggt
    16801 gaagtttgga cgcgctttga caggccgggc cttgccgagc agtttccaga gcgggtcgtt
    16861 ggggtttagc ggatccatga cagtttcgag ccattacaac tgcttccagg gcggggagtt
    16921 gcgcttgagg ggcatttttt tgaaaaacac gtcgggcgtg atggctccgg ctcttgatgg
    16981 agggagtgcg ttggccggat ctctcacagc gccgctttca gcagttcggc gagatggatg
    17041 gaggctgact ggccgacggt gacgacttcc gcgtgatcca gcgtttgcgg ctggatgcct
    17101 gcggcccagt tggtgagcat ggagatgccc gccacttcca tgcccagcgc gcgcgcctgg
    17161 atggcctccg gcacggtgga catgcccacg gcgtccgcgc ccagcgcgcg catcatgcgg
    17221 atctccgccg gcgtctcgta ttgcgggccg ggagtggccg cgtacacgcc ctggtgcagc
    17281 gtgatgtcca gcgccgccgc tttttccaaa aacaccgcgc gccatgcggc ggagtacacc
    17341 ccgctcatgt cgtgaaaatt cggcccgcct gtcaatggcg aggcgccgag caggttcagg
    17401 tggtcggtga tcagcatgag ttcgcccacg ccgaagcccg tgtgaatggc gcccgccgcg
    17461 ttggtgagca ccacgcgccg cacgccgagg ccgtgcatga cgcggatgcc cgccgccact
    17521 tcgtgagcac tcagcccctc atacaaatgc cgccgcccct gcgccacgag caccgcgcgg
    17581 cccgccacat gcgtcagcac caggcggccc gcgtggcccg gcaccgtgga tgccgtgagg
    17641 cccggcactt ccgcataggg gacctcggcc tccgcgccca gcgtctgcgc cacgccgccc
    17701 agaccgctgc cgagcacgat gacggtttca ggacgggcgg cttggatgcg ggcgaggctc
    17761 atgcggctga gttcatcgtg gaaatcctgc gccgcaactg ctcatgcaac tcctccacca
    17821 gcggcatggc cactccggcg gcgcgggcgc ggcgcacggg ttcgccccag atggcctcca
    17881 gctccacggc gcgcccttcc tcgaagtcaa tgagggagga gggcttgtag cgggacatgg
    17941 tgcgcgtgcg ctcgatcatg tccgggatca gtttgaaagg cacctcatgg cccagggcgc
    18001 gcgcggtggt gatgatttct gtcattagcg cgcgcacccg cgcctccagc gccgcatccg
    18061 ccaagatggc ggcggtgtcc ttgccgccct cggcgatggc gagtccgttg aaggggatgt
    18121 tccagaccag cttcttccag cgcgcggctg ccaggctcgg ttccaccacg cactcgatgc
    18181 cgcagcggcg gaactcctcc gcaatgcgcc gcgcgcgctc ccccggcgcg cggtcatgct
    18241 cgccgaggct gacgcgccct tgcgcgatgt ggcgcaccac gccgggcgcg gtgcggttga
    18301 tgcacacgaa gcacagcccg cccagcacgc gcttggcgcc gaagtgcgcg gccaggaact
    18361 cctcgttgcc cagtccattt tgcagggtca gcagcgccgt gtccttgtgc aaaagcgggg
    18421 gcagtaattc cagcaggaat tcattcgagg tggctttcat cgcgatgagc accaggtcgc
    18481 attcctccat ttcgcgagtg ctgccatagg ccggcacggc aagtgtcaga tcgcccagcg
    18541 ggctgagaat tttgagtccg tgctcccgaa cttccgccag atccgagcgc atcaaaaaac
    18601 gcacgtcatt gccatgctga gccagcctcc cgccgtagta gcagcccaca gcgcctgctc
    18661 ccacgaccgc gacgcggccc agaggggagt gtgcgggttc gtccgcttcc acggccgcca
    18721 ttcgggcacg gggggagtgt tcgagttcgt ccggcatgag attcaaaaaa gaccgggcct
    18781 ttcccgatct caatcgcaaa gaaatgtccg ccagtccggc gtccacctca cccgaagagc
    18841 tccgcctgcc cgctcagcgg aggcgcgaga ccgagtttgc ggtaggcggc aggcatggcc
    18901 acgcgtccgc ggggggtgcg tttcaggtag ccctgcatca cgaggtaggg ttcatgcaca
    18961 tcctccagcg tgctggcgtc ctcgcccacg gccacggcca cgctgcccat gccgacgggg
    19021 ccgccttcga acttgtggat gaccgcctcc aggatgcggg tgtccatttc atcgagtccg
    19081 gactcgtcaa tgtcgcgcat ggtgagggcc tggcgggcca cgtcttcatt gacgatgttt
    19141 tgcgctttca cctgcgcgta gtcccgcacc cagcgcagga ggtggttggc gatgcgcgga
    19201 gtgccgcgcg cgcggcgggc gatctcgtgc gcgccagctt cgtccatgac gacacccagc
    19261 agccgggcgg agcgcaggag gatgtgccgg agttcctccg cgctgtagta gtccagccgg
    19321 ttcgggatgc caaagcgcga gcgcatgggc gcggtgagca tgccggagcg cgtggtggcg
    19381 cccacaagag tgaaaggcgg caggtcgagc cggatagtgc gcgcttttgg cccctggtct
    19441 atgatgatgt ccaggcggaa gtcctcaatg gccggataaa gatactcctc gatgctggga
    19501 tgcaggcggt gaatttcatc aataaagaga agatcgcgct cctgaaggtt ggtgaggatg
    19561 ccggcgaggt cgcccgcgcg ctcgatttgc gggccgcttg tggtgtgcag ccgcgagccg
    19621 accgcgttgg cgatgagatt ggccagggtg gttttgccca gtccgggcgg accgcagagc
    19681 agcacatgat ccagcggctc attgcgcatt ttggccgcct cgaccatgac tagaagctgc
    19741 tccttcacct tggcctggcc gtgaaattcc gaaaaatccc cgggccttag ggccaggtca
    19801 aagggggaat cggcttcgtt cagggcgggg ttcacgtggc gttctttagc gcggtgccga
    19861 gcgcgtggca atggcgggat gggtgagcct gggcagcttg ggtgcgaagg atgcgagtta
    19921 cggaggcctt tcatgaattg accacccgct gtttgcccct actatgcgcg ccccgcccca
    19981 atgagccaga ccgccatcac accgacccgc gagcaagatt tccctgagtg gtatcagcaa
    20041 gtcgtacgcg ccgcggactt ggcggaaaat tccgaagttc gcggctgcat ggtcatcaaa
    20101 ccctggggtt acggcctgtg ggagaacatt cagcgccagc ttgacgccaa attcaaggaa
    20161 accggccacg tcaacgccta ctttcccctg ctcataccac tcagttatct ggagaaagag
    20221 gccgaacacg cggaaggttt cgccaccgag tgcgccgtgg tcacccatca ccgtctggag
    20281 gcgcaaaaac agccggatgg aaccacgaaa atgatcccca ccggcgagct ggccgagccg
    20341 ttcgtcatcc gccccacctc cgagaccatc atcggcgccg ccttcgcccg ctgggtggag
    20401 agctaccggg acctgccgct tttgatcaac cagtgggcca atgtgatgcg ctgggaaatg
    20461 cgcccccgcc tgtttctgag gaccgccgaa ttcctctggc aggaggggca caccgcccac
    20521 gaaacggccg aggaggccat ggtggagacc cggctcatgc acaaagtgta tgccgacttc
    20581 ctgcgcaacc acctcgccat cccggtcatt gccggcgaga agacggaaaa cgagcgcttc
    20641 cccggcgcgg tgaacacctt tactgtcgag gccatggtgc aggaccggaa ggccatccag
    20701 gcaggcacca gccattatct ggggcagaac tttgccaaag cagccaacat tcagttcctc
    20761 ggccgcgaca acacccgcca gctcgcccac accacgagct ggggagtcag tacccgcctc
    20821 atcggcaccc tcatcatggc gcacggggat gatgacggcg tcatcctgcc tccgcgcgtg
    20881 gctccgagcc agatcgtcat actgcctgtg actccgaagc cggacacgcg ccaggaggtc
    20941 atagatgcct gcgaggccct ggcgcaaacc ctgcgcgccc agacctttca cggtgagccg
    21001 ctgcgcgtcc atgtggacaa gcgcgacatc cccggcggcc agaaaaactg ggagtggatc
    21061 aaaaaaggcg tgcccatgcg ccttgaaatg gggccgcgcg acatcaccag ccgcagtgtc
    21121 gccgtttgcc gccgtgacca ggggccgaag gccaaggagt ttatccccaa ggaggaattc
    21181 cttcagaccg tcaccgagcg gctccaggaa attcaggacg ccctgctcgc gcgggccaca
    21241 gagttccggg atcaaaattt caaaaaactc gactctctgg atgaatttaa agccttcttt
    21301 gacgagaccg cgcccggagg gttcgccctc atgcactggg ccgggagcaa cgaggaggag
    21361 gaccgcatcg ccagggaaat gaaggtgaca atccgctgca ttcccatggg agacgagttt
    21421 gccggggaag gaaaatgctt tctcacaggc cgccccagca tacggcgggc ggtcttcgcc
    21481 agggcttatt gaggcccccg aaaactcggg gcatggggca cgggcatcct gcctgtgcga
    21541 acaggcggac actgttttcc atgcaaagca ggtttctgga gtccccccag tgggtggttc
    21601 ccgatcacac actcctcagg taaatatcgt gccgtctgca cgccaggcag ccggacttct
    21661 tcaaaacgaa gtcatcggtg accggttcct tgattttttt gccttcatcg catggccgtg
    21721 ggggggctgg ttggagggta tcggccaacg agcggcctcg tttcacctct tcggtgctgc
    21781 cgcgcaaaat ctttggttcg aaaaaataaa aatctttacc aatgaggcgc aaacctttaa
    21841 aaccgcgaaa taaatcacga aaaatacctc caacccaccc cttctagaag aagcccaagg
    21901 cctgatcttg gactgaatcg tttcggacaa accctttgcc aaccatgaaa accctccgtc
    21961 gccaccgcgt cctcattaat tccgccctcg cctgccttat ggcggcatgg cagatcggcc
    22021 agccgctcaa ggctgacacc atcaactgga ccgccggtag tgggctcaat tttgactggg
    22081 cagacggcac caactggagt tccggggtgc ccggtgcggg tgacgatgcc attttcggaa
    22141 ctccggttcc tcttgccact cccggcatca cgcttgcagc gggcagcctg gcgaattccc
    22201 tctcattcct cgacagttac agtctcactg gaggtgactt gaccctcacc agcggtggca
    22261 tccatgcgaa tcttggtcaa agcgtcacga tcaacagcct gctgagcggc aatgccggac
    22321 tcaccaagac agggggcggc tccgttcgtt ttgccaatgc ttccaatgat tacacgggcg
    22381 tcaccaccat tgccaacggc accatcatca tttcaaatcc agcggcactg ggtgcctcgc
    22441 ttagcccggt tgagatcact gacggaaaca ccactccctc caacacttcc accatagggt
    22501 tttatggcgg ttctctcgtg cttgatggct ccagtgcggg attcactttc gcgcgtgatc
    22561 tgaatctcag cggccagggt ccgatcggtg gccgtggagc cacggtcaac agcatcggca
    22621 acaacattct ttccgggacg gtgacctcct cagtcagttc gctggttccc gccacattcc
    22681 gcaatacccg catcaacagc atcaacggca ctctcgggtt gtccgggacg gtgaacgccc
    22741 aaggcacttc cgccaccacc tttctgtcgt ttggcggcat caacaccgcc ggcgttggca
    22801 actacaatct cacaggtgtc ctttccggaa ccgggagcat tgagaaatca ggtgccggca
    22861 ttctccattt caatccttcg agcacctccg gttttgcggg tacggtgcgc atttccgcct
    22921 ctgccaccgg gcagcaaagc agtgtgcgtg tcacacaagc aacagtcggc ggaaccagca
    22981 tttttggaac gaacactgtg tcaggtgacg atcccagtgc cattgatttg aatgccggtg
    23041 ttcttgagtt ccgaagcgag ggcaaccttg acttcaacag ccttccttcg ggcaaaaacg
    23101 tctatcatcg ggcttcttcc acggttttca cagggccggg tgtgggtggt gacaacatca
    23161 atggcctcac aagcctcggc accttccgcg tggctgcgaa cacgacagcc acttttaaca
    23221 gccgcaatgg atacggggtg acctttggcg gagcgtggac gcaagagtcc agcaacaaca
    23281 acaacacagt caccaataac atgggcggca cccttacctt cacgggcaat gcatggaaca
    23341 acagtgacgg cagcgaccgc accctcacca tcagcagcgc gggtaacacc gtcatcaccg
    23401 gcagcatcaa cacaagcggc gccggcatca aaacactcac aaaaaccgga ggggggaatc
    23461 tgacaatcct cggcacagcg accaccctca acgggccggt gaatgtgcag ggatctttga
    23521 caatcacgga tttccgctcg gtcaccaaca acacacagag catcacgctg ggcaacacaa
    23581 ccacgaccgt tggcaatttg ctcatcggca cgactgctgc cgccaccgcg ctggggctgg
    23641 aaaccagcag gccgatcatt ctcaatggca ccactgccgc caattccatc tacgccaacc
    23701 aggcgggcct caatccggca aaactgaacg gcgcgatcac gaagcccatc gcaggtaatg
    23761 cgacattcat cctcggtgga acaaacaccg cggacaacat catcaacaca ggcataccca
    23821 gcttggggac gagcggggtg acgaaagtgg ggccgggcac ctgggttctt gccggggaga
    23881 atctttacac gggaaccacg acaatcgtga acggcacctt gaagttgaag gccaatgccg
    23941 cttcctccac cattctggcg gccgccaacg acatcacttt cggcaactcc aacgtctatg
    24001 ccgggggcgc attcgagttt gtcggacagg acggcgtcaa caatgtgcag gctcttgatg
    24061 tgcttaccac gacatcaggc gccggcaccg tccggctcac gccgggcgtc ggcggcaccg
    24121 catctctctc atttgcctct caagccaccg gtgccgcagg cacggtcaac ttcgtcgggg
    24181 ctgacttcct gaacaataaa atcaccatca cgggagccaa cgggctgatc agccgcacca
    24241 attattggaa cggctcaaac tttgcctacc gcgaatccaa tgtgctgcgc gcgccggttt
    24301 acggaacgga tgcaggcttc accacttccg cgagcgcgct cacttcggct gccaacatgg
    24361 aaatcaccgg cagtttcgcc accaacacga tctcgataag cacgctgaaa atcgcgggca
    24421 gccgcactct gaccatcaac ccggcgcaga ccctgactct gagcgcgggt ggtgtgctgg
    24481 ttaccggcgg caatgccctt atcacaggtg gcaccgcgct ggccctgggt tcccaggctc
    24541 ttgtcgtgag aaccaacctg ccgggtgact cattggaaat tcagtcggtc gtcaccggca
    24601 cgggtggact caccaaaggt ggcgagggga cgttgattct tgcggcagcc aacaatcaga
    24661 caggcacagt gacgatcaat gagggcacga tccagctttc cggctctgga cggctgggcg
    24721 ctgcggcagc gttgaccatg cgccagggca caattctcga cctcaatggg gtcactcctt
    24781 caaccaacac caacgcctac aacaacaatg gcagtgtgac cagctccgca ccggccactt
    24841 tcactgtggg tggcgctgat ggaaccggca cttcatttgg taccgtgaat ggtgccatca
    24901 gccttaccaa ggtcggcact ggagcgcaga gctggctggg ttcgagcact tacacgggtg
    24961 taacgaccat tggctccaca gggttggtca ccgtcaatac tttggcgaac ggtggtctgc
    25021 caagcggcat tggccagtcc tcgaatgctg catccaacct tgttttcaac ggctccacag
    25081 ggggtctcgt ttaccagggc aacgttgtga atggtattct gaatcttgat tcagcctcgg
    25141 ccgccacgga ccgtcttttc accctggctg gcactggtgc cacactctcc tccacagttt
    25201 cgaacaataa cgccatcgtc tggagcagca ccggcgcgat tgtccacgcg gtcacaggtc
    25261 cgcaaagcct tgtcttcacc gggacttcca ccggcgataa cactttcaac cctcaactga
    25321 cagacagtgg ttcgggtgcg aatatcacca gtgtgaccaa aaccgcggcc ggacagtgga
    25381 acttcggcaa ccccaataac acttacaccg gcaccaccac gatttcgaac ggcgtcctcg
    25441 ctttcaacga caatggagcc ctgcccgcaa acagccctct ggctctcggt acaaccacca
    25501 ccagcggcat tcttcagatg agcggcactt ttgagagaaa catcgttgca actcctgctg
    25561 ccgggaccgg caccatctcg tggataggca caaccggcgg cggcggtttc gcggcgcatg
    25621 ccagccagct tgttgtcgcg attggcggca cgggcagccc gacagcgctg acctggggca
    25681 gtggtggttt tgtgccgagt ggcgccgcgt tgattttcaa ctccgcaagc gccctggctg
    25741 atgtggagtt ccgcaacgcc attgatttcg gagcggcggt gcgcaccatc acggtcaatg
    25801 acaacggcaa cactggcgcg gattacgcaa ctctcacggg tgacctcagc ggcgctggcg
    25861 gcggccttct gaaaaatggc gcggggattc tccggttgta tggtgtcaac tcctacaccg
    25921 gcaccaccga agtgcaggcc ggcactctcg tggtgacctc tctcggcggc agcactggcc
    25981 cggccacatc aagcgtgggc gccagcggtg tggcgatggg tgacagcaac gccatcgttc
    26041 ttggcaatgc cacaaccact ggcggccttc tgcaatatgt ggggccgggc gaaaccagcg
    26101 accgcaagat ccggctgcgc ggcaccaccg ctggcaatca aattcatgcg gatggctccg
    26161 gaccgctgat tctcaccaat gtggcccacg acactgttga gacggggagc aagactttgt
    26221 cactccgcgg aacaaacact gccggaaaca tgatcaccag ccagttgtct gacaacgtcg
    26281 ctggagtgct gagcattggt gtggatggca gtgccacttg gattctcacg aacggctcca
    26341 acaactacac aggcaccacg accgtcgggt ctggtgctct gggcattggg catgatacag
    26401 cgcttggcac tggcaacttg attgtcagca atggaaacct cttcgcgttt ggcggcgacc
    26461 gaaccattgc caacacattg cagatgaatt ccggcacttc acatggcttc ttgggggatc
    26521 acagcctgac attcacgaat gcggtcaatc tggctgcgtc aacgagcagc agcaatttta
    26581 ccaatgtgag tattgtgccc ggcgaggcgg ttattttcga tggcggagtt ttcgcggatg
    26641 ccctcaccgc cacccgcaac tggagcattg aagggcaggg cgagcttgtg atcaacggca
    26701 gctttacgac cagcactgct ttcggcgtca atatcatcaa aacgggcaac ggcaccctga
    26761 ctctcggcac cgatggctcc ggctccaact ggaatcaggc cggcaatgcg gtggatgtgg
    26821 atcgcggagt attgaaattc gccgctgata acgccatccc aactgcggcc tcaacgaatg
    26881 gaggcctgac cctgtccccg gaagtgctca ccactgacac cgccacagtg gacctcaacg
    26941 gcacgaccca gacagtcaac gccatcacga tgaacacgga tggcgtggcc atcatagaca
    27001 acacctcggc catggctgcc actctgcgct ttggagccaa tgacacggtg gccagcattg
    27061 gcggcggcat cggcacctac accgttacag acagtggggc gggggcgctc tccctggtga
    27121 agctgggcaa caccagcgcc accattccga ccggcgtcac ccttacctac cagggcggaa
    27181 ctggcgtgga aggcggaacc ttcaccatcg cctccccggt cagcggcacc agctcactct
    27241 ccgtcatcaa cagtggcagc atcctggccc tcaccggcgg cattacctcg ccgtccgcca
    27301 tcacgtcagt ggtcgtggaa aacggcggca ccctcagtct gctcgacggc gcgggcaaca
    27361 agctcgacag cctcaccaac cttcagctcg gctcctccgg cggcaccatg tccaccttga
    27421 acctcaatgt cggggacagc ttggtggcag gcgacgagtt gaacaccgac accctcgccc
    27481 tgctcactgg tggcaccttg agccttttct ccggaaacca gatcactttc aacctcaccg
    27541 acatcggtct cagtccgaac cagcaatatg tcttgctgga cgctaccgct attggcggcg
    27601 gcctcttcgg tggtccgctc aacatcgggg attacatcct cggaggaaca cctggcggct
    27661 tttccttcat cgacctgact actaactcca ccacgaacca gatcatcctt accaccggca
    27721 acctcatcct gggcacctcc tactggcgcg gcctgacgga cagcacctgg aacgcagccg
    27781 caaacaactg gtccactgac aaggccggga ccactccggc gctttcgatt cccggagccg
    27841 gtactgacgt ggtctttgcc tgggacaatc ccggcagcgg cccgctggtc actactctgg
    27901 agcagaactt caaggtcaat tcactcacct tcgaggcggg cggaaccacc acgccctcct
    27961 cggtcactat caaccccgga acagtcgcca ccaaccgcat cgagatcgcc ccgcagatgg
    28021 ccggagacgg catcgaaatc tccgccggcg gacctgccgc cgtgaccatc agcggaccgg
    28081 tccgtctcgg cggcacggcc acaagccaga cctggaccgt ggcggacgcc ggttcggtcc
    28141 tctccctcgg ctcgctggca ggggaaaggg acgttaccaa agcaggctcc ggcaaggtgg
    28201 ttctcaccac cgccgccgac ccgactttca acggaggggt cactacggat ttcaccatca
    28261 atgcgggaac actggagatc ttggatgtca gcgccctggg caacccgatc aacagcaacc
    28321 tcgccaacgt gacggtgaac aacaccggcg tcttcttcta caacggcgct gccagcacgg
    28381 cgacgaatct ggcgatgccg atcactctga atggcggcac gctctcggca ggcaatgccc
    28441 ccgcacccac taccacgatc acgcaaaact acagcggccc ggtcaacgtc agtggcaatt
    28501 cattcattaa catggccaat tccaacggcc cggcaacgaa taccgctcag aacatcaccc
    28561 tttccggcgt cgtcagtggt tcgggtggac tgacggtgtc cagcaacacc accacgctga
    28621 cggggggcaa tcctgaaagt ggaaccctcc tcatcagcaa cgctgccagc acctggtctg
    28681 gaccgctcac actcacgagt ggaaccgtgg acatcggggt ggctgccagc ccgacagtgg
    28741 tcccgagcag cgtcaccttc gatgcttttg gaagactcat cgtgcgcggg gttaacggcc
    28801 agacatacga cctggcgggg gccatgaact tcacggcagg caccatcggt gaatatttgc
    28861 tggacaacac cagcgcggtt ctcgccgctg actttgtggt taaccaggac ggccaggtca
    28921 acctcggttc cggcgggacc ggtgccagcg cgcgcttcac agtgaacgat gccgccacgc
    28981 ttctcaatat taacggcaat gtcgttcttg gcggcaatag cagcatctct gtggatggag
    29041 gcgatgcgga ctcatttgtc acgatttccg gagtcatttc tgatggcggc tccggttatt
    29101 ccctggccat taacgacgac gctggcggct gggcaatcac gaacgacatc attcgacttg
    29161 ccggattgaa caccttcacc ggcaacattt cactcgattc gggcatcctg gaattcaaca
    29221 cagtcaccgg catcagcggt ggtgccagct ctctcggcaa cggcacggcc attttgacca
    29281 caaacgccgc caatctgcgt ttcatcggat cttcagcaca gtcaaccaac cgcccgataa
    29341 acactgccgg cggcgcgttg acgttgtctg cgaatggcgc cactgccgct gacaccatca
    29401 cttacaacgg gctcatcacg gtggggtcca cagcggacgg ttctcagatc gtgctgactg
    29461 gcgcggcggg ccgcgagggc atcatcacag gcggcatcat gcagaccggc gacgccgccg
    29521 atttcacggt caatggcggt ctttggacgt tgcagacagg cacttcccgg attggtgatg
    29581 acgcgactgt gacaggtgcc gggacggttt tggatgtgga cagcggggtc ttgcaggttc
    29641 gtgatgattt cacggtcacg gccaatggtg tgctcaatct gaatgctgct ggtgtactca
    29701 ctttcaacat tgccactctc agcgcggatg cctcactgcg agccacaaat ggcggggtga
    29761 tcaacctcgg tgccagtaat gcggtggtca cgaccgaatt tgacggactc cggattggca
    29821 cggatgccgc aggcattggc actctgaaca tgaatgcgtt caaccagtct gtcaccgagt
    29881 tcattctcgg caaccgccag tctgaccgtt cgggcatcgt gaatggcaca ggtgtgctta
    29941 ccgtcaccgg aaacctggat gtttatggtg gctccatcaa tgccaacctt gccagcaccg
    30001 gaaccaacac gtttgagaaa atcagcctca acaccgtgac tttgtcgggt gacaataccg
    30061 gtctcgcctc caccggggcg agcattgttt acgaaggcac gctcatcctt gactacactt
    30121 cgagtaacac gaccaaactc cgtgccgcat ccgcattgga tatccgcggt ggacacctcc
    30181 agctcaacgg caacgccagc gccgccacca gtcagtcggt gggatcaatc accctggcca
    30241 gcggcattgg caacagcttg atcactctca atcccggtac ggggcaggac ctggttctga
    30301 atcttggagc catcacacgg gcggtcaacg cccgagcagg cacgctccgc gtcatcctgc
    30361 cttctggcac gcaaagcgcc accaacggag tcacgaccac gacagggttg accaacgggc
    30421 ttgtcggcac tgcgggatac ctcacagtgg aggacggcac tggcacttgg tttgcaacca
    30481 agagcggcaa taacatcgtc gctcttgcct ccacggcgaa aaatgacatt tccacctggg
    30541 ctgcggggga tcacatcact gacgagacca caggctttac cggcactttt gccagcgtca
    30601 gcatcaacag cctgcggttt gacgccgctg gcggctccga catcaacctt ggtggaactg
    30661 gagttcttgg catcgcaaca ggcgggcttc tcgtcaccga caacgtgggc ggcacgccct
    30721 cgctgattgg gggcacgatt ttctctggag ccctggccag caacgttccg gaactcatcc
    30781 tcacccatga cgggtccaca aatttcattc ttggctcgga cctcaggacc aacactgctt
    30841 tcgtcaaatc cggagccggc acagccgttc tctccggaaa caacgtctcg actggagacg
    30901 tgagcgttca gaacggcatt ctccaactca gcggcggcaa cgccatcggc gacacggcgc
    30961 gtgtcacact tgccaaccac cgcaacacgg tccttgagtt gcttgccagc gagacgattg
    31021 gccgcctcga aggcggacag cgccagacca acggtgactg gggtgttgtg gatgtgggca
    31081 gccataccct caccgtcaat cagactgcca attccaccta ctcgggacgc tttgccggca
    31141 ctggaacggt catcaagtcc ggtgcctcca ttatgacatt cgctggtcat gatgcggcca
    31201 atggattcac gggaacattc tcggtcgcgc agggactcgt cgtactcagt ggagccggca
    31261 gccagttgaa tggcgcatct gacattgttc tgagcggttc tggaagctct ctccgttttg
    31321 ataacgacca gaccaccgcc gtgggcagcc gcatcacgga tattgccacc atcaccctca
    31381 gcagcacggc gggaaccacc gcagattccc tcggatttta catgcgccgc actgccggca
    31441 cgaccaccgg cacggaaacg gtgggccagc tcgtccttaa cacgggacac aacacaattg
    31501 ccgcagatgg aaccgccact gaccggatcg cgcgcatcaa cttcgtgaac gcggttcccc
    31561 tcgtccgcaa caacctttcc accctgctgc tggttgcccg caactttgct gcaacagccg
    31621 gacagcgcgg acgcatttca ttctcggttg ctcccagtgg tgccatcggc gggggtggcg
    31681 cagccggcac atcaaccctc aatatctacc cgtatatggt gggtgaggat gcgtcgggag
    31741 cgcccagtgg tgctgcaaac ttcggcaaca cttttgttta ttatgagact ggcaccacgg
    31801 acatgcgtcc gctgaatctc accacggagt atgtggttgg tgaggcgagt ttcaattcgt
    31861 tgggggcggg agttcttgcg aacaatgtga gattcactgc cagcccaggc gcagcgcttg
    31921 attcggacac caccggcgtc aatgccctgg tgctggacaa tgccacgggt atcacaattg
    31981 gtggtgctgc ccagcctttg cagatcacca gcggagccat attgtccgct ggagcgggtg
    32041 ccaatgtgat caacgggttc accagcctca ccacaggcgc cgggaatccc tattacattt
    32101 atgtcacgaa tcccgcaggg accctgactc tggccgcgac cacgctcggc agctccgagg
    32161 cgctcgtgaa atctggagcg ggtgcgctca ttctgggcgc ggccaacagt gtcaccagcg
    32221 ttaacatcaa tcagggggtg ctcgaaatcg gtgacctcga ccatattggt ggtgacgctg
    32281 gggatctgcg tttcgccggc ggcacacttc gacttggcgc tggcttcacc gacgatattt
    32341 ccactcgtgc catatccttc ctgattggcg gcggcactct ggacaccaat gggattgacc
    32401 tgacactcgc aggcagtctt ggctcgggag caggtggatt caccaaggct ggtgccggca
    32461 atctgacgct caacggcaca gccacgtaca cgggcaacag tctgctttcc cttggaactg
    32521 tcacgattgg agcaaacaac gctcttggca acggcggcaa cctgacgctt gcagccggtg
    32581 ccacattgtc tttcagcgga tcacaaagcc tttcccacgg attggtcacc accagcggtg
    32641 ctagcccggc cattattggc agcggcacca tcacagcctc cagggggttc ttgttcaaca
    32701 atacaggtga catcgccatc agcgctgtat tggcgggagc gggcggcgtc ttcaaagctc
    32761 aaaccaacat gctgactctc gcgggtttga acacttatgt tggcaccacg gaggttcagg
    32821 caggcaccct cagcttcgac agcatcggta atgttggagg aggggccagt gccctgggta
    32881 atccggccac ggctcaggct ggcatcatcc gcatgggact caccacagcc gccaccacgc
    32941 tcacttacac cggcagtggt cattcctcca atcgtctcat tggcatgcag ggaaccaccg
    33001 ggggtgtgac tctcaacgcc gacggttccg gcgcgatcag ctacggcggc gccaggtttg
    33061 aaaacgccgg caataaaaat ctgacacttg gtggtgcggc tgattccgca ttgatcaact
    33121 cgatcggcgg cctcagcgag ctcggaggtg tcatgagtct catcaaaaca ggtggcagca
    33181 cttggcaact cagtgcggcc aacaaccaca gtggtgccac gactgtgtcg gagggaatac
    33241 tctccatcac aaactcaggt gctttgggca cgaccgccgc aggaacgagc gtcggtactg
    33301 gaggtgttct gcaagtgtcg aataacgtga gtgtcacagg tgaagcgttg acactcactt
    33361 caggagctgg aggcagcgcc acgctgagca acctcagtga ttccaacact tggacaggca
    33421 atttgacggt ggataccggt gctgacgcga gcaaccgcgc agtgcttaat tctgacgctg
    33481 gcaatctgct catcagcggc agcataaatc tcagctctgg cactcaagat tttgttcttc
    33541 gcggagatgg caatggtgag atcagcggtc agatcacagg ttctcagcgt ctgttcaaga
    33601 gctcggtcgg agcaggaacc tggacactta gtggagacaa cagctcgacc ttcacaggac
    33661 gagccacagt tggcaacggc gcgttgcagg tgtcctcaga agccaatctt ggtgcgacac
    33721 ctggctcttt tgaagccaat caactcaccc tcggtggtgg cagtgccaac ggagctctaa
    33781 aaacgactgc gacgatgtcc ttgagcgcaa acaggggagt cactcttgga accatgggag
    33841 gaacttttga cacggcagcg gcgacaactc tcactgtgaa cagtgtgata gccagcgcgg
    33901 cgggtgccct tgtcaaaatt gggacgggca cactgtcgct ggccggtaat aacacttatg
    33961 ttggaggcac aacagtaagc gttggaacct tactggctgt gaacaccctt ggatcggcca
    34021 ccggtgcggg accggtaaca gtggattcag gagccacact tggtggcaat ggtttcatcg
    34081 ctccaggggt ggatggtaat atcacggtca acggcacact gcaggtcggg gctgcgggtg
    34141 atgcgagcgc ccagcaactg accatcacga cgacaggggt gggcttggtg acggtgaaca
    34201 acattgtggt gttcgatctc ttcagcggcc agggcagcgg tgtgctcaat gacgcgacgc
    34261 acaacgacca gttggtggtc aacggcagca atggttttgc catcggagcg ggggcgcaac
    34321 ttcatgtgag caccagcctg ccgatagacg gcacctgggt ggcgggctca tcgtggcgtt
    34381 tgtttgactg gagcggtctg actggcggtg tgaccgggga attcagcaac ctgagcgatc
    34441 ccgctccctt caattacatg aacctgccgg acctcagttc catcggactg gcgtgggaat
    34501 actcggcggg tacgctgacg gtggtggtgc ctgagcctgg gcgcatgctg ctgctgttcc
    34561 ttggcctgat gggactcttc ttccgccgca ggcgttgatg agtccggggc acggagatgc
    34621 gggtgtaatc ggatgtgccg gtgtcctcta gcgaaggctt tccgctgccg acaacgatgt
    34681 gatggccgcc ctgcgatagc ggggcggtcg ggagaaaatc tgagaacagc gactgtctcc
    34741 gctggcctgc ctccttcata agcgcggctt ttggatggcc agtgactgtt tggggtgaag
    34801 catcgttttc catgcactgc gatggagagc ttctggatgt cattcagaga caacggatgg
    34861 actccggctg cccaagctcc aattcctacg gcggtgcttt gctgccgcca tttggctcaa
    34921 gtttttgccg gtgcatggcg acggcctttg gcgccaccag gagcgcggcc acggccatcc
    34981 aggtcatgcc cagcataacg gcaaagttca aaagccatgc cgtgatgcgt aagacggggc
    35041 tgcgcagtct ttgccaggcg ggccacgttt tctcaagtat cacgaagagt gcggcggcgg
    35101 cgccagcgaa gaactgaaaa tacacgacaa agatggagca aagatcgaaa atccagttcg
    35161 cttcgggctc ctttccgccc acttccgacc agattttcag cattcttgga atcaagacaa
    35221 gatccgcaaa gaccacaaaa aacaggcacg ggatgccata ggcggccaat tgcagatagc
    35281 tgcgcagcgc ggactcgcga ggttggaatt gtgtggatgc gggttcgctc atggcggcgg
    35341 gaatcagcgc cagtgtttct ccgcgaaggt catgtactgc tcccagtcgt agtccttcac
    35401 gtcatggccg ccggtgcgca catggtagcc gatgctgtgg ccgacgggtg tgtcaatggc
    35461 gggctgctcg ctgactccgg ttcctaattt gccgaagagt tcataaacgg ggctggcgtg
    35521 gtagccggag aggaactcgc cctttggatc ggcccactgg tcttcaaccg cgctgccgat
    35581 gtggatgggc cggggcgcgg cgagggcgac gaggaagtgc tggtccacgg ggcaggcggc
    35641 ctcgttgccg gcgtagttct tgtaggtggt tgtgaaccag tgggtgaagg cgcgtgtgat
    35701 gacggcggtg gtttcaccga agttccggcg catgagggcg gcgccgccct ctccggagtt
    35761 gtttgagatg acgaatgcga aacgctcgtc ctgcgcgccg gcccaaaggg aggtcttgcc
    35821 gagacgggag tggccgatga cggcgcaatg tttcgcgtcc acatcacggt cggtttcgag
    35881 gtagtcgaga atacggctga gaccccaggc ccaggcgctg atggcgcccc attcaccctc
    35941 cttccacctg gtggcggctc cggccgggga ggtgaggccg cgcaggccgt ccttccagcc
    36001 ctccgcgtgg tctggctcga tgtcgccata gtaggcggtg gcgacggcga agccgcggga
    36061 gaggatcatc tccagcggcc agcggctgct ttcgttgcca cggctggcct cggtggcgcg
    36121 gttgtccacg atgtgttttt ccttgctggg ccgcatccag cgggtggaga tgggcacgtc
    36181 ggtctcggtg ctgacggcgt ggtttccgcc gaagctgaga ccggcgaagc agggtgcggg
    36241 gcggttgagg ccgttcgggg tgtaaagcat gagttccatg ccctcccagg cgggacagga
    36301 gggcagggtg atctggatga gcttgcgggt ggctttgccg ccaagggcgt ctgttttcac
    36361 tgacttcaca gagaaggcgg gcgctccgag accctcgggc acgcggccat agacgtgctg
    36421 ctgatagagg gcgaggattt cgggccggcg gtgtttttgc cagtcctcag cggtcttcac
    36481 gacgctgccg tcggctcgcg tcaacagacc tggcagcgtg taagagcctg cgagggactc
    36541 gtcatagttg gccttgctct gctgggcttc ggcgaagtct tctccggtga ggatcattgc
    36601 ggtcaggagg atgggtagcg ggcgcatgga gtggaatggg atgggagcat gacaaaacgc
    36661 gagcctgccg cggcattcat caagcgcgca ggctttgctc gaattccccc aatgaccgcg
    36721 ccaggtcggc gatgcgccct gtctggctgc tcatctcctg ctcgcgccgg gcggcctctt
    36781 cttgtgcagg ggcgagcagg ggcaggaagc gggagaagag gttctcgaca tcgctgtgga
    36841 tctggcggtg cagcatgtcc cgcagcagct cggaggactc ctccaggcgg tccatcagaa
    36901 ggccccgtgt gtggttgagc gcggtctggg tgatgaggaa ggcgaagccg gcgagcagca
    36961 ggccgccgcc gcctgcgatg gccgctccga gcgggccgtc gaaccaccac gccgcaccga
    37021 tggcggcgag cacgggcagc aggagccagg gcacccagcg tgcgcggcgg cgggctttgc
    37081 gcaggccggg ttcgagcgcc tcgtcgagct ttagaccgag gacaaaacgg cgcatggtgc
    37141 tgtccatgtg gacggaaaaa agccggcgca ggtcggcatc ggcatccaca ggcagtggag
    37201 cgtcgtgggc aaaaagcgtg ccgcgtccct gaacatgaag gaagcgggcg tattgcagaa
    37261 agtcctcctc caaaatgatt cccacctggc gccagcggtc gccggtacgg tgctggagat
    37321 cctggaaaag gcggtggtcc aggctgtcct tccgatggcc gccgaagtcc tcgtcctcct
    37381 cgccgttctt ctcgaacgcg cgcttggtgg acaggacgtg ctcggccatg ccggcgacga
    37441 ggaggcagca ctcgtggtag tcgcgcccgg tggcatcaag ggcggggagg atttttttca
    37501 gggtgcggtc cacctggagt tcgcgttccg tgatgaactc ctggatgagg gcgagcgcgc
    37561 gccgagagtc ggcggctgtg gattcgacac tttggcgcag gttttccaac agctgccggg
    37621 ccaggcgtgt ggtgccggcc attttgtgga ggcgggccgc gttgtgtccg actaggcttg
    37681 agatgtgcga ttccagggga aggaagccgc tctcttccag caaccgctcg cggtcgagac
    37741 cggagctgcg cgccaggtag gcctttttgg ccgagacggc gaaaatggga aaatgctggc
    37801 cgtaacgcag gctggtgagc tgcgccatgt aatcggtgat gacctgaatc tcctcgtggc
    37861 tgcgcaaatc gcactgctgg aggatgaaaa tgacgtggcg catccaatgt ttgtgcacct
    37921 tgtcgaggaa ctgccaggcg gaggcgcccc aggggttcat ggcgctgaag acgaagatga
    37981 ccaggtccgc cacaggcacg aagcgctcgg tgatctcctg gtgctcgttt tcgatggagt
    38041 tcgtgccggg ggtgtccacg atgtggaagt cgcgcaggaa gtcgctgggc acatgcacct
    38101 cgtcgagcgt gggtgtgacg ggcacgatct gctgccggtc gccgtgcttg aaaaaaagga
    38161 tcttgtccgt ggtgggcacg actccggtgc gtgagaaatc ctggccgaaa agggcgttga
    38221 ggaaggtgga cttgcccacg ttcacctcgc cgacgacgac aaagacgaag gggtccctga
    38281 ggctggcgat gaggttttcg gcgatggcgg cagactcggc gtcaccgccg aggtcgcgga
    38341 tgagaccggc cagacgctgc agttccgcgc tcaggcgggt gcggagctgg aagtattcgt
    38401 cgccgatcat ggaagcatgg gtttgaaaaa tgccgaaagg cttcccggca gcgggccgga
    38461 gtcatcggaa ggcgtttttt ttctccaagc acagcggacg ctgtccggca agggcggata
    38521 agcggatcaa tttgtcagcc gtccggtttc gggctcgctc acgggcaggg cggggctgat
    38581 gatcatgccg acgggcatgt ttgccttctc caggttcatg agctggctga cggtgacgaa
    38641 ctggtaaccc tgggcgatga gctggtcgaa cacggcgggc atggcctcaa tggtcggagg
    38701 gtggatgtca tgcgcgagca tgatggcgcc gggccgcgcg ccgctgacca gccggttggt
    38761 gaccacggag acgccgggcc ggcgccagtc ctgcgggtcc acggaccaca tgatggtgga
    38821 gtagccgaac tcggagaaca tgagttgttt gatgcgcgtg ttgatggcgc cgtaaggcgg
    38881 gcggatgagg tgcgggcggt agttggcggc ggccatgagc gcgtcctcgc tcaacttcag
    38941 ctcgctgcgg atctgcgcgt cggagcggct ggtgaggctg gggtgggtgt aggtgtggtt
    39001 ggcgatctcg tgcccttcct caatgatgcg gcggatgatg gcggggtagg ccttggcgtt
    39061 gcggccgacg acgaagaagg tgcatttcac attacgctcc ttgcagatgt cgagcagctt
    39121 tggtgtcagt gacgggtgcg ggccgtcgtc gaaagtcatg gccaggattt tttcctgcgt
    39181 gttgaccgag gagtagctga tcttcactcc ggcaggcggg gcggtcggca gggtggaggg
    39241 ctgctgcacg cgcttggcga gcggatttgg ctgagcggat ggagccattt cctccagagg
    39301 caccgcgcgg cgcacctcgt cgaggccggc aagattttcg tctttcgagg tgcagccgga
    39361 gccgccgagc aaaatgccga accagatgct gagatacatg cgttggggag tcacgccgcg
    39421 cagtatgaga gcggcggggc atttaggaaa gcgtgaagtt tgtggaaaat gaaattcgca
    39481 tcgcctcgca ccgtggaatt gccgcgagga tgaaatacgc catcttgcgg cgttattttt
    39541 ggatcatgct ccaagcgcgc accccttcac ccccgatccc gccttctgtc agccgccgtg
    39601 gttttttcga acgtctcagc ctcggctcca tgactgccgc gcttggcgcc gagatcgtct
    39661 ttgccggcaa aatgcccgcc ggtctgatcc cggtggccct cgcgcagacc gcgccgcctg
    39721 acattgaggg aaaagacggc ctcagcatcc tcaacgaccg ccctctcgtg gccgagacgc
    39781 cggcgcacct gctcaatgat gccgtcacgc ctgagaagca tctttttgtc cgcaataacg
    39841 gcctcgctcc cgcgccggaa ctgcttgatc cggacaaatg gacattggag atcgccggag
    39901 aaagctgtga aaggccggcg gttttcacgc tcaaggagct gaagcaaaag ttcgcccacc
    39961 acaccctgcg gatggtgctc gaatgcgcgg gcaacggccg cagcgagttc aacccccccg
    40021 ccaccggcaa ccagtggacc acgggaggcg tcggctgccc ggagtggacc ggcatccgtc
    40081 ttggcgaggt gctgcggcat tgcggcatca aaaaagacgc cgtctatctc ggctaccatg
    40141 gcgcggacct ccatctctcc ggcaatccgg aaaagtccgc catctcgcgc ggagtgccca
    40201 tccagaaggc gctggaggat gagtcgctgc tcgtctttgc catgaacggc gcggacatcc
    40261 cctggcagaa cggccaccct ctgcgcctca tgttcggcgg gtgggccgcc tccacctgcg
    40321 gcaaatgggt gaaaaaaatc gtcatccgcg accgcgagca tgacggcgaa aaaatgaccg
    40381 gcaactccta ccggatgccg cgttatcctg tggcccctgg aaccaaagtc ccgcccgagg
    40441 acatggtcat cattgaggcc atgcccgtga aatcgctcgt cacctttccg aaatccggcg
    40501 ccactcatcc tgcgggtgaa aaactcgccg tgcgcggaca tgcctggacc ggcgagggga
    40561 gcgttgcctc cgtcgaggtc tccctcgact tcggctccac ctggggcagg gcggcactga
    40621 atgccccggc caaccgctac gcctggcaac agtggcgggc ggaggtgggt ttcccgaaaa
    40681 agggctacta tgaaatctgg gcgcgcgcca cggatgacca gggccgctcc cagcccatga
    40741 tcctgcccgg atggaatccc gaaggctact tgaacaactc ctgccaccgc gtcgccgtgc
    40801 aggccgtctg atccatgcgc ccgtccgccg ccattttcat actcctcacg cttcccgccg
    40861 ccgcgcagga tgctcagttg gatcccgcca ctggcatgaa aatcgcccct ggctgggaga
    40921 tcacgcgcaa ccactgcgtc atctgccact ccccgcagac tttcctgcgc cagcgcgcca
    40981 ccgaggccaa ctggacttcc gtgctcgaat ggatgcagac caacggcggc ctttggaagc
    41041 tagaacctgc ggtgcagaaa gacatcatcg cctatcttgc cgccaactac ggccccggcg
    41101 actcccaaaa ctaccgccgc gcgcccatcc cggccacgct catgcctccg aacccttatg
    41161 ccaccgaggc gcgtcttgag gctgaggaaa agaaaaagca gggcctcatt cctgccgtga
    41221 agcctcccgg tccatgaccc ccgccgcgct catcaccggt ggcaaaggcg ctctcgcagt
    41281 ggcgctgcgc gcggagttgg aagcacaggg atggcaggtc catgcgccgg gccgcgaggt
    41341 catggatgtc accgatcccg gcagtgtgag tgatttcaca ggggcgttgg aaaggctcga
    41401 cctcctggtt cacaatgccg gcctcatcct tgacgcgccg ctggcaaaaa tgagcgccgc
    41461 cagctttgac accgtgcttc aggcgcattt gcgcggcgcc tttctctgcg cccgcgccgc
    41521 cttgaggctc atggtccgca agcgcgccgg acaccttgtt ttcataggct cccgcagcgc
    41581 cctcaccgga cccgccggcc aggccaatta cgccgctgcc aaagccggcc tcatcggcct
    41641 cgcccagagc cttgccgctg aatacggccc gcgtggcatc cgcgccaatg tcgtattgcc
    41701 cggctttctg gagacaccga tgactgcaag cctgctcgcg cagcctgaga agcgcgcccg
    41761 cgtcatggcc gagcacagcc tcgggcactt gaacacctgc gctgaggcgg cacgcttcat
    41821 cgtctttctg cacacactgc cccacaccag cggccaggtc ttccatctcg acagccgcat
    41881 ccatcgctgg gcgtgacatt gtgccaggga agttggcatt tggtggcact ttgccatgcc
    41941 aaagcgtcaa agtgtcatcg ccatcgcagg tgggttatgg cgcgttttca cagcgtcgaa
    42001 agtgtattca attggttttg agccatttaa aaatacacgt tcggtgctgg catggcgcat
    42061 gccagatatc agggactaaa accctcagtt tcactttccc tccaacccaa ctctatgagc
    42121 aaaatcctcg gcattgacct cggcaccacg aactcctgca tggccgtcct cgaaggcggc
    42181 aaggccgtgg tcctcgaaaa cagcgaaggc gcgcgcacca cgccttccgt cgtcgctttc
    42241 acgaagagcg gcgaacgcgt cgtcggtcag gccgccaaac gccaggccgt caccaacccg
    42301 cgcaacaccg ttttttccgt caaacgactc atgggccgca aattcaccga actcaccgag
    42361 gcggacaaac gcatgcccta caagatcgtc gccgctgcga atggcgatgc gcatgtgcag
    42421 gtcgaagtcg gcggtgaaac gaagacttac agcccgcagg aagtcagcgc gatgatcctg
    42481 tcgaagctga aggccgacgc ggaagccaag ctcggcgaaa cgatcacgga agccgtcatc
    42541 accgtgccgg cttacttcaa cgacagccag cgcaacgcca cgaaggccgc gggcgagatc
    42601 gcgggcctca acgtgcgccg catcatcaat gagcccaccg ccgcctccct ggcctacggc
    42661 ctcgagtcga agagtgatga aaagatcgct gtctatgacc tcggcggcgg caccttcgac
    42721 atcagcgtgc tcgaaatcgg cgacggcgtc ttcgaagtgc tcgccacgga tggcgacacc
    42781 cacctgggcg gcgacgactg ggacaacacg ctcatcacct ggatcgtcaa cgagttcaaa
    42841 gccgacaccg gcattgacct cagcggccag cccgacgcca tccagcgcat caaggaagag
    42901 gcggagaagg ccaaaatcgc cctcagctcc agccagagtt acgatctcaa cctgcccttc
    42961 atcaccgctg accagaccgg gccgaagcac atcatgaaga ccctgagccg cgccaagatg
    43021 gagcagctct gcgacagcct cttcgagcgc accatgaagc ccgtgcgtga ttgcctcgcc
    43081 gccgccaagc tggacgccag caaaattgac gagctcgtgc tcgtcggcgg catgactcgc
    43141 atgcccaagg tcgtcgagac cgcgaagaag ctcgccggca aagaccccca ccagggtgtg
    43201 aacccggacg aggtcgtcgc catcggcgcg gccatccagg gcggtgtgtt gaagggcgat
    43261 gtgaaggatg tgctcctcct cgacgtgacg ccgctcacgc tcgccattga gaccgccggc
    43321 ggagtcgcca cgccgatgat cccgcgcaac acgaccatcc ccaaaaagca cagccagata
    43381 ttctccacct acgccgacca gcagcccggc gtcgaaatcg tcgtcctcca gggcgagcgc
    43441 ccgatgtccc gcgacaataa aacgctcggc accttcaagc tcgacggcat cccgccgatg
    43501 ccgcgcggcg tgccgcagat cgaggtcacc tttgacattg acgccaacgg catcctccac
    43561 gtctccgcga aggaaaagac caccggcaag gagcagaaaa tctccatcca gggcagctcc
    43621 ggcctcagcc aggacgagat cgaaaaagcc aagcgtgacg ccgaagagca cgccgaggaa
    43681 gaccgcaagc gcaaggaaag cgtcgaagtg aagaacaagg ccgacagcct cacctacgag
    43741 atcgaaaaga cccacaagga atgggaaggc aaggtccccg ccgagcagct tgcccccatc
    43801 accggcaagc tcgcctccct gaagtctgcc atcgaagcca atgacacgga agcgatgaaa
    43861 acacagaccg aggagatcga aaaactcttc gccgccgctt accaagccgc tgcagccgca
    43921 ggagccggcg cccccggcgc aggtggaatg cccgacatga gcggcatggg cggtgccgaa
    43981 gcccctgcgc cagagacgaa gagccaggac aagggcaagg tcgtggatgc cgacttcgag
    44041 gtcgtggaca aggataagta aagagggtcc gatcagttaa gtcacattga actgactcac
    44101 caaacatgaa ccggctcatt gagtttctga aatcacgttc tccagacgag agaatgggat
    44161 tgaagactat tgtcgaggcc gattcgcttc gctccgtaga cattgcagat gccttgagat
    44221 ggaaagcaag caccctgctt gaataccatt ttcaacagcc actcacctat tatcaagtcg
    44281 tccaacgtgt tgctcgcagg cttgagattc gggccgacga tggagatacg gtggaagagc
    44341 ttgagaagca aatttgctcc aaggtcttcg agacaatgtg gaataaaatg tctgatgctg
    44401 agaagcagga gtttgaaaag aaaatcaagg aggcagcaaa ggagaacgac gacaacatca
    44461 attgggtgtc tggaggtagt gtggcagctg ccatcattgc cggtaagatg tctggatttg
    44521 caatctatat cgccgccagc acagcattga gcgtattatc cactggtctt ggtctttctc
    44581 ttccgtttgt cgcctacacg acagtttcaa gagtgatttc gactgtgctg ggccctgtgg
    44641 gatggatcgg cgcaggattg cttactgcct atcagattag tggtcccgct tggcaacgag
    44701 ttacatctgc tgttgcctac atatcgatgc ttcgcaacac gccgcctcca aagcgttcat
    44761 aacctagaca acgtgatttt aaacctccag tcattgccat ccatgcaacc gcatccccgc
    44821 caaggcgcac ctatgaacgg tgaactccgt ggactgcggc agccctgctg ccgctttggg
    44881 gtgggggcag ccctgctgcc gggagtggac tcacgactgc gcgctgaact ctcggattcg
    44941 gccacccact cgcgttcgaa gccggcaggc cgtaggccgg gtgtgttcgg cgagctgagc
    45001 gccgttgtca cgctgactct ccgccgccga accgcccggc tcatgcggct ggcgcggacc
    45061 tccagtcaac ccggtgattt ggcgaagcaa acccatccgc gatcgtccgc caaagccggg
    45121 cgggctaatg ccacacccgg cctacttctg gccctgctcg cttcttttgt cctcgtctcc
    45181 tgcggcaaac aagaagctgc caaacacgct gtcgaagccc ccaagccagc cgccacatcc
    45241 ccggcccccg ttatttccga cagccgtctc gacgcggaga tcagccaggc tttgacaccg
    45301 acggcggccg agcgtgatga aggcctcgct gatcttgccg aggacatcct cgacaagtat
    45361 cccaacaaaa acgtcaccga gctgctgaat gtgcccgagg tgaacgagag cctgaaggtg
    45421 gctttgacca agctctcgga aaacaaggcg ctgcaaaacc agatcaacgg caccgtggcc
    45481 ctggccgcgc agatgcaggg tctgaatggc gagcccggca gcacgcggct ggacctggac
    45541 acctcgaatt acgaccacgc ccggaagtcc cgcctgctcc aagtggtgct ctccgaagac
    45601 ccgcggcgca tcgccggctt catcgcccag gagatcggcg aggcggtgcc ggagctgacc
    45661 tttgaaggcg tggaccgtgc cagcaatggc gtggcgatcc gtgagaacgc gcctccgcag
    45721 aaatgaacct ttttttgatg aatcccaccc cgtgaaaatt ttccgcccgg ccaccgcccc
    45781 cgcttcaggg tgtcggcggc cgggcagatg aaccaaacca aaccagaacc acaaacccgt
    45841 aagcagaaac cagaccaact atggctacct ccatcacccc gctcggtcgt cgcgtcctcg
    45901 tgaagcgctc caccagcgaa gaaaaaaccg ccggcggcat cttccttccc gacaccgcca
    45961 aggaaaaacc ccaggaagct gaagtcctcg cgctcggcac cggcaatgac gacgaaggca
    46021 aggacgtgag caccctcttc accgtgaaga agggcgacaa agtcctcatc tccaagtacg
    46081 gcggcaccga agtcaaagtg gacggcgaag acctcctcat catcaacgaa accgacatcc
    46141 tcggcatcat cggctgacgg taggacgggc atcctgcctg tcacgggtgg cccgggcatt
    46201 ctgcccggtc acgcgggctg aaagtccgct ccgcccgggc agccgaggtc gtccgcgcca
    46261 tccgcagtcc acccattacc atcacccact caccccccaa acaaacacaa cacagatatg
    46321 gctaaacaac tccacttcga cgaaaccgca cgtcaggcac tcctgcgcgg cgtcaccaaa
    46381 ctcgccaagg ccgtcaaggc cacgctcggc cccgccggac gcaacgtcat cctcgagaag
    46441 aaattcggct cccccaccat caccaaggac ggtgtcaccg tggccaagga aatcgaactt
    46501 cctgatccct acgaaaacat gggcgcccag ctcatcaagg aagtctcctc caagacctcc
    46561 gacatcgccg gcgacggcac cacgaccgcc acggtcctgg ccgaagccat ctatgccgag
    46621 ggtctccgca acgtgaccgc cggtgccaac ccgatctcgc tccagcgcgg catcatgaag
    46681 gccaccgagg ccatcgtcgc caagctcaag gaaatctcca ccccggtgaa ggacagcaag
    46741 gaagtggccc aggtcgcgac cgtgtccgcc aactgggacg ccggcatcgg caacatcatc
    46801 gccgaagcca tggacaaagt gggcaaggaa ggcaccatca ccgtcgaaga ggccaagagc
    46861 atcgaaacga ccctcgaagt cgtggaaggc atgcagttcg acaagggcta cctttccccc
    46921 tacttcgtca ccaaccctga aacgatggaa gcgaacctcg agtccgctta catcctcata
    46981 cacgagaaga agatcagcag cctgaaagac atgctgcccc tgctcgagaa ggtggcccgc
    47041 tccggcaagc ccctgctcat catcgcggaa gacgtggaag gcgaagccct cgccaccctc
    47101 gtggtgaaca agctgcgcgg caccctcaac atcgtggccg tcaaggcccc gggcttcggc
    47161 gaccgccgca aggccatgct cgaagacatc gccatcctca ccggcggccg ctgcatcacc
    47221 gaagacctcg gcatcaagct tgagaacatc gagctcgccg acctcggcca ggccaagcgc
    47281 atcaccatcg gcaaggaaaa caccgtcatc gtggaaggcg aaggcagctc cgacgccatc
    47341 cagggccgcg tgtcccagat caagcgccag attgaggaaa ccacctccga ctacgaccgc
    47401 gagaagctcc aggagcgcct cgccaagctg gccgg
//
LOCUS       DAOMYJ010000010        47003 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Prosthecobacter sp. isolate AMR_MDS_5629
            NODE_295_length_47003_cov_22.444620, whole genome shotgun sequence.
ACCESSION   DAOMYJ010000010 DAOMYJ010000000
VERSION     DAOMYJ010000010.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867850
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prosthecobacter sp. (wastewater metagenome)
  ORGANISM  Prosthecobacter sp.
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Verrucomicrobiaceae; Prosthecobacter.
REFERENCE   1  (bases 1 to 47003)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 47003)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.30
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:34:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,742
            CDSs (total)                      :: 4,684
            Genes (coding)                    :: 4,676
            CDSs (with protein)               :: 4,676
            Genes (RNA)                       :: 58
            tRNAs                             :: 55
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..47003
                     /organism="Prosthecobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_295_length_47003_cov_22.444620"
                     /isolate="AMR_MDS_5629"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1965333"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..816
                     /locus_tag="PLP58_01875"
     CDS             <1..816
                     /locus_tag="PLP58_01875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009964460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HRJ07301.1"
                     /translation="GGLVCDLEKAEVSALFAAGWTMPERFVAKGRDGHTDIHGVIVKP
                     SNFDPAKKYPVVENIYAGPHGSFAPKEFSRLVGHHVIAELGFIVVQLDGMGTNHRGKA
                     FHDVCWKNLKDAGFPDRKRWIKEAAKTRPWMDLSRVGIYGGSAGGQNAMRALLDHHDF
                     YKVAVADCGCHDNRMDKIWWNEQWMGWPVDESYVKSSNKEDAHKLQGHLLLIVGELDT
                     NVDPASTTQVVAALQKAGKTFDFMPIAGSGHGAAETPYGSRLRMEFLVRHLSP"
     gene            complement(829..1902)
                     /locus_tag="PLP58_01880"
     CDS             complement(829..1902)
                     /locus_tag="PLP58_01880"
                     /inference="COORDINATES: protein motif:HMM:NF024770.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HRJ07302.1"
                     /translation="MSTPSFLTLADQVTEHLRSEILRGRWSGALPGKHQLAAELGVNN
                     KTVEGALRQLEKTGLLLAQGAGRKRLINTRRRSTTRALRIALLLNDHVIDEKTKLLQE
                     VWHALETAGHTIIKAPKSLATLQFDPKRVAAMVRKNEADAWLIFAGSRGVLEWFASQS
                     VPAFAVFGNRAGVRIPSVSPDKPPVVAEATRHLIGLGHRRIVLLARRAVRLPQPTAGI
                     ASYLETLREHGFHAGEFNLPDWEETNAGFHECLRSLFKATPPTALIVDEVTYFVATMQ
                     FLLRHGLKVPGDVSLISTDDDVALSHCDPPAACMRWDMRPVIRRIVNWADNVSRGKAD
                     LIQTLTPAEFVPGGTIARCKATI"
     gene            complement(1899..5222)
                     /locus_tag="PLP58_01885"
     CDS             complement(1899..5222)
                     /locus_tag="PLP58_01885"
                     /inference="COORDINATES: protein motif:HMM:NF015422.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helicase associated domain-containing protein"
                     /protein_id="HRJ07303.1"
                     /translation="MMSGEAPFHEPLPDASHEAGRELLSNAPDDIEWQAMLHQLVCYR
                     IQHGHCHVPRHDRRHPKLALWLEKQMALFQKGRLSQDRQRELGALGVELDAIDDKPDR
                     ENHNEGRWEARFTELVAFKKEHGHLRITLKNQLSPGLMHWRDNQRIRFESGVLTPEQK
                     AKLDSIGFEWEPPRRISPAMEKHNVELWERMFAKLLVFREAHGHCQVPKSRKHDATLA
                     EWVRAQRDHYSKNKLMPERQRRLEEIGFAWKSDKLHFVGGWEARFAEMVAFKEKHGHL
                     RITKKSQLSPGLMHWRDNQRIRFRSGVMPPEQRAKLEALGFEWENPERLRPSMEEHNV
                     ALWESMFTRLLAFREAHGHCQVPHSKTGDQMLGKWVQRQRYHQRKNSLLPERRQRLEE
                     IGFVWHSDKLHFADAWEARFSEMVAFKEKHGHLRITKTNESSAGLSHWRDNQRIRLRS
                     GSMKPEQKARLDALGFEWEPPGWHTDVLLQIQEAQWQSKYEQLAAFKEAHGHSRVPKN
                     WKENPALGQWLQKQRSKFRKGSGAEGLLPERQEKLDALGVEWRETPPSPPHRQAFAAS
                     PADPKEKWESRYVRLVDFYRLNGHSNVPGHPDRLLRKWVLEQRKKYGEGSLEPDQITR
                     LETLSFSWVTPNSRPAVTPVQRERGPSIWDQRFAELQAFVADHGHFRVPKRNEELKTL
                     RNWVVAQRVYRRCGSLSSAHQEKLEALGFPWEPGHGGRQGNSTSSWDEHLRELIVFKE
                     RTGHTRVPHHRGPDKKLGGWLVRQRALHRQGRLPPDRVRQLDEIGVEWNPSSGRGPRN
                     RASGVSSRSSDQRMAELREFHARHGHANVPTKYPPNPLLGNWVSNTRISYRKGQLSSN
                     RIQELEELGFRWRGERNNAPILTITWEENFAMLLEFHRVHGHANVRSTDADHHALATW
                     VNSQRMKHRRGTLQADRAQKLESIGLKWGKAEYQAGLLRSWDQRFDELVAFRRQHGHP
                     HVSKADKSHRSLDIWAKTQRMYYHQGKLSADQIQRLESISFSWNGKEGFWLRQFRRWE
                     AATQRRRTRHLTAIGDADDDLAKWEQTQRNLHRLGKLSEEKAKLLTRAGLIFDPPPAP
                     KLKSPEPRHPRRGRTATPA"
     gene            complement(5209..5388)
                     /locus_tag="PLP58_01890"
     CDS             complement(5209..5388)
                     /locus_tag="PLP58_01890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ07304.1"
                     /translation="MSTPSFLTLADQVTEHLRSEILRGRWSGALPGKHQLAASSITLR
                     VAKLCSEAFHMNDEW"
     gene            5480..9982
                     /locus_tag="PLP58_01895"
     CDS             5480..9982
                     /locus_tag="PLP58_01895"
                     /inference="COORDINATES: protein motif:HMM:NF021945.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M66 family metalloprotease"
                     /protein_id="HRJ07305.1"
                     /translation="MKLLPFLAFFIATCSAGAVSIDAIYFGQTHLHKPDNPYFGTVAD
                     RDVFIKAHVTDPAAIASPAVFATLSLNGNFLTVALTGPANLPTSIPDGLGVVQHSTAN
                     TFTGVIPAAWVKKGLQVTVNVGAVQTTVTDMKVSAPTKVIMTMTDVEYFANTSGDYPA
                     GTFAELEAKWPVVDLEVRRLNNVVFRELVIPPRSDVSAQGVRIKSKAEYQGQTGLAFD
                     GEQAAALEWNAALKRAAGRSGRWSLYYLNVYGAFAGGQAGGFAGVGSGTDIGILHHEL
                     GHAMSLPHWGDNGSYPYKGAMHGISPPGGVYNQTHAGPAWAFHQPTNAFIPPTVQPGN
                     PGGKTAGTYKVDPMQGGGEGYQEAGYLMNHFSDYSVNQMRSYINSHVVVWNPALNSGN
                     GSWAQYNATTGGYTTAVSNNGVQFPTTRDAQVISILASMSGGKPGLNMVYPPIGPYTA
                     GLIRLFDPAVAADRTAAQSIYAPTSGCDYCMRVVQGGVTKTYMLAASALTSPSLTDGA
                     SLETEAINLPAADGAVTRIELLSTPNVEDVGLPANPTVFYTWGPLMPATATFDLPPEA
                     NGSSAVTMTAELGEVQYGYTGGTVEYMFTETSGNPGGTSSAWQTARSYTDTGLQPGTT
                     YTYTVSMRAGTLSSTTSAPLSATTMPVSFANSITVEDTQQFAVASGNGYKTVTGLGTF
                     DAATADKLVVVVGMEHSNNDPCSIYGVRYNGVQMIEAVHQTGGNADGAVGIYYLDNPG
                     PVGTGITVSGYNPNGGLGVAYALSGTAPGFGAFNSRRGNATTSVPITTSAANSIVVAA
                     LQNSGNNNSAGTPTANAPLTQTVGASWGSQWGSLASGYQQVATISSITPAFTTSTGGS
                     YSLNVAAVEFLAETTPASTWTQTAGGAQSWTTAANWIANVVPTTVAGDTVDFSTVDIA
                     VNTTLTLGADRAGGLWKFGDTTGTETWTVNAGNTLTLAGTTPSIDVAQNTATLNCVVA
                     GTAGLAKTGAGILALGAANTYTGGTMLSNGTLALAHASALGTGTVTLGGANNPYLNNT
                     SGSLITTSNNFVWAGNFRQGGNLTIDGNVSLTASGTVEQYSTLTINGVISGAFSITKN
                     GTGGLMLNGLNTYTGGTTARQGTVTINTIKNYGVASSLGMPASGSIVIAANNSTTLAY
                     TGTGDSTNRPIQVGGVATGAITATVANNGTGALIFTAPAFNTPVAMTGGAARTLVLGG
                     SFGTSVTPNEVQGVIADNTLNGAAAGANVVSLTKGGTGTWKISGANTYSGTTTISGGG
                     GALILGASNVLPDTTAVLLGSSTLDVDTRTDTVGTLDVTAATAAIRLGTGGAIAFADS
                     SAVDWAGGTLTITGNFVSGSSLKFGNSATALTATQLGKISSAGFTSFSLNSSGYLIAN
                     STAPTYTNWSTTNGTAGAFTADHDNDGVANGIEYFLGGNSNTTGQTPLPGVTNTSGTL
                     TITWTKSATYPGTYGTNFWIESTDTLTGTWTTETVGGNVTQNGNAFTYTFPAGTRRFV
                     RLKVDG"
     gene            complement(10064..11497)
                     /locus_tag="PLP58_01900"
     CDS             complement(10064..11497)
                     /locus_tag="PLP58_01900"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase"
                     /protein_id="HRJ07306.1"
                     /translation="MKYPTILLTLLLIGSVSHAQQKPNVLFIAVDDLNDFPTFSNRYP
                     DAKTPHMDALAKQGIVFSNAHCQAPLCGPSRASIMTSMLPDATLGFGSRQMDDKQVVE
                     RARELGTEVLHSYFAKNGYKTMSVGKLFHDGVPKGTVDAAGGDNGKRGGFRSPAGLLR
                     KNWPQKGTQTDWAAIDFPEEEFPDHVSATWAVEQIKSAHDKPFMLMVGFLRPHNPWYV
                     TQQWFDLYDRDKISLPPYRKDDFNDLPEGAEKFNVLSQMPRTDWAIEKDQWRDIVHAY
                     LACMSYADHQVGRVLDALKASAHAENTIIVLWSDHGYHMGEKNTFQKHTLWERSSHVP
                     LIFAGPGVKAGLRSHRVVSLMDVYPTLVDLCGLPANPKNEGRSLTPLIEKPDLEWPYT
                     AITEWQNGHAVQSETHRYIHYPDGGEELYDHQTDIHEWTNLAKADDPGIQAIKAKLKA
                     NLPKVTTAPEVKKPKKKKPKAPEDAKQ"
     gene            complement(11643..11912)
                     /locus_tag="PLP58_01905"
     CDS             complement(11643..11912)
                     /locus_tag="PLP58_01905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ07307.1"
                     /translation="MPFDPTKPANNSPASSAEMRSQLTSLNADMQQRATQADLANAIA
                     NALAQTSANSNGVSTLGQGADGSYNQSQMQDLINKVDELINALRR"
     gene            complement(11990..12130)
                     /locus_tag="PLP58_01910"
     CDS             complement(11990..12130)
                     /locus_tag="PLP58_01910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ07308.1"
                     /translation="MSSYALLLFPKAGVTGTFVAMSVFYPSTLPSLAAAKGHGETVRQ
                     GD"
     gene            complement(12159..12362)
                     /locus_tag="PLP58_01915"
     CDS             complement(12159..12362)
                     /locus_tag="PLP58_01915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ07309.1"
                     /translation="MTRIAKNALPDGNLVVQLVAPVWTAQQTNDIVWPSDKSGAPEIS
                     SSPPIPSSLCVPRWKSLQARYAL"
     gene            complement(12604..12990)
                     /locus_tag="PLP58_01920"
     CDS             complement(12604..12990)
                     /locus_tag="PLP58_01920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ07310.1"
                     /translation="MNTSPHQIECAIPILPVSDLARSVVFYTGPLGFKLDWGGGEDSC
                     ICSVSRDGCAIMLSQRDMISEPAWVWIGCEDESPFEMWRSNGVKVHQEPRNCSWAYEM
                     KFEDLDGNILWLGTEPRRDEPFEDQK"
     gene            complement(13014..13883)
                     /locus_tag="PLP58_01925"
     CDS             complement(13014..13883)
                     /locus_tag="PLP58_01925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ07311.1"
                     /translation="MKTRYSLLALIFVTLPLHAADPKAAWFTCDFEQPDWQTRWNMGN
                     PNLPRNLAIVPHRTTGPVKPHYGPDTPPLLAGAGKPSAGNALVITLKQGTHYGADFGF
                     DFAAQTGTEPEEAVLSYKLCFAPDFNAEAVQGGKLPGFGGIYSSDAGAAGRRVNGSDS
                     WSARGAYWKPDAKGDIPIGFYVYHADMKSDFGDTWYFSKALERGKWHDIRLAIKLNTP
                     AAKPGGKGRDNGILRGWLNGRQVFEKRDIRFRDVDTLKIRNAWFHFYHGGGQPASTDY
                     RMWIDDVVISPSN"
     gene            complement(13908..14633)
                     /locus_tag="PLP58_01930"
     CDS             complement(13908..14633)
                     /locus_tag="PLP58_01930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ07312.1"
                     /translation="MKTRTLLFSCLLGTLGLLHAQEDQQAEKKARTPGLPFIVHDEEI
                     AKQTGPEAAPHVRSYSLGFLMHGQRTDEGRQLQRTALDLISWALKSNHTPATHEDSLF
                     MHCHTSVLIARVTEAQHAIIADTVRVLKENQPDRPAAAIAPAPANSIVRIYALSRIFG
                     VADSGDATHQKERAAKTDALISQIRTSMAMARLDKTMPKLSFHGSSNSLIAKGTAAQM
                     ELITQAITAWRENTPDFRTVRGQ"
     gene            complement(14630..17692)
                     /locus_tag="PLP58_01935"
     CDS             complement(14630..17692)
                     /locus_tag="PLP58_01935"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HRJ07313.1"
                     /translation="MDKKECKHPPSFHQFTGMSDSNPTPTSTAQPQSLLEGLSPKNLM
                     AELMQPTQPGDATPAVPPLSPEELAPHFPQLEILECLGRGGMGVVYKARQKSLNRLVA
                     LKLLAPERADDPQFAARFEKEAHALAALNHTHIVGVYDFGQAGGFYFLLMEFVDGVNL
                     RQLLQTKRLTPKEALSIVPPVCEALQCAHDHGIVHRDIKPENLLIDKAGTVKIADFGI
                     AKIVHSSPLAPREEQSIPSHDAATMPFGTPDYAAPEQANGPADHRADIYSLGVVLYEM
                     LTGERPTEKLEAPSKRVQVDVRIDEIVLRALEKTPELRFATAAEFRTQVEAVTNDAGP
                     FVPPAMSASISKHSTIPRSFWIGLLVAFPVVTLLGAGLVLALGGGVSGRELIVMGAAA
                     VIPGIVIPALIFIFMPGHRPKAGVPGSPATRLPKARQAMEANLLFPLWLRLILGAAFF
                     ILLLNFAAPHVTRLGNNTQSTVTIGLSSPWLIVYPQHVGPDRIVRALAWNFRSPSFLS
                     GVFAALIACVLLAGRATVRRRLGFAMPVRPWSRGRCVLMLAVLATAMLAVTALWSVIP
                     LIKRLPTSTIIPTGSEIDRVDVTSEQAIVQTHGSKDAGLVFLFGNPAMRWTPKSLYLD
                     AMFNVTLTSGGFEPGVRWSITPRRDIRWTYQLDGPTGIMPGKLAFHPGAPAADSEGAY
                     VIGEFRPEKGVPVPIAVRLEVRRAGFPTGAHIGRDGHSVLVTHDQAISALHYVLYYQG
                     DFGTTSTGTQNLATNTWKDEGAVKLKNGRTFGYRREALHPHELHINGTRHDLREGRVF
                     ALRDDGTVEQFRFFPKLDVARDPAAVSRMIYGDEAAPEQKNAPNRLPDARILVYAATG
                     IERGWVQIQSHLDASHELAFFVGEEGSGWFHAVRFEEKGDYQFFIEKSGDIRLESGGK
                     GRGFVIRNGPGGDADQAKSTIYVSMEPGGTVPAGELVFRRGEIRVTENATRIVFADIV
                     TPGGELIPITARVRPTTPVAPGPEGATPSVPQILNPRTQTPSPTHHHP"
     gene            complement(17685..18350)
                     /locus_tag="PLP58_01940"
     CDS             complement(17685..18350)
                     /locus_tag="PLP58_01940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009962803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HRJ07314.1"
                     /translation="MCLAKADSEDGRRALADLCDAYYEPVVAYLRSVFRDADAAREMS
                     HAFFAEMLGGGAINTADRERGRFRSYLLGAVKHFVSRQREMQLSLKRGAGLQAVSMDE
                     EGVVEVRDCVIISPDAAFDRQWALTVLERSLEALRAECRSGGKERLFDHSRPFLTGDS
                     AYGDQGAAAAACGMSIEAFRVALHRMKKKLRQCMKAEITGTLEDAASVTDEMQILFEA
                     LGG"
     gene            18756..20225
                     /locus_tag="PLP58_01945"
     CDS             18756..20225
                     /locus_tag="PLP58_01945"
                     /inference="COORDINATES: protein motif:HMM:NF020976.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase D family protein"
                     /protein_id="HRJ07315.1"
                     /translation="MKLPSASSPIWSRRRFVRESAVFSATWPLACRALAVPAPATHQA
                     TGVRVGEATDHSAIVWVRLTENGERNNEGFVFPKKGKKAKPVPVNLPVKDIEGACPGM
                     AGRLRLRYGLKVDLSDAVETEWVGVSAATDFIHHFHLANLKPGSTYHYVSETAGAEGE
                     PAHRGFSGRFHTAPSPDSTTPVRFCVMTCQGYPDRDHADGHPIYPSMSALDPQFISMT
                     GDLVYYDSNEPRATSPALARLHWERMFSLPRLVDCLRKTSTYWLKDDHDTLTNDSWPG
                     QKAGELTFAEGQEIFHQQAPLGADSYRTFRWGRDLQIWFTDGRDFRSPNRMPDGPEKT
                     IWGAKQKEWFKKTVAESDATWKVLISPTPLVGPDRKGKNDNHANSGFAHEGDEIRSWL
                     RENVAGHFFVICGDRHWQYHSVHPETGVQEFSVGAASDSHAGGTPGYDAKMHRFHRVK
                     GGFLCVDVRREARESSIVFQLRAVDGTVEYEAGFQRAVA"
     gene            20361..22331
                     /locus_tag="PLP58_01950"
     CDS             20361..22331
                     /locus_tag="PLP58_01950"
                     /inference="COORDINATES: protein motif:HMM:TIGR02601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter-associated beta strand
                     repeat-containing protein"
                     /protein_id="HRJ07316.1"
                     /translation="MKPGTFCFSFALALSASLPALCQAQSPYYTDTGSPVNPPWSEIY
                     PALNFQSADPGPVNGSVIDVNNTLTEVGLFGGRPLLVPSQRIHVCCNSFTNDFPNFTR
                     WFQTDGNTQVFRLFVNDENTANDRTGTARTEAFHTSSGWNYVDGKTFEWSGRYTIARR
                     KQGYSIFQSKNADDDWSVQLNISGSGFLVINNRRHAEDVTVTNPDGSNKDFDGLGFDA
                     RILDDGRNYKVWIDGVLMADNYFDRPTSDTNFRWGMYLGSSILVPPADNSVILVSGAQ
                     VKSWTGILTSAKTAIIKANNGNGPGATTSWVGGVLPGLHNVATWDSTVTAANTISVGS
                     DQVWAGLKISNPGGNVTFNGTSLLGIDEAGVDMSTTTRNLTLNCPIELRVTAPWTVAS
                     TFTATVNSALRGYGGLTLSGGGVLTLMGANEYTGPTVINAGRLRLGNGGTTGSLSPMS
                     AISVGAGALLHFQRSNDVLQGTDFSSAPITGDGGVLKWAPGKLILTADNSYTGPTTVI
                     AGTLQLGNGGTTGKLSTNSAISFSGGTTFEVNRSNNTIQGSGFSGEAITGNVAVTQSG
                     TGKLTLTANNTYTGVTTINAGTLQVGNDGPTGSTGSGNIINNGTLRFDRTGALEVPNA
                     ISGSGQVIVDCPAAPPTPSPSAESTPSQAPLR"
     gene            22262..23182
                     /locus_tag="PLP58_01955"
     CDS             22262..23182
                     /locus_tag="PLP58_01955"
                     /inference="COORDINATES: protein motif:HMM:TIGR02601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ07317.1"
                     /translation="MPGSPADTITLSGINTFTGATSVNSGTLLVNAPGSLHADSAVTV
                     NAASLGGNGLIGGSVTIASSGRLTPGAAPGATGVLAIGGDLSVSDLAGGSGKLFFDLR
                     APNDSDSITVGGTLSMGSALLGFDDFVFTGLGGLTAGAYKLITAAGISGTLDPAHLTG
                     TLGGFNATLARNGNDLELVLETPAGFTSWQTANGASGAITGDHDNDGVPDGIEYFLGG
                     PSGNTTGQTPLPGIMNNGGTLSITWVMGPGYTGIYGTDFTIETSETLTGMWHTEPLGV
                     RVIINGSSVTYTFPVPPVTCTFVLLKVNSP"
     gene            complement(23474..24913)
                     /locus_tag="PLP58_01960"
     CDS             complement(23474..24913)
                     /locus_tag="PLP58_01960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ07318.1"
                     /translation="MIRHLSFLPAILVMILGAVLPALGERAESYSGRVVVIPVGKDDL
                     ISRARFEFMSRTLERCTAEGAEAVVFDIDTPGGLLWDTVDLMMNDLQKLKPRSFAFVN
                     KRALSAGAMIAVATDGIYMAPAGTAGAATPVYSGGVQMDDAERAKLNSATMGMARAVA
                     KCKGHDPRVIEAMIDMSKELKVGDVELDTKETILTLDADQAVMLLDGKPLFAKGIVHS
                     IDDIKKAEGLNGETVTAEPTTFEQIAIWVTMYATILIIIGVAGGYLEMQTPGFGIPGL
                     VSVAAFLLFFFGHYVAGSLVGHETAVMAGLFVLGILLVIVELMVMPGMMIPGILGFVC
                     IMVALVYTMSHWEVPVLPVVEGEPQPWKFHLAAYATGLRNFALGIIGAGVLVLVLFRF
                     LPQTAPFQKLVLAGAAGGSAEDTPVVKAARSVRVGACGVAVSDLRPYGTADFAGNRVE
                     VMVEQGYLARGTPVRVREVLGTKIIVDAA"
     gene            25092..26501
                     /locus_tag="PLP58_01965"
     CDS             25092..26501
                     /locus_tag="PLP58_01965"
                     /inference="COORDINATES: protein motif:HMM:NF014045.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HRJ07319.1"
                     /translation="MTRKSPDDMAFNTAIFYDVENLLKGYSFSSHLLNNLSLREILES
                     VDKTGLVKGIAVQRAYANWSDARLAVMRGEISELGIDPVQVFGFSKDQKKNAADIQLV
                     VDAVDLAHVRPAIEVFVIVSGDGGFAALAKKLHEYGKTVIGCAYPTAANRVLRSVCDA
                     FVPIPDPEDEREVIPLARPQPPPAANGKPPKPKAPQAAPPPPASLAAPSAQTTPAAPS
                     VHPVVRRMAAQFTRREPKDRQDMMRMTREVIEWLGREQAGALGRGLHLATVGEALRQY
                     IADFAPEKAGFAKLADFLQWVCAGTQYQVARLPGGVPAVAERAQPPADAELLPDLDET
                     WLHSPEHYRSCLRAGLPIYRLPEMEALRLAGAFLLTEKPAPMELGPLIEMVSARHADE
                     LSLESVKRALLCFHSAGFFLRDPEGAPLAEQRLSLKPELQETGDLVEPVRKAVLSKLA
                     SMLGRVDDAVFEDMLARPS"
     gene            26740..27723
                     /locus_tag="PLP58_01970"
     CDS             26740..27723
                     /locus_tag="PLP58_01970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ07320.1"
                     /translation="MSASKVLPYEDSASAREELIPFLTGAFPGGPDAARWRLRLAHWW
                     DENPFCEPGSPRGWVLRHEGRLAGFLGLIPARYAAQGAPVSALLGTSWYIVEGHRNAA
                     LPMAMQYQRQAGAHLLVETTPSPEVRAMLERAGWTGETRARRLLLPCGAVGSALVALR
                     GKAWPSLQPGRRVVTDTAEVLAVARAWQRPDRLEKWVCPASLRWYAASTMRRHLFAGV
                     VDGAGCLSSFAWLTPHHSGQARLAVLEGFTTEADDTEWRALMGAVARGEAAWPGARAW
                     ALTWLAFDTDELAAGAPFLRAGWVDVCHYHKKPPALAAPKHSVMAEGDFGL"
     gene            complement(27772..28026)
                     /locus_tag="PLP58_01975"
     CDS             complement(27772..28026)
                     /locus_tag="PLP58_01975"
                     /inference="COORDINATES: protein motif:HMM:NF012759.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantetheine-binding protein"
                     /protein_id="HRJ07321.1"
                     /translation="MTAAADTTVRLLKILRPRLRLLAAGAEIPLDADLGRLGLDSLES
                     IDLLMDIEAEFGAPVPDEKITVETFATAANLLRVIEEAAG"
     gene            complement(28030..29268)
                     /locus_tag="PLP58_01980"
     CDS             complement(28030..29268)
                     /locus_tag="PLP58_01980"
                     /inference="COORDINATES: protein motif:HMM:NF014803.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ07322.1"
                     /translation="MSDFLKALAAAHGTPLYAYDLDIVAASTAALRAELPDGARLFHS
                     FKANPLPAVARQVRLGGAVAEITSEGEMEAARLAGHDLREALFGGPGKTEAVIMEALA
                     AGVRWFSCESWTDLGRVSAAAARVDAVAQVLLRVNPAEAPSARLAMTGVESQFGFDEE
                     ALLAAGTGALAKFPGARIRGVHVYFGTQVAGVEALVENTRRALAVAERLERALGFVCA
                     VVNAGGGFPWPYANDAPPPCLTGLREGLEEAWAESPLHGTAELWFEAGRYLSAAAGTL
                     VARVLEVKHSRARRFVILDTGIHHLGGMAGLGRIPRGAVTFLNLSGAPENAAAPPADI
                     VGPLCTPLDSLARGIPLPDVRPGDLLAIPNVGAYGLTASLLGFLSHQAPLEIAHLGGE
                     VLEVWRWRTGHERLNRAALS"
     gene            29427..31199
                     /locus_tag="PLP58_01985"
     CDS             29427..31199
                     /locus_tag="PLP58_01985"
                     /inference="COORDINATES: protein motif:HMM:NF012552.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 17 protein"
                     /protein_id="HRJ07323.1"
                     /translation="MKTILLLSALSAAAALAQPFGICFGPFTGNQDPATGVVLPEAQL
                     RGRLTPLRGKFSWVRSYGSTHGLQHVGRLARGMGFKTAIGAWIGPEDTPEGIAANQAE
                     IAALIAAANAGHVDIAIVGNEVLLRGDLSEERLLEIIAEVRAAIPWWVPVTTAETHDN
                     LLAHSAVMAACDVICAHFHPYWQGIRIDEAVSWLDDEYQALRLAAGGKWCVIGEAGWP
                     SEGDPEQLAEPGLENAAYFWSAFQSWAVRRNIPNFYFSAFDEAFKEALEPNRVGKNWG
                     IHTAKGVLKPGFKNVFDDKLLPEAAVLRAAVEPPALPPPPSVALTAIPAHGVKAQKTL
                     CGVVSDLGKTAAYRRLACYIEVDGLYWTKPFFNKPLFPVLPDGAFTTKIVTGGVDQKA
                     TEIALFLVPSDYKAPLAAGVPFIPGSIEQDALASAFVSRPEGSPAGGDGEPGFTASAS
                     VVRKLIAGSASESASLKGIVVLPADFEAAGVVVGISLCGLEWSFNPLGKSGSAVASDA
                     SAAKAVGSLSLKRDAKYGFWRWSAALKRSASASLDAYGMINATISTGSPVFLPLALDL
                     DGQAFETVCRLHYTAAQNGTGTAK"
     gene            31365..32423
                     /locus_tag="PLP58_01990"
     CDS             31365..32423
                     /locus_tag="PLP58_01990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ07324.1"
                     /translation="MPCPYLPTLKVALNTLLSLWPDCPLKHARGDSLAEYLASIEKII
                     RGLGEEESAARKAGEAAQDDDVDGDMTPVHFPRLTHGVANWLDAFVFAAGHAPKDACN
                     ALTGAFDDSAGVTNLLVIYATMCRLPDEKAETDAWWLLLQRMHVVAKRRAIGALNSES
                     IAEEATSEVVKKIFEAKNNAAWPPRCAPAAWVVNSLNKAVVSLLNKAANESTRLSSLD
                     DQHDAIAEKMGGDRFPRGISDRTWLLLDSLEKVKDMEDAGNKCLTPREIEHLEWRVLY
                     LVTKKTAADRAVAALARDIVIRSLTDAGWTFRTAGLSWDKLPVTREEVAKCLGFTIET
                     EAVYWHHLRRKIRRIINP"
     gene            32470..32769
                     /locus_tag="PLP58_01995"
     CDS             32470..32769
                     /locus_tag="PLP58_01995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ07325.1"
                     /translation="MNTENDLPEDDFIETAFQELADAAAFEAAALQEARAIYTPERVR
                     DLLAQARLTAVQQPRLDQTRQTAESRAANMSVLALAASAGEPLTELKKSLETDGF"
     gene            32769..37502
                     /locus_tag="PLP58_02000"
     CDS             32769..37502
                     /locus_tag="PLP58_02000"
                     /inference="COORDINATES: protein motif:HMM:NF012618.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ07326.1"
                     /translation="MKPPLFQRWCNRFLPGFGSWRKAFRSHLSASSLTTLSADEWRDL
                     LVKECETAQKGSPGDPCSAQIQRTIQNDKWRCLLRAYPEAAIFLHDKGYEHSDSKHKA
                     ELVAECTGLLETTGLTSADASAVLVEFLGTRPDCQVRMQVVLVQESPTRAVLAELVLT
                     LVDDAVAGNSSCSFVPSPAMRLWLGWDEELQTLADDFKKSLVEAYIQACELAEWPKSC
                     HIIWSLCFSREHPFSHEHPAAPDLSGKSLQLPAAIGLSLLAARNSSVRWKAGLNRDAI
                     ARMEPRSLLATGCFGASTDDLKAKLAKFFEFTSNDRESFHAVLLPEDDNNLLHLGESP
                     GKYERTSHPRTWLCRQTKTIPIVARPGLSELLQAVVVEKMIHVSRGPGFQGRLVTPPL
                     ASSSPATAPDPVADWMEAESPRVLVIENDDADQAADALARAASKTGWTVMGYWPDSNT
                     ADPLVVVQSLVAQARRATQNSDAVPSGGAFHSDPVERELQMLAHCLDSLPEGSKALMI
                     LAGVDFISDHHGRSSLPARLAGIVRRHFNTNSELRLILQSGSYAGVPRTDDVKFLELA
                     KGKAGKGNAWNAAGLCELLLARDDHDDPLGWLVCARRLFRRDKTWRTLLGESEAESLK
                     NAIQNASAVLNVPEEDLWRMSMITRRQIMEQLCETDTGKERVKEWHCRWGRRLFMLLH
                     NEPSPQDAAGDMPAAREARQYRLDHVLGHLRLGRLWDNFHEALQNPGFILEHLAQPRG
                     VRRLAKSLEKALSGKTCLPVDHPARPRVSELAFVIHPLADHVLKIDEALARQCLHNSI
                     VEQKETGLRHWLKLFLAATVRLEQAEQACLDQAGQAWLRLLPTASGTSTLGLLPSHTL
                     RKPAPMVAAAFCPQLTAQGRPQVLTFSSDTVQLWKLGETERSFEANTHWAGASSIRQT
                     PVRMLVPGFINGRYAILVSAYGVSIFDTKNGSCQLNMVKLSFRSLTSVLHGRRLYLSV
                     ASTPPQLMVYEVRDTGDVSKPFSFAFVSEWKLSEEKMLGGDHAKKNAGGVTDEDADRD
                     IYRSLAVTLDGSLLAVAKMDGVVDVYNTPDLSLTHQDGVVDDYSVPDFSLKHQLPARD
                     RRVDSITFDPDGRFLVVGGGLKSGQVEIWDLNECRATIARVHVNSIWSLGFVRHENED
                     FLISGGYDRFVKVLSWPALLKLHQEHKPNPARQEIKDGLFNSINRHYHLHYLDLPSGV
                     VRSQRKTSDIGMSLDTVGGWLVAGAAGHPFAVYEMGELLRDPGSGVTTAPVHTAVVVG
                     APVHAPIFVWAGDDGCIYCRAGLDGAIQVHQVGLAEIHRIDVSQNWIAAATVNGVAVI
                     DRQTAAPSVIWNPTTGIRSDAVRMFKAGGRDLLVCGDCKGSVSLHDLSARNRLWSNLE
                     SDLKSKSKIRELAVRVSDDGSKIWIASCGDDSSVRISVLRFSLDSATEPVEEVATIEL
                     SDPGLKGAKAHRGRVKSLLWCGNSLVTGGHEFIKVWRLENPKGESPVFFPPLKLEGHV
                     GEIWSLDYQPELDLLASGSQDQTVRVWRLKTARLLASEPRKAPVVCVAWQKCPEAGCG
                     PILHVVDTGVSADPQCSSYELRGLR"
     gene            complement(37499..38209)
                     /locus_tag="PLP58_02005"
     CDS             complement(37499..38209)
                     /locus_tag="PLP58_02005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ07327.1"
                     /translation="MDLAFGGMRTRVVDADSDFLSRHFFLAWNPNGKMHSVSAALSNC
                     FSDPVAGCRVRSLSALAGEMTRNLEKQDWRACGQGFLDYNREFQAWSKSYLSPSVASR
                     MSSLVSRAGKDRLPGWKHPGAGDAESVLLVCDGRSETISFLRSEFQSCGWEFQPVRGQ
                     GGLQVSLDTPGQKLTVSAPHRVDFIGAADLGADARIAVEGTCLACAVGPRLQIDCPFV
                     HALQGDMRNGWSASVDRA"
     gene            complement(38503..39480)
                     /locus_tag="PLP58_02010"
     CDS             complement(38503..39480)
                     /locus_tag="PLP58_02010"
                     /inference="COORDINATES: protein motif:HMM:NF027594.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4922 domain-containing protein"
                     /protein_id="HRJ07328.1"
                     /translation="MTATSFPPQSASAADSLVDALSGFIQEKTQADFSAIRSNQSLCA
                     ALDFFWHAQIRLLDVASPSSKRLQFCAPGKPSFFASVAPDRVRRPKTSANGRCPLCDY
                     SLLSQGRQVGIAHGRFRFLINPFPFSERLFTVVRAEDGHVPQGISAACVYEVALTLGA
                     RLADMMELAAELPDFLILHNAGAGASLPHHLHYQALPRWRRVYPLEFAARHDAGYFNV
                     PSAYPVAFFVFRAMTPDSILAALREHWLDLLDVPGHAHAAASIIASGANLYVVPRDTR
                     VHASFPVACLEALGEWVFHDETSAREACHQRLWEWLASAQDQALRDLVQ"
     gene            complement(39542..40234)
                     /locus_tag="PLP58_02015"
     CDS             complement(39542..40234)
                     /locus_tag="PLP58_02015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ07329.1"
                     /translation="MREDVLTGRKDGLQASKEFALLWQESVKSLSGRVCELTRWRRPG
                     FIRGTGFLRHILAWPGMPAEFVFFKPVVREFSALMEGLLGILFAGVAAAPHDYCKHVR
                     APYSFESEDGDVMTHAYLHWQDWMKDMLDRARFLGVGCGPAQAGGSLEMRELAARLPG
                     VLSHMPEIRELCRPVEGALFDSVRSACMERLNSCWLGTAEQPATAWKFVDASFEIWRG
                     FKHDTSDDDIPF"
     gene            complement(40907..43255)
                     /locus_tag="PLP58_02020"
     CDS             complement(40907..43255)
                     /locus_tag="PLP58_02020"
                     /inference="COORDINATES: protein motif:HMM:NF015119.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010498 - proteasomal protein catabolic
                     process [Evidence IEA];
                     GO_process: GO:0019941 - modification-dependent protein
                     catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proteasome accessory factor PafA2 family
                     protein"
                     /protein_id="HRJ07330.1"
                     /translation="MKTARDPAIICGEEQELGFAAFLGGRRVSEDDIYPAFLENARRF
                     GIPNEGWHGGVFVANGGSIYRDAARGGARARGGQHLEFASAECTNPRDLRLQVRGMEK
                     LMLDILDRTLESMPGGDGIRASFLRTNCDPAAAASWGTHLNIQSSAHPDDFEHNGLVP
                     HLVTKMLLGGEGGFVPRSDPLKPGEPTYLLSPRMFHFGGHVTGGNSESARERPLICTK
                     GDRHSLHHHRLHLIGFACLLSDRAHMLDRGSTMLVARVAELTRGAAFSKFALDSPADA
                     LRVVNWDPSLRALINLRNGAQMSALDIQEAYADACERHLDQLPPWAGAFLQDWKSALQ
                     DLRHGWERAMYLQWPLKRMIFETQGAFTLPNEPALLALDMKYGAYGRHETGVFDQMAA
                     AGCLPDGQLLLDETEVHRARITPPQDTRAKLRGDAISEIIIQGPLRRGDGSHASWTSV
                     FDAHRNAALDLGQTQCLTPHWRPVFKDSPKSLLESSQSYPPMRLYELGRYTEALAELD
                     SQYPDNDHARSQRVWILARLGRASEAIETLDEHSLHMTDELLLSIDHLLVSRFLGFGP
                     RPEAPLWIRVGGNADSRRSAVFASGAGAALLRMGELDAAEQKLRMAAGSLACTRIGAR
                     ARCDLARCLAMKGALEPGFSKVVEAASVLESRGFFGDWAEFGLPTLARLAHMRGERSL
                     ALDALKRSRVMLAAFEARTALLRVRVLEARIRGVMDNEADSVFDAVNGLLSGDVPSCH
                     ASDPGLLVLEDCWAQWVSGEPLPDALHSKLPAARHDPCFWGL"
     gene            complement(43255..43464)
                     /locus_tag="PLP58_02025"
     CDS             complement(43255..43464)
                     /locus_tag="PLP58_02025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ07331.1"
                     /translation="MSQTQQTRTEQTTRAPQPCDDDDADEGILAAAAQENAQLAKASQ
                     EILKKALQGLEAGAYLQANKQKSAQ"
     gene            complement(43403..45046)
                     /locus_tag="PLP58_02030"
     CDS             complement(43403..45046)
                     /locus_tag="PLP58_02030"
                     /inference="COORDINATES: protein motif:HMM:NF015119.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010498 - proteasomal protein catabolic
                     process [Evidence IEA];
                     GO_process: GO:0019941 - modification-dependent protein
                     catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proteasome accessory factor PafA2 family
                     protein"
                     /protein_id="HRJ07332.1"
                     /translation="MPKTIGGDVELANFMTGLPADYALDVNAVSARLILAAVPGVVTA
                     IPSVHASVRAAAGQQVFPVGALDEDTPGFDTTQRGLQYYHPVDWGRLFLGNGGSCYID
                     ADHPEICVPECASALDHLTWTRAGLEICRRAMLAANAGLAAEGGGRIRLLANNSDGRG
                     NSYGSHINICVTDECWSAVCLKNGPAAAFTASAFAVLAALFGQGKAGHEAYGAPCKYQ
                     LSQRADFFNVTREFSTQWPNRGLINTRQEPHAAARHGVERMHIIPFDFTLSQSAHLMK
                     TGLLQLLFALIESCAPLPEALILRDPVHAFQRFSRDVALKAAVPAQSGSETGIIEWFE
                     RFLDLLAGWKEGFEDTVPGAGSILMHARWLSEALRARDHSGLAAHLDGWSKLGLLKSA
                     MACDPALDWSSPEIKALDHLYASLDEEGLYLQMERADKGMTFFTEADLDAAIRQPPKN
                     TRAALRARLIRVIPADHLRSLDWGEVIFDLDPGGRPVRLAMPPPWAPGLDTPAGRAGD
                     YSKLCAAVNAGASFEPHHHHEPDTTDTHRTDHPRPATLR"
     gene            complement(45076..46791)
                     /locus_tag="PLP58_02035"
     CDS             complement(45076..46791)
                     /locus_tag="PLP58_02035"
                     /inference="COORDINATES: protein motif:HMM:NF012234.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HRJ07333.1"
                     /translation="MHPSREELADVIKLATDLTDPGHPDSVEQLASCADAQARAPLLR
                     LCAAEARRQKECVRRMAAINERLTEQNKLLTGEPLIEAVFIRAAENDKAVVFARGHRQ
                     QVAVDAGVVARALKPGDGVLLAKGGAAIIGRCAASLNGCVVAGFVRWAGARMLVEFGA
                     GQKELLEVPPGALEDPAVAGIEAGSEVLCHGEARIFLELLPKAAGDHFSILKEPPSVS
                     GSDMGGMEDLYQRIKTTLCACLLHGDSARRFGLSARPGVLLAGPPGVGKTLIAKRLAA
                     ELKAGHGNCVFASVKPGEFASKWYGEAEARVRAAFKHLNTQAARGLVVLYLDEVECLG
                     RTRGSAPGGVHDDKVLACVLAELDGLQSLSNVAVIASTNRVEMLDPAFVERIGGSIIH
                     VPRPDARTAAEMLKVHLPEHMPFSPNGVERHVTRSALIETAVALLYADNAPASAIAKV
                     TFTDGKQRVVLARELVSGRLLASLADAAKRRAHDRFIQDGGGGIRVEDIEWAAHETAE
                     RMRRLLTRENIHHYLTDLPQTTQVLSVDPVMPPGAAEGPPLIVPRPIQDDDRIIQLLD
                     PESQP"
BASE COUNT         9112 a        14479 c        14627 g         8785 t
ORIGIN      
        1 ggcgggctgg tgtgcgattt ggagaaagcc gaggtctccg cgctgttcgc cgcgggctgg
       61 acaatgccgg aacgcttcgt ggcgaagggt cgcgatggcc atacggacat ccacggcgtg
      121 atcgtgaagc cctcgaactt cgatccggct aaaaagtatc ccgtcgtcga gaacatttat
      181 gccgggccgc atggttcgtt cgcgcccaaa gagttcagcc gcttggtggg gcatcatgtg
      241 atcgccgaac tcggcttcat cgtcgtgcaa ttggatggca tgggcacgaa tcatcgcggc
      301 aaggccttcc acgacgtgtg ttggaaaaat ttgaaggacg ctggctttcc ggatcggaag
      361 cggtggatca aagaagccgc aaaaacccgc ccatggatgg atctgagccg cgttggcatc
      421 tacggcggca gcgcaggcgg tcagaacgcc atgcgcgcgc tgctcgatca tcacgacttc
      481 tacaaagtcg ccgtcgctga ctgcggctgt catgacaacc gcatggacaa gatctggtgg
      541 aacgagcagt ggatgggctg gccggtggat gagtcgtatg tcaaaagctc gaacaaggag
      601 gacgcgcaca agctgcaagg tcatctgctc ctcatcgtcg gcgaactgga cacgaacgtc
      661 gatcccgcga gcacgacgca agttgtcgcc gcgctgcaaa aggccggcaa aaccttcgac
      721 ttcatgccca tcgccggcag cggccacggc gcagcggaga cgccgtatgg atcgcgtttg
      781 cgcatggagt tcctcgtgcg gcatctttcc ccgtgagaca tcgcgcgttc agatggtcgc
      841 tttgcagcgt gcgatggtgc ctccgggcac gaattcggct ggggtgaggg tttggataag
      901 gtcggctttg ccgcggctga cgttgtcggc ccagttcacg atgcgacgga tcacggggcg
      961 catgtcccag cgcatgcagg cggcgggtgg atcgcagtgt gacagggcca catcgtcgtc
     1021 ggtggaaatg agggaaacgt ctccaggcac tttcagtccg tggcgcagga gaaactgcat
     1081 ggtggcgacg aagtaagtga cctcatccac gatgagcgcg gtgggcgggg tggctttgaa
     1141 aagggagcgc aggcactcgt ggaaaccggc attggtttcc tcccagtcgg gcaggttgaa
     1201 ttctccggcg tggaagccgt gctcacgcag tgtttcaagg taggatgcga ttccggcggt
     1261 gggctgcggg aggcgcacgg cgcgtctggc gagcaaaacg atgcgtcgat ggccgagccc
     1321 gatcagatgg cgggtggcct cggcgacgac agggggcttg tccggcgaca ccgacggaat
     1381 tctgacgcca gcccggttgc cgaacacggc aaaagcgggc accgactggc tggcgaacca
     1441 ttccagcacg ccgcgcgaac cggcgaagat gagccatgca tccgcctcgt ttttgcgcac
     1501 catggcggcg acgcgcttgg gatcgaattg cagcgttgcg agagactttg gcgctttgat
     1561 gatcgtgtgt ccggcggttt cgagagcgtg ccagacctcc tgaagcagct tggttttctc
     1621 atcaatgaca tgatcgttga gcagcagcgc gatgcgcaac gcccgcgtgg tgcttcgacg
     1681 gcgcgtgttg atgagacgct tgcgccccgc gccctgcgcc aggagcaggc cggtcttttc
     1741 gagctgccgc agggcgccct cgacggtttt gttgttcacg ccgagctcgg cggcgagctg
     1801 gtgctttcca ggcagcgcac cgctccagcg tccgcgcagg atttcgctgc ggagatgctc
     1861 ggtgacctgg tcggcgaggg tgaggaagct cggagtgctc atgcaggtgt cgctgttcgc
     1921 ccacggcgtg ggtggcgtgg ttcaggagac ttcaatttag gtgctggtgg cggatcaaag
     1981 atcagtccgg cacgcgtcag gagctttgcc ttctcctcac tgagcttgcc caagcggtgg
     2041 agattgcgct gcgtttgctc ccattttgcc agatcatcgt ctgcatcgcc gatggcagtg
     2101 aggtgacgtg tgcggcggcg ctgtgtggcg gcttcccagc gtctgaactg ccgcagccaa
     2161 aagccttctt tgccattcca actgaaactg atggactcaa gccgctggat ctggtccgcg
     2221 ctgagtttgc cctgatggta atacatccgc tgagtttttg cccagatgtc gagactacga
     2281 tggcttttgt ctgccttcga aacatgtgga tgcccgtgct gccgacggaa ggccacgagt
     2341 tcgtcaaaac gctggtccca ggatcgcaac aaaccagcct gatactctgc cttcccccac
     2401 ttgaggccga tggattcgag cttctgcgct ctgtctgcct ggagcgttcc ccggcggtgc
     2461 ttcatcctct gggaattgac ccaggtggca agagcgtggt ggtctgcgtc cgtggagcgc
     2521 acattggcgt gaccatgaac tcgatgaaac tccagcaaca tcgcaaaatt ttcctcccag
     2581 gtgatggtca gtatgggggc gttgtttctt tcccccctcc agcggaatcc cagttcttcc
     2641 agctcctgga tgcggttcga gctgagctgc ccctttctgt agctgatgcg ggtgttcgag
     2701 acccagttgc ccagcaatgg attgggcgga tacttggtgg gcacattggc gtggccgtgc
     2761 cgggcatgaa actcacgcag ctcagccatg cgctggtctg agctgcggct gctaacacca
     2821 cttgcacggt tccgcggccc tctgccgctg gaagggttcc attccacacc gatctcgtcc
     2881 aactgccgca cgcgatctgg cggcaggcgt ccctggcgat gcagcgcacg ctggcgcacc
     2941 agccagccgc ccaatttttt gtcgggtccc ctgtgatgtg gcacccgcgt gtgacctgtg
     3001 cgttccttga agacgatgag ttcccgaaga tgctcatccc agctactggt ggagtttcct
     3061 tgcctgccac cgtgcccagg ctcccacgga aatcctaaag cctccagttt ctcctggtgc
     3121 gctgacgaga ggctgccaca gcgccgatac accctctgtg caacgaccca gtttcgcagt
     3181 gttttaagtt cttcattcct cttgggcacg cggaaatgcc catgatcggc cacaaaagcc
     3241 tgcaactcgg cgaaacgctg gtcccagatg ctcggtcccc tttccctctg aactggcgta
     3301 acagccggac gcgaattcgg tgtcacccat gagaagctca gcgtctccag gcgggtgatc
     3361 tggtctggct ccagacttcc ctcgccgtat tttttccgct gctccagcac ccatttgcgc
     3421 aggagccggt ctggatggcc tggaacattc gaatggccgt ttagccggta aaaatcaacc
     3481 aggcggacat agcggctctc ccacttttcc ttggggtcgg caggtgaagc tgcaaatgct
     3541 tgacggtgag gcggtgaagg cggcgtctcg cgccattcca caccgagcgc gtccagtttc
     3601 tcctgccgct ccggcagcaa accttccgca cctgatccct tgcggaactt gcttcgctgc
     3661 ttttgcagcc actgccccag cgcaggattc tctttccagt ttttcggcac acgagagtgc
     3721 ccgtgcgcct ctttgaacgc ggccagttgc tcatacttgc tctgccactg ggcttcctgg
     3781 atctgtagca ggacatctgt atgccagccg ggcggttccc attcaaagcc gagtgcgtcg
     3841 agcctcgcct tctgctcagg tttcatgctc ccgctgcgga ggcggatgcg ttgattgtcg
     3901 cgccagtgag acaacccggc cgaggactcg ttggttttgg tgattcgaag gtgtccgtgt
     3961 ttttctttga aggccaccat ctccgagaaa cgcgcctccc aagcatctgc gaagtgcagt
     4021 ttgtccgaat gccacacgaa gccgatctcc tccagccgct gccgacgctc tggcagcagc
     4081 gagttcttcc gctggtgata gcgctgccgc tgtacccact tcccaagcat ctggtcccca
     4141 gtcttgctgt gtggcacctg acaatgtccg tgtgcttcgc gaaacgccag gagcctggtg
     4201 aacatgctct cccacagcgc cacgttgtgc tcttccatgg aggggcgtag ccgttcagga
     4261 ttttcccact caaagccaag ggcttcgagc ttcgctctct gctccggcgg catcacaccg
     4321 ctgcgaaagc gaatgcgttg attatcccgc cagtgcatga gtccgggaga caactggctc
     4381 ttcttggtga tgcgcaaatg cccgtgtttt tctttaaagg ccaccatctc cgcaaaacgc
     4441 gcctcccatc cgccgacgaa gtgcagcttg tcggatttcc aggcaaagcc aatctcctcc
     4501 agccggcgct gccgctcagg catgagtttg ttcttgctgt aatgatcacg ctgagccctc
     4561 acccattcag ccagtgtagc atcgtgtttc cgagacttgg gaacctggca atgaccatgt
     4621 gcttcgcgaa aaaccagcag cttggcaaac atgcgctccc acaactccac attgtgcttc
     4681 tccattgcgg ggctgattcg tcttggaggc tcccattcaa agccgatcga atcgagtttc
     4741 gccttctgtt ccggcgtcaa gacgcctgac tcgaaccgga tgcgttgatt gtcccgccag
     4801 tgcatgagcc cgggagataa ttggttcttc agcgtgatcc gcagatgccc gtgctctttc
     4861 ttaaaagcca cgagctcggt gaatcttgcc tcccagcggc cttcattgtg gttttcgcgg
     4921 tcgggtttgt catctatggc atccaactcc acgccgagcg cccccagctc ccgctgccga
     4981 tcttgcgaaa gcctgccctt ctgaaaaagc gccatctgtt tctcaagcca taacgccagc
     5041 ttgggatgtc tgcggtcgtg cctgggaaca tggcagtgcc catgctgaat acggtagcag
     5101 accagttggt gaagcatggc ctgccattcg atgtcatccg gcgcattcga cagcaactcc
     5161 cggccagcct cgtgagaagc atctggcagt ggttcatgaa atggggcttc accactcatc
     5221 attcatatga aacgcttctg agcacagttt tgcaacacgc agggttattg aactggcagc
     5281 gagctggtgc tttcccggca gcgcaccgct ccagcgtccg cgcaggattt cgctgcgaag
     5341 atgctcggtg acctgatcgg cgagggtgag gaagctcggt gtgctcacgg ggcgaacttg
     5401 gccgtttggt ggccaggggg caaatggaga atggattgcc gggagggcgc gaaattcaga
     5461 gaatgaaggc accccaacca tgaaattgct accctttctc gctttcttca tcgcgacctg
     5521 ctcggcgggc gcggtgtcga ttgatgcgat ttatttcggc cagactcatc tgcacaagcc
     5581 ggacaatccg tatttcggca cggtggcgga tcgcgatgtg tttatcaaag cgcatgtgac
     5641 ggacccggcg gcgattgcgt cgcctgcggt gttcgcgacg ctgagtttga atggcaattt
     5701 cctcacggtg gcgctcacgg ggccggcgaa tctgccgacc tcgatcccgg atgggctggg
     5761 cgtggtgcag cacagcacgg caaacacgtt caccggggtg attcccgccg cctgggtgaa
     5821 aaaggggctt caagtgacgg tgaatgtcgg cgcggtgcag accacggtga cggacatgaa
     5881 ggtgagcgcg ccgacgaagg tcatcatgac gatgacggac gtggaatatt ttgcgaacac
     5941 gagcggtgat tacccggcgg gcacctttgc cgagctggag gcgaagtggc cggtggtgga
     6001 cctggaggtg cggcgtctga acaatgtggt gtttcgcgag ctggtgatcc cgccgcggtc
     6061 ggatgtcagc gcacaaggcg tgcgcatcaa atcaaaggcg gagtatcagg ggcagacagg
     6121 cctggccttt gatggcgagc aggcggccgc cttggagtgg aatgcagcgc tgaagcgcgc
     6181 ggcgggtcgt tccgggcgct ggagcttgta ctacctgaac gtgtatggcg ccttcgcggg
     6241 cggtcaggcg ggtggttttg cgggtgtggg cagcggcacg gacatcggca tcctgcatca
     6301 cgagctaggt catgcgatgt cgctgccgca ttggggcgac aacggctctt atccctacaa
     6361 aggcgcgatg catggcatct cgcctccagg tggcgtttac aatcaaacgc acgcaggccc
     6421 ggcgtgggcg tttcatcagc cgacgaatgc tttcatcccg ccgacggtgc agcctggcaa
     6481 tccgggcgga aaaactgcgg gcacttacaa ggtcgatccg atgcagggcg ggggtgaggg
     6541 ctatcaggag gcgggttatc tcatgaatca cttctcagac tactcggtga atcagatgcg
     6601 cagctacatc aacagccatg tcgtggtctg gaatcccgct ctcaatagcg gcaacggcag
     6661 ttgggcgcag tacaatgcca ccaccggcgg ctacacgaca gcggtgtcga acaacggcgt
     6721 gcagttcccg acgacgcgcg atgcgcaggt catcagcatt cttgcctcca tgtccggcgg
     6781 caaaccggga ctcaacatgg tgtatccgcc gatcggaccc tacacggcgg gtctgatccg
     6841 gttgtttgat cctgccgtgg cggcggatcg cacggcagcg cagtcgatct atgctccgac
     6901 aagcggctgt gactactgca tgcgtgtggt gcagggcggt gtgacaaaga cgtacatgct
     6961 cgccgcatcg gcgctgacct cgccttcgct cacagatggt gcgagcctgg agacggaagc
     7021 catcaatctg cctgccgccg atggcgcggt gacacgcatc gaactgctct cgactccgaa
     7081 tgtggaagac gtgggcctgc ccgcgaatcc gacggtgttt tacacatggg ggccgctgat
     7141 gcctgccacg gccacctttg acctgccacc ggaggcgaat ggctccagtg ccgtcacgat
     7201 gaccgctgaa cttggcgagg tgcaatatgg ctacactggc ggcacggtgg aatacatgtt
     7261 caccgagacc tcggggaatc ccggcggcac atcgagcgcg tggcagacgg cgcgcagcta
     7321 cacggacacc ggtttgcagc cgggcacgac ttacacttac accgtctcga tgcgtgcggg
     7381 cacgctcagc agcacgacct ccgcgccgct gtccgccacc accatgccag tgagcttcgc
     7441 caacagcatc acagtggagg acacgcagca gttcgctgtg gcgagcggaa atggctacaa
     7501 aaccgtgacc ggcctcggca ccttcgatgc ggccacggcg gataaattgg tcgtcgtcgt
     7561 cggcatggag cacagcaaca acgatccctg ctccatctat ggcgtgcgct acaacggtgt
     7621 gcagatgatc gaggccgtgc atcaaaccgg gggcaacgcg gatggtgcgg tcggcattta
     7681 ctatctcgac aatcccggcc cggtgggcac cggcatcacc gtctcgggat acaatccaaa
     7741 tggcggcctc ggcgtggcgt atgcgctctc tggcacggcg ccgggctttg gtgctttcaa
     7801 cagcagacgg ggcaatgcca cgacctccgt gccgatcacg acttctgcgg cgaactccat
     7861 cgtcgtcgcc gcattgcaaa attccggcaa caacaacagc gcgggcacac ccaccgcgaa
     7921 tgcgccgctc acgcaaacag tcggcgcatc ctggggcagc cagtggggca gcctggcatc
     7981 gggctatcag caggtcgcca cgatcagttc catcacaccc gccttcacca ccagcacggg
     8041 cggcagctac agcctgaatg tcgccgccgt cgagtttttg gccgaaacaa ctcccgcgag
     8101 cacctggaca caaaccgccg gtggagcgca aagctggacc acggcggcga attggatcgc
     8161 caatgtcgtc ccgacaacgg tggcaggcga cacggtggac ttcagtaccg tggacatcgc
     8221 cgtgaatacc acgcttacgc tcggcgcgga ccgcgccggc gggttgtgga agtttgggga
     8281 caccaccggc accgaaacat ggaccgtgaa tgccggaaac acgctcacac tcgccggaac
     8341 gacgcccagc atcgacgtcg cgcaaaacac cgccacgctc aattgcgtcg tcgcaggcac
     8401 cgcaggcctc gcgaagaccg gcgcgggcat tttggccctc ggtgctgcca atacctacac
     8461 cggcggcacg atgctgagca acggcacgct cgcactcgcc cacgcgtctg ctttgggcac
     8521 cggcaccgtc acactcggag gcgcaaacaa cccctatctc aacaacacca gcggcagcct
     8581 catcaccacg agcaacaact tcgtctgggc cggcaatttc cgccagggag gcaatctcac
     8641 gatcgatggc aacgtctccc tcaccgccag tggcacggtg gagcaataca gcacgcttac
     8701 catcaacggc gtcatcagcg gagccttcag catcaccaaa aacggcaccg gaggtctcat
     8761 gctcaacggc cttaacacct acaccggcgg caccacagcc cgacaaggca ccgtcaccat
     8821 caacacgatc aaaaactacg gcgtcgccag ctcgctcggc atgcctgcca gcggcagcat
     8881 cgtcatcgcg gccaacaact ccaccacact cgcctacacc ggcaccggcg atagcaccaa
     8941 ccgtcccatc caagtcggcg gcgtcgccac cggagccatc accgccaccg tcgccaacaa
     9001 cggcaccggt gcgcttatct tcaccgcccc cgctttcaat accccagtcg ccatgaccgg
     9061 cggcgcggca cgcacccttg tgctaggcgg cagctttggc acttccgtca cgccgaacga
     9121 agtgcaaggc gtcatcgcgg acaacaccct caatggagcc gcagcaggtg cgaacgtggt
     9181 aagcctgacc aaaggcggca ccggcacctg gaaaatctcc ggtgcgaaca cttacagcgg
     9241 caccacgacc atcagcgggg gcggaggcgc actcatcctc ggagccagca atgtactgcc
     9301 agacaccacc gccgtgctgc tcggcagttc caccctcgat gtggacacgc gcaccgacac
     9361 cgtcggcacg ctcgatgtga ccgcagccac cgccgcgatt cgccttggca ctggcggagc
     9421 cattgccttc gccgacagca gcgccgtgga ttgggccggc ggcacactca ccatcaccgg
     9481 caacttcgtc agcggcagct cgctcaagtt tggcaacagc gccaccgccc tcaccgccac
     9541 acaactcggc aaaatcagct ccgcaggctt cacgagcttc tcgttaaaca gcagcggcta
     9601 tctcatcgcg aactccaccg cacccaccta caccaattgg agcaccacga atggcaccgc
     9661 cggagccttc accgccgacc acgacaacga cggcgtcgcc aacggcatcg aatacttcct
     9721 cggaggcaac agcaacacca ccggccagac tcccttgccc ggcgtcacca acaccagcgg
     9781 cacgctcacc atcacctgga ccaaatccgc cacctacccc ggcacctacg gcaccaactt
     9841 ctggatcgaa tccaccgaca ccctcaccgg cacctggacc accgaaactg ttggcggaaa
     9901 cgtcacgcaa aacggcaacg ccttcaccta caccttcccc gcaggcacaa gacgattcgt
     9961 gcggctgaag gtggatggct gatgaaattg ggattcaccg cttacttcga gaaggaatcg
    10021 gagatttagc caaaatggca tgaaccaccg tgaaaagttc ttttcactgc tttgcgtcct
    10081 ccggggcttt gggtttcttc ttcttcggtt ttttgacctc gggtgccgtt gtgaccttgg
    10141 gcaaattcgc tttcagcttc gctttgatgg cttggatgcc cggatcatct gccttggcca
    10201 gattggtcca ttcatggatg tctgtttggt gatcgtagag ctcctcgccg ccatccgggt
    10261 agtggatgta gcggtgcgtt tcgctctgca ctgcatgacc attctgccac tccgtgatgg
    10321 cggtgtaggg ccactcaaga tcaggttttt caatgagcgg agtcagactg cgcccttcgt
    10381 ttttcggatt ggccggcagg ccgcacagat cgaccagcgt ggggtaaaca tccatcaagc
    10441 tgacgacccg gtggctgcgc aggcccgcct tcacgccggg gccggcaaag atcagcggca
    10501 catgggaact gcgctcccag agcgtgtgct tttggaaggt gttcttttct cccatgtgat
    10561 agccatgatc cgaccataag acgatgatcg tgttctcggc atgagcactg gctttgagcg
    10621 catcgaggac gcgtccgacc tgatggtccg catagctcat gcaagcgagg taggcgtgca
    10681 cgatatcgcg ccactgatcc ttctcgatgg cccagtccgt gcgaggcatc tgtgacagga
    10741 cgttgaactt ttccgctccc tcaggcaggt cattgaagtc gtctttgcga tagggcggca
    10801 ggctgatttt gtcccggtca tagaggtcga accattgctg cgtgacatac cagggattgt
    10861 gcgggcgcag aaagccgacc atcagcatga agggcttgtc gtgcgcggac ttgatctgct
    10921 ccacggccca tgttgcagac acatgatccg ggaactcctc ctcggggaaa tcaatcgcgg
    10981 cccagtcggt ctgagtgccc ttctgcggcc aatttttcct gagcagacca gcgggcgatc
    11041 tgaaaccgcc gcgcttgcca ttgtcaccgc ctgcggcatc caccgtgcct ttgggcacgc
    11101 cgtcgtggaa gagttttccc acggacatgg ttttgtagcc gtttttggcg aagtaggaat
    11161 gcagcacctc cgtgccgagt tcacgggcac gctccaccac ctgcttgtca tccatctgtc
    11221 ggctgccgaa gcccagagtc gcgtcaggca gcatgctggt catgatgctg gcccgcgacg
    11281 gcccgcagag cggtgcctgg cagtgggcat tgctgaagac gatgccctgc ttcgccagcg
    11341 catccatgtg gggcgtcttc gcgtctggat acctgttgga gaaggtgggg aagtcattga
    11401 gatcatcgac agcgatgaag aggacattcg gtttctgctg tgcatgactg acggagccaa
    11461 tcagcaacag ggtgaggagg atcgttgggt atttcataga ggtctgcttc gagccgtgaa
    11521 cgactcttgc tgactcatca tgcatttttt gaaagcccta cctggccttg ttccggccag
    11581 gtggcggata tgggatgcat ggtcggaaag agcgactgtc tccaggccgg cctggccgtg
    11641 cgctaccgcc tcagtgcatt gatcagctca tccaccttgt tgatgaggtc ttgcatctgc
    11701 gactggttgt agctgccatc ggccccttgg ccgagggtgc tcacgccgtt gctgttggcg
    11761 ctcgtttggg ccagcgcatt cgcgatggcg ttagcgagat cggcttgggt ggctcgctgc
    11821 tgcatgtcgg cattgagcga ggtgagttgc gagcgcatct cggcgctgga ggcaggcgag
    11881 ttgttcgcgg gcttggttgg atcgaagggc atggcgtttc agaagacaag gggactggga
    11941 gactgggaga tgaggagaca cttccagaga ccgctgcggg agcgatgagt cagtctcctt
    12001 gtctcaccgt ctccccatgg cctttcgcag cggccagcga aggcagcgta cttgggtaga
    12061 agacggacat ggcgacgaag gtgccggtga cgccggcttt tggaaacagc aagagagcgt
    12121 agctgctcat gccgctggat gaccaagtga gcgtgaagct atagcgcgta tctagcttgc
    12181 aggcttttcc acctcggcac gcacagcgag gaaggaatcg gtggtgatga acttatctcg
    12241 ggcgcgccgg acttgtcgct gggccagacg atgtcattgg tttgttgagc cgtccagacg
    12301 ggggccacga gctgcacgac gagattgcca tccggcagtg cgtttttggc gatgcgagtc
    12361 acaaaggact ggaactgcgc gtcgcccgag cgccgggcag cacgaagagt gtgcgccacc
    12421 cttgatcggg aggtggcgcc ggcttgtgga tgacgaagtg gcgcatcttc tcatcgcccc
    12481 cggaacgcaa gtcgtgggac tgcatgtccg agatgtcagg cgcatcggtg gcgcgagtgg
    12541 gcgatgcagc agacgccaaa agcatcgctg ccatgaagag tgcgcgaggg actgagttca
    12601 tggtcatttt tggtcctcga aaggctcgtc acgccgtggc tccgtgccca gccagaggat
    12661 gtttccgtcg aggtcttcaa acttcatctc ataggcccag gagcagttcc tgggttcctg
    12721 gtgaaccttc actccgtttg atctccacat ctcgaagggc gactcgtctt cacagccgat
    12781 ccatacccac gcaggttcgg aaatcatgtc gcgctgcgaa agcatgatgg cgcaaccatc
    12841 tctcgaaaca gaacagatgc aggagtcttc cccgccgccc cagtcgagct tgaagccgag
    12901 cgggccggtg tagaatacca cgctgcgggc gaggtcgctg acgggcagga tggggatggc
    12961 gcattcgatt tggtgcggag aggtgttcat ggaaggatga tttggatgga cactcagttt
    13021 gagggcgaga tcaccacgtc gtcgatccac atccggtagt cggtggaggc tggctggccg
    13081 ccgccgtggt agaaatggaa ccaggcattg cgaatcttga gcgtgtccac gtcgcggaag
    13141 cggatgtcgc gcttctcgaa gacctggcgg ccattcagcc atcctcgcag aatgccgttg
    13201 tcgcggcctt taccgccggg cttggctgcg ggtgtgttca gtttgatcgc gaggcggatg
    13261 tcgtgccatt tgccgcgttc gagcgccttg gagaaatacc aggtgtcgcc gaagtcgctt
    13321 ttcatgtcgg cgtgatagac gtagaagccg atggggatgt cgcctttggc atcgggcttc
    13381 caataagcac cacgggcaga ccacgagtcg ctgccgttga cacgcctgcc cgccgcaccg
    13441 gcatcgctgc tgtagatgcc gccgaagccg ggcagcttcc cgccttgcac ggcctcggcg
    13501 ttgaaatcgg gagcaaaaca gagcttgtag gacagaacgg cttcctcggg ttcagtgccg
    13561 gtttgcgcgg cgaagtcgaa gccgaagtcc gctccgtagt gcgtgccctg tttgagcgtg
    13621 atgacgagcg cgttgcctgc ggaaggtttt cctgctccgg ccagcagtgg tggcgtgtcc
    13681 ggcccatagt gcggcttgac cggccctgtg gtgcgatgcg gcacgatggc gaggtttcgc
    13741 ggcagattgg gattgcccat gttccagcga gtctgccagt cgggctgctc gaagtcgcag
    13801 gtgaaccagg cagccttcgg atcggcggcg tgaagcggca gcgtgacgaa gataagtgcg
    13861 agcaacgaat agcgcgtttt catgatgagc gtggatggag tttcttgtta ctgaccgcgg
    13921 acggtgcgga aatccggcgt gttctcccgc caggccgtga tggcctgggt gatgagttcc
    13981 atctgggccg ccgtgccctt ggcgataagg ctgttggagg aaccgtgaaa actgagtttc
    14041 ggcatggttt tgtcgagtct ggccatggcc atgctggtgc gaatctggct gatcaaggca
    14101 tccgttttgg cggcgcgctc cttctggtgg gtcgcatcgc ctgaatcagc gactccaaaa
    14161 attcgactga gtgcgtaaat gcgaacgatg gaattggctg gagcaggtgc gatggctgcg
    14221 gccggcctgt caggctgatt ctccttcaat actcggacag tgtcggcaat gatggcgtgc
    14281 tgagcttcgg tgactcgggc gatgagcaca ctggtatggc agtgcatgaa caagctgtct
    14341 tcgtgagtcg cgggtgtgtg gttggacttc agcgcccagg agatcagatc gagggcggtt
    14401 cgttgcaatt gtctcccctc atcggtcctt tggccatgca tgagaaaacc gagcgagtat
    14461 gatcgcacat gcggcgctgc ttccggcccg gtctgcttgg cgatttcttc gtcatggacg
    14521 atgaacggca gccctggcgt gcgggccttc ttctctgcct gttggtcttc ctgggcgtga
    14581 agaagtccta acgtgccgag caggcacgag aagaggaggg tgcgtgtttt catgggtggt
    14641 gatgggttgg gctgggtgtt tgcgtgcggg gattcaggat ctgtggaaca gatggcgtcg
    14701 cgccctcggg acccggcgcc acaggcgtcg tcggacgcac tcgagcggtt attgggatta
    14761 gctcgccacc gggcgtcacg atgtcggcga agacgatgcg cgtggcgttc tcggtcacgc
    14821 ggatttcgcc tctgcgaaag acgagttcgc ccgccggcac ggtgccgcct ggctccatgg
    14881 aaacatagat ggtgctcttc gcctgatcgg catcgccgcc cgggccattg cggatcacga
    14941 aaccgcggcc ttttccgccg ctttccagcc tgatgtcgcc ggacttttcg atgaagaact
    15001 ggtaatcgcc cttctcttca aaacgcacgg cgtgaaacca gccgcttccc tcttcaccga
    15061 cgaagaaagc gagttcgtga ctcgcatcaa gatgcgactg gatttgaacc cagccgcgct
    15121 cgatgcccgt tgcggcatag acgaggatgc gcgcgtcggg cagcctattc ggggcgttct
    15181 tctgctccgg ggcggcttca tcaccataaa tcatgcgcga taccgccgca ggatcgcgtg
    15241 ccacatcgag cttcgggaaa aaacgaaact gctcgacggt gccgtcatct ctcagggcaa
    15301 agacgcggcc ttcgcgcaga tcgtggcgtg tgccgttgat gtgcagttcg tggggatgca
    15361 gcgcttcgcg ccgatagccg aaggtgcgcc cgttcttcag cttcaccgca ccttcatcct
    15421 tccatgtgtt agtcgcgagg ttttgtgtgc cggtggacgt ggtgccaaag tcgccttggt
    15481 agtagagaac gtagtgcagc gccgaaatcg cctgatcgtg tgtcaccagc accgagtggc
    15541 catcgcggcc aatgtgcgca ccggtgggga atccggcacg ccgcacctcc agccgcacag
    15601 cgataggaac aggcacaccc ttttcaggcc ggaactcgcc aatgacatag gcaccctcgg
    15661 agtcggccgc gggagcgccg ggatggaaag caagcttgcc cggcataatg cccgtcgggc
    15721 cgtccagttg ataggtccat cggatgtcgc gtctgggtgt gatactccag cgcacaccgg
    15781 gctcgaaacc gcccgaggtg agggtgacat tgaacatggc atcgagatag agtgacttcg
    15841 gcgtccagcg catggccggg tttccaaaca ggaagacaag gcccgcatct ttggaaccgt
    15901 gcgtctggac aatcgcttgc tcgctggtga catcgactcg gtcgatctcg gaaccagtgg
    15961 ggatgatggt ggacgtgggc agccgtttga tgagcgggat gacagaccac agcgcggtga
    16021 cggccagcat ggcggtggca agcacagcga gcatcaaaac gcacctgccc cggctccacg
    16081 ggcgaaccgg catcgcgaac ccaaggcgac ggcgcacggt ggcgcgtccc gccagcagaa
    16141 cacacgcgat gagggcggcg aaaacgccgc tgagaaagga cggcgagcgg aagttccacg
    16201 ccagcgcacg gacgatgcgg tctgggccga catgttgtgg ataaacgatg agccacggac
    16261 tcgagaggcc gatggtgacg gtcgattggg tgttgttgcc caggcgggtg acatgagggg
    16321 ccgcgaagtt caacaggaga atgaagaatg ccgcgcccag aatcaggcga agccacaacg
    16381 gaaacagaag gttagcttcc atcgcctgcc gtgcctttgg caagcgagtg gctggagagc
    16441 ctggcacacc ggccttgggg cgatgtccag gcatgaaaat gaaaataagc gccggaatca
    16501 cgatgcccgg aatcacggcg gcagccccca tcacgatcag ctcacgccct gaaacacctc
    16561 cacccaaagc cagcaccaat cccgctccca aaagcgttac gacaggaaat gcaacaagca
    16621 gaccgatcca gaaactgcgt gggatggtcg aatgcttgga aatcgacgct gacatcgcgg
    16681 gcggcacaaa tggaccagca tcatttgtca ccgcctccac ctgcgtgcgg aactccgcgg
    16741 cggtggcgaa gcgcagctcc ggcgtctttt ccagcgcacg cagcacgatt tcatcaatgc
    16801 gcacgtccac ctgcacgcgc ttggaggggg cttcgagttt ttccgtggga cgctcgccgg
    16861 tgagcatctc gtaaagcacc acgccgaggc tgtagatgtc cgcgcggtgg tcggcggggc
    16921 cgtttgcttg ctcgggggcg gcgtaatcgg gagtgccgaa aggcatcgtt gccgcatcgt
    16981 gagagggaat gctttgctcc tctcgcggag cgagagggct actatggacg atcttcgcga
    17041 tgccgaagtc ggcgatcttc accgtgcctg ctttatcaat gagcaggttc tccggcttga
    17101 tgtcgcgatg cacgatgccg tgatcgtggg cgcattgcag ggcttcacag acgggcggca
    17161 cgatgctgag cgcttctttc ggtgtgaggc gcttcgtttg cagaagctgg cggagattca
    17221 caccatcgac gaactccatg agcaggaagt aaaaaccacc cgcctgcccg aagtcataca
    17281 cgccgacgat gtgtgtgtga ttcagcgctg cgagcgcatg agcttccttt tcaaaccgtg
    17341 cggcgaactg cggatcgtcc gcgcgctccg gcgcgagcag tttcagcgcg acgaggcgat
    17401 tcagcgactt ttgccgtgcc ttatagacga cacccatgcc gccacgaccg aggcattcga
    17461 ggatttcgag ctgcgggaag tgcggggcga gttcttcggg gctgagcggc ggcacggcgg
    17521 gcgtagcatc ccctggctgc gtgggctgca tcagctctgc catgaggttt ttcggcgaaa
    17581 gaccttccag caggctctgg ggctgcgcgg tggaggtcgg ggtggggttg gagtcgctca
    17641 tgcctgtaaa ctgatgaaac gacggcggat gcttacactc ttttttatcc acccagggcc
    17701 tcaaagagga tttgcatctc atccgtcacg ctggcggcgt cctccagtgt cccggtgatc
    17761 tccgccttca tgcactgccg gagcttcttt ttcatccggt gcaaggccac gcggaatgcc
    17821 tcgatgctca tcccacaggc agcagcagcc gctccttgat cgccgtaagc cgaatctccg
    17881 gtgagaaagg gccgcgagtg atcaaagagg cgctctttgc ctccagaacg acattccgcc
    17941 cgaagagcct ccaaactgcg ctccagcact gtgagcgccc attggcggtc aaaagcggca
    18001 tcgggtgaga tgatgacgca gtcacgcacc tcgaccaccc cttcctcatc catcgaaaca
    18061 gcctgcaaac ctgcgccgcg cttcaaactg agctgcatct cccgctggcg cgagacgaaa
    18121 tgcttcaccg cacccagcaa gtaagagcga aagcgtcccc gctcacgatc tgcggtgttg
    18181 atggctccac cgccgagcat ctccgcaaag aacgcatgac tcatctcccg cgccgcgtcc
    18241 gcatcacgga acacgctgcg aagataagcg acgacgggtt cataataggc atcacagagg
    18301 tccgcgagcg ctctgcgtcc atcttccgag tccgcctttg ctagacacac gcgtgtccac
    18361 cgcgtggtat gaaacggggc agcgtgaagt ttcagttgag gagtggatgt catcgtcgcg
    18421 ggctatgctg ccacagttcc atgacgtttt ggaagggaaa ggtgacgggg gattttgcct
    18481 cctgaatcac atcctccgcc gcatgagcat cccgcagcag cccggtcaca agcgtgctgt
    18541 agtgcggctt cgataacgat cccggcattc ctatcgacct actccttggt gctcgctccc
    18601 agcctgcgcg tttcgccgcg gacatcggga ggtgctttgt cagccaatcg tgatgctcag
    18661 cagcccgggg cagcgagcac tcaggcccag tcgctgcatt tcagcgatag aggcggccgg
    18721 ggtgcgtgtg ttaaacagag gatcttttta ccgccatgaa attgccatcc gcctcttctc
    18781 ccatctggtc acgtcgccgg ttcgtgcgcg aaagcgctgt gttttccgcc acctggccgc
    18841 tggcctgccg cgctctcgcc gtgcctgctc cggcgactca ccaagcgacc ggggtgaggg
    18901 tgggcgaggc gacggatcac tccgccatcg tgtgggtgcg gctcactgaa aacggggagc
    18961 gcaacaacga gggcttcgtc tttcctaaga aggggaagaa ggcgaaaccg gtgcccgtga
    19021 atctgccggt gaaagacatc gagggtgcct gccccggcat ggccgggcgg ctcaggctgc
    19081 gttacgggct gaaggtggat ctctccgatg ccgtggagac cgagtgggtc ggtgtctcgg
    19141 cagccacgga tttcattcac cacttccatt tggcgaatct gaagcccggc agcacctacc
    19201 attacgtcag tgaaacggcc ggagcggaag gcgagcctgc gcaccgcgga ttcagcggcc
    19261 ggtttcacac cgcgccgtcg ccggactcga ccacgccggt gagattttgt gtgatgacct
    19321 gccagggcta tcctgatcgc gatcatgcag acgggcatcc gatctaccct tcgatgagcg
    19381 cactcgaccc gcagttcatc tcgatgacgg gtgatctggt ttattacgac agcaatgagc
    19441 cgcgtgccac gtcgcccgcg ctggcgcggc tgcattggga gcggatgttc agcctgccgc
    19501 gtctcgtgga ctgcctgcgg aagacctcga cttattggct caaggacgac cacgacacgc
    19561 tgaccaatga ttcgtggccg ggccagaagg ccggtgagct gacttttgcc gaggggcagg
    19621 agatttttca ccaacaggca ccgctcggcg cggacagcta ccgcaccttc cgatgggggc
    19681 gcgatctgca gatctggttt acggacgggc gtgacttccg ctctcccaac cggatgcctg
    19741 acggcccaga aaaaacgatc tggggcgcga agcagaagga gtggttcaaa aagacggtcg
    19801 cggagagcga tgccacctgg aaagtcctca tcagcccgac tccgctggtg ggaccggatc
    19861 ggaagggcaa gaacgacaac catgccaaca gcggcttcgc tcatgagggc gacgagatcc
    19921 ggtcctggct gcgcgagaat gtggccgggc attttttcgt gatctgtggc gaccggcact
    19981 ggcagtatca ctccgtgcat cccgagacgg gagtgcagga gttcagcgtc ggcgcggcca
    20041 gtgactcgca cgctggaggc acgccgggct acgatgcgaa aatgcaccgc tttcatcgtg
    20101 tgaaaggcgg cttcttatgc gtcgatgtca ggcgcgaggc acgggaaagc tccatcgtct
    20161 tccagctccg agcagtggat ggcacggtgg aatacgaggc tgggttccag cgggcggttg
    20221 cctgaaaggg tgctatcccg acctggcctt ttgtcggcca ggcagccgca aaagcgatgt
    20281 ttgccgaatg aaacgatttt ggggacgatg cggtgaaatc cggcagccct gcggctgaag
    20341 gatccatccc aacccgcccc atgaaacctg gaactttttg cttttcgttc gcgctggctc
    20401 tgtcggcctc gctgccggct ctttgccagg cgcagtcgcc ctattacacg gacacgggca
    20461 gcccggtgaa tccgccctgg agtgagattt atccggcgct gaactttcag tcggcagacc
    20521 ccgggcctgt gaatggctcg gtcatcgacg tgaacaacac cctgaccgag gtggggcttt
    20581 ttggcgggcg tccgcttttg gttccctcgc agcggattca tgtgtgctgc aactctttca
    20641 ccaacgattt ccccaatttc acgcgctggt ttcagacgga tggcaacaca caggtgttcc
    20701 gtctctttgt gaatgacgaa aacacggcca atgaccgcac cgggaccgcg cgcacggagg
    20761 ccttccatac gtcatcgggc tggaactacg tcgatggcaa gaccttcgag tggagcggac
    20821 gctataccat cgcccgccgg aaacagggct actccatctt tcagtccaag aatgctgacg
    20881 atgactggtc ggtgcagctc aatatctccg gcagcggctt cttggtcatc aacaaccgcc
    20941 gccacgccga ggacgtcacc gtcaccaatc ccgatggcag caacaaggac ttcgatggac
    21001 tcggcttcga tgcgcgcatc ctggatgacg ggcgcaacta caaggtgtgg atcgatggtg
    21061 tgctgatggc ggacaattac tttgaccggc ccacttcgga cacaaacttc cgctggggca
    21121 tgtacctggg cagcagcatc ctggtgcctc ccgcggacaa ctcagtgatt ctcgtcagcg
    21181 gcgcgcaggt gaaatcctgg acgggcatcc tgacctcggc gaaaacggcc atcatcaagg
    21241 cgaacaacgg caacggccct ggtgccacca cgagctgggt cggcggcgtc ttgccgggcc
    21301 tgcacaatgt ggccacctgg gacagcaccg tcaccgccgc gaacaccatc tctgtgggca
    21361 gcgatcaagt ctgggcgggc ctgaaaatca gcaaccccgg cggcaatgtg accttcaacg
    21421 gcacctcgct gctcggcatt gatgaagcag gcgtggacat gagcaccacg acgcgaaatt
    21481 tgacgctgaa ctgccccatc gagctgcgcg tcaccgctcc gtggacagtg gcctccacct
    21541 ttaccgccac ggtcaacagc gcactgcgcg gctacggcgg cctcacgctc agcggtggcg
    21601 gcgttttgac gctcatgggt gcgaatgaat acacagggcc gacagtcatc aacgctggca
    21661 ggcttcgtct gggaaatggc ggcaccaccg gttcgctcag ccccatgagc gcaatcagcg
    21721 tgggtgccgg ggcgttgctg cacttccagc gcagcaacga tgtgcttcaa ggcaccgact
    21781 tctcatctgc cccgatcacg ggagatggcg gggtgctgaa gtgggcgcca ggaaagctga
    21841 tcctcacggc agacaacagc tacaccggcc cgaccacggt gatcgcgggc acgctgcagc
    21901 tcggcaatgg cggcacgacg ggcaaattga gcacgaacag cgcgatcagt ttctctggcg
    21961 gcaccacctt cgaagtcaat cgctccaaca acaccattca gggctccggc ttcagcgggg
    22021 aggcgatcac cggcaacgtg gcagtgaccc aatcgggaac gggaaaactg accctgaccg
    22081 caaacaacac ctacaccgga gtcaccacga tcaacgcggg cactctccaa gtcggcaatg
    22141 acggcccgac cggctccact ggcagcggca acatcatcaa caacggcacg ctgcgttttg
    22201 accgcaccgg cgcgttggaa gtgccaaacg ccatcagtgg cagcggacaa gtcatcgtag
    22261 attgcccggc agccccgccg acaccatcac cctcagcgga atcaacacct tcacaggcgc
    22321 cacttcggta aacagcggca ccttgctcgt caatgcgccc ggctcgcttc acgccgacag
    22381 cgcggtcaca gtgaacgccg ccagcctcgg cggcaacggc ctcatcggcg gcagcgtcac
    22441 catcgcctcg tccggccggc tgacgcccgg cgcggctccc ggagcgacgg gcgtccttgc
    22501 catcggcggc gacctcagtg tttcggacct ggctggcggc agtggaaagc tcttcttcga
    22561 tctgcgcgca cccaacgaca gcgacagtat caccgtggga ggcactctca gcatgggcag
    22621 cgcccttctg ggtttcgatg attttgtgtt caccggcctc ggtggtttga ccgcaggcgc
    22681 ctacaagctc atcaccgccg ccggcatcag cggcacgctc gatcccgccc atctcaccgg
    22741 caccctcggc ggcttcaacg ccacgctcgc acgcaatggc aacgacctcg aactcgtcct
    22801 cgaaacgccc gctggattca cctcctggca gaccgcaaac ggcgccagcg gagcgatcac
    22861 aggcgaccac gacaatgacg gagtgccgga tggaatcgag tacttccttg gcggtccgag
    22921 tggcaacacc accgggcaaa cacccctgcc aggcatcatg aacaacggcg gaacgctgag
    22981 catcacctgg gtcatgggac ccggctacac cgggatctac ggcaccgact tcaccatcga
    23041 aacctccgag accctcaccg gcatgtggca tacggagccc cttggagtcc gggttatcat
    23101 caatggcagc tctgtcactt acaccttccc agtaccgccc gtcacttgca cattcgtgct
    23161 gctcaaagtc aattcgccct gacctggcgt ggattgttgg ccggatcaag tccccaagtc
    23221 ctccgtggtg cgacaatcgc aaatgtttga agcgatgtgt cttatcggcc actcttgatc
    23281 gttggcgaac tcgagaccaa tggcgacccc gtaagcgcca tgccgttggt cggcgctccg
    23341 caacgtgcga aagacttcat gcccgtcatc ggcgccgccc acgacgcggc atagaagcgc
    23401 tttggctcac atctgcgcag gaaatttgtt ttacctcaac tgaagagccg aaaagatcag
    23461 acgcggcaac acctcatgcc gcatccacaa tgatctttgt cccgagcacc tcacgcacac
    23521 gcacgggggt gccgcgggcc agatagccct gctccaccat gacctcgacc ctgttgccgg
    23581 cgaagtccgc cgtgccatag gggcgcagat cgctcaccgc cacgccgcag gcccccacgc
    23641 gcaccgagcg cgcggccttg accaccggcg tgtcctccgc cgagcctcct gcggcgccgg
    23701 ccagcaccag cttctgaaaa ggggctgtct gcggcaggaa acgaaacaaa accagcacca
    23761 gcacacccgc gccgatgatg ccgagcgcga agtttcgcaa gcccgtggca taagccgcaa
    23821 ggtgaaactt ccaaggctgc ggctcaccct ccaccaccgg cagcaccggc acctcccagt
    23881 gggacatcgt gtagaccagc gccaccatga tgcagacaaa gccaagaatt ccgggaatca
    23941 tcatccccgg catcaccatc agctccacaa tgaccagcag gatgcccagc acgaacagcc
    24001 cagccatcac cgccgtctcg tgccccacca ggctgccagc cacataatgc ccgaagaaaa
    24061 acagcaggaa cgcggccacc gacaccagtc caggaatccc gaaccccgga gtctgcatct
    24121 ccaggtagcc gcccgccacg ccgatgatga tcaggatcgt ggcatacatc gtcacccaga
    24181 tggcgatttg ctcaaaggtc gtgggctccg ccgtcaccgt ctcgccattc agcccctcgg
    24241 cctttttgat gtcgtcaatg ctgtggacga tgcccttggc aaagagcggt ttcccatcca
    24301 gcagcatcac cgcctggtcc gcgtccagtg tcaggatcgt ctccttggtg tccagctcca
    24361 catcccccac cttgagttcc ttgctcatgt caatcatcgc ctcgatcacg cgcgggtcgt
    24421 gccccttgca cttcgccacc gcgcgcgcca tgcccatcgt cgctgaattc agcttcgccc
    24481 tttccgcgtc atccatctgc accccgccgc tgtaaaccgg cgtggccgcg cccgccgtgc
    24541 ccgcaggggc catgtaaatg ccgtccgtgg ccaccgcgat catcgccccg gcggagaggg
    24601 cgcgtttgtt gacgaaagcg aaactgcgcg gcttgagttt ttgcagatcg ttcatcatca
    24661 gatccaccgt gtcccagagc aggccgcctg gagtgtcaat gtcgaacacc accgcctccg
    24721 ccccctccgc cgtgcagcgc tccagcgtgc ggctcatgaa ctcaaagcgc gcccgggaaa
    24781 tgaggtcgtc cttccccacc gggatcacca ccaccctgcc ggagtagctc tccgcccgct
    24841 caccaagggc cggcagtacc gcgccgagga tcatgacaag tatcgcaggc aggaaagaaa
    24901 ggtgtcggat cattggcgga cgttgcttgt ttttaacaac tacgcatggc gcgcgggatg
    24961 gcaacgtgtc gttgcagctc ctccagccat gcctcccgcg ccgactcctc gtccgtccgt
    25021 gtggcgcgga tcatgcggct tgcgcggaac ctggacacgg gttgaatgcg cgccgcgcgc
    25081 atctggaaaa aatgacgcgc aaatctcccg acgacatggc cttcaacacc gcgatttttt
    25141 atgatgtgga gaacctgctc aaaggctact ccttctcgtc ccacctgctc aacaacctgt
    25201 ccttgaggga gattctggaa tcggtggaca agaccggtct ggtcaaaggc atcgccgtcc
    25261 agcgggccta tgcgaactgg agcgacgcgc gcctggcggt gatgcgcggg gagatcagcg
    25321 agctcgggat tgatccggtg caggtcttcg gcttctccaa ggaccagaaa aagaacgcgg
    25381 cggacatcca gctcgtcgtg gacgcggtgg acctggcgca tgtgcgtccg gccatcgagg
    25441 tgtttgtgat cgtgtcaggc gacggcggat tcgcggcgct ggcgaagaag ctgcacgaat
    25501 atggcaagac ggtgatcggc tgcgcctacc ccacggcggc aaaccgggtt ctgcgctcgg
    25561 tatgcgacgc ctttgtgccc atcccggacc ctgaggatga gcgcgaggtg ataccactgg
    25621 cgcgtccgca gccgccgcca gccgcgaacg gcaagccgcc gaagccaaaa gcgccgcagg
    25681 ctgctccgcc tcctccagct tccctggcgg cgccgtccgc gcagacgacg ccggccgctc
    25741 cttccgtgca tccggtggtg aggaggatgg cggcacaatt cacccggcgc gagccaaagg
    25801 accgccagga catgatgcgg atgacgcgcg aggtgatcga gtggctgggc agggagcagg
    25861 ccggcgctct gggacgcggc ctgcacctgg ccacggtggg cgaggcgctg cgccaataca
    25921 tcgcggactt cgcgccggaa aaagcgggct tcgccaagct ggcggacttt ttgcaatggg
    25981 tgtgcgccgg cacgcaatac caagtggcgc gcctgccggg cggggtgcct gcggtggctg
    26041 agcgcgccca accaccggcg gacgcggagt tgctgccgga cctggacgaa acctggctgc
    26101 acagccccga gcattaccgc tcgtgcctgc gcgcgggcct gcccatctac agactgccgg
    26161 agatggaggc tttgcggctt gcgggcgctt tcctcctcac agaaaagccg gcgccgatgg
    26221 agctgggtcc gctcatcgag atggtgtcag cgcgccacgc ggacgagctt tccctggagt
    26281 cggtgaagcg ggcgctcctc tgctttcatt cggcgggatt tttcctgcgc gaccccgaag
    26341 gcgcgccgct ggcggaacag cgtctgtcat tgaagcctga actccaggag accggcgacc
    26401 tcgtcgaacc cgtgagaaag gcggtgctgt ccaagctggc ctccatgctg ggcagggtgg
    26461 atgacgcggt ttttgaagat atgctcgccc ggccttcctg agcgaacggc cgcggtcatg
    26521 ccggcaggag agccgtcttc ccccatttca cagcccggca acccgcttgc ttcaaatgcc
    26581 acagtgtcat tgacaaggaa tgtggcggtc ggggcttgat tggaaccagc gcagccgctt
    26641 catccttcaa gcctgcgttt tttgcggaat ggagttcatg aaagtggaga gaagctgcga
    26701 agactttctc attttgaaat gcgacgctag attttcccga tgtccgccag caaagtcctg
    26761 ccctatgaag acagcgccag cgcgcgcgag gagttgatcc cgtttttgac cggggccttc
    26821 ccgggcgggc cggacgctgc gcgctggcgg ctgcggctgg cgcattggtg ggatgagaac
    26881 cctttctgcg agccggggtc gccgcgcggc tgggtgctgc ggcatgaggg gcggctggcg
    26941 ggtttcctgg gcctgatccc tgcgcggtac gctgcacagg gcgcgcctgt gtccgccttg
    27001 ctgggcacga gctggtacat cgtggagggc caccgcaacg ccgccctgcc gatggccatg
    27061 caataccagc gccaggcagg cgcacacctg ctggtggaga ccacgcccag cccggaggtg
    27121 cgcgccatgc tggagcgcgc gggttggacg ggggagacgc gcgcgcgccg cctgctgctg
    27181 ccctgcggag cggtgggcag cgcgctagtg gcgctgcgcg gcaaggcctg gccgtccctg
    27241 caacccggcc ggcgcgtggt aacggacacg gcggaggtgc tggcggtggc gcgcgcgtgg
    27301 cagaggcctg accggctgga gaaatgggtg tgcccggcgt ccctgcgctg gtatgcggcc
    27361 tcgaccatgc gcaggcattt gttcgccggg gtggtggacg gggcgggctg cctgtcgagc
    27421 ttcgcgtggc tgactcctca tcactccggc caggcgcgcc tggcggtgct ggagggcttc
    27481 accaccgagg cggatgatac ggaatggcgc gcgctgatgg gcgcggtggc gcgcggggag
    27541 gcggcatggc ccggcgcgcg cgcgtgggcg ctcacctggc tggccttcga cacggacgaa
    27601 ctggcggccg gggcgccctt tctgcgcgcc ggttgggtgg atgtctgcca ttaccacaag
    27661 aagccgcccg ccctggcagc gcccaagcac agcgtgatgg cggaggggga cttcggtttg
    27721 tgaggcaggg cggggagtgg ctgcggcttt gctttctccc tcccgctggg atcagcctgc
    27781 ggcttcctcg atgacgcgca gcagattggc ggcggtggca aaggtctcaa cggtgatttt
    27841 ttcatccggg acgggcgcgc caaactcggc ctcgatgtcc atgagcaggt ctatggactc
    27901 cagcgagtcg aggcccagcc tgccgaggtc cgcgtccagg ggtatctccg ccccggcggc
    27961 cagcaggcgc aggcgcgggc ggaggatctt gagaagacgt acggtggtgt ctgcggctgc
    28021 ggtcatggat caggaaaggg cggctctatt taaccgctca tgcccggtgc gccagcgcca
    28081 gacctccaaa acttcacccc caagatgggc gatctccagc ggcgcctggt ggctgaggaa
    28141 accgaggagg ctggcggtga ggccgtaggc gcccacgttc gggatggcga gcaggtcgcc
    28201 cggtcgcaca tccggcaggg ggatgccgcg cgccaggctg tccagcggag tgcagagcgg
    28261 tccgacaatg tcggcgggcg gggcggcggc gttttccggc gcgccggaga ggttcagaaa
    28321 cgtcacagca ccgcgcggga tgcgccccag cccggccatg ccgcccaggt gatggatgcc
    28381 ggtgtcgagg atgacgaagc gccgcgcgcg ggagtgcttc acctccagca cgcgcgccac
    28441 cagcgtcccc gccgccgcgc tcaggtagcg cccggcctcg aaccacagct ccgccgtgcc
    28501 gtgcaaggga ctctccgccc atgcctcctc caggccctcg cgcaggccgg tgaggcaggg
    28561 cgggggcgcg tcattcgcat acggccaggg gaagccgccg ccggcgttca ccaccgcgca
    28621 gacgaaaccc agtgcccgct ccagcctctc cgccactgcc agcgcgcggc gcgtgttctc
    28681 caccagcgcc tccacgcccg ccacctgggt gccgaagtaa acatgcacgc cgcggatgcg
    28741 cgcgccgggg aactttgcca gcgcccccgt ccccgcagcc agcagcgcct cctcgtcaaa
    28801 accgaactgg ctctcgacgc cggtcatcgc cagccgggcg ctgggcgcct ccgccgggtt
    28861 cacccgcagc agcacctgcg cgaccgcatc cacacgcgcc gccgccgccg acacgcgacc
    28921 gaggtccgtc cacgactcgc aggaaaacca gcgcactccc gccgccaaag cctccatgat
    28981 tacggcctcc gtcttgcccg gcccgccaaa gagggcctct cgcaggtcat gccccgccag
    29041 ccgcgccgcc tccatctccc cctcggaggt gatctccgcc accgcgccac ccagccgcac
    29101 ctgtcgcgcc actgcaggca gcgggttagc cttaaaggag tggaaaagcc gcgccccatc
    29161 aggcagctcc gcgcgcaacg cggccgtgct ggctgcgacg atgtccagat catacgcata
    29221 aaggggcgtg ccgtgcgcgg cggccagggc tttcaagaaa tcggacatca ggaaacgtaa
    29281 gagcgggatg cgcggttcgg aaagcgaaag tctgcccagg gtagcggcgc ggaaccccat
    29341 gccgcttatt ttcggaaaac cgcataacaa acgggatgcc acccggatag atgggatgaa
    29401 gcagcatccc aagacgccaa cccgccatga aaacgatcct actcctctcc gccttgtccg
    29461 ccgccgccgc gctggcccag cccttcggca tttgtttcgg ccccttcaca ggcaatcaag
    29521 accccgcgac cggagtcgtg ctgcctgagg cccagcttcg cggcaggctg accccgctta
    29581 gaggcaagtt cagctgggtg cggtcctacg gctccacgca cggcctgcag catgttggca
    29641 ggctcgcgag aggcatgggc ttcaaaacag caattggcgc gtggatcggc ccggaggaca
    29701 ccccggaggg aatcgccgcc aaccaggccg agatcgccgc actcatcgcc gccgccaacg
    29761 ccgggcatgt ggacatcgcc atcgtgggca acgaagtgct cctgcgcggc gatctcagcg
    29821 aggagcggct gctggagatc atcgcggagg tgcgtgccgc gataccgtgg tgggttcccg
    29881 tcaccaccgc ggagacgcac gacaacctgc tggctcattc cgctgtcatg gcagcctgcg
    29941 atgtcatctg cgcgcacttt catccctact ggcagggcat ccgcatagac gaggccgtct
    30001 cctggctcga tgacgagtac caggccctgc gcctggcagc gggcggcaag tggtgcgtga
    30061 tcggcgaagc gggctggccc agcgagggcg accccgaaca actcgccgag cctggcttgg
    30121 aaaacgccgc gtatttctgg agcgcgtttc aaagctgggc cgtccggcgc aacatcccca
    30181 acttctattt ctccgcgttt gacgaggcct tcaaggaagc gctggagccg aaccgggtgg
    30241 gtaagaattg gggcatccac acggccaagg gcgttttaaa gcccggcttc aagaatgtct
    30301 tcgacgacaa actgctgccg gaggcagccg tgctgcgagc cgccgtcgaa cccccggctc
    30361 tgcctccccc gccgtccgtc gccttgaccg cgatccccgc ccacggagtc aaggcgcaga
    30421 aaacactttg cggagttgtc tccgacttgg gcaaaactgc tgcctatcgc cggctcgcct
    30481 gctacatcga agtggacggc ctttattgga caaagccgtt ttttaacaag ccgctctttc
    30541 ccgtcctgcc agacggcgcc ttcacgacga agatcgtcac tggcggagtg gaccagaaag
    30601 ccactgagat cgccctgttc ctggtgcctt ccgactataa ggctcctctc gccgccggtg
    30661 tgccattcat tcctggcagc attgaacagg acgccctggc atccgccttt gtctcgagac
    30721 ccgaaggctc gcctgcgggc ggcgatggcg agcccggctt cacggcctcg gcctcagtcg
    30781 tgcgcaaact gatagccggc tcagcttccg agagcgcctc cctgaaaggc attgtggtcc
    30841 tgcctgccga ctttgaggct gcgggcgttg tcgtcggcat ctcgctctgc gggctggagt
    30901 ggtccttcaa tccgctgggc aagtccggct ccgcagtggc ttctgacgcg tctgccgcca
    30961 aggccgtcgg ctcgctttca ttgaagcgtg acgccaaata cggcttctgg cgctggagcg
    31021 ccgccctcaa gcgctccgcc agcgccagcc tggatgccta tggcatgatc aacgccacca
    31081 tctcgaccgg cagccccgtt ttcctgccgc tcgccctcga cctggacggg caggcgtttg
    31141 aaacggtctg ccgactccac tacaccgccg cgcaaaatgg cacgggcacg gcgaaataaa
    31201 tttttttatt aacagtcacc gctcagcgcg catatcccgt tatgcagagt caccgcgcat
    31261 ccatccatgt cctggcctcc gcgatagtcg cggaggccct ttcccgtaca ttcaacccgc
    31321 ccggcaagac ttgcgcgcat ccccaacctc aacccgccgc tgccatgccc tgcccgtatt
    31381 tacccaccct caaggttgct ctaaacacac tgctatccct ttggccggat tgccccctca
    31441 agcatgccag aggcgactcg ctggctgaat acctcgcttc gatcgaaaag atcattcgcg
    31501 ggcttggcga agaggaatcc gctgccagga aggcgggtga agcagcccag gatgatgacg
    31561 tggacgggga catgacccca gtacactttc cgcgtttgac tcatggggtt gccaactggc
    31621 tggatgcctt tgtttttgct gctggtcatg ccccgaaaga tgcctgcaac gctctgaccg
    31681 gggcattcga tgacagcgca ggtgtcacca atctcctggt gatttacgcc acaatgtgcc
    31741 ggctacctga cgaaaaagca gaaactgacg catggtggct gctgcttcag cgaatgcacg
    31801 tagtcgccaa gaggcgagca ataggcgctc tgaactctga gagcatcgcg gaagaagcaa
    31861 ccagtgaagt ggtaaaaaag atcttcgaag cgaaaaacaa tgccgcgtgg cctccgaggt
    31921 gcgcccctgc ggcgtgggtc gtgaactcgc tcaacaaggc agttgtgagc ttgctgaata
    31981 aggcagccaa tgagtcaacc cgactttcat cgcttgatga ccagcatgac gccatcgccg
    32041 aaaaaatggg gggcgacagg tttcctcgcg gcatcagtga ccgcacctgg cttcttcttg
    32101 attccctcga gaaggttaaa gacatggaag atgccggcaa taaatgtttg accccgcgcg
    32161 agattgaaca tctggagtgg cgcgtcctct atttggtgac aaagaagacc gcagcagacc
    32221 gcgcagtcgc tgcgctagcg agagacatcg tgatccggtc actgactgac gctggctgga
    32281 cttttcgcac tgcgggtttg agttgggaca aactgccagt cacccgggaa gaagtggcga
    32341 aatgcctcgg tttcactata gaaacagagg ccgtttattg gcaccatttg aggagaaaaa
    32401 tcaggcgcat catcaaccca tagtctgagt cacaaaatcc atcaaaaacc cctgactaca
    32461 caaatccaaa tgaataccga aaacgacctt ccagaggatg atttcatcga aaccgcattt
    32521 caggagctcg ccgatgcagc cgccttcgaa gcggccgcgc ttcaagaagc ccgggccatc
    32581 tacactcccg agcgggtgcg ggatttgctg gcccaggcaa gactgactgc agtccagcaa
    32641 ccccggctgg accagacaag acagactgcg gagagcagag ccgccaacat gagtgtgcta
    32701 gccttggcag cctcagccgg cgagcccttg acagaattga aaaaaagtct cgaaactgac
    32761 ggcttctgat gaagccgccc ctgtttcaac gctggtgcaa ccgttttctg ccgggctttg
    32821 gctcgtggcg gaaagccttc aggagccacc tctccgcgtc ttctctgacc actctctcgg
    32881 ctgacgaatg gcgcgacttg ctcgtcaagg agtgtgagac agcacagaag ggatcgcccg
    32941 gtgatccttg ctctgcccaa atccaaagaa ccattcaaaa cgataaatgg cgctgcctgc
    33001 tgcgcgccta ccctgaggcg gccatttttc ttcatgacaa aggatacgag cacagtgatt
    33061 cgaaacacaa ggctgagctg gtggcggagt gcacgggtct acttgaaaca accggcctaa
    33121 ccagtgcgga tgcgtccgcc gttctggtgg agtttctggg cacgcggcct gactgccagg
    33181 ttcgcatgca ggtggtactg gttcaggaat ctccaaccag ggctgtgctg gctgaactgg
    33241 ttttgactct ggtggatgat gctgtagcag ggaactcatc gtgctcgttc gtcccctcgc
    33301 cggccatgcg cctgtggctt ggatgggatg aggaacttca gactctggcg gatgacttca
    33361 agaagagcct ggttgaagca tacatccagg cttgcgagct agccgagtgg ccaaaatcct
    33421 gtcacatcat ctggagcctc tgcttcagcc gtgaacaccc cttcagccat gaacaccccg
    33481 ccgcccccga tctgagcggt aagtcgctgc aactccccgc ggccattgga ctttccctgc
    33541 tcgccgcccg caactccagc gtgagatgga aggctggtct aaatcgagat gcgatagccc
    33601 gcatggaacc tcgttcattg ctggccacgg gctgctttgg ggcatccacc gacgacctta
    33661 aagcaaagct ggccaaattt ttcgagttca catcgaatga ccgcgagtcg tttcatgccg
    33721 tcctccttcc tgaagacgat aataatctcc tgcatttggg tgaatccccc ggcaaatatg
    33781 agcggacatc ccatccgcgg acctggcttt gccggcaaac taaaaccatt cccatcgtgg
    33841 ccaggccggg cctgagcgag cttctccagg ccgtggtggt tgaaaaaatg attcacgtgt
    33901 cgagggggcc tggattccaa ggccggctgg taacgcctcc tctcgcttca tcatccccgg
    33961 cgaccgcgcc tgaccccgtg gccgattgga tggaggctga atcccctcgt gtcctcgtca
    34021 tagaaaacga tgacgctgac caggcggccg acgcgcttgc ccgcgcagcc tccaaaaccg
    34081 gttggacagt catgggctac tggcctgaca gcaataccgc cgacccgctg gtggtggtgc
    34141 agtccctggt ggcccaggcg cggcgcgcca cccagaattc tgacgctgta ccatccggag
    34201 gtgcgtttca ttccgatccc gttgaaaggg aactgcaaat gctggctcac tgcctggaca
    34261 gtctgcctga gggttccaaa gcgttgatga ttctagccgg ggtggatttc atctccgacc
    34321 accatggccg ctcgagtctg cctgcgcggc tggctggcat tgtcaggcgg catttcaata
    34381 ccaattccga actgcgcctg atcctacagt caggctcgta tgccggagtt cccagaactg
    34441 acgacgttaa gtttctggaa cttgccaaag gaaaagccgg aaaaggcaat gcctggaatg
    34501 ccgctggact ttgcgaactc ttgcttgcac gcgacgatca cgatgatccg ctggggtggc
    34561 tggtctgcgc cagaagattg tttcgccggg acaagacctg gagaaccctg ctgggcgaat
    34621 cagaggctga atcattgaag aacgccattc agaatgcgtc cgcagttttg aacgtgcccg
    34681 aggaggacct gtggcgaatg tccatgatca caaggcggca gatcatggaa caattatgcg
    34741 aaaccgacac aggcaaagag cgggtgaaag aatggcactg ccggtggggt cgccggcttt
    34801 tcatgttgct tcacaacgag cctagccccc aggatgctgc tggtgacatg cccgccgcga
    34861 gagaagccag acagtacaga ctcgaccatg ttctgggtca cttgcgcctg gggcgcctct
    34921 gggacaactt tcacgaagcc ctgcagaacc ccgggtttat tctcgaacac cttgcccagc
    34981 ccaggggagt gaggcgtttg gccaagagtc tcgaaaaggc actgtctgga aaaacctgcc
    35041 tgcccgttga ccatcctgcg cgcccgcgcg tgtctgagct ggcttttgtc attcatccgc
    35101 tggcggatca cgttttgaaa attgacgaag cactggcccg gcagtgcctg cacaactcca
    35161 tcgtggagca gaaggagacg ggtctccgac actggctgaa gctgtttctg gctgcgacgg
    35221 tcagactgga acaagccgag caggcctgtc tggaccaagc cgggcaggcc tggctgcgcc
    35281 tgctgccgac tgctagtggc acaagcacgc tggggttgct gcccagccat accctgcgca
    35341 aacccgcgcc catggtcgcc gcagccttct gcccccagct cacggcccaa ggcaggccgc
    35401 aggtgctgac attttcaagt gacaccgttc aactttggaa actaggcgaa acggaacgtt
    35461 cctttgaagc gaacactcac tgggcggggg caagctcgat ccggcagact ccggttcgga
    35521 tgcttgttcc gggcttcatc aatggccgtt acgccattct cgtctcagcc tatggcgttt
    35581 cgattttcga cacaaagaat ggctcatgcc agttgaacat ggtgaagctt tcgttcaggt
    35641 cgctcacatc cgtgcttcac ggcagacggc tctacctgag tgtggccagc acgccaccgc
    35701 aactgatggt ttatgaggtc agagacacag gagacgtgag caagcctttc agttttgctt
    35761 tcgtgagtga gtggaaattg tccgaggaaa aaatgctcgg cggtgatcac gcgaaaaaaa
    35821 atgccggtgg agtgactgac gaagatgctg acagagacat ctaccgtagc ttggccgtga
    35881 cgctggatgg cagtcttttg gctgtggcaa agatggatgg agtggttgat gtctacaaca
    35941 cccctgatct cagcctgacg caccaggatg gagtggttga tgactacagc gtccctgatt
    36001 tcagcctgaa gcaccagcta cccgccaggg accgccgagt ggactcaatc accttcgacc
    36061 cggatggccg ctttttggtc gtgggcggag gcttgaagtc ggggcaggtg gaaatatggg
    36121 atctgaacga gtgccgggcg acgattgcgc gggtgcatgt gaactcgatt tggtcactgg
    36181 gcttcgtaag gcatgaaaac gaggattttc tcatctcagg cggctatgat cgctttgtca
    36241 aagtcttgtc atggccggcg cttctaaagc ttcatcaaga gcacaagccc aacccggcgc
    36301 ggcaggaaat caaggatggc ttgtttaatt ccatcaatag gcactaccat cttcactatc
    36361 tggatttacc ttcgggtgtg gtccgctcgc agcgcaaaac ttcggacatt ggcatgtctt
    36421 tggacacagt cggcggctgg ctagtcgcgg gggcggcggg gcaccccttc gcggtttacg
    36481 agatgggcga gcttttgcgt gatccaggat cgggtgtcac aacggcgccg gttcacacgg
    36541 cagttgtcgt tggcgcgccg gttcatgcgc ccatatttgt ctgggctggt gatgacggtt
    36601 gcatctactg tcgcgctggt cttgatggag ccattcaagt tcatcaagtg ggccttgctg
    36661 aaatccacag gattgacgtt tcacaaaact ggattgcagc ggcaacagta aacggagtgg
    36721 ccgtcattga ccgccagacc gccgctccgt cagtcatttg gaatccaacc actggtatcc
    36781 ggtccgacgc ggtgcgcatg ttcaaagccg gcgggcggga cctgctcgtc tgcggtgact
    36841 gcaagggctc tgtgagtctg catgatctgt ccgcgcgtaa cagactctgg agcaatctag
    36901 aaagcgatct caagagcaag agcaagatcc gcgaacttgc cgtgcgcgtt tcagacgacg
    36961 ggagcaagat ctggatcgcc tcctgcgggg atgattcgag tgtgcggatt tcggtgttgc
    37021 gattctcatt ggactccgca actgagccgg tggaggaagt cgcaaccatt gaactctcag
    37081 acccgggttt gaagggggcc aaagctcacc gtggccgggt taagagcctg ctctggtgtg
    37141 gtaatagcct ggtgaccggc gggcatgaat tcatcaaggt ctggcggctg gaaaatccca
    37201 agggcgagtc gcctgttttt tttccgccgc tgaagctcga aggccatgtg ggtgaaatct
    37261 ggagtctgga ttatcagccg gagcttgacc ttctcgcatc aggctcgcag gatcagacgg
    37321 tcagggtgtg gcggctgaag accgcgaggc tgctggcgag cgagccgcgc aaggcgcctg
    37381 tcgtgtgtgt ggcctggcaa aaatgcccgg aggcgggctg cggccccatt ctccacgtcg
    37441 ttgacaccgg cgtctccgcc gacccccaat gcagctctta tgagttaaga gggctgcgtt
    37501 aagcgcgatc cacagaggca ctccatccat tccgcatgtc accttgtagc gcgtgcacga
    37561 acgggcaatc tatctgcaat cgcggaccga cagcgcaggc caggcaggtt ccctccactg
    37621 ctatgcgggc atccgcgccg aggtccgcgg cgccgataaa gtccacccga tggggggcag
    37681 acacggtgag cttctgacct ggcgtgtcga gactcacttg aagcccgcct tgcccgcgca
    37741 ctggctggaa ctcccagccg caggactgaa actcacttcg caaaaaggaa atggtctcag
    37801 atctcccgtc acacaccagc agcacggatt cggcgtctcc cgcccctgga tgcttccagc
    37861 ccggcagcct gtccttgccc gcgcgagaaa ccagggaaga catgcgactt gccacagagg
    37921 gggaaagata gcttttcgac cacgcttgaa actcgcggtt gtagtccaga aagccctggc
    37981 cgcaggccct ccagtcctgt ttttccaggt tccgcgtcat ttcaccggca agcgcggaga
    38041 gagaacgcac gcgacagcct gcaacgggat cggaaaagca gttggaaaga gcggcggaga
    38101 cgctgtgcat tttcccgttt ggattccatg ccagaaagaa gtgacgggag aggaagtcgc
    38161 tgtccgcatc cacaacccgc gtccgcattc cgccgaatgc caggtccacc gaggcggaga
    38221 caaccccgcc gtgaacgatg ggataaacgt cattgccggc aatggcggca ccggcagccc
    38281 gcctttcgtc gtcgagcgcg gtgcgggcaa tgacgctgcg gaaatgctca ggcgggaggc
    38341 ggcccgcaag cagttcaaga agcagcggcc gcattccaac ggagacagcg gtccgcaaca
    38401 cgctctccac gctgctgctg gagaatccaa gaccgcggag cggactggag gggatggcaa
    38461 cagaaaggct cgaagacatg gcggtctcaa gtgagggttt gttcattgaa ccagatcgcg
    38521 cagtgcctga tcctgcgcgg aggccagcca ttcccaaagc cgctggtggc aggcctcgcg
    38581 cgcgctggtc tcgtcatgga aaacccactc tcccagcgct tcgaggcaag ccacaggaaa
    38641 agacgcatga acccgcgtgt cgcgaggaac gacataaaga ttggcgccag aggcgatgat
    38701 gctggcggcc gcatgggcat ggccgggcac atcaagcagg tccagccaat gttctcgcaa
    38761 cgcagccaga atcgagtcag gggtcatggc ccggaaaaca aagaaagcca cgggataagc
    38821 agacggcacg ttgaaatagc cggcatcgtg ccgggcagca aactctaaag gatagacccg
    38881 ccgccagcgg ggcagagcct ggtaatgcag atggtggggc aaagaagcgc cggcaccggc
    38941 attgtgcagg atgaggaaat ccggcaactc ggcggccaat tccatcatgt cagccagacg
    39001 cgcccccagt gtgagggcga cttcatacac gcaagccgct gagatgcctt gaggcacatg
    39061 accgtcttcc gcgcgaacga ccgtgaagag gcgctcggag aaggggaacg gattgatcaa
    39121 aaatcgaaac ctcccgtgcg caatcccgac ttggcggccc tggctcaaca aggagtagtc
    39181 acacagcgga cagcggccgt tggcggaagt cttcggtcgg cgaacacgat caggagcgac
    39241 ggaggcgaag aacgagggtt tccccggcgc gcaaaactgc agccgcttag aggaaggcga
    39301 ggcgacatcc aacaacctga tctgggcatg ccaaaaaaaa tcgagagcgg cgcacaaaga
    39361 ctggttggaa cggatggcac taaagtcagc ctgggtcttt tcttggatga agccggacaa
    39421 tgcgtccacg agactgtccg cggcggaggc agattgtggc gggaaggacg ttgcggtcat
    39481 gcccttgtat gccggcgaag gaacgaatgt tatgtttttc gcgcccggcg aggctcaaaa
    39541 atcaaaaagg gatgtcgtca tcggaggtgt cgtgcttgaa gcctcgccag atttcaaaac
    39601 tcgcatccac gaacttccat gcggttgccg gctgctcagc cgtgccgagc cagcacgagt
    39661 tcaggcgctc catgcaggcg gagcggactg agtcaaacaa cgcgccctcg actgggcggc
    39721 ataattcacg gatttcaggc atgtgtgaca acacgcccgg caggcgcgcc gccaactcac
    39781 gcatctccag actgccgcca gcctgggctg gtccgcagcc gacaccgagg aagcgcgccc
    39841 tgtccagcat gtccttcatc caatcctgcc agtgaagata ggcatgagtc atgacatccc
    39901 cgtcctcgct ttcaaagctg tagggggcgc ggacgtgttt gcagtaatca tgcggtgcgg
    39961 cggcgacacc agcgaacagg atgccaagca ggccctccat caaagcagag aattctctca
    40021 ctacaggctt gaagaaaaca aactcggcag gcatccccgg ccaggccagg atgtgccgca
    40081 ggaagcccgt gccgcgaatg aatccgggac gccgccagcg tgtcagttcg cacacccttc
    40141 cgctcaacga tttcacggac tcctgccaga gcagcgcaaa ttccttcgaa gcctgcagcc
    40201 cgtccttcct cccagtcagg acatcctccc tcatcccacg catcagatca tggaggcgct
    40261 gattgtggat tcgcgcctca atttcgagag cggattggat ttcggggttc agcggcatga
    40321 cggccggcgc ctcctgttga tccggcccat gctcttgcgc gccctgcgcg tgttgcaagt
    40381 catgaggcag agggtgaaaa tcattcctgc gcatgcctgg agggtggcaa gcaccatccc
    40441 tccagcatca tgccaccggg ccagatattc ccagtgtctg gtgacgacgg cggcacaggc
    40501 gagcgcgagt ccgaaaacag aggccgccca gaccgcgcgc cgcaatgaga ccggtgcaaa
    40561 aagcatcaca ctcatgccac aagcaaacgc gagcgtgccg gaacccgctt caaccggcag
    40621 caatgccaaa ctggaaagct cgatgagcca ggtgacggcg caactgaatg caagcgctgc
    40681 cgcggaggtg agacagcgag cctcccatgt ggagacacga accagggcaa tgatggagac
    40741 gactcccggc agagctgcag gaatgaggag gccggctggg actggatgca ggaaaaccgc
    40801 cgcgagcagc accgcggagg gcagccagac agccaggcac ccgccacgca gaggcggggg
    40861 gcgtttgatg cgcgcgttca cgggcatggc aatgacgggc acggattcac aaaccccaga
    40921 agcacgggtc atgtctggcc gcgggcagct tggaatggag ggcgtcgggc agcggctctc
    40981 ccgacaccca ctgcgcccag cagtcctcca aaaccagcag gccagggtca ctggcgtggc
    41041 aggagggaac gtccccggac agcaatccat tgacagcatc gaagaccgag tccgcctcat
    41101 tgtccatgac gcccctgatt ctggcttcaa gcacacgaac ccgcaaaagt gccgtccggg
    41161 cctcgaatgc ggcaagcatc accctggaac gcttgagggc atcaagcgcc agcgagcgct
    41221 ccccgcgcat gtgcgcgagc cgagccagag tgggcaggcc aaactctgcc cagtcgccga
    41281 aaaagcccct cgactccagc acactcgcag cctccaccac cttcgagaaa ccaggctcca
    41341 gcgcgccttt catggcgagg catctggcaa gatcgcagcg cgcgcgcgcg ccgatcctgg
    41401 tacaggcaag tgatccggcc gccatgcgca atttttgctc ggccgcatca agctcgccca
    41461 tgcgcaggag cgccgccccg gcaccgctgg caaaaaccgc ggagcgccga ctgtccgcat
    41521 tgccgccgac tcgaatccag aggggagcct cgggacgcgg cccgaacccg agaaaccggg
    41581 agaccagcag atggtctatg gacaggagga gttcatccgt catatgcagg ctgtgctcgt
    41641 ccagagtttc aatcgcctcg ctggcgcggc ccagacgggc gagaatccac acacgttgag
    41701 agcgcgcatg gtcattgtcc gggtattggc tgtcgagttc cgccagtgct tccgtgtagc
    41761 gcccaagctc gtagagccgc atcggcggat agctttggga tgattcaaga agacttttgg
    41821 gtgagtcctt gaagacaggc cgccaatgcg gcgtgagaca ttgcgtctgg ccaagatcca
    41881 gcgcggcgtt tcgatgcgcg tcaaaaacgg aggtccacga ggcatggctg ccatcgcccc
    41941 ggcgcagggg gccttgaatg atgatctccg agatggcatc acccctgagt ttcgcgcgag
    42001 tgtcctgcgg cggagtgatc ctggctctgt gaacctccgt ttcatcaagc agaagctggc
    42061 cgtcaggcag gcagccagcg gcggccatct ggtcaaaaac tcccgtttca tgcctgccat
    42121 acgcgccgta tttcatgtcg agcgcgagca gggcgggttc gttgggaagg gtgaaagccc
    42181 cctgcgtctc gaagatcatt ctcttgagcg gccactgtag gtacatggcc cgctcccagc
    42241 cgtggcgcag gtcttgcagg gcgcttttcc aatcctgaag aaaggcgccc gcccaggggg
    42301 gcagttgatc aaggtgacgc tcgcaggcat ccgcgtacgc ctcctgaatg tcgagtgcgg
    42361 acatttgcgc gccgtttctc aaattgatca gcgccctcag ggaaggatcc cagttcacga
    42421 cgcgcagcgc gtcagcggga gagtcgagcg cgaatttcga gaaggccgcg ccgcgcgtca
    42481 gctccgccac gcgcgccacg agcatcgtgg agccgcggtc cagcatgtgg gcgcggtcgg
    42541 aaagcaggca ggcgaagccg atgagatgga ggcggtgatg atgcaggcta tgccggtcac
    42601 cttttgtgca aatgagcggc ctctcgcgcg cggactctga attgcctccg gtgacgtggc
    42661 cgccgaaatg aaacatgcga ggagagagca gatatgtggg ttcgccgggc ttcaggggat
    42721 cactcctcgg aacaaagccg ccttcgccac cgagaagcat cttcgtcacc agatgcggca
    42781 ccagcccgtt gtgctcgaag tcgtccgggt gggccgagga ttgaatgttg agatgagtcc
    42841 cccaggaagc ggcggcggcc ggatcgcagt tggtgcgcag aaagctggcg cgtatcccgt
    42901 cgccgccggg catggattcg agcgtcctgt cgagaatgtc gagcatgagc ttttccatgc
    42961 cgcgcacctg gaggcgcaga tcgcgcgggt tcgtgcattc agcggaggca aattcgagat
    43021 gctgtccgcc ccgcgcgcgc gcgccgccgc gcgcggcgtc ccggtagatg ctgccgccgt
    43081 tggccacaaa gactcccccg tgccagcctt cattcggaat gccaaaccgg cgggcattct
    43141 ccagaaacgc gggataaatg tcgtcctcgc tgacacgcct gccgcccagg aacgcggcga
    43201 agccaagctc ctgctcctcg ccgcaaatga tggccgggtc tctggcggtt ttcatcactg
    43261 ggcgcttttt tgtttgttgg cctgcagata cgcgcccgct tccaggccct ggagcgcttt
    43321 tttgaggatc tcctggctgg ccttggcgag ctgggcgttt tcctgcgcgg cagcggcgag
    43381 gatgccttcg tccgcgtcgt cgtcatcgca gggttgcggg gcgcgggtgg tctgttctgt
    43441 gcgtgtctgt tgtgtctggc tcatggtggt ggtggggttc gaatgacgcg ccggcattca
    43501 ccgccgcgca aagtttggaa tagtcgccgg cgcgtccggc gggcgtgtcc agtccgggcg
    43561 cccagggcgg gggcatggcc agccggacgg gacggccgcc ggggtcgagg tcgaagatca
    43621 cctcgcccca gtccaggctg cgcagatgat cggccgggat gactcgaatg aggcgggcgc
    43681 gcagggcggc gcgcgtgttt ttcggaggct gccggattgc ggcatcgaga tcggcttcgg
    43741 tgaaaaacgt catgcccttg tcagcgcgct ccatttgcaa atacaacccc tcctcgtcca
    43801 gggaagcata caaatggtcc agcgccttga tctccggcga ggaccagtcc agcgcgggat
    43861 cgcaggccat ggcggacttc agcaggccga gtttgctcca gccgtccagg tgcgcggcca
    43921 gcccggagtg atcccgcgcc cgcagcgctt ccgaaagcca gcgggcatgc atgaggatgc
    43981 tgccggcgcc gggcaccgtg tcctcaaagc cttccttcca tcccgcaagc agatccagaa
    44041 atctctcgaa ccattcgatg atgcccgtct cgctgcccga ctgcgcgggc acggcggctt
    44101 tcagggccac gtcccggctg aagcgctgaa aggcatgcac gggatcgcgc agaatcagcg
    44161 cctccggcag gggcgcgcac gactcgataa gtgcgaagag caactgcagc agaccggtct
    44221 tcatcaaatg cgcgctctgg gaaagtgtga agtcaaaggg gatgatgtgc atgcgctcca
    44281 caccgtgccg cgccgccgcg tgcggctcct gccgcgtgtt gatgaggccg cggttcggcc
    44341 actgggtgga gaactcccgt gtcacgttga agaagtcggc gcgctgggag agctggtatt
    44401 tgcagggggc gccataggcc tcatgcccgg ccttgccctg gccgaacaga gccgccagca
    44461 ccgcgaaggc ggaggcggtg aaggcagcag cagggccgtt tttcaggcag acagcggacc
    44521 agcattcgtc cgtgacgcag atgttgatgt ggctgccgta ggagttgccg cgcccgtccg
    44581 aattgttggc cagcagccgt atcctgccgc cgccctccgc ggccagcccg gcattggcgg
    44641 cgagcatggc gcgccggcag atttcgaggc cggcgcgcgt ccaggtcaga tgatcgaggg
    44701 cggaggcgca ctccggcacg catatctcgg ggtggtcggc gtcaatgtaa caggagccgc
    44761 cattgccgag gaacaagcgc ccccagtcca ctgggtggta gtattgcagc ccgcgctggg
    44821 tggtgtcgaa gccgggcgtg tcctcgtcca gcgcgccaac ggggaacacc tgctggcccg
    44881 ccgccgcgcg aacagaggca tggacggacg ggatggcggt gacgaccccg ggcactgcgg
    44941 cgaggatgag gcgcgcgctg acggcgttga catcaagggc gtagtccgcc ggcaggccgg
    45001 tcatgaagtt ggccagttcg acatcaccgc cgatggtttt gggcactctg cggagcttgg
    45061 gtcgcgcgcg aaaattcatg gctgtgactc cgggtcgaga agttgaatga tcctgtcatc
    45121 atcctggatg gggcggggca cgatgagagg cggcccctcc gccgcgccgg gaggcatgac
    45181 cggatccaca ctgagcacct gggtggtctg cggcaggtcc gtgagatagt ggtggatgtt
    45241 ttcacgcgtg agcaggcgcc tcatgcgctc cgcagtttca tgcgcggccc attcgatgtc
    45301 ctcgacgcgg atgccgccgc cgccatcctg gatgaatcgg tcgtgggcgc ggcgtttggc
    45361 ggcgtcggca aggctggcga gcaggcgtcc gctgaccagc tcacgcgcga ggacgacacg
    45421 ctgtttgccg tcagtgaagg tcactttggc aatggctgac gcgggcgcgt tgtccgcata
    45481 cagcagggcg acggcggtct cgatcagggc ggagcgtgtg acatgccttt cgactccatt
    45541 gggggagaat ggcatgtgct caggcaggtg gactttgagc atctccgccg cggtgcgcgc
    45601 gtccgggcgc ggcacatgaa tgatcgagcc gccgatcctt tccacaaagg ccgggtcgag
    45661 catttcgacg cggttggtgg aggcgatgac ggccacgttg gacagactct gcagtccatc
    45721 cagttcggcc agcacgcagg cgagcacttt gtcgtcatgc acgccgccgg gggcggagcc
    45781 gcgagtcctg cccaggcact ccacctcgtc gaggtaaagc accacaagcc cccgggcggc
    45841 ctgggtgttg aggtgcttga aggcggcgcg cactctggcc tccgcctcgc cataccactt
    45901 ggaggcaaac tcacccggct tcaccgaggc gaatacgcag ttcccgtgcc ccgccttgag
    45961 ttccgccgcg agccgtttgg cgatcaatgt cttgcccacg ccggggggcc cggcaagcag
    46021 aacgcccggc cgcgcggaca ggccaaaacg gcgcgcgctg tcaccatgca ggaggcaggc
    46081 gcacagagtg gttttgatcc tctggtagag gtcctccatg ccgcccatgt cggacccgga
    46141 gacggaaggc ggctccttga gaatgctgaa atggtcgcca gcggccttgg gcagcagttc
    46201 gagaaaaatc cgggcctctc cgtggcagag cacttcgctt cccgcctcga tgcccgccac
    46261 agccgggtct tcgagagcgc cgggaggaac ctccagcagc tctttctggc ccgcgccgaa
    46321 ctccaccagc atcctcgcgc cagcccagcg gacaaacccg gccacgacgc agccgttcag
    46381 agacgcggcg catctgccga tgatggccgc acctcccttt gccagcaaaa cgccgtcacc
    46441 aggcttgagc gcgcgcgcga ccacgcctgc gtccacagcc acctgctgcc tgtgaccgcg
    46501 cgcaaacacg acggccttgt cattctccgc tgcgcggatg aagacagcct cgatcaacgg
    46561 ctcgccggtc agcagcttgt tctgctcggt gagccgctca ttgatcgccg ccatgcgccg
    46621 aacgcactcc ttttgccggc gcgcttcggc ggcgcagaga cgcagcagcg gggcgcgcgc
    46681 ctgcgcgtcg gcgcagctcg caagctgctc cacggagtcg gggtgcccgg gatcagtgag
    46741 atcggtggcg agtttgatga catcggcgag ttcctctcgg gaggggtgca tggtgcgtgg
    46801 catgggatac ggtggggggt gagattaatg gctagaccag acggtaaggc cgcctgacga
    46861 cgctgccgcc agcccagccg aaacaggcca gcgaggggcc gctgtcggcc aggttggcca
    46921 gcaggaagac ggagtaagcg cgcgggaaga ccgtctgaat catggccagg tccgccgcgc
    46981 tcagcgccgc gcgctggcgc ggg
//
LOCUS       DAOMYJ010000011        46908 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Prosthecobacter sp. isolate AMR_MDS_5629
            NODE_298_length_46908_cov_20.899836, whole genome shotgun sequence.
ACCESSION   DAOMYJ010000011 DAOMYJ010000000
VERSION     DAOMYJ010000011.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867850
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prosthecobacter sp. (wastewater metagenome)
  ORGANISM  Prosthecobacter sp.
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Verrucomicrobiaceae; Prosthecobacter.
REFERENCE   1  (bases 1 to 46908)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 46908)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.30
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:34:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,742
            CDSs (total)                      :: 4,684
            Genes (coding)                    :: 4,676
            CDSs (with protein)               :: 4,676
            Genes (RNA)                       :: 58
            tRNAs                             :: 55
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..46908
                     /organism="Prosthecobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_298_length_46908_cov_20.899836"
                     /isolate="AMR_MDS_5629"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1965333"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..929)
                     /locus_tag="PLP58_02040"
     CDS             complement(<1..929)
                     /locus_tag="PLP58_02040"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF019334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HRJ07334.1"
                     /translation="MPDDSPTDLTITEPSLAELGRALFTEAGVSARLPGHSWLPPAPE
                     ELQTQLPQFEILDMIGRGGMGAVYLATQSALDRLVAVKILSADLDALDPGFSERFKNE
                     ARVMAKLSHPGIVAIHEFGETASGLHFIIMEFVEGTNVQQMITASRRLRTEHALAITA
                     HVCDALHYAHERGIIHRDIKPANIIVGYDGSVKVADFGLAKVGVPGVETRQLTRSGTT
                     LGTPHYIAPEALVPGAPADRRADIYAAGVMLYHMLTGKLPQGLFELPSIQVPGLDPRL
                     DAIIARAMREDRDLRYPTAADMRADLDGILTQP"
     gene            1059..1802
                     /locus_tag="PLP58_02045"
     CDS             1059..1802
                     /locus_tag="PLP58_02045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HRJ07335.1"
                     /translation="MSEPHPNSHDLFPTTQWSVVLAARADGQSGRLALDELCRKYWRP
                     VRGFFGQCGVADDETEDLAQEYFASLLTREYLHQADRERGRFRAFLVQDLKFFLWDAL
                     AKKRVQKRGGGRAFVPLDETTDAAADSAGNTAFFDRQWALQIVENAREMLRAEHEAAG
                     KGELFRLLQLGLVRTMSAADYEQWAARAGMTPGALKVALHRLRGRFRRALEEQVRQTV
                     ATEQDFDDEMRHLRRALRDGFQDAPGDEA"
     gene            complement(1842..2813)
                     /locus_tag="PLP58_02050"
     CDS             complement(1842..2813)
                     /locus_tag="PLP58_02050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ07336.1"
                     /translation="MSLARALLGRGIGIIAPLWLIIAAAPLHSRDLEVIFIQGAAGED
                     EYAGIFQKQADAWREAVAKAGAAFTLIGQTSGGKDADLAEVESALKKAAQPAAGQLWL
                     VLCGHGTHDGREAKFNLRGPDLTPALLAQWLAPLKRKLVLIHTGSASGAFLKPLAGPR
                     RTIVTATKGGDEVFYARFGEHFAPAIAGLPEADLDQDRQVSVLEAFLHACKNVAEFYQ
                     NEDRLATEHALIEDNGDGTGTRSEVFEGTRPKSAPEGARPDGARAAQLVLVLSDEEAR
                     LTDAQRAARDTLEQQLEAHKDKRPTLSDDAYFTELEKLLRALAEVYH"
     gene            complement(2810..5182)
                     /locus_tag="PLP58_02055"
     CDS             complement(2810..5182)
                     /locus_tag="PLP58_02055"
                     /inference="COORDINATES: protein motif:HMM:NF018757.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine amidotransferase"
                     /protein_id="HRJ07337.1"
                     /translation="MNFLLQHPERLWITCAVMVLGFGVLLAGYLRSPLRGWRKWAAFL
                     CKLAALSLLALCVSDPLWTRQQPKKGENELAIVADNSASLTVAETRGGKTRAQSVKEA
                     LGGGADGESAWMGALSGIFRVKTLLADERLRGVADFGALDFSGRRSDLTRALKMLHEG
                     AAGRSLAAVVLVTDGHATDATSPGWTQGEGARPVFPVIVGDQAPAPDLAIQDVIVTQT
                     AFEDAPVMMTVKWDARGFDGREAAVCALDEEGRVLVSEKLKVLAKPPQAVARLKVPVV
                     KPGVSFLRIAVMESALLAKAARDEWRALAKEAVLSNNERLVCVDRGQGPYRVLYVSGR
                     PNWEYKFIRRALAGDDEVQVPSLIRIAKREPKFEWRGRVGESSNPLFRGFGDQSTAQR
                     YDQPVLIRLGTRDAQELSEGFPKSAGDLFGEYRAIILDDLEADFFTQEQMKLIERHVS
                     ERGGALLMLGGQESYQAGGYEHTPVGRMLPVYLDRVTSAPPVQNARFNLTREGWLEPW
                     TRLRPSQEEDEGRLAGMPGFHSVNQTFSIKPGASVLATVSTQDATYPALAAQRFGEGR
                     VVALTLGDVWRWGMGDADAMKDQERFWRQLMRWLVVDVPDRVQVAAEPLEQSVRLAVR
                     ARAADFLPMDDALVKLEVTGPEGGKSLLYGEPSLAEPGLFEAEYFPKTAGAYRVTATV
                     ENAAPPDESVEHTPFASSKSTGWVFQPQVEEMSRLEPARATLQRLADETGGRLLTLND
                     LDKLPELLSAIEVPVRETLSEPLWHTPWFFALILALLGAEWLLRRKGGMA"
     gene            complement(5228..5680)
                     /locus_tag="PLP58_02060"
     CDS             complement(5228..5680)
                     /locus_tag="PLP58_02060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0003953 - NAD+ nucleosidase activity
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="toll/interleukin-1 receptor domain-containing
                     protein"
                     /protein_id="HRJ07338.1"
                     /translation="MDFVSHSRRDFETEIHQVIRLSKRATVFLSCASADRATADALRR
                     VLRRHDFTVTSPEDSGASAQPIQDVIGTAINEAVERGFVLALLSPPGCGGVAAKQRPS
                     FPFPMAAAALAREAARWRLPGLACASFCRMAFFRLRIPLKTSPRFGVI"
     gene            complement(5811..6170)
                     /locus_tag="PLP58_02065"
     CDS             complement(5811..6170)
                     /locus_tag="PLP58_02065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ07339.1"
                     /translation="MLFFLKCMETTEADDRLLWLLIEREIKAREWFILCDSPNARNSA
                     AVLREMELVKSLAREGKVVETIDLDRDFSSTNGAPTSQPRPTPWVSCPTNCSSPERAA
                     QPTCTSPIRFAHGAFVT"
     gene            complement(6267..7286)
                     /locus_tag="PLP58_02070"
     CDS             complement(6267..7286)
                     /locus_tag="PLP58_02070"
                     /inference="COORDINATES: protein motif:HMM:NF025056.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003953 - NAD+ nucleosidase activity
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="toll/interleukin-1 receptor domain-containing
                     protein"
                     /protein_id="HRJ07340.1"
                     /translation="MLTRLRKWQWWTIQGLPPSPWRCPLFAPLDRAIFISYSRASEWG
                     GNLAHTLYERLREAGGEVYLDTLSLEAGGNWRRHLLRAINRSRVFISLVDSITVQREW
                     TNSETEAALYAWFQRCAPQIVLVLKPDSPLPSVGSGIMKPAFDLLLSSPQTQGLGGVR
                     ITQASSLTPVIFEEAFHKHRFGNTVSIFPKFLSFLLGLLNSPITALSRLMVYYGPIFA
                     GLQLQWHHGYGDLLALPLWKLLGCAFLLGFSGRELMNLTANILAGAQFRDFRASHISR
                     IRFLLKHLNPLSWALVLAYQFPLAAPLSIGWEAIFLVLGWASCTSWINFKLSQPVNGG
                     KLQPW"
     gene            complement(7413..8897)
                     /locus_tag="PLP58_02075"
     CDS             complement(7413..8897)
                     /locus_tag="PLP58_02075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HRJ07341.1"
                     /translation="MTRFDHYRQGVALQEAFYAEHPVPATGPDSASAELARQDERLQQ
                     AHQAYHQAILLCQAESAYRDQAFARRKLGQIYWAQNRLPEALQELTTAQNILDSLPEL
                     DRSDLAEESAVRFYLGQIARVQGRTGEAISCFERCVRIDTILYNVSDAIASMKQLERV
                     APNHPMAKTLDAVFRDTPPVAVAPSSPAPAEDRSSRIWGTSPSSRNIAILGTEPTALA
                     AMAQAAEEGGAMVDAKARSNPISILLGYLFFALGISRERGRRPHTETCDLTAADSNQI
                     RRVIRRSTAVLVVFQRSQFSLPGFQTAIQGIVKLMKQQDIFRAFIAASEAEIEWLKQQ
                     SAAGEPLANQILENIHLVPGQSKAELLQAINCYTSSIRQARWENRWRTWANAWAWLFA
                     GLAHLIVTIAPILVILTAAALLIRQEQTRPLVVPYSELLCWMMGLNVCVFQMPVFYVA
                     GRAFSRTLKGSGVSYSWLPSPAAFGGNRLFVWMFLFLAIWLPGQ"
     gene            complement(8884..9999)
                     /locus_tag="PLP58_02080"
     CDS             complement(8884..9999)
                     /locus_tag="PLP58_02080"
                     /inference="COORDINATES: protein motif:HMM:NF025056.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003953 - NAD+ nucleosidase activity
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="toll/interleukin-1 receptor domain-containing
                     protein"
                     /protein_id="HRJ07342.1"
                     /translation="MNSPHCQSAKTMPIPESAAFLCYDCRREFTPDKVATPLRIFLSY
                     GHDANEELVRRIKADLEERGHDVWFDQNEIKAGDHWRRSITDGITGSQKFVAFLSKHS
                     TRDPGVCRDEIAIALGVKGGNIQTILVESEAEVQPPVNIGHVQWLDMHDWKERQERGS
                     GVSPENLDSWETWYQGKLAEIVRVVESDESRRFAGEIETLATHLKPIRSDARIYDLLK
                     KGFFGRQWIFDAIETWRNPGNAGPQTGSSPFDIRNSSFETPSRLFWITGDPGVGKSAF
                     AAQFTHTRSDAVIAAQFVEWDKPDHRDPRRVIRSLAFQLATRLPDFRKLLLTLPEIAE
                     LDRKDPAELFDYLLANPLALSLSGSMHPQIRNRHDSI"
     gene            complement(10123..10581)
                     /gene="ybeY"
                     /locus_tag="PLP58_02085"
     CDS             complement(10123..10581)
                     /gene="ybeY"
                     /locus_tag="PLP58_02085"
                     /inference="COORDINATES: protein motif:HMM:NF014216.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - ribonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HRJ07343.1"
                     /translation="MRTTPRITLSDQQRSPRPPLPWLRRVVKAAMPACIAAVKSSGAP
                     LTGLEEIEITLISDAEISRVHAEFLGDPSATDVITFHHGEILISADTALRQAAAHGKS
                     LDHETALYAIHGLLHLAGWDDHDPAEAAAMAALQEEILSRAVREVSTAGI"
     gene            complement(10578..12206)
                     /locus_tag="PLP58_02090"
     CDS             complement(10578..12206)
                     /locus_tag="PLP58_02090"
                     /inference="COORDINATES: protein motif:HMM:NF019318.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDIG domain-containing protein"
                     /protein_id="HRJ07344.1"
                     /translation="MRRKHQEGDLLDELRTQPVVTFLLFLGFFLITGVLMQAGAAVIP
                     GTVAPQPLAYLLAVALVTAMVVHLRVSLIEDVEDNAELLLVLGTLLLQFAGHVLMLKF
                     GHREGWTGALLVMALPHAFAPLVLSVTLGRRIGLFGAIYGTLLGTLVCVAVNPLIYLA
                     ASVLIGFLVVLFTRRVRRRNRLFASGLYAGALAALFSLCLHMTTDTPLDSTLAAQVLG
                     VPFAVGVITGLLVGGLLPVIESLFGVTTEISWLELADLNHPLMKRLSMEAPGTYHHSL
                     VVANLSEAAAEAIGANAAMSRVCAYFHDIGKLAKPEYFIENMDPADNPHDDLTARMSA
                     LILIAHVKDGVDLAIKHKLNCSVIDVIEQHHGTSMTQYFYNRALQQQAEVQRLVGEGK
                     AKEDDVPKVSPEVFRYPGPVPQFKESAIISLADAVESASRAMEKPNASRIETLVDELV
                     LDRMKDGQLDECDLTISDLARVKASFVKTLLSMMHSRIKYQKTAESGIPPAAPKPEVH
                     DGLTIVMDRPGAGGDTATGEFKKRSARKPPASAA"
     gene            12486..13424
                     /locus_tag="PLP58_02095"
     CDS             12486..13424
                     /locus_tag="PLP58_02095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009960270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate isomerase"
                     /protein_id="HRJ07345.1"
                     /translation="MPRKLSHIAPDWWDYTTLDPELINDAARLTERDLRQLSRPGFKV
                     VMYDSLEDFYLAEALEYIHAWKQATPDNPAGICGPIGPTEQLPLVARLVNDLGISIKD
                     GHFWGMDEWYDPETKKEVSMDHPLSFEKADRELCFNRIQKKLVMPDSQLHFPKADVTA
                     YLKSWQSGVRCVVMQGGQGDIKHWAFNDPLKRAGKWKNEPPPPAEYRKLGTRIVELHP
                     VTLSQNARTSGGGNITMVPQMAITVGPKETWMAEKVSIWQAGMHDNPLGQRLTALMIS
                     KRLADSSVPMSLLADHQNVQFNYYRGGLGSCAVEMH"
     gene            complement(13478..16141)
                     /gene="gyrA"
                     /locus_tag="PLP58_02100"
     CDS             complement(13478..16141)
                     /gene="gyrA"
                     /locus_tag="PLP58_02100"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012420326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006259 - DNA metabolic process [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HRJ07346.1"
                     /translation="MQDSNTRPISVADEVKNSFLDYSMSVIISRALPDVRDGLKPSQR
                     RILYAMHELSLYPPKKHVKCAKIAGDTSGNYHPHGEAVIYPTLVHMGQEWAMRETLID
                     PQGNFGSVEGDPPAAMRYTEARMTHLGGALMQDMDKDTVDFVPNYDERLTEPTVFPAA
                     FPNLLVNGGTGIAVGMATNMPPHNLGEVVDAVCAQIDNPAITTPELMAHVKGPDFPTG
                     CTIMGTSGIREYMETGHGSVRIRGQAEIVENGNREQIIITEIPYNVNRAELEKKIAEL
                     ANEKIIPEISGVRNESDENSRLVVDLKKDARAQVVLNNLYKHTELEASFSVHMLAIDG
                     NRPRVFGIKDAIGCYIEHRREVIIRRTKFLLGKAEVQAEKLEAFLLALGHLDDFIKII
                     RDSRNRDEARAGLKAYTFAVATAEQLGILIRSQPSIQDGRYVFTDKQVDDILELRLYQ
                     LTGMERDKVKSEYDAILATIKDLLDILANEHRVLTIIKDELHVIKAKHATPRLTRIEA
                     VSGEIESVDLIPNEAAIVTMTHMGSIKRTPTAEYRLQGRGGKGLKGMETREAQSEEDA
                     DDFVEHLFSAQLHDFLLFFTNTGRMYVERVYQIPQATRTGKGRSIKNVLNLRNEEKIA
                     SVLILKAQGAEDEAMWAADRYVLFATKDGTIKKTALEEFKNYRKDGIIAIKLDEGNDL
                     VDVVLTDGTKEVCLVTHEGMCVRFHETGDDPASPNLRPIGRNTAGVRGVMLDADDFLV
                     SCLTNEPETMVLVVSENGLGKRTPFQEYRMINRGGKGVITMNVTEKTGKVVAALAVHE
                     SDELMLMTSKGQSVRIKVSEIRETGRNAQGVKLMTLNEGETIQDVALVASEDEDESAV
                     ADTGAGDPDGVDAAATDDADPGSAGDVTASS"
     gene            complement(16188..18779)
                     /locus_tag="PLP58_02105"
     CDS             complement(16188..18779)
                     /locus_tag="PLP58_02105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009963256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit B"
                     /protein_id="HRJ07347.1"
                     /translation="MPDDIIDSTGSNSTAVAADQAYGAEQIQHLEGLKAVRMRPGMYI
                     GETDERGLHHCVFEVVDNSIDEHLAGFCKNVWISIHSDGSISIEDDGRGIPVEMHAKG
                     MPTVELVLTNLHAGGKFGDGAYEFSGGLHGVGAKCVNALSDWFKCEVYRDGKIHAIGF
                     ERGITTEPLKVLGDLDDPKRTGTKISFFPDATIFTITTTFVFERLRTRLRELAFLNPG
                     ITIHLTDERSESARSETLLYTEGVKQFVNELGEGKDKVHPEPIVIAGRREVVLEDKNK
                     FILVDCVMQYHKSLGEHTLCFANAIPNPDYGTHYTGLKTALTRAVKKYITDHPEQYGE
                     KGKTKAPIKDKAKWPELDIDSDDCREGLTCVLSVKHPHPRFNSQTKVKLVNGEVEGVV
                     SSVVYEGVLHHFDHNPDVAKAIVDNIIIAAKSRLAAARAREAIRKDAMSSGGLPGKLA
                     DCSERDAAKTELFIVEGDSAGGSAKMGRNRHNQAILPLRGKLINTEKARLEKVLANKE
                     IQTMITAIGTGIGGDSEVEGSFNIEKLRYHKIIIMTDADVDGSHIRTLLLTFFFRQMP
                     ELVKRGHIYVAQPPLYQVTRKKREEYVQNDEEMDAILLELGSGEITLRNVSDNTKVDS
                     DKLKQILGLLVPMAKFADIIRRHGADFETYLQHRNTETGELPHYLVIIREGNEVNVKY
                     LLDRDTLANFARENADLHLFGKPDVEGANGTAAPKQTRRARLIEVHEAHGMKARFKQL
                     DELGLHVDHFSSQDKPLFEVIEGDGDNAKVHPVFSIPGILDKVMEIGKRGMEIKRFKG
                     LGEMNAKQLFETTMDPTKRTLLQIKLDESNAHEAERIFTILMGDVVEPRKHFIEENAL
                     NVRNLDV"
     gene            18957..20387
                     /locus_tag="PLP58_02110"
     CDS             18957..20387
                     /locus_tag="PLP58_02110"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018968883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HRJ07348.1"
                     /translation="MPLENPFDFIVLGGGSAGYAAARTAHSLGMSVAVVDGAAELGGL
                     CILRGCMPSKTLIESADRNLTLRRAAEFGLVASPGAPDIRAIRDRKRALIADFAGYRR
                     GQLEDGRFTLLRGRGVFKDARTLVVSPLDGAPTFEVAGRSFCIATGSVVNVPDVPGLR
                     EAGFWTSDDVLDAEELPGSFLVLGGGAIALEMAHYLEGMDRRVTVLQRGPGFLSGLDP
                     ECGETVRKAYEARGITCRLGARLLRVERAAEGAKRVVFEQGGHELGAAAEEVLVALGR
                     SPAVEGLRLEAAGVALHGKKIAVTGAMQTSQPHIFAAGDVCSPLDVVHVAIQQGEAAA
                     RNARRVLQGVQGLESVDYRLLLFGVFSHPQVAAAGQSEAALARAGIPFVSASYPFDDH
                     GKSMCMGETEGFVKMLAHRETGEILGASVAGPHATELIHEVVAAMHHRCTAHEFMSIP
                     HYHPTLSEIWTNPAEECAELLDPSAA"
     gene            complement(20400..23165)
                     /locus_tag="PLP58_02115"
     CDS             complement(20400..23165)
                     /locus_tag="PLP58_02115"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HRJ07349.1"
                     /translation="MKPSFSISGRFMVFGVLAVVAAAAVTAAAFLWQEMLILRAAQRA
                     YLGSIVQAAQAELRSQGVSPGALASTKDLTSEAITHLKMRLQVVDVPPMIQDLGLRIE
                     IAGPNARNGEPPVETSPYILVHDTALEWQPPEPEFAQSRDIIFRVLAGSSVVVGDDPL
                     SLRADLDGPGGWVIAGAPLINPQGVADGVILARQPRIHRRHIINLPRLMVPIFGAVAG
                     LLPAVLGFYLLGRAISRKTTALRQGFEALSRGDLDHRLRVTGSDDLDTLQHQFNQAIA
                     TFQQEEARRQHLVAEFQRARKQAEVATAAKGDFLANMSHEIRTPMNGIIGTTSLLIEM
                     GLAPEQEDLVRMIHSSGVSLLHLINDILDFSKIENAKMEIEDMPVEMEKLLAEVTDVF
                     SYRAAEKGLELNFHADPALPRFFRGDFQRVKQVLVNLVGNAVKFTDKGEVLILARHVS
                     RKTRNGEMPHLHFSVRDTGPGIPADKIGTLFQAFTQADSSTTRKYGGTGLGLAISRKL
                     CHLMGGEISVISEEGQGSDFFFELPLRVSADEAGREQELAWETAVKGLRVVFHTPHQT
                     TRQILQQHFQEWGVIPIHAPAASAREVAADLDEKSLFMLDASSHDPETAAPLIQAAAG
                     RRAGVLVLLPLTRWKLRDQFAPRIPARFCKLAKPAKHRELLRVLAELARMPQPAPTTP
                     LSSPSTAPAAQVPAMHAAQLIAHNPAAMNAQPLPPAFPGNDPAWLQPPVPEPVAPAAL
                     RGVFHEDQPATGSLNRAAAAGHDAVISKDTNRAIAAAARAGGETFASQHPARLLLVED
                     QPLNQKIAVMLLQRLGYEQVDIANNGQEAVEMVGRGSYDIIFMDLQMPVMGGIDAARA
                     IRGNFHLKNQPAIIAMTGHALTGVREECKAVGMNAFLTKPVSLEDFRKAMPPCLEKGA
                     AMRPMTL"
     gene            complement(23172..23594)
                     /locus_tag="PLP58_02120"
     CDS             complement(23172..23594)
                     /locus_tag="PLP58_02120"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HRJ07350.1"
                     /translation="MNIHSHPHRTTGKVLVVDDEPQVLAVAKAILDAHGFEVIVTDSG
                     DVALNLISQPAPPDQRIELVILDLTMPGGLSGFEVLEEMRRMDPALPIIACSGYFQEN
                     ARDLCQAIGFSDILPKPYTLDHLVATVRGSLVRSHQLA"
     gene            complement(23619..25787)
                     /locus_tag="PLP58_02125"
     CDS             complement(23619..25787)
                     /locus_tag="PLP58_02125"
                     /inference="COORDINATES: protein motif:HMM:NF012652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GspE/PulE family protein"
                     /protein_id="HRJ07351.1"
                     /translation="MSAVVLASTPASSENFGQSKIPPAATIDAKQAAILSIEGLALQY
                     NMLPVNLLRESCSPEAEKIVRKLGRVPYVADPWLPVTTMGPLLVMAHHNPRAGDLWGV
                     PPFLTLRVLITPDQYQKTRKDLVQRFGQLPIAQQNQMEGVQAPRFADMGLEGAFEWLL
                     ASYPYEPAEVTKLRGFFETQKEKHSNLGVSHFNTVQRHLGVVLQHLVSGGRTLCFSPA
                     EAQRQTFFPLPLLERHTVYPLYIGKNAVFMFSESGDCYAFEDEWLSMGNAAVKIVPVQ
                     ADPAAIRDAINRAGATFDSGGVAKLDKSTLTASVSGNESVIDILPDEMGRVNPASPNH
                     SAEELVQWALFTSIRCRASDLHLEKFYNLARFRARMDGNMKTILTAPESMLNRFVALL
                     KNYAGMNQSRQECQDGRFAVSVGARRVDIRVAAVPTRREFQKVIMRFLDKQDGVKRLS
                     DFNLGQRQIDILTRVMQRDQGLVLVTGPTGSGKTTTLYALLNSVNDDGVNIQTIEDPI
                     EYEIEGINQTQTNPHYGLDFANGLRALLRADPDIILIGESRDAETANAAVNAALTGHL
                     VLTTLHANDSLRAVSRLMSMGVEKYLLADSLALSQAQRLVRRLCSYCKQPMQVPDDIQ
                     AMMARQGVISQPLVQPIYTAGGCQECHGTGYAGRVALMELNEISTELRDLIEEGAPMS
                     AMRACAFKNGFFSLYQEGLMQVIAGHTSLDEIKCLAYTAA"
     gene            complement(25803..26327)
                     /locus_tag="PLP58_02130"
     CDS             complement(25803..26327)
                     /locus_tag="PLP58_02130"
                     /inference="COORDINATES: protein motif:HMM:NF019575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HRJ07352.1"
                     /translation="MPFAPPAAAARAPRFNGRAAGYSLSELVITISIMSVLAGVAILS
                     MNSAYSAGQETLAMERVEMLNQGLHRWSAANNEMSFIRRDDSSADELKVLRDLQYRNP
                     IEERAVIGAPFVPPEYNPESSSDSGAYRIRWNSRGYELLRPGQEGTGLLMVFDGSDFT
                     EAFQFPENYQSSGR"
     gene            complement(26353..26748)
                     /locus_tag="PLP58_02135"
     CDS             complement(26353..26748)
                     /locus_tag="PLP58_02135"
                     /inference="COORDINATES: protein motif:HMM:TIGR02532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HRJ07353.1"
                     /translation="MKHPASKSRRGFSLVEMMACVSIIGIVAFLAIPSVTRMRSDSER
                     NLAISRAEALNLAQATLIQVRGRTQAGLDWTGAASNEAKFNLLRPYLSYAENTLSLYM
                     PSGYTVTLPSGITTMQKSTLTGPSGVIPY"
     gene            complement(26759..27241)
                     /locus_tag="PLP58_02140"
     CDS             complement(26759..27241)
                     /locus_tag="PLP58_02140"
                     /inference="COORDINATES: protein motif:HMM:TIGR02532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HRJ07354.1"
                     /translation="MRTPTPSKRGFTFVEAVFTIAIIGIMSALAVSAISNGARDANRV
                     VARQQQAALQEALHVWVMAQTRNATTGQVQGLGSIRATYNALATTSARFNLLLPNPSA
                     VDVSARSGFLDQTTADHFLEYTTGTDRLKTAALSGAKQHLTLPAWQDGDLPRVELVND
                     "
     gene            complement(27389..27787)
                     /locus_tag="PLP58_02145"
     CDS             complement(27389..27787)
                     /locus_tag="PLP58_02145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007414772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HRJ07355.1"
                     /translation="MKNINIRSGLKAGFSLVEMLVVIAIIGIIAAIAIPNIGNINDSA
                     AQATAKRNAQSVASVMNAAIAAGYNASWADKAAVLTAAQGTAVAPSSGPFKDKVFTVG
                     DIDDEQEATVLSYLDWDATNKQVIYKANGS"
     gene            28026..29309
                     /locus_tag="PLP58_02150"
     CDS             28026..29309
                     /locus_tag="PLP58_02150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009958251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HRJ07356.1"
                     /translation="MEPNLKIALVGAGMFGGDVHLRAYADLQRFGIAGQLARVGLDKF
                     SRDLAPVKFDLVAVATRSQKSAAKSAAAFKEWTGHEPKAYFGDTPWDDILRDFPDLDV
                     LAVATPDHLHTQPILAALAKGAHVLTEKPMCLSIQEADEIIAAAEAKNCVVAVDMHKR
                     YDPDHLRIRDDIQKRIGAPLYGTAYLEEPLEVSTSTFKWVESSDPFSYVGPHWTDLIW
                     SYYKSKPAALTAVGQKKRLVRDGINAYDAVQVRVDFDNGMSIAFNNNWITPADFEGPV
                     NQGHEIVGADGKVESDQQYRGFRFWNAGGGCRTSNNHFTRDVLRADGTRAYIGYGVDS
                     LTVGLAAICRVKFAGESRAAVADMYPTAEDARITCAIVDAAAKVRDLNFKYLQEGKGA
                     PVTARFGQDGITLIDPCRAAEGPDMVFQRIYKGAI"
     gene            29325..29855
                     /locus_tag="PLP58_02155"
     CDS             29325..29855
                     /locus_tag="PLP58_02155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ07357.1"
                     /translation="MSEPPDEKEDSEEDDLAEEDFEDDVTDEELLQQMRPWHRALMVF
                     LVVPLIKTYDFLEGFFRTKWRIIPLDELPAARQRWSSVSMTPEQVRDLIKRQDGFLCD
                     ECKMELKTQRQDAEELFPNVWEVRKHGRCEACGADREHCSRIHHGYLLFKEDGRWMAV
                     FAQVPFLRRCWLRLRG"
     gene            complement(29993..31207)
                     /locus_tag="PLP58_02160"
     CDS             complement(29993..31207)
                     /locus_tag="PLP58_02160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ07358.1"
                     /translation="MKARTGKIARLPKAVREQLNQRLADGEPCGPLLAWLHSLPEVLE
                     VLVRDFAARPILKQNLSEWRQGGFLEWEARQERLARLRLLADEADELEEASPRLPDRL
                     AALLAGRFASVITTMQVKDWSKPRQLARLMELNEAVATLRRFDQGAARLKLEGEQLEI
                     KKAELQVAREAQTKRTHEQWREWAREQMQRLFHESKTPGELTKAVNHLIFGEEDEDKR
                     GWQENAGSTGTNLGAQMQEDAAGLIKLRDEIKAEKAGIRFCENQIKMRQAEAGDAIRE
                     ALWDVLSLEQPDFPDAEAGTDHQPASPKESQTQSNLVKPTRAPPVSTAAAGLPAAGVD
                     PVEEESNEAVITHPPKGKAGNEEAASFAGEPPETPDEDPGGDPLEPDVLRSGSVTVLL
                     EQPSRLKPGRLC"
     gene            complement(31212..31406)
                     /locus_tag="PLP58_02165"
     CDS             complement(31212..31406)
                     /locus_tag="PLP58_02165"
                     /inference="COORDINATES: protein motif:HMM:NF025005.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KTSC domain-containing protein"
                     /protein_id="HRJ07359.1"
                     /translation="MIKLNSDAIKAVDYDPARRLLHIWFPNNGPYTYYGVPERVYLRL
                     VNAASPGTFFHEVIKKNYAL"
     gene            complement(31455..32450)
                     /locus_tag="PLP58_02170"
     CDS             complement(31455..32450)
                     /locus_tag="PLP58_02170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ07360.1"
                     /translation="MNGTDPPDHLPAFRNYGEAVAMAKQSGDAFYVIAFLLEAWLGDA
                     SDAALAEYAERKGKDRRLQTGRAWESWQQLFGKAREDELPGILECIGRYSNCDAPESE
                     LVGRALHLMRLEDELGEPVSISARRKAAEEKSMDFKMCLKHLRYWFQRFAEWQEALAH
                     WQAHWVAHMAPLALQASPERRELVQLGLIQRNFADLNPHDKDWWQFRHEELAAQHQGD
                     KALGLIGKAQSNEKWGALKRTQVDELVIHWWPLLLRHGWTDRDVRLLLREVVDRPEEY
                     PLQEDRELADYRQKALGLKKNNARQDKSAPDGRPRGWRVALAMVDRAGADSSESK"
     gene            33057..33362
                     /locus_tag="PLP58_02175"
     CDS             33057..33362
                     /locus_tag="PLP58_02175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease signature motif containing
                     protein"
                     /protein_id="HRJ07361.1"
                     /translation="MTEAEKIQLVWNKGAIPIPQEAANWRKDTCGAWIARNQYGNRDS
                     NYGWEIDHINPNGGDAISNLQPLQWENNVAKSDGRHKCVVKGVGNKNFRQPEVTISR"
     gene            33359..33943
                     /locus_tag="PLP58_02180"
     CDS             33359..33943
                     /locus_tag="PLP58_02180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ07362.1"
                     /translation="MTPIPLHHIADVRMGVTLRGRDATRPALHGSCFMIRISDLSDDG
                     HLTTEDLHQFEPGEDIRPDHFLRPGDVLLPNRGTRCTAYVYDLPLTNVLVGAQFYIVR
                     PDTTKVSAEFLAWFLRTEKAAAHFALRRKGTLVQTVQRKDVLELELPLPPLAKQHSII
                     ALDALAIQERQLSIQLAEKKAAYLQRAMLHAASA"
     gene            34069..35559
                     /locus_tag="PLP58_02185"
     CDS             34069..35559
                     /locus_tag="PLP58_02185"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007418948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009007 - site-specific
                     DNA-methyltransferase (adenine-specific) activity
                     [Evidence IEA];
                     GO_function: GO:0015666 - restriction
                     endodeoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0006306 - DNA methylation [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction-modification system subunit
                     M"
                     /protein_id="HRJ07363.1"
                     /translation="MPKVNQSEINDVAWRACDTFRGVVDAENYRNYILVMLFWKYMSD
                     VWRDHRDAWLKEYNGDEERVKRRLARERFQLPDGCDFYSLYAQRNEADIGERMNVALA
                     GIEEANKAKLEGVFREVDFNSESKLGQTRDRNARLKLLLQDFADPRLDLRPSVVGEED
                     VIGNVYEYLLERFASDAGKKAGEFYTPGQVSSLLARLLAPKKGQTICDPTCGSGSLLI
                     KVGRQADERDFALFGQEMNGTTHALCRMNMFLHGMDNFRIEWGDTLRNPRLTEHDQLM
                     KFDVVVANPPFSLDKWGHEDAEKDPFHRYHRGLPPKSKGDWGFITHMIETARERTGKV
                     GVVVPHGVLFRGAAEGKIRQRMIEENLLEAVIGLPANLFFGTGIPAAILLFNKGKTHG
                     DVLFIDASREYKEAKNQNILTEEHVRKIVDTYAAFQTVEKYAYRATPQEIADNDFNLN
                     IPRYVDTFEEEAEIDLDTVKADITRLESELATVRGKMQAYLEELGV"
     gene            36512..37531
                     /locus_tag="PLP58_02190"
     CDS             36512..37531
                     /locus_tag="PLP58_02190"
                     /inference="COORDINATES: protein motif:HMM:NF013579.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HRJ07364.1"
                     /translation="MTIVGAGTGNVALVPDYVSGANITQTTARIAVDQSLADTNYVRH
                     QLQSQTGKREVYRYVKGGAQPGLNIGDVEEFLIPLPPLPEQRKIADILTTWDEALTQL
                     DALIETQERRKKALMQQLLTGRRRLKGFDQSNGKTASDRFGTYPADWKRVRLGEVTTE
                     VPTKNAQGTKLPVLSCTKHRGLVLSEEYFGKRVYAEDTSGYRVVQRGEFAYATNHIEE
                     GSIGYQNLCDAGLVSPIYTVFKTTEAVDDSYIFRVLKSTMLIHLYQINTSASVDRRGS
                     LRYDEFSRIHIWLPGKEEQTAIAQVFDTADQQLTLLRTQRSALDQQKRGLMQRLLTGK
                     LRVKP"
     gene            37978..38733
                     /locus_tag="PLP58_02195"
     CDS             37978..38733
                     /locus_tag="PLP58_02195"
                     /inference="COORDINATES: protein motif:HMM:NF014691.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HRJ07365.1"
                     /translation="MKTFPDIDRLKATLDQLRPLDPAIVRNLREDLIVRWTYHSNAIE
                     GNTLTLQETKVALEGITIGGKSIREHLEAVNHKNAILLLEDLVQKNEPLTEWTIKSLH
                     QLILKGIDDDNAGRYRTVNVRISGAEHLPPDQVLIPELMENFITWYQTEAMALHPVER
                     AARVHSDFVKIHPFVDGNGRTGRLLMNLELMKAGYPPAVLPVDVRLAYYTFLDADHVR
                     GEKDYFVGMVSRIVKQGFRPYFHALSLPWEDGV"
     gene            39195..39647
                     /gene="tnpA"
                     /locus_tag="PLP58_02200"
     CDS             39195..39647
                     /gene="tnpA"
                     /locus_tag="PLP58_02200"
                     /inference="COORDINATES: protein motif:HMM:NF033573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
                     /protein_id="HRJ07366.1"
                     /translation="MPQSLAKLYVHLIFSTKNRERVLMDDIRHDLHAYMGGTLKGMDC
                     LPIEINSEPDHAHVLFLLGRTMALSEVVGGLKKSATEWLRERGEAYRGFHWQAGYGAF
                     SVSQSAVEDVREYIRNQREHHGVKTFQDEFRAFLRKYEIEFDERYVWD"
     gene            39972..43241
                     /locus_tag="PLP58_02205"
     CDS             39972..43241
                     /locus_tag="PLP58_02205"
                     /EC_number="3.1.21.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007418944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019812 - type I site-specific
                     deoxyribonuclease complex [Evidence IEA];
                     GO_function: GO:0009035 - type I site-specific
                     deoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HsdR family type I site-specific
                     deoxyribonuclease"
                     /protein_id="HRJ07367.1"
                     /translation="MPPSFQEALISQIPALRMLQHLGFTYLSPEECAVERNGRLGRVI
                     LEKVLAGQLRRLNKISFKGREVAFSEENIARGIESLRDLPFEGLVRTSEKVYDLLTLG
                     KSLDQTIGDESKGFTLRYIDWQNPRNNVFHVTAEFEVERSGSKATRIPDIVCFVNGIP
                     LVVIECKRPDEKDSLTDAMNQSIRNWQADEIPQLFLYSQLVLGLNKNDGSYATTGTPL
                     KFWAKWREMPDATDTVRRVINTPPRPEDHAKLFKGLFAYAREAFEEQMMHEREPTEQD
                     KLLWSLCRPERLIELARQFILYDEGGAVKKVARYQQYFAIKATLGRVRQRDVQGRRKG
                     GVIWQTQGSGKSLAMVLLGKALALDESISNPRIVIVTDRIDLDEQITKTFHQCGKDPQ
                     QAKTGAHLIELLRDERVAVITTVLFKFEAAAKVARELHKLKDDLFVLVDESHRSQYGD
                     ANIQMQKALPNACYIGFTGTPLMKKEKSTATKFGGFIEPAYTIRDAVDDKAVVPLLYE
                     GRHIVQEVNQKPVDTMFEAMCEGLTKEQKADLKRKFTARDELNRLHSRLYLIAWDVSQ
                     HFSKQWKGTGFKGQLTAPGKKEALLLKTFFDQFGKVSTEVIISGPGEIENAEEHQTAE
                     KDESVELFWKAMMAKYGTEKEYNRRLIDAFKKGDEPEILIVVDKLLTGFDAPRNVVLY
                     IARSLKGHTLLQAIARVNRLHPGKDYGYILDYYGVVTELHEALELYSSLEEKFDQEDL
                     EGTLTNIAEEIKKLPALHDALWDLFKEVKNKKDEESFEVALADKDKREDFYTRLWSFC
                     RVLKMASSSIHWVTETPAEQVQRYKADAAFFQKLRASVKLRYAEEIDYRDYEKQIQKM
                     LNTYVQADEVIQVVEPVNIFEREAFEAEVDKAKSPRAKADTIANRTKKTITEKMEEDP
                     FFYRKLSALLEQAIADYRAERISEAEYLQRVTAVMAEVRGGNTKDVPDVLKQNDLSRA
                     LFGALKEQIAPKAGTSGEDTGSVLREDGPAYGGEAQRPAPPADEILAEAAVSMDQIIR
                     KHAVVRWRENIDAQNRMRNDLDDFLFDLQKEKGFTLSYAQMDAIIEAVIRIAMHRTDD
                     V"
     gene            43234..43932
                     /locus_tag="PLP58_02210"
     CDS             43234..43932
                     /locus_tag="PLP58_02210"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013980.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family zinc-dependent metalloprotease"
                     /protein_id="HRJ07368.1"
                     /translation="MSRPEIIDTPAGPAQLKRSHRKTLAISVLPDGTLELVAPDHAPL
                     EAILPRVAKRLGWIKTQRRAFREMNATRPALRFVTGATHRYLGRQYRLKIAKGEAQEV
                     SLRGAFFHVSIPKTGENEVKQALEAWYRRQSRLQFERRLASWSEWCRHRRLPQPKLRL
                     RRMPKRWGSALPDGTICLNPELIQAPSACIDYVITHEICHLKHPDHGPKFRALLQQLC
                     PDWKRLKERLESTG"
     gene            44291..44896
                     /locus_tag="PLP58_02215"
     CDS             44291..44896
                     /locus_tag="PLP58_02215"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009963201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HRJ07369.1"
                     /translation="MRAFSLAFPARLWFMRGMNELEANPWTTLETRTAYANPWIRVRH
                     DEVLTPAGKPGIYGVVEYQNRAVGVLPLDEEGNTWLVGQWRYCHESYEWEIPEGGCPP
                     GENAMECARRELLEETGIVAGDIEPLLTGLQLSNSTTNEVCDIFLARVAGFGEARPEE
                     TERLQVRKLPWREALAMALDGRIRDSVSVLALLALANRGGR"
     gene            44907..46163
                     /locus_tag="PLP58_02220"
     CDS             44907..46163
                     /locus_tag="PLP58_02220"
                     /inference="COORDINATES: protein motif:HMM:NF012427.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HRJ07370.1"
                     /translation="MLTPFGSAGDVFPLIRLGRLLRARGHRVTMVTACIFEKAARDAG
                     LEFVPLGKPEDFERMIADPRVWRLGSGTRLVFEFAGHAAGAYLAEIERLAQAGDRPSL
                     LLAPCTAFGARLAREALGIPLVTVHLQPAVFLSAHEPPVILPGMEHLRRLPLWMRRLL
                     LRAPNPADLFAGPLVRALCARRGVPPPRSLWWDWGNSPDGVLALFPEWFAAPQPDWPP
                     GVFQWDFPLEDLSLEHELQPELREFLAAGEKPVVFTPGSANIQARRFFEAALEAVTRA
                     GCRAVFVTRTLAQVPARLPPSVLAVEYAPFSALLRHAAAFVHHGGIGTLSQGFAAGLP
                     QVVMPMAHDQPDNAHRLEQLGAGLSLPPRRFTPARVAAALDECLRNQRLIESARQCAA
                     RLHPRADSRPMLEWLENRASPQEGRP"
     gene            46241..>46908
                     /locus_tag="PLP58_02225"
     CDS             46241..>46908
                     /locus_tag="PLP58_02225"
                     /inference="COORDINATES: protein motif:HMM:NF014234.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004665 - prephenate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_function: GO:0008977 - prephenate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0006571 - tyrosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase/arogenate dehydrogenase
                     family protein"
                     /protein_id="HRJ07371.1"
                     /translation="MPDFSTLTILGPGLLGGSLALAIQERMPGARLRLWARRDSAEAE
                     IRARGIHAEFFTDATAACHGADLVILATPVETMPALAARIALADLPAHALVTDVGSVK
                     GSVVGELAPILGGRFIGSHPMAGSEKTGIETARADLFEGAACLLTPLEGQDAAPLRAF
                     WQSLGCRVLAMTPEEHDLKVARISHLPHWMAVVTTLAALRADPDAADSSGWFSPSHHQ
                     RPIR"
BASE COUNT         8732 a        14335 c        15229 g         8612 t
ORIGIN      
        1 atgggctggg tgaggatgcc gtcgaggtcg gcgcgcatgt cggcggcggt ggggtagcgg
       61 aggtcgcggt cctcgcgcat ggcgcgggcg atgatggcat cgagccgggg gtcgaggccg
      121 gggacctgga tggagggcag ttcgaagagg ccctggggca gcttgccggt gagcatgtgg
      181 tagagcatga cgccggcggc gtagatgtcg gcccggcggt cggcgggcgc gccggggacg
      241 agcgcctcgg gggcgatgta gtgcggggtg ccgagggtgg tgccgctccg ggtgagctgg
      301 cgcgtctcca cgccagggac gccgactttg gcgaggccga aatccgccac tttcacgctg
      361 ccgtcgtagc cgacgatgat gttggcgggc ttgatgtcgc ggtggatgat gccgcgctcg
      421 tgggcgtagt ggagggcgtc gcagacatgg gcggtgatgg ccagggcgtg ctcggtgcgc
      481 aggcggcggc tggcggtgat catctgctgg acgttggtgc cctcgacgaa ctccatgatg
      541 atgaaatgca ggccggaggc tgtctcgccg aactcatgga tggcgacgat gccggggtgg
      601 ctgagcttcg ccatgacgcg ggcttcgttt ttgaagcgct cgctgaagcc ggggtcgagg
      661 gcgtcaaggt cggcggagag gattttgacg gcgacgaggc gatccagcgc gctctgggtg
      721 gcgagataga cggcgcccat gccgccgcgg ccgatcatgt cgagaatctc gaactgcgga
      781 agctgggtct gcagctcctc cggggcgggg ggcagccagg agtggccggg caggcgcgcg
      841 gagacaccgg cctcggtgaa aagcgcgcgc cccagctcgg cgagagaggg ttcggtgatt
      901 gtcaggtctg tgggactgtc atcaggcatg gacggacggg catggctgca atcaatctat
      961 gggaagacgc ggacgcggtt acagtgcggc ggcgctttct tcgttgcaaa agattcttgc
     1021 ctgagcggca gtctgtttgc tatctcaatc catctctgat gagcgagccg catcctaaca
     1081 gccatgatct attccccacg acacagtgga gcgtggtgct ggcggccagg gcggacgggc
     1141 agagcgggcg tctggcactg gacgagctgt gccggaagta ctggcggccg gtgcggggct
     1201 ttttcggcca gtgcggtgtg gcggacgatg agacggagga tctggcgcag gaatacttcg
     1261 ccagcctgct gacgcgggag tacctgcacc aggcggaccg ggagcggggc cgcttccgcg
     1321 cattcctggt gcaggatttg aagttctttc tctgggacgc gctggcgaaa aagcgggtgc
     1381 agaagcgcgg cggcggcagg gccttcgtgc cgctggacga gaccacggat gcggccgcgg
     1441 actccgctgg aaacacggcc ttctttgacc ggcagtgggc gctgcaaatc gtggagaacg
     1501 cgcgggagat gctgcgcgcg gagcatgagg ccgccggcaa gggggagctg ttccgcctgc
     1561 tgcagctggg cctggtgcgg acgatgagcg cggcggatta cgagcagtgg gcggcgcgcg
     1621 cggggatgac gcctggcgcg ctgaaggtgg cgctgcaccg gctgcgcggg cgcttccgcc
     1681 gggcgctgga ggagcaggtg cggcagacgg tggcgacgga gcaggacttc gatgatgaga
     1741 tgcgccacct gcgccgtgcg ctgcgggacg gattccagga tgctccgggg gatgaggcat
     1801 gaagcccgcg ccgcgccagg cgcttgagcg ggggaggcgg ctcagtgata gacttcggcc
     1861 agcgcgcgca ggagcttttc cagttcggtg aagtaggcgt cgtcgctgag ggtggggcgc
     1921 ttgtctttgt gggcctccaa ctgttgctcc agggtgtcgc gcgcggcgcg ctgggcgtcg
     1981 gtgaggcggg cctcctcgtc gctgaggacg aggacgagtt gcgcggcgcg ggcgccgtcg
     2041 ggtctggcgc cttcgggagc ggatttgggg cgggtgcctt cgaagacttc ggagcgggtg
     2101 ccggtgccgt cgccgttgtc ctcaatgaga gcgtgctcgg tggcgagccg gtcctcgttt
     2161 tgataaaact cggccacgtt tttgcaggca tgcaggaagg cctccagcac ggagacctgg
     2221 cggtcctggt cgaggtcggc ctcgggcagt ccggcgatgg cgggggcgaa gtgctcgccg
     2281 aagcgggcgt aaaagacttc gtcgccgcct ttggtggcgg tgacgatggt gcggcgcggg
     2341 ccggccaggg gcttcaaaaa cgcgccgctg gcggagccgg tgtggatgag cacgagcttg
     2401 cgcttcaggg gcgcaagcca ttgcgcgagc agggcgggag tgaggtccgg gccgcgcagg
     2461 ttgaacttgg cctcgcgccc gtcgtgggtg ccgtggccgc agagcacgag ccagagctgg
     2521 ccggcggcgg gttgcgcggc ttttttcaag gcggattcga cttcggccag gtcggcgtcc
     2581 ttgccgccgc tggtctggcc gatgagagtg aaggccgcgc cggcttttgc caccgcctcc
     2641 cgccacgcgt cggcctgctt ttggaaaatc ccggcgtact catcctcgcc cgccgcgccc
     2701 tgaatgaaga tgacctcaag gtcacgcgag tgcagggggg cggctgcgat gatgagccac
     2761 agcggggcga tgatcccgat tccccgccca agcagcgcgc gcgcgaggct catgccatgc
     2821 ctcctttccg gcgcagcagc cactcggcgc cgagcagtgc gaggatgagg gcgaagaacc
     2881 agggggtgtg ccagagcggc tcgctgagtg tctcgcgcac gggcacttcg atggcgctga
     2941 gcagctcggg cagcttgtcg aggtcattga gggtgagcaa ccgtccgcca gtctcatcgg
     3001 caaggcgctg caaagtcgcg cgcgccggct ccagcctcga catttcctcc acctgcggct
     3061 ggaagaccca gccggtgctt ttgctggaag caaagggggt gtgctccacg ctttcatcgg
     3121 gaggcgcggc attttccact gtggcggtga cacgataggc gcccgctgtt ttggggaaat
     3181 actcggcctc gaagagtccc ggctcggcca ggctcggctc gccatagagc agggacttgc
     3241 caccctcggg gccggtgacc tccaacttca ccaacgcgtc atccatgggc aggaagtccg
     3301 cggcgcgcgc ccgcaccgcg agcctcacac tctgctccag aggctcggcg gccacctgca
     3361 cgcggtcggg cacgtccacc accagccagc gcatgagctg acgccagaag cgctcctgat
     3421 ccttcatggc atccgcgtcg cccatgcccc agcgccagac atctccgaga gtgagggcca
     3481 ccacgcggcc ctcgccgaaa cgctgcgcgg ccagcgcggg gtaagtggcg tcctgggtgg
     3541 agacggtggc cagcacgctg gcgcccggct tgatggaaaa ggtctgattc accgagtgga
     3601 aaccgggcat gcctgccagc cggccctcgt cctcctcctg gctggggcgc aggcgcgtcc
     3661 acggctccag ccagccctcg cgggtgagat tgaagcgcgc gttttgcacc ggcggcgcgc
     3721 tggtgacgcg gtccagatac acgggcagca tgcggcccac gggcgtgtgc tcatagccgc
     3781 ctgcctggta gctttcctgg ccgccgagca tgagcagcgc tccgccgcgc tcgctgacgt
     3841 ggcgctcgat gagcttcatc tgctcctggg tgaaaaaatc cgcctccagg tcgtcgagga
     3901 tgatggcccg gtattcgcca aagagatcgc cggcgctttt cggaaacccc tctgaaagct
     3961 cctgcgcgtc cctggtgccg aggcggatga gcacgggctg gtcgtaacgc tgcgcggtgc
     4021 tctggtcgcc gaagccgcgg aagagcgggt tgctgctctc gcccacgcgg ccccgccatt
     4081 cgaacttggg ctcgcgcttg gcgatgcgga tgagggacgg cacctgcacc tcgtcgtccc
     4141 ccgccagcgc gcggcggatg aatttgtact cccagttggg ccggcccgag acgtagagca
     4201 cgcggtaggg tccctggccg cggtccacgc agacgaggcg ctcgttgttg gacagcaccg
     4261 cctctttggc gagcgcgcgc cactcatccc tggcggcttt cgccagcagc gcggactcca
     4321 tcaccgcgat gcgcaggaaa gacacgcccg gtttgaccac gggcactttc agcctggcca
     4381 cggcctgcgg cggtttggcc aggactttga gtttttccga gaccagcaca cggccctcct
     4441 catccagcgc gcaaaccgcc gcctccctgc cgtcgaatcc gcgcgcgtcc cacttcaccg
     4501 tcatcatcac cggggcgtcc tcaaaggcgg tttgagtcac gatcacatcc tggatggcca
     4561 gatcgggcgc gggagcctgg tcgccgacga tgaccggaaa cacgggccgc gcgccttcgc
     4621 cctgcgtcca gccgggtgag gtggcgtccg tggcatgacc atcggtgaca agaaccacgg
     4681 cggccaggga gcgcccggcc gccccttcat ggagcatctt cagcgcgcgg gtgagatcgg
     4741 agcggcggcc gctgaagtcc agcgcgccaa agtcggcgac cccgcgcaag cgctcgtcgg
     4801 cgagcagggt cttgacgcgg aagatgccgg agagcgcgcc catccaggcg gactcgccgt
     4861 ccgcgccacc gccgagggct tctttgacgg actgggcgcg ggttttgccg ccgcgcgtct
     4921 ccgccacggt gagggaggcg ctgttgtcgg cgacgatggc aagctcgttc tcacctttct
     4981 tcggctgctg gcgcgtccac aggggatcgc tgacgcatag cgccaggagg gacaaggcgg
     5041 ccagcttgca caaaaacgca gcccattttc tccagccgcg caaaggcgag cgcaggtaac
     5101 ccgccagcag gactccaaaa cccagaacca tcaccgcgca ggtgatccat agacgttcgg
     5161 gatgttgcaa aaggaagttc aagcgccgcg gaaaaagccc tccagccatg acgaacggag
     5221 ggcggcctta gataacaccg aaacggggcg atgttttcag aggaatccga agccggaaga
     5281 atgccatgcg gcaaaaagat gcgcaggcca gacctggaag cctccatcga gcggcttccc
     5341 gcgccagtgc ggctgcggcc atgggaaaag gaaaagaagg acgctgctta gctgcgaccc
     5401 cgccgcaacc tggaggactc agcagcgcca gcacgaagcc gcgctcgacg gcctcgttga
     5461 tggccgtgcc gatgacatct tgaatgggct gcgccgatgc gccggaatcc tcagggctgg
     5521 tcaccgtgaa gtcatgccgc ctcagcaccc ggcgaagggc gtcggcggtc gcgcgatccg
     5581 ccgaagcgca ggagaggaag acggtcgcgc gcttcgagag gcggatgact tggtggattt
     5641 ccgtctcaaa gtcgcggcgt gaatgggaga cgaaatccac atgccatgcc gacacgttgg
     5701 cgattcgccc tgcgggccaa aggcccatcc acaagccagc ccagggcaac gccctgggtc
     5761 acggacaccc aaagaacatc aagccccaac ggggcggccc aaatcccggt tcacgtcacg
     5821 aacgccccgt gtgcaaatcg tattggtgat gtgcaagtgg gttgtgccgc cctttcaggg
     5881 cttgaacaat ttgtggggca cgaaacccag ggcgttggcc tgggctggct tgtgggcgca
     5941 ccgttggtgc tcgaaaaatc gcggtcgagg tcgatggtct cgaccacctt gccctcccgc
     6001 gccagggatt tgaccagctc catctcgcgc aacacggcgg cggagttccg cgcgttgggg
     6061 ctgtcgcaga ggatgaacca ctcgcgcgcc ttgatttccc gttcgatcaa cagccacaac
     6121 agccggtcgt ccgcttccgt ggtttccatg cacttgagga agaacagcac gggattgtgc
     6181 ccgcgccggt ccaattcgtt ccgaatctcg cgaaccgtct ctaagtcgag gtgcgagttg
     6241 gggacgaatg cctaatacga atcggcttac caaggctgga gcttgcctcc gttgaccggc
     6301 tggctgagct tgaaatttat ccaactggtg catgaggccc agcctaaaac tagaaagatg
     6361 gcttcccacc cgatcgacag cggtgcagca agtgggaact ggtaggcgag aaccaaagcc
     6421 cagctcagcg ggttaaggtg tttgagcaga aagcggatgc ggctgatgtg gctggctcgg
     6481 aagtccctga actgagcacc tgccaagata tttgccgtga ggttcatgag ttcccgtccg
     6541 ctgaagccta ggaggaaggc acagccaagc agtttccaca gtgggagagc caggagatct
     6601 ccgtagccgt gatgccattg caactgcagt cccgcgaaga tggggccgta atagaccatt
     6661 agtcggctga gtgcggtgat cggggaatta agtaggccaa gaagaaagga cagaaacttg
     6721 gggaagatag agacggtgtt cccaaaccgg tgcttgtgga aagcttcctc aaagatgacc
     6781 ggcgtgaggg aactggcttg agtgatacgg actccaccca agccttgcgt ttgcggagaa
     6841 ctcaggagga gatcaaaggc gggtttcata atacctgagc cgacggacgg aagcgggctg
     6901 tccggcttga gcacgaggac gatctgtggg gcgcaccgct gaaaccaagc gtagagggcg
     6961 gcttcggttt ccgagttagt ccactcccgc tgaactgtga tggaatccac gagagagata
     7021 aagacgcggc tgcggttgat ggcccgcagg agatggcggc gccagttgcc acccgcttcc
     7081 aagctgagtg tgtccaggta aacctcgcca ccggcctcac gcaggcgctc gtaaagcgtg
     7141 tgcgccaagt tgccccccca ttcagaagct cggctgtagc tgatgaagat agcgcgatcc
     7201 agtggagcaa agagtgggca gcgccaaggg cttgggggaa gcccttggat ggtccaccat
     7261 tgccacttcc gcagtcgggt gagcacgttc cgggtgagaa gccgggtagg agatagggag
     7321 acggggcgct gacgacgcca actttccaga atcaagccgg agacgaagcc caaggagaac
     7381 caactcgtgg gtgggtccag cacacggccc actcactgcc caggaagcca gatcgcaagg
     7441 aacaaaaaca tccagacgaa gagccggttt ccgccgaagg cagcggggga aggcagccaa
     7501 ctgtagctta cgccgctacc cttcagtgtg cggctaaaag ctcgacccgc gacgtagaaa
     7561 acgggcatct gaaaaacaca gacgttcagc cccatcatcc agcacagaag ctcggaataa
     7621 ggaaccacga gaggtcgtgt ctgctcttgg cgaatgagaa gagctgcggc ggttaggatg
     7681 accaaaatgg gtgctatagt gacaataaga tgagccaagc cggcgaacag ccaggcccat
     7741 gcgttcgccc aagtgcgcca gcggttctcc cagcgggcct ggcgaatact agaggtgtag
     7801 caattgatgg cttggagcaa ctcggccttg gattgaccgg gcactaggtg aatgttctcc
     7861 agaatctgat tggccagtgg ctcgccagca gccgactgtt gcttgagcca ctcaatctcc
     7921 gcttctgagg ccgcgatgaa cgcacggaag atgtcctgct gcttcataag cttaacgatg
     7981 ccttggatgg cggtttggaa gccggggagc gagaactggc tacgctggaa gacgacaagg
     8041 acagccgttg agcgacggat gacgcggcgt atctgattcg agtcggcagc cgtcaggtcg
     8101 cacgtctcgg tgtgaggtcg gcgtccgcgc tcgcgagaaa tgccgagcgc gaaaaataga
     8161 taccccagca ggatggagat tggattgctc cgcgccttgg cgtccaccat cgcaccgcct
     8221 tcttccgcag cttgggccat cgccgcaaga gccgtcggct ccgtccccag aatggcaatg
     8281 ttacgggaac tgggcgaagt gccccagatc cgggaacttc gatcctctgc cggggctggc
     8341 gaagaagggg caaccgcgac cggcggagta tcacggaaca cggcgtccaa cgtcttcgcc
     8401 atggggtggt ttggggccac ccgttccaac tgcttcatgg aggcaatagc gtccgatacg
     8461 ttgtatagaa tggtatcgat ccgcacgcag cgttcgaagc aggagattgc ttccccagtg
     8521 cgcccctgga cacgggcgat ctgacccaag taaaatcgga cagcgctttc ctctgcaaga
     8581 tcgctgcgat ccaattccgg gagggaatcg aggatatttt gggcagtcgt cagctcttgg
     8641 agtgcttccg gtaatcggtt ttgcgcccag tagatctggc ccagcttgcg gcgggcaaat
     8701 gcttggtccc ggtaagcgct ttccgcctga cagagcaaga tggcttgatg gtaggcttgg
     8761 tgagcctgct ggaggcgttc atcctgacgg gcgagttccg ccgaggcgga gtccgggccg
     8821 gtggcgggaa cggggtgctc tgcgtaaaat gcttcctgca aggcaactcc ttgccggtaa
     8881 tggtcaaatc gagtcatggc gatttctaat ttgtgggtgc attgaaccgg acagcgagag
     8941 agccagcggg ttggcgagga ggtagtcgaa gagttccgcg gggtctttgc ggtcgagttc
     9001 ggcaatctcc ggcagggtga gcaggagctt gcggaagtcg ggcaggcggg tggcgagctg
     9061 gaaggcgagg ctgcggatga cgcgccgggg gtcgcggtgg tcgggcttgt cccattcgac
     9121 gaactgcgcg gcgatgacgg cgtcggagcg ggtgtgggtg aactgggcgg caaaggcgct
     9181 cttgcccacg ccggggtcgc cggtgatcca gaagaggcgg ctgggagttt cgaatgacga
     9241 atttcgaatg tcgaatgggg acgagccggt ctggggaccg gcgttcccgg ggttgcgcca
     9301 ggtttcgatg gcgtcgaaga tccattggcg gccgaagaag ccttttttga ggaggtcgta
     9361 gatgcgggcg tcggagcgga taggcttgag gtgggtggcg agggtttcga tctcgcctgc
     9421 aaagcggcgg ctttcgtcgc tctccacgac gcggacgatt tcggcgagtt taccctgata
     9481 ccacgtttcc caagaatcca gattttcagg cgagacgccc gatccacgtt cctgccgttc
     9541 tttccagtca tgcatgtcga gccactggac atggccgatg ttcaccggcg gctgcacctc
     9601 ggcttcgctt tcgacgagga tggtctggat gttcccgcct ttcacaccga gggcgatggc
     9661 gatttcatcg cggcagacgc cggggtcgcg ggtggagtgc ttcgagagga acgcgacgaa
     9721 cttctggctg ccggtgatgc cgtcggtgat ggagcgccgc cagtggtcgc cggctttgat
     9781 ttcgttttga tcgaaccaca cgtcatgccc gcgctcttcc agatctgcct tgatgcggcg
     9841 gacgagttcc tcgttggcgt cgtggccgta gctgaggaag atgcgcagcg gggtggcgac
     9901 cttgtccggg gtgaactcgc gcctgcagtc gtagcacaag aatgctgccg attcaggaat
     9961 cggcatcgtt ttggcggact ggcagtgagg gctattcatg cctgaagaaa ttcgccagca
    10021 ttttgcgatg ccggagagtc gcttgtcagc agtttttttg caaacgaccg ggattcctca
    10081 gactggacac cagacacccg catccatgat gcgggcgcaa gatcagatgc cagccgtgga
    10141 gacttcccgg acggcgcggg agaggatttc ctcctggagg gcggccatcg ccgcggcttc
    10201 ggcggggtcg tgatcgtccc agccggcgag gtgaagcagg ccgtggatgg cgtagagcgc
    10261 ggtttcgtga tcgaggctct tgccgtgggc ggcggcctgg cgcagggcgg tgtccgcgct
    10321 gatgaggatc tcgccgtggt ggaaggtgat gacgtcagtg gcggaggggt cgccaagaaa
    10381 ctcggcgtgg acgcggctga tctcggcgtc gctgatgagg gtgatctcga tctcctccag
    10441 gcctgtgagc ggcgctccgg aggatttgac ggcggcaatg catgcgggca tggcggcttt
    10501 gaccacgcgg cgcagccagg gcaggggcgg gcgcggagag cgctgctggt cgctgagcgt
    10561 gatgcggggc gtcgtcctca agcggcggag gcgggcggct tgcgcgctga gcgctttttg
    10621 aattcacccg tggcggtgtc cccgccggcg ccgggcctgt ccatgacaat ggtgaggccg
    10681 tcgtgtactt cgggcttggg cgcggctggc gggatgccgg actcggcggt cttttgatac
    10741 ttgatgcggc tgtgcatcat gctgaggagg gtcttgacga aactggcctt gaccctggcg
    10801 aggtcggaga tggtgaggtc gcactcgtca agctggccgt ccttcatgcg gtcgagcacg
    10861 agctcgtcca cgagggtctc aatgcgggag gcgttgggct tttccatggc gcgggaggcg
    10921 ctctcgacgg cgtcggccag ggagatgatg gcggattcct tgaactgggg cacgggaccg
    10981 ggatagcgga agacctcggg gctgaccttg ggcacgtcgt cctccttggc cttgccctcg
    11041 ccgacgagac gctgcacttc ggcctgctgc tggagggcgc ggttgtaaaa gtactgggtc
    11101 atggaggtgc cgtggtgctg ctcgatgacg tcaatgacgc tgcaattgag cttgtgcttg
    11161 atggcgaggt ccacgccgtc cttcacatgg gcgatgagga tgagggcgct catgcgggcg
    11221 gtgaggtcgt catgcgggtt gtccgccggg tccatgtttt cgatgaaata ctcgggcttg
    11281 gcgagcttgc cgatgtcatg aaaataggcg cagacgcggg acatggcggc attggccccg
    11341 atggcctcgg cggcggcctc ggagaggttg gcaacgacga ggctgtgatg ataagtgccc
    11401 ggcgcctcca tgctgagcct tttcatcagc gggtggttca agtcggcgag ttcgagccag
    11461 gaaatctcag tggtgacgcc gaagaggctc tcgatgaccg gcagcagacc gccaacgagc
    11521 agacccgtga tcacccccac agcgaaggga acaccgagca cctgcgcggc cagggtgctg
    11581 tccagcggcg tatcggtggt catgtgcagg caaagggaga acaaggcggc caacgcgccc
    11641 gcataaagac cggaggcgaa caggcggttt ctccgccgca cacggcgggt gaaaagcacg
    11701 accaggaatc cgatgagaac cgaggcggcc agataaatga gcggattgac ggccacacag
    11761 acgagggtgc ccagcagcgt gccgtagatg gcgccgaaaa gaccgatcct gcgccccaag
    11821 gtcaccgaga gaaccagcgg tgcgaaggca tggggcagcg ccatgaccag cagggcgccg
    11881 gtccagcctt cacgatggcc gaatttgagc atcaggacat gccccgcgaa ctgcaaaagc
    11941 aaggtgccta acaccagcag cagttcggca ttgtcctcca catcctcaat aagggacacg
    12001 cgcagatgca cgaccatggc cgtgaccagg gcgaccgcca gcaggtaggc cagcggctgg
    12061 ggcgccaccg tgccgggaat gactgcggcg cccgcctgca tgagcacgcc cgtgatgagg
    12121 aagaagccca aaaacagaag gaaagtgacc acgggttgag tgcgcagctc gtcaagaagg
    12181 tcgccttcct ggtgtttgcg gcgcaccctg ccgcaggaaa ggccttcccg ctgcaatttg
    12241 gcccggcgta tggattcaaa catggatgtg atttgcggga gctctgaggg atcatgtagc
    12301 acatcccccc tgccggacaa gcctggattt ggcccgggaa gtgttcatgg agacagttga
    12361 aaccgggcga gtcccctgcc ccgccatgcc tacccggaca atcttccgca tccgtgcttg
    12421 gcaaaccaag ccgccgcgcg caaactgtcg caagcccccg cgaactctca acccgacatc
    12481 cgaccatgcc acgcaaactc agccacatcg ctcccgactg gtgggactac accactcttg
    12541 atcctgaatt gatcaatgat gccgcccggc tcaccgagcg cgacctccgc cagctctccc
    12601 gtcccggctt caaagtggtc atgtacgact ccctggagga cttttacctg gccgaggctc
    12661 tggagtacat ccatgcctgg aaacaggcca ccccggacaa tccggccggc atttgcggcc
    12721 ccatcggccc caccgagcag ctcccgctcg tggcgcgcct ggtcaacgac ctcggcatct
    12781 ccatcaaaga cgggcacttc tggggcatgg acgagtggta tgacccggag acgaaaaagg
    12841 aggtctccat ggaccacccc ctttcctttg aaaaagccga ccgcgagttg tgcttcaacc
    12901 gcattcagaa gaagctggtc atgcctgatt cccagcttca tttccccaaa gccgatgtca
    12961 ccgcctattt gaaaagctgg cagagcgggg tgcgctgcgt ggtcatgcag ggaggacagg
    13021 gcgacatcaa gcactgggcc ttcaacgatc ccctcaagcg tgccggcaag tggaagaacg
    13081 agccgccccc gcccgccgaa taccgcaaac tgggcacccg aatcgtcgaa ctgcaccccg
    13141 tcacactttc ccaaaacgcc cgcacctcgg gtggcggcaa catcacgatg gtgccgcaga
    13201 tggccatcac ggtcggcccg aaggaaacct ggatggccga gaaagtcagc atctggcagg
    13261 ccggcatgca cgacaacccg ctcggccagc gcctcaccgc gctgatgatc tccaagcgcc
    13321 tcgccgactc gtccgtgccc atgtccctgc tggcggacca ccagaacgtg cagttcaact
    13381 actaccgcgg cggactcgga agctgcgcgg tggagatgca ctgagcctcc ccccccggca
    13441 aaaaatgcgg gccgctttcg cggcccgttt ctttttctca tgaggaggcg gtcacatcac
    13501 cggccgagcc gggatccgca tcgtccgtcg cggcggcatc aactccgtca ggatcaccag
    13561 cccctgtgtc cgcaaccgcg ctttcatcct catcctccga agccaccagg gcaacgtcct
    13621 gaatggtttc gccttcgttg agagtcatca gcttgacccc ctgggcgttg cggccggttt
    13681 cgcggatttc actcaccttg atgcggacac tctgcccttt gctggtcatc agcatgagtt
    13741 catcagactc atgcacagcc agggcggcga cgactttgcc ggtcttttcg gtgacgttca
    13801 tggtgatgac acctttgccg ccacggttga tcatgcggta ttcctggaaa ggcgttcgct
    13861 tgccgaggcc gttctcgctg acaaccagca ccatcgtttc cggctcgttg gtgaggcagc
    13921 tcacgaggaa gtcgtcggca tcgagcatca caccgcgcac accagccgtg ttgcggccga
    13981 tcggacggag attcggcgag gcgggatcgt cgccggtttc gtgaaagcgg acgcacatgc
    14041 cctcatgcgt gacgaggcag acttccttcg tgccgtcggt gagcacgaca tcgacgaggt
    14101 cgttgccttc atcgagcttg atggcgatga tgccgtcctt gcggtagttt ttgaactctt
    14161 cgagagcggt tttcttgatg gtgccgtcct tcgtggcgaa gagcacgtag cgatccgcgg
    14221 cccacatggc ctcatcctcg gctccttggg ccttcaagat gagcacggag gcgattttct
    14281 cttcgttgcg gagattgagc acgtttttga tgctccgacc tttccctgtg cgcgtggcct
    14341 gcgggatctg atacacgcgc tcgacataca tgcggccggt gttcgtgaag aagagcagga
    14401 agtcgtgaag ctgggcgctg aagagatgct cgacgaagtc gtcggcgtcc tcctcgctct
    14461 gcgcctcgcg ggtttccatg cctttgaggc cttttccgcc gcgtccttgc agtcgatact
    14521 cggccgtggg cgtgcgtttg atgctgccca tgtgcgtcat cgtcacgatg gcggcctcgt
    14581 tcggaatgag gtccacgctt tcgatctcgc cggagacggc ctcgatgcgt gtgaggcgcg
    14641 gcgtggcgtg cttggccttg atgacgtgaa gctcgtcttt gatgatcgtg agcacgcggt
    14701 gctcgttcgc gaggatgtcg agcaggtctt tgatggtggc gaggatggcg tcgtattcgg
    14761 acttcacctt gtcgcgctcc atgccggtga gctggtagag gcgcagttcg aggatgtcat
    14821 cgacctgctt gtcggtgaag acgtagcggc cgtcctggat gctcggctgg ctgcgaatga
    14881 ggatgccgag ctgctcggcg gtggcgacgg cgaaggtgta ggccttcaat ccggcacggg
    14941 cctcgtcgcg gttgcggctg tcgcggatga ttttgatgaa atcatcgaga tgaccgagcg
    15001 cgaggaggaa ggcttcgagt ttttcagcct gcacctcggc cttgccgagc aggaacttcg
    15061 tgcggcggat gatgacctcg cggcgatgct cgatgtagca accgatggcg tccttgatgc
    15121 caaacacacg cgggcggttg ccgtcgatgg cgagcatgtg gacgctgaag gatgcctcca
    15181 gctccgtgtg cttatacaaa ttgttcagca ccacctgtgc acgcgcatcc ttcttcaaat
    15241 cgacgacgag ccgcgagttc tcatcggact cgttgcgcac gccgctgatc tccggaatga
    15301 ttttttcgtt cgccagctcc gcgatcttct tttccagctc cgcgcggttc acgttgtagg
    15361 ggatttccgt gatgatgatc tgctcgcggt tgccattttc gacgatctcg gcctgcccgc
    15421 gaatgcgcac gctgccgtgg ccggtttcca tgtattcgcg aatgccgctg gtgcccatga
    15481 tggtgcagcc cgtcgggaaa tccggccctt tgacgtgcgc catcagctcc ggcgtggtga
    15541 tcgcgggatt atcaatctgc gcacaaaccg catcgacgac ttcaccgaga ttatgcggcg
    15601 gcatattcgt cgccataccg accgcgatgc ctgtgccgcc attcacgagc agattcggaa
    15661 acgccgccgg gaacacggtg ggctcggtga gacgttcgtc gtagttcggg acgaaatcga
    15721 ccgtgtcctt gtccatgtcc tgcatcaagg caccgccgag atgcgtcatg cgcgcctccg
    15781 tataacgcat cgccgccgga ggatcgcctt ccacgctgcc gaagttcccc tgcggatcga
    15841 tcaaagtctc ccgcatggcc cattcctgcc ccatgtgaac gagtgtcgga taaatcaccg
    15901 cttcaccatg cggatggtag ttacccgaag tgtcacccgc gatcttcgcg cacttcacgt
    15961 gcttcttcgg tgggtacagc gacagctcat gcatcgcgta aaggatgcgc cgctgagagg
    16021 gcttcaatcc gtcgcgcaca tccggcaaag cgcgggagat gatgacggac atcgagtagt
    16081 cgaggaagct gttcttcact tcgtcggcga ccgagatggg gcgtgtgttg gagtcttgca
    16141 tcaatcgaat cggaaaaggg ttgagtcagg cgatggcttg atggcgatca cacgtcgagg
    16201 ttgcggacgt tcagggcatt ctcctcgatg aagtgctttc tcggctccac gacatcgccc
    16261 atgagaatgg tgaagatgcg ttcggcttca tgggcgttgg attcgtcgag cttgatttgg
    16321 aggagggtgc gcttcgtggg gtccatggtg gtctcgaaga gctgtttggc gttcatttca
    16381 ccgagacctt tgaagcgctt gatctccatg ccgcgtttgc cgatctccat gaccttgtcg
    16441 aggatgccgg ggatactgaa tacgggatgc accttggcgt tgtcgccatc gccttcgatg
    16501 acttcaaaga gcggcttgtc ctggctgctg aagtgatcga cgtgcagacc gagctcgtcg
    16561 agctgcttga agcgggcttt catgccgtgg gcttcgtgga cttcgatcaa gcgggcgcga
    16621 cgagtctgct tcggtgcggc cgttccgttg gcaccttcga catcgggttt gccaaagaga
    16681 tgcaggtcgg cgttttcgcg ggcaaaattg gccagcgtgt cgcggtcgag caggtattta
    16741 acgttcactt cgttgccctc gcggatgatc acgaggtagt gaggcagttc gccggtctcc
    16801 gtgttgcgat gctggaggta ggtctcgaaa tccgcgccgt ggcggcggat gatgtcggcg
    16861 aatttcgcca tcggaacgag caggccgagg atttgcttca acttgtcgga atcgaccttc
    16921 gtgttgtcgg acacattgcg cagcgtgatt tcaccggagc cgagttcgag gaggatggcg
    16981 tccatttcct cgtcgttctg cacatactcc tcgcgttttt tgcgcgtcac ctgatacagc
    17041 ggcggctggg cgacataaat gtggccgcgc tttaccagtt cgggcatctg gcggaaaaag
    17101 aaggtcaaaa gcagcgtgcg gatgtggctg ccgtccacgt cggcatcggt catgatgatg
    17161 attttgtggt agcggagctt ttcgatgttg aagctgccct ccacctcgct gtcgccgccg
    17221 atgccggtgc cgatggcggt aatcatggtt tggatttcct tgttcgcgag caccttctcc
    17281 aggcgtgcct tctcggtatt gatgagcttt ccacgcagag gcaaaatcgc ctgattgtga
    17341 cggttgcggc ccattttggc ggagccaccg gcggagtcac cctcgacaat gaagagctcg
    17401 gtcttcgccg cgtcgcgttc ggagcaatcc gcgagcttgc cgggcaggcc gccgctgctc
    17461 atcgcgtcct tgcggatcgc ctcgcgggca cgagccgccg cgagacgtga tttggccgcg
    17521 atgatgatgt tatcgacgat ggccttcgcg acatccgggt tgtgatcgaa gtgatgcaac
    17581 actccctcat agacgacgga actcaccacg ccctcgacct cgccgttcac gagcttcacc
    17641 ttcgtctgcg agttaaagcg cggatgcgga tgtttcacgc tgagcacgca ggtgaggcct
    17701 tcgcggcagt cgtcgctgtc gatgtcgagt tcaggccact tcgccttgtc tttgatcggc
    17761 gccttggtct tgcccttctc gccatactgc tcgggatggt cggtgatgta ttttttcacc
    17821 gcacgagtca gcgcggtctt gaggccggta taatgcgtgc cgtagtccgg attcgggatc
    17881 gcattggcaa agcagagcgt gtgctcgccg aggcttttgt gatactgcat gacgcagtcc
    17941 acgaggatga acttgttttt gtcctccagc accacctcgc ggcgtccggc gatgacgatg
    18001 ggctcgggat gcaccttgtc tttgccctcg cctagttcgt tcacgaactg cttcacgcct
    18061 tcggtgtaga gcagcgtctc cgagcgagcc gattcactgc gttcgtccgt gagatggatg
    18121 gtgatgccgg gatttaggaa tgccagctcg cggagacgtg tgcgcaggcg ctcaaaaaca
    18181 aacgtcgtcg tgatggtgaa gatggtcgca tccgggaaaa agctgatctt cgtgccagtg
    18241 cgcttcggat catcgagatc accgagcacc ttcagcggct cggtggtgat gccgcgctca
    18301 aagccgatgg cgtggatttt accatcacga taaacctcgc acttgaacca gtccgagagc
    18361 gcattgacgc acttcgcgcc gacgccgtgc aggccgccgg agaattcata cgctccatcg
    18421 ccgaacttgc cgcccgcgtg caggttggtg agcactaact cgactgtggg catccctttg
    18481 gcgtgcattt ccaccgggat gccgcgtcca tcgtcctcga tgctgatgga gccgtcactg
    18541 tgaatgctga tccagacgtt tttgcaaaag cccgcgaggt gctcatcaat cgagttgtcc
    18601 accacttcaa acacgcagtg atgcaggccg cgctcatccg tctcgccgat atacatgccg
    18661 gggcgcatgc gcacagcttt gagcccttcc aggtgctgga tttgttcggc gccgtaggcc
    18721 tggtcagcgg caacggcggt ggagtttgat cccgtggaat caatgatgtc gtctggcata
    18781 aaaaagtcgg agaaaccacc acctgaattc gtccgtgaaa tcccttcgga tcatgcaccc
    18841 ggtggaggtg atttcagact agcaagcccc ccctccatgc caaggtaatt gtgccaaaaa
    18901 atcaggattt tggatgaaag aaccatgaac tcctgacagc atgccggatg acatccatgc
    18961 cccttgaaaa cccctttgat ttcatcgtac tcggcggcgg cagcgcgggc tacgcggcgg
    19021 cgcgcacggc gcattccctg ggcatgtcgg tggcggtggt ggatggagcg gcggaactgg
    19081 gcgggctgtg cattttgcgc gggtgcatgc cgagcaagac gctcattgag tccgccgacc
    19141 gaaatttgac tcttcgccgc gcggcggagt ttggtctggt ggcgagtccg ggcgcgccgg
    19201 acatccgtgc gatccgggac aggaagcgcg cgctcatcgc ggacttcgcg ggataccggc
    19261 gggggcagct tgaagacgga cgtttcaccc tgctgcgggg acgcggagtc tttaaagacg
    19321 cccggacgct ggtggtgagt ccgctggatg gcgcgccgac tttcgaggtc gcgggacgga
    19381 gcttttgcat cgccaccggc tcggtggtga atgtgccgga tgtgcccggg ctgcgcgagg
    19441 cgggtttttg gacgagtgat gatgtgctcg acgcggaaga gctgcccggc tcgttcctcg
    19501 ttttgggcgg gggcgcgatc gcgctggaga tggcgcatta tctggagggc atggaccggc
    19561 gggtgaccgt gctccaacgc gggccggggt ttctttccgg actggacccg gaatgcgggg
    19621 agactgtgag gaaggcgtat gaggcgcgcg gcatcacctg ccgtctgggc gcgcggctgc
    19681 tgcgcgtgga gcgcgctgcg gaaggggcaa agcgggtggt ctttgagcag ggcgggcatg
    19741 aactgggcgc ggcggcggag gaggtgctcg tggcgctggg ccggagtccg gctgttgagg
    19801 ggctgaggct ggaggcggcg ggggtggctt tgcatggcaa aaagatcgct gtgaccggcg
    19861 cgatgcagac gagccagccg catatcttcg ccgccgggga tgtgtgcagc ccgctggatg
    19921 tggtgcatgt ggcgatccag cagggggagg ctgcggcgcg caatgcgcgg cgcgtgttgc
    19981 agggcgtgca ggggttggaa agcgtggact accgcttgct gctttttggc gttttttccc
    20041 atccgcaagt ggccgccgcg ggccaaagcg aggccgccct ggccagggcc ggcatcccat
    20101 tcgtgagcgc ctcatacccg tttgacgacc acggcaaatc catgtgcatg ggagagacgg
    20161 aggggtttgt gaaaatgctg gcgcaccggg agacgggaga aattctcggc gccagcgttg
    20221 ccggcccgca tgccacggag ttgattcacg aggtggtggc ggccatgcac caccgctgca
    20281 cggcgcatga gttcatgagc atcccgcatt accacccgac actttcggaa atctggacca
    20341 acccagccga ggagtgcgct gagctgctcg acccgtccgc agcgtgagcg cgtcgcggct
    20401 cacagggtca tcggccgcat ggcggcgcct ttttccaggc agggaggcat ggctttgcgg
    20461 aagtcctcca ggctcacagg cttggtgagg aaggcgttca tgcccacggc cttgcactcc
    20521 tcgcgcacac cggtgagggc gtggccggtc atggcgatga tggcaggctg gtttttgaga
    20581 tggaaattcc cgcgaatggc gcgcgcggcg tctatgcctc ccatgacggg catctgcagg
    20641 tccatgaaga tgatgtcgta gctgccgcgt ccaaccatct ccacggcctc ctggccgttg
    20701 ttggcgatgt ccacctgctc gtagcctagg cgctgcaaaa gcatgacggc gattttttga
    20761 ttcagcggct ggtcctcgac caggaggagg cgcgcgggat gctggctggc aaaagtctcc
    20821 ccccctgccc tggccgctgc ggcaatggcg cggttggtgt ccttggagat gacggcgtcg
    20881 tgcccggcgg cggcggcgcg gttcagcgaa ccggtcgccg gctgatcctc atgaaagacg
    20941 ccgcgcagcg cggcgggagc gacaggctcg gggacaggag gctggagcca cgcgggatcg
    21001 ttgccgggga aggcgggagg gagcggctgg gcattcatgg cggcggggtt gtgggcaatg
    21061 agttgagccg cgtgcatggc cggaacctgc gcggcgggcg ctgtggatgg ggatgacaag
    21121 ggggtggtcg gagcgggctg gggcatccgc gccagctccg cgagcacgcg cagcagctcg
    21181 cgatgcttgg cgggcttggc cagcttgcag aaacgcgcgg ggatgcgggg tgcgaactgg
    21241 tcgcgcagct tccagcgggt gagcggaagc agcacgagca cgcccgcgcg gcggccagcg
    21301 gcggcctgga tcagcggcgc ggcggtttcg ggatcatggg agctggcgtc gagcatgaaa
    21361 aggctctttt catccaggtc cgccgccact tcccgcgcgg aggcggcggg cgcatgaatg
    21421 ggaatgacac cccattcctg aaagtgctgc tgaagaatct ggcgggtggt ttgatgcggg
    21481 gtgtgaaaga cgacgcgcaa gcctttgacg gcggtctccc atgccagctc ctgctcgcgc
    21541 cccgcctcgt ccgccgacac gcgcagcggc agctcgaaga agaagtcgga cccctgcccc
    21601 tcctcgctga tgacgctgat ctcgccgccc atgaggtggc agagcttgcg cgagatggcc
    21661 agtccgagac ccgtgccgcc atatttgcgg gtggtggagc tgtcggcctg cgtgaaggcc
    21721 tggaaaagcg tgccgatctt gtcggcgggg atgccggggc cggtgtcgcg cacggaaaaa
    21781 tgcaggtgcg gcatctcgcc attgcgggtc ttgcggctga catggcgggc gaggatgagc
    21841 acctcgccct tgtcggtgaa tttgacggcg ttgccgacga ggttgacgag cacctgcttg
    21901 acgcgctgga agtcgccgcg gaagaagcgc ggcagggccg ggtcggcgtg gaagttgagt
    21961 tccagcccct tttcggcggc gcggtaggaa aagacatccg tcacctcggc gagcagtttc
    22021 tccatttcca cgggcatgtc ctcgatctcc atcttggcgt tctcgatctt ggagaagtcg
    22081 aggatgtcgt tgatcaaatg cagcagggac acgccggagg agtggatcat gcgcacgagg
    22141 tcctcctgct cgggggccag acccatctcg atgagcaggg aggtggtgcc gatgatgcca
    22201 ttcatgggcg tgcggatctc gtggctcatg ttggcgagga agtccccctt ggcggcggtg
    22261 gccacctcgg cctgtttgcg cgcgcgctgg aactcggcga cgagatgctg ccgccgcgcc
    22321 tcctcctgct ggaaggtggc gatggcctgg ttgaactggt gctggagggt gtcgaggtcg
    22381 tcggagccgg tgacacgcag gcggtggtcc agatcccccc ggctgagggc ctcgaaaccc
    22441 tggcgcaggg cggtggtctt gcggctgatg gcgcggccca gaagatagaa accgagcacc
    22501 gccggcagca ggccggccac agccccgaag atgggcacca tgagacgcgg aagattgatg
    22561 atgtgacggc ggtggatgcg cggctggcgt gccagaatga cgccatcggc gacgccctgc
    22621 gggttgatga gcggcgcgcc ggcgatgacc cagccgccgg gaccgtcgag gtccgcgcgc
    22681 agggagagcg ggtcgtcgcc aaccactacg gatgagccgg ccagcacacg gaaaatgatg
    22741 tcgcgcgact gggcgaactc aggctccggc ggctgccatt cgagggctgt gtcgtggacc
    22801 aaaatgtagg gggaagtctc cacgggaggc tcgccattgc gggcgttggg cccggcgatt
    22861 tcaatgcgca gacccaggtc ctggatcatg ggcggcacgt ccacgacctg gaggcgcatt
    22921 ttcaaatgcg tgatggcctc ggaagtcaga tccttggtgc tggcgagcgc gcccggactc
    22981 acgccctggc tgcgcagctc ggcctgcgcg gcctggacga tggagccgag atacgcgcgc
    23041 tgggctgccc gcaggatcag catttcctgc cagaggaagg cggcggcggt gacagccgcc
    23101 gcggcgacca cggcgagcac gccgaagacc atgaaacgac cggatatgga aaaactcggc
    23161 ttcatggcgt gtcaggcgag ttgatgcgag cggacaaggc tgccgcgcac cgtggccacg
    23221 agatggtcca gcgtgtaagg ttttggcagg atgtcggaaa agccgatggc ctggcagaga
    23281 tcgcgcgcgt tttcctggaa atagccgctg cacgcgatga tgggcagggc ggggtccatt
    23341 ctgcgcatct cctccagcac ctcgaagccg gaaagccccc caggcatggt gagatccagg
    23401 atgaccagct cgatgcgctg gtcaggcggc gcgggctggc tgatcaagtt gagagccacg
    23461 tcgccggaat cggtgacgat gacctcaaat ccatgcgcgt ccaggatggc cttggcgacg
    23521 gcgaggacct gcggctcgtc atccacgacg aggacttttc cagtcgttcg atgggggtgg
    23581 ctgtgaatgt tcatggcggc ggcatggatg gcgggttgtc aggcggcggt gtaggcgagg
    23641 catttgatct catcgaggct ggtgtggccg gcgatgacct gcatgaggcc ctcctggtag
    23701 agggaaaaga agccgttctt gaaagcgcag gcgcgcatgg cggacatggg cgcgccctcc
    23761 tcgatgaggt cgcgcagctc ggtgctgatc tcattgagct ccatgagcgc cacacggccc
    23821 gcataacccg tgccgtggca ctcctggcaa ccgccggccg tgtagatggg ctgcaccagg
    23881 ggctggctga tgacgccctg gcgcgccatc atggcctgga tgtcgtccgg cacctgcatg
    23941 ggctgcttgc agtagctgca caacctgcgc acgagccgct gcgcctggga gagcgccagg
    24001 gagtccgcaa ggaggtattt ttccacgccc atgctcatga ggcgggagac ggcgcgcagg
    24061 gagtcgttcg cgtgcagcgt ggtgagcaca agatggccgg tgagcgcggc gttgacggcg
    24121 gcgttcgccg tctcggcatc gcgcgactca ccgatgagga tgatgtccgg gtcggcgcgc
    24181 agcagggcgc gcaggccgtt ggcgaagtcg aggccgtaat gcgggttggt ctgggtctgg
    24241 ttgatgccct cgatctcgta ttcgatgggg tcctcgatgg tctggatgtt gacgccgtcg
    24301 tcgttgacgc tgttgagcag ggcgtaaagc gtggtcgttt tacccgaacc tgtggggccg
    24361 gtgacgagca cgaggccctg gtcccgctgc atgacgcggg tgaggatgtc tatctggcgc
    24421 tggccgagat tgaagtcgga gaggcgcttc acgccgtcct gcttgtcgag gaagcgcatg
    24481 atgacctttt gaaactcccg gcgcgtgggc acggcggcga cgcggatgtc cacccgccgc
    24541 gcgccgacac tgacggcgaa gcgcccgtcc tggcactcct ggcggctctg attcatgccg
    24601 gcgtagtttt tcagcagcgc cacgaatcgg ttgagcatgg actcaggcgc ggtgaggatg
    24661 gtcttcatgt tcccgtccat gcgggcgcgg aaacgggcca gattgtaaaa cttctccagg
    24721 tggaggtcgg aggcgcggca gcggatggag gtgaagagcg cccactgcac cagctcctcg
    24781 gcgctgtggt tcgggctggc cggattgacg cgccccatct cgtccggcag gatgtcaatg
    24841 acggactcgt tgccggagac ggaggcagtg agcgtgctct tgtcgagctt tgccacgccg
    24901 ccggagtcaa aggtggcgcc ggcgcggttg atggcgtcgc ggatggcggc ggggtcggcc
    24961 tgcacgggca cgatcttcac cgcggcgttg cccatggaga gccactcgtc ctcgaaggcg
    25021 tagcagtccc cgctctcgga gaacatgaag accgcgtttt tgccgatgta gagcggatac
    25081 acggtgtgcc gctccagcag gggcagcggg aaaaaggtct gcctttgcgc ctccgccggc
    25141 gagaagcaaa gcgtgcgccc gccgctgacc aggtgctgca gcaccacgcc gaggtggcgc
    25201 tgcacggtgt tgaaatggga gacgcccaga ttggagtgct tttccttctg cgtctcgaaa
    25261 aagccgcgca gcttcgtcac ctccgccggc tcgtaggggt agctggccag cagccactcg
    25321 aaggcgccct ccagccccat gtccgcaaaa cgcggcgcct gcaccccctc catctggttt
    25381 tgctgggcga tggggagctg gccaaagcgc tgcaccaggt ccttgcgcgt tttttgatac
    25441 tggtcgggcg tgatgagcac gcgcagggtc aaaaacggcg gcacgcccca caggtcgccc
    25501 gcgcgcggat tgtggtgcgc catgaccagc agcggcccca tcgtggtcac gggcagccag
    25561 gggtcggcca cataaggcac ccggcccagc ttgcggacga tcttttccgc ctccggggag
    25621 cagctctcgc gcagcaggtt caccggcagc atgttgtact gcaaggccag cccttcgatg
    25681 gagaggatgg ccgcctgctt ggcgtcaatg gttgccgcag gtggaatttt cgactgtccg
    25741 aaattttcag acgatgccgg ggtggatgcg agaacgacgg cggacatggt gcgtgggcgg
    25801 tctcagcggc cgctggattg atagttttcc ggaaactgga aagcctcggt gaaatccgag
    25861 ccgtcaaaga ccatcagcag ccccgtgccc tcctggccgg ggcgcaaaag ctcgtagccg
    25921 cgcgagttcc agcggatgcg gtaggcgccg ctgtcggagg aggattcggg attgtactcc
    25981 ggcggcacga aaggcgcgcc gatgaccgcc cgctcctcga tcggattccg atactgcaaa
    26041 tcccgcagca ccttgagttc gtcggcggag gagtcatccc ggcggatgaa gctcatctca
    26101 ttgttggccg ccgaccagcg gtgcaggccc tggttgagca tctccacgcg ctccatcgcc
    26161 agcgtctcct gaccggcgct gtaagcgctg ttcatgctca ggatggcaac gcccgccagc
    26221 acactcatga tggagatggt gatgaccagc tcgctcagcg aataacccgc cgcgcgcccg
    26281 ttgaaacggg gcgcgcgggc ggctgcggct gggggagcga acggcatggg tggaggtttg
    26341 ccggggttgc tttcaatagg gaatgacgcc ggatggaccg gtgagcgtgc ttttctgcat
    26401 ggtggtgatg ccggaaggca gggtcaccgt gtaaccggat ggcatgtaga ggctcagcgt
    26461 gttttccgcg tagctcaggt aggggcggag aagattgaat ttcgcctcgt tcgaagccgc
    26521 gcctgtccag tccaggcccg cctgggtgcg cccgcgcacc tggatcagcg tggcctgcgc
    26581 caaattcaga gcctccgccc gcgatatggc cagattccgc tcgctgtccg agcgcatgcg
    26641 cgtgacggac gggatggcca ggaaggcgac aatgccgatg atggacacgc aggccatcat
    26701 ctccaccagg gaaaagccgc gtcgtgattt ggatgccggg tgtttcatgg ttgagcgttc
    26761 agtcattgac cagttccacc ctcggcaggt cgccatcctg ccaggcgggg agggtgaggt
    26821 gttgtttggc gccggacagc gcggcggttt tcagccggtc cgtgccagtc gtgtattcga
    26881 gaaaatgatc cgccgtggtc tggtccagaa acccggacct ggcgctgaca tccacagccg
    26941 aggggttcgg cagcaggaga ttgaagcgcg ccgaggtggt ggccagggca ttgtaagtgg
    27001 cgcggatgga gcccagcccc tgcacctggc cggtggtggc gttcctcgtc tgcgccatca
    27061 cccagacatg cagcgcctcc tggagcgccg cctgctgctg gcgcgccacc acccggttcg
    27121 catcccgcgc gccattggag atggcgctca cagccagggc ggacatgatg ccgatgatgg
    27181 cgatggtgaa aaccgcctcg acaaaagtaa agccgcgctt ggatggagtg ggtgtccgca
    27241 tatgaggtgg gtcgttggtg tgaaaaaaaa tccgccgggg attgctcccc ggcggatgtg
    27301 agtgcgcccg tggtgttcag attcgatgtg ccgtcacaag aaacctgggg ggccgaccgc
    27361 cgggcacaga tgagccagcg agggacgact aggaaccgtt ggctttgtag atgacctgct
    27421 tgttggtcgc gtcccagtcg aggtagctga ggacggtcgc ttcctgctcg tcatcaatgt
    27481 caccgacggt gaacaccttg tctttgaacg ggccgctcga aggagcgacg gcggtgccct
    27541 gggcggcggt cagcacggcg gccttgtcag cccagctggc gttgtagccg gcggcgatgg
    27601 cggcgttcat gacggaggcg acggactggg cgttgcgctt ggcggtggcc tgagcggcgc
    27661 tgtcattgat gttgccgatg ttcgggatgg cgatggcggc gatgatgccg atgatcgcga
    27721 tcacgacgag catttccacg agggagaagc cagccttgag gccggagcgg atgttgatgt
    27781 ttttcatggt atgtgtgttt gtttgggctg ttggtttgtc atcacccttt gaaaatccat
    27841 aattggacca ttgagtgaat atggaaatta taaacacggg caaaatggaa tcaaatagtt
    27901 ttttcataat tttcacactt ttctgacatt ttcatttcgt ggtatcatgc gagtgccagt
    27961 ctttgtcact tccatacctg cctcgcccct tgccagaatc gaaggagaag actcctttcc
    28021 catccatgga gcccaacctc aaaatcgccc tcgtcggcgc cggcatgttc ggcggagatg
    28081 tccatctgcg cgcttacgcc gaccttcaac gcttcggcat tgccggccaa ctcgcccgcg
    28141 tgggactgga caaattcagc cgcgacctcg caccggtgaa gttcgatctt gtcgccgtcg
    28201 ccactcgctc gcaaaaatcc gccgccaaat ccgccgccgc ttttaaagag tggaccggcc
    28261 atgaacccaa ggcctacttc ggcgacacgc cctgggatga cattctgcgc gacttccccg
    28321 acctcgatgt gctcgccgtc gccacgcccg accatctcca cacccagccc atcctcgccg
    28381 ccctcgccaa aggcgcgcat gtgctgacgg aaaaacccat gtgcctctcc atccaggagg
    28441 ccgacgagat catcgccgcc gccgaggcaa aaaactgcgt cgtcgccgtg gacatgcaca
    28501 agcgctacga tcccgaccac ctgcgcatcc gcgacgacat ccagaagcgg atcggcgcgc
    28561 cgctttacgg caccgcctat ctggaggagc cgctggaggt ttccaccagc accttcaagt
    28621 gggtggaaag cagcgacccc ttcagctacg tcggcccgca ctggaccgac ctcatctggt
    28681 cctattacaa atccaaaccc gcggccctca ccgccgtcgg ccagaagaag cgtctcgtgc
    28741 gcgacggcat caacgcctac gacgccgtgc aagtgcgcgt ggatttcgac aacggcatga
    28801 gcatcgcctt caacaacaac tggatcacgc cggccgactt cgaaggcccg gtgaaccagg
    28861 gacatgaaat cgtcggcgcc gacggcaagg tcgagagcga ccagcaatac cgcggcttcc
    28921 gcttctggaa cgccggcggc ggctgccgca ccagcaacaa ccacttcacc cgcgacgtgc
    28981 tccgcgccga cggcacccgc gcctacatcg gctacggcgt ggacagcctc accgtcgggc
    29041 tcgccgccat ctgccgggtg aagttcgccg gagaaagccg cgccgccgtg gcggacatgt
    29101 accccaccgc cgaggacgcg cgcatcacct gcgccatcgt ggacgccgcc gccaaagtgc
    29161 gggacctgaa cttcaaatat ctccaggaag gcaaaggcgc ccccgtcacc gcccgcttcg
    29221 gccaggacgg catcactctc atcgatccct gccgcgccgc cgaaggcccg gacatggtgt
    29281 ttcagcggat ttacaagggc gcgatttgac tctatcaaag gcccatgagc gaaccgcccg
    29341 acgaaaaaga agactccgaa gaagatgacc tcgcagaaga ggactttgaa gatgatgtca
    29401 cggatgaaga gttgctgcag cagatgcgtc cgtggcatcg ggcgctgatg gtgttcctgg
    29461 tggtaccgtt gatcaagacg tatgacttcc tcgaaggatt cttccgcaca aaatggagga
    29521 tcatcccgct tgatgagctt cccgcagcca ggcaacgctg gagcagtgta agcatgactc
    29581 ccgagcaggt gcgcgacttg atcaaacgac aggacggctt cctctgcgat gaatgcaaaa
    29641 tggagctcaa gactcaaagg caggatgccg aggaactctt cccgaatgtt tgggaggtgc
    29701 ggaaacacgg cagatgcgag gcatgcgggg cggatcgcga acattgcagc cggatccacc
    29761 acggctacct gctgttcaaa gaggatggcc gctggatggc cgtcttcgcc caagtgccgt
    29821 tcctccgccg atgctggctg cggctgcgag gctaagatgt cgcgcgcggt tggccgctcg
    29881 catggcggag ctccggccag gacggcacca ccctcatcgc tccctgccgc gccgccgaag
    29941 gcccggacgc cgtgttccgg aagatccaag acaaggctct gtgaggaccg cgctaacata
    30001 aacgccccgg tttcagtcgt gatggctgct ccagcagcac ggtgacagag ccggaccgga
    30061 ggacatcagg ctccagcgga tcgccgcccg gatcttcgtc aggggtttcg ggaggctcgc
    30121 ccgcgaagga ggcagcctcc tcgttcccgg ctttgccttt cggcggatgg gtgatcacag
    30181 cttcattcga ctcctcctcc acggggtcca cgccggcagc cggaaggccg gcggcagcgg
    30241 tggaaaccgg cggggcgcgg gttggtttga ccaggtttga ctgggtttga ctctccttgg
    30301 gtgaggcagg ctgatgatcc gtgccagcct ccgcatccgg gaagtccggc tgttccaagg
    30361 aaagaacatc ccacagcgcc tcccggatgg catcgcccgc ctcagcctgc cgcatcttga
    30421 tctggttttc gcagaatctg atgccggctt tctccgcctt gatctcgtcg cggagtttga
    30481 tgaggccggc ggcatcttcc tgcatctgcg cgccgagatt cgtgccggtg gagccggcgt
    30541 tttcctgcca ccccctctta tcctcgtctt cttcgccaaa aatgaggtga ttgacagcct
    30601 tcgtcagttc cccgggtgtt ttgctttcat ggaacaaccg ctgcatttgt tccctggccc
    30661 actcccgcca ttgctcatgg gtgcgcttcg tctgcgcctc ccgggccact tgaagctccg
    30721 cctttttgat ttcgagctgc tcgccctcca gtttgagacg cgccgcgccc tggtcaaagc
    30781 ggcgcagcgt ggccaccgcc tcgttcagct ccatcagccg ggcaagctgg cgcggcttgc
    30841 tccagtcctt cacctgcatc gtggtgatga cgctggcaaa gcgcccggcc agcagcgcgg
    30901 ccaggcgatc cgggagacgg ggagaggcct cctccaactc atccgcctcg tcggcgagca
    30961 gccgcaaccg ggccagccgt tcctgccggg cctcccactc caggaagccg ccctgccgcc
    31021 attcggagag attctgcttc aagatggggc gggctgcaaa gtcacggacg agcacttcga
    31081 gcacctccgg caggctgtgc agccaggcga gcaatggccc gcatggctcg ccatcggcaa
    31141 ggcgctggtt gagctgctcg cgcacggcct tgggcaggcg ggcgattttt ccagtgcgcg
    31201 ctttcatggg ttcagagggc atagttcttc ttgatgacct catggaaaaa agtgcccggc
    31261 gaggcggcgt tcaccagcct gaggtaaacg cgctccggca ccccgtagta ggtgtaggga
    31321 ccgttgttgg ggaaccagat gtggagaagt cggcgggcgg gatcgtagtc caccgccttg
    31381 atggcgtcag agttgagctt gatcatgggc gcaagcacac cacgacctgg ggacaaagtc
    31441 ttccgaacgg tcggctattt ggattcggaa gaatccgccc cagcccgatc caccatggca
    31501 agggccactc tccagcccct gggccggcca tccggcgcgc ttttgtcctg ccttgcgttg
    31561 ttcttcttga ggccgagcgc cttttgccgg tagtccgcca actcccggtc ctcctgcagc
    31621 ggatattcct cgggccgatc caccacctct ctgagcagaa ggcgcacatc cctgtccgtc
    31681 cagccatggc gcagaagcag gggccaccaa tgaatcacca gctcatccac ctgggttctt
    31741 ttgagcgcgc cccatttttc attcgactgc gccttgccga tcaggcccag ggccttgtct
    31801 ccctgatgct gcgcggcgag ttcttcatgc cggaactgcc accaatcttt gtcatgcggg
    31861 ttcagatccg caaaatttct ttgaatgaga ccgagctgca caagctcacg cctttcagga
    31921 gaggcttgca gggccagcgg cgccatgtgc gccacccaat gcgcctgcca gtgtgccagg
    31981 gcttcctgcc actccgcgaa ccgttgaaac cagtaacgca ggtgcttgag acacatcttg
    32041 aagtccatgc tcttttcctc ggcagctttc ctgcgggcgg agatggacac aggctcaccc
    32101 agctcatcct ccaggcgcat aaggtgcagc gccctcccca ccagctcgct ttccggggca
    32161 tcgcagttcg aataacggcc gatgcattca agaatccccg gcaactcatc ctccctggct
    32221 ttgccgaaga gctgctgcca ggattcccaa gcccggcccg tctgcaggcg ccggtctttg
    32281 cctttgcgct cagcatattc cgccaacgcg gcatcggagg catcccccag ccatgcctcc
    32341 aaaaggaagg cgatcacata aaacgcatcc ccactctgct tcgccatcgc cacagcctcc
    32401 ccatagtttc gaaatgccgg cagatgatcg ggtgggtcgg tgccattcat acccgccaaa
    32461 ctggccttcc agcgcccgaa aaaaagttca ttttttcgcc accccccgcc attgtcccag
    32521 cccctggtga aactgtgtgg gattctccat cccgtcatgc gcccacccag cctgccccag
    32581 agcaccccac acgacagtct tccgccctcc attcagagaa cagcgtcctt gcatgaaatg
    32641 actgcattag aaactcaatt caaagttcga aaagaatgtc atgttcaaaa gaattcatcc
    32701 ccctctttga gctcaaagag tcctttcaag acttccaaaa tcacgttcca gacctcaaaa
    32761 aggccattgt taggcctcaa acaacccttc taatttgcac gatagtgatg tatgcagata
    32821 aaatatagca taattggctt gaaaagttca aacctgtgat gtataatttt cctcgtcagt
    32881 ccaaaagaac ccggaccgct gaaaacagca atccgggctt tttggaaccg atgccacccg
    32941 agggcgacga tcagcgtgaa gaactggatt gtaactcttg gccggtgcgg tgcaacaact
    33001 tccctccagt ggctggcctg gagggttttt aacaccccaa aacaccaaga cagaacatga
    33061 ccgaagccga aaaaatccag ctagtctgga acaaaggagc catccccatc ccgcaagaag
    33121 ccgccaactg gcgcaaagac acctgcgggg cgtggatcgc acgcaaccaa tacggcaacc
    33181 gtgattccaa ttacggttgg gaaatcgacc acatcaaccc aaacggaggc gatgcgatta
    33241 gcaatctgca acccctccaa tgggagaaca acgtcgccaa aagtgacgga cggcacaagt
    33301 gcgtggtcaa aggtgtcgga aataagaact tcaggcaacc cgaagtgacg atctcccgat
    33361 gaccccgatc cccctgcacc acatcgccga tgtccgcatg ggggtcaccc tgcgggggcg
    33421 ggacgccaca cgtcccgccc ttcacgggtc ctgcttcatg atccgcatca gcgatctctc
    33481 ggatgatggg cacctcacca cggaggatct gcatcagttc gagcccggtg aggacatccg
    33541 gccggatcac ttcctgcgtc ccggagatgt gcttctgccg aatcgcggca cccgctgcac
    33601 tgcttatgtt tatgacctgc cgctgacaaa cgtgctcgtc ggagcgcagt tctacatcgt
    33661 gcgtcccgac accacgaagg tgagcgccga gttcctcgcg tggttcctcc gcacggaaaa
    33721 agccgccgcc cacttcgccc tccggcggaa gggcaccctc gtccagacgg tccagcgaaa
    33781 ggacgtgctg gagctggaac tccccttgcc acccctcgcc aaacagcatt ccatcatcgc
    33841 cctcgacgcc ctcgccatcc aggagcgcca gctttccatc cagcttgccg aaaagaaagc
    33901 cgcctacctc cagcgcgcca tgctccacgc cgccagtgcc tagccttcca tcagcggaca
    33961 agagaaccgc aggtagcctg caaggcaatc cgcgctcctc tcttttccat ctctccatct
    34021 ctccatctct ccatctctcc atttctccac cactccactt ccgcccccat gcccaaagtc
    34081 aaccaatccg aaatcaacga cgtcgcctgg cgtgcctgcg ataccttccg cggtgttgtc
    34141 gatgctgaga actaccggaa ctacatcctc gtgatgctct tctggaaata catgtccgat
    34201 gtctggcgcg accaccgcga cgcctggttg aaggaataca acggcgacga ggaacgcgtg
    34261 aagcgccgtc tggcccgtga gcgcttccag ctcccggacg gctgcgactt ctactccctc
    34321 tacgcccagc gcaatgaggc tgacatcggc gagcgcatga atgtggccct cgccggcatc
    34381 gaggaggcga acaaggccaa gctcgaaggc gtcttccgcg aggtggactt caattccgag
    34441 tccaagctcg gccagacccg cgaccgcaat gcccgcctga agctcctcct ccaggacttc
    34501 gccgatccac ggctcgacct ccggccctcc gttgttggcg aggaggatgt catcgggaac
    34561 gtgtatgagt atctgctgga gcgcttcgcc tccgatgcgg ggaaaaaggc gggcgagttc
    34621 tacacgcccg gccaggtctc cagcctgctc gcccggctgc tcgcgccgaa gaagggccag
    34681 accatctgcg atcccacctg cggctccggc tccttgctca tcaaagtggg ccgtcaggcc
    34741 gatgagcggg acttcgccct cttcggccag gagatgaatg gcaccaccca cgccctctgc
    34801 cgcatgaaca tgttcctcca cggcatggac aacttccgca tcgagtgggg agacaccctg
    34861 cgcaatcccc gcctcaccga gcacgaccag ctcatgaagt tcgatgtcgt cgtggcgaat
    34921 ccgcccttct ccctggacaa atgggggcac gaggacgcgg agaaggaccc cttccaccgc
    34981 taccatcgcg gcctgccgcc gaagtcgaag ggcgactggg gcttcatcac ccacatgatc
    35041 gagaccgccc gcgagcgcac gggcaaggtc ggcgtcgtcg tcccgcatgg cgtgctcttt
    35101 cgcggtgcgg cggagggaaa gatccgccag cgcatgatcg aggagaacct gctggaggcc
    35161 gtcatcggcc tgccggcgaa tctcttcttc ggcactggca tccccgccgc catcctgctc
    35221 ttcaacaaag ggaagaccca cggcgatgtc ctcttcattg acgcctcccg cgagtataag
    35281 gaggccaaga accagaacat cctcaccgag gagcacgtgc ggaagatcgt ggacacgtat
    35341 gcggcattcc agaccgtgga aaaatacgcc taccgcgcca ccccgcagga gatcgccgac
    35401 aacgacttca acctcaacat cccccgctac gtcgatacct tcgaggaaga ggccgagatc
    35461 gacctcgaca ccgtgaaggc cgacatcacc cgcctcgaat ccgaactcgc caccgtgcgc
    35521 gggaagatgc aggcttacct ggaggagctg ggggtatgaa ggcaccgagt tccatcctgt
    35581 tgcccatagc tctcgcgccc cttcagggcg cgacgcagag cgtgttcatt gacgtggtgt
    35641 tcccagggca cccagggcat cgctgcgctc agccctgggc tagagtcttt cggccctttg
    35701 ggccgccatg cattgccccc aaagggaggt cggaagtttt gggcgccccc tttgcaaagg
    35761 ccgtggggcc gctatgcatt gcccccaacg ggggcggaga ttccagccca gggctgaggg
    35821 agccccggcg accgatgccc tgggaaacga atgcccatgc cgttttcgtt tcatcgcgcc
    35881 ctgaaggggc gcgagagatt cacgccaacc cgacatcgtg gcctcgtccc actagcgccg
    35941 cacccgcaac ccgcctcgaa tccgaactcg ccaccgtgcg ggccgagatg ccccagggct
    36001 tctgtggccc tgggctggaa tctccgcccc cttcagcggc tcatctggcc caacgggcca
    36061 acagaaccca gcccagggct gagcgcagcg atgccctggg tgccctggat tcgacggttc
    36121 agcccgtttt catggcatcg cgccctgaaa gggcgcgaga ggttcacgcc aacccgacat
    36181 cgtggcttcg tcccaatagc gccgcacccg caacccgcct cgaatccgaa ctcgccaccg
    36241 cgcgggccaa gatgcaggct tacctagagg agttgggggt atgaagctgc caaagggatg
    36301 gaaaaagaag cggttagcag atctggtcga tccagagcgc aagattactt acggcatcgt
    36361 gcagccggga gagttcacgc ctgatggtgt gccgcttgtt cgcgggcaag attactcctt
    36421 tggttgggtg ccgcttgaaa agctttttcg agtttcggcg gccatcaatc atccttatcg
    36481 ccgttcgcgt ctaattggag gtgatttggt tatgacgatt gtgggagcag gcacaggcaa
    36541 cgttgcccta gttcctgact atgtcagcgg tgccaacatc actcagacaa ccgcacgcat
    36601 tgccgtggac caatcacttg ctgacacaaa ctacgtcagg caccaattac agtctcaaac
    36661 cggtaagcgg gaggtttatc gatatgtgaa aggcggggcg caaccagggc tgaatatcgg
    36721 agatgtagag gaattcttga tccctctccc ccccctcccc gaacaacgca agatcgccga
    36781 catcctgacg acgtgggacg aggccctgac ccagctcgac gccctcatcg aaacccagga
    36841 gcgccgcaag aaggccctca tgcagcagct cctcaccggt cgccgcaggc tcaagggctt
    36901 tgaccaatcg aatggcaaga ccgccagcga ccgatttggc acttatcccg ccgattggaa
    36961 gcgtgtgcgg ctcggtgaag tcacgaccga agtccccacc aagaacgctc aaggcaccaa
    37021 actccccgtg ctctcctgca ccaagcatcg cggcctcgtg ctgtccgaag agtatttcgg
    37081 caaacgtgtc tatgccgagg acaccagcgg ctaccgcgtc gttcagcgcg gcgagttcgc
    37141 ctatgccacc aatcacatcg aggaaggctc catcggttat cagaacctct gcgatgccgg
    37201 gctcgtcagc cccatctaca ccgtcttcaa gaccacggag gccgtggatg acagctacat
    37261 cttccgggtg ctgaagagca ccatgctcat tcacctctac cagatcaaca ccagcgcctc
    37321 cgtggatcgt cgcggcagcc tccgctacga cgaattctcc cgcatccaca tctggctccc
    37381 cggcaaagaa gaacaaaccg ccatcgccca agtcttcgac accgccgacc agcaactcac
    37441 cctcctccgc acccaacgca gcgccctcga ccagcaaaag cgcggcttga tgcagcgcct
    37501 gctgacgggg aaactccgag tcaagccatg accgaagacg cataaccatt tctcacctga
    37561 ttcaatgttc atcaaccctc catctcatcc agcttcggac gcctctcgcg ccccttcagg
    37621 gcgcggtgaa atgtgggcgt tgaatggcac gcaacccagg gcatcgctcc gctcagccct
    37681 gggctatgtt ctttcggccc gttgggccgg gttagcgtat cgccaacaag gagcgggaat
    37741 ccgtgtccat gccgtttcgg cctgttgggc cgtggttacc tgtccccaaa gaggaccaag
    37801 attccaacgc aacgcccccc agacccgtgg ggccgagatt cggtgtcccc aaaggggacc
    37861 gagattccag cccagggctg aggaagcctt ggcgaccgat gccctgggaa acgcacaccc
    37921 atatcgatta tttgatcgcg ccctgaaggg gcgcgagaga ggaccgaacc cacccctatg
    37981 aaaaccttcc ccgacatcga ccgcctgaag gccacgctgg accagcttcg accgctggac
    38041 ccggcgattg tgcgcaacct gcgggaagac ctcatcgtgc gctggaccta ccattccaac
    38101 gccatcgagg gcaacacgct cacccttcag gaaaccaagg tggcgctgga gggcatcacc
    38161 atcggcggga agtccatccg cgagcatctg gaggccgtga accacaagaa cgccatcctg
    38221 ctgctggaag acctcgtgca gaagaacgag ccgctgacgg agtggacgat caaatctctg
    38281 caccagctca tcctcaaagg cattgatgac gacaacgcag gccgctaccg caccgtcaac
    38341 gtgcgcatct ccggtgcgga acatctgccc cccgatcagg tcctgatccc cgagctgatg
    38401 gagaacttca tcacctggta tcaaaccgag gcgatggccc tgcatcccgt cgaacgcgcc
    38461 gcccgcgtgc acagcgactt cgtcaaaatc catcccttcg tggatggcaa tggccgcacc
    38521 gggcgcctgc tgatgaacct ggaactcatg aaggccggtt atccgcccgc cgtgctgcca
    38581 gtggacgtgc ggctggccta ttacacgttc ctcgatgccg accatgtgcg cggcgagaag
    38641 gattacttcg tgggcatggt gtcccgcatc gtgaagcagg gcttccgccc ctatttccat
    38701 gcgctgagct tgccgtggga ggatggggta tgagaggacg cggaaatcag cgaacacctc
    38761 tcgcgcccct tcagggcgcg gcggaatcac aatgtttgcg cgtcgtgaac ccagggcatc
    38821 gcttcgctca gccctgggct agattctctc ggcccgttgg gccggtgcat acgactagcg
    38881 ccatggtccc acgacgcagt gggccggtgc atacgactag cgccatggtc ccacgacgca
    38941 gtgggccggt gcatacgact agcgccatgg tcccgcgatg tggtggggcg gtccacacaa
    39001 ccttcgccat gatctctcgg cccgttgggc cggtgcaacg cacccccaac gggggccgag
    39061 attccagccc agggctgagg gagccttggc gactgatgcc ctggataacg cccgcccacc
    39121 ccgttgtcat tgcaccgcgc cctgaagggg cgcgagaggc tctcatccac tcctgacgtg
    39181 ccattccaac cgccatgccg caatcactcg ccaaactcta tgtgcacctg atcttctcca
    39241 cgaagaaccg agagcgcgtg ctgatggacg acattcggca tgacctgcat gcttacatgg
    39301 gcgggacatt gaagggcatg gattgcctac ccattgaaat caactcggag ccggatcatg
    39361 cgcatgtgct ctttctgctc gggagaacga tggctctgag cgaggtggtt ggcggtttga
    39421 aaaaaagcgc cacggaatgg ctgcgggagc gcggggaagc ctaccgggga ttccattggc
    39481 aggccggcta tggagccttt tccgtgagcc aatcggcagt cgaagatgtc cgtgaataca
    39541 tccgtaacca acgtgagcat cacggtgtga agacgtttca ggacgagttc cgcgcgtttc
    39601 tgcgcaaata cgaaatcgaa ttcgatgaac ggtatgtgtg ggattgatac acctcgccca
    39661 acgaagaatg cctctcgcgc cccttcaggg cgcgacggaa tcggtgtgtt atccacacga
    39721 cgtcccaggg cacccagggc atcgctacgc tcagccctgg gctatgttct ttcggcccgt
    39781 tgggccgcga ttgatcgccc ccaacggggg ctgagattcc agcccagggc tgcgggagcc
    39841 gtaggcgacc aatgccctgg gttcgaaggg catgagggag ccttagcgac cgatgccctg
    39901 ggagccgaac atccattacg cccccatttc atcgcgccct gaaggggcgc gagagcttac
    39961 cgcaccaccc aatgcctccc tccttccaag aagccctcat cagccagatt cccgcgctgc
    40021 ggatgctcca gcaccttggc ttcacctacc tgagcccgga ggagtgcgcc gtggagcgca
    40081 acggtcgcct gggccgggtg atcctcgaaa aggtgctggc cgggcagctc cggcgcctga
    40141 acaagatcag cttcaaaggc cgggaggtgg ccttctcgga ggaaaacatc gcccgaggca
    40201 tcgagtcgct gcgggacctg ccttttgaag gcctcgtgcg caccagcgag aaggtctatg
    40261 atttgctcac cctcggcaaa agcctggacc agaccatcgg cgacgagtcc aaaggattca
    40321 cgctgcgcta catcgactgg cagaacccgc gcaacaacgt cttccacgtc accgccgagt
    40381 ttgaagtgga gcgcagcggc agcaaagcca cacgcatccc ggacatcgtc tgttttgtga
    40441 atggcatccc cttggtggtg atcgagtgca agcgcccgga cgagaaagac tccctgacag
    40501 acgcgatgaa tcaaagcatc cgcaactggc aggcggacga gatcccgcag ctcttcctct
    40561 acagccagct cgtgctgggc ctgaacaaga acgatggcag ctatgccacc acgggcacgc
    40621 cgctgaagtt ctgggccaag tggcgcgaga tgccggacgc gacggacacc gtgcgccggg
    40681 tgatcaacac tccgccacgt ccagaggacc acgcgaagct ctttaagggg ctgtttgcct
    40741 atgcccgtga ggccttcgag gagcagatga tgcacgagcg ggaaccgacg gagcaggaca
    40801 agctgctctg gtccctctgc cgcccggagc ggctgatcga gctggcccgg cagttcatcc
    40861 tctacgacga gggcggcgcg gtgaagaagg tggcccgcta tcagcaatac tttgccatca
    40921 aggccacgct gggccgcgtg aggcagcggg atgtgcaggg ccgccgcaag ggcggtgtca
    40981 tctggcagac gcaaggcagc ggcaaatccc tggccatggt gctgctgggc aaggcgctgg
    41041 cgctggatga gagcatcagc aacccgcgca tcgtcatcgt cacggatcgc atcgacctcg
    41101 acgagcagat caccaagacc ttccaccaat gtggcaagga cccgcagcag gccaagaccg
    41161 gggctcatct catcgagctg ctgcgtgatg aacgcgtggc cgtgatcacc accgttctct
    41221 tcaagttcga agccgccgcc aaggtggccc gcgagctgca caagctgaag gacgatctct
    41281 ttgtgctcgt ggatgaaagc caccgcagcc aatacggcga cgccaacatc cagatgcaga
    41341 aggcgctgcc caatgcctgc tacatcggct tcaccggcac gccgctgatg aagaaggaaa
    41401 agagcaccgc gacgaagttc ggcggcttca tcgaacccgc ctacaccatt cgcgatgccg
    41461 tggacgacaa ggccgtggtg ccgctgctct acgaaggccg ccacatcgtg caggaggtga
    41521 accagaagcc ggtggacacg atgtttgagg ccatgtgcga gggcctgacg aaggaacaga
    41581 aggccgacct gaaacgcaag ttcaccgccc gcgacgagct gaaccgcctg cacagccgcc
    41641 tttacctgat cgcctgggat gtgagccagc atttctccaa gcagtggaag ggcacgggct
    41701 tcaaaggcca gctcacggcc cccggcaaga aggaggcgct gctgctgaag accttctttg
    41761 atcagttcgg caaggtgagc acggaggtca tcatctccgg acccggcgag atcgagaacg
    41821 ccgaggagca tcaaaccgcc gagaaggatg agagcgtgga actcttctgg aaggccatga
    41881 tggccaagta tggcaccgag aaggaataca accgccgcct gatcgacgcc ttcaagaagg
    41941 gcgatgaacc ggaaatcctc atcgtggtgg acaagctgct caccggcttt gacgcgccgc
    42001 gcaatgtggt gctctacatc gcccgcagcc tgaaaggtca cacgctgctg caagccatcg
    42061 cccgcgtgaa ccgcctgcac cccggcaagg actacggcta catcctggat tactacggtg
    42121 tcgtcaccga actgcacgaa gcgctggagc tttacagcag cctggaagag aagttcgacc
    42181 aggaagacct tgaaggcacg ctcacgaaca tcgccgaaga gatcaagaag ctgcccgcgc
    42241 tgcatgatgc gctgtgggac ctcttcaagg aggtaaagaa caagaaggac gaggagtcct
    42301 ttgaggtggc gctggcggac aaggacaagc gcgaggactt ctacacccgc ctgtggagct
    42361 tctgccgcgt gctgaagatg gcctcctcca gcatccactg ggtcaccgag acacctgccg
    42421 agcaggtgca gcgctacaag gcggacgcgg cctttttcca gaagctccgc gccagcgtga
    42481 agctgcgcta tgcggaggag atcgactacc gcgactacga gaagcagatc cagaagatgc
    42541 tgaacaccta cgtgcaggcc gacgaggtga tccaggtggt ggaaccggtg aacatctttg
    42601 agcgcgaggc cttcgaggcc gaggtggaca aagccaagtc tccccgagcc aaggcagata
    42661 ccatcgccaa ccgcaccaag aagacgatca cggagaagat ggaggaagac ccgttcttct
    42721 accgcaagct ctccgccctg ctggaacaag ccatcgccga ctaccgagcc gaacgcatca
    42781 gcgaagccga gtatctccaa cgcgtgaccg ccgtgatggc ggaagtgcgc ggtggcaaca
    42841 ccaaggacgt tcccgatgtg ctcaagcaaa acgatctctc ccgtgcgctc tttggtgcgc
    42901 tgaaggagca gatcgcgcca aaggcaggca cgtccggaga ggatacaggc agtgttttgc
    42961 gcgaagacgg ccccgcctac ggtggcgaag cgcaacgtcc cgcgccgcct gcggacgaga
    43021 ttctggccga agcggccgtg agcatggacc agatcatccg caagcatgcc gtggtgcgct
    43081 ggcgggaaaa catcgacgcg cagaaccgca tgcgaaatga cctcgatgat ttccttttcg
    43141 acctgcaaaa ggagaagggc ttcacgctga gctatgcgca gatggatgcc atcatcgaag
    43201 ccgtgatccg cattgccatg caccgcaccg acgatgtcta ggcccgagat catcgacacg
    43261 cctgccggtc cggcgcagct caagcgctcg caccgcaaga cgctggccat cagcgtgctg
    43321 cccgatggca cgctggagct ggtggctccc gatcacgccc cgctcgaagc gattttaccc
    43381 cgagtggcca aacgcctcgg ctggatcaag acccagcgcc gggccttccg cgagatgaac
    43441 gccacccgcc cggcgctccg ctttgtcact ggtgccaccc accgctacct gggccgccaa
    43501 taccggctca agatcgcgaa gggcgaggcc caagaggtgt cacttcgagg agccttcttc
    43561 cacgtttcga tccccaagac cggtgagaat gaggtcaaac aagccctcga agcctggtat
    43621 cgtcgccagt ctcgcctgca attcgagcgg cgacttgcct catggtccga gtggtgccgc
    43681 caccgcaggc tgccgcagcc caagctccgc ctccgccgca tgcccaaacg ctggggcagc
    43741 gccctccccg atggcaccat ctgcctcaac cccgagctga tccaggcacc gagcgcctgc
    43801 atcgactacg tcatcaccca cgaaatctgc cacctcaaac accccgacca cggcccgaag
    43861 ttccgcgcct tgctccagca gctttgccct gactggaagc ggctgaagga gcggctggaa
    43921 agcacaggct aaagaccgaa ccacgaagca agaacgagga actgagcctc tgacatctga
    43981 ctcgccagat tgtcaaccca gctgttcccc cgcccggggg gatggtccgg tgccgtggcg
    44041 accgcattga ccgcattgac cgtttctccc cgcccggggg ggatggtccg gaccagggtt
    44101 ctgggctgtc acttgcgaca cggtttcccc cgcctgccat cgtcgccgtc ctcacgggcg
    44161 gcggggccat gccggcgcat gaaccgctgg cgcggaagct gcgcggccag gcattcaacg
    44221 tgaaccgccg ccctgcgttt ctgaaggtct catccgcggc ttgaacaggc ggacaccggc
    44281 agcctggcgc gtgcgcgcct tttcactggc tttcccggcg cgtctgtggt tcatgcgcgg
    44341 catgaacgag ctggaggcga atccctggac gactctcgaa acgcgcaccg cctacgccaa
    44401 cccgtggatc cgtgtgcggc atgacgaggt gctcacgccc gcgggcaaac ccggaattta
    44461 cggcgtggtg gagtatcaaa accgcgccgt cggcgtgctg ccgctggatg aggagggcaa
    44521 cacctggctg gtgggccagt ggcgctactg ccatgaaagc tacgaatggg aaatcccgga
    44581 gggcggctgc cctcctggcg agaatgccat ggagtgcgcg cgcagggagc tgctggagga
    44641 gacgggcatc gtggcgggtg acatcgagcc gctgctgacg ggtctccagc tctccaactc
    44701 gacgacgaac gaggtgtgcg acatttttct ggccagggtg gcgggcttcg gggaggcgcg
    44761 gcccgaggag acggagcggc tgcaggtgcg caagctgccc tggcgcgagg cgctggcgat
    44821 ggcgctggac gggcgcatcc gcgacagtgt gagcgtgctg gcgctgctgg cgctggcgaa
    44881 ccggggaggg cgctaacatg catgcggtgc tcacgccctt tggcagcgcc ggggatgttt
    44941 ttcccctcat ccggctgggc cgtctgctga gggcacgcgg gcaccgggtg acgatggtga
    45001 cggcctgcat cttcgagaag gcggcgcggg atgccgggct ggaattcgtc ccgctgggca
    45061 aaccggagga cttcgagcgg atgattgccg atccgcgcgt gtggcggctg ggttccggca
    45121 ccaggctggt ctttgaattc gcgggacatg cggcgggcgc ttatctggcg gagatcgaac
    45181 gccttgcgca ggctggtgac aggcccagtc tcctgctcgc gccatgcacg gccttcggcg
    45241 cgcggctggc gcgcgaggcg ctgggcatcc cgctggtgac ggtgcatttg cagccggcgg
    45301 tcttcctcag cgcgcacgag cctccggtca tccttcccgg catggagcat ctgcggcggc
    45361 tgcccttgtg gatgcgcagg ctgctgctgc gcgcgccaaa cccggcggac ctctttgccg
    45421 gccccttggt gcgcgccttg tgcgcgaggc gcggagtgcc gccgccgcgc agcctctggt
    45481 gggactgggg gaactcgccg gacggtgtgc tggcgctgtt tcccgaatgg ttcgccgcgc
    45541 cacagccgga ctggccgccg ggtgtgtttc agtgggactt cccgctggag gatctctcgc
    45601 ttgagcatga gttgcagccg gaactgcggg agtttctcgc ggcaggggag aagccggtgg
    45661 tcttcactcc gggcagcgcc aacatccagg cgcgccgctt ttttgaggcg gctctggagg
    45721 ccgtgacgcg cgcgggctgc cgcgcggtgt ttgtgacgcg cactctggcg caggtgccgg
    45781 cgcggctgcc gccatccgtg cttgcagtgg agtacgcgcc cttctccgcc ctgctgcgtc
    45841 atgcggccgc ctttgtccat cacggcggca ttggcacgct gtcccagggc tttgcggcgg
    45901 ggctgccgca ggtggtgatg ccgatggcgc atgaccagcc ggacaatgcg caccggctcg
    45961 aacaactcgg cgcgggcctg agcctgccgc cgcgccggtt taccccggcc agggtggccg
    46021 cggcgctgga cgagtgcctg cggaatcaaa ggctgatcga gtccgcccgc caatgcgcgg
    46081 cgcgcctcca cccgcgcgcg gacagcaggc cgatgctgga gtggctggaa aaccgggcga
    46141 gcccgcagga aggacgacca tgaatgaaac cggcatcccg gctggcggga acgcacagtt
    46201 tgacgcccgc cctggcagcg tcaaaggatg atctcctttc atgccggact tttccaccct
    46261 caccatcctc ggccccggac tgctcggagg ctcgctcgcg ctcgccatcc aggagcgcat
    46321 gccgggggcg cggctgcgac tgtgggcgcg gagggactcg gcggaggcgg agattcgcgc
    46381 gcgcggcatc cacgcggaat ttttcacgga tgccaccgcc gcctgccacg gcgcggattt
    46441 agtcatcctg gccacccctg tggagacgat gcccgctttg gcggcgcgga tcgcgctggc
    46501 ggacctgccg gctcacgcgc tcgtcaccga tgtcggcagc gtcaaaggca gcgtcgtcgg
    46561 tgagttggcc cccatcctgg gcggacgatt catcggcagc cacccgatgg ccggctctga
    46621 gaagaccggc atcgagaccg cgcgcgcgga cctctttgaa ggcgccgcct gcctgctcac
    46681 gcctcttgaa gggcaggatg ccgcgccgct gcgggccttc tggcaaagtc tcggctgccg
    46741 tgtgctggcg atgacgcccg aagagcacga cctcaaagtg gcgcgcatct cgcatctgcc
    46801 gcactggatg gccgtcgtca ccaccctcgc cgccctgcgc gccgacccgg acgccgcgga
    46861 ttcatcagga tggttttcgc ccagccacca ccagcgccca atacgtca
//
LOCUS       DAOMYJ010000012        46821 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Prosthecobacter sp. isolate AMR_MDS_5629
            NODE_299_length_46821_cov_20.338259, whole genome shotgun sequence.
ACCESSION   DAOMYJ010000012 DAOMYJ010000000
VERSION     DAOMYJ010000012.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867850
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prosthecobacter sp. (wastewater metagenome)
  ORGANISM  Prosthecobacter sp.
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Verrucomicrobiaceae; Prosthecobacter.
REFERENCE   1  (bases 1 to 46821)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 46821)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.30
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:34:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,742
            CDSs (total)                      :: 4,684
            Genes (coding)                    :: 4,676
            CDSs (with protein)               :: 4,676
            Genes (RNA)                       :: 58
            tRNAs                             :: 55
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..46821
                     /organism="Prosthecobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_299_length_46821_cov_20.338259"
                     /isolate="AMR_MDS_5629"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1965333"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1630)
                     /gene="dnaG"
                     /locus_tag="PLP58_02230"
     CDS             complement(<1..1630)
                     /gene="dnaG"
                     /locus_tag="PLP58_02230"
                     /inference="COORDINATES: protein motif:HMM:TIGR01391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_process: GO:0006269 - DNA replication, synthesis of RNA
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HRJ07372.1"
                     /translation="MARIPEETLQQVLAATNIVDLIGRSVKLRRAGSNYVGLCPFHNE
                     KSPSFNVRPSTNSYHCFGCGASGTAFRWLMEHEGLTFMEAVRRLAEAAGIRIQEEVWD
                     ANAEAAAKQRALMLKAHKEIAAWYHNLLLRSPLAAPAREYLKGRGISSDIAKRWEIGY
                     APASLELLRQWAASFKFGRDLLVEAGLLGWSEEDNAVYPRFRHRLMFPIRNENGEVIG
                     FSGRLLDPEAKTAKYLNTSDTPIFNKSRVLFGFDKARRFISKAGQAIVCEGQIDTIMV
                     HEAGFPNVVAGQGTAFTEYHAQALKRQADEVVLCYDSDNSGQDAVTKAFRILAPHGLI
                     VKVAALPAGDDPDTFIRNRGPEAFAELLKNARDFLDHHLTAAGARRDLTEMRERVRFA
                     REVVDLLRVFDSPLARQTAIQRVARSLDLPEDILLGLVKDADAEEKKKAGYKKDKEAA
                     PAPADPLATQDKTSLLLCHLALVDAEVLVWLRGTGMEHILSELPGSEMLARVWQGAFD
                     PLDASAFSVFLAGLDRDEEAAFTRLLPRPRPPGRL"
     gene            complement(1648..3723)
                     /locus_tag="PLP58_02235"
     CDS             complement(1648..3723)
                     /locus_tag="PLP58_02235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009960698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HRJ07373.1"
                     /translation="MRIPTFLVFLPLLAGAADVADVADVVVYGGTSGGITAAIQTARQ
                     GKTAILIEPTQFLGGLTTGGLGATDIGNKRAIGGMSREFYQKVFQYYSDPAKWKQETR
                     EAYFARKPHGNTGSEDTMWTFEPKAASEIYDAMLDTVKDRVTVVRGERLDLKSGVVKE
                     GARITRIVMESGRAFTGSMFIDATYEGDLMAKAGVSYHVGREANSVHGETLNGVQVGH
                     SHHHQFKVNVDPYVKPGDPSSGLLPGIETDPGVEFSGDRKVQAYNFRMCTTDDPENKR
                     AWEKPSNYDERMFELALRNVEAGDERISWAPAWMPNRKTDTNNNFAVSTDFIGMNWDY
                     PEADYATREKIWKAHEDWQKGLMWTYAHHPRVPERIREAFQKMGLARDEFLDNDNWPR
                     QLYVREARRMVSDYVMTEKNCRRQEIVEDSVGMGAYNMDSHNIQRYVTKEGFVRNEGD
                     VQVRSRPYPISYRSIRPRASECANLLVPVCLSSSHIAFGSIRMEPVFMVLGQSAASAA
                     VQAIEEGVAVQDISYEKLKTRLLADGQVLDFESPPILEVSFFKKADLPGIIVDDTEAR
                     LTGFDSEGHTTPGFVERGYRHDSDKDKGAQSARFTPELPKAGKYEVSISYSALGNRAT
                     NVPVTIRHAEGEVRVLVNQKQKPAGKNNFQPLGSFHFEAGKAGWVEIGNEGTKGHVII
                     DAAQWLPAP"
     gene            4273..5490
                     /locus_tag="PLP58_02240"
     CDS             4273..5490
                     /locus_tag="PLP58_02240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009960467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMP-30/gluconolactonase/LRE family protein"
                     /protein_id="HRJ07374.1"
                     /translation="MNNRRAFLATLASSAAASAFARDWTGLVPERYPDPDVIALEPEF
                     SKLIQGNAPLRRLHTGMLWAEGPAWNGSGNYLVWSDIPNNAQLRYLPEDGHVSVMRNN
                     SGNSNGNTFDWQGRQISFEHANRRVVRYELDGKVTVLADSFDGKPLNAPNDGAVHPDG
                     GLWFTDPGYGSMMDYEGNKGELHLKEAVYRVDPATGKIEKVTDELAKPNGLCFSPDYK
                     KLYVVDTGAPKNILVYDVVDGVKLGKGRVFAVNAGVLTDRGRSIGVRERGKPDAPVMS
                     PGSDRPNISWSVADLQVLPSDQIPAVTRNAPGPTQDGVKFDKVMGHFRGGSDGVRCDV
                     DGNVWASAGWVGDGYDGVHIFTPEGKLMGHIKLPETTSNLCFGGPKRNRLFITASQSL
                     YSVHVNTRGAHFC"
     gene            5497..5928
                     /locus_tag="PLP58_02245"
     CDS             5497..5928
                     /locus_tag="PLP58_02245"
                     /inference="COORDINATES: protein motif:HMM:NF014688.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SufE family protein"
                     /protein_id="HRJ07375.1"
                     /translation="MTLAEKQTALIEDLTLIENAQERLAALASYTGGVHLPEPERADD
                     QLVPGCVSRVWLHTALGAEGRARLRCAADSPMVAGLVALLCHLYDDAPPAEIAAVEPE
                     LWRACGFEKMLSPTRLNGLASMRAQIRDWAARQIPLAPSAH"
     gene            5952..6026
                     /locus_tag="PLP58_02250"
     tRNA            5952..6026
                     /locus_tag="PLP58_02250"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5985..5987,aa:Arg,seq:ccg)
     gene            complement(6127..7098)
                     /locus_tag="PLP58_02255"
     CDS             complement(6127..7098)
                     /locus_tag="PLP58_02255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009958791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HRJ07376.1"
                     /translation="MNILVTGGAGFIGSHTVERLLDEGAGTVTVLDSFNDFYNPAIKR
                     RNLEAVAGRVTLREGDLTDADFVSRVFEEGRFDAVIHLAARAGVRPSIAEPGLYIDTN
                     IKGTFHLLESARRTGCRHFVFASSSSVYGVNKKVPFSEEDPILQTISPYAMTKMAGEQ
                     MCSNYSHLHGIRTVCLRFFTVYGPRQRPDLAISKFTRLIEDGLPIDKYGDGTTARDYT
                     YISDIVDGIIGALNHRAGPLCGIFNLGGSQTVTLNDLIAAIESAVGKKARVNLLPDQP
                     GDVPLTSADVGKAVRELGFKPSTTIAGGIPRYVEWFRQTRAAGLAVC"
     gene            complement(7105..7434)
                     /gene="trxA"
                     /locus_tag="PLP58_02260"
     CDS             complement(7105..7434)
                     /gene="trxA"
                     /locus_tag="PLP58_02260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009958787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HRJ07377.1"
                     /translation="MASESVLTLDEANFQSEISGSNVPVIVDFWAEWCGPCRMLGPIL
                     DQLAQEKAGAVKVGKVNVDHNPGLAAQFNVRSIPMMVFIKNGEVRDTVVGVQSKDALI
                     KRLEALA"
     gene            complement(7514..8320)
                     /locus_tag="PLP58_02265"
     CDS             complement(7514..8320)
                     /locus_tag="PLP58_02265"
                     /inference="COORDINATES: protein motif:HMM:NF012797.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HRJ07378.1"
                     /translation="MVISSKDNDRIKAARSVRDGREPGMIFIEGAKLAAEALRSDIKV
                     EAAFITVDARGRDAAAGALLRDPRIKGAPVFEVTEPVIASISDTQAPQGIILLARRPA
                     LRPVTPSATLLLGLDRVQDPGNMGTLLRTAEAAGVDAVIPLGGCADVYSPKVLRSAMG
                     AAFRMPLAAAEGGDLTLPPGMKVAAATGEGDMDYCEFDWRVPVLLLLGNEGQGLRPEL
                     LARCDARLRIPMARGVESLNVAAAGAVILFEAARQRRQAGQKTAKARKSD"
     gene            complement(8405..9238)
                     /locus_tag="PLP58_02270"
     CDS             complement(8405..9238)
                     /locus_tag="PLP58_02270"
                     /inference="COORDINATES: protein motif:HMM:TIGR00730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HRJ07379.1"
                     /translation="MSKPQRHHDEGRASGTGPLPTPPEVTPEQRDEQRRFCGRLVDQT
                     LLQGPNRRLKELSLLFAVMRDFLRGFRALHFVGPCITVFGSARFKEDHPYYQKAREVG
                     AAIARRGFTVMTGGGPGIMEAANRGAYEAGGRSVGCNIELPFEQHHNPYLHRWVTMKY
                     FFVRKVLLTKYSYGFVIMPGGFGTLDEMFEALTLIQTKKIRNFPVVVVGREFWGQLRG
                     LVDDMVKNGTISPGDLDLILWTDDVEEAAGHLVHIGATQFGLTCEEPPHSSVWLGEKG
                     I"
     gene            9481..12066
                     /gene="clpB"
                     /locus_tag="PLP58_02275"
     CDS             9481..12066
                     /gene="clpB"
                     /locus_tag="PLP58_02275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009963105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HRJ07380.1"
                     /translation="MSPEKFTVKLQEAYNASQSIATRHGHQELNPAHLLLALLEQEGG
                     IAQPLFQKAAVNPEAVTVLTSSISQLLAKQPKVSGATGGLYLSNEFRDLMTKAEDEQK
                     KLKDEYLSVEHIILALFKTKSELSNVLKQAGLTYDTVSKALTAVRGSQRVVDQDPEGK
                     YQTLEKYGTDLTARAKAGKIDPVIGRDEEIRRVMQVLSRRTKNNPVLIGEPGVGKTAI
                     AEGLARRIVAGDVPDSLKGKRLISMDLGGMMAGAKFRGEFEERLKAFLKEVTSSEGEI
                     ILFIDELHTIVGAGKAEGAMDAGNLLKPQLARGELRCVGATTLDEYRKHIEKDPALER
                     RFQPVKVGEPTVEDTIAILRGLKERYEVHHGVRIQDGAIIAAAALSNRYITDRFLPDK
                     AIDLVDEAASRIKIELDSMPTEIDVIERDIMQLEMERQALKKEKDAASKARLEKLERE
                     IADQKEKSSALKAQWMKEKESVDAGRKVKEEIDRLRTEQEQAQRRGDFARAGEIQYGL
                     IPNLEKKLQSAPTENREPRTANTLLREEVTEEDIAKVVANWTGIPVSRLQEGERSKLI
                     KMEERLSARVIGQKDAIVAVSNAVRRARAGIQDENRPIGSFLFLGPTGVGKTELCKAL
                     AEFLFDDDSAMTRIDMSEYMEKHSVSRLIGAPPGYVGYEEGGQLTEAVRRRPYSVVLF
                     DEVEKAHPDVFNTLLQVLDDGRITDGQGRTVDFKNTVLIMTSNIGSAAIQDVSNAEQR
                     DALVRDSLKQFFRPEFLNRIDEIVIFDRLDAAQLDQIVKIQLQRVIDRLAKQNIHLTV
                     TDDTTRHLADAGFDPVFGARPLKRAIQKHLLDPLSLAVLDGGFKEGDHITATMSGGKV
                     VFEKM"
     gene            12145..12375
                     /locus_tag="PLP58_02280"
     CDS             12145..12375
                     /locus_tag="PLP58_02280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ07381.1"
                     /translation="MTEAERELTKRWVDSWAKAAPELQKVRDADIRAADTAGSMKVFT
                     GSATWAVKNRPAAAWSGLVEQQRLFAKAALAS"
     gene            12372..12950
                     /locus_tag="PLP58_02285"
     CDS             12372..12950
                     /locus_tag="PLP58_02285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ07382.1"
                     /translation="MNELTKLALELQSFCEERKWSFCIIGGIAVQHWGEPRFTKDVDI
                     TLLTGFGSEERFVDDFLAAYEARVTDARAFALQNRVMLLRSSQGIGIDIALGALPFED
                     EAVKRAMKIEMEPGASLRLCTAEDLLVMKAFASRPLDWNDVRGILVRQGTRQLDWKYI
                     YLQLQPLCEVKEAPEILTQLENLRLEVAQSEP"
     gene            12957..13310
                     /locus_tag="PLP58_02290"
     CDS             12957..13310
                     /locus_tag="PLP58_02290"
                     /inference="COORDINATES: protein motif:HMM:NF021915.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ07383.1"
                     /translation="MQALQRVTDRLAKQNIHLLATDDTTRHLAAPAFGARPVKQTIQK
                     HLLDPLSLAFLDGSFKYGDHTPATLKIGKVEFEKRWLDPARGYASGSIFSNRLHPAPI
                     TCRTQEQFFAILSKY"
     gene            13435..14031
                     /locus_tag="PLP58_02295"
     CDS             13435..14031
                     /locus_tag="PLP58_02295"
                     /inference="COORDINATES: protein motif:HMM:NF019265.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HRJ07384.1"
                     /translation="MTATPDLEAQQQQIEEDIKLLERISNRDAAAFQTFYSKYSGLIF
                     AAVSNVLNDHHDTEDVMQEVLVQLWNKAHLYEPRKGKPLTWLTTLARNRAIDRIRSKQ
                     RRARLNDDFEMENKKLQFEFEESGSEALEIKERDRVLLSAVERLNDEQREAIRLAYFD
                     GLTQAEVAERLNEPLGTIKARIRRGVNRLESLVRPRLA"
     gene            complement(14044..14982)
                     /locus_tag="PLP58_02300"
     CDS             complement(14044..14982)
                     /locus_tag="PLP58_02300"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family A protein"
                     /protein_id="HRJ07385.1"
                     /translation="MTSPLFTVFTPTYNRAHTLHRCHESLKAQTLRDFEWLIVDDGST
                     DGTADLVAGWQAEGRVSIRYEQRSHLGAHHVHNFCLEAARGRFWVKLDSDDACKPEAL
                     GRMWHVWQSIPENEREGFAGVTGLCEDQDGRLVGGRFPLDPLDCTSAELQYIHRVEGE
                     KWGCLRLDVLRQFPYPDNVPGDFIPESFIWSQVARRYKTRHFNDMLRIYWMDAPSLVH
                     GMRNPCRNAAGHRLMFQKILEFESGYMCDAPLMLLRGAGQYVRFSFLSGKGPLSQVRD
                     LPAVRARALWLAGLPIGLALLARDWLSGSLGMSQRA"
     gene            15149..16657
                     /locus_tag="PLP58_02305"
     CDS             15149..16657
                     /locus_tag="PLP58_02305"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HRJ07386.1"
                     /translation="MNPAPPWISVIAWLSLVPAAFAVPAQQKPDMLVILADQWSPRYT
                     GWDNPQVRTPHMDRIAAEGMIFDACHTTSPVCMPARVSLITGLYPHNGGHALWGNGAQ
                     FYPKPEDAPMFLDIQRAGLTTAQIGKTHWTAGPAWHEHFKNSGAFFKALGLDHVADIP
                     GPPDSVNGRDPYSRHLRERGLLQAVADDLHGRYIRGEYEPRASVVKPEDYHDVFTTDL
                     AAAFIRAQPQDKPLCLVVSLHSPHPPLDAPGDYAAMFDPEKLTLPPSVPERYLREGRA
                     MDHAKTRRMLANYLGKLALVDDCVAKLVEALKARGTWDGAFVAISADHGEMMGAHGYL
                     TKGRFYDESVRVPLVLRWPAQVKPGRSKAPVQMMDIYPTLVEAAGGELTPGRFAKSLL
                     PIARGEKDRVRPIAISEIGDKAPLRIMARDDRFKYWADEEREYLFDLESDPLEMHDLS
                     TAPDHRETLHAMREKLLTHLRSTQSNLSAGYKPKVQRLREAEARKAASGAKP"
     gene            complement(16815..21056)
                     /locus_tag="PLP58_02310"
     CDS             complement(16815..21056)
                     /locus_tag="PLP58_02310"
                     /inference="COORDINATES: protein motif:HMM:NF024589.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HRJ07387.1"
                     /translation="MSKTIRIFISSPGDVSAEREKARQVITQLERRYDGAVKLVPVLW
                     EDLPLGADLSFQEGIDHILSGGGGIDLAVFILWSRLGSPLGARISRSDGSGYRSGTER
                     EWDMMMEARRQSGGRRPHILAYLRQDEAGFKRRLQEMPAERLPEEIQQQLLAQEFVRE
                     VFHDPETRANLRAYHTFAEPLTFASRLRVHLREFLDTMLGEELAGARLWEGSPYLAFD
                     VFEPAHAEIFHGRERELADLEDMLRSKPEQAFVVVVGPSGSGKSSLVRAGLAASLARF
                     NLDESVAQWRVAVLTPVRCEGDLLLGLARVLCSGSCLPEARENGTTPESLAAAFAESP
                     GSALRLALLPALDRAARACGGHIKLLLIVDQLEETHTDPRFGPATRDAFFRCLQVLAA
                     SGRFWIAATLRSDLYGALQRDPAFLELKGAGGQFDLPPPAPSAMRRMITAPARLAGLV
                     FEKDERTGATLDQLLLDEASRHPDALPLLEFSLHELYERRQNRTLTFAAYRDMGGIAG
                     AAGRRAGSVINSLPADVRAALPALFQRLVALDPLQERQPVRRASVYDPDDVSSPGGRL
                     AAALVLARLLTTGRDSERPTLTLAHEALLGAWGDLAAWVRENHHLLRLRSRVEQAHAR
                     WAEGGRNPSLLLPPGLPLEEGCRLIATAPVLLNSELSEFIELSSRSFQQARNKNRRRV
                     ALTLSSLALLGIVGAIAAVWFAERERARTDRLAAERLLGALLSKDVLQLANETGRFDM
                     AEKIAANLKNYMDTVPLHGGRRAEDVIVRLDLNESTALILQNSGDKERAMLQARRQVE
                     MAEELLKTKPDDEEALYRLCRAISGLAPMPHSGDQADERLALLERREALFQAAGPALG
                     EARWQSLLGGQQNQRAEIVSARASALLSQAANDFQAGRIQDYEKALAEIRPQIEELSR
                     MIFDHRSKAREHYRKARELEPNNNDYLSQLSYALLALADDLRSAGERLKTGPALSQRK
                     DLVAEATELARQYLQRLPEHDYANYLMINCMGAAAKFDFESGDAAKALSWLEDSLELM
                     RSMTLRSRDHPSRVQALVFQMQQLADLKAKSGDFTSAGKLLQQARDWLPKLQAHPKFK
                     DTADALEKLIEITQRNFAGREDQSLLLDQIRGNHASQLAELVLQSIPITEALDSQEKA
                     YRAAGLAGVLAELTDFWRPRLTRGSISDALRETLHVYQSQPRDGTLHSRYLWLGAGFL
                     AAALQHTTGQAEAALQTAHDCLSASVIADAKPGENAILAKLAKTLAAWESGAGKNDSA
                     VRVLQQWLSFALEARKNASPEEASSAGLSVVEALNEAGALHFQMGRLDEAENYYQQAL
                     EILLPATANASAGDKPAEWHARSAVHLNLAETALARIPSSPSGRTAALKASAAAHLDK
                     AEAILAELAKLAPPPPGLDPLTEFLRGLRAQLDKP"
     gene            complement(21156..22589)
                     /locus_tag="PLP58_02315"
     CDS             complement(21156..22589)
                     /locus_tag="PLP58_02315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007417954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1501 domain-containing protein"
                     /protein_id="HRJ07388.1"
                     /translation="MSRTFPRTEFPQETRRCFLGRSACVSLGGMALASLLAREAGGAG
                     VSRLAPKARRVIFLTQSGGPSQLELFDHKPGLRKWAGTELPESVRQGQRLTTMTANQK
                     QLILPGITRFFQHGRSGAAISEWLPHLGRVADDVCFIKSMTTDQINHAPAMTKFLTGH
                     QLAGRPSMGAWLSYGLGSVNQNLPDYLVLISKMLRPSDQPLYDHYWGSGFLPSRHQGV
                     KLRNSKDPVLYLRDPDGMPRELRRGMLDGLGELNQMRFESAGDPEILTRIRQYEMAFR
                     MQASVPELTDLSDEPDSTFELYGPDSRRPGSYGANCVLARRLAERGVRFIQLFHPDWD
                     HHSRLPSWCTARCRDTDQPSAALVKDLKQRGMLDDTLVIWGGEFGRGVAGQGQWDSPE
                     AGRDHHPRCFTLWMAGGGVKPGITYGATDDFSYNVVENPVHVRDLHATALHLLGVDHE
                     RLTYRAQGLDFRLTGVEEARVVRDALL"
     gene            complement(22683..24497)
                     /locus_tag="PLP58_02320"
     CDS             complement(22683..24497)
                     /locus_tag="PLP58_02320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ07389.1"
                     /translation="MKPALPKSALAALALALSTIHPVRAGVLEDWLKLLPPSTIAVIS
                     VKDSPELMADWEKSGIGRMMQDEAFKRWTAPMMKDGEAPWDTFARENFGESLHDNLAR
                     YPGASLAAFVADSPQQIEEQDGVPAFIAFSETGDKRKELEESMTREKELALAKEDGVK
                     ARTEEIAGEQVEILAKSEEPGALWESGHAFVGGVLVESNSAALMEQMILALKNGAAEN
                     PEAILGHIGRIEALHGGSPDALVYFNGEVLMQWAVTAAREFGATAAKGSPLPVTPDQV
                     IEALGLQELQSLALMMNLEDGQSVFDLAVLHASKPAGLLSLVRGGAGAVELPGFVPGD
                     VVSASAMRMSLLELWDKLLAIVQKMGPAAALLTGQLGIYEQQFGVKIRDDLFASLDDQ
                     YYEITDAADGVTSQVIAIKIKDGKRFGGAVEAVKKIVGAGFGAFDETEYLGHTIQSVK
                     TAGATAGSPGFAFCVTDTHLLMSTGPVALLQDVLARMRDPSGPSLWEDARAKDLMSRL
                     SPGYNGVGVTDGARIMKLLVDTIVMARSGLGGSTKAKGPKGGAAKSAEDAVDGIFDPD
                     AAPSEETWSRYFGTSVTGTYQPDDAIHVRFLTTPVEAR"
     gene            complement(24494..25465)
                     /locus_tag="PLP58_02325"
     CDS             complement(24494..25465)
                     /locus_tag="PLP58_02325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007418356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HRJ07390.1"
                     /translation="MIKNWTPLLAGFCAAVCQTVVAETSILRLKNGAVLQGEVLRERD
                     DSLLFDLGFQVLTLPRAEVVSVEKTGEEKAGPKADSILAEDVYFVEPAREPLPVDKNV
                     HRVGGAVVQVQTASGLGSGFIINKSGHVITNQHVISGEREITIVVYEQKDGGLEKRVF
                     PKVRIIAMNGFLDLAILQIDDPAAETLPFVPIGDSDSLTQGQQVFAIGSPLGLERSVS
                     QGIVSVRARDTRGAWSIQSTTQINPGNSGGPLFNARGEVVGVNNMKLAGVGVEGLGFS
                     IPANLLKLFLKNRDAFAFDPRNPNSGFTYLRPPAPGDKPAVITPQNP"
     gene            complement(25628..28348)
                     /locus_tag="PLP58_02330"
     CDS             complement(25628..28348)
                     /locus_tag="PLP58_02330"
                     /inference="COORDINATES: protein motif:HMM:NF019259.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1592 domain-containing protein"
                     /protein_id="HRJ07391.1"
                     /translation="MFPRHLHFLLIAALPCAARAAERGQEEFEKKIRPLLEQYCFDCH
                     ADGVDKGDFTFDEHKDYAALRSDFKLWDHVRQQLVTHVMPPEKKPAPVIEERDAMVAW
                     IDDAVFWFDPARPDPGHVTLRRLNRNEYNNTVRDLLFVDTRPAREFPPDDTGYGYDNI
                     GDVLSLSPMLMEKYLRAARGVAADAMEVRVSDHLDLEVSGRRFYRSKGDTREQGDLRW
                     FSGQSEIAHHVYPPAEGDYTLTLQLAAVRAGGEPAKLALKANGKEITTLDVTTGWKDE
                     DGPWQTVRQTVHLKAGDTKLALSLLNPRAEPAREGEKPGERRVVLAGMTLKGPHGLLA
                     PKGSRFVRWLLDNKPAGRPAMQLTGEDFDKGEGASSRDTGGILLASSGYVRHPLHLAK
                     AGKYKLTVKAGAQQAGDEPAKFDMRVAGKKIGAFSVTAKNQAPQFFTFEADLPEGAHE
                     LQIWFLNDFYNAETREDRNLWVHQFRIEGPLSEPAGVRAEDVPALVEKMTARLFRRPP
                     QPQEVKPWQDFAVLAMREGEPPLGALRFVLEGVLASPAFIFRSAPQPAGAVADGAAFI
                     DEFSLANRLAYFLWSAPPDDKLLELAGKGELRANLRAQIQRMTGEWKGAAFTENFAGQ
                     WLQLRDMDIVAPDTRAFPGWRGDIASSMKRESQAFFEHILRENRPVTEFLDADYTFLN
                     EKLSKWYGIGGVKGDKFEKVSLKGTPRGGILTHGSILTLTSGQTRTSPVKRGKYLLEN
                     ILGTPPPPAPGGVPPLDEGKVRRSNLTLREQFAQHREHTSCAGCHAFLDPMGFAFEHY
                     DAVGRWRDKDKNHPVDASGKLVRGQEFKDLAELRGILARDMPDEFARNLAENLLTYAL
                     GRGLAYSDKPAVQEITGKAKAAGYRFQDLILAVCESVPFQKMRAGERTGD"
     gene            complement(28626..29090)
                     /locus_tag="PLP58_02335"
     CDS             complement(28626..29090)
                     /locus_tag="PLP58_02335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009960484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly scaffold protein"
                     /protein_id="HRJ07392.1"
                     /translation="MYETALQSALENPQNLGEMPDADAVGTVGSPDCGDMVRMWLKFT
                     EKDGRKVIDKATFQSFGCQTAIAVASMATELIRGKTKEEALKMSAEELSAPLGALPPM
                     KIHCGQMVEGALKAALDAEQAVAAPALAGPTLSDSLAAAGRQAGRIKIVFDN"
     gene            complement(29304..30329)
                     /locus_tag="PLP58_02340"
     CDS             complement(29304..30329)
                     /locus_tag="PLP58_02340"
                     /EC_number="3.1.3.1"
                     /inference="COORDINATES: protein motif:HMM:NF020976.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004035 - alkaline phosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase D family protein"
                     /protein_id="HRJ07393.1"
                     /translation="MRRLIFAFLLPAIITTAADDKPLRHIVFGSCLDKVEHPMLDRAL
                     ALPMDLFIYMGDNIYADTRDMAVMRAKYSALAGSRFHQGLLARALVLATWDDHDYGVN
                     DGGADYPMRKEAQKEFHDWLGTPAGSPLRAREGVYHSRVFGPAEKSTQVILLDTRYHR
                     TPLKRVPKEQAGIGGSAVPNDDPDATLLGAEQWRWLEAELRKPARLRLVISSIQFAAA
                     ASGSESWANFPLEQRRMADLIRVTRAQGVLFFSGDRHWCELSTLTGNVPYPLHDLTAS
                     SMTQAHPRGTPTPNANRSLPATFHQPNAGALEVDWDAPDPLLTLRVVDQEGATRLEKR
                     LRLGELR"
     gene            30348..30506
                     /locus_tag="PLP58_02345"
     CDS             30348..30506
                     /locus_tag="PLP58_02345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ07394.1"
                     /translation="MKQQAKAKDGSSASAIFLAAAVLVPNWILNRAKPPSQMTRSGQA
                     RRVWLFQG"
     gene            30538..30837
                     /locus_tag="PLP58_02350"
     CDS             30538..30837
                     /locus_tag="PLP58_02350"
                     /EC_number="4.2.1.96"
                     /inference="COORDINATES: protein motif:HMM:NF002017.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008124 -
                     4-alpha-hydroxytetrahydrobiopterin dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006729 - tetrahydrobiopterin biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4a-hydroxytetrahydrobiopterin dehydratase"
                     /protein_id="HRJ07395.1"
                     /translation="MPVKITDDQLPRLLHDHPGWTVEGGQLARVFVFGSYLDGVEFAR
                     RVAIEAEALNHHPDIHIGWRKVTLRVSTHSARAITTLDFDFVERAGAVWAAFAGS"
     gene            30957..32228
                     /locus_tag="PLP58_02355"
     CDS             30957..32228
                     /locus_tag="PLP58_02355"
                     /inference="COORDINATES: protein motif:HMM:NF024753.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HRJ07396.1"
                     /translation="MKPGILIPALLACLSFQLAAEDWPRFRGPTADGRTSATELPLKW
                     SERENLLWKTELPGPGSSSPIVSGGKVFLTSYSGYGLDSREAGDAAKLKRHALCLDAA
                     TGKILWQHEIESAGQDKPYTGQYITMHGYASGSAVTDGSGVFFFFAHAGVHAFTVDGR
                     KVWEKSVGEKAHEWGAGSSPVLHGDLLFVNAALESDTLYALDRRTGKEVWSARGFPAS
                     WNTPVIVKLKDGSEELVVNASGRLRAFDPEKGGELWSCQAIRAAELCPSVVAHDGVLY
                     IIGSPRGNAMAVRAGGKGDVTQTHVLWQAQKGSNVGSPVFHEGHLYFVNDARGTFHCL
                     KADTGEDVYEERLPGSRDRWYASPILNNGRLYYTSRTSGTVVIPAAPKFEVLATNVIA
                     GDSSPFNASPAAVDGRIYLRSDRFAYCIGPR"
     gene            complement(32255..32331)
                     /locus_tag="PLP58_02360"
     tRNA            complement(32255..32331)
                     /locus_tag="PLP58_02360"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(32295..32297),aa:Asp,seq:gtc)
     gene            32498..33313
                     /gene="trpA"
                     /locus_tag="PLP58_02365"
     CDS             32498..33313
                     /gene="trpA"
                     /locus_tag="PLP58_02365"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009960762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit alpha"
                     /protein_id="HRJ07397.1"
                     /translation="MLENTNRLDSCFARLRAARKRAFVAYICAGDPTLKKTVDIVLAL
                     ERAGVDVVELGVPFSDPLADGVVNQMAADRALRAGATTAGVLEMIREVRGHSQIPVVL
                     FTYLNPVYAYGYERFHHDATAAGADGILVLDLPPDEAAENAELKPVPDLRHIQLIAPT
                     SSPERIASIAASAEGFIYYVSRLGVTGAQTEIAGGIAEQVAEIKKVSQVPVCVGFGVS
                     NPGQAAAIASMADGVVVGSAIVKLIEKNGASPDCTASVEAFVKPLVDAVKALP"
     gene            33343..34155
                     /gene="dapF"
                     /locus_tag="PLP58_02370"
     CDS             33343..34155
                     /gene="dapF"
                     /locus_tag="PLP58_02370"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012419106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008837 - diaminopimelate epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HRJ07398.1"
                     /translation="MTLSFTKMNGAGNDFVMLDNRSLATSLSREQIARLCDRHRGIGA
                     DGLLCVEPAEGKGDFKMRYYNADGGEAEMCGNGARCFGRFVNRLLHDELSFVQFETQA
                     GLISAEFEEEQVRINMSAPHSLKMAQELLVAGTTLTVHSINTGVPHAVVFVSDLESVP
                     VREWGAGLRYHEAFALKGTNANFVQVLGLDSIAIRTYERGVEDETLACGTGMVACALI
                     HHELTGVPGPVAVKVKGGDTLRVGFEEPAPHEYSNVTLLGPADFVFTGSVEV"
     gene            34225..34584
                     /locus_tag="PLP58_02375"
     CDS             34225..34584
                     /locus_tag="PLP58_02375"
                     /inference="COORDINATES: protein motif:HMM:TIGR02436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four helix bundle protein"
                     /protein_id="HRJ07399.1"
                     /translation="MEERFLDFVGRICAVVEALPDTKTGRHISGQLIRCGTSPAPNYA
                     EACAAESRADFTHKLGIVLKELRETRIWLKLVVKTGSMKKSKLEALIGECSELMNIIG
                     ASIVTTKANTPPKNRRR"
     gene            34671..35549
                     /gene="dapA"
                     /locus_tag="PLP58_02380"
     CDS             34671..35549
                     /gene="dapA"
                     /locus_tag="PLP58_02380"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009960759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008840 - 4-hydroxy-tetrahydrodipicolinate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HRJ07400.1"
                     /translation="MFAGTHTAIITPFRNGRIDEDALKRLVDFQFDNGVNGVVPCGTT
                     GESPTLDYDEHERVVKLTVEFTRGRGIVMAGTGANSTREAVEMTQEAEAAGATASLQV
                     APYYNKPTSEGLYQHFKTIADNTSLPIMLYSIPGRCGIEIGLDVLERLAGACPNIRAI
                     KEAGGNPERVSQMRQILPESFEILSGDDGLTLPFMSVGGVGIVSVASNVIPRQISDMV
                     NLALKGDYTGARAIHERYYPLFAAFLKLSTNPVPVKAAMSLAGHCANELRLPMTPLEE
                     TKLAELKATMEKLGLL"
     gene            complement(35627..36097)
                     /locus_tag="PLP58_02385"
     CDS             complement(35627..36097)
                     /locus_tag="PLP58_02385"
                     /inference="COORDINATES: protein motif:HMM:NF021193.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PGPGW domain-containing protein"
                     /protein_id="HRJ07401.1"
                     /translation="MDLHWLTDSAAWATVRSLSGWLTPQVLIIVSIASLAMFLASVFV
                     TPLLLIRMPADYFLRDKPHLWTRLKTASMPRRVVLLVKNVIGVFFVALGILLIFLPGQ
                     GLLTILVGIFLLDFHGKKELERKIISRPRVRQAINWLRRRNHRPDLILEEADAA"
     gene            36239..37132
                     /gene="ilvE"
                     /locus_tag="PLP58_02390"
     CDS             36239..37132
                     /gene="ilvE"
                     /locus_tag="PLP58_02390"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009959290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004084 - branched-chain-amino-acid
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain-amino-acid transaminase"
                     /protein_id="HRJ07402.1"
                     /translation="MAGTPLLIYLDGRIVPESEAKISVFDHGLLYGDGCFEGIRIYNG
                     RVFRLTEHLVRLYESARSICLTIPISLEEMEKATVETVAANGLRDGYIRLVITRGVGS
                     LGLNPYQCPKASIIIIASGITLYPKEKYETGLHLITCATRRPATAALSPQVKSLNYLN
                     NVMAKIECIQAGCEEGIMLNEQGYVAECTGDNVFIIKNGRVFTPTIASGALNGITRMA
                     VIEVMREMGLDVSEVTMTRHEIYTADECFLTGTAAEVIPAVQYDRRLIGCGKPGKLTL
                     EIIQRFKKLANSTGTPVPYAA"
     gene            37150..37947
                     /locus_tag="PLP58_02395"
     CDS             37150..37947
                     /locus_tag="PLP58_02395"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004799 - thymidylate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006231 - dTMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HRJ07403.1"
                     /translation="MHEYHRLLRKVLDHGSFREDRTGTGAWSVFGEQSRYDLGRGFPL
                     VTTKKLHLRSIIHELLWFLKGDTNVRYLQENKVTIWDEWADENGDLGPVYGAQWRRWQ
                     GSPGAEPVDQIARLVEGLKNDPHSRRHIVSAWNVPFIGQMALPPCHCLFQFFVADGRL
                     SCQLYQRSADLFLGVPFNIASYSLLTLMAAQVCRLQPGDFVHTFGDLHLYANHTEQAR
                     LQLTREPRPLPVMRLNPEVKDLFAFRFEDFTLEGYDPHPPIKAEIAV"
     gene            complement(38260..40260)
                     /gene="ccsA"
                     /locus_tag="PLP58_02400"
     CDS             complement(38260..40260)
                     /gene="ccsA"
                     /locus_tag="PLP58_02400"
                     /inference="COORDINATES: protein motif:HMM:NF013725.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcsA"
                     /protein_id="HRJ07404.1"
                     /translation="MKRLVLLSMLAFAALAPAADLPPLDLFRDKEVVETFASIPVQES
                     GRIKPLENVANYRLLRFRARRSIWLTENGEMDGKPVTDPAAQKPIVKEGGKPVKLNAT
                     EWLLMSWFRPDIARLVPLFKVDNSSAITELGLTARAKRDQYTFAEIEPARQTLMEKMG
                     EYREIPSKKQTPEQRMIVQLAANFLDYEMITGHFDFIRSPVGDKVDQLPAGIEKPVRL
                     SKSLNALASAVTASGGPPMQIPWFRDFGKAALGAMMSGNSEQQLRIFPPGPKVDEVWH
                     GPGEIIFGVFNDEKEAAAEQLAWLAVYEDVFLALPDAEKFKAAAKALLAKIQAAAKAR
                     DEGRFIALERHSIKADYFFYAQWIFLLGFVAVALAWIAPGSKLEKITKAASWLLLATA
                     TGCAVTGVVIRCVIMQRPPITTLYETILFIGASVALFGLAAEWITKRGLGLLVAAVGG
                     TACMFLAIQFEASEATDTLQQLQAVLITNFWLSTHVPLINLGYAACMVAALISMIYFI
                     QRLFGKIGPKSEEGRFLTRVAYGFIAAGLFLSLVGTVLGGIWANYSWGRFWGWDPKEN
                     GALMIVLMCLVILHARLGGYIREVGLHNCNLILGCIVIFSWFGVNQLGVGLHAYGFTD
                     GTWPKIYGFWGSQLALLVYGLYLVRADRSAASEAVSAVKAEA"
     gene            complement(40285..41595)
                     /locus_tag="PLP58_02405"
     CDS             complement(40285..41595)
                     /locus_tag="PLP58_02405"
                     /inference="COORDINATES: protein motif:HMM:NF016995.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein ResB"
                     /protein_id="HRJ07405.1"
                     /translation="MSSPKPNPLARLLAFLSSFGLATIVLALLLLITFLGTLEQAEYG
                     LVASQARYFESFLVDRIDIGACWRALALNAYWDIGNATFPLPILPGGYTLMAVLFVNM
                     FLGGIIRIRKSPKTIGVIISHFAILFMIAAGAVSYHFAVEGNMSLREGETSDEFLSFH
                     DRVIEIEKVQADEKAKRTALVIDESKYRDLSAGKGRTFTHESLPFDLMITNWKKHAQP
                     RRDRDGSRGDAIDGYFIQEVSMLDDSGGALADEQLASACAAIVKDKKTGAEQKGILWE
                     FAAAPWTVTFGDDKYLISLVHRTYKLPFAIKLDKTEQEKHPGTERARRFTSWVTKLED
                     GREEKRVITMNEPVRSGGYAVFQQTFDDGTRSRSGVKSSGFQIVENPSDHWPLISCII
                     VAEGLLLHFLMMLARFIKVGPWKFSISLILAFNAAFALAMWKLL"
     gene            complement(41704..42309)
                     /locus_tag="PLP58_02410"
     CDS             complement(41704..42309)
                     /locus_tag="PLP58_02410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ07406.1"
                     /translation="MPSCDRLGKSEAITESREISIHAPRPNVDIPSATRFFDDAREEQ
                     PQRPDFRSLLTWTVPEGWSENPEPDPMGMRLLDLRFGPNKEGECYISLMPGSAGGLDA
                     NVNRWRTQMDQPAYTAEELAALPKKPFFNREATFVAFDGDFKGFGADTAKTGYRMLGL
                     IHSTEQATIFVKLTGPKELVEQNTAAFDAFCQSIKPNVPRQ"
     gene            42744..43055
                     /locus_tag="PLP58_02415"
     CDS             42744..43055
                     /locus_tag="PLP58_02415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009960695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dabb family protein"
                     /protein_id="HRJ07407.1"
                     /translation="MQHNVYFWLKSGMTGDQVRVFETELAALTRISYLESGFCGKPAP
                     TEARPVTDHSFSYSLSLRFKSMADHDFYQKGCPDHQRFVDTCKPFFEKVVVYDSQPLG
                     N"
     gene            complement(43080..43616)
                     /locus_tag="PLP58_02420"
     CDS             complement(43080..43616)
                     /locus_tag="PLP58_02420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ07408.1"
                     /translation="MTAFWLMRPVLWPEKDPPPLAGAAPASSMPHPPAEPLPGERILR
                     SYASADSRAEDDLAAMAHAVANLALLVKGGDPFRLGANEEIAAALRGENRARIRFLPD
                     DHHAFDAQGRVVDRWGTPLFFHALSHERLDIRSAGPDRRMWTEDDIHRRHDGAYLGAE
                     ALNAPSLFEAEGAKPDRR"
     gene            complement(43676..45118)
                     /locus_tag="PLP58_02425"
     CDS             complement(43676..45118)
                     /locus_tag="PLP58_02425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009965541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1501 domain-containing protein"
                     /protein_id="HRJ07409.1"
                     /translation="MPAAMNRRRFLGQASCSALSGVSVLNTLLNLRLANHAAASGAPA
                     DDRTLVCLFLNGGNDSFNWLVPRDAARHAVYATTRSDLALGTGDLEVLDQDAGGDGQL
                     YGLHPSCQGLADFFNGTGVSGGQRRAAFLANVGTLIQPVTKAQYLAASVPLPRALYSH
                     SDQIDQWQTSVPQGMTQLSGWGGRAADVLHAVNTSALSMGISLSGNNLFQVGNQSAQF
                     VVTSSGALTFTGSGETSPAHPLFQKNAAHRSIIEQSYASLMQEAYAKLTKGSIELQEF
                     FLQQFNSYDDSPVAGLFPGGNWVAQQFRAAAKMIALRPALGLHRQTIFIGFGGWDHHG
                     ELLETQAGMLAVMDAALTGFQRALDQLGLADSVITFTASDFGRTLRSNGRGTDHAWGG
                     NALVMGGPVRGGRVFGSFPDLALDSSLDTGFGGRVIPSTSCDSFFAEMLRWFGVAAAD
                     MDYVLPNIANFYDPHSSSLPVGFLKPGTWS"
     gene            45271..>46821
                     /locus_tag="PLP58_02430"
     CDS             45271..>46821
                     /locus_tag="PLP58_02430"
                     /inference="COORDINATES: protein motif:HMM:NF025761.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="circularly permuted type 2 ATP-grasp protein"
                     /protein_id="HRJ07410.1"
                     /translation="MPSANPAARTATPPHEAPAGGGLLEGYRPQPGVHDEMLLPGGAL
                     RPHWRTFGSFLAQCGPEDIQARAAAIQRLLRDHGVTYNVYDDALGTSRPWSLDVLPFL
                     LGEDEWLHVSAGLDQRSRLLNAILADLYGPQTLIQAGLLPPRILHANPGFLRPAAGIT
                     PPGGRWLSHLACDLVRGADGRWLVLTDRAERPAGHGYTLENRVILSNVFPEEFNASRV
                     RRLAAHFDSQREMLQSLAPKTRHGRAGILMLTPGPRHETYYEHAFLARHLGFPLVEGA
                     DLTTRDRALYLKTLEGLHRVDVMVRHVDDRWCDPLELRAESLIGVAGLVEAWRNGSVA
                     LANGLGAGIVETPALHPFLPGICRHLLGQELLLPCVPTWWCGQQRELELLLAEPSRWV
                     VKPAFARGARDPVFLADLDSEQRERQLESIRAAPHEWVAQEVLPLSTTPAWTGSRIEA
                     RPLVWRAFAIARGEGFETMPGGLARVSPEPGRWLITLRSGGISKDTWVISSGAHPHAP
                     QAPAREAAA"
BASE COUNT         8457 a        15251 c        14799 g         8314 t
ORIGIN      
        1 ccagccggcc cggcgggcgc gggcggggca gcagccgggt gaaggcggcc tcctcgtcgc
       61 ggtcgagccc ggccaggaag acactgaagg cgctggcatc cagcgggtcg aaggccccct
      121 gccacacccg cgcgagcatt tcggagccgg gcagctcgga gaggatgtgc tccatgccgg
      181 tgccgcgcag ccagacgagc acctcggcat ccaccaaagc caggtggcag agcagcaggg
      241 aggtcttgtc ctgcgtggcg agcgggtcgg cgggcgcggg agcagcctcc ttgtcttttt
      301 tgtagcccgc cttctttttc tcctcggcat cggcgtcctt caccagcccc agcaggatgt
      361 cctccggcag gtccaggctg cgcgccacgc gctggatggc ggtctggcgg gccagcggcg
      421 agtcgaacac gcgcagcagg tccaccacct cgcgggcaaa gcgcacgcgc tcgcgcatct
      481 ccgtcaggtc gcgccgcgcg cctgccgcag tcaggtggtg gtcgagaaag tcgcgcgcgt
      541 ttttcagcag ctccgcgaag gcctccggcc cgcggttgcg gatgaaagtg tccgggtcgt
      601 cccctgcggg caaggcggcg actttgacga tcaacccgtg cggcgccagg atgcggaagg
      661 ccttggtcac cgcatcctgc cccgagttgt ccgagtcgta gcacagcacc acctcgtccg
      721 cctgccgctt cagggcctgc gcgtgatact cggtgaacgc ggtcccctgc cccgccacca
      781 cgttgggaaa gccggcctcg tgcaccatga tcgtgtctat ctgcccctcg cagacgatgg
      841 cctggcccgc cttggaaatg aagcgccgcg ccttgtcgaa gccaaaaagc acccggctct
      901 tgttgaagat cggcgtgtcc gaggtgttca ggtacttcgc cgtcttcgcc tccgggtcga
      961 gcagacggcc ggagaagccg atcacctcgc cgttctcgtt gcggatgggg aacatcagcc
     1021 ggtgccggaa gcgcgggtaa accgcgttgt cctcctcgct ccagcccagc agccccgcct
     1081 cgacgagcaa atcccgcccg aacttgaaag acgccgccca ctggcgcaga agctccagcg
     1141 aggccggcgc gtagccgatc tcccagcgct tggcgatgtc cgacgagatg ccccgcccct
     1201 tcaaatactc ccgtgccggc gccgccagcg ggctgcgcag cagcaggttg tgataccacg
     1261 ccgcgatctc cttgtgcgcc ttgagcatca gcgcgcgctg cttcgccgcc gcctccgcgt
     1321 tcgcgtccca cacctcctcc tgaatgcgga tgcccgccgc ctccgcaagc cgccgcaccg
     1381 cctccatgaa agtcagcccc tcgtgctcca tcagccagcg gaaggccgtg ccgctggccc
     1441 cgcagccgaa gcagtggtag ctgttcgtcg aaggccgcac attgaaggag gggctcttct
     1501 cgttgtgaaa agggcacagc cccacataat tcgaccccgc ccggcgcagc ttcaccgagc
     1561 gcccgatgag gtccacgatg ttcgtggccg ccagcacctg ctgcagagtc tcctcaggga
     1621 tgcgcgccat gatgtgtcac tccttgctca gggcgcgggc agccattggg ccgcgtcaat
     1681 gatgacgtgc cccttcgtgc cctcgttgcc gatctccacc cagcccgcct tgcccgcctc
     1741 aaagtggaag ctgccgagcg gctgaaagtt gttcttgccc gccggcttct gcttttggtt
     1801 caccagcacc ctcacctccc cctccgcgtg gcggatggtc acgggcacgt ttgtggcgcg
     1861 gttgcccagc gcgctgtagg agatggaaac ctcgtacttg cccgccttcg gcagctcggg
     1921 tgtgaagcgc gcgctctgcg cccccttgtc cttgtcactg tcatggcggt agccgcgctc
     1981 cacaaagccc ggcgtcgtgt gcccctcgga gtcgaagccc gtcagccgcg cctccgtgtc
     2041 atccacgatg atgccgggca ggtcggcctt tttgaaaaac gacacttcca ggatcggcgg
     2101 cgactcaaag tccagcacct ggccgtcggc cagcaggcgc gtcttgagct tctcatagct
     2161 gatgtcctgc accgccacgc cctcctcgat ggcctgcacc gccgcgctcg ccgcgctctg
     2221 gcccagcacc atgaacaccg gctccatgcg gatgctgcca aaggcgatgt ggctcgagct
     2281 caggcacacc ggcaccagca ggttcgcgca ctccgaggcg cgcgggcgga tgctgcggta
     2341 gctgatggga tacgggcggc tgcgcacctg cacatccccc tcattgcgca cgaaaccctc
     2401 cttcgtaacg tagcgctgga tgttgtgcga gtccatgttg taggcgccca tgcccacgct
     2461 gtcctccacg atctcctggc ggcggcagtt cttctccgtc atcacataat cactcaccat
     2521 gcgccgcgcc tcgcgcacat agagctggcg gggccagttg tcgttgtcca ggaactcatc
     2581 cctggccagc cccatctttt gaaaagcctc acggatgcgc tccggcacgc gcggatggtg
     2641 cgcgtaagtc cacatgaggc ccttttgcca gtcctcgtgc gctttccata tcttctcccg
     2701 cgtggcgtag tccgcctccg gatagtccca gttcatgccg atgaagtccg tgctgacggc
     2761 gaagttgttg ttcgtgtccg tcttgcgatt cggcatccat gccggcgccc acgagatgcg
     2821 ctcgtccccc gcctccacat tgcgcagcgc cagctcaaac atgcgctcgt catagttcga
     2881 gggcttctcc cacgcgcgtt tgttttccgg gtcgtccgtc gtacacatgc ggaagttgta
     2941 ggcctgcacc ttgcggtcgc cgctgaactc cacgcccggg tcggtctcga tgcccggcag
     3001 caggccgctg gagggatcac ccggcttcac atacgggtcc acgttcacct tgaactggtg
     3061 gtgatggctg tggcccacct gcacgccgtt gagcgtctcg ccatgcacac tgttcgcctc
     3121 ccgccccaca tggtagctca ctcccgcctt cgccatcagg tcgccctcat aggtggcgtc
     3181 aatgaacatg ctgcccgtga aggcgcgccc gctctccatg acaatgcgcg tgatgcgcgc
     3241 cccctccttc accacgccgc ttttcaggtc cagccgctcc ccgcgcacca ctgtcaccct
     3301 gtccttcacc gtgtcgagca tcgcgtcata aatctccgag gcagccttcg gctcgaaggt
     3361 ccacatcgtg tcctcgctgc ccgtgttccc gtgcggcttg cgcgcgaagt aggcctcccg
     3421 cgtctcctgc ttccatttcg ccgggtcgct gtagtattga aagacctttt gataaaactc
     3481 ccgcgacatg ccgccgatgg cgcgcttgtt gccgatgtcc gtggccccca gcccgcccgt
     3541 cgtcagcccg cccaggaact gcgtcggctc gatcaagatg gccgtcttcc cctggcgcgc
     3601 cgtctgaatc gccgccgtga tgccgccgga ggtgccgccg taaacgacca catccgccac
     3661 atccgccaca tccgcggcac cggccagaag ggggaggaaa acgagaaagg ttgggattcg
     3721 catggtcccc gatcaaagcc gggaaagcgc tttgaggaag cgcaaaatca tcccccgcgc
     3781 catccccgga gcagccccgc cgcgcgcgcc gcgcatccgc acccgcgcgc cacttttccc
     3841 tttcccgcaa tactccgccc tgtgaagccc gaattccgca tcttcctctc cggcgtctcc
     3901 agcgagttcg agccagcgcg ccagcttcat tcccagcgcc aaccgcacgc cgacccagtc
     3961 tccatgcctg atctccacgg tggagcgctg ccttgttcgt gcgacatgcc atcgctgcac
     4021 cgcagaaggc tggcatgggg ttcgctttct ggcactggtt ttgagctttg ccaagagccg
     4081 ccggcagtat cttctccgcc ggatgatgat tctggccggg gacttggggg tggcttggga
     4141 tgttccccat gattggcggg tggctttttg tatttttgcc gccaggccag cctggacgcg
     4201 gagggcgggc gcgccgcatt gccgcagcgc gggcttgcac ggcggggcgc gctccggtaa
     4261 aacgcggctg ccatgaacaa ccgccgcgct ttcctcgcca cgctcgcctc ctccgccgcc
     4321 gcttccgcct ttgcccggga ctggaccggg ctggtgccgg agcgttatcc tgacccggat
     4381 gtcatcgcgc tggagccgga gttctccaag ctcatccaag ggaacgcgcc ccttcgccgc
     4441 ctgcacaccg gcatgctctg ggccgagggc cccgcgtgga acggaagcgg caattatctc
     4501 gtctggagcg acatcccgaa caacgcgcaa ctgcgctatc tgcccgagga cggccacgtc
     4561 tccgtgatgc gcaacaactc cggcaacagc aacggcaaca cttttgactg gcagggccgg
     4621 cagatcagct tcgagcacgc caaccggcgc gtggtgcgtt acgagctgga tggcaaagtc
     4681 accgtgctgg cggacagctt cgacggcaaa ccgctcaacg cccccaacga cggcgccgtc
     4741 caccccgacg gcggtctgtg gttcactgac cccggctacg gcagcatgat ggactacgag
     4801 ggcaacaagg gcgagctgca cctcaaggag gccgtctatc gcgtggaccc cgccaccggc
     4861 aagatcgaga aagttaccga cgaactcgcc aagcccaacg gcctgtgctt ttcccccgac
     4921 tacaagaagc tctacgtcgt ggacaccggc gcgccgaaga acatcctcgt ctatgatgtg
     4981 gtggacggcg tcaagctggg gaaggggagg gttttcgcgg tcaatgcggg cgttcttaca
     5041 gatcgcggtc ggtccatagg cgttcgcgaa cgtggcaagc ctgacgctcc cgtgatgtca
     5101 ccgggatcgg acaggcctaa tatttcatgg agtgttgcag atctccaagt gttgccttca
     5161 gaccaaattc cagcagttac tagaaacgca ccaggcccca ctcaagacgg tgtcaaattt
     5221 gacaaagtga tgggccattt ccgcggcggc tccgacggcg tccgctgtga tgtggacggc
     5281 aacgtctggg ccagcgccgg ctgggtgggg gacggctacg atggcgtcca catcttcacc
     5341 cccgagggca agctcatggg ccacatcaag ctgcccgaga ccaccagcaa cctctgcttc
     5401 ggcggcccga agcggaaccg tctcttcatc accgccagcc agagcctcta ctcggtgcat
     5461 gtgaacacgc gcggcgcgca cttctgctga gtccgcatga cgctcgccga aaaacagacc
     5521 gccctcatcg aggacctcac cctcatcgaa aacgcccagg agcgcctcgc cgcgctggcc
     5581 agctacaccg gcggcgtgca tctgcccgag cccgagcgcg cggacgacca gctcgtaccc
     5641 ggctgcgtct cccgcgtctg gctgcacact gccctcggag cggaaggacg cgcccgcctg
     5701 cgctgcgcgg cggactcgcc catggtcgcc gggcttgtgg ccctgctctg ccatctctac
     5761 gatgacgccc cgcccgccga gatcgccgcc gtggagcccg agctgtggcg cgcctgcggc
     5821 tttgagaaaa tgctcagccc cacgcgcctg aacgggctgg cctcgatgcg cgcgcaaatc
     5881 cgcgactggg ccgcgaggca aatcccgctt gccccttccg cgcattgatg cgagaacatg
     5941 cgccccggca cgcgctcgta gttcaacgga tagaatggtg gtctccgaag ccgcagattc
     6001 aggttcgatt cctgacgagc gcaccatgcc ggacgggccg cgctcaatga ccggcgcggg
     6061 atcggccccg gcggctgcct ttcacgcgga gcgcggttat cggcgcgctg caaccgcggc
     6121 attgcctcag cacacggcca gtccggcggc gcgggtctgg cggaaccact cgacataacg
     6181 cggaatgccg ccggcgatgg tggtggaggg cttgaagccg agttcgcgga cggctttgcc
     6241 gacatcggcg gaggtcagcg gcacgtctcc gggctggtcg gggaggagat tgacgcgggc
     6301 ttttttgccg acggcacttt cgatggcggc gatgaggtcg ttgagcgtca cggtctgcga
     6361 gccgccgaga ttgaagatgc cgcagagcgg tccggcgcgg tggttcaacg cgccgatgat
     6421 gccgtccacg atgtcgctga tgtaggtgta gtcgcgcgcg gtggtgccgt cgccgtattt
     6481 gtcaatgggc aggccgtcct cgatgaggcg ggtgaacttt gagatggcga ggtccgggcg
     6541 ctggcgcggg ccatagacgg tgaagaagcg caggcagacg gtgcggatgc cgtggaggtg
     6601 gctgtagttg gagcacatct gctcgccggc catcttggtc atggcatagg ggctgatggt
     6661 ctggaggatg ggatcctcct cgctgaaggg cacctttttg ttcacgccat agaccgagga
     6721 gctgctggcg aagacaaagt gccggcagcc ggtgcggcgg gcgctttcca ggaggtgaaa
     6781 ggtgcccttg atgttcgtgt caatgtagag gccgggctcg gcgatggagg gccggactcc
     6841 ggcgcgcgcg gccaggtgga tgacggcgtc gaagcgcccc tcctcgaaaa cccggctcac
     6901 gaagtcggcg tctgtgaggt cgccctcgcg cagcgtgacg cggccggcga cagcctccag
     6961 attccggcgc ttgatggcgg ggttgtagaa gtcgttgaag gagtccagca cggtgacggt
     7021 gcccgcgcct tcgtccagaa gccgctccac cgtgtgggag ccgatgaacc cggcgccgcc
     7081 ggtgacgagg atgttcatcg gggatcaggc cagggcttcc aggcgtttga tcagggcgtc
     7141 tttggactgc acgccgacca cggtgtccct gacttcgcca ttcttgatga agaccatcat
     7201 tggaatcgag cgcacgttga actgcgcggc caatccggga ttgtgatcca cgttcacctt
     7261 gcccactttg accgcgccgg ctttttcctg cgccaactgg tcgagaatgg ggccgagcat
     7321 cctgcacgga ccgcaccact cagcccagaa atccacgatc acaggcacgt tgctgccgga
     7381 gatttcgctt tgaaaattcg cttcgtcgag tgtgagaacg gattcggagg ccatgatgaa
     7441 aaaaacgggg ttgaggatgc tacgaggcgg gagccgcctt gggacgcatg aggtaggcgc
     7501 ggccttcccc gaatcaatca cttttccgtg cctttgccgt cttttgcccc gcctgccgcc
     7561 gctgccgcgc cgcctcgaag agaatgaccg cgccggcggc ggccacattc agggactcca
     7621 cgccgcgcgc cattgggatg cgcaggcgcg cgtcgcaccg ggccagcagt tccgggcgca
     7681 gaccctggcc ctcgttgccc agcagcagca gcaccgggac acgccagtcg aactcgcagt
     7741 agtccatgtc accctcgccc gtggcggcag ccactttcat gccgggaggc agagtcaggt
     7801 cgccgccctc cgcagccgcc agcggcatgc ggaaggcggc ccccatcgcc gagcgcagaa
     7861 ccttcggcga ataaacatcg gcgcagccgc cgagggggat caccgcatcc actcccgccg
     7921 cctcggccgt gcgcagcagc gtgcccatgt ttccgggatc ctgcacacgg tccagcccga
     7981 gcagcagggt ggcggacggc gtgaccggcc tcagggccgg gcggcgcgcc aggaggatga
     8041 tgccctgcgg agcctgcgtg tcgctgatcg aggcgatcac cggttccgtg acctcgaaga
     8101 caggcgcgcc tttgatccgc ggatcacgaa gcagcgcacc ggcggccgcg tcgcgcccgc
     8161 gcgcgtccac ggtaataaac gccgcctcga ctttgatatc cgacctcagc gcctcggcgg
     8221 caagcttcgc cccttcgatg aagatcatgc cgggttcgcg tccgtccctc acactgcggg
     8281 cggctttgat ccggtcgttg tccttgctgc tgatgaccat cggccagctt gccccagcca
     8341 atccgacctg tccacgcgag agcaggagct gacggccctc gaacgaagaa aaggtgcgcg
     8401 cgcttcaaat gcccttttcc cccagccaga cgctgctgtg cggaggctcc tcgcaggtga
     8461 ggccgaactg ggttgccccg atgtgtacca ggtgcccggc ggcctcctcc acatcatcgg
     8521 tccagagaat caagtccagg tcaccggggc tgatagtgcc gtttttgacc atgtcatcca
     8581 caagaccgcg cagttgcccc cagaattcgc ggcccacgac cacgacggga aaattcctga
     8641 tttttttcgt ctggatgagg gtgagcgcct cgaacatctc atccagagtg ccgaagccgc
     8701 cgggcatgat gacaaaaccg taactgtatt tggtgagcag caccttgcgc acgaagaagt
     8761 acttcatcgt cacccagcgg tgcaggtagg ggttgtggtg ctgctcgaaa ggcagttcga
     8821 tgttgcagcc cacactgcga ccgcccgcct cgtaagcgcc gcggttagcc gcctccatga
     8881 taccgggtcc gccgccggtc atcacggtga aaccgcgccg cgcaatggcc gcacccacct
     8941 cgcgcgcctt ttgataatag ggatggtcct ccttgaaccg tgccgaaccg aacacagtga
     9001 tgcacggtcc cacgaagtgc agcgcgcgga aaccccgcag aaagtcgcgc atcacggcaa
     9061 acaaaagcga aagctccttg aggcggcggt tcggcccctg caaaagcgtc tgatccacca
     9121 agcgcccgca aaaacgccgc tgctcatccc gctgctcagg agtcacttca ggaggggtgg
     9181 gcagcgggcc tgtgcctgag gcacggcctt catcatggtg gcgttgcggt ttggacatgg
     9241 ctgggatttg ccaatggact acgcttttga tggaaagaaa agctccgaat cccatgccaa
     9301 agcggcaaac cgcaacggcc atcttgtccg cccttgtgcc acaatgtcgt gcgagcatcg
     9361 gcgcgagaaa ccccgccaaa gcgcggtgaa tgcttgccaa ttcaaggttg gcgcctccct
     9421 caagcttcat ggcatgcttt aagcaaagac catggcaaaa aaacaaccac ctgaccaacc
     9481 atgtcgcccg aaaaattcac cgtgaagctc caggaagcct ataacgcctc gcaatccatc
     9541 gccacgcggc atgggcacca ggaactgaac cccgcccacc tcctcctcgc cctgctggag
     9601 caggaaggcg gcatcgcgca gccgcttttc cagaaagccg ctgtcaatcc ggaggcggtc
     9661 actgttctca cttcgagcat ctcacaactc ctcgcaaaac aacccaaagt atccggtgcc
     9721 accggcggcc tttacctctc caatgagttc cgcgatctca tgacgaaggc cgaggacgag
     9781 cagaagaagc tcaaggacga gtatttgagc gtcgagcaca tcatcctcgc gcttttcaaa
     9841 acgaagtcgg agctctccaa cgtgctgaaa caagccgggc tgacctacga caccgtttca
     9901 aaggccctca ccgccgtgcg cggcagccag cgcgtggtcg atcaggaccc cgagggcaaa
     9961 taccagacgc tggagaaata cggcaccgat ctcaccgccc gtgcgaaagc cggcaagatc
    10021 gatcctgtca tcggtcgcga tgaggaaatc cgccgcgtca tgcaggtgct cagccgccgc
    10081 acaaagaaca accccgtgct catcggcgag cctggtgtgg gcaagaccgc catcgccgaa
    10141 ggtctcgcgc gccgcattgt tgctggtgac gtgccggact cgctcaaagg caaacgcctc
    10201 atcagcatgg acctcggcgg catgatggcc ggagcgaaat tccgcggtga gttcgaggaa
    10261 cgcctcaaag ccttcctcaa agaggtcaca tcgagcgagg gcgagatcat cctcttcatc
    10321 gacgaactcc acaccatcgt cggcgctggc aaagccgaag gcgcaatgga tgcgggcaat
    10381 cttttgaagc cgcaactcgc ccgcggcgag ctgcgctgcg tcggcgcgac cacgctcgac
    10441 gagtatcgca agcatatcga aaaagacccc gcgctggagc gtcgcttcca gccggtgaaa
    10501 gtcggcgaac cgaccgtcga ggacaccatt gccatcctgc gtggcctcaa agagcgctac
    10561 gaggtgcacc acggcgtacg cattcaggac ggagccatca ttgccgccgc cgcgctgagc
    10621 aatcgctaca tcaccgaccg ctttttgccc gacaaagcca tcgacctcgt cgatgaggcc
    10681 gccagccgca tcaagatcga gctcgactcc atgcccacgg agatcgacgt catcgagcgc
    10741 gacatcatgc agctcgaaat ggagcgtcag gccctcaaga aggaaaaaga tgccgcctca
    10801 aaggcccgcc tcgaaaaact cgagcgcgag atcgccgatc aaaaggagaa atcctccgcg
    10861 ctgaaggcgc agtggatgaa ggaaaaggaa tccgtcgatg ccggtcgcaa ggtgaaggaa
    10921 gaaatcgacc gcctccgcac cgagcaagag caagcccagc gccgtggcga cttcgcccgc
    10981 gccggtgaga ttcagtatgg cttgatcccc aatctcgaga aaaaactcca atctgctcca
    11041 accgagaacc gcgaaccgag aaccgcgaac acgcttttgc gcgaagaagt cactgaagaa
    11101 gacatcgcca aagtcgtcgc caactggact ggtattcccg tttcacgcct tcaggagggc
    11161 gagcgctcca agcttatcaa aatggaggaa cgcctcagcg cccgcgtcat cgggcagaag
    11221 gacgccatcg tcgccgtcag caatgccgtg cgtcgggccc gtgcgggcat tcaggatgaa
    11281 aaccggccga ttggcagctt cctcttcctt gggccgaccg gcgtcggcaa gacggagctt
    11341 tgcaaagcgc tcgccgagtt cctgttcgac gacgacagcg ccatgacgcg catcgacatg
    11401 agcgaataca tggagaagca cagcgtcagc cgtttgatcg gcgcgcctcc cggctacgtc
    11461 ggctatgagg aaggcggcca gcttaccgag gctgtgcgtc gtcgccctta cagcgtcgtc
    11521 ctgttcgacg aggtcgaaaa agcgcatccg gatgtcttca acacactgct ccaggtgctc
    11581 gatgacggcc gcatcaccga cggccagggc cgcacggtcg atttcaagaa cacggtcctc
    11641 atcatgacca gcaacatcgg cagcgccgcc atccaggacg tgagcaacgc cgagcagcgc
    11701 gatgctttgg tgcgcgacag cctgaagcag ttcttccgtc ccgagttcct caaccgcatc
    11761 gacgagatcg tcatcttcga ccgcctcgac gccgcgcaac tcgaccagat cgtcaagatc
    11821 cagctccagc gcgtcatcga ccgtctcgcg aagcaaaaca tccacctcac cgtcaccgac
    11881 gacaccaccc gccacctcgc cgacgccggc ttcgatcccg tcttcggcgc ccgccccctc
    11941 aaacgcgcca tccaaaagca cctcctcgat ccgctgagct tggccgtcct tgatggcggc
    12001 ttcaaagaag gcgatcacat caccgcgacg atgagtggcg ggaaggtggt gtttgagaag
    12061 atgtgatgga gtgctgatct tcgctggtga taggggagca tagattcgat tggcaatcga
    12121 actccgctcc cctaaactac agccatgacc gaagccgaac gtgaactcac caaacgctgg
    12181 gttgactcct gggcgaaagc tgctcccgag ctgcaaaaag tgcgggatgc ggacatccgc
    12241 gcggcggaca cagctggatc gatgaaggtc tttaccggtt ccgccacctg ggccgtcaag
    12301 aacagacccg ctgcggcatg gtctgggctc gttgagcagc agcgtttgtt tgccaaagcc
    12361 gccctggcat catgaacgaa ctcaccaaac tcgccctgga attacaaagt ttttgtgaag
    12421 agcggaaatg gtccttttgc atcataggcg gcattgccgt gcagcattgg ggtgagcctc
    12481 gtttcaccaa ggatgtggac atcacacttc tcaccggttt tggcagtgag gaacgttttg
    12541 tggacgactt cctcgccgct tacgaggcgc gggtcactga tgctcgtgcc tttgcccttc
    12601 aaaatcgcgt gatgcttctt cgttcttctc aaggcatcgg catcgacatc gcccttggtg
    12661 cgctgccttt tgaagatgag gcggtgaagc gtgcaatgaa gatcgaaatg gaacccggag
    12721 ccagtctgcg gctctgcacc gccgaagacc tcctcgtcat gaaggctttc gccagccgac
    12781 cgctcgattg gaatgacgtg cgcggcatcc ttgtccgcca gggcacgcga cagctcgact
    12841 ggaaatacat ctatctccag cttcagcccc tctgcgaagt caaagaagcc cctgaaatcc
    12901 tgacccagct cgaaaacctc cgtctagagg tcgctcaaag cgagccgtga aggtatttgc
    12961 aggcgctcca gcgcgtcacc gaccgcctcg cgaagcagaa catccaccta ctcgccaccg
    13021 acgacaccac ccgccacctc gccgcccccg ccttcggcgc ccgcccggtg aagcaaacca
    13081 tccaaaagca ccttctcgat ccgctctccc tcgccttcct tgatggcagc ttcaaatacg
    13141 gcgatcacac tcccgcgacg ctgaagatcg ggaaggtgga gtttgagaag aggtggcttg
    13201 atcctgcacg cggatacgcg agtggctcga tattttccaa tcggctccat ccagcgccta
    13261 tcacatgtag gacacaagaa cagtttttcg ccattttgtc taagtattaa cggtctaata
    13321 ttagacaaga tcaaaacttt ttgagtcata atgttgacac agtgagcatt attgccattt
    13381 gtctagccgt ctggatagtc attgtttcga acaatcaaac cacctccact ctttatgaca
    13441 gccacacctg acctcgaagc acagcagcag caaatcgaag aggacatcaa actcctggag
    13501 cgcatttcca acagggatgc ggcggcgttt caaacctttt acagcaagta ttccggcctc
    13561 atttttgccg ccgtctccaa tgtcctcaat gaccatcacg acacggagga tgtgatgcag
    13621 gaggttctcg tccagctttg gaacaaggcg catctctacg agccgcgcaa aggcaagccg
    13681 ctgacctggt tgaccaccct ggcacgaaac cgcgccattg accgcatccg ctccaagcag
    13741 cggcgcgccc gcctcaatga tgacttcgag atggagaaca aaaagctaca atttgagttt
    13801 gaagaatcag gaagcgaggc gctggaaatc aaggaaaggg atcgtgtcct gctcagcgcc
    13861 gtggagcgtc tcaatgacga gcagcgcgaa gcaatacggc tggcttattt cgatggcctg
    13921 acccaggccg aggtggccga gcgcctcaac gagcccctgg gaaccatcaa agcccgcatc
    13981 cgcagggggg tcaaccgtct cgaatccctg gtgagacccc gccttgcctg agcatgaggc
    14041 caatcatgcg cgctgggaca tgccaaggct gcctgagagc cagtcgcggg ccaggagcgc
    14101 cagtccgatg ggaagtccgg ccagccacaa agcgcgcgcg cgcacggctg gcagatccct
    14161 gacctgggaa agcgggcctt taccgctgag aaaactgaat cgcacatact gaccggctcc
    14221 gcgcagcagc attaaaggcg cgtcacacat ataaccggac tcgaattcca ggattttttg
    14281 gaacatcaac ctgtggccgg cggcattgcg gcacggattc cgcatgccgt ggactagcga
    14341 tggcgcatcc atccaataaa tcctcagcat gtcgttgaaa tgccgcgttt tgtagcgccg
    14401 cgccacctgc gaccagatga acgattcagg aataaaatct cccggaacat tatccggata
    14461 gggaaactgg cgaagcacat cgaggcgcaa gcatccccat ttttcacctt cgacccggtg
    14521 aatgtattgc agctcggcgg aagtgcagtc gagagggtcc agcggaaatc gtccgcccac
    14581 cagccggccg tcctggtcct cacaaaggcc ggtcacgccc gcgaaaccct cgcgctcgtt
    14641 ctccgggatg ctttgccaga catgccacat gcgcccgagc gcctcgggtt tgcaggcgtc
    14701 atcggagtcc agctttaccc aaaagcgacc gcgcgcggcc tccaggcaga aattgtggac
    14761 atgatgcgcg ccaagatgcg atcgctgctc gtagcgaatg gacacccttc cttccgcctg
    14821 ccagcccgcg accaaatcag ccgtgccatc cgtggagcca tcgtccacga tcaaccactc
    14881 aaagtcgcgc aaagtctgcg ctttcaagct ctcatgacag cggtgcagcg tgtgcgcgcg
    14941 gttataagtg ggtgtgaaaa ccgtgaaaag cggtgatgtc atgaaagttg aagactgtga
    15001 aatatagcca atcaaacagc caaaagcatc aaagtttctt tattaaactc caagccaaac
    15061 ctttgcgcag cagggcaaga gtatggacac cgaagcagat taccgcgttc agcgctagac
    15121 gggcagccct ccgccgttcc cagcagacat gaatcccgcc cccccttgga tctcggtaat
    15181 cgcgtggcta agtctggtgc cggcagcctt tgcagtgcca gcgcagcaga agccggatat
    15241 gctcgtcatc ctcgcggatc aatggagccc gcgctacacc ggctgggaca accctcaggt
    15301 gcgcacaccg cacatggacc gaatcgcggc tgaggggatg atttttgacg cctgccacac
    15361 cacctctccc gtgtgcatgc ccgcgcgagt ctcgctgatc actggcctgt atccgcacaa
    15421 cggaggccac gcgctgtggg gaaatggcgc gcagttctat cccaagccgg aggacgcgcc
    15481 aatgtttctc gacattcagc gcgccggcct gacgacggcg cagatcggca agacacactg
    15541 gacggcgggc ccggcgtggc atgagcattt caaaaacagc ggcgccttct tcaaagcgct
    15601 cggcctcgat catgtggcgg acattcccgg accgcctgac tccgtcaacg gacgcgatcc
    15661 ctactcccgg catctgcgcg aacgcggact gctccaggcg gtggctgatg atttgcacgg
    15721 gcgctacatc agaggcgagt acgagccgcg ggcgagcgtg gtgaagcctg aggattatca
    15781 cgatgtgttc accacggacc tcgcggcggc tttcatccgc gcgcaaccgc aggacaagcc
    15841 gctctgcctc gtggtcagtc tgcactcgcc gcatccaccg cttgacgcgc cgggtgacta
    15901 cgccgcgatg ttcgatcccg aaaagctcac gctgcccccc agcgtgcctg agaggtatct
    15961 gcgcgaggga cgcgccatgg accatgcgaa gaccaggcgc atgctggcga actacctcgg
    16021 caaactcgcg ctcgtggacg actgcgtggc aaagctcgtc gaggccctga aagcgcgcgg
    16081 cacctgggac ggagccttcg tcgccatcag cgccgatcat ggcgagatga tgggcgcgca
    16141 tggttatctc accaaaggcc gcttttacga cgagtccgtg cgcgtgccac tcgtgctgcg
    16201 ctggcccgcc caggtcaaac ccggccgcag caaggccccg gtgcagatga tggacatcta
    16261 tccgaccctc gtcgaagccg ccggcggcga actgacgccg ggacgcttcg cgaaatccct
    16321 tctgcccatc gcccgtggcg aaaaagaccg cgtccgtccc atcgccatca gcgagatcgg
    16381 tgacaaagcc ccgctgcgca tcatggcccg tgacgaccgc ttcaaatact gggccgacga
    16441 ggagcgcgag tacctcttcg acctcgaaag cgacccgctg gaaatgcacg acctctccac
    16501 cgcgccggat catcgtgaaa cactccacgc catgcgcgaa aagctgctca cacacctccg
    16561 ttccacacag tcaaacctct ccgcgggcta caagcccaaa gtccagcgtc tgcgcgaggc
    16621 cgaagccaga aaagccgcgt ccggtgcgaa gccctgaaag cacgcctgat acccgctccc
    16681 ccatccccct cttgccgctg aaaaggctcc aatagaattt cagcttgcac aaaaggacga
    16741 cgaatttcgc caagtcgctg gaactcgtcg ccatgcctgg ctggcgcaat tccatagccg
    16801 tggtgattca atcattatgg tttgtcgagt tgcgcgcgta atccgcgcag aaactcggtc
    16861 aacgggtcca gacccggagg cggcggggca agcttggcga gttcggcgag gattgcctcc
    16921 gctttgtcga gatgcgcggc agcagaggcc ttcagcgcgg ctgtccgccc ggacggactg
    16981 gaagggatgc gggccagggc ggtctccgcc agattgagat gcacggcgga ccgagcgtgc
    17041 cactccgccg gcttgtcgcc ggcggaggca ttggcggtgg caggaaggag gatttcgaga
    17101 gcctgctgat aataattttc cgcctcgtca aggcgtccca tctgaaaatg cagcgcgcca
    17161 gcctcattca gcgcctccac cactgaaagc cctgcggatg aagcctcctc gggagaggca
    17221 tttttgcggg cctccagagc aaagctcaac cactgctgaa gaactcgcac tgcgctgtca
    17281 tttttacccg cgccgctttc ccaggcggcg agtgtctttg cgagtttggc cagaattgca
    17341 ttttcgccag gtttggcatc agcaatgacg gaagcgctga gacaatcatg tgcggtctga
    17401 agcgccgcct cagcctgccc agtagtgtgc tgaagtgccg cggcaaggaa tccagcacca
    17461 agccagagat agcgggaatg gagagtgccg tccctgggtt gagattgata gacgtgcaac
    17521 gtctccctca aggcgtcaga gatgctgccc ctggtcagcc ggggacgcca gaaatcggtg
    17581 agttccgcca gaactccggc tagtcctgcg gcgcgatagg ccttttcctg gctatcgaga
    17641 gcttctgtga tggggatgct ttgcagcacg agttcggcaa gttgggaagc atgattgccg
    17701 cgaatctgat caagcagcag gctctgatcc tcgcggcccg caaagtttct ttgggttatc
    17761 tcgatcaatt tttccagagc atcggcagtg tctttgaatt tgggatgcgc ttgaagtttt
    17821 ggcagccagt cgcgggcttg ttgcagcagc ttccctgcgg atgtgaagtc gccggatttt
    17881 gctttcaggt cggccaactg ctgcatttga aaaacaagag cctggacccg gcttgggtga
    17941 tcccggctgc ggagcgtcat ggagcgcatc aactccaggc tgtcttcgag ccaggaaagt
    18001 gctttggcgg cgtcacccga ctcaaagtcg aatttggcgg cggcgcccat gcagttgatc
    18061 atgaggtagt tcgcataatc gtgctcgggc agtcgctgga gatactggcg ggcgagttcc
    18121 gtggcttcgg cgaccagatc ctttctctgg ctcaatgccg ggccggtctt caaccgctct
    18181 cccgcgctgc gcaggtcatc ggcgagggcg agcaaagcgt agctgagctg ggaaaggtaa
    18241 tcgttgttgt tcggctcaag ctcgcgcgcc ttgcggtagt gctcgcgggc tttgctccgg
    18301 tgatcgaaaa tcatgcgtga caattcctcg atttgaggcc ggatctccgc gagagccttc
    18361 tcgtagtcct ggatgcgtcc cgcctgaaag tcatttgccg cttgtgagag cagggcgctg
    18421 gcacgggcgg agacgatctc ggcgcgctgg ttttgctgtc cgccgagcag ggactgccag
    18481 cgcgcttcac cgagtgcggg accggctgct tggaagagcg cctcccggcg ctccagcaga
    18541 gcaagacgct cgtccgcctg atccccgcta tgcggcatag gggcaagacc gctaatggcg
    18601 cggcagagac ggtaaagcgc ctcctcatca tccggtttgg tcttgagcag ttcctcggcc
    18661 atctccacct gccggcgcgc ctgaagcatg gcccgctcct tgtcgccgga gttctgcagg
    18721 atgagggcgg tgctctcatt gaggtcaaga cgcacgatga catcctcggc gcgccgccct
    18781 ccatgcagcg gaaccgtatc catgtagttc ttgagattgg cggcgatctt ttcggccatg
    18841 tcgaagcggc cggtttcatt ggccagttgc aagacatcct tggatagcag cgcccccagc
    18901 agacgttcgg cggccagacg atccgtccgg gcgcgctcgc gctcggcaaa ccacactgcg
    18961 gcgatggcgc cgacaatccc aagaagcgcc agtgaggaga gtgtgagcgc aacgcgcctg
    19021 cggttcttgt tccgggcctg ctggaaactg cggctggaca gttcgatgaa ctcgctcaat
    19081 tcggagttga gcagaacggg cgcggtggcg atgagccggc agccttcctc caggggcagg
    19141 ccgggcggca gcagcaggct gggatttcgc ccgccctccg cccagcgcgc atgagcctgc
    19201 tcgactcggg agcgcaggcg caggaggtgg tggttttcac gcacccaggc ggcgaggtcc
    19261 ccccaggcgc cgagcagggc ctcgtgggcg agggtgagcg tggggcgctc cgagtcgcgt
    19321 ccggtggtca gcaaacgcgc cagcacaagg gctgcggcga ggcgtccccc ggggctggag
    19381 acatcatcgg gatcataaac ggaggcgcgg cgcaccggct ggcgctcctg gaggggatca
    19441 agcgccacca gccgctggaa gagcgcggga agagcggcgc gcacatcggc gggcagggag
    19501 ttaatgacgc tgcccgcgcg cctgcccgcc gctccagcga tgccgcccat gtcgcggtag
    19561 gctgcgaagg tgagggtgcg gttctggcgg cgttcgtaaa gctcgtgcag ggagaattcc
    19621 agcagcggca gcgcgtcggg gtggcggctg gcttcgtcga gcaaaagctg gtccagcgtg
    19681 gctccggtgc gctcgtcctt ctcaaagaca agtccggcga ggcgcgcggg cgcggtgatc
    19741 atgcggcgca tggcggaggg cgcgggcggg ggcaagtcaa actggccgcc cgcgcccttg
    19801 agttcgagga aggcggggtc gcgctggagc gcgccgtaca agtcgctgcg caaagtggcc
    19861 gcgatccaga agcgcccgct tgcggccaga acctggaggc agcggaagaa ggcatcgcgc
    19921 gtggcgggtc caaagcgggg gtcagtgtgc gtctcctcaa gctggtccac gatgagcagc
    19981 agcttgatgt gaccgccgca ggcgcgcgcg gcgcggtcca gcgcgggcag gagcgccaga
    20041 cgcagggcgc tgcccgggga ctctgcaaaa gcggcggcca gagactccgg cgtggttccg
    20101 ttttcgcgcg cctccggcag gcatgaaccg gagcacagaa cacgcgccag accgagaaga
    20161 agatcgccct cgcaccggac gggagtgagc acggccacgc gccattgcgc cacgctttca
    20221 tcaaggttga agcgagcaag actggccgcc aggcccgcgc gcaccaggga ggacttgccg
    20281 ctgccgcttg gtccgacgac gacgacaaaa gcctgctccg gcttggagcg gagcatgtcc
    20341 tccaagtcgg caagttcccg ctcacggcca tgaaaaatct cggcatgggc gggttcgaag
    20401 acatcgaagg ccagatacgg gctgccttcc caaaggcgcg cgcccgccag ctcttcccca
    20461 agcatcgtgt cgagaaactc gcgcaaatga acgcgcagcc ggctggcgaa ggtgagcggc
    20521 tccgcgaagg tgtggtaggc gcgcaggttg gcgcgggttt cgggatcatg aaacacctcg
    20581 cgcacgaatt cctgggccag aagctgctgc tgaatttcct cgggcaggcg ctccgcaggc
    20641 atctcctgga gccggcgttt gaacccggcc tcatcctgcc gcagataggc caggatgtgc
    20701 ggacgcctgc cgccggactg ccgccgcgcc tccatcatca tatcccactc gcgctccgtg
    20761 ccactgcggt agcctgatcc gtctgagcgt gaaatgcgcg ctcccagagg ggagccgagg
    20821 cgcgaccata aaatgaaaac agccaggtca atgccgccgc caccggaaag gatgtggtct
    20881 atgccctcct gaaaggacag gtccgcgccg agaggcaggt cctcccacaa cacgggaacc
    20941 agcttcaccg ctccatcata acgccgctcc aactgggtga tgacctggcg cgccttttca
    21001 cgttcggcgc tgacatcgcc cggactggag atgaagatgc ggatggtctt gctcatgatg
    21061 actggcccgt tgcgtgtttt tgccagccgc cacgatcatc ctcaccggcg tagcgcagcc
    21121 tgagcgggga gacggcgccc gcgcaagaaa aaacttcaaa gcaaagcgtc acgcaccacc
    21181 ctggcctcct cgacgcctgt gaggcggaag tcgaggccct gggcgcggta ggtgaggcgc
    21241 tcgtggtcca cgcccagcag atgcagcgcg gtggcgtgca ggtcgcggac gtggacgggg
    21301 ttttccacaa cgttgtagct gaagtcatcc gtggccccgt aagtgatgcc cggcttcacg
    21361 ccgccgccgg ccatccagag ggtgaagcag cgcggatggt ggtcgcgccc agcctcgggg
    21421 ctgtcccact ggccctggcc ggccacgccg cgcccgaact cgccacccca gatgacgagc
    21481 gtgtcatcaa gcatgccgcg ctgcttgagg tctttgacca gcgcggcgct gggctggtcc
    21541 gtgtcccggc agcgggcggt acaccagctc ggcaggcggc tgtgatggtc ccagtcgggg
    21601 tggaagagct ggatgaagcg cacgccgcgc tcggccagcc ggcgggccag gacacagttg
    21661 gccccgtagc tgccggggcg gcgggagtcg gggccgtaga gttcaaaggt ggagtccggc
    21721 tcgtcggaga gatcggtcag ctcgggcacg ctggcctgca tgcggaaggc catctcgtat
    21781 tggcggatgc gggtgaggat ttcgggatcg cccgctgact cgaagcgcat ctggttcagc
    21841 tcgccgaggc cgtcgagcat gccgcgccgc agctcgcgcg gcatgccgtc cgggtcgcgc
    21901 aggtagagga cggggtcttt ggagttgcgc agcttcacgc cctggtggcg ggagggcagg
    21961 aagccgctgc cccagtagtg gtcgtagagc ggctggtcgc tggggcggag catcttggag
    22021 atgagtacga ggtagtcggg caggttctgg ttcacgctgc ccaggccgta gctgagccac
    22081 gcgcccatgc ttgggcggcc cgcgagctgg tggccggtga ggaacttggt catggccggc
    22141 gcgtgattga tctggtccgt ggtcatggac ttgatgaagc agacgtcatc cgccacccgg
    22201 ccgaggtgcg gcagccattc gctgatcgcg gccccgctgc gtccgtgctg aaaaaagcgg
    22261 gtgatgcccg gcaggatgag ctgtttttga ttcgccgtca tcgtggtcag gcgctggccc
    22321 tggcgcacgc tctccggcag ctcggtgccg gcccatttgc gcaggccggg cttgtggtcg
    22381 aaaagctcaa gctgcgaggg gccgccggac tgggtgagaa aaatcacgcg cctggctttt
    22441 ggcgccaggc gggagacgcc cgcgccgccg gcctcgcgcg ccagcagcga ggccagcgcc
    22501 atgccgccga gcgacacaca agcgctcctg cccagaaagc agcgcctggt ttcctgcggg
    22561 aactccgtgc gtgggaatgt tcggctcatg cggaacaatg cgcgctgccg cgcaaagcgc
    22621 aaagcccgcg ctcgcgcttt gcgatggatt atcccgcgcc cggcaccgcc ggcgccccgc
    22681 cctcaccgcg cttctacggg cgtggtgagg aagcggacat ggatggcgtc gtcgggctgg
    22741 taggtgccgg tgacgctggt gccgaagtag cgggaccagg tttcctccga gggagcggca
    22801 tcagggtcaa agatgccgtc cacagcgtct tcggcgcttt tggccgcgcc gcctttgggg
    22861 cctttggcct tggtgctgcc gccaaggccg ctccgcgcca tgacgatggt gtccacgagc
    22921 agtttcatga tgcgcgcgcc gtcggtgacg ccgacgccgt tgtagcctgg cgagagacgg
    22981 gacatgaggt ctttggcgcg ggcgtcctcc cacaggctgg ggccggaggg gtccctcatg
    23041 cgggcgagca catcctggag cagcgcgacg gggccggtgc tcatgagcag gtgggtgtcg
    23101 gtgacgcaga aggcaaagcc ggggctgccg gcggtggcgc cggcggtttt gacggactgg
    23161 atggtgtggc cgagatactc ggtctcatcg aaggcgccga agccggcccc gacgattttt
    23221 ttgacggcct cgaccgcgcc gccgaaacgt ttgccgtcct tgatcttgat ggcgatgacc
    23281 tggctggtga cgccgtccgc ggcgtcggtg atctcgtaat actggtcgtc aaggctggcg
    23341 aagaggtcgt cgcggatttt gacgccgaac tgctgctcgt agatgccgag ctggccggtg
    23401 agcagggcgg cggcgggccc cattttttgc acgatggcga gcagcttgtc ccacagttcc
    23461 aggaggctca tgcgcatggc gctggcgctg accacatccc ccggcacgaa gccgggcagc
    23521 tccactgcgc cagcgccgcc gcgaacgagg ctgagcaggc cggcgggctt gctggcgtgg
    23581 aggacggcga ggtcgaagac ggactgcccg tcttcgaggt tcatcatgag ggccagggac
    23641 tggagttcct ggaggccgag ggcctcgatg acctggtcgg gtgtgacggg cagcgggctg
    23701 cctttggccg ccgtggctcc gaactcgcgc gcggcggtga cggcccattg cataaggacc
    23761 tcgccattga aatagacgag agcgtcgggg ctgccaccgt gcagggcctc gatgcggccg
    23821 atgtgaccga ggatggcctc ggggttctcg gcggcgccgt ttttcagggc caggatcatc
    23881 tgctccatca gggcggcgga gttcgactcg acgagcacgc caccgacaaa ggcgtggccg
    23941 ctctcccaga gcgcaccggg ttcctcggac ttggcgagga tctcgacctg ctccccggcg
    24001 atctcctcgg tgcgggcttt gacgccatcc tccttggcga gcgcgagttc cttctcccgt
    24061 gtcatggatt cctccagctc cttgcgcttg tcccctgtct cgctgaaggc gatgaatgcg
    24121 ggcacgccgt cctgctcctc gatctgctgg ggggagtcgg cgacaaaggc ggccaggctg
    24181 gcgccggggt agcgcgccag attgtcatgc aggctctcgc cgaagttctc cctggcgaag
    24241 gtgtcccacg gagcctcgcc gtccttcatc atgggagcgg tccagcgttt gaaggcctcg
    24301 tcctgcatca tgcggccgat gccgcttttc tcccagtcgg ccatgagttc gggggagtct
    24361 ttgacgctga tgacggcgat ggtgctgggc ggcaggagtt tcagccagtc ctccaggaca
    24421 ccggctcgga cggggtggat ggtggaaagt gcgagggcca gggcggccag ggcggatttg
    24481 ggcagggcgg gtttcatggg ttttgagggg tgattacagc gggcttgtct ccaggcgcgg
    24541 gcgggcgcaa gtaggtgaag ccgctgttgg ggttgcgcgg gtcgaaggcg aaggcgtcgc
    24601 ggtttttgag gaagagcttg agcaggttcg ccgggatgga gaagccgagg ccctcgacgc
    24661 cgacaccggc gagcttcatg ttgttcacgc cgacgacctc gccgcgggcg ttgaagagcg
    24721 ggccgccgga gttgccggga ttgatctgcg tggtgctctg gatggaccac gcgccgcgcg
    24781 tgtcgcgcgc gcgcacgctg acgatgccct gggagacgct gcgctcaagg ccgagcgggc
    24841 tgccgatggc aaagacctgc tgcccctggg tgagcgagtc gctgtcgccg atgggcacga
    24901 aaggcagggt ctccgccgcc gggtcatcta tctggaggat ggcgaggtcc aggaatccgt
    24961 tcatcgcgat gatgcgcacc ttcggaaaga cgcgcttctc caggccgccg tccttctgct
    25021 catagacgac gatggtgatc tcccgctcgc cggaaatgac gtgctggttg gtgatgacgt
    25081 ggccgctctt gttaatgatg aagccggagc cgagaccgga ggcggtctgc acctgcacca
    25141 cggcgccgcc gacgcggtgg acgtttttgt ccacgggcag cggctcgcgc gcgggttcga
    25201 cgaaatagac atcctcggca aggatgctgt ccgccttggg gccggccttt tcctccccgg
    25261 tcttctcgac cgacaccacc tccgcgcgcg ggagggtcag cacctggaag ccgagatcaa
    25321 acaaaaggct gtcgtcccgc tcgcgcagga cttcgccctg gaggacggcg ccgtttttca
    25381 atcggaggat cgaagtttcc gccacgaccg tctggcagac ggcggcgcag aatccggcaa
    25441 gcaatggagt ccagtttttg atcacgaacg caaaatctac cggtccggcg gcaagatacg
    25501 agagttaatt gccacaaaag ggcaaaaaag cctccagcgc atctttggaa acccgctttt
    25561 tggggaacag gtgtaccgcc tgtttgcgca ggcaggacgc ctgcgcccca tgtcacccgc
    25621 gctaacttta atcccccgtc cgctcccccg cgcgcatttt ctggaacggc acgctctcgc
    25681 agacggcgag gatgagatcc tggaacctgt agccggcggc cttggccttg ccggtgatct
    25741 cctgcacggc gggcttgtca ctgtaggcga ggccgcgccc gagggcgtag gtgagcaggt
    25801 tttcggcgag gttgcgggcg aactcgtccg gcatgtcgcg ggcgaggatg ccgcgcagct
    25861 cggcgaggtc tttgaactcc tggccgcgca cgagtttgcc ggaggcgtcc acggggtggt
    25921 tcttgtcctt gtcccgccag cgcccgacgg cgtcgtagtg ctcgaaggcg aagcccatcg
    25981 ggtcgagaaa ggcgtggcag cccgcgcagg aggtgtgctc gcggtgctgg gcgaactgct
    26041 cgcgcagcgt caggttcgag cggcgcacct tgccttcgtc gagagggggc acgccgcctg
    26101 gcgcgggcgg cggcggggtg ccgaggatgt tttccagcag gtatttgccg cgcttcacag
    26161 gggaggtgcg cgtctggccg gaggtgaggg tgaggatgct gccatgcgtt aggatgccac
    26221 cgcgcggggt gcctttcaga gagacttttt caaatttgtc tcccttcacg ccgccgatgc
    26281 cataccattt ggagagcttc tcgttgagga aagtgtagtc ggcgtcgagg aactcggtga
    26341 cgggcctgtt ctccctgagg atgtgctcga agaatgcctg gctttcgcgc ttcatgctgg
    26401 aggcgatgtc gccgcgccag ccgggaaagg cgcgcgtgtc gggcgccacg atgtccatgt
    26461 cccgcagttg cagccactgg cccgcgaagt tttcggtgaa ggcggcgccc ttccactcgc
    26521 cggtcatgcg ctggatttgc gcgcgcaggt ttgcgcgcag ctcccccttc ccggccagct
    26581 ccagcagctt gtcatccggc ggcgcggacc agaggaaata cgcgaggcgg ttcgccaggc
    26641 tgaactcgtc aatgaacgcg gcgccgtccg ccaccgcgcc agccggctga ggcgcggagc
    26701 ggaagatgaa ggccggggag gcgagcacgc cctccagcac gaagcgcagc gcgcccagcg
    26761 gcggctcacc ctcccgcatg gccagcacgg cgaagtcctg ccacggcttg acctcctgcg
    26821 gctgcggcgg cctgcggaaa agccgcgcgg tcattttctc caccagggcg ggcacgtcct
    26881 cagcgcgcac tccggccggc tcggacagcg gcccctcgat gcggaactgg tggacccaca
    26941 ggttgcgatc ctcgcgagtc tcggcgttgt agaagtcgtt gaggaaccag atttgcagct
    27001 cgtgcgcgcc ctccggcagg tcggcctcga aggtgaaaaa ctgcggcgcc tggttcttcg
    27061 ccgtcactga gaacgcgccg atctttttgc ccgccacgcg catgtcgaat ttggcaggct
    27121 cgtcccccgc ctgctgcgcg ccggctttca ccgtgagctt gtatttgccc gccttggcga
    27181 gatggagcgg atgccgcaca tagccgctgc tggccaggag gatgccgccc gtgtcgcggg
    27241 aggaggcgcc ctcgcccttg tcgaagtcct cccccgtcag ttgcatcgcc gggcggccgg
    27301 cgggcttgtt gtccagcagc cagcggacaa agcggctgcc cttcggcgcc agcaggccgt
    27361 gcgggccttt gagcgtcatg cccgccagca ccacgcggcg ctcgccgggt ttttcgccct
    27421 ccctggcggg ctcggcgcgc ggattgagca gggacagcgc cagcttcgtg tcccccgcct
    27481 tgagatgcac ggtctggcgc acggtctgcc aggggccatc ttcgtccttc cagccggtgg
    27541 tcacatccag cgtggtgatt tccttgccgt tggctttgag cgccagcttg gcgggctctc
    27601 cccccgcccg cacggcggcc agttgcagcg tcaaggtgta gtcgccctcg gcggggggat
    27661 acacatggtg ggcgatctcc gactgcccgg aaaaccagcg caggtcgccc tgctcgcgcg
    27721 tgtcgccctt gctgcggtaa aagcgccgtc cgctcacctc caagtccagg tggtcgctga
    27781 cccgcacctc catcgcgtcc gcggccacgc cgcgcgccgc gcgcaggtac ttctccatga
    27841 gcatggggga aaggctgagc acgtcgccga tgttgtcgta gccgtagccg gtgtcatccg
    27901 gcgggaactc gcgcgcgggc cgcgtgtcca caaagagcag gtcgcgcacc gtgttgttgt
    27961 actcgttgcg gttcagccgg cgcagtgtca catggccggg gtcgggccgc gcggggtcga
    28021 accagaagac cgcgtcgtca atccaggcca ccatcgcatc ccgctcctcg atcactggcg
    28081 cgggtttttt ctccggcggc atcacatggg tgacgagctg ctggcgcaca tggtcccata
    28141 atttgaagtc gctccgcagc gccgcgtagt ccttgtgctc gtcaaaggtg aagtcgccct
    28201 tgtccacgcc atcggcatgg cagtcgaagc aatactgctc cagcagcgga cggattttct
    28261 tctcgaactc ctcctggccg cgctcggcag cgcgcgcggc gcaggggaga gcggcgatga
    28321 gcaaaaaatg caggtgacgt gggaacatga gtgcgggggt gtccggcgcg gcgcgccatg
    28381 tacggatacg cacgccggtg cgttctgttt tccccgctgt caacgggctt cagggagagg
    28441 agcttgccaa atcccccacc cctccagtac ttggcaaccg ccgcactttg cgcgccccta
    28501 tccgggaaaa ctcgcgactt ccgctgcgcg cgcccgcact ccgcattcca aaactcgcaa
    28561 tctccgccgc ctcgacacca cggcccaatg catttgagcc ggcctaaaag agcggcgcca
    28621 cccgcttaat tgtcgaagac gatcttgatc ctgcccgcct gcctgcctgc ggcggcgagg
    28681 ctgtcgctca aagtggggcc tgcaagtgcc ggcgcggcga cggcctgctc cgcatcaagc
    28741 gcggctttca gcgcgccttc gaccatctgg ccgcagtgga tcttcatggg cggcagagca
    28801 ccaagcggcg cgctgagctc ctcggcggac atcttcagtg cctcctcctt cgttttgccc
    28861 cggatcaatt cggtggccat gctggcgacc gcgatggccg tttggcagcc gaaactctga
    28921 aaggtggcct tgtcaatcac cttgcgcccg tccttctccg tgaacttcag ccacatgcgc
    28981 accatgtccc cgcaatccgg actgccgacg gtgcccacgg cgtctgcgtc cggcatctcc
    29041 ccgaggttct gcgggttttc gagggcggac tggagggcgg tttcgtacat tcgtcgaatt
    29101 acgcgcaaaa tggcggaatg ctcgcttttt tctgctcttt atcagatcga aaaaatcttc
    29161 ccgacatttc cctccctttc tgatggcatc cactttaatt cgacagtgcg atgtcaaaat
    29221 caacacactc tcagccactc agccagcacg agcacggcgg gcatgttttg ccacctgcgc
    29281 gaagctgtgc ttgtcttcgg tgatcagcgc aactcgccaa gtcgcagccg cttttccagt
    29341 ctggtggcgc cctcctggtc cacgacgcgc agagtcagca gcgggtcggg cgcgtcccag
    29401 tccacttcga gcgcgccggc gttcggctgg tgaaaggtgg cgggcaggct gcggttggcg
    29461 ttgggcgtgg gcgtgccgcg cggatgcgcc tgcgtcatcg aactggcggt gagatcgtgc
    29521 agaggatagg ggacgtttcc cgtcagtgtt gacaactcgc accaatgcct gtcgccgctg
    29581 aaaaaaagca cgccttgcgc gcgggtgacg cggatgaggt ccgccatgcg gcgctgttcg
    29641 agggggaaat tcgcccagct ctcactgcct gaagcggcgg cggcgaattg gatgctggag
    29701 atgacgaggc ggaggcgggc gggctttcgc agttccgcct ccagccagcg ccattgctcc
    29761 gcgccgagaa gggtggcgtc cgggtcgtcg ttggggacgg cgctgccgcc gatgccggcc
    29821 tgctccttcg gcacgcgctt gagcggggtg cgatgatagc gcgtgtcgag caggatgacc
    29881 tgcgtgcttt tctccgcagg tccgaagacg cgggaatggt aaacaccctc ccgcgcccgc
    29941 aggggcgagc cggcgggggt gccgagccag tcatgaaact ctttctgcgc ctccttgcgc
    30001 atcgggtagt cggcgccgcc gtcgttgaca ccgtagtcgt ggtcgtccca ggtggccagc
    30061 acgagcgcgc gcgccagcaa tccctgatgg aaacggctcc ctgccagggc gctgtatttg
    30121 gcgcgcatca cggccatgtc ccgcgtgtcg gcgtagatgt tgtcccccat gtaaatgaac
    30181 aggtccatcg gcagcgcgag cgcgcggtcg agcatggggt gctccacttt gtccaggcag
    30241 gagccgaaga cgatgtggcg cagcggcttg tcatcagcgg cggtggtgat gatggcgggc
    30301 agcagaaatg cgaaaatgag acggcgcatg gcgcagacat gccgtggatg aagcagcagg
    30361 ccaaggcaaa agatggctca agtgccagcg cgatcttcct tgccgctgcc gtgcttgttc
    30421 cgaactggat cttgaaccgc gccaagccgc caagccaaat gacgaggtcc gggcaggcgc
    30481 ggcgggtctg gttgtttcaa ggttgaccct gcggcgggtt gggcgcatcg ctggttcatg
    30541 cccgtgaaaa ttaccgacga ccaactgccg aggctgctcc atgatcatcc aggctggaca
    30601 gtggagggcg ggcaactcgc gcgcgtgttt gtcttcggga gctatctgga tggcgtggag
    30661 tttgcccgcc gtgtcgcgat cgaagcggag gctttaaatc atcaccccga cattcacatc
    30721 ggctggcgca aggtcactct gcgcgtcagc actcacagcg ccagggccat caccacgctg
    30781 gattttgatt ttgtggagcg cgccggcgcg gtctgggcgg cctttgccgg ctcctagttt
    30841 gccctcctgc atgggaatcg cgggcggcgg tcgcatgaca cctgtcggac gcggggaaca
    30901 agtcgcgtct tataatcggc ggggatggga cgtttgaaaa aaaccaaccc actcccatga
    30961 aacccggcat cctcattccc gccctcctcg cctgcctgtc cttccaactc gccgccgagg
    31021 actggccgcg ctttcgcggg ccgacggcgg atggcaggac gtccgccacg gagctgccgc
    31081 tgaaatggag cgaaagggag aacctgcttt ggaaaacgga gctgcctggt ccggggtcat
    31141 cgagtcccat cgtcagcggc ggcaaggtct tcctcaccag ctacagcggt tacgggctcg
    31201 acagccgcga ggctggggat gccgccaagc tcaagcgtca tgccctctgc ctggatgcgg
    31261 cgacgggcaa aatcctctgg cagcatgaga tcgagtccgc cggtcaggac aagccctaca
    31321 ccgggcaata catcaccatg cacggctacg catccggcag cgcggtcacg gatggctcgg
    31381 gggtgttctt tttcttcgcc catgccggtg tgcatgcttt cactgtggac gggcggaaag
    31441 tgtgggaaaa aagcgtcggc gagaaggcgc acgagtgggg cgccggctcg tcgcccgttt
    31501 tgcatggcga cctgcttttt gtgaacgcgg cgctggaaag cgacacgctt tacgcactgg
    31561 accgccgcac aggaaaagaa gtgtggagcg cgcgcggttt ccccgcctcg tggaacacgc
    31621 cggtcattgt gaagctcaag gacggcagcg aggaactggt cgtgaacgca agcggcaggc
    31681 tgcgcgcttt tgacccggag aagggcgggg aattgtggtc ctgccaggcc attcgcgcgg
    31741 cggagctctg cccgtccgtg gtggcgcatg acggcgtgct ttacatcatc ggcagtccgc
    31801 gcggcaatgc catggccgtg cgcgcgggag ggaaggggga tgtcacccag acgcacgtct
    31861 tgtggcaggc gcagaaaggg tcgaatgtcg gctcgcccgt gtttcacgag gggcatctct
    31921 acttcgtcaa cgacgcgcgc ggcacctttc actgcctgaa ggcggacacc ggcgaggatg
    31981 tgtacgagga gcgcctgcct ggatcgcgcg accgctggta tgcctcgccc attctgaaca
    32041 atggccggct gtattacacc agccgcacca gcggcacggt cgtcatcccg gcggcgccaa
    32101 agttcgaggt gctggccaca aacgtcatcg ccggcgacag cagccccttc aatgccagcc
    32161 ccgccgcggt ggacgggcgg atctacctgc gctcggaccg gtttgcctac tgcatcggac
    32221 ccaggtgagc cgcgtggttt gcgcggcggg gatttggcgg agcggacggg gctcgaaccc
    32281 gcgacctcca acgtgacagg ctggcgttct aaccgactga actaccgctc caaatcccgc
    32341 atgcagggcc gcgatactac aagctcatga tgccctggca aatggaaaaa tcttcgcgag
    32401 gaaaaatgtt ccctgctccc ctcccgaaga ctggcggtcc cgcgcgcggg atgcttgcgt
    32461 ttgcactttc acagccgcgc gctaagtgag ccatcccatg ttagaaaaca ccaaccgact
    32521 cgactcgtgt ttcgcccgcc tgcgcgccgc cagaaagcgc gctttcgtgg cctacatctg
    32581 cgcgggagac cccaccctaa aaaaaacggt ggacatcgtg ctggcactgg agcgggcggg
    32641 agtggatgtg gtggagctgg gcgtgccgtt ttccgatccg ctggccgatg gcgtggtcaa
    32701 tcaaatggcc gccgaccgcg ccctgcgcgc cggcgccacc acggcgggcg tgctggagat
    32761 gatccgcgag gtgcgcgggc actcgcagat ccccgtcgtg ctcttcactt atctcaaccc
    32821 cgtctatgct tacggctacg agcgctttca tcacgatgcc accgccgccg gtgctgatgg
    32881 catcctggtt ctcgatttgc cgccggatga ggctgcggag aatgccgagt tgaagcccgt
    32941 gcccgacctg cgacacatcc agctcatcgc gcccacaagt tcaccggagc gcatcgccag
    33001 catcgccgcc agcgcggagg gctttattta ttatgtctca cgccttggcg ttacgggcgc
    33061 gcaaaccgaa atcgccggcg gcatcgccga gcaggtggcc gagatcaaaa aggtgagcca
    33121 ggtgccggtg tgcgtcggct ttggtgtctc gaatcccggg caagcggccg ccatcgcatc
    33181 catggccgat ggagtcgtcg tcggcagcgc cattgtgaag ctcatcgaaa aaaatggcgc
    33241 ttctccagac tgcaccgcca gtgtcgaagc ctttgtgaag ccgctggtgg atgcggtgaa
    33301 agcgcttccc tgacccagcg ccggacgttt ctcttttccc acatgacact ctccttcacc
    33361 aaaatgaacg gcgcgggcaa tgacttcgtc atgctcgaca atcgcagcct cgccacttcc
    33421 ctgagcaggg agcagatcgc gcggctttgt gacaggcacc gcggcatcgg cgccgacggt
    33481 ctgctctgcg tggagccagc cgaaggcaag ggcgatttca agatgcgcta ctacaatgcc
    33541 gacggcggcg aggccgaaat gtgcggcaat ggcgcgcgct gcttcggccg tttcgtgaac
    33601 cgcctgctgc acgacgagct gagctttgtg caattcgaga cccaggccgg gctgatctcc
    33661 gcggaattcg aggaggaaca agtgcgcatc aacatgagcg cgccgcacag cctgaaaatg
    33721 gcgcaggaac ttctcgtcgc tggcaccacg ctcaccgtcc acagcatcaa caccggcgtg
    33781 ccgcacgcgg tggttttcgt cagcgacctg gaaagcgtgc ccgtgcgcga atggggcgcc
    33841 ggattgcgtt accatgaagc ctttgccctc aagggcacca acgccaattt cgtccaggtg
    33901 ctcggcctcg acagcatcgc catccgcacc tacgagcgtg gcgtggagga tgagaccctg
    33961 gcctgcggca ccggcatggt ggcctgcgcc ctcatccacc acgagctgac cggcgtgccc
    34021 ggccccgtcg cggtcaaagt caaaggcggc gacaccctgc gcgtcggctt cgaggagccc
    34081 gccccgcatg aatactccaa tgtcaccctc ctcggcccgg cggactttgt gttcacgggg
    34141 agcgtggagg tttgatcggc ggcctgcgga attgaaaatt gagaactgga aactgtcatg
    34201 tcatcgctcg aactcgccga acaaatggaa gagcgatttt tggactttgt cgggaggata
    34261 tgcgcggtgg ttgaggcctt gcccgacaca aaaactggca ggcacatttc cgggcaattg
    34321 attcgttgcg gcacgtcacc cgcgccaaat tacgccgaag cctgcgccgc tgaaagccgc
    34381 gcggatttca ctcacaagct gggcatcgtt ttgaaagagc tgcgggaaac gcgcatctgg
    34441 ttgaaattgg tcgtcaaaac cggctccatg aagaaatcca aacttgaagc cctcatcggc
    34501 gagtgctccg agcttatgaa catcatcggc gcctccatcg tgaccaccaa agccaacact
    34561 ccccccaaaa accgccgccg ctaatccagg caccgccttc ctccgccaat tttcagtttc
    34621 caattctcaa ttttcagtca ggaagtcttc cgtttcccca caccgctccc atgttcgcag
    34681 gaacccacac cgccatcatc acccctttcc gcaacggccg cattgacgag gacgcgctca
    34741 aacggctggt ggattttcag ttcgacaacg gtgtcaacgg cgtggtcccc tgcggcacca
    34801 cgggcgagtc gcccacactc gactatgacg agcatgaacg cgtggtgaag ctcaccgtcg
    34861 agttcacacg cggacgcggc atcgtcatgg ccggcaccgg cgcgaacagc acgcgcgagg
    34921 ccgtggagat gacccaggag gccgaggccg ccggcgccac cgcctccctg caagtggctc
    34981 cgtattacaa caagcccacg tccgaaggcc tctaccagca tttcaaaacc atcgccgaca
    35041 acaccagcct gcccatcatg ctttacagca tccccggccg ctgcggcatc gagatcggtc
    35101 tggatgtgct ggagcgcctc gccggggcct gcccaaacat ccgcgccatc aaagaagcgg
    35161 gtggcaatcc cgagcgcgtc agccagatgc gccagatact accggagagc ttcgaaatcc
    35221 tctccggcga cgacggactc accctgccgt tcatgtccgt cggcggcgtc ggcatcgtca
    35281 gtgtcgcctc caacgtcatc ccgcgccaga tcagtgacat ggtgaatctg gctctcaagg
    35341 gtgactacac cggcgcgcgc gccatccatg agcgttacta cccgctcttt gccgccttcc
    35401 tcaagctctc caccaacccc gtgcccgtca aagccgccat gtccctcgcc ggccattgcg
    35461 ccaacgagct gcgcctgccc atgacgccgc tggaggagac caagcttgcc gagttgaaag
    35521 ccaccatgga aaagctcggc ctgctctgaa ggccggccgc attccaaagt ggcacgggtt
    35581 ttgcctccgg ctagcccgtg atgagagtgc ggcaaagtcg gaaatgtcac gcggcatccg
    35641 cctcttccag gatgagatca ggccggtgat tgcgccggcg cagccagttg atggcttggc
    35701 ggacgcgggg gcggctgatg attttgcgct ccagctcctt tttcccgtgg aagtcgagca
    35761 ggaagatgcc gacgaggatg gtcagcagcc cctgaccagg aaggaagatg agcaggatgc
    35821 ccagcgcgac gaagaagacc ccgatcacat ttttgaccag caacacgacg cgccgcggca
    35881 tgcttgccgt tttcagccgc gtccagaggt ggggtttgtc ccgcagaaag tagtccgccg
    35941 gcatccgaat gagcagaagc ggtgtgacaa agacgctggc caggaacatg gccagcgagg
    36001 cgatgctgac gatgatgagg acctggggcg tgagccagcc gctgagcgag cgcacagtcg
    36061 cccaggcggc ggagtcggtg agccagtgaa ggtccatgcg ggttggtggg gggcatttcc
    36121 atcggccagg gccgggccgc gggcaaggag tgtttgcggg ccgtgcgcgc ttccgtcatg
    36181 cgtggaacgc cgtgcttgca aaggccgcgc cggctgccat tctgcgcgct ccccacccat
    36241 ggcaggcacc ccccttctca tctatctcga cggccgtatt gttcccgagt ccgaggccaa
    36301 aatttccgtc ttcgaccacg gcctgctcta tggcgacggc tgcttcgaag gcatccgcat
    36361 ctacaatggc cgcgtcttcc gcctcacgga gcacctcgtg cggctctatg agagcgcgcg
    36421 ctccatctgc ctgaccatcc ccatttccct ggaggaaatg gaaaaggcca ccgtcgagac
    36481 cgtggccgcc aacggcctgc gggacggcta catccgcctc gtcatcaccc gaggtgtcgg
    36541 ctcgctgggg ctgaacccct atcaatgccc gaaggcgtcc atcatcatca tcgccagcgg
    36601 catcacgttg tatccgaagg agaagtatga gaccgggctg cacctcatca cctgcgccac
    36661 gcgccggccc gctaccgccg cgctcagccc gcaggtgaag agcctgaact acctgaacaa
    36721 cgtcatggcc aagatcgagt gcatccaggc cggctgcgag gagggcatca tgctcaacga
    36781 gcagggttat gtcgccgaat gcacgggcga caatgtcttc atcatcaaaa acggccgggt
    36841 cttcaccccc accatcgcca gcggcgcgct caacggcatc acccgcatgg ccgtcatcga
    36901 ggtcatgcgc gagatgggcc tggatgtcag cgaggtcacc atgacccggc acgagattta
    36961 caccgccgac gagtgcttcc tcaccggcac cgccgccgag gtcattcccg ccgtgcaata
    37021 cgaccgccgt ctcatcggct gcggcaagcc cggcaaactc acgctggaga tcatccagcg
    37081 cttcaaaaag ctggccaact ccacaggcac acccgtgccc tacgccgcgt aaccgtccac
    37141 ccagacgaga tgcacgagta ccaccgcctc ctgcgcaaag tcctcgacca cggctccttc
    37201 cgcgaggacc gcaccggcac aggcgcgtgg tccgttttcg gcgagcagag ccgctacgac
    37261 ctcggccggg gcttccccct ggtcaccacc aagaaactgc acctgcgcag catcatccac
    37321 gagctgctct ggtttctcaa gggcgacacc aacgtgcgct atctccagga aaacaaagtc
    37381 accatctggg atgagtgggc cgatgagaac ggcgacctcg gccccgtcta cggtgcccag
    37441 tggcggcgct ggcagggatc tcccggcgcg gagccggtgg atcaaatcgc gcgcctggtg
    37501 gaggggttga aaaacgaccc gcacagccgc cgtcacatcg tcagcgcctg gaacgtgccc
    37561 ttcattgggc agatggccct gcctccctgc cactgcctgt tccagttctt cgtcgcggat
    37621 ggcaggctga gctgccagct ctaccagcgc agcgcggacc ttttcctcgg cgtgcccttc
    37681 aatatcgcca gctacagcct gctcaccctc atggccgccc aggtctgcag actccagccc
    37741 ggcgacttcg tgcatacctt tggcgacctc cacctctacg ccaaccacac cgagcaagcc
    37801 cgtctccagc tcacccgcga gccgcgcccc ctgccagtca tgcgtctgaa ccctgaagtc
    37861 aaagacctct tcgccttccg cttcgaggac ttcaccctcg aaggctacga cccgcatccc
    37921 ccgatcaagg cggagatcgc cgtgtgaggg ccgcgcagga aacgcgcgcc gggtcggagc
    37981 cactccgggc agcgagccgt tccctgcccc cgccaatgcc gcagtctcat gagcctttca
    38041 gttcagtcag ctgccgcgca ccgtcttcac tacccccact ttcgcacata tcggcgaaag
    38101 tgtggctaaa acgcggcggc aagccgaaac ccagttgcaa cgagactccc atacttctca
    38161 catccaatcc gaagccgtct ggcataagaa tgcgcgcgct ccaaaggcgg cggcgcccaa
    38221 ctcctactgc gggccggggc gttgaccacg gttcgaccct caggcctccg ctttcacggc
    38281 ggacacggct tccgatgcgg cggatctgtc cgcccggacg agataaaggc catacaccag
    38341 cagtgccaac tggctgcccc agaagccgta gatcttcggc caggtgccgt cggtgaagcc
    38401 gtaggcgtgc aggccgacgc cgagttggtt gacgccgaac caggagaaga tgacgatgca
    38461 gccgaggatg aggttgcagt tgtgcaggcc gacctcgcgg atgtagccgc cgaggcgggc
    38521 gtgcaggatg acgaggcaca tgaggacgat catgagcgca ccgttttcct tcggatccca
    38581 gccccagaag cggccccagc tgtaattcgc ccagatgccg ccgagcacgg tgccgacgag
    38641 ggagaggaac aatccggcag cgatgaagcc gtaggccacg cgggtcaaaa aacggccttc
    38701 ttcggatttg gggccgattt tgccgaaaag ccgctggatg aagtaaatca tcgaaatcaa
    38761 ggcggcgacc atgcaggcgg catagccgag attgatgagc ggaacgtggg tggaaagcca
    38821 gaagttcgtg atgagcacgg cctggagctg ctgaagggtg tcggtggcct cgctggcctc
    38881 gaactggatg gcgaggaaca tgcaggctgt gccgccgacc gcggcgacca gcaggccgag
    38941 accgcgcttg gtgatccatt cggcggcgag accgaacagc gccaccgagg cgccgatgaa
    39001 gaggatggtc tcatacagcg tggtgatggg gggacgctgc atgatgacgc agcggatgac
    39061 cacgccggtg acggcgcaac ccgtggccgt ggcgagcagg agccatgagg cggccttggt
    39121 gatcttttcc agctttgaac cgggtgcgat ccacgccagg gccacggcca caaaaccgag
    39181 gaggaagatc cactgcgcgt agaaaaagta gtccgctttg atcgaatgcc gctccaaagc
    39241 gatgaaccgc ccctcgtcgc gggccttggc ggcggcttga atcttcgcga gcagcgcttt
    39301 cgcggcggcc ttgaacttct ccgcgtccgg cagggcgagg aacacgtcct cataaaccgc
    39361 cagccaggcg agctgttcgg cggcggcctc cttctcgtcg ttgaagactc cgaagatgat
    39421 ctcgccgggg ccgtgccaga cctcgtccac cttcgggccg ggcgggaaga tgcggagctg
    39481 ctgctcggag ttgccgctca tcatggcgcc gagggccgcc ttgccgaagt cgcggaacca
    39541 cgggatctgc atgggcggtc cgccgctggc ggtgacggcg ctggcgaggg cgttgaggct
    39601 tttcgagagg cgcacgggtt tttcgatgcc tgcgggcagt tgatccacct tgtcaccgac
    39661 gggcgagcgg atgaagtcga agtggccggt gatcatctcg tagtcgagaa agttcgcggc
    39721 aagctgcacg atcatgcgct gctccggggt ctgcttcttc gaggggattt cgcggtattc
    39781 gcccatcttc tccatgagcg tctgccgcgc gggctcgatc tcggcgaagg tgtactggtc
    39841 ccgtttcgcc ctggctgtga ggcccagttc ggtgatggcg gaggagttgt ccaccttgaa
    39901 gagcggcacg agcctggcga tgtccgggcg gaaccagctc atcagcagcc actcggtggc
    39961 gttgagcttc acaggcttgc cgccttcctt gacgatgggc ttctgcgcgg ccggatcggt
    40021 gaccggtttg ccgtccatct cgccattctc ggtgagccag atgctccgcc gcgcgcggaa
    40081 acgcagcagc cggtagttgg cgacattttc cagcggcttg atgcgcccgg attcctgcac
    40141 gggaatggag gcgaaggtct ccacgacttc tttgtcgcgg aaaagatcga gcgggggaag
    40201 atcggctgcc ggcgcgaggg ccgcgaaggc gagcatcgaa agcaaaacaa gacgtttcat
    40261 gactgtgcgg gtgtggcggg agtttcagag cagcttccac attgcgaggg cgaaggcggc
    40321 gttgaaggcg aggatgaggc tgatggagaa cttccacggc ccgaccttga tgaagcgggc
    40381 cagcatcatg aggaagtgca gcagcaggcc ctcggcgacg atgatgcagg agatgagcgg
    40441 ccagtggtcg gaggggttct ccacgatctg gaagccgctg ctcttgacgc cgctgcggct
    40501 gcgcgtgccg tcgtcaaagg tctgctggaa gacggcgtag ccgccgctgc gcacgggttc
    40561 gttcatggtg atgacgcgct tttcctcgcg gccgtcctcc agcttggtca cccagctggt
    40621 gaagcggcgc gcgcgctcgg tgccgggatg cttttcctgc tcggtcttgt cgagcttgat
    40681 ggcgaagggc agtttgtaag tgcggtggac gagggagatc agatatttgt catcaccgaa
    40741 ggtgacggtc cacggcgcgg cggcgaactc ccaaaggatg cccttctgct ccgcgccggt
    40801 ctttttgtcc ttcacgatgg ccgcgcaggc gctggcgagc tgctcgtcgg ccagggcgcc
    40861 gccggagtcg tccagcatgc tcacctcctg gatgaagtag ccgtcaatgg cgtcgccgcg
    40921 ggagccgtcg cggtcgcgcc tgggctgcgc gtgcttcttc caatttgtga tcatcagatc
    40981 gaagggcagg ctctcatggg tgaaggtgcg tcccttgccg gcggagaggt cgcgatactt
    41041 cgactcgtca atgacgagcg cggtgcgctt cgctttttca tccgcctgca ctttctcgat
    41101 ctcaatgacg cggtcatgga agctgagaaa ctcgtcgctc gtctcgccct cgcgcaggct
    41161 catgttcccc tcgacggcaa aatggtagct caccgcgccc gcggcaatca tgaacaggat
    41221 ggcgaagtgc gagatgatca cgccgatggt ctttggcgac ttgcggatgc ggatgatgcc
    41281 gcccagaaac atgttcacga acagcaccgc catcagcgtg tagccgccgg gcaggatggg
    41341 cagcggaaag gtcgcattgc cgatgtccca gtaggcgttc agcgccagcg cgcgccagca
    41401 ggcgccgatg tctatgcgat ccacgaggaa ggactcaaaa tagcgcgcct ggctggccac
    41461 gagtccgtac tccgcctgct ccagcgtgcc gagaaaggtg atgagcagca gcagcgcaag
    41521 cacgatggtg gcaaggccga agctggagag gaaggcgagg agtctcgcga gggggttggg
    41581 ttttggagag gacacggggg aaagctggga aaggcgggga ctgtgttcag cgcccagttg
    41641 gaagacaggc ggaaggagac gagcgggcgc cgggcttgaa aggacggatt tcgttcagcg
    41701 cgatcattgc cggggcacgt tgggcttgat ggactggcag aaggcgtcga acgcggcggt
    41761 gttctgctcg acgagttcct tcgggccggt gagcttcaca aagatggtgg cctgttcggt
    41821 gctgtggatg aggccgagca tgcggtagcc agtcttggcg gtatcggcac cgaagccttt
    41881 gaagtcgcca tcaaaggcca cgaaggtggc ttcgcgattg aagaagggct ttttcggcag
    41941 ggcggccagt tcctcggcgg tgtaggcggg ctggtccatc tgggtgcgcc agcggttcac
    42001 gttcgcatcc aggccgccgg cggagccggg catcaaggag atgtagcatt cgccctcttt
    42061 gtttggacca aaacgcaggt cgaggaggcg catgcccatg gggtcgggtt cgggattctc
    42121 actccagccc tccggcacgg tccaggtgag caggctgcgg aaatccgggc gttgcggctg
    42181 ctcctcccgc gcgtcgtcga agaaccgggt ggcgctggga atgtcaacat tcggcctggg
    42241 ggcgtggatg gaaatctccc gcgactcggt gatggcctcg ctcttgccga gacggtcgca
    42301 cgagggcatc agaagacaag tcgcggccag gaccgcaaaa cgtggagacg gggaaatcat
    42361 gggggacgga acaaacgcgg caaccggcgt ttgtcttcga ttcattacga ctccgggcgg
    42421 atttcgggac gaaccccgcc tgaatgacgc gctccggcgg atgaggcgtc gcgggcccga
    42481 gcgtttgcgc cttgcctagg cgcggcctcg cgccacggtg ggcttcatgc ggcgccgtcg
    42541 gctacatcct ccctggtttc ccggattccg ggcactggca gcattcttcc tgctgctggc
    42601 tctgcttttc ggcttttacc atgcgctggc cgtgttcacc cgcttcgagc gcgcgcccgc
    42661 cccgcccccg ccgcctgcgg cgcggaagta aggcgcggtt cccccttgcc ccggcccgcc
    42721 tccctgccaa tctgcgcggc cttatgcaac acaacgtcta tttctggctc aaaagcggaa
    42781 tgaccgggga ccaagtccgc gtcttcgaga ccgaactcgc cgctctgacc cgcatctcct
    42841 acctggagtc cggcttctgc ggcaaacccg ctcccaccga ggcgcgcccc gtcaccgacc
    42901 acagcttcag ctactccctc agcctgcgct tcaagagcat ggccgaccac gatttctacc
    42961 agaagggctg cccggaccac cagcgctttg tggacacctg caagccgttc tttgaaaagg
    43021 tggtcgtgta tgattcgcag ccgctgggga actgatggcg gtttgtccag gggggcggct
    43081 cagcgccggt ccggctttgc gccctccgcc tcaaagaggc tcggcgcgtt gagcgcctcc
    43141 gcgccgagat aagcgccatc gtgccgccgg tggatgtcgt cctccgtcca catccgccgg
    43201 tccggccccg cgctgcggat gtccagccgc tcgtgcgaaa gggcgtggaa aaacaatggc
    43261 gtgccccagc ggtccaccac ccgcccctgg gcgtcgaagg catggtggtc gtccggcagg
    43321 aagcggatgc gcgcgcggtt ctccccgcgc agcgcggcgg cgatctcctc attggctccg
    43381 agccggaaag gatctccccc tttgacgagc agggccagat ttgccaccgc atgggccatc
    43441 gctgccagat catcctccgc acgactgtca gcgctggcgt aggagcgcag gatgcgctcc
    43501 ccaggcaggg gttccgcagg cggatgcggc atggaagacg cgggggccgc ccctgccagc
    43561 ggcggcgggt ctttctccgg ccaaagcacg ggccgcatca gccagaacgc ggtcattgtc
    43621 aaaaccagcg cccctgcagc cagcgcgcgt ccccgaccgg tcatgcccgc gtccctcatg
    43681 accaggtgcc gggtttgagg aagcccacag gcaggctgga tgagtgcgga tcgtaaaagt
    43741 tggcgatgtt gggcagcaca tagtccatgt cagccgccgc cacgccgaac cagcgcagca
    43801 tttcggcaaa gaaggagtca caggaggtgg aggggatgac acgcccgccg aaacccgtgt
    43861 ccaagctgct gtcgagggcc aggtcgggga agctgccgaa aacccggccc ccgcgcaccg
    43921 ggccgcccat gaccagcgcg ttgccgcccc aggcgtggtc ggtgccgcgc ccgttgctcc
    43981 gcagggtgcg gccaaaatcg gacgcggtga aggtgatcac gctgtccgcc agccccagtt
    44041 gatccagcgc ccgctggaag ccggtcagcg cggcatccat caccgccagc atcccggcct
    44101 gggtctccag caactcccca tggtggtccc agccgccgaa gccgatgaaa atcgtctgcc
    44161 ggtgcagccc cagcgccggg cgcagcgcga tcattttcgc cgccgcgcgg aactgctgcg
    44221 ccacccagtt gccgccaggg aagaggccgg ccaccggcga gtcatcgtag ctgttgaact
    44281 gctgcaaaaa gaactcctgc agctcgatgc tgcccttggt cagcttcgcg taggcctcct
    44341 gcatcaggct cgcgtagctc tgctcgatga tcgagcgatg cgccgcgttc ttttggaaaa
    44401 gcggatgcgc cggcgaggtc tcgccgctgc cggtgaatgt cagcgccccg ctggaggtca
    44461 ccacaaactg agcggactgg ttgcccacct ggaacaggtt gttcccggag agcgagatgc
    44521 ccatcgagag cgcgctcgtg ttcaccgcgt gcagcacatc cgccgcgcgc ccgccccatc
    44581 cgctgagctg cgtcatgccc tgaggcaccg aggtctgcca ctggtcaatc tggtcgctgt
    44641 gcgaatatag cgcgcgcggc agcggcacgc ttgccgccag gtattgggcc ttggtcaccg
    44701 gctggatcag cgtgcccaca ttcgccagaa aagccgcccg cctttgtccg ccgctgacgc
    44761 cggtgccgtt gaaaaagtcc gccaggccct ggcaggaagg atgcagcccg tagagctggc
    44821 cgtcaccgcc cgcgtcctgg tccaaaacct cgagatcgcc ggtgccgaga gccaggtcac
    44881 tgcgtgtcgt ggcatacacc gcgtgccgcg cggcatccct gggcaccagc cagttgaagg
    44941 aatcgttgcc gccattgaga aaaaggcaca ccagcgtccg gtcgtccgcc ggcgcgccgc
    45001 tggccgccgc gtggttcgcc agccgcaggt tcagcagcgt gttgagcacc gaaacgccgc
    45061 tcaaagcgga gcaggaggct tggccgagaa agcggcggcg gttcatggcg gcgggcatgg
    45121 cggcgaaaac ctgcccgcat ttcaaatccc ccgcaattgc aaacaccggc tccagcgccg
    45181 catgccgcgc catcttcatc ctccgcttgg cattgaatct gttctcttga tttgccccct
    45241 gatttgaaga aagaacgccc agcctcccga atgccgtccg ccaacccagc agcacgcacc
    45301 gccacgccgc cgcatgaagc gcccgctggc ggcggcctgc tggaggggta ccggccgcag
    45361 cccggagtcc acgatgagat gctcctgccc ggcggcgccc tgcgcccgca ttggcgcacc
    45421 ttcggcagct tcctggccca gtgcggaccg gaggacatcc aggcgcgcgc cgccgccatc
    45481 cagcgtctgc tgcgcgacca tggtgtcact tacaatgtct atgacgacgc actcggcacc
    45541 agccgcccct ggtccctcga tgtcctcccc ttcctcctcg gcgaggacga atggctccat
    45601 gtcagcgccg gcctcgacca gcggtcgcgt ctgctcaacg ccatcctggc cgacctctac
    45661 gggccgcaga cactcatcca ggctggcctt ctgccgcccc gcatcctcca cgccaaccct
    45721 ggcttcctcc gccccgcagc cggcatcacc ccgcccggcg gccgctggct ttcccatctg
    45781 gcctgcgacc tcgtgcgcgg cgcggacggc cgctggctcg tcctcaccga ccgcgccgag
    45841 cgccccgccg gccacggcta caccctggag aaccgcgtca tcctctccaa cgtcttcccc
    45901 gaagagttca acgcctcccg cgtccgccgc ctcgccgccc atttcgactc gcagcgtgaa
    45961 atgctccagt ccctggcgcc caagacccgc catggccgcg ccggcatcct catgctcacc
    46021 cccggcccgc gccacgagac ttattacgag cacgccttcc tcgcccgcca cctcggcttc
    46081 cccctggtcg agggcgccga cctcaccacg cgcgaccgcg ccctatatct caaaaccctt
    46141 gaagggctcc accgcgtgga tgtcatggtc cgccatgtgg acgaccgctg gtgtgatccc
    46201 ctggaactgc gcgccgagtc tctcatcggc gtcgccggcc ttgtcgaagc ctggcgcaac
    46261 ggcagtgtgg ccctggccaa cggcctcggc gcgggcatcg tggaaacccc ggccctgcac
    46321 cctttccttc ccggcatatg ccgtcacctt ctcggccagg agttgctgct tccctgtgtc
    46381 cccacctggt ggtgcggcca gcagagggag cttgaactgc tcctggccga accctcccgc
    46441 tgggtcgtca agcccgcctt cgcacgcggc gcgcgcgacc ccgtcttcct cgctgatctc
    46501 gactccgagc agcgcgagcg ccagctcgaa tccatccgcg ccgcccctca cgaatgggtg
    46561 gcccaggagg ttctgcccct ctccaccacg cccgcgtgga ccggctcgcg catcgaagcc
    46621 aggcccctgg tctggcgcgc cttcgccatc gcccgcgggg aaggattcga gaccatgccc
    46681 ggcggcctcg cccgtgtcag tcccgagccc ggccgctggc tcatcacgct gcgctccggc
    46741 ggcatcagca aggacacctg ggtcatctcc agcggcgcgc acccccacgc cccgcaggct
    46801 cccgcccgcg aggccgccgc c
//
LOCUS       DAOMYJ010000013        45703 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Prosthecobacter sp. isolate AMR_MDS_5629
            NODE_318_length_45703_cov_22.519979, whole genome shotgun sequence.
ACCESSION   DAOMYJ010000013 DAOMYJ010000000
VERSION     DAOMYJ010000013.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867850
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prosthecobacter sp. (wastewater metagenome)
  ORGANISM  Prosthecobacter sp.
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Verrucomicrobiaceae; Prosthecobacter.
REFERENCE   1  (bases 1 to 45703)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 45703)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.30
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:34:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,742
            CDSs (total)                      :: 4,684
            Genes (coding)                    :: 4,676
            CDSs (with protein)               :: 4,676
            Genes (RNA)                       :: 58
            tRNAs                             :: 55
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..45703
                     /organism="Prosthecobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_318_length_45703_cov_22.519979"
                     /isolate="AMR_MDS_5629"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1965333"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..936)
                     /locus_tag="PLP58_02435"
     CDS             complement(<1..936)
                     /locus_tag="PLP58_02435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ07411.1"
                     /translation="MSFISFSLRPAASAALSVLLFQAAAAHARGQDQAGIEKALEGLP
                     PEVRQSVRLQPDGMDLPSASADDPLSLAVRAASLVSGDTETQNRLLHDCGRQAVRQGR
                     TATARELAARLTDYRAALLLLEIAEKEAGKDRGQARGLVMLAAGMTGMIKPWQAELVL
                     ARLVHAGALLELEGDVTRVWWESIRDPETRFASGAALMTLESAKTGVFDLARLRSGQD
                     AKTRGKPVPALLEVSRRLFGQALERLASTDARQQALGTALVESGIEVLAISNVPKAEV
                     LVETAVGFFQAGHEDLARRIFSVVEDRLAAPHEEQA"
     gene            complement(1004..2938)
                     /locus_tag="PLP58_02440"
     CDS             complement(1004..2938)
                     /locus_tag="PLP58_02440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ07412.1"
                     /translation="MKHTIKATFLAAAFAAGTLNEARADFIVVGSATDNRITTDTRWT
                     RDNVYILGRVIIVSNGAKLTIEPGTIIRGLRALESDIAEEPGSLVIARGSKLVANGTV
                     DAPIIFTSIDDPNVPGGTATVPASVTNLLGRVIDVTALANNDYSPGGPSGNNGFAKSG
                     QWGGIILCGRGHVAANTGNTDADANGIWDAHGDSLTENFRRIQNAGVGTDYPEGLSSG
                     SGSNVLNSELTIYGGTDDDDDSGVMRYVVNRYGGFVIGAAAVGNEINGITICGVGTGF
                     TFEHCEVYQNKDDGFEWFGGKHDTRHLFSSANQDDAFDGDEGFRGDHQFWTVIQGTIN
                     TSGGALRSGHTGSNGTIGQIESGSDYQYDKLMEWDGGEPDNGDRLPMTDISLYNFTML
                     TGGTKFEGLNPKLEAHVAMHNGVIENVNAVSRAAETGGGAITTLLTWSNIFAYRATAT
                     AGNPLPASSTVSVGATTATVGGVVIPILSQVNALTFPGVSQIRTPWRFSAVSNPVVPC
                     PLYTKNGLDPRLDPAAAAYGNVPFAVGLPGGLVNAAFAGSMRDNNQMFGWTSLHALEA
                     FVDDNLVRPEISLGVSGGNPTVSFQSVGGVRYVVEKSADGKRWAVLTTEPLVGTDGIV
                     VHTDTTSDEDDTVLYRVYGL"
     gene            complement(2988..5804)
                     /locus_tag="PLP58_02445"
     CDS             complement(2988..5804)
                     /locus_tag="PLP58_02445"
                     /inference="COORDINATES: protein motif:HMM:NF025264.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="immunoglobulin domain-containing protein"
                     /protein_id="HRJ07413.1"
                     /translation="MKKILLTTALMLGLQGAVQAAPARLFASNLGNGIAPSNGLVGIS
                     TATVRFGYFPDAYDFSGKTFAQLNADFTQVAQSTAPLFVDGQRGFFDLDFDYETGSMD
                     GKKIHVWILNGATAATAPQQAIFSTSQTFVTPDGIFPNHSFISPDTGVMDLVAHVGDL
                     ADGADIGGAANAHTTVGDDYRVTDATASRTPEDEVIFQGTAVTFTAIADSSFNGIKYQ
                     WRLNGKNISKANSETLVIPSVTTKNTGNYDCVVTDDTVVVTTNVVPLQVLGIKPTFTM
                     QPMSDVVAIGSAITLNAQAVSPNGVNYQWKNKASLPGATDPQIEIANARPDDAGPYIC
                     VATNAAAIQGGGSTNSLVAEVVVIDVQTREIGGPVGGTVKIDAIYFGKAARFQWKRNG
                     VDLTNGGQFSGVTTKSLGIKGLTNGNDGDVYTCQIFAGNAVAGFDSLESGDFNLSVFN
                     GAPTFTTVDNPIVLPPATLGQDYTPFQIPANGASLFAVSGLPKGMVCHPQTGFVSGRP
                     MALNKDPLVPFNLTITITNGKDKLARPATLLVNNILANLNGAYTAWVARSTFSGGYSH
                     TNNLGGRIDMLITTLGSLSGNLFLGDAKPIKFTGFLNNPGAASPTATILIRRPGLSFV
                     PLEFVFTIQTPDNGDTARKVLSSAVLRDAFLVPGQTGIRSTTVTGWRRKFDSKNPTAT
                     FPGIYNVAVTASGDSATRPKGATLATLTIKPDGSVAIVGTTGEGEKITGSTFVGPTGQ
                     VLLFNLLYTPLRGSIVGNLQLNPGDVVVKDTVGGTLTWRRPANTSTKTRAYAGGFNLA
                     SGDLTIAGGRYAITDKNRRILDLAADTAALLEFSDAGLSPANTPARPDINVTLQAGNK
                     VQINTANPPRLTKLVVNAAKGIFSGTFTYDGVANKTVKFQGHVIRNGGEMGSWGGFGW
                     FMLTQVPGTTTSPVQSGLVTLGIP"
     gene            complement(5837..6841)
                     /locus_tag="PLP58_02450"
     CDS             complement(5837..6841)
                     /locus_tag="PLP58_02450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ07414.1"
                     /translation="MIRPSHTTLALVTLLALGATQASGQADKLPMSRTAGTVTVALPG
                     GATSLVGISNVKIVASGTVGAVAGGDLTLVSSPAALPDVLAAPHALKIVSRANPAGNN
                     AYGKSATITAQAGQVVTAALSTGPNVGDEFVIYQLATLGGLLGAANETGLTGAAAPGA
                     ADKVYLTDAGVLVAYFFNTTAGKWRLASDPGGADQENTVVKPGSGLMITRVAGPDRTL
                     RLQGDTLPARHAADVAAGHNIVNNPFLIATSLSASDIQSNITGGTGPGVADIVYLEEG
                     GVLTGYYFKTGGPGGTGWRALGDSVTEAGGALIRPAKALLFKEQAGTAGFALTEPFAD
                     "
     gene            complement(7052..8023)
                     /gene="bamD"
                     /locus_tag="PLP58_02455"
     CDS             complement(7052..8023)
                     /gene="bamD"
                     /locus_tag="PLP58_02455"
                     /inference="COORDINATES: protein motif:HMM:NF024915.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamD"
                     /protein_id="HRJ07415.1"
                     /translation="MKAGLLCLLAAMGIHAQDQDARADETAALLLQRAQKALAAGDDR
                     GALERCAVIQKKYPRTEWSAQALWHTAAIHQKHAEHNAAFEALDQLVNRQPGHFTRAH
                     EEQLRLALRLLGLARGQRRSLEPVKKADMAPQEDIIAMLARVILNGPHSETGVQAHYW
                     LGVALEKAGRIEEARAAYGDFVEKHPAHELADDAGYQAAEIAFKHWKEMRGAAPKDRE
                     RAAVLMTWFLARFPESEKTAQARASMAELRVAEERELSSLAAYYEARGDAKAAAIYYR
                     ELALKFPGLAMEGSPLRDKLASVITSKTPPNDARQTVGPEASEAAGK"
     gene            complement(8061..8549)
                     /locus_tag="PLP58_02460"
     CDS             complement(8061..8549)
                     /locus_tag="PLP58_02460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ07416.1"
                     /translation="MIPRFTSLILAAAILAGLSSCKTMTSDPVVIGDSPVTYRLVSEI
                     VDEETLEHSVKFRNIGQQVVSFDYTLADDASVPHLDCLGPNSGLVENLYPGAEATVKN
                     TLGKSRGVHVVLGRITAGKKSGEQLARVYKPSTLPPPMPASGSGGLLPLPTLDSASTS
                     SE"
     gene            complement(8585..9358)
                     /locus_tag="PLP58_02465"
     CDS             complement(8585..9358)
                     /locus_tag="PLP58_02465"
                     /inference="COORDINATES: protein motif:HMM:NF024895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HRJ07417.1"
                     /translation="MRLRVILPPAFLILAIAAACLHPSGAAELELKSLGAVGEAKEKR
                     KFGPFVSVMAGETLGQTGTAKVGERNFPVEDIDGSAIFSVEVGKTWNMRRVPVEFSLS
                     AEATFTSTTLQGSVPAANLNNDQIAEYQSDMNSLIFSLNGTFALDLYRYRARIGKVWG
                     GFKPYVGGGLGGGQVWFRNSTAKSADQVQGGTTEPGEAVPFSIDEFINVWNWRAGLEW
                     SWEERYSVFAEYRKTYMGDLDDLRGFRNDGYLVGFRYRY"
     gene            9477..11198
                     /locus_tag="PLP58_02470"
     CDS             9477..11198
                     /locus_tag="PLP58_02470"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HRJ07418.1"
                     /translation="MPHKLTDRRITASPALPALGVLLAGVMMAAGGEKDKAAQPDAVL
                     FPLAPRAQPAQPPLPREGFPMDSGNYQNPLIIFTEGPASPPLPDEMNPDLLPPLLPML
                     EEDAAPAPPPTAAQLRLMALGEKVAAAVVGIRVWDSLGVQLASGIGSYVTTDGILLTD
                     AGLLHPEIAEKTGYITLVRADGTSTRAAGFYLVDQASGVALLQSEDFDTTPIELAPTA
                     DFSRERAAHVLAVSEKRGLVLAEARVQADPAITALGWLNVRGDDSPGAAGSPVLDEEG
                     QAIAIIGMVVPLKEWKNFALPVDAAALALRQKRAPLRPLSELPRTPGLRQVVQDPAFV
                     EAFQTLEQKRLEPAMRKLVQLTRKYPRSAECWALLGLAASRLGAGPEAVNCQRKAVAL
                     DPKAGLYWHQLAFAKMRESPSPGILTGEDCEALSRAVEQRPDDALAWLLLASHFARAG
                     DLDQADAALRRVTVLSPAYAQAHYLQAYVRGRKGDYAGAETSIQRALQLDPKHAEAWY
                     YQGLLHDKKGAPASAAKAWRNTVRLRPDHPQAWLNLAHAHKKAGQATEARQAILEHQK
                     VRSAAAH"
     gene            complement(11243..11830)
                     /locus_tag="PLP58_02475"
     CDS             complement(11243..11830)
                     /locus_tag="PLP58_02475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009958205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ07419.1"
                     /translation="MIGVYDFCGHYEWTFDWLEKLGGHDLVRAYWDEAIRQDSQRHAE
                     ALIMAGGFDGMEEYWGHTLGEEGGEWEVTKTEAVFRIDMHHCPSKGFLLRNGLEQYPD
                     YCDHCMGWIGPLMREAGFVVDHEHNHCGQCWWEMRRATDESLSSAPGTVSGAADARLQ
                     AGWKTADTRMDCYTRANDPDDKRRVSGPHGSPAGE"
     gene            11933..12367
                     /gene="rnpB"
                     /locus_tag="PLP58_02480"
     ncRNA           11933..12367
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="PLP58_02480"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
     gene            complement(12402..13370)
                     /locus_tag="PLP58_02485"
     CDS             complement(12402..13370)
                     /locus_tag="PLP58_02485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009964619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HRJ07420.1"
                     /translation="MSLLTVKNLQQHFPVRGGVLRRAVAACKAVDGVSFTLGEGETLG
                     LVGESGCGKTTLGKAIVRLLKPTEGKIVFEGQDITHASMRAIRPQRRHMQMIFQDPAE
                     SLNPRHTVRDILEEPFIVQKIGSKEERRKWVFELLDKVGLSSASADRFPFEFSGGQRQ
                     RIGIARAIALKPKLIVCDEPVSALDVSVQSQVLNLLLDLQREMGLSYLFIAHGLSVVK
                     HMSDRVAVMYLGKIVELAPSEELYRNPRHAYTKALMDAIPVPDPAKRRKRQLLQGDVP
                     SPINPPAGCAFGHRMKHPKWEESTRMDLALKEVAPGHWVQPCPCCT"
     gene            complement(13367..14350)
                     /locus_tag="PLP58_02490"
     CDS             complement(13367..14350)
                     /locus_tag="PLP58_02490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009964620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRJ07421.1"
                     /translation="MSEPLLQVENLVTAFQTDSGLMTAVDGVSWSVTAGKTLGIVGES
                     GCGKSVTAFSINRLLPQPHGRILSGRILFEGRDLVTMPDEELRKLRGSEIGMIFQEPM
                     TALNPVHKIGRQLTEALLLHQPELSPATAQQRVIEMLQRVRIPSPENRIHEYPHQLSG
                     GMRQRVMIAMALINKPKLLIADEPTTALDVTVQAQILELISDLQKEMGMSVILITHDL
                     GVIAEVCDEVAVMYAGRIVEKAGVFDLFANPRHAYTQGLLESIPRLDSTPKTLLKAIP
                     GNVPGIADFKPGCRFAERSGREHTSEHLTTRPPVTEIAPGHFVEHCPICVA"
     gene            complement(14360..16285)
                     /locus_tag="PLP58_02495"
     CDS             complement(14360..16285)
                     /locus_tag="PLP58_02495"
                     /inference="COORDINATES: protein motif:HMM:NF012706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HRJ07422.1"
                     /translation="MKTMLKLALLLAAAFLASCGGKKEAGSTTDITPEVLKFYEETKN
                     EKGEPFFVTKTATDLPADLKWEDGSDQEEFGSPDAKKGGTYRFFLTDYPRTLRHIGPD
                     ANNEIRDYVQDSVLIKMLGRHPNTLEMIPGCAKEWARGADKKTFYFKLDPDAKFSDGE
                     PVRADNFFFSFFYYRYPHNQATWYHDFFSKKYEQIIKYDDLTLAVVVASAKPDAIEYA
                     GALQPTAIAFHKEMSPDYLQTYNWRFIPTTGAYEVREGGLKKGQSVTFTRIQDWWAKD
                     KKFYRQQYNPDRVVLEVIRDPDKAIEVFKKGDLDAYRLNLPERWHKSVPDDDPMVAKG
                     YLHKATFYNQVPRPTYGFWLNTAKPLLGNQDVREGLQHSCNWDVVIQQIFYGDYERLN
                     LAEEGFGEFTDTSIKARTFDPVKAAESFAKAGFTQRGKDGIFADAKGQRLSFTITNSY
                     KVLESALVVIKEEARKAGVEFNVETPENTAGWKKFNEKQHDIAFTAFNVSVEMYPRFW
                     EMWHSVNAYTQEGKLAVDNNNFTSFNDKEMDAMIDTYDKSEDIAEMKELAWKMQRRIH
                     DAAVFIPGFKSPWYRTGHWRWMKFPAHFDARTARDHEENWLFWIDEDERKATQEAMKS
                     GKTFEKSVKVYDQWRQK"
     gene            complement(16285..17349)
                     /locus_tag="PLP58_02500"
     CDS             complement(16285..17349)
                     /locus_tag="PLP58_02500"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HRJ07423.1"
                     /translation="MFRLNPLTAKKWQRFRALKRGWWSFVILLILCLLALVAELWINK
                     RALFVSHEGQWHFPTYAGFKSGRTFGLDYDYEANYRELQAKWKAEKSESWLIMPIIPW
                     DPYENDFRDDVQHPQPPDAARRHWLGTDKIGRDIAARLFYGFRVNMTFALCYAAGVYL
                     LGIIAGCTMGYTGGRTDLYGQRLTEIWSLIPFLYMVMIAISLLPADMKVVQRIVILLV
                     IMIVFSWTGLATYLRTVTMKEKARDYVAAASLLGASNMRLIFRHVLPNTLSTLVTFLP
                     FTIMSSISALNALDYLAFGLPPPTPSWGDMLHEGVSQLSSPWIVLSAFSAITFVLLLV
                     AFIGEAVRDAFDPKKFTTYR"
     gene            complement(17362..18381)
                     /locus_tag="PLP58_02505"
     CDS             complement(17362..18381)
                     /locus_tag="PLP58_02505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012420883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HRJ07424.1"
                     /translation="MRDYFIRRFLLIIPTMIGLTLLVFAITRFVPGGPMERAMMQFRM
                     ASSEGRSSGPGGGGASLSESQMQQLKAFYGFDKPWPLAYADWLGKVCQGDLGTSTRYT
                     EPVWDLIAERLPVSTYFGLMTFLLSYGICIPLGVLKALKHQQPVDTWSSVFIFAGYAI
                     PSFALGALLVVFCAARWQWFPVGGFTSDEFDDLSLLGQAWDILQHTALPLICYLVGSF
                     AFLTMMKKNSLLDNLAADYIRTAVAKGCDFKQAVRRHALRNSFIPIATSLGHNLTLFI
                     AGSFLIESIFDINGFGLLGYQSLLDRDYPVVMGQLVIVGVLTLLGNILSDFFVALTDP
                     RVRFE"
     gene            complement(18408..18899)
                     /locus_tag="PLP58_02510"
     CDS             complement(18408..18899)
                     /locus_tag="PLP58_02510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009957971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SufE family protein"
                     /protein_id="HRJ07425.1"
                     /translation="MSYPAALQEIIEFFELLPEPERRENLIHYSDAAASCVRKEGQVF
                     DLEDVRKDEECTDTVGVFLHVDEAGAARFAIELGPKVQTLTKAMTAILCRGLNGSTPE
                     EVLAVPADFVPRIVGADLVRLRSQTVYYVLSRMKTAVKMLLDRRRAQAADASGRNTSL
                     PAA"
     gene            complement(18912..19778)
                     /locus_tag="PLP58_02515"
     CDS             complement(18912..19778)
                     /locus_tag="PLP58_02515"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009957973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase"
                     /protein_id="HRJ07426.1"
                     /translation="MNPKFAEYSQPDVLVDTEWLAANLDTPGIRIVESNEDLLLYDVG
                     HIPGAVHIDWRRDLNDQTTRDYVGPEAFAELCRRNGITPDTTVIFYGDKSNWWACYAF
                     WVFQLFGHTKAKVLDGGRDKWEAEGRPLTRVVPKPEPAEYPVPARRDDASHRAYFQQT
                     LEHMRAGGRLVDVRSPKEYSGEMTHMPEYPQEGVLRGGHIPGARSCPWARAANEDKTF
                     KSRAGLEAIYAGELGLKTDDDIIAYCRIGERSSHTWFVLKYLLGYQKVRNYDGSWTEW
                     GNMVRTPIEKSI"
     gene            19871..20545
                     /locus_tag="PLP58_02520"
     CDS             19871..20545
                     /locus_tag="PLP58_02520"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF016277.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HRJ07427.1"
                     /translation="MIHQAFECCSVPPETMDRLWAAGWRHFGPRFFRYSLDMMDDRPV
                     TITPLRLDLAKFTPGKGQRRVLRRNADTRCEFVPASLSTEARAMFHAHKSRFKTNIPD
                     DLGDFFSPEPAIVPCPCLECRVWIGDELAAISFLDIGATATSAVYGMFDPARSRRSLG
                     MLTMLREIEHSRALGRRFYYPGYATLEPGPYDYKKQFAGLSRLVWESGEWLPLPGDLS
                     MPSNAA"
     gene            20630..21358
                     /locus_tag="PLP58_02525"
     CDS             20630..21358
                     /locus_tag="PLP58_02525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ07428.1"
                     /translation="MQPRLSLILPLACLLLAGCAASGPSDPAGADIEESGFKLTKTRG
                     RNTGTNLHKLSRARRQNSPPREKPSRSRVPGMEAPVGDPSVIETPGAGPLQADARNAG
                     PLPDAAPGAQPPAPPPFSKDPGPHTSIVPAARAGGWSPVGDFAGVRVWWRFTHGPEGE
                     VECDFKMENSNPYPVVIFFDPTFTAASGGKDRGKSVRVTLRDGQSRTTPGGRFEAAEG
                     KTEGLLTRPPAAGGVSDMTVAPVL"
     gene            complement(21413..22660)
                     /locus_tag="PLP58_02530"
     CDS             complement(21413..22660)
                     /locus_tag="PLP58_02530"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HRJ07429.1"
                     /translation="MPYLVVLYVIAYLDRVNVSYAALEMTADLGFSAETYGLGAGIFF
                     VGYFLLEVPGAVFAQRWGVRRLVCRIMVSWGVLAMLMGFIQNAAQFYWLRFLIGAAEA
                     GFFPAMIVYLGQWFRAVDRGKAVAWFMSAISLAMVIGGPVSGALLKLDWLGLEGWRWL
                     FILEGLPAVVLGVSVLFFLTEKPQDAKWLPEDEKAWLVAELDRERLAREAMQARKPTV
                     WQALHDPRVLLFSAAYFFGLIASNGLGYWLPTMIQSLSGFSNLTVALLVSLPYSLGVI
                     AKIAAGWSSDRMRERRWHTAGLLLLGGAGLGSLVLWQGHLGIALACLCIAVTGLTGYT
                     PSFWAYATSFLAGTANAAAIGLINSIGNLGSFAGPYAMGWLKERTTGYTAGVLVLAAS
                     CVVTALLVLVARRFERQDGKSPS"
     gene            complement(22736..24361)
                     /locus_tag="PLP58_02535"
     CDS             complement(22736..24361)
                     /locus_tag="PLP58_02535"
                     /inference="COORDINATES: protein motif:HMM:NF012471.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004181 - metallocarboxypeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M14 family zinc carboxypeptidase"
                     /protein_id="HRJ07430.1"
                     /translation="MKLIALVLLSLGALLCLAQTNDVPKDRLDELPQPWKEPIHRISF
                     EEYDATLKYWAEQHPGIFTFEKRGESHDGQPVYLVKITDSTVSSEDKQVVLVSALHGG
                     PERSGTTTTLHLIEWLLGDSDLAKETRRKQVVLAVPIANPYAFFTTDRFTNKQKIDLY
                     DPQISYWDLPKLKLAVPQRTPELAAFLGIVDEYQPEMHLDLHGTGLQAFPDDKLGDRT
                     MRQGQTMFEISACSYSNCGVRPWDPRVTEAMVQAGAAAGFGSDRAEADAQRCFYNPDH
                     DVFRSRLWNQPRPDRFRTMFYGYMKYHTMISTTEIGWEQSGVARVCGILALGNKQWVD
                     EKFSGYPVTRVRYRAGRFVTAYGQTATQRRQSRVELWNRQPAFADGMMYPEYEGRATY
                     VCAVTPKGMAALDNAPAKFIANLRQLPGVNADAIERFVKLGPEYEFTSDPREATQGDP
                     VQNGIGFRFRVPYRSPTLLNVAVNGHTLTESATDGYQAWYADGYTQVQINVPPEKTRT
                     MDLYVVTCAYNGNEKRSYGWEPPAAVKEKLKAK"
     gene            complement(24358..25398)
                     /locus_tag="PLP58_02540"
     CDS             complement(24358..25398)
                     /locus_tag="PLP58_02540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007413175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virulence RhuM family protein"
                     /protein_id="HRJ07431.1"
                     /translation="MSALIPAPPPGGGEFLFYQSDDGRIRLQVRLEDESVWLSLNQMA
                     DLFQRDKSVISRHISNVFEEGELMRNRVVANFATTAADGKTYQVEYFNLDVIISVGYR
                     VKSHRGTQFRIWATQRLKEYIVKGFAMDDERLKQRGGGNYFEELLARIRDIRSSEKEF
                     WRKVLEIYATSIDYDPGTEASRQFFATVQNKMHWAAHGQTAAEVIHARVDGTKPQAGM
                     TNWLGANPGKSEAVIAKNYLTPEELNALNRIVTAFLELAEVQALNRQPMYMSDWLARM
                     DDFLRLGGRDILTHSGRISHEQAVKKAELEYDKYHRAELAKPSKAELDFDTAVQELKK
                     LPATPARKKKKP"
     gene            complement(25400..29305)
                     /locus_tag="PLP58_02545"
     CDS             complement(25400..29305)
                     /locus_tag="PLP58_02545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ07432.1"
                     /translation="MPAALNTTRARQRLQAFEFRPLFIEELGWEPPASRKVASFTPPK
                     SSAAFDITEIAQLAGFRVFEAIAQDGQLPDAAAQLAVSKHVSAASHEHILIFADAART
                     RSLWFWVRREQGGKKRIPRRHHYLRGQPGDLFLSKLAALFVDISELDENGQFSLTEAA
                     TRVQRALDVEAVTKQFFRDFQTEHLKLLDLIKGIPDPRERRWYASVLMNRLMFVWFLQ
                     AKGFLAGENRDYLPGLLTASQQAGKNRFFSHTLRDLFFEGFAKPSAKRQPVGTVPLAD
                     IPYLNGGLFLPHGIEQRIEGDSLLTAAYAKIKIPDEAFTEIFAIFRRYSWSLNDTPGG
                     DDREINPDVLGYIFEKYINQKEFGAYYTRPEITEYLCEQTIHQLLLDRANAGHDTAAK
                     LHLKNPKSFPAPRRYTGIDDLLFHADGPLCARLLHDVLPRLSLLDPACGSGAFLVAAQ
                     KTLLNLYTALLGRCEALNHKPVLDWIAREKQAHKAPAAYWLKKKIVTENLYGVDIMEE
                     ATEIAKLRLFLSLVASAEKRDQLEPLPNIEFNILAGNSLIGLLHVDASAFDGSNHQTG
                     TSPNRTSPADSRRPADSFRGKSAGLPESAGTISGVSGSAQSSIVNHQSPGQQRFTLTH
                     APAADELGLTIESTTAPTRSEREAAHLATVRAAKYAELLAEKNRLIALYKKAPELIGT
                     AAAKDSDSLVTLRRSIEQQKAAAREVLDKLLLDEFQRLGIQFHQATWDSAKKKDGRPE
                     KRTLRLEDIRALQPFHWAYEFDDILITRGGFDAIITNPPWENFKPDDKEFFMPLAEDV
                     SKNKMRLEDFIERKTDVLTGDSALREKYLAYLSRFPHVNLWFRNSEQYENQTAIVNGR
                     KATTDLNLYKLFLEQTLHLLHTGGYCGIVIPGGIYSDLGATRLRQMLFEKTRITGLFG
                     FENRKQIFENVDSRFKFVVLTFRPGDTTTKFPAAFMRHEVQELSTFPNEDSINTEIEL
                     VRKLSPDSLSLTEFRAPNEVALARKLLSFPLLGETIEGQWQLFLHREFHITDDAALYR
                     PQPAKGLRPLVEGKSFHQFVYPFSEPRYWVDMSEARTALLPGRLRFIQSLAKEHKIKA
                     TVSPDAVKLDYQSYRLAFRDIAASTNERTMIASILPPNVVCPDTVRLDEVYFDSVQDG
                     RLMLNQTHFSNHVRLCVVALFNSFIVDYLIRQKVTSHVSLFYVYQLPIPRLTAQDAAF
                     RPLVERAARLVGTSAAFDDLLKEVFGPKATHRSHGVPDPATRQRLRAEMDALVARLYG
                     LTEPEFTHILATFPLVNESVKTETLNTFRDLLKTGQLPS"
     gene            complement(29358..29915)
                     /locus_tag="PLP58_02550"
     CDS             complement(29358..29915)
                     /locus_tag="PLP58_02550"
                     /inference="COORDINATES: protein motif:HMM:NF022018.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ORF6N domain-containing protein"
                     /protein_id="HRJ07433.1"
                     /translation="MTSADHAIALQAERRILLIRAQKVMLDFDLAELYDVETRALKQA
                     VRRNPERFPEDFLFELTDEEVEEMVSQNVIPGRGKLGGATPYAFTEQGVAMLSSVLRS
                     DRAVQVNIAIVRAFVRLRQMLSTNAELARKLEAMEKKYDGQFKVVFDALRQLMAPPPA
                     GSKKEIGFHTFAHSKGGKPKSRAKR"
     gene            complement(29912..30118)
                     /locus_tag="PLP58_02555"
     CDS             complement(29912..30118)
                     /locus_tag="PLP58_02555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ07434.1"
                     /translation="MNTLAHAQEQARRFNEAEGVQHEFRHLRMLRRLTSPQLVLPEDV
                     ADDLDWQQALTTCRQTRAISLHLP"
     gene            complement(30133..30492)
                     /locus_tag="PLP58_02560"
     CDS             complement(30133..30492)
                     /locus_tag="PLP58_02560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ07435.1"
                     /translation="MKKKTAPSAPRSNFDGIIRLVEGLQTLQAQVADSLAPEVDGIIR
                     SKCRDAARIEHALDTLLDACGHPRVLALFRRLCRHYFYISPVVTAEYIHLYRDTWEPE
                     SLPPAPAQRTPKPRAKR"
     gene            complement(30511..33981)
                     /locus_tag="PLP58_02565"
     CDS             complement(30511..33981)
                     /locus_tag="PLP58_02565"
                     /inference="COORDINATES: protein motif:HMM:NF024491.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase D-like domain-containing protein"
                     /protein_id="HRJ07436.1"
                     /translation="MPRIFDNIAQDLLPALQQTLALGHRSDFCVGYFNLRGWKCIDSV
                     FEQWAGGAGQQCRLLVGMQKLAQDELRDALSLLPKEDEVSNQLVIRMKRKLAEDFRNQ
                     LTFGAPTDADEAGLRRLAAQLKSGKVVVKLFLRHTLHAKLYLCFRDDPNNPITGFLGS
                     SNLTLSGLSNQGELNIDVLDHDATQKLAQWFEDRWNDRWCVDITKELIQVIEESWAGL
                     EALPPYMIYVKMAYHLAEEARAGLSEFQIPADMRDTLLDFQAAAVRMAAGKLEKRGGV
                     VLGDVVGLGKTLMATALARVFQDPPRSLETLIICPKNLKGMWEDYAHRYRLIAKVESI
                     TQAQNVLPELRRYRVVIIDESHNLRNREGRRWAVIRDYLSRNGSKVILLSATPYNKAY
                     LDLANQLRLFMEPDATVPIRPEEYLRQECGGRVEEFTRRHQCPVNCLAAFEKSEHADD
                     WREMMRLYMVRRTRSFVEANYAWTPCSKCDGILSTVQDACPKCGVAKDTKARRFLLLE
                     GGTRFHFPKRQPKKLEFRIREKSPDDQFAKLYSGTVVDTIRILHLPRYGLGNYLRPTP
                     DHPPSQDEAEIMRNLSRAGQRLTGFCRTNLFKRLESSGHSFLLSLRRHILRNFIALHA
                     IENGLPLPIGTQDSALFDTRDEDSDSESNLFGQDAENAPPELTEINAASLDDFKTVAG
                     QSYETLRQHHARNFDWLRPDLFVADLAKHLLQDAQRLFSVLEMSGVWQPERDEKLAEL
                     HKLLTKTHAAQKVLIFSQFSDTVNYLAAQLKDKGVTRLAAVSGGTEDPSVFTRRFSPD
                     SNKVRDKVSAADELRILIATDVLSEGQNLQDAAIIVNFDLPWAIIRLIQRAGRVDRIG
                     QKAEEILCYSFIPADGVERLIRLRARVRQRLQENAEVVGTDETFFEDERHDGLIHDLF
                     TEKSGILDDPEDGEVDLASLAWQIWKNASDADPALRKTIPDLANVVFSTKALAAIPPR
                     PAASTSGVQPSTGVMVYVRTADGNDALAWVDEQGSTVTESQHEILRAAECEPSTPAQP
                     RLANHHALVQQAVTGIQQEHFTTGGQLGKPGGARRRTYERLKDHAAEVKGTLFDIKPL
                     HRAIDALYAAPLTDASRDLLNRELRAGVSDDKLAELVLTLHEEDRLCIPLEEAHAREP
                     RIICSLGIRE"
     gene            complement(34024..35433)
                     /locus_tag="PLP58_02570"
     CDS             complement(34024..35433)
                     /locus_tag="PLP58_02570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ07437.1"
                     /translation="MKAIFILSLLGGLCMAAEPIHIDSRRELFVDEHLIASKSGVELK
                     MHKPQAQDVALVCDAPWEGNTSGYFTLLQDGDLYRCYYRGSHHGEPGGRPGHSGVTCY
                     AESRDGITWTKPKLGIFEFGGSKDNNITHMGDGCGTFAPFLDTNPNCPPESRYKALAT
                     MGNDVERKKNPALQAYHSADGLRWSLMREKPVITAGSFDSQNTAFYDNELGAYRAYWR
                     YFTGGYTDERGWKPSGVRAIRTATSKDFLTWENQADLAYGKDAPKVQLYTNAVRPYAR
                     APHLLLGFPTRYQPKGSQVEPVLMTSRDGVNFKRWEEPLIPITAPKDRDWNRSNYMTI
                     GVLALPGRPDELSVYASEAYYQGPGSRIRRFTFRVDGFVSASSKQGEIITKPLIFAGS
                     KLQLNLECQIETRVEIQDTEGKAIPGFTLEDCTPMVGGPTEQTVTWKGGSLAHLAGKP
                     VRLRFHLIEADLFALQFVP"
     gene            complement(35430..36680)
                     /locus_tag="PLP58_02575"
     CDS             complement(35430..36680)
                     /locus_tag="PLP58_02575"
                     /inference="COORDINATES: protein motif:HMM:NF024771.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mandelate racemase/muconate lactonizing enzyme
                     family protein"
                     /protein_id="HRJ07438.1"
                     /translation="MKITRVRTLLSTAPQRDIFMKSRVRRSAAFVLIETDTELTGLGE
                     TYAGYFVPEMVPEIVRFYEPILVGQSPLDVDVLSRRMFTAGKFWARVGLGSIVLSGIE
                     SALLDLKGKMLGVPVYELLGGRCHDTLPCYATGGTSPHDRGELEGKVAQYLSLGFHAV
                     KLGAGTYRGPGQPIVGSRTPQEARDVEVGKVEFLRERFGDSFQLNLDAHMDFLGESDH
                     VWNLATAQFVLKALEAYPIGFFEEALPYTDMRAYGELRRSTTIPVAGGETLTSLEEWR
                     DWLEHKPFALAQLDASFMGGLMNFIKIARLCELQGVSIATHAWSATPGVAANLHAAFA
                     CRNTAVCEMAAYNPTRPELNTHITAPLLTDLWIEPPVIENGRIRLADTPGLGVRLPEG
                     FLDRHPFEPGSGEFSSVTGKILQP"
     gene            complement(36677..37687)
                     /locus_tag="PLP58_02580"
     CDS             complement(36677..37687)
                     /locus_tag="PLP58_02580"
                     /inference="COORDINATES: protein motif:HMM:NF014841.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-2-hydroxyacid dehydrogenase"
                     /protein_id="HRJ07439.1"
                     /translation="MKPLCLTTALPFTECPPGSEPEVAGLEMRVLRNVKQGDPWPVDD
                     AGRVTAAFCTHPPPNLAAFKNLRWLQIESAGFSHLFPFRLAEKTIIVTNARGIFDCPI
                     AEWNIAMMINLVRDLRTLIRHQDAHVWQRSAQFTGEVRGKTAGIWGYGGIGRETARLA
                     KALGMRVHVLSRSGVKSRAGSTCIAGTGDPDGSLPDRYFTQGEKVEFLSGLDFLVLAL
                     PLTKATDGLIGEAELRSLPKGAFVLNPARGPLIQEQALLTTLSDGHLGGAALDTHYAY
                     PLPAEHPLWDFSHVILTPHISGTTFSPRFSAGLLDIFRQNALRFIAGEPLLNLIPPAD
                     LE"
     gene            complement(37684..38565)
                     /locus_tag="PLP58_02585"
     CDS             complement(37684..38565)
                     /locus_tag="PLP58_02585"
                     /inference="COORDINATES: protein motif:HMM:NF019474.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HRJ07440.1"
                     /translation="MKIHSVAKTAIILISCSAGSAPAQTALPPATFAKVSYGTHERNT
                     LDFWRAKSAKPAPLLIFIHGGGWAGGGKEDLPPMLLDAMLVAGVSVASINYRYSTQAI
                     LPAPVHDAARAVQFLREKAAEWHLDPARFAAYGISAGATTSLWLAYHDDLGPPSTRLR
                     AVVALSPQPSLEPRILRQWAGDEVLKHPLMARAVGVKTGGDVLKPKPEWVKLWREFSA
                     ITHVSKDDPPVMVQHPRFDPLPAPNPGSAIHHAIFGVKLKERCDAAGATCILRLQDRP
                     GDTLEPEAFLIEQLKMP"
     gene            complement(38562..39485)
                     /locus_tag="PLP58_02590"
     CDS             complement(38562..39485)
                     /locus_tag="PLP58_02590"
                     /EC_number="4.1.3.3"
                     /EC_number="4.2.1.41"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: protein motif:HMM:NF012904.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009436 - glyoxylate catabolic process
                     [Evidence IEA];
                     GO_process: GO:0071704 - organic substance metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HRJ07441.1"
                     /translation="MKPAKRHPRCILATCCVPWNERFEFEEDIFRRSVRHQIASGIRD
                     LYIFGTAGEGYAVTEKQFDEITRVFLDETTQGGAKAMIGLISQSLPAIIERIERAHDT
                     GARRFQISLPSWGVLNDAEVEIFFRETCGRFSDCAFLHYNLMRVGRILTGTEYGRLAA
                     EHENLVATKNSTADETRLRSLLTDAPQLQHFITEPGFAKAALMGECGFLISFTSTNLK
                     MAAEFFQAGCARDEARLEAISAVFPGLIAAFKEAVGASAHMDGAFDKMFCRIHNPEFP
                     LRLLPPYAGVSDAVFQRYLGALQHNHPEWLP"
     gene            complement(39482..41014)
                     /locus_tag="PLP58_02595"
     CDS             complement(39482..41014)
                     /locus_tag="PLP58_02595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ07442.1"
                     /translation="MRYNLLIPLIFASAGSAAERTLLMADDHDILYRPGTTRVLEKPV
                     RHPQNPLITEDKPWEMAIAWTSVHRDKATGRYQMWYQAYAGKRAQLKTHECVVCYAES
                     ADGISFTKPDLGLFDFNGDKKTNIVLIGSGLHGDRYCNSVSVDPRERDAAKRYKMLYY
                     DWAKNAKGEIIAGLHLAFSPDGIRWTKHPEAPLYATSFGGKKMQPPFAGESPYHETSL
                     PDGRVRRQLLLPMTMSDAADPLFDPKRGVWAIYGKMWIHGPDGGLGWKHGMGRVESAD
                     LIHWSKPQLVCAPDEHDGPLEFHTSPVFLRHDRYFSLNQILNRPDGGVMDIELMLSRD
                     GIEWERPFRQNLWLKRSPGEQFDSGTLATNADIVIEGREMRFYYGAYSSGATGGGINI
                     TGDQQKSGVGLVTLPLDRFAAVTAADKGQITLKPLDLKGRTEVYVNANASEGAVWAEI
                     LDEDGFRLPGFDKANCMPLKKDAMRYRFAWKDKKLADLPPGKHHIRLHLHKASVYAIT
                     LR"
     gene            complement(41011..42177)
                     /locus_tag="PLP58_02600"
     CDS             complement(41011..42177)
                     /locus_tag="PLP58_02600"
                     /inference="COORDINATES: protein motif:HMM:NF025330.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kelch repeat-containing protein"
                     /protein_id="HRJ07443.1"
                     /translation="MKKKAAVLNAMLVLTGICAAQEDPRLAERRLLKEQTAQRAGVER
                     ALSREMPDELQALPVFGGIEWTVQAMPFIAPGPHGGVSGGGMAVVDGKIYYAGGFIPA
                     GDGTEEAGNRTSRWAHVYDPQTDEWTRLPDMPARREYTRAIATDDAFFIIGGFAQGRP
                     GLPAAEVFRLDVRRAPLQWQSIAPLDVPRSHTAADRVGGRLIVAGGNRYDIAEKGYSA
                     KTIQCVTEVLDLALPEKGWRQAAPIPGAPRGWCASAVLDGQFYMLGGVTWTPAGRVRL
                     TECLRFDPAQNTWTRLADFPVPISGWEADVFAGRYLITVGGACSRWNDAAFVYDTRED
                     RWLRIASPLPPGGLFNDPGVCIIGDTIFVAGGEGPGGSHFEYFLIGKIKPAREP"
     gene            complement(42174..43748)
                     /locus_tag="PLP58_02605"
     CDS             complement(42174..43748)
                     /locus_tag="PLP58_02605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ07444.1"
                     /translation="MPLYLALPQAAVIGLALVVSTLRAEELFDESKAVTLFAFDDVSI
                     PHTQNLRLEMRQPVRHEANPVLRRGAAGAPDAMGVQFYGSILKENGKYRLWYVAFDDD
                     TENKVASARWRAAYAESVDGLTWTKPDLGLVEHRGSTKNNLLDMGGQSWGFVNLKVIR
                     DDADPDPERRYKMTTHVYYRHNTRLGTLLPFVSADGLRWKPVKEVKPLKAELRKEDLF
                     IPGFHFEPCGGLYQWDGQFYISGQNALPGTHLYQGRVSRTYRSTDFVNWSPTSSIAFV
                     RAAQHDYLGPGRSREGEQNHEGISVWNRRNVLLGVYGRWHGAEEWKDVRIDLGFVMSN
                     DGLHFREPKHEWTFIGRGNDGEWDQGGLIQGQGFENIGGQTFIYYGAWDPRPTGGPPQ
                     PRGGVGVALLPRDRFADLMMDTSTEGPGDYQLPQVTAEFVTASLDVKTPRFFINADGL
                     GAEAVLRLELLDHLEKPVPGCSGENAARVTRGGFQVPVEWKTAAPLPDRVRVRGVFEG
                     KNRAAIRLSAIYLLQP"
     gene            complement(43896..44837)
                     /locus_tag="PLP58_02610"
     CDS             complement(43896..44837)
                     /locus_tag="PLP58_02610"
                     /inference="COORDINATES: protein motif:HMM:NF013429.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HRJ07445.1"
                     /translation="MTRAVSPAGQQLSLSTCWNSHRHTEGKALAVEARELGFEWIEVS
                     HGTKISLLPGLLDAVAEGIVRVSSLHNFCPSPVEVMMDAPDVYEFTSDKEWERERAVS
                     LTRKTIEMAARFGADRVVVHLGRVSMPAITATLEKLVHAGGLYSRAFCALKLRLVAMR
                     AKRAPVRLERARAALAALLSDCEKHRVRLGIETRSHHEQIPNQKEMRALLDEHEGCQW
                     IGAWHDFGHVQRQANLALLNHGEFLTQTAPRIIGCHIHDVRWPARDHRVPLSTGGVDF
                     ANLLQLVPAETPLVWELSPSQTAGDLRSALARWQGCE"
     gene            complement(44834..>45703)
                     /locus_tag="PLP58_02615"
     CDS             complement(44834..>45703)
                     /locus_tag="PLP58_02615"
                     /inference="COORDINATES: protein motif:HMM:NF018038.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ThuA domain-containing protein"
                     /protein_id="HRJ07446.1"
                     /translation="SHPPGMHEFRAGSMLLEKCLAGVTNLIVDRHEMGWVRDESTFAD
                     ADAVVIYADGGKGHPAVQEGRLETLRGLMAKGMGFGVMHYGVEVVPDLGGREFLEWVG
                     GHYENSFSCNPIWEPRFDKLTEHPVTRGVEPFQIEDEWYFNMRFRPAFGDGITAARDG
                     AASFTPILVAAPSDATRDGPYVYPKGPYPHIQAEKGAQEAMMWCVERADGGRGFGFTG
                     GHFHRNWGNDGFRKTILNALLWVAKAGVPSGGVESFVSREDLVENLDPKPAPKAKKAA
                     LETPKVFQRLANR"
BASE COUNT         8434 a        13636 c        14951 g         8682 t
ORIGIN      
        1 ggcctgctcc tcatgcggag ccgcgagccg gtcttcgacg acactaaata tacggcgggc
       61 caggtcttcg tgcccggcct ggaaaaatcc gactgcggtt tccaccagca cttcggcctt
      121 gggcacgttc gagatggcca gcacctcaat gccgctctcc acaagcgcgg tgcccagcgc
      181 ctgctggcgc gcatccgtgg aggcgagcct ttccaacgcc tggccgaaca aacggcgtga
      241 cacctccagc aatgcgggga cgggcttccc gcgtgttttg gcatcctggc cggagcgcag
      301 gcgggccagg tcaaagacgc cggttttcgc ggactcgagc gtcatgagcg ccgcgcccga
      361 ggcgaagcgt gtctccggat cgcgaatgct ctcccaccag acacgggtga catctccctc
      421 cagctccagc aacgctcccg catggaccag ccgggccagc accagctcag cctgccaggg
      481 tttgatcatg cctgtcatgc ccgcggcgag catcaccagc ccgcgtgcct gcccgcgatc
      541 tttgcccgct tctttttccg cgatctccag caggagcaag gcggcacgat aatccgtgag
      601 acgcgccgcc agctcgcggg cggtggcggt tctgccctgg cggacggctt gacgaccgca
      661 atcatgcagc agccggtttt gtgtctcggt gtcgccgctg accagggagg cggcccgtac
      721 agccaggctc agtggatcgt cagcggaggc ggagggcaaa tccatgccgt ctggctgcag
      781 gcgcacactc tggcgaacct cgggaggcag cccctccagc gctttttcga tgccggcctg
      841 gtcctgcccg cgcgcatgag ctgcggcggc ttgaaaaagc aggaccgaaa gggcggcgct
      901 ggcagcggga cgaagggaaa atgaaatgaa gctcatgaaa cggaaaaggt gggacgatgc
      961 ctggcaccgt cccaccgggg gtattcaaag ggttcgaggt cgcttacagg ccatagactc
     1021 ggtagagaac agtgtcgtcc tcatcactgg tggtgtcagt gtgaacgacg atgccatcgg
     1081 tgccgaccag aggctcggtg gtgagcacgg cccacctttt gccatcggcg gatttttcga
     1141 ccacatagcg cacaccaccc acggactgga agctgaccgt cggattgccg ccagacacgc
     1201 caagactgat ttccggacgg acgagattgt catccacgaa ggcttcgagg gcgtgaagcg
     1261 aggtccagcc gaacatctgg ttgttgtcgc gcatcgagcc ggcgaaggcc gcgttgacaa
     1321 gcccgcccgg caggcccacg gcgaagggca cattgccgta agcggcggcg gcgggatcaa
     1381 ggcggggatc aagtccgttc ttggtgtaga gcgggcacgg caccaccggg ttcgagacgg
     1441 ccgagaagcg ccacggagtg cggatctggg agacacctgg gaaggtgagc gcgttgacct
     1501 ggctgagaat cgggatgact accccgccga ctgtggcggt ggtcgcgccc acgctcactg
     1561 tgctgctggc gggcagaggg ttgccagccg tggccgtggc gcggtaggca aagatgttgc
     1621 tccaggtcag cagggtggtg atggcgccgc cgccggtctc cgccgcgcgg ctgacggcgt
     1681 tgacgttttc gatgacgccg ttgtgcatcg ccacatgggc ctccagcttg gggttgaggc
     1741 cctcgaactt cgtgccgccg gtgagcatgg tgaaattgta aaggctgatg tccgtcatcg
     1801 gcaggcggtc gccgttgtca ggctcgccgc cgtcccactc catgagcttg tcatactggt
     1861 agtcggagcc ggactcgatc tggccgatgg tgccgttgct gccggtgtgg ccgctgcgca
     1921 gggcgccgcc ggaggtgttg atggtgccct ggatgacggt ccagaactga tggtcgccgc
     1981 gaaagccctc gtcaccgtcg aaagcgtcgt cctgattggc gctggagaag aggtggcggg
     2041 tgtcgtgctt gccgccgaac cactcaaagc cgtcgtcctt gttctggtaa acttcgcaat
     2101 gctcgaaggt gaagcccgtg ccgacaccgc agatggtgat gccattgatc tcgttgccga
     2161 cggcggcggc gccgatcacg aatccgccgt agcggttcac cacatagcgc atcacaccgc
     2221 tgtcgtcatc gtcgtccgtg ccgccgtaaa tggtaagctc gctgttgagc acgttgctgc
     2281 cggagccgct ggatagaccc tcggggtagt ccgtgccgac acccgcgttc tgaatgcggc
     2341 ggaagttttc cgtcaggctg tcgccgtggg cgtcccagat gccgttggcg tccgcatccg
     2401 tgttgccggt gttggcggcg acgtggccgc ggccgcaaag gatgatgccg ccccattgac
     2461 cactcttggc gaagccgttg ttgccgctgg ggccgccagg gctgtagtcg ttgttggcga
     2521 gcgctgtcac gtcaatgacg cggccaagca ggttggtgac cgaagcgggg accgtggcgg
     2581 tgccgccggg gacgttggga tcatcaatcg aggtgaagat gatgggggcg tccacggtgc
     2641 cgttggcgac cagcttgctg ccccgcgcaa tgacgaggga gccgggctct tccgcaatgt
     2701 cggactccaa agcgcgcagg ccgcggatga tggtgcccgg ctcgatggtg agcttggcgc
     2761 cgttggagac gatgatgacg cgtccgagaa tgtatacgtt gtcgcgagtc cagcgggtgt
     2821 cggtggtgat gcgattatca gtcgccgaac cgacgacgat gaagtcggcc cgggcctcgt
     2881 tgagcgtccc ggcggcaaac gccgcggcta ggaaggtggc tttgatcgtg tgtttcatga
     2941 tatgtttcaa aatgcggggt cttgtgcgtt gctcgaacct gtgctcatca aggaattccg
     3001 agagtaacca aaccggactg caccgggctg gtggtggtgc caggaacctg ggtgagcatg
     3061 aaccagccga atcctcccca ggatcccatt tccccgccat tgcggatcac atgcccctgg
     3121 aacttcacgg tcttgttcgc caccccgtca taggtgaagg tgccgctgaa gatgcctttg
     3181 gcggcgttca cgaccagctt ggtaagccgc ggcggattgg cggtgttgat ctggaccttg
     3241 ttgcccgcct ggagagtgac attgatgtca gggcgcgcgg gtgtgttggc ggggctgaga
     3301 cccgcgtcac tgaattcgag cagcgcggct gtgtccgcgg caagatcaag gatgcggcgg
     3361 tttttgtcgg tgatggcgta gcggccaccg gcgatcgtga ggtcgcccga ggccaggttg
     3421 aaccctccgg cataagcgcg ggtcttggtg ctggtattcg cagggcgcct ccaggtgagc
     3481 gtgccgccga cggtgtcctt gacgaccaca tcaccaggat tgagctggag gttgccgacg
     3541 atggagcctc tcagcggcgt gtagagcaga ttgaaaagca gcacctggcc ggtggggccg
     3601 acaaaggtgg agccggtgat cttctcgccc tcgccagtgg tgccgacgat ggcaacggag
     3661 ccatccggct tgatcgtgag ggtggccagg gtggcgccct tgggccgggt ggcggagtcg
     3721 ccgctggcgg tgacggccac attgtaaatg ccggggaagg tggccgtggg gttcttggag
     3781 tcgaacttgc ggcgccagcc tgtgaccgtg gtggagcgga tcccggtctg accgggaaca
     3841 aggaaggcat cccgcaagac agcgctggag agcaccttgc gcgccgtgtc accgttatcc
     3901 ggcgtctgga tggtgaagac aaactccagc ggaacgaagc tgagaccggg gcggcggatc
     3961 aggatggtgg ccgtgggcga ggccgcgccg gggttgttaa ggaaaccagt gaatttgatg
     4021 ggtttggcat cccccaggaa aaggtttcct gagagggagc caagcgtggt gatcagcatg
     4081 tcaatgcggc cacccagatt gttggtgtga gagtacccgc ccgagaacgt ggagcgcgcg
     4141 acccaggcgg tgtaagcgcc gttcaggttg gccaggatgt tgttcacgag aagcgtggca
     4201 ggacgcgcaa gcttgtcctt gccattcgtg atggtgattg tgaggttgaa gggaaccagc
     4261 ggatccttgt tcaacgccat ggggcgacca ctcacaaacc cggtctgagg atggcaaacc
     4321 atgcccttgg gcaagccgct gaccgcgaaa agggaggctc cgttcgcagg tatctgaaaa
     4381 ggagtgtaat cctggccgag ggtggcgggc ggcagaacga tgggattgtc aacggtggtg
     4441 aatgtgggcg cgccattgaa gacggagaga ttgaaatcac cagattcaag cgagtcaaag
     4501 ccggccacgg cattgccagc gaagatttgg caggtgtaga cgtcgccatc gttaccgtta
     4561 gtgagacctt tgatgccgag acttttggtg gtgacgccgg agaactggcc gccgttcgtg
     4621 agatccacac cattgcgttt ccactggaac cgggcggctt tgccgaaata gatggcatcg
     4681 attttgaccg ttccaccgac gggtcccccg atttcgcgcg tctggacgtc aatgaccacc
     4741 acttcagcca cgaggctgtt tgtggagcca ccgccctgga tcgcagcggc attggtcgcc
     4801 acgcaaatgt agggaccggc atcatccggg cgggcattgg caatttcgat ctgcgggtct
     4861 gtggcacccg ggaggctggc tttgtttttc cactggtagt tgactccgtt agggctgaca
     4921 gcctgcgcgt tcaaagtgat ggcggatcct atggccacga catcggacat gggctgcatg
     4981 gtgaaggtgg gtttgatccc aagcacctgc aacggcacga cgttggtggt gacgaccaca
     5041 gtgtcatcgg tgacaacgca gtcataattt cccgtgttct tcgtcgtgac agagggaatg
     5101 accaatgtct cgctgttcgc cttggagatg tttttcccgt tcagacgcca ctggtatttg
     5161 atgccgttga atgacgaatc ggcaatggct gtgaacgtca ctgcggtgcc ttggaagatc
     5221 acctcatcct ccggcgtgcg ggaggcggtg gcatcggtaa cacgatagtc atcacccacg
     5281 gtcgtatggg cgttggccgc gccgccgatg tcggcgccgt ccgccagatc gccgacatgg
     5341 gccacaaggt ccatgacacc cgtgtcgggg ctgatgaagc tgtggttcgg aaagatgcca
     5401 tccggagtga caaaggtctg gctggtggaa aagatcgctt gctggggagc agtggccgcg
     5461 gtcgcgccat tcaatatcca aacatggatc tttttgccgt ccatggatcc cgtttcgtag
     5521 tcgaagtcga gatcaaaaaa gccgcgctgg ccatccacaa acagaggggc tgtggattgc
     5581 gccacctggg tgaaatcggc attgagctgg gcaaatgtct tgccgctgaa atcataggcg
     5641 tcaggaaagt agccgaagcg aactgtggca gtgctgatgc ccaccaggcc gttgctgggg
     5701 gctatcccgt tgccgaggtt gctggcgaaa agcctggccg gagccgcctg gacagctcct
     5761 tgcaggccca gcatcaaagc ggtggttaga agaatttttt tcatgagtgg agtcaggttg
     5821 agatggggtg cggggttcag tcagcgaaag gctcggtcag ggcgaatccg gcggtgccgg
     5881 cctgctcttt gaacaagaga gctttggcgg gccggatgag cgcgccgcca gcctctgtca
     5941 cgctgtcccc cagcgcgcgc cagccggtgc ctccagggcc gccagttttg aagtagtatc
     6001 cggtgagcac tccgccctcc tccaggtaaa cgatgtccgc cacgccggga ccggtgccgc
     6061 cggtgatgtt tgactggatg tcactggcgg aaaggctggt ggcaatgagg aagggattgt
     6121 tcacgatgtt gtgaccagcg gccacatcgg cggcgtggcg cgcaggaagc gtgtcaccct
     6181 ggaggcgcaa ggtacgatcc ggaccggcaa cccgtgtgat catgagaccg ctgccaggct
     6241 tgacgaccgt gttttcctgg tcggcaccgc cgggatcact ggcgagacgc catttgccgg
     6301 cggtggtgtt aaagaaatag gcgaccagga ctccggcgtc ggtcagataa accttgtccg
     6361 cagcgcccgg agcggcggcg ccagtgaggc cggtctcatt ggccgcgcca agcaggccgc
     6421 ccagagtggc cagttggtag atcacaaact catcccccac gttcggcccg gtggagagcg
     6481 cggcggtgac gacctggccg gcctgcgcgg tgatcgtggc cgacttgcca taggcattgt
     6541 tgcccgcggg attggcccgg ctgacgattt tcagcgcatg aggcgctgcg agcacatcag
     6601 gcagagcggc cggactcgac accagcgtga gatcgccgcc agcgacggct ccgaccgtgc
     6661 cggaggcgac gatcttcaca ttagaaatgc caaccaggga ggttgctcct ccaggcaggg
     6721 cgacggtgac tgtgcccgcg gtgcggctca tggggagttt gtccgcctgg ccggaagctt
     6781 gtgtggcacc gagtgccaga agcgtcacca gggcgagagt tgtgtgggat ggtctgatca
     6841 tgatgaagaa aggtggtttg cgaggcggcg agccgcgcgt cctcacaccg ccgatttgaa
     6901 cccactcacc cgcattttcc aaaacctcgc tggcaacact tttgtcacct tgagattgcg
     6961 acttgcgcgt ccattcacca cgccagcttg actcttgcgc gggcaagacg aatcacgtca
     7021 caattccgtc actcggcgcg atcgcccgga gtcactttcc cgcagcttcg gaagcctccg
     7081 gcccgactgt ctgacgcgcg tcatttggcg gggtcttgct cgtgatcacc gaggcgagct
     7141 tgtcacgaag agggctgccc tccatggcga gtccggggaa tttcaaagcc agctcgcgat
     7201 agtaaatggc ggcggctttg gcatcgccgc gcgcctcgta ataggcggcg agggaggaaa
     7261 gctcgcgctc ctcggccacg cgcagctcgg ccatgctggc gcgcgcctgg gcggtcttct
     7321 cgctctccgg gaagcgggcc agaaaccagg tcatgaggac cgccgcgcgc tcgcggtcct
     7381 tgggagctgc gccgcgcatt tccttccaat gtttaaaggc gatttccgcc gcctgatagc
     7441 cggcgtcgtc cgccagctca tgcgccggat gcttctccac gaagtcccca taagccgcgc
     7501 gcgcctcctc gatgcggccc gctttttcca aagccacgcc cagccagtaa tgcgcctgga
     7561 caccggtctc ggagtgcggg ccattcaaaa tgacgcgcgc cagcatggcg atgatgtcct
     7621 cctgcggcgc catgtccgct tttttcaccg gctccaggga gcgccgctgg ccgcgggcca
     7681 ggccgagcag gcggagcgcg agccggagct gctcctcgtg cgcgcgtgtg aaatggccgg
     7741 gctggcggtt caccaactgg tccagcgcct caaaagccgc gttgtgctcg gcgtgcttct
     7801 gatgaatggc cgcagtgtgc cacaaggcct gcgcggacca ctctgtccgc gggtattttt
     7861 tctggatgac cgcgcagcgc tccagggcgc cccggtcatc tccggcggcc agggcttttt
     7921 gcgcgcgttg cagaagcaag gcggcggtct catccgcgcg cgcgtcctgg tcctgggcat
     7981 gaatacccat cgcagccagc aaacaaagca gccccgcttt cacggggctg gtgaaaaaat
     8041 ggcgcgccgc ccgcatgacc ttactcgctg gaggtggagg cgctgtccag cgtcggcagg
     8101 ggcaggagtc cgccgctgcc ggaggcaggc atcgggggcg gcagcgtgga cggcttgtaa
     8161 acgcgcgcga gttgctcgcc ggattttttg ccggccgtga tcctgccgag caccacatgc
     8221 acgccgcgcg atttgccgag ggtgtttttc acggtggcct cggccccggg gtagagattt
     8281 tcgacgaggc cggagttcgg ccccagacag tcgagatgcg gcacggaggc gtcgtcggcc
     8341 agtgtgtagt caaagctcac cacttgctgg ccgatgtttc ggaacttgac gctgtgctca
     8401 agcgtctcct cgtccacgat ctcgctgacg agcctgtagg tgacgggcga gtcgccgatc
     8461 acgacgggat cgctcgtcat cgttttgcag gaggacaggc cggcgagaat cgctgcggcc
     8521 agaatcagtg aggtgaagcg cgggatcatt tggacggaag tctgaagtga gtgaatggag
     8581 gtgatcaata gcgataacgg aagccgacaa ggtagccatc gttgcggaag ccgcgcaggt
     8641 catcgagatc gcccatgtag gttttgcggt attcggcgaa cacagagtag cgttcctccc
     8701 agctccactc gaggccggcg cgccagttcc agacattgat gaactcgtca atcgagaagg
     8761 gcacagcctc gccgggctct gtggtgccgc cctgaacctg gtccgccgac ttggcggtgg
     8821 aattgcggaa ccacacctgg cctccgccaa gaccgccgcc gacataaggt ttgaagcctc
     8881 cccaaacctt gccgatgcgc gcgcggtagc ggtagaggtc cagcgcgaag gtgccgttca
     8941 agctgaagat cagcgaattc atgtccgact ggtattctgc gatctggtcg ttgttcagat
     9001 tcgcggcagg cacgctgccc tgcagggtcg tggaggtgaa ggttgcctcg gcggaaagcg
     9061 agaactccac cggcacccgg cgcatgttcc aggtcttgcc cacctccaca ctgaaaatgg
     9121 cgctgccgtc aatgtcctcg accgggaaat tccgctcccc caccttggcc gtgccggttt
     9181 gaccaagtgt ctcaccagcc atcacgctga caaaagggcc aaatttccgt ttctccttgg
     9241 cctcacccac agcgccaagg gattttaact caagctcagc agcgccactg gggtgcaaac
     9301 aagcggcggc aatggccagg attaaaaaag cgggcgggag gatgacacgg agacgcatga
     9361 tgcaaaaaaa cgagtttcaa ataccgccgc cgacttggtc ctgaaatgag ttccatgtga
     9421 agaattcgtc acacgcgcga cgcggcagcc cgtgatccgc ccattatgag tgagtcatgc
     9481 ctcacaagct cactgaccgc cgcatcaccg cctcccctgc cctgcccgcc ctaggcgtgc
     9541 tgctcgcggg cgtcatgatg gcggctggcg gggagaagga caaagccgcc cagccggatg
     9601 ccgtgctttt tcctctggct ccgcgcgcgc aacccgcgca accgccactg cccagggaag
     9661 gcttcccaat ggactccggg aattaccaaa acccgctcat cattttcacg gaaggaccag
     9721 cctcaccccc gcttccggac gagatgaacc ccgacctttt gcctccgctc ctgcccatgc
     9781 ttgaggagga cgcggctccc gctccgcctc cgactgccgc gcaactccgc ctgatggctt
     9841 tgggtgaaaa agtcgctgcc gccgtggttg gcatccgcgt ttgggacagc ctcggcgtgc
     9901 agcttgcgtc cggcattggc tcctatgtca ccaccgacgg catcctgctc accgacgccg
     9961 gcctgctgca tcctgagatc gcggaaaaga ccggctacat caccctcgta cgcgctgacg
    10021 gcaccagcac acgcgccgcg ggtttttatc tcgtggacca ggccagcggc gtggcgctgc
    10081 tgcaaagcga ggactttgac accacaccga tcgagctggc tccaactgcg gacttcagcc
    10141 gggaacgcgc cgcccatgtg ctggccgtct cggaaaaacg cggcctcgtc ctcgccgaag
    10201 cccgcgtcca ggcggacccg gccatcaccg ccctcggctg gctgaacgtg cgcggtgacg
    10261 attccccggg agccgccggc tcgcccgtgc tggatgagga aggccaagcc atcgccatca
    10321 tcggcatggt cgtgcctttg aaggagtgga aaaacttcgc cctgccagtg gatgcggccg
    10381 cgctcgcgct gcgccagaag cgcgcgccgc tgcgcccttt gagcgagctg ccacgcacgc
    10441 ccggacttcg ccaggtcgtg caggatccag cctttgtgga ggccttccag accctggagc
    10501 aaaagcgact cgaacccgcc atgcgcaagc ttgttcaact cacccgcaaa tacccccgca
    10561 gcgccgaatg ctgggcattg ctcggtctcg ccgcctcccg cctcggagcc gggccggaag
    10621 ccgtgaactg ccagcgcaaa gccgtggccc tcgaccccaa agccggcctc tactggcacc
    10681 agctcgcctt cgccaaaatg cgcgagtctc ccagcccggg cattttgact ggagaggact
    10741 gcgaagccct ctcgcgcgcg gtcgagcagc gcccggacga cgccctggcc tggctgctgc
    10801 tggcgagcca ctttgcccgc gcgggggatc tcgaccaggc ggatgccgcg ctgcgccgtg
    10861 tcaccgtgct ctcccccgcc tacgcgcagg cccattacct ccaggcgtat gtgcgcgggc
    10921 ggaaaggcga ctacgccggc gccgaaacct ccatccagcg cgccctccaa cttgatccga
    10981 aacacgccga agcctggtat taccagggtc tcctgcatga caaaaagggc gccccagcct
    11041 ccgccgccaa agcctggcgc aacaccgtcc gcctccgtcc cgaccacccg caggcatggt
    11101 tgaacctggc ccacgcgcac aaaaaagccg gacaagccac tgaagcccgc caagccatcc
    11161 tggagcatca aaaagtccgc tcagcagccg cgcattgaat gtcaggcgca gccctcactc
    11221 cgcgcggcgc gacagcaaag aatcattcgc cagccggcga tccatgaggc ccggacacgc
    11281 gccgcttgtc atccgggtca ttggcgcgtg tgtaacaatc catgcgcgtg tccgccgttt
    11341 tccagccggc ttgcagccgc gcatccgccg ctccggagac cgtgcccgga gctgaggaga
    11401 ggctttcatc cgtggctcgg cgcatctccc accagcactg gccgcagtgg ttgtgctcgt
    11461 ggtccaccac aaagccggcc tcgcgcatca gcgggccgat ccagcccatg cagtggtcgc
    11521 agtagtccgg gtattgctcc aggccgttgc gcagcaaaaa gcccttcgag gggcagtggt
    11581 gcatgtctat gcggaacacg gcctcagttt tcgtcacctc ccactcgccg ccctcctcgc
    11641 ccaaagtgtg gccccagtat tcctccatgc cgtcaaaacc accggccatg atcaatgcct
    11701 ccgcgtgccg ctgcgagtcc tggcggatgg cctcgtccca gtaggcgcgc accaaatcgt
    11761 ggccgcccag cttttccagc cagtcaaaag tccactcata gtggccgcag aagtcataga
    11821 cgccgatcat tttgcccatc aatacggagt ccggagttga aaagcacgct tgaatcccgg
    11881 acggggaaaa aaatcgccct tggcatgacc tgcgcgggat gtaatctggc ggcgggtcgg
    11941 ccggaggggt cgcccgtcgg tggtgctccg gcatcatgcg gacgggagga aagtccggac
    12001 accgcaaggc agcatgccac gcgcaagcgt gggggcggcg gagaaatccg gcgtcacgga
    12061 aagtgtcaca gaaaacaaac cgccgtccgg caacgggcgg caagggtgaa atggcggggt
    12121 aagagcccac cgctccaacc gcgaggaagg aggcacgaca aaccccatgc ggtgcaagac
    12181 agaacaggag aaaggcccgc ctggccgcaa acctgtccgc tccggcggac gggtggatga
    12241 ctccgggtaa tagtcgcacc ccgcgcgagc ggaggccggc gccgcaaggc gctggcagac
    12301 aaatgacctc accggaggcc gcaaggccga aggaacagaa tccggcttat gaaggccggc
    12361 ccaccttttt ctcaaatcaa agcgccaccc gatgtgatgc ctcaagtgca gcacgggcag
    12421 ggctgcaccc agtggccggg ggcgacttcc ttgagcgcca gatccatgcg cgtgctttcc
    12481 tcccacttcg gatgcttcat gcggtggccg aaggcgcagc cggcgggcgg gttgatgggg
    12541 ctggggacat cgccctgcag caactggcgc ttccgtcttt tggcggggtc cggcacgggg
    12601 atggcatcca tcagcgcctt ggtgtaggcg tggcgcggat ttcgataaag ttcctccgac
    12661 ggggcgagtt cgacgatttt tccgagatac atcaccgcca cgcggtcgct catgtgcttc
    12721 accacactca agccatgggc aatgaaaagg tagctcaggc ccatctcacg ctggaggtcg
    12781 agcagcaggt tcagcacctg gctttgcacg gagacatcga gcgcggacac gggctcgtcg
    12841 cagacgatga gcttcggctt gagggcgatg gcgcgggcga tgccgatgcg ctggcgctgg
    12901 ccgccgctga actcaaaggg gaagcggtcg gcggaggcgg agctgaggcc gactttgtcg
    12961 agcagttcga acacccattt ccggcgctct tctttcgagc cgatcttctg cacgatgaag
    13021 ggctcctcca aaatgtcgcg cacggtatgg cgcgggttaa gagattccgc cgggtcctgg
    13081 aaaatcatct gcatgtgccg gcgctgcggg cggatggcgc gcatcgaggc atgggtgatg
    13141 tcctgcccct cgaacacgat tttaccctcc gttggtttga gcaggcgcac gatggccttg
    13201 ccgagcgtgg tcttgccgca gccggattcg ccgacgaggc cgagcgtctc gccctctccg
    13261 agtgtgaaac tgacgccgtc caccgccttg cacgcggcca cagcacggcg cagaacgcct
    13321 ccacgaacgg ggaaatgctg ctggagattt ttgacggtga gaaggctcat gcgacgcaga
    13381 tggggcagtg ctcaacaaaa tgacctgggg cgatctcggt gaccggcggg cgtgtggtga
    13441 gatgttcgct cgtgtgctcg cggccggagc gttcggcgaa gcggcagccg ggcttgaaat
    13501 cggcgatgcc gggcacgttg ccggggatgg ctttgagcag ggttttcggc gtgctgtcga
    13561 ggcgcgggat gctttccagc aggccctggg tataggcgtg gcgcgggttg gcgaagagat
    13621 cgaacacacc ggccttttcc acgatgcgcc cggcatacat gacagcgacc tcatcgcaaa
    13681 cttcagctat gaccccgaga tcgtgcgtga tgaggatgac gctcatgccc atctcttttt
    13741 gcaggtcgga gatcagctcc aaaatctgcg cctgcacggt gacatcgagc gctgtggtgg
    13801 gctcgtcggc gatgagcagc ttcggcttgt tgatgagcgc catggcgatc atcacgcgct
    13861 gcctcatgcc gccgctgagc tggtgcggat attcatggat gcggttctct ggtgaaggga
    13921 tgcgcacgcg ctgaagcatc tcgatgaccc gctgctgcgc ggtggcagga gaaagctccg
    13981 gctggtgcag caaaagcgcc tcggtgagct gacgtccgat cttgtggacc gggttcagcg
    14041 cagtcatcgg ctcctgaaag atcatgccaa tctcactgcc gcgtagtttc cgcagttcct
    14101 catccggcat cgtcacgaga tcgcgccctt cgaagaggat gcgaccgctg agaatccgcc
    14161 cgtggggctg cggcagaagg cggttgatgg aaaaggcggt gacgcttttt ccgcaacccg
    14221 actcgccaac gatgcccaaa gttttcccgg cggtcacgga ccagctcacg ccatccacgg
    14281 cggtcatcaa gccgctgtcc gtctggaacg cggtgacgag attttcgacc tggagaagag
    14341 gctcggacat ggtcgggagt tacttttgcc gccactggtc atagaccttc acgctcttct
    14401 caaaggtctt gccgcttttc atggcttcct gggtggcctt gcgctcgtcc tcatcaatcc
    14461 agaagagcca gttttcctca tggtcacggg cggtgcgggc atcgaaatgg gcggggaatt
    14521 tcatccagcg ccagtgcccg gtgcgatacc acggggactt gaagccggga atgaaaaccg
    14581 ccgcgtcatg gatgcggcgc tgcatcttcc aggccagctc cttcatttcc gcgatgtcct
    14641 cgctcttgtc ataggtgtca atcatcgcgt ccatttcctt gtcgttgaag gaggtgaagt
    14701 tgttgttgtc cacggccagc ttgccttcct gcgtgtaggc gttgacgctg tgccacatct
    14761 cccagaagcg cggatacatc tccacgctga cattgaaggc ggtgaaggcg atgtcgtgct
    14821 gcttctcgtt gaacttcttc cacccggcgg tgttttcagg cgtctcgaca ttgaactcca
    14881 cccccgcttt gcgcgcctcc tccttgatga cgacgagggc ggactccagc actttgtagc
    14941 tgttggtgat ggtgaaggag agccgctggc ctttcgcatc ggcaaaaatg ccgtccttgc
    15001 cgcgctgggt gaagcccgct ttggcaaaac tctccgcagc cttcaccgga tcaaaagtcc
    15061 gcgccttgat gctggtatcc gtgaactcgc caaagccttc ctcggccaga ttgagacgct
    15121 cgtagtcgcc atagaagatc tgctggatga cgacatccca gttgcaggag tgttgcaggc
    15181 cctcacggac atcctggttg cccagcagcg gcttggcggt gttcagccag aagccatagg
    15241 tgggccgtgg cacttgattg taaaaggtgg ccttgtgcag gtagcccttc gccaccatcg
    15301 gatcatcatc gggcacgctc ttgtgccagc gctcgggcag gttcaggcgg taggcgtcga
    15361 gatcgccttt tttgaagact tcgatggcct tgtccgggtc acgaatgacc tccagcacca
    15421 cgcggtcggg attgtattgc tggcggtaaa actttttgtc cttggcccac cagtcttgga
    15481 tgcgggtgaa ggtcacggac tggccttttt tcagtccgcc ctcccgcact tcgtaggccc
    15541 cggtggtggg aatgaagcgc cagttgtaag tttgaagata gtcggggctc atctccttgt
    15601 gaaaggcgat ggcggtgggc tggagcgcgc ccgcatactc gatggcgtcc ggcttcgcgg
    15661 aggcgacgac gacggcgagt gtgagatcgt catacttgat gatctgctcg tacttcttcg
    15721 agaagaagtc atgataccag gtggcctggt tgtgcgggta gcggtagtag aagaagctga
    15781 agaagaagtt gtcggcacgg acaggctcgc cgtcggagaa cttggcgtcg gggtcgagct
    15841 tgaagtaaaa ggtctttttg tcagcgccgc gcgcccactc tttcgcgcag ccgggaatca
    15901 tctccagtgt gttcgggtgg cggcccagca ttttgatcaa aacggagtcc tgcacataat
    15961 cgcggatctc gttgttcgcg tccgggccga tgtggcggag cgtgcgcggg tagtccgtga
    16021 ggaagaagcg gtatgtgccg ccctttttcg cgtccggcga gccaaactcc tcctggtcac
    16081 tgccatcctc ccacttgagg tcggcgggca gatccgtggc ggttttcgtg acgaagaaag
    16141 gctcgccctt ttcattcttc gtctcctcat aaaacttcag cacctcgggg gtgatgtcgg
    16201 tggtgctgcc ggcttctttc ttgccgccgc aggaggcaag aaaggcagcg gccaggagga
    16261 gggcgagttt gagcatggtt ttcatcagcg gtaggtggtg aattttttcg gatcaaaggc
    16321 atcccgcacg gcctcgccga tgaaagcgac gaggaggagc acgaaggtga tggcggagaa
    16381 ggcggaaagg acgatccagg gtgagctgag ctggctcacg ccctcatgca gcatgtctcc
    16441 ccagctcggc gtgggcggcg gcaggccgaa ggcgaggtag tccagcgcat tcagcgcgct
    16501 gatgctgctc atgatggtga agggcaggaa ggtgacgagg gtggaaagcg tgttgggcag
    16561 cacatggcgg aagatgagcc gcatgttgga cgcgccgagc agactggcgg cggccacata
    16621 atcacgggct ttttctttca tcgtcacggt gcgcaggtag gtggcgaggc cggtccagga
    16681 aaagacgatc atgatgacca gcaggatgac gatgcgctgc accaccttca tgtccgccgg
    16741 cagcagcgag atggcgatca tgaccatgta gaggaaggga atcagcgacc aaatctccgt
    16801 gaggcgctgg ccgtagaggt cggtcctgcc gccggtgtaa cccatggtgc agccggcgat
    16861 gatgccgagc agataaaccc ccgccgcata gcagagcgcg aaggtcatgt tcacccggaa
    16921 gccgtaaaaa agccgcgcgg cgatgtcccg cccgattttg tccgtgccga gccagtgcct
    16981 ccgcgccgca tccggcggct gcggatgctg cacgtcatcg cggaagtcgt tttcatacgg
    17041 gtcccaggga atgatgggca tgatgagcca gctctcgctt ttctcagcct tccacttggc
    17101 ctggagttcg cggtagtttg cctcgtaatc gtagtcgagc ccgaaggtgc ggccggattt
    17161 gaagcccgca taggtgggga aatgccactg gccctcatga ctgacaaaga gcgcccgctt
    17221 gttgatccac agctccgcca ccagggccag caggcaaagg atgagcagga tgacaaagct
    17281 ccaccagccc cgcttcagcg cccggaagcg ctgccatttt ttcgcggtga gtgggttcag
    17341 tcgaaacatg gcttctggag ttcactcaaa acggacgcgc gggtcggtca gggccacaaa
    17401 aaagtcggac aggatgtttc caagcagcgt gagcaccccc acgatgacca actgccccat
    17461 gaccacggga taatcccggt ccagcagcga ctggtagccg agcaggccga agccgttgat
    17521 gtcgaaaatg ctctcgatga ggaagctgcc agcgatgaag agcgtcaaat tatgcccgag
    17581 cgaggtggcg atggggatga aggaattccg cagcgcatgg cggcgcaccg cctgcttgaa
    17641 gtcgcagccc tttgccacgg cggtgcggat gtaatccgcg gccagattgt ccagcaggct
    17701 gttttttttc atcatcgtca gaaaggcgaa actgccgacg aggtagcaga tcagcggcag
    17761 cgcggtgtgc tgcaagatgt cccatgcctg gcctaaaagg gagaggtcat caaactcatc
    17821 gctggtgaag ccgccaacgg gaaaccactg ccagcgcgcc gcgcaaaaca cgacgagcaa
    17881 ggcaccgagc gcgaaggatg ggatggcata ccccgcgaag atgaagaccg aggaccaggt
    17941 atccaccggc tgctgatgtt tcaacgcctt gagcacgccg agcggaatgc agatgccata
    18001 gctgagcagg aaggtcatca gcccgaagta agtcgagacc ggcagccgct ccgcgatcag
    18061 atcccacacc ggctccgtgt agcgcgtgga ggtgccgaga tccccctggc agacttttcc
    18121 cagccagtcc gcataagcca gcggccaggg cttgtcgaaa ccgtagaaag ccttgagctg
    18181 ctgcatctgc gactccgaga gcgaggcacc gccgccgccg gggccgctgg agcggccctc
    18241 cgaggaagcc atgcggaact gcatcatcgc cctctccatc ggtccgccgg gcacaaagcg
    18301 cgtgatcgca aacaccagca gcgtcagccc gatcatggtc gggatgatga ggagaaaacg
    18361 ccggatgaag tagtcgcgca tggcgtggtg gagatggcgg ctgaggatta cgcggctggg
    18421 agcgaagtat tcctgccact tgcatccgcg gcttgcgcgc gtctgcggtc cagcagcatt
    18481 ttcacggcgg tcttcatgcg gctgagcaca taataaaccg tctggctgcg caggcgcacc
    18541 aagtccgcgc cgacgatgcg cggcacgaaa tccgccggca cggccagcac ctcctccggc
    18601 gtgctgccat tgagtccgcg gcagaggatg gcggtcatgg ccttggtcag cgtctgcacc
    18661 ttcgggccga gctcgatggc gaagcgcgcc gcgcccgcct cgtccacatg caggaagacg
    18721 cccaccgtgt ccgtgcattc ctcgtccttg cgcacatcct ccagatcgaa gacctgacct
    18781 tcctttctca cacaggaggc ggcggcatcc gagtagtgga tcaaattctc ccgccgctcc
    18841 ggctcgggca gcagttcaaa gaattcgatg atctcttgca gggcggcggg gtaggacatg
    18901 cggcggcagg ctcagatgct cttctcgatc ggggtgcgca ccatgttgcc ccactccgtc
    18961 cagctcccgt catagttgcg caccttctgg tagccgagca ggtatttcaa aacgaaccaa
    19021 gtgtggctgc tgcgctcgcc gatgcggcag taggcgatga tgtcgtcgtc cgtcttcagg
    19081 cccagttcgc cggcatagat ggcctccagc ccggcgcggg atttgaaggt cttgtcctcg
    19141 ttcgccgcgc gcgcccaggg acagctccgc gcgccgggga tgtgaccgcc gcgcagcacg
    19201 ccctcctgcg gatactccgg catgtgcgtc atctcgccgc tgtattcctt cgggctgcgc
    19261 acatccacca gcctgccgcc cgcgcgcatg tgctccagcg tttgctggaa ataggcgcgg
    19321 tggctggcat catcccgccg cgcgggcacg ggatactcag ccggctccgg ctttggcacc
    19381 acgcgggtca gagggcggcc ttcggcttcc catttgtcgc gcccgccatc aagcaccttc
    19441 gccttcgtgt ggccgaaaag ctggaagacc cagaaggcgt agcaggccca ccagttggat
    19501 ttgtcgccgt agaaaatgac cgtcgtgtca ggggtgatgc cattgcgccg gcaaagctcc
    19561 gcgaaagctt ccggccccac ataatcccgt gtcgtctggt cattcaggtc gcgccgccag
    19621 tcaatatgca ccgcgccggg gatgtggccc acgtcataga gcagcaggtc ctcattgctc
    19681 tcgacaatcc ggatgccggg cgtgtcaagg ttggcggcga gccactcggt gtccaccagg
    19741 acatcgggct gggagtattc ggcaaatttg gggttcatgt ttgggagggt aaacgtgcct
    19801 gcgccgtctg gtggattcaa cctcgaaaag cctcttggca ggcttccgcc gcgcgccatt
    19861 catcccgccg atgattcacc aggctttcga gtgctgctcc gtgccgccag agacgatgga
    19921 ccgcttgtgg gcggccgggt ggaggcattt cggcccgcgc tttttccgct acagcctcga
    19981 catgatggat gaccgccccg tcaccatcac acccctgcgt ctggatttgg caaagttcac
    20041 ccccggcaaa ggccagcggc gagtgctgcg caggaatgcc gacacacgct gcgagttcgt
    20101 cccggccagc ctgtccaccg aggcgcgcgc catgtttcac gcgcacaaat cacgcttcaa
    20161 aaccaacatc cctgacgacc tcggtgactt cttttccccc gagcctgcca tcgtcccctg
    20221 cccctgcctc gaatgccggg tttggatcgg tgatgaactg gccgccatca gcttcctgga
    20281 catcggcgcc accgccacct ccgccgtgta tggcatgttt gaccccgccc gctcgcggcg
    20341 cagccttggc atgctcacca tgctccggga gatcgagcac agccgcgccc tggggcgccg
    20401 tttttactat cccggctacg ccactctgga gccggggcct tacgactaca aaaagcagtt
    20461 tgcgggcttg tcccgccttg tctgggagag cggtgagtgg ctgcctttgc caggcgattt
    20521 atccatgcct tcaaatgccg cttgatccgg gctggttcca agctgtcatc atgaaaagca
    20581 cgcctgcttt ctggacgata cttgggcctt cacctcacac aaaacttcca tgcaaccccg
    20641 cctcagcctc attcttcccc ttgcttgtct tctgctggcc ggctgcgccg cgtccggccc
    20701 ctcagacccc gcaggagcgg acattgaaga gtccggcttc aaactgacga aaacccgcgg
    20761 gcgcaacaca ggcaccaacc tccacaaatt gagccgcgcg cgcaggcaga acagtccgcc
    20821 gcgcgagaaa ccttcgcgaa gccgcgtccc gggcatggag gctcctgttg gagatccttc
    20881 ggtcatcgaa acacccgggg caggaccgct tcaggcggat gcccgcaatg caggccctct
    20941 cccggatgcg gcgcctggcg cgcaaccgcc cgcgccgccc cccttcagca aagacccggg
    21001 cccgcacaca agcatcgttc cagcagcgcg cgccggcggc tggtcgcctg tcggcgactt
    21061 tgcgggtgtc cgagtctggt ggcggttcac ccacggaccg gagggtgagg tcgaatgcga
    21121 cttcaaaatg gaaaattcga acccttatcc ggtcgtgatt ttttttgatc ccactttcac
    21181 cgccgccagt ggcggcaagg atcgtggcaa atccgtgcgt gtcacattgc gtgacggcca
    21241 gtcccgaacc acgccgggag gaaggtttga ggctgccgag ggcaaaacgg agggcctgct
    21301 cacccgtccg cccgcagccg gcggagtcag cgacatgact gtcgcgcccg tcttgtgacg
    21361 tgcctgcggt gcctctcaag cctgcggcaa caggtgtgcg gaaaattgaa attcaggatg
    21421 gacttttgcc atcctgccgc tcaaaacgcc gcgccaccag caccagcagc gccgtcacca
    21481 cgcaggacgc ggccagcacg agcacgcctg ccgtgtagcc cgtcgtgcgc tccttcaacc
    21541 agcccatcgc atacggcccg gcaaagctgc cgagattgcc gatgctgttg atgaggccga
    21601 tggccgccgc gttcgccgtg ccagcgagga agctcgtggc ataggcccag aagctgggcg
    21661 tgtagccggt gaggccggtc accgcgatgc acaggcaggc cagcgcgatc cccagatgcc
    21721 cctgccacag caccagcgaa cccagccccg cgcctccaag cagcagcagc ccggccgtgt
    21781 gccagcgcct ctccctcatg cggtcggagg accagcctgc ggcgattttt gcaatcacac
    21841 ccagcgaata gggaagcgac accagcagcg cgacggtgag gtttgagaac ccggacagcg
    21901 actggatcat cgtcggcagc cagtagccga gaccgttcga ggcgatgagg ccgaagaaat
    21961 acgccgccga aaacagcagc acacgcggat catgcagcgc ctgccacacg gtgggctttc
    22021 gcgcctgcat ggcctcgcgg gcgaggcgtt cgcgatcaag ctcagccacc agccaggcct
    22081 tttcatcctc cggcagccac ttcgcatcct gcggcttttc agtcaggaaa aacagcaccg
    22141 agacgcccag caccaccgca gggagcccct ccaggatgaa gagccagcgc cagccctcca
    22201 acccaagcca gtccagcttc aagagcgctc cggaaaccgg tccgccgatg accatcgcca
    22261 gtgagatggc cgacatgaac cacgccaccg cctttccccg gtccaccgcg cggaaccact
    22321 ggccgagata cacgatcatc gccgggaaaa agcccgcctc cgccgcgccg atgagaaagc
    22381 gcagccagta aaactgcgcc gcgttttgga tgaagcccat cagcatcgcc agcacacccc
    22441 aggacaccat gatgcggcac accagccgcc gcacacccca gcgctgggcg aagaccgccc
    22501 ccggcacctc cagaagaaag taacccacaa aaaaaatgcc cgcgcccagc ccataggtct
    22561 ccgccgagaa ccccaggtcc gccgtcatct ccagcgccgc gtaactcaca ttcacccggt
    22621 ccagataggc gatgacatac agcaccacca gatagggcat caaccgccac atcacgcgtg
    22681 agcggactct cgcggctatg cctaaatcag gagacgtcat gcttcgggga gcgtgttatt
    22741 tggccttgag cttctccttc accgccgcgg gcggctccca gccgtaactg cgcttttcat
    22801 tgccgttgta ggcgcaggtg acgacgtaga ggtccatcgt cctcgttttc tcgggcggga
    22861 cattgatctg cacctgcgtg tagccgtcgg cataccaggc ctgatagccg tcggtggcgc
    22921 tttcggtgag cgtgtggccg tttacggcga cattcagcaa ggtgggcgaa cgatagggca
    22981 cgcggaaacg gaagccgatg ccgttttgaa ccgggtcgcc ctgcgtggcc tcacgaggat
    23041 cggaggtgaa ctcgtattcg gggccgagtt tgacgaagcg ctcgatggca tccgcattca
    23101 ctccgggaag ctggcgcagg ttggcgatga atttcgcagg ggcgttgtcg agcgcggcca
    23161 tgcctttcgg cgtcacagcg cagacgtagg tcgcacggcc ctcgtattcg ggatacatca
    23221 tgccatcggc aaaggcgggc tggcggttcc aaagctccac acggctctgg cggcgctgcg
    23281 tcgcggtctg accgtaggcg gtgacaaacc ggcccgcacg ataccgcaca cgcgtcacgg
    23341 gatagccgct gaatttctca tccacccact gcttgtttcc cagcgccagg atgccgcaca
    23401 cgcgagccac gccgctttgc tcccagccga tctcggtggt cgagatcatc gtgtgatact
    23461 tcatgtagcc ataaaacatc gtgcggaagc gatccggcct cggctggttc caaagacggc
    23521 tgcggaagac atcatgatcg ggattgtaaa agcagcgctg tgcatccgcc tcggcccggt
    23581 ccgatccaaa acccgccgcg gcacccgcct gaaccatcgc ctctgtcacg cgcgggtccc
    23641 aggggcgcac gccgcagttc gaatacgagc aggcgctgat ctcaaacatc gtctgcccct
    23701 gccgcatcgt gcggtcgccg agcttgtcgt ctggaaatgc ctgcaaaccg gtgccgtgga
    23761 ggtcgagatg catctccggc tggtattcat ccacgatgcc aagaaacgcc gccagctccg
    23821 gtgtgcgctg cggcacggcc agtttgagct tcggcaggtc ccagtagctg atctgtggat
    23881 cgtagaggtc gatcttctgc ttgttcgtga aacggtccgt ggtgaaaaac gcatacggat
    23941 tggcgatggg cacggccagc accacctgct ttcgtcgtgt ctccttcgcg aggtcggagt
    24001 cgccgagcag ccactcgatc aaatgcagcg tcgtcgtcgt tccagagcgc tccggcccgc
    24061 catgcagcgc ggagaccagc accacctgct tgtcctcgct cgacacggtc gaatccgtga
    24121 tcttcaccag atacaccggc tgcccgtcat gcgattcgcc gcgtttctcg aacgtgaaga
    24181 tccccggatg ctgctccgcc cagtatttca gcgtcgcatc gtattcctcg aagctgatgc
    24241 gatgaatcgg ctccttccaa ggctgcggca attcgtcgag ccggtctttg gggacgtcat
    24301 tggtttgtgc gaggcaaagc aaagcaccga gcgataggag gacgagggcg atgagcttca
    24361 tggtttcttt ttcttccgtg ctggagtggc aggcagtttc ttcagctctt ggacagcggt
    24421 gtcgaagtcc agctccgctt tggagggctt cgccagctcg gcgcggtggt atttgtcata
    24481 ctccagttcc gccttcttca cggcttgttc gtgcgaaatg cggcccgagt gggtgaggat
    24541 gtcacgcccg ccgaggcgta ggaagtcgtc catgcgagcc agccagtcgc tcatatacat
    24601 gggctggcgg ttgagcgcct gcacctccgc cagttccaga aaggcggtca cgatgcggtt
    24661 tagagcgttg agttcctccg gtgtcaggta gttcttggca atgaccgcct ctgacttgcc
    24721 cggattcgcg cccagccagt ttgtcattcc cgcctgcggc ttcgtgccat ccacccgcgc
    24781 atggatgacc tccgcagcgg tctggccgtg ggccgcccag tgcatcttat tctgcacggt
    24841 ggcgaagaac tgccgggagg cctccgtgcc gggatcgtag tcgatgctcg tggcgtagat
    24901 ctccagcacc ttgcgccaga attccttctc cgaggagcgg atgtcccgga tgcgggccag
    24961 cagttcctcg aagtaattgc cgccgccgcg ctgcttgagt cgttcatcgt ccatcgcgaa
    25021 tcccttcacg atgtattcct tcagccgctg cgtggcccag atgcggaatt gtgtgccgcg
    25081 atgcgacttc acccggtagc ccacggagat gatgacatcg aggttgaaat actcgacctg
    25141 gtaggtcttg ccgtcagcgg cagttgttgc aaaatttgca acaactcgat tccgcatcag
    25201 ctcgccctcc tcgaagacgt tgctgatgtg ccgtgaaatg acggacttgt ccctctggaa
    25261 cagatcggcc atctggttca atgacagcca cacgctttca tcctccagcc gcacctgcag
    25321 ccggatgcgg ccgtcgtcgg actggtaaaa gaggaactcg ccgccgcctg gcgggggggc
    25381 tgggatcagg gcgctcatgt caggagggaa gctggccggt tttcagcagg tcgcggaagg
    25441 tgttcagggt ttcggtcttc acgctttcat tcacgagggg gaaggtggcc aggatgtggg
    25501 tgaattcggg ctcggtgagg ccgtagagcc gggccacaag ggcgtccatc tcggcgcgga
    25561 ggcgctggcg ggtggcgggg tccggcacgc cgtggctgcg gtgcgtggcc ttggggccga
    25621 agacttcctt caagaggtcg tcgaacgccg cgctcgtccc caccagccgc gccgcacgct
    25681 ccaccagcgg acggaaggcg gcgtcctggg ccgtcaggcg cgggatgggg agctggtaaa
    25741 cgtagaaaag ggaaacgtgc gatgtgactt tctgccgaat gaggtaatca acaatgaagc
    25801 tgttgaataa cgccacgacg caaagacgaa catgatttga aaagtgtgtt tgattcagca
    25861 ttagccgacc atcctgaacg gagtcgaaat agacttcatc tagccgcact gtatccgggc
    25921 acactacgtt tggcggaaga atggacgcta tcatcgtccg ttcgttcgtg ctggccgcga
    25981 tatcacgaaa cgcaagacga tagctttggt agtcgagctt cacagcgtct ggggaaaccg
    26041 tggctttgat tttgtgctct ttagcgagtg attggatgaa tcggagccgc cccggcagca
    26101 gagcagtgcg cgcctcgctc atatcaaccc aatagcgcgg ctcggaaaat ggatacacga
    26161 actggtgaaa acttttccct tcgacaaggg ggcgcagtcc cttcgctggt tgcggacggt
    26221 aaagcgcggc gtcgtctgtg atgtgaaact cgcggtgaag gaacaactgc cactggcctt
    26281 cgatggtttc gccaagcagc gggaagctaa ggagcttacg agcaagcgct acttcgttcg
    26341 gtgctctgaa ttcggtgagg gagagggagt cgggcgacag tttccgaacg agttcaatct
    26401 ctgtgttgat tgagtcttcg tttggaaacg tcgacaactc ctgaacttca tgccgcatga
    26461 aggctgccgg gaacttcgtt gtcgtgtcgc cggggcggaa ggtgaggacg acgaatttga
    26521 agcggctgtc cacgttctcg aaaatctgct tccggttctc gaagccgaag aggccggtga
    26581 tgcgcgtttt ctcgaagagc atctggcgca gccgcgtggc gccgaggtcg ctgtagatgc
    26641 cgccggggat gacgatgccg cagtagccgc cggtgtggag gaggtggagc gtttgttcga
    26701 ggaagagctt gtagagattg aggtcggtgg tggccttcct gccgttcacg atggcggtct
    26761 ggttctcgta ttgctcggag ttgcggaacc agaggttcac atgcgggaag cgcgagaggt
    26821 aggcgagata tttttcacgc agggcggagt cgccagtgag gacatcggtc ttgcgctcga
    26881 tgaagtcctc cagccgcatc ttgttcttcg acacgtcctc ggccagcggc atgaagaact
    26941 ccttgtcgtc gggcttgaag ttctcccagg gcgggttggt gatgatggcg tcgaagccgc
    27001 cgcgggtgat gaggatgtcg tcgaactcgt aggcccagtg gaagggctgg agggcgcgga
    27061 tgtcttcgag ccgcagcgtg cgtttctcgg gtctgccgtc ctttttcttc gcgctgtccc
    27121 aggtggcctg gtggaactgg atgccgaggc gctggaactc atcgagcagg agcttgtcca
    27181 gcacctcgcg ggcggcggcc ttctgctgct cgatgctgcg gcggagggtg acgagggagt
    27241 cgctgtcctt cgcggcggcg gtgccgatga gttcgggagc ctttttgtag agggcgatga
    27301 ggcggttttt ttcggcgagg agctcggcgt atttcgcagc gcggacggtg gcgaggtggg
    27361 cggcctcgcg ctcggagcgg gtgggggcgg tggtggactc gatggtgagg ccgagttcgt
    27421 ccgcagcggg cgcgtgggtg agggtgaagc gctgctggcc cggtgattga tgatttacaa
    27481 tggatgattg ggcactcccg gaaactccag aaattgttcc agcagattca gggaggcccg
    27541 cagatttgcc tctgaatgaa tctgctggcc tccttgaatc tgcgggtgat gtccggtttg
    27601 gggatgtccc cgtttgatgg tttgagccgt cgaaggcgct ggcatcgacg tggaggaggc
    27661 cgatgaggga attgccggcg aggatgttga actcgatgtt cggcaggggc tcgagctggt
    27721 cgcgcttttc ggcggaggcg acgagggaga ggaagaggcg gagcttggcg atctcggtgg
    27781 cctcctccat gatgtccacg ccgtagaggt tttcggtgac gatcttcttt ttcagccagt
    27841 aggcggcggg ggccttgtgg gcctgctttt cacgggcgat ccagtcgagc acgggcttgt
    27901 gattcaaagc ctcgcagcgg ccgaggaggg cggtgtagag attcagcaat gtcttctgcg
    27961 cggcgacgag aaaggcgccg gagccgcagg cgggatcgag cagggagagg cgcgggagca
    28021 catcatgcag caggcgggcg cagagcgggc cgtcggcatg gaagaggagg tcatcgatgc
    28081 cggtgtagcg gcggggcgcg gggaaggatt tggggttttt caggtgcagc ttggcggcgg
    28141 tgtcgtgccc ggcattggcg cggtcgagca ggagctggtg gatggtctgc tcgcagaggt
    28201 attcggtgat ctcgggccgg gtgtagtagg cgccgaactc cttctggttg atgtatttct
    28261 cgaagatgta gccgagcacg tcgggattga tctcgcggtc gtcgccgccg ggggtgtcat
    28321 tgaggctcca ggagtagcgg cggaagatgg caaagatttc ggtgaaggct tcgtcgggga
    28381 tctttatttt tgcgtaagcg gcggtgagca gggagtcgcc ctcgatgcgc tgctcgatgc
    28441 cgtgggggag gaagaggccg ccattgaggt aagggatgtc ggcgagcggg acggtgccga
    28501 cgggctggcg cttggcgctg ggtttggcga agccctcgaa gaagagatcg cgcagggtgt
    28561 gggagaagaa gcggtttttc cccgcctgct ggctggcggt gaggaggccg ggcaggtagt
    28621 cgcggttttc gccggcgaga aagcctttcg cctggaggaa ccagacgaac atgaggcggt
    28681 tcatgaggac ggaggcatac cagcggcgct cgcggggatc ggggatgcct ttgatgaggt
    28741 cgaggagctt caggtgctcg gtctggaagt cgcggaagaa ctgcttcgtg acggcctcga
    28801 catcgagagc gcgctggacg cgggtggcgg cctcggtgag ggagaactgg ccgttttcat
    28861 ccagctcgga gatgtccaca aagagggcgg cgagctttga gaggaagaga tcgccgggct
    28921 ggccgcgcag gtagtggtgg cggcgcggga tgcgcttttt cccgccctgc tcgcggcgca
    28981 cccagaacca cagggagcgg gtgcgggcgg catcggcgaa gatgaggatg tgctcgtgac
    29041 tggcggcgga gacatgcttc gacacggcga gctgggcggc ggcatccggg agctgcccgt
    29101 cctgcgcgat ggcctcaaac acgcggaagc cggcgagctg ggcgatctcg gtgatgtcga
    29161 aggcggcgga ggactttggc ggagtgaagg aggcgacttt ccgcgaggcg ggcggctccc
    29221 agccgagttc ctcaatgaag agcgggcgga actcgaaggc ctggaggcgc tggcgtgcgc
    29281 gggtggtgtt gagggcggcg ggcatgaggg ggagcagaaa tgacgaatga cgaaatccga
    29341 atgacgaatt gcgggggtca gcgtttggcg cgggatttgg gtttgccgcc cttactgtgg
    29401 gcgaaggtgt ggaagccgat ttcctttttt gacccggcag gaggcggggc catgagctgg
    29461 cggagggcat caaagacgac cttgaactgg ccgtcgtatt tcttttccat ggcctccagc
    29521 ttgcgggcga gctcggcatt cgtggagagc atctggcgga ggcggacgaa ggcgcgcacg
    29581 atagcgatat tcacctgcac ggcacggtca ctgcgcagga cgctggagag catggcgacg
    29641 ccctgctcgg tgaaggcgta gggcgtggca ccgcccaact tgccgcgccc aggtatcaca
    29701 ttttgtgata ccatctcctc cacttcctca tcggttagct caaagagaaa atcctccggg
    29761 aaacgctctg gattgcggcg gacggcctgc ttcaaggccc gtgtctcgac gtcgtagagt
    29821 tcggcaaggt cgaagtcgag catgaccttc tgcgcgcgga tgagcaggat gcggcgctcg
    29881 gcctggaggg cgatggcgtg gtcggcggag gtcatggcag gtggagagag atggcgcggg
    29941 tctggcggca ggtggtgagg gcttgctgcc agtcgagatc gtctgcgaca tcttctggca
    30001 agaccaactg aggtgacgtc agcctgcgaa gcatgcggag atggcgaaac tcgtgctgga
    30061 cgccctcggc ttcattgaag cggcgggctt gttcctgggc gtgggcgaga gtgttcatga
    30121 gcgcgagtgc ggtcagcgtt tggcgcgggg tttgggagtg cgttgagctg gagcaggagg
    30181 caggctttcc ggctcccaag tgtcccggta gaggtggatg tattcggcgg tcacaacagg
    30241 agagatgtaa aagtagtgac ggcaaaggcg gcgaaaaagc gccaacaccc tcgggtgccc
    30301 acaggcatcc agcaatgtgt ccagggcatg ctcaatgcgc gcggcatcgc gacattttga
    30361 acgaatgatg ccatccacct cgggggctaa tgaatcagcc acttgcgctt gaagagtctg
    30421 aagaccttcg acaagacgga tgattccatc gaagttggaa cgcggagcag aaggtgcggt
    30481 cttctttttc atggtggtag atgctgtggc tcactcccga atgccaaggg agcagatgat
    30541 gcgcggctcg cgggcgtggg cctcctccag cgggatgcag aggcggtctt cctcgtgcag
    30601 agtgaggacg agttcggcga gcttgtcatc ggagacgccg gcacgcagct cgcgattgag
    30661 cagatcgcgg gaggcatcgg tgagcggggc ggcgtagagg gcgtcgatgg cgcggtgaag
    30721 gggcttgatg tcgaagaggg tgcctttgac ctcggcggcg tggtctttga ggcgctcgta
    30781 ggtgcggcgg cgggcaccgc cgggcttccc aagctggccg ccagtggtga agtgctcctg
    30841 ctggatgccg gtgacggcct gctggacgag ggcatgatga tttgccagac gcggctgggc
    30901 cggtgtggac ggctcgcact cggcggcacg gaggatttcg tgctggctct cggtgacggt
    30961 gctgccctgc tcgtccaccc aggcgagggc gtcgttgcca tcggcggtgc ggacatagac
    31021 catgacgccg gtggaaggct gcacgccgct ggtgctggca gccgggcgcg gcgggatggc
    31081 ggcgagcgcc ttggtgctga agacgacatt ggcgaggtcg ggaatggttt tgcgcagcgc
    31141 aggatcggca tcgctggcat ttttccaaat ctgccaggcg agcgaggcga gatcgacctc
    31201 gccatcctcg ggatcgtcga ggatgccgga tttttcggtg aagagatcgt ggatgaggcc
    31261 gtcgtgacgc tcgtcctcga agaaggtttc atcggtgccg acgacctcgg cgttttcctg
    31321 caatcgctgg cgcacacgcg cccgcaggcg gatgaggcgc tcgacgccat ccgcagggat
    31381 gaaggagtag cagaggattt cctccgcttt ttgaccgata cgatcgacac gaccggcacg
    31441 ctggatgagg cggatgatgg cccagggcag gtcgaagttc acgatgatgg cggcatcctg
    31501 gaggttctgg ccctcggaga gcacgtcggt ggcgatgagg atgcgcagct catcggctgc
    31561 ggagactttg tcgcgcacct tgttgctgtc cgggctgaag cggcgggtaa agacggaggg
    31621 atcctccgtg ccgccgctga cggcggcgag gcgggtgaca cctttgtcct tcaattgtgc
    31681 ggcgaggtag ttcaccgtgt cggagaactg ggagaagatg agcacctttt gcgcggcgtg
    31741 cgtcttggtg aggagcttgt gcagctcggc gagcttctca tcgcgctccg gctgccagac
    31801 gccggacatc tccagaacgg agaagaggcg ctgggcatcc tgaagcaggt gcttggcgag
    31861 gtcggcgacg aacaggtcgg gccgcagcca gtcgaagttg cgggcgtgat gctgccttag
    31921 cgtctcgtaa gactgcccgg ccacggtttt gaaatcatcg agcgaggcgg cattgatctc
    31981 ggtcagttca ggcggggcat tttcagcatc ctgcccgaag agattgctct cgctgtcgga
    32041 atcttcatcg cgcgtgtcaa agagcgcgga gtcttgcgtg ccgatgggca gcggcaggcc
    32101 gttctcgatg gcgtgaagag cgatgaagtt ccgcaggatg tggcggcgca gggacaggag
    32161 gaaggaatgg ccgctgcttt cgaggcgctt gaagagattc gtgcggcaga agccggtgag
    32221 gcgctgcccg gcacgggaga gattgcgcat gatctccgcc tcgtcctgcg agggtggatg
    32281 gtcgggcgtg gggcgaaggt agtttcccaa accgtaacga ggcaggtgca ggatgcggat
    32341 ggtatcgacc acagtgccgg agtagagctt cgcgaactgg tcgtcggggc ttttttcgcg
    32401 aatgcggaac tcgagcttct tcggctggcg cttcggaaaa tgaaaccgcg tgccgccctc
    32461 cagcagcagg aagcgccgtg ctttcgtgtc ttttgccaca ccacacttgg gacaggcgtc
    32521 ttgcacggtg gagaggatgc catcgcactt tgaacaggga gtccacgcat agttcgcctc
    32581 cacaaagctg cgggtgcggc gcaccatgta gaggcgcatc atctcgcgcc agtcgtcggc
    32641 gtgctcgctt ttctcaaagg cggcgaggca gttcaccggg cactgatggc ggcgcgtgaa
    32701 ctcctccacg cgtccgccgc actcctgccg caggtattcc tccggccgga tgggcacggt
    32761 agcgtccggc tccatgaaga gccggagctg gttggcgagg tcgaggtagg ctttgttgta
    32821 aggcgtggcg gagagcagga tgaccttgct gccgttgcgc gagagataat cgcggatcac
    32881 cgcccagcgc cgcccttcgc ggtttcgcag gttgtggctc tcgtcgatga tgaccacacg
    32941 gtagcggcgc aactctggca ggacattttg cgcctgcgtg atggattcca ctttcgcgat
    33001 gaggcggtaa cgatgcgcat agtcttccca catccctttc agattcttcg ggcagatgat
    33061 aagcgtctcc aacgagcggg gcgggtcttg aaaaacccgc gccagagcag tggccatgag
    33121 tgttttgccg aggcccacga catcgccgag gacgacacca ccgcgttttt ccaatttccc
    33181 cgccgccatg cgcacggcgg ctgcctggaa gtcgagcaga gtgtcccgca tgtcggcggg
    33241 aatctgaaac tcggacaacc ccgcccgcgc ttcctcggcc aagtggtagg ccatcttcac
    33301 ataaatcatg taaggcggca gggcctcaag ccccgcccag ctttcttcga tcacctggat
    33361 cagctccttg gtgatgtcca cgcaccagcg gtcattccag cgatcctcaa accactgcgc
    33421 cagcttttgc gtcgcgtcgt gatcgagcac atcaatgttc agctcgcctt gattcgaaag
    33481 gccggagagc gtgaggttgc tggaaccgag aaagccggtg atgggattgt tcggatcatc
    33541 gcggaagcac aggtagagct tcgcgtgcag cgtgtggcgc aggaaaagct tcacgacaac
    33601 cttgcccgac ttcaattggg cagcgagacg gcgcaacccc gcctcgtccg cgtccgtcgg
    33661 cgctccgaaa gtgagctgat ttcggaaatc ctccgccagc ttgcgcttca tacggatgac
    33721 gagttggttg ctgacctcgt cttcctttgg gagcaagctg agagcatccc gcagctcgtc
    33781 ctgcgcaagc ttttgcatcc cgaccagcag gcggcactgc tgccctgctc cccccgccca
    33841 ttgctcgaac accgagtcga tgcatttcca gccgcgcaaa ttgaagtaac cgacacaaaa
    33901 gtccgagcga tgtcccaagg cgagggtttg ctggagcgcg ggcagcaggt cttgcgcgat
    33961 attatcaaag attcggggca tcaggcatcc tagacagaca gggttggctg tcacaaagca
    34021 tttctacggc acaaactgga gcgcgaataa atccgcctcg atcaggtgaa aacgcagccg
    34081 gacaggcttg cctgcgaggt gggcgaggct gccacctttc catgtcacgg tttgttcggt
    34141 gggaccgcca accatcggtg tgcagtcttc cagcgtgaag ccggggatcg cttttccttc
    34201 ggtgtcctgg atctccacgc gagtttcgat ctggcactcg aggttgagtt gcaacttcga
    34261 tccggcgaaa atcagcggct tggtgatgat ctcgccttgt tttgatgaag cagagacaaa
    34321 accatcgacg cggaaggtga agcgacggat gcggctgcca gggccctggt agtaggcttc
    34381 gctggcatag acggagagtt catccggcct gccgggcagc gcgagcacgc cgatggtcat
    34441 gtagttgctg cggttccagt cgcgatcctt cggcgcggtg atgggaatca gtggttcctc
    34501 ccagcgtttg aagttcacac catcgcggct ggtcatgagc accggctcga cctgcgatcc
    34561 cttgggctga tagcgcgtgg gaaagccgag cagcagatgc ggcgcgcgcg catagggccg
    34621 cacagcattc gtgtagagct gcaccttggg cgcgtccttg ccgtaggcga gatcagcctg
    34681 gttttcccag gtgaggaagt ccttcgaggt cgcggtgcgg atggcgcgca caccggaggg
    34741 cttccagccg cgctcatcgg tatatccgcc ggtgaagtag cgccagtagg cgcggtaggc
    34801 tccgagctcg ttgtcgtaga aagcggtgtt ttgcgaatcg aacgagccgg cagtgatgac
    34861 cggcttttcg cgcatcagcg accaacgcag gccgtcggca gagtgatagg cttgcaacgc
    34921 cgggttcttt ttgcgctcaa cgtcattgcc catggtggca agcgccttgt agcgtgactc
    34981 aggcgggcag tttgggtttg tgtcgagaaa tggcgcgaag gtgccgcaac catcgcccat
    35041 gtgtgtgatg ttgttgtcct tcgatccgcc gaactcaaag atgcctagtt tcggcttcgt
    35101 ccaggtgatg ccatcgcggc tctccgcgta acaggtcacg ccggaatggc cgggccttcc
    35161 tcccggctcg ccatgatgcg atccgcggta gtagcagcgg taaagatcgc cgtcctgaag
    35221 cagcgtgaag taaccggatg tgttcccctc ccatggcgca tcgcacacca gcgccacatc
    35281 ctgcgcctgc ggtttgtgca tctttagctc cacgccggac ttcgaggcga tgaggtgctc
    35341 atccacgaac agctcgcgtc gcgaatcgat gtggatcggc tcagccgcca tgcagaggcc
    35401 gccgagcaat gaaaggatga aaatggcttt catggctgaa gaattttccc cgtcacagag
    35461 ctgaactctc cgctcccagg ctcgaaggga tgccggtcga ggaacccctc cggcaaccgc
    35521 actccaagac caggcgtgtc agcgagccgg atgcggccgt tttcgatcac gggtggttcg
    35581 atccacagat ccgtgagcag cggcgcggtg atgtgcgtgt tgagctccgg gcgcgtggga
    35641 ttgtaggccg ccatctcgca cacagcggtg ttccggcagg caaaggcggc gtgcaggttc
    35701 gcggcgacgc cgggcgtggc actccaggcg tgcgtggcga tgctcacacc ttgcagctcg
    35761 cacaggcggg cgatcttgat gaagttcatg aggccgccca tgaagcttgc gtcgagttga
    35821 gcgagcgcga acggcttgtg ctccagccag tcgcgccact cttcgaggct ggtgagcgtt
    35881 tctccgcccg cgaccggaat tgtggtggag cgccgcagct cgccataggc gcgcatgtcg
    35941 gtgtaaggca gcgcctcttc gaagaagccg atgggatagg cctccagcgc cttcagcacg
    36001 aactgcgcgg tggcgaggtt ccagacatga tcggactcgc caagaaagtc catgtgtgcg
    36061 tcgagattga gctggaagga gtcgccgaac ctttcacgaa gaaactcgac cttgcccacc
    36121 tccacatcgc gcgcctcttg cggcgtgcgc gagccgacaa tcggctgccc aggaccgcga
    36181 taggttcccg cgccgagctt cacagcgtgg aaaccgaggc tcaaatactg cgccaccttg
    36241 ccctccagct cgccacggtc atgcgggctg gttccgcctg tggcatagca tggcagagta
    36301 tcatggcagc gcccgccgag cagctcatac accggcacgc cgagcatctt gcccttcaga
    36361 tcaagcagcg ccgattcaat gccgctgagc acgatggagc caagccccac cctggcccaa
    36421 aactttcctg ccgtgaacat ccgacggctc agcacatcca catcaagggg tgactggccc
    36481 accaggatcg gctcatagaa ccgcacgatt tccggcacca tctccggcac gaaatagccg
    36541 gcataggttt cacccaggcc cgtgagttcc gtgtcggttt cgatcaagac gaaagcggcg
    36601 gagcgtctca cgcgcgactt catgaagatg tcccgctggg gcgcggtgga gagcagggtg
    36661 cgaacgcggg tgattttcat tctaggtcgg cgggtggaat gagattgagc aacggctcgc
    36721 cggcaatgaa gcggagcgca ttctggcgga agatgtccag caatcctgcg ctgaagcgag
    36781 gtgaaaacgt ggtgcctgag atgtgcggcg tgagaatgac atgcgaaaaa tcccacaatg
    36841 gatgctccgc aggcagtgga taggcatagt gcgtgtccag cgccgcgcca ccgaggtggc
    36901 catcactcaa tgtcgtcaac agcgcctgct cctgaatgag cggcccgcgt gcgggattga
    36961 gcacaaaagc gcctttgggc agagacctga gttccgcctc gccaatcagg ccatccgtgg
    37021 cttttgtcag cggcagcgcc agaacaagaa aatcgagacc gctcaaaaac tcgaccttct
    37081 ccccctgcgt gaaataacga tccggcagcg agccatcggg atcaccggtt cctgcaatgc
    37141 aggtcgagcc ggcgcgtgat ttcaccccgg aacgcgagag gacatgcact ctcatgccga
    37201 gagccttcgc caaccgggcc gtttcgcgtc cgatgccgcc gtatccccag atgcccgcgg
    37261 ttttgccgcg aacttccccc gtgaactgag cggaacgctg ccacacatgc gcgtcttgat
    37321 gacggatcag cgtgcgcaga tcacgcacga ggttgatcat catggcgatg ttccattcgg
    37381 cgatcgggca gtcaaaaatg ccgcgcgcat tggtcacgat gatggttttt tcagcgagtc
    37441 ggaacggaaa cagatgcgaa aagcccgccg attcgatctg caaccagcgc aggtttttga
    37501 aggcggccag attgggaggc ggatgtgtgc agaaggccgc cgtgaccctc ccggcatcat
    37561 ccaccggcca tggatctccc tgcttcacat tccgaagcac ccgcatctcc aaaccagcga
    37621 cttccggctc cgatcccggc gggcattcgg tgaagggcaa tgcggtggtt aggcaaagcg
    37681 gcttcatggc atcttcaatt gctcgatgag gaaggcctcc ggctccagtg tgtcaccggg
    37741 cctgtcttga agccggagga tgcacgtcgc acccgcggcg tcgcagcgct ccttcagctt
    37801 cacgccgaaa atggcatgat gaatggcgct gccgggattg ggggcgggca gcggatcgaa
    37861 acgcggatgc tgcaccatga caggcggatc gtccttgctg acatgcgtga tggccgagaa
    37921 ctcgcgccac aatttcaccc actcgggttt tggcttgagc acgtccccgc ctgttttcac
    37981 gccgaccgcg cgggccatca gaggatgttt caagacctcg tcacccgccc attgcctgag
    38041 gatccgcggt tccagggaag gctgcggtga aagcgcgacc acagcgcgca gccgcgtcga
    38101 aggcggaccg agatcatcgt gatacgcgag ccagagcgaa gtcgtggcac cggcggagat
    38161 cccatatgcg gcgaagcgcg cgggatcgag atgccactcc gccgcttttt cacgcagaaa
    38221 ctgcaccgcc ctggcggcat catgcacggg ggccgggagg atggcctgcg tggagtagcg
    38281 gtagttgatc gaggccacgg aaacgccggc caccagcatc gcgtcgagca gcatgggcgg
    38341 caggtcctcc ttgccgccgc ccgcccagcc gccgccgtgg atgaagatga gcagcggcgc
    38401 cggcttcgcg gattttgccc gccagaaatc gagcgtgttg cgctcatgcg tgccgtagct
    38461 gaccttcgcg aaggtggcgg gaggcagggc ggtttgcgcg ggcgctgatc cggcggagca
    38521 actgatcaaa atgatcgcgg tctttgccac ggaatggatt ttcatggcag ccattccggg
    38581 tggttgtgtt gaagcgcgcc gagataacgc tgaaagaccg cgtcgctgac gccggcgtaa
    38641 ggcggcagca ggcgcagcgg gaactcgggg ttgtgaatgc ggcaaaacat cttgtcgaaa
    38701 gctccgtcca tgtgggcgga ggcgcccacg gcctctttga aggcggcaat gaggccgggg
    38761 aaaaccgccg aaattgcttc gagcctggct tcgtcccgcg cgcagccggc ttgaaagaac
    38821 tccgcagcca ttttgaggtt cgtggaggtg aaggaaatga ggaatccgca ctcgcccatc
    38881 agcgccgcct tggcgaaacc cggctccgtg atgaagtgct ggagttgcgg cgcgtccgtg
    38941 aggaggctgc ggagacgagt ctcatccgcc gtgctgtttt tcgtggcgac gaggttttca
    39001 tgctcagcgg caaggcggcc atactcggtg ccggtaagga tacgcccgac gcgcatgagg
    39061 ttgtagtgca gaaaagcgca gtcagaaaag cgtccgcagg tctcacgaaa aaagatctcg
    39121 acctctgcat cgttcaaaac gccccagctt ggcaaactga tctgaaaccg cctcgcgccc
    39181 gtgtcatgcg ctctttcgat gcgctcgatg atggcgggca gcgactggct gatgaggccg
    39241 atcatggcct tcgcgccgcc ttgcgttgtt tcatcaagga aaacccgggt gatctcgtcg
    39301 aactgctttt cggtgacagc gtagccctcg cccgccgtgc cgaagatgta gagatcgcgg
    39361 atgccgctgg cgatctgatg ccgaacggag cggcggaaga tgtcctcctc gaactcgaag
    39421 cgctcattcc aaggcacgca gcaggtggcg aggatgcagc ggggatggcg tttggcgggc
    39481 ttcatcgcag ggtgatggca taaaccgaag ccttgtgcag atgcaggcgg atgtgatgct
    39541 ttccgggcgg caggtcggcg agctttttgt ccttccaggc gaagcggtag cgcatggcat
    39601 ctttcttcaa gggcatgcag ttggccttgt cgaaaccggg caggcggaag ccgtcttcat
    39661 cgaggatttc ggcccacact gcgccttctg atgcgttggc gttcacatag acttcggtgc
    39721 ggcccttcaa atcgagcggt ttgagggtga tctggccttt gtcggcggca gtgacggctg
    39781 cgaagcggtc gagcggcagc gtgacaaggc ccacgccgct cttttgctgg tcgccggtga
    39841 tgttgatgcc gccgccggtg gcaccgctgc tgtaggcacc atagtaaaag cgcatctcgc
    39901 ggccttcgat gacgatgtcc gcattggtgg cgagcgtgcc gctgtcgaac tgctctccag
    39961 ggctgcgttt cagccacaga ttctggcgga aggggcgctc ccactcgatg ccatcgcggc
    40021 tgagcatgag ttcgatgtcc atcacaccgc cgtcggggcg gttgaggatt tggttgaggc
    40081 tgaagtagcg atcatgacgg agaaagacgg gcgaggtgtg aaactcaagc gggccatcgt
    40141 gctcgtcggg cgcgcacacg agctgtggct tcgaccagtg gatgaggtcg gcgctttcga
    40201 cgcgtcccat gccgtgcttc cagccaaggc cgccatcggg accgtggatc cacattttgc
    40261 cgtagatggc ccacacgccg cgtttgggat cgaagagcgg gtcggcggcg tcggacatcg
    40321 tcatgggcag caggagctgg cggcgcacgc gcccgtccgg gagcgaggtc tcgtggtaag
    40381 gactctctcc ggcaaatggc ggctgcatct ttttgccgcc gaaggaggtg gcgtagagcg
    40441 gtgcctcggg atgcttcgtc catcggatgc catccggtga gaaggcgagg tgcagcccgg
    40501 cgatgatctc gcccttcgcg ttcttggccc agtcgtagta gagcatcttg taacgtttcg
    40561 cggcatcgcg ctcgcgcgga tccacgctga cgctgttgca atagcggtcg ccatgcaggc
    40621 cgctgccgat gaggacgatg ttggtcttct tgtcgccgtt gaaatcaaag aggccgaggt
    40681 ccggcttggt gaagctgatg ccatcggcgg actcggcgta acagacgacg cattcgtggg
    40741 tcttgagctg cgcgcgcttg cccgcgtagg cctgatacca catctgatag cgcccggtgg
    40801 ccttgtcgcg atgcacgctc gtccacgcga tggccatctc ccacggcttg tcctcggtga
    40861 tgagcgggtt ctgcggatga cggaccggtt tctcaagcac gcgggtcgtg ccggggcggt
    40921 agaggatgtc atgatcatcc gccatgagga gggtgcgctc ggcggcgctg cctgcggagg
    40981 caaaaatgag cgggatgagc aggttgtatc tcatggttct cgggcgggtt tgattttgcc
    41041 gatgaggaaa tactcgaagt ggctgcctcc ggggccttcg ccaccagcga caaagatcgt
    41101 gtcgccgatg atgcagacgc cggggtcgtt gaagagtccg cccggaggca gcgggctggc
    41161 gatgcgcagc cagcggtcct cgcgcgtgtc atagacgaag gccgcatcgt tccagcgcga
    41221 gcacgcgccg cccacggtga tgagataacg acccgcgaac acatccgcct cccagccgga
    41281 gatgggcacg ggaaaatccg ccagacgggt ccatgtgttc tgcgccggat caaagcgcag
    41341 gcactcggtc agccgcacgc gcccggccgg tgtccaggtc acgccgccga gcatgtaaaa
    41401 ctgcccgtcg agcacggcgg aggcgcacca gccgcgcggc gcgccgggga tgggcgccgc
    41461 ttgccgccag cctttttcag gaagagcgag atcgagcacc tcggtcacac actggatggt
    41521 tttggcgctg tagcccttct cggcaatgtc gtagcgattg cctcctgcga cgatgagcct
    41581 gccgccgacg cgatccgctg ccgtgtgcga acgcggcacg tccagcggcg cgatgctttg
    41641 ccactgcaaa ggcgcccggc gcacatccag acggaagacc tccgcggcgg gaagccccgg
    41701 cctgccttgg gcaaagccgc cgatgatgaa aaacgcgtcg tccgtggcga tggcgcgcgt
    41761 gtattcgcgc cgcgcgggca tgtcgggcag gcgggtccat tcatcggtct gcggatcata
    41821 cacatgcgcc cagcgcgagg tgcggtttcc agcttcctcc gtgccgtcac cggcggggat
    41881 gaaaccgccg gcgtagtaga tcttgccgtc aacgacggcc atgccgccgc cgctcacacc
    41941 gccatgcggc ccaggcgcga tgaagggcat cgcttgcacc gtccattcga tcccgccaaa
    42001 caccggcagc gcctgcaact catccggcat ctcgcgtgag agcgcccttt ccacgcccgc
    42061 gcgctgtgcc gtctgctctt tgagcaagcg tctttcggca aggcgagggt cttcctgggc
    42121 ggcgcagatg cccgtcaaca cgagcatggc gttcaggacg gcggctttct ttttcacggc
    42181 tgcaaaagat aaatggcgct caagcggatg gcagcgcggt ttttgccctc gaagacgcca
    42241 cgcacgcgca cacgatccgg gagcggagcg gcggttttcc actccacggg cacctggaag
    42301 ccgccgcgcg tcacgcgcgc cgcgttttcg ccggaacagc cgggcacggg cttttcgagg
    42361 tgatcgagca gttcgaggcg cagcacggcc tcggcgccca ggccgtccgc attgatgaag
    42421 aaacgcgggg ttttgacatc aagcgatgct gtgacgaatt cggcggtcac ttgcggaagc
    42481 tggtagtcac ccggcccctc tgtgctggtg tccatcatga ggtcggcaaa gcggtcgcgc
    42541 ggcagcaagg cgactccgac accgccgcgc ggctgcggcg ggccgccggt cgggcgtgga
    42601 tcccaagcgc catagtagat gaaagtttgg ccgccgatgt tttcaaaacc ctgtccctgg
    42661 atgaggccgc cctggtccca ttcgccgtca tttccgcgcc cgataaaggt ccattcgtgc
    42721 ttcggctcgc ggaagtggag gccgtcattg ctcatcacga agccgagatc aatgcgcacg
    42781 tccttccact cctccgcgcc atgccagcgg ccatagacgc cgagcagcac gttgcggcgg
    42841 ttccacacgc tgatgccttc gtggttctgc tcgccttcac ggctgcggcc cggaccgaga
    42901 tagtcatgct gcgccgcgcg gacaaaggcg atgctgctcg tgggcgacca gttcacaaaa
    42961 tccgtgctgc gataggtgcg gctcacgcgc ccctgataga ggtgtgtgcc gggcagcgcg
    43021 ttctggccgc tgatgtaaaa ctgcccgtcc cactgataca gcccgccgca aggctcgaag
    43081 tgaaagccgg gaatgaacaa gtcctccttg cgcaactccg ccttgagcgg cttcacctcc
    43141 ttcaccggct tccagcgcag cccgtcggcg ctgacaaagg gcagcagtgt gccgaggcgc
    43201 gtgttgtgcc ggtaatagac atgcgtggtc atcttgtagc ggcgctcggg atcaggatcg
    43261 gcgtcatcgc ggatgacctt gaggttcacg aagccccagg actggccgcc catgtccagc
    43321 aggttgttct ttgtgctgcc ccgatgctcc acaaggccga ggtccggttt cgtccaggtc
    43381 aggccgtcca cgctttccgc ataagccgcc cgccagcgcg cggaggccac cttgttttcc
    43441 gtgtcgtcat cgaaagccac ataccacagg cggtatttgc cgttctcttt gaggatcgag
    43501 ccataaaact gcacgcccat cgcatccggc gcgcccgccg cgccgcgccg cagcaccgga
    43561 ttggcctcat gcctcaccgg ctgccgcatt tcgaggcgca ggttttgcgt gtgagggatc
    43621 gagacatcgt caaaggcgaa aagcgtcacc gcctttgatt catcaaacag ttcctccgcg
    43681 cgcagggtcg aaacaacaag cgccaatcca atgacggcag cttgagggag tgctaaataa
    43741 agcggcatga gagaatataa cgcgcttcat aacgcccggt gccgttttga attactggtt
    43801 caacggcaga accacggacg ggcatgctca ttccttgagg cgcgtgagga tcagacgcgc
    43861 ctttgcaatc ctatggttgc aagcttctga tggtatcact cacacccctg ccagcgcgcg
    43921 agagctgaac ggaggtcgcc ggctgtctgg ctgggactca gctcccagac gagcggcgtc
    43981 tccgcaggga ccagctgcag cagatttgcg aagtccacgc cgccggtgct cagcggcacg
    44041 cggtggtcgc gcgcgggcca gcgaacgtca tggatgtggc agccgatgat gcgcggcgcg
    44101 gtttgagtga ggaattcccc gtgattcaaa agcgccaggt ttgcctgccg ctggacatgg
    44161 ccgaagtcgt gccatgcgcc gatccactgg cagccttcgt gctcgtcgag cagggcgcgc
    44221 atttcttttt gattcgggat ttgctcgtga tggctgcgcg tctcgatgcc gagacgcacc
    44281 ctgtgttttt cacagtctga aagcagcgcg gccagcgcgg cacgcgcgcg ttcgagacgg
    44341 acgggggcgc gttttgcccg cattgccacc agccttagtt tgagggcgca aaaggcgcgc
    44401 gagtagaggc cgccggcatg gacgagcttt tccagcgttg cagtgatggc gggcatgctc
    44461 acccggccca gatgcaccac gacacggtca gcaccaaagc gcgcggccat ctcgatcgtc
    44521 ttgcgcgtta gtgaaacggc gcgctctctc tcccattcct tgtccgaggt gaactcgtac
    44581 acatccggcg cgtccatcat cacctccacg ggcgaggggc agaaattgtg gaggctgctg
    44641 acacgcacga tcccctccgc cacggcgtcg agcaggccgg gtaaaaggga gattttggtt
    44701 ccatgactga cctcgatcca ctcgaagccc agctcgcgcg cctccacggc cagggctttg
    44761 ccttccgtat ggcggtggct gttccagcag gtggacaggg agagttgctg cccggcgggg
    44821 ctgactgcgc gcgtcatcgg ttggcgagtc tttggaagac cttgggagtc tccagcgcag
    44881 ccttcttcgc tttgggcgcg ggtttggggt cgaggttttc caccagatcc tcccggctta
    44941 cgaatgactc aacgccgccc gaaggcaccc cggctttggc gacccagagc agggcattga
    45001 ggatcgtctt gcggaagccg tcgttgcccc agttgcgatg aaaatgcccg ccagtgaaac
    45061 caaagccgcg tccgccgtcg gcgcgctcca cgcaccacat catggcctcc tgcgcacctt
    45121 tctcagcctg gatgtgcggg taggggcctt tgggataaac ataggggcca tcgcgagtcg
    45181 cgtcggatgg ggcggcgacg aggatggggg taaaactcgc ggcaccatcc cgcgccgcag
    45241 tgatgccgtc gccaaaagca gggcggaagc gcatgttgaa ataccactcg tcctcgatct
    45301 ggaaaggctc cacgccgcgc gtgacgggat gctccgtcag cttgtcgaag cgcggctccc
    45361 agatggggtt gcaggaaaag gagttctcgt agtggccgcc gacccactcc agaaactccc
    45421 tgccccccag gtccggcacc acctccacgc cgtagtgcat gacgccgaag cccatgcctt
    45481 ttgccatcag gccgcgcagc gtctccaggc ggccttcctg cacggcaggg tggcctttgc
    45541 cgccgtcggc ataaatgacc accgcgtccg cgtctgcaaa ggtggactcg tcgcgcaccc
    45601 agcccatctc atgccggtcc acgatcaggt tggtgactcc cgccaggcac ttttccaaaa
    45661 gcatgcttcc cgcgcggaac tcatgcatgc cgggcgggtg gga
//
LOCUS       DAOMYJ010000014        45315 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Prosthecobacter sp. isolate AMR_MDS_5629
            NODE_322_length_45315_cov_23.857667, whole genome shotgun sequence.
ACCESSION   DAOMYJ010000014 DAOMYJ010000000
VERSION     DAOMYJ010000014.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867850
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prosthecobacter sp. (wastewater metagenome)
  ORGANISM  Prosthecobacter sp.
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Verrucomicrobiaceae; Prosthecobacter.
REFERENCE   1  (bases 1 to 45315)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 45315)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.30
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:34:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,742
            CDSs (total)                      :: 4,684
            Genes (coding)                    :: 4,676
            CDSs (with protein)               :: 4,676
            Genes (RNA)                       :: 58
            tRNAs                             :: 55
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..45315
                     /organism="Prosthecobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_322_length_45315_cov_23.857667"
                     /isolate="AMR_MDS_5629"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1965333"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..548
                     /locus_tag="PLP58_02620"
     CDS             <1..548
                     /locus_tag="PLP58_02620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ07447.1"
                     /translation="AAPALIAKARQYPGRPPVRYFERDAAQLAGLGEFDAASAILSLQ
                     NMEHLDEVIAAASGVLRLGGRMLWVLNHPAFRIPRQSAWGYEEERKIQYRRVDAYAGT
                     LGIPITMHPGRADSESTVSFHRSLHTLTAAGFRAGLRLSGLEEWYSHKESQPGPRARA
                     ENRARKEFPLFLALLWVKPDE"
     gene            1095..1991
                     /locus_tag="PLP58_02625"
     CDS             1095..1991
                     /locus_tag="PLP58_02625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ07448.1"
                     /translation="MSRRHLTALLCLASLAASSVRAKEPFRPNLGPDLPAITITCGQV
                     RLLLRQASQWTPGRLDFRGAAMTTERSAYGTVFSFPGVGFIGTAHLENEPEELLSLAF
                     FLDDTPLAQPAAELAGGGFRFERRSRIRGFELECCVELRGDRLYETTTVNAAEAVPLR
                     LVYHFMHAWAPSVSAFLAGRDDAPQGGVVVSGALDDGPEGARKFHINQRVDWMAVFEP
                     VSGQYAVSRLLEAPGEGGHVSMLWNVPGSYRKYYLKCFENGTLPAGFQGAWRMVTAFG
                     SASAEAWQPAAMKLAEALRSGD"
     gene            2343..2753
                     /gene="rpsL"
                     /locus_tag="PLP58_02630"
     CDS             2343..2753
                     /gene="rpsL"
                     /locus_tag="PLP58_02630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009963542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HRJ07449.1"
                     /translation="MPTINQLVRHGRKDKTVKSKSPALTKCPQRRGVCLQVMTRTPKK
                     PNSALRKVAKVRLTNGFEVIAYIGGEGHNLQEHSIVLVRGGRVKDLPGVRYHIVRGTL
                     DCLGVDKRRRGRSKYGAKRPKAGAAAAPAGKGKK"
     gene            2787..3260
                     /gene="rpsG"
                     /locus_tag="PLP58_02635"
     CDS             2787..3260
                     /gene="rpsG"
                     /locus_tag="PLP58_02635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007526570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HRJ07450.1"
                     /translation="MARRKRVYDKPLHKDARYDSELVAHLIARVMQCGKKSLAERIVY
                     AAIDQLNEKADSVDPLELFNRAIENAKPKVEVKARRVGGATYQVPLEVAPARSESLAM
                     RWIVGYARGRKGQPMHRALANELKEASQGQGSAVRKRDDVHKQAQANRAFAHFRF"
     gene            3310..5460
                     /gene="fusA"
                     /locus_tag="PLP58_02640"
     CDS             3310..5460
                     /gene="fusA"
                     /locus_tag="PLP58_02640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009963540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HRJ07451.1"
                     /translation="MSNPNSPNREFPLERTRNIGICAHIDAGKTTLTERILFYTGMIH
                     RIGEVHDGAATTDWMEQEKERGITITSAAVTASWKQIKEEGVYKSREGENIRVNIIDT
                     PGHVDFTAEVERSLRVLDGAIFVLCGVAGVQPQSETVYRQAEKYGVPRIAFVNKMDRT
                     GANFENVVKDVRTKLGAPAFPILIPIGAEDSLIGQIDVVNQKAIIYSDDEKFGSKYTI
                     RDLQGDEAAKGKAAYDELLDAVCSADDELGMLFLEEQPIGPVELKAGLRRAVIANKII
                     PMAGGSAFKNKGVQYLIDAVIDYLPGPLDIAPQKGSVVDEPENIIEAIPDDNGKFIAL
                     GFKLWSDKFGRLVFFRVYSGKVSKGDTIYNPRTRKSERVGRLIQIQASVHKDIECCYS
                     GDIAALVGVKDVRTGDTFCDESLEVQLEPPTFPEPVISMAVEPKTKGDQEKMGNALQR
                     LSEEDPTFFVKTDEETGQVIIAGMGELHLEILCDRLKREHKVETNTGKPQIAYRETLT
                     KEADGEGKLVKQSGGRGQYGHVIIKVRPGEKGSGIVVENKVVGGTIPKEFINPAKAGC
                     VEAALNGIIAGYPVIDMHIDLVDGSSHEVDSNENAFKMAAIFAVKDALKKAKCILLEP
                     IMAVECSTPEDYQGDIMGDLNRRRGKIQGMDTRGTTAILRAEVPLAEMFGYSTAIRTL
                     SSGRASYSMQPSHFEQVPQQIVDQIVEQRGGGKN"
     gene            5498..5806
                     /gene="rpsJ"
                     /locus_tag="PLP58_02645"
     CDS             5498..5806
                     /gene="rpsJ"
                     /locus_tag="PLP58_02645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003672692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HRJ07452.1"
                     /translation="MNNQRIRIRLRAYDYRVLDKSTADIVETAKRTGARVAGPIPLPT
                     RIEKFTVNRSPHVDKKSMDQFEIRTHKRMLDIVDPTSRTVDELKKLNLPSGVDITIRI
                     "
     gene            5852..6505
                     /gene="rplC"
                     /locus_tag="PLP58_02650"
     CDS             5852..6505
                     /gene="rplC"
                     /locus_tag="PLP58_02650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012419361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HRJ07453.1"
                     /translation="MALGLIGKKLGMTKVYTDKGVAVSVTVVDVSGNTVIQVKQSAGK
                     DGYTAVQVGYGDQKEFRVTKPAMGHFTKHGVSTPKRHIKEFRLASDDQLPAADAKLDA
                     SLFSAGQWVDVIGTTKGKGFQGVMKRWNFSGQPATHGHMMHRRPGSIGCRLTPGLVWK
                     NQKMPGHDGCERVTTQNLKVIQSRPEDGILLISGAVPGNKGSIVVVRPAKKKPAPAK"
     gene            6524..7135
                     /gene="rplD"
                     /locus_tag="PLP58_02655"
     CDS             6524..7135
                     /gene="rplD"
                     /locus_tag="PLP58_02655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009963537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HRJ07454.1"
                     /translation="MPATVLTVEDARKASLNLTEGHQGKQALHEVVVAYRANRRQGNA
                     HTKNRSEVSGSGKKLWKQKGTGNARMGSKRSPIWSGGGVVFGPRNTTIWAKKTNKNAR
                     RLALRAALTARINDGAVSTVDGFAIADGKTKSFVSAVSGLSDASKVLIIGGAFDELTF
                     RSARNVQSVQLVSAEEVNAEQILNHARIIVTADALPTLARRTA"
     gene            7137..7421
                     /gene="rplW"
                     /locus_tag="PLP58_02660"
     CDS             7137..7421
                     /gene="rplW"
                     /locus_tag="PLP58_02660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012419359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HRJ07455.1"
                     /translation="MKDPHSVIHTIRLSEKATRLGDTNNEYVFLVSPGANKLEIKRAV
                     EAHFGKTVTGVRTANFFGKARRQRRADAGRTNHWKKAIVRLKEGETLDLV"
     gene            7464..8303
                     /gene="rplB"
                     /locus_tag="PLP58_02665"
     CDS             7464..8303
                     /gene="rplB"
                     /locus_tag="PLP58_02665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HRJ07456.1"
                     /translation="MALKTFKPTTPSNRYKEWNSFDEVTKDSPQRSLTVALRKSGGRN
                     NTGRITCRHIGGGHERRYRLIDFKRARRDEAAKVIGIEYDPNRSARIALVEYKDGERS
                     YILAPNGLKDGATVMAGEKAAPEIGNSLPLRKIPLGTSIHNVELVQGRGGVAARAAGQ
                     AAILSNREGDFALVKMPSGEIRKIHAECYATIGQVGNTEHMNVVSGKAGRTRWNGTRP
                     TVRGMCMNPIDHPNGGGEGRSKSGGGRQHLLSPWGHAKGQKTRNPKKATGKLIVQDRR
                     AKK"
     gene            8333..8611
                     /gene="rpsS"
                     /locus_tag="PLP58_02670"
     CDS             8333..8611
                     /gene="rpsS"
                     /locus_tag="PLP58_02670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009963533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HRJ07457.1"
                     /translation="MARSLKKGPYVQQALLEKVDKLNDAGQKRPVKTWSRGSMITPDL
                     VGHTFLVHNGKDFVSVYVTENMVGHRLGEFSPTRVFKSHGAHTVKAAK"
     gene            8632..9417
                     /locus_tag="PLP58_02675"
     CDS             8632..9417
                     /locus_tag="PLP58_02675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ07458.1"
                     /translation="MHRQVQRAILSALPILSAWLALPRAHGQQSFEIQELHTTLQVAA
                     QKIQALEARLAAAESRNEALAQSAAAANQEAGELKERHDRLRGLLEGLGIAALENNQD
                     QLQERLLTALGDLRVSEASRRDLADTLMELAEVSIEFARQSKGVESEASDRLSKTLAR
                     AEEVLVAASSAPLEAEPANLQDARVVSLKPELGIAVLSVGSKDGVKPGMPFEVFREDK
                     PIAKILVTDVRRSVCGAVVQELASKADPVKVGDKGKVDINRSF"
     gene            9421..9816
                     /gene="rplV"
                     /locus_tag="PLP58_02680"
     CDS             9421..9816
                     /gene="rplV"
                     /locus_tag="PLP58_02680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012419355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HRJ07459.1"
                     /translation="MEVRSIYKHARISSQKARQVTRAITGLPVSQALSVLDFTPKKAA
                     FLVGKVLRSAIANAENNHELAADELVVLSAVATPGPTLKRIMPRARGSAAPILKRMSH
                     ITVVIGAREEDEQKDEPKTSKKRKAAAAE"
     gene            9837..10658
                     /gene="rpsC"
                     /locus_tag="PLP58_02685"
     CDS             9837..10658
                     /gene="rpsC"
                     /locus_tag="PLP58_02685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009388013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HRJ07460.1"
                     /translation="MGQKVNPIGFRLAVSKDWRSKWYAPEKEYADALHSDLAIRKYLK
                     ETYFEERAVKDKQAQPMQAAISKIVIERAWNQVRVTLHTARPGLVIGRKGEGIEVMSQ
                     KVSELCGGRQVKIDIFEIKQPELDAQLVAESVAVQLERRISFRRAMKRSVQTAMDMGA
                     DGIRIRVAGRLGGADIARAEQYRQGKVPLQTLRIPIDYGFAEARTVYGIIGVKVWLNR
                     GNAPENSQPRGNDRRRERSDRGGDRGGQGRRGAGDRAPAAQASQAAEAVVEAAAE"
     gene            10714..11133
                     /gene="rplP"
                     /locus_tag="PLP58_02690"
     CDS             10714..11133
                     /gene="rplP"
                     /locus_tag="PLP58_02690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007414620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HRJ07461.1"
                     /translation="MALLPSRVKHRKTQRGSRAGTASRGNKLDFGEFGLQTLDRGWLK
                     NNQIEACRVAINRFLKRKGKVFIRIFPHKPVTARPPETRMGKGKGSPEFWVAVVLPGH
                     MLFEISGVNEATAREACRLAANKLGLRTRFVTRAISH"
     gene            11155..11358
                     /gene="rpmC"
                     /locus_tag="PLP58_02695"
     CDS             11155..11358
                     /gene="rpmC"
                     /locus_tag="PLP58_02695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005229160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HRJ07462.1"
                     /translation="MKTKELRELSIEELGARRRELRQEMLNLRVQQQIGQLENPARLR
                     LLRREVARIETLITQRSTAAKAA"
     gene            11394..11675
                     /gene="rpsQ"
                     /locus_tag="PLP58_02700"
     CDS             11394..11675
                     /gene="rpsQ"
                     /locus_tag="PLP58_02700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009963524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HRJ07463.1"
                     /translation="MSEAATVESQPVRAPVRKERVGVVVSDKMSKTIVVEVERRVPHP
                     RFKKIIRKTSKFYAHDEQGQAKVGDRVLIVETRPLSKLKRWNLLEVLKH"
     gene            11709..12077
                     /gene="rplN"
                     /locus_tag="PLP58_02705"
     CDS             11709..12077
                     /gene="rplN"
                     /locus_tag="PLP58_02705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007073026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HRJ07464.1"
                     /translation="MLQMESSIPVADNTGARVAKMIGVLGKKNTRHAFVGDIITAHVR
                     VSTPTASVKKGEVVRAVVVRTAHPIRRSDGSTLRFDRNAIVIIDKDNNPKGTRIFGPV
                     ARELRDKNFMKIVSLAPEVL"
     gene            12074..12364
                     /locus_tag="PLP58_02710"
     CDS             12074..12364
                     /locus_tag="PLP58_02710"
                     /inference="COORDINATES: protein motif:HMM:TIGR01079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HRJ07465.1"
                     /translation="MSRIKTHVKKGDNVVVISGAHKGAQGVIIEVQPGKGRVLIEGVR
                     MIKKAVRPTQERPQGGIIEKEGPVHISNVKLAEAPKPKAARKSAKKKVAKSS"
     gene            12447..13031
                     /gene="rplE"
                     /locus_tag="PLP58_02715"
     CDS             12447..13031
                     /gene="rplE"
                     /locus_tag="PLP58_02715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007414615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HRJ07466.1"
                     /translation="MEPQLKTLYSEKLRDLLKKQLSLKNIHEVPRLEKIVVNCAVGSQ
                     GERKQAVEDAVNEVQLITGQKPVVTLSKKAIANFKLRAGEAVGVKVTLRGRRMYDFLM
                     RLLKTAIPRIRDFRGVAPKAFDGRGNYTLGITDQSIFPEVELDKVKRTLGFDITFVTS
                     TDNDDHARELLRALGMPFRGKTSAPKAGEQTAAA"
     gene            13061..13450
                     /gene="rpsH"
                     /locus_tag="PLP58_02720"
     CDS             13061..13450
                     /gene="rpsH"
                     /locus_tag="PLP58_02720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007414613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HRJ07467.1"
                     /translation="MTDPISDYLTRIRNAAAAGHQEVFVPFSKMKSELSRVLQEEGYI
                     WSYEVDTSDSHPRLKLKLKYQGKTPVIRHLARVSRPGLRKYVSTDEIPRVLGGLGISI
                     LSTSRGIMTGARARKAKVGGELLAQIW"
     gene            13469..14008
                     /gene="rplF"
                     /locus_tag="PLP58_02725"
     CDS             13469..14008
                     /gene="rplF"
                     /locus_tag="PLP58_02725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009963517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HRJ07468.1"
                     /translation="MSRVGKQPISLPGKVSVKIGDDRSVLVEGPKGKLSWTLPQGVKV
                     ESAGDSVTVTRLSEDRAVRAMHGTTQRLIGNMVEGVSKGFRKDLEIYGVGFRAAVKGS
                     VIDLSLGQSHDRLHPIPAGIKVTVTDNTKIAIEGIDKQAVGQLAADIRAYYPPEPYKA
                     KGVRYAGEQIRRKAGKSVK"
     gene            14032..14391
                     /gene="rplR"
                     /locus_tag="PLP58_02730"
     CDS             14032..14391
                     /gene="rplR"
                     /locus_tag="PLP58_02730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009963516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HRJ07469.1"
                     /translation="MAVINRKLIRDRVHKRIRKNLSGTPGRPRLAVYFSNTNVYAQVI
                     DDVAGRTLVSASTKEKGFDGASKANIATAVKVGQAVAERALAQNITEVVFDRGGFIYH
                     GKVKALADAAREKGLKF"
     gene            14412..14945
                     /gene="rpsE"
                     /locus_tag="PLP58_02735"
     CDS             14412..14945
                     /gene="rpsE"
                     /locus_tag="PLP58_02735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009963515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HRJ07470.1"
                     /translation="MAEPAHSSAFAESSGDSRPDTIEKVVHINRCAKVVKGGRRFSFS
                     ALVVSGDQQGRVGWGFGKANEVADAIKKATEASRKKMNSVHLDNATIPHEVVGEHGGG
                     HIMLKPAAPGTGIIAGGGARAVLEAAGVKDVIGKSLGSSNHANVVKATLAALAALRPK
                     DEILRSRGKTPRERQAL"
     gene            14984..15424
                     /gene="rplO"
                     /locus_tag="PLP58_02740"
     CDS             14984..15424
                     /gene="rplO"
                     /locus_tag="PLP58_02740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018970535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HRJ07471.1"
                     /translation="MRLHDLQPRPGATKRRKRIGCGESSGHGKTSCKGHKGQKARAGK
                     GIRPGFEGGQMPLHRRLPKKGFSNAMFKTAFEVVNVGALNAFEDGATVTEQALREKGL
                     VNRACDAVKILGGGELARSLTVQVDKVSASAREKIEKAGGSIAA"
     gene            15523..17022
                     /gene="secY"
                     /locus_tag="PLP58_02745"
     CDS             15523..17022
                     /gene="secY"
                     /locus_tag="PLP58_02745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012419980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HRJ07472.1"
                     /translation="MISAFANVFKVPELRARILFTLAMIVIVRVGATITLPGVDATVL
                     DEWISTRTADASSGAAQVAALLNVFSGGGLQNCAIFALGIMPYISASIMLQLLTAVVP
                     RLSKLAREDGGRQKITQYTRIATIVLCLFQGYLLARSLQSPGQNIFLGGLQEVIDRLQ
                     RPLVPNFGFFEFVLPTVITITAGTMLMMWLGEMITERGIGNGISLLICVNIVSDLPGA
                     LVQVWQTYVGNVGGDMTKPAMLVLLLGFMVIVIAGVIALTQATRRIVIQYAKRVIGRK
                     VYGGQTQYLPLKINYSGVMPIIFAQAILLFPAQIISQMFPDVAWIQRFSAAIASGWPY
                     YTISAALIFFFSYFWVATMFQPTQISEDLKKSGGYIPGIRPGKPTADFLDFTMSRLTF
                     AGAIFLTLLYILPGIVQAGMGIAPQTAQFFGGTSLLILVGVVLDVMRQVETHLLQSHY
                     DGFLKKGRIRGRFDRIQQTGKVADPATLVRLWIIIAIITLVGVAWFVSK"
     gene            17032..17856
                     /gene="map"
                     /locus_tag="PLP58_02750"
     CDS             17032..17856
                     /gene="map"
                     /locus_tag="PLP58_02750"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009963512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HRJ07473.1"
                     /translation="MGLLSSKIPVRHGAQQQGIRTACQVARDILLKTAAQVRPGVTTG
                     QVDRFAAAEMRARGCKSAFLGYRKFPGHICISVNEEVVHGIGGPRVIQDGDVVKIDVG
                     IEFEGWIGDNALTVPVGNVARETLHLLAATEEALHVAIKHARDGWMLGDLCHSVEDHV
                     TRYGYTVVKEFVGHGVGRKLHEEPQVPNYGVKGARPRLKSGMTLAIEPMINLGTEKTR
                     VLSDKWTVITLDRKPSAHFEHVVLVTEKDPEVLTLRKRMFPKGVEDLTPLPVEALA"
     gene            18189..30920
                     /locus_tag="PLP58_02755"
     CDS             18189..30920
                     /locus_tag="PLP58_02755"
                     /inference="COORDINATES: protein motif:HMM:TIGR02601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter-associated beta strand
                     repeat-containing protein"
                     /protein_id="HRJ07474.1"
                     /translation="MNTRQKACRRLKILRLLGLVSLLPAAASAQSQWLPFFPGGVYSW
                     GDTANWSTGTVPSGAGTSVSFGAIHESQTITLDANRTLGSLAFSNNARAYMLSGSQLI
                     LDNNGLGSTVLSSGIATNLITSAISLADDGGNIFDIDDGRVVLTGVVSGSNELVKNGT
                     GTLILSPSVANTYTGVTTINEGIILAFPQGNNINILGANAAGQHTVINAGGTFATSND
                     LDNNVTGTQSTGGWGTINEAFEINGNGHLGQGALRKMMGREQDTLGGAITMGSAARIQ
                     ADYGTLHHTGTVAINHALTVSGSAGFVSLAGVASGSADIIHYGVSGFRLQANANTYTG
                     TINSNLGEVRADTGDTTTGNNPYDTITALNLRNSALRLIYPNAAGTAPNIASSRFSTT
                     APISMRASLINIENASFNATVTNFFDYALEQTFGVTALESGGNKIYSRSSDAGSVTMI
                     FTDLVRANPGTILELQIDTVQGGAAGNWGASAKHRIINSALEAGPTAVPFVGGWAHYG
                     AEFLKYVPVASAGHGYRQLETSDQSVDTAENTWASGQNIRITSGNRTLPLGITTIQSL
                     NIPSTTARTLSGAAGSTLVVESGGIITGGGVVHTISVPNLTAGNGDSYHLTLIASNSN
                     TIRSSIVDNGGNPVSLVKAGGNTTSFLANNTYTGSTILVEGMFRDIIGARNITALGGG
                     NLNMAGDADSQGTYETDRHFSRALGAGVGEVQLTGGGGFGGGSVGFSAYGAPVNVNFG
                     GAGAEITWGSAHFNPGIFTLNGGNGTHVVTLVNDLDLGGEQRYIRLDGNASGGARQVL
                     GTISGDVSNGGIVRRGGGVLFFDDAKSYTGGTVIQEGELWLRKGTGTAGANVSGNDIM
                     IGAASRLLVDAPSSIGSRQMIVMQNQDDNSASAIAFGPGYGSGSQITFHSRVAANSAT
                     VPQTGAYDITLANQQTGNDRRNRVAVQLSGNHVFTRDVLGQIKTAAPDVEAWFGADTG
                     NGTFTGTTLSSTGRTKTGGNQAFRLGSGGGTLTIANENVLSGAFPLIVGAEDQTARTN
                     IGGVVYLPKAQNYSGTLTTTVGTAVTYGTHIGSGGVLVVGQNGALNTANNTILIRSAE
                     LRLGIDPAESFLGGVDTQYAARNIDVRSGTATLRTIPISGGSNGVLKLNNFTMRMDDA
                     DRSLSLNSIGTHYTATHFDGTAELQNGATARVANFVVGNDSSFQSGVGLLVFNNVISQ
                     TGAGLVHVQKTQGGVLALNADNTYLGETRVNQGRLVIGHEGAAGTPGSTILMNNANDR
                     RADVEFRLDGAGPFTINNVISTTGGNDGSTKVITVGSYNGSSVNKEVRIASLVSNAGG
                     AATTGNTASGLWFDGFGGYRTTITGATTLTRTLTDFRTRGALLTLEGVVGGAGALHKL
                     EQGTLVLSNNNTYTGATNIYNGYVVAAHNNAFGNTTSAVTFNGTAFSQVLASGARTIS
                     RNFTNSATGSTQTIGGLDEGAKTFSGAFTLTRGINVTAVSDGDVTFSGVLSAAGGVNK
                     VGTGLVILSNANTYTGGTTVSNGTLAGVAQAAGSPFGTNGAFTVSDGNLVLQGIAGAT
                     NTATTGVLTVNGGNATIAVNAPAGNTTFTFGSLTRSGNSTLVFKGGNTNIGSPSSEIV
                     SFTAAPSFGPWAVIGGPGTGDNSAHYAGFSGGNVVTATYAGTGDIDGLTGPTDVWDAG
                     AAASTLTGNRSGLAFRTAANVVLGGNTLSLGNAGQAGIILNNGADISGGTIAIGTNAL
                     SIYTDDAAVSTISSVIGNFRDNVNNTLGSGDPVAPSAAALIKFGPGTLELSGTNTFQG
                     NVQVNQGTLSLTAPNVIPTFENLSAVTGSIVTIRPGATVALNGNNQEFGNLTGSVVVS
                     PVLNTAGVLDLGSATLTVGRQGSNALFSGQITGIAGSKLLKIGGGRLTLDNWDPAMPN
                     SLGTLEILQGVVQSRLNDNSWAQAIGFANSIPASTEILLRGGEFEAYVAGDNTSNFQF
                     IPQGHSFTARDGNSIIDTNRWQGSAANKIIGFNNLTVDKNLLTITGGNTIYPRFDGTF
                     TMTANVRLQMDAPGIINGPITGNYTLTKTGASNLEVNSSNDAWNGGAVLHDGVLLFGS
                     RTPEVAERYMAGTNLFSYSATANLGTGDIVVNRGTLIRLNAPSNVLMDDGQRVLLLGD
                     ARNALPRVDIGLDAPLTDYSLRSTGNGALTIGLNDGFYTHAIDQSLLGNGRWQVGAWA
                     TTFYTASSMGSGVDNLFRFGGANGLFQITQGGALSGPSGLWVGADMLPNGFAIANGNG
                     QVRISGDQSYTGNTLVFRNREGGSVQNFLEILGDSASPTFDVYGRLMARGAGRFTNDA
                     GDQVNTVNLHPGSILRLDYVMDVNDTMLVSRLENSNLGLESTENKWGDSAPMLLNGAT
                     LNLVGSSGRVNREVIGVLSVKGGAAVLLERNTTNGQIILETPSITRVGQATFDVRENA
                     DELGRVDLQSQKFYIDNGASMLDAQNMMPVWMINPTRNMFLTYNNDLGVQNRAFSNEF
                     TTGDATAAATFLGGLTSSSIARFAGGQGDPTLAGTVNVHALRIDAVADNETTFTGGQI
                     NIHSGGLIANNNNAAGRVNFNTTNVYFGDGSVAKEGVIYSAGNQITTRFGGVVTAADL
                     TLNGPGQVQLTNVTNAISGNIQLNGGRLFLDGVGTAGTNTTLTLTGNYVQNNDGNQMP
                     ELLLRTLNANGAWTNGIIVAEHTPYVRVLGQSLDGTSLTTVRNQTIPSLMIEGTNTQQ
                     GTTFIFGTTSNTNNANNYNLIVSGATTLGGSAPIGFRIERGGTNIQGGGGFMTLAGAV
                     TGTAPVIKSGDGYLVLQADNTGLSSPVTLNRGEIRGVGNNANNFFGTGDYQLNFGTLR
                     MSTDAARTYFGATGQDLIIGGAVILVNDRNGGAAARTHTFGTNNGTNTIRTENGAHWR
                     MTSDSSDSVVLEGKVIVNDSAMFYTDNAPTFLRDTFEGGGRFTKTGISQVLFDNNVAN
                     SDWKGVLDLQAGFVRVQQPTATLGGVGSSIIINPGAGLSVRSVSNLGTGFGITELRTT
                     SRTTATVIGSMLPADFVSLKNHIHGLGTIGDGVGVLTLSGNQTFTTDPVMASFRDGNW
                     YLGAMHDTGTLSANSVSPWGPGGNKFLLGGGSATLTLNPATAGAQFAGTNQMVIGIPH
                     VVYGYATMVFGANSNNTYDGGTFVSRSRNLDGTYRGTVLSIQAGQVNATTYRTPLGSS
                     LVDVFGEIRIENVNGTARNADTANANTWVFHKGTRLRFDNDNPLNTASTEGRWADNVA
                     IALNTTVLEIYGDGATNAYNTETVGAISVEGGSEVVVRRRGAFLAEIIASGNLTRVGT
                     GTLMLTGMDTNTNTVSGLGSNAATTAMRFKVANGNDLMSNNMVAPWITSRVDGQFLKY
                     TADGFAPITLGGSPANYVSIVNAAATTLTAGGNVPLNDGTEIFSLEGTSNYTLASNLD
                     LYALRLARDINVAANGTANNIFIRSGGLIQVANTPTINANLFFGPDALADGTDVGEAL
                     IWASNNIIQINGKIHASNVIKSGTSELYIRSAQPQFTGDWVVNGGRIRFLTPGAQGGG
                     EIILNGSRMSDRDNVHTITEVLYGFNSGSPDLFDWNGGKITAYDYNRVFANTASDRLQ
                     QLPAMDLRTTNTVAGTGQPGLLLYQVDGNRTTVRTGTVTLYDHYQVHVESGSFGNGST
                     TGVQFGSGSGVGGLNNQGQFDLRKVGDGVLILGDNSLSFTGNRSITIGEGAVRVLHNG
                     AFGGAGNTGNIEQGGALEIAVAGWSPSATLVQQPGSAERWAVNGARSGNVTLPSGVHL
                     QIMHNQTGTQTVTLDGGSIMGYLPRDWDHVAVIHRLGSGVTINLASNSFLGQPYVASN
                     NSLWDTARIYDIGKFNQTNANNPLDQGLRGSYLQIDGVITGPGGLTKLGQDMIVLNGA
                     NTYEGATIVENGILQIGRNNALPVGTDLSTETSSGMFDLNGYNQEVASLSGDHGSVNN
                     GAFDLNTLTLNQSASTTYGGQINGNVSVVKSNTGSLTLTSASGMRGDLVINNGSVILG
                     AAGSVNEARSIQIGAGSVFDVSAVTGGYSYDGRISGGGVGALRADNVSRAQILGSITV
                     TDNVGTIARQGSISPGNSAGHLYVSGDLTLGGGLWGTSTKTERLTLELGAPTSTLAAL
                     GWDGSNVADWLVNVSPDVLNGLAGNLSGHDYVNVGGELTLNEHGGIGVTLINGYQPQY
                     GDVFNLLDWTSVSVGGFDAGPTPRSGGELGYDLNLPDLTAFQLTWHTDLFADYGVIFV
                     VPEPGRMMLLFFGLTGLFLRRRR"
     gene            complement(30973..31953)
                     /locus_tag="PLP58_02760"
     CDS             complement(30973..31953)
                     /locus_tag="PLP58_02760"
                     /inference="COORDINATES: protein motif:HMM:NF014477.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0017013 - protein flavinylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HRJ07475.1"
                     /translation="MPCLPHKRRALWPVIALMAHVLASCSDDPEDGSAVAPLVEFSGS
                     TMGTTWSLCATGAGERHRLLIQTHLDEREAVFSHWREDSALSRFNRQTSTAWFAVPAE
                     LARVVSEARDIARKTGDVLDITCAPLVDAWGFGGTRRAGPPDETELAGAMSHCGWRHL
                     SWRMEPPALRKALPGLRINVASVAEGFVMDELITRLKSEGLRSFMLEIGGEVAGIGTA
                     PDGEPWNIGVRGPGAEYGGIFQTLRLKDQCAATSGTYRHFKAGNPSVNHLLDPRTGRP
                     VDHSLASVTVIHESACQADGFATALMILGPKEGRLAAARLALNAVWIEAE"
     gene            complement(31983..32828)
                     /locus_tag="PLP58_02765"
     CDS             complement(31983..32828)
                     /locus_tag="PLP58_02765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009960824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HRJ07476.1"
                     /translation="MRTALFFLLPLCLDLPAQEVRTEHDVDYLGGGRAEKADLYLPAN
                     PKAGRKHPAVLIIHGGGWSGGDKRAAREINIGTTLAQNGYVGMSINYALADGTHPGPT
                     WPKNLHDCKTAVLWLRANAARLGIDPERIGVIGGSAGGHLAAMVALTGPELDPPGEGS
                     TRVQCAVDLYGPVLWFEQRDIAMFRKSRAEAPELYKQADPRTHIDKDDPPVLILHGTA
                     DKTVPVADSEALAAALKAGGVEHHLEIIPDAPHTFHLQPSQRDLRPLVLGFFDKHLKR
                     QGKGG"
     gene            complement(32864..34303)
                     /locus_tag="PLP58_02770"
     CDS             complement(32864..34303)
                     /locus_tag="PLP58_02770"
                     /inference="COORDINATES: protein motif:HMM:NF013567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HRJ07477.1"
                     /translation="MNTPNRRTFLRQSAFAATALSASRVLGANEKIRLGFIGLGGRGM
                     GSAAWFAKIPGVEIAYLCDADTEVIERAKKKYPQAKTAQDLRKVIEDPETDAVVVSTG
                     NHWHVLASIWACQAGKDVYVEKPVSHNVWEGRKLVEAARKYDRIVQGGTQQRSDPFHL
                     ELKAYLDSGALGKINYVRLNHYGMRQSIGKEEKPITPPATVDYNLWLGPAQDEPIYRP
                     KFHYDWHWVWNTGNGELGNWGPHIIDDLRNIVFRDKISLPKRVLAGGGRYGWDDAGET
                     PNTHFLYIDTGTIPVVMDIHNMPRQKGMKAGDVYLRRRTTAFLVIECEGGYYAGGRGG
                     GSAHDSEGKVIQRFKGDGGGQHAENFIKAMRSRNKTDLNAEIEQIHYSSAWCHLGNLS
                     WRLGKDGDLDMAKARLADFEPWQHVIEDLPAHLAANEVTLKPGDLRVGELLEIDAANE
                     TFTGASATPEALAMLKREYRKGFEVPDKV"
     gene            complement(34388..35065)
                     /locus_tag="PLP58_02775"
     CDS             complement(34388..35065)
                     /locus_tag="PLP58_02775"
                     /inference="COORDINATES: protein motif:HMM:NF016240.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF480 domain-containing protein"
                     /protein_id="HRJ07478.1"
                     /translation="METDTAPPPLTAVEARILGCLIEKELTLPDHYPLTLNALVSACN
                     QSTNREPVMRLDEGEVQRALENMKSRGWVFQVTVVGARVQKYRHNLKGKLPRLEKPAA
                     ALLAVLLLRGSQTVGELRLRSERLQAFPDLESVEAELSGLIAYPEGPVAACLPAGPGR
                     RVAQYAQLLTGEPGGIAPQEEIISPSSAAQPDNAGDQAWKQRMELEIELLKSQINRLK
                     VRLGVED"
     gene            35271..37247
                     /gene="tkt"
                     /locus_tag="PLP58_02780"
     CDS             35271..37247
                     /gene="tkt"
                     /locus_tag="PLP58_02780"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009966105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004802 - transketolase activity [Evidence
                     IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HRJ07479.1"
                     /translation="MNKVLLAQAANEARGLAMDAVHKCSSGHLGLPLGAADIGAVLFG
                     ESLQCDPADPKWLNRDRFILSAGHGSMFIYGWLHLSGYDVSIDDVSKFRVLHSITPGH
                     PEFHETPGVECTTGPLGQGIGNAVGYALSGKMAAAKYNTADHKIIDNHIIALAGDGCL
                     QEGVAREAVAFAAHNGLDNLIVIFDSNDVTLDAMAKVTQSEDAQALYTALGWDAVTID
                     GHDLEAIKAAIDTAKAADNGKPKIIIAKTVIAKGIPEVAGTAKGHGEGGAKFIDAAKK
                     GIGLPEGTHFYVSDEVKGYFADLKAKRAAAHAEWNKSFEAWSAANPEKAAELKAAREG
                     GLKAEDLLKLIPETSGEGKAATRNSGGEILNHIAKAVPHLITGSADLFGSTKNYIKDG
                     GDFSAANPTGRNIWFGIREHAMGAICNGIAYDGLFLASGATFLVFADYCRPSIRLASL
                     AKLPVTYIFTHDSVGVGEDGPTHQPVETVSGLRVIPNLDVIRPADSEEAAAAFAAAFT
                     RTDGPTLLSLSRQDLPHQSHASAAARREGTLKGGYVLVQETAPLEAIVIATGSEVQWA
                     VEGAKGKPGVRVVSLPCFERFDRQSAEYRESVLPASCTKRVAIEAGVSGLWWKYVGTQ
                     GQIIAIDRFGISAPGNLVFKELGITAEAVASALA"
     gene            37360..37515
                     /locus_tag="PLP58_02785"
     CDS             37360..37515
                     /locus_tag="PLP58_02785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ07480.1"
                     /translation="MSTRFFTKSGENTLLKKIAGIFKHNPDIERFDELIVYQRASESV
                     RQKVRRL"
     gene            complement(38045..40480)
                     /locus_tag="PLP58_02790"
     CDS             complement(38045..40480)
                     /locus_tag="PLP58_02790"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HRJ07481.1"
                     /translation="MLRILAVLTALACHGARSQQVPRAIPVEEGERPIPKAIPVGPPP
                     SSPARRKGPDTDLFDYATMIYERGEHTLAAQSFGQYLQTYPSGSDVPMAMFRLGECYL
                     KMNNLKVAETYYREVVDRFPNSDGAPSAAYRLGALTFNARNFAESARHFAFCETKSPL
                     PQVRLAASFNKARSYQQLGDTRRQVTALQAVLAVKGDNPYRENALLSLGTVHLAEDKK
                     ELALPLFLDLVKESKDNPVISEACVKAGVLLAGTGKPDEAVPLFERALLIAETTPANR
                     GIALVGIVQALFAKGDYDGVINNYTRNSTVLPEGETRPRMLLLVGNAYRMKKSYSRAV
                     EVYLMVEQDFPASLQAFEAGYWKLYCFYLLDDKDLGDFATAFIQRHSAAHGDHEFMNL
                     ARLIRADFYFNQADYAKAAVSFGEVLNEALPEKLRAGALFNKGWSYAESGRHQDAAAA
                     FSQFLAEHPAHTLTAKALARRGLAYRDARDVTKAVGDFQRVVKEFTNSDAAEVSYLQL
                     GLIAGEQRDFKAMISAFEMLVKKYPNSPAAAQAWFGIGRAHFDQKEFDKAVPALEKAV
                     EKDRQTYLDRASQMIILALYAQQNVDALAKAVDAYLQASPGAVVPLNTLTWLGLKLYD
                     MREFTRSARYLVLATTPDEPQNTDPRVWNYLGMALLETGDFEKSIAASDHYLAVTPDS
                     AVKARALVTKGRALLALGRLEDAQKIAQDGLAFAKDGKPQAMLLILEADIAMSAGDKY
                     AADGADAEARNKYSAAASKLMIPAQFLVDEELTPEALDKAARALDKAGQPEKAAEFRK
                     LLQEQYPKWQPKR"
     gene            complement(40500..40934)
                     /locus_tag="PLP58_02795"
     CDS             complement(40500..40934)
                     /locus_tag="PLP58_02795"
                     /inference="COORDINATES: protein motif:HMM:NF014525.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HRJ07482.1"
                     /translation="MNFRKQHSIEPTPMQLAPLVDVLLLLVIFFAVTSHYASVEQIMD
                     VSVPAADTGKDDRARNVGEIIVNIKQDGVIIVNGQEVTASQLLRNLANIVAVNKDQAV
                     ILRGDEQVPYQNVVSVLDTCQKAGIWNIAFATRKPDAEAPQN"
     gene            complement(40953..41798)
                     /locus_tag="PLP58_02800"
     CDS             complement(40953..41798)
                     /locus_tag="PLP58_02800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009959814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HRJ07483.1"
                     /translation="MPRKLLSALVLPAILCFWSGAGLQAQNAAPASGETVTLAAPAAA
                     VDEENPFAGALTLDGFIERTGAMAYPLIILSIITAFLIVLFALTVRQGTVVSDAFMNS
                     ADALIRKQDYLGLLAVCNRRNECIARITAKTLDFATRNPTASFEEVKEVTEAEGNRQS
                     SLLLARIAYLGDVGAVAPMLGLLGTTLGMITTFHAISAKDYGGSSQLGLAQGVSEALL
                     CTASGLAIGIPALIFYAIFRGKVNRLISEMEAATTHLMALLAVQYKRAARAVASGRGP
                     DGQPM"
     gene            complement(41871..43634)
                     /gene="argS"
                     /locus_tag="PLP58_02805"
     CDS             complement(41871..43634)
                     /gene="argS"
                     /locus_tag="PLP58_02805"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009961559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004814 - arginine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006420 - arginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HRJ07484.1"
                     /translation="MFRAVLTDRLQAAFTAAGIAAPEGFSPAVVIASDTRYGDYQSNA
                     AMVLAKQLKTNPRALAEQIKAALESVCADLCEKITVDGPGFLNFTLGAPALAQRLAFI
                     IRDAHAGVPQAARPKRVVVDFSAPNIAKPMHVGHIRSTFIGDSLARVARFLGHEVITD
                     NHVGDWGTQFGMIIHGWKTQLDREALKKDAIHELVRVYKTVNAEAKADDAVLEVCKAE
                     LVKLQQGDAENLGIWKECVRLTLEQLEKVYTTLDIRFDHYLGESFYNDALAPLVEDML
                     ASGIATESEGAVCVFSDGSLKPEDDPFLIKEKDSWKAAPCIIRKSDGGFLYATTDLAT
                     IDYRVREWRADEIWYVVGAPQQLHFRQVFAAAKRRGVGTEMTHIAFGSILGPDGKMFK
                     TRSGETVGLLEVIEEAIERAREAVDSRDQGHSAEEKEEIARIIGTAAVKYAELSQNRL
                     TDYKFSWDKMLSLQGNTAPYLLNAYVRTRSIFRKLEGGTVDLAGAKLTITEPAERALA
                     LKLSQFAETVHDILADHRPNLLANYLFELANTYHSFYEACPVLKAEGAARATRLALCE
                     GASRVLKAGLGLLGIQTTERM"
     gene            complement(43718..>45315)
                     /locus_tag="PLP58_02810"
     CDS             complement(43718..>45315)
                     /locus_tag="PLP58_02810"
                     /inference="COORDINATES: protein motif:HMM:NF016693.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tryptophan 7-halogenase"
                     /protein_id="HRJ07485.1"
                     /translation="MRTFGCGVRIALRGAIPSEHDVIIIGGGISGAACGLLLKRALPG
                     LRVLIVERTREFSRKVGESTSEVGACFLTRVLHLGAHLSSRHYQKHGLRMWFCRTPHD
                     RVEDCTEIGPGFQSRLPTFQIDRGILDQHVLDEAERAGCALLRPATLRGIQLADDEKM
                     HTVRVTTAPGVETEHSCRWLVDASGKAALLAKQLGLHRPLGDEHPTSSLWCRFRNVND
                     LDSARSRMAHPDLMKRVKGLRATATNHLTGRGWWVWLIPLSNDDYSAGIVWDRSIFTL
                     PPGPSLTQRLKDHLLTHPIGRLMFAEAEAVEDDTFYYKGLPYHTEQMAGHRWAMVGDA
                     AGFIDPLYSQGLDYCGHTVCAVTDLIRREVQGEDVSASVEYLKGAYKRSYRLWFESLY
                     KGKYEYLGDAELMRIAFIMDLATYFIGPVRLVYDSPDYEWTRLPYDGPAGTFFAKFMS
                     LYNRRLNKLAKTRLAKGTYGAWNNGMDFTLKVSFSPNFSTLRFLRWGIRLWLMAELRT
                     LFGKAPSCAEPAAMPAPAASPAA"
BASE COUNT         8860 a        14866 c        13595 g         7994 t
ORIGIN      
        1 atgccgcccc cgccctcatc gccaaggcgc gccaataccc cggccgcccc ccggtccgct
       61 acttcgagcg ggatgccgcg cagctcgccg gcctgggcga gttcgacgcc gcctccgcca
      121 tcctcagcct gcaaaacatg gagcacctgg acgaggtcat cgccgccgcc tccggggtgc
      181 tgcggctcgg cgggcgcatg ctctgggtgc tgaaccaccc cgccttccgc atcccgcgcc
      241 agagcgcctg gggctacgag gaggagagga agatccaata ccgccgcgtg gacgcctacg
      301 ccggcaccct cggcatcccc atcaccatgc atccgggcag ggcggacagc gagagcaccg
      361 tcagctttca ccgcagcctg cacacgctca ccgccgccgg cttccgcgcc gggctgcgcc
      421 tcagcggact ggaggaatgg tattcgcaca aagaaagcca gcccgggccg agagcgcgcg
      481 ccgagaaccg tgctagaaaa gaattcccgc tcttcctcgc cctgctctgg gtcaagcccg
      541 acgaatgatg aaaaacgcat tttccaccgc cgccgccctc ctgatcaccc tgagcgcctg
      601 ccacacccgg cccgagcccg agcaccggcg ctacttccac accgaagtga agcccgtgct
      661 ggaaattcag tgcctgcgct gccatgccgg ccccactccg cccgcttgtc tctccttgga
      721 gcaccgggac ggcggcgcct acacccgccg cgcggacggg cgcgccttca tcgtgccagg
      781 gcggcccgcg cagagcctgc tcatccagtc catctcccgc ggcggcaccc acccccggct
      841 catgccccgg ctggaggtct ccctcaccga cgaccagatc ggcgccctga ccgagtggat
      901 cgaagacggc gcctggtggc cgcgcggccc cgagggccgc tgcgcgccca gccgaacccc
      961 gagaacccgt gagacgctcc gcaagggcag ccctccagct tcccggacca acgcgcgacg
     1021 gttttttgga ggggtgcggc gtctgcaagc cagcgcgcga cgagttttcg ccggtccctg
     1081 cgtattcctt ccgcatgtcc cgccgccacc tgaccgccct gctttgtctt gcctccctgg
     1141 ccgcctcgtc cgtcagggcc aaggagccct tccggcccaa tctcggcccg gacctgcccg
     1201 ccatcaccat cacctgcggg caggtgcggc tgcttttgcg ccaggccagc cagtggacgc
     1261 cggggcggct ggactttcgc ggcgcggcga tgaccacgga gcgcagcgct tacggcaccg
     1321 ttttttcctt ccccggggtg ggcttcatcg gcaccgcgca cttggagaat gagccggagg
     1381 agctgctctc gctcgccttt ttcctggatg acacgccgct cgcgcagcct gcggcggagc
     1441 tggcgggggg cggcttccgc ttcgagcggc gctcgcgcat ccggggcttc gagctggaat
     1501 gctgcgtcga gctgcgcggc gaccgccttt acgagacgac caccgtcaat gccgccgagg
     1561 cggtgccgct gcggctggtg taccacttca tgcacgcctg ggcgcccagc gtcagcgcct
     1621 tcctggccgg gcgggatgac gcgccgcagg gcggtgtggt ggtctccggc gcgctggacg
     1681 acgggccgga gggcgcgcgc aagttccaca tcaaccaacg ggtggactgg atggccgtct
     1741 ttgagccggt gagcgggcaa tatgccgtct cccgcctgct ggaggccccg ggtgagggag
     1801 gtcatgtgtc catgctctgg aacgtgcccg gcagctaccg caaatactac ctgaagtgct
     1861 tcgaaaacgg caccctgccc gccggtttcc agggcgcctg gcgcatggtg accgcctttg
     1921 gcagcgccag cgcggaggcg tggcagcccg ccgcgatgaa gctcgcggag gcgctgcgga
     1981 gcggggatta aacgcgttcg ttgcttgcgg atatacgggg ctgcgcgggc gggattcgag
     2041 cgcgggcgcg cgcggccatg agaatggggc gcgcgcggca gggggagcgg gcgggtttca
     2101 aaaaaatgcg tccctgggaa aaaagacctt gcggatgttt gcttcgcatc catcatgcgc
     2161 gccctcccag gccccggcat cgctttgatg cttggtgaaa acgccgctca cgcggccttc
     2221 gaccccatcg gagcagtgaa ccggaaaagc actgtgcgac acctttcatc aaggtggtgg
     2281 cactgtctcc ttccgattcc ccgcacggcg cggccagcga accggaaccc ttcaaacaga
     2341 tcatgcccac catcaatcaa ctcgtcagac acggccgcaa ggacaagacc gtgaagtcga
     2401 agtcacccgc gctcaccaag tgtccgcagc gccggggagt ctgccttcag gtcatgacgc
     2461 gcacgccgaa gaagccgaac tccgctcttc gcaaagtcgc caaggtgcgc ctgacgaacg
     2521 ggtttgaagt catcgcctac atcgggggtg agggccataa tctccaggag cactccatcg
     2581 tcctggtgcg cggcggccgc gtgaaggacc tgccgggcgt gcgctaccac atcgtgcgcg
     2641 gcacgctgga ctgcctgggc gtggacaagc gccgccgcgg ccgctccaaa tacggagcca
     2701 agcgccccaa agccggcgcc gccgccgctc ccgccggcaa gggcaagaaa taaccccccc
     2761 atccaacact caaccgacca tccgtcatgg cccgcagaaa acgagtttac gacaagcccc
     2821 tccacaaaga cgcccgctat gacagcgagc tggtggccca cctcatcgcg cgcgtcatgc
     2881 agtgcggcaa gaagtccctg gccgagcgca tcgtctacgc cgccattgac cagctcaatg
     2941 agaaggcgga ctccgtggat ccgctggagc ttttcaaccg cgccatcgag aacgccaagc
     3001 ccaaggtcga ggtcaaggcc cgccgtgtcg gcggcgccac ctaccaggtg ccgctcgaag
     3061 tcgcccccgc ccgctcggag tccctcgcca tgcgctggat cgtcggctac gcccgcggcc
     3121 gcaaaggcca gccgatgcac cgcgcgctgg ccaacgagct caaggaagcc tcccagggcc
     3181 agggcagcgc cgtgcgcaag cgcgacgacg tccacaaaca agcccaggcc aaccgcgcct
     3241 tcgcccactt ccgcttctga tcctcctgtt tccctgacgg caatcccccc agcttccgcc
     3301 aaagtcccca tgtcgaaccc caactccccc aaccgcgaat tcccacttga gcgcacccgg
     3361 aacatcggga tctgcgccca cattgacgcg ggcaagacga ccctgaccga gcgcattctt
     3421 ttctacaccg gcatgatcca caggatcggc gaggtgcatg acggcgcggc caccacggac
     3481 tggatggagc aggagaagga gcgcggcatc accatcacct ccgccgccgt caccgcgagc
     3541 tggaagcaga tcaaggagga gggggtgtac aagtcccgcg agggcgagaa catccgcgtc
     3601 aacatcattg acacccctgg ccatgtggac ttcaccgccg aggtggagcg ctccctgcgc
     3661 gtgctcgacg gcgccatctt cgtgctctgc ggcgtcgccg gcgtgcagcc ccagtccgag
     3721 acggtttacc gccaggctga gaaatacggc gtgccccgca tcgccttcgt caacaagatg
     3781 gaccgcaccg gcgccaactt tgaaaacgtg gtcaaggatg tgcgcaccaa gctcggcgcc
     3841 cccgctttcc cgatcctgat ccccatcggc gccgaggaca gcctcatcgg ccagattgat
     3901 gtggtgaacc agaaggccat catttactcc gacgacgaaa agttcggctc caagtacacc
     3961 atccgcgacc ttcagggcga cgaggcggcc aaaggcaagg ccgcctacga cgagctactg
     4021 gacgctgtgt gcagcgccga cgacgagctt ggcatgctgt tcctggagga gcagcccatc
     4081 ggccccgtcg agcttaaagc cggcctgcgc cgcgccgtca tcgccaacaa aatcatcccg
     4141 atggccggcg gctccgcctt caaaaacaag ggcgtgcagt acctcattga cgccgtgatt
     4201 gactatctgc cgggtcccct cgacatcgcc ccgcagaagg gcagcgtggt tgacgagccg
     4261 gagaacatca tcgaggccat ccctgacgac aacggcaaat tcatcgccct cggcttcaag
     4321 ctgtggtccg acaagttcgg ccgcctggtc ttcttccgcg tgtattccgg caaggtttcg
     4381 aaaggcgaca ccatctacaa cccgcgcacc cgcaagtccg agcgcgtggg ccgcctgatc
     4441 cagattcagg cctccgtcca caaggacatc gaatgctgct attcgggcga catcgccgcc
     4501 ctcgtgggtg tgaaggatgt gcgcacgggc gacaccttct gcgacgagag cctcgaagtg
     4561 cagctcgagc cgccgacctt ccccgagccg gtcatttcca tggccgtcga accaaagacc
     4621 aagggcgacc aggaaaaaat gggcaacgcc ctccagcgcc tctcggagga ggacccgacc
     4681 ttcttcgtca agaccgacga ggagaccggc caggtcatca tcgcgggcat gggcgagctt
     4741 cacttggaaa ttctgtgcga ccgcctcaag cgcgagcaca aggtcgagac caacaccggc
     4801 aagccgcaga tcgcctaccg cgagaccctc accaaggagg ccgatggcga aggcaagctg
     4861 gtgaagcagt ccggcgggcg cggccaatac ggccacgtca tcatcaaggt gcgccccggc
     4921 gagaaaggct ccggcatcgt cgtcgaaaac aaagtcgtcg gcggcaccat tccgaaggag
     4981 ttcatcaacc ccgccaaggc gggctgcgtc gaggccgcgc tcaacggcat cattgccggt
     5041 tatccggtga ttgacatgca cattgacctt gttgacggct ccagccacga ggtggactcc
     5101 aacgaaaacg ccttcaagat ggccgccatt tttgccgtca aagacgcgct gaaaaaggcc
     5161 aaatgcatcc tgctcgagcc gatcatggcc gtcgagtgct ccacgcccga ggattaccag
     5221 ggcgacatca tgggcgacct caaccgccgc cgcggcaaga ttcagggcat ggacacgcgc
     5281 ggcaccaccg ccatcctgcg cgccgaagtg ccgctggccg aaatgttcgg ctattccacc
     5341 gccatccgca ccctttcctc cggccgcgcc tcctactcca tgcagccctc ccacttcgag
     5401 caggtgccgc agcagatcgt tgaccagatc gtcgagcagc gcggcggtgg caaaaactaa
     5461 cccgcccgcc ccaacccctt aacgccggaa cccagtcatg aacaatcagc gaatccgaat
     5521 ccgcctccgc gcctacgact accgcgtgct cgacaaaagc accgccgaca tcgtcgagac
     5581 cgccaaacgc accggcgccc gcgtggccgg ccccatccct cttcccaccc gcatcgagaa
     5641 gttcaccgtg aaccgctccc cgcatgtgga caagaagtcc atggaccagt tcgagattcg
     5701 cacccacaag cgcatgctcg acattgtgga ccccacctcg cgcaccgtgg acgagctgaa
     5761 aaagctcaat ctgccctccg gtgtggacat caccatccgc atctaaccgc gccctcgccc
     5821 tgaaacaacg aacccacagg agacaaacat catggctcta ggactgattg gaaaaaaact
     5881 tggcatgacc aaggtgtaca cagacaaagg cgtggcggtt tccgtcaccg tcgtggatgt
     5941 cagcggcaac accgtcatcc aggtgaagca aagcgccggc aaggacggct acaccgccgt
     6001 gcaggtcggc tacggcgacc agaaggaatt ccgtgtcacc aagcccgcga tggggcattt
     6061 cacaaagcac ggcgtcagca cgcccaagcg ccacatcaag gagttccgcc tcgcctccga
     6121 cgaccagctt cccgccgccg acgccaagct cgacgccagc ctgttcagcg ccggccagtg
     6181 ggtggatgtc attggcacca ccaagggcaa gggtttccag ggtgtcatga agcgctggaa
     6241 cttcagcggc cagcccgcca cccacggtca catgatgcac cgccggcccg gttccatcgg
     6301 ctgccgtctg acccccggcc ttgtctggaa gaatcagaaa atgcccggcc atgacggctg
     6361 cgagcgcgtc acgacccaga atttgaaggt cattcaaagc cgccccgagg atggcatcct
     6421 gctcatcagc ggcgccgtcc ccggcaacaa gggcagcatc gtcgtggtgc gccccgccaa
     6481 gaaaaagccc gcccccgcca agtgaattag gaggaattga accatgccag caaccgttct
     6541 caccgttgaa gacgcccgca aggcgagcct gaacctcacc gagggtcatc agggcaagca
     6601 ggccctgcac gaggtcgtcg tcgcctaccg cgccaatcgc cgccagggca acgcccatac
     6661 caaaaaccgt tccgaagtct ccggcagcgg caagaagctc tggaaacaaa aaggcaccgg
     6721 caacgcgcgc atgggcagca agcgctcccc catctggagc ggcggcggtg tcgtcttcgg
     6781 cccccgcaac accaccatct gggcgaaaaa gaccaacaag aacgcccgca ggctcgcctt
     6841 gcgcgcggct ttgaccgccc gcatcaatga cggcgctgtc tccaccgtgg acggcttcgc
     6901 cattgccgac ggcaaaacca aaagctttgt ctccgccgtc agcggcctga gtgacgcctc
     6961 caaagttctc atcatcggcg gcgctttcga cgagctcacc ttccgctccg cgcgcaatgt
     7021 ccaaagcgtg cagttggtct ccgccgagga ggtcaacgcc gagcaaattc tcaaccacgc
     7081 gcggatcatc gtcaccgctg acgcgctgcc aaccctcgcc cggaggaccg cctgacatga
     7141 aagaccccca ttccgtcatc cacaccatcc gcctgagcga gaaagccacg cggcttggcg
     7201 acacgaacaa cgagtacgtt ttcctcgtca gtccaggcgc caacaagctg gagatcaagc
     7261 gcgccgtcga ggcccacttc ggcaagaccg tcaccggtgt gcgcaccgcc aacttttttg
     7321 gcaaggcccg ccgccagcgc cgcgccgacg cgggacgcac caatcattgg aaaaaagcca
     7381 tcgtccgcct gaaggaaggc gagaccctgg atctcgtctg aacccagcca accaggaact
     7441 tttaaccaga aggaacgcaa gccatggcgc ttaaaacttt caagcccacc acgccctcca
     7501 accgctacaa agagtggaac tcgttcgacg aggtcaccaa ggacagcccc cagcgcagcc
     7561 tgaccgtcgc gctgcgcaaa tccggcggcc gcaacaacac cggacgcatc acctgccggc
     7621 acatcggcgg cggccacgag cggcgctacc gtctcattga cttcaagcgc gcccgccgtg
     7681 acgaggccgc caaagtcatc ggcatcgaat acgatcccaa ccgctcggcg cgcatcgccc
     7741 tggtggaata caaggacggg gagcgctcct acatcctcgc ccccaacggt ctcaaggacg
     7801 gggcgacggt gatggccggc gaaaaagccg cgccggaaat cggcaactcc ctgcccctgc
     7861 ggaagatccc cctcggcacc tcgatccaca atgtcgaact cgtccagggg cgcggcggag
     7921 tcgcggcccg tgccgcgggc caggccgcca ttctctccaa cagggagggt gacttcgccc
     7981 ttgtgaaaat gccctccggt gagattcgca aaatccacgc cgagtgctac gccaccatcg
     8041 gccaggtcgg caacacggag cacatgaacg tcgtgtccgg caaggccggg cgcacccgct
     8101 ggaacggcac ccgcccgacc gtgcgcggca tgtgcatgaa cccgattgac cacccgaacg
     8161 gcggtggcga gggccgctcc aaatccggcg gcggccgcca gcacctgctc agcccctggg
     8221 gacacgccaa gggacagaag acccgcaatc cgaaaaaggc caccggcaag ctgatcgtcc
     8281 aggaccgccg cgccaagaag tgaacccgac ccctgaaacc tgatttatct ccatggcacg
     8341 ctccctcaaa aaaggcccct acgtccagca ggccctcctc gaaaaggtgg acaagctcaa
     8401 cgacgccgga cagaagcgcc cggtcaagac atggtcgcgc ggctccatga tcactcccga
     8461 cctcgtcggc cacaccttcc tggtccacaa cggcaaggat ttcgtgtccg tctatgtcac
     8521 tgaaaacatg gtgggccacc gtcttggcga attctccccc acccgcgtct tcaagagcca
     8581 cggcgcccac accgtcaagg ccgccaagta atccgacttt ccacgaattt catgcaccgc
     8641 caagtccagc gcgccattct gtccgccctg ccgattctct cggcttggct ggcactgccg
     8701 cgcgcgcatg ggcagcagtc tttcgaaatc caggagctcc acaccacgct ccaggtcgcc
     8761 gcccagaaaa tccaggcgct ggaagcgcgc ctcgccgccg ccgaaagccg caatgaagcc
     8821 ctcgcgcaga gcgcggcggc cgccaaccag gaggctggag aactcaaaga gcggcatgac
     8881 cgcctgcgcg gcttgctcga aggcctgggc attgcggctc tggagaacaa ccaggaccag
     8941 ctccaggagc gccttctgac cgcgcttggc gaccttcgag tctccgaagc ttcaaggcgc
     9001 gatctcgcgg acactttgat ggaactggcc gaggtgtcca tcgagttcgc ccgccagtcg
     9061 aaaggcgtcg aatccgaagc ctccgaccgc ctttccaaaa ccctcgcccg cgcggaggaa
     9121 gtgctggtgg cggcatcatc cgcgccgctg gaggctgagc ctgccaacct gcaggacgcc
     9181 cgggttgtca gcctcaaacc cgaactcggc atagccgtcc tgtccgtcgg ctcgaaggat
     9241 ggggtcaagc ccggcatgcc ctttgaagtc tttcgcgaag acaaacccat cgccaaaatt
     9301 ttagtcaccg acgtccgccg ctccgtctgc ggggcggtcg tgcaggaact ggccagcaaa
     9361 gccgatcctg tcaaagtcgg agacaaggga aaagtggaca tcaaccgatc attctaagtt
     9421 atggaagtgc gttcgatcta caaacacgcc agaatctcct cgcagaaggc acgccaagtc
     9481 acgcgggcca tcaccggcct gcctgtctca caggcgctga gtgttctgga cttcaccccc
     9541 aaaaaagccg ccttcctggt tggcaaggtt ctgcgctcgg cgatcgccaa cgctgaaaac
     9601 aaccacgagc tggcagccga cgaacttgtc gtgctcagcg ccgtcgccac ccccggcccc
     9661 acgctcaagc gcatcatgcc ccgcgcccgc ggctcggcgg cccccatcct caagcgcatg
     9721 agccacatca ccgtcgtcat tggagccagg gaggaagatg agcagaagga cgagccgaag
     9781 acctccaaaa aacgcaaggc cgccgccgct gaataatccg cacacaccac agatttatgg
     9841 gacagaaagt caatccgatc ggtttccgcc tcgcagtctc caaagactgg cgctccaaat
     9901 ggtatgctcc ggagaaggag tacgcggacg cgcttcacag cgacctcgcc atccgcaaat
     9961 acctcaaaga gacctacttt gaggagcgtg ctgtcaagga caagcaggcg cagcccatgc
    10021 aggcggccat ttccaaaatc gtcatcgagc gcgcctggaa ccaggtccgc gtcaccctgc
    10081 acaccgcccg ccccggactc gtcatcggac gcaaagggga ggggatcgag gtcatgagcc
    10141 agaaggtctc cgagctctgc ggcggacgcc aggtcaagat tgacatcttc gaaatcaaac
    10201 agcccgaact cgacgcgcaa ctcgtcgccg aaagcgtcgc cgtccagctt gagcgccgca
    10261 tttcatttcg ccgggccatg aagcgctcag tccagaccgc catggacatg ggagccgacg
    10321 gcattcgtat ccgtgtcgcc ggacggcttg gcggcgccga catcgcccgc gccgagcagt
    10381 accgccaggg caaggtcccg cttcaaaccc tgcgcatccc gattgactac ggtttcgccg
    10441 aggcgcgcac tgtctatggc atcatcggag tcaaagtctg gctcaaccgc ggcaacgcgc
    10501 cggaaaacag ccagcctcgc ggcaatgacc gccgccgtga gcgctccgac aggggcggag
    10561 atcgtggagg ccagggacgc cgcggagcag gcgaccgcgc gcccgccgcg caggcttctc
    10621 aggcggccga ggctgttgtc gaggctgccg cagaataagg cgggcattct tccgccgcgt
    10681 ttttcaaacc gaacttttaa ttcctaaatt ctcatggccc ttcttcccag ccgagtaaaa
    10741 catcgcaaaa cccagcgcgg cagccgcgcc ggcacagcct cccgtggcaa caagctggac
    10801 ttcggcgagt ttggtcttca gactctcgac cgcggctggc tcaagaacaa ccagatcgag
    10861 gcctgccgtg tcgccatcaa ccgcttcctc aagcgcaaag gcaaggtttt catccgcatt
    10921 ttcccccaca agccggtgac cgcgcgcccg cctgaaaccc gaatgggcaa gggcaaggga
    10981 tcgccggagt tctgggtggc ggtcgtgctc cccggccaca tgctttttga aatatccggc
    11041 gtcaacgagg ccaccgcgcg cgaggcatgc cgcctggccg ccaacaagct cggtttgcgc
    11101 acccgtttcg tcacccgcgc catctcccac taaactccag accgacgcgc atttatgaag
    11161 accaaagaac tccgcgaact ctccattgag gagctcggcg cccgccgccg cgagctgcgc
    11221 caggaaatgc tgaacctgcg cgtgcagcag cagatcggcc agcttgagaa ccccgcgcgc
    11281 ctgcgcctgc tccgccgcga ggtggcccgc atcgaaaccc ttatcaccca gcgttcgacc
    11341 gccgccaagg ctgcctgaaa ccgcgagtcc caccgattat tctcacaccc atcatgagcg
    11401 aggctgcaac agtagaatcc caacccgtca gagcaccggt gcgcaaggag cgcgttggcg
    11461 tggttgtctc cgacaaaatg tccaagacca tcgtggtcga ggtcgagcgc cgcgtgcccc
    11521 atccgcgctt caaaaagatc atccgcaaga cctccaagtt ttacgcccat gacgagcagg
    11581 ggcaggccaa ggtgggcgac cgcgttctca tcgtggaaac gcgcccgctg agcaagctca
    11641 agcgctggaa cctgctggaa gtcctcaagc actaagccct taacccagaa caaacggagg
    11701 aacccgccat gcttcagatg gaatcatcaa tcccggtggc cgacaacacc ggagcccgag
    11761 tcgccaagat gatcggcgtg ctcggcaaaa agaacacgcg ccacgctttc gtcggcgaca
    11821 tcatcaccgc ccacgtccgc gtctccacgc cgaccgccag cgtcaaaaag ggggaggtcg
    11881 tgcgcgccgt cgtcgtgcgc accgcgcatc cgatccgccg ctcggacggc tccaccctgc
    11941 gcttcgaccg caacgccatc gtcatcattg acaaagacaa caaccccaaa ggaacccgca
    12001 tctttggtcc cgtggcccgc gagctgcgcg acaaaaattt catgaagatc gtctcccttg
    12061 ccccggaagt cctatgagcc gtattaaaac ccacgtcaaa aagggcgaca acgtcgtcgt
    12121 catctccggc gcgcacaaag gcgcgcaggg cgtcatcatc gaagtgcagc ccggcaaggg
    12181 ccgggtgctc atcgaaggcg tgcgcatgat caagaaggcg gtccgcccca ctcaggagcg
    12241 cccgcaggga ggcattattg aaaaagaggg ccccgtccac atttccaacg tcaaactcgc
    12301 cgaggcgccg aaaccgaagg cggccaggaa atcggccaag aagaaagttg ccaaatcctc
    12361 ctgactcccc aatttgcctc cccctccgtt cccgtccggc ttcgccggat ctccggtgga
    12421 caggcggcgc agcacaaaac tgatccatgg aaccccagct caaaaccctc tattcggaaa
    12481 agctccgcga tcttctcaag aagcagcttt ccttgaaaaa catccacgag gtgccgcgcc
    12541 tcgaaaaaat cgtcgtcaac tgcgccgtcg gctcccaggg cgagcgcaag caggccgttg
    12601 aggacgctgt gaatgaagtc cagctcatca caggacaaaa gcccgtcgtc acactcagca
    12661 aaaaggcgat cgcgaatttc aagctccgtg ccggtgaggc tgtcggcgtg aaagtcaccc
    12721 tgcgcggccg gcggatgtat gatttcctca tgcgcctgct gaagaccgcc atcccgcgca
    12781 tccgcgactt ccgcggtgtc gccccgaagg cgttcgacgg ccgcggcaat tacacccttg
    12841 gcatcaccga ccagtccatt ttccccgaag tcgagctcga caaggtcaag cgcacgctcg
    12901 gatttgacat cacctttgtc acttccaccg acaatgacga ccatgcccgc gagcttctgc
    12961 gcgccctcgg catgccattc cgcggcaaga cctccgcgcc caaagccggc gagcagacag
    13021 ccgcagcctg atccgaaaca ccacgacaca gcagcgaatc atgaccgatc ccatttccga
    13081 ctatctcacc cgcatccgca acgcggcggc ggcaggccat caggaggtct ttgtgccttt
    13141 ctccaagatg aagtccgagc tttcccgcgt gcttcaggag gaaggctaca tctggagtta
    13201 cgaggtggac accagcgact cccaccccag gctcaagctc aagctcaagt atcagggcaa
    13261 gacgccggtc atccgccatc tcgcccgcgt cagcaggccc ggactccgca aatatgtctc
    13321 caccgatgaa atcccccgcg ttcttggcgg cctaggcatt tccattcttt ccacctcgcg
    13381 cggcatcatg accggcgccc gcgcgcgcaa agccaaggtc ggcggcgagt tgctcgccca
    13441 aatctggtaa ccccaacgat ccaacatcat gtcacgagtc ggcaaacaac ccatctccct
    13501 gccaggcaag gtcagcgtca aaatcggcga cgaccgcagc gtccttgtgg aaggtcccaa
    13561 gggaaagctt tcctggaccc tgccacaggg cgtcaaagtc gagtccgcag gtgactcggt
    13621 cacggtcacc cgcctgagcg aggaccgcgc cgtgcgcgcc atgcacggca cgacccagcg
    13681 cctcattgga aacatggtcg agggggtcag caaaggattc cgcaaagacc tcgaaatcta
    13741 cggcgtcggc ttccgcgccg ccgtcaaagg cagtgtcatt gacctgagcc tcggccagtc
    13801 ccatgaccgc ctgcacccca tccctgccgg catcaaggtc acggtcacgg acaacaccaa
    13861 gatcgccatc gaaggcatcg acaaacaagc cgtcggccag ctcgccgccg acatccgcgc
    13921 ctattacccg ccggagcctt acaaggccaa aggcgtgcgt tacgccggcg agcagatccg
    13981 ccgcaaggcc ggcaagagcg tcaaataacc ttttttggag aacccactgt catggctgtc
    14041 atcaaccgga aactcattcg cgaccgcgtc cacaaacgca tccgcaagaa cctcagcggc
    14101 acgcccggaa ggccgcgcct cgctgtttat ttctccaaca caaatgtcta tgcgcaggtc
    14161 atagacgacg tcgcgggccg taccctcgtc tcagcctcca ccaaggagaa gggcttcgat
    14221 ggggcgtcaa aagcgaacat cgccaccgct gtcaaagtcg gccaggccgt cgccgagcgc
    14281 gcgctcgccc aaaacatcac cgaagtcgtc ttcgaccgcg gcggtttcat ctatcacggc
    14341 aaggtcaaag ccctcgctga tgccgcccgc gaaaagggcc tgaaattcta atcccgcccc
    14401 tttttcacat tatggcagaa cccgcccaca gctccgcttt cgccgaatcc tccggcgact
    14461 cccgtccgga caccatcgaa aaggtggtcc acatcaaccg ctgcgccaaa gtcgtcaaag
    14521 gcggccgccg cttcagcttt tccgcccttg tcgtctcagg cgaccagcag ggcagggtcg
    14581 gctggggttt tggcaaggcc aacgaagtcg ccgacgccat caagaaggcc acggaagcct
    14641 cgcgcaagaa aatgaactcc gtccacctcg acaacgccac cattccgcac gaggtggttg
    14701 gcgagcacgg cggcgggcac atcatgctga agccagccgc gcccggaacc ggcatcatcg
    14761 ccggaggcgg agcgcgcgcc gtccttgagg ctgccggcgt caaggatgtc atcggcaagt
    14821 ccctcggctc aagcaaccac gccaacgtgg tcaaggccac cctggccgcg ctcgccgccc
    14881 tccggcccaa ggatgagatt cttcgctcgc gcggcaaaac tccgcgagag cgccaggcgc
    14941 tctgagacgc gcccgtcacc caacttcaca gataccgatt tccatgagac tccacgatct
    15001 tcaaccccgc cccggcgcca ccaagcgccg caagcgcatt ggctgcggcg aaagctccgg
    15061 ccacggcaag acgagttgca aaggccataa aggacaaaag gcccgcgccg gcaaaggcat
    15121 ccggcccggc tttgaaggcg gccagatgcc cctccaccgc cgtctgccca agaaaggctt
    15181 cagcaacgcc atgttcaaaa cggcgttcga agtcgtgaat gtgggcgccc tgaatgcctt
    15241 cgaggatgga gccactgtga cggagcaggc gctgcgtgag aaaggcctcg tcaaccgcgc
    15301 gtgcgatgcc gtgaaaatcc tcggcggcgg ggagctggca cgcagcctga ccgttcaggt
    15361 ggacaaagtc agcgcgtccg cccgcgaaaa aatcgaaaaa gccggcggca gcatcgccgc
    15421 ctgagtccac ggaccgaatc ctgtcaagac agcgggccgg ccaggacgcg accttccagg
    15481 ttgccagagg ctcaggcccg ttttctcttt cacacccctc ccatgatctc cgcctttgcc
    15541 aacgtattca aagtccccga attgagggcg cgcattcttt tcactctggc gatgatcgtc
    15601 atcgtccgtg tcggcgccac catcaccctc ccgggtgtgg atgccaccgt gctcgacgag
    15661 tggatcagca cccgcaccgc cgacgccagc tccggcgccg cccaggtggc cgctttgctc
    15721 aatgttttca gcggcggagg actgcaaaac tgcgccatct tcgcgctggg catcatgccc
    15781 tacatcagcg cctccatcat gctccagctt ctgaccgccg tggtgccccg cctcagcaag
    15841 ctggcccgcg aggatggcgg acgccagaag atcacccagt acacccgcat cgccaccatc
    15901 gtgctctgcc tgttccaggg ctatctgctg gcgcgctcgc tgcaaagccc cggccagaac
    15961 attttcctcg gcggcctgca ggaggtcatt gaccgcctgc agcgccccct ggtgcccaac
    16021 ttcggatttt tcgaatttgt cctgcccact gtcatcacca tcacggcggg aaccatgctc
    16081 atgatgtggc tgggcgagat gatcaccgag cgcggcatcg gcaacggcat ttccttgctc
    16141 atttgcgtca acattgtctc cgacctcccc ggcgcgctgg tccaggtctg gcagacctac
    16201 gtcggcaatg tcggcggtga catgaccaag cccgccatgc tcgtgcttct gctgggtttc
    16261 atggtcatcg tcatcgcggg tgtcatcgcc ctcacgcagg ccacgcgcag gattgtcatc
    16321 caatacgcca agcgtgtcat cggccgcaag gtctatggcg gccagaccca gtatctgcct
    16381 ctcaagatca actattccgg cgtcatgccg atcatctttg cccaggccat cctgctcttc
    16441 cccgcgcaga tcatcagcca gatgtttcct gatgtggctt ggatccagcg tttttcggcg
    16501 gcgatcgcct ccggctggcc ctactacacc atctccgccg cgctgatttt cttcttcagc
    16561 tacttttggg tcgccaccat gttccagccc acccagattt ccgaggatct caaaaaaagc
    16621 ggcggctaca ttccgggcat ccggcccggc aagcccaccg ccgatttcct cgatttcacg
    16681 atgagccggc tgacattcgc aggcgccatt ttcctcacac ttctctacat cctcccgggc
    16741 attgtccagg cgggcatggg tatcgccccg cagaccgccc agttcttcgg cggcaccagc
    16801 cttctcattc tggtcggtgt tgtacttgac gtcatgcgcc aggtcgagac acacctgctc
    16861 cagagccatt acgacggctt cctgaagaag ggccgcatcc gcgggcgttt cgaccgcatc
    16921 cagcagaccg gcaaggtggc cgaccccgcc accctcgtca ggctctggat catcatcgcc
    16981 atcatcaccc tcgtcggtgt ggcttggttc gtcagcaagt gaggaccgcg catgggtctg
    17041 ctctccagca aaatcccggt tcgtcacggc gcacagcagc agggtatccg cacggcatgc
    17101 caggtggcgc gggatattct gctcaaaacc gccgcccagg tccgccccgg cgtcaccacg
    17161 ggccaggtgg accgctttgc cgccgctgaa atgcgggcgc gcggctgcaa gagcgccttc
    17221 cttggctacc gcaaatttcc cgggcacatt tgcatctccg tcaatgagga ggttgtgcat
    17281 ggcatcggcg gtccccgtgt gatccaggat ggcgacgtgg tcaaaattga tgtcggcatc
    17341 gagttcgagg gctggatcgg cgacaacgcg ctcaccgtgc ctgtcggcaa tgtcgcccgg
    17401 gagaccctgc atctgcttgc cgccaccgag gaggccctcc acgtcgccat aaagcatgca
    17461 agggacggct ggatgcttgg cgacctctgc cactccgtcg aggatcacgt cacccgttac
    17521 ggttacaccg tcgtcaagga attcgtcggg cacggtgtcg gacgcaagct ccacgaggag
    17581 ccgcaggtgc ccaattacgg ggtcaaaggg gcgcggccaa ggttgaagtc aggcatgacc
    17641 ctggccatcg agccgatgat caatttaggc acggaaaaga cgcgcgtcct ctcggacaag
    17701 tggacggtca tcacccttga ccgcaagcca agcgcccact ttgagcatgt cgtgctggtc
    17761 acggaaaagg atcccgaggt gcttaccctg cgcaaacgca tgttcccgaa aggcgtggag
    17821 gatttgacgc cccttccggt cgaggccctg gcttgaactg cgctcccttc gattttgtcg
    17881 cgacacaacg tccagcagct aaagattttt taccaacgaa ataaagaagt ggctcaatga
    17941 acaacaggag agcagaaagg gagcattcca gagtcctcat gccaataaag aagaaattta
    18001 tcttttcgtc tctcggcctt tgtgtttgaa atcatttaat gacaaaaaaa ttcgaaaaaa
    18061 ttcagcgttc cagccgccac caggacatgc cactgcttcc cgcttgagga gttccgaacg
    18121 aattttcgcc gcatttcaag ccgtccagtt tcccagccat tttcaaaccc aaacgcgcca
    18181 ttccaaccat gaacacacgc cagaaagcct gccgccgtct aaagatttta cgactcttgg
    18241 ggcttgtttc attgcttccc gccgccgcca gcgcgcagtc gcagtggctt cccttcttcc
    18301 ctggaggcgt ttattcatgg ggcgacaccg ccaattggag cacgggaacc gtcccgtcgg
    18361 gcgccggcac ttcggtttca ttcggcgcca ttcatgagag ccagaccatc acgctggacg
    18421 ccaaccgcac gctcggcagc ctcgctttct ccaacaacgc gcgcgcctac atgctctccg
    18481 gcagccagtt gattctcgac aacaacggcc tcggatccac agtgttaagc agcggcatcg
    18541 ccaccaacct catcacctcg gcgatctccc tggccgatga cggcggcaac atcttcgaca
    18601 ttgatgatgg ccgtgtcgtg ctcactggag ttgtcagcgg cagcaatgaa ctcgtcaaaa
    18661 acgggaccgg cacccttatc ttgagtccgt cggtcgccaa cacctatacg ggtgttacca
    18721 ccatcaatga gggcatcatc ctggcctttc cgcagggcaa caacatcaac atccttggcg
    18781 ccaatgcggc cggacagcac acggtcatca acgccggagg cacctttgcc accagcaatg
    18841 acctcgacaa caatgtgacg ggcacccagt caaccggcgg ttgggggacg atcaacgagg
    18901 ctttcgaaat caacggcaac ggtcacttgg gtcagggggc tttgagaaag atgatgggcc
    18961 gcgagcagga caccctgggc ggcgccatca cgatgggaag cgccgctcgc atccaagccg
    19021 attacggcac attgcaccac accggaaccg ttgcgattaa tcacgcgctc actgtcagcg
    19081 ggtcggcggg ttttgtctcg ctggctgggg tggcatcagg tagcgctgac atcattcatt
    19141 atggcgtttc aggatttcgg ttgcaggcta atgccaacac ttacactgga acgatcaatt
    19201 ccaacctcgg ggaagtgcgc gctgacacgg gagataccac aacgggcaac aatccctatg
    19261 acacgatcac agcgttgaac cttcgcaatt cggctttgcg tctgatctac cccaacgctg
    19321 ccggaacagc gcccaacatc gccagcagcc gattctcaac cacggcaccc atttcaatgc
    19381 gcgcgagttt gatcaacatc gaaaacgcgt cctttaacgc cactgtgacc aattttttcg
    19441 actacgcgct tgagcagact ttcggagtca ccgcacttga aagcggcggc aacaaaatat
    19501 actcccggtc atccgacgca ggcagcgtca ccatgatctt caccgacctt gtcagagcaa
    19561 atccagggac tattctggaa ttgcagattg acacagtaca aggcggggct gcggggaatt
    19621 ggggcgcgtc ggccaagcat cgaatcatca actcggcgct tgaggccggc cccactgcgg
    19681 tgcctttcgt cggcggttgg gctcactatg gcgcggaatt cctgaagtat gtcccggtcg
    19741 ccagcgctgg acatggctac cgtcagttgg aaacctccga ccagtccgtt gacaccgctg
    19801 aaaacacctg ggcatcaggt cagaacattc gcatcaccag cggcaaccgc acgcttccgc
    19861 tagggatcac caccattcaa tcactcaaca tcccctccac cactgctcgc acgctctcag
    19921 gcgcggcggg atccacactt gttgtggagt cgggcggcat cattacaggt ggcggtgtgg
    19981 ttcacaccat ctcagtgccg aatcttaccg ctggtaatgg ggacagttac catctcactc
    20041 tcatcgcctc gaactccaac accattcgtt cctccattgt ggacaacggc ggcaatccag
    20101 tctccctcgt caaagcgggc ggcaacacca cctccttcct ggccaacaac acctataccg
    20161 gcagcaccat ccttgtcgaa ggcatgttcc gcgacatcat cggcgcgcgc aacatcaccg
    20221 cgctcggcgg cggcaacctc aacatggcgg gtgacgccga ctcccagggc acctatgaaa
    20281 ccgaccgtca cttctcccgc gctcttggcg cgggcgtcgg cgaggtccag ctcaccggtg
    20341 gcggcggttt tggcggcggc agcgtcggct tttccgctta tggcgcgccg gtcaatgtca
    20401 atttcggcgg agcgggtgcc gaaatcacct ggggcagcgc ccattttaac cccggcattt
    20461 tcaccctcaa cggcggcaac ggcacgcacg tcgtcactct ggtcaatgat ctcgatctcg
    20521 gaggtgagca gcgctacatc cgcctggatg gcaatgcttc agggggggcg cgccaggttc
    20581 tgggcaccat cagcggcgat gtcagcaatg gtggcatcgt taggcgcggc ggtggcgtgc
    20641 tcttctttga tgacgccaag agctacaccg gtggcacagt catccaggag ggcgaactct
    20701 ggctgcgcaa gggcactggc acggctggcg ccaatgtcag tggcaatgac atcatgatcg
    20761 gtgccgcgtc ccgcttgttg gttgatgccc cttccagcat tggctcgcgc cagatgatcg
    20821 tcatgcaaaa tcaggacgac aacagcgcct ccgccatcgc cttcggcccc ggttacggca
    20881 gcggctcgca gatcactttc cacagccggg tggccgccaa cagcgccact gtgccgcaaa
    20941 cgggtgctta tgacatcaca ctggcaaatc agcagactgg caatgaccgc cgcaaccggg
    21001 tggctgtgca actcagcggc aaccatgtct tcaccagaga cgtgctgggg cagatcaaaa
    21061 cagcggctcc tgatgtcgag gcttggttcg gtgcggatac cggcaacggc acttttacag
    21121 gcacgacgtt gtcgtccaca gggcgcacca aaacaggggg caaccaggca ttccggctcg
    21181 gttccggcgg cggcacgctc accatcgcca atgaaaatgt cctgagtggc gcgttccctc
    21241 tcattgtcgg tgcggaggat cagacggcgc gcaccaacat tggcggtgtc gtttatctac
    21301 ccaaagccca gaactattca ggcacgctca ccaccacggt gggtacggcg gtgacatacg
    21361 gcacccatat cggttccggt ggagttctgg tagtgggtca gaatggtgct ctcaacacag
    21421 ccaacaacac gattttgatc cgctccgccg agttgcgcct cggcattgat ccggcggagt
    21481 ccttccttgg cggcgtggac acacagtacg ccgcgcgcaa cattgatgtg cgcagcggca
    21541 cggccaccct gcgaaccatt cctatctccg gtggcagcaa tggcgtgctt aagctcaata
    21601 atttcaccat gcgcatggat gacgctgatc gcagcttgag cctgaactcc atcggcacgc
    21661 attacacggc cacacatttt gatggcacag cggaattgca gaacggggcc acggctcgtg
    21721 tcgccaactt tgtggtcggc aatgacagca gcttccagtc tggcgtcgga ctcctcgtct
    21781 ttaataatgt gatcagccag acgggggccg gtcttgtgca tgtgcagaag acccagggcg
    21841 gtgtgctcgc gctgaatgcc gacaatacct accttggcga gacacgcgtc aaccaggggc
    21901 gtctggtaat cggtcacgag ggcgcggcag gcacaccagg ctcgaccatt ttgatgaaca
    21961 acgccaatga ccgacgcgcg gatgtcgaat tccgtcttga tggtgccggt cccttcacga
    22021 tcaacaacgt catcagcacc acggggggca atgacggctc cactaaagtc atcacagtgg
    22081 gatcttacaa cggaagcagc gtgaacaagg aagtgcgcat tgccagcctc gtcagcaatg
    22141 cgggcggagc cgcaacgact ggcaacactg cctccggatt gtggtttgac ggttttggtg
    22201 gttaccgcac caccatcacg ggtgccacca ctctcacccg cacgttaacg gacttccgca
    22261 cgcgtggagc gctcctgacc cttgaaggcg tcgtgggtgg agccggcgct ctgcacaaac
    22321 ttgagcaggg cacgctcgtc ctctccaata acaacaccta caccggcgcg accaacatct
    22381 acaacggtta cgtcgtcgcc gcccacaaca acgcttttgg caacaccacc agcgcggtca
    22441 cgttcaatgg caccgcattt tcacaagtcc tcgcttcagg tgcccgcacc atcagccgca
    22501 acttcaccaa cagcgccacc ggcagcaccc agaccatcgg cggcctggac gagggcgcca
    22561 agacgttcag cggtgccttc accctgaccc ggggaattaa cgtgacagcg gtgagcgacg
    22621 gtgatgtcac cttcagcgga gtcctgagcg ccgccggcgg cgtcaacaag gttggaacgg
    22681 gtcttgttat tctttccaat gccaacacct acacgggcgg gaccacggtg agcaatggca
    22741 cactggcagg cgttgcccag gctgccggca gtcctttcgg gaccaacggc gctttcaccg
    22801 tgtcggatgg aaatctggtt ctccagggaa tcgcgggagc gactaacacc gccacgactg
    22861 gagtcctgac agtgaacggg ggcaatgcca cgatcgccgt gaacgctcct gccggtaaca
    22921 ccaccttcac ctttggttct ctgacccgct ccggcaacag caccctggta ttcaaaggcg
    22981 gcaacacgaa catcggctcc ccgagcagtg agatcgtttc gttcaccgcc gcgccgagct
    23041 tcggcccgtg ggcagtgatt ggcggtccgg gaacaggtga caattcagcg cactatgccg
    23101 gcttctcagg cgggaatgtc gtgacagcca cttatgccgg caccggagac attgacggtc
    23161 tcactggtcc cactgatgtg tgggacgcag gggctgcagc cagtactttg acgggaaatc
    23221 gcagcggcct tgccttccgc accgccgcca atgtcgtcct cggcggcaac accctgagcc
    23281 ttggcaacgc cgggcaggcg ggcatcatcc tcaacaacgg cgcggacatc tccggcggca
    23341 cgatcgccat aggcacgaat gctctgagca tttatacgga tgacgccgct gtctccacca
    23401 tcagttctgt gattggcaat ttccgggaca atgtgaacaa tacccttggt tcgggcgatc
    23461 ctgtggcgcc gtccgccgcc gcgctcatta aatttggccc cggcacgctt gaactctccg
    23521 gcacgaacac cttccagggc aatgttcagg tcaaccaggg taccctcagc ctcactgctc
    23581 cgaatgtgat cccaaccttc gaaaacctga gtgcggtgac agggtccatt gtcaccatcc
    23641 gcccaggagc caccgtggcc cttaatggca acaatcagga gtttggaaac ttgaccggct
    23701 ccgtggtggt cagccctgtg ctgaacacag cgggtgtgct cgatctaggt tccgccacgc
    23761 tcaccgtggg gcggcagggc agcaacgccc tgttctccgg tcagatcacc ggcattgcag
    23821 gctctaaact gctcaaaatc ggcggcgggc gcctcactct cgacaattgg gatcctgcga
    23881 tgccgaactc cctcggcact ctcgaaattc tccagggggt tgtgcagtcc cgcctcaatg
    23941 acaactcctg ggcgcaggcc atcggctttg ccaactccat tcccgccagc acggagattc
    24001 ttctccgcgg cggcgagttt gaagcctatg tcgccggtga caacaccagc aacttccaat
    24061 tcatcccgca ggggcacagt ttcaccgcgc gtgacggcaa ttcgatcatt gacaccaacc
    24121 gctggcaggg aagtgcggcc aacaaaatca taggcttcaa caacctgacg gtggacaaga
    24181 atctgctcac gatcaccggt ggcaacacca tctatccgcg ttttgacggc acgttcacca
    24241 tgaccgccaa cgtgcgcctg caaatggacg ctcccggcat catcaacggg cccattacag
    24301 ggaactacac cctcaccaag acaggtgcca gcaacttgga ggtcaactcc agcaacgacg
    24361 cttggaatgg cggcgcggtg ctgcatgatg gtgtgctact cttcggctcg cgcactccgg
    24421 aggtggcgga acgctacatg gcgggcacca acctcttctc ctacagcgcc accgcaaacc
    24481 tgggcaccgg agacatcgtg gtgaatcgtg gcaccttgat ccgccttaat gccccttcca
    24541 atgtactcat ggatgacggg cagcgcgttc tcttgctggg cgacgcgcgc aacgccctgc
    24601 cgcgcgtgga catcggcctg gacgctccgt tgaccgacta ttccttgcgc tccaccggca
    24661 atggcgcgct gaccatcggc ctgaatgacg gtttttatac ccatgccatt gaccagtccc
    24721 tgcttggaaa cggccgctgg caggtcggcg cttgggcgac aaccttctac acggcgagca
    24781 gcatgggatc aggcgtggac aaccttttcc gctttggcgg agccaatggc ctgtttcaaa
    24841 tcactcaggg cggcgccctt tcaggcccct ccggtctttg ggtcggtgct gacatgctgc
    24901 ccaacggttt tgccattgcg aacggcaacg gacaggtgcg catcagtggc gaccaaagct
    24961 acactggcaa cacactcgtc ttccgcaacc gcgagggcgg cagcgtgcag aacttcctgg
    25021 aaatcctcgg cgacagcgcc tccccgactt tcgatgtcta tggtcgcctc atggctcgcg
    25081 gcgcgggccg cttcaccaat gatgcgggtg accaggtcaa caccgtcaat ctgcatccgg
    25141 gctccatcct gcgtctcgac tacgtcatgg atgtcaatga cactatgctc gtgtcccgtc
    25201 tggaaaactc caacctgggc cttgagtcca cggaaaacaa atggggtgac tccgcgccca
    25261 tgctgctcaa cggagccacc ctcaatctgg tcggctccag cggacgtgtg aaccgcgagg
    25321 tcattggcgt gctcagcgtc aaaggcggtg ccgctgtgct gctggagcgc aacaccacca
    25381 acggccagat catcctggag acgccctcca tcacccgcgt cggccaggcc accttcgatg
    25441 tgcgcgaaaa cgccgacgaa ctgggccggg tggaccttca aagccagaag ttttatattg
    25501 ataacggtgc gagcatgctc gacgcgcaaa acatgatgcc ggtctggatg atcaatccca
    25561 cccgcaacat gttcctgacc tacaacaacg acctcggggt tcagaaccgg gcgtttagca
    25621 acgagtttac caccggtgat gccacggccg ccgctacatt ccttggcggc ttgacttcct
    25681 ctagcatcgc ccgcttcgcc ggaggccagg gggatccaac gctagctggc acggtgaatg
    25741 tccatgccct ccgaattgac gccgttgctg acaatgagac aaccttcaca ggtggtcaga
    25801 tcaacatcca tagcggtggt ctcatcgcca acaacaacaa cgcggccgga cgcgtcaatt
    25861 tcaacaccac caatgtttat ttcggcgatg gctcggtggc gaaagagggt gtcatttatt
    25921 ccgccggcaa ccagatcacc actcgttttg gcggtgttgt gacagccgcc gacctgactc
    25981 tcaacggccc cggtcaggtt caactcacca atgtaaccaa tgccatcagc ggcaacatcc
    26041 agttgaacgg cggcaggctg ttccttgatg gggtaggcac agcgggcaca aacaccaccc
    26101 tcaccctgac cggcaactat gtgcaaaata acgacggcaa ccaaatgccg gaactactgc
    26161 tgcgcacact caatgccaac ggcgcctgga ccaacggcat catcgtcgcg gagcacactc
    26221 cctatgtgcg ggtcctggga cagtcgctgg acggcacatc attaacgacc gttcgcaacc
    26281 agaccatccc cagcctgatg atcgagggca cgaacactca acagggcacc accttcatct
    26341 tcggcacgac aagcaacacc aacaacgcca acaactacaa cttgattgtc agtggcgcga
    26401 ccacgctcgg cggcagcgcg cccatcggct tccgcattga gcgcgggggc accaacatcc
    26461 agggtggcgg cggcttcatg acgctggcgg gcgctgtcac cggcacggct ccagtcatca
    26521 aatcagggga cggctacctc gtgctccagg ccgataacac agggctcagc agtccggtga
    26581 ccctcaatcg tggcgaaata cgcggcgtgg gcaacaatgc caacaacttc ttcggcacgg
    26641 gcgattacca gttgaatttc ggcaccctgc gcatgagcac ggatgcggcc cgcacctatt
    26701 ttggagcgac tggacaggac ttgatcatcg gtggcgcggt aattcttgtg aatgaccgca
    26761 atggcggcgc ggcggcgcgc acacacacct ttggaaccaa caacggaaca aacaccattc
    26821 gaaccgagaa tggcgcgcac tggcgcatga catccgacag cagcgacagc gtggttttgg
    26881 agggcaaagt gatcgtcaat gattcggcga tgttttacac cgacaatgca cccactttcc
    26941 tccgggacac attcgagggg ggagggcgtt ttaccaagac aggcatttcg caggtgctct
    27001 ttgacaacaa tgtcgccaat tcggattgga aaggcgtgct tgatcttcag gcgggttttg
    27061 tgcgggttca gcagcccacc gcgacgctgg gtggggttgg cagttccatc atcatcaacc
    27121 caggggcggg cttgagtgtg cgcagtgtct ccaacttggg cacgggcttt ggcatcaccg
    27181 agctgcgtac aacctcgcgc accacggcca ctgtgatcgg ttccatgctt cccgccgatt
    27241 tcgtgagttt gaaaaatcac attcatggcc tgggcacgat aggtgacggt gtgggagttc
    27301 tcaccctcag cggcaaccag accttcacaa ccgacccagt catggcctca ttccgtgatg
    27361 gcaactggta cctcggtgcc atgcatgaca caggcacgct cagcgccaac agcgtctcgc
    27421 cctgggggcc gggtggaaac aagttccttc tgggaggcgg aagcgccacc ctcactttga
    27481 atccagccac tgccggcgcg caattcgcgg gaacgaatca aatggtgatc ggcattcctc
    27541 atgtcgtgta cggctatgcc accatggtct ttggggccaa ttccaacaac acctatgatg
    27601 gaggaacttt tgtgtctcgt tcacgcaacc tggacggcac ctatcgcggc actgttcttt
    27661 ccattcaggc cggacaggtc aacgccacaa cgtatcgcac ccctctcggc agcagtcttg
    27721 tggatgtgtt tggagaaatt cgcattgaaa acgtcaacgg caccgcgcgc aatgcggaca
    27781 ccgcaaacgc caacacctgg gtctttcaca aaggcacgcg ccttcgtttt gacaatgaca
    27841 acccgctcaa caccgcgagc accgagggac gctgggcgga caatgtcgcc atagccctga
    27901 acacgactgt tctggagatt tatggtgacg gcgccacgaa tgcctacaac accgagacgg
    27961 tcggagccat ctcggtggag ggaggctccg aggtcgtcgt gcgccgccgc ggcgcgttcc
    28021 tcgcggagat catcgcctcc ggcaacctga cccgcgtggg cactggtacc ctgatgctga
    28081 ccggcatgga caccaacaca aacacggtat ccggcctggg ttcgaacgcc gccacaaccg
    28141 cgatgcgctt caaggtggcc aatggcaacg atttgatgag caacaacatg gtggccccct
    28201 ggatcaccag ccgagtggat ggccagttcc tcaaatacac cgcggatggt tttgcgccaa
    28261 tcacgctggg tggttccccg gccaattatg tgtccattgt caatgcggct gccaccactc
    28321 tcacggcggg tggaaacgtg cctcttaatg atggaacaga aatcttcagc ctggaaggca
    28381 cgtccaacta cacccttgcc tccaatctgg acctctacgc gctgcgcctt gcgcgcgata
    28441 tcaatgtggc ggccaacggc acggccaaca acatcttcat ccgcagcggc ggtctcatcc
    28501 aggtggcaaa cactccgacc atcaacgcca acctgttctt tggtccggac gcccttgctg
    28561 acggcacgga tgtcggtgaa gccctcatct gggcttcgaa caacatcatt cagatcaacg
    28621 gcaagataca tgccagcaac gtgatcaaat ccggcacatc cgagctctac atccgcagcg
    28681 cccagccgca gtttacgggt gactgggtgg tcaacggcgg acgcatccgt ttcctgacac
    28741 ccggcgccca gggtggcggc gaaatcatcc tcaacggcag ccgcatgagt gatcgcgaca
    28801 acgtgcatac cattacggaa gtgctctacg gcttcaacag cggcagcccc gacctcttcg
    28861 attggaacgg cggcaaaatc actgcttatg attacaaccg cgtctttgcc aacaccgcca
    28921 gcgaccgcct tcagcagctt cccgccatgg atttgcgcac caccaacacg gttgccggca
    28981 ccggccagcc ggggctgctc ctttatcaag tggatggcaa ccgcaccacg gtgcgcaccg
    29041 gcacggttac cctctacgat cattatcagg ttcatgtcga gtccggcagc ttcggcaacg
    29101 gctccaccac gggcgtgcag ttcggctccg gctctggtgt gggaggtttg aacaaccagg
    29161 gccagttcga cctgcgcaag gtcggcgacg gtgtgctgat ccttggcgac aacagcctca
    29221 gctttaccgg caaccgcagc atcaccattg gcgagggcgc ggtgcgcgtg ctgcacaacg
    29281 gtgcctttgg tggggccggc aacaccggca acattgagca gggcggcgcc ctggaaatcg
    29341 ccgtggctgg ctggtctccc tccgccacac tcgtgcagca gcctggctcg gctgagcgct
    29401 gggccgtcaa cggagcgcgc agcggcaatg tgacactgcc ttccggcgtg catctgcaga
    29461 tcatgcacaa ccagaccggc acgcagacgg tgactctcga cggcggctcc atcatgggtt
    29521 acctgccgcg tgactgggat catgtggctg tcatccaccg tctgggctcc ggcgtcacca
    29581 tcaacctcgc ctccaactcg ttcctgggcc agccctatgt ggcctccaac aactcgttgt
    29641 gggataccgc gcgcatctat gacatcggca aattcaacca gaccaatgcc aacaaccctc
    29701 tcgatcaggg cctgcgcggc agctacctgc agattgacgg cgtgatcacc ggccccggcg
    29761 gcttgaccaa actgggccag gacatgatcg tgctcaatgg cgccaacacc tatgaggggg
    29821 cgacgatcgt cgaaaacggc atcctgcaga tcgggcgcaa caacgccctg cctgttggca
    29881 ccgacctgag cacggagacc agcagcggca tgtttgatct caacggctac aaccaggagg
    29941 tggcctcgct ctccggcgac cacggcagcg tcaacaacgg cgcgtttgac ttgaacaccc
    30001 tcaccctcaa ccagagcgcc agcaccacct acggcggcca gatcaacggc aacgtcagcg
    30061 tggtcaagag caacaccggc agcctgacac tgaccagtgc cagcggcatg aggggtgacc
    30121 tcgtcatcaa caacggctcc gtcatccttg gtgcggcggg ctccgtcaac gaggcgcgca
    30181 gcatccagat cggtgcgggc agcgtctttg acgtcagcgc cgtgaccggc ggctacagct
    30241 acgacggccg catcagcggc ggcggcgtgg gagcgctgag ggcggacaac gtcagccgcg
    30301 cgcagattct gggcagcatc accgtcaccg acaacgtggg cactatcgcg cggcagggca
    30361 gcatcagccc cggcaactcg gccgggcacc tgtatgtgag cggtgatctc acactgggcg
    30421 gcggtctctg gggcacgagc accaagaccg agcgcctgac cctcgagctg ggcgcgccga
    30481 cctccaccct ggcggcgctg ggctgggacg gcagcaacgt ggccgactgg ctggtcaacg
    30541 tctcgcctga cgtgctcaac gggctggcgg gcaacctcag cgggcacgat tatgtgaacg
    30601 tgggcggtga gctgacgctc aacgagcacg gcggcatcgg cgtgacgctc atcaacggct
    30661 accagccgca gtatggggac gtgttcaacc tgctggactg gacgagcgtg agcgtgggcg
    30721 gctttgacgc ggggccgaca ccgcgcagcg gcggggagct gggttacgac ctgaaccttc
    30781 ccgacttgac agccttccaa ctgacctggc acacggacct gttcgccgac tacggcgtga
    30841 tctttgtggt gccggagccg ggccgcatga tgctcctgtt cttcggcctc acgggcctgt
    30901 tcctcaggcg cagacgttga gcacgggatc atggaaaatc acacgggcat ccccgccgcg
    30961 ggatgcccgt tttcattccg cctcgatcca gaccgcgttc agggccagtc ttgcggccgc
    31021 cagcctgcct tctttcggcc ccagaatcat caaagcagtg gcaaatccgt cagcctggca
    31081 ggcgctttca tgaatcacgg tgactgaggc gagactgtgg tccaccgggc ggccggtgcg
    31141 cggatcgagc agatggttca cggagggatt ccccgccttg aagtgacggt aggtgccgct
    31201 ggtggccgcg cattgatcct tcaggcgcag ggtttgaaaa atgccgccat actccgcgcc
    31261 ggggccgcgt acaccgatat tccaaggctc tccatcaggg gccgtgccga tgcctgccac
    31321 ctcgccaccg atctccagca tgaagctgcg cagcccctcc gacttcaagc gcgtgatcaa
    31381 ctcatccatg acaaagccct ccgccacact ggccacattg atccgcaaac cgggcagggc
    31441 ttttctcaag gcgggcggct ccatgcgcca ggacagatga cgccagccgc agtgtgacat
    31501 ggccccggcc agctcggttt catccggcgg ccccgcccgc cgcgtgccgc caaaccccca
    31561 ggcatccacg agcggggcgc aggtgatgtc gagcacgtcg cctgtcttcc gcgcgatgtc
    31621 ccgcgcctcg ctgacgacgc gcgccagttc cgcgggcaca gcaaaccagg ccgtgctggt
    31681 ctggcggttg aatctggaaa gcgcggaatc ctcccgccaa tgggaaaaga ccgcctcgcg
    31741 ctcgtcgagg tgtgtttgaa tcagaagccg gtgcctctca ccggctcccg tcgcgcacag
    31801 gctccaggtc gtgcccatcg ttgagccgga gaactcgaca agtggcgcca cggcgctgcc
    31861 gtcttcagga tcgtcgctgc aggaggccag gacatgcgcc atcagggcga tgacgggcca
    31921 tagagcgcgg cgtttgtgtg gcaggcaggg cacggttcaa taggccgccg gcaggggcac
    31981 cgtcaaccac ccttgccctg ccgcttcagg tgcttgtcga aaaaaccgag caccagcggg
    32041 cgcaggtcgc gctggctggg ttgcaggtgg aaggtgtgcg gcgcgtccgg gatgatttcg
    32101 agatgatgct ccacgccgcc cgctttcaaa gcggcggcca gcgcctcgga gtcggccacg
    32161 gggacggtct tgtccgccgt gccatgcaaa atgagaaccg gcgggtcatc cttgtctatg
    32221 tgcgtccggg gatcggcctg cttgtagagt tccggcgcct ccgcgcggct tttgcggaac
    32281 atggcaatgt cccgctgctc gaaccacagc acaggcccgt acaaatccac cgcgcattgc
    32341 acgcgggtgg agccttcacc gggcgggtcc agttccggcc ctgtgagagc gaccatggcg
    32401 gccaggtgcc cgcccgccga gcctccaatg acgccgatgc gctccgggtc tatgcccagc
    32461 cgcgccgcgt tggcgcgcag ccagagcacc gcggtcttgc agtcgtgcag gttcttcggc
    32521 caggtgggtc cgggatgcgt tccatcggcc agcgcgtagt tgatgctcat gccgacatag
    32581 ccgttttggg ccagagtggt gccgatgttg atctcgcgcg ccgcgcgctt gtcgccgccg
    32641 gaccagccgc cgccgtggat gatgagcacg gcgggatgct tccgccccgc cttgggattc
    32701 gccggcaggt acaggtccgc cttttccgcg cgcccgccgc cgaggtagtc cacatcgtgc
    32761 tcggtgcgca cctcctgtgc ggggaggtcg aggcagaggg gaaggagaaa aaagagcgcg
    32821 gttctcattt tgcaatgcgt tgtgcggggc aggggagtca tcctcagact ttgtcgggaa
    32881 cctcgaagcc cttgcggtat tcgcgtttca gcatggcgag cgcttccggg gtggcggatg
    32941 cgccggtgaa ggtttcgttg gcggcgtcaa tctccagcaa ttccccgacg cggaggtcac
    33001 ccggcttgag ggtgacttcg ttggcggcga gatgggcggg cagatcctcg atgacgtgct
    33061 gccagggttc gaaatccgcg agccgcgcct ttgccatgtc gaggtcgcca tctttgccga
    33121 gacgccagga gagattgccc aggtggcacc aggcgctgga atagtggatc tgctcgatct
    33181 cagcgttgag gtccgtcttg ttccggctgc gcatggcttt gatgaaattc tccgcgtgct
    33241 gtccgccgcc gtcgcctttg aaacgctgga tgaccttgcc ctcgctgtca tgcgcggagc
    33301 cgccgccgcg cccgcccgcg tagtagccgc cctcgcattc gatgacgaga aaggcggtgg
    33361 tgcggcggcg cagatagacg tcgccggctt tcattccctt ctggcggggc atgttgtgga
    33421 tgtccatcac cacggggatg gtgcccgtgt ctatgtagag aaagtgtgtg ttcggagtct
    33481 cgcccgcgtc atcccagccg tagcgtccgc ctccggccag cacgcgcttc ggaaggctga
    33541 tcttgtcgcg gaagacgatg ttgcgcaggt cgtcaatgat gtgcggcccc cagtttccca
    33601 actcgccgtt gccggtgttc cagacccagt gccagtcgta gtggaacttg ggccggtaaa
    33661 tgggctcgtc ctgcgccggg ccgagccaga gattgtaatc caccgtcgcg ggcggtgtga
    33721 tgggcttttc ctctttgccg atgctctgcc tcatgccgta gtggttgagg cggacgtaat
    33781 tgattttgcc gagcgctcct gagtcgaggt aagctttgag ttcgaggtga aacggatcgc
    33841 tgcgctgctg ggtgcctccc tggacgatgc ggtcgtattt gcgtgcggcc tcgacgagtt
    33901 tgcggccttc ccagacattg tgggagacgg gtttttccac atacacatcc ttgcccgcct
    33961 ggcaggccca gatggaggcc agcacatgcc agtggttgcc cgtggagacg accacggcgt
    34021 ctgtttccgg atcctcgatc actttgcgca ggtcctgcgc cgttttggcc tgaggatatt
    34081 tctttttcgc gcgctcgatg acctccgtgt cggcgtcgca aagatacgcg atctcgacac
    34141 cggggatctt ggcgaaccag gcggcggatc ccatgccgcg gccgcccagg ccgatgaaac
    34201 caaggcggat tttttcattc gcccccagca cgcgcgaggc ggaaagggcg gtggcggcaa
    34261 aagcgctctg acggaggaag gtgcggcggt tgggcgtgtt catggcggtg tgagaatgcc
    34321 agggcaacgc gcgggaggca acccgtgttt cgcacggcgg cgccagtggg cggaaacaag
    34381 gccgcgctca gtcctccacg cccagccgca ctttgaggcg gttgatctgg gatttcagca
    34441 gctcgatctc cagttccatg cgctgtttcc aagcctggtc tccggcattg tcaggctgcg
    34501 ccgcgctcga aggggagatg atctcctcct gcggcgcgat gcctccaggc tcgccggtga
    34561 gcagttgcgc atactgcgcc acccgccgtc ccggtccggc tggcaggcag gccgcgaccg
    34621 ggccttcggg gtaggcgatg agacccgaaa gctcggcctc gacactttcc agatccggga
    34681 aggcctgaag gcgctcgctg cgcaggcgca gctctcccac cgtctgcgac ccgcgcagca
    34741 gcagcacggc cagcagggcg gccgcaggct tctccagacg gggcagtttg cctttgagat
    34801 tgtgccggta tttctggacg cgcgcgccga ccacggtgac ttggaaaacc cagcctcggg
    34861 atttcatgtt ctccagcgcc cgctgcacct cgccctcgtc caggcgcatg acgggctcgc
    34921 ggttggtgga ctggttgcag gcgctgacca gcgcgttaag cgtcaaagga tagtggtcag
    34981 gcagggtcag ctctttttcg atcaggcagc ccaggatgcg ggcttcgacg gcggtcagcg
    35041 gaggcggggc ggtgtcggtt tccatcgggg cgcaccatgc ctggtgacgc gcgcgcatca
    35101 aggcggaagg ctggcgctac cgggggaatg atggaggctc cagcgcgggg caggggcgtt
    35161 ttttttcgct cttgaaaccc accctccggg cggtaaagaa cgccctcccg cgcctggccg
    35221 cctctggcgt ttgaacccaa aaccccgccc caagctccca gatacccatc atgaacaaag
    35281 tcctcctcgc ccaagccgcc aatgaagccc gtggcctcgc catggacgcc gtccacaaat
    35341 gctcctccgg ccacctcggc ctgcccctcg gcgcggcgga catcggcgcg gtgctttttg
    35401 gcgaaagcct ccagtgcgac cccgccgacc cgaaatggct gaaccgcgac cgcttcatcc
    35461 tttccgccgg ccacggcagc atgttcatct acggctggct gcacctcagc ggctatgacg
    35521 tgagcattga tgacgtctcc aagttccgcg tcctgcacag catcacccca ggccacccag
    35581 agtttcacga aacccccggc gtggaatgca ccaccggccc gctcggccag ggcatcggca
    35641 acgccgtcgg ttatgccctc agcggcaaaa tggctgcggc gaaatacaac accgccgacc
    35701 acaagattat tgataatcac atcatcgccc tcgccggtga cggctgcctc caggaaggcg
    35761 tggcgcgcga agccgtggcc ttcgccgccc acaacggcct cgacaacctc atcgtcatct
    35821 ttgactccaa cgatgtcacc ctcgacgcca tggccaaggt cacgcagagc gaggacgccc
    35881 aggcgctcta caccgcgctc ggctgggacg ccgtgaccat tgacggccac gacctcgaag
    35941 ccatcaaggc cgccattgac accgccaagg ccgccgacaa cggcaagccc aagatcatca
    36001 tcgccaagac cgtcatcgcc aaaggcattc ccgaagtcgc cggcaccgcg aagggtcacg
    36061 gggaaggggg ggccaagttc attgatgccg ccaaaaaggg catcggtttg cccgaaggca
    36121 cgcatttcta cgtcagcgat gaagtgaagg gatacttcgc cgacctcaag gccaagcgcg
    36181 ccgccgccca cgccgaatgg aacaagagct tcgaagcctg gtccgccgcc aatcctgaga
    36241 aggccgccga actcaaagcc gcccgcgaag gcggcctgaa ggccgaagac ctgctcaagc
    36301 tcatacctga aacctccggc gaaggcaagg ccgccacgcg caacagcggc ggcgagatcc
    36361 tgaaccacat cgcaaaggcc gttccgcatc tcatcaccgg cagcgccgac ctcttcggct
    36421 ccacgaaaaa ctacatcaag gacggcggcg acttctccgc cgccaatcca accggacgca
    36481 acatctggtt cggcattcgc gagcacgcca tgggcgccat ctgcaacggc atcgcctacg
    36541 acggcctctt cctcgccagc ggcgcgacct tcctcgtctt cgccgactac tgccgcccga
    36601 gcatccgtct cgcctccttg gcgaagttgc ccgtcaccta catcttcacg cacgacagcg
    36661 tcggtgtcgg tgaggacggc cccacgcacc agcccgtcga aaccgtcagc ggcctgcgcg
    36721 tcattccgaa cctcgacgtt atccgccctg ccgacagcga ggaagccgcc gccgccttcg
    36781 ccgccgcctt cacccgcacc gacggcccca cgctcctctc cctcagccgc caggacctgc
    36841 cgcatcaaag ccacgccagc gccgccgccc gccgcgaagg cacgctgaaa ggcggttacg
    36901 tcctcgtcca agaaaccgcc ccgctggagg ccatcgtcat cgccactggc agcgaagtgc
    36961 agtgggccgt cgaaggagcc aaaggcaagc ccggcgtccg agtcgtcagc ctgccgtgct
    37021 tcgagcgctt cgaccgccag agcgccgagt atcgcgaaag cgtgctgccc gcgagctgca
    37081 ccaagcgcgt cgccatcgag gctggcgtct ccggcctctg gtggaaatac gtcggcaccc
    37141 agggccagat catcgccatt gaccgcttcg gcatcagcgc ccccggcaat ctagtcttca
    37201 aagaactcgg tattaccgcc gaagccgtgg cgtcagcgct tgcctgatcc agcgggatca
    37261 agtgcccgat tcacaaaacc tcccgtgtcg cgagtccggg aggtttttgc ttttccatga
    37321 cggttgtcac cggggtgtcc tcttgcgtaa atcttggaaa tgtccacccg cttctttacc
    37381 aaatccggtg agaacacgct gctcaaaaaa atcgcgggca tcttcaagca caacccggat
    37441 atcgagaggt ttgatgagct gattgtgtat cagcgtgctt cagagtctgt ccggcaaaag
    37501 gttcgacggt tgtaaagagg tgagtctctg ggactgaggc cgatgaaggc gatgcataca
    37561 aaggccccga gatggcgcgg gttcttcccg tggtagcgtg tgaggcgctt ggtgctctct
    37621 aggccgtgtt tgaaaacctg ctgattggcg gagttggaga gttgatgcag aagcaagcgc
    37681 cgatggaaac acaacgcttc gcatggtggg ggccgctcaa gcagcggctc aaaaattgcc
    37741 gccttgaagc cgggaggcag acccagggca ggattggaca gggtgcttga tgccttgttc
    37801 taccgtctgc ggaatgcgga tccttggcga gacctgcccg gttccgagca aatccaacag
    37861 caagctcgac aagaagctct gccgtcagcg ctgccggggg tggagaactt ctttcgccgc
    37921 ctcaaatcct gggcctgcgc cggcacacgt cgtgccagct cgcccgttac ttgttctgcc
    37981 tcatccagtt cgctgccatc atcggctggc tccggtgcgg ctgccgaggg ttttcaaaca
    38041 cggcctagcg ctttggctgc catttgggat actgctcctg cagcagtttg cggaattcgg
    38101 cggctttttc gggctggccg gctttgtcga gggcgcgggc ggctttgtcg agggcttccg
    38161 gagtgagttc ctcgtccacg agaaactggg cggggatcat gagcttgctg gcggcggcgc
    38221 tgtatttgtt gcgcgcctcg gcgtccgctc cgtcggcggc gtatttgtcg ccggcagaca
    38281 tggcgatgtc ggcctccaaa atgagcagca tggcctgggg cttgccgtcc ttggcaaagg
    38341 cgagcccgtc ctgtgcgatc ttttgcgcgt cctccagccg gccgagggcg agcagggcgc
    38401 ggcctttggt gacgagggcg cgggctttga cggcgctgtc cggggtgaca gccagatagt
    38461 ggtcgctggc ggcgatgctt ttttcaaagt cgcctgtttc aagcagcgcc atgccaagat
    38521 agttccagac gcgcgggtcg gtgttttgcg gctcgtcggg tgtggtggcc aggacgaggt
    38581 atctggcgga gcgggtgaac tcgcgcatgt catagagctt gagcccgagc caggtgagcg
    38641 tgttgagagg cacgacggcg ccggggctgg cctgcaagta ggcgtccacg gccttggcga
    38701 gggcgtccac attctgctgc gcgtagaggg ccaggatgat catctggctg gcgcggtcga
    38761 gataggtctg gcggtctttt tcgacggcct tttccaaagc gggcaccgcc ttgtcgaact
    38821 ccttctggtc gaagtgcgcc cggccgatgc cgaaccaggc ctgggcggcg gcggggctgt
    38881 tcgggtattt tttgaccagc atctcgaagg cggagatcat ggctttgaag tcgcgctgct
    38941 cgccggcgat gaggccgagc tggagatagg agacttcggc ggcgtcggag ttggtgaact
    39001 ctttgaccac gcgctggaag tctccgacag ccttggtgac atcgcgcgcg tcgcggtagg
    39061 cgaggccgcg gcgggcgagc gccttggcgg tgagggtgtg ggcggggtgc tcggcgagga
    39121 attgggagaa tgcggccgcg gcgtcctggt ggcgtccgga ctcggcgtag gaccagcctt
    39181 tgttgaagag ggccccggcg cggagcttct cgggcagggc ctcgttgagc acctctccga
    39241 agctaacggc ggccttggcg tagtcggctt ggttgaaata aaagtcggcg cggatgaggc
    39301 gcgcgaggtt catgaactcg tgatcgccat gcgccgcgga atgacgctgg atgaaggcgg
    39361 tggcgaagtc gccgagatcc ttgtcatcca ggaggtaaaa gcagtagagc ttccagtaac
    39421 cggcttcgaa ggcctgcaag gaggcgggga aatcctgctc gaccatgaga tagacctcga
    39481 ccgcgcggga gtagcttttc ttcatccggt aggcgttgcc cacaagcagg agcatcctgg
    39541 gccgggtctc gccctccggc aggacggtgg agttgcgggt gtagttgttg atgacgccgt
    39601 cgtagtcgcc tttggcaaag agcgcctgaa cgatgccgac gagggcgatg ccgcgattgg
    39661 cgggcgtcgt ctcggcgatg agcagggcgc gctcaaagag ggggacagcc tcgtcgggct
    39721 tgccggtccc ggcgagcagg acgccggctt tgacgcaggc ttcggagatg acggggttgt
    39781 ccttgctctc tttgacgagg tcgaggaaaa gcggcagggc gagttccttt ttgtcttccg
    39841 cgagatgcac ggtgccaagg ctgagaaggg cgttctcgcg gtaggggttg tctcctttga
    39901 cggcgagcac ggcctggagg gcggtgactt ggcggcgggt gtcccccagc tgctggtagg
    39961 agcgggcttt gttgaaggag gcggcgagcc gtacttgcgg aagcgggctt ttggtctcgc
    40021 agaaggcgaa atggcgggcg gactcggcga agttgcgcgc gttgaaggtg agggctccga
    40081 gccggtaggc ggcggagggg gctccatcgc tgttcgggaa gcggtccacc acctcgcggt
    40141 agtaggtctc ggccaccttg aggttgttca tcttcaaata gcactcgccg agccggaaca
    40201 tggccatggg cacatcggag ccggaggggt aggtttgaag atactggccg aaggattgcg
    40261 cggcgagcgt gtgctctccg cgctcgtaaa tcatggtggc gtagtcaaaa aggtcagtgt
    40321 cggggccttt tctccgcgcg ggagaggagg gcggaggtcc cacagggatg gcctttggga
    40381 tgggccgctc cccctcctcg acgggaatgg cgcgcggcac ctgctggctg cgcgcgccgt
    40441 gacaggcgag cgcggtgaga acggcaagaa tgcggagcat gtcggcgcgg cgtgtcatct
    40501 caattctgcg gggcttccgc atctggcttg cgggtggcga aggcgatgtt ccagatgccg
    40561 gccttctggc aggtgtccag cacgctgacg acattttgat aaggcacctg ctcatcgccg
    40621 cgcaggatga cagcctggtc tttgttgacg gcgacgatgt tggccagatt gcgcagaagc
    40681 tgggaggcgg tgacctcctg gccgttgacg atgatgacac cgtcctgctt gatgttgacg
    40741 atgatttccc cgacgttgcg cgcgcggtcg tccttgccgg tgtcagccgc gggcacgctg
    40801 acgtccatga tctgctcgac gctggcgtag tggctggtga cggcgaaaaa gataaccagc
    40861 agcaaaagca catccaccag cggggcgagc tgcatgggcg tgggttcgat ggagtgctgc
    40921 ttgcggaagt tcatggccgg tcagagaaaa aatcacatcg gctggccgtc cgggccgcgt
    40981 ccgctggcga cggcgcgggc ggcgcgcttg tattggacgg ccagcagggc catgaggtgg
    41041 gtggtggcgg cctccatttc ggaaataagg cggttcacct tgccgcggaa gatggcgtag
    41101 aaaatgaggg cggggatgcc gatggcgaga ccggaggcgg tgcagagcag cgcctcggag
    41161 acaccctggg ccaagccgag ctggctggag ccgccgtagt ccttggcgga gatggcgtgg
    41221 aaggtggtga tcatgcccag ggtggtgccg agcaggccga gcatgggggc cacggcgccc
    41281 acgtcgccga ggtaggcgat gcgcgcgagc agcaggctgg actggcggtt gccctcggct
    41341 tcggtgactt ctttgacctc ctcgaagctg gcggtggggt tgcgcgtggc gaagtcgagc
    41401 gtcttggcgg tgatgcgggc gatgcattcg ttgcgccggt tgcacacggc cagcaggccc
    41461 aggtagtcct gcttgcggat gagggcgtcg gcggagttca tgaaggcgtc gctgaccacg
    41521 gtgccctggc gcacggtgag ggcgaagagc acgatcaaga acgcggtgat gatggagagg
    41581 atgatcagcg ggtaggccat ggcgccggtg cgctcgatga agccgtccag tgtcagcgcg
    41641 cccgcgaagg ggttttcctc atccaccgcc gccgccggag cggccagcgt gaccgtttct
    41701 ccagacgcgg gcgcggcgtt ctgggcttgg agtccggcgc ctgaccagaa acaaagaatc
    41761 gccggcagaa caagggcgga gaggagtttt cggggcatga gtgagcggat tctaggcgcg
    41821 gcgggtgagc ttgcaaggag cgagatggac gcgcgccgcc gcctttgtgt tcacattctt
    41881 tcggtggtct ggatgccgag caggccgagg ccggccttga gcacgcggct ggcgccctcg
    41941 cacaatgcca gccgcgtggc gcgcgccgcg ccctccgctt tgaggacggg gcaggcctcg
    42001 tagaagctgt ggtaggtgtt cgccagttcg aacaaatagt tcgccagcag gttcgggcgg
    42061 tggtcggcca ggatgtcatg cacggtctcg gcgaactggg agagcttcag cgccagcgcg
    42121 cgctcggcgg gctcggtgat cgtcaatttc gcgccggcaa ggtccactgt gccgccctcc
    42181 aatttgcgga agatgctgcg cgtgcgcaca taggcgttga gcaggtaggg ggcggtgttc
    42241 ccctggaggg agagcatttt gtcccaactg aatttgtagt cggtgagccg gttctggctc
    42301 aactcggcgt atttgaccgc ggctgtgccg atgatgcggg cgatttcctc tttctcctcc
    42361 gcgctgtggc cctggtcgcg gctgtccacg gcttcgcgcg cgcgttcgat ggcttcctcg
    42421 atgacttcga gcaggcccac ggtctcgccg ctgcgggttt tgaacatctt gccgtccgga
    42481 cccaggatgc tgccgaaggc gatgtgcgtc atctccgtgc ccacgccgcg ccgcttggcc
    42541 gccgcgaaga cctggcggaa gtggagctgc tgcggcgcgc cgacgacgta ccaaatctcg
    42601 tccgcccgcc actcgcgtac gcggtagtct atcgttgcca ggtccgtggt ggcgtagaga
    42661 aagccgccgt cgcttttgcg gatgatgcac ggagcggcct tccatgagtc cttttccttg
    42721 atgagaaaag gatcgtcctc aggcttcaac gagccgtcgc tgaaaacgca gaccgcgccc
    42781 tcgctttcgg tggcgatgcc gctggcgagc atgtcctcga ccagcggcgc gagcgcgtcg
    42841 ttgtaaaagc tctcgcccag gtagtgatcg aagcggatgt cgagcgtggt gtaaaccttc
    42901 tccaactgct ccaaggtcag cctcacgcac tccttccaga tgccgaggtt ttccgcgtcg
    42961 ccctgctgca gtttcacgag ctcggctttg cagacttcga gcacggcatc gtcggccttg
    43021 gcctcggcgt tgacggtttt atagacgcgc accagctcgt ggatggcgtc ttttttcagc
    43081 gcctcccggt ccagctgcgt cttccagccg tggatgatca tgccgaactg cgtaccccag
    43141 tcgccgacat ggttgtcggt gatgacttcg tgccccagga aacgcgccac tcgggcgagc
    43201 gagtcgccga tgaaggtgct gcggatgtgg cccacatgca tgggcttggc gatgttcggc
    43261 gcggagaagt ccacgacgac gcgcttcggc cgggccgcct gcggcacgcc tgcgtgcgcg
    43321 tcccggatga tgaaggccag cctttgcgcc agggccgggg ctccgagggt gaaattcagg
    43381 aagccggggc cgtccacggt gatcttttca caaaggtccg cgcacacgct ttcgagcgcg
    43441 gccttgatct gctccgccag ggcgcgcggg ttcgtcttca actgcttcgc cagcaccatg
    43501 gcggcgttgc tttgatagtc gccgtagcgc gtgtccgagg cgatcacgac ggcgggggaa
    43561 aagccctccg gcgcggcgat gccggcggcg gtgaaggcgg cttgaagtcg gtcggtgagg
    43621 acggcgcgga acatggggcg cgttgtttac tcccgaagct cccagccttc aaccggcatt
    43681 gcttccaggc ggagccgcga ccgccgcgcg caggcagtca agccgcgggg gaggctgccg
    43741 gcgcgggcat ggcggcaggc tccgcgcatg aaggggcctt gccaaaaagg gtgcgcagct
    43801 cggccatgag ccagaggcgg atgccccagc gcaaaaaacg cagtgtgctg aagttcgggg
    43861 aaaagctcac tttgagtgtg aagtccatgc cgttgttcca tgcgccgtag gttcccttgg
    43921 cgaggcgcgt cttcgccagc ttgttgaggc ggcggttgta gaggctcatg aatttggcga
    43981 aaaaggtccc ggcaggcccg tcataaggca ggcgcgtcca ctcatagtcc gggctgtcat
    44041 agaccaggcg aacgggtccg atgaagtagg tggccaggtc catgatgaag gcgatgcgca
    44101 tcaattccgc atcccccaga tattcatatt tgcctttgta gagcgactcg aaccacagcc
    44161 ggtagctgcg tttgtaagcg cccttgaggt attccaccga ggcgctcaca tcctcgccct
    44221 ggacctcgcg gcggatcaga tcggtcacgg cgcagaccgt gtgcccgcaa tagtcaagac
    44281 cctggctgta aagcgggtct atgaagcccg ccgcgtcccc caccatggcc cagcggtggc
    44341 cggccatctg ctcggtgtgg taaggcaggc ccttgtagta aaaggtgtcg tcctccacgg
    44401 cctcggcctc ggcaaacatg agccgtccga tgggatgcgt caggaggtgg tccttcaacc
    44461 tttgcgtcag cgaggggccg ggaggcaggg tgaaaatgga gcggtcccac acaatgcccg
    44521 cgctgtagtc gtcattcgac aaagggatga gccagaccca ccagccgcgc ccggtgaggt
    44581 ggttggtggc tgtggctcgc aggcccttca cacgtttcat caagtccggg tgcgccatgc
    44641 ggctgcgggc gctgtcgagg tcgttgacgt tgcggaaacg gcaccaaaga gacgaggtgg
    44701 gatgctcatc ccccagcggg cggtgcaggc cgagctgctt ggcgagcagc gccgccttgc
    44761 cggaggcgtc aacgagccag cggcaggaat gctcggtctc cacgccgggg gcggtggtca
    44821 ctctgacggt gtgcattttc tcatcatcag cgagctggat gccgcgcagg gtggccgggc
    44881 gcagcagagc gcagccggcg cgctcagcct cgtcgagcac atgctggtcg agaatgccgc
    44941 ggtcaatctg gaaggtgggc aggcggcttt gaaatccggg accgatctcc gtgcagtcct
    45001 ccacccggtc atgcggcgtc ctgcagaacc acatgcgcag gccgtgcttt tgataatgcc
    45061 gtgagctgag gtgcgcgccc agatgcagca cgcgcgtgag aaagcaggcc ccgacctcgg
    45121 acgtgctctc acccaccttg cggctgaact cgcgcgtgcg ctccacgatc agcacccgca
    45181 gcccgggcaa agcgcgcttc agcagcagcc cgcaggccgc cccgctgatg ccgcctccga
    45241 tgatgatgac atcgtgctcg ctggggatgg ctcccctcaa agcgattctc acgccgcatc
    45301 cgaatgtgcg catcg
//
LOCUS       DAOMYJ010000015        44214 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Prosthecobacter sp. isolate AMR_MDS_5629
            NODE_336_length_44214_cov_23.183836, whole genome shotgun sequence.
ACCESSION   DAOMYJ010000015 DAOMYJ010000000
VERSION     DAOMYJ010000015.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867850
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prosthecobacter sp. (wastewater metagenome)
  ORGANISM  Prosthecobacter sp.
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Verrucomicrobiaceae; Prosthecobacter.
REFERENCE   1  (bases 1 to 44214)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 44214)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.30
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:34:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,742
            CDSs (total)                      :: 4,684
            Genes (coding)                    :: 4,676
            CDSs (with protein)               :: 4,676
            Genes (RNA)                       :: 58
            tRNAs                             :: 55
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..44214
                     /organism="Prosthecobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_336_length_44214_cov_23.183836"
                     /isolate="AMR_MDS_5629"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1965333"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..562
                     /locus_tag="PLP58_02815"
     CDS             <1..562
                     /locus_tag="PLP58_02815"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: protein motif:HMM:NF014200.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004185 - serine-type carboxypeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase"
                     /protein_id="HRJ07486.1"
                     /translation="QAGIRVAGGAASAREAAPMPEAGHEMWRHESPALADIVTSIHAT
                     SDNHETECVFRMIGAREKRPPAEVVRAHWQTRGLTFEGLRMEDGCGLARADHITPHDL
                     ARLQMLAATGPQGRVYADSLLAAHDGAVRYKGGAMSGVRSVAGLVTRPDGSRAAFALM
                     VNHFGNPRPYALLREAMLRALADVSD"
     gene            complement(597..1061)
                     /locus_tag="PLP58_02820"
     CDS             complement(597..1061)
                     /locus_tag="PLP58_02820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009958265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EVE domain-containing protein"
                     /protein_id="HRJ07487.1"
                     /translation="MRYWLLKSEPDVFSFADLKKRPRKTEPWNGVRNYQVRNMMRDHM
                     APGDLGFFYHSSCPQPGIAGIVRIVKEAYPDPTQFDPASEYFESGSRTENPRWLMVDV
                     KWEAGFKTFVSLDMLRSDPALAGMLILRRGNRLSITEVTEKEWKRICELGGL"
     gene            1144..1746
                     /locus_tag="PLP58_02825"
     CDS             1144..1746
                     /locus_tag="PLP58_02825"
                     /inference="COORDINATES: protein motif:HMM:NF013077.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase"
                     /protein_id="HRJ07488.1"
                     /translation="MSQLPSLDQTSALIRARRSVKPPDMDSARAVEPALLRELLENAT
                     WAPTHGLTEPWKFHVHQGAARARLAEALQRVYRETTPPAEFRADKLEKMGKNPLLAPV
                     VIACVMERRGAAKIPEMEEIEAMACALQNLMLSATAAGLASFWSSPPVLDAPAFKEWL
                     GIGVQDRCVALMYLGWPRPGMEPPRSARQPVETKILIHHD"
     gene            1739..2287
                     /locus_tag="PLP58_02830"
     CDS             1739..2287
                     /locus_tag="PLP58_02830"
                     /inference="COORDINATES: protein motif:HMM:NF016213.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF456 domain-containing protein"
                     /protein_id="HRJ07489.1"
                     /translation="MTEWLASLWEMLGGWWDAIPFMASGVWILTGCLLLTGLVGSVVP
                     LLPGPFLIFIAGIVHTLLLPEQGMGWQGIVALSLLLIIAYAVDTLAGALGARWFGASR
                     WGIAGVFVGGVVGLFFAPVGFLVGPLAGGLIFELIFAKKRMAPAVKSTWGTLLGTGLG
                     IVVRLVLSLAMIATVLVDCLWW"
     gene            2353..3720
                     /locus_tag="PLP58_02835"
     CDS             2353..3720
                     /locus_tag="PLP58_02835"
                     /inference="COORDINATES: protein motif:HMM:NF013567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HRJ07490.1"
                     /translation="MSSASNRRHFIKSAALGALAAPNILRAQNTPQKIRVGIVGIGGM
                     GAGAVQASSGEEIVAFCDADWRDLGARSAAGVAKKHPKVPKFTDFREMLEKKGAEMDA
                     VLISTPDHTHFAITMAAMQAGRHVFVQKPLAHNIWQVRTLQKAAKKYGVQTVMGNQGH
                     CWEGIRLVREWFEAGVLGEVREVHCWTDRPLAHMGFLNPQPTASPAAEPVPEGVAWDL
                     WQGPVAARDYNALYMPARWRGWWDYGCGGLGDIGCHCLDAPFWALRLGMPEKVEVLRQ
                     EAGALKSYTAPKSHIVYHFAARGEMPPVKIHWYEGGLLPEKLPGMEKDLPGNGMIMKG
                     SRETLFSDGMRVQSPQLWPRERMTPIVDLLRRRPLPRSKAGEPIGELFDAIRGDIPHA
                     GSHFDYACPLTEVVNVGVLAIRAGKSIEWDAPGMRVKDAPEFDAWIKEPVRDGWSYGE
                     DLWQA"
     gene            3737..4606
                     /locus_tag="PLP58_02840"
     CDS             3737..4606
                     /locus_tag="PLP58_02840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HRJ07491.1"
                     /translation="MILRLCLIALLCCAHARAQEIRRTPLVLTRGGTPEQPAVFDGKG
                     MVIDLGIDITGLEWARDGDLWTSRGPLPGHPPVTDTQRAGLFIEEIPLRIMRDRAAEQ
                     ESGEPGKVIHAAPDTLKPGEMTWTTDGSLCFRWPAGRSPGTARIIQPPAKLASCVVIA
                     CSHITVRNITAMHAANDGFNIHGHRVGIRLENVKAFSNGDEGISAHETVQMDVFDSEI
                     AWNGSSAGGVADVNDSVTTYTNCELHHNVNAAFFFDGKHHRVTNCLIHHQDKDIVIRG
                     DAVVEQSGNVWRK"
     gene            4697..6349
                     /locus_tag="PLP58_02845"
     CDS             4697..6349
                     /locus_tag="PLP58_02845"
                     /inference="COORDINATES: protein motif:HMM:TIGR01891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004180 - carboxypeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HRJ07492.1"
                     /translation="MKRNTLRRAAPAWPALGRKWLLACVAAPLLGADAAHAADLDSMK
                     AEALENVGSRSKLVREIMDSLFSFAEPGFQEFETQRYLCGILEDHGFKVGRGVAGMPS
                     AWVATWGSGKPVISLGTDVDALLGMSQKPGFAGEAPLVEGAPGHGEGHNSGMAAILPA
                     ALAVKELMEKHKLPGTLHIWPGIAEELLGGKAHYVRAGVFKDVDVVLFAHVGSSLSTW
                     WGDHRGKALVSVEYRFEGESAHAGGAPWKGRSALDAVELMNIGWNYRREHLRPQHRSH
                     YVITEGGGQPNVVPHRAAVWYYFRETDYDQVMYLWDIANRIAEGAAKMTDTRVSWRVL
                     GSAWPRYMNRALAGALHANIQQVGMPRWTEEDQALARAVQRIVEAGDQGLVTKTSSKL
                     EGVERIPDGLKHEGGSDDIGDVCWTVPTGHLSLPCNIPGVTMHHWSAAIAMATPIAHK
                     GAEAGAKVYALTILDLLAKPEIVRESREFFDNVQGRIRAYTSFLRPDDKPPVWLNHDL
                     MQRLRPAMRPLHFAPSKHSSYLDQLGISYPSLTPAGNSSPAK"
     gene            6440..9931
                     /locus_tag="PLP58_02850"
     CDS             6440..9931
                     /locus_tag="PLP58_02850"
                     /inference="COORDINATES: protein motif:HMM:NF024240.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HRJ07493.1"
                     /translation="MPRRNLLRLLACLAFPAWDLYAASPIPVVDLSQDTARHVIIAQG
                     TEKVYQGHPTTLLMPDGRTMFCVWTHGHGGTAGPLKRSDDGGKTWSEELPVPENWWKV
                     KNCPAIYRLTDPQGVSRLVVYAGQGLDGTMQQSVSTDEGKTWSPMQSNGLVCVMPFCT
                     IMPVEGGKKLIGLSNIRRPGETKDKKSNVVTQSESTDGGVTWSPWRILVDLGALVPCE
                     PEVIRSPDGKQLLCLMRENIRSEPAHFITSDDEGQTWSAVKALPPGLHGDRHKAVYAK
                     DGRLVVTFRDMGQGSPTRTHFVAWIGKYEDIVSGRDGEYKIKLLHSHKGSDCGYPGLE
                     LLPDGTFVATTYIKYRPGPEQNSVVSTRFTLAETDKSEKSTAAAAKSEALGIVLDDNA
                     AEYTGAWKMSDRHTPLVGATYRHDDRAKKSAAAAKFTPEIPADGNYEVRLLYVEASNR
                     AQKVQITIRSTDGEKVVTQNQREACLEDGIPRSLGVFAFAKGKSGSIEVSNVGADGYV
                     VVDGLQLVPEAIAERNTRADSGFPIKTSAAPVVIPPPMLLQSAAKPQEENGKSYDLVV
                     IGGTPGGVACAVRAAREGLSVLLVNHTQHLGGFVTSGAGGWEAPYDGARSPIYDEMLA
                     KAADYYRQTYGEGSPQHIASMPSKTSRAHIDRPKIEPRIAEMLFNQMVAKEKTLTVLL
                     GHIVTKAQRDGALLQSVTLKPMHGETTITVSGKIFADGMYEGDLMAAAGVKTQIGRES
                     RAQYGEKHAGVIYTQERHKDPGQRGFPKAADEGTLDIRYNSHATADIVEGPQSGEADG
                     SVMAYNYRLVLTRDPANRIMVTKPANFDPAIAKQAIGGGFVPNLPNGKVAWNGGRLIG
                     PQNEYPAADWPTREAISKRYLDAMLMRLWWVQNDPEAPEKDRKQFAGYGLAADEFPDN
                     GHAPYEIYVREARRLVGRYVFKEQDNVIAAGIARTPIYTDSIAMTDWPVDSVACLPRK
                     APGGNTDGILFLGEESRPAQVPYRSILANEVENLLVPVAISASHVGWGSIRLEPVWMQ
                     LGESAGFAAALAVKSQTTPAKLDPDVLIRKLATNRVMISFFNDVDVTSEDPRVPAAQY
                     FGTKGFFASYDAKLDVPLTEAVKTVWEKGFENLKKGTLEPMRLAKAVHEAEAQESPAA
                     KGTRGEALVAMWKSLAP"
     gene            complement(9958..10911)
                     /locus_tag="PLP58_02855"
     CDS             complement(9958..10911)
                     /locus_tag="PLP58_02855"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HRJ07494.1"
                     /translation="MSCLLPMSSRKPKPCLFVLDVGHGNAAVLHDDGGTVIFDTGRKG
                     TQISRHLASAGENRIEAMMLSHSDADHIGGAVTLLMDSKLTIGEVLLNPDPTKNSAVF
                     EQLCYALAEAIERSGTRVERRLATSTSLVRKGCRIDILHPPLVAALRGVGGDSKAGHG
                     HTSNSLSAAIRVSHGSNSSVLLAGDIEFDCVDGWKSLSVSPSATVLVFPHHGGLPGTA
                     SESDAGLFAYELVSLVRPTTVIFSNHRTKHNNPRDKVVSAITKAGPGIRCACTQLPER
                     YKSVVQSEKCWELHRSSSGVVEGTIKIELGVKDVFVGFEAS"
     gene            complement(10853..11575)
                     /locus_tag="PLP58_02860"
     CDS             complement(10853..11575)
                     /locus_tag="PLP58_02860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ07495.1"
                     /translation="MPSTALANRRIAIFEDNPRNRERLSDMVTRCGATAFPVIGPTPR
                     LNGLKTFLEHQSIQMVVCDHHLSQQRDYAPFLGAQAVAECYRLGIAGVLVTAFENTDA
                     ESSLRLFRRRIPALVRSPEDLDRTHLESALLSADREVRENNPPQERIPYRTIMTVQRI
                     ETLRSSKVVKVMMSQWDSAQEVGFPLDLVPKKLQAVVKPGSMLIAEVNIGAARQEDLY
                     FDKFELPAADVLKKAKTLFIRP"
     gene            complement(11577..13682)
                     /locus_tag="PLP58_02865"
     CDS             complement(11577..13682)
                     /locus_tag="PLP58_02865"
                     /inference="COORDINATES: protein motif:HMM:NF024977.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HRJ07496.1"
                     /translation="MPQTPPAQEPTSERLRFSTAVLRLLGEELNPSPDQGILELIKNS
                     YDADAKHCSVELKGVHIKGGSIVLKDDGVGMTAEEIRNGWLIVGDSMKSRSETSPSGR
                     LLVGSKGLGRLGALRLGRKVELTTRPKSEPGTQYRMEIDWAAFEHARVVEDVELAVTS
                     EKVPHSSHGTEITISQLPSPWRESDIKRLARAILLLRDPFAKERGFQAVLKAKEFTAL
                     ETLAQEGYFSESDFHLIAKIDQQGRASAEVLGAGGKKLFVGKHDDIADKKDAPAYKIP
                     ALSFQLWEFNLSKKGFAVASQQTTIQGLKAWLAQFGGVRLYHRGVRVMPYGEQKNDWL
                     DMNLKRSQHQELRPSTNNSSGCIRIEDPSGIFQQKTDRLGFIEGDALDTLRTFADNAL
                     SWMSGEREKARKLRLQAEAEAVRQHKNAAEKEMDEVIASLPPKEKQKVKKAAENVRSA
                     HEAEMKIKDETAQLYFTLGTVGTTAAAFAHQTKQPLRGIVADAKDLHNYLGNPDELLL
                     FREDSSRAVKRILLEANAIYSFSNVTLRLLEHEKRRSARHSIHQLIDETVELLSPYFE
                     ARETSLERDYANEELRVWCPRAAFEAIFTNLLTNALQAFEARAKLAANAGVRRIRVQT
                     RRSGDMAIIRVQDNGPGIEKLALEEIWLPGKTTTEKGTGLGLTIVKDVIEELRGHIEA
                     EAHGELGGASFTITIPLKS"
     gene            complement(13685..14983)
                     /locus_tag="PLP58_02870"
     CDS             complement(13685..14983)
                     /locus_tag="PLP58_02870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ07497.1"
                     /translation="MPKAVVHDFSGYRQFSAELHALALAPRVDAGWNKQVDASLDGVA
                     GKELQRLVDLTARRAAGAFFTGSTLAERLVGSLSFGKNAFIYDPTVGAADLLLAAARR
                     LPLGKSLRETLRRWGQCLAGTDLQPEFIEAARLRLALLARQSHGGDQKLPDDLSSFFP
                     HIRCANGLEQKQLLGRATHLLMNPPYGLVPAPADCAWAGGRVSEAAIFIVRLLEQASA
                     GTRLLAILPDVLRSGSFQHRWRERVGDLAKILKVERYGIFDDIADVDVFLLDVCRRSG
                     KKSDSHRWPVAVESADTKTIADFFDVHVGRVVPHRDKETGPLRRYIHPRSIPVWTDVR
                     RIEDKRHHPLAYQPPFVVLRRTSRPGHPYRAAATVILGKAPVAVENHLIVCEPRDKSA
                     DTCRALMAQLKTERVNEFLDERIRCRHLTVGAVKEIPVEF"
     gene            15255..15593
                     /locus_tag="PLP58_02875"
     CDS             15255..15593
                     /locus_tag="PLP58_02875"
                     /inference="COORDINATES: protein motif:HMM:NF024736.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HRJ07498.1"
                     /translation="MAKNYPTDVSDEEWALIKLEICALDKPRGKGRIASEASSRACFN
                     ALRYLLKTGCQWRMLPKEYPPRSTVHDALSRWTRNGVLERINGEQTRQRRELVKKTPS
                     PARRSSTAKA"
     gene            15590..16081
                     /locus_tag="PLP58_02880"
     CDS             15590..16081
                     /locus_tag="PLP58_02880"
                     /inference="COORDINATES: protein motif:HMM:NF033580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - transposition, DNA-mediated
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HRJ07499.1"
                     /translation="MKCADTVGTGEGGSGYDAGKKIKGRKRHILTDTQGLLLGVTVTP
                     ASVQDRDGAKVVCSVFCHQWQRLEVIFADGGYAGKLEGFIQQAAAGWGHAQGLRLEII
                     KRSEEAKGFVLLAKRWVVERTFGWLMKCRRLNRDQERDARHHESLIYLAMISLNLRIL
                     SKA"
     gene            16179..16331
                     /locus_tag="PLP58_02885"
     CDS             16179..16331
                     /locus_tag="PLP58_02885"
                     /inference="COORDINATES: protein motif:HMM:NF036995.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1016 N-terminal domain-containing protein"
                     /protein_id="HRJ07500.1"
                     /translation="MKARVRAAQVKAAVSVNRELILLYWHIGREILRAQKAEGWGAKV
                     VERCLR"
     gene            16420..17532
                     /locus_tag="PLP58_02890"
     CDS             16420..17532
                     /locus_tag="PLP58_02890"
                     /inference="COORDINATES: protein motif:HMM:NF014691.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HRJ07501.1"
                     /translation="MTYIHEWADWPKLTWDAAKLSALLADVRHRQGRLLGRMEGLGFR
                     LRSEAQLSTLTADVVKSSAIEGEKLDAEEVRSSIARRLGLEYAGAAVASRDVEGIVEM
                     MLDATQNHAQPLTAERLFGWHAALFLTGRSGMHKITVGAWRPAEAGAMRVVSGPIGRE
                     TVHFEAPSAEKLDQEMRAFLDWFEAANGVDPVVKAGVAHFWFVTIHPFEDGNGRIGRA
                     ITDLELARADGTAERFYSMSSQIETERKEYYLQLERGQRNGLDVTVWLEWFLGCLGRA
                     IAKADETLSGVLHKARLWEKVSQQTVNERQRKVINKLLDGFEGKLTSSKYAKLVKCSE
                     DTALRDIRALVERGVLIKNEAGGRSTSYTLATVACE"
     gene            17552..19096
                     /locus_tag="PLP58_02895"
     CDS             17552..19096
                     /locus_tag="PLP58_02895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ07502.1"
                     /translation="MNPFVHLACCLGALMLTAPLLSQEAALKRPPAPVLKKGEKQIFV
                     DSVMIRSKQGVTRVIHPAKKLERPVLTAEMPWEVRTEDGIIDKRVNIYGTVLRDEKSG
                     SFRMWYADPGSVLYATSSDGIHWERPVLNVKGATNETDLRVHSPSIIEDKFETDPAKR
                     YKAVGNASTGVDDAKLQRLKDKFEMVDWYRDKTQRLYYAAHSADGLHWKMDPEPILLG
                     CDTITLSQDPVTGEYLALHKRQGDPRVIGIRHIFLSVSKDMKHWSEPHPVHVADEVDN
                     KATRKLKGGTYSEYYNLSAFPYAGQWLGFVTHFRRVEPPSALFGNDEVNGRKRSATGI
                     IDVQLVTSRDGRHWHRCSDRSPVIPLGPHAYDAGSIFGLCNAPVIVGDEMWMYYTAST
                     TPHGGLAPEKEQSIARATWRIDGLASLQAKNKPGIIETHEFVPEGSKLTVNADVKSGS
                     IAVEVLDAAGQVIPGYEKEACMIEKRDSVKLAIRWKGTDALPAGKPVRLRFHLLNGDL
                     FSYWID"
     gene            19123..20352
                     /locus_tag="PLP58_02900"
     CDS             19123..20352
                     /locus_tag="PLP58_02900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ07503.1"
                     /translation="MKLLLLLSCLLLAPMLIHAQAKPRAQDYRIVFHNPDPERYVEGP
                     GLVRLDDGSLLAVVPVVPREEWSKERRATQSRTHILRSSDGGQSWAQVSELPYYSAAP
                     WIHQGELFLFANKGGTKVRNDDLLLLKSTDGGKTWSEPVTLFEGHFWNCHTGMVQKAD
                     RLYWATDDLALGKLRGPRVIAGDLSGDPMNPKAWRMSEPVPFLGVPEAMTNPQFDKLS
                     SQYLEPNVIEVRGKLRVMMTVKPKRQSTAGLAAVLDFEDKGGPIDLKFTQFHPMPGGQ
                     LKFCIIYDEKSKLFWATANAVVDGQGAYDWFREGEKRGNVRYASGLGGNDRRFLMLQY
                     SVDGLNWFQAGCVAQAPKISQSFMYARPVIDGDDLAIIARSSINAPNQHDADHATFHR
                     VNNFRSLALKLTPEPEE"
     gene            20402..21556
                     /locus_tag="PLP58_02905"
     CDS             20402..21556
                     /locus_tag="PLP58_02905"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialidase family protein"
                     /protein_id="HRJ07504.1"
                     /translation="MKLHLLFCVFVFSALNAAEPLLERTAVFPPGFNGIARYRIPGIA
                     VTTKGTVLAYCEARKNSSSDWGEIEVHLRRSTDGGKTWQEPKHIAHGGTRIEGNPHKK
                     DDAGGREQTVNNPVAVVDHETGAIEMLYCINYARCFSMRSTDDGVTWSAPVEITGTFE
                     PFRKHYDWKVIATGPGHGIQIKSGRLVVPIWLAYGKEGDHKPSAAATIYSDDHGKTWK
                     AGDLCLPNEGELGNPNETMITTLSDGRVMLVARSVSQASRKIITTSADGATGWTKPVF
                     HDQLWEPICMASIVAHPSKPGMLLFSNPHTLGLNKAGKPVPAGRGKRENLCIKLSRDD
                     GKTWPVNKTLDSGKAAYSDLAVLPDGTVLCLYEADTSIDCARFNLEWVTQ"
     gene            21680..22402
                     /locus_tag="PLP58_02910"
     CDS             21680..22402
                     /locus_tag="PLP58_02910"
                     /inference="COORDINATES: protein motif:HMM:NF015297.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase/citrate lyase family protein"
                     /protein_id="HRJ07505.1"
                     /translation="MNTPLRHPDLKLGTWLQTGSSVIAELADLSGFDWLLIDLEHGCG
                     TEAAVLPQIQVIRHAAAIVRVGAPHPEQIARALDWGAAGIMVPMVSTAEKAEACVRAM
                     RYPPLGDRGLAGMVRAFDFGLHAEKRTPVFHAQIETIEAVENARAIAAVDGVDVLFIG
                     PMDLKLNLQSRPGRTSLDFAACLREVAAAAKAAGKACGLLCRQTDDFAELQALGFTHL
                     AIETDITLLRESYRNVVQPLRV"
     gene            22405..26109
                     /locus_tag="PLP58_02915"
     CDS             22405..26109
                     /locus_tag="PLP58_02915"
                     /inference="COORDINATES: protein motif:HMM:NF019223.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1553 domain-containing protein"
                     /protein_id="HRJ07506.1"
                     /translation="MTLRTTYALLATFCVSALHADDSKPVLQLDFGQEESVPLIPVGH
                     VVRDQAGPRPPDFPDFEPNNTAIQLNGKGARFEIKDTGPQSRFDFTKGDAITLESWVK
                     VDQIGPGQPVYIIGKGRTNSPHFARNNQNWSLRVIGGSAGLAHLSFLFAGAPEAGGGN
                     TWHIWNSEASFKIATGWHHVALAYEFGKPDSMRGWIDGVATTGTWGVDGATTKAPVVD
                     DDDVWIGSSQGGNAGNSLVGFIDSIAIHRRILTDAEIAKHSRRKDGPEVVLPAVAEMP
                     DLGKIERGTVLVQFNEGAAASHRWPNSQEMPKEAARWTGDAFLLPRVPVRYDHWGIRS
                     SWEAPLLLRMAADVELPAGSRRVLLRTRGLSRLWIDGKLIAETKPAVANGENGFDPLT
                     PLAQPPHPGVRVRGYHQQEVFGTVTVPAKKTTSRVVLEVIVGGKNMRTDTGEVCVALE
                     SERGDAFSILHAADKPGLALTDAEVEPALVAIEASLARFDDASRLAASRSRDAFWQKR
                     HDAAKQWVKKNPPPKPPHEGHPVDAFLDAKIEKARAASAATTDEAKAFHSEVLPVLRE
                     QCFRCHGEKEKGGLKLNTREAALSGGDSEVPAILPGDPAASELIKRLRTNDEDLIMPP
                     TGDRLSGEKIARLESWIRNGAAWPSPPVDASRLAQTPLTSDATFLRRIYLDTIGLPPA
                     PDEVRAFLADTDPDKRRKVIDRLLADERWADHEMSVWLDALAENPTLINASLNSTGPF
                     RWFLHDALRDHKALDRMVTELLMMRGDAAHGGSAGFAQAAENDAPFAAKGHIVAAAFL
                     GIELQCARCHDSPYHSTLQRDLYSLAAMLERKAVTVPKTSRVPAAFFEKKARESLIRV
                     TLKPDELVTPVWPFASVTGVAEGQALDTLIENPQDTRERLAALVTAPENRRFPRVMVN
                     RIWKRLMGAGFVEPVQDWEGHDASHPELLDWLAAELISSGYDVRHIIRLIVTSQAYQR
                     EAGAEKWVSVAAEERFFSAPARRRLTAEQVVDSLHTAAGAVIDSEPMTFIHDGSKTLQ
                     TRQDLGRPRRAWMFASLNNERDRPSLALPRAQATVNVLEAFGWSGSRQKPIFDRDTDP
                     NMLQPGILANGTLTQSLSRAAWKSELADLAVEAASPEALLEELFLRFLSRMPLRAERE
                     SLLPALQAGFDTRLTPAGQIVEPAPLPPLRLVTWLNHVTSDANTIQQENENRAQRGPS
                     PDPRLRAEWREVYEDIIWSLINDRDFVWVP"
     gene            26148..27425
                     /locus_tag="PLP58_02920"
     CDS             26148..27425
                     /locus_tag="PLP58_02920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009961958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1501 domain-containing protein"
                     /protein_id="HRJ07507.1"
                     /translation="MKRRHFLKTSATLGGGLALPHLLFAESSAQPIRGKAEHVISIWL
                     GGGMGQTDTFDPKRLGDPKAKKAGSYYPSIETAVPGVRVCEHLSKMAPLMDRVTAVRT
                     VNHNVIDEHAAATNRMHTGRPISGTISYPSIGSIIANERGAVTEDAPPYVLIGYPNVT
                     RGPGFLGAQSSYLYLTDTSRGPAGLSLPPGISVQRQSRRERFLASLQASAGETQDKQL
                     KSYDDVIAQSLKLSGPEFNRAFQLDTEPADLRNQYGGEFGQRCLLSRRLVERGVRFIE
                     VSHNLNFINGAGWDTHKEAILQQHVLIKELDTAVAALIRDLEAKKLLDKTLIVITTEF
                     GRPPEFDSGGGRGHQGSAFSCVLAGGGLKHCGAWGETDELSKEIVANPVSVPDFFATI
                     CAAVGVDYRKNLYDGDRPVPVTDQGSPIAALFG"
     gene            complement(27692..29422)
                     /locus_tag="PLP58_02925"
     CDS             complement(27692..29422)
                     /locus_tag="PLP58_02925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009958936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase"
                     /protein_id="HRJ07508.1"
                     /translation="MRFLILLSALCPLLSAFSAEPMNVLFIAVDDLRPDIGCYGVKHA
                     KTPNIDRLAARGIVFDKAYCAQAVCSPSRTAILTGLRPDTTKVWDLNTHFRDAQPDCV
                     TLPQHFRQNGYHTSALGKIEHHGFEDGPSWTEPRWFPSGEMVKVDEKDWTKHTVTKFE
                     GVPSEFANPLEDVAKRKGGKAGKKGPSYEVSPKSDDELPDGAVAAEAVKRLAALKAAG
                     KPFFFGVGFVKPHLPFVAPKKYWDMHDPETIPGPAFEKYPEGTPDFVGHTNGELHAYP
                     GVPAGNPLPADFAKTLRHGYNACISFTDAQIGRVLDALDKEGLADNTVIVLWGDHGWQ
                     LGEHGLWHKHTNFEIAARSPLLIALPKSSTAGKHCAAPVEFVDVYPTLADVCGLTVPQ
                     GLAGMSLKPYLENPDAPMQKPAISIYPRSSKDHGGSLMGYSIRTEQWRCTFWRKRNAA
                     DIGFTELYDEQNDPNETVNLATKPEHAALIESLKKHLPPVGSDFAPKKSGKVAKTVGA
                     SKSKAYDPNEPRDKRYDRLYPGKPKLNETGYLAGQGGDKAEAKARFVKLDADKDGFVT
                     REEFISSGKK"
     gene            29514..30386
                     /locus_tag="PLP58_02930"
     CDS             29514..30386
                     /locus_tag="PLP58_02930"
                     /inference="COORDINATES: protein motif:HMM:NF013429.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HRJ07509.1"
                     /translation="MKRRVFVASLAAASLPSRASAVPELAGEIGITTGSFMRHLSADA
                     APGKLRLLDLPRRMSGELGLRVIDLMTATLAELTPAYCEKLRAEAGRCGCVLTNLKMN
                     QKGLDLGSPDKAVRDEALRVYKETIDLAAILGVRWVRPLPMLQRPDLSVYKDSYRALI
                     DYAKAKGIGVLVENFGWMQGDPDAIPRLIAVVGDDLKSQPDTGNWTGNDVRYLGLEKA
                     FPFAVSCDFKSRVLGPGGSHTEYDLKRCFEIGWKSGFRGPWCFEHFHQDLEQQFKEFA
                     LLRDMLRGWMNAAE"
     gene            30419..31744
                     /locus_tag="PLP58_02935"
     CDS             30419..31744
                     /locus_tag="PLP58_02935"
                     /inference="COORDINATES: protein motif:HMM:NF019222.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1552 domain-containing protein"
                     /protein_id="HRJ07510.1"
                     /translation="MIDRRNLLRQFAATAGAAFGTSLFPGIAQAAARSTSSGGPKRVI
                     FFLQNQGFDPKTCIPAGMKNSGSLARAKLPEPIEALEPFKERLHIINGLHGLHTSPSH
                     SAFFGALGGYRGDDGTPPLAPTIDYELSQILPETLLPHLCIGMDSIENMRAKPTIATL
                     SASGAGEPIFMHSNPNHLYQMLYGGISQGDIRKQHEARSGLFDRIEQMASAKGGALPT
                     GEQQRYGQFVQGFHDINGLRQRLGGVSDHLRQFAPKVDERYTKPKFETDWHDVLLDLG
                     ISALKSGITSVLTIGSGRGEIFGAWSGLGITEQGHNLGHMEQPDNPIWIKIRQYNCRM
                     LVKLMEQLESVPEGSGTMMDNTLIVYTSNNADKQHTSGATWPVMLLGNCNGVFKTGCF
                     TQLDGTRPINALYATLLKTVGVDRDRYNMSGKMAAKFDSGTGPLKELLA"
     gene            complement(31834..32391)
                     /locus_tag="PLP58_02940"
     CDS             complement(31834..32391)
                     /locus_tag="PLP58_02940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="HRJ07511.1"
                     /translation="MNAHPESILHAAESVVAVLKEFQTEAVVIGALALAAHHYVRFTE
                     DLDLGMNASAQKFRAIAEALRARGFHVELREPDADDPLSGVMDVMCPGGLIQLVNFGQ
                     TFPAVIEDALQAPPLTVRPESALRVVPLPHLVALKLYAGGFKSKADVVELLRRNTDAD
                     RDEILALCKRYRLRGLPELLEEAGK"
     gene            complement(32399..32581)
                     /locus_tag="PLP58_02945"
     CDS             complement(32399..32581)
                     /locus_tag="PLP58_02945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ07512.1"
                     /translation="MKSIAVPENHESLKPRASACAKASLNAELERSRLTTVEERILAA
                     LGLKTKFGWLEPSARK"
     gene            32641..35238
                     /locus_tag="PLP58_02950"
     CDS             32641..35238
                     /locus_tag="PLP58_02950"
                     /inference="COORDINATES: protein motif:HMM:NF019259.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1588 domain-containing protein"
                     /protein_id="HRJ07513.1"
                     /translation="MDTLPSLLLAAATLVCVSALAAETYTPGEPVRGGFKDFAHGFLE
                     QNCFECHDDETTKGDLNLLELGPVDETNTATWKSVWAQVTLGEMPPKKKKSQPQIIER
                     LRFSDWIVGQLGRVMKDKGGFHEPSDPQKGNFLRHELLFGPLPEGIRLVPTSSPARLW
                     RVTPQEHITRLNELINIEPPFDPKKPGLRTHGDAVPTNHGGELKLYFGTDNILQWVGG
                     TVAYATAVKSVPEVLSSLRNTGLENYPDLYSVNSAESTQILGMAEDILRFMAYGPRSL
                     VTFPEQITDDPKTYDKVKPKGDIRGLPNSIVYSTKVLRPMTPVADLMKEATLSDEKLL
                     AAVNWLFEALTFRPPTKEESGNYPAIVKDSIQKVGKEEGVFMGLSSLFLDRDALFRPE
                     LVEAGKPDAHGRVMLQDWELGLALNHALCFIKPDATLRSAITEGRMKTREDVRREVTR
                     MLDDDRIRKPRVLRFFREYFDHDMAGYVCKDARALAATGLSNRASIYYHDMFDATAST
                     DHMIELVLAEDKDVLKQLLTTQKVVATPRDKFYFGRFNSAEEVKEAIEAKKKADEAQE
                     AKENAQIAALKAEIAKLTAAVKAVPADKTAPRTLALKKKSLTAMETRFQEARAARKRG
                     DGENMKVTYAEFTGPETFARVTRRSFGDQSLTPERILATAPEGERLGILTHPAWLVSH
                     SDAMDNHAIHRGRWIRERLLGGGIPDVPITVDAMLPVEPESTLRERMRVTRETYCWTC
                     HQKMDPLGLPFEMYNHVGFFRTTELDKPVVTRGEIIDSGDSALDGPVTNALDMIAKLA
                     QSERVEQVFVRHAFRFWMGRNETLNDAPVLQAAHRAYRDNGGSMMALITSLVTSDAFL
                     YRKVERAAE"
     gene            35235..36608
                     /locus_tag="PLP58_02955"
     CDS             35235..36608
                     /locus_tag="PLP58_02955"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HRJ07514.1"
                     /translation="MSRHTLIAMPSRSLHALLIALGLALQSHAAPPNVIMLLVDDMGY
                     ADLGCMGAKDIRTPHIDRIAAEGVRMTDFYANAPVCSPTRAAFITGRWQQRCGIEWAI
                     GLTSEEEKRVNGALVPVKERFTMGLMPEDTFLVPLLKKNGYGTACYGKWHLGFKPEHN
                     PVKHGFDEFWGILGGQCDYYRYHYIDGMKQLYEKTEPATAEGYITDLINQRTVRYIRE
                     QSRQPFFIYVPYNAVHHPYQVPDDPTQFYTAENKNDGTRAGYAKMLERVDQGIGYIFA
                     ALEEKGILDNTLIIFTSDNGGERFSDNRLLFNHKTTLWEGGIRVPCLLRWPAGGLPKG
                     AVSTQTAITMDLTATLLAAVGLPVPPTMDGINLLPILSGKAAPIERTLCWRVDRTGRN
                     MKAIRHGEWKYVQDDLVEMLFNLREDISERHDLYHEHLDKVKDLKAKLAAWEAEVDKV
                     PPPIRIH"
     gene            complement(36732..38132)
                     /locus_tag="PLP58_02960"
     CDS             complement(36732..38132)
                     /locus_tag="PLP58_02960"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase"
                     /protein_id="HRJ07515.1"
                     /translation="MRILAILLALSSSLFAAQPNVLFILIDDFGSRDLGCYGSTLYET
                     PNMDRLAASGAKFTQAYVAYPRCVPSRFAIFTGKHPARVQGENDSPHVEPDRDATIGQ
                     AFKAAGYNTFYCGKWHLGEGASNPDQMGFDTIVAAGAAGATRSFFAPYTISKSKGHDE
                     KDAIVGLDDAPEGEYLTDRLTQETLKFIESSKDKPFCAVLAHYAVHTPIEAKKHLTKR
                     YEEKLATMPKAAVEWEKESAGENQLVQNNATYAAMIESVDNGLGRLLGQLKKLGIEDN
                     TIIVLASDHGGLSARGNNRGVATSNRPLRAGKGHLYEGGLRIPFLVRWPGNIKPGTEI
                     ATPVSTLDLMPTLAAMTGIALPNLAGTDGQNLVPLMQNGTAPARDTFYWHNPAPRPAS
                     TGDLFSSAIRVGDLKLLDFPGEKRLELYDLATDPGESKNLADERPTDRDRLLTKLNEW
                     RKEVGASMVEKVKKKK"
     gene            complement(38196..39137)
                     /locus_tag="PLP58_02965"
     CDS             complement(38196..39137)
                     /locus_tag="PLP58_02965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ07516.1"
                     /translation="MNSRAILSAALLLTASVLQTTAAEPKVVRVEFKPGATSAAIKGS
                     ITGEEFILYKLNARDGQYLSVSLQASTPSADFNIYIPGRGPGDDALFASAMAPEKKYS
                     GQLYKTGDHSISVFLNRNAARKGVTAKFDILFKITATPPDATTDAPVDDTAGFSQQAS
                     YGKMSFKVTSPAKESGNSFTLTPSGLSASNEPVTVPIKGFVVNLLCDDIDGDDSPEIA
                     VITQDGPQERGTAHVFSTNAKKSMSAVNLRDITDTPKLINGYGGGDEYQFVEGTFIRR
                     FPIQGDGAPTGKTRQLQYKLKPGEAMKQLVLDKSVEY"
     gene            complement(39220..39636)
                     /locus_tag="PLP58_02970"
     CDS             complement(39220..39636)
                     /locus_tag="PLP58_02970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ07517.1"
                     /translation="MRNNTLQILAITAIGLASLTLTQCVIDPAVLAELEKASQANSQG
                     SRPYYGQPYTVSVGQGAVTIMEGSRRVSTCYTASPNVENTRFISEQQQIVVKSRGNHG
                     PATVQLFDTRTGRQEGRVMAYEIRGGFPAWATGMGE"
     gene            complement(39674..40366)
                     /locus_tag="PLP58_02975"
     CDS             complement(39674..40366)
                     /locus_tag="PLP58_02975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ07518.1"
                     /translation="MFACLSGALGAEPTYGDPKLPVAGSVLGTTIHTRDAEELRYVVL
                     GRLLEAFAKEKDISVKAEEITAYRKAMEEGMAADRAEKQAAKNVLKRRMAAAGLPKTE
                     RQALEKELALIEQFLADTAPDKTPQTAEDKQALEQIASAFIKHWKVNRALQASYGGRI
                     GYQQGGPEPLDATRRFLEERKQRGDFTIASKALEDAFWSYYQNDSLHDFYKPGSKEET
                     QAFSSQPWAPKK"
     gene            complement(40449..40904)
                     /locus_tag="PLP58_02980"
     CDS             complement(40449..40904)
                     /locus_tag="PLP58_02980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ07519.1"
                     /translation="MIRQLSLTTAALLSLASCIVPVDPGGRPTQLPQRPGYSNQRPSS
                     SPGYVTQLPDRFPVGTAQGRISAEASQGAMIIRRNDGQVLCSFRTAMPNVETWRLVRG
                     DTQIIVKSRGARGPATVELFDTATGTLRDKVLAYAIRSSGASWAYGFED"
     gene            complement(41062..42369)
                     /locus_tag="PLP58_02985"
     CDS             complement(41062..42369)
                     /locus_tag="PLP58_02985"
                     /inference="COORDINATES: protein motif:HMM:NF016648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FecR domain-containing protein"
                     /protein_id="HRJ07520.1"
                     /translation="MSTEREKDFRWIESYLSGRATAEEVKALDARLSADAELRREFLR
                     YAHVDAALKTMVRPVSQQPLSAAKAKTTVWRSQTVRWAAAAVFLLAGVLALWMPRGVE
                     VRVMAVSDDAASIWPKDSTQRLREVRLEKGSVQLRLDSGVTLDLTAPVRLHLADAMHT
                     TLLAGSVTADVGERGHGFTIDTTSAHVVDLGTRFGVSLLDDGDTEVAVFQGKVEVSGP
                     KSAKDQRNLVTLSAGDSVRVNSVALIRRQALIATRGDSAFVLEPPPPDALVTGMTDNI
                     EEDGFHLFYTIQRGAMKPGVRPYSTLGRPRWLAMPGAEFPSELVGADVIGTFSADRYE
                     RDLRLEVSVSRACTVYVMLDTRGEVPEWVRRDFTDTGFRLRSGPWAGNPVVEGMTADA
                     NGEIHVDYTVWKKAVPAAGAITLGPPYPLGGATHRAMYGIAVK"
     gene            complement(42366..42899)
                     /locus_tag="PLP58_02990"
     CDS             complement(42366..42899)
                     /locus_tag="PLP58_02990"
                     /inference="COORDINATES: protein motif:HMM:TIGR02989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HRJ07521.1"
                     /translation="MPDPNFQQHANFLRLYVEHEPSIRGFVRSLVQTLVDADDVMQEV
                     AMALWKRFHTLSAPEDFRRWAFGVARLESLEFRRKMSRDRHVFGDELFATLADEAEEL
                     ADQFAKERTALDTCLQKLPADQRSLVEAAYAPGSRMDELAARLGRTAMAVYKSLHRIR
                     MMLTDCAKRELAKEGMA"
     gene            43016..43969
                     /locus_tag="PLP58_02995"
     CDS             43016..43969
                     /locus_tag="PLP58_02995"
                     /inference="COORDINATES: protein motif:HMM:NF019040.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1501 domain-containing protein"
                     /protein_id="HRJ07522.1"
                     /translation="MTFCFRFRSSFAAALTASLAFAGAGFAAEPYDAFLEKHCISCHG
                     PEKEKGDLRIDQLSRDFKLGADTHHWAEVIEQVNSGEMPPKKEKKPTQEEIAAFVTSL
                     DSRIKEGRAARMAARPAVAHYRLSRKEYQNTVYDLLGVRYDPAKPGELNEDTRWHGFE
                     RIGSELSLSPSHVDRYYRAAELVLERAFPAAAAGEARKLRKTGAEIRDLEAKKLLDKT
                     LIVITTEFGLPPEFDSDGGRGHQGSAFSCVLAGGGLRHCGAWGETDELSKEIVANPFS
                     VPDFFATICAAVGVDYGKNLYDGDRPVPVTDQGSPIEALFA"
     gene            44026..>44214
                     /locus_tag="PLP58_03000"
     CDS             44026..>44214
                     /locus_tag="PLP58_03000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ07523.1"
                     /translation="MLTAAQAIGTDGLTLDLVVPPGAKDRFQHAEFSFWIADASKPVK
                     VVIIHQHGCTNASPDKHPP"
BASE COUNT         8713 a        13517 c        13588 g         8396 t
ORIGIN      
        1 gcaggcgggc atccgggtcg cgggcggggc cgcgagcgca cgcgaggccg cgccgatgcc
       61 ggaagctggg catgaaatgt ggaggcatga gtcgcccgcg ctggcggaca tcgtcaccag
      121 catccatgcc acatctgaca accacgagac tgagtgcgtg ttccgcatga tcggcgcgcg
      181 ggaaaagcgc ccgcccgccg aagtcgtgcg cgctcactgg cagacgcgcg ggttgacctt
      241 cgaagggctg cgcatggagg acggctgcgg actggcgcgg gcggaccaca tcacgccgca
      301 tgacctggcg cggctgcaaa tgctcgcggc cacgggtccg caggggcggg tttatgcgga
      361 ttccctgctg gcggcgcatg acggggcggt gcgctacaag ggcggcgcca tgtccggtgt
      421 gcgcagtgtc gcggggctgg tcacgcgccc cgatggcagc agggcggcct ttgccctgat
      481 ggtcaaccac ttcggcaacc cgcgccctta cgcactcctg cgcgaggcca tgctgcgggc
      541 gctggcagat gtgagcgact gaaaagagaa ccggtcttca aaacccgcgc gcaggttcaa
      601 aggccgccca gctcgcagat gcgcttccac tccttttccg tgacctcggt gatggagagg
      661 cggttgccgc ggcgcaggat gagcatgccg gccagggcgg ggtcggagcg cagcatgtcg
      721 aggctgacga aggtcttgaa tccggcctcc catttgacat ccaccatgag ccagcgcggg
      781 ttttccgtcc ggctgcccga ttcgaagtat tcggaggccg ggtcgaactg ggtggggtcg
      841 ggataagctt ccttgacgat cctgacgatc cccgcgatgc cgggctgggg gcaactggag
      901 tggtaaaaga agccgagatc gccgggggcc atgtggtcgc gcatcatgtt gcgcacctgg
      961 tagttgcgca cgccgttcca cggctcggtt ttgcgcgggc gctttttcaa gtcggcgaag
     1021 gagaagacat cgggttcgga tttgagcagc caataacgca tgatggcgcg agcttggcgc
     1081 gcgcgggaat ttgttcaagc cggtcgtctt gacaaaaccg cgcgccgcgc gaaggaggcg
     1141 cggatgagcc aactgcccag cctcgaccag accagcgccc tgatccgcgc gcgccgctcg
     1201 gtgaagccgc cggacatgga ttccgcgcgc gcggtggagc cggcgcttct gcgcgagctg
     1261 ctggagaacg cgacgtgggc gccgacgcac gggctgacgg agccgtggaa atttcatgtc
     1321 catcagggtg cggcccgcgc gcggctggcg gaggccttgc agcgcgttta ccgcgagaca
     1381 acgccgcccg ccgagttccg cgcggacaag ctggaaaaga tgggcaaaaa cccgctcctg
     1441 gcgcccgtgg tgatcgcctg tgtgatggag cggcgcggcg cggcgaagat ccctgagatg
     1501 gaggagatcg aggccatggc ctgcgcgctg caaaatctca tgcttagcgc cactgcggcg
     1561 gggctggcct ccttctggtc ctctcctccc gtgctcgacg cgccggcctt caaagagtgg
     1621 ctcggcatcg gggtgcagga ccggtgcgtg gcgctgatgt acctgggctg gccccgcccc
     1681 ggcatggagc cgccgcgcag cgcgcgccag ccggtggaaa cgaaaatcct gattcatcat
     1741 gactgagtgg ctggccagtc tctgggaaat gctcggcgga tggtgggatg ccattccgtt
     1801 catggcgtca ggcgtctgga ttctcaccgg ctgcctgctg ctgacaggcc tggtcggctc
     1861 ggtggtgccg ctgctgccgg ggccgtttct cattttcatc gcgggcatcg tccacacgct
     1921 gctgctgccg gagcagggca tgggctggca gggcatcgtg gcgctcagcc tgctgctcat
     1981 catcgcctac gcggtggaca ctctggcggg ggcgctgggc gcgcgctggt tcggcgccag
     2041 ccggtggggt atcgcgggag tgttcgtggg gggcgtcgtg ggtttgtttt tcgcgccggt
     2101 gggtttcctg gtcgggccgc tggcgggcgg gttgattttt gagctgatct tcgccaaaaa
     2161 acgcatggcg ccggcggtga agtccacctg gggcacgctg ctgggcacgg gattgggaat
     2221 cgtggtgcgc ctggtcctga gcctggcgat gatcgccacc gtgcttgtgg actgcctgtg
     2281 gtggtgagcg cgcgtcattt cgtccaaaga gcggcgagtt ttccgccttc cgcggcgttc
     2341 cctccatccc ttatgtcatc cgcatccaac cgccgtcatt tcatcaaatc cgccgctctc
     2401 ggcgctctcg ccgctccaaa catcctgcgc gcgcaaaaca cgccccagaa aatccgggtc
     2461 ggcattgtcg gcatcggcgg catgggggcc ggcgcggtgc aggcctcatc cggggaggag
     2521 atcgtggcgt tctgcgatgc cgactggcgt gatctgggcg cgcgcagcgc cgccggcgtg
     2581 gcgaaaaaac atccgaaggt gccgaagttc accgacttcc gagagatgct ggagaagaag
     2641 ggcgcggaga tggacgcggt gctcatctcc acgccggacc acacgcattt cgccatcacc
     2701 atggcggcca tgcaggcggg caggcatgtc tttgttcaga agccgctggc gcacaacatc
     2761 tggcaggtgc gcacgctgca aaaggccgcc aaaaaatacg gcgtgcagac ggtgatggga
     2821 aaccagggcc actgctggga gggcatccgc ctcgtgcgcg agtggttcga ggcgggcgtg
     2881 ctcggcgagg tgcgcgaggt gcattgctgg acggaccgcc cgctggcgca catgggcttt
     2941 ttgaatccgc aacccaccgc cagcccggcc gccgaacccg tgcccgaggg cgtggcctgg
     3001 gacctgtggc agggacccgt ggccgcgcgc gactacaacg cgctctacat gcccgcgcgc
     3061 tggcgcggct ggtgggacta cggctgcggc ggcctggggg acatcggctg ccattgcctc
     3121 gacgcgccct tctgggcgct gcgcctcggc atgccggaga aggtggaggt gctgcgccag
     3181 gaggccggcg cgctgaaaag ctacaccgcg ccgaagtcgc acatcgtcta tcacttcgcc
     3241 gcgcgcggtg agatgccgcc ggtgaaaatc cactggtatg agggcggcct gctgccggaa
     3301 aaactccccg gcatggaaaa agacctccct ggcaacggca tgatcatgaa gggcagccgc
     3361 gagacgctct tctccgacgg catgagggtg caaagcccgc agctctggcc gcgcgagcgc
     3421 atgacgccca tcgtggacct gctgcgccgc aggccgctgc cgcgctcgaa ggcgggcgag
     3481 cccatcggcg agttgttcga cgccatccgc ggggacattc cccatgccgg ctcgcacttc
     3541 gactatgcct gcccgctcac cgaggtggtc aatgtgggcg tgctcgccat ccgcgcgggt
     3601 aaatccatcg aatgggacgc ccccggcatg cgcgtgaagg acgcgcccga attcgatgcc
     3661 tggatcaagg agccggtccg cgacggctgg agctacggcg aggacctctg gcaagcgtga
     3721 tcaccccaac gcccgcatga tcctccgtct ctgcctcatc gcactgctat gctgcgctca
     3781 tgcccgcgca caggaaatcc gccgcacgcc gctcgtgctc acccggggcg gcacgccgga
     3841 gcagcccgcc gtctttgatg gaaaaggcat ggtcatcgac ctcggcattg acatcaccgg
     3901 actcgaatgg gcgcgcgacg gcgatctgtg gacctcgcgc ggtcctttgc ccggacaccc
     3961 gcccgtgacg gacacgcagc gcgccggact cttcatcgag gagatccccc tgcgcatcat
     4021 gcgcgaccga gccgccgaac aagaaagcgg cgagcctggc aaagtcatcc acgccgcgcc
     4081 ggacacgctc aagcccggcg agatgacctg gaccaccgac ggctcgcttt gctttcgctg
     4141 gcctgctggc aggtcccctg gcaccgcgcg catcatccag ccacccgcaa agttagcgag
     4201 ctgcgtcgtc atcgcctgct cgcacatcac agtcaggaac atcaccgcga tgcacgccgc
     4261 gaacgatggc ttcaacatcc acggtcatcg cgttggcatc cgcctcgaaa acgtgaaggc
     4321 attctccaac ggcgacgagg gcatcagcgc gcatgagacc gtgcagatgg acgtctttga
     4381 ttccgagatc gcctggaacg gctccagcgc cggcggcgtg gccgatgtga acgacagcgt
     4441 cacgacttac accaactgcg agctgcatca caatgtgaac gccgccttct tcttcgacgg
     4501 caaacatcac cgcgtcacca actgcctcat ccaccatcag gacaaggaca tcgtcattcg
     4561 cggagatgcc gtggtggagc agtccggcaa tgtctggcgc aaatgagccg ctcccgcgct
     4621 ccatgagccg cgcagtctgt gaaaacgcct cattgagtct tgacgccgca agcgcgggcg
     4681 gggcatgatg cccgtcatga agcgaaacac actccgccgc gcggctccgg catggccggc
     4741 acttggaagg aaatggctgc tggcctgcgt cgcggcgccg ctgctgggcg cggatgcggc
     4801 gcatgcggcg gacctcgaca gcatgaaggc ggaggcgctc gaaaacgtcg gctcgcgttc
     4861 gaagctggtg cgcgagatca tggacagcct gtttagcttt gccgagccgg gtttccagga
     4921 gttcgagacg cagcgttacc tctgcggaat ccttgaggac cacggattca aggtcgggcg
     4981 cggagtggcc ggaatgccct ccgcatgggt ggcgacgtgg ggctccggca agccggtcat
     5041 ctctctcggc accgatgtgg acgcgctgct cggcatgtcg cagaaacccg gcttcgccgg
     5101 cgaggcgccg ctggtggagg gcgcaccggg tcatggcgag gggcacaact ccggcatggc
     5161 cgccatactg ccggcggccc tggccgtgaa ggagctgatg gaaaaacaca agctgcccgg
     5221 cacgctgcac atctggcccg gcatcgccga ggaactgctc ggcggaaagg cccactatgt
     5281 gcgggcggga gtcttcaagg atgtggacgt cgtgcttttc gcccatgtcg gctccagcct
     5341 ctcgacctgg tggggagatc accgcggcaa ggcgctggtc tcggtcgaat accgcttcga
     5401 gggcgagtcc gctcacgcgg gcggcgcccc gtggaaagga cgcagcgccc tggacgctgt
     5461 ggagctgatg aacatcggct ggaactaccg ccgcgagcac ctgcgccccc agcaccgctc
     5521 gcattatgtg atcaccgaag gcggcggcca gcccaacgtg gtgccgcacc gcgccgcggt
     5581 ttggtattac ttccgcgaga cggattacga ccaggtcatg tacttgtggg acatcgccaa
     5641 ccgcatcgcc gagggagcgg caaaaatgac cgacacccgt gtgtcatggc gcgtgctcgg
     5701 ctccgcatgg ccgcgctata tgaaccgcgc cctcgcaggc gcgctccacg cgaatatcca
     5761 gcaggtcggc atgccgcgct ggacggagga ggatcaggcg ctggcgcgcg ccgtgcagcg
     5821 catcgtggag gcgggtgacc aggggctcgt caccaagacc agctccaagc tcgagggtgt
     5881 cgaacgcatc ccggacggcc tcaaacacga gggaggctcc gacgacatcg gcgatgtctg
     5941 ctggacggtg ccgacagggc atctctccct gccgtgcaac atccccggag tcaccatgca
     6001 ccactggagc gctgccatcg ccatggccac gcccatcgcc cacaaaggcg ccgaggccgg
     6061 cgccaaggtc tatgcgctca ccattctcga cctcctcgcc aagccggaga tcgtgcgcga
     6121 gtcgcgcgaa ttttttgaca acgtgcaggg ccgcatccgc gcctacacat ccttccttcg
     6181 tccggatgac aagcctcccg tctggctcaa ccacgacctc atgcaacggc tgcgtcccgc
     6241 catgcgcccg cttcatttcg ccccttccaa acactcatcc tatctcgatc agttgggcat
     6301 ctcctaccct tcactcacac ccgcgggcaa ttcttcgcct gcgaagtgac gccccgttct
     6361 cgtgcggcgg taatccgcgc gcggcatggt catctcaact gaccagaaaa ccacactccg
     6421 gaagattcgt gtcctaatca tgccccgccg aaaccttctc cgcctcttag cctgcctcgc
     6481 cttccccgcc tgggacctct atgcagccag tccgattcca gtcgttgatc tttcccagga
     6541 caccgcgcgc cacgtcatca tcgcccaggg cacggagaag gtttatcaag gccatccgac
     6601 gacgctgcta atgccggatg gcaggacgat gttctgcgtg tggacgcacg ggcatggcgg
     6661 cacggcgggg ccgctgaagc gcagcgatga cggcggcaaa acgtggagcg aggagctgcc
     6721 ggtgccggag aactggtgga aggtgaagaa ctgtccggcg atctaccgcc tcaccgatcc
     6781 gcagggcgtg tcgcggctcg tcgtgtatgc cggtcagggg ctggatggca cgatgcagca
     6841 gtcggtttcc accgacgaag gcaaaacgtg gtcgccgatg cagagcaacg gcctcgtgtg
     6901 cgtgatgccc ttctgcacga tcatgccggt ggagggcggg aagaagctca ttggcctctc
     6961 gaacatccgc aggcccggtg aaacaaagga caagaagtcg aatgtcgtca cgcagagcga
     7021 atccacggac ggcggcgtca cctggagccc gtggcgtatc ctggtcgatc tcggcgctct
     7081 cgtgccctgc gagccggagg tgattcgctc gccagatggc aaacaacttc tttgcctgat
     7141 gcgcgaaaac atccgcagcg agcccgcgca cttcatcacg agtgatgacg aaggccaaac
     7201 ctggtcggcg gtgaaggcgc tgccgcccgg actgcacggc gaccgccaca aggcggtcta
     7261 tgcgaaggac ggaaggctcg tcgtcacctt ccgcgacatg ggccagggca gccccacgcg
     7321 cacgcatttc gtcgcgtgga tcggcaaata cgaggacatc gtcagcggcc gtgacggcga
     7381 atacaaaatc aaactgctgc acagccacaa aggcagcgac tgcggctatc cgggcctcga
     7441 actgctgccc gacggcacct tcgtcgcaac gacctacatc aagtatcgcc ccgggccgga
     7501 acagaactcg gtggtgagca cgcgcttcac gctcgcggag acggacaagt cggaaaagag
     7561 taccgcagca gccgcgaaat ccgaagcgct cggcatcgtg ctcgacgaca acgcggcgga
     7621 atacaccgga gcatggaaga tgtctgacag gcacacgcca ctcgttggcg caacgtatcg
     7681 ccacgatgat cgcgcgaaga aatcggccgc ggcggcgaag ttcacgcccg agattccagc
     7741 ggatggcaac tacgaggtac gtttgcttta cgtcgaggcc tcaaatcgcg cgcaaaaggt
     7801 gcaaatcacg atccgcagca ccgatggcga gaaagtcgtg acacaaaatc aacgcgaagc
     7861 ctgtctggag gacggcatcc cgcgctcgct cggcgtgttt gccttcgcga agggcaaatc
     7921 gggcagcatc gaggtttcca acgtgggggc ggacggctat gtcgtcgtcg atggcctgca
     7981 actcgttccc gaggccatcg ccgagcgcaa cacccgcgcc gactcaggct ttccgatcaa
     8041 gaccagcgcc gcgcctgtcg tcatcccacc gcccatgctt ttgcaaagcg ccgccaaacc
     8101 gcaagaagag aacggcaaat cgtatgacct cgtcgtcatc ggtggcacgc cgggcggcgt
     8161 cgcctgcgcg gtgcgtgcgg cgcgcgaggg actcagcgtg ctgctggtga atcacacgca
     8221 gcacctcggc ggcttcgtca cgagtggcgc aggcggctgg gaggcaccgt atgatggagc
     8281 gcgctcgccc atttatgacg agatgctcgc caaagctgcg gactactatc gccaaactta
     8341 tggcgaaggc tcgccgcagc acatcgcttc gatgccgagc aagacgagtc gcgctcacat
     8401 cgaccgcccg aagatcgagc cgcgcatcgc cgagatgcta ttcaatcaga tggtcgcgaa
     8461 agagaagacg ctgaccgtgt tgctcggcca catcgtcacc aaggcgcagc gcgatggcgc
     8521 tttgctccaa agcgtgacgc tcaagccgat gcatggcgag acgacgatca cggtgagcgg
     8581 caagattttc gccgatggca tgtatgaggg cgatttgatg gccgccgcgg gcgtgaaaac
     8641 gcaaatcggc cgcgaatcgc gtgcgcaata cggcgagaag cacgcgggcg tcatctacac
     8701 gcaggaacgc cacaaggatc ccggtcagcg cggctttccg aaggccgccg acgaaggcac
     8761 gctggacatc cgctacaaca gccacgccac cgccgacatc gtcgaaggcc cgcaaagcgg
     8821 cgaggccgat ggcagcgtaa tggcctacaa ctaccgcctc gtcctcacac gcgatccagc
     8881 gaatcgaatc atggtgacca agcccgccaa cttcgatccc gccatcgcca agcaggccat
     8941 cggtggcggt ttcgtgccaa acctgccgaa tggcaaagtc gcgtggaacg gcggccgact
     9001 catcggcccg caaaacgagt atcccgccgc cgactggccc acgcgcgagg cgatctcgaa
     9061 gcgctacctc gacgccatgc tcatgcgcct ctggtgggtg caaaacgatc ccgaagcgcc
     9121 ggagaaggat cggaagcagt tcgctggcta cggcctcgct gccgatgagt tccccgacaa
     9181 cggccatgcg ccgtatgaaa tctacgtccg cgaggcacgg cggcttgtgg ggcgttatgt
     9241 cttcaaagag caggacaacg tcatcgccgc aggcatcgcc cgcacaccga tctacaccga
     9301 cagcatcgcc atgaccgact ggccagtcga ttccgtcgcc tgcctgccgc gcaaggcacc
     9361 cggcggcaac accgacggca ttctcttcct cggggaagaa tcccgccccg cgcaggtgcc
     9421 gtatcgctcg atcctcgcga acgaagtcga gaacctcctc gtccccgtcg ccatcagcgc
     9481 ctcccacgtc ggctggggca gcatccgcct cgaacccgtc tggatgcaac tcggcgaaag
     9541 cgccggtttc gccgccgcac tggccgtgaa atcccaaaca actcccgcga agctcgatcc
     9601 cgatgtgttg atccgaaaac tcgccaccaa ccgcgtgatg atcagcttct tcaacgatgt
     9661 cgatgtcacc tccgaagatc cccgcgtccc cgccgcgcag tatttcggca cgaaaggctt
     9721 ctttgcgagt tacgatgcca aactcgacgt gccgctcacc gaggccgtga aaacggtatg
     9781 ggagaagggc ttcgagaatc tcaaaaaagg cacgctggag ccgatgcggc tcgcgaaggc
     9841 tgttcatgag gctgaggcgc aggagtcacc ggccgcgaaa ggaacgcgag gtgaagctct
     9901 cgtcgccatg tggaagtcgc tcgcgccgtg aacctacggg gggttcagaa gctcaagcta
     9961 cgacgcctca aatccgacga acacatcttt tactccaagt tcgattttga tcgtgccttc
    10021 tacaacaccg ctgctactgc ggtgcagttc ccagcacttc tctgactgaa ccacggactt
    10081 gtatcgttcg ggcagttgtg tgcaggcaca ccgaatacct ggaccggctt tggtaatggc
    10141 actcaccacc ttgtcgcggg ggttgttgtg cttggttctg tgattggaaa aaatcacagt
    10201 tgttgggcgg accagtgaca ccagttcata cgcaaaaaga ccggcatcgg actcggaggc
    10261 tgtgccggga agtccgccat gatgcgggaa aactagcacc gttgcagatg gagagactga
    10321 gaggctcttc cagccatcaa cgcaatcaaa ctcaatgtct ccagcgagca atacggagga
    10381 gttagaccca tgcgaaacac gaattgccgc actcaatgaa ttcgaagtgt ggccatgccc
    10441 agccttcgag tctcctccaa cacctctgag cgccgccacg agaggcgggt gaagaatgtc
    10501 aatgcgacac cccttcctca ccaaactggt gctagttgct aagcgccgtt caacacgagt
    10561 tcccgatcgt tctatagcct cggcaagcgc atagcaaagc tgctcgaaaa ctgctgagtt
    10621 tttggtgggg tcaggattga gcaaaacttc gccaattgtc agtttggaat ccatcagcaa
    10681 ggtgacggca ccgcctatgt ggtcggcatc agagtgggaa agcatcatcg cctcaattcg
    10741 gttctcgcct gcgctggcga ggtgtcggga aatttgcgtt ccctttctgc cagtgtcgaa
    10801 aatgacggtc ccgccgtcgt catgcaagac ggcggcgttt ccgtggccca catcaaggac
    10861 gaataaacag ggttttggct ttcttgagga catcggcagc aggcagctca aatttgtcga
    10921 aatagagatc ttcttggcgc gcggccccga tattaacctc ggcaatcagc atgcttccgg
    10981 gcttaacgac cgcttgtagt ttcttgggaa ccaaatccag agggaatcct acttcctgag
    11041 cagagtccca ctggctcatc atgactttga cgaccttgga ggatcgcagc gtctcaatac
    11101 gctgcactgt catgattgtg cgataaggaa tgcgctcctg tggcgggtta ttctcgcgaa
    11161 cttcccggtc ggcacttagt aaagctgact ccaagtgtgt gcgatccaaa tcttccggtg
    11221 aacgaacgag agccggaatg cggcgcctga atagacgaag tgatgattca gcgtctgtat
    11281 tttcaaatgc tgtaaccaag acgcccgcga ttcctagcct gtaacactca gcgacagcct
    11341 gagcgccaag gaagggtgcg taatcccgct gttgtgacaa gtggtgatca caaaccacca
    11401 tttgaatgct ctggtgttcc aagaatgtct tcagaccgtt gagccttggc gttgggccaa
    11461 ttacggggaa agctgtggct ccgcagcgtg tcaccatgtc cgacaaccgc tctcggttgc
    11521 gagggttgtc ttcaaaaatg gcgatgcgcc ggttagcgag tgcggttgag ggcatgttag
    11581 cttttgagtg gaatggtgat ggtgaaagag gccccgccta attcgccatg tgcttcggct
    11641 tcaatatgtc cacgaagttc ctcaatcacg tcttttacga tggtgagtcc aagacctgtg
    11701 cccttctccg tggtggtttt tccaggtagc cagatttctt ccaacgccag cttctcaatg
    11761 cccggaccat tgtcttgaac gcgaatgatg gccatatcac cactccgccg ggtttggacg
    11821 cgaattcgcc gcacaccagc gttcgctgct aatttggcgc gggcttcgaa tgcctgtaga
    11881 gcattggtaa gaaggttcgt gaaaatcgcc tcgaacgctg cacgagggca ccagactcgc
    11941 agttcttcgt tcgcatagtc acgttcgagc gatgtttcgc gagcttcgaa atatggggag
    12001 aggagctcca ccgtttcatc aatgagttgg tggattgaat gccgtgccga gcgccgcttc
    12061 tcgtgctcaa gaagtctgag tgtgacgttc gagaacgagt agatggcatt cgcttcgagc
    12121 aggattcttt tgaccgcccg actgctgtcc tcccggaaaa gcagcagttc atcagggttt
    12181 cccaaataat tgtgcaagtc cttcgcgtca gcgacaatgc cacgtagcgg ctgttttgtt
    12241 tgatgggcga acgccgcagc cgtggtgcct accgttccga gtgtgaagta aagctgcgcg
    12301 gtctcgtcct tgattttcat ctctgcctcg tgagcagaac gaacattttc ggcggctttt
    12361 ttgaccttct gcttctcttt aggcgggagt gaagcaatca cctcatccat ttctttctcc
    12421 gccgcatttt tgtgctgccg cactgcttca gcctcggctt gaagtcggag cttccgcgcc
    12481 ttttcacgtt cacctgacat ccatgaaagc gcattgtcag caaatgttct tagtgtatct
    12541 agcgcatcgc cttctatgaa gcccaggcgg tcagtttttt gctggaaaat gcctgatggg
    12601 tcttcgattc ggatacaccc tgatgagttg tttgtgctgg gacgtaattc ttggtgctga
    12661 gaacgcttca gattcatatc cagccagtcg ttcttctgct ctccgtaagg catgacgcgc
    12721 acgcctcgat ggtaaagacg cactcccccg aattgtgcca accatgcttt caggccctgg
    12781 attgttgttt gctgactggc gacagcgaat cctttcttgg agaggttgaa ctcccataat
    12841 tggaacgaca gtgctggaat tttgtatgct ggcgcgtcct ttttgtcggc gatgtcatca
    12901 tgcttgccga caaacagttt tttcccacca gctccgagca cctctgcaga tgcacgccct
    12961 tgctgatcaa ttttggcaat gaggtggaag tcggattctg agaaataccc ttcttgggcc
    13021 agtgtctcta atgccgtgaa ctccttcgct tttaggacgg cttgaaagcc gcgttctttg
    13081 gcgaacgggt cacgtagcaa cagaatcgcg cgcgccaatc gcttgatgtc tgactcgcgc
    13141 catggcgaag gcaattgtga aatagtgatt tcagttccgt gactgctatg cggcaccttt
    13201 tcgcttgtga cggcaagttc cacatcttcc accacccgcg cgtgctcgaa cgccgcccaa
    13261 tcaatttcca tccggtattg ggtgcccggc tcagatttcg ggcgtgtggt gagttccacc
    13321 ttcctgccga ggcgaagcgc gccgagccgc cccaagcctt tgctgccgac cagcagccga
    13381 cctgatgggc tggtctcact gcggcttttc atcgagtcac cgacgatgag ccagccatta
    13441 cgaatctcct cggcagtcat tccaacaccg tcatccttga ggacaatcga accgcccttg
    13501 atgtgaacgc ctttcaactc gacggaacaa tgtttggcgt ccgcatcata ggagttcttg
    13561 atgagttcaa gaatgccttg gtcgggtgag ggattgagtt cttcccccag cagacgcagc
    13621 actgccgtag agaagcgaag tctctccgaa gttggctcct gagctggagg tgtctgaggc
    13681 atgatcaaaa ctcgacaggt atctctttta ccgcgccaac agtgaggtgt cggcaacgaa
    13741 tgcgttcatc aaggaattcg ttcacacgtt cggtcttgag ttgcgccatg agcgcacggc
    13801 aagtgtcggc ggacttgtca cgcggctcgc agacgatgag gtggttttcc acggcgacgg
    13861 gagctttgcc gaggatgact gtcgcggctg cacgatacgg atggccgggg cgcgaggtcc
    13921 gccgcagaac gacaaacggg ggctggtatg ccagaggatg atgacgcttg tcctcaatgc
    13981 gtctcacgtc ggtccatacg ggaatactac gcgggtgaat gtagcggcgg agcggcccgg
    14041 tttctttatc gcggtgaggg acgactcgac cgacatgaac atcaaagaag tcagcgatgg
    14101 ttttggtgtc agccgattcg acagcgacgg gccagcgatg cgagtcactc ttcttccctg
    14161 agcggcggca gacatccagg aggaaaacat ccacgtcggc gatgtcgtca aaaatgccgt
    14221 agcgttcgac tttgagaatt ttcgccaagt cacccacccg ttcacgccag cgatgctgga
    14281 aagaacccga gcgcagcacg tctggcaaaa tggcaagcag acgggtgcct gcgctggcct
    14341 gctccagcaa acggacgatg aaaatggccg cctccgaaac ccgcccgcca gcccaggcac
    14401 aatccgcagg cgcaggcaca agtccatagg gcggattcat caacaggtgt gtggcgcgac
    14461 ccaagagctg cttttgctca aggccattgg cacagcgaat atgaggaaag aatgatgaca
    14521 gatcatcagg cagcttctgg tcgccgccgt gactctgccg ggcaagcaaa gcaagccgga
    14581 gccgggcggc ctcaatgaac tccggctgta gatcggtgcc cgcaaggcat tggccccatc
    14641 gacgcaatgt ctcccgcaac gattttccca ggggcaggcg acgggcggca gccagcaaca
    14701 gatcagcagc tccaactgtc ggatcataaa tgaaagcgtt tttgccgaac gacagcgaac
    14761 cgacaagcct ctctgccagt gtcgatcctg tgaagaaggc tccagcagca cgtctcgccg
    14821 tcaaatcaac aagccgctgg agttctttgc cagccacacc atccagggag gcgtccacct
    14881 gcttgttcca cccagcatcg acacgcggag ccagcgccag agcatgcaac tctgcgctga
    14941 actggcggta acccgagaag tcatgaacca cagcctttgg catcctttca gcctatactg
    15001 gctgcctcgg atggaaaatt ctttcttctg ttccaggcca gaccaaacga tgtgattggt
    15061 gccctgccat actagccagc aaacagggga acgtggagat cgtgtttttg gcaagttggc
    15121 tcattgtccg ttttgtcaaa acgcccgctt gaatccatcg gatatgggcg ggaaatgaat
    15181 cggaagcccc aatgaaacct aagagcctgt ccggcaaatg gtgacaaaag ttagaaaagc
    15241 ggtgatgggt aggaatggcg aagaactacc ccaccgatgt cagtgacgaa gagtgggcac
    15301 tcatcaagct cgagatatgt gcgctggaca agccccgagg caaaggacgc atcgccagcg
    15361 aagcctcttc gcgagcctgc ttcaatgcct tgaggtatct gctcaagaca ggctgccagt
    15421 ggcggatgct gcccaaggaa tatccgccgc gctccaccgt gcatgacgcc ttgagtcgtt
    15481 ggacgcgcaa cggtgtgttg gagcgcatca acggtgagca gacccggcag cggcgcgagc
    15541 tggtaaaaaa aacgcccagc ccagcgcggc ggtcctcgac agccaaagcg tgaagtgcgc
    15601 cgacacggtt ggcactggcg aaggaggcag cggctatgat gcgggcaaaa aaatcaaagg
    15661 gcgcaagcga cacatcctca ctgacacgca aggactgctg ctgggcgtca ccgtcacacc
    15721 ggccagcgtg caggatcgcg acggggccaa agtcgtgtgc agtgtgtttt gccatcaatg
    15781 gcagcgcctg gaggtcattt tcgccgacgg cggctatgcg gggaagcttg aagggttcat
    15841 ccagcaggcg gcggcgggct ggggtcatgc acaaggattg cgcctggaga tcatcaagcg
    15901 cagcgaagag gccaaggggt ttgtcctgct ggcgaaacgc tgggtggttg agcgcacctt
    15961 tggctggctg atgaagtgtc gccgactcaa ccgtgatcaa gagcgtgacg cgcgccatca
    16021 cgaatccttg atttatctgg cgatgatcag cttgaacctt cgcatcctct caaaagcatg
    16081 atgcctctat tatttgccgg acaggctcta agcgcccagc aaattcggat actgcctcgt
    16141 tgattccgac gtccgcttac gccccattgc tcgccgattt gaaagcccgc gttcgagctg
    16201 cgcaggtgaa ggcggcggtg tcagtgaacc gggagttgat cctgctctac tggcacatcg
    16261 ggcgggagat tctgcgggcg caaaaggcgg agggctgggg cgcgaaggtg gtcgaacggt
    16321 gtttgaggtg aaaaagcggc tcattcaccg catcatgcgc ggagaatatt gcctttgcgg
    16381 tgaaagcctg ccataatccc cgcaataaga gcggagatta tgacatacat ccacgaatgg
    16441 gccgattggc ccaaactcac ctgggacgct gcgaagctgt cagctctgct ggcggatgtg
    16501 cggcatcgcc aggggcgttt gctgggcagg atggagggct tggggttccg cctccggtct
    16561 gaggcgcagc tttcgacgct gacggctgat gtggtgaaat ccagcgcgat tgagggggaa
    16621 aaactggatg cggaggaagt gcgctcatcc atcgctcgtc gcttgggatt ggaatacgca
    16681 ggtgccgccg tggcgagccg cgatgtggag ggcatcgtgg agatgatgct ggatgcgacc
    16741 cagaaccacg cgcagccgct cacggcagaa cgactgtttg gctggcacgc ggcattgttc
    16801 ctgaccggga gaagcggcat gcacaaaatc acggtggggg catggcgtcc ggctgaggct
    16861 ggggcgatgc gcgtggtgtc ggggccgata gggcgcgaga cagtccattt tgaagcgccc
    16921 tcggctgaaa agctggatca agagatgagg gcgttcttgg attggttcga ggcagccaac
    16981 ggagtcgatc ccgtggtgaa ggcgggagtg gcgcatttct ggtttgtgac gatccatccg
    17041 tttgaggatg gcaacgggcg catcggacgt gcgattactg acctcgaact ggcgagggcc
    17101 gatggaacgg cagaacggtt ctacagcatg tcctcacaga tcgagaccga gaggaaggaa
    17161 tactatcttc aactggagcg tggccaacgg aacggcctcg acgtgacggt ttggctggag
    17221 tggttcctcg gctgtctggg ccgcgccatc gcgaaggccg atgaaacact ctcgggagtt
    17281 ttgcacaaag cccgcctgtg ggagaaggtg agccaacaga cggtgaatga acggcagcgc
    17341 aaagtcatca acaagctgct ggatggattt gaagggaagc tgacctcctc aaaatatgcg
    17401 aagctggtga agtgctcgga agacacggcg ctacgcgata ttcgtgcctt ggtggagcgt
    17461 ggtgttttga tcaagaatga agcgggaggt cgcagcacca gctacacgct ggcgaccgtg
    17521 gcatgtgagt gaagaagccg cctatcaaac catgaatccc ttcgttcatc tcgcctgctg
    17581 tctcggtgct ttgatgctaa ctgcgccttt gctctcgcag gaagccgcgc tcaagcgccc
    17641 gcctgctcct gtattgaaga aaggagaaaa gcagatcttc gtggacagcg tgatgattcg
    17701 cagcaagcag ggcgtcacgc gtgtgatcca cccggcgaag aaactggagc gcccggtgct
    17761 cacggctgaa atgccatggg aagtgaggac agaagacggc atcatcgaca agcgggtgaa
    17821 catttacggc acggtgctgc gtgatgaaaa gtcaggatcc ttccgaatgt ggtatgccga
    17881 tcctggcagc gtgctttacg ccacctccag cgacggcatt cactgggagc gccctgtttt
    17941 gaatgtgaag ggtgcgacga atgaaacgga tctgcgtgtg cacagtccca gtatcatcga
    18001 ggacaagttc gagacggacc cggcgaagcg ctacaaggcg gtcggcaatg cgagcaccgg
    18061 agtggacgat gccaagctgc agcggctgaa ggacaagttt gagatggtgg attggtatcg
    18121 ggacaaaacg cagcgcttgt attacgcggc tcattccgcc gatggcctgc actggaagat
    18181 ggatcccgag ccgatcctgc tcggctgcga caccatcacg ctgtcgcagg acccggtgac
    18241 cggcgaatac ctcgccttac acaagcgcca gggcgacccg cgagtgatcg gcatccgcca
    18301 catttttctc tcggtcagca aggacatgaa gcactggtcc gagccgcatc cggtgcatgt
    18361 ggctgatgag gtggacaaca aagccacgcg caagctgaag ggcggcacct attcggaata
    18421 ctacaacctc tccgcgtttc cttatgcggg tcagtggctg ggcttcgtca ctcatttccg
    18481 acgcgtcgag ccgccgtccg ccttgttcgg caatgacgag gtgaatggcc gcaaaagatc
    18541 cgccaccggc atcatcgatg tgcagctcgt caccagccgc gatggccggc attggcaccg
    18601 atgctcggat cgcagtccag tgatcccgct gggccctcat gcctatgatg ccggcagcat
    18661 ctttggcctg tgcaacgcgc ccgttatcgt cggcgatgag atgtggatgt attacaccgc
    18721 ctcgaccacg ccgcacgggg gtctggcacc ggaaaaagag cagtccatcg cccgtgccac
    18781 ctggcggatt gatggcttgg cttcgttgca ggccaaaaac aaaccgggca tcatcgaaac
    18841 gcatgagttt gtccctgaag gcagcaagct cacggtcaat gcggatgtca aaagcggcag
    18901 catcgccgtg gaggtgctgg atgccgccgg acaagtcatc ccaggctacg aaaaggaagc
    18961 ctgcatgatc gaaaagcggg attcggtgaa gctggccatc cgctggaagg gcacagacgc
    19021 gctccccgca ggcaagcccg ttcgtctccg cttccatctg ctgaatggag atctgttcag
    19081 ctactggatc gattgaccga ccagcaaatc ccgcgccgcc tcatgaaact gcttcttctc
    19141 ctctcctgcc tgcttctcgc tccgatgctg attcatgcgc aggcgaaacc gcgcgcgcag
    19201 gactatcgca tcgtgtttca caatccggac cccgaacgct atgtggaagg acccgggctc
    19261 gtgcggctcg atgatggatc gctgctcgcg gtggtgcctg tggtgccgcg agaggaatgg
    19321 agcaaggagc ggcgtgccac gcagagtcgc acgcacattt tgcgcagcag tgatggcgga
    19381 cagagctggg cgcaggtgtc ggaactgccg tattactccg ccgctccgtg gattcaccaa
    19441 ggcgagctgt ttctcttcgc aaacaaaggc ggcacgaagg tgcgcaatga cgatctgctg
    19501 cttttgaaga gcaccgatgg tggcaaaacg tggtcggagc cggtgacgtt gtttgaaggg
    19561 cacttctgga actgccacac gggcatggtg caaaaggcgg accggcttta ctgggccacg
    19621 gatgatctcg cgcttggcaa actgcgcggg ccgcgcgtca tcgcgggcga tttgagcggc
    19681 gacccgatga atccgaaggc gtggcgcatg tccgagcccg tgccgtttct tggtgtgccg
    19741 gaggcgatga cgaatccgca gttcgacaag ctcagcagcc agtatctgga gccgaacgtg
    19801 atcgaagtgc gtggcaagct gcgcgtgatg atgaccgtga agccgaaacg ccaatccacc
    19861 gccggactgg ccgccgtgct ggactttgag gacaagggcg gaccgatcga cctgaagttc
    19921 acgcagtttc acccgatgcc cggcgggcag ttgaagttct gcatcatcta cgatgagaaa
    19981 tcgaagctgt tctgggcgac ggcgaacgcc gttgtggacg gccagggcgc gtatgactgg
    20041 ttccgcgagg gcgaaaagcg cggcaacgtg cgctacgcca gtggcctcgg cggtaatgac
    20101 cggcgtttcc tcatgctgca atacagcgtc gatggcttga actggttcca ggccggctgc
    20161 gtggcgcagg caccgaagat ctcgcaatcc ttcatgtatg cgcggcccgt cattgacggc
    20221 gacgacctcg cgatcatcgc ccgcagcagc atcaacgcgc caaaccaaca cgatgccgat
    20281 cacgccactt tccatcgcgt gaataacttc cgcagcctcg cactgaaact cacgccggag
    20341 cccgaggagt agctgcgatt ggcaaacgaa gtcgaacaac cacacaccga actcattcac
    20401 catgaagctc catcttcttt tttgtgtctt cgtcttcagc gctctcaatg ccgccgaacc
    20461 tcttctggaa aggaccgccg tttttccgcc cggcttcaac ggcatcgcgc gctatcgcat
    20521 ccccggcatc gccgtcacga ccaagggcac ggtgctcgcc tattgcgagg cgcgcaaaaa
    20581 cagcagctcc gactggggcg agatcgaggt gcatctgcgc cgctcgaccg atggcgggaa
    20641 gacctggcag gagccgaagc acatcgcgca cggcggcacg cgcatcgagg gcaatcctca
    20701 caagaaagac gacgcaggcg gacgcgaaca gaccgtgaac aatcctgtcg ccgtcgtcga
    20761 tcacgagacg ggggccatcg aaatgctcta ctgcatcaac tacgcccgct gcttctccat
    20821 gcgcagcacg gacgacggtg tcacatggag cgcgccggtg gagatcacgg ggacgtttga
    20881 gccgtttcgg aagcactatg actggaaagt catcgccacc ggtcccggcc acggcattca
    20941 gatcaaaagc ggccggctcg tagtgccgat ctggctggct tacggcaagg aaggcgatca
    21001 caaaccctcc gccgccgcca cgatctacag tgatgatcac ggcaagacgt ggaaggcggg
    21061 cgatctttgc ctgccaaacg aaggcgaact cggcaatccg aatgaaacca tgatcacgac
    21121 gctgtccgat ggccgcgtga tgctcgtggc gcgcagcgtt tcgcaggcaa gccgcaagat
    21181 catcaccacc agcgcggacg gagccacggg ctggacaaag ccggtgtttc acgaccagct
    21241 ctgggaaccg atctgcatgg ccagcatcgt cgcgcatcca tcgaagccgg gcatgctgct
    21301 tttttccaat ccgcacacgc tcggcctcaa caaggcaggc aaacccgtcc cagcaggccg
    21361 tggcaagcgc gagaatctct gcatcaagct cagtcgcgac gacggcaaaa cctggcccgt
    21421 gaacaagacg ctcgattccg gcaaagccgc ctactcagac ctcgccgtgc tgccggatgg
    21481 cacggtgctc tgtttgtatg aagccgacac ctccatcgac tgcgcacgct tcaatctgga
    21541 gtgggtcacg cagtagcttc catcagcggt cgaggccgaa cttctccaag cggctcgatg
    21601 gccggtcgag tccatctgct ctcgtcagag ttgaaggctg gcttctttgc aaagactcga
    21661 ttgaatggta ttccccgaaa tgaatactcc ccttcgccat cctgatctca agctcggcac
    21721 ctggctgcag accggctcct ccgtcatcgc cgaactggcc gatttgagcg gctttgactg
    21781 gctgctcatc gacctcgagc acggctgcgg cacggaggcg gcggtgctgc cgcaaatcca
    21841 ggtcatccgt catgccgccg ccatcgtgcg cgtcggtgcg ccgcaccctg agcagatcgc
    21901 gcgggcactg gactgggggg cggcaggcat catggtgccg atggtgtcca cggcggaaaa
    21961 ggcggaggcc tgcgtccgtg ccatgcgcta tccgccgctg ggagaccggg ggctggccgg
    22021 catggtgcgt gcctttgatt tcggacttca tgccgaaaag cgcactcccg ttttccatgc
    22081 ccagatcgag accattgagg ccgtggaaaa cgcgcgcgcc atcgccgccg tggatggcgt
    22141 ggacgtgctc ttcatcggtc cgatggattt gaagctcaac ctccagtccc gcccgggccg
    22201 cactagtttg gatttcgccg cctgcctgcg agaggtagcc gctgcggcca aagcggctgg
    22261 caaggcctgc ggtctgctgt gtcgccagac cgacgacttt gccgagttgc aggccctcgg
    22321 tttcacgcat cttgccatcg aaacggacat caccctcctg cgcgagagct accgcaacgt
    22381 cgtccaacct cttcgggtat gaatatgaca ttgagaacca cctatgccct gttagccaca
    22441 ttctgtgtct ccgcgcttca tgcggatgat tccaagccgg tgctgcaact ggattttggg
    22501 caggaggagt cggtgccttt gattcctgtc ggccatgtgg tgcgtgatca ggcagggccg
    22561 aggccgccgg attttccgga ttttgagccg aacaacacgg cgattcagtt gaatggaaag
    22621 ggggcgcgtt ttgaaatcaa ggacacgggg ccgcagagcc ggttcgattt cacgaagggc
    22681 gatgccatca cgctggaatc ctgggtgaag gtggatcaaa tcgggcccgg gcagccggtg
    22741 tatatcatcg ggaaagggcg caccaactcg ccgcactttg cccgcaacaa tcagaactgg
    22801 tcgctgcggg tgatcggcgg cagcgcgggg ctggcgcatc tgagttttct cttcgccggt
    22861 gcgccggaag ccgggggcgg aaacacctgg cacatctgga actcggaggc gtcgttcaag
    22921 atcgcgaccg gctggcatca tgtggccctg gcgtatgagt ttggaaagcc ggactcgatg
    22981 cggggttgga ttgatggcgt ggcgacgacg ggcacttggg gcgtggacgg agccaccacg
    23041 aaagcaccgg tggtggatga tgacgatgtc tggatcggct cgtcgcaggg cgggaacgcg
    23101 gggaacagtc tggtcggctt tatcgattcc atcgcgattc atcgccgcat cctgaccgat
    23161 gctgaaatcg cgaaacactc ccggcggaag gatggtcctg aagtggtcct gcctgctgtg
    23221 gcagagatgc ctgatttggg aaaaatcgag cgcggaaccg tgctcgtgca gttcaacgaa
    23281 ggagccgctg catctcatcg ctggcccaat tcacaggaaa tgccgaaaga ggctgcgcgc
    23341 tggacgggcg atgcctttct gctgccgcgt gtgccggtgc gctacgatca ctggggcatc
    23401 cgctcgagct gggaagcgcc gcttttgctg cgcatggcgg cggatgtgga gttgcccgca
    23461 ggctcgcgtc gcgtgctgct gcggacccgc ggcctgtccc ggctctggat cgatggcaag
    23521 ttgatcgctg aaaccaaacc ggctgtcgcc aacggtgaaa atggcttcga cccgctgact
    23581 ccgttggcgc agccgccgca tccgggagtc cgcgtcagag gttaccatca gcaggaggtt
    23641 tttggcacgg tgacggtgcc tgccaagaag acgacctcgc gtgtggtgct ggaggtgatc
    23701 gtcggtggca aaaacatgcg cacggatacc ggcgaggtct gtgtcgcgct cgaatcagag
    23761 cgtggggatg cgttttccat cctccatgcg gccgacaagc cgggtcttgc tctgacagat
    23821 gccgaggtcg agccagcgct ggtcgcaatc gaagcctcgc tggcccgttt tgatgatgca
    23881 agccgtcttg cggcttcgcg gtctcgtgat gcgttttggc aaaagcgaca tgatgcggcc
    23941 aaacaatggg tgaagaagaa tccgccgccc aagccgccac atgaaggcca tcccgtggat
    24001 gcatttctcg atgcgaaaat cgagaaggcc cgcgcggcaa gcgccgccac cacggacgag
    24061 gcgaaggcat ttcacagcga ggttttgccc gttctgcggg agcagtgttt ccgctgccat
    24121 ggcgaaaaag aaaagggcgg actgaagctg aacacacgcg aggctgcgct gagcggcggg
    24181 gattcggaag ttcccgcgat cctgcccggt gatccggcgg cgagtgagct gatcaagcgc
    24241 ctccgcacca atgacgagga tttgatcatg ccgccgacag gcgaccgcct cagcggagag
    24301 aagatcgcgc ggctggagtc atggattcgc aacggtgccg catggccatc gccgcccgtg
    24361 gatgcctcca ggctcgccca aacgcccctc acctccgatg cgacatttct tcgccgcatt
    24421 tatctcgaca ccatcggcct gccgcctgct cccgatgagg ttagggcatt tctcgcggac
    24481 accgaccctg acaagcgccg caaggtcatc gacaggctgc tggccgatga acgctgggcg
    24541 gatcatgaga tgagcgtctg gctggacgcc ctcgcggaga accccacgct gatcaatgcc
    24601 tcgctcaaca gcactgggcc gttccgctgg tttctccacg atgcgttgcg cgaccacaaa
    24661 gcgctcgacc gcatggtcac ggaactgctc atgatgcgtg gagacgccgc ccacggaggc
    24721 agcgctggct ttgcccaggc ggcggaaaac gatgcgccct tcgcggcgaa ggggcacatc
    24781 gtggccgccg cctttctcgg gatcgagctc cagtgcgctc gctgtcatga ttcgccgtat
    24841 cactccacct tgcagcgtga tctctactca ctcgccgcga tgctggagcg caaggccgtg
    24901 acggtgccga aaaccagccg ggtgccagcg gccttttttg aaaagaaggc ccgcgagtcc
    24961 ctgatccgcg tgacgctgaa accggacgaa ctcgtgacgc ccgtgtggcc cttcgccagc
    25021 gtgaccggtg tggcggaagg ccaggctctc gacacactca tcgaaaatcc gcaggacaca
    25081 cgcgaacggc tcgccgccct cgtcaccgcg ccggagaatc gtcgctttcc ccgcgtgatg
    25141 gtgaaccgga tttggaagcg gctcatgggc gcgggttttg tggagccggt gcaggattgg
    25201 gaagggcatg acgccagcca tcctgagttg ctcgactggc tcgctgccga actgatttcc
    25261 agcggctacg atgtccggca catcatccgt ctcatcgtga catcgcaggc gtatcagcgc
    25321 gaggcgggtg cggaaaaatg ggtgtctgtc gccgctgaag agcgtttctt cagcgcaccg
    25381 gcacgccgcc gtctcaccgc cgagcaggtc gtggactcgc tccacaccgc tgcgggagct
    25441 gtcatcgatt ccgaaccaat gaccttcatt cacgacggct ccaaaacgct gcaaacacgg
    25501 caggatctgg ggcgtccgcg tcgtgcctgg atgtttgcga gcctcaacaa cgaacgcgat
    25561 cgtcccagcc tcgctctgcc gcgagcgcag gccacggtga atgtgctgga ggcctttgga
    25621 tggagtggct cccgtcaaaa accgatcttt gatcgcgaca ccgatcccaa catgctccag
    25681 cccggcatcc tcgcgaacgg cacgctgacg cagtcgctct cacgggcagc gtggaagtct
    25741 gaactggccg atctcgcggt tgaggcggca tcaccggagg ccttgctaga ggagctgttc
    25801 ctccgctttc ttagccgcat gccgttgcgg gcggagcgag agtcgcttct cccggcattg
    25861 caggctggtt ttgacacgag actcacgccc gcaggtcaaa tcgtggagcc tgcgccgctg
    25921 ccacccttgc gtctcgtgac ctggctgaac catgtgacat cagatgccaa caccatccag
    25981 caggagaacg aaaaccgcgc gcagcgcggc cccagccccg atccgcgact gcgggcggaa
    26041 tggagggaag tctatgagga catcatctgg agcctgatca acgaccgcga ctttgtctgg
    26101 gtgccgtgac gcactcgcat catcaacaca ccaccactca tcatcccatg aaacggagac
    26161 acttcctcaa aaccagcgcc accttgggcg gcggccttgc tttgccacac ttgctgttcg
    26221 cggagtccag cgcgcagccc attcgcggca aagcagagca cgtcatttcc atctggctgg
    26281 gcggcggcat gggtcagacg gacacttttg accccaagcg cctgggcgac ccgaaagcca
    26341 agaaggctgg ctcctactac ccgtccattg agacggcggt gcccggcgtg cgtgtgtgcg
    26401 agcatctgtc caaaatggcc ccgctcatgg atcgcgtcac ggcagtgcgc acggtgaacc
    26461 acaacgtcat cgacgagcac gccgctgcca ccaatcgcat gcacaccggg cgtcccatca
    26521 gcggcaccat cagctatccg tccatcggct ccatcatcgc caatgagcgc ggagccgtga
    26581 ccgaagatgc cccgccctat gtcctgatcg gttatccgaa cgtcacacgc ggcccgggat
    26641 tcctcggcgc tcaatccagc tatctctatc tcaccgacac cagtcgcggt ccggcgggtt
    26701 tgtcgctgcc tccggggatc tcagtgcagc gccagtcacg tcgcgagcgc ttcctcgcct
    26761 cccttcaggc gagcgccgga gagacccagg acaagcagtt gaaaagctat gatgacgtca
    26821 tcgcgcaaag cctgaaactg agcgggccgg agttcaatcg cgccttccag ctcgacaccg
    26881 aaccggcgga cttgcggaat caatacggcg gcgagtttgg acagcgctgc ctgctcagcc
    26941 gccgactggt cgaacgcggc gtgcgcttca tcgaagtctc gcacaatctg aacttcatca
    27001 atggcgccgg ctgggacacg cacaaggagg ccatcctcca gcagcatgtg ttgatcaagg
    27061 aactcgacac cgccgtggcc gcactgatcc gtgatctgga ggcgaagaag ctcctcgaca
    27121 agacgctcat tgtcatcacc accgagtttg gccgcccacc ggagttcgac agcggcggcg
    27181 ggcggggcca tcaaggcagc gctttctcct gcgtgctggc cggaggtggc ctgaaacact
    27241 gcggtgcctg gggcgagacc gacgagctct ccaaagagat cgtcgccaac cccgtcagcg
    27301 tgcccgattt cttcgccacc atctgcgccg ccgtgggtgt cgattaccgc aagaacctct
    27361 acgacggcga ccgccccgtg cccgtcacgg atcaaggaag cccgattgcg gcgttgtttg
    27421 gatgagtcga atggctgcaa caagatcatg cgacttaact cccccatatc cattccaggc
    27481 ccaaaccggc ccggcactct ttgcacttcc attcactgcc aaaccaaaca gcactgctcc
    27541 ctggctggca acaactgcag gcatcagcct ggcctcggtt tcatgtggcc aagcgctgcc
    27601 acttagatgt ggaaatggga tctgcatgtc tcccataccg tgtatatggc gaagccagga
    27661 gttgtaagtt cagcatgttg ctcgtccatg cctacttctt cccgctgctg atgaactcct
    27721 cacgcgtgac gaagccatcc ttgtcggcat ccagcttcac gaagcgggct ttggcctcgg
    27781 ctttgtcgcc gccttggccg gcgaggtatc cggtttcgtt gagcttcggt ttgccggggt
    27841 agaggcggtc gtagcgtttg tcgcgcggct cgttcgggtc gtaggctttg cttttggagg
    27901 cgccgacggt cttggcgacc ttgccgctct tctttggagc aaaatcgctg cccacaggcg
    27961 gcagatgttt tttcagcgat tcgatcaaag ccgcgtgctc gggcttggtg gcgaggttca
    28021 cggtctcgtt cgggtcgttt tgctcgtcgt agagctcggt gaagccgatg tcggcggcgt
    28081 tgcgcttgcg ccagaaggtg cagcgccatt gctcggtgcg gatgctgtag cccatgaggc
    28141 tgccgccgtg atcctttgac gaacggggat aaatactgat cgcgggcttc tgcatcggcg
    28201 cgtcaggatt ttcgaggtag ggcttcaaac tcatacccgc gaggccctgc ggcaccgtga
    28261 ggccacaaac atcggcgagc gtgggataca catcgacgaa ctcgaccggc gcggcgcagt
    28321 gtttgcccgc ggtgctgctt ttcggcaagg cgatgagcaa cggcgaacgc gcggcgatct
    28381 cgaagttcgt gtgcttgtgc cacaggccgt gctcgccgag ctgccagccg tggtcgcccc
    28441 acaaaacgat gaccgtgttg tcggcgagac cttctttgtc aagcgcatcg agcacacggc
    28501 cgatctgcgc gtcggtgaag ctgatgcagg cgttgtagcc gtgtcgtagc gtcttcgcga
    28561 aatcggcggg gaggggattt cctgctggca cgccaggata cgcgtgcagt tcgccattcg
    28621 tgtgaccgac gaaatcaggc gtgccttccg gatacttctc gaaagcagga ccggggatcg
    28681 tctccggatc gtgcatgtcc caatacttct tcggcgcgac aaaaggcaga tgcggcttca
    28741 caaagcccac gccgaagaaa aacggctttc cggccgcttt gagtgccgcg agccgtttca
    28801 cggcctccgc agccaccgcg ccatcgggca gctcgtcgtc gcttttcggg ctcacctcat
    28861 aagaagggcc ttttttgccc gctttgccgc ctttgcgctt cgcgacgtct tcgagcggat
    28921 tcgcgaactc gctcggcacg ccttcaaatt tcgtcaccgt gtgcttcgtc cagtctttct
    28981 cgtccacttt gaccatctcg ccgctcggga accaccgcgg ctcggtccac gacgggccgt
    29041 cttcaaagcc atgatgctcg atcttgccga gcgcggaggt gtgatagccg ttctgacgaa
    29101 aatgctgcgg cagcgtcacg caatccggct gcgcatcacg aaagtgcgtg ttgaggtccc
    29161 acaccttcgt cgtatccggc cgcaaccccg tcaaaatcgc cgtgcgcgaa gggctacaca
    29221 ccgcctgcgc acagtaagcc ttgtcgaaaa cgatgccccg agccgcgagg cggtcgatgt
    29281 tcggcgtctt cgcatgcttc acgccatagc agccaatgtc cggccgcagg tcatcgaccg
    29341 cgatgaagag cacgttcatc ggctccgccg aaaaagcgga gagcaaaggg caaagggcgg
    29401 agagtaagat gaggaagcgc atggtggtgt gcatggataa ctgccggtgc gggcatagtt
    29461 tgccgggaaa gtttggttga gaggacttgc ccatgcgttt gagtcttgct cccatgaaac
    29521 gccgcgtctt tgtcgcctct cttgccgccg ccagtcttcc ttcgcgggcc tcggcggtgc
    29581 cggagctggc gggggaaatc ggcatcacga ccggttcatt catgcgtcat ctcagtgcgg
    29641 atgcggctcc gggaaagctg cgcctgctgg accttcctcg acgaatgagc ggcgagctcg
    29701 gcctgcgggt gatcgatctc atgacggcca cgctggcgga gctgacgccc gcctactgcg
    29761 aaaaactccg cgcggaggcc gggcgatgcg gctgtgtttt gaccaatctc aaaatgaatc
    29821 aaaagggcct cgacctcggc agtcccgaca aggctgtccg cgatgaggcg ctgcgtgttt
    29881 acaaagagac catcgacctc gccgccatcc tcggtgtgcg ctgggtgcga ccgctgccga
    29941 tgctccaaag gccggacctc tcggtttaca aggacagtta ccgcgccctc atcgactatg
    30001 ccaaagcgaa gggcatcggc gtgctcgtcg agaactttgg ctggatgcag ggcgatcctg
    30061 atgccatccc gcgcctcatc gcggtggtgg gcgacgattt gaaatcgcaa ccggacaccg
    30121 ggaactggac cggcaacgac gtgcgctacc ttggactcga aaaagccttt ccattcgccg
    30181 tgtcgtgcga tttcaaatcg cgagtgctcg gccccggcgg cagccacacg gagtatgact
    30241 tgaagcgctg ctttgaaatc ggctggaagt cgggatttcg cgggccgtgg tgctttgagc
    30301 atttccacca ggacttggag cagcagttca aagagtttgc cctcctccgc gacatgctgc
    30361 gcgggtggat gaacgcggcg gagtaagatt gcctcccgct gcgcgttgat gggggcatat
    30421 gatcgaccgc agaaatctcc tccgacagtt cgccgccacc gcaggtgcgg cgtttggcac
    30481 gtcgttgttt cctggcatcg cacaggcggc ggctcgcagc acgtccagcg gcgggccgaa
    30541 gcgcgtgatc ttcttcctgc aaaatcaggg ctttgatcca aagacctgca tccccgcagg
    30601 catgaaaaac agcggctcgc tggccagggc gaagctgccg gagcccatcg aggcgctgga
    30661 gcctttcaaa gagcgcctgc acatcatcaa tggcctgcac ggccttcata cgagtccgtc
    30721 gcacagcgcg ttcttcggtg cgctcggtgg ttatcgcggt gacgacggca cgccgccgct
    30781 cgctcccacc attgactatg aactgagcca gatcctgccc gagacgctgc tgccgcatct
    30841 gtgcatcggc atggactcca tcgaaaacat gcgggcgaag cccaccatcg ccaccttgtc
    30901 cgccagcggt gcgggcgagc cgatcttcat gcactcgaac ccgaatcacc tctaccagat
    30961 gctctacggc ggcatctcgc agggcgacat tcgcaagcag cacgaggcgc gctccggttt
    31021 gttcgaccgc atcgagcaaa tggcatccgc gaaaggcggt gcgctgccca ccggcgagca
    31081 gcagcgctac gggcagttcg tgcagggctt ccacgacatc aatggcctgc gtcagcgcct
    31141 cggcggcgtc tccgatcact tgcgccagtt cgcgccgaag gtggacgagc gctacacgaa
    31201 gccgaagttc gagaccgact ggcatgacgt gctgctcgac ctcggcatct ccgcgctgaa
    31261 gtccggcatc accagcgtgc tgaccatcgg ctccggtcgt ggcgagatct tcggcgcttg
    31321 gagcggcctc ggcatcaccg agcaagggca caatctcggc cacatggagc agccggacaa
    31381 tccgatctgg atcaagatcc gccaatacaa ctgccgcatg ctggtgaagc tcatggagca
    31441 gctcgaaagc gtgcccgaag gcagcggcac gatgatggac aacacgctca tcgtttacac
    31501 cagcaacaac gccgacaaac agcacaccag cggtgccacc tggcccgtga tgctgctcgg
    31561 caactgcaac ggcgtcttca aaaccggctg cttcacccag ctcgacggca cgcgcccgat
    31621 caatgcgctc tacgccacgc tcctcaaaac cgtcggcgtg gaccgcgacc gctacaacat
    31681 gagcggcaag atggccgcaa agttcgactc cggcaccggg ccactcaaag aacttctggc
    31741 gtgagtattc ttgcaacagt ctgtcgcaac tggcttggcc tgagtttctc agtttccaga
    31801 ggaaaaatcg ccatgccggc tggaaggcaa gccctatttc ccagcctctt ccagaagctc
    31861 gggcaatcca cgaagacgat agcgcttgca cagcgcaagg atctcgtcgc gatcagcgtc
    31921 ggtgttcctc cgcagcagct ccaccacgtc ggctttggac ttgaatcccc cggcgtagag
    31981 tttcagggcg acaagatgcg gcagtggcac cacgcgcaag gcgctctccg ggcggacggt
    32041 cagggggggc gcttggagcg cgtcctcaat aacggcagga aaggtctgac cgaagttgac
    32101 gagttggatc aagccaccag ggcacatcac atccatgacg ccggagagcg gatcgtccgc
    32161 gtccggctcg cgcagttcca catgaaaacc cctggcgcga agagcctcgg caatcgcgcg
    32221 aaacttttgc gcactcgcgt tcattccgag atcgaggtcc tccgtgaaac ggacatagtg
    32281 atgggctgca agagcgagcg caccgatgac cacagcctcc gtctgaaact ccttcagcac
    32341 ggccacgaca ctttcagccg catggagaat cgattcagga tgcgcattca tgcttcgact
    32401 atttcctcgc cgaaggctcc agccagccaa atttcgtctt caggccaagt gcagccagga
    32461 ttcgttcttc caccgtcgtc agccgcgagc gttccaactc cgcattcaaa gaagccttcg
    32521 cgcatgcgct ggcgcgaggt ttcagggact catggttttc tggcacggcg atacttttca
    32581 ttcagtcgct caaagggtag ccgctgtcgg agcggctatc aaggtttcat cgccagcatc
    32641 atggatacgc taccgtctct tctcttggcc gcagcgactc tcgtctgtgt atctgccctc
    32701 gccgccgaaa cctacacgcc cggcgagccg gtgcgcggag gcttcaagga cttcgcgcac
    32761 ggctttctcg agcagaactg cttcgagtgc cacgacgacg agaccacgaa gggcgatctc
    32821 aacctgctcg aactcggccc ggtggatgaa acgaacacgg ccacttggaa atccgtgtgg
    32881 gcgcaggtca cgctcggtga aatgccgccg aaaaagaaaa agtcacagcc gcagatcatc
    32941 gagcgcttgc gcttctcgga ctggatcgtc ggacagctcg ggcgcgtgat gaaggacaag
    33001 ggcggctttc atgagccgtc cgatcctcag aagggcaact tcctccgcca tgagctgctg
    33061 tttggtccat tgccggaagg catccgactc gtgcccacgt cttcgcccgc gcgactttgg
    33121 cgcgtgactc cgcaggagca catcacgcgg ctgaatgaac tcatcaacat cgagccgccg
    33181 tttgatccga agaagcccgg cctgcgcacg catggcgatg cggtgcccac gaatcatggc
    33241 ggtgagttga agctctactt cggcacggac aacatcctcc agtgggtggg cggcaccgtc
    33301 gcgtatgcga ccgcggtgaa gagcgtgccc gaagtgctct cctcgctgcg caacaccggc
    33361 ctggagaact acccggacct ctattcggtc aacagcgcgg aatcgacgca gatcctcggc
    33421 atggcggagg acatcctgcg cttcatggcc tacggaccac gcagcctcgt caccttcccg
    33481 gagcagatca ccgacgatcc gaagacctac gacaaagtga agccgaaggg cgacattcgc
    33541 ggcctgccga actccatcgt ctatagcacg aaggtgctcc gccccatgac tcccgtcgct
    33601 gacttgatga aggaagcgac cttgtccgat gaaaaactcc tcgcagcggt gaactggctt
    33661 tttgaagcgc tcacgtttcg tccgccgaca aaagaagaat ccggcaacta ccccgcgatt
    33721 gtcaaagact cgatccagaa ggtcggaaaa gaggaaggcg tgttcatggg gctgtcatcg
    33781 ctgtttctgg atcgcgatgc gcttttccgg cctgagctcg ttgaggctgg caaacccgac
    33841 gcgcatggcc gcgtgatgct gcaggactgg gagctgggcc tcgcgctgaa tcatgcgctg
    33901 tgtttcatca agcccgatgc cacgctgcgc tccgccatca ctgaagggcg catgaagacg
    33961 cgtgaggacg tgcggcgcga ggtcacgcgc atgctggatg acgaccgcat tcgcaagccg
    34021 cgcgtgctgc gcttcttccg cgaatacttc gatcacgaca tggccggcta cgtttgcaaa
    34081 gacgcccggg cgctcgcggc caccggcctc tcgaaccgcg cctccatcta ctaccacgac
    34141 atgttcgatg ccacggccag caccgaccac atgatcgaac tcgtcctcgc ggaggacaag
    34201 gatgtgctca aacaactgct caccacgcag aaggtcgtcg ccacgccgag agacaagttt
    34261 tacttcggca ggttcaacag cgcggaggag gtgaaggagg ccatcgaagc caagaaaaag
    34321 gccgacgagg cacaggaagc gaaggaaaac gcccagatag cagcgctgaa ggcggagatt
    34381 gccaaactga ccgctgcggt gaaagccgtg cctgctgaca agacggcccc gcgaacgctg
    34441 gcgctgaaga aaaagtcatt aacggccatg gaaactcgtt ttcaggaagc cagggccgca
    34501 cggaaacgcg gcgacggcga aaacatgaaa gtgacctacg ccgaatttac gggccccgag
    34561 acttttgcgc gggtcacacg gcggtcgttt ggcgatcaat cgctcacacc cgagcgcatc
    34621 ctcgccaccg cgcccgaagg ggagcgcctc ggcattctca cccatcccgc atggctcgtc
    34681 tcgcactcgg atgccatgga taaccacgcc atccatcgcg ggcgctggat tcgcgagcgc
    34741 ttgctcggcg ggggcatccc cgacgtgccc atcacggtgg atgcgatgct ccccgtcgag
    34801 ccggagagca cgctgcgcga gcggatgcgt gtcacccgcg aaacctactg ctggacctgc
    34861 caccagaaga tggacccact cggcctgcct tttgaaatgt ataaccacgt cggcttcttc
    34921 cgcaccaccg agctggacaa gcccgtcgtc accaggggcg agatcatcga cagcggcgat
    34981 tccgcgctcg acggccccgt caccaacgcg ctcgacatga tcgcgaaact cgcgcagagc
    35041 gagcgcgtgg agcaggtctt cgtgcgccac gccttccgct tctggatggg ccgcaacgaa
    35101 accctcaacg acgcccccgt cctccaagcc gcccaccgcg cctaccgcga caacggcggc
    35161 agcatgatgg ccctcatcac ctccctcgtc acctcggatg cgtttctcta tcgcaaggtg
    35221 gagcgcgctg cggagtgagc cgccacactc tcatcgccat gcccagccgt tcgttgcacg
    35281 ctctgctcat cgcgctcggc ctcgcactcc aaagtcacgc cgctcctccc aacgtcatca
    35341 tgctgctcgt ggatgacatg ggctatgcgg acctcggttg catgggtgcc aaggacatcc
    35401 gcacgcccca catcgaccgc atcgcggctg agggcgtgcg catgacagac ttttacgcga
    35461 acgctcctgt gtgctcgccg acacgcgcgg ccttcattac cgggcgttgg cagcaacgct
    35521 gcggcatcga gtgggccatc ggattgactt cggaagagga gaagcgcgtg aatggagcac
    35581 tcgtgcctgt gaaagaacgc ttcacgatgg gcctgatgcc cgaggacacg ttcctcgtgc
    35641 cgcttttgaa gaagaatggc tatggcaccg cctgctatgg caaatggcat ctcggcttca
    35701 aacccgagca caatcccgta aagcacggct tcgacgagtt ttggggcatc ctcggcggcc
    35761 agtgcgacta ctaccgctac cactacatcg acggcatgaa gcagctctac gagaagaccg
    35821 agccggccac cgccgagggc tacatcactg atctcatcaa ccagcgcacc gtccgctaca
    35881 tccgcgagca gtccaggcag ccctttttca tctacgtgcc ctacaacgcg gtgcatcatc
    35941 cttatcaggt gccggacgat cccacgcagt tctacacggc cgagaacaaa aacgacggca
    36001 cccgcgccgg ttacgccaag atgctcgaac gcgtcgatca aggcatcggc tacatctttg
    36061 ccgcgctgga ggaaaaaggc atcctcgaca acacgctcat catcttcacc agcgacaacg
    36121 gcggcgagcg cttctccgac aaccgcctgc tcttcaatca caagaccacg ctatgggaag
    36181 gtggcatccg cgtgccctgc ctgctgcgct ggcccgcagg cggcctgccc aaaggcgctg
    36241 tgtccaccca gaccgccatc acgatggacc tcaccgccac cctcctcgcc gccgtaggcc
    36301 tgcctgtgcc tcccacgatg gatggcatta atctgctccc catcctcagt ggaaaagcag
    36361 cacccataga gcgcacgctc tgctggcgcg tggaccgcac cggccgcaat atgaaagcca
    36421 tccgtcacgg cgagtggaag tatgtgcagg acgacctcgt cgagatgctc ttcaacctgc
    36481 gcgaagacat cagcgaacgt cacgacctct accacgagca cctcgacaag gtgaaggacc
    36541 tcaaagccaa actcgccgcc tgggaagccg aagtggacaa agtcccgccg cccattcgga
    36601 ttcattgatc tgctttgtcg ggttgcctca accggccggc gccatccaca gtgaccgctt
    36661 cgtcggttta acgtgaaggt gcggtgcgat ccgcaggact gactgcggtg agtccagagg
    36721 actctaccac cctacttttt cttcttcacc ttctccacca tgctcgcgcc gacttccttg
    36781 cgccattcgt tgagcttggt gaggaggcga tctcgatcag tggggcgctc gtcggcgagg
    36841 ttcttcgatt caccggggtc ggttgcgagg tcgtagagtt cgaggcgttt ttcgccgggg
    36901 aagtcgagga gcttcaaatc accgacacgg atggcggagg aaaacagatc gcccgtgctg
    36961 gcgggacgtg gcgcggggtt gtgccagtaa aaagtgtcgc gggcgggagc ggtgccgttt
    37021 tgcatcaggg gcacgaggtt ctgaccatcg gtgccagcga gattcggcag cgcgatgcct
    37081 gtcatcgccg cgagcgtggg catgaggtcg agtgtgctga cgggcgtggc gatctccgtg
    37141 ccgggtttga tgttgcccgg ccaacgaacg aggaagggaa tgcgcaggcc gccttcgtaa
    37201 agatggcctt tgccggcacg gagcgggcgg ttcgaggtgg cgacgccgcg attgttgccg
    37261 cgggcgctga gcccgccgtg atcgctggcg aggacgatga tggtgttgtc ctcgatgccg
    37321 agctttttga gctggccgag caggcggccg aggccgttgt ccacgctctc gatcatcgcg
    37381 gcgtaggtgg cgttgttttg cacgagctgg ttctcgcccg cgctctcttt ttcccattct
    37441 acggcggctt tgggcatggt ggcgagtttt tcctcgtagc gcttcgtgag gtgctttttg
    37501 gcctcgatgg gcgtgtgcac ggcgtagtgg gccagcaccg cgcagaaggg cttgtcctta
    37561 ctcgactcga tgaacttcag cgtctcctgc gtgaggcggt cggtgaggta ttcgccctca
    37621 ggcgcatcgt cgaggccgac gatggcgtcc ttttcgtcgt ggcctttcga cttcgatatg
    37681 gtgtagggcg cgaaaaacga gcgcgtagct ccggcagccc cggcggcgac gatggtatca
    37741 aagcccatct ggtctggatt cgacgcgcct tcgccgagat gccatttccc gcagtaaaag
    37801 gtgttgtagc ccgccgcttt gaacgcctgg ccgatggtgg catcgcgatc cggctcgaca
    37861 tgcgggctgt cattttcccc ctgcacgcgt gcgggatgct tcccggtgaa gatggcgaaa
    37921 cgtgaaggca cgcagcgcgg atacgccaca tacgcttgcg tgaactttgc cccgctcgcc
    37981 gccagccggt ccatgttcgg cgtttcgtaa agcgtggagc cgtagcagcc gagatcgcga
    38041 gagccgaagt cgtcgatgag gatgaagagg acgttcggct gggcggcgaa gagcgaagaa
    38101 ctcagggcga ggaggatggc tagaatgcgc atgacgacgg atcactgctc aagcagtgcc
    38161 cgtttgccaa agaatctccc gacgccatca acaactcaat actcgaccga cttgtcgagc
    38221 acgagttgct tcatcgcctc gccgggcttg agcttgtatt ggagctggcg tgtcttgccc
    38281 gtgggcgctc cgtcgccctg gataggaaag cggcggatga aggtgccttc gacgaactga
    38341 tactcatctc cgccgccata gccgttgatg agctttgggg tgtcggtgat gtcgcgcagg
    38401 ttcaccgcgc tcatggactt tttcgcgttg gtggagaaga cgtgggcggt gccgcgctcc
    38461 tgtggcccgt cctgggtgat gacggcgatt tccggcgagt catcgccatc gatgtcatcg
    38521 cacagcagat tcacgacaaa gcccttgatg ggtacggtca ccggttcgtt cgaggcggag
    38581 agaccggagg gagtgagggt aaagctgttg cccgattcct tggcgggcga ggtcaccttg
    38641 aagctcatct ttccatagct cgcctgctgg gagaaacctg ctgtgtcatc tacaggagcg
    38701 tcggtggtgg cgtccggcgg cgtggccgtg atcttgaaga ggatgtcgaa cttggcagtg
    38761 acacctttgc gagcggcatt gcggttcaag aacaccgaga tgctgtgatc accggttttg
    38821 tagagctgcc cggaatactt cttctcaggt gccatggcgg aggcaaagag cgcgtcatct
    38881 ccaggaccgc ggcccggaat gtagatattg aagtcagcgg agggcgtgct ggcttgcagg
    38941 ctaacgctga gatactggcc gtcgcgggcg ttcaacttgt aaagaatgaa ctcttcgccg
    39001 gtgatgctgc ccttgatcgc ggcgctggtt gctcccggtt tgaattcgac acggacgacc
    39061 ttgggctcgg cggctgtggt ttgcaggaca gaggcagtca ggagcaaggc tgcggagaga
    39121 atggctcggg aattcatgat ggcaggtggg tttaaggatg aatatcccga cgtgcatgag
    39181 acgccgggac agagatttta tggcagatgg cttgatggat cactcaccca ttccagttgc
    39241 ccaggctggg aagccgccac ggatttcata cgccatcaca cgaccttcct ggcggccggt
    39301 gcgcgtgtcg aacaactgca cggtcgcagg accgtggttg ccacgtgatt tcacaacgat
    39361 ctgctgttgc tcagagatga agcgggtgtt ttccacgttg ggcgatgcgg tatagcaagt
    39421 ggacacacgg cgtgatcctt ccatgatggt aacagcgccc tgccccacgg atacggtgta
    39481 aggctggcca taataaggtc gtgatccctg ggagttggcc tgggaagctt tttccagttc
    39541 agcaagcacg gcgggatcaa tgacacattg tgttagggtg agactggcca gcccaatggc
    39601 ggtgatggca aggatctgaa gcgtattgtt tctcatatgg attgtgtgtg ttggtatggg
    39661 tttgaggggg gatctatttc tttggagccc agggctgcga cgaaaacgcc tgcgtctctt
    39721 ctttgctgcc tggcttgtag aagtcgtgga gggaatcgtt ctggtagtag ctccagaagg
    39781 catcttcgag cgccttgctc gcgatggtga aatcaccgcg ctgtttccgc tcctcgagga
    39841 atctgcgtgt ggcatcgagc ggctcaggcc cgccctgttg gtagccgatg cggccgccgt
    39901 aggaggcctg cagggcgcgg ttgaccttcc aatgcttgat gaacgcgctg gcgatctgct
    39961 ccagcgcctg tttgtcctcg gctgtctgcg gcgttttgtc aggggcggtg tccgccagaa
    40021 actgctcgat gagcgccagc tctttctcca gcgcctgacg ttccgttttg ggcaggccag
    40081 cggcggccat ccggcgtttg agcacattct tcgcggcctg cttttcagcc cggtctgccg
    40141 ccatgccttc ctccatggcc tttcgatagg cggtgatctc ctcagccttc acggagatat
    40201 ccttctcttt ggcaaaagcc tccagcagcc ttcccagcac cacataacgc agttcctccg
    40261 cgtctcgggt gtgaatcgtc gtgcccagca cgctgccagc cacgggcagc ttcgggtcgc
    40321 cataagtggg ttcggcaccc agcgcgccgg acaggcaggc gaacatccac gcaatcccac
    40381 ccatccgaaa aaactgtctg cgagtgccga gcatgatctg ggtggttaaa tgaaaggggt
    40441 ttgtgcaatc agtcctcaaa gccgtaagcc catgacgctc cgctgctgcg gatcgcgtag
    40501 gccagcactt tgtcgcgtag cgtgcctgtc gcggtgtcaa aaagctccac ggtggcagga
    40561 ccgcgagcac cccgtgattt cacgatgatc tgggtatcgc cacgcaccag ccgccacgtc
    40621 tccacgttcg gcatcgccgt gcggaatgaa cagagaacct gcccatcatt cctacggatg
    40681 atcatggctc cttgggatgc ctccgccgaa atgcggccct gtgccgtgcc caccggaaat
    40741 cggtccggca gttgcgtgac atagccaggg gaggaagacg gccgctggtt ggaatagccg
    40801 ggccgctggg gcaattgtgt cgggcgaccg ccgggatcga cgggtacgat gcaactggca
    40861 agggacaaaa gagcggcggt ggtgaggctg agctggcgaa tcatggcgct gaatgttgag
    40921 gtgagggcag ggaggctctg agataggtgc tggttttgaa taaagagctg aattggattc
    40981 actctgattc ctcaggcact gccggtgccc gcctcatttg ccgcattatt ttgagatttt
    41041 tgcgcgacga tgccgaaatc ctcactttac cgcgattcca tacatcgccc gatgcgttgc
    41101 cccgcccaag ggatatggag gccccagcgt gatcgcgccg gctgcgggca cggccttttt
    41161 ccacaccgtg tagtccacat ggatttcccc gttcgcatcc gctgtcatgc cttccaccac
    41221 ggggttgccc gcccaggggc cgctccgcaa gcggaatccg gtgtcagtga agtcgcgccg
    41281 cacccattcc ggcacctcac cacgggtgtc gagcatcaca tacaccgtgc aggcgcgcga
    41341 aacgctcacc tcgagccgca gatcgcgctc ataccggtcc gccgaaaacg tgccgatcac
    41401 atccgcgccg acgagttctg atggaaattc cgcgcccggc atcgccagcc agcgcgggcg
    41461 accgagcgtc gaatagggcc ggacaccggg cttcatcgcc ccacgctgaa tggtatagaa
    41521 caagtgaaaa ccgtcctcct caatgttgtc tgtcatgccc gtgaccagcg catctggagg
    41581 tggcggttcg agcacaaacg ctgaatctcc acgcgtggcg atgagcgcct gccgacggat
    41641 taatgccact gaattcaccc gcaccgagtc tccagcgctg agggtgacga ggttccgctg
    41701 gtccttcgcg cttttcgggc cacttacctc caccttgccc tgaaacaccg ccacttcagt
    41761 gtcgccatca tccagcaggc tcacgccaaa ccgtgtgccc agatctacca cgtgtgcgct
    41821 cgtggtatcg atggtgaagc catgaccacg ctcacccaca tccgccgtca cactgcccgc
    41881 gagcagcgtg gtgtgcatcg cgtccgcgag gtgcaggcgt acaggagccg tcaagtcgag
    41941 cgtgacgccg gaatctagcc gcaactgcac gctgcctttc tcgagccgca cctcgcgcag
    42001 ccgctgcgtg ctgtctttgg gccagatcga agccgcatcg tccgaaacag ccatcacacg
    42061 cacttccacg ccgcgcggca tccacagcgc caaaacgccc gcgagcaaaa aaacggccgc
    42121 cgcggcccaa cgaacggttt gcgagcgcca gacggtcgtt ttggccttcg cagcacttaa
    42181 aggctgttgc gagaccgggc gcaccatcgt cttcaaggca gcatcgacat gcgcgtagcg
    42241 caaaaattcg cgtcgtagct ccgcatcggc actcagacgg gcatccaggg ccttcacttc
    42301 ctccgccgtc gcacgaccgc tgagatagct ctcgatccaa cggaaatcct tttcgcgttc
    42361 cgtgctcatg ccatgccctc cttcgcgagt tcacgcttcg cacaatcggt gagcatcatg
    42421 cggatgcggt gcagcgattt gtaaaccgcc atcgccgtgc gcccgagccg cgccgccagc
    42481 tcatccatgc gcgagcccgg cgcatacgcc gcctccacga gcgagcgctg gtctgccggc
    42541 agcttttgca ggcaggtgtc cagcgccgtc cgttccttgg cgaattgatc cgccaattcc
    42601 tccgcctcat ccgcgagcgt ggcgaacaac tcatctccaa acacatgccg gtcacggctc
    42661 atcttccgcc gaaactccag cgactccagc cgtgcgacgc cgaaggccca gcgccggaaa
    42721 tcctccggcg cggagagcgt gtgaaagcgc ttccaaagcg ccatcgccac ctcctgcatc
    42781 acatcatccg catccaccag cgtctgcacg agcgagcgca caaagccgcg gatcgacggc
    42841 tcatgctcca cataaaggcg gaggaagttt gcgtgttgct ggaagttggg atcgggcatt
    42901 ttgcagggac actgccgagc acgggagcgt ttgccgcatt ttttgccgcc ccctagtttt
    42961 cgggggggga gcggctaagt ctgtcgccac cgaggcgtat gcttctcact ccaccatgac
    43021 tttctgtttt cgttttcgat cctcattcgc cgccgcgctc accgctagtc ttgcctttgc
    43081 tggtgctggt ttcgctgcgg agccgtatga cgctttcctg gagaaacact gcatcagttg
    43141 tcatggccca gagaaggaaa agggtgactt gcgcatcgac cagctctcgc gggatttcaa
    43201 actcggcgcg gacacacatc attgggcgga ggtgatcgag caggtcaatt caggcgagat
    43261 gccgccaaag aaggagaaga agccgactca ggaagagatc gcggcgtttg tgacgagtct
    43321 tgattcgcgc atcaaagaag gccgcgctgc gcggatggcg gcgcggcctg cggtggcgca
    43381 ttaccggctg agccggaagg agtatcaaaa cacggtctat gacctgctcg gcgtgcgcta
    43441 cgatcccgcg aagccgggcg agctgaatga agacacgcgc tggcatggct ttgagcgcat
    43501 cggctcggaa ctgtcgctgt cgccttcgca tgtggatcgt tattaccgcg ctgcggagct
    43561 ggtgctggag cgcgcgtttc ccgcagcggc ggcgggagag gctcgcaaac ttcgcaagac
    43621 gggggcggag atccgcgatc tggaggcgaa gaagctcctc gacaagacgc tcattgtcat
    43681 caccaccgag ttcggcctcc caccggagtt cgacagcgac ggcgggcgcg gccatcaagg
    43741 cagcgccttc tcctgcgtgc tggccggagg cggcctgcgc cactgcggtg cctggggcga
    43801 gaccgacgag ctctcgaaag agatcgtcgc caaccccttc agcgtgcccg atttcttcgc
    43861 cacgatctgc gccgcggtgg gtgtcgatta cggcaagaac ctatacgacg gcgaccgccc
    43921 cgtgccagtc acggatcagg gcagcccgat tgaggctctt tttgcatagt cagaaaccgg
    43981 gaggctgagg cagccatgaa aacgaacttg tcattcattt tgttgttgtt gacggccgcg
    44041 caggccatcg gcaccgacgg tctgacgctt gatctcgttg tgccgccggg tgcgaaagac
    44101 cgctttcagc acgcggagtt ttccttctgg atcgcggacg cctcgaagcc ggtgaaggtg
    44161 gtcatcattc atcagcacgg ctgcaccaat gcctcgccgg acaagcatcc gccc
//
LOCUS       DAOMYJ010000016        43218 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Prosthecobacter sp. isolate AMR_MDS_5629
            NODE_342_length_43218_cov_23.008850, whole genome shotgun sequence.
ACCESSION   DAOMYJ010000016 DAOMYJ010000000
VERSION     DAOMYJ010000016.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867850
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prosthecobacter sp. (wastewater metagenome)
  ORGANISM  Prosthecobacter sp.
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Verrucomicrobiaceae; Prosthecobacter.
REFERENCE   1  (bases 1 to 43218)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 43218)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.30
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:34:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,742
            CDSs (total)                      :: 4,684
            Genes (coding)                    :: 4,676
            CDSs (with protein)               :: 4,676
            Genes (RNA)                       :: 58
            tRNAs                             :: 55
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..43218
                     /organism="Prosthecobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_342_length_43218_cov_23.008850"
                     /isolate="AMR_MDS_5629"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1965333"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..861
                     /locus_tag="PLP58_03005"
     CDS             <1..861
                     /locus_tag="PLP58_03005"
                     /inference="COORDINATES: protein motif:HMM:NF013916.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ07524.1"
                     /translation="PNPPPTCPPMKNFRLNRLFNPKSGRCFDVAVDHGFFNQPGFLQG
                     IEDMRKVVKTLVDAAPDAIQLTLGQARHLQEMPGRFKPSLVLRTDVANIYGKELPASR
                     FSLMIEETMLQAVRYDAACVCVNLFQIPGAPEVHEQCVENILRLKPQADYYGMPMMVE
                     PLVFQPNEKAGGYMVNGDLTAIMHLVRQAVELGADIIKADPTDDVSLYHQVVETAAGI
                     PVLVRGGGRVSDREILERTQGLLRQGAAGIVYGRNVIQHPNPKGITRALMAMVHDDAS
                     VDEALALVQG"
     gene            complement(875..1669)
                     /locus_tag="PLP58_03010"
     CDS             complement(875..1669)
                     /locus_tag="PLP58_03010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009958808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1295 domain-containing protein"
                     /protein_id="HRJ07525.1"
                     /translation="MPDDPVTLLRTGFAIVLVLFFLTWRLSARKDNYSFVDVTWALAF
                     APLAAWYAVTGAGWLPRRAAIAVLVGLWSLRLGLHLWRRVAEHHPREDARYAVLREKW
                     RAHPRRAFLFFFLAQAVLVWLLMLPVYLIANQPAQGFHALEIAGLALWFGALIGEALA
                     DAQLARFLKSTRDPAAVCDSGLWRYSRHPNYFFQSLLWWGLFLMALPAPWGWAALAAP
                     LAMLHFLLHVTGIPLTEKLSLQKRGEAYRRYQRTTSALVPWFRSAE"
     gene            1839..3455
                     /locus_tag="PLP58_03015"
     CDS             1839..3455
                     /locus_tag="PLP58_03015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009962479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fucose isomerase"
                     /protein_id="HRJ07526.1"
                     /translation="MNTPAKSAKTVQIVASGDLRLAANQTCWPAQKAMEDALAKALKA
                     EGWEVRRSHPYDAKKKHGFIASQREGIEVFRGIDPDAPLIVAEAVWQYSHHVLAGLTT
                     HRGPILTVANWSGQWPGLVGLLNLNASLTKAGVAYSTLWSETFTDAFFKNGLREWLAT
                     GVITHDQSHVRDLDLLKLPADDERLGRDFARRFRARKAILGVFDEGCMGMFNAIVPDH
                     LLHPTGCFKERLSQSTLYAAMREVSDEDAAAVYAWYLKKGLRFALGSDEATELTESQV
                     LQQCKMYIAALRLADEFGCDAIGIQYQQGLKDLTPASDLVEGTLNNADRPPVRGADGR
                     RVLFEGEALPHFNEVDECAGLDGLVTYRLWRELGFAPENTLHDLRWGQKYGGDYVWVL
                     LISGAAPPAHFIGGWKGAGSERQPGMYFRLGGGTLKGVSKPGHIVWSRVFVMEGALHC
                     DIGVAEVVKLPEEETNRRWQETTPQWPIMHAVLKGVTRDQMMARHKSNHIQVVYCPNE
                     KQAHRAARVKAAAMAGLGIKVSLCGDVRLA"
     gene            3504..4265
                     /locus_tag="PLP58_03020"
     CDS             3504..4265
                     /locus_tag="PLP58_03020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012420005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HRJ07527.1"
                     /translation="MKLSTWNVNGIRASLDKGLRDYLLATDADVVCLQETKAQPEQVD
                     LSFLKGWQAAWNSAEKKGYSGTCVLTRAGAPPAVPGMGIKEHDSEGRVITAEHPDFFL
                     VNVYTPNSQAGLARLPYRLRWDDAFRAFLKKLEKKKPVLVCGDLNCAHQEIDLANPKS
                     NRMNPGFSDEERASLTRHLEAGFVDVFRYFDPSPGRYTWWTMRTGGSAREKNIGWRLD
                     YWLASDALRPALSSCVIRSDVFGSDHCPVEVTLDV"
     gene            4282..4662
                     /locus_tag="PLP58_03025"
     CDS             4282..4662
                     /locus_tag="PLP58_03025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ07528.1"
                     /translation="MKHTPLLILAALLACLCACAPEPREAAYSALEWKDDVYFLEGKP
                     FTGIARDAHKDGKPKGEYPFKEGRLHGLVREWWDNGRLSTETNFDSGKRHGLNRYWNL
                     KGQLIKEQVYEHDKSVSEKHYDPK"
     gene            4717..5721
                     /locus_tag="PLP58_03030"
     CDS             4717..5721
                     /locus_tag="PLP58_03030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ07529.1"
                     /translation="MMNTRLLLLPLLASLAACGPKSESPAGDKPAPAPEKQAPAPAEA
                     PPKAPESAAAMPAGDQPFPQAVDINGFGSDEVVPRADMPARGIIRLTSADWQFSNGAH
                     WDTYTGASFKARRWGKYEVMLTYILRRGGIGSQFRMGDLIVKKQLASAQSPRTISLGT
                     LYIPAAGDLPFTLLTPPTEQALVVREITLIPACEGETPRQAADGAITLHAKDATTWSE
                     NMRYEPKPEKNCLGFWTEPDDIAEWEFEVTKPGRYKVVVHHGCGGGNHGSEVEVRLDG
                     QSLKFTTRDTGGFQSWQPVEPGEIEIRKPGKNRLVIDPVNKVKNAVLDVHKVELVPVG
                     "
     gene            complement(5727..6638)
                     /locus_tag="PLP58_03035"
     CDS             complement(5727..6638)
                     /locus_tag="PLP58_03035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009963545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HRJ07530.1"
                     /translation="MHAPLHGLVAATHTPFYNDGTLRLEVIEAQAAHLLRWGVTTLFI
                     NGSTAESSSLTVAERLALAERWFLVTRGTPLRLVVHVGANCVEDSKTLAAQAQGLGAA
                     AIGALAPSYFKPRSVEVLVETMAAISAAAPETPFYYYDIPSLTGVSLPVAAFLEQAPE
                     RIPALAGVKFTNPDLMAYQFCLRAAGGRWDLPWGVDQHFLGALAMGARGAVGSGFNFA
                     APVYHRLMAAFERGDLAAAREEQFRGVRLIQLFDGLGYMAAAKAAMKMLGVDVGPPRL
                     PNARLTPAQESGLRSDLEAMGFFDWVG"
     gene            complement(6853..7323)
                     /locus_tag="PLP58_03040"
     CDS             complement(6853..7323)
                     /locus_tag="PLP58_03040"
                     /inference="COORDINATES: protein motif:HMM:TIGR00022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhcH/YjgK/YiaL family protein"
                     /protein_id="HRJ07531.1"
                     /translation="MVFDTLPHAALYEGLHPHFAKAFAFLRAVDGTQALGRHDLAGDE
                     CFALVQAYDTKPLSEALFEAHRKYIDVQYLHSGCETLLWTPLAAMREETMAYDESKDA
                     ALWRLIPESTPLRMSAGHFAIFYPQDAHAPCIEWQRREAVFKVVVKVAVGDIFP"
     gene            complement(7379..8242)
                     /locus_tag="PLP58_03045"
     CDS             complement(7379..8242)
                     /locus_tag="PLP58_03045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ07532.1"
                     /translation="MSHILDVRTMEKHTLPFPIYALSPNGKEGVSCDFSRVADCRPGY
                     GYAGIKDRFFDDMAPEGSGVTHVNLETGVEKLIVSHKTLANTGTVMENHPTSKHHAYH
                     LLVGPDGKRFIMLHRWTQPKGGHLTRLITANMDGSDLRIVIPNGYASHFIWRDATHIL
                     SQAKGWLGNEDWGDFLFEDKDSGIVEEIGHGILDKSGHLTYLKNNEWILNDTYAQGVR
                     RIQTPHLYHIATKKRIDLGHFPQPPEYKGEWRCDSHPRSSRDEKLVCIDAPDGSNGRQ
                     LFLISIEGLMG"
     gene            complement(8255..8623)
                     /locus_tag="PLP58_03050"
     CDS             complement(8255..8623)
                     /locus_tag="PLP58_03050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ07533.1"
                     /translation="MKSQSNRRHFLSTLALAPLAARGQDAPKFPPTRKITKGPGFHWF
                     AYYDKLQFSPDNRLVLSNKVSFEHRSPTADDVIEVGMVDLQDGDKWIPLGKSNAWNWQ
                     QGCMLQWIPGTESKVIWNDR"
     gene            complement(8645..9352)
                     /locus_tag="PLP58_03055"
     CDS             complement(8645..9352)
                     /locus_tag="PLP58_03055"
                     /inference="COORDINATES: protein motif:HMM:NF018178.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1080 domain-containing protein"
                     /protein_id="HRJ07534.1"
                     /translation="MKPLLISILLATTAFADDVPQWRDLFNGKDLTGWVDVNTSKDTW
                     SVKDGLLVCKGLPIGVMRSEKQYENFILHIEWRHMQAGGNSGVFVWSEGSTPPDRPLP
                     KGMEVQMLELEWPKLHPTKDGQPNHPGYVSGELFGANGLEGVPDNPRGRRSMSWEMRC
                     KGKGEWNTYDVVCVDGTVKLSINGKFVNGISQSTVRKGYLCLESEGAEIHFRNIKIME
                     LPGGRATPEQTAPVVSQ"
     gene            complement(9349..10800)
                     /locus_tag="PLP58_03060"
     CDS             complement(9349..10800)
                     /locus_tag="PLP58_03060"
                     /inference="COORDINATES: protein motif:HMM:NF006560.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HRJ07535.1"
                     /translation="MSPYLRLLAITAFMAASLPLSAHDLVIANGRVMDPATNLDAVRH
                     IAVTGGKISAISETALTGDKVVDAAGLVVSPGFIDLHAHGQTTEDLQIKAQDGVTTAL
                     EMEGGVYPVAAWYASREGKAPIHYGATVGHISARFAAFHPEMRVGQWGGGRSRLALLG
                     PNPAGANKAAGPEEMQRMSTAIREGLDEGALGVGFGINYTPAATPEEIEALFRIAAER
                     KVPAFVHTRAFGIAPIREAIETAKKTGAALHIVHVGSSGISDVPQVLQLIDENRAAGM
                     DLTTEVYPYTAASTMLESAMFNPGWQENLRISYADIVWTATGERLTQESFERYRKQGG
                     WVIMYMMKDANVEKAIAHPGVMIASDGVPFVDGKGHPRGAGTFARVLGLYSREKKLLP
                     LMDALAKMTILPAKRLEAHVPMMKHKGRIAVGADADITVFDAATIIDRATFEAPTTPS
                     AGISHVIVGGTFVVRDGKLVKDALPGRAVRVTP"
     gene            10907..13642
                     /locus_tag="PLP58_03065"
     CDS             10907..13642
                     /locus_tag="PLP58_03065"
                     /inference="COORDINATES: protein motif:HMM:NF024753.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HRJ07536.1"
                     /translation="MKRFLALAVLVFSLNASAEDWPQFMFNAAHSGNAAQIDLDVEKL
                     GLQGAVAMTDGIYTSPVVASGKVYVVDGSGCAACFDAKTLQEVWRFQSKGGKQNVNNV
                     SSPAIAGKWLHFGTTAGFYYVLDRDSGAVVAEIDCGEPIFSTPVVAKDRVYAATLGAQ
                     VLAMTFEGKQLWKWDFVKEVVGFDGNRWSGESWAAFCEKRMLATLKPGTKPTSSDGRV
                     TWKDHFVCSRDICLIKGNIVVIPAGGRTLFLEDLGETPKLKATGEIPEWSGKEFPATF
                     GQSADDEGNVYVQWHRRDNAGRVDIMRLEGDTLKTGDFVPGTDTNIRMDGLLSFSPVA
                     IRGNEVFRVKPEAGRGLIRHDMNTKEFKTFSTRGAISPPVLTKNFVIHGTLDGQILVF
                     PINLNKEEPKNPLTSIIPFRYVETSGEAISAPVAIADSRIYVPSEDGHLYVCGESKGK
                     SQPVVRGLEVSNLRSPLTGKLADAKYDWFTNYGNFAGQNSNSQDLKPPLRMRWARRLE
                     GTVKHLPVCGGGRMYTHTAEGQIIACEQDTGRLLWRKWWPDVYLSFTSPLYIDGKLLV
                     PQAGIKKSFVRCLDAATGNMLWEAPFTGSPSWSRQFPPVVAGNVAIYASGSGDYAPQG
                     SEKPWTFGGELVKPADGREVMSFIYSNDNPYYPANHHPRLWAWDLDTGKVVWEKDFFE
                     FGRGGNDCGICILDGKLYYSVFFGYDADTKRRRGLPVENNGLTCCIDPQTGNILWQTN
                     EYYVTAKCTLSARDGRLFIGGYNRAQQGTDDRFVWCLDANTGKLVWKSDAVTSALNVV
                     SVGERFMFSNALRGKGNIFDRETGKVSYSILTNYACCRFTMSEPYVLGANMDMIDLSQ
                     EGKLVSTGPAIDSRECLGAVVSNGRIFYTSQASGFVVSQTYGPESKDLPPAWEAR"
     gene            13828..13902
                     /locus_tag="PLP58_03070"
     tRNA            13828..13902
                     /locus_tag="PLP58_03070"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:13861..13863,aa:Arg,seq:cct)
     gene            13960..15216
                     /locus_tag="PLP58_03075"
     CDS             13960..15216
                     /locus_tag="PLP58_03075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009964464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HRJ07537.1"
                     /translation="MKRHILFIVLFFVSSAASAANWPMWRGANGDGTCSESDLPFKWS
                     ATENVLWKTRLPEGGNSTPVVWGDKVFVTQAVEKDGRRLLLCFDAKTGKQIWDAGTIY
                     REAELTHKTNPYCAASPATDGELVVVFFASAGVFCYDMSGKEIWKRTDLGKQHHIWGN
                     GTSPVIAGNHVFLNFGPGENTVLYAFDKKTGKTLWQQKEPGGNSGEGGGKNWLGSWSD
                     PLLRKVDARYELIMSWPGRACAYDPMSGRELWTCAGLNPLVYNSPLYADGLVVAFGGY
                     GGMGLAVKAGGEGDVTQSRRVWHLPKVSQRIGSGVLHEGHHYILSDGGIAECRDLKTG
                     EMVWSERLKGPGPTGQNWSSLVLTADKLCYAVNQGGDAFVFRASPKFELLATNSIGEK
                     VIGSIAVSDGRLFIRSYQNLWCIGGR"
     gene            15281..16030
                     /locus_tag="PLP58_03080"
     CDS             15281..16030
                     /locus_tag="PLP58_03080"
                     /inference="COORDINATES: protein motif:HMM:TIGR01490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IB family hydrolase"
                     /protein_id="HRJ07538.1"
                     /translation="MRYAFFDLDHTLLPFDTQTLFCNYVLHRHPWRVVLHLWFLPFAL
                     GRALGLVSTATAKRAFLSYLWGMKRAHLRRLAREFASDCVDSWIYPELRAEILRHKHQ
                     GRKLVLNTASPDFYAHEIAEVLEFDHCVSTRFHIGNEVPLRPVLEGPNNKREAKIAAM
                     REAIPGVRALSDEQRFHCWAYSDSPADIPLLEFAGNRVLVHPGGKLRRHFPQHDVTIL
                     TPRRPYWGRLGDLLAALRQMLGLHGERGPRR"
     gene            16130..16282
                     /locus_tag="PLP58_03085"
     CDS             16130..16282
                     /locus_tag="PLP58_03085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009960041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small basic protein"
                     /protein_id="HRJ07539.1"
                     /translation="MSQHRSLKTSGSVGSKRSVLKRGERIKLMKARGQWQEGRLPTGL
                     PKTKSE"
     gene            16327..17235
                     /locus_tag="PLP58_03090"
     CDS             16327..17235
                     /locus_tag="PLP58_03090"
                     /inference="COORDINATES: protein motif:HMM:TIGR00093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HRJ07540.1"
                     /translation="MRLNRYLSLCGLGSRRGVETLILEGRVCINGHYIRDLATDVSDE
                     DKVTVDGRPARAETPVVLALNKPRGYICSRSDERDRMTIYALLPRQYQHLHHAGRLDK
                     ESEGLLLLTNRGDLSHRLIHPSQGAEKEYEVVTDKPMDLETMNKLVHGMMTEEGHAKA
                     ERAWLASEYRAHVVLKQGLKRQIRLMFYQLGFEVERLRRIRIGWLEIRGLPKGGWREL
                     SEAEVERFFSKEGGTGRASTNTDKPVLKRRAARTEERGAKDERRKPKFEGRKRGERKP
                     GKARPATGRPAFKKPGAKGSRKRPPR"
     gene            17386..18747
                     /gene="asnS"
                     /locus_tag="PLP58_03095"
     CDS             17386..18747
                     /gene="asnS"
                     /locus_tag="PLP58_03095"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012419741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004816 - asparagine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006421 - asparaginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HRJ07541.1"
                     /translation="MHESLSQVLASESTRPALTVRGWVRTKRESKSCAFLEVTDGSCF
                     KSLQVVVDAALPSAALLPRILTGAAVEVEGALVDSPAPGQRWEMQARELRLVGGADAA
                     YPLQKKGHTPEFLRTIAHLRPRTNLFGNVFRVRSRMSYAVHRFFQERGFIHVHTPVIT
                     SNDCEGAGEMFRVTTLKPGGNARAEDDFFGRPAYLTVSGQLQGEAFACALGKIYTFGP
                     TFRAENSNTARHAAEFWMIEPEMAFFDLSADMTLAEENVRYLVKAMLDECGEELEFFG
                     RFVDKTLEARLRQTLEKPFERFSYTEAVDLLLKSGRAFEHPVIWGEGLQTEHERFIAE
                     EHVRGPVTIFDYPKSIKPFYMRQNDDGRTVAAMDLLVPGIGEIIGGSQREERLDVLQE
                     AMRAQGLSEEDYSWYVDLRRYGSVPHAGYGMGFERLLMFVTGVANIRDVIPFARTPGH
                     AEY"
     gene            18894..19712
                     /locus_tag="PLP58_03100"
     CDS             18894..19712
                     /locus_tag="PLP58_03100"
                     /inference="COORDINATES: protein motif:HMM:NF022853.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-disulfide reductase DsbD family protein"
                     /protein_id="HRJ07542.1"
                     /translation="MKIELVPETTAIVPGEAFRAGILIQPEPGWHTYWRQPGIVGVPT
                     RMAWELPEGFSAGELEYPGPEAVLMFHIKAQGFKRDVLLQTRITAPGHLKPGETVTLR
                     GRASWMCCGNICHPGTQILELKMPVASGAEPHERWAPVFERERETFERESSLWEVEAE
                     ENGMTATMKLRPAGAGARLFDGEAGTEGLRFFTEDGWINSDEEQTFTLTNDGALKVGL
                     TRAEVFLGGGGPPERLHGVLRREGGWEMGQSWSCLRIAPKITRRDALKDLQTRR"
     gene            19751..20695
                     /locus_tag="PLP58_03105"
     CDS             19751..20695
                     /locus_tag="PLP58_03105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ07543.1"
                     /translation="MKGILVLILAAAVGYVAYQHVYPIIAEAMKFEKHQPQKQEVAVV
                     APKPEKPEIKVEPPKPAPEPEMKPEPEMKPEPKMVIPEPPPMPEPPKPKEGEFVPPTF
                     DPIEVATQNWSVIPATAFPRQVKMKRDLNLQMKIGASTASTQVKAGGSIYAVGQEGSQ
                     LIVSPTDKSPMRGKVAFEDTDLKEVLTDVYERWKVARTETLRRAHEFKLAAAERAKNA
                     PATATTASASNDKPARDADGTYPLLIASMKSGQVTEIKPESIKEWGEPQMTDGVWTVI
                     VKYETQTMFGKFDTEAQASIKNGKVEKWIYTGSGEVVP"
     gene            20729..21505
                     /locus_tag="PLP58_03110"
     CDS             20729..21505
                     /locus_tag="PLP58_03110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ07544.1"
                     /translation="MNSLLNRLESRLGRFAIPGLVQLIATLQLITLVVLMFLPVEARE
                     PYEALLVFDRALILKGEAWRLFSYVFIPVPNLFFAFIAFMFMRFLGAGLDEGWGAFRV
                     NLYVLGGMAALAAGALVFGFSADAMWVYLAVLFAFATLYPNEEILLFFILPVKIKWVA
                     LFSAVTVALLVFSSPVFAIPAFFALLNYLIVFTPGFVRGRLHAARVAGRRARFEGAAG
                     DGAFFHQCKVCGKTEVDDPALQFRVNDEGDEICSVCRVKP"
     gene            21564..22052
                     /gene="nrdR"
                     /locus_tag="PLP58_03115"
     CDS             21564..22052
                     /gene="nrdR"
                     /locus_tag="PLP58_03115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009962225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140110 - transcription regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NrdR"
                     /protein_id="HRJ07545.1"
                     /translation="MRCPKCGSSQDKVIDSREAREGQAIRRRRECMSCGFRFTTYEIV
                     EREELRVVKRNGARQAFNRDKLMSGLVKACEKRPVTMEQIEQLADDIANELEEEGYRE
                     IPSTAIGAKVMARLEEIDHVAYVRYASVYRQFEDVGEFIDEIRNLDQRRPPDPNQAVL
                     FP"
     gene            22068..22421
                     /locus_tag="PLP58_03120"
     CDS             22068..22421
                     /locus_tag="PLP58_03120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009962226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine triad nucleotide-binding protein"
                     /protein_id="HRJ07546.1"
                     /translation="MPEKTIFQKIIDREIPAPLVYEDDLVAAFNDIAPQAPVHVLIVP
                     KKLIPRVGEAAAEDQAALGALLLAAGRIAEKLGINDTGKGFRLVINHGRDAGESVPHL
                     HVHLLAGRPMEWPPG"
     gene            complement(22491..23492)
                     /locus_tag="PLP58_03125"
     CDS             complement(22491..23492)
                     /locus_tag="PLP58_03125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007417605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HRJ07547.1"
                     /translation="MGRPVTLFTGQWADLSFETMLQKGKNFGYDGLELACWGDHFEVD
                     KADQAYCDAKRAKLKEAGMQCHAISTHLVGQAVCDNIDPRHAQILPPHIYGDGDPEGV
                     RQRAAEELIKTAHAAKRFGVSVVNGFTGSSIWHLVYSFPPNLPGQIDAGYADFARRFK
                     PILDEYQKLGIRYALEVHPTEIAFDIASAERALQALDYHPAFGFNYDPSHFGYQGVDY
                     IKFIYQFKDRIFHVHMKDVAWGIGDGTTGVFGGHVDFHKPSRYWDFKSVGRGNINFER
                     IIRALNDIEYGGPLSVEWEDGAMDREFGATESAAFCKKIDFPPSKIIFDAQFAENSK"
     gene            complement(23610..24476)
                     /locus_tag="PLP58_03130"
     CDS             complement(23610..24476)
                     /locus_tag="PLP58_03130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009959801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4159 domain-containing protein"
                     /protein_id="HRJ07548.1"
                     /translation="MKRPLSALALGLLALVVLAQDSGSPTNFGIKDDYFPQDPREYGR
                     GYFADFPTWPVSRETPDDHFVFARLRYNSESWGRRRGGGGRWTTDYPDADLNFSYRLQ
                     QLTSLQVSPKGAIVDIVPEQLRHYPFIYMIEPGGISLTEDEARILREYLLNGGFMMVD
                     DFWGEQEWDTFYFALKQIFPDREPEELPLEHEIFRMVFPLKVKPQIPSVGHAMAGRSQ
                     GITFERWDAQTPHYKAVYDDKKRMVMIICHNTDLGDGWEEEGTDPWYFREFSEKYAYP
                     LGINIIFYALTH"
     gene            complement(24544..25623)
                     /locus_tag="PLP58_03135"
     CDS             complement(24544..25623)
                     /locus_tag="PLP58_03135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009961118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HRJ07549.1"
                     /translation="MKLFAILACLGASAEALTSAEIPPLRGLSRTDLLEYRDASGAVK
                     RASTTAEWEARRKEALDGFQQMTGPLPGPEARCPLEPQTHEEEDCGGYVRRLISYQSS
                     PGGRVTAYLCVPKAALAGKAAPAVLCLHPTDNVIGHKVVVGLGGRPNRQYAAELAERG
                     FVTLAPSYPLLAQYQPDLKALGFTSGTMKAVWDNIRGLDLLETLPFVKKEAGFATIGH
                     SLGGHNSIFTAVFDERLKAVVTSCGFDSLLDYKDGNIQGWVQERYILKMAEYLGRPQD
                     APFDHYELIACLAPRHVFVSAPLHDSNFKWDSVDRIAAAARPVFALHGAAERLEIHHP
                     DCDHDFPDKERLASYDLIQRVLGVP"
     gene            25711..26121
                     /locus_tag="PLP58_03140"
     CDS             25711..26121
                     /locus_tag="PLP58_03140"
                     /inference="COORDINATES: protein motif:HMM:NF016056.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol-disulfide oxidoreductase DCC family
                     protein"
                     /protein_id="HRJ07550.1"
                     /translation="MNLTPWTNLLLFDGVCHLCDSTVQFVLRHDTSGRIHFCPIQSET
                     GSRLYREHGFDPAAPHTMLLLTPDGALRESDAAIEIARLLGGIWRLALVLKIIPRPLR
                     DAAYRFIASRRYRWFGRHGACLVPLPEWRARFLQ"
     gene            26225..26301
                     /locus_tag="PLP58_03145"
     tRNA            26225..26301
                     /locus_tag="PLP58_03145"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:26259..26261,aa:Pro,seq:cgg)
     gene            complement(26636..27265)
                     /locus_tag="PLP58_03150"
     CDS             complement(26636..27265)
                     /locus_tag="PLP58_03150"
                     /inference="COORDINATES: protein motif:HMM:NF015961.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HRJ07551.1"
                     /translation="MAKIRIQGLGKLVALLIRCVGATLRFEVEDRADAFDRARPGWIW
                     AFWHNRMFVIPYIHDRWFAHIPGAILSSPSGDGQIIADACGEFGFEAARGSSSKPQKG
                     LGALIMLAEKVKEGRDVGITPDGPRGPCYRLQPGILKLAQLTGGVIVPVRVEYCRALR
                     FKTWDRFMLPLPFSRVKVVFEPVVTIPRRLDEADFEKQRLGLQEVLGAD"
     gene            27389..28030
                     /locus_tag="PLP58_03155"
     CDS             27389..28030
                     /locus_tag="PLP58_03155"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HRJ07552.1"
                     /translation="MSAAPVTVLIADDHAVVRRGFARILDSGWDFKVVAEVSNGREAV
                     ASAEELRPDVAVMDVTMPELNGIEATRRLLKVSPKTRVLALSMHKDSVYVREILRAGA
                     KGYLLKDSSEADFLKAMRAVSQGQGYLSPEVADAVLDDYRRHVTHPIDLLSSREREVL
                     QLIAESRTNKEIASALHLSVYTVEAHRGRIMEKLNLHSIGELVRFAMRHGLVD"
     gene            28049..29350
                     /locus_tag="PLP58_03160"
     CDS             28049..29350
                     /locus_tag="PLP58_03160"
                     /inference="COORDINATES: protein motif:HMM:NF019350.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HRJ07553.1"
                     /translation="MRRRLAPFTRRITQRTVLRGLVTGFGLVVLLLGMTGLLAVSESR
                     AIRRGVSGIVREQLLIARLLNEVQAEENTLTEVLHQITRKSQSLDKEALLQDLAVADD
                     AVTRLASDAAATGKAEIWGRLVSSVRSFSSQARAALAGRDAVSEAGLESLFAKHDEVV
                     ALIHGLLRDSTAHLREADAQIEKQSRVLARQSALLLGSSLLMAVLCALGTILFVRHSL
                     RRIEWQAEEINRVSWHMLQSQEETARRFSHELHDELGQSLAAVRANLTSKSHDVSGAR
                     SADCVQLVDEAIANVRELSQLLRPVILDDFGLDAGLRWLAEKFAQRTRLKVDCDSTCP
                     GRFADETETHLFRIAQEALTNIARHAEATRVVIQLALEDKNIRLSVVDDGKGLPDEES
                     KAAPSLGMVGMRARARQCGGELSVSPAPPHGLRIEAVVPLR"
     gene            complement(29368..30303)
                     /locus_tag="PLP58_03165"
     CDS             complement(29368..30303)
                     /locus_tag="PLP58_03165"
                     /inference="COORDINATES: protein motif:HMM:NF013324.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HRJ07554.1"
                     /translation="MIPAAVILIVLVILSASVIKQGHVGVVTRFGKYQRLMLPGLNFK
                     LPLIEGVFKSISTQNQSIELEFEAISLDQAYVNFKSLIVYSAQDAGEETIKKIAFRFI
                     DEKSFMQTLVRSVEGSIRSYVANKKQAEILVLRSEIIGAVKDHLEEALNSWGFHLLDL
                     QINDISFDEAIMRSMSQVVSSNNMKMAAENEAQAQYLIKTRAAEAEARAIRVKAEADK
                     EASELKGAGNARFREQVAEGLANAGKTMQAGDVPPGFMLFTMWLDGMKYISEHGKGNV
                     MFFDGSNEGMERTIKQMQALKTLDQRTVAAANAAH"
     gene            complement(30347..32182)
                     /locus_tag="PLP58_03170"
     CDS             complement(30347..32182)
                     /locus_tag="PLP58_03170"
                     /inference="COORDINATES: protein motif:HMM:NF013109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015116 - sulfate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SulP family inorganic anion transporter"
                     /protein_id="HRJ07555.1"
                     /translation="MSNAAEPALIHPTTASAVDYHPHPRRRKKPLASDLLIAEKAERP
                     AIFTLENLRRDALAGSITGLIAVPLTVGICLMSEYPIETGLLTVLFACLIGFVTFLFR
                     PGNYVGVPGVAAGLAPVLAMSVHKFGMENMPFLIFCTSVMQAIAWKFNLQRFILKAIP
                     SYLIEGLLAGIGLKIALKFLPYTYGILGESHDWFTMERIKMIALSLGALGLFLHLFGK
                     YKAKYPAVPYVAVVLVGVISGIYMHIPMLHIEHSPIKVAWPFPDFDVIPPKMVAEIIG
                     YALMLALIDVIEQVMSNAAIEKLDPLGRPANTNNSLLAIWVANIGSSFFGGMTNLDGL
                     AKSATNALAGAVTKISNLFTAGVILVFILDPHLLEHLPYFSLAVLMIFTGWKMIVGLT
                     HVAAHGSYALLLALFCGILTYTVGIFEGLIVVLGIHLFIHFVIFRHEHMPWIEMLKKI
                     AHKFGDEIDPRSTDTMLVHRDHEVGGLRYSTISHNAAEKKNLTEFINDWAFGINNHSM
                     AAVVGCYDMNGLLWGTFAKELREGHHKIKGYFEHLFELEDVHVRFESGEVRQYKDIYI
                     QSGKYVFTFKRKKELISVPARYSFVCKKEKTGWFILEHHSSEFPA"
     gene            complement(32266..33027)
                     /locus_tag="PLP58_03175"
     CDS             complement(32266..33027)
                     /locus_tag="PLP58_03175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007417877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1080 domain-containing protein"
                     /protein_id="HRJ07556.1"
                     /translation="MKTPAFFSLCVLAAFALPSCKPSAPSGGSGPASSSTVEVFNGKD
                     LAGWEGNLDLWSVKDGAITGITPPDPANPEKGIIKHNTFLVWKGGTVSDFELEFQYRI
                     EKGNSGVQYRSKELAPGEFGPVISGYQADFEAGDKYSGILYEEKGRGILALRGEKTVI
                     KPGQDPKKPAVEKAGTVGDSEAIQQSIKKEDWNDYKIIAKGNHIQHFINGMQTVDVTD
                     EDAANAPKEGLLALQIHQGPPMVVQFKNFRLTPLK"
     gene            complement(33083..33928)
                     /locus_tag="PLP58_03180"
     CDS             complement(33083..33928)
                     /locus_tag="PLP58_03180"
                     /inference="COORDINATES: protein motif:HMM:NF024242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HRJ07557.1"
                     /translation="MNTRRKYLNYTTPWGVSLARVSGAGWSDFLLHECGYQAALEDWN
                     HDGVDSPFWRFYHNPKPGCFVHFRGRQIALEPSTCMIIPADTVFDCRGPVPACHLWMH
                     FTVNRPGGPVDPAPLLLPMDALLTTLAQSVIALHHEHASDTRDHRLLHESSALLYAAF
                     ARLSLPAPPPMPERLLGVLSLVQRAPHADLSNRLLAARAGMSLERFIRAFREHTGSTP
                     AAYVSAARVRHAQQLLALTDKTVDQIALESGFPNRHYLTRVFSRHTGCGPAEFRARQH
                     RRKGR"
     gene            34253..37267
                     /locus_tag="PLP58_03185"
     CDS             34253..37267
                     /locus_tag="PLP58_03185"
                     /inference="COORDINATES: protein motif:HMM:NF019223.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1553 domain-containing protein"
                     /protein_id="HRJ07558.1"
                     /translation="MPAFTQGADKLRFNRDIRPILSDKCFHCHGFDPKTRKAGLRLDT
                     FEGATEDGGIVAGRAERSGIIERVLSHDPDEAMPPPESKIGRLSDTEIATLKRWINEG
                     AEYEAHWAFVPLDKAQLDEWTRQAKAAGVASPMDWIVSEGLKAHGLAMQPRADAATLA
                     RRVSFDLTGLPPSVEELADVELESLITKKLASPAYGERMAADWLDIARYADSFGFQVD
                     REREVWMWRDWVVRAFNDNLPFDQFITWQLAGDLLPQVTDEQVLATAFNRLHQQESEG
                     GSVEEEYRIEYVADRVQTVATAFLGLTFECARCHDHKYDPVSQREYYGMASFFQNIDE
                     AGLYSFFTSAPPTPAMMLMDAPAKEKMTALKARVAALEKQLASTGVKPLAEIPLRDGV
                     PVSLEGQLAHFSFDALEGNKLADSLNPESPPAPAPKKADPKTKDKAPPGPSAILKGEN
                     KLVQGRLGQAVQFTGDDPVDTPVGNFRRHEPFSVSLWMKTPDVKERAVVFHRSRAWTD
                     AASRGYELLIEEGRLKWSLIHFWPGNAISIRACDPLPVNEWAHVIVTNDGSSRASGLR
                     LFINGKAVPVDVIRDGLTQDITGGGGDTISLGERFRDRGFKGGLIDDFRVFQRDLSMP
                     VRPELEPLLAELKAARGELTDFSGQQKEIMVMRELPEPKKAYVLTRGEYDKPTEEVTA
                     MTPASLPPFPQDAPKNRLGLARWLTDPGHPLTARVTVNRVWQSLFGRGLVKTSEDFGS
                     QGEKPLYPELLDYLSLRLIHSGWDMKALLREIVCSAVYQQRSIAEKQVMEDDPENQWL
                     ARGPAFRLPAEMIRDNALAAAGLLNPKMGGPPVNPYEMAEAFKPFQVAKEGESLYRRS
                     LYTTWRRTSPPPAMLAFDAPRRAVCSAKRERTESPLQALILLNGTQFVEAARVLGENL
                     HHEHGGAVEKMIRHGFLRCLSRQPDTREMEICTRLYQEQLAYFRAHLQEAVELMKTGG
                     APRVAGVPDVEGAAAAALAQALLNHDACVVKR"
     gene            37340..37780
                     /locus_tag="PLP58_03190"
     CDS             37340..37780
                     /locus_tag="PLP58_03190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009960980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GxxExxY protein"
                     /protein_id="HRJ07559.1"
                     /translation="MAAFNHKPHDMRIHKNHRIEMREDGPFQQEGYDLMAAAFEVHNE
                     NGGGLAEEVYQESFEIELEMRGMLFVAKQELALFYKGRELKRRYIPDLYVCEGIVVEL
                     KSVAQLLPEHSAQLFNYMRISGKSVGYLINFAPIKRIEWRRYVL"
     gene            37971..39347
                     /locus_tag="PLP58_03195"
     CDS             37971..39347
                     /locus_tag="PLP58_03195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009960912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1501 domain-containing protein"
                     /protein_id="HRJ07560.1"
                     /translation="MGLGGLALAELLMREQAGASPVDAGVLGGTHWQPKAKRIIYLFM
                     SGGPSHLDLYDYKPLLNQRHGEQLPDSVRGGQRLTGMSGNQSSIPMVGSPFKFTRHGQ
                     AGAWFSELLPHTASIADDLCVVRSMFTESINHGPGVTFFQTGSQIAGRPSMGAWLSYG
                     LGAENANLPSFTVLITKDKGGQPLGAHLWGSGFLPTKHQGVLFRAAKDPVLYLGNPAG
                     VSADSRRLMLDRLRELHEHQYAGTPDVEIQNRMDHYEMAWRMQSSIPEATDIGDEPQH
                     VLDMYGPDVTKPGTFAANCLQARRLAERGVRFIQLYHQDWDHHGGLPGALPRLCKETD
                     QASAALVKDLKQRGLLEDTLVVWGGEFGRTNYCQGKIQANFGRDHHPRCFSVWMAGGG
                     VKPGAVYGETDEFGYNVARDGVHIHDFHATLMHLLGIDHERLTYKFQGRRYRLTDVHG
                     HVVKGLLA"
     gene            39388..40074
                     /locus_tag="PLP58_03200"
     CDS             39388..40074
                     /locus_tag="PLP58_03200"
                     /inference="COORDINATES: protein motif:HMM:NF014625.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L family deacetylase"
                     /protein_id="HRJ07561.1"
                     /translation="MPKTLLAIGAHYDDCPFGIPGILLKAVREHHRVVILSLIGDYTN
                     WPPVQGREQALLDKTAAMAAERGMEMRFLKHASMGFEPTLATKREVAEIVAEVRPDTA
                     FMLWRQDRHPDHEAASAICHDSLFQPARLTGNANARSPSQVFWYDNGPGHTIDFTPDT
                     YVDVSAEWSGVVEWLGGLMAFVRNGPFDPQKDAAVEAKTVLARYRGLACGVKHAEALR
                     SVRPLSREVW"
     gene            complement(40106..41545)
                     /locus_tag="PLP58_03205"
     CDS             complement(40106..41545)
                     /locus_tag="PLP58_03205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009958418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1501 domain-containing protein"
                     /protein_id="HRJ07562.1"
                     /translation="MSPHFESLHLRTRRQFLTGAGQFSLGAIALHAFDSGARAAPGPL
                     SARHPHHTAKAKRVVYLHMSGAPPHLDLFDYKPELVRRDGQDCPDSFLKGRTFAFTSG
                     VPKLMGTPRKFARHGRAGMWMSDACPNFHTLADEITLIRSMSTDQFNHAPAELLLFTG
                     HTRQGRPSMGSWATYGLGTENQDLPGFVVLISSGTQPSGGQSCWGSGFIPSVYQGVQC
                     RSQGDPVLFVNNPPGMDRALRRKTLDVLRELNERQAAELVHPETRTRIEQYELAYRMQ
                     ASVPEVMDISREPARVIEEYGAKPGAASFANNCLLARRLVEQGVRFVHLFDWGWDFHG
                     TSEATGITDGLTKKMAETDKPVAALIKDLKQRGLLDDTLVIWGGEFGRTPFREGRTAA
                     SKVLGRDHYPDCFTLFMAGGGVKAGFDYGATDEMGFAVAEHKVHVHDFQATVMHLLGF
                     DHERLTHRFQGRDYRLTDIHGHVVKDILA"
     gene            complement(41694..42608)
                     /locus_tag="PLP58_03210"
     CDS             complement(41694..42608)
                     /locus_tag="PLP58_03210"
                     /inference="COORDINATES: protein motif:HMM:NF012970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ07563.1"
                     /translation="MNDSAARTLCCLSIMSLAAAQASDLPSENLESPPPVTAKSWVIA
                     DLESGAVLQSLLPDEPRKAASTTKTMCAFVVLELALKDPAVLDEWVAVTKLAASTQGS
                     AAELKEGEQVRVRDGLFALMLPSGNDMGNAFAEHFHPRLQPPGDETPERVKTPDYATR
                     ANFIAEMNRTARRLGMNSTTYRISYGDGGEANDYTTTARDLVTLTLAARKHALFREVV
                     ATAARTGKVRLPDGGERDAEWRNTNELLQLANYDGVKTGTTPSAGRCLVATGSHNGRG
                     LIVVTLGSTSDEARFTDSRNLFRWAWGR"
     gene            complement(42633..>43218)
                     /locus_tag="PLP58_03215"
     CDS             complement(42633..>43218)
                     /locus_tag="PLP58_03215"
                     /inference="COORDINATES: protein motif:HMM:NF015535.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="P1 family peptidase"
                     /protein_id="HRJ07564.1"
                     /translation="AFGWKGGIGTASRVLPARDGGHTVGVLAQSNFGGVFTLDGAPVD
                     KVLGRHFMREAYDGADGSCMIVVATDAPLDARNLRRLAARAMLGLGRTGGIAANGSGD
                     YVIAFSTARELRIPHKHEGPLNPPAALMRNEAMTPLFLAVIEAAEEAILNSLFMAADM
                     TGHRGRAVKAMPVDEVLKIWREHRPAGGSSVKKP"
BASE COUNT         8069 a        13495 c        13755 g         7899 t
ORIGIN      
        1 cccaaccccc cgccaacctg cccgcccatg aaaaacttcc gcctcaaccg cctcttcaac
       61 cccaagtcgg gccgctgctt cgacgtcgcc gtggaccatg gtttcttcaa ccagcccggt
      121 ttcctccagg gcatcgagga catgcgcaag gtggtcaaga ctctcgtgga cgccgcgccg
      181 gacgccatcc agctcaccct cggccaggct cgtcatttgc aggaaatgcc cggccgtttc
      241 aagcccagcc tcgtcctgcg caccgatgtg gcgaacatct acggcaagga gctgcccgcc
      301 tcccgcttca gcctcatgat cgaggagacc atgctccagg ccgtgcgcta tgacgcggcg
      361 tgcgtttgtg tgaacctttt ccaaatcccc ggcgcgccgg aggtgcatga gcagtgcgtg
      421 gaaaacatcc tgcgcctcaa gccccaggcc gactactacg gcatgcctat gatggtggag
      481 ccgctcgtct tccagccgaa cgagaaagcc ggcggctaca tggtcaatgg cgacctcacc
      541 gccatcatgc acctcgtgcg ccaggccgtg gagctgggcg cggacatcat caaggcggac
      601 cccaccgatg atgtgagcct ttaccatcaa gtcgtcgaaa ccgccgccgg cattcccgtg
      661 ctcgtgcgcg gcggcggccg cgtcagcgac cgcgagatcc tcgagcgcac ccagggcctg
      721 ctccggcagg gcgcggcggg catcgtctat ggccgcaatg tcatccagca tccgaacccg
      781 aaaggcatca cccgcgcctt gatggccatg gtccacgacg acgccagtgt ggacgaggcc
      841 ctggctctcg tgcaggggtg agcggcgcgc cgcctcactc cgcggaccgg aaccacggca
      901 cgagggcgct ggtggtgcgc tggtagcggc ggtaggcctc gccgcgtttt tgcagggaga
      961 gtttttcagt cagcgggatg ccggtgacat gaagcaggaa atgcagcatc gccagcggcg
     1021 cggccagcgc cgcccagccc cagggcgccg gcagcgccat caaaaacagg ccccaccaga
     1081 ggagcgactg gaagaaatag ttcggatgcc tggagtagcg ccacagtccc gaatcgcaca
     1141 cggcggccgg gtcgcgcgtg gatttcaaaa agcgcgcgag ctgcgcgtcg gccagggctt
     1201 ctccgatcag cgcgccgaac cagagcgcga gaccggcgat ctccagcgcg tgaaaaccct
     1261 gagcgggctg gttcgcgatc agataaaccg gcagcatgag cagccagacg agcacggcct
     1321 gggccaggaa gaaaaacaga aaggcgcgcc ggggatgcgc gcgccatttc tcccgcagca
     1381 cggcgtagcg cgcgtcctcg cggggatgat gctcggccac tcgccgccac aagtgcagtc
     1441 cgaggcgcag gctccacagc ccgacgagaa cggcgatggc cgcgcggcgc ggcagccagc
     1501 ccgcgccagt cacggcatac cacgcggcca gcggtgcgaa tgcgagcgcc caggtgacgt
     1561 caacgaagga gtagttgtcc ttgcgcgcgc tcagccgcca ggtcaggaaa aacagcacga
     1621 gcacgatggc gaagccggtg cgcaggaggg tgacgggatc atcgggcatg acctgggata
     1681 cgcgcgtggc gcgggccggg cttcttttgt ccaaaaatcg ctggaaggcc ggtcaccgcc
     1741 ctcaagtcaa gaatgcacat gcggggtgca agacacggca tgagcgctgt cttgttgccc
     1801 ggcgcgcggc cttgcaaaat gaccgccact ttcccgccat gaacacccca gccaaatccg
     1861 ccaagaccgt ccaaatcgtc gccagtggcg atctccgcct tgccgccaac cagacttgct
     1921 ggccggcgca gaaagccatg gaggacgcgc tcgccaaagc cctcaaggcc gagggttggg
     1981 aagtgcggcg ctcgcatccg tacgatgcca aaaagaagca cggcttcatc gccagccagc
     2041 gggagggcat cgaggtcttt cgcggcattg acccggacgc gccgctcatc gtcgccgagg
     2101 ctgtgtggca gtactcgcat catgtgctgg ccgggctgac cacgcaccgc ggccccatat
     2161 tgaccgtggc gaactggagc ggccagtggc ccggtctcgt cggcctgctc aacctcaacg
     2221 cctccctcac caaggccggc gtggcctact ccaccctgtg gagcgagacc ttcaccgacg
     2281 cctttttcaa aaacggcctg cgcgagtggc tcgccaccgg ggtcatcacg cacgaccagt
     2341 cccatgtgcg cgatcttgat ctgctcaagc tgccagccga cgacgagcgt ctcgggcgcg
     2401 acttcgcccg ccggttccgc gcgcgcaaag ccatcctcgg cgtcttcgac gagggctgca
     2461 tgggcatgtt caacgccatc gtgcccgacc acctgctgca ccccaccggc tgcttcaagg
     2521 agcgcctcag ccagtccacc ctctacgccg ccatgcgcga ggtgagcgat gaggacgccg
     2581 ccgccgtcta tgcgtggtat ctgaaaaaag gcctgcgctt tgcccttggc agtgacgagg
     2641 caaccgagct caccgagtcg caggtgctgc agcaatgcaa aatgtacatc gccgccctgc
     2701 gtctggccga cgagttcggc tgcgacgcca tcggcatcca ataccagcag gggctgaagg
     2761 atctcacacc cgccagcgac ctcgtggagg gcacgctcaa caacgcggac cgcccgcccg
     2821 tgcgcggcgc cgacggccgg cgcgtgctct tcgagggcga ggcgctgccg catttcaacg
     2881 aggtggacga atgcgccggc ctcgacggcc tcgtcaccta ccgcctgtgg cgcgagcttg
     2941 gcttcgcccc tgagaacacc ctgcacgacc tgcgctgggg tcaaaagtac ggcggcgact
     3001 atgtctgggt gctgctcatc agcggcgccg cgccgcccgc gcacttcatc ggcggctgga
     3061 agggggctgg cagcgagcgc cagcccggca tgtatttccg gctcggcggc ggcacgctga
     3121 agggtgtgag caagccgggg cacatcgtct ggagccgcgt cttcgtcatg gagggcgcgc
     3181 tgcattgcga catcggcgtg gccgaggtgg tgaagctgcc ggaggaggaa acaaaccgcc
     3241 gctggcagga gaccacgccg cagtggccca tcatgcatgc cgtgctcaag ggcgtgactc
     3301 gggaccagat gatggcccgg cacaagtcga accacatcca ggtcgtctac tgcccgaacg
     3361 aaaagcaggc ccaccgcgcc gcccgcgtga aggccgccgc catggcgggg ctggggatca
     3421 aggtcagcct gtgcggagac gtgcgcctcg cctgagccgc atccccgctc gacatccccg
     3481 ccattcccgc ccaaaagtgg cggatgaagc tctccacctg gaatgtgaac ggcatccgcg
     3541 cctccctcga caaagggctg cgagactacc ttctggccac cgacgccgat gtcgtctgcc
     3601 tccaggagac caaggcgcag cctgagcagg tggacctgtc ctttctcaaa ggctggcagg
     3661 ccgcgtggaa cagcgccgaa aaaaagggct actccggcac ctgcgtcctc acccgcgccg
     3721 gcgcgccgcc cgccgttccc ggcatgggca tcaaggagca tgacagcgaa ggccgcgtca
     3781 ttaccgccga gcatccggat ttcttcctgg tcaatgtcta tactccgaac agccaggccg
     3841 ggctggcccg cctgccttac cgcctgcgct gggacgacgc cttccgcgcc ttcctgaaaa
     3901 agctggagaa aaaaaagccc gttcttgtct gcggcgacct caactgcgcg caccaggaga
     3961 tagacctcgc caaccccaag tccaaccgca tgaacccagg cttcagcgac gaggagcgcg
     4021 ccagcctcac ccgccacctg gaggccggct ttgtggatgt cttccgctac ttcgacccca
     4081 gccccggacg ctacacctgg tggaccatgc gcaccggcgg cagcgcgcgg gaaaagaaca
     4141 tcggctggcg gctcgactac tggctggcct ccgacgcgct ccgccccgcc ctctcctcct
     4201 gcgtcatccg ctccgatgtc tttggcagcg accactgccc cgtcgaggtc acgctcgatg
     4261 tctgaaaaag accgcccgcc catgaaacac acgcccctcc tcatcctcgc cgccctcctc
     4321 gcctgcctgt gcgcctgcgc gcccgaaccg cgcgaggccg cctacagcgc gctcgaatgg
     4381 aaggacgacg tttatttcct cgaaggcaaa cccttcaccg gcatcgcccg cgacgcgcac
     4441 aaggacggca aacccaaggg cgaatacccc ttcaaggagg gccgcctgca cggcctcgtg
     4501 cgcgagtggt gggacaacgg ccggctctcc accgagacga atttcgactc cggcaagcgc
     4561 cacggcctca accgctactg gaatctcaaa ggccagctca tcaaagagca ggtctatgag
     4621 catgacaaat ccgtcagcga gaagcactac gaccccaagt aatgcgctaa cgacaccccg
     4681 cccggcacgt ttcctccacc ccgccgcacc cccatcatga tgaacacccg cctcctgctc
     4741 ctgcccctac tcgccagcct cgccgcctgc ggccccaaat ccgaaagccc cgctggcgac
     4801 aaacccgcgc ccgccccgga aaagcaagcc cccgcacccg ccgaagcacc gcccaaggcc
     4861 ccggaaagcg ccgccgccat gcccgccggc gaccagccct ttccccaagc cgtggacatc
     4921 aacggcttcg gcagtgacga ggtcgtgccc cgcgccgaca tgcccgcgcg cggcatcatc
     4981 cgcctcacct ccgccgactg gcagttcagc aacggcgccc actgggacac ctacacgggc
     5041 gcgagtttca aggccagacg ctggggcaaa tacgaggtca tgctcaccta catcctgcgc
     5101 cgcggcggca tcggctccca gttccgcatg ggtgatctca tcgtcaaaaa acagctcgcc
     5161 tccgcgcaga gcccccgcac catcagcctc ggcacgcttt acatccccgc cgccggagac
     5221 ctgcccttca ccctgctcac cccgcccacc gagcaggcgc tcgtcgtccg ggagatcacc
     5281 ctcatccccg cctgcgaggg cgagacgccc aggcaggccg ccgacggcgc catcaccctc
     5341 cacgcgaagg acgccaccac ctggtcggag aacatgcgct acgagcccaa gcccgaaaaa
     5401 aactgcctcg gcttctggac cgagccggac gacatcgccg agtgggagtt cgaggtcaca
     5461 aaacccggcc gctacaaagt cgtcgtccac cacggctgcg gcggcggcaa ccacggcagc
     5521 gaggtcgagg tcaggctcga cggccagagc ctcaaattca cgacccggga caccggcggc
     5581 ttccagagct ggcagcctgt cgagccgggc gagatcgaga tcaggaagcc cggcaaaaac
     5641 cgcctcgtca tagacccggt caacaaagtg aaaaacgccg tgctcgacgt tcacaaagtc
     5701 gaactcgtgc ccgtgggctg aattttttac cctacccagt cgaaaaaccc catcgcctcc
     5761 agatcggagc gcagccccga ctcctgcgcg ggcgtcaggc gcgcgttcgg caggcgcggc
     5821 ggccccacgt ccacgcccag cattttcatc gccgccttcg ccgccgccat gtagcccagg
     5881 ccgtcaaaga gctggatcag ccgcactccg cgaaactgct cctcccgcgc cgccgccaga
     5941 tccccgcgtt cgaaggccgc catcagccgg tggtaaaccg gcgcggcaaa attaaacccg
     6001 ctgcccaccg ccccgcgcgc ccccatcgcc agcgctccca ggaaatgctg gtccacgccc
     6061 cagggcaggt cccagcgccc gcccgccgcg cgcaggcaga attgatacgc catcagatct
     6121 ggattcgtga acttcacccc cgccagtgcc gggatgcgct ccggcgcctg ctccagaaaa
     6181 gccgccactg gcaggctcac tcccgtcagc gaggggatgt cataataata aaagggcgtc
     6241 tccggtgccg ccgccgaaat cgccgccatc gtctccacca gcacctccac cgagcgcggc
     6301 ttgaaatacg agggtgcaag cgccccgatg gcagccgccc ccagtccctg tgcctgcgcg
     6361 gcaagcgtct tcgagtcctc cacacaattc gcgcccacat gcaccaccag tcgcagcggc
     6421 gtgccgcgcg tcaccaggaa ccagcgctcc gccagcgcca gcctctccgc caccgtcagc
     6481 gagctactct ccgccgtgct gccattgatg aacagagtcg tcacccccca cctcagcaga
     6541 tgcgccgcct gcgcctcgat gacctccagc ctcagagtgc cgtcattata aaaaggagtg
     6601 tgagtggcgg ccaccaggcc gtgcaaagga gcgtgcatca ctccagcaaa cgctcagccg
     6661 ccccgcgcgc aatcagccca tcgtggtcaa aagcgcggac ctgcgcgccg tgccgttgac
     6721 tgattttata atgactgccg actgaaaatc agccgccgct gactgatttt taatgaccaa
     6781 acaacggaaa atcagtgatt tacctccatg agcgacggag tcggtctctt ggacgtgggt
     6841 atttcacgtc atttaaggaa aaatatcgcc caccgccacc ttcaccacga ctttgaaaac
     6901 ggcctcccgg cgctgccatt cgatgcaggg ggcgtgggcg tcctgggggt aaaagatggc
     6961 gaagtgcccg gcggacatgc gcaggggcgt ggactcgggg atgagacgcc agagggcggc
     7021 gtccttggac tcatcgtagg ccatggtttc ctcccgcatg gcggccaggg gcgtccagag
     7081 gagggtctcg cagccgctgt ggaggtattg cacgtcaatg tatttccggt gcgcctcgaa
     7141 gagggcttcg gacagcggct tggtgtcata ggcctgcacc agggcgaagc attcgtcacc
     7201 cgccaggtcg tgccgtccca gggcctgggt gccatccacc gcgcgcagga aggcgaaggc
     7261 tttggcaaag tgcgggtgca gcccttcgta aagggcggcg tgggggagcg tgtcgaagac
     7321 catgcctcca gggtagggtg gccgcgcgcc ggtgcaaccg gcgctttgcc ctggggcgtc
     7381 atcccatcag cccctcgatg ctgatgagga aaagctggcg gccatttgag ccatccgggg
     7441 catcaatgca gacgagcttt tcatcgcggc tgctgcgcgg gtggctgtcg cagcgccatt
     7501 cgcccttgta ttccggcggc tgcgggaagt ggcccaggtc aatgcgcttc ttggtggcga
     7561 tgtgatacag atgcggcgtc tggatgcggc gcacgccctg cgcgtaggtg tcgttgagga
     7621 tccactcgtt gttcttgagg taggtgagat ggccggactt gtcgagaatg ccgtggccga
     7681 tctcctccac gatgccgctg tccttgtcct cgaagaggaa atcgccccag tcctcgttgc
     7741 cgagccagcc tttggcctgg gagaggatgt gggtggcatc ccgccagatg aaatgggagg
     7801 cgtagccgtt cgggatgacg atgcgcaggt cgctgccgtc catgttcgcc gtgatcaggc
     7861 gcgtgagatg gccgcccttc ggctgcgtcc agcggtgcag catgatgaag cgctttccat
     7921 ccggccccac gagcaggtga taggcgtgat gcttcgaggt cggatgattt tccatcaccg
     7981 tgcccgtgtt cgcgagcgtc ttgtggctga cgatgagctt ctcgacgccg gtctccaggt
     8041 tcacatgcgt cacgccgctg ccttccgggg ccatgtcgtc aaagaagcgg tctttgatgc
     8101 cggcgtagcc atagccgggg cggcagtccg ccacgcgcga gaagtcgcag ctcacacctt
     8161 ccttgccgtt cgggctcaag gcgtagatcg ggaaaggcag cgtgtgcttc tccatcgttc
     8221 tcacatcgag gatgtggctc acgaacttgt ccttctaccg gtcgttccag atcaccttcg
     8281 actccgtgcc gggaatccat tgcagcatgc agccctgctg ccagttccac gcgttggatt
     8341 tgccgagggg aatccatttg tcgccgtcct ggaggtccac catgcccacc tcgatcacgt
     8401 catccgccgt cggcgagcgg tgctcgaagc tgaccttgtt tgagagcacg aggcggttgt
     8461 ccggcgaaaa ctgcagcttg tcgtagtagg cgaaccagtg gaagccgggg cctttggtga
     8521 tcttgcgtgt cggcggaaac ttcggcgcgt cctggccgcg tgcggcgagc ggtgcgagag
     8581 cgagcgtgga gaggaaatgg cggcggttgg attgtgactt catcgaggga gatggaaaaa
     8641 atgattactg actgaccacc ggagccgtct gctccggtgt ggcgcggccg ccgggcagtt
     8701 ccatgatctt gatgttgcgg aagtggatct cggcgccctc ggattcgagg cagaggtagc
     8761 ctttgcggac ggtggactgg ctgatgccgt tgacgaactt gccgttgatc gaaagcttca
     8821 cggtgccatc gacacacacg acgtcgtagg tgttccattc gcctttccct ttgcagcgca
     8881 tctcccacga catgctgcgg cggccgcgtg gattgtcggg aacgccttcg aggccgttcg
     8941 cgccgaaaag ttcgccggag acgtagccgg gatgattcgg ctggccgtcc ttggtgggat
     9001 gcagcttggg ccattcgagt tcgagcatct gcacctccat gcccttcggc agcggacggt
     9061 cgggcggcgt gctgccctcg ctccagacaa agacgccgct gttgccaccg gcctgcatgt
     9121 gccgccactc gatgtgcagg atgaagttct catactgctt ctcgctgcgc atcacgccga
     9181 tgggcaggcc tttgcagacg agcaggccgt ctttgacgct ccaagtgtct ttgctggtgt
     9241 tcacatccac ccagccggtg aggtccttgc cgttgaagag gtcgcgccac tgcggcacgt
     9301 cgtcggcgaa ggcggtggtg gcgaggagga tggaaatgag gagcggcttc atggtgtgac
     9361 tctcacagcg cggcccggaa gggcatcttt cacgagtttg ccatcccgca cgacgaaagt
     9421 gccacccacg atgacgtgtg aaatgcccgc cgagggcgtg gtgggtgcct caaaggtggc
     9481 gcggtcgatg atggtggcgg cgtcaaagac cgtgatgtcc gcgtcggcac cgacggcgat
     9541 gcggcctttg tgtttcatca tcgggacgtg ggcttcgagg cgcttcgcgg gcaggatggt
     9601 catcttcgcg agcgcatcca tgagcggcag gagcttcttc tcgcgactgt aaaggcccag
     9661 cacgcgtgcg aaggtgcccg cgccgcgtgg gtggcctttg ccatcgacaa acggcacgcc
     9721 gtcgctcgcg atcatgacgc cgggatgcgc gatggccttc tccacattcg cgtctttcat
     9781 catgtacatg atcacccagc cgccctgctt gcggtagcgt tcaaaactct cctgcgtgag
     9841 ccgttcgccc gtcgccgtcc acacgatgtc cgcgtagctg atgcgcagat tttcctgcca
     9901 gccggggttg aacatcgcgg actccagcat cgtggacgcg gcggtgtagg gatagacctc
     9961 cgtggtcaga tccatgcctg cggcgcgatt ttcgtcaatc aattgaagca cctgcggcac
    10021 atcggagatg ccgctgctgc cgacatgcac gatgtgcagc gccgcgcctg tcttttttgc
    10081 cgtttcgatg gcctcgcgga tcggcgcgat gccaaacgcg cgagtgtgga cgaacgcggg
    10141 caccttccgc tccgccgcga tacggaacag cgcctcgatc tcctccggcg tggccgcggg
    10201 cgtgtagttg atgccgaaac cgacacccag cgcgccttcg tcgagccctt cgcggatcgc
    10261 cgtgctcatg cgctgcattt cctccggccc cgccgccttg tttgccccgg ctgggttcgg
    10321 ccccagcagc gccagcctgc tcctgccgcc gccccattgg cccacgcgca tctccgggtg
    10381 aaacgccgcg aaacgcgccg agatgtgccc caccgtcgct ccataatgaa tcggcgcctt
    10441 gccctctctc gacgcatacc acgccgccac aggatacacg ccaccctcca tctccagcgc
    10501 cgtcgtcacg ccgtcctgcg ccttgatctg caaatcctcc gtcgtctgcc cgtgcgcatg
    10561 caggtcgatg aatcccggcg aaacgacgag tccggccgca tcgaccactt tgtcacccgt
    10621 gagcgccgtt tcggagatcg cgctgatttt tccacccgtg acagcaatgt gccgcaccgc
    10681 gtcgagattc gtcgccgggt ccatgacgcg gccattggcg atgacgagat cgtgggcgga
    10741 aagcggaagc gaggcggcca taaaagctgt gatggccagg agacgcaggt atgggctcat
    10801 ggtcggatga ataacaggta tcgtcaccgc agacaagggg gccgctgaac cgtggcaggt
    10861 acagatgaaa tgcgagttca gccgctgcgg ctcgtattca ctggccatga agcgcttcct
    10921 tgccctcgca gtccttgttt tcagcctgaa cgcctccgcg gaggactggc cgcagttcat
    10981 gttcaatgcc gcgcacagcg gcaatgcggc tcaaatcgac ctcgatgtcg agaaactcgg
    11041 cctgcaaggc gcggtggcga tgacggatgg catttacacc tcgccggtgg tggcaagcgg
    11101 caaggtgtat gtggtcgatg gatcgggctg tgcggcatgt tttgatgcga aaacactcca
    11161 agaggtctgg cgcttccaaa gcaagggcgg caaacaaaac gtcaacaacg tcagttcgcc
    11221 cgccatcgcg ggaaaatggc ttcacttcgg caccacggcc ggtttctact atgtgttgga
    11281 tcgcgacagc ggtgccgtgg tggccgagat cgactgcggt gagccgattt tcagcacgcc
    11341 ggtcgtggcg aaggatcgcg tgtatgccgc cacgctcggg gcgcaggtgc tggcgatgac
    11401 gtttgaaggc aagcagctct ggaagtggga tttcgtgaag gaagtcgtcg gcttcgacgg
    11461 caatcgctgg agcggcgagt cctgggccgc gttttgcgag aagcgcatgc tcgccacgct
    11521 caaaccgggc acgaagccaa cttccagcga tggccgcgtg acgtggaagg atcacttcgt
    11581 gtgctcgcgg gacatttgtt tgatcaaagg caacatcgtc gtcattcccg ccggtggtcg
    11641 cacgctgttc ctcgaagatt tgggcgaaac accgaagctc aaagccaccg gcgagattcc
    11701 cgagtggtcc ggcaaggaat tccccgccac cttcggccaa agcgccgatg acgaaggcaa
    11761 cgtctatgtc caatggcatc gccgcgacaa cgccggccgc gtggacatca tgcgcctcga
    11821 aggcgacacg ctcaaaaccg gcgacttcgt ccccggcacg gacaccaaca tccgcatgga
    11881 tggtttgctg agcttttcac ccgtggcgat tcgtgggaac gaggtcttcc gcgtgaagcc
    11941 agaggcgggg cggggattga tccgacacga catgaacaca aaggagttca aaactttcag
    12001 cactcgtggc gctatctctc ctccggttct caccaagaac tttgtcattc acggcacact
    12061 ggatgggcag attcttgtgt ttccgatcaa cctaaacaaa gaagagccaa agaaccctct
    12121 gaccagcatc attccatttc gctatgtgga gacatcagga gaggccatct cggctcccgt
    12181 agcgattgcc gactcccgaa tctacgttcc aagcgaggat ggccatcttt atgtatgcgg
    12241 cgagagtaaa gggaagagtc agccagtggt cagaggcctt gaagtttcta atttgcgctc
    12301 ccctctcacc ggcaagctcg ccgatgccaa atacgactgg ttcacgaact acggcaactt
    12361 cgcggggcag aactcgaaca gccaggacct caagccgccg ttgcgcatgc gctgggcgcg
    12421 tcgtctcgaa ggcacggtga agcatctgcc cgtctgcggc ggtggtcgca tgtacacgca
    12481 cacggcggag gggcagatca tcgcgtgcga gcaggacaca gggcgtctgc tgtggcggaa
    12541 gtggtggccg gatgtctatt tgagcttcac ctcgccgctc tacatcgacg gcaagctgct
    12601 ggtgccgcag gcaggcatca agaagtcctt cgtgcgttgt ctcgatgccg cgacgggcaa
    12661 catgctgtgg gaggcgccgt tcaccggttc gccaagctgg agccggcagt tcccgccggt
    12721 tgtcgcgggt aacgtcgcga tctatgcgag cggaagcggt gattatgcgc cgcaaggcag
    12781 tgaaaaaccg tggacctttg gcggtgaact cgtgaaaccc gcggatggac gcgaagtgat
    12841 gagctttatc tactcgaacg acaatccgta ttacccggcg aaccatcatc cgcgcctctg
    12901 ggcctgggat ttggacacgg gcaaggtggt gtgggagaag gactttttcg agttcgggcg
    12961 cggcggcaat gactgcggca tctgtatcct cgacggcaag ctctactaca gcgtcttttt
    13021 cggctacgac gccgacacga agcggcgtcg cggtctgccg gtcgaaaaca acggcctcac
    13081 ctgctgcatt gacccgcaga ccgggaacat tctctggcag acaaacgaat attacgtcac
    13141 cgcgaaatgc acgctcagcg cccgcgacgg caggcttttc atcggcggct acaaccgcgc
    13201 gcagcagggc accgacgacc gctttgtctg gtgccttgat gccaataccg gcaagctcgt
    13261 atggaaatcc gatgccgtga cctccgcgct caacgtggtg agcgtgggtg agcgcttcat
    13321 gttttccaac gccctgcgcg ggaagggcaa catctttgac cgcgagaccg gcaaggtcag
    13381 ctacagcatc ctcaccaact acgcctgctg ccgcttcacc atgagcgagc cctatgtcct
    13441 cggtgcgaac atggacatga ttgatctcag ccaggaaggc aagctcgtct ccaccggccc
    13501 cgccatcgac tcgcgcgaat gcctcggcgc cgtagtcagc aacggccgca tcttttacac
    13561 ctcgcaggcg agcggattcg ttgtttcgca gacctacggg ccggagtcga aggacctgcc
    13621 gccggcgtgg gaggcgcggt gaggctgccg cgcgtggttt tccattcaaa cgcggcattg
    13681 ccatgccggg cgtcctggtc taaaactggc atgttcgttc gtcttctgga ggtgcccgcc
    13741 tggtttgccc ggcaaacgca ggcggctttt tcaaagtctg ggcggcagcc aattcacgac
    13801 gctccaaccc ctgcgagcgt ggtgccggtc cccgtagttc aatggataga acaagggttt
    13861 cctaaacctt aaatgcaggt tcgattcctg ccggggacgc cactttgatt caacatttgg
    13921 ctcaaatgac gggctgccgg cgcgttttgt gggacagcca tgaaacgtca catcctcttt
    13981 attgtcctgt tttttgtttc ttccgccgct tccgccgcga actggcccat gtggcgcggc
    14041 gccaacggtg acggcacctg ctccgaatcc gatctgcctt tcaaatggag cgccacggaa
    14101 aatgtgctct ggaaaacgcg gctgcccgag ggcggcaact ccacgcccgt ggtgtggggt
    14161 gacaaagtct ttgtcaccca ggcggtggaa aaggacgggc gccgccttct gctgtgtttc
    14221 gacgccaaga cgggcaaaca aatctgggac gctggcacga tttaccgcga ggcggaattg
    14281 acccacaaaa ccaaccccta ctgcgccgcg agcccggcca cagatggcga gctggtggtc
    14341 gtctttttcg cttcggcggg tgttttttgc tatgacatga gcggcaagga gatttggaaa
    14401 cgcaccgatc tgggcaagca gcatcacatc tggggcaacg gcacttctcc tgtgatcgcg
    14461 ggcaatcatg tctttctgaa tttcggcccc ggcgaaaaca cggtccttta cgccttcgac
    14521 aaaaagacgg gcaaaaccct gtggcagcaa aaggagccgg gcgggaattc cggcgaggga
    14581 ggcggcaaaa actggctcgg ctcctggagt gatccgctgc tgcgcaaggt ggacgcgcgc
    14641 tatgaactca tcatgtcctg gcccggccgc gcctgcgctt atgatccgat gagcggcaga
    14701 gagctgtgga catgcgcggg attgaatcct ctggtttaca actcgcctct gtatgcggac
    14761 ggcctcgtgg tggcctttgg cggctatggc ggcatgggtc tggcggtgaa agccggcggc
    14821 gagggggatg tgacccagtc ccgcagggtg tggcacctgc ccaaggtcag ccagcgcatc
    14881 ggctccggtg tgctgcacga ggggcatcac tacatcctca gcgatggcgg catcgccgaa
    14941 tgccgggatt tgaaaacggg tgagatggtc tggagtgagc ggctcaaagg acctggaccc
    15001 accgggcaaa actggtcctc gctggtgctg acggctgaca agctgtgtta tgcggtcaat
    15061 caaggcgggg atgcttttgt cttccgcgcc agcccgaaat ttgagcttct agccacaaac
    15121 tccatcggtg agaaagtcat cggctccatc gctgtgagtg acgggaggct tttcattcgc
    15181 agctatcaga acctctggtg catcgggggc aggtaagggc ggcgtgtttg ccgacttcgc
    15241 tgcttgattg gctgccgcgg aggcgttgag ctatccgccg atgcgttacg ctttttttga
    15301 cctcgaccac acgctgctgc cattcgacac gcagacgttg ttttgcaatt atgtgctgca
    15361 ccggcatccg tggcgggtgg tgctgcattt gtggttcctg ccctttgcgc tggggcgcgc
    15421 gctcggcctg gtgagcacgg ccacggccaa gcgcgccttt ctttcctatc tctgggggat
    15481 gaaacgggcg catctgcgca ggctggcgcg ggagttcgcc agcgattgcg tggactcgtg
    15541 gatctaccct gagctgcgcg cggagatcct ccgccacaag catcagggcc ggaagctggt
    15601 gctgaacacg gccagtccgg atttttacgc ccatgagatc gctgaggtgc tggagttcga
    15661 ccactgcgtc agcacgcgct ttcacattgg caacgaggtg ccgctgcgcc cggtgctgga
    15721 gggccccaac aacaaacgcg aggccaagat cgccgccatg cgcgaggcca tccccggtgt
    15781 gcgcgccctc tctgacgagc agcgctttca ctgctgggcg tatagcgaca gcccggcgga
    15841 catcccgctg ctggagttcg ccgggaaccg cgtgctggtg catccgggcg gcaaactgcg
    15901 ccgtcacttc ccgcagcatg acgtgaccat cctcactccg cgccgtccct actgggggcg
    15961 gttgggagat ttgctggccg cgctgaggca aatgctgggc ctgcacggcg agcgcggtcc
    16021 gcgccggtga gaatccgccg ggccgggcgt gaaaaaacaa accggttgca ccccgggtgg
    16081 ggacggttat tctccgcgcc cattttccaa accaccaacg aacgtcatca tgtcccagca
    16141 tcgcagcctc aaaacctccg gctccgtcgg cagcaagcgc agtgtcctga aacgcggcga
    16201 gcgcatcaag ctgatgaagg cccgcggtca atggcaggaa ggccgtctgc cgaccggcct
    16261 gcccaagacc aagtccgaat aacccgcggc gctgttcctc cgggaacgcg cctttcagcc
    16321 cagcccgtgc gcctcaaccg ctatctcagc ttgtgcggac tcggttcgcg ccggggagtg
    16381 gagacactta tccttgaagg ccgggtctgc atcaacggcc attacatccg cgatctggcc
    16441 accgacgttt ccgacgagga caaggtcacc gtggacggca ggcccgcgag ggcggagacg
    16501 cccgtcgtgc tggcattgaa caagccgcgc ggctacatct gctcccgcag cgatgagcgc
    16561 gaccgcatga cgatttacgc gctgctgccc cgccagtacc agcatctgca tcacgcgggc
    16621 cgcctggaca aggagagcga ggggctgctg ctgcttacca accgcggcga tctttcccac
    16681 cggcttatcc accccagcca gggagcggag aaggaatacg aggtcgtcac cgacaagccg
    16741 atggaccttg agactatgaa caagctggtc cacggcatga tgaccgagga ggggcacgcc
    16801 aaggctgagc gcgcctggct ggccagcgag tacagggcgc atgtcgtgct caaacagggg
    16861 cttaagaggc agatccgcct gatgttttac cagctcggct tcgaagtgga gcggctgcgc
    16921 cgcatccgca tcggctggct cgaaatccgc ggactgccca aaggcggatg gcgcgagctt
    16981 tcggaggccg aagtggagcg ctttttttcc aaagaggggg ggactgggcg tgcctccacg
    17041 aacactgaca aaccggtgct caaacgccgc gcagcgcgga cggaggagcg cggcgcgaag
    17101 gacgagcggc ggaagccgaa attcgaagga cggaagcggg gcgaacgcaa gccggggaag
    17161 gccaggccgg caacgggcag gccggcgttt aaaaagcccg gggccaaggg gtcgcgcaag
    17221 cgcccgccgc gctgacccgg cgtgatcggg actgcgcgcg aaacccggat cgagggagat
    17281 tccgtccatg ctcccgcagg catctacacc atgaccgcca aaaaaatcac gcgctgaaca
    17341 cggcggcgcg tttgcctgcg gcgtccggcg cattcaagtt gggccatgca tgaaagcctc
    17401 agccaagtcc tcgcctcgga atccacacgc cccgcgctga cagtgcgcgg ctgggtgcgc
    17461 acgaaacggg agtcgaagtc atgcgcgttc ctggaggtga ccgacggctc gtgtttcaag
    17521 tccctgcagg tggtggtgga cgcggcgctg ccatcggccg cgctgctgcc gcgcatcctg
    17581 acaggggccg cggtcgaggt ggagggcgcc ctggtggact cgcccgcgcc cggccagcgc
    17641 tgggagatgc aggcgcggga gctgcgcctg gtgggcgggg cggacgcggc ttatccgctt
    17701 cagaaaaagg ggcacacacc ggagtttctg cgcaccatcg cgcacctgcg gccgcgcacg
    17761 aacttgtttg gcaatgtctt ccgggtgcgc agccgcatgt cttacgcggt gcatcgtttt
    17821 tttcaggagc ggggcttcat ccatgtccac acgccggtga tcacctcgaa tgattgcgag
    17881 ggcgccgggg agatgttcag ggtgacgacg ctcaagcccg gcggcaacgc ccgggcggag
    17941 gacgattttt tcggcaggcc cgcctacctc acggtcagcg gccagctcca gggggaggct
    18001 ttcgcctgcg cgctggggaa aatctacacc ttcggaccca ccttccgcgc ggagaacagc
    18061 aacacggcgc gtcatgcggc ggagttttgg atgatcgagc ctgagatggc cttttttgac
    18121 ctgtccgccg acatgacttt ggcggaggaa aacgtgcgct atctggtgaa agccatgctg
    18181 gatgagtgcg gtgaggagct ggagttcttc ggcagatttg tggacaagac gctggaggcc
    18241 cggctccggc agactctgga gaaacccttc gagcgcttca gctacacgga agcggtggat
    18301 ctgctgctga aatccggacg cgctttcgag caccccgtca tctggggcga ggggctgcag
    18361 acggagcacg agcgcttcat tgccgaggag catgtgcgcg ggccggtgac gattttcgat
    18421 tatccaaaat ccatcaagcc tttctacatg cgccagaatg acgacggcag gaccgtggcg
    18481 gccatggacc tcctggtgcc gggcatcggc gagatcatcg gcggcagcca gcgcgaggag
    18541 cgtctggacg tgcttcagga ggcgatgcgc gcgcaaggtc tcagtgagga ggactattcc
    18601 tggtatgtgg accttaggcg ctatggcagt gttccgcatg ctggctacgg catgggattc
    18661 gagcgcctgc tcatgtttgt caccggcgtg gccaatatcc gggatgtgat cccctttgca
    18721 cgcacaccgg ggcatgcgga gtattaaaac attaaagcgg ggcgcggatt tcagcgttcc
    18781 cagattcacg catgttttcc attcatccag ccggcgcctc atgcgcagcc tcccgtttcg
    18841 ccgggtgcgt gctgtcgtgc ctggccgcgt ccgttttgca ggctcagacg ggattgaaaa
    18901 tcgaactggt gccggagacc acggccatcg tgcccgggga ggcttttcgc gcgggcatcc
    18961 tcatccagcc ggagccgggc tggcatacct attggcggca gccgggcatc gtcggcgtgc
    19021 cgacgcggat ggcctgggag ctgccggagg gtttttccgc cggggagttg gagtatccgg
    19081 ggccggaggc agtgctgatg tttcacatca aggcgcaggg ttttaaaagg gacgtgcttt
    19141 tgcaaacgcg catcacagcg ccaggccacc tgaagccggg agaaactgtc acgctgcgcg
    19201 ggcgcgcctc ctggatgtgc tgcggcaaca tctgccatcc gggcactcaa attctggaat
    19261 taaaaatgcc cgtggccagc ggcgctgagc cgcatgagcg ctgggcaccc gttttcgagc
    19321 gcgaaagaga gacctttgag cgtgaaagct ccctctggga ggtagaggcg gaggaaaacg
    19381 gtatgacggc cacaatgaaa ttgcgacccg ctggcgctgg cgcgaggctt tttgacggag
    19441 aagccgggac agaggggctg cgcttcttca cggaagacgg ctggatcaat tccgacgagg
    19501 agcagacgtt caccctcacc aatgacggag cgctcaaagt ggggctgacc cgcgcggagg
    19561 tgttcctggg cggcggcggc ccgcccgagc ggcttcatgg ggtgctgcgc cgcgagggag
    19621 gctgggaaat ggggcaatcc tggtcctgcc tgcgcatcgc tcccaagatc acgcgccggg
    19681 atgcgttgaa agacttgcag acccgtcgtt gaatttccca agccgggcgc taacctccaa
    19741 agaaaccgtc atgaaaggca tcctcgttct catcctcgcc gcagcagtgg gctatgtggc
    19801 ctaccagcac gtctatccga tcatcgccga ggcgatgaaa ttcgagaaac accagcctca
    19861 aaagcaggag gtggccgtcg tggcgcccaa gcctgagaag ccggagatca aagtcgagcc
    19921 gcccaaaccc gcgccggagc cggaaatgaa gccggagccg gagatgaagc cggaacccaa
    19981 gatggtcatc cccgagcctc cgcccatgcc cgagccgccc aaaccgaaag aaggcgagtt
    20041 tgtcccgccg accttcgatc ccatcgaagt ggccacccaa aactggtcgg tcattcctgc
    20101 gaccgccttc ccgcgccagg tcaaaatgaa acgcgacctc aatcttcaaa tgaagatcgg
    20161 cgcatccaca gcttccaccc aggtgaaagc cggcggcagc atttatgccg tgggccagga
    20221 gggcagccag ctcatcgtct cgcccactga caaatccccc atgcgcggca aagtggcttt
    20281 cgaagacact gacctcaagg aggtgctcac cgatgtttac gagcgctgga aggtggcgcg
    20341 caccgagacg ctgcgccgcg cccatgagtt caagctggcc gccgccgaac gcgccaaaaa
    20401 cgccccggcc accgccacaa ccgccagcgc cagcaatgac aagcccgcgc gtgacgctga
    20461 cggcacctac ccgctgctca tagccagcat gaaatccggc caggtgaccg agatcaagcc
    20521 cgagagcatc aaagaatggg gcgagccgca gatgaccgac ggtgtctgga ctgtcatcgt
    20581 caaatacgag acgcagacca tgttcggcaa gttcgacacg gaggcgcagg catccatcaa
    20641 aaacggcaag gtggaaaagt ggatctacac aggatcggga gaagtggtgc cctgatccgg
    20701 cgcatgtatt ccagaggacg aaccgctttt gaattccctc cttaacaggc ttgaatcccg
    20761 cctcggcagg tttgccattc cgggactcgt gcagctcatc gccacgctgc agctcatcac
    20821 gctcgtggtc ctcatgttcc tgcccgtgga ggcgcgggaa ccgtatgagg cgctgctggt
    20881 gtttgaccgc gcgctcatcc tcaaaggcga ggcatggcgg ctgttcagct acgttttcat
    20941 ccccgtaccg aacctctttt tcgccttcat cgccttcatg ttcatgcgct tcctcggcgc
    21001 gggccttgat gagggctggg gggcgtttcg tgtcaatctg tatgttctgg gcggcatggc
    21061 ggcgctggcg gccggcgcgc tggtttttgg cttcagcgcc gatgccatgt gggtttatct
    21121 cgccgtcctc ttcgcctttg ccaccttgta cccgaacgag gaaatcctgc tctttttcat
    21181 cctgccggtg aagatcaaat gggttgccct tttctccgct gtcaccgtcg cgctgctggt
    21241 cttctcctcc ccggtcttcg cgatccccgc gttcttcgcg ctgctgaatt atctcatcgt
    21301 gttcacgccc ggattcgttc gcggcaggct ccatgccgcc cgcgtggccg ggcggcgcgc
    21361 ccgctttgag ggcgccgccg gtgacggcgc ttttttccac caatgcaaag tctgcggcaa
    21421 gaccgaggtg gacgaccccg ccctgcagtt ccgggtcaac gacgagggcg acgaaatttg
    21481 ctccgtctgc cgcgtcaaac catgacggtg caagccgcgc ttgccttcgc gcgcgccgat
    21541 gccagctttc ccgcccttca catatgcgct gccccaaatg cggaagctcc caggacaagg
    21601 tcatagactc gcgcgaggcg cgcgaggggc aggccatacg ccggcggcgc gagtgcatga
    21661 gctgcggttt ccgcttcacc acctacgaaa tcgtcgagcg cgaggagttg cgcgtggtca
    21721 agcgcaacgg cgcccgccag gccttcaacc gcgacaagct catgtccggc ctggtcaaag
    21781 cctgcgagaa gcgtcccgtc accatggagc agatcgagca gctcgccgac gacatcgcca
    21841 acgagctgga ggaggagggc taccgcgaga taccctcgac ggccatcggc gccaaggtca
    21901 tggccaggct cgaggagata gatcatgtgg cctacgtccg ctacgcctcc gtttaccgcc
    21961 agttcgaaga cgtgggcgag ttcattgacg agatccggaa cctggaccag cgccgccccc
    22021 ccgaccccaa tcaggccgtg cttttcccct gacaccgccc ctccgccatg cccgagaaaa
    22081 ccattttcca gaagatcata gaccgtgaaa tccccgcgcc actggtctat gaggacgacc
    22141 tcgtcgccgc cttcaacgac atcgcgccgc aggcgccggt gcatgtcttg atcgtgccca
    22201 aaaaactcat cccgcgcgtc ggcgaggctg cggcggagga ccaggctgct ctcggagccc
    22261 tgctgctcgc tgcgggcagg atcgccgaaa agctcggcat caatgacacc ggcaaaggct
    22321 tccgtctcgt catcaaccac ggcagggacg ctggcgaaag cgtgccccac ctccacgtcc
    22381 atctcctcgc aggccgccca atggagtggc cgccaggctg aggcgtgccg gggataaaaa
    22441 acctcccggc atcacgggga tgacgggagg tttgtgcgca aattggaagc ttacttgctg
    22501 ttttcggcga actgcgcgtc gaagatgatc ttcgagggcg ggaagtctat cttcttgcag
    22561 aacgcggcgg attccgtggc gccgaactcg cggtccatcg cgccgtcctc ccactccacg
    22621 gagagcgggc cgccgtattc gatgtcgttg agggcgcgga tgatgcgctc gaagttgatg
    22681 ttgccgcggc cgacggactt gaagtcccag tagcggctgg gtttgtggaa gtccacatgc
    22741 ccgccgaaga caccggtggt gccgtcgccg atgccccagg ccacgtcctt catgtggacg
    22801 tggaagatgc ggtctttgaa ctggtagatg aacttgatgt aatccacgcc ctggtagccg
    22861 aagtggctgg ggtcgtagtt gaagccgaag gcgggatggt aatcgagcgc ctggagcgcg
    22921 cgctcggcgc tggcgatgtc aaaggcgatc tcggtggggt ggacttcgag cgcgtagcgg
    22981 atgccaagct tctgatactc gtcgaggatg ggcttgaaac gcctggcgaa gtccgcgtag
    23041 ccggcgtcaa tctggcctgg caggttcggt gggaatgaat acacaaggtg ccagatgctg
    23101 ctgccggtga agccgttgac gacgctgacg ccgaagcgct tggctgcgtg cgcggtcttg
    23161 atcagctcct cggcggcgcg ctggcgcacg ccctcgggat ctccgtcacc atagatgtgc
    23221 ggcggcagga tctgggcgtg acgggggtca atgttgtcgc agacggcctg gccgacaagg
    23281 tgcgtggaga tggcgtggca ctgcatgccg gcctccttga gcttggcgcg cttggcgtcg
    23341 cagtaggcct ggtcggcctt gtccacctcg aagtggtcgc cccagcaggc aagctccagg
    23401 ccgtcgtagc cgaagttttt gcctttttgc agcatggttt caaaagaaag gtcggcccac
    23461 tggccggtga aaagagtgac gggtctgccc atggtggttg atgattctgt aaagggttga
    23521 tgttggtttt tcggagtcgc ggagacggcg ctgattttcg acatccgcgc gcgggtggca
    23581 atgagaaaaa tcagccggtg ctgcgccggt caatgcgtca gcgcgtagaa gatgatgttg
    23641 atgccaagcg ggtaggcgta cttttccgag aactcgcgga agtaccaggg gtccgtgccc
    23701 tcctcctccc agccgtcgcc caggtcggtg ttgtggcaga tgatcatgac catgcgcttt
    23761 ttgtcatcat agaccgcctt gtagtgcggc gtctgcgcgt cccagcgctc gaaggtgatg
    23821 ccctgggacc gcccggccat cgcgtggccc acgctgggga tctgcggctt cactttcaga
    23881 gggaagacca tgcggaaaat ttcatgctcc agtggcagct cctccggctc gcggtcggga
    23941 aagatttgct tcagggcaaa ataaaacgtg tcccattcct gctctcccca gaagtcgtcc
    24001 accatcatga agccgccgtt gagcaggtac tcgcgcagga tgcgcgcctc gtcttcggtg
    24061 aggctgatcc cgccgggttc gatcatgtag ataaaggggt agtgccggag ctgctccggc
    24121 acgatgtcca cgatggcccc cttcggactc acctgcagcg aggtgagctg ctggagacga
    24181 taggaaaagt tcaggtccgc gtccgggtag tccgtcgtcc agcgaccgcc acccccgcgc
    24241 cgcctgcccc aggattcgga attgtagcgc agccgcgcga agacgaagtg gtcgtccggc
    24301 gtctcacggc tcaccggcca ggtcgggaag tcggcaaagt atccgcgtcc gtattcgcgg
    24361 ggatcctggg ggaagtaatc gtccttgatg ccgaaattcg tcggagaacc ggagtcctgc
    24421 gccagcacga ccagggcgag aaggccaagg gccagtgcgg aaagtgggcg cttcatgagg
    24481 aaggtgattg caggatggat gccagccggg cgaaggatgg aacggctcgg ccgcgcgtct
    24541 atttcacggc acgcccagca cgcgctggat caggtcataa gacgcgagtc tttccttgtc
    24601 agggaaatca tgatcgcagt ccgggtggtg gatttccagc cgctccgccg cgccatgcag
    24661 ggcgaaaacc ggccgcgccg ccgcagcgat gcggtccacg ctgtcccatt tgaaattcga
    24721 atcgtgcagc ggcgcgctga caaagacatg ccttggcgcc agacaggcga tcagttcata
    24781 gtgatcgaag ggagcgtcct gcgggcggcc gaggtattcc gccattttta gaatgtaccg
    24841 ctcctgcacc cagccctgga tgttcccgtc cttgtagtcg agaagggagt cgaagccgca
    24901 gcttgtgacc accgccttga gccgctcatc gaagacggcg gtgaaaatgc tgttatgccc
    24961 gccgagcgag tggccgatgg tggcaaagcc ggcttctttt ttcacgaagg gcagcgtctc
    25021 cagcaggtcg agtccgcgga tgttgtccca caccgccttc atcgtgccgc tggtgaagcc
    25081 cagcgctttg aggtcaggct ggtattgcgc cagcagggga taactcggcg ccagagtcac
    25141 aaagccgcgc tccgccaact cggccgcata ttgccggttc ggcctgccgc cgaggccgac
    25201 cacgactttg tggccgatca cattgtccgt ggggtgcagg cacagcactg cgggcgccgc
    25261 cttgccggcc agcgcggctt tcggcacgca cagatacgcg gtcaccctgc cgccggggga
    25321 ggactgatag ctgatgagcc ggcgcacata gccgccgcag tcctcctcct cgtgcgtctg
    25381 aggctccagc gggcagcgtg cctccggccc gggcagaggg ccggtcattt gctgaaagcc
    25441 gtccagagcc tccttccggc gcgcttccca ttccgccgtg gtggaagctc ttttcaccgc
    25501 gccggatgcg tcgcgatact ccagcaagtc ggtccggctc aggccgcgca gcggggggat
    25561 ttcagcggac gtaagggcct cggcgctcgc gccgaggcag gccagaatgg caaagagttt
    25621 catctttccc gatacgacac ggcgcggaga ttccttcccg cgcatccatt cttgccaccc
    25681 gctttgtcgc gcgcagactg cgaccacaaa atgaacctca ccccatggac gaacctgctg
    25741 ctttttgacg gcgtctgcca cctttgcgac agcacggtgc agttcgtcct gcgacatgac
    25801 acatccgggc gcatccattt ctgcccgatt cagtcggaga ccgggtccag gttgtaccgc
    25861 gagcatggtt tcgaccccgc cgcgccgcac accatgctgc tgctgacacc ggacggcgct
    25921 ctgcgggaaa gcgatgccgc cattgagatc gcgcgcctgc tgggaggaat ctggcgtctc
    25981 gccctagtcc tcaaaatcat cccccgcccg ctgcgggatg cggcctaccg cttcatcgcc
    26041 agccgccgct accgttggtt cggccgtcat ggtgcctgcc tcgtgccgct tcctgaatgg
    26101 cgggcgcgtt ttttgcaatg accatcttcc ggcttgcgcc gcgcccttga gaggttacaa
    26161 atccagcccg ctcgcggtgt tgcgccgtga gatttccgca gatttcgcca gcaatggcaa
    26221 acggcgggct gtggctcagc ctggtagagc gcttcgttcg ggacgaagag gtcgtgggtt
    26281 cgaatcccgc cagcccgacc accttctaag gtggtcttat tcaaggagtt ccatagactt
    26341 gggaacttcc aagaagggcc cgatgccgcc ctcactccac cgctttgagt gactgaaaac
    26401 gactcgaaaa gccccaagtt ccaaacgtcc gggggggcag ggtgactacc cccggcataa
    26461 ctgccatgca gtagaagctg ggggatcaca cccacttttt gaattcgagc aatgaattgt
    26521 ggaacacctc acggtggctt gttattgcta ttctggtcat tttcgctgcg tggctgctct
    26581 caagaaatga gcgatcgtga ccaaacactc cacagctcag ttgagtttgc tggcatcagt
    26641 ccgcgccgag aacttcctgc aagccgagcc gctgcttttc aaagtccgcc tcatccagcc
    26701 ggcgcggaat ggtgacgacg ggttcgaaga cgaccttcac acgcgaaaac ggcagcggca
    26761 gcatgaagcg gtcccaggtc ttgaagcgca gcgcgcggca gtactcgaca cgcaccggaa
    26821 cgatgactcc gccggtgagc tgggccagct tcaaaatgcc gggctggagc cggtagcacg
    26881 gtccgcgcgg accgtccggc gtgatgccca cgtcacgccc ttccttgact ttttccgcca
    26941 gcatgatgag cgcgccgagt cccttctgcg gcttggagct ggagccgcgc gcggcctcga
    27001 atccgaactc gccgcaggcg tcggcgatga tctggccgtc gccgctgggg ctggaaagta
    27061 tggctccggg gatgtgcgcg aaccaccggt cgtgaatgta ggggatgaca aacatgcggt
    27121 tgtgccagaa tgcccagatc cagcccggac gcgcccggtc aaaggcgtcc gcgcggtcct
    27181 ccacctcgaa acggagagtg gccccgacgc accggatgag gagcgcgacg agtttgccta
    27241 ggccttggat gcggattttt gccatgtgca tgcatcctga aacggcacgc ccccggtttc
    27301 aacccgcaga gcgcgccaag tcccgccgct gccatcccgg ttcccgatgg acgcttggca
    27361 aaccctccgg cggctcctat gatccgccat gtccgccgcc cccgtcaccg tcctcattgc
    27421 cgacgaccac gccgtcgtgc gcaggggatt cgcgcggatt cttgacagcg gctgggattt
    27481 caaagtggtg gcagaggttt ccaacgggcg cgaggcggtg gcgtcagcgg aggagttgcg
    27541 tcccgatgtg gcggtgatgg atgtgaccat gcccgaactc aacggcattg aagcaacacg
    27601 caggcttctc aaagtctctc caaagacccg cgtgctggcg ctctcaatgc acaaggactc
    27661 cgtttatgtg cgcgaaatcc tgcgcgccgg agccaaggga tacctgctca aggacagcag
    27721 cgaggcggat ttcctcaagg ccatgcgcgc cgtgtcccag gggcagggct acctcagccc
    27781 ggaggtggct gacgccgtgc tcgacgacta ccgcaggcat gtcacccatc cgatagatct
    27841 gctctccagc cgcgaacgcg aggtgctgca attgatcgcc gagagcagga ccaacaaaga
    27901 gatcgccagc gcgctgcacc tcagcgttta caccgtcgag gcgcaccggg ggcgcatcat
    27961 ggaaaaactc aacctccaca gcatcggaga gctggtgcgc tttgccatgc gccacggcct
    28021 tgttgattga ggtgaacggt cagccgccat gagacgacga ctcgccccct tcacacggcg
    28081 catcacgcag cgcacggtgc tgcgcggtct tgtcacaggt ttcggactgg tggttctgct
    28141 gcttggcatg acaggactcc tggccgtgag tgaaagccgg gccatccgcc gcggagtcag
    28201 cggcatcgtg cgcgagcagc tcctcatcgc ccgtcttctc aacgaggtgc aggcggagga
    28261 gaacaccctc accgaggtgc tgcaccagat cacccgcaag tctcaaagtc tcgacaagga
    28321 agccttgctg caggatctcg ccgttgcgga cgatgccgtg acgcgcctcg cctccgacgc
    28381 cgccgctacc ggtaaggcgg aaatttgggg gcgcctggta tccagcgtgc gttccttctc
    28441 ctcgcaggcc cgagccgcgc tcgcaggcag ggacgccgtt tccgaagccg gcctcgaaag
    28501 cctctttgca aagcatgacg aagtggttgc tctcatccat ggactgctgc gtgacagcac
    28561 cgcccatctg cgcgaagcgg acgcccaaat cgaaaagcaa tcccgcgtgc tggcgcgcca
    28621 gtccgccctc ctgcttggct cctcgctgct gatggctgtg ctttgcgcgc tgggcaccat
    28681 acttttcgtg cgccacagcc tgcgccgaat cgaatggcag gcagaggaga tcaaccgcgt
    28741 ctcctggcac atgctgcagt cccaggagga gaccgcccgg cggttttccc acgagttgca
    28801 tgacgaactc ggccagtccc ttgccgctgt gcgcgccaac ctcacctcca aaagccacga
    28861 cgtctcgggt gcgcgaagtg ccgactgcgt tcagcttgtg gatgaggcga tcgccaacgt
    28921 gcgcgaactt tcccaactgc tccgtccggt aattctcgac gatttcggcc ttgatgcagg
    28981 tctgcgctgg ctggcggaaa agttcgccca gcgcacgcgg ctgaaggtgg actgcgattc
    29041 gacctgtccc ggccgttttg cagatgaaac cgagacgcat ctcttccgca tcgcgcagga
    29101 ggcgctgaca aatatcgccc ggcatgccga ggccacgcgt gtcgtcatcc agctagccct
    29161 tgaggacaaa aacatccgcc tgagcgtcgt tgacgatggc aagggcctcc ccgatgagga
    29221 atctaaagct gcgccaagcc ttggcatggt cggcatgcgc gcccgcgcgc ggcagtgtgg
    29281 cggcgaactc agtgtcagcc ccgctccgcc ccacggcctg cgcattgagg ccgtggtgcc
    29341 gctgagatga caggcggatt gaccggatca atgcgcggcg ttcgcggcgg cgacggtgcg
    29401 ctggtcgagc gtcttgaggg cctgcatctg cttgatggtg cgctccatgc cttcgttgga
    29461 gccgtcgaag aacatgacgt tgcctttgcc gtgctccgag atgtatttca tgccgtcgag
    29521 ccacatggtg aagagcatga agccgggagg cacatcgccg gcctgcatgg ttttgccggc
    29581 gttggcgaga ccttcggcga cttgttcgcg gaagcgggcg tttccggcac ctttgagctc
    29641 ggaggcttct ttgtcggcct cggctttgac gcggatggcg cgggcttcgg cctcggcggc
    29701 gcgggttttg atgaggtatt gggcctgcgc ctcgttttcg gcggccatct tcatgttgtt
    29761 gctggaaacg acctgcgaca tggagcgcat gatggcttca tcaaaggaga tgtcgttgat
    29821 ctggaggtcg aggaggtgga agccccaact gttgagtgct tcttcgagat ggtccttcac
    29881 cgcgccgatg atttcggagc gcagcacgag gatttcggcc tgttttttgt tcgccacata
    29941 ggagcggatg ctgccctcca cgctgcggac gagggtctgc atgaagctct tttcgtcgat
    30001 gaagcggaag gcgatttttt tgatggtctc ctcaccagca tcctgcgcgg agtacacgat
    30061 gagagatttg aagttcacgt aggcctgatc aagcgagatg gcttcgaatt cgagttcgat
    30121 ggactggttc tgcgttgaga tgctcttgaa gacgccctcg atgagcggga gcttgaagtt
    30181 gaggccggga agcatcaggc gctggtattt gccgaagcgg gtgacgaccc cgacgtggcc
    30241 ctgcttgatc acagaggcgc tgaggatgac gagaacgatg aggatgacgg cggcgggtat
    30301 catgacgtgg cttggtttgt tcgttggtaa aggcggtgag agaaaatcag gcggggaact
    30361 cggaggagtg gtgctcgagg atgaaccagc cggtcttttc ctttttgcag acgaaggagt
    30421 agcgggcggg cacgctgatg agttccttct tgcgcttgaa ggtgaagacg tatttgccgg
    30481 actggatgta gatgtccttg tactggcgca cctcgccgct ctcgaacctc acatgcacgt
    30541 cctccagctc gaagaggtgc tcgaagtagc ctttgatctt gtgatggccc tcacgcagct
    30601 ccttggcgaa ggtgccccag agcaggccgt tcatgtcgta gcagccgacg acggcggcca
    30661 tgctgtgatt gttgatgcca aaggcccagt cgttgatgaa ctcggtcagg tttttcttct
    30721 ccgccgcatt gtggctgatg gtgctgtagc gcaggccgcc gacctcgtgg tcgcgatgca
    30781 cgagcatggt gtccgtgctg cgaggatcga tctcgtcgcc gaatttgtgg gcgatttttt
    30841 tcagcatctc gatccagggc atgtgctcgt ggcggaagat gacgaagtgg atgaagagat
    30901 ggatgcccag cacgacgatg agcccctcga agatgccgac ggtgtaggtg aggatgccgc
    30961 agaagagcgc cagcagcagc gcatagctgc catgcgcggc cacatgcgtg aggccgacga
    31021 tcatcttcca cccggtgaag atcatgagca cggcgagcga gaaatacggc agatgctcca
    31081 gcagatgtgg atcgagaatg aagacgagga taacgcccgc ggtgaagagg ttcgatatct
    31141 tcgtgacggc accggcgagg gcgttcgtgg cgcttttcgc gagtccgtcg aggttcgtca
    31201 tgccaccgaa gaagctgctg ccgatgttgg ccacccagat ggcgagcagg ctgttgtttg
    31261 tgtttgccgg acggcccagt ggatcgagtt tttcgatggc ggcgttgctc atcacctgct
    31321 cgatcacgtc gatgagcgcc agcatgagcg cgtagccgat gatctcggcg accatctttg
    31381 gcgggatgac atcgaaatcg ggaaacggcc acgcgacttt gatcggcgag tgctcgatgt
    31441 gcagcatcgg gatgtgcatg tagatgccgg aaatgacgcc gacgagcacc acggccacat
    31501 agggcacggc gggatacttt gccttgtact tgccgaagag atgcaaaaac agtcccagcg
    31561 ccccgagcga gagtgcgatc atcttgattc gctccatggt gaaccagtca tggctctcgc
    31621 cgaggatgcc gtaggtgtaa ggcaggaatt tgagcgcgat tttgagtccg atgcccgcca
    31681 gcaggccctc aatgaggtaa ctcgggatcg ctttgagaat gaagcgctgg aggttgaact
    31741 tccatgcaat ggcctgcatc accgacgtgc aaaagatgag gaagggcatg ttctccatgc
    31801 cgaatttatg cacactcatc gccagcaccg gcgcgagtcc tgccgccacg ccaggcacgc
    31861 cgacgtagtt ccccggacgg aagaggaagg tcacaaatcc tatcaaacac gcgaaaagca
    31921 cggtgagcag tcccgtctcg atgggatact ccgacatgag gcagatgccg accgtgagcg
    31981 gcaccgcgat gaggcccgtg atggaacccg cgagcgcatc acggcgcaga ttttcgagcg
    32041 tgaagatggc gggcctctcc gccttctccg caatgagcag atcgctggcg agcggcttct
    32101 tccggcggcg cggatgcgga tggtaatcga ccgccgaggc agtggtcgga tggatgagcg
    32161 caggttcggc ggcgtttgac atggcccgga ataacccgcg cccgcctccg ctatgccatc
    32221 cccccggcag gggattccca ggccccttga cacaggagag gcatgctatt tgagaggagt
    32281 cagcctgaaa ttcttgaact gaacgaccat cggcggacct tggtgaattt gcagggcaag
    32341 caagccctct ttcggggcgt tggccgcgtc ctcatcagtc acatccacgg tctgcatgcc
    32401 gttgatgaaa tgctgaatgt ggtttccttt ggcgatgatt ttgtagtcgt tccagtcctc
    32461 ttttttgatg gactgctgga tggcctcgct gtcaccgact gtgccggctt tttcaacggc
    32521 aggcttcttg ggatcctgcc ccggcttgat gacggtcttc tctccgcgaa gggccagaat
    32581 tccgcggcct ttctcctcgt agagaatgcc gctgtatttg tcaccggcct cgaagtccgc
    32641 ctgatagccg ctgatgacgg gcccgaactc cccgggcgcc agttccttgc tgcggtactg
    32701 cacgccggag ttgccctttt caatacggta ttggaactca agctcgaaat cggacacggt
    32761 gccgcctttc cagacgagga atgtgttgtg cttgatgatt cctttctcag gattggccgg
    32821 gtcaggcggc gtgatgccgg tgatggcgcc gtctttgaca ctccagagat ccaagttgcc
    32881 ctcccagccg gccaggtctt ttccgttgaa aacctcaacg gtgcttgagg aggctggccc
    32941 ggatcctcca gagggagcgg agggcttgca ggagggcaat gcgaacgcgg cgagcacgca
    33001 aagggagaag aatgccgggg ttttcatggt tggtgagtgc cgctttctaa tgcggtggca
    33061 tgactttgtc caccacctct gctcaccgcc ctttgcggcg gtgctggcgc gcgcggaatt
    33121 cggcggggcc gcagccggtg tgccggctga aaacacgggt gagataatgc cggttgggga
    33181 agccggattc gagggcgatt tgatccacgg ttttgtcggt caatgccagg agctgctggg
    33241 catggcgcac gcgcgcggcg ctgacgtagg cggcgggagt gctgccggtg tgctcgcgga
    33301 aggcgcggat gaaacgctcc aggctcatgc ccgcgcgggc agcgagcagg cggtttgaca
    33361 ggtcggcatg aggagcgcgc tggacgaggg acaacacccc gagcaaccgc tccggcatgg
    33421 gtggtggcgc gggcaggctc agccgggcga aggcggcgta aagcaacgcg ctgctttcat
    33481 gaagcaggcg gtggtcgcgc gtgtcggagg cgtgctcatg atgcagggcg atgacgcttt
    33541 gcgccagagt cgtcaaaagg gcatccatcg gcagcagaag cggcgcgggg tccacggggc
    33601 cgccgggacg gttcacggtg aagtgcatcc acagatgaca cgcgggcacc gggccgcggc
    33661 agtcaaagac cgtgtccgcc gggatgatca tgcaggtgga gggttcgagg gcgatctgcc
    33721 tgccgcgaaa atgcacaaaa cagcccggct tggggttgtg ataaaaacgc caaaagggac
    33781 tgtccacacc gtcgtgattc caatcctcca gcgccgcttg ataaccgcat tcatgcagca
    33841 aaaagtccga ccagcccgcg ccggagacgc gcgcgaggct gacgccccag ggcgtggtgt
    33901 agttcaggta tttgcgccgc gtgttcacga tccgacacaa acggagcaga atatggaatg
    33961 ccgtccaggc gttcttcaca gcatgagaaa atgcgttttc cctctgctat cgaaccgcct
    34021 tgtaatgatg tggcgcgatg gcttgcgttg gagttcaagc tttagcttgt tctgggaaag
    34081 ctccgcagcg gagtccactc ccgtggcgcc taaaggcttc actccaacag gcctcactcc
    34141 taccgttgga gttcaagctt tagcttgtgc ggggagatct tcgcagcgga gtcctctccc
    34201 gtgtcgccta aaggcttcac tccaacgggc ggggctgctt gccttgatgg tcgtgcctgc
    34261 attcacccag ggcgcggaca agctgcgctt caatcgcgac atccgcccca tcctttcgga
    34321 caagtgtttt cattgccacg gcttcgatcc aaaaacgcgc aaggccggcc tgcgtctcga
    34381 caccttcgag ggcgcgacgg aggatggtgg catcgtggcc gggcgggcgg agcgcagcgg
    34441 gatcatcgag cgcgtgctca gtcatgaccc ggatgaggcc atgccgccgc cggagtcaaa
    34501 gatcggccgg ctttccgaca cggagatcgc gactttgaaa cgctggatca atgaaggtgc
    34561 cgagtatgag gcgcactggg cctttgtgcc gctggacaaa gcgcagctcg atgaatggac
    34621 acgccaggcc aaggcggcgg gcgtggcatc gccgatggac tggatcgtta gcgaggggtt
    34681 gaaggcgcac ggcctggcga tgcaaccgcg cgcggatgcg gccacgctgg cgcggcgcgt
    34741 cagctttgat ctcacgggcc tgccgccatc ggtggaggag ctggcagatg tggagcttga
    34801 gtcactcatc acgaaaaagc tcgcctcacc cgcgtatggt gagcgcatgg cggcggactg
    34861 gctcgacatc gcgcggtatg cggacagctt cggctttcag gtggaccgcg agcgcgaggt
    34921 gtggatgtgg cgggactggg tggtgcgcgc cttcaatgac aacctgccct ttgatcaatt
    34981 catcacctgg caactcgcgg gagatctgct gccgcaggtc accgatgagc aggtactggc
    35041 caccgctttc aatcgcctgc atcagcagga aagcgagggc ggttccgtgg aggaggaata
    35101 ccgcatcgag tatgtggcgg accgggtgca aaccgtggcc accgcgtttc tcggcctcac
    35161 cttcgagtgc gcgcgctgcc atgaccacaa gtatgatccc gtgagccagc gcgagtatta
    35221 cggcatggca tccttcttcc aaaacattga cgaggcgggt ctgtattcgt tcttcacctc
    35281 cgcgccgccg acacccgcca tgatgctgat ggacgcgcct gcaaaggaga agatgaccgc
    35341 gctcaaggcg cgcgtggccg ctctggaaaa gcagctcgcc agcacagggg tgaagccgct
    35401 cgctgagatc ccgctgcgcg atggcgtgcc ggtttccttg gaagggcaac ttgctcattt
    35461 cagtttcgac gcactggagg gcaacaaact cgccgattcc ctcaaccccg agtcgcctcc
    35521 cgcgcctgcg ccgaagaaag cggacccgaa gaccaaagac aaagcgccgc ctggtccgtc
    35581 cgccattttg aaaggtgaga acaagctcgt gcagggtcgt cttggacagg ccgtgcagtt
    35641 cacgggggac gatccggttg acacccctgt tgggaatttc cgccgccatg agccttttag
    35701 cgtttcgctc tggatgaaga cgccagatgt gaaggagcgc gcggttgttt ttcaccgctc
    35761 acgcgcgtgg acggatgcgg cgagccgggg ctacgaactg ctcattgagg agggtcgctt
    35821 gaagtggtcg cttattcatt tctggccggg caatgccatc tccatccgcg cgtgtgatcc
    35881 gctgccggtg aatgagtggg cgcacgtcat cgtcacgaat gacggcagca gccgagcctc
    35941 aggactgcgg cttttcatca atggcaaagc ggtgccggtg gacgtgatcc gcgacggcct
    36001 cactcaagac atcaccggcg gtggaggcga taccatctca ctcggcgagc gcttccggga
    36061 tcgtggcttc aaaggcgggc tcattgacga ctttcgcgtc tttcagcgcg acctctccat
    36121 gccagtgagg ccggagctgg agccactgct ggccgagctc aaagccgcgc gcggcgagct
    36181 gaccgacttc tccggacagc aaaaagagat catggtcatg cgcgagctgc ccgagccaaa
    36241 gaaggcctat gtgctcacgc gcggcgagta tgacaagccc acggaggagg tcacggccat
    36301 gacgcccgcc tcgctgccgc cctttccgca ggacgcgcca aagaaccggc tcggactcgc
    36361 gcgctggctg accgatcctg gccatcctct cacggcgcgc gtgacggtga accgcgtgtg
    36421 gcagagtttg ttcgggcgcg gtctggtgaa gacgagcgag gacttcggca gccaggggga
    36481 aaagccgctt tatcccgagc tgctcgatta tctttcgctg cgcctcattc acagcggctg
    36541 ggacatgaag gcgctgctgc gcgagatcgt ctgcagcgcg gtctatcagc agcgcagcat
    36601 cgctgaaaaa caggtgatgg aggatgaccc ggagaatcaa tggctggcgc gcgggccggc
    36661 attccggctg cctgccgaga tgatccgcga caatgcgctg gccgccgccg ggctgctgaa
    36721 tccgaagatg ggcggcccac cggtcaaccc ttatgaaatg gccgaggcct ttaaaccttt
    36781 ccaggtggcc aaagaaggcg agtcgcttta tcggcgcagt ctttacacca cctggcggcg
    36841 caccagcccg ccgcccgcca tgctggcctt tgacgcgccg cgccgcgcgg tgtgcagcgc
    36901 caagcgcgag cgcaccgaga gccccttgca ggcgctcatc ctgctgaatg gcacgcagtt
    36961 cgtggaggcc gcgcgcgtgc ttggggagaa tctgcaccac gagcacggcg gcgcggtcga
    37021 gaaaatgatc cgccacggct tcctgcgctg cctgagccga cagccggaca cgcgcgagat
    37081 ggaaatctgc accaggttat accaagagca gctcgcttac tttcgcgcac atctgcagga
    37141 agcagtggag ctgatgaaaa ccggtggcgc gccgagagtg gcaggcgtgc cggatgtcga
    37201 aggcgcagca gccgccgcgc tggcgcaggc tttgctgaac catgatgctt gtgtggttaa
    37261 gcggtgaggt gtggaaccac taatgaacac tcatgagcac taatggatgg gcttgaaatg
    37321 ggggatgccg ccggggagga tggccgcctt caaccacaaa ccacacgata tgagaataca
    37381 taaaaatcat cgaatcgaaa tgcgggagga tggacctttt cagcaggagg gatacgacct
    37441 gatggctgcc gcgtttgaag tgcataacga gaatggcggc gggctggcgg aagaggttta
    37501 tcaagagagt ttcgagatcg aactggagat gcggggcatg ctttttgtgg cgaaacagga
    37561 actggcgttg ttttacaaag ggcgtgaact gaagcgccgc tacatcccgg atctttatgt
    37621 gtgcgaaggt atcgtcgtgg aactcaagtc cgtggctcaa ctcctgccgg aacattcggc
    37681 ccagctcttt aactacatgc gcatcagcgg caaaagtgtc ggctatctca tcaacttcgc
    37741 tcccatcaag cgcatcgaat ggcggcgtta cgtcctgtga aacccaaaat cattagtgct
    37801 tattagcgtc cattagtggt tccaaaaatc tcatccgaga gaagcgctcc acgaacatga
    37861 tggacaaacc catcccatac ccccacctgt ctatgaaacc caaatcttca gattccataa
    37921 gagttcccag cgctttcctc aaccgccggg catggctcca gaagtcaggc atgggcctgg
    37981 gaggtctggc tttggcggag cttttgatgc gggagcaggc gggggcttct ccggtggatg
    38041 cgggcgtgct gggcggcacg cactggcagc cgaaggcgaa gcgcatcatt taccttttca
    38101 tgtccggcgg cccctcgcat ctggacctgt atgattacaa gccgctgctg aatcaacgcc
    38161 acggcgagca actaccggac agcgtgcgcg gcgggcagag gctgacgggc atgtcgggga
    38221 atcagtcgtc catcccgatg gtgggctcgc ctttcaagtt cacccggcac gggcaggcgg
    38281 gcgcgtggtt cagcgagctg ctgccgcaca cggcctccat cgcggatgac ctgtgcgtgg
    38341 tgcgctccat gttcacggaa agcatcaacc acgggccggg cgtgacgttt ttccagactg
    38401 gctcgcagat cgccgggcgg ccgagcatgg gcgcgtggtt gagctatggt ctcggcgcgg
    38461 agaatgcgaa cctgccctct ttcaccgtgc tcatcaccaa ggacaagggc ggccagccgc
    38521 tgggcgcgca tctgtggggc agcggcttcc tgccgacgaa gcaccagggc gtgctcttcc
    38581 gcgcggcgaa ggaccccgtg ctgtatctcg gcaatccggc gggggtgagc gcggacagcc
    38641 gccggctcat gctcgaccgc ctgcgcgagc tgcacgagca tcaatatgcg ggcacgccgg
    38701 atgtggagat ccagaaccgc atggaccact atgagatggc ctggcgcatg caaagcagca
    38761 ttccggaggc caccgacatc ggcgatgagc cgcagcatgt gctcgacatg tatgggccgg
    38821 atgtgacgaa gcccggcacc ttcgccgcga actgcctgca ggcgcgccgc ctggctgaga
    38881 gaggcgtgcg cttcatccag ctctaccatc aggactggga ccaccatggc ggtctgcccg
    38941 gcgcgctgcc gcgtctatgc aaagaaaccg accaggcctc cgccgcgctg gtgaaggatc
    39001 tcaagcagcg cggcctgctg gaggacacgc tcgtggtctg gggcggcgag tttggccgca
    39061 cgaactactg ccaggggaaa atccaggcca acttcggacg cgaccatcac ccgcgctgct
    39121 tcagcgtctg gatggccggc ggcggtgtga agccgggcgc ggtctatgga gagacggatg
    39181 agtttggcta caacgtcgcg cgcgacggcg tccacatcca cgacttccat gccacgctca
    39241 tgcacctgct cgggatagat cacgagcgcc tgacctacaa gttccagggc cgccgctacc
    39301 gcctcacgga tgtgcatggt catgtggtga aggggctgct ggcttagctc ccggcgaaaa
    39361 acttcccgcc atccgcgttc tctgcccatg cccaagactt tgctcgccat cggcgcccac
    39421 tatgatgact gccctttcgg cattcccggc atcctgctca aagccgtgcg cgagcatcac
    39481 cgcgtggtca tcctcagcct catcggagat tacaccaact ggccgcccgt gcaggggcgc
    39541 gagcaggcgc tgctggacaa gaccgccgcc atggccgccg agcgcggcat ggaaatgcgc
    39601 ttcctgaagc acgccagcat gggctttgag ccgactctgg cgacgaagcg cgaggtggcc
    39661 gagatcgtcg ccgaagtgcg gcccgacacc gccttcatgc tctggcggca ggaccgccac
    39721 ccggaccacg aggccgcctc cgccatctgc cacgactcgc ttttccagcc cgcgcgcctg
    39781 acgggcaatg ccaacgcgcg cagcccttcg caggtcttct ggtatgacaa cggccccggc
    39841 cacacgatag acttcacgcc cgacacttat gtggatgtca gcgccgagtg gtccggcgtg
    39901 gtggagtggc tgggcggctt gatggccttc gtgcgcaacg gaccctttga tccgcaaaag
    39961 gatgccgccg tggaggcgaa gacggtgctg gcgcgctacc gcggcctggc ctgcggggtg
    40021 aaacatgccg aggctctgcg cagcgtccgc ccgctgtcgc gcgaggtttg gtgagggtgc
    40081 gaccgggaag cttgcatcgc ccagctcaag ccaggatgtc tttcaccaca tgcccgtgga
    40141 tgtcggtgag gcggtagtcg cggccttgga agcggtgcgt gaggcgctcg tggtcaaagc
    40201 cgagcagatg catcacggtg gcctggaagt catgcacatg gaccttgtgc tcagccaccg
    40261 caaagcccat ctcgtcggtg gctccataat cgaatcccgc cttcacacca ccgccggcca
    40321 tgaacagcgt gaagcagtca ggataatgat cacggcctag aaccttgctg gcggcggtgc
    40381 ggccctcgcg gaagggtgtg cggccaaatt caccgcccca gatgacgagg gtgtcatcga
    40441 gcagcccgcg ctgcttgaga tccttgatga gcgcggccac gggcttgtct gtctcggcca
    40501 tctttttcgt caggccgtcg gtgatgcctg tggcctcgct agtgccgtgg aaatcccagc
    40561 cccagtcaaa gagatggaca aagcgcacgc cctgctccac gagccggcgc gccagcaggc
    40621 agttgttggc aaagctggcc gcgccgggct ttgccccgta ttcctcgatg acccgcgcgg
    40681 gctcgcgcga gatgtccatc acctcgggca cgctggcctg catcctgtag gccagctcgt
    40741 attgctcgat gcgtgtgcgc gtctcgggat ggaccagctc ggcggcctgg cgctcgttga
    40801 gctcgcgcag cacatcaagc gtcttccggc gcagcgcgcg gtccatgcct ggaggattgt
    40861 tgacgaaaag cacggggtca ccctgggacc ggcactgcac accctgatag actgagggga
    40921 tgaacccgct gccccagcag ctttggccgc cggagggctg ggtgccgctg gagatgagca
    40981 cgacaaagcc cggcagatcc tgattttccg tgcccagccc gtaagtggcc catgaaccca
    41041 tcgaggggcg gccctggcgg gtgtggccgg tgaacagcag cagctcggcg ggcgcgtggt
    41101 tgaactggtc cgtgctcatc gagcggatga gcgtgatctc gtccgccagg gtgtggaagt
    41161 ttgggcatgc gtcactcatc cacatgcccg ccctgccatg gcgcgcgaac ttccgcggcg
    41221 tgcccatgag cttcggcacg cccgatgtga aagcgaaagt gcggcctttc aaaaacgagt
    41281 ccgggcagtc ctgaccgtcg cgcctcacca gctccggctt gtagtcaaag aggtccagat
    41341 gcggtggcgc gccggacatg tgcaggtaga cgacgcgctt cgcctttgct gtgtggtgcg
    41401 gatgccgggc ggagagcggc cccggcgcgg cccgcgcgcc tgagtcgaag gcatgcaggg
    41461 cgatggcgcc caggctgaac tggcccgcgc cggtcaaaaa ttggcgccgg gtgcgcaggt
    41521 gaagggactc gaaatgcggg gacatgctcc gggtagtacg ttggggggat tcaaaaactc
    41581 acggctttgc ggctgccggg aatcaaaggc agctgggatg gtccgcggat cgaggatggg
    41641 caggcgtccc ggttgcccgc atctgaaagt gcaatcttga gggttgcgcc accttacctg
    41701 ccccaggccc accggaaaag attccggctg tcggtgaagc gggcctcgtc cgaggtggat
    41761 cccagtgtga cgacgatgag gccgcgcccg ttgtggctgc ccgtggccac caggcagcgc
    41821 ccggcgctcg gcgtggtgcc ggttttcacg ccgtcgtagt tggcgagctg gagcagctcg
    41881 ttggtgttcc tccactccgc gtcacgctcg ccgccatcgg gcaggcggac cttgccggtg
    41941 cgggcggccg tggcgacgac ctcgcggaaa agcgcgtgct tcctcgccgc caaagtcagc
    42001 gtgaccagat cgcgcgcggt ggtggtgtag tcattggcct cgccgccgtc cccgtaggaa
    42061 atgcggtaag tggtggagtt catgcccagg cggcgcgcgg tgcggttcat ctcggcgatg
    42121 aagttcgcgc gtgtcgcgta atcgggagtc ttcacgcgct ccggagtctc atctcccggc
    42181 ggctggagac gcgggtggaa atgctccgcg aaggcgttgc ccatgtcatt gcccgagggc
    42241 agcatgaggg cgaaaaggcc gtcgcgcacg cgcacctgct cgccctcctt gagttcggcg
    42301 gcggagccct gggtgctggc ggcgagcttc gtgacggcga cccattcatc gagaacggcg
    42361 gggtctttga gcgcgagttc cagcacgaca aaggcgcaca tcgttttcgt cgtgctggcg
    42421 gccttgcgcg gctcgtcagg cagcagcgac tgcaaaaccg cgccgctttc gagatccgcg
    42481 atgacccagg atttcgccgt cacgggcgga ggggattcca ggttctctga gggaagatcg
    42541 gaggcttgag cggcggccag ggacatgatg gaaaggcagc agagggtgcg cgcggcggag
    42601 tcgttcatga tgaggcaaag gaacggatct tatcagggct tcttcacgga ggaaccgccc
    42661 gccgggcggt gctcacgcca gattttcaac acctcgtcca ccggcatggc cttgaccgcg
    42721 cgtccgcggt gccccgtcat gtcggcggcc atgaacagag agttcaagat ggcctcctcg
    42781 gcagcctcga tgacggccag gaacagcgga gtcatggcct cgttccgcat cagcgcggca
    42841 ggcggattca gcggaccctc atgcttgtgc gggatgcgca gctcccttgc ggtggagaag
    42901 gcgatgacat aatccccgct gccattcgcg gcgatgccgc ccgtgcgtcc cagccccagc
    42961 atcgcgcgcg cggccagccg ccgcagatta cgcgcgtcca gcggcgcgtc cgtggccacc
    43021 acgatcatgc aactgccatc cgcgccgtca taggcttccc gcatgaaatg gcggccgagg
    43081 accttgtcca ccggcgcgcc gtcgagcgtg aacacgccgc cgaagttgct ctgcgccagc
    43141 acgccgaccg tgtggccgcc gtcacgcgcc ggcagcacgc gcgaggccgt gccaatgccg
    43201 cccttccagc caaaggcc
//
LOCUS       DAOMYJ010000017        42476 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Prosthecobacter sp. isolate AMR_MDS_5629
            NODE_349_length_42476_cov_20.046675, whole genome shotgun sequence.
ACCESSION   DAOMYJ010000017 DAOMYJ010000000
VERSION     DAOMYJ010000017.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867850
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prosthecobacter sp. (wastewater metagenome)
  ORGANISM  Prosthecobacter sp.
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Verrucomicrobiaceae; Prosthecobacter.
REFERENCE   1  (bases 1 to 42476)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 42476)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.30
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:34:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,742
            CDSs (total)                      :: 4,684
            Genes (coding)                    :: 4,676
            CDSs (with protein)               :: 4,676
            Genes (RNA)                       :: 58
            tRNAs                             :: 55
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..42476
                     /organism="Prosthecobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_349_length_42476_cov_20.046675"
                     /isolate="AMR_MDS_5629"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1965333"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..479)
                     /locus_tag="PLP58_03220"
     CDS             complement(<1..479)
                     /locus_tag="PLP58_03220"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HRJ07565.1"
                     /translation="MRARLACCLLLAGHSAPCQEPPVMRLDFPTANRALLGDKPEAFF
                     MGVDRGGSRVWQGGSFGFTRSPLLHEGGEIFTQFHEGTDIAPVERAADGTPLDEVRAM
                     ADGTVVFREERGGSGYGNQVLLRHDWDCGPVFTRYAHLSSVAVAPGERVRRGQKLGVL
                     "
     gene            complement(538..1056)
                     /locus_tag="PLP58_03225"
     CDS             complement(538..1056)
                     /locus_tag="PLP58_03225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009957979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome C"
                     /protein_id="HRJ07566.1"
                     /translation="MPIYEFYCPDNHKLYQFLARTAAQRGVVPRCPDNPAFKLQKQVS
                     RFAIIGRAKEDDGQDPFAGLDDEKMEAMMSGLEGDMGALDEENPDPRQLGRLMRRMTD
                     FMGGKTPPMLLEMARRLEAGEDPEKLEGEFGGMGGEGEALFAEMKKMIRHACGPARDP
                     KLYEMSDWLPES"
     gene            complement(1102..2505)
                     /locus_tag="PLP58_03230"
     CDS             complement(1102..2505)
                     /locus_tag="PLP58_03230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009960096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HRJ07567.1"
                     /translation="MRLHCPRFLGYEVGVSDDLFSPADTAPAQVEAAPDAPLASRMRP
                     RSLAEYAGQGHILGEGKLLRRAVEADRFSSLILYGPPGVGKTTLAHIISLETKARFVT
                     LSGVESSVADIRKEAEAAARLRKLNGQGTVLFVDEIHRFNKAQQDVLLPHLERGTLRF
                     IGATTHNPFFYVNSPLVSRSQVFTLEPLDVEALESLMARALADAERGLGGRGAVLTPE
                     AARHLATVADGDARKCLNALELAVLSTPPGADGRVLIDLAAAEESVQQKTVVYDGDGD
                     AHYDTASAFIKSMRASDPDAAVYWLAKMLHAGEDIRFIARRIVIAASEDVGLADSNAL
                     RVAVAAQQAVEVIGMPEARIILSHAVIYIATAPKSNRAYAAINAALEDVKNARTLPVP
                     KHLRDSHYKGAKQFGHGEGYLYPHDFEGGFVPQRCLEGGRVYYEPTTNGLEGRIKERL
                     DHYRRQWEAAANAGGEA"
     gene            2618..3229
                     /locus_tag="PLP58_03235"
     CDS             2618..3229
                     /locus_tag="PLP58_03235"
                     /inference="COORDINATES: protein motif:HMM:NF020965.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyloxyacyl hydrolase"
                     /protein_id="HRJ07568.1"
                     /translation="MKHPLPILACLVTSALHAGDPPLSPTVAPVPDTPWYERHALDFE
                     TGWLGKVGGSTPLDYSSVPVMLSWRSPEVLGLHFDGGSALIFRNRLTLLGQWFETGAE
                     NHYFGVMGAPSFEYWSAGGQWSLYLQVGGGAGWLDSQGVRGGQGQDFTLNWFAASGMS
                     YTLRESVQVRGGLMFQHLSNGGATDPNPGLDSLGFTIGLSWGF"
     gene            3335..5431
                     /locus_tag="PLP58_03240"
     CDS             3335..5431
                     /locus_tag="PLP58_03240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ07569.1"
                     /translation="MSEKFFPMPSSLEPLEQKIAPAGTVILSTAGGVLTITGDASDNG
                     IGITHVPSTGMWTITDPLAGTSYILNNGAPQAGGFNIPAQSAIVANLGDNNDRLDISP
                     SGTPSGLVLKALTINMGNGNDVIVMGTVSAQNLQVTGATTINLGEGNDTLNTTQSATY
                     GGLVKILGGGGNDTVNISGASGEQVFLKGLNVDLGTGNDNFNANVARFSVAGGSLVVK
                     NTGTAGGASSFNINSGLAIITVPTVFSTSLADLSVNLGNNMADVLHFGSTVSVIGGNG
                     TDAVNVNSQMTATSTVTFDLKNGANTTTLVTDGSLTGTSLVVKGGTGDDDLALQDSHD
                     LLVTGQLNFSAGNGTSTFIADVNSTLLAGSLVLNGGTGIDIFSFGGTSLNVMGSSTFN
                     MGAGANNNVQLAGTASSFIGGSLLVNGSDGTDQIVLDSPQFTILGSINTKFGNGTNVL
                     LAEGGSVYIGGGVNFSGGSGSDVLQAQSTSLIINKSTVFNTGAGGNTLYYRPDSGTVG
                     PVTYNGGSGTDTFALGNVDGTSTTRLSVNGAVTTNFGAGTFTSYYTDTIVHGIVNHKA
                     GALAGENENIIISESTFNSAVNILLGAGNADVDINDVFVRGAFTLDTGAGNDQVNVDT
                     LGGSSAFSSWFGMVKILTGAGDDIVVIGSSPVVVANAGNNFFSGLLVDGGAGGGDSFT
                     QGNNVFVGTNNQVNFP"
     gene            complement(5503..7941)
                     /gene="purL"
                     /locus_tag="PLP58_03245"
     CDS             complement(5503..7941)
                     /gene="purL"
                     /locus_tag="PLP58_03245"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009960551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurL"
                     /protein_id="HRJ07570.1"
                     /translation="MRSPLHSLQADLSSAPRTERAIYRTIPVRDLDGDALLALSKQMK
                     LSLSRADMEAVQKLYVEEGREPTDVELEVIAQTWSEHCKHRIFNARIEHTLNGVPEVV
                     DSLFKTYIRATTERIMEQKPDFVLSAFVDNAGFVKLDDDKAVCLKVETHNHPSAIEPY
                     AGANTGLGGVIRDILGAGKGAKPVASLDVFCFGPPDTKQEDLKAKDVIHPLGVMRGVV
                     RGVRDYGNRMGIPTVNGAIQFDDSFIYNPLVFCGTAGVIPIKDIAKEVKPGHLLIAAG
                     GRTGKDGLKGATFSSAELTTDSHEEDQTAVQIGNPIEEKKVADFVLAAREAGLIEFVT
                     DCGAGGFSSAAGEMLSEVGGEVWLENCPLKEPGLESWQVFISESQERMVMAVEEQNLP
                     ALQKLADTWQSEMFVLAKADGGKRLKVWHHGHAVCDLPVEKLHGAPRREMRAHWRQPE
                     AKTPKISVRPESWTQVLKTLLADFAIVSREPIIREYDHEVQGNTILKPLAGASGDAPQ
                     DGSVIAVDGSRQLVALACALLPEWGKADPHAMGRACVDECVRQLVAMGANPRRIAILD
                     NFCMGNPDNERELGALVECTKGMAQAALAYGAPFVSGKDSFYNYFITDEGPVSIPVTL
                     LVSGFGVIEDASHVVGASLRRAGSKLCLLGKTTVGLRGSVFAKHAQGAGLNGAPAWDD
                     AEAWASYGRYYELVKKGAILAAHDLSEGGLAVALAEMAFSGKAGIKIELENVPADKAC
                     TTAEVLFGETPGRLLIEVAPEHVEGVKLAGGVIIGESTGERWLHITQRGTTLIDSSIA
                     DLKPLWKEGLARWY"
     gene            8101..12156
                     /locus_tag="PLP58_03250"
     CDS             8101..12156
                     /locus_tag="PLP58_03250"
                     /inference="COORDINATES: protein motif:HMM:NF024753.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HRJ07571.1"
                     /translation="MNRLLLPLCLLAAPLQADWPTYLHDASRVGHSPENLATPLQTRW
                     TYSSSVAPDLAWPGEDGREIEGMVLENRIRFDDVFHTAIAGGRVYFGSSVDGRLYCQD
                     LATGKELWAFFTDGPIRLAPMIANGKIYVGSDDGHAYCLDAATGALVWRLRAGPDDER
                     VLARGRMISRWPIRTGVLVDDGVAFFGAGVFPHEKVFVHAVEAATGKVIWKNDTISQQ
                     DAGRSDLSPQGYLLATKDLLFVPSGRALAMAFDRHTGKPVNKPSPGWRGDAGGQIGGS
                     QAMLVDDQILAVGEHQVLALDQKTGKTGYAWFRGTQMTFSGSTGFMANGKEITAIDRD
                     AHARSTQERHDLEMKAYKLNGDLRKHPALAELKAVTAAEKVLKEAKASGGDAAKAEAA
                     LAKAAQKYEPLRVDYETKKAQLAGYRKRLEEFKEAGIKWSKPIPHESALILAGDTLVI
                     GGRNEVLCLNAATGEQTWKTAVEGEARGLAAADGHLLVSTTTGKVYAFADAALSLPQL
                     AQTPSLEMNPFPQDDLTTLYADAAEQALRQSGVTDGYCLVIGSEQGRLAWEIAQRSTL
                     KVYGLEPDAEKVRAARETLARTGLYGSRIVIDHLDLSVIPHASYFANLIVSDHLLLTG
                     KMPGLPQEVARVLKPIGGVMALGLPASAPADRQAAAKASVAEWIKATGLLEEKARLES
                     KDSLTLLTRAALPGADSWSHQYGNAANTSSNKDERIKGGLSVLWYGDPGPGKMVNRHV
                     GAVGPISTNGRLFIQGEWTVMAYDAFNGRFLWEVENPGALRHGLKGAYEPGNMAASDD
                     HLFVAIEDHCLQFDAATGRIVHQFKLPAQDLQPDKQWGYLAYEDGILYGAVTDRKELA
                     AVRARRTKAAAQASDAIFAYEVASQKLLWRHQGQYISHVTIAIGDGRVFFVDASLTPD
                     QRDDLLNADKTELAKLTGKARELAEERAKAADVRMATALDARTGVKAWAQPVDVTDCS
                     EIGAGGGSLTLIYHNGHLVLGGANANGHYWEQYLAGEFERRRLVVLDAEKGGKLWAKD
                     ANYKSRPTVINNEIIAEPWGYDLYTGAPKTRQHPLTGEDTPWLFSRPGHHCGAISATA
                     HLMFYRSKTTAFYNLDADEGTEHFGGQRLGCWINTIPANGLVMVPEASAGCVCLFSIA
                     STLVFEPREQSRMKWGVYGAAGDTRPVRHMALNLGAPGDRRDAHGKLWLGYPRPSSRT
                     GIDLPLKLTHTFAKDGAFYDVNEETLAIAGTDTPWLYASGAKGLSKLEIPLLNPGDAS
                     QTYEVRLHFTAQEDQRPFTIKLQGKPVATALDLAATPAARIETFTGIPVTETLTVEIE
                     PTTKEGTLPTLHALEILRTGEKEIKAGVAAR"
     gene            12211..13536
                     /locus_tag="PLP58_03255"
     CDS             12211..13536
                     /locus_tag="PLP58_03255"
                     /inference="COORDINATES: protein motif:HMM:NF024753.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-like beta-propeller repeat protein"
                     /protein_id="HRJ07572.1"
                     /translation="MDRFQILNPAASLRWAVVAALLSFPAEAWSDDWPQFRGLKRDNV
                     WRETGLLNSIPPEGLKIRWRQPSGPGWSSPAVAEGRVFLTDAELAKPDARERVRCFDA
                     VTGEVLWSYAYDVAYPEWAFVPGQGGGPTPTPVVEAGRVFTVGANSRVHCLDAATGAV
                     FWDYDPRQKYQVRDMQCRGSPLIDGDRLIAFIETKPGACVIALDKKSGKEIWRAVDER
                     VSNSSPLIVEAGGERQLIVWTDDSVTSLDPVTGEPWWREPMTTSNNDSVPTPVFDKNR
                     LLISGVMFELSTSEPTATMLWPGKDVAAPKRLLTNTGSPCLRGDHIYSAKLNGELVCL
                     AAKTGEIIWRDTTITSRKGGAAIHLIPIDDASAFFLFTDQGNLIRAQLTPKGYQELGR
                     ALLIKPTTPFAGRDCAWAPPAFANRHVFVRNDNEILCASLAAEETAPRQ"
     gene            complement(13780..14262)
                     /locus_tag="PLP58_03260"
     CDS             complement(13780..14262)
                     /locus_tag="PLP58_03260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ07573.1"
                     /translation="MRRKHYFPYRVSERPAWFHNLATQLLVVNPVLGVDTTTLNARVA
                     DALYCEYLCGPWLTWARGNGETATAAVETLYRGEGGGAFDPPVFTPPPLPGALPDALA
                     GLHGPPPTSSAQQPPDVHDTLWAISRVHAMENHEWTPMSTNFGGIMSGELKRREITPG
                     "
     gene            complement(15551..15922)
                     /locus_tag="PLP58_03265"
     CDS             complement(15551..15922)
                     /locus_tag="PLP58_03265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ07574.1"
                     /translation="MRLPALAFLVLALSGCGRGVIKHWEDGNYRVYTRPASEEIIMGH
                     YMGDGAILGMSDPTVISAGSDSRYVVFTRQLTSGGIEHYYIAKKVSGEGDVSGPFTPD
                     AYKAIATKLALPEFSWHLKQP"
     gene            complement(15960..16667)
                     /locus_tag="PLP58_03270"
     CDS             complement(15960..16667)
                     /locus_tag="PLP58_03270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ07575.1"
                     /translation="MAVERQTANQAGQIQPSPQGRMEERGALTTRLLPRKYPMIARRV
                     HGLAEAQGASSGGGGLFFSCGALGTGGSLAMMKMSHLSYPALVLAGVHRSVGVLKARQ
                     AVRVSRVVQGFASSSFMPRINHSRAGKASEKARMPQPAGIRLPRLRMSPSETQAGVPG
                     VRGGNRVTPMSRCPGLASCVEESHMDFSPVFARKAAWMLIAARMIHKSSVMNRKRRHK
                     ATNPSEIKLLAKLHFCE"
     gene            16725..17345
                     /locus_tag="PLP58_03275"
     CDS             16725..17345
                     /locus_tag="PLP58_03275"
                     /EC_number="2.5.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018969170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HRJ07576.1"
                     /translation="MFTGLVETTGQVIALETRGESARLTLDAGPLAAELALGESLAVN
                     GCCLTVAELDARSGRAGFDLLMQTLKVTSLGDLQPGSLVNLERAMALGARFGGHFVQG
                     HVDATARILDWSRHGQDHRLEVELPPGHRGLVIPKGSICLDGISLTAAEVGGDSVTCW
                     IIPHTRAVTHLRGKKAGDRVNVEFDMLGKYVRELMRAGSPAAGAAS"
     gene            17435..19462
                     /locus_tag="PLP58_03280"
     CDS             17435..19462
                     /locus_tag="PLP58_03280"
                     /inference="COORDINATES: protein motif:HMM:NF013100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HRJ07577.1"
                     /translation="MLRLACCLSLTAAGLGAQEPQETPKALPVESLGTMPEADAVKKK
                     RDLSASLLTQKEARTMRLSIPAPRGQIVDRNGIALATNKVVNYLALNFPFMEKATPEK
                     ILDYAKERINAVNQLLGKNWSLPDERLLTHYEHRRWLPLVFSIEEGLNVEITSEQQAK
                     LKLMLEEGSLLLQPAYLRHYPHEDAACHIVGYTGKTRPLPQGPIVDGDPLFEEMEGRE
                     GLEVTFDHFLKGVPGEINLLFDPNGKLLAEEVLRRPMPGHTLVTTLDFNLQKYAENAL
                     KKTARSGGSMVIMDVRNGDILAMASNPGFNLNEFIPGIRSARYEELANDPTMPLFARS
                     FRGEYPPASTFKIVTALGALESGRVSAATSFPCTVSMLVGDRYFHNWNTKNDEGPMNV
                     ITALKRSCNTWFYAAALQAGADPVTNMAFRLGFGERTGIPLKAEGRGNVPSSADHRIL
                     GGELANIAIGQGAVLVTPLQVCQSMAALGDGQRVFQPRLVKQVQTINEIVVDATEEKK
                     VRRELMLNPEFRETILKGMVAVVSGSGGTGRSAAIPQAQVAGKTGTAQWRVAKDQRLA
                     WFTGFLPASQPVLAFAVVYEGRPGEKIGGGAVAAPIVKEVFTKFYEGAPTDDPLVAAM
                     KDVPLALAVDEGVMDDVPLAQQVRPAETAPPPQTEQRTLGGFFRRLFKRSN"
     gene            19480..19905
                     /locus_tag="PLP58_03285"
     CDS             19480..19905
                     /locus_tag="PLP58_03285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ07578.1"
                     /translation="MTVQLATLCDSASDYSGKLCILGAFDTLCAREFPVVHPHCALVV
                     RLIFEPRDAGRHTFTIRCLDPSGQDCLPPFEPVVDVAFPSQFLPFVTRNIVLNLQRLR
                     IEKQGVYRWNIETGGITLASIPLRATLFEDPRGAAGPAG"
     gene            19931..20407
                     /locus_tag="PLP58_03290"
     CDS             19931..20407
                     /locus_tag="PLP58_03290"
                     /inference="COORDINATES: protein motif:HMM:NF040320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ07579.1"
                     /translation="MDSPVLQEMPVKAPAGHAIRQLSVFLQNRVGSLLALIRLLREHS
                     IEVLGLSMQDTTEMTLVRLVLSDPDGAGMLFIEKGIPHTDCPIIVVELGESGPGLAEC
                     LSALLTAELNIEFCYPLLVRPGLHPLLALHCDDCDIGAEVLHNAGFRVHSQGDLSR"
     gene            complement(20463..21368)
                     /gene="sucD"
                     /locus_tag="PLP58_03295"
     CDS             complement(20463..21368)
                     /gene="sucD"
                     /locus_tag="PLP58_03295"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007415627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate--CoA ligase subunit alpha"
                     /protein_id="HRJ07580.1"
                     /translation="MAILVDTDTKILVQGITGDFGSRHAKASLDYGTQLVAGVTPGKG
                     GQTFDHGGHKVPVFDTVGQAARETGATVSAIFVPPPFAADAILEAVDAGLDLVVAITE
                     GIPVNDMIKVKAAMQGSRTRLIGPNCPGVVTPGRGEKSQGGCRIGIAPGYIHKRGNVG
                     VVSRSGTLTYEAVWQLTTRGYGQSTCVGIGGDPVNGTNHLDVLKMFNDDPETEAIIMI
                     GEIGGNAEVEAARWAKENCKKPIAAFIAGATAPPGRRMGHAGAIIGGADDTAAAKKRI
                     LAECGISVADSPADMAEALFKVWGC"
     gene            complement(21478..21909)
                     /gene="hisI"
                     /locus_tag="PLP58_03300"
     CDS             complement(21478..21909)
                     /gene="hisI"
                     /locus_tag="PLP58_03300"
                     /EC_number="3.5.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012419663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004635 - phosphoribosyl-AMP
                     cyclohydrolase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyl-AMP cyclohydrolase"
                     /protein_id="HRJ07581.1"
                     /translation="MSEEKKYTFATRDSKESVEQGGGFAPKFDEHGLIPAMAIDARTD
                     EPLMLAYMNEESLKMTFEKGQAVYYSRSRKQLWHKGATTGEFQEIVEILTDCDQDALV
                     LRVIQHGGGCCHTKRPTCFYRRVVAPKDGGQLRLKMREFAP"
     gene            22087..22740
                     /gene="tmk"
                     /locus_tag="PLP58_03305"
     CDS             22087..22740
                     /gene="tmk"
                     /locus_tag="PLP58_03305"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007417436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HRJ07582.1"
                     /translation="MTAPAADPPGFFLSFEGSEGCGKSTQIRLLRARLEAAGRRVEVL
                     REPGGTEIGEEVRHLLQHAQEGRGMCAETELLLFAASRAQIVREKVRPLLAEGVFVIL
                     DRFLDSTTVYQGRARGLPLESVRAVNAFATGGTLPLLTVLLDMDARAAWSRIHETGRE
                     LDRMESQPLEFFEKVRAGYLALAGAEPERIRVVDADRAPETVHEEIWSLVQTRCHAL"
     gene            22730..23722
                     /locus_tag="PLP58_03310"
     CDS             22730..23722
                     /locus_tag="PLP58_03310"
                     /inference="COORDINATES: protein motif:HMM:NF024575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ07583.1"
                     /translation="MPFKADKALDLLARAHQHGRLGHAYLITGPRGADLMDFALRVLR
                     LVTGRERHHFEDFQREGLYILRPEGKSRRIKVGDDSSDPNTMRHFIHHFQMRSESTLK
                     AGVIAEAERMNDQAQNAFLRTLEEPPAGTLFLLLTHNPKALLTTTRSRVIEIPLLPPE
                     GVRVFTAYEQRLLELLENLTVRSSGSLGAALALKTEFQDILEALRGEIERDHKEAFDK
                     EKDHYKQTTDGSWLKAREDQVDAAIQAEYLGQRGALLDLLLAWMGDVARQQVGGEHLD
                     LDVFREATAKLAQRWTPEETDRRLRVLRQLESHLHTNVNESLALEVAFIQAFGR"
     gene            complement(23700..24041)
                     /locus_tag="PLP58_03315"
     CDS             complement(23700..24041)
                     /locus_tag="PLP58_03315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ07584.1"
                     /translation="MPWAKELELLAVNRLLDPRSELFVHEKWFPQTVMDVLLACDFAV
                     AHKDLLYRCLDREERRRPMSRCLKHCRHNETSKRIEWECNRDKWQAAERRDGAHLLRA
                     HWLIFTFRRPG"
     gene            complement(24811..27300)
                     /locus_tag="PLP58_03320"
     CDS             complement(24811..27300)
                     /locus_tag="PLP58_03320"
                     /inference="COORDINATES: protein motif:HMM:NF038032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CehA/McbA family metallohydrolase"
                     /protein_id="HRJ07585.1"
                     /translation="MKTAALFCLLPLSMHATEVFEARGDREKDLPGGKEADGIRGDFV
                     LRSDKVEALISHNAPNRRANMSTFYGDDGVSPGLLYDLTLRGQANDQLVAYGPCGHGP
                     VSYVRPAVTREEGAAAVESVITSALSGGVFKRHEYRVKDGVQGVFITTVLRNESGAPQ
                     KVSTKDDLTRFDSTGTTADGIRWADAINPAHKCGYAFGVLSLTGIEKLTDSLELAPGQ
                     EVVIARFFAVGTSPAQAVGEFRAVKGGALGTVSGQITGADGKGIPTASILVPEGKASV
                     PAYPDTEGKFSLKLPVGPAGLTITDLGRPDAQISLTVSEGATTAPKVAMQDAAGIHFA
                     ITGEDGSHLPCKAHFEPLDEGAPKLNLGPKWRAHGCVDQYHSENGHFTQQLPPGKYRV
                     VVVRGPEYGHIAREVSVEAGKITDFQGTLKRLVDTTGWISSDFHNHSTPSGDNVCDTD
                     GRLINIAAEHLEFAPTTEHNRFFDWEPTIQALGLAPFIKTVKGVELTGRRQHFNAFPF
                     EPDPLLQDGGAPVWNDDPRITALTLRRWQGERADRWIQFNHPDLSNMFVDRDGDGIAD
                     GGFVGVGEMIDGSESQNGPGTSILADSPFSLSRSGVATRVSYVREFLWRQLLNQGHRL
                     TAVGVADAHSVYGNGVGSWRIYLPSSTDDPAKIDWAELAPRAKAGNIILTTGPFLQVT
                     TPDGKTAGDDTRATGGMDLHVRVQCTDWMDINRVQVLVNSRPHPGFNFTRETHPQMFQ
                     NGVVKFDHKLRIPLQKDAHLIVVALHETGDMRTGFGTSDYAKMRPCAYNNPIYVDVDG
                     GGFQPNGDTLGFEIPGMGVTADKARALLGGE"
     gene            complement(27345..27977)
                     /locus_tag="PLP58_03325"
     CDS             complement(27345..27977)
                     /locus_tag="PLP58_03325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HRJ07586.1"
                     /translation="MKTWIFIVGGCLLAAVVFWSFQAAGPDEAQAASPPVPETKSLPP
                     GASGGANPGNPVAPSPNTPAPATVPMDAANEVILQEIEQASITYDAKDLPRIRPFLSH
                     PDPEIREAAINGMIVLGDAAAAPMLREASQIAPTPQEAVRMQEVAAYLELPSGSLVSR
                     TKDGRAVLGNPNRMPPQVKERYPGDYRKKSARPPQQPNAAETPASPATKE"
     gene            complement(28041..28958)
                     /locus_tag="PLP58_03330"
     CDS             complement(28041..28958)
                     /locus_tag="PLP58_03330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ07587.1"
                     /translation="MKALPRLAILGLLFGACSVTLAQVPGTYGAFTAALTLTHSKPGT
                     VLKDPETGKPLPVLDEFGDPLGGPTYFNDWFISKSTPAGDPISDEAHSEFISKMATRK
                     YSNKEILLDLVAAGAIEEIGNAPFIAGWSLIVVTDEFDDFSVLYARHTSGKVVDITPL
                     IQLPFGEDPTLGGYAQDVNFKYVNKMVFKPEDIIETETVTDVYKYKEKVSFIWMGDTL
                     IGHGILTGGGKLTFINIKDFDGTSFKYPVYVPGASKVSAISGIFTLPNVFDPATLNPV
                     SGVIEGTLSAAGGKVIPDIDAFLFPPVDP"
     gene            complement(29125..30291)
                     /locus_tag="PLP58_03335"
     CDS             complement(29125..30291)
                     /locus_tag="PLP58_03335"
                     /inference="COORDINATES: protein motif:HMM:NF027716.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5009 domain-containing protein"
                     /protein_id="HRJ07588.1"
                     /translation="MQPPPDRLVSLDAFRGFIMLLMASSGLGLAQMAKTEPDSRLWQE
                     IGWQVSHVDWTGCALWDLIQPSFMFMVGMALPFSLMRRKEQGQGFFGRFGHAIARALA
                     LVLIGVFLATRDGDAQTNWQFTNVLAQIGLGYVFLWLLAVCGWEYCAAAVVVILAGVW
                     WWFVQHPLPGADFDFASVGAAATDLLPGASGHWSKHLNAAADFDRWFLNLFPRKEPFV
                     TNAGGYTTLNFIPALATMLMGVVTAELLLRSRQTHARKCVNLLVAGVLCLLIGTLLDI
                     FLVPSVKRIWTPSWVIFSGGWVLMMLAAFYWLVEVAGQRRLVFPLVVVGMNSIFIYLM
                     HSLGAGWITGRLKTHIGAQWFSGEWAPVLERGGMLLVLWLLCLWLYRQRAFLRL"
     gene            complement(30337..31560)
                     /locus_tag="PLP58_03340"
     CDS             complement(30337..31560)
                     /locus_tag="PLP58_03340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009959810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AGE family epimerase/isomerase"
                     /protein_id="HRJ07589.1"
                     /translation="MIPCPLLTEPEGRARLSAFYRSELVENVMPFWLRHGMDREHDGI
                     FTCLDRDGSLLDSDKSVWFQGRAGWMFATLYNTVEKRRDWIEAAGSCIAFLDKHGHAT
                     NGKLFFTLTREGLPLRMRRYAYSETFACIARAAYARATGDHRYHEAAVRDFASFLRLA
                     FEPGGMSPKINEQTRPMISIGPPMIGIATAQELRANLGHVEVAGRSATEWIDKFIFDI
                     GRLFFKEDMDVLMETVAPDGGIIDHFDGRCLNPGHAIECAWFILHEGRLRGERAYIRL
                     GLRILDCMWRRGWDAEHGGFFYYRDVFDKPVAEYWQDMKFWWPHCEAVIAAALAHEIT
                     GEPKYAEMHRQAHEWSFSRFPDPEHGEWYGWLRRDGTPSTRMKGSLWKGPFHLPRMLW
                     YCSALLAAPPAAARL"
     gene            32398..34158
                     /locus_tag="PLP58_03345"
     CDS             32398..34158
                     /locus_tag="PLP58_03345"
                     /inference="COORDINATES: protein motif:HMM:NF015131.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c peroxidase"
                     /protein_id="HRJ07590.1"
                     /translation="MSGAIQRIWLPLLASLLPALSAGAQVLRLEVAPVWEKSELKSNH
                     WQPEGSPAGLSVNRLDFLLSKLALRRADGSWLESEDWHRFLSLEKGLLTAEAEGLPKE
                     DFTGIRFQVGVPPKENAGDAAQWPPAHALNPEVCGLHWGWQGGYVFMALEGRWRRADG
                     QLGGFSYHIANDENLTTVEMPVDFRGGGPVTIRLALDAAVLLGGVDFLRDGDSTHSRA
                     RDPLLVKFTGNLARAFRAQSVSYDLFQSPAVTEKAAPPPPGTTPFPLAVTQRFPQAAL
                     PADNPLTKEGVELGKRLFHDTRLSINNTQSCAGCHSRANALTDPRRVSLGAQGQAGKR
                     NAMPLFNLAWAREFFWDGRAKTLREQVLMPIQDAHEMNETLDNVVAKLADEGAEFDRA
                     FAPGGVTTERLAKALEQFLLSQISQNSRFDRAARKVAELTQEEKRGLQLFVTEYDPAR
                     GLFGADCFHCHGGTLFTDHQFKNNGLDLEPADRGRMLVTGDAADKGKFKTPSLRNIAV
                     TAPYMHDGRFATLEEVVEHYSGPMPRSDTLDPNLAKHPDAGIQLSAADKRALVAFLKT
                     LTDDDFIATPDGEVTQTNAP"
     gene            34266..>42476
                     /locus_tag="PLP58_03350"
     CDS             34266..>42476
                     /locus_tag="PLP58_03350"
                     /inference="COORDINATES: protein motif:HMM:NF012366.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HRJ07591.1"
                     /translation="MSIRGFLLPFYLTALLAHPAQADDEHQIDEQLLAEANALAPEFQ
                     AAQVEAPAPHELSLQGQWGPVIPWTPHIPVTAATLPDGRLLTFSSNQRTTFPSGVEFT
                     YAAVWNPATGAFTEINNNRHDMFCGGTVMLPDGRVLINGGRNTTRLGSIFDWRTNQWS
                     AIPNMNDGRWYNTSVALADGSVFTVTGDGGRTTAERWTASNGWARLGGINWSVVVNQP
                     GYVSNWHPLVLVAPDGRLFHGGPTRQMNWVTATGTGGLSYAGVDVPGALYPKEGCFAM
                     YDEGRILVAGGSSTTTANSFDSSTGTSTNAAFTLDIRAGAPVVQMTASMRYARQFVNS
                     VILPNGEVIAIGGNTSGRKFNDTGSILIPEIWNPQTGQWREVAAMSVPRNYHSLALLL
                     PNGRVWSGGGGLSGNSADHRDAQIYTPPQLFAADGAPAVRPVISQKPAYIAPGSVFQV
                     TASTGVARFSFIKMSSQTHSVNTDLRFLNVPFTGTAPGTYALTAHSNENVLTPGYWML
                     FALDAQDAWSQAAIMLVDPNLGVTITNPGAQISAVGVAASLTVLASAPGGGGTVSFSA
                     AGLPPGLSISAQGVISGTASQAGEFNVSVTAADGSTTASAEFVWSVTPANLYQNIADF
                     SGTDAMLQLNGVAALNGPVLRLTPNAGGQAGAAFLKSALPVSANTSFTTRFVFRMSGT
                     ADGADGMAFVVQGDRATALGNSGSGIGYESVLKSLAVEFDSYAGSGDPNGNHVGVLTN
                     GNVTSHLVTHTPAFDLEDGSSNTAWVEYDGVADTLRVFLAQGVVNSRPASPVITRAGL
                     DLPALTGGVAWVGFSAGTGGLANNHDVLAWEFNSNAFTLPQPPVLTNPGDQTGVVGMA
                     LSLPVQASDVNLDTLAYSATNLPSGLTINAATGVISGTPATAGAGIVTVTVSDGNTAP
                     VSADFEWLINPGLELQPVITQPAPAGTSITYTAQSTGGANPRYKWSFGDGTIETAFSA
                     NPAATHTFANPGRYLATLTATDDTGRVVTRDFWQNIHAPLTAERPAVSSAIACEPRAT
                     GNARVWVVNPDNDSVAVLDAVTRAKLAETPVGKAPRSVAVAPDGRVWVANVEDATLSI
                     LDSSTFAVAQTVTLARGSRPFGLAFAPDGSAAFVACESAGLLLKLHPTTGAQTGSCNV
                     GAHARHVSVAADSARVYVSRFITPPLPQENTASPQTSGHGGEVVVVNASAMTVERTLL
                     LAHSEAADSPTSARGIPNYLGAPAISPDGLSAWVPSKQDNIRRGMFREGSQLNHDQSV
                     RAIASRLNLATQTEDLPARVDFDDAGMPSAAVFDPFGNLLFVALESNRAVAVVDAWSN
                     EELLRFETGLAPQGLALSPDGGTLFVQNFMSRSVTVHDVAGLLAGSGAAPALLGTVGT
                     VATEALPAQVLLGKQHFYDAADTRLALQNYISCAACHNDGGHDGRVWDLTGFGEGLRN
                     TITLRGHGQHGALHWTGNFDEVHDFEGQIRGLAGGTGLMTDAQFNTGTRNLPLGDPKA
                     GVSADLDALAAYVTSLTSESKSPHRASNGALTAQGAEGEKVFRRENCASCHAGENFTN
                     SALGVFRDVGTLKPSSGQRLGAALTGLDVPTLRGVWATAPYLHDGSAPTLAAAVSAHS
                     GVTLSVADMDALVSYLNQIDDQPAGAPVPVTVVLESAAPAPVSGPFTVTATFSHAVTG
                     FTLSDITVTGGSASALTGGGANWSFTITPGADVSVSLAANIAQDAAGLGNAASNVLAR
                     IYGAPAPVLISEDIGNARVAGVTAHDTATGTYTLTADGEDIFFNADGFHFAKVLLTGD
                     GEIRARVRSLDNTHPWAKAGVMIRESAAAGSRHASAFITPPAAGNGFGMVWRPAANGA
                     TTYAGGPALNAVPDNWVRLVRAGNVITGYASANGSAWTQIQSVTLTSLAAQVQVGLVL
                     TSSDISQRANAVFDSVEILGSQAVSAPEAVLSAASAIETGAFTVQAQFSQPVTGLELA
                     DFTVTNGAASNLTGSGAAYAVTITPQSAGLVTVALPAGAVVNAASVPGTASNTVMVNY
                     APPVAATLQGQDVGDVGAAGSTSYDGSLYTLRGSGEDIFFNADGFHFALTQLSGDGEI
                     RARVTSQTNQNPWGKAGVMFRETLAAGSRHVMMFTTPTGAGNGFGMVTRAATNGATAY
                     TGGPALNAAPNNWVRLVRSGATLTAYASANGTAWTVVGTATLSGLPANVYVGLAQSSG
                     VRAQLGTATFDNVQVVGAQSVVAPAVTLSSASSVETGPFVAQAQFSQSVSGLTLADFL
                     VSNGSASNLTGSGALYSLTITPATEGAVTVQLPANAAQNSAGAGSTASNVLSVSYVPP
                     AVISLAGQDVGAVAVAGSTIFDGSTGQYTMTGAGEDIFFNADGFQFASTTLTGDGEIR
                     ARVTSQTNQNPWAKAGVMFREQLTGASRHVMMFTTPMGAGNGFGMVWRAASGAAANYG
                     AGPALNAAPNNWVRLVRAGDSFTTYASANGTAWTLVGNVTLTGMPDALHVGLALTSGS
                     TYQLSTATFDNVQIVSTGASGSSGSSGGSGSTPGSSNNKDTDFDGDDVNDLIEYAIGS
                     NTRYDAGLTLVSDAAGRVDAVLDVLGEAAGVAFTLEASPDLTGWVPLPLEPVARDVGS
                     GRRQLVWTGISHLNGQSPARGIVRLRATHTSGATAASTPQAWVRHDFGAGTQSAGVSL
                     VRAPVYAGFISSLGAEGTLLLDGALGAAVDAREEYYLEVRDGALAGHRLELSLLEEGR
                     AVADTAHTRGTLDQRQSASGLSAIT"
BASE COUNT         7440 a        14302 c        13374 g         7360 t
ORIGIN      
        1 agcacgccga gcttttgccc gcgccgcacg cgctcgccgg gcgccaccgc cacgctgctg
       61 agatgcgcgt agcgtgtgaa gacggggccg cagtcccagt catggcgcag cagcacttga
      121 ttgccatagc cgctgccgcc gcgctcttcg cggaaaacga cggtgccgtc cgccatggct
      181 cgcacctcgt cgagcggggt tccgtccgcg gcgcgctcca ccggggcgat gtccgtgccc
      241 tcgtggaatt gggtgaaaat ttccccgccc tcgtggagaa ggggggagcg cgtgaatcca
      301 aaggagccgc cctgccagac gcggctcccg ccccggtcca cacccatgaa gaatgcctcc
      361 ggcttgtccc cgagcagcgc gcggttcgcg gtggggaagt ccagcctcat gaccggcggc
      421 tcctggcacg gggctgaatg cccggccagg agcaggcagc aggcaaggcg ggcgcgcatc
      481 ctcatggccg gaaccaaact ccgccgcccg gcggcggcaa gcatttcccg cgcggcgtca
      541 cgactccggc agccagtccg acatctcgta gagtttgggg tcccgcgcgg ggccgcaggc
      601 gtggcggatc atttttttca tctcggcaaa gagcgcctcc ccttccccgc ccatgccgcc
      661 gaactcgccc tcgagcttct ccgggtcctc cccggcctca agcctgcgcg ccatttcgag
      721 cagcatgggc ggggttttgc cgcccatgaa atcggtcatc cggcgcatga ggcgtccgag
      781 ctggcgcggg tcggggtttt cctcgtcgag ggcgcccatg tccccctcca gcccggacat
      841 catggcctcc atcttttcgt catcgagtcc ggcgaagggg tcctggccgt cgtcctcctt
      901 cgccctgccg atgatggcga agcggctgac ctgcttttgc agcttgaagg cggggttgtc
      961 cgggcagcgc ggcaccacgc cgcgctgggc ggcggtgcgt gcgaggaact ggtagagctt
     1021 gtgattgtcc gggcagtaga actcgtagat gggcatggtg tgtgcgtggc tgaatcaggc
     1081 cgggaaaagc gagctggcaa gtcaggcctc gccgccggca ttggcggcgg cctcccattg
     1141 cctgcggtaa tgatcgagcc gctccttgat gcggccctcc aggccgttgg tggtcggctc
     1201 ataatagacg cgcccgccct ccaggcagcg ctggggcacg aagccgccct cgaagtcgtg
     1261 cgggtagagg tagccctcgc cgtggccaaa ctgcttcgcg cccttgtagt gcgagtcgcg
     1321 cagatgcttc ggcacgggca gggtgcgggc gtttttcaca tcctcgagcg cggcattgat
     1381 ggccgcgtag gcgcggttgc ttttcggcgc ggtggcgatg tagatgacgg cgtggctgag
     1441 gatgatgcgc gcctcaggca tgccgatgac ctccaccgcc tgctgggcgg ccacggcgac
     1501 gcgcagggcg ttgctgtcag ccaggcccac gtcctcgctg gcggcgatga cgatgcggcg
     1561 cgcgatgaag cggatgtcct ccccggcgtg gagcattttg gccagccagt aaaccgccgc
     1621 gtccgggtcg gaggcgcgca tggatttgat gaaggcgctg gcggtgtcgt agtgcgcgtc
     1681 gccgtcgccg tcatagacca cggtcttctg ctgcacggac tcctccgccg cggccaggtc
     1741 aatgagcacg cgcccgtccg cgccgggcgg ggtggagagc acggcaagtt cgagcgcgtt
     1801 gaggcacttg cgcgcgtccc cgtccgccac ggtggccagg tgccgcgccg cctccggcgt
     1861 caacaccgca ccgcgcccgc ccaggccgcg ctccgcgtcc gccagcgcgc gcgccatgag
     1921 cgactccaga gcctccacgt ccagcggctc cagggtgaag acctgcgagc ggctgaccag
     1981 cgggctgttg acataaaaga aggggttgtg cgtcgtggcg ccgatgaagc gcagggtgcc
     2041 gcgctccaga tgcgggagga gcacatcctg ctgcgccttg ttgaagcggt ggatctcgtc
     2101 cacgaagagg acggtgccct ggccgttgag cttccgcagc ctggcggcgg cctcggcctc
     2161 cttgcggatg tccgccacgc tgctctccac gccgctcaaa gtcacgaagc gggcctttgt
     2221 ctccaggctg atgatgtgcg ccagcgtcgt cttgcccacg cccggcggcc cgtagaggat
     2281 gagggaggag aagcggtccg cctccaccgc cctgcgcagc agcttcccct cgcccaggat
     2341 gtgcccctgc cccgcatact ccgccaggga gcgcggccgc atgcgcgagg ccagcggcgc
     2401 gtccggcgcc gcctccacct gcgcgggcgc ggtgtctgcg ggggaaaaca agtcgtcact
     2461 cacacccacc tcataaccca ggaaccgggg gcaatgcaaa cgcatggagc gcggccccgc
     2521 ctgatccggc gggcgggagg cttttaggca aaggcacgct ggcgctccac cgctgacggg
     2581 cgtattttac acgtgtcaac cgcaagcgaa ctctcccatg aaacacccgc tccccatcct
     2641 tgcctgtctc gtcaccagcg cgctgcatgc cggtgatccg ccgctttctc ccaccgtggc
     2701 gcccgtgccg gacacgccgt ggtatgagcg tcacgcgctg gattttgaaa ccggctggct
     2761 gggaaaggtg ggcggcagca cgccgctcga ttacagctcg gtcccggtca tgctctcctg
     2821 gcgctcgccg gaggtgctgg ggctgcattt tgacggcggc tccgccctga tcttccgcaa
     2881 ccggctcacc ctgctcggcc agtggttcga gaccggcgcg gagaatcatt acttcggcgt
     2941 catgggcgcg ccgtcgtttg aatactggtc cgcaggaggg cagtggtcgc tttacctcca
     3001 ggtgggcggc ggcgcgggct ggctggactc ccagggcgtg cgcggcggcc aggggcagga
     3061 tttcacgctg aactggttcg ccgcctccgg catgagctac accctgcgcg agagcgtgca
     3121 ggtgcgcggc gggctgatgt tccagcacct ctccaacggc ggcgccaccg acccgaaccc
     3181 cggcctggac agcctgggct tcaccatcgg cctgtcgtgg ggattctgag gagagcggac
     3241 aacggggact ggatgggtgt gtggcaaaaa attcaccttt gcctggattt gggatttaca
     3301 ttgcgcgcgc gggctggcta ttctccgcgc aattatgagc gagaaattct tccccatgcc
     3361 ctccagcctc gaacctcttg agcaaaaaat cgcccctgcg ggcaccgtca tcctctccac
     3421 tgcgggcggc gtgctcacca tcacgggcga cgcctccgac aacggcatcg gcatcaccca
     3481 tgtgccctcc accggcatgt ggaccatcac tgacccgctt gccggcacca gctacatcct
     3541 caacaacggc gccccccagg ccgggggctt caacatcccg gcgcagagcg ccatcgtggc
     3601 caacctgggg gacaacaacg acaggctcga catctcgccc tccggcacgc ccagcggcct
     3661 tgtgctgaaa gcgctgacca tcaacatggg caacggcaac gatgtcatcg tgatgggcac
     3721 ggtctcggcg cagaatctgc aggtcaccgg agccaccacc atcaacctgg gcgaggggaa
     3781 cgacaccctg aacaccacgc aaagcgccac ttatggcggc ctggtgaaaa tcctcggcgg
     3841 cggcggcaac gacacggtca atatctcggg cgccagcgga gagcaggtgt tcctcaaggg
     3901 gctgaacgtg gacctgggaa ccggcaatga caatttcaac gcgaatgtcg cgcgcttctc
     3961 cgtcgccggc ggctccctgg tcgtgaagaa caccggcacc gccggaggag cgtcctcctt
     4021 caacatcaac tccggtctcg cgatcatcac cgtgccaacc gtcttcagca cgagtctggc
     4081 cgacctgagc gtcaacctgg gcaacaacat ggcggacgta ctgcacttcg gctccaccgt
     4141 ctccgtcatc ggcggcaacg gcacggacgc cgtgaatgtg aactcgcaga tgaccgccac
     4201 cagcaccgtc accttcgacc tcaaaaacgg tgcgaacact accacactgg tgacggacgg
     4261 gagcttgact ggcacttccc tcgtcgtcaa aggcggcacg ggagatgatg accttgctct
     4321 tcaggactca cacgatctgc tcgtcaccgg acagctcaat ttctccgccg gcaacggcac
     4381 cagtactttc attgcggatg tcaacagcac gctgctggcc ggctcgctcg tgctcaatgg
     4441 cggcacgggg atcgacattt tcagcttcgg aggcacatcc ctcaatgtga tgggatcctc
     4501 cacattcaac atgggcgcag gcgcgaacaa caatgtccag cttgccggca ccgcgtcctc
     4561 cttcattggc ggcagccttt tggtgaacgg ctccgacggc accgatcaga tcgtgctcga
     4621 cagtccgcaa ttcaccatcc tcggcagcat caacaccaag ttcggcaacg gaaccaatgt
     4681 cctgctcgct gaaggcggca gtgtttacat cggcggcggt gtcaacttca gcggaggctc
     4741 tggatcggac gtcctgcagg cgcagagcac ctccctgatc atcaacaaat ccaccgtctt
     4801 caacaccggg gcgggcggca acaccctcta ttatcgccca gactccggca ccgtcggccc
     4861 tgtcacctac aatggcggct ccggaacaga caccttcgcg ctgggaaatg ttgacggaac
     4921 cagcaccacg cgcctttcgg tcaatggcgc ggtcacgacc aacttcggcg cgggaacttt
     4981 caccagctac tacacagaca ccatcgtgca tggcattgtg aaccacaagg ccggcgcgct
     5041 cgccggggag aatgaaaaca tcatcatcag tgaatccacg ttcaattccg cggtcaacat
     5101 cctgctcgga gctggaaacg ccgatgtcga catcaacgac gtcttcgtgc gcggcgcctt
     5161 cacactcgac acgggcgcgg gcaatgacca ggtcaacgtt gacacactgg gaggcagcag
     5221 cgcattcagc agctggttcg gcatggtgaa aatcctcacc ggcgcggggg acgacatcgt
     5281 ggtcatcggc tcgagccccg tcgtggtggc aaacgccggc aacaacttct tcagcgggct
     5341 gctggtggac ggcggcgcgg gcggaggcga cagcttcacc cagggcaaca acgtctttgt
     5401 cggaaccaac aaccaggtca acttcccctg acctggcggc gcgtgaggct ttttggaaaa
     5461 ccggccgctg gctcacgcgc caccggccgg tttttctttg gctcaatacc agcgcgccag
     5521 accttccttc cagagcggtt tgagatcggc gatcgagctg tcaatgagcg tggtgccgcg
     5581 ctgggtgatg tgcagccagc gctcgccggt gctctcgccg atgatgactc cgccggccag
     5641 tttcaccccc tcgacatgct cgggtgcgac ctcgatgagg aggcggcccg gtgtctcgcc
     5701 aaagagcact tcggcggtgg tgcaggcctt gtcggcgggc acgttttcca gctcgatctt
     5761 gatgcccgcc ttgccgctga aggccatctc ggccagggcc acagcgagac cgccttccga
     5821 cagatcgtgc gcggcgagga tggcaccctt tttcacgagt tcataataac gcccgtaaga
     5881 cgcccaagcc tcggcgtcat cccaggcggg cgcgccattg agaccggcac cctgggcgtg
     5941 cttggcgaag acgctgccgc gcagacccac cgtggttttg ccgagcaggc agagcttgct
     6001 gcccgcgcgg cgcagtgacg cgccaacgac atgcgaggcg tcctcaatga cgccgaagcc
     6061 gctgaccaga agtgtgaccg ggatggagac agggccttcg tcagtgatga agtaattgta
     6121 aaagctgtcc ttgccggaga cgaaaggcgc gccatacgcc agcgcggcct gggccatgcc
     6181 tttcgtgcat tccaccagcg cgccgagctc gcgctcgttg tcggggttgc ccatgcagaa
     6241 gttgtcgagg atggcgatgc gccgcgggtt cgcgcccatg gccacgagct ggcgcacgca
     6301 ctcatccacg caggcgcgcc ccatggcgtg cgggtcggcc ttgccccact cgggcagcaa
     6361 ggcgcaggcc agggccacga gctggcggct gccatccacg gcgattacgg agccgtcttg
     6421 cggcgcatca ccactggctc cggcgagggg ttttaaaatc gtgttgccct gcacctcgtg
     6481 gtcatactcg cggatgatgg gctcgcgcga gacgatggcg aagtcggcca gcaatgtctt
     6541 cagcacctgg gtccaggact cggggcggac gctgatcttc ggtgtcttgg cctccggctg
     6601 ccgccaatgc gcgcgcatct cgcggcgggg cgcgccgtgc agtttttcca cgggcagatc
     6661 acacaccgca tggccgtgat gccagacttt caggcgcttg ccgccatcgg ctttggccag
     6721 cacaaacatc tcgctctgcc aggtgtcggc cagcttttgc agggcgggca ggttttgctc
     6781 ctccacggcc atgaccatgc gctcctggga ctcggaaatg aacacctgcc agctctccag
     6841 gccgggctcc ttgagcgggc agttctccag ccacacctcc ccgcccacct cgctgagcat
     6901 ctcgcccgcc gcgctggaaa agccgccagc gccgcagtcg gtgacgaact caatgagacc
     6961 cgcctcgcgc gcggcgagga cgaagtcggc cacctttttc tcctcgatcg ggttgccgat
     7021 ctgcacggcg gtctggtcct cctcgtgcga gtcggtggtc agctccgccg aactgaaagt
     7081 ggcgcctttc agaccgtcct tgcccgtgcg cccgccggcg gcgatgagca ggtgaccggg
     7141 cttcacttcc ttggcgatgt ccttgatggg aatgacgccc gccgtgccgc agaagacgag
     7201 cgggttgtag atgaagctgt cgtcaaactg aatggccccg ttcaccgtgg ggatgcccat
     7261 gcggttgcca tagtcgcgca caccgcgcac cacgccgcgc atgacgccga gcgggtggat
     7321 cacgtccttc gccttcaagt cttcctgctt ggtgtccggc gggccgaagc agaagacatc
     7381 caggctggcc accggcttcg cgcccttgcc cgcgccgagg atgtcgcgga tcacaccgcc
     7441 gaggccggtg ttcgcgcccg cgtagggctc gatggcgctg gggtggttgt gcgtctcgac
     7501 tttcaggcac acggccttgt cgtcatccag cttcacgaat cccgcgttgt ccacaaaggc
     7561 gctgagcacg aagtccggct tctgctccat gatgcgctcc gtggtggcgc gaatgtaggt
     7621 tttgaaaagg ctgtccacca cctccggcac gccattcagc gtgtgctcga tgcgggcgtt
     7681 gaaaatgcgg tgcttgcaat gctcgctcca ggtctgggcg atgacctcca actccacgtc
     7741 cgtcggttcg cggccttcct ccacatagag cttctggacc gcctccatgt ccgcgcgcga
     7801 gagggagagt ttcatctgct tgctcagtgc caaaagcgcg tcgccgtcga gatcacggac
     7861 ggggatggtg cggtagatgg cgcgctcggt gcggggggcg gaggaaaggt cggcttgcaa
     7921 ggaatggagg gggcttctca tcgtcttggc aagaaaagcg gaccggtcaa cggcggcgtt
     7981 cgcggtggcg caaacaatgc ggtcaggcgc tccgcacatt cactgccggg cgggtttatc
     8041 cgccaaaccc tccttccaga gaaatgccgc tcatcccgga gccgtaattt gtcctccgcc
     8101 atgaaccgcc tcctgctccc gctctgcctg ctcgccgccc ctctccaggc cgactggccc
     8161 acctatctcc acgatgccag ccgcgtggga cactcgccgg aaaaccttgc cacgcctctg
     8221 cagacccgct ggacttatag ctcatccgtg gcccctgatc tggcctggcc cggcgaggat
     8281 ggccgcgaga tcgagggcat ggtgctggaa aaccgcatcc gctttgacga tgtcttccac
     8341 accgccatcg ccggcgggcg cgtgtatttt ggctcctcgg tggacgggcg gctctactgc
     8401 caggatctcg ccacgggcaa agagctgtgg gctttcttca cggacggccc catccgcctc
     8461 gcgcccatga tcgccaatgg caaaatctac gtcggctctg acgacggcca cgcctactgc
     8521 ctggatgccg ccaccggcgc cctcgtctgg cgtctgcgcg ccggaccgga cgacgagcgc
     8581 gtgcttgccc gcggccgcat gatctcgcgc tggcccatcc gcaccggcgt gctggtggat
     8641 gacggcgtgg ccttcttcgg cgcgggcgtt ttcccgcatg aaaaggtctt cgtccatgcc
     8701 gtcgaggccg ccaccggcaa ggtgatctgg aagaacgaca ccatcagcca gcaggacgcg
     8761 gggcgcagcg acctctcgcc gcagggctac ctgctcgcca ccaaggacct gctctttgtc
     8821 ccctccggcc gcgccctggc catggccttc gaccgccaca ctggcaagcc cgtcaacaaa
     8881 ccctcccccg gctggcgcgg cgacgccggc ggccagatcg gcggctccca ggccatgctg
     8941 gtggacgacc aaatcctcgc cgtcggcgag catcaggtcc tggcgctcga tcaaaagacc
     9001 ggcaaaaccg gctacgcctg gttccgcggc acgcagatga ccttttccgg cagcaccggc
     9061 ttcatggcca acggcaagga gatcaccgcc attgaccgcg atgcccacgc ccgcagcacc
     9121 caggagcggc acgacctgga gatgaaagcc tacaagctca acggcgacct gcgcaagcac
     9181 cccgccctgg ccgagctcaa ggccgtgacc gccgccgaaa aagtcctcaa agaggccaaa
     9241 gccagcggcg gcgatgccgc caaagccgag gcagccctcg ccaaggccgc ccagaaatac
     9301 gagccgctgc gggtggatta cgaaaccaaa aaagcccagc tcgccggcta ccggaagcgc
     9361 ctggaggaat tcaaagaagc cggcatcaag tggagcaaac ccattccgca cgagtccgct
     9421 ctcatcctcg cgggcgacac cctcgtcatc ggcggtcgaa acgaagtgct ctgcctcaat
     9481 gccgccaccg gtgagcaaac ctggaaaacc gccgtcgaag gcgaagcccg cggcctcgcc
     9541 gccgccgacg gccacctcct cgtcagcacc accaccggca aagtctatgc cttcgccgac
     9601 gccgccctca gcctgccgca gctcgcgcaa acaccctccc tcgaaatgaa tcccttcccc
     9661 caggacgacc tcactaccct ctacgccgat gccgccgagc aggcgctccg tcagagcggc
     9721 gtcaccgacg gctactgcct cgtcatcggc agcgagcagg gccgccttgc ctgggagatc
     9781 gcccagcgca gcacgctcaa ggtttatggc ctcgagcccg atgccgaaaa agtccgcgcc
     9841 gcccgagaga ccctcgcccg caccggcctc tacggctcgc gcatcgtcat tgaccacctc
     9901 gacctcagcg tcatcccgca cgccagttac ttcgccaacc tcatcgtctc cgaccacctc
     9961 ctgctcaccg gcaaaatgcc cggcctcccg caggaagtcg cccgcgtctt gaaacccatc
    10021 ggcggcgtca tggccctcgg cctgcccgcc agcgctcctg ccgaccgcca ggccgcagcc
    10081 aaagccagcg tcgccgagtg gatcaaagcc accggcctgc ttgaggagaa agccaggctc
    10141 gaaagcaaag acagcctcac ccttctcacc cgcgccgcat tgcctggcgc cgactcctgg
    10201 tcccaccaat acggcaacgc cgccaacacc tccagcaaca aggacgagcg catcaagggc
    10261 ggcctcagcg tgctctggta tggcgacccc ggccccggca agatggtcaa ccgccacgtc
    10321 ggcgccgtcg gccccatctc caccaacgga cgcctcttca tccagggcga atggaccgtc
    10381 atggcctacg acgccttcaa cggccgcttc ctttgggaag tcgaaaaccc cggcgccctc
    10441 cgccacggtc tcaaaggcgc ctacgaaccc ggcaacatgg ccgccagcga cgaccacctc
    10501 ttcgtcgcca tcgaggacca ctgcctccag ttcgacgccg ccaccggcag gatcgtccac
    10561 cagttcaagc tccccgccca ggacctccag cccgacaaac aatggggcta cctcgcctac
    10621 gaagacggca tcctctacgg tgccgtgacc gaccgcaagg agctcgccgc cgtccgcgcc
    10681 cgccgcacca aagccgccgc ccaggccagc gacgccatct tcgcctacga ggtcgccagc
    10741 cagaagcttc tctggcgtca ccagggccag tacatctccc acgtcaccat cgccatcggc
    10801 gacggccgcg tcttcttcgt ggacgcctcc ctcacccccg atcagcgcga cgacctcctc
    10861 aacgccgaca aaaccgagct cgccaagctc accggcaaag cgcgcgaact cgccgaggag
    10921 cgcgccaaag ccgccgacgt ccgcatggcc accgccctcg acgccaggac cggcgtcaaa
    10981 gcctgggccc agcccgtgga tgtcaccgac tgcagcgaga tcggcgccgg cggcggcagc
    11041 ctcaccctca tctaccacaa cggccacctc gtcctcggcg gcgccaacgc caacggccac
    11101 tactgggagc agtacctcgc cggcgaattc gagcgccgcc gcctcgtcgt gcttgacgcc
    11161 gaaaaaggcg gcaagctttg ggccaaggac gccaactaca aaagccgccc caccgtcatc
    11221 aacaacgaga tcatcgccga gccctggggt tacgacctct ataccggcgc gccgaagacc
    11281 cgccagcacc ccctcaccgg cgaggacacc ccctggctct tctcccgccc cggccaccac
    11341 tgcggcgcca tctccgccac cgcccacctc atgttttacc gctccaagac caccgccttt
    11401 tacaacctcg acgccgacga aggcaccgag cactttggcg gccagcgcct cggctgctgg
    11461 atcaacacca tccccgccaa cggcctcgtc atggtccccg aagccagcgc cggctgcgtc
    11521 tgcctcttct ccatcgcctc caccctcgtc ttcgagccgc gcgagcagag ccgcatgaaa
    11581 tggggcgtct atggcgccgc gggcgacacc cgccccgtcc gccacatggc cctcaacctc
    11641 ggcgcccctg gcgaccggcg cgacgcccac ggcaaactct ggctcggcta cccgcgcccc
    11701 tcctcccgca ccggcattga cctgccgttg aagctcaccc acacctttgc caaagacggc
    11761 gccttctacg acgtgaacga ggaaaccctc gccatcgccg gcaccgacac cccctggctc
    11821 tacgcctccg gcgccaaagg cctcagcaag ctggaaatcc ccctgctcaa ccccggcgac
    11881 gcctcccaga cctacgaagt ccgcctccac ttcaccgccc aggaagacca gcgccccttc
    11941 accatcaagc ttcagggcaa acccgtcgcc accgccctcg acctcgccgc cacgcccgcc
    12001 gcccgcatcg aaaccttcac cggcatcccc gtcaccgaaa ccctcaccgt ggagatcgaa
    12061 cccacaacaa aagaaggcac cctccccacc ctccacgccc tcgaaatcct ccgcaccggt
    12121 gagaaggaaa tcaaagccgg cgtcgccgcc cgctaacccg ccgccgaaag atttgctgca
    12181 ggcgctcatc ccgtgaagtt ccattgagaa atggaccgat tccaaatcct gaatcccgct
    12241 gcctcgctcc gatgggcggt ggtcgccgct ttgttgtcat ttccagcgga ggcatggtct
    12301 gacgactggc cgcaattccg cggtctcaaa cgcgacaacg tctggcgtga aaccggcctg
    12361 ctcaattcca tacccccgga gggcctgaaa attcgctggc gacagccgtc tggtccgggc
    12421 tggtcctcac cggccgtggc cgaaggccgg gtgttcctca cggatgcgga actggccaaa
    12481 ccagacgcaa gggaacgcgt tcgctgtttt gacgccgtga ccggcgaagt cctttggtct
    12541 tatgcgtacg acgtcgctta tcctgaatgg gctttcgttc ccgggcaggg cgggggtccc
    12601 acgccgacac cggttgtcga agctggccgt gttttcacgg tgggagccaa cagtcgagtt
    12661 cactgtcttg acgcggccac cggcgccgtc ttctgggact atgatccgcg gcaaaaatac
    12721 caggtgcgtg acatgcagtg ccgcggctca cccttgattg atggagaccg gctcatcgcc
    12781 ttcatcgaga ccaagccggg ggcttgtgtg atcgcgctcg acaagaaatc aggcaaagaa
    12841 atctggcggg cagttgatga gcgcgtgtcc aacagctcgc cgctgatcgt ggaggctggc
    12901 ggagagcggc agctcatcgt ctggacggac gactccgtca cctcgctgga tcccgtcaca
    12961 ggcgagccgt ggtggaggga accgatgacc accagcaaca atgattcggt gccgactcct
    13021 gtttttgaca agaaccgcct gctcatcagc ggtgtgatgt ttgaactcag cacttccgag
    13081 ccaacggcca ccatgctctg gcccggaaag gacgttgccg ctcccaagcg gctgctcacc
    13141 aataccgggt cgccctgctt gcggggcgat cacatctact ccgccaaatt gaacggcgaa
    13201 ctcgtttgtc tcgctgccaa gacaggagag atcatctggc gtgacaccac catcaccagc
    13261 cgcaagggcg gcgccgccat ccaccttata cccattgatg atgcgagcgc cttcttcctc
    13321 ttcaccgatc aaggcaacct catccgcgcc caacttactc cgaagggtta tcaagagctt
    13381 ggccgcgctc ttttgatcaa gcccaccact ccgttcgcgg gcagggattg cgcctgggct
    13441 ccgccggcct ttgccaaccg ccacgtcttt gtccgcaatg acaatgaaat cctctgcgcc
    13501 tcactggcgg cggaggaaac cgccccccgg caatagacac agaccacggc cagcccgtcg
    13561 ccggattcat gccccgtctt gaacgccctc gaaatcctgc gcaccggcga aaaaaaaaat
    13621 caaagccggc gtggcggcgc gctgatccgc tggtgtccag gcttcagccg atctgctgga
    13681 cgaccaccca ttggcggcct gccgcctccc ttatccgtaa cgatgaagtt gaaccacgaa
    13741 tggacacgaa tccatgcggg gaagataggg atgggggcat cacccagggg tgatctcccg
    13801 gcgcttcagc tcacctgaca tgatgcctcc aaaattcgtg ctcatcggtg tccattcgtg
    13861 gttttccatc gcatgaacac ggcttatcgc ccagagcgtg tcatgcacat caggaggttg
    13921 ttgagctgag gaagtcggtg gcgggccatg cagaccggcg agcgcgtccg gcagcgcgcc
    13981 gggcagcggc ggcggggtga acacgggcgg gtcaaacgcg cccccgccct ccccccggta
    14041 gagcgtctcc accgccgccg tggccgtctc gccattgccg cgcgcccagg tcagccaggg
    14101 gccgcagagg tattcgcaat agagcgcgtc agccacgcgc gcgttgaggg tcgtcgtgtc
    14161 cacgcccagc acgggattca ccaccagcag ttgcgtggcc aggttgtgga accacgccgg
    14221 gcgctcggag acgcgataag ggaagtaatg tttacgtctc atgtttttac ggtgtttatg
    14281 gtttctgtcg cggtttctaa acgggtccgg ccccgtggcc ggaccccaaa gggcgccgga
    14341 ggaccaagtc actccgctgg accaggtcgg catggcaaaa gattgatttt caacgggtta
    14401 atcaagcgca cccggcgcgt cttcaggccg cgcggaggct tgccggaatg aatccagcat
    14461 gtgctggatt cattccgaca cgtgccgggc tcactccagc atgtgccgac gccactccgg
    14521 catgtgccga cttcactccg gcatgtgccg acttcactcc ggcatgtgcc gacttcactc
    14581 cggcatgtgc cgacgccact ccggcatgtg ccgacgccgc tccggcatgt gccgacgcag
    14641 ctccggcatg tgccgacgcc actccggcat gtgccgactt cactccggca tgtgccgact
    14701 tcactccggc atgtgccgac gccactccgg catgtgccga cgccgctccg gcatgtgccg
    14761 acttcactcc ggcatgtgcc ggagtcattc cgacatgtgc tggattcatt ccggcatgtg
    14821 ctggattcat tccggcatgt gctggattca ttccggcacg tgctggattc attccggcat
    14881 gtgctggatt cattccggca cgtgctggat tcattccggc acgtgccgga gtcattccgt
    14941 gcgccgctca cagagggcac cgtcatctgg taaagcccgc ccgccatccc cgcttgtgcc
    15001 cgcccggcaa ccgcccacaa tggcgcgcca tgcctcccca agaaaccccc ttgaccgcgc
    15061 ttagcgcctc tattggccgc tggccgctga gccaaaagcc cggaccggcg gctgactgcc
    15121 gccaaaacgc ccggggatgc ggatgtccgg catcttccgc cagttccaga ttcaacacct
    15181 cgcgatacaa atacagcacg gcattgagcg cctggaaagg ccttggtcag accccgcgcc
    15241 cgttcccccg tgggaatggc ggaagcccaa gccgaaaacc agcgcggtcc tcaatggcga
    15301 ctgagttctg agttgagttt ctagctggat gctcaactga tgactgcggc ccaaggtctc
    15361 tttggagttg gtcaatcgcg gcgagcatcg gttagttggc cgaacgatca agcgctggca
    15421 ccgctggggc gggggtgaga gtcgatacgg gatggcgggt tagagtcacg gaaagcgcgg
    15481 ccgacagtgc agccccagcg gttgtccagc cgcgccttgt tcgcctttat tggttggtgt
    15541 aggcagtgta tcatggctgc ttcaaatgcc acgagaactc tggaagagca agcttagtag
    15601 caatggcttt gtaggcgtct ggtgtgaatg gaccggaaac atctccctca ccagagacct
    15661 tcttcgcaat gtaatagtgc tcgataccac ccgatgtcag ttgcctcgtg aaaacgacgt
    15721 aacgcgaatc agatcccgca gaaatcactg tcgggtcact catccccaag atagccccgt
    15781 cacccatata gtgccccatg atgatctctt ctgaagcagg acgagtataa acccgatagt
    15841 tgccgtcttc ccagtgcttg atcacgcctc gtccacatcc gctcagtgcg aggacgagga
    15901 atgctaaagc tggaagtcgc atgacgggtg gcgtgtggga gttggccgaa cattgtggat
    15961 cattcacaaa aatgcagctt tgccagcagc tttatctctg acggatttgt tgctttatga
    16021 cggcgttttc gattcatgac gctgctctta tgaatcattc gcgcagcaat caacatccat
    16081 gctgctttcc gcgcaaatac ggggctgaaa tccatgtgtg actcctccac gcacgaagcc
    16141 agccctgggc agcggctcat cggcgtgacg cggttcccgc cgcgaacgcc ggggacgccg
    16201 gcctgcgttt ctgaagggct catccgcagc ctgggcaggc ggatcccggc aggctggggc
    16261 atgcgcgcct tttcactggc tttcccggcg cggctgtggt tgatgcgcgg catgaacgag
    16321 ctggaggcga atccctggac gactctcgaa acgcgcaccg cctgccgcgc cttgagcacg
    16381 ccgacggaac gatggacgcc ggcgagcacg agcgccgggt aggagagatg gctcattttc
    16441 atcatcgcga ggctaccgcc ggtccccaac gcgccacaag aaaaaaacag cccgcctccg
    16501 ccactcgacg caccttgcgc ttcagccagt ccatgcactc ggcgggcgat catcggatac
    16561 ttgcggggca gaaggcgcgt cgtcaaagca ccacgttctt ccatgcgacc ctgtggcgag
    16621 ggctggattt gtcccgcctg gtttgccgtc tggcgctcga cagccattgt catcgaaggc
    16681 gacggccttt cttgcgcttc cgtcacgcgc gtggaaggtg gcgcatgttc accggactcg
    16741 ttgaaaccac aggccaggtc atcgccctcg aaacacgcgg agaaagcgcg cgtctgacgc
    16801 tggacgcggg gccgctcgcg gcggagctgg ccttggggga gagtctggcg gtcaatggct
    16861 gctgcctcac cgtggcggaa ctggacgcgc gatcagggcg cgcgggattc gacctgctca
    16921 tgcagacgct gaaggtgacc agcctggggg atttgcagcc gggctcgctg gtgaatctgg
    16981 agcgggcgat ggccctgggc gcgcgcttcg gcggccactt cgtgcagggg catgtggatg
    17041 cgacggcgcg catcctggac tggagccggc acgggcagga ccaccgcctg gaggtggaac
    17101 tgccgcccgg gcaccgcggg ctggtcattc ccaaagggtc catctgcctc gacggcatct
    17161 ccctgaccgc cgccgaggtc ggcggggaca gtgtcacctg ctggatcatc ccgcacaccc
    17221 gcgcggtgac gcatctgcgc ggcaaaaaag cgggggaccg ggtgaatgtg gagttcgaca
    17281 tgctgggcaa atatgtgcgc gaactgatgc gcgcgggctc gcctgccgct ggagctgcat
    17341 cttgacggca tcccgccttt ccggcatcat gcgcggcggc cggaggaggc cggtccgaaa
    17401 tcccatgctg aaagtttttc attcccaccg gcccgtgttg cgcctggcct gctgtctttc
    17461 cctcacggcc gccggcctgg gcgcgcagga acctcaggag acgcccaagg ccctgcctgt
    17521 ggagtcgctc ggcacgatgc cggaagcgga tgcggtgaaa aaaaagcgcg acctctcagc
    17581 cagcctgctc acacagaagg aggcgcgcac catgcgcctg agcatcccgg cgccgcgcgg
    17641 ccagatcgtg gaccgcaacg gcatcgccct ggccaccaac aaggtggtca actatctggc
    17701 cctgaatttc cccttcatgg aaaaggccac cccggagaaa atcctggact acgccaaaga
    17761 gcgcatcaac gcggtgaacc aactgctggg caaaaactgg tccctgccgg acgagcgcct
    17821 gctcacccac tacgagcacc gccgctggct gccgctggtc ttttccatcg aggaggggct
    17881 gaatgtggag atcacgtccg agcagcaggc caagctcaaa ctgatgcttg aggagggatc
    17941 cctcctgctc cagcccgcct acctgcgcca ctacccgcac gaggacgccg cctgccacat
    18001 cgtcggctac accggcaaga cacgcccgct gccgcaggga ccgatcgtgg acggcgaccc
    18061 gttgtttgag gagatggagg ggcgcgaggg gctggaggtc accttcgatc acttcctcaa
    18121 aggcgtgccg ggcgagatca acctgctttt tgacccgaat ggcaagctgc tggccgaaga
    18181 ggtgctgcgc cgcccgatgc ccggccacac cttggtgacg acgctggact tcaacctgca
    18241 aaaatacgcc gagaacgcct tgaagaagac cgcgcgcagc ggcggctcca tggtcatcat
    18301 ggacgtgcgc aacggcgaca tcctcgccat ggcatcgaac cccggcttca acctcaacga
    18361 gttcatcccc ggcatccgct ccgcccgcta cgaggagctg gccaacgacc cgacgatgcc
    18421 gctttttgcc cgctccttcc gcggtgaata cccgcccgcg tccaccttca agatcgtcac
    18481 cgcgctcggc gcgctggaga gcggccgtgt cagcgccgcc accagctttc cctgcacggt
    18541 ctcgatgctc gtgggcgacc gctattttca caactggaac accaagaacg acgagggtcc
    18601 gatgaacgtc atcaccgccc tcaagcgctc ctgcaacacc tggttctatg ccgccgcgct
    18661 ccaggcgggg gccgatccgg tgacgaacat ggctttccgc ctcggcttcg gcgagcgcac
    18721 cggcatcccg ctcaaagccg agggccgtgg caacgtgccc agcagcgccg accaccgcat
    18781 cctcggcggc gagctggcaa acatcgccat cggacagggc gccgtgcttg tgactccgct
    18841 gcaggtttgc cagtccatgg ccgcgctggg ggacgggcag cgcgtctttc agccgcggct
    18901 ggtgaaacag gtgcagacca tcaacgagat cgtcgtggat gccacggagg aaaagaaagt
    18961 gcggcgggag ctcatgttaa accccgagtt ccgggaaacc atcctcaaag gcatggtggc
    19021 cgtcgtttca ggctcgggcg gcacgggccg ctccgccgcg atcccgcagg cccaggtggc
    19081 gggcaagacc ggcaccgcgc agtggcgcgt ggccaaggac cagcgcctcg cctggttcac
    19141 cggtttcctg cccgccagcc agccggtgct cgccttcgcc gtcgtctatg aagggcgtcc
    19201 cggtgaaaag atcggcggcg gcgcggtggc agcgcccatt gtgaaggagg tcttcaccaa
    19261 attctatgag ggcgcgccca cggatgatcc gctcgtggcc gccatgaagg acgtgcccct
    19321 ggctctggcc gtggacgagg gtgtcatgga cgatgtgcca ctggcccagc aagtgcgccc
    19381 cgccgagacg gcgccgcctc cgcagactga gcagcggacc ctgggcggct tcttcaggag
    19441 gctcttcaag cgcagcaatt aacccccttc cctttcgtga tgacagtgca gttggccacc
    19501 ctttgcgact cggcttccga ttactccggg aagctctgca tcctcggagc cttcgacacc
    19561 ctttgcgcgc gcgagttccc ggtcgtgcat ccgcattgcg cgctcgtcgt ccggctcatc
    19621 ttcgagccgc gcgacgcggg acgccacacc ttcaccatcc gctgtcttga cccctccggg
    19681 caggactgcc tgccgccctt cgagccagtg gtggatgtgg ctttcccatc gcagttcctg
    19741 ccctttgtga cgcgcaacat cgttctcaat ctccagcgcc tgcgcattga aaagcagggc
    19801 gtttatcgct ggaacattga gaccggcggc atcaccctcg ccagcatccc gctgcgcgcc
    19861 acgctcttcg aggacccgcg cggcgcggcg ggccccgccg gttgatccac cacactccca
    19921 tccccccgtc atggacagtc ccgtcttgca ggaaatgccc gtcaaagccc ccgccggcca
    19981 tgccatccgg cagttgtccg tgtttctcca aaaccgtgtc ggctccctgc tcgcgctcat
    20041 ccgcctgctg cgcgagcact ccatcgaggt gctcggactc tccatgcagg acaccactga
    20101 gatgaccctc gtgcgccttg tgctcagcga ccccgacggc gccggcatgc tgttcatcga
    20161 aaaaggcatc ccgcacaccg actgccccat catcgtcgtc gaactcggcg agtccggccc
    20221 cggcctggcg gagtgcctct ccgccctgct caccgccgaa ctcaacatcg aattttgcta
    20281 ccccctgctc gtccgccccg gcctgcatcc tctgctggcc ctgcactgtg atgactgcga
    20341 catcggggcc gaggtgctcc acaacgccgg cttccgcgtc cactcgcaag gcgatttgag
    20401 ccgctgaaac aaaaaagcgc gcaggcttgg acccgcgcgc cttcgcagga ttttcgagct
    20461 cctcagcagc cccacacttt gaaaagcgcc tccgccatgt cggcgggtga gtccgcgaca
    20521 gaaatgccgc actcggccag gatgcgcttc tttgccgcgg cggtgtcgtc cgcgccgccg
    20581 atgatggccc cagcgtggcc catgcggcgt cccggaggcg cggtggctcc ggcgatgaag
    20641 gcggcgatgg gttttttgca gttctctttg gcccagcggg cggcctcgac ctcggcgttg
    20701 ccgccgatct cgccgatcat gatgatggcc tccgtctccg ggtcgtcgtt gaacattttc
    20761 agcacgtcga ggtggttggt gccgttcacg gggtcgccgc cgatgccgac acaggtgctc
    20821 tggccgtagc cgcgagtggt gagctgccac accgcctcgt aggtgagggt gccggagcgg
    20881 ctgaccacgc ccacattgcc gcgcttgtgg atgtagccgg gggcgatgcc gatgcggcag
    20941 ccgccctggg atttctcacc acgtccgggc gtgaccacgc cggggcagtt cggaccgatg
    21001 aggcgcgtgc ggctgccctg catcgccgct ttcactttga tcatgtcgtt gacggggatg
    21061 ccctccgtga tggccaccac gaggtccagc ccggcatcca ccgcctcaag gatggcatca
    21121 gcggcaaagg gcggcggcac gaagatggcg ctgacggtgg ccccggtctc gcgcgcggcc
    21181 tggcccacgg tgtcgaagac gggcaccttg tggccgccgt ggtcaaacgt ctgcccgccc
    21241 ttgccgggag tgacgccggc cacgagctgc gtgccgtagt cgagcgaagc tttggcatgg
    21301 cgtgagccga agtcgccggt gatgccttgg acgaggattt tggtgtcagt gtcaacgagg
    21361 atggccatag gtgtggggaa aacgaacgct gggtctttag cgagggcggc agggtgcgaa
    21421 actcgaaatt cgaatttcga cgcatgaatt tcacgctccg cgcgctggcg cgggatttca
    21481 gggggcaaac tcgcgcattt tcagcctcaa ctggccgccg tccttcggcg ccaccacgcg
    21541 gcggtaaaag caggtgggcc gcttggtgtg gcagcagccg ccgccgtgct ggatgacgcg
    21601 cagcaccagc gcgtcctggt cgcagtcggt gaggatttcc acgatctcct ggaattcgcc
    21661 ggtggtggca cccttgtgcc agagctgctt gcggctccgg ctgtaataga ccgcctggcc
    21721 cttctcaaaa gtcatcttga gcgactcctc gttcatatag gccagcatca gcggctcgtc
    21781 ggtgcgcgcg tctatcgcca tggccgggat gaggccgtgc tcgtcgaact tcggggcgaa
    21841 accgcctccc tgctcgacgg actctttcga gtcacgggtt gcgaatgtgt attttttttc
    21901 ttctgacatg ggggaggtag gttcgcccgc gcgcgggttt tgcaaaacga aattgtgggc
    21961 ggatttgcgc accctgctgg catcggccag cgggccagcg gccggagatc gggcaaaagc
    22021 ggctcgtttt gttcgacatc ggggcggctc gcgctaaaaa caaggccaac gaatccgcca
    22081 acgcccttga ccgcccccgc cgccgacccg cccggttttt tcctctcctt cgaaggctcc
    22141 gaaggctgcg ggaaatccac gcaaatccgc ctcctccgcg cgcgtctgga ggccgccggc
    22201 cgccgggtgg aggtgctgcg cgagccgggc ggcaccgaaa tcggcgagga ggtgcgccat
    22261 ctgctccagc acgcgcagga gggccgcggc atgtgcgcgg agacggagct gctgctcttc
    22321 gccgccagcc gcgcgcagat tgtgagggaa aaggtgcgcc cgctgctggc ggagggagtg
    22381 tttgtcatcc tggaccgctt tctggactcg acgacggtgt atcaaggccg cgcgcgcggc
    22441 ctgcccctgg aaagcgtgcg cgccgtcaac gccttcgcca ccggaggcac cctgcccctc
    22501 ctgaccgtgc tgctggacat ggacgcccgc gccgcctgga gccgcatcca tgagacaggg
    22561 cgcgagcttg accgcatgga aagccagccg ctcgagtttt tcgagaaagt gcgcgccggc
    22621 tatctcgcgc tggccggcgc cgagccggag cgcatccggg tcgtggacgc ggaccgcgcg
    22681 ccggagaccg ttcacgagga aatctggagc ctcgtgcaaa cacgctgcca tgccctttaa
    22741 agcggacaaa gccctcgact tgctcgcccg cgcgcaccag cacggcaggc tcggccatgc
    22801 ttatctcatc accgggccgc gcggagccga cctcatggac ttcgccctgc gcgtgctgcg
    22861 cctggtcacg ggcagggagc ggcatcactt tgaggacttc cagcgtgaag gcctctacat
    22921 cctgcgcccc gagggcaaaa gccggcgcat caaggtcgga gatgacagct cggatccgaa
    22981 caccatgcgt catttcatcc accacttcca aatgcgctcc gaaagcacgt tgaaagccgg
    23041 cgtcatcgcc gaagccgagc gcatgaacga ccaggcgcaa aacgccttcc tgcgcacgct
    23101 ggaggagccc cctgcgggaa cgctctttct cctgctcacg cacaatccaa aagcattgct
    23161 tacgaccacg cgctcgcgcg tcattgaaat ccccctcctg ccgccggagg gtgtccgcgt
    23221 cttcaccgcc tacgagcagc gtctgctgga gctgctggaa aacctcaccg tccgctccag
    23281 cggcagtctc ggcgccgccc tcgcgctcaa aacggagttc caggacatcc tcgaagctct
    23341 gcgcggcgaa atcgagcggg atcacaagga ggcctttgac aaggagaagg accattacaa
    23401 gcagaccacc gacggctcct ggctgaaagc cagggaagac caggtggatg ccgccatcca
    23461 ggccgaatac ctcggccagc gtggcgcgct gctcgatctg ctgctcgcct ggatgggaga
    23521 tgtggcccgc cagcaggtcg gcggcgaaca ccttgatctg gacgtcttcc gcgaggccac
    23581 cgccaagctg gcgcagcgct ggactcctga ggagacagac cgccgcctgc gcgtcctgcg
    23641 gcagctcgaa tcccacctcc acaccaacgt gaacgagagc ctcgcccttg aggtggcctt
    23701 catccaggcc ttcggaaggt gaatatcagc cagtgcgctc gcagcagatg cgcgccatca
    23761 cggcgctcgg cggcctgcca cttgtcgcgg ttgcattccc attcgatgcg tttgctcgtt
    23821 tcgttgtgac ggcaatgctt caggcaccgc gacatggggc ggcggcgttc ttcgcggtcc
    23881 aggcagcggt aaaggaggtc cttgtgcgcc accgcgaagt cgcaggccag cagcacatcc
    23941 atcaccgtct gtgggaacca tttttcatgc acgaagagtt cactgcgcgg atcaagcagc
    24001 cgattgaccg cgagcaactc cagctccttc gcccagggca cctcgccggg cgggccttcg
    24061 ggaccttccg gccctggaag accaccggca gccgcgtcct ggcccgccgc ggtttccgct
    24121 tccgtttggg caaatcccca cccctcccgc gcgcgggacc gttggcagac ggcagcttca
    24181 tggcaaggca agacctccta cctcggagtt gaagacttcc gggaaattgt cgcacttttg
    24241 ggcgaagttt tccccgcccg ctcaacgcgc ttagaaccag tcttttccga ttcgagattt
    24301 ttagccgcca tgagcgcggg cacgccgccc gtgacgccaa cgagtccgcc gcgaaggcca
    24361 tgggcgacga aaaactcaaa gtcatcgcgc cgaactcatc acccaagtgc gcaagagcgt
    24421 gacgattgac tggacttttt tgcgcgaagg cgcgcgggcg aagattggct ccctcgctgc
    24481 cgccgctcgg tcggcctgcg gcctgtgctt cgctcagcct gtgtgcctca cttcgttcgc
    24541 ccttcggaca tccggtggct ggctatctcc ggcttcgcct tcgtttcagt cgccccgctc
    24601 cttcggctcg ccggacttgc aggaggaagc ggtgaagacg gtgctcattc aggcggagct
    24661 gcttgtgcgg agtgggtttg agaagaattt ccgcagcgaa tgaagaggtc aggtgtgccc
    24721 tgggtgccgt gccgcgcaaa tttttttgca tattttgaag gcaggtgatg ccgccactcc
    24781 gggcagccgc gcgcagtctt ggggtgggga tcactctccc ccgagcaggg cgcgggcctt
    24841 gtcagcggtg acgcccatgc ccgggatttc gaaaccgagg gtgtcgccgt tgggctggaa
    24901 gccgccgccg tccacgtcca cataaatggg gttgttgtag gcgcatgggc gcatcttggc
    24961 gtagtcgctg gtgccgaagc cggtcctcat gtcgccggtc tcgtgcaggg ccactacgat
    25021 gagatgggcg tccttttgca gggggatgcg cagtttgtgg tcgaacttga cgacgccgtt
    25081 ctggaacatc tgcgggtggg tttcgcgggt gaaattgaag ccgggatggg ggcgggagtt
    25141 cacgaggacc tggacgcggt tgatgtccat ccagtcggtg cattgcacgc ggacgtggag
    25201 gtccatgccg ccggttgcgc gtgtgtcgtc gccggcagtt ttgccgtcag gggtggtgac
    25261 ctggaggaag ggaccggtgg tgaggatgat gttgccggct ttggcgcggg gggcgagttc
    25321 ggcccagtct atcttggcgg ggtcgtcggt gctgctgggc aggtagatgc gccaggagcc
    25381 gacgccgttg ccatagacgc tgtgggcatc ggccacgccg acggcggtga gacggtggcc
    25441 ttgattgagg agctggcgcc agaggaactc gcggacatag gagacgcggg tggcgacgcc
    25501 gctgcggctg aggctgaagg gggagtcggc gaggatgctg gtgccggggc cgttctggga
    25561 ctcgctgccg tcaatcatct cccccacgcc gacgaaaccg ccgtcggcga tgccgtcgcc
    25621 gtcgcggtcc acaaacatgt tggagaggtc ggggtggttg aactggatcc agcggtcggc
    25681 gcgctcgccc tgccagcggc gcagggtgag ggcggtgatg cgcgggtcgt cattccagac
    25741 gggcgcgcca ccgtcctgga ggaggggatc aggctcgaag gggaaggcgt tgaagtgctg
    25801 gcggcggccg gtgagttcga cgcctttgac ggtcttgatg aaaggggcga ggccaagggc
    25861 ctggatggtg ggctcccagt caaagaagcg gttgtgctcg gtggtggggg cgaattcgag
    25921 gtgctcggcg gcgatgttga tgaggcggcc gtcggtgtcg cagacgttgt caccgctggg
    25981 ggtgctgtgg ttgtggaagt cgctgctgat ccagccggtg gtgtccacga ggcgcttgag
    26041 ggtgccttgg aagtcggtga tttttccggc ttcgacgctg acctcgcggg cgatgtgccc
    26101 atactcgggg ccgcgcacga cgacgacacg gtatttgccc ggcgggagct gctgggtgaa
    26161 gtgaccgttc tcgctgtggt actggtccac acagccgtgc gcgcgccatt tcgggccgag
    26221 gttgagcttc ggcgcgcctt catccagcgg ctcaaagtgt gccttgcagg gcagatgaga
    26281 gccgtcctct ccggtgatgg cgaagtggat gccggcggcg tcctgcatgg cgaccttcgg
    26341 cgcggtggtg gcgccttcgg agacggtgag actgatttgt gcatcggggc ggcccagatc
    26401 ggtgatggtc agcccggcag ggccgacggg tagtttgagg ctgaatttgc cctcggtgtc
    26461 cgggtaggcg ggcacgctgg ctttgccctc cggcacgagg atgctggcgg tggggatgcc
    26521 tttgccgtcc gcgccggtga tttgtccgct gacggtgccc agcgcgccgc ctttcaccgc
    26581 acggaactcg cccacggctt gcgcggggga ggtgccgacg gcgaagaagc gggcgatgac
    26641 cacctcctgg ccgggggcga gttcgaggct gtcagtaagc ttctcgatgc cggtgaggct
    26701 gagcacgccg aaggcgtagc cgcatttgtg cgcggggttg atggcgtccg cccagcggat
    26761 gccgtcggcg gtggtgccgg tggagtcaaa gcgggtgagg tcgtccttgg tgctgacctt
    26821 ttgcggggcg ccggattcgt tgcgcagcac ggtggtgatg aagactccct gcacgccgtc
    26881 tttgacgcgg tattcgtggc gcttgaaaac gccgccggag agcgcggagg tgatcacact
    26941 ttccacggcg gcggcgccct cctcccgcgt gacggcgggg cgcacatagg agacggggcc
    27001 gtggccgcag ggaccgtagg ccacgagctg gtcgttggcc tggccgcgca gggtgagatc
    27061 atagagcagg ccggggctga cgccgtcgtc gccatagaag gtgctcatgt tggcgcggcg
    27121 gttgggcgcg ttgtgggaga tgagggcctc caccttgtcg ctgcgcaaga cgaagtctcc
    27181 ccggatgccg tcggcctcct tgccgcctgg caggtctttt tcacggtcgc cacgcgcctc
    27241 gaagacttcg gtagcgtgca tgctgagcgg gagaaggcag aaaagggcgg ctgttttcat
    27301 gatgcgggct ggcggactac cacctacgcg tcatgaatgc cgcatcattc tttcgtggcg
    27361 ggtgaggcgg gtgtttcggc ggcattcggc tgctgcggag gccgggcgct ttttttgcgg
    27421 taatcgccgg gatagcgttc cttcacctgc ggcggcatcc ggtttggatt tcccagcacg
    27481 gcacggccgt ccttggtgcg gctgacaagg ctgccgctgg gcagttccag ataggcggcc
    27541 acctcctgca tgcgcacggc ctcctggggt gtgggggcga tctgtgacgc ctcgcgcagc
    27601 atgggcgctg cggcggcatc tccgagcacg atcatgccat tgatggcggc ttccctgatt
    27661 tcgggatctg gatggctgag aaagggccgg atgcgcggca ggtccttggc atcgtaggta
    27721 atggaggcct gctcgatctc ctgtaggatc acttcgtttg ccgcgtccat cggcacggtg
    27781 gcaggcgcag gggtatttgg ggaaggtgca acggggttcc ctggattggc tccacccgaa
    27841 gcgcccggtg gcagggattt cgtttccggc acgggtggag aggcggcctg tgcctcatcg
    27901 gggccggctg cctggaagga ccagaagacc accgctgcca gaaggcagcc gccaacgatg
    27961 aatatccagg ttttcatggt tgaaataaaa gcgcacccgc ctccagagga tgacgggtgc
    28021 gttaaggtca gccaatgtga tcaaggatca acgggcggga agaggaaggc gtcaatgtca
    28081 ggtatcacct tgccgccagc agcgctcaga gtgccttcga tgacgccgct gaccggatta
    28141 agagtggctg ggtcgaatac atttggcaga gtgaagatgc cggaaatggc cgagaccttg
    28201 gaggctcccg gcacatagac ggggtatttg aagctggttc cgtcaaagtc cttgatgttg
    28261 ataaaggtca gcttgccgcc gcctgtgagg atgccgtgac cgatcaaggt gtctcccatc
    28321 cagatgaaag agactttttc tttgtatttg taaacatcag tgacggtttc tgtctcgatg
    28381 atgtcctcag gcttgaacac cattttattc acatacttga aattcacatc ttgggcatag
    28441 ccgcccagag tgggatcttc accgaatggt agctggatca aaggtgtgat gtccacgact
    28501 ttgcctgagg tgtgcctggc atagagtaca gagaaatcat cgaactcatc cgtcacgacg
    28561 atcagcgacc agccagcgat gaaaggcgcg ttgccaattt cttcaatggc tcccgccgcg
    28621 acgagatcga ggaggatttc cttgttggaa tatttgcggg tggccatttt ggagatgaac
    28681 tccgagtggg cttcgtcgct gatgggatca ccggcggggg tggatttgct gataaaccag
    28741 tcgttgaagt aagtcgggcc gccgagagga tcgccaaatt catccaggac tggcaggggc
    28801 ttgccggtct cggggtcttt gagaacggtc cccggcttgc tatgagtcag ggtgagggct
    28861 gccgtaaagg caccgtaggt gcctggcact tgggccaggg tgactgagca agctccaaaa
    28921 aggagcccga ggatcgcaag acgtgggagc gctttcatga ttcgtggtgt gtgttgtttt
    28981 tcggcgggaa tattccctgc atcatccatg taccgatttc gcgcacgaat gtcacaaaaa
    29041 attttcacag agtcgcaaaa acatctcttg catgagccgt gcccgctggc aaaacctgaa
    29101 agagccaccc caggggccgc cggatcacag ccgaaggaag gcgcgctggc ggtagagcca
    29161 caggcagagc agccagagaa cgagcagcat gccgccgcgc tccagcacgg gcgcccattc
    29221 gccgctgaac cactgcgcgc cgatgtgggt cttcaagcgg cctgtgatcc agcccgcgcc
    29281 gagactgtgc atgaggtaga tgaagatgct gttcatgccg acgacgacga gcgggaagac
    29341 cagccgccgc tgccccgcca cctcgacgag ccagtaaaag gcggcgagca tcatgagcac
    29401 ccagccgccg ctgaagatca cccacgaggg cgtccagatg cgcttcacgg aggggacgag
    29461 gaagatgtcc agcaaagtgc cgatgagcag gcacaggact ccggccacga gcaggttcac
    29521 gcatttgcgc gcatgagtct ggcggctgcg cagcagcagc tccgccgtca ccacgcccat
    29581 gagcatggtg gccagggcgg ggatgaagtt cagcgtggtg tagccgccgg cattggtgac
    29641 gaagggctcc ttgcgcggga acaggttcaa aaaccaccgg tcaaaatccg ccgccgcgtt
    29701 caggtgcttg ctccagtggc ccgacgcgcc gggcagcagg tcggtggcgg cggcgcccac
    29761 tgaggcgaag tcgaaatccg cgcccggcag cggatgctgg acgaaccacc accagactcc
    29821 cgccaggatc accaccaccg ccgccgcgca gtattcccag ccgcacacgg ccagcagcca
    29881 gaggaagacg tagccgaggc cgatctgcgc cagcacattg gtgaactgcc agttcgtctg
    29941 cgcgtccccg tcccgcgtgg cgagaaagac gccgatcaaa acgagcgcca gtgcgcgtgc
    30001 gatggcatgg ccgaacctgc cgaaaaagcc ctggccctgc tccttgcggc gcatcaggga
    30061 gaagggcagc gccatgccga ccatgaacat gaacgagggc tggatcaaat cccacagcgc
    30121 gcagcccgtc caatccacat ggctgacctg ccagccgatt tcctgccata gacgggaatc
    30181 cggctccgtc ttcgccatct gcgccaggcc gagaccggag gaggccatga ggagcatgat
    30241 gaagccgcga aaggcatcca gggagacaag gcggtcggga ggtggttgca tgggccgctt
    30301 tgtacgcggc gccgggcgcg cggctatctt cgagactcaa aggcgcgcgg ccgccggcgg
    30361 cgcggcgagc agcgccgagc aataccacag catgcgcggc aggtggaagg gacccttcca
    30421 caggctgccc ttcatgcgtg tggagggagt gccatccctg cgcagccagc cataccactc
    30481 gccatgctcc gggtcgggaa agcggctgaa gctccattca tgcgcctgcc ggtgcatctc
    30541 cgcgtacttc ggctcgcctg tgatctcatg cgccagcgca gcggcgatga cggcctcgca
    30601 atgcggccac cagaacttca tgtcctgcca gtactcggcc accggcttgt caaaaacatc
    30661 acggtaataa aagaagccgc catgctccgc gtcccagccg cgccgccaca tgcaatccag
    30721 gatgcgcagg ccgaggcgga tgtaggcccg ctcaccgcgc agccgcccct cgtgcagaat
    30781 gaaccacgcg cactcgatgg cgtggcccgg attcaagcag cgcccgtcga agtggtcaat
    30841 gatgccgccg tccggggcca ccgtctccat gagcacgtcc atgtcctctt tgaaaaacaa
    30901 acgcccaatg tcaaagatga acttgtctat ccattccgtg gcgctgcgtc cggccacctc
    30961 cacatggccc agatttgccc gcaactcctg cgccgtggcg atgccgatca tcggcgggcc
    31021 gatgctgatc atcgggcgcg tttgctcatt gatcttcggc gacatgcccc ccggctcgaa
    31081 ggccagcctc aggaacgacg cgaagtcccg caccgccgcc tcgtggtagc ggtggtcccc
    31141 cgtggcgcgg gcataggccg cgcgggcgat gcaggcaaac gtctcgctgt aggcgtagcg
    31201 cctcatgcgc aggggcagcc cctcgcgtgt gagggtgaag aagagtttgc cgttcgtggc
    31261 atggccgtgc ttgtcgagaa aagcgatgca cgaccccgcc gcctcgatcc agtcccgccg
    31321 cttctccacc gtgttgtaca gcgtggcaaa catccatccc gcccgcccct ggaaccacac
    31381 gctcttgtcg ctgtccagca ggctgccgtc ccggtccagg caggtgaaaa tcccgtcatg
    31441 ctcccggtcc atgccgtggc gcagccaaaa gggcatgaca ttttccacca gctcggagcg
    31501 gtaaaaggcc gagagccgcg cgcgaccttc cggctccgtc aaaagagggc atgggatcat
    31561 gccccgcatt gaagcccgcg cgcggacgat gggaagcgcg ggaagcggaa aaaacacaca
    31621 cggcatgcgt ttcgccctgg actcttgagc aaaaccactc accaatttct tttggcgagc
    31681 ggtctggatt aagagccgcg gcggcttcct caacttaagt cttcgctctc gccaatttct
    31741 tttggccatc cagatttcta gtgacgcttg agacgaaaaa gaaccatctt tttgaacatt
    31801 tgcgatacct ttttatgctg gtgcggcagc ttttcctgcc tctgctatcg ttgcagtgag
    31861 ggataaagcc gctgcagcga tcaaaaaagc cgccgcagtc atgcaaaaag ccgctgcatt
    31921 ggttgcaaaa gtggtttgat gcagggttca agctggcgta gatgcccaaa tagcccttgc
    31981 agcgctcttt caagctggcg cagagtcatt tgcagggctt gcacgcctgc ccggagccgt
    32041 tgcagatgcc gtggtagagc cgagtcgaag ggcaaaagat gcgcacacca aagcggcaaa
    32101 aagcgggctg cattttttga ggcgcgtcgg gcggaccgct gccggctcca ggatccgtgg
    32161 cgtcacctgc atctcagcga caggcgcgat ccatgagcct gccgggaatg cgcggacatg
    32221 ccccgacaag cttcaccggc aacccgtccc ggagttgccg gagaaagacg ggggtgatgc
    32281 atttttgtcc ccttcctggc atggggagtt ggaacgaata caaaaaactg acagattttg
    32341 ttatgagaag tatgatcttt tattgcatta ttaaaaaaat tcgcattttt gcttctcatg
    32401 tctggcgcta tccaacgcat ctggctgcct ctgctggcca gtctgctccc tgctctgtcc
    32461 gcaggagcgc aggtgctgcg tctggaggtg gcaccggttt gggagaaaag tgagttgaag
    32521 tcgaaccact ggcagccgga gggatcgccg gctggtcttt ctgtaaaccg tttggacttt
    32581 ttgctttcga agctggccct gcgtcgtgcg gacggctcat ggctggagtc ggaggactgg
    32641 caccgctttc tcagtctgga aaaggggctg ctgacggcgg aggcggaggg gttgccgaaa
    32701 gaggacttca ccggcatccg cttccaggtc ggcgtgccgc cgaaggagaa cgcgggagat
    32761 gccgcgcagt ggccgcccgc gcacgcgctg aacccggagg tgtgcgggct gcattggggc
    32821 tggcagggcg gctatgtgtt catggcgctg gaagggcgct ggcggcgcgc ggacggccag
    32881 ctcggcggct tttcctacca catcgccaat gatgaaaacc tgacgacggt ggaaatgccg
    32941 gtggacttcc gcggcggcgg cccggtgacg atccgcctgg cgctggatgc ggcagtgctg
    33001 ctgggcggcg tggatttcct gagggatggc gactcgacgc acagccgcgc gcgagatccg
    33061 ctgcttgtga agttcacggg caatctggcg cgcgccttcc gcgcgcagtc ggtgagctat
    33121 gacctgtttc aatcacctgc ggtgacggag aaagcggccc cgccgccgcc gggcacgacg
    33181 ccttttcccc tggcggtgac gcagcgcttc ccgcaggcgg ccttgccggc ggacaatccg
    33241 ctgactaaag aaggcgtgga gttgggaaag cggctgtttc acgacacgcg cctctccatc
    33301 aacaacaccc aaagctgcgc gggctgccac agccgcgcca acgcgctgac cgacccgcgc
    33361 cgcgtgagcc tgggcgcgca ggggcaggcg gggaagcgga atgccatgcc gctttttaat
    33421 ctggcctggg cgcgcgagtt cttctgggac gggcgggcga agacgctgcg cgagcaggtg
    33481 ctcatgccca ttcaggacgc gcacgagatg aacgagacgc tggacaatgt ggtggcgaag
    33541 ctggcggacg agggcgcgga gtttgaccgc gctttcgcgc cgggcggcgt gacgacggag
    33601 cgtctcgcga aggcgctgga gcagtttttg ctgagccaga tttcgcaaaa cagccgcttt
    33661 gaccgcgccg cgcggaaagt ggcggagctg acccaggagg aaaagcgcgg cctgcagctc
    33721 ttcgtcaccg aatatgaccc ggcgcgcggc cttttcggcg cggactgctt tcactgccac
    33781 ggcggcacgc tgttcaccga tcaccagttc aaaaacaacg ggcttgatct ggagccggct
    33841 gatcggggcc ggatgctcgt gacgggcgac gcggctgaca agggcaagtt caagacgccg
    33901 agcctgcgca acatcgccgt caccgcgccc tacatgcacg acgggcgctt cgccacgctg
    33961 gaggaggtgg tggagcacta cagcggcccc atgccgagga gcgacacgct cgatccgaac
    34021 cttgccaaac accccgacgc gggcatccag ctcagcgccg ccgacaagcg cgccctggtg
    34081 gcctttctga aaacccttac tgacgacgac ttcatcgcca cccccgatgg cgaggtgacg
    34141 cagacgaacg ctccatgatc tcccggaatt ttttctccca caggcgcgct tcccaaagcg
    34201 cgggtttgac tcccgcccac acctccagcc cccgcatgga gccaacccga aattcgtgtc
    34261 cattcgtgtc cattcgtggt ttcctcctgc ccttttatct gaccgcgctt ctcgcgcacc
    34321 ccgcacaagc cgatgacgag catcagatag acgagcaact gctcgccgag gccaacgcgc
    34381 tggcgcctga attccaggcg gcgcaggtgg aggcgcccgc gccgcacgag ctgtcacttc
    34441 aaggccaatg gggtcccgtc attccatgga cgccgcacat ccctgtcacg gcggcaacgc
    34501 tgccggacgg gcggctgctg acgttttcct ccaaccagcg caccactttc ccgagcggtg
    34561 tcgagttcac ttacgcggcg gtgtggaacc ccgccacggg cgccttcacc gagatcaaca
    34621 acaaccgcca cgacatgttc tgcggcggca ccgtgatgct gcccgacggg cgcgtgctca
    34681 tcaacggcgg acgcaacacc acgcgcctag gcagcatctt tgactggcgc acgaaccagt
    34741 ggagcgccat ccccaacatg aatgacggcc gctggtacaa cacgagcgtg gccctggccg
    34801 acggcagcgt cttcaccgtc acgggcgatg gcggcagaac caccgccgag cgctggacgg
    34861 catcgaacgg ctgggcgcgg ctgggcggca tcaactggtc ggtcgtcgtc aaccagccgg
    34921 gttatgtgag caactggcat ccgctcgtgc tcgtggcgcc ggacgggcgg cttttccacg
    34981 gcggccccac gcgccagatg aactgggtca ctgccaccgg caccggcggc ctttcctacg
    35041 ccggcgtgga tgtgcccggc gcgttgtatc cgaaggaggg ctgcttcgcc atgtatgacg
    35101 aggggcgcat cctcgtggca ggcggctcct cgacgaccac ggccaacagc ttcgacagct
    35161 ccaccggcac cagcacgaac gccgccttca ccctcgacat ccgcgccggc gcgcccgtgg
    35221 tgcaaatgac ggcctccatg cgttacgcgc gccagtttgt gaactcggtc atcctgccca
    35281 atggcgaggt catcgccatc ggcggcaaca cctccggcag gaaattcaac gacaccggca
    35341 gcatcctcat cccggaaatc tggaatccgc agaccggcca gtggcgcgag gtggcggcga
    35401 tgagcgtgcc gcgcaattac cactcgctgg ccctgctgct gccaaacggg cgcgtgtggt
    35461 ccggcggcgg cggcctctcc ggcaacagcg ccgaccaccg cgacgcgcag atctacacgc
    35521 cgccgcagtt gttcgccgcg gatggcgcgc cggcggtgag gccggtcatc tcgcaaaagc
    35581 ccgcctacat cgcgccaggc agcgtttttc aagtcaccgc cagcaccggg gtggcgcgct
    35641 tcagcttcat caagatgtcc tcgcagacgc actcggtgaa caccgacctg cgctttctca
    35701 acgtgccctt caccgggacc gcgcccggca cctacgcgct caccgcgcac agcaatgaga
    35761 acgtgctcac tccgggttac tggatgctct tcgccctcga cgcgcaggac gcctggtcgc
    35821 aggccgccat catgctcgtg gatccgaacc tgggagtcac catcacgaat cctggcgcgc
    35881 agatcagcgc cgtcggtgtg gccgcctcgc tcaccgtcct ggcaagcgcg cccggcggcg
    35941 gcggcacggt ctccttttcc gccgccggcc tgcctccagg cttgtccatc agcgcgcagg
    36001 gagtcatcag cggcacggcg tcgcaggcgg gcgagttcaa tgtcagcgtc accgccgccg
    36061 atggctccac caccgcctcg gcggagtttg tgtggagcgt cacgccggcg aacctttatc
    36121 aaaacatcgc tgatttctcc ggcacggacg ccatgctgca gcttaacggc gtcgccgcgc
    36181 tcaacggccc ggtcctgcgc ctcacgccga acgcgggcgg acaggcgggc gcggcttttc
    36241 tgaaaagcgc cctgcccgtg tcggcgaaca cctccttcac cacgcgcttc gtcttccgca
    36301 tgagcggcac ggcggacggc gcggacggca tggcattcgt cgtgcagggc gaccgcgcca
    36361 ccgcgctcgg caacagcggc agcggcatcg gttatgaaag cgttttgaaa agcctcgccg
    36421 tcgagttcga cagctacgcg ggttcgggtg atccgaacgg caatcacgtc ggtgtgctga
    36481 ccaatggcaa tgtgaccagt catctcgtca cacacacgcc cgcctttgac ctcgaggacg
    36541 gcagcagcaa caccgcatgg gtggagtatg acggcgtggc ggacacactg cgcgtcttcc
    36601 tggcgcaggg tgtggtgaac tcgcgccccg ccagcccggt gatcacacgc gcgggtcttg
    36661 acttgcccgc gctcaccggc ggcgtggcct gggtgggatt cagcgcgggc acgggaggtc
    36721 tggccaacaa ccacgatgtg ctcgcctggg agttcaactc gaacgccttc accctgccgc
    36781 aacctcccgt gctgacgaat cccggcgatc aaaccggtgt cgtcggcatg gcgctcagcc
    36841 tgcccgtcca agccagcgat gtgaatctcg acacactcgc ctacagcgcc acgaatctgc
    36901 cgtccgggct caccatcaac gccgccaccg gtgtcatcag cggcacaccc gcaacggcgg
    36961 gcgcggggat tgtcaccgtg actgtcagcg acggcaacac tgctcccgtg agcgcggact
    37021 tcgagtggct gattaatccg ggcctcgaac tccagcccgt catcacccag ccagcgcctg
    37081 ctggaaccag catcacctac accgcgcaga gcaccggagg cgcaaacccg cgctacaagt
    37141 ggagctttgg cgacggcacc atcgagacgg ccttcagcgc aaatcccgcc gccacacaca
    37201 cctttgccaa tcccggccgc tacctcgcca ccctcaccgc caccgatgac accggccgcg
    37261 tggtcacacg ggacttctgg caaaacatcc acgcccctct caccgccgag cgtcccgccg
    37321 tgtcgagcgc catcgcttgc gagccgcgtg ccacgggcaa cgcgcgcgtc tgggtggtga
    37381 acccggacaa tgacagcgtc gccgtgctgg atgccgtcac gcgcgccaag ctcgccgaga
    37441 cacccgtggg caaagccccg cgctctgtgg ctgtggcacc ggatggccgc gtgtgggtgg
    37501 ccaatgtcga ggacgccacg ctttccatcc tggacagctc caccttcgcc gtggcgcaga
    37561 ccgtgacgct cgcgcgcggc tcgcggcctt tcggtctggc cttcgcgccg gatggctccg
    37621 ccgctttcgt ggcgtgtgaa agcgccggcc ttttgctcaa gctgcatcca accaccggcg
    37681 cgcagacagg ctcctgcaat gttggcgctc atgcgcgcca tgtctccgtg gcggcggaca
    37741 gcgcgcgtgt ttatgtgagc cgtttcatca ctccgccgct gccgcaggaa aacaccgcca
    37801 gcccgcaaac aagcgggcac ggtggcgagg tggtcgtcgt caatgcctcc gccatgaccg
    37861 tcgagcgcac cctgctgctc gcgcacagcg aggccgccga ctcgcccact tcggcgcgcg
    37921 gcattcccaa ctacctcggc gcgcccgcca tttcccccga cggcctcagc gcctgggtgc
    37981 cctcgaagca ggacaacatc cgccgtggca tgttccgcga gggctcgcag ctcaatcatg
    38041 accaaagcgt gcgcgccatt gccagccggc tgaacctcgc cacacaaacc gaggacctgc
    38101 ccgcccgtgt ggactttgat gatgccggaa tgcccagcgc cgccgtcttc gatcccttcg
    38161 gcaatctttt gttcgtcgcc ctcgaaagca accgcgccgt ggccgtggtg gacgcctggt
    38221 cgaacgagga gctgctgcgc tttgagacgg gcctcgcgcc gcagggcctg gcgctctccc
    38281 cggatggcgg cacgctcttt gtgcagaact tcatgagccg cagtgtgacc gtgcatgacg
    38341 tggccggcct gctcgcgggc agcggcgccg cgcccgcgct gcttggcacc gtcggcaccg
    38401 tggccaccga ggcgctgccc gcgcaggtcc tgctcggcaa gcagcacttc tatgacgccg
    38461 ccgacacccg cctggcgctg caaaactaca tctcctgcgc cgcctgccac aatgacggcg
    38521 gccacgacgg ccgcgtgtgg gacctcaccg gcttcggcga gggattgcgc aacaccatca
    38581 ccctgcgcgg ccacggtcag cacggcgcgc tgcattggac cgggaatttc gacgaggtgc
    38641 atgacttcga aggccagatc cgcggcctcg cgggcggcac gggcctcatg accgacgcgc
    38701 agttcaacac cggcacgcgc aacctgccgc tcggcgatcc caaggccggt gtcagcgccg
    38761 atctcgacgc gctcgccgct tatgtcacct cgctcaccag cgagtcgaaa agcccgcacc
    38821 gcgccagcaa tggcgcgctc accgcgcagg gtgccgaggg tgaaaaagtc ttccgccgcg
    38881 agaactgcgc ctcctgccat gccggggaaa atttcaccaa cagcgcgctc ggcgtgtttc
    38941 gcgatgtcgg cacgctcaag ccctccagcg gccagcgtct cggtgccgcg ctcacgggtc
    39001 ttgatgtgcc cacgctgcgc ggtgtgtggg ccaccgcgcc ctatctgcat gacggctccg
    39061 cgcccacgct cgccgccgct gtcagcgcgc acagcggcgt cacgttgagc gtggcggaca
    39121 tggacgcgct cgtctcttat ttgaatcaaa tagacgacca gcccgccggc gcgcccgtgc
    39181 ccgtgaccgt ggtgctggaa agcgccgcgc ccgcgccggt cagcgggcct ttcaccgtca
    39241 ccgccacctt cagccatgcc gtcaccggct tcacgctttc ggacatcact gtcaccggcg
    39301 gcagcgcctc ggcgctcaca ggcggcggtg cgaactggag cttcaccata acgcctggcg
    39361 cggacgtgtc cgtgtcactc gcggcgaaca ttgcccagga cgccgccggt cttggcaatg
    39421 ccgcctcgaa tgtgctggca cgcatttatg gcgcgcccgc ccccgtcttg atcagcgagg
    39481 acatcggcaa cgcgcgcgtg gctggcgtca cagcgcatga caccgccacc ggcacctaca
    39541 cgctcacggc ggatggcgag gacatcttct tcaatgccga cggcttccac tttgccaaag
    39601 tgctgctcac cggcgacggc gagattcgcg cccgcgtgcg cagcctggac aacacgcacc
    39661 cctgggccaa ggccggtgtc atgatccgtg aaagcgccgc cgcgggctcg cgccacgcca
    39721 gcgccttcat cacgccgccc gcagcgggca acggtttcgg catggtctgg cgtccggcgg
    39781 ccaatggcgc gacgacttac gcgggcggcc cggctttgaa cgcggtgccg gacaactggg
    39841 tgcggctcgt gcgcgcgggc aatgtcatca ccggttacgc ctcggcgaat ggcagcgcgt
    39901 ggacgcagat tcagtccgtg accctcacct cgctggccgc gcaggtgcag gtggggctgg
    39961 tgctcacttc ctccgacatc tcccagcgcg ccaatgccgt ctttgattcc gtggaaatcc
    40021 tcggttccca agccgtgagc gcgccggagg cggtgctaag cgcggcctcg gccatcgaga
    40081 ccggagcctt caccgtgcag gcgcagttca gccagccggt caccgggttg gagcttgcgg
    40141 acttcaccgt cacgaatggc gccgcctcga atctcaccgg cagtggcgcg gcttacgcgg
    40201 tgacgatcac gccgcaaagc gcgggcctcg tcaccgtggc gctgccagcg ggcgcggtgg
    40261 tgaacgcggc ctcggtgccc ggcacggcat cgaacacggt catggtgaat tacgcgccgc
    40321 ccgtggccgc caccctgcaa ggacaggacg tgggcgatgt cggcgcggcg ggctcgacga
    40381 gctacgatgg ctcgctttac accctgcgcg gctcgggcga ggacatcttt ttcaatgccg
    40441 acggcttcca cttcgcgctc acccagctca gcggcgacgg cgagatccgc gcccgcgtga
    40501 cgagccagac gaatcaaaat ccctggggca aggccggcgt catgttccgc gagacgctcg
    40561 ccgccggatc gcgccatgtg atgatgttca ccacgccgac gggcgcgggc aacggctttg
    40621 gcatggtgac acgcgccgcc acgaacggcg cgacggctta caccggcggc ccggccttga
    40681 atgccgcgcc gaacaactgg gtgcgcctcg tgcgctccgg cgccacgctc accgcctacg
    40741 cctccgcgaa tggcacggcc tggaccgtgg tcggcacggc cacgctcagc gggctgcccg
    40801 cgaatgtcta tgtgggtctg gcgcaaagct caggcgtgcg cgcgcagctc ggcacggcca
    40861 cctttgacaa tgttcaggtc gtcggcgcgc agagcgtggt ggcgcctgcg gtcacgctca
    40921 gctcggcctc ctcggtggag acggggccgt ttgtcgcgca ggcgcagttc agtcaatccg
    40981 tgagcggttt gacgctggcg gacttcctcg tcagcaacgg cagcgcctcg aatctcaccg
    41041 gcagcggcgc gctttactcg ctgacgatca cgcccgccac cgagggcgcg gtgacggtgc
    41101 aactgcccgc caatgccgcg caaaacagcg caggcgcggg cagcaccgcc tcgaacgtgc
    41161 tcagcgtctc ttatgtgccg ccggcggtca tttccctggc ggggcaggat gtcggcgcgg
    41221 tcgcggtggc aggctcgacg atcttcgacg gttccaccgg ccagtatacc atgaccggcg
    41281 cgggcgagga catcttcttc aatgccgacg gcttccagtt cgcctccacc acgctcaccg
    41341 gcgacggcga gatccgcgcc cgggtgacga gccagacgaa tcaaaacccc tgggccaagg
    41401 ccggcgtcat gttccgcgag cagctcaccg gcgcgtcccg tcatgtcatg atgttcacca
    41461 cgcccatggg cgcgggcaac ggcttcggca tggtctggcg cgcggcctcc ggcgcggcgg
    41521 cgaactacgg cgcgggaccg gctttgaatg ccgcgccaaa caactgggtg cgcctcgtgc
    41581 gcgcgggcga cagcttcacc acctacgcct ccgccaatgg caccgcctgg acgctcgtgg
    41641 gcaatgtcac cctcacggga atgccggacg cattgcatgt cggcctggca ctcacctccg
    41701 gcagcaccta ccagctcagc acggccacct ttgacaatgt gcagatcgtc agcaccggcg
    41761 cgagcggcag ctcgggcagc agcggcggct ccggcagcac gcccggctca tcgaacaaca
    41821 aggacaccga cttcgacggc gatgacgtga acgacctcat cgaatacgcc atcggctcca
    41881 acacgcgcta cgacgcgggc ctcaccctcg tgagcgatgc cgcaggccgc gtggatgccg
    41941 tgctcgatgt gcttggcgag gccgccggtg tggccttcac cctggaggcc agccccgatc
    42001 ttaccggctg ggtgccgctg ccgctggagc ccgtggcgcg cgatgtggga agcggccgcc
    42061 gccagctcgt ctggaccggc atcagccatc tcaacggcca aagccccgcg cgcggcatcg
    42121 tgcgcctgcg tgccacccac accagcggcg ccaccgccgc gagcacaccc caggcctggg
    42181 tgcggcatga ctttggcgcc ggcacgcaat ccgccggcgt cagcctcgtg cgcgcgccgg
    42241 tctatgccgg cttcatctcc agcctcggcg cggagggcac gctgctgctt gatggcgctc
    42301 tcggcgccgc cgtggacgcg cgggaggagt attacctgga ggtgcgggat ggcgcgctgg
    42361 ccggtcaccg gctggagctt tccctgctgg aggaaggccg cgccgtggcc gacaccgccc
    42421 acacccgagg cacgctggac caacgccagt ccgcgtccgg cctgtccgcc atcacc
//
LOCUS       DAOMYJ010000018        41466 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Prosthecobacter sp. isolate AMR_MDS_5629
            NODE_362_length_41466_cov_22.137693, whole genome shotgun sequence.
ACCESSION   DAOMYJ010000018 DAOMYJ010000000
VERSION     DAOMYJ010000018.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867850
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prosthecobacter sp. (wastewater metagenome)
  ORGANISM  Prosthecobacter sp.
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Verrucomicrobiaceae; Prosthecobacter.
REFERENCE   1  (bases 1 to 41466)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 41466)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.30
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:34:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,742
            CDSs (total)                      :: 4,684
            Genes (coding)                    :: 4,676
            CDSs (with protein)               :: 4,676
            Genes (RNA)                       :: 58
            tRNAs                             :: 55
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..41466
                     /organism="Prosthecobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_362_length_41466_cov_22.137693"
                     /isolate="AMR_MDS_5629"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1965333"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1024
                     /locus_tag="PLP58_03355"
     CDS             <1..1024
                     /locus_tag="PLP58_03355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ07592.1"
                     /translation="VIIHQHGCTNASPDKHPPITGDWHWRALARKHDAALLVPMYHVA
                     KSCDEWNDPDSGSESALFTALEDFAQRSKRPELKDAPWVLWGHSGGSSWSAQMITRHP
                     KRVLAASFRGGCHKQFGVPEFRAKFGPIARDLPLLFVWGKRESVPGSSHFVSWEPMNT
                     MYRELRGQGGKVCRVIDPRSEHGCDDSRFMSINFFDAVLSGDANAAPDENASAPWLPN
                     DTFAALLREFSEIGTLVSAKAPGQAPELQAQREADGKIKLAWRVVPDLAGGLRAIRIH
                     RDGRLWKDLAIKPTDFIATGRDSTPEALRPMELEDESRDTHGYTLTFLDAASHESPRS
                     PPIHVP"
     gene            1098..1874
                     /locus_tag="PLP58_03360"
     CDS             1098..1874
                     /locus_tag="PLP58_03360"
                     /inference="COORDINATES: protein motif:HMM:NF012546.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HRJ07593.1"
                     /translation="MRADIAYKSGSDLSDYERERCKLDLYLPAEKKGFPTLVWFYGGG
                     LTGGSKSGKDTTKIAQSFAAAGIAVVVPDYRLSPKAAFPAYVEDAAAAVAWARQHIGE
                     HGGDPRKLFIGGHSAGAYLTLMLGMDLRYLKKHGIGQDAIAGLIPVSGQTMTHYTVRG
                     ERGQGGRHDVTADDAAPIHHARKDTAPMLLIYADKDMPARAEENEYFTAVLRATGNEQ
                     ITVRKIADRGHGSVGHRIAEPDDPARTALLEFIGMHGETR"
     gene            1953..3485
                     /locus_tag="PLP58_03365"
     CDS             1953..3485
                     /locus_tag="PLP58_03365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ07594.1"
                     /translation="MNPQHSTRRNFVRRFGGTSSQFANLPPALVRLCVCPCVFTALVV
                     HAAEPVEIGRRVQFFMDDHIVDNRWSLKPKTEEVVRAFHAPEKHAANPVIAGQGGYVS
                     AVREPDTGLVRLWYQIAHWTGEAGKGADYAIAYAESKDGVTFERPEFNLVEWQGTKAN
                     NVVWRGPSGRRASGAQIVQAPERDKRGFRYLLTYHTGGAGKGMNGIRVVGSHDGIHWD
                     AGSDSLITELHSDTINSIVHDGDRGQYVMFCRPKDRYLVGGATGNFLETGESRRIARM
                     SGADLWKPWTGPPETILIPDELDMQRGFNRFYGMSARWHEGIYWGALLCFKLNTDITT
                     ELAWSRDGRRFERLPLRPKLLALGPDGAWDGGMVYGSADWVEMGDRWYLYYAGHDGPH
                     ETPHRTPGIGLATLRKEGFVSLRGPRGGGVVCTRLLRWPGGGLKVNADASKGEIRVRV
                     SDEKRRPLDGFGYEDMETFTGDSTALEVEWRGKSLNELQGRAIRLEFHLRNADLFTFR
                     AE"
     gene            3589..4920
                     /locus_tag="PLP58_03370"
     CDS             3589..4920
                     /locus_tag="PLP58_03370"
                     /inference="COORDINATES: protein motif:HMM:NF012754.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ07595.1"
                     /translation="MRTNAVLILVLATVFASSAESPSHIPAFPGAEGAGKWAKGGRGG
                     RVLRVTNLDDAGPGSLRAAVEAKGPRTVVFDVSGTIALKSALSIKQPFITIAGQTAPG
                     DGICLKGHGLYVSNTHDVIIRHLRCRPGDQTSKPGEVDAISMTNVRDVIVDHCSATWS
                     TDECLSITRDSDRVTVQNCLIAEALTSHSLGSIIGSYEGSISFLHNLFASNRGRNPRL
                     SGYQAEAGRGGTSGPRVDFRHNVIFNWYSGPGYTGTAKPDEPERVAFNLVGNHYKPGP
                     ETLASQRALIFTLHEGATVEAYLAGNVNEAASSAAQADLLRIKPGVNLTLRETPVPHE
                     PLERTLTAAEAYASVLQKAGATLPRRDAVDTRIIAGVKDGSGRQIATIPADAWPELRR
                     AAPWTDRDGDGLPDAWEKAHGLDPDDGTDAAHAASSEGWTHLELWLNSPHP"
     gene            4991..6454
                     /locus_tag="PLP58_03375"
     CDS             4991..6454
                     /locus_tag="PLP58_03375"
                     /inference="COORDINATES: protein motif:HMM:NF019113.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HRJ07596.1"
                     /translation="MPLLVPRALRAQNTAEVVRHLPLSISNAPELPLPEGLVTGFGRF
                     GWRSFGVGGEPVVLKDHAGETSIPDGADCRLRLTNAVDVRDDHAFEAATPDGRSLGTL
                     IVRYAALFQVQEMRLTVEQARAVLRQGIVLKMVKGTRPVWFFAPSPEANPSPDALQLP
                     HVLVATAADAAAQFEQRMRGLVMLQGCGWQSGCVSEGLLDLAEKRKDAALFEAVDRYL
                     GMYFTGDGVEFQSPRSARVKDKVGGIEEPLPWATLARRRPDHPALEIGRKFLSSSGIS
                     KDLESPAKELTTEGCYTVAYPMAELARVLKRPELAELALRHLEARKKANVHDGDIYQR
                     AAVEGSKRLRNWARGACWYYNGIVRTLQALDDPAAAKAWQPEIRRVADLLMKHQREDG
                     LWGNFLHDPASAADTSGSAGLAAALARAHASGWLDDKAGQAAQRALKGLAKHLTPDGL
                     LAGAAQSNKGGDALQKSDYRTIYQMGMGLKMQLMAAL"
     gene            6825..7235
                     /locus_tag="PLP58_03380"
     CDS             6825..7235
                     /locus_tag="PLP58_03380"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="sialidase family protein"
                     /protein_id="HRJ07597.1"
                     /translation="MLFSINLLTQQLRAAPLFETQCLFALTPDNRPNCRIPSIIVAPN
                     GDLLVICERRNDGPGDIGNHDIVMKRSGDKGRTWSEEILILDDEDRVCTDITAGLDRD
                     TKTLWLFFLKDKKAVCPSDQHGQRPDVGRAGGDS"
     gene            7225..7770
                     /locus_tag="PLP58_03385"
     CDS             7225..7770
                     /locus_tag="PLP58_03385"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialidase family protein"
                     /protein_id="HRJ07598.1"
                     /translation="MIHEQVVKPEWETLRGRPDTKPHNPKSRAQRWEKDWHQRYGCGP
                     GNAMVQLKSGRLLVPARHREDIGQGRLRSFAHSFYSDDHGATWKLGGNIGLHTSECQF
                     IELNDGSVRVISRNESAEDAPDNLRHLTSLSQDGGVTWSAPQRLEQLITPRCHGAVER
                     HDAKTLLFSAPASPIRQAEHP"
     gene            7789..7989
                     /locus_tag="PLP58_03390"
     CDS             7789..7989
                     /locus_tag="PLP58_03390"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="sialidase family protein"
                     /protein_id="HRJ07599.1"
                     /translation="MRISRDEGATWSAGRTLWPHPGSYSDIAVLDDGSIAVVYERGGK
                     GTTHYWDELHFARFNLEWLEQP"
     gene            8072..9241
                     /locus_tag="PLP58_03395"
     CDS             8072..9241
                     /locus_tag="PLP58_03395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ07600.1"
                     /translation="MSAASAADAGSAWETPPGWTETRGGEGGKVLRVTTLAASGAGSF
                     AEALLAKGPRIIEFDVAGVIDLGGRSLKISNPQVTISGETAPSPGITLIHGGLSVSTD
                     DVIIRHLRIRPGAGKRAKKSGWEVDSLATNSAHDVIVDHCSVTWGTDENLSASGPRFE
                     GATPDEWRKNTSRRITFSHCIIGEGLHDSTHSKGPHSKGSLIHDNTSDIAILGNLYIS
                     NDDRNPLFKGGARGVVVNNVIQNPGSRVMQCSLVESEWKGKEWQRGQMAVIGNVARQG
                     PSTRASVPFISSKGPWDLHLHDNTYTYASGEELSVKASGTNKELRIVSEPPLWPARLK
                     ARPASETAEWVLATAGARPWDRDETDRRLIEEARTGAGKIIHHESEAGASGASRR"
     gene            9283..9840
                     /locus_tag="PLP58_03400"
     CDS             9283..9840
                     /locus_tag="PLP58_03400"
                     /inference="COORDINATES: protein motif:HMM:NF025432.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4256 domain-containing protein"
                     /protein_id="HRJ07601.1"
                     /translation="MKKELSKKQRDELAEVLRARFGKNPHRHQGIEWAQVLARLETQQ
                     GKLWSLGEMEKTGGEPDVVGHDPESGEFHFCDCAPETPKGRRSLCYDRAALDARKEHK
                     PAGSALDLASAMGVEMLSEEQYRELQKLGPFDTKTSSWLLTPPEMRKLGGALFGDSRY
                     GRVFFYHNGAESYYSSRGFRGLLRV"
     gene            10061..11626
                     /locus_tag="PLP58_03405"
     CDS             10061..11626
                     /locus_tag="PLP58_03405"
                     /inference="COORDINATES: protein motif:HMM:NF015585.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialate O-acetylesterase"
                     /protein_id="HRJ07602.1"
                     /translation="MRLPFLILALSVSLAQAELRLPAVISDHMVLQAGKPAAIWGWAD
                     AGDAITVEFAGQTKTAAAGVDGRWSLRLEPLTASAQPRALRVNDRVIRDVLVGEVWLA
                     SGQSNMAMQINGQLHGKVDNADAEVAAAKFPEIRVFVHDTPFAIYELPVPADEHAQDR
                     PGRWRVCSPETVADFSAMGYFVARDLHRELKLPIGIVTAAVGGTPIEAWTSREAQEVV
                     PELKPLLADWTKRLDGFVPAVAQAKFLDAKAAWLKERAKALKAGQPAPKAPAPFKNIQ
                     VMKPGGLFAGMIAPLAPYTMRGVLWYQGERNAAGPFTGLYGRQLETMIADWRARWQDE
                     LHFAWVQLPAFGGAQKLPSEPNGWGVAVREGQRRALRVPRTSMAITMDLGGEKSGHPA
                     NKADYARRLSTVLLHDVYQKDIALPTGPLFKAATVAGGRMIFSFDHAEGLKARDGKLE
                     GFAIAGADGKFHWANARIENREVIVWHGKVPDPQTVHYAFAANPKGNLVNAAGLPASP
                     FAASAPPAGQAGQ"
     gene            complement(11684..13396)
                     /locus_tag="PLP58_03410"
     CDS             complement(11684..13396)
                     /locus_tag="PLP58_03410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ07603.1"
                     /translation="MKPTPALLAFVIPLSSLVIHAAPPAELTALRGQYDKVLGERVTA
                     PFDAALEALNTKFSSALDSAATAAQQTGKLDDVLAIQDDKKRLAEKLPIPDDGEQTPD
                     ALKSLRAIYRGQLKKLEDTRAAALAGVLPAYQSRLQDLETTLTKAGRIDEAKEVRSYR
                     ESLRSSAAPYPDATPGPDATPGLDATPGPAAPAPPATTGSQAKSDDRSAAETALRLGG
                     QVRMTWESPLINDAAGLPKGRLSVLELRFASLPTALDATATAAFAGMQSLQSFYTNRA
                     VNTDEALAFLATCPELRVVHVENGGPQMKGAWLQHLLSLKNLVSITSIASSQKDASAV
                     TQIQGAEMERVTFRDSAVDDAALAVFATWPKLSRLSLRRTRITDEGTAKLAALKKLDF
                     LDVTETPVTVAGVRPLAKLPLKELGFGPNPSVLDTALPELKLVFPGILQFNYPPGGDK
                     VPVTESQLRALHEAWPRINNLNFPTFSRIEDQALAALAAFTALEELHLTQCQVTDAQL
                     TALLAVKQLRELKLDNTTLGDQGLEQLQGLKPLRKLYISKTSVTDAGVAAFKKKRPDV
                     ELVR"
     gene            complement(13505..17305)
                     /locus_tag="PLP58_03415"
     CDS             complement(13505..17305)
                     /locus_tag="PLP58_03415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ07604.1"
                     /translation="MQLRIALLGLSLLATGFNGLLAAPADYNAISGYGTEIPRLTQAA
                     NDVSIVNIQLAGETTEPDGRIRLSFTASLQNSGDSYYESARIAFDPPGPGWPVEIVDD
                     THVFADIAPQSTVAMPETIVLLAPAADALALKNAVLAGQRLRATATELFQFRLPPVAV
                     DEATGEAYDSATENLLTSGVTLIFSESTPLLAGLQPGTLLMEVPFLHTLPRAFDMPPE
                     GEKPKLIGLAKHVSSSQYVMGASHSIRTDRMVEVTSVTFEGDSSVRVHGQQFGGAPPD
                     WLFSTIFPFWNSQTDPNLRGFVDNLRQGTLSVEAIDAFDPPADGDPAARDPFDPPAGN
                     TAFTYLENLQLEAERNGLLNGDAVRHPRLSELNGFFALHMPINDLKITRGITVDGQVL
                     LQGMKVGIDVRYSGLLPVRVTVRLGSELELNLRVTVNPGTDNNGLAEIEREKQIVDFG
                     LPTIYFSIAGVPLKVQPRFSAKVGVTASVPTQVVLPFTGSYEAGVEWIWDLTKPPGMQ
                     VSSEPFSEHRPLTLSKPQIADSLALNVTAWAEARAALIINDIAGPYIGPRANFNFNVT
                     PFAPDWWEADADVDILSGFSIRLLGLELADPVIATFNGPQLFSTDSDGPATRGSAVGH
                     PPPGPLDPAEGGDVRWAKTLQWNNDGPAPYTAQLVRSKLPGVNGETFLIAPNAFEVAP
                     PVVSLDKRGNPLWAKGVNFLFPRKIAATPDGGVIAYCETTASAQVIARFSQNGTVLWV
                     KGAVAVNDDPGQTTKLTKIASVIIRPTTPGDFEILLVGDVNNDATIFDNDPIVMRFDD
                     EGNALASHRFASHNMLESVRDAILLQDGGLLLAGEARGNSDGTPINAGAEWGGWLMKV
                     DPTDWDVVWSVRSKPAITWTRVVENPDGVIFTAGNFAPIVTADIPAMAIGRYLPDGTI
                     DRMISLCEPTVDSTAFIDGPDKGVRNYPNATEWLEDSGYTPHDLLHTLAWTPNGLAIG
                     GVTGNGDNTAPISALLTEKLSVRWMVTHEGDGSPDATVDMLLEDEGIYYLTLSKSFGN
                     SGGLPILTLAKQPYEGTVPYHPSTHALVKYLQPSVYDLLGGNWTIDVPSPPQGYEETE
                     DFTSRYTLQPLTTLPAPPLPEPSSFQDITGFIERRLHLGNPHAPMNYAQWRDYMQLAP
                     NAAPDADSDGDLVSDWIEYLLAGNPHTPDMFPPAILGISRRPDGGIRLHTLRSHAVGM
                     EYTPMQYVTHLGSEMWQPLGELPEDWQPEPSLPSDPANTRRLHLDLNPDTSPRRFFRM
                     LLEP"
     gene            17396..18127
                     /locus_tag="PLP58_03420"
     CDS             17396..18127
                     /locus_tag="PLP58_03420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HRJ07605.1"
                     /translation="MPGTSSISADRFFPPTAWTLVLEAGGAAPDHAREELCKTYWKPV
                     ALFLQSLGLSEPDAEDGAQEIMAQVFGSEGLKPFSREQGRMRHYLKSAARHYFFNHRR
                     DANTRKRGGGAPTLSLDEMPPVPVSESACDALFDKAWALALCERAMSALEASCTRRGK
                     GPLFHALKPTLTLGEEVKPYAEIGSMFGVTEQQIRIEVFRLRRRLAEFLRAEVAATMQ
                     SGQGAAEVEDETRHLIRALAYEPAS"
     gene            18111..19973
                     /locus_tag="PLP58_03425"
     CDS             18111..19973
                     /locus_tag="PLP58_03425"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF019334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional serine/threonine-protein
                     kinase/formylglycine-generating enzyme family protein"
                     /protein_id="HRJ07606.1"
                     /translation="MNPQADTTCAGCGAPLGKDVLGGVCAACFARGALLPGGGWLQSL
                     PTSDEEASQWPELPDWQILGVLGAGGMGTVFRAQRHADGIIAALKVLDGRWSRDPLMS
                     ARFEAEADALKRLEHPNIVRVLETCEAHDGRLCIVMELVDGCDLGRLMRAGPLGAARS
                     LDIFQKVCAAVAFAHARGFSHRDIKPANILVTGDDGVKLADFGLAKEWTEGTIGALTA
                     TTDQFGTAYYLAPERMISRSPDGPPQDVYALGVLLYHLLAGRMPIGNYTPVSRLAPVP
                     AAFDALIAKALEADPARRIESADALGQAIAKAWRAHENSGQRRQNARRLLLVAAAGVL
                     CAGLLTGGAAWQRSRVPERPVFTPPSAVKAGSYWENSLGMRFVPVPGTRVLFSIWETR
                     RRDVEPFIAVEKATVAASYREVEAERSRKSFNIQTLGLGGRLIAAGSWAEPGFPVTDD
                     HPACFMLPRDAARFCQWLTWKEQAEGRLLPGQCYRLPTAEEWLIAAGGEDTPPRAGNV
                     AGPEARDEFWNPRWPTFDKADAFPRMAPVGSFPAELHGLFDISGNVSEWVLRSEADII
                     KPSWETDALLLGPDFHDGSADVASFRHRRPSPMNLRLPNFGFRIVLEHEITKAP"
     gene            20067..21380
                     /locus_tag="PLP58_03430"
     CDS             20067..21380
                     /locus_tag="PLP58_03430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ07607.1"
                     /translation="MPAHADSQTSQPVRTPAGNARLDRAFAPFDIAVCRAAHAGSRVA
                     LLKSLPVFLYVLGGCLKAADFRVENSTVLTSEGAYHWAQSRPAVIPPGKVIVTTQEIE
                     KRGSHGYRDIYITETTDGAKTWSAPKRIAALNRKRFPDGIERVMGDICPQWHEKSGKL
                     LVTGKCFGFLANANDNKAKDDRSQERVAYAVYDPKTNEWSGMKIMDMPGKDHAGNPVI
                     EPNAGCHQRVDLPNGDVLLPVRYRADPKTRVYTTLVARCTFDGETLKYAEHGSEHTIS
                     IPRGLYEPSVCAFKGRHFLTMRGEKNGHVTRSDDGLNYQPTVEWKFDDGQPLLSANAQ
                     QHWITHGDKLYLTYSRKGANNDHVFRNRAPIFIAEVEPEKLCVIRATEQILMPETGVD
                     LTGGFAPVDVNANETWVISSEMAFPEARKDENNRVLLAKIHWNQP"
     gene            21377..25135
                     /locus_tag="PLP58_03435"
     CDS             21377..25135
                     /locus_tag="PLP58_03435"
                     /inference="COORDINATES: protein motif:HMM:NF024240.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HRJ07608.1"
                     /translation="MKTILPIPVALYSACITVIRIFPVSLALLVCKVSLFPADLLAQP
                     LQTDLCIYGGTPGGIAMAVRAAREGLNVILVNHNDHLGGILSNGLGVWDTLWEGKRSP
                     IYDEARLAIFDHYRTTYGEDSRQYRDALPGKSGHTNGKFEPKVAEKILTELVAREKRI
                     RVITHRVPSAVKRDGALIQSVSFMSLWANVEPDEVQAKIFADCSYEGDLATVAKVPYR
                     IGREARSEYNEPHAGVIYMSPVKAAEAPELARAAELHHQLKLRKFSGFQRIKKPESSG
                     EADGFVQAFNYRTILSSDPANRLPVEKPANYDPEKLKLLEHGSIVSPIPNSKRGWNRP
                     QLVGLQTGYVEADWNGRQKIMDAHWDATMALLYFLQNDPSVEPERQKSWREFGLAKDE
                     FTDNGHRPHEFYVREARRITGRYIFTQHDAMLADGLERAPVHEDSIGVTEWYLDTHAC
                     TPRRIEGALEEGKMMLDVETFPGQVPYLAILPQGVDNLLVPVCLSSTHVAWGTIRLEP
                     TWMNLCESAGYAAALAVQNKITPAALDSDLLLRKLANSHVMLSFFNDVDVSSDDPRVA
                     AAQYFATKGFFADYNARLDEPLTESVRATWGKGLTAMREGTLKPMELMKQVHEAELAS
                     SKRMERTRGEALLGMWKVLPAKKAKSTARVPLKIVPVRSSTAAEVKDKSFDLVVVGGT
                     PGGIACAVRAAREGLSVLLVQHNKHIGGMLTNGLMQWDALYGGPRSPVFNECAKLIEN
                     HYRETYGEGSPQHSQARYTQSHYPMSRFEPSVAEHLFNKLVSAEKNITTLLSHYPVQV
                     KREGALLKAMTLREYGTTKDITVTGGTYVDATYEGDLAALAKVPYRVGRESRGEYGEP
                     HAGKVFTNIESKKGPIDVLEGRLNLHTYAHVQGSIDPTSPFTADGAIQAYNHRFCLSN
                     EARNVRLPTQPPGYNREEYVNYNRKGMGAGALNGKSSFNSAILPGENHAYPEATWPER
                     EKIIARHTNFALGLMYFLQNDESVPEAKRAGFRKIGLPLDEYTDNHNLPYELYVREAR
                     RIVGRYVFTEHDNMASRGMTRTPIHADSIAFTDWSMDSHDCTTDRRPGYDYDGKLILT
                     EESRPAQIPYRCLLPKEIDNLLVPVCLSATHVAWGAVRLEPVWMQTGEAAGFAAALAK
                     KHQSTPGKLDPDLLVRTLVMNRHFVTFFNELQQHADHAAMPAAQYFGTKGFFSDYDAR
                     LDEPLTEGVRAAWSQRLKAVLDAGITISKLREQTRGEALLDLWKSLAD"
     gene            complement(25132..26103)
                     /locus_tag="PLP58_03440"
     CDS             complement(25132..26103)
                     /locus_tag="PLP58_03440"
                     /inference="COORDINATES: protein motif:HMM:NF014237.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HRJ07609.1"
                     /translation="MTFVSLSLKWLAAAALIGLSPMCATAAEPLVGVYGENSMQRVLD
                     FEAWLGRPVDVILCTVDFQKWENYRYADWLSKTVYGARGQRRLVYDVPVIIQGASYAE
                     AQKGAYDEHWACLAKSILQNNPGTHDVVVRPSHEMNGDWFVWGVGLSRLAQVPDFIAS
                     WRRFHGVFRGVPGGERFRFSFSASEGASDPRPMWPGDAYVDLVGYDVYWKPKAMGGEG
                     WETNDPREAWEKRTSEHGYNAWNLTGMLAFAKARGKSFQIDEWGVWGPDAAPFVESMA
                     AFIRAYGIRAHTYWNSDAAYPGELHQRDQSWPATSAAFRSSFSKAAR"
     gene            complement(26322..26813)
                     /locus_tag="PLP58_03445"
     CDS             complement(26322..26813)
                     /locus_tag="PLP58_03445"
                     /inference="COORDINATES: protein motif:HMM:NF022182.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjN domain-containing protein"
                     /protein_id="HRJ07610.1"
                     /translation="MSALHAAALKAFKAQGWSHTEVSGMEVVEMAFEAHHAKIPVHAQ
                     SYGDAGIISVVAQAAMPVPRTHRLPAAEMLMRVNKELNVGNFELDWDEGRVMFRVTNI
                     FPTGHYDENILAGLVHLAIAEMDRLTPFLGEICRVSKSELPLLRVPDLLRRSDLLPPP
                     LEE"
     gene            complement(26852..26925)
                     /locus_tag="PLP58_03450"
     tRNA            complement(26852..26925)
                     /locus_tag="PLP58_03450"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(26889..26891),aa:Pro,seq:tgg)
     gene            complement(27109..27783)
                     /locus_tag="PLP58_03455"
     CDS             complement(27109..27783)
                     /locus_tag="PLP58_03455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ07611.1"
                     /translation="MSSRFILPILALAFSGSLHANPEILDAAARQFGGTVRENAVLLR
                     GAGAVSDPVEWTVYSRDAFRPKDILRMRVRLEGPAWVASADGAGSRILDRVPPRSLDS
                     SRIRVRSSLARATAAKAAALAKATFVSVDYQLAANAETGAPEWGLALLDETGYETGFV
                     VVSAENGAVLFQDWTPRVRAAADSQPDSEGERAARSVKRTARRAWNWTDNARRQTGEF
                     FRELFR"
     gene            27929..28957
                     /gene="ilvC"
                     /locus_tag="PLP58_03460"
     CDS             27929..28957
                     /gene="ilvC"
                     /locus_tag="PLP58_03460"
                     /EC_number="1.1.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009958411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HRJ07612.1"
                     /translation="MPAKIYTEKDASLAPLKGKTCAVIGFGSQGHAHALNLKESGVNV
                     IIGLYAGSKSRKVAEERGLKVYDTGEAVKLADVIFVAVPDTKIPACYKADIEPNLAKG
                     KTLLFSHGFAIHYKTVVPPADVNVIMVAPKGPGHIVRRQYTEGKGVPSLIAIHQDPSK
                     KSKQIALAWAHGIGGTRGGVIQTTFKEETETDLFGEQTVLCGGCSALVQAGFEVLIEA
                     GYSPEMAYFECLHELKLIVDLMNESGIAGMRFSISETAKWGDVKVGPKIIDASVKKRM
                     KAALKDIQTGKFAKEWVKEYEGGYKEFNRLLKEGEKHPIEKTGARLRSLMPWIKKRDT
                     KGQQASYE"
     gene            29337..30269
                     /locus_tag="PLP58_03465"
     CDS             29337..30269
                     /locus_tag="PLP58_03465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ07613.1"
                     /translation="MPRFPLHDLSDTEFEALVVLICRELMGIGITSFAPGRDGGKDAK
                     FEGTATAFPSTTAPASGKFIVQAKHISSPVASCSDYDFETTLIDKEIPKITRMLEEGC
                     LTHYLIFTNRRKTGGAEDRITAHIKTATGVQHVWLRGLEDIERELLLQPHIAKAVGLD
                     KLRSPIQFVPEDLRDVILALHAHRQSLPSAFDSEHDFRDYPGLDAKNEVNGLTQAYDD
                     YIKENSMPHFHAIEMFLKNPRNETLKERYHTVADELKGQLIVHRERFLNFDDALETLY
                     NLIHERCPEIQSVRRIVKIIIHYMYVNCEIGQKA"
     gene            30266..30520
                     /locus_tag="PLP58_03470"
     CDS             30266..30520
                     /locus_tag="PLP58_03470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ07614.1"
                     /translation="MIRPAKHLNLNTCVLRAASRLLARLQRERICRFTDLRATLADLG
                     DDAEVVFLPTIHFLYLVGRLDYHPQTDSFEYLQPRKGDAT"
     gene            30517..32307
                     /locus_tag="PLP58_03475"
     CDS             30517..32307
                     /locus_tag="PLP58_03475"
                     /inference="COORDINATES: protein motif:HMM:NF021594.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2326 domain-containing protein"
                     /protein_id="HRJ07615.1"
                     /translation="MKLSHLYSDQPELFPPIRFRDGLNVVLAHIRHPKDDSKLGHNLG
                     KTLLIDVLDFCLLKGINKDHLFKRRTDLFARFVFFLEIQLPDGSHLTIRRSAAETTKI
                     AFKRHEQAWQNFSGLPESDWDHWRETFDNAVNLLDSLLAFTPIKPWPYRKGIGYFLRG
                     QNDYGNVFQLTKFGAGKHKDWKPYLARVLGFDDALLTEKYEADAASTKLHADAAELQA
                     EITLKPKDFDKLRASIAVKRDEVETKLRALDAFDFHGQETDLAREAAETIETEIAANN
                     SLLYNARHDLAQIERGLEDDVHFDLADIQRVFNEAQLSFPDQLVRDYNDLVEFNRRIL
                     TERQTALRDRAAALRAEIAVVEQANAALSNRRREIMRVLGGTDSLQKFKDLQRQTDLD
                     RANLTLMETKAAKLESLLALQDKLRQTKARAEELTAAIRQMVHQGSARYQEIQRTFSR
                     IIKEVTHRTALLYLDQNGEGNLDPHAEFSDAETDLHTEEDRGTSFRQLLCIAFDLAVL
                     ISYAKEPFFHFVYHDGGLERLQNKLKLALLRIIRETCATHGIQYIFSALSEDIPPQED
                     SAGLCPAPGEVILTLDDRGDSGRLFKMGTF"
     gene            complement(32444..32881)
                     /locus_tag="PLP58_03480"
     CDS             complement(32444..32881)
                     /locus_tag="PLP58_03480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ07616.1"
                     /translation="MKRSPRKSHLPAALVWLLAGCSVITEKKTLDGFETEQKQSENLI
                     LVTVAEGFERPGSYRLPRGCTLGELVDLAGIKRAQMFINLETTMYALRISSPAKSGTG
                     ETAQSRLVWINYERWNRLIPLSSEDRAILLRDGDQIRRTFISW"
     gene            33705..34703
                     /locus_tag="PLP58_03485"
     CDS             33705..34703
                     /locus_tag="PLP58_03485"
                     /inference="COORDINATES: protein motif:HMM:NF014893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HRJ07617.1"
                     /translation="MSQPSILVIGCGSIGERHLRCFLNTGRCRAAVCDTNGRLVAEVA
                     GRYGVPGFESLEAAQEAFPADGWVVCTPAHTHLALALRGLARGVHQLIEKPLATGLEQ
                     VEETRAAIASSGCHVAVAYVFHAMPWLRAMREVILSGELGSPRHATLTAGQHFPTFRP
                     AYREIYYARHESGGGAIQDALTHSVNALEWLIGPATRVYCDAAHQVLEGVGVEDTVNV
                     CARHGSVLASFGYNQFQAPNELEIRVHCERGSVAMQLHEQRWGMMRFGEKEWTWRRHP
                     KPIERDDFFTWQAESFLEGMAGRPCFLATFEEGVQTLKFNLAALESARSGQPVAIS"
     gene            34700..35653
                     /locus_tag="PLP58_03490"
     CDS             34700..35653
                     /locus_tag="PLP58_03490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009958201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HRJ07618.1"
                     /translation="MSAEIRLAMLGMIPGNGHPYSWSAIINGYDPAAMAACPYPAIPD
                     YLGRQPLETVRIPGARVTHLWTDNPADAPLVAKASLIENIVARPEEVIGQVDAVVIAT
                     DDGDDHARRARPFIEAGLPVFIDKPLATNVPDLRQFARWQREGRVLLSTSGMRYAPET
                     RLSAEQKEQIGDPRWITSFTCKTWERYGIHALEAVAPLVGPGFLTVRAHSQPGSDVAH
                     LTHRSGVQVTLAAIHDAYGSFGAVHLYGTKGQIPLLLRDTYHAFRGQLTAFIEMLRTG
                     QPPLPFSETVELMAVLIAGIQSREKGGGVVEVAEVLKSLEG"
     gene            35734..36513
                     /locus_tag="PLP58_03495"
     CDS             35734..36513
                     /locus_tag="PLP58_03495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009958203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HRJ07619.1"
                     /translation="MTSLDRFSLKDKVILLTGGAGLYGRGLAADLASAGARLVIAARD
                     KAKLEAVAAEERERGFQVEAECLDQGDEASVLALRDRVLARHGRVDGLVNNAVARVVK
                     GAAGSVEQWQESMRVNATGVMLMHRVFGADMVERGIGSIVNIGSIQGMIGPSYELYAG
                     TSMGDLPPDYFFHKGGMINLTRFYAAHFGARGVRVNCLSPGGFFNQQPEPFLSRYCGH
                     TLLGRMADPSDLGGAVIFLLSDAARYITGVNLPVDGGYTAK"
     gene            complement(36557..36898)
                     /locus_tag="PLP58_03500"
     CDS             complement(36557..36898)
                     /locus_tag="PLP58_03500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ07620.1"
                     /translation="MKKILLILTAAAALGAAAPTAAEAWDGRHSHSRVVSYLPCGRPV
                     YAVYQVYGRDRWGQPVGRWVTQHSACGCRACRPPVVVHRPVCPPPVVYHPPVCAPVPS
                     RSVSWFFSFGR"
     gene            complement(36987..39248)
                     /locus_tag="PLP58_03505"
     CDS             complement(36987..39248)
                     /locus_tag="PLP58_03505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ07621.1"
                     /translation="MNLSILLAAVAAHLPGTQPLAAEADFSALMVAGADRFLLRETLA
                     AAARRETFWKRDTTSKEAYSASVAANRERLRGILGVVDERAPFSSLEFVADTASPSLI
                     AENENASVHRVRWPVLEGMHGEGLLIQPKGEVLARVVYLPDADTTPEQLLGFKDGALD
                     PALMNAGAQILIPVLVSRDADFSGSERLGIRINAPHREWIYRQSFILGRHVIGYEIQK
                     VLALVDWFGKQEGGRPVCVAGLGEGGLLALYAGAVDERVGSVYVWGHFAPREGLWQEP
                     VYRNVFGLLREFGDAELATLIAPRHLAVQHAGFPEVKGPPEAGKGVRAVAAPGFIETP
                     PLGSARAEMARALELTNGLAQEWGRVFERKSSAAEIIGHLFPETAARAMLARMAAARD
                     APRVPAQTTSVDAVQARQKRQVREMESFTQRLLATIESERNTGFWKPLPLNSLEDYEK
                     RAAVERGRFWNEVIGRLPDPGVPANPRSRVVRETENVVIHEVVLDVWQDVFAWGWLAL
                     PKDLREGEKRPVIVCQHGLEGLPEHCYETDESTRAFAAYKAFALRLAEEGFITFAPHN
                     LYRGKDAFRSLQRKANPLGMTLYSIINGQHQRIIEWLKARPFTQADKIAFYGLSYGGK
                     SAMRTPSVLTDYCLSICSGDFNEWVRKCATTDLPLSYVFTGEYEIWEWNLGGTYNYAE
                     MSALIAPRPFMVERGHNDGVGIDEWVNYEFAKVRRLYNKLRIGGRAVIEHFDGPHTIH
                     GAGTFDFLKQHLR"
     gene            39390..40607
                     /gene="metK"
                     /locus_tag="PLP58_03510"
     CDS             39390..40607
                     /gene="metK"
                     /locus_tag="PLP58_03510"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017370549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HRJ07622.1"
                     /translation="MSRSYIFSSESVGEGHPDKVADTISDAILDACLAVDPRSRVACE
                     TFVKSNVVVVGGEITIPKLQNKKLGTTKPIDEIINIGKVIREAVRGIGYVNDDDIFHA
                     DKIFINNYLTAQSPDIAQGVDAEKAEGKKTAEQGAGDQGIMFGYACDETPELMPAPIM
                     YAHRLGRELTRIRKAGKQAKWLRPDAKSQVSVEYVDGKPTRIVNVVISTQHAADVSHA
                     EIEKFCIEQVIKKVLPKSMLTKDTEYLINPTGKFVVGGPQGDSGLTGRKIIVDTYGGM
                     GRHGGGAFSGKDPSKVDRSAAYMGRWVAKNIVAAGLASKCEIQFAYAIGHPQPVSVHV
                     DTFGTGAVSDDKILAAVLKVFSFKPADIVKQLNLLRPIYSKSTNYGHFGKDDADLTWE
                     AVNKADALKKAVG"
     gene            complement(40674..41261)
                     /locus_tag="PLP58_03515"
     CDS             complement(40674..41261)
                     /locus_tag="PLP58_03515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007416719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HRJ07623.1"
                     /translation="MRIAIYCGANGGHNPVFKKAAAGLAAFLARQGIGIVYGGGRVGL
                     MGAVADAALAAGGEVIGVIPHSLMEKELGHTGLTALHVTRSMHERKQMMVDLSDGFIA
                     LPGGFGTLDELFETLTWLQLSFHGKPVGLLNAEGFFDGLLAFLDQMRGHGFLKPAHRD
                     CVLVERDPALLLEKMRAFQAPDLGKWIEKMVADER"
BASE COUNT         7961 a        12974 c        13065 g         7466 t
ORIGIN      
        1 ggtcatcatt catcagcacg gctgcaccaa tgcctcgccg gacaagcatc cgcccatcac
       61 aggcgactgg cattggcggg cgctggcgcg gaagcacgac gcggcgctgc tggtgccgat
      121 gtatcacgtc gcgaagagct gcgatgagtg gaacgacccc gacagcggct ccgaaagcgc
      181 tcttttcacc gctttggaag actttgcgca gaggtcgaag cggcctgaac tcaaagacgc
      241 gccttgggtg ctgtggggcc actccggcgg ctcaagctgg tccgcgcaga tgatcacgcg
      301 gcatccgaag cgcgtgctcg cggcctcgtt tcgcggcggc tgccacaagc agtttggcgt
      361 gccggagttt cgcgccaagt tcggccccat cgcccgcgat ctgccgctgc tgtttgtgtg
      421 ggggaaacgc gagtccgtgc ccggctccag tcatttcgtc tcctgggagc cgatgaacac
      481 gatgtatcgc gaactgcgcg ggcagggcgg caaggtctgc cgcgtcattg atccacgctc
      541 cgagcatggc tgcgatgact cgcgcttcat gagcatcaac ttcttcgacg ccgtgctttc
      601 gggcgatgcg aacgccgcgc ctgatgagaa tgcctcagcg ccctggctgc ccaacgacac
      661 cttcgccgcg ctattgcggg agttttccga aattggcaca ctcgtttccg ccaaggctcc
      721 cggccaagcg cccgaactcc aggcacaacg cgaggctgac ggcaaaatca agcttgcctg
      781 gcgcgtcgtt cccgatctcg ccggaggtct gcgggccatc cgcattcatc gcgatggccg
      841 gctttggaaa gatctcgcca ttaaaccaac ggacttcatt gccacaggtc gcgattccac
      901 gcctgaggcg ttgcgcccga tggagcttga ggatgaaagt cgtgacacac acggctacac
      961 gctcacgttt ctcgatgccg ccagccatga gtcaccgcgc agccctccaa ttcatgttcc
     1021 gtgatgtttg cacggccttg ttttccttta ttctggtctt ggcggcgtgc gccgagcctg
     1081 cctggacgtt gtcagacatg cgggccgaca tcgcttataa aagcggctct gatttgagcg
     1141 actacgagcg cgagcgctgc aagctcgacc tctatctgcc tgcggagaaa aaaggctttc
     1201 ccacgctcgt ctggttttac ggcggcgggc tgaccggcgg cagcaagtcg ggcaaggaca
     1261 cgactaaaat tgctcaaagc ttcgcggcgg cgggcatcgc cgtggttgtg cctgattatc
     1321 ggctaagtcc gaaggccgcg tttcccgctt atgtcgagga cgccgctgca gcggtggcct
     1381 gggcgcgtca gcacatcggc gaacatggcg gtgacccgcg caagctcttc atcggcggcc
     1441 attcggcggg cgcatatctc acgctcatgc tcggcatgga cttgcgctat ttgaagaagc
     1501 acggcatcgg ccaggatgcc atcgccggcc tcatcccggt cagcggccag accatgacgc
     1561 actacaccgt gcgcggcgaa cgcggccagg gcggacgcca cgacgtgacc gccgacgatg
     1621 ccgcgccgat tcaccacgcc cgcaaggaca ccgcgcccat gctcctgatt tacgccgaca
     1681 aagacatgcc cgcccgcgct gaggaaaacg aatatttcac ggccgtgctg cgcgccacgg
     1741 gcaacgagca aatcaccgtg cgcaagatcg ccgaccgcgg gcacggcagc gtcggccacc
     1801 gcatcgccga gccggacgat cccgcccgca ccgcgctgct ggagttcatc ggcatgcacg
     1861 gcgaaacgcg gtaaggctcg tgggcatggc ctgcagggat cgttccagtt tttcaaactc
     1921 aataatcctg ccaacccgaa ccaccccaaa ccatgaaccc tcaacactcc acccgtcgga
     1981 acttcgtgcg gcggttcggc ggcacttcct cccagttcgc caacctgccg cctgccctcg
     2041 tcagactttg cgtttgcccc tgcgtgttca cggccttggt cgtgcatgcc gcggaacccg
     2101 tcgagatcgg tcggcgcgtc cagttcttca tggatgatca catcgtggac aaccgctggt
     2161 cgctgaagcc gaagaccgag gaagtggtgc gcgcttttca cgcgccggaa aagcacgccg
     2221 ccaatcccgt gatcgccgga caaggcggct acgtcagcgc ggttcgcgag ccggacaccg
     2281 ggctggtgcg cttgtggtat cagatcgccc actggaccgg cgaggccggc aagggcgcgg
     2341 actatgccat cgcttatgcc gagtccaagg acggcgtcac cttcgagagg cccgagttca
     2401 acctcgtcga atggcagggc acaaaggcca acaacgtcgt ctggcgcggc ccctcgggaa
     2461 ggcgcgccag cggggcgcag atcgtccagg cgccggagcg ggacaagcgc ggtttccgct
     2521 acctcctcac ctatcacacc ggcggcgcgg gcaaaggcat gaacggcatc cgggtggtcg
     2581 gctcccacga tggcatccac tgggacgccg gcagcgactc gctcatcacc gagctgcaca
     2641 gcgacaccat caattccatc gtccacgacg gagatcgcgg ccagtatgtg atgttctgcc
     2701 gcccgaagga ccgctacctt gtgggaggcg ccaccggcaa tttcctcgaa accggcgagt
     2761 cccgccgcat cgcgcgaatg agcggggccg acttatggaa gccgtggacg ggcccgccgg
     2821 agaccatcct cattcccgac gaactcgaca tgcagcgcgg cttcaaccgc ttctacggga
     2881 tgtcggcgcg ctggcacgag ggcatctact ggggagcgct gctctgcttc aaactgaaca
     2941 ccgacatcac caccgaactc gcctggagcc gcgacggacg gcgctttgag cgtttgcccc
     3001 tgcgcccgaa actgctcgcg ctcggccccg acggtgcctg ggacggcggc atggtctatg
     3061 gcagcgccga ctgggtcgaa atgggcgacc gctggtatct gtattacgcc ggtcatgacg
     3121 gcccgcatga gactccccat cgcacgcccg ggatcggact tgccaccctg cgcaaggagg
     3181 gattcgtctc cctgcgcgga cctcgcgggg gcggtgtggt ctgcacgagg ctgctgcgct
     3241 ggcccggcgg tggactgaag gtcaacgccg acgccagcaa aggcgaaata cgggtccgcg
     3301 tgtccgatga aaagcgcagg ccgctggacg gattcggcta cgaggacatg gagaccttca
     3361 ccggcgacag caccgcgctc gaggtcgagt ggcggggaaa atcactcaac gaactccagg
     3421 gccgcgccat tcggctggag tttcacctgc gcaatgccga cctctttaca tttcgcgcgg
     3481 aatagctccg cgcattctcg gtgcgatgcg gagcttccct ttgagatgac gcggaagatc
     3541 atgtgctgcc gcctgagttt ttcgaggagc cctaccatga tgcggcagat gcggacaaac
     3601 gcggtgctca ttctcgtcct cgccaccgta ttcgcctcct ccgccgaatc tccctcccac
     3661 atccccgcct tccccggcgc cgagggtgct ggaaagtggg caaagggcgg acgcggcggg
     3721 cgggtgctgc gggtgacgaa cctggacgac gcgggaccgg gcagcctgcg cgctgcggtg
     3781 gaggcgaagg ggccgcgtac ggtcgtcttt gatgtgtcgg gcaccatcgc gctgaagtcc
     3841 gcgctgagca tcaagcagcc cttcatcacc atcgccgggc agacggctcc gggcgatggc
     3901 atctgcctga aagggcacgg gctgtatgtc agcaacacgc acgatgtcat catccgccat
     3961 ctgcgctgcc ggcctggcga ccaaacctcg aagcccggcg aggtggacgc catcagcatg
     4021 acaaacgtgc gcgatgtcat cgtggatcat tgctccgcca cctggagcac ggacgaatgc
     4081 ctctccatca cgcgcgactc cgaccgcgtc actgtgcaga actgcctcat cgccgaggcg
     4141 ctcaccagcc attccctcgg ctcgatcatc ggctcctatg aaggctccat ctcctttttg
     4201 cacaacctct ttgccagcaa tcgcgggcgc aatccgcggc tgagcggcta ccaggcggag
     4261 gccggacgcg gaggcacctc cggtccgcgc gtggacttcc ggcacaatgt catcttcaac
     4321 tggtactccg gccccggcta caccggcacc gccaagcccg atgagccgga gcgcgtggcc
     4381 ttcaatctcg tgggcaatca ttacaagcct gggcctgaaa cgctggcctc gcagcgcgct
     4441 ctcattttca cccttcatga aggggcaacg gtggaagcct atctggcggg caatgtgaac
     4501 gaggccgcgt cctcagccgc gcaggcggac ctgctgcgga tcaagcccgg cgtgaacctc
     4561 acgctgcgcg agacacccgt gcctcacgag cccttggaga ggactctgac cgccgccgag
     4621 gcttatgcat cggtgcttca aaaagccggt gccacgctgc cccgccgcga tgccgtggac
     4681 acgcgcatca ttgccggagt caaagacggc agcggcaggc agatcgccac gatcccggcc
     4741 gatgcctggc ccgaactgcg ccgcgccgct ccttggactg accgcgatgg cgacggcctg
     4801 ccagacgcct gggagaaagc gcacggcctt gatccagacg acggcacgga tgccgcacac
     4861 gccgcaagct ctgaaggctg gactcatctc gaactctggc tgaacagtcc gcatccctga
     4921 ccaaccatcc atgcgcaccc tttccaccct aacccgccgc caactcatga aaactgcggg
     4981 cgcttgtgtc atgccgttgc ttgtcccgcg tgccttgcgc gcgcaaaaca cggctgaagt
     5041 tgtgcggcat ctgccgcttt ccatcagcaa cgcgccggaa ctgccgctgc cagagggact
     5101 ggtcacgggc tttggccgtt ttggatggag gagcttcggc gtgggcggtg agccggtggt
     5161 attgaaagac cacgcgggtg aaacgagcat ccccgatggc gcggactgcc gcctgcggct
     5221 cacgaacgcc gtggatgtgc gcgatgatca cgcctttgag gccgccacgc cggacgggcg
     5281 gagcctcggc accctcatcg tgcgctatgc ggcgttgttt caggtgcagg agatgcggct
     5341 caccgtggag caggcgcggg cggttttgag acaaggcatt gtcttgaaga tggtgaaagg
     5401 cacgcgcccg gtgtggttct ttgcgccatc gccggaggcc aatccatcgc ctgatgcctt
     5461 gcagctcccg catgtgctgg tggcgacggc ggccgacgcg gcggcgcagt ttgagcagcg
     5521 gatgcgcggg cttgtcatgc tgcaaggctg cggctggcag agcggctgcg tgtcggaggg
     5581 cttgctcgat ctcgcggaga agcggaagga cgcggcactc ttcgaggcgg tggatcgcta
     5641 cctcggcatg tatttcaccg gtgatggcgt cgagttccaa agtccgcgca gcgcgcgggt
     5701 caaagacaag gtcggcggca ttgaggagcc gctgccctgg gccacgctcg cacggcgcag
     5761 accggatcat ccggcgctgg agatcgggcg gaagtttctg agttcgtccg gcatctcgaa
     5821 agacctcgaa tcccccgcca aggaactcac caccgaaggc tgctacaccg tcgcgtatcc
     5881 gatggccgag ctcgcccgcg tcttgaagcg cccggaactc gcggagctgg cgctgcgtca
     5941 ccttgaagca cgcaaaaagg cgaatgtgca tgacggagac atttatcaaa gagccgccgt
     6001 ggaaggctcc aaacgcctgc gcaactgggc gcgtggcgcc tgctggtatt acaacggcat
     6061 cgtgcgcaca ctccaggcgc tggatgatcc cgccgccgcg aaggcgtggc agcccgagat
     6121 ccggcgagtg gccgatttgc tgatgaaaca ccagcgcgag gacggcttgt ggggcaattt
     6181 tctgcacgat cccgcctccg ccgccgacac ctccggctca gcagggctcg ccgccgcgct
     6241 ggcccgcgcc cacgcttccg gctggctcga tgacaaagcg ggacaggccg cgcagcgcgc
     6301 gctcaaaggc ctcgcgaaac acctcacgcc agacggcctg ctcgccggag ccgcgcagag
     6361 caacaaaggc ggcgacgcgc tccagaagag cgattaccgc acgatttatc agatgggcat
     6421 gggcctgaag atgcagctca tggcggcgct gtaattccgg catcggtggt caatgcggaa
     6481 gatcagatga attctgtgtg tctgggcgtt gatgaagtcc ggtgccgctg aaaaaaggct
     6541 ccgcccgacc accgcacaat ttcgcgcctc atgaaatcgc atctgctcgc cctcagcatc
     6601 tctttcctcg ccagcctggc ccgcccgaaa cgatccaggc cgacctctgt gctatgaagc
     6661 cacgcccggc ggcatcgcga tggcggtgcg gtcggcacgt ggggggctga acgtcgttct
     6721 ggtgaatcac aatgatcatc tcgaacatgc aatcccacat gacccagcga ctcggcaaac
     6781 aaggcagagg caccacaatt cccgggttta ttttttgggc tctcatgctc ttttcgatca
     6841 acttgctgac ccagcaactt cgcgccgccc cgcttttcga aacgcaatgc ctctttgccc
     6901 tcaccccgga caacaggccg aactgccgca tcccatccat catcgtcgcc ccaaatggtg
     6961 atctgctggt catctgcgag cggcggaatg acgggccggg agacatcggc aaccatgaca
     7021 tcgtgatgaa gcgcagcggg gacaaaggac ggacgtggag cgaggagatc ctgatcctgg
     7081 atgatgaaga ccgcgtctgc acggacatca ccgcgggcct ggaccgggac acaaagacgc
     7141 tgtggctgtt tttcctgaag gacaaaaaag cagtttgccc atctgaccag cacggacaac
     7201 ggcctgacgt gggccgggcc ggtggtgatt catgagcagg tcgtcaagcc ggagtgggag
     7261 acgctcaggg gcaggccgga caccaagccc cacaacccga agagccgcgc ccagcgctgg
     7321 gagaaggact ggcaccagcg ctacggatgc ggcccgggca atgcgatggt gcagctcaag
     7381 tcgggccgtc tgctggtgcc cgcgcggcat cgcgaggaca tcgggcaggg ccgcctgcgc
     7441 agctttgccc acagctttta cagcgatgac catggcgcga cgtggaaact gggcggcaac
     7501 atcggcctgc acaccagcga gtgccagttc atcgagctca acgatggcag cgtgcgcgtg
     7561 atcagccgca atgaaagcgc cgaggatgcc ccggacaatc tccgccatct caccagcctc
     7621 tcccaagacg gcggcgtgac gtggagcgcg ccgcagcgtc tggagcagct catcactccg
     7681 cgctgccacg gcgcggtgga gcgccatgac gcgaagactc tgctcttttc cgcgcccgcg
     7741 tctcccatcc gccaggcgga gcatccctaa ggacgctaca acctcaccat gcgcatcagc
     7801 cgggacgaag gcgccacctg gagcgccggg cgcacgctct ggccgcatcc tggctcgtat
     7861 tccgacatcg ccgtgctgga tgacggcagc atcgcggtgg tctatgagcg cggcggcaaa
     7921 ggcaccacgc actactggga cgagctgcac ttcgcgcgtt ttaatctgga atggcttgag
     7981 cagccgtgac gttcccctcc catccatcat gagacatctc ccgcatttcc acgcgccgtt
     8041 gggcgcggtc ctcctggctg ccatttccgt catgtccgcg gcatccgctg ctgatgcggg
     8101 gtccgcgtgg gaaacaccgc ctggatggac ggagacacgc ggcggcgagg gcggaaaggt
     8161 tttgcgcgtc accacgctgg ccgccagcgg cgcgggctcc ttcgcggagg ctctgctggc
     8221 gaaggggccg cgcatcatcg agttcgacgt ggcgggtgtc attgacctcg gcgggcgttc
     8281 tttgaagatc tcaaacccgc aggtcacgat ttcgggcgag accgcgccca gcccaggcat
     8341 cacgctcatt catggcggcc tgagcgtgag caccgatgac gtgatcatcc ggcacctccg
     8401 catccgtccg ggcgcgggca agagggcgaa gaagagcggc tgggaggtgg acagtctggc
     8461 gacgaattcc gcccatgatg tcatcgtgga ccattgctcg gtcacctggg gcaccgatga
     8521 aaacctcagc gcctcgggac cgcgctttga gggcgcgacg ccggacgagt ggcggaaaaa
     8581 cacctcccgc cgcatcacct tcagccactg catcatcggc gaggggctgc atgactccac
     8641 gcacagcaaa ggcccgcatt caaaaggctc gctcattcac gacaacacct ccgacatcgc
     8701 catcctgggc aatctttaca tcagcaacga tgaccgcaat ccgctgttca agggcggcgc
     8761 gcgcggcgtg gtggtgaaca atgtgattca gaaccccgga agccgcgtca tgcagtgctc
     8821 gctcgttgag agcgagtgga aaggcaagga gtggcagcgg ggccagatgg ccgtcatcgg
     8881 caacgtcgcc agacaaggcc cgagcacgcg cgcttcggtg ccattcatct cctccaaagg
     8941 cccatgggat ctgcatctgc acgacaacac ctacacctac gccagcggcg aggaattatc
     9001 agtgaaagcc agcggcacga acaaggagct gcgcatcgtg agcgagccgc cgctctggcc
     9061 tgcgcgcctg aaggccaggc cagccagtga aaccgcggaa tgggtgctcg ccaccgcagg
     9121 cgcgcgcccc tgggaccgcg atgaaaccga ccgccgactc atcgaggagg cgcgcacagg
     9181 agccggaaag atcatccatc atgagtccga agccggagca tctggcgcga gcaggcgctg
     9241 aaaaatggac gcgatcttcg ctgggccgga ttatgtcacc ccatgaagaa ggagttatcc
     9301 aaaaagcagc gggatgagtt ggccgaggtt ttgcgcgcgc gcttcggcaa gaatccccat
     9361 cgtcatcagg gcatcgaatg ggcgcaggtc ctggcaaggc tggagacgca gcagggcaag
     9421 ctgtggtcgc tgggcgagat ggagaaaacc ggcggcgaac ccgacgtggt cgggcacgat
     9481 cccgaatcgg gagaattcca cttctgcgat tgcgcccctg aaacgcccaa gggccggcgc
     9541 agcctgtgct acgaccgcgc ggcgctggac gcgcgcaagg agcacaaacc ggcgggcagc
     9601 gccctggacc tcgccagcgc gatgggcgtg gagatgctgt ccgaggagca gtatcgcgaa
     9661 ctccagaagc tcggcccttt cgacacgaag acttcaagct ggctgctcac tccgcccgag
     9721 atgagaaaac tcggcggcgc cctctttggc gactcacgct acggccgcgt cttcttctac
     9781 cacaacggcg ccgaatccta ctacagctcc cgcggcttcc gcggtttgct gcgggtgtga
     9841 gcaaagccag ccgcagccaa ggaacccggc acggcgcctg ccaaagcaga tggaggggct
     9901 ttcatcacat cacggcgtct gaaacaggca tgatgtgatg ctggcaatat catgatgcgc
     9961 agagcatgcc atcgtccctg tgatcacaag gcgcggcttc atgctggtca cgctcattga
    10021 ccagaaacaa agggctcagg atttcgtctt gatcatggca atgagacttc catttttgat
    10081 cctggccctg agcgtttccc tggcgcaagc ggagctgcgt ctgcccgcgg tcatctccga
    10141 tcacatggtg cttcaggcgg gcaagcccgc ggccatctgg ggctgggcgg acgcgggaga
    10201 cgcgatcacg gtcgaattcg cggggcaaac gaaaaccgcc gccgctggag tggacggcag
    10261 gtggagcttg cggcttgagc cgctcactgc gagcgcgcag ccgcgcgcgc tgcgtgtcaa
    10321 tgacagggtc attcgggatg tgctcgtggg cgaggtgtgg cttgcctccg gccagtcgaa
    10381 catggccatg caaatcaacg gccaactgca tggcaaggtg gacaacgccg atgccgaagt
    10441 cgcggcggcg aagttccctg aaatccgcgt cttcgtgcat gacacgcctt ttgccattta
    10501 cgaactgcca gtgcctgctg acgagcacgc tcaagatcgt ccgggacgct ggcgcgtgtg
    10561 ctcgccagag accgtggcgg atttttcggc gatgggctac ttcgtggcgc gtgacctgca
    10621 cagggagttg aagctgccca tcggcatcgt gacagcggca gtgggtggca cgccgattga
    10681 ggcctggacg agccgcgagg cgcaggaagt cgtgccggag ctcaaaccat tgctggcgga
    10741 ctggacaaag cgcctcgacg gctttgtgcc ggcggtggcg caggcaaagt ttcttgacgc
    10801 gaaagcggcg tggctgaagg agcgcgcgaa ggcgctgaag gccgggcagc ccgcgccgaa
    10861 agcccccgcc cccttcaaaa acatccaggt catgaagccg ggcggactct tcgccggcat
    10921 gatcgcgccg ctggcacctt acacgatgcg cggcgtgctc tggtatcagg gcgagcgcaa
    10981 cgcggcgggt ccgttcaccg gtttgtatgg caggcagctc gaaaccatga tcgccgattg
    11041 gcgcgcgcgc tggcaggacg agctgcactt cgcgtgggtg cagcttcccg cttttggcgg
    11101 cgcgcagaag ctgccctccg aaccgaacgg ctggggagtg gcggtgcgcg agggccagcg
    11161 ccgcgccttg cgcgtgcccc gcacgagcat ggcgatcaca atggatctcg gcggtgagaa
    11221 atccgggcac ccggccaaca aagctgacta cgcccgccgt ctctccaccg tgctgctgca
    11281 cgacgtgtat caaaaagaca tcgctttgcc caccggcccg ctgttcaaag ccgcgactgt
    11341 cgctggtgga cggatgattt tctccttcga tcatgcggaa ggcttgaagg caagagacgg
    11401 caagcttgag ggcttcgcca tcgctggagc cgatggcaag ttccactggg cgaatgcgcg
    11461 gattgagaat cgcgaagtca tcgtctggca cggcaaagtc cccgatccgc aaaccgtcca
    11521 ctatgccttc gccgccaacc ccaaaggcaa cctcgtcaac gccgccggcc tgcctgcctc
    11581 gccctttgcg gcaagcgccc cgccagccgg gcaggccggg cagtgagcgc gcggatcatt
    11641 gctccaggcc atgccgaagg cccaggtccg ggcaaaggcg gtgtcagcgc accagttcca
    11701 catccgggcg cttctttttg aaggcggcga cgccggcgtc ggtgacgctc gtcttgctga
    11761 tgtagagctt tctcaagggc ttcagacctt gcagttgctc cagtccctgg tcgccgagcg
    11821 tcgtgttatc cagtttgagt tcccgcagtt gtttcacagc caggagagcg gtgagctggg
    11881 cgtcggtgac ctggcactgg gtaaggtgga gttcctccag cgcggtgaac gcggcgagag
    11941 cggcgagggc ctgatcctca atgcgggaga aggtggggaa attgaggttg ttgatcctgg
    12001 gccatgcctc atggagggcg cgcaactggc tttcagtgac cggaaccttg tcaccgcccg
    12061 gcgggtagtt gaattggagg atgcctggaa agaccagctt caattccggc aaggctgtat
    12121 cgaggaccga tggattcgga ccgaatccca gctccttcag aggcagtttt gccagcggac
    12181 ggaccccggc gacggtcacg ggagtttcgg tcacgtccag gaagtcgagt tttttgagcg
    12241 cggccagctt ggcggtgccc tcatcggtga tcctggtcct gcgtaaactg agcctgctga
    12301 gtttgggcca ggtggcaaag acggccagcg cggcatcgtc aaccgcactg tcacgaaagg
    12361 tgacgcgctc catctccgcc ccctgaatct gcgtgaccgc agaagcgtcc ttttgcgagc
    12421 tcgcgatgct ggtgatgctg acgagatttt tcaggctcag gagatgttga agccaggcgc
    12481 ccttcatttg cggcccgccg ttctcgacat gcaccacccg caactcggga caggtggcca
    12541 ggaaggccag ggcttcatcc gtgttgacgg cccggttggt gtaaaagctc tggaggctct
    12601 gcatgcccgc aaaggcggcc gtggcggttg cgtcaagcgc ggttggaagg gaggcgaatc
    12661 gcagttcgag aacggacaaa cggcccttgg gcagaccggc ggcgtcattg atcaagggac
    12721 tttcccaagt catcctgacc tggccgccca ggcggagggc ggtttccgcc gcggaacgat
    12781 catcactttt cgcctggctg cctgtggttg caggaggcgc gggggcggca gggccgggag
    12841 tggcgtcaag gccgggagtg gcgtcagggc cgggagtggc gtcagggtag ggggctgcgc
    12901 tgctgcggag gctttcgcga taggagcgga cctctttggc ttcgtcaatg cgtccggctt
    12961 tggtcagggt ggtctccagg tcctgcaatc gggactggta ggcgggcagt acgccggcca
    13021 gcgcggccgc gcgggtgtct tcgagcttct tcaactgccc gcgataaatg gcgcggaggc
    13081 tcttgagcgc gtccggtgtt tgttccccgt catcagggat gggaagtttt tctgcgaggc
    13141 gctttttatc gtcctggatg gcgagcacgt catcaagctt cccggtctgc tgcgcggcgg
    13201 tggcggcgct gtcgagcgcg gacgaaaatt tcgtgttcag cgcctccagc gcggcgtcga
    13261 agggagcggt cacacgttcg ccgaggacct tgtcatactg tccgcgcagg gcggtgagtt
    13321 cggccggcgg tgcggcgtga atgaccagtg atgaaagcgg aatgacgaag gcgagaaggg
    13381 cgggagtggg cttcatggat cgaatttaaa gccatatcat tagaagacgc atccgcgcaa
    13441 gcaagacgca ggggtccggc tcctttcagc cctgcggtgc caaacccgag ggcaagcctg
    13501 tgaatcacgg ctccagtaac atgcggaaaa agcggcgtgg tgatgtgtcg gggttgaggt
    13561 cgaggtgcag gcggcgcgtg ttggcggggt cggacggaag ggacggttcg ggctgccagt
    13621 cctccggcag ttcacccagc ggctgccaca tttcggagcc aagatgggtc acatactgca
    13681 tcggtgtgta ctccatgccg acggcgtggc tgcgcagggt gtgaaggcgt atgccgccat
    13741 cggggcgtcg actgatgcca aggatggcgg gaggaaacat gtcgggcgtg tgcgggttgc
    13801 cggcgagcag atattcgatc cagtcgctga cgaggtcgcc gtcggagtct gcgtccgggg
    13861 cggcgttcgg agcgagctgc atgtagtccc gccactgggc gtagttcatc ggcgcgtgcg
    13921 gattgccgag gtggagacgg cgctcgatga agccggtgat gtcctggaag gacgatggtt
    13981 ccggcagggg cggcgcgggc aaggtggtga ggggctgaag cgtgtagcgg gaggtgaagt
    14041 cctcggtttc ctcgtagccc tgcggcggcg aggggacgtc gatcgtccag ttgccgccga
    14101 gcagatcata gacgctgggc tggaggtatt tcacgagggc atgggtggaa ggatgatagg
    14161 gcaccgtgcc ttcgtagggc tgcttggcga gggtcaggat gggcagcccg ccggagttgc
    14221 cgaacgactt tgacagagtg aggtagtaaa tgccctcgtc ctccagaagc atgtccacgg
    14281 tggcgtcggg ggaaccgtct ccctcgtgcg tgaccatcca gcgcacgctg agtttctccg
    14341 tcagcagagc gctgatgggc gcggtgttgt cgccgttgcc ggtgacaccg ccgatggcga
    14401 gtccgttggg agtccacgct agcgtgtgga gcaggtcgtg cggcgtgtag ccggagtctt
    14461 cgagccattc ggtggcgttc gggtagttgc gcacgccctt gtccggcccg tcgatgaagg
    14521 cggtgctgtc cacggtgggt tcgcacaggc tgatcatgcg gtcaatcgtg ccgtcgggca
    14581 gataacgacc gatcgccatg gcgggaatgt ccgcggtgac gatgggggcg aagtttcccg
    14641 cggtgaagat gaccccgtcc gggttctcga cgacacgagt ccaggtgatg gcgggcttgc
    14701 tccgcacgga ccagacgaca tcccaatccg tgggatccac tttcatcagc caacctcccc
    14761 attcggcgcc ggcgttgatc ggagtgccgt cggaattgcc gcgcgcctcg cctgccagca
    14821 gcagtccgcc gtcctggagc aggatggcat cgcgcacgct ctcgagcata ttgtgggagg
    14881 cgaagcgatg gctggcgagc gcgttgccct cgtcgtcgaa acgcatcacg atcgggtcgt
    14941 tgtcgaaaat cgtcgcatcg ttgttcacgt cgccaacgag gaggatctcg aagtcgccgg
    15001 gtgtggtcgg ccggatgatg acactggcga tcttggtgag cttcgtggtc tgtcccgggt
    15061 cgtcattgac ggcgactgcg cctttcaccc acagcaccgt gccgttctgg gagaagcgcg
    15121 cgatgacctg cgcactcgcg gtggtctcgc agtaggcgat gaccccgcca tccggcgtgg
    15181 cggcgatttt ccgggggaac aggaagttga cacccttggc ccagagcgga ttgccgcgct
    15241 tgtcgagact cacgaccggc ggcgcgacct cgaaggcgtt gggtgcgatg aggaaggtct
    15301 cgccgttgac gccgggcagc ttcgagcgga cgagttgcgc ggtgtagggg gcggggccgt
    15361 cgttgttcca ttgcagcgtc ttggcccagc gcacgtcgcc gccctcggcc ggatcgagcg
    15421 ggccgggcgg aggatggccc accgcactgc cgcgcgtggc ggggccatcg gaatcggtgc
    15481 tgaacaactg cgggccgttg aaggtggcga tgaccgggtc ggcgagttcg aggccgagca
    15541 ggcggatgct gaagccgctg aggatgtcca cgtccgcgtc ggcctcccac cagtccgggg
    15601 cgaagggtgt cacgttgaag ttgaaattgg cgcgcgggcc gatgtagggg ccggcgatgt
    15661 cgttgatgat gagcgccgca cgcgcctcgg cccaggcggt gacgttcagc gccagcgagt
    15721 cggcgatctg cggcttgctc aacgtgagcg ggcggtgttc ggagaacggc tccgagctga
    15781 cctgcatgcc cggtggtttc gtgaggtccc agatccactc cacgccggcc tcgtaggagc
    15841 cggtaaaggg cagcaccacc tgcgtcggca cgctggccgt gacgccgacc ttggcggaga
    15901 atcgcggctg caccttcagc gggactccgg cgatagagaa gtagatcgtc ggcagcccga
    15961 aatccacgat ctgcttctcg cgctcgatct cggcaaggcc gttgttgtcg gtgccggggt
    16021 tgacggtgac gcgcaggttc agctcgagtt cgctgccaag gcggacggtc acgcgcaccg
    16081 gcaacaggcc cgagtagcgg acgtctatcc cgactttcat cccctgcaac agcacctggc
    16141 cgtccaccgt gatgccgcgc gtgatcttca gatcgttgat cggcatgtgc agggcgaaga
    16201 aaccgttcag ttccgaaagc ctggggtgtc gcaccgcgtc cccgttgagc aggccgttgc
    16261 gttcggcctc gagctggagg ttttccaggt aggtgaaggc ggtgtttccc gcaggcgggt
    16321 cgaaggggtc gcgggccgcc ggatcgccgt ccgccggcgg atcaaaggcg tcaatcgcct
    16381 ccacgctcag cgtgccttgg cgcaggttgt ccacgaagcc gcgcaggttg gggtccgtct
    16441 gcgagttcca gaatgggaag atcgtgctga acaaccagtc cggcggcgcc cctccgaact
    16501 gctggccgtg cacgcgcacg ctgctatcgc cctcgaaggt gaccgaggtg acctcgacca
    16561 tccggtcggt gcggatcgag tggcttgccc ccatcacata ctgagaggag gacacatgtt
    16621 tcgccagtcc gatcaacttt ggcttctcgc cctccggtgg catgtcaaag gcccgcggca
    16681 aggtatgaag aaacggcacc tccatgagca gcgtgccggg ctgaagaccg gcgagcagcg
    16741 gcgtggactc ggagaagatc agcgtgacac cactcgtgag gaggttttcc gtcgcgctgt
    16801 cataggcctc gcccgtggcc tcatccacgg cgacgggcgg caggcggaac tgaaaaagct
    16861 ccgtggccgt ggcccgcagg cgctggccag ccagcacggc gttcttcaac gccaaagcgt
    16921 ccgccgcagg cgcgagcagc acgatggtct cgggcatcgc gactgtgctt tgaggcgcga
    16981 tgtccgcaaa cacgtgcgtg tcatccacga tctccaccgg ccagcccggc ccgggcggat
    17041 cgaaggcgat gcgcgcgctc tcgtagtagc tgtcgccgga gttttgcagg cttgccgtga
    17101 acgacagccg gatgcgcccg tcgggctcgg tcgtctcccc ggcgagctgg atgttcacga
    17161 tgctgacgtc gttcgcggcc tgcgtcaggc gcgggatctc cgtgccgtaa ccggagatgg
    17221 cattgtaatc cgccggtgcg gccagcaggc cattgaaacc ggtggccagc aggctgaggc
    17281 cgaggagagc gatgcgcagt tgcattcatc cggcagtgaa cgaattcagg gcggatgttg
    17341 cagagaatct caaaaaagtt gccgcccccc ggccgatgtc ggaatcttcg cccgcttgcc
    17401 cggcacctct tccatctccg ctgaccgatt ttttccacca acggcctgga cgctggtgct
    17461 ggaggccgga ggcgctgcgc cagatcatgc gcgcgaggaa ctctgcaaga cttactggaa
    17521 gccggtggcg ctctttcttc agtcgctcgg cttgagcgag ccggacgccg aggacggcgc
    17581 gcaggaaatc atggcccagg ttttcggaag cgagggcttg aagccgttct cacgcgagca
    17641 ggggcggatg cgccactacc tcaagtcggc ggcgcggcat tatttcttca accatcgcag
    17701 ggacgcgaac acacggaagc gcggcggcgg cgcgcccacg ctgtcgctgg atgagatgcc
    17761 gcctgtgccg gtcagcgagt ccgcgtgcga cgcgctcttc gacaaggcgt gggcgctggc
    17821 cctgtgcgaa cgcgcgatga gcgcgctgga ggccagttgc acccgccgcg gcaaggggcc
    17881 gctgtttcac gcgttgaaac ccacgctgac tctcggcgag gaagtgaaac cttatgccga
    17941 aatcggttct atgttcggtg tgaccgagca gcagatccgc atcgaagtct tccgcctgag
    18001 acgccgcctc gccgaattcc tgcgcgcgga ggtggccgcg acgatgcagt ccggccaggg
    18061 cgcggcggaa gtggaggatg aaacccgcca cctcatccgg gcgctcgcct atgaacccgc
    18121 aagctgacac cacctgcgcg ggctgcggtg cgccgctcgg caaggacgtg ctgggcggcg
    18181 tctgcgcggc ctgcttcgcg cgcggcgcgc tgctgcccgg gggaggctgg ctgcaatcgt
    18241 tgccaaccag tgatgaggaa gcgtcccaat ggcccgagct gcccgactgg cagatactcg
    18301 gcgtgctggg cgctggcggc atgggcacgg tctttcgcgc gcaacgccac gccgacggga
    18361 tcatcgccgc gctcaaggtg ctcgacggcc gctggtctcg cgacccgctg atgagcgcgc
    18421 gctttgaagc ggaggcggac gcgctgaaac ggctggagca tccaaacatc gtccgcgtgc
    18481 tggagacctg cgaagcgcac gacggaagac tgtgcatcgt catggagctg gtggacggtt
    18541 gcgacctcgg caggctgatg cgcgccggtc cgctcggcgc ggcccgatcc ctggacatat
    18601 ttcaaaaggt ctgcgccgcc gtcgccttcg cccatgcgcg cggattttcc catcgcgaca
    18661 tcaagccggc gaacatcctc gttacgggcg atgacggggt gaagctggcc gacttcggcc
    18721 tcgccaagga atggacggag ggaaccatcg gcgcgctgac ggcgaccacc gaccagtttg
    18781 gcaccgccta ctatctcgcc ccggagcgca tgatctcgcg ttccccggac ggaccgccgc
    18841 aggacgtgta tgcgctgggc gtgctgttgt atcacctgct tgccgggcgc atgccgatcg
    18901 gcaactacac gccggtctcg cgtctggcgc ccgtgccggc ggcgttcgat gcgctgatcg
    18961 ccaaagccct cgaagccgat cccgcccggc gcatcgaaag cgccgacgca ctcggccagg
    19021 ccatcgccaa agcatggcgc gcgcacgaga acagcggcca gcgaagacaa aacgcacggc
    19081 ggctgctgct ggttgccgct gctggtgtgc tgtgcgcggg cctgttgact ggcggggcgg
    19141 catggcagcg atccagagtg ccggagcggc ccgtcttcac gccgccctcc gcggtcaaag
    19201 ccggttccta ttgggaaaac agcctcggca tgaggttcgt gcccgtgccc ggcacgcgcg
    19261 tgctgttttc aatctgggag acgcgccgca gggatgtgga acccttcatc gcggtggaaa
    19321 aagccaccgt cgccgccagc taccgggagg tcgaggcgga acgaagccgc aagagtttca
    19381 acatccagac gcttggactg ggcggacggc tcatcgccgc cggtagctgg gcggagccgg
    19441 gcttccctgt cacggacgat catcccgcgt gcttcatgct gccgcgtgat gccgcgcgct
    19501 tctgccagtg gctcacctgg aaggaacagg cggagggccg tctgctgccg ggccagtgtt
    19561 atcgcctgcc cacggctgag gaatggctca tcgccgccgg tggcgaggac acgccgccgc
    19621 gcgccggcaa cgtcgccggc cccgaggcgc gggatgaatt ttggaacccg cgctggccca
    19681 cgttcgacaa ggcggatgcg tttccccgca tggcaccggt gggatcgttc ccggcggagt
    19741 tgcacggctt gttcgacatc tcgggaaacg tcagcgagtg ggtgctgcgc tccgaggcgg
    19801 acatcatcaa gccgtcctgg gaaaccgacg ccctgctgct cggtccggac tttcacgacg
    19861 gcagcgccga cgtggccagc ttccgccacc gccgcccttc gccgatgaac ctgcgtctgc
    19921 cgaatttcgg cttccgcatc gtcctggagc atgagatcac taaggcgcct taagcgttgt
    19981 ccgcgcgggt gcattccatt ggtcaaatca caagaccaga taatccggct cgcagggcgc
    20041 gtcttgaaca gcgtcttctc attgtcatgc ctgcccacgc cgattcgcaa accagccagc
    20101 ccgtgagaac tcctgctgga aatgccagac tcgaccgcgc ctttgcgccg ttcgacatcg
    20161 cggtttgccg ggctgcccat gcgggaagca gagtggcgtt actgaaatcc ctccctgtgt
    20221 tcctctacgt cctcggtggt tgtctcaaag ccgccgactt ccgcgtcgaa aacagcaccg
    20281 tgctcacgag tgagggcgcg tatcactggg cgcagtcgcg gcctgcggtg attcctccgg
    20341 gcaaagtgat cgtgacgaca caggagatcg aaaagcgcgg ctcgcacggc taccgcgaca
    20401 tttacatcac tgagacgacc gacggcgcaa agacctggag cgcgccgaag cgcatcgccg
    20461 cgctcaatcg caagcgcttc cctgacggca tcgaacgtgt gatgggggac atctgcccgc
    20521 agtggcacga gaagtccggc aaactgctcg tcaccggcaa gtgtttcggt ttcctcgcca
    20581 atgcgaacga caacaaagcg aaggacgacc gcagccagga gcgcgtggct tacgcggtgt
    20641 atgatccgaa gacgaacgag tggagcggca tgaagatcat ggacatgcca gggaaggacc
    20701 atgctgggaa tccggtcatc gaaccgaacg cgggctgcca tcaacgggtt gacctgccga
    20761 atggcgatgt gctgctgccc gtgcgttatc gtgccgatcc gaagacgcgc gtttacacaa
    20821 cgttggtggc gcgctgcacc ttcgatggcg agaccctgaa atatgcggag cacggcagcg
    20881 agcacaccat cagcatccca cgcggcctgt atgagccttc cgtgtgcgct ttcaaaggcc
    20941 gccacttcct caccatgcgc ggggagaaga acggccacgt cacgcgcagc gatgacggat
    21001 tgaattacca gccgacagtc gagtggaagt ttgatgacgg ccagccgctg ctcagcgcga
    21061 acgcgcagca gcattggatc acgcacggcg acaagcttta cctcacctac agccgcaaag
    21121 gcgcgaacaa cgatcacgtc ttccgcaatc gcgcgcccat cttcatcgcc gaggtcgagc
    21181 ctgagaagct gtgcgtcatt cgtgccaccg aacagatcct gatgcctgag acgggcgtcg
    21241 atctcactgg cggctttgcg cctgtggatg tgaatgcgaa tgaaacctgg gtcatcagca
    21301 gcgaaatggc cttccccgaa gcccgcaagg acgaaaacaa ccgtgtgctg ctcgccaaaa
    21361 tccactggaa ccagccatga aaaccatcct gcccatcccc gtcgcgctct actcggcctg
    21421 catcaccgtc attcgtatct tccccgtttc actcgcgctg ttggtgtgca aggtctcgct
    21481 gtttccagcg gacttgttgg cgcagccact gcaaaccgac ctctgcatct acggcggcac
    21541 gcctggcggc atcgcgatgg cggtgcgtgc ggcgcgggag ggcttgaatg tcattttggt
    21601 gaaccacaat gaccatctcg gcggcattct ctcgaacggg ctgggcgtgt gggacacgct
    21661 ttgggagggc aagcgctcgc cgatttacga tgaggcgcgg ctggcgatct ttgaccacta
    21721 ccgcacgacg tatggcgagg actcgcggca gtatcgtgat gcgctgccgg gcaagagcgg
    21781 gcacacgaac gggaagtttg agccgaaggt ggcggagaaa attctcacgg aactcgtggc
    21841 gagggagaag cgcattcgtg tgatcacgca tcgcgtgccg tcggcggtga agagggatgg
    21901 ggctttgatt caatcggttt ctttcatgag tttgtgggcc aatgtggagc ctgacgaggt
    21961 gcaggcgaag attttcgcgg attgcagcta tgagggcgat ctcgcgacgg tggcgaaggt
    22021 gccgtatcgc atcgggcggg aggctcgcag tgagtataat gaacctcacg cgggtgtaat
    22081 ctacatgtcg ccggtcaaag cggcggaggc acctgaactg gcgcgagcgg cggaactaca
    22141 tcaccagttg aagctgcgga agttcagcgg atttcagcgc atcaagaagc cggagagctc
    22201 gggcgaggcg gatggctttg tgcaggcttt caactaccgt acgatcctca gctccgatcc
    22261 ggcgaaccgg cttccggtcg aaaagccggc caactacgat cctgagaagc tcaagctgct
    22321 ggagcacggc tccatcgtgt cgccgatccc gaactcgaag cgcggctgga accgtcccca
    22381 gctcgtcgga ttgcaaacgg gctatgtcga ggccgattgg aacggacggc aaaagatcat
    22441 ggacgcgcac tgggacgcca cgatggcgct gctctacttt ttgcagaacg atccgtcggt
    22501 ggagccggag cggcagaaat cgtggcgtga gtttgggctg gcgaaggacg agttcaccga
    22561 caacgggcat cgtccgcatg agttttatgt gcgcgaggcg cggcgcatca cggggcgcta
    22621 catcttcacg cagcatgatg cgatgctggc ggatggcctc gaacgcgcgc cggttcatga
    22681 ggacagcatt ggcgtgacgg agtggtatct cgacacgcat gcctgcacgc cgcgccgcat
    22741 tgaaggagcc ttggaggaag ggaaaatgat gctcgatgtg gagacctttc ccggtcaggt
    22801 gccatatctg gcgattttgc ctcagggcgt ggacaatctg ctcgtgccgg tgtgcctgag
    22861 cagcacgcat gtcgcctggg gcacgatccg gctggagccg acgtggatga acctctgcga
    22921 atccgccggt tatgctgcgg cgcttgcggt tcaaaacaag attacgcccg cagcactcga
    22981 ttccgatctg ctgctgcgca aactagccaa cagccatgtc atgctgagtt ttttcaacga
    23041 cgtggatgtt tcctccgacg atccgcgagt ggccgcggcg cagtatttcg ccacaaaggg
    23101 cttctttgcc gattacaacg cgcgtctcga cgaaccgctc accgagagcg tccgtgcgac
    23161 atggggcaag gggctcactg cgatgcgtga aggcacgctg aagccgatgg aactcatgaa
    23221 acaggtgcat gaggccgagt tggcaagttc gaagcgcatg gagcgcacgc gaggcgaggc
    23281 attgctgggc atgtggaagg tattgccagc aaagaaggcc aagtccacgg cgcgggtgcc
    23341 gctgaaaatc gtgccggtgc gctccagcac ggcagcggag gtgaaagaca agtcgtttga
    23401 ccttgtggtc gtcggtggca cgccgggcgg catcgcgtgc gcggtgcgtg cggctcgtga
    23461 gggactgagc gtgctgctgg tgcagcacaa taagcacatc ggcggcatgc tgacgaacgg
    23521 gctgatgcag tgggatgctc tctatggcgg gccgcgctcg ccggtgttca atgaatgcgc
    23581 gaagctgatc gagaatcact accgcgagac gtatggcgag ggctccccgc agcactcgca
    23641 ggctcgatac acgcagtcgc attatccgat gagccgtttt gagccttcgg tggccgagca
    23701 tttgttcaac aagctcgttt cggcggagaa gaacatcacc acgctgctgt cgcattatcc
    23761 ggtgcaggtg aagcgggagg gcgcgttgct caaagcgatg actttgcgcg aatacggcac
    23821 gacgaaggac atcaccgtga ctggcggcac ttatgttgat gccacttatg agggcgacct
    23881 ggccgcgctg gcgaaggtgc cgtatcgcgt gggacgcgag agtcgcggag aatacggtga
    23941 gcctcatgcg ggcaaggttt tcaccaacat cgagtcgaag aaggggccaa tagacgtgtt
    24001 ggaaggccgg ctgaaccttc acacctatgc tcatgtgcag ggcagcattg atccgacgag
    24061 cccgttcacg gcggatgggg cgatccaggc ctacaaccac cgtttctgcc tcagcaatga
    24121 agcgaggaac gtccggctgc cgacccaacc gcccggttac aaccgcgagg agtatgtgaa
    24181 ctacaatcgc aagggcatgg gcgcgggcgc gctgaacggc aagagcagct tcaacagcgc
    24241 catcctgccc ggtgagaatc acgcatatcc cgaggcgacc tggccggagc gcgagaaaat
    24301 catcgcacgg cacacgaact tcgcgttggg gttgatgtat ttcctccaaa acgacgaatc
    24361 ggtgccagag gcgaagcgcg caggctttcg caagatcggt ctgccgctgg atgaatacac
    24421 cgacaaccac aacctgccct acgagctgta tgtccgcgag gcgaggcgca tcgtgggccg
    24481 atacgtcttc acggaacatg acaacatggc ctccagaggc atgacaagaa cgccaattca
    24541 cgccgacagt atcgccttca ccgactggtc gatggactcg catgattgca cgacggatcg
    24601 caggccgggt tatgattacg acggcaaact gatcctcacc gaggagtcac gtcctgcgca
    24661 gattccgtat cggtgcctgc tgccaaagga gatcgacaac ctgctcgtgc ccgtgtgcct
    24721 gagcgccacg catgtcgcct ggggagcggt gaggcttgag ccggtgtgga tgcaaacggg
    24781 tgaagcagcc ggttttgctg ccgctttggc gaaaaagcat caatccacgc ccggaaagct
    24841 cgatcctgat ctgctggtgc gcacgctcgt gatgaaccgg catttcgtga ccttcttcaa
    24901 cgagttgcaa cagcacgcgg atcatgccgc gatgcctgcg gcgcagtatt tcggcacgaa
    24961 agggttcttt tccgactacg atgcgcgtct tgatgaaccg ctgaccgaag gtgtccgcgc
    25021 ggcatggagc caaagactca aggcggtgct tgacgccggg atcacaatct cgaaactcag
    25081 ggaacaaaca cgcggcgagg cgctgctgga cttgtggaaa tcgttagcgg actagcgggc
    25141 tgccttgctg aagcttgacc gaaaagcggc ggaggtcgcg ggccaggact ggtcgcgttg
    25201 atgcagttct ccgggatagg cggcgtccga gttccagtag gtgtgggcgc ggatgccata
    25261 agcgcggatg aacgcggcca tgctttccac aaagggcgcg gcatcaggcc cccacacgcc
    25321 ccattcatca atctggaagg attttccccg tgctttggca aaagccagca tgcccgttag
    25381 attccaggca ttgtagccgt gttcgctggt gcgtttctcc caggcctcgc gcgggtcgtt
    25441 ggtctcccag ccctcaccac ccatggcctt gggcttccag taaacatcgt agcccacgag
    25501 gtccacatag gcgtctccag gccacatcgg gcggggatcg ctcgcgccct ccgaggcgga
    25561 aaagctgaag cggaagcgtt ccccgcccgg cactccgcga aagactccat gaaaacgccg
    25621 ccaggacgcg atgaaatccg gcacctgcgc gaggcgcgag aggccgacac cccagacgaa
    25681 ccaatcgcca ttcatctcat ggctgggacg cacgacgacg tcatgcgtgc cgggattgtt
    25741 ttggaggatg gatttggcaa ggcaggccca gtgctcgtca tacgcgccct tctgcgcctc
    25801 cgcatagctg gcgccttgga taatgaccgg cacatcgtaa acgagacgcc tctgaccgcg
    25861 tgctccatac accgtcttgc tcagccagtc ggcgtagcgg tagttctccc acttctggaa
    25921 atccaccgtg cacaggatca catccactgg acgcccaagc cacgcctcga agtccagcac
    25981 acgctgcatg gagttttcac cataaacacc caccagcggc tccgcggcgg tggcgcacat
    26041 gggggaaagg ccgatcagcg cagccgcagc cagccatttt agagacagag aaacaaacgt
    26101 catggcgaaa gctgtaaccg gaagcagcag ggcgcgtcaa caagcaggag gtaacctgtc
    26161 aataatgggc gggcatttcc aattccatgc cggtgcagaa agggccttgg aatgctcatt
    26221 tcacgataaa attgtcgtca tcgagcctga gacgacaaaa ggattctcgc tcaagatcgg
    26281 aaccttgccc ggttgtcatg cggcggccga gcttgaaaag ctcattcctc caacggtggc
    26341 ggcagtagat ctgatcggcg cagcaagtcg ggcacccgca gaagcggcag ctcacttttt
    26401 gatacccggc agatctcgcc aaggaaaggg gtgagcctgt ccatttcagc gatggcgaga
    26461 tgcacgagac ctgccaggat gttttcgtcg tagtggccgg tcgggaaaat gtttgtcacc
    26521 cggaacatga ccctgccctc gtcccagtcg agttcgaagt tgccaacgtt gagctccttg
    26581 ttgacgcgca tgagcatttc cgccgcgggc aggcggtgcg tcctcggcac cggcatggcg
    26641 gcctgtgcca ccacgctgat gatgcctgca tccccgtatg attgcgcatg cacgggaatc
    26701 ttcgcatgat gcgcttcgaa ggccatttcc accacttcca tgccagacac ctccgtgtga
    26761 ctccagcctt gcgctttgaa ggctttgagg gcggcggcgt gaagggcgga cattggcatg
    26821 ggaagggcgg cgccggggat gccggtgagg gtcggggcgg ccagattcga actgacgact
    26881 tcttgctccc aaagcaagcg ctctaccagg ctgagctacg ccccgagtcc gacaccgggc
    26941 tcagcccggc ggagggaagc ggatactggc gacgaatggc cctcgcgcaa gatgaaagac
    27001 cgtgcattcc atgattggcg gaaaatttgc cccgacggag gcagccgggc gcgcatgggt
    27061 tgcagtgaat gagttcacgc attcacctgg attgagatga agttgatgtc agcggaacag
    27121 ctcgcggaaa aattcacctg tctgccggcg ggcattgtcc gtccagttcc aggcgcggcg
    27181 ggcggtgcgc ttcaccgagc gggcggcgcg ctcgccttcg gagtcaggct gcgagtcagc
    27241 cgctgcgcgc acgcgcggcg tccagtcctg gaagagcacg gcgccgtttt ccgccgagac
    27301 tacgacaaag ccggtttcgt agccggtctc atccaacagg gccagtcccc attccggcgc
    27361 gccagtttcc gcgttggcgg ccaattgata gtcaaccgag acgaaagtcg ccttcgccag
    27421 agccgcggct ttggcggcgg tggcgcgggc cagggaactg cgaacgcgaa tgcggctgga
    27481 atccagcgaa cggggaggca ccctgtccag gatgcgtgat cccgctccat cagccgaggc
    27541 tacccatgcc ggtccttcga ggcgcactcg catgcgcaga atgtcctttg gtcggaatgc
    27601 gtcgcgcgaa tagaccgtcc actccacggg atctgacaca gctccagcgc ctctcaaaag
    27661 aaccgcattc tcccgcaccg tgccgccaaa ctggcgtgcg gcggcatcca ggatttccgg
    27721 gtttgcgtgc agactgcctg agaaggccag cgcgaggatc gggagaataa agcgtgaact
    27781 catggcctcc aggctagcga tgtcatgccg gcattcaaca agcgcttgga tgaccggggc
    27841 cgcgcgagct tttcccgcag ccaactcccc cttgaccatc caccgcccat gcggcagtct
    27901 gcgcgccctc aatccagaac cacaatccat gcccgccaaa atctacactg aaaaggacgc
    27961 cagcctggct cccctcaaag gcaaaacctg cgccgttatc ggcttcggct cgcagggcca
    28021 tgcccacgcc ctgaatctca aagagagcgg cgtgaatgtc atcatcggcc tctacgcagg
    28081 ctccaagagc cgcaaggtcg ccgaggagcg cgggctgaaa gtctatgaca ccggcgaggc
    28141 cgtgaaactg gccgacgtca tctttgtcgc cgtccccgac accaaaatcc ccgcttgcta
    28201 caaggccgac attgagccga acctcgccaa gggcaaaacc ctcctcttct cccacggctt
    28261 cgccatccac tacaagaccg tcgtcccgcc cgcggatgtg aacgtcatca tggtggcccc
    28321 caaaggcccc ggccacatcg tccgccgcca gtacaccgaa ggcaagggcg tgccctccct
    28381 catcgccatc catcaagacc cctccaagaa gtccaagcag atcgcgctcg cctgggcgca
    28441 cggcatcggc ggcacccgcg gcggcgtcat ccagaccacc ttcaaagagg agaccgagac
    28501 cgacctcttc ggcgagcaga ccgtgctttg cggcggttgc agcgccctgg tccaggccgg
    28561 cttcgaggtt ctcatcgagg cgggttactc ccctgagatg gcctacttcg agtgcctgca
    28621 cgagctgaag ctcatcgtgg acctcatgaa cgagtccggc atcgccggca tgcgtttctc
    28681 catctccgag accgccaagt ggggcgatgt caaagtcggc ccgaagatca ttgacgccag
    28741 cgtgaaaaag cgcatgaaag ccgcgctcaa agacatccag accggcaaat tcgccaagga
    28801 atgggtcaag gaatacgaag gcggctacaa ggagttcaac cgcctcctca aggaaggcga
    28861 gaagcacccc atcgaaaaga ccggcgcccg cctccgctcc ctcatgccct ggatcaaaaa
    28921 gcgcgacacc aaaggccagc aggccagcta cgagtgatca cagggtcctc gcagacctcc
    28981 cctctccagc gcgggccgca aggcccgcgc tttttgttgt ggagttcaat ctccaggttg
    29041 ttgcaattcc tcgctggata gatttccctt aaaagcctct gcgaatggtt tattgtctgt
    29101 ttttgaaaaa actcagaggc aggactcacg aaccatcaga ggtgaatgct cttgactctg
    29161 cgcgcgcctt cttgccactc gatgcccttg cccccgctac cacaccgcta acgaaatcgc
    29221 cgacaagatc gaatcgcatc aaaaagctcc cgagatgaca gtggcgaaca ttgagtcgat
    29281 tgaatatgat tccagacaca ctcaattcca atctgcgccg ttaacatctg cttcacatgc
    29341 cccgctttcc gctccatgac ctcagcgaca ccgagttcga ggcactcgtt gtgctgatat
    29401 gccgcgaact catgggcatt ggtatcacct ctttcgctcc cggtcgggac ggcggaaagg
    29461 atgccaagtt cgaggggacg gcaacagcgt tcccaagcac cacagccccc gcatcaggaa
    29521 agttcattgt tcaggccaaa cacatctcgt ctcccgtggc ttcctgttct gattacgact
    29581 tcgagaccac gctgattgac aaagaaatcc caaagatcac tcggatgctc gaagagggct
    29641 gcctgacgca ttacctgata tttaccaacc gccgtaaaac cggtggagca gaagaccgca
    29701 tcacggcgca catcaagacc gccaccggcg tccagcatgt ctggttgcgc ggcttggagg
    29761 atattgagcg tgaactcctt ctgcaacctc acatcgccaa agcggtcggc ctcgacaagc
    29821 ttcgctcacc tatccagttc gtcccggagg atctgaggga tgtcatcctc gccctccacg
    29881 ctcaccgcca gtccctgccc agtgcgtttg atagcgaaca tgactttcgg gactatcccg
    29941 gcctggacgc caaaaacgaa gtcaacggac tcacccaagc ctacgacgac tacatcaagg
    30001 aaaactcaat gccgcatttt catgccatcg aaatgttcct gaaaaacccc cgcaacgaaa
    30061 ccctcaagga gcgatatcac accgtagccg acgagttgaa aggccagctc atcgttcatc
    30121 gtgagcggtt cctcaatttt gacgacgccc tggaaaccct ctacaaccta atccacgaac
    30181 gctgcccgga aatccaatcc gttcgccgta tcgtgaaaat catcatccac tacatgtatg
    30241 tgaactgcga aatcggccag aaagcatgat ccgccccgcc aaacatttga atctcaacac
    30301 ctgcgtgctc cgcgccgcca gccgtctgct ggcgcgattg caacgggagc ggatttgtcg
    30361 tttcaccgac ctgcgggcca ccctggccga tcttggcgac gatgccgagg ttgtctttct
    30421 cccgaccatt catttcctct acctcgtagg ccgtttggac taccatccgc agaccgattc
    30481 cttcgaatac cttcaacccc ggaaaggtga cgccacatga agctcagcca cctttattca
    30541 gatcaacccg agttattccc tcctatccgc tttcgggacg gcctgaatgt cgtcctcgct
    30601 cacatccgtc accccaagga cgattccaag ctcggccaca atctcggaaa gacgcttctc
    30661 atcgacgttc tcgatttctg cctcctcaag ggcatcaaca aggaccacct tttcaagcgc
    30721 cgtaccgatc tctttgccag gttcgtcttc tttctcgaga tccagcttcc cgacggtagt
    30781 cacctcacca tccgccgatc cgccgccgaa actaccaaga tcgccttcaa gcgccacgag
    30841 caagcctggc agaacttctc tggactgccg gaatccgatt gggatcattg gcgcgagaca
    30901 tttgacaacg ccgtcaatct tctggacagc cttctggctt tcacaccgat caaaccttgg
    30961 ccctaccgca aaggcatcgg ctactttctc cgtggccaga acgactacgg aaacgtcttt
    31021 cagctcacca agttcggagc cggcaaacac aaggattgga agccctacct tgccagagtc
    31081 ctcggttttg atgacgccct gctcaccgag aaatacgagg ccgatgcagc ttcgacaaag
    31141 ctccacgccg atgccgccga actccaggct gaaatcacac tcaaacccaa ggacttcgac
    31201 aaactccgcg caagcatcgc cgtcaaacgc gatgaagtgg aaaccaagct ccgcgctctc
    31261 gacgccttcg attttcacgg ccaggaaacc gatcttgctc gcgaagcggc ggaaaccatt
    31321 gaaacggaaa tcgcagcaaa caacagtctg ctttacaacg ccagacatga cctcgcccaa
    31381 atcgaacgcg gacttgagga cgacgtccat ttcgacctcg ccgatatcca gcgcgtattc
    31441 aacgaagccc aactctcttt ccccgaccaa ctcgtgcggg actacaatga cttggtcgaa
    31501 ttcaatcgcc ggattctcac cgaacgccag actgccctgc gcgaccgtgc tgcggcccta
    31561 cgtgcggaaa tcgccgtcgt cgagcaggca aacgccgctc tatcaaaccg tcgccgggaa
    31621 atcatgcggg ttcttggcgg caccgacagc ctgcaaaaat tcaaggacct ccagcgccag
    31681 accgacctgg accgcgccaa tctcaccctc atggaaacca aagctgcgaa gctggaaagc
    31741 ctcttagccc tccaagataa actccgccag accaaggccc gtgccgaaga acttactgcc
    31801 gccattcgtc agatggtcca tcagggcagc gcgcgctacc aggagatcca gcgcactttc
    31861 agccgcatca tcaaagaagt gacgcatcgc accgcgctgc tctacctcga ccaaaacgga
    31921 gaaggtaatc tcgacccaca tgccgaattc agcgatgccg aaaccgatct tcacaccgag
    31981 gaagaccggg gcacatcgtt tcgccagttg ctttgcatcg ccttcgacct cgcggtcctc
    32041 atcagctacg ccaaagaacc cttcttccac ttcgtctatc acgacggagg cctcgaacgc
    32101 ctccaaaaca agctcaagct ggcccttctc cggatcatcc gcgaaacttg cgccacacac
    32161 ggcatccaat acatcttcag cgctctcagc gaggatatcc cgccccagga agacagcgct
    32221 ggcctatgcc ccgctccggg tgaagtcatt ctcactctcg acgacagagg cgactcaggc
    32281 cgcttattca aaatgggcac cttttaactc gtcgtggaat ctcagtgatt ttgtccatct
    32341 ctttatcgct tcccatcaaa cccgcaaggc tatccgtcga attgagctga acgatggtga
    32401 gatgacttgt tcgcggcatt gtttccgcca gaagaccggg gtatcaccaa gaaatgaatg
    32461 tgcgtctgat ttgatcaccg tcccgcaaaa ggattgccct gtcttccgag gaaagcggga
    32521 tgagcctgtt ccatctctca tagttgatcc agacaagccg gctttgcgcg gtttcaccgg
    32581 tgccggattt tgccggggac gagattctca aggcatacat cgtcgtttcc agattgatga
    32641 acatttgcgc tcgtttaatc cccgccaaat ctaccaattc cccgagtgtg cagcctctgg
    32701 gcaaacggta gcttcccggc cgctcaaacc cttctgcaac tgtgacgaga atcaaatttt
    32761 cagactgctt ttgctcagtc tcgaaaccat ccagagtctt tttttctgtg ataacagagc
    32821 atccagccag aagccagacc agcgcagcgg ggagatgaga ctttctggga gatcgcttca
    32881 cgacacgtct cttttctcat gaataaaacg accgtcaata ttgccggtga gggaattttc
    32941 cgctgtgtgt gaacaatcgt tcgtttcagt cattcatctc ggtccacata tcgcggtttc
    33001 cagccatgac ctctcttggg agcagcttcg acgccggagc attcattcac gcaagctctg
    33061 cttcgcactg cgggcgcacg atagaatggc gcggtgctgc ggctttttgc cgctgggttg
    33121 cggcccggaa ccgcgctcac tctgtttgcg atccgcgtcg tcgcacctct gatctgcggc
    33181 ggagcaccgt tttttcgcag cctcgcagat atggcagtgc gagcccgcag ccaggatatg
    33241 cgaacgtgcg gctcggaggt gcgggttttg agaaatttcc ctgatgttgt tgcggatttt
    33301 gggtccgcgc tcgcgtctcg gagctgcgag cgcgcgttcg caaacttcgg gcgagcacct
    33361 cgcctgccgc agcctcgcgg ctcggagccg gaggtcttgc acctcggaga tgggagaccg
    33421 gaaaaaaggg gtgcgtccgc gcggctcgga ggtgcaatgc cgcgcctcta agccgcaagg
    33481 gcgctcatcc gaactgcggc ggcgcggatg ggagatgcga gagagaggca cgggcgtgcg
    33541 cggaaggact tgatcgcacg aatttttgcg gagccatttg tcagtccctg tttcgcgttt
    33601 tctggccgga agccctgacg ggaaaatggc cggagaagaa ggggtggctt cgatggattc
    33661 tttttggaaa ggtcggcttt ctggggatgt tggtttcacc ttccatgtcc caaccctcca
    33721 ttctcgtcat cggctgcggc agcattggtg agcggcatct gcgctgcttt ttgaacacgg
    33781 gccgctgccg cgcggcggtg tgcgacacga atggccgcct cgtggcggag gtggcggggc
    33841 ggtacggggt gccgggtttt gagagcctgg aggcggcgca ggaggctttc ccggcggatg
    33901 gctgggtggt ctgcacgccg gcgcacacgc atctggcgct ggcgctgcgc gggctggcgc
    33961 ggggcgttca ccagctcatc gaaaagccgc tcgccacggg tctggagcag gtggaggaaa
    34021 cgcgcgccgc cattgcctcc agcggctgtc atgtggcggt ggcctatgtg tttcacgcca
    34081 tgccctggct gcgcgccatg cgggaggtga tcctctccgg cgagctgggc agcccgcgcc
    34141 atgccacgct cacggcgggg cagcatttcc cgaccttccg cccggcttac cgggagattt
    34201 attatgcccg gcacgagagc ggcggcgggg cgatccagga cgcgctgacg cactcggtaa
    34261 acgccctgga atggctgatc ggcccggcga cgcgcgtgta ttgcgatgcc gcgcaccagg
    34321 tgctggaagg cgtgggcgtg gaggacacgg tgaatgtgtg cgcgcgccat gggagtgtgc
    34381 tggcgagctt tggctacaat cagttccagg cgccgaatga gttggaaatc cgcgtccact
    34441 gcgagcgcgg cagtgtggcg atgcaactgc acgagcagcg ctggggcatg atgcggttcg
    34501 gtgaaaagga gtggacctgg cggcgccatc ccaagcccat tgagcgcgac gactttttca
    34561 cctggcaggc cgagtccttt ctcgaaggca tggccggcag gccgtgcttc ctcgccactt
    34621 ttgaggaagg tgtccagact ttgaagttca acctggccgc gctggagtcc gcgcgcagcg
    34681 gtcagcccgt cgccatctca tgagcgctga aatccgcctc gccatgctgg gcatgatccc
    34741 cggcaacggc cacccctatt cctggtccgc catcatcaac ggctacgatc ccgccgccat
    34801 ggccgcgtgt ccttacccag ccatcccgga ttatctgggc aggcagcctt tggagacggt
    34861 gcgcatcccc ggggcgcgtg tgacgcatct ctggacggac aatcctgcgg acgctccgct
    34921 ggtggccaag gccagcctca tcgaaaacat cgtcgcccgg ccggaggagg tcatcggcca
    34981 ggtggacgcc gtcgtcattg caacggatga cggcgatgac catgcgcggc gcgcgcggcc
    35041 tttcattgag gcggggctgc ctgtattcat agacaagccg ctggccacga acgtgccgga
    35101 cctgcgccag ttcgcgcgct ggcagcggga gggccgggtg ctgctctcca ccagcggcat
    35161 gcgctacgcg ccggagacgc ggctgagcgc cgaacaaaaa gagcagatcg gcgacccgcg
    35221 ctggatcacc agtttcacat gcaagacctg ggagcgctac ggcatccatg cgctggaggc
    35281 ggtggctccg ctggtggggc cggggtttct gacggtgcgg gcgcattcgc agcccggctc
    35341 ggatgtggcg cacctcacgc accgctcagg tgtgcaggtg acgctggcgg cgatccacga
    35401 cgcgtatggc agcttcggcg ccgtgcatct ttatggcacg aaaggccaga tcccgctgct
    35461 tttgcgcgac acctaccacg ccttccgcgg ccagctcacc gccttcatcg agatgctgcg
    35521 cactggccag ccgccgctgc ctttcagcga gacggtggag ctgatggcgg tgctcatcgc
    35581 cggaatccag agccgggaaa aggggggtgg tgttgtcgag gtcgcggaag tgctgaaaag
    35641 tttggagggg tagcagtggt tcctggttcc tggtttgaac ttcgattttc caacctcgga
    35701 ttgattcgcg ctcatttgtg tttcttttgc ggcatgacct ctctcgaccg attcagcctc
    35761 aaagacaaag tcatcctcct caccggcggc gcggggcttt atgggcgagg gctggcggca
    35821 gacctggcct cggcgggcgc gcgcctggtc attgcggcgc gtgacaaggc gaagctggag
    35881 gccgtggccg ccgaggaaag ggagcgcggg tttcaagttg aggcggaatg cctcgaccag
    35941 ggggatgagg cctccgtcct ggctctgcgc gaccgcgttc tggcaaggca tggcagggtg
    36001 gacggcctgg tgaacaatgc cgtggcgcgc gtggtgaaag gcgccgccgg aagtgtggag
    36061 cagtggcagg agtccatgcg cgtgaacgcc accggcgtga tgctgatgca ccgcgtcttc
    36121 ggcgcggaca tggttgagcg cggcatcggc agcatcgtca acatcggcag cattcagggg
    36181 atgatcgggc cgagctacga gttgtacgcg gggaccagca tgggcgacct gccgccggat
    36241 tatttcttcc acaaaggcgg catgatcaat ctcacacgct tctacgccgc ccattttggg
    36301 gcgcgaggcg tgcgtgtgaa ctgcctgtct ccaggcggct tcttcaatca acagccggag
    36361 ccttttctca gccggtactg cgggcacacc ctgcttggcc gaatggcgga tccgtccgac
    36421 ctcggtggtg ctgtgatctt tcttctcagc gatgccgcgc gctacatcac gggtgtgaac
    36481 ctgcccgtgg acggcggata cacggccaaa taaaaacgcc gcggaccttt ggggtccgcg
    36541 gcgccgtggg tttgcctcag cgtccgaagg aaaagaacca gctcacactg cgggaaggca
    36601 ccggtgcgca aacgggcggg tgataaacca ccggcggcgg gcagacaggc cggtgaacca
    36661 ccacgggcgg gcggcaggcg cggcatccgc atgcgctgtg ctgggtcacc cagcgtccga
    36721 cgggctggcc ccagcggtcg cggccataaa cttgatacac ggcgtaaaca gggcgtccgc
    36781 atggcaggta actgaccacg cgggagtgtg agtggcggcc gtcccaggct tcggcggcgg
    36841 tgggggctgc cgcgccaaga gcggcggctg cggtgaggat gaggaggatt tttttcatgg
    36901 tgctgaactt gtgttcagcg catctgacgg cgcttcattt tgcctattcg aagctccttc
    36961 aattttttcg aacaggcccg gtggtgtcag cgcaggtgct gtttgaggaa gtcgaaagtg
    37021 cccgcgccat ggatggtgtg aggaccgtcg aagtgctcga tgacggcgcg cccgccgatg
    37081 cgcagcttgt tgtagaggcg ccgtactttg gcgaactcgt agttcaccca ttcgtcaatg
    37141 ccgacgccgt cgttgtggcc gcgctcgacc atgaagggcc gcggcgcgat gagcgcggac
    37201 atctccgcgt aattgtaagt tccgccgaga ttccactccc agatttcata ctcaccggtg
    37261 aacacataac tcaaaggcag gtccgtggtg gcgcacttgc gcacccattc attgaagtcg
    37321 cccgagcaga tgctcaggca gtaatccgtg agcacggaag gcgtgcgcat ggcggacttg
    37381 ccgccgtagc tcagaccgta aaaggcgatc ttgtccgcct gcgtgaaggg ccgcgccttg
    37441 agccactcga tgatgcgctg gtgctggccg ttgatgatgg agtagagcgt catccccagc
    37501 ggattggcct tccgctgcaa tgaccggaag gcgtccttgc cgcggtaaag attgtggggc
    37561 gcgaaggtga tgaacccctc ctccgccagg cgcagcgcga aggctttgta ggcggcaaag
    37621 gcgcgggtgc tttcatcggt ctcgtagcaa tgctcgggca gtccctccag gccgtgctgg
    37681 cagacaatga cggggcgttt ctctccctcg cgcaaatcct tgggcagagc cagccagccc
    37741 caggcgaaga catcctgcca gacatccagc acgacttcat ggatcaccac attttcggtt
    37801 tccctcacca cccggctgcg cgggttagcc gggacgccgg gatcaggcag gcggccgatg
    37861 acctcgttcc aaaaccgtcc gcgctccacg gcggcgcgct tttcataatc ctccagcgag
    37921 ttcagcggaa gcggcttcca aaagccggtg ttgcgctcgc tttcgatggt ggccaggaga
    37981 cgctgggtga acgactccat ctcgcgcacc tggcgttttt gccgcgcctg gaccgcatcc
    38041 acgctggtcg tctgagcggg cacgcgcgga gcgtccctgg ctgcggccat gcgcgccagc
    38101 atcgcccgcg ccgcagtctc cggaaaaaga tggccgatga tttcagcggc gctgcttttg
    38161 cgctcgaaga ctcggcccca ctcttgcgcg agtccattcg tcaactccag ggcgcgggcc
    38221 atctcggcgc gcgcgcttcc cagcggaggc gtctcgatga agcccggagc ggccaccgcg
    38281 cgcacgccct tgcctgcctc gggcggtcct ttgacttcgg gaaagccggc gtgctgcacc
    38341 gccagatgac gcggcgcgat gagcgtggcg agttccgcgt cgccaaactc ccgcagcagg
    38401 ccgaagacat tccggtagac gggctcctgc cagagaccct cgcgcggcgc gaagtgcccc
    38461 cacacataga ccgagccgac gcgctcatcc acagcgcccg cgtaaagcgc cagcaggccg
    38521 ccctctccga ggcccgccac gcacaccggc cgccctccct cctgctttcc gaaccagtcc
    38581 accagcgcca gcactttttg gatttcatat ccgatgacat gccggccgag gatgaaggac
    38641 tggcggtaaa tccactcgcg atgcggcgcg ttgatccgaa tgcccaggcg ctcgctgccg
    38701 gaaaaatccg cgtcacggct cacgagcacc ggaatgagaa tctgcgcgcc ggcattcata
    38761 agcgcggggt ccagcgcgcc gtctttgaag ccgagaagct gctccggcgt ggtgtccgcg
    38821 tcaggcagat agacgacacg cgccagcact tcacctttcg gctggatcag caggccctcg
    38881 ccatgcatac cctccagcac aggccagcgc acgcgatgca cggaggcgtt ctcgttttcc
    38941 gcgatgagcg acgggctggc agtgtctgcc acgaactcca gcgaggaaaa tggcgcgcgc
    39001 tcatccacca cgccgagaat gcccctcagc ctttcgcggt tcgccgccac ggaggcggag
    39061 taggcttcct ttgaagtagt gtcacgcttc cagaaggtct cgcgccgcgc cgccgcagcc
    39121 agagtctcgc gcaatagaaa gcggtccgcc ccggcgacca tcaatgcgga aaaatccgct
    39181 tcagccgcga ggggttgagt tccaggcaga tgggcggcga cggcggctag caaaatggac
    39241 aggttcataa tgtccagtat ctacgcgccg gcggaaggcc ctcacacctg gatgagggtg
    39301 aaatccgcgt tgacggcagc ctgtccggcg atagtctcgc gcctcgcatc aacgcatcat
    39361 catttatcaa tacatcacaa aatccagcca tgtcccgctc ctacatcttc tcctccgagt
    39421 ccgtcggtga aggccacccc gacaaagtcg ccgacaccat ctccgacgcc atcctcgacg
    39481 catgcctcgc cgtggatccg aggagccgcg tggcctgcga gacctttgtg aagagcaacg
    39541 tcgtcgtcgt cggcggtgag atcaccatcc ccaagctcca gaacaaaaaa ctcggcacca
    39601 ccaagcccat tgatgagatc atcaacatcg gcaaggtcat ccgcgaggct gtgcgcggca
    39661 tcggctatgt gaatgacgac gacattttcc acgccgacaa aatcttcatc aacaactacc
    39721 tcaccgccca gagtcccgac atcgcgcagg gtgtggacgc cgagaaggcc gagggcaaaa
    39781 agaccgccga gcagggcgcc ggcgaccagg gtatcatgtt tggttatgcc tgcgatgaga
    39841 cgccggagct gatgcccgcg ccgatcatgt acgcccaccg tctcggtcgt gagttgaccc
    39901 gcattcgcaa ggcaggcaaa caggccaaat ggctgcgccc cgacgccaag tcccaggtct
    39961 ccgtggagta tgtggacggc aagcccaccc gcatcgtgaa cgtggtcatc tccacccagc
    40021 acgccgccga tgtcagccat gccgagatcg agaaattctg cattgagcaa gtcatcaaaa
    40081 aggtgctgcc caagagcatg ctcaccaagg acaccgagta cctcatcaac ccgaccggca
    40141 agttcgtcgt cggcggcccc cagggggaca gcggtctcac ggggcgcaag atcatcgtgg
    40201 acacctacgg cggcatgggc cgccacggcg gcggcgcctt ttccggcaag gatccctcga
    40261 aggtggaccg cagcgccgcc tatatgggcc gctgggtggc gaagaacatc gtcgccgccg
    40321 gacttgcctc gaagtgcgag atccagttcg cctacgccat cggtcacccg cagccagtca
    40381 gcgtgcatgt tgacaccttc ggcaccggcg ccgtgagcga tgacaaaatc ctcgccgccg
    40441 tgctcaaggt cttctccttc aagcccgccg acatcgtcaa gcagctcaac ctgctgcgcc
    40501 cgatctactc gaagagcacc aactacggcc acttcggcaa ggacgacgcc gacctgacct
    40561 gggaagccgt caacaaggcg gacgcgctca aaaaagcggt cggctgacct ctcgaaagct
    40621 ccatccaccc gcccgcgctg gaggaatccg gcgcgggttt ttcatgcgcg ttgctagcgt
    40681 tcgtcagcca ccatcttctc gatccacttg ccgaggtcgg gagcttggaa ggcgcgcatt
    40741 ttttccagca gcagcgcggg atcgcgctcc accagcacgc agtcgcgatg cgcgggcttg
    40801 aggaagccgt ggccgcgcat ctggtcgaga aaagcgagca gcccgtcgaa aaagccctcc
    40861 gcgttcagca ggccgaccgg cttgccatga aagctgagct ggagccaggt gagcgtctcg
    40921 aaaagctcgt cgagcgtgcc aaagccaccc ggcagggcga tgaagccgtc gctgaggtcc
    40981 accatcatct gcttgcgctc gtgcatcgag cgcgtgacat gcagagcggt cagtcctgtg
    41041 tggcccagct ctttctccat gagcgaatgc ggaatgacgc cgatgacctc gccccccgcc
    41101 gccagcgccg cgtccgccac cgctcccatg aggcccaccc ggccgccgcc atagacaatg
    41161 ccgatgccct gccgagcaag aaaggcggca agcccggcgg ccgctttttt gaaaaccgga
    41221 ttgtgtccgc cgttggcccc gcagtagatg gctatgcgca tggcgctcca ttgcgcgaag
    41281 ggcgcgtgga atcaagctcc gacagtggaa tcaagctccg ggaactcccg gcaccagctt
    41341 tccggctttc cttgccgtgg tgatgaaggc gtcctgaaga taaagaggct ccagcgggcg
    41401 cgcggcgcgg cgctccattt ccgcgtcatg gaatccctcc gcgatccgcg ccagcgccac
    41461 ggcgtc
//
LOCUS       DAOMYJ010000019        40242 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Prosthecobacter sp. isolate AMR_MDS_5629
            NODE_380_length_40242_cov_21.997910, whole genome shotgun sequence.
ACCESSION   DAOMYJ010000019 DAOMYJ010000000
VERSION     DAOMYJ010000019.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867850
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prosthecobacter sp. (wastewater metagenome)
  ORGANISM  Prosthecobacter sp.
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Verrucomicrobiaceae; Prosthecobacter.
REFERENCE   1  (bases 1 to 40242)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 40242)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.30
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:34:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,742
            CDSs (total)                      :: 4,684
            Genes (coding)                    :: 4,676
            CDSs (with protein)               :: 4,676
            Genes (RNA)                       :: 58
            tRNAs                             :: 55
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..40242
                     /organism="Prosthecobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_380_length_40242_cov_21.997910"
                     /isolate="AMR_MDS_5629"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1965333"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..410)
                     /locus_tag="PLP58_03520"
     CDS             complement(<1..410)
                     /locus_tag="PLP58_03520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009963233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine protein phosphatase"
                     /protein_id="HRJ07624.1"
                     /translation="MQTNKDTARATVRLGYDGRVHKTFKGHLARERFEHEVRVLRYLE
                     KMGCPFVPRVLDTDPERLYMVTSNCGQRVERMTEDKLRQIYAELEQYGVRHEDPYLRN
                     VTYRASDGRFCLIDFEFATILSQPDAGPPPPEPDP"
     gene            complement(516..1622)
                     /gene="rsgA"
                     /locus_tag="PLP58_03525"
     CDS             complement(516..1622)
                     /gene="rsgA"
                     /locus_tag="PLP58_03525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018969271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HRJ07625.1"
                     /translation="MTLQHLGWNETFAAEFEPYLERGWTPARVSSDNKISCRLLGVED
                     GRIEECDAVLAGKVYHEAETDAELPAVGDWVALDKSGDENVIRARLHRQSRFSRKAPG
                     NSSEEQVIVANASRVVVVTDPGTDFNPRRLERYLALIARGGAEAMVVVNKSDLHPAER
                     CAEVIGAVRDLGGGVEVCLTSVLENKGFKTLKQHLKKGATLAFTGSSGVGKSALINHL
                     IGEEWLWTGEVNEVTGKGRHTTTARDLLVLPRGGILVDNPGIREVQMWTDEETLRGRF
                     ADIDQIAARCRHHDCGHGRDAGCAIRAAVEGGELSPERYKAFLKLEDEIQELRRRRKK
                     RQMTLERRAKRDHKIKARNREDRREIEWELKPRP"
     gene            1716..2012
                     /locus_tag="PLP58_03530"
     CDS             1716..2012
                     /locus_tag="PLP58_03530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ07626.1"
                     /translation="MKANIPTLEQLKRAAAIAEQITRLEDELQAIFGNAKPGRKKKSA
                     LDAAPAAAPVAAKRGPKAKKAKRVINFSPEARARIAAAQKARWARFRKDKKAEG"
     gene            2156..3574
                     /locus_tag="PLP58_03535"
     CDS             2156..3574
                     /locus_tag="PLP58_03535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ07627.1"
                     /translation="MRTRFPFKYGIASMSALPHLFVSVGMRVDGRQVTGLSSEGLPPK
                     WFTKDPSTTFEHDLAEMLSVIQNAARVAGNAAEKPVRFFPWWKETSEEQKRWAGLRRL
                     PALLSNLGVSLIERAVLDGLCKSGAVPLHVFLKSDEAGIDLGGVRPELHGMRVAGCLP
                     ESPLSNVAARHTVGLGDPLRTSEIPGDERLDDGLPQALDDCIRSYGLRWFKIKICGRE
                     ETDIPRLREITRVLAEGCPSGFQATLDGNEQFQSLADFREFYRLLHADKALAPLFDSL
                     VFIEQPLHRDHALRDDVASVLEAWKDGPGIIIDEADGSLDDLPRALDLGYSGTSHKNC
                     KGIVKGLANSALLQKRAAFMTRAPILSAEDLANVGPVALLQDLAMVAALGIPHVERNG
                     HHYFRGLGMHSPGVQEEVLRAHAGLYRRHERGFAALDIRDGLLDLASVNSAPFGCAIQ
                     LDPARFTPLDAWVKGGGLAELG"
     gene            complement(3576..4955)
                     /gene="mpl"
                     /locus_tag="PLP58_03540"
     CDS             complement(3576..4955)
                     /gene="mpl"
                     /locus_tag="PLP58_03540"
                     /EC_number="6.3.2.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018969153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate:L-alanyl-gamma-D-glutamyl-
                     meso-diaminopimelate ligase"
                     /protein_id="HRJ07628.1"
                     /translation="MTPKHIHFIGICGTAMGSTAAALRAQGHIITGSDEKVYPPMSDV
                     LRAAGITLSEGYKAENLPAGADLHVVGNAISRGNEELETVLERRLPYISMAELIKREV
                     IQGRRSFVVSGTHGKTTTTTLLAWLLEHAGRNPGFLIGGVPANFESGARFNDSEFFVI
                     EGDEYDTAYFDKRSKFLHYLPECVIVNNIEFDHADIFRDLDDILLTFQRLLNSVPRNG
                     LVLLNGDDANCLALKSPAPVRTVGLGPDYSERLVITGGGPESTDFTLNGAAFSLPMIG
                     EFNARNAAMAVCAARHAGLSDEEIRAGLASFKGIRRRQQVRGEAGGVTVVDDFGHHPT
                     AIRETLRGMRRKYPGRRLWAVFEPRSNTSRRNLLQNELIAALREADGSVVASVANPEK
                     VPAELRLDPDKVAASVTSAGRACFHEPDTAAIVARLKQETRPGDVVIVFSNGGFDGIH
                     DKLLAALQA"
     gene            complement(5042..5650)
                     /gene="thrH"
                     /locus_tag="PLP58_03545"
     CDS             complement(5042..5650)
                     /gene="thrH"
                     /locus_tag="PLP58_03545"
                     /EC_number="3.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007418286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphoserine
                     phosphatase/homoserine phosphotransferase ThrH"
                     /protein_id="HRJ07629.1"
                     /translation="MKKQTLVTLDLEGVLVPEIWIAFAEKTGIDALRRTTRDEPDYDV
                     LMRGRLAILDEHGLALPDIQEVIGTLAPLDGARDFLDELRALTQVVILSDTFEEFARP
                     LMRQLAWPALFCHHLETDARGRIVNYRLRQPNQKQKAVAAFKSLNYQVVAAGDSFNDT
                     TMLGEADTGFFFHAPESIQERFPQYRAFERYEDLLEAVKGCL"
     gene            5771..8698
                     /locus_tag="PLP58_03550"
     CDS             5771..8698
                     /locus_tag="PLP58_03550"
                     /inference="COORDINATES: protein motif:HMM:NF016146.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease"
                     /protein_id="HRJ07630.1"
                     /translation="MTAGRARSRMLGALAALLVMPCPAFAQTQPAPQSLPCTQNFGSA
                     TFTTAPAGMAAWNGLNGGGITSAAQAANSAPQGDAAISAATAAQTTGGCYGLMTGGNA
                     RFYIQTSSNATNGVNQLALALDTTGWTNVALSYDVEIINAKPRTIGVLCQYRVGTSGA
                     WTSLAAASGSNPYSQAGGTAGVKTSPQITLPPPAENQPVVQIRWAVWRGTETGDSSGA
                     AIDNISVSGSAAGAVLSASVVPGMVAESDGAGAAQLTVSRTGSTDAPLAVTLAISDTG
                     ALAYDGPNPFPIPAGQSSATVPLRAVDNDVLDGDKTVTLTVTAPASVAAQATIQVLDD
                     EDAFSPPPGYYADAVGLSGSALKAALKIISSPATYNSYAYSDTFNPLRSLDQDPASSG
                     NVVTIYSGASLGKNAVYFPGGPDADSSWSREHIWPDSYGLDPDNANPGAPDGGDAGPD
                     FTDLFNLRPCVHSVNVLRGNRYFDITTGTPSIPALAPLCSYDSNSWQPRPEERGDIAR
                     AMFYMAARYDGGDPLTLDLELSNTPNAAAGRFGRLSTLLRWHEEDPVSLEERQRNHRI
                     YTTYQRNRNPFVDRPGFAAEVWGHIRMDKTAATVTEGQPGTDAVLSLAAAPAADVTLS
                     FQTSPAGQVSVSPPSLTFTPANWDQPQSLSISAVDDSLFEAEMTVTMSTAITTADTRY
                     AALEVPPVSVTVISDDPLIQPAALPLSHGGPWSPLPSGFLGEGIGAPYGSSLGGDTAA
                     GSARFDTTGARLVIGFNAPAETLTYHLRGNPADGSATSGSFHIRHSADGLSFTSLRVV
                     TDKNNTDQLFTDSVPRSARYLEFIYSQKNSGNIQLDKLGITAAPSFISWAAARDLSGV
                     NAGMLVDAELDGFTNLAEYALGGHPLQHDAAAITPAASRAGGHLRITAVIRADDPDLG
                     VQAVTTADLGMPQSWTATGVVQIQPVDQSGVPPGFERVTFQSPLTGGRAFMRLVISLQ
                     GGG"
     gene            8755..12138
                     /locus_tag="PLP58_03555"
     CDS             8755..12138
                     /locus_tag="PLP58_03555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009965414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1553 domain-containing protein"
                     /protein_id="HRJ07631.1"
                     /translation="MTVFHRLLISTACLGAASGAEVDFARQIRPILSENCFFCHGPDD
                     KKREADLRLDDEAAAKKNNGGVIAVAPGDPEKSALIQRITSTDPDEIMPPPKQHKTIS
                     PAQVALLKEWIKQGAKWGKHWSYEKVVRPKVPAGAKNPVDAFLAARLKKEGLSFSPAA
                     DRATLIRRMAMDLTGLPPTLEELARFSSGTSNPSDWSGRVISHYLSKPAHGEHWAAQW
                     LDLARYADSSGYPSDQPREIWAYRDWVIRALNVNMPFDQFTIEQLAGDLLPSPSDDQL
                     IATAFHRNTMTQNEGGTNDEEFRNAAVIDRVNTTFAVWMGTTMACAQCHTHKYDPITI
                     EEYFQFHAFLNQSADSDKKDEVPVHSFDTPEIKKQRRTLQAEIAALESKFSQPPPEWL
                     AGLDAWDRAFARDLAWQSPKPAHVSAAGAGIGDDGRVILTQNPDTAVHVVELPLSGDT
                     LSAVRLDTAPQSGFGNFVLTGLKAEVVPPDTQAAPRARFVRVELPGEKKMLQLAEVQV
                     FSGAENLALKGEARQSSTYADAEAARAVDGNTNGEYNNGSVSHTNDNDKDTWWEVDLK
                     AARPVDRVVVWNRLDGTGKRLDGFRLVLLDEKRQTVWKSDPAPAPDLEKSFAISGPVP
                     VVFTTALADYEQAGFEASKFLAPKSKGWAVGGAQDKPHTLTLLTKTPVAVPPGAKLRV
                     TLEQRSEHKKHTLGSFRLSFTGDARVRQAAQVPANVAAVLALEQRAPEQQKAVIDHYA
                     RNVAKESAAERARLAAAKKELAAIKPVTVPVMRDLDAKQRRVTKIQLRGNWQDLGAEV
                     SEATPAAFHPLPDGAPRNRLTMAKWLVSRDNPLTARVTVNRLWEGIFGTGIVRSSEEF
                     GSQGELPFHPELLDWLAAELMDSGWDIQHMLRLMLNSEAYRQSSKSSPALNERDPDNR
                     LLARGPRFRPTGEMLRDQALAVSGLLSEKMFGPPVRPMMPNLGLSTAFGRSNDWTVSE
                     GEDKHRRSIYTEVRRNAPYASFTTFDAGNREVCMIRRGRTNTPLQAFVTLNDPVFLET
                     HQAMARRLVREAQTLPERLSRLFMLCLAREPRAEESAALTRLHAESLAMHQSDPAAAA
                     KLATDPLGPAEAGADIPELAVWTTIANVVMNLDEFVMRN"
     gene            complement(12264..15992)
                     /locus_tag="PLP58_03560"
     CDS             complement(12264..15992)
                     /locus_tag="PLP58_03560"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF015722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional serine/threonine-protein
                     kinase/formylglycine-generating enzyme family protein"
                     /protein_id="HRJ07632.1"
                     /translation="MSDESPASGDVEEPDRTTRVAPPSPSDLIRANAGRILGDMLDEG
                     LAGMECEGDTVGPYRLCEVIGEGGFGNVWRAEQTEVVRREVALKVIKLGMDTAQVIRR
                     FNQERQALASLKHPHIATMLDAGVGPNGRPYFAMELVRGGTITRWCQEHNATLPERLR
                     LFIQVCQAVQHAHEKGILHRDIKPSNVLVTEVDGAPVPKVIDFGIAKAMSGSQAELSL
                     LTQTGQVIGTPVYMSPEQIEGGRRLDARSDVYALGALLYEILAGEQAFDLSSVGTGGL
                     AAVRHLILETHPERPSTRLRQKTSAAKHGESGEKPILSTLPADLDWITMKALEKDRLR
                     RYQTAADFAEDVQRHLDSLPVLARLPSLSYKAGRWLRRHRRSVALTSLGAVAATAVML
                     AALHLEAEARRPKRITLAPGELHTNSLGMKFTPVPGTDVLMCIHETRNRDFAAYTAEV
                     PGAVGLWMTGVATGLDPVVEDRDNHPAMRINWEEADAFCQWLSAKEGRTYRLPTDREW
                     SMAAGIGDQETWGPDTTPKSVFKVPDHYPWEGTWPPPAGAGNYSDETLKAATPSTPSF
                     IKGYHDGFATTAPVMSFRPNKTGIHDMGGNVSEWVAERAEPGSRFRILRGAHWGSHLV
                     EDLRKTMATSARHFRDVTTVVKAPQYGFRCVLEAAPPSSAAVTPVPVARETQAPDSKT
                     GLQPAALPPGKFPDPLPDAEISARSVTNSLGMKFIPVPGSDVLFCVHETRRQDYAVFA
                     AASTGLGSTWLSQTVGDHPIGAGNDHPVVSVSWDDARAFCVWLSQKESRLYRLPTDRE
                     WSIAAGLGPHENPRPDDTPEKLGRAGRTEFPWGALFPPPAGMRAGNYADSANHRAFPI
                     LSSIGGYNDDFVTTAPVMSFQANPFGLHDLGGNVAEWCEDWFNTARKEKTYRGSGWKD
                     DLPENILLASRRSANAASGNYQLGFRCVLDPGGIPPSVEKAAVPAEAKPAPPVQPPHP
                     AAISALQARQQPAFPAAMPPDEAKARAISNTLGMKLVPVPGTSVLFCVHETRRQDYAA
                     FAADTPEANTDWKDHSTQGYTPQGSLDAHPVTRVSWQDAQAFCAWLSRKENRAYRLPT
                     DREWSLAAGIGHLEKWEVWTTPSTVHKPKGHYPWGGATWPPASPVVNYSDETRRKLAP
                     DPTAKYFTGYDDGHAYTAPVMSFPANALGIHDLAGNAAEWCEDAFDESRTTFPTRGGA
                     WSASSADWVQTSFRVPHPYNVRRLSNGFRIVLEQEAKP"
     gene            complement(15989..16735)
                     /locus_tag="PLP58_03565"
     CDS             complement(15989..16735)
                     /locus_tag="PLP58_03565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ07633.1"
                     /translation="MAAPLTDTLFPSTRWSLLHKLREGTEEEARAALETLCRAYWQPL
                     YCVARRRQLSEHDAQDAVQGFFESILRRETFATADEAEGKLRQLLLRAFDNFHTQQWQ
                     KATRQKRGGGVEHVCLDAAMDPARAEQVYQKSDPAQSLEALYNREWASALLSRALEAL
                     RADYEKRGWQERYELLLGPLIQQGDETQLGELAASAGMSAGALRVMLHRMRTHYRGQI
                     ERELSHTLGTSDPATLREEMQELFKAFSTP"
     gene            17073..22679
                     /locus_tag="PLP58_03570"
     CDS             17073..22679
                     /locus_tag="PLP58_03570"
                     /inference="COORDINATES: protein motif:HMM:NF037015.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cadherin-like domain-containing protein"
                     /protein_id="HRJ07634.1"
                     /translation="MMLLAWVACGASAMAGTWSQAPWTGDSTTGLHASSNIWAYRFGS
                     ATTATVNGVTVPGSPFSLTSANVDVTLTSSYQGAVNNLTALGGTGSALLAVDFVYNGA
                     QGPLTATVKNLTVGQDYVVSFFGVGFDADAARNVNFTSGSDSLVVNQNHYGQGNGIRV
                     DYTFTAEAATRGFTLMETAPFYSWGLNAIALSRSASAPTAATGAAGGVFTTTATLAGS
                     INANGADTAVTFQYGLTTSYGSTVTAAQSPVTGETDTAVSAAVSGLTPGTTYHYRVVG
                     QNFQGTTPGADATFTTPATNLGLYAPPANGADVYVADGTNHRIVRYTSSGTLVGDFAT
                     SATGINGPSSLAFDSAGNLYVGNNGDGTVWKYAPNGTGSLFATVPDGVWNMAFDADGN
                     LYVSNFGTAITKISPSGTNLGNVDNFSGSHTCLGVAFDAAGTLWAGDHFAFSINRYPP
                     GQAATRHTQSAVNNPRGLAFDTAGNLFVANIGNTTISKYAFTGGALSTTGSVFATTSG
                     FNPLCLAFDSTGNLYATANNSGVVGKYSSTGSYLGAFATVSGSSLIRGIAFSPLAEAP
                     VVSSPTSTSVTATGATLGGNVTADGGASITERGVVYSATATNSNPAIGGSGVTKVTAP
                     GTTGVFTTGVSGLTTRTSYSFKAYAINGAGTSYSDTGTFSTPNTLPTAPNGNATGTTG
                     DPNTVTISFPATDADGDAVAITGAVSGTGITVDSFTATTVTFTPAASFTGDGSFTYTV
                     GDGFGGTATGTITVAVTDNDAPVAGAPTEGFTLLALTVDAGTGVVDLPDYRSQVVALD
                     NAGVVSLAQAPAPGSVLGQGPVQVSITAEDAAGNVSDALVLTVVVGPAVAPVAGWSFT
                     AWTGDSSTGIHASSNVWAHRFGSAATATVNGVTVPGSPFSLTSADVDVTLTSSYQGAV
                     NNLTALGGTGSALLAQDFVYNGVQGPLTATVKNLTVGQKYAVSFFGVGFDAGVARSVN
                     FTSGADSQVVNQNHYGLGNGIRVDYHFTATAATRSFILMETAPLYSWGLNAITLRKVS
                     VPDLVTDAATDVTTESATLNATVNPDGLAATAHFEYGLTTAYGSTAAITLSPADGSSA
                     QAVSAPVSGLTAGRTYHYRVALTNLHGTFYGDDQTFSTVQIPSLFALSARTISVPQGS
                     ASVTIPLHRTGGSHAAGVQLSTMDGTASAVPPFAAGLAGKDYQALTTTVDFAEGEMQK
                     EVEITLIPRTGTNVPNTRFQVVLTGAGWDDIPADPKLGHPITATVQVLANDTTAPTLT
                     VKTPANGATLNHAPPVVITGTVGDKNGIDRVEIEHGGELIEADLGSATRSTSIPFSGE
                     IYPAADGPVTFTVTAYDLNGNSTVVTRAFNHVRWYDVVVSRSVPGGLATPEAAGMVTL
                     ASTKGSFSTLQPARGSIDQQGSVLPGAPVTLTARPAAGHVFSHWEGLPVDAVMSGAVA
                     SFTMPAEDVTEILAVFVANPFAALGTTAVFHGLLAPHQTQRSNAALGSLNATLTTSTG
                     ALSGKLLLGGVTTSFAGTVQGNGDVWLKNAGGPMTGALEIPGIGTLVLFFASDSLDIS
                     LFGADTDSFGTARAAIHSAANPVPATLLNDAKQGFYTVALPALEQTPSKPASTYPQGA
                     GYGTLTLKTNGTLTLAGVLADGSKFTAASALVSGNTSPVHAQLPTPGSATLKGGSLLG
                     MLAFDPDAPDTDVSSASLGWAVFGGKGGPPPESGSGLLWLRPEVVQQTGTTAAALATQ
                     LYTEGWEQGITVGLVGALYDKAVTVQSALGLPAAGPDGNAVLAFEDGKLLDTVAYTTL
                     NVDGGKVVKLPSTDKRYTLTLTGNTGAMKGAFTPFWSNPGTALPAFNGVILQKGANKG
                     GWGHFISNRQHDLDPQSGSVTLGAQP"
     gene            complement(22761..23777)
                     /locus_tag="PLP58_03575"
     CDS             complement(22761..23777)
                     /locus_tag="PLP58_03575"
                     /EC_number="2.3.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009962358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0033818 -
                     beta-ketoacyl-acyl-carrier-protein synthase III activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase III"
                     /protein_id="HRJ07635.1"
                     /translation="MPPPSQQPFCASSIIGTGSFMPEKVLTNDDISKIVDTSDEWITT
                     RTGIKERRIAAEGQATSDLAAEAARRAIADAGICPEEISLIVVATVTPDMFFPSTACF
                     VQKKIGAANAVCFDISAACSGFLYALQVARHFINTGNRTTALVIGAEKLSSLINWQDR
                     NTCVLFGDGAGAVVIRRAEEGSDAQGRVLSTVMGSDGNLADLLKVPGGGSACPITAEN
                     ALSRPNTIHMEGRETFKHAVTRMCQASEQALEMAGLKKEDITLIIPHQANLRIIQAIA
                     DRLGLPHDKAFVNLDKYGNTSAATIPVALDEARRAGRLKKGDIVLLVAFGGGFTWASS
                     VVRW"
     gene            complement(23764..24783)
                     /gene="plsX"
                     /locus_tag="PLP58_03580"
     CDS             complement(23764..24783)
                     /gene="plsX"
                     /locus_tag="PLP58_03580"
                     /EC_number="2.3.1.274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009962357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043811 - phosphate:acyl-[acyl carrier
                     protein] acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HRJ07636.1"
                     /translation="MKIALDVMGGDHAPQNPMGGVKLALETLPRIEKILLVGVPEVIE
                     REMAAQGIASPKLEIVPALQVVDMSDSGLDAVRKKKDSSISRAVDLVKDKKADAVVSA
                     GHTGAAVTASLIKLRTLPHIERPAIAAIMPSVSHHWVLIDAGANPDSLPEHLVQNALM
                     GSAYAEHVLGRRKPRVGLMSNGTEEEKGNALCKETAKLLRTTPGLNFIGNVEGHDLWH
                     EPPDVVVCDGFTGNIILKTSEALAESLITMIKGEIMASTMTMLGGKLARPAFKRVHKR
                     ISADEAGGMPLLGLNGITIIAHGGASAYAMKNAIRMACDTITQQINPHIEATVSQHFL
                     IHATS"
     gene            complement(24868..26013)
                     /locus_tag="PLP58_03585"
     CDS             complement(24868..26013)
                     /locus_tag="PLP58_03585"
                     /inference="COORDINATES: protein motif:HMM:NF012585.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HRJ07637.1"
                     /translation="MTDFFPENPVAVLCSGGDAPGMNAFLRALVRLGLNRHNVPVLGI
                     KDGYRGLVKAARQAGASPVELQQLKDAIVRNPGRKGMIENQPVVLMDHASVSGIMGKG
                     GTVLGSARCKEFEKREVRAEVIALLGALGVRALVVVGGDGSLAGARCLAEESGLRIIG
                     VPATIDNDLQFTELSLGVDTAVHTLVWAVDHFIDTARSHRRVMVLETMGRESGDLARM
                     AALASGAEMVITPEGGPLDAAQIEKLAHRIEEAMIRGRGHAIVLLAEGVKFDPPQKRN
                     GAYVLRDAFQQYFDRPEGAFSDLEVRPSVLGHLQRGGHATPQDCILAARFAEKAWKSL
                     LDPDCGNGITALRENRVALVPFDCPDMPERASATRAMDDLHEALSFW"
     gene            complement(26108..26653)
                     /gene="ilvN"
                     /locus_tag="PLP58_03590"
     CDS             complement(26108..26653)
                     /gene="ilvN"
                     /locus_tag="PLP58_03590"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009963980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1990610 - acetolactate synthase regulator
                     activity [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HRJ07638.1"
                     /translation="MSDRIPATTDKKPAPQADIINIHTLSVYVNNQPGVLGRICNVFS
                     RRGFNIESLVVSQTRDPRYSRMTIGISGHPEGLHQIIMQVNKLIDVIHCIEHTDRDAV
                     AKELVLVKISTGHDQRTEILQIVEHYGGKTVDLQDDSLIAMITGNTDKLDAAIRLLNK
                     FEIVETVRTGKVVMARGMETT"
     gene            complement(26696..27493)
                     /locus_tag="PLP58_03595"
     CDS             complement(26696..27493)
                     /locus_tag="PLP58_03595"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013704.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HRJ07639.1"
                     /translation="MKANLIIQGDALTELRKLPECAAQAIIADPPYFNVLEDEAWDTQ
                     WKTAADYLAWCEEWAGEAMRVLCDEGLCFIFGQLGKREHTFLHLMSQLSRSHQFHDLI
                     IWDRAVGYNERRDSFTPQYEMILVLRKGDEVKFNKDAVRIPYDEKTIAQYLKDTRYKN
                     MDARKAHLEKGKFATNILRVPSLKGTSKEKCGHPSQKPLDLIEKLVLCSTDEGDLVLD
                     PFFGSGTTAAAALRLKRKWIGIERDETYVQLARRRLHALASAEPQLF"
     gene            complement(27533..29260)
                     /gene="ilvB"
                     /locus_tag="PLP58_03600"
     CDS             complement(27533..29260)
                     /gene="ilvB"
                     /locus_tag="PLP58_03600"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009963977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005948 - acetolactate synthase complex
                     [Evidence IEA];
                     GO_function: GO:0003984 - acetolactate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009099 - valine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biosynthetic-type acetolactate synthase large
                     subunit"
                     /protein_id="HRJ07640.1"
                     /translation="MSTPQMDGAQALIKTLADLGIEYVFGYSGGAALPIFDALETVKT
                     NMKFILVRHEQGAVHMADGYARATGKPAVVLVTSGPGAGNTVTGIMTAQMDSVPMIIL
                     TGQQVTWMLGKDAFQEADIFNITAPVCKHSYLVKETNALPRIAKEAVHIATTGRPGPV
                     LIDIPKDISQSPFTGTFDDPMDLPGYHPEDAQKIDQIAIKEAARLLSQSQRPVILAGQ
                     GAMIARADKELLMLAETLQCPVTTTLLGKGVFPETHPLALGMLGMHGTAYANKAICEA
                     DLIFNVGSRFDDRIIGKPHMFGRNAKIIHIDIDAAEHGKMIRTDVPITGDAKAALSDL
                     LPLVEKLPTGDWLAHLDGYKKKYPLGYKKQGGLRMQQVLDELYHLTQGKAIVTTDVGQ
                     HQMWAAQFYKNDESYHWISSGGAGTMGFGFPAAIGAQFACPNKTVVAVCGDGGFQMTL
                     FELATAAIHKLPVKILVLNNSYLGMVRQWQELFFDNRESGVDLLGNPDFVKLGEAYGI
                     KGWHIRRPADVGRILQQALDYNDGPCIIEAECIKHENVFPMIPAGAALEDMLTEAPKH
                     KMEKPTGST"
     gene            complement(29532..30833)
                     /locus_tag="PLP58_03605"
     CDS             complement(29532..30833)
                     /locus_tag="PLP58_03605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009960473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1501 domain-containing protein"
                     /protein_id="HRJ07641.1"
                     /translation="MKSELLKLDDHSRRQFMMGAASATLGVTLFPGLSAKMQAAEKMI
                     TGPGTPGFGKAKNVIFLWMSGGMSHIDTFDPKTGMTKGFSDPVKAKAEGIDYLGGYLP
                     KLAENANKLSIIRSMTSKTGVHDSGTYIMKTGYEPRGTIVHPLVGVWASHFLGRIKGV
                     TLPDSVVVNSGSAYPGAGFFPPSMAPIPISSPESGLQNIRPTTSDSMFGKRVELMNEF
                     DSAFRKRFDTEDVKSYNEFYDETMKIMKSEDLKAFDLSQESADTRDKYGRSSFGQGCL
                     LARRLVESGVRFVEVRMGGWDHHNTIDTAMTNNGTTLDTGFSALLLDLEAKGLLDSTL
                     VVLGSEFGRTPGINENDGRDHYPLAYSTVFAGGGTKRGFTYGATDKDGRRPADKQTTP
                     QDFVATIGHAMGLPVNEVVMSPSNRPFTVGDKGVPVVDIFA"
     gene            complement(30873..33038)
                     /locus_tag="PLP58_03610"
     CDS             complement(30873..33038)
                     /locus_tag="PLP58_03610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009960472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1549 domain-containing protein"
                     /protein_id="HRJ07642.1"
                     /translation="MKPTRSMIAVLLFAGFGTSSALAEVRTWTDVQGRQVSASLVRIE
                     GEHIVLQTQDGAEHRFELSRLSAEDQAFAKSSAPAAADTAQPQMIANATVAQAAQKIN
                     ELVAKGLVRANPDRVKNGKPPITQFNPMANDEQFVRRVYLDIVGRIPNYDEAMAFIKD
                     GNPKKRADLIDMLLDSEGFKAHMFNYFAEMLRLKDNLDQANLRGTPYINWVKEQVAKN
                     RPYDQMVHEMMTASGKMWHNGAAGYLLRDAGMPLDNLANTLTVFLGTDVACAQCHDHP
                     FADWTQMQFYEMAAFFGATSTRAPYRNETVMMEVEKLVEKAGLDMRRIRNGIQTYMGA
                     NTYVVRDLDKNMMMLPHDYKYKDGKPGDPVSPKFVTWSSGDRSNPAYKQSKAKEEKLR
                     TSFANWMTHPQNPRFAMTIANRMWKRAFGAGINEPVTNIDDPDQSANPELLKHLAEEM
                     KRVKFNLKEFMRIIYNTRAYQSEATTENIVMGEVYYFQGPMLRRMGAEQAWDSYMTLV
                     LGQPDAYKAPLQDLYSKSIDLNLETVDAQTVLMKYDAYRRMAQKERELMGGGLEMAGD
                     DSMMMDAAPKGAEAATTAQMDGPGKILSYEGMRLLRASEIVQPAPGGHFLADFGQSPR
                     DLIDGSTSIGNVPQVLMMMNGKAQQMLTNRDSLVFRTMDKVKDPSEKVERMFMTIMNR
                     RPTLQEKDIAKRELSASGEEAYSNMIWALINTREFIFVQ"
     gene            complement(33193..34191)
                     /gene="purM"
                     /locus_tag="PLP58_03615"
     CDS             complement(33193..34191)
                     /gene="purM"
                     /locus_tag="PLP58_03615"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009960563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004641 -
                     phosphoribosylformylglycinamidine cyclo-ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HRJ07643.1"
                     /translation="MSKHTYKQAGVDIHEAAGFVDDIGPLVKATQKKRKLANAFGLFA
                     AGYDLSKYKQPVIFTGCDGVGTKLELLLKHDLLENAGKDLVAMNVNDVLTTGADPVLF
                     LDYIGVNKIVKRQLSRIIAGISEYLQDCDCILAGGETAEMPGAVMEGMVELSGFAIGA
                     AEKKHVLNPAEVKKGDVLVGWPSAGFHANGFSLVRRVLEKEKIQFGSKDAASLLAPTL
                     LYHKQCRALKKAGIKPAAMAHITGGGLIENLGRIFTRRGFGCHLQVPYWKNDMAQKIL
                     RHADPKDAWNTFNMGIGWVAILDAKHVRKALKIGKGAVVLGVMDKSGVVTCEALPE"
     gene            complement(34276..35214)
                     /locus_tag="PLP58_03620"
     CDS             complement(34276..35214)
                     /locus_tag="PLP58_03620"
                     /inference="COORDINATES: protein motif:HMM:NF014066.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraB/GumN family protein"
                     /protein_id="HRJ07644.1"
                     /translation="MHFLRRLLCLTVLAAVCACGRDAGQAESVQGGHPVEAGPIGSVW
                     VVDSQDTGGRLYLCGTIHILRKEDYPLAPAYEAAYSRSDKLVFELPPDAAKAGAAARM
                     TQLGTLPPGATLEAEVGSESWSGVKKWCASRGVPPFSMARMRPWLASLMITNNEYAVL
                     GASPDMGVDQHFEKRARRDGKPGEGLETLEFQIQLFAGLSEELQRVMLDQTLAEVGTL
                     PEEYDKMIEAWKHGRLDDLQSMLFREAAKHPELMEIFLAARNRAWMARLEDLLKKGER
                     AMVLVGTGHFTAETGLLHLLKERGYLVRHYREMKED"
     gene            complement(35281..36444)
                     /gene="carA"
                     /locus_tag="PLP58_03625"
     CDS             complement(35281..36444)
                     /gene="carA"
                     /locus_tag="PLP58_03625"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009959629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005951 - carbamoyl-phosphate synthase
                     complex [Evidence IEA];
                     GO_function: GO:0004088 - carbamoyl-phosphate synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HRJ07645.1"
                     /translation="MKAILALEDGRYFEGESFGAPGSRTGEACFNTSMSGYQEVLTDP
                     SYRGQIVAMTYPMIGNYGVNNLDPESSQPHVRGFVIEELCGVPSNWRSTGTLDDYLRQ
                     WDIPGIQGVDTRALTRHLRTRGAMRAVISSEIGSVEEAVRVAAESPPMSGSDFVKEVS
                     TGAAYTWDPDDMESRVWDIPSPSQNREAGPEGAFHPLPEARHHVVAFDFGIKRNILRG
                     LRQSGFRVTVVPSTTSAEEVLARNPDGVFLSNGPGDPAALDYIHKEVSALIGKKPLFA
                     ICLGHQILGHAYGGRTFKLKFGHRGGNQPVKDLRTGKVAITSQNHGFAIDPSSLPSNV
                     EVTHINLNDGTVEGMRHREAPVLSVQYHPEAAPGPNDAKYFFAEFAKMIETGV"
     gene            36642..36962
                     /locus_tag="PLP58_03630"
     CDS             36642..36962
                     /locus_tag="PLP58_03630"
                     /inference="COORDINATES: protein motif:HMM:NF015513.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HRJ07646.1"
                     /translation="MDRKLINREILQSFWKIHILHHASEGGVIGQWMLKELAHHGYAV
                     SPGTLYPLLKRMEGHGWLKSETNPARRGPKAPRTYLITAEGKKVLKVVRQQLRELGVE
                     VGGD"
     gene            complement(36973..38766)
                     /locus_tag="PLP58_03635"
     CDS             complement(36973..38766)
                     /locus_tag="PLP58_03635"
                     /inference="COORDINATES: protein motif:HMM:NF022853.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HRJ07647.1"
                     /translation="MTKLPFFPALAGLLLCASAIHAQIDFGAASAKAKVTAGLVAEVT
                     QAAPGAPFKVAVRLVHEEGWHTYGKELPPEVIGKPTRLIWTLPDGWKVEDMPWPATHE
                     VPSTDGKTSEGYDGTVHLPARIIPAGRAGDTGEIVVKVDALACDPQNCMPAKAEARLS
                     ITLADKVVLNEAAGELFARSMAPAAENKTTVAPARSFGVLLVFAFIGGLILNVMPCVF
                     PVLGIKIMGVVQQAGEDRRRVLLHGLAYTFGILLCFWALGSLVVVLGKSWGFQLQSAG
                     FNLAMCAFFLVFALNMAGVFEIGASAVGVGADLQARQGLGGSFFSGLLATVVATPCSA
                     PFLGPALGFAVTLPVLEALLMFTFIGLGLASPFLLLSLMPGLVSALPRPGAWMESFKQ
                     GMSFLLFGTAAYFVWVLAGMIEGQPLLFTLLGLVVVALGCWIYGRWSPPHKPARTRRT
                     AVVLALAAVAGGLFLGWPQVEKGPVTSGASTENGLTWEAWSPELVDTLRAEGRAVYVD
                     YTARWCLTCQVNKRVYKDAGLQRFIAEKKVALLKADWTNEDPRITEALAALGKAAVPV
                     NVLYIPGQTDPHVLPELLTVENVSDAFGRIP"
     gene            complement(38848..>40242)
                     /locus_tag="PLP58_03640"
     CDS             complement(38848..>40242)
                     /locus_tag="PLP58_03640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009961660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1501 domain-containing protein"
                     /protein_id="HRJ07648.1"
                     /translation="RREFLSRAGGGFGAVALAALMNEPVLGAPSAGAATSAKIPSRFG
                     KAKSVIFCFMEGGPSHLDTFDRKPLLNQIHGQKLPPSFKVPVLAMGESDAPLLESKRA
                     WKRHGQSGLWISDWFENVARHADDLAVIHSCVSDGINHAGGVCQMNTGSIFGGRPSLG
                     AWVNYGLGSENQDLPGFVVIKDSEGTVVNGVRNWGSGFMPAVHQGVEFSGEGVPIKYL
                     DNPKGHTQQRQRDKLDLLAALNTDYNATRADNTELEARIRSYELAYTMQAAAPVAVDL
                     DKESAATKALYGMDQPETAVYGRNCLLARRLVERGVRFVQLYSGAGSKWDSHSRLEEN
                     HSRLCRAVDKPIAGLLSDLKQRGLLDETLVIWGGEFGRTPMSEQGGGRDHNPNGFTMW
                     MAGGGVKGGQTIGATDELGLYAIEDRMHVHDIHSTILHLLGVDHTQLVYHHKGRPERI
                     DQNEGHANPKLAGV"
BASE COUNT         7316 a        12757 c        12593 g         7576 t
ORIGIN      
        1 gggtccggct cgggcggcgg cggccccgcg tccggctggg agaggatggt ggcaaactcg
       61 aagtcaatga ggcagaagcg gccgtcgctg gcgcggtagg tcacattgcg cagataggga
      121 tcctcatggc gcacgccgta ctgctccagt tcggcgtaaa tctggcgcag cttgtcctcc
      181 gtcatgcgct ccacgcgctg gccgcagttg gaggtgacca tgtagaggcg ctccgggtcg
      241 gtgtccagca cgcgcggcac gaaggggcag cccattttct ccaggtagcg cagcacgcgc
      301 acctcgtgct cgaagcgctc cctggccagg tggcctttga aggtcttgtg gacgcgcccg
      361 tcatagccga ggcgcacggt ggcgcgcgcg gtgtctttgt ttgtctgcat gtctcggcca
      421 tgaaggaggc gaacccccgc cggggcaagt cacaaggttg cctttgaccg ggaccggcgg
      481 cgcgctccgc gcgcggggcg cggcggattg gcgcgtcagg ggcggggctt caactcccac
      541 tcgatctcgc gcctgtcctc gcggttgcgc gccttgatct tgtggtcgcg cttggcgcgg
      601 cgctccaggg tcatctggcg ctttttcctg cgcctgcgca gctcctggat ctcgtcctcc
      661 agtttcagga aagccttgta acgctccggt gaaagctccc cgccctccac cgccgcgcgg
      721 atggcgcagc cagcatccct gccgtggccg cagtcatgat gccggcagcg cgcggcgatc
      781 tggtcaatgt cggcgaagcg cccgcggaga gtttcctcgt ccgtccacat ctggacctcg
      841 cggatgcccg ggttgtccac caggatgccg ccgcgcggca gcaccagcag gtcgcgcgcc
      901 gtggtggtgt gccgtccctt gccggtcacc tcgttgacct ccccggtcca cagccattcc
      961 tcgccgatca agtggttgat caaggccgac ttgccaacgc cgctcgaacc cgtgaacgcg
     1021 agcgtggccc cttttttgag gtgctgcttc aaagtcttga aacccttgtt ctcaagcacg
     1081 ctggtgaggc agacttcgac cccgccgccc aggtccctga cagccccgat gacctccgcg
     1141 cagcgctccg cgggatgcag gtctgatttg ttgacgacca ccatggcctc cgcgccgccg
     1201 cgcgcgatca gcgccaggta acgctccagc ctgcgcggat tgaaatccgt tcccgggtcg
     1261 gtaaccacga ccacgcggct ggcgtttgcc acgatgacct gctcctcgga cgagtttcca
     1321 ggggctttcc tcgaaaacct gctctgcctg tgaagccgcg cgcggatcac gttttcatcg
     1381 ccgcttttat caagcgccac ccagtctccc actgcgggca gttccgcgtc cgtctccgcc
     1441 tcgtgataaa ctttccccgc gagcaccgcg tcgcattcct cgatccgtcc gtcttccacg
     1501 ccaagcaggc ggcaggatat tttattgtcg ctggaaacgc gcgcgggcgt ccagccgcgc
     1561 tccagatagg gctcgaattc cgcggcgaat gtttcgttcc atcccaggtg ctgcaaagtc
     1621 attcaactta ccttagcctc catcatggtg gcatcaagcc ttcacaagga ataacatttg
     1681 acgtgtcatg cgcgccactt agtctggcgt ccttgatgaa agcaaacatc cccacccttg
     1741 aacaactgaa acgcgcggcg gccatcgccg agcagatcac ccgcctcgaa gacgaactcc
     1801 aggccatctt cggcaatgcc aagcccggca ggaaaaagaa gtccgccctg gacgcggcgc
     1861 ccgccgccgc cccggtcgcc gcgaaacggg gccccaaggc gaaaaaagcc aagcgcgtca
     1921 ttaacttctc ccccgaggcc cgcgccagga tcgccgccgc tcagaaggcc cgctgggcgc
     1981 gtttccgcaa ggacaagaaa gccgagggct gaacttagct catccttcaa gctcactcat
     2041 gcacggcacc ggacaaccgc gtgccgtgtt tcttttttcc ccgtcaagtc caatccagca
     2101 gccagagaag accgttatgt tccaaatcca gcgcgccgac tttcatgtcc tgccgatgcg
     2161 aacccgtttc cctttcaaat acgggatcgc cagcatgagc gccctgccgc acttgttcgt
     2221 ctcggtcggc atgcgggtgg atggcaggca ggtgaccggc ctgtccagcg agggtttgcc
     2281 gccgaaatgg ttcaccaagg atccttcgac aacctttgag cacgacctcg cggaaatgct
     2341 ctcggtgatt caaaacgcgg cgcgagtcgc cggaaacgcc gctgaaaaac cggtccgctt
     2401 ctttccctgg tggaaggaaa cctctgagga gcagaagcgc tgggctggcc tgcgcaggct
     2461 gcccgcgctg ctttccaacc tcggagtcag cctcatcgag cgcgccgtgc ttgacggatt
     2521 gtgcaaatcg ggagcggtcc ccctgcatgt ctttctaaag tcggacgagg ctggcattga
     2581 tctcggcggg gtcaggccgg agctacacgg catgcgggtg gccggatgcc tgcccgagag
     2641 ccctttgtca aacgtcgccg cccgccacac cgtcggtttg ggtgatccgc tgcggacttc
     2701 cgaaataccc ggtgacgaaa gactggatga cggcctgccc caggccctgg atgattgcat
     2761 ccgaagttac ggactccgct ggttcaaaat caaaatctgc ggcagggagg aaacagacat
     2821 cccgcgcctg cgcgagatca cccgcgtgct tgcggaaggc tgcccgagcg gctttcaagc
     2881 caccctggac ggcaacgagc aatttcagag cctggcggac tttcgcgagt tttacaggct
     2941 gctgcacgcc gacaaggccc tcgcgcccct ctttgacagc cttgtcttca tcgagcagcc
     3001 cctgcaccgc gaccacgcct tgcgcgatga tgtggcgtcc gtgctggagg catggaaaga
     3061 cggccccggc atcatcattg acgaggccga cggctcgctc gacgacctgc cgcgcgcgct
     3121 ggacctgggt tactccggca ccagccacaa aaactgcaag ggcatcgtca aaggactggc
     3181 caattcggcg ctgctgcaaa agcgcgcggc cttcatgacc cgcgcgccca tcctcagcgc
     3241 cgaggacctc gccaacgtcg gccccgtggc gctgctgcag gatctggcca tggtggctgc
     3301 gctcggcatc ccgcatgtgg agaggaacgg ccatcattac ttccgcggcc tcggcatgca
     3361 ctcgcccggg gtccaggagg aggtgctgcg cgcgcacgcg gggctttacc gccgccacga
     3421 gcgcggcttc gcagcgctgg acatccggga cgggctgctg gatctggcga gtgtcaactc
     3481 ggcgcccttt ggctgcgcca tccagcttga tcccgcacgc ttcacgccgc tcgatgcatg
     3541 ggtcaagggc ggcggcctgg cggagctggg atgactcacg cctgcaaagc ggcgagcagc
     3601 ttgtcgtgaa tgccgtcgaa tccgccgttg ctgaaaacga tcaccacatc gccggggcgg
     3661 gtctcctgct tcaagcgcgc cacgatcgcc gccgtgtccg gctcatggaa acatgcgcgg
     3721 cccgcggagg tgacactggc cgccaccttg tccggatcca gccgcagttc ggcgggcacc
     3781 ttctccggat tcgccacgga ggcgaccacc gagccgtccg cctcgcgcag tgcggcgatc
     3841 agctcgttct gcaaaagatt gcgccggctg gtgtttgagc gcggttcaaa cacggcccac
     3901 aaacgcctgc cgggatactt ccgcctcatg ccgcgcaggg tctcgcggat ggcggtgggg
     3961 tgatggccga agtcgtccac caccgtgact ccgccagcct cgccgcgcac ctgctggcgg
     4021 cggcggatgc ctttgaagga ggcaaggccg gcgcggattt cctcgtcgct caagccggca
     4081 tgacgggcgg cgcacacggc catcgcggcg ttgcgcgcgt tgaactcgcc gatcatcggc
     4141 agggagaacg cggcgccgtt gagggtgaag tccgtgcttt ccgggccgcc gccggtgatg
     4201 accagcctct cggagtagtc agggccgagg ccgactgtcc tcacgggcgc ggggcttttc
     4261 agggcgaggc agttcgcgtc gtcgccgttg aggaggacga ggccgttgcg cggcacgctg
     4321 ttgagcaggc gctgaaaagt gagcaggatg tcgtcgaggt cgcggaagat gtccgcgtgg
     4381 tcgaactcga tgttgttcac gatcacgcac tccggcaggt aatgcaggaa cttcgagcgc
     4441 ttgtcgaaat aggcggtgtc atactcgtcg ccctcaatga cgaaaaactc gctgtcattg
     4501 aagcgcgccc cgctctcgaa attggcgggc acgccgccga tgaggaagcc cggatttctg
     4561 cccgcgtgct caagcagcca tgccagcagc gtggtggtgg tcgtcttgcc gtgcgtgccg
     4621 ctgaccacaa agctgcgcct gccctggatc acctcgcgct tgatgagctc cgccatggaa
     4681 atgtagggca gcctgcgctc cagcacggtc tccagctcct cgttcccacg cgagatggcg
     4741 ttgcccacca catgcaggtc cgcgccagcg ggcaggttct ccgccttgta gccctccgaa
     4801 agcgtgatgc ccgccgcgcg cagcacatcg gacatgggcg gatacacttt ttcatccgag
     4861 ccggtgatga tgtgcccctg cgcgcgcaag gccgcggccg tggagcccat ggcggtgccg
     4921 cagatgccga tgaaatgaat gtgcttggga gtcatgaagg gcgcgccggg gcgcgaagcc
     4981 cgccattcaa gccagaatca gcggcaggca aacatgaatt ccatccgggc ggctgcggaa
     5041 ttcaaaggca gcccttcacc gcctccagca ggtcctcgta ccgctcaaag gcccggtatt
     5101 gcgggaaccg ctcctggatg gactccggcg cgtggaaaaa gaacccggtg tcggcctcgc
     5161 ccagcattgt cgtgtcgttg aaggagtcac cggcggcgac gacctggtaa ttgagcgatt
     5221 tgaacgccgc cacggccttc tgcttctggt tgggctggcg cagcctgtag ttcacgatgc
     5281 gcccccgcgc gtcggtctcc agatggtggc agaacagcgc cggccaggcg agctggcgca
     5341 tcaatggcct ggcaaactcc tcgaacgtgt cgctgagtat caccacctgc gtcagcgcgc
     5401 gcagctcgtc gaggaagtcc ctcgccccgt cgagcggcgc cagggtgccg atcacctcct
     5461 ggatgtccgg cagcgccagc ccgtgctcgt ccaggatggc cagccgcccg cgcatgagca
     5521 catcgtagtc gggctcgtcg cgcgtggtgc ggcgcagcgc gtcaatgccg gtcttctccg
     5581 cgaaggcgat ccagatttca ggcacgagca cgccttcgag gtcgagagtg acaagggttt
     5641 gttttttcat gggcgcgtct ccttgtcatg acatggcgcg tctgtccagc atgggagttc
     5701 agcctccggt gggcctgagt gcggccggat tgccgcttgt cccgcccgcc gccgggagta
     5761 tgacaagcgc atgaccgccg gacgcgcacg aagccgcatg ctgggggcgc tggccgcgct
     5821 tctcgtcatg ccatgccctg ccttcgcgca gacgcagccc gcgccgcagt cgctgccttg
     5881 cacgcaaaac ttcggctccg ccaccttcac caccgcgccg gctggcatgg cggcctggaa
     5941 cggactcaac ggcggcggca tcaccagcgc ggcacaagcg gcgaacagcg cgccccaagg
     6001 cgatgccgcc atcagcgcgg ccaccgccgc gcagacgacc ggcggctgtt acggcctcat
     6061 gaccggcggc aacgcgcgct tttacatcca gaccagcagc aacgccacca acggcgtcaa
     6121 ccagctcgcg ctcgccctcg acaccaccgg ctggacgaat gtcgcgctct cctatgacgt
     6181 ggagatcatc aacgccaagc ccaggaccat cggcgtgctc tgccaatacc gcgtgggaac
     6241 cagcggcgct tggaccagcc tcgccgccgc cagcggcagc aatccctaca gccaggccgg
     6301 aggcacggca ggagtgaaga ccagcccgca gatcacactg ccgccgccgg cggaaaacca
     6361 gcccgtggtg caaatccgct gggccgtgtg gcgcggcacg gagaccggcg acagctccgg
     6421 cgcggccatt gacaacattt ccgtctctgg cagcgcggcg ggcgccgtgc tctccgcatc
     6481 ggtcgtcccg ggcatggtcg cggagtcgga tggagccggc gccgcgcagc tcaccgtcag
     6541 ccgcaccggt tccacggacg cgccgctcgc cgtcaccctc gccatcagcg acacgggcgc
     6601 gctcgcctac gacgggccga acccctttcc catccccgcc gggcagagca gcgccaccgt
     6661 gccactgcgc gcggtggaca atgacgtgct cgatggagac aagaccgtca ccctcaccgt
     6721 caccgccccc gcctccgtgg cggcgcaggc cacgatccag gtgctcgatg acgaggacgc
     6781 cttttcgccg ccgccgggtt actatgcgga cgccgtcggg ctctccggca gcgccttgaa
     6841 agccgcgctg aaaatcatct cctcgcccgc cacctacaac agctacgcct attccgacac
     6901 cttcaatccg ctgcgctcgc tcgaccagga tcccgccagc agcggcaacg tcgtcaccat
     6961 ctactccggc gcttcgctcg gcaaaaacgc cgtctatttc cccggcgggc ctgacgcgga
     7021 ctccagttgg agccgcgagc acatctggcc cgactcctac ggcctggacc ctgacaacgc
     7081 gaaccccggc gctcccgacg gcggagacgc cggccccgac ttcaccgacc ttttcaacct
     7141 ccgcccctgc gtccacagcg tgaacgtcct gcgcggcaac cgctactttg acatcaccac
     7201 cggcacgccc tccatccccg cgctggcgcc tctgtgcagc tatgacagca actcctggca
     7261 gccgcgtccc gaggagcgcg gcgacatcgc ccgcgccatg ttttacatgg ccgcgcgtta
     7321 tgacggcggc gatcccctca cgctcgatct cgaactttcc aacacaccga acgccgccgc
     7381 cgggcgcttc ggcaggcttt ccacactcct gcgctggcat gaggaggacc ccgtgagcct
     7441 tgaggaaagg cagcgcaacc accgcattta caccacttac cagcgcaacc ggaacccctt
     7501 cgtggaccgg cccggctttg ccgccgaagt ctggggccac atccgcatgg acaaaaccgc
     7561 cgccacagtc accgaaggcc agcccggcac ggatgccgtg ctcagcctgg cagccgctcc
     7621 cgctgcggat gtcaccctat ccttccagac ctcccccgcc ggacaagtca gcgtctcgcc
     7681 gccgagcctg accttcactc cggcgaactg ggaccagccg caaagcctct ccatcagcgc
     7741 ggtggatgac agccttttcg aggcggagat gactgtcacc atgagcaccg cgatcaccac
     7801 cgccgacacg cgctacgcgg cgctcgaagt gccgcccgtc tccgtcactg tcatcagtga
     7861 cgatcccctc atccagcccg ccgctttgcc cctcagccac ggcggcccct ggagtcccct
     7921 gccctccggc ttccttggcg aggggatcgg cgcgccttac ggcagcagcc tcggcggcga
     7981 caccgccgcc ggctccgcgc gcttcgacac cacgggcgcg cgccttgtca tcggcttcaa
     8041 cgcgcccgcc gaaaccctca cctatcatct gcgcggcaac ccggccgatg gctctgccac
     8101 cagcggcagc ttccacatcc gccactcagc cgacggcctg agcttcacca gcctgcgtgt
     8161 cgtcaccgac aaaaacaaca ccgaccagct tttcaccgac tccgtgccgc gctccgcacg
     8221 ctacctggag ttcatttaca gccagaaaaa ctccggcaac atccagctcg acaagctcgg
     8281 catcaccgcc gcgccctcct tcatctcatg ggccgccgcg cgcgacctct caggagtcaa
     8341 tgccggcatg ctcgtggatg ccgaactcga cggcttcacc aacctcgcag agtacgccct
     8401 cggcggccac ccgcttcagc acgacgccgc cgccatcacg cctgccgcat cacgcgccgg
     8461 cgggcatctg cgcatcaccg ccgtcatccg cgcggatgac ccggacctcg gcgtccaggc
     8521 cgtcaccacc gccgacctcg gaatgccgca aagctggacc gccaccggag tcgtgcaaat
     8581 ccagcccgtg gaccaaagcg gtgtgcctcc cggatttgag cgcgtcactt tccaaagtcc
     8641 cctcaccggc ggccgcgctt tcatgcggct tgtcatcagc ctccagggcg ggggctgagc
     8701 ggagacggat gcggcaaaaa acatcccgtt ccaaaacgta ttgcctcacg gatcatgact
     8761 gtttttcacc gccttctcat cagcactgca tgcctcggcg ccgcctccgg cgcggaggtg
     8821 gattttgccc ggcaaatccg gcccattctc tcggagaact gcttcttctg ccacggcccc
     8881 gatgacaaga aacgggaggc ggacctccgt cttgacgacg aggcggcagc caagaaaaac
     8941 aacggcggag tcatcgccgt ggcaccgggc gatccggaga aaagcgccct catccagcga
     9001 atcaccagca ccgaccctga cgagatcatg ccgccgccga agcagcacaa gacgatctcg
     9061 cccgcgcagg tggcgctcct gaaggagtgg atcaagcagg gcgcgaaatg gggcaagcac
     9121 tggagttatg agaaggtggt gaggcccaag gttcccgcag gcgcaaagaa cccggtggat
     9181 gcgtttctgg cggcacgcct gaaaaaggaa gggctgtcgt tttctcctgc ggcggaccgg
     9241 gccacgctca tacggcgcat ggccatggac ctcaccggcc tgcctccgac actcgaagaa
     9301 ctggcgcgct tttcctccgg cacttcaaac ccgtccgact ggtccgggcg cgtgatctcc
     9361 cactacctct ccaagcccgc ccacggcgag cactgggccg cgcagtggct cgatctggcg
     9421 cgctacgccg actcctccgg ctatcccagc gaccagccgc gtgaaatctg ggcctatcgt
     9481 gactgggtga tccgcgcgtt gaacgtgaac atgccgttcg accagttcac catcgagcag
     9541 ctcgcgggcg atctgctgcc cagcccgtcg gacgaccagc tcatcgccac cgcgttccac
     9601 cggaacacga tgacacaaaa cgaaggcggc acgaatgacg aggagtttcg caacgcggcg
     9661 gtcattgacc gcgtcaacac caccttcgcc gtgtggatgg gcaccacgat ggcctgcgcg
     9721 caatgccaca cgcacaagta cgaccccatc accatcgagg agtacttcca gttccacgcc
     9781 ttcctcaatc aaagcgccga cagtgacaaa aaagacgagg tgccggtgca cagcttcgac
     9841 acgccggaga tcaaaaagca gcgccggacg ctccaggccg agatcgcggc gctggagtcg
     9901 aaattctcgc agccgccccc ggaatggctg gcgggtctcg acgcctggga ccgcgccttc
     9961 gctcgtgatt tggcgtggca aagcccgaaa cccgcgcatg tgagcgcggc cggcgcgggg
    10021 atcggtgacg acggtcgtgt cattctcacc caaaaccccg acactgccgt gcatgtcgtc
    10081 gaactgccgc tcagcggtga cacactcagc gcggtgagac tcgacacggc gccgcaatcc
    10141 ggcttcggca attttgtgct caccggcctc aaggccgagg tggtgccgcc ggacacgcag
    10201 gcagcgccgc gggcgcgctt tgtccgcgtg gaactgcccg gcgagaaaaa aatgctgcaa
    10261 ctggcggagg tgcaggtctt cagcggcgcg gaaaacctgg ctctgaaagg cgaggcgagg
    10321 caaagcagca cctacgctga cgcggaggcg gcgcgcgccg tggacggcaa cacgaacggc
    10381 gagtacaaca acggcagcgt ctcgcacacc aacgacaacg acaaggacac atggtgggag
    10441 gtggatttga aggcggcgcg acccgtggac cgcgtggtgg tgtggaaccg tctcgacggc
    10501 accggcaagc gtcttgacgg cttccgcctc gtgctgctcg atgagaaacg ccaaactgtg
    10561 tggaaaagtg atccggcgcc ggcgcccgac cttgagaagt cattcgcgat cagcggcccc
    10621 gtgccggtgg ttttcacaac ggcgctggcc gactacgagc aggctggatt tgaggcgtcg
    10681 aaattcctcg ccccaaaaag caagggctgg gccgtcggcg gcgcgcagga caaaccgcac
    10741 acgctcactc ttttgacgaa aacgcccgtc gccgtcccgc cgggtgcgaa gctgcgcgtg
    10801 accctggagc agcggagcga gcacaagaag cacaccctcg gcagcttccg tctcagcttc
    10861 accggcgacg cgcgcgtgcg gcaggccgcg caagtgccgg cgaacgtcgc cgccgtgctg
    10921 gcgctggaac aacgcgcgcc ggagcagcaa aaggccgtca ttgaccacta tgcgcgcaac
    10981 gtggcgaagg agtccgccgc cgagcgcgcg cgcctcgcgg ccgcgaaaaa ggaactggca
    11041 gccatcaagc cggtgactgt gcctgtcatg cgcgacctcg acgcaaagca gcgccgcgtg
    11101 acaaaaatcc agcttcgcgg caactggcag gatcttggcg cggaagtcag cgaggccacg
    11161 ccggccgcgt ttcatcccct gccggacggc gcgccaagga accgcctcac catggcaaag
    11221 tggctcgtca gccgggacaa tccgctgacc gcccgcgtga cggtgaaccg cctgtgggag
    11281 ggcatcttcg gcaccggcat cgtgcgcagc agcgaggagt tcggcagcca gggcgagctg
    11341 ccatttcatc ccgaactgct cgactggctc gccgccgagt tgatggacag cggctgggac
    11401 atccagcaca tgctgcggct catgctgaac agcgaagcct accgccaaag ctcaaaatcc
    11461 agccccgcac tcaacgagcg cgaccccgac aaccgcctgc tggcgcgcgg cccgcgcttc
    11521 cgccccaccg gcgagatgct gcgcgaccag gcgctcgccg tcagcggcct gctatcagaa
    11581 aaaatgttcg gcccgcccgt gcgtccgatg atgccgaacc tcggtctcag cactgccttt
    11641 ggacgcagca atgactggac tgtcagcgag ggcgaggaca agcaccgccg cagcatctac
    11701 acggaggtgc ggcgcaacgc gccctacgcc agcttcacca ccttcgacgc cggcaaccgc
    11761 gaagtctgca tgatccgccg cggccgcacc aacacgccgc ttcaggcctt tgtgacgctg
    11821 aacgaccccg tcttcctcga aacgcatcag gccatggcgc ggcggctcgt ccgcgaggcc
    11881 caaaccctgc ccgaacgcct ctcccgcctc ttcatgctct gcctcgcgcg cgagccgcgc
    11941 gcggaagagt cagccgcatt gacccggctg cacgccgaaa gccttgcgat gcatcaaagc
    12001 gaccccgccg ccgccgcaaa gctggccacc gaccccctcg gccccgccga agccggcgcc
    12061 gacatccccg aacttgccgt ctggaccacc atcgcaaacg tggtcatgaa cctggacgag
    12121 tttgtcatgc gcaattaacc gattgcgaac ccagcccgca aaaccccgcg attcaaattc
    12181 gccaaacctt ctccggcatt acgccaagct gcgttcgtgt catgcgagga tgagatcgga
    12241 aaatgatgga gactgcgaag cgctcatggc tttgcctcct gctccagcac aatgcggaag
    12301 ccgttggaca ggcggcggac gttgtatggg tggggcaccc ggaaagaggt ctggacccag
    12361 tcggcgctgg aggcgctcca ggcgccgccg cgtgtgggga aggtggtgcg gctttcatcg
    12421 aaggcgtcct cgcaccactc cgccgcgttg ccagcgaggt cgtggatgcc gagtgcgttt
    12481 gccgggaaac tcatcaccgg cgcggtgtag gcgtggccgt cgtcatagcc ggtgaagtat
    12541 ttggcggtgg gatcgggggc gagcttgcgg cgggtttcgt cgctgtagtt gacgacgggg
    12601 ctggcgggcg gccaggtggc accgccccag gggtagtggc ccttgggttt gtggacggtg
    12661 ctgggcgtgg tccacacctc ccatttttcc agatggccga tgccggcggc gaggctccac
    12721 tcgcggtcgg tgggcaggcg gtaggcgcgg ttttccttcc ggctgagcca ggcgcaaaag
    12781 gcctgcgcgt cctgccagga gacgcgggtg acggggtggg cgtccagtga gccctgtggc
    12841 gtgtagccct gggtgctgtg gtctttccag tcggtgttcg cctccggggt gtcggcggca
    12901 aaggcggcgt agtcctggcg gcgggtctcg tggacgcaga acaggacgct ggtgccgggc
    12961 acgggcacga gcttcatgcc cagggtgttg ctgatggcgc gcgcctttgc ctcatcgggc
    13021 ggcatggcgg cgggaaaggc gggctgctgc cgggcttgca gggcggagat ggcggcgggg
    13081 tgcggcggct ggactggagg ggcgggcttt gcttcagccg gaacagcggc cttttccacc
    13141 gagggtggaa tgccgcctgg atcaagcacg cagcggaaac caagctggta attgccagag
    13201 gcggcattgg ccgagcggcg tgaggccagg aggatgtttt ccggcaagtc atccttccag
    13261 cctgagccgc gataggtttt ttctttccgg gcggtgttga accagtcttc gcaccattcg
    13321 gcgacgttgc cgcccagatc gtgcagaccg aaagggttcg cctggaaact catcacgggc
    13381 gcggtggtga caaagtcgtc attgtatccg ccgatggatg acagaatggg gaaggcgcga
    13441 tggttggcgc tgtcggcata gttgcccgcg cgcattccgg cgggaggagg aaagagcgcg
    13501 ccccagggaa actcggtgcg gcctgcgcgt ccgagctttt cgggcgtgtc atccggtctc
    13561 ggattctcgt gcggccccag accggcggcg atactccact cccgatcggt gggcaggcgg
    13621 taaaggcggc tttccttctg gctcagccag acgcagaagg cgcgtgcatc gtcccagctc
    13681 acggagacaa cgggatggtc atttcccgcg ccgatggggt ggtcgccgac ggtctggctg
    13741 agccaggtgc tgcccaggcc cgtgctggcg gcggcaaaga cggcgtagtc ctggcggcgc
    13801 gtctcatgga cgcagaagag cacatcactc ccgggcactg ggatgaactt catgccaagg
    13861 ctgttggtga cgctgcgcgc gctgatctcc gcgtcaggaa gcgggtcggg gaatttgccc
    13921 ggaggcagtg ccgcaggttg caggccggtc tttgaatcag gagcttgcgt ttcccgcgcc
    13981 acgggcacgg gtgtcaccgc cgcacttgag ggcggggcgg cttccagcac gcagcggaag
    14041 ccgtattgag gggctttcac gacggtggtg acatctcgga aatggcgcgc ggaggtggcc
    14101 atggtcttgc gcagatcctc caccagatgg ctgccccagt gggcgccgcg caaaatgcga
    14161 aacctgctgc ccggctcggc ccgctccgcc acccattcgc tgacattgcc gcccatgtca
    14221 tggatgcctg tcttgttagg ccgaaagctc atcaccggcg ctgtggtggc gaatccatcg
    14281 tggtagcctt tgatgaagga gggggtggaa ggcgtggcgg ccttgagcgt ctcgtcgctg
    14341 taattgcccg cgcctgctgg tggcggccag gtgccctccc aggggtaatg gtccggcact
    14401 ttgaagaccg acttcggcgt ggtgtccggc ccccaggtct cctggtcacc aatgcccgca
    14461 gccatgctcc actcgcggtc ggtgggcagg cggtaggtgc ggccttcttt ggcgctgagc
    14521 cactggcaaa aggcgtccgc ctcctcccag ttgatgcgca tggcagggtg gttgtcacga
    14581 tcctccacca cggggtcgag gcccgtggcg actcccgtca tccagaggcc gaccgcgccc
    14641 ggcacctcgg cggtataggc ggcgaagtcc ctgttgcgcg tctcgtggat gcacatcagc
    14701 acatccgtgc cgggcacggg ggtgaacttc atgccaaggc tgttcgtgtg cagttcgcca
    14761 ggggccagcg tgatgcgctt tggcctgcgt gcctctgcct ccagatgcaa tgcggcgagc
    14821 atgacggctg tggcagcgac cgctccgagg ctggtcagcg caaccgagcg gcggtgcctg
    14881 cgcagccagc gccctgcctt gtagctgagg ctgggcaggc gggccagcac gggcaggctg
    14941 tccagatggc gctgcacatc ctccgcaaag tccgctgccg tctggtagcg gcgcaggcgg
    15001 tccttctcca gcgccttcat ggtgatccag tccaggtcgg cgggcagcgt ggagaggatg
    15061 ggtttttcgc ctgattcacc gtgcttggca gctgaggtct tctgcctcag ccgcgtgctc
    15121 gggcgttcgg gatgggtttc gagaatgaga tgccgcaccg cagccagacc acctgtgccg
    15181 acgctgctga gatcaaatgc ctgctcgccc gcgagtatct cgtagagcag cgcgcccagc
    15241 gcatacacat cactgcgcgc gtccagcctg cgcccgccct cgatctgctc cggcgacata
    15301 tagaccggcg tgccgatcac ctggccggtc tgcgtcagca gcgacagctc tgcctggctt
    15361 ccgctcatcg ccttggcgat gccgaagtcg atgaccttgg gcaccggcgc gccgtccacc
    15421 tcggtgacga gcacgttgga gggcttgatg tcgcggtgca ggatgccctt ctcatgcgcg
    15481 tgctgcacgg cctggcagac ctggatgaac agccgcagcc gctcaggcag ggtggcattg
    15541 tgctcctggc accagcgggt gatggtgccg ccgcgcacca gctccatggc gaagtagggc
    15601 cgcccgttcg gccccacgcc ggcgtcgagc atggtggcga tgtgcgggtg cttcaggctc
    15661 gccagcgcct ggcgctcctg gttgaagcgc ctgatcacct gggcggtgtc catgccgagc
    15721 ttgatgacct tgagcgccac ctcgcgccgc accacctcgg tctgctccgc gcgccagaca
    15781 ttgccgaagc cgccctcgcc gataacctcg cacaggcggt aaggacccac ggtgtcgccc
    15841 tcgcattcca tgcctgcgag accttcgtcc agcatgtcgc cgaggatgcg cccggcattg
    15901 gcgcggatga ggtcggaggg ggagggcggc gcgacgcggg tcgtcctgtc aggttcctcg
    15961 acatcccccg aagctgggct ttcgtcactc atggggtgga gaaggccttg aaaagctcct
    16021 gcatttcctc gcgcagtgtg gcgggatcgc tggtgccgag cgtgtgggaa agctcccgct
    16081 caatctgccc ccggtagtgg gtgcgcatgc ggtgcagcat gacgcgcagc gcgcccgccg
    16141 acatgcccgc gctggcggcc agctcgccca gttgtgtttc atccccctgc tggatcagcg
    16201 ggccgagcag caactcgtag cgctcctgcc agccgcgctt ttcatagtca gcgcgcaggg
    16261 cttcaagagc gcggctgagc agggcgctgg cccactcccg gttgtagagc gcctccaggc
    16321 tttgcgcggg gtcgctcttt tgataaacct gctccgctcg ggcggggtcc atggcggcat
    16381 ccagacagac atgctccacg ccgccgccgc gtttctggcg ggtggccttc tgccattgct
    16441 gggtgtggaa gttgtcaaaa gcgcgcagca gaagctggcg cagcttgcct tcggcctcgt
    16501 ccgccgtggc aaaggtctcc cggcgcagga tgctctcgaa gaagccctgc acggcgtcct
    16561 gcgcgtcatg ctcgctcaac tgccgccgcc tggccacgca gtagagcggc tgccagtagg
    16621 cgcggcagag agtctccagc gcggcgcgcg cctcttcctc ggtgccctcg cgcagcttgt
    16681 gcaggagcga ccagcgtgtg gagggaaaga gggtgtcggt gagcggagcc gccatgtgtg
    16741 tgccgcgccg cttttctcac cggatgcaaa tattgtcact ttttttctgc cggggcgtaa
    16801 cggcgggcgg aaaacaggac tggagagggt gacgggcatt ccgccctgaa tcagaaacca
    16861 tcgctcccat gaatcccaca tcctccctgc catgcgcgcc gagtttgcat caggcccgaa
    16921 aggcttgttg ccacacaacg cggccccacc atccccccgg agactctcac tctcccaaca
    16981 aagaatccgg gggttcgtcg tcacaagatc cccaacgcgg cgcgcccgga agggttcgcc
    17041 cttcaccagg gcgggccttg tggctgagtg tcatgatgct gctggcctgg gtggcctgcg
    17101 gagcaagcgc gatggctgga acctggagcc aggctccttg gacgggggac tccaccacgg
    17161 gcctccatgc atccagcaat atctgggcat atcgttttgg ttctgccacg acggcgacgg
    17221 tcaacggggt cacggtgcct ggcagccctt tctcactgac cagcgccaat gtggacgtga
    17281 ctctcaccag cagctaccag ggtgcagtga acaatctcac cgcgctcggt ggcacgggca
    17341 gcgcgctcct ggcggtggac ttcgtttaca atggagccca gggtccgctc accgccacgg
    17401 tgaagaacct caccgtcggc caggattatg tcgtttcttt cttcggggtc ggctttgatg
    17461 ctgatgcggc gaggaatgtg aacttcacct ccggctcgga cagcttggtg gtcaatcaaa
    17521 accactacgg ccagggcaat ggcatccggg tggattacac cttcacggca gaggcagcca
    17581 cacgcggctt cactctcatg gagacggcac cgttctattc ctgggggttg aacgcgattg
    17641 ccctgagcag gagcgcgagt gcgccaacgg ctgcgaccgg cgcggcgggc ggtgtgttca
    17701 ccaccactgc gactctcgcc ggcagcatca acgccaacgg ggcggatacg gcagtgactt
    17761 tccaatacgg actgacaacg agttacggct cgacggtgac ggccgcgcag tcaccggtaa
    17821 ccggtgagac tgacacggcg gtgagcgccg ctgtcagcgg cctgacaccg ggcacgacct
    17881 atcattaccg cgtcgtcggg cagaacttcc aaggcacgac ccctggtgct gacgcgactt
    17941 ttaccacacc tgcgacgaac ctgggactat atgccccgcc tgcaaacggg gcggatgtgt
    18001 atgtggcgga tgggacgaat cacagaatcg tccgatacac ttcgtctggg actctggttg
    18061 gcgacttcgc cacttccgcg accggcatca acggtccctc gtccctggcc tttgactcgg
    18121 caggcaatct gtatgtcggc aacaatggcg atggcacggt ctggaaatat gcgcccaatg
    18181 gcactggcag cctctttgcc acggtccccg acggggtttg gaacatggct tttgacgcgg
    18241 atggaaactt gtatgtctcc aacttcggca cagcgatcac gaagatcagc ccgtcaggga
    18301 cgaatctggg caacgtggac aacttcagcg gctcgcacac ttgcctgggc gtggccttcg
    18361 acgcggctgg cacactttgg gcgggcgacc atttcgcatt ttccatcaat cgctatcctc
    18421 cgggccaggc cgcgacgcgc cacacgcaat ccgccgtgaa caatccaaga gggctggctt
    18481 tcgacactgc cggaaacctc tttgtggcca acatcggcaa cacaacgatc tcgaaatatg
    18541 cattcactgg cggcgcgctt tcgacaacgg gctcggtgtt cgccaccacc agcggcttca
    18601 atcctttgtg ccttgcgttc gacagcacgg ggaacctcta cgcgaccgcg aacaacagcg
    18661 gtgtcgttgg aaagtattcc tccacgggaa gttatctcgg tgcttttgcg accgtgtcgg
    18721 gaagctccct gatcaggggg atagcgttca gccccctcgc ggaagcgcct gtggtatcct
    18781 cgcccaccag cacttccgtc accgccaccg gggcaaccct gggcggtaac gtcaccgctg
    18841 acggcggtgc ctcgatcacc gagcgcggtg tggtttattc agcgacggca accaacagca
    18901 atcctgccat cgggggctcg ggcgtgacca aggtgacggc gcctgggacg acgggcgtct
    18961 tcaccaccgg ggtaagcggc ctgaccacgc gcacgtctta cagcttcaaa gcctatgcca
    19021 ttaacggcgc gggaacgagt tactctgaca ccggcacttt ctccacgccg aacaccctgc
    19081 cgaccgcgcc gaatggaaat gccaccggca ccactggcga cccgaacacg gtgacgatca
    19141 gcttcccggc gacggatgcc gatggcgacg cggtggcgat taccggggcg gtttccggca
    19201 ccggcatcac ggtggattcc ttcacggcaa cgacagtcac cttcactccc gccgccagct
    19261 tcaccggcga cggcagcttc acttacacgg tgggtgacgg gttcggcggc acggccacag
    19321 ggacgatcac ggtggcggtg acggacaatg acgcgccggt ggcgggcgcg cccacggaag
    19381 gcttcacttt gctggcgctg acggtggatg ccggcacagg cgtggtggat ttgccggact
    19441 accgcagcca ggtggtggcc ttggacaatg cgggcgtggt gagcctcgcc caggcaccgg
    19501 ctccaggctc cgtcctcggg cagggtccgg tgcaggtgag catcacggcg gaagacgcgg
    19561 cgggcaatgt gtcggacgcc ttggtcctga cagttgtcgt gggtccggcc gtcgctccgg
    19621 tggcaggctg gtccttcacc gcatggacgg gcgactccag cacgggcatt cacgcatcca
    19681 gcaatgtctg ggcgcatcgt tttggttcgg ctgcgacggc gacagtcaac ggagtcaccg
    19741 tgcctggcag ccccttttcg ctgaccagcg ccgatgtgga cgtgactctc accagcagct
    19801 accagggtgc tgtgaacaat ctcaccgcgc tcggcggcac gggcagcgcg cttctggctc
    19861 aggacttcgt gtacaacgga gtccaaggtc cgctcaccgc cacggtgaag aacctcactg
    19921 tcggccagaa gtatgccgtt tctttcttcg gcgtgggttt tgacgccggg gtcgcgcgca
    19981 gtgtgaactt cacctccgga gctgacagcc aggtggtgaa tcaaaaccac tacggcctgg
    20041 gcaatggcat ccgggtggac taccacttca ccgccacggc ggcgacacga agcttcatac
    20101 tcatggagac ggcgcctctt tattcctggg gtctgaatgc catcacgctg cgcaaggtgt
    20161 ccgtccccga tctggtgacg gatgcggcca cggacgtgac cacagagagc gccacgctca
    20221 acgccacggt caatcctgac ggcttggctg ccaccgcgca ctttgagtat ggcttgacca
    20281 ccgcttacgg cagcaccgcc gccatcactc tctcccctgc ggacggctcc tccgcccagg
    20341 ctgtgagcgc gcccgtcagc ggcctcaccg cgggcaggac ttaccactac cgcgtggcgt
    20401 tgacgaacct gcacggcact ttctacgggg acgaccagac cttcagcacg gtgcagatac
    20461 cgtccttgtt cgccctgagc gcaagaacca tcagcgtgcc gcagggcagt gccagtgtga
    20521 cgatcccgct tcaccgcaca ggcggcagcc atgccgcggg tgtccagctt tccaccatgg
    20581 acggcacggc ttccgcagtg ccgccattcg cggcggggct ggcgggcaag gactatcagg
    20641 cgctgaccac cacggtggac ttcgccgagg gggagatgca gaaggaggtg gagatcaccc
    20701 ttattccgcg cacaggcacc aatgtgccga acacacgctt ccaggtggtc ctcaccggtg
    20761 cgggctggga tgacataccg gccgacccca aactcgggca tcccatcacc gccacggtgc
    20821 aggtgctggc gaatgacacc acggctccca ctttgacagt gaagaccccg gccaatgggg
    20881 ccaccctcaa ccatgcgccg ccggtcgtca tcaccggcac ggtgggtgat aaaaacggca
    20941 ttgaccgagt ggagatcgag cacgggggcg aactcatcga agcggatctg ggctcggcca
    21001 cccgctccac gtccattccc ttcagtggcg agatctaccc cgccgctgac ggcccggtca
    21061 ccttcaccgt caccgcctat gacctgaatg ggaacagcac cgtggtgaca cgcgcgttca
    21121 accatgtccg ctggtatgac gtggtggtct cccgctccgt gcccggcggc cttgccacac
    21181 ccgaggcggc tggcatggtg actctggcct ccaccaaagg cagcttctcc acgctgcaac
    21241 ctgccagagg ctcgatagac cagcaaggct ccgtgctgcc cggcgcgcct gtcaccctca
    21301 ccgccaggcc cgccgcaggg catgtcttct cccattggga agggctgcct gtagatgctg
    21361 tcatgagcgg ggccgtggcc tccttcacca tgcctgcgga ggatgtcacg gaaatcctcg
    21421 ccgtctttgt ggcgaacccc ttcgccgcgc tcggcaccac cgccgtgttt cacgggctgc
    21481 tggccccgca tcaaacgcag cgcagcaatg ccgcgctcgg ttccctgaac gccaccctca
    21541 cgacgagcac gggtgctctg agtggcaagc tcctgctcgg cggtgtcacc acatcctttg
    21601 ccggcaccgt gcagggcaat ggcgacgtgt ggctgaaaaa cgccgggggt ccgatgaccg
    21661 gagcgctcga aattcccggc atcggcacgc tggtgctgtt ctttgcctcg gactcgctgg
    21721 acatctccct cttcggggcg gacaccgact ccttcggcac agcccgggcg gcgattcaca
    21781 gcgctgcgaa ccccgtgccc gccacgctgc tcaatgacgc aaagcagggc ttttacaccg
    21841 tggccctgcc cgctctggag cagaccccgt cgaaaccggc ctccacctat ccgcagggcg
    21901 ccggctacgg caccctgact ctgaaaacca atggcacgct caccctcgcc ggcgtgctgg
    21961 cggatggcag caaattcacc gccgcctccg cgctcgtctc cggcaacacc agcccggtcc
    22021 atgcgcaact gcccactccc ggctccgcca cgctgaaagg cggcagcctg ctgggcatgc
    22081 tggcctttga cccggatgcg ccggacacgg atgtcagcag cgccagcctg ggatgggccg
    22141 tgttcggcgg caaaggcggc ccgccgccgg agagtggcag cggcctgctg tggctgcgcc
    22201 ccgaggtggt gcagcaaacc ggcacgaccg ccgccgcgct cgccacgcag ctttacacgg
    22261 aaggctggga gcagggcatc accgtgggcc tcgttggcgc gctgtatgac aaagccgtca
    22321 ctgtgcagag cgcccttggg ctgcccgccg ccgggccgga tggaaatgcc gtgctggcct
    22381 ttgaagatgg caagctcctc gacaccgtgg cctacaccac cctcaacgtg gatggcggca
    22441 aagtggtcaa actgccctcc accgacaagc gctacacgct caccctgacc ggcaacactg
    22501 gcgcgatgaa gggcgccttc actcccttct ggagcaatcc cggaacggcg ctgcccgcct
    22561 tcaacggcgt cattctgcaa aagggcgcca ataagggcgg ctggggccac ttcatcagca
    22621 accgccagca cgatctcgat ccgcagagcg gcagcgtcac cctgggcgcg caaccctgat
    22681 ccgctgtcca gtcacaagac tcgaaacctc aaagccgcag ccttccaggc tgcggctttt
    22741 ttatccgcat gatttgagcc ttaccagcgc acgacggagc tggcccaggt gaagccgccg
    22801 ccgaaggcga cgagaaggac gatgtctccc ttcttcaagc ggcccgcgcg gcgggcctcg
    22861 tcaagggcaa cagggatggt ggctgcgctg gtgttgccgt atttgtcgag gttgacgaag
    22921 gctttgtcat gcggcaggcc gaggcggtcg gcgatggcct ggatgatgcg caggttggcc
    22981 tggtgcggga tgatgagggt gatgtcctct tttttcagcc cggccatttc cagggcctgc
    23041 tcgctggcct ggcacatgcg ggtgacggcg tgcttgaagg tctcgcggcc ttccatgtga
    23101 atggtgttcg ggcggctgag ggcgttttcg gcggtgatgg gacaggcgct gccgccgccg
    23161 gggactttca ataaatcagc gaggttgcca tcgctgccca tgacggtgga tagcacgcgc
    23221 ccctgggcgt cgctgccctc ctcggcgcgg cggatgacga cggctccggc gccgtcgccg
    23281 aagagcacgc aggtgttgcg gtcctgccag ttgatgaggc tgctgagctt ttccgcgccg
    23341 atgacgaggg cggtggtgcg gttgccggtg ttgatgaagt ggcgggccac ctggagggcg
    23401 tagagaaaac cggagcaggc ggcgctgatg tcgaagcaga cagcgttcgc agcgccgatc
    23461 tttttctgca cgaagcaggc ggtggagggg aagaacatgt ccggggtgac ggtggcgacg
    23521 acgatgaggc tgatctcctc cgggcatata ccggcgtcgg cgatggcgcg gcgggcggcc
    23581 tcggcggcga ggtcgctggt ggcctggcct tcggcggcga tgcggcgctc cttgatgccg
    23641 gtgcgggtgg tgatccactc gtcggaggtg tccacgatct ttgagatgtc gtcgttggtg
    23701 aggactttct ccggcatgaa gctgccggtg ccgataatgc tggaggcgca gaagggctgc
    23761 tggctaggag gtggcatgga tgaggaagtg ctgggagacg gtggcctcga tgtgcgggtt
    23821 gatctgctgg gtgatggtgt cgcaggccat gcggatggcg tttttcatgg cataggcgct
    23881 ggcgccgccg tgcgcgatga tggtgatgcc gttgaggccg agcaatggca tgccgccggc
    23941 ctcatcggcg ctgatgcgct tgtggacgcg cttgaaggcg ggcctggcga gcttgccgcc
    24001 gagcatggtc attgtggagg ccatgatctc gcccttgatc atggtgatga gggactcggc
    24061 gagggcctcg gaggttttga ggatgatgtt gccggtgaag ccatcgcaca cgaccacgtc
    24121 gggcggttcg tgccagaggt cgtggccttc aacgttgccg atgaagttga gaccgggcgt
    24181 ggtgcgcagg agcttggcgg tctccttgca gagggcgttg cctttttcct cctcggtgcc
    24241 attggacatg aggcctacgc gcggtttccg gcggcccaaa acgtgctcgg cataggcgct
    24301 gcccatgagg gcgttttgca cgaggtgctc gggcaggctg tcggggttgg ccccggcgtc
    24361 aatgagcacc cagtggtgag agactgaggg catgatggcg gcgatggcgg gccgctcgat
    24421 atgcggcagg gtgcgcagct tgatgaggct ggcggtgacg gcggcgccgg tgtggccggc
    24481 gctgacgacg gcgtcggctt ttttgtcctt caccaggtcc acggcgcggg agatggagct
    24541 gtcctttttt ttgcgcacgg cgtcgaggcc gctgtcgctc atgtccacaa cctgcagggc
    24601 gggcacgatc tcaagcttcg ggctggcaat accctgggcg gccatctcgc gctcgatgac
    24661 ttccggcacg ccgacgagga gaattttttc gatccggggc agcgtttcga gggcgagctt
    24721 gacgccgccc atggggtttt gcggggcgtg gtcgccgccc atcacatcca acgcgatttt
    24781 catgcctccg atttggtctt taaatggata gggagtgtaa agcggaacga cggtggattg
    24841 caaacacgga aaagccgccg gaatggctca ccaaaagctg agcgcctcat gcaggtcgtc
    24901 catggcgcgc gtggcgctgg cgcgctcggg catgtccggg cagtcgaagg ggacaagggc
    24961 cacgcggttt tcacgcaggg cggtgatgcc gttgccgcag tccgggtcga ggagggactt
    25021 ccatgccttc tctgcgaagc gcgccgcgag gatgcagtcc tgcggggtgg catggcctcc
    25081 acgctggagg tggccgagga ccgaggggcg cacttcgagg tcggagaacg cgccctctgg
    25141 ccggtcgaag tattgctgaa aggcatcacg aagcacatag gcgccgttgc gcttttgcgg
    25201 cgggtcgaat ttcacgccct cggccagcag cacgatggcg tgtcctctgc cgcggatcat
    25261 ggcttcctcg atgcggtggg ccagcttttc gatctgcgcg gcatcgagcg ggccgccctc
    25321 gggtgtgatg accatctccg cgcctgaggc gagggcggcc atgcgcgcga ggtcgccgct
    25381 ctcgcgcccc atggtctcga gcaccatgac gcgccggtgg ctgcgggcgg tgtctatgaa
    25441 gtggtccacg gcccagacaa gtgtgtggac cgccgtgtcc acgcccaggg agagctcggt
    25501 gaactggagg tcgttgtcta tggtggcggg cacgccgatg atgcgcaggc cgctttcttc
    25561 ggcaaggcac ctggccccgg caagggaacc gtctccaccc acgacgacga gggcgcgaac
    25621 gccaagcgcg ccgaggaggg cgatgacttc cgcccgcacc tcccgctttt cgaattcctt
    25681 gcagcgcgcg gaaccaagga cagtgccgcc tttgcccatg atgccgctga ctgaggcgtg
    25741 gtccatgagc acgaccggct ggttttcgat cattcccttg cgccctggat ttctaacgat
    25801 ggcgtctttg agctgctgaa gctcgacagg agacgcgccc gcctggcgcg cggccttgac
    25861 gaggccgcgg tagccgtcct tgatgccaag cacgggcaca ttatgtcggt tgagtccgag
    25921 cctcaccaag gccctgagga aggcgttcat gccgggagcg tcgccgccgc tgcaaagtac
    25981 ggcgaccggg ttttcgggaa aaaagtctgt catgaaggga gaagcccgcc gaccatcata
    26041 tctggcgggc ctccagtcat agcgaacgct ccaacgctgg caagcggcgg gtttccctgc
    26101 cgtgaaatca cgtcgtctcc atgccgcgcg ccatgactac cttgccggtg cgaacggttt
    26161 cgacgatctc aaatttattc aggagccgga tggccgcgtc gagtttgtcc gtgttgcctg
    26221 tgatcatggc aatgaggctg tcatcctgca aatccacggt cttgcctcca tagtgctcga
    26281 cgatttgcag gatctcggtg cgctggtcgt ggcctgtgga gattttcacc aggacgagtt
    26341 ccttggcaac ggcgtcgcgg tcggtgtgct cgatgcagtg gatgacatca atgagcttgt
    26401 tcacctgcat gatgatctgg tgcagtccct ccgggtggcc gctgatgccg atggtcatgc
    26461 ggctgtagcg cgggtcgcgg gtctgtgaga caacgaggga ctcgatgttg aaaccccggc
    26521 ggctgaagac attgcatatg cggccaagga cgccgggctg gttgttcaca tagacgctga
    26581 gcgtgtgaat gttgatgatg tcggcctgcg gggcgggctt tttgtcggtg gtggcgggga
    26641 tgcggtcgct catgattttt gcggggctgg tgtttgaaaa taaaacggct gccacttaga
    26701 acaactgcgg ttcggcagat gcgagcgcgt ggagccgcct gcgggcgagc tggacgtagg
    26761 tttcgtctct ctcaatgccg atccatttgc gctttaagcg caaggcggcg gcggcggtgg
    26821 tgccggaacc gaaaaaagga tcaagcacta aatcaccctc atcagtggag caaaggacta
    26881 gcttttcgat gagatcgagc ggcttttggg aggggtggcc gcacttctcc ttggaggtgc
    26941 ccttgaggct gggcacacgc aagatgttcg tcgcgaattt gcccttttcc aggtgggcct
    27001 tgcgcgcgtc catgttcttg taacgagtgt ctttgaggta ttgggcgatg gttttttcgt
    27061 cgtaaggaat gcgcacagcg tccttgttga acttcacctc atcgcctttg cgcaggacga
    27121 ggatcatctc atattgcggt gtgaagctgt cccgccgctc attgtagccc acggcgcgat
    27181 cccaaataat gagatcgtga aactgatgcg accgggagag ctgcgacatg aggtggagaa
    27241 aggtgtgctc gcgctttcct agctggccga atatgaaaca gagtccttca tcacaaagga
    27301 cgcgcatggc ctcgcccgcc cactcctcgc accaggcaag atagtcagcg gcggttttcc
    27361 attgggtgtc ccaggcttca tcttcgagaa cattgaaata aggcggatcg gcgataattg
    27421 cttgagcggc gcattccggt agcttgcgaa gttccgtgag ggcgtcccct tgaataatga
    27481 gattggcttt catcgttgca tcgccacact ttggcgagtg cgcctgcggt catcacgtcg
    27541 aaccggtggg tttttccatc ttgtgtttcg gcgcctcggt gagcatgtct tcaagggcgg
    27601 ctccagcagg gatcatggga aagacgtttt catgtttgat gcactccgcc tcaatgatgc
    27661 agggtccgtc attgtaatcg agcgcctgct ggaggatgcg gcccacgtcg gcgggacggc
    27721 ggatgtgcca gcctttgatg ccataggcct cgccaagctt gacgaagtcc gggttgccga
    27781 gcaggtccac gccgctctcg cggttgtcga agaagagctc ctgccactgg cggaccatgc
    27841 cgaggtagct attattgagg acgaggattt tgacgggcag cttgtggatg gccgcagtgg
    27901 ctagttcgaa cagcgtcatc tggaagccac cgtcaccgca cacggcaacg acggttttat
    27961 tcgggcatgc gaactgcgcg ccgattgccg cagggaagcc gaagcccatg gtgccggcgc
    28021 cgccggagct gatccagtgg tagctctcgt cgtttttgta aaattgcgcg gcccacattt
    28081 gatgctggcc gacatcggtg gtgacaatgg ccttgccctg ggtgagatgg taaagctcgt
    28141 caagcacttg ctgcatgcgc aagccgccct gttttttata gccgagggga tatttctttt
    28201 tgtagccgtc gagatgcgcc agccagtctc cggtgggcaa tttttcgacg aggggcaaca
    28261 ggtcgctgag tgcggccttg gcgtcgccag ttatgggaac atccgtgcgt atcattttgc
    28321 cgtgctcagc ggcgtcaatg tcaatgtgga tgattttggc gttgcggccg aacatgtgcg
    28381 gcttgccgat gatgcggtcg tcaaagcggg agccgacatt gaagatgagg tcggcttcgc
    28441 agatggcctt gttcgcgtag gcggttccgt gcatgccgag catgccgagg gcaagcggat
    28501 gcgtttcggg gaaaacgccc ttgcccagca gggtggtggt gaccgggcat tgcagtgtct
    28561 ccgccagcat gagcaattcc ttgtccgcgc gggcgatcat ggcgccctgc cccgccagga
    28621 tgaccgggcg ttgcgactgt gaaagcagcc tggccgcctc ttttatggca atttgatcaa
    28681 ttttctgcgc atcttccgga tgatagccgg gcaggtccat cggatcatca aaggtgcctg
    28741 tgaacgggct ctggctgatg tccttgggga tgtcaataag cacaggaccg ggccggccag
    28801 tggtggcgat gtggaccgct tccttggcga tgcgcggcag ggcattggtt tccttgacca
    28861 agtagctgtg tttgcagacc ggggcggtga tgttgaaaat gtccgcctcc tggaaggcgt
    28921 ccttgcccag catccaggtg acctgctgac cggtgaggat gatcattggc acactgtcca
    28981 tctgcgcggt catgatgccg gtgacggtgt tgccagcgcc aggaccagaa gtgacgagga
    29041 cgaccgccgg tttgcccgtg gcacgcgcgt aaccgtcagc catgtggaca gccccctgtt
    29101 cgtgacgaac gaggatgaac ttcatgttcg ttttcacggt ctccagggcg tcaaaaatcg
    29161 gcaaagcggc accgccagag tagccaaaaa catattcaat gccgagatcg gcaagtgttt
    29221 tgatcagcgc ctgcgcgccg tccatttggg gtgtgctcat aatatttcag taaaagtgcg
    29281 aactttcgaa cgaaagttcc attcattcag atgttcgcaa tctaaaaatg cgccttttcg
    29341 atcaaaccaa accaaacatg aggtagtctt cacttaaaaa tctaggattt agagtcaaaa
    29401 tcgagaaata cacgactcac ctcaatggat ttttgcagtt ttcgaaaaga ttcgtttttc
    29461 gacaagggcg gaaagaaaac acccggccaa attggccggg tgtttgggaa tccgcattga
    29521 ttgcgaacag atcaggcgaa gatgtccacg actggcacac ccttgtcgcc cacagtgaag
    29581 gggcggttgg atggggacat gacaacttcg ttgacaggca ggcccatggc gtgaccgatg
    29641 gtggccacaa agtcctgcgg agtggtctgt ttgtcggcgg ggcggcgtcc gtccttgtcg
    29701 gtggcgccat aggtgaagcc gcgtttggtg ccgcctccgg cgaaaacggt ggaatatgcc
    29761 agcgggtaat ggtcgcgtcc gtcgttttca ttgattcccg gagtgcgtcc gaactcggaa
    29821 ccgagcacaa ccagcgtaga atccagcagc cccttggctt ccaggtcgag cagcaatgcg
    29881 gagaacccgg tgtcaagcgt ggtgccgttg ttggtcatgg ctgtgtcaat cgtgttgtga
    29941 tgatcccagc cacccatgcg cacctcgacg aacctgacac cgctctctac cagcctgcgg
    30001 gcaagtaggc agccctgccc gaaagaggaa cgaccatatt tgtcgcgggt gtcggcggac
    30061 tcctggctca gatcgaaggc tttgagatcc tcgctcttca tgatcttcat ggtttcgtcg
    30121 tagaattcat tgtaggactt cacatcctca gtgtcaaagc gtttgcggaa ggcgctgtcg
    30181 aactcgttca tgagttccac acgcttgccg aacatggagt cactggtggt gggacggatg
    30241 ttttgcagac ccgattcagg actggagatc ggaattggag ccatggaagg cggaaaaaag
    30301 cccgcgccag gataagcact gccgctgttg accaccacgc tatcaggcag agtcacgccc
    30361 ttgatgcgac caaggaagtg gctggcccag actccgacca gcggatgtac gatggtacca
    30421 cggggttcgt aaccggtctt cataatgtaa gtgccggagt catggacgcc agtcttggaa
    30481 gtcattgagc ggatgatgct gagcttgttg gcgttttccg ccaacttggg caggtagccg
    30541 cccaagtagt caatgccctc cgctttggcc ttgaccggat cgctgaaacc cttggtcatc
    30601 cctgttttgg gatcaaaggt gtcaatgtgg ctcatgccgc cgctcatcca aagaaagatg
    30661 acgttttttg ccttgccgaa accgggtgtg ccgggcccgg tgatcatttt ttcagccgcc
    30721 tgcatcttgg cggagaggcc tgggaagagt gtgacgccga gagtggcgct ggctgccccc
    30781 atcatgaatt gacggcggct gtgatcgtcg agtttgagca gttctgattt catgagtatc
    30841 gttattttgg agttgtgtgt gagtgatgag tattactgaa caaagatgaa ttcacgggta
    30901 ttgatgagcg cccagatcat attgctgtag gcctcttcgc cagaggcgct aagttcgcgc
    30961 ttggcgatgt ctttttcctg caatgtcggg cggcgattca tgatggtcat gaacatgcgc
    31021 tccacttttt cagaggggtc tttgactttg tccatagttc ggaaaacgag ggaatcacga
    31081 tttgtcaaca tttgctgcgc tttgccgttc atcatcatga gcacttgtgg aacattgccg
    31141 atcgatgtgc tgccatcaat caaatcgcgg ggagactggc cgaagtcggc aagaaagtgc
    31201 ccacccggag cgggttgaac gatctccgaa gcgcgaagaa gccgcatacc ttcataggac
    31261 aggatttttc cggggccatc catctgggca gtagtagccg cttccgcacc cttcggtgct
    31321 gcatccatca tcatgctgtc gtcaccagcc atttcaaggc cgccgcccat cagttcacgc
    31381 tctttttgtg ccatgcggcg ataggcgtca tatttcatga gcactgtttg cgcatccacc
    31441 gtttcgagat tgaggtcaat ggacttggaa tagagatcct ggagcggggc cttgtatgcg
    31501 tccggctgac ccagaacaag cgtcatatat gagtcccaag cttgctcggc acccatccgg
    31561 cgcagcatgg gcccctggaa gtaatagacc tcgcccatga cgatattttc ggtcgtcgcc
    31621 tccgactggt aggcacgggt attgtaaatg atgcgcatga attccttaag gttaaacttc
    31681 acacgcttca tctcttctgc gagatgcttg agcaattcgg gatttgctga ctggtcaggg
    31741 tcatcaatgt tagtgacagg ttcattgatg cctgcgccaa aagcacgctt ccacatgcgg
    31801 ttggcgatgg tcattgcaaa gcgcgggttc tgcgggtgcg tcatccaatt agcaaaagaa
    31861 gtccgaagct tttcctcctt ggctttcgac tgcttgtaag cgggattgct tctgtccccg
    31921 gaagaccagg tgacaaattt gggactcaca ggatccccgg gcttgccgtc cttgtatttg
    31981 taatcgtgcg ggagcatcat catgttttta tccaaatcac ggacgacata tgtgttcgcc
    32041 cccatgtagg tttggatgcc gttgcgaatg cggcgcatgt ccagaccggc tttttcaacc
    32101 agtttctcga cttccatcat gactgtttcg ttacggtagg gggcgcgtgt ggatgtggcc
    32161 ccaaagaagg ccgccatttc gtaaaactgc atctgcgtcc agtccgcgaa aggatgatcg
    32221 tggcattgag cgcaggcgac atcagtgcca aggaaaacgg tgagggtatt ggcaagattg
    32281 tccaggggca tgccggcatc gcgaagcaaa tagcccgccg caccgttatg ccacatcttt
    32341 ccactggcag tcatcatttc atgcaccatc tgatcgtagg ggcgattttt tgcaacctgc
    32401 tccttaaccc agttgatgta tggcgtgcca cgcagattcg cctgatcgag attgtctttg
    32461 aggcgcagca tctcggcgaa gtaattaaac atgtgcgctt tgaagccttc ggaatccagc
    32521 agcatgtcaa tcaaatcggc gcgcttcttg gggtttccat ccttgatgaa agccatggcc
    32581 tcatcatagt tcgggatgcg acccacgatg tcgagataga cacggcggac gaactgctcg
    32641 tcgttggcca tcgggttgaa ttgagtaatg ggcggtttgc cgtttttgac acgatccgga
    32701 ttggcgcgga caagaccctt ggccaccagt tcgttaatct tctgcgcggc ttgcgccact
    32761 gtcgcattgg caatcatttg cggttgcgcg gtatcggcag ccgctggggc ggaagatttg
    32821 gcgaaggcct gatcctcggc tgaaagcctg gataattcga accggtgctc ggctccatct
    32881 tgggtttgga gaacgatgtg ctcgccttca atccgcacca gagaggcgga gacttgacga
    32941 ccttggacat cagtccaggt gcgcacttca gccaatgcag aggaagtgcc aaatcccgca
    33001 aatagcagga cggcaatcat agaacgtgtg ggcttcatag ggagatgaga tgtattcgga
    33061 cgacgggggt aaccctcacc ttgaaacggt gtgaaaataa aatcgttagc gatttctaac
    33121 cgaaatttat tcgccaccaa tagctaaaac acaattttaa aatgcgttcc ttcgaacatt
    33181 cttggattcc ccttattccg gcagcgcttc gcaagtgaca acaccgcttt tgtccatcac
    33241 gcccagaact accgcgccct tgccgatttt tagagccttg cggacatgtt ttgcgtcaag
    33301 tatggccacc cagccaatgc ccatgttgaa ggtgttccac gcatccttgg gatcggcatg
    33361 acgcaggatt ttctgcgcca tgtcattttt ccaatatggc acctgcaagt gacacccaaa
    33421 gccgcgcctg gtgaagattc gaccaaggtt ttcgatcaag ccaccgcctg tgatgtgcgc
    33481 catggcggcg ggtttgatcc cggctttttt gagagcgcgg cattgcttgt ggtataatag
    33541 cgtcggcgct agcaacgagg cggcatcttt tgatccgaac tggatctttt ccttttcgag
    33601 gacgcgacgc acaagggaga agccattggc atgaaaaccg gcgctgggcc agccgacgag
    33661 aacatcgcct ttcttcacct cagcgggatt gagcacatgc tttttctccg ccgcgccaat
    33721 ggcgaagccg gaaagctcca ccattccctc catgaccgct cctggcattt cggccgtctc
    33781 accacccgcg aggatgcagt cgcagtcttg gaggtattcg gatattccgg cgatgatgcg
    33841 gctgagttgg cgcttaacga tcttgttcac gccgatgtag tcgaggaaca aaacaggatc
    33901 cgcgccggtg gtcagtacgt cgttgacatt catggccaca agatccttgc ccgcgttttc
    33961 cagaaggtca tgcttaagca gcaactccag tttggtgccc acaccgtcgc atccggtgaa
    34021 aatgactggt tgtttgtatt tgctcagatc atagcccgcc gcaaagagtc caaaagcatt
    34081 ggccagcttg cgctttttct gggtggcctt caccagcggc ccaatatcat ccacaaatcc
    34141 tgctgcttcg tgaatgtcaa cgccggcctg tttgtatgtg tgtttggaca tgattcattg
    34201 gccctcttgt cgggcgcaga attgagattg aagcagccgg gacaggatgc aagcgcggtt
    34261 tgacgctccc gaaggtcaat cctccttcat ttcacggtaa tggcggacga ggtagccgcg
    34321 ctctttgaga aggtgcagca gaccagtctc ggcggtgaaa tgaccggtgc ctaccaacac
    34381 catggctctt tcgccctttt tcagcaggtc ctcaagacgc gccatccacg cgcggttgcg
    34441 cgcggcgaga aagatttcca tcaattcggg gtgtttggca gcctctcgaa aaagcatgga
    34501 ctgaaggtcg tcaaggcgtc cgtgcttcca ggcttcgatc atcttgtcat actcctcagg
    34561 cagggtgccg acctcggcga gggtctggtc gagcatgacg cgctgcaact cctcggagag
    34621 gccggcgaac agctgaattt ggaactccag cgtttccaat ccctcaccgg gtttgccgtc
    34681 tcgccgcgcg cgtttctcaa aatgctggtc cactcccatg tcgggggagg cgcccagaac
    34741 cgcgtattcg ttgttggtga tcatgagaga ggccagccat gggcgcatcc gggccatgga
    34801 aaatggcggg acgccgcgtg acgcgcacca tttttttacc ccgctccaag attcactccc
    34861 cacctcggct tcgagggtgg cgcctggcgg cagggtgccg agctgcgtca tgcgcgcggc
    34921 agcgccagcc ttggccgcgt ccggcggaag ctcaaagacg agcttgtctg atctggagta
    34981 ggcagcctcg taggcgggag cgaggggata atcctccttc cgcaaaatgt gaatggtgcc
    35041 gcaaaggtaa agcctgcccc cagtgtcctg ggaatccacg acccatacgc tgccgatggg
    35101 acccgcctcc acggggtgcc ccccctgaac agactcggcc tgtccggcat cgcgtccgca
    35161 cgcgcacaca gcggccagga cagtcaaaca aagaaggcgg cggaggaaat gcacgcctca
    35221 ttgaaacacc tgacgcggca aaggggcaac aaggaaaaag ttcgcgccgc ggagcgggat
    35281 tcacacgccc gtctcgatca ttttcgcgaa ttcggcaaag aaatacttgg cgtcattggg
    35341 accgggcgcg gcctcgggat gatactgaac gctgagaaca ggcgcttcgc ggtgcctcat
    35401 gccctcaacg gttccgtcgt tgaggttgat gtgcgtgact tcgacattcg agggcaggct
    35461 ggaggggtca atcgcgaagc cgtggttctg gctcgtgatg gcgaccttgc cggtgcgcaa
    35521 gtctttgact ggctggttgc cgccacgatg gccaaacttc agtttaaagg tgcgccctcc
    35581 gtaggcatgg cccaggattt gatggccgag gcagatggca aaaaggggtt ttttgccaat
    35641 gagcgcgctc acctccttgt ggatgtaatc cagcgcggca gggtcgccgg ggccattgga
    35701 gagaaagacc ccgtcggggt tccgcgccag cacctcctcc gcggaggtgg tggacggcac
    35761 gacggtcaca cggaaaccgc tttgccgcag gccgcgcaga atgttgcgct tgatcccaaa
    35821 gtcgaaggcc acgacatgat gcctggcttc cggtagcgga tgaaaggctc cctcggggcc
    35881 ggcctcgcgg ttttgactcg gactgggaat gtcccaaacc cggctttcca tatcatcagg
    35941 atcccatgtg taggcggctc cagtggagac ttccttgacg aagtcgctgc cgctcatcgg
    36001 cgggctttca gccgcgacgc gcaccgcctc ctcgacgctg ccaatctcac tgctgatgac
    36061 ggcgcgcatg gcccctcgtg tccgcaaatg gcgggtgagg gcgcgcgtgt ccacgccctg
    36121 aatgcctggg atgtcccact gcctcaggta atcatccaga gtccctgtgc tgcgccagtt
    36181 gctgggcacc ccgcacagtt cctcgatgac gaagccgcgc acatgcggtt gcgagctttc
    36241 ggggtccagg ttgttgacac cgtagttgcc gatcatcggg taggtcatcg ccacgatctg
    36301 gccgcggtag gaggggtcgg tcagcacctc ctggtagccg ctcatggacg tgttgaagca
    36361 cgcctcgcct gttcggctgc cgggagcgcc aaaggattcg ccttcaaaat accgtccgtc
    36421 ttcaagtgcc aggattgctt tcatcaaaaa tgggaaactt gcgcacctag cgcgcgcggc
    36481 ggaggaatgc aagcgcgcgg catccccggc tttcgttcgc ccttcatcgc gcctttccct
    36541 ccgccatgtc gtggctgaaa aaaacggcgc attcgcccgg tgccgcacgc gcatgaggat
    36601 gcccgctgga cgaatcctgc ccgaggcact aaatctccct catggaccgc aagctcatca
    36661 atcgcgaaat cctgcaatcc ttctggaaaa tccacatcct gcatcatgcc agcgaaggcg
    36721 gggtgatcgg ccagtggatg ctcaaagaac tggcccatca cggctacgcg gtgagtcctg
    36781 gcacgcttta cccgctgctc aaacgcatgg agggccacgg ctggctcaaa tccgaaacaa
    36841 atcccgcgcg ccgcgggcca aaagcgccgc gcacctacct aatcaccgcc gagggcaaaa
    36901 aagtgctcaa agtggtgcgc cagcaactcc gcgaactcgg agtcgaggtc ggcggggatt
    36961 gacccgccgc gatcaaggaa tcctgccgaa cgcatcggag acgttctcca ccgtcagtaa
    37021 ttccggcagc acatgcggat ctgtttgtcc gggaatgtac agcacattca caggcacggc
    37081 ggccttgccc agcgcggcca gcgcctcggt gatgcgggga tcctcgttgg tccagtccgc
    37141 cttcagcaac gcgacttttt tttccgcgat gaaacgctgc agccctgcgt ccttatagac
    37201 gcgcttgttc acctggcagg tcaggcacca gcgcgcggta tagtccacat acacggccct
    37261 gccttccgcg cgcagcgtat ccaccagttc cggggaccac gcctcccagg tcagaccgtt
    37321 ctccgtggag gcgccgcttg tcacaggccc tttttccacc tgtggccagc ccagaaacag
    37381 cccgccggcc accgccgcca gcgccagcac caccgcggtc ctgcgtgtgc gcgcgggctt
    37441 gtgcggcggg ctccagcgcc cgtaaatcca gcagcccagc gccaccacca cgagaccgag
    37501 cagggtgaaa agcagcggct ggccttcgat catgccggcg agcacccaaa cgaagtaagc
    37561 cgcagtgccg aacaaaagga acgacatgcc ctgcttgaaa ctctccatcc acgcgccggg
    37621 acgcggcagc gcggaaacca ggcccggcat cagggaaaga agcagaaacg gactcgccag
    37681 ccccaggccg atgaaggtga acatcaacag cgcttcgagc accggcaatg tgacggcaaa
    37741 tcccaaagcc ggaccgagaa aaggggctga gcacggagtg gccacgacgg tcgccagcaa
    37801 gcctgaaaaa aacgagccgc ccagcccctg acgcgcctgc aaatccgcgc ccacacccac
    37861 cgctgacgcg ccaatctcga aaactcccgc catgttcagg gcgaagacca ggaaaaaggc
    37921 gcacatcgcg agattgaacc cggctgactg cagctgaaag ccccacgatt tgcccagcac
    37981 caccaccagc gatcccagcg cccagaagca aagcagaatg ccgaaagtgt aagccaggcc
    38041 gtgcagcaaa acacgcctcc ggtcctcccc ggcttgctgc accaccccca tgattttgat
    38101 tcccagcacc ggaaacacac acggcatgac attcaggatc aatccaccga taaaggcaaa
    38161 aacgagcaac actccaaacg acctggcggg cgcgaccgtg gtcttgttct ctgcagcggg
    38221 tgccatgctc ctggcaaata actcccctgc agcctcattg agcaccacct tgtccgccag
    38281 cgtgattgac agccgcgcct ccgccttggc tggcatgcag ttctgcggat cgcaagccag
    38341 cgcgtcaacc ttcacgacga tctcaccggt gtcccctgcc ctgcccgcag gaatgatcct
    38401 tgcaggcaga tgcaccgtgc cgtcgtatcc ctcgctcgtt ttgccatccg tggagggcac
    38461 ctcatgcgtc gccggccagg gcatgtcctc caccttccat ccatcgggca aagtccagat
    38521 cagcctggtc ggcttgccga tcacctccgg cggcagctcc ttgccatagg tatgccagcc
    38581 ctcctcatgc accagcctca ccgccacctt gaaaggcgcc ccgggcgcgg cctgggtgac
    38641 ctcggcaaca agcccggctg tcaccttggc tttcgcgctg gccgcgccaa aatcaatctg
    38701 cgcatgaatg gcggaggcgc agagcagcag gccggcgagg gcggggaaaa agggcagttt
    38761 ggtcatggtc ggcatacgag acaccaacgc gcgctgtatt ccaagcctcg cctgatgcga
    38821 tggcatggaa acgacatgcc ttccgcctca cacccccgcc agcttcgggt tcgcgtggcc
    38881 ttcgttttgg tcaatgcgct cggggcggcc tttgtggtga tagacgagct gcgtgtggtc
    38941 cacgccgagc aggtgcagga tggtggagtg gatgtcatgc acatgcatgc ggtcctcgat
    39001 ggcgtagagg ccgagctcat ccgtggcgcc gatggtctgg ccgcctttga caccgccgcc
    39061 ggccatccac atggtgaagc cgttcgggtt gtggtcgcgc ccgccgccct gctcgctcat
    39121 cggcgtgcgg ccaaattcac cgccccagat gacgagcgtt tcatccagca ggccgcgctg
    39181 cttgaggtcg ctcagcagtc cggcgatggg cttgtccacc gcacggcaga gtcggctgtg
    39241 attttcctcc aggcgggaat gactgtccca cttgctccca gcgccgctgt agagctgcac
    39301 aaagcgcacc ccgcgctcca caaggcggcg cgcgagcagg cagttgcggc catagacggc
    39361 ggtctcgggt tggtccatgc cgtagagcgc tttcgtggca gcgctttcct tgtcgaggtc
    39421 caccgccaca ggcgcggcgg cctgcatggt gtaggccagc tcataggagc gaatgcgagc
    39481 ctccagctcg gtgttgtcgg cgcgcgtggc attgtagtcg gtgttgagcg cggcgagcag
    39541 gtccagcttg tcgcgctggc gctgctgggt gtggcccttg gggttgtcga ggtatttgat
    39601 gggcactccc tcgccgctga actccacgcc ctgatgcacc gcgggcatga aaccgctgcc
    39661 ccaattgcgc acgccgttga ccacggtgcc ctcgctgtcc ttgatgacga caaagccggg
    39721 caggtcttgg ttctcgctgc cgagaccgta gttcacccac gcgccgagtg atggacgccc
    39781 gccgaagatg ctgcccgtgt tcatctggca cacaccaccc gcgtggttga tgccatccga
    39841 cacgcacgag tggatcacgg caaggtcatc cgcatgacgc gccacgttct cgaaccaatc
    39901 cgaaatccac agcccgctct gcccgtgccg cttccacgcg cgcttggact cgagcaaggg
    39961 cgcgtcgctc tcccccatgg cgagcacggg caccttgaaa ctcggcggca gtttttgacc
    40021 gtggatctgg ttcagcagcg gcttgcggtc aaaggtgtcg agatgactcg gcccgccttc
    40081 catgaagcaa aagatcacgc tcttggcctt gccaaagcgg gagggaattt tcgcgctcgt
    40141 ggccgctccg gcactcggcg cgccgagcac tggctcgttc atgagggcag caagcgccac
    40201 cgcgccaaag ccgccgccgg cgcggctgag gaactcacgg cg
//
LOCUS       DAOMYJ010000020        39196 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Prosthecobacter sp. isolate AMR_MDS_5629
            NODE_389_length_39196_cov_19.262972, whole genome shotgun sequence.
ACCESSION   DAOMYJ010000020 DAOMYJ010000000
VERSION     DAOMYJ010000020.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867850
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prosthecobacter sp. (wastewater metagenome)
  ORGANISM  Prosthecobacter sp.
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Verrucomicrobiaceae; Prosthecobacter.
REFERENCE   1  (bases 1 to 39196)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 39196)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.30
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:34:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,742
            CDSs (total)                      :: 4,684
            Genes (coding)                    :: 4,676
            CDSs (with protein)               :: 4,676
            Genes (RNA)                       :: 58
            tRNAs                             :: 55
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..39196
                     /organism="Prosthecobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_389_length_39196_cov_19.262972"
                     /isolate="AMR_MDS_5629"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1965333"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..787
                     /locus_tag="PLP58_03645"
     CDS             <1..787
                     /locus_tag="PLP58_03645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ07649.1"
                     /translation="LAAAAAPGLAMTPSLAWLEWGLRTPWVTIPVAVAAFLIVWLSSH
                     AVNVLIALSPFGIVDSALKLFKLAIFTLVFAITAVSAWAGIAFCLLLILLAMLVAGWS
                     FRLSVFGALMGRDFLLNKHATAGELDDGVKGFLSRSTAGVPARTYGRMENDAGGSAGF
                     AYRPWLILPEKRILLPAEGRVLCKGVLHPGLLHRDAPDAAEKTRLRGCLILLPRYRGL
                     EESIAARHGCQEIVDSALLRGFKAVRHWFVEMLALGRPRERLA"
     gene            836..2125
                     /locus_tag="PLP58_03650"
     CDS             836..2125
                     /locus_tag="PLP58_03650"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HRJ07650.1"
                     /translation="MSSLFIWFRLLFNCRFYYPVFTILFLDLGLSIAEFAALNVVWAV
                     TIVLLEVPSGALADRFGRRPLIVAAGWLMVAEMAVLCLMPVGDHGTVLWLFVLNRILS
                     GAAEACASGADEALAYDSLPEQDRAALWPAVLARLSRAMALGFIVSSVTGAVMYDHAK
                     VSALLAWLGMDAMPRPLTLKLPLLMNLLTGVACLLVALRMREPAAHDTRPRLPWTAET
                     ARALRGMFGTGLWIWRRHDVFRLLVLAVVFDSVIRLFLTVASQYYRLIGIGEAWYGVI
                     GTTAALLGLASAGMMEKMTLRSTLRGNFAFLALLTLGGLACAAAAAPGWAGVALVMPV
                     MLAMRFLQFFLSHYLNAETGSDRRATVLSFRGLATNLAYGGMTLLFGWHTGWLKNARE
                     PPADDLIVFADSLKLWPAWFAATLLLSLLLITWHSRR"
     gene            2655..3863
                     /gene="wecB"
                     /locus_tag="PLP58_03655"
     CDS             2655..3863
                     /gene="wecB"
                     /locus_tag="PLP58_03655"
                     /EC_number="5.1.3.14"
                     /inference="COORDINATES: protein motif:HMM:TIGR00236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008761 - UDP-N-acetylglucosamine
                     2-epimerase activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase
                     (non-hydrolyzing)"
                     /protein_id="HRJ07651.1"
                     /translation="MEVVKEPGRPLLLFCLGTRPEMIKLAPVILLAKQRGMDTLLVNT
                     GQHADLLSPLFDLFGLHPDHDLTVMCANQTLNGLLARILERLDPILEKAAPSHVIVQG
                     DTATALAGALAAFNRKIPVAHVEAGLRSGNRLSPFPEEMNRRLVTEIADLHFAATTLN
                     RDTLLAGGVPAERIHTTGNSVVDALHHTLARSKPGIDLLALRRQVAGRRVVLLTTHRR
                     ENFGDTMRAYLRSLRRFVEAHPDLCVVFPMHPNPNVRAAAEAELGGCDQVIITSPLGY
                     ADFVHLLNDAWLIVSDSGGIQEEASALGKPILVLRENTERPEGVNAGVARLAGEQPDQ
                     LDHLLGGAVADKAWFEQAAKAEKVYGDGRAAARILDVLAGPAPVKAAPAHSPALLHGF
                     AAAAPLLQAS"
     gene            3860..4816
                     /locus_tag="PLP58_03660"
     CDS             3860..4816
                     /locus_tag="PLP58_03660"
                     /inference="COORDINATES: protein motif:HMM:NF013672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HRJ07652.1"
                     /translation="MNDRAASVSIDLDNQWAYMKTQGCAGWEEFPSYLEKVAPRILET
                     LRLCGLRATFFIVGKDAALEKNRAPLRAIADAGHEIANHSFLHEPWLHLYDDTELRRD
                     FNQAEESIAAATGARPQGFRGPGFSTSPAVRALLRERGYVYDASILPTFIGGLARAYF
                     FMTSKLPAEEKKRRSRLYGRFSDGFTPLRPYAIEPGLIEVPVTTLPLLRTPIHLSYLL
                     FLGQYAMPLAHAYWHSAVAACRAAGVGPSLLLHPTDFLDADDVPAMRFFPAMRLPSAR
                     KIALVRLTLESLRAHWSTGTLLRHAQSLAGENTACLQKVQAC"
     gene            4839..5633
                     /locus_tag="PLP58_03665"
     CDS             4839..5633
                     /locus_tag="PLP58_03665"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HRJ07653.1"
                     /translation="MSTAASIQEVAVVLPAFNEEKDLPNLLARIRETLTAQPFKYQVI
                     VVDDGSADRTAEIAREAAAAMPLRLIQHEKNSGLGMAIQTGLSAAMKCADMVVVMDSD
                     NTHDPIHVMEMVAEMEGGDTDVVIASRFQPGSVVIGVPAYRQLLSIGCFLLMKVLVPY
                     RGVRDYSTGFRGYRAGILQRLAAAHGEDKLVEESGFVCMLEVLLKLRALGARACEIPY
                     TLRYDLKAGASKLRIWRTLKRYLAVVARHRSSKTEPALMKVAEARA"
     gene            5660..6955
                     /locus_tag="PLP58_03670"
     CDS             5660..6955
                     /locus_tag="PLP58_03670"
                     /inference="COORDINATES: protein motif:HMM:NF005560.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HRJ07654.1"
                     /translation="MNSERTLILGGGISGLACALRLAGAGRKVTLVEGSDRLGGLGAF
                     FEHEGRTFEKFYHCMLPSDGPLLALLDSLGLTPEIYWKPSTFAYAGAGRIFPLNTPLD
                     LLRFAPLPFADRIRVGLTGLWGRLCSDKGLDDLSTVDWLTRLSGRRAFETFWKPMLEA
                     KFGDRHQDVPALWFWTRFNREKGESKGEVKGYIRGGYKRIIERAAERLRELGVEIRLG
                     ESIQSADLDENGSPFVITARGREDAARLLITLPWPGLAQVTGPRLRQAAQVPDWGIDF
                     QGVVNHLLFLKRPLTPHYWLATPESQFPFDGVVETSALTDEADRGTGRHVVYLTKYLH
                     RDDPRFAQPAEEIHRPWFAALQRLFQDLTESDVEAAHTFRAPYVEPVYTRGFLKRRPP
                     EVLVPGKVFLATTAQVYPVVTSWNGSVIQAGRTVDAMLL"
     gene            complement(6995..8260)
                     /locus_tag="PLP58_03675"
     CDS             complement(6995..8260)
                     /locus_tag="PLP58_03675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ07655.1"
                     /translation="MSASHHPHTHAVRRNFICHCLEGGLYMGGTAFLAPESVLPKMVE
                     MLGGRAWIIAMMPALLPAAFAIAGIFIAPLVEKLPRFKPWVLFFGLLQRLPYLVTGLI
                     LMFADRVDGALLAIVVLTPVVSGLLGGVTVVAWMEMVTRMVPEKARAAGWAVRYIIQA
                     VIGVLAGSVIHHVITHYPGREAYAWLHLAAFALLFVSWLSQLFMHEDEHTRHRHPAPP
                     AGPYLAYLRGLPAMLRAQPRLVRLVLARFTGMGYLMMVSFLTIHALHVTGRPEADEGR
                     FVTVQAAGTVLGSLLAAYVGYRSGGKVLLQASRVVCIALALWVSFTGQFGGFLLAYFA
                     LGFGLFLDRVGDLTLAAELCPLERRSTLQALLGFCNVFALLLATFISGQIYHFTGSFQ
                     AVALTTVGMSLLSIWILRHIPEPRVAQQG"
     gene            complement(8281..8703)
                     /locus_tag="PLP58_03680"
     CDS             complement(8281..8703)
                     /locus_tag="PLP58_03680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009961504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketosteroid isomerase-related protein"
                     /protein_id="HRJ07656.1"
                     /translation="MPNPQEAALQLVGEYYSTFNSGDRAAMLDLLSENVVHDINQGGS
                     ETGKAAFRAFLQRMDRCYREQVVELEVMANAAGTRAAAEFFILGAYLEGEAGLPPAMG
                     QNYRLRVGAFFDIAGGRITRVTNYYNLEDWLRQVRAPA"
     gene            8837..9607
                     /locus_tag="PLP58_03685"
     CDS             8837..9607
                     /locus_tag="PLP58_03685"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HRJ07657.1"
                     /translation="MTHPLFDLTNRAALVTGGSKGLGKAMARGFAEAGADVFISSRHE
                     DELKAAAAEIGEGLSVKVEWMVADMCDRPQVRALADEAVKRLGKVDILVNNAGSNQPQ
                     AIDEITDEAWDRIVELDLTSCMALTRYLVPGMKERKWGRVIHISSVLGVGSKEKRNVY
                     SACKAGLIGLAKASAIDLGAHNITVNCLCPGPFLTDLPGSLLSPAEKQAFADRTALKR
                     WGHPRELAGPALMLASEAGSYITGEALLVDGGAFARAL"
     gene            9650..10921
                     /locus_tag="PLP58_03690"
     CDS             9650..10921
                     /locus_tag="PLP58_03690"
                     /inference="COORDINATES: protein motif:HMM:NF025943.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide lyase 6 family protein"
                     /protein_id="HRJ07658.1"
                     /translation="MRLLLCLPLIFSIVHSAEVAVANVDELNAALRQARPGDTLVLAE
                     GEWRDTVIRCNAQGTESMPVTLRAAVPGKTVLTGQSALRLGGRHVVVEGLWFRNPDPA
                     AGDTVEFRLDSKNLARHCRVTNCAITMDAGQSARDDKESRWVGIYGGHNRLDHCLIEG
                     KVTKGASVVVWLGEGQEAAHRLDHNHFGPRERLGKNGGETLRVGDSKTSMSAASCIVE
                     DNLFERCNGEAECVSNKSCGNTYRRNFFLEVSGTLTLRHGNACLVEANTFLGNGANGT
                     GGVRIIGEDHVVRGNHFEQLAGDDARCAIVIMQGVPGSALNEYFQVKRARVESNTLLD
                     CEHPILIGLKDGRGSLPPLDCSFTSNRVLAPKSAVVEARCDISGVRWQGNVFHGIAPG
                     ISVSEGIEWQPPSIASAGEPERRGYGPAWRR"
     gene            10985..11650
                     /locus_tag="PLP58_03695"
     CDS             10985..11650
                     /locus_tag="PLP58_03695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ07659.1"
                     /translation="MKNARSLAAAFIFPLCAASVLRADSAGYKPELFESVRLVYEDSF
                     DGALNTEFWEVRQSSTWKVADGVLTGSQSSKEFQEKKIAEGDKAHAGFKPVIWLKQVP
                     ENFVCVMRMRYDAEAWQPRFPLLDIGHHIHTLVFSEKQTTLAIKKNAEIVAVESPLFS
                     LNEWHDVAIEVRKGRLLLVIDGKKRLFESPHIDMTGHAQIDFKGVDFGACRIDRIQLW
                     EGR"
     gene            complement(11664..12311)
                     /gene="nth"
                     /locus_tag="PLP58_03700"
     CDS             complement(11664..12311)
                     /gene="nth"
                     /locus_tag="PLP58_03700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018971080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HRJ07660.1"
                     /translation="MTKAQRARHVMTRLEELYPETPVPLDHKDAFTLLVAVLLSAQCT
                     DARVNQVTPALFALGDTPEKLARAEVAEIEKVVRSCGLGPQKSKAIQKLSEILVAEHG
                     GRVPACLESLERLPGVGHKTAQVVMAQAFGVPSFPVDTHIHRLAARWGLSSGRNVTQT
                     ERDLKSLFPQSAWNKLHLQIIFYGREHCTARGCDGTRCEMCRTLFPGRKPPPRKR"
     gene            complement(12324..12827)
                     /locus_tag="PLP58_03705"
     CDS             complement(12324..12827)
                     /locus_tag="PLP58_03705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009962421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3D domain-containing protein"
                     /protein_id="HRJ07661.1"
                     /translation="MMRVSTLPLLCLLAALALCSCASSNAGKSKIVTTKGTRITSVRT
                     TAYTHTESDHIQYGARTAVGTGLRHGTVRSAAADWSVYPVGTIFQIEGSPYLYQVDDY
                     GSALVGTNTIDIYQPTRAHMNAWGVRNVNIRVLKWGSFSKSLAIMKPRAHHPHVKRMV
                     DRIGRSS"
     gene            complement(12927..13394)
                     /locus_tag="PLP58_03710"
     CDS             complement(12927..13394)
                     /locus_tag="PLP58_03710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ07662.1"
                     /translation="MKAPALLRAALCLTAALIFTQCASPVQKRIQRNPEIYARLSETD
                     KLLVQQGRLREGMTREAVFLSWGRPDQVASGSQRGAQTERWTYLGAQPVYTDTWNMGW
                     GWGGRGWGRGWGYGGMWDPFWGGYGSMVTYVPYKAASVSFRGGRVTEFLRGPQ"
     gene            complement(13461..13946)
                     /locus_tag="PLP58_03715"
     CDS             complement(13461..13946)
                     /locus_tag="PLP58_03715"
                     /inference="COORDINATES: protein motif:HMM:NF025054.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HRJ07663.1"
                     /translation="MSDAAPISIRHYRPEDLEILRRITVDSFGSVALDQMLEDRLGVW
                     NGRDWKARKADHIDEDCTVNPEGCFVAERQGEVLGYITTRVDRVNSIGRIPNLAVVEA
                     ARGLGLGRRLIHHALDYFREQGLQAAKIETMASNVIGQSLYPSCGFEEIGRQVHFAMR
                     L"
     gene            complement(13953..14603)
                     /locus_tag="PLP58_03720"
     CDS             complement(13953..14603)
                     /locus_tag="PLP58_03720"
                     /inference="COORDINATES: protein motif:HMM:TIGR02594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02594 family protein"
                     /protein_id="HRJ07664.1"
                     /translation="MSQYIVQPGDNPSKIAKKFGLTLAQFQRLNPGLCTFGSNECRVI
                     FPGQVVQVSGTVTGLPAEGAQPGAAGGAGFAAPPPWMQIAIMEVGVQEWQPGDNPRIQ
                     QYLASVGLPNKPDETAWCAAFVNWCLEQAGFRGARSGRVSDWWGWGRPVAATYGAVCI
                     LQPLGSDQATSGHIGFLHAMDATNVWLLSGNSKNSVRISAYPKNKLIHNAPYRWPY"
     gene            14709..15494
                     /locus_tag="PLP58_03725"
     CDS             14709..15494
                     /locus_tag="PLP58_03725"
                     /inference="COORDINATES: protein motif:HMM:NF012993.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrilase-related carbon-nitrogen hydrolase"
                     /protein_id="HRJ07665.1"
                     /translation="MPDALTLHLVQLDSQWEDKAANFARVRDLIARAMPAPGGLIILP
                     EMFATGFSDRLAATLEPLDGPVTAFLRDIASRTRCAVVGGLVTPAPDGRGFNQALAIA
                     PEGSALARYSKTHPFALGGEDSVHAPGGPPVIFEWGGLKISPLICYDLRFPETARSAL
                     ALGAEALLYIASWPVRRQDHWITLLQARAIENQAYVIGVNRCGNDPRHSHGGRSLIAD
                     PHGVIIADAGAQERVITARVEPALVREWRAQFPAAREYLALQP"
     gene            15513..16037
                     /locus_tag="PLP58_03730"
     CDS             15513..16037
                     /locus_tag="PLP58_03730"
                     /inference="COORDINATES: protein motif:HMM:NF015639.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HRJ07666.1"
                     /translation="MPPDPPPPETADTAPGSLCTACGLCCDGTLFHIVRLQPADSARQ
                     LSALGMKLRRRKKDPYFTQPCAFLHDCRCAIYEHRPQRCRHFECRVLQRLQNDEITPV
                     AAHALIRQARQIACGIEVQLASGDDTAAHLPLLERCEAALAAIPSGTPASSNLSAALQ
                     ELQRLLRAEFHALG"
     gene            16161..17987
                     /locus_tag="PLP58_03735"
     CDS             16161..17987
                     /locus_tag="PLP58_03735"
                     /EC_number="4.1.1.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018970543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004611 - phosphoenolpyruvate
                     carboxykinase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0017076 - purine nucleotide binding
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (GTP)"
                     /protein_id="HRJ07667.1"
                     /translation="MSKTNPSKPLSKNIHLLRWVKKMAALCKPDRIHWVDGSKAEYDR
                     LCEQMVEGGTFIKLNQKQWPGCYYAKSDPSDVARVEERTFICSHSKDGAGPTNNWEDP
                     FEMKKKLRGLFNGCMRGRTMYVLPFSMGPLDSPMSQIGVQLTDSPYVVVNMRIMARIG
                     KKVFQIIDKGEKRVVPCMHTVGAPLKKGQKDVSWPCNKEKYIVHFPETREIWSYGSGY
                     GGNALLGKKCFALRIASAMARDEGWMAEHMLILGVENPKGEKTYVAAAFPSACGKTNF
                     AMLIPPAHFQKQGWKVWTVGDDIAWLKPGPDGRLYAINPEAGFFGVAPGTSDHTNPNA
                     MAALRKNTIFTNVALTPDGGVWWEGMTDEPPAKCTDWQGNQWTPEIAREKGVKAAHPN
                     SRFTAPAAQCPTIDPDWEKPEGVPISAVIFGGRRATTMPLVYQSFNWSCGVYIGATMG
                     SEMTAAAAGTVGKVRRDPMAMLPFCGYNMGDYFRHWISMQRRLTETPRVFNVNWFRKD
                     KDGKFLWPGFGENMRVLKWIVDRCTGRGIAKETPAGWMPHYEDMDWTGLDFPKEKFDE
                     IQRFDRDAWRQEILLHEELFLDLRSHLPKEMIYERELLICRM"
     gene            18177..23996
                     /locus_tag="PLP58_03740"
     CDS             18177..23996
                     /locus_tag="PLP58_03740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018970223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit A"
                     /protein_id="HRJ07668.1"
                     /translation="MPKKAPVKAKPAAPADVIQVRGARQNNLRGIDLDLPLGKLCVVT
                     GPSGSGKSSLAFDTIYAEGQRRYVETFSPYTRQFFERMDKPKVDAIHGIPPAIAIEQV
                     NNIRSTRSTVGTITEINDYLKMLFPRVAEASCPECGKPVRPESAESILHTLLHQHSGA
                     QALISFGVPVPKGTKPEDFFPFLQSQGYLRVWLYGEVLRTDEPASLKGKKLPAIVLVI
                     QDRVTLTLENKARISEALEAALRLGKGTTTIVFPENPQSAIRSPQSFSTDWRCADCDL
                     KLPAPTPGLFSFNSPIGACPACKGFGRTLGLDIHKAMPDESLSISEGLVRAFGGSTYS
                     ESQDDLERCARKRGVDLDTPFCDLPADQQKWIIEGEDSDPERAWKRGLWYGVKGFFKW
                     LEARAYKMHVRVFLSRFRAYTTCGDCDGGRLKKEAYNFRANGRTIADLWNLPVTDLLP
                     FIQSWPITGSDSAALMLRDEICARLNYLDRAGLGYIHLDRQTRTLSGGELQRVNLTTC
                     LGASLVNTLFVLDEPSIGLHPRDIGRLIGVMEGLRDKGNTLLVVEHEEAVMRAADHVI
                     EIGPGRGDKGGTLVYDGPLAGLAEAKDSLTAAYLTGSKTIPLPEKRRQISNFEFRASP
                     PSKPAKSKSKKTAPPAFNPGFLSIVGARQNNLKNLDVDIPLGVFCCITGVSGSGKSTL
                     IHEVLYRNLQRLRGEVCEDEPGRVRSILGHEQLSEVVMVDQSPLARTPRSTPAVYVGA
                     FDTLRALFAESEEGRALGITPGFFSFNSGEGRCERCAGTGYEKIEMQFLSDLFVTCPE
                     CQGTRYQPHALKIKLDGKSIHDALEMTLDEAVAWCRSGVSPDPCEPGILPGSKTQSKR
                     FAQLARQLSILCEAGLGYLKLGQPLNTLSGGEAQRLKLVGHLLNSPSAGKMPASHESG
                     KMPDPPRKPLLLLDEPTTGLHFDDIALLVKLLQRLVNEGSSLLVIEHNLDVIQCADWI
                     IDLGPEAGAEGGQFVFTGTPEDLLLCRESWTGKFLAEKFENRNSKSESLTLAEAPAKY
                     AASKLRASNLEYSNSISIRGAREHNLKNISLEIPRDEFVVVSGLSGSGKSSLAFDLVF
                     AEGQRRFLDSMSVYARTFVEQMEKPEVDLISGVPPTVAIEQRISRGGGKSTVATVTEV
                     WHFLRLLFSKLGTQYCPRCEVPVQKQSLGAITQTVMARLKKGPCKLLAPLIKARKGFH
                     TEVAEAARKQGITTLLVDGELRDTMNFKKLARFKEHNIDAVVAELDGSLDELALRPHL
                     EKALKIGKGTLKLRLPDKSFQVLSTEMSCPSCELSFEELDPRLFSFNSPHGWCPACRG
                     FGFEVAAHGATPHRGDVSQLEAELEEERRLSRAADEDEENGERVTCKACNGSRLNATG
                     RAVRLQDRRIQEINAMSAADAAGIVARFRFDGREAVIARDILKEIEQRLHFMKEVGLG
                     YLQLDRSADTLSGGEAQRIRLAAQLGSNLRGVLYVLDEPTIGLHPRDNERLLDTLSAL
                     RDKGNSLLVVEHDDDTMRRADTIIDLGPGAGRFGGEVIAQGPLKALIKDKRSVTGQAL
                     REPMKHPLRGSRRPIWGTGVPPVSSKKKTGKMPVPHAEWLVIEGAHANNLKSIEVRIP
                     AGRLTVITGVSGSGKSTLLHQVLHPAVKAALSKKKAATDSEPLWKSVQGAGQFAAVYE
                     VDQSPIGKTSRSCPATYVGIMDDIRKLFAQLPTARARGYDAGRFSFNTDGGRCETCGG
                     NGVIKVEMNFLPTTRVHCEVCHGLRYNSATLEIEYNGKHIGDVLRMSITEAAEFFSSQ
                     PRLARPLQLLADTGLGYLQLGQPSPTLSGGEAQRIKLVSELSGGVSRSATEKLRGLTN
                     TKKNLYLIEEPTIGLHISDVARLLDILHRLVDDGHTVVVIEHHPDLFAEADYLIDIGP
                     EAGAEGGQVVASGTPEQVAKHKTSRTAPFLSRLLTQGRA"
     gene            24132..24938
                     /locus_tag="PLP58_03745"
     CDS             24132..24938
                     /locus_tag="PLP58_03745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ07669.1"
                     /translation="MNFLDSVRDLQGTALFYPCSGYDWLEPILTFLPVVKSFWFADPR
                     LHRSGSIPIEPFLEAASESADGPLAQGRRCTTRRSRDPGRNASRPSFVRRGTSLCLAD
                     HTFKRLSWEVREQSPVTESSKPDPDGNETPWVYAPLTVSERYLDASSGAEFAIHAHRC
                     DGQRSLDLLPEPLGIFYLRGDNGSGNCEGAEGSSGSGWLTRSWFEPVLRKLVPGGLVV
                     TDGSCSGDGKDYPGLCEFFLQPKRWRGCMEKGQAFHHTLRASFRVCGLCG"
     gene            complement(25496..27520)
                     /locus_tag="PLP58_03750"
     CDS             complement(25496..27520)
                     /locus_tag="PLP58_03750"
                     /inference="COORDINATES: protein motif:HMM:NF027900.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5060 domain-containing protein"
                     /protein_id="HRJ07670.1"
                     /translation="MHPRLFLIILCAALPPVCAGAADAPKFPAHWGEPPLRQTRDMRE
                     LPGGYGTGSSTLAKWIQEHLDRDQAARGGSDGDGSITLRGELRQWHKVTLELDGPFAR
                     ETGTAPNPFTDLAFEVTFTHESGAPSHRVPGHFAADGNAAETSADSGTVWRAHLSPDK
                     PGKWTYTTSFTQGARAALDGGGEALAPWHGRTGAFVVAPTDKTGRDFRAEGRLQYTGG
                     HHLQFAGSRRYFLKAGPDAPETLLAYADFDGTEARQKNVPLKTWKPHLQDWRQGDPSW
                     QGGKGRGLIGALNYLAAKGCNAFSFLTYNAGGDGDNVWPFTARDARLHYDCSKLDQWC
                     IVLDHAAALGLYAHFKLQETENDDERRGSKREKRPVSTALDGGGTGLERKLYLRELIS
                     RFAHLPALNWNLGEENTQTPDEQRAMAEYIARTDPYQHHRVIHTYPGDQEKVYRPLLG
                     GQSSLTGASLQNAWDDTHRRTLQWIRESAAAGRPWVVASDEQGGADTGVPPDPGYQGY
                     DGKSGGKVIQTPDDIRKHTLWGNLMAGGAGVEYYFGYKLPENDLKCEDFRSRDKSWDW
                     CRAALGFFRENNIPFHEMACANALIGNSSNDNSKYCLAKPGALYLVYLPDGGRVGLDL
                     GKDRGEFTLHWFNPRQGGPLKTPDGKLSGGTPVTLTAPDENDWLAVLRKL"
     gene            27738..28748
                     /gene="neuB"
                     /locus_tag="PLP58_03755"
     CDS             27738..28748
                     /gene="neuB"
                     /locus_tag="PLP58_03755"
                     /EC_number="2.5.1.56"
                     /inference="COORDINATES: protein motif:HMM:TIGR03569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0016051 - carbohydrate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate synthase"
                     /protein_id="HRJ07671.1"
                     /translation="MPRAFIIAEAGVNHNGDIELARRLVRAAAEAGADAVKFQTFQAA
                     HLVTADAPQAEYQARNTGKTESQYDMLKRLELPLAAFAELAALCRELGIEFMSTAFDE
                     ESADYLAGLGMGVFKIPSGELTNLPFIRRIATYGKPVIVSTGMGTLDEARAAVETIRA
                     AGDDKITVLHCVTNYPAPPDSVNLRAMRTLRDALGVPVGYSDHTEGIEAPVIAVAAGA
                     CVIEKHFTLDKDLPGPDHKASLDPAELAEMVRAIRRAEVLLGRAEKQPGATELEVAKI
                     VRRSVVAARDIPAGMEITEAMLVLRRPGTGIPPAHLHEVPGRRARVDIAAGALIQWED
                     LA"
     gene            28745..29902
                     /gene="neuC"
                     /locus_tag="PLP58_03760"
     CDS             28745..29902
                     /gene="neuC"
                     /locus_tag="PLP58_03760"
                     /EC_number="3.2.1.183"
                     /inference="COORDINATES: protein motif:HMM:TIGR03568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008761 - UDP-N-acetylglucosamine
                     2-epimerase activity [Evidence IEA];
                     GO_process: GO:0006047 - UDP-N-acetylglucosamine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase"
                     /protein_id="HRJ07672.1"
                     /translation="MRSVAVVSAGRSDYSIYLPLLRALDGHAEAELRVLATGMHLEER
                     FGRTVDWIERDGFPVTARIPVLQNADTPQAIAAAMSAGLAGFGGHFERERPDILVVLG
                     DRFEMFAAALAAVPFNLPILHIHGGELSEGAMDDRFRHALTKLSHLHATAAEVYRKRV
                     IQLGEEPWRVHYTGALALDNVRALPRLDRAELEKRFRLDLSEPPLLVTLHPTTNEHEL
                     AGAQTEALLEALRRLDLPVVFTMPNADTAGSVIRERILSFCAEWKKARWVENFGPEGY
                     FSMLREARAMVGNSSSGLLEAPSFALPVVNLGIRQKGRLRAGNVLDAPFEAEAIAEAV
                     RQALTSEFKARTQGHEHFFGDGQAAPRIVDLLLRTPLDARLLDKTFHDLPS"
     gene            29899..30519
                     /locus_tag="PLP58_03765"
     CDS             29899..30519
                     /locus_tag="PLP58_03765"
                     /inference="COORDINATES: protein motif:HMM:TIGR03570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NeuD/PglB/VioB family sugar acetyltransferase"
                     /protein_id="HRJ07673.1"
                     /translation="MKCVLLGAGGHGRVLIEAFQPKTFDGILDARPGLGSVAGVPVLG
                     DDEMLPRLPGLGFTHFVIGLGSTADCSARARLYESAVQAGLSAAEVMHSTAWISPCAT
                     LEAGCQVLARAVVHVSAKLGENVLVNTGAIIEHDCQIAAHCHIATGAILCGGVIVGES
                     AHIGAGAVIRQGIRIGARALVAAGAVVVKDVPDGGRVAGVPARALP"
     gene            complement(30607..31173)
                     /locus_tag="PLP58_03770"
     CDS             complement(30607..31173)
                     /locus_tag="PLP58_03770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ07674.1"
                     /translation="MGEEFDRGGEHVVYRSSHDPDVFFKITHPGQAGLTVVTHQNRHG
                     EYNSAIGSAAPLEYLERMILANEVFQDAIRFEGVIEGDLPSLVISQPKLEGALPEQKE
                     ITLHLRDRLGFDRVDTLIWYRPVDGLVIGDTKRSNFIETPDGQIVPIDLTACFATPEM
                     AESWGYPPPPACDLRDELASLRRPARTA"
     gene            complement(31212..31397)
                     /locus_tag="PLP58_03775"
     CDS             complement(31212..31397)
                     /locus_tag="PLP58_03775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ07675.1"
                     /translation="MQSIPQRPTLEQIQEEQHKMGRARRKWILTDPVKTEQQMKRNRE
                     LRLKIEGPPVLSSSYDW"
     gene            31590..32636
                     /locus_tag="PLP58_03780"
     CDS             31590..32636
                     /locus_tag="PLP58_03780"
                     /inference="COORDINATES: protein motif:HMM:NF012695.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HRJ07676.1"
                     /translation="MSSKPVSQLCLPPDGTLWDALQVIDKGACGIALLVGADQRLLGT
                     VTDGDIRRALLAGASLQDRVEPHARRGFTSVNAVTPRAEVLDLMQARQIHQIPILDDE
                     GRLKGLHLLHEIIGSAPRPNWAVIMAGGKGTRLGELTRATPKPMLKVAGRPILERLVL
                     HLVGSGIRRVFISTNYLAGIIEDHFGDGARFGCAIEYLREEPGEPLGTGGSLALLPER
                     PEQPVFVCNGDLVTQVDVAGMFGFHEEGGHAATIGVRGYSHEIPFGCLDVSEGRVTSI
                     VEKPAISQLINAGIYILSPEVIGRVPRRHFPITELFEEALRLGDSVGAFEIADEWLDV
                     GQREQLKQARGGAH"
     gene            32643..33629
                     /locus_tag="PLP58_03785"
     CDS             32643..33629
                     /locus_tag="PLP58_03785"
                     /inference="COORDINATES: protein motif:HMM:NF027681.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HRJ07677.1"
                     /translation="MGSFENQTVLVTGAGGFIGSHLAEHLVREGAKVRALVHYNALGK
                     RGWLDHSDLAGDMEILAGDITDRDSVRAAVKGCGTVFHLAALIAIPYSYQAPRSYVRA
                     NIEGTLNVMQSALETGARRVLNTSTSEVYGTARFVPITEEHPLQGQSPYSASKIGADK
                     MAEAFAASYELEVVTVRPFNTFGPRQSMRAVIPTIITQCLRGDEVRLGAVTPTRDLNY
                     VQNTVEGFCAAALSPNAAGEVINFGSGREISIGDLAVLIGKMLGKDIRVVCEQERLRP
                     SKSEVERLLACNAKARSLLGWEPRVSLEEGLEKTLAWLQENRAHYQAQGYVV"
     gene            33619..34788
                     /locus_tag="PLP58_03790"
     CDS             33619..34788
                     /locus_tag="PLP58_03790"
                     /inference="COORDINATES: protein motif:HMM:NF013227.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LegC family aminotransferase"
                     /protein_id="HRJ07678.1"
                     /translation="MSSDTPFIPLSVPHLGGNEWAYIKDCLDTGWVSSVGSYVTRFET
                     ALAQAAGTPFAVACNCGTAALHISLLVAGVRPGDEVLVSSMTFIAPVNAIRYANAVPV
                     LVDSEAAHWQMDVEQVRRFLEDGCERREGVLFNRATGRRVTAVIPVHILGHPVDMAAL
                     MPLAERHGLTVIEDATESLGSTLHGRAAGGIGHLGCYSFNGNKIITTGGGGMIVTARE
                     DWARRARHLTTQAKADAEEYIHDEVGYNYRLTNIQAALGCAQMESLPAYVARRRQIQA
                     LYQSGLATVPGLRFQGEAPGVVSNCWLTTVRVDAAAFGMDARSLRARLKAERIEARPL
                     WQPAHLSPAHQDAVHLPCPVAEDLYQNCLSLPSSSSASDADIQRVVAAVKSAAAA"
     gene            34888..35283
                     /locus_tag="PLP58_03795"
     CDS             34888..35283
                     /locus_tag="PLP58_03795"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009959498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HRJ07679.1"
                     /translation="MSDIALAKGQKFLEDNAAKEGVVTTASGLQYKVIRAGEGRSPSA
                     TDTVVVHYRGTLIDGKEFDSSYKRGEPIEFPLNRVIRGWTEGVQLMKEGAEYRFFIPS
                     QLGYGTRGAGRDIGPNEVLIFDVELLEVV"
     gene            35332..36231
                     /gene="lipA"
                     /locus_tag="PLP58_03800"
     CDS             35332..36231
                     /gene="lipA"
                     /locus_tag="PLP58_03800"
                     /EC_number="2.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009962189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016992 - lipoate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009107 - lipoate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl synthase"
                     /protein_id="HRJ07680.1"
                     /translation="MAIKIDPALHKEKKPDWIRVKLPRDPVFWSTKALISDLKLHTVC
                     EEAQCPNRWECWSQGTATFMIAGDRCTRACGFCAVKTAKPFALEADEPQRVAQATKRL
                     GLNHIVITAVARDDVADGGADHFARTIEAVRAEVPGITIEILVPDFNGKDDALEAVMK
                     ARPHIFNHNLETVERLTPLVRSRAQYHRSLHVLKRAKELAAEAGTKCATKSGLMLGLG
                     ETEVELFQAMDDLLEHGVTVLTLGQYLRPSEKHLPVVEYVHPDTFENYKEIARKKGFR
                     HVASAPLVRSSYHAADFKPEQDL"
     gene            complement(36317..39112)
                     /locus_tag="PLP58_03805"
     CDS             complement(36317..39112)
                     /locus_tag="PLP58_03805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4350 domain-containing protein"
                     /protein_id="HRJ07681.1"
                     /translation="MRLEDLTLVLRPRSSWEAADLGCALVRRDYGRILALWAVTVLPV
                     WALLAALLWRWPVAFSLVVWWLKPLYDRVPLHHLARAAFGGKPTLGETLRAWPGLWTR
                     FLFSALLWRRLSLMRSFTLPVLMLEGQRGRAARARVSTLASDGGSGGAMLTWVFVHLE
                     IAAAFGLMALTSTLGPAEGLPDFQEMLLNPEEALAGTHAQQWLGNLLYLVTITLIEPF
                     YAGAGFGLYLNSRIKLEGWDIELAFRRLAARLRPVVTVLLLLFSCLVATAQAATPSGA
                     EPVDKEAVKQKAAEVLARPEFEEHVFKQKTWVPELGGSSGEWDLSFLSQILGPLGYLL
                     VGALVALLVWLVARNAGFFSIRRGARPPVPTPAAPRVLMGMEITPESLPPDIPAAARA
                     AWREGRAREALSLLYRGSLSRLVTQHRLPIRGSDTEDDCLLLAARLGQEGVSTFFRQL
                     TLLWVRAAYAGREARVEEFEHLCAGWPFEKNSPSTARRTPFAPAALLCLLLTACSGKW
                     EDVEIELGHKGKARLDPFLAAQHLLQDLGHEAARQPALKLPPDASGAMLILSAEGGMP
                     EGRARQLLQWTREGGHLVYALAGCAPYNDWGGSGGGLGLALADHSDDPLLRALGVKAT
                     GGVKVGSLVEDLKNLRKDAKQPEKSGDESAEKKDAAAAGEENPPADNDELEAPGAETG
                     EKKKNLVIDLGDMEDMPLKTRAYRQAGQEFEFEMPAVHHFTIKRRLAEGEHAAGTRKE
                     AAALSLRHGQGRVTLLAHARPFRNRYLDEHEHAAWLAALAGNEPRRVWFIVSLNSGFF
                     ELLWSRAWRPLTALGLLVLAWLWMSLPRFGPARAAALHQTRHFADHLAALGQFFHRLR
                     RDDLLLAAAQDAVRVRVLRARPHLAGDDEAQLAWLAAHSGMALEQIRALMTPPEARAR
                     VSSLARRMQDLQALARA"
BASE COUNT         6865 a        13579 c        12192 g         6560 t
ORIGIN      
        1 gctcgccgcc gccgccgcgc ccggcctggc catgacgccc tccctcgcct ggctcgagtg
       61 gggcctgcgc acaccctggg tcaccatccc cgtcgccgtc gccgcctttc tcatcgtctg
      121 gctcagctcc catgccgtca atgtcctcat cgccctctcc cccttcggca tcgtggactc
      181 cgcgttaaag ctcttcaagc tcgccatctt cacactcgtc ttcgccatca ccgccgtcag
      241 cgcctgggcg ggcatcgcct tctgcctgct gctcatcctc ctcgccatgc tcgtggccgg
      301 ctggtccttc cgcctcagcg tcttcggcgc cctgatgggg cgcgacttcc tgctcaacaa
      361 gcacgccacc gccggcgagc tcgatgacgg cgtcaaaggc ttcctctccc gcagcaccgc
      421 cggcgtgccc gcccgcacct acggacgcat ggaaaacgac gccggcggaa gcgccggttt
      481 cgcctaccgg ccctggctca tcctgccgga gaaacgcatc ctcctgcccg ccgaaggccg
      541 cgtgctctgc aagggcgtcc tccaccccgg actgctgcac agggacgcgc cggacgccgc
      601 ggaaaagacc cgcctgcgcg gctgcctcat tctcctgccg cgctaccggg gccttgagga
      661 aagcatcgcc gcccggcacg gctgccagga gatcgtggac agcgccctgc tgcgcggatt
      721 caaagccgtg cgccactggt tcgtcgagat gctcgccctc ggcagaccgc gcgagcgcct
      781 cgcctgaaaa atcccgcgct ttccgcgagg ccgcgcgtaa cctcggcccg cgctgatgtc
      841 ctcgctcttc atctggttcc ggctgctctt caactgccgc ttctactacc ccgtcttcac
      901 gatcctgttc cttgatctcg gcctcagcat cgccgagttc gcagcgctca atgtcgtctg
      961 ggccgtcacc atcgtcctcc tcgaggtgcc atccggcgcg ctggccgacc gctttggccg
     1021 caggcccctc attgtcgccg ccggctggct catggtcgcc gagatggccg tgctctgcct
     1081 catgcccgtc ggggatcacg gcaccgtcct ctggctcttc gtcctcaacc gcatcctcag
     1141 cggcgccgcc gaggcctgcg ccagcggagc ggacgaggcc ctggcctacg actccctccc
     1201 ggagcaggac cgcgccgcgc tctggcccgc agtgctcgcg cgcctcagcc gtgccatggc
     1261 cctcggcttc atcgtctcct ccgtcaccgg cgccgtcatg tatgatcatg ccaaagtcag
     1321 cgccctgctc gcctggctcg gcatggacgc catgccgcgc cccctcaccc tgaagctgcc
     1381 gctgctcatg aacctcctca ccggcgtcgc ctgcctcctc gtcgccctgc gcatgaggga
     1441 gcccgccgcc catgacaccc ggccccgcct gccctggacc gccgagaccg cgcgcgccct
     1501 gcggggcatg ttcggcaccg gcctctggat atggaggcgc cacgacgtct tccgcctcct
     1561 cgtgctcgcc gtcgtcttcg acagcgtcat ccgcctcttc ctcaccgtcg ccagccagta
     1621 ctaccgcctc atcggcatcg gcgaggcatg gtatggagtc atcggcacca ccgccgccct
     1681 gctcggactg gccagcgccg gcatgatgga aaaaatgacc ctgcgttcca ccctgcgcgg
     1741 aaacttcgcc ttcctcgccc tgctcaccct cggcggactc gcctgcgccg ccgccgccgc
     1801 gcccggctgg gccggggtgg ccctcgtcat gcccgtcatg ctcgccatgc gcttcctcca
     1861 gttcttcctt tcccactacc tcaacgcgga aaccggctcc gaccgccgcg ccaccgtgct
     1921 cagcttccgc ggcctcgcca ccaacctcgc ctacggcggc atgaccctgc tcttcggctg
     1981 gcacaccggc tggctcaaaa acgcccgcga accgcccgcg gacgacctca tcgtcttcgc
     2041 ggacagcttg aaactctggc ccgcctggtt cgccgccacc ctcctgctct ccctcctcct
     2101 catcacctgg cactcccgcc gctgagagct gacagacggc cgggagcgac tggggatcga
     2161 aggggtggcg cgggggcgcg gctcccggga gagcactcgc tatcgccccg cactcctggg
     2221 cgcatcaccc aagcccgtcg ggcttggaag ggttgcctgt ccgagttgtt gaacccatcc
     2281 agcagcttcc aactcccgtt gaatgcccct gaagcgctct tcaactctgt ttatgtaacg
     2341 atgctggttg attcccctca tcccgcccgc cgtcgcctga gccaaagcat gggaagtcat
     2401 gaaacagctc tgcatccctt tcatcatcgt ctccagcatg gttgcggcaa ccttggcact
     2461 gcacgcccac gaagcaccgg tattgtggcc gggatgcgcg cgctctcacc ttgggccaga
     2521 gcggcggcgc ctggtgtgcg agcgcggaca tacgcaccct gccagcctcc cggcagcgac
     2581 acgctgctcc gcgtccaaaa aattttcacg atcctggatt gtcaaaatcc gcagaatgat
     2641 ttattattga aattatggaa gttgtaaaag agccaggccg cccccttctc ctcttttgtc
     2701 tcggcacccg gccggagatg atcaagctcg cgccggtcat cctgctggcg aagcagcgcg
     2761 gcatggacac cctgctggta aacaccggcc agcacgcgga cctgctttcg cctttattcg
     2821 acctcttcgg gctgcatccc gaccatgacc tgacggtgat gtgcgcgaac cagacgctca
     2881 acggcctgct ggcgcgcatc ctcgaaaggc tggaccccat tctcgaaaaa gccgcgccga
     2941 gccatgtcat cgtgcaggga gacacggcca cggcgctggc cggcgcgctg gcggctttca
     3001 accgcaaaat ccccgtggcg catgtcgagg cggggctgcg ctccggcaac cgcctgagtc
     3061 ccttcccgga ggaaatgaac cgccgcctgg tcacggagat tgcggacctg cacttcgccg
     3121 ccacgacgct gaaccgggac actctgctgg ccgggggcgt gcccgcggag cgcatccaca
     3181 ccacgggcaa cagcgtggtg gacgcgctgc accacaccct ggcgcggagc aagccgggca
     3241 ttgacctgct ggcgctgcgc cggcaggtgg cgggccgccg cgtggtgctg ctgaccacgc
     3301 accggcggga aaacttcggc gacaccatgc gcgcctattt gcgctcgctg cgccgttttg
     3361 tggaggcgca cccggacctg tgcgtggtct tccccatgca cccgaacccg aatgtgcgcg
     3421 ccgccgccga ggcggagctg ggcgggtgcg accaggtcat catcaccagc ccgctcggct
     3481 atgccgactt cgtgcatttg ctcaacgacg cctggctcat cgtctccgac tccggcggca
     3541 tccaggagga ggcatccgcg ctgggcaagc cgatcctcgt tttgcgcgag aacacggagc
     3601 gccccgaagg cgtgaatgcc ggagtggcgc ggctcgcagg cgagcaaccc gaccagctcg
     3661 accatctgct gggcggggct gtggcggaca aagcctggtt cgagcaggcc gcgaaggctg
     3721 aaaaagtgta tggcgacggc cgagccgccg cgcgcatcct ggatgtgctg gccggcccag
     3781 cgcccgtcaa ggccgctccg gcccactccc ccgccctgct gcatggattc gccgccgccg
     3841 cccccctcct gcaagcctca tgaacgaccg cgccgccagt gtctccatag acctcgacaa
     3901 ccaatgggcc tacatgaaga cccagggctg cgcgggctgg gaagagtttc ccagctatct
     3961 ggaaaaggtg gcgccgcgca tcctggagac gctgcgcctt tgcggcctgc gggcgacttt
     4021 tttcatcgtc ggcaaggacg ccgcgctgga gaaaaaccgc gcccccctgc gcgccatcgc
     4081 cgacgccggg cacgagatcg ccaatcactc cttcctgcac gagccgtggc tgcatctcta
     4141 tgatgacacg gaactgcggc gggactttaa ccaggcggag gaaagcatcg ccgccgccac
     4201 aggggcgcgc ccgcagggct tccgcggacc cggcttcagc acctcgcccg ccgtgcgcgc
     4261 cttgctgcgg gagcgcggtt atgtgtatga cgcctccatc ctgcccacct tcatcggcgg
     4321 actggcgcgc gcatatttct tcatgacctc gaagctgccc gccgaggaaa aaaagcgccg
     4381 ctcccggctt tatggacgtt tcagcgacgg cttcacaccc ctgcgcccct acgccattga
     4441 gccgggactc atcgaggtgc ccgtgaccac cctgccgctg ctgcgcacgc ccattcattt
     4501 gagctacctg cttttcctcg gccaatacgc catgcccctg gcccatgcct actggcacag
     4561 cgccgtggcc gcctgccgcg ccgccggagt cggcccctcc ctgctgctgc accccacgga
     4621 tttcctggat gctgacgatg tgcccgccat gcgctttttc cccgccatgc gcctgccctc
     4681 ggcgcgcaaa atcgccctcg tgcgcctcac gctggaaagc ctgcgcgcgc actggtccac
     4741 cggcaccctg ctgcgccacg cgcagagcct ggccggagaa aacaccgcct gtctgcaaaa
     4801 agtccaagcc tgttaatccc gcacccgaca cacccaccat gagcaccgcc gcatccatcc
     4861 aggaagtcgc cgtcgtcctg ccggccttca acgaggagaa agacctcccc aacctgctcg
     4921 cgcgcatccg cgagaccctc accgcccagc ctttcaaata ccaggtcatc gtcgtggacg
     4981 acggctcggc ggaccgcacc gccgagatcg cccgcgaggc cgccgccgcc atgccgctgc
     5041 gcctcatcca gcatgagaaa aacagcggcc ttggcatggc catccagacc ggactgtccg
     5101 ccgccatgaa atgcgcggac atggtcgtgg tcatggactc ggacaacacg catgatccca
     5161 tccacgtcat ggaaatggtg gccgagatgg aaggcgggga caccgatgtc gtcatcgcct
     5221 cccgctttca gcccggcagc gtcgtcatcg gcgtgcccgc ctaccgccag ctcctgagca
     5281 tcggctgttt cctgctcatg aaagtgctcg tgccctaccg cggcgtgcgg gactactcca
     5341 ccggcttccg cggctaccgc gccggcatcc tccagcgcct ggccgccgcg cacggggagg
     5401 acaagctggt ggaggaaagc ggcttcgtct gcatgcttga ggtgctgctc aagctgcgcg
     5461 ccctcggcgc ccgcgcctgc gagatcccct acaccctgcg ttatgacttg aaggccggcg
     5521 ccagcaagct gcgcatctgg cgcaccttga aacgctacct cgccgtcgtg gcccgccacc
     5581 gctccagcaa aaccgagccc gccctgatga aggtggccga agcacgcgcc taaccctcga
     5641 ccaaagccgc agccgctgtt tgaactccga acgcaccctc atcctcggcg gcggcatcag
     5701 cgggctcgcc tgcgctttgc ggctggccgg ggccgggcgg aaagtcacgc ttgtcgaagg
     5761 ctccgaccgc ctcggcggac tgggcgcctt cttcgagcac gaagggcgca ccttcgaaaa
     5821 attctaccac tgcatgctgc cgagcgacgg gccgctgctc gcgctgctcg actcgctcgg
     5881 actgaccccg gagatctact ggaagccgag caccttcgcc tatgccggag cggggcgcat
     5941 cttcccgctg aacacgcctt tggatctcct ccgcttcgcc cccctgccct ttgccgaccg
     6001 catccgcgtg ggcctcaccg gactctgggg ccggctttgc tcggacaagg gactcgacga
     6061 cctcagcacc gtggactggc tgacccgcct ctccggccgg cgcgcctttg agaccttttg
     6121 gaaacccatg ctggaagcca agttcggcga ccgccaccag gacgtgcccg cgctttggtt
     6181 ctggacgcgc ttcaaccgcg agaaaggcga gagcaagggg gaggtgaaag gctacatccg
     6241 cgggggttac aaacgcatca tcgagcgcgc cgccgaaaga ctgcgcgaac tcggcgtgga
     6301 gatccgcctg ggcgagtcca tccaaagcgc cgacctggat gaaaacggca gcccctttgt
     6361 catcaccgcc cgcggccgag aggacgccgc gcgcctgctg atcaccctgc cctggccggg
     6421 cctcgcgcaa gtcaccggcc cgcgcctgcg ccaggctgcg caagtgccgg actggggcat
     6481 tgatttccag ggcgtggtca atcacctgct cttcctcaaa cgcccgctca ctccacatta
     6541 ctggctggcc accccggagt cgcagttccc ctttgacggc gtcgtcgaaa cctccgcgct
     6601 cactgacgag gctgaccggg gcacggggcg gcatgtcgtt tacctcacca aatatctgca
     6661 ccgggacgac ccgcgcttcg cccagcctgc ggaggaaatc caccggccat ggtttgccgc
     6721 cttgcagcgc cttttccagg acctcaccga gtccgatgtc gaggccgcgc acaccttccg
     6781 cgccccgtat gtggagccgg tttacacgcg cggcttcctc aagcgccgcc cgcccgaggt
     6841 gctcgtgccg gggaaagtct tcctcgccac caccgcgcag gtctatcccg tcgtcacctc
     6901 ctggaacggc agcgtcatcc aggccggacg caccgtggat gccatgctcc tgtagccgga
     6961 atcacccagt ccgcctcccc tttccctacc tcccttaacc ctgttgcgcc acgcgcggct
     7021 ccgggatgtg gcgcaggatc cagatggaaa gcagggacat gcccacggtg gtcagcgcca
     7081 cggcctgaaa ggagccggtg aagtggtaaa tctggccgct gatgaaggtg gccagcagaa
     7141 gggcgaagac attgcaaaac cccagcaggg cctggagcgt ggagcggcgc tccagcgggc
     7201 agagttcggc ggccagcgtc agatccccca cgcggtcgag gaagaggccg aaaccgagcg
     7261 cgaagtaggc cagcaggaag ccgccaaact gcccggtgaa cgacacccac aaggccaggg
     7321 cgatgcacac aacgcgcgag gcctgcagca gcaccttgcc gccgcttcga tagcccacat
     7381 aggccgccag caggctgccg agcacggtgc ccgcagcctg cacggtgaca aagcgcccct
     7441 cgtcagcctc cggcctgccg gtgacatgca gcgcgtggat ggtgaggaag gagaccatca
     7501 tcagataccc cattccggtg aaacgcgcga gcaccaggcg cacgaggcgg ggctgcgcgc
     7561 gcagcatggc gggcaagccg cgcaaatagg ccaggtaagg gccggctggc ggcgcgggat
     7621 ggcggtgccg ggtgtgctcg tcctcatgca tgaagagctg ggaaagccac gagacaaaca
     7681 gcagcgcgaa ggccgccagg tgcagccacg cataagcctc ccgacccgga taatgcgtga
     7741 tgacgtggtg gatgaccgag ccggccagca cgccgatgac ggcctgaatg atgtaacgca
     7801 cggcccagcc cgcggcgcgc gccttctccg gcaccatgcg cgtgaccatc tccatccacg
     7861 ccaccaccgt cacgccgccg agcaggccgg agaccacggg tgtcagcacg acgatggcga
     7921 gcagcgcgcc gtccacgcgg tcggcgaaca tgaggatgag gcccgtcacc aggtagggca
     7981 gcctttgcag caggccgaaa aacagcaccc acggcttgaa gcgcggcagc ttctccacca
     8041 gcggggcaat gaagatgccg gcgatggcaa acgcggccgg cagcagcgcg ggcatcatgg
     8101 cgatgatcca cgcgcggccg ccgagcatct ccaccatctt cggcagcacg ctctccggcg
     8161 ccagaaacgc ggtgccaccc atgtacaagc cgccctccag gcagtggcag atgaaattcc
     8221 gccgcaccgc gtgggtgtga ggatggtggc tggcggacat ggggcgtggc gcgcggcgag
     8281 tcaggccggc gcgcgcacct gccgcagcca gtcttcgagg ttgtagtagt tcgtcacgcg
     8341 cgtgatgcgg ccgccggcga tgtcgaaaaa cgcgccgacc cgcaggcggt agttttgacc
     8401 catggcgggc ggcagccccg cctcgccctc cagataggcg ccgaggatga agaactcggc
     8461 cgccgcgcgc gtgcccgcgg cattggccat gacttccagt tccacgacct gctcgcggta
     8521 gcagcggtcc atgcgctgga ggaaggcgcg gaaggcggcc ttgccagtct cggagccgcc
     8581 ctggttgatg tcgtggacga cgttctcgga aagcaggtcc agcatcgccg cgcggtcgcc
     8641 ggagttgaag gtggaatagt attcgccgac gagctggagc gcggcttcct gtgggttggg
     8701 catggcccgc atgttagaag gcgcggtgga tgcgtcaacg gcgggcggtg agaggcgggc
     8761 ggcggacgga attcgatttt cgatttttcc cccacccccc cttgtccccg ccgcgcgttt
     8821 ctgctatgcg cacgggatga ctcatccact cttcgatctc accaacaggg cagccctggt
     8881 taccggcggc agcaaaggac tcggcaaggc catggcgcgc ggtttcgcgg aggcgggcgc
     8941 ggatgttttc atctccagcc gccacgagga cgagctgaag gccgccgccg ccgagatcgg
     9001 tgaggggctg agtgtgaaag tcgagtggat ggtggccgac atgtgcgacc gcccgcaggt
     9061 cagggcgctg gctgacgagg ctgtgaagcg gctcggcaag gtggacatcc tggtcaacaa
     9121 cgccggctcc aaccagccgc aggcgattga cgagatcacg gacgaggcct gggaccgcat
     9181 cgtggagctg gacctcacaa gctgcatggc gctgacacgt tatctggtgc ccgggatgaa
     9241 ggagcggaaa tggggccgcg tgatccacat ctcctccgtg ctcggcgtcg gctccaagga
     9301 gaagcgcaac gtctattccg cctgcaaagc cggcctcatc ggcctcgcca aagccagcgc
     9361 cattgacctc ggcgcccaca acatcaccgt caactgcctc tgcccggggc ccttcctcac
     9421 cgacctgcca ggctccctgc tcagcccggc ggagaagcaa gccttcgccg accgcaccgc
     9481 gctcaaacgc tggggccacc cgcgcgagct ggccggcccc gccctcatgc tcgccagcga
     9541 ggcgggcagc tacatcaccg gcgaggcgct gctggtggac ggcggcgctt tcgcgcgggc
     9601 attgtgagcg cgttgtgaca ttgtgaacca ccagcttttc ccgcgaacca tgcgcctcct
     9661 tctttgcctg ccgcttatct tttccatcgt ccactccgcc gaggtggcgg tggcgaatgt
     9721 ggatgaactc aatgccgcgc tgaggcaagc ccggcccggc gacactctcg tgctcgcgga
     9781 gggggagtgg cgggacacgg tcatccgctg caacgcgcag ggcaccgagt ccatgccggt
     9841 cacgctgcgc gccgccgtgc ctggaaagac ggtgctcacc ggccagtccg cgcttcgcct
     9901 tggcgggcgg catgtcgtgg tggaggggct gtggtttcgc aatcccgacc ccgccgcggg
     9961 cgacaccgtc gaatttcggc tcgactcgaa gaacctggcg cggcactgcc gcgtcacgaa
    10021 ctgcgccatc accatggatg cgggccagag cgcgcgtgat gacaaggaaa gccgctgggt
    10081 cggcatttac ggcggtcaca accggctgga tcattgcctc attgagggaa aagtgaccaa
    10141 gggcgccagc gtcgtcgtct ggctcggcga gggccaggag gccgcgcaca ggctcgatca
    10201 caaccacttc ggcccgcgcg agcgcctcgg caaaaacggc ggcgagaccc tgcgcgtcgg
    10261 cgacagcaag acctccatgt ccgccgcctc gtgcatcgtc gaggacaacc tcttcgagcg
    10321 ctgcaatggc gaggccgagt gcgtctccaa caaatcctgc ggcaacacct accggcgcaa
    10381 ttttttcctc gaagtcagcg gcaccctcac actgcgccac ggcaacgcct gcctcgtcga
    10441 ggccaacacc ttcctcggca acggcgccaa tggcacgggc ggcgtgcgca tcatcgggga
    10501 ggaccacgtc gtgcgcggca accactttga acagctcgcc ggcgacgacg cgcgctgcgc
    10561 catcgtcatc atgcagggcg tgcccggctc cgcgctgaac gagtatttcc aggtcaagcg
    10621 cgcccgcgtc gagtccaaca cccttctcga ctgcgagcac cccatcctca tcggtctcaa
    10681 ggacggccgg ggcagcctgc cgccactgga ctgttccttc accagcaacc gcgtccttgc
    10741 gccaaagtcc gcagtcgtcg aggcgcggtg cgacatctcg ggcgtgcgct ggcagggcaa
    10801 tgtcttccac ggcatagccc ccggcatcag cgtgagcgag ggcattgagt ggcagccccc
    10861 ctccatcgct tccgccggcg agccggagcg ccggggctat ggacccgcct ggcggcgttg
    10921 aatggcatgg ccgcggcggc aaccgcgcaa gatggcctgg atgcccgcgt tcccaggcat
    10981 cttcatgaaa aacgcacgtt ctcttgccgc cgcctttatt ttccccctct gcgccgcttc
    11041 cgtcctgcgg gcggacagcg ccggatacaa gccggagttg tttgagagtg tgaggctcgt
    11101 ctatgaggac agcttcgacg gcgcgctgaa caccgagttc tgggaggtgc gccaaagcag
    11161 cacctggaaa gtggcggacg gcgtgctcac aggcagccag tcctcaaagg agtttcagga
    11221 gaaaaagatc gccgagggcg acaaggcgca cgcgggattc aaaccggtca tctggctcaa
    11281 gcaggtgccg gagaacttcg tctgcgtcat gcgcatgcgc tacgacgcgg aggcctggca
    11341 gccgcgcttt ccgctcctcg acatcggcca ccacatacac acgctcgtct tttccgaaaa
    11401 gcagaccacc ctggcgatca aaaaaaacgc cgagatagtc gcggttgagt cccccttgtt
    11461 ttccctcaac gaatggcatg acgtggccat cgaagtaagg aaaggccgcc tcctgctggt
    11521 gattgacggt aaaaagcgcc tgtttgaaag cccgcacata gacatgaccg gccacgccca
    11581 aattgacttc aagggcgtgg acttcggcgc ctgccggata gaccgcatcc agctctggga
    11641 agggcgctga tccagggcgg acctcaccgt ttcctcggcg gcggcttcct gccggggaaa
    11701 agcgtccggc acatctcgca gcgcgtgccg tcgcacccgc gcgcggtgca gtgctcgcgc
    11761 ccgtaaaaga tgatctgaag gtgcagcttg ttccaggcgg actgcgggaa gaggcttttc
    11821 agatcgcgct cggtctgcgt gacgttcctg ccggaggaga gcccccagcg cgccgccagg
    11881 cggtggatgt gcgtgtccac ggggaaggag ggcacgccga aggcctgcgc catcaccacc
    11941 tgcgcggtct tgtggcccac gccgggcagc ctctccaggc tctccagaca tgcgggcacc
    12001 cggccgccgt gctcagccac gaggatttcc gagagctttt ggatggcctt tgacttctgc
    12061 ggccccagtc cgcaggaacg cacgactttt tcaatctccg ccacctccgc gcgcgccagt
    12121 ttctccggcg tgtcgcccag ggcaaagagc gccggcgtca cctgattcac gcgcgcgtcc
    12181 gtgcattgcg cggagagcag caccgccacc agcagggtga aggcatcctt gtggtccagc
    12241 ggcaccggag tctccgggta caactcctca aggcgggtca tgacatgccg tgcgcgctgg
    12301 gccttggtca cgggcggggc gcgttagctg ctgcggccga tgcggtccac catgcgcttg
    12361 acatgcgggt gatgggcgcg cggtttcatg atggcgaggc ttttggaaaa ggacccccat
    12421 ttgagcacgc ggatgttcac attgcgcacg ccccaggcgt tcatgtgcgc ccgcgtcggc
    12481 tggtagatgt ctatggtgtt cgtgcccacc agcgccgagc cgtagtcgtc cacctgatac
    12541 aggtatgggc tgccctcgat ctggaaaatg gtccccaccg ggtaaaccga ccagtccgcc
    12601 gccgcgctgc gcaccgtgcc gtggcgcaga ccggtgccca ccgccgtgcg cgctccgtat
    12661 tggatgtggt ccgactccgt gtgtgtgtag gccgtggtgc gcaccgaggt gatgcgcgtg
    12721 cccttggtcg tcacgatctt gcttttgccg gcgttggagg aggcgcagga gcagagagcc
    12781 agcgcggcca gcaaacacag cagggggagg gtggagactc tcatcattct tgggttatgc
    12841 ggggcaccgg cgtggccggg atttgttgag tctggtcatt tcggcgggga aatcaatccc
    12901 ggatttccac gcgccccgcc cccctgtcac tgcggcccgc gcagaaactc cgtcacgcgc
    12961 ccgccgcgga agctgacgct cgccgccttg taaggcacat aggtgaccat ggagccgtag
    13021 ccgccccaga aggggtccca catgccgccg tagccccagc cgcgacccca gccgcgtccg
    13081 ccccagcccc agcccatgtt ccaagtgtct gtataaacag gctgcgcgcc cagataagtc
    13141 cagcgctccg tctgcgcgcc gcgctgggag ccggaggcca cctggtcggg cctgccccag
    13201 gagagaaaga cagcctcccg cgtcatgccc tcgcgcaggc ggccctgctg gacgaggagc
    13261 ttgtccgtct cgctcagtct ggcgtagatt tccgggttcc gctggatccg cttctgcacg
    13321 gggctggcgc attgggtgaa aatgagggct gcggtgaggc agagggcggc gcgcaacagg
    13381 gctggggctt tcatgacggg gtgttatttt gtcaattcac agcggacagg cacggcggct
    13441 gtggcgttca atggggaaaa tcacagccgc atggcaaaat gcacctgccg gccgatctcc
    13501 tcgaagccgc aggaggggta aagactctgg ccgatcacat tcgatgccat cgtctcaatc
    13561 ttcgccgcct gcagcccctg ctcgcggaaa tagtccaggg cgtggtggat cagccgccgg
    13621 cccaggccga gaccgcgcgc cgcctccacc accgccagat tcgggatgcg gccgattgag
    13681 ttcacgcggt ccacccgcgt cgtgatgtag ccgagcacct ccccctggcg ctcggccacg
    13741 aagcagcctt ccgggttcac cgtgcagtcc tcgtcaatat ggtccgcctt ccgcgccttc
    13801 cagtcgcgcc cgttccacac ccccaggcgg tcctccagca tctggtcgag tgccacggag
    13861 ccaaacgaat ccaccgtgat gcggcggaga atttccagat cctcgggacg ataatggcgg
    13921 atggagatgg gggcggcgtc gctcatgggg atttaatatg gccagcggta gggcgcgttg
    13981 tggatgagtt tgttcttggg atacgcgctg atgcgcacgc tgtttttcga gttcccgctc
    14041 agcagccaga cattcgtggc atccatcgcg tgcaggaagc cgatgtgccc gctcgtcgcc
    14101 tggtcgctgc ccagcggctg caaaatgcac accgcgccgt aagtcgccgc cacgggccgg
    14161 ccccagcccc accagtccga gacgcgcccg ctccgcgctc cgcggaagcc ggcctgctcc
    14221 aggcaccagt tcacaaaagc cgcgcaccac gccgtctcat ccggcttgtt gggcagtccc
    14281 acgctcgcca gatattgctg gatgcgcgga ttgtcccctg gctgccattc ctgcacgccc
    14341 acctccatga tggcgatctg catccacggc ggcggcgccg caaagccagc cccgcctgcc
    14401 gcgcccggct gcgcgccctc cgcaggcagg cccgtcaccg tgcctgacac ctgcaccacc
    14461 tggccgggga aaatgacccg gcactcgttg ctcccgaaag tacacagtcc gggattgagc
    14521 cgctgaaact gagccagtgt cagtccgaac tttttggcga tcttcgacgg attgtccccc
    14581 ggctgcacga tgtattgcga catgccgcca gcctacctct ccccgccagc gtaaagcaac
    14641 gccgaagttc gaaattcgaa gctcgaaatt cgaaattcaa accgcacatt ccgaaatccg
    14701 cactcccgat gcccgacgct ctcacactcc acctcgttca gcttgactcc caatgggagg
    14761 acaaagcggc gaacttcgcc cgcgtccgcg acctcatcgc ccgcgcaatg cccgctcccg
    14821 gcggcctgat catcctgccg gaaatgttcg ccaccggatt cagcgaccgc ctcgccgcca
    14881 ccctcgagcc cctcgacggc cccgtcaccg cttttctccg cgacatcgcc tcccgcaccc
    14941 gctgcgccgt cgtcggcggc ctcgtcaccc cggcgccgga cgggcgtgga ttcaatcaag
    15001 cactcgccat cgccccggaa ggctccgcac tcgcccgcta cagcaagacc catcccttcg
    15061 cccttggcgg cgaggattcc gtccacgcgc ccggcggtcc gcccgtcatc ttcgagtggg
    15121 gcggattgaa aatctccccc ctcatctgtt acgacctgcg cttccccgag accgcccgct
    15181 ccgcgctcgc cctcggtgcc gaggccctcc tctacatcgc ctcctggccc gttcgccgcc
    15241 aggaccactg gatcaccctg ctccaggcgc gcgccattga aaaccaggcc tatgtcatcg
    15301 gcgtcaaccg ctgcggcaac gacccgcgcc acagccacgg cgggcgctcc ctcatcgccg
    15361 acccgcacgg cgtcatcatc gctgacgccg gagcgcagga gcgcgtcatc accgcgcgtg
    15421 tcgagcccgc actcgtccgc gaatggcgcg cgcagttccc cgccgcgcgc gagtatctcg
    15481 cccttcagcc ttgaaagcgc cgtttccgga tcatgccgcc agacccgcca ccacccgaaa
    15541 cagcggacac cgcgcccggc agcctttgca ccgcctgcgg cctttgctgt gacggcaccc
    15601 ttttccacat cgtccgcctc cagcccgcgg attccgcccg ccagttgtca gccctcggca
    15661 tgaagctgcg ccgccgaaaa aaagacccct acttcaccca gccctgcgcc tttctgcacg
    15721 actgccgctg cgccatttac gagcaccgcc cccagcgctg ccgtcacttc gagtgccgcg
    15781 tccttcagcg gctgcaaaac gacgagatca cccctgttgc cgcgcatgcc ctcatacgcc
    15841 aggcccgcca gatcgcgtgt ggcattgagg ttcagctcgc ctccggagac gacaccgccg
    15901 cgcatctccc cctgcttgag cgctgcgagg ccgccctggc cgccatccca tccggcactc
    15961 cggccagttc gaatttgagc gccgctctcc aggaacttca gcgcctcctg cgcgccgagt
    16021 tccacgctct cggttgagtt ttccagtcct ctgcgcccct gacgccaaac gcgcgtccgt
    16081 cttgaccaaa atatgggagg catcctggct ggacactggc atgaaagcag cgtctttgac
    16141 aggcacactc ccgcacaccc atgagcaaga caaatccctc caaaccactc agcaaaaaca
    16201 tccacctcct ccgctgggtg aagaagatgg ccgccctgtg caagcccgac cgcatccact
    16261 gggtggacgg ctccaaagcc gagtatgacc gcctctgcga gcagatggtg gaaggcggca
    16321 ccttcatcaa gctcaaccag aaacagtggc ccggctgcta ttacgccaag tccgacccca
    16381 gcgacgtcgc ccgcgtggag gagcgcacct tcatctgctc ccactccaag gacggcgcgg
    16441 ggcccaccaa caactgggag gaccccttcg agatgaagaa gaagctgcgc ggcctcttca
    16501 acggctgcat gaggggccgc accatgtatg tgctgccctt cagcatgggg ccgctggact
    16561 cgcccatgtc ccagatcggc gtgcagctca ccgactcgcc ctacgtcgtc gtgaacatgc
    16621 gcatcatggc ccgcatcggg aagaaggtct tccagatcat agacaagggc gaaaagcgcg
    16681 tcgtcccctg catgcacacc gtcggcgcgc ccctcaaaaa gggccagaag gacgtgtcat
    16741 ggccctgcaa caaggagaaa tacatcgtcc acttccccga gacgcgcgag atctggagct
    16801 acggctccgg ctacggcggc aatgccctgc tcggcaagaa gtgcttcgcc ctgcgcatcg
    16861 cctccgccat ggcccgggac gagggctgga tggccgagca catgctcatc ctcggcgtcg
    16921 agaaccccaa gggcgagaag acctacgtcg ccgccgcctt cccctccgcc tgcggcaaga
    16981 ccaacttcgc catgctcatc ccgcccgccc acttccagaa gcagggctgg aaagtctgga
    17041 ccgtcggcga cgacatcgcc tggctcaagc ccggccccga cggccgcctc tacgccatca
    17101 atcccgaggc cggcttcttc ggcgtcgccc ccggcaccag cgaccacacg aaccccaacg
    17161 ccatggccgc cctgcggaaa aacaccatct tcaccaacgt cgccctcacc cccgacggcg
    17221 gcgtctggtg ggagggcatg accgatgagc cgcccgccaa atgcaccgac tggcagggca
    17281 accagtggac gcccgagatc gccagggaaa aaggcgtcaa agccgcccat ccaaactccc
    17341 gcttcaccgc ccccgccgcc cagtgcccca ccattgaccc ggactgggag aagcccgagg
    17401 gcgtgcccat ctccgccgtc atcttcggcg gccgccgcgc caccaccatg cccctcgtct
    17461 atcagtcctt caactggagt tgcggcgtgt acatcggcgc caccatgggc agcgagatga
    17521 ccgccgccgc cgccggcacc gtcggcaagg tgcgccgcga ccccatggcc atgctgccct
    17581 tctgcggcta caacatgggc gactacttcc gccactggat ctccatgcag cgccgcctca
    17641 ccgagacacc ccgcgtcttc aacgtgaact ggttccgcaa ggacaaggac ggcaagttcc
    17701 tctggcccgg atttggtgaa aacatgcggg tgctcaaatg gatcgtggac cgctgcacag
    17761 gccgcggcat cgccaaggaa acccccgccg gctggatgcc ccattacgag gacatggact
    17821 ggaccggcct cgacttcccg aaggagaagt tcgacgagat ccagcgcttc gaccgcgacg
    17881 cctggcgcca ggagatcctc ctccacgagg agctcttcct cgacctccgc agccacctgc
    17941 ccaaggagat gatctacgag cgcgagctgc tcatctgccg catgtgaagc tttgcacggc
    18001 agcttggaga taatcgtgcc cttgggggac gcgaaacttg atccaaggtt tgcactccag
    18061 cccgctcgca ttcgcccggg ggatgaagct gacggtttca caaatcgtgc agacgcgcgg
    18121 cttaaatccg cgcgctcgct tgcgcgcagc ccgcgtatct ccaggttcac gcccccatgc
    18181 cgaaaaaagc tcccgtcaaa gccaagcccg ccgcccccgc cgatgtcatc caggtgcgcg
    18241 gcgcgcgcca gaacaacctg cgcggcattg atctcgatct gcctcttggc aaactctgcg
    18301 tcgtcaccgg tcccagcgga tcgggcaagt cgtcgctcgc ttttgacacg atctatgccg
    18361 agggccagcg ccgctatgtg gagacctttt ccccctacac gcggcagttc tttgagcgca
    18421 tggacaagcc gaaggtggac gccatccacg gcatcccgcc cgccatcgcc atcgagcagg
    18481 tgaacaacat ccgctccacg cgcagcaccg tcggcaccat caccgagatc aatgactacc
    18541 tgaaaatgct ctttccgcgc gtggctgagg cgagctgtcc cgaatgcggc aagcccgtgc
    18601 gcccggagtc ggcggagagc atcctgcaca cgctgctgca tcagcactca ggcgcgcagg
    18661 ctctcatcag cttcggcgtg ccggtgccaa agggcaccaa gccggaggac tttttcccct
    18721 tcctgcaaag ccaaggctac ctgcgcgtgt ggctctacgg cgaggtcctg cgcacggatg
    18781 aacccgcgtc tttgaaaggc aaaaagctgc ccgccatcgt gctcgtcatc caggaccgcg
    18841 tcacgctcac cctcgaaaac aaagcgagaa tcagcgaggc tctcgaagca gcgctacgac
    18901 tgggcaaagg caccaccacc atcgtcttcc cggaaaatcc gcaatccgca atccgcagtc
    18961 cgcaatcctt ctccaccgac tggcgctgcg ccgactgcga cctgaagctg cccgccccca
    19021 cccccggcct gttcagcttc aacagcccca tcggcgcctg ccccgcgtgc aagggctttg
    19081 gccgcacgct cgggctcgac atccacaagg ccatgccgga cgaatccctc agcatcagcg
    19141 aggggctcgt gcgggccttt ggcggcagca cctacagcga gagccaggac gacctcgaac
    19201 gctgcgcccg caagcgcggg gtggacctcg acacaccctt ttgcgacctg cccgccgatc
    19261 agcaaaagtg gatcatcgaa ggcgaggact ccgatcccga gcgcgcctgg aagcgcggcc
    19321 tctggtatgg cgtgaagggc ttcttcaaat ggctcgaagc ccgcgcctac aagatgcatg
    19381 tgcgcgtctt cctcagccgc ttccgcgcct acaccacctg cggggactgc gatggcgggc
    19441 gcttgaaaaa ggaggcctac aacttccgcg ccaacgggcg caccatcgcc gacctgtgga
    19501 acctgcccgt caccgatttg ctgcccttca ttcaaagctg gcccatcacc gggagtgaca
    19561 gcgccgcgct catgctgcgg gatgaaatct gcgcccgcct caattacctc gaccgcgccg
    19621 gcctcggcta catccatctc gaccgccaga cgcgcacgct gagcggcggc gagctccagc
    19681 gcgtgaacct caccacctgc ctcggcgctt ccttggtgaa cacgctcttc gtgctcgacg
    19741 agcccagcat cggactgcat ccgcgcgaca tcggccgcct catcggcgtc atggagggat
    19801 tgcgcgacaa gggcaacacg ctgctcgtcg tcgagcatga ggaggcggtc atgcgcgccg
    19861 cggaccacgt catcgagatc ggccccggtc gtggcgacaa aggcggcacg ctcgtctatg
    19921 acggccctct cgccggcttg gccgaggcga aagactccct caccgccgcc tacctgaccg
    19981 gcagcaaaac catcccgctt ccagagaagc gccgtcagat ttcgaatttc gaatttcgag
    20041 cttcaccccc gtcgaagccc gcaaagtcca agtccaaaaa aaccgccccg ccagccttca
    20101 accccggctt cctctccatc gtcggcgcgc gccagaacaa cctcaaaaat ctcgacgtgg
    20161 acatcccgct cggtgtcttc tgctgcatca ccggcgtctc cggctccgga aaaagcacgc
    20221 tcatccatga ggtgctttac cggaaccttc agcgcctgcg cggcgaggtc tgcgaggacg
    20281 agccgggccg cgtgcgcagc atcctcgggc acgagcagct cagcgaggtg gtcatggtgg
    20341 accagtcccc cctggcccgc accccgcgca gcacgcctgc ggtctatgtg ggcgccttcg
    20401 ataccttgcg cgcgcttttt gccgagagcg aggaaggccg cgcgctcggc atcacgcccg
    20461 gcttcttctc cttcaactct ggcgaaggcc gctgtgagcg ctgcgccggc acgggctatg
    20521 aaaagatcga gatgcagttt ctcagcgatc tctttgtcac ctgccccgag tgccagggca
    20581 cccgctacca gccgcacgcg ctcaagatca agctcgacgg caaaagcatc cacgacgccc
    20641 tggagatgac gctcgatgaa gccgtggcgt ggtgtaggtc aggcgtctcg cctgacccgt
    20701 gtgaaccggg catcctgccc ggttccaaaa cccagtccaa gcgtttcgcc caactcgcca
    20761 ggcagctctc catcctctgc gaagcggggc tcggttatct caagctcggc cagccgctca
    20821 acaccctctc cggcggtgaa gcgcaacgtt tgaaactggt ggggcatctt ttgaactcgc
    20881 cttcagcggg caaaatgccc gcttcacacg agtcaggcaa aatgcctgac ccaccccgca
    20941 agccgctgct cctcctcgac gagcccacca ccggcctgca tttcgacgac atcgccctgc
    21001 tcgtgaagct gctccagcgc ctcgtcaacg aaggcagcag cctcctcgtc atcgagcaca
    21061 atctggatgt catccaatgc gccgactgga tcatagacct cggccccgaa gccggcgcgg
    21121 aaggcggcca gttcgtcttc accggcacgc cggaagacct cctcctctgc cgcgaatcct
    21181 ggaccgggaa attccttgcg gagaaattcg aaaatcgaaa ctcgaaatcc gaatccctga
    21241 ccctggccga ggcgcctgcg aagtatgccg cttcgaaact tcgagcttcg aatctcgaat
    21301 attccaactc gatctccatc cgcggcgcgc gcgagcacaa cctgaaaaac atctcgctcg
    21361 aaatcccgcg cgacgagttc gtcgtcgtca gcggcctcag cggctcgggc aaatcctcgc
    21421 tcgcctttga cctcgtcttt gccgaagggc agcgccgttt cctcgacagc atgtccgtct
    21481 atgcgcgcac ctttgtggag cagatggaga agcccgaggt ggacctcatc agcggcgtgc
    21541 cgcccaccgt ggccatcgag cagcgcatct cgcgcggcgg cggcaagagc accgtggcca
    21601 ccgtcacgga agtctggcat ttcctgcgcc tgctgttctc caagctcggc acccagtatt
    21661 gcccgcgctg cgaggtgccc gtgcagaaac agagcctcgg agccatcacc cagaccgtca
    21721 tggcgcggct gaaaaaaggc ccgtgcaagc tgctggcgcc gctcatcaag gcgcgcaaag
    21781 gctttcacac cgaggtggcc gaggccgcgc gcaagcaggg catcaccacc ctgctggtgg
    21841 atggcgaact gcgcgacacg atgaacttca aaaagctcgc ccgcttcaag gagcacaaca
    21901 ttgacgccgt cgtcgccgag ctggacggca gcctcgacga gctggccctg cgcccgcatt
    21961 tggaaaaagc tctcaagatc ggcaaaggca cgctcaagct gcgcctgccg gacaaatcct
    22021 tccaagtcct cagcaccgag atgagctgcc cctcgtgcga gctatccttt gaagagctcg
    22081 acccgcgtct tttctccttc aacagcccgc acggctggtg ccccgcctgc cggggcttcg
    22141 gcttcgaagt cgccgcgcac ggtgccacac cgcatcgcgg cgatgtctcc cagctcgagg
    22201 ccgagctgga agaagagcgc cgcctctccc gcgctgccga tgaggatgag gaaaacggcg
    22261 agcgcgtcac ctgcaaggcc tgcaatggct cgcgtctcaa cgccaccgga cgagccgtgc
    22321 gcctgcaaga ccgccgcatt caggagatca acgccatgtc cgccgccgat gccgccggca
    22381 tcgttgcgcg cttccgtttc gatggacggg aagccgtcat tgcgcgcgac atcctgaagg
    22441 agatcgagca gcgactgcac ttcatgaagg aggtcggcct cggctacctc cagctcgacc
    22501 gcagcgccga caccctctcc ggcggcgagg cccagcgcat ccgccttgca gcccaactcg
    22561 gctccaacct gcgcggcgtg ctctatgtgc tcgacgagcc caccatcggc ctgcatccgc
    22621 gcgacaacga gcgcctgctc gacaccctct ccgccctgcg cgacaaggga aattcattgc
    22681 tcgtcgtcga gcatgacgac gacaccatgc gccgcgccga caccatcatt gacctcggcc
    22741 ccggcgcggg ccgcttcggc ggcgaggtca tcgcgcaggg gccgctcaaa gccctcatca
    22801 aggacaagcg cagcgtcacc ggccaggcct tgcgcgaacc gatgaagcat cccttgcgcg
    22861 gcagcagaag gcccatatgg ggaacaggcg tcccgcctgt ttcatcaaag aagaaaacag
    22921 gcaagatgcc tgttccccat gcggaatggc tcgtcatcga aggcgcgcac gccaacaacc
    22981 tcaagagcat cgaggtgcgc atccccgccg gccggctcac cgtcatcacc ggcgtcagcg
    23041 gcagcggcaa gagcacactt ttgcaccagg tcctgcaccc ggcggtgaaa gccgcgctca
    23101 gcaaaaagaa ggccgccacg gattccgagc cgctctggaa atccgtgcag ggcgccgggc
    23161 agttcgccgc cgtctatgag gtggaccagt cacccatcgg caagaccagc cgctcctgcc
    23221 cggccaccta tgtgggcatc atggatgaca tccgcaaact cttcgcgcag cttcccacag
    23281 cccgcgcgcg cggctatgac gcgggacgtt tctccttcaa cacggacggc ggccgctgcg
    23341 agacctgcgg cggcaacggc gtcatcaaag tggagatgaa cttcctgccc accacgcgtg
    23401 tgcattgcga ggtgtgccac ggcctgcgct acaacagcgc cacgctcgag atcgaataca
    23461 acggcaagca catcggcgac gtgctgcgca tgagcatcac cgaggccgcc gagtttttca
    23521 gcagccagcc caggctcgcc cgtccgctgc agctcctcgc cgacaccggc ctcggctacc
    23581 tccagctcgg ccagcccagc cccaccctca gcggcggcga ggcccagcgc atcaagctcg
    23641 tcagcgagct cagcggcggc gtctcccgca gcgccaccga aaagctgcgc ggcctcacca
    23701 acaccaaaaa gaacctctac ctcatcgagg agcccaccat cggcctgcac atctccgacg
    23761 tcgcccggct gctcgacatc ctgcaccgcc tcgtggacga cggccacacc gtcgtcgtca
    23821 tcgagcatca ccccgacctc ttcgccgaag ccgattacct cattgacatc ggccccgaag
    23881 ccggcgccga aggcggccag gtcgtcgcca gcggcacccc cgagcaagtc gccaaacaca
    23941 aaaccagccg caccgcgcct ttcctctcgc gcctgctgac tcaaggcaga gcatgacttg
    24001 tgatcttgac atcagtcttg ccagacagac gcttacccaa gctctatcaa ctgcatggcc
    24061 gccgttgcag aaagtgaatg tgacgtaaaa acaatttgcc tgctccttga tcatgtcctg
    24121 attttgcgac catgaatttt cttgattccg tccgggactt gcaaggtacc gctctcttct
    24181 atccctgctc agggtatgac tggctcgagc cgattctgac ctttcttcct gttgtgaaaa
    24241 gcttttggtt tgctgaccct cgactgcacc gttcaggatc cattcccatc gagccttttc
    24301 tggaagccgc atctgagagc gccgatgggc cgctggctca gggacggaga tgcaccaccc
    24361 ggagatctcg agatccagga agaaatgctt caaggcccag ctttgtccgc cgcggcacca
    24421 gcctttgcct ggcagaccac accttcaagc gcctctcctg ggaggtgcgg gagcagtcac
    24481 ctgtgactga atcttccaag ccggatcctg acggcaacga aacaccctgg gtgtatgcgc
    24541 cgttgacggt ctccgagcgc tacctcgacg cttcctctgg cgcagagttt gccattcatg
    24601 cccatcgttg cgacgggcag cgctccttgg acttgctgcc cgaacctttg ggaatttttt
    24661 accttcgagg cgacaatggc agcggtaact gtgagggagc cgaaggttcc agcggcagcg
    24721 gctggctgac acgttcttgg tttgaacccg tattgcggaa gcttgtcccg ggaggtctgg
    24781 tcgtcacgga tggctcctgc tcgggtgatg ggaaggacta tcccgggctt tgcgaatttt
    24841 ttttacaacc gaaacgttgg cgaggatgca tggaaaaagg ccaggccttt caccacaccc
    24901 tgcgggcatc atttcgagtg tgtgggttat gcgggtgagg cctacggacc gaccttggtc
    24961 tggaaggtaa cccaaccttc ataaaccagc aaagtgccgg ggttttggtg ctcacaccag
    25021 ttcatcttcg cggcttcgcc gtcactattc gatggcggga aggagtgccc gctctcttca
    25081 tggcagatga aaccctcccg ccgcaaacgc taccgcatcc tgacggctgc cgccgagctg
    25141 gtgagtctgt ggatcggcag gaagattggc gtgcccaaac agtctcaatg ccccaagcga
    25201 aaggcatcga acgcccctgg gagcggcaag actccgccct ctggaatgcc tgctggaaac
    25261 tgggtggtga gttccaggtg gattgggaga actattaccg ccagcaggag aacaagcccg
    25321 gccggattga ttggcttttc gtgcgtttgg cagagcaaca ccagacggtc gcggagtttg
    25381 agacgatcgt gaatggcacg ctggaaccgt caatggtgga aatcccggcc gcgccgggtt
    25441 tcatccgcgt ggaaacgggg aaaccgggac ggactgttgg ccggggcggg tttggctaca
    25501 atttgcgcag gacggcgagc cagtcgtttt cgtcgggggc ggtgagggtg acgggggtgc
    25561 cgccagagag tttgccgtcc ggggttttca aggggccgcc ctggcgcggg ttgaaccagt
    25621 ggagggtgaa ttccccccga tcttttccga gatcgagccc gacccgcccg ccgtcgggga
    25681 gatagacgag gtaaagcgcg ccgggtttgg cgaggcagta tttgctgttg tcgttgctgc
    25741 tgttgccgat gagggcgttg gcgcaggcca tttcgtgaaa ggggatgttg ttttcacgga
    25801 agaagccgag ggcggcgcgg caccagtccc agcttttgtc gcggctgcgg aagtcctcgc
    25861 acttgaggtc gttttcaggg agcttgtagc cgaagtaata ctccactccg gcgcctccgg
    25921 ccatgaggtt gccccagaga gtgtgcttgc ggatgtcgtc gggggtctgg atgaccttgc
    25981 ccccggactt gccgtcgtag ccctggtagc cggggtcggg aggcacgccg gtgtcggctc
    26041 cgccctgctc gtcgctggcg acgacccagg gcctgccggc ggcggcgctt tcccggatcc
    26101 actggagggt cctgcggtgg gtgtcgtccc atgcgttctg gagcgaggcg ccggtgaggc
    26161 tggactggcc gccgagcagg ggacggtaaa ctttttcctg gtcgccgggg taggtgtgga
    26221 tgacgcggtg gtgctggtag gggtccgtcc gggcgatgta ttcggccatg gcgcgctgct
    26281 catcgggcgt ctgggtgttt tcctcgccga ggttccagtt cagcgcgggc aggtgggcga
    26341 agcgggagat gagctcgcgc aggtagagct tgcgctcgag gccggtgccg ccgccgtcga
    26401 gggcggtgga gacggggcgt ttttcgcgtt tggagccgcg gcgctcgtcg tcgttctcgg
    26461 tctcctggag cttgaagtgg gcgtaaaggc cgagcgcggc ggcgtggtcg aggacgatgc
    26521 accactggtc gagcttggag cagtcgtagt gcagcctggc gtcgcgcgcg gtgaagggcc
    26581 agacgttgtc gccgtcgccg ccggcgttgt aggtgaggaa ggagaaggcg ttgcagcctt
    26641 tggcggcgag gtagttcagc gcgccgatga gtcccctgcc cttgccgccc tgccaggagg
    26701 ggtcgccctg gcgccagtcc tggagatggg gcttccaggt tttgagcggg acgttttttt
    26761 gacgagcctc ggtgccgtcg aagtcggcgt atgcgagcaa cgtttcgggg gcgtcgggtc
    26821 cggccttgag gaagtagcgc ctggatccgg caaactggag gtgatggccg ccggtgtatt
    26881 gcagacggcc ctcggcgcgg aagtcgcgcc cggttttgtc ggtgggggcg actacaaatg
    26941 cgccggtcct gccatgccag ggggcgaggg cctcgccgcc gccgtcgagc gcggcgcgcg
    27001 cgccttgagt gaaggaggtg gtgtaggtcc acttgccggg cttgtccggg gaaaggtggg
    27061 cgcgccagac ggtgccggag tcggcgctgg tctcggcggc attgccatcg gcggcgaagt
    27121 ggcccggcac gcggtggctc ggcgcgccgc tttcatgggt gaaggtgact tcgaaggcga
    27181 ggtcggtgaa cgggttgggc gcggtgccgg tctcgcgggc gaaggggccg tcgagttcga
    27241 gggtgacttt gtgccattgc ctcagctcgc cgcgaagggt gatggagccg tcgccgtcgc
    27301 tcccgccgcg cgcggcttgg tcccggtcca gatgctcctg gatccacttt gccagggtgc
    27361 tgctgcccgt gccgtaaccg ccgggcagct cgcgcatgtc gcgcgtctgg cggagcggcg
    27421 gctcgcccca gtgcgcgggg aacttcggcg catcggccgc gccggcgcag actggaggaa
    27481 gcgcggcgca gaggatgatg aggaaaagac gcgggtgcat gagaattgag ggctgggtat
    27541 tgagcttctg gaatgccggc aatgactcaa tgtaagccat gccgtccgct tttgcgctgt
    27601 ttctttgacg cttgaatcgc gccagcgctg cggcaaggcg gactgccggc aggggggcgg
    27661 gtggttgaca ctccggcggg ggtttggtaa gtgccgggca ttccatgaac gaaccaggag
    27721 aattcatgcc gggcgccatg ccgcgcgctt tcatcatcgc cgaggcgggc gtgaatcata
    27781 acggcgacat cgaactcgcc cggcggctcg tgcgcgcggc ggcggaggcc ggggcggacg
    27841 cggtgaagtt ccagaccttc caggcggcgc atctggtgac ggcggacgcg ccgcaggccg
    27901 aataccaggc gcgcaacacg ggcaaaaccg agagccagta tgacatgctc aagcgcctgg
    27961 agctgcctct ggcggcattc gcggaactgg ccgcgctgtg ccgggagctg ggcatcgagt
    28021 tcatgtccac ggccttcgat gaggagagcg cggattatct ggccgggctg ggcatgggtg
    28081 tctttaaaat cccctccggc gagctgacca acctgccttt catccgccgc atcgccacct
    28141 acggcaagcc ggtgatcgtc tccaccggca tgggcacgct ggacgaggcg cgcgcggcgg
    28201 tggagacaat ccgcgcggcg ggcgatgaca agatcaccgt gctgcactgc gtgacgaact
    28261 accccgcgcc gccggattcg gtgaacctgc gcgccatgcg cacgctgagg gacgcgctcg
    28321 gtgtgccggt gggctactcg gaccacacgg agggcatcga ggcgccggtg atcgcggtgg
    28381 cggcgggggc atgtgtgatt gagaagcatt tcactttgga caaggatctg cccggccctg
    28441 accacaaggc gtctctcgac cccgcggagc tggcggaaat ggtgcgcgcc atccggcgcg
    28501 cggaggtgct gctgggacgc gcggagaaac agcccggagc gaccgagctg gaggtggcga
    28561 agatcgtgcg ccgcagcgtg gtggccgcgc gggacatccc ggcgggaatg gagatcaccg
    28621 aagccatgct ggtgctgcgg cgacccggca ccggcatccc gcccgcgcac ctgcatgagg
    28681 tgccgggacg gcgcgcgcgg gtggacatcg ccgcaggggc gctcattcaa tgggaggacc
    28741 tggcatgaga agcgtggccg tggtgagcgc ggggcggtcg gattacagca tctatctgcc
    28801 cctgctgcgc gccctggacg gtcatgccga ggcggagttg cgcgtgctgg ccacgggcat
    28861 gcatctggag gagcgctttg gccgcacggt ggattggatc gagcgcgacg gctttcctgt
    28921 gacggcgcgg attcccgttt tgcaaaatgc ggacacgccg caggccatcg ccgccgccat
    28981 gagcgccgga ctggcgggct ttggcgggca tttcgaacgc gagcggccgg acatcctggt
    29041 ggtgctgggc gaccgctttg aaatgttcgc ggcggcactg gcggcggtgc cattcaatct
    29101 gcccatcctg cacattcacg gcggtgaatt gagcgaggga gcgatggacg accgcttccg
    29161 tcacgcgctg accaagctga gtcatttgca cgccaccgcc gcagaggtgt atcgaaagcg
    29221 cgtcatccaa ctcggcgagg aaccctggcg cgtgcattac accggcgcgc tggctttgga
    29281 caatgtccgc gccctgccgc gcctcgaccg cgcggagctg gaaaaacgct tccgcctcga
    29341 cctgagcgag ccgccgctgc tcgtgacgct gcacccgacg acgaacgagc acgaactcgc
    29401 cggcgcgcag accgaagctt tattggaagc gctgcgccgg ctggatctgc ccgtggtatt
    29461 caccatgccc aacgccgaca cggcgggcag cgtgatccgc gagcgcatac tcagcttttg
    29521 cgcggagtgg aaaaaggcgc gctgggtgga gaacttcggc cccgagggct acttctccat
    29581 gctgcgcgag gcgcgcgcca tggtaggaaa ctcctccagc ggcctgcttg aggcgccctc
    29641 cttcgccctg ccggtggtga atctcggcat ccgccaaaaa ggccgcctgc gagcgggcaa
    29701 tgtgctcgac gcgccgttcg aggcggaggc catcgccgaa gccgtgcgcc aggccctgac
    29761 ttcggaattc aaagcgcgga cgcaaggaca cgagcacttt ttcggtgacg gccaggccgc
    29821 tccgcgcatc gtggacctgc ttctgcgtac gcccctcgac gcgcgcctgc tggacaaaac
    29881 cttccacgat ctcccctcat gaaatgcgtg cttctcggtg ccggtggtca tgggcgtgtg
    29941 ctcatcgagg ccttccagcc aaaaaccttc gatggcatcc tcgacgcgcg tcccggactc
    30001 ggcagcgtgg cgggcgtgcc ggtgctcggt gatgatgaaa tgctgccccg gctccccggg
    30061 ctcggtttta ctcacttcgt catcggactc ggctccacgg cggactgctc ggcacgcgcg
    30121 cggctttatg aatccgccgt ccaagccggg ctgtcagcgg cggaggtcat gcactccacc
    30181 gcgtggattt ccccctgcgc caccctcgaa gcaggctgcc aggtcctggc gcgcgcggtg
    30241 gtgcatgtct cggcaaagct gggggaaaac gtgctggtga acaccggcgc catcatcgag
    30301 cacgactgcc aaatcgccgc gcactgccac atcgccaccg gagccatcct ttgcggcggt
    30361 gtcatcgtcg gagagtcggc gcacatcgga gctggagccg tcatccggca gggcatccgc
    30421 atcggcgcgc gagcgctcgt ggcggcaggc gcggtggtcg tcaaagacgt gccggatggc
    30481 gggcgcgtgg ccggtgtgcc ggcgcgcgcg ctgccttgat ttggcatggg gctgctctgg
    30541 ggcaagcagg gggggggaca ggcgtcccgc ctgtccacac aggcaggatg cctgtgcccc
    30601 atgcgttcac gcggtgcgcg caggtctgcg cagagaggcc agctcatccc gcaaatcaca
    30661 ggctggtggc ggcggatagc cccaagactc cgccatttca ggtgtggcaa agcaagcggt
    30721 caggtcaatc gggacaatct gcccgtccgg ggtctcgatg aagttggagc gcttggtgtc
    30781 accaatgaca agaccatcca cagggcggta ccagatgagg gtgtccacgc ggtcaaagcc
    30841 cagcctgtcc cgaagatgca gcgtgatttc cttctgctcc ggcagcgcgc cttccagctt
    30901 cggctgggag atgacgagcg agggcaaatc cccctcaatg acaccctcaa aacggatggc
    30961 gtcttgaaac acttcgttgg cgaggatcat gcgctccagg tattcgagcg gagcggcgga
    31021 gccaatggcg gagttgtatt cgccatgccg gttctggtgc gtgacgacgg tcaagcccgc
    31081 ctggccgggg tgggtgattt tgaagaaaac atccggatca tgggaactcc ggtaaacgac
    31141 atgctcccct ccacgatcaa actcctcgcc caagagtgaa acgtcaaatc gcgaactttc
    31201 gcggcaccaa ctcaccagtc gtatgaactg ctcaaaactg gaggtccttc gatcttgagc
    31261 cgcaattccc ggttgcgctt catttgctgc tcggttttca ccggatcggt cagtatccac
    31321 ttgcggcggg cgcggcccat cttgtgctgc tcctcttgga tctgttcgag ggttgggcgc
    31381 tgggggatgg attgcatgga gggccggaag gaaggtttgg gattcaggtg tcacctgtga
    31441 ggcaaggcta agggaaaggc gctgcttgtc aatcacggca aaaccggcgg aaagtcccgg
    31501 cccggccgca tcttcgagct tcgaatttca aatttcgaat ttgtcatccc ccccctcccg
    31561 tggcaccgcg tttgcctttc ctccaaacca tgtcatccaa gcccgtgagc caactctgcc
    31621 tgccgcctga cggcacgctt tgggacgccc tccaggtcat tgacaagggc gcttgcggca
    31681 tcgccctgct cgtcggcgcg gaccagcgcc tgctcggcac cgtgacggat ggcgacatcc
    31741 gccgggcatt gctcgcaggc gcgtccttgc aggaccgcgt ggagccgcat gcgcggcgcg
    31801 gcttcaccag cgtgaacgcg gtcacgcctc gcgccgaagt gctggacctc atgcaggcgc
    31861 ggcagattca tcaaatcccc attctggatg acgagggccg gctcaaggga ctgcacctgc
    31921 tgcatgagat catcggctcc gcgccgcgcc cgaactgggc cgtcatcatg gccggaggca
    31981 aaggcacccg tctcggcgag ctgacccgcg ccactcccaa acccatgctc aaggttgccg
    32041 ggcggcccat tttggagcgg ctcgtgctgc atctcgtggg cagcggaatc cggcgtgttt
    32101 ttatttccac caactacctc gccggcatca tcgaggatca tttcggtgac ggcgcgcgct
    32161 ttggctgcgc catcgaatac ctgcgcgagg aacccggcga gccgctcggc accggcggct
    32221 ccctggcgct gctgccggag aggccggagc agcccgtgtt tgtctgcaat ggcgacctcg
    32281 tcacgcaagt ggatgtggcg ggcatgttcg gctttcacga ggagggcggt catgcggcga
    32341 cgatcggcgt gcgcggttac tcgcatgaga ttccctttgg ctgcctggat gtcagcgagg
    32401 ggcgcgtgac cagcatcgtg gagaagcccg ccatctccca gctcatcaac gccggcattt
    32461 acatcctctc gccggaagtc atcggacgcg tgccgcgccg gcacttcccc atcacggaac
    32521 tcttcgagga agccctgcgt cttggcgaca gcgtgggcgc cttcgagatc gccgacgagt
    32581 ggctggacgt gggccagagg gagcaactca aacaagcgcg tggaggcgcg cactgacaca
    32641 tcatgggatc atttgaaaac caaaccgtgc tcgtcaccgg agcgggcggc ttcatcggca
    32701 gccacctggc cgagcacctc gtgcgcgagg gcgcgaaagt gcgcgcgctc gtccattaca
    32761 acgccctcgg caaacgcggc tggctggacc actcggacct ggccggcgac atggaaatcc
    32821 tggccggcga catcaccgac cgcgacagcg tgcgcgccgc ggtgaagggc tgcggcaccg
    32881 tcttccacct cgccgcgctc atcgccatcc cgtattccta tcaagcgccg cgcagctatg
    32941 tgcgcgccaa catcgagggc acgctcaatg tcatgcagtc cgcgctggag accggcgcgc
    33001 gccgcgtgct gaacacctcc accagcgagg tttatggcac cgcgcgcttc gtgcccatca
    33061 ccgaggagca ccccctgcag ggccagtccc cgtattccgc ctcgaagatc ggcgcggaca
    33121 aaatggccga ggccttcgcc gcctcctacg aacttgaggt cgtgacagtg cgtcccttca
    33181 acaccttcgg cccgcgccag agcatgaggg ccgtcattcc caccatcatc acccagtgcc
    33241 tgcgcggcga cgaggtgcgg ctcggcgcgg tgacgcccac gcgcgacctc aactatgtgc
    33301 aaaacaccgt cgagggcttc tgcgcagccg ccctgtcccc gaacgccgcc ggcgaggtca
    33361 tcaacttcgg atcggggcgc gagatcagca tcggcgacct ggccgtgctc attggcaaaa
    33421 tgctcggcaa ggacatccgc gtcgtgtgcg agcaggagcg cctgcgccct tcgaagagcg
    33481 aggtggagcg cctgctggcg tgcaatgcca aagccaggtc cctgcttggc tgggagccgc
    33541 gcgtgagcct ggaggaaggg ctggaaaaga cgctggcctg gctccaggaa aaccgcgcgc
    33601 attatcaggc tcaaggttat gtcgtctgac acgcccttca tccccctgag cgtgccgcat
    33661 ctcggaggca acgagtgggc ctacatcaag gactgcctcg acaccggctg ggtgtcctcc
    33721 gtcggctcgt atgtgacccg ttttgaaacg gctctcgccc aggccgcggg cacgcccttt
    33781 gccgtggcct gcaactgcgg caccgccgcg ctgcacatct ccctgctcgt ggccggcgtg
    33841 cgccctggag acgaggtgct cgtctcctcg atgaccttca tcgccccggt gaatgccatc
    33901 cgttatgcca atgccgtgcc cgtgctggtg gactcggagg ccgcgcactg gcagatggac
    33961 gtggagcagg tgcggcgctt tttggaggac ggatgcgagc ggcgcgaagg cgtgcttttc
    34021 aaccgcgcca ccggccgccg tgtgaccgcc gtcatccccg tccacatcct cggccatccc
    34081 gtggacatgg ccgcgctcat gccgctggcg gaaaggcacg gcctcacggt catcgaggat
    34141 gccaccgaga gcctcggcag cacgctgcac gggcgcgcgg cgggcggcat cggccatctg
    34201 ggctgctaca gcttcaacgg caacaaaatc atcaccaccg gcggcggcgg catgatcgtc
    34261 accgcgcgcg aggactgggc gcggcgcgcg cgccatctca ccacccaggc caaggccgac
    34321 gccgaggagt acatccatga cgaggtcggc tacaactacc gcctcaccaa catccaggcc
    34381 gcgctcggct gcgcgcagat ggagagcctg cccgcttatg tcgctcggcg gcggcaaatc
    34441 caggcgcttt accaaagcgg cctcgccacc gtgcccggac ttcgttttca aggcgaggcg
    34501 cccggcgtcg tcagcaactg ctggctgacc accgtgcgcg tggatgcggc cgcgttcggc
    34561 atggacgcgc gctccctgcg cgcgcggctc aaggcggagc gcatcgaggc gcggcccctc
    34621 tggcagcccg cgcatctttc cccagcgcat caagacgcag tccacctccc ctgccctgtt
    34681 gcggaagact tgtatcaaaa ctgcctttca cttccttctt cctcaagtgc cagcgacgct
    34741 gacatccaac gagtggtggc agccgtcaaa agcgcggcgg ctgcttgacg cggcagtttg
    34801 catttgcgcg atgaaggatt ccggctttgt gaggtgacaa gcccgcgttc aacggcgctt
    34861 tcgactcaac ccaagccatc ccccatcatg tccgacatcg ccctcgccaa aggccagaaa
    34921 tttctcgaag acaacgccgc caaggaaggc gtcgtcacca ccgccagcgg cctgcaatac
    34981 aaagtcatcc gcgccggaga agggcgcagt ccgtccgcca ccgacacggt cgtggtgcat
    35041 taccggggca cgctcattga cggcaaggag ttcgacagct cctacaagcg tggcgagccc
    35101 attgagttcc cgctcaaccg agtcatccgc ggctggaccg agggcgtgca gctcatgaaa
    35161 gagggcgcgg aataccgctt cttcatcccc tcccagctcg gctacggcac gcgcggcgcg
    35221 ggccgcgaca tcgggccgaa tgaagtgctt atttttgatg tcgagctgct cgaagtggtc
    35281 tgactccggc cttgccttcc gggcgcgccg gagcattcat agacccgtca catggccatc
    35341 aaaatagacc ccgccctgca caaggaaaag aagccggact ggattcgcgt gaagctgccg
    35401 cgcgatccgg ttttctggag caccaaggcg ctcatctccg acctcaaact gcacaccgtg
    35461 tgcgaggagg cccagtgccc gaaccgctgg gaatgctgga gccagggcac ggccaccttc
    35521 atgatcgcgg gcgaccgctg cacccgcgcc tgcggttttt gcgcggtgaa gacggcgaag
    35581 cccttcgccc tcgaagcgga cgagccgcag cgcgtggccc aggccacgaa gcgcctcggc
    35641 ctgaaccaca tcgtcatcac cgccgtggct cgcgatgacg tggcggacgg cggcgcggac
    35701 cactttgccc gcaccatcga ggccgtgcgc gccgaggttc ccggcatcac catcgaaatc
    35761 cttgtgccgg atttcaatgg caaggacgac gcccttgagg cggtcatgaa agcgcgcccg
    35821 cacatcttca accacaacct ggaaaccgtg gagcgcctga caccgctggt gcgcagccgc
    35881 gcccaatacc accgcagcct gcatgtgctc aaacgcgcca aagaactggc cgccgaagcg
    35941 gggacaaaat gcgccaccaa gagcggcctc atgctcggcc tgggcgagac ggaggtggag
    36001 ctgttccaag cgatggacga cctgctggag catggcgtca ccgtgctcac cctgggtcaa
    36061 tacctgcggc cttccgaaaa gcacctgcct gtggttgagt acgttcatcc cgacactttc
    36121 gagaactaca aagagatcgc ccgcaagaaa ggcttccgcc atgttgccag cgcgccgctg
    36181 gtgcgcagtt cgtatcacgc ggctgacttc aagccggagc aggatttgtg atcaatatct
    36241 ggaagggtaa agtcattgaa gacggaacgt catccttacc tgaagtgccc tccagcgcca
    36301 tttcaccaag cggaggctac gcccgcgcca aagcctgcaa atcctgcatc cgccgcgcaa
    36361 gggaggagac gcgcgcacgc gcctccggcg gggtcatgag ggcgcggatt tgctccaggg
    36421 ccatgccgct gtgcgcggcc agccaggcca gttgcgcctc gtcatcgccc gccaggtgcg
    36481 gacgcgcgcg caggacgcgg acgcgcacgg catcctgggc ggcggcgagc agcaggtcat
    36541 cccggcgcag ccgatgaaaa aactggccga gggcagcgag atggtcggca aaatggcgtg
    36601 tctggtgcag cgcggcagcg cgcgccgggc cgaagcgcgg caggctcatc cacagccagg
    36661 caagcacgag caggccgagg gcggtgagcg gacgccaggc gcggctccag agcagctcga
    36721 agaagccgct gttcaacgag acgatgaacc acacccggcg cggttcattg cccgccaggg
    36781 ctgccagcca ggcggcgtgc tcgtgctcgt cgaggtaacg gttgcggaac ggcctggcgt
    36841 gggccagcag cgtgacccgg ccctggccgt gccgcaggct gagcgcggcg gcctccttgc
    36901 gcgtcccggc ggcgtgctcg ccctcggcca gacggcgctt gattgtgaaa tgatgcacgg
    36961 ccggcatctc gaattcgaat tcctgccccg cctgacggta ggcgcgagtc ttcagcggca
    37021 tgtcctccat gtctcccagg tcaatgacca ggtttttctt cttctccccg gtttcggcgc
    37081 cgggcgcctc cagttcgtca ttgtccgcgg gcgggttttc ctccccggca gcagcggcgt
    37141 cttttttctc tgcggattca tccccggact tctccggctg ttttgcgtcc ttgcgtagat
    37201 ttttcagatc ctcgacgagg ctgcccacct tcactccacc ggtggctttg actcccaggg
    37261 cgcgcaggag cggatcatcg ctgtgatcgg ccagggcaag tccgaggccg ccgcctgagc
    37321 cgccccagtc gttgtagggc gcgcagccgg cgagcgcgta aacaaggtgc ccgccctcgc
    37381 gcgtccattg caaaagctgg cgcgcgcgcc cctcgggcat gccgccctcg gcggagagaa
    37441 tgagcatggc gccggaggcg tcagggggga gcttgagcgc gggctggcgg gcggcctcgt
    37501 ggccgaggtc ctggagcagg tgttgcgcgg ccaggaaggg atcaagccgc gccttgccct
    37561 tgtggccgag ttcgatctcc acatcctccc atttgccgga gcaggcggtc agcagcaggc
    37621 aaagcagcgc cgcgggcgca aaaggcgtcc gccgcgcggt tgacggagag tttttttcga
    37681 agggccagcc cgcgcacaaa tgctcgaact cctccacgcg cgcctccctg cccgcatagg
    37741 cggcacgcac ccacagcagt gtcagttggc ggaagaaggt ggacacgccc tcctgaccga
    37801 ggcgcgcggc cagcagcagg cagtcgtcct ctgtgtcgct gccgcggatg ggcaggcggt
    37861 gctgggtgac gaggcgggac agtgatcccc ggtaaagcag gctcagagcc tcgcgcgcgc
    37921 gtccctcccg ccaggcggcg cgggcggctg cgggaatgtc cggcggcagg ctttcagggg
    37981 tgatctccat gcccatgagc acgcgcggcg cggcgggcgt gggaacgggc gggcgggcgc
    38041 cgcgacgaat actgaagaag cccgcattgc gcgccaccag ccagaccagc agcgccacca
    38101 gcgcgccgac cagcagatat cccagcgggc cgaggatttg gctgagaaaa ctcaaatccc
    38161 actcccccga agacccgcca agctccggca cccaggtctt ctgcttgaag acatgctcct
    38221 caaactcggg ccgtgccagc acctcggcgg ctttttgttt caccgcctcc ttgtccaccg
    38281 gctccgcgcc ggacggcgtg gcggcttggg cggtggcaac caggcaggaa aacagcaaaa
    38341 gcaacaccgt caccaccggg cgcaggcgcg cggccaggcg gcggaaggcc agctcgatgt
    38401 cccacccctc cagcttgatg cgcgagttca gatagagacc gaagcccgcg cccgcataaa
    38461 agggctcgat gagcgtgatg gtgacgaggt aaagcagatt gccgagccac tgctgggcgt
    38521 gggtgcccgc cagcgcctcc tccgggttga gcagcatctc ctgaaaatcc ggcaggccct
    38581 cggccgggcc gagcgtgctg gtcagcgcca tgagtccaaa agccgcggcg atctccaaat
    38641 gcacgaacac ccacgtcagc atggcgccgc cgctgccgcc atcactggca agcgtggaca
    38701 cgcgcgcgcg cgccgccctg ccgcgctggc cctccagcat cagcaccggc agggtgaagc
    38761 tgcgcatcag ggagagcctc ctccagagca gcgcgctgaa caaaaagcgc gtccacagtc
    38821 cgggccaggc gcgcagggtc tcccccagcg tcggtttgcc gccgaaggcc gcgcgcgcca
    38881 ggtggtgcag cggcacgcgg tcatagagcg gcttcaacca ccagaccacg agggaaaagg
    38941 ccaccggcca ccgccacagc agcgcggcca gcagcgccca caccggcagc accgtcaccg
    39001 cccacagcgc caggatgcgc ccgtagtccc gccgcaccag cgcgcagccg aggtccgccg
    39061 cctcccacga gctgcgcgga cgcaggacca gggtcaggtc ctcaagccgc atgacgctcc
    39121 tttccgctga aaagcagcca gcccgccgtc agcagccaca tcaccgcgcc gaagctgtac
    39181 ttcaccggag gcggca
//
LOCUS       DAOMYJ010000021        39026 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Prosthecobacter sp. isolate AMR_MDS_5629
            NODE_391_length_39026_cov_22.313002, whole genome shotgun sequence.
ACCESSION   DAOMYJ010000021 DAOMYJ010000000
VERSION     DAOMYJ010000021.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867850
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prosthecobacter sp. (wastewater metagenome)
  ORGANISM  Prosthecobacter sp.
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Verrucomicrobiaceae; Prosthecobacter.
REFERENCE   1  (bases 1 to 39026)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 39026)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.30
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:34:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,742
            CDSs (total)                      :: 4,684
            Genes (coding)                    :: 4,676
            CDSs (with protein)               :: 4,676
            Genes (RNA)                       :: 58
            tRNAs                             :: 55
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..39026
                     /organism="Prosthecobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_391_length_39026_cov_22.313002"
                     /isolate="AMR_MDS_5629"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1965333"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..567
                     /locus_tag="PLP58_03810"
     CDS             <1..567
                     /locus_tag="PLP58_03810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007416781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1501 domain-containing protein"
                     /protein_id="HRJ07682.1"
                     /translation="DVEQEPANIREMYGDTLQGRQILMARRLAERGVRYVQVYHGAGQ
                     PWDSHASLEENHRRLARECDQAIGALLQDLKERGMLQDTLVIWGGEMGRTPTAQLPLT
                     PKIGRDHHTDGFTVWMAGGGVKGGYTHGVTDDIGLSIAEDKVHVHDLHATILHLLGFD
                     HEALTYRYAGRDFRLTDVFGNVVREILA"
     gene            653..2659
                     /locus_tag="PLP58_03815"
     CDS             653..2659
                     /locus_tag="PLP58_03815"
                     /inference="COORDINATES: protein motif:HMM:NF020123.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin domain-containing protein"
                     /protein_id="HRJ07683.1"
                     /translation="MKHAALFSAILFSAAPLVAQTTLMEQRFDAVDKNKDGIVTEAEL
                     PVKMWFRLLDANNDGQITRAEAIAGASRLRGARGMGAAAEEPAEPEDASLKEAPRVLK
                     AAEHGVGHLVPDLSLRDLAGQEKKLSAHLADARGLALIFFGATCPISQKYGPEMSRLE
                     KDAAAHGLRVILVCPVGVETTDYIRQFISGHGLKSPVVHDTTGALAATLAATTTTECF
                     LLDARRTLVYRGAINDQYGLGYAKEQPTRAYLRDAAAALLRGEPPEIAATSAPGCALD
                     VKTAAPAATGVTYHNQISRIMQAHCVECHRKDGVAPFSLETYDDLVENAGMIRKQVER
                     GVMPPWFAAPPAEGHASPWANDTSLSARDRADLLAWLASDRPPGDPADAPLPRNFPAE
                     WLLGKPEVVVQLPQPVAIKAEGTMPYQFVTVPTAFAEDRWVSAYDILPTDRSVVHHVI
                     VQVHEKGARVRNRGEGSEGYWAAYVPGNTHHRYPAGFAKKLPAGATVSFQIHYTPNGK
                     KTQDQLRLGLHFAKEPPRYVVHTAAVAHPRLSIPPHTARHVEVKEQTVPANMNLTALM
                     AHMHVRGAAFKFEATRPDGTHEVLLDIPRYDFNWQLRYDYAQPHFLPAGSRVKLTAVF
                     DNSESNPANPDPSKTVRWGPQTHDEMMIGYFEYFTPRKDDVAME"
     gene            2726..4189
                     /gene="gatB"
                     /locus_tag="PLP58_03820"
     CDS             2726..4189
                     /gene="gatB"
                     /locus_tag="PLP58_03820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009957903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HRJ07684.1"
                     /translation="MPKYIVTIGLEVHAQLTTRSKMFCGCAIAYGEEPNTLTCPTCLG
                     LPGALPVLNQNAIEKTILAGLMLGCETPPVVKWDRKNYFYPDMPKNYQITQFDLPLCL
                     GGGVPLYDLAYPKDAQKSIANPGKVVQLTRIHLEEDVAKSTHHAGFTTIDFNRAGTPL
                     MEIVSEPDIASAEEAFAYLTSLRQILVYGGVSDADMEKGQMRCDVNISVRPEGQEQLG
                     AKIELKNLNSMSAVRRAIKYEAARQMDCLDRGEKLIQSTRRWDDDRGETTLMRTKEDA
                     HDYRYFPDPDLLPLRTPDILARVRPLVPELPHEKRARFEKDYGCSAYDAGVLVSEKAL
                     AAWYEAAIAAQPGVPAKKIANWVINDLLGVLKDSEGGLAACPVRPAQLAALVAIVEAG
                     KISNTQAREVFAEMAASGADPAKVIQVKGFEQVSDTGALEAIVDQILAANPEKVAEVK
                     GGNDKAMNWFTGQAMKASQGKANPKLVTEIVRSKVLS"
     gene            complement(4253..5191)
                     /locus_tag="PLP58_03825"
     CDS             complement(4253..5191)
                     /locus_tag="PLP58_03825"
                     /inference="COORDINATES: protein motif:HMM:NF015431.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HRJ07685.1"
                     /translation="MFEKLFSKGGLTLERLRGFMSMAEAGSIARAAPDDANRQSQISR
                     QIRELEEFFDVELTRRKGRTLSLSPAGARLASLIREQLQDLEDFRRDQRGLPKSFTIG
                     AGASVLEWLVVPALPRLAELLGGATLLIQPMRSRSLAEAVSEGRADFAILRRDAIPAA
                     KSVLPLVKLGFVICIPERLIPQGVSDRQASHPSFWRDLPFAAGRDGGQLDLAIRAAMT
                     DAGLSYRPRFECGSILQARQLVDMGECAAVLPALGLRGMDRKRIRVLPFAPMKGYGRG
                     LVLHWNERQTRRRAVDASTIQAMARSLALTVKEPGP"
     gene            5262..6215
                     /locus_tag="PLP58_03830"
     CDS             5262..6215
                     /locus_tag="PLP58_03830"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HRJ07686.1"
                     /translation="MKQQSYDLIGDIHGQHEKLARLLENLGYRPRPGGGMRHPDGRRV
                     VFLGDYIDRGPDIRRVLETVRGMVESGDALAIMGNHEYNAVLYATPDGNGGHLKEHSA
                     RNTKTHQATLDQFAGRPAEWAEWLLWMRGLPMFLDLGALRAVHACWDARRISRLGGAS
                     LLDPDFLRASATKLTPEHRAVENVLKGPEMLMPEGSCFHDKEGAARRTVRVRWWNIPP
                     QSHVCALAMPEPFDSPGDAAPHDLRRLPCYGSNEPPVFFGHYWLPPEKEKAPLARNLA
                     CLDFSGARQGPLVAYRWDGERELSPEKFLCASLLETTTHTP"
     gene            6212..6628
                     /locus_tag="PLP58_03835"
     CDS             6212..6628
                     /locus_tag="PLP58_03835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ07687.1"
                     /translation="MKTHTSFEAFLTAARENALRMLLNAEYIRRELPSLQVPEGLRAD
                     ILELCDDWCEAKHDAFSLIFDISDIHAEGADIRQHCARLLSWLTQASMKAHAVIIQAQ
                     DSAASSLVTLLVTESAVNVLNANSAAHEAWADHLNF"
     gene            6655..7407
                     /locus_tag="PLP58_03840"
     CDS             6655..7407
                     /locus_tag="PLP58_03840"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF012521.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HRJ07688.1"
                     /translation="MNRTRRARQIQTIQLLKTELEKPGLSAERRQEVSEFLMDCAHDC
                     FMDDMFEPDFLNGIILIRHGESMANAGERTRTPSGIPLSPLGREQARDLEHAALDPEL
                     IVVSAYLRTQETAAPLCQRLSDVPVETWPVHEFTYLAPEHYLNTTEQDRHAPVARYWE
                     RADPQHRDGPGAETFAEFIARVDAILERLRSMDDPQVCIFTHSFFILALLWRQMRPGA
                     VVDERFMREYDIFRRAARIEHARPIPFRIIKS"
     gene            7459..12345
                     /locus_tag="PLP58_03845"
     CDS             7459..12345
                     /locus_tag="PLP58_03845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009965037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008705 - methionine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12 dependent-methionine synthase
                     activation domain-containing protein"
                     /protein_id="HRJ07689.1"
                     /translation="MPARPNCRPELEAALRQRILVIDGAMGTTIRQYKAKGVLDEASA
                     RGERFRENEKDILNNGDILSVTRPDIIEDIHKRFLEAGADIIETNTFSATSIAQAEFF
                     REGKKNPEFFQGIIEDTFLNDLAWEINFESAKQCRKWADIVAEQTGRKRYVAGAIGPL
                     TVSLSQFPDLSDLSFRYVTFDQVKQAYKHQVRALIAGGVDTLLVETIFDSLNAKTALV
                     AIREVFEEDKLELPVQISAAVGPGGETMISGQVTEAYLNAMRHVKPLSIGLNCSLGPD
                     KMRPFLAELAAKADCFVSCYPNAGMPNPLAPTGFDLLPPDMAGYATDFASSGFVNIMG
                     GCCGNTPEHIAAIAKAVERFAPREVPDDSHDMRLSGSQPFVLKSWGTARGTGVSPVQT
                     EGRQDARPPYLMIGERTNVAGSPKFAKLIKENKLEEAVAIARQQVESGANVIDVCMDE
                     GLIDGVPMMTKFLTLLQTEPEVNKVPIMVDSSKWEIIEAGLKCLQGKGIVNSISLKEG
                     EAKFKEYANKIKQYGAATVVMAFDEQGQAATYEDKIRICERAYRILVDEVGFPPEDII
                     FDPNILTVATGIEEHNNYAVDFIEATRWIKANLPHAKVSGGVSNISFSFRGNNKVREA
                     MHSVFLYHAIKAGMDMGIVNAGMLEVYEEIPPEMLVKVEDVILNRNKEATEVLVDYAE
                     QFKGQAGSAKKAEIDLSWREASVEKRLEHALLKGITDFINEDTAEALAKLGKPLSVIE
                     GPLMDGMSVVGDLFGAGKMFLPQVVKSARVMKQSVAYLQPYMEAEKARKARERELLIE
                     IAEKTVKALESGSDVTAVEGFTYTPYPGLSLEERRIEVKFAAELAADLEAAATEYKRR
                     FANVLDRNNVQELSPDYAATRESRQKWSIATLEPAGAFVDWFFAKVLSTAQSKDTIIF
                     NAGGQGSGKTTATADIKVTGNAILVIDGTLQNHARSVEHFRPAFAAGCSIELRFVYCN
                     WPDAVRNMAGRAAKGAGRVVPLKRAAKGHFEAARTTLKLCGEFADKPELDVFVVENRV
                     GALPRSLNLAWLSEHLHNSVEELLQTGQTTLQDEFKLHSDDPDYSARLLAQFQATSEP
                     GSGGAVSSDRGRHVGGSEPGLGSGQPGDGKASGSTAASSLTAADLIPAEAAKQGGGKI
                     VLATVKGDVHDIGKNIVGIVLACNGFEVTDMGVMVPCQKILDKAVEVGADVIGLSGLI
                     TPSLDEMVHVAAEMERLGFKQPLLIGGATTSAAHTAIKIAPKYSGSIVHVLDASRSVP
                     VTTALLSEDQREGFIKSNEERHAKLREEYGRKKDRELLSLAESREKGQKFDWSTQDIA
                     TPSFTGTKVYEGADLISTLREFIDWSPFFHSWELRGRWIANEGRFSSAHEDAEMKLKA
                     EAEALKLYNDANTLLDRIIAEQRFTAKGVFGLFPANSVGDDIEVYEGGKLKTTFHTLR
                     QQVIKKDKANFALSDYVAPKSSGRTDHIGGFCVGIHGADEFAKEFDAAHDPYHGILTK
                     AVADRLAEAFAEYLHQQARFAWGYEKPGDLSMEDTVKEKYRGIRPAPGYPAQPDHTEK
                     PILFALLDATAKTGVELTESMAMHPGSAVSGLYFAHPESHYFGISVLGKDQVEDYAKR
                     KGMSVEEMERWLGPWLGY"
     gene            complement(12363..14456)
                     /locus_tag="PLP58_03850"
     CDS             complement(12363..14456)
                     /locus_tag="PLP58_03850"
                     /inference="COORDINATES: protein motif:HMM:NF024126.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetylxylan esterase"
                     /protein_id="HRJ07690.1"
                     /translation="MKFSALLLLLAALPVSARFPEATPDSPGRRMLDRYFQQQVREIE
                     ETGGLKHIGSAEQWREHEPEYRRQLAEMLGLEPMPERTPLLPVKTGELKGEGFRVEKM
                     HFQAVPGYYVTANLYLPEKVEAPCPAILYVCGHANVVKDGVSLGNKTGYHHHGVWFAR
                     HGYVCLIIDTVQLGEIRGEHHGTYSKGRWWWFSRGYTPAGLEAWAGMRALDFLETRLE
                     VDKTRFGVTGRSGGGAYSWWVAALDERIKAAAPTAGVTSLKNHVVDGCVEGHCDCMYF
                     VNTYRWDFDRLAALVAPRPLLIVNTDKDSIFPIDGVFQVYQNTRRLYSLLGQEKNIGL
                     QVAEGPHSDLQPLNMGAFHWFERHLKGADAMQVLHQGAEKCLDPAALRVLAETPADER
                     NTTIDETFVPQAAAPAPPTNAGEWEAQSAKWMQGLREKVFAGWPKDAPGIQPVKEGGM
                     ERDGIQMSVFDLVTQQPFRLRLHITHRAGLRLEDLELVALNVLDEQGWQDFCNTYESR
                     FAKLFDSFPQGEADETAFTSERKMFENFKWGMAYLCPRGIGPTEWTGSEKAQTQRLRR
                     FYLAGQTLDSMRVWDIRRAIQAVREISGLKETPLWLQAHRDMAANTLYAALFEEGITR
                     LDLHDLPTTHMQGPAYLNVLKILDMPQAAALAASKTRVVLHTADETPWEFPSTVAGNL
                     AWPEKQWQIRKPPGE"
     gene            complement(14733..16451)
                     /locus_tag="PLP58_03855"
     CDS             complement(14733..16451)
                     /locus_tag="PLP58_03855"
                     /inference="COORDINATES: protein motif:HMM:NF012759.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantetheine-binding protein"
                     /protein_id="HRJ07691.1"
                     /translation="MPAPDFSDLLTRARRIICADLLATGPDFQDSSDLFDAGLDSMAI
                     MQLLLLLEEEFGSPIPVETVTRENFRTVHGVALLVASRLGIEPPAVAQPANPAAPEPP
                     AGEKPAPVQDAPAQTFTTLPLKNCDYFVLSFDAMMRAKGEGGHVAHSLMELDAVPDVP
                     ALRRALELAADRYPLLSARLQKTGLFSPHHWVAGLERLPPDLLLISENGSPGALPGAQ
                     PCADADAEIASLINTPIPCRDDARWVKMRLALVERRNGTATLMLSWSHFVVDGKGAEL
                     FLQALHDLGSAGATAITALDTAAPQPDPRHYGQKWQATFPITGRFEEITKHRFDSLAP
                     RKGGQSGPIRYEVRLLDDAQTAAVKARSRSFSEVLNLPFQLACAMRAHRAVFQHRGGV
                     PESLLCSVPIQTRRPGAAGPIFQNHLRMFFGQCLREELRTLEGAVSSIAAQHQRYLKD
                     GLDKAFDELMNVMRPVPPGIYMKFVSWRMKGLFNSFFHSDTGEFAPGLTAFLGAPVRT
                     ACHIPGFTSPPGTGLFVNEKHGRLVLAACWRGNVLSERERAILWERWLADLGATPAFT
                     GGWLAE"
     gene            complement(16461..17321)
                     /locus_tag="PLP58_03860"
     CDS             complement(16461..17321)
                     /locus_tag="PLP58_03860"
                     /inference="COORDINATES: protein motif:HMM:NF013862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HRJ07692.1"
                     /translation="MQNLPDARPDVAVSLSSAFLGFHSHAGFLQGLVEGGVWPGQIAG
                     ASSGALVAALAAAGMSPLEIFELLQTRRFRWSFFEWGMLYGGAFLPVHKRKHTGGIQG
                     RAILRYLSGIFGGRRIEDCAPRLGLAVTNLSSRRAEIRTRGPLAETVVASCAFPGVIS
                     HQIIGGEALWDGGIAQNPPFMHWLGEAAVSRVLVHDVGVKDTPSADRIGLIPAFALAH
                     DVVMAELHELRLEKFAEAGKTLERFRTASPRPGILVREKTGRACFELGRLSGRRAAET
                     LSPSPALPAL"
     gene            complement(17378..18730)
                     /gene="thrC"
                     /locus_tag="PLP58_03865"
     CDS             complement(17378..18730)
                     /gene="thrC"
                     /locus_tag="PLP58_03865"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009960788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004795 - threonine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HRJ07693.1"
                     /translation="MFYISTRGQTRPHTFTEAVEAGLAPDGGLFLPEHLPDISGRLAG
                     WAELAYAELAAEFFQIFAPDIPAEEWRALTRQAYARFDSPEVAPLRKLDDKTYVLELW
                     HGPTLAFKDFALQLLGLLYKRQVRLTGKKLAVLGATSGDTGSAAIHGCMGQDGIRIFI
                     LYPHGRVAPLQERQMACTGADNVFAIPVPGTFDDAQRVVKDCFGDSDFTARVNLSAVN
                     SINIARVLAQCVYYIHAWLRLPEEARTAAEFVVPTGNFGNVLAGWLAHRMGMPCGGFR
                     VATNQNDILCRFFESGDYRQGDVHPSHAPSMDIQAASNFERYLYYLVQGDQLRVRELM
                     QRMKAGQHVTLPKVDLGFRASRMDDSAIEETIARVWKEHAYVVDPHTACAFTGMAPDR
                     VSVVLSTASPAKFPEVVARATGAEPLHPSLEALKDKPLQTHPLAAEAEAVKSFIEGNL
                     "
     gene            complement(18852..19934)
                     /locus_tag="PLP58_03870"
     CDS             complement(18852..19934)
                     /locus_tag="PLP58_03870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ07694.1"
                     /translation="MNEDTPRSSLWSRFVKKWRAVGGGSLTLSVAIHLGILLFAGAIV
                     VSTQMIQEQVDFLPGGGSQQGAAASAEMRHKVQQKKRSQLNKVMPMKKIVTTNMNSEI
                     SLPEAPLDLLDVPDMSSLLGGGSLGSGGFGKAGAGGGFGTGLGRGGMGGVTFKPIMMF
                     GMELKDARKIAVVMDVSRSMTRYLPIVAKELDKVAFGSPLVLYFGCGLQKPPRDMDDK
                     VRKAQGDEFARFWQHWQGKASLRMTAEERKKLVYDPNTPMPLEAIYAQMVKRPNTYFI
                     DFNGITYTSPALMCKEVMEADTIYWFADFQDRVDEAHMEEVFKKLKSRKQKLYIHASI
                     RGRSFEQVRDKLVLPLGGEVIETKAE"
     gene            complement(20040..20420)
                     /locus_tag="PLP58_03875"
     CDS             complement(20040..20420)
                     /locus_tag="PLP58_03875"
                     /inference="COORDINATES: protein motif:HMM:NF014448.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor biosynthesis protein MoaE"
                     /protein_id="HRJ07695.1"
                     /translation="MHYLLSHDPISDTTPPFGPGLGAEARFLGVVRDLEDGRVIAGIE
                     YSAYVPMAEKMLAAIIAEARAAHGGHEVFIQHRLGFVAAAEVSILIRVRARHSAPAFD
                     LCRSYLHAVKTRVPIWKRPAETAA"
     gene            20569..21690
                     /locus_tag="PLP58_03880"
     CDS             20569..21690
                     /locus_tag="PLP58_03880"
                     /inference="COORDINATES: protein motif:HMM:NF012692.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HRJ07696.1"
                     /translation="MRKNPVDQSRLAAQVILNVRSGRATSRRGLAEALSVSPSTTGLY
                     VDELVKAGLLRETGLEQGKMGRPKRRLSTVPGAGWFAGLEFHAGRVQAARVDFSGRLI
                     HSQSFALPANPTTRTVMEQLREAVAWLAGRSPGRMLAIGVGAPGVVDPKNGTGLHYSF
                     IEDWRDVPVAEELGFEFGVRVTLENNLRVIALAERWFGDGGGLANYIVLGPRRGFGVA
                     IVNEGQLLGGAHQAAGEVGNWWWPAGGAMREMHDELSAPAVWRRLAGAAETEKLPADL
                     HQALALVARNSAREEVVRDFAKVTGWLQLLLDTEAFILHGPLTALGDSFWNEVSEETA
                     RLMPRLAARQPRITCSSLTDNAGALGAASLAMEAWQPSS"
     gene            complement(21687..22430)
                     /locus_tag="PLP58_03885"
     CDS             complement(21687..22430)
                     /locus_tag="PLP58_03885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009966071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HRJ07697.1"
                     /translation="MASMNIYETRRLLDEYLLFHYGAAKEVLPWERGPHEALNFARRT
                     VHELLDKDALPPGARALDLGCAVGRSSFELTRFAAEVIGLDYSLSFVGAARTLAHEGC
                     LDYERLDEGRASTLLRASLPEGARVEAAAFEQGDAMSLRAGLGGFDIVHAANLLCRLS
                     DPARLLERLPDLVRPGGQLLLATPCTWLEEFTPAANWPAGSTRDWLKDRLSACFTLEL
                     EKDMPFLIREHARKYQWSVALGMRWRRLT"
     gene            23441..23515
                     /locus_tag="PLP58_03890"
     tRNA            23441..23515
                     /locus_tag="PLP58_03890"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:23473..23475,aa:Trp,seq:cca)
     gene            23738..23815
                     /locus_tag="PLP58_03895"
     tRNA            23738..23815
                     /locus_tag="PLP58_03895"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:23773..23775,aa:Asp,seq:gtc)
     gene            23825..23900
                     /locus_tag="PLP58_03900"
     tRNA            23825..23900
                     /locus_tag="PLP58_03900"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:23859..23861,aa:Ala,seq:tgc)
     gene            23910..23983
                     /locus_tag="PLP58_03905"
     tRNA            23910..23983
                     /locus_tag="PLP58_03905"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:23942..23944,aa:Asn,seq:gtt)
     gene            23998..24077
                     /locus_tag="PLP58_03910"
     tRNA            23998..24077
                     /locus_tag="PLP58_03910"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:24034..24036,aa:Arg,seq:tct)
     gene            24356..24430
                     /locus_tag="PLP58_03915"
     tRNA            24356..24430
                     /locus_tag="PLP58_03915"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:24389..24391,aa:Gly,seq:tcc)
     gene            24522..24594
                     /locus_tag="PLP58_03920"
     tRNA            24522..24594
                     /locus_tag="PLP58_03920"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:24554..24556,aa:Gly,seq:gcc)
     gene            24602..24677
                     /locus_tag="PLP58_03925"
     tRNA            24602..24677
                     /locus_tag="PLP58_03925"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:24635..24637,aa:Thr,seq:tgt)
     gene            24938..25012
                     /locus_tag="PLP58_03930"
     tRNA            24938..25012
                     /locus_tag="PLP58_03930"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:24970..24972,aa:Ile,seq:cat)
     gene            25125..25199
                     /locus_tag="PLP58_03935"
     tRNA            25125..25199
                     /locus_tag="PLP58_03935"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:25157..25159,aa:Gln,seq:ttg)
     gene            25208..25283
                     /locus_tag="PLP58_03940"
     tRNA            25208..25283
                     /locus_tag="PLP58_03940"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:25241..25243,aa:Leu,seq:cag)
     gene            25373..25448
                     /locus_tag="PLP58_03945"
     tRNA            25373..25448
                     /locus_tag="PLP58_03945"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:25406..25408,aa:Leu,seq:caa)
     gene            25518..25593
                     /locus_tag="PLP58_03950"
     tRNA            25518..25593
                     /locus_tag="PLP58_03950"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:25551..25553,aa:Arg,seq:tcg)
     gene            25815..26840
                     /locus_tag="PLP58_03955"
     CDS             25815..26840
                     /locus_tag="PLP58_03955"
                     /EC_number="6.5.1.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR02306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA ligase (ATP)"
                     /protein_id="HRJ07698.1"
                     /translation="MKRKLASIQSVLAIEPIPNADAIELVRIQGWQCVVKKGEFRVGD
                     VGVFFEIDSVPPDDERFAFLWRPKDGSVVPQPATFRLRTIRLRGMLSQGLLMPLRQFP
                     EIPSGLTTSADVTEILQITKWETQIPLNDEVDGPFLPGVPKTDEMRVQSVPDVIAEIT
                     GKPYVITVKCDGTSATYGMNRHTGVFTVCGRNWSIKRGTNAYWHAAEKYDLEALLGRF
                     PQWIIQAELVGPGIQKNRLGLKGIELRVFNVYDQHVGRYLDHDEASAILSDRGLSMVD
                     ELERGESFSHDQASLLALAEGFYPGTQNEREGLVIRPQTETHSQVLGGRLSFKAISNR
                     FLLKGGD"
     gene            27869..27945
                     /locus_tag="PLP58_03960"
     tRNA            27869..27945
                     /locus_tag="PLP58_03960"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:27903..27905,aa:Glu,seq:ttc)
     gene            27954..28029
                     /locus_tag="PLP58_03965"
     tRNA            27954..28029
                     /locus_tag="PLP58_03965"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:27987..27989,aa:Leu,seq:gag)
     gene            28356..28808
                     /locus_tag="PLP58_03970"
     CDS             28356..28808
                     /locus_tag="PLP58_03970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ07699.1"
                     /translation="MKLTKALKLKNQLAGDVTTLKDRLTKQNSRAASVPFDYDSRQVL
                     ADLRTKLDDLVKVKASIAVANAAQYERIFRLAEIKGLVALLKSLDVRQGVFKEGGSYM
                     QAAFEVEYIAQIKKAEVDQLVAELEAEIITLQDALDEFNHTHSVAQAA"
     gene            29083..29862
                     /locus_tag="PLP58_03975"
     CDS             29083..29862
                     /locus_tag="PLP58_03975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009958464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HRJ07700.1"
                     /translation="MQNNEVFQHVRDALARLESERNVRVLYACESGSRAWGFASHDSD
                     YDVRFLYVHSSDWYLSVEDRRDVIEEPLNDELDVSGWELRKALRLLRKSNPPLLEWLK
                     SPVVYQHDAVFTREFGELSERFYSPRRCFAHYLHMAVGNWRSYLEGREQVSLKKYLYV
                     FRPMLACRWIERSLGQVPMLFNELVEGVLEEREVRDALNALVARKQAGDELSVAPPDE
                     VLSRFVVAELQRLEALNEPEEVAGDVSVLNSFFRRYALNGV"
     gene            complement(29939..30229)
                     /locus_tag="PLP58_03980"
     CDS             complement(29939..30229)
                     /locus_tag="PLP58_03980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ07701.1"
                     /translation="MPHASSAMQTVIIGREPVAMLLEGSFNALLGDAARSPCFVHLRV
                     EFIDKLTQLQLQELLPGLLQAMIGKLVQLLMDQSSERLGISESGRHGVISIH"
     gene            complement(30213..30641)
                     /locus_tag="PLP58_03985"
     CDS             complement(30213..30641)
                     /locus_tag="PLP58_03985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ07702.1"
                     /translation="MFHFLIEEAALVVCGAAHADQRMRQMAGRAFDHGKMKSLCREFV
                     KQNAQQVHSLLQTFWPQFNIPGSQHIRLVAQTFIDLQEERHEADYDMSVVFSRQEALN
                     AVSRVDAAIHSWRHLKQASRDTCRLFAISLALWPSLASRQ"
     gene            complement(30755..31069)
                     /locus_tag="PLP58_03990"
     CDS             complement(30755..31069)
                     /locus_tag="PLP58_03990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ07703.1"
                     /translation="MPLSRTSPVNLKDILARIQPQLIAKKGFSRPQDVQLLKGKDSDG
                     EDALFVYLVFPDKTPDSELAWSKVEPMVSWVRDTVWSSADEEQWPYVRVKRASEITKQ
                     IG"
     gene            31217..32632
                     /locus_tag="PLP58_03995"
     CDS             31217..32632
                     /locus_tag="PLP58_03995"
                     /inference="COORDINATES: protein motif:HMM:NF013319.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008452 - RNA ligase activity [Evidence
                     IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RtcB family protein"
                     /protein_id="HRJ07704.1"
                     /translation="MKPNDLIRLGVPQGAAISSGMDFIAKFMAQGGDSARLEEELGAI
                     IAKPESFLGDPLRESFARDLYAPAYKQRDVLAPWAQWGSGLEAQAVQQMANACALPVA
                     VAGALMPDAHVGYGLPIGGVLATEGCVIPYAVGVDIACRMRLTVYDRKASTIAGQKDR
                     LANILEDETRFGIGSTFKDRRDHDVMDEDWDVSPITRRHKDKAWSQLGTSGSGNHFVE
                     FGAFNVSADESVALKESGFDIKPGEYIALLSHSGSRGAGAQVCQHYSRIAMDRRYDLP
                     KEMKHLAWLTFEEEAGQEYWAAMNLMGRYAAANHALIHKHIAKKVGAHVILDIENHHN
                     FAWKETHVVNGAEREVIVHRKGATPAGAGVLGVIPGSMATPGYVVRGKGKPESLLSAS
                     HGAGRVMSRSKAKESFTWNAVKKQLAAAGVELLSAGIDEVPGVYKDIHGVMAAQTDLV
                     DVLGEFRPRIVKMCPEGPAED"
     gene            32864..33466
                     /locus_tag="PLP58_04000"
     CDS             32864..33466
                     /locus_tag="PLP58_04000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007413951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HRJ07705.1"
                     /translation="MSDVLNKTTVLVLNRNWQAVGVKTPAEAFGMMAAGNATALQISG
                     DDFMSPVRWEDWLRLPVRDDDNSIGTVHGPVRVPTVLVLARFDKVPKRRPKFCAKAIW
                     ERDGGVCQYTGRKLKPNEGNIDHVVPVSRGGKSTWDNCVLADKKVNSKKGSKLPDEAG
                     LKLLRRPFAPREVPVTHLIRNTYKVRDWEMFLAHPGVHDS"
     gene            33694..35472
                     /locus_tag="PLP58_04005"
     CDS             33694..35472
                     /locus_tag="PLP58_04005"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007418859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase"
                     /protein_id="HRJ07706.1"
                     /translation="MSDAKSSLAQMEKIVSLCKRRGFIYQSSEIYGGCGGVWDYGPLG
                     AELKRNLRTAWWNTMTREREDVLGLDASILMNPAIWKASGHVDTFADLMRECSLTNKR
                     VRADHVDPQEGVVMNYSGAKAPNGWKLARAISVLMKKGEHIESLRKRVRTLIASNAGE
                     AAGKVEEIELLDEAKGDTLEKTIDFHPETGGALGEARPFNLMLKTYLGPTATEADVTY
                     LRPETAQAIFAQFKNVFDSSRVKVPFGIGQIGKAFRNEVTPKNFTFRSREFEQMELEF
                     FIKPDEAVEIISGEVAKWHEGADLSEPQPNWGWEMWHRYWVDQRTKFYEGIGLGDVLE
                     YYWQTPADLAHYARACVDILCEFPFGTEELEGIAARGDFDLAAHQTASTKSQEIFDEE
                     LKTAAAKLTDEQKEALIQKRLAAEAANTKGEPMPEAAVRAWFERLFKGSYVPHVIEPS
                     AGLDRMALAVIAKAFSETEKVDEKGKKEVITVLRLHPRVAPIKVGVFPLLKNKPELVA
                     KAREVYALLKKHLNCFYDETASIGRRYARQDEVGTPFGVTIDFETCGEKGPELADTVT
                     LRHRDSGEQERVKISELLGKLLPLVS"
     gene            35516..35683
                     /locus_tag="PLP58_04010"
     CDS             35516..35683
                     /locus_tag="PLP58_04010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ07707.1"
                     /translation="MSTVIEIEKAIEALPQEELGKLREWFNEREATLVASASTFALYD
                     GEEGEAQQWED"
     gene            35689..35994
                     /locus_tag="PLP58_04015"
     CDS             35689..35994
                     /locus_tag="PLP58_04015"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014505.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004521 - endoribonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HRJ07708.1"
                     /translation="MRGEIWLVDLGMTQKTRPVLILSVAYKGEERALVSYVARTTSLR
                     GTEYEVPHQAAKFLPGAFDVQSIGTVPDVQLVRRLTVCDAGTLAKVEDALKRWLALR"
     gene            36109..36561
                     /gene="tnpA"
                     /locus_tag="PLP58_04020"
     CDS             36109..36561
                     /gene="tnpA"
                     /locus_tag="PLP58_04020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009959465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
                     /protein_id="HRJ07709.1"
                     /translation="MPQSLSLVIVHLVFSTKDRLPRFDAPLRPHLHAYLAEVARNAGC
                     EAYRVGCVADHVHLAIRLSRTLTIADLVETLKTSSSKWLKTQSAALSDFAWQRGYGCF
                     SVAPADLMAVTTYIDNQEEHHRTRTFQEEYRAFLNKYGVTYDEAYVWD"
     gene            complement(37040..37831)
                     /locus_tag="PLP58_04025"
     CDS             complement(37040..37831)
                     /locus_tag="PLP58_04025"
                     /inference="COORDINATES: protein motif:HMM:NF024206.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HRJ07710.1"
                     /translation="MFSALKRLILGSKTKEPEQRVPMVETCADCGVRSGELHDLFCTK
                     ERCPFCGGQLASCECIGSVLKLTHEEQQLVDEYIDDSIPPLCEIMQRWRDALTAKGRF
                     PFEAHLDDPIRAAYRGDFEAVRTFLDNGIDPDVGNQVGYTALMGAARGASVDVLRLLL
                     SRGGRARTTDKRGCSALHWAVAQSGDSSQTACVRVLIDAGADINARDEGGTTPLMNAA
                     WFGCMDAVRELLQRGADSRLRDNKERTAKDLASERGHSEIAALLA"
     gene            complement(37888..38232)
                     /locus_tag="PLP58_04030"
     CDS             complement(37888..38232)
                     /locus_tag="PLP58_04030"
                     /inference="COORDINATES: protein motif:HMM:NF024248.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HRJ07711.1"
                     /translation="MSTPAAPPTIPAPPPQVIPADTVYAALGDPTRRRLLQILADGQG
                     RTATVLAGNVGKRLDATLKHLVALRGAGLVVTAENPQDGRRLLYHLAPAIPATKTERG
                     WEMDFGYFLVRC"
     gene            38665..>39026
                     /locus_tag="PLP58_04035"
     CDS             38665..>39026
                     /locus_tag="PLP58_04035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ07712.1"
                     /translation="MTVKFPSSFRARLIVTIFPVVAGIALGALLLAEWKFAATYQRLF
                     EEQFESQIAAVAEAKARRFEALSSELERLALAPALVAAVKGGDFSGAMQELRPVLEAL
                     ATSRLQSEFPGAPLPRASG"
BASE COUNT         7734 a        12343 c        12045 g         6904 t
ORIGIN      
        1 gatgtggagc aggagccggc gaacatccgc gagatgtatg gcgacacgct tcagggccgc
       61 cagatcctga tggcgcgccg cctggccgag cgcggagtgc gctatgtgca ggtctatcac
      121 ggcgccggcc agccctggga cagccacgcc agcctggagg aaaatcaccg ccgcctcgcc
      181 cgcgagtgcg accaggccat cggcgcgctt ttgcaggacc tcaaggagcg cggcatgctc
      241 caggacacgc tggtcatctg gggcggcgag atgggccgca cgcccacggc gcagctcccg
      301 ctcacgccga agataggccg cgaccatcac acggacggct tcaccgtctg gatggcgggc
      361 ggtggtgtca aaggaggcta tacccacggt gtcaccgacg acatcggcct cagcattgcg
      421 gaggacaaag tgcatgtgca tgacctgcac gccaccatcc tgcacctgct cggctttgac
      481 cacgaggcgc tcacctatcg ttatgcgggc cgcgactttc gtctcacgga tgtctttggc
      541 aacgtggtga gggagatcct ggcttgacgc ccgcttaccg ctccgggctt tcccggtgca
      601 aattccgcgt tacccgcaac cctcccccgc gttgagtgtt cccgtcacgc cgatgaaaca
      661 cgccgccctt ttttccgcca tccttttttc cgccgcgccg ctggtcgcgc agaccacgct
      721 gatggagcag cgctttgacg cagtggacaa aaacaaggac ggcatcgtca ccgaggccga
      781 gttgccggtg aaaatgtggt tccgccttct cgatgccaac aacgacggcc agatcacccg
      841 cgcggaggcc atcgccggag cctcccgtct gcgcggagcg cgcggcatgg gcgcggcggc
      901 tgaggagcct gccgaaccgg aggacgcctc gctcaaagag gcgccgcgcg tgctcaaggc
      961 cgccgagcac ggagtcggcc acctcgtgcc cgacctgtct ctgcgcgacc tcgccggtca
     1021 ggagaaaaaa ctcagcgctc atctggcgga cgcgcgcggt ctggcgctga ttttcttcgg
     1081 cgccacctgc cccatcagcc agaaatacgg cccggagatg tcgcggctgg aaaaggacgc
     1141 cgccgcgcac gggctgcgtg tcattctcgt ctgcccggtg ggcgtcgaga ccacggacta
     1201 catccgccag ttcatctccg gccacggttt gaaatccccc gtcgtccacg acaccacggg
     1261 cgctctcgcc gccacgctcg ccgccacgac caccacggaa tgcttcctgc tcgatgcccg
     1321 ccgcacgctt gtgtatcgcg gcgccatcaa tgaccagtac ggcctcggtt acgcgaagga
     1381 acagcccacc cgcgcctacc tgcgcgatgc cgcagccgcc ctgcttcgcg gtgagccgcc
     1441 tgaaatcgcg gccaccagcg ctccgggttg cgcgctcgat gtgaaaaccg ccgcgccggc
     1501 agccacgggc gtcacttatc ataaccagat ctcccgcatc atgcaggctc actgcgttga
     1561 gtgccaccgc aaggatggcg tggccccctt cagcctggag acgtatgacg acctcgtcga
     1621 aaacgccggc atgatccgca agcaggtcga gcgcggcgtc atgccgccct ggttcgccgc
     1681 cccacccgcg gaaggccacg ccagcccgtg ggcgaacgac acttctctct ccgcgcggga
     1741 cagggccgac ctgctcgcct ggctcgccag cgaccgcccg cccggtgatc cggcggacgc
     1801 gcccctgccg cgcaactttc ccgcagagtg gttgctgggc aaaccggagg tcgtggtcca
     1861 gctcccccag cctgtcgcca tcaaggctga gggcaccatg ccctaccagt tcgtcaccgt
     1921 gcccaccgcc ttcgccgagg accgctgggt gagcgcctat gatatcctgc ccacagaccg
     1981 cagtgtggtg catcacgtca tcgtccaggt gcatgaaaaa ggtgcgcgcg tgcggaacag
     2041 gggcgagggc agcgagggct actgggccgc ctatgtgccc ggaaacaccc accaccgcta
     2101 ccccgccggc tttgccaaaa agctgcccgc cggggccact gtcagcttcc agattcacta
     2161 cacgccgaat ggcaaaaaga cccaggatca gctccggctc ggcctgcact tcgccaaaga
     2221 gcctccgcgc tatgtggtgc ataccgccgc cgtggcccat ccccggctga gcatcccgcc
     2281 gcacaccgcg cgccatgtcg aggtgaagga gcagaccgtg ccggcgaaca tgaacctcac
     2341 tgccctcatg gcgcacatgc atgtgcgcgg ggctgccttt aaattcgagg ccactcgtcc
     2401 tgacggcacg cacgaggtgc tgctcgacat cccgcgctac gacttcaact ggcagctccg
     2461 ctacgactac gcgcagccgc atttcctgcc cgccggcagc cgtgtgaagc tcaccgccgt
     2521 cttcgacaac agcgagtcca accccgccaa ccccgacccc tccaagactg tccgctgggg
     2581 gccgcagacg catgacgaga tgatgatcgg ctacttcgag tacttcacgc cgcgcaagga
     2641 cgatgtggcg atggagtaga gactgctctc gaggcctcac cttccccctt gcatccacag
     2701 caacttggcg cttgggattg ccgcgatgcc caagtatatc gtcaccatcg gcctggaggt
     2761 ccacgcccag ctcaccaccc gcagcaagat gttttgcggc tgcgccatcg cctacggcga
     2821 ggagccgaac actctgacat gccccacctg cctgggcctg cccggcgcgc tgcctgtgct
     2881 caatcaaaac gccatcgaaa aaaccatcct cgcgggtctc atgctcggct gcgaaacccc
     2941 tcctgtcgtg aagtgggacc gcaaaaacta cttctacccg gacatgccca agaactacca
     3001 gatcacccag ttcgatctgc ccctgtgcct gggcggcggc gtgcccttgt atgacctggc
     3061 ctatccgaag gacgctcaga aaagcatcgc caatcccggc aaagtggtgc agctcacccg
     3121 catccacctg gaggaggacg tggccaaaag cacccaccac gccggcttca ccaccattga
     3181 cttcaaccgc gccggcactc cgctcatgga gattgtctcc gaacctgaca ttgccagcgc
     3241 cgaggaggcg tttgcctatc tcaccagcct gcgccagatc ctcgtctatg gcggcgtcag
     3301 cgacgccgac atggaaaagg ggcagatgcg gtgcgatgtg aacatctccg tgcggcccga
     3361 gggccaggag cagctcggcg ccaagatcga gctgaagaat ttgaactcca tgtccgccgt
     3421 ccgccgcgcc atcaagtatg aggccgcgcg tcagatggac tgcctggacc ggggtgaaaa
     3481 actcattcag tccacacgcc gctgggacga cgaccgcggc gagaccacgc tcatgcgcac
     3541 gaaggaggac gcgcacgatt accgctactt ccccgaccct gatctgctgc ctctgcgcac
     3601 gccggacatc ctggcccgcg tgcgcccgct ggtgcccgag ctgccccacg aaaagcgcgc
     3661 ccggtttgaa aaggactacg gctgctccgc ctacgacgcc ggtgtgcttg tcagtgaaaa
     3721 agcgctcgct gcctggtatg aggccgccat tgccgcccag cccggcgtgc ccgccaaaaa
     3781 aatcgccaac tgggtcatca acgacctgct cggcgtgctc aaagacagcg agggcggact
     3841 cgccgcctgc cctgtgcggc ccgcacagct tgccgcgctc gtcgccatcg tcgaggcggg
     3901 aaaaatctcc aacacacagg ccagggaggt ctttgccgaa atggccgcct ccggtgccga
     3961 tcccgccaaa gtcattcagg tcaaaggctt cgagcaagtc agcgacacgg gcgcgcttga
     4021 ggccattgtg gatcaaatcc tcgccgccaa cccggaaaaa gtcgccgaag tcaaaggcgg
     4081 taacgacaaa gccatgaact ggttcaccgg gcaggccatg aaagccagcc agggcaaggc
     4141 caaccccaaa ctcgtcaccg agatcgttcg cagtaaagtg ctgtcataaa atcggatggc
     4201 gcgtgttttt gtccgtgggt ttgaatgagc accatgcaaa gcccggcatt catcaagggc
     4261 caggctcttt gacggttagg gccaatgacc gtgccatcgc ttgaatggtc gaagcatcaa
     4321 cggcgcggcg gcgcgtctgc cgctcgttcc aatgcagcac aaggccgcga ccgtagccct
     4381 tcattggagc gaaaggcagg acacggattc gtttccggtc cattccgcgc agaccgagcg
     4441 ctggcagtac ggctgcgcat tcgcccatgt ccacgagctg gcgcgcctgc aggatggaac
     4501 cgcactcgaa gcgtgggcgg tagctgaggc cggcgtcagt catcgcggcc cggatggcca
     4561 gatcgagctg gccgccatcc cgccccgctg caaagggcaa gtcgcgccag aaggagggat
     4621 gggatgcctg gcggtcgctc acgccctgcg ggatgagcct ctccgggatg cagatcacaa
     4681 atcccagctt taccagcggc agcacggact tcgctgcggg aatggcatcg cgcctgagaa
     4741 tggcgaagtc cgcgcgcccc tccgagaccg cctccgccag cgagcggctg cgcattggct
     4801 ggatgagcag cgtggctcct cccagaagct ccgccagtct gggcagggcg ggcaccacaa
     4861 gccactccag cacgctggca ccggctccga ttgtgaatga ttttggcagg ccgcgctggt
     4921 cgcggcggaa gtcctccaag tcttgaagct gctcccggat cagcgaggcc agtctcgcgc
     4981 ccgccggaga aagcgagagc gtgcgtccct tgcgccgtgt cagctccaca tcgaaaaact
     5041 cctccagctc ccggatttgc cggctgatct ggctctggcg gttcgcatca tcaggcgcgg
     5101 cgcgggcgat gcttcccgcc tcggccatgc tcatgaagcc gcgcagcctt tccagggtga
     5161 ggccgccttt ggaaaacaat ttttcaaaca tgacttttga gtcatgcatc ctgtggattc
     5221 agttattttg caagcgcgcg catgagccat gatggggcgt catgaaacaa caaagctacg
     5281 accttattgg tgacattcac ggccagcatg aaaagctggc gcggctcctg gagaatctcg
     5341 gctaccgtcc acgccctggc ggcggcatgc gtcatcctga cgggcgcagg gtggtctttt
     5401 tgggcgacta cattgaccgg ggaccggaca tccggcgggt gctggagacg gtgcgcggca
     5461 tggtggaaag cggcgacgcg ctcgccatca tgggcaacca cgaatacaac gccgtgcttt
     5521 atgccacacc ggacggcaac ggcggccatc tcaaggagca ctcggcgcgc aacacgaaaa
     5581 ctcaccaggc cacgctcgac cagtttgccg ggcggcccgc cgagtgggcg gagtggctgc
     5641 tatggatgcg cggactgccg atgtttctcg atctcggcgc tctgcgcgcc gtccatgcct
     5701 gctgggatgc gcgccgcata agccggctgg gcggcgccag cctgctcgac ccggatttcc
     5761 tgcgcgcaag cgccaccaag ctcacgccgg aacaccgcgc ggtcgaaaat gtgctcaagg
     5821 ggcccgagat gctcatgccg gagggatcgt gctttcacga caaggaaggc gccgcgcgcc
     5881 gcacggtgcg tgtccgctgg tggaacattc cgccgcaatc ccatgtttgc gctctggcga
     5941 tgccggaacc cttcgactct ccaggcgatg ccgctcctca cgacctgcgc cggctgccct
     6001 gttacggaag caatgagccg cccgtgtttt tcggccacta ctggctgccg cccgaaaaag
     6061 aaaaggcgcc cctcgcccgc aatctggcct gcctcgactt cagcggcgca cgccaggggc
     6121 cgctcgtggc ctaccgctgg gacggggagc gcgagctgag tccggaaaaa ttcttgtgcg
     6181 cctctctgct cgaaacaacc acgcacactc catgaaaaca cacacttcat tcgaagcctt
     6241 cctcaccgcc gcgcgcgaaa acgcgctccg aatgctgctc aacgccgaat acatccggcg
     6301 ggagctgccc tcgctgcaag tccccgaggg cttgcgcgct gacatcctcg aactgtgtga
     6361 tgactggtgt gaggccaagc atgatgcctt cagtctcatc tttgacatct ccgatatcca
     6421 tgctgaaggg gcggatattc gccagcattg cgcgcgcctt ttgtcatggc tgacccaggc
     6481 gtccatgaaa gcccatgccg tcatcatcca ggctcaggac tccgccgcct cttcgctcgt
     6541 cacgctcctg gttacagagt ccgctgtcaa tgtgctcaac gccaactccg ccgcgcatga
     6601 agcctgggcg gatcatctga acttttgaac tcccctccaa aatcaatccg cagcatgaac
     6661 cgcacccgcc gagccagaca aatccagacc attcaactcc tcaaaaccga actcgaaaaa
     6721 cccggcctca gcgcggaacg ccgccaggaa gtgagcgagt tcctcatgga ttgcgcgcat
     6781 gattgcttca tggacgacat gttcgaaccg gacttcttga acggaatcat cctcatccga
     6841 cacggcgaga gcatggcgaa tgcgggggaa cgcacacgga cgccgtccgg catccctctc
     6901 agcccgctgg ggcgggagca ggcgcgggat ctggagcacg ccgccttgga ccccgagctg
     6961 atcgtcgtct cggcctacct gcgcacccag gagacggccg caccgctttg ccaaagactc
     7021 agcgatgtgc ccgtggagac ctggcccgtg catgagttca cctacctggc tcccgaacac
     7081 tacctgaaca ccactgagca agaccgtcat gcaccagtcg cgcgctattg ggagcgcgcg
     7141 gacccgcagc atcgggatgg acctggcgcg gagacattcg ccgaattcat cgcgcgggtg
     7201 gatgccatcc tggagcggct gcgaagcatg gatgacccgc aagtatgcat cttcacccac
     7261 agctttttca tcttggcgct actctggcgg caaatgagac ccggtgcggt ggtggacgag
     7321 cgtttcatgc gtgagtacga catctttcgc cgggcagcca gaattgagca tgcgcgtcct
     7381 attccatttc gcatcatcaa atcatgatgc gttgatgcga gtggttgacc cttcgcgccc
     7441 tttgggtagt ctccgaccat gcccgcccgt cccaactgcc gtcctgaact cgaagccgcc
     7501 ctgcgccagc gcatcctggt gatcgatggc gcgatgggga cgacgatccg gcagtataag
     7561 gccaagggcg tgctcgatga ggcctcggca cgaggcgaac gcttccgcga aaacgagaag
     7621 gacatcctca acaacggcga catcctttcg gtgacgcgcc cggacatcat cgaggacatc
     7681 cacaagcgct tcctcgaagc cggcgcggac atcatcgaga cgaacacctt ctccgccacg
     7741 agcatcgcgc aggcggaatt cttccgtgaa gggaagaaga acccggaatt cttccagggc
     7801 atcatcgagg acacgttttt gaacgacctc gcgtgggaga tcaatttcga gtcggccaag
     7861 cagtgccgca aatgggccga catcgtcgcc gagcagacgg gacggaagcg ctacgtcgcg
     7921 ggggccatcg ggccgctcac ggtgtcgctc tcgcagttcc cggacttgag cgatttgagc
     7981 ttccgctacg tcaccttcga ccaggtgaag caggcgtata agcatcaggt gcgcgccttg
     8041 atcgccggtg gcgtcgatac gttgctcgtt gagacgattt tcgattcgtt gaacgcgaaa
     8101 acggcgctcg tggcgatccg cgaggtcttt gaagaggaca aactcgagct gccggtgcag
     8161 atcagcgccg ccgtcggccc gggtggtgag acgatgatct ccgggcaggt cacggaggct
     8221 tatttgaatg ccatgcggca tgtgaagccg ctctcgatcg gcctgaactg ctcgctcggt
     8281 cctgacaaga tgcgtccctt cctcgcggaa ctcgcggcta aggcggattg cttcgtctcg
     8341 tgctacccga acgctggcat gccgaacccg ctcgcgccca cgggcttcga tttgctgccg
     8401 ccggacatgg ccggttacgc caccgacttc gccagcagcg gcttcgtgaa catcatgggc
     8461 ggttgctgcg gcaacacgcc cgagcacatc gccgccatcg cgaaggcggt ggaaagattc
     8521 gctccccgtg aggtgcctga tgatagccat gacatgcgtc tgagcggttc gcagccgttt
     8581 gtgctgaagt catggggcac agcacgggga acaggcgtct cgcctgttca aacagagggc
     8641 aggcaggatg cccgcccccc atacctcatg atcggcgagc gcacgaacgt ggccggctcg
     8701 ccgaaattcg cgaagctcat caaggaaaac aaactcgagg aagccgtcgc catcgcgcgt
     8761 cagcaggtcg agagcggcgc gaacgtcatc gacgtctgca tggacgaggg cctcatcgac
     8821 ggcgtgccga tgatgacgaa gtttttgacg ctcctgcaaa ccgagccgga ggtgaacaag
     8881 gtgcccatca tggtcgattc ctcgaagtgg gagatcatcg aggccggtct gaagtgcctg
     8941 caaggcaagg gcatcgtgaa ctccatctcg ctgaaggaag gcgaggcgaa gttcaaagaa
     9001 tacgcgaaca agatcaaaca atacggcgcc gccaccgtcg tgatggcctt cgacgagcaa
     9061 ggccaggccg cgacttacga agacaagatc cgcatctgcg agcgtgcgta tcgcatcctc
     9121 gtcgatgagg tcggtttccc gcccgaggac atcattttcg atccaaacat cctcaccgtc
     9181 gccaccggca tcgaggagca caacaactac gccgtcgatt tcatcgaggc cacgcgctgg
     9241 atcaaagcga acctccctca cgcgaaggtc agcggcggcg tcagcaacat cagcttcagc
     9301 ttccgcggca acaacaaggt gcgcgaggcc atgcacagcg tgttccttta tcacgccatc
     9361 aaagcgggca tggacatggg catcgtgaat gccgggatgc tcgaagttta cgaggaaatc
     9421 ccgcccgaga tgctcgtgaa ggtcgaggac gtgatcctaa accgcaacaa agaggccacc
     9481 gaggtgctcg tcgattacgc cgagcagttc aaaggccagg ccggcagcgc gaagaaggcc
     9541 gaaatcgatc tcagttggcg tgaagccagc gtcgagaagc gactcgagca cgcgctgctc
     9601 aaaggcatca ccgatttcat caacgaagac accgccgagg cgctcgcgaa gctcggcaag
     9661 ccgctcagcg tcatcgaagg cccgctcatg gacggcatga gcgtcgtcgg cgacctcttc
     9721 ggcgctggga agatgttcct gccgcaggtc gtgaaaagcg cccgcgtgat gaagcagagc
     9781 gtcgcgtatt tgcagcctta catggaggcc gaaaaagccc gcaaggcccg cgaacgcgag
     9841 ctgctcatcg agatcgcgga gaagacggtg aaggcactcg aaagcggcag tgacgtgacc
     9901 gccgtcgaag gcttcaccta cacgccctat cccggcctca gcctcgaaga gcgccgcatc
     9961 gaggtgaaat tcgccgccga gctcgccgcc gacctcgaag ccgccgccac ggaatacaaa
    10021 cgccgctttg ccaacgtcct cgaccgcaac aacgtccagg aactcagccc cgactacgcc
    10081 gccacccgcg aaagccgcca aaaatggagc atcgccacgc ttgagcctgc gggtgcgttt
    10141 gtggactggt tctttgccaa agtgctcagc acggcgcaat ccaaagacac tatcatcttc
    10201 aatgccggcg gtcagggcag cggcaaaacg acggccacgg ccgacatcaa ggtcactggc
    10261 aacgccatcc tcgtgataga cggcacactg caaaaccacg cccgctccgt ggagcacttc
    10321 cgccccgctt tcgccgccgg atgctccatc gagctgcgct ttgtctattg caactggccg
    10381 gatgcggtca ggaacatggc gggccgtgcc gcgaaaggcg cggggcgtgt ggtgcctctc
    10441 aaacgagccg ccaaaggcca tttcgaagcg gcccgcacca cgctcaagct ttgcggtgag
    10501 tttgccgaca aaccggaact cgacgtgttt gtcgtcgaga accgcgtcgg cgctcttccg
    10561 cgctccttga acctcgcgtg gctctccgag cacctgcaca attccgttga ggaactcctt
    10621 caaaccggcc aaactaccct ccaagatgaa ttcaagctcc actccgacga tcccgactac
    10681 agcgcccgac tcctggcgca atttcaagcc acgtctgagc caggaagtgg cggagcggtt
    10741 tcgtcagatc gaggaaggca cgttggaggc tctgagccgg gcctcgggtc tgggcaaccg
    10801 ggtgatggaa aagccagcgg aagcaccgct gcctcgtccc tgaccgccgc cgatttgatc
    10861 cctgccgagg cggcgaagca gggtggtggc aaaatcgtgc tcgccaccgt gaagggcgat
    10921 gtgcatgaca ttggcaaaaa cattgtcggc atcgttttgg cctgcaatgg cttcgaagtc
    10981 accgacatgg gcgtcatggt gccgtgccag aagattctcg acaaagccgt cgaagtcggt
    11041 gccgatgtca tcggcctcag cggcctcatc acgccctcgc tcgatgaaat ggtgcatgtc
    11101 gcggcggaaa tggagcgcct gggcttcaag cagccgcttt tgatcggcgg agccaccacc
    11161 agcgccgcgc acaccgccat caagatcgcg ccgaaataca gcggcagcat cgttcacgtg
    11221 ctcgatgcct cccgcagcgt gccggtgacc accgcgctgc tttccgaaga tcaacgcgaa
    11281 ggcttcatca agtccaacga agagcgccac gccaaactcc gcgaggaata cggccgcaaa
    11341 aaagatcgcg agctgctcag cctcgctgaa tcacgcgaga agggccaaaa attcgactgg
    11401 agcacgcagg acatcgccac gccgagtttc actggcacga aggtctatga aggcgctgac
    11461 ttgatctcca cactgcgcga gttcatcgac tggtcgccct tcttccactc gtgggagctg
    11521 cgcggacgct ggatcgcgaa tgaaggccgt ttttcctccg cgcatgaaga tgccgaaatg
    11581 aagctaaaag ccgaagcgga ggctttgaag ctctacaacg acgccaacac gctcctcgac
    11641 cgcatcatcg cggagcagcg cttcaccgcc aaaggcgttt tcggcctctt ccccgccaac
    11701 agcgtcggcg acgacatcga agtctatgaa ggaggaaagc tcaaaacgac cttccacacg
    11761 ctgcgccagc aggtcatcaa gaaggacaag gccaacttcg ccctcagcga ctacgtcgcg
    11821 ccgaaatcca gcggccgcac cgatcacatc ggcggcttct gcgtcggcat tcatggtgcc
    11881 gatgagttcg cgaaagagtt cgacgccgcg cacgatccct accacggcat tttgaccaaa
    11941 gccgtcgccg accgcctcgc cgaggccttc gccgagtatc tgcatcagca agcccgcttc
    12001 gcctggggct acgaaaagcc cggcgacctc tcgatggagg acaccgtgaa ggaaaaatac
    12061 cgaggcatcc gtcccgcgcc cggttatccc gcccagcccg accacaccga gaagcccatc
    12121 ctcttcgccc tgctcgacgc caccgccaaa accggcgtcg aactcaccga aagcatggcc
    12181 atgcaccccg gcagcgccgt cagcggcctc tacttcgccc accccgagtc tcactacttc
    12241 ggcatcagcg tcctcggcaa agaccaagtc gaagactacg cgaagcgcaa aggcatgagc
    12301 gtcgaagaaa tggagcgctg gctcggccct tggctcgggt attgacgcga aagggcggtt
    12361 gctcactccc ccggcggctt gcggatttgc cattgcttct ccggccacgc caggttcccc
    12421 gccaccgtgc tggggaactc ccatggcgtc tcgtccgccg tgtggagcac cacgcgcgtc
    12481 ttcgaggcgg ccaaagcggc ggcctgcggc atgtcgagaa ttttcagcac attcaggtag
    12541 gcaggcccct gcatgtgcgt cgttggcagg tcgtggaggt ccaggcgcgt gatgccctcc
    12601 tcgaagagcg ccgcatacag cgtgtttgcc gccatgtccc ggtgcgcctg cagccacagg
    12661 ggtgtctcct tcagcccgct gatctcccgc accgcctgga tggcgcgccg gatgtcccac
    12721 acgcgcatcg aatccagcgt ctgccccgcc aggtaaaacc gccgcagcct ctgcgtttgc
    12781 gctttctcgc tccccgtcca ttccgtcggg ccgatgccgc gcgggcagag gtaagccatg
    12841 ccccacttga agttttcaaa catcttccgc tcgctggtaa aggcggtctc gtccgcctcg
    12901 ccctgcggga aggaatcgaa gagcttcgca aagcgtgact cataggtgtt gcagaaatcc
    12961 tgccagccct gctcgtcgag cacattgagc gccaccagct ccagatcctc cagccgcagc
    13021 cccgcgcggt gcgtgatgtg cagccgcagg cggaaaggct gctgcgtcac caggtcaaaa
    13081 acgctcatct ggatgccgtc ccgctccatg ccgccctcct tgaccggctg gatgccaggc
    13141 gcgtccttag gccagcctgc gaagaccttc tcccgcagcc cctgcatcca cttcgcgctc
    13201 tgagcctccc actcccccgc attcgtcggc ggcgcgggcg cggcggcctg cggcacaaag
    13261 gtctcgtcaa tcgtcgtgtt gcgctcgtcc gccggagtct ccgccagcac gcgcagggcc
    13321 gccgggtcca ggcacttttc cgcgccctgg tgcagcacct gcatcgcgtc cgcgcctttc
    13381 aggtgccgct cgaaccagtg aaaagcgccc atgttcagcg gctgaaggtc cgaatgcgga
    13441 ccctcagcca cttgcagccc gatgtttttc tcctgcccca gcagcgaata cagccggcgc
    13501 gtgttttgat aaacctggaa gacgccgtca atcgggaaga tgctgtcctt gtccgtgttc
    13561 acgatcagca gcgggcgcgg cgccaccagc gccgccaggc ggtcaaagtc ccagcggtat
    13621 gtgttcacga aatacatgca gtcgcagtgc ccttccacgc agccgtccac cacatgattc
    13681 ttcagcgagg tcaccccggc ggtcggagcc gcggccttga tgcgctcatc cagcgccgcc
    13741 acccaccacg aatacgctcc gccgccgctg cggcccgtca ccccgaagcg tgtcttgtcc
    13801 acctccaggc gcgtctcgag aaaatccagc gcgcgcatgc ccgcccacgc ctccagcccc
    13861 gcaggtgtgt aaccgcgcga gaaccaccac cagcgcccct tgctgtaagt gccgtgatgc
    13921 tctccgcgga tctcaccgag ctgcaccgtg tcaatgatca ggcacacata cccgtgccgg
    13981 gcgaaccaca ccccgtggtg gtgatacccc gtcttgttgc ccaggctcac cccgtccttc
    14041 accacattcg catggccgca aacatagagg atcgccgggc agggcgcctc caccttttcc
    14101 ggcagataca aattcgccgt cacataatac cccggcacag cctggaaatg catcttctcc
    14161 acgcggaagc cctccccctt cagctccccc gtcttcaccg gcagcagcgg cgtccgctcc
    14221 ggcatcggct ccaggcccag catctccgcc agttgccgcc ggtactccgg ctcatgttcc
    14281 cgccactgct ccgccgagcc gatgtgcttc agcccgcctg tctcctcgat ctcgcgcact
    14341 tgctgctgga agtagcggtc cagcatccgt cttcccggcg aatccggcgt cgcctccgga
    14401 aagcgggccg agacaggcag agcggcgagc agcaggagca gggcggagaa tttcatccca
    14461 caccatacag agcgcgcggg gcaggcatat cagggaaagc tggccgggga gtggcgaggg
    14521 aataagcagc gcgtgccccg gccaggcgct gggagcggtg cgtccttggc ggcagactcc
    14581 ttcatggcag tgaaaaaaaa ccgccaccac cctgcccgcc ccggttcaga agcaacccgc
    14641 attcccggca aggagcggac cccgccgaca cgaaaaatct tttttgatcc agcgcggaac
    14701 tcgccgacag aaaaaccagg tgacgcgcgg ttttattcgg cgagccaccc gccggtgaag
    14761 gcaggtgtgg cgccgaggtc ggcgagccag cgctcccaga ggatggcgcg ctcgcgctcg
    14821 ctgagcacgt tgccgcgcca gcaggctgcg aggacgaggc gtccatgctt ttcgttgacg
    14881 aagaggccgg tgccgggcgg ggaggtaaag ccggggatgt ggcaggcggt gcgcacgggc
    14941 gcgccgagga aggcggtgag accgggggcg aactcgccgg tgtcggagtg gaagaagctg
    15001 ttgaagaggc ccttcatgcg ccaactgacg aacttcatgt agatgccggg gggcacgggg
    15061 cgcatgacgt tcatgagctc gtcgaaggct ttgtccagcc cgtccttgag gtagcgctgg
    15121 tgctgggcgg cgatgctgga gacggcgccc tccagggtgc gcagctcctc gcgcaggcac
    15181 tggccaaaga acatgcgcag gtggttctgg aagatggggc cggcggcgcc ggggcggcgg
    15241 gtctggatgg ggacgctgca cagcaggctc tccggcacgc cgccgcggtg ctggaagacg
    15301 gcccggtgag cgcgcatggc gcaggcgagc tggaagggga ggttcagcac ttcggagaag
    15361 gagcgggagc gggctttgac ggcggcggtt tgcgcgtcgt ccagcaggcg gacctcgtag
    15421 cggatggggc cgctttggcc gcctttgcgc ggggccaggg agtcgaaccg gtgcttggtg
    15481 atctcctcga agcggccggt gatggggaag gtggcctgcc atttctgtcc gtagtggcgc
    15541 gggtctggct ggggtgcggc ggtgtcgagc gcggtgatgg cggtggcgcc ggcgctgccg
    15601 aggtcgtgga gcgcctggag gaatagctcg gcgcctttgc cgtccacgac gaagtggctc
    15661 cagctcagca tgagggtggc ggtgccgttc cggcgctcga cgagggcgag gcgcatcttt
    15721 acccagcggg cgtcatcccg gcaggggatg ggggtgttga tgaggctggc gatctcggcg
    15781 tcggcgtcgg cgcagggctg cgcgccgggc agggcgccgg gggagccatt ttcgctgatg
    15841 aggaggaggt ccggcggcag gcgctccagg ccggccaccc agtggtgcgg gctgaaaaga
    15901 ccggtcttct ggaggcgggc ggagagcagg gggtagcggt ccgcggccag ctccagcgcg
    15961 cggcgcaggg cgggcacatc gggcacggcg tccagctcca tgagggagtg ggccacatgg
    16021 ccgccctcgc ccttggcgcg catcatggcg tcgaatgaga ggacgaagta gtcgcagttc
    16081 ttcaggggca gagtggtgaa ggtctgcgcg ggcgcgtcct ggacgggggc gggcttttct
    16141 ccggcgggcg gctctggcgc ggcgggattg gcgggctgcg cgacggcggg cggctcaatg
    16201 ccgaggcggg aggcgacgag gagtgccacg ccatgcacgg tgcggaagtt ttcccgcgtg
    16261 acggtctcga cggggatggg ggagccgaac tcctcctcaa gcaggaggag gagctgcatg
    16321 atggccatgg agtcgaggcc ggcgtcgaag aggtcgctgc tgtcttgaaa atcagggccg
    16381 gtggcgagca ggtccgcgca gatgatgcgg cgcgcgcggg tgaggagatc ggaaaaatcg
    16441 ggtgcgggca ttgcgggaaa tcagagggcc gggagtgccg gggaagggga gagggtttca
    16501 gcggcgcgcc gcccggacag gcggcccagt tcgaagcagg cgcggccggt tttttcacgg
    16561 acgaggatgc cggggcgggg cgaggcggtg cggaagcgct ccagggtctt gccagcttcg
    16621 gcgaactttt caagccgcag ctcgtgcagt tcggccatga ccacatcatg ggcgagcgcg
    16681 aaggcgggga tgaggccgat ccggtcggcg gagggggtgt ccttgactcc gacatcatgc
    16741 acaagcacac gggagacggc ggcctcaccg agccagtgca tgaaaggggg gttttgcgcg
    16801 atgccgccat cccagagcgc ttcgccgccg atgatctggt gggagatgac gccggggaag
    16861 gcgcaactcg ccacgaccgt ctcggccagc ggcccccggg tgcggatctc ggcgcgccgg
    16921 gagctgagat tggtgaccgc gagaccaagg cgcggcgcgc agtcctcgat gcggcggccg
    16981 ccgaagatgc cgctcaagta gcgcaggatg gcgcgtccct ggatgccgcc ggtgtgcttg
    17041 cgtttgtgga cgggtaaaaa cgcgccgccg tagagcatgc cccactcgaa gaaggaccag
    17101 cggaagcggc gcgtctgcag cagctcaaag atctccagcg gactcatgcc ggcggcggcc
    17161 aaagccgcca ccagcgcccc ggaggacgcg ccagcgatct gccccggcca caccccgcct
    17221 tccacgaggc cctggaggaa ccccgcgtgg ctgtgaaagc caagaaaggc ggaactgagg
    17281 gacacggcca catccggccg cgcatcgggg agattttgca tgggcgcgga tgctagcgcg
    17341 cgcgcacccg gcggcaactg tgcggagatg cgcagaatta cagatttcct tcgatgaaag
    17401 atttcaccgc ctccgcctcg gcggcgagcg ggtgggtttg caggggcttg tctttgaggg
    17461 cctccaggct ggggtgcagg ggctcggcgc cggtggcgcg ggcgacgact tcggggaact
    17521 tcgccggact ggcggtgctg agcaccacgc tgacgcggtc cggcgccatg ccggtgaagg
    17581 cgcaggcggt gtgcgggtcc accacatagg cgtgctcttt ccacacccgg gcgatggttt
    17641 cctcaatcgc cgagtcatcc atgcgcgagg cgcggaagcc gaggtccact ttcggcaggg
    17701 tgacgtgctg cccggctttc atgcgctgca tgagttcgcg cacgcggagc tggtcgcctt
    17761 gaacgaggta gtaaaggtag cgttcgaagt tcgaggcggc ctggatgtcc atgctcggcg
    17821 cgtggctggg atgcacatcg ccctggcggt agtcgccgga ctcgaagaag cggcagagga
    17881 tgtcgttttg gttggtcgcc acgcggaagc cgccgcaggg catgcccatg cggtgggcca
    17941 gccagccggc gagcacgttg ccgaagttcc ccgtgggcac gacgaactcg gctgcggtac
    18001 gcgcctcttc cggcagccgc agccaagcgt ggatgtaata gacgcactgg gcgagcacgc
    18061 gggcaatgtt gatcgagttc acggctgaga ggttcacgcg ggcggtgaag tccgagtcgc
    18121 cgaagcagtc cttcaccacg cgctgggcgt catcgaaggt gccgggcacc gggatggcga
    18181 agacgttgtc cgcgccggtg caggccatct ggcgctcctg cagcggggcc acgcggccat
    18241 gggggtagag gatgaaaatg cggatgccgt cctggcccat gcagccgtga atggcggcgc
    18301 tgccggtgtc gccggaggtg gcgccgagca cggcgagctt tttgcccgtg agccgcacct
    18361 ggcgcttgta gagcaggccg aggagttgga gggcgaagtc tttgaaggcg agcgtgggcc
    18421 cgtgccagag ttcgagcaca taagttttgt catcgagctt gcgcaggggc gccacctccg
    18481 gcgagtcaaa gcgtgcataa gcctgccgcg tcagcgcccg ccattcctcc gccgggatgt
    18541 ctggcgcgaa gatctggaag aactccgccg ccagttccgc atacgccagc tccgcccagc
    18601 ccgcgagccg tccgctgatg tcgggcaaat gctccggcag aaacaggccg ccatcgggag
    18661 ccagtccggc ttcgacggct tccgtgaaag tgtgcgggcg tgtctgcccg cgcgtggaga
    18721 tgtagaacat gcctctccaa tgtgcggcgc tgcggaaaac tcaagcgcgg gtgtgctggc
    18781 tttggccgcc gcagggaacc gggcgggatg cccggttcac acgagacagg cggggacgcc
    18841 agtctccatg gctactccgc cttcgtctcg atgacctcgc caccgagagg cagcacgagc
    18901 ttgtcccgca cctgctcgaa ggagcgcccg cggatggagg cgtggatgta gagcttctgc
    18961 ttgcgggatt tgagcttctt gaacacctcc tccatgtgcg cctcgtccac gcggtcctgg
    19021 aagtcggcga accagtagat cgtgtcggcc tccatgactt ctttgcacat cagcgccggc
    19081 gaagtgtagg tgatgccgtt gaagtcaatg aaataggtgt ttgggcgctt gaccatctgc
    19141 gcgtagatcg cctccagcgg catcggtgtg ttcggatcat aaaccagctt tttgcgctcc
    19201 tcggccgtca tgcgcaggga ggctttgccc tgccagtgct gccagaatcg ggcaaactcg
    19261 tccccctggg ccttgcgcac cttgtcatcc atgtcgcgcg gcggcttctg cagcccgcag
    19321 ccgaagtaaa gcaccagcgg gctgccgaag gccaccttgt ccagctcctt cgccacgatg
    19381 ggcaggtagc gtgtcattga gcgggagaca tccatgacca cggcgatctt gcgcgcgtcc
    19441 ttcagctcca tgccgaacat catgatgggc ttgaaagtca cgccgcccat gccgccgcgc
    19501 cccagccccg tgccgaagcc gccaccggcg cccgccttgc caaagccgcc gctgcccagg
    19561 ctgccgccgc cgagcaggga ggacatgtcc ggcacgtcga gcaggtccag cggcgcctcg
    19621 ggcagggaaa tctcggaatt catgttcgtg gtgacgattt ttttcatcgg catcaccttg
    19681 ttgagctggc tgcgtttttt ctgctgcacc ttgtggcgca tctcggcgct tgccgccgcg
    19741 ccctgctggc tgccgccgcc gggcaggaag tccacctgct cctggatcat ctgcgtgctc
    19801 accacaatgg cgccggcaaa gagcaggatg ccgaggtgga tggccacgct cagcgtcagc
    19861 gagccgccgc caacggcgcg ccattttttg acaaaacggg accaaaggga gctgcgtgga
    19921 gtgtcttcat tcatggctgc gggcgggttg gggatcgaag tcgtgatact ggctggacga
    19981 accaaaaagc agtatctttg aaaacaggcc gttttggcaa tcccaatctg cattttttgt
    20041 caggccgccg tttccgccgg gcgcttccag atgggcacgc gcgttttgac cgcgtgcaga
    20101 taggagcggc acaagtcgaa ggcgggcgcg ctgtggcgcg cgcgcacccg gatgaggatg
    20161 gaaacctcgg ccgccgccac aaagcccaga cggtgctgga tgaagacctc atgcccgccg
    20221 tgcgcggcgc gcgcctccgc gatgatggct gccagcattt tctccgccat cggcacataa
    20281 gcgctgtact cgatgcccgc gatgacgcgc ccatcctcca gatcgcgcac aacgccgaga
    20341 aagcgcgcct cggctcccag gccgggaccg aaaggcggcg tggtgtcaga aatggggtcg
    20401 tgcgagagca ggtagtgcat gcatggattt cttcgccgca gagaccgtgc aagcaagccg
    20461 ttcgtcggag gtttgcgcct ggaatgccct ctgacacggt ccaaaaaacg gacggcaagc
    20521 tcatttggcg attgatagga cgcggcatgt ggcgaacaac atccgcgcat gagaaagaat
    20581 cccgtggacc aatcgcgtct ggccgcccag gtcatcctca atgtgcgcag cggacgcgcc
    20641 acttccaggc gcgggctggc ggaggcgctg agcgtgtcac cctccaccac aggcttgtat
    20701 gttgacgagc tggtcaaggc tggattgctc cgcgagaccg gactggagca gggaaaaatg
    20761 ggacgcccga aacgccggct gagcacggtg ccgggagcgg gttggtttgc gggtctcgaa
    20821 ttccacgccg ggcgcgtgca ggcggcgcgg gtggattttt cgggccgcct gatccattcg
    20881 cagagctttg cgctgccggc gaatccaact acccggacgg tgatggagca gttgcgcgag
    20941 gcggtggcat ggctcgcagg ccggtcgcct gggcggatgc tggccatcgg tgtcggcgcg
    21001 ccgggagtgg tggatccgaa aaatggaacg ggtctgcatt attcgttcat cgaagactgg
    21061 cgggatgtgc ctgtggcgga ggagctgggg ttcgaattcg gcgtgcgggt gacgcttgag
    21121 aacaatctgc gcgtcatcgc ccttgctgag cgctggttcg gtgacggtgg aggactcgcc
    21181 aattacatcg tgctaggccc gcgccggggc tttggcgttg ccattgtgaa tgaaggacag
    21241 cttctcggcg gcgctcacca agccgctggc gaggtgggaa actggtggtg gcccgctgga
    21301 ggcgcaatgc gcgagatgca tgatgaactc tcagccccgg ccgtctggcg gcgtcttgcc
    21361 ggcgcggctg aaactgaaaa attgcccgct gacctgcacc aagctctggc cttggtggct
    21421 cgaaactccg cgcgcgagga agtggtgcgc gacttcgcca aagtcaccgg ctggctgcaa
    21481 ctgctgctcg acacggaagc cttcattctc cacggcccgc tcaccgcgct cggcgattcc
    21541 ttctggaacg aagtttccga ggagaccgcc aggctcatgc caagactggc ggcccgccag
    21601 cccaggatca cttgctcttc gctcaccgac aacgcgggcg cgctcggcgc cgccagcctg
    21661 gccatggagg cctggcagcc ttccagttag gtcagccgcc gccagcgcat gcccagcgcc
    21721 acgctccatt ggtacttgcg cgcatgctcg cggatgagaa acggcatgtc cttctcaagc
    21781 tccagcgtga agcacgcgct gagccggtcc ttcaaccaat ctcgcgtgga ccctgcgggc
    21841 caattcgcgg cgggtgtgaa ctcctccagc caggtgcatg gcgtggctaa caatagctgt
    21901 cctcccggcc tgaccaaatc aggcagcctt tcaagaagcc tcgcagggtc gctcagccgg
    21961 cacagcagat ttgccgcgtg aactatgtca aaccctccca aacccgcgcg caggctcatc
    22021 gcatcgccct gctcgaaagc cgccgcctcc accctggcgc cctctggcaa agacgcgcgc
    22081 agcaacgtgc tcgcccgtcc ttcatcgagc cgttcataat ccaggcagcc ttcatgtgcc
    22141 agagtccggg ccgcacccac aaaactgagg gagtaatcca ggccaatcac ttcggcagcg
    22201 aagcgcgtca gctcaaatga cgagcgcccc accgcgcagc caaggtccag cgcgcgcgcg
    22261 cccggcggca aagcgtcctt gtccagcagc tcatgcaccg tacgccgcgc gaagttcagc
    22321 gcctcatgcg gcccgcgctc ccaaggcagc acctctttcg ccgcgccata gtgaaagagc
    22381 aggtattcat cgagaaggcg gcgagtctca taaatgttca tggaagccat gggtggagaa
    22441 agagtcacgc gcgcagtttg gcaagcggct tcctcatttt ggcgcgcaag tactgctgcg
    22501 ggagaagaat ttttcaggcc ggttcagcca tcagaaggat gctctaggag tgaggttctc
    22561 atcgcaaaca cccgccgata aaaaaatcta ttattttttg ttgacgcctc aaagaagtgt
    22621 ggcagcttct cgttaccttc aaaagtcatg aactcttatc gcacatcaca tcagaccatc
    22681 gcggcgctcg cagagcgtct tggggtggct ccatgtgcgg agtttaccgg cacggtcccg
    22741 agtttggtgg gacgcgattg gagaaacccc aaatccggag gtcaacgagc aggacactaa
    22801 agtccactgg cgcacacaca gcccttttag cttttcccag ccctgctcgc acctaccaaa
    22861 ggcggcaggg ctttttgttt tttcaaactc aaccccgttt tcatttccac ccagattgaa
    22921 tttcacaacc tagacggttg atcccccatc acagaaaacc ccggcatccg ttgacgcgga
    22981 tgccggggcg gcggccaaga cctggctgac tcccggagga gccctttcaa tgcatccaag
    23041 acgagacctg accacaggtc cggcatcctc cagagaagtc aagttcgagt cgccatcaat
    23101 gagtaggatt tcctaagcac taagtgcgga gatccaggga atcaccgcgc tccatttgac
    23161 atgtccagag gcttcccagg aggtgtgccg caaacaccga aacatccgcc gtgaaacatc
    23221 ccgcgggtgc gacgccagcg atgagcccac gctcatcccg cgcgtggcag cggtgttcgt
    23281 tcttttgtgg cgggtcatcc gggaaaccgg gtggccggcc atgaaagggc gcagagagct
    23341 ggtgcagttg gaacgggtcg gacgggtttg ggagaagccg ccgaatgtgg atgaccagcc
    23401 tctgcgccct ttcatggctg gataacaacc agacacagaa acggcggtgg cagacaagca
    23461 atgcgcagga ctccaaaccc tggaaatgtg ggggcagtac ccacccgccg tgccattttc
    23521 atagagtggt gcagccgtcc tcgactgcct tcgaacacag aacgcggcgg tagcccgctg
    23581 aagaggcgga ctggtctgta aaaccagcgc tcatcaagcc ggcacggagc attaccgtga
    23641 cgccgtacca ttttccaaat gcagggagag ccagtgctct gcccggtctc ataagccggg
    23701 cttggtgggt gcaactccca tccctgcgcc catttcaggt ccgttagctc agtccagcca
    23761 gagcgcccgc ttgtcgagcg gagggacgcg ggagcaaagc ccgcacggac cgccagtttt
    23821 caaagggctc gtggtgtcag aaggcagcac ggctgttttg cacacagcag gcgacggagc
    23881 gttaccgtcc gagtccacca gtttcaaaat ccccggtgat ccagcagtag gatgccgctc
    23941 tgttaaagcg gaagcgctgg tgcgacccca gcccggggag ccacttcaac cccaaccgcc
    24001 cgcaaggtgt aacggaactt tgcacacgcc gcgtctaacg gtggaggttc caggttcaaa
    24061 tcctggtgcg ggcgccattt tcaaaggagc acgtccggca tagacgagga aacggtctcg
    24121 aaaaccgctg cgccgctcat tggcgtccag agtgcgattc tctggtgctc cgccacttta
    24181 tttcatccct ctgtagctca acagcagagc actcgcccga taagcgagag accgtggagc
    24241 gtcaccacgc cgagggacca tttccaaaga acgctggatg gctcagacag atgagcggcg
    24301 ggttgaagac ccgcagaggt tggcgcgcta ccaactccag cggccatttt ttcacgcggc
    24361 attgatgtaa cagcaagcat gtctgtcttc caaacagagc gtgccggggc agcaccggca
    24421 tgccgcacca ttttcaatgc tctcaaagtg taagagccat gcacgccact ttgcgaaggt
    24481 ggaagttcga ggtgcaagtc cttgtgagag cgccatttca tgcggtcatc gttcaatagc
    24541 aggacgcggc catgccaacg ctgcaacgcc ggtgcaagcc ggctgaccgc accattttca
    24601 tgtcttcgtg gccgaaacag aaaggcacct gtcttgtaaa caggctcatg tgggcgcgaa
    24661 tcccaccgaa ggctccattt tcacgctccc ttcatagtgt aacagcagca ttccttgttg
    24721 tgaacgaggc ggtgccgggg cggaaccggc tgagggaacc atttcaatcg tgcgcacggc
    24781 gaagtcgtcg agccagtggt ttgcaagtcc acgcaacccg gtgcgagtcc ggggcgcacg
    24841 tccatttttt caatcggata cgagagagcc agcgaactcg cttgccttgg aagcaagagg
    24901 cgcccggggc agcaccgggg tatccgacca ttttcactgg tccgtagctc aacagcagag
    24961 caccaggctc ataacctgaa gggtgctggt gcaaatccag ccggaccgac caatcaagac
    25021 gcgaagagcg aagggcatag agcaaagagt ccaaaccaac gttgaagcac gcgtcagtgc
    25081 gaattggaac tcttcgctct acgccctttg ccctccgcct ccgtagccct gtagctcaat
    25141 gtgagagcat gcgcctttga agcgcacggt tgatggtgcg agtccatcca gggttgccat
    25201 ttttcatgcc cgcgtagtcc aacagcagag acaaccgtct cagaagcggt cgagtgcagg
    25261 tgcaactcct gccgtgggca ccatttcgat gctcctgtag cccaaagcag aggccgccgg
    25321 cttaaacccg gtgtcagtgt gagtgcgatc ctcaccggga gcacgttcac gcgcctgtgt
    25381 agtccaacag cagagacagc cgcctcaaaa ccggcgcagt gcgggtgcaa ctctcgccac
    25441 aggcaccatt catcgcgaag gacgacctgc aatgcactca gactctacgc tctttgccct
    25501 tcgctctaag cttcctcgct cccgtagctc aatgtataga gcgccgcgct tcgaacgcgg
    25561 aggctggagg cgaaagtcct tccgggagtg ccaatttaac gtagcacgag ttttccaact
    25621 cgtggctgaa cgagctggaa agttcgttct acaaaggaac gtcaaccgga ccagcgagcc
    25681 gggacttgtt gctaacaaga tcgtgccgcc accgcgcggc atgaggagca tgtcctcggc
    25741 gttccgccat tctttgccgt gacacagaca ctcctgtctg tgattcagcg ccacacaaaa
    25801 aaccacccac agccatgaaa cgcaaacttg cctccatcca gagcgtcctc gccatcgagc
    25861 cgattccgaa cgcggatgcc atcgagctcg tccgtatcca gggctggcag tgcgtggtca
    25921 aaaaaggcga gttccgtgtg ggcgatgtcg gggtcttttt cgagatcgac tcggtgccgc
    25981 ctgatgacga gcgattcgcc tttctttggc gtccgaaaga cggctccgtg gttccgcagc
    26041 ccgcaacttt tcgcctgcgc acgatccgtc ttcgcggaat gctttcgcag gggctgctca
    26101 tgccgctgcg gcagtttccg gagattccat ccggcctgac caccagcgcc gatgtcacgg
    26161 aaatcctcca aatcacaaaa tgggaaacgc aaatcccgct caacgatgaa gtggacggcc
    26221 ccttcctccc cggtgtgccc aaaacggacg agatgcgcgt gcaaagcgtg cccgacgtca
    26281 tcgccgagat cactggaaag ccctacgtca tcaccgtgaa gtgcgacggc acttccgcca
    26341 cctacggcat gaaccgccac accggcgtct tcaccgtttg tggccgaaac tggagcatca
    26401 aacgcggcac caatgcctac tggcatgccg cggagaaata cgacctcgaa gccctgctcg
    26461 ggcgattccc tcaatggatc atccaggccg agcttgtcgg tcccggcatt caaaagaacc
    26521 gcctcggcct caaaggcatc gagctgcgtg tcttcaatgt ctatgaccag cacgttggcc
    26581 gttacctcga tcacgacgag gcgtcagcca tcctctccga tcgcggcctt tccatggtcg
    26641 atgagctgga acgcggcgaa agcttctcgc acgatcaggc ctccctgctc gctcttgccg
    26701 aaggattcta ccccggcacg caaaacgagc gcgaaggcct cgtcatccgt ccacagacgg
    26761 aaacgcacag ccaggtgcta ggtggccgac tttcgttcaa ggccatcagc aatcgcttcc
    26821 tgctcaaagg cggcgattga cccgtgatgc agacactcct gtctgcatgc gcgtgagcaa
    26881 accaatcatc tgaggggcag acaagagtgt ccgcctcact tcaaaccaac cttttcaatc
    26941 aacgggccgt cggtcccgcg aggaaactcg aaaaaccgat gggggcatgg ccccgacagc
    27001 cggtgtgagc cggtcagagg cgaaggtgcc tgaaaacctt cgcgccgtga gtcatcacgg
    27061 acagcccatg caagcccagg cctcccgcga aagcgggcga tcatggctcc caagccgtgg
    27121 tcgtcatggc gatgcaggta tcgtggaaag aacggcggct cagatgcccg gaaacgggga
    27181 acatcagccg ctggagagta acgggtggtg ccgacctcac gatgaaccag cctgcgaacc
    27241 agcatggcca aagggtagtg ccagccattc gagccttgtc ggcaagggtg acggcgcaga
    27301 gcgagaggct ccggccgcag cgatgcggac gcggtcttga gattccgtcg agtaaccggc
    27361 aggtgaaagg cagatggtgt gtggcatttc gcagctcaaa aggcggcgac aaccactgga
    27421 cacacgcatc gtcgcaggtt tgcagctcct cggaagagca tctgactttt aatcagattc
    27481 caaacgcaaa gaggtgtccg gtgtgacgag ccaaatagct caatcctcca gccaggttgg
    27541 caatggaggc acccgagggc cgcaagcccg gaggtgtcgg tgtgaatccg cacataaggg
    27601 gcagctcccc tgaacggccc caaaggtcgg tggtaggtgc agcgccgaga atcttcacac
    27661 gacaagtgaa acgccacact tcaacaaagg cagctatgct gttcgacctc gttcgacggg
    27721 gtggatacga gccgaaccat cggccgcaaa ccgatggata atggccccaa gggagcagca
    27781 cgcgaggtgt agtctcagcc ctcgcccttt ttggcatgga gcgtggagtc aaacatcagg
    27841 ctccacgctc catgccagct catttcatga ccctgtcgtc taactggcca agacatccga
    27901 ctttctatcg gatcactgcg ggttcgaatc ccgccagggt tgccactttt catgctcatg
    27961 tagtccaaca gcagagacac gacgtcgaga gcgtcgccag tgcgggtgca aatcccgcct
    28021 tgagcaccat tttcggttca aagcttagac agcgaagcag ccggctttta accggcatag
    28081 cagggtgcat gtccctgtgg accgaccatt ttccgcccgt tggtgaaaca gcatcacgtc
    28141 tggcttacat ccagaaatcg agggggcaga accttcacgg gcgaccattt ttatctccag
    28201 cggcagccca aaccatgacc gtgccttcac cagcaccatg gcctgcggga gtgccccgcc
    28261 gctggtggcg gcggcatcat cgcgtcgttg aggacggccc ttggcgggcc gtccgttgca
    28321 tttccgcaat cctcaaccca acaacgccaa ccgccatgaa actgaccaaa gccctcaaac
    28381 tcaaaaacca gctcgcaggt gatgtgacga ccctcaagga ccgcctgacg aagcagaact
    28441 cccgcgccgc ctccgtgccg ttcgattatg acagccgcca ggtgctcgcc gatctccgca
    28501 cgaagctgga cgacctcgtg aaggtgaaag cctccattgc ggtggcgaac gccgcccagt
    28561 atgagcgcat cttccgtctc gcggagatca agggtctcgt ggcactgctc aaaagcctcg
    28621 atgtccgcca gggcgtgttc aaggaaggcg gcagctacat gcaggccgcc ttcgaggtgg
    28681 aatacatcgc ccagatcaag aaggccgagg tggaccagct cgtcgccgaa ctcgaggcgg
    28741 agatcatcac gctccaggac gcgctcgacg agttcaacca cacgcactcc gtcgcccagg
    28801 cggcatgaat ttgggtctgc gttcggcttc tgcgcggcca aagcctcaaa ctaggtgtct
    28861 cataaacacc aacgcgtctg ccaaacgtgg aattccgatg tcgatgccga gcgtcaaccc
    28921 gcatcatgca acggatcaag ccgcaaaccg caagactcag gcttcaaacc tcaaccgggc
    28981 ttctgccccg cgccgaaacg ccgcacgact caaactccac gcacgccacc cggcgtgcgt
    29041 ggagacattc attcacaacc attgatagaa aggaggttct ccatgcaaaa caacgaagta
    29101 ttccagcacg tccgcgacgc gctcgctcgt ctcgaaagcg agcggaacgt gcgcgtgctc
    29161 tatgcgtgcg aaagcggcag ccgtgcgtgg ggattcgcct cgcacgacag cgattacgat
    29221 gtccggttcc tctacgttca ttccagcgac tggtatctgt ccgtcgaaga ccgtcgcgat
    29281 gtgatcgagg aaccgctgaa cgatgaactc gacgtcagcg gctgggaact gcgcaaggcg
    29341 ctgcgactgc tccgcaagag caatcctccg ctgctcgaat ggctgaaatc tcccgtcgtg
    29401 tatcagcacg acgcggtctt cacccgcgag tttggcgaac tctcggagcg cttttattcg
    29461 cctcgtcgct gtttcgccca ctacctgcac atggcggtgg gcaactggcg cagctacctc
    29521 gaaggtcgcg agcaggtcag cctgaagaag tatctctacg tcttccggcc aatgctcgcc
    29581 tgccgctgga tcgagcgttc gctcggtcag gtgccaatgc tcttcaatga gctggtcgaa
    29641 ggcgtgctcg aagaacgcga agttcgcgat gcgctcaacg cactcgtggc ccgcaagcaa
    29701 gcgggcgatg aactctccgt cgcaccaccc gacgaagtgc tttcccgctt cgttgtggcg
    29761 gaactgcaac gtctggaggc tctcaacgag cccgaagagg tcgcaggtga cgtgagtgtc
    29821 ctgaacagct tctttcgtcg ctatgcactg aatggcgtct agccgcccga tggcagaatt
    29881 ggcagcttgg aagggccgca aacccttcca agctgtattc ctccaacttc attatcactc
    29941 aatgaataga aatgacgcca tgcctgccgc tctcgctgat tccaaggcgc tcagagcttt
    30001 ggtccatgag gagttggaca agcttcccga tcattgcctg gaggaggccc ggaagaagct
    30061 cctggagctg caactgcgtg agcttgtcga tgaactcgac gcggagatgg acaaagcatg
    30121 ggcttctggc cgcatcaccc aggagagcat tgaagctgcc ttccaagagc atcgccacag
    30181 gctccctacc gatgatgacc gtctgcatgg cgctactggc gtgaggcaag gctcggccac
    30241 aatgccagtg atatagcgaa aagacggcat gtatcccggc tggcctgctt taaatgccgc
    30301 caggagtgaa tggctgcatc gactctgctg accgcgttaa gcgcctcctg cctcgagaac
    30361 accacagaca tgtcataatc agcctcatga cgttcttctt gaagatcgat gaatgtttga
    30421 gccaccagac gaatgtgctg gctgcccgga atgttgaact gcggccagaa tgtctgaagc
    30481 agagagtgaa cttgctgggc attttgtttc acgaactccc tgcacaaaga cttcattttg
    30541 ccatgatcga aagccctccc cgccatttga cgcatgcgct ggtcggcgtg cgccgcgcca
    30601 cagacgacca aagcagcctc ctcaatcaga aagtgaaaca aggcatagta tgaggtcgaa
    30661 atggagcgcc tgatggtcgc ttgcttgggt ctgcgaggct cgcgtgtcgc gagcatccga
    30721 gcctgagaaa gtaaatcggt gcttatgcgg gccatcagcc gatttgcttt gtaatttcgc
    30781 tagctcgctt aacccggaca tagggccact gttcctcgtc cgcactgctc caaacagtgt
    30841 ccctgaccca cgaaaccatc ggctcaactt ttgaccaagc gagttctgaa tctggagttt
    30901 tgtccggaaa gactagataa acaaacaagg cgtcttcacc gtcactatct ttgcctttta
    30961 gcaactgcac atcttgagga cgcgagaatc ccttttttgc aatgagttgc ggctgaattc
    31021 tggccaaaat gtctttcaga ttgaccggcg atgtcctgga taatggcatg gcttaaagtg
    31081 agtcacatat agtaaagcgt caatcgcaag cctgagtcaa ttaatagaac gaacaccgct
    31141 gccgggaccc tcgtgcccat ttccaatctt ccacttctcc attacccgcc ttctccaaat
    31201 ctgaacctcc attcccatga aaccaaacga cctcatccgc ctcggcgtgc ctcagggcgc
    31261 ggcgatctcc tctggcatgg acttcatcgc gaagttcatg gcgcagggtg gtgattcggc
    31321 gcgtctggag gaagaactcg gcgcgatcat cgcgaagccg gagtcgtttc tcggcgatcc
    31381 gctgcgcgag agctttgcgc gtgatcttta cgcgccggcc tacaagcagc gtgatgtgct
    31441 cgcgccttgg gcgcagtggg gttccggtct cgaagcgcag gcggtgcagc agatggcgaa
    31501 cgcgtgcgcg ctgccggttg cggtggcggg cgcgttgatg ccggacgcgc atgtcggcta
    31561 cggactgccg atcggcggtg tgctggccac ggaaggctgc gtgattcctt acgcggtcgg
    31621 cgtggacatc gcgtgtcgca tgcgtctcac ggtttacgac cgcaaggcga gcacgatcgc
    31681 gggtcagaaa gaccgtctgg cgaacatcct cgaagacgaa acgcgtttcg gcatcggctc
    31741 cacattcaag gaccgtcgtg atcacgatgt gatggacgag gactgggatg tctcgccgat
    31801 cacgcggcgt cacaaggaca aggcgtggtc gcagctcgga acgagcggct cggggaacca
    31861 ctttgtcgag ttcggggcct tcaatgtgtc ggcggacgag tccgtggcgc tgaaggagtc
    31921 cggtttcgac atcaaaccgg gcgaatacat cgcgctgctg tcgcacagtg gttcgcgtgg
    31981 tgcgggcgcg caggtgtgcc agcactacag ccgcatcgcg atggaccggc gttacgatct
    32041 gccgaaggag atgaagcacc tcgcgtggct gacctttgag gaggaagcag gccaggaata
    32101 ctgggccgcg atgaacctca tgggccgtta cgcggcggcg aatcatgcgc tcattcacaa
    32161 gcacatcgcg aagaaggtcg gcgcgcatgt catcctcgac atcgagaacc atcacaactt
    32221 cgcctggaag gaaacgcatg tcgtcaacgg cgcggagcgc gaggtcatcg tgcatcgcaa
    32281 aggcgcgacg cctgcgggtg ccggtgtgct cggggtgatt ccgggctcga tggcgacgcc
    32341 aggctatgtg gtgcgcggca aggggaaacc cgagtcgctc ctcagcgcct cccacggcgc
    32401 gggccgcgtg atgagccgca gcaaggcgaa ggagagcttc acctggaacg cggtgaagaa
    32461 gcagctcgcc gccgccggtg tcgaacttct cagcgccggc atcgacgagg tgcccggcgt
    32521 ttacaaggac atccacggtg tcatggccgc gcagacggac ctcgtggatg tgcttggcga
    32581 gtttcgtccg cgcatcgtga agatgtgccc ggagggaccc gcggaggatt gaagggataa
    32641 attgaaagtc gaaatgccaa cctgaaaaag cacagacgac gcggcatgag gagccgtcac
    32701 tccttaccat cgcaggcagg atgccagcgc ctcatgtcgc attccaacaa acttgccact
    32761 tggcaaaccc gggccgccgc atagtttcgg cgtcccagaa cgaacaccgc tgccgggttc
    32821 cccggctgtg atgatcggga ccctcaatcc tcagccatga cttatgagtg acgttctgaa
    32881 taaaacgacg gttttggtgc tgaaccgaaa ctggcaggca gtcggcgtga aaacacccgc
    32941 cgaggctttc ggcatgatgg ctgcgggcaa tgcgacagcc ctccagatca gcggcgatga
    33001 cttcatgagc ccggtgcgct gggaggactg gctgcgcctg ccggtgcgtg atgacgacaa
    33061 cagcataggc actgttcacg gtcctgtgcg cgtgcccacg gtgctggtgc tggcgcgctt
    33121 cgacaaggtg cccaagcgac gcccgaaatt ctgcgccaag gccatctggg agcgcgacgg
    33181 cggcgtgtgc cagtacaccg ggcgcaagct caagcccaac gagggcaaca ttgaccatgt
    33241 ggtgcccgtc tcgcgcggcg gcaagagcac ctgggacaac tgcgtgctgg ccgacaaaaa
    33301 ggtgaacagc aaaaaaggca gcaagctccc cgatgaggcg ggcctgaagc tgctgcgccg
    33361 ccccttcgcc ccgcgcgagg tgccggtgac ccatctcatc cgcaacacct acaaggtgcg
    33421 ggattgggag atgttcctgg cgcatcccgg agtccatgat tcctgaggcg cttctcggac
    33481 cgcagggagg cggagcgccc gcgagatcag gctgatgttc aagaggctgc cgggcgcgct
    33541 gcttgggcgc atgttttttt gcggaaaacg ggacgggcgg ggaggtgccg ttttgaaaac
    33601 cgttgcgccc gcgtcccgct ggtgctccct tcgcgccccc ttgccggtgg cactccagcc
    33661 atgcggccca atctcatccc acatctacct accatgtccg acgccaagtc ctccctcgct
    33721 caaatggaga agatcgtttc tctctgcaag cgccgcggct tcatctatca aagcagcgaa
    33781 atctacggcg gctgcggcgg cgtgtgggac tacggcccgc tcggcgcgga gctgaagcgc
    33841 aacctgcgca ccgcctggtg gaacaccatg acccgcgaac gcgaggacgt gctcggcctc
    33901 gatgccagca tcctcatgaa tcccgccatc tggaaggcca gcggccacgt cgataccttc
    33961 gccgacctca tgcgcgagtg ctcgctgacg aacaagcgag tccgcgccga tcatgtggac
    34021 ccgcaggaag gtgtggtgat gaattatagc ggtgccaagg ccccgaacgg ctggaagctc
    34081 gcccgcgcca tttccgtgct catgaaaaag ggcgagcaca tcgaaagcct gcgcaaacgc
    34141 gtgcgcactc tgattgcgtc caatgcaggt gaggccgcgg gcaaggtcga agagatcgaa
    34201 ctgctcgatg aagccaaggg cgacactttg gagaagacca tcgacttcca tcctgaaacc
    34261 ggtggtgcgc tcggcgaggc acgtccgttc aatctcatgc tcaagaccta cctcggccct
    34321 actgcgaccg aggcggatgt gacttacctg cgtccggaaa ccgcgcaggc gatctttgcg
    34381 cagttcaaga acgtgttcga ttccagccgc gtgaaggtgc cctttggcat cggccagatc
    34441 ggcaaggcct tccgcaacga ggtcacgccg aagaatttca ccttccgcag ccgcgagttc
    34501 gagcagatgg agctcgagtt cttcatcaag cccgacgaag ccgtcgaaat catcagcggc
    34561 gaagtcgcga agtggcatga aggcgccgat ttgagcgaac cgcagccgaa ctggggctgg
    34621 gagatgtggc accgttactg ggtcgatcag cgcacgaagt tctacgaggg catcggcctt
    34681 ggcgatgtgc tcgaatacta ctggcagacg cccgccgacc tcgcgcacta cgcccgcgcc
    34741 tgcgtggaca tcctctgcga attccccttc ggcaccgagg aactggaagg cattgccgcg
    34801 cgtggtgatt tcgatctggc cgcgcatcaa accgccagca ccaagtccca ggaaatcttc
    34861 gacgaagaac tcaaaaccgc cgccgccaag ctcaccgacg agcagaagga agccctcatc
    34921 cagaaacgtc tcgccgccga agccgcgaat accaagggcg agccgatgcc cgaggccgcc
    34981 gtgcgtgcgt ggttcgagcg tctcttcaaa ggcagctacg tcccccacgt catcgaaccc
    35041 tccgccggac tcgaccgcat ggcgctcgct gtcatcgcca aggccttcag cgagaccgaa
    35101 aaagtcgatg agaagggcaa gaaggaagtc atcaccgtcc tgcgcctcca tccgcgtgtc
    35161 gccccgatca aagtcggcgt cttcccgctg ctgaagaaca agcccgagct cgtcgccaag
    35221 gcccgcgagg tctatgcgct gctcaagaag catctaaatt gcttctacga cgaaaccgcc
    35281 agcatcggcc gccgttacgc ccgccaggac gaagtcggca cgcccttcgg cgtcaccatc
    35341 gacttcgaaa cctgcggcga aaaaggcccc gaactcgccg acaccgtcac cctccgccac
    35401 cgcgacagcg gcgagcagga acgcgtgaaa atcagcgagt tgctcgggaa gctgctgccg
    35461 ttggtgagct gattcttggc ttgaagcctc atggctgggc cgttaaaatc cagccatgag
    35521 tactgtcatc gagatcgaaa aggccatcga agcccttccg caggaggagc tgggcaagtt
    35581 gcgcgagtgg tttaacgaac gtgaagccac cctcgtcgcg tcggcgagca cctttgccct
    35641 ttacgacggc gaggagggcg aggcacagca atgggaagac tgaccgcctt gcgaggtgaa
    35701 atctggctcg tggacctcgg catgactcaa aagacccggc ccgtgctcat cttgagtgtg
    35761 gcttacaaag gcgaagagcg tgccttggtc agctacgtcg cccgcaccac cagcctgcgc
    35821 ggcacggagt atgaagtgcc ccatcaagca gccaagttcc tgccgggggc cttcgacgtg
    35881 caatccatcg gcaccgtgcc ggatgtacag ttggtccgcc gcctcacggt ttgcgatgca
    35941 gggacactcg ccaaggtcga agatgccttg aagcgttggc ttgccctacg ctgaatcagc
    36001 gtccagcgcc aaaggcgcgc cccatcccag cctgggccaa cggcccaggt ggtggggacg
    36061 caaaaaacca gatgaagggc tgaaagcccg ccccatgctc cgaccgccat gccgcagtcc
    36121 ctgagcctcg tcatagtcca tctcgtgttc agcacgaagg accgtctccc gcgcttcgac
    36181 gccccgctgc gcccgcattt gcacgcctac ctggccgagg tggcgcggaa cgcgggttgc
    36241 gaggcctacc gcgtcggctg cgtggccgat catgtgcacc tcgccatccg tctctctcgt
    36301 accctgacca tcgccgatct ggtcgaaacc ttgaagacct cgtcctccaa atggctgaag
    36361 acccaatccg ccgccttgag cgacttcgcc tggcagcgcg gctacggttg cttctccgtg
    36421 gctccggcag acctgatggc cgtcacgacc tacatcgaca accaggagga acatcaccgc
    36481 acccgcacgt ttcaggagga gtatcgggcg ttcttgaaca aatacggcgt gacctatgac
    36541 gaggcctacg tgtgggattg aggcgcagcg agcatggatc gggctttcag ccctcggcgt
    36601 cgggtattgc cttgttcctg ggccgctggc ccaggctgga atgaaccgcg cctttggcgc
    36661 tcaagccccc cagacaacat caacaagcga gacaccacca tcaccttctg ccgccgtaat
    36721 cccgtctccg ttcccagcgc caaaggcgcg accgatacca gcctgggcca acggcccagg
    36781 gggccgggaa aggccccgat atcaggggaa gggctgaaag cccgccccat gcatgcaccg
    36841 cgttggtttg gcgatcaggg cgtggggaag agacgtgtgg tgtgcatgga tcgggctttc
    36901 agccctcgac tgggggtgtt cgtggttttg ggcacgcatt cctgggccgt tggcccaggc
    36961 tgggatagaa cgcgcctttg gcgctcaaga tgcctctcat agagatggcc gtggacatgt
    37021 gcacgaaaag ttgggggttt caggccaaca gggccgcgat ctcagagtga ccacgctcag
    37081 aagccaagtc ctttgcagtt cgttctttgt tatcacgcaa ccgcgaatct gcaccgcgct
    37141 gtaacagttc gcgaactgca tccatgcagc caaaccaagc ggcgttcatg agtggcgtgg
    37201 ttcctccttc atctctggcg ttgatatccg cccctgcatc aatcaacacg cgaacgcagg
    37261 ccgtctggct ggaatcgccg gactgggcta cagcccaatg cagtgcacta catccacgtt
    37321 tgtctgtcgt tctggcacgt cctccacgcg acagtagaag tcggagcaca tcgacactcg
    37381 cacctcgggc ggcccccatc agagcggtat atccgacctg attcccgaca tcaggatcga
    37441 tgccgttatc gagaaacgtt cgaacggctt cgaagtcgcc ccgatatgcc gcccggatag
    37501 gatcgtccag atgcgcttca aaaggaaatc gccccttggc agtgagagcg tctcgccatc
    37561 gttgcatgat ctcgcacaaa ggcggaatcg aatcgtcgat gtattcatcc accagttgct
    37621 gctcttcatg ggtcagcttg agaacactgc caatacactc gcacgatgcc agttgcccgc
    37681 cacagaatgg gcagcgttct ttggtgcaga aaagatcgtg gagttctcca cttcgcacgc
    37741 cgcagtcggc gcatgtttca accatgggca ccctttgttc aggctctttg gtctttgatc
    37801 ctagtatcag tcttttgagg gcgctaaaca tgctgggaaa cgttgttatg ggctagagtc
    37861 gagaaccggc tggggtgtac cgtccggcta acatcgcacc aaaaaatacc cgaagtccat
    37921 ctcccagccg cgttccgtct tcgtcgcggg gatggcgggg gcgaggtggt agaggaggcg
    37981 gcggccgtcc tgcgggttct cggcggtgac gacgaggccc gcgccgcgca gggcgacgag
    38041 gtgtttcagc gtggcgtcga gccgcttgcc gacgttcccg gccagaaccg tcgcggtgcg
    38101 cccctggcca tccgcgagga tctgaagaag gcgacggcgg gtgggatcgc ccagcgcggc
    38161 atagacggta tccgccggga tgacttgcgg cggcggcgcc gggattgtgg gaggggcggc
    38221 gggcgtgctc atgcctcatt ttgcccaatc cgccgactcc tgacaagcgt gcagactccg
    38281 taatcggagc ggggaagact gccaataagc gggaggactc cgcaatcggg catggggaga
    38341 gtgtcaacaa gcgggaggac tccgcaatcg ggcatgggga gactgtcaac acgcgggagg
    38401 actccgcaat cgggcatggg gagactgtca acacgcggga cgactccgta atcgggcatg
    38461 gggagagtgc caacaagcgg gacggctccg ggatttcgct gcgagagtgt ggctggtgtg
    38521 ctgctgggga ggcgtggcgg gtggtgcggc tggatggcgg tggatggggt attcgagcag
    38581 ggttgggatg ccagtcttct gacggggaag ggaaattcgg gcgcggcttt ttgctcgacc
    38641 tggtgtttga gtccggcgca ttttatgact gtgaaatttc cctcgagttt tcgtgccagg
    38701 ctgattgtga cgatttttcc tgtcgtggcg gggattgcgc tgggggcgct gctgctggcg
    38761 gagtggaagt ttgcggcgac gtatcagcgg ctttttgagg agcagttcga gagccagatc
    38821 gcggcggtgg cggaggcgaa ggcgcggcgc tttgaggcgc tgtcttcgga gctggagagg
    38881 ctggcgctgg ctccggcgct ggtggcggcg gtgaaggggg gggatttttc cggggcgatg
    38941 caggagctgc gcccggtgct ggaggcgctg gcgacgagcc ggctgcaaag cgagtttccc
    39001 ggcgcgccgt tgccgcgcgc gtcggg
//
LOCUS       DAOMYJ010000022        38457 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Prosthecobacter sp. isolate AMR_MDS_5629
            NODE_401_length_38457_cov_24.730301, whole genome shotgun sequence.
ACCESSION   DAOMYJ010000022 DAOMYJ010000000
VERSION     DAOMYJ010000022.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867850
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prosthecobacter sp. (wastewater metagenome)
  ORGANISM  Prosthecobacter sp.
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Verrucomicrobiaceae; Prosthecobacter.
REFERENCE   1  (bases 1 to 38457)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 38457)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.30
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:34:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,742
            CDSs (total)                      :: 4,684
            Genes (coding)                    :: 4,676
            CDSs (with protein)               :: 4,676
            Genes (RNA)                       :: 58
            tRNAs                             :: 55
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..38457
                     /organism="Prosthecobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_401_length_38457_cov_24.730301"
                     /isolate="AMR_MDS_5629"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1965333"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..778)
                     /locus_tag="PLP58_04040"
     CDS             complement(<1..778)
                     /locus_tag="PLP58_04040"
                     /inference="COORDINATES: protein motif:HMM:NF013630.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HRJ07713.1"
                     /translation="MAKAQLKLLLYLIVLGVTLGCMAVAWYIYIKVLAPERRLRQEMS
                     EIKRSDMPRIDPGMKRFEAAADMLNEGRVPEAREAMFRLIQQFPDSASCGEAKRIIGE
                     INMDELFSTEPIPGKRDYIVQPGDSLALIASRQGTTTDMLIRLNGLMSTTLHPGDHLT
                     LMPLEFNVVVDASEKTVTLRRVLGGREFFFKEYKALDMRLPAGVKPPAEMEVRGKSAV
                     LDGRSLLPADARYMEADKWLPGSRSGVVLRTPPPVRAIPVT"
     gene            complement(804..1334)
                     /locus_tag="PLP58_04045"
     CDS             complement(804..1334)
                     /locus_tag="PLP58_04045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009961681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ07714.1"
                     /translation="MHTEYDIQYALEMTRVLHEPDRRIDTFGSTQFEFQLVSELMDQV
                     NATRVRQGVLTAERPLILRPDPSAMADFDFDGFGPQGAAFGNFLKENLHKLAVLKYGF
                     KFRMNDMNEEVVHESMDDVCGKLLEDIRATGNPMRAVIAGVDDTWEICLLKFTVEMIE
                     KSQKINLFDFKRRGLL"
     gene            1575..2288
                     /locus_tag="PLP58_04050"
     CDS             1575..2288
                     /locus_tag="PLP58_04050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0009254 - peptidoglycan turnover [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3D domain-containing protein"
                     /protein_id="HRJ07715.1"
                     /translation="MKVTLLLCWVSSISMALSVLSSCALPQGGFLKKGGVTAAKGSPL
                     QPGVRYEVRKALQAGVPFRPQEMKRYFFVIETPRPPMMAAANAKAGQMTVRTTAYCHD
                     ESDHIVYGRKNALGTDLKFGSVRSAAADWSRYPVGTRFRIAGQPDVVYEVDDYGSALV
                     GTGTIDLYKPTQGMMNQWGVRHVDIEVLQWGSYERSMEIMRDRVRWPHVRRMVSDIES
                     NVMQATMKSLKPQPLAAAL"
     gene            2368..3573
                     /locus_tag="PLP58_04055"
     CDS             2368..3573
                     /locus_tag="PLP58_04055"
                     /inference="COORDINATES: protein motif:HMM:NF012958.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase-fold protein"
                     /protein_id="HRJ07716.1"
                     /translation="MRVLILLIAFLLPCHAQNSAQSGPLKKLQSVLQAPPESASATAL
                     DGQVTRLFGRQNLMQGKPGAKVEGTTVAWAFMDMQPARVVRGDGELIGEMTRLGEDGL
                     QVLVREMPNFHEFQYRIEVDGSPRVAGMVRVEHYEYGADSERRAGVPEGALEKFAWNG
                     SKVFPGTERDVTVYIPRQYRSGEEACLMVWQDGTRHADPHGPMRAGVVFDNLIHKKEM
                     PVTVGVFVDPGRKPGQKAGDKAANRGFEYDSLGDAYVRFLLTEILPEVEKRFQVKFRP
                     GPEARAIAGGSSGCICAFTAAWERPDQFHKVLGWVGTFVDIRGGNAYPYLLRVTERKP
                     IRVYLLDGVNDLDNQFGNWPLANRMMEASLKFMGYDHRVDWNECFHGSKGMGPSLPDA
                     LRWLWRDVR"
     gene            complement(3658..4782)
                     /locus_tag="PLP58_04060"
     CDS             complement(3658..4782)
                     /locus_tag="PLP58_04060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009964321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate synthase"
                     /protein_id="HRJ07717.1"
                     /translation="MAVVSKGLEGIVAAETRLGEVNGAEGILYYCGYDINELAGKVSY
                     EEVVHLLFYNKLPNRAELDRITTALRAERELPQGVIDFLRHAPKKARPIDVMRTAVSM
                     LGCYDMARHDVDMSDNLATAIRLVSQIGVIAAYFHRARQGKELPPVRKDLSEAAHFLY
                     LMNGEAPSKEAEKTLDVAYVLHAEHGFNASTFTARVVASTLSDMYSAISAAIGALKGP
                     LHGGANEGVIHMLEEIGSPDKVDAWVEDALAQKKKIMGIGHRVYKVLDPRAPHLREMA
                     IQLTAQLGEPKWIQMSERIAEIMRERKNLNANVDFYSATVYYSLDIPTDLFTPIFAIA
                     RMSGWTAHVLEQWSENRLFRPLSSYVGRPYGQKVAPIDER"
     gene            complement(4918..6000)
                     /locus_tag="PLP58_04065"
     CDS             complement(4918..6000)
                     /locus_tag="PLP58_04065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009963471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ07718.1"
                     /translation="MKTITLLICAFALALQTHAAEWTIETFAGTGEKGFSGDGGPATQ
                     ARMDNPFGVIRGPDGAIWYCEYTGQRIRKITPDGVIHSVAGSGQKGYSGDGGPALEAA
                     FNLPHEIRFDAKGDLYIVDMANHAVRKVDMRTGVISTIAGTGKPGHSGDGGPAVRAEM
                     NKPHSIQFGPDGHLYICDIGNHVIRRVDLATGIITRYAGTGEAGPTPDGSPIEGTPLK
                     GPRSIDFDKEGNLWLCTREGNQVFKFDVKAGKILHIAGTGKNGFTGNGGPARKATLKG
                     PKGIAIDAEGNAWLADTESHSVRMVEAKTGRLLLMAGTGEKGDGPDGDPLQCKMARLH
                     GIYVDADGSVFIGDSEAHRVRVMRRK"
     gene            6343..7926
                     /locus_tag="PLP58_04070"
     CDS             6343..7926
                     /locus_tag="PLP58_04070"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: protein motif:HMM:NF010696.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HRJ07719.1"
                     /translation="MSTTNSLWTRFQKHFLRHPQTGFSMDVSRMNFDEALFDQMEPAI
                     TRALTAMRELEAGAIANPDESRMVGHYWLRNPGLAPNEDIKAEIIRTLDATLAFAADV
                     HAGKILAANGQKFTRVLVAGIGGSALGPQLVADALTPANPPLAIDFFDNTDPDGMDRV
                     TARIGAEIATTLTLVISKSGGTKETRNGMLEAAAAYAARGVDFARHAVAITGDGSELD
                     KHAISQGWLARFPMWDWVGGRTSVMSAVGTLAAALQGVDVRQFLAGAAAMDAETRKRP
                     ARENAAMLLALMWHHAGGGRGAKDMVVLPYKDRLVLFSKYLQQLVMESLGKELDLDGR
                     VVNQGIAVYGNKGSTDQHAYVQQLRDGVNNFFAVFIQVGRARDTAGMEVEPGYTSGDY
                     LQGFLRGTRKALAQSGRESITLGISELNAFSLGMLVALFERAVGFYATLVNVNAYHQP
                     GVEAGKKAATEFLAQLGQVRAALPASGAGVTAEEIAGSLGIDPEDAWHMLTHLAANGA
                     AKITIAGDTPGGDRFTAAG"
     gene            complement(7992..10121)
                     /gene="ppk1"
                     /locus_tag="PLP58_04075"
     CDS             complement(7992..10121)
                     /gene="ppk1"
                     /locus_tag="PLP58_04075"
                     /EC_number="2.7.4.1"
                     /inference="COORDINATES: protein motif:HMM:NF003921.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009358 - polyphosphate kinase complex
                     [Evidence IEA];
                     GO_function: GO:0008976 - polyphosphate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006799 - polyphosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase 1"
                     /protein_id="HRJ07720.1"
                     /translation="MPTPTPPVPVTEEHFFNRELSWLAFNERVLEEAARRDLPVLERL
                     KFLAITASNLDEFFMVRVGALQLLREQGMRHKDAAGLTPTQQWERIHERATAFVARQY
                     EILEGQLRPLLWQGNIRRLRVSDLTPAQRAHLEDHFNEHIFPVLSPIALDEDAPRITV
                     PALQIALLCAVLSEQDGRASRRHVLFTLPSNLPRHVTVPDAETAAHCYVNLEDLIELF
                     LQHYFPSEKVTDCARFRISRNSDIAVDDESAFDLASEMEDVLQARLLSPAIRIEIEQG
                     APRDLVKIIRDLCGARTAQVFTIPGELDLRACFQIASLSGFDELKVETWETHPSHQIE
                     PGQSIFEAVKAGDILLHHPFESFDPVLQLIEEAAADENVIAIKQILYRTAKNSRIISA
                     LIRAAQAGKHVTVLVELKARFDEARNLERAEDLLNAGAQIIYGVRGLKTHAKICLVMR
                     REAGHLVRYTHFGTGNYNESTARLYTDISLLTARPGFGADASAFFNTVTGRSRFVHFE
                     RISMAPFGLRERLLSMIRSETERARQGESAAIMLKMNALEDRRMIEALYEASQAGVKV
                     RLNVRGICCLRPGVKGVSDNITVVSIIDRYLEHARIYWFRQGGRPVVFISSADFMNRN
                     LSKRVELLVPVEDKDAKKRLTHILETHFADTSRGRHLRADGAWIQPAVPAARAVRSQL
                     SFTQEAARRARARNQAPDLLVPHVPKD"
     gene            complement(10170..11690)
                     /gene="guaA"
                     /locus_tag="PLP58_04080"
     CDS             complement(10170..11690)
                     /gene="guaA"
                     /locus_tag="PLP58_04080"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018969275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003922 - GMP synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006177 - GMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HRJ07721.1"
                     /translation="MTENEVAVLDYGSQYSQLIVRRVRELGFASHLYPPAELPNLQNP
                     GAIILSGGPRSTSEKDAPDVDYDKLMSFGVPVLGVCYGMQLLNIKHGGTVKPGVTREY
                     GPAKLVVTDHEDLFSSISHESQVWMSHSDTCANLASTTKVIATNEDAVPVALKWSDRC
                     WGIQFHPEVTHSHEGTAILKNFLTKSGAQLAKFDINAFKAQMLERIKAEVGNREVICG
                     VSGGVDSTVLAVLLAKAGVKVRCIFIDTGMMRLNEAAEVKTLFDEVGVPIEQIDASAI
                     FLGALKGVTDPEQKRRIIGTLFVEEFWKVADNVELLAQGTLYPDVIESATSGSIASKI
                     KTHHNRVDKIMELKEQGKVLEPLAELFKDEVRALGASLGIPHRALWRHPFPGPGLAVR
                     IPGEITPERVAITQQADAIFINELRRSGWYEQTWQAYAALLPVKTVGVKGDERSYEQA
                     ISLRAVISEDAMTAEWVRLPYEVLSSTSNKILNSVKGVNRVLYDISTKPPASIEWE"
     gene            complement(11727..13187)
                     /gene="guaB"
                     /locus_tag="PLP58_04085"
     CDS             complement(11727..13187)
                     /gene="guaB"
                     /locus_tag="PLP58_04085"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018969276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003938 - IMP dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HRJ07722.1"
                     /translation="MGDIPSLALSFDDVLLLPGLSEVLPGEVEIGTPFGGSIHLRIPV
                     LSSAMDTVTEAELAIALAREGGMGVIHRNCPVTHQAEQVARVKRSENTVIQNPHTVSP
                     GTTLGELQRLMHIKGVSGFPVVDPEGRLVGMVTSRDMWYIEDESTPVSAMMTPLGKLA
                     TGTADTSYEEALKILYTHRIEKLPLINADGKLAGLITKQDVVKRQTFTHAAKDANGQL
                     RVGAAVGVGEDCVERGAALAAAGADALFIDAATGHTTRVAEVVRRLREHLGGSIPVVA
                     GNVVTADGALHLMEAGASAVKVGVGPGSICTTRVISGVGMAQFSAITEVAAVCRPRGV
                     SVIADGGIRYSGDIVKAIAGGADCVMLGSLLAGTAESPGNMVKWQGRTFKEYRGMGSL
                     KAMRKGAGDRYGQNSSGKLVPEGVEARVPFKGPLSDVVFQLMGGLRSGMGYVGANNLD
                     ELRAKARFVRITAGGLKESHPHDVVITEEPVNYEPS"
     gene            complement(13244..13936)
                     /gene="vccD"
                     /locus_tag="PLP58_04090"
     CDS             complement(13244..13936)
                     /gene="vccD"
                     /locus_tag="PLP58_04090"
                     /inference="COORDINATES: protein motif:HMM:TIGR02596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Verru_Chthon cassette protein D"
                     /protein_id="HRJ07723.1"
                     /translation="MTPLRRNKAAAFTLIEMITVVGIIALLVALVTPTLLDVIRSTRL
                     SSSGDSLINRLSLAQQSAISMGSEVEMRFYSYINSSSDRPGDDLFYAYQVVHSPQSGV
                     ERSLSEAYYLESGIILSRTQQLSPLLQTTNRQRPDDAGNFLFRPPGSTEPENVSYAAM
                     RFFPDGSMKMLKGSSSTDEAQAVALDYTVPPYDESFLTIVESKDAGSAQPRNYFCVQI
                     DSYTGKTRVYRP"
     gene            complement(13962..15101)
                     /gene="vccC"
                     /locus_tag="PLP58_04095"
     CDS             complement(13962..15101)
                     /gene="vccC"
                     /locus_tag="PLP58_04095"
                     /inference="COORDINATES: protein motif:HMM:TIGR02599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Verru_Chthon cassette protein C"
                     /protein_id="HRJ07724.1"
                     /translation="MKSSPNPPADRILRVFSGAFTLVEILVSLAVLAILLLISAEVIG
                     TVQTTWTATNARVNQFREARTAFDIITRNISQATLNTYVDYDTNYLNSADPVAATTQA
                     PTRYQRKTELRFVCGPASTLIPTGGASVLPGSAVFFQAPLGVTHDPQFAGLDRLLCGR
                     GYYVQYSSDEFFRPGFITSSTLKYRYRLMEFSPPSERSIIYANQRNAWMQDAGRSLTD
                     SAGRSGGGGNETSINRGMNRPVADNIVALVISPMLDPGSAGGGASSTVIAENFAYDSD
                     EVRLANSAGMGQQGTQYLLPPLVRVVLVAIDERSAERLVQTNGGGDGQSPPFGTQIHD
                     IINGSPASQLNQSLEEVARVLREEFRVNYRIFSTTVALRGAKWST"
     gene            complement(15126..20378)
                     /gene="vccA"
                     /locus_tag="PLP58_04100"
     CDS             complement(15126..20378)
                     /gene="vccA"
                     /locus_tag="PLP58_04100"
                     /inference="COORDINATES: protein motif:HMM:TIGR02600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Verru_Chthon cassette protein A"
                     /protein_id="HRJ07725.1"
                     /translation="MKQLRSILADARRHQGLALIIVLSMLALATIVILAFLSVADTEH
                     KATITYSSSQTARRLADTAVNMVVSQIRAGSERETAAAPVIHATQPGAVRKYSQTGIF
                     IAGYKLFSDKDMIFRSPPASASNPNEFEQQFVANSEPPANWNRDVNIARFVDINEPVI
                     KGQLDASGNSTTTLTIFPVLDPRAAQDIDPNPGDEFPVEGFSYNTATALRNQDLSGAS
                     DINPEQRAIVLPGAAGGSVDSLRLAMPVQWLYILRDGAVGYLDETLRFQVLAGSSGAL
                     GSGESSSPQDQYGVPSETNPIVGRVAFWTDDESCKVNINTASEPTYTGQPIYYHERDQ
                     SWADFPPARSEYQRFPGHPATVALSSVLYPNPTLADNRSLDTYGPRGRITGTALSNAL
                     AVKERLYDLIPRIHTGGSLSGTRSFEQDAYRTLSGDSTNATAVAIQQAAGERLYASVD
                     ELLFSQRDSDRRRVLNNAVAGSTTLFNRQSLERSSAFLTAHSRASEVNMFGLPRVAMW
                     PVSERQDRRTGFDNLIEFCTRLGSAGNNYIFQRTFARTIPPSSSGASYDINIQRNQRL
                     MAMLDALMNKSFPAAVDGAGGAVARSYVQKYGQDNTRQLLVQIFDYIRSTNLYDSYLI
                     PQNRNNWPTFVTSGTASTVDWTQTYRERDRLVAQTRTYTDGVARNPNSTRDGNRSQAR
                     INDPFEDRFLPGHGQVTPAEWSVGGRTYRGFGRFVSISEIGLQFICTADGQPDMYSWR
                     IPMKSTEGGAAATDYQIPVIADEDLVNYVDYFASSPDVPNVSGGRTALKLDENVAFQN
                     TYTIRDLPLGNMAMDEVAITNLDGVRWAGRGDRSIKDRYYSNFPPLNTYASGGLYNTT
                     NLPPNGPGTKNFTRQPARHPGYRAENWNHSLEEDTPLKVDEKRVQALLHLEFFCPSVG
                     YGEIIPDMTFVISANDISNIEVDGKAIFSTTQNVVIRTERPLFETDGTPEVGGFASFR
                     KISLGRRVKGMGRVPDDTGYDTNATSNVHSGVLNMDLVSSFFTVNRNRALQFSSPPIT
                     VNIYDTHDWQSAAPVQVVRFRLSAGQAPTPDLVCAGSYQVNFIRPDGTEYNHPLLQAP
                     RWWGFHRDGVLGRSLGIAVDSEKGRFNRDFAGQRVDTETRAHNPNAIDSGSGIATANA
                     QSVPGARALIYARDSGNYVGVRLLDPGFIADRIKYGPRPGDPQSSWDRPWHFGSDVVR
                     AMQPAHGDARILFVKRVVEPADWTPHRFWNDANEYMAHNFSSYTAGGEPGFDRGTSSF
                     SNLVDNTVRALPPDVTVSAARSPDAPHGRSTLAPTSRAAHAFVQRYYDFDDSDPGGRV
                     GSFINKPDEGNYAVGDFWISGWTPGQPKKWRATYFRSNGQGSRVAAGQGSYFTPNRMI
                     SSPVMFGSLPTRMWDNNGEGAWTNALFRPHVQLPNGVTGGVTSDATHPGETTPPDHYL
                     LDLFWMPVVEPYAISESLSTAGKINMNYQMMPFTHIRRATAVHAVMKGEVFAALPNTD
                     YELSKDVRTGWGTNGSTAPVMRNESLPLTATGAGANRNAYWHRTIAIDRFKPTSGADQ
                     RWWQLAIGQRVQGTLRQFEERFNFGLSQNNSTLPEGMRGGLFRTASQICELHLIPNPA
                     PGGAAVNVSPGDVTDVNARDNAMGRFWAEHCSTGDNTRERPYSNLYARLTTRSNTFRV
                     HVRAQSIRKSLRSVDPAQFDPDRDVMTGEFRGSFLVERYIDQGDLQRAGNAVDYANAS
                     DPFSLRPLEDYYRFRVIESKRFAP"
     gene            complement(20525..37375)
                     /locus_tag="PLP58_04105"
     CDS             complement(20525..37375)
                     /locus_tag="PLP58_04105"
                     /inference="COORDINATES: protein motif:HMM:NF025295.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="immunoglobulin domain-containing protein"
                     /protein_id="HRJ07726.1"
                     /translation="MKRIQPFSRTLAARLLTLFLWTVLIGGAAAQSFRRDGSWLSNAN
                     GTLANPAAFYRTIGSTATATAARGTGGTAQVVVSITVTNAGSGYVTPPLVTITGGAGS
                     GATATANLNASGSVASITVNTGGSGYTANPTVTIAPPPSPGGVGSTVTFNTDITANRI
                     LTLDANRTYGRMILGDLSSTQVYTFNSGAGGGFIFDNGSFSGGNAYFNKFQGGADVIN
                     TSVTLQSDLVARIISNRVTLSGTISGPGSLTSVGNGTLALTGNNTGNNVNLWLWNRNG
                     TGTGAQVELGAATGNATGGDIIIGNTTRGTNGHAVLQLLQGRTNLDQISDSASLIFDS
                     ISGVGRNAYFKLMGGNETVGRILDVGDRAVIENREGEAVGTAAVLTIAGSLTSRVNGF
                     LRDNTGNNLQQADSNGAVSGGARLGITKQGTGNLTLAGGNIIYTGDTLVSGGSLTLFN
                     TTNFRSNITNNSQLNFDVTGTVNMRKSFPAIPGSNPTKDYPDQYLSISGSGSVNKTGA
                     GTLGIFGTQNIGGSLTMNNGTLNLNASAAGISIGGDLISVGDAALNRNINLRGNVSIG
                     GDLEFSGRFNNTGSTMNISGAVLGLGDQELGTWVDTAVNVAGSIHIKQANLVLESDFS
                     IGKTSTGTTSNSNIVTITESSNMVVGMRVTGGGVPGGTTVTAIDAVNRRVTLSNNASI
                     SNATALTFSFSNNTDGRITGTPADITISGRSLAIHAGASGSALILSNTNLSNNTNRLP
                     DSTPIISNGGVFEFINDASAASFSETAGTLVLNGGSLQVIGYRAANGRTSTLTFAGLQ
                     RNPGGVVDFEAKQLVGGVTTVENNTLGTDTRNRIMFTAAPALANGIIGGWAYSNNEWV
                     KYGANGVTQLLAADYNNSNQDVGWIAANNVKMSVAQTLTARRTINSLNIQPDLEAAAQ
                     NRALTLNQQRLDIVTGGLLSNHGDHTINSQALGFVTAGTEALGDRELITIVGTTGNAA
                     AARTLTFNAAIRDYTISRNVTVTNNSTTVNIVGAGQTTTGLAVGMAFIGAGIPDGTVI
                     TVINTSTQFVVSQPISPGFTNQARDFGDPRSLSLVKAGPGFLRLNNLGNTYSGPTVVS
                     NGTLMVGGIGALGTIPSVLREDHFRVEGGSAIVFGRRTDITHPNPAPDNVFDFTDGNR
                     GFSIGPAGGRFEVGFANPNNAPTTGAGEPIVHVNITNPLHAEGPLELAVRANANNGQS
                     NSITIGTPGGSNSFLSGIKTEGSFEGVINIHGNNTIGGLLMEGSTFNLTGDNDFTENI
                     RVLGGTLNITGANTFKGGQDFPSVTMGSGILRLLNPAALGTNGIKVALGNNGQVQLEG
                     INHRFTQLSGNALSRVVNASASSATLTLDLEQNQTFAGQFDNGGGGGLLNLTKSGPGR
                     LTLTGTQSGFSGVVRINDGAIDVRNMPFSGVVSSLGTGGNGAASRLILNGGALSFSPT
                     VQQRTDRSFTLGAGPNGGTLVANGANRAASVTMGVNFILFDELVASDTIGFEGNGARL
                     LTLSGVNTGLNTLALQLGDKSAMEPTGLQKIGSGWWLLGKAADYSGQTSVLEGVLRLE
                     SNYAAGTSSVATTASFAADTFTGNLLNGTELTFPQFNQTTLPGGITGGQRYYVVEANS
                     VAGTFKVSTTPGGQAVNLLSDGANVTYVPNISSVAATTANPDTDRFTGNLPNGTPVMF
                     GTRLVFGATAIDPPLLPVGISENITYFVVNAASGVSGPNFQVSTTPGGAPVNFTTTGN
                     HVYYSASPAGNTSEGIYVMGGRLDLGNVDYTAPETIIFNGGGLGVPIRAQAAWAGNVD
                     TQANSSWTIGLDASLTLTGNILGNRTITQLGEGTVVLKGERLNPTVATANPAVRTDVM
                     DHSRSSYTLQAGTLVLDYSSNNNSKLTDNSTFVMGGGRRGGILRLVGGTHEEIVSTLS
                     LQAGASRVFRDSGASVVRFNTISRQTGSSIYFDGPGLAKVDMPNINGILGGWAVVRDA
                     RVQAFLVIPGTVSRTFSVDETTGMLKVPDVEPVHYLAAGVPVRFTTTGQLPDGLAADT
                     TYYVTEATSRSFRVSATPFGPAVQTVTFGTGTHTVETFGAVKRPGPASLIFQADPNPN
                     PAETSPTGFSGSKGNGTIRVRIAHNGGSNGAISVGRGGSGTVESPWRITITTTAANNS
                     ADAVVAALAGNANALGIVKVFSSAPDASADTNVYYAAPGELLAHGVDDNGGQTYSWAR
                     NATATATSFNDGPVDGFLDYSTDWGLNFNTDVLGGGAPIPVNDGASTYTLRFATGLPA
                     TLDLLAGASGQLLQTGAVLVSPTVGANDSIISGGGTLMAGNEGALQNFVFHQYNDLGD
                     LVVNVPLTQRQSFNRTGRLAAPSVAASTQNRIITGLIGVSDNSDTSQLTVGMTVTGTG
                     MPGGTTIAQIVDNRTVIVSADITTGDNSRNVYTFGGSIQMRGSIRQFAQNRITGVVDA
                     QGNLATHDLYIGMPISGPGIPANYRVQTIVNESDITIGTLNGNGITVDTANHVYTGIV
                     SQLTFTPSVGVEKLGDGTLVLNADSNYSGITFIGNGTLRAQQLTDGGVPGSLGASTNA
                     AANLTFNGGTLQYVGMTSSSNRNFTLSELAEINIGHERTTSVFTGNISGADIFAKSGP
                     GTLEMRGNAGLAEIRLLEGRLVVQGVDTNLAPNAFAQNNFGQTGIGELRLAGGSLELR
                     GAREGNMTLTLGGQMYVDAGASEVRAVGVSGFNPNSLISGAISRTTQISLMGAEEITS
                     VLRNVGGTVLFVEEPQVGGSVANILLNIPETDRARVMPWAVYQDATNIAQPGVNNFAT
                     VALSNGAVISADAASLYDLGSFFMNADNWGTQEGSATIDASEGGRIEISLDNGVESTA
                     GSNVLVVTDLLQVEFGNLIPDMSVFGPGIPSNTQVVSLLTNPLRVVLSKNATSSNFNA
                     TYTFIRDRAYFGTLTADRALNTLRYFTPVDSTITVPAGRTLQLLSGAILAAANVRGGE
                     KAIVGGGNISGAAGAVDGGDLVIHNHNPVAAFTIGANVVDNVLSMVNSGTTSAGSNIL
                     IVSPLGKTALVRLKPGMIISGPGIPDGTTVLSTDLNFDLVYMSANAVSAQSNKTYTFL
                     DVTSFVQSGTGTTILSGVNTYTGRTFVHGGVLRLNSANAMPGGIGNTGGTGGIVVKGG
                     VIGLGHGDFTRDIGTGAGQIDFQGAGGFAAYGSDRTVNIGGQAVPEALRFGNNGFVPD
                     GSSFVLGAHDATHKTIVLNTLDLGSFSQAVRVNDGPAEVEGELAGGFAGLGRLIKFGD
                     GTLRLSGSSSHSGGIEIAAGRLIAADVEDVFGVGTGTGTTVRMGVNSTNTRKDAALEL
                     VVEGGTIEKNLRVGLVNSRGADWLERGSVEESGDLVGTHASTAVVNGHPAIAYYDSTN
                     GDLKYVRAADPRGTSWLPPVTVASRGDVGQFPSLAVVNGNPAISYYDATNKLLMFVRS
                     TDISGVFWGGQVIVDSAPINAIGVQSDGKAVIGGTFAELDGVEKTRLARVNTDGSLDN
                     TFAATANGEVLGIVIQSDDKILVVGSFTEINDVARNRIARLNADGSLDTGFNPNANGV
                     VRGLLIEPDGRIVVWGSFTSIVSTGRNRVARLNSNGTIDTGFNPNANGEVRSVARQAD
                     GNLILGGAFTTIGGTTRNRIARVTTAGALDAGYNPNYNNTVSCILILPNGKALTGGVF
                     TTIDGAGRRRERLARLNTDGTLDQSFSVIANAEVRDLVLQADGKVLAVGAFTTFGDHA
                     RRSLARLHSEGSPESLVDPVDSAFNVDANEEVRAVVQIASGKLLAGGEFSNIGGSTCH
                     FLARLELADGGADAAFDRQTVDRGCYTSLAVVRQQANNGLEAPAIAYYDTLGTRLRYS
                     RALDANGASWAVSLVLDSSANVGAGTSLKMVNIGGDLITKNTTQNTVTITGEAANGTP
                     AVAYYDVTNGNLKYVLANNPAGSDWSAPVVVESANDVGSHLSLNVVDGFPAIAYYDAT
                     AKSLRYVRATNAGGVTNNLRDAQGNILTRAINALVFDPAWGAPGVVDSGGDVGMYPSL
                     EIITDGTSAQGRPAIAYYDAAPGTKNLKYVRALNVTGSFPWGTPQTVASEGDVGMSAG
                     LVLTDGVPGVAYHDATEGDVKFLHLADSTGYSRIVLGDGTLWQGGMNLQGRAFFDVEA
                     AALATISGLVQGDAGITLSAQGTLLLTNAGNNFGDSLPGPGPDVNSAVIVRAGTLAVG
                     ANGAIGQGTVEMGDATPQIMTVERATTFASLAALKGRFDADHNGIFDNAGGPGAFVEV
                     DITIDGRTYTEDDQGTLILVKDERENPQWNGVYQIIFNGDLQPDGTMNLARVAQMDSV
                     AELAYGTQVRVLNGTHAGQAFFIASKVLELNVSAVQWIRDYADSNAALLASTGGLTIS
                     NSIDVNAGNGSGLAVLGGMADFTSGNSTFSGAVLLQNRLPGVRETKTVGLQSFTNTGY
                     GITFSGLFSESNGGNGASQDSMSLSKSGAGTASLTNANTFFGGVTVQQGTLLVLNTTG
                     SATGSGAVAVNAGAVLGGTGFIGGAVTLTGSVGNKAILRPGDPTMVSAVETLTINAPL
                     TVGPESVVEFAIGAANITRLVANSVTVTGTGQFLVQLLDGFIPAVDTVFDLVDGTINL
                     PGGANARDHLVLPGHVSWDTSQFLSQGTIRSTGLTTPVQINTHPVITVPAGGSPVNPG
                     GSVVVTFSATVTGTPEFGFQWQKSPVGLNQFVNVGAAVFTTSTTATFTLTGILESDEA
                     DYRLVASNGSGTFSATSNAVTLIVNDPPTITDEPDSIVVNPGQTATFTVAATGPGPIT
                     YQWQRNFVNLTSPSATTDTLEIANAQDANEGNYRVLVSNGASVVTGPTISATATLTVR
                     DPVVITSFPAATEASNGDPVNLTAGHTGTGTPDMPNPGDPAFTYLWERNEGSGFTPIA
                     GAPNSGQLTLPPVTLADNGHQIRVTVSNSFSMDTRTTTLTVTEGTPSFLAQPESRTVI
                     AGTSVTLQITPGGSSAGRAFQWRRGKTALKNGGGITGVDTPTLEFSAIDLKQAGDYSC
                     EIKNNFGTRQSATAFVTVVAAPGTVIPVQTGKDFTLTLAFAAAKGVTPQFKWQRDGAD
                     LPVDDRISPDNLPKLVVKKSDTGAVTDSGIYTCLVTAPGPAPALNEEVAGGQFDVRVF
                     SDKPVLALEEFPAAMVGAEFEYQIKVDNDPAKAPVSYAAKPLPSGLKLDTKTGRITGW
                     PKVAVENLPVVITVTNSFSKPPVSDTKLLTIRPVPERAVGVFAGWLPRHQELNEFMGG
                     RFDMTVTAAGAFSGRVTLGAVAYSFKGVLELDPALVGAALPTATVEIPRKGKPLPPPL
                     ILTFTINTAAAPANTANRLASASIGDGTPQNTIAFNAWRSIWAPKALPPVTPAEFAPN
                     KDNAYYTLALLSPVPGAEDIPRGSGYVSFTVGADGRLTLAGRTADGQTITGAQFIGPQ
                     GQILVYQVLYKTKVKGSLLGNVSLTSAVPSELTDNKIAGTLEWNCPPNDAKTNFTYRE
                     GFGNATPVVVTAVGGHYAAPVDPNLVLGLQTGADNAVLTFENGGVEDSETNPDLDGPD
                     IQRPQQGWVSVTTGNKLLTPAAEIANNGLVKISIVSKTGVFKGDFTLGDFPAGGIKPD
                     VRKTPFIGVIVDEDDGTTAGRMWTGTGHFLLRQHPNDLPVTNPPTTLKTAPILSGQVI
                     FEPR"
     gene            complement(37436..37966)
                     /gene="vccB"
                     /locus_tag="PLP58_04110"
     CDS             complement(37436..37966)
                     /gene="vccB"
                     /locus_tag="PLP58_04110"
                     /inference="COORDINATES: protein motif:HMM:TIGR02598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Verru_Chthon cassette protein B"
                     /protein_id="HRJ07727.1"
                     /translation="MKTPLQSVTKPFHKSAGFSLVEVVLAVGIMALGVVTILGLLPHG
                     LEMSRKTANEMAEARIIEHIVGELQNTNWSILDTYGKKVRYFDDQGLELVSIASAQDR
                     FALSYVARVEIPQADVRLPTNNASLQPSNHLRRVTIRLVAAPLPDFNFDSPSSGVPVK
                     VHTQLIANMATLANIP"
     gene            complement(38182..>38457)
                     /locus_tag="PLP58_04115"
     CDS             complement(38182..>38457)
                     /locus_tag="PLP58_04115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ07728.1"
                     /translation="SADGSVELVQPDGTTQTAAPLLVADSLQHFEITLDLAQGTWLAT
                     MNGVALGDALPLPANAVFGDLSAVWYPAGGNGPVSSMTFDRVGIRSE"
BASE COUNT         7217 a        11334 c        12381 g         7525 t
ORIGIN      
        1 cggtgacggg gatggcgcgc acgggcggcg gagtgcgcag gacgacgccc gagcggctgc
       61 cgggaagcca tttgtccgcc tccatgtagc gggcgtcggc gggcagcagg gagcgcccgt
      121 cgagcacggc ggatttgcct ctgacctcca tctcggcggg cggcttgact cccgcgggca
      181 ggcgcatgtc cagcgccttg tattccttga agaagaattc cctgccgccc agcacgcgcc
      241 tgagggtgac ggttttttcc gaggcgtcca ccaccacatt gaactccagc ggcatgaggg
      301 tgaggtggtc gccgggatgg agggtggtgc tcatgaggcc gttgaggcgg atgagcatgt
      361 cggtggtggt gccctggcgg ctggcgatga gggccaggga atctcccggc tgcacgatgt
      421 agtcgcgctt ccctggaatg ggctctgtgg agaaaagctc gtccatgttg atctcgccga
      481 tgatgcgctt ggcctcgccg catgaggccg agtccgggaa ctgctggatg agccggaaca
      541 ttgcctcgcg cgcctccggg acgcggccct cattgagcat gtccgccgcc gcttcgaaac
      601 gcttcatgcc cggatcaatg cggggcatgt cggaccgttt gatctcgctc atctcctggc
      661 ggaggcgccg ctccggggcc agcaccttga tgtagatgta ccaggccacc gccatgcagc
      721 ccagcgtgac gccgagaacg atgaggtaga gcaggagttt gagctgggcc ttggccatgt
      781 cttgtcttgc ggcgggaggc gcttcacagc aggccgcgcc tcttgaagtc gaacaaattg
      841 atcttctggg acttctcgat catctcgacg gtgaacttca aaaggcatat ctcccaggtg
      901 tcgtccactc cggcaatgac ggcgcgcatg ggattgccgg tggctcggat gtcctccagc
      961 agcttgccgc agacatcgtc catggactcg tggacgacct cctcgttcat gtcgttcatg
     1021 cggaatttga agccgtattt cagcaccgcc agcttgtgca ggttctcctt gaggaagttg
     1081 ccaaaagccg cgccctgggg gccgaagccg tcgaaatcaa aatccgccat cgcggacgga
     1141 tccgggcgga ggatgagcgg cctttccgcc gtcagcaccc cctggcgcac gcgcgtggca
     1201 ttgacctggt ccatgagttc ggagactagc tggaactcga actgcgtgct gccaaaggtg
     1261 tcaatgcgcc ggtccggctc atgcaggacc cgcgtcatct ccagcgcgta ttgaatgtca
     1321 tactccgtgt gcatgggggt tgaggcatcc tagcagccgc cgcgtctttg caaagcggtc
     1381 aatttacccg tccgcgcctt ttcatccggt gtcttgcgag tgtttcagtc aggttgtcga
     1441 aaaaggggtg aagggcggca ataaaggggg gcatcattca ggaagcggtg attcataaaa
     1501 tatgaatgat gtggaaatta ttttgataat atgtggtttt tatggttaat tttaaacaaa
     1561 tcaaaacaat caccatgaaa gtcactctcc ttctctgctg ggtttcgtcc atttcaatgg
     1621 ctttgagcgt gctttcctcc tgcgctttgc cccagggcgg gtttttgaaa aaaggcggag
     1681 tgacagcagc gaagggcagt ccgctgcagc cgggagtccg ttacgaggtg cggaaggctc
     1741 tgcaggccgg cgttcctttc cgcccgcagg aaatgaagcg ctactttttt gtgatcgaga
     1801 caccgcgccc gccgatgatg gcggcggcca atgccaaagc cgggcagatg acggtgcgca
     1861 cgacggccta ctgccacgac gagtcggacc acatcgtcta tgggcgcaag aacgccctcg
     1921 gcacggacct gaagttcggc agcgtgcgca gcgcggcggc ggactggtcg cgctatcctg
     1981 tggggacgcg cttccgcatc gccgggcagc cggacgtggt ctatgaggtg gacgattatg
     2041 gcagcgccct ggtgggcaca ggcaccattg atttgtacaa gccgacccag ggcatgatga
     2101 accagtgggg agtgcgccat gtggacattg aggtgctgca atggggctcc tatgagcgca
     2161 gcatggagat catgcgcgac cgcgtgcgct ggccgcatgt gcgcagaatg gtcagcgaca
     2221 tcgagtcgaa tgtcatgcag gcgacgatga aaagcctcaa gccgcagcct ctggccgccg
     2281 cgctttgatg gcaatgagag cggctttggc aggcgctcgg caactttggc gaaagccatg
     2341 cggcgcggga tcgttcgtca gagggtcatg cgagtcctca tcctgcttat cgcttttctc
     2401 ctgccctgcc acgcccagaa ttccgcccag tcaggtccgt tgaaaaagct gcagtccgtt
     2461 ttgcaggcac cgcccgaatc cgcctcagcg accgcgctgg acggccaggt gacgcggctt
     2521 ttcggacgcc aaaatctcat gcagggcaag cctggcgcca aagtggaggg caccaccgtg
     2581 gcctgggcct tcatggacat gcagcccgcg cgcgtggtgc gcggggacgg cgaactgatc
     2641 ggtgagatga cacgcctcgg cgaggatggc ctgcaggtgc tggtgcggga gatgccgaat
     2701 ttccacgaat tccaataccg catcgaggtg gacggcagcc cgcgcgtggc gggcatggtg
     2761 cgcgtcgagc attacgagta cggcgccgac agcgagcgcc gggccggcgt gcctgaggga
     2821 gcactggaaa agttcgcatg gaacggcagc aaggtgttcc ccggaaccga gcgggatgtc
     2881 actgtttaca taccccggca ataccggagc ggggaagagg cgtgcctgat ggtctggcag
     2941 gacggcaccc gccacgcgga cccccacggg ccgatgcgcg cgggtgtcgt ctttgacaat
     3001 ctcatccaca aaaaagagat gcccgtgact gtcggcgtct ttgtggaccc cggccgcaaa
     3061 cccggccaga aagcgggtga taaagcggcc aacagaggct ttgaatacga cagcctggga
     3121 gacgcctacg tccgtttcct gctcaccgag atcctgccgg aagtggagaa gcgctttcaa
     3181 gtgaaattcc gccccggccc ggaagcgcgc gccatcgcgg gcggctcatc gggctgcatc
     3241 tgcgccttca cggcggcctg ggagcggccg gaccagtttc acaaggtgct cggctgggtg
     3301 ggcacctttg tggacatccg aggcggcaat gcctaccctt acctgctgcg cgtcaccgag
     3361 cgcaaaccca tccgcgttta cttgctcgac ggggtaaacg acctcgacaa ccagtttggc
     3421 aactggcccc tggccaaccg catgatggag gccagcctca aattcatggg ctacgaccac
     3481 cgcgtggact ggaacgagtg ctttcacggc agcaagggca tgggccccag cctgcccgac
     3541 gcactgcgct ggctctggcg cgatgtgcgc tgagcgcgcg aaccatgccc cgaccgcctg
     3601 aaaaatgcgg atggaaactg cccgccccat gaaatggatg cgggatgccc gcgctcatca
     3661 gcgctcgtca atcggcgcca ctttctgccc gtaaggccgg cccacatagg agctcagcgg
     3721 gcggaagaga cggttctcgc tccactgctc cagcacatgc gcggtccagc cgctcatgcg
     3781 ggcgatggca aagatgggag tgaacagatc cgtcggaatg tccaggctgt aatagactgt
     3841 ggcggagtag aaatccacat tggcgttcag gttcttgcgc tcgcgcatga tctccgcgat
     3901 gcgctccgac atctggatcc acttcggctc gcctagctgg gcggtgagct ggatggccat
     3961 ctccctcagg tgcggcgcgc gcggatccag caccttgtaa acacggtggc cgatgcccat
     4021 gatcttcttc ttctgcgcca gcgcgtcctc cacccatgcg tccaccttgt ccgggctgcc
     4081 gatctcctcc agcatgtgga tgacgccctc gttggcgccg ccgtgcagcg ggcctttcag
     4141 cgcgccgatg gccgcgctga tcgcggagta catgtcgctc agggtggagg caacgacgcg
     4201 cgcggtgaag gtcgacgcgt tgaagccgtg ctcggcgtgc agcacatagg ccacatccag
     4261 cgtcttctca gcctccttgg aaggcgcctc gccgttcatg aggtaaagaa agtgcgccgc
     4321 ctcgctcaag tccttgcgca ccggcggcag ttccttgccc tggcgcgcgc ggtggaaata
     4381 cgctgcgatg acgccgatct gcgagaccag gcggatggcc gtggccaggt tgtcgctcat
     4441 gtccacgtca tgccgcgcca tgtcatagca gccgagcatc gagaccgccg tgcgcatcac
     4501 atcaatcggg cgcgccttct tcggcgcgtg ccggagaaag tctatgacac cctgcggcag
     4561 ctcgcgctcg gcgcgcagcg ccgtggtgat ccggtcgagc tccgcgcggt tcggcagctt
     4621 gttgtagaaa agcagatgca ccacttcctc ataagagacc ttgccggcca gttcgttgat
     4681 gtcgtagccg caataataga ggatgccctc cgctccattg acctcgccga ggcgggtttc
     4741 tgctgcgacg atgccttcga gacctttgga gacgactgcc atgatgttcg gtggagagtg
     4801 ggggggttga actggggaaa gggaagtttg gcgtgagcgc gcgcaaagcg cgtttttgtg
     4861 gtagcctcaa gcgtgccgag gctcaactaa aatacgcccg caggcgccgg attgcgctca
     4921 tttcctgcgc atcacgcgca cccggtgcgc ctcgctgtcg ccgatgaaga cggagccgtc
     4981 cgcgtccaca taaatgccgt gcagccgcgc cattttgcac tggagggggt ccccgtccgg
     5041 gccgtcgccc ttctcgccgg tgcccgccat cagcagcagc ctgccggtct tcgcctcgac
     5101 catgcgcacg ctgtggctct cggtgtccgc cagccaggca ttgccctccg cgtctatggc
     5161 gatgcccttc ggccctttga gcgtggcctt cctggccggg ccgccgttgc cggtgaagcc
     5221 gtttttgccc gtgcccgcga tgtgcaggat tttgcccgcc ttcacatcga acttgaaaac
     5281 ctggttcccc tcccgcgtgc agagccagag attgccctcc ttgtcgaagt caatcgagcg
     5341 cggccccttc agcggcgtgc cctcgatcgg agagccgtcc ggcgtcggac cggcctcgcc
     5401 cgtgcccgcg tagcgcgtga tgatgccggt ggccaggtcc acacggcgga tcacatgatt
     5461 gccgatgtcg cagatgtaaa gatgcccgtc cgggccgaac tggatgctgt gcggcttgtt
     5521 catctccgcc cggaccgccg gaccgccgtc gccgctgtgg ccgggcttgc ccgtgccggc
     5581 gatggtgctg atgacacccg tcctcatgtc caccttgcgc accgcgtggt tggccatgtc
     5641 cacaatgtaa aggtccccct tcgcatcaaa gcggatctca tgcgggagat tgaatgccgc
     5701 ctccaaggcc ggaccgccat ccccgctgta gcccttctgc ccgctgcccg cgacgctgtg
     5761 gatgacgccg tccggcgtga tcttgcggat gcgctggccg gtgtattcgc agtaccaaat
     5821 ggctccgtcg gggccgcgaa tgacgccaaa cggattgtcc atccgcgcct gggttgccgg
     5881 gccgccgtca ccgctgaaac ctttttcccc tgtgccggca aaggtctcga tggtccattc
     5941 ggcggcatga gtctggagag cgagcgcgaa cgcgcagatg agcagggtga tggtcttcat
     6001 ggtggtggat gagtgcccgg acaaaacgcg ccccggcgcg gaaagcaatc ggacgatgcg
     6061 agcgccgcct ttcgccatgc atttttgggg agccccatcg tttcgccaaa agtattcccg
     6121 cgccgcagtg tgagctgtca atggatgccc cggcggagcc tgagtgtttt tcctggaaca
     6181 gcacgaatcc gtgacggata gcgaaatggc cttggatgag tgtgtttgcc ggagcattcc
     6241 ggcgccgcgc gaaaacattc atcgcagtca tggattcttt tcatcggagc cgtgatgaca
     6301 tggggcttgc gcgctggcag gcaagatgct tcttggcggc tcatgagcac gacaaacagc
     6361 ctctggaccc gcttccaaaa acacttcctc agacatccgc agaccggttt ctccatggac
     6421 gtgagccgga tgaatttcga cgaggcgctc tttgatcaaa tggagccggc catcacccgg
     6481 gctttaaccg ccatgcgtga gttggaggcg ggagccatcg ccaatcccga cgagagccgc
     6541 atggtcggcc attactggct gcgcaatccg ggcctggcgc ccaatgagga catcaaggcc
     6601 gagatcatcc gcaccctgga cgccacactg gccttcgccg cggatgtcca tgccgggaaa
     6661 atcctcgcgg ccaacggcca aaaattcacc cgcgtgctcg tcgccggcat cggcggctcc
     6721 gcgctcggcc cgcagctcgt ggcggacgcg ctcacgcccg cgaacccgcc gctggcgatt
     6781 gatttcttcg acaacacgga cccggacggc atggaccgcg tcaccgcccg catcggcgcg
     6841 gagatcgcca ccactctgac gctggtgatt tcgaaatccg gcggcaccaa ggaaacgcgc
     6901 aacggcatgc tggaagccgc cgcagcctat gcggcgcggg gcgtggactt cgccaggcac
     6961 gccgtggcga tcacgggtga cggtagcgag ctggacaagc acgccatctc gcagggctgg
     7021 ctggcccgtt ttcccatgtg ggactgggtg ggcggccgca ccagcgtgat gagcgccgtg
     7081 ggcaccctgg cagccgcgct ccagggcgtg gatgtgcgcc agttcctggc cggcgccgcc
     7141 gcaatggatg ccgagacgcg caagcgcccc gcgcgcgaaa acgccgccat gctgctcgcc
     7201 ctcatgtggc accacgccgg cggcggacgc ggcgccaagg acatggtcgt gctgccttac
     7261 aaggaccggc tcgtgctttt cagcaaatac ctccagcagc tcgtcatgga gagcctgggc
     7321 aaggagctgg acctcgacgg cagggtggtc aatcaaggca tcgccgtcta tggcaacaaa
     7381 ggctccaccg accagcacgc ctatgtgcag cagctccggg atggtgtgaa caatttcttc
     7441 gccgtcttca tccaggtggg ccgcgcgcgc gacacggcgg gcatggaggt ggagcccggc
     7501 tacacgagcg gcgactatct ccagggcttc ctgcgcggca cgcgcaaggc gctggcgcag
     7561 agcgggcgcg agtccatcac gctcggcatc agcgaactga acgccttcag cctcggcatg
     7621 ctcgtcgccc tcttcgagcg cgcggtgggc ttttacgcca cgctggtgaa tgtgaacgcc
     7681 taccaccagc ccggcgtcga ggccggcaaa aaagccgcca ccgaattcct cgcgcagctc
     7741 ggccaggtgc gcgccgccct gcccgcctcc ggcgcgggcg tcacggcgga ggagatcgcc
     7801 ggcagcctgg gcattgaccc cgaggacgcc tggcacatgc tcacccatct ggccgccaat
     7861 ggcgcggcca aaatcaccat cgcgggagac actccgggtg gcgaccgctt cacagcagca
     7921 ggctgagtcc gccgaatggc ggtgaagttt gcggagtccg cgcatgaggg acttttgccg
     7981 ctgggccggg atcaatcctt cggcacatgc gggacgagaa ggtcgggcgc ttgattgcgg
     8041 gcgcgggcgc ggcgggctgc ctcctgggta aaggagagct gggagcggac ggccctggct
     8101 gcgggaacgg cgggctggat ccatgcgcca tcggcgcgga gatggcgtcc gcgcgaggtg
     8161 tcggcaaagt gggtttcgag gatgtgggtg aggcgctttt tggcgtcctt gtcctcgacg
     8221 ggcacgagca gctcgacgcg cttggagagg ttccggttca tgaagtcggc gctggagatg
     8281 aagacgaccg gcctgccgcc ctggcggaac cagtagatgc gtgcgtgctc gagatagcgg
     8341 tctatgatgc tgacgacggt gatgttgtcg ctcacgcctt tgacgccggg gcgcaggcag
     8401 cagatgccgc gcacgttgag gcgcaccttc acaccggcct gggaagcctc gtagagggcc
     8461 tcgatcatgc ggcgatcctc aagggcgttc atcttcagca tgatggcggc ggactcgccc
     8521 tggcgggcgc gctcggtctc gctgcggatc atggaaagca ggcgctcgcg caggccgaag
     8581 ggggccatgc tgatgcgctc aaagtggacg aagcgggagc ggccggtgac ggtgttgaaa
     8641 aaggcggagg cgtctgcgcc gaaaccgggc cgcgcggtga gcaggctgat gtcggtgtaa
     8701 agtcgcgcgg tggactcgtt gtagttgcct gtgccgaagt gcgtgtaacg cacgagatgc
     8761 ccggcctccc ggcgcatgac gagacatatt ttggcgtggg tcttcagccc gcgcacacca
     8821 tagatgatct gggctccggc attgagcagg tcctcggcgc gctccaggtt ccgcgcctcg
     8881 tcgaagcgcg ctttgagctc gacgaggacg gtgacatgct tgccggcctg tgccgcgcgg
     8941 atgagcgcgc tgatgatgcg gctgttcttg gcggtgcggt agaggatctg cttgatggcg
     9001 atgacgtttt catcggcggc agcctcctcg atgagctgga gcactggatc aaagctctcg
     9061 aagggatggt gcaggaggat gtcgccggct ttgaccgcct cgaagatgct ctggccgggt
     9121 tcgatctggt gggacggatg ggtttcccat gtctcgacct tcaactcgtc gaagccggag
     9181 agggaggcga tctgaaaaca ggccctcaaa tcgagctcgc ctggaatggt gaaaacctgg
     9241 gcggtgcgcg ccccgcagag gtcgcggatg attttgacta ggtcgcgcgg ggcgccctgc
     9301 tcgatttcaa tgcggatggc ggggctcagc aggcgggcct ggaggacgtc ctccatctcg
     9361 ctggcaaggt cgaaggcgct ctcatcgtcc acggcgatgt cgctgttccg gctgatgcgg
     9421 aaacgggcgc agtcggtgac tttctcggag gggaaatagt gctggagaaa gagttcgatg
     9481 aggtcctcca gattcacata gcaatgcgcg gcggtctcgg catcggggac ggtgacatgg
     9541 cgtgggaggt tcgagggcag ggtgaacaga acgtggcggc ggctggcccg cccgtcctgc
     9601 tcggagagca cagcgcagag gagggcgatc tggagcgctg gcacggtgat gcgcggagcg
     9661 tcctcgtcga gcgcgatggg cgagagcacg gggaaaatgt gctcgttgaa gtggtcctca
     9721 aggtgcgcgc gctgcgcggg agtgaggtcg ctgacgcgca ggcggcggat gtttccctgc
     9781 cagagaagcg gacgcaactg cccttcaagg atttcatact ggcgggcgac gaaggcggtc
     9841 gcccgctcgt ggatgcgctc ccattgctgt gtgggagtaa ggccggcggc gtccttgtgg
     9901 cgcatgccct gctcgcgcag aagctggagc gcgccaacgc ggaccatgaa gaactcgtcg
     9961 agattcgagg cggtgatggc gaggaatttc agccgttcga gcacgggcag atcacgccgc
    10021 gcggcctcct caagcacgcg ctcgttgaag gccagccagc tcaactcgcg gttgaaaaaa
    10081 tgctcctcgg tgacgggaac aggcggtgtg ggtgtgggca tagggcatga ttgcccgcgc
    10141 gcctgcgcgc gcaagtgacg ttgcgcgcgt cactcccatt cgatgctcgc aggcggcttg
    10201 gtgctgatgt cgtagagcac acggttcacg cctttgacgc tgttgaggat cttgttggag
    10261 gtgctgctaa gcacttcata gggcagccgg acccattcag cggtcatggc gtcctcactg
    10321 atgacggcgc gcaggctgat ggcctgctcg tagctgcgtt cgtcgccttt gacgcccacc
    10381 gttttaaccg gaagcagcgc ggcataggcc tgccaggtct gctcatacca gccgctgcgg
    10441 cgcagttcat tgatgaagat ggcatcggcc tgctgggtga tggcgacgcg ttcgggcgtg
    10501 atctcgcccg ggatgcgcac ggcgaggccg gggccgggga acggatgacg ccacagcgca
    10561 cgatgcggga tgccaaggct ggcaccgagg gcgcggacct cgtctttgaa gagctcggcg
    10621 agcggttcga gcaccttgcc ctgctctttg agctccatga tcttatcgac gcggttgtga
    10681 tgcgttttga ttttggaggc aatgctgccg ctggtggcgc tctcgatcac gtcgggatac
    10741 aacgtgccct gcgcgaggag ttcgacgttg tcggccacct tccagaactc ctcgacgaag
    10801 agcgtgccga tgatgcggcg cttttgctcg ggatcggtga cacctttgag cgcaccgagg
    10861 aagatggcgc tggcgtcgat ctgctcgatg ggcacgccga cttcatcaaa cagcgttttg
    10921 acctccgcgg cctcgttcag gcgcatcatg ccggtgtcga tgaagatgca gcgcaccttc
    10981 acgcccgcct tggcgagcaa cacagcaagc accgtgctgt ccacgccgcc ggagacgccg
    11041 cagatgacct cgcgattgcc gacctcggcc ttgatgcgct cgagcatctg cgccttgaag
    11101 gcattgatgt cgaacttggc gagctgcgcg ccgctcttgg tgaggaagtt cttcaggatg
    11161 gcggtgcctt cgtgcgaatg cgtgacctcc gggtgaaact gaatgcccca gcaacgatcg
    11221 gaccatttca gggccaccgg tacggcgtcc tcattcgtgg cgatgacttt ggtggtgctg
    11281 gccagatttg cgcaggtgtc gctgtggctc atccagacct gcgattcgtg cgagatgcta
    11341 ctgaaaaggt cctcatggtc agtgacgacg agtttggcgg gtccgtactc gcgggtgacg
    11401 ccgggcttca ccgtgccgcc gtgcttgatg ttgaggagct gcatgccgta gcacacgccg
    11461 agcaccggca cgccgaagga catcagtttg tcgtaatcga catcgggcgc gtctttttcg
    11521 gaagtgctgc gcgggccgcc ggaaaggatg atcgctccag ggttctggag gttcggcagt
    11581 tcggcgggcg gatacaggtg cgaggcaaaa cccagctcgc gcacgcggcg gacgatgagc
    11641 tgagaatact gggagccgta atcaaggacg gcgacttcgt tttcagtcat gggcgggaca
    11701 gcatggaaat ggcaaaggag gctgggtcag gagggctcgt agttcaccgg ttcctcggtg
    11761 atgaccacat catgcggatg gctttccttg agtccgccgg cggtgatgcg gacaaaccga
    11821 gccttggcgc gcagctcgtc gagattgttc gcaccgacgt agcccatccc gctgcgcaga
    11881 ccgcccatga gctggaagac gacatccgaa agcggcccct tgaacggcac gcgcgcttcg
    11941 accccctccg gcaccagctt gccggagctg ttctgtccgt agcggtctcc agcccctttg
    12001 cgcatggcct tgaggctgcc catgccgcgg tattccttga aggtgcgccc ctgccatttg
    12061 accatgtttc caggactctc ggcggtgccg gcgagcaacg agccgagcat gacgcagtcc
    12121 gcgccgccgg cgatggcttt gactatgtcg cccgaatagc ggatgccgcc gtcggcaatg
    12181 acggaaacgc cgcgcggacg gcatacagcg gccacttcgg tgatggcgct gaactgcgcc
    12241 atgccgacgc cggagatcac gcgcgtggtg cagatcgagc cgggaccgac gccgaccttc
    12301 acagccgacg cgccggcctc catcaaatgc agggcgccgt cggcggtgac gacattgccg
    12361 gcgacgaccg ggatggagcc gccgagatgc tcgcgcaacc gccggaccac ttcggccacg
    12421 cgggtggtgt gtccggtggc ggcgtcaatg aacaaagcat ccgcgccggc tgcggcgagc
    12481 gccgcgccgc gctccacgca atcctccccc acgcccacgg cggcaccgac acggagctgg
    12541 ccgttggcgt ccttggcggc atgggtgaaa gtctggcgct tcacgacatc ctgcttggtg
    12601 atgagaccag ccagctttcc gtccgcgttt atgaggggaa gtttttcgat acggtgggtg
    12661 tacaggattt tgagcgcctc ctcataactt gtgtcggctg tgccggtggc gagtttgccg
    12721 agaggtgtca tcatcgcgga cacaggcgtg gactcatcct ctatgtacca catgtcccgg
    12781 ctggtgacca tgccgacgag cctgccctca ggatccacga ccgggaaacc gctgacgcct
    12841 ttgatgtgca tgaggcgctg caattcgcca agcgtcgtgc cggggctgac agtgtgcgga
    12901 ttttgaatga cggtgttctc cgagcgcttc acccttgcca cctgctccgc ctgatgggtg
    12961 accgggcagt tgcggtggat gacccccatg ccgccttcac gcgcaagagc gatggccagt
    13021 tcggcctcag tcaccgtgtc catggccgag gaaagcacgg gtatgcgcag gtggatcgaa
    13081 ccgccgaaag gagtgccaat ttccacttct ccaggcaaga cctcgctcag gcccggcagg
    13141 agtagaacat cgtcaaaact aagcgcaagg gaaggtatgt cgcccatgtg ccataaaagc
    13201 ggcacatccc gtcttgtcat gcggaaagtg cgccggagct cagtcagggc cggtaaacac
    13261 gcgtcttgcc ggtgtaggag tcaatctgga cgcaaaaata attccgtggc tgggcgctac
    13321 cggcatcctt gctctccacg atggtcagaa aactttcatc gtagggaggc acggtgtaat
    13381 ccagggccac ggcctgcgcc tcatcggtgc tggaagatcc tttgagcatc ttcatggagc
    13441 cgtcgggaaa gaatctcatc gccgcatagc tcacattttc aggttcggtg cttcccggcg
    13501 ggcggaaaag aaaatttccc gcatcgtccg gcctttgccg gttcgttgtc tgcaaaagag
    13561 gagacaactg ctgggtgcgg gagaggatga ttccagactc cagataatag gcctcggaaa
    13621 gggagcgttc cacgcccgat tgcggactat gcaccacctg gtaagcataa aacagatcgt
    13681 cccccggcct gtcggaactg ctgttgatgt agctgtaaaa gcgcatctct acctctgaac
    13741 ccatggaaat ggcgctctgc tgagccaggc tcaaccggtt gatcagggaa tcccccgaag
    13801 aactgagacg tgtcgagcgg atcacatcca gcagcgtcgg cgtgacgagg gcgacgagaa
    13861 gcgcgatgat gcccaccact gtgatcatct cgatcaaagt gaatgcggcg gctttgtttc
    13921 ggcggagtgg agtcatggga gagggcttgg acgcgcaggg ttcaggtgct ccacttggcg
    13981 ccacgcagcg ccaccgtggt ggaaaagatg cggtaattga cgcgaaactc ctcgcgaaga
    14041 accctggcca cttcctcaag cgactggttc agttgtgagg cggggctgcc gttgataatg
    14101 tcgtggatct gggttccgaa tggtggcgac tggccatccc cgccgccatt ggtttgcaca
    14161 agcctctccg cagaacgctc gtcaatcgcc accagcacca ccctcacgag tggaggcagg
    14221 aggtattgcg tgccctgctg tcccatgccc gccgagttcg ccagcctcac ctcatccgaa
    14281 tcgtaggcga agttctccgc gatgactgtg gaggatgcgc caccgcccgc ggaaccagga
    14341 tcaagcatgg gggatatgac cagtgcgaca atgttgtcgg cgaccggccg gttcatgccg
    14401 cggttgatcg aagtttcatt gccgccgcct ccgcttctgc ccgcagaatc ggtcaggctt
    14461 ctgcccgcgt cctgcatcca ggcgttccgc tggttggcat aaatgatgct gcgttcagag
    14521 ggaggggaaa attccatgag ccgataacgg tacttcaagg tgctggatgt gatgaaaccc
    14581 ggccggaaaa actcgtcaga gctgtattgg acataataac cgcgtccgca aagcagccgg
    14641 tccaaaccgg cgaactgggg atcatgagtc acccccagcg gcgcctgaaa gaacacggca
    14701 ctcccaggca ggactgacgc gccccccgtg ggaatgagag tcgaggcggg accgcagaca
    14761 aaacgcagct cagttttccg ctggtagcgc gtcggagcct gggtggtggc cgccactgga
    14821 tccgccgaat tcagataatt ggtgtcatag tccacataag tgttgagcgt ggcctgactg
    14881 atgttgcggg tgatgatgtc gaaggcggtg cgcgcctccc tgaactggtt gacacgcgca
    14941 ttggtggcgg tccaggtggt ctgcacagtg ccgatgacct cggcgctgat gagcagcagg
    15001 atggcgagca cggcgaggga gacgagaatc tccaccaggg tgaaggcgcc ggagaaaacg
    15061 cggaggatcc tatcggcagg aggatttggg gatgatttca tggggggctt gtctgagttg
    15121 tggcatcagg gagcgaagcg cttggattcg atgaccctga accggtaata gtcttccagc
    15181 ggcctgagcg agaacggatc ggaagcgttg gcataatcca ccgcattgcc agccctctgc
    15241 aggtcaccct ggtcaatgta gcgctcgacc aggaaagaac cgcgaaactc gccggtcatc
    15301 acatcgcggt cgggatcaaa ctgcgcgggg tccacggaac gcaacgactt gcggatggac
    15361 tgggcgcgca catgcacacg gaaggtgttg gagcgcgtgg tgaggcgcgc gtagaggttt
    15421 gaatacggac gttcgcgggt gttgtcaccg gtggagcaat gctccgccca gaagcgcccc
    15481 atcgcattgt ccctggcatt gacgtccgtg acatcgccgg gcgacacatt cacggcagct
    15541 ccgccgggag cgggattggg gatcaaatgc aattcgcata tctgggaggc cgtgcggaac
    15601 agtcctcccc gcattccctc gggcagagtg gagttgttct ggctgagacc aaaattgaag
    15661 cgttcctcaa attggcgcaa agttccctgc acacgctggc caatggcaag ctgccaccag
    15721 cgctggtcag cgccactggt cggcttgaag cggtcaatgg caatggtgcg gtgccagtag
    15781 gcgttgcggt tcgccccggc tccagtcgcg gtaagcggca gggactcgtt gcgcatgacc
    15841 ggcgccgtgc tgccattggt cccccagccc gttcgaacat ccttggacaa ttcataatcc
    15901 gtgttgggca gggctgcgaa gacctcccct ttcatgaccg catgcacggc ggtggccctg
    15961 cggatgtggg tgaagggcat catctggtag ttcatgttga ttttgcccgc tgtggaaagc
    16021 gactcgctga tggcgtaagg ctccaccaca ggcatccaga agaggtcgag caaataatgg
    16081 tccggtggag tcgtctcgcc tgggtgcgtg gcatccgaag tcaccccgcc ggtaactccg
    16141 ttcggcaatt ggacatgggg ccggaacagg gcattcgtcc atgctccctc gccattgtta
    16201 tcccacatcc tcgtcggcag ggagccgaac atcactggcg aggagatcat gcggttggga
    16261 gtgaaatagc tgccctgacc cgccgccaca cgcgacccct ggccattcga gcggaagtat
    16321 gtggcgcgcc acttcttcgg ctggccgggt gtccagccag aaatccagaa atctccaacc
    16381 gcgtaatttc cctcatccgg tttattgatg aaggaaccga cacgcccgcc gggatcactg
    16441 tcgtcaaagt cataataacg ctgaacgaac gcatgggcag ccctgctggt cggcgccaat
    16501 gtgctgcgac cgtggggggc atcggggctg cgggctgcgg aaaccgtcac atccggaggc
    16561 agcgcgcgga cggtgttgtc caccagattc gagaaactgc tggtgccgcg gtcaaacccc
    16621 ggctcaccac ccgccgtgta ggaggaaaaa ttgtgcgcca tgtactcatt ggcatcattc
    16681 cagaagcggt gcggggtcca atcggcgggc tcaaccaccc ttttcacaaa gaggatgcgc
    16741 gcgtcgccat gcgcgggttg cattgcccga accacatccg agccaaaatg ccagggtcgg
    16801 tcccacgaag attgcgggtc accaggccgg gggccatact tgatgcggtc cgcgatgaac
    16861 ccagggtcca aaagcctgac ccccacataa ttgccggaat ctctcgcgta aatcaaagcc
    16921 ctggcaccag gcacgctttg agcattcgct gtggcgattc cagagccaga atcaatagca
    16981 ttggggttat gggctcgggt ttcggtgtca acacgctggc cagcaaaatc cctgttgaat
    17041 cttccttttt cggaatccac ggcaattccg agagacctgc cgagcacacc atcacggtgg
    17101 aacccccacc agcgcggcgc ctgcaaaaga ggatgattgt attcagtgcc gtcgggtcgg
    17161 atgaaattca cctgataaga ccccgcgcag acaagatccg gagtcggtgc ctggccagcc
    17221 gacaggcgga aacggacgac ctgtaccggg gcggcgctct gccagtcgtg agtgtcgtaa
    17281 atattgaccg tgatcggcgg cgaggagaac tgaagcgcgc gattcctgtt cacagtaaag
    17341 aaactcgaca ccaaatccat gttgagcacg cctgagtgaa cgttcgaggt cgcattcgta
    17401 tcgtagcccg tgtcgtccgg cacccgcccc atgcccttca cgcgccgtcc caatgaaatt
    17461 ttgcggaagc tggcgaatcc tcccacctcg ggagttccat ccgtctcaaa caaaggcctc
    17521 tcggtgcgaa tgaccacatt ctgcgtggtg ctgaaaatgg cctttccatc cacttcaatg
    17581 ttgctgatgt cattggcgga gatgacaaag gtcatgtcgg ggatgatttc cccataaccc
    17641 acggacgggc agaaaaactc caggtgcagc agcgcctgaa cgcgcttttc atccactttg
    17701 agcggggtgt cctcctccag ggaatggttc cagttttccg ccctgtatcc cgggtgtctg
    17761 gcgggctggc gcgtgaaatt cttggtccct ggcccgttcg gcgggaggtt ggtggtattg
    17821 tacaaaccgc cgctcgcata agtattgagg ggcggaaagt tggaataata gcggtccttg
    17881 atggacctgt ccccgcgccc ggcccagcga acaccgtcca ggttggtgat tgcaacctcg
    17941 tccatcgcca tattgccgag aggcagatcc ctgatggtgt aggtgttttg gaaggccaca
    18001 ttttcatcca atttcagggc tgtgcgcccg ccgctcacat taggcacatc cgggctgctg
    18061 gcgaagtaat ccacataatt gaccaaatcc tcatccgcga tcaccggaat ttgatagtca
    18121 gtcgcggcgg ctccgccctc ggtggatttc attggaatgc gccaactata catgtccggc
    18181 tgcccatccg ccgtgcaaat gaattggagg ccgatttcgc taatcgagac gaagcgtcca
    18241 aagccgcgat aggttctgcc tccgacagac cactccgcag gcgtgacctg gccgtgacca
    18301 ggaagaaagc ggtcttcgaa cggatcgttg atccgggctt gcgagcggtt gccgtcccta
    18361 gtggagttgg ggttgcgagc aacaccgtct gtgtaagtcc ttgtctgcgc aaccagcctg
    18421 tcccgctcgc ggtaggtttg cgtccagtcc accgtgctgg cagtgccgga tgtgacaaaa
    18481 gtgggccagt tattgcggtt ctgcggaatc aaataactgt cgtacagatt ggtggaacga
    18541 atgtaatcga agatttgcac cagcagttgg cgtgtgttgt cctgcccgta tttttgcaca
    18601 taggatcttg ccacggcccc gcctgcgcca tccactgcgg cgggaaagga tttgttcatg
    18661 agggcgtcca gcatcgccat gagtctctga ttgcgctgga tgttgatgtc ataactcgcg
    18721 ccactggagc ttggcggaat ggtgcgcgca aaagtgcgct ggaagatgta gttgttgccg
    18781 gccgatccca ggcgggtgca gaactcgatc aaattgtcaa aaccagtcct gcggtcctgc
    18841 ctctccgaca ccggccacat ggcgacgcgc gggaggccga acatgttcac ctcgctggcg
    18901 cggctgtgag cggtaaggaa agcggaagat cgctccaagc tttggcggtt gaagagagta
    18961 gtgctgcccg cgaccgcgtt gttcaaaacc ctgcgcctgt cggaatccct ctgggagaag
    19021 agcagttcgt caacactggc gtagagacgc tcgcccgccg cctgctgaat ggcgaccgct
    19081 gtggcattgg tcgaatctcc agaaagggtg cgataagcgt cctgttcgaa gctgcgcgtt
    19141 ccggaaaggc tgccaccagt atgaatgcgc ggaatcaaat catagagcct ctctttgacg
    19201 gcgagcgcgt tggaaagcgc cgtgccggtg atgcgacccc tgggaccgta cgtgtcgagg
    19261 ctgcggttgt cagcgagagt cggattggga tagagaacgc tgctgagggc aaccgtggcc
    19321 gggtggccgg gaaagcgctg gtactcgctg cgggccgggg gaaagtccgc ccaggactgg
    19381 tcgcgctcgt gatagtagat gggctggccg gtgtaggtcg gctcggaagc tgtgttgatg
    19441 ttgactttgc agctctcgtc atccgtccag aatgcaacac gacccacgat ggggttggtc
    19501 tctgaaggca ctccatactg gtcctgcggc gaactggatt ccccgctgcc caaagctccc
    19561 gaactacccg ccaaaacctg aaaacgcaaa gtttcatcca gataaccgac agctccatcc
    19621 cgaagtatat agagccattg aaccggcatg gcgagacgga gggagtccac cgatccgccc
    19681 gccgcgcctg gcagcacaat cgctctctgc tccggattga tgtcggaggc cccggaaaga
    19741 tcctggtttc tgagcgcggt cgcagtgttg taagagaagc cttccaccgg aaattcatcc
    19801 cctggattgg ggtcaatgtc ctgcgcggcc cgaggatcca ggaccggaaa gatggtcagc
    19861 gtcgtggttg aatttcccga cgcgtcaagc tgacccttga tcacaggctc gttgatgtcc
    19921 acaaaacgcg cgatgttgac atcccggttc caattcgccg gaggttcgga attggccaca
    19981 aactgctgct cgaattcgtt gggattgctg gcactggctg gaggcgagcg gaaaatcatg
    20041 tccttgtcgg aaaagagctt gtaaccggcg atgaagattc cggtctggct gtattttctc
    20101 accgcccccg gctgggtggc gtgaatcacc ggggcagccg cagtttcccg ttcacttccc
    20161 gcgcggattt gcgagacaac catgttgacg gctgtgtccg caagccgccg ggcggtctgc
    20221 gatgatgaat aagtgatcgt ggctttgtgc tcggtgtcag cgacactgag gaaggccagg
    20281 atgacaatgg tcgccagggc gagcattgac agcacgatga tcaaggcgag accttgatgc
    20341 cgccgggcgt cggcgaggat ggagcggagc tgtttcatgg cggtgatttc agcgaattat
    20401 tggttttttt cgattaaacc ggaaagcgaa acgcatttca agtcaatgca gagtatttac
    20461 ttggttatta cagaagattt ttttgacccg gaacggctcc gtccgcgatt tgggaagtga
    20521 cgattcatct tggctcgaag atcacctgac cggagagaat cggcgcggtt ttcaaggtgg
    20581 tgggcggatt ggtgaccggc agatcgttcg gatgctggcg aaggaggaag tgtccagtgc
    20641 cagtccacat cctgccggcg gtggtgccgt catcctcgtc cacaatgacg ccgatgaagg
    20701 gtgttttgcg gacatcaggc ttgattcctc cggcggggaa atcccccagc gtgaagtcgc
    20761 ctttgaaaac gccggtcttg gacacaatgc tgattttgac aagaccgttg ttggcgattt
    20821 ctgcggcagg cgtgagcagt ttgttgcctg tggtcacgga aacccagccc tgctgcggcc
    20881 tctgaatatc tggaccgtcg aggtcggggt tggtttcgga atcctcgact cctccattct
    20941 caaacgtgag cacggcattg tccgcgccgg tttgcaagcc gagcacaaga ttggggtcaa
    21001 caggagcggc gtaatgcccg ccgaccgcag tcaccaccac aggcgtcgca ttgccgaagc
    21061 cctcgcgata agtgaagttg gttttggcgt cattcggcgg gcagttccat tccagagtgc
    21121 ctgcgatttt gttgtcggtc agttcggacg gcaccgcgct ggtgagggag acattgccca
    21181 aaagggagcc tttgaccttg gtcttgtaga gaacttgata gaccaatatc tgtccctgcg
    21241 gcccgatgaa ctgcgctccg gtgatggtct ggccgtcggc ggtgcgtcct gccagggtca
    21301 ggcgtccgtc cgctcccaca gtaaaggaaa catagccgct gccgcgcggg atgtcctcag
    21361 cgccgggcac tggagacaac agcgcaaggg tgtagtaggc gttgtccttg ttcggagcga
    21421 attcggcggg tgtcactgga ggcagcgcct tgggcgccca gatgcttctc caggcattga
    21481 aggcaattgt gttttgcggc gttccatccc cgatgctggc cgaggcgagc ctgttggcgg
    21541 tgttcgcggg agcggccgct gtgttgatgg tgaatgtcag aatgagaggc ggcggcagag
    21601 gcttgccttt gcgcggaatt tccaccgtgg ccgtgggcag tgccgccccc acaagagcgg
    21661 gatcaagctc cagcactccc ttgaaggaat aggcgacggc accgagtgtg actctgccac
    21721 tgaaggcgcc ggcagcagtc acagtcatgt caaagcgccc tcccatgaac tcattcaatt
    21781 cctggtggcg cggcagccag cccgcgaaaa ctccaacagc gcgctcaggc acggggcgga
    21841 tcgtcaggag cttggtgtct gaaaccggcg gcttgctaaa actgttggtc acggtgatga
    21901 cgaccggtag attttcgacc gccaccttgg gccagccggt gatccgaccg gtcttcgtgt
    21961 cgagtttgag accggaaggg aggggtttgg ccgcgtagga aaccggtgct ttggcgggat
    22021 cgttgtccac tttgatctgg tactcaaatt cagcacccac catcgcagct ggaaattcct
    22081 ccagggccag gacgggctta tcggagaaga cgcgcacatc gaattgaccg cccgccacct
    22141 cctcgttcaa tgccggagca ggccctggcg cggtgacaag gcaggtgtaa atgcccgagt
    22201 ctgtcactgc tccagtgtcg gattttttga ccacgagctt cggcaggttg tcgggggata
    22261 tgcggtcatc cacaggcagg tccgccccgt ccctctgcca tttgaattgc ggagtcacgc
    22321 ctttggcggc ggcaaaagcc agggtcaggg tgaaatcctt gccagtctgc accggtatga
    22381 cagtgcccgg agcggccacc acggtcacaa aagccgtggc gctctggcgt gtgccgaagt
    22441 tgtttttgat ttcgcaggaa taatcaccgg cctgcttgag atcaatggcg ctgaactcaa
    22501 gcgtgggggt gtcgacgccg gtgatgccgc cgccgttctt caaagcggtc ttgcctcgcc
    22561 gccactggaa ggcgcgcccc gccgaggaac caccgggggt gatctgaagc gtgaccgatg
    22621 tgcccgcgat caccgtcctg gattcgggct gcgctaggaa gctcggcgtg ccctcggtga
    22681 ccgttagcgt ggtggtgcgg gtgtccatgc tgaagctgtt ggagaccgtg acgcggattt
    22741 ggtggccatt gtccgccaac gtgacgggcg gcagcgtcaa ctggccgctg tttggggcgc
    22801 ccgctatcgg agtgaagcct gatccttcat tgcgctccca gagataagtg aaggccggat
    22861 cgccggggtt cggcatgtca ggcgtgccgg tgccggtgtg gccggcggtg aggttgacgg
    22921 gatcaccatt gctggcctcg gtggcggcgg ggaagctggt gatgaccaca ggatcgcgca
    22981 ccgtgagtgt ggcagtcgcg ctgatggtcg ggccggtgac gacactggcg ccgttgctga
    23041 cgagcacgcg gtaattgcct tcgttggcat cctgggcgtt ggcgatttcg agggtgtccg
    23101 tcgtggccga aggggaggtc aggttcacaa agttgcgctg ccattggtag gtgatcggcc
    23161 cgggaccggt ggcagccacg gtgaaggtgg ccgtctggcc ggggttcaca acaatcgaat
    23221 cgggctcatc cgtgatggtg ggagggtcat tcacgatcag ggtgacagcg ttgctggtgg
    23281 ctgagaatgt cccgctgcca ttgctggcca cgagccggta atccgcctcg tcagactcca
    23341 aaataccagt gagagtgaaa gtggccgtcg tcgaggtggt gaagacagcc gcgccgacat
    23401 tgacgaactg gttcaagccc accgggctct tctgccattg gaagccgaac tctggcgtgc
    23461 cggtcaccgt ggcggagaag gtgacaacga ccgatcctcc cggattcaca gggctgccac
    23521 ccgcaggcac ggtgatgacg ggatgagtgt tgatctgcac cggcgtggtc aggccggtgg
    23581 atctgatggt gccctggctg aggaactggg aagtgtccca tgagacatga ccgggcagga
    23641 cgaggtggtc gcgcgcgttc gccccgcctg gcagattgat ggtgccatcc accaggtcaa
    23701 ataccgtgtc cacggccggg atgaagccgt caagcaactg cacaaggaac tgcccggtgc
    23761 cggtgaccgt gaccgaattg gcgaccagcc gggtgatgtt cgcggctccg atggcgaact
    23821 cgaccacgga ttccggcccc acagtcagag gagcgttgat cgtcagggtt tccacagccg
    23881 aaaccatggt ggggtctccc ggacgaagga tggccttgtt gccaaccgac cccgtcagag
    23941 tcaccgcgcc tccaatgaaa cccgtgccgc caaggactgc ccccgcattg accgcgaccg
    24001 ctcccgaacc cgtggcggaa cccgtggtgt tcaataccag tagagtgccc tgctgcactg
    24061 tgacgccgcc aaagaaagtg ttggcattcg tgagactggc ggtgcccgcg ccagacttgc
    24121 tcaaggacat ggagtcctgg ctggcgccat tgccgccatt ggactcggag aacaggcctg
    24181 agaaggtgat gccatagcca gtgttggtga aggactgcaa gcccaccgtc ttggtctccc
    24241 gcacgccggg cagacggttt tgcagcaaga ccgccccgct gaaggtggag ttgcccgagg
    24301 tgaagtccgc catgccgccc aggaccgcca gtccggagcc gttgccggca ttgacatcaa
    24361 tgctgttgga gatggtaagg ccgccggtgc tggcaagcag cgccgcattg gagtccgcat
    24421 agtcgcgaat ccactgcacg gcggagacgt tcagctcaag caccttgctg gcgatgaaga
    24481 acgcctggcc ggcatgggtg ccgttgagca cgcgcacctg ggtgccgtag gccagctcgg
    24541 ccacgctgtc catctgcgcc acacgcgcga ggttcatggt gccgtccggc tggaggtctc
    24601 cattgaagat gatctgataa acaccgttcc actgcggatt ctcccgctcg tccttgacca
    24661 ggatcagggt gccctggtca tcctcggtgt aagtgcgccc gtcaatggtg atgtccactt
    24721 cgacgaaggc gccgggaccg cccgcgttgt caaagatgcc gttgtggtcc gcgtcaaaac
    24781 ggcccttcag cgcggccagg ctggcgaaag tggtggctcg ctcgacggtc atgatttgcg
    24841 gggtcgcgtc gcccatctcg acagttccct ggccgatcgc cccattcgct cccacagcca
    24901 gagtgccggc gcgcacgatc accgccgagt tcacatcggg tccgggtccg ggcagggagt
    24961 cgccaaaatt gtttcccgcg ttggtcagca gcaaagtgcc ctgggctgac agagtaatgc
    25021 cggcgtctcc ctgaaccaga ccggagatgg tggcgagcgc ggcagcctcc acatcaaaga
    25081 aagcccggcc ttgcaggttc atcccgccct gccagagggt gccgtctccg agcacaatcc
    25141 gggagtagcc tgtggagtca gccagatgca ggaatttcac atccccctcg gtggcgtcgt
    25201 gataagcgac tcccggcacg ccgtcggtga ggaccagccc cgcgctcatg cccacatccc
    25261 cttccgaggc caccgtctga ggcgtgcccc aagggaaact gccggtgaca ttgagagcgc
    25321 gcacatactt gaggtttttg gtccccggcg cggcgtcgta ataggcgatg gccggccggc
    25381 cctgggccga tgtgccgtcc gtgatgattt ccaggctcgg atacatgcca acatcaccgc
    25441 cgctgtccac cacaccggga gcgccccagg cgggatcaaa caccagagca ttaattgcgc
    25501 gtgtgaggat gttgccctgg gcgtcgcgca ggttgttggt gacaccgccg gcattcgtgg
    25561 cgcgcacata acgcagggat ttggcagtgg catcgtagta ggcgatggcc gggaagccgt
    25621 ccacgacgtt caaggaaagg tgactgccca catcgttcgc gctctcaacc accacagggg
    25681 cgctccagtc cgagccggcg ggattgttgg ccagcacata tttgagattg ccgttggtga
    25741 catcgtaata agccacggcc ggcgtgccgt tggcggcctc acccgtgatg gtcaccgtgt
    25801 tctgcgtggt gtttttggtg atgaggtcgc caccaatgtt caccatcttg agcgatgtcc
    25861 ccgcgccgac gttggccgag ctgtccagca ccagcgagac agcccaggag gcgccgttgg
    25921 cgtccagcgc gcgcgaatag cggaggcggg ttccgagcgt gtcgtagtag gcgatggcag
    25981 gcgcctcaag gccgttgttg gcctgctggc ggaccactgc cagggaagtg tagcaaccgc
    26041 ggtccaccgt ctggcggtcg aatgccgcgt ccgcaccgcc gtccgcgagt tcgagcctgg
    26101 cgaggaagtg gcaagtgctg ccgccgatgt tgctgaactc accgcccgcc agcagcttgc
    26161 cgctggcgat ctgaacgacg gccctgactt cctcattggc atccacattg aaggcggaat
    26221 ccacagggtc caccaatgac tcgggactgc cctccgagtg aagccgcgcg aggctcctgc
    26281 gagcgtggtc gccaaacgtc gtgaatgcac cgaccgcaag caccttgcca tccgcctgaa
    26341 ggacaagatc gcgaacttcc gcgttggcga taaccgagaa agactggtcc agggttccat
    26401 cggtgttcag cctcgccagc cgctcgcggc ggcggcctgc tccatcaatg gtcgtaaaga
    26461 ccccgccagt gagggccttg ccgttgggca ggatgaggat gcaggaaacc gtgttgttgt
    26521 agtttggatt gtagccggca tcaagggcac cggcagtggt gacacgggcg atgcggttgc
    26581 gggtggtgcc tccgatggtg gtgaaagccc cgcccagaat cagattgcca tccgcctgac
    26641 gcgccacgga gcgcacctcg ccattggcat tcgggttgaa gccggtgtca atcgtgccgt
    26701 tgctattgag cctggccacg cgattgcgcc ccgtgctgac gatgctggtg aacgatcccc
    26761 agacaacgat gcgcccatcc ggctcgatca aaagcccgcg gacgacgccg ttggcgttgg
    26821 gattgaagcc ggtgtcgagc gagccgtctg cgtttagacg ggcgatgcgg ttgcgcgcaa
    26881 cgtcgttgat ttcggtgaaa cttcccacca ccagaatctt gtcatccgac tgtatgacga
    26941 tgcccagcac ctcaccgttg gcggtggcag caaaggtgtt gtcaagactg ccgtccgtgt
    27001 tgactcgggc cagacgggtt ttttccacgc cgtccaattc ggcaaaggtg ccgccgatga
    27061 ccgccttgcc gtcggactgc acgccgatgg cgtttatggg ggctgaatcc acgatgacct
    27121 ggccgcccca gaacacaccg ctgatgtccg tggaacgaac aaacatgagc agcttgttgg
    27181 tggcgtcata atagctgatg gcgggattgc cattgaccac ggcgagtgac gggaactggc
    27241 ccacatcacc gcgggaggcg acagtgacgg gcggcagcca ggatgtgccg cgcggatcag
    27301 ccgcgcgcac gtacttcaag tcgccgttgg tggagtcgta ataagcgatg gcgggatgtc
    27361 cgttgaccac ggcggtggag gcatgggtgc cgacaagatc tccagactcc tccacagaac
    27421 cgcgctccag ccagtccgcg ccgcggctgt taaccaggcc gacccggaga tttttttcaa
    27481 tcgtgccgcc ttcgaccacc agttcaaggg ccgcgtcttt gcgcgtgttg gtggagttga
    27541 cgcccattct gacagtcgtt ccggtgccgg tcccgacgcc aaagacatcc tcaacgtccg
    27601 cggcaatcag gcgtccagcg gcgatttcga tgcctccgga atgcgaactg gagccgctca
    27661 aacgcagggt gccgtcccca aacttgatca gcctgccaag accggcaaaa ccacccgcca
    27721 gttcgccctc gacctccgca ggaccgtcgt tgacgcgcac cgcctggctg aagctgccca
    27781 gatccagtgt gttgagcacg atcgttttgt gggtggcgtc atgcgcgccc agcacgaatg
    27841 aggaaccatc cgggacaaat ccgttgttgc cgaaacgcag cgcctcggga actgcctgcc
    27901 cgccgatgtt gaccgtgcga tccgacccgt aggcggcaaa gccgccggcc ccctgaaaat
    27961 caatctgacc agcgccagtg ccgatgtctc gcgtgaaatc cccgtgcccc aggccaataa
    28021 cgccgccttt gacaactatg ccgcctgtgc cacccgtgtt gccgatgcct cccggcatgg
    28081 cgttggccga gttcaaacgc agcacgccgc cgtgaacgaa agtcctgccc gtgtaggtgt
    28141 tcacgccgga gaggatggtg gtgcccgtgc cggactgtac aaagctggtc acgtccagaa
    28201 atgtgtaggt tttgttgctc tgcgcgctga ccgcgttggc gctcatgtaa acgaggtcaa
    28261 agttcagatc cgtgcttaga accgtcgtgc catcagggat gccggggccg gaaatgatca
    28321 tgcccggctt gagcctgacc agggcggttt tacccagagg agagacgatc aaaatgttgc
    28381 tgcccgcact ggtcgtgcct gagttgacca tgctgagcac attgtccacg acgttcgccc
    28441 cgatggtgaa cgctgccacc gggttatgat tgtgaatcac caagtcgcct ccgtccaccg
    28501 cgcctgccgc tccggatatg tttccgccgc cgacgatggc cttctcaccg ccgcgcacgt
    28561 tcgccgcagc caggatggca ccggagagga gttgcagcgt cctgccggca gggacggtga
    28621 tggtgctgtc caccggggtg aaataacgca gcgtgttcag cgcgcggtca gccgtgagcg
    28681 tgccgaagta ggcgcggtcg cgtatgaagg tgtaagtggc gttgaagttg gaactggtgg
    28741 cgtttttact gagcacgacg cgcagcggat tggtcaggag agagactact tgggtgttcg
    28801 atgggatgcc gggaccgaag acgctcatgt ccgggatcag gttgccgaac tcgacttgca
    28861 gcaaatcagt gacgacgagc acattgctgc cggccgtgga ttccacgcca ttgtccaaag
    28921 agatttcgat gcgcccgccc tcggaggcat caatcgtggc cgagccttcc tgggtgcccc
    28981 agttgtcggc gttcatgaag aaggaaccga ggtcgtaaag cgaagccgcg tcggcggata
    29041 tcaccgcgcc attggacagg gcgacagtgg cgaaattgtt gacgccaggc tgggcaatgt
    29101 tcgtcgcgtc ctgatagacc gcccatggca tcaccctggc cctgtctgtc tcaggtatgt
    29161 tcagaagaat gttcgcgacc gaaccgccaa cttgaggctc ctcgacgaac aacacggtgc
    29221 cgccgacatt tctcagaacg gaggtgatct cctcagcccc catgaggctg atctgagtgg
    29281 tgcgggagat cgcgccgctg atcaggctgt tcgggttgaa gccggacaca cccaccgcgc
    29341 gcacctcgga cgcgcccgcg tccacataca tctgcccgcc caaagtcaac gtcatgttgc
    29401 cttcgcgcgc gccgcgcaat tccagggagc cgcctgcgag tctcaactcg ccgatgcccg
    29461 tctgaccaaa attgttctgc gcgaatgcgt tgggagccag attggtgtcc acaccctgga
    29521 caaccagcct gccctcaagc aaccgtatct ccgcaagacc ggcattgccg cgcatttcca
    29581 gcgtgccagg accggatttg gcaaagatgt cagcgccgga aatgttgcca gtgaagacgg
    29641 aggtggtgcg ctcgtggccg atgttgatct ccgccagttc ggacagggtg aagttgcggt
    29701 ttgaagacga ggtcattccc acatactgca aggtgccccc gttgaatgtg aggttggccg
    29761 ccgcgttggt tgatgcccca aggctgccgg ggacgcctcc atcggtgagc tgctgcgcgc
    29821 gcagtgttcc gttgccaata aaggtaatgc ctgaatagtt gctgtcggcg ttcaggacca
    29881 gggtgccgtc cccaagtttt tccactccga cggacggagt gaaggtgagc tgggacacga
    29941 tgccggtgta aacatggttc gccgtgtcca cggtgatgcc gtttccattc agcgtgccga
    30001 tggtaatgtc ggactcgttg acgatcgtct gcacccggta attggcgggg atgcccggac
    30061 cactgatcgg catgccgatg taaagatcgt gcgtggccag atttccctga gcgtccacca
    30121 cgccggtgat gcggttctgg gcgaactggc ggatggagcc gcgcatctgg atgctgccgc
    30181 caaaagtgta aacattgcgc gagttgtccc ctgtggtgat gtcggcactg acgatgaccg
    30241 tgcggttgtc aacgatctgc gcgatggtcg tgccacccgg catcccggtg cccgtgaccg
    30301 tcatgccgac agtgagctgg ctggtgtcac tgttgtcgct gaccccgatg agaccggtga
    30361 tgatgcggtt ctgtgtcgag gcggcgacgc tgggcgcggc caggcggccc gtcctgttga
    30421 acgactggcg ctgggtcagc ggcacgttga cgaccagatc gccgaggtcg ttgtactggt
    30481 ggaagacaaa gttttgcagg gcgccctcgt ttccggccat cagagtgccg ccgccggaaa
    30541 tgatggagtc attggcaccg acggtggggg acaccagcac ggcaccggtc tggagcaact
    30601 gccccgacgc gccggccagc aaatccaaag tcgcgggcag cccggtggca aaacgcagcg
    30661 tgtaagtgga ggcgccgtca ttgaccggaa tcggcgcgcc gcctcccagg acatcggtgt
    30721 tgaagttcaa gccccagtcc gtcgagtaat cgagaaaccc gtccaccggt ccgtcattga
    30781 agcttgtggc agtcgcggtc gcattgcgcg cccaggaata agtctggccg ccgttgtcat
    30841 ccacaccgtg ggcgagaagc tcgccgggag cggcataata aacgttggtg tcagcgctgg
    30901 catcgggcgc gctgctgaac accttgacga tgccaagggc gttcgcattg cccgcaagcg
    30961 cggccacgac ggcgtccgcg gagttgttgg cggccgtggt ggtgatggtg atccgccacg
    31021 gactttccac agttcccgat ccacccctgc cgaccgagat ggcgccattg ctgccgccgt
    31081 tatgagcgat gcgcacccgg atggtgccat tgcccttgga gccggagaaa ccggtgggcg
    31141 aggtctcggc ggggttcggg ttgggatccg cctggaaaat cagggaggcc gggccggggc
    31201 gcttcaccgc gccgaatgtt tccaccgtgt gagtccccgt gccaaaagtc acggtctgca
    31261 ccgcgggtcc gaaaggagtg gccgacacac ggaagctgcg ggaggtggcc tcggtcacat
    31321 aataggtcgt gtccgccgcc aaaccgtccg gcaattggcc agtggtggta aagcgcacag
    31381 ggactccggc ggccagatag tggaccggct caacatccgg caccttcaac atgcccgtgg
    31441 tctcatccac ggagaaagtg cggctgaccg tgccagggat caccaggaag gcctgaacgc
    31501 gcgcgtcgcg aacaaccgcc cagccgccga ggatgccgtt gatgttcggc atgtccacct
    31561 tggccaggcc agggccgtca aagtaaattg aagaccccgt ctggcgcgag atggtgttga
    31621 aacgaacgac acttgccccg ctgtcccgga atacccggct cgcgcccgcc tggaggctta
    31681 atgtgctgac aatttcctcg tgagtgcccc ccacaaggcg caggatgccg ccgcgtctgc
    31741 caccccccat gacaaaggtg gagttgtccg tcagtttgct gttgttgttg gagctgtaat
    31801 ccagcaccag ggtgcccgcc tgaagagtgt agctggagcg ggagtggtcc atgacatcgg
    31861 tcctcacggc gggattggcc gtcgccacgg tggggttgag gcgctcgcct ttcagcacga
    31921 cggtcccttc accaagctgg gtgatcgtgc gattgcccag gatattgccc gtgagagtca
    31981 ggctggcatc cagaccgatg gtccaactgc tgttggcctg ggtgtccacg ttgcccgccc
    32041 aggcggcctg ggcgcggatc ggcacaccga gtccgccgcc attgaagatg atggtctccg
    32101 gagccgtgta atccacattg ccgagatcaa gcctcccgcc catgacatag atgccctcgg
    32161 aggtgttgcc ggccggagag gcggaataat acacatggtt tcctgtcgtg gtgaaattga
    32221 ccggcgcgcc gcctggcgtg gtggagacct ggaaattcgg gccgctaact ccagacgcgg
    32281 cgttgacgac aaaataggtg atgttttcgg atattccgac tggcagcaga ggcgggtcaa
    32341 tggcggtggc cccgaacacc aggcgcgtgc caaacatcac gggcgtgcca ttgggcaggt
    32401 tgcccgtgaa cctgtcagtg tcgggattgg ccgtcgtggc ggccaccgag ctgatattcg
    32461 gcacataggt gacattggct ccatcagaca gcaggttcac cgcctgaccg ccgggagtcg
    32521 ttgacacttt gaatgtgccg gccaccgaat tggcctcgac cacataatag cgctgcccgc
    32581 cagtgatgcc tcctggcagc gttgtctggt tgaactgcgg aaaggtgagt tccgtgccgt
    32641 tgagcaggtt gccggtgaaa gtgtcggccg cgaaggaggc cgtggtggcg acgctggagg
    32701 ttccggccgc gtagttgctc tcaagcctaa gcacgccctc gagaactgaa gtctggccgc
    32761 tgtagtccgc ggccttgccg agcagccacc agccggaacc aattttttgc aagcctgtcg
    32821 gctccatcgc ggacttgtca cccagttgca gggcgagtgt gttcaaacct gtgttcacac
    32881 cgctgagggt cagcaggcgg gcgccgttgc cctcaaatcc gatcgtgtca gacgccacca
    32941 actcgtcaaa gaggatgaag ttcacaccca tggtgacact cgcggcgcga ttagcgccat
    33001 tcgccaccag cgtgccgccg ttcggacccg ctccaagagt gaatgacctg tccgtgcgct
    33061 gctgcacggt agggctgaaa gagagcgccc caccattgag aatcaaacgc gacgccgccc
    33121 cgttgccgcc cgtccccagg gatgacacca cgcctgaaaa aggcatgttt cgcacgtcaa
    33181 tggcaccgtc atttattcgc acgaccccgg aaaagccgct ttgcgtgccg gtgagcgtga
    33241 gccggcccgg tccgcttttg gtcagattca gcaaaccgcc gccaccgccg ttgtcgaact
    33301 ggccggcgaa agtctggttc tgctccaggt ccagggtcaa tgtcgctgag gaggcggacg
    33361 cgttgacaac acgggagagg gcgttgccgc tcaactgggt gaaacgatgg ttgatgccct
    33421 caagctgcac ctgtccgttg tttcccaagg caaccttgat cccgttggtg ccgagagcgg
    33481 ccggattcag cagcctcagg atgcctgaac ccatggtgac acttgggaaa tcctgcccgc
    33541 ccttgaaagt gttggcaccg gtgatgttga gcgtgccgcc aaggacacgg atgttttcag
    33601 tgaaatcatt atcgccagtg agattgaaag tcgagccttc catcagcagt ccgccgatcg
    33661 tgttgtttcc gtgaatgttg atcaccccct cgaaactgcc ctctgtttta atgcctgaaa
    33721 ggaaggaatt ggagcctcca ggggttccaa tggtgatgct gttgctctgg ccattgttgg
    33781 cattggctcg cacagccagc tcaaggggac cctcggcatg aagggggttg gtgatgttga
    33841 catgcacaat gggctcgccg gcgcccgtgg tgggggcgtt gttaggattg gcaaaaccca
    33901 cttcgaaacg ccctcccgcc gggccgatgg aaaaaccccg gtttccatcc gtgaagtcaa
    33961 acacattgtc cggcgcggga ttcggatgag tgatgtccgt gcgccgtcca aagacgattg
    34021 cagagccgcc ttcaaccctg aagtggtcct ctcttaacac ggaaggaata gttcccagcg
    34081 cgccgatgcc gcccaccatc aaggtaccgt tgctcaccac ggtcggcccg gagtaagtgt
    34141 tgccgaggtt gttcaagcgc agaaagcccg gccccgcctt gaccaggctc aaactgcgag
    34201 gatcgccaaa gtcacgtgcc tggttggtga agcctgggga gatgggctgg ctgacgacaa
    34261 attgcgtgga agtgttgatg acggtgatga ccgttccatc agggatgcct gcgccgatga
    34321 aggccatgcc cactgcaaga cccgtcgtgg tctgaccggc gccgacgata ttgaccgttg
    34381 tgctgttatt tgtaaccgtg acattgcggg aaatggtgta gtcacggatg gccgcgttga
    34441 aagtcagcgt gcgcgccgcg gccgcattgc cggtggtgcc gacgatggtg atcaactcgc
    34501 ggtcccccag cgcctccgtg cctgcggtga cgaatcccag agcctgggag ttgatggtgt
    34561 ggtcgccatg attggagagc agcccgccag tgacgatgtc aaggcgctgc tggttcaggg
    34621 tgagcgcccg gttctgggcg gcggcctcca agtccggctg gatgttcaag gagttgatgg
    34681 tgcgccgcgc ggtgagagtc tgcgcgacgc tcatcttgac attgttcgca gcgatccagc
    34741 ccacatcctg gttggagttg ttgtagtcag ccgccaacag ctgtgtcacg ccgtttgcgc
    34801 catatttcac ccactcattg ttggagtatg cccagccgcc gatgatgcca ttcgccagag
    34861 cgggcgcggc ggtgaacatg atccggtttc tcgtgtcagt acccagcgtg ttattctcga
    34921 ccgtggtcac accgccgacg agctgcttgg cctcgaagtc caccacaccg cccgggttgc
    34981 gctgcagacc cgcaaaagtc agggtggagg tgcgtccatt cgccgcccgg tagcctatca
    35041 cctggaggga tccgccgttc aagaccaggg tgcccgccgt ctcggagaag ctggccgcgc
    35101 tggcgtcatt gatgaactca aaaacaccgc cgttgctgat gatgggagtg gagtccggca
    35161 ggcggttggt gttgttcgaa aggttggtgt tggacaaaat caacgccgag ccgctggccc
    35221 cggcgtgaat ggccagggaa cgaccgctga tggtgatgtc cgcgggtgtg ccggtgattc
    35281 gtccatccgt gttgttggag aaactgaaag tcagcgccgt tgcattcgaa atggaggcat
    35341 tgttgctcaa agtcaccctg cgattgaccg cgtcaatggc ggtcaccgtg gtgccgcccg
    35401 gcacgccgcc tcccgtcacc ctcatgccca ccaccatgtt cgagctctcc gtgatggtca
    35461 caatgttgga attgctcgtg gtgcccgtgc tggtcttgcc gatggagaaa tcgctttcca
    35521 gtaccaaatt ggcctgcttg atgtggatgg agccggcgac attgacggcg gtgtccaccc
    35581 aggtgcccaa ctcctggtcg cccagaccga gcacagctcc cgaaatgttc atggtgctgc
    35641 ccgtgttgtt gaagcgtccg gaaaactcga ggtccccgcc gatggaaaca ttgccgcgca
    35701 gattgatgtt cctgttcaaa gcagcgtcac cgacgctgat caagtcaccc ccgatgctga
    35761 tgcctgcggc gctggcgttg agattgagcg tgccattgtt catggtgagc gagccgccga
    35821 tgttctgcgt gccgaagatt cccagcgtcc ccgctcccgt cttgttcacc gaccccgaac
    35881 cgcttatcga aagatactgg tccgggtaat ccttggttgg attcgagccg ggtatggcgg
    35941 gaaaactttt ccgcatgttg accgtgccag tgacgtcgaa gttgagctgc gaattgttcg
    36001 tgatgttgct gcgaaagttg gtggtgttga acagggtgag cgaacctccg gaaaccagcg
    36061 tgtcacccgt gtagatgatg ttgccgcccg cgagggtcag atttccggtt ccctgcttgg
    36121 tgatgcccag ccgcgcgcca ccgctcacag caccgttgga atccgcctgc tggaggttgt
    36181 tgccggtgtt gtcgcggaga aacccgttga cccgcgaggt caaactcccg gcaattgtca
    36241 ataccgccgc agttccaact gcctcgccct cgcggttttc aataacagcg cggtcaccca
    36301 catccagaat acggccgacc gtctcgttgc cacccatgag cttgaagtag gcgttgcgtc
    36361 caactccgct gatggagtca aaaatcagag atgccgaatc gctgatctgg tccagatttg
    36421 tccggccctg caagagttgc agcaccgcgt gaccattggt gccccgggtc gtgttgccga
    36481 tgatgatgtc gccacccgtg gcgttgccgg tcgcggcgcc aagctcgacc tgggcgcccg
    36541 tgcccgtgcc atttcgattc cacagccaga gattgacgtt gttccccgtg ttgttgcctg
    36601 tgagggccag cgtgccgttt cccacggatg tgagagatcc ggggcctgag attgtgccgg
    36661 agagggtgac gcggttgctg atgattcggg cgaccagatc gctttgcaaa gtcactgaag
    36721 tgttgatcac gtcagccccc ccctgaaatt tgttaaagta ggcgttgccg cctgaaaacg
    36781 agccgttgtc aaaaataaat ccaccacccg caccgctatt gaaggtgtaa acctgggtgc
    36841 ttgaaaggtc gcccagaatc atccgtccgt aggtgcggtt ggcatccagt gtcaaaatcc
    36901 ggttggccgt gatgtccgtg ttgaaagtca ctgtggagcc cactcctccg ggcgagggcg
    36961 gcggggcgat ggtcacggtg gggttcgccg tgtagccgga acctcccgtg ttgaccgtga
    37021 tgctggccac ggaaccgctt gcgttcaaat tggcggtggc tgtcgcgccg cttccagccc
    37081 ctcccgtgat tgttaccagc ggaggcgtga cgtagcccga ccccgcgttg gtgaccgtga
    37141 tgctgacaac cacctgggca gtgccgccgg tgccgcgggc ggcagtcgcc gtggcggtgc
    37201 tgccgatggt tctgtaaaat gccgccggat tggcaagcgt gccattggcg ttcgagagcc
    37261 aggagccgtc acgcctgaaa ctctgagcgg cagctccgcc aatgaggacg gtccacagga
    37321 ataaagtcag aaggcgcgct gccagggttc tcgaaaatgg ttgaatcctt ttcatagggg
    37381 tcgcgattgg atgatgacca gtccgccaga cgggcaggcg gggttgaaag agttctcagg
    37441 gaatgttcgc cagcgtcgcc atgttggcga tcaactgggt gtgaaccttg acagggacgc
    37501 ccgatgaagg gctgtcgaaa ttgaagtcag gcagaggagc ggccaccagc cggatcgtga
    37561 cccggcgcag gtggttgctg ggctgaagac tggcgttgtt ggtgggcaga cgcacatccg
    37621 cctgggggat ttccacgcgc gccacatagc tcagggcaaa acgatcctga gcgctggcaa
    37681 tgctgacaag ctcaagtccc tgatcatcaa aatagcgcac cttcttgcca taggtgtcaa
    37741 gaatggacca gtttgtgttc tgaagctccc cgacgatgtg ctcgatgatg cgcgcctcgg
    37801 ccatctcgtt ggccgttttc cgggacattt ccaacccatg cggcagcaac cccaggatgg
    37861 tgaccacccc cagggccatg attcccaccg ccagaacgac ctcgacaagg gagaatccgg
    37921 cggatttgtg aaacggcttt gtcacggatt gaaggggtgt tttcattgga gcgggagcct
    37981 gttggagttg gaatttcgga ccgatttttc gatattttgt tcagattaac ccgaatgccc
    38041 atgtaccgtc aatttaaata catggtaaat accgacccaa agtgctgctt ggggctttcg
    38101 gcccccaaaa acgatccgcc gggccttgtt ttggcccggc ggaaaagtgt ggaggttgga
    38161 ttgactcgga tacagccgga tttactccga gcggatgcca acacggtcga atgtcatgct
    38221 ggaaacagga ccgttgcctc ccgcaggata ccaaaccgct gagagatcgc caaaaacagc
    38281 gtttgccggc aggggtagcg cgtcccccag tgccacaccg ttcatggtgg ctaaccacgt
    38341 cccttgagcc aagtccaagg tgatttcaaa atgttgcagg gaatcagcca caagcagtgg
    38401 tgcggcggtc tgggtggtgc catccggctg cacgagttcc acgcttccat ccgcgct
//
LOCUS       DAOMYJ010000023        38271 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Prosthecobacter sp. isolate AMR_MDS_5629
            NODE_404_length_38271_cov_23.258661, whole genome shotgun sequence.
ACCESSION   DAOMYJ010000023 DAOMYJ010000000
VERSION     DAOMYJ010000023.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867850
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prosthecobacter sp. (wastewater metagenome)
  ORGANISM  Prosthecobacter sp.
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Verrucomicrobiaceae; Prosthecobacter.
REFERENCE   1  (bases 1 to 38271)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 38271)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.30
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:34:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,742
            CDSs (total)                      :: 4,684
            Genes (coding)                    :: 4,676
            CDSs (with protein)               :: 4,676
            Genes (RNA)                       :: 58
            tRNAs                             :: 55
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..38271
                     /organism="Prosthecobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_404_length_38271_cov_23.258661"
                     /isolate="AMR_MDS_5629"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1965333"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(146..1576)
                     /locus_tag="PLP58_04120"
     CDS             complement(146..1576)
                     /locus_tag="PLP58_04120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ07729.1"
                     /translation="MACSLKAQVVREEAPADAGAALIHHWPGPWQLMTEAKALALPDD
                     ASHSLDDFAIAFRVRVEKAPGGDHPLDMLTLEADAVLHLKVQISPSGHLSCSTPNEAY
                     CLFGDLPPGREVPVVVNVKRDARQALSGLWVDGVEMSSAVIKPWRAGVSGNVFYLGSQ
                     PLPLTVSDIRLYRRALTRSEILSLPLPGESRRLPAMGGRFELMQDEVLAVLGGSEAVA
                     VLEEGSLEALLMRAHAAQLPRVRSLAWEADTVFRQDRPLNFGSLPLQLRRCEATCVLM
                     MFGRQECLERGAEGVADFEAACGRVIAQVKEVTPRLVIVEAAPFEKKAPPLPDLTPKN
                     AALALYNEALRRLAEKHGAFFAGSGESWHHTRPEPLTRDGVNLTEAGAREVGLRVLAS
                     TRALEQSEPAALLAAVREKNRLWHHYWRPDNWAFLHGDRTAQPSSRDHLNPRLRWFPA
                     ELERYRALIAEKEQEIWKLAQPPKVP"
     gene            complement(1653..2141)
                     /locus_tag="PLP58_04125"
     CDS             complement(1653..2141)
                     /locus_tag="PLP58_04125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ07730.1"
                     /translation="MIDAVAGEFCEATMLSENSNINEAELLLLRNRIWISWLLWAASI
                     ISMIVVLAGFQDSWKYEVVIVGFFVFQTTRLLRSYRRPKIGKWELRRHWYGLTYLLVP
                     MTGLIVLAYWDSPLPLGGWVAFGMPVYLLHQVQIKDDIKILAVIPRLIQLLEQQKSMG
                     KA"
     gene            2246..3133
                     /locus_tag="PLP58_04130"
     CDS             2246..3133
                     /locus_tag="PLP58_04130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009959969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase 2 family protein"
                     /protein_id="HRJ07731.1"
                     /translation="MKLKLDLEDFRVPDGKPFRIAKAKTKIKNLYRDDAHYEQLFSEF
                     RVEIDDLQQKMYAQDRQGLLLIFQAMDAAGKDSTIKHVMSGVNTQGVEVHAFKRPTSA
                     ELDHDFMWRHLRVLPPRGRIGIFNRSYYEEVLVCRIHPEIVTKIQRLPAKTTKNLDKL
                     FADRLKDIANFEAYCDRNGIRIVKFFLHVSKAEQKKRFLARIDTPSKNWKFEEGDVKE
                     RGHWDEYMAAYQDTIRHTATDDCPWYVVPADDKKNMRLIVSAAVLHEMQGMKLKYPEL
                     PAEQKARLAAAREFLAAEK"
     gene            complement(3158..4765)
                     /locus_tag="PLP58_04135"
     CDS             complement(3158..4765)
                     /locus_tag="PLP58_04135"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HRJ07732.1"
                     /translation="MRIYMYLLIPLLCAVVSSCGTTSSMTPDALHDTSDKRGTVADKL
                     SAALSDAEKTLASGLRTVEQRQAYAAQVGRVTALWLSLAKDQKAVLHVDETYRLETRI
                     PANLLFDELIPAGTVKSRHLKRRITREGVGTPLIAHWKSSPERKAAEPLMTEAGYMTS
                     VTATLDFTRTSGGLRRATLALHDSRMTQEVILAGGKQPLAADLTAYGEHYLAMKDVRM
                     KGIKALLRSSEYMDKLGLFALERPDKNRIPLVLVHGLMSRPATWENVINELGDDPVLN
                     RHYQVYLFRYPSGVPVIYSSEKLREHLGTLHGILAREGAGMRGRNMVLIGHSMGGLVS
                     KAQVQDSGDRVWVNILGDTPAALGLSKEEHEALRRYLEFDPNPNISRIIFAATPHRGS
                     SMADGRLARIGRSLVKLPGQTFGSTFEVLESLASRDAYLSELLKRGMPTSVDNLSPGS
                     PYVRIANSLPFRPGVRLHSIIGNKEGRPLTDPECSDGVVPYSSSHLDNVESELIVRSG
                     HGVHEKPEAVEEMRRILRLHLKGLGLY"
     gene            complement(4904..6106)
                     /locus_tag="PLP58_04140"
     CDS             complement(4904..6106)
                     /locus_tag="PLP58_04140"
                     /inference="COORDINATES: protein motif:HMM:NF016830.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate porin"
                     /protein_id="HRJ07733.1"
                     /translation="MKPLPVCLLLAAFACLTTTAMAQEEVLTDEKRNEDGIAASIAIR
                     DQHWRNPREQRQQRRTAWMEALRLDIALSYDSLAIGAIGRDDGWGASSGDGTLNLRWQ
                     INADTSKAPLSLNLRMRHRHACSDLAPSDLRTETGALWGHVDGFTNAGFQVPEIFLED
                     KFFDQRLTLRYGQMTIDDLLDGHQLRSAKRSFMNQAFSSSPAVGFPGSDLGFVVRWEG
                     RAGWDVTLGASHLDSTNLSESAEWRFRGGALFKGTQIGCNFKGWNAEPARVQLLGWDS
                     DAMTEFDIQSGRGASLTFEQQLAGRAHSFLRYAWADGEAAVTDHFAAAGVAFDLRQSD
                     RLGIAFGAGSSSSNSSKREGVIELFYRRQIGKSLIITPDIQFVSGDGIGGQAEWIVIG
                     GVRAGFIF"
     gene            complement(6103..6756)
                     /locus_tag="PLP58_04145"
     CDS             complement(6103..6756)
                     /locus_tag="PLP58_04145"
                     /inference="COORDINATES: protein motif:HMM:NF016270.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF502 domain-containing protein"
                     /protein_id="HRJ07734.1"
                     /translation="MKRIAAFLKATTLGGLFMLLPLVVILILVGKVIVTTHSAAQSVM
                     DSLAGHDSVAAEFPMIYAVLIVVGLSFMLGLMMISRQGRTVGTWIERTLLYRVPGYAA
                     ARAIVGGLASMEREGVVKAGLLKTGEGDESFVFITEDHGNGKLTIFVPETPNPASGTV
                     HIVPKECVRPLHVRITSIATVLQQWGMGSAGLLAQHERHWDGRAETGEPPRSMLPQG"
     gene            complement(6899..9268)
                     /locus_tag="PLP58_04150"
     CDS             complement(6899..9268)
                     /locus_tag="PLP58_04150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ07735.1"
                     /translation="MKSRLLQTFTTALLTSLSVTATHGAPENILNPDAWPRVYEVGGA
                     EVAIYMPQILEWQEFRHLKGTSAIGVKLEGKEEATFGAVAIEADTITDFEHDTVRVGA
                     RQFTQFRFPELDAAGAAKAEELVRSVLKPETPLEMPLAAVIAVVNRDDATTADTAVSF
                     EPPPIFYSDSPAVLVTFIGEPKLEAVNEDDPTLMFAVNTNWDVIFNDGQYYLLTENQW
                     LVTQDVIKGPWKVAKTVPASFKKLPDDANWSEVKAKVELPSDSTPAPRVFAANRPSEI
                     IVTAGKPQLVPISGTSLMYVANTESDLFLHPAGKSYYLLTTGRWFSAASLDGPWASAM
                     DTLPEDFANIPENHPKAHVLASVPGTPEADEAVILASIPQTATVERASTTVQVAYEGE
                     PKFKAIPGSEGVQYATNTSYDVFLVNGIYYCCHQGVWFQASAAGGAWVVCDHVPSPIY
                     TIPPQSPKYNVTHVHVYNSTPTTVQVGATSGYSGAYVARGLLVFGLGMWLGHELADDD
                     HWHHHYYPSPYWYGYGCGAIYHPGRGYYRCGARYYGPYGGAGYGAAYNPATGIYSRGA
                     YAYGPRGAVAARTAYNPWTNTAAGRVAVQTPYGSWGRSAVVRDDEWVRAGHRSNAYGT
                     VGGVQTSRGGAAVGVNRKYGSDAFVAKTGSGDVYVGKDGNIYKKDENGWHSRSNGGWN
                     SAPNVPANNPPYPPRTSPPASRPERPTTLPAQPVTRPAQAPVKPAQPVTRPTTQPAAR
                     PSSGGFNRGSYQNRQSTPSQLNRDAYSRSRSSTVSRSTSSVSRSRGGRR"
     gene            complement(9299..9931)
                     /locus_tag="PLP58_04155"
     CDS             complement(9299..9931)
                     /locus_tag="PLP58_04155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ07736.1"
                     /translation="MTTPPLPAVRFHQALSCWLGCLLVAGGWGCATSLLADGKSKETI
                     ITKGEPGGLLVETTELSATVVKVNKSTREVTMVDPQGRKSTYRAGPEVRNFDQIEPGD
                     RVRAALTEQYLSFVRKPGSEMPASAEQVRQKVVVAPKGAKPSVLIVNTSEYTGRVHSI
                     DAKNRTATIVFADGRRQTFKARPDVDMTRHAVGAEVVFRTTESVAVSITK"
     gene            complement(9951..10706)
                     /locus_tag="PLP58_04160"
     CDS             complement(9951..10706)
                     /locus_tag="PLP58_04160"
                     /inference="COORDINATES: protein motif:HMM:NF023197.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3313 family protein"
                     /protein_id="HRJ07737.1"
                     /translation="MKPTCLIPCLSVVLFSAALTSCKLLETKEAPDSGFNPPTTATKN
                     TRAAFLQEVWVSPAYQGTAVSQKFKSVYFAPVNTQHMAKQSWWQAQTPLRQNDLAQDT
                     RKIATRLRHEFMTAVANHPAHKMKLALSPGPDTLVIELALVELVPSKAFWNAASTAAG
                     FAVPGAGYLSMAGRGSIAIEGRARNGADNSIIATFKDRRTDKVAPVNLGKYTWYHGAE
                     QNISEWAAEFASFLNTKPDETIKRTSRVTLKPW"
     gene            complement(10891..11664)
                     /locus_tag="PLP58_04165"
     CDS             complement(10891..11664)
                     /locus_tag="PLP58_04165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ07738.1"
                     /translation="MKLHHLMAVIALTASAHAGTPAPAATLEPIPAASDWEFRLGLPG
                     WISGIEGDVGVFGAVSAIDVPFDDIVSNLDMVAAANFEARKGRWGLLTGLAYMELSGA
                     AGTPGPLINNVTLEMRQLMIDAAVSYALVDGASCRVELLGGARYNHIDLGLNITSPLP
                     VLNRDISGDKGWVDPYVGFLTRAKLAEPLTLVLKGDIGGFGVSSDITWQAYAGLEYQI
                     SKHMYAGLGYRALGVDYTDGGFTYDVITSGVQIELGLKF"
     gene            complement(11814..14852)
                     /locus_tag="PLP58_04170"
     CDS             complement(11814..14852)
                     /locus_tag="PLP58_04170"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component regulator propeller
                     domain-containing protein"
                     /protein_id="HRJ07739.1"
                     /translation="MITTQTTADGLVTNDLLCLARDGADGLWIGTDQGLQHLENNKFK
                     QVGESEGVRGRVGAMAMTPSDGLWIRVQNLGLVHVHQERCEVVEPLPSSRGLSASSLL
                     ADRDGALWVGMTPHVMLRRKDGKWTEFNQANGVPYLAPFCMAAGNSGEVWLGGYDDGL
                     HVMKDGVVRPVPGTDKETRSLFVMPDGTVWVGTDAKGLFRLTRGKVRSYVLADESMGG
                     GVNSLVEEPDGTFWVSTYGTGLYHGSLGHLNGVMNGDQPVGGNLMRTALRMRDGTIFF
                     AGDQILVRKAPNSEVLETTVISDIPRALCEGGDGSLFMGTNAGELKRLEGRVFHTVEK
                     GKFPHSIQGLVRGRNDSMWLATRAGLFHWSAGQVKRWTTQEGLPTDILTCLHLDADDT
                     LWIGTGGGGLAWLENGRFYSVDARQGLGGNVMSQLLEDEQGHLWLGGYHGITRVKKDE
                     LKAVATGKARTLHPLLLSKSHGMMSFECSGGYAPAGLRSASGVLHFAAGRSIIAINPR
                     DWNVETEPPKVMIEGVRVGGKPVTTRGGSLTLPPGTLDLEIEYTAFHFAAPEGVHFRH
                     RLAEDRHWEEDMGVRSIRYSDLPAGDYVFEVTAANPDLRWQPATARLQITMQPHLWET
                     GVFRLLLVGLLVASGSQWARWRLRSRRRQEIEELERLRERQRADEKFRLALESSPNAI
                     VLAKEDGRIVLVNEQTEKWFGHTREELAGREMCTLIPDWPHAASETKAMNTGRERFGR
                     RKDGTLFPVEIRLSQFETEAGPLTLAAIVDISQRRQAEQEVARLSRISILGEFAGAIA
                     HELNQPLAAMLSNSQVGSSMMDEPKPDRTEMKAILDDITADAKRAGGIIHGMRAMFKK
                     EGAVPDEAVDVNEAVKQLLDLLHSEIMARKVKVDLRLGGPLPCVPAGRVELQQVLMNL
                     LLNALDALRGAAVAQPRITLSTALEADRVLISVQDNGPGIPPQVLDRLFEAFTTSKPT
                     GLGLGLAISQSIIKRFGGELCGENHAEGGAVFRIKLPVAG"
     gene            14865..14999
                     /locus_tag="PLP58_04175"
     CDS             14865..14999
                     /locus_tag="PLP58_04175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ07740.1"
                     /translation="MTEPASHRRDPEPAFAIVHQRPDVQSFQSFCQPEMDETNPVKPR
                     "
     gene            complement(15253..16266)
                     /locus_tag="PLP58_04180"
     CDS             complement(15253..16266)
                     /locus_tag="PLP58_04180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009960755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4105 domain-containing protein"
                     /protein_id="HRJ07741.1"
                     /translation="MKHAVRVGKKLGRCLVWLLLLLATAWAFGALYFDFPVFHDVVAG
                     LFVVGVMTALFLLRGFWKQTACAFALCGLVLAWWLTLSPRADRDWQSDVAELPWAEIQ
                     GDEVTLHNVRHFDYRTATDFTPRWETRTLRVSQITGVDLFINYWGSPWMAHPIVSFQF
                     ADAPPLCFSIETRKEVGEIYSAIGGFYRQFELYVVAADERDVVRLRTSIRHGEESYLY
                     RTRLTPEKARERFMEYLATLNALREQPRWYNAATTNCTTAIRGQHPAGKRPPWDWRIL
                     VNGKADSLLYERGYIETGGLPFAALREKALINPAARAADQSTDFSSLIRVGRPGFDGT
                     RSE"
     gene            complement(16348..17835)
                     /locus_tag="PLP58_04185"
     CDS             complement(16348..17835)
                     /locus_tag="PLP58_04185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009958050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arylsulfatase"
                     /protein_id="HRJ07742.1"
                     /translation="MKHIGKLLVLALGMALTAVTETEAAAKPNILHIVADDLGWKDVG
                     FNGATDIKTPNLDALASGGAKFSQFYTQSMCTPTRAALMTGRYPFRYGLQTIVIPGPA
                     GYGLDTTEHLLPQCLKDAGYTTAIIGKWHLGHADIKYWPKQRGFDYQYGAMIGELDYY
                     THSDAGVLDWFRNNQPVKEEGYTTQLIGNDAVKYLGEQSADKPFYLYLTFNAPHTPYQ
                     APQEYIDRFKNIKEPTRRTYAGMVSCLDDEIGRVISALDKKGLRENTLIIFHSDNGGT
                     KNAMFAGQMTDLSKVVLPCDNGPYRDGKGSLFEGGSRVAACANWPGHIKPQTVDGIIH
                     AVDLYPTFAALAGASTAKCKPLDGVNVWDTIAKGKESPRTEVVYNVEPFRGAVRQGDW
                     KMIWRTLIPTSVDLYNLADDPYEQNNVASKHPLKIHAMQERLNALGKESAKPLALMYV
                     AKVGLAHGKPNMASPDGKQPAAEPVHGHSITDEGVGQKDIHAEQK"
     gene            complement(17879..18469)
                     /locus_tag="PLP58_04190"
     CDS             complement(17879..18469)
                     /locus_tag="PLP58_04190"
                     /inference="COORDINATES: protein motif:HMM:NF016269.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YSC84-related protein"
                     /protein_id="HRJ07743.1"
                     /translation="MKHTIQTLAVTTLLAVSSLSLSSCANGPLTQANAANSSKAQIAS
                     SSRAALHQLYAANPKARWLGSQAKAVLVFPSIVKGGFMVGAMGGNGSLVWRNGAIRDF
                     YQTVGLSYGLQAGVQTYGYALFLMDDSALRSLNSSQGWEVGSSPSLVILDQGMAASLS
                     TNTIQKSIYAIFFNQQGLMGGLGLQGSKITRIYPAP"
     gene            complement(18501..18893)
                     /locus_tag="PLP58_04195"
     CDS             complement(18501..18893)
                     /locus_tag="PLP58_04195"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HRJ07744.1"
                     /translation="MPHRRPTIFIIDDDTSVRRALGRVMTSAGLDHQAYDCAEDFLNE
                     APQTSVGCIVADMTLPGLSGLDLKLALNAAHSPLPLIFLTAHDTTESRAAAQAAGADG
                     YFRKPVDTQALLDAVRWSLNEQNTQEHA"
     gene            complement(18972..19607)
                     /locus_tag="PLP58_04200"
     CDS             complement(18972..19607)
                     /locus_tag="PLP58_04200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009962205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HRJ07745.1"
                     /translation="MSLTPPTVFIVDDDASICRSLARLVRQAGLDGRTYPSAEAFLEA
                     HPAPPSRPACLVVDLQMPGLSGLELQQRLLESHSSCPVVFISGNGDIPSTVRAMRQGA
                     VTFLTKPFDNDDLLRAIGEGLEKHRATLAAGQQVSSIRERMKTLTERELEVMSWVITG
                     ALNKQVAAELNVVEKTVKVHRARVLEKMQAQSVAELVRLCALVGFAAAEPS"
     gene            complement(19698..21125)
                     /locus_tag="PLP58_04205"
     CDS             complement(19698..21125)
                     /locus_tag="PLP58_04205"
                     /inference="COORDINATES: protein motif:HMM:NF039990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DcaP family trimeric outer membrane transporter"
                     /protein_id="HRJ07746.1"
                     /translation="MALLATAFGPQAHAEDEADELARLRATVMRMEKAMQEMQGQIAE
                     LQKQKKSPAPAVSLSAANKPSLTPAEAVKTKPTPDAAKLSDSRSMMRHRETVTGDNLS
                     APRPGNAPMDPTYQGFMHIPDTTTWIKLGGYAKVDSIVDSTKMGNPNKFSTGGIPVEG
                     EANFGKGREYALHAKQSRFNVELRTPSPFGSVRFFYENDFFANSASPDMEYNLRHFYG
                     QVANVTVGQTWTTFFDPDTIPDTLDFAGPGSQSILRQPQVRYTFSPIADHMHVALAVE
                     QPKSDIGALPAGATTRTSMPDITGHWRLEGAAGHVQVGGLARSLAWEGAAGAEDSVFA
                     WGTNISGTLNLRGNDSLVASLTYGDGMGRYIQDLPAGSGAVVDASGRLNTLSGWGGHV
                     GYRHFWADRWRSEFSIGTVDMETADEQGPKAYERTLYSEVNLIWAPSKRFYVGLGYLY
                     GSKTTRDGSEGDAHRIQMSMQFKLF"
     gene            complement(21188..22015)
                     /locus_tag="PLP58_04210"
     CDS             complement(21188..22015)
                     /locus_tag="PLP58_04210"
                     /inference="COORDINATES: protein motif:HMM:NF012420.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase family protein"
                     /protein_id="HRJ07747.1"
                     /translation="MKTYPSLLFALVLFSILVACKDRSNPGTEHGTARPAHWGYEGKD
                     GPEYWPALNPAYAACGQGKSQSPINITQTADGDARAWKLDYKKSSLKIAHHEHVTDIV
                     DNGHTIQVSVDGGSTLTTDRGVYSLKQFHFHTPSEHTVDGSYFPMEMHFVHQSADGKF
                     AVVAALIQEGSENENLGKIIPHLPKAKGDSAHLPEVELELSFHLPATTASYTYMGSLT
                     TPPCTEEVEWLVFRDPISASKAQIEAFASRLSPSNRPTQPLNGRIIGSRTMVNKVRP"
     gene            complement(22084..23907)
                     /locus_tag="PLP58_04215"
     CDS             complement(22084..23907)
                     /locus_tag="PLP58_04215"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HRJ07748.1"
                     /translation="MSLIVILWSMAAAASLSLGVIHGMVWLSNRKVRAELWFAVAAGC
                     LTAYPFFDLQMMMAETPEEFGRAARWALTPISLVICAFLMLVHCYFGTGRRWLLVAAL
                     VSRGTALLVNLLCPHGLFFERVTDIRKVSFLGETVSAPIGEMGRWLWVEHLAAVIWTG
                     YLLDASWRLWRTGGREGRRRVVMIGGGIIFILTAGLAQASLVVAQVIALPVAVTLTFL
                     ILLTVMGYELSRDVLRAAHLASSLKDSEQRLEMAASAAQLAPWEWDIQTNVIWTTNEG
                     RRIYGIPPDEVVDFPRFISTVHPDDREALEAEVRATLDESTPFAAEYRVVLPDGSERW
                     IGAAGRLERTPQGRPLLLRGVSMDITQRKHSEQELAQKRQDVTHLSRVSMLGELAGTI
                     AHELNQPLAAILSNSQVGHSMIRSGKPDMEEMAAILEDIVADAKRAGGIIHGMRAMFK
                     KETAMQVQPVDLNEAVQQTCSLLHSEIVVRKAKVKLNLAESLPQVMAGRVEIQQVLIN
                     LILNSLDAMKSAGAASSEVGITTELRAGSVVIRVVDNGPGIAPEIQARLFEAFATSKP
                     GGLGLGLAISQGIMKSFEGELTGGNHPEGGAVFEMTLPVPE"
     gene            complement(24017..25903)
                     /locus_tag="PLP58_04220"
     CDS             complement(24017..25903)
                     /locus_tag="PLP58_04220"
                     /inference="COORDINATES: protein motif:HMM:NF016800.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HRJ07749.1"
                     /translation="MQSLSVILMCLGLVLSWVLATETRPLFFWPGALLLGFAGAVAAL
                     RWRLRVMFAPSDACLLAASLLTVHVMVRAWLSPVPAWALEDMVIAASCWVVYLLVVTA
                     CSHPQRRAWLLVLLLAAAAGNLAAGFVHFSGRWDFHVVPDFVRAAGAGRIGGFFANPN
                     HLGAFFAMALFLSAGWLCYGRGGALLKALAGFLIVAMAAGCALTLSRGALISLAAGGA
                     VFAGLSLLMVWRARPQVFWPLLGGGLLAVMAAGVVFWKVNEEQMLTRELRSPVSGDVR
                     LAVWKSALAQHEEAPWLGAGARMFYEGSIRLRAPELPVTAADPLFAHNEYLQMLADYG
                     WAGLALALLMLAAHAWSGLSFLRWFIRHRYPVTGRLAGNSLGLAVGSLAALAATAAHA
                     AVEFHFHVPATALTGALLLGILANPGLDDPKRAPVRLPGARFAAKALLAVFSFGLLAC
                     VWTQARGDYFLARAAVAQVRGDRAERLQMLARAAAASPGNPEVHYQRGLALLEKVDAD
                     KRDAAQADLEAAAAALAEAVALNPARYLHHLALADACDALGRHDEAFGHVRSALALAP
                     VHEEPRLALGIHWHRLGDFARAERAYLWASEAGAWNDEETARWPDNYRLLLQHAALMR
                     RQSP"
     gene            complement(25903..27891)
                     /locus_tag="PLP58_04225"
     CDS             complement(25903..27891)
                     /locus_tag="PLP58_04225"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase"
                     /protein_id="HRJ07750.1"
                     /translation="MLLRGTLILLLCAPAFQAASAAAPKSKSAYGKPNVLFIIADDLR
                     DYTGWLGGHPQARTPHMDRLASMGMRFANAHCSYALCNPSRTSLMTGMAPASSGVFGN
                     EQDWRRSVQITGKTTLPEHLRAMGFLTAAAGKIFHANHGGPEARLAGWHGGRRGFEQD
                     AAWERRFPAPGVQIPEMQVRIGQDFNGLGIWHWDWGALDLPDELTDDGAVTQWAGGFL
                     KQKQRKPFFLAVGLYKPHSPWYVPRKYFDPHPLDETVLPEVRADDLDDVPAFAKGHEK
                     QGGHHALILARDKWREAVQAYLANVAFCDARLGELLAALESGPNARNTIVIFTSDHGW
                     YLGEKQMWHKGKLWEEATHVPLTVHAPGLTKPDSVSGEAVSLLDLYPTVCDLLKVAKP
                     KHLDGESLLPLLKDPSAKRARPAITAMGGADKASYAARDRRWRYIRYSDGGEELYDHD
                     NDPNEWTNLASLPEHEAVKKELAAHFPKVFQSARRPAGEIVKGPSQDGSEHLELQIGD
                     ELSAGESPKLAGRGVFVDAAFDFNPEVDMDSTLVGHGDAKLGWVLHLVDSAPVFTVFI
                     NGKAGSATISPLKAGPCQLRATLDYDGLISLAEPGRSEVLERASFTDGFPGNPGKGIA
                     AGQSFGILNVKDYPNSTPFDGAVRRLRVTFLPPVKGKP"
     gene            complement(27945..29408)
                     /locus_tag="PLP58_04230"
     CDS             complement(27945..29408)
                     /locus_tag="PLP58_04230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ07751.1"
                     /translation="MNEEIEQLKDTLASMRTELDELKSRLARISRYVTVDNEDDDGVE
                     RVYMECTDFTLRPAHDRRQIAAHVGAGQQGVYFDLHYPGMEHNMHTAIRLSVDEEGVP
                     HVQVFGRDWKTRLELMVQNDHGLAAVLGAEGAPGAVMRALPGGGSVAVLQPDGRTRGV
                     LFHQDGGEEGQGAQEKTSSTDLLFATAQGSTVLKLHTDGTSSFMAAGTPAQPDGAVLG
                     MGPDGGSLMLHSKENKNSISMMCGEQISCLKMHPGKVPDGNDGLSLLAGDFGSSVELH
                     DEKGAQRVGISALGGGAGLVFLKDDEDEALALRHAPGSHSSLSMRGASKAEGVLLFAN
                     DETAFVKVTSPDDETCHISASVAKGSPSLMVTDGSRPLVMAGRTEHGGVICAYGLKEA
                     EGGIASLSGGPLGGELAICSNDGTRLMTLHSTDHGGRMLINNDLGMQRVTLAAYDEAG
                     SMVLNNTGSNGLIATVTPEGGRLTLLDPEGKMLAQLP"
     gene            29545..30894
                     /locus_tag="PLP58_04235"
     CDS             29545..30894
                     /locus_tag="PLP58_04235"
                     /inference="COORDINATES: protein motif:HMM:NF012264.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HRJ07752.1"
                     /translation="MTRPCLHVFLAALFYAGAPETRAHPLMAEPVNHPLVFTFDQFYL
                     TVDPDEHLAGGGHLLLAELNCSACHAAPDAWRPWLAPKPGPRLDGVGSRMDADMLWLL
                     IRSPQHLKKGTQMPGLFSGAEGDEEKVEAVVEFLSASTWDAPQMPAGDAMRGRNLYHE
                     TGCVACHEPASDHRPANAPEDAELDRPGNASVPVVLADVWHQDALAAFLHQPLAFRPA
                     GRMPDMLLTSQEAADIAAYLHLGRTQPGNALRAALQIPPQGIERGRQVFHEMRCAACH
                     EAPGSSPVTAPSSHPMRALRLDQGCLAERQTSGIPRYDLNDLQKRALRLALISLQARA
                     KPDHLAGPAQQTDWQMTRLNCYACHDRGYKGGPEDPRALHFTGTGLAIGQPGGSAHLP
                     PSLDQAGARLGREGLEKILLGPRAPASSHTRMPLFGAPQVRPLVDWLLETDKGMPAR"
     gene            30953..31303
                     /locus_tag="PLP58_04240"
     CDS             30953..31303
                     /locus_tag="PLP58_04240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ07753.1"
                     /translation="MSPAFYHIAHVVGLIFIFTGFGALLADTASRAAMKWHGIGLVIS
                     LVSGFGLVAKLQLPYTSTWLLVKMGLWLVLGFLPVLARRKVLPAGVVVLIAIVIGGGL
                     AFLGYPTGGRVMLP"
     gene            complement(31314..32516)
                     /locus_tag="PLP58_04245"
     CDS             complement(31314..32516)
                     /locus_tag="PLP58_04245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009960799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HRJ07754.1"
                     /translation="MMENASSLSDDLTRRAPYDAADHLSEVPPVEAATALIEVNPMLA
                     QQILSEMDERKRCEVLAAAPVEKARQWMRNQEYPEDSVGWLMEPPVAVFRPQMTVRET
                     IEALRVLTKKAFITYGYVTDGQNRLIGVLVMRDLMLGDPDKPLSEVMWTRLFTLSPEM
                     SLTDAMKASVNRHYPVYPVCDAGGHLIGLVRGQSLFEARAIEISAQAGTMMGVEKEER
                     LTTPLLRSLKLRHPWLQVNLLTAFAAGAVVGAFQDTLDQVLILAVFLPVMAGQSGNTG
                     CQALAVALRGLTLGEIGSGQERRLVIKEGLLGLLNGALVGLTAGLGMTGYALMQGHEK
                     AVLLGIVVFISMIASCIVSGLCGAMIPLGLRRLGADPATASSIFLTTATDIVSMGFFL
                     GLARWLVV"
     gene            complement(32591..33046)
                     /locus_tag="PLP58_04250"
     CDS             complement(32591..33046)
                     /locus_tag="PLP58_04250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009963788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HRJ07755.1"
                     /translation="MIRRIMRSTPDHSAALDEVRAIYAELEKRPLDRQCVARAQCCHF
                     RLTGKTPMLTLGEALLAARGVRAAGRKKLTPHPDGACPMLGRDGRCAIYQHRPFGCRT
                     HFCQAAGGVYPRRHIADLIQRLEALDEKLGGDGPRALEGAVSDALARIG"
     gene            complement(33043..33594)
                     /locus_tag="PLP58_04255"
     CDS             complement(33043..33594)
                     /locus_tag="PLP58_04255"
                     /inference="COORDINATES: protein motif:HMM:NF014606.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SLBB domain-containing protein"
                     /protein_id="HRJ07756.1"
                     /translation="MNSRLLLLLVFCAVSQRVHAQGELPLKSGDRITISIGAIPDNEV
                     VQINKVYTISDNGTINLLHIGEVRASGLKPSALQRSIEQIYVSREIYARPNVLVSIDT
                     DQTARQVYVTGVNKPGPVPYRPGMTLSQAIQFAGGPTPFASMKRVRLVRGARVTEHNL
                     SRDIGNPAVDVTLQPEDQINLPE"
     gene            complement(33603..35774)
                     /locus_tag="PLP58_04260"
     CDS             complement(33603..35774)
                     /locus_tag="PLP58_04260"
                     /EC_number="2.7.10.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045226 - extracellular polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis tyrosine autokinase"
                     /protein_id="HRJ07757.1"
                     /translation="MSEASPDLQNHALDSWRVIRGRAGLIILSFLLVFGSAAMLTYIM
                     PRKYRGRVEMKIERLQDKVQVFERSSDNFMANSDVVVKNEFESITKPETLYPVVDKLD
                     LQLKWELPTRQAALGKLRSNLDTQSSIRSDFVIIEFFDKDPALAATIANAVADSYMTR
                     RVEVESKQKESALEMLNKQITEQEQLRAQAQLKMQEAKTKAGIVGEWFSAGPNSTVPG
                     AELRTTGDSMAVTREQAIFQADLDVETLSAEIGQLQTLEGDELVARAGGLKLDNANVA
                     QMTGELQRLEVAREGLVNQGRGRRHPEVLANDSQITKYKELIAAAAKDHLAGLQNRLE
                     SARKRKEALEASNQDAKSELLGQQSAFNEYKTAKDDVTDIEAILRTLKTRYFETKAGL
                     EIFKSPATIYAKAEPEGRPAKPNVALNLALGGILGLMFGFGLAFALEFMDTSIKSLDD
                     VERFLGVPVLAVVPKNVGILHRATGFNPDAEAYRILRTNIEFNRKNRDANCITVTSGG
                     AGEGKSTTLCNLAVVCAQGGYSVLLIDADLRRPSMHTHFDAGNTTGLTNFLTTNVRME
                     DVVVRTAVENLYFLPSGLLPADSAGILNSQRMSDLIEDAKSRFDIVLIDSPPILGVSD
                     ASVLANEADLTMIVVQHRRLPRQTLMRVKQTVENVGGTVLGVVLNNVDLRADSQYQYY
                     TNYYTYYSPDNRPAAEGGKPKKRRKAAGETAPARTPGGDLF"
     gene            complement(35886..37004)
                     /locus_tag="PLP58_04265"
     CDS             complement(35886..37004)
                     /locus_tag="PLP58_04265"
                     /inference="COORDINATES: protein motif:HMM:NF021589.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF481 domain-containing protein"
                     /protein_id="HRJ07758.1"
                     /translation="MRNFSGDFTEDQPLTWSITTRGGYDNLKYDVPSAFLPDFESWYI
                     QGGVGMTYTEADPVTPWSMDLDLGATHYLDDMPRYDDTFYNARVAFNLAHKLSERLKI
                     SNNFYLTYEAEPNLAFGGSTTLFNGQYLYGYNNFNVSYAWSQRFSTTTSYTVDGITYD
                     DNLVSSFEDRLSHLVAQQFSYAISQRTTLNAEYRYRHTQFRAAGRPDFQSHFALAGLD
                     HAWSERTTGSFRAGAEFFRSYGGWKSAPYGEIALSHQVARKTTARWFGVAGYDGAELG
                     AYDSRYALRTGVNFHHQVTKRFGVQAGASYAYSEFDGDGAITDVTEHSLLLSTGLSYQ
                     ILENLSLDTRYSYSLLDSDDAFRSFDRHNVSVGVTASF"
     gene            complement(37131..37703)
                     /locus_tag="PLP58_04270"
     CDS             complement(37131..37703)
                     /locus_tag="PLP58_04270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ07759.1"
                     /translation="MSALSAFRFFGLTFTAALLGTMPTHGQNSSCANISSDVTAAVSN
                     DPSKVLMIVEDALVINESCAGEIIKAAIIASKADAALVNQIVQTGISVAPKLASVITD
                     SATAVSPGTAIAAQFVAAPQDLNTSAKNPAKNPLPVAPEPVFEPVVPASIRGVYLIQP
                     PASGFIPRDPRCRKDCDDPLSPSQNQPGFN"
     gene            complement(37740..37813)
                     /locus_tag="PLP58_04275"
     tRNA            complement(37740..37813)
                     /locus_tag="PLP58_04275"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(37779..37781),aa:Gly,seq:tcc)
     gene            complement(37908..>38271)
                     /locus_tag="PLP58_04280"
     CDS             complement(37908..>38271)
                     /locus_tag="PLP58_04280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ07760.1"
                     /translation="GLVRCRFGILDFLMKFRGWSFYHRVSRILLVFEATFRPNSKPDA
                     WTMPVEQALILKLDIFVEPNFTLTVNKISEKSCALNHGLTQLRVPQDLKNQLLHGPRR
                     WLDIRILLKDRCGTQSGC"
BASE COUNT         7263 a        11760 c        11961 g         7287 t
ORIGIN      
        1 gcgcgatctg acccctcaac ccctaacaaa tctcatttca aaagcggcgt tcgggttgaa
       61 cgcttcagca tcgtcgaaga cttgacggac ttctatggtc gctcacttgt ggcagtgaac
      121 cgttccggcc tgtgccggag tgggttcagg ggactttggg gggctgggcg agtttccata
      181 tttcctgctc tttttcggcg atgagggcgc ggtagcgctc cagttcggcg gggaaccagc
      241 ggagccgggg gttcaggtga tcccggctgc tgggctgggc ggtgcggtcg ccgtggagga
      301 aggcccagtt gtccggccgc cagtagtggt gccagaggcg gtttttctcc cgcacggcgg
      361 cgagcagggc ggcgggctcg ctttgctcca gggcgcgggt ggaggccagc acgcgcagcc
      421 ccacctcccg cgcgcccgct tcggtgaggt tcacgccgtc gcgggtcagc ggttccgggc
      481 gggtgtggtg ccagctttcc ccgctgccgg cgaaaaacgc gccgtgcttc tccgccagcc
      541 gccgcagggc ctcgttgtaa agcgccagcg cggcgttttt cggcgtcagg tccggcaggg
      601 gcggggcttt cttttcaaaa ggggcggcct cgacgatcac caggcgcggt gtcacctctt
      661 tcacctgcgc gatgacgcgc ccgcacgccg cctcgaagtc cgccacaccc tccgcgccgc
      721 gctccaggca ctcctgacgg ccgaacatca tcagcacgca ggtggcctcg cagcggcgca
      781 gttgcagcgg caggctgccg aagttcagcg gccggtcctg cctgaaaacg gtgtccgcct
      841 cccaggccag gctgcgcacg cgcgggagct gcgcggcatg agcgcgcatg agcagcgcct
      901 ccagggagcc ctcctccagc accgccaccg cctcgctgcc accgagcacg gcgaggactt
      961 cgtcctgcat cagctcgaac cgcccgccca ttgccggcag ccgccgggat tccccaggca
     1021 gaggcagact gagaatctcc gaccgtgtca aagcccggcg gtaaagccgg atgtcggaaa
     1081 ccgtcagcgg caggggctgc gatcccaaat aaaacacgtt cccggagacg cccgcccgcc
     1141 acggtttgat cacagcggag gacatctcga ccccgtccac ccacaggcct gacaaagcct
     1201 gccgcgcatc ccgcttcaca ttgaccacca ccggcacctc gcgccccggc ggcaggtcgc
     1261 cgaagagaca ataagcctcg tttggcgtgg agcaggacag atgcccggaa ggcgagattt
     1321 gcaccttcag atgcagcacc gcatcggcct ccagagtgag catgtccagt ggatgatccc
     1381 ccccgggcgc tttctccacc ctgacgcgga acgcaatggc gaagtcatcg agggaatggg
     1441 atgcatcgtc cggcagggcc agcgctttcg cctccgtcat caactgccag ggtccgggcc
     1501 agtgatggat caaagccgcc ccggcatccg caggagcctc ctcgcgtacc acctgggctt
     1561 taagcgagca ggccatgcca agactcaaaa agaaaagtcg aaccgacatg gtgcaaaaac
     1621 atgcgccaac ccgatgatct tgcccatgaa aatcaagcct tccccatgct tttctgttgc
     1681 tctagtaatt ggataagtct tggtatgacg gcgagaatct tgatgtcatc cttgatttgc
     1741 acttgatgca gcaggtacac aggcattccg aatgccaccc aacctccaag cggcaagggg
     1801 ctgtcccagt acgccaaaac aatcaagcct gtcattggca ctagcaggta tgtcagtcca
     1861 taccagtgcc tgcgcagctc ccatttgccg attttcgggc ggcgataaga cctcagaagc
     1921 ctggttgttt gaaagacaaa aaagccgacg atcacaactt cgtacttcca tgaatcctga
     1981 aagcccgcca acaccacaat catgctgatg atggaggcgg cccatagcag ccaactgatc
     2041 caaatacggt tcctgagcaa gagaagctct gcctcattta tgtttgagtt ttcacttagc
     2101 atcgttgctt cacagaattc cccggcgaca gcgtcaatca taaaatttga tcaggtctgg
     2161 ctttgagaag atttaaagga ccaacaaaca gaactttttg ttcttcattg aatccatcat
     2221 tggatgatat accacaagta acatcatgaa actcaaactc gacctcgaag acttccgcgt
     2281 gccagacggc aaacccttcc gcattgccaa ggcgaagacg aaaatcaaaa acctctaccg
     2341 cgacgacgcg cactacgagc agctcttcag cgagttccgc gtggagatag acgacctcca
     2401 gcagaagatg tacgcgcagg accgccaggg gctgctgctc attttccagg cgatggacgc
     2461 cgcaggcaag gacagcacca tcaagcatgt gatgagcggc gtgaacacgc agggcgtgga
     2521 ggtccacgcc ttcaagcggc ccaccagcgc cgagctggac cacgacttca tgtggcggca
     2581 cctgcgcgtg ctgccgccgc gcgggcgcat cggcatcttc aaccgcagct actacgagga
     2641 ggtgctcgtc tgccgcatcc accccgagat cgtcaccaag atccagcgcc tgcccgcgaa
     2701 gaccacgaag aacctcgaca agctcttcgc cgaccggctc aaggacatcg ccaacttcga
     2761 ggcctactgc gaccgcaacg gcatccgcat cgtgaagttc ttcctccacg tctcgaaggc
     2821 cgagcagaaa aagcgcttcc tggcgcgcat cgacacgccg agcaagaact ggaagttcga
     2881 ggagggcgac gtgaaggagc gcggccactg ggatgagtac atggccgcct accaggacac
     2941 catccgccac accgccacgg acgactgccc ctggtatgtg gtgcccgccg acgacaagaa
     3001 aaacatgcgc ctcatcgtca gcgccgccgt gctgcacgag atgcagggca tgaaactcaa
     3061 ataccccgag ctgcccgccg agcagaaagc ccgcctggcc gccgcgcgcg agttcctggc
     3121 ggcggagaaa tgattccctg agcttgcgga ctccggctca atacaacccc agccctttca
     3181 aatgcaggcg caggatgcgg cgcatctcct cgacggcctc gggcttttca tgcacgccat
     3241 gtcctgagcg gacgatgagc tcggactcca cattgtccag atgcgagctg ctgtagggca
     3301 cgactccgtc ggagcattcg gggtcggtca aaggccggcc ttccttgttg ccgatgatgg
     3361 agtgcaggcg caccccaggg cggaaaggca gggagttggc aatcctcaca tagggcgagc
     3421 cgggggacag gttgtccacg ctggtgggca tgccgcgctt cagcagttcg ctcaaatagg
     3481 catcgcggga ggcgaggctt tccagaacct caaaggtgct gccaaaggtc tgccccggca
     3541 gcttcaccag gcttctgcca atgcgggcga gcctgccgtc agccatgctg ctgccgcggt
     3601 gcggcgtggc ggcaaagatg atgcggctga tgttcgggtt ggggtcgaat tccaaatatc
     3661 ggcgcagggc ttcatgctcc tctttggaga gtccgagagc cgccggcgtg tcgccaagga
     3721 tgttgaccca gacgcggtcg ccgctgtcct gcacctgcgc tttgctgaca aggccgccca
     3781 tgctgtggcc gatgagcacc atgtttctgc cgcgcatccc ggcgccttcc cgcgccagga
     3841 tgccatggag tgtgccaagg tgctcgcgca gcttttcaga gctgtagatg accggcacgc
     3901 cggaggggta gcgaaagaga tatacctgat aatgacgatt gaggacggga tcatcaccca
     3961 actcattgat gacgttttcc caggtggcgg ggcgggacat gaggccgtga accagcacca
     4021 gcgggatgcg gtttttatcc gggcgctcca aggcaaagag acccagcttg tccatgtatt
     4081 cggagctgcg cagcagggct ttgatgccct tcatccgcac gtctttcatg gccagatagt
     4141 gctcgccata ggcggtgaga tcagcggcga gaggctgctt cccgcccgcc aggatcactt
     4201 cttgagtcat gcgcgagtca tgcagagcca gcgtggccct gcgtaatccg ccggatgtcc
     4261 tggtaaagtc caatgtggcg gtgacggatg tcatgtagcc cgcctcagtc atgagaggtt
     4321 ccgcagcctt gcgctcggga cttgacttcc aatgggcgat caatggcgtg cccaccccct
     4381 cgcgcgtgat gcgccgcttc aggtgcctgc ttttgacggt gccggcagga atcagctcat
     4441 caaagagaag gttcgcgggg attcgcgtct ccagccggta agtttcatcc acatgaagga
     4501 cagctttttg atccttcgcc aggctgagcc agagcgcggt gaccctgccg acctgggcgg
     4561 cataagcctg gcgctgctcc acggtccgca gcccgcttgc cagtgttttt tcagcgtcgg
     4621 agagtgcggc ggacagcttg tcagcgaccg ttccgcgctt gtcggacgtg tcgtgaagag
     4681 cgtccggtgt catgctggag gtcgtgccgc atgatgaaac caccgcgcat aaaaggggga
     4741 tgagcaggta catgtaaatg cgcatgagat tggagggagt gaagtgttga aacgacgcct
     4801 caagcctcgc cattgaagcg gagggcagtc gggacgactg gcagtaattt ccttgcatga
     4861 tcgccagtcc gggcggcttt ttccattggc cctaagtcca acatcagaaa ataaacccgg
     4921 ctcgcacgcc gccgatgaca atccattcgg cctgcccacc gatgccgtcg ccggacacga
     4981 attgaatgtc gggcgtgatg atgagcgact tgccgatctg gcggcggtag aacagctcga
     5041 tgacgccttc ccgcttgctg gagttgctgc tggagctgcc ggcaccgaag gcgatgccga
     5101 ggcggtcgga ctggcgcaga tcgaaggcca cgcctgcggc ggcgaagtga tccgtcactg
     5161 cggcctcccc gtccgcccag gcatagcgca aaaaactgtg cgcgcgtccc gcgagttgct
     5221 gctcgaaggt gaggctcgcg ccacgcccgg actggatgtc aaactccgtc atcgcgtccg
     5281 aatcccatcc gagcaactgg acccgcgcgg gctccgcgtt ccagccttta aaattgcagc
     5341 cgatctgcgt ccccttgaac aaagcgcctc cccggaacct ccattcggcg gattcactca
     5401 ggttggtgga atcgagatgc gaggctccca gggtgacatc ccagccagcc cggccttccc
     5461 aacgaacaac gaaacccagg tccgagcctg gaaaacccac cgctggactg gaggagaatg
     5521 cctggttcat gaacgagcgc ttggcgctgc gcagttgatg accgtcgagc aggtcatcaa
     5581 tggtcatctg gccgtagcgg agcgtcagcc gttggtcgaa gaatttgtcc tccaggaaga
     5641 tttcagggac ctggaaaccg gcgttcgtaa acccatctac atggccccaa agcgcgcccg
     5701 tttccgtgcg gagatccgag ggtgcgaggt cgctgcaggc gtgacgatgc cgcatgcgca
     5761 gattgaggga caggggggcc ttgctggtgt ccgcattgat ctgccaccgc aggttcaagg
     5821 tgccgtctcc cgaagaagcg ccccagccgt cgtcgcgccc gatggcaccg atcgccagcg
     5881 aatcgtagct gagagcgatg tccagccgca gcgcctccat ccacgcggtg cgtcgttgct
     5941 gccgctgctc ccttgggttc cgccaatgct ggtcacggat ggcaatggag gcggcgatgc
     6001 cgtcctcgtt gcgcttttca tcggtgagga cctcttcctg cgccatcgcc gtggtcgtga
     6061 gacaggcgaa ggccgcgagc agcaaacata cgggaagcgg tttcatcctt gaggaagcat
     6121 ggagcgcggc ggttctcccg tctcggcacg cccatcccaa tgcctttcgt gctgggcgag
     6181 cagtccggcc gagcccatgc cccactgttg gagcacggtg gcgatgctgg tgatccggac
     6241 atgcaggggg cggacgcatt ctttcgggac gatgtgcacc gtgccagaag cgggattggg
     6301 cgtttcagga acgaaaatcg tgagttttcc gttcccgtga tcctctgtga tgaacacgaa
     6361 gctttcgtca ccttcgccag tcttgagcag accggccttc accacgccct cccgttccat
     6421 gctggccagc ccgccgacga tggcccgcgc ggcagcatat ccggggacac ggtagagcag
     6481 cgtgcgctca atccaggtgc cgaccgtcct gccctggcgg gaaatcatca tcaagccgag
     6541 catgaacgaa aggccaacga cgatcagcac ggcgtagatc atcggaaact cggccgcgac
     6601 cgaatcgtgg cctgccagac tgtccatgac ggactgggcc gccgaatgtg tggtgacaat
     6661 aactttcccg acaaggatga gaatcaccac caacggcagc agcataaaca gcccgccgag
     6721 cgtggtggct ttgagaaagg cggcgattcg tttcatggcg gggctgccgt catcgcggcg
     6781 cgttcagctc aagcagacaa ggcgtcccgc cgcctccaac gctggcagcc acgcgagcat
     6841 gaagatgaag acggcaggaa taaaaaactc atgatcgctg ggggggcgag tgtggagctc
     6901 aacgacggcc gccgcgcgag cgtgaaacgc tggaggtcga ccgtgacacc gtgctcgagc
     6961 gtgagcgcga gtaggcatcc cgattgagct gcgagggcgt ggactggcgg ttttgatagc
     7021 tgccacggtt gaacccccca gaagaaggcc tagccgcagg ctgcgtcgtc ggacgcgtga
     7081 cgggttgcgc aggcttgacg ggagcttggg ctggacgcgt cacaggctgc gcgggcagag
     7141 tcgtgggacg ctcggggcgg gaagcgggag gcgaggtgcg cggcgggtag ggaggattgt
     7201 ttgccggcac gttgggtgcg ctgttccagc cgccgttgga gcgtgagtgc cagccgtttt
     7261 cgtccttctt gtagatgttg ccgtccttgc cgacatagac atcgccgctg ccggttttcg
     7321 ccacgaaggc atcggagccg tatttgcggt tcacaccgac cgccgcgccg ccgcgggagg
     7381 tctgcacgcc gccgacggtg ccgtaggcgt ttgagcgatg cccggcacgc acccattcat
     7441 catcccgcac cacggcggag cggccccagg agccgtaggg cgtctgaacg gccacacgac
     7501 ccgccgctgt gttggtccaa gggttgtaag ccgtgcgggc tgccacggct ccgcgtggtc
     7561 cataggcata cgcaccgcgt gagtagatgc cggtggccgg attgtaggcc gcgccgtagc
     7621 cagcaccacc gtaggggccg tagtagcgcg cgccgcagcg atagtagccg cgtcccggat
     7681 gataaatggc accgcagccg taaccatacc aatacgggct ggggtaataa tggtggtgcc
     7741 aatggtcatc gtccgccagc tcgtggccca gccacatgcc gagtccgaag accagcagcc
     7801 cgcgggcgac ataagcgccg gagtaaccgg aggtggcgcc gacttgcacg gtcgtcggtg
     7861 tggaattgta cacatgcaca tgggtcacgt tgtacttcgg gctctgcggc gggatggtgt
     7921 agatcggcga aggcacatgg tcgcacacga cccacgctcc gccggcggca gaagcctgga
     7981 accacacgcc ctgatggcag cagtaataga tgccgttgac gagaaacacg tcatacgagg
     8041 tgttcgtggc atactgcacg ccttcggagc cagggatggc cttgaacttc ggctcgcctt
     8101 catacgccac ctgcaccgtc gtgctggcgc gttccacagt ggccgtttgt ggaatggagg
     8161 ccaggatcac cgcctcgtcc gcctcgggtg ttcctggaac ggaagcaagc acatgcgcct
     8221 tgggatggtt ttccgggatg ttggcgaaat cctccggcag cgtgtccatc gccgatgccc
     8281 acgggccgtc gaggctcgcc gcgctgaacc agcggccggt cgtgagcagg tagtagctct
     8341 tgcctgcggg atgcaggaac agatcgctct ccgtgttcgc cacatacatc agcgaagtgc
     8401 ccgaaatcgg caccaactgt ggttttcctg ccgtgacgat gatctccgag gggcggttcg
     8461 cggcgaaaac acgcggcgcg ggcgtgctgt cggagggaag ctccactttc gccttcacct
     8521 cggaccagtt ggcatcatcc ggcagcttct tgaacgaggc gggcacggtc ttggcgacct
     8581 tccacgggcc tttgatgacg tcctgggtca ccagccactg gttttccgtg agcagatagt
     8641 actgcccgtc gttgaagatc acgtcccagt tcgtgttcac ggcgaacatg agggtcggat
     8701 cgtcctcgtt caccgcctcc agcttcggct cgccaatgaa ggtcacgagc acggcgggcg
     8761 agtcgctgta aaaaatgggc ggcggctcaa aactcaccgc ggtgtccgcc gtggtggcgt
     8821 catcgcggtt cacgacggcg atcaccgcag ccagcggcat ttccagcggt gtctcgggct
     8881 tcaacacgga gcgcaccagc tcctccgctt tggcagcgcc ggcggcgtcg agctcgggaa
     8941 agcggaactg ggtgaactgc cgcgccccga cccgcacggt gtcgtgctcg aagtccgtga
     9001 tcgtgtctgc ttcgatggcc acagcaccga aggtggcctc ctccttgcct tccagcttca
     9061 cgccgatggc ggaggtgccc ttcaaatggc ggaactcctg ccattcgaga atctgcggca
     9121 tgtagatggc cacctccgca ccgccgactt catagacgcg cggccaggca tcgggattga
     9181 ggatgttttc aggtgcgcca tgcgtggcgg tcacggagag gctcgtcaaa agcgccgtgg
     9241 tgaaggtctg gagaagacgt gatttcatgt ttttggggta gggttaaaat gagaggcgtt
     9301 atttggtgat gctgactgcc accgactccg tggttcggaa gaccacctcg gcaccgacgg
     9361 cgtgccgcgt catgtccaca tcaggacgcg ctttgaaggt ctgcctgcga ccgtcggcaa
     9421 agacgatggt ggcggtgcgg ttcttggcat caatggagtg gacccggccc gtgtattcgc
     9481 tggtgttcac gatcagcacg gagggtttcg ctcctttggg tgccacgacg accttctgcc
     9541 gcacttgctc ggcactcgcg ggcatctcgc tgccgggttt gcggacaaag ctcaggtatt
     9601 gctcggtcag cgcagcgcgc acgcggtcac cgggctcgat ctggtcaaag ttccgcacct
     9661 cggggcctgc gcgataggtg ctcttcctgc cttgaggatc gaccatcgtc acctcccgcg
     9721 tggatttatt gaccttcacc accgtggcag agagctcggt ggtttcgaca agcaacccgc
     9781 ccggctctcc tttcgtgatg atcgtctcct tgctcttgcc atccgcgagg aggctcgtgg
     9841 cacatcccca tccacccgcc accagcagac agcccagcca gcacgagagt gcctggtgga
     9901 aacgaactgc tggcagaggt ggcgtggtca tgacaaagct cacggttgct ttaccaaggc
     9961 ttcaaggtca cgcggctcgt gcgcttgatg gtctcgtccg gcttggtgtt gaggaagctg
    10021 gcaaactccg ccgcccattc gctgatgttt tgctcagcac catgatacca ggtgtatttg
    10081 ccgaggttca ccggagccac cttgtccgtg cggcggtcct tgaaggtggc gatgatggag
    10141 ttgtccgctc cattacgggc ccggccctcg atggcgatgc tcccgcgtcc tgccatggag
    10201 aggtagcctg caccggggac agcaaaacca gccgccgtgg aggccgcatt ccagaaggct
    10261 ttggaaggca ccagctccac caaagccagc tcgatgacca gcgtgtccgg ccctgggctc
    10321 aaggccagct tcatcttgtg ggctggatga ttggcgacag cagtcatgaa ctcatgacgg
    10381 agccgcgtgg cgatcttccg cgtgtcctga gcgaggtcat tctggcgcag aggcgtctgg
    10441 gcctgccacc aagactgctt cgccatgtgc tgcgtgttga ccggggcaaa atagacggac
    10501 ttgaactttt ggctgaccgc cgtgccttga taggcaggag aaacccacac ctcttgaagg
    10561 aatgccgcac gggtattctt cgtggcagtg gtgggtggat tgaatccgga gtcaggagct
    10621 tccttcgttt cgagtaactt gcagctcgtc agagcagcgg agaaaagcac gacggacaaa
    10681 caaggaataa ggcaggttgg tttcatgatg tggtggtgat tgagcccgtg gtcgcaaggt
    10741 cagtgattca gcctagccat ctttgccacc gccgcttatt ggacctaagg ccaacagact
    10801 tggcgctctt tgatgcccgc catcattcaa gaaggcgggc cgtgtgcgga tggtgtgagc
    10861 tggcagatgc ctgagccttg cccaggcagt ttagaatttc agtcccagct cgatctgaac
    10921 gccgctggtg atgacatcat aggtgaagcc gccatcggtg tagtccacac cgagcgcacg
    10981 gtagccaaga ccggcgtaca tgtgcttgct gatctgatac tccagaccgg cgtaggcctg
    11041 ccaggtgatg tcggaggaaa caccgaagcc gccgatgtcg cccttgagca ccagggtcag
    11101 aggttctgcg agcttggcac gggtgaggaa gccgacatag gggtccaccc agcccttgtc
    11161 accggagatg tctcggttga gcactggcag aggcgaagtg atgttcaggc cgaggtcaat
    11221 gtggttgtaa cgagcaccgc cgagcagttc gacgcggcag cttgcaccat ccaccagcgc
    11281 atagctgacg gcggcatcaa tcatcaactg gcgcatctcc agcgtgacgt tgttgatcag
    11341 cggaccaggc gtgcccgcag cacctgacag ctccatgtaa gccaggccgg tgagcagccc
    11401 ccaacggcct ttgcgggctt cgaaattggc agcggccacc atgtccagat tggagacgat
    11461 gtcgtcaaac ggcacgtcga tggcgctgac ggcgccgaag acgccgacat caccctcaat
    11521 gccagaaatc cagccgggaa ggccgaggcg aaattcccag tcactggcag cagggatggg
    11581 ctcaagggtg gctgcgggag cgggtgtccc cgcgtgggct gaagctgtca atgcaatgac
    11641 ggccatgaga tggtgcagtt tcatgatgat gtgggtggtg ttggtttcga gggtggggaa
    11701 gcgaaggctg atgcgccgcg agcgaatgac ggctctccga catccaaaac ccactggcag
    11761 cgtcttaaag tctcaccctc cagctgatgc ctctattgga ctttgggcca acatcacccg
    11821 gccaccggca gtttgattcg aaaaacagcg cctccttcgg cgtggttctc tccgcacaac
    11881 tcaccgccaa agcgcttgat gatgctctgg ctgatggcga ggccgaggcc cagcccggtg
    11941 ggtttggagg tggtgaaggc ttcaaacaac cgatccaaaa cctgcggcgg gatgcccggc
    12001 ccattgtcct gtacgctgat gagcacacga tccgcctcca gggcggtgct gagggtgatc
    12061 cgcggctgag ccaccgccgc gccccgcagg gcatcgaggg cgttgagcag cagattcatg
    12121 agcacctgct gcagctccac acggcccgcc ggcacgcatg gcagcggccc gccgagtcgc
    12181 aggtccactt tcactttgcg ggccatgatc tcgctatgca ggaggtcgag cagttgcttc
    12241 acggcctcgt tcacatccac ggcctcgtcg ggcacggcgc cttccttttt gaacatggcc
    12301 ctcatgccgt gaatgatgcc cccggcgcgt ttcgcatccg ccgtgatgtc atccaggatg
    12361 gctttcatct ccgtcctgtc cggcttcggc tcatccatca tcgaggagcc gacctgtgaa
    12421 ttgctcagca tggccgccag cggctggttc aactcatgcg cgatggctcc tgcgaactcg
    12481 ccgaggatgc tgatgcggga aagccgcgcc acctcctgct cagcctgcct gcgctgcgaa
    12541 atgtccacga tggcggcgag ggtcagcggc cccgcctccg tctcaaattg gctcaaacgg
    12601 atctccaccg ggaagagcgt gccgtctttc ctgcggccaa acctttcccg cccggtgttc
    12661 atggctttcg tttcagacgc cgcatgaggc cagtcgggga tcagcgtgca catctcccgc
    12721 ccggccagtt cctcgcgagt gtggccgaac catttttccg tctgctcatt caccaaaacg
    12781 atgcggccgt cctctttcgc gagcacgata gcattcggcg atgattccaa ggccagccgg
    12841 aatttctcat ccgcgcgctg cctttcgcgc aggcgctcca attcctcgat ttcctgccgc
    12901 cgcctggatc gcagccgcca gcgtgcccac tggctgccgg aggccacgag caggcccacc
    12961 agcagcagcc tgaaaacgcc tgtttcccaa agatgtggct gcatcgtgat ctgcagccgt
    13021 gccgtggcag gctgccaccg caggtcagga ttcgccgcgg tcacttcgaa gacatagtct
    13081 ccggcaggca gatcactgta gcgaatggat cgcacgccca tgtcctcctc ccaatgcctg
    13141 tcctcggcca aacggtggcg aaagtgaacg ccctcaggcg cggcaaaatg gaaggccgtg
    13201 tactcgatct ccagatcaag ggtgccgggg ggcagtgtca gagaaccccc gcgtgtcgtg
    13261 actggttttc cacccactcg cactccttcg atcatcacct tgggcggttc tgtctccaca
    13321 ttccagtccc ttggattgat ggcgatgatg ctgcgtccgg cggcaaagtg gagcacacct
    13381 gatgcgctgc gaagacccgc cggtgcatag ccgcccgagc attcaaagga catcattccg
    13441 tgagatttgc tcagcaacag cgggtgcagc gttcgggcct tcccggtcgc cacggccttc
    13501 aactcgtctt ttttcacccg cgtgatccca tgatacccac ccagccaaag atggccctgc
    13561 tcgtcctcca aaagttggga catgacattc cctccaagtc cctggcgagc gtccaccgaa
    13621 taaaatcgac cattctccaa ccaagcgagt ccgccgccgc cggtgccgat ccagagagtg
    13681 tcatccgcat ccaggtgaag gcacgtcaga atgtccgtgg gcagtccctc ttgggtggtc
    13741 cagcgcttca cctggcccgc agaccaatga aaaagcccgg cacgtgtggc cagccacatg
    13801 gaatcgttcc ggccacggac cagtccctga atgctgtgag gaaacttgcc cttctccaca
    13861 gtgtgaaaaa cacggccctc caagcgcttc aactcacctg cgttggtgcc catgaaaagg
    13921 gaaccatcgc ccccctcgca cagggcacgg ggaatgtccg aaatcaccgt cgtttcgagc
    13981 acttctgaat taggggcctt gcggaccaga atctgatcac ccgcgaagaa gatcgtgccg
    14041 tcacgcatcc gcagggctgt ccgcatgagg ttgccgccca ccggttgatc cccgttcatc
    14101 accccgttta gatgccccag agaaccatga tacagacccg tgccataggt gctcacccag
    14161 aaagtgccat ctggctcctc caccaagctg ttgacgcctc ctcccatgct ctcatccgcc
    14221 aggacatagg atctgacctt gccgcgcgtg agtcggaaga gaccctttgc atccgtgccc
    14281 acccacaccg tgccatccgg catcacaaag agtgagcggg tctctttgtc cgtgccgggc
    14341 acaggacgaa cgacgccgtc cttcatgacg tgtagcccgt catcgtagcc ccccagccag
    14401 acttcgcctg aatttcccgc cgccatgcaa aatggagcca ggtaaggcac gccgttcgct
    14461 tgattgaact ccgtccactt accgtctttt cggcgaagca tcacatgggg ggtcatgcct
    14521 acccacaagg caccatcccg atccgccagc agggacgagg ccgaaagccc ccgagaactc
    14581 ggcagtggct ccacgacctc gcaccgttct tgatgcacat gaaccagccc gagattttgc
    14641 acgcggatcc ataacccgtc cgaaggtgtc atcgccatgg cacccaccct gccgcgaaca
    14701 ccttcggatt cgcccacctg cttgaattta ttgttctcaa gatgctgcag accctggtcc
    14761 gtgccgatcc acaagccgtc cgccccatcc cgcgccagac acagcaagtc attggtcacc
    14821 aggccgtcgg ccgtcgtctg cgtggtgatc acgccatcat gaagatgact gagcccgcct
    14881 cccatcgtcg cgatccagag cccgcctttg ccatcgtcca ccagcgaccg gacgtgcaat
    14941 cctttcaatc cttctgccag cccgaaatgg acgaaacgaa tcccgtcaaa ccgcgctagt
    15001 ccgccgcgtg tgcccaccca tagataccca tcctgcgtct gggcgatggc cgtgacggta
    15061 ttctgcggca gcccgtctgc tgaagtccac gacttgacga taaaagagtc gtcgtcagcc
    15121 gccccaacac aacaagctga cattgccatc agcaataaaa tggcacgcca gttcatgagc
    15181 gagtgagagc gggtggacgc gaggagaaca atgccgccat gctagcagcc gcgatcaagg
    15241 ccgccaagat cgttattcac ttcgcgtgcc atcaaaaccc ggtcgtccca cccgaatgag
    15301 actggaaaag tccgtcgatt ggtccgccgc cctggctgct ggattgatga gcgctttttc
    15361 acgcagcgcg gcaaaaggca ggccgccggt ctcaatgtag ccgcgctcat agagcaggct
    15421 gtccgccttg ccgttgacga ggatgcgcca gtcccagggc gggcgtttcc cggccggatg
    15481 ctggccgcgg atggccgtgg tgcagttggt ggtggcggcg ttgtaccagc gcggctgctc
    15541 acgcagcgcg ttgagggtcg ccaggtattc catgaaccgc tcgcgggcct tctccggcgt
    15601 gaggcgtgtg cggtagaggt aggattcctc gccatggcgg atgctggtgc gcaggcggac
    15661 gacatcccgc tcgtccgcag cgacgacata gagctcgaac tgccggtaaa agccgccgat
    15721 ggcggagtaa atctccccaa cctccttgcg cgtctcgatg gaaaagcaca gcggcggcgc
    15781 atcggcgaat tgaaagctca caatgggatg cgccatccac ggtgatcccc agtagttgat
    15841 gaaaaggtcc acgccggtta tttgagagac gcgcagcgtg cgtgtctccc aacgcggtgt
    15901 gaaatcggtg gcggtgcggt agtcgaaatg acgcacgttg tgcagcgtga cctcgtcgcc
    15961 ctggatttcg gcccagggca gctcagccac gtccgactgc cagtcgcgat cagcacgcgg
    16021 cgagagcgtg agccaccagg cgagcacgag gccgcagagg gcaaaagcgc aggcggtttg
    16081 cttccaaaag ccgcgcagca gaaacaaggc cgtcatcaca cccacgacga aaagcccggc
    16141 cacgacatcg tgaaacaccg ggaagtcaaa atagagcgcc ccgaaggccc atgcggtggc
    16201 gagcagcagc aaaagccaga caaggcacct tcccagtttc ttcccaactc gaacagcgtg
    16261 cttcatggat cgtgtggtgt gaaaacggcg aaggaaacgg agacccggcc tggctgcctg
    16321 atgtccccgt ttcctccgat gcatggttta cttctgctcg gcgtgaatgt ctttttggcc
    16381 gacgccttca tccgtgatgg agtgaccgtg aaccggctct gcggcgggtt gtttgccatc
    16441 gggagaggcc atgttgggct tgccgtgggc gaggccgact ttggcgacgt acatgagggc
    16501 gagcggcttg gcggattctt tgccgagcgc gttgaggcgt tcctgcatgg cgtgaatctt
    16561 gagcggatgc ttcgaggcga cgttgttctg ctcgtaggga tcgtcggcga ggttgtagag
    16621 atcgacgctg gtggggatga gggtgcgcca gatcatcttc cagtctccct ggcggacggc
    16681 accgcggaaa ggctcgacat tataaacaac ctcggtgcgg ggcgattctt tgcctttggc
    16741 gatcgtgtcc cagacgttca cgccatcgag cggtttgcac ttggcggtgg aggcaccggc
    16801 gagagctgca aaggtgggat agaggtccac ggcgtggatg atgccgtcca cggtctgcgg
    16861 cttgatgtgg ccgggccagt tggcgcaggc cgcgacacgg ctgccgcctt caaaaaggga
    16921 gcctttgcca tcgcgatagg ggccgttgtc gcagggcagc acgactttgg agagatcggt
    16981 catctggccg gcaaacatgg cgttcttcgt gccgccgttg tcgctgtgga agatgatgag
    17041 ggtgttttca cgcaggcctt ttttgtcgag agcgctgatg acacggccga tctcgtcatc
    17101 caggcaggaa accatgcctg cataagtgcg acgggtcggt tccttgatgt ttttgaagcg
    17161 gtcgatgtac tcctgcggtg cctggtaggg cgtgtggggt gcgttgaagg tgaggtagag
    17221 gtagaagggc ttgtcggcgc tttgctcgcc gaggtacttc acggcgtcgt tgccgatgag
    17281 ctgggtggtg tagccctcct ccttcacggg ctggttgttg cggaaccagt cgagcacgcc
    17341 ggcatcgctg tgggtgtagt agtccagctc gccgatcatg gcgccgtact ggtagtcaaa
    17401 gccgcgctgt ttcggccagt atttgatgtc cgcatggccg aggtgccact tgccgatgat
    17461 ggcggtggtg tagccggcgt ccttgaggca ctgcggcagc aggtgctcgg tggtgtcgag
    17521 gccgtagccc gccgggccgg ggatgacgat ggtctgaagg ccgtagcgga agggatagcg
    17581 gccggtcatc agcgcggcgc gtgtgggcgt acacatggac tgggtgtaga actggctgaa
    17641 cttggccccg ccggaggcca gggcgtcaag attcggagtc ttgatgtccg tggctccatt
    17701 gaagccgaca tccttccagc cgaggtcgtc ggcgacgatg tgcaggatgt tgggtttggc
    17761 cgcagcctct gtttctgtga cggcggtgag cgccatgccg agggcgagca ccaggagttt
    17821 gccgatgtgt ttcatgatgg gtgtgttgtt ggaatgagtt gaggtgagtg ttaaggcatc
    17881 agggcgccgg gtagatgcgc gtgatcttgg agccctggag gccgaggccg cccatgaggc
    17941 cctgctggtt gaagaagatg gcgtagatgc tcttctggat ggtgttggtg ctgagagagg
    18001 ccgccatgcc ctggtccagg atgaccaggc tgggcgagct gccgacctcc caaccctggc
    18061 tgctgttcag agagcgcaga gcgctgtcat ccatcaggaa gagcgcataa ccataggtct
    18121 gcactccggc ctggagaccg taggaaaggc cgacggtctg gtaaaagtca cggatggcgc
    18181 cattgcgcca gaccagggag ccgttgccgc ccatggcacc gaccatgaaa ccgcctttga
    18241 cgatgctcgg gaagacgagc acggctttgg cctgcgagcc tagccagcgt gccttgggat
    18301 tggcggcata gagctgatgc agggcagcac gggaggagga agcgatctga gccttgctgg
    18361 aattggcggc attggcctga gtgagtggtc cattcgcaca actgctgagc gagagggagc
    18421 tgacagccag gagggtagtg acggcgaggg tttggatggt atgtttcatg tgttggattg
    18481 aggggtgatt gagaagacca tcaggcgtgt tcttgggtgt tttgttcgtt gagcgaccat
    18541 cgcacggcgt cgagcagcgc ctgggtgtcc acgggcttgc ggaagtagcc atcggcaccc
    18601 gcggcctgag ctgcggcgcg tgattcggtc gtgtcatgcg cggtcaggaa aatgagcggc
    18661 agaggtgaat gcgcggcgtt gagggccagc ttcagatcca ggccgctcag gcccggcagt
    18721 gtcatgtccg ccacaatgca gcccacggat gtctgcgggg cttcattcag gaagtcttcc
    18781 gcacagtcat aggcctgatg atccagtcct gcggatgtca tcacacgtcc cagcgcgcgg
    18841 cggacgctgg tgtcgtcatc aatgataaaa atggtgggcc gtcggtgtgg cattgcgccg
    18901 ccacattgtc tggatgggaa aagccctgct attggacctt gggccaatac acggagaact
    18961 cacactgggc ctcatgaagg ctctgcggct gcaaagccaa cgagcgcaca gagtctgacc
    19021 agctcggcga cggactgagc ctgcattttt tccagcacgc gggcacggtg caccttcacg
    19081 gtcttttcca cgacattgag ttcagccgcg acctgcttgt tcagcgcgcc cgtgatgacc
    19141 cagctcatga cttcgagttc acgctccgtc agtgttttca tgcgctcacg gatgctgctg
    19201 acctgctgcc cggcagcgag cgtggcgcgg tgcttttcca atccttcacc aatcgcgcgc
    19261 aggaggtcgt cattgtcgaa aggcttggtt agaaaggtca ccgcgccctg ccgcatggca
    19321 cggacggtgg aggggatgtc accattgccg ctgatgaaga ccaccgggca ggaggagtgg
    19381 ctctccagca gccgctgttg taattcgagt ccgctgagcc caggcatctg caagtccacc
    19441 accaggcagg cgggacggga tggcggagcc gggtgggcct ccagaaacgc ctccgcagaa
    19501 gggtaggtcc gaccgtccag acccgcctga cgcaccagcc gtgcgaggct ccggcaaatc
    19561 gaagcgtcgt catccacgat gaagacagta ggtggggtga ggctcatgca gtcccctctc
    19621 ttttgtctgt catgaaaacc tctggcaagc gcctttttgc agcgtgtttg aagcacaccg
    19681 caccccgtcc gacccagcta gaagagcttg aactgcatgc tcatctggat gcggtgggca
    19741 tcgccctcgc tgccatcacg cgtggttttg gaaccgtaga gataccccag gccgacgtaa
    19801 aagcgcttcg aaggtgccca gatgaggttc acctctgaat acaaggtgcg ctcataggct
    19861 ttggggcctt gctcgtctgc cgtttccata tcgacggtgc caatgctgaa ctcggagcgc
    19921 caccggtctg cccaaaagtg acgataaccc acatggccgc cccagccaga gagcgtgttg
    19981 aggcgtccgg aggcatccac caccgcaccg ctgccggcag gcagatcctg aatgtagcgg
    20041 cccatgccgt caccgtaagt caggctggcc acgaggctgt cattgccccg cagattcaga
    20101 gtgcctgaga tgttggttcc ccatgcgaac acggagtcct ccgccccggc ggctccttcc
    20161 caggcgagcg agcgtgctag tccacccacc tgcacatgac cagcagcgcc ttccagccgc
    20221 cagtgaccgg tgatgtccgg catggaggtg cgcgtcgtcg cgcctgcggg cagggcgccg
    20281 atatcgctct tcggttgctc cacggccagg gccacatgca tgtggtccgc gatcggtgaa
    20341 aaagtgtagc gcacctgtgg ctgccgcagg atggactgcg agccggggcc ggcgaagtcg
    20401 agcgtgtccg ggatggtgtc gggatcaaag aaggtcgtcc atgtctgccc gacggtgaca
    20461 tttgccacct ggccgtagaa gtgacgaagg ttgtactcca tgtcagggct ggcgctgttc
    20521 gcgaagaaat cgttttcgta aaagaagcgt actgacccga agggtgaagg ggtgcgcagt
    20581 tccacgttaa accggctctg cttggcatga agggcgtact cccggccctt gccaaaattg
    20641 gcctctccct cgacgggaat gccgccagtg ctgaatttgt tagggttccc catcttggtg
    20701 gaatccacga tggagtccac cttggcgtaa ccgccgagtt tgatccaggt cgtggtgtct
    20761 ggaatgtgca tgaatccctg gtaagtgggg tccatcggcg catttcctgg ccggggagcg
    20821 gagaggttgt cccccgtcac ggtctcccga tgccgcatca tggatctgga gtcgctcagc
    20881 ttcgcggcat ctggtgttgg cttggttttg acagcctctg ctggcgtgag ggacggcttg
    20941 tttgccgcac tgaggctgac ggctggagcg gggcttttct tctgcttctg aagttcggcg
    21001 atctgtccct gcatttcctg catcgccttc tccatccgca tcaccgtggc gcgcaggcgt
    21061 gccagctcgt ccgcttcatc ttcggcatga gcctgggggc caaaggcggt ggcgagcagg
    21121 gccatgccaa gcaggagaga tgtttggtga tgccttttca tgcagcgttc cgaggggttg
    21181 tctggcttca aggcctgacc ttgttcacca tggtgcggga gccgatgatg cggccattga
    21241 gcggctgcgt cggacggttg ctgggactca ggcggctggc gaaagcctcg atctgcgcct
    21301 tgctcgcgga aatgggatca cggaacacga gccactcgac ttcctccgtg catggcggtg
    21361 tggtaagcga gcccatgtag gtgtaggagg ccgtcgtggc aggcagatgg aaggagagtt
    21421 ccagctccac ttcgggcagg tgggcggagt cacccttcgc cttgggcaga tgagggatga
    21481 ttttgccgag gttttcattc tccgagcctt cctggatgag cgcggcgacc accgcaaact
    21541 tcccgtcggc gctctgatgc acaaaatgca tctccatggg gaaatagctg ccatcaacgg
    21601 tgtgctcgct gggcgtgtgg aaatgaaact gcttcagcga atagacacca cgatccgtgg
    21661 tgagagtgct gccgccatcg acgctgacct ggatcgtgtg cccgttgtcc acgatgtcgg
    21721 tgacatgctc gtgatgggcg attttgagcg agctcttctt gtaatccagc ttccaggccc
    21781 tggcgtctcc gtctgcggtt tgggtgatgt tgatgggtga ctggcttttc ccctggccgc
    21841 aggcagcata cgcagggttg agtgcaggcc aatactccgg cccgtccttg ccttcatagc
    21901 cccagtgtgc cgggcgggcc gtgccgtgct cggtgccggg atttgatcgg tctttgcacg
    21961 ccaccaggat gctgaaaagg accagtgcaa acagaaggga gggatacgtt ttcatccctg
    22021 ccttatcctg gatcattctc gttctgacca ttggaccttg gtccaacaaa caccgccact
    22081 ccgttactcc ggcactggca aagtcatttc aaacaccgca ccaccttccg ggtgattgcc
    22141 gccggtcagc tcaccttcga agcttttcat gatgccctgg ctgatggcca gcccgaggcc
    22201 caggccgcca ggctttgaag tggcaaatgc ctcgaacaag cgcgcctgaa tttcaggcgc
    22261 gatgccgggg ccgttgtcca ccacgcggat gacgacagaa ccggcccgca gctccgtggt
    22321 gatgccgacc tcgctgctgg cagcgcctgc ggacttcatc gcatcgagac tgttgaggat
    22381 gaggttgatg agcacctgct ggatctccac ccggccggcc atcacctgcg gcagagactc
    22441 ggccaggttc agcttcacct tcgccttccg caccacgatc tcgctgtgca gcaggctgca
    22501 cgtttgctgc accgcctcat tcagatcgac cggctgcacc tgcatcgcgg tttccttttt
    22561 aaacatggcc ctcatgccgt ggatgatgcc gcccgcgcgc tttgcatctg ccacgatgtc
    22621 ctccaaaatc gccgccatct cctccatgtc cggcttgccg ctgcggatca tgctgtggcc
    22681 tacctgcgag ttgctcaaaa tcgccgccag gggctggttc agctcatggg cgatggtgcc
    22741 agccaactcg cccagcatgc tgacgcggga gagatgggtc acgtcctgcc gtttctgggc
    22801 gagttcctgc tcggagtgct tccgctgggt gatgtccatg gataccccgc gcagcagcaa
    22861 gggcctgccc tggggggttc gctccagtcg tcccgcagca ccgatccagc gctctgaacc
    22921 atccggcagc acgacacggt attccgcggc gaagggggtg gattcatcca aggtcgcgcg
    22981 cacttccgcc tccaaagctt cgcggtcatc gggatgcacc gtgctgatga agcgcgggaa
    23041 atccaccacc tcatccggcg ggatgccata gatcctgcgg ccttcgttcg tggtccagat
    23101 gacgttcgtt tggatgtccc attcccaggg tgccagttga gccgcactgg ccgccatttc
    23161 cagcctctgc tcgctgtcct tcaggctgct ggccagatgc gccgcacgca gcacatcccg
    23221 gctcagctca taacccatca cggtgagcag gatgagaaag gtgagagtca ccgccaccgg
    23281 cagcgcgatg acctgcgcca cgaccaggga tgcctgtgcc agcccggcgg tgaggatgaa
    23341 gatgatgcct ccgccgatca tcaccacccg tcgtctgcct tcccgaccac cggtgcgcca
    23401 gagccgccag ctcgcgtcca gcaggtagcc tgtccagatc accgccgcca gatgctccac
    23461 ccagagccag cggcccatct caccaatcgg tgcggacacc gtttcgccca gaaagctgac
    23521 cttgcggatg tcggtcaccc tttcaaaaaa caaaccatgc gggcacagca ggttcaccag
    23581 cagcgcagtt ccacggctca ccagcgccgc caccagcagc cagcgccttc ctgtgccgaa
    23641 gtagcaatgc accagcatga ggaaggcgca gatgaccagc gagatcggcg tcagcgccca
    23701 gcgtgcggcg cgcccgaatt cctccggcgt ctccgccatc atcatctgga gatcaaaaaa
    23761 cgggtaggcc gtcaggcagc ccgccgccac ggcgaaccac agctcggcgc ggaccttccg
    23821 attcgacagc cataccatcc catggatcac ccccagcgac agcgaagccg ccgccgccat
    23881 ggaccaaagg atgacaatca ggctcacgtt gtcttttcaa gcaccagcct tgcgcggcaa
    23941 caagagtttt ttccgacaat gacagtccag gcattcaagg gcggagtcct gatgctgcaa
    24001 acccgcctcg gtcagttcag ggcgactgcc gccgcatgag tgccgcatgc tggagcagca
    24061 agcggtagtt gtccggccag cgagcggttt cctcgtcatt ccaggcgccc gcctcggacg
    24121 cccacagata ggcgcgctca gccctggcaa aatcccccag ccggtgccag tggatgccca
    24181 gcgccaggcg cggctcctca tgcaccggcg ccagcgccag cgcgctgcga acatgcccga
    24241 acgcctcatc gtggcggccc agcgcgtcgc aggcgtcggc cagtgccaga tgatgcaggt
    24301 agcgcgcggg attcaatgcg acagcctcgg caagtgcggc ggcggcggct tcgaggtctg
    24361 cctgcgcggc atcgcgcttg tcagcgtcca ccttttccag aagcgcgaga ccgcgttgat
    24421 agtgaacctc cgggttgccg ggggacgctg ccgcggcgcg ggcgagcatt tgcaatcgct
    24481 cggccctgtc ccctcgcacc tgagccacgg cggcgcgcgc caggaagtag tctccacgcg
    24541 cctgggtcca cacgcaggcc agcagtccga aggaaaaaac cgccagcagc gccttggccg
    24601 caaaccgcgc cccaggcagg cggaccggag cgcgcttggg gtcgtccaga ccagggttgg
    24661 ccaggatgcc gagcagcagc gctccagtca gcgccgtggc cgggacgtga aaatgaaact
    24721 ccaccgccgc atgggccgcc gtggccgcga gcgcggccag ggatccaacg gccaggccca
    24781 gcgagttccc tgccaaccgg cccgtgaccg ggtagcggtg ccggatgaac caccgcagaa
    24841 atgacaggcc gctccatgca tgcgcggcaa gcatcagcaa cgccagagca agacccgccc
    24901 agccgtagtc ggccagcatt tgcagatact cattgtgagc gaaaagcggg tccgccgcgg
    24961 tcaccggaag ctccggtgcg cgcaggcgga tgctgccctc gtaaaacatg cgggcccccg
    25021 ctcccagcca gggcgcctcc tcatgctggg ccagcgcgga tttccagacg gccagccgca
    25081 catcacccga caccggactg cgcagctcgc gcgtgagcat ttgctcctcg ttcaccttcc
    25141 aaaacaccac gcccgccgcc atcaccgcca gcagtccgcc gccgagcagg ggccagaaga
    25201 cctgcggacg cgcgcgccac accatcagca gcgacaaccc ggcaaagacc gccccgcccg
    25261 ccgccaggct gatcaacgcg ccccggctca aagtcagcgc gcagcccgcc gccatcgcca
    25321 caatgagaaa gccggccaac gccttgagca gcgcgccgcc gcgcccgtag cacaaccagc
    25381 ccgcgctgag aaaaagcgcc atcgcaaaga aagcgccgag gtggttgggg ttggcgaaaa
    25441 agccgccgat ccggcccgcg cccgccgcgc gcacgaagtc aggcacgacg tggaaatccc
    25501 agcgcccgga gaaatgcaca aaacccgccg ccagattccc cgccgccgcg gccagcagca
    25561 gcaccagcag ccaggcgcgc ctttgcggat ggctgcacgc cgtcaccaca agaaggtaaa
    25621 ccacccagca cgacgcagcg atcaccatgt cctccagcgc ccacgccggc acaggcgaga
    25681 gccaggcgcg gaccatcaca tggacggtca gcagggacgc cgccagcagg cacgcatccg
    25741 atggggcgaa catcacccgc agccgccagc gcagcgcggc caccgcgccc gcgaacccca
    25801 gcagcagagc gcccggccag aaaaacaacg gacgcgtttc cgtggccagc acccaggaaa
    25861 ggaccaaacc caggcacatc aaaatgacag acaggctttg catcagggtt tgcctttcac
    25921 aggcggcaaa aaggtgaccc gcagccggcg caccgctccg tcgaagggcg tgctgttggg
    25981 ataatccttc acattcaaaa tgccgaagct ctggccggcg gcgatgccct tcccagggtt
    26041 gcctgggaag ccgtcagtga atgacgcgcg ctccagaacc tcgctgcggc ccggctccgc
    26101 gagggagatg aggccgtcgt aatccagcgt ggcacgcaac tggcaggggc ctgccttgag
    26161 cgggctgatg gtggctgatc cggctttgcc attgatgaaa accgtgaaca cgggcgcgct
    26221 gtccacgagg tggagaaccc agccaagttt ggcgtcgcca tgcccgacca gggtgctgtc
    26281 catgtccacc tcgggattga agtcgaaggc cgcgtccaca aagacgccgc gcccggcgag
    26341 cttcggggac tcaccggcgc tgagttcgtc cccgatctgc aactccagat gctcggagcc
    26401 gtcctggctg gggcctttga caatttcacc cgccgggcgg cgagcgctct ggaagacttt
    26461 cggaaaatgc gctgcgagct cttttttcac cgcctcatgc tccgggagcg acgccagatt
    26521 cgtccactca ttcggatcat tgtcgtgatc atacagctcc tcgccgccgt cggaatagcg
    26581 gatgtaacgc cagcgccggt cgcgtgccgc gtagctggct ttgtccgccc cgcccatggc
    26641 agtgatggcg ggccgcgcgc gctttgcgga ggggtctttg agcaggggca gcaggctctc
    26701 gccgtcgagg tgcttcggct tggcgacctt gagcaggtcg caaacggtcg ggtaaagatc
    26761 aagcagggac accgcctctc cgctgacgct gtccggcttg gtgagaccgg gcgcgtgaac
    26821 ggtcaatggc acatgcgtgg cctcctccca gagtttgcct ttgtgccaca tctgcttctc
    26881 gccgagatac cagccgtggt cggaggtgaa aatgacgatc gtgttcctcg cgttcggccc
    26941 cgactccagc gcggcgagca gctcgccgag ccgcgcgtcg caaaaagcca cgttcgccag
    27001 gtaggcctgc acggcctcgc gccatttgtc gcgcgccaga atcagcgcgt gatgaccgcc
    27061 ctgcttctca tgtcctttgg caaaagcagg cacgtcgtcg agatcgtccg cgcgcacttc
    27121 cggcagcacg gtctcgtcca gcggatgcgg gtcgaaatac ttgcgcggca cataccaggg
    27181 cgagtgaggc ttgtagaggc ccacggcgag gaaaaaaggc ttcctctgtt tctgcttcaa
    27241 aaagccgccc gcccactgcg tcaccgcgcc gtcgtcggtc agctcgtcgg gaagatccag
    27301 cgcgccccag tcccagtgcc agatgccgag gccgttgaag tcctggccga tgcggacctg
    27361 catttccggg atctgcacgc cgggcgcggg aaagcgccgc tcccaggccg cgtcctgctc
    27421 gaacccgcgc ctgccgccat gccagcccgc cagccgcgcc tcggggccgc cgtggttggc
    27481 atggaagatt tttccagccg cggcggtgag gaaacccatg gcgcgcaaat gctccggaag
    27541 cgtggttttt ccagtgatct gaacgctccg ccgccagtcc tgctcgttgc caaagacgcc
    27601 gctggaggcg ggcgccatgc cggtcatgag gctggtgcgg gagggattgc acaatgcgta
    27661 gctgcaatgc gcgttggcga acctcatgcc catgctcgcc agccggtcca tgtgcggcgt
    27721 gcgagcctgc ggatgcccgc ccagccagcc tgtgtagtcg cgcaagtcgt cggcgatgat
    27781 gaagagcacg ttcggcttgc cgtaagcgga cttgcttttc ggcgcggcgg ctgatgctgc
    27841 ctggaaagcc ggcgcgcaga gcagaagaat gagcgtccct cgaagaagca tcggttaatt
    27901 gagcatccag ctcgcggctt ggcaaggcat cggcttggcg gagatcatgg cagttgcgcg
    27961 agcattttgc cctccgggtc gaggagggtg agccggccgc cctcgggcgt gacggtggca
    28021 atgaggccgt tgctgcccgt gttgttgagc accatgctgc cggcctcgtc atacgcggcc
    28081 agggtgacac gctgcatgcc gaggtcgttg ttgatgagca tccgcccgcc gtggtccgtg
    28141 ctgtggagtg tcataaggcg cgtgccatcg ttcgagcaaa tggcaagctc gccgccgagc
    28201 ggtccgccgg agagggaggc gatgccgccc tccgcctcct tcaacccgta ggcgcagatc
    28261 acaccgccgt gctccgtcct gcccgccatc accagcggcc tgctgccgtc cgtgaccatg
    28321 agactgggag agcctttggc gacggatgcc gagatgtggc aggtctcgtc atccggtgat
    28381 gtcaccttga caaaagctgt ctcgtcattg gcaaaaagca aaacaccctc cgccttcgag
    28441 gcccccctca tgctcaggct ggaatgactg ccaggcgcgt gccgaagcgc cagcgcttca
    28501 tcctcgtcat ctttgagaaa gaccagcccc gcccctccgc ccagcgcgga aatgcccaca
    28561 cgctgcgcgc ccttttcatc gtgcagttcg acgctgctgc cgaaatcgcc ggccagcagg
    28621 ctcagtccgt cattgccgtc aggaaccttg ccgggatgca tcttgaggca ggagatctgt
    28681 tcgccgcaca tcatgctgat gctgtttttg ttttctttgc tgtgaagcat gagggagccg
    28741 ccatccggcc ccatgcccag cacagcgcca tccggctggg ctggcgtgcc ggcagccata
    28801 aatgaggacg tgccatccgt gtgcagtttc agaaccgtgg agccttgcgc ggtggcaaag
    28861 agcaaatcgg tggatgaagt cttctcctgc gctccctgcc cttcctcacc gccatcttga
    28921 tggaagagca cgccgcgcgt gcgtccgtcg ggttgcagca cggccacgga gccgccgccg
    28981 ggcagggcgc gcatcacggc acccggcgcg ccttccgccc ccagaacagc cgcgaggccg
    29041 tgatcatttt ggaccatcag ttccaaacgt gtcttccaat ccctgccaaa gacctgcaca
    29101 tgaggcacgc cctcctcgtc cacacttagc cggatggccg tgtgcatgtt gtgctccatg
    29161 ccagggtagt gcaggtcaaa atacacgccc tgctgccccg cgcccacatg cgcggcgatt
    29221 tgacgccggt catgggcggg gcgcagggtg aagtcggtgc actccatgta gacgcgctcc
    29281 acaccgtcat catcctcgtt atccaccgtc acatagcggc tgatcctggc cagccttgac
    29341 tttagctcgt ccagctccgt ccgcatggat gccaatgtgt ctttaagttg ttcaatctcc
    29401 tcgttcataa acagtggtgg gttgccttgg aatttaccag ggtgccggct tggcgcgcaa
    29461 gcgggattgc cgggtgctga gtgcttgcgc gcagccttgg acattcctgt tttccggcac
    29521 aaatatccat ccagccccct gcgcatgacc cgcccgtgcc tccatgtctt cctggccgcg
    29581 ctcttttacg ccggagctcc ggagacgcgc gctcatccgc tcatggccga gccggtcaat
    29641 catccgctcg tcttcacctt cgaccagttt tacctgaccg tggatccgga cgagcacctt
    29701 gcaggaggtg gccacctcct gctcgccgag ttgaactgct ccgcctgcca cgccgcgcct
    29761 gatgcctggc ggccttggct ggctccaaaa ccgggcccgc gcctcgacgg tgtcggctcg
    29821 cgcatggatg cggacatgct ttggctgctc atccgcagtc cgcagcattt gaaaaaaggc
    29881 acccagatgc ccggactatt ctccggcgcg gagggagacg aggaaaaagt cgaggcggtg
    29941 gtggaatttc tctccgcctc aacgtgggac gcgccgcaaa tgcccgcagg tgacgcgatg
    30001 cgcggacgca acctttatca tgaaaccggc tgcgtggcct gccatgaacc cgcgtccgac
    30061 caccgcccgg cgaatgcgcc ggaggatgcg gagctggacc gtcccggcaa cgcctccgtg
    30121 cccgtcgtgc tcgcggatgt ctggcatcag gacgcgctcg ccgccttttt gcatcagccg
    30181 ctcgccttcc ggcctgcggg tcgcatgccg gacatgctgc tcacttcgca ggaggccgcg
    30241 gacatcgccg cctatctgca cctcggccgc acccagcccg ggaatgccct tcgcgcggcc
    30301 ctgcagatcc ccccgcaggg cattgagcgc ggacgacagg tctttcatga gatgcgctgc
    30361 gccgcctgcc atgaggctcc cggttcgtca cccgtcacgg ctccgtcatc acaccccatg
    30421 cgggcgctca ggcttgatca gggctgcctc gcggagagac agacttcggg cattccccgc
    30481 tatgatttga acgacctgca aaagcgcgcc ctgcgcctgg ctctcatctc cctgcaagca
    30541 cgagcgaaac ccgatcatct ggccggtcca gcccaacaaa ccgactggca aatgacacgg
    30601 ctgaattgct atgcctgcca cgaccgcgga tacaaaggcg gccccgaaga cccgcgcgcg
    30661 cttcatttca ccggcaccgg ccttgccatc gggcagccag gaggcagcgc gcatctgcct
    30721 ccgtcactcg accaagcagg cgcgaggctc ggacgtgaag gcttggagaa aatccttctc
    30781 ggcccgcgcg ctcccgcctc gtctcacacg cgcatgccgc tctttggcgc cccccaagtc
    30841 cggcctctcg tggactggct gctggagacg gacaagggga tgcccgcgcg ctgacacgag
    30901 cggcgcgaaa ccgcccttgc cgcagcgcgc gtgacgtgcc agcatcagcg ccatgtcacc
    30961 cgcattttac cacatcgccc acgtcgtcgg tctcatcttc attttcaccg gttttggcgc
    31021 cctgctggcc gacacagcct cccgcgcggc gatgaagtgg cacggcatcg ggctggtcat
    31081 cagcctggtt tccggcttcg gtctcgtcgc caaactgcag ctgccctaca cctccacctg
    31141 gctgctggtg aaaatgggcc tctggcttgt gctcgggttt cttcctgtgc tggcgcggcg
    31201 caaggtgctg cctgcgggcg tggtggtgct gatcgccatc gtcatcgggg gcggcctggc
    31261 ttttctcggc tatcccacag gcggtcgcgt gatgctgccc tgagccgcga ggatcaaacg
    31321 accagccagc gggcgaggcc gaggaaaaaa cccatgctga cgatgtcggt ggcggtggtg
    31381 aggaagatgc tgctggcggt ggcggggtcc gcgccgaggc ggcgcaaacc aagcgggatc
    31441 atggctccgc aaaggccgct gacgatgcaa ctggcgatca tggaaatgaa gaccacaatg
    31501 ccgagcagga cggctttttc atggccctgc atcagagcgt aacccgtcat gcccaggccg
    31561 gcggtcagtc ccaccagcgc gccgttgaga aggccgagca gcccctcctt gatgacgagg
    31621 cggcgctcct gccctgagcc aatttctccc agagtcagcc cccgcaaagc gacagcgagc
    31681 gcctggcagc ctgtgttccc tgattgaccc gccatgaccg gcagaaagac cgcgagaatg
    31741 agcacctggt ccagagtgtc ctgaaaagcg ccaaccacgg ctccggcggc gaaagcggtg
    31801 agaagattca cctggagcca gggatgacgc agtttcaagc tgcgcagaag gggggtggtg
    31861 agacgctcct ccttctccac acccatcatg gttcctgcct gcgcgctgat ctcgatggcc
    31921 cgcgcctcga aaaggctctg gccgcgcacg aggccgatga ggtgacctcc ggcatcacag
    31981 acgggataaa ccggatagtg ccggttgacg gaagccttca tcgcatctgt cagggacatc
    32041 tccggactga gggtgaaaag acgcgtccac atgacctccg agagcggttt gtcagggtcg
    32101 ccgagcatga gatcgcgcat gaccaggaca ccaatcaggc ggttctgccc gtcggtgaca
    32161 taaccataag tgatgaacgc ttttttggtc agaacacgca gggcctcgat ggtctcgcgc
    32221 acggtcatct gcggacggaa gacggccacg ggcggctcca tgagccagcc gacgctgtcc
    32281 tccgggtatt cctggttgcg catccactgg cgggcttttt cgacaggcgc ggcagcgagc
    32341 acctcgcagc gcttgcgctc gtccatctcg gagagaatct gctgcgcgag catcgggttg
    32401 acctcgatga gggcggtggc tgcctccaca ggaggcactt cggaaagatg atccgccgcg
    32461 tcataaggcg cgcggcgggt gagatcgtcc gaaagcgatg aggcgttttc catcatttgg
    32521 gaaggtggga tgcgcggatg atgtcacaag tggaaaacgc gcgtcgcaca tccatccggg
    32581 gctgcgcctt tcagccgatt cgcgcaagag cgtcgctgac cgcgccctcc agagcgcgcg
    32641 ggccgtcacc gccgagtttt tcatccagcg cctcaagccg ctggatcaga tccgcgatgt
    32701 gcctgcgcgg ataaacaccg cccgcagcct ggcaaaagtg tgtgcggcag ccaaaaggcc
    32761 ggtgctggta aatggcgcag cgcccgtccc tgccgagcat gggacaggcg ccgtctggat
    32821 gcggagtcag ttttttgcgc cccgcggcgc gcacaccgcg cgcggcgagc aacgcctccc
    32881 ccagggttag catgggcgtc tttcccgtca ggcggaaatg gcagcactgc gcgcgcgcga
    32941 cacactgccg gtccagcggg cgcttttcca actccgcgta gatggcgcgc acctcgtcca
    33001 gcgcggcgct gtggtccgga gtggagcgca tgatgcggcg gatcactccg gaaggttgat
    33061 ctggtcctcc ggctgaaggg tcacatccac ggcggggttg ccgatgtcgc gcgagagatt
    33121 gtgctcggtg acgcgggcgc cgcgcacgag gcgcacgcgc ttcatgctgg caaagggcgt
    33181 gggcccgccg gcgaactgga ttgcctggga aagcgtcatg ccagggcggt aaggcacggg
    33241 cccgggtttg ttgacgccgg tgacataaac ctggcgcgcg gtctggtctg tgtctatgga
    33301 aacgagcacg ttcgggcgcg cgtaaatttc ccgcgaaacg taaatctgct cgatggagcg
    33361 ctgaagcgcg gagggcttga gaccgctggc ccgcacctcg ccgatgtgaa gcagattgat
    33421 ggtgccattg tcgctgatgg tatagacctt gttgatctgc accacctcgt tgtcgggtat
    33481 ggcgccgatg ctgatggtga tgcggtcgcc agacttcaag ggcagctcgc cctgggcgtg
    33541 gacgcgctgc gacacggcgc aaaagacaag caggaggagg aggcgtgagt tcatggcgga
    33601 agtcagaata aatccccacc tggcgtccgg gcaggcgcgg tttctccggc ggctttgcgg
    33661 cgctttttgg gcttgcctcc ctcggcggcg gggcggttgt cgggggagta gtaagtgtag
    33721 tagtttgtgt agtactggta ctggctgtcg gcccgcaggt ccacgttgtt gagcacgacg
    33781 ccgagaacgg tgccgccgac gttttccacg gtctgcttca cacgcatgag tgtctggcgc
    33841 ggcagacggc ggtgctggac gacgatcatg gtgaggtccg cctcattcgc gagcacggaa
    33901 gcgtcgctga caccgagaat gggcggggag tctatgagca cgatgtcgaa gcggctctta
    33961 gcatcctcaa tgaggtctga catgcgctgg gagttcagga tgccggcgct gtcggcgggc
    34021 aggagaccgc tgggcagaaa gtaaaggttt tccacagcgg tgcgtacgac gacgtcctcc
    34081 atgcgcacgt tggtggtgag aaagttggtg aggccagtgg tgttgccggc atcgaagtgc
    34141 gtgtgcatcg aggggcggcg aaggtcggcg tctatgagca ggacgctgta tcccccctgg
    34201 gcgcagacga cggcgaggtt gcagagcgtg gtggacttgc cctcaccggc gccgccgctg
    34261 gtgacggtga tgcagttggc atcgcggttt ttgcggttga actcgatgtt cgtgcgcagg
    34321 atgcggtagg cctcggcgtc gggattgaag cctgtggcac ggtgcaggat gccgacattt
    34381 ttcggcacca cggcaagcac aggcacgccc agaaaacgct cgacatcgtc gaggctcttg
    34441 atgctggtgt ccatgaactc caaagcgaag gcgaggccga agccgaacat gaggccaagg
    34501 atgccgccaa gggcgagatt tagcgcgaca ttgggctttg cggggcggcc ctccggctcc
    34561 gccttggcgt agattgtggc cggggatttg aaaatctcca gaccagcctt ggtctcgaaa
    34621 tatctcgtct tcagcgtgcg caggatggcc tcgatgtcgg tcacgtcgtc tttggcggtc
    34681 ttgtattcat tgaaggcgga ctgctgcccg agcagttcgg atttggcgtc ctggttggag
    34741 gcttcgagag cctccttcct tttgcgggcg ctctccagcc ggttttgcag tccagccaga
    34801 tggtctttgg cagcggcggc tatcaactcc ttgtatttgg tgatctggct gtcattggcg
    34861 agcacctccg gatgcctgcg accacggccc tgattgacaa gtccctcgcg ggcgacctca
    34921 agccgctgca attcaccggt catctgggcg acattggcgt tgtcgagctt caagccgccg
    34981 gcgcgggcaa cgagttcgtc cccttccaaa gtctgcaact ggccgatttc cgcgctgagg
    35041 gtttccacat cgagatccgc ctgaaagatg gcctgctcgc gagtaaccgc catggagtcc
    35101 ccggtggtgc gcagctcggc tcccggcacg gtggagttcg gccctgcgga gaaccactcg
    35161 ccgacgatgc cggccttggt cttggcctcc tgcattttga gctgggcctg ggcgcggagt
    35221 tgctcctgct cggtgatctg cttgttgagc atctcgagag ccgattcctt ctgtttggac
    35281 tccacctcga cacggcgtgt catgtagctg tcggcgaccg cgttggcgat ggtcgcggcg
    35341 agcgccgggt ccttgtcaaa aaactcgatg atgacgaagt cggaccggat gctggactgg
    35401 gtgtcgagat tggagcgtaa tttgccaagc gctgcctgac gggtgggcag ctcccacttc
    35461 aactgaaggt ccagcttgtc caccacaggg tagagcgttt cgggcttggt gatgctctcg
    35521 aattcatttt ttacaacgac atcgctgttt gccatgaagt tgtcgctgga gcgctcgaag
    35581 acctgcacct tgtcctgaag ccgctcgatt ttcatctcaa cccggccgcg gtatttgcgc
    35641 ggcatgatgt aggtgagcat ggcggcggag ccaaagacga ggaggaatga aagtatgatg
    35701 agtccagccc ggccacggat gacgcgccag gaatcgaggg cgtgattttg caggtcgggg
    35761 ctggcttcgc tcatgagtct ttgattatcc gtttcgcttg atcatccgcc gtttcacaaa
    35821 cagccagcgg gggcgcgagg ccgcccgctg gccgggatgc tggctgttgt ttccgggaga
    35881 gtggtttaga aagaggcggt cacaccgacg gagacattat gacggtcgaa gctgcggaag
    35941 gcgtcgtcgg agtcgagcag ggagtagctg taacgggtat cgagactgag attctccaga
    36001 atctgatagg aaagcccggt ggaaagcagc agagagtgct ccgtcacatc agtgatcgcg
    36061 ccatcaccat cgaattcgct gtaggcataa gaagcgccgg cttgaacgcc aaaacgcttg
    36121 gtgacttggt gatggaaatt cacacccgtg cgcaaggcat agcggctgtc atatgcgcca
    36181 agttcggcgc catcataccc ggccacacca aaccagcgcg cagtcgtttt gcgggcgacc
    36241 tgatggctga gcgcaatctc gccatatggc gcgcttttcc agccgccgta tgagcggaag
    36301 aactcggcac ccgcgcggaa ggatcccgtg gtgcgttcgc tccaggcatg atccagaccc
    36361 gccagcgcga aatgggattg gaagtcaggc cttccagccg ccctgaactg cgtgtggcgg
    36421 tagcgatatt cggcgttcaa cgtcgtgcgc tggctgatgg catagctgaa ctgctgcgcc
    36481 acaagatggg acagacggtc ctcgaaacta gaaacgagat tgtcgtcata agtgattccg
    36541 tccacggtgt atgacgtggt ggtggaaaag cgctgggacc aggcgtagct gacgttgaag
    36601 ttgttgtagc cgtaaaggta ctgcccgttg aagagcgtgg tcgagcctcc gaaagcgagg
    36661 ttgggctccg cctcgtaggt cagataaaaa ttattgctga tcttgagccg ctcggacagc
    36721 ttgtgagcca ggttgaaagc gacacgggcg ttgtaaaacg tgtcgtcata acgaggcatg
    36781 tcatcaaggt agtgtgtggc gccaaggtcc agatccatgc tccagggagt gactggatcc
    36841 gcctccgtgt aggtcatgcc gaccccaccc tggatgtacc acgattcgaa atctggcagg
    36901 aaggccgagg gaacgtcata cttgaggttg tcatagccgc cgcgtgtggt gatgctccag
    36961 gtcaggggct gatcctcggt gaagtcgccg gagaaatttc tcaccgcagt caacccttgg
    37021 gaataaccag tgactgtcgt ggccgccatc atgatggcgc ataacgatgg gagacagtgg
    37081 cgcatgatga cgggggtgta gattcgtcgt atttcagtga gcccggagca tcaattaaat
    37141 ccgggctggt tttggcttgg cgagagcgga tcgtcacaat ctttccggca acgcggatca
    37201 cgcgggatga atccggacgc gggcggctgg atcaaataaa cgccgcggat gctggcgggc
    37261 acgacaggtt caaaaacagg ctccggagcc actggaagcg ggtttttagc agggttcttc
    37321 gccgaagtgt tgagatcttg cggcgcggcc acaaactggg cggcgatggc ggttccggga
    37381 gaaactgccg tggcgctgtc tgtgatgacc gaggccaatt tgggggcgac cgagatgccc
    37441 gtttgcacaa tttgattgac cagggccgca tccgctttgg aggcaataat ggcagccttg
    37501 atgatctcgc cggcgcagga ctcgttgatc acaagcgcgt cctcgacaat catcagaacc
    37561 ttggaaggat cattgctgac agcggccgtg acatcgctcg aaatgttcgc gcaggaactg
    37621 ttttgtccgt gcgtcggcat ggtgccaagc aaggcggcgg tgaacgtcaa accgaagaaa
    37681 cggaatgcgg aaagggcgct cataatggct tgaggatgag attgttaatt cgctatgact
    37741 ggagcgggta gtgggaatcg aacccacatg gccagcgtgg aaggctggaa ctttaccatt
    37801 aagctatacc cgcaattaaa gccatagtaa cactcaatcc tcgcaaacag caaggtattt
    37861 ttataattta aacaaaaaat tttaaaaatt cagaaaaatt gatggattca acatcctgat
    37921 tgtgtgccac acctgtcttt cagcagaatt cgaatgtcta gccaccttcg agggccatgc
    37981 aagagttgat ttttgagatc ttgaggcact ctcaattgcg tcaagccgtg gttcagtgcg
    38041 caagattttt cagaaatttt attgacggtc agtgtgaaat tgggttcaac gaatatgtcc
    38101 aatttcaaaa tcagcgcttg ttcgacgggc attgtccagg cgtccggctt tgagtttggc
    38161 cgaaaagttg cttcaaaaac aaggagaatt cttgaaaccc tgtgatagaa tgaccacccc
    38221 ctgaatttca tcaggaaatc aagaatccca aatctgcatc gtaccaaccc t
//
LOCUS       DAOMYJ010000024        38146 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Prosthecobacter sp. isolate AMR_MDS_5629
            NODE_406_length_38146_cov_22.047912, whole genome shotgun sequence.
ACCESSION   DAOMYJ010000024 DAOMYJ010000000
VERSION     DAOMYJ010000024.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867850
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prosthecobacter sp. (wastewater metagenome)
  ORGANISM  Prosthecobacter sp.
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Verrucomicrobiaceae; Prosthecobacter.
REFERENCE   1  (bases 1 to 38146)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 38146)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.30
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:34:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,742
            CDSs (total)                      :: 4,684
            Genes (coding)                    :: 4,676
            CDSs (with protein)               :: 4,676
            Genes (RNA)                       :: 58
            tRNAs                             :: 55
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..38146
                     /organism="Prosthecobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_406_length_38146_cov_22.047912"
                     /isolate="AMR_MDS_5629"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1965333"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1049
                     /gene="eboE"
                     /locus_tag="PLP58_04285"
     CDS             <1..1049
                     /gene="eboE"
                     /locus_tag="PLP58_04285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009960062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="metabolite traffic protein EboE"
                     /protein_id="HRJ07761.1"
                     /translation="GIGLRLGADAARELALPARLEEFKKWLEANDCYVFTINGFPYGA
                     FHGTRVKEQVFRPDWTAPERLEYTKLLFDILARLLPPGVPGSVSTLPGSHKTFGIGAD
                     ELDAIFTNLRLCREHIEKTAAATGHDLHLGLEPEPLGLFETSGETLKFFGLYHDRHPQ
                     DRDFFKFIGLNYDTCHLAIEYEDADRALDRLTGAGIRLSKLHFSSALSLVPTPENLAR
                     LAAFDEPVYFHQVIASHGDTEPLRRFKDLPDALQHAARHPGDLGQEWRVHFHIPLHAE
                     PGGGFGSTRAHLLGAMDWLGRHPAACSHIEMETYTWEVLPPELHSGDVADQLVKEYEW
                     TLGEMRARGLRPLE"
     gene            1086..1517
                     /locus_tag="PLP58_04290"
     CDS             1086..1517
                     /locus_tag="PLP58_04290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ07762.1"
                     /translation="MTESELLEKLRKIERLHAGAATPGERDAAADALERIRRRLGEVQ
                     RVEKAIEFKFTLADGWSKRLFIALLRRYSLKPYRYARQRRNTVMVRVPRSFVNETLWP
                     EFLELSKVLESYLEDVTRRVIAQAVHADTAEEEIIQGDYLE"
     gene            complement(2080..2952)
                     /gene="galU"
                     /locus_tag="PLP58_04295"
     CDS             complement(2080..2952)
                     /gene="galU"
                     /locus_tag="PLP58_04295"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009964018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003983 - UTP:glucose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009225 - nucleotide-sugar metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HRJ07763.1"
                     /translation="MFQKVRKAVIPAAGYGTRFLPVAKAVPKEMLPLVDKPVIQYVVE
                     EAAASGITDILIVISRSKRAIEEHFHPAHDLESELEAKGRAAELDSLRALQNLARIHF
                     VWQARMGGLGDAVLHAREHVGDEPFAVLLGDTVVTTRGSPLTRQLADVVERHGGSAVA
                     LQEVPVEKVSRYGILGGSEVEPGLLRAETFVEKPAPESAPSRLAVSARYVLSPRIFAH
                     LEKTPKGKGGELQLTDAMASLMKEEALHGLRYEGERHDIGNKLDFLKANIRFGLERPD
                     IGPDLRAFLRGLGV"
     gene            3107..4303
                     /locus_tag="PLP58_04300"
     CDS             3107..4303
                     /locus_tag="PLP58_04300"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009965887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialidase family protein"
                     /protein_id="HRJ07764.1"
                     /translation="MKHLPALLALISCPLTAAEPFLEKLDLFKAGDDPAYNIYHIPGV
                     VVTAAGTVLAWCEARKRPAGVSDWDDIRILLRRSTDEGRTWSAPRSIADVAGPKRKNP
                     FALRMKNVDPNDVTYNNPVLIADRDGAVHMLFCLEYERVFYQRSADDGVSWGAPVEIT
                     AAFDAFKKDYDWKVLATGPNHGIQLQSGRLVVPVWLSTGEGGNAHRPSVTATVYSDDR
                     GATWKAGEIAVPNTDEWINPNETVAVELKDGRVMLNVRNESRTHRRLVTTSADGATGW
                     STPRFDEALLEPICMAGLARYDHGGRSLLLFSNPHNLEGGRGGNPEPGKSRARKNLSV
                     KISADEGGTWTVNKTLEPGPSMYSDLAVTRQGTILCFYGRSDQPGFAGSALTLARFNL
                     EWLEQP"
     gene            complement(4304..5575)
                     /locus_tag="PLP58_04305"
     CDS             complement(4304..5575)
                     /locus_tag="PLP58_04305"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009959631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004019 - adenylosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HRJ07765.1"
                     /translation="MSNTIVVGAQWGDEGKGKIVDYLTEKTDVVVRAAGGNNAGHTVI
                     NNGVKYILHLIPSGILWEGKVCVIGNGVVMDPLGLIEEMEKLRGQGVSITPENLLISE
                     TAHLVMPYHRGLDQAREAKRGDKKIGTTGRGIGPAYADKVERGGLRAILLKQPEIFAA
                     ELRERILQHNETLRAASVAEVEVEPTVSTMLEAARVLAPHITNTAVYCHQAIRAGKNL
                     LFEGAQGTYLDIDHGTYPFVTSSNTTAGGACTGSGVPPRMIDKVVAVAKAYTTRVGSG
                     PFVTENDDIGDMLHNMGREYGATTGRARRCGWLDAVLVRYAVMINGADELAITNLDGL
                     DGLDTVQICTAYKLRGETLHYPPSTAADLEACEPVYETHQGWQRDLSGIKDFNDLPDL
                     AKAYLKRVEELTGARVSLLGVGPARDQTLVA"
     gene            complement(5691..6188)
                     /locus_tag="PLP58_04310"
     CDS             complement(5691..6188)
                     /locus_tag="PLP58_04310"
                     /inference="COORDINATES: protein motif:HMM:NF028007.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HRJ07766.1"
                     /translation="MAKIQFTTPAGESGEVELTAGFVSVGRADDNMLVIADESVSSHH
                     GQIVQENGVWVFTDLGSTNGTKVDGQRVERVELDSGGAFVLGSVECVFIGDSASPSPA
                     PSRAPARAPSGGYADMPVNKSARRGFGPKAKPQGSGSGPLMILGVIALLVCAYAAFTF
                     TGMGG"
     gene            complement(6261..8627)
                     /locus_tag="PLP58_04315"
     CDS             complement(6261..8627)
                     /locus_tag="PLP58_04315"
                     /inference="COORDINATES: protein motif:HMM:NF012485.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ07767.1"
                     /translation="MLSALCATQEPCAAQTSGLASLADKETARRAELVQQQAARLLEG
                     KRLLDEGKTVESLAAFEEAWQTLPDVPMAKEFRALALDGYVTAGCRRAGDLVKAGDYP
                     AARTLLDKLDSAAAVHGDARIRKARELMNDPDRHPPALTPAHISKVEQVSKLLVLANS
                     QVETGQFNAALQSYEDVLRLDPYNTAARRGMERVEQERARYFESARDHGRAKMLNDVN
                     RAWEAEVPVKNLSGMFGGQEGAAGAQQAGTRNGREAIQKKLRELIISQVDFSEASLEE
                     VLEYLRVRTRDLDPAGKGVDFVVSVPADHPARPVSLNLREVPVEEVLRYVADMAGVTY
                     KVDNFAVRLVSLADDSAEIISKSYRVPPDFISSSPVGAGADPAGSNPDPFAAGNTQTA
                     GAPQLRRIGAKEFLEGQGVSFPEGSGASFNPSSSTLIVRNTAKNMEMVDMLVEQAFNR
                     SPRQVVIEVKLLEVANNRLEELGFDWLLGGFGGEVVGAGGTVGNAQQANYLTGDFPQQ
                     VATAAGTTALGFNPVTAGLRSSGDLPTNTVDSVLSGVVTPTSRRSPGVLSLSGVMTSP
                     QFQGVMRALDQKKGVDLAAQPSVVTRSGQKASIEITRELIYPTEFDPPQVPTSVGQFQ
                     LGNVIIITPPPPPVVTPTTPTAFDMRRTGVVLEVEPVISEDGRSVDLAVTPEFTEFVG
                     FVNYGTPIFGAFQGVRNMLTENFIMQPIFETKKVVTAVNVHDGATVVLGGLVSDNNVI
                     IQDKVPLLGDAPYVGRMFKSEVKQRQVKHVVFFVTVKVVDPSGQRVNQ"
     gene            8855..9241
                     /locus_tag="PLP58_04320"
     CDS             8855..9241
                     /locus_tag="PLP58_04320"
                     /inference="COORDINATES: protein motif:HMM:NF012790.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HRJ07768.1"
                     /translation="MKKLISLLSVLALSSLLPLAAADKAKDVPVSEAEKLVKEGRVLV
                     LDVRTPDEFKEGHIEGAVNIDFNGKDFTAKVKALDTAKPVLVHCQGGGRSGRSLPVLE
                     QLGFPAIYHLNEGFGEWEAAGKPVKK"
     gene            9389..11122
                     /locus_tag="PLP58_04325"
     CDS             9389..11122
                     /locus_tag="PLP58_04325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009964439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HRJ07769.1"
                     /translation="MPAPAAADTPAAPPPVDLVNVQIDGVWHKFPKGTRMIEACKQAK
                     KDVPHYCYHPKLTSPGNCRMCLVEMGMPPRPQPGQEPEKDANGMPKISWMPRAVIACA
                     NTVAEGMGIRTDSLLTQECRKGVMEFLLINHPLDCPICDQAGECRLQEFSVEHGKGES
                     RFREDKVKKPKNVDIGPRVRLDDERCIMCSRCIRFTAEIADDPVLGFTERGSHTTLAV
                     HPGRRLENNYSLNTVDICPVGALTSTDFRFQQRVWFLSETESVCTKCGRGCNMEIGAR
                     GDTLYRQTPRENNDVNSTWMCDQGRLDFHFVNSEFRLTEPLIKTGGKHESASWKKAMQ
                     TIASAAPGAKGGEVVIIASARMTNEELYLVRQLARTLGTENVDVVPRTGGADAYLRAD
                     DLNPNSNGVRQILGVEPGSRLDSLRGLLASGKVRLLLALGEDLLKAGFTREEIEKAGF
                     FASLHILAHASAELAQVVLPGSSYAEKSGSMINVTGRLQRLNKAVNSPGNAGDTWEIL
                     RDLNVACGGSNGLHSIEDVFHQLAAEVPCFAGLSFAKIGSLGTPVIETGETVPILERE
                     KERKARGLIVG"
     gene            complement(11156..12667)
                     /locus_tag="PLP58_04330"
     CDS             complement(11156..12667)
                     /locus_tag="PLP58_04330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009961404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HRJ07770.1"
                     /translation="MSRPKTTATGRNMALIAAFLGWMFDGFEMGLFPLIGVPALKDLL
                     PGQEPVVITNWFSVIIATFLVGAATGGVLFGWLGDRIGRVRAMSFSIFTYAIFTGLCG
                     FATEAWHIAILRFIASLGMGGEWALGVALVNELWTKGNRVWVAGAIGAAANIGYLLVA
                     FLSLGLNSYVESWHGMSMGLGLSKEWADSLFHNSAWRFLMISGAFPALLIFFIRLFVP
                     ESEKWHEEKEAGNTSHWSNTDLYGVLTGGMAGMVIIWAWSPMGLPSAAQAGVVTLFGL
                     AAVLWGYLFPVRRYLARAALAGALGTVDRKVVIRHMLIGASLAGVALLGTWGAAQQAP
                     KWSTELAANGWLHPREYTQLATSFGAILATFFMPVVADIFGRRLTYSLTCLAAFGSAV
                     LFYKTNTEISNWFFASAFIIGGVSASFYGFFPLYLPELFPTRVRATGQGFCFNVGRII
                     AAVGALQLANLVGLFGGNGAASSANAYIALCMVYFVGVAIVWLAPETKGKPLE"
     gene            complement(12818..13414)
                     /locus_tag="PLP58_04335"
     CDS             complement(12818..13414)
                     /locus_tag="PLP58_04335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009961975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HRJ07771.1"
                     /translation="MKPRLPVLAAFAAALAGLTGTTPAQETPAVSEAKPVTIVMETSK
                     GTIELELDAAKAPGTVANFVSYVKKGHYDGVVFHRVIPDFMIQGGGFTPDMEQKATDA
                     PIQNEAKNGLKNARGSIAMARTPNPHSASSQFFINLKDNGFLDYPGGDGWGYCVFGKV
                     SAGMEVVDQIAQVPTGTKSGHANVPTEPVLIKSAKVRE"
     gene            complement(13647..15737)
                     /locus_tag="PLP58_04340"
     CDS             complement(13647..15737)
                     /locus_tag="PLP58_04340"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF015722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional serine/threonine-protein
                     kinase/formylglycine-generating enzyme family protein"
                     /protein_id="HRJ07772.1"
                     /translation="MSDPVSPDSPITCPKCGSPLADPDAKGLCHACLMAEAMRPAAEG
                     DAATIPMARSAPAQPAHQAPDLPLPEELTALLPHGMYHVEGFLGQGGMGAVYQGIHVK
                     LKRPVAIKIMHREAGRDYDFAMRFEREAQAMAKLSHPNIVGVHDYGEAGPDYLYIVME
                     LVDGADLMDVIRTGRMTQEMALTLLPQICDALQFAHDHGIVHRDIKPSNIMLTREGRV
                     KIADFGLAKHFDAESSFRTQTGTGMGTPDYAAPEQFDPNATIDHRADIYALGVMIYQM
                     ITGQLPRGAWKAPSERAPVAPQWDDIVSRAMQNDPGDRYQQAREVKTDVTSIPLAGSR
                     VRKDAAEKPQAATKPRALASAAAKSRAPLIFGLVVGAVLAVGAFFALRDSGDGRTAGP
                     PAAASGPVRRSDVKPPASPSAATKDSPFVNTLGMKFVPVPVTGGPTDKQRVLFSVWET
                     RVQDFEAFVKESGHSWAKRPDVEQRPGHAVAGVSWEDAKAFCDWLTQRERAAGRLAAG
                     ETYRLPTDHEWSCAADIGGREDAAQLPHEKDGKLADVYPWGSQWPPPANSGNYWSEEL
                     RPLLAAGKYAFISGELPGYQDGQATVGPAGAQAPDRSGLHDLGGNVWEWCEDWFDQRK
                     TSRVSRGASWDMSDRTALLSSKRNHNAPNMRVFSGFRCVLSAAPVGTSSVSPSPSLPS
                     RQRPKTPPSSTPPA"
     gene            complement(15781..16512)
                     /locus_tag="PLP58_04345"
     CDS             complement(15781..16512)
                     /locus_tag="PLP58_04345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009960433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HRJ07773.1"
                     /translation="MTPEPRPASNDASFHTTRWTRVCAAKADTEDGRKALADLCDAYY
                     EPVVAFLRCELRDADAARDMSHAFFAEMLGGGTIDHAERERGRFRSYLLGAVKHFLLR
                     QRLHASRMKRGGGVEAASLDDEEAREVADATQPAADRIFDRLWARTLLDHALDALQAE
                     CTAKGKAALHDAAKPWLAGESEHGDQFALAAQLGMNLNTLKSEIHRLKRRYRELVRQE
                     VSGTLNAGDSIEEEMAALFAALRRA"
     gene            complement(16605..19787)
                     /locus_tag="PLP58_04350"
     CDS             complement(16605..19787)
                     /locus_tag="PLP58_04350"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional serine/threonine-protein
                     kinase/formylglycine-generating enzyme family protein"
                     /protein_id="HRJ07774.1"
                     /translation="MSASSPDPACSHCGTVLPPGVPESQCPRCLMAEVMLPAPDDEAP
                     TLPVARPAPSQPVNQVPDLPLPEELTALLPGGNYHVESFLGQGGMGAVYKGMQVRLKR
                     PVAIKIMHRALGKDFDFAARFEREAQAMAKLNHPNIVSVIDYGEAGPDYLFIVMELVD
                     GADMMDVIRTGRMTQEMALTLLPQICDALQFAHDHGIVHRDIKPSNIMLTRDGRVKMA
                     DFGLAKTFDAESSFRTQTGTGMGTPDYAAPEQFDPDGHIDHRADIYALGVMIYQMITG
                     QLPRGVWKPPSQRAEVAPQWDDIVEHAMQSDPADRYQQASEVKTDVGRITPSVAVGQA
                     PRLPGPAITPAGGAPALQSKSRAPLLFGVIGTVITLAVGAFFALPDAAPKSGGFQPPL
                     NVATETHPPAAQGEATTLPNTDKSSPVKNAPAAAASPTIPAPKMWIDVTTAIRDSAGA
                     DSTEALPKGWRPTRLLPNIHVPQGDKAANLAIRIRHQGPFSFKFRHTTSTESTQWYGA
                     AINGIGQAWIELGKLDKTTLENGEREPNLRLAESLPVKGFKTTSFHESIVTALGDELA
                     IWLDGALMCKVNDARLTSGYIRFRAELETVIEKIEWGEIASTVVKSAPPEAASSPIAT
                     PALPESGWKPLFTSSDWKQTGSHGEFKDGLKHLKADQWTKPQTSPDGAIRARFQFRQD
                     TRNPSVVTRLAVGHSFYKLELSNPPTFVYLQGMSGGKAETIAKHAIPKPPQPGETVEL
                     ELRAQGDHLIGKLNGETVIDAHDARVTGAGEWGINANDGWYQSVEFLDLDKTTAVSPS
                     PSLQVSSSSVAIATKDTPFVNTLGMKFVPVPITGGPTDGKRVLFSVWETRMQDYEVFA
                     KETKRKWEKPDFEQGATHPAVNLNEEDAAAFCVWLTERERKAGQLGTDKSYRLPGDHE
                     WSCAAGIGDREDAAKPPKEKSGRISTLYPWGAQWPPPPDAGNYSGEEFRDDPQSGKGG
                     RIMLEGYNDGFAHSSPVGRFAVNPHGFYDLGGNAWEWCADSQEGCLVRGASCVDGKER
                     VMLSSWRITPPPATRQPNYGFRCVLAPAAQ"
     gene            complement(19833..20561)
                     /locus_tag="PLP58_04355"
     CDS             complement(19833..20561)
                     /locus_tag="PLP58_04355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009960433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HRJ07775.1"
                     /translation="MNPELKADSTAAPFLTTRWTRVRLAKGDSEEGRLALADLCEAYY
                     EPVVAYLRCVLRDADEARDLSHAFFAEILAGGRLGTADAGRGRFRSYLLGAVKHFVSH
                     QREAARRLKRGGGASVIGLDDEEAAGVADARQPSPDTEFDRQWAITVITRSMAALRAW
                     CEAEGRGEFFEKVGGLLSGQAAHGDQTALAAECGMTFDAFRMAVTRLKKRLRQSVQEE
                     VAGTLDDPATVQDEMAALFAALSG"
     gene            complement(20771..22318)
                     /locus_tag="PLP58_04360"
     CDS             complement(20771..22318)
                     /locus_tag="PLP58_04360"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HRJ07776.1"
                     /translation="MPDFSNRSVLLILAPCLLLAFDAAAKPNILFIYTDDHSYRTLSC
                     YEGAESWAKTPNMDRLAARGVRFTHAYIGTWCMSSRTTMLTGLHQYGVRTMRMTPPYP
                     GATYDPRQCRFWPAVFRQQGYHTAQIGKWHAGNDAGYGRDWDHQIVWNRPRHPDNAPN
                     YYHDQLLSINGGEPHVVKGYSTDNYTRWAVDYIKGANRDADKPWYLWLCYGGVHAPYT
                     PAERHLDDYPGVTVATPADIYPPRPGKPGYMQKIAAWEKGPDGEPVLSDKAIGSEVGD
                     DFERDRGRSLSKWSRQYHQAVRSLDEGIGQVLAALEESGQLRNTLVVLTADQGYAWGH
                     HGFRAKLAPYDANIRSPLIISMPGTLPEGKVCRTPVGGADLPPTFFSFAGLDLPWEMH
                     GHDLTPLLKNPDGAWPHTTIMPFTADKFGADCDVVPAPPGNHHKTGIPWYVMLASGHH
                     KYIRTLEAGEPEELYDLLSDPDELTNIAGQPGNAELLNKLRAAAAAELQRTGAKMAGS
                     LPPVRAR"
     gene            complement(22376..22678)
                     /locus_tag="PLP58_04365"
     CDS             complement(22376..22678)
                     /locus_tag="PLP58_04365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ07777.1"
                     /translation="MKIPHTYRQFPGIPHDPNMVLDTLGEDNRAFYRRTLSGEATIDS
                     TDSARRSTHTSPSNMLSNLAAVSALSNSPEARPQEFYERSPCNDREFPRHPAGVER"
     gene            complement(22862..24271)
                     /gene="pyk"
                     /locus_tag="PLP58_04370"
     CDS             complement(22862..24271)
                     /gene="pyk"
                     /locus_tag="PLP58_04370"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012419484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HRJ07778.1"
                     /translation="MRKTKILCTLGPATESAERISELISKGADIFRLNMSHASHDWVR
                     TVYGRVREAATRGSKEISVLMDLTGPSIRTGDLDQPWQLQPGDQVELRCKPEAAPTVP
                     LSVTVNYPELGKDLRPGDIIMVDGGMIQIKAAEVCSSRVLGTVLTKGEMKSRRHINLP
                     GVPVRLPPLTQKDYADLDLGVELGVDFFALSFAREPGHIQHLQLLLEQKNSKARVIAK
                     IENQQALQNIDTLVEASAGIMVARGDLGSECPIEELPIIQRQIVERCSYHGRKVIVAT
                     QMLESMIENPVPTRAEVTDIFNAVIEQVDCVMLSGETSVGRYPEKCVEVLDTVIRRIE
                     KKYPSGRFASDAPLKTNKHKTVKAAILLANSIPGSKLLVFTLRGVMAHLLAHQRPEFA
                     PIFAFTPHRHVSRTLVTARGVQPFVLPFAEGEPEAAIRDAINLLRREDLIKPGDPLVI
                     LSDALYDGVNVDAILLREA"
     gene            complement(24415..24831)
                     /gene="rplQ"
                     /locus_tag="PLP58_04375"
     CDS             complement(24415..24831)
                     /gene="rplQ"
                     /locus_tag="PLP58_04375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012420542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005762 - mitochondrial large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HRJ07779.1"
                     /translation="MKHGRKVPKLQRDASHRKALLANLTCALIEHRRIRTTLAKAKAL
                     RPYAEKLVTLGKRGDLHARRLAVGTLHSKAMVKKLFEEIAVASKDRAGGYTRITKLGQ
                     RRSDSAPMAYIEWVDAYVPATVKEEAVEPAAAEAAA"
     gene            complement(24866..25984)
                     /locus_tag="PLP58_04380"
     CDS             complement(24866..25984)
                     /locus_tag="PLP58_04380"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009960622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HRJ07780.1"
                     /translation="MSVRLARFEMPNRLVRNEATATDTYAQFVAEPFDKGYGHTIGNS
                     LRRVLLSSLEGAAITSVKIRGVEHEFSTIPGVVEDVVQIVLNLKKVRFKHHSETKEPR
                     LLSVTADKEGVVTAGDIKDDNHYEIINKDQVICTLDRKTKFECEFEVRVGRGFSSWEE
                     NKRPDTPIGVIPIDSIYSPVTRVKYAVENTRVGQNTDYDKLVLDIWTDGRISPSDALL
                     QSAAILRHHLDVFVNYDDKAIEFEAAPEAQTEENSELRKLLNMSVNEIELSVRAANCL
                     NNANITSVGQLAMKSEAEMLRYRNFGKKSLTEIKEKLSELGLSLGMKFDASLLEPSAG
                     GVSLLARSSMITDEDEDADVVDFTNLVDSNLPDVEDDE"
     gene            26200..29028
                     /locus_tag="PLP58_04385"
     CDS             26200..29028
                     /locus_tag="PLP58_04385"
                     /inference="COORDINATES: protein motif:HMM:NF019607.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-dependent sugar dehydrogenase"
                     /protein_id="HRJ07781.1"
                     /translation="MKGRFAICLCLAATAACAGAEPRAPWSGSRVKGSPEPPPPYVAA
                     PVFETLELSEVLEIVPHDGSVVVLENRGRVWCFENHPRDPGPPFLMADLKVKHPALDH
                     LYGIAFHPRWRENRQVFVTYTVGAKIADGTRLSRFLLDENDPPRLVLESEQVLLTWLS
                     GGHNGAHLQFGPDGMLYISTGDAEAPAPPDLLNTGQDNSDLLSAILRIDVDPTGPALP
                     YRIPPDNPFVGKSGVRPEIWAFGFRNPWKMSFDSRGRLWCGDVGWELWEMIHLVTRGS
                     NHGWSALEGPQPIKLETLSALAPVTAPVAAHPHTEAASITGGYVYEGKKLPALRGACI
                     YGDYETGKIWALWHDGGKVTRHEEIADTPHRIVSFGLLPDGELVYAHWDKTAAFFELV
                     PNPETGRAEEFPRRLSGTGIFQNTAAQTLAPGVYEFDIAEPMWQDGLEARRFIALPGA
                     GAVASTIQRRADGTVQRAGTQWPADSVLVKTLVNESRPVETQLLHYDGAHWQGYSYAW
                     NEAGTDAELVPAEGAQKQLHGRAHAYPARAECMRCHTPWTGHALAFCPQQLAPGAGAD
                     VLRADDALRLGLVNAEFFETSSARLAGTDDAPLEDRARAWLHANCAHCHRLHGGGSVA
                     FKANIELELDAMEMLDGAPLRGDFGLPGARIVAPGRPWASTLIARTAQGGPGHMPLIG
                     AREVDTRGLDLLWRWIESLPAPAGSGEGDTDFAAALKQALADGRRDSWNKLVNDRPDR
                     ALNLARLLDVDGTLRLAFMESTHHLDAHPNAFIAALFERFLPPGKRRERLGAAPVPAA
                     ILALKGDAARGAALLSPLGALASCLACHRLGDRGISIGPDLTHAGARLTREQLLESLL
                     QPSKSIAPEYRAHSLVTSDGEVRTCFVLNGTDPEVTVKLLTGETLTYAREGIQLTPLP
                     ASLMTEGLLQSLSAQDAADLLEYLASRK"
     gene            complement(29037..30113)
                     /gene="prfA"
                     /locus_tag="PLP58_04390"
     CDS             complement(29037..30113)
                     /gene="prfA"
                     /locus_tag="PLP58_04390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018969151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016149 - translation release factor
                     activity, codon specific [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HRJ07782.1"
                     /translation="MDYSSIITSKRARLDLLGELMGQPGFFDDPKKAGELTREHRQLT
                     GLLSDWDALQKLRTEFEESQTLARGEDDEFAAMARQELPLLEKRIAELEKAVQFAILP
                     PDPLEGRDALVEIRAGTGGDEASLFAADLVRMYSRFAETRGWKVETLESSPSDVGGFK
                     EIVMKVSGEDVFRVLKYESGVHRVQRVPATEAQGRIHTSAATVAVLPEAEEVDLDLRA
                     EDIRVEVCRSSGAGGQGVNTTDSAVQVLHLPTGMIVRCQDGRSQIKNKEKALTILRSR
                     LLEKKQQEEAEKYSAHRKSLIGSGGREEKIRTYNYPQNRVTDHRIDMTLYNLDMFMVG
                     RMEAMTQALLASDLEERLAEAGLK"
     gene            complement(30195..30440)
                     /gene="rpmE"
                     /locus_tag="PLP58_04395"
     CDS             complement(30195..30440)
                     /gene="rpmE"
                     /locus_tag="PLP58_04395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009960029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L31"
                     /protein_id="HRJ07783.1"
                     /translation="MKEQGHPTTYETTITCTCGAVYPTISTVQNLRVGICAACHPFFT
                     GEQRFIDTAGRVDKFAQRYGSTRARRSAPKVSEAAAS"
     gene            30629..33547
                     /locus_tag="PLP58_04400"
     CDS             30629..33547
                     /locus_tag="PLP58_04400"
                     /inference="COORDINATES: protein motif:HMM:NF019223.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1553 domain-containing protein"
                     /protein_id="HRJ07784.1"
                     /translation="MFRASPALLLAAIPLYAAPDFEREVAPILEASCLSCHDAANAKG
                     DVRLDTQAAVLEHLKPGLPDASLLIEQVSGPEPDMPKKEKPLTPAQVETLRQWVQSGA
                     VWSEGRVLADKPARDLDWWSLREIRKPKPEARNGNPVDSFIEAKLAEKGLKPVPEADP
                     ATLIRRLSYDLTGLPPSAHNFEFRASSFDFDSLVDRLLSSPAFGEKWAQHWLDLVRYA
                     ETHGYDKDKPRMNAWPYRDYVIRSLNDDKPYDRFVQEQIAGDALFPGQPDGVLGLGFL
                     AAGPWDYIGHSEVGEGKVDGRIAKHLDRDEMVSAVFNIFTSTTVQCAQCHHHKFDPVT
                     MTDYYRLHAVLAAVDRADRVFTGLPPEKEKERNTLLARINALKAEQTKLTTDAKRELG
                     ARTSGIERRLAELKQKHGSPLRPQYGWHSHISKIQAAEKWVQVDLGKAQGVEQVRLIP
                     AYDDYNSIGAGFGFPLRYKVEAGNDPGFNTGVRLIHDATAADQPNPRNSEIVLDVGRD
                     AVRYLRVTATRLAPRKNDYIFALGEMQAIGGENHDNMAQGAAVTALDSIEAAPRWGRA
                     NLTDGIYHREVGDPAALVEMRELEVQKAAIEKELADPRRDARLDEIEKHLADLNPKLA
                     TYPKGELVYAASTDFTAQGRFIATAGKPREIRLLNRGDIRAPGGLMQPGLPPLWPGAP
                     EQFNLPTEAPESAARAELAKAITARDNPLFWRSIVNRLWLWTFGQGLVTSANDFGRMG
                     QPPTHPELLDHLAATLRDDPQHSLKNLVRLLVTSRAYRRASTHHEANAALDASNACLW
                     RANRRRLSAEEYRDSLLAVSGVLRMEDRGGPSFQDFVIEKPQHSPHYQYHLHDPRDPS
                     AHRRSIYRFIVRSQPQPFLTSLDCADPSQSVPQRDESTTALQALTQWNNRLVEAMSDT
                     FGQRLGQEKDPVNAAFTRALSRPPTAEERTVLTAHLQAHGPAALARVLYNLNAFVYLD
                     "
     gene            33552..34988
                     /locus_tag="PLP58_04405"
     CDS             33552..34988
                     /locus_tag="PLP58_04405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009958243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1501 domain-containing protein"
                     /protein_id="HRJ07785.1"
                     /translation="MNRRQFLTSLTGLALTDLLLRDAALGAPGTLRGGLHHPPKAKRV
                     IQLFMAGAASHVDMWDHKPLLTQKDGQPWDPGEKVELFQSTPGACFGSPWAFKPHGQS
                     GKMLSEIVAPLGGVVDDIAFVHNVVGKTGVHSQGTLLQTTGFQLPGFPSAGSWVSYAL
                     GSESDNLPSFVVLPDHRGLASNGAKNWASAFLPAQHQGTAIRPGSAEPVADLLPPQSE
                     FITPGSDRAGLDVLAKLNTAHAQARPGDDRLLARVRSYELAAAMQLSATDALDVSKEP
                     RHIQDLYGLASEGPGVDDTTINVKAETEFFGRKCLVARRLLERGVRFVQIWSGNDNGF
                     PRRNWDSHEDVKRDHEPLSLGMAHGTAALIKDLKQRGMLEDTIILWTTEFGRMPCSQG
                     SKGRDHNPFVFTNWLCGGGILGGTYGESDEWGFRPQDTANATTAHDIHATLLHQLGIN
                     HERLTVRHNSIDRRLTDVHGHVLKAMLV"
     gene            35040..35321
                     /locus_tag="PLP58_04410"
     CDS             35040..35321
                     /locus_tag="PLP58_04410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ07786.1"
                     /translation="MSQPANPSPHQPPAAGASAARLLALAETGRPDSYYRLARMVARV
                     LTSQSLRRRRKWCELAHALAASSGVEARLIMQALRRFDPGAAANFPVKS"
     gene            35318..36040
                     /locus_tag="PLP58_04415"
     CDS             35318..36040
                     /locus_tag="PLP58_04415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ07787.1"
                     /translation="MSWETPPPVWDTPAGRMLDAFLAALKKDAPGYSEPLTIFGSAAI
                     QLCLDESFTSADVDVMALTGNERLREIAKTAGLGATGSIRAAYGIQICPPQLFRTTPH
                     YLLRARMERRHGIKVILPHLRDVLLAKLHRSRAPDQEGLAPKDRRAFQRVRELCGGHP
                     TEADVIEDLRLCSQDLLIQAAEEPNAFRLNVLDLFRSVFGRGLDIENEILKPARQALG
                     TVQDVDHEEIQRRLASLRPDRD"
     gene            complement(36051..36962)
                     /locus_tag="PLP58_04420"
     CDS             complement(36051..36962)
                     /locus_tag="PLP58_04420"
                     /inference="COORDINATES: protein motif:HMM:NF015431.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HRJ07788.1"
                     /translation="MELRLLRSFIAAAEDGNISKAAARLSLTQPALSRQIKSLEDDLG
                     VPLLERGAHSFSLTASGELLLREGRALLERADHLEQRIKAAARAQTLRVGYSPSLTAG
                     ILAPAMEAFSQLHPKARVELSDLSSTEMVEGLQKGALDVIVTVPPGKEVPEMVWTPVQ
                     KQRWCVAVPRQHALAGKTTLLPADLDGQRLVVYNQREYPDYWALLSGWFKSQGIKARV
                     ASECDGVTSLISAVEAGLGVALVVERIACLVPERIVLKTLQPQPAPVCISAGVTTRQT
                     RDKVLAVFVEELKRAGEVFASGSHHGE"
     gene            complement(36969..37199)
                     /locus_tag="PLP58_04425"
     CDS             complement(36969..37199)
                     /locus_tag="PLP58_04425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ07789.1"
                     /translation="MQPRTVINLDEPIGKTVGRRKPPVSPAGMQETRNQFFASQGHFL
                     CPRGVYRFKTHREKDIPDLIFLRQQYPEVVTG"
     gene            complement(37285..>38146)
                     /locus_tag="PLP58_04430"
     CDS             complement(37285..>38146)
                     /locus_tag="PLP58_04430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009960982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HRJ07790.1"
                     /translation="LSETHTHGPDTDYRVVRADEADRRPWLRGAPVCGSLRAHRISHA
                     GVMTARAPYRIVRMHQGGLYFLACLEGEGRVLVDGRWQKCGSGMAVALPPFMVNAFEA
                     VKGKAWRCCWVRYEQEPGQRPILSSSSPQMAAFHGRPLWSAIEGLIEEAAHEAKPAAM
                     FHWVELIQNYVERFAQPWQQDDRLWRVWEKVAADVGRDWTLDELAHQAHVSSEHLRRL
                     CRKTLGRTPMHHVTWLRMRKACELLSTTNLKVETVSHTVGYQNPFVFSNTFKKWIGWR
                     PSEHRSRMGS"
BASE COUNT         6515 a        12463 c        12323 g         6845 t
ORIGIN      
        1 acggcatcgg cctgcgcctc ggcgcggacg ccgcgcgcga gctggcgctg cccgcgcgct
       61 tggaggagtt taaaaaatgg ctggaggcca atgactgcta cgtcttcacc atcaacggct
      121 tcccctatgg ggccttccac ggcacccgcg tcaaggagca ggtcttccgg ccggactgga
      181 ccgcccccga gcggctcgaa tacaccaagc tgctcttcga catcctggcc cgcctcctcc
      241 cccccggcgt gcccggcagc gtcagcaccc tgcccggctc ccacaagacc ttcggcatcg
      301 gcgcggacga gctcgacgcc atcttcacca acctccgcct ctgccgcgag cacatcgaaa
      361 aaaccgccgc cgccaccggg cacgatctcc acctcggcct cgaacccgag ccactcggcc
      421 tctttgagac cagcggcgag acattaaaat tcttcggcct ctaccacgac cgccatccgc
      481 aggaccgtga ctttttcaaa ttcatcggcc tgaactacga cacctgccac ctcgccatcg
      541 aatacgagga cgccgaccgc gcgctcgacc gcctcaccgg cgccggcatc cgcctcagca
      601 agctccactt cagctccgcc ctcagcctcg tccccacccc ggaaaacctg gcccgcctcg
      661 ccgccttcga cgagcccgtc tatttccacc aggtcatcgc cagccacggc gacaccgagc
      721 ccctccgccg cttcaaagac ctgccggacg cgctccagca cgccgcccgc caccccggcg
      781 acctcggcca ggaatggcgc gtgcatttcc acatccccct ccacgccgag cctggcggcg
      841 gctttggcag cacccgcgcc cacctgctcg gcgccatgga ctggctcggc cgccacccag
      901 ccgcctgctc ccacatcgag atggagacct acacctggga ggtcctgccc cccgagctgc
      961 acagcggcga cgtggccgac cagctcgtga aggaatacga gtggaccctg ggcgagatgc
     1021 gcgctcgcgg cctccggccc ctggagtgaa aaaaacgcca tcctcgctcc cgatcccgcc
     1081 ctcccatgac cgaatccgaa cttctcgaaa agctgcgcaa aatcgagcgc ctccatgccg
     1141 gcgccgccac ccccggagag cgcgatgccg ccgccgacgc cctcgagcgc atccgccgcc
     1201 gcctcggcga ggtgcagcgg gtggaaaaag ccatcgagtt caagttcacc ctcgccgacg
     1261 gctggtccaa gcgcctcttc atcgccctcc tgcgccgtta ctccctcaag ccctaccgtt
     1321 acgcccgcca gcggcggaac accgtcatgg tccgcgtccc ccgcagcttc gtgaacgaga
     1381 ccctgtggcc ggagttcctg gagttgagca aggtgctgga gagctacctc gaggacgtta
     1441 cccgccgcgt catcgcccag gccgtccacg cagacaccgc cgaggaggag atcatccagg
     1501 gcgactacct ggagtgaggg cgcggggcgg aatgcgggat ttgctccggt ttttcgggag
     1561 tctcttatgg tgaatatgaa agatttggca ttatcgcttt aaatgtgatt attcagacaa
     1621 aaaccaccaa aatgatcatt atcttggtgg ttcttgcctt caaaagggtg gttttggccg
     1681 ctgtctttat ccttttgaag gcaaggagta tctggttaac gatgaacctg ataccttttg
     1741 ccttcaaaag gatacctttg gccgctgtct tgataccttt ggtggctgtc ttgatacctt
     1801 tgcaggccgg aatgatgctt tcggcggtcg tcttgagggg actcccgaaa attcaaagca
     1861 tggggcgcag gcatcctgcc tgcgcgaaca ggcggaacgc ctgttcccca tgcaaatgcg
     1921 ggttttcggg agtgcccttt ttccattttg gcaccgccaa acccgtttgg aggcgcctcc
     1981 ttgccccaat gatctccaaa tcaattcgcg gagtcgtcag gcgggccgga agggggctgg
     2041 gattgccacg gtgacgctcc gcccgcgacc actctgccct cacaccccca gcccgcgcag
     2101 gaaggcgcgc aggtcggggc cgatgtcggg gcgctcgagt ccaaagcgga tattggcttt
     2161 gaggaagtcg agcttgttgc cgatgtcgtg ccgctcgcct tcatagcgca ggccgtggag
     2221 ggcttcttct ttcatgaggg aggccatggc gtcggtgagc tggagttcgc cgcctttgcc
     2281 cttcggggtt ttttccagat gcgcgaagat gcgcggggag agcacatagc gggcgctgac
     2341 ggcgaggcgg ctgggggcgg attcgggggc gggtttttcc acaaaggtct cggcgcgcag
     2401 caggccgggc tccacttccg agccgccgag gatgccgtag cggctgactt tttccacggg
     2461 cacctcctga agcgccacgg cgctgccgcc atgccgctcc accacatcgg ctagctggcg
     2521 ggtcagcggc gatccgcgcg tggtgacgac ggtgtccccc agcagcacgg cgaagggctc
     2581 gtcccccaca tgctcgcgcg cgtgcagcac ggcatccccc aggccgccca tgcgggcctg
     2641 ccagacgaag tggatgcggg ccaggttctg caaggcgcgc agggagtcca gctcggcggc
     2701 gcggcctttg gcctccagct cgctttccag gtcatgcgcc gggtggaagt gctcctcgat
     2761 ggcgcgcttg ctccggctga tgacgatgag gatgtcggtg atgccggagg cggcggcctc
     2821 ctcgacgacg tattggatga cgggcttgtc caccaggggc agcatttcct tgggcacggc
     2881 cttggccacg ggcaggaaac gggtgccgta accggcggcg gggatgacgg ctttgcgcac
     2941 tttctggaac atgcgccgga gcctagcggg cgcgcccgcc gggcgcaaga ctccagagcg
     3001 ggcggcaagc cggagggccg cgcgcacgca ctggtcatgc gcgtaaacca cggaggaagc
     3061 gcctttttgc ggcgttccca gtgatccaac gaagacactc ccagccatga aacacctccc
     3121 tgccctcctc gcgctcattt cctgtccgct cacagcagcg gaacctttcc tggaaaagct
     3181 ggaccttttc aaggcgggcg atgaccccgc ctacaacatc taccacatcc ccggcgtggt
     3241 ggtgacggcg gcgggcactg tgctggcctg gtgcgaggcg cgcaagcggc ccgcgggtgt
     3301 gagcgactgg gatgacatcc gcatcctgct gcgccgcagc acggatgaag ggcgcacgtg
     3361 gagcgcgccg cgcagcatcg cggatgtcgc ggggccgaag cggaagaatc ccttcgcgct
     3421 gcggatgaaa aacgtggacc ccaatgacgt gacctacaac aacccggtgc tcatcgcgga
     3481 ccgggacggc gcggtccaca tgctcttctg cctggagtat gagcgcgtct tttaccagcg
     3541 cagcgcggac gacggcgtgt catggggcgc gccggtggag atcacggcgg cgttcgacgc
     3601 cttcaaaaag gactacgact ggaaggtgct ggccacgggg ccgaaccacg gcatccagtt
     3661 gcaaagcggg cgcctcgtgg tgccggtgtg gctctccacc ggggagggcg gaaacgcgca
     3721 ccggcccagc gtgacggcga cggtttacag cgacgaccgg ggcgcgacgt ggaaggcggg
     3781 tgagatcgcg gtgccgaaca cggacgagtg gatcaacccg aacgagacgg tggccgtgga
     3841 gttgaaggat gggcgcgtga tgctcaacgt gcgcaacgag tccaggacgc accgccgcct
     3901 ggtcaccacc agcgccgatg gcgccactgg ctggagcacg ccgcgctttg acgaggcgct
     3961 gctggagccg atctgcatgg cgggcctcgc gcgctacgac cacggcggca gaagcctgct
     4021 gcttttctcc aatccccaca acctggaagg cgggcgcggc ggcaatcccg agccgggcaa
     4081 gagccgcgcg cgcaaaaacc tgagcgtgaa gatcagcgcc gacgagggcg ggacatggac
     4141 ggtcaacaaa actctggagc cgggaccaag catgtactcg gacctggccg tgacgcggca
     4201 gggaacgatc ctctgcttct atggccgctc tgaccagccc ggcttcgccg gctcggctct
     4261 gacattggcg cgcttcaacc tggagtggct ggagcagccg tgatcaggcc accagggtct
     4321 ggtcgcgcgc ggggccgacg ccgagcaggc tgacgcgcgc gccagtgagc tcctcgacgc
     4381 gcttgaggta ggctttggcc aggtcgggca ggtcgttgaa gtccttgatg ccgctgaggt
     4441 cccgctgcca gccctggtgt gtctcataca cgggttcgca ggcctccaga tcggcggcgg
     4501 tgctgggcgg gtaatgcagg gtctcgccgc gcagcttgta ggcggtgcag atttgcacgg
     4561 tgtccaggcc gtcgagcccg tcgaggttgg tgatggcgag ttcgtcggcg ccgttgatca
     4621 tcacggcata gcggacaagg acggcgtcga gccagccgca gcggcgggcg cggccggtgg
     4681 tggcgccgta ttcgcggccc atgttgtgga gcatgtcgcc gatgtcgtcg ttttcagtga
     4741 cgaaggggcc ggatccgacc cgggtggtgt aggccttggc cacggcgacc accttgtcaa
     4801 tcatgcgcgg cggcacgccg gagccggtgc aggcgccgcc ggcggtggtg ttggaggagg
     4861 tgacaaaggg gtaagtgccg tggtcaatgt cgagataggt gccctgggct ccctcaaaga
     4921 gcaggttttt cccggcgcgg atggcctggt ggcaatagac ggcggtgttg gtgatgtgcg
     4981 gggccaggac gcgcgcggcc tccagcatgg tgctgacggt gggctcgact tcgacctcgg
     5041 cgacgctggc ggcgcgcagg gtctcattgt gctggaggat gcgctcgcgc agctcggcgg
     5101 cgaagatttc cggctgcttg agcaaaatgg cgcgcagtcc gccgcgctcg accttgtcgg
     5161 cgtaggcggg gccgatgccg cggccggtgg tgccgatttt tttgtcgccg cgcttcgcct
     5221 cgcgcgcctg gtcaaggccg cgatggtagg gcatgacgag gtgggcggtt tcgctgatga
     5281 gcaggttctc cggggtgatg ctgacgccct ggccgcgcag cttttccatt tcctcgatga
     5341 ggccgagcgg gtccatgacg acgccgttgc cgatgacgca gactttcccc tcccacagga
     5401 tgccgctggg gatgaggtgg aggatgtatt tgacgccgtt gttgatgacg gtgtgaccgg
     5461 cgttgttgcc cccggcggcg cgcacgacga cgtccgtttt ctccgtgagg tagtctacga
     5521 tttttccttt tccttcatcg ccccactggg cgccgactac gatggtgttt gacatggtga
     5581 atgatgacgc ggcgcgtgtg tccgcagggg tttgggtgaa agacgggata cgggacgaat
     5641 ggcggcagct cccgccgccg ggcagtccgg ggttccctgc cggcgggtgc tcagcctccc
     5701 atgccggtga aggtgaaggc cgcgtaggcg cagaccagga gggcgatgac gccgaggatc
     5761 atcagcggcc cggagccgct gccttggggt ttggccttgg ggccgaagcc tcgccgggcg
     5821 gatttgttca ccggcatatc cgcgtagccg ccggaaggcg cgcgggcggg cgctctggaa
     5881 ggagcggggg agggactggc gctgtcgccg atgaaaacgc actcgacgct gccgagaacg
     5941 aaggcgccgc cggagtcgag ctcgacacgc tccacgcgct ggccgtccac tttggtgccg
     6001 ttggtggagc cgaggtcggt gaacacccag acgccgtttt cctggacgat ctgcccgtga
     6061 tggctggaga cggactcgtc tgcgatgacc agcatgttgt catccgcgcg gcccacggag
     6121 acgaacccgg cggtcaactc gacttcgccg ctttcgcccg ccggggtggt gaactgtatt
     6181 tttgccatag gagggccaag atttagcgcg gcgccgggga ctcgcaaggc gcaatctccc
     6241 cctcgcgcaa acctgtgaaa tcattgattc acacgctgcc ccgaggggtc caccaccttc
     6301 acggtgacga agaagacgac atgcttcacc tggcgctgct tgacctcgct tttgaacatc
     6361 ctgccgacgt agggagcgtc tccgagcagc ggcactttgt cctggatgat gacattgttg
     6421 tcagagacca gcccgcccag caccaccgtg gcgccgtcgt ggacattgac ggcggtgacg
     6481 actttcttgg tctcgaaaat gggctgcatg atgaagttct ccgtcagcat gttccggaca
     6541 ccctggaagg ctccaaaaat gggcgttcca taattcacaa agccgacaaa ttcggtaaat
     6601 tcaggcgtga cggccaaatc cacagacctg ccgtcttcgg agattacggg ttccacctca
     6661 agcaccacac cagtacgacg catgtcgaag gcggtgggcg tggtgggagt gactaccgga
     6721 ggcggaggag gtgtgatgat gatgacgttt ccaagctgaa attgcccgac acttgttggc
     6781 acctgcggtg ggtcaaattc ggtgggataa atcaattcgc gggtgatttc gatgctggcc
     6841 ttctggccgc tgcgcgtgac gacactgggt tgcgcggcga ggtccacgcc tttcttttga
     6901 tccagcgcgc gcatgactcc ctgaaattgc ggagaggtca tcacgccgct cagcgagagc
     6961 acacccggcg agcgcctgga tgtgggcgtc accactccag acaggaccga gtccaccgtg
     7021 ttggtcggca ggtcacccga ggagcgcaga cctgctgtga cggggttgaa tccaagagca
     7081 gtggtgcctg ccgctgtggc cacctgctgg gggaagtcac cggtgagata gtttgcctgc
     7141 tgggcattgc ccacagtgcc gcccgccccc acgacctcgc cgccgaagcc gccgagcagc
     7201 cagtcgaagc cgagctcctc caggcggttg ttggccactt cgagcagttt gacttctatc
     7261 accacctgcc tgggggagcg gttgaaggcc tgctcgacga gcatgtccac catctccatg
     7321 tttttggccg tgttgcgcac gatgagcgtg ctgctggagg ggttgaagct ggctccactg
     7381 ccctcaggga agctcacacc ttgtccctcc aggaattctt tggccccgat gcgccgcaat
     7441 tgcggcgcgc ctgctgtctg ggtgttgcca gccgcaaagg gatctggatt tgacccggca
     7501 gggtccgctc cagcgccgac gggcgagctg gagatgaaat ccggcggcac ccggtaggat
     7561 ttgctgatga tctctgccga gtcatccgcg agcgagacca gccgcacggc gaaattgtcc
     7621 accttgtagg tgactcctgc catgtcggcg acatagcgca ggacttcctc caccggcacc
     7681 tcccgcagat tcagggagac aggacgcgcc gggtggtcgg cgggcacgct gacgacgaaa
     7741 tccacgcctt tgcccgccgg gtcgagatcg cgggtgcgca cccgcaggta ttccagcacc
     7801 tcctccagtg aagcctcgct gaaatccact tgggagatga tcaactcgcg cagtttcttt
     7861 tgaatggcct cgcgcccgtt cctggtaccc gcctgctgcg cgccggccgc gccttcctgg
     7921 cccccgaaca tgccggacag gtttttcacg ggcacttccg cctcccaggc ccggttcaca
     7981 tcgttgagca tcttggcccg gccatggtcc cgggcgcttt caaaatagcg cgcgcgctcc
     8041 tgctccaccc gctccatgcc gcgccgggcg gcggtgttgt aggggtccag ccgcagcaca
     8101 tcctcataac tctgcagcgc cgcattgaac tggccggttt ccacctgcga gttggcgagc
     8161 acgagcagct tggagacttg ctccactttc gagatgtgcg cgggtgtcag cgcgggcgga
     8221 tgacggtcag ggtcgttcat cagctcccgc gctttgcgga tgcgtgcgtc cccgtggacg
     8281 gcagcggcgc tgtcgagttt gtccagcagg gtgcgagcgg ccggataatc accagctttg
     8341 accagatctc ccgcccgcct gcatccggcg gtcacatagc catccaaagc cagcgcgcgg
     8401 aattccttcg ccatcggcac gtccggcagc gtctgccacg cctcttcgaa ggccgccaga
     8461 gactccaccg tttttccctc gtccaacaag cgcttgccct caaggagccg cgccgcctgc
     8521 tgctgaacca gttcggcccg gcgtgccgtc tccttgtcag caagggaggc gagtcctgat
     8581 gtctgtgccg cgcacggctc ctgcgtggcg cacagggcgc tgagcatgag catgaacacc
     8641 gccttgcgtg gcggcgtgga agtcaagcgg ggtggcgttt ccatcattcg ggatcaacaa
     8701 ttgctggcaa aatgcgacga ttatcaagtt ttcaggcgat tcccaagaac tttctcccgg
     8761 ttctgcaaag aattctcgcc tgcagatggg gccggctgcc gcccgccgcc tccggcttga
     8821 catgccgggg cggaaggcgt tcgctgctca caccatgaaa aaactcatct ccctgctttc
     8881 cgtcctggcc ttgtccagcc tcctccccct tgccgccgcc gacaaggcta aggacgttcc
     8941 ggtcagcgag gcggagaaac tggtcaagga gggcagggtg ctcgtgcttg atgtgcgcac
     9001 gcctgacgaa ttcaaggaag gtcacatcga gggcgcggtg aacattgact tcaacggcaa
     9061 ggactttacc gccaaggtga aggcgctgga cacagcgaag cccgtcctcg tccactgcca
     9121 gggcggcggg cgcagcgggc gctcgctgcc ggttttggaa cagctcggtt ttcccgccat
     9181 ctaccacctg aacgagggat tcggcgagtg ggaggccgct ggcaagccag tgaaaaagtg
     9241 aggcttgcgg cagggcggga tcgttgttta ggccgctccc gcaggcctag aaagagcagg
     9301 catcaccgag cttgtgaaac ttttcacaaa ttcgggttgc cgcacctgcc acggctccga
     9361 taatcgccca aacccctcct tttcccacat gcccgccccc gccgccgccg acacgcccgc
     9421 cgcgccaccg cctgttgacc tggtgaatgt gcagattgac ggagtctggc acaagttccc
     9481 caagggcacg cgcatgatcg aggcgtgcaa gcaggcgaaa aaggacgtgc cgcattactg
     9541 ctaccatccc aagctcacca gtcccggcaa ctgccgcatg tgcctggtgg agatgggcat
     9601 gccaccgcgt ccccaacccg gccaggagcc ggaaaaagac gcgaacggaa tgcccaaaat
     9661 ctcctggatg ccgcgcgccg tcatcgcctg cgcgaacacc gtggccgagg gcatgggcat
     9721 ccgcaccgac tccctgctca cccaggagtg ccgcaagggc gtgatggagt tcctcctgat
     9781 caaccacccg ctcgactgcc ccatctgcga ccaggccggc gagtgccgcc tccaggagtt
     9841 cagcgtcgag catggcaaag gcgagagccg cttccgcgag gacaaggtca agaagccgaa
     9901 gaatgtggac atcggcccgc gcgtgcgcct cgacgacgag cgctgcatca tgtgcagccg
     9961 ctgcatccgc ttcaccgccg agatcgccga cgaccccgtg ctcggcttca ccgagcgcgg
    10021 cagccacacc acgctcgccg tgcatcccgg ccgccgcctg gaaaacaact actccctcaa
    10081 cacggtggac atctgccccg tcggcgcgct gaccagcacc gacttccgct tccagcagcg
    10141 cgtgtggttc ctcagcgaga cggaaagcgt ctgcaccaaa tgcgggagag gttgcaacat
    10201 ggagatagga gcgcgcggcg acacccttta ccgccagaca ccgcgtgaga acaatgacgt
    10261 gaactccacc tggatgtgcg accagggccg tctggacttc cacttcgtga acagcgagtt
    10321 ccgcctcacc gagcctctca tcaagaccgg cggcaaacac gagtccgcct cgtggaaaaa
    10381 agccatgcag accatcgcat ccgccgcccc cggagccaaa ggcggcgagg tcgtcatcat
    10441 cgccagcgcc cgcatgacca atgaggaact ttaccttgtc aggcagctcg ccaggacact
    10501 cggcaccgaa aacgtggatg tcgtcccccg caccggtggc gcggacgcat atctgcgcgc
    10561 ggacgacttg aaccccaatt ccaacggcgt ccgccagatc cttggcgtcg aacccggctc
    10621 caggctcgac agcctgcgcg gccttctcgc ctccggcaaa gtccgcctcc tgctcgctct
    10681 gggtgaggac ttgctcaagg ccggtttcac cagggaggaa atcgaaaaag caggcttctt
    10741 cgcctcgctc cacatcctgg cccatgcctc cgccgaactg gctcaagtgg tcctgcccgg
    10801 cagctcctac gccgagaaaa gcggcagcat gatcaacgtc accggccgcc tccagcgcct
    10861 caacaaagcg gtcaacagcc ccggcaacgc gggcgacacc tgggaaatcc tgcgcgacct
    10921 caacgtcgcc tgcggtggtt cgaacggact gcacagcatc gaggatgtgt tccaccagct
    10981 cgctgcggaa gtgccctgct tcgccgggct cagtttcgcc aaaatcggct cgctcggcac
    11041 gccggtgatc gagactggcg agactgtccc cattttggag cgcgagaaag agcgcaaggc
    11101 caggggactg atcgttggtt gatccctccc ccgtcctgga gcgccaggcc atccctcact
    11161 ccagcggctt gcccttggtc tcaggcgcca gccagacgat ggccacgccg acgaagtaaa
    11221 ccatgcacaa ggcgatgtag gcattggccg aggacgcggc gccattgccg ccgaagaggc
    11281 ccacgagatt cgcaagctgc agcgcgccca cggcggcgat gatgcggccc acattgaagc
    11341 agaagccctg gccggtggcg cgcacgcggg tggggaacaa ctccggcagg taaagcggga
    11401 agaagccgta gaacgaggcg ctcacaccgc cgatgatgaa ggcggaggcg aagaaccagt
    11461 ttgagatttc cgtgttggtt ttataaaaca gcaccgcgct gccgaaagcg gccaggcagg
    11521 tcagcgagta ggtcagcctg cgcccgaaaa tatccgccac caccggcatg aagaaagtgg
    11581 ccaggatggc gccgaaggag gtggcgagct gcgtgtactc gcgcgggtgc agccagccgt
    11641 tcgcggccag ctccgtggac cattttggcg cctgctgcgc cgcgccccag gtgcccagca
    11701 gcgccacgcc tgccaggctc gcgccgatga gcatgtggcg gatcaccacc ttgcggtcca
    11761 ccgtgcccag cgccccggcc agggccgcgc gcgccagata gcggcgcacc gggaagaggt
    11821 agccccacag caccgccgcc aggccgaaga gcgtcaccac gccggcctgc gccgccgagg
    11881 gcagccccat gggcgaccac gcccagatga tgaccatgcc cgccatgccg ccggtgagca
    11941 cgccatagag gtcggtgttg ctccagtgcg aggtgttccc agcctccttt tcctcgtgcc
    12001 acttctccga ctcagggacg aagaggcgga tgaaaaagat gagcagcgcg gggaaggcgc
    12061 cgctgatcat caggaagcgc caggcgctgt tgtggaaaag gctgtccgcc cattccttcg
    12121 acaggccaag gcccatactc atgccatgcc aggactccac ataactgttc agccccaggc
    12181 tcaggaaggc caccagcagg tagccgatgt tcgccgccgc gccgatggcc ccggccaccc
    12241 acacgcggtt gcccttcgtc cacagctcgt tcaccagcgc cacgcccagc gcccactccc
    12301 cgcccatgcc cagggaggcg atgaagcgca ggatggcgat gtgccaggcc tccgtggcaa
    12361 agccgcacag gcccgtgaag atggcgtaag tgaaaatgga gaaactcatc gcccgcacgc
    12421 gcccgatgcg gtcccccagc cagccaaaga gcacgccgcc cgtggccgcg ccgacgagga
    12481 aggtggcgat gatcaccgag aaccagttcg tgatgaccac cggctcctgc cctggcagca
    12541 gatccttgag cgcgggcacg ccgatcaacg ggaaaagccc catctcaaac ccgtcgaaca
    12601 tccaccccag aaaggcggcg atgagcgcca tgttgcggcc ggtggcggtg gtcttgggtc
    12661 tggacatgat gatttttggg gagcgtgaat tacgggcggg aaacggcatt cttgggacaa
    12721 ttcaccgcgc gcgacaagcc tgaatctccc cacctcattc caccgcatga aaaaggcggc
    12781 ctcctcgcgg aggccgccct ttggaaaagc acacggctta ttccctcacc ttggcgctct
    12841 tgatgagcac cggctcggtg ggcacgttgg cgtggccgct ttttgtgcca gtgggcacct
    12901 gggcgatctg gtccaccacc tccattccag cgctcacctt gccaaacacg cagtagcccc
    12961 agccatcgcc gccgggatag tccaggaagc cgttgtcctt caggttgatg aaaaactgcg
    13021 aggacgcgct gtgcgggttc ggcgtgcgcg ccatggcgat ggagccgcgc gcgtttttca
    13081 gcccgttctt cgcctcattt tggatcgggg cgtccgtggc cttctgctcc atgtccggcg
    13141 tgaagccgcc gccctggatc atgaaatccg ggatcacgcg gtggaagacg acaccgtcgt
    13201 aatgcccttt tttcacataa ctgacaaagt tggcgacggt gcccggggcc ttggcggcgt
    13261 cgagttcgag ttcgatggtt cccttgctgg tctccatcac gatggtcacg ggtttggctt
    13321 cgctcacggc gggtgtttcc tgggctgggg ttgtaccggt caagccggcc agtgcggcgg
    13381 caaaggcggc aagtaccgga aggcggggtt tcatgtctgg cttggttttc ggcgctcaaa
    13441 aagcatgcgc ccgcgccttt gccaagcgaa aagcacgccc cgcctcacct ccgcagcgcc
    13501 gtgatcagct cgtcgagctt gtcggcgagc tgctgcacct ccgactgcgt cggcgggtcg
    13561 ctgatgctca ggcccagcgt ggcgacgccg aagctgttgg cgctgcttgt ccgccggccc
    13621 gccggtgatg ggcacgggga cgaacttcat gccgggggtg ttgacgaagg gggcgtcttt
    13681 ggtcgctgcc gcgatggaag actcggagac ggggagacag aactggtgcc aacaggcgcg
    13741 gcggacagca cgcagcggaa gccgctgaag acacgcatgt tgggggcgtt gtggttgcgt
    13801 ttggaggaga gcagggcggt gcggtcgctc atgtcccacg aggccccgcg actcacgcgg
    13861 gacgttttcc tctggtcgaa ccagtcttcg caccactccc acacgttgcc tccgaggtcg
    13921 tggaggccgg agcggtccgg cgcctgcgct cccgcggggc ccaccgtggc ctggccatcc
    13981 tgatagcccg gcagttcccc gctgatgaag gcgtacttcc ctgccgccag cagcgggcgc
    14041 agttcctcgc tccagtaatt gccggagttg gcgggcggcg gccactggct gccccaggga
    14101 tagacatcgg cgagcttgcc atccttctcg tgcggcaact gcgccgcatc ttcgcgcccg
    14161 ccgatgtccg ctgcgcagct ccattcgtgg tcggtgggca gtctgtaggt ctcgcccgcg
    14221 gcaagcctgc ccgcggcgcg ttcgcgctgc gtgagccagt cgcaaaacgc cttcgcgtcc
    14281 tcccaactga cgccagcgac ggcgtggccg ggcctttgct ccacatccgg gcgcttggcc
    14341 cacgaatgtc cgctctcctt tacgaacgcc tcgaagtcct gcacccgcgt ctcccacacg
    14401 ctgaaaagca cccgttgttt gtccgtcggt ccgccggtga cgggcacggg gacgaacttc
    14461 atgccgaggg tgttcacgaa gggggagtcc ttcgttgccg cgctgggcga ggcgggtggt
    14521 ttcacatcac tcctgcgcac tgggccgctc gcggcggctg ggggaccagc cgtcctgcca
    14581 tcgcctgagt cgcgtagcgc gaagaacgcg ccgactgcga ggaccgcacc gacaacaagg
    14641 ccgaagatca gcggggcgcg ggatttcgca gccgcgctcg caagagcgcg gggcttggtg
    14701 gctgcttgtg gcttttcggc cgcgtccttg cggacgcggc tgcccgccag cggaatgctg
    14761 gtgacatccg tttttacctc gcgggcctgc tggtagcggt cgccgggatc gttctgcatg
    14821 gcgcgggaga cgatgtcgtc ccactgcggg gccaccggcg cgcgctcgga gggtgccttc
    14881 cacgcgccgc gcgggagctg gccggtgatc atctggtaga tcatgacgcc gagcgcatag
    14941 atgtccgcgc ggtggtcgat ggtggcgttg ggatcgaact gctccggcgc ggcgtagtcg
    15001 ggcgtgccca ttccggtgcc ggtctgagtg cggaagctgc tctcggcatc gaaatgcttg
    15061 gcgaggccga aatccgctat cttcacgcgc ccctcgcggg tgagcatgat gttgctgggc
    15121 ttgatgtcgc ggtggacgat gccgtggtcg tgcgcgaact gcagcgcgtc acagatctgc
    15181 ggcagcagag tgagcgccat ctcctgcgtc atccggccgg tgcggatcac gtccatgagg
    15241 tccgcgccgt ccaccagctc catgacgatg tagaggtaat ccggcccggc ctcgccgtag
    15301 tcatgcacac cgacgatgtt cgggtggctg agcttcgcca tggcctgcgc ctcgcgctcg
    15361 aagcgcatcg cgaagtcgta atcccgcccg gcctcgcggt gcatgatctt gatggcgaca
    15421 gggcgcttga gcttcacatg gatgccttga tacaccgcgc ccatgccgcc ctggccgagg
    15481 aagccctcga catgatacat gccgtgcggc agcagggcgg tcagctcctc cggcagcggc
    15541 agatccggcg cctgatgcgc cggctgggcg ggcgcggacc gcgccatggg gatggtggcg
    15601 gcgtccccct ccgcggcggg ccgcatggcc tccgccatga ggcaggcgtg gcagagtcct
    15661 ttggcatccg ggtccgccag gggtgagccg cacttcgggc aggtgatcgg ggagtctggg
    15721 gaaacgggat cgctcatgcc ggtgtgctga atcgtgccga gggaaaagtt gcaggcaatc
    15781 tcatgcgcgg cgcagcgcgg cgaagagggc ggccatctcc tcctcgatgg agtcccccgc
    15841 attcagcgtg ccggaaacct cctgccgtac cagctcgcgg tagcggcgct tcaggcggtg
    15901 gatctcgctc ttgagcgtgt tgaggttcat gccgagttgc gcggcgagcg cgaactggtc
    15961 gccgtgctcg gactcgccgg cgagccacgg tttcgcggca tcgtggagcg ccgccttgcc
    16021 tttcgcggtg cattccgcct gcaaggcatc aagcgcatga tccagcaagg tgcgcgccca
    16081 caggcggtcg aagatgcggt cggcggcggg ctgcgtcgcg tccgccacct cgcgcgcttc
    16141 ctcgtcatcg agcgacgcgg cctccacgcc gccgcctcgc ttcatgcggc tggcatggag
    16201 gcgctggcgg aggaggaaat gcttcaccgc gcccagcagg taggaacgga agcgcccgcg
    16261 ctcccgctcc gcatggtcaa tcgtgccgcc gccgagcatc tcggcaaaga aggcgtggct
    16321 catgtcccgc gccgcgtcgg catccctcag ctcgcagcgg aggaaggcga cgaccggctc
    16381 gtagtaggcg tcacaaaggt ccgccagtgc cttgcggccg tcctcggtgt ccgccttggc
    16441 cgcgcagacg cgcgtccagc gcgtggtgtg aaaagaagcg tcgtttgagg cgggtctcgg
    16501 ctcaggggtc atggtccggc aggcacgctg ccacaagtcc ctgacattgt ggaggggaaa
    16561 gatggcggtt tcgccgggtg tttgtcagcc ggcggctgcg cggctcactg cgccgccggc
    16621 gccagcacgc agcggaagcc atagttgggc tggcgagtcg ccggcggggg cgtgatgcgc
    16681 catgaggaca gcatgacgcg ttctttcccg tcaacacagg aagcgccgcg caccaggcag
    16741 ccctcctgcg agtccgcgca ccattcccaa gcattgccgc ccaggtcgta aaagccgtgc
    16801 ggattcaccg cgaaacgccc cacgggcgaa ctgtgcgcaa agccgtcgtt gtagccctcg
    16861 agcatgatcc tgccgccctt tccagactgc gggtcgtccc ggaactcctc cccgctgtaa
    16921 ttgccggcgt ccggtggtgg cggccactgg gcaccccatg gatagagggt tgaaattctc
    16981 ccgctctttt ccttcggcgg ctttgcggcg tcctcccggt cgccgatccc cgccgcgcag
    17041 ctccactcgt gatcgcccgg caggcgatag gacttgtccg tgccgagctg gccggccttc
    17101 cgctcgcgct ccgtgagcca cacgcagaag gcggcggcgt cctcctcatt caggttcact
    17161 gcgggatgtg tcgccccctg ctcgaaatcc ggcttctccc atttgcgttt cgtctccttg
    17221 gcaaagacct catagtcctg catccgcgtc tcccacacgc tgaagagcac ccgctttccg
    17281 tccgttggtc cgccggtgat cggcacggga acgaacttca tgccgagggt gttcacgaag
    17341 ggagtgtctt tcgtggcgat cgcgacggaa gaagaggaga cttggagact cggagatggg
    17401 gagacggcgg tggttttgtc gaggtcgagg aattcgacgg actgatacca gccgtcattg
    17461 gcgttgatgc cccattcgcc cgctcccgtc acgcgggcat catgggcgtc gatcacggtt
    17521 tcaccgttca gctttccgat gaggtgatcc ccctgcgcgc gcaattccag ttccactgtc
    17581 tcgccgggct gtggaggttt cgggatcgca tgcttggcga tggtttccgc tttcccgccc
    17641 gacatcccct gcaggtaaac gaaggtaggc gggttgctga gctcgagctt gtagaagctg
    17701 tggccgaccg cgagccgagt cacgacactc gggttgcgcg tgtcctgccg gaactgaaac
    17761 cgcgcccgaa tggcgccatc gggagatgtc tgcggcttcg tccactggtc tgctttcaga
    17821 tgcttcagcc cgtctttgaa ctcgccgtgc gagccggttt gcttccaatc agaactggtg
    17881 aagagcggct tccacccgga ctcggggaga gcgggggttg cgatgggcga ggacgcggct
    17941 tccggtgggg cggatttcac aacggtggac gcgatctcac cccattctat cttttcgatg
    18001 accgtttcca gctcagcccg gaatcgtatg taaccactcg tgagccgtgc gtcgttgacc
    18061 ttgcacatca aagcgccatc gagccatatc gccagttcgt cgcctagtgc agtgacaatg
    18121 ctctcgtgaa agctcgtggt tttaaagcct ttgaccggca gtgactccgc caatctcaaa
    18181 tttggttccc tctctccatt ctccagcgtc gtcttgtcaa gtttgccaag ttcgatccat
    18241 gcttgcccaa tgccattgat ggcagcgcca taccattggg tggattcggt tgaggtcgta
    18301 tgacggaact taaaactgaa tgggccttgg tggcggatgc ggatggccaa attggcggct
    18361 ttatcccctt gaggaacatg gatgttcggg agcaatcgcg taggccgcca gccttttggt
    18421 agggcttctg tggaatcagc gcccgcgctg tccctgatgg ccgtggtcac atcaatccac
    18481 atcttgggtg cagggatcgt cgggctggca gctgcggcgg gagcattctt gaccggcgaa
    18541 cttttatcag tgtttggtag agtggttgcc tctccctgtg ctgctggagg atgcgtttct
    18601 gtggcgacat tcaaaggcgg ctggaaacct ccactctttg gggcggcgtc aggcagcgca
    18661 aagaacgcac cgacggcaag ggtgatcacg gtgccgatga cgccgaagag cagcggggcg
    18721 cgtgatttgg attgtagggc aggcgctccg cctgccgggg tgatagccgg gccgggcaag
    18781 cggggcgctt gccctacagc gactgatggc gtgatccgac caacatcggt cttcacctcg
    18841 ctggcctgct ggtagcggtc tgccggatcg ctctgcatgg cgtgctccac gatgtcgtcc
    18901 cactggggag cgacctccgc tcgctgcgag ggcggcttcc acacgccgcg cggaagctgg
    18961 ccggtgatca tctggtagat catgacgccg agcgcgtaga tgtcggcgcg gtggtcaatg
    19021 tggccgtcgg gatcgaactg ctcgggggcg gcgtagtcgg gcgtgcccat gccggtgccg
    19081 gtctgtgtgc ggaagctgct ttcggcatcg aaggtcttcg ccaggccaaa gtcggccatc
    19141 ttcacgcgcc cgtcgcgggt gagcatgatg ttgctgggct tgatgtcgcg atgcacgatg
    19201 ccgtggtcgt gcgcgaactg cagcgcgtca cagatctgcg gcagcagagt gagcgccatc
    19261 tcctgcgtca tccggccggt gcggatcacg tccatcatgt ccgcgccatc caccagctcc
    19321 attacgatga agaggtagtc agggccggcc tcgccgtagt caatgacgct gacgatgttc
    19381 gggtggttga gcttggccat ggcctgggcc tcgcgctcga agcgggcggc aaaatcaaaa
    19441 tccttcccga gggcgcggtg catgatcttg atggccacgg ggcgcttcag gcgcacctgc
    19501 atgcctttat agacggcacc catgccgccc tggccgagga agctctcgac gtggtagttc
    19561 ccgccgggca gcagggcggt cagctcctcg ggcaggggca gatcgggaac ttgattcact
    19621 ggctgggagg gcgcaggccg tgccaccggc agtgtggggg cttcgtcatc cggcgcgggt
    19681 agcatgacct cggccatgag gcagcggggg cactggcttt ccggcacgcc gggggggagc
    19741 acggtgccgc agtgggagca ggcgggatcg ggagatgaag cgctcatggc tgtaaactga
    19801 cgcgtccgct ctggaaaccg aacaaaaaaa tgtcatccgc tcagggcggc gaagagcgcc
    19861 gccatctcgt cctgcacggt ggcggggtca tccagcgtgc cggccacctc ctcctgcaca
    19921 gactggcgca ggcgcttttt taaccgggtg acggccatgc ggaaggcgtc gaaagtcatg
    19981 ccgcattcgg cggcgagcgc ggtctggtca ccgtgcgccg cctgtccgct gagcagcccg
    20041 cccactttct caaagaactc cccgcgcccc tccgcctcgc accaggcgcg cagcgcggcc
    20101 atggagcggg tgatgacggt gatggcccat tgccggtcaa actccgtgtc gggagagggc
    20161 tgccgcgcgt ccgccacccc ggcggcttcc tcatcatcca gcccgatcac gctggcgcct
    20221 ccaccgcgct tcaagcggcg cgcggcctca cgttgatgcg agacgaaatg cttcaccgcg
    20281 ccgagcaggt aggagcggaa gcggccgcgg cccgcgtccg ccgtgccgag gcggcctccg
    20341 gcgaggattt ccgcaaagaa cgcatgactc aggtcgcgcg cctcgtccgc gtccctcagc
    20401 acgcagcgca agtaggcgac gacaggctca tagtaggcct cgcacaaatc cgcgagcgca
    20461 agacggccct cctcggaatc gcccttcgcg agacgcacac gcgtccagcg cgtggtgagg
    20521 aacggcgcgg cggtagagtc ggctttcagc tcagggttca tggtccggcg gccagactgc
    20581 cataagtccc tgaggttttg gagggaaaag gtgacggcgc ggtatatttt gaaaaatgcg
    20641 cacacggatg ccgattggag ctgtcctgcg tcagcatggg agcgcgaatg gcttgactca
    20701 tgccggggag gccagagggc tgcggcatgg cggctgcaat ccgtctcatt ggccgggaaa
    20761 attcaaggcg tcatcgcgct ctcaccggcg gcagggatcc cgccatcttt gcgcctgtgc
    20821 gttgaagttc ggcggcggcg gcggcgcgga gtttgttcag caactccgcg ttcccaggtt
    20881 gtccggcgat gttggtgagt tcgtcaggat cggacagcag gtcgtagagt tcctcgggtt
    20941 cgccagcttc gagagtacgg atgtatttgt gatgaccgct ggcgagcatc acataccacg
    21001 ggatgccggt tttgtggtga ttgccgggcg gagcgggcac cacgtcgcaa tccgcgccga
    21061 atttgtcggc ggtgaagggc atgatcgtcg tgtgcggcca ggcgccgtcc ggattcttca
    21121 gcagcggcgt gaggtcgtgt ccgtgcattt cccacggcag atccagaccg gcgaagctga
    21181 agaaggtggg cggcagatcc gcgccgccga cgggcgtgcg gcagaccttg ccttcgggca
    21241 gtgtgccggg catggaaatg atgagcggcg agcggatgtt ggcatcgtag ggcgcgagct
    21301 tggcgcggaa accgtggtgg ccccatgcgt aaccttgatc agcggtgagg acgacgagcg
    21361 tgttgcgcaa ctgcccgctc tcctccagcg cggcgagcac ctggccgatg ccttcatcga
    21421 gcgaacgcac ggcttgatga tactggcgcg accacttcga gagcgagcgg ccgcgatcgc
    21481 gctcgaaatc atcgcccacc tcgctgccga tggccttgtc actgagcacg ggttcgccgt
    21541 cggggccttt ttcccacgcg gcgatctttt gcatgtaacc gggcttgcct gggcgcggtg
    21601 gatagatgtc cgcaggcgtc gccacggtga caccgggata atcgtcgaga tgacgttccg
    21661 caggcgtgta gggcgcgtgg acaccgccgt agcagagcca gagataccac ggtttgtcgg
    21721 cgtcgcggtt cgcgcccttg atgtagtcca cggcccagcg tgtgtagttg tcggtggagt
    21781 agcccttcac cacatgcggc tcgccgccat tgatggagag gagttgatca tggtagtagt
    21841 tcggcgcgtt gtcgggatga cgcgggcggt tccagacgat ctgatgatcc cagtcgcggc
    21901 cgtagccggc gtcgttgccg gcatgccatt tgccgatctg cgcggtgtgg tagccctgct
    21961 ggcggaaaac ggcgggccag aagcggcact gccgcggatc atacgtcgca ccgggatacg
    22021 gcggcgtcat gcgcatcgtg cgcacgccgt attgatgcag gccggtgagc atggtggtgc
    22081 gggaggacat gcaccaggtg ccgatgtaag catgtgtgaa acgcacgcca cgagcagcga
    22141 ggcggtccat gttcggcgtc tttgcccaag actccgcgcc ttcgtagcag gagagcgtgc
    22201 ggtaggagtg atcgtccgtg tagatgaaaa gaatgttcgg cttcgccgcc gcgtcaaagg
    22261 cgagcagcag gcatggggcg aggatcaaaa gaacggagcg gttcgagaag tctggcatca
    22321 cgacggaacg acacaaggcc agcccgattt cagcgaactc acatcccgct accgctcagc
    22381 gttcaacgcc cgccggatgg cgcgggaatt cgcgatcatt gcacgggctg cgctcgtaga
    22441 actcttgcgg gcgggcttcg ggggagttcg ataaggcgga gacggcggcg aggttggaaa
    22501 gcatgttcga tggggaagtg tgagtgctcc gtcttgccga gtctgtagag tcaatggtcg
    22561 cttcacccga caaggtccgc cggtaaaaag cccggttgtc ctcgccgagc gtgtcgagga
    22621 ccatgttggg gtcgtgaggg atgccgggga actggcggta agtatgcggg atcttcaaag
    22681 cgtcgagatg ctgcttgaac tcgcgattga agtgcagagt ctcatcgcgg tcgccgatga
    22741 tctggcggat ggggaggcct aactatcaac caaaccgaca ttggaatttc tcgtgcttgt
    22801 ggactacccg ccatttgtcc tggcgggttc tccctcagtg agattttgcc ggtttggcag
    22861 gctatgcctc gcgcagaagg atggcatcca cgttgactcc gtcgtaaagc gcgtcgctga
    22921 gaatgacgag ggggtcgccg ggcttgatga ggtcctcccg gcgcagcagg ttgatggcgt
    22981 cgcggatggc ggcctcgggc tcgccttcgg caaagggcag gacgaagggc tgcacaccgc
    23041 gtgcggtgac cagggtgcgg ctcacatgcc ggtgcggcgt gaaggcgaag atgggcgcga
    23101 actccggcct ctgatgcgcg agcaggtgcg ccatgacgcc gcgcagagtg aagaccagca
    23161 gtttggatcc agggatgctg ttggccagca ggatggcggc cttcacggtc ttgtgcttgt
    23221 tcgtcttcaa tggcgcgtcc gaggcgaaac ggcccgaggg atatttcttt tcgatgcggc
    23281 ggatgacggt gtcgagcacc tcgacgcatt tctccgggta gcgcccgaca ctggtctcgc
    23341 cgctgagcat gacgcagtcc acctgctcga tgacggcgtt gaaaatgtcc gtgacctccg
    23401 cgcgggtggg cacggggttt tcgatcatgc tctccagcat ctgggtggcg acgatgacct
    23461 tgcgtccgtg gtaggagcag cgttcgacga tctggcgctg gatgatgggc agctcctcaa
    23521 tggggcattc gctgcccagg tcgccgcgcg cgaccatgat gccggcgctg gcctcgacga
    23581 gcgtgtctat gttttgcagc gcctgctggt tctcgatctt ggcgatgaca cgagccttgc
    23641 tgttcttctg ctccagcaga agctgcaagt gctggatgtg gcccggctcg cgggcaaagc
    23701 tgagggcgaa aaagtccacg ccgagctcga cgccgagatc aaggtcggcg tagtccttct
    23761 gcgtgagcgg cggcagccgc acgggcacgc caggcaggtt gatgtggcgg cgggacttca
    23821 tctccccctt ggtgagcacg gtgccgagaa cgcggctgga gcagacttcg gcggctttga
    23881 tctggatcat gccgccgtcc accatgatga tgtcacccgg gcggaggtct ttgccgagtt
    23941 cggggtagtt gaccgtgaca gaaagcggga cggtcggagc ggcttcgggc ttgcagcgca
    24001 gctcgacctg atcgcccggc tgaagctgcc agggctggtc gagatcgccg gtgcggatgc
    24061 tcggtccggt gaggtccatg agcacggaga tttccttgga acccctggtg gcggcctcgc
    24121 gcacacggcc atagacggtc cgcacccagt cgtgggaggc gtggctcatg ttcagccgga
    24181 aaatgtcggc tcccttggag atcagctcgc tgattcgttc ggcggattcg gtggcgggtc
    24241 cgagggtgca gaggattttg gttttgcgca tgtgcggcat ggtggggacg gcgcgggggg
    24301 attcaagcgc cattcccgtc aggaagcacg ggacaggcca cgtcacgcag tctcgaaaag
    24361 aaaagcgcgg acacctttcg atgcccgcgc ctcgtgaatt ccatcggacc ggccttatgc
    24421 cgccgcttcg gcggcggcgg gctcgacggc ctcctccttg acggtggcag gcacataagc
    24481 gtccacccac tcgatgtagg ccatcggggc ggaatcgctg cggcgctggc cgagcttggt
    24541 gatgcgggtg taaccgccgg cgcggtcttt ggaggcgacg gcgatttctt cgaagagttt
    24601 cttcaccatg gccttgctgt gcagagtgcc gacggcgagg cggcgcgcgt ggaggtcgcc
    24661 acgcttgccg agggtgacga gcttctccgc gtatgggcgc agcgccttgg ccttggccag
    24721 ggtggtgcgg atgcggcggt gttcgatgag cgcgcaggtg aggttggcca gcagggcttt
    24781 ccggtgggag gcgtcacgct ggagcttggg gacttttctt ccgtgtttca tgatgtttgg
    24841 ctttcaaagg gcgggtttgc cgggtttatt cgtcgtcttc cacgtcgggc aggttgctgt
    24901 ccacaaggtt ggtgaagtcc accacgtctg cgtcctcgtc ctcgtcggtg atcatgctgc
    24961 tgcgtgcgag cagggacacg ccgccggcgg agggttcgag cagggatgcg tcgaatttca
    25021 tgccgaggga gagaccgagc tcggagagct tttccttgat ctcggtgagg gacttcttgc
    25081 cgaagttgcg gtagcgcagc atctcggcct cgctcttcat ggcgagctgg ccgacggagg
    25141 tgatgttggc gttgttgagg cagttggcgg cgcggacgga gagctcgatc tcgttgacgc
    25201 tcatgttgag cagcttgcgc agctcgctgt tctcctcggt ctgggcttcg ggggcggcct
    25261 cgaactcgat ggccttgtcg tcatagttga cgaagacatc aaggtggtgg cgcaggatgg
    25321 cggcggactg gagaagggcg tcgctggggg aaatgcggcc gtcggtccag atgtcgagca
    25381 ccagcttgtc atagtcggtg ttctggccga cgcgggtgtt ttccacggcg tatttcacgc
    25441 gggtcaccgg gctgtagatg gagtcaatcg ggatgacgcc gatgggggtg tccgggcgct
    25501 tgttctcctc ccaggaggag aagccgcgac cgacgcgcac ctcgaactcg cattcgaatt
    25561 tggttttgcg gtcgagcgtg cagatgacct ggtccttgtt gatgatctcg tagtgattgt
    25621 cgtccttgat gtcgccggcg gtgacgacac cttccttgtc ggctgtgacg gaaagaaggc
    25681 ggggttcctt ggtctcgctg tggtgcttga agcggacttt tttcagattc agaacgatct
    25741 gcaccacgtc ttcgacgact ccggggatgg tgctgaactc gtgctcgacg ccgcggattt
    25801 tcacggaggt gatggccgcg ccctccaggg aggagagcag cacgcggcgc agggagttgc
    25861 cgatggtgtg tccgtagcct ttgtcaaagg gctcggcgac aaactgggcg taggtgtcgg
    25921 tggcggtggc ctcgttgcgc acgaggcggt tgggcatttc gaatctggct agacggacgg
    25981 acatgtgggt ggatggggtt gccgggtttc ctccagggcg agctccggtt cattcgcaag
    26041 gaatggccgg acctggagac cgacggcggg agtgtgacgg ctcgcaaggg gccgtgatta
    26101 tgagcgttga gaagctgttt gcaaccgcat tttccgctca atttcggggg tgttttccgg
    26161 cgttgcccgc cgcttggagc gctccacttt cccgcccgca tgaagggccg ctttgccatt
    26221 tgcctctgcc tcgccgccac tgccgcctgc gcgggtgcgg agccgcgcgc tccctggtcc
    26281 ggttcccgcg tcaagggatc cccggagccg ccgccgcctt atgtggccgc gccggttttc
    26341 gagaccctgg agctttccga ggtgctggag atcgttccgc atgacgggag cgtggtcgtt
    26401 ttggaaaatc gcggcagggt ctggtgcttc gaaaaccatc cgcgcgatcc cgggccgcca
    26461 tttctgatgg ccgatctcaa agtgaagcac ccggcgctgg accatctcta cggcatcgcc
    26521 ttccatccgc gctggcggga gaaccggcag gtctttgtca cctacacggt cggggcgaag
    26581 atcgcggacg gcacccggct ctcgcgtttt ctgctggatg aaaatgaccc gccgcgcctc
    26641 gtcctcgaaa gcgagcaggt gctgctgacc tggctctccg gcgggcataa tggcgcgcat
    26701 cttcagttcg ggcctgacgg catgctttac atctccaccg gcgatgccga agcgcccgct
    26761 ccgccggacc tgctgaacac cggccaggac aacagcgacc tgctttccgc catcctgcgc
    26821 attgacgtgg atcccaccgg gccagccctg ccctaccgca tcccgccgga caatcctttt
    26881 gtcggtaaaa gcggcgtccg cccggagata tgggcattcg gttttcgcaa tccgtggaag
    26941 atgagcttcg actcgcgcgg gcggctgtgg tgcggcgatg tcggctggga gctgtgggag
    27001 atgatccatc tcgtcacacg cggcagcaac cacggctgga gcgctttgga agggccgcag
    27061 cccatcaaac tggaaacact cagcgctctg gcgcccgtga cagcgccggt ggccgcgcat
    27121 ccgcacaccg aggcggcctc catcacgggc ggttatgtgt atgagggcaa aaagctgccc
    27181 gccctgcgcg gcgcctgcat ttacggcgac tacgagacgg gcaaaatctg ggcgctctgg
    27241 catgatggcg gcaaagtcac gcggcacgag gagatcgccg acacgccgca ccgcatcgtc
    27301 agcttcggcc tgctgccgga cggggagctg gtttacgcgc actgggacaa aacagcggcg
    27361 tttttcgaac tggtccccaa ccctgaaacc gggcgcgcgg aagaatttcc acgccgtctc
    27421 agcggcactg gcatcttcca aaacacggcc gcgcagacac tcgcgcctgg ggtttatgaa
    27481 ttcgatatcg ccgagcctat gtggcaggac ggacttgagg cgcggcgctt catcgctctg
    27541 ccgggcgcgg gcgcggtggc ctccacgatt cagcgccgtg ctgacggcac cgttcaacgc
    27601 gccggcacgc agtggcccgc tgacagcgtg ctggtcaaaa ccctcgtcaa cgagtcccgc
    27661 cccgtcgaaa cccagctcct gcattatgat ggagctcact ggcagggcta cagctacgcc
    27721 tggaatgaag cgggcacgga cgcggaactc gtcccggcgg agggcgcgca aaagcagctc
    27781 cacggacgcg cccacgccta ccccgcgcgc gccgagtgca tgcgctgcca cacgccgtgg
    27841 accgggcacg ccctcgcctt ctgcccgcaa cagctcgcgc ctggcgctgg agctgatgtg
    27901 ctgcgcgcgg atgacgccct gcggctcggc ctggtgaacg cggagttttt tgagacctcc
    27961 agcgcgcgtc tggccggcac ggatgacgcg ccgctcgaag accgcgcgcg tgcctggctg
    28021 catgccaact gcgcgcactg ccaccgcctg cacggcggcg gctccgtggc cttcaaagcc
    28081 aatatcgaac tggaactcga cgccatggaa atgctcgatg gcgctccctt gcggggagac
    28141 ttcggcctgc ccggagcgcg catcgtggcg ccgggccgcc cctgggccag cacgctcatc
    28201 gcgcgcaccg cccagggcgg tcccggccac atgcccctca tcggcgcgcg cgaggtggac
    28261 acgcgcggac tggacttgct ctggcgctgg atcgagagcc tgcccgcgcc cgccgggagc
    28321 ggagaggggg acacagattt cgctgccgcg ctcaagcagg cgctcgctga cggcaggcgg
    28381 gactcctgga acaaactcgt caacgaccgg cctgaccgcg ctttgaacct ggcgcggctg
    28441 cttgatgtgg atggcaccct gcggctcgcc ttcatggagt ccacccacca tctggacgcg
    28501 catcccaatg ccttcatcgc cgccttgttc gagcgtttcc tgccgcctgg gaaaaggcgc
    28561 gaacggctcg gtgccgcgcc agttcccgca gccattctcg ctttgaaagg cgatgccgcg
    28621 cgcggagccg cgctgctttc accgttgggc gcgctggcct cctgcctggc ctgccaccgg
    28681 cttggcgacc gcggcatcag catcggccct gacctcacac atgccggcgc gcgcctcacg
    28741 cgggagcagt tgctcgaaag cctgcttcag ccctcgaaga gcatcgcgcc ggagtatcgc
    28801 gcgcacagcc tcgtcacctc cgatggcgag gtgcggacct gcttcgtttt gaatggcacg
    28861 gaccccgaag tcaccgtcaa gctgctcacc ggcgaaacgc tcacctacgc ccgcgagggc
    28921 atccagctca cgccgctgcc cgcctccctc atgacggagg ggctgctgca atccctctcc
    28981 gcgcaggacg ccgccgatct gctggagtac ctcgccagcc ggaagtgagc gcgtctttat
    29041 ttcagcccgg cttccgccag ccgctcctca agatcgctgg ccagcaaagc ctgggtcatc
    29101 gcctccatgc ggcctaccat gaacatgtcc agattgtaca aagtcatgtc aatgcggtgg
    29161 tcggtgacgc ggttttgcgg gtaattgtag gtgcggattt tttcttcgcg tccgcccgat
    29221 ccgatgaggc tttttcggtg tgctgaatat ttttccgcct cctcctgctg cttcttctcc
    29281 agcagacgcg agcgcaggat ggtgagggcc ttttccttgt tcttgatctg gctgcgtccg
    29341 tcctggcagc gcacgatcat gcccgtgggg aggtgcagca cctgcacggc ggagtccgtg
    29401 gtgttcacgc cctggccgcc cgcgcccgag gagcggcaaa cttccacgcg aatgtcctcg
    29461 gcgcgcaggt ccagatccac ctcctccgcc tctggaagca ccgccaccgt ggccgccgag
    29521 gtgtgaatgc ggccctgcgc ctcggtcgcg ggcacgcgct gaacgcggtg gacgccgctt
    29581 tcatacttga gcacgcggaa gacatcctct ccgctcactt tcattacgat ctccttaaag
    29641 ccgccgacgt cagaggggct ggactccagc gtctccacct tccagccgcg cgtctcggcg
    29701 aaacgcgagt acatccgcac caggtcggcg gcgaaaagcg atgcctcatc cccgccggtg
    29761 cccgcgcgga tttccaccag cgcgtcgcgc ccctccagcg ggtccggcgg caggatggcg
    29821 aactgcaccg ccttctccag ctccgcgatc cgtttctcca gcagcggcag ctcctgtcgc
    29881 gccatcgccg cgaactcgtc atcctcgccg cgcgccaggg tctggctttc ctcgaactcg
    29941 gtcctgagct tttgcagcgc gtcccagtcg ctcagcagcc cggtcaactg gcggtgctcg
    30001 cgcgtgagtt cgccggcttt tttggggtcg tcaaaaaaac cgggctggcc catcagctcg
    30061 ccgaggaggt cgagacgggc gcgtttgctg gtgatgatgg agctgtagtc catgggtcag
    30121 gcggggtcgg acaaagaaaa acggtgccgg ccgggaggcg cgggcaccgt tttcggaaat
    30181 caaactgtcg cggtctagga ggcggcggcc tcgctgactt tcggagcgga gcggcgggcg
    30241 cgggtgctgc catagcgctg ggcgaacttg tcaacgcgcc cggcggtgtc aatgaagcgc
    30301 tgctcgccag tgaagaaggg atggcacgcg gcgcagatgc cgacgcgcag gttctgcacg
    30361 gtggagatgg tgggatagac cgcgccgcag gtgcaggtga tcgtggtttc gtaggtggtg
    30421 gggtggcctt gttctttcat ggtgcgaagg ctcacgggat gtccgtcgag gggcgcgggc
    30481 cgctttcgcc ggggcgtgag ggccgccatt ctggcatagg cggcaacacc gtcaaccgaa
    30541 tatctccagc cgcaaaaatc cctcattccc ggtttgtgac gcgatgccgt gacgtgaaaa
    30601 tcaccgttgc cgcgcgtttt ctttgaaaat gtttcgtgcc agcccagccc tcctgcttgc
    30661 cgccatcccc ttgtatgccg cgcctgactt tgagcgcgag gtggccccca ttctcgaagc
    30721 cagctgcctc tcctgccacg acgccgccaa cgccaagggt gacgtccgcc tcgacaccca
    30781 ggccgccgtc ctggagcacc tcaagcccgg ccttcccgac gccagcctgc tcatcgagca
    30841 agtcagcggc cctgagccgg acatgccgaa aaaagaaaaa ccgctcaccc ccgcccaagt
    30901 cgaaaccctc cgccaatggg tgcaatccgg cgccgtctgg tccgaggggc gcgtgcttgc
    30961 tgacaaaccg gcgcgtgatc ttgattggtg gagcctgcgg gaaattcgaa aaccgaaacc
    31021 cgaagctcga aacggcaatc cggtggattc cttcatcgag gccaagctcg ccgagaaggg
    31081 cctcaagccc gtccccgaag ccgaccccgc caccctcatc cgccgcctca gctacgacct
    31141 caccggcctg ccgcccagcg cgcacaactt cgaatttcga gcttcaagtt tcgattttga
    31201 ctccctcgtg gaccgccttc tgagcagccc cgccttcggc gaaaaatggg cgcagcactg
    31261 gctcgacctc gtccgctacg ccgagaccca cggctacgac aaggacaagc cgcgcatgaa
    31321 cgcctggcct taccgtgact acgtcatccg cagcctcaac gatgacaaac cctacgaccg
    31381 cttcgtgcag gagcagatcg ccggcgacgc gctttttcca ggccagcccg atggcgttct
    31441 cggcctcggc ttcctcgccg ccggaccgtg ggattacatc ggccactccg aagtcggcga
    31501 gggcaaagtg gacggccgca tcgccaagca cctggaccgc gacgaaatgg tctccgctgt
    31561 cttcaacatc ttcaccagca ccaccgtcca gtgcgcgcag tgccatcacc acaagttcga
    31621 ccccgtcacc atgacggact actaccgcct gcacgccgtc ctcgccgccg tggatcgcgc
    31681 cgaccgcgtc ttcaccggcc tgccgcctga aaaggaaaag gagcgcaata cgctgctcgc
    31741 ccgaatcaac gccctcaaag ccgagcaaac gaaactcacc accgacgcca agcgcgaact
    31801 cggtgcgcgc acctcaggca tcgagcgccg cctcgccgag ctcaaacaaa agcacggctc
    31861 cccgctgcgc ccgcagtatg gatggcacag ccacatttcc aaaatccagg ccgccgaaaa
    31921 gtgggtccag gtggacctcg gcaaggcgca gggcgtcgag caagtccgcc tcatccccgc
    31981 ctacgacgac tacaacagca tcggcgccgg tttcggcttc cctcttcgct acaaggtcga
    32041 ggccggcaac gaccccggct tcaacaccgg cgtccgcctc atccacgacg ccaccgccgc
    32101 cgaccagccc aatccccgca acagcgagat cgtccttgat gtgggccgcg atgctgtgcg
    32161 ctacctccgc gtcaccgcca cccgcctggc cccgcgcaag aacgactaca ttttcgctct
    32221 cggcgagatg caggccatcg gcggtgaaaa ccacgacaac atggcccagg gcgccgccgt
    32281 caccgcgctc gacagcattg aggccgcccc gcgctggggc cgcgccaacc ttaccgacgg
    32341 catctaccac cgcgaagtcg gcgaccccgc cgcgctcgtc gagatgcgcg agcttgaagt
    32401 tcagaaagcc gccattgaaa aggaactcgc cgatcctcgg cgcgacgccc gccttgacga
    32461 gatcgaaaaa catctcgccg acctcaaccc caagctcgcc acctacccca aaggtgaact
    32521 cgtttatgcc gcctcaaccg acttcaccgc gcagggccgc ttcatcgcca ccgccggcaa
    32581 gccgcgcgaa atccggctcc tgaaccgcgg tgatatccgc gcccccggcg gtctcatgca
    32641 acccggcctt cccccgctct ggcccggcgc gcccgagcag ttcaaccttc ccaccgaagc
    32701 ccccgagtcc gccgcgcgcg ccgagctggc caaagccatc accgcgcgtg acaacccgct
    32761 cttctggcgc agcatcgtca accgcctctg gctctggacc ttcggccagg gcctcgtcac
    32821 cagcgccaat gacttcggcc gcatgggcca gccgcctacc caccccgagc tgctcgacca
    32881 cctcgccgcc accctgcgcg atgaccctca gcactcgttg aaaaacctcg tccgcctgct
    32941 cgtcaccagc cgcgcctacc gacgcgccag cacccatcac gaagccaacg ccgcccttga
    33001 cgccagcaac gcctgcctct ggcgcgccaa ccgccgccgc ctcagcgccg aggaatatcg
    33061 cgacagcctg ctcgccgtca gcggtgtcct gcggatggag gatcgcggcg gacccagctt
    33121 ccaggatttc gtcatcgaaa aaccgcagca ctccccgcat tatcaatacc acctccacga
    33181 tccgcgcgac ccctccgccc accgccgcag catctaccgc ttcatcgtcc gcagccagcc
    33241 gcagcctttc ctcaccagcc tcgactgcgc cgacccctcc caaagcgttc cccagcgcga
    33301 tgaatccacc accgccctcc aggccctcac ccagtggaac aatcgcctcg tcgaagccat
    33361 gagcgacacc ttcggccagc gtctcgggca ggaaaaagac cccgtcaacg ccgccttcac
    33421 ccgcgccctc tcccgccccc ccaccgccga agagcgcacc gtcctcaccg cccatcttca
    33481 agcccacggc cccgccgccc tcgcccgcgt tctctacaac ctcaatgcct tcgtctattt
    33541 ggattgacac catgaaccgc cgtcagttcc tcacctccct caccggcctc gcgctgaccg
    33601 acctgctgct gcgggacgcc gcgctcggcg cgcccggcac gctgcgcggc gggctgcatc
    33661 atccgccgaa ggcaaagcgc gtcatccagc tcttcatggc gggagcggcc agccatgtgg
    33721 acatgtggga ccacaagccg ctgctcaccc aaaaagacgg ccagccctgg gaccctggag
    33781 agaaggtgga gctctttcaa agcacccccg gcgcctgctt cggcagccct tgggccttca
    33841 aaccgcatgg acagtccggc aaaatgctca gcgagatcgt ggccccgctc ggcggcgtgg
    33901 tggacgacat cgccttcgtt cacaacgtcg tcggcaagac cggcgtccac agccagggca
    33961 cgctgctgca aaccaccggc ttccaactcc caggcttccc cagcgcgggt tcctgggtca
    34021 gctacgcgct tggcagtgag agcgacaacc tgccctcttt cgtcgtcctg ccggaccacc
    34081 gcggcctcgc ctccaacggc gccaaaaact gggccagcgc cttcttgccc gcgcagcacc
    34141 agggcaccgc catccgcccc ggcagcgccg aacccgtcgc cgatctgctg ccgccgcaga
    34201 gcgagttcat tacccccggc tccgaccgcg ccgggctgga tgtactggcc aaactgaaca
    34261 ctgcccacgc gcaagcgcgc cccggtgatg accgcctgct ggcgcgcgtt cgcagctacg
    34321 aactggccgc cgccatgcaa ctcagcgcca ccgacgctct ggatgtctcc aaggagccgc
    34381 ggcacatcca ggacctctac ggtctcgcct ccgaaggccc cggcgtggat gacaccacga
    34441 tcaacgtcaa agccgagacc gaattctttg gccgcaaatg cctcgtggcg cgccggttgc
    34501 tggagcgcgg cgtgcgtttt gtgcagatct ggagcggcaa cgacaacggc ttcccccggc
    34561 gcaactggga cagccacgag gacgtgaagc gcgaccacga gccgctctcc ctcggcatgg
    34621 cgcatggcac ggcggcgctg atcaaagacc tcaaacagcg cggcatgctg gaggacacga
    34681 tcatcctctg gaccacagaa ttcggtcgca tgccctgctc ccagggcagc aagggccggg
    34741 accacaaccc cttcgtcttc accaactggc tctgcggcgg cggcatcttg ggcggcacct
    34801 atggcgaaag tgatgaatgg ggattccgcc cgcaggacac cgccaatgcc accaccgccc
    34861 atgacatcca cgccaccctc ctgcaccagc tcggcatcaa ccacgagcgt ctcaccgtcc
    34921 gccacaacag cattgaccga cgcctcaccg acgtccacgg ccatgtgctc aaggcgatgc
    34981 tagtgtgagc cgcatctttc ctctcgtctc caggcgggac gcggctaaaa tggccgccca
    35041 tgtcccagcc tgcaaatccg agtccgcacc agccccccgc cgcgggcgcc tccgccgcgc
    35101 ggttgctcgc actcgcggaa acaggacgtc cagattccta ttaccggctg gcgcgcatgg
    35161 tggcgcgtgt tttgaccagc caaagcctgc gccgccgccg caaatggtgc gagctggcgc
    35221 atgcactcgc cgcctcatca ggagtcgagg cgcgtctcat catgcaggcg ctgcggcgct
    35281 ttgatcccgg cgcagccgca aacttccctg tgaaatcatg agctgggaaa ctccaccccc
    35341 ggtgtgggac acgcccgcgg gcagaatgct ggatgccttt ctggcagcgc taaaaaagga
    35401 cgcgcccggt tacagcgaac ccctcaccat tttcggctcc gccgccatcc agctctgcct
    35461 ggacgagtcc ttcaccagcg ccgatgtgga tgtgatggcg ctcacaggaa acgagcgcct
    35521 gcgcgagatc gcaaagacgg cgggcttggg cgccacaggc agcatccgcg ccgcctacgg
    35581 catccagatt tgcccgccgc aactgttccg caccacgcct cattatctgc ttcgggcgcg
    35641 tatggagcgc agacatggca tcaaagtcat cctgccgcat ttgcgggatg tgctgctggc
    35701 gaagctgcac cgttcccgcg cgcctgacca ggaaggtctg gcgcccaagg acaggcgcgc
    35761 cttccaacgc gtgcgcgaac tctgtggcgg tcatcccacc gaggcggatg tcattgagga
    35821 tctccggctt tgcagccagg atctcctcat ccaggcagcc gaagagccga atgccttccg
    35881 cctgaatgtg ctcgatttat ttcgctccgt gttcggccgc ggtttggaca tcgagaatga
    35941 aattttaaag cccgcacgcc aagccctcgg caccgttcag gatgtggatc atgaggaaat
    36001 ccagcggcgg ctggcaagtc tgcgccccga ccgtgattga agccgaaggc tcactccccg
    36061 tgatgagaac cactggcaaa cacctccccc gcccttttca gttcctcgac aaaaacggcc
    36121 agcactttgt ccctggtctg gcgcgtggtg acgcctgcgg aaatgcagac gggcgcgggt
    36181 tgcggctgga gtgtcttcaa gacgatgcgc tccggcacga ggcaggcgat gcgctccacc
    36241 acgagcgcca cgccgaggcc tgcctccacg gcgctgatga ggctggtcac gccgtcgcat
    36301 tcgctggcga cgcgggcttt gatgccctgg cttttgaacc agcctgaaag cagcgcccag
    36361 tagtcgggat actcgcgctg attgtaaacc acgaggcgct gtccatcgag atcggcgggc
    36421 agcagggtcg ttttgccggc cagggcatgc tggcgcggca cggccacgca ccagcgctgc
    36481 ttctgcacgg gcgtccaaac catctccggc acctctttgc cgggcggcac agtgacgatg
    36541 acatcgagcg cgcctttttg cagtccctcg accatttcgg tcgaactgag atcgctcagt
    36601 tccacgcgcg ccttcggatg aagctgggag aaggcctcca tcgccggcgc caggatgccc
    36661 gctgtgaggg agggcgaata gcccacgcgc agcgtctgcg cccgcgccgc cgccttgatg
    36721 cgctgctcca aatgatccgc ccgctccagc agcgcgcgcc cctcccgcag cagcagctcc
    36781 cctgaggccg tcaaagaaaa cgaatgcgcg ccacgctcca gcagcggcac cccgagatca
    36841 tcctccagcg acttgatctg ccggctcagc gcgggctgcg tgaggctcag gcgcgcggcc
    36901 gccttgctga tgttgccatc ctcggcggcg gcgatgaagg agcgcaggag tcggagttcc
    36961 atgagcggtc agccagtcac gacttcgggg tattgctggc gcaggaagat caggtcggga
    37021 atgtccttct cgcggtgagt tttgaatctg taaaccccgc gcggacaaag aaagtgcccc
    37081 tggctggcga aaaactggtt tcgagtctcc tgcattccgg caggggagac aggcggcttg
    37141 cgccgtccca cggtcttgcc gataggctcg tccagattga tcacggttcg cggctgcatg
    37201 gtgaatggta gagcctgacg catcatccgc caagcctcat cctgcctgcg ccggggtctc
    37261 caggcttggg gcgtggaatg ttgatcacga ccccatgcgg ctccggtgct cgctggggcg
    37321 ccagccgatc cactttttga aggtgttgct gaagacgaag gggttttggt agccgacggt
    37381 gtgggagacg gtctcgactt tgaggttggt ggtggagagc agctcgcagg cctttctcat
    37441 gcgcagccag gtgacgtggt gcatgggcgt gcggccgagg gtcttgcggc agaggcggcg
    37501 gaggtgctcg gagctgacgt gggcttggtg ggcgagttcg tcgagcgtcc agtcccggcc
    37561 gacatcggcg gccacttttt cccacacgcg ccagaggcgg tcgtcctgct gccagggctg
    37621 ggcgaagcgc tcgacgtagt tttgaatgag ctcgacccag tggaacatgg cggcgggctt
    37681 ggcctcgtgc gcggcctcct cgatgaggcc ctcgatggcg ctccacagcg gcctgccgtg
    37741 gaaggcggcc atctgcgggg agctgctgct gaggatgggg cgctggccgg gctcctgctc
    37801 atagcgcacc cagcagcagc gccaggcttt gcctttgacg gcctcgaagg cgttcaccat
    37861 gaaaggcggc agcgccacgg ccatgcccga gccgcatttc tgccagcggc cgtccaccag
    37921 cacgcggccc tccccctcca ggcaggccag gaaatacagg ccgccctggt gcatgcgcac
    37981 gatgcggtag ggcgcgcgcg ccgtcatgac gccggcgtgg gaaatacggt gcgcgcgcag
    38041 gctgccgcaa accggagcac cgcgcagcca ggggcggcgg tcggcctcgt cggcgcgcac
    38101 cacgcgatag tcggtgtccg ggccgtgggt gtgcgtttcg gatagt
//
LOCUS       DAOMYJ010000025        37592 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Prosthecobacter sp. isolate AMR_MDS_5629
            NODE_415_length_37592_cov_20.787650, whole genome shotgun sequence.
ACCESSION   DAOMYJ010000025 DAOMYJ010000000
VERSION     DAOMYJ010000025.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867850
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prosthecobacter sp. (wastewater metagenome)
  ORGANISM  Prosthecobacter sp.
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Verrucomicrobiaceae; Prosthecobacter.
REFERENCE   1  (bases 1 to 37592)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 37592)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.30
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:34:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,742
            CDSs (total)                      :: 4,684
            Genes (coding)                    :: 4,676
            CDSs (with protein)               :: 4,676
            Genes (RNA)                       :: 58
            tRNAs                             :: 55
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37592
                     /organism="Prosthecobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_415_length_37592_cov_20.787650"
                     /isolate="AMR_MDS_5629"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1965333"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(99..257)
                     /locus_tag="PLP58_04435"
     CDS             complement(99..257)
                     /locus_tag="PLP58_04435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ07791.1"
                     /translation="MDEDFRFLDRDTDFDHTIKGLTPGAVLHAYVVAANPAGEAAPSP
                     VVTKVVGV"
     gene            638..12646
                     /locus_tag="PLP58_04440"
     CDS             638..12646
                     /locus_tag="PLP58_04440"
                     /inference="COORDINATES: protein motif:HMM:NF012200.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choice-of-anchor D domain-containing protein"
                     /protein_id="HRJ07792.1"
                     /translation="MNTRSSLLCLRRALLAPALGLLGLGGTLAADAHDAPAAPAAIPF
                     SQLGAEADKQGAQAASSITPTATGAHLKALMQDLEAEAAPDGLWLTSTADEDAGKPHR
                     FRVRALSLGRADCPQSAASVSPAALGGPSALPQSGAVRASAESALWLRPGLVEEYRVS
                     TDGVRQDFIVMQRPAGAGEELAVDLEVTGARAETAAYGVKLIVSGTGRELAYSRLKVT
                     DAAGKELTARLEVVDAGRLRVLVRDAGAAYPVRIDPTFSDADWVALNPSIPGATSSVQ
                     VITVDGSGNLYAGGDFTAIATVAANRIAKWNGSAWSALGTGMDSTVRALVVSGGDLYA
                     GGDFSTAGGISASRIAKWDGSAWSALSTGMDGSVQALAVSGGELYAGGNFTTAGGTSA
                     NYIAKWDGSAWSALGLGMNASVRALAVSGGEIYAGGQFTMAGGITVNRIAKWDGTVWS
                     ALGTGMNNLVLALAVSGGDLYAGGGFTTAGGVSGRNRIAKWDGTAWSALGTGMSSTVQ
                     ALAVSGGDLYAGGFFTTAGGTTVNRIAKWDGTAWSALGTGMNNTVLALAVSGGDLYAG
                     GSFTTAGGTGIGYGIAKWDGSAWSALSTGMDGSVQALAVSGGDLYAGGDFTTAGGTSA
                     SYIAKWNGSAWSALGTGMNGNVYALAVSGGDLYAGGLFTSAGGTTVNRIAKWDGSAWS
                     ALGTGMDSYVNALAVSGGEIYAGGGFTTAGGTMVNRVAKWDGTAWSALGSGVSATVNA
                     LAVSGGDLYAGGSFTSAGGTSIGYGIAKWDGSAWSALGSGMDNVVHALAVSGGDLYAG
                     GDFSTAGGTGANFIAKWDGSAWSALGTGMNTSVRALAASGGDLYAGGDFIMAGGTSAV
                     RIAKWDGSAWSALGSGVSNVVRALAADASDHLFVGGAFLTAGATFTPYIAQANLNGGS
                     GAPEIALSGNGQDIENGDDTPDTADHTAFGSVAVAGGSVARTFTITNSGAAVLNLTGT
                     APDYVTLSGSSAFTVTTQPAAATVAASGGTQTFVITYDPSAVGTHTATVSIANADGDE
                     NPFTFDISGTGLNSTPTDITLTPASIAEENAPNATVGVLDAVDADVADTHTFTLVAGT
                     GDTDNGSFTIAGTDLKLTPVADFETKNSYSLRVQADDGAGGLFEKALTVTITNIYEPV
                     GGAQVTFVNPAGGDFRIQSITGLTVAGTVPGTLWDFTYYHGVDYASVPGPHLAAEGLI
                     DHINAGHAVVNFFAAAGIDASTGTAFTVRGNLPIAAPSGGNVTVNGFNRSSSAPHNPL
                     TYGQACCYNPSDTAALPADQAWVVITLQPEPEIAVSGNSVNIADGDTTPDAADHTDFG
                     TLAVAGGSVARTFTITNSGTAVLNLTGTAPDYVTLSGAGATHFSVTTQPASATVAASG
                     GTQTFVITYDPSASGAHTATVSIANDDGDKNPFNFDISGTGNSAPTDITLTPASIAEN
                     NASGAVVGELDAVDADAADTHTFTLVAGAGSTDNASFEIDGEDLKLNAVADFETKASY
                     SIRVQADDGAGGLFEKALTVAVTNEIEAPADLDPTFDTDGRVVADLGGTEAANCVAIQ
                     ADGKIVVAGTSNASGSGDFMVARYLPDGTPDTSFGTGGVVLTDVSGAGSADLGRRLAI
                     QTDGKIVVVGESNAGTGGLNFALARYMPDGTLDSCFGTGGLVVLDGGGGMDDGLNAVT
                     LQADGKIVAAGYSWSSFSGGTRTAVVLRFLATGALDPSFGTGGSWGLNFGGSQYSAGG
                     VAVDGSGRIVVAGFGPQPNWNGFVARLTAAGSTDTSFNGGVQQANFGAFTESGFNAVR
                     LQSDGRIVVAGNRSNGSYGPTTDFALSRYLENGTLDTSFGTAGMTTTDRAGRLDNIIS
                     ITLDVHDRIIAAGIQQDAAGVEVFGINRYTRNGILDTTFGTGGRVLTTFGAVTGNRAA
                     WTALDADGRIVAAGFAGNDIALARYEGGPAAPEMVVTGNSTLIADGDATPATADHTDF
                     GSALAAGATVARTFTITNIGDAELNLTGTPLVEVSGTHAADFSVTLAPATPVAEEGGS
                     TTFQVTFDPGGLGLRTATISIANDDEDENPYNFSIQGTGLNSTPTDITLTPASIAENN
                     VSGAVVGELDAVDADAADTHTFTLVAGAGSTDNASFEIDGEDLKLNAVADFETKASYS
                     IRVQASDGNGGLFEKELTVAVTNVNEAPSLASSYLVYETVTPTRSSNNIVYSVNNAAA
                     LAGTPFTRVRYRMETRVGGTLRHADVSFDKWSGLTVAGLAVPTPNNPFIVQRNVTNMN
                     VASNWSGQGNATAVTTGSGFTGRLEIWERNYGTANVFGDGNGSLYDFDDTPSAGGYGC
                     FQVHNVSSPTKHTVLAWNNHGSTTPDIGFGNQIGGSGHPDWTFAGGSSLGKTDWKLQI
                     YVTTEPVLSLPENSAAGTLAGTLAGTDPDAGNTLTYSLVSGTGDTDNGSFTISGNQVL
                     SDVVFNYETKSSYNIRVRVTDNGGLFSESALTVTVTNVNEAVLSLALTGSSVNENVPA
                     NTTVGTFSSTDTDAGDTHTYTLVSGTGSTDNGSFNISGSTLRANAGLNYEAGATRSIR
                     VRSTDAGGLFAEDIFTITINNVNEAPTISDIASLAINEDANTGALAFTIGDPDAGASL
                     NVSAASGNTTLVPLSSIALGGSGTDRTVTVTPAADLHGSALITVTVSDGALSAQDTFT
                     VTVNSVNDAPSFTISLPGGSDLAVSTLAGSPGLSGSTDGTGSAARFNNPWGGVSADDA
                     GNVYVADQANHIIRKITPGGVVTTIAGSAGLTGSANGTGAAARFNYPNAVVVDPAGTE
                     LFVVDLVNNTIRRVDLADNSVTTFAGTAGLAGASNGTGAAARFNYPIGIARDSDGNLY
                     VTESNNNTLRKITPGAVVTTLASFFNNPYHPAVDSAGNVYVPQPNRHTIIKVTPAGVK
                     SVFAGTDGSSGAADGTGAAARFNIPTGVAVDSDDNVYVADYNNHTLRMITPAAEVTTV
                     AGVAGAAGSTNGAASVARFNKPLSLSFDGAGDLYLADSASFTVRKLSADLAPLVVAED
                     SGAYSGAAFLTAISPGPANESAQTVSFTVTNNNNGLFSAQPAIAPNGTLTFTPAPDQN
                     GQATVTVTAVDDGGTANGGVNTSAPQTFVITVTPENDAPVITSDGGGDDASLNVAENS
                     TAVTTVSAADIDLPAQTLTFNITGGDDSALFSIGSATGVLSFLNAPNYESPADADLDN
                     VYELIVEVSDGNGGTDEQEISVTVTNVNEAPFITYGGTTPPSVWNMPENQVDLIPFEA
                     DDPDFETVTLTFSIHGGADQARFGIDAETGELAFLATPDFENPADADTDNVYHVVIAV
                     TDDGTPALTTTHAVSITITDTNDAPVIISNGGGALAAVNVPENTTAVTTVVATDDDLP
                     AQTLTFSIIGGADAARFTLGGGTGVLAFNSGKDHEFPSDQNGDNVYEVVVRVEDDASP
                     PSSDSQTILVTITPVDEAPLAWTHGNEAPTTSSAGVFGEALPNGLDTSAWFEYSTDPA
                     VETGVLTTAAQTLGSASVLVDVTGTLTGLTEGTTYYFRLVAQNSLGMVKGEILSLHTQ
                     SLFNGTAPLAGGITIKRPYPGPINEAGQVVFKAQATIKSGLVSSANDMLLVTNSSGTL
                     GIIAREGTVITGGARFTGLFNHPLVTEGGQALALEKLANATTVNDYLYPVSEDGVSLD
                     QLSREGDSAPDSDGGVFLGHANRPAADGQDRVYFSSSLTGVSTKKDSGIWYDDGGVLT
                     KLVLEGEKAPAGDTTALQAWYGNIQSMVAAGGDGVAFIAALQGNPGDTTQKTSTAMNA
                     GVFAGPPGDIAIIARKGGVITGTGTLNAFNAVSRSSTGGHAYLATLKNSSVAPVVTTA
                     NNQVLVIKTGTLENIVARKGVTVITGALTPSKFGNFTITDDDEVVFQVTLAGTGVTTA
                     NDQALCRWTEAGGIELLAREGSAAPGIGANYLAFLAFSVSPSGNIVLHSTLSGTGASA
                     LLRDTGSGLALLVRNGSLVPHGTTQRAVKAMSIHTGYGDGGGAGGMGAAINDNGEVFT
                     VLDLSGVYQAKVFP"
     gene            complement(12674..13579)
                     /locus_tag="PLP58_04445"
     CDS             complement(12674..13579)
                     /locus_tag="PLP58_04445"
                     /inference="COORDINATES: protein motif:HMM:NF014376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HRJ07793.1"
                     /translation="MPATEQADYFSTQVLRTRRFYLPDWRRRQREKLGLCLVGGGCEW
                     CAPDFVVDRQSLPFHAFEFVGRGKGDVTLAGRKQEIGTGHAYFFDPGTPHLIRSGSED
                     PLVKYFFNFAGVRAAALLEELGLGPGAVIRVLDAGRVTALLEEVIDHALRGGRLGLRA
                     AAATLEHALVLCADSRQPAATKLDPAYATYLRCRGHLLRNYPVLGSIEEAARHCHVSA
                     AYMTRLFKRYDSETPLSCLTRLKLSQAAIKLRQPDAQVKAVAWELGYKSAAHFSRVFK
                     EWSGAAPLHWQRGEGGAGFEERAKR"
     gene            13708..14622
                     /locus_tag="PLP58_04450"
     CDS             13708..14622
                     /locus_tag="PLP58_04450"
                     /EC_number="4.1.3.3"
                     /EC_number="4.2.1.41"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009962480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009436 - glyoxylate catabolic process
                     [Evidence IEA];
                     GO_process: GO:0071704 - organic substance metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HRJ07794.1"
                     /translation="MQPPQPLRGIIPPLVTPLRDRDTLDTAGLERLVEHLISGGVSGL
                     FILGTTGEGPSLSYRLRRELVQLACKFANNRVPVLTGITDTSFTESVNLARHAADCGA
                     THVVTSAPYYFPAAPPEMLEYVQDLVAEMPLPLFLYNMPGLTKVSFELDLVRRALDMP
                     GICGVKDSSCDMIYFHRLIEVARQRADWSVLVGPEELTAEAVLLGGHGGINGGANLHP
                     RLYVDMYHAAAAQDLQRTRELHAQVMSLAGAIYTVGRHKSAIIKGIKCALSLTGVCED
                     HMAEPFHRFREPERGLMRERLMAIGLLA"
     gene            complement(14653..15867)
                     /locus_tag="PLP58_04455"
     CDS             complement(14653..15867)
                     /locus_tag="PLP58_04455"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009962725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent RNA methyltransferase"
                     /protein_id="HRJ07795.1"
                     /translation="MPPRKFQPHPFSYHQELDLVIENLANEGRGVARVDGWVVFVSFA
                     LPGERVRARVFRNHKNYSEADLVEVIEPSPDRVEPRCALFGQCGGCQYQHLAYDRQLE
                     WKRSQVGELLWHMARIKHPVLPVIPSPRQYGYRSKITPHFQKPKNGEIGAIGFLRAGT
                     RNAMVDVARCPIAMDALNAELSRAREAARAVPGTFKNGATLLMRAAANGVLTRPDETA
                     VEDVGGVRFEFHAGDFFQNNPFILPEFVSHAVREAAASGARRLVDAYCGSGLFAISAA
                     REFQEVIGVEISEGAVRKAAHNAEINGLTHCRFLAADAAAIFESIEGGGSETVVIIDP
                     PRAGCSEDFLRQLFAFAPRRVVYVSCNPATQMRDLALFTEAGFNLLKVQPFDLFPQTR
                     HLECVMTLEKSW"
     gene            15944..17371
                     /locus_tag="PLP58_04460"
     CDS             15944..17371
                     /locus_tag="PLP58_04460"
                     /inference="COORDINATES: protein motif:HMM:NF013132.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008509 - monoatomic anion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006820 - monoatomic anion transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DASS family sodium-coupled anion symporter"
                     /protein_id="HRJ07796.1"
                     /translation="MLDTRNEIHRLMRRFEFDSGRAVLKFSLCALAAYAAAAWPEHPG
                     LGDAGRCSLGIVMLGAGLWITEAIPAFAVALLVIGLQIITLGREGGVLAETGDSKAWE
                     DYVRPWSSPPMWLFFGGLVLARAAERTGLDRHVAGRVLAWTRGSPSRLLPAVMALAFV
                     FSMFMSNTATAAMMLAMLRPALASLPEGSKTARALLLGLACAANLGGMATIIGTPPNA
                     IAAALLEDDAPVDFLRWMFLALPPALLLFAVVWALLARPLIREKSVLPPLQEAPREGS
                     GVRRWQRLLTLAVFAVTVLLWMSGEWHGIPTGVVAFVPIVALSMAGVIRKQDMRAIDW
                     DVLILLAGGLSLGVGIEKSGLAEWLAGLVQQAEMPSWQASLVLCLIAGGISNLMSNTA
                     AANILLPVTLVIAQAMGGDADVTMFVVPVALACSTAMALPISTPPNAIVYASGRLRGT
                     DYLAPGLLTLVLGPVLALGWCMIAG"
     gene            complement(17378..19213)
                     /locus_tag="PLP58_04465"
     CDS             complement(17378..19213)
                     /locus_tag="PLP58_04465"
                     /inference="COORDINATES: protein motif:HMM:NF019223.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1549 and DUF1553 domain-containing protein"
                     /protein_id="HRJ07797.1"
                     /translation="MKALPVILASLVLHDAVHARAIRASADPVRDEPPITEDERGHWS
                     FQPLAFPVKPARSGQARGASAELDGLIEKRLLEAGLTRSDEADAATLIRRLSFDLTGL
                     PPTPEEVDAFIKTCARDKDTAIHALVERLLASPAYGEHWAQWWLDLARFAETDGFEHD
                     KERPRAWQYRDWVIRALNEDMPFDEFVRQQIAGDLLDGGEPAATGFLFSGPDMPDLNN
                     QDERRHVVLNDITSTTGSVFLGLTVGCAQCHDHAYDPLSQADFYRLRAFFDNTVLTAR
                     DKPLGPQVRVFTEGVPASTVFVRGEFRRPGPVIQPGYPRIAGGLDQPAGDRAALARWI
                     TRADNALFLRATANRVWQQHFGRAIAPTPADLGRQSPPPTHPELLDWLAAELPRQGWS
                     LKRLHKAIVLSQTFRQATPPPGMSGEAIGLYAAMPRKRLTGEMLRDSLLVITGQLNAR
                     AGGPGARLPLPAEISGTLLKKQAEATVDESELRRRSIYAFARRNARHPIFDLFDRPDA
                     LVTCSRRETSTTAPQALLLMNSELTQGIASTLAGQALAACGSDMGAIADFAVRRCFSR
                     AASAAERDAARQFLDGQTALAPAFQDAVADYCLALINSNEFVHVD"
     gene            complement(19217..20707)
                     /locus_tag="PLP58_04470"
     CDS             complement(19217..20707)
                     /locus_tag="PLP58_04470"
                     /inference="COORDINATES: protein motif:HMM:NF019040.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1501 domain-containing protein"
                     /protein_id="HRJ07798.1"
                     /translation="MTVEADGRLSQARECSMPQYRLKGRDLARLSVMSPATPPFVSRR
                     RLLGGAAAVLPGVAFSHLLAAEHDPAGAAHFPAKAKRVIWLFMHGGPSHVDLFDPKPE
                     LVRHAGKPLPESLGAVETRRKVAENPLLGPVKPFRRHGAGGIEISGFLPRMAELADEM
                     CVIRSCHGDSVNHPQSVYQMNTGSILMGKPSLGSWVSYGLGSENADMPAFVVMPDPGG
                     GLKGGPPAWGSAFLPATHQGTTLRPGENPILHLRPAGGQSSARQRSTLDLVNRLNQAH
                     QSARAEDTHLDARMRAYELAFRMQSAAPEAVDLSKESDATKTLYGLDQPVTREFGTRC
                     LLARRLVERGVRFVQLYSGDTNGWDAHEDVLKNHTDYSARTDQPVAALILDLKQRGLL
                     DDTLVIWGGEFGRMPMSEKGTGRDHNPWGYTTVLFGAGVKRGHVHGATDDFGLRAEVD
                     KVHVHDLHATILHLLGVDHERLTHRHNGRDERLTDVAGNVVRGILA"
     gene            20762..21229
                     /locus_tag="PLP58_04475"
     CDS             20762..21229
                     /locus_tag="PLP58_04475"
                     /inference="COORDINATES: protein motif:HMM:NF024965.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HRJ07799.1"
                     /translation="MASITTQELSDHALWLESSGAQGRRIDETGLNLSGHDLAGLNLA
                     RAQFVGTVFDGADLAGTNLSGADFSGATFEGANLTGADLGGADLGGVTLSGANLSGAN
                     LRGADLSGARLDGADLTGADFTDADLIAADLTGAKQGGMILAGAKLNDTRGLE"
     gene            complement(21239..22195)
                     /locus_tag="PLP58_04480"
     CDS             complement(21239..22195)
                     /locus_tag="PLP58_04480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ07800.1"
                     /translation="MNLREQLRILLPEILPDDPDQAIKGTELIRMVRLRLAGDYSDAT
                     LRYHFSILSYDSTSPIAKVDQGQGYYLRQARRAPDSTSEAYLSFAAGPEGDARVARFL
                     RLLAIYERRSLPLAQHPFQLNGRGREELEVRGHWDIPDLVIAEWDLETGADDNVTRFD
                     SAMINLRRHLGGPEAGITGVQIKSGVTLENCRAEFFQTLSATSWTLQSEMVVAEPLND
                     EALVRALRSLGSQFGVGISSLGINTAQLDELPSAADITRMSAAEFDSVLAKLRPQRVT
                     APQLRQRMDWTALSSLREKHASVEELIRWLGGSLAAGRPAER"
     gene            complement(22249..22962)
                     /locus_tag="PLP58_04485"
     CDS             complement(22249..22962)
                     /locus_tag="PLP58_04485"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009964012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HRJ07801.1"
                     /translation="MSSAPALSVVVPLYNEQDNVARLQEEIAAALRGLDYEVVLVDDG
                     STDGTVARVRPDARTRLVEFERNAGQSAAMHAGIHDARGAVIAMLDGDLQNAPADIPA
                     MLNRLDEGWDLVCGWRVSRQDSMFKRLQSRVANTVRSRFVRDHVHDTGCTLKVMRREC
                     REALLPFNGMHRFIPALVAAMGWRVTEMPVSHRPRLHGASKYGFGNRAWRATMDMFGV
                     RWLNSRRVVARRKDSSFPS"
     gene            complement(22959..24020)
                     /gene="lpxD"
                     /locus_tag="PLP58_04490"
     CDS             complement(22959..24020)
                     /gene="lpxD"
                     /locus_tag="PLP58_04490"
                     /EC_number="2.3.1.191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018291930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-(3-hydroxymyristoyl)glucosamine
                     N-acyltransferase"
                     /protein_id="HRJ07802.1"
                     /translation="MSSSIPLNKLAELVGGEILRGDPRTPISGLNSIVEAQPGEVTFL
                     GNDRYAEALATTRASAVLVARSARLACGMAALIAVENPTLAFSTVIRHFIPAAAPAKP
                     GVHASAVVAPGVSFDAGKVHIGACAIVEEGASLGDGCVVHAGAFVGKGASLGPSCVLH
                     PNSTVRERCVLGARVIIHAGAVIGSDGFGYEFTQGRNVKVEQVGIVRLDDDVEVGSCA
                     TIDRARFGLTWIGEGTKIDNLVQIAHNVVVGKHCVIVSQVGIAGSTHVGDCVTLAGQV
                     GVAGHLEIGSGVTVLAKSGVTKSITEPGVYTGFPARPLIEGRKLMALPAKIPDMLARL
                     RELERRLSEMESAAKDASS"
     gene            complement(24085..24696)
                     /locus_tag="PLP58_04495"
     CDS             complement(24085..24696)
                     /locus_tag="PLP58_04495"
                     /inference="COORDINATES: protein motif:HMM:NF015871.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpH family outer membrane protein"
                     /protein_id="HRJ07803.1"
                     /translation="MLRFSILILTSAFLTSAPAADLKFAVVDMSKAFSEFQKTQEAAD
                     KFKGNVEKAQKEMNDRWAVYKGLMDTMQKLKKEISDPISSPDVRNKKAGEFEEKAREA
                     RALEQEITEAQNRRTNQLKQEDVEIRRDLYAEIVAVVRDHAKAQGFDFVFDKTGASLS
                     TVPVLLYYKDAADITDQVIVELNKKRDAAPAADAPAGAAAGKP"
     gene            complement(24732..27128)
                     /gene="bamA"
                     /locus_tag="PLP58_04500"
     CDS             complement(24732..27128)
                     /gene="bamA"
                     /locus_tag="PLP58_04500"
                     /inference="COORDINATES: protein motif:HMM:TIGR03303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamA"
                     /protein_id="HRJ07804.1"
                     /translation="MISGVFSRASLLMRPWLAVLITTSALTAQGQVSLDTPAASRGGR
                     IVQAVDVQFQGAAALDPDRVRAQMSTRVGEPYSDEAVERDLRNLYQTGAVENVDIQAV
                     NVSGGVRVVVTIYGRGGIGEIGFVGNAAFDNDKLRKEIEVKVGDPVDEIKLAAAQQKI
                     LDLYTKKGFSDALVTYETQPSTKPGFTSVLFKIEEGGKGLIGDIRFEGNSAIGDRKLR
                     SALTSKEKTFWRLWGKAGKLDNQAVLEDVRKIETLYQDQGYVYVKVGYRREVVSSDRV
                     ALVFEINEGGQYEVADVALEGVTLFSKDELTPAILTEAGQTYSGTEVRGDEKMIQDYY
                     GSRGYADARVETRLSDAGPGRLNVTFSVYEGEKSFIRKINISGNTKTQDRVIRRELPL
                     TPGDELNTVLLETSQRRLQNLNYFEGPGEANPLTVRPVSTEVPGFKDIEVNVTEKPTG
                     TINFGAGFSSIDSIVGFIDVTQTNFDISDWGDFRGAGQRFNMNIRFGPQRQDFNLNFT
                     EPWFMGQKLALSTELFYRNMFYLSDRFDQTNAGAAVSLRKPVGDHAYIEGMYTLQSIE
                     IDVDRRRSTTQVLRDEDGSFTQSKLDFAFVHDTRDDIFITRKGHKFETGLMVSGLGGD
                     AEVWGAHVGGIQYITIPGDGIVSFEGMLRHVDAWGSGDVPIFERQFLGGANNVRGFDF
                     REAGPKDVSGEPVGGSTSLFASVEYTFPIIEKVRGALFYDLAYITTDVEARNGVNAAG
                     VRNGGPIVGDGEIYSNIGIGVRMFLPIGPIRLDLGVPVQHDKFTGNSPKFQFNMGYKF
                     "
     gene            complement(27322..29328)
                     /locus_tag="PLP58_04505"
     CDS             complement(27322..29328)
                     /locus_tag="PLP58_04505"
                     /EC_number="3.1.6.-"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arylsulfatase"
                     /protein_id="HRJ07805.1"
                     /translation="MLRALFLACLVFATASLRSADQPNILLILTDDLGFSDLGCYGSE
                     IETPTLDRLAAGGIRFTQFYNTAKCHSSRVSLLSGRWCRQAGDESLARAVILPEVLQP
                     AGYFTAMSGKWHLSKQPTDFGFQRYFGHLSGACSYYKGDKTFRLNGQPWQVPETGFYT
                     TVAKVDHALGFLNEARAEKKPWFLYLAFNAPHAPLQPLEADYKKYLGRYDSGWDKMRA
                     ARLAKQRELGLFPPGTAPAPRPENIPAWESLKPEIRDWEARRMAAYAGMIDRVDQELG
                     RLVADLEAKGELENTLILFFSDNGACPYDRASTGMDAEPFDGVTSWSDSTGWARARNS
                     PFRYYKQNQFEGGIATPAILHWPAGIKAAPGSIVSTPAHLVDVLPTVADICGAQVPES
                     FPGREITPLAGISLKPVLEGGEIKPRPPIHFIFSSDRALRDGDWKLVSFQSEPWELYN
                     LAQDRTELHDVAAQHPDIVARMSAQWHEMTADVLRASPKERAPVAEKAGGHVHREWSI
                     YTGENGAVTSSRGMAGTTARKKSAATGKTLSIRARVGTKLRVEGGELILECSGDDPGL
                     AFDNLGALPSPGPYELSFRVQSRASGGGEVYFTTDAKTKLPAGRHVEFEVVHDGAWHE
                     VKLRLDTTETLHGLRLDPCAGPGSVRLGGLEMRDTTGKVLRAWP"
     gene            29465..32494
                     /locus_tag="PLP58_04510"
     CDS             29465..32494
                     /locus_tag="PLP58_04510"
                     /inference="COORDINATES: protein motif:HMM:TIGR02603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HRJ07806.1"
                     /translation="MKSSLRLLPLICAVSAATAQENDVAGNEQVRKIMETYTGRGTLA
                     DDTPPTEPLEAARKFKLRPDLAIDLMAAEPAVEQPLYASWDSQGRMWVTLYRQYQFPA
                     GLKIVSYDQHLRAKFDKVPPPPPRGPKGADKITVFEDTDGDGFFDKHTDVITGLNIAS
                     AALHGMGRIWVLNPPYLLSYPDADFDAMPDGDPQVELSGFGLEDTHSVATSLMWGMDG
                     WLYGANGSTTTGNVSSANTKNVKWEGQCIWRYHPRKKTFEIYAEGGGNTFSLDLDSMG
                     RVFSGTNNGKTRGMHYEQGSYGIKGWGKHGPLTNPYAFGWFEHMKHEGDEKRFPQAFV
                     IYEGGLLGSAYEGKIIAPNSLQNTVYVSERIPHGSTFRTKDEELLMSSPDRWFRPVWA
                     GVGPDGGFYMADWYDTRLSHVRPVDDWHKTSGRIYRVRPASGAPKLKPFDLSKASAEE
                     LFGYLSHPNRWFRRQAVLEIGWRGLNDLAPKLKDMLAGPQALEALWTLDGLDAVTDDA
                     LAGLLRHADPHVRRWAVKITGEKGREWTASTVMLLDRAGAEDHPEVRAQLLATAKRLP
                     ARAGLSMIWAANLPAPDDDTHLPLLAWWALESKAEKEREAVFTFLKSDTAFLQTELFK
                     NHLAEKLAKRYALAGGEANLNSCADLLALTDDAALRAKFIAGIASAFEGSELPPLPEA
                     LAKALNDYMAKQSGGDLTLALRSGSADALKKGLALLGNSKEPNAARIAVARTLSELGK
                     VEAVAPMVAILKSSNPASMKRGILQAASRFDDRRIAEALLTSYESQIAGDQALREDAL
                     RTLAGRKEWAVELIHFINEWKVPARHVPVDVVRQLSLHKDADIDAAIEKHWKGLLATG
                     PTEAKEKEMARIKAVLQTGLGDPEKGKLQYTVRCGICHKLFGDGNAIGPELTGYDRAN
                     ASFWLENIFNPSLEIREGFGNYIVKLKSGQLLTGIMDAQDASGIVLKDIAGNKTPVKQ
                     ADIETLEASPVSLMPEGLTTGMTEADLKDFFAYLMKP"
     gene            32536..33915
                     /locus_tag="PLP58_04515"
     CDS             32536..33915
                     /locus_tag="PLP58_04515"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HRJ07807.1"
                     /translation="MIARFTLSLLAAASLHAAPPNVVLIVSDDQGWPDLGCIGSKPVQ
                     TPNLDKLAERGVRGTSFYVTWPACTPSRGSLLTGRYPQRNGLYDMVRNDMVNYGHRYK
                     PEEYAVSPEMTLGLDPREITIGDMLRKAGYVNGVVGKWDMGQAKRYLPLQRGFDFFYG
                     HGNNGIDYYTHERYGTPSLFRGNQRTEEDKGKYATEVFRREALYFVNEHAGRNPFFLY
                     LPFNAPHGASTLAEDNGGQKPGVQAPEEYKARFRDIVKDEKLAGYYGAVTCMDDAIGA
                     VMDAVEKAGQTKNTIWIFMSDNGGSGNGGNAPLKGSKSTMWEGGLRVPFIFVWPGRAP
                     AGKVTDEFLTALEIVPTLLAATGAPAPEGVVMDGFDMLPVLRGEMKSPRGEMFWQRRG
                     DKAARIGQWKWLESAKGRGLYDLSTDLGESEDLSEEKPEVLEMMREKFAAWRKTMDEA
                     EPRGPFRDY"
     gene            33923..35116
                     /locus_tag="PLP58_04520"
     CDS             33923..35116
                     /locus_tag="PLP58_04520"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: protein motif:HMM:NF014081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008448 - N-acetylglucosamine-6-phosphate
                     deacetylase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0046349 - amino sugar biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HRJ07808.1"
                     /translation="MPPPLILTNATVILPGGLLENGAVLCRDGRIEWIGRVRKKWPAG
                     AEIADARGGFVSPGFVDIHVHGGAGADFMDGTPEAVRTVCKAHARHGTTTIFPTTTTG
                     APEQIQAMLAACAAVKKSWSPADGARIAGAHLYGPYFAEDKVGCHRVEGRRSPSPDEY
                     AAYFKGGLVRVATCAAELPGASSFYKAARRARCLVTCGHSNASWPEMAAAFKAGMRHV
                     DHFWCAMSSVPSVRARLGVPMRGSMAEFVLAEREMSTEVIADGCHLASELLEFAWRMK
                     GAGRLCLVTDANRALDMPPGRYRFGPEGDGSWFESDGRVGWAPNGSLASSIMGMDHMV
                     RVMRRDTTAPLHDIIRMAALTPAERAGIARECGSVEPGKRADLLVLNRRLQVRRVFIG
                     GTEVP"
     gene            35211..>37592
                     /locus_tag="PLP58_04525"
     CDS             35211..>37592
                     /locus_tag="PLP58_04525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009958504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dehydrogenase"
                     /protein_id="HRJ07809.1"
                     /translation="MRLIHLVSLLLLLAFPSPALLAQAAPKGKGKGGVKMKGDKSDPG
                     NWPDSSKGSAAPHAPQEEPPAATAARPQSATAPDSGERIVFLGNGLAERDVYFSRLET
                     EFHLRYPNEKLLIRNMGRPGDTPGFRPHPARASQWAFPGAEVFHPELDMHFGKGFFPT
                     PDQWLFHLKADTIVAFFGYNESFDGPAKVGNFEAELTAFVKHTLGKAYNGKAAPRLVL
                     VSPIAFENLSANRDLPNGEKENANLALYTAGIERVAKAHGLTFIDLFTPTKALYSKDR
                     SAFTINGFAPTEAGYQEIAVHLANGLYGHQSHQSKADPAKVHAAVKQKDWLWNNDYNL
                     VNGVHSHGQRYNPYGPQNYPDEVRKTREMMSLRDHLIHDLASGKLADISVDDSATHKL
                     PAVPTNFNPKDDKGKMGSIEYKNVEQALSSFTMAEGYKVDLFASESEFPDLKNPVQLS
                     FDNKGRLWVAVMPGYPHWKPGDPPPNDKLIILEDTDGDGRADKQTTFADGLQLPIGFE
                     IAPEGVYVSEEPNLILLVDENGDDKADRKELLLHGFDTHDTHHAISAYCADASGAFYM
                     GEGRFLHSQVETPYGPRRCNDGGVWRFDPKSFRLERYAQVDVSNPWGISFNDWEQCHI
                     SDGSPGESWWGLPVSAKMPYGIENPKVGQFVPKRSRPTSGAEFVSSRHFPDEHQGAFM
                     ICNSIGFLGTSMGKIRESGAGYEGELIADLLSSTDGNFRPVDIEFAPDGSLYVVDWHN
                     ALIGHMQHNARDPNRDHDHGRIYRITYPSRPLVEPAKVFGASIPELLENLKLPE"
BASE COUNT         6822 a        12357 c        12139 g         6274 t
ORIGIN      
        1 tgccgcatca ataaagtcaa caaagtcaat ccggtcaaaa aagcacccgc cccccgcagg
       61 ccgggcagtc tcacaccgcc acccggcccg cagccgcctc acacccccac caccttcgtc
      121 accaccgggc tgggcgccgc ctcccccgcc gggtttgccg ccaccacata ggcgtgcagc
      181 accgcgccgg gcgtcaggcc cttgatcgtg tggtcgaagt ccgtgtcccg gtccaggaag
      241 cggaagtcct cgtccacgcc ctccccttga cccgtgtggt gccaccccgc gccatgccgg
      301 gcgcttccgc acggcgcgcg cggcggtcag cgggcttttg cagtcagggt ggcaccggtg
      361 gatgcttttt tccgcttcct tgtctttttt gtttttcatt tcccgcgccg tagggttaat
      421 ttcccggctt taattcagaa ggttggcggt atttttccaa agcactccca tcaatccccc
      481 gccccgcccg ctcatgcacc cgcgcccagt ctcccagcca ggccgtcagc cggctccttt
      541 ccttcgctgc ctgcttttca ccctgccggc ctgcgccgtc ccccgtctcc tgcgcctttt
      601 gatcccgtcg tccaggctgc cttcatccct ccctttcatg aacacacgct cctccctcct
      661 gtgcttgcgc cgcgccctgc tcgcacccgc ccttggcctg ctgggcctgg gcggcaccct
      721 ggccgctgat gcacatgatg cgcccgccgc gcctgcggcc atccccttct cccagctcgg
      781 cgcggaggcg gacaagcagg gcgctcaggc ggcgagttcc atcacgccca cggccaccgg
      841 agcgcatctc aaggcgctga tgcaggacct ggaggccgag gccgcacccg acggcctctg
      901 gctcacctcc acggcggacg aggacgcggg caaaccgcac cgcttccgcg tgcgggcgct
      961 gagcctgggt agggcggatt gtcctcaatc cgccgcgagc gtctccccgg cggcgcttgg
     1021 aggaccaagc gccctacctc aatccggcgc cgtgcgtgct tcggcggagt ccgccctgtg
     1081 gctgcggccc ggcctggtgg aggaataccg cgtcagcacc gatggcgtgc gccaggactt
     1141 catcgtcatg cagcgcccgg cgggggcggg ggaggagctg gccgtggacc tggaggtcac
     1201 cggcgcgcgg gcggagacgg cggcttacgg cgtgaagctc atcgtcagcg gcacgggccg
     1261 ggagctggcc tacagccgcc tgaaggtcac cgatgcggcg ggcaaggaac tcaccgcgcg
     1321 cctcgaggtg gtggatgccg ggcgcctgcg cgtgctcgtg cgggacgcgg gcgcggccta
     1381 ccccgtgcgc attgacccca ccttctccga cgcggactgg gtggcgctca atccaagcat
     1441 ccctggggcg acctcctctg tgcaggtcat cacggtggat ggcagcggca atctgtatgc
     1501 gggcggggat ttcaccgcca tcgccacggt ggcggccaac cgcatcgcga aatggaacgg
     1561 cagcgcctgg agcgccttgg gcacgggcat ggacagcacc gttcgggcac tggtggtgag
     1621 cgggggcgat ctctacgccg ggggagactt cagcacggcg ggcgggatca gcgcgagccg
     1681 catcgcgaaa tgggacggca gcgcctggag cgcccttagc acgggcatgg acggcagcgt
     1741 ccaggcgctg gcggtgagcg ggggcgagct ctacgccggg ggaaacttca ccacggcggg
     1801 cgggaccagc gcgaactaca tcgcgaaatg ggacggcagc gcctggagcg ccctgggctt
     1861 gggcatgaac gccagcgtcc gtgcgctggc ggtgagcggg ggcgagatct acgccggggg
     1921 ccagttcacc atggcgggcg ggatcacggt gaaccgcatc gcgaaatggg acggcaccgt
     1981 ctggagcgcc ctgggtacgg gcatgaacaa cctcgtcctg gcgctggcgg tgagcggggg
     2041 cgatctctac gccgggggcg gcttcaccac ggcgggcggg gtaagtggca ggaaccgcat
     2101 cgcgaaatgg gacggcaccg cctggagcgc cctgggcacg ggcatgagca gcaccgtcca
     2161 ggcgctggcg gtcagcgggg gcgatctcta cgccgggggc ttcttcacca cggcgggcgg
     2221 gaccacggtg aaccgcatcg cgaaatggga cggcaccgcc tggagcgccc tgggcacggg
     2281 catgaacaac accgtcctgg cgctggcggt gagcgggggc gatctctacg ccgggggatc
     2341 cttcaccacg gcgggcggga cgggcatcgg ctatggaatc gcgaaatggg acggcagcgc
     2401 ctggagcgcc cttagcacgg gcatggacgg cagcgtccag gcactggcgg tgagcggggg
     2461 cgatctctac gccgggggag acttcaccac ggcgggcggg accagcgcga gctacatcgc
     2521 gaaatggaac ggcagcgcct ggagcgccct gggcacgggc atgaacggca acgtctatgc
     2581 gctggcggtg agcgggggcg atctctacgc cgggggactc ttcacctcgg cgggcgggac
     2641 cacggtgaac cgcatcgcca aatgggacgg cagcgcctgg agcgccctgg gcacgggcat
     2701 ggacagctac gtcaatgcgc tggcggtgag cgggggcgag atctacgccg ggggcggctt
     2761 caccacggcg ggcgggacca tggtgaaccg cgtcgccaaa tgggacggca ccgcctggag
     2821 cgccctgggc tcgggcgtaa gcgccaccgt caatgccctg gcggtgagcg ggggcgatct
     2881 ctacgccggg ggatccttca cctcggcggg cgggacgagc atcggctatg gaatcgcgaa
     2941 atgggacggc agcgcctgga gcgccctggg ctcgggcatg gacaacgtcg tccatgcgct
     3001 ggcggtgagc ggaggcgatc tctacgccgg aggagacttc agcacggcgg gcgggacggg
     3061 cgcgaacttc atcgcgaaat gggacggcag cgcctggagc gccctgggca cgggcatgaa
     3121 cacctccgtc cgtgcgctgg cggcgagcgg gggcgatctc tacgccgggg gagacttcat
     3181 catggcgggc gggaccagcg cggtccgcat cgcgaaatgg gacggcagcg cctggagcgc
     3241 cctgggctcg ggcgtgagca acgtcgtccg tgcgctggcg gcggacgcca gcgatcacct
     3301 ttttgtcggc ggcgcctttc tcaccgccgg cgccaccttc acgccctaca tcgcccaggc
     3361 gaacctgaat ggcggaagcg gagcccccga gatcgccctc agcggcaacg ggcaggacat
     3421 tgagaacggg gacgacaccc cggacacggc ggaccacacg gccttcggca gcgtggcggt
     3481 ggccggcggc agcgtggcgc gcaccttcac gatcaccaac agcggtgcgg cagtgctgaa
     3541 cctcaccggc acggctcctg actatgtgac cctcagcggc agcagcgctt tcaccgtgac
     3601 cacccagccc gctgcggcca ctgtcgcggc cagcggcggc acgcagacct ttgtcatcac
     3661 ctacgatccc tcggctgttg gcacgcacac ggccacggtg agcatcgcca atgctgatgg
     3721 cgacgagaac cccttcacct tcgacatcag cggcacgggc ttgaacagca cccccaccga
     3781 catcacgctg acgccggcca gcattgcgga agaaaacgcg ccgaacgcca cggtgggcgt
     3841 gctggacgcg gtggacgcgg acgtggcgga cacgcacacc ttcaccctcg ttgccggcac
     3901 gggcgacacg gacaacggca gcttcaccat cgcgggcacg gacttgaagc tcaccccggt
     3961 ggccgatttc gagacgaaaa acagctacag cctgcgggtg caggccgatg acggcgcggg
     4021 cggccttttt gagaaggcgc tgacggtgac gatcacgaat atttacgagc cagtcggcgg
     4081 cgcgcaggtc acgttcgtca atcccgccgg cggtgatttc agaatccagt ccatcaccgg
     4141 cctgaccgtc gcgggcaccg tgcccggcac gctctgggac ttcacttatt accacggggt
     4201 ggattacgcc agcgtgcccg gcccgcactt ggcggctgaa ggcctgattg atcacatcaa
     4261 tgcgggtcat gccgtcgtga acttctttgc cgccgccggc atagatgcca gcacgggcac
     4321 cgccttcacc gtgcgcggga acctgcccat cgccgcgccc tccggcggca atgtgaccgt
     4381 caacggcttc aaccgcagca gcagcgcccc gcacaatccg ctcacctacg gccaggcctg
     4441 ctgctacaac ccctccgaca ccgccgcgct gcccgcggac caggcctggg tcgtcatcac
     4501 gctccagccg gagccggaga tcgcggtgag cggcaacagc gtgaacatcg ccgatggcga
     4561 caccacgccg gacgcggcgg accatacgga cttcggcacc ctggcggtgg ccggcggcag
     4621 cgtggcgcgc accttcacga tcaccaacag cggcacggcg gtcttgaatc tcaccggcac
     4681 cgctcccgac tatgtgaccc tcagcggcgc gggcgccacc cacttcagcg tcaccaccca
     4741 gcccgcctcg gccacagtcg cggccagcgg cggcacgcag acctttgtca tcacctacga
     4801 tccctcggcg tccggcgcgc acacggccac ggtgagcatc gccaatgatg acggtgacaa
     4861 gaacccgttc aactttgaca tcagcggcac cggcaattcc gcccccacgg acatcacgct
     4921 cacgcccgcg agcatcgcgg aaaacaacgc ctccggcgcg gtggtcggcg agctggacgc
     4981 ggtggacgcg gatgcggcgg acacgcacac cttcacgctg gtggccggcg cgggcagcac
     5041 ggacaacgcc agctttgaga tagatggcga ggacctcaag ctcaacgcgg tggccgactt
     5101 cgagaccaag gccagctaca gcatccgcgt gcaggccgat gacggcgcgg gcggcctttt
     5161 tgagaaggcg ctgacggtgg cggtcacgaa cgagatcgag gcaccggcgg acctggaccc
     5221 gacttttgac acggatggca gggtggtggc ggacctgggt gggactgagg cggcgaactg
     5281 cgtggcgatc caggcggacg gcaagatcgt ggtggccggc acctccaacg ccagcggcag
     5341 cggcgacttc atggtcgcgc gctatctgcc cgacggcaca cccgacacca gctttggcac
     5401 gggcggcgtg gtgctgacgg atgtgagcgg cgcgggctcc gccgacctgg gacggcggct
     5461 tgcaatccag acggacggga agatcgtggt ggtgggcgag tcgaacgcgg gcaccggcgg
     5521 gctgaatttc gccctggcac gctacatgcc ggacggcaca ctggacagct gctttggcac
     5581 cggcggcctg gtggtgctgg atggcggtgg cggcatggat gacgggttga acgccgtcac
     5641 gctgcaggcg gacgggaaga ttgtggcggc gggctattct tggagttctt tcagcggcgg
     5701 cacgcgcacg gcggtggtgc tgagattcct ggccaccgga gcgctggacc cctccttcgg
     5761 tacgggcggc tcctggggcc tgaacttcgg cggctcgcag tactcggcgg gcggagtggc
     5821 ggtggatggc agcggcagga tcgtcgtggc gggcttcggc ccgcagccga actggaacgg
     5881 ctttgtcgcg cgcctcacgg ccgccggcag cacggacacc agcttcaacg gcggggtgca
     5941 gcaggccaat ttcggcgcct tcaccgaaag cggcttcaac gcggtgcggc tgcagagtga
     6001 cggcaggatc gtggtggcgg gcaaccgctc gaatgggtcc tatggtccca caacagactt
     6061 cgcgctctcc cgctatctgg agaatggaac gctggacacc agcttcggca ccgccggcat
     6121 gacgacgacg gaccgcgccg ggaggctgga caacatcatc tccatcacgc tcgacgtgca
     6181 tgaccgcatc atcgcggcgg gcatccagca ggacgcggcg ggggtggagg tcttcggcat
     6241 caaccgctac acgcgcaacg gcatcctgga caccaccttc ggcacgggcg gccgggtgct
     6301 gacgaccttt ggcgccgtca cggggaaccg cgcggcatgg acggcactcg acgccgacgg
     6361 acgcatcgtc gccgcgggct ttgcgggcaa tgacatcgcc ctggcgcgct atgagggcgg
     6421 ccccgcggcg cccgagatgg tcgtgaccgg caacagcacc ctcatcgccg acggcgacgc
     6481 gacgcccgcc acggcggacc acacggactt cggcagcgcg ctggcggccg gtgccacggt
     6541 ggcgcgcacc ttcaccatca ccaacattgg agacgccgaa ttgaacctca ccggcactcc
     6601 cctggtggaa gtgagtggca cgcacgcggc ggatttcagc gtgacactgg cccccgcgac
     6661 accggtggcc gaggaaggcg gcagcaccac cttccaagtg acctttgacc ccggcggact
     6721 cggcctgcgc accgccacga tcagcatcgc caacgacgac gaggatgaga acccctacaa
     6781 cttctccatc cagggcacgg gcttgaacag cacccccacc gacatcacgc tgacgcccgc
     6841 gagcatcgcg gaaaacaacg tctccggcgc ggtggtcggc gagctggacg cggtggacgc
     6901 ggatgcggcc gacacgcaca ccttcacgct ggtggccggc gcgggcagca cggacaacgc
     6961 cagctttgag atagatggcg aggacctcaa gctcaacgcg gtggccgatt tcgagaccaa
     7021 ggccagctac agcatccgcg tgcaggccag cgacggcaac ggcggccttt ttgagaagga
     7081 gctgacggtg gcggtgacga atgtcaacga ggcgcccagc ctggccagct cctatctggt
     7141 ttacgaaacc gtcacaccca cacgctccag caacaacatc gtgtattcgg tcaacaacgc
     7201 ggcagcgctg gcggggacgc ctttcacgcg ggtgcgctac cgcatggaga cgcgcgtcgg
     7261 cggcacgctg cgccatgccg atgtgagttt tgacaagtgg tccggcctga cagtcgccgg
     7321 actggcggtg ccgaccccca acaacccgtt catcgtccag cgcaatgtca ccaacatgaa
     7381 cgtggcctcg aactggtctg gccagggcaa tgccaccgcg gtcaccacgg ggtccggatt
     7441 caccggacgc cttgaaatct gggagcgaaa ctacggcacc gccaacgtat tcggcgatgg
     7501 caacggcagt ctctacgact tcgacgacac gccgtcagcg gggggctacg gctgttttca
     7561 ggtgcataat gtttcctcgc cgacgaagca caccgtgctc gcctggaaca accatggcag
     7621 taccactcca gacatcggct ttggcaatca gatcggtggc agcggccacc ccgactggac
     7681 cttcgcagga ggttccagcc tcggcaaaac cgactggaag ctgcagattt atgtgacgac
     7741 ggaacccgtg ttgtcgctcc cggagaactc cgccgcgggc accctcgcag gcaccctggc
     7801 cggcaccgat cctgacgcgg gcaacacgct cacctactcg ctggtttctg gcaccggtga
     7861 cacggacaac ggcagcttca cgatcagcgg caaccaggtg ctgagtgatg tggtcttcaa
     7921 ttatgagacg aagagcagct acaacatccg ggtccgtgtc acggacaatg gcgggctgtt
     7981 ctctgaatca gcgctgacgg tgacggtcac caacgtcaac gaggccgtgc tctcgctggc
     8041 gctgaccggg agcagtgtga acgaaaacgt gccggcgaac acgacggtgg gcaccttcag
     8101 cagcacggac accgacgcgg gcgacacgca cacctacacc ctggtcagcg gcacgggcag
     8161 cacggacaac ggcagtttca acatcagcgg cagcacgctg cgcgcgaacg cgggcctcaa
     8221 ttacgaggcc ggggccacgc gcagcatccg cgtgcgcagc accgacgcgg gcggcttgtt
     8281 cgcggaagac atcttcacca tcacgatcaa caacgtcaac gaggcgccga cgatcagcga
     8341 catcgccagt ctggcgatca acgaggacgc gaacaccggg gcgctggcct tcaccattgg
     8401 cgacccggac gcgggcgcgt ccctcaatgt gagcgccgcc tccggcaaca cgacgctggt
     8461 gcccttgtca agcatcgccc tgggcggctc tggaacggac cgcacggtga ctgtgacgcc
     8521 ggcggcggac ctgcacggca gcgccttgat caccgtgacg gtgagcgacg gcgctttgag
     8581 tgcccaggac accttcaccg tgacggtcaa cagcgtgaac gacgcgcctt ccttcaccat
     8641 ctccctgccc ggcggctcgg acctcgcggt ctccacgctg gcgggatcac ccggtctttc
     8701 aggcagcacg gatggcacgg gttcggcggc gcggtttaac aatccatggg gtggtgtctc
     8761 ggcggacgat gcgggcaatg tctatgtggc ggaccaggcc aatcacatca tccgcaagat
     8821 cacgcccggc ggagtggtga ccaccatcgc cggcagcgcc gggctgacgg gcagcgcgaa
     8881 tggcacgggc gcggcggcgc gcttcaacta cccgaacgcc gtggtggttg accccgcggg
     8941 cacggaactc tttgtggtgg accttgtgaa caacacgatc cgcagggtgg acctcgcgga
     9001 caattccgtg acgacctttg cagggacggc ggggttggcg ggcgcgtcca acgggactgg
     9061 agcggcggcg cgcttcaact acccgatcgg catcgcgcgc gactcggatg gcaacttgta
     9121 tgtgaccgag tcgaacaaca acacgctgcg caagatcacg ccgggagcgg tggtgacgac
     9181 cctggcgtcg ttcttcaaca acccctacca ccccgccgtg gacagcgcgg gcaatgtgta
     9241 tgtgccgcag cccaaccgcc acacgatcat caaagtgact ccggccggtg tgaagagcgt
     9301 cttcgcgggc acggacggca gttccggcgc cgccgacggc accggcgcgg cggcgaggtt
     9361 caacatcccc acaggcgtgg cagtggactc cgatgacaat gtgtatgtgg cggattacaa
     9421 caaccacacg ctgcgcatga tcaccccggc ggcggaggtg acaacggtgg ccggagtcgc
     9481 gggggccgca ggcagcacga atggcgcggc cagtgtggcg cgcttcaaca agccgctgag
     9541 cctgtcgttt gacggcgcgg gtgatcttta cctggcggac tcggccagct tcaccgtgcg
     9601 caagctgagc gctgatctgg ccccgctggt cgtggcggag gattccgggg cctactcggg
     9661 cgcggctttc ctcaccgcca tctcgccggg cccggcgaat gagtccgcgc agacggtgtc
     9721 cttcacggtg acaaacaaca acaacggact gttcagcgcg cagccggcca tcgccccgaa
     9781 cggcacgctc accttcacac cggcgccgga ccagaacggc caggccacgg tgacagtcac
     9841 cgctgtggac gacggcggca cggccaacgg cggcgtcaac accagcgcgc cgcagacttt
     9901 tgtcatcacc gtgacccctg aaaacgacgc gccagtcatc accagtgatg gcggcgggga
     9961 tgacgcgtcg ctcaatgtgg cggaaaactc cacggcggtc accaccgtca gcgccgcgga
    10021 catagacctg ccagcgcaga cgctgacctt caatatcacc ggcggggatg actccgccct
    10081 cttcagcatc ggcagcgcca ccggcgtgct ttccttcctc aacgcgccga actacgagtc
    10141 gcccgccgac gcggacctgg acaatgtgta tgaattgatc gtcgaagtct ccgacggcaa
    10201 cggcggcacg gacgagcagg agatcagcgt cacggtgaca aacgtcaacg aggcgccctt
    10261 catcacctat ggcggcacca cgccgccgtc cgtctggaac atgccggaga accaggtgga
    10321 cctcatcccc ttcgaggcgg acgaccccga ttttgagact gtgacgctga ccttcagcat
    10381 ccacggcggc gctgaccagg cgcgctttgg cattgacgcg gaaaccgggg aactggcctt
    10441 cctggcgacg cccgactttg agaaccccgc cgacgcggac accgacaatg tttatcacgt
    10501 cgtcatcgcc gtcaccgacg acggcacgcc cgcgctgacc accacccacg cggtgagcat
    10561 caccatcacc gacaccaacg acgcgcccgt catcatcagc aacggcggcg gcgcgctggc
    10621 cgcggtcaac gtgccggaaa acaccactgc ggtaaccacc gtcgtcgcca cggatgacga
    10681 cctgcccgcg cagacgctca ccttcagcat catcggcggc gcggacgcgg cgcgcttcac
    10741 cctcggcggc ggcacgggcg tgctggcctt caactccggc aaggaccacg aattccccag
    10801 cgaccagaac ggcgacaatg tctatgaagt ggtggtgcgg gtggaggacg acgcctcgcc
    10861 gcccagctcg gattcccaga cgatcctggt cacgatcacc cccgtggacg aggccccgct
    10921 ggcctggact catggtaatg aagcgccgac caccagcagc gcgggcgtct ttggcgaagc
    10981 cctgcccaac ggcctggaca ctagcgcatg gttcgagtac tccaccgacc cggccgtcga
    11041 gaccggcgtg ctgaccaccg ccgcgcagac gctcggcagc gcctcggtcc tggtggatgt
    11101 caccggcacg ctcaccggcc tgacggaggg caccacctat tacttccgcc tcgtggcgca
    11161 gaactccctc ggcatggtca aaggggagat cctcagcctg cacacgcagt cgctcttcaa
    11221 cggcaccgcc ccgctggcgg gcggcatcac aatcaagcgc ccctatccgg gaccaatcaa
    11281 cgaggccggc caggtcgtct tcaaagccca ggcgacgatc aagtcgggcc tcgtcagcag
    11341 cgccaacgac atgctgctcg tcaccaacag cagcggcacg ctcggcatca tcgcccgcga
    11401 aggcaccgtc atcaccggtg gcgcgcgctt caccggcctc ttcaaccatc cgctcgtcac
    11461 ggagggcggc caggccctgg ctctggagaa gctcgccaac gccaccactg tcaatgacta
    11521 cctctatccc gtctcagagg acggcgtcag cctcgaccaa ctcagccgcg agggcgacag
    11581 cgcccctgac tcggacggcg gcgtcttcct cggccacgcc aaccggcccg ccgccgacgg
    11641 ccaggaccgc gtgtatttct cctccagcct caccggcgtc agcaccaaaa aggacagcgg
    11701 catctggtat gacgatggcg gcgtgctcac caagctcgtg cttgaaggcg agaaggcccc
    11761 cgcgggcgat accaccgcac tccaggcatg gtacggcaac atccagagca tggtggccgc
    11821 cggcggcgac ggcgtggcct tcatcgccgc gctccagggc aatcccggtg acaccaccca
    11881 gaagaccagc accgcgatga acgcgggagt cttcgccggc ccgccgggag acatcgccat
    11941 catcgcgcgc aaaggcggag tcatcaccgg cacgggcacc ctcaacgcct tcaacgccgt
    12001 ctcccgctcc tccaccggcg gccacgccta cctcgccacg ctcaagaaca gcagcgtcgc
    12061 gccagtggtc accacggcca acaaccaggt gctcgtcatc aagacgggca cgctggaaaa
    12121 catcgtcgcg cgcaaaggcg tcaccgtcat caccggcgcg ctcacgccct cgaagttcgg
    12181 caacttcacc atcaccgatg acgacgaagt ggtcttccag gtcaccctgg ccggcaccgg
    12241 agtcaccacc gcgaacgacc aggccctgtg ccgctggacc gaggccggcg gcatcgagct
    12301 gctggcgcgc gagggcagcg ccgcgcccgg catcggagcg aattacctgg ccttcctggc
    12361 cttctccgtc agcccctccg gcaacatcgt cctgcacagc accctcagcg gcaccggcgc
    12421 cagcgccctg ctgcgcgaca ccggctccgg cctggcgctg ctcgtgcgca atggcagcct
    12481 cgtcccccac ggcaccaccc agcgcgccgt caaggccatg agcatccaca ccggctacgg
    12541 cgacggcggc ggcgcgggcg gcatgggcgc ggccatcaac gacaatggcg aggtcttcac
    12601 cgtgctagac cttagcggcg tctatcaggc caaagtgttt ccgtgacgcg gacggtcttg
    12661 cgctccgccc tctttatctt ttcgccctct cctcaaaccc ggcgccgcct tcgccgcgct
    12721 gccagtggag aggggcggcg ccgctccact ccttgaagac acgggagaag tgggcggcgc
    12781 ttttgtagcc gagctcccag gcgacggctt tcacctgggc gtcaggctgg cggagcttga
    12841 tggcggcctg ggagagcttc agacgggtga ggcaggacag gggtgtctcg ctgtcgtagc
    12901 gcttgaagag gcgggtcatg taggcggcgg agacatggca gtggcgggcg gcctcctcga
    12961 tgctgccgag cacgggatag ttccgcagca ggtggccgcg gcagcgcagg taggtggcgt
    13021 aggcggggtc gagcttggtg gcggctggct gccggctgtc tgcgcagagc acgagggcat
    13081 gctcgagagt ggcagcggcg gcgcgcaggc cgagacggcc tccacgcaaa gcatggtcaa
    13141 tcacctcctc cagcaatgcc gtgacacggc ccgcgtcaag cacccgaatg actgcgcctg
    13201 gaccaagacc caactcttcc agaagggcgg cggcacgaac gccggcaaag ttgaaaaaat
    13261 acttcaccag cgggtcctcg ctgccggagc ggatcagatg cggggtgccc gggtcaaaaa
    13321 aatacgcatg gccggtgccg atctcctgct tcctgcccgc aagcgtcaca tccccttttc
    13381 cgcgccccac aaactcaaag gcgtgaaaag gcagtgactg gcggtccacg acaaaatccg
    13441 gcgcgcacca ctcgcagccg ccgccgacaa ggcacaggcc aagcttttcc cgctggcgcc
    13501 gccgccagtc aggcaggtaa aagcgccggg tgcgcagcac ctgggtggaa aagtagtcgg
    13561 cttgttcagt ggctggcatg gtcggcctgg agggaaaacg gcgtcacagg ctcatcttgc
    13621 gcggcgggtc aagaatcgtc aaccggctgc caaggatggg cacaggcgga ttcttgcgcc
    13681 cgcaaaggtg gaacgtttca tgcgcccatg caaccacccc agcccctgcg cggcatcatc
    13741 ccgcccctcg tcaccccgct gcgcgaccgc gacacgctcg acacggccgg cctggagcgg
    13801 ctcgtcgagc atctcatcag cggcggtgtg agcggtcttt tcatcctggg taccacgggt
    13861 gagggaccga gcctgagcta ccggctgcgc cgtgaactgg tccagctggc ctgcaagttc
    13921 gcgaacaacc gtgtgcccgt gctcaccggc atcacggaca cctccttcac cgaaagcgtg
    13981 aacctggccc gccacgcggc ggactgcggc gccacgcatg tggtgacttc ggccccgtat
    14041 tacttccccg ccgctccgcc ggagatgctg gagtatgtgc aggacctggt ggccgagatg
    14101 ccgctgccgc ttttcctcta caacatgccg ggtctgacga aagtgagctt tgaactggac
    14161 ctggtgaggc gggcgctgga tatgcccggc atctgcggag tgaaggacag ctcgtgcgac
    14221 atgatctact ttcaccgcct catcgaggtg gcgcggcagc gcgcggactg gagcgtgctc
    14281 gtcggccccg aggagctgac cgcggaggcg gtgctgcttg gcgggcacgg cggcatcaac
    14341 ggcggcgcga acctgcatcc gcggctctat gtggatatgt accatgccgc cgccgcgcaa
    14401 gacctccagc gcacgcgcga gttgcacgcg caggtgatga gcctggcggg cgccatttac
    14461 accgtgggcc ggcacaagag cgccatcatc aagggcatca aatgcgccct ctccctcacg
    14521 ggcgtctgcg aggaccacat ggcggagcca ttccaccgct tccgcgaacc ggagcgcggg
    14581 ctgatgcggg agcgcctgat ggccatcggt ctgctggctt gaaaacgcgc cggccatccg
    14641 gcatggcgga gctcaccagc ttttctcaag cgtcatcacg cactccaggt gccgcgtctg
    14701 cggaaagagg tcgaaaggct ggactttgag cagattgaac ccggcttcgg tgaacagggc
    14761 gagatcgcgc atctgggtgg cggggttgca ggagacatag acgacacggc gcggggcgaa
    14821 agcaaagagc tggcggagga aatcctcgct gcacccggcg cgcggcgggt ctatgatgac
    14881 cacggtttcc gagccaccgc cctcaatgga ttcgaaaatg gcggccgcgt cggcggcgag
    14941 aaagcggcag tgggtgaggc cgttgatctc cgcgttgtga gcggcttttc tcacggcgcc
    15001 ttcggagatc tccacaccga tgacctcctg aaactcacgc gctgcgctga tggcaaagag
    15061 gccgctgccg cagtaggcat ccacaaggcg gcgcgctccg ctggccgcgg cctcccgcac
    15121 ggcgtgggag acgaactccg gcaggatgaa ggggttgttt tggaaaaaat cgcccgcatg
    15181 aaactcgaag cggaccccgc caacgtcttc aacagcggtc tcatccgggc gcgtcagcac
    15241 tccgttggcg gcggcgcgca tgagcagggt ggcgccgttt ttgaaggtgc ctggcacagc
    15301 gcgcgcggcc tcgcgcgcgc ggctgagttc ggcattcaag gcatccatgg cgatggggca
    15361 gcgcgccaca tccaccatgg cattgcgcgt gcctgcgcgc aggaagccga tggcgccgat
    15421 ttcgccattt ttcggctttt gaaaatgcgg ggtgatcttt gaccggtagc cgtattgacg
    15481 cggcgaggga atgactggca ggacgggatg cttaatgcgc gccatgtgcc agagcagttc
    15541 cccgacctgg cttcgcttcc attcaagctg cctgtcataa gccagatgct gatactggca
    15601 gccgccgcac tggccaaaga gcgcgcaccg aggctcgaca cggtcgggcg agggttcgat
    15661 gacttcgacg aggtcggcct cggagtagtt cttgtggttg cggaacacgc gcgcgcggac
    15721 acgctcgcct ggaagcgcga aggagacaaa gaccacccag ccgtccaccc gcgccacgcc
    15781 gcgcccctcg ttggcgaggt tttcaatgac caggtccagt tcttgatgat agctgaaagg
    15841 gtggggctgg aatttgcgcg gaggcatggg gattgcggat aggtgaatgc tttctggctg
    15901 aatccttgct tgaggacaag cgggtatgca ctgaagaaat gccatgctgg acacgcgcaa
    15961 tgaaattcac cgtttgatga ggcggttcga gttcgactct ggccgggcgg tgctgaagtt
    16021 cagtctctgc gcgctggctg cctatgcggc ggcggcatgg ccggagcatc ccgggctggg
    16081 ggacgcgggg cgctgctcgc tgggcatcgt gatgctgggg gcgggacttt ggatcactga
    16141 ggcgatcccc gcgttcgccg tggcgctgct ggtgatcgga ctgcaaatta tcaccctggg
    16201 acgcgagggc ggcgtgctgg cggagacggg cgactcgaag gcatgggagg actatgtgag
    16261 gccgtggtcg agtccgccga tgtggctttt ctttggcggc ctggtgctgg cgcgcgcggc
    16321 ggagcgcacg ggcctggacc ggcatgtggc gggcagggtg ctggcgtgga cgcggggcag
    16381 cccgtcacgg ctgctgccgg cggtcatggc gctggctttt gtgttcagca tgttcatgtc
    16441 gaacactgcc acggcggcca tgatgctggc gatgctgcgc ccggcgctgg caagcctgcc
    16501 tgaggggtcg aagacggcgc gcgcgctgct gctaggactg gcctgcgcgg caaacctggg
    16561 cggcatggca accatcatcg gcactccgcc gaacgccatc gccgcggcgc ttctggagga
    16621 cgatgctcct gtggattttc tacgctggat gtttctggcg ctgccacccg cgctgctgct
    16681 cttcgccgtc gtgtgggccc tgctggcgcg gcctctgatc cgtgaaaaat ccgtgctgcc
    16741 gccgctgcag gaggcgccaa gggagggttc gggagtccgc cgctggcaga gactgctgac
    16801 gctggccgtg tttgcagtga cggtgctgct gtggatgtcc ggggagtggc acggcattcc
    16861 gaccggtgtg gtggcttttg tgcccatcgt ggcgctgtcc atggccgggg tgatccgcaa
    16921 gcaggacatg cgcgccattg actgggatgt gctcatcctg ctggcaggcg ggctttccct
    16981 cggtgtgggg attgaaaagt cggggctggc cgagtggctg gcggggctgg tgcagcaggc
    17041 ggaaatgccg tcctggcagg cctccttggt gctctgcctg atcgcggggg gcatttcgaa
    17101 tttgatgagc aacacggctg ccgcgaacat cctcctgccg gtgacactcg tcatcgccca
    17161 ggccatgggc ggagacgcgg atgtgaccat gttcgtcgtg ccggtggcgc tggcctgctc
    17221 aacggcgatg gcgctgccga tctcgactcc gccaaacgcc atcgtctatg ccagcggacg
    17281 cctgcgcggc accgactacc tggcgcccgg cctcctgacc ctcgtgcttg ggcctgtcct
    17341 cgcgctcggc tggtgcatga tcgccggatg agcgcgccta atccacatgc acgaactcgt
    17401 tcgagttgat caaggccagg cagtagtcgg ccacggcatc ctggaaggcg ggagcgaggg
    17461 cggtctgccc gtccagaaac tgccgcgccg cgtcccgctc agccgctgac gccgcgcggg
    17521 aaaaacagcg ccggaccgcg aaatcagcga ttgcgcccat gtccgaaccg caggcggcca
    17581 aagcctgccc ggcgagggtg gaggcaatgc cctgggtgag ctcggaattc atcagcagca
    17641 gggcctgcgg agccgtggtc gaagtctcgc ggcgcgagca cgtcaccagc gcatcgggcc
    17701 ggtcaaagag gtcaaagatg gggtgccgcg cgttgcggcg cgcgaaggcg tagatgctgc
    17761 ggcggcgcag ctcgctctca tccacggtgg cctcagcctg ctttttcaaa agcgtgccgc
    17821 tgatctccgc aggcagaggc aggcgcgcac cgggaccgcc ggccctggcg ttgagttgtc
    17881 ctgtgatcac gagcaagctg tcgcgcagca tctcgccggt cagacgcttg cgcggcatgg
    17941 cggcataaag gccgatggct tccccgctca tgccgggcgg aggcgtggcc tgacggaagg
    18001 tttgggagag aacgatggcc ttgtgcaggc gcttcaagct ccagccctgg cggggcagtt
    18061 cggcagccag ccagtcgaga agctcgggat gcgtgggcgg cgggctttgc ctgcccagat
    18121 cggcgggtgt cggcgcgatg gcgcggccaa agtgctgctg ccagactcgg ttggccgtgg
    18181 cccgcaaaaa aagcgcgttg tccgcgcgcg tgatccatcg ggccagcgcg gcgcggtccc
    18241 cggcaggctg gtccagacca ccggcgatgc gcggatagcc gggctggatc accgggccgg
    18301 gccggcggaa ctcgccgcgg acgaagaccg tgctggcggg cactccctcg gtgaagacac
    18361 gcacctgcgg accgagcggc ttgtctctcg cggtgaggac ggtgttgtca aaaaacgcgc
    18421 gcaaccggta aaagtcggcc tggctgagag gatcgtaggc atgatcgtgg cactgggcgc
    18481 agccgacggt gaggccgagg aagaccgacc ccgtggtgct ggtgatgtca ttgagcacga
    18541 catggcggcg ctcgtcctga ttgttgaggt cgggcatgtc cggaccggag aaaaggaagc
    18601 ccgtggcggc cggctctcct ccgtccagaa ggtcgccggc gatttgctgg cgcacgaatt
    18661 catcaaaagg catgtcttca ttcagcgcgc ggatcaccca gtcgcggtat tgccatgcgc
    18721 gcggcctttc cttgtcatgc tcaaagccgt cggtctcggc gaagcgcgca agatcgagcc
    18781 accactgcgc ccaatgctcg ccataagccg gactggcaag caggcgctcc accagagcgt
    18841 ggatggccgt gtctttgtcc cgggcgcacg tcttgatgaa ggcatccact tcctcgggag
    18901 tcggcggcaa acccgtgaga tcaaaggaca gacgccggat gagcgtggcg gcatcggctt
    18961 cgtcggagcg ggtcagaccg gcctccagca gacgcttttc aatcaagccg tcgagttccg
    19021 cggacgcgcc ccgtgcctgc ccgctgcgcg cgggtttgac ggggaaagcc aggggctgga
    19081 aggaccagtg cccgcgctca tcctccgtga tcggaggctc gtcccgcacc gggtcggcgg
    19141 aggcgcgaat ggctctggca tggaccgcgt catgcaggac aagggaggcc aggatgacag
    19201 ggagcgcttt catgattcag gccaggatgc cgcgcaccac attgccggcg acatcggtaa
    19261 ggcgctcgtc acggccgttg tgacgatggg tgaggcgctc gtggtccact ccgagcaggt
    19321 gcaggatggt ggcgtgcagg tcatgcacat gcaccttgtc cacctccgcg cgcaggccga
    19381 agtcgtccgt ggcaccgtgg acatggccgc gcttcacacc cgcgccgaaa agcaccgtgg
    19441 tgtaacccca gggattatga tcacggcccg tgcccttttc actcatcggc atccggccga
    19501 attcgccgcc ccagatgaca agggtgtcat cgagcaggcc gcgctgcttg agatcgagaa
    19561 tgagcgccgc gacgggctgg tcggtgcgcg ctgagtagtc agtgtggttt ttcagcacat
    19621 cctcatgggc atcccagcca ttcgtgtcac cggaataaag ctgcacaaac cgcacgccgc
    19681 gctccacgag ccggcgcgcc agcaggcagc gcgtgccgaa ctcgcgcgtc accggctggt
    19741 ccaggccgta gagagttttc gtcgcgtccg actccttcga aaggtccacc gcctccggcg
    19801 cggcgctttg catgcggaag gccagctcgt aggcgcgcat ccgggcatcg agatgcgtgt
    19861 cctccgcgcg cgcggactgg tgcgcttggt tcagccggtt cacgaggtcc agcgttgagc
    19921 gctggcgcgc gctgctttgc cccccggcgg ggcgcagatg caggatggga ttttcccccg
    19981 gcctgagcgt ggtgccctgg tgggtggcgg ggaggaaggc gctgccccag gcaggcggtc
    20041 cgcctttcag cccaccgccg gggtccggca tcacgacaaa ggcgggcatg tccgcgttct
    20101 cggagccgag gccgtaggac acccagcttc ccaggctcgg cttgcccatc aagatcgagc
    20161 cggtgttcat ctgataaacc gattgcggat ggttcacgct gtcgccatga caactgcgga
    20221 tgacgcacat ctcatccgcc agctccgcca tgcgcggcag gaacccgctg atctcgatgc
    20281 cgccggcccc gtgccttcga aacggcttca cggggcctaa caagggattc tccgccacct
    20341 tccgccgcgt ctcgaccgcg cccaggctct ccggcagggg ctttcccgcg tggcggacca
    20401 gctccggctt gggatcaaaa agatccacat gactcgggcc gccatgcatg aacagccaga
    20461 tgacccgctt tgccttcgcc ggaaaatgcg ccgcccccgc cggatcatgc tcagcggcca
    20521 gcagatggga aaatgccacg ccgggcagga cagccgccgc tccgccaaga agacggcggc
    20581 gggagacgaa cggaggcgtg gccggagaca tgacactcaa acgcgcgagg tcgcggcctt
    20641 ttagcctgta ttgaggcatg gagcactcgc gcgcctgcga caagcggccg tccgcttcaa
    20701 cggtcatgga tggttcattt tgtttcttgc gccagggctg gtccgcgggt catatccacg
    20761 catggcttcc atcaccaccc aggaattgtc ggatcacgcg ctctggctcg aatcttcagg
    20821 cgcgcaaggc aggcgtattg acgagactgg tctgaacctt tccggccacg atctggcagg
    20881 actgaacctc gccagggcgc agtttgtcgg aacagtcttc gatggcgcgg atttggcggg
    20941 gacgaatctt tccggcgcgg acttcagcgg agcgaccttc gaaggcgcga atctcacggg
    21001 tgctgatctt ggcggagcgg acctgggcgg ggtcacgctc agcggcgcga acttgtccgg
    21061 ggcgaatttg cgcggagcgg acctctccgg cgcgcggctg gacggggctg acctgacggg
    21121 ggcggacttc acagacgcgg acctcatcgc ggcggatctc acaggcgcga agcaaggcgg
    21181 aatgatcctg gccggcgcca agctcaacga cactcgcggc ctggagtgag cggaccgctc
    21241 acctctccgc cgggcgcccg gcggccagac tgccgccgag ccatcggatg agttcctcga
    21301 cactggcgtg cttctccctg agggaggaaa gtgccgtcca atccattcgc tggcgcaact
    21361 gcggcgctgt cacacgctgg gggcgcagct tggcgagcac gctgtcgaac tctgcggcac
    21421 tcatcctcgt gatgtcggcg gcggagggca gttcgtcgag ctgggcggtg ttgatgccaa
    21481 ggctgctgat gccaaccccg aactggctgc ccagggagcg cagcgccctg acgagggctt
    21541 cgtcgttgag cggctccgcc acgaccatct cgctctggag tgtccagctt gtggcgctga
    21601 gggtctgaaa aaactcggcg cggcagttct ccagggtgac gccgcttttg atctgcacgc
    21661 ctgtgatgcc ggcttcggga ccaccgagat gccggcgcag attgatcatg gcggagtcga
    21721 aacgcgtgac attgtcgtcc gccccggttt ccagatccca ttcggcaatg acgaggtcag
    21781 ggatgtccca atgcccccgc acttccagtt cctcgcggcc ccgtccattc aactgaaagg
    21841 ggtgctgcgc caacggcagg ctccgccgct catagatggc cagcaggcgc aaaaaacgcg
    21901 ccactctcgc atccccctcc ggccccgccg caaaggacag atacgcctcc gatgtggagt
    21961 ccggcgcgcg cctggcctgg cgcaggtagt atccctggcc ctgatccacc ttggcaatag
    22021 gggaggtgga gtcgtaggac agaatggaga agtggtagcg cagcgtggcg tcactgtaat
    22081 ccccggccag ccgcagccgc accatgcgga tgagctcggt gcccttgatg gcctggtcgg
    22141 gatcgtcggg caggatctcc gggaggagga ttcgaagttg ttcgcgcagg ttcattccgc
    22201 gtgctgggcg ttcatttcat ccagcatgaa tgcggcggac cgcgcgggtc aagatggaaa
    22261 ggatgaatct ttgcgccgcg cgaccacacg cctgctgttc aaccagcgca cgccgaacat
    22321 gtccatggtt gcgcgccagg cccggttgcc gaagccgtat ttgctggcgc catgcaggcg
    22381 cgggcggtga ctcacgggca tctcagtgac acgccagccc atggcggcga cgagcgcggg
    22441 gatgaagcgg tgcattccgt tgaagggaag cagcgcctcg cggcactccc tgcgcatgac
    22501 tttgagcgtg catcccgtgt catgcacatg gtcccggacg aaacgggagc gcacggtgtt
    22561 ggccacgcgg ctttgcagcc gcttgaacat agagtcctgg cggctcacgc gccatccgca
    22621 gacaagatcc cagccttcat ccagccggtt tagcattgcg ggaatgtcag ccggggcgtt
    22681 ctgaagatcg ccgtcaagca tggcaatgac ggccccgcgc gcatcatgaa tgcccgcgtg
    22741 catggcggcg ctctggccgg cgttcctctc aaattcgacg agccgcgtcc gcgcgtccgg
    22801 acggacgcgc gccactgtgc cgtcggtgct gccgtcatcc acgagcacca cctcgtaatc
    22861 cagtccccgg agcgcggcgg cgatctcctc ctggagccgt gccacattgt cctgctcgtt
    22921 gtaaagcggc acgacgacgg aaagggcggg cgcgctgctc atgacgaggc atccttcgcg
    22981 gcggactcca tctcggaaag cctgcgctcc agctcgcgca ggcgcgcgag catgtctgga
    23041 atctttgccg gaagcgccat cagctttcgt ccctcgatca acggtcttgc cggaaatcct
    23101 gtgtaaacgc cgggttcagt gatgcttttg gtgacgccgg acttggcgag aaccgtgaca
    23161 ccggagccta tctcaagatg accggcgact ccgacctggc ccgcgagcgt gacacagtca
    23221 ccgacatgcg tgctgccggc gatgccgacc tgggagacga tgacacagtg tttcccgacg
    23281 accacgttgt gggcgatctg caccaggttg tcaattttcg tgccctctcc aatccaggtg
    23341 aggccgaagc gcgcgcggtc tatcgtcgcg caggacccga cctcgacatc gtcgtccaac
    23401 cgcacgatgc ccacctgctc cactttgaca ttgcggccct gggtgaactc gtagccgaaa
    23461 ccgtccgagc cgatgaccgc tcccgcgtga atgatgacgc gcgcgccaag aacacagcgc
    23521 tccctgaccg tggaattcgg atgcagcacg catgacggac cgagactggc gcccttgcca
    23581 acgaacgcgc ccgcgtggac gacgcagcca tcgcccaggg aggccccctc ctccacgatg
    23641 gcgcaggcac cgatgtggac ttttcccgcg tcgaacgaga cgcccggcgc gacgaccgcc
    23701 gaggcatgca ctccgggttt cgcaggagcc gccgccggga tgaaatggcg gatgacagtg
    23761 gagaatgcca gagtggggtt ctccacagcg atcaaggccg ccatgccgca agccagcctg
    23821 gccgacctgg cgaccagcac tgcggaggcg cgggtggtgg cgagcgcctc cgcataacgg
    23881 tcgttgccga gaaaggtgac ctcgccgggc tgcgcctcaa cgatggagtt gaggccgctg
    23941 atcggtgtcc ggggatcacc acggaggatc tcgccaccga ccagctccgc cagcttgttg
    24001 aggggaatgc tagaactcat gctcgtatgg aataaaaaaa ggcacgccga cgtgcaacgg
    24061 cgtgcctttc gggaaagctt gtcgtcacgg cttgccggcg gctgctccgg cgggagcatc
    24121 ggctgcggga gcggcatccc gcttcttgtt caactcgaca atgacctggt cggtaatgtc
    24181 cgccgcgtcc ttgtaataga gcagaacagg aactgtggac aggctcgcgc cggttttgtc
    24241 gaacacaaaa tcgaatccct gcgccttggc atgatccctg accacggcga cgatctcagc
    24301 gtagaggtcg cggcggattt ccacgtcctc ctgcttgagc tggttggtcc gccggttctg
    24361 cgcctcggtg atctcctgct ccagcgcgcg cgcctccctt gccttttcct cgaactcgcc
    24421 cgcctttttg ttgcggacat ccggcgagct gatggggtcg ctgatttcct ttttcagctt
    24481 ttgcatggtg tccatgaggc ccttgtaaac ggcccaccga tcgttcatct ccttctgcgc
    24541 tttttcaaca ttgcccttga atttgtccgc cgcttcctgg gttttttgaa attcggaaaa
    24601 cgccttggac atgtcaacca cggcaaattt gagatccgcc gccggagcgg acgtcagaaa
    24661 ggcgctcgtg agaatgagga tcgaaaaacg aagcatactt gtggtggggg tgggctgaag
    24721 gaggggttgg atcaaaattt atagcccatg ttgaactgaa atttcgggct gttgccggtg
    24781 aacttgtcgt gctggacggg aacgccaaga tcaaggcgga tcgggcctat gggcaggaac
    24841 atgcggacgc cgatgccaat gttggagtaa atttcaccgt cgccgacgat gggaccaccg
    24901 ttgcggacac cggccgcgtt gacgccgttt cgggcctcca catccgtggt gatgtaggcc
    24961 aggtcgtaaa agagcgcgcc acgcactttc tcaatgatcg ggaaggtgta ttcaacgctg
    25021 gcaaacaggg atgtgctgcc gcccaccggc tcgccggaga catccttggg acccgcctcg
    25081 cggaagtcga agccgcgcac gttgttggcg ccgccgagga actggcgctc aaaaatcggc
    25141 acatccccgg aaccccaggc atccacatgg cggagcatgc cttcgaagct cacaatgccg
    25201 tcgcctggga tggtgatgta ttggatgccc ccgacgtgtg ctccccacac ttccgcatcg
    25261 ccgcctagtc ctgaaaccat gagaccggtc tcgaacttgt ggcctttgcg ggtgataaag
    25321 atgtcgtccc tggtgtcatg cacgaaagcg aagtccagct tgctctgggt gaacgaaccg
    25381 tcctcgtcac gcagaacctg cgtggtgctg cggcggcggt ccacgtcgat ctcgatgctt
    25441 tgcagggtgt acatgccctc aatgtaggca tggtcaccaa ccggcttgcg caggctgacg
    25501 gcggcaccgg cgttggtctg gtcaaagcgg tcggaaagat agaacatgtt gcggtaaaac
    25561 agctcggtgg agagggccag cttctgcccc atgaaccacg gttccgtgaa gttaagattg
    25621 aagtcctggc gctgcggacc gaagcggatg ttcatgttga agcgctggcc ggctccgcgg
    25681 aagtcgcccc agtcggagat gtcgaagttc gtctgcgtca cgtcaatgaa accgacgatg
    25741 ctgtcgatcg aactgaagcc cgcgccgaag ttgatcgtgc cggtgggctt ctcggtgacg
    25801 ttgacttcaa tgtccttgaa accgggaacc tccgtgctca ctggacgcac ggtgaggggg
    25861 ttcgcctctc ccgggccctc aaagtagttg aggttttgca acctgcgctg ggaggtttcg
    25921 agcaggacgg tgttcaactc atcgcccgga gtcaggggca gttccctgcg gatgacccgg
    25981 tcctgcgttt tggtgttgcc gctgatgttg attttgcgga tgaaggattt ttcaccctca
    26041 tacacgctga aggtgacatt caaacggcct ggaccggcat ccgacaggcg ggtttccacc
    26101 cgtgcatcgg cgtaaccgcg tgagccgtaa taatcctgga tcatcttttc atcgccccgc
    26161 acctcggtgc ctgagtaggt ctggccggcc tcggtgagga tggcgggggt gagttcatcc
    26221 ttggagaata gtgtcacgcc ctccaaggca acatccgcca cctcatactg cccgccctcg
    26281 ttgatctcaa agaccagcgc cacgcggtcg gaactgacga cctcccggcg ataccccact
    26341 ttcacataca catagccctg gtcctgatag agggtttcga ttttgcgcac atcctcaagc
    26401 acggcttggt tgtccagctt gccggccttg ccccacaagc gccagaaggt cttctccttg
    26461 gaagtcaaag ccgacctcag tttgcggtcg ccgatggcgg agttgccttc aaagcggatg
    26521 tcgccgatca ggcccttgcc gccctcctcg attttaaaga gcacgctggt gaagccgggc
    26581 ttggtcgaag gctgggtctc gtaggtgacg agggcgtcgg aaaagccttt cttcgtgtag
    26641 aggtccagga ttttctgctg cgcggcggcc agtttgatct catccaccgg atcacccacc
    26701 ttgacttcga tctccttgcg cagcttgtca ttgtcaaagg cggcgttgcc gacaaagcca
    26761 atctcgccaa tgcccccgcg cccgtaaatg gtcaccacga ccctgactcc gccggacaca
    26821 ttgacggcct ggatgtccac gttttccacc gcgccggttt gatagagatt gcgcaggtcg
    26881 cgctcgacgg cctcgtccga gtagggttcc ccgacccggg tggacatctg ggcgcgcacg
    26941 cggtcagggt ccaacgccgc cgcgccttgg aattgcacat ccacagcctg gacgattctg
    27001 ccgccgcgcg atgccgccgg agtgtccagg gaaacctggc cctgcgctgt cagggcagat
    27061 gtggtgatga gcacggccag ccagggacgc atgaggaggg aagcgcggga gaagactcct
    27121 gagatcatga taaaggaagt ggaacttggc tacgggagga tgatgtgctt ccaggctgga
    27181 agaaccgccg cagtaatgct gatcatcaag aggcgcgtca aggtctaaaa acgccattca
    27241 acagcgctgg cgtttgatcc tgccccgggc accctcatca agtccgcctt ctcgaagcgt
    27301 cagtgctcgc attttcttgg ttcacggcca ggcgcgcaag actttgcccg ttgtgtcccg
    27361 catttccagg ccgccgaggc gcacgctgcc cggaccggcg cagggatcga gccgcagacc
    27421 gtgcagtgtc tctgtggtgt cgaggcgcag cttgacctcg tgccatgctc cgtcgtgaac
    27481 cacctcaaac tccacatgcc tgcccgctgg cagcttggtc tttgcatccg tggtgaagta
    27541 aacctcgcca ccgccgctgg cccggctttg cacgcgaaag ctcagctcgt aggggccggg
    27601 cgagggcagc gctccgaggt tgtcaaaagc cagcccgggg tcatcgccgc tgcattcgag
    27661 gatcagctcg ccgccttcca ctcggagctt ggtgcccacc cgcgcgcgga tggacagcgt
    27721 ttttcctgtg gcggcgcttt tcttgcgcgc ggtggtgccg gccatgccgc gcgagctggt
    27781 gacggcgccg ttttcacccg tgtagatgga ccattcccga tgaacatggc cgccggcttt
    27841 ttcagccaca ggggcgcgct ctttgggaga ggcgcgcagc acgtcggcgg tcatctcatg
    27901 ccattgcgcg gacatgcgcg cgacaatgtc gggatgttgc gcggccacat cgtggagttc
    27961 cgtgcggtcc tgcgcgaggt tgtaaagctc ccagggctcg ctttgaaagg acaccagctt
    28021 ccagtcgcca tcgcgcaggg cgcggtcgct gctgaagatg aagtgaatcg gcgggcgcgg
    28081 cttgatctcg ccgccttcga gaacgggctt cagggaaatg cccgcgagtg gtgtgatctc
    28141 gcgcccagga aaactctccg gcacctgcgc gccgcagatg tccgccacag tgggcagcac
    28201 atccacgagg tgcgcgggcg tgctgacgat gctgccgggg gcggccttga tgcctgcggg
    28261 ccagtgcagg atggcgggcg tggcgatgcc gccctcgaac tggttctgct tgtagtagcg
    28321 gaagggcgag ttccgcgccc gggcccagcc ggtgctgtcg ctccaggagg tgacgccgtc
    28381 aaaaggctcc gcgtccatgc cggtgctcgc gcggtcatac gggcaggcgc cgttgtcgga
    28441 gaaaaacaag atgagcgtgt tctccaactc gcccttcgct tcgaggtcgg cgacgaggcg
    28501 gcccagctcc tggtccacgc ggtctatcat gcccgcgtaa gccgccatgc gccgcgcttc
    28561 ccagtcgcgg atttcaggct tcaggctttc ccaggccggg atgttttcgg ggcgcggcgc
    28621 gggtgccgtg ccgggtggga aaaggccaag ctcacgctgc ttggccagcc gtgccgcgcg
    28681 catcttgtcc cagccgctgt cataacgtcc gagatacttt ttatagtccg cttccagcgg
    28741 ttgcaggggc gcgtgcgggg cgttgaaggc gagatagaga aaccacggtt tcttttccgc
    28801 ccgcgcctca ttcaggaagc ccagtgcgtg gtccactttg gccacggtgg tgtaaaagcc
    28861 cgtctccggc acctgccacg gctgcccgtt caggcggaag gttttgtcac ccttgtaata
    28921 actgcacgct cccgatagat ggccgaaata gcgttgaaag ccgaagtccg tcggctgctt
    28981 cgagaggtgc cacttgcccg acatggcggt gaagtaaccg gcgggttgga gcacctcggg
    29041 caggatcacc gcgcgtgcca ggctttcatc ccccgcctgg cggcaccagc gtccgctgag
    29101 caggctgaca cgagaggagt ggcacttggc cgtgttgtaa aactgcgtga agcggatgcc
    29161 cccagccgcg agccggtcaa gcgtgggcgt ttcgatttcc gagccatagc agcccaggtc
    29221 ggagaagccg aggtcgtccg tgagaatgag caaaatgttt ggctggtccg ccgagcgcag
    29281 gctggcggtg gcaaagacga gacaggcgag aaaaagcgcg cggagcatgg cgcgtgaacg
    29341 gcaggctggc tggcgatatg tcagcaaagc cggggaacga gggtggcacg tcccatcaca
    29401 tgaataccgg agccggtgac aagagcggga ggaaagcggc gcgcggggcg tttgtttctg
    29461 ccgcatgaaa tccagcctcc gccttctgcc cctgatatgc gccgtgtccg ccgccaccgc
    29521 gcaggaaaat gatgtggcgg gcaatgagca ggtgcgcaag atcatggaga cctacaccgg
    29581 gcgcggcacc ctagccgatg acacgccgcc cacggagccg ctggaggcgg cgaggaaatt
    29641 caagctgcgc ccggacctcg ccattgacct gatggccgcc gagcccgccg tggagcagcc
    29701 gctttacgcg agctgggact cgcaggggcg catgtgggtc acgctttacc gccagtatca
    29761 atttcccgcc gggctgaaga tcgtcagcta tgaccagcac ctgcgcgcca agtttgacaa
    29821 agtgccgccg ccgccgccgc gcgggccgaa gggcgcggac aaaatcaccg tcttcgagga
    29881 cacggacggc gacggctttt tcgacaagca caccgatgtc atcaccgggc tgaacatcgc
    29941 cagcgccgcg ctgcacggca tgggccgcat ctgggtgctg aacccgccct acctgctcag
    30001 ctaccctgac gccgacttcg atgccatgcc cgatggcgat ccccaggtgg agctgagtgg
    30061 cttcggcctg gaggacacgc acagcgtggc caccagcctc atgtggggca tggacggctg
    30121 gctttacggc gccaacggca gcaccaccac gggcaacgtc tccagcgcca acaccaagaa
    30181 tgtgaaatgg gagggccagt gcatctggcg ctaccacccg cgcaagaaga ccttcgaaat
    30241 ctacgccgag ggcggcggca acaccttcag cctcgacctg gacagcatgg gccgcgtctt
    30301 ctccggcacg aacaacggca agacccgcgg catgcactac gagcagggca gctacggcat
    30361 caaaggctgg ggcaagcatg ggccgctgac caatccctat gcctttggct ggttcgagca
    30421 catgaagcat gagggcgatg aaaaacgctt cccgcaggcc tttgtcatct atgagggcgg
    30481 cctgctcggc agcgcttatg aggggaaaat catcgcgccg aactccctgc aaaacaccgt
    30541 ctatgtcagc gagcgcatcc cgcacggctc caccttccgc accaaggacg aggagctttt
    30601 gatgagctcc cccgaccgct ggttccgccc cgtgtgggcc ggtgtgggac ccgatggcgg
    30661 cttctacatg gccgactggt atgacacccg cctcagccat gtgcgccctg tggacgactg
    30721 gcacaagacc agcggccgca tctaccgcgt ccgccccgcc agcggcgcgc cgaagctcaa
    30781 gccgtttgat ctgagcaagg ccagcgcgga ggaactgttc gggtatctga gccaccccaa
    30841 ccgctggttc cgcaggcagg ccgtgctgga gatcgggtgg cgcgggctga atgacctcgc
    30901 gccaaaactc aaggacatgc ttgccggacc tcaagcgctt gaggcattat ggaccctgga
    30961 tggattggat gctgtgacgg atgacgcgct ggccggtttg ctaaggcatg ccgaccccca
    31021 tgtgcggcgg tgggcggtca agatcacggg agaaaagggc cgcgaatgga ctgccagcac
    31081 ggtcatgctg ctcgaccgcg cgggcgcgga ggatcacccc gaagtcagag cgcaactgct
    31141 cgccaccgcc aaacgcctgc ccgcgcgcgc cggcctttcc atgatctggg cggcgaacct
    31201 tcccgcgccc gatgatgaca cccacctgcc cttgcttgcc tggtgggcct tggaatccaa
    31261 ggccgaaaaa gagcgcgagg cggtcttcac ctttctcaaa tccgacaccg ccttcctcca
    31321 gactgagctc ttcaaaaacc acctcgccga gaagctcgcc aagcgctacg ccctggctgg
    31381 tggcgaggcc aatctgaact cctgcgccga cctgctcgcg ctcacggacg atgccgccct
    31441 gcgcgccaag ttcatcgccg gcatcgcctc cgctttcgaa ggctcggaac tgccgccgct
    31501 gccggaggcg ctggcgaagg cgctgaatga ttacatggcg aagcaaagcg gcggcgacct
    31561 cacgctcgcc ctgcgcagcg gcagcgcgga tgctttgaag aaaggactgg cgctgcttgg
    31621 caacagcaag gaacccaacg ccgcgcgcat cgccgtggcc agaaccctgt ccgagctagg
    31681 caaagtggag gccgtggcgc ccatggtggc cattttgaaa agcagcaacc ccgccagcat
    31741 gaagcgcggc attttgcagg ccgcctcccg ctttgacgac cgccgcatcg ccgaagccct
    31801 gctgaccagc tacgaatcgc aaatcgccgg ggaccaggca ttgcgcgagg acgccctgcg
    31861 caccctggcc gggcgcaagg aatgggccgt cgagcttatc cacttcatca acgaatggaa
    31921 agtgcccgcg cggcatgtgc ccgtggatgt cgtgcggcag ctcagcctgc acaaggacgc
    31981 tgacattgac gccgccattg agaagcattg gaagggcctg ctcgccaccg gccccacgga
    32041 ggccaaggaa aaagagatgg cccgcatcaa agctgtgctc cagaccggcc tcggcgatcc
    32101 tgaaaaaggc aagctgcaat acaccgtgcg ctgcggcatc tgccacaagc tctttggcga
    32161 cggcaatgcc atcggtcccg aactcaccgg ctacgaccgc gccaatgcca gcttctggct
    32221 tgagaacatt ttcaatccca gcctcgaaat ccgcgaaggc tttggcaact acatcgtcaa
    32281 gctcaagagc ggccagctcc tcaccggcat catggacgcg caggacgcct caggcatcgt
    32341 gctcaaagac atcgccggca acaaaacccc cgtgaagcag gccgacatcg aaaccctcga
    32401 agccagcccc gtctccctaa tgcccgaggg cctcaccacg ggcatgaccg aggcggattt
    32461 gaaggacttc tttgcgtatt tgatgaagcc gtgatgagaa acttgcgctc acgccgcgcg
    32521 ttctccaccc cggtcatgat agcacgtttc acactttccc tgctggctgc cgccagcctc
    32581 catgccgcgc cgccgaatgt ggtgctcatc gtcagtgacg accagggctg gccggatctc
    32641 ggctgcattg gcagcaagcc cgtccagacg ccgaatctcg acaagctggc tgagcgcggt
    32701 gtgcgcggca ccagctttta cgtcacctgg cccgcttgca cgccctcgcg cggcagcctg
    32761 ctcaccggcc gttatcccca gcgcaacggc ctctatgaca tggtgcgcaa tgacatggtc
    32821 aactacggcc accgctacaa acccgaggaa tacgccgtgt cgcctgagat gacactgggc
    32881 ctcgatccga gggaaatcac catcggcgac atgctgcgca aggccggcta tgtgaacggc
    32941 gtcgtcggca aatgggacat ggggcaggca aagcgttacc tgcccctcca gcgcggcttt
    33001 gatttcttct acggccacgg caacaacggc atagactact acacgcacga gcggtatggc
    33061 acaccctcac tttttcgggg caatcagcgc accgaggagg acaagggcaa atacgccacc
    33121 gaggtctttc gccgcgaggc tctttatttc gtcaacgagc acgcgggacg gaaccctttc
    33181 ttcctctacc tgcccttcaa cgctccgcac ggcgcctcca cgctagccga ggacaacggc
    33241 ggccagaagc ctggtgtcca ggcgcccgag gaatataagg cgcgttttcg cgacatcgtg
    33301 aaggatgaaa aactcgccgg ttactatggc gcggtcactt gcatggatga cgccatcggc
    33361 gcggtcatgg acgcggtgga gaaagccggg cagactaaga acacgatctg gattttcatg
    33421 tccgacaacg gcggcagcgg caacggcggc aatgcgcccc tcaaaggcag caagtccacc
    33481 atgtgggagg gtggcttgcg cgtgccattc atcttcgtct ggcctggcag ggcgcctgcc
    33541 gggaaggtca cggatgaatt tctcaccgcc cttgagatcg tgcccaccct gcttgccgcc
    33601 acgggtgctc ccgcgccaga gggtgttgtc atggacggct tcgacatgct gcccgtcctg
    33661 cgcggtgaga tgaagtctcc gcgcggcgag atgttctggc agcgccgtgg agataaagcc
    33721 gcgcgcatcg gccagtggaa atggctggaa agcgccaagg ggcgcggcct ttatgatctg
    33781 agcaccgacc tgggcgaatc ggaggatctt tcggaggaga agcccgaagt gcttgaaatg
    33841 atgcgggaga aatttgccgc ctggcgcaaa accatggacg aagccgaacc acgcggtcct
    33901 ttccgcgact attgaccgcg gcatgccacc acctctcatt ctcaccaacg ccacggtcat
    33961 cctgccgggc gggctgctgg agaatggcgc ggtcctgtgt cgtgatggac gcattgagtg
    34021 gatcggacgt gttcgaaaaa aatggcccgc aggcgcggag atcgcggacg cgcgcggcgg
    34081 gttcgtttcc cccggttttg tggacattca tgtgcatggc ggagcgggcg cggacttcat
    34141 ggacggcacg ccggaggcgg tgcgcacggt ttgcaaagcg cacgcgcgcc acggaacaac
    34201 gacgattttt ccaacgacca ccacaggcgc tccggagcag attcaggcca tgctcgccgc
    34261 ctgcgcggcg gtgaaaaaat cctggtctcc cgcggatggc gcgcgcatcg ccggagcgca
    34321 tctctacggc ccctacttcg ccgaggacaa ggtgggctgc cacagggtcg aaggccgcag
    34381 gtctcccagc cctgatgaat acgcggctta tttcaaaggc ggcctggtgc gcgtcgccac
    34441 ctgcgcggcg gagctgccgg gagcgtcatc attttacaaa gcggcccggc gcgcccgctg
    34501 tctcgtcacc tgcggacact cgaacgcctc ctggcccgag atggccgccg cgttcaaagc
    34561 gggcatgagg catgtggatc acttttggtg cgccatgagc tcggtaccct cggtgcgcgc
    34621 ccgtctcgga gtgcccatgc gcgggagcat ggcggagttt gtcctcgcgg agcgtgagat
    34681 gagcaccgag gtcatcgctg acggctgcca tcttgcctct gagttgcttg agttcgcctg
    34741 gcggatgaaa ggagccggcc gcctttgtct cgtcaccgat gccaaccgcg cactcgacat
    34801 gccgcccgga agataccgct tcggaccgga aggtgacggc tcgtggttcg agagcgatgg
    34861 cagagtggga tgggcgccca acggcagcct ggccagctcc atcatgggca tggaccacat
    34921 ggtgcgggtc atgcggcgcg atactacggc accgctgcat gacatcatcc gcatggcagc
    34981 gctcaccccc gcggagcgcg cgggcatcgc gcgcgagtgc ggcagcgtcg agccgggaaa
    35041 acgcgccgat ttgctggttc tcaaccgccg cctgcaagtg cggcgcgttt tcatcggcgg
    35101 aaccgaagtg ccctgagcca tgcttgccaa atccgaccca actttggcag acatgaccgt
    35161 cttttcccgc tcgttatcgc ccactgcaca ctccaacctt ccggcccatc atgcgtctca
    35221 tccaccttgt ctcactgctc cttctgctcg ccttcccctc ccctgccctc cttgcccagg
    35281 cggcccccaa gggcaaaggc aagggcggtg tcaaaatgaa gggcgacaaa tccgatccgg
    35341 gcaactggcc ggactccagc aagggcagcg cggcaccgca cgcgccgcag gaggagcctc
    35401 ccgccgccac agccgcccgc ccgcagtccg ccaccgcgcc tgattccggc gagcgcatcg
    35461 tcttcctcgg caacggcctc gccgagcgcg atgtctattt ctcccgcctc gaaaccgagt
    35521 tccaccttcg ctacccgaac gaaaaactgc tcatccgcaa catgggccgc cctggcgaca
    35581 ctcccggctt ccgcccgcat ccggcgcgcg cctcgcagtg ggcgttccca ggcgcggagg
    35641 tcttccatcc cgaactcgac atgcatttcg gcaagggatt cttccccact cccgaccagt
    35701 ggttgtttca cctcaaagca gacaccatcg tcgccttctt cggctacaac gagtccttcg
    35761 acggtccggc caaagtcggc aacttcgagg ctgagctgac cgccttcgtc aagcacaccc
    35821 tcggcaaggc ctacaacggc aaagccgcgc cgcgcctcgt gctcgtctct cccatcgcct
    35881 tcgaaaacct ctccgccaat cgcgaccttc ccaacggcga aaaagagaac gccaacctag
    35941 ctctttacac cgctggcatt gagcgcgtcg ccaaagcgca tggcctcacc ttcattgatc
    36001 tcttcactcc aacaaaggca ttgtattcca aagaccggtc tgctttcacc atcaacggct
    36061 ttgccccaac ggaagccggc tatcaagaaa tcgccgttca cctcgccaac ggactctacg
    36121 gccatcagtc gcatcaatcc aaggccgatc ccgccaaagt ccacgcggcg gtgaagcaga
    36181 aggattggct ttggaacaac gactacaacc tcgtcaacgg cgtccacagc cacggccagc
    36241 gctacaaccc ctacggcccg caaaattacc ccgacgaggt tcgcaagacc cgcgagatga
    36301 tgtccctgcg cgaccatctc atccacgacc tcgcctccgg caaactggcc gacatctccg
    36361 tggatgacag tgccactcac aagctcccgg ccgtgcccac caatttcaat cccaaggatg
    36421 acaagggcaa gatgggcagc atcgaataca aaaacgtcga gcaggcgctc tccagcttca
    36481 ccatggcaga aggctacaag gtggacctct tcgcctccga gtccgagttc cccgacttga
    36541 aaaacccggt gcagctcagc ttcgacaaca aaggccgcct ctgggtcgcc gtcatgcccg
    36601 gttacccgca ttggaaaccc ggtgatccgc cgcccaacga caaactcatc atcctcgaag
    36661 acaccgatgg tgacggccgc gccgacaagc agaccacctt cgccgacggc ctccagttgc
    36721 ccatcggctt cgagatcgct ccggaaggcg tctatgtctc cgaggaaccc aatttgatcc
    36781 tgcttgtgga cgagaatggc gacgacaaag ccgaccgcaa agagttgctg ctgcatggct
    36841 ttgacacaca cgacacccac cacgccatct ccgcctactg cgccgatgcc tccggcgcgt
    36901 tctacatggg cgagggccgc tttttgcata gccaagtgga gaccccctac ggcccgcgcc
    36961 gttgcaacga tggcggtgtc tggcgcttcg atcccaagtc cttccgcctg gagcgctacg
    37021 cgcaagtgga tgtctccaac ccctggggca tcagcttcaa tgactgggag cagtgccaca
    37081 tctccgacgg ttcccccggc gagagctggt ggggactgcc tgtctccgcc aagatgccct
    37141 acggcatcga gaatccaaaa gtcgggcagt ttgtgccgaa gcgctcccgc cccacctccg
    37201 gcgcggagtt cgtctcctcc cgccacttcc ccgacgagca tcagggcgcg ttcatgatct
    37261 gcaacagcat cggctttctc ggcaccagca tgggcaagat ccgcgagagc ggcgcgggct
    37321 acgagggcga gttgatcgcc gatttgctct cctccaccga tggcaacttc cgccctgtgg
    37381 acatcgagtt cgcgcccgac ggctcgctct atgtcgtgga ctggcacaac gccctcatcg
    37441 gccacatgca gcacaacgcc cgcgacccaa atcgcgacca cgaccacggc cgcatctatc
    37501 gcatcaccta tccgtcacgt cctctggttg aacccgccaa agtctttggc gcaagcatcc
    37561 ccgaactcct ggaaaatctg aagctgcccg ag
//
LOCUS       DAOMYJ010000026        37361 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Prosthecobacter sp. isolate AMR_MDS_5629
            NODE_419_length_37361_cov_21.330027, whole genome shotgun sequence.
ACCESSION   DAOMYJ010000026 DAOMYJ010000000
VERSION     DAOMYJ010000026.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867850
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prosthecobacter sp. (wastewater metagenome)
  ORGANISM  Prosthecobacter sp.
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Verrucomicrobiaceae; Prosthecobacter.
REFERENCE   1  (bases 1 to 37361)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 37361)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.30
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:34:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,742
            CDSs (total)                      :: 4,684
            Genes (coding)                    :: 4,676
            CDSs (with protein)               :: 4,676
            Genes (RNA)                       :: 58
            tRNAs                             :: 55
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37361
                     /organism="Prosthecobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_419_length_37361_cov_21.330027"
                     /isolate="AMR_MDS_5629"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1965333"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..568)
                     /locus_tag="PLP58_04530"
     CDS             complement(<1..568)
                     /locus_tag="PLP58_04530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ07810.1"
                     /translation="MNNWLHFHILLLLLSSAGLRAQESGEEASERVKYSLIFPEEKTP
                     ELVKADENNPFETVAENGLNSEGDTEENQVRDMLLAMPVGGGVSGPNGLRVMLGGMRL
                     EPGADVPPVIPDQQVRLRVHNISPSAIELVWVDKRPTGLPPKMLVIPMDGSPSVRYRM
                     PSPGRGSSGGIGTIRKDGMTALAPPRDAP"
     gene            complement(579..1124)
                     /locus_tag="PLP58_04535"
     CDS             complement(579..1124)
                     /locus_tag="PLP58_04535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ07811.1"
                     /translation="MNPKQLACVVLMMFIGGITYFGQIVHQKVVLMKNAAVDAEADAQ
                     AAESNRQTADILTARTKAETEEIRRFLKAWTPHVEKTQTEQEVESAIEFSLRDKGISL
                     VRSRKSEVKNTRENPIIPRTVIATLTIEDEYAKVHNWFGEIERRLPLSRVKVCRFTGG
                     GTGRQLRLDVSLETPLVNLAP"
     gene            complement(1140..1691)
                     /locus_tag="PLP58_04540"
     CDS             complement(1140..1691)
                     /locus_tag="PLP58_04540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ07812.1"
                     /translation="MNDYICHDFKTPRTDTSKRLPDTFKAVPIAFYLAVVGGACFMTL
                     DWLAYKKAEQAKALADSVKAGHEAVIKQLQDEKTALDAETAKAESLAKWVEGTRNVQP
                     VCVAIARAMPPEVRLTDLTLERSDQVPSNLALSIRINGGSAREVGLVDTSVSRLNYRS
                     YSPQQTKEGDLIEYRSTLVRMEN"
     gene            complement(1710..2579)
                     /locus_tag="PLP58_04545"
     CDS             complement(1710..2579)
                     /locus_tag="PLP58_04545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ07813.1"
                     /translation="MLLDEIKSVLSFAAFKPEPDDAATSWSRRSEGRKTLLINISRSQ
                     TSWRAINKRGKFDDGGVMDGEFADIAPQRAEEWRGMADGGWCVVSVNNRFILSLENNM
                     MRGENTAHLLRTNPRAVLGPKYDRGKRYALCHHPDSSASLLLACEESMVKVCEDVLKT
                     IGIRPGRVCCGLFALTEYAVHQLYVAQRGSMPADTLIISACEGSIAALVQQEGQWKEL
                     RCRSGLGPDAVETSLQIITPLLQKVPQGSPVYFISDGQDTKFRDDIFVHLQKAGAIDL
                     TQEDLLWQVIGRH"
     gene            complement(2566..3630)
                     /locus_tag="PLP58_04550"
     CDS             complement(2566..3630)
                     /locus_tag="PLP58_04550"
                     /inference="COORDINATES: protein motif:HMM:NF012652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase, T2SS/T4P/T4SS family"
                     /protein_id="HRJ07814.1"
                     /translation="MEDIHERSQLGYRMLHLSKDSGVSDFYITPWEPLTYKRNGKLFF
                     DSFIYQPERPLEFTTGCVDYALTLGNRRYRVNRMVTRGRPRWVMRLLPEEIPDISKLM
                     VPPAAIKAFLEAKNGLFLVCGATGSGKSTTIASMILERAKRRQEHVLTFEDPIEFVYP
                     PGIPSLVSQREIGTDELDFNKSLRAALRQAPDVILVGEIRDGETAEIALQAAETGHVV
                     VATLHTSSAAQTVQRYLKLIPSDRMENAMLSFADSFRGILCQRLLFDETRGKRFPVHE
                     LLLPYDSVCGMIRRGEFKNLEQELEAGFTRGMQSFERSVNMRMADGWKPTQTRKTGYA
                     EHEVYDYLSRENLTSIYAVG"
     gene            complement(3646..5181)
                     /locus_tag="PLP58_04555"
     CDS             complement(3646..5181)
                     /locus_tag="PLP58_04555"
                     /inference="COORDINATES: protein motif:HMM:NF038131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choice-of-anchor K domain-containing protein"
                     /protein_id="HRJ07815.1"
                     /translation="MELVVLIALMGVLMTVAINMVGQQPVVVKNVKLSSDVATLNHML
                     SLYQADGGSLAGMTNPEAVLTRLKRARPQAEWSSHTGPVSGRLVDNRLRARVTSSSSQ
                     PGLERARWNTRTQRFEITTGSGSAVEEFFLDEALSTSDFGTENRSAAKVRYNTSTGAN
                     QGWVWGSSPTANFAYASPGSMQGQGTSQIFDPDELVPTPPGDPGDGGSGTGGGGTGGG
                     SGVGGGGSGTTQATTLPRPVISPSGGTFSYSAFPTGVLISPNGAPPAGSTLQYRINNG
                     AWLPYEGVPVTVGSADRLQARNMAMDTALYKTSSTASAIYYRLVDSFAGTGTGTWGNA
                     NGGSNLVTVVENGIPTSTFRHGNTKLDLGSGEYLDAGIENVLSFTRESFDSVTPNTWF
                     NLGSLVMLNGTTFYDSEAGSVTLSVNLGLTQPAHTGVVHINLGLISTENTSNRQASAD
                     IVELRNPSTDFRLTLEGVEYRLELSWQSLDTNAGWVQGNQFFIYEGAAAGAQLRARFV
                     SNR"
     gene            5673..7535
                     /locus_tag="PLP58_04560"
     CDS             5673..7535
                     /locus_tag="PLP58_04560"
                     /inference="COORDINATES: protein motif:HMM:NF012485.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ07816.1"
                     /translation="MLSDVLRFLATDAGISFFSLPDDRPEGSQLITFSIRASPFQVLE
                     TLCKANELALIPDNGIWYIRPADDRELIGKSYEIRHNALERVERTSSGSGLSLTPVGS
                     NAGGGGGGSGGVNLQGNQETFQVRRSEIINAIRDLLSLPREEQEDSQQGAAAPPPAPT
                     LGSPFGGGGEETRALNSNELSAFRKPKVIWMSDSNTLYVVATRLQHMWVEGYLQVADK
                     PQSLIAIEVKFIETTRDPRRDFGVDWTGTFDVGNFRAVDTVTESVDATTGQRTVQVNY
                     NNTPTNGGYRTDLANLLSTANLNAAGAALGWPALGILSAQDINVKLRALLRDEETQMT
                     SYPRMVTLNNKEVSFRSVVNQPVLDASASATVGAGATTTTAIAYLPIGTVLNILPKRM
                     ENDKIRLNMAVTVSSIIGTEVINGNPYPVASSRVYDAPVEINSGYTVAVGGLDEARER
                     EGKSGVPILQAIPLIGKRLFSYESKSKNHKNLMIFITPTLIDARQGGLPREPQSVIPQ
                     RPNQPLPRIPQVDPNTGAVVGGPSALPNAVAFLRRETDILHHTIYESRITPDESRKVR
                     ELKVALDHLDAQCEAMKLQYPAQAAQIYAQQMQLKELLDRNQQMARLLFSKKYF"
     gene            complement(7592..8218)
                     /locus_tag="PLP58_04565"
     CDS             complement(7592..8218)
                     /locus_tag="PLP58_04565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ07817.1"
                     /translation="MKPNLLALSAGVLGVFSLSSCAVYDPYAYGAPVGGYYNGAAFAP
                     APVFSSLSVGYWGGGGNWWGNRGWWGNRGWGGGYPCYGRSNVVVNHHHHQGVTRGTTA
                     FRGIPFSSGAVPATPRFSAPVTLSRPATTRSSAPFSFTGAPAPSGRISAPQSRGSTLA
                     RPSGSSGIIRASTSSSPGRSQAISSGRTLARPSSVASRSSSSENRRSR"
     gene            8365..9246
                     /locus_tag="PLP58_04570"
     CDS             8365..9246
                     /locus_tag="PLP58_04570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009961491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase family protein"
                     /protein_id="HRJ07818.1"
                     /translation="MRYHVIHRTTCTYESPVTVCHYNARLEPRVLPTQDCPWHELTIR
                     PEPVEKAERADYFGNHSVYFEIEGSHQKLEVIARSLVEISPSDLPDPSVTPAWEMVRD
                     ACRADLPSTATAAGDFGFASQLIPVGTEFADYARASFPARRPILEAVCDLNRRIHEDF
                     IFDPSATDIATPVTEVLKNRRGVCQDFAQVMIACLRSIGLPARYVSGYLETEPPPGQP
                     KLIGADASHAWVSVFCGEAHGWMDADPTNNILPEQRHITLAWGRDFADVSPLRGVTLG
                     AGEHHLQVAVDVLPAGD"
     gene            9343..9693
                     /locus_tag="PLP58_04575"
     CDS             9343..9693
                     /locus_tag="PLP58_04575"
                     /inference="COORDINATES: protein motif:HMM:NF017868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamylcyclotransferase"
                     /protein_id="HRJ07819.1"
                     /translation="MLVFVYGTLKRGKSNHAFLDGQKFAGLAVTRAVYRMYDAGGYPG
                     LVYDVENGCPIRGEVWDVDEACLERLDVLENVAGGDYQREKIALEPPFDGLDVQGYRW
                     LLRLTGLRDAGQSW"
     gene            9768..11057
                     /locus_tag="PLP58_04580"
     CDS             9768..11057
                     /locus_tag="PLP58_04580"
                     /inference="COORDINATES: protein motif:HMM:NF024753.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HRJ07820.1"
                     /translation="MKSLYALALFPALFAHAANDWNRFRGPNGSGVSDASGLPDSVTS
                     ETTLWKVELGKGWSSPVVWEDKIFVTAETGEDSRAVICLDVKSGRELWRHEEKFAPHK
                     KHNFNSFASNTPFADAERVYVNWCTGTTIQALALDHSGRELWRNEGIANHIHEHGYGS
                     SPIVVDGVMIVRSEFDWQRDGKVYTEDPEEQKWKSCVAGLDAATGREVWKLEIPNTLN
                     TFSTPAVNVRKDGTKEIICANTTSGVFAVDPKKGVINWQYNPGFNQRNVGSGVLKEGR
                     YFCTFGTGGGVKEFAAIDVTGKEPKPVNFEFSRGLPYVPSPLVVGNLMYLLGDGGILK
                     AVKFDTGEMLYDERVNGSAGSSKFFGSPVAADGKIFCGSQQGDLIVIKQGEKFEQLSA
                     SKLDSPISSSPAISDGRLFVRTEKMLWCIGAKGAPLP"
     gene            complement(11058..11756)
                     /locus_tag="PLP58_04585"
     CDS             complement(11058..11756)
                     /locus_tag="PLP58_04585"
                     /inference="COORDINATES: protein motif:HMM:NF018645.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HRJ07821.1"
                     /translation="MLCKGLKFEISKFAVSDACPRRSMSVFLLPMQELHPLTHQGLPS
                     AVRWSHIFLESHLRAGDLAVDATAGNGHDTLFLSHLVGPKGHVFALDVQAAALESTRR
                     RLEDHGIQLNRHSLIQAGHESLLQSIPCSFHGRIKGIMFNLGWLPGSDKGIMTRAQTT
                     LPALDAALHLLAPGGLLTLAVYPGHEGGREERQAICQWADQLPIRSFEVQQIRPINPA
                     AHPPECWVFWKKKV"
     gene            12000..13067
                     /locus_tag="PLP58_04590"
     CDS             12000..13067
                     /locus_tag="PLP58_04590"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009958533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003849 - 3-deoxy-7-phosphoheptulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HRJ07822.1"
                     /translation="MSLHHPTDDLRVAEILPLIQPALLMHEYPLGEDEAAFIEESRRA
                     AEDIVKLKNDRLLVVCGPCSIHDMKSALEYSERIVEARARHAADLEIQMRVYFEKPRT
                     TVGWKGFINDPHLNESYDINNGLRGARSLLIELAKKRVPAATEFLDVITPQYIADVVA
                     WGAIGARTTESQVHRQLASGLSMPVGFKNGTDGGIQIALDAIQAAAGEHCFLSVTKDG
                     VAAIVKTQGNDACHVILRGGKGGTNFDAAAIQTVVDQLGPRGLPASVMVDCSHGNSLK
                     DYRKQPLVADSLAEQLRQGSKAITGVMIESHLVEGRQDAKPGQELVYGQSITDACVNW
                     ATTETMLEQLAQAVRERRKLG"
     gene            13083..14084
                     /gene="rfaE1"
                     /locus_tag="PLP58_04595"
     CDS             13083..14084
                     /gene="rfaE1"
                     /locus_tag="PLP58_04595"
                     /inference="COORDINATES: protein motif:HMM:TIGR02198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycero-beta-D-manno-heptose-7-phosphate
                     kinase"
                     /protein_id="HRJ07823.1"
                     /translation="MLNASTARDAIQRMSGCRILVIGDVMLDHFIWGAVRRISPEAPV
                     PIVEVTKETTFPGGAANVARNLSAFTPHAYLMGRVGKDSAAGELAKLLNEEGVNTSPM
                     LESDTLPTIAKTRIIARQQHVVRVDREMIEKLSAEELKEISRRVEAMLPDLDGLIIED
                     YGKGFVTQAFVDQVLMLAKAAGKLVTVDPSPRNPLSWRGATLVKPNRLEAFAAAGIQD
                     ARTPGPPLEDARLLEVGEQLLDQWAVEKVLVTLGEQGMMLFRRNAPPHHIPTRAREVY
                     DVSGAGDTAIAFLTLALAAGLSDEAAADIANHASGIVVGKLGTARVSVDELLQAFEN"
     gene            14093..14671
                     /locus_tag="PLP58_04600"
     CDS             14093..14671
                     /locus_tag="PLP58_04600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009958538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-sedoheptulose 7-phosphate isomerase"
                     /protein_id="HRJ07824.1"
                     /translation="MSDFSTMFQGHLDGHTATLQRLQECRDVLAAIAEAWVTALKAGK
                     KVIFFGNGGSAADAQHLAAELVVRYRANRRALPSIALTTDTSILTAHSNDYGFETVFA
                     RQVEALAQPGDVVVGISTSGTSKNVVAGLKAALEAGCVVVSFTGQNGADCAALAHHAY
                     CVPTPVTAYAQECHLMAGHVLCEYVEAAFAAV"
     gene            14739..16079
                     /locus_tag="PLP58_04605"
     CDS             14739..16079
                     /locus_tag="PLP58_04605"
                     /inference="COORDINATES: protein motif:HMM:NF024832.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flippase"
                     /protein_id="HRJ07825.1"
                     /translation="MSASSHSLRRALSGLPVSILLRVARLLVGLGLAAMLNRHLGPGG
                     AGQIMIGLAWVAPLLCLAELGFNRLTVRELAAHPDAEREILGTTFCTRIIAGIALCGA
                     AFGLSFLLEPSVRVLVWVYALLLPTHASSELLLWLESKGRLAEASWAQFRGFALAGVL
                     MIAGVIAKAPPVFFAVTYVVESWSAIAILVWMYHRQGGSMRAWRWSGARSLKMVREAW
                     PEMLAQLVMLMLFRVDTLMLGWMRGDAEAGIYSLAVRLSEVVYFIAPLLGSAFLPRLV
                     ELRKNDPAKYETRFSQYLGVNLAFASLCGLGLTLAAPWLVWLLGGKAFAGSASILMIH
                     AWGVLAYYFGVARTQYLAAEHRLHANLPAVITGLVCNVLLNLWWIPQWSGLGAAWATL
                     VSYTLGWVVITALSPGLRPLLRLQWRGLAGLPALVRESWRKRRAFWNPRPAGIS"
     gene            16076..16771
                     /locus_tag="PLP58_04610"
     CDS             16076..16771
                     /locus_tag="PLP58_04610"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HRJ07826.1"
                     /translation="MTRFLRSLLRKYCLTANTIRYRVEWPRLAAAMRVCGPVETLFDG
                     GAGSGEFARRMLDHHLCRKVIALEPETFLLLQQNLGHRSEARLIEGSLLEVPLPDACV
                     DLAMSTQVIEHIEDHERAVAEMARITRPGGWILITVPHPPEHFPQPGHVREGYTRQDL
                     ADLLSLFGCAPVHTDWFLTRPTTTRFLKACRLPLKGMYLPVAWADRETALTPEGRRLQ
                     EPFGILMLFHKRS"
     gene            16768..17934
                     /locus_tag="PLP58_04615"
     CDS             16768..17934
                     /locus_tag="PLP58_04615"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HRJ07827.1"
                     /translation="MTGALRHVLWVDPNVTQDSPSMKGLMTALPTLRELGWVFEFWCL
                     RCDAPEGFVKVRRLPAAPWLGTLENVWFVLVANICYVWRSLVFGRPAQVVGSTGAYCL
                     FADVVHAHFDSAEWLRVQARLGGMSWRDRLRRQITRIGAALEWMSWRSPWTRAVVAVS
                     EGMAGHLRQRLGNAKSVRVLANPCDTGRFHPGARGQWRETMREQLGYDARACVFLFIA
                     TGHLQRKGFWSALRVLHRLRSEGKKEIRFLVVGGSDTMWQAEKKRIGKAAPDHEAWVT
                     LAGSTTNVERFYAAADAFLLPSHFETYALVAQEALACGLPVFVTAYWGQEMYLRQGEN
                     GWLLPWDEDGMTAVLADALDQRPWQARTEHFMDAREYGRAWSQVFHELLKSPHE"
     gene            17927..19486
                     /locus_tag="PLP58_04620"
     CDS             17927..19486
                     /locus_tag="PLP58_04620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ07828.1"
                     /translation="MSEPSRGASSVLVILAGMGLALYMLTAVLLSEGNLVGQLCRFLL
                     VAGFVMGVLLPRPAFVFWLIGCGYLDLLKRLMVVSGRMFHDDLFYVLGVAPATFAGIA
                     AGALLHGLFGGVKLRSAHLGLLAAGCVMVLLSALMSLRTNGMSLRALGPDVANAGLYA
                     LLIFVVPALYQDRVSVMRLLRLLLLAWTPVMLYGVYQQVNGFQEFEIDYLKTGMSIEI
                     KQLFSDRVRAFSTLNSPTALGTIAGGLCAMALVLGWRPLNRRQTAALASPVAWMLALV
                     YAAGMMASTVRTSLMIPVAGLMAAWAFRHRGVTWMLYAVMVLAFALLVLSADFILNRI
                     SVWNDLATTGALSRMLGAEMTSIGTFSDRLMGFRNIILNPDAYTWFGYGSERGSDPSD
                     PLYNHDLISNMIVRYGCVPLAVMSLAGFVIARHVHRAVHRVRDVDDQRLAAACLGVAF
                     AVVAASLLSGNVLATFPNNVVFWLLVSITLMLSMTGVKTRAKAAPDQLEPQSADVPAA
                     VMRAHRQHIPS"
     gene            19483..20667
                     /locus_tag="PLP58_04625"
     CDS             19483..20667
                     /locus_tag="PLP58_04625"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HRJ07829.1"
                     /translation="MKPRLLLIGHTYAALENRKKIQALAAHFELVCVTSEIESRVILG
                     RPASDFDNDSDAPGRAYELVRLPRTCVKDTAFAYPGLGVVMRRMKFDVILVENEPWAV
                     ICWQARLLAGWLQSSARFVVFTWENLVRPGVKGRILSVIYRASTLTTDGYIAGNKRAA
                     EILVKHGAGKEKVLVAPQLGVDLANHHPVGEREKMRLRVVNGLPPDAFIAGYCGRLTE
                     EKGMDELLRAGETLAGEGMHLALLGAGVMRERLEQLPARHEWLHLLPPRPHFEIPDFL
                     RCLDVFVLASKPLINRDNCWEEQFGHVLIEAMACGVATLGSSSGAIPEVLGDERAVFE
                     WGNAEALTKRLRVMKNDAALRTELARAQRGRVERHYTHDAVAEHWARFLTGLHAGKEM
                     MP"
     gene            20664..21392
                     /locus_tag="PLP58_04630"
     CDS             20664..21392
                     /locus_tag="PLP58_04630"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HRJ07830.1"
                     /translation="MNYRDILYRDYSSHFGGMKAHDARVQFAQYAATYPALPERGAAV
                     LDIGCGKGEWLAWMAEQGFDSLTGVDGSVSDLEMARGHGPAKAAWVNEDGIAFLEKNP
                     RGFALIHAKDVIEHLTKEEFIRFLLAARAALKEGGRLWLMTFNAQSPMASATRYGDFT
                     HETGYTPASLAQCLRACGFGTVAVSGRHYCSLSPGGRARWMLGRCFYALARRVLRLRH
                     GAGAAPAGVDLHTALPDLFAEARA"
     gene            21389..22177
                     /locus_tag="PLP58_04635"
     CDS             21389..22177
                     /locus_tag="PLP58_04635"
                     /inference="COORDINATES: protein motif:HMM:TIGR01444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FkbM family methyltransferase"
                     /protein_id="HRJ07831.1"
                     /translation="MRRVLIYFREILVSLPLALIKGWKCFGWRGSWLAFQDLVFTRLF
                     FPVRVPGFGMLRRRNEARSLMDNFCLGELRCEEVEQVLRSHPAPVVVDLGINLGMSVR
                     WWFHLNPKARVTGFEMMQEALDYTGARLRDSHPEADWTPVCCAVSAADAEAMEVCFDD
                     ALEGTTSAVRSAGTQKRSVPARCLDSVVREDDVVLLKCDIEGFGGHALEGARAVLKRT
                     RYVVTETHGPEEMERMSRVLFEEGFAPFKLAARSLWWRSLEFKL"
     gene            complement(22258..23256)
                     /locus_tag="PLP58_04640"
     CDS             complement(22258..23256)
                     /locus_tag="PLP58_04640"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HRJ07832.1"
                     /translation="MNLLIFKVNQLGDNVVFLPLVQQLAAHFPHWRITVMTSPAAAPL
                     YEVACPEARLLSFNTQDFNSSWKNPARLTRLLRITRALKPDTCLLGNDQGNAAHLLAR
                     LSRAALCVGPHVPERKLCFLLHHREPLIETDSAARQNWRIAQAMLARLGHPALPDAIP
                     APDLSAFGREGHVAIAIHAGASRAYKRWPLERFIALANKLADTHPVLWFDQNEAGEEM
                     LAPRIRRVTPGSLHDFIRHLAGARHFIGNNSGPMNLASALGIPGTIFNGPSRPNWDPM
                     WHSEKFDLLRDAALACQPCDLFSRPVNHCQNTFHPMACMDRWSVDEVHQRVLARLE"
     gene            23430..25115
                     /locus_tag="PLP58_04645"
     CDS             23430..25115
                     /locus_tag="PLP58_04645"
                     /inference="COORDINATES: protein motif:HMM:NF021254.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exosortase/archaeosortase family protein"
                     /protein_id="HRJ07833.1"
                     /translation="MKTGIAELRLPLASAVLCLAALVGALPYAAGYGDFRLTIWQELV
                     IRWKDPTWQHGFLAPFIAGWLVWRMREEIATWPLRAHGSGLVVMALALLSYFAGYKAH
                     NFYLGALGMQGFVLAVMLGLWGWARTRRLGFAWLMLGFMWPLVFMEQGVAYQLRVLTV
                     SGVSWVLNHTGVETVRQGTALLSAPDAARGLGELFSLKVDGPCSGMRSLFALLMVSAL
                     FGYFRQGTWPRRWVLFACSVPLAVVANMARIFLLLGGTVLFGQDFAVGNEEQEVSTFH
                     FVSGIAVYLVALAGLQGVAALMDRIWKGGAGWTRKPGRVSERAALAVESKRRMVWQGA
                     AMLAMTTVALAVCRLSPELSAGSEAGVVMTLPERTSLFMGRSEKPDTVEKTLLPADTQ
                     IVKMRYATPRPPEERDRVDVTLVLAGAERRSIHRPEVCLDGQGWTILEQRVVPVELPG
                     GCVLEVKDLLIEREWLDDAGGRRMLRAHYVYWFVGSDVSTPNNWTRVWLSSWDNVLRS
                     VNHRWAYPSLTAWVTEGWDPALTGQRQRTSEQTLELIKNLIRELAPGFQKGLM"
     gene            25118..27022
                     /gene="asnB"
                     /locus_tag="PLP58_04650"
     CDS             25118..27022
                     /gene="asnB"
                     /locus_tag="PLP58_04650"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR01536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HRJ07834.1"
                     /translation="MCGFVGCFHASGGGLDVPALRRMLAAVRHRGPDDEGLHGLSFHR
                     RESLPLDQISEARELDGGFGFARLSIQDLSAAGHQPMTSADGKVVLVFNGEIYNAGEH
                     RETLRAQGVEFRGHSDTEVILRLYEKHGWQGMLDRLEGMYALAIADVRAGVVRVARDP
                     FGIKPLYYWNRNGTLLIASEMKAFLEHPAFQAEVETEYLAEQLLFRHTAWDRTLLRGV
                     MQLPPGHTLEWRRDGTLARRRFWSLPDAEDGVVDLCQLRARLRGAVESQLVSDVPLGT
                     QLSGGIDSSLVTLDAARASAERLRTFSIVFENDAVSEDAWISQAARRAGVHSHRLLFT
                     ASDFAAGFEKAVWHLDGPPGNPNTLALQLLAREAGRHVKVLLTGEGCDELFAGYNRFY
                     YTALQQAWPGVFKAGVQFAGSGRGRWWRLFGRAGESLEHQMVISSAYGASGLVGSLLP
                     GVDPEMAITARLDHLSGIPGAGVRRQVRYEAETYLPDLLLRQDKMTMAHGVECRVPFL
                     DRSLAAAARRLPLDWLARPCLGANARMRGTKRAVKQLAQDEFGRAFAYRRKRGFDVPL
                     AEFFLHSEVRGRVEEEWLPALRDQGFLDYRAVEKEWRSLLEGRGTGAAVETLWVAMGL
                     TAWVKMIKKS"
     gene            27078..27878
                     /locus_tag="PLP58_04655"
     CDS             27078..27878
                     /locus_tag="PLP58_04655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ07835.1"
                     /translation="MKKIWLAILALTISTGLLSAQVTATVTGVEGNGFAGVYDVNGTN
                     YWWMCIEPNGTPNAALGESYTANWFWFEDGWDQQNNERFDFFNVQNPAYQTTVIPKQV
                     TVMTYVLDTYLPWDTLAGASGRFIEQDSDPLNYENNTDFYNSLFVVQNFLSEMYGKPH
                     QNDFTDLSNFVNNWDGDVTATGIARQALFQSILDDVEGKDGVNHFDTYTAIHGYSTLS
                     VRLDEADPDNWQDVLVITSFAPVPEPSGALLIACCGVAVMLRRFRRLA"
     gene            27935..28366
                     /locus_tag="PLP58_04660"
     CDS             27935..28366
                     /locus_tag="PLP58_04660"
                     /inference="COORDINATES: protein motif:HMM:NF025056.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003953 - NAD+ nucleosidase activity
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="toll/interleukin-1 receptor domain-containing
                     protein"
                     /protein_id="HRJ07836.1"
                     /translation="MQVFISHSYQDAELARKLAGSLRENGLRPWLAEEEVFPGDNWGE
                     VTGRALSESDAMVALVTPVSGAAPQVRQDIAFALTRKAYANKVIPLIVGNRDDVPPNA
                     LPWIMNRYKMVEIPSGDQMPMAAEQIVGALRGHESMLETAA"
     gene            28363..28782
                     /locus_tag="PLP58_04665"
     CDS             28363..28782
                     /locus_tag="PLP58_04665"
                     /inference="COORDINATES: protein motif:HMM:NF025056.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003953 - NAD+ nucleosidase activity
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="toll/interleukin-1 receptor domain-containing
                     protein"
                     /protein_id="HRJ07837.1"
                     /translation="MRGPVEVFLSHSSDDAETAIEIARVLGEHGISAWYAPREIVGSQ
                     KWQDEIGAALRRCDWFVLLLSPGAVRSMWVRRELQYALIQSRYDGRITPVLISPCDPD
                     NLSWVLQTFQQVDLTRRTEGAYSNLLRVWGKEFGPVA"
     gene            28806..30191
                     /locus_tag="PLP58_04670"
     CDS             28806..30191
                     /locus_tag="PLP58_04670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ07838.1"
                     /translation="MSLEDRLYPLLKLYDAAPQAVKSLAGRVYRAIPPGLRHGSAYPR
                     FLDEAREVESWDGETVERYQIRALRESLIAASKAPFYARRFAESGVRPEKFESLEQLA
                     DYPLLTKQDLISQREQMLNPEFGPEHGLHITTGGSSGVPVGFDLHKGVSRPKEQAYLE
                     AQWARRGYRAGDRVAVIRGGVTSSQASGGISHHDATRDWLILSSYHLTPERLPEYVEE
                     LNRFQPRHLHAYPSSALMLARGMEQAGLRLDFPLTSLLCGSEKLTAGSQCELEQFFGT
                     RVFHWYGHSERVVLAAQGRESNMLHFWPTYGFVEFGEPDAEGNCEIIGTSFHNHVMPL
                     IRYRTGDFCKPTLEAREFGWPAVEAVVGRDYEFLVSGTGRRISLTAINMHDRLFDGLL
                     AVQFFQEQPGAVECRLQPGPHWQSARLEEIRAGLLAKLGDDFTLTLREVGEVEKTPAG
                     KHRWLVTTLKQ"
     gene            30203..33073
                     /locus_tag="PLP58_04675"
     CDS             30203..33073
                     /locus_tag="PLP58_04675"
                     /inference="COORDINATES: protein motif:HMM:NF012335.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HRJ07839.1"
                     /translation="MRQLAQYQDGRLELQEVPAPVPPPGGILVRTTCSVISPGTERMK
                     VEQARMSLLQKAKARPDQVKKVIDTAKTLGWKAALAKVRNRLESPTPLGYSAAGVVVA
                     VDELNTRFRVGDRVACGGAECAFHAEMIAVPDLLAAPVPEGVEDWQAACMTLASISME
                     AVRQSGARLGERVLVLGQGLVGLLATSLLKASGARVMGVDLVAERLQTSLAMGAERVA
                     NPLQSSLADEVREWTAGQGVDAVLLCAGGKGRDAADTAIECLRDRGVMVIVGIYDAEL
                     SWKTAYMKDIQVRYSRSYGPGRYDPQYEWGGQDYPIGHVRWTENRNFEACLELMRTGQ
                     LDLGPVMTRRVAFEDVVGVYDALECEVGVVVEYAGRTGILPVAGESGILPDVGKPGRM
                     PGSHESGGTPDLRAFIPFDPTKKTEKARRNLPHWEQEGVTQFVTFRQADAISQEKLAE
                     WRAEREVWLREHPQPWDFETEELHQRLFGDRVEQWLDQGAGSCILAREDAARVVEKAM
                     RHFDGERYILDAYVIMPNHVHVLLKPLAGHSLSEIMHSWKSFTSREIKKLYGLGEEAF
                     WQAERFDHALRSEAAFEAKREYIRRNPVKARLNGGFVVGCGIGVASRAGILPVAGASG
                     ILPDDGEAGRMPGSHESGRMPDLHVIGAGNFARTMLLPHLKGRIGFGTVVNSTGLSAR
                     HVREKFGFAAAETDAVAILAQAGGAVLISTRHHLHAPLVMKALHSNKHVFVEKPLCLT
                     REELAELDMMMQGTQGSVMTGFNRRFAPATLAMMETLQNVSGPKTLAFHVNAGVLAPD
                     HWYANAAESGGRVLGEACHFFDFACHLLGRPVKVTAQTVGRPKAPDSVTAQVEFADGS
                     SAQIIYSAEGDSAFPKESLRVFAGGLVMECENFMRLVIYRNRKQTVRKFSSKGHAEEM
                     AAWLAFLQGKAAHPLPYEQSRQSMRLTFAALDSIREARSIPV"
     gene            33081..34916
                     /locus_tag="PLP58_04680"
     CDS             33081..34916
                     /locus_tag="PLP58_04680"
                     /inference="COORDINATES: protein motif:HMM:NF019552.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heparinase II/III family protein"
                     /protein_id="HRJ07840.1"
                     /translation="MSTGGRLFWYAHRLRAMSAAEIMHRMAERARRFTLGVCLKRAAG
                     IQPGPVNARAPFLPDPAAAPEGMREALAAEAAALQQGRWKLFGWHEAEVGAPPCWHRD
                     ARCGVVIEPKTAAHRLDHRNLPDGADARTIWEINRWAEMTRLAMHGWLNADKAAIRAA
                     QLWLEDWLDHNPTGMGINWTSPLEAALRLLNFCWFDALARAAGADTAVQDKLARRIVP
                     IHACWVWRHRSHGSSANNHLLGELAALVVALKRWPGMEKHVLPVEQVWDMLGAEVLAQ
                     FAEDGGSREQALHYHLFAFDLAWQAARAVGCRAGEVHDRLAEAADYFRCLAHPDEPWD
                     FGDNDDARVVPVARSRERAVAEWRAWLGGEPGDLRFWLGRSPSSPARVEEAGWRCFPE
                     SGMAVGRMGGWMARLDASPLGFGSLAAHGHCDVLHLSVWDGAHALLIDPGTGGYHGFA
                     GLRAELAAWEAHNGPLPAGGYRTPRRVGAFLQTAHHPQPLLAAEGGREVARFAHEGRI
                     MQRTVVMNADGIEIQDVEAAGRPLVVRWRLAPGMAAKMLANQGRACLELSLKGVLWVM
                     DPGADEANVRLAEKRASPFYAEIASSVEVEVRCAGGSLTTRLRRG"
     gene            complement(35057..35197)
                     /locus_tag="PLP58_04685"
     CDS             complement(35057..35197)
                     /locus_tag="PLP58_04685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ07841.1"
                     /translation="MTRLLTLALVSASLVLASCASKKSDCATCCDSGGKKAASCCPTA
                     KP"
     gene            complement(35485..36978)
                     /locus_tag="PLP58_04690"
     CDS             complement(35485..36978)
                     /locus_tag="PLP58_04690"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HRJ07842.1"
                     /translation="MHALRLLSLILLLPAAAAAKPNILFIFSDDQSYKTVSAYPEALP
                     GVSTPNLDALARSGIRFTHAYMGSWCMPSRASLLTGLHPHSIETMRMEGPYPGSAYDP
                     DQCRFWPATFRKAGYHTAQIGKWHTGTDTGWGRDWDYQKVWNRPLHTGNAGAYYEGQR
                     FTENGRDLGILEGYSTDNYTDWACDFIQGKNRPADKPWYLWLCYGAIHGPTTPAERHK
                     GKHKNDEVRVPADIIGSRPGKPAWLEKTQAWKMGPDGKIVGAKGKGGTTFQKFVQQSN
                     DCVEALDEGVGRLITALKESGQIENTLVVFTADQGFALGEHGLRIKLAPYDAAVRSPF
                     IISMPGRLAQGKVCAQPVNSPDLVRTFFSFAGLEPEWEMHGRDLTPLLTAPDAPAAIT
                     PCLYEHTGKYYGSEVAKVLRATPAEAEHGGVPWYVALNDGRWKYIRYLRAGEIEELYD
                     LKDDPEELANLAASAAHRPELERLRAALATELDRTRAPFTGDLNLAR"
     gene            37154..>37361
                     /locus_tag="PLP58_04695"
     CDS             37154..>37361
                     /locus_tag="PLP58_04695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ07843.1"
                     /translation="MRKLFWFNAKSNSEIARQVSREEKQERRIVANECQAGESEPRIW
                     VHGIAFPRSAGVFWRGGRRGLDPAV"
BASE COUNT         6527 a        10130 c        12980 g         7724 t
ORIGIN      
        1 gtggagcatc cctgggcggg gctagcgcgg tcatgccgtc cttgcggatg gtgccgatgc
       61 cgccgcttga gccgcgtccc ggcgaaggca tgcggtaacg aactgagggg ctgccatcca
      121 tggggatgac aagcatcttt ggcggcagtc ccgtcgggcg cttgtccacc cagacgagtt
      181 cgatggcgga gggggagata ttatgcacgc gcaggcgcac ctgctggtcg ggaatgaccg
      241 gtggcacgtc ggcaccgggt tcgagtctca tgccgccgag catgacgcgg agtccgttcg
      301 gccccgagac tccgccgccg accggcatgg ccaggagcat gtcccgcacc tggttttcct
      361 cggtgtcgcc ttcggagttg aggccgtttt cagcgacggt ttcgaagggg ttgttttcgt
      421 ccgccttgac gagttcgggg gttttttcct ccgggaaaat gaggctgtac ttgaccctct
      481 cgctggcttc ctctccggac tcctgcgcgc gcaggccagc ggaggaaagc agcaggagaa
      541 ggatgtggaa atggagccag ttgttcatgg acgcggagtc atggggcaag gttcacgagc
      601 ggggtttcga gggacacatc cagccggagc tgcctgccgg tgccgccgcc tgtgaaacgg
      661 cagactttga cccgtgagag cggcaggcgg cgctcgattt cgccgaacca gttgtgaact
      721 ttggcgtatt cgtcctcaat ggtgagagtg gcgatgacgg tgcgcgggat gatggggttt
      781 tcacgggtgt tcttcacctc gcttttgcgc gatcgcacga ggctgatgcc cttgtcgcgc
      841 aggctgaatt cgatggcgct ctccacttct tgctcggtct gggtcttctc cacatgcggt
      901 gtccaggctt tgagaaagcg gcggatttcc tcggtctcgg ccttggtgcg cgcggtgagg
      961 atgtcggcgg tttggcggtt gctctcggcg gcctgggcgt cggcctcggc gtcaacggct
     1021 gcgtttttca tgagcacgac cttttgatgg acgatctgcc cgaagtaggt gatgccgccg
     1081 atgaacatca tcaggacgac gcaggcgagt tgcttggggt tcatgatatt gcgcggcttt
     1141 cagttttcca tgcggacgag ggtggagcgg tactcgatga gatcgccttc cttggtctgc
     1201 tggggcgagt aggagcggta gttgaggcgt gagacgctgg tgtcgacaag gcccacctca
     1261 cgcgcgctgc ctccgttgat gcggatggac agggcgaggt tggatggcac ctggtcgctg
     1321 cgctccaggg tcagatcggt gaggcgcacc tcgggcggca tggcacgggc gatggcgaca
     1381 cagacaggct ggacgttgcg cgtgccttca acccatttgg caagcgattc agccttggcg
     1441 gtctcggcgt cgagggcggt tttttcatcc tggagctgct tgatgactgc ctcgtgcccg
     1501 gctttgacgg agtcggccag cgctttggcc tgctcggctt ttttgtaggc gagccagtcg
     1561 agcgtcatga agcaggcgcc cccgacgacg gcgaggtaaa aggcgatggg tacggccttg
     1621 aaggtgtccg gcaggcgctt cgaggtgtca gtgcgcggag ttttgaagtc gtggcagatg
     1681 tagtcgttca ttggggtgat gtgctgggat caatgccggc cgatgacctg ccagagcaga
     1741 tcctcctggg tgaggtcgat ggcgccggct ttttgaaggt ggacaaagat gtcgtcgcgg
     1801 aacttggtgt cctggccgtc gctgatgaaa tagacggggc tgccctgagg cactttttga
     1861 agcaggggag tgatgatttg gagggaagtc tcgaccgcgt cgggccccag tccgctgcgg
     1921 cagcgcagct ctttccactg cccttcctgc tggactagcg cggcgatgga cccctcgcac
     1981 gcgctgatga tcagggtgtc ggcgggcatg ctgccgcgct gggccacata gagctggtgg
     2041 acggcgtatt cggtgagggc gaaaaggccg cagcagacgc gccccggccg tatgccgatg
     2101 gtcttgagca cgtcctcgca gactttgacc atgctctcct cgcaggcgag caggaggctg
     2161 gcggagctgt cgggatgatg gcagagcgcg tagcgtttgc ctcggtcgta tttggggccg
     2221 agcacggcgc gggggttggt gcgcaggagg tgggcggtgt tttcgccgcg catcatgttg
     2281 ttctccaggg agaggatgaa gcggttgttc acggacacga cgcaccagcc gccgtcagcc
     2341 atgccgcgcc attcctcggc acgctggggg gcgatgtcgg cgaactcgcc gtccatgacg
     2401 ccgccgtcgt caaatttgcc gcgcttgttg atggctcgcc agcttgtctg gctgcggctg
     2461 atgttgatga gcagggtctt gcgcccctcg gaccggcggg accaggaggt ggccgcgtcg
     2521 tcaggctcgg gcttgaaggc ggcgaagctc aggacagatt tgatttcatc caacagcata
     2581 aatggaggtc aggttttcgc gtgagagata gtcatagacc tcatgctcgg cgtagccggt
     2641 tttgcgggtc tgggtgggtt tccagccgtc ggccatgcgc atgttcacgg agcgctcgaa
     2701 gctctgcatg ccgcgggtga agccggcctc gagctcctgt tcgagattct taaactcgcc
     2761 gcggcgaatc atgccgcaaa cggagtcata aggaaggagg agttcgtgga cagggaagcg
     2821 cttgccgcgg gtctcatcga acaggagacg ctggcagagg attccgcgaa agctgtcggc
     2881 gaaggagagc atggcgtttt ccatgcggtc actggggatg agtttcagat aacgctgcac
     2941 ggtctgcgcc gcgctggagg tgtgcagagt ggcgacaacg acatggccgg tctcggcggc
     3001 ctggagggcg atctcggccg tttcgccgtc gcgaatttca ccgacgagga tgacatccgg
     3061 cgcctgtcgc agggcggcgc ggagggattt gttgaagtcg agttcgtcgg tgccgatctc
     3121 gcgctgggag acgagcgagg ggatgccggg cggatagacg aactcgatgg gatcctcgaa
     3181 ggtgagcaca tgctcctggc ggcgtttggc gcgctcaagg atcatggagg cgatggtggt
     3241 ggatttgccg gagcctgtgg cgccgcagac gagaaagagg ccgttttttg cctccaggaa
     3301 ggctttgatg gcggcaggcg gcaccatgag cttgctgatg tccgggattt cctccggcag
     3361 caggcgcatg acccagcgcg ggcgtcctcg cgtgaccatg cggttgacgc ggtagcggcg
     3421 gttgccgagg gtgagggcgt agtccacgca gccggtcgtg aactccaggg ggcgctccgg
     3481 ctgatagatg aaggagtcga aaaagagctt gccgttgcgc ttgtaggtga gcggctccca
     3541 gggtgtgatg tagaaatcac tcacacccga gtcctttgaa aggtgaagca ttcggtagcc
     3601 cagctggctg cgttcgtgga tgtcttccat gtcggttgtc tggattcaac ggttgctgac
     3661 aaagcgcgcg cggagctgcg cgcctgcggc ggccccctca tagatgaaaa attggttgcc
     3721 ctgcacccag ccggcgttgg tgtcgaggct ctgccaggag agttcgaggc ggtattcaac
     3781 gccctcgagt gtgaggcgga aatcggtgct gggattgcgc agctcgacga tgtccgcgct
     3841 ggcctggcgg ttgctggtgt tttcggtgct gataaggccg agattgatgt ggacgactcc
     3901 ggtgtgggcg ggctgcgtga gtccgagatt gacggagagg gtcacactgc cggcctcgct
     3961 gtcatagaat gtggtgccat tgagcatgac aaggctgccg aggttgaacc aggtgttggg
     4021 agtgaccgaa tcgaagctct cgcgtgtgaa ggagagcacg ttttcgatgc ccgcgtcgag
     4081 atactctccg ctgccgaggt cgagcttggt gttgccgtgg cggaaggtgg aggtggggat
     4141 gccgttttcc acgacggtga cgagattgga gccgccattg gcgttccccc aggtgccagt
     4201 accggtgccg gcgaagctgt caacgagccg gtagtagatg gccgatgcgg tgctgctggt
     4261 tttgtatagc gcggtatcca tcgccatgtt ccgcgcctgg agccggtcgg ctgatcccac
     4321 ggtgactggc acaccctcat agggcagcca ggctccattg ttgatcctgt attgcagcgt
     4381 ggagcctgcg ggcggcgctc cattcgggct gatgagaaca ccggtcggaa atgctgaata
     4441 ggaaaaagtg ccgccactgg ggctgatgac aggacgcggc agagtggtcg cttgcgtggt
     4501 tcccgatccc ccgcctccga ctccgcttcc ccctccggtg cctcctccgc ctgtgccgga
     4561 gcctccatca ccaggatcac ctggcggggt tggcacgagt tcatcggggt caaaaatctg
     4621 actcgtgccc tggccttgca tgctgcccgg agacgcataa gcgaagtttg ccgtcggtga
     4681 tgaaccccac acccatcctt ggttggcgcc ggtgctggtg ttgtagcgca ctttggcggc
     4741 ggagcggttt tccgtgccaa agtcagaggt cgagagcgcc tcgtcgagga aaaactcctc
     4801 gactgcggag cccgatccag tggtgatctc aaaacgctgg gtgcgcgtgt tccagcgggc
     4861 gcgttccaga ccgggctggg aggagctgct ggtgacacgc gcccggagcc ggttgtccac
     4921 cagacgaccg ctgacaggtc ctgtgtgcga actccactct gcttgcggcc gggcacgttt
     4981 cagacgggtc agcacggcct cagggttggt catgccggcg agtgatcctc cgtcggcctg
     5041 ataaagcgag agcatgtggt tcaatgtggc cacgtcggag gagagtttca catttttcac
     5101 caccacaggc tgctgtccga ccatgttaat ggctaccgtc atgaggactc ccatcagggc
     5161 gatgaggacg accaattcca tgactgaaaa cgcgccagcc aaatgccaaa atctggcatt
     5221 atgtgaagcg gggcgctcgt tgcttgaaaa catggaaaat atgaaagata tttaaattta
     5281 ccaaaattgt tttcgatttg caatcaaaag ctgcaaaaac cacaatttag actgtcgaac
     5341 atagttcata ctcaattgtt gagagtccct tgaattttcg agacaggcca gacgagaagc
     5401 ttaacaagtt cgaaaattcg tgaaacggta gttttaaata ctatttgaat gaaatggaat
     5461 gccgggctat aatttccata tttatgatct atttcatgcg aaaatgtggc ttccttttca
     5521 ctttggcgct ctttgcgagt tggggagggc tttcaagaac tgcctgggcg caaagcccat
     5581 caggctcctt caatttttct tccgcagtct atgaatccga ggccgcaccc ctgagggccg
     5641 cgcaaccgca gcagtatgat ttttcaaaag ccatgctctc ggatgtgctg cgctttctgg
     5701 cgacagatgc cggcatctcg tttttttccc tgccggatga ccggcctgag ggatcgcagt
     5761 tgatcacttt ttcgattcga gccagccctt tccaagtgct ggaaaccctg tgcaaggcca
     5821 acgaactggc gctcatccct gacaatggca tctggtacat ccgacctgcg gatgatcgcg
     5881 aactcatcgg caagtcctac gaaatccggc acaatgcgct ggagcgggtc gagcgcacca
     5941 gcagcggttc gggattgagc ctgactcctg tcggttcgaa cgcgggaggc ggtggggggg
     6001 gatcgggcgg agtcaacctc cagggcaatc aagagacttt ccaggtcagg cgcagcgaga
     6061 ttatcaatgc gatccgtgac ttgctgagcc tgccgcgtga agagcaggag gactcccagc
     6121 aaggcgcggc ggcgcctcct ccggctccga ccttgggcag tcccttcggc ggcggagggg
     6181 aagaaacccg cgctttgaac tcgaacgagt tgagcgcctt ccgcaaaccg aaggtcattt
     6241 ggatgtcgga ctcgaacacg ctctatgtcg tcgccacgcg cctgcagcac atgtgggtgg
     6301 agggctacct gcaggtcgcg gacaagccgc agtcactcat tgccatcgag gtgaaattca
     6361 tcgaaaccac ccgtgatccg agacgcgatt tcggtgttga ctggaccggc acctttgacg
     6421 tgggcaactt tcgcgcagtg gacacagtca ctgagtcggt ggacgcgacc acaggccagc
     6481 gcaccgtgca ggtgaattat aacaacaccc ccaccaacgg cggctaccgc accgacctgg
     6541 cgaacctgct ttccacagcc aatctgaacg ccgcaggcgc cgcgcttggc tggcccgcgc
     6601 tgggcattct cagcgcgcaa gacatcaatg tgaagctgcg cgccctgttg cgcgacgagg
     6661 agacccagat gacctcctat ccgcgcatgg tgacgcttaa caacaaggag gtttccttcc
     6721 gaagtgtcgt caaccagcct gttctcgatg cctccgcgtc tgccacggtt ggcgccggtg
     6781 ccaccaccac caccgccatt gcctacctgc cgattggcac tgtgctcaac atcctgccca
     6841 agcgcatgga gaacgataaa atccgcctca acatggccgt caccgtttcc agcatcatcg
     6901 gcaccgaggt catcaacggc aatccctacc cggtggcctc ctcccgggtt tatgatgctc
     6961 ctgtcgagat caactccggc tacaccgttg ctgtaggcgg tcttgatgaa gcccgcgagc
     7021 gcgagggcaa atccggcgtg cccatactgc aggccattcc gttgattggg aaaaggctgt
     7081 tcagctatga gagcaagagc aagaaccaca aaaacctcat gatcttcatc acgcccacgc
     7141 tgattgacgc gcgccagggc ggcctgccgc gcgagccgca gtctgtgatt ccgcagcgtc
     7201 cgaaccagcc tttgcccagg ataccccaag tggacccgaa cacgggcgcg gtcgtcggcg
     7261 gtccttcggc tcttcccaat gcggtggctt tcctgcgcag ggagacggac attctccatc
     7321 acaccattta cgagagccgc atcacgcctg atgaaagccg caaggtcagg gaattgaagg
     7381 tggccctaga ccacctcgac gcgcagtgcg aggccatgaa gctgcaatac ccagcccagg
     7441 ccgcgcagat atacgcgcag cagatgcagt tgaaggagct gctggaccgc aaccagcaaa
     7501 tggcgcgcct gcttttctcg aaaaaatact tctgagcatg aaaaagccgc cattcgcaca
     7561 aatggcggct ttgtttgatt cacccgaaaa atcagcggct gcggcgattt tctgaggatg
     7621 aggagcggga agccacggat gacgggcgag ccagagttct accgctggaa atcgcttggg
     7681 aacgtcctgg acttgaggag gtggacgcac gaataatgcc cgaactgccg gaaggccgcg
     7741 ccagagtgct gccgcgtgac tggggtgccg agatgcgccc ggaaggagca ggcgcgcctg
     7801 tgaagctgaa gggcgcggaa ctccgggttg tggcgggtcg tgacagagtg acgggcgcag
     7861 agaagcgcgg agtggccggc accgctccgc tggaaaaggg aatgccgcgg aaggctgtcg
     7921 tcccgcgggt gacgccttga tgatggtgat gattcaccac cacgttcgag cgaccatagc
     7981 acgggtatcc gccgccccat ccccggttcc cccaccagcc gcgatttccc caccagttgc
     8041 caccgccgcc ccagtagccg acggacaggg aggaaaaaac aggcgcgggc gcaaaggcgg
     8101 ctccattgta atagccgccg acgggcgcgc cgtaggcata cggatcatac accgcgcaag
     8161 acgagagtga aaaaacgccg agcacaccgg cggagagggc gagtaggttg ggtttcatgc
     8221 gagtagtgac gcgccccgcg ccggccctat tcaaactaaa ttttccgctg tttttgcgac
     8281 tcctggcctg tccgctgctg ttcgctaaac gcttcccgtt ccttgcccga cagcctttga
     8341 gcgagtatgg ggcggcgctg acacatgcgt tatcacgtca tccaccgcac cacctgcacc
     8401 tacgagagtc ctgtcacagt ctgccattac aatgcgcggt tggagcctcg cgtgcttccc
     8461 acccaggact gcccgtggca tgagttgacg attcgtccgg agcccgtgga aaaggcggag
     8521 cgcgctgatt attttggaaa ccacagtgtc tatttcgaga tcgaaggctc ccatcagaag
     8581 ctcgaagtta ttgccagaag cctggtggaa atctcccctt ccgatctgcc cgacccatcc
     8641 gtcactcccg cctgggagat ggtgcgggat gcatgtcgtg ccgatttgcc ttccactgcg
     8701 acggctgcgg gggattttgg cttcgcctcc cagcttattc ctgttggaac ggagtttgcc
     8761 gattacgccc gcgcctcctt tcccgctcgt cgtcccattc tggaggctgt gtgcgacttg
     8821 aaccgccgca ttcatgagga ttttattttt gatccctcag ccaccgacat cgccacaccc
     8881 gtgaccgagg tattgaaaaa ccgccgtgga gtctgtcagg acttcgccca ggtcatgatc
     8941 gcctgtcttc gttccattgg tctgcccgcg cgctatgtca gcggttattt ggaaacggag
     9001 ccgccaccgg ggcagcccaa gctgatcggc gcggacgcct cccacgcctg ggtgtcagtt
     9061 ttctgcggcg aggcccacgg ctggatggac gcggatccca ccaacaacat cctgccggag
     9121 caaaggcaca tcacacttgc ctgggggcgt gactttgccg atgtcagccc gctgcgcggc
     9181 gtcactctag gcgcgggcga gcatcatctt caggtggctg tggatgtgct tcctgccggg
     9241 gattgaggcg catccgttcc gggccggcgt gtgcattttt tcatccgggt catcctgcaa
     9301 agcgcgcttt gacttttgcg ggattccagg acatggcggg gcatgcttgt ttttgtttat
     9361 ggcactttga aacgcgggaa aagcaatcat gcttttcttg acgggcagaa atttgccggc
     9421 ctggcggtga cgcgggcagt ttaccgaatg tatgacgcgg gcgggtatcc aggactcgtt
     9481 tatgacgtcg aaaacgggtg tccaatccga ggggaagtat gggatgtgga cgaggcatgc
     9541 ctggagcggc tggatgtgct ggagaatgtg gcgggcggcg attatcagcg cgagaaaatc
     9601 gcattggagc cgccgttcga tggattggat gtgcaggggt atcgctggct gcttcggctg
     9661 acggggctga gagacgcggg ccagtcctgg tgatgctcgc ggcttttgag aataaaggaa
     9721 agatacgccc tccgcctgcc gataaaccgc attcatatcc gtcattcatg aaatccctct
     9781 acgctttagc cctgtttcca gccttgtttg ctcacgccgc gaacgactgg aaccgtttcc
     9841 gcggtccgaa tggcagcggc gtttccgatg cgtccggtct gccagactcg gtgacatccg
     9901 agacgacact ctggaaggtt gaactgggca aaggatggtc ctcgccggtg gtctgggagg
     9961 acaagatttt tgtcaccgcc gagacgggtg aggacagccg cgcggtgatc tgcctggatg
    10021 tgaaatccgg cagggaattg tggcgtcacg aggaaaagtt cgcgccgcac aagaagcata
    10081 atttcaacag ctttgccagc aacacgccat ttgccgatgc cgagcgagtt tatgtgaact
    10141 ggtgcacggg cactaccatc caggcgctgg cactggatca ctcgggcagg gaactctgga
    10201 ggaacgaagg tatcgccaac cacattcatg agcacggata tggttcgtcg cccatcgtgg
    10261 tggatggtgt gatgatcgtg cgcagcgagt tcgactggca gcgggatggc aaggtctaca
    10321 ccgaggatcc ggaggagcag aaatggaaga gttgtgtggc ggggctggac gcggccactg
    10381 gcagggaagt ttggaagctg gagattccca acacgctgaa cactttttcg acacccgcgg
    10441 tgaatgtccg caaggatggc acgaaggaga tcatctgcgc caacaccacc agcggcgttt
    10501 ttgcggtgga cccgaagaag ggtgtgatca actggcagta caatcccggc ttcaatcagc
    10561 gcaacgtggg ctccggtgtc ctgaaggagg ggcgttattt ttgcaccttc ggcacaggag
    10621 gcggtgtgaa ggagtttgcc gcgattgatg tcactggcaa ggagccgaag cctgtgaact
    10681 tcgagttcag ccggggcctg ccttatgtgc cttcaccgtt ggtggtggga aatctgatgt
    10741 atctgctcgg ggatggcggc atcctcaagg cggtgaagtt cgacacgggc gaaatgcttt
    10801 atgatgagcg tgtcaacggc agcgcgggca gcagcaagtt tttcggcagt cctgtggcgg
    10861 cggatggcaa gattttttgc ggcagtcagc agggggattt gattgtgatc aaacagggcg
    10921 agaaattcga gcaactttcc gccagcaagc tcgacagccc catcagttcc agcccggcca
    10981 tcagtgacgg ccggctcttt gtacgcacgg aaaaaatgct ttggtgcatc ggtgccaagg
    11041 gcgcgccgtt gccttgatca gacctttttt ttccaaaaca cccagcactc cggcgggtgt
    11101 gccgccgggt tgatcgggcg gatttgctgc acttcaaagg agcggatggg cagttgatcc
    11161 gcccattggc agatggcctg ccgctcctcc cggccaccct cgtgacctgg atacaccgcc
    11221 agtgtcagga gaccgccagg tgccaggaga tgcaatgcgg catcaagcgc gggcagggtt
    11281 gtctgagcgc gcgtcatgat gcccttgtcc gagccgggca gccagccgag gttgaacatg
    11341 atgcctttga ttctgccatg gaagctgcac ggaatgcttt ggagcagtga ttcatgtccg
    11401 gcttgaatca ggctgtggcg gttcagttga attccgtgat cttcgaggcg gcggcgtgtt
    11461 gattccaggg cggcggcttg tacatccaaa gcaaaaacat ggcctttagg accgaccaga
    11521 tggctcaaaa aaagcgtgtc atgaccgttc cccgcagtcg catccacagc gagatcgcca
    11581 gcgcgcagat gtgattccaa gaaaatatgc gaccatcgga cagcggaggg aagtccttga
    11641 tgggttagtg gatgaagttc ctgcatcggc aataggaata ctgacatgct ccttcgaggg
    11701 caagcgtcgc tgactgcaaa ctttgatatt tcaaatttta aacctttgca aagcattggc
    11761 gcggaaccta aatttcagaa ttagcatatt tttgatgctg agggctttct ttggaagtcc
    11821 gagcgctgac gggaatgcct ctacaactct cgatccaagt gtgaacacgg tggcggattt
    11881 cacggcctga ccgggggcgg cagcttgtcc ggaaccagtg tgtccgcaaa agacaagcgg
    11941 cggcttgcca tctcctcaaa cacgcccaaa atgcgcctcc acttttttct gaaccgcttg
    12001 tgagccttca tcatcccacc gacgacctcc gtgtggcaga aatcctgccg ctgatccagc
    12061 ccgctctgct tatgcacgaa tatccgctgg gcgaggacga ggccgccttt atcgaggagt
    12121 cacgccgtgc cgccgaggac atcgtgaagc tgaagaacga ccgcctgctc gtggtgtgcg
    12181 ggccgtgctc gatccacgac atgaagtccg cgctcgaata ctccgagcgc atcgtcgagg
    12241 cgcgcgcgag gcacgccgcc gacctggaga tccagatgcg tgtttatttt gaaaagccgc
    12301 gcaccacggt gggctggaag ggcttcatca acgatccgca tctcaacgag agctacgaca
    12361 tcaacaacgg cctgcgcggc gcgcgcagcc tgctcatcga gctggcgaaa aagcgtgtcc
    12421 ccgccgccac cgagtttctc gatgtcatca cgccgcaata catcgccgac gtggtggcat
    12481 ggggggccat cggcgcgcgc accactgaga gccaggtgca ccgccagctc gcttcgggct
    12541 tgtccatgcc cgtgggtttc aaaaacggca ctgacggcgg catccagatc gctttggatg
    12601 ccattcaggc ggcggcggga gagcattgtt tcctttccgt gaccaaggac ggcgtggcgg
    12661 ccatcgtgaa gacccagggc aacgatgcct gccacgtcat tctgcgcggc ggcaaaggcg
    12721 gcacaaattt tgatgccgcc gccattcaaa ccgtggtgga ccagctcggt ccgcgcggtc
    12781 tgcccgccag cgtcatggtg gattgcagcc acggcaacag cctcaaagac taccgcaaac
    12841 agcctctcgt ggccgactcc ctggcggagc agctccgcca gggcagcaag gccatcacgg
    12901 gtgtcatgat cgaaagccac ctcgtggaag gccgccagga cgcgaagccc ggacaggaac
    12961 tggtgtacgg tcaaagcatc accgacgcct gcgtgaactg ggccaccacc gagaccatgc
    13021 tggagcagct cgcgcaagca gtccgggagc ggcggaaact tggctgattc ctgatccgca
    13081 tcatgttgaa cgcctccacc gcccgcgacg ccatccagcg catgtcgggc tgccgcatcc
    13141 tcgtcattgg cgacgtgatg cttgatcatt tcatctgggg cgcggtgcgg cgcatctcgc
    13201 cggaggcgcc ggtgccgatc gtcgaggtca cgaaggaaac gactttcccg ggtggcgcgg
    13261 ccaatgtggc gcgcaacctc tccgcgttca cgccgcacgc ctatttgatg ggccgggtgg
    13321 gcaaggacag cgcggcgggt gagttggcga aactcttgaa cgaggagggt gtgaacacca
    13381 gcccgatgct ggagagcgac acgcttccca ccattgccaa gacgcgcatc atcgcgcggc
    13441 agcagcatgt ggtgcgggtg gaccgtgaga tgatagaaaa actcagcgcg gaggagttga
    13501 aggaaatctc ccgcagggtt gaagcaatgc tgccggatct cgacggcctc atcatcgagg
    13561 actacggcaa gggtttcgtg acccaggcgt ttgtggacca ggttttgatg ctggcgaagg
    13621 cggcgggaaa gctcgtgacc gtggacccct cgccgcgcaa tccactgagc tggcgtgggg
    13681 ccacgctcgt caagccgaac cgcctggagg cttttgctgc cgcaggcatc caggacgcgc
    13741 gcacgccggg tccgccgctg gaggacgctc gtcttctcga agtcggggag cagcttctcg
    13801 atcaatgggc ggtcgaaaag gtgctcgtga cgctcggcga gcagggcatg atgcttttcc
    13861 gcagaaatgc cccgccgcat cacatcccca cgcgtgctcg cgaagtctat gatgtctccg
    13921 gcgcgggcga caccgccatc gccttcctca ccctcgcgct tgccgccggt cttagtgacg
    13981 aagccgccgc cgacatcgcc aaccacgcca gcggcattgt cgttggcaag ctcggcaccg
    14041 cgcgcgtcag tgtggacgag ctgttgcaag cctttgaaaa ttgatttccg tcatgtctga
    14101 tttttccacc atgttccaag gccatctcga cggccacacc gccactctgc aacgcctgca
    14161 ggaatgccgc gatgtcctcg ccgccatcgc tgaggcatgg gtcaccgctt tgaaggcggg
    14221 gaaaaaagtc atcttctttg gcaatggcgg cagcgctgcc gacgcgcagc atctcgccgc
    14281 cgagttggtg gtgcgttacc gagccaaccg ccgcgcgctg ccgtccatcg ccttgaccac
    14341 ggacacttcc atcctcaccg cgcacagcaa tgactacggc ttcgagaccg tctttgcgcg
    14401 ccaggttgag gcgctggcgc agcctgggga tgtggtggtg ggcatctcca cttccgggac
    14461 gagcaaaaat gttgtcgccg gtttgaaggc cgcgctcgag gcgggctgcg tggtggtcag
    14521 cttcaccggg caaaatggag ccgactgcgc cgcgcttgcc catcatgcct attgcgtgcc
    14581 cactcctgtc acggcctacg cgcaggaatg ccatctcatg gctgggcatg tgctctgcga
    14641 gtatgtcgag gccgcttttg cggccgtcta gtttgatgga tcgcaggcat tctgcctgca
    14701 cgaacaggcg ggacgcctgt tccccatgcg ttcgaagcat gtcagcttct tcccactccc
    14761 tgcggcgtgc tttgtccggg ctgccggttt ccattcttct gcgagtggcg cggctacttg
    14821 tcgggctcgg gttggcagcc atgctgaacc ggcatctggg gccgggaggt gcggggcaga
    14881 tcatgatcgg gctggcctgg gtggcgccct tgctttgcct ggctgagctg ggattcaacc
    14941 ggctcaccgt gcgcgaactg gccgcgcatc ctgacgcgga gcgtgaaatt ctcggcacga
    15001 cattttgcac gcgcatcatc gcgggcatcg cgctttgtgg agcggcgttc gggctttcgt
    15061 ttttgctgga gccttccgtg cgtgtgctgg tctgggtgta tgcgctgctg ctgccgacgc
    15121 acgcctcgtc cgaattgctg ctctggctgg agtccaaagg ccggctcgcc gaggcgtcat
    15181 gggcgcagtt tcgtggattc gccctcgcag gagtgctcat gatcgcgggt gtcatcgcca
    15241 aagcaccgcc ggtgtttttt gccgtgacct atgtggtgga aagctggtcg gccattgcca
    15301 tcctcgtttg gatgtatcat cgccagggcg gctccatgcg cgcttggcgc tggtctggcg
    15361 cgaggtccct gaaaatggtc cgcgaggcat ggccggaaat gctggcgcaa ctcgtgatgc
    15421 tcatgctttt ccgtgtggac acactcatgc tcggctggat gcgtggcgat gccgaggcgg
    15481 gcatttacag cttggcggtg cggctttccg aggtggtgta ttttatcgcg ccactgcttg
    15541 gaagcgcgtt tcttccgcgc ctggtggagt tgaggaaaaa tgatccggca aagtatgaga
    15601 cgcgcttttc ccagtatctt ggagtgaatc tcgcctttgc ttcgctgtgc ggtctggggc
    15661 tcacgctcgc cgcgccctgg ctggtgtggc tattgggcgg gaaggccttt gccggaagcg
    15721 cctcgatttt gatgattcat gcctggggag tgctggccta ctacttcggt gtggcgcgca
    15781 cgcaatacct cgccgccgag cacaggcttc atgcgaatct gcccgctgtg atcaccggcc
    15841 tcgtctgcaa tgtgctgctg aacctgtggt ggatccccca gtggtcgggg ctcggcgcgg
    15901 cgtgggccac gctagtttca tacacgcttg gctgggtggt catcacggca ttgtcccccg
    15961 gcctgcgtcc gctgctgcgc ttgcaatggc gcggtttggc cgggctgccc gcgctggtgc
    16021 gcgagtcctg gcgtaaacgc cgcgcttttt ggaatcctcg tcccgccggc atctcatgac
    16081 ccgcttcctc cgatctttgc tgagaaaata ctgcctcacg gcgaacacga tccgttatcg
    16141 cgtcgagtgg ccgcgcctgg ctgctgccat gcgtgtgtgt ggaccggtgg agacgctctt
    16201 tgatggcggc gcgggctcgg gtgagtttgc caggcggatg ctggatcatc acctttgccg
    16261 gaaggtcatc gctttggagc cggaaacttt tttattgctg caacaaaacc tcggtcatcg
    16321 cagcgaggcc aggctcatcg aaggctcgct gctggaggtg ccgctgccgg atgcctgtgt
    16381 ggacctggcg atgagcacgc aagtcatcga gcatatcgag gaccatgaaa gggctgtcgc
    16441 ggaaatggca cgcatcacgc gccctggagg ctggattttg atcaccgtgc cgcatccgcc
    16501 ggagcacttc ccgcagcccg ggcatgtgcg cgagggctac acgcggcaag acctggcgga
    16561 tcttctttcg ctctttggtt gtgcgcctgt gcatacagac tggttcctca cccggccaac
    16621 gacgacacgt ttcctcaaag cctgccgcct gcccttgaag ggcatgtatc tgcccgtggc
    16681 atgggcggac cgtgagacgg ctctgactcc tgagggaagg cgcttgcagg agccctttgg
    16741 cattctcatg ctgtttcaca aaaggtcatg accggcgcgc tccgccatgt gctttgggtg
    16801 gacccaaatg tcacacaaga cagcccttcc atgaaaggtc tgatgacagc cctgcccacg
    16861 ctcagagagc ttggctgggt gttcgagttc tggtgcttgc gctgcgatgc gccggagggt
    16921 ttcgtgaagg tgcgcaggct gcctgccgca ccctggctgg ggacattgga aaatgtctgg
    16981 ttcgtcctgg tggcaaacat ttgttatgtc tggcgttcgc ttgttttcgg gcggcccgcg
    17041 caggtggtgg gcagcacggg cgcttattgt ctgtttgccg atgtggtcca tgcgcacttc
    17101 gacagcgccg agtggttgcg cgtgcaggcg aggctgggtg gcatgtcctg gcgagatcgt
    17161 ctgcggcggc agatcacgcg gattggcgcg gctttggaat ggatgtcctg gcgctcgccc
    17221 tggacacgcg ctgtggtggc tgtctccgag ggcatggcgg ggcatttgcg gcaacggctt
    17281 ggaaatgcaa aatccgtgcg ggtgcttgcc aatccctgcg acacaggccg ttttcatcca
    17341 ggcgcgcgcg gacagtggcg cgaaaccatg cgggaacagt tgggctatga tgcccgcgcg
    17401 tgcgtctttc ttttcattgc caccggtcat ttgcagcgga aaggcttctg gtccgctctt
    17461 cgggtgctgc atcgtttgag gtcggaggga aaaaaagaga tccggtttct ggtggtcgga
    17521 ggctccgaca cgatgtggca ggctgagaaa aaacgcatcg ggaaagcagc gcctgatcac
    17581 gaggcatggg tgacgctggc tggcagcacg acaaatgtgg agcgctttta tgccgcagcg
    17641 gacgcgtttc tgctgccctc gcattttgaa acctacgccc tggtggcaca ggaggcgctg
    17701 gcctgcgggt tgccggtttt cgtcactgcc tactgggggc aggagatgta tctgcgccag
    17761 ggtgaaaacg gctggctgct gccctgggat gaggatggca tgaccgcggt tttggcggac
    17821 gcgctggacc agcgcccatg gcaggcaagg acggaacatt tcatggatgc acgggagtat
    17881 ggccgggcat ggtcgcaagt ttttcacgag cttttgaagt ccccgcatga gtgaaccctc
    17941 cagaggcgcg tcatctgtgc tggttatcct ggcagggatg gggctggcgc tttacatgct
    18001 cacggcggtg ctgctgtcag agggcaatct cgtgggccag ctttgccggt ttctgctggt
    18061 ggcgggtttt gtcatggggg tgctgctgcc gaggccggcc tttgttttct ggctgatcgg
    18121 ctgcggctac ctcgacctgc tcaagcgcct catggtcgtc tccgggcgca tgtttcatga
    18181 cgacttgttt tatgtgctcg gcgtggctcc ggccactttc gcgggcatcg cggcgggtgc
    18241 cctgctgcac gggctgttcg gcggtgtcaa gctgcgctca gcgcatctgg ggctgctggc
    18301 ggccgggtgt gtgatggtgc tgctcagcgc tttgatgtcc ctgcgcacca acggcatgtc
    18361 cctgcgcgcg ctcgggccgg atgtggcgaa cgccggcctg tatgccctgc tcatctttgt
    18421 ggtgcccgcg ctttatcagg atcgtgtgtc ggtgatgagg ctgctgcgtc tgctgctgct
    18481 cgcctggacg ccggtgatgc tctacggcgt ctatcagcag gtgaacggct tccaggagtt
    18541 cgagatagac tacctcaaga cgggcatgag catcgagatc aagcagctct tcagcgaccg
    18601 cgtgcgcgcc ttctcgacgc tgaactcacc cacagccctg ggcacgatcg cgggtggtct
    18661 gtgcgccatg gcgctcgtgc tcggatggcg gcctttgaac cgccggcaga ctgccgcttt
    18721 ggcttctccg gtggcctgga tgctggcgct ggtctatgcc gctggaatga tggcctccac
    18781 cgtgcgcacc tcgctgatga tacctgtggc gggactcatg gcggcatggg ccttccgtca
    18841 ccggggtgtg acttggatgc tgtatgcggt catggtgctc gctttcgcct tgctggtgct
    18901 ctcggcggac ttcattttga accgcatctc ggtctggaat gacctggcaa ccacgggtgc
    18961 cctgagccgg atgctgggag cggagatgac gagcatcggc accttcagtg accgtttgat
    19021 gggcttccgc aacatcatcc tgaacccaga cgcctacaca tggttcggtt acggctccga
    19081 gcgcggcagc gatccttcgg acccgctcta caaccatgac ctgatctcga acatgatcgt
    19141 ccgctatgga tgcgtgccgc tcgcggtgat gagtctggcg gggtttgtga tcgcccggca
    19201 tgtgcatcgt gccgtgcatc gcgtgcggga tgtggatgat caacgcctcg ccgccgcctg
    19261 tctgggcgtg gccttcgcgg tcgtggcggc gtcgttgttg agcggcaatg ttctggcgac
    19321 tttcccgaac aacgtcgtct tctggttgct cgtcagcatc acgctgatgc tttcgatgac
    19381 tggagtcaaa accagggcca aagccgcgcc ggatcagctt gagcctcaga gcgccgacgt
    19441 gccagcggct gtgatgcggg cgcacaggca gcacatcccg tcatgaagcc tcgtttgctg
    19501 ttgatcgggc atacctatgc cgcgctggag aaccggaaaa aaatccaggc gctggccgcg
    19561 catttcgaac tggtgtgtgt gacctcggag atcgagtccc gtgtcattct tgggcggccc
    19621 gcctctgact ttgacaatga cagcgacgcg cctggacgcg cctatgagct ggtgcggctg
    19681 ccaaggacat gcgtgaagga caccgcgttt gcgtatccgg ggcttggcgt ggtgatgcgt
    19741 cgaatgaagt ttgatgtgat cctggtcgag aacgagccct gggctgtgat ctgctggcag
    19801 gcgcgcttgc ttgccgggtg gctgcaatcc agcgcccgct ttgtggtctt cacctgggag
    19861 aatctggtga ggccgggcgt caaaggcagg attctctccg tcatctaccg cgcttcgacg
    19921 ctcaccacgg acggctacat cgcaggcaac aagcgggcgg cggagatcct ggtgaagcat
    19981 ggggctggaa aagaaaaggt gctggtggcg ccgcagcttg gggtggattt ggcaaatcat
    20041 cacccggtcg gggagcggga aaaaatgcga ctgcgtgtgg tgaacggcct gccgccggat
    20101 gccttcattg cgggctattg cggacggctc accgaagaga agggaatgga tgagttgctg
    20161 cgcgccggtg aaacgctggc gggagaggga atgcatctgg ccttgcttgg tgccggggtg
    20221 atgcgggagc ggctggagca gctcccagcc cggcatgaat ggctgcatct gctgccgccg
    20281 cggccgcact ttgagattcc tgatttcctc cgctgcctgg atgtttttgt cctggcaagc
    20341 aagccgctga tcaaccggga caactgctgg gaggaacagt tcggccatgt gctcatcgag
    20401 gcgatggcct gcggcgtggc gacattgggt tccagcagtg gggcgatacc ggaagtgctc
    20461 ggtgatgagc gggctgtctt tgaatgggga aacgcggagg ctttgaccaa gcgactgcgt
    20521 gtgatgaaga atgacgcggc tttgagaacc gaactggcgc gggcgcaacg cgggcgggtg
    20581 gagcgtcact acacacacga tgcggtggcg gagcactggg cgcggtttct gaccggcctt
    20641 catgcgggaa aggaaatgat gccatgaact atcgcgacat tttgtatcga gactactcgt
    20701 cgcatttcgg aggaatgaag gcgcatgatg ccagggtgca gtttgcgcaa tatgcggcga
    20761 cgtatcccgc gctgccggaa cgcggcgcgg cagtgctcga catcggctgc ggcaagggcg
    20821 agtggctggc gtggatggcg gagcagggat ttgattcgct gacgggcgtg gatggctccg
    20881 tgtctgatct ggagatggcg cgcgggcatg ggccggcaaa ggccgcgtgg gtgaacgagg
    20941 atgggatcgc gtttttggag aagaacccgc gcggctttgc tctcattcat gcaaaggatg
    21001 tgatagagca tctgaccaag gaggaattca tccgctttct tctggcggcg cgcgcggctc
    21061 tgaaagaggg cgggcggctg tggctgatga ctttcaacgc gcagagtccg atggcctcgg
    21121 ccacgcggta tggcgacttc acacatgaga cgggctacac gccggcgagc ctagcgcaat
    21181 gcctgcgcgc gtgcggtttt ggcacggtgg ctgtttccgg ccggcattac tgctctttga
    21241 gtcccggcgg gcgcgcgcgt tggatgctcg ggcgctgctt ttatgccctg gcacgaaggg
    21301 tcttgagact gcggcacggc gcgggagcag ccccggctgg agtggacctg cacacggcat
    21361 tgccggattt gtttgcggag gcacgggcat gagacgggtc ttgatttatt tccgcgagat
    21421 tctggtctcg ctgccgctgg ccttgatcaa aggctggaag tgtttcggct ggcgcgggag
    21481 ctggctggcg ttccaggatt tggttttcac acgcctgttt tttcccgtgc gggtgcctgg
    21541 ctttggcatg ctgaggcggc gcaatgaggc gcgcagtttg atggataatt tttgtctcgg
    21601 cgagctgagg tgcgaggagg tggaacaggt tttgcgcagt catcccgcgc cggtggtggt
    21661 ggacctcggc atcaatctcg gcatgagcgt gcgctggtgg tttcacctga atccgaaggc
    21721 gcgtgtcacc ggatttgaaa tgatgcagga ggcgctggac tacacggggg cgaggcttcg
    21781 tgatagccat cccgaggcgg attggacgcc ggtttgctgc gcggtgtccg ccgcggatgc
    21841 cgaggcgatg gaagtgtgtt ttgacgatgc cctggaggga acgacgagcg cggtccggtc
    21901 agcgggcacg caaaagcgga gcgtgcctgc gcgctgtctg gacagcgtgg tgcgggagga
    21961 tgatgtggtg cttttgaagt gcgacatcga aggcttcggc gggcatgctt tggaaggcgc
    22021 gcgcgcggtc ttgaaaagaa cgcgctatgt ggtgacggag acccacgggc cggaggagat
    22081 ggagcgcatg agccgggtgc tttttgagga gggttttgcg cccttcaagc tggcggcgcg
    22141 gtcgctttgg tggcgctcgt tggagttcaa gctttagctt gttcaatgaa ctcccgctgc
    22201 ggagtcaact cccgtgtcgc ctaaaggctt tactccaacg ggttcactcc aacgggttca
    22261 ctccaacctg gcaagcacac gctgatgaac ctcgtccaca ctccagcggt ccatgcaggc
    22321 catgggatga aacgtgtttt ggcagtgatt gacgggacgg gaaaagaggt cgcagggctg
    22381 gcaggcgagt gcggcatcgc gcaggaggtc gaatttttcc gaatgccaca ttgggtccca
    22441 gttcgggcgc gaaggtccgt tgaaaatggt gccgggaatg ccgagtgcgg aggcgaggtt
    22501 catggggccg gagttgttgc cgatgaaatg gcgcgcgcct gcgagatgcc ggatgaagtc
    22561 atgcaagctg cctggagtca cgcggcggat gcgcggggcg agcatttcct cgccggcttc
    22621 gttttgatcg aaccaaagta cgggatgcgt gtccgcgagc ttgttggcga gggcgatgaa
    22681 acgctccagc ggccagcgtt tgtaagctct gctggctccc gcatggatgg cgatggctac
    22741 atggccttcg cgtccgaagg cggagaggtc tggcgcgggg atggcatcgg gcagcgcggg
    22801 atgaccgagg cgcgcgagca tggcctgggc gatgcgccag ttttgccgcg cggcggagtc
    22861 ggtctcgatc aagggttcgc gatgatggag cagaaagcac agcttgcgct caggcacatg
    22921 cggtccgacg cagagcgcgg ctctgctgag gcgtgcgagg aggtgggcgg cgtttccctg
    22981 gtcattgccg aggaggcagg tgtccggctt caaggcgcgt gtgatgcgca gaaggcgggt
    23041 gagacgcgcg gggtttttcc aggaggagtt gaagtcttgt gtgttaaagg aaagcagtcg
    23101 cgcctccgga catgcgactt catacagcgg cgcggctgcc gggctggtca tgacggtgat
    23161 gcgccagtgc gggaagtgtg cggcaagctg ctggacaagc ggaaggaaga cgacgttgtc
    23221 gccaagctgg ttcactttga agatcagcag attcatcagg cggtgcatcg tttggcggaa
    23281 agggcgcggc tgcaagatgg agttcaatgt tggagttcaa gctttagctt gttcactggc
    23341 tttccgcagc ggaactcact cccgtgtcgc ctaaaggctt cactcctacg acttcacttc
    23401 aacggatcgc ctgagggcga tacaccagca tgaaaacggg aatcgctgaa ctgagactgc
    23461 ctttggcttc ggcggtgctt tgccttgcgg ctctggtggg cgcgctgcct tatgcggcgg
    23521 ggtatgggga cttccggctg acgatctggc aggagttggt gattcgctgg aaggatccga
    23581 cttggcagca tgggttcctg gcgccgttca tcgcgggttg gctggtgtgg cggatgcgcg
    23641 aggaaatcgc aacatggccg ctgcgcgcgc atggctccgg gctggtagtg atggccctgg
    23701 ccttgctgtc ctacttcgcg ggatacaagg cgcacaattt ttatctcggc gcgctgggca
    23761 tgcagggatt tgtgctggcg gtgatgctcg ggctttgggg ttgggcgcga acgcggcggc
    23821 tgggctttgc gtggctgatg ctgggcttca tgtggccgct ggtcttcatg gagcagggtg
    23881 tggcctatca actgcgcgtg ctgaccgtga gcggtgtgtc atgggtgctg aatcacaccg
    23941 gcgtggaaac cgtgcgccag ggcacggcgc tgctgtccgc tccggatgcg gcgcgcggcc
    24001 tgggagagct gttcagcctg aaggtggacg ggccatgcag cggcatgagg tcgctcttcg
    24061 cgctgctgat ggtctcggcg ttgtttggtt acttccggca ggggacgtgg ccgcgccgct
    24121 gggtgctctt tgcatgcagt gtgccgctgg cggtggtggc gaacatggcg cgcatctttc
    24181 ttttgctggg tggcaccgtg ttgttcgggc aggacttcgc ggtgggcaat gaggagcagg
    24241 aggtgtccac gtttcatttt gtgtcgggca tcgcggtcta tctggtggcg ctggcggggt
    24301 tgcagggggt ggcggctttg atggatagaa tttggaaagg cggagcagga tggacgcgga
    24361 agccgggacg ggtgagtgag cgtgccgcgc tggctgtgga gtcaaagagg cgtatggtct
    24421 ggcaaggcgc ggcgatgctg gcgatgacaa cggtggcgct cgccgtgtgc cgtctctcgc
    24481 ccgaactcag cgcgggcagc gaggccggag tggtgatgac gctgccggag cggacgagtc
    24541 ttttcatggg tcgaagtgag aagccggaca cagtggagaa gacgctgctg ccagcggaca
    24601 cgcagatcgt gaaaatgcgc tatgccacgc cgcgaccacc cgaggagcgg gatcgtgtgg
    24661 atgtcaccct ggtgctggcg ggggctgaaa ggcgcagcat tcaccgcccg gaggtctgcc
    24721 tcgacggcca gggctggacg atcctggagc agcgtgtggt gccggtggag ctgccgggcg
    24781 ggtgtgtgct ggaggtgaag gacctgctca tcgagcgcga gtggctggat gacgcgggcg
    24841 ggcggcggat gctgcgcgcg cattatgtgt attggtttgt cggctcggat gtgagcacgc
    24901 cgaacaactg gacgcgcgtc tggctgagca gttgggacaa tgtgctgcgc agtgtgaacc
    24961 accgctgggc ttacccgagt ctgacggcct gggtgactga aggctgggat ccggccctta
    25021 ccgggcagag acagcgcact tcggagcaga ctttggagtt gatcaaaaac ctgatccggg
    25081 aactggctcc ggggtttcag aaagggctga tgtaggcatg tgcggttttg tgggatgttt
    25141 tcatgcttca ggcggcggcc tggatgtgcc ggcgctgcgg cggatgctcg cggcggtgcg
    25201 gcatcgcgga ccggatgacg aggggctgca cgggttgtcg ttccatcgca gagaaagcct
    25261 gccgctggat caaatcagcg aggctcgcga gttggatgga ggcttcggct tcgcgcggct
    25321 tagcatccag gatttgtccg cagccgggca tcagccgatg acgagcgcgg atggaaaagt
    25381 ggtgctcgtc ttcaatggcg agatctacaa tgccggagaa catcgcgaga cgttgcgtgc
    25441 gcagggtgtg gagtttcgcg ggcactcgga cacggaggtg attctgcggc tgtatgaaaa
    25501 gcatggctgg cagggcatgc tcgaccgcct ggagggcatg tatgcgctgg ccatcgcgga
    25561 tgtgagggcg ggcgttgtga gagtggcgcg cgatcctttt ggcatcaagc cgctgtatta
    25621 ctggaatcgg aatggcacgc tgcttatcgc ctcggaaatg aaggctttct tggagcatcc
    25681 ggcttttcaa gcggaggtgg agacagagta tctggcggag cagttgcttt ttcgtcacac
    25741 cgcgtgggat cgcacactgc tgcgcggtgt gatgcaactg ccgccgggcc acactctgga
    25801 gtggcgccgg gatggcaccc tggcgcgcag gcgtttttgg agcctcccgg acgcggagga
    25861 tggcgtggtg gatttgtgtc aactgcgcgc gcgtctgcgc ggagcagtgg agagccagct
    25921 cgtgagcgat gtgccgttgg gaactcaatt gagcggcggc attgactcct cccttgtgac
    25981 gctggacgcg gcgcgcgcct cggcggagcg gctgcgcacg ttttccatcg tgttcgaaaa
    26041 tgacgcggtc agcgaggacg cttggatatc ccaggcggcg cgcagggcgg gagtacacag
    26101 ccaccggctg ctgttcaccg cgagcgactt cgcggcgggt tttgaaaagg cggtctggca
    26161 tctggatggt ccgccgggga atccgaacac gctggcgttg caattgctgg cgcgcgaggc
    26221 cgggcggcat gtgaaggtgc tgctgacggg cgagggctgt gacgagttgt tcgccgggta
    26281 taaccgcttt tattacacgg cgctgcagca ggcgtggccg ggggttttca aggcaggcgt
    26341 tcaatttgcc gggagtggga gagggaggtg gtggcggctt ttcggccggg cgggagagtc
    26401 actggagcat cagatggtga tctcctccgc ctatggggcc tcaggtttgg tggggagcct
    26461 gctgccgggc gtggacccgg agatggcgat cacggcccgt ctggatcatt tgagcggaat
    26521 tcctggcgcg ggtgtgcggc ggcaggtgcg atacgaggcg gagacttatc tgccggacct
    26581 gctgctgcgt caggacaaaa tgacgatggc gcatggtgtg gagtgccggg ttccgtttct
    26641 cgacaggtcg cttgccgccg cggcgagaag gctgcctttg gactggctgg cgaggccctg
    26701 cctgggggcc aatgcgcgga tgcgcggcac gaagcgggct gtgaagcaac tcgcgcagga
    26761 tgagtttggc cgcgcttttg cctaccggcg caagcgtgga ttcgatgtgc cgttggcgga
    26821 attttttctc cattcagagg tgcgcggcag agtggaggag gagtggctgc cggctttgcg
    26881 cgaccaggga tttttggact accgggctgt tgagaaagaa tggcgaagtc tgctcgaagg
    26941 gcgtggtaca ggagcggcgg ttgagacact ttgggtggcg atgggattga cagcttgggt
    27001 caaaatgatc aagaaaagtt aaacttccat aatttctgct tttcaaattc ctaagaagac
    27061 tctattttat ggaatatatg aagaaaattt ggctcgccat tttagctctc accatctcaa
    27121 caggtctgct ttccgcgcag gtcacggcca cggtcactgg cgtggagggt aatggctttg
    27181 caggcgttta tgatgtgaac ggcacgaatt attggtggat gtgcatcgag ccgaacggca
    27241 cgccgaacgc cgctttgggt gagtcgtaca cggcgaactg gttttggttt gaggacggct
    27301 gggaccagca gaacaacgag cgttttgact tcttcaatgt gcagaatccg gcctatcaaa
    27361 cgacggtgat cccgaagcag gtgacggtga tgacgtatgt gctggacact tatctgccct
    27421 gggacacgct ggctggcgcg tccggccgct tcatcgagca ggacagcgac ccgctgaatt
    27481 acgagaacaa cacggacttc tacaactcgc tcttcgtggt gcaaaatttc ctgtccgaga
    27541 tgtacgggaa gccgcaccag aatgacttca cggacctgag caatttcgtg aacaactggg
    27601 atggcgacgt gaccgcgact ggcatcgcca ggcaggcgct gtttcagtcc atcctggatg
    27661 acgtggaagg gaaggacggg gtgaatcatt tcgacactta cacggcgatc catggctact
    27721 cgactctcag cgtgcgtctg gacgaggctg atcctgacaa ctggcaggac gtgctggtga
    27781 tcacctcctt cgcccccgtg cccgagcctt ccggcgcgtt gctcatcgcc tgctgcggtg
    27841 tggcggtgat gctgcggcgg ttccgcaggc tggcttgagc agggcggtgt gcgggggggc
    27901 ttgcgcatga cgcggcttgg gttagcgtag atgcatgcaa gtcttcatca gtcatagcta
    27961 tcaagacgct gaactcgctc gcaagctggc cgggagtctg agagaaaacg gacttcgccc
    28021 ctggctcgcg gaggaagagg tctttccagg ggacaactgg ggagaggtca cgggcagggc
    28081 attgagtgaa tcggatgcca tggtggcttt ggtcacacct gtttcaggtg ccgcgccgca
    28141 ggtgcgccag gacatcgctt ttgccctcac ccgcaaagcc tacgcgaaca aagtcatacc
    28201 cttgatcgtg ggaaatcgcg acgatgtgcc tccgaacgcg ctgccctgga tcatgaaccg
    28261 ctacaagatg gtggagattc cttcgggcga tcaaatgccc atggctgcgg agcaaatcgt
    28321 cggcgcgttg cgcgggcatg aatccatgtt ggagacggcg gcatgagggg gccggtggag
    28381 gtttttctct cgcattcatc ggatgatgcg gagacggcca ttgagattgc gcgcgtgctg
    28441 ggagagcacg ggatttccgc ctggtatgcg ccgcgtgaga ttgtcggctc gcagaagtgg
    28501 caggatgaga ttggtgcggc gttgcgccgt tgtgactggt ttgtgctgct gctgagtccc
    28561 ggggcggtgc gttccatgtg ggtgcgacgt gagctgcaat atgctttgat acagagccgg
    28621 tatgatggac gcatcactcc tgtcttgatc tcgccttgtg atccagacaa cctttcctgg
    28681 gtgctgcaaa catttcagca agtggacctc accagacgaa ctgaaggggc atattccaac
    28741 ctgctgcggg tgtggggaaa ggagtttggt ccggtagctt gaatcaaagg ttggtggaat
    28801 gcaccatgtc tctcgaagac agactctacc cgctgctgaa gctgtatgat gcggcgccgc
    28861 aggctgtgaa atcgctggcc gggcgggttt atcgcgcgat tccgcccggc ttgcgacatg
    28921 gcagcgccta tccacgtttt ctggacgagg cacgggaggt ggagtcctgg gatggggaga
    28981 cggttgagag gtatcaaatc cgggcgttgc gcgagtcgct aattgccgcc tccaaagctc
    29041 cgttttatgc gcggcgtttc gcggagagcg gagtccgtcc ggagaagttc gagtctttgg
    29101 aacaactggc ggactatccg ctgctgacca agcaggattt gatctcgcag cgcgagcaga
    29161 tgctgaaccc ggaattcggg ccggagcatg ggctgcacat caccacgggc gggtcgagcg
    29221 gcgtgccggt gggatttgat ctgcacaaag gggtgagccg tccgaaggag caggcttacc
    29281 tggaggcgca gtgggcgcgc cgcggctatc gtgccggcga ccgcgtggcg gtgatccggg
    29341 gcggggtgac atcgagccag gcgagcggcg gcatcagcca tcatgacgcg acgcgggact
    29401 ggctgatctt gtcctcgtat catctgacgc cggagcgcct gccggagtat gtggaggagt
    29461 tgaaccggtt ccagccgcgc catttgcatg cctatccgag ctcggcgctc atgctggcgc
    29521 gtggcatgga gcaggcgggg ctgaggctgg atttccccct gacatccctg ctttgcggtt
    29581 cggagaagct cactgccggg tcgcagtgtg agctggagca gtttttcggg acgcgtgtgt
    29641 ttcactggta tgggcacagc gagcgcgtgg tgctggcggc gcagggcagg gaatcgaaca
    29701 tgctgcattt ctggccgacc tacggctttg tggaattcgg cgagccggat gcggagggga
    29761 actgcgagat catcggcacg tcctttcaca atcatgtcat gccgctcatc cgctatcgca
    29821 cgggggattt ttgcaagccg acgcttgaag cgcgtgagtt tggctggcct gcggtcgagg
    29881 ctgtggtcgg gcgcgattat gagttccttg tcagcggcac cgggcggcgc atttcgctca
    29941 cggccatcaa catgcacgac cgtctttttg acggcctgct ggcggtgcag tttttccagg
    30001 agcagccggg cgcggtggag tgccggttgc agcctggtcc acactggcag tcagcccggc
    30061 tggaggaaat ccgcgccgga ttgctggcga aacttggtga tgacttcacg ctcactttgc
    30121 gtgaggtggg ggaggtcgag aagactcccg ccggcaagca tcggtggttg gtgacgactt
    30181 tgaaacagtg atccggtcag atatgagaca actcgcgcaa tatcaggatg gccgcctcga
    30241 actccaggag gtgcccgcgc cagtgccgcc gccggggggg attctggtgc ggacgacgtg
    30301 ttcggtgatc tcgccgggga cggagcggat gaaggtggag caggcgcgca tgtcgctgct
    30361 gcaaaaggcg aaggcgcgac cggaccaggt gaagaaagtg atcgacacgg cgaagacgct
    30421 gggctggaag gcggcgctgg caaaggtgcg caaccggctc gaatcaccga cgccgctcgg
    30481 ctattcggcg gcgggtgtgg tggtggcggt ggatgagttg aacacgcgtt ttcgtgtcgg
    30541 ggatcgtgtc gcttgtggtg gagcggagtg cgcgtttcat gcggagatga tcgctgtgcc
    30601 ggacctgctc gccgcgccgg tgccggaagg tgtggaggac tggcaggcgg cctgcatgac
    30661 tctggcctcg atctccatgg aggcggtgcg gcaaagcggc gcgcgtctcg gcgagcgtgt
    30721 gcttgtcctg ggccaaggtc tggttggctt gctggcgacg agcctgctca aagcttcggg
    30781 ggcgcgtgtg atgggggtag atttggtggc tgagcgactt cagacttcgc tggccatggg
    30841 ggcggagcgc gtggcgaatc ctttgcagtc gagtctcgct gacgaggtgc gtgaatggac
    30901 cgccgggcag ggtgtggatg cggtgctgct atgtgctggc ggcaagggac gcgacgcggc
    30961 ggacacggcc atcgaatgcc tgcgcgaccg cggtgtgatg gtcatcgtgg gcatttatga
    31021 cgcggagctt tcctggaaga ccgcctacat gaaggacatc caggtgcgct actcgcgcag
    31081 ttacggccct ggacgctacg atcctcagta tgagtggggc gggcaggact atcccatcgg
    31141 tcatgtgcgc tggacggaga accggaactt cgaggcgtgc ctggaactga tgcgcacggg
    31201 gcagcttgat ctggggcctg tgatgacgag gcgggtggcg tttgaggatg tggtgggagt
    31261 ctatgacgcg ctggagtgcg aggtgggcgt ggtggtggag tatgcgggta ggacaggcat
    31321 tctgcctgtc gcgggtgaat cgggcatttt gcccgatgtt gggaagccgg gcagaatgcc
    31381 cggttcacat gagtcaggcg ggacgcccga cctacgcgcg ttcatcccat tcgatccgac
    31441 aaagaaaaca gagaaggccc ggcgcaatct tccacactgg gagcaggaag gagtgacaca
    31501 gtttgtgacc ttccggcagg cggatgccat ctctcaagaa aagctcgcgg agtggcgtgc
    31561 cgagcgcgaa gtgtggctgc gggagcatcc gcagccctgg gacttcgaga cggaggaact
    31621 gcatcaacgc ctttttggcg accgtgtcga gcaatggctg gatcaaggcg cgggcagttg
    31681 catcctggca cgcgaggacg cggcgcgggt ggtggagaaa gcgatgcgcc attttgacgg
    31741 ggagcgctac attctggacg cctatgtcat catgcccaac catgtgcatg tgctgctgaa
    31801 acctttggct gggcactcgc tttccgagat catgcattcc tggaaaagtt tcacttcacg
    31861 ggagatcaag aagctctacg gcttgggaga agaggcgttt tggcaggcgg agcggtttga
    31921 tcatgcactg agaagcgagg cggcgtttga ggcgaagcgg gagtatattc ggaggaatcc
    31981 ggtcaaggcc agactcaatg gcgggtttgt ggttgggtgc ggcattgggg tggcgagtag
    32041 ggcaggcatc ttgcctgtcg cgggggcctc gggcattttg cccgatgacg gggaggcggg
    32101 caggatgccc ggttcacacg agtcaggcag aatgcctgac ctacatgtca tcggtgcggg
    32161 caactttgcg cgcacgatgc tgctgccgca tttgaaaggg cgcatcggtt ttggcacggt
    32221 ggtgaattcg acggggcttt ccgcgcggca tgtgagggag aagtttggct ttgccgcggc
    32281 ggagacggat gcggtcgcaa ttctggccca agcgggcggc gcggtgctca tcagtacgcg
    32341 gcatcacctc cacgctcctt tggtcatgaa ggcgctccac tcaaacaagc atgtttttgt
    32401 ggaaaagccg ctctgcctca cgcgcgagga actcgcggaa ctggacatga tgatgcaggg
    32461 cacgcaaggc agtgtcatga ccggtttcaa ccgccgcttt gcaccggcga cgctggcgat
    32521 gatggagaca ctgcaaaacg tctccgggcc gaaaaccctg gccttccatg tgaacgcggg
    32581 tgttttggcg ccggatcatt ggtatgccaa cgcggccgaa agcggcgggc gtgtgctggg
    32641 tgaggcgtgc cacttttttg acttcgcctg ccatctgctg gggcgtccgg tgaaagtgac
    32701 ggcgcagaca gttggaaggc ccaaggcgcc ggactcggtg acggcgcagg tcgagtttgc
    32761 cgatgggtca tccgcccaga tcatctactc ggcggagggt gattccgcgt ttccgaaaga
    32821 gtctttgcgc gtgttcgctg gcgggctggt gatggagtgc gagaacttca tgcgtctcgt
    32881 catttaccgg aaccgcaagc agacggtgag gaaattttca tcgaaaggcc acgcggagga
    32941 gatggcggct tggctggcct ttctccaggg caaagccgcg catccgctgc cctacgagca
    33001 gtcacgccag agcatgaggc tcacgtttgc cgcgctggac tcgatccgcg aggcgcggtc
    33061 cattcccgtc tgagacaacc atgagcactg gaggccgcct cttttggtat gcgcatcgtc
    33121 tgcgggcgat gagcgccgca gagatcatgc accggatggc ggagcgcgcg cgccggttca
    33181 cattgggcgt atgcctgaaa agggccgccg gcatccagcc cggcccggtc aatgcccgcg
    33241 cgcctttcct gcccgatccc gccgccgctc cggaaggcat gcgcgaggcg cttgccgcag
    33301 aggccgcagc gttgcagcag ggcaggtgga agcttttcgg ctggcatgag gcggaggtgg
    33361 gcgcgcctcc gtgctggcac cgggacgcgc gctgcggtgt cgtcatcgag ccaaagaccg
    33421 ccgcgcaccg gctcgaccac cgcaacctgc cggacggtgc ggatgcgcgc acgatctggg
    33481 agatcaaccg ctgggcggag atgacgcgcc tggccatgca tggctggctg aacgcggaca
    33541 aggcggccat ccgcgcggcg cagctttggc tggaggactg gctggaccac aatccgacgg
    33601 gcatgggcat caactggacc agccctctgg aggcggcgct gcggctgctg aatttctgct
    33661 ggttcgacgc cctggcccgc gcggcaggcg cggacacggc ggtgcaggac aaactggcgc
    33721 ggcgcatcgt gccaatccat gcctgctggg tctggcggca ccgttcgcac gggtcttcgg
    33781 cgaacaacca tttgttaggc gagctggccg cgctggtcgt ggccttgaag cgctggccgg
    33841 gcatggaaaa gcatgtcctt cctgtggagc aggtctggga catgctcgga gcggaggtgc
    33901 tggcgcagtt cgcggaggac ggcggctcgc gcgagcaggc gctgcattat catctgttcg
    33961 cctttgacct ggcctggcag gcggcgcgcg ccgtggggtg ccgggcaggg gaggttcatg
    34021 accggcttgc ggaagccgcg gactatttcc gctgcctcgc gcatcctgat gagccgtggg
    34081 attttggcga caatgacgat gcccgggtgg tgccggtcgc gcgctcgcgt gagcgcgctg
    34141 ttgcggagtg gagggcatgg ttgggcggag agccggggga cctgcgcttt tggctggggc
    34201 ggagcccgag cagtccggcc agggtggagg aggcgggctg gcgctgcttt ccggaaagcg
    34261 gcatggccgt gggccgcatg ggcggttgga tggcgcggct ggacgcctcc ccgctcggct
    34321 ttggcagcct ggcagcgcat ggtcactgcg acgtgctgca tctctcggtg tgggatggcg
    34381 cgcatgcgct gctgattgat cctggcacgg gcggttatca tggttttgcc ggactgcgcg
    34441 cggagctggc ggcgtgggag gcgcacaacg ggccgctgcc ggccggcggc taccgcactc
    34501 cgcgccgggt tggcgctttt ttgcagacag cgcatcatcc tcaacccttg ctggcggcgg
    34561 agggcgggcg ggaggtggcg cgtttcgcgc acgaggggcg catcatgcag cgcacggtgg
    34621 tcatgaacgc ggatgggatc gaaattcagg atgtcgaggc agccggcaga ccgctcgtgg
    34681 tgcgctggcg tctggctcct ggcatggcgg cgaaaatgct ggcgaatcag gggcgcgcat
    34741 gtcttgaact ctccctcaaa ggcgtccttt gggtcatgga tcccggtgct gacgaggcga
    34801 atgtgaggct ggccgagaag cgcgcctcgc ccttttacgc ggagattgcc agcagcgtcg
    34861 aggtggaggt gaggtgcgcc ggaggcagtt tgacgacccg tcttcgacga ggctgaccgg
    34921 gcatcagcaa gcgaggcagg agcacgccgc aggcgtgctt ttttgcgccc ggagcgccgg
    34981 cttgcctgga aaaaagaagc gccgcccggc ctggatgggc gggcggcgcg acgtatggcg
    35041 tcagggacgc cggggatcag ggcttggccg tcgggcagca ggaggcggct tttttgccgc
    35101 cgctgtcaca gcaagtggcg cagtcggact tcttggaggc gcaggaggcg agcaccaggg
    35161 aggccgagac cagggcaagg gtaagaaggc gtgtcattat gagagcaggt tgtctgcgtg
    35221 gacgcggacc ttcttacttg ccgtaatgca ccggagccgg cggcgggggc gggggagcct
    35281 tggaggcgca ggagaccagg gtggagatgg cggccatggc gagcagggcg aggacgatgt
    35341 gtttcatagg ctgttgttgt tcttgtgttt attgctggat tcgaggaatc gaatgccgat
    35401 gcgccgcgag agtaccggca atttgatttt ttgcaatgct tcaaatgccg atgttttgcg
    35461 gacttttcgc aagtctgaaa aggcttaccg ggccagattg agatccccgg tgaagggagc
    35521 gcgggtgcgg tcgagctcgg tggcgagggc ggcgcgcaga cgctcgagtt cgggacggtg
    35581 ggcggcgctt gcggccaggt tcgcgagctc ctcggggtcg tctttgagat cgtagagttc
    35641 ctcgatttct ccggcgcgca ggtagcggat gtatttccag cggccgtcgt tgagggcgac
    35701 ataccagggc actccgccgt gctcggcctc ggcgggggtg gcgcgcagga ctttggccac
    35761 ttcgcttccg taatatttgc cggtgtgctc gtagaggcag ggggtgatgg cggcgggcgc
    35821 gtccggggcg gtgagcaggg gggtgaggtc gcgcccgtgc atctcccact cgggttcgag
    35881 gccggcgaag ctgaagaagg tgcgcacgag gtcggggctg ttcacgggct gcgcgcagac
    35941 tttgccttgg gccaggcggc cgggcatgga gatgatgaag ggggagcgca cggcggcgtc
    36001 gtagggggcg agtttgatgc gcaggccgtg ctcgccgagg gcgaagccct ggtcggcggt
    36061 gaagacgacg agggtgttct cgatctggcc gctttccttg agcgcggtga tgaggcggcc
    36121 cacgccctcg tcgagggctt cgacgcagtc gttgctctgc tggacgaact tctgaaatgt
    36181 ggtgccgccc ttgcctttgg cgccgacgat tttgccgtcg ggtcccattt tccaggcctg
    36241 ggtcttctcc agccaggcgg gtttgccggg gcggctgccg atgatgtcgg cgggcacgcg
    36301 tacttcgtcg tttttgtgtt tgcctttgtg gcgctcggcg ggggtggtgg ggccgtggat
    36361 ggcgccgtag cagagccaga ggtaccaggg tttgtcggcg gggcggtttt tgccctggat
    36421 gaaatcgcag gcccagtcgg tgtagttgtc ggtggagtag ccttcgagga tgccgagatc
    36481 acgcccgttt tcggtgaagc gctggccttc ataataggcg ccggcgttgc cggtgtggag
    36541 ggggcggttc cagacctttt ggtagtccca gtcgcggccc cagccggtgt cggtgccggt
    36601 gtgccatttg ccgatttgcg cggtgtggta gccggctttg cggaaggtgg cgggccagaa
    36661 gcggcattga tccgggtcgt aggcgctgcc ggggtagggg ccttccatgc gcatggtctc
    36721 gatgctgtgg gggtgcaggc cggtgagcag gctggcgcgg gagggcatgc accaggagcc
    36781 catgtaggcg tgggtgaagc ggatgccgga gcgcgcgagg gcgtcgaggt tcggcgtgct
    36841 gactccaggc agggcctccg ggtaggcgga gacggttttg taggattggt cgtcgctgaa
    36901 gatgaaaagg atgttcggct tggcggcggc ggcggcgggg agcaggagga tgaggctgag
    36961 gaggcggagg gcgtgcatgg cggggtggta acgcggcaga tggcggggga attgcagtct
    37021 gacttggcgt ggtccgggga tgtgattttg cttttgccgg gcggcgggca tgccggaggg
    37081 ggcgcgctac cttgagggcg gcgtgcaaaa ggattttgct tttgccgggc ggcgggcatg
    37141 ccggaggggt gtgatgcgta aactgttctg gttcaatgca aaatcgaact ctgaaattgc
    37201 ccggcaggtg tcccgggagg aaaaacaaga gcgtcggatc gtggcaaatg agtgtcaggc
    37261 gggtgaatcg gagcctcgga tttgggtgca tggcatcgcc tttccgcgat ctgcgggcgt
    37321 tttttggcgg gggggcaggc gggggctgga tccggcggtc g
//
LOCUS       DAOMYJ010000027        36926 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Prosthecobacter sp. isolate AMR_MDS_5629
            NODE_428_length_36926_cov_23.846546, whole genome shotgun sequence.
ACCESSION   DAOMYJ010000027 DAOMYJ010000000
VERSION     DAOMYJ010000027.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867850
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prosthecobacter sp. (wastewater metagenome)
  ORGANISM  Prosthecobacter sp.
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Verrucomicrobiaceae; Prosthecobacter.
REFERENCE   1  (bases 1 to 36926)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 36926)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.30
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:34:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,742
            CDSs (total)                      :: 4,684
            Genes (coding)                    :: 4,676
            CDSs (with protein)               :: 4,676
            Genes (RNA)                       :: 58
            tRNAs                             :: 55
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36926
                     /organism="Prosthecobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_428_length_36926_cov_23.846546"
                     /isolate="AMR_MDS_5629"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1965333"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(127..1440)
                     /locus_tag="PLP58_04700"
     CDS             complement(127..1440)
                     /locus_tag="PLP58_04700"
                     /inference="COORDINATES: protein motif:HMM:NF013741.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter associated domain-containing
                     protein"
                     /protein_id="HRJ07844.1"
                     /translation="MALDPRRILLTLAAVTLAAAILYLSLLALLAVISAVETAIHSAR
                     DIEPRLEAAGPGMAQKLRSIAANPFAHLHRALLLSATLNLALAALGLHLVSGPLRAAG
                     WNPWAATALIFCATVLVGDVLPKFLAARAPTSVLQGGLRLLVPLQPVLDPFAAWADKI
                     TDLLLKRVIPSRVKLNPKITRNEFETLVEMRQEQGLLGADEAEMLREALDIETLTVRD
                     CMVPRVDLTLMAVDDKPEKIAAALEQSSGRFVVFHGETPDMVEGVLDTEAWKLAGRPD
                     WRGMLFSPSFVPETLPVLDALDQHLKRTAMPVLIADEYGGLEGMVGQEEIADWLLHEA
                     APWLGEASEIRHLGGGRYIVDGGTRIDDVNRELRAAIPDEGVDTIGGFVFNHLGHMPK
                     AGERARLDGITLKVRRVVRARIIEVELRLDEARRAELQSVEEAGA"
     gene            complement(1466..2590)
                     /gene="rlmN"
                     /locus_tag="PLP58_04705"
     CDS             complement(1466..2590)
                     /gene="rlmN"
                     /locus_tag="PLP58_04705"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009957958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016433 - rRNA (adenine) methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HRJ07845.1"
                     /translation="MSHPQPPASLLGITPPELAAAFTEMGEPAFRARQVLEWTFQKRA
                     AGIEGMSSLPKALRAALAERFVTRTMSIARVTGSKDTTRKFLLRLSDGRYVETVLIPA
                     NPALYGGKSDRRTLCVSSQVGCAYDCKFCASGLAGFTRNLTAAEIVEQIVQVEQHTGE
                     RVDNLVFMGMGEPLANYSHVTKAIEILNAEWGVGIGARHMTVSTSGLAPQIRRLADFP
                     LQIRLAISLHGATDEVRDRIMPVNRKHPLGELFAALEVWHEKRKQHLTFEFILIENVN
                     DSLEQARALARLARPLEAKVNLIPYNTVEGLPWTRPSEECQDAFLGALLKAGVKATLR
                     REKGHDIAAACGQLRLKQETAEGIIESPVPARRVTIGAGA"
     gene            2722..3945
                     /gene="lpxK"
                     /locus_tag="PLP58_04710"
     CDS             2722..3945
                     /gene="lpxK"
                     /locus_tag="PLP58_04710"
                     /EC_number="2.7.1.130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009959153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009029 - tetraacyldisaccharide 4'-kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetraacyldisaccharide 4'-kinase"
                     /protein_id="HRJ07846.1"
                     /translation="MKDTLEDLEQYLIEVIIHHKRGIRPTLLRAAFRLLSGIYSGAVR
                     LRLFLYRERYIHDHHLGVPVISIGNITVGGTGKTPVVELFAKALHERGRRVAILSRGY
                     KSKRQRKIPLGWRIAAKLGFARKPREPLPRVVSDGERVLLDSYVAGDEPYMLAQNCKG
                     VPVVVDRNRVKAGAHAIRKFGADVLLLDDGLQYLKLKHRHDIVLVDKTAPFGTGHMLP
                     RGTLREPPASLRRASYIFLTKSDGDSGEVIEQIRRHNRVAEIIECRHRPVHFEDIHTG
                     ERLPLDAMSRKYVGALSGIAVPESFENGLRKLGARVQWVKRFTDHHRFQEKEITQFIE
                     RCEKAGAHAILTTEKDFVRFPKLPPGDLPIYFLRVEIEITRGRDIFDKLIRLIAEPRH
                     VTQGLVSADLVEATS"
     gene            3942..4766
                     /gene="fdhD"
                     /locus_tag="PLP58_04715"
     CDS             3942..4766
                     /gene="fdhD"
                     /locus_tag="PLP58_04715"
                     /inference="COORDINATES: protein motif:HMM:NF001943.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory
                     sulfurtransferase FdhD"
                     /protein_id="HRJ07847.1"
                     /translation="MTDAENAPAAHALVRRVTVGGGGEERADVTAREEPLEIRVEGRS
                     VAVVMRTPGHDEELAAGFLVSEGVIKTARDILEISQCPSMGNTHGNIVDVLLGGAVVN
                     WESLTRHVFSASSCGLCGKTSIESVFQSFPAVTADARVEASVIASLPEKLRAAQETFA
                     ATGGLHASGIFNLEGNLLVLREDVGRHNALDKVLGFALQRNLLPLDRHILLVSGRVSF
                     EIMQKALAAGVPVVAAISAPSSLAVDFAREGGQTLIGFLRGGTMNVYTHPARILNS"
     gene            4785..5117
                     /locus_tag="PLP58_04720"
     CDS             4785..5117
                     /locus_tag="PLP58_04720"
                     /inference="COORDINATES: protein motif:HMM:NF023376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3467 domain-containing protein"
                     /protein_id="HRJ07848.1"
                     /translation="MSSNVKIVGAEAPPAPRAQPAPAAAYSNVSRVFATPNEVVLDFA
                     MNLNAFGPLIEEDARIVSRVATSYDGAKRLWVHLTQTLQAYEQKYGVIELDLSKRAKT
                     ADAGAVEK"
     gene            5187..5909
                     /locus_tag="PLP58_04725"
     CDS             5187..5909
                     /locus_tag="PLP58_04725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009961171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HRJ07849.1"
                     /translation="MKFCIFGDIHANLEALQSVLWDAHEQGCASHVCLGDIVGYAANP
                     AECLETVREMNCPTVRGNHDEGAASTSNLEELNPLAQAALLWTREQLSEEQRQWLRDL
                     KLVRQVRDFTIVHATLDSPGSWGYVTNRFDAMASFSYQFTQVCFYGHTHVPRIFEKDD
                     SVRAARGNEVTLQRGVKYFVNVGSVGQPRDGDWRASYAIYDAQAQTIAIRRVEYDIQT
                     AQDKIRAAGLPSLLAERLALGK"
     gene            5909..6916
                     /locus_tag="PLP58_04730"
     CDS             5909..6916
                     /locus_tag="PLP58_04730"
                     /inference="COORDINATES: protein motif:HMM:NF013257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HRJ07850.1"
                     /translation="MNTRKLPDLADFRSLLVVKPSSLGDIVHTLPAVHAIKNAHPHLR
                     VRWLANPEWAPLIEGSSVVDEVVPFPRKSFRGLLGGMRFLNWISAQWRRRPHPEPEIV
                     LDFQGLLRSGLVARLSGSRPVVGLSDSREGARFFHDHVIAVNAGAHALERYLEVPSAF
                     GIPLEKPRTPLPEGTMPAGWPEREDAVVVHPWSRGKGKSLEPAVLEALCAALAPSPVV
                     LVGVRNGVSVPRGEHITDLSARTTLPELVWLLRRARGVVSVDSGPMHIAAAVNDRTLG
                     IHTWSDPRQVGPHNPRAWVWKDGRIAHRCDFASAECASTRSPNTEDARALAEFVKKLA
                     S"
     gene            6988..7854
                     /locus_tag="PLP58_04735"
     CDS             6988..7854
                     /locus_tag="PLP58_04735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009958247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HRJ07851.1"
                     /translation="MKPVFEKVPRRDWESFHCEVIEGPDYGTRWHFHPEYQLTLALRS
                     SGHRVVGDNIAPLTDGDMVLLGSNLPHIWHQDAGKGGVEAVVIRFEENFLGRDFMNMP
                     EAEGVKRLLRRAARGLEVRGQTRKEVAQRLRHLAEAGGLTRVVDLLAILDILACARDL
                     RPLASAGYEPTLHATDQGRMERVCDFIHQHLTEDIDRGQLARLAHLSEGAFSRFFRSR
                     TGKTVPEYINEVRIGRACRLIAEDQHNITDIALDCGYRNLANFNRRFREVVGRTPRDY
                     RAQMRTFGGGQA"
     gene            7899..8372
                     /gene="ispF"
                     /locus_tag="PLP58_04740"
     CDS             7899..8372
                     /gene="ispF"
                     /locus_tag="PLP58_04740"
                     /EC_number="4.6.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007415552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008685 - 2-C-methyl-D-erythritol
                     2,4-cyclodiphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 2,4-cyclodiphosphate
                     synthase"
                     /protein_id="HRJ07852.1"
                     /translation="MIRTGIGYDVHCFETGRPLVLGGVVIPHSMGLKGHSDADVLCHA
                     IADAVLGAMGLPDIGFYFPPSDMSIEGISSLRILEKCAALAKEHGFAIHNVDSTLVAE
                     APKVMKHAPSMKENIAAALGITPGQVGVKATTNERMGFVGREEGIAALAVACVEG"
     gene            complement(8382..9461)
                     /locus_tag="PLP58_04745"
     CDS             complement(8382..9461)
                     /locus_tag="PLP58_04745"
                     /inference="COORDINATES: protein motif:HMM:NF014336.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S1/P1 nuclease"
                     /protein_id="HRJ07853.1"
                     /translation="MRFPSFLIGVFMVVQPARAWWDGGHKAIALIAYDHLSESERAWV
                     MTRLEAHPTREELFELPLREEFASAAPDADTRARWFFAQAAIWSDLIRRDGGYPNAKE
                     INAAHHHSTWHYTDLAVFPDNAARAALAAKAAPPPMDWQPGMTEPEEGFNSIQTLRRL
                     LHELADPARDHGGQGVNLCWLFHCLGDTHQPCHCAQIFIPEKLPDGCEGANRIMILGL
                     KSAQPVLTSDVLHAFWDSLWNDEKNGLADVSARLAPLKADAALWSKGAEAARELDPLE
                     WLKEGHALAESHVYSPALLRRLASTSAQPNTRSSRGGNVMMVSMTTLDRDAYVREARF
                     VSRQQMVTAGLRLAAVLKDVIARSH"
     gene            9560..10024
                     /locus_tag="PLP58_04750"
     CDS             9560..10024
                     /locus_tag="PLP58_04750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009963883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxycytidylate deaminase"
                     /protein_id="HRJ07854.1"
                     /translation="MSDTDPKRTRLTMPEYAMALAHVASLRSEDPYRKVGAVAIDFDN
                     RVIGTAYNGLAPGYNASPEFWHDRDDRRKYMLHAEVNLCSLFTRGNVKLVACTTKPCT
                     SCMQMLCAYGVREVYYRDDYPESEADAIAQRYSIQLVRLTDYPRIIAGFEAG"
     gene            complement(10253..10753)
                     /locus_tag="PLP58_04755"
     CDS             complement(10253..10753)
                     /locus_tag="PLP58_04755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ07855.1"
                     /translation="MSLDDIKTSSFRRKTSHDMEEQMTMRLEGVSASEWDWVRRLIAD
                     VQEQERHEHLVALWAQWRIAVRFFRQAEFILMRQKQPGAVDFKFHRACLTGLISIGEF
                     LLLHIAESGDREELSRLGFSGENAEAALATLRSNWDEWHGESSPDRIRSIQQKLLELN
                     GEAQAH"
     gene            complement(10743..10955)
                     /locus_tag="PLP58_04760"
     CDS             complement(10743..10955)
                     /locus_tag="PLP58_04760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ07856.1"
                     /translation="MLSRSELARLIDGPALDREIRRLGKMGGGWIHAASVPAVLALAK
                     GFDVVFEELKMAPELAKGMASCIDVT"
     gene            complement(11186..12436)
                     /locus_tag="PLP58_04765"
     CDS             complement(11186..12436)
                     /locus_tag="PLP58_04765"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009959230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide pyruvyl transferase family
                     protein"
                     /protein_id="HRJ07857.1"
                     /translation="MQRRAFLTSALAAVLSPIAAREGRAARIVLRSSWQTVNIGDIAH
                     TPGMLALLEKHLPGVEVRLWPSKVDNGVDELLMARFPKLKILKSQAEIQTAFEECDFL
                     LHGSGPSLVAQRDVIRWSEATGKPYGIGGITFSTRGSTSTRDAADEAVQATLRVFNGA
                     RFVFFRDSVSLALAKSLGCTCPVMEFGPDGAFATDLREEEKALAFLKEHGLEEGGFLC
                     CIPRLRYTPYWVIPEKKAKPDPEKHARNEAMKEHDHAQLRQAIIEVVRQTGMKVLVCP
                     EDKTQMAVGKEMLYDPLPEDVKKRVAWRPGYWLTGEALSTYARSAGLFGNEMHSPIMC
                     IGAGIPAIVCRWAEQTSKGFMWRDIGLDEWLFDLDDEAQLPGIVPAVLTLAKDPVAAK
                     AKAAKARAYVAQRQARMMQAVGKG"
     gene            12603..13571
                     /gene="hemB"
                     /locus_tag="PLP58_04770"
     CDS             12603..13571
                     /gene="hemB"
                     /locus_tag="PLP58_04770"
                     /EC_number="4.2.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012420762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004655 - porphobilinogen synthase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porphobilinogen synthase"
                     /protein_id="HRJ07858.1"
                     /translation="MPIRPRRNRQSPAIRDLVRETVLRSADFIYPLFIQEGGANTPID
                     AMPGCQRWCLRDLVTEAGEAHALGIPAVVLFPRIPDELKTPDAAECHNDDGLVPRAIR
                     ALKAAHPALAVITDVALDPYNSDGHDGLVSPDGRILNDETLEVLCQQALCHARAGADI
                     VAPSDMMDGRVAALRSALDHEGHQAVSILSYAVKYASAYYGPFRGALDSAPKAGDKKT
                     YQMDPANAREALREAALDEAEGADMLMVKPAGPYLDILAALRATTTLPLAAYQVSGEY
                     LMIKAGAAAGWLDEEKVMLESLLGIRRAGADMILTYFAKKAAALLR"
     gene            complement(13588..15612)
                     /locus_tag="PLP58_04775"
     CDS             complement(13588..15612)
                     /locus_tag="PLP58_04775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009959251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HRJ07859.1"
                     /translation="MTRLSPFALLLALSSAVLADGPKDNLADNVRPIPPPGIEVPESD
                     AAALREGLARLRAAIDAAAKAQEKNPRLALLLPDIEIHHKAVDWALRYNEIHKPAELK
                     SAREILAEGMERAAHLKEGRAPWTAQKGLVVRGYRSRLDGSVQPYGMVVPESYSGAPA
                     RLDVWCHGRGETVSELVFMDQRRKQQGQVSPAGALVIHPYGRYCCANKFAGEADLFEA
                     LEHARQFYAIDEDRMVMRGFSMGGAAAWQFAVHYADHWCAANPGAGFSETPEFLNVFQ
                     SEDVSSIPWYQQTLWKWYNATDSAVNLFHCPTVAYSGEIDRQKQAADIMEKHLRQEGI
                     EVVHIIGPDTAHKIHPDSLIEIERRLAAIASLGRDRTPGEVRFTTWFLRYNQMHWVRV
                     DAMREHWQRARVHALLTGPAEVTVKTENVAALTLDMASGHCPISPMADPVIKVDGSTL
                     KAPRAKSDRSWLVHLRRDDAGKWALANTAHAPGKLVKKHGLSGPIDDAFMDSFLFVKP
                     TGASLHEQTGAWAGAEMDRAVFEWRRQFRGDARVRADKDLTEVDIAAHNLVLWGDPQS
                     NSVLARVIAKLPVGWTAEKLTANGKTHAAETHAPILVYPNPLNPEKYIVINSSFTYRE
                     YDYLNNARQVPKLPDWAVVDISQPHTSQAPGRIADAGFFDEAWQWRKETR"
     gene            complement(15826..18801)
                     /locus_tag="PLP58_04780"
     CDS             complement(15826..18801)
                     /locus_tag="PLP58_04780"
                     /inference="COORDINATES: protein motif:HMM:TIGR02604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HRJ07860.1"
                     /translation="MLRAILLSCLVLPALAEEFPAIYDTEKGAPMSAEEAAATMELPP
                     GFKCLVFAAEPDVRNPIGIAWDAKGRLWVAENYTYAENPMRWDTKLRDRVIILEDKDG
                     DGKHDGRKVFWEGGSYLTSVETGYGGAWVLHNGTLSFIADKNGDDVPDSEPEVLLDGF
                     NVKTIGHNIVNGLRWGPDGWLYGRHGITDTSAVGAPGTPLEKRTKINCGIWRYHPTRK
                     VFEVVCHGGTNSWGHDWNAEGELFWINTVIGHLWHGIPGAYYRRMFGTHLNPHVYEVI
                     EQTADHFHWDTGAESWSDLKKSGMSSKTDLLGGGHAHVGMLIYQGGLWPEKYHGAVLT
                     CNLHGNRINMDTLHREGCGYVGKHAPDFMKAKDKWFRGIDLLTGPDGNVFVADWSDSG
                     ECHDNDGVHRSSGRIYKIVYGNEKVIENSDFAAFDLPVAERAGVLVRMLQLGNNWWTR
                     MYFQKLAEDILASADSGEKMTAVEHAEKLVQSVKRAKSESERAKMVRMFAPGFDVAGL
                     PDWNVALKTFGNEYQKLAKDDPSGLVRLNVASSMQRLPLEARWPIATALAKHEEDAND
                     RQQPLMIWYGIEPAVAAEPMKGVELIREAKIPTVRRLVARRIAEMIEDKPETVDALVA
                     LMTERADVRGDMLAGMAAGLDGFSSAKKPAGWSDGVVEKWISEAPTPQNSNTPSLQGF
                     IRTLSALFGSGRAIEDLIVLVKDTDGDANARVNAFKGLTRATNPELLAVIRSLINDKV
                     LGTAARSALAAYDHPNIPKALLNPWPQRSEEQQAATVATLVSRASYAHALLDAVKAGG
                     VPKSAITPYQARAIRSLNEEALNAKLTEVWGELRDTPEAKKAEMAEWLAKLTPERLSQ
                     GDAIQGKAVFAAACAACHKLYGQGGQLGPDLTGSDRHKLTYLLENIIDPSAIVPADYR
                     MTVFQLKDGRTVTGVIPEQTERVLTIQTPAEKLTVEKSQVASQQQLPMSLMPEGLLTA
                     LGEEQAAHLFKYLMSSGPP"
     gene            18926..19786
                     /locus_tag="PLP58_04785"
     CDS             18926..19786
                     /locus_tag="PLP58_04785"
                     /inference="COORDINATES: protein motif:HMM:NF019474.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HRJ07861.1"
                     /translation="MKSLLFLSAVSLAAFSHAQEVKRDVPYVTGGHARQVLDIHAPAK
                     AEKLPVVFWIHGGGWQTGDKSSVQEKPRLFKERGCVFVSTNYRLLPEVAMADIIGDVA
                     AALGWVRANIARHGGDPEHIIVGGHSAGAQLAALVCTDDRHLKAHGVPFSALKGCIPV
                     DGDTYDVPAIIETAETRRRVHGQPQAQFGHREKFGHTAENHRDFSAVTHIAAGKSIPP
                     FLILHVSNHPDVSAQAFRLGAVLKAASVPVKVFGAKDTDHSKLNADLGLEGDAATGEV
                     LAFLDQVLGR"
     gene            complement(19796..20938)
                     /locus_tag="PLP58_04790"
     CDS             complement(19796..20938)
                     /locus_tag="PLP58_04790"
                     /inference="COORDINATES: protein motif:HMM:NF018038.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ThuA domain-containing protein"
                     /protein_id="HRJ07862.1"
                     /translation="MNLRHLLLAAAALTLAACSTPGAAKAKPGAAKAKPAAAEVKPAA
                     AKAKPAAAKVKPAAAEVKPAAAEVKPAAAEVKPGAGKHIVLLAGDEEYRSEEALPALG
                     RILQRQGFKCNVCFSAEEDGSINPDKGDSLTNPAALDSADAIIMLLRFRRWNEETLAR
                     FDAAVKRGIPVIALRTSTHAFAGIPAESAYAAWNWNKDGGWGKKVLGESWVSHWGRHK
                     HEATRGVVEAANAGHAILSGVKDVFGTTDVYEAAPPADATVLMRGQVLAGMTPDSPPL
                     ETLKATKDKVEQGINDPMMPIAWTRVVKNEAGGENKIFCTTMGAATDITNEGLRRMIV
                     NAVHWGLGTEAPAEMDLSLPEGYEPTTYGFKGYRKDLKVADHELGR"
     gene            21076..22533
                     /locus_tag="PLP58_04795"
     CDS             21076..22533
                     /locus_tag="PLP58_04795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009957936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ07863.1"
                     /translation="MRPLIFLLALAPGLLRAEILAGVAKVDITDRTVPVNDPCFAKAL
                     VLKSGETTAVLVTVDAVAIGGIGRIGNGFLANVRKELGGLADEVIINASHCHGIVRND
                     LQQLVVQAVKDAAKSLVPVKVSAGSGSESRISENRRMFLKDGTQTDMRRAYSMPRDED
                     VLSTGPIDPQIGLLRLDRADGTPLAIVYQFACHPIMNPPSKGNSADYPGFASKVIEEA
                     TGAMAFFVQGCGGDINPVHYKEVSRPADAEPLGTMLGASALAALRRIEPQADATLRVS
                     REVIALPRAADYEARITAIETEQKALVAALKPTNINFKTFLPLLLQHRLFPDMPSHYA
                     QGYLDNPEPLRQLDADNKALVEAYLGNLALMEKLTRLNANLALLKKHLADTKAANSPT
                     LDAEVCGLRVGAFKLVTFPGEVTVQVGLNIKKAAQDANAHVSGYTNGYIWYTATEQQR
                     RNTGYAQEDCDVMVAPEWQAIFEAKALQVLRELAC"
     gene            complement(22869..23879)
                     /gene="bioB"
                     /locus_tag="PLP58_04800"
     CDS             complement(22869..23879)
                     /gene="bioB"
                     /locus_tag="PLP58_04800"
                     /EC_number="2.8.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018970562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004076 - biotin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin synthase BioB"
                     /protein_id="HRJ07864.1"
                     /translation="MKYADLHRIYHQPFFDLIQQARAVHQEHWTGNEVQLCTLLSIKT
                     GGCSEDCGYCAQSARYSTGVQAEKLMEKDQVMERARAARASGSTRFCMGAAWKGVRAG
                     TRKFDQVLDIVREVSTLSMEVCVTLGHLGAEEAAQLKQAGVTAYNHNIDTSPEHYPNI
                     VSTHTFDDRLETIRHAQDAGMSVCCGGILGLGETIEDRLKMLEVLSNFNPHPESVPVN
                     ALMPMKGTPLGENVQVDSFSLIRMIAVTRIAIPRAKVRLSAGRTSLNREAQALAFFAG
                     ANSIFYGDKLLTAANPRANEDMALLKELGLAPQEPNPGMTAPAADEVRELAPACAIGC
                     GA"
     gene            complement(23892..24608)
                     /locus_tag="PLP58_04805"
     CDS             complement(23892..24608)
                     /locus_tag="PLP58_04805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009958839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ07865.1"
                     /translation="MNLPGALIFSALLLTSLPALAQNEYIICSGGPALRKWEDLRRAE
                     QQHDRWWGNFVRTARVRMQEIQRTQPQGTLVTWLVFRDGYVRRAAEDRDPLTSHVESV
                     RDTYKINLVWFRTGEEVINYINQGGGQIARNRHKISGFEFFGHSNKFCFLFDYSSDVY
                     AASAVWLHENDLRRLNRWAFARGAFCKSWGCHTGESMSKAWKRATGAPMIGAIGKTDY
                     SHMHQRNWQVALSPGSRWTQ"
     gene            24789..30626
                     /locus_tag="PLP58_04810"
     CDS             24789..30626
                     /locus_tag="PLP58_04810"
                     /inference="COORDINATES: protein motif:HMM:NF019323.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004866 - endopeptidase inhibitor activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-2-macroglobulin family protein"
                     /protein_id="HRJ07866.1"
                     /translation="MKTSFGLSLLAALSLAATCASGQSAKSPPAARPVLMPELGIQPA
                     VLSPDSTIEVVFSTPMVGKELVGTKAENSPLIVKPALAGGFQWVSSRSGHFVLEQAPR
                     FGTEYEFSVRDGLRDESGALVPAGVLGTASSAGFQVRDQSPRWFYDEMALRLPKVMLE
                     FNDEVSAAEAARLAAFVSGEPALRVAAKVRHAKGADFTRYNEPDPTWAEEIAGVKPVL
                     APEATRLSALVIEPAEPLPVADDWTLHLPETLPNASGHATLAGAQVIELGSVKPLMVV
                     EARGHTPFDSPYYVDADFNKMLLPEREEEWKAEEIQALAEKLAAFVHVTPEVAGLKAT
                     VFDSTLRLSGPFALNTPYEVTVAPGILAADGLTLEEASVNEVVFIPNPPHVAAPAFVR
                     AQLAKGGAEYGIVAANVSSVRVRARRLTGPELLQAVAKFRDYESFYHKDEEDEKRAAF
                     KPAPLDEYPGALVFDRSFQINKPLDQSEIIKLNWREVLGDNTAAPLFLECEGRAMEGL
                     GARGVLTQTLVQFTDIGLMQKSNGRETLVFATSLQTGQPMPGARLTLVDSEMRLMGYA
                     DTDASGLAVVKGENPAHILAEKAGDCAVIRCEDSHIGHVIPYDIPTAWENVWKPQIQT
                     FVFSDRPLYRPGDTMHIKALCRVRTADELALPAEGMKASVTLRDPRYRVVLQKEVVLS
                     AGGSWSDDIKLPPGPLGAYNLKINPVSEDDDSGSSGGHLYFRIEDYKPNTFEVALKKD
                     ELEIQPDRLRLPLTAKYFMGKALTRSKVEWTAYSLRQFSPPEPWSQFHFGDAPSWARY
                     GKDRDADGYYDDSDSDEESEWWVDGTAYLDDEGRAVLEMPVPPPDRAALPQQVRVTAE
                     VTDINQQTISASAEVEVPGADFLLGLKGPDFFGTAGAETQVEIVAVDSRGRPVAGGIR
                     AEVLVERQEYHTLKIATAGGGTTTKDQVILREELKQSVPLQAATPGSAPAAVIRFKPA
                     RGGAYFVTATTQDAQGRKVLSRMPLYVIGGGEFPWAMEDGARINLQPEKKTLKPGEEA
                     VIVVKTPIAGTALVSVERNKVHRHFVTQISPDQPVVRVPIQEEEAPNVFVSVLVIRGS
                     EASPKQHKMPEYKLGYCELLVDSKAKVLAVDIQPASEEVLPGGELPVSLTVSDAKGAP
                     VAGAEVALYASDEGVLSLVSHQTPDPSAYFHEQRPLAVDNHTSFDDLLDEDSSARSRG
                     NKGFLIGGGGEEGIADVLVRKNFVATPLWIATALTDAQGRVSTTVKAPDNLTRYRLMA
                     VVTHGADRFGHGEAAFKVNKPLMIEPVVPRFARLDDEVLLKAVVHNTTAHEGEVEVRL
                     ELDETADFIRDERLFIPVSLARKPAEGEKSWTQTVTLKAHETAAVAFPARFVKLGSAD
                     WKWTARTVKWSPGAPALSDGSVSTLEVRHPVPELKEVRYARVSAAGPPADLAAKINPA
                     LLEGEGAVRVALSTTRLFEVQDALDYLLSYPYGCAEQTTSATMPWLALGGYHTLFPAH
                     LSADKARAAIQNGVDRLLQMVTDRGGLAYWPGGSEPSLWASAYGGFVLLRARSAGALV
                     PEETVNDLVQYLVKSLRGLEKEADFYRVTDAALALYTLARAGHPQESYQNLLHARRDQ
                     LPETARLYTALAMCLCNSPAAQIKDMLAAPAVKKGAKPLPAWSYWAGSGVNPALRLIV
                     CTHLGLKKDAEDLALSILNSRNGRGEWGNTFTNAWTLHALTAYERSLKPSAEPLLARV
                     QWGAREAEINVPPHPGSAQVSLPLDARLTAAPMRLTLPEQRQGFTRVEARAFPPARDF
                     QGENKGYAITRSYAKLLDRGELGGADDLRVGDMVVVTLWIEIGQGAHRYLAIDDALPG
                     VLEAINPNFDTQNQRQGAQLPPGIQAWHCDHREIRADRALFFTNYAPRGGKFQLRYLA
                     RVIAEGDTTAPPARIEAMYEPHRYGLSPAQRLKTLPSSGGQVAGR"
     gene            30713..31840
                     /locus_tag="PLP58_04815"
     CDS             30713..31840
                     /locus_tag="PLP58_04815"
                     /inference="COORDINATES: protein motif:HMM:NF018613.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ07867.1"
                     /translation="MILVLTAGFGDGHNTAASSTAGAVRRLHPGEEVVTCDLVSEALP
                     AVAAVLKYLYQQAITHMPSAWRLVYRMLEKSDFDPDKQPWLLPLVRHLRDKIEALKPR
                     VIISTYPVYAALLQVLRHQGVSPPPVVTIITDSVSIHRVWLMQPSDLYCVADEESRSV
                     VIGMGVPADKIRVTGFPVSLQFIEPLPGEAEKRADNGRILYLPSTPARHVAATLRELR
                     PLLENGARLTLPAGKHKQRLYHVLRRFTDAHPGVQLDIIGWTTRIPELLRTHDVVICK
                     AGGAIMHEVLAARVPAVIDYVVPGQEEGNAEMLLSRRCGLRSHSPRETAKAVESILAE
                     DCRLGREMSANMAPISVPDAAMRTARAALEAATPGLARDAG"
     gene            31888..32469
                     /locus_tag="PLP58_04820"
     CDS             31888..32469
                     /locus_tag="PLP58_04820"
                     /inference="COORDINATES: protein motif:HMM:NF017496.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Uma2 family endonuclease"
                     /protein_id="HRJ07868.1"
                     /translation="MIDLLNQPGFQKRVIPLSVKAWHQMIAKGLAPERAELIRGVIVE
                     KMPKSILHIKLAGRLFILLLEALGGSFWVRKEDPLSLEDSEPEPDLSVIEGREEEFNS
                     HPSTAKLVVEVSVSTLAEDRALAPIYAEAGVEEYWIINGRERCLEVFRKPGTSGYEIQ
                     ETHSAGQTLLCAALPGVSVDVSALFQGLPEAEA"
     gene            complement(32476..33774)
                     /locus_tag="PLP58_04825"
     CDS             complement(32476..33774)
                     /locus_tag="PLP58_04825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009961823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1501 domain-containing protein"
                     /protein_id="HRJ07869.1"
                     /translation="MKATNELSRRTVMTRMAQSCLGVGLLPSLEHLFSSKALAAGEGA
                     ATAMQAPTARNVIFLYMTGGQSHLDTWDPKPGNTEVMGATKAIKTSADGVLIGEYLPR
                     TARLMHQACVINSMATNTAAHEQANYFMHTSYGLRSTIKHPGLGSWLSFYQGPGNPDL
                     PPYVFIGNDSRHPGAGYMARKHGPLMINNPETGLQNVRPQKGVTDDRFAMRRDMAQAL
                     DAEFQNRWDVPGVKAYADMYDDAVRMMKSEDLVAFDLTKEPADLRAKYGRETFGQGCL
                     LARRLVEHGVRFIEVSLGSWDTHTANFISTPRLCETLDTALSALVQDLDSRGLLQQTM
                     IVLASEFGRTPKINQNQGRDHYPIFSSVIFGGGIKGGIKYGATDKDGAQIAADKVGPP
                     DLNATIGYALGLPLCQVIYSPSKRPFTVADKGQPLTHLFA"
     gene            complement(33804..35969)
                     /locus_tag="PLP58_04830"
     CDS             complement(33804..35969)
                     /locus_tag="PLP58_04830"
                     /inference="COORDINATES: protein motif:HMM:NF019223.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1549 domain-containing protein"
                     /protein_id="HRJ07870.1"
                     /translation="MKALLTSAFAVLCLTTTPHLHAADTSAASKKIDELLAAGWRKQK
                     LQPNAPVGDEVFLRRAYLTVVGRIPTYGEAAAFLKSEAPNKRAQLVDALLESEGYTHH
                     FLNYWSDVLRAQSAGVAGSTTAQNYLNFLRDSLKTNKPYDQLARELVSSAGTCFDTGA
                     IGYYMRDRGMPLDNLSNTTRVFLGTRMECAQCHDHPFDKWTQKQFYEMAAFTHNMTSG
                     SYRSTTADETNKMIRADKTLDKETQDLMRQAITEVVRPLRDTQVVQNKAAIRLPHDYK
                     YSDAKPKDAVPAAVMFGKPVEIGKETDHSKAFAEWLTSPENPRFTNLIANRLWKKVFG
                     LALIEPLDDLMDSSTASNPELMKYLEQQMVALGYDMKAYLRMLLNTQTFARESIKEVG
                     MGEPCYFQGPVFRRMSAEQAWDSMIALISPVPDQSNWSARERERVESEKRHLLARMLD
                     KTEPALLYQAAKEVAGAMRVQNKEFDQLRKELDEARAKEDKAKISEIQRRLGQSQRIL
                     RETVSRCFHAAAEKSGNAEVKQMLAEMSGGGEMEMAMMNAMETPRLEVRERDLEGALK
                     ERLLKDAEMLGYKDEKSRKGYEGYLKTLHQTWVRAAELPSPAPRGHFLREFGQSDREV
                     IENASDEASVPQALLVMNGSIGSQITSGWSALMLNLRKAKTPDDKLDVLFLSLYSRLP
                     SDSERALIRQRMESYAGSKTVWEDLALAAVSTQRFIFIE"
     gene            36206..36772
                     /gene="efp"
                     /locus_tag="PLP58_04835"
     CDS             36206..36772
                     /gene="efp"
                     /locus_tag="PLP58_04835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009963873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HRJ07871.1"
                     /translation="MPSTPVINLRKGHAVRYNNEVCLVSDFELKTPPRMASYVQMSVR
                     TLSTGKVYNLRMTSNESLESVNLNREPHEFSYKDGDDYHFMHPETFEDVILMGDQVRD
                     AKNYLIEGQKYTVQFADDIVIGIELPPSVIMTVTEAPEGVKGDSANNVYKAAILETGL
                     QVQVPLFIGPGDKISIKTEDNSYLGRAN"
BASE COUNT         6248 a        11863 c        12216 g         6599 t
ORIGIN      
        1 cggcgcgccg gtcgccggcg ccggcggcgt gcctcatgcg cacgcggctg acggtgagca
       61 cggcgctctc cagccctgag agggtgaagg agacggccag gcagagcgcg aggagcagca
      121 gccagctcat gctccggcct cctccacgct ttgcagttcc gcgcgacgcg cctcgtccag
      181 gcgcagctcc acctcgatga tgcgggcgcg gacgacgcgc cggaccttga gggtgatgcc
      241 gtcgaggcgg gcgcgctcgc ccgccttcgg catgtggccg aggtggttga agacaaatcc
      301 gccgatggtg tccacaccct cgtcggggat ggcggcgcgc agctcgcggt tcacgtcgtc
      361 aatgcgggtg ccgccgtcca cgatgtagcg cccgccgccg aggtggcgga tctcgcttgc
      421 ctcgccgagc cagggcgctg cctcgtgcag cagccagtcg gcgatctcct cctgcccgac
      481 catgccttcg aggccgccgt attcgtcggc gatgagcacg ggcatggcgg tgcgcttcag
      541 gtgctggtcg agcgcgtcga gcacgggcag ggtctcgggg acaaaagacg ggctgaagag
      601 catgccgcgc cagtcggggc gtcccgcgag cttccaggcc tcggtgtcga gcacgccctc
      661 gaccatgtcg ggtgtctcgc cgtgaaagac gacgaagcgg ccggaggact gctcgagggc
      721 ggcggcgatt ttctcaggct tgtcgtccac ggccatgagg gtgaggtcca cgcgcggcac
      781 catgcagtcg cgcacggtga gggtttcgat gtcgagcgcc tcgcgcagca tttcggcctc
      841 gtcggcgccg agcaggccct gctcctggcg catttcgacc agggtctcga actcgttgcg
      901 ggtgatcttg gggttcaact tcacgcggct ggggatgacg cgcttgagca gcagatcggt
      961 gattttgtcc gcccaggcgg cgaaggggtc gagcacgggc tgcagcggca ccagcaggcg
     1021 caggccgccc tggagcacgc tggtgggcgc gcgggcggcg aggaatttcg gcagcacatc
     1081 gccgacgagc actgtggcgc agaagatgag cgcggtggcc gcccaggggt tccaccctgc
     1141 ggcgcgcaac ggaccggaga ccaggtgcag gccgagggcg gccagggcca ggttcaaggt
     1201 ggcggagagc agcagggcgc ggtgcaagtg ggcgaagggg ttcgcggcga tgctgcgcag
     1261 cttttgtgcc atgccggggc cggcggcctc caggcgcggc tcgatgtcgc gcgcgctgtg
     1321 aatggctgtc tccacggcgg aaatgacggc cagcagggcg agcagggaga gatacaggat
     1381 ggcggcggcg agcgtcactg cggccagtgt cagaaggatg cggcgggggt caagcgccaa
     1441 ggctgggacg gacgccgggc ggcactcacg cgccggcgcc tatggtgacg cgccttgcgg
     1501 ggacggggga ctcgatgatg ccttcagcgg tctcctgctt gagacggagc tggccgcagg
     1561 cggcggcgat gtcgtggcct ttttcacggc gcagggtggc tttgacgccg gccttcaaca
     1621 gcgcgccgag aaaggcgtcc tgacattcct cggaggggcg cgtccagggc agtccctcga
     1681 cggtgttgta ggggatgagg ttcaccttgg cctccagcgg gcgcgccagc cgcgccaggg
     1741 cgcgggcctg ctcaaggctg tcattcacat tctcaatgag gatgaactcg aaggttaggt
     1801 gctgtttgcg cttttcatgc cagacctcca gggcggcgaa gagctcgcca agaggatgct
     1861 tccggttcac cggcatgatg cggtcacgca cctcgtcggt ggcgccgtgc agggagatgg
     1921 ccaggcggat ttgcaggggg aagtccgcca gcctgcggat ctgcggggcc aggccgctgg
     1981 tgctcacggt catgtggcgc gcgccgatgc cgacgcccca ttcggcgttg aggatttcga
     2041 tggccttggt gacgtgcgag taattcgcca gcggctcgcc catgcccatg aacacgaggt
     2101 tgtccacccg ctcgccggtg tgctgctcga cctggacgat ctgctcgacg atctctgcgg
     2161 cggtgaggtt gcgcgtgaag ccggccaggc cgctggcgca gaatttgcag tcgtaggcgc
     2221 agcccacctg gctggagacg cagagggtgc ggcggtcgga cttgccgccg tagagcgcgg
     2281 ggttcgccgg aatgagcacc gtctccacat agcgtccgtc gctcaggcgc aggaggaatt
     2341 tccgcgtcgt gtcctttgat cccgtgaccc gggcgatgct catggtgcgg gtgacgaagc
     2401 gctccgcgag cgcggcgcgc agggccttcg gcaggctgga catgccctcg atgccggcgg
     2461 cgcgcttttg gaaggtccac tccagcacct gccgcgcgcg gaaggccggc tcgcccattt
     2521 cagtgaaagc ggcggccagc tccggcggag tgatgccgag cagggaggct ggaggctggg
     2581 ggtgggacac aaggcgtgtg ctaacgcctt cggcccggct tttcaagctc atggacccgg
     2641 cggacgcgcg ggagagggcg cgcacccggc tgttgagctt ccggcgcgca ggctgtaagg
     2701 ggtggcccgc cgccccgcag gatgaaagac acccttgaag acctcgaaca atacctcatt
     2761 gaggtgatca tccaccacaa gcgtggcatc cggccgacgc tgctgcgcgc ggctttcagg
     2821 ctgctttccg gcatttactc cggcgcggtg cggctgcggc ttttcctcta ccgtgagcgc
     2881 tacatccacg accaccatct gggcgtgccg gtgatcagca tcggcaacat caccgtcggc
     2941 ggcacgggga agacaccggt ggtggagctc ttcgccaagg cgctgcacga gcgcgggcgg
     3001 cgggtcgcca tcctgagccg gggctacaaa agcaaacgcc agcgcaaaat cccgctcggc
     3061 tggcgcatcg ccgcgaagct gggattcgcc cgcaaaccgc gcgagccgct gccgcgcgtc
     3121 gtctcggatg gcgaacgcgt tttgctcgac tcctatgtgg ccggtgacga gccctacatg
     3181 ctggcgcaga actgcaaagg cgtgcccgtg gtggtggacc gcaaccgcgt gaaggccggc
     3241 gcccacgcca tccgcaaatt cggcgcggat gtgctgctgc tcgacgacgg cctgcaatac
     3301 ctcaagctca agcaccggca cgacatcgtg ctggtggaca aaaccgcgcc cttcggcacc
     3361 ggccacatgc tgccgcgcgg caccctgcgc gagccgcccg cctccctgcg ccgcgccagc
     3421 tacatcttcc tcaccaagtc ggacggcgac agcggagagg tcattgagca aatccgcagg
     3481 cacaaccgcg tggcggaaat catcgagtgc cgccaccggc ccgtgcattt tgaggacatc
     3541 cacaccggcg agcgcctgcc gctggacgcc atgagccgca agtatgtcgg cgcgctctcc
     3601 ggcatcgccg tgccggagag ctttgaaaac gggctgcgca agctgggcgc gcgcgtgcag
     3661 tgggtgaagc gcttcaccga ccaccaccgc tttcaggaaa aggagatcac gcagttcatc
     3721 gaacgctgcg agaaggccgg cgcccatgcc attttgacca cggagaagga ctttgtgcgc
     3781 ttcccgaaac tgccgcccgg agacctgccc atctatttcc tgcgcgtgga gatcgagatc
     3841 acccgcggca gggacatttt cgacaagctc atccgcctca tcgccgagcc gcgccatgtg
     3901 actcaaggcc tggtgagcgc cgacctggtg gaggccacgt catgacggat gctgaaaacg
     3961 cccctgcggc gcacgcgctg gtgcgccgcg tcaccgtggg cggaggcggc gaggagcgcg
     4021 cggatgtcac cgcgcgcgag gagccgctgg agatccgcgt ggagggccgc agcgtggcgg
     4081 tggtgatgcg cacgccgggg catgacgagg agctggcggc cggctttctc gtcagcgagg
     4141 gcgtgataaa aacggcgcgg gacattctgg aaatctccca gtgcccaagc atgggcaaca
     4201 cgcatggaaa catcgtggac gtgcttctcg gcggcgcggt ggtgaactgg gagtcgctga
     4261 cacggcatgt tttcagcgcg tcgagctgcg gcctgtgcgg caagacaagc atcgagtccg
     4321 tgttccagag ctttcccgcc gtgacggcgg acgcgcgggt cgaggcgagt gtcatcgcct
     4381 ccctgccgga gaaactgcgc gccgcgcagg agaccttcgc cgccacgggc ggtcttcatg
     4441 ccagcggcat ttttaacctg gagggaaacc tgctcgtgct gcgcgaggat gtgggccgcc
     4501 acaacgcgct cgacaaggtg ctcggcttcg ccctgcaaag aaacctgctg ccgctggacc
     4561 ggcacatcct gctggtcagc gggcgcgtgt ccttcgagat catgcaaaag gcgctggctg
     4621 ccggcgtgcc cgtggtggcg gccatctccg cgccgagcag tttggcggtg gacttcgcgc
     4681 gcgagggcgg ccagactctc atcggctttc tgcgcggcgg cacgatgaat gtgtacaccc
     4741 atcccgcccg catcctgaat tcctgaaccc cctctccacc catcatgtcc agcaacgtca
     4801 aaatcgtcgg agccgaggct ccccccgcgc cccgcgccca gccggcaccc gcggccgcct
     4861 actcgaatgt cagccgcgtg tttgccacgc ccaatgaagt ggtgctcgac ttcgccatga
     4921 atctgaatgc cttcggcccg ctgatcgagg aggacgcgcg catcgtcagc cgcgtggcca
     4981 ccagctacga cggcgccaag cgcctctggg tccacctcac ccagacactc caggcctacg
     5041 agcagaagta tggcgtcatc gagctggact tgtccaaacg cgcgaagaca gccgacgcgg
     5101 gcgctgtgga gaaatgaatc tgccgaatga cttgattttc cctcctgttt tccgatgatc
     5161 gaaaattcgt cccatcgccc gacccgatga aattctgcat tttcggagac atccacgcca
     5221 atctggaggc gctgcaaagc gtgctctggg acgcgcatga gcagggctgc gccagccatg
     5281 tgtgcctggg cgacatcgtg ggctacgcgg cgaatccggc ggagtgcctg gagaccgtgc
     5341 gggagatgaa ctgccccacc gtgcgcggca atcatgacga gggcgcagcc agcaccagca
     5401 acctggagga gctgaacccc ctggcccagg ccgcgctgct atggacacgc gagcagctca
     5461 gcgaggagca gcgccagtgg ctgcgtgacc tgaagctcgt gcgccaggtg cgggatttca
     5521 ccatcgtcca tgccacgctc gactcccccg gaagctgggg ttatgtcacc aaccgcttcg
     5581 acgcgatggc cagcttcagc taccagttca cccaggtctg tttctacggc cacacccatg
     5641 tgccgcgcat ctttgaaaag gacgactccg tgcgcgcagc ccgcggcaac gaggtgacgc
     5701 tgcagcgcgg cgtgaaatac ttcgtcaatg tcggcagcgt cggccagccg cgcgacggcg
     5761 actggcgcgc ctcgtatgcc atctatgacg cgcaggcgca gaccatcgcc atccgccgcg
     5821 tcgagtatga cattcagacc gcgcaggaca aaatccgcgc cgccggcctg ccgagcctgc
     5881 tggcggagcg gctggccctg ggcaagtgat gaacacgcgg aaactgccgg acctggcgga
     5941 cttccgctcc ctgctggtgg tcaaacccag ctcgctcggc gacatcgtcc acaccctgcc
     6001 agccgtgcat gccatcaaaa acgcgcaccc gcacctgcgt gtgcgctggc tggcaaaccc
     6061 ggagtgggcg ccactcatcg aaggctcctc cgtggtggat gaggtggtgc cttttccgcg
     6121 caaaagcttc cgggggctgc tcggcggcat gcgttttctg aactggatct ccgcgcagtg
     6181 gaggcgccgc ccgcatccgg agccggagat cgtgctcgat ttccagggcc tgctgcgcag
     6241 cgggctggtc gcgcgcttga gcggttcgcg gcccgtcgtg ggactgtccg actcgcgcga
     6301 aggcgcgcgc ttttttcatg accatgtcat tgccgtcaat gccggggccc atgccctgga
     6361 gcgctatctg gaagtcccga gcgctttcgg catcccgttg gaaaagccgc gcacgccgct
     6421 gccggaggga accatgcccg ccggctggcc ggagcgcgag gatgcggtcg tggtgcatcc
     6481 ctggtcacgc ggcaaaggca agtctttgga acccgccgtg cttgaggcgc tctgcgccgc
     6541 gctggcaccg agcccggtcg tgctcgtcgg tgtgaggaat ggggtgtccg tgccgcgggg
     6601 cgagcacatc accgacctga gcgcgcgcac caccctgccg gagctggtct ggctgctgcg
     6661 ccgcgcgcgg ggcgtggtca gcgtggacag cggcccgatg cacatcgcag ccgctgtcaa
     6721 cgaccgcaca ctcggcatcc acacctggag tgatccgcgc caggtcggcc cgcacaaccc
     6781 gcgcgcctgg gtctggaagg acggacgcat cgcgcaccgc tgtgactttg ccagtgctga
     6841 atgcgccagc actagatcgc cgaacacgga ggatgctcgc gcgctggcgg agtttgtgaa
     6901 aaagctggcc tcatgaatcg cgggattgtt gctgtttccc gttttatttc gtggttccaa
     6961 ttctgaaaga ccccctacaa tgccgccatg aagcctgtct ttgaaaaagt gccgcgtcgt
     7021 gactgggagt cgtttcactg cgaggtgatc gaaggccccg actacggcac gcgctggcat
     7081 ttccatccgg agtaccagct caccctcgcc ctgcgcagca gcggacaccg ggttgtcgga
     7141 gacaatatag ccccgctcac cgacggcgac atggtgctgc taggctcgaa cctgccccac
     7201 atctggcatc aggacgcggg aaagggcggc gtggaggccg tcgtcatccg cttcgaggag
     7261 aacttcctcg gtcgcgattt catgaacatg ccggaggcgg agggcgtgaa gcgcctgctg
     7321 cgccgcgccg cgcgcgggct tgaggtgcgc ggacagacaa ggaaggaagt ggcgcagcgg
     7381 ctgcgccatc tggcggaggc cggcggcctg acgcgcgtgg tggacctgct ggccatcctc
     7441 gacatcctgg cctgtgccag ggacctccgc ccgctggcca gcgcaggcta cgaacccacc
     7501 ctgcacgcca ccgaccaggg ccgcatggag cgcgtgtgcg acttcattca ccagcatctc
     7561 accgaggaca ttgaccgcgg ccagctcgcc agactcgcgc atctgagcga gggcgccttc
     7621 agccgcttct tccgctcccg cacgggcaaa accgtgcccg agtacatcaa tgaggtgcgc
     7681 attggccgcg cctgccggct catcgccgag gaccagcaca acatcaccga catcgccctc
     7741 gattgcggtt accggaacct cgccaacttc aaccgccgct tccgcgaggt cgtcggacgc
     7801 actccgcgtg actatcgcgc ccagatgcgg acctttggcg gcgggcaggc gtgagttttt
     7861 tttcatgcgc ggccttcccg gctctccaca ttccgcgcat gatccggacc ggcatcggct
     7921 acgacgtgca ttgttttgaa actggccgcc ccctagtttt aggcggcgtg gtgatccctc
     7981 acagcatggg cttgaagggg cactcggatg cggacgtgct ttgccatgcc atcgcggatg
     8041 ccgtgctcgg cgccatggga ctgccggaca taggttttta tttccccccc tcggacatga
     8101 gcatcgaggg catctccagc ctgcgcattc tggaaaagtg cgcggcgctg gccaaagagc
     8161 acggctttgc catccacaat gtggacagca cgctcgttgc cgaggcgccg aaggtgatga
     8221 aacacgcgcc gtccatgaag gagaacatcg ccgccgcgct gggcatcacg ccaggccagg
     8281 tgggcgtgaa ggccacgacg aacgagcgca tgggcttcgt gggccgggag gagggcatcg
     8341 ccgcgctggc ggtggcctgt gtcgaggggt gagcggcgcg ctcagtgtga acgcgcgatg
     8401 acgtccttga gcacggcggc gagtctgagc ccggcggtca ccatctgctg ccgcgagaca
     8461 aagcgcgcct cgcgaacata ggcgtcgcgg tcgagcgtgg tcatcgagac catcatgacg
     8521 ttgccgccgc ggctgctgcg cgtgtttggc tgcgcgctgg ttgaggcaag acggcgcagc
     8581 agcgcggggg aatacacatg gctttccgcc agggcatggc cttccttcaa ccattccagc
     8641 gggtccagct cgcgcgcggc ctcggcgccc ttcgaccaga gtgcggcatc cgccttgagc
     8701 ggcgcaagac gggcgctgac atcggcgagg ccgttttttt catcgttcca gaggctgtcc
     8761 caaaaagcgt gcagcacatc gctggtgagc acgggctgcg cggacttcaa accgaggatc
     8821 atgatgcggt tggcgccctc gcagccgtcg ggcagttttt cagggatgaa aatctgcgcg
     8881 cagtggcagg gctggtgcgt gtctccgagg cagtgaaaga gccagcaaag gttcacaccc
     8941 tgcccgccgt gatcgcgtgc gggatcggcc agctcatgga gcaagcgccg gagtgtctgg
     9001 atggagttga agccctcctc cggctcggtc atgccgggct gccagtccat gggcggcggg
     9061 gcggctttgg ccgcaagcgc cgcgcgtgcc gcattgtccg ggaagacggc caggtcggta
     9121 tagtgccagg tgctgtggtg gtgcgcggcg ttgatctcct tggcgttcgg gtagccgccg
     9181 tcacggcgaa tgaggtcgga ccagatcgcg gcctgggcga agaaccagcg cgcgcgggtg
     9241 tccgcgtcgg gcgcggcgga ggcaaactcc tcgcgcaacg gcagctcgaa gagctcctcg
     9301 cgcgtcggat gcgcctccag cctcgtcatc acccaggcgc gctcgctctc gctgagatgg
     9361 tcgtaggcga tgagggcgat ggctttatgc ccgccatccc accatgcccg cgcgggctga
     9421 accaccatga aaacaccgat caagaaactg ggaaaacgca tggcccaggg tgaagctgga
     9481 aacacacccg cgcaactcgt ctggcggcat tgccacatcc cgcgttgacg ccgcccccgc
     9541 gcgcggctga atgccccgca tgtccgacac cgaccccaag cgcacccggc tgacgatgcc
     9601 cgaatacgcc atggccctgg cccacgtggc cagcctgcgc tccgaggacc cgtatcgaaa
     9661 agtcggcgcc gtggccattg acttcgacaa ccgcgtcatc ggcaccgcct acaacggcct
     9721 ggcccctggc tacaatgcca gccccgaatt ctggcacgac cgcgacgacc ggcgcaaata
     9781 catgctccac gccgaggtga acctgtgcag cctcttcacc cgcgggaacg tgaagcttgt
     9841 ggcatgcacc accaagccct gcaccagttg catgcaaatg ctctgcgcct acggcgtgcg
     9901 ggaggtctat taccgcgacg actaccccga gtctgaggcc gacgccatcg cgcagcgata
     9961 cagcatccag ctcgtccgcc tcacagacta cccgcgcatc atcgccggct tcgaggccgg
    10021 gtgagcacgg cgcggaccag gagtcacgct cgttcaggat tgcggcggtt ttggcagtgc
    10081 tttttcacgg caccatttcg agaagtctcc ccgcagggct tgctgcagtt ccgagggcga
    10141 acaatcaaac aaccggcatg cctcgtctct caggatcaga tgagccttgc gcttgtcgtc
    10201 cgctccctcg cctgtggccc acagggcgtc gtaccaggcc gcgtggagct cttcaatgag
    10261 cttgtgcctc gccattcaat tcaagcagct tttgttgaat ggaccgaatc ctgtccggcg
    10321 agctttctcc atgccactcg tcccagttgg agcgcaaggt cgcgagcgcg gcctcggcat
    10381 tttccccgga aaaacccagt cgcgacaatt cctcccgatc ccccgactcg gcaatatgaa
    10441 gcagcaaaaa ctcgccaatg ctgatcaacc ctgtgagaca ggcgcgatgg aatttgaaat
    10501 ccaccgctcc cggttgtttt tgacgcatca gaatgaactc agcctgcctg aaaaaacgga
    10561 cagcgatgcg ccattgagcc catagtgcca cgaggtgctc atgcctctcc tgctcctgga
    10621 catccgcgat cagacggcgc acccagtccc attccgaagc ggacactccc tcaagtctca
    10681 tggtcatttg ctcttccata tcatgactgg ttttacggcg gaatgatgat gttttgatgt
    10741 catcaagtga catctatgca gcttgccatg cctttggcaa gttccggggc cattttcaac
    10801 tcctcaaaga ccacatcgaa gcctttggct agcgcgagca cggcgggcac gctggccgca
    10861 tgaatccagc caccccccat tttccccagg cggcggattt cccggtcgag ggccgggcca
    10921 tcaatgagac gcgccagttc ggagcggctg agcatgagcg gcgcggactt gggagttcat
    10981 ctgcacggac atggcagcct gggaaccggt gtcctgaagc tggcggcgca cttgttgata
    11041 ggcttcctca tagctcggct tccgtcccgt tttcggcggg aggcgaagcc gggctcccgg
    11101 cggggggagc tggatgcttt ggagatcaag agccatgcgc ggcaagacta cgaccccgcc
    11161 caggcgctgg caagggcggg attcctcacc ccttccccac ggcctgcatc atgcgcgcct
    11221 ggcgctgggc gacgtaggcg cgggctttgg cggctttggc cttggcggcg acggggtctt
    11281 tggcgagagt tagcacggcg ggcacaatgc cggggagctg cgcctcgtca tcaaggtcga
    11341 agagccattc gtccaggccg atgtcacgcc acatgaagcc tttgctggtc tgctcggccc
    11401 agcggcagac gatggcgggg atgcccgcgc cgatgcacat gatggggctg tgcatctcgt
    11461 tgccgaagag gccggcgctg cgcgcgtagg tgctgagcgc ctcgccggtg agccagtagc
    11521 ccggacgcca tgccacgcgc tttttcacgt cctcgggcag cgggtcatag agcatctcct
    11581 tgcccacggc catctgcgtt ttgtcctccg ggcagacgag cactttcatg ccggtctggc
    11641 gcacgacttc gatgatggcc tggcggagct gggcgtggtc gtgctctttc atggcctcgt
    11701 tgcgcgcgtg cttctcgggg tcgggcttgg ccttcttttc cgggatgacc cagtagggcg
    11761 tgtagcgcag gcgcgggatg cagcagagga acccgccctc ctccagcccg tgctctttga
    11821 gaaaggccag cgccttttcc tcctcccgca gatccgtggc aaaggcgccg tccgggccga
    11881 actccatgac cgggcaggtg cagccgaggc ttttggccag cgccagggag accgagtcgc
    11941 ggaagaagac aaagcgcgcg ccattgaaga cgcggagcgt ggcctgcacg gcctcgtccg
    12001 ccgcgtcccg cgtggaggtg gagccgcgcg tggagaaggt gattccgccg atgccgtagg
    12061 gcttgcccgt ggcctcgctc cagcggatga catcccgctg cgccacgagg gaggggccgg
    12121 agccgtgcag gaggaaatca cactcctcga aggcggtctg gatctccgcc tgcgacttga
    12181 ggatcttcag cttcgggaag cgcgccatga gcagctcgtc cacgccattg tccaccttgc
    12241 tcggccacag gcgcacctcc accccgggca ggtgcttctc caaaagcgcg agcatccccg
    12301 gcgtgtgggc gatgtcgcca atgttcaccg tctgccacga tgaccgcagc acgatgcgcg
    12361 ccgcgcgccc ctcccgcgcg gcgatggggc tgaggactgc ggcgagggcg gaggtgagaa
    12421 aggcgcgtct ttgcataaca ccgaagcctt ttcacaaagc cgcgcctccc gcaagcctgg
    12481 ggaaaaaacg aagctcgaat atcgaaattc gaaaactggg cttcgagctt cgaatttcga
    12541 atttcctcat tccactctcc ctttgctctc ccgcacgccc ctgccatcat gacgccatgc
    12601 aaatgcccat ccgcccccgc cgcaaccgcc agtcccccgc catccgcgac ttggtgcgcg
    12661 agacggtgct gcgctcggcg gacttcatct acccgctttt catccaggaa ggaggggcaa
    12721 acacgcccat tgacgccatg cccggctgcc agcgctggtg cctgcgggac ctcgtcaccg
    12781 aggcgggaga ggcgcacgcg ctcggcatcc ccgccgtcgt gcttttcccc cgcatccccg
    12841 acgagctgaa gacacccgac gccgccgagt gccacaacga cgacggcctc gtgccccgcg
    12901 ccatccgcgc cctaaaagcc gcgcatcccg cgctggccgt catcaccgac gtggcgctcg
    12961 acccctacaa cagcgacggc cacgacggcc tcgtctcccc ggacggccgc atcctcaatg
    13021 acgagaccct cgaagtcctc tgccagcagg ccctctgcca cgcccgcgcc ggggccgaca
    13081 tcgtggcgcc gagcgacatg atggacggcc gcgtggccgc cctgcgctcc gccctcgacc
    13141 acgaagggca ccaggccgtc tccatcctca gctacgccgt caaatacgcc agcgcctact
    13201 acggcccctt ccgcggcgcg ctcgactccg cgcccaaagc cggcgacaaa aaaacctacc
    13261 agatggaccc cgccaacgcc cgcgaggccc tgcgcgaggc cgcgctcgac gaggccgagg
    13321 gcgccgacat gctcatggtc aagcccgccg gcccctacct cgacatcctc gccgccctgc
    13381 gcgccaccac caccctgccc ctcgccgcct accaggtcag cggcgagtat ctcatgatca
    13441 aggccggcgc cgccgccggc tggctcgacg aggaaaaagt catgctcgag tccctcctcg
    13501 gcatccgccg cgccggcgcg gacatgatcc tcacctactt cgccaaaaaa gccgccgcac
    13561 tgctgcgatg acggcagact tcatccctca ccgggtttcc ttccgccatt gccaggcctc
    13621 gtcaaagaaa ccggcgtcgg cgatgcggcc tggagcctgg ctggtgtggg gctgggagat
    13681 gtccaccaca gcccagtcgg gcagcttggg cacctggcgg gcgttgttca ggtaatcgta
    13741 ctcgcggtaa gtgaagctgc tgttgatgac gatgtacttc tccgggttca gcgggtttgg
    13801 atacacaaga atgggggcat gcgtctcagc ggcatgggtc ttgccattgg cggtgagttt
    13861 ttccgccgtc cacccgaccg gcagtttggc aatcactcgg gcgagcacac tgttgctctg
    13921 aggatcgccc cagagaacaa ggttgtgcgc ggcgatgtcg acttcggtga ggtctttgtc
    13981 ggccctgaca cgggcgtcac cgcgaaactg gcgtcgccac tcgaagacgg cacgatccat
    14041 ttcagccccg gcccaggcgc cggtctgctc gtgcaaagac gcgccggtgg gcttcacaaa
    14101 gaggaagctg tccatgaagg catcgtctat cgggccggag aggccgtgtt ttttcacgag
    14161 cttgccgggc gcgtgcgcgg tgttcgccag cgcccacttg cccgcatcgt cacgacgcag
    14221 atggacgagc caggagcggt cggacttggc gcgcggagcc tttaacgtcg agccgtccac
    14281 tttgatcaca ggatcagcca tgggactgat ggggcagtgg ccggaagcca tgtcgagagt
    14341 gagtgcggcg acgttttcag tcttcacggt aacttcagcc gggccggtga gcagggcgtg
    14401 gactcgggcg cgctgccaat gctccctcat cgcgtccacg cgcacccagt gcatctggtt
    14461 gtaacgcagg aaccaggtgg tgaagcgcac ctcgcccggg gtgcggtcgc gtccgagaga
    14521 ggcgatggcg gcaagtcggc gctcgatctc gatgagagag tctgggtgga ttttgtgcgc
    14581 ggtgtccggg ccgatgatgt ggaccacctc gatgccctcc tggcgcaggt gtttctccat
    14641 gatgtcggca gcctgtttct ggcggtcaat ctctccgctg taggccacgg tgggacagtg
    14701 gaaaaggttc accgcgctgt cggtggcgtt gtaccatttc cacagcgtct gctgatacca
    14761 cgggatgctg ctcacgtcct cggactggaa gacattcaaa aactccggcg tctctgaaaa
    14821 gcccgcgccg gggttggcgg cgcaccagtg gtcggcgtaa tgcacggcga actgccacgc
    14881 ggccgcgccg cccatgctga agccgcgcat caccatgcgg tcctcgtcaa tcgcgtagaa
    14941 ctggcgcgcg tgctccagcg cctcgaagag gtccgcctcg ccagcgaatt tgttggcgca
    15001 gcaatagcgg ccatacggat gaatcacgag cgcgccggcg ggcgagacct ggccttgctg
    15061 cttccggcgc tggtccatga agacaagctc gctgacagtc tcgccgcgac cgtggcacca
    15121 gacatcgagc cgcgcgggcg cgccgctgta actttccgga acgaccatgc cgtagggctg
    15181 cacggagccg tcaagacggg agcggtagcc gcgcaccacg aggcctttct gcgcggtcca
    15241 tggcgcgcgg ccttccttga gatgagccgc gcgctccatg ccctcggcca gaatttcgcg
    15301 ggcggacttc agctcggcgg gcttgtggat ttcgttgtat cgcagcgccc agtccaccgc
    15361 cttgtgatgg atctcaatgt cgggcagcag cagcgccagg cgcggatttt tctcctgtgc
    15421 tttggcggcg gcgtcaatgg cggcgcgcaa tctggcaagg ccctccctca gcgcggcggc
    15481 atccgactcc ggcacctcga tcccgggcgg aggaatgggg cgtacattgt cggcgaggtt
    15541 gtccttcggt ccgtcagcaa gaacggcgga ggaaagggcg agcaacaggg cgaatgggct
    15601 gagtcgtgtc atgggaggga cctatcaacg cacggtccgc ccggctttgt ccacctgaca
    15661 ttgaagcggc gcttacagat tcacggaaag ctccagctcg tgtcatcaag tggtctcacg
    15721 gctgtgttgg agtgaagcct ttgggcggca cgggactgga ctccgctgcg ggaagtctgg
    15781 ggacaagcta aagcttgaac tccaacatga cgctgcgcgc ctggatcagg gcggcccgct
    15841 gctcatcagg tatttgaaaa gatgcgccgc ctgctcttct cccagcgcgg tgagaagccc
    15901 ctccggcatg agggacatcg gcagttgctg ctggctggcg acctggcttt tctcgacggt
    15961 gagcttttcc gccggagtct gaatggtgag cacgcgctcc gtctgctccg gtatgacgcc
    16021 ggtgacggtg cggccgtcct tgagctggaa gacggtcatg cggtagtcgg cgggcacgat
    16081 ggcgctggga tctatgatgt tttccagcag ataggtgagc ttgtggcggt cgctcccggt
    16141 gaggtcgggg ccgagctggc cgccttgtcc gtaaagcttg tggcaggcgg cacaggcggc
    16201 ggcaaagacg gccttgcctt ggatggcatc tccttgggac aggcgttccg gagtcagttt
    16261 cgcgagccac tccgccatct cggccttctt ggcctcgggc gtgtcgcgca gctcgcccca
    16321 gacttcggtg agcttggcgt tgagggcctc ctcattgagg ctgcggatgg cgcgcgcctg
    16381 gtagggggtg atggcgctct tgggcacgcc gcccgccttc accgcgtcga gcaaagcgtg
    16441 cgcgtagctg gcacgggaga ccagcgtggc cacggtggcg gcctgctgct cctcgctgcg
    16501 ctgcggccag ggattgagca gggctttggg gatattggga tggtcatagg cggccagcgc
    16561 gctgcgtgcc gccgtgccga gcactttgtc gttgatgagc gagcggatga ccgcgagcaa
    16621 ctccgggttc gtcgcacgcg tgaggccctt gaaggcgttc acgcgcgcat tcgcatcgcc
    16681 gtcggtgtct ttgacgagga cgatgaggtc ttcgatggcc cggccgctgc cgaagagggc
    16741 ggagagggtg cggatgaatc cttggagtga tggagtattg gagttttgag gtgttggggc
    16801 ttcagaaatc catttctcca ccactccatc actccatccg gctggcttct tcgcgctgct
    16861 aaagccatcc aggcctgcgg ccatgccggc gagcatatcg ccgcgcacgt cggcgcgttc
    16921 ggtcatgagc gccacgagcg catcgacggt ctcgggcttg tcctcgatca tctcggcgat
    16981 gcggcgggcg acgaggcggc gcacggtggg gatttttgcc tcgcggatga gctccacgcc
    17041 cttcatcggc tccgccgcca ccgccggctc gatgccatac cagatcatga gcggctgctg
    17101 gcggtcgttc gcgtcttctt cgtgcttcgc gagcgcggtg gcgatgggcc agcgggcttc
    17161 gaggggtaga cgctgcattg atgaggccac gtttaaccga accaaaccag aagggtcgtc
    17221 cttcgccagt ttctggtatt cgttcccaaa cgttttcaga gctacgttcc aatcaggaag
    17281 ccctgccaca tcaaatccag gcgcaaacat tcgcaccatc tttgctcgct cactttcgga
    17341 ctttgcgcgt tttacgcttt ggaccagctt ttcagcatgc tcgacagctg tcatcttttc
    17401 gcccgaatca gcgctcgcta gaatgtcctc ggcgagcttt tgaaagtaca ttctggtcca
    17461 ccagttgttt ccaagctgta acatcctgac taagacgccc gcgcgttcag ctactggaag
    17521 gtcaaaggct gcgaagtctg aattctcaat cactttttcg tttccataca cgatcttgta
    17581 aatccgcccg ctgctgcggt ggacgccgtc gttgtcgtgg cattctccgc tgtcgctcca
    17641 gtcggcgacg aagacgttgc cgtcggggcc ggtgaggagg tcgatgccgc ggaaccattt
    17701 gtctttggcc ttcatgaagt caggcgcgtg cttgccgacg tagccgcagc cttcgcggtg
    17761 gagcgtgtcc atgttgatgc ggttgccgtg caggttgcag gtgaggacgg caccgtggta
    17821 tttctccggc cagaggccgc cctgatagat gagcatgccg acgtgggcgt gaccgccgcc
    17881 gagcaggtcg gtcttgctgc tcatgccgga ttttttgaga tcgctccagc tttccgcgcc
    17941 ggtgtcccag tgaaagtggt cggcggtctg ctcgatgacc tcatagacgt gcggattgag
    18001 gtgcgtgccg aacatgcggc ggtagtaggc tccgggaatg ccgtgccaga ggtggccgat
    18061 gacggtgttg atccagaaga gctcgccctc ggcattccaa tcatggcccc aggagttcgt
    18121 gccgccgtgg cagacgactt caaagacttt tcgcgtggga tggtagcgcc agatgccgca
    18181 gttgatcttg gtgcgctttt ccagcggcgt gccgggtgcg ccgacggcgg aggtgtcggt
    18241 gatgccgtgg cggccgtaaa gccagccatc ggggccccag cgcaggccgt tgacgatgtt
    18301 gtggccgatg gtcttcacgt tgaagccgtc cagcagcacc tccggctcgc tatcgggcac
    18361 gtcgtcgccg tttttatccg cgatgaagct cagcgtgcca ttgtgcagca cccacgcgcc
    18421 gccgtagccg gtctcgacgc tcgtaagata gctgccgcct tcccaaaaga ccttccgccc
    18481 gtcgtgtttg ccatcgccgt ccttgtcttc gagaatgatg acgcggtcgc gcagcttcgt
    18541 gtcccagcgc atggggttct cggcgtaggt gtagttttcc gccacccaca gccgcccctt
    18601 cgcatcccac gcgatgccga tgggattgcg cacatccggc tccgcggcaa agacgaggca
    18661 cttgaagccc ggcggcagtt ccatcgtggc cgcggcctcc tcggcggaca tgggcgcgcc
    18721 tttttcggtg tcgtagatgg ccgggaattc ctcggcgaga gccgggagga caagacagga
    18781 gagcaggatg gcgcggagca tgggatgaaa aggcgggcga gtagaacgcg gcgttcggaa
    18841 aaaatttgcc gccggtcggg cgggtcccgc aagaagaaaa gacgctggca agcgcgccgc
    18901 ctcgaagcgc gtatgcgtgc ctgccatgaa gtccctgctt tttctctccg ccgtcagtct
    18961 ggccgccttc tcacacgccc aggaggtaaa gcgggacgtg ccttatgtga ctggcggcca
    19021 cgcccgccag gtgctcgaca tccacgcgcc ggcaaaggcg gaaaagctgc ccgtcgtctt
    19081 ctggattcac ggcggcggct ggcagacggg ggacaaatcc agcgtgcagg aaaagccgcg
    19141 cctgttcaag gagcgcggct gcgtctttgt ctccaccaac taccgcctgc tgcccgaggt
    19201 ggcgatggcg gacatcatcg gcgacgtggc agcggcgctg ggctgggtgc gcgccaacat
    19261 cgcccggcac ggcggcgacc cggagcacat catcgtcggc gggcactccg ccggcgcgca
    19321 gctcgcggcg ctggtttgca cggacgaccg ccacttgaag gcgcatggcg tgcccttctc
    19381 agcgttgaag ggctgcattc ccgtggacgg ggacacctat gatgtgcccg ccatcatcga
    19441 aacggcggag acgcgccgcc gcgttcacgg ccagcctcag gcccagttcg gacaccggga
    19501 aaaattcggc cacacggcgg aaaaccaccg cgacttctca gctgtcaccc acatcgcggc
    19561 gggcaaaagc atcccgccct ttctgatcct gcatgtctcg aatcatcccg atgtcagcgc
    19621 gcaggccttc cgccttggcg ccgtgctcaa ggcggcttcc gtgccggtga aagtcttcgg
    19681 cgcgaaggac acggaccaca gcaagctcaa cgccgacctt ggcctcgaag gcgacgcggc
    19741 caccggggaa gtgctcgcct tcctggatca agtcctcggg cgctgagccg cgcgctcacc
    19801 ggcccagctc gtggtcggcc accttgaggt ccttgcggta gcctttgaaa ccgtaggtcg
    19861 tgggctcgta gccctcgggc agggacaggt ccatctccgc gggcgcctcc gtgcccaggc
    19921 cccaatgcac cgcgttcaca atcatgcggc gcagcccctc gttggtgatg tccgtggccg
    19981 cgcccatcgt ggtgcagaag attttatttt cgccgccggc ctcgtttttc accacgcgcg
    20041 tccaggcgat tggcatcatg gggtcgttga tgccctgctc caccttgtcc ttggtggcct
    20101 tcagcgtctc cagcggcggg gagtccggcg tcatgccggc gagcacctgt ccgcgcatga
    20161 gcacggtggc gtcggcgggc ggcgcggcct catacacatc cgtggtgccg aagacatcct
    20221 tgactccgct gaggatggcg tgccccgcgt tcgcggcctc gacgactccg cgcgtggcct
    20281 cgtgcttgtg ccggccccag tggctgaccc aggactcgcc cagcaccttc tttccccagc
    20341 cgccgtcctt gttccagttc cacgcggcgt aggcgctctc cgccgggatg ccggcaaagg
    20401 cgtgcgtgct ggtgcgcagg gcgatcacgg ggatgccccg cttcacagcc gcgtcgaacc
    20461 gcgccaaagt ctcctcattc cagcggcgga agcgcagcag catgatgatg gcgtccgcgc
    20521 tgtccagcgc ggcggggttg gtgagactgt cgcccttgtc ggggttgatg ctgccgtcct
    20581 cctcggcgga aaagcacacg ttgcacttga agccctgccg ctgcaggatg cgccccagcg
    20641 cgggcagcgc ctcctcgctg cggtactcct cgtcacccgc cagcagcacg atgtgtttgc
    20701 ccgcgcccgg cttgacctca gcagcggcgg gcttgacctc agcggcggcg ggcttgacct
    20761 cagcggcggc gggcttgacc ttagcggcgg cgggcttggc cttagcggcg gcgggcttga
    20821 cctcagcggc ggcgggcttg gccttagcgg cgccgggctt ggccttagcg gcgccgggcg
    20881 tggagcaggc ggcaagggtc aatgcggcgg cggcgaggag gagatggcgg aggttcatgg
    20941 ctgggcgggg tggatgaagg cggcggagaa aacaggaatc aagggcgcat atttcaaacc
    21001 gttctccgac gcgcgagcgg agagaacctt catgcgcggc acgaaagcgg cgcgcgcgga
    21061 gcgtttgttg ccttcatgcg cccgctcata ttcctcctcg ccctcgcccc cggcctgctc
    21121 cgcgccgaaa tcctcgccgg cgtcgcgaag gtggacatca cggaccgcac cgtgccggtg
    21181 aatgatccgt gttttgccaa ggcgctcgtc ttgaagagcg gcgagacgac cgcggtgctc
    21241 gtgacggtcg atgcggtggc gattggtggc atcgggcgca tcggaaacgg cttcctggcc
    21301 aatgtgcgca aagaactcgg cggcctcgcg gacgaggtca tcatcaatgc cagccattgt
    21361 catggcatcg tgcgcaatga tttgcagcag ctcgtcgtgc aggccgtcaa agacgccgcg
    21421 aagagtctcg tgcccgtcaa agtcagcgca ggcagcggca gcgaaagccg catcagcgaa
    21481 aatcgccgca tgttcctcaa agacggcacg cagacggaca tgcgccgcgc ctactcgatg
    21541 ccacgtgatg aagacgtgtt gagcaccggg ccgattgatc cgcagattgg cctgctgcgg
    21601 ctcgaccgcg cggacggcac gccgttggcc atcgtgtatc agttcgcctg tcatccgatc
    21661 atgaacccgc cgagcaaggg caactcggcc gactaccccg gctttgcctc caaagtgatc
    21721 gaggaggcca ccggcgcgat ggcgttcttc gtccaaggct gcggcggcga catcaatccg
    21781 gtgcattaca aggaagtcag ccgccccgcc gatgccgagc cgctcggcac gatgctcggt
    21841 gcgagcgcgc tggccgcgct ccggcgcatc gagccgcagg ccgacgccac gctgagggtc
    21901 agccgcgagg tcatcgccct accgcgcgcg gccgactacg aggcgcgcat caccgccatc
    21961 gagacggagc aaaaggccct cgtggcggcc cttaagccca cgaacatcaa tttcaagacc
    22021 ttcctgccgc tgctcctgca gcaccggctt ttcccggaca tgccatcgca ttacgcgcag
    22081 ggctacctcg acaacccgga gccgctccgg cagctcgacg ccgacaacaa ggcgctcgtc
    22141 gaggcctacc tcggcaatct cgccctcatg gaaaagctca cgcggctgaa cgcgaacctc
    22201 gccctgctca aaaagcacct cgccgacaca aaggccgcga acagccccac gctcgacgcc
    22261 gaggtctgcg gcctgcgcgt cggagccttc aagctcgtca cctttcccgg cgaggtcacc
    22321 gtgcaggtcg gcctgaacat caaaaaagcc gcgcaggatg ccaacgcgca cgtctccggc
    22381 tacacgaacg gctacatctg gtacaccgcc accgagcagc aacggcggaa caccggctac
    22441 gcgcaggagg attgcgatgt gatggttgct ccggagtggc aggccatctt tgaagccaaa
    22501 gccctgcaag tgctgcgcga gctggcctgc tgagcgccgg ccggcaaagg aactcacggt
    22561 gttcgcatgg tgagatgggg ggggttcttt cccagggcgg gaattttgaa aaagggtggc
    22621 gcacatcaag aaaagagttc ccgcattttg aaaaaccacc ggcaaatctt gagaagcaga
    22681 gagtgaaaaa tgcaaacacg gccctgcgcg cccccccgcc ctggcgacac cacattctgg
    22741 cgaatgcgcc tacagggcgc ctctcccgtc ttcgtgtgat gggcaagccc ttcgggctag
    22801 atccagcgct gcaaagtcac cgaatctcga gcacggggga aacgcatgcc ttcctccccg
    22861 cctccacatc acgcgccgca gccgatggcg caggcgggcg caagctcgcg gacttcgtcg
    22921 gcggcggggg cggtcatgcc ggggttgggc tcctgggggg cgaggccgag ttctttgagc
    22981 agggccatgt cctcattggc gcgcgggttg gcggcggtga ggagtttgtc gccgtagaag
    23041 atggagttgg cgccggcgaa gaaggccagg gcctgggcct cgcggttgag gctggtgcgt
    23101 ccggcgctga ggcggacttt ggcgcgcggg atggcgatgc gcgtgacggc gatcatgcgg
    23161 atgagggaga aggaatccac ctggacgttt tcgccgaggg gggtgccttt catgggcatg
    23221 agggcgttga cgggcacgct ctcgggatga gggttgaagt tgctgagcac ttcgagcatt
    23281 ttcaggcggt cctcgatggt ctcgccaagg ccgaggatgc cgccgcagca gacggacatg
    23341 ccggcgtcct gcgcgtggcg gatggtttcg agccggtcgt cgaaggtgtg ggtgctgacg
    23401 atgttcgggt agtgctccgg gctggtgtca atgttgtggt tgtaggcggt gacgccggct
    23461 tgcttgagct gggcggcctc ttcggcgccg aggtggccga gggtgacgca gacctccatg
    23521 ctgagggtgg agacctcgcg gacgatgtcg agcacctggt cgaacttccg ggtgccggcg
    23581 cgcacgcctt tccaggcggc gcccatgcag aagcgtgtgg agccgctggc gcgggcggcg
    23641 cgggcgcgct ccatgacctg gtctttttcc atgagcttct cggcctggac gccggtgctg
    23701 tagcgggcgc tctgggcgca gtagccgcag tcctcggagc agccgccggt cttgatgctg
    23761 agcagggtgc agagctgcac ttcattgccg gtccagtgct cctggtgcac ggcgcgcgcc
    23821 tgctggatga ggtcgaagaa gggctggtgg tagatgcggt ggaggtcggc gtatttcatg
    23881 aaaacagggg attattgagt ccagcggctg ccggggctga gggcgacctg ccagttgcgc
    23941 tggtgcatgt gggagtagtc ggttttgccg atggcgccga tcatgggagc gccggtggcg
    24001 cgtttccagg ctttgctcat gctttcgccg gtgtggcagc cccagctttt gcagaaggcg
    24061 ccgcgggcga aggcccagcg gttgagccgg cgcaggtcgt tctcatgcag ccagacggcg
    24121 gaggcggcgt aaacgtcgga gctgtagtcg aagaggaagc agaacttgtt cgagtggccg
    24181 aagaactcga agccggagat tttgtgccgg ttgcgggcga tctggccgcc gccctggttg
    24241 atgtagttga tgacttcctc gccggtgcgg aaccagacga gattgatctt gtaggtgtcg
    24301 cgcacggact cgacgtggct ggtgagcggg tcgcggtcct cggcggcgcg gcgcacatag
    24361 ccgtcgcgga agacgagcca ggtgacgagg gtgccctggg gctgggtgcg ctggatttcc
    24421 tgcatgcgca cgcgggcggt gcggacgaag ttcccccacc agcggtcgtg ctgctgctcg
    24481 gcgcggcgca gatcctccca cttgcgcagg gcggggccgc cggagcagat gatgtattcg
    24541 ttctgcgcca gggccggcag gctggtgagg agaagagcgg agaaaatgag cgcgccaggc
    24601 aggttcatgc ggaggcgggt ttttaacgcg cgggcgccgg attgtccatt gctttgaagc
    24661 cgcagggcgg cggcgttttc acgcggcgcg gcgctttcct tgggaaatcc tgcgcggctt
    24721 tgggaaatct ctgttcgcgc gttttgaata tggtatcctc ggcggcggtc acagcccctt
    24781 tcccagccat gaaaacttca tttggtcttt cccttctcgc ggcgctttcc ctcgccgcca
    24841 cctgcgcttc cggccagtcc gccaagtcac cccccgcggc gcgtccggtg ctgatgccgg
    24901 agctgggcat ccagccggcg gtgctctcgc cggactcgac gatcgaggtg gtcttttcga
    24961 cgccgatggt tggcaaggag ctcgtcggca cgaaggcgga gaattctccc ctgatcgtga
    25021 agcccgcgct ggcgggcggg ttccaatggg tgagcagccg cagcgggcac ttcgtgctgg
    25081 agcaggcgcc gaggttcggc acggagtatg agttttccgt gcgggacggg ctgcgggacg
    25141 agtccggcgc cctggtgcct gcaggcgtgc tgggcacggc ttcgagcgcg ggcttccagg
    25201 tgagggacca gtcgccgcgc tggttttatg acgagatggc gctgcgcctg ccgaaggtga
    25261 tgctggagtt caacgacgag gtgagcgcgg ccgaggcggc gcgcctggcg gcctttgtgt
    25321 caggggagcc ggcgctgcgc gtcgccgcca aagtgcggca tgcgaagggc gcggatttca
    25381 cccgctacaa cgagccggac ccgacctggg cggaggagat cgcgggcgtg aaacctgtgc
    25441 tcgctccgga ggcgacacgg ctgagcgcgc tggtgatcga gccggcggag ccgctgccgg
    25501 tggcggatga ctggacgctg cacctgccgg agactttgcc caacgcctcc ggccatgcca
    25561 cgctggccgg ggcgcaggtc atcgagctcg gctcggtgaa gccgctgatg gtggtcgagg
    25621 cgcgggggca cacgcctttt gacagcccgt attatgtgga tgcggacttc aacaagatgc
    25681 tgctgccgga gcgcgaggag gagtggaagg cggaggaaat ccaggccctg gcggagaagc
    25741 tggccgcctt cgtgcatgtg acgcccgagg tggcgggcct gaaggcgacg gtgtttgact
    25801 ccacactgcg gttgagcggc cccttcgcgc tgaacacgcc ctatgaagtc actgtggccc
    25861 ccggcatcct ggcggcggat ggactgacgc tggaggaagc ctccgtgaac gaggtcgtct
    25921 tcatcccgaa tccgcctcat gtcgccgcgc cggccttcgt gcgggcgcag ttggccaaag
    25981 gcggcgcgga gtacggcatc gtggccgcca atgtctccag cgtgcgtgtg cgcgccaggc
    26041 gcctcaccgg ccccgagctg ctgcaggcgg tggcgaagtt ccgcgactac gagagctttt
    26101 accacaagga cgaggaggac gaaaaacgcg ccgccttcaa gcccgcgccg ctcgacgaat
    26161 accccggcgc gctggtcttc gaccgcagct tccagatcaa caagcccctg gatcaaagcg
    26221 agatcatcaa gctcaactgg cgcgaggtgc tcggagacaa cacggccgcc ccgctttttc
    26281 tggaatgcga aggccgcgcc atggaggggc tgggagcgcg cggcgtgctg acacagacgc
    26341 tcgtgcagtt caccgacatc ggcctcatgc agaaaagcaa cggccgggag acgctcgtct
    26401 tcgccacctc cctgcagacc ggccagccca tgcccggcgc gcgcttgacg ctggtggact
    26461 ccgagatgcg gctcatgggc tacgccgaca ccgacgcctc cggcctggcc gtggtgaagg
    26521 gagaaaaccc cgcccacatc ctcgccgaga aagccggcga ctgcgccgtc atccgctgcg
    26581 aggactcgca catcggccat gtcattcctt atgacatccc caccgcgtgg gaaaacgtct
    26641 ggaagcccca aatccagact ttcgtcttct ccgaccgccc gctctaccgc ccgggtgaca
    26701 ccatgcacat caaggcgctt tgccgcgtcc gcaccgctga cgaactggcg ctgccggcgg
    26761 aaggcatgaa ggcgagcgtc acgctgcgcg acccgcgcta ccgcgtggtg ctgcaaaagg
    26821 aggtcgtgct cagcgcggga ggctcatggt cggatgacat caagttgccg cccggtccgc
    26881 tgggcgccta taatttgaaa atcaaccccg tctcagagga tgacgactcc ggcagctccg
    26941 gcggccatct gtatttccgc atcgaggact acaagccgaa caccttcgag gtggccttga
    27001 aaaaggatga actcgaaatt cagcccgacc gcctgcgcct gccgctgacg gcgaagtact
    27061 tcatgggcaa ggccctcacc cgctccaagg tggagtggac cgcctactcc ctgcgccagt
    27121 tctccccgcc ggagccgtgg tctcagtttc acttcggcga cgcgccctcc tgggctcgct
    27181 atggcaagga ccgcgacgcg gacggctact acgatgacag tgactcggat gaggaaagcg
    27241 agtggtgggt ggatggcacc gcctatctcg acgatgaagg ccgcgctgtg ctggaaatgc
    27301 ccgtgccgcc gccggaccgc gccgcattgc cccagcaggt gcgcgtcacc gctgaagtca
    27361 ccgacatcaa ccagcagacc atcagcgcct ccgctgaggt ggaggtgccc ggcgcggatt
    27421 tcctgctcgg cctcaaaggc cccgatttct tcggcaccgc aggcgctgag acgcaggtgg
    27481 agatcgtcgc cgtggactcg cgaggccggc cagtggcggg cggcattcgc gcggaggttc
    27541 tggtggaacg ccaggaatac cacaccctga aaattgccac tgcgggaggc ggcaccacaa
    27601 cgaaggatca agtcatcctg cgcgaggagc tcaagcagtc cgtgccgctc caggccgcca
    27661 ctccgggcag cgctcccgcg gcggtcatcc gcttcaagcc cgcgcgcggc ggcgcctact
    27721 tcgttactgc caccacccag gacgcccagg gccggaaggt gctctcccgg atgccgcttt
    27781 acgttatcgg cggcggcgaa ttcccgtggg ctatggagga cggcgcgcgc atcaatctcc
    27841 aaccggagaa gaagactctc aagccgggcg aggaggcggt catcgtggtg aagaccccca
    27901 tcgccggcac cgctctcgtc agtgtggaga ggaacaaagt ccaccgccac ttcgtcacgc
    27961 aaatctcgcc cgaccagccc gtggtgcgtg tgcccatcca ggaggaggag gcgccgaatg
    28021 tctttgtctc cgtgctcgtc atccggggca gtgaggccag tccgaagcag cacaaaatgc
    28081 ccgaatacaa gctcggctac tgtgagctgc tcgtggactc gaaggcaaag gttcttgccg
    28141 tggacatcca gcccgcgtca gaggaagtcc tgcccggcgg cgagttgccc gtctccctca
    28201 ccgtcagcga tgccaaaggc gcgcccgtgg ccggagccga ggttgcgctt tatgccagcg
    28261 acgagggcgt gctcagtctc gtcagccacc agacgccgga tccctccgcc tatttccacg
    28321 agcagcgccc gctggccgtg gacaaccaca cctccttcga cgacctgctc gacgaggact
    28381 cctccgcgcg cagccgtggc aacaagggct tcctcatcgg cggcggcggc gaggagggca
    28441 ttgctgatgt gctcgtgcgt aaaaacttcg tcgccacacc gctttggatc gccaccgccc
    28501 tcaccgacgc ccagggccgc gtcagcacca cggtcaaagc gcccgacaat ctcacccgct
    28561 accgtctcat ggccgtcgtc acccatggcg cggaccgctt cggccacggc gaggccgctt
    28621 tcaaagtcaa caagccgctc atgatcgagc ccgtcgtgcc gcgctttgcc cgtctggatg
    28681 acgaagtgct gctcaaggcc gtcgtccaca acaccactgc gcatgagggc gaggtggaag
    28741 tgcgcctcga gctggacgaa accgccgact tcatccgtga cgagcgcctt ttcatccccg
    28801 tcagcctcgc gcggaaaccc gccgagggcg aaaaaagctg gacgcaaacc gtgaccctca
    28861 aagctcatga aaccgccgcc gtcgccttcc cggctcgttt tgtgaagctc ggctccgcgg
    28921 attggaagtg gaccgcccgc acggtgaagt ggagtcctgg agcgcccgcc ctctccgacg
    28981 gttccgtctc caccctcgaa gtccgccacc ccgtgcccga actcaaggaa gtgcgttacg
    29041 cgcgcgtcag cgccgccggg ccgcccgccg acctcgccgc caaaatcaac cccgcccttc
    29101 tggagggtga gggcgcggtc cgggtggctc tgagcaccac ccgcctcttc gaggtccagg
    29161 acgctttgga ctacctcctc agctatccct acggctgcgc cgagcagacc acctccgcca
    29221 ccatgccctg gctggcattg gggggttatc acacactctt ccccgcccac ctcagcgccg
    29281 acaaagcccg cgccgccatc caaaacggtg tggaccgcct cctgcaaatg gtcaccgacc
    29341 gcggcggcct tgcctactgg ccgggtggca gcgaaccctc cctttgggcc agcgcctacg
    29401 gcggcttcgt gctcctgcgc gcccgctccg caggcgccct cgtgccggag gagacagtca
    29461 acgacctcgt ccaatacctc gtcaaaagcc tgcgcggcct ggaaaaagag gccgacttct
    29521 accgcgtcac cgatgccgcg ctcgccctct acaccctcgc gcgcgccggg cacccgcagg
    29581 agtcttatca aaacctgctt catgcccggc gcgatcaact gcccgagacc gcccgccttt
    29641 acaccgcgct ggccatgtgc ctgtgcaact cccccgccgc gcagatcaag gacatgctcg
    29701 ccgcgcccgc tgtcaaaaaa ggcgccaagc ccctgcccgc ctggagttac tgggccggca
    29761 gcggtgtcaa ccccgccctg cgcctcatcg tctgcaccca cctcggtttg aagaaggacg
    29821 ccgaggacct cgcgctctcc atcctcaact cccgcaacgg ccgcggcgaa tgggggaaca
    29881 cgtttaccaa tgcctggaca ctccacgctc tcaccgccta cgagcgcagc ctcaagccct
    29941 ccgcggagcc gctcctggcc cgtgtccaat ggggcgcgcg cgaagccgaa atcaatgtgc
    30001 cgccccaccc aggcagcgcg caggtttccc tgccgctgga cgccaggctc accgccgcac
    30061 ccatgcgcct cactcttccg gagcagcgcc agggcttcac acgcgtcgaa gcccgcgcct
    30121 tcccgcccgc gcgcgatttc cagggcgaga acaaaggcta cgccatcacc cgcagctacg
    30181 ccaagctgct cgaccgcggc gagctcggag gcgcggatga cctgcgtgtc ggtgacatgg
    30241 tcgttgtcac attgtggatc gagatcggcc agggcgcgca ccgttacctg gccatagacg
    30301 acgccctgcc cggcgtgctt gaggccatca accccaactt cgacacgcag aaccagcgcc
    30361 agggcgcgca gctcccgccc ggcatccagg cctggcactg cgaccaccgc gagatccgcg
    30421 ccgaccgcgc cctcttcttc accaattacg ccccgcgcgg cggcaagttc cagctccgct
    30481 atctcgcccg cgtcatcgcc gagggcgaca ccaccgcccc gcccgcgcgc attgaggcca
    30541 tgtacgagcc gcatcgttac ggactcagcc ccgcgcagcg cctcaagaca ctgccctcaa
    30601 gcggcggcca ggtggccggc cggtaatggg gctgccctcg caagcggacg gtgatttcac
    30661 atgcttaatt tttgattgtg ttttcggccc tttgcttgag cggtgcgccc cagtgattct
    30721 tgtcctgaca gcaggttttg gcgacggcca taacaccgcc gcgtcttcca cagccggcgc
    30781 ggtgcggcgt cttcatcccg gtgaagaagt tgtcacatgc gatctggtgt ccgaggcatt
    30841 gccggcagtg gccgccgttt taaaatatct ctaccagcag gcgatcactc acatgccctc
    30901 agcctggcgt ttggtctatc ggatgctgga gaagtcggac tttgaccctg acaaacaacc
    30961 gtggctgctg ccgttggtcc gtcacttgcg ggacaagatc gaagcgttga agccgcgggt
    31021 gatcatcagc acctaccccg tctatgcggc gctccttcaa gtgcttcgcc atcaaggcgt
    31081 gagcccgcct cccgtggtga ccatcatcac cgactcggtc agcatccacc gcgtctggct
    31141 catgcagccc agcgatcttt attgtgtggc tgacgaggag tcgagatcag tggtgattgg
    31201 catgggggtg ccggcggaca agattcgagt cacgggtttt cccgtcagtc tgcagttcat
    31261 cgagccgctg ccgggagagg cggagaagcg tgcggacaat gggcggattt tatacctgcc
    31321 ctcgactccg gcgcggcatg tggctgccac cttgcgggag ctaaggccgt tgctggaaaa
    31381 tggcgcgcgg ctgactctgc ccgcgggcaa acacaagcag cggctgtacc atgtgctgcg
    31441 ccgcttcacc gacgcgcatc ccggcgtgca gctggacatc atcggctgga ccacgcgcat
    31501 tccagagttg ctgcgcacgc atgatgtggt gatctgcaaa gccggaggcg ccatcatgca
    31561 cgaagtgctc gcggcgcggg ttccggctgt cattgattat gtggtgccgg gccaggagga
    31621 ggggaatgcg gagatgctgc tctcccgccg ctgtggcctg cgcagccact cgccgcgcga
    31681 gacggcgaag gcggtggaat caatcctggc ggaggactgc cgcctgggcc gcgagatgag
    31741 cgcgaacatg gcccccatca gcgtgccgga tgcggccatg cgcaccgcgc gcgcggcgtt
    31801 ggaagccgcc actcccgggc ttgcgcgaga cgcgggctga atgatagcgc gcttgtcacg
    31861 cgggcagtga gcgcttaatc tcagctcatg attgacctgc tgaaccagcc cggcttccag
    31921 aagcgcgtga tcccgctgtc tgtgaaggcg tggcatcaaa tgattgccaa gggcctggcg
    31981 cccgagcgcg cggaactcat ccggggggtg attgtcgaaa aaatgcccaa gtccattctt
    32041 cacatcaaac tggctggccg ccttttcatc cttttgcttg aggctttggg aggcagcttt
    32101 tgggtccgca aagaagaccc tctttctctg gaagattccg agcccgaacc cgacttatcc
    32161 gtgattgagg ggcgggagga ggaattcaac agccacccct ccaccgccaa gctcgtggtc
    32221 gaggtttccg tctccactct ggccgaggat cgcgccctgg ctcccattta tgcggaggcg
    32281 ggtgtggagg agtattggat catcaacgga cgcgagcgct gcctcgaagt cttccgcaag
    32341 ccgggaacct ccggctacga aattcaggaa acccactccg ccgggcagac gctgctttgc
    32401 gcggcgctgc ccggggtttc ggtggatgtg agcgctcttt tccaggggct gcctgaagcg
    32461 gaggcctgag cgcgctcacg cgaacaaatg cgtgagcggc tggcctttgt cggcgacggt
    32521 gaaggggcgc ttgctgggcg agtagatgac ctggcacagg ggcaggccga gcgcgtagcc
    32581 gatggtggcg ttgaggtcgg gcgggccgac tttgtccgcg gcgatctgcg cgccgtcctt
    32641 gtcggtggcg ccgtatttga tgccgccctt gatgccgccc ccgaagatga cggaggaaaa
    32701 gatggggtaa tggtcacgac cctgattttg attgatcttc ggcgtgcggc caaactcgga
    32761 ggcaagcacg atcatggtct gctggagcag gccgcgcgag tcgagatcct gcacgagcgc
    32821 gctgagggcg gtgtcgagcg tttcgcagag gcgcggggtt gagatgaagt tggcggtgtg
    32881 ggtgtcccag cttccgaggg agacctcgat gaagcgcacg ccgtgctcga cgaggcggcg
    32941 cgcgagcagg cagccctggc cgaaggtttc cctgccgtat ttcgcgcgca ggtcggcggg
    33001 ttccttggtg agatcgaagg ccacgagatc ctcgctcttc atcatgcgca cggcgtcgtc
    33061 atacatgtcg gcgtaggcct tgacgccggg cacgtcccag cggttttgga attccgcgtc
    33121 gagcgcctgg gccatgtccc gccgcatggc gaagcggtca tcggtgacgc ctttttgcgg
    33181 gcgcacattt tgaaggccgg tctcggggtt gttgatcatc aaaggcccgt gcttcctggc
    33241 catgtaaccc gcgccggggt ggcggctgtc attgccgatg aagacgtagg gcggcaggtc
    33301 ggggtttccc ggtccctggt aaaagctcag ccacgagccg agaccgggat gcttgatggt
    33361 gctgcgcagg ccgtagctgg tgtgcatgaa atagttcgcc tgctcgtgcg cggcggtgtt
    33421 cgtggccatg gagttgatga cgcatgcctg gtgcatgagc ctggcagtgc gcggcaggta
    33481 ttcgccgatg agcacgccgt ccgcgctggt cttgatggcc ttcgtggcgc ccatgacctc
    33541 ggtgttgccg ggtttcgggt cccaggtgtc gagatggctt tgtccgccgg tcatgtagag
    33601 aaagatgacg ttgcgcgccg tgggcgcctg catggcggtg gccgcgccct cgcctgcggc
    33661 gagagctttg gaggagaaca agtgctccaa ggagggcagc aggccgacac cgaggcagga
    33721 ctgcgccatg cgtgtcatga cggtgcggcg cgagagttcg ttcgtggctt tcatggctgg
    33781 aatgggctgc gtggtttcgc gggttactcg atgaagatga agcgctgggt gctgacggcg
    33841 gcgagggcga ggtcctccca cacggtcttg ctgccggcgt agctttccat ccgctggcgg
    33901 atgagggcgc gctcgctgtc cgagggcagg cgggagtaga ggctgaggaa aagcacgtcg
    33961 agcttgtcgt ccggcgtttt cgccttgcgc aggttcagca tgagggcgga ccaaccgctg
    34021 gtgatctggc tgccgatgga gccgttcatg acgagcaggg cttggggcac ggaggcttcg
    34081 tcgctggcgt tttcgatgac ctcgcggtcg ctctggccga actcgcgcag gaagtgcccg
    34141 cgcggcgccg gggagggcag ctcggcggcg cgcacccagg tctggtggag tgtttttaga
    34201 taaccctcat agcctttgcg gcttttttca tccttgtaac cgagcatctc ggcatccttg
    34261 agaaggcgct ccttgagcgc gccctcgaga tcgcgctcgc gcacttccag gcgaggcgtc
    34321 tccatggcat tcatcatggc catttccatc tcaccgcccc cgctcatctc ggcgagcatt
    34381 tgtttgactt cggcattgcc ggatttctcg gcggcggcat ggaagcagcg ggaaacggtc
    34441 tcgcgcagga tgcgctgact ctggccgagc cggcgctgga tttcgctgat cttggccttg
    34501 tcctccttgg cgcgggcctc gtcgagttct ttgcgaagct ggtcaaactc cttgttctga
    34561 acacgcatgg cgccggccac ttccttcgcg gcctggtaga gcagggcggg ctcggttttg
    34621 tcgagcatcc gggccagcag gtggcgcttc tcggattcga cgcgctcgcg ctcgcgggcg
    34681 gaccagttgg actggtcggg cacggggctg atgagcgcga tcatggagtc ccacgcctgc
    34741 tcggcgctca tgcggcggaa gaccggaccc tgaaaataac aaggctctcc catgccgacc
    34801 tccttgatgc tttcgcgggc aaaggtctgc gtgttgagca gcatgcgcag gtaagccttc
    34861 atgtcgtagc ccagcgccac catctgctgc tcgagatatt tcatcagctc ggggttcgat
    34921 gccgtgctgc tgtccatcag gtcgtccaag ggctcaatga gcgcgaggcc gaagaccttt
    34981 ttccagagcc ggttggcgat gagattggtg aatcgcggat tctccggcga ggtcagccat
    35041 tcggcgaagg ccttgctgtg gtcggtctcc ttgccgatct ccaccggctt gccaaacatg
    35101 accgcggccg gcaccgcgtc cttgggtttg gcgtcggagt atttatagtc gtgcggaagg
    35161 cggatggcgg ctttgttctg aaccacctgg gtgtcgcgca gcgggcgcac gacctcggtg
    35221 atggcctggc gcatgaggtc ctgagtctcc ttgtcgagcg tcttgtccgc gcggatcatc
    35281 ttgttggtct cgtcggcggt ggtgctgcgg tagctgccgg aggtcatgtt gtgcgtgaag
    35341 gcggccatct cgtagaactg cttctgggtc cacttgtcga aggggtggtc atggcattgg
    35401 gcgcactcca tgcgggtgcc gagaaagacg cgcgtggtgt tggagaggtt gtccagcggc
    35461 atgccgcggt cccgcatgta gtagccgatg gcgccggtgt cgaagcaggt gcccgcgctg
    35521 gagacgagct cgcgcgcgag ctggtcgtag ggtttgttgg tcttcagcga gtcgcgcagg
    35581 aagttaaggt aattctgggc ggtggtgctg cctgccacac cggcgctctg ggcgcgcagc
    35641 acgtcggacc agtagttgag gaagtgatgt gtgtaaccct ccgactccag cagggcgtcc
    35701 acaagctggg cgcgcttgtt gggcgcctcg gatttcaaaa aggcggcggc ctccccatat
    35761 gtcggaatgc ggcccaccac ggtgagatag gcacggcgca ggaagacctc gtcgcccacg
    35821 ggcgcgttgg gctggagttt ctgcttccgc cacccggcgg cgagaagctc gtcaattttc
    35881 ttcgaggctg ccgaggtgtc ggcggcatgg aggtggggcg tcgtggtcag gcagagcacg
    35941 gcgaaagcgg atgtcaggag ggctttcatg cggaccacca acgggaatgc caaaccggag
    36001 gtgtcagcgg cggcccatct tcacaaatga ggcggcagac atgacaaaat ctggcaagcg
    36061 gagcctgagc gcacggtttc ggagagacgg gaaaatccaa aaatgcaaaa atgccgcgaa
    36121 cgccccggag gcgggccggg gcggccggaa cggcggcatt tgttcccttg catttgccgt
    36181 gaacgtgcct aaaacgcacc gctctatgcc atccacccca gtcatcaacc tccgcaaagg
    36241 ccacgccgtt cgttacaaca acgaggtgtg cctcgtctcc gactttgagt tgaagacgcc
    36301 cccgcgcatg gcttcctatg tgcagatgtc cgtccgcacc ctgagcaccg gcaaagtgta
    36361 caacctgcgc atgacctcca acgagtcgct cgaatccgtg aacctcaacc gcgagccgca
    36421 cgagttcagc tacaaggatg gtgacgacta ccatttcatg caccccgaga ccttcgagga
    36481 tgtcatcctg atgggcgacc aggtgcgcga cgcgaagaac tacctcatcg aaggccagaa
    36541 gtacacggtg cagttcgccg acgacattgt catcggcatc gagctgccgc cctctgtcat
    36601 catgaccgtg accgaggcgc ccgaaggcgt caaaggcgac tccgccaaca acgtctataa
    36661 agccgccatc ctcgaaaccg gcctgcaggt gcaggtcccg ctcttcatcg gccccggcga
    36721 caagatcagc atcaaaaccg aggacaacag ctacctcggc cgcgccaact gaatttcgga
    36781 gcaaactctt ccgcgaaagc cccggcatgg cagccggggc ttttttgtga tgagtggaaa
    36841 ctcctggctc atgcgccggc agcgcccagc cccgcatagt agggggcgat gcggcgcgcg
    36901 tgctcgtcat aggcggcttc gaaaag
//
LOCUS       DAOMYJ010000028        35151 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Prosthecobacter sp. isolate AMR_MDS_5629
            NODE_446_length_35151_cov_21.914007, whole genome shotgun sequence.
ACCESSION   DAOMYJ010000028 DAOMYJ010000000
VERSION     DAOMYJ010000028.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867850
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prosthecobacter sp. (wastewater metagenome)
  ORGANISM  Prosthecobacter sp.
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Verrucomicrobiaceae; Prosthecobacter.
REFERENCE   1  (bases 1 to 35151)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 35151)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.30
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:34:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,742
            CDSs (total)                      :: 4,684
            Genes (coding)                    :: 4,676
            CDSs (with protein)               :: 4,676
            Genes (RNA)                       :: 58
            tRNAs                             :: 55
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35151
                     /organism="Prosthecobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_446_length_35151_cov_21.914007"
                     /isolate="AMR_MDS_5629"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1965333"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..719
                     /locus_tag="PLP58_04840"
     CDS             <1..719
                     /locus_tag="PLP58_04840"
                     /inference="COORDINATES: protein motif:HMM:NF024240.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HRJ07872.1"
                     /translation="CPGWLLPRAARLYRPAYVCAFHHVSGGQDDTWRLNLGGRIVRAV
                     QEGRLPAAALGAGFRASPFKGEVFLTVDLEAGQDEWDPLDPAKRAGVEAEGRTVALAL
                     WRELRAAHPDFAQCPPPVLPAQAGIRESDCYAGDHVLTGDELAACARFDDEVALAGWP
                     MEKRENARGPKFRFFERAEPAGIPARCLRRSDAPGLFFAGRCLSADHEALASVRVMGT
                     CMATGEAAGRMAAEHACLQR"
     gene            complement(761..2305)
                     /locus_tag="PLP58_04845"
     CDS             complement(761..2305)
                     /locus_tag="PLP58_04845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ07873.1"
                     /translation="MAKKKQDSSELNLDSLMDAVTNVVGVLMIVFVMMAINTAKMVQK
                     ILTDLPPVTEEQHQQMVQQIKELPPPPADPEKIEEQKKKAQQDLKKVVEELKTIDTSE
                     MAAQMKFMDLDTFRKQLEEAKKTREKEKGEVDKLITEIERLKALLDETPVYQPPPPTY
                     VRLPNPRPYPEKPNETRILVARQGVVHFNQDAFLKPILDGLDKARNQLIYRDAKIEPF
                     ADMLGKIYGTPAAAQQAWAEIAPFVGTFQMENVAKAHKALAAADMPATRQVLTALGDI
                     SIVVRSNMDAVAEAMVAAATGDLTKWTALDPSRDPTKPTIQAVAGEGRITFTWGSQAV
                     DVKNNTRDIQSYFIKDLAGLDSIKGKSKDKVIYDAFAIRSMLERAATNPTFSGPFTLV
                     PAIRPGATLVQMQLTPKAGAGETLDQMRNEGSSYQRMLRALKADPNGVALFQVMNNAF
                     DTYLEARKIADETGVPASWEFLAGLDLTLNVTGYEVQRFALVPGAARGKGDPGIRIGA
                     PKRTLD"
     gene            complement(2318..3208)
                     /locus_tag="PLP58_04850"
     CDS             complement(2318..3208)
                     /locus_tag="PLP58_04850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ07874.1"
                     /translation="MAQRRASSENEVSLFPFLSILACLIGALVLIIVVLVVAQAGKAE
                     GRTAEEIRMAQDYQRMQKEIEERKELDLILKEKLAELEKLQEEVKEKEQRYIKLRKLL
                     DSSKEIKEQNLLISQQLQKELDSLLVEIDGLKKQQTESKTEIEKLLAEIKARQVPEDK
                     KVPPVVVQPSGSGMAESTRVYFVECSAGALKILGAWGEDYRLGATAEIVVADAAYNHF
                     LFEVAKDKNSLILFLIRDDGQGPFNNGAGRASNDYKIRVGKLPIPGRGQLDLALFDKY
                     RGKIDPPVPGAPVPEPAANP"
     gene            complement(3230..4630)
                     /locus_tag="PLP58_04855"
     CDS             complement(3230..4630)
                     /locus_tag="PLP58_04855"
                     /inference="COORDINATES: protein motif:HMM:NF013760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HRJ07875.1"
                     /translation="MQLKAEPEMAGKIVRCPGCNTKLSIPATIGAPPPPSGLPPPPDG
                     VPATASPPPQDASAPGEQPGSHQEYQESYQQQSARGGWEEADPANANIFAALGIGLVL
                     TLTWYGILYPFGVGKYGDPAASTLDYIHDLFYERSWVNMLETFFFFFAIAILWLKGKK
                     LRHQKDAMYLDVLPTDFGQEINGQNVGSFVDHLYGLPGRLRDSMMVNRIRKGLELFEV
                     RQNNSEVNTMLQAQSNIDSARIGGSYSLVKVFLWAIPILGFIGTVLGLSQAIGSIDLS
                     DTSDLDKIMGSIGNVTSGLGTAFDTTLLGLVLALFLNFPISALAKSEDDNLNNIDAFC
                     NEVLLPRLNDGGGVAGGDTGGLMDTLVKAVANAQKEFLVDLNALSAKIKDYADNLDKR
                     AASHQERVDSEFAMTLNRMREDLTNAVKDSVKTTTEYTRALSTGIQGLNNVLTELGGK
                     QILIHQVKKKGWFSRD"
     gene            complement(4829..5941)
                     /locus_tag="PLP58_04860"
     CDS             complement(4829..5941)
                     /locus_tag="PLP58_04860"
                     /inference="COORDINATES: protein motif:HMM:NF024209.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-rich repeat domain-containing protein"
                     /protein_id="HRJ07876.1"
                     /translation="MKTPALLILLLITLNVSAQDPKPAAAPKAETPKPAPVPAPKAEP
                     AKPAEAKKDEPKPAPAPATPPKPDPAKTAETKKEEPKPAPAPAPAPAPKPEPAKTAGA
                     KKEEPKKAEPPPPPKPVPVFKDKALEDAVRRQVFAKRESKDPLTAEDVANVAIIEGKN
                     MGITDLSGLEKCAALASLTLPGNKITSLAPLKGLARLQFLDLAGNQIADISPLSECKA
                     LQYVELTGNKVVNVAPLGGIQSLTSLYLAGNQIEDATPLFKLPKVWTLYLEGNKVSNL
                     EGIGSMKWLSMLSLKGNKVAGLTPLEPLTDLQFLFLENNQITDIAPLHRMWKKDSDGA
                     REWAPYCQIHLGGNPLGEEAKKHAEELKTAGARLTF"
     gene            6022..6309
                     /locus_tag="PLP58_04865"
     CDS             6022..6309
                     /locus_tag="PLP58_04865"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015925.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative quinol monooxygenase"
                     /protein_id="HRJ07877.1"
                     /translation="MVNVIVILEIDPARVDEFLEVITFNAAESVKEPGCLRFEVSRQF
                     DQPNLFALSESYTDKAAMDAHYQTPHFALWKEKSATGFVLNRWSVKGPVLE"
     gene            6318..7316
                     /gene="moaA"
                     /locus_tag="PLP58_04870"
     CDS             6318..7316
                     /gene="moaA"
                     /locus_tag="PLP58_04870"
                     /EC_number="4.1.99.22"
                     /inference="COORDINATES: protein motif:HMM:TIGR02666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP 3',8-cyclase MoaA"
                     /protein_id="HRJ07878.1"
                     /translation="MEDAYGHRISYLRVSVTDRCNERCRYCMPEEEQAWFPKEEVLGY
                     DEMLRVLRVGARLGIRKVRVTGGEPLTRPGVADFCARVSAIPGIEHLGISTNGTLLAK
                     TEGGLTLAEHLARAGVRAANISLDSLDRAAYQRATSRDLLPRVLEGVEAALAAGIPSL
                     RLNCVLMKNQTEREIPALMEYARSKGALLRFIELMPVSTSDVLRDENFLSAGHALRLV
                     EREHGPLAPLPDFKTNGPATYYQVRATGQKIGFIGAMTNLHFCESCDKLRLTSDGKLR
                     PCLGSWLEFDLRSVLRAGCTDEELEGFILGVVARKPKEHDFRNNYLPNRKMIAIGG"
     gene            complement(7335..7793)
                     /locus_tag="PLP58_04875"
     CDS             complement(7335..7793)
                     /locus_tag="PLP58_04875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ07879.1"
                     /translation="MNAPPPPLPDPRVKDDEHLRLLAIFHWVLAGLSLLGMGFLGLHY
                     GIMRMVFGSPEIWKNAKDGPPPEVIWDVMQWFYLGIGLLILLVGFANLAAGFSLRRKK
                     HHVFCLVIAGLNCLQFPFGTALGVFTFIVLLRDSVRAAFKPGQESAAAEL"
     gene            complement(8014..8730)
                     /locus_tag="PLP58_04880"
     CDS             complement(8014..8730)
                     /locus_tag="PLP58_04880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HRJ07880.1"
                     /translation="MSKQTLRKAHRWLGLLFSASILMAAGSGVIHNVMTRSQPPPPPA
                     LPTGTLDASLIRLGVAEALEKLPGEARAVTAVNVRGIGGSPWYQVYSQNGGAAHYVSA
                     VDGRVDPGQDEAYAAEIATRFLAGAAVRKTAHLTAFDNEYLNIFRILPVYRFDLDDER
                     HTRVYVSTTTGSVTRHTDDGRQLEARIFTNFHKLGFIPDKNMRDLVLTTLTTATCVVA
                     LLGIALFFATMPRKKNRPQA"
     gene            complement(8775..10925)
                     /locus_tag="PLP58_04885"
     CDS             complement(8775..10925)
                     /locus_tag="PLP58_04885"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HRJ07881.1"
                     /translation="MKSLPSFLPLTGLLAFSQLPAQTAAPSETLPQVLVTADAPDPSR
                     TVPKLETRRQELAATTPGGVAVVDAEEYKRGRAATLKDALDFAPGVFIQPRFGAEESR
                     VSIRGSGIQRTFHGRGIKLMQDGSPLNLADGGFDMQAIEPLSARYIEVYRGANALQFG
                     STTLGGAINFVSMTGHEASPFQTRFEMGSFGSYRGQISSGGVSGATDYYASFTYNETD
                     GYRDHSRQDSQRFFANIGQKLGENLETRFYLTYVLTDSQLPGSLTQRQLERAPDQANA
                     ANVRFNQKRDFELFRLANRTTWSDDVHSLSLSTFWSWKDLDHPLISFGPGLLLGPGVI
                     DQLSNDLGLDLRYDSLADLAGHRNHFTLGLGYVYGLTQDNRFANLLGRRGPRTAENQQ
                     QSTNVDLYLQDTLHLADSLALVLGAQGSYANRDFDEQRVFGADNSDRQEYWGFSPKLG
                     LLWDAAPGTQIFFNASRSFEPPSFGELTALGGTPGLIELDAQRGTSIEFGTRGSAGRA
                     RWDVAWYYTWLDNELFALGVPGVSATQTINAGRTIRHGIEASLDFDLVRGVLTSWQDA
                     SPATGKQAGTDERPGDRLFFRQFYLFNDFHFDGDKDFSNNELPGIPRHFYRAELLYEH
                     PCGFYMGPNVEWAPQDYFVDLANTLKADGYALLGFKVGYRATKGLSFFLEARNLTDKT
                     YAATTGVVTAATPASAVFFPGDGRALYGGIEWKW"
     gene            complement(11034..11426)
                     /locus_tag="PLP58_04890"
     CDS             complement(11034..11426)
                     /locus_tag="PLP58_04890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ07882.1"
                     /translation="MFTRPSIARLAVTGALMIALGIPWMLAQSVAWLGMAVTYSLEKG
                     SLVEGLSATFDGEHSCPLCKAVEKSGRETRETMLQKVLKLDAVLAGAPRLVTPAGVTF
                     EHPEWRGCRTTGWEEKRFRPPRSGSCRA"
     gene            complement(11480..11842)
                     /locus_tag="PLP58_04895"
     CDS             complement(11480..11842)
                     /locus_tag="PLP58_04895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ07883.1"
                     /translation="MKQRILITTFAMVFLAGAASARADGVISDIMKKYHKAPQGEDPV
                     CKQVSNGEGTSDQIAELLKSYEAMAKEKPEKGQPSSWEEKCAALIAAVKKLQKDPKDV
                     SDYKKAVNCKACHDPHKG"
     gene            complement(12200..12601)
                     /locus_tag="PLP58_04900"
     CDS             complement(12200..12601)
                     /locus_tag="PLP58_04900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ07884.1"
                     /translation="MLTTVARTLLVAALMISIGLHWAVLQSAAWVGMVVAYSVEKGSV
                     VQGISDTFDGEHPCPLCTAVSEGVKESPAKNGSAPVKPGKDLKITLAIVPVPVFVFTT
                     APPPAWSTISSTAEARQVQPTAPPPRLAAII"
     gene            12707..13687
                     /locus_tag="PLP58_04905"
     CDS             12707..13687
                     /locus_tag="PLP58_04905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009958946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3500 domain-containing protein"
                     /protein_id="HRJ07885.1"
                     /translation="MKNKILSAAPSRRQFILNGAQALAAGWAGSSLAETASMPRSETL
                     VQQLYGSLKEEQRKALCFAYDDPRRLKVDNNWHIVPQRLRDTLGADQQDLVRQIFDGL
                     HSEEYKKEVWRQFDHDNKGDGGFASSSIALFGEPGQGKFQFVLTGRHCTRRCDGDSEA
                     GVAFGGPIFYGHAAKGFYEKPGHEGNAYWFQAKRANEVFQALDGRQRELALLGDARDE
                     AGNKTIKLTGKTKGLEGIPMTELSADQKGLVRLVLADLLKPFRKEDAAETLKLIEAAG
                     FDHLHMAFYKNHDVGEDGVWDVWQIEGPSMVWYFRGDPHVHCWAHIRESA"
     gene            13940..15496
                     /gene="cimA"
                     /locus_tag="PLP58_04910"
     CDS             13940..15496
                     /gene="cimA"
                     /locus_tag="PLP58_04910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018290918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043714 - (R)-citramalate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citramalate synthase"
                     /protein_id="HRJ07886.1"
                     /translation="MSVTIYDTTLRDGTQGTGISFSTLDKIRVAEKLDDFGVHYIEGG
                     WPGSNPKDAAFFQEAAKRTWKNARITAFGMTRRGKMKVEDDPQVRMLLDARTPAVTVV
                     GKTWSLHVTEVFQVSLEENLAMIADTVAYLKKQGREVLYDAEHFFDSYREDPEYSLKT
                     IQAAQDAGADLVVLCETNGGALPEFVEEATRKVIAHLGHPVGIHTHNDGGVGVANALA
                     AVRAGACQVQGTINGYGERVGNCNLTTVMPALQIKMGVPLGMDLTRLRELSFFVDELA
                     NVPHDIRAPYVGLAAFTHKGGLHVHAVQKLARTYEHVDPALVGNERVITISDMSGQSN
                     VLVKAEAMGLPLAKGSKEVQKILTEVKRLESEGYEFEAAEASFELLIRRMLGQETHCF
                     ELLEFHTTHRFRSGMTAETNEATVKLFVHGKAEYTVDEGDGPVNALDKALRKALLPHF
                     PQIASMRLEDYKVRIIDSGSGTAAKTRVLIVSTDGARTWGTVGVSTNIIDASWQALVD
                     SMEYFLLQGR"
     gene            complement(15572..18379)
                     /locus_tag="PLP58_04915"
     CDS             complement(15572..18379)
                     /locus_tag="PLP58_04915"
                     /inference="COORDINATES: protein motif:HMM:NF037085.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBG domain-containing protein"
                     /protein_id="HRJ07887.1"
                     /translation="MKRFPHFAACAALCAAHVTQAATLTVSNYNAAANGFHAISDNAS
                     TRIAPGTGGGVIGRMATLTDEQVNTHVASGNIAALNADFRVFGSPFALDLTSTGPDGA
                     LEKAVSFDTRVSSDPNNFGGNAIYAWLYKGASRTSATEYFLVKLSQNFPTDPEDLPPL
                     GPIEVPVRPGTIATLYAGAAGPGTFDYGFGSGPLTILRMQATGSSGNNAPVAENKTIN
                     VLAGVPFNSSVTATDPDLDSLTFSKVADPIKGDLTFNSNGTFTYTADGGESGQDSFTF
                     KANDGTDDSNTATVTINIQTGGTLAQTISFDAPAQRQVGDAPFDLMATASSGLPVSFQ
                     ILSGPANLAGSTVTLTGAAGVVQVRATQAGDSTFDPAPPVARHFHVVPVSTKFNLINL
                     VQTYTGGPLSVGVTGVPLTGLVITYNGSQNLPVNAGSYNVLATSGTLKKTGKLTINKA
                     PLTITADNQRKLIGQPNPALTFNYSGFLGADDENSVFPPPPAPPAKPTATVPVISTTA
                     KDLSPGGRYPITFKGGLAVNYRFVFVPGTLVVDSFEGRYENLLVSPDTMRAIAKVELT
                     VAKTIKDNKMAFTGKLWTPTDTTALPIKGTLTVDPLTETATGTWTLNKLVNKVAIAYE
                     MTINLDIGGGFEAILDVDGDLFAEADDGARVFVPAKGQIVTNAGLLTLILAPPLPAFG
                     AVNPIPLGSGHATGSVDAKAVMKLTLTLADGIKTTATLMPGVDGGYRLFVNPYKRLDS
                     YLAGWLDLLEHPDLPGRGHIPTMLMGDSLAWAKAAGPKDKSYRGGIDLMDCGITLDPW
                     RKPAKATKTLPEIALLNELGITDSTVISIGHSGFAGVDAGLLPTEVTMAANGKVTVQT
                     PAGNPAVWQITINTATGGFTGKFTLKNEKTRTVNFSGVLRRKLSTNPADFIGRGNFQL
                     PALPSDPTPEIQSGDIQLFVP"
     gene            complement(18413..19441)
                     /locus_tag="PLP58_04920"
     CDS             complement(18413..19441)
                     /locus_tag="PLP58_04920"
                     /inference="COORDINATES: protein motif:HMM:TIGR02597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02597 family protein"
                     /protein_id="HRJ07888.1"
                     /translation="MKKLLNMAIFGVLAATGLLQAQVTSPIMGFLTLHLQEGTNFIGF
                     ALLPTMEMQSSFNISPTDRKIVFLQGGPNLALADNQFNPGPLATHAVEIVSSGAGQGF
                     TTIVTATLSTGNQLTLQEEVPAGVADGASLKVWRLWTLADVFGADNSAGLAGGETPGQ
                     ADLILLPNGTGFDKYFYSTGGAQGVGWRQVDGGTTDQADVPVFVSEGAAIIARAAKSV
                     VVIGQVKPGVTKVSLQTGNNYVANLCPVNAAGEGASVEGRTLGNSGLAEGMAGGTASS
                     LADLVLLWNGSGYDQYFNSTGGPLGAGWRKVGAGSTDQSSVPLPDGAYIILRRGAPVM
                     ITLNQGSF"
     gene            19862..22207
                     /locus_tag="PLP58_04925"
     CDS             19862..22207
                     /locus_tag="PLP58_04925"
                     /inference="COORDINATES: protein motif:HMM:NF012581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proton-conducting transporter membrane subunit"
                     /protein_id="HRJ07889.1"
                     /translation="MIAPWIILSFLLLSALAGSLQKLCPKAAPWLLAAGPGLAALWLL
                     AQMPGVSAGAAPAFSAPWVPELGLELAFRLDGLGLLLGLLVCGIGFFIVLYGSAYFEK
                     EPVARGRFFTLILVFMAAMFGLAVADDLLLFFLFWELTSITSYLLIGFKHKEESAREA
                     AQQALIITAGGGLALLAGLILLGDAAGTLKISELAARAELVKAHALYPWILGLVFLGA
                     ATKSAQWPFHFWLPGAMAAPTPVSAYLHSATMVKAGVFLLARLFPVLGGTELWQNLLG
                     LAGAVTMVTASVLALGQRDLKRLLAYTTVSALGTLVMLLGIGDALSLQAAVVFLLVHA
                     FYKGALFMVAGAVDHATGTRDINLLQGLRHLMPWTAAGAVLAAFSMSGIPPFIGFVAK
                     ELLYEAKVSVPDLRTPLLIAAVFANMATVKVAFSVGLSPFHGAPPEELEHAHDAGWRM
                     KLGFLTLGVLSLVCGLAPQMVNDLLLAPALRALDPEAAVLKLKLWHGFTPVLALSVVT
                     VAGGIAMHLVRRPFQKIRARFAGVAEYGPGALYQWKWSLLLAVADGIQRWIQNGRLRH
                     YLRVIFILTTGLLLLRVLPVLPELARGTELAPRGFETTVVVTMALAGLVAALTRSRIT
                     ALLGLGWVGLGVTYIFVDFGAPDLALTLILVEALTVVLFAVVITRLPPIRPRDPLSGR
                     LRDAGIALLAGAAAGMVVWKAAHVRLHESISPEFMARSLSEANGRNVVNVILVDFRAL
                     DTLGEIMVLGVAALGVYALVRMRSAREDELGADKQEKEDVS"
     gene            22204..22647
                     /locus_tag="PLP58_04930"
     CDS             22204..22647
                     /locus_tag="PLP58_04930"
                     /inference="COORDINATES: protein motif:HMM:NF015970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MnhB domain-containing protein"
                     /protein_id="HRJ07890.1"
                     /translation="MTSRILRTAARLISPMLVALAVILMLRGHNEPGGGFIGGLVLAM
                     ALLLPAFVEQETPRLNLWVDPQQLIGLGWLLALASALLPLLVPGHGFFQALWGGSIWL
                     PVVGKVLFGTPLLFDTGVFLTVAGVTLKIASLFMQAAGNEGRGGL"
     gene            22731..24407
                     /locus_tag="PLP58_04935"
     CDS             22731..24407
                     /locus_tag="PLP58_04935"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF011445.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative manganese-dependent inorganic
                     diphosphatase"
                     /protein_id="HRJ07891.1"
                     /translation="MSDPVYVIGHRNPDTDAICAAIGYAAYLRQVRQDEVVAACCGEI
                     NARTNWVLRQAGVEAPRLLLDVRPTAAIVCRKEVLTAGPRETFLSVYRKMLEHSFRSI
                     PVVDENGRLLGMPTIQEMAQLFLPSDAAHAAANREVRTSLANMAAALGGRLIGGVESA
                     DAVEDLLLVVAASSVETSRDRARQFPPRQVVLVTGDRPEIHALAIELGVRCLVVTGGF
                     MPWDSLLQQAATKGVCVITAAQDTASASQLIRFSRPIGAALHGDYLSFGSRTPLREII
                     HAVQNSHQPLFPVIDEETGRLEGVFSKSDLVEVPRTRLVLVDHNEFSQAVAGADEAEI
                     LEVIDHHRLSGNLRTREPVRFINEPVGSTSTIVGIMFRMRGLAPNKGTAICLCAGLIS
                     DTLHLTSPTTTGTDREILEWLARIAGVDAARFVKDFFAAGSLLREATPARAIEGDRKV
                     FEENGWRISISQIEEMGLDEFWKKQAVLHDALTALCAAHSLHFACLMVTDITAHHSVL
                     LVAGDTRVEQAIDAGYHQRTPQVFDLPGVVSRKKQLFPYLSSLVGKLTPP"
     gene            complement(24476..24793)
                     /locus_tag="PLP58_04940"
     CDS             complement(24476..24793)
                     /locus_tag="PLP58_04940"
                     /inference="COORDINATES: protein motif:HMM:NF014505.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HRJ07892.1"
                     /translation="MIPKLGEVWMVDMGMTGKIRPAVVVLDDQFPVERSLIVHVPITS
                     QNRGSCLEVSLGHLRFLSPESVANVQGIGSLPKARFERKLGVLPEADMERIRAAMRQA
                     FRL"
     gene            complement(24790..24966)
                     /locus_tag="PLP58_04945"
     CDS             complement(24790..24966)
                     /locus_tag="PLP58_04945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ07893.1"
                     /translation="MSARDAILIEIDSLPESVLQEALHFMRFAARQQQEAEWQDVLPS
                     REVEQEVLDLLDAP"
     gene            25117..26007
                     /locus_tag="PLP58_04950"
     CDS             25117..26007
                     /locus_tag="PLP58_04950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ07894.1"
                     /translation="MPHARLFHSCLLLLAAAPHVQGQQEAPAELRPRWEAGARYVQET
                     LTETVTDLSALGSKPEQGMKVKQTTRIEVRPGGEGGKLARVTFTAMSGEAILDGKRQT
                     FDSSDLVSASPEIRASVGRSLGQSFVLVYDKEDRFVEVRDSSSMLPPTEDGQPKLEGI
                     AEAAEVAELFRRSLEMGLPKTAVRSGDHWTTQEEVRFPSAGAVKVELRAQYEAQVDYL
                     GRPHAKISFNGVMKPSEESAGARPVTLGEGSKLFGQVLFDLGAAMTSFGAFRADLTLD
                     VRGKKVPVRQQVTTRMTREG"
     gene            complement(26031..26450)
                     /locus_tag="PLP58_04955"
     CDS             complement(26031..26450)
                     /locus_tag="PLP58_04955"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(deoxy)nucleoside triphosphate
                     pyrophosphohydrolase"
                     /protein_id="HRJ07895.1"
                     /translation="MQDQNRPPVEVVCAVLRRGGRLLVARRPAGKRLAGCWEFPGGKI
                     EPGETAEAALLRELREELGCAARVLRRGPVILHEYPWCAVRMHSFECALEAGAGEPAA
                     LEHDEIAWIEPAELDRLELAPADVPLAPWARGLFEAG"
     gene            26480..26827
                     /locus_tag="PLP58_04960"
     CDS             26480..26827
                     /locus_tag="PLP58_04960"
                     /inference="COORDINATES: protein motif:HMM:NF013098.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HRJ07896.1"
                     /translation="MRPEKVKFMLLAADMRRAVRFYSETLGFEKVFVSDYWSELRCGD
                     AILALHGGHDGSANPTGLSLQYEDVFDAAARIEKSGGAILEMPNQREGEPILLGRFRD
                     TEGNEAFITQYVG"
     gene            complement(27012..28178)
                     /locus_tag="PLP58_04965"
     CDS             complement(27012..28178)
                     /locus_tag="PLP58_04965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009961211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="KamA family radical SAM protein"
                     /protein_id="HRJ07897.1"
                     /translation="MRTPPTLLPEKEFRSHAPGFWAGRNVPAEDWNSPAWQLRNRVTT
                     LAGLEEHLVLAEEERAGVLLSGRKLAMAVTPHYFNLIDPLDPEDPIRRQVIPRIEETW
                     EDPDEMSDPCGEDSHMPVPGLVHRYPDRVLFLVTDRCAAYCRYCTRSRVVSGVGEQEL
                     HTDHEAAFRYLEKHTEVRDVLLSGGDPLLFSDAKLEAILSRLRAIPHIEFLRIGSRVP
                     IFLPQRITPELCRMLQRHHPLWLSIHTNHPRELTTEVRAALGRLADHGVPLGNQSVLL
                     RGVNDDAEVMKSLVHKLLMCRVRPYYLYQCDLIQGSSHLRASVQAGVEIIEQLRGHTT
                     GYAVPQLVIDAPGGGGKVPVNPDYVLLRDEQRTLIRNFEGRTFEYPEPAMARLR"
     gene            28331..28573
                     /locus_tag="PLP58_04970"
     CDS             28331..28573
                     /locus_tag="PLP58_04970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009962055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043231 - intracellular membrane-bounded
                     organelle [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDGSH iron-sulfur domain-containing protein"
                     /protein_id="HRJ07898.1"
                     /translation="MADLPKIHDKKPAALEIEAGDHWWCACGLSGHQPMCDGAHKGSG
                     MAPKKFTLEEKRKVWLCNCKHTKNPPFCDGSHSSLA"
     gene            complement(28732..28956)
                     /locus_tag="PLP58_04975"
     CDS             complement(28732..28956)
                     /locus_tag="PLP58_04975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ07899.1"
                     /translation="MPYDPAQPANNSALSSAVMRAQLTGLKDLIDAVPVITGAVVDSV
                     TPLPPGDSPTVSVALIDGVLRFSFGIPSAS"
     gene            complement(29087..29725)
                     /locus_tag="PLP58_04980"
     CDS             complement(29087..29725)
                     /locus_tag="PLP58_04980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ07900.1"
                     /translation="MSTTIKAVISFQSYTDGNLGPTAQTIHGKMTLNAGIFDDPPVTM
                     VVFQGQITDYMTKLSARASRATADIMAFNAARELLEETLGDLGGYVNIVAKGDGLIVE
                     KSGFPFYDTDKAPDTTPPNAPADLRLKHSELPGGFIARYKPEKRGSVNEVQTSTGDPN
                     NEADWVQKAIIKGGRAEIMGFAPGTIVWVRVRTVGIKGIMGAWSDPAQIRVL"
     gene            29986..30402
                     /locus_tag="PLP58_04985"
     CDS             29986..30402
                     /locus_tag="PLP58_04985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ07901.1"
                     /translation="MNPPAWIPAHRAFRLTRHIIRPAQTTVILIKTTVRLAQTTVILV
                     QTTIRLAQTAVIQDQTTVRPAQTTIILDQTTVRLVQTTVILDQTTLRLVQTTVILDQT
                     TIRLAQTTVILDQTTLRPAQRAHRPPQLARQRDPPA"
     gene            complement(30601..30864)
                     /locus_tag="PLP58_04990"
     CDS             complement(30601..30864)
                     /locus_tag="PLP58_04990"
                     /inference="COORDINATES: protein motif:HMM:NF024835.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HRJ07902.1"
                     /translation="MDPKFNSMTAAQYEAWVNQRISRTLLRLCVERGLSAYALGRKCG
                     LSDQTILNLEQGKHDRGCLTGNLARICYHLGMTLQEFMNEALG"
     gene            complement(31267..31818)
                     /locus_tag="PLP58_04995"
     CDS             complement(31267..31818)
                     /locus_tag="PLP58_04995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ07903.1"
                     /translation="MDAPERTPKFELAQARELAEALAQHGADYLFLGKAGAILLGYPG
                     TTLDLDLFAPKDAENARRCIAALRDLDFHIDAETEQALLTGKDFVQIKDGPFDVDIIH
                     APDGIESFTAAKSRRIMEDIFPVAHLRDIIASKKASNRKKDLVDMELLEEFARVYAAE
                     KAQPLPSALERAMERHGDNQPPP"
     gene            complement(31805..32011)
                     /locus_tag="PLP58_05000"
     CDS             complement(31805..32011)
                     /locus_tag="PLP58_05000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ07904.1"
                     /translation="MPQTNEETLRLWREQCRRQMQRPLSARLKYGFVRVRNRDHVNRS
                     FATMSEYRAWCEQHQPSYLGYGRA"
     gene            complement(32250..32438)
                     /locus_tag="PLP58_05005"
     CDS             complement(32250..32438)
                     /locus_tag="PLP58_05005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ07905.1"
                     /translation="MNTTAKYHTNLDDLEWLREIRGKITGSFDHTPKRIGDYLREREK
                     TLGDRIFRAQARLVPAKG"
     gene            complement(32435..32851)
                     /locus_tag="PLP58_05010"
     CDS             complement(32435..32851)
                     /locus_tag="PLP58_05010"
                     /inference="COORDINATES: protein motif:HMM:NF021873.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2442 domain-containing protein"
                     /protein_id="HRJ07906.1"
                     /translation="MSTSTTDNQEPAISKLRFEGPLMRVHLSDGRQITVPTTLYPRLH
                     FATQAERRNHRLIAKGRGIHWPDLEEDISLSGLLARKSSAESPQSIMQWLLARKTPAA
                     IGKSSFAEIRISMPERISSKGPLRTRRREIHSHQVS"
     gene            complement(33108..34211)
                     /locus_tag="PLP58_05015"
     CDS             complement(33108..34211)
                     /locus_tag="PLP58_05015"
                     /inference="COORDINATES: protein motif:HMM:NF016488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RelA/SpoT domain-containing protein"
                     /protein_id="HRJ07907.1"
                     /translation="MKYSNKQITKAGETLIKDPQGEDLNAVMDVLSYWRLCHERPLNL
                     AWQELQQVVLRHDKDAIFAKRLKRYASIYKKLKRIDNMTLRNMQDIGGCRAIVGNEKK
                     LLRCVRDLKKLEAFKTEAGRYRAKDYLKHPKDSGYRSFHLVGRFPNERGEMRNIEIQL
                     RTRFQHYWATALEIVDLFTGQALKSNQGDPEWTAFFRGVSDLFAVMDDIFGFQQMPDL
                     EKVETFAQRVHRNPDLLISCAQVQERFKTLRIGKKFEAYANSIKVIDNQIGNSPKSGY
                     ILLVIDTQLKQINSQIFAKDALSEAAVRYATFEKEAATKPDLVVALVSAASVGGIREA
                     YPNFFADSTMFMMLLHMIMEVNPNPRTWARGLP"
     gene            complement(34317..>35151)
                     /locus_tag="PLP58_05020"
     CDS             complement(34317..>35151)
                     /locus_tag="PLP58_05020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018969880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ribonucleoside-diphosphate reductase subunit
                     alpha"
                     /protein_id="HRJ07908.1"
                     /translation="EFMEAVAYYAYEASSDLAAEKGAYSTYKGSKWDRGLLPQDTLEL
                     LEKERGVPVEVPRGGKMDWSALRAKIAKQGMRNSNVLAIAPTATISNIMGSSPCIEPL
                     MSNMLVKSNLSGDFMVLNPYLIRDLKKRGLWTADIQNKLKYMDGEIDAIEEIPVDLKR
                     RYATAFSIDWSWLVDAAARRQKWIDQSQSVNLFFSGKELATLSHMYRGAWRKGLKTTY
                     YLRTVSASNIEKADVQVQKDIRSSVIAGAVGAKREYTAEEVQACSIEARRNGGTCEAC
                     Q"
BASE COUNT         6139 a        10480 c        11650 g         6882 t
ORIGIN      
        1 gctgtcccgg ctggctgctg ccaagagcgg cgcggcttta ccgtccggct tatgtgtgcg
       61 cgtttcatca tgtcagcggc ggtcaggatg acacctggag gctgaacctg ggcggcagaa
      121 tcgtccgcgc ggtgcaggaa ggccgcctgc cagcggccgc gctcggcgcg gggttccgcg
      181 cgtccccttt caaaggcgag gtttttctca cggtggacct ggaggcaggg caggatgaat
      241 gggatcccct ggatcccgcc aaacgcgcag gtgtggaggc ggagggccgc actgtggcgc
      301 tggcgctgtg gcgtgagctg cgcgcggcgc atcccgattt cgcccaatgc ccgccgcccg
      361 tgctgccagc gcaggcgggc attcgcgaga gcgactgcta cgcgggtgat catgtgctga
      421 cgggagatga gctggccgcc tgcgcgcgtt ttgacgacga ggtggcgctg gctggctggc
      481 cgatggagaa aagggagaat gcgcgcgggc cgaagttccg cttttttgag cgggcggagc
      541 ccgcgggcat cccggcgcgc tgcctgcgcc gttccgatgc gccggggttg tttttcgccg
      601 gacgctgcct ctccgccgac catgaggcgc tggcctcggt gcgtgtcatg ggcacctgca
      661 tggccacggg ggaggcggcc gggcgcatgg ccgctgagca cgcatgcctt caacggtgat
      721 gatcctggct gtttgagcgg ccaatgcccg cacatcaggc tcagtcgagc gtgcgtttcg
      781 gcgcgccgat gcggatgccg gggtcgcctt tgccgcgcgc ggcgcctggc acgagggcga
      841 agcgctgcac ctcgtagccg gtgacattga gagtcagatc aagtcccgca agaaactccc
      901 aggaggcggg cacaccagtt tcgtcggcga tcttgcgcgc ctccaggtag gtgtcgaagg
      961 cgttgttcat gacctgaaag agggccacgc cgttcgggtc ggccttgagc gcgcgcagca
     1021 tgcgctggta ggaggagcct tcgttgcgca tctggtcgag agtctctccc gcgccggcct
     1081 tcggtgtgag ctgcatctgc accagggtgg cgccgggccg gatggcgggc acgagggtga
     1141 aggggccgct gaaggtcggg ttcgtggcgg cgcgctcgag catggagcgg atggcgaagg
     1201 cgtcgtaaat gactttgtct ttgctcttgc ccttgatgct gtccaggccg gccaggtcct
     1261 tgatgaagta gctctggatg tcgcgcgtgt tgtttttcac atccacggcc tgggaacccc
     1321 aggtgaaagt gatcctgccc tctccggcaa cggcctggat ggtgggtttg gtcggatcgc
     1381 gcgagggatc gagcgcggtc catttggtca agtcgccggt ggcggcggcg accatggcct
     1441 cggcgaccgc gtccatgttg gaacgcacga cgatggatat gtcccccagg gctgtgagca
     1501 cctggcgggt ggcgggcatg tcggcggctg cgagggcttt gtgcgctttg gccacgtttt
     1561 ccatctggaa ggtgccgaca aagggcgcga tctccgccca tgcctgctgg gcggcggcgg
     1621 gggtgccgta gattttgccg agcatgtcgg cgaaaggctc gatcttggcg tcgcggtaaa
     1681 taagctggtt tctggctttg tcgagtccgt cgagaatggg ttttaaaaat gcgtcctgat
     1741 tgaaatgcac cacgccctgg cgggcgacga ggatgcgcgt ctcgttgggc ttctccgggt
     1801 aggggcgcgg gttgggcagg cggacatagg tgggcggcgg cggctgatag acgggggttt
     1861 catcgagcag ggccttgagc cgctcaattt cggtgatgag cttgtccacc tcgccttttt
     1921 ctttctcccg ggtctttttg gcctcctcca gctgcttgcg gaaggtatcc aagtccatga
     1981 acttcatctg agcggccatc tcggatgtgt ctatggtttt gagttcctcg acgacttttt
     2041 tcaagtcctg ctgagccttt tttttctgct cctcgatttt ttctgggtcg gcgggcggcg
     2101 gtggcagctc tttgatctgc tggaccatct gctggtgctg ctcctcggtg acggggggca
     2161 ggtcggtgag gattttctgg accatcttgg cggtgttgat cgccatcatg acgaagacga
     2221 tcatgagcac gccgacgaca ttggtgacgg catccatcag ggagtcgaga ttcaactcgg
     2281 aggagtcctg ttttttcttg gccatgatct gtgcgggtca ggggttggct gccggctcgg
     2341 gcacgggcgc gccagggacg ggagggtcta tcttgccgcg atacttgtcg aaaagggcga
     2401 gatcgagctg cccgcgcccg gggatgggca gcttgccgac gcggattttg taatcgttgg
     2461 aagcgcggcc tgcgccgttg ttgaaggggc cctggccgtc gtcccggatg aggaagagaa
     2521 tgagggagtt tttgtccttg gccacctcga agaggaagtg gttgtaggcg gcgtcggcca
     2581 cgacgatctc ggcggtggcg ccgaggcggt agtcctcgcc ccaggcgccg aggattttga
     2641 gggctccggc gctgcactcg acgaagtaaa ccctggtgct ctccgccatg ccggagcctg
     2701 agggctgcac aacgacgggc ggcactttct tgtcctccgg cacctggcgc gccttgattt
     2761 cggcgagcag tttttcgatc tcggttttcg actcggtctg ctgctttttg aggccgtcta
     2821 tttccaccag caggctgtcg agctccttct ggagctgctg gctgatgagc aggttctgct
     2881 ctttgatctc cttggaggag tcgagcagct tgcgcagctt gatgtagcgc tgctccttct
     2941 ctttgacctc ctcctggagt ttctccagct cggccagttt ttccttgagg atcaagtcca
     3001 gctccttgcg ctcctcgatc tccttctgca tgcgctggta gtcctgggcc atgcggatct
     3061 cctcggcggt gcgaccctcg gccttgccgg cctgggccac cacgagaacg acgatgatga
     3121 gcacgagcgc cccgatgaga catgcgagga tgctcaggaa ggggaagagc gagacttcgt
     3181 tctcggatga cgcgcgccgc tgtgccatga tgggttgagt gacaaagggt tagtccctgc
     3241 tgaaccagcc tttctttttg acttggtgga tgaggatttg cttgccgcca agctcggtga
     3301 gcacgttgtt gaggccctgg atgccggtgg agagggcgcg cgtgtactcg gtggtggtct
     3361 tgacgctgtc tttgacggcg ttggtgaggt cctccctcat gcggttgagc gtcatggcga
     3421 actcgctgtc cacgcgctcc tggtgggagg cggcgcgttt gtcgaggttg tcggcgtagt
     3481 ccttgatctt ggcggagagg gcgttgaggt ccacgaggaa ctccttctgc gcgttggcca
     3541 cggctttgac gagggtgtcc atgaggccgc cggtgtcgcc gccggccact ccgccgccgt
     3601 cgttgaggcg gggcaggagc acttcgttgc agaaggcgtc aatgttgttg agattgtcat
     3661 cctcggactt ggcgagggcg ctgatgggga agttcaggaa cagggcgagc acgaggccga
     3721 gcagggtggt gtcgaatgcg gtgccgaggc cgctggtgac gttgccgatg gatcccatga
     3781 ttttgtccag atcggaggtg tcgctcaggt caatgctgcc aatggcctgt gacaagccaa
     3841 ggacggttcc gatgaagcct aggatgggga tggcccagag gaagactttg acgagggagt
     3901 agctgccgcc gatgcgggcg ctgtcaatgt tgctctgcgc ctggagcatg gtgttcacct
     3961 cggagttgtt ctggcgcact tcgaaaagct ccaggccttt gcggatgcgg ttgaccatca
     4021 tgctgtcgcg caggcgtccg ggcaggccgt agaggtggtc cacgaagctg ccgacattct
     4081 ggccgttgat ctcctggccg aagtcggtgg gcagcacgtc gaggtacatg gcgtcctttt
     4141 gatggcgcag ctttttgcct ttgagccaga ggatggcgat ggcgaagaag aagaagaagg
     4201 tctccagcat gttcacccag ctgcgctcgt aaaagagatc gtgaatgtag tcgagcgtgc
     4261 tggcggctgg gtcgccatat ttgccgacgc cgaagggata aaggatgccg taccatgtga
     4321 gggtgagcac gagcccgatg ccaagggcgg cgaaaatgtt ggcgttggcc ggatcggcct
     4381 cctcccagcc gccgcgggcg ctctgctgct ggtaagactc ctggtactcc tggtgcgacc
     4441 ctggctgctc gccgggcgcg ctggcatcct ggggtggagg gctggccgtg gcgggcacgc
     4501 cgtcgggcgg cggcggcagg cctgagggtg ggggaggcgc gccgatggtg gcgggaatgc
     4561 tgagcttggt gttgcaaccg gggcagcgga cgattttgcc ggccatttca ggctcggcct
     4621 tcagttgcat gtcacacgtc gggcacttga atttcacaat cggttctcct tgggtttggg
     4681 ttggtgtggg tgggtttgaa aatcggctgt tgacgattca agcggccaga ggccggaaga
     4741 acaagctctt ttgtgccatg aagacgcgct ttccgcgcat ttcatggggt ctggcgctga
     4801 ttcaaggatg cggcgtccgg tgaaaacgtc aaaaggtgag ccgggcaccg gcggttttga
     4861 gttcctcggc gtgctttttc gcctcctcgc cgagcgggtt gccgccgagg tggatctggc
     4921 aatacggcgc ccactcgcgg gctccgtcgc tgtccttttt ccacatgcgg tgcagcgggg
     4981 cgatgtcggt gatctggttg ttttcgagga agaggaactg gaggtcggtc aaaggctcga
     5041 gcggggtgag accggctacc ttgttgcctt tcagcgagag catggagagc catttcatgg
     5101 agccgatgcc ttccaggttg ctgactttgt tgccttcgag gtaaagcgtc cagaccttgg
     5161 gcagcttaaa gagcggggtg gcatcctcga tctggttgcc cgccagatag agggaggtga
     5221 gcgactggat gccgccgagc ggagcgacat tgacgacctt gttgccggtc agctcgacgt
     5281 attgcagagc cttgcactcg gaaagggggg agatgtccgc gatctggttg ccggcgaggt
     5341 cgaggaactg caaccgcgcc aagcccttga gcggggcgag gctggtgatc ttgtttcccg
     5401 gcaaagtcag cgaggccagg gcggcgcatt tttccaggcc ggagagatcg gtgatgccca
     5461 tgtttttgcc ttcgatgatg gcgacattgg cgacatcctc ggcggtgagc gggtctttgc
     5521 tctcgcgttt ggcgaagacc tggcggcgca cggcgtcttc gagggccttg tccttgaaga
     5581 cgggcaccgg cttgggcggc ggcggtggct cggctttctt tggctcctcc ttcttggccc
     5641 cggcggtttt ggcgggttca ggtttcggcg cgggtgctgg cgctggagcc ggtgcgggct
     5701 tgggttcctc ctttttggtt tcagcggttt tggcaggatc gggcttggga ggggtggcgg
     5761 gagccggtgc tggcttgggt tcgtcttttt tggcttcggc gggcttggca ggctcggcct
     5821 ttggcgcggg aacaggcgcg ggcttgggcg tctccgcctt gggggctgcg gctggcttgg
     5881 ggtcttgcgc agagacgttc agggtgatga gcaagaggat caggagggct ggggtcttca
     5941 tgaacggcgt gtttgaaatc aggcggttat tacggcagcg cggggttgca tgtttgcgca
     6001 aaatcggctc aatcccggct catggtcaat gtcatcgtca ttctcgaaat agacccggcg
     6061 cgcgtggacg aatttctgga ggtcatcact ttcaacgccg ccgagtcggt gaaggagccg
     6121 ggctgcctgc gcttcgaggt gagccgccag tttgaccagc cgaacctctt tgccctcagt
     6181 gaaagctaca cggacaaggc ggccatggac gcccactatc agacgccgca cttcgcgctg
     6241 tggaaggaga agtccgccac cggtttcgtg ctcaaccgct ggtcggtgaa ggggccagtg
     6301 ctcgaataat ccctcccgtg gaagacgcct acggccaccg catctcctac ctgcgcgtct
     6361 ctgtgacgga ccgctgcaac gagcgctgcc gctactgcat gccggaggag gagcaggcat
     6421 ggtttccgaa ggaggaggtg ctcggttacg atgaaatgct gcgcgtgctg cgtgtcggcg
     6481 cgcgcctggg catccgcaaa gtccgcgtga ccggcggcga gccgctgacc cggccgggtg
     6541 tcgccgactt ttgcgccagg gtctcggcca ttccgggcat tgagcacctc ggcatctcca
     6601 ccaacggcac gttgctggcg aaaaccgagg gcggtctcac cctggcggag catctcgccc
     6661 gtgccggcgt ccgtgccgcc aacatttccc tcgactcgct cgaccgcgcc gcctaccagc
     6721 gcgccacgag ccgcgacctg cttccgcgcg tgctcgaggg cgtggaagca gcgctcgccg
     6781 caggcatccc ctccctgcgc ctgaactgcg tgctgatgaa aaaccagacc gagcgcgaaa
     6841 tccccgcgct catggagtat gcgcgttcga agggcgccct gctgcgcttc atcgaactca
     6901 tgcccgtgag caccagcgat gtgctgcgtg atgaaaactt cctttccgcc ggccatgccc
     6961 tccgtctcgt ggagcgggag cacgggccgc tggcgccgct gccggacttc aagaccaatg
     7021 gccccgccac atattaccag gtccgtgcca caggccagaa aatcggcttc atcggcgcga
     7081 tgacgaacct gcacttctgt gaaagctgcg acaaactgcg gctgaccagc gacggcaaac
     7141 tgcgcccctg cctgggaagc tggcttgaat tcgacctgcg ctccgtgctg cgcgcgggct
     7201 gcacggacga ggagttggag ggcttcattc taggcgtggt ggcgcgcaag cccaaggagc
     7261 acgacttccg caacaactac ctgcccaacc gcaaaatgat cgccatcggc gggtgacgga
     7321 cgagccgccc ggactcacag ctctgcggca gcactctcct gtccgggctt gaatgcagcg
     7381 cggaccgaat cccgaagcag aacgataaag gtgaacacgc cgagagcggt gccgaaggga
     7441 aattgcaggc agttcagccc cgcgatgacg aggcagaaga catggtgctt tttcctccgc
     7501 aaagaaaaac ccgccgccag gtttgcgaag cccaccagga ggatgagcag gcctatgccg
     7561 aggtagaacc attgcatgac atcccagatg acttccggcg gcggaccgtc cttggcattt
     7621 ttccaaattt ccgggctgcc aaagaccatg cgcatgatgc cgtaatggag gcccaggaaa
     7681 cccatgccca gaaggctgag gccggccagc acccagtgaa agatggccag caggcgcagg
     7741 tgctcatcat ccttcacacg cgggtcgggc agaggcgggg gcggggcgtt catgactggc
     7801 gcagcatacg gcccggatcg taaatgccaa gcgccaaggc ccgcgggaat actgaagctc
     7861 aaaaccgcca gacaaggccg gtgtgaagcg tgaagtccgg actgacgccg gtgaggcctc
     7921 cgccggcgcc gaggcgcaca atttgcgcac agtggctggc agcagagcaa gagtcagaat
     7981 gggataagct tcaatgtgcg cggtgagggt ggttcatgcc tggggacgat ttttcttccg
     8041 gggcatggtg gcgaagaaga gggcgatgcc gagcagggcg acgacgcagg tggcggtcgt
     8101 cagcgtggtg agcacgaggt cgcgcatgtt tttgtcgggg atgaagccga gcttgtggaa
     8161 gttggtgaaa atgcgggcct ccaactgcct gccgtcatcc gtgtgccgcg tcacgctgcc
     8221 ggtggtggtg gagacataga cgcgggtgtg gcgctcgtcg tcgagatcaa agcggtacac
     8281 cggcaggatg cggaagatgt tcaggtactc gttgtcgaag gcggtgagat gggcggtctt
     8341 gcgcacagcc gcgccggcaa ggaaacgggt ggcgatctcg gcggcgtagg cctcgtcctg
     8401 gccgggatcc acacgcccgt ccacggcgct gacataatgc gcggcgcctc cgttttggga
     8461 ataaacctgg taccaggggc tgccgccgat gccgcgcacg ttcacggcgg tgacggcccg
     8521 cgcttcgccg ggcagctttt ccagcgcctc ggccacaccc agccggatga gcgaggcgtc
     8581 cagtgtgcct gtgggcagcg cgggcggtgg cggcggctgg ctgcgcgtca tgacgttgtg
     8641 aatgacgccg ctgcccgctg ccatgaggat gctggcgctg aagaggaggc ccagccagcg
     8701 gtgggctttg cggagggttt gtttgctcat gagattttgc cgccggggcg gggcgctccc
     8761 gccccggcat gggttcacca tttccattcg atgccgccat acagcgcccg cccatctccg
     8821 ggaaagaaga ccgcgctggc gggtgtggcg gcggtgacca cacctgtggt ggccgcgtaa
     8881 gttttgtcgg tcaggttccg cgcctcgagg aaaaaggaga ggcccttggt ggcgcggtag
     8941 ccgactttga agcccagcaa ggcgtagccg tcggccttca gggtgttggc gaggtccacg
     9001 aagtaatcct gcggcgccca ctccacgttc ggtcccatgt agaacccgca cgggtgctcg
     9061 tagagcagct cggcgcggta aaagtggcgc gggatgccgg gcagttcgtt gttcgagaaa
     9121 tccttgtcgc catcaaagtg gaagtcattg aacagataaa actggcggaa aaagaggcgg
     9181 tcgcccgggc gctcgtcagt gccggcctgc ttgccagtcg cgggtgaggc atcctgccag
     9241 gaggtgagga cgccgcgcac cagatcgaag tccagcgagg cctcgatgcc gtgccggatg
     9301 gtgcgtcccg cattgatcgt ctgggtggcg ctcacacccg gcacgccgag ggcgaagagc
     9361 tcgttgtcca gccaggtgta ataccaggcc acgtcccagc gcgcacgccc ggcgctgccg
     9421 cgtgtgccga actcgatgct tgtgccgcgc tgggcgtcca gctcgatgag tcccggcgtg
     9481 ccgccgagcg cggtcagttc gccgaaggag ggaggctcga aactgcggct ggcattgaag
     9541 aagatctgcg ttcctggcgc ggcatcccag agcaggccga gctttggact gaagccccag
     9601 tattcctggc ggtcgctgtt gtccgcgccg aagacgcgct gctcgtcgaa gtcgcggttc
     9661 gcgtagctgc cctgcgcgcc gagcaccagc gccaggctgt cggcgaggtg gagggtgtcc
     9721 tggaggtaga ggtccacatt ggtggactgc tgctggtttt cggcggtgcg cggaccgcgc
     9781 cggcccagca agttggcgaa gcggttgtcc tgcgtgaggc cgtacacata gcccaggccg
     9841 agggtgaaat gattccggtg tccggccagg tccgccagcg agtcataacg caggtccagg
     9901 ccgaggtcgt tgctgagctg gtcaatcacg cccggcccga gcagcaagcc ggggccgaaa
     9961 gagatgagcg gatgatccag atccttccac gaccagaacg tgctcaggga aaggctgtgg
    10021 acatcgtcgg accatgtggt gcggttggcg aggcggaaga gttcaaagtc acgcttttgg
    10081 ttgaacctca cattggcggc attggcctgg tccggcgcgc gctcgagctg gcgctgggtc
    10141 agacttcccg gcagttgcga gtccgtgagc acataggtga ggtaaaagcg cgtctccagg
    10201 ttctccccca gcttctggcc gatgttggca aagaagcgct ggctgtcctg gcggctgtgg
    10261 tcgcggtagc cgtccgtctc attgtaggtg aaactggcgt agtagtccgt ggcgccggag
    10321 acgccgcccg agctgatctg gccgcggtag ctgccgaagc tgcccatttc aaagcgagtc
    10381 tgaaatggcg acgcctcatg gccggtcatc gagacgaagt tgatggcgcc acccagcgtg
    10441 gtggagccga actgcagcgc gttggcgccg cgatagacct caatgtagcg cgcggataga
    10501 ggctcgatgg cctgcatgtc aaagccgccg tcagcgaggt tcagcggcga gccgtcctgc
    10561 atgagcttga tgccgcgccc gtggaaggtg cgctggatgc ccgagccgcg gatggacaca
    10621 cggctctcct ccgcgccgaa gcgcggctgg atgaagacgc cgggggcgaa gtcgagcgcg
    10681 tccttgagcg tggcggcgcg cccgcgcttg tactcctccg catccaccac ggcaacgccg
    10741 ccgggcgtgg tggcggcgag ttcctggcgg cgggtttcga gtttagggac ggtgcgtgac
    10801 gggtcggggg cgtccgcggt gacgagcacc tgcggcaggg tttcagaagg cgcggcggtc
    10861 tgcgccggca gttgggaaaa ggcaaggagg cccgtgaggg gcagaaaaga agggagagat
    10921 ttcataaagc tgtcaccggc aaaagggccg gagtccggtt ggggagtgag cgcggcttgg
    10981 gcaatgccgc agaggaggtc catgcgcgcg ccggcccagg gcgcgctgtc aaatcaggcg
    11041 cggcaagagc cgcttcgcgg tggacggaac cgtttttcct cccagccggt cgtccggcat
    11101 cccctccatt cgggatgctc aaaggtgacg cccgcaggtg tgacgaggcg gggcgcgccg
    11161 gcaagcacgg cgtccagctt caagaccttt tgcagcatag tctcgcgtgt ttccctgccg
    11221 ctcttctcca ccgctttgca gagcggacaa gaatgctcgc catcaaaggt ggcgctcaaa
    11281 ccttcgacga gggagccttt ttccaacgag taagtcaccg ccatgccgag ccatgccacg
    11341 gactgcgcca gcatccacgg gatgccgagg gcgatcatga gcgcgcctgt gacagcgagc
    11401 ctggcgatgg aagggcgggt aaacatgcgg tggcaagcgg gcggaggggc ggccctccgc
    11461 ccgcgtggat gaaacggaat cagcctttgt gcgggtcgtg gcaggctttg cagttcacgg
    11521 ccttcttgta gtcactgacg tccttcgggt ctttttggag ttttttgacg gcggcgataa
    11581 gggcggcgca tttttcctcc caactgctgg gctggccttt ttcaggcttt tccttggcca
    11641 tggcctcgta ggacttgagc aactcggcga tctggtcgga agtgccctcg ccattgctga
    11701 cttgtttgca cacagggtcc tcgccttgcg gggccttgtg gtattttttc atgatgtcgc
    11761 tgatgacgcc gtcggcgcgg gcgctggcag cgccggcgag gaagaccatc gcgaatgtgg
    11821 tgatgaggat gcgttgtttc atgggtggta tgtgtgtggt ttaaaggcgg cggcgggtgc
    11881 tggccagcgg ccgtcattcc cggttgcggc cttgacgggc ctggcaggga aagagtagcc
    11941 gcccctgtct ggaaaccggc cagacagggg cgtcccggcg gatcatggaa ctagcggaaa
    12001 ccatgctctg ttctccggga aaggggtgga aaactcaatg agagggcgcg tgatgcgcgg
    12061 tgaaactcgt catgcggggg catggcagca acccgcgtgg acacggcaca agtctggaat
    12121 ccctgatcac gctttcaagc gctcggccct gacggcatga cgggcatgaa gccgccgccc
    12181 tgccgcggga agacccagat cagatgatcg ccgcgagccg gggaggcggc gcggtcggct
    12241 ggacttgacg cgcttccgcc gtgctggaga tcgtgctcca agccgggggt ggcgctgttg
    12301 tgaacacaaa gacgggcacc ggcacgatgg ccagggtgat cttcaagtcc ttgccgggtt
    12361 tgacaggcgc ggaaccgttt ttggcggggc tttccttgac gccctcgctc actgcggtgc
    12421 ataggggaca tggatgctcg ccgtcaaaag tgtcgctgat gccctggacg acggagccct
    12481 tctccacgga ataggcaacc accattccca cccatgccgc cgactgcaaa accgcccagt
    12541 gcagcccgat ggagatcatc aaggccgcga cgagcagggt gcgcgcgacg gttgtcaaca
    12601 tgcgtcaaat aaagcggctg cggcggctgt cttgcaacct catttttcat cccgcgccaa
    12661 aaaacacgct ttacgtttcg tttttgatcc tggtataacc acgtccatga aaaacaaaat
    12721 cctctccgcc gctccgtccc gccgccaatt catcctcaac ggagcccagg ctctcgccgc
    12781 cggctgggct ggcagcagcc tcgccgaaac cgcctccatg ccgcgctccg aaacactggt
    12841 tcagcagctt tacggcagcc tcaaagagga gcagcgcaag gcgctctgct tcgcctatga
    12901 cgacccgcgc cgcctgaaag tggacaacaa ctggcacatc gttccgcagc gcctgcgcga
    12961 caccctcgga gcggaccagc aggacttggt gcggcagatt ttcgacggtc tgcacagcga
    13021 ggaatacaag aaggaagttt ggcgccagtt tgaccatgac aacaagggtg atggcggctt
    13081 cgccagctcg tccatcgcgc tttttggcga gccggggcag ggcaagttcc agttcgttct
    13141 caccggccgc cattgcacgc gccgctgcga tggggacagc gaggccggag tggcctttgg
    13201 cggaccgatt ttttacggcc atgcagcgaa gggattttat gagaagcccg gccacgaagg
    13261 caacgcttac tggttccagg cgaaacgcgc caacgaggtc ttccaggctt tggatggcag
    13321 gcaaagggag ctggcattgc tgggtgacgc gcgagacgag gcggggaaca agaccattaa
    13381 acttaccggc aagacgaagg ggctggaagg catcccgatg acggaactca gcgcggatca
    13441 aaaaggcctc gtgcgcctcg tgctggcgga tttgctcaag cccttccgca aggaggacgc
    13501 ggcggagaca ttgaagctca ttgaagcggc gggtttcgac cacctgcaca tggccttcta
    13561 caagaaccat gacgtgggcg aggacggggt ctgggatgtc tggcagatcg agggcccctc
    13621 catggtgtgg tatttccgcg gtgatcccca tgtgcattgc tgggctcaca tccgcgagag
    13681 cgcgtgagac gcgcgcgggg ctgaaagttc gcgttgccag ggcggggccg gtggttatgg
    13741 tcgcgcccga atgatccccg ccaaggccat cctcgcgatt cgaattagcc acgggctcca
    13801 ctgcgcctga cgcagtcctg gagtccgtga cggcctttac gacgccttga cggcgtgatt
    13861 gcgtggcatt tccgcgtcca ccgggacata ccggcttctc cttttcaacc cttcttcaaa
    13921 actccatttt tcatcataca tgtcagtcac catctacgac accaccctgc gcgacggcac
    13981 ccagggcaca ggcatctcct tcagcacgct cgacaaaatc cgcgtcgctg aaaaactcga
    14041 cgacttcggc gtccactaca tcgagggcgg ctggcccggc tcgaacccca aagacgcggc
    14101 ttttttccag gaagccgcca aaagaacctg gaaaaacgcg cggatcacag cctttggcat
    14161 gacccggcgc ggcaagatga aggtggagga tgatccccag gtgcggatgc tgctcgacgc
    14221 gcggactccg gcagtcaccg ttgtcggcaa gacctggtcc ctgcatgtca ccgaggtttt
    14281 ccaggtcagt ctggaggaaa acctggcgat gatcgcggac acggtggcct atttgaaaaa
    14341 acaggggcgc gaggtgctct atgacgcgga gcatttcttc gacagctatc gcgaggatcc
    14401 tgaatactcg ctcaaaacga ttcaggcggc gcaggacgcc ggggccgatc tagttgtgct
    14461 atgcgagacg aatggaggcg cgctgcctga atttgtcgag gaggccaccc gcaaagtcat
    14521 cgcgcacctt gggcatcctg tcggcatcca cacgcacaat gatggcggcg ttggcgtggc
    14581 caacgcgctg gctgcagtgc gcgcgggtgc ctgccaggtg cagggcacga tcaatggcta
    14641 tggagagcgt gtggggaact gcaatctgac caccgtcatg cccgcgctcc aaatcaaaat
    14701 gggcgtgccg ctcggcatgg acctgacccg cctgcgcgag ctttcctttt ttgtggacga
    14761 gctcgcgaac gtgccgcacg acattcgcgc tccttatgtg ggcctggccg cctttaccca
    14821 caaaggcggg ctgcatgtgc atgcggttca gaaactggcg cgcacctacg agcatgtgga
    14881 ccccgccctg gtcggcaacg agcgcgtcat caccatctcc gacatgtcag gccagtcgaa
    14941 cgtgctggtc aaagcggagg cgatgggtct tccgctggcg aaaggttcca aggaggtgca
    15001 gaaaattctc accgaagtga agcggctgga gagcgaaggc tacgagttcg aggcggcgga
    15061 ggccagcttc gaactgctga tccgccggat gctgggccag gagacgcatt gcttcgaact
    15121 attggagttc cacaccacgc accgcttccg ctccgggatg accgccgaga ccaacgaagc
    15181 gacggtgaag ctctttgttc atggcaaggc ggagtacacc gtggacgagg gcgacggccc
    15241 tgtgaacgcg ctggacaagg cgctgcgcaa agcgctgctg ccgcactttc cgcagatcgc
    15301 ctccatgcgg ctggaggact acaaggtgcg catcattgac agcggcagcg gcacggcggc
    15361 gaagacgcgc gtgctcatcg tcagcacgga cggcgcccgc acctggggca ccgtgggcgt
    15421 gtccaccaac atcatagacg cgagctggca ggcgctggtg gacagcatgg aatacttcct
    15481 tctgcaagga aggtaacccg gcttcacacg caaaaaagaa ggccgctttt cagcggcctt
    15541 cctcatgagg cgggtggcca aatcggatgg ttcaaggcac gaacaactgg atgtcgccag
    15601 actggatttc gggggtcggg tccgagggca gcgcgggcag ttggaagttg ccgcgcccta
    15661 tgaagtccgc cggattcgtg ctgagcttgc ggcgcaggac gccgctgaaa ttgaccgtgc
    15721 gggttttttc attcttgagg gtgaacttgc ccgtgaagcc gccagtggcc gtgttgatgg
    15781 tgatctgcca caccgcagga ttgccggcgg gtgtctggac ggtgactttg ccattggcgg
    15841 ccatcgtcac ttccgttggc aggagccccg catccacgcc ggcgaagccg ctgtggccga
    15901 tggaaatgac cgtgctgtcc gtgatgccaa gctcattaag gagggcgatt tcaggcagcg
    15961 tcttggtggc cttggccggt ttgcgccagg ggtccagggt gatgccgcag tccatgaggt
    16021 ctatgccgcc gcggtaggat ttgtccttgg ggccggccgc tttggcccat gcgaggctgt
    16081 cacccatgag catggtgggg atgtggccgc gccccgggag gtcggggtgt tcgaggaggt
    16141 cgagccatcc agcgaggtag ctgtcgagcc ttttgtaggg gttcacaaac agccggtagc
    16201 ccccatccac tccgggcatg agcgtggcgg tcgtcttgat tccatccgcg agggtgaggg
    16261 tgagcttcat gaccgccttg gcatcaacgc tgccagtggc atggccggag ccgagcggga
    16321 tggggttgac cgcgccaaag gccggcaagg gcggtgccag gatcagggtc agcaggccgg
    16381 cgttggtgac gatctgccct ttggccggca cgaagacgcg cgcgccgtca tccgcctcgg
    16441 caaacagatc gccgtccaca tccaggatgg cctcgaatcc tccgccgatg tcgaggttga
    16501 tcgtcatttc ataggctatc gcgaccttgt tgacgagttt gttgagtgtc caggttcccg
    16561 tcgcggtctc tgtgagcgga tcaacggtga gcgtgccctt gattggcagg gcggtcgtgt
    16621 ccgtcggtgt ccagagtttg cctgtgaacg ccattttgtt gtccttgatg gtcttggcca
    16681 ctgtcagttc aaccttggcg atggcgcgca tggtgtcggg actgaccagc aggttttcgt
    16741 agcgcccttc gaagctgtcc acgacgagag ttcccggcac gaaaacaaac cggtagttca
    16801 cagccagccc gcctttgaag gtgatggggt atctgccgcc cggactcagg tctttggcgg
    16861 tggtggagat gaccggcacc gtcgccgtgg gcttggcggg gggcgcgggt ggcggcggga
    16921 aaacggagtt ttcgtcatcc gcgccgagga agccgctgta gttgaaggtc agggccggat
    16981 tgggctggcc gatgagcttg cgctggttgt cggcggtgat ggtgagcggc gccttgttga
    17041 tggtgagctt gccggttttt ttcagagtgc cggaggtggc gaggacattg tagctgcctg
    17101 cgttcaccgg caggttctgg ctgccattgt aggtgatgac gaggccggtc aggggcacac
    17161 cagtgacacc gacactgaga ggaccgcccg tgtaggtctg cacaagattg atgagattga
    17221 atttcgtaga gaccggaacg acgtgaaagt ggcgggccac aggcggtgcc ggatcaaatg
    17281 tggagtcgcc agcctgggtg gcgcgcacct gcaccacacc cgcagcccct gtgagggtga
    17341 cggtggagcc ggccagattg gcgggaccgg agaggatttg aaaggacacc ggcaggccgg
    17401 agctggccgt cgccatgaga tcaaagggcg cgtcacccac ctggcgctgc gcgggcgcgt
    17461 cgaagctgat ggtctgggcg agggtgccgc cggtctggat gttgatggtg acagtggcgg
    17521 tgttggaatc atccgtgccg tcgttggcct tgaaggtgaa ggaatcctga ccgctctcac
    17581 cgccgtccgc agtgtaggtg aaggtaccat tggagttgaa agtgaggtct ccttttatcg
    17641 gatcagccac cttgctgaaa gtaagcgagt ccaggtccgg gtccgtggcg gtgacactgc
    17701 tgttaaaagg cacgcccgcc aggacgttga ttgttttgtt ttcagccaca ggagcgttat
    17761 tcccgctgga gccggtggcc tgcatgcgca ggatggtcag agggccgctg ccaaagccgt
    17821 agtcgaaggt tccaggcccg gcggctccgg catagagggt ggcgatcgtc cccggcctga
    17881 ctggcacctc aatggggcca aggggcggca gatcctccgg gtcagtgggg aagttttggc
    17941 tgagtttgac caggaagtat tcggtggcgg aggtgcggct ggctcctttg tatagccagg
    18001 catagatggc attgccgccg aagttgttcg ggtctgacga gacgcgggtg tcaaagctca
    18061 cggctttttc caaagcgcca tccggcccgg tgctcgtgag gtccagggca aacggggagc
    18121 caaagacacg aaagtcggca ttgagcgcgg cgatgttccc ggaggccaca tgcgtgttca
    18181 cctgctcgtc cgtgagggtg gccatgcggc caatgacgcc gccgccagtg ccgggggcga
    18241 tgcgggttga ggcattgtct gagatcgcgt gaaaaccgtt ggcagcagca ttgtagttgg
    18301 agacggtcag tgtggccgcc tgggtgacat gggccgcgca tagcgcggcg caggccgcga
    18361 aatgaggaaa tcgtttcatg aatgagcagg gggtgggcta gattcaaaaa actcagaagc
    18421 tgccctggtt gagcgtgatc atcacgggag cgccgcggcg caggatgatg taggctccgt
    18481 ctggcagggg cacggacgat tgatcggtgc tgccggcgcc gactttgcgc catccagcgc
    18541 cgagcgggcc gcctgtggag ttgaaatatt gatcgtagcc ggagccgttc cagagcagaa
    18601 cgagatcggc cagactggat gccgtgccgc cagccatgcc ttcggcgagt cctgagttgc
    18661 ccagtgtcct gccttccacg gaggcgcctt ctccggcagc gtttacgggg cagaggttgg
    18721 ccacatagtt gttgccggtt tgcaggctga ccttggtgac acccggtttg acctggccga
    18781 tgaccactac ggacttggcg gcacgggcga tgatggccgc tccctcgctg acgaaaacgg
    18841 gcacgtcagc ctggtccgtg gttcccccat ccacctgccg ccagcccaca ccctgcgcgc
    18901 cgccagtgga atagaaatac ttgtcaaagc ccgtgccgtt cggaagcagg atcaaatccg
    18961 cctggccggg tgtctcgcct ccggcgagtc cggcgctgtt gtccgccccg aaaacatctg
    19021 ccagcgtcca aagacgccaa actttcaaac tcgcgccgtc ggcgactccc gctggcactt
    19081 cctcctgcag ggtgagctgg tttccggtgg acagtgtcgc ggtgactatc gtggtgaatc
    19141 cctggcccgc gccgctggag acgatctcca ccgcatgggt ggcgagcggt ccggggttga
    19201 actggttgtc cgccaacgca aggttggggc cgccttggag gaacacgatt ttgcggtctg
    19261 ttggcgagat gttgaagctc gactgcatct ccatcgtggg cagcagggcg aagccgatga
    19321 agttcgttcc ctcctgcaaa tgcagggtca ggaaccccat gatgggagat gtgacttggg
    19381 cttggagcag accggtcgcg gcgagcacgc cgaagattgc catgttaagg agctttttca
    19441 tatgagtcaa tgagggcggt tttgggcgtt tattggactt tccggggata gtgcgggttt
    19501 ttcgaggaca aaacaagatt aaagaacggg catgtcgcgc ccagcctgaa gcaggcgggc
    19561 tgaagtttgg atgaagaaga cacgaagttt ctctgatgag acggggattt catcatgggg
    19621 tcagcgggct agaccgggcc gcctcgtgac aacaagacgg ccaatctggc tctccagtgg
    19681 tactaacccc aaaatggggg ccgcgttaca cactccgcat tcaattctgt cgggccgccc
    19741 cgcttcggac ggttttatca tggcagcttc tggcggccag cctgcggaga agtccgtttc
    19801 atggtaaaga cgccgcatcg ccaccttgcc cgccgggaat tcccgttgaa tgggtgaggg
    19861 cgtgattgcc ccctggatca tcctgtcttt tctgctgctc agcgcgctcg cgggctcgct
    19921 gcaaaaactt tgcccgaaag ccgcgccctg gctgctggct gcgggtccgg gtttggcagc
    19981 gctctggctg ctggcgcaga tgccgggcgt ctcagcgggg gcggcgccgg ccttcagcgc
    20041 gccgtgggtg cctgagctgg ggctggagct ggccttccgc ctcgacggcc tgggcctgct
    20101 gctggggctg ctggtgtgcg gcatcggttt tttcatcgtg ctctacggct cggcctactt
    20161 cgagaaggag ccggtggcgc gcgggcgttt tttcacgctc atcctcgtct tcatggcggc
    20221 gatgttcggc ctggcggtgg cggatgattt gctgctgttt ttcctattct gggagctgac
    20281 gagcatcacg tcctacctgc tcatcggctt caagcacaag gaggagtctg cgcgtgaggc
    20341 ggcgcagcag gcgctcatca tcaccgctgg cggcggcctg gcgctgctgg ccggcctgat
    20401 cctgctggga gatgcggcgg gcacgctgaa aatctccgag ctggcggcgc gggcggagtt
    20461 ggtcaaggcg cacgcgcttt acccctggat cctgggcctg gtcttcctcg gcgcggcgac
    20521 gaagagcgcg cagtggccct tccacttctg gctgccgggg gccatggccg cgccgacgcc
    20581 ggtgagcgcc tacctgcact cggcgacgat ggtgaaggcg ggcgtcttcc tgctggcgcg
    20641 cctgttcccc gtgctcggcg gcactgagct gtggcagaac ctgcttggcc tggccggcgc
    20701 ggtgacgatg gtgacagcct cggtgctggc gctgggccag cgggatctga agcggctgct
    20761 ggcctacacg acggtgagcg cgctgggcac tctggtgatg ctgcttggca tcggcgacgc
    20821 gctctcgctc caggcggcgg tggtctttct gcttgtgcat gccttttaca aaggcgcgct
    20881 cttcatggtg gcgggcgcgg tggatcatgc gacaggcacg cgcgacatca acctgctcca
    20941 gggcctgcgc cacctcatgc cctggacggc ggccggcgcg gtgctggcag ccttttccat
    21001 gtccggcatt ccgcccttca tcggcttcgt ggccaaggag ctgctttacg aggccaaggt
    21061 gagcgtgccg gacctgcgca cgcccctgct catcgcggcg gtgttcgcga acatggccac
    21121 ggtgaaggtg gctttcagcg tgggcctctc gcctttccac ggagcgccgc cggaggagct
    21181 ggagcacgcg cacgatgccg gctggcgcat gaagctgggc tttctcaccc tcggcgtgct
    21241 cagcctcgtc tgcggcctgg caccgcagat ggtcaatgac ctgctgctgg ccccggcgct
    21301 gcgcgcgctg gacccggagg cggcggtgct gaagctgaag ctctggcacg gcttcacgcc
    21361 ggtgctggcg ctcagtgtgg tgacggtggc gggcggcatc gccatgcatc tggtccggcg
    21421 gccttttcag aaaatccgcg cccgcttcgc cggtgtggcg gagtatggcc ccggcgcgct
    21481 gtatcaatgg aagtggagcc tgctgctggc ggtggcggac ggcatccagc gctggattca
    21541 aaatggccgc ctgcgccatt acctgcgcgt gattttcatc ctgaccaccg gtctgctgct
    21601 cctgcgcgtg ctgccggtgc tgcccgagct ggcgcgcggc acggagctgg cgccgcgcgg
    21661 ctttgagaca accgtggtgg tgacgatggc cctggccggg ctggtggcgg cgctgacacg
    21721 ctcccgcatc acggcactgc tcggcctggg ctgggtgggg ctgggcgtga cttatatctt
    21781 cgtggacttc ggcgcgccgg acctggcgct cacgctgatc ctcgtggagg cgctgacggt
    21841 ggtgcttttc gccgtggtca tcacccgtct gccgcccatc cgcccgcgcg atcctctgag
    21901 cggacgcctg cgggacgctg gcatcgccct gctggccggc gccgccgccg gcatggtagt
    21961 atggaaggcg gcgcatgtgc ggctgcatga gagcatctcc ccggaattca tggcgcgcag
    22021 cctcagcgag gccaacgggc gcaacgtggt gaatgtgatc ctggtggact tccgcgcgct
    22081 cgacacgctg ggagagatca tggtgctcgg cgtggccgcg ctcggcgtct atgcgctggt
    22141 gcgcatgagg tccgcgcggg aggacgaact tggcgcggac aaacaggaaa aggaggacgt
    22201 gtcatgacca gccgcatcct gcgcaccgcc gcgcgcctca tctcccccat gctggtggcg
    22261 ctggccgtca ttctcatgct gcgcggacac aacgagcccg gcggcggctt catcggcggc
    22321 ctggtgctgg ccatggcgct gctgctgccc gcttttgtcg agcaggaaac gccgcgtctg
    22381 aacctgtggg tggacccgca gcaattgatc ggcctgggct ggctgctggc gctggcctcc
    22441 gcgctgctgc cgctgctggt gccggggcac ggcttttttc aggcgctctg gggcggctcc
    22501 atctggctgc cggtggtggg gaaagtgctc ttcggcacgc cgctgctctt tgacaccggc
    22561 gtcttcctca ccgtggcggg cgtgactttg aaaatcgcct cacttttcat gcaggctgcg
    22621 ggtaatgagg gccggggcgg gctctgaccg gcctaaaatg agacattagc ccgccaaggc
    22681 tcgacaaccg gacaggaaag gccgattcat cccgcgcaaa catccccacc atgtcagacc
    22741 ccgtttacgt catcggccac cgcaaccccg acaccgacgc catctgcgcc gccatcggct
    22801 acgccgccta cctgcgccag gtgcggcagg atgaggtggt ggccgcctgc tgcggcgaga
    22861 tcaatgcccg caccaactgg gtgctgcgcc aggccggcgt ggaggcgccg agactgctgc
    22921 tcgatgtgcg ccccaccgcc gccatcgtct gccgcaagga ggtgctcacc gccggaccgc
    22981 gcgagacctt cctctctgtg tatcgcaaaa tgctcgagca cagtttccgc tccatccccg
    23041 tcgtggatga aaacggccgc ctgcttggca tgcccaccat tcaggaaatg gcgcagcttt
    23101 ttctcccctc cgatgccgcg cacgccgccg ccaaccgcga ggtgcgcacc agcctggcaa
    23161 acatggccgc tgctctcggc gggcgcctca tcggcggcgt ggagtccgcc gacgcggtgg
    23221 aggacctgct gctcgtcgtc gccgcctcca gcgtcgagac ctcgcgcgac cgcgcccgcc
    23281 agttcccgcc gcgccaggtc gtgcttgtca ccggagacag gccggagata cacgcgctgg
    23341 ccatcgagct cggcgttcgc tgcctggtcg tcaccggcgg cttcatgccg tgggacagct
    23401 tgctgcaaca ggccgccacg aaaggcgtct gcgtcatcac cgccgcgcag gacaccgcca
    23461 gcgcctccca gctcatccgc ttctcccgcc ccatcggcgc ggctttgcac ggcgattacc
    23521 tctccttcgg ctcgcgcacc ccgctgcgcg agatcatcca cgccgtgcaa aactcccacc
    23581 agccgctctt cccagtcata gacgaggaga ccggcagact ggagggcgtg ttttccaaat
    23641 ccgacctcgt cgaggtgccc cgcacccgcc tcgtcctcgt ggaccacaac gaattctccc
    23701 aggccgtggc cggcgcggat gaggccgaga ttttggaggt catagaccac caccgcctca
    23761 gcggcaacct gcgcaccagg gagcccgtgc gcttcatcaa cgagcccgtc ggcagcacca
    23821 gcaccatcgt cggcatcatg ttccgcatga gggggctcgc tccgaacaaa ggcaccgcca
    23881 tctgtctctg cgccggcctg atctccgaca ccctccacct tacaagtccc accaccaccg
    23941 gcaccgaccg cgagattttg gaatggctcg cccgcattgc cggcgtggac gccgcgcggt
    24001 ttgtgaagga cttcttcgcc gccggctccc tgctgcgcga ggccacgccc gcgcgcgcca
    24061 ttgagggcga ccgcaaagtc ttcgaggaaa acggctggcg catcagcatc agccagatcg
    24121 aggagatggg tctcgacgaa ttctggaaaa agcaggccgt gctgcacgat gccctcaccg
    24181 ccctctgcgc cgcccacagc ctgcacttcg cctgcctcat ggtcaccgac atcaccgccc
    24241 atcacagcgt cctgctcgtg gccggcgaca cccgcgtgga gcaggccata gacgccggct
    24301 accaccagcg caccccgcaa gtcttcgacc tgcccggcgt ggtcagccgc aaaaagcagc
    24361 tcttccccta ccttagcagc ctcgtcggca aactgacgcc gccttgagtc cacagggctg
    24421 acggcaagtg ccacggaccc atgccccatg atggcagatg ccatccaaac cttcctcaca
    24481 gcctgaaagc ctggcgcatg gcggcgcgaa ttctctccat gtcggcttct ggaagcacgc
    24541 cgagctttcg ctcaaacctg gctttgggca aagagccgat gccttgcaca tttgccacgg
    24601 actcggggga gaggaagcgc agatgcccga gggacacttc caagcaggag cctcgatttt
    24661 gcgaggtgat gggcacatgc acgatgaggc tgcgctcaac agggaactgg tcgtcgagca
    24721 ccacaacggc ggggcgtatc ttgcccgtca tgcccatgtc caccatccag acctcgccca
    24781 gtttcggaat catggcgcat cgaggagatc aagcacttct tgttcaactt cacgagaagg
    24841 aagcacgtcc tgccactcgg cctcctgctg ctggcgggcg gcaaagcgca tgaagtgcaa
    24901 tgcctcctgc agcacggatt caggcaggct gtcgatctcg atgaggatgg catcgcgggc
    24961 ggacatgctg tgagaataag cacgcatcgt gataaatcaa cttcacaccc ggcggtgctt
    25021 gagcttcaag atgccggcgg tttttgtgga atggaaaatc cccttgatga cgggttgcgc
    25081 gcgcagggct gctgctttta tctcgcggac cgaatcatgc cacacgcgcg ccttttccac
    25141 tcatgcctgc tgcttctggc ggcggctccc catgtccagg gccagcagga ggcgcccgcc
    25201 gagctgcgtc cgcgctggga ggcgggggcg cgctatgttc aggagaccct caccgagacg
    25261 gtgacggacc tctccgcgct gggcagcaaa ccggagcagg gcatgaaggt gaagcagacg
    25321 acgcgcatcg aggtgcgccc gggcggggag ggcggcaagc tggcgcgcgt gaccttcacc
    25381 gcgatgagcg gggaggccat cctggacggc aaaaggcaga cctttgactc gtccgacctc
    25441 gtctccgcgt cgccggagat ccgcgcctcc gtcgggcgca gcctggggca gagcttcgtg
    25501 cttgtgtatg acaaggagga ccgctttgtc gaagtcaggg actcctcctc catgctgccg
    25561 cccaccgagg acgggcagcc gaagctggag ggcatcgccg aggcggcgga ggtggcggag
    25621 cttttccgcc gctcgctgga gatgggcctg ccaaagacgg cggtgcgctc aggagaccac
    25681 tggacgaccc aggaggaggt gcgcttcccc agcgccggcg cggtgaaagt ggagttgcgc
    25741 gcgcagtacg aggcgcaggt ggactacctg gggcgccccc acgcgaagat cagcttcaac
    25801 ggcgtcatga agccctccga ggaaagcgcc ggcgccaggc cggtgacgct tggcgagggg
    25861 tcgaagctct tcggccaggt gctcttcgac ctcggtgcgg ccatgacctc cttcggcgcg
    25921 ttccgggcgg atttgaccct tgatgtcagg ggcaaaaaag tgcccgtgcg ccagcaggtg
    25981 accacgcgca tgacgcgcga gggctgaccc gcgccagagc cgcgcgcggg tcacccggcc
    26041 tcgaacaaac cgcgcgccca cggcgccagc ggcacatccg ccggggccag ctccaggcgg
    26101 tccagctccg ccggctcgat ccacgcgatc tcgtcgtgct ccagcgccgc cggctcgccg
    26161 gcgccggcct ccagcgcgca ctcgaaggaa tgcatgcgca ccgcgcacca gggatactcg
    26221 tgcaggatca ccggcccgcg ccgcagcacc cgcgcggcgc agcccagctc ctcgcgcagc
    26281 tcgcgcagca gcgcggcctc ggcggtctct ccgggctcga tcttgccgcc ggggaactcc
    26341 cagcaaccgg cgagcctttt ccccgccgga cgccgcgcca ccagcagacg cccgccgcgc
    26401 ctgagaacgg cgcagacgac ctcgacgggc gggcggtttt gatcttgcaa atccaaagcg
    26461 ccggcggcat agcgcggcca tgcgcccgga gaaagtgaaa ttcatgctgc tggccgcgga
    26521 catgcgccgc gccgtgcgtt tttattcgga gacactcggc ttcgaaaaag tcttcgtcag
    26581 tgattactgg tccgagctgc gctgcgggga cgccatcctg gccctgcacg gcgggcatga
    26641 cggttcagcc aatcccaccg gcctcagcct gcaatacgag gacgtttttg acgccgccgc
    26701 ccgcatcgag aaaagcggcg gcgccatcct cgaaatgccc aaccagcgcg agggcgagcc
    26761 catcctgctc ggccgcttcc gcgacacgga gggcaacgag gccttcatca cccagtatgt
    26821 cggctgagcc gtgacgatgc ggaagattcg tcgtgagagc gagcgaggac cgcgggagca
    26881 aggcggggcg cggatttcgg ccctgagtgt atgggtgcat actcgacggg tcgaaatccg
    26941 cgccccaacg ccgctcatgc gagtccgcag ccgctcgcag cagaatcctt ccgcatcgtc
    27001 acggctcagc ctcagcgcag gcgcgccatc gcgggctcgg ggtactcgaa ggtgcggccc
    27061 tcgaagttcc ggatcagcgt ccgctgctcg tcgcgcagca gcacatagtc cggattcacc
    27121 ggcaccttcc cgccgccgcc cggcgcgtct atgacgagct gcggcaccgc gtagccggtg
    27181 gtgtggccgc gcagttgctc gatgatctcc acgcccgcct gcacgctggc gcgcagatgg
    27241 gagctgccct ggatgagatc gcactgatac aggtagtagg gccgcacgcg gcacatcagc
    27301 agcttgtgga cgagggactt catcacctcc gcgtcatcgt tcacaccgcg cagcagcacg
    27361 ctttgattcc ccagcggcac gccgtggtcc gccagcctgc ccagcgccgc gcgcacctcg
    27421 gtggtcagct cgcgcgggtg gttcgtgtgg atgctcagcc acagcgggtg gtggcgctgg
    27481 agcatccggc aaagctctgg cgtgatccgc tgcggcagaa aaatgggcac gcggctgcca
    27541 atgcggagga actcgatgtg cgggatggcg cgcaggcggg agaggatggc ctccagcttc
    27601 gcgtcgctga acagcagcgg atcaccgccg gagagcagca catcccgcac ctcggtgtgc
    27661 ttctccaggt agcggaaagc cgcctcgtgg tccgtgtgca gctcctgctc ccccacgccg
    27721 gagaccacgc ggctgcgcgt gcagtagcgg cagtacgcgg cgcaccggtc ggtgacgaga
    27781 aagagcaccc ggtccgggta tctgtgaacc aggcccggca cgggcatgtg gctgtcctcc
    27841 ccgcacgggt cgctcatttc atccgggtct tcccaggtct cctcaatgcg cgggatgacc
    27901 tgccggcgga tgggatcctc cgggtccaga gggtcaatca gattgaaata atgcggcgtg
    27961 acggccatgg ccagcttcct gccactcagg agcacacccg cgcgctcctc ttccgcaagc
    28021 accagatgct cctccagtcc cgccagcgtg gtcacacggt tgcgcaattg ccacgccggc
    28081 gagttccaat cctccgccgg aacgttcctg ccggcccaaa atccgggagc atgagagcgg
    28141 aattccttct cgggaagcag ggtgggcggc gtgcgcatgt tcgactgtgt gcgaggttac
    28201 gaccgggaaa ttccgcctgt caaacttgtt gccgaaaaag atgcgccgcc cgccgcccgc
    28261 gcgcggcgcc ccccctcacg ccgccgccaa acagcttgcg ccgcgcgtgg aaaatccgca
    28321 tcatccgcgc atggcagacc ttcccaaaat ccacgacaaa aaacccgccg cccttgagat
    28381 cgaagccgga gaccactggt ggtgcgcctg cggcctcagc ggccaccagc ccatgtgcga
    28441 cggcgcccac aaaggcagcg gcatggcgcc gaaaaaattc acccttgagg aaaagcggaa
    28501 ggtctggctg tgcaactgca agcacacgaa gaacccgccc ttctgcgacg gcagccactc
    28561 cagcctggcc tgaaacccgc gcgcgcgtct gaggctggca aggcactgcc atccccgtca
    28621 tcccgcgagc catgccatgc gccgtcaggc tttgtgatcc cggcggatga gcggcgccgg
    28681 aggcggacgg gaaggtcaaa atttgaattt cgaatttcgg tttccaaccc gctacgaggc
    28741 cgaggggatg ccgaagctga agcgcagcac gccgtcaatg agcgccaccg acaccgtcgg
    28801 cgaatccccc ggcggcagcg gcgtcaccga gtccaccacc gcgccggtga tcaccggcac
    28861 ggcgtcaatg aggtctttga ggcccgtgag ctgggcgcgc atcacggcgc tggaaagggc
    28921 ggagttgttg gcgggttggg cggggtcgta gggcatggcg gggagaggga aaggggtgag
    28981 cggatgacac gatgaggggg tgacacgatg acaaggcggc agggtgaagg gggccgtgtc
    29041 acccggtctt cctgtcaccc agccaccctg tctccgaagc ggggactcaa agcacgcgga
    29101 tctgcgcggg gtcactccac gcgcccatga tgcctttgat gcccacagtg cgcacgcgca
    29161 cccagacgat cgtgcccgga gcgaagccca tgatctccgc gcgcccgccc ttgatgatgg
    29221 ctttctgcac ccagtccgcc tcattgttgg ggtcgcccgt gctggtctgc acctcgttga
    29281 cgctgccgcg cttctccggc ttgtaacggg cgatgaagcc gcccggcagc tcgctgtgct
    29341 tcaggcgcag gtccgcgggc gcgttcggcg gcgtggtgtc cggcgccttg tccgtgtcat
    29401 aaaacgggaa gccgcttttc tccacgatca ggccgtcccc cttggccacg atgttcacat
    29461 aaccgcccag atcccccagc gtctcctcca acagctcgcg cgccgcgttg aaggccatga
    29521 tatccgccgt ggcccggctg gcgcgcgccg agagcttggt catgtaatcg gtgatctgcc
    29581 cctgaaacac caccatcgtc acaggtggat cgtcaaaaat gccggcgttc agagtcatct
    29641 tgccgtggat ggtctgcgcc gtcgggccga gattgccgtc ggtgtaactt tggaagctga
    29701 tgactgcttt gatggtcgtg ctcataatgc tggatggttc cgccaactga gaatcccaaa
    29761 aaagccctgt gtcataatga acaggcatgt cccaggatag ttcagcaggc atggtggttg
    29821 atgtgcgggt tgatgtggct tgggttttgc cgcctcctca gcggcaaagg gttgcgcctt
    29881 cgctggcgac cgcctggccg ccagcgccgg agatgcggag gagccgcggg aaaacccgcg
    29941 ccgccacccg cctcggaaga aaaaatggcc ggatgccccg tcggaatgaa tcctccagca
    30001 tggattcccg cgcatcgcgc ctttcgtctg acgcggcata tcattcgtcc ggctcaaact
    30061 accgttattc tgatcaaaac taccgttcgt ctggcgcaaa ctaccgttat tttggtccaa
    30121 actaccattc gcctggcgca aactgccgtt atacaggacc aaactaccgt tcgtccggct
    30181 caaactacca ttattctgga ccaaactacc gttcgtctgg tgcaaactac tgttattctg
    30241 gaccaaacca cccttcgtct ggtgcaaact accgttattc tggaccaaac taccattcgt
    30301 ctggcgcaaa ctaccgttat tctggaccaa actacccttc gtccggctca acgcgcacat
    30361 cggccacccc aactggcgcg gcagagagat ccgcccgcct gaaagaagcc gcccatggac
    30421 attgccttga agaaggagcg caggacctcc cgccgcaggc accgcgcggt tttgcgcggg
    30481 cgggatggag tggggaggcg gattcatcgc agtgaaaaat caatttcacg cgcatatcag
    30541 cacaggaaac ggacaccgcg ttagccaaaa gaaattgggt aacgcaccgg ctccgggcgc
    30601 tcagcccaga gcctcgttca tgaactcctg caacgtcatg ccaagatggt agcagatgcg
    30661 cgccagattg cccgtcaggc agccccggtc gtgctttccc tgctccaggt tgaggatcgt
    30721 ctggtcgctc aggccgcatt tgcggcccag cgcgtaagcg ctgagcccgc gctccacgca
    30781 caagcgcagc agtgtccggc tgatccgttg atttacccac gcctcatact gcgcggccgt
    30841 catggagttg aatttcgggt ccatgccccg cattcaaaat caaacctgcc gccggacggg
    30901 ataccacttt gaggttatgc cctgcctggt gcttgagaca ggccggacgg gttggacgag
    30961 tcagacatgc cggacccgtc cggtggaacg cgcatcaccg aagaagccgg catcccctgg
    31021 aaaacacctc actcagcctc aagcctccca aacccttgca ccgcagagat cagaacaccg
    31081 cagagaagac ctctccgaat gcctctccga atgcctctcc gaatgcctct ccgcggtttt
    31141 gtttcctctc gcggtgtgcc cggtttggaa gcgctatagg ggactgctat ggagggacgg
    31201 acatttgggt tcgttctccg gcaaactcaa tgccccctcg acgccgcgca cggctcctgc
    31261 gcttcatcaa ggcggaggct ggttgtcgcc atgccgttcc atggcacgct cgagggcgga
    31321 gggcagcggt tgggccttct cggcggcata cacgcgggcg aactcctcca gcagttccat
    31381 gtccacgagg tcctttttgc ggttggaggc tttcttgctg gcgatgatgt cgcgcaggtg
    31441 ggccacgggg aagatgtcct ccatgatgcg gcgggacttt gcggcggtga aggactcgat
    31501 gccatcgggg gcgtgaatga tgtccacatc gaaggggccg tctttgattt gcacgaagtc
    31561 cttgcctgtg agcaaggctt gctcggtttc ggcatcaatg tggaaatcga gatcacgcaa
    31621 agccgcgatg cagcggcggg cattctcggc gtccttggga gcaaagagat cgagatcaag
    31681 cgtggtgccg ggatagccca gaaggatcgc gccagctttg ccgagaaaaa ggtagtcagc
    31741 cccatgttgg gccagcgctt cagcaagctc gcgggcctgg gcgagttcga acttgggagt
    31801 ccgctcaggc gcgtccatag ccaaggtagg agggctgatg ctgctcacac caagcccgat
    31861 actcgctcat ggtggcaaag gaacgattga cgtggtcgcg gttgcgaacg cgcacgaagc
    31921 catacttcag ccgcgcgctc agcgggcgct gcatctggcg gcggcattgc tcccgccaaa
    31981 gccggagagt ttcttcgtta gtctgtggca tggggggagc ttaatgcagc agatatgccc
    32041 ggcaagcccg gactcgtctt cccccctctc cctcaacttc cccctcgccg attcgctgcc
    32101 gcttgaactc ctcaatgacc ggcctcgctc ctcctccgct gaagcatcgg agcccaggcc
    32161 cgcgatgcct tcctcacgga cactgaatct ctggatcgcc ctcgcggtga aggtgctgga
    32221 agtcggagag atttcgcttg aggagaacat cagcctttcg caggcaccaa ccgggcctgg
    32281 gcgcggaaga tgcgatctcc cagggtcttt tcacgctcac gcagatagtc accgatcctc
    32341 tttggggtgt gatcgaagct gccggtgatt ttcccgcgga tttcacggag ccattcgagg
    32401 tcgtccaggt tcgtgtgata cttggcagtt gtgttcacga tacctggtgg ctgtggatct
    32461 cacggcgacg agtgcgaaga ggaccctttg aggaaatccg ttctggcatg ctgattctga
    32521 tttcagcgaa tgaacttttg ccaattgcag caggggtctt gcgggccagc agccattgca
    32581 tgatggactg tggagactcg gcggagcttt tgcgggccag caggccggaa aggctgatgt
    32641 cttcctccaa gtccggccag tgaatgccgc gccctttggc gatgagccgg tggtttctcc
    32701 gctcggcctg ggtggcgaag tgcagacgcg ggtagagcgt ggtggggacg gtaatttgac
    32761 gcccgtcact caaatgcacc cgcatcagag gcccttcgaa gcgcagcttc gagatggcgg
    32821 gttcctgatt gtcagtggtc gaagtactca tgccattttt gaatgatagc cgcttggtgc
    32881 tggatgacaa gacgttcgat ttttcgcagt tcgaccgggc cgaagtctcc tgcttgctat
    32941 aaagggacgg acacttcagt ttgctctgct tgctataaag ggacggacac ttcagtttgc
    33001 ttcagtttgg ctcccacgaa atgcataact tacggtttga cctgttaatc atgcgcccgt
    33061 ttcatgccgt ctccagactg ttgatttttg gccgcccgcc cgcggtctta tggaagcccg
    33121 cgtgcccatg tgcgaggatt tggattaact tccataatca tgtgtagcag catcatgaac
    33181 atcgtagaat cggcaaagaa attcgggtat gcttcacgaa tgccaccgac agaagctgct
    33241 gaaaccaaag caacaaccaa atcaggcttt gttgccgctt ccttttcgaa agtcgcataa
    33301 cgaactgcag cttcggataa agcatctttg gcgaaaatct gagaatttat ctgcttaagc
    33361 tgagtgtcga taaccaataa aatgtagcct gatttcggtg agttgccgat ttgattatct
    33421 ataactttga ttgagttagc atatgcttca aatttcttgc caattcggag ggttttaaac
    33481 ctctcctgaa cttgtgcgca ggaaataagt aaatccgggt tcctgtgaac tctctgggca
    33541 aaagtctcaa ccttctcaag gtcgggcatc tgttgaaatc caaagatgtc atccatgaca
    33601 gcgaaaagat cactgactcc ccgaaaaaag gccgtccact cagggtctcc ttggttggat
    33661 ttcaacgcct gccccgtgaa caagtcaaca atctcaaggg cagttgccca atagtgctga
    33721 aaacgggtgc gcagttgaat ctcaatattc ctcatttcac cacgttcatt tggaaatcta
    33781 cccaccaaat gaaagcttct atatccacta tctttggggt gcttgaggta atctttagcg
    33841 cgatacctcc cggcttctgt tttgaatgct tcgagctttt tgagatcgcg aacacacctc
    33901 aacagtttct tctcatttcc aacaatcgcc cgacagcctc caatatcttg catatttctc
    33961 agggtcatat tatcgatcct cttgagcttt ttataaatcg atgcataacg tttcagtctt
    34021 ttggcgaaga tcgcatcctt gtcgtgccgg agaacgactt gctgaagttc ctgccaagca
    34081 agatttagtg gtcgctcgtg gcataaccgc caataagata aaacatccat cacggcattc
    34141 aaatcctctc cctgagggtc tttgatgaga gtctcacctg ctttcgtgat ttgcttgttc
    34201 gagtatttca tgtgcatgac tcccctcaag gcttggcagt cgcctcacaa gcaagctaga
    34261 aacggacttt ctataaaatc aggttaggtc tgacatagct tcgaggttga ggcgcatcac
    34321 tggcacgcct cgcacgtccc gccgttgcgc ctagcttcga tggagcaggc ttggacttcc
    34381 tcggcggtgt attcgcgctt ggcgccgacg gcgccggcga tgacgctgct gcggatgtct
    34441 ttctgcacct ggacatcggc tttttcgatg ttggaggcgc tgacggtgcg caggtagtag
    34501 gtggtcttga ggcccttccg ccaggcgccg cggtacatgt ggctgagggt ggcgagctct
    34561 ttgccggaga agaagaggtt cacgctctgg ctctggtcta tccatttctg gcggcgggcg
    34621 gcggcgtcca cgagccagct ccagtcaatg ctgaaggcgg tggcgtagcg gcgcttgagg
    34681 tccacgggga tttcctcgat ggcgtcaatc tcgccgtcca tgtacttgag cttgttctgg
    34741 atgtcggcgg tccagaggcc gcgctttttg aggtcgcgga tgaggtaggg gttgagcacc
    34801 atgaagtcgc cggagaggtt ggacttgacg agcatgttgc tcatcaaggg ctcgatgcag
    34861 ggggaggagc ccatgatgtt gctgatggtg gcggtggggg cgatggcgag gacgttgctg
    34921 tttctcatgc cttgcttggc gatcttggcg cgcagggcgc tccagtccat cttgccgccg
    34981 cgggggacct cgacggggac gccgcgctct ttttcgagga gttcgagggt gtcctggggc
    35041 aggaggccgc ggtcccattt gctgcctttg taagtggagt aggcgccttt ttcggcggcg
    35101 aggtcgctgc tggcctcata ggcgtagtag gcgacggctt ccatgaattc g
//
LOCUS       DAOMYJ010000029        34434 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Prosthecobacter sp. isolate AMR_MDS_5629
            NODE_459_length_34434_cov_21.783327, whole genome shotgun sequence.
ACCESSION   DAOMYJ010000029 DAOMYJ010000000
VERSION     DAOMYJ010000029.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867850
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prosthecobacter sp. (wastewater metagenome)
  ORGANISM  Prosthecobacter sp.
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Verrucomicrobiaceae; Prosthecobacter.
REFERENCE   1  (bases 1 to 34434)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 34434)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.30
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:34:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,742
            CDSs (total)                      :: 4,684
            Genes (coding)                    :: 4,676
            CDSs (with protein)               :: 4,676
            Genes (RNA)                       :: 58
            tRNAs                             :: 55
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34434
                     /organism="Prosthecobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_459_length_34434_cov_21.783327"
                     /isolate="AMR_MDS_5629"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1965333"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..617
                     /locus_tag="PLP58_05025"
     CDS             <1..617
                     /locus_tag="PLP58_05025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012420343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HRJ07909.1"
                     /translation="WRRLEGGLWVAGVLLVVVALARCPYFEGFFREKLPAPTFGTGGI
                     LWASLTLALMTLPVVIVATEEALAAVPRGVREAALACGASRWQMIQRVALPGALPGVM
                     TGVILAMARGAGEVAPLMITGVVKLAPTLPVDLEWPFLHAERKFMHLGFHIYDLGFQS
                     PDSEAAIPMVFATTLLLILLVVVLNLAAIHIRSRLRARFQAAAF"
     gene            624..1379
                     /gene="pstB"
                     /locus_tag="PLP58_05030"
     CDS             624..1379
                     /gene="pstB"
                     /locus_tag="PLP58_05030"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004810563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HRJ07910.1"
                     /translation="MPASIQVEHFSFWYGRRQVLHEISMDIRPGEITALIGPSGCGKT
                     TLLRNLNRLNDLVDDIRHEGDIRVDGGSIFDPGLEVISLRKRVGMVFQKYNPFPMSIY
                     ENVAFPLRVAGQNDKAALDEVVERSLRGAFLWEEVRDRLHEGAPGLSGGQQQRLCIAR
                     AIANRPQILLMDEPCASLDPVATLRIEELMHELRREFTLVIVTHNMEQARRVADRTAF
                     FYLGRLVECRPTLELFLTPEDARTEAYVRGRIC"
     gene            1433..2962
                     /locus_tag="PLP58_05035"
     CDS             1433..2962
                     /locus_tag="PLP58_05035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ07911.1"
                     /translation="MISRSFGLLSAALIPVLPVLAEPPELKVAALEFGVAPEKGGFTA
                     SKVGALVREVAGPLTTVVTLFESGDKRVCLITPHMNSPKGVNVSPLIRRTAAEALAMP
                     VENVLLMVSHNHTDFNLVSNHLEALMTLSLKPEEIPAPQLVPAGEEFLQKLAATAKQL
                     PALLQPVTVWWAVGTENRISYNRKGRRADGSTYLMREEDRDLMGADFKGDVDEQAPIV
                     VLKNAQGSVVTAITHFTAHPCTCYHPEKPIVFGDWPQVACEIMAGHLSPETPVSVSFL
                     QGCAGEVNSKGMLRGDVELSKKYGRMLADSYIAALQDLKPSARGGLDFAIEKVRIPLA
                     PLPSEEVLKAEIAEMEDFITRANAGDENTRLCAGQNFPRELTPAYRGKLVEMILPWSR
                     WALDLRQSGRADTVAKFLEVDIQVLRVGDVGIVGMPFEPFQGIGRQIREGSPLPLALP
                     CGYTNISYGYLTDGPNTGDTEYMSANYRYSKFRPPYAKPAGDVIATQALETLRRFARQ
                     E"
     gene            2976..3632
                     /locus_tag="PLP58_05040"
     CDS             2976..3632
                     /locus_tag="PLP58_05040"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012360.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0120225 - coenzyme A binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HRJ07912.1"
                     /translation="MTDEPFHEIGAHAQVDAGVILGYRYPGDSQPARIGEHAIIRSGS
                     IIYANTRIGRRFQCGHQVLIRGEVVIGDRCVVHHQCTLEGRLTIGNGVKIMAHVYVPS
                     RTSIGDFVFIGPGTTFLNAKYPMREGTVQGATIEDHVCIGGGVTICPGVRVGRGSFIA
                     AGAVVNKDVPPGTLAMGVPARHQPLPPNIPPENLLEHLLPQTDLFGAQQDESWRDEDF
                     "
     gene            complement(3647..3916)
                     /locus_tag="PLP58_05045"
     CDS             complement(3647..3916)
                     /locus_tag="PLP58_05045"
                     /inference="COORDINATES: protein motif:HMM:NF021429.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VF530 family protein"
                     /protein_id="HRJ07913.1"
                     /translation="MHAAGPKNPLHGVTLEMMVTRLQAHYGWEMLGRMIKINCFNWQP
                     SIKSSLTFLRRTPWARQKVEQLYLDTLPDLRNGDADGGATGGSEF"
     gene            complement(3931..5400)
                     /locus_tag="PLP58_05050"
     CDS             complement(3931..5400)
                     /locus_tag="PLP58_05050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009959978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1501 domain-containing protein"
                     /protein_id="HRJ07914.1"
                     /translation="MFRIPGALGKDLCDKDLGMTRRDILRVGGASMMGLTLNGMFRAQ
                     AASANSAAAGRAGWGKAKHVLMIYLQGGPSHLDLWDPKENVPDKVRSAFKTIPTKVPG
                     HHFTEILPQLAKVNDKFTMINSMSYTPNGLFNHTAAIYQIMTGYTTDKVSPSGQLEPP
                     NAKDYPNFGSNIIRLKPMDEPMLPFVMMPRPLQESNVVGKGGTAGFLGKAYDPYTLYP
                     EGDDMDMGKMDRIKIEDLQLRPDLFSVRLQRRAKLRDAINDQMPTIEAAVKDLSLDSY
                     YDQALSLIASGRARDAFALEREPVKLRDRYGRNTFGQSCLLARRLIEAGTRVVELVWP
                     KVANSDNHSWDHHVGLTDRMKTRSGPMLDQGLSALFEDLDASGLLDETLVVALGEFGR
                     SPEKGVSTSGNGNSADGRDHWPYCYTAVIGGAGIKRGYIHGKSDKTGSAPVEDPVHPT
                     ELLATIYHAVGIDPETIVYNHLNQPRELVKAKPVTKLFA"
     gene            5682..6044
                     /locus_tag="PLP58_05055"
     CDS             5682..6044
                     /locus_tag="PLP58_05055"
                     /EC_number="4.1.1.11"
                     /inference="COORDINATES: protein motif:HMM:NF003947.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004068 - aspartate 1-decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006523 - alanine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 1-decarboxylase"
                     /protein_id="HRJ07915.1"
                     /translation="MLRTLLLSKIHRAAVTDASVDYEGSLAIDIELMDACGMAPYERI
                     LVSNMSNAKRFETYAIEAPAGSRTIQLNGAAAHLGKPGDRVTIMSFAQVTPEQMAAHQ
                     PKVIVLNERNEIVNHRGI"
     gene            6153..6941
                     /locus_tag="PLP58_05060"
     CDS             6153..6941
                     /locus_tag="PLP58_05060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ07916.1"
                     /translation="MSRPSTLSSVFERVYSLDVVFQPECWKLCGDAHCCSFARHKERF
                     RLLGRMGPPAQELPLLPGEYDFLHARGWTAQFHGHERKLAPFDFGPAVIHLDTITSTR
                     PHCACDHDTRTTVCRLYPLLPVFDTAGALVSTEPLGIYEELEILDGLAPACRLDSLPF
                     SQLNLYLELAALLGAHPVARFHLMAYRLVKRHVAERVRNEKNAGGRDAFAVFEAAYMR
                     RKLLDQETLKAALAELWEAFVSRHGADFTDALAELRKACPPGAS"
     gene            7007..8023
                     /locus_tag="PLP58_05065"
     CDS             7007..8023
                     /locus_tag="PLP58_05065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009958669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ07917.1"
                     /translation="MIARFLFLACLTFQFAAHGEDAGRFKLTARASELDPRAREHPEI
                     DFVFSKDGKPQDVQNASVDPRVPMRGKLVVWLMGHNDALFERLNSYGLHAIQVHYANK
                     WFGILKPEDRLARGRVRLEAATGRDVSAQLQIPQPDGMMERAFQFVKWLDKENPAGKW
                     GQFISGDGAGIRWDKVIISGSSHGSTTAARFAKEVAVDRVVMLCGPRDQEQDWQALPS
                     ATDPRRYFGFSHVLDGGWTGDHYCRSWELLGMHEFGPIVTVDNAAPPYENSRRLISAA
                     DVGGDAGKAHGAVTPGKSSPKADDGTLLYEAVWKYLYTHPVELVGQPGQPDPDCQKEH
                     PLPR"
     gene            8244..9863
                     /locus_tag="PLP58_05070"
     CDS             8244..9863
                     /locus_tag="PLP58_05070"
                     /inference="COORDINATES: protein motif:HMM:NF019843.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HRJ07918.1"
                     /translation="MFLTALKAKLAAMVVVAGLNTDDMPGISKDMMSTLENLAAAQKA
                     AAEDPGKGPDVNASFNEALAKVNELAQTGDKDAQYALAHWGVLSNSNLNEVVVLYRKA
                     AEQGQLLAKVELAQVLLQAGQQNEASVKEAVKLIQEAEAGDNKVARRLLANLHLGGVG
                     GVEASVEKAKALLEKGSAAGDGEATLGLSQLYTQGATGLPKDDKKALEMLIKASDQGN
                     PVAMSTYAARLFDGDPEGSSQLVKKDPEKAKKMFEDAAAKGFAAANRLLGAIYENGMG
                     GTAKDLKKAVEYYTKAANGNDAQALFRLGNYFEAGLTQGEGEKAEIVIQKNDKSALDL
                     YRLAAQNGLPEAFFNVGVYYETGTVVDKDPEKAYNFFLRAANNGLAQAQFRLAGLYQN
                     GTGVAQDVVAAKAWYQLAADRGNVAAQIALGQMYEAGVGGPANIEAASQQYTNAAEAG
                     APLAMLRLASLAERGLNTAKNVPDKARALAWADLAVDASSNAELAVKYRDELKKTLKT
                     DEIAEAKKIYDTLKKSSSEAAPKGGESKSTR"
     gene            9954..10029
                     /locus_tag="PLP58_05075"
     tRNA            9954..10029
                     /locus_tag="PLP58_05075"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:9987..9989,aa:Ala,seq:ggc)
     gene            10139..11752
                     /locus_tag="PLP58_05080"
     CDS             10139..11752
                     /locus_tag="PLP58_05080"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF012492.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HRJ07919.1"
                     /translation="MSHSKPTEPKKKKSLLGHLKAGLKKIIGLGKKKTSAAEPKHADG
                     RHPAKMHPHARPPREDHTDGDSRPPRGPRREREGGRERKPRRESENRPPREERGERKR
                     EGRGPRGGRERSAEPRAPIRNFEPLPPPPPPPVPEGPYAPAFENLGLAPAVLAGIRES
                     GYETPTNIQSQAIPVVLEGRDVVGASQTGTGKTAAFALPTLSRLGAPGKFRCLVLEPT
                     RELAAQVVEQFEKYGRHTGQRVLLVHGGVGYEKQRRGLQEGVDIVVATPGRLLDFMQD
                     GTARLDDVEVLILDEVDRMLDMGFLPDVRRIVERTPKSRQTLFFSATMPPQIKTLADF
                     ALKDPVSIEIGIRFSPAETVSHFMYPVAGDQREELLLAILKQTHFNSMMIFTRTKMQA
                     DQLYGAIRQTGEYKVAVMHSDISQKDREKALKGFRDGEFEVIVATDLAARGLDISGVT
                     HVINFMVPENSEDYVHRIGRTGRAQKEGDAFTLFSAEELPYVASIERLINQKIPRRKL
                     GGFSYKYTTVLDEEDKARAILHGRKKKRR"
     gene            11783..12475
                     /locus_tag="PLP58_05085"
     CDS             11783..12475
                     /locus_tag="PLP58_05085"
                     /inference="COORDINATES: protein motif:HMM:NF019242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1573 domain-containing protein"
                     /protein_id="HRJ07920.1"
                     /translation="MNRRPAILALCLLSAAWCRAELALETPVIEVHPKPEDEQVTVHF
                     AFTNKGSKPVHVLTLDSSCSCLSADLDKPVYQPGEKGSGKAVFKVSSFTGRHEKTIHA
                     KTDDPAQPEWVIPFMLIVPEIIKIEPKTLQWWLGDEPQAKVTRVIMSGDEPLRITNIS
                     STRENVEFSFKEVQPGREYEVTVKPKNTQEVMLGALRIETDSKIAKYQRQLAFFSVYR
                     KPASLDATPPAP"
     gene            12472..12909
                     /locus_tag="PLP58_05090"
     CDS             12472..12909
                     /locus_tag="PLP58_05090"
                     /inference="COORDINATES: protein motif:HMM:NF012790.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HRJ07921.1"
                     /translation="MIRAVFQAFILGGMTLLAAAATHLLHPRAPALYLQDSPLREHEI
                     TLQQIEERWQNKVLWLDARPDEQYQEDHIPGALQLNEQKFDEHLLALLDTLQTTDMPV
                     IIYCGGEKCEASHRVREKLTPLVPLDTCLILKGGWPAWKAARQ"
     gene            12976..14466
                     /locus_tag="PLP58_05095"
     CDS             12976..14466
                     /locus_tag="PLP58_05095"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I adenylate-forming enzyme family protein"
                     /protein_id="HRJ07922.1"
                     /translation="MPTPALPHDWLAQAARQWPDTVFLEDEGISLTFAESLARVEALA
                     GWMRAQGVEKGARVLVCAPNRVELPLVAFAALRLGALFSLLSPQLQPAGLARILAQCE
                     PALVLLDAPTAHLRETAAASTARVHLLEEIPATGPAEPPAALTPADAAFLVFTSGSTG
                     TPRGVILTHGNVAFVAPVIQARLGYEKGDCIGVFLPLSFDYALYQMFYACLAGATLSL
                     ARPEMAGPELPGVLARRGITVLPGVPTLFAALLKMQRHRPRPLPALRMFTNTGDHLPQ
                     AHISQLRELFPHARVFPMYGLTECKRVSILLPEELDAQPASVGRPLDGTTVFAVDAEG
                     RRLPPGELGELVIQGPHLSPGYWRAPEETAKRFRHLDGVPTLFSGDQGRVDEAGFITF
                     HSRDDFLIKHRGTRLNPAEVEDAACALPGITGAGCVKDEPRGLLRLYLTTQGAPPAAA
                     EVLSALATVLEQAKLPDRIHPIPELPRTANQKLDRKALRALAEAEA"
     gene            14693..15370
                     /locus_tag="PLP58_05100"
     CDS             14693..15370
                     /locus_tag="PLP58_05100"
                     /inference="COORDINATES: protein motif:HMM:NF024751.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HRJ07923.1"
                     /translation="MKVTGGEAHFAHLPTVNLGCDALYLKELAASDAEAVHVVIRDNA
                     GFHLRDGDPRLPERVRIVALPPHSPERNRCEHAWDMIKDEVSNQCHRITHHAQQLRGL
                     ACVHRLAVALICQRFASDEIHRVVVLSLVPAKLVDAHDVRVFQVCRRLRLRLKALHLV
                     RARQHATADHFHRHHAAQLHLLRLPHHAHAALVDFFEQFITARIPHARSFRPRRRFEF
                     HGVKIGR"
     gene            complement(15732..20210)
                     /locus_tag="PLP58_05105"
     CDS             complement(15732..20210)
                     /locus_tag="PLP58_05105"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF019334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HRJ07924.1"
                     /translation="MNPDADLQTIRTQPPTPPVRFATGGEPAQGSANYHLGREVARGG
                     MGCILEAEDRKLARTVAAKAVLLDSDMDEGTKQRFIREAEVLARLSHPNIVPIYDIVW
                     EDGLPLFYTMKLVKGRTLQHILNELCNEHPEDLRDFTLDRLLLIFRKVCDAVAFAHSK
                     GVLHRDLKPENIMVGEFGEVLVMDWGLAKVLNDEVRESAASDSSFTAPSGASPTATLQ
                     GAVMGTPKYMSPEQAMGQIAELDERSDIFSLGGILYAILTLRPPVDGKDLHEILEKVK
                     TGGITPPTAFGVTTGAHARHVKGEVLEAKKIKPLPHIESGRVPAALSAVAMKALALDK
                     AKRYQNVAAFSADIERYQTGFATSAENAGFAKQAALLIKRNKGIFTTAFAAWLVVTAL
                     AVWFVFNLKAKEQRATEAEAVAVQEKETARQALAKSQIDLAEKEFERGKFIEAQKILT
                     ETPESFRDANWRFLQAHSSDFTARLSLPGKGAALRLQFLPQGDRFAARCSSGVIGIFS
                     LTGRQIGDSVPVNGSGAFRIDSAGGRIAFAASASEVAVQEVATGKLVHRWTCELGENK
                     HVLLSPDGATVLVAGGKQLIVYAAQTGAPLWTQPFNYVVPAFSPDGRTVAIVAAKTAL
                     ALKIELRDTLTGTVRSTIEASADNDGKEALQFNQAGDRLACLGRDEVILWNPQTATKI
                     RGLHFPGETVKLISPGGDAVATISGSRIRLWDTATGRLLRSFNGAGTDVVDLAFSPDG
                     RMLLSTHVAAENAILHVWPTRRGEEVAAVRQGGICSRVLFDRDGSTLCTSAQNAVAWN
                     SRSGVEKWTFSAGYKYRALDLALHPVDGSVVLSAIYIKEFIHLSSTGKALAPFGINYG
                     SSVKFNRDGRLLLEVEGAFHQTYPGPAFSVVEYPTGKVLRRIPFENPRQPFAAFCLDD
                     AAVATAAPTGGITVWDWQAGTPLRQIAAAQTGSIACLASSPDGRRLASGGPDRWVRVW
                     EAATGRLETAFRAHWEGVRCVKFSPDGREILSGSSLGTVRLHDAATGEERLAFYGLTM
                     PVVDADISADGRLITAIATDGDTKVWDRQRSSAAALLPKRPAVTKPPVAKDAEGWEDL
                     LAPLTPNEVEQTGNGWSLEDGELFSPNAKHATLPLPGNVAGSSYTVRVKLRQLATKQV
                     FHVVLPVADRMCGFELDGRPHGGIYTGLILVNGKFGKDLPGTVQGQQVNDSKPHDLQV
                     TVRLDGANATITTTLDAHPLYEWTGPTAALSQHEAWATTEPGALALGAHFGGWAVSGV
                     KVKRLDGSNAPAPGQPTMPAPTAPPSADETAKALSDVVYSALKDASLLVWFGKDQEHA
                     AASQRMLESAANTESYETAERVAKLVSLRPIEDAATQETVLDLARKAVELGQDAKTFL
                     PWANLTLGMAEYRGGHYPQAAIALGTTIQTAAETNYSSNQPRIEVTASFYLSMCLFRQ
                     GKPGEARALFTATEAKMKPLPADEQNPLTDGANHDDLILWLACKEAKALLAGAEKK"
     gene            complement(20258..23566)
                     /locus_tag="PLP58_05110"
     CDS             complement(20258..23566)
                     /locus_tag="PLP58_05110"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WD40 repeat domain-containing
                     serine/threonine-protein kinase"
                     /protein_id="HRJ07925.1"
                     /translation="MNPDTDTPTTRIRPPVRPVPVTTGGEPALGSANYHLGREVARGG
                     MGCILEAEDRKLARTVAAKAVMLDSDMDEATKQRFIREAEVLAQLAHPNIVPIHDIVW
                     EGGVPLFYTMKLVKGRTLQAILKDLRHQNSGVLREYTLDRLLAIFRKVCDAIAFAHSK
                     GVLHRDLKPENIMVGEFGEVLVMDWGLAKMLDDECRMTNARAPEPAAPDSSSGIRDSS
                     FSGTLSGALLGTPQYMSPEQAAGETNLLDERSDVYSLGAILYAILTLRPPVAGKSVEE
                     LLEKVKTGSITPPTSYGTTKSEAVAPKKNGVAEAKKLQPPPHLPSGRVPPALSAVAMK
                     ALTLDKTKRYQDVAALSAEIERYQSGFATTAEHAGTLKQLGLLMLRHKAVTALLGVMV
                     LLSIGFVARLVASEKRAIAGEQSAVAEAARASQAEAVAIQEKQATVSALARSALSLAE
                     AALREGNGSMMQAALGDVPEDLRDSTWHYLRGQSDSSIARIDTGTDIDDVVAHPKLPS
                     VFVVVDHDGIVTLLNVRTGKRLLEFSTGSEFPAIRFKAYRIAISPDGERIAVGRWQVR
                     SDHVGITIHDARNGKPLRQWDAARTVALAFGPDGKSLAQAAVDSEGARTIKFWDVESG
                     RLQWTYAELVGEGRNLTMNLAPGGQQLWVQSRGGAVLLSLNDGSVVRSVRLLNTWALV
                     LRPDGQVLVTGDARGAVKGYDPTDQHIVFEFRTDNRPIEHILFTANGSRLVTIAKLDD
                     GRQALRIWDATTGAPVQSLLGGSGDVTGACLHPLTGELVVTGPETRAWSLGMARWVLR
                     GVREAGVAFWGEDDLGFFPDGRKAATLQRLGSATPNSLWSASLADYFSISLSADGRFA
                     TVGTKRRNDPLLLLHKDGMNVKASEIPDVPWRESHRLCPTGKRVAIVWHNFEKAEVKD
                     TATGVSLPRLNGGNFIRFGDVGWLNDERLAGLVTAHAPRGEKGSEEQIVVWDITTGEA
                     LYRKTNPGQMDLLAVSPDARRIAEAGADKKVRIRDAATLEVLNEFRAHDGPITALAWH
                     PKKPILATGSTDLTIRLWNLDTGKRIDELRGPLAAPHTLAFAPSGQRLGCASLDATTR
                     IWDPPSLADTEAAK"
     gene            complement(23612..24373)
                     /locus_tag="PLP58_05115"
     CDS             complement(23612..24373)
                     /locus_tag="PLP58_05115"
                     /inference="COORDINATES: protein motif:HMM:NF019265.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HRJ07926.1"
                     /translation="MVIRQPEMSSPDPNASDSSFPPTQWTLVRAVQSGDPADAEAAMS
                     QICQRYWYPIYAYLRRSRHSAPDAEDLTQAFFMRLIKRETLQIIQLDGGKLRSFLLGS
                     LKHLLSDEARHHNAQKRGGGQKLLSIDEMQAEERYACEPQDMRDPEWLFTHAWANDLL
                     AGIREKLRAAFAVAGRAEVFETLLPFLMWDEEPPSHREIAQKLGCSEAASRVQIMRLR
                     SKFRALLEEELACTVLTPEDIPGELAWLRSVLAAK"
     gene            24482..26845
                     /locus_tag="PLP58_05120"
     CDS             24482..26845
                     /locus_tag="PLP58_05120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ07927.1"
                     /translation="MKHSAPSHLTVCILTFVMLHSIFVIPLPAAVPQLLNYQGRVAVG
                     TVNFDGTGAFKFALVNTDGTTTYWSNDGTSTAGSQPTAAVALTVTKGLYSVQLGDTTL
                     ANMTTVPASAFANADVRLRVWFDDGVNGSQLLTPDQRITAVGYAMMAGEAQTVADGAI
                     TGAKIAAGAVGGSQLGAGAVTSAKIAVGAVGSGALSSNAVNTSSIADGSILNADISNS
                     AAIDATKIGSGSVSNTMFGYLNGVTAPIQFQISNVLNGATSGTYTGSFSGAHTGNGSA
                     LTSLNGGSLQPGSVGSDSIADSSIANADISNSAAIADTKLATISTAGKVANSATTATT
                     AGTPNSIVLRSASGIIVGTIDGGGLISGSVSGTSIANGSITDSKLATISTAGKVANSA
                     TTVTTAATPNSIVQRDGAGLIPGTFNGSSIVSGSIGSSALSSSTVNTNNIADGSIFNV
                     DIASTAAIDAAKIGSGSVSNTEFGFLNGVTAPIQSQFSNVLSGAQSGSFTGSFAGTHT
                     GNGSGLAGISATNFSGSLAGDVTGTQGATVVSAVAGVTAADVAAGATLANEARVANIA
                     NTLVKRSAGGNFSAGTITAENLNVSGTVTATVIQGDGSGLSGVTASALSSDGVNAVLS
                     VLAARAQAKADALTAIIPTLGPAITQKQADITALTTQISSLNAQIQMIEVQRSVAQTN
                     YDEQRDDAIFDYTWGNPLNQPNPSDPTTWVPIHTQAEINAFIAAYPPFDGSSFDQQVA
                     NVNATKAAAQATKTTAEQELAALQTQLENVNIDLRAALTLADIAGGL"
     gene            26872..27327
                     /locus_tag="PLP58_05125"
     CDS             26872..27327
                     /locus_tag="PLP58_05125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ07928.1"
                     /translation="MKTLLLSLATLIPAAVHADPRSSANYTQPAEVSDAGGRRTTSAT
                     YANDGSVGFVTGISIAAAPAQTAKAGYIGQLYDVTGLALTSDAPEVEETKRLQLAAWQ
                     TLDDATFLSVLATDVTWSVQSGPLVNVSAAGKAKADVVYEDTSAVAQAV"
     gene            27358..27768
                     /locus_tag="PLP58_05130"
     CDS             27358..27768
                     /locus_tag="PLP58_05130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ07929.1"
                     /translation="MLDTIADNFGTYAGDGLGDDWQVQYFGPNNPNAAPGFISDGSGL
                     TNLFKYTAGLAPNDAASTFILNNTPVTNQPGKQQITLGPTFSDRTYTIEFSLDLKNWH
                     TLGPAFPGNGGTQIITDTNASGPHKFYRVSVNKP"
     gene            complement(28003..28269)
                     /locus_tag="PLP58_05135"
     CDS             complement(28003..28269)
                     /locus_tag="PLP58_05135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ07930.1"
                     /translation="MEKLRAEFAAKKDESTFDALKGTLSGAETSREELAKKLGMSEGA
                     LKVAVHRLRQRYREVLRAEIAETVNSPGDVDDEMRHLVAVLRSS"
     gene            complement(28282..28734)
                     /locus_tag="PLP58_05140"
     CDS             complement(28282..28734)
                     /locus_tag="PLP58_05140"
                     /inference="COORDINATES: protein motif:HMM:NF016430.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor"
                     /protein_id="HRJ07931.1"
                     /translation="MNDSIAPGAREFHTTRWSVVLAAQHGGDGTRALKAMSELCRDYW
                     CPLYAFVRRRGHAPHDAQDLTQAFFATLMETNATAADPRRGRFRSYLLGALKHFLAND
                     FHRANAQKRGGGRQIVEWDALEPEARYSLESADPIDADVIFDKRWAME"
     gene            complement(28806..29666)
                     /locus_tag="PLP58_05145"
     CDS             complement(28806..29666)
                     /locus_tag="PLP58_05145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ07932.1"
                     /translation="MKTLLRTRLITALLLIGMGSPIFAAEPIDKGQRVFSAGHSFHMF
                     MPKMLAELAKAAKIPDHQQAGISSIGGSYVYQHWNVADEKNTLKTALRAGQVDVLTLS
                     PIYLPDDGIENFAKLGFEHRPDIRVTIQEFWLPFDVFDVNYKKKKPEPVDRNSRTVAE
                     LRSINEPYYKSMDDHVRALNEKYGKQVVFVVPVGQASLALRAKIIAGEAPGLKQQNDL
                     FTDAIGHATAPLQWLTAYCHFAVIYRRSPIGLPCPAALGKEAHGEALNRLLQELAWDA
                     AKAHPLSGLR"
     gene            complement(29681..30265)
                     /locus_tag="PLP58_05150"
     CDS             complement(29681..30265)
                     /locus_tag="PLP58_05150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ07933.1"
                     /translation="MKLACFFLLFTSLLQAQEVLKAVKDRTQRGTLSDQRQLVRGDTG
                     KWNDAAMLLHTTKPQGMPLDDWMDEAGEKKPAVTAADENWLLFRTRQLDDNDRVWIEK
                     IERSGNAFTITMHEAIWQGNYFKTFTWYEVTAVNLGKLPAGDYTVTWLVKPLVFKQLE
                     KPREAQNNYQTNWPVDDQPGEKKPVELKTKFTVQ"
     gene            complement(30272..31582)
                     /locus_tag="PLP58_05155"
     CDS             complement(30272..31582)
                     /locus_tag="PLP58_05155"
                     /inference="COORDINATES: protein motif:HMM:NF024753.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HRJ07934.1"
                     /translation="MKAAFILLALAASVWAAETPNWPQFRGAAADGLGEGATLPETWS
                     DTENVVWKVKVPGWGWSSPIVWGDKVFVTAAVGEKELPTPTVGGYPGGHVGTGEVHRW
                     MLYCFDFESGKMLWEFEANKGIPPQMRHPRSSFANETPVTDGERVYAYFANIGLFCCD
                     MNGQKLWERRWPSYPMRDGWNTGTSPVLHGGRIFIQNDNDQESFLETVDKLTGKTLWR
                     VSREERSTWSTPYAWESGQRTELVTIGLNKVRSYAVEDGALLWEISGTAGLVSQIPLS
                     KHGLLYAGAGYHYGPLYAVKPGAKGDISLTGDATSNEFIVWSQKRGSSIHPGYLISGE
                     RLFVCYDSGLLGCFNAKTGEEIIPKTRLNTKGGRFYASPWSYNGRVFMLNENGDTWVI
                     EDGPEYKLIRKNSLGDVAWATPAIARGSLFIRTYSWLYRFQNKK"
     gene            complement(31687..32640)
                     /locus_tag="PLP58_05160"
     CDS             complement(31687..32640)
                     /locus_tag="PLP58_05160"
                     /inference="COORDINATES: protein motif:HMM:NF025748.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK motif protein"
                     /protein_id="HRJ07935.1"
                     /translation="MADILPAFASLTPSPHDGTAAVSYFALRIADSSRNYIAKTQQGH
                     PVLVIETRLPVGAYPPSLKLENLTVFHGYAGTMNTPDGVVTGVFSIVECRVSDVALIE
                     SFLRLAGHLLNGLSELPEPRAVAIEVRKLVQIFQALRQPAVKSIQGLWAELFVLANSP
                     DVPRWSLGWHTDPMERYDFAMQRLRVEVKSSANRVRRHHFSHEQLHPPSGIELWIASV
                     FVERSSGGINCIELLREIQSELSADAAFAVEAKLLEALGSDFSKVQDNRFDAALAADS
                     LSFVSVSAVPRMAEDLPDGVSELRYAVLIPQSASLQRQWPN"
     gene            complement(32652..>34434)
                     /locus_tag="PLP58_05165"
     CDS             complement(32652..>34434)
                     /locus_tag="PLP58_05165"
                     /inference="COORDINATES: protein motif:HMM:NF022063.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Z1 domain-containing protein"
                     /protein_id="HRJ07936.1"
                     /translation="PDRKERERLNQIFEDWRDHDTPAHERQFVLITVKKNWSVLRKLI
                     TLLNNVDRSGIPALIIDDEADQASLNTRVRQQEESTTYARLLQLRRLFPNHSFLQYTA
                     TPQAPLLISILDTLSPDFAELLNPGADYVGGIEFFSGAASRIHPILDVAPQAPLVAPP
                     ESLIDAMRFFFVGVAAGTVEGGRGNRSMLIHPSRETDPQGDFAHWVRLIKDRWVSALE
                     LPETEPDYADEIHAFQAAYAELATTVGSSLPPWDAVRAVLKRAIRKTNISEVNSTSRR
                     ADVDWRAGYAWILVGGQALDRGFTVEGLTVTYMPRGLGTGNADTLQQRARFFGYKRRY
                     IGFCRVYLDPVVQDAFADYVTHEENVRLQLNEWKQTGRPVQEWKRAFLLGRNLHPTRR
                     NVLSLDHVRDSYADEWFWVRSPHESDDAVTANRNLFDSFVAGHAFVDLPENVHRTHAR
                     QHRMAAGIPLQEVYEGLLSQVHVRAPDDGQKYFGALLQIHQHLQGHPNELCTVYQISP
                     YVPSSRGIDSQTDEIKQLFMGSNGNPGDQNYDSGQRHLHADGQLSIQLHRLNLTDNDA
                     PLVNDVPVIAVWVPAVMAQDWLVQDNS"
BASE COUNT         6440 a        11129 c        10531 g         6334 t
ORIGIN      
        1 catggcggcg gctggagggc gggctgtggg tggcaggggt gctgctggtg gtggtggcgc
       61 tggcgcgctg cccgtatttc gagggctttt tccgtgagaa gctgccggcg ccgacctttg
      121 gcacgggggg aattttatgg gcgtcgctga cgctggcgct gatgacgctg ccggtggtga
      181 tcgtggccac ggaggaggcg ctggcggcgg tgccgcgcgg ggtgcgggag gcggcgctgg
      241 cgtgcggggc gtcgaggtgg cagatgatcc agcgggtggc gctgccgggg gcgctgccgg
      301 gggtgatgac gggtgtcatt ctggcgatgg cgcgcggggc gggggaggtg gcgccgctga
      361 tgatcacggg cgtggtgaag ctggcgccga cgctgccggt ggacctggag tggcccttcc
      421 tgcacgcgga gcggaagttc atgcacctgg gcttccacat ttacgacctg ggcttccagt
      481 cgccggactc ggaggcggcg atccccatgg tcttcgccac gacgctgctg ctgatcctgc
      541 tggtggtggt gctgaacctg gcggccatcc acatccgctc ccgcctgcgc gcgcgctttc
      601 aggcggcggc tttttagaaa accatgcctg cctccatcca agtcgagcat ttctccttct
      661 ggtatggccg gaggcaggtg ctgcatgaga tctcgatgga tatccgcccc ggtgagatca
      721 cggcgctgat cggcccatcg ggctgcggga agacgacgct gctgcggaat ctgaaccgcc
      781 tgaacgacct ggtggatgac atccgccacg agggggacat ccgcgtggac ggggggagca
      841 ttttcgatcc ggggctggag gtgatctcgc tgcgcaagcg ggtgggcatg gtcttccaga
      901 agtacaatcc ctttcccatg agcatctatg agaatgtggc ctttcccctg cgcgtggcgg
      961 ggcagaatga caaggcggcg ctcgacgagg tggtggagcg cagcctgcgc ggggccttcc
     1021 tgtgggagga ggtgagggac cgcctgcacg agggcgcgcc gggcctctcg ggcgggcagc
     1081 agcagcggct gtgcatcgcg cgggcgatcg cgaaccggcc gcagattttg ctgatggacg
     1141 agccgtgcgc ctcgctggac ccggtggcga cgctgcggat cgaggagctg atgcacgagc
     1201 tgcggcggga gttcacgctg gtgatcgtga cgcacaacat ggagcaggcg cggcgggtgg
     1261 cggacaggac ggcgtttttt tacctgggcc gcctggtgga gtgccggccg acgctggagc
     1321 tgttcctgac gccggaggat gcgcggacgg aggcgtatgt gcgggggcgg atctgctgag
     1381 aaaaagggag attgcgggag cgaagcggcg gcggcggcgc gtttcctgcg ccatgatttc
     1441 gcgctcgttt ggtcttttgt ccgccgcttt gatccccgtg ctgcccgtgc tcgcggagcc
     1501 gcctgagttg aaggtggcgg cgctggagtt cggggtggcg ccggaaaagg gcggctttac
     1561 cgcgagcaag gtcggagcgc tggtgcgcga ggtggcgggg ccgctgacca cagtggtcac
     1621 gctctttgaa tcaggagaca agcgtgtctg cctcatcaca ccgcacatga actcccccaa
     1681 gggtgtgaac gtcagcccgc tgatccgccg caccgccgcc gaggctctgg ccatgccggt
     1741 ggagaatgtg ctgctcatgg tctcgcacaa ccacacggac ttcaacctgg tgagcaacca
     1801 cctggaggcg ctgatgacgc tctcgctgaa gccggaggag atccccgcgc cgcagctcgt
     1861 gcccgccggg gaggagttcc tgcaaaagct ggccgccacg gcgaaacaac tgcccgccct
     1921 gctccagcct gtgacggtgt ggtgggcggt gggcacggag aaccgcatca gctacaaccg
     1981 caagggccgc cgcgccgacg gcagcaccta cctgatgcgc gaggaggacc gcgacctgat
     2041 gggcgcggac tttaaaggcg acgtggacga gcaggcgccc atcgtggtgc tgaaaaacgc
     2101 gcagggcagc gtggtcacgg ccatcacgca cttcaccgcg catccctgca cctgctacca
     2161 cccggagaag cccatcgtct tcggcgactg gccgcaggtg gcgtgcgaga tcatggcggg
     2221 gcacctttcg ccggagactc ctgtgagcgt cagctttctg caaggctgcg cgggcgaggt
     2281 gaactcgaag ggcatgctgc ggggcgatgt ggagctgtcg aaaaaatacg ggcgcatgct
     2341 ggcggacagc tacatcgcgg ccttgcagga cctgaagccc tcggcgcgcg gcggactcga
     2401 ctttgccatc gagaaggtgc gcatcccgct tgcgccgctg ccctcggagg aggtgctcaa
     2461 ggcggaaatc gccgagatgg aggacttcat cacgcgcgcg aacgccgggg atgaaaacac
     2521 gcgcctgtgc gcgggccaga acttcccgcg cgagctgacc ccggcctacc gcggcaaact
     2581 ggtggagatg atcctgccct ggagccgctg ggcgctggac ctgcgccagt ccggcagggc
     2641 ggacacggtg gcgaagtttt tggaagtgga catccaggtg ctgcgcgtcg gcgatgtcgg
     2701 catcgtcggc atgcccttcg agcctttcca gggaatcggc cggcagatcc gcgaggggtc
     2761 tcccctgccc ctcgccctgc cctgcggcta caccaacatc tcctacggct acctgaccga
     2821 cgggccgaac accggcgaca cagagtacat gagcgccaac tatcgctaca gcaagttccg
     2881 cccgccctac gccaagcccg ccggggatgt catcgccacg caggcattgg agacattgcg
     2941 gcgctttgcc aggcaggagt gaaaccgcca tgcccatgac cgacgaacct tttcacgaaa
     3001 tcggcgcgca cgcccaagtg gatgccgggg tcatcctcgg ctaccgctac cccggagact
     3061 cgcagcccgc gcgcatcggc gagcacgcca tcatccgcag cggcagcatc atttacgcca
     3121 acacgaggat cggccgccgc tttcaatgcg ggcaccaggt gctcatccgc ggggaggtcg
     3181 tcatcggcga ccgctgcgtg gtgcatcacc aatgcacgct ggagggccgg ctcaccatcg
     3241 gcaatggcgt gaagatcatg gcgcatgttt atgtgcccag tcgcacaagc atcggtgact
     3301 ttgtcttcat cggccccggc acgacgtttt tgaatgccaa atatccgatg cgtgaaggca
     3361 ccgtgcaggg cgcgaccatt gaggaccacg tctgcattgg cggcggcgtc acgatttgcc
     3421 cgggtgtgcg cgtcggacgc ggcagcttca tcgccgccgg cgccgtggtg aacaaggacg
     3481 tgccgccggg gacactggcg atgggtgttc ccgcgcggca tcagccgctg ccgccaaaca
     3541 tcccgcctga aaacttgctg gagcacctgc tgccgcagac cgacctcttc ggcgcgcagc
     3601 aggatgagtc gtggcgggac gaggactttt gaaacagacc gtttgttcag aactctgagc
     3661 cgccggttgc gccaccatcc gcgtccccgt tcctcaaatc aggcaaagtg tcgaggtaga
     3721 gctgctcgac tttctgccgg gcccaggggg tgcggcggag gaaggtgagg ctggacttga
     3781 tgctgggctg ccagttgaaa cagttgatct tgatcatgcg tccgagcatc tcccagccgt
     3841 agtgggcttg aagccgggtc accatcattt cgagggtgac accgtggagg ggatttttcg
     3901 gaccggctgc gtgcatgagg cgcgcgtctg tcaggcgaaa agtttcgtca caggcttggc
     3961 cttcaccaac tcgcgcggct ggttgaggtg attgtaaacg atggtttccg ggtcaatccc
     4021 aacggcgtga tagatggtgg cgagcagctc ggtggggtgg acgggatcct cgacgggcgc
     4081 tgagccggtc ttgtcggact tgccgtgaat gtagccgcgc ttgatgcctg cgccaccgat
     4141 gacggcggtg tagcagtagg gccagtgatc gcgtccgtca gcactgttgc cattgccgga
     4201 ggtggacacg cctttttccg ggctgcgtcc aaactcgccg agagcgacga caagtgtctc
     4261 atcaagcagg ccggaggcgt ccaggtcttc aaagagagcg ctgaggcctt ggtcaagcat
     4321 ggggccggag cgggtcttca tgcggtctgt gagccccaca tggtggtccc aggaatggtt
     4381 gtcagaattg gcgactttgg gccagacaag ttcgacgaca cgggtgccgg cctctatgag
     4441 tcggcgggcc agcaagcagc tttggccgaa ggtattgcgt ccgtagcggt cacgcaattt
     4501 gacgggctcg cgctccaggg caaaggcgtc gcgcgcgcgt ccgctggcga tgaggctcaa
     4561 tgcctggtcg tagtagctgt cgaggctgag gtctttgaca gcggcctcaa tggttggcat
     4621 ctggtcgttg atggcgtcgc gcagcttggc gcggcgctgg aggcggacgc tgaagaggtc
     4681 gggacgcagc tgcagatcct cgatcttgat gcggtccatc ttgcccatgt ccatgtcatc
     4741 gccctcaggg tagagcgtgt agggatcata ggctttgccg aggaagccgg cggtgccgcc
     4801 tttgccgacg acgttggatt cctggagcgg gcggggcatc atgacgaagg gcagcatggg
     4861 ctcgtccatc ggcttgaggc ggatgatgtt gctgccgaag ttcgggtaat ccttcgcgtt
     4921 cggcggttcg agctggccgg aggggctgac cttgtcggtc gtgtagccgg tcatgatctg
     4981 gtagatggcc gccgtgtggt tgaacaagcc gttgggcgta tagctcatcg aattgatcat
     5041 ggtgaacttg tcgttcacct tcgccagctg gggcaggatc tccgtgaaat gatggcccgg
     5101 caccttggtg ggaatggttt tgaaggcgga gcgcaccttg tccggcacgt tctccttcgg
     5161 atcccaaaga tcgagatggc tgggaccgcc ctggaggtag atcatgagca catgcttcgc
     5221 cttgccccag cccgcgcggc ccgcagcggc ggagttcgcg gacgcggcct gggcgcggaa
     5281 catgccgttg agcgtcagcc ccatcatgga ggcgccgccc acgcgcagga tgtcgcggcg
     5341 ggtcatgccg aggtctttgt cgcagagatc tttgccgagg gcgccgggga tgcggaacat
     5401 ggaggtggga tgggttgagg attgcgcggt ttaaaatacg ggttcccgcc taacaaagac
     5461 aagaacaaac ttccacgctt atacgacccg ccgccggact tcttgccgcc gcgcgctcag
     5521 ggaagccgga aaatccattg ccagggctga tccagccagg gttgcggcgc atcgtggtca
     5581 ctttgttttt ccaggcaccg tcatgccggc gggtggcaga tcggccgggc caaatccagc
     5641 gttgtttttt cccgggggcg attcattttc gccttccccc aatgcttcgc acccttctgc
     5701 tctccaaaat ccaccgcgcc gcggtgaccg acgcctccgt ggactacgaa ggcagtctgg
     5761 ccatagacat cgaactcatg gacgcctgcg gcatggcgcc ctacgagcgc atcctggtct
     5821 cgaacatgag caacgccaag cgcttcgaga cctacgccat cgaggcgccg gcgggcagca
     5881 ggaccatcca gctcaacggc gccgccgcgc acctcggcaa acccggcgac cgcgtgacga
     5941 tcatgagttt cgcccaggtg acgccggagc agatggccgc gcaccagccg aaagtcatcg
     6001 tgctcaacga gcgcaacgag atcgtcaacc accgcggcat ctgaagtggc tccaacggga
     6061 gttccatcca tgccccagcc tccgggcggc atcgccgaaa aaccgcgtcc gattttttct
     6121 gaaaatcacc ggcggcatgg tatggcaggc gcatgagccg gccttccacg ctttcctccg
     6181 tcttcgagcg cgtttactcc ctggatgtcg tttttcagcc ggaatgctgg aagctgtgcg
     6241 gggacgcgca ttgctgcagc ttcgcccgcc acaaggagcg cttccgcctg cttggccgca
     6301 tggggccgcc cgcgcaggaa ctgccgctgc tgccgggcga gtatgacttt ctccacgccc
     6361 gcggctggac ggcgcagttt cacgggcatg agagaaagct cgcgccattc gacttcggcc
     6421 ccgcggtcat ccatctcgac acgatcacga gcacccgccc gcactgcgcc tgcgaccacg
     6481 acacccgcac caccgtctgc cgcctgtatc cgctgctgcc ggtgtttgac acggctgggg
     6541 ctctcgtgtc cacggagcct ctcggcatct acgaggagct ggaaatcctc gacgggctgg
     6601 cccccgcctg ccgccttgat tccctgccgt tttcccagct caatctttac ctggaactcg
     6661 cggcgctgct cggcgcgcat cccgttgcgc gttttcacct catggcctac cgcctggtga
     6721 aacggcatgt ggcggagcga gtgaggaatg aaaaaaacgc cggcggacgt gatgcgttcg
     6781 ccgtctttga agccgcctac atgcgccgca agctgctcga tcaagagaca ctgaaagccg
     6841 ctcttgctga gttgtgggag gcttttgtct cgcggcacgg cgcggatttc accgatgcgc
     6901 tggctgagtt gcgaaaggcg tgtccgcccg gggcatcctg acgaaaacaa ggctggtctg
     6961 aaaaaatgct ggagtctgga gtggcgcgcc gttttctgcg ttcctaatga tcgcacgatt
     7021 cctattcctt gcctgcctga cttttcagtt cgccgcccat ggcgaggacg ccgggcgctt
     7081 caaactcacc gcccgcgcca gcgaactgga cccgcgcgcc cgggagcacc cggagattga
     7141 ttttgtcttc tccaaagacg gcaaacccca ggatgtgcaa aacgcctcgg tggacccgcg
     7201 cgtgcccatg cggggaaaac tggtcgtctg gctcatgggc cacaacgacg cgctttttga
     7261 gaggctcaac agttatggtc tgcacgccat ccaggtgcat tacgccaaca aatggttcgg
     7321 cattctcaag cctgaagacc gcttggcgcg cggccgggtg cgtctggagg ccgccaccgg
     7381 gcgggatgtg agcgcccagc tccaaattcc ccagcccgac ggcatgatgg agcgcgcttt
     7441 ccaattcgtg aagtggctcg acaaggaaaa ccccgccgga aaatggggcc agttcatcag
     7501 cggggacggc gcgggcattc gctgggacaa ggtcatcatc agcggcagtt cccacggctc
     7561 caccacggcg gcgcgattcg ccaaggaagt agccgtggac cgcgtcgtca tgctctgcgg
     7621 tccgcgcgac caggagcagg actggcaggc cctgccctcc gccacggacc cgcgccgcta
     7681 cttcggcttc tcccatgtgc tcgacggcgg ctggaccggt gatcattact gccgctcatg
     7741 ggagctgctg ggcatgcatg agtttggccc gattgtcact gtggacaatg ccgcgccgcc
     7801 gtatgaaaac tcccgccgcc tcatttccgc cgccgatgtc gggggcgatg ccggcaaggc
     7861 ccacggcgcc gtcacacccg gcaaaagcag cccgaaggcg gacgatggca ccctgctcta
     7921 cgaagctgtc tggaaatacc tctacaccca ccccgtcgaa ctggtgggcc agcccggaca
     7981 acccgaccca gactgccaga aggagcatcc gctgccacgc tgaatccaga tatcccccgc
     8041 catgctttcc acgaacttcg ggaaacatgc acggcataga cgacctcccc aaaacagccg
     8101 cgtgaaacgg gaatgctcag cattcccaaa ccaagttttt tcaaaaaacc gcgctaaagt
     8161 cttgatttgt ccggccaaaa cctgaaaaaa gtgaacgctt accgctaaat tcggtaccaa
     8221 agccccccca tataccaacc cttatgttcc tcacagccct caaagccaaa ctcgccgcca
     8281 tggtcgttgt cgccggtctg aacaccgatg acatgcccgg catcagcaaa gacatgatgt
     8341 ccaccctgga aaacctcgcc gccgcccaaa aggccgccgc ggaagatccg ggcaagggcc
     8401 ctgacgtcaa cgcatccttc aacgaggccc tcgccaaggt caatgaactg gcgcagacgg
     8461 gcgacaagga cgcccagtac gctctggccc actggggcgt gctgagcaac agcaacctta
     8521 acgaggtggt cgtcctctat cggaaggctg ccgagcaagg ccagctcctt gccaaggtcg
     8581 aactcgccca ggtgctgctc caggctggcc agcagaacga ggccagtgtc aaggaagcgg
     8641 tgaaactcat ccaggaagcc gaggccggtg ataacaaagt tgcccgccgt cttctcgcca
     8701 acctccatct cggcggcgtc ggcggagtcg aggccagtgt cgagaaagcc aaggcgctcc
     8761 tcgaaaaagg cagtgccgca ggcgatggcg aggccaccct cggtctgagc cagctttaca
     8821 cccagggtgc caccggactt cccaaggatg acaagaaggc cctcgaaatg ctcatcaaag
     8881 ccagtgacca gggcaacccc gtcgccatgt ccacctatgc cgcgcgtctt ttcgacggcg
     8941 atcccgaagg cagttcccag ctcgtgaaga aggaccctga gaaagccaag aaaatgtttg
     9001 aagacgccgc cgccaaaggc ttcgccgccg ccaaccgcct gctgggcgcc atttatgaaa
     9061 acggcatggg cggcaccgcc aaagacctca agaaagccgt cgaatactac accaaggccg
     9121 ccaacggcaa cgacgcccag gctcttttcc gcctcggcaa ctacttcgaa gccggcctga
     9181 cccagggcga aggggagaaa gccgagattg tcatccagaa gaatgacaaa agcgccctcg
     9241 acctctaccg cctcgccgcc cagaacggac tgccggaagc cttcttcaat gtcggtgtct
     9301 attacgagac cggtactgtc gtggacaagg accccgagaa agcctataat ttcttcctcc
     9361 gcgccgccaa caacggactt gcccaggcgc agtttcgtct ggccggactt tatcaaaacg
     9421 gcaccggcgt ggcccaggat gtcgtcgccg ccaaagcctg gtaccagctc gccgccgatc
     9481 gtggaaacgt ggccgcccag atcgccctcg gccagatgta tgaggccggt gtcggcggcc
     9541 cggcaaacat tgaggccgcc tcccagcaat acaccaatgc cgccgaggcc ggcgcgccgc
     9601 tcgccatgct gcgccttgcc agccttgccg agcgcgggct gaacaccgcc aaaaatgttc
     9661 ctgacaaagc ccgcgcgctc gcctgggcgg acttggcggt ggacgcctcc agcaatgccg
     9721 aactggctgt caaataccgc gacgaactca agaagacctt gaagaccgac gagatcgccg
     9781 aagccaagaa aatctacgac acgctcaaga aatcctcctc cgaagcagcc cccaaaggcg
     9841 gcgaaagcaa gtccactcgc taagcggaag tttgtcgcaa aatcagcttg tcaaagcagg
     9901 tcgtgacgct aatctccggc tcccctgacc agggggagcc ggttttattt tccggggcat
     9961 tagctcagtt ggtagagcgt ctcaatggca ttgagaaggt cagcggttcg aatccgctat
    10021 gctccaccaa atcaggagcg gccagacggg ccttgttggg aaaccaggaa tgcaactacg
    10081 gctttgcctc cgcgccgcgc ctgcggcaca gtcgccagcc ccatccactc cccatcccgt
    10141 gagccactcc aaacccaccg agccaaagaa aaagaaatcc ctcctcggcc atctcaaggc
    10201 cgggctgaaa aaaatcatcg gacttgggaa gaaaaaaacg tccgccgcgg agccaaaaca
    10261 cgccgacggg cgccacccgg ccaagatgca tccccatgcg cgtccgccgc gcgaggacca
    10321 caccgacggt gacagccgcc ccccgcgtgg tccgcggcgc gagcgagagg gcggacggga
    10381 aagaaaaccc cgccgtgaaa gcgaaaaccg cccgccccgc gaggaacgcg gcgagcgcaa
    10441 acgtgaaggg cgcgggcctc gcggtggtcg cgagcgttcc gccgaaccca gggcccccat
    10501 ccgtaacttc gagcctctgc ccccgccgcc gccgccgccc gtgcccgaag ggccgtatgc
    10561 tccggcattt gagaatctcg gtctcgcgcc cgctgtgctc gccggcatcc gcgaaagcgg
    10621 ttacgagacc cccaccaaca tccagtccca ggccatcccg gtcgtgcttg agggccgcga
    10681 cgtcgtcggc gcctcgcaga caggcacggg caaaacagcc gccttcgccc tgcccactct
    10741 cagccgcctg ggcgctccgg gcaagttccg ctgcctcgtg ctcgagccca cgcgcgaact
    10801 cgcggcgcag gtcgtcgagc agtttgaaaa atacggcagg cacaccgggc agcgcgtgct
    10861 gctcgttcac ggcggcgtcg gttatgaaaa acagcgccgc ggtttgcagg agggcgtgga
    10921 catcgtcgtc gccactcccg ggcgtctgct agatttcatg caggatggca ccgcaaggct
    10981 ggacgatgtg gaggtgctca tcctcgacga ggtggaccgc atgcttgaca tgggtttcct
    11041 gcccgacgtc cgccgcatcg tcgagcgcac gccgaagtcg cggcagaccc tgtttttctc
    11101 cgccaccatg ccgccgcaaa tcaagaccct ggcggacttc gcgctcaaag atcccgtcag
    11161 catcgagatc ggcatccgct tctcccccgc cgagactgtc agccatttca tgtatcccgt
    11221 ggccggcgac cagcgcgagg agctgctgct cgccatcctc aagcagactc atttcaattc
    11281 gatgatgatc ttcacccgca ccaaaatgca ggccgaccag ctctacggag ccatccgcca
    11341 gaccggcgag tacaaagtcg ccgtcatgca cagcgacatc agccagaaag accgcgaaaa
    11401 ggcgctcaag ggcttccgtg acggagagtt tgaggtcatc gtcgccaccg acctcgccgc
    11461 gcgcggtctc gacatcagcg gcgtcaccca cgtcatcaac ttcatggtgc ccgaaaactc
    11521 cgaggactat gtgcaccgca tcggacgcac cgggcgcgcg caaaaggagg gggacgcctt
    11581 caccctcttc agcgccgagg agctgcccta tgtggccagc atcgagcggc tcatcaatca
    11641 aaaaatcccc cgccgcaagc tggggggctt cagctacaag tacaccaccg tcctcgacga
    11701 ggaggacaag gcgcgcgcca tcctgcacgg gcgcaaaaag aagcggcgtt gacccggccc
    11761 gccgcctgtg gctcatttcc tcatgaaccg caggcctgcc attctcgccc tttgcctcct
    11821 ctccgccgcc tggtgccgcg ccgaactggc gctggagacg cccgtcattg aggtccaccc
    11881 caagcccgag gatgagcagg tcaccgtcca cttcgccttc accaacaaag gctccaagcc
    11941 cgtgcatgtg ctgacgctcg acagctcctg ctcctgcctc agcgccgacc tcgacaagcc
    12001 cgtctatcaa cccggcgaaa aaggctcagg caaggccgtc ttcaaagtct catccttcac
    12061 cggcaggcac gagaagacca tccacgccaa aaccgacgac ccagcccagc ctgagtgggt
    12121 catccccttc atgctcatcg tcccggagat catcaagatc gagcccaaga ccctccaatg
    12181 gtggctggga gacgagccgc aggcaaaagt cacccgcgtc atcatgagcg gtgacgaacc
    12241 cctccgcatc accaacatca gctccacgcg cgagaacgtc gaattcagct tcaaagaagt
    12301 ccagccaggg cgcgagtacg aggtcaccgt gaagccgaaa aacacccagg aagtcatgct
    12361 cggcgccctg cgcatcgaaa ccgacagcaa gatcgccaaa taccagcgcc agctcgcctt
    12421 cttctccgtc tatcgcaaac ccgcaagcct ggacgcaacc ccgcccgcgc catgatccgc
    12481 gccgtcttcc aggccttcat tctcggcggc atgaccctcc tggcagccgc ggccactcac
    12541 ctcctgcacc cgcgcgcgcc cgcgctttac ctccaggaca gtcctctccg cgagcacgag
    12601 atcaccctcc agcagattga ggagcgctgg cagaataagg tcctctggct cgacgcaagg
    12661 cccgacgagc aataccagga ggaccacatc cctggcgccc tccagctcaa cgagcaaaaa
    12721 ttcgacgagc atctcctggc cctgctcgac accctccaaa ccacggacat gcctgtcatc
    12781 atctactgcg gaggagagaa atgcgaggcc agccaccgcg tccgcgaaaa gctcacccca
    12841 ctcgtgcctc tggacacctg cctcatcctc aaaggcggtt ggcccgcctg gaaagccgcc
    12901 cggcagtgaa ctccgcgcca gccctggcct gcaacaacct tgcacgcgcc agaatcccgg
    12961 cccataaaag cgcccatgcc cacacccgcg ctcccccacg actggctcgc gcaagccgcc
    13021 cgccaatggc cggacaccgt cttcctggag gatgagggca tttccctcac cttcgctgaa
    13081 agtctcgccc gtgtggaggc gctcgccggc tggatgcgcg cccagggtgt ggaaaaaggc
    13141 gcgcgcgtcc tggtctgcgc gccgaaccgc gtcgagctgc ccctcgtcgc cttcgccgcc
    13201 ctccgcctcg gcgcgctctt ttccctgctc tccccgcagc tccagcccgc cggtcttgcc
    13261 cgcatcctcg cgcaatgcga gcccgcgctc gtgctgctcg acgcgcccac cgcccacctg
    13321 cgcgagaccg ccgccgccag caccgcgcgc gtgcatttgc tggaggaaat ccccgccaca
    13381 ggcccagccg agccgcccgc cgcgctgaca cccgccgatg ccgccttcct cgtcttcacc
    13441 tccggcagca cgggcacccc gcgcggcgtc atcctcacac atggcaacgt cgccttcgta
    13501 gcccccgtca tccaggcgcg gctcggctat gaaaaaggcg actgcatcgg cgtcttcctg
    13561 cccctttcct tcgactacgc gctctaccag atgttttacg cctgcctcgc cggcgccacg
    13621 ctctccctgg cgcggccgga gatggccggg cccgagctgc ccggcgtgct cgcccggcgc
    13681 ggcatcaccg tcctgcccgg cgtgcccacc ctcttcgccg cactgctgaa aatgcagcgc
    13741 caccgcccgc gccccctgcc cgccctgcgc atgttcacca acacgggcga ccacctcccc
    13801 caggcgcaca tctcgcaact gcgcgagctt ttcccccacg cccgcgtctt ccccatgtac
    13861 ggactcaccg aatgcaagcg cgtctccatc ctcctgcccg aggagctgga cgcccaaccc
    13921 gcctccgtcg gccgcccgct ggatggcacc accgtcttcg ccgtggacgc cgaaggccgg
    13981 cgactgcccc ccggcgagct cggcgagctc gtcatccagg gcccccacct cagccccggc
    14041 tactggcgcg ccccggagga gacggccaaa cgcttccgcc acctggacgg cgtgcccacc
    14101 ctcttcagcg gcgaccaggg gcgcgtggac gaggccggct tcatcacctt ccactcgcgt
    14161 gatgacttcc tcatcaaaca ccgcggcacc cggctgaacc ccgccgaagt cgaagacgcc
    14221 gcctgcgccc tgcccggcat caccggcgcc ggctgcgtga aggacgagcc gcgcggtctg
    14281 ctccgcctct acctcaccac ccagggcgcg ccacccgccg ccgccgaagt gctcagcgcc
    14341 ctcgccaccg tgctcgagca ggccaagctg cccgaccgca tccaccccat ccccgagctg
    14401 ccccgcaccg ccaaccaaaa actcgaccgc aaagccctgc gcgccctggc cgaggcggag
    14461 gcgtgacaga atgcgaggat tttccggctt gtgaaatcaa catgggtgat ggatgaccgc
    14521 cttgccgcgt gagcggcgtc cgttttggcg aagccttccc gcggagcctg aggaacgaag
    14581 gcaaccacgc tttgacccgc acaccgactt gcgccgagtc tgggtgccca agggcgcgtg
    14641 cccggtggtg gagcggcaga ccaggcatga atgagactac ctttacggct cgctgaaggt
    14701 gaccggggga gaggcgcact ttgcccacct gcccaccgtg aacctcggct gtgacgcgct
    14761 ctatctcaaa gagctcgccg cgagcgatgc tgaggcagtg catgtcgtca tccgcgacaa
    14821 cgccgggttc catctgcgcg acggcgaccc acggctcccg gagcgggtgc gcatcgtggc
    14881 gctgccgccc cacagtccgg aacgcaaccg ctgcgagcat gcctgggaca tgatcaagga
    14941 tgaagtgtcc aatcagtgcc accgcatcac acaccacgcg cagcagctcc gcggcctcgc
    15001 gtgcgttcat cggcttgccg tggcactcat ctgccagcgt tttgcctccg atgaaatcca
    15061 tcgagtagta gtgctgtccc tcgtgcccgc caagctcgtg gatgctcacg atgttcgggt
    15121 gtttcaagtt tgccgccgcc tgcgcctccg cttgaaagcg ctgcacctcg tccgtgcccg
    15181 ccagcacgcc accgcggatc attttcaccg ccatcatgcg gctcagcttc acctgctgcg
    15241 ccttccacac cacgcccatg ccgccctcgt tgatttcttc gagcagttca taaccgcccg
    15301 cataccgcac gctcgatcct ttcgaccacg gcggcgcttc gagtttcacg gtgtcaaaat
    15361 tggccgatag cgccttgctc atcaggcagg ccgggcaaag cgcgtccggc gcgtcggagg
    15421 gaagtttttc gccgcataga ggacatgaat gggaggtgtt catggtggga tggaattggt
    15481 ttccatgggt tcctgaaagg tcggcggcga cgggttacag aaaaagttca ccatgtgccg
    15541 gcgaatttgc cgagatctca gggaagcatc gcgaggttgc cgctcgcaag gcccatgttg
    15601 catgcaggcg ccgaatcaca ctcgccaacg cagaagtgcc cgtcgcgcgc tgaatgatcc
    15661 gcactcgtag tgccacatgc gaaaggacgg aaaccgtttg gctttgctgt caatttcatc
    15721 atcaagctgc ttcacttctt ctccgctccg gccagcaggg ctttggcttc cttgcaggcg
    15781 agccagagga tgaggtcgtc gtggttggcg ccgtcagtca atgggttctg ctcatcggcg
    15841 gggaggggtt tcatcttcgc ttcggtggcg gtgaagaggg cgcgggcctc gccgggtttg
    15901 ccttggcgga acaggcacat cgaaaggtag aaactcgcgg tgacttcgat gcggggctgg
    15961 ttcgagctgt agttggtttc ggctgcggtt tggatggtgg tgccaagcgc aatggcggcc
    16021 tgcgggtagt ggccgccacg gtattcggcc atgccgaggg tcaggttcgc ccacggaagg
    16081 aatgtcttgg catcctggcc gagttccacc gctttccggg cgaggtccag gacggtttcc
    16141 tgggtcgcgg cgtcctcgat ggggcggagg gagacaagtt tggcgactcg ttcggcggtt
    16201 tcgtatgatt cggtgttggc cgccgattcc agcatccgct ggctggcggc ggcatgctcc
    16261 tggtctttcc cgaaccacac aagcagggat gcgtccttga gggccgaata aacgacgtca
    16321 ctcagggctt tggcggtttc gtccgctgaa ggcggcgctg tgggtgctgg catcgtcggt
    16381 tggccggggg cgggtgcgtt gctgccatcc agccgcttca ccttcacccc ggacaccgcc
    16441 cagccgccga agtgggcacc gagggcgagc gcgccgggct cggtggtggc ccaagcctcg
    16501 tgctggctga gggcggcggt ggggccggtc cattcgtaga gtggatgggc gtcgagagtg
    16561 gtagtgatgg tcgcgttcgc gccgtcgagg cgcacggtca cttgcaggtc gtgcggcttg
    16621 gagtcgttca cctgttggcc ctgcacggtt ccgggcaggt ctttgccgaa cttgccgttc
    16681 accaggatca ggccggtgta gatgccgccg tgaggacgcc catccagctc gaacccgcac
    16741 atccggtccg cgacggggag gacgacgtgg aaaacttgct tggtggcaag ctgccgcagc
    16801 ttcacgcgaa cggtgtagct gctcccggcg acgttgccgg gcagcggtag cgtcgcgtgt
    16861 ttcgcgttcg ggctgaaaag ctcgccgtct tccaggctcc acccgttgcc ggtctgctcg
    16921 acctcgttcg gggtgagcgg ggcgaggagg tcttcccagc cttcggcatc cttcgcaacc
    16981 ggcggcttgg tcacggcggg tcgtttcggc agcagggcgg cggcgctgga gcgctggcgg
    17041 tcccacacct tggtatcgcc gtcggtggcg atcgcggtga tgagcctgcc gtcggcgctg
    17101 atgtccgcat ccaccaccgg catggtgaga ccgtagaagg cgagccgttc ctcgccggtg
    17161 gcggcgtcgt ggaggcgcac ggtgccgagg ctgctgccac tgaggatctc gcgcccgtcg
    17221 gggctgaact tcacgcagcg cacgccctcc cagtgcgcgc ggaaggccgt ttccagcctg
    17281 cccgtggcag cctcccacac acgaacccag cggtcggggc cgccgctggc gagacgccgc
    17341 ccgtcggggc tggacgcgag gcaggcgatg ctgccggtct gcgctgcggc gatctggcgc
    17401 agcggtgtgc ccgcctgcca atcccacacg gtgatcccac cggtgggcgc ggcggtggcc
    17461 acggcggcat cgtcgaggca aaaagcggcg aagggctggc ggggattttc gaagggaatc
    17521 ctccgcagca ccttgcccgt ggggtattcc acgacgctga acgccgggcc gggataggtc
    17581 tgatgaaacg cgccttccac ctcgagaagg agccggccgt cgcggttgaa cttcacgctc
    17641 gaaccgtagt tgatgccaaa cggcgccagc gcttttccag tggacgacag gtggatgaac
    17701 tccttgatgt aaatcgcgga caaaacgacg gacccgtcaa cgggatggag agccagatcg
    17761 agagcccgat acttgtatcc cgcagaaaac gtccacttct cgacaccact ccggctgttc
    17821 caggcaacgg cattctgagc ggatgtgcag agggtcgagc cgtcacggtc gaacaatacc
    17881 cgcgagcaaa ttccgccctg acgcaccgcc gcgacctcct ccccgcggcg ggtcggccag
    17941 acatgcaaga tcgcgttttc cgcagccaca tgcgtggaca gcagcatcct cccgtcaggg
    18001 ctgaaagcga ggtcaacgac gtcggtgccc gctccattga acgaacgcag caaccgcccc
    18061 gtcgccgtgt cccacaggcg gatgcggctg ccgctgatgg tcgcgaccgc gtccccaccg
    18121 ggactgatca gcttcaccgt ctcgcccggg aaatggagtc cgcggatttt tgtggctgtc
    18181 tgcggattcc agaggatcac ctcgtcacgg ccgagacagg cgagccggtc acctgcctga
    18241 ttgaattgca gggcttcctt gccgtcattg tcggcactcg cttcgattgt gctgcgcacg
    18301 gtgccggtga gcgtgtcgcg gagttcgatc ttgagagcca gagccgtctt ggctgcgaca
    18361 atcgccacgg tccggccatc cgggctgaag gccggcacaa cgtagttgaa cggctgtgtc
    18421 cagagcggcg cgcccgtctg agccgcatag acgatgagtt gcttgccgcc ggcgaccagc
    18481 accgtcgctc catcggggct gagcagcacg tgcttgttct cgccgagttc acaggtccag
    18541 cgatgcacga gtttgccggt ggccacttcc tgcaccgcga cttcgctggc cgaggccgca
    18601 aatgcgatcc tgcctccggc gctgtcgatt cggaaggcac cagagccgtt caccggaacc
    18661 gagtcgccga tttgccggcc cgtgagggaa aagatgccga tgacaccgga ggaacacctg
    18721 gccgcgaaac gatctccctg cggcagaaac tgcaaccgca gcgcggcccc ctttccggga
    18781 agggaaaggc gggccgtgaa atcgctcgag tgagcctgca ggaaacgcca gttcgcatcg
    18841 cgaaagctct ccggtgtctc cgtgaggatc ttctgcgcct cgatgaattt gccgcgctcg
    18901 aattccttct ccgcgaggtc gatctgcgac ttcgccagcg cctgccgcgc cgtttccttc
    18961 tcctggaccg cgacggcttc ggcctccgtc gcgcgctgtt ccttggcctt caaattgaag
    19021 acgaaccaca cggcgagagc ggtgacgacg agccacgcgg caaaggcggt ggtgaagatg
    19081 cccttgttgc gcttgatcag cagcgccgcc tgcttggcga agcctgcgtt ctccgcgctt
    19141 gtcgcaaagc cggtctggta gcgctcgatg tcggcgctga acgcggcgac gttctgataa
    19201 cgcttcgcct tgtcgagcgc gagcgccttc atcgccaccg cagagagtgc ggcaggcacg
    19261 cgcccgcttt cgatgtgcgg cagcggcttg atctttttcg cctccagcac ctcgcccttc
    19321 acgtgtctgg catgggcgcc ggtggtgaca ccgaaggctg tgggcggcgt gatgcccccg
    19381 gtcttcacct tttccaaaat ctcatgcagg tccttcccgt ccaccggcgg ccgcagcgtg
    19441 aggatggcgt aaaggatgcc gccaagggag aagatgtccg agcgttcgtc gagttcggcg
    19501 atctgtccca tcgcctgctc tgggctcatg tatttcggcg tgcccatcac cgcgccctgg
    19561 agagtggcgg tcggggaggc accgctgggc gcggtgaatg acgaatcgga agccgccgac
    19621 tcacgcacct cgtcgttcag caccttcgca agtccccagt ccatcaccag cacctcgccg
    19681 aactcgccga ccatgatgtt ctccggcttc aagtcgcggt gcagcacgcc cttgctgtgg
    19741 gcgaaggcga cggcgtcgca gaccttgcgg aagatgagca gcagacggtc gagcgtgaag
    19801 tcgcgcaggt cctcggggtg ctcgttgcac agctcgttga ggatgtgctg gagcgtgcgg
    19861 cccttcacca gcttcatggt gtaaaacagc ggcaggccgt cctcccacac gatgtcgtaa
    19921 atgggaacga tgttgggatg cgaaagccgg gccagcacct cggcctcgcg gatgaagcgc
    19981 tgcttggtgc cttcatccat gtcgctgtcc agcagcacgg ccttcgccgc gacggtgcgg
    20041 gcgagtttgc ggtcttcggc ctccaggatg cagcccatgc cgccacgcgc gacctcgcgg
    20101 ccgagatggt agttcgcact tccctgcgcc ggttcaccgc ccgtcgcaaa tcgcactggg
    20161 ggagtcggcg gctgggtgcg gatggtttga aggtctgcgt cgggattcat ggtgttgctg
    20221 gttcccgacg aaatttttcc accgccgtaa caagctttta tttggcggcc tccgtatcag
    20281 cgagcgaagg cgggtcccag atgcgcgtgg tggcatcgag cgaggcgcag ccgagacgct
    20341 ggccgctggg ggcgaaggcg agggtgtgcg gcgcggcgag cgggccgcgc agttcgtcga
    20401 tccgcttacc ggtgtcgaga ttccagaggc ggatggtcag gtctgtcgag ccggtggcga
    20461 ggatgggttt cttcggatgc cacgccagcg cggtaatggg gccgtcatgg gcgcggaact
    20521 cattcaatac ctccagcgtg gcggcatcgc ggatgcgcac cttcttgtcc gcgcctgcct
    20581 cggcgatgcg gcgggcatct ggcgacacgg cgagcaggtc catctgaccg ggattcgttt
    20641 tcctgtaaag agcctcgcca gtggtgatgt cccagacgac gatttgttct tcggatcctt
    20701 tctcgccgcg cggagcatgg gcggtcacca agcccgccaa tcgctcatca ttcagccagc
    20761 cgacatcgcc aaaccggatg aaatttcccc cgttcagtcg tggcaaagac acgcctgtgg
    20821 cggtgtcctt cacctccgct ttttcaaagt tatgccacac gatcgccacc cgttttccgg
    20881 ttggacagag acggtggctt tcgcgccagg gcacgtcggg tatttcgctg gctttcacgt
    20941 tcatgccgtc tttgtgaagc aaaagaagcg ggtcatttct gcgctttgtc ccgacagtgg
    21001 cgaaccggcc gtcggcgctg agactgatgg agaaatagtc agcaagagag gcactccata
    21061 gcgaattggg agttgctgaa ccgaggcgct gcagagttgc agcctttctg ccatcgggga
    21121 agaagcccag atcgtcctcc ccccaaaacg ccacgccggc ctcgcgaacg ccgcgcaaaa
    21181 cccaccgtgc catccccaga ctccaggcgc gggtttcagg tccggtgacg accagttcgc
    21241 cggtgagcgg gtgcaggcat gcgccagtga catctccact tccgcccagc agtgactgca
    21301 caggagcgcc cgtcgtcgca tcccagatgc gcagcgcctg gcgtccgtcg tccagttttg
    21361 ctatggtgac gaggcggctg ccattggcgg tgaaaaggat gtgttcgatg ggccgattgt
    21421 ccgtgcgaaa ttcaaagacg atgtgctggt ctgtcggatc atatcctttg actgccccgc
    21481 gggcgtctcc cgtgacaagc acctgaccgt caggccggag gaccaatgcc caagtattca
    21541 acaggcgaac actccgcacc accgagccat cgtttaaact caacaggact gcccctccac
    21601 ggctctggac ccacaattgc tgaccaccgg gggcgaggtt cattgtcagg tttctccctt
    21661 cgccgactaa ttccgcgtat gtccactgaa gccgtcccga ttcgacatcc cagaacttaa
    21721 tggtccgcgc accttctgaa tccaccgcag cctgcgccag gctcttgcca tctggaccaa
    21781 aggccaaggc cacggtgcgc gccgcatccc attggcggag cggcttcccg ttccgggcat
    21841 catgaatcgt gattcccaca tggtctgatc tgacctgcca gcgcccaacg gcgatgcgct
    21901 ctccgtccgg cgagatcgcg atgcgatagg ctttgaaacg aatggcgggg aattcgcttc
    21961 cggtgctgaa ctccagcagg cgtttcccgg tgcgcacgtt cagcagggtc acgatgccat
    22021 catggtccac caccacgaac acgctgggca gcttcggatg cgcgacaacg tcgtcgatgt
    22081 cggtccccgt gtcgatccgg gcgatggagg aatccgactg cccgcgaagg tagtgccagg
    22141 tggagtcgcg caggtcttcg ggcacatcgc cgagcgcggc ctgcatcatc gaaccgttgc
    22201 cctcgcgcag cgcagcctcg gccagggaga gcgcggaacg ggcgagagcg ctgaccgtgg
    22261 cttgtttttc ctgaatggcg acggcttccg cctggctggc gcgcgcggcc tctgccaccg
    22321 ccgattgctc tccggcgatg gcgcgtttct cactggcgac gagccgcgcc acgaaaccga
    22381 tgctcaagag caccatcacc cccaggaggg cggtgaccgc cttgtgtctc agcatcagga
    22441 gaccgagctg cttcagcgtg ccggcgtgtt ccgccgtggt ggcgaaaccg ctctgatagc
    22501 gctcgatctc agcgctcaat gcagccacat cctgataacg cttcgtcttg tcgagcgtga
    22561 gcgctttcat cgccaccgcc gaaagggctg gcggcacgcg accggacggc aggtggggcg
    22621 gcggttgaag ttttttcgcc tccgccacgc cattcttctt tggtgcaacg gcttcacttt
    22681 tcgttgttcc ataggaagtg ggaggagtga tgctcccggt cttcactttt tcgagcagct
    22741 cttccacgga ctttcccgcc acgggagggc gaagcgtgag gatcgcgtat aggatggcac
    22801 cgagggaata gacatccgag cgctcgtcga gcaggttcgt ttcacccgcc gcttgctcag
    22861 gggacatgta ttgcggcgtg ccgagcagtg cgccggacag cgttccgctg aatgacgaat
    22921 cccgaattcc agatgacgaa tcgggggccg ctggttcagg cgctctggca ttcgtcattc
    22981 gacattcgtc atccagcatt ttggcgagtc cccagtccat caccagcacc tcgccgaact
    23041 cgcccaccat gatgttttcc ggcttcagat cgcgatgcag cacccccttg ctgtgggcga
    23101 aggcgatggc gtcgcagact ttgcgaaaga tcgcgagcag gcggtcgagc gtgtattccc
    23161 ggagcacccc ggagttttga tgcctcaggt ccttgaggat cgcctgcaga gtccggccct
    23221 tcaccagctt catggtgtaa aacaggggca cgccgccttc ccacacgatg tcgtgaatcg
    23281 gcacgatgtt gggatgagcg agctgggcca gcacctcggc ctcgcggatg aagcgctgct
    23341 tcgtggcctc gtccatgtcg ctgtcgagca tgaccgcctt cgccgccacc gtgcgggcga
    23401 gtttgcgatc ctcggcctcc aggatgcagc ccatgccgcc gcgcgcgacc tcgcggccga
    23461 gatggtagtt cgcgctgccc agcgccggct cgccacccgt ggtcaccggc accggacgaa
    23521 ccggcggccg gatgcgggtg gtgggagtgt ctgtgtcggg attcatggtg ccggtggttc
    23581 ccaacgaaat ttctgtgagg ccgtaacaaa ctcatttggc ggccagcacg gaccgcagcc
    23641 aggccagttc cccgggaatg tcctccggcg tcagcaccgt gcaggccagc tcctcctcca
    23701 gcagcgcgcg gaatttgctt ctcagacgca tgatctgcac tcgcgaggcc gcctcgctgc
    23761 agccgagctt ttgcgcgatc tcgcgatgcg acggcggctc ctcgtcccac atcagaaagg
    23821 ggagcagcgt ttcaaaaacc tccgcgcgtc cggcgacggc aaaggccgcg cgcagttttt
    23881 cgcgaatgcc ggccagcaga tcgttcgccc aggcgtgggt gaagagccat tcgggatcac
    23941 gcatgtcctg cggctcgcag gcgtagcgct cctccgcctg catttcgtcg atggacagga
    24001 gtttctggcc gccgccgcgc ttttgcgcgt tgtggtgacg cgcctcgtcg ctgagcaggt
    24061 gcttgagcga gccgagcagg aaggaacgca gtttcccacc gtcgagctgg atgatctgga
    24121 gcgtctcccg tttgatcagc cgcatgaaaa aggcctgcgt gaggtcctcc gcgtccggcg
    24181 cgctgtgacg gctgcggcgc aggtaggcgt agatcggata ccagtagcgc tggcagatct
    24241 ggctcatcgc cgcctccgcg tcggccgggt cgccgctctg cacggcccgc accagcgtcc
    24301 actgcgtggg cgggaaggaa ctgtcactgg catttggatc tggcgaggac atttcgggct
    24361 ggcgaatgac catttttaaa gtccggccag cctgcgggca ctcttttttt caggaatctt
    24421 caccgcagcc tgttacgccg gaaaagaaac cgggtcgggt aggggcagac gctccaaacc
    24481 catgaaacac tccgctccat ctcatctcac cgtctgcatt cttaccttcg tcatgctgca
    24541 ttcgatattc gtcattccgc ttccagcagc agtgccgcag ctcctcaact accagggccg
    24601 cgtcgccgtc ggcacggtga acttcgacgg caccggcgca ttcaagttcg cgctcgtgaa
    24661 cacggacggc accaccactt actggagcaa cgacggcacc agcaccgccg gctcgcagcc
    24721 gaccgccgcc gtcgcgctaa cggtgacgaa gggcctctac tccgtgcagc tcggcgatac
    24781 tacactggcc aacatgacca ccgtcccggc cagtgccttc gccaatgccg acgtgcgcct
    24841 gcgcgtgtgg ttcgacgacg gcgtgaacgg ctcccagctc ctcacgcccg accagcgcat
    24901 caccgccgtg ggctacgcca tgatggcagg cgaggcgcag acggtggcgg atggtgcgat
    24961 cacgggcgcg aagatcgccg ccggtgccgt gggcggctca caactcggag cgggcgcggt
    25021 gacgagtgcg aagatcgcag ttggagctgt cgggtctgga gcgctttctt caaatgcggt
    25081 gaacacaagc agcatcgccg atggcagcat cctcaacgcg gacatttcca attccgccgc
    25141 cattgacgcg acgaagatcg gctccggcag tgtgagcaac acgatgttcg gctatttgaa
    25201 cggagtcacc gcaccgatcc aatttcaaat cagcaatgtc ttgaacggcg ccacgagcgg
    25261 cacttacacc ggcagtttct cgggcgccca caccggtaac ggctccgctt tgaccagctt
    25321 gaatgggggc agtctccaac cgggctcggt cgggtcggac agcatcgccg acagcagcat
    25381 cgccaacgcg gacatttcca attccgccgc catcgctgac accaagctcg cgaccatcag
    25441 cacagcgggc aaagtggcca actccgccac caccgccacg acagcgggca cgcccaactc
    25501 catcgtcctg cgcagcgcct ctggaatcat cgtcggcaca atcgacggcg gcgggttgat
    25561 ctcgggcagt gtgagcggca ccagcatcgc caacggcagc atcaccgact ccaaactcgc
    25621 caccatcagt accgcgggca aagtggccaa ctccgccacc actgtcacga cagcggccac
    25681 gcccaactcc atcgtccagc gcgatggcgc cggattgatc cccggcacat tcaacggcag
    25741 cagcatcgtt tcgggctcca tcggttccag cgcgttaagt tcgagcacgg tgaataccaa
    25801 caacatcgcc gacggcagca tcttcaacgt ggacatcgct tccacggccg ccatcgatgc
    25861 cgcgaaaatc ggttcaggga gcgtgagcaa cacggagttc ggtttcctga acggagtaac
    25921 cgcgccgatc cagtcgcagt tcagcaacgt gctgagcggc gctcaaagcg gctctttcac
    25981 cggcagtttc gccggcacgc acaccggcaa tggttcgggc ctggccggaa tctccgccac
    26041 caacttcagc ggctcgctcg caggcgatgt caccggcaca cagggcgcta ccgtcgtcag
    26101 cgctgtggcc ggcgtcaccg ccgccgatgt cgctgccggg gcgaccctcg cgaatgaagc
    26161 aagagtcgca aacatcgcca acacgcttgt gaagcggagt gcgggcggga acttctccgc
    26221 cggcacgatc acggcggaga acctcaatgt gagcggcacg gtgacggcaa cggtgatcca
    26281 gggcgacggc tcgggtctga gcggtgtcac cgccagcgcg ctgtccagcg acggcgtcaa
    26341 tgccgtcctg tcagtgctag ccgcccgcgc gcaagcaaag gcggatgcgc ttacggcgat
    26401 cattccgacc ctcggcccgg cgatcactca aaagcaggcc gacatcaccg cattgaccac
    26461 ccagatcagc agtctcaatg cgcaaatcca gatgattgag gtccaacgtt ccgtggctca
    26521 aacaaactac gatgagcagc gggacgacgc gattttcgat tacacatggg gcaacccctt
    26581 gaatcagcct aatccgtccg accccacgac ctgggtgccc atccacaccc aagccgaaat
    26641 caatgccttc atcgctgcat acccgccctt cgatggatca tcgtttgatc agcaagttgc
    26701 aaacgtgaac gccaccaagg ctgccgctca ggcaacgaag acaactgcgg agcaggaatt
    26761 ggcggcactt caaacgcagt tggaaaatgt gaacattgac ctccgggcag cattgaccct
    26821 cgcagacatc gcgggcggac tctgaatcaa cccaaaacac cacaccgacc catgaagact
    26881 ctacttctct cactcgccac gctaatcccc gccgcagtcc acgccgaccc acgctcatcg
    26941 gccaactaca cccagcccgc agaagtgtca gacgccggcg gtcggcgcac gacgagcgcg
    27001 acctacgcga atgatggcag cgttggcttc gtgaccggca tctccatcgc tgccgctccg
    27061 gcgcagacgg cgaaggcggg ctacatcggc cagctctacg acgtgaccgg cctcgcactc
    27121 acaagcgacg cgccggaggt ggaggaaacc aagcggctcc agcttgccgc gtggcagacg
    27181 ctagacgatg cgaccttcct ctcggtcctc gcaacggacg tgacgtggag cgtgcagagc
    27241 ggaccgctcg tcaatgtgag cgcagccggg aaagcgaagg cggatgtcgt ctatgaagac
    27301 acctccgccg tggcgcaggc ggtttaagcc gagggcaccg ccacgatcgg tttcaccgtg
    27361 ctcgacacca tcgccgacaa cttcggcacc tacgcgggcg acgggctcgg cgacgactgg
    27421 caggtgcaat acttcggccc gaacaacccg aatgccgcgc ccggattcat cagcgacggc
    27481 agcgggctga cgaacctttt caaatacacc gccggcctcg cgccgaacga tgcggcatcc
    27541 acctttatcc tgaacaacac gcccgttacc aaccagcccg gcaagcagca gatcacactg
    27601 ggtccaacct tcagcgaccg gacctacacc atcgaattca gcctcgatct caaaaattgg
    27661 cacacgctcg gcccagcctt ccccggcaac ggcggcacac agatcatcac cgacaccaac
    27721 gcctccggcc cgcacaagtt ctaccgcgtg agcgtcaaca agccgtaatc agccggaggt
    27781 gcccggttca cgtcggactg cgtcgttcga tgcgtgggca tctgtttgat catcgcgtcc
    27841 gccggaaact cgcacgccat caggcatgcc tggcacagac tctccggcgt gtcagcggct
    27901 tgagctgtgc cttgcttccg gcattgatgt ggaggaatca tgacaggaat tcgttgtgcc
    27961 cgctccttct tggaagaaag cagggggcgg gctgcgaaaa agtcacgatg agcgcaaaac
    28021 agccaccaga tgtcgcattt cgtcgtccac atcaccgggc gaattgaccg tctcggcgat
    28081 ctccgcgcgc aaaacctcgc gataccgctg ccgcagccgg tgaaccgcca ctttgagcgc
    28141 gccctcgctc atgccgagct ttttcgccag ttcctcgcgc gatgtctccg cgccgctgag
    28201 cgtgcctttg agcgcgtcga aggttgattc gtcctttttg gccgcaaact cggctcgcaa
    28261 cttttccatg gcgcgagtca gctactccat ggcccagcgt ttgtcgaaga tgacatcagc
    28321 atcaatggga tccgccgact ccagagagta gcgcgcctcc ggctcgagcg cgtcccactc
    28381 cacgatctgc cgcccgccgc cgcgcttctg cgcattcgcc cgatgaaaat cgttcgcgag
    28441 gaagtgcttc agcgcaccga gcagatacga ccggaaccgt ccgcgcctcg gatcggccgc
    28501 cgtcgcattt gtctccatca gcgtggcaaa aaacgcctgc gtgagatcct gcgcgtcatg
    28561 cggcgcatgg ccacggcgac gcacaaaggc gtagagcgga caccagtagt cgcggcacag
    28621 ctccgacatc gccttcagcg cccgcgtgcc atccccgcca tgctgcgccg ccagcaccac
    28681 gctccatcgc gtggtgtgaa actcacgcgc gccgggtgcg atggagtcgt tcatagcgcc
    28741 ggcattcaaa tctacgcagg tggctttcgt caatctcctt ccttcccgcc gccgaacggc
    28801 gtggctcatc gaagcccgct cagagggtgc gctttcgccg catcccatgc cagctcttgc
    28861 agcaggcggt tgagagcctc cccgtgggct tccttgccca acgccgcagg acagggcaga
    28921 ccaatcggtg aacggcggta aatcacggcg aagtggcagt aggcagtgag ccattgcagc
    28981 ggcgcagtcg catggccgat ggcgtcggtg aacaggtcgt tctgctgttt gaggcccggt
    29041 gcctctcccg cgatgatctt cgcacgcaac gccagcgagg cctgccccac aggaacgacg
    29101 aaaacgacct gcttcccgta tttctcattc agagcacgca catggtcatc catgcttttg
    29161 tagtagggtt cgttgatcga gcgcagttcc gcgacggtgc gggagttccg gtccacaggc
    29221 tcgggcttct tctttttgta gttcacgtca aagacatcga acggcagcca gaactcctgg
    29281 atcgtgacgc ggatgtcagg ccggtgctcg aaacccagtt tggcaaaatt ttcgatcccg
    29341 tcgtccggca gataaatcgg cgagagcgtg agcacatcca cctggccggc gcgcagggcc
    29401 gttttgagcg tgttcttctc atccgccacg ttccaatgtt ggtaaacata ggagccgccg
    29461 atcgagctga tgcccgcctg ctggtgatcg ggtattttgg ccgccttggc gagttcagcc
    29521 agcatcttgg gcatgaacat gtggaagctg tgccccgctg agaaaacgcg ctggcctttg
    29581 tcgatgggtt ccgccgcgaa aatcggcgat cccatgccga tgagcagcaa agcggtgatg
    29641 aggcgtgtgc gcagaagcgt tttcatggtg cgttgtctct ctattgaacg gtgaacttgg
    29701 ttttcagctc cacgggcttc ttttcgccgg gttggtcatc caccggccag ttggtctggt
    29761 agttgttttg cgcctcgcgc ggcttttcga gctgtttgaa gaccagcggc ttcacgagcc
    29821 acgtcacggt gtagtcgcct gcgggcagtt tgccaaggtt cacggcggtc acttcatacc
    29881 aggtgaaggt cttgaagtaa ttcccctgcc agatggcctc gtgcatcgtg atggtgaacg
    29941 cgttgccgct gcgctcgatc ttctcgatcc acacacggtc gttgtcatcg agctggcgcg
    30001 tgcggaaaag cagccagttc tcgtccgcag cggtcacggc gggctttttc tcgcctgcct
    30061 cgtccatcca gtcgtcgagc ggcatgcctt gtggcttcgt ggtgtgcagc agcattgccg
    30121 cgtcgttcca tttgcccgtg tcgccgcgca cgagctgccg ttgatcactc agcgtgccgc
    30181 gttgggtgcg gtctttgacc gctttgagga cttcctgcgc ctgcagcagg ctggtgaaga
    30241 gaagaaaaaa gcacgcgagt ttcatgacgg gtcacttctt gttttgaaac cgatacagcc
    30301 acgaatacgt gcggatgaac aaactgccgc gtgcgatggc tggcgtggcc cacgcgacat
    30361 caccgagcga gtttttgcgg atgagtttgt attcggggcc gtcttcgatc acccacgtgt
    30421 cgccgttttc gttcagcatg aagacgcgac cgttgtaaga ccatggcgag gcataaaagc
    30481 gaccgccctt cgtgttcaac cgtgtcttcg gaatgatctc ctcgccggtt ttggcgttga
    30541 agcagccgag caggccggag tcgtagcaga cgaagagccg ctcgccgctg atgaggtagc
    30601 ccgggtgaat gctggagcca cgcttctgtg accacacgat gaattcgttg ctcgtcgcgt
    30661 cgccggtgag tgagatgtcg cccttcgcgc cgggtttgac cgcgtagagc gggccgtagt
    30721 gatagcccgc gcccgcgtag agcagtccgt gcttcgagag cgggatctgg ctcacgagtc
    30781 cggccgtgcc gctgatctcc cacagcaacg cgccgtcctc caccgcgtag gagcgaactt
    30841 tgttcaagcc aatcgtcacc agttcggtgc gctggccgct ctcccacgca tacggcgtgg
    30901 accaggtgct gcgttcctcg cgtgacacac gccacagcgt cttgcccgtc agcttgtcca
    30961 ccgtttcgag gaaggattcc tggtcgttgt cattctggat gaagatacgc ccgccatgca
    31021 gcacgggtga agtgccggtg ttccagccat cgcgcatcgg atagctcggc cagcggcgct
    31081 cccacagttt ttggccgttc atgtcgcagc aaaacaggcc gatgttcgca aagtaggcat
    31141 acacgcgctc gccatccgtc accggcgtct cgttcgcgaa cgaactgcgc ggatgccgca
    31201 tctgcggcgg gatgcctttg ttcgcctcga actcccacag catcttcccc gactcaaaat
    31261 cgaagcaata cagcatccag cgatgcacct cacccgtgcc gacgtggccg cccggatacc
    31321 cgcccaccgt cggcgtcggc aattccttct cgcccaccgc cgcggtgacg aaaaccttgt
    31381 caccccacac aatcggcgag gaccagcccc agcccggcac cttcaccttc cacaccacat
    31441 tctccgtgtc gctccacgtt tccggcagtg tcgcgccttc accgagccca tccgcggccg
    31501 ctccacgaaa ctggggccag tttggagtct ccgccgccca aacagatgcg gcgagggcaa
    31561 gaagaatgaa agctgctttc atgatgatgc ggttgctcgt caatacgtca gtcagccctc
    31621 ctctctgact tattcaccag ccgtcgagca cgaatgtgta tccttcaatg gtgcaggcgc
    31681 ttaaccctag ttgggccatt ggcgctgcaa actggcggac tgaggaatga ggacggcata
    31741 tcgcaattcc gacacgccat caggcaggtc ttcagccatg cgagggacgg cggaaaccga
    31801 tacaaatgag agcgaatccg ccgccaacgc ggcatcaaat ctgttgtcct ggactttaga
    31861 gaagtctgat ccaagcgcct caagcaactt ggcctccaca gcgaacgctg cgtccgcaga
    31921 tagttcggac tggatttcgc gcagcagttc aatgcagttg atgccaccgc tcgagcgttc
    31981 cacaaacacg gatgcgatcc aaagctcgat gccggatggc ggatgcaact gctcgtgtga
    32041 gaagtgatgt ctgcgaacac ggtttgcaga gcttttcact tctacacgaa gcctttgcat
    32101 agcgaagtcg tagcgttcca tgggatcagt gtgccagcca agggaccagc gggggacgtc
    32161 aggagagttg gcgagaacga aaagctcggc ccaaagacct tggatgctct tcaccgctgg
    32221 ttggcgaagc gcctgaaata tctgcaccag ttttcttacc tcgattgcaa cggcgcgcgg
    32281 ctcaggcaat tctgagaggc cgttcagcag atgaccagcg agacgaagaa aggattctat
    32341 cagcgcaaca tcactcaccc ggcactcgac aatgctaaat acccctgtca ccacgccatc
    32401 aggtgtattc atcgtgcctg cgtatccatg aaatacggtg aggttttcca gcttcaaaga
    32461 tgggggataa gctccgactg gaagacgcgt ctcaatcacg agaaccgggt ggccttgctg
    32521 ggtcttggcg atgtagttgc ggcttgagtc cgcaattctt aacgcaaagt agctgacagc
    32581 tgccgtgcca tcatggggag atggcgtgag agaagcgaag gctggaagaa tgtcggccat
    32641 ctgggacaaa attagctgtt gtcctgcaca agccagtcct gagccatgac cgctggaacc
    32701 cagactgcaa taactggcac gtcattcact aatggagcat cgttgtcagt gaggttgagc
    32761 ctgtgaagct ggatgctcaa ctgaccatct gcgtgaaggt ggcgctgacc tgaatcgtaa
    32821 ttctgatcac ctggattacc gtttgaaccc ataaacagct gtttgatttc gtcagtttgc
    32881 gaatcaatgc cacggctact ggggacataa ggactgattt gatagactgt gcagagttcg
    32941 ttggggtgcc cttgaaggtg ctgatgaatt tgcagcaatg cgccaaagta tttttgcccg
    33001 tcgtctggag ctctcacatg aacctgcgac agaagtcctt cataaacttc ttggagcggg
    33061 atgcccgcag ccatccgatg ctgtctagca tgggtgcgat gaacgttctc cggcaagtcg
    33121 acgaaggcat gacctgcaac aaacgagtcg aacaagttgc gattcgccgt cactgcgtcg
    33181 tccgattcat gaggcgaacg gacccagaac cattcgtccg catatgaatc tctcacatga
    33241 tcgaggctta acacattcct tcgagtcgga tggagattcc gcccgaggag gaatgcgcgc
    33301 ttccactcct gaaccggtcg ccctgtttgt ttccactcgt tcaactggag ccgcacgttt
    33361 tcttcgtgcg tcacatagtc agcgaacgca tcctgaacaa ccggatcgag gtaaacacgg
    33421 cagaagccga tgtagcgtcg cttgtagcca aagaatcgtg cgcgttgttg cagggtgtca
    33481 gcgtttccag ttccaagacc tcggggcatg taggtcacgg tcagaccctc gaccgtgaat
    33541 ccgcgatcaa gagcttgccc gccaaccaga atccatgcgt aaccagctcg ccaatccaca
    33601 tcagctcttc tcgaagttga gttcacttcg gaaatattgg tcttacggat ggcgcgcttt
    33661 aagactgccc ggacagcatc ccaaggggga agtgatgagc caactgttgt agctagttcg
    33721 gcgtaagcgg cttggaaagc atgaatctca tcagcgtagt ccggctctgt ttcaggaagc
    33781 tcaagcgcgc ttacccagcg atctttgatc agacggaccc agtgtgcaaa atcaccctgt
    33841 gggtcggttt cgcgggatgg atggatcaac atggaccgat tgcctctccc gccctcaact
    33901 gtgcccgcag caacaccgac gaaaaagaac cgcatggcat caattagact ctcgggcggt
    33961 gcaacgagag gagcctgcgg tgccacatca agaatgggat gaatgcggct tgctgcgccc
    34021 gagaagaact cgatcccgcc aacgtagtct gcaccgggat tcaacagctc ggcaaaatca
    34081 ggggagagcg tgtcgaggat gctgatcagc aacggcgctt gcggtgttgc tgtgtattgc
    34141 agaaacgagt gattgggaaa caagcgtcga agctgcaaga gccgagcata tgtagtgctt
    34201 tcttcctgct gcctgaccct cgtatttagg ctcgcttgat ctgcttcatc atcaatgatc
    34261 aacgctggaa tgcccgacct atccacgttg ttcaatagcg tgatgagttt gcgcaagacg
    34321 ctccagtttt tcttcactgt gatcagcaca aactggcgct cgtgtgcagg ggtatcatga
    34381 tctcgccaat cctcgaagat ttggttgaga cgttcgcgtt ccttgcggtc tggc
//
LOCUS       DAOMYJ010000030        32119 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Prosthecobacter sp. isolate AMR_MDS_5629
            NODE_508_length_32119_cov_21.773640, whole genome shotgun sequence.
ACCESSION   DAOMYJ010000030 DAOMYJ010000000
VERSION     DAOMYJ010000030.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867850
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prosthecobacter sp. (wastewater metagenome)
  ORGANISM  Prosthecobacter sp.
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Verrucomicrobiaceae; Prosthecobacter.
REFERENCE   1  (bases 1 to 32119)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 32119)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.30
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:34:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,742
            CDSs (total)                      :: 4,684
            Genes (coding)                    :: 4,676
            CDSs (with protein)               :: 4,676
            Genes (RNA)                       :: 58
            tRNAs                             :: 55
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32119
                     /organism="Prosthecobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_508_length_32119_cov_21.773640"
                     /isolate="AMR_MDS_5629"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1965333"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..429)
                     /locus_tag="PLP58_05170"
     CDS             complement(<1..429)
                     /locus_tag="PLP58_05170"
                     /inference="COORDINATES: protein motif:HMM:NF023324.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3416 domain-containing protein"
                     /protein_id="HRJ07937.1"
                     /translation="MSAAHAPTVVIENFYPCLEGGRHPVKRVPGEPLEVWCDIFTDGH
                     VVMSAQLKWRLQGTRRWFEAPMSHVDNDRWKGVCDFDAVGRWEYAVEAWADTFRGWKK
                     TFVVRVGADDPDVPVEALEGARLLREARVRPGIVMALIAGD"
     gene            complement(498..2471)
                     /locus_tag="PLP58_05175"
     CDS             complement(498..2471)
                     /locus_tag="PLP58_05175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009962257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aldolase/short-chain dehydrogenase"
                     /protein_id="HRJ07938.1"
                     /translation="MHSLWNDTEAATFGTDALGQRVYTSRLLGRNPELVLHGGGNTSV
                     KVTEKDYFGDSVDICYVKGSGWDLATIERAGFAPVRMEALLKMAKLPSMTDEDMVLQQ
                     RAAMTNPNAPAASIEAILHAILPFKFVDHSHANAISALTCAKDGEARTKELFGSKVLV
                     LPYVMPGFVLAKQVFEVLQKTDLRQEGIEGIVLLKHGLFTFADDAKASYEKHIELVTK
                     AENYLAQKSVNNVTPVNKVTPDLLTLAQLRQLVSKTRGFPQIARLSTSEAALSYGALP
                     NIADCATRGPLTPDHSIRTKRIPVVLGEDHAASVQKFADDYAAYFAKHASSQTMLDAA
                     PRFAVWPGNGIVSFGDTLKDAKIVADIAESTAATVALGERTGGWAPLSEKDIFDIEYW
                     DLEQAKLRKGPARKVHQGKVALVTGCAAGIGFAIAESLAEQGAQVVGLDIDKEIPEIM
                     AKIGAIGIVVNLTEDQPVQDAIDLTVREFGGIDIVVSNAGIFPKNDALDVMAQPDWDR
                     TLMINLTSHQKLMNKVIPYVKHSVDGSIIFVGSRNFKAPGPGASAYSCSKAALTQLCR
                     VAALELAPWKIRVNIVHPDAVFDTKLWTPEALAKSAARYNMTVEEYKTKNLMKVEIRS
                     KDIGNMVAAMASPLFAKVTGAQIPVDGGNDRVI"
     gene            2624..3655
                     /locus_tag="PLP58_05180"
     CDS             2624..3655
                     /locus_tag="PLP58_05180"
                     /inference="COORDINATES: protein motif:HMM:NF012692.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HRJ07939.1"
                     /translation="MPAKPPPKSKKPAKARKPKVKKTPFWVGFDLGGTKMLACVLDAG
                     YNVLGTARKTTNGSDGQIKGRKKIVNAIHEAIAEAGVDPAGLQGIGIGCPGLVNPERG
                     ILINAPNLGWNNMGLAGILKNAFNKPVAVLNDVDAGTFGEYKLGAGKGARSLLGVFPG
                     TGVGAGFVYDGRLIMGRTVSAMELGMIYLPGTHLGSAEFGAVILEDLTSRLALASQAG
                     VACYRGQLPELDRKTRGNLRDLRSKALANSYKAGDEAALVMFRNGVRYLGMGVAAVVN
                     LFAPDHITLGGGLVEELPDLYLRTLKEEVARYALPPLARGVRYSLAKLGGGAVAVGSV
                     AWLRENSGK"
     gene            3662..5206
                     /locus_tag="PLP58_05185"
     CDS             3662..5206
                     /locus_tag="PLP58_05185"
                     /inference="COORDINATES: protein motif:HMM:NF014587.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HRJ07940.1"
                     /translation="MLLPDTPPSREHAILHVGAASISLIIGTRLETGGFSLLEHLDRP
                     LALARDIFRGGVISRSTVEQAAGILKDYQSSLREYGVSAAHTRLHATNILAEAVNHEI
                     FLNRLQVTTGLAARLMDDGDMTRFIYQVSQRLFQANPALREGHTFVTHIGPGNTRALY
                     FKAGRLAAYSNYRLGIFRAREAVAVEEGDAPRQLSHLEEHIRGVVDHLAADYSGYTTE
                     HHVAIGAEIQSVAAEMTAAKNGLRCILERDLERVAGKLALLSPDEMVRKLHVHYTGAE
                     GVVPALQTNLALARRFGDERLWVPEGDFQRELMLDLATAGPRLENFQDEVIQAACEIG
                     VRYKTDRKHADHVAAFAQQLFRELQHLHGLDAKYELVLRVAALLHEVGMFISPREHHK
                     HSLYILLQTEIFGLSSGDRVLVALLARYHRRYNPEPNHPHFADLSREERMIVFKLAAL
                     LRIADALDRTHAQRVKSILIRPDGGRLHILTPGVDDTTVEQIAINSKCGIFSEIYGYE
                     VVLSRP"
     gene            complement(5252..6136)
                     /locus_tag="PLP58_05190"
     CDS             complement(5252..6136)
                     /locus_tag="PLP58_05190"
                     /inference="COORDINATES: protein motif:HMM:NF012647.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prenyltransferase/squalene oxidase
                     repeat-containing protein"
                     /protein_id="HRJ07941.1"
                     /translation="MSFRLEMLQVARVAPKLLGESAALVADFLKSRLHESGGFLDRAD
                     RPDLYYTVFGLEGLMALQANMPAGKTRNWLGCFGDGEGLDFVHLCCLARCHASLGMTA
                     FPEVARERLAARIERWRAPDGGYHQAAGRGNGSAYGCLIAWGAYQDLGLLPPDALAIA
                     GCMDRLETPDGAWSNEPGLTFGSTTATAAMLSLYRHLQMPPPPAALAWLERQCLPDGG
                     FLAVPGAPVPDLLSTAVALHALSDQPEIIARVKEPCLNFLDSLWSAAGGFHGNWTDDV
                     LDPEYTYYGLLALGHLAL"
     gene            complement(6139..6993)
                     /locus_tag="PLP58_05195"
     CDS             complement(6139..6993)
                     /locus_tag="PLP58_05195"
                     /EC_number="3.1.1.99"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009963210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMP-30/gluconolactonase/LRE family protein"
                     /protein_id="HRJ07942.1"
                     /translation="MPITPEPAGAHVSLWGEGPIWHQDRLLYVDIEAHKILAFDPVSS
                     AEKIWNAGQRVGTVVPRVKGGLVWAGDTGFHFLDEATGFSTPIADPEPDLPDNRFNDG
                     KCDPAGRLWAGSICLKRRPDAALYCLHTDLRVEKKFSPVTNSNGIIWSHDGGTMYYID
                     TPSKKLRAFDFDTATSAISGERVIWDTSDDPSSPDGMTIDSEGRLWVAFCHGAKVVCF
                     DPASRAVLEQIDFPCVETTACAFGGPDLADLYVTTGIKPGLEEPLGGRLFVCRPGARG
                     VPSTAFAG"
     gene            7141..8565
                     /locus_tag="PLP58_05200"
     CDS             7141..8565
                     /locus_tag="PLP58_05200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009961456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase"
                     /protein_id="HRJ07943.1"
                     /translation="MRTLFFSAVLSLAFAGLSIHAAESPNLVVIYMDDMGYADVGCFG
                     AQGYATPNIDRLAEGGRKFTNFHVAQAVCSASRAALLTGCYPNRIGIHGALGPKSTHG
                     IHADEMTLAEVVKQKGYATAAVGKWHLGHLPQFLPTRHGFDEYYGLPYSNDMWPYHPE
                     ARPGTYPPLPMMENEKVVDAEVTPEDQTRLTTDYTTRAVRFIERSKDKPFFLYMAHSM
                     THVPLFVSGKFAGKSGKGLYADVMMEVDWSVGQVMDTLEKHGLTEKTWIIFTSDNGPW
                     LSYGDHAGSAGPLREGKGTAWEGGTRVSCVMRWPGQIAAGTTSDAMMATMDVLPTVAG
                     VIGAELPKHKIDGMDLWQVVSGGPEAKNPHDFYAWYYQNNQLQAVSSGDGRWKLVLPH
                     GFRTLNGRPGGMGGTPVKYEQSRVQEPELYDLYADISESKNVAAQNPEVVSKLQSHAE
                     QMRADLGDMLMKKATGTGTREAGK"
     gene            8632..12480
                     /gene="hrpA"
                     /locus_tag="PLP58_05205"
     CDS             8632..12480
                     /gene="hrpA"
                     /locus_tag="PLP58_05205"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009963455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase HrpA"
                     /protein_id="HRJ07944.1"
                     /translation="MIQYPADLPITPRREDILAALGAHQVVILAGETGSGKTTQIPKM
                     CLELLALQQGAQHGPQASRPGFPPGLIGCTQPRRVAAMSVSRRVAEELGVAWGREVGC
                     KMRFSDDTSRETRVKFMTDGILLAEIQGDPLLRAYSMLILDEAHERSLNIDFLLGYLK
                     QLLPRRPDLKLVVTSATIDTEAFSAHFGGAPIIEVSGRLYPVEVRYRPVGDEDDDPSH
                     IEEAVSAVTEALKESAEGDVLVFMPTERDIRETRDLLEGRLAPACEVMALFGRMASQD
                     QQRVFSPGPKRRVIVATNVAETSITLPRIRYVVDTGLARMSRYNPRTRTKRLPVEAVS
                     QSSANQRAGRAGRVQDGVCIRLYSEEDFQKRPRFTQPEIQRANLAEVILRMKAFRLGQ
                     IEDFPFINPPSSASVRGGYDLLHELGALNDTHEMTGLGRELARLPLDPTLGRMLLQAR
                     EEGCLDDMLILAAGLSIPDPRERPEEKKELAQAAHKTFASPESDFLSLLKIWRAAPPP
                     QDGARNALRRFCKAHFLSFTRMVEWRDLWRELGRSGILPDRRGSGILPDSGETGILPV
                     SESTGQNARSTRDRQDACPTDRLHRCILAGHLGHIAQRVGRNIYKAAGNREVTLFPGS
                     HLYERKDKHDKSGQEKTRQPLWIVAAEIVQTSQLFARTAAGINPEWAAEFGAHLVAKK
                     YSEPHWSAKAGRVLVTERHLLHGLEVMRRKIDFGKIDPVAATQLFIRGALLEEGASLP
                     HRFFQHNQKIRSRLEAALTRVRSSRVYAIEEHLFEFYRARIHGISSVHDLNKLVNERV
                     KTEPDFLCVREEDLTRGDDIRDGLDQFPDKAPLGNSVLPITYAYKPGQDEDGVTVRVP
                     LPVAEHLTSGQLQWLVPGLREELVLTLLRALPKATRRALMPLEAAAREIAREFDPGRA
                     DFLTAFAAFLRERHRLEVQAADWPADSLPAHLRPRVEVVGAQNKPVIATRDLGEIRAL
                     ARQQERRSDAWEKAAARLEKHAIRAWSFGDVPEQVLIEEIGGAPVHGYPGLQRTEEGV
                     SLRLFRRAEEAQRHSPAAVRQLAEEALGKDIAWLRKELRALDQPAPKSTGLASLAMLD
                     LSAKAAPTSPAELSAQACEHILAHALRLEPLLPLKVARFREMCERARREFPTLAHRVR
                     DLLKQCEDLKKKILAFPRPYTGLKDDLARLMPPDMLTRTPHAQLQHLPRYLKAILLRA
                     ERASNHPAKDADKAALIRDFDGWERECGEANREAFRWLFEEYRVSVFAQELGTAQPAS
                     VKRLEALLGAESLEYRQCSA"
     gene            12573..14927
                     /locus_tag="PLP58_05210"
     CDS             12573..14927
                     /locus_tag="PLP58_05210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007416732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HRJ07945.1"
                     /translation="MKKPRFILAALAASLLSSHAAQQVWVEAESFADHGGWMLDTQFI
                     DIMGSPYLLAHGMGTPVRDARTTVAVTEAGSYKVWVRTKNWVGPWEAPGAPGKFQVGV
                     NGVKLEKVLGTEGRDWLWELAGEARLSAGQAELSLHDLTGFEGRVDAVLLTQDAGFTP
                     SADFAATNALRRQSLGLPQEAPETEEYDLVVVGGGYSGMGAAISGARQGLKVAFIQNR
                     PVLGGNGSSEIQVWAMGGTRRGLYPHLGEIIEEFADRASNSPAASPEEFNDKLKEQVV
                     RAEANIDLFLNTHVHGITMEKGDTLKIRSVTGLDTRTGKETRFRGKFFTDCTGHGSVG
                     ALAGAEILMEEKGRMGMSNMWVLKKTANPKPWPATPWALPLEQGDFPEPRVLEPAGVK
                     NKANMTGYDLGYTPVENAEDYLHGEWFWESGFDQDPINGLERIRDWNFRAVYGAVSAM
                     KRYAPDKFGNYDMTWLAYVGGTRESRRIVGDFILKGEDMVKGVIQNDACVPTTWDQDL
                     HYPKEQYSVKFPENPFISRAEFGKHTDRKNGYPVPYRCFYSKDVSNLFMAGRCISVDR
                     WALGSTRVMRTCGMMGEVVGKAAWISVRHHTTPRGVYEQYLDILKDLMSKPGAMRRDS
                     LEGALYLPANAKKLPEVVSDSIDPRKLEGIVIDDADAELTGPWATGEGLKPFVADHYS
                     YSQAKEASARFSFAVKETGKYEVFIYWQPHANRAKAAPVSVLSAGGEKTFRVNQSQPA
                     AGKQGAHSLGTFTFNAGEESAVTFRTEGSQGNVHLDAVQVVRVP"
     gene            15023..16081
                     /locus_tag="PLP58_05215"
     CDS             15023..16081
                     /locus_tag="PLP58_05215"
                     /inference="COORDINATES: protein motif:HMM:NF013567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HRJ07946.1"
                     /translation="MHSSTRRRLFDPPLVSAAASVLSRIFLFTLFAVSAAFAQNDLKP
                     LRVGIVGLDTSHVPAFTKLFNKGETEGELAGIKVTTGYTGGTDMPASANRKEKFTQQL
                     RDMGVEIVPTIPELLAKVDVVLLESVDGRIHLQEAREIFKAGKPVFIDKPLAGTLAEA
                     IAIVELAKKHNVPFFSSSSSRFGAEMVALKANTDLGDILGAQTWGPCSYQEGTPDFFF
                     YGVHGVEALYTLMGPGCETVSRVKAGNHDVATGVWKDGRVGAYRGIVKGKADFGAVAY
                     GSKAIGQAAKAVSYEGLCRQIGKFFRTGQPPVSAEETIEIFTFMEAADESMRQGGKPV
                     ALADVLAKAKAEAAKLIK"
     gene            16086..17129
                     /locus_tag="PLP58_05220"
     CDS             16086..17129
                     /locus_tag="PLP58_05220"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="sialidase family protein"
                     /protein_id="HRJ07947.1"
                     /translation="MRTLLFLFIGLLGALAAEPLQVSVSNIRRVFHNGEHNAFTDLVR
                     FNGALYLCLRSCPDGHMVFNTASVIVLRSTDQGATWQPVHRFSVKDRDTRDPHFLVFK
                     GRLFVYSGTWWTGPGLLDRKDYDMNKQLGYAVSSADGSKWSEPTLMEGTFGYYIWRAA
                     SHDGKVYLCGRRKANFAVEVMGERGHNQSIMLESDDGIIFRHRAYFQESGGNETAFFF
                     EKDGSVLALDRNGGGNSMLCSSKPPYTEWNRYPLDRFVGGPLLVKWGEHVLIGGRHQT
                     KDSGPKTSLGWLVGDKFHEFAELPSGGDNSYPGFVELSPTKGLISWYSSHEKDEAGKT
                     ITAIYLAELVISR"
     gene            complement(17139..17705)
                     /locus_tag="PLP58_05225"
     CDS             complement(17139..17705)
                     /locus_tag="PLP58_05225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ07948.1"
                     /translation="MDDTLRAALAAATELEKLGVRWFLGGSLASSIHGIPRATFDADI
                     MADLRPQHVRPFLRALGEAWYADEVAMQDAIRDRSSFNLIHLETAMKVDVFVPKLRRF
                     DGGEFARSQSLKLEGADIEARVCCAEDIVIAKLEWYRLGGGDSERQWGDILGVLRLNA
                     GKLDLELLQNSAAELGVADLLGKALAAR"
     gene            complement(17698..17940)
                     /locus_tag="PLP58_05230"
     CDS             complement(17698..17940)
                     /locus_tag="PLP58_05230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ07949.1"
                     /translation="MNDTSSTAEAMRLRALQAMTPTRRLGLALGWSQSVRELTRSSLH
                     QQHPELPPQELHRLLAERLLGEELAHKAYGPFISHG"
     gene            complement(18103..19437)
                     /locus_tag="PLP58_05235"
     CDS             complement(18103..19437)
                     /locus_tag="PLP58_05235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ07950.1"
                     /translation="MKRLALFICLSLKSFLLGEEHKGPVAEAYREIDLKIPEANQLLA
                     EGKWTEAVRLLNSVHPKDQRTVDQSQALASFMFQLHPEESYELHKEVAAAHPDLEMPN
                     YLWAVEQHRRGEWKGALASYRVASRLMKDYAPTYGLAAECALRLGKVDEALELWEKSE
                     KAQTGTLEEFETEVCKIHGPQPKHREREALMTRAKAGDFDAAVGVIELDLEWPEDWWN
                     SGPNKNFLQVDLATFAGLKEPGRELELALLAAKLVTEDLEPKKAKQILSDAGLLLRKP
                     VLPANGRVLSFLITYIHDHEFLPKETLTKNWGAVMRDQAKKSKDPELHNALAWLHLDS
                     PELESIDLQDWKQTGDARFAASYLSGQMEAEKLTLHSPQLIQALKQFPDHYVVQSLPL
                     GLTKRESPRYRSALIETIKADYVKLPRAGVIPRPSAKPLMQAFHELAEISKE"
     gene            19549..20985
                     /locus_tag="PLP58_05240"
     CDS             19549..20985
                     /locus_tag="PLP58_05240"
                     /inference="COORDINATES: protein motif:HMM:NF013567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HRJ07951.1"
                     /translation="MLTPTRRRFFEDSLMAAAAVALPKTLLGAETTTVSANEKITAAI
                     IGCGIRGKAHARELARLPECDVAYVCDPDLDRADEVGALLVELKRPMPKKVQDLRKVL
                     EDKAVDVVFIATPNHWHALAAIWAMQAGKDVYVEKPVSQNVEEGRRIVQVARKLGRIA
                     QTGTQNRSRGALAEAVKFMREGKLGEVKLAKSIIYSGRGSIGGPAECVMPPRCDYDLW
                     AGPAPMTKLTRPKFHYDWHWFWNTGNGEIGNNNVHSLDICRWGLGVIGLGRSVLSYGG
                     RLGYTDVAETPNSQVGIFDFGDKTIVSETRGLNTAPFHPTIKSMWFFYGSEGIIADTS
                     LFDPQGKLVRAFEGKSENHFANFIKAVRSRSHTDLTADIEEGHQSTALCHIANISYRL
                     GSKTSVNDISKALGAIQVHEDVQHTLERTRHYLAEAGVDLDKTQLTLGQHLRVDGDKE
                     SFLDNAAANALLTREYRAPFVVPNLSAI"
     gene            21016..22968
                     /locus_tag="PLP58_05245"
     CDS             21016..22968
                     /locus_tag="PLP58_05245"
                     /inference="COORDINATES: protein motif:HMM:NF038032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CehA/McbA family metallohydrolase"
                     /protein_id="HRJ07952.1"
                     /translation="MLRLFWILGSFAFACSLGAQDITLEARRLHLGKAGEWEWEIFKD
                     VPVDAGKMELRFQANVNTSEHTLRIWQRDVKQSWPVMLNGRRLGVLTLAETALECVLA
                     VPPGALRDGKNVLLIDSPARLDDIEIGPMHLTLKPVAEAIGGAALLIEVKDGTHGEAM
                     PCRLTLTRPDGTLQPLQAAPAGDVAVRVGVIYAKNGKARVSVPPGDYILHAGRGFEWG
                     VASRKLTMKQGETLPVDLTLSREVDTKGWIAADSHIHTLTHSGHGDAKIEERMITIAG
                     EGIELAIATDHNHHTDYAPSAASVGVTDRFTSVIGNEVTTKHGHFNAFPIQPGAAVAN
                     HNEQDWAKLLPAIRATPGVKVITLNHPRDLHSGFVPLGGLQFNPTTGKHRHADALDID
                     ALEVITSGAMQSDIHLLYRDWFALLNRGHRIAAIASSDTHDVNRFILGQGRTYVAAKD
                     GNPSQLDLDEVWRSYQEGRLLVSLGLMTTLKVNDRFTVGDLATGLGSQIKVEAVVSGP
                     AWVTADRIELYANGILIREQSIKDEHRAGEKARITWELPKPAHDVHLVAIATGPGITE
                     PFWEIPRPYQPSSKSLTPRVIGSTNPIWLDCDGDGKFASAFAIAQALVTKLGSDSAAL
                     REALKKHDAAVIVQVESLLPNSIGKP"
     gene            23013..23777
                     /locus_tag="PLP58_05250"
     CDS             23013..23777
                     /locus_tag="PLP58_05250"
                     /inference="COORDINATES: protein motif:HMM:NF015297.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase/citrate lyase family protein"
                     /protein_id="HRJ07953.1"
                     /translation="MQPSVVLQKIRSGQTVITAKSCYTDPELVEMIASSGFDAVWICL
                     EHKRVDAAMVYALIQACRLGGADAFIRVRSSNYTDVLQLIEAGARGIMLPRVKHPAEA
                     REVVRAMKFPPLGARGFDGIHAEADFGRIQPAEYLAKANDENFLAVQIEEPEVVPHID
                     EIAAIPGVDVLFVGPADLTLGLGKFGKTDDHEVRAILEQVVAACQRHGKVAAIPCAPE
                     QVKSYHAMGFRFFNVISDFRCVSTGMKAARAAVELS"
     gene            23878..25752
                     /locus_tag="PLP58_05255"
     CDS             23878..25752
                     /locus_tag="PLP58_05255"
                     /inference="COORDINATES: protein motif:HMM:NF024240.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HRJ07954.1"
                     /translation="MPVTIHTLETDLLVAGGGIAGVCCALAAARLGARVILCQDRSVL
                     GGNASSEVRMHIVGATGLTGGEELQNELREGGIIEELRLDLAVQNPQRSPALMDLLLY
                     DKCRREPNLMLYLNTTVVSAVVEDGIIREVRAERPSTEDAFIIRAKTFVDCTGDGRLG
                     IEAGAPFMRGRESKTQFGESLAQDETDSKTLGSSIMFQARKHERAMLFEAPPWARRFS
                     AEDFKLRPYGQSGFDLGLEYGYWWIEWGGCLDTLKDNESIRDELLAITLGVWDFVKNH
                     SGLDASHWALEWIGFLPGKRESRRFIGQHILTENDLITSRPFPDAIAYGGWPIDTHPP
                     EGVDAPHEPPCTQHHLPFIYDIPLRCCVSSGPRNLMFAGRNLSATHIAFASTRVMATC
                     GVVGQGVGTAAALALRQNTLPADIADDEELVTSIQQRLLRDDCYLIGISNDDAHDLVR
                     SAVHIRELDACGLVRQNEDSLPHSGECAYSPENIRSGITRAVTKVPPHRQSPGLHRWM
                     SRELPATVEIRWSQPVMLREVVLIFDTGLHRLLTLSQADGYTQKMLWGRPQPETIRDY
                     RLSVETTDGWRDVAVIQGNHQRRRAHLLEPCAVTALRVSISATNGMDHARVVEVRAYG
                     "
     gene            complement(25765..26334)
                     /locus_tag="PLP58_05260"
     CDS             complement(25765..26334)
                     /locus_tag="PLP58_05260"
                     /inference="COORDINATES: protein motif:HMM:NF012257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030551 - cyclic nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HRJ07955.1"
                     /translation="MKEYAYIHEEGQLPAALSAVPFLNSFTEEQLDEVLNSSSLLQCD
                     EGDVIIREGTIDSRIYILLSGELEVRVGDKKVASISRSGDVFGELALVNRDKRAASVI
                     ASNRALCLAVDQKFLQDIHPREEDPAFHAALFEFVARLVAKKLDATSRRLAEVEKELR
                     LLKEEAGKASAAPGRVRKFQPRRLRRVPI"
     gene            complement(26414..27037)
                     /gene="upp"
                     /locus_tag="PLP58_05265"
     CDS             complement(26414..27037)
                     /gene="upp"
                     /locus_tag="PLP58_05265"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: protein motif:HMM:NF001097.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004845 - uracil phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HRJ07956.1"
                     /translation="MNPPVILTHPLALSHLTEMRRFDTPPRRFRWHLHRLAEMLFLEA
                     SRSLDTSALRVLTPLAETAGAALARPIVLVPVLRAGLGLSEAILPLVPDAVVAHLGIR
                     RDEETAKPVPYYANLPAALAEADVFLLDPMLATGGSACAAAAQLKAAGARRMRMINVV
                     SCPEGLAALGAEHPDVPVFTAALDEGLNEKCYIVPGLGDAGDRCFGT"
     gene            complement(27952..30150)
                     /locus_tag="PLP58_05270"
     CDS             complement(27952..30150)
                     /locus_tag="PLP58_05270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009966102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HRJ07957.1"
                     /translation="MSSFQLNPDFHPRGDQAQAIAKLVKSLRAGNRHQTLLGVTGSGK
                     TFTMANVIAEMGRPALVFSHNKTLAAQLYSEFKNFFPANAVEYFVSYFDYYQPEAYIP
                     RTDTYIEKDSSINDEIERLRLSTMGALLTRRDVIVVASVSCIYGLGSPEDYEGMMVPI
                     HTGQQMSRETFLVKLVDMLYERNDVQLKRGSFRARGDVVEVVPAYLDSEAIRIEFFGD
                     EIDRISAVDILTGHVTLKMPSYTIFPAKQFVTPGDKLRKAIVKIREEMEQRVAFFEQE
                     GKLLEAQRLKMRTEHDIEMMQEMGFCQGIENYSRHLTGRQPGATPGTLLDFFDEPPLV
                     FVDESHATIPQIGGMYEGDRSRKTVLVEHGFRLPSALDNRPLKFPEFMEKAGQLVYVS
                     ATPARFEMENSVVGNTTYIPHARERIGQEETTPAALPGRSIRVSGNAQPVDKFDVATR
                     GKSLVVEQIIRPTGLLDPVITVKPLQGQIDETIELCRQRIERGERVLVTTLTKRTAED
                     LTDYLRNLDLKVRYLHSDIDAIERVEILRSLRKGDCDVLIGINLLREGLDLPEVSLVC
                     ILDADKEGFLRSETSLIQTAGRAARHVAGEVVLFADLQTQSIQALLSISAHRRQVQME
                     HNEKHGITPQTVRRAVQESLQILGKARETEENIVREGGGSDLAVTEVIRELEEEMAEA
                     AAKLEFERAALLRDQIRELKKQSGLSPDDGGVPPKPKKVTYGRPKRGKKK"
     gene            30422..31168
                     /locus_tag="PLP58_05275"
     CDS             30422..31168
                     /locus_tag="PLP58_05275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ07958.1"
                     /translation="MTFPTTHWTLLAEATLNGDAAGRQALSRMCEEYRKPVLAFLSAR
                     GYQEGEREDLAQEFFLNWLSTRSWKRAAREKGRFRSYLLGSVCHMLARHHERKHAAKR
                     GAGAGCDSLEAAAEAGWEAADETPPESPAFDRAWAVTLVMNTLHCLEQDFARRGRAGE
                     FAVLRRFLPSGGEGITLEEAARCLDFSTSAVKSAVHRLREAFRLALRAAVAGTVSAPH
                     EVDEELAYLRSLLLAGVESRNLSNEKRNMS"
     gene            31187..>32119
                     /locus_tag="PLP58_05280"
     CDS             31187..>32119
                     /locus_tag="PLP58_05280"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HRJ07959.1"
                     /translation="MTCPQCGAAIDPAEAGGMCVACLLGEAMQPRVCQDTLGVIGGHD
                     LLEELARGGMGIVYRARQAESGKEVALKALPGAELLSEEARRRFHIEALAMARLEHPC
                     ILPVHDTGEDDGTPWFTMKLAKGGTLAGRITEYSGKWRETAELIARIAEAVQFAHERG
                     VLHRDLKPGNILFDADDQPRVADFGLAKLLDEEMDLTRTIALMGTPNYMAPELTRGGK
                     GAATTACDVWSLGVMLYELLAGQPPFHGDNLATVLRQLNEEEPALLPRAVPCDLAVIT
                     RKALQKTPAQRYASAGALAADLRRWLRGEAIEARP"
BASE COUNT         5933 a         9856 c        10417 g         5913 t
ORIGIN      
        1 gtcgccggcg atcagcgcca tcacgattcc cgggcgtacc cgggcctcgc gcagcaggcg
       61 cgcgccttcc agcgcctcca cgggcacgtc gggatcatcc gcgccgacgc gcaccacgaa
      121 ggttttcttc cacccgcgga aggtgtccgc ccacgcctcc acggcatact cccagcggcc
      181 aacagcgtca aagtcgcaga cgcccttcca gcgatcgttg tccacatggc tcatcggcgc
      241 ctcgaaccag cggcgcgtgc cctggagccg ccacttgagc tgcgcgctca tcaccacatg
      301 gccgtcggtg aaaatgtcgc accacacctc caagggttcg cctgggaccc gcttgacggg
      361 atgacgcccg ccttccaggc agggataaaa attctcgatg acgactgtgg gtgcgtgtgc
      421 ggcggacatg ggagcgaaaa cgccacattg agccgctgcc gcgtggcgag gcaagttcgg
      481 aaattcgaca accttcctca aatcacccgg tcattcccgc catccaccgg aatctgcgca
      541 ccggtgacct tcgcaaacaa cggtgacgcc atggcggcga ccatgttgcc gatgtctttg
      601 gagcggatct cgaccttcat gaggttcttg gtcttgtatt cctcgacggt catgttgtag
      661 cgggcggcgc tcttggcgag ggcttcgggg gtccacagct tggtgtcgaa gacggcatcg
      721 gggtgcacga tgttgacgcg gatcttccac ggggcgagct cgagggcggc gacgcggcag
      781 agctgcgtga gcgcggcctt ggagcaggag taggcactcg cgccggggcc gggggctttg
      841 aagttgcggc tgccgacgaa gatgatgctg ccatcgacgc tgtgcttcac atacgggatg
      901 accttgttca tcagcttttg atggctggtg aggttgatca tgagggtgcg gtcccaatca
      961 ggctgcgcca tgacgtcgag cgcatcgttt ttcgggaaga tgccggcgtt gctgacgacg
     1021 atgtcgatgc cgccgaactc gcgcacggtt aagtcgatgg cgtcctgcac gggctggtcc
     1081 tcggtgaggt tcacgacgat gccgatggca ccaatcttcg ccatgatctc ggggatctcc
     1141 ttgtcgatgt cgagaccaac gacttgtgcg ccctgctcgg cgagcgattc ggcgatggcg
     1201 aagccgatgc cggcggcgca accggtgact agggcgacct tgccctgatg caccttgcgg
     1261 gcggggcctt tgcggagctt ggcctgctcg aggtcccagt attcgatgtc gaagatgtct
     1321 ttttcgctga gcggtgccca gccgccggtg cgctcgccga gcgcgacggt ggcggcggtg
     1381 ctttcggcga tgtcggcgac gattttggcg tccttcaagg tatcgccaaa gctgacgatg
     1441 ccattgccgg gccacacggc gaagcgcggg gcggcatcga gcatggtctg gctgctggcg
     1501 tgcttcgcga agtaggcggc gtagtcatcg gcgaacttct gcacgctggc ggcgtggtcc
     1561 tcaccgagca cgacggggat gcgcttcgtg cggatgctgt gatcgggggt caaaggcccg
     1621 cgcgtggcgc aatcggcgat gttgggcaat gcaccatagc tcaaagcggc ctcgctggtg
     1681 ctgaggcggg cgatttgcgg gaagccgcgg gtcttcgaga cgagctggcg gagctgggcg
     1741 agggtgagga ggtcgggggt gactttattg accggagtga cgttgttgac ggatttttgg
     1801 gcgaggtagt tttcggcctt ggtgacgagc tcgatgtgct tctcgtagct ggccttggcg
     1861 tcgtcggcga aggtgaagag gccgtgtttg agcaggacga tgccttcgat gccttcttgg
     1921 cgcagatcgg tcttctggag cacttcgaag acctgtttgg ccaaaacgaa gccgggcatc
     1981 acatagggca gcacgagcac tttggagccg aaaagctcct tcgtgcgggc ttcgccgtct
     2041 ttggcgcagg tgagggcgga aatggcgttg gcgtggctgt gatcaacgaa tttgaaaggc
     2101 aggatggcgt gcaggatggc ctcaatgctg gcagcgggtg cgttggggtt ggtcatcgcg
     2161 gcgcgttgct gcaggaccat gtcttcgtcg gtcatggagg gcagtttggc catcttcagc
     2221 agggcctcca tgcgcacggg ggcgaagccg gcccgttcga tcgtggcgag atcccagccg
     2281 ctgcccttca catagcaaat gtccacggaa tcgccgaagt agtccttctc ggtgactttg
     2341 acggaggtgt tgccaccgcc atgaagaacg agttccgggt tccggccgag caggcggctg
     2401 gtatagacgc gctgtccaag ggcgtcggtt ccgaaagtgg cggcttcagt gtcgttccag
     2461 aggctgtgca tggcgggaaa ggggagcgcc agacttccga tggatgggcc gtgaagcaag
     2521 gggattttgg ttcaggcggc gatgatcgaa tgcaggcggc ggattgttgc ttgccccggc
     2581 gcggaagccc gtcctcaata gcctcaatca tccaccaaca gccatgcctg cgaaaccgcc
     2641 gcccaaatcg aagaagcccg caaaagcacg gaagccgaaa gtgaaaaaga cgcccttctg
     2701 ggttggcttt gacctcggcg gcaccaagat gctggcctgc gttctcgacg ccggctacaa
     2761 cgtccttgga accgcgcgca agacgaccaa cggctccgac ggccagatca aaggcaggaa
     2821 aaaaatcgtc aacgccatcc atgaagcaat cgccgaggcg ggggtggacc cggcggggtt
     2881 gcaggggatt ggcattggct gtcccggtct ggtgaatcct gaaaggggca tcctgatcaa
     2941 cgctccgaac ctgggttgga acaacatggg cctggccggc attttaaaga acgctttcaa
     3001 caagcccgtg gcggtcctca atgatgtgga tgcgggaact ttcggcgaat acaagctcgg
     3061 cgcgggcaag ggcgcgcgct ccctgctcgg ggtttttccc ggcacgggcg tgggcgccgg
     3121 gttcgtctat gacggacgcc tcatcatggg acgcaccgtc tccgcgatgg agttgggcat
     3181 gatttatctg ccaggcacgc atttgggcag cgcggagttt ggcgcggtga ttctggagga
     3241 cctgaccagc aggcttgccc tggcctcgca ggcgggtgtg gcctgctatc gcgggcagct
     3301 cccggagctg gacagaaaaa cgcggggcaa tctgcgcgat ctgcgcagca aggctctggc
     3361 gaactcctac aaggcgggtg atgaggccgc gcttgtgatg ttccgcaacg gggtgcgtta
     3421 tctcggcatg ggtgtggccg ctgtggtgaa tcttttcgcg ccggaccata tcaccctcgg
     3481 cggcggtctg gtggaggagc ttccggattt gtatttgagg acactcaagg aggaggtggc
     3541 gcgttacgca ctgccgccgc tggcgcgcgg agtgcgctat tcgctggcaa agctgggcgg
     3601 cggcgcggtg gcggttggca gcgtggcctg gctgcgcgag aattcaggca agtgacacac
     3661 catgcttttg cctgacaccc cgccatcccg cgagcacgcc atccttcatg tcggcgccgc
     3721 ctccatctcg ctcatcatag gcacccggct tgagaccggc ggtttcagcc tgctggagca
     3781 tctggaccgc cccctggcgc tggcacgcga catttttcgc ggaggcgtca tctcccgttc
     3841 cacagtggag caggcggcgg gcattctgaa ggactaccag tccagcctgc gcgaatacgg
     3901 ggtgagcgcc gcgcacacgc gcctgcacgc caccaacatc ctggccgagg cggtgaacca
     3961 cgagattttt ctcaaccgcc tccaggtcac gaccggcctt gccgcgcgtc tgatggacga
     4021 tggcgacatg acccgcttca tttaccaggt cagccagcgt cttttccagg caaacccggc
     4081 tttgagggaa gggcacactt ttgtcaccca catcggtcct ggcaacaccc gggccttgta
     4141 tttcaaagcc ggacgtctgg ccgcttacag caactaccgc ctcggcatct tccgcgcccg
     4201 cgaggcggtg gctgtcgagg agggggacgc gccgcgccag ctcagccatc tggaagagca
     4261 tattcgcggt gtcgtggacc acctggccgc ggactattcc ggctatacca ccgagcatca
     4321 tgtggccatc ggcgctgaga tccagagtgt ggcagcggag atgactgcgg cgaaaaacgg
     4381 cctgcgctgc atcctggaga gagacctgga gcgggtggcg ggaaagctgg cgctgctgag
     4441 tcccgacgag atggtgcgca agctgcatgt gcattacacc ggcgcggaag gggtggtgcc
     4501 ggcgttgcag acaaatctgg ccctggcgcg caggttcggc gacgagcggc tctgggtgcc
     4561 ggagggggat ttccagcgcg agctcatgct cgatctcgcg acagcgggcc cgcgcctgga
     4621 aaacttccag gacgaggtca tccaggccgc ttgcgagatc ggtgtgcgct acaagacgga
     4681 ccgcaaacac gccgaccatg tggcggcctt tgcccagcag ttgttccgcg agctgcagca
     4741 cctccacggg ctggacgcca agtacgagct tgtgctccgg gtggccgccc tcctgcacga
     4801 ggtcggcatg ttcatcagcc cgcgcgagca tcacaagcac agcctctaca ttttgcttca
     4861 gacggaaatc ttcggcctca gcagcggcga ccgcgtgctg gtggccctgc tggcgcgcta
     4921 ccatcgccgc tacaaccccg agccgaacca tccgcacttc gccgatctct cgcgcgagga
     4981 gcgcatgatc gttttcaagc tggccgcgct tttgcgcatc gccgacgcgc tggaccgcac
     5041 ccatgcccag cgcgtcaaga gcatcctcat ccggcctgac ggcggccgcc tgcacatcct
     5101 caccccaggt gtggatgaca ccaccgtcga gcagatcgcc atcaactcga agtgcggcat
     5161 tttcagcgag atctacggtt acgaggtcgt gctgtcgaga ccgtaaagcc tcccggcctg
     5221 ggggcaaaac acgggcgtcg cacatgcgcg ttcacagcgc cagatgtcca agcgccagca
     5281 gtccatagta ggtgtattcc gggtcgagca cgtcatcggt ccagttgcca tggaaaccgc
     5341 ccgccgcgct ccagagtgag tcgagaaaat tcaggcacgg ttccttgact cgggcgataa
     5401 tttccggctg gtctgacaag gcatggagcg ccaccgccgt ggaaagcagg tctggcaccg
     5461 gcgcccccgg cacagccagg aagccgccat ccggcaggca ttgccgctcc agccaggcga
     5521 gcgccgcagg tggcggcggc atttgcaaat ggcggtacag cgaaagcatc gccgccgtgg
     5581 cggtggtgga gccgaatgtg agaccaggct cgttcgacca tgctccatcc ggcgtctcca
     5641 gccggtccat gcagcccgcg atggcgaggg cgtcaggcgg cagcaggccg aggtcctgat
     5701 aggcgcccca ggcgatgagg cagccgtagg cgctgccgtt gccgcgtcct gccgcctgat
     5761 gatagccgcc atcgggggcg cgccagcgtt cgatgcgcgc agccaggcgc tcgcgcgcga
     5821 cttctggaaa agcggtcatg cccaggctgg catggcagcg cgccaggcag caaaggtgga
     5881 caaaatcgag gccctcgcca tcgccgaagc agcccagcca attccgcgtt ttcccggcgg
     5941 gcatgtttgc ctgcaaagcc atcagcccct ccagtccgaa gacggtgtag tagagatcgg
     6001 gccggtccgc gcggtcgagg aaaccgccgc tctcatgcag gcgcgacttt aaaaaatccg
     6061 cgaccagcgc ggcggactcg cccagcagct tcggcgcgac gcgcgccact tgcagcattt
     6121 cgagacggaa cgacatcttc agcctgcgaa cgcggtggag ggcacgccgc gcgcgccggg
     6181 gcggcagaca aagagacgcc cgccgagcgg ctcttcgagt cccggcttga tgcctgtggt
     6241 cacataaaga tccgcgaggt ccggcccgcc aaaggcgcat gcggtggtct cgacgcaggg
     6301 aaaatcaatt tgctccagca cggcccgcga ggcggggtcg aagcacacca ccttcgcgcc
     6361 gtggcagaag gcgacccata ggcggccttc ggagtctatc gtcatgccat ccgggctgct
     6421 cggatcgtcc gaagtgtccc agatgacacg ctctccgctg atggcggagg tggcggtgtc
     6481 gaagtcaaag gcgcgcagct ttttgctcgg cgtgtctata tagtacattg tgccgccgtc
     6541 gtggctccag atgatgccgt tcgagtttgt gaccgggctg aatttctttt ccacgcgaag
     6601 gtccgtgtgc aggcagtaga gcgcggcatc ggggcgtctt ttcaagcaga tgctgcccgc
     6661 ccagagacgg cccgcggggt cgcacttgcc gtcgttgaag cggttgtctg gcaggtccgg
     6721 ctcaggatcg gcgatgggcg tgctgaagcc ggtggcttcg tcgagaaagt ggaatcccgt
     6781 gtcgcccgcc cagacgagac cgcccttcac gcgcggaacc acggtgccca cgcgctggcc
     6841 ggcattccag attttttccg cgctgctgac gggatcgaag gccagaattt tgtgcgcttc
     6901 gatgtccaca taaagcaggc ggtcctgatg ccagatgggg ccttcgcccc agagggagac
     6961 gtgggcgccg gcgggttcgg gtgtgatggg catggcgggt gggaagcgga atactgttcc
     7021 tggatgtaaa ggtggagccg ggggaggtca accgctgcga gcattgcgga atgggccaag
     7081 cgtcccgcat cttccaatcc gcaagaaagg cattcccttc gcccgcgttg actcatgccc
     7141 atgcgcacgc tttttttttc cgccgtcctg tcgctggctt tcgccggact ttccatccat
     7201 gccgctgaat caccgaacct ggtcgtcatt tacatggatg acatgggcta tgccgacgtg
     7261 ggctgcttcg gcgcccaggg ctatgccacg ccgaacattg accgccttgc ggagggcggt
     7321 agaaaattca ccaacttcca tgtggcgcag gccgtctgca gcgcatcgcg cgcggccctg
     7381 ctcaccggct gctacccgaa ccgcatcggc atccacggcg cgctcggacc gaagtccacc
     7441 cacggcatcc acgccgatga gatgacactg gccgaggtgg tgaagcaaaa aggctacgcg
     7501 accgcagccg tgggcaagtg gcatctcggc catctgccgc agttcctgcc cacgcggcac
     7561 ggctttgatg aatactacgg actgccctat tccaatgaca tgtggcctta ccatcccgag
     7621 gccaggccgg gcacctaccc gccgctgccc atgatggaaa atgaaaaagt cgtggatgcc
     7681 gaagtcacac ccgaggacca gacccgcctg accacggact acaccacgcg cgctgtcaga
     7741 ttcatcgagc gcagcaagga caagcccttc tttctttata tggcgcatag catgacgcat
     7801 gtgccgctgt ttgtgagcgg gaaattcgcc ggcaagtctg gcaaaggtct ctatgccgat
     7861 gtgatgatgg aggtggactg gtccgtgggg caggtgatgg acactctgga aaagcacggc
     7921 ctcacggaaa agacctggat cattttcacc tcggacaatg gcccctggct cagctatggc
     7981 gaccatgctg gctccgcagg cccgctgcgc gagggcaagg gcaccgcgtg ggagggcggc
     8041 acgcgcgtga gctgcgtgat gcgctggccg ggccagattg ccgccgggac cacgagtgat
     8101 gcgatgatgg ccacgatgga tgtgctgccg actgtggccg gcgtcatcgg cgcggagctg
     8161 ccgaagcaca agattgatgg catggatctc tggcaggtcg tcagtggcgg gcctgaggcg
     8221 aaaaatccgc acgacttcta tgcctggtat tatcaaaaca accagctcca ggctgtgagc
     8281 agcggtgatg gacgctggaa gctcgttctg ccgcacggct tccgcacctt gaatggccgc
     8341 cccggtggca tggggggcac tccagtgaag tatgagcaga gccgcgtgca ggagccggag
     8401 ctgtatgatc tttatgctga catcagcgag tcaaaaaacg tcgccgcgca gaatcccgag
     8461 gtcgtttcca agttgcaaag ccacgctgag cagatgcgcg cggacctggg tgatatgctg
     8521 atgaaaaaag ccaccggcac tggaacgcgg gaagcgggca agtaagctca gcttttctga
     8581 gcggagtcca gccttgcacg cgcagtcatc ttcctcaatt ctgcgcgcct tttgatccaa
     8641 taccccgcag acctccccat caccccgcgc cgcgaggaca tcctcgccgc gcttggagcg
     8701 caccaggtcg tgatccttgc cggtgagacg ggatcgggga aaaccacgca gatcccgaag
     8761 atgtgtttgg agctgctggc cttacagcaa ggcgcgcagc atgggccaca ggcgtcccgc
     8821 ccgggcttcc ctccgggcct catcggctgc acccagccgc gccgcgtggc cgccatgagc
     8881 gtctcccgcc gcgtggcgga ggagctgggc gtggcatggg gcagggaagt gggctgcaag
     8941 atgcgcttca gtgatgacac gagccgcgag acgcgcgtga agtttatgac ggacggcatc
     9001 ctgctcgccg agatccaggg cgatcctctg ctgcgcgcct actcgatgct catcctcgat
     9061 gaggcgcatg agcgctcgct gaacattgac ttcctgctcg gctacctgaa gcagcttttg
     9121 ccccgacggc ccgacctcaa gctcgtggtc acctccgcca ccatagacac cgaggccttc
     9181 agcgcccact tcggcggcgc gcccattatt gaggtctcgg gcaggcttta ccctgtggag
     9241 gtgcgctacc gtccagtcgg ggacgaggat gacgacccct cgcacattga ggaggcggtg
     9301 agcgcggtga cagaggcttt gaaagaatcc gccgaggggg atgtgctcgt cttcatgccc
     9361 acggagcgtg acatccgcga gacgcgggac ctgctggagg gccgcctcgc cccggcgtgc
     9421 gaggtgatgg cgctctttgg ccgcatggcc tcgcaggacc agcagcgcgt cttttcaccg
     9481 gggccaaagc ggcgcgtcat cgtggcgacg aacgtggccg agacctccat caccctgccg
     9541 cgcatccgtt atgtggtgga caccggcctg gcgcgcatga gccgctacaa tccccgcacc
     9601 cgcacgaagc gcctgcccgt ggaggccgtc agccagagca gcgccaatca acgcgcaggc
     9661 cgcgccgggc gcgtgcagga tggcgtgtgc atccgccttt acagcgagga ggacttccaa
     9721 aaacgcccgc gcttcaccca accggaaatc cagcgcgcga acctggccga ggtgatcctg
     9781 cgcatgaaag ccttccggtt aggccagatc gaggacttcc ccttcatcaa cccgccctcc
     9841 agcgcctccg tgcgcggcgg ctatgatctg ctgcacgagc ttggcgcgct gaatgacaca
     9901 catgagatga ccggcctggg ccgcgagctg gcgcggctgc cgctggaccc cacgctcggg
     9961 cgcatgctgc tccaggcgcg ggaggagggc tgcctggatg acatgctcat cctcgccgcc
    10021 ggtctcagca tccccgaccc gcgcgagcgg cccgaggaga aaaaagagct ggcccaggcc
    10081 gcgcacaaga ccttcgccag cccggagtcg gacttccttt ccctgctgaa aatctggcgc
    10141 gccgcgccgc cgccgcagga tggcgcgcgc aatgccctgc ggcgcttctg caaggcgcac
    10201 ttcctctcct tcacgcgcat ggtggagtgg cgggatcttt ggcgggagct gggcaggtcc
    10261 ggcattttgc cagacaggcg tgggtcaggc attctgcctg actcgggtga aacgggcatt
    10321 ttgcccgttt ctgaaagcac cgggcagaat gcccggtcca cacgcgacag gcaggatgcc
    10381 tgtcctaccg accgcctgca ccgctgcatc ctcgcggggc atctcggcca catcgcccag
    10441 cgtgtggggc ggaacattta caaggcggcg ggcaaccgcg aggtcacgct tttccccggc
    10501 tcgcacctct atgagcggaa ggacaagcac gacaaatccg gccaggaaaa aacccgacag
    10561 ccgctgtgga tcgtcgccgc cgagatcgtg cagacctcgc agctcttcgc gcgcacggcg
    10621 gcgggcatca accccgagtg ggcggcggag tttggcgcgc atttggtggc gaagaaatac
    10681 agcgagccgc actggagcgc caaagccggc cgcgtgctcg tcactgagcg gcacctgctg
    10741 cacgggttgg aggtcatgcg gcggaagata gacttcggca agatagaccc cgtggccgcc
    10801 acgcagctct tcattcgcgg cgcgctgctg gaggagggag ccagcctgcc gcaccgcttc
    10861 ttccagcata accaaaagat tcgcagccgc ctggaggccg cgctcacccg cgtgcgcagc
    10921 agccgcgtct atgccatcga ggagcacctc ttcgagttct accgcgcgcg cattcacggc
    10981 atctcctccg tgcatgacct caacaagctc gtgaacgagc gcgtcaaaac cgagcccgat
    11041 ttcctctgcg tgcgcgagga ggatctcaca cgcggcgatg acatccgcga cggtctcgac
    11101 cagttcccgg acaaagcccc gctcggcaac agcgtcctgc ccatcactta cgcctacaag
    11161 cccggccagg acgaggacgg cgtcaccgtg cgcgtgccgc tgcccgtggc cgagcatctc
    11221 accagcggcc agctccagtg gctcgtgccc ggattgcgcg aagaactcgt gctcaccctg
    11281 ctgcgcgctt tgccaaaggc cacccgccgc gcgcttatgc cgctggaggc tgccgcgcgg
    11341 gagatcgcgc gcgagtttga tcccggccgc gcggattttc tcaccgcctt cgccgccttc
    11401 ctgcgcgagc gccaccggct cgaagtccag gccgccgact ggcctgccga cagcctgccc
    11461 gcgcatctgc gcccgcgtgt cgaggtggtc ggcgcgcaaa acaaacccgt gattgccacc
    11521 cgtgacctgg gggaaatccg cgccctcgcc cgccagcagg agcgccgctc cgacgcctgg
    11581 gaaaaagccg ccgcgcgcct ggagaagcac gccatccgcg cctggtcctt cggcgatgtg
    11641 cccgagcagg tgctcatcga ggaaatcggc ggcgcgcccg tgcatggtta cccaggtttg
    11701 cagcgcaccg aggaaggcgt cagcctgcgc ttgttccgcc gcgcggagga ggcgcagcgg
    11761 cactcgcccg ccgccgtgcg ccagcttgcg gaggaggccc ttggcaaaga catcgcctgg
    11821 ctgcgcaagg aactgcgcgc cctggatcag cccgcgccaa agagcaccgg cctcgccagc
    11881 ctggccatgc tcgacttgag cgccaaagcc gcgcccacca gccccgcgga gctttccgcc
    11941 caggcctgcg agcacatcct cgcgcacgcc ctgcgcctcg agccactgct gccgctcaaa
    12001 gtggcgcgct tccgtgagat gtgcgagcgt gctcggcgag agttccccac actggcgcat
    12061 cgcgtgcgcg atctgctcaa gcagtgcgag gacttgaaaa agaaaatcct cgccttcccg
    12121 cgcccctaca ccggcctcaa agacgacctc gcccgcctca tgccgcccga catgctcacg
    12181 cgcacgccgc acgcccagct ccagcatctg ccgcgttatt tgaaggccat cctcctgcgt
    12241 gccgagcgcg cctcgaacca ccccgccaag gacgccgaca aagccgcgct catccgcgac
    12301 ttcgacggct gggagcgcga gtgcggcgag gcgaaccgcg aggccttccg ctggctgttc
    12361 gaggaatacc gtgtctccgt cttcgcccag gagctcggca ccgcgcagcc cgcgagcgtg
    12421 aagcggctgg aggcgctgct gggcgcggag tcgctggagt atcgtcaatg ctcagcatga
    12481 ttcgcgctgt ggcggagggt tactgtaacg gacatcccgt gattgcgctg gcatgggttt
    12541 gtcgttagtc atgggtcgaa ctcaaccgaa ccatgaaaaa gccacgcttc attctcgccg
    12601 cgctcgccgc gtcccttctt tccagccatg ccgcgcagca ggtttgggtg gaggccgagt
    12661 ccttcgccga ccacggcggg tggatgctcg acacgcagtt cattgacatc atgggcagcc
    12721 cctacctgct ggcgcacggc atgggcaccc cggtgaggga cgcgcgcacg acggtggcgg
    12781 tgacagaggc gggcagctac aaagtgtggg tgcgcacgaa gaactgggtg gggccgtggg
    12841 aggcgccggg cgcgccggga aaattccagg tgggtgtgaa cggggtgaaa ctggagaagg
    12901 tgctgggcac ggagggccgg gactggctct gggagctggc gggcgaggca cgcctcagcg
    12961 cgggccaggc ggagctttcg ctgcatgatc tgacggggtt cgaggggcgt gtggacgcgg
    13021 ttttgctgac tcaggacgcg ggcttcacgc cgtcggcgga ctttgccgcc accaacgcgc
    13081 tgcggcggca gtctctgggg ctgccacagg aggcgccgga gacggaggag tatgacttgg
    13141 tggtggtggg cggcggctac tcgggcatgg gcgcggcgat cagcggcgcg cgccaggggc
    13201 tgaaggttgc cttcatccag aaccgcccgg tgctgggcgg caacgggtcg agcgagatcc
    13261 aggtgtgggc catgggcggc acgcgccggg gactttaccc gcacctgggg gagatcatcg
    13321 aggagttcgc cgaccgcgcc agcaacagcc ccgccgcctc acccgaggag ttcaacgaca
    13381 agctcaagga gcaggtggtg cgcgcggagg cgaacattga cctttttctc aacacacacg
    13441 ttcacggcat caccatggaa aagggcgaca ctctcaagat ccgcagcgtc accggcctgg
    13501 acacccgcac gggcaaagag acccgattcc gcggcaagtt cttcaccgac tgcaccggcc
    13561 acggcagcgt gggcgcgctg gcgggcgcgg aaatcctcat ggaggaaaag ggccgcatgg
    13621 gcatgagcaa catgtgggtg ctgaaaaaga ccgccaaccc gaagccctgg cccgccaccc
    13681 cctgggcgct gccccttgag cagggcgact tccccgagcc gcgcgtgctg gagcctgcgg
    13741 gcgtgaagaa caaggccaac atgaccggct acgacctggg ctacaccccg gtggaaaacg
    13801 ccgaggatta cctgcatggc gagtggttct gggagagcgg ctttgatcaa gaccccatca
    13861 acggcctgga gcgcatccgc gactggaact tccgcgcggt ctatggcgcc gtgagcgcca
    13921 tgaagcgcta cgcgccggac aagttcggca actacgacat gacctggctg gcctatgtgg
    13981 gcggcacgcg cgagtcccgc cgcatcgtcg gcgacttcat cctgaagggc gaggacatgg
    14041 tgaagggcgt gatccagaac gacgcctgcg tgcccaccac ctgggaccag gacctgcatt
    14101 acccgaagga gcaatactcg gtcaagttcc ccgagaaccc cttcatctcc cgcgccgagt
    14161 tcggcaagca caccgaccgc aaaaacggct accccgtgcc ctaccgctgc ttttactcga
    14221 aggacgtctc gaacctcttc atggccggcc gctgcatcag cgtggaccgc tgggcgctgg
    14281 gcagcacccg cgtcatgcgc acctgcggca tgatgggcga ggtggtgggc aaggccgcct
    14341 ggatcagcgt gcgccaccac accacccccc gcggcgtcta tgagcagtat ctcgacattc
    14401 tcaaagacct catgagcaag cccggcgcca tgcgccgcga ctccctggag ggcgctctct
    14461 acctgcccgc caatgccaaa aagctgcccg aggtggtcag cgacagcatt gaccccagga
    14521 agctcgaagg catcgtcatt gacgacgccg acgccgagct gaccggcccc tgggccaccg
    14581 gcgagggcct caaacccttc gtggcggacc actacagcta cagccaggcc aaggaggcca
    14641 gcgcgcgctt ttccttcgcg gtgaaggaga ccggcaaata cgaggtcttc atctactggc
    14701 agccgcacgc caaccgcgcc aaggccgccc ccgtcagcgt gctgagcgcc gggggtgaga
    14761 agaccttccg cgtgaaccag agccagcccg ccgccggcaa gcagggcgcg cacagcctcg
    14821 gcaccttcac cttcaacgcg ggcgaggaat ccgccgtcac cttccgcacc gagggcagcc
    14881 agggcaacgt ccacctcgac gccgtgcagg tggtgcgcgt gccgtaagcg cgggtgtccg
    14941 gacctagccg gatgatccgg tgtctcgaag gagttcggcc ttggcggcag aacgggagga
    15001 atcgccgcgt attcctcacc ccatgcactc atccacccgc cgccgtcttt ttgacccccc
    15061 ccttgtgtcc gccgcagcgt cagttttgtc gagaatcttt cttttcaccc tgttcgccgt
    15121 cagcgctgct ttcgctcaaa acgacctcaa accgctccgc gtgggcattg tcggcctcga
    15181 cacgtcgcat gtgccggcgt ttacgaagct cttcaacaaa ggcgagacag agggcgagct
    15241 ggccggcatc aaagtcacca caggctacac aggcggcacg gacatgcccg ccagcgcgaa
    15301 ccgcaaagaa aagttcacac agcagcttcg cgacatgggc gtggagatcg tgccgacgat
    15361 tccggaactg ctcgccaaag tcgatgtggt gctgctggag agcgtggatg gccgcattca
    15421 cctccaggag gcgcgggaga ttttcaaggc gggcaagccg gtgttcatcg acaaaccact
    15481 cgctggcacg ctggcggagg ccattgccat cgtggagctg gcaaagaagc acaatgtgcc
    15541 ctttttcagc agctcgtcgt cgcgcttcgg cgcggagatg gtcgcgctca aggcgaatac
    15601 ggatcttggc gacatcctcg gtgctcagac ttgggggccg tgttcgtatc aggagggcac
    15661 gccggacttt ttcttctacg gcgtccacgg cgtcgaggcg ctctacacgc tgatggggcc
    15721 gggttgcgag accgtttcac gagtgaaggc cggcaaccac gatgtggcca ccggcgtgtg
    15781 gaaagacggc cgcgtcggcg cgtatcgcgg catcgtgaag ggaaaggcgg acttcggcgc
    15841 cgtggcctac ggcagcaaag ccatcggcca ggccgcgaaa gcggtgagct acgaaggtct
    15901 gtgccgccag atcggcaaat tcttccgcac cggccagcca cccgtcagcg ccgaggagac
    15961 gatcgaaatc ttcaccttca tggaggccgc agatgaaagc atgcgccagg gcggcaaacc
    16021 cgtcgcgctc gctgatgtgc tggcgaaggc gaaggcggaa gcggcgaaac tcatcaagta
    16081 agctcatgcg cacactgctg ttccttttca tcgggctgtt gggtgctctt gctgccgaac
    16141 cgcttcaagt gagcgtttca aacatccgcc gcgtcttcca caatggcgag cacaacgcct
    16201 tcaccgacct cgtgcgcttc aatggcgcgc tctacctgtg ccttcgcagt tgcccggacg
    16261 gtcacatggt cttcaacacc gcctcggtga tcgttttgcg cagcacagat caaggcgcga
    16321 catggcagcc ggtgcatcgc ttcagcgtga aggaccgcga cacgcgcgat ccgcacttcc
    16381 ttgtcttcaa aggccggctc ttcgtctatt caggcacttg gtggaccggc cccggcctgc
    16441 tcgaccgcaa ggactacgac atgaacaagc agctcggcta cgccgtgtcc tccgccgatg
    16501 gctcgaagtg gtccgaaccc accctcatgg agggcacctt cggctactac atctggcgtg
    16561 cagcctcgca cgacgggaaa gtgtatctct gcggccggcg gaaggccaac ttcgccgtcg
    16621 aagtcatggg cgagcgtggc cacaaccaat ccatcatgct cgaaagcgat gacggcatca
    16681 tcttccgcca tcgcgcctac ttccaggagt ccggcggcaa cgaaaccgcc ttcttctttg
    16741 agaaggatgg cagcgtcctg gccctggatc gcaatggagg aggcaactcg atgctgtgca
    16801 gttccaaacc gccctacacc gagtggaacc gctacccgct cgaccgtttc gtcggcggac
    16861 cgctgctggt gaaatggggc gagcacgttc tgattggcgg tcgtcatcaa acgaaggata
    16921 gcggcccgaa aacctcgctc ggctggctcg ttggcgacaa attccacgag ttcgccgaac
    16981 ttcccagcgg cggcgacaat tcctatcccg gcttcgtcga actctcgccc accaagggac
    17041 tcatctcgtg gtattcgagt catgagaagg acgaggcggg caaaacgatc acggcgatct
    17101 atttggcgga gctggtgatc agtcgttagc cggggcgatc atctcgcagc caatgccttc
    17161 cccagcaaat cagccacgcc aagctcggca gcactgttct ggagcagttc cagatcgagc
    17221 ttgcccgcat tgagcctcaa cacgccgagg atgtcgcccc attgacgctc ggagtcccca
    17281 ccaccgagcc ggtaccactc caatttggcg atgacgatgt cctccgcgca gcagacgcgg
    17341 gcttcgatgt ccgcaccttc cagtttgagg ctctgagacc tggcaaactc gccaccatca
    17401 aagcggcgca gtttgggaac gaagacatcc accttcatcg cagtttccaa gtggatgaga
    17461 ttaaaagagg agcgatcccg aatggcgtcc tgcatcgcca cctcgtccgc ataccatgcc
    17521 tctccgagtg cgcgcagaaa gggacgcaca tgttggggtc ggagatcggc catgatgtcc
    17581 gcatcaaaag tcgcacgagg aatgccgtga atcgagctgg cgagcgatcc gccgagaaac
    17641 cagcgcacgc ccagcttttc cagctcggtg gcagcagcca gggcagctcg caaggtatca
    17701 tccatgactg atgaacggac cgtaggcttt gtgagccagt tcttctccga gcaaacgctc
    17761 cgccagcagg cgatgaagct cctgcggcgg caattcagga tgctgctggt gtagcgaact
    17821 ccgtgtcagc tctctcaccg actggctcca gccaagcgcg aggcccaagc ggcgagtcgg
    17881 cgtcatcgcc tgcaaggcgc gcaggcgcat ggcttcagcg gtggaagagg tgtcgttcat
    17941 gacgccagac tgccatcagg ggccagattg gcaactctca gcatgcgtga accccattcc
    18001 agccagcaaa ccgagtcagc tcctcctgta ttcccccgcc atgctcacac tttgccttca
    18061 cctcctgttg gcatttctca gcaagaaaca caggacagag gactactcct tggatatctc
    18121 cgccagttca tgaaacgcct gcatcagcgg cttggctgaa ggacgtggaa tcacgcctgc
    18181 acgaggcaac tttacatagt ctgctttgat cgtctcgatc agggcggagc gatagcgtgg
    18241 ggattcacgc tttgttagtc cgagcggaag ggactgcacg acgtagtggt cgggaaactg
    18301 tttcagtgcc tggatgagtt gaggagagtg cagcgtaagt ttttcggctt ccatctgccc
    18361 gctgagatac gaggcggcaa agcgggcatc gccagtctgc ttccagtctt ggagatcgat
    18421 ggattcaagt tcggggctgt cgagatggag ccacgccagg gcattgtgca gttccgggtc
    18481 cttgctcttt ttggcctggt ctcgcatgac ggcaccccaa ttcttggtga gtgtctcttt
    18541 cgggaggaat tcgtgatcgt gaatgtaggt gatcaggaac gagagcacac gaccgtttgc
    18601 cggaagcacg ggttttctca gcaacagtcc ggcgtctgac aggatttgct tggccttctt
    18661 gggctcaagg tcttcggtca ctaattttgc agccaagagg gccagttcga gttcccgccc
    18721 cggctctttt aagccggcaa aggtagccag atcgacttgg aggaagtttt tgttgggtcc
    18781 gctgttccac cagtcctcag gccattccaa atccagttcg atcacgccga ctgccgcgtc
    18841 gaaatcaccc gctttggccc tggtcatgag cgcttctcgc tcccggtgct tgggctgcgg
    18901 cccgtgaatt ttgcaaactt ctgtctcaaa ttcctcgagc gtgcctgtct gcgccttttc
    18961 ggacttttcc cagagttcca gggcctcgtc cactttgccg agtctcaggg cgcactcggc
    19021 tgcgagcccg taagtgggtg cgtagtcctt catcaggcgg gaagcgacac ggtaactcgc
    19081 cagcgcacct ttccactctc cgcgccggtg ctgctccacc gcccacaagt agttgggcat
    19141 ctccagatct ggatgggccg cggccacttc tttgtgaagt tcgtagctct cctccgggtg
    19201 aagctgaaac atgaagctcg ccagcgcctg actctgatcg accgtccgct ggtctttggg
    19261 gtggacagaa ttcagcaagc gaacggcttc ggtccatttt ccctccgcta gcagttgatt
    19321 cgcctcaggg attttcagat cgatctcccg ataagcttcc gcgacggggc ctttgtgttc
    19381 ttcacccagc aaaaacgatt tcagagaaag acagatgaat agggcgagac gcttcatggg
    19441 cgggtttaag ctgaatttcc aaagctcatc acatcaggcc ttccatttca aggttttcga
    19501 ccagcctctg tgggagaact gtcccccgat cccgtattcc ctcccaccat gctcacaccc
    19561 acacgccgac gcttttttga agactcgctc atggccgctg cggcggtcgc tttgccgaaa
    19621 actttgctgg gggccgagac gacgacggtt tcggcaaatg agaagatcac ggcggcgatc
    19681 attggctgcg ggattcgcgg gaaggcgcat gcacgggagc tggcgaggct gccggagtgc
    19741 gacgtggcgt atgtttgcga tccggacctc gatcgcgcgg atgaggtggg cgcgttgctc
    19801 gtcgagttga agcgtccgat gccgaagaag gtgcaggact tgcggaaggt gctcgaagac
    19861 aaagccgtcg atgtcgtctt catcgccacg ccgaaccact ggcatgcgct ggcggcgatc
    19921 tgggcgatgc aggcgggcaa ggatgtgtat gtcgagaagc cggtgagcca gaacgtcgag
    19981 gaaggccgcc gcatcgtgca ggtggcgcgg aagctcgggc gcatcgcgca gacggggacg
    20041 cagaatcgct cgcgtggtgc cttggcggag gcggtgaaat tcatgcgcga gggcaaactg
    20101 ggcgaggtaa agctggcgaa gagcatcatc tacagcggac gcggcagcat cggcggcccg
    20161 gcggagtgtg tgatgccgcc gaggtgcgat tacgacctct gggccggccc cgcgccgatg
    20221 acaaagctca cgcgaccgaa gttccactac gactggcatt ggttctggaa caccggcaac
    20281 ggcgagatcg gcaacaacaa cgtccactcg ctcgacatct gccgctgggg cctcggcgtg
    20341 atcggtttgg gccgcagcgt gctcagctac ggcggccgcc tcggctacac agatgtcgcg
    20401 gagacgccga actcgcaggt gggcatcttc gacttcggcg acaagaccat cgtctccgaa
    20461 actcgcggtc tgaataccgc gccgtttcat cccacgatca aatccatgtg gttcttttac
    20521 ggcagtgagg gcatcatcgc cgacacgagc ctcttcgatc ctcaaggcaa gctcgtccgc
    20581 gccttcgagg gcaaatcgga gaatcacttc gccaacttca tcaaagccgt gcgtagccgg
    20641 agtcacaccg atctcaccgc cgacatcgag gaaggccatc aaagcaccgc gctctgccac
    20701 atcgcgaaca tctcgtatcg actcggctcc aaaacctcag tgaacgacat ctcgaaggct
    20761 ctcggcgcga tccaggtcca cgaagacgtg cagcacacgc tggagcgcac gcgtcattac
    20821 ctcgccgaag ccggagtcga tctcgacaag acccagctca cgctcggcca gcatctccgc
    20881 gtggatggcg acaaggaatc cttcctcgat aacgctgccg ccaacgccct gctcacccgc
    20941 gagtatcgcg cgccgtttgt ggtgccgaat ctgagcgcaa tctgaggcaa ctcatcaaca
    21001 tcctgacctt acctcatgct gcggctgttt tggattctcg gcagttttgc cttcgcctgt
    21061 tccttgggtg cccaggatat cacactcgag gcgcggcggt tgcatctcgg caaggccgga
    21121 gaatgggagt gggagatatt caaagatgtg cctgtggatg ccgggaagat ggagcttcgt
    21181 tttcaagcca acgtgaacac ctccgagcac acactgcgca tctggcagcg tgatgtgaaa
    21241 caatcgtggc ccgtcatgct gaatggccgc aggcttggcg tgctcacgct tgcagagacg
    21301 gcccttgagt gtgtgctggc ggtgccgcca ggtgcgctgc gcgatggcaa gaatgtgttg
    21361 ctgatcgact cgcccgcgcg cctcgatgac attgagatcg ggccgatgca tctcaccttg
    21421 aagcctgtgg cggaagccat tggtggggct gctttgttga tcgaggtcaa ggatggcact
    21481 catggcgagg cgatgccttg caggctcact ttgacccggc cagacggcac cttgcagcct
    21541 ttgcaggccg cgccggccgg cgatgtcgca gtccgtgtgg gcgtgattta cgcgaaaaat
    21601 ggcaaggcga gagtctcggt gcctccaggc gattacatcc tccatgcggg gcgtggcttt
    21661 gagtgggggg tggcgagccg caagctcacg atgaagcagg gcgagacgct gcccgttgac
    21721 ctgactctga gtcgagaggt ggatacaaaa ggctggatcg ctgccgacag ccacatccac
    21781 acgctcacac acagtggtca tggcgatgcg aagatcgaag aacgcatgat caccatcgcg
    21841 ggtgaaggca ttgagctggc catcgccacg gatcacaatc atcacaccga ctacgccccc
    21901 agcgccgcca gcgtcggcgt gacagatcgt ttcacttctg tcatcggcaa cgaagtcaca
    21961 accaagcacg gtcacttcaa cgccttcccc attcagcccg gcgcagccgt ggcaaatcac
    22021 aatgagcagg actgggcgaa gctcctgcct gccatccgcg ccacgccggg tgtgaaggtg
    22081 atcacgctga atcatccgcg tgatctccac agcggcttcg tgccgctcgg cgggctgcaa
    22141 ttcaacccca ccacgggcaa acatcgccat gcggacgctc tcgacattga tgcgctggag
    22201 gtcatcacct ctggtgccat gcagtcagac atccatctgc tgtatcggga ctggttcgcg
    22261 ctgctgaatc gcgggcatcg catcgcggcg attgcctcca gtgacacaca tgacgtgaac
    22321 cgcttcatcc tcggccaggg tcgcacttac gttgcagcaa aagatggcaa tccatcgcag
    22381 ctcgatctcg atgaagtctg gcgcagctat caggaaggcc gcctgctcgt gagtctcggc
    22441 ttgatgacga cactcaaggt caatgaccgc ttcaccgtcg gcgatctcgc cactggactc
    22501 ggctcgcaaa tcaaagtcga ggccgtggtc agcggtcccg cgtgggtcac cgcagatcgc
    22561 atcgaactct acgccaacgg catcctcatc cgtgagcagt ccatcaagga tgagcatcgc
    22621 gctggtgaga aggcacgcat cacctgggaa ctgccgaagc ctgcgcatga tgttcacctt
    22681 gtcgccatcg ccaccgggcc gggaatcacg gaacctttct gggagatccc gcggccgtat
    22741 cagccatcaa gcaagtccct cactccccga gtcatcggct ccacaaaccc catctggctc
    22801 gactgcgatg gcgatgggaa gttcgcctcg gctttcgcca tcgcccaggc actcgtgaca
    22861 aagcttggca gcgattcagc ggccctgcga gaggctttga agaagcacga tgctgctgtc
    22921 attgtgcagg tagagtcatt gcttccaaac tcaatcggca aaccctaaga acacagaagc
    22981 caacctgaga accgaatttc gcccacccat ccatgcagcc ctccgtcgtt cttcaaaaaa
    23041 tccgctccgg ccagaccgtc atcacggcga agtcatgcta caccgaccct gagctggtcg
    23101 aaatgatcgc ctcgtccggc tttgatgccg tgtggatctg cctggagcac aagcgtgttg
    23161 atgcggcgat ggtttatgcg cttatccagg cctgccggct cggcggcgcg gacgcgttca
    23221 ttcgcgtgcg gtcatccaac tacacagatg tgctgcaact catcgaggct ggtgcgcgtg
    23281 gcatcatgct gccgcgtgtg aagcatcccg ccgaggcgcg cgaggtggtg cgtgccatga
    23341 agttcccgcc gcttggagcg cgtggttttg atggcattca tgccgaggcc gattttggcc
    23401 gtatccagcc tgcggagtat ctggcgaagg cgaatgacga gaacttcctc gcggtgcaga
    23461 tcgaggagcc agaggtggtg ccgcatattg atgagatcgc ggcgattcca ggcgtggatg
    23521 tgctgtttgt cggcccggcg gatctcacgc tgggtcttgg caagttcggc aagacggacg
    23581 accacgaggt gcgggccatt ttggagcagg tcgtagccgc gtgccagcgc cacggcaagg
    23641 tcgcggccat tccgtgtgcg ccggagcagg tgaagagcta tcacgcgatg ggctttcgct
    23701 tcttcaatgt catcagcgac ttccgctgtg tctccaccgg catgaaggca gcccgcgcgg
    23761 cggtggagct atcatgaact gaagtctcca gcagattcaa ggaggccagc agattgtctt
    23821 ttttggatct ttaaatctgc tggcctccct gaatctgctg gaaaaactga tcaaaacatg
    23881 cccgtgacta ttcacacgct cgaaacagac ctgctcgtgg ccggtggcgg catcgcgggc
    23941 gtgtgctgcg cgcttgcagc ggcacggctc ggtgcccgcg tgatcctttg ccaggaccgc
    24001 agcgtgctcg gcggcaatgc gagcagcgag gtgcgcatgc acatcgtcgg tgcgacgggc
    24061 ctcactggcg gcgaggaact gcaaaatgag ctgcgcgagg gtggcatcat cgaagaactg
    24121 cgtctcgacc tcgccgtgca gaatccgcag cgctcgcccg cgctgatgga tctgctgctt
    24181 tacgacaaat gccgccgtga gccgaacctc atgctttatc tgaacacgac ggtcgtcagc
    24241 gccgtggtcg aggatgggat cattcgtgaa gtgcgtgctg aaaggccgtc cacggaggac
    24301 gccttcatca ttcgagcaaa aactttcgtc gattgcaccg gcgatggcag gctcggcatc
    24361 gaggccgggg ctccgttcat gcgcggacgc gagagcaaga cgcagtttgg cgaatcactc
    24421 gcgcaggacg agacggactc gaaaacgctc ggctcctcga tcatgttcca ggcgcgcaaa
    24481 cacgagcgtg cgatgctttt tgaagctccg ccgtgggcgc gacgcttcag cgcggaggat
    24541 ttcaagctgc gaccctacgg tcagagcggc ttcgacctcg gtctcgaata cggctactgg
    24601 tggatcgagt ggggcggctg tctcgacacg ctgaaggaca acgagagcat ccgcgatgag
    24661 ttgctcgcca tcacgctcgg tgtgtgggac ttcgtgaaga accactctgg cctcgacgcc
    24721 tcgcactggg cgctggagtg gatcggcttc ctgcccggca agcgcgagag ccgccgcttc
    24781 atcggccagc acatcctcac ggagaacgat ctcatcacct cccgcccctt ccctgatgcc
    24841 atcgcttacg gcggctggcc catcgacacg catccgccag agggtgtcga tgcgccgcac
    24901 gagccgccct gcacgcagca tcatctgccc ttcatctacg acatcccact gcgctgctgc
    24961 gtctcctccg gaccgcgcaa tctgatgttc gctggccgca acctctccgc cacgcacatc
    25021 gcctttgcct ccacacgcgt catggcgacg tgtggcgtcg tcggtcaggg cgtcggcacc
    25081 gcagcggcgc tcgctttgag gcaaaacacg cttcccgctg acatcgcgga cgatgaagag
    25141 ctggtcacga gcatccagca gcggctcttg cgcgatgact gttacttgat cggtatttca
    25201 aatgacgacg cgcatgatct cgtgcgctcc gccgtgcata tacgcgaact ggatgcgtgt
    25261 gggctcgttc gccagaacga ggacagtctc ccgcattctg gcgaatgcgc ctacagcccc
    25321 gaaaacatcc gcagcggcat cacgcgcgcc gtcacaaagg tgcctcctca ccgccaatcc
    25381 cccggcctgc atcgctggat gagccgcgaa ctgcccgcca ctgttgagat ccgctggagc
    25441 cagcccgtga tgctccgcga ggttgtgttg attttcgaca ccggcttgca tcgtcttctc
    25501 acgctgtcgc aggccgacgg ctacacgcaa aaaatgctct ggggccggcc gcagccggag
    25561 acaatccggg attacaggct cagcgtcgaa actacggatg gctggcggga tgtcgcagtc
    25621 atccaaggca atcatcaacg ccgccgcgcc caccttttag agccttgcgc ggtcacggcc
    25681 ctgcgtgtca gcatcagtgc caccaacggc atggatcacg cccgggttgt cgaggtgcga
    25741 gcctatggct gagggaggcg cttttcagat cggcaccctg cgcagtctgc gcggctggaa
    25801 ttttctcacc cggcccggcg ccgcggatgc ttttcccgcc tcctccttga gcaggcgcag
    25861 ctctttttcc acttcggcca ggcggcggga ggtggcgtcg agttttttgg ccaccaggcg
    25921 tgccacgaac tcaaagagcg cagcgtgaaa ggcgggatcc tcctcgcgcg gatggatgtc
    25981 ctgcaggaac ttctggtcca ccgccaggca cagggcgcgg ttgctggcga tgaccgaggc
    26041 ggcgcgtttg tcgcggttga ccagcgccag ctcgccgaaa acgtcccctg accggctgat
    26101 ggaggccact tttttgtccc ccacgcgcac ctcgagttcg ccgctgagca ggatgtaaat
    26161 gcgcgagtca atggtgccct cgcggatgat gacatcgccc tcgtcgcatt gcaggaggct
    26221 ggatgagttg agcacctcgt ccagttgctc ctccgtgaag ctgttgagga agggcaccgc
    26281 gctgagcgcg gcgggcaact gcccctcctc atggatgtaa gcgtattctt tcatggaatt
    26341 cgcccggatt atttcatgaa ccggacatca agaacagagc aaacgccgtt ccgggagaaa
    26401 atattttttt cgcctaagtc ccgaaacagc ggtcgcccgc gtcgccgagg ccgggcacaa
    26461 tgtagcattt ctcattcaga ccctcgtcca acgccgcggt gaagaccggc acatccgggt
    26521 gttccgcgcc gagagccgcc agcccctccg gacagcttac cacgttgatc atgcgcatgc
    26581 gtcgcgcgcc cgccgccttc aactgcgccg ccgccgcgca ggcgctgccg ccggtggcca
    26641 gcatcggatc gagcaggaac acatccgcct cggccagcgc ggcgggcaga ttcgcataat
    26701 aaggcactgg tttggccgtc tcctcgtccc gccggatgcc aagatgggcc accaccgcgt
    26761 cgggcaccag gggcaggatc gcctcggaga gacccaggcc cgcgcgcaaa acgggcacaa
    26821 gcacgatggg gcgcgccaaa gccgcgcccg ccgtctccgc cagcggcgtg agcacgcgca
    26881 acgcggaggt gtccaggctc cgcgaggctt caagaaaaag catctcagca agccggtgca
    26941 gatgccagcg gaagcgccgg ggcggcgtgt caaagcggcg catctccgtc aaatgtgaca
    27001 gggcgagcgg atgcgtgagg atgacaggcg ggttcatgcc ctctcttaaa gcgcggcagc
    27061 gcgcgcgggc aagcgtgcga tgacagggat ttgccgccac tgaaaaacga ccttgcagcc
    27121 ttgaagagcg attgtgcagc cttcaaaaat gactttgcaa gcagactctg gattcattca
    27181 tcacggtgca agaggatcaa attgagatct ttgacgatga tctgatgatt ttgacccggg
    27241 aacctcccgc cggaagccgg cggtgagggc gcccgctgga aacagcgtag gagagggcag
    27301 cggctggcta ccgcgctttc ttcgcctttg gttggtcgta gcggttactg tttatgaccg
    27361 atgaaaagtg atcgaagctt tggggggctc ggagacagcg tgctcagcca agcactggat
    27421 gctggctgtg agcagctggt atcgccgcaa aaacgggggg agtcgaaacg agggagtagt
    27481 ggcgaaggca tttgacgcgc ccatcgaggt aagcggtgca tctgcaacac catggcgaca
    27541 ggtccgcgcc cgccgctctg cgtcacgatg agtcgtgagc gtgaactcca gagacaatgc
    27601 cacgactaaa ggcggagtga tcagacagag tgaatgcgca atatctcgat gctgcttgtg
    27661 gtcacggcag gcataaagct gcgaaagcat agactccgca agccgctctc catgcgcgat
    27721 cctgtgaact ggccacccct ggccatcgtc tgcgattgta accgacgtct cctgagtgat
    27781 gatggagaga tgcgatgcat ctgccgcagc taagctcggc caacggatgg caccacgctg
    27841 atcgaagcaa gtgatctggg atgctgcaca cctcagcagt tcggtcatac acgctgggac
    27901 ggactcgaat agcctccaaa gctgataaaa agcaggccgc cacccatcgc cttacttctt
    27961 tttgccgcgc ttgggcctgc cgtaagtgac tttttttggc ttgggcggga cgccaccgtc
    28021 atcgggactg aggccggatt gttttttcaa ctcgcggatc tggtcgcgca gtagagcggc
    28081 gcgctcgaat tcgagtttgg cggcggcttc ggccatttcc tcctccaact cgcggatgac
    28141 ttcggtgaca gcgaggtcgc tcccgccgcc ttcgcggaca atgttttcct cggtctcgcg
    28201 tgctttgccg aggatttgca ggctctcctg cacggcgcgg cggacggtct ggggggtgat
    28261 gccgtgcttt tcgttatgct ccatctgcac ctggcggcgg tgggcgctga tgctgagcag
    28321 ggcctggatg ctctgggtct ggaggtcggc gaaaaggacg acctcgccgg cgacatggcg
    28381 tgcggcgcgc ccggcggtct ggatgaggga ggtctcgctg cggaggaagc cttccttgtc
    28441 ggcatcgagg atgcagacga ggctgacctc cgggaggtcg aggccttctc gcagaaggtt
    28501 gatgccgatg aggacatcgc agtcgccttt gcgcagggag cggaggattt cgacgcgctc
    28561 gatggcgtct atgtcgctgt ggaggtagcg gactttgagg tcgagattgc gcaggtaatc
    28621 cgtgaggtcc tcggcggtgc gcttggtgag ggtggtgacg aggacgcgct cgccgcgctc
    28681 gatgcgctgg cggcagagtt cgatggtttc atcaatctgg ccttggaggg gcttgacggt
    28741 gatgacgggg tcgagcaggc cggtggggcg gatgatttgc tcgacgacga gggatttgcc
    28801 cctggtggcg acgtcgaatt tgtccacggg ctgggcgttg ccgctgacgc ggatgctgcg
    28861 tccggggagg gcggcggggg tggtctcctc ctggccgatg cgctcgcggg cgtgggggat
    28921 gtaggtggtg ttgccgacga cgctgttctc catctcgaag cgggcggggg tggcgctgac
    28981 atagacgagc tggccggctt tttccatgaa ctcggggaat ttgagcggac ggttgtccag
    29041 cgcgctgggc aggcggaagc cgtgctcgac gagcacggtc ttgcgggagc ggtcgccctc
    29101 atacatgccg ccgatttggg ggatggtggc atggctttca tccacgaaga cgaggggtgg
    29161 ctcatcgaag aagtcgagca aggtgcctgg ggtggctccg ggctggcggc cggtgaggtg
    29221 gcggctgtag ttttcgatgc cctggcagaa tcccatttcc tgcatcattt cgatgtcgtg
    29281 ctcggtgcgc attttcaatc gttgggcttc gaggagcttg ccctcctgct caaagaaggc
    29341 cacgcgctgc tccatctcct cgcggatttt gacgatggct ttgcgcagtt tgtcaccagg
    29401 ggtgacgaac tgcttggcgg ggaagatggt gtagctgggc atcttcaggg tgacgtgacc
    29461 ggtgaggatg tccacggcgc tgatgcggtc tatttcatcg ccgaagaact cgatgcggat
    29521 ggcctcgctg tcgaggtagg cggggacgac ctcgaccacg tccccgcggg cgcggaaact
    29581 gccgcgcttg agctgcacgt cgttgcgctc gtagagcatg tccacgagct tgacgagaaa
    29641 ggtctcgcgg gacatttgct ggccggtgtg gatggggacc atcatgccct cgtagtcttc
    29701 gggggagccg aggccgtaga tgcaggagac gctggcaacg acgatgacat cccggcgggt
    29761 gagcagcgcg cccatggtgg agaggcggag gcgctcgatc tcgtcgttga tgctgctgtc
    29821 tttctcgatg taggtgtcgg tgcgcgggat gtaggcctcg ggctggtagt agtcgaagta
    29881 gctgacaaag tactcgactg cgttggcggg gaagaagttc ttgaactcgg agtagagctg
    29941 ggctgcgagg gtcttgttgt gggagaaaac gagggcgggg cggcccattt cggcgatgac
    30001 attcgccatg gtgaaggtct tgccggagcc ggtgacgccg agcagggtct ggtgccggtt
    30061 gcccgcgcgc agggacttga ccagtttggc gatggcctgt gcctggtcgc cgcgcgggtg
    30121 gaagtcgggg ttgagctgaa aactggacat ttccgggaca ggagcgcggg gagcgctttc
    30181 atgcaaagca aaggcgggtg attccgggaa gatcgcgtcg ttttcagcgc atggcgctgc
    30241 ggatcatgga gggggtggat gagcggaggc gggggatttc caatgcctgc cggattcgca
    30301 aatggcgctt tcaaaaactt gcgatgggga accgtgggcg gatagatttt caagtattcg
    30361 ttgtttttca cgcaagttcc tgtgccaggc cgcatccatc gggtcgaaac cgcatcacgc
    30421 catgaccttt cccaccaccc actggacgct gctggccgag gccacgctga acggcgatgc
    30481 cgccggacgg caggccctct ccaggatgtg cgaggagtat cggaagccgg tgctggcctt
    30541 tctgtcggcg cgcggctatc aggagggcga gcgtgaggac ctggcgcagg agtttttttt
    30601 gaactggctg agcacacgct cctggaagcg cgcggcgcgg gagaaggggc ggtttcgcag
    30661 ctatctgctg ggcagcgtgt gccacatgct ggcgcgtcat cacgagcgga agcacgcggc
    30721 caagcgaggt gccggcgccg gctgtgacag cctggaggct gcggctgaag cgggctggga
    30781 ggccgcggat gagacaccgc cggagtcgcc cgcctttgac agggcctggg cggtgacgct
    30841 ggtgatgaac actctgcatt gccttgagca ggattttgcg cggcggggca gggcggggga
    30901 gttcgcggtg ctgcggcgtt ttctgccttc gggtggcgag ggcatcactt tggaggaggc
    30961 ggcgcggtgt ctggacttct caaccagcgc ggttaaatcc gccgttcacc gcctccgcga
    31021 ggcttttcgc ctggcgttgc gcgcggcggt ggcgggcacg gtttccgctc ctcatgaggt
    31081 ggatgaggag ctggcttatc tgcgctcttt gctgctggcg ggtgtggaaa gccggaacct
    31141 ttcgaatgaa aaacggaaca tgtcttgagt gacgaatcca gaagccatga cctgcccgca
    31201 atgcggggcc gccattgacc ccgccgaggc gggaggcatg tgcgtggcct gcctgcttgg
    31261 ggaggcgatg cagccgcgtg tgtgccagga cacgctgggt gtcatcggcg gtcatgatct
    31321 gttggaggag ctggctcgcg gcggcatggg catcgtttat cgggcgcggc aggcggagtc
    31381 gggcaaggag gtggccttga aggcgctgcc gggcgcggag ctgctctccg aggaggcaag
    31441 gcgcagattt cacatcgaag ctctggcgat ggcgcggctg gagcatccct gcatcctgcc
    31501 ggtgcatgac acgggggagg acgacggcac gccctggttc acgatgaagc tggccaaagg
    31561 cgggacgctg gcgggaagga taacggagta ttccggcaaa tggcgggaga cagcggaact
    31621 gatcgcgcgg atcgcggagg cggttcagtt cgcgcatgag cgcggggtgc tgcatcggga
    31681 tttgaagccg ggaaacatcc tctttgacgc cgacgaccag ccaagggtgg ctgacttcgg
    31741 cctggcgaag ctgctggacg aggagatgga tctgacacgc accattgcgc tgatgggcac
    31801 gccgaattac atggcgccgg agctgacacg cggcggcaag ggcgcggcga cgaccgcgtg
    31861 cgatgtgtgg agtctgggcg tgatgctgta cgagctgctg gcaggccagc cgccgtttca
    31921 tggggataat ctggccacgg tgctgcgcca gcttaatgag gaggagcctg ccctgctgcc
    31981 gcgcgcggtg ccgtgtgacc tggcggtgat cacccgcaag gcgctgcaaa agacgcccgc
    32041 tcagcgctac gccagcgcgg gggcgctggc ggcggacctg cgccgctggc tgcgcgggga
    32101 ggccatcgag gcgcggccc
//
LOCUS       DAOMYJ010000031        31934 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Prosthecobacter sp. isolate AMR_MDS_5629
            NODE_512_length_31934_cov_20.828539, whole genome shotgun sequence.
ACCESSION   DAOMYJ010000031 DAOMYJ010000000
VERSION     DAOMYJ010000031.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867850
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prosthecobacter sp. (wastewater metagenome)
  ORGANISM  Prosthecobacter sp.
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Verrucomicrobiaceae; Prosthecobacter.
REFERENCE   1  (bases 1 to 31934)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 31934)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.30
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:34:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,742
            CDSs (total)                      :: 4,684
            Genes (coding)                    :: 4,676
            CDSs (with protein)               :: 4,676
            Genes (RNA)                       :: 58
            tRNAs                             :: 55
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31934
                     /organism="Prosthecobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_512_length_31934_cov_20.828539"
                     /isolate="AMR_MDS_5629"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1965333"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            236..3091
                     /locus_tag="PLP58_05285"
     CDS             236..3091
                     /locus_tag="PLP58_05285"
                     /inference="COORDINATES: protein motif:HMM:NF033679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNRLRE domain-containing protein"
                     /protein_id="HRJ07960.1"
                     /translation="MNRRPGCLLVCGLLLSAGICPAWESTLYGPGWQATPALSFEEDK
                     IVQDFSFAGYRRGEVPLPVTPPGLTYNAVTTYGADPTGAVDSTVAIQSAINAAAAAGG
                     GIVWLPAGLYKISPQGTNTYCLQINASGVVLRGAGTGQTFLLNTQTVMRDKNIISVTG
                     PSSAAWTTVQSPSTNITTDLPGPVTEIPVVSVAGFAVGDHILVRADPGDAWATEHLED
                     GWVGHAGSYGRLMYLRQIMALDAVNNVITLDIPTRYTLKTRDNARIYKKTTLITEVGL
                     EGFSVGNVQHPGTTGWGEEDYATAGTSAYDVHNSYVIRMSRARDGWIKNVHTYQPAGN
                     TTTCHLLNNGILLSECSRVTVKGCYFRRPQFGGGGGAGYMYRLQNSGDCLVRDSTAEF
                     CRHGFVFSHMSTSGNVLHACLDKTTGKQTGNTGNQNTSGKGSDHHMHFSHSNLVDACT
                     ADGSWFEARYRPFGSVPLHNLTAAHTVYWNTEGKGTAAAYVVHSQQSRFGYVIGTRGT
                     LTAVNTGGTSTTKTTPVDHVEGTAQGDTLTPFSLFREQRRRRIGLPTVAEMAETQALF
                     PNNTALVTPQVWFGDSTTAPPDAAFTWARTSGPGAADLQSAGGFAVNAAFLLPGAHTL
                     TLTVSRHGSLEEDWAASAPATVRVLPAGTVETELPPVADAHVQEGTADANYNTTVLWM
                     KYVGSSNPVNREIFMRFDLSSLAGRTVQSAELRLNSTEPDTAATGQTHFVANDTWSEA
                     GITWNTRPAVSTLLQTWPLATDYAQRIDLTAGTTAEAAGDGLLSLRHSIQSQTNSATV
                     FKYASREHATTALRPRLRVLHSEVWPSFTAWISGFPQIPAEFRDADDDPDGDGVKNLE
                     EYARRAQPHLAEALPAGLLAAREGGALRLRVPGGASVPPGVYPVLEHNAGLSPAGWQA
                     LMRVSVEVSGADLVFTVPSGLAQAGPGFFRLRYEAVP"
     gene            3189..4790
                     /gene="serA"
                     /locus_tag="PLP58_05290"
     CDS             3189..4790
                     /gene="serA"
                     /locus_tag="PLP58_05290"
                     /EC_number="1.1.1.95"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009958439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004617 - phosphoglycerate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HRJ07961.1"
                     /translation="MAQKLNILVADPISETGIELLKKEPSFSVEVNLGLKAEADFAEA
                     AKNAHAIIVRSGVKVTARVIEAAPNLKAVGRAGVGVDNIDVTAASKRGVVVMNTPGGN
                     TISTAEQAFTLMMSLARKTPQAHASIVAGKWDRKSFQGTEVNGKTLAVLGMGRIGAEF
                     AKRAKAFNMRVVAYDPYLSKNRAESLGVELFENLDEALGQADFITMHMPLTPDTRHML
                     NAERLGRLKKGARIINCARGGLIDDNALAQALTDGKVAGAALDVFEVEPPPADYPLLK
                     APNTVFTPHLGASTGEAQENVGIEIAEVIAAHLLHGTIVNAVNMPNVDPKTLAEKGHF
                     LKFGELLGRLLSQFAPARSGQITISYSGKVGTGDTSLISRAILKGYLERPCGTEQVNY
                     INATGVAENLGIRFNESRVPDASEFSDLIEVQAANGAETASIAGTFFAGSPRIVKVNG
                     RHIETRPEGTLLLIENIDRPGMIAAYSAALGKNQVNIADMSLSRNKEGGTALTLLTLD
                     STPSPEVIAELEAIPGISRVHCVVV"
     gene            complement(4803..6245)
                     /locus_tag="PLP58_05295"
     CDS             complement(4803..6245)
                     /locus_tag="PLP58_05295"
                     /inference="COORDINATES: protein motif:HMM:NF016612.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="neutral/alkaline non-lysosomal ceramidase
                     N-terminal domain-containing protein"
                     /protein_id="HRJ07962.1"
                     /translation="MKRTAPLFILAGILCLPPVNAPAQESAERVFKAGAATSNITPPL
                     GMEIVGNFAPRPIAAHVHDELHARCLVLDDGSTRLALVVADTISLARDVWDEAKSKIE
                     AATGIPAAHMMFSGTHTHSSVSAITDPDSSLNYRSLIISRVVDGVRRAVNNLEPARIA
                     WGAGAVPQHVFNRRWLLKEGVTNTNPFGGQDRAVMNPASLLRPRMAGPAGPVNPEVYC
                     LSVQAADGRQIAIMANYWLHYVGGVPREHLSADYFGEFCRVIEKELGSDDAHPPVVGI
                     LANGPCGDVNNNDYSGKTPDVQRAPYEKIKLVAGDLAAEVLRVRGALKHQDWVPLKAA
                     VEDLELAARRPAPEMIEWAKGVLAKPRDAEPAHRLEQPYAERVLAARDSKADTVSCVM
                     QAFRVGDLGIAAIPFEVFTEIGLEIKARSPFKDTLTIELANGSNGYLPTPAQHELGGY
                     ETWLGTNRVEREASVKITAKILDLLGRVSQ"
     gene            6393..7316
                     /locus_tag="PLP58_05300"
     CDS             6393..7316
                     /locus_tag="PLP58_05300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ07963.1"
                     /translation="MPFPAATRFYARMTRVCAAVVMLPLLCARFPAFAGPETDGKDAF
                     TLEIRGKSIEVFTYRPKNFDGGRLIVVMHGMNRNARDYRDNAVTLGDRFGALIAAPAF
                     DLGQFPVEAYQRGGVTRDSKPQPESEWTFRYVWDVVGEMRRREDRPGMECRLIGHSAG
                     GQFLTRLAGFMPGDAARIIAGNPGNLLFPTHDMPFQYGFGGLPEALAGDDALKSYLAA
                     PLTLYLGLDDTGTANLDMTENAMRQGATRIERGRACFRMGEALAREKGWPFGWRLVEA
                     PGVAHDSKALFAHPQAAEALFGGSSGALEPH"
     gene            complement(7369..8625)
                     /locus_tag="PLP58_05305"
     CDS             complement(7369..8625)
                     /locus_tag="PLP58_05305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009964462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HRJ07964.1"
                     /translation="MSTRVVILGAGVVGLCTALYCARRGMSVTVIEKKPRARDGCSFG
                     NAGMVVPSHFIPLAAPGMMRLGLKWMLNPESPFYIKPRPDPSLLAWGLRFWRASTQKR
                     ADAAAPVLRDLGLLSRRCFEECGIDTGLVKRGLLMLCKEPRALEDEARFAEKSRALGM
                     PAQVLDAKATATLDPALTLDVAGSVHFPLDCHLAPGRFIAALEKELEFLGVNFRFDTE
                     ITGWSARALAGGRREIFRVFCGNDELPVDELVMAAGVWSADCAREFGLRIPMQAGKGY
                     SLTLEKPRQLPSLCAICTEARLAVTPMDGALRVGGTMELAGVDESITRRRVRGIIREF
                     TRYYPAFHEDDFSSVTPWSGLRPVSPDGMPYIGRARAWANLTVATGHAMMGLSLAPAT
                     GMIVAGLLAGEEPPVPLDLMSPDRFR"
     gene            complement(8704..9462)
                     /locus_tag="PLP58_05310"
     CDS             complement(8704..9462)
                     /locus_tag="PLP58_05310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ07965.1"
                     /translation="MNLLPHFVIALCALSLTDLQARDEAAWLSENGALIFHDAFERQE
                     DGNLAKAIGNGWNSATADRVPLLKQADLDEGILKIASATKEAGHAAHIHHAAGFQDGG
                     VVVRFRFPGINQGETLQLGFVDRETPGVHAGHLCYGILSQSSVTLIEQKTGVMDLGIR
                     ARRQDVLDRKEKLPADLEALLKSKQVVVPWKADNEWHELMLVTEGDELRLSLDGALLA
                     AHSSPGFAHPVKRWLSFLAGSTVWIDDVKIWRVR"
     gene            9603..10400
                     /locus_tag="PLP58_05315"
     CDS             9603..10400
                     /locus_tag="PLP58_05315"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HRJ07966.1"
                     /translation="MESNWKGKIVIVTGGGGGMGQAAAQRFAGAGARTFVFGRTLESL
                     QAAAAISPEIIPVVCDVADTASIASAMRVVLAEGAPFALIHTAGINTPDRFLDHADPG
                     RLVSEESWRRVMEINVLGVANMIQAVTQPMAAAGGGRIVVVSSTAGHGFDSYAGVPYT
                     ASKWAVHGMLFTARMQLNARGIVVSEYAPGEADTPLVKKRPVQPAAEHKSAMITCADC
                     ADALFFIASQPHSMSIIQLPAYQPFGGMPPQITAPWLAGLDIKPAGT"
     gene            complement(10533..11816)
                     /locus_tag="PLP58_05320"
     CDS             complement(10533..11816)
                     /locus_tag="PLP58_05320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009959339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-like beta-propeller repeat protein"
                     /protein_id="HRJ07967.1"
                     /translation="MNCPLHCLLGGSLLAAAGLSAQTSAPVEWGKFRGPNNDGSVPAP
                     QVSNWKGARVKTLWKAETPKGFSSFAVAGGKAFTLVTGEYDGNSGEMIVCLDVRTGKE
                     LWSKPLSIIGKYDGGGDAGTNDNKGGDGPRSTPVVDGDKVYVIDSSLGVHCFSAAGGD
                     LVWNRDVLKENAGVQIRWQNAASPVIEGNVLMMCGGGEGQALLGLDKTSGKVLWKGED
                     DKMTHATPVVADLLGTRQCIFFTQTGLVSVLPESGKVLWRQPYRFNISTAASPVVFED
                     IVYCSAGYGVGAGAYQISKSGSSWKSKEIWRKENDDFANHWSTPVVKDGHLYGMFSFK
                     KYGEGPLACVDIRTGDMKWSTPGFGPGQVILAGDSIIALSDKGELVVVEANPRKYTEL
                     KREEVLEGKVWSYPVLAYNRLFARSTTEGVCLEFQ"
     gene            complement(11929..12954)
                     /locus_tag="PLP58_05325"
     CDS             complement(11929..12954)
                     /locus_tag="PLP58_05325"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007417463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004073 - aspartate-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HRJ07968.1"
                     /translation="MSNLKHVAVVGATGAVGAEMLRCLEQRAFPVGRLSLLASARSAG
                     KKLKFKGEDVTIEELTPESFAGVDIALFSAGGGISKEFAPHAVKAGAVVVDNSSAFRM
                     DPAVPLVVPEINGGDAKSHQGIIANPNCTTAISLMALHPLHQAFGVRRIFASSYQAVS
                     GTGAQAIEELARQTREWAAENRAWDAPKLASQPHVYPHQIAFNAIPHVDSFLDSGYTK
                     EEMKMENEGRKIMHHPDFRASVTCVRIPVFRAHSIAISAEFEKPVTVEAARAVLAKAP
                     GLDVMDDPAAKQYPLALDIAGRDNCAVGRLRLDCALDNGLAFWVVGDQLLKGAALNAV
                     QIAELLV"
     gene            complement(13105..14403)
                     /locus_tag="PLP58_05330"
     CDS             complement(13105..14403)
                     /locus_tag="PLP58_05330"
                     /inference="COORDINATES: protein motif:HMM:NF039847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HRJ07969.1"
                     /translation="MKTRRSFLTQILATGVAPMFVPARLLRSQTAPSNKITLGVIGTG
                     AQGIVDMRAFLNHDDVRVTALCDVNTQNLERAKGHIAERYGSADVKSFADFRELNRDA
                     SIDAVLMALPVHWHSIPSADAVLHGKHIYHEKPMGMSIEESKLVRAAVRKKNVVFQFG
                     TQQRSDLKFRWAAELALNGRLGKLREIQVGVPGGKTMPAFPEEKTPAHVDWERWVGPA
                     PMTPFSGEKLKRDNHENITNFSLGMISCWGIHHLDIAQWGNGTDDTGPVSVEGEGTHP
                     DNGGCDAVLTWKMRFEFAKAAPITFANIGSMGLQMGARFIGENGWVHVTRGQISASDD
                     AILRDPQSKAGTMPVKLPVSLEHTRNFVDAVKSGKKPVCDIETAVRSDTLCQLAAVAL
                     KAGRKLAWNPASEDFEADSEANALMAARPFRGDWKLPQIV"
     gene            complement(14410..17235)
                     /locus_tag="PLP58_05335"
     CDS             complement(14410..17235)
                     /locus_tag="PLP58_05335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009963438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HRJ07970.1"
                     /translation="MTVSCLTFLLAASSAMAGPIRVLYIDSPGADTAPLHNAMRDLGR
                     DAIYFDYAKEKIDAAYDLTLTPSDLSDPSDLKARILGKLSPERKTAYEAFFAQREPEK
                     REKHPQVANYEKRPEPLTFQKPFSPKGSMERTQVPADCELKLFAAEPDIAKPIAFAWD
                     ERGRCWVVETRDYPHGVQESGEGQDSVKICEDTDGDGKADKFTVFADKLNLPTGIVFA
                     RKGIIVAAPPKFIYLEDTDGDDKADKRETIIDCWGIKDTHAQASSLHYGFDNWIYGCV
                     GYSGIEGYVGGKKQQFSMGTYRFKPDGSAIEFLHQFTNNAWGHSTNDAGDQFGGTANG
                     APLFFGGIPATAYEEGSRGMTAKKINVVDTCHTITPNFRQVDVFGGYTSAAGSNFIYS
                     NALPKRFQGMAMVCEPTMKVVSLMDVQPDGAGYKALDAFNLYASSDEWNSPVHAEVGP
                     DGAVWVADFQNFIIQHNPTPSKERGGFVATTGVGGAHENDLRDHSRGRIYRIVAKNAP
                     RYVGGPMPAPKTTSKNWVSELNQNTQHGRLLVQKVLVEEQAFIHSGKEELVPDLKAAV
                     SATEGKIGSIHSLWTLHGLGKLDAKTHQAALISKDAKLRRNAIRALGGDKAAQDLFFG
                     SGVISDPDLTTRLAAFVKLADFPTTPEIQTLVKKLAADPVVKSDEWLAEAAKMLGKKH
                     KVLNYQEGPNLLPNAGFEEPIAQTWKKRDYGNRPGNKGAEWGIVTDAKMVHSGKQAMR
                     CITRDDADTSFFADVPLKPNTDYRLSAWIKTHGFRGKASLNDHIGRAETSPKINRTQD
                     WTEVEVVFNSRNHPKASINLLHVGKGDVYFDDVKLCELVLENEDDASKLIGDIQRGEK
                     IFWEHPVAACKNCHLLKGQGSPVGPALDGIASKKDEAYIIESLVNPNAKLAEGYTATP
                     ISPMPPLNLILKPQEFADVKAFILSLK"
     gene            17398..18099
                     /locus_tag="PLP58_05340"
     CDS             17398..18099
                     /locus_tag="PLP58_05340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009963439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HRJ07971.1"
                     /translation="MAPESQFYLLFNHLPRVSFFAKDAQFRFVAANCHFVERLGFSCE
                     AELLGKGDFDVFPSRLAENFRRDDEEVLRSGEPKLGIVELFFNRQGIPDWFVTHKLPV
                     LNRKGSVMGIMGIVRAHGSAPDDAHPHRQIERAVEHIRTRFREKITVPELAEMAALSP
                     RQLNRKFVEAFGMSPQAVVMKVRIQAACELLAYPDKPVGEVAREVGFADQSSFTQQFH
                     RHMGQTPLSYRRQFL"
     gene            complement(18158..19612)
                     /locus_tag="PLP58_05345"
     CDS             complement(18158..19612)
                     /locus_tag="PLP58_05345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009958150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HRJ07972.1"
                     /translation="MALNIISRRHFLGRTAGLAASAFAGPNLLLRGQEAAGRRLNIAI
                     IGANGKGQSDTQAVTLDHNIVALVDVDMKRLEEAGKKNADHHLKSGKSEPRAPKLYQD
                     YRKMFDEMANEIDAVIVSTPDHTHFTAAMHAIKLKKHVCVQKPLCNYINEVRELHQAA
                     RQAGVVTQMGNQGRTMEGQRLAKEWIEQGAIGTLKEIRLWTNRPIWPQGPLAKKAAEC
                     PPNLDWDLWLSSEADESYFEFVLPEGVTNKRSGNSIHPFAWRGWWQFGSGALGDMGCH
                     IMDATFSVLGQLIPEKIDAVSSPISTTCAPVWSDLVYHMPAGKHPALKVSWNDGSKDG
                     MPNKPEISPHMTSDLAKKAFEKASSGMMFIGTEATVFEGEAYCSSPVIYNEERYTQVR
                     LDMKAEKIKKTEARSVLPNNPQGEWAWHIVNGGSPSSNFDYSCPLTEFVLLGNLAVRA
                     QQTVQWDKEGMKVPNAESANQFVTRPAYRPGWKV"
     gene            complement(19956..20156)
                     /locus_tag="PLP58_05350"
     CDS             complement(19956..20156)
                     /locus_tag="PLP58_05350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ07973.1"
                     /translation="MNLDWLNQILPILRCPDTRQELRWAASEELACEGLPPGEKALMR
                     KDGTRLFRIEDGIPNLLPGGMD"
     gene            complement(20222..21178)
                     /locus_tag="PLP58_05355"
     CDS             complement(20222..21178)
                     /locus_tag="PLP58_05355"
                     /inference="COORDINATES: protein motif:HMM:NF021492.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HRJ07974.1"
                     /translation="MSTPTAQPDSLEKLTPMEEFLEANFKKIVRACLALIALVVIYGL
                     ARYFGSVRDAEAAAAFAAAKTIEDYDIVIAERAGSDAAGNALLMKADLLWRDNKKDSS
                     IDTLNTFLAKHSGHPLESQAQLALASKFDSIGKKAEAKAAFEKLVAGNPGSDVAAVAQ
                     IRLGDILWAEGKEDEAKTVYESLPAKFSNASEDILNQGETRLKWIAAKLPTTEVDGPP
                     KPKEEAAPAAGAPQIKLDSGTLLSPGVSGLSTMPPINLTPSPAPKSATTPPVEVSPDK
                     PSAPAAPKKDEAKPAAPVPAPPAPAVPKSGDAPAEPAAPKTP"
     gene            complement(21206..22183)
                     /gene="cysK"
                     /locus_tag="PLP58_05360"
     CDS             complement(21206..22183)
                     /gene="cysK"
                     /locus_tag="PLP58_05360"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018290448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004124 - cysteine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HRJ07975.1"
                     /translation="MIYNNIVETVGKTPLVKLNKVTEGVNATIALKCEFFNPLGSVKD
                     RIGMAMIEDAEQRGILKKDTVIVEPTSGNTGIALAFVAAAKGYKLILTMPETMSLERR
                     TLLALLGADLVLTPGPQGMKGAIARAEEILKETPNAWMPQQFENPANPSIHMRTTAEE
                     IWADTDGSVDILVAAVGTGGTITGCVEVIKPRKPSFQAIAVEPEASPVINQTLAGEPV
                     QPGPHKIQGTGAGFVPKNLHLKDSAGNAQIVECVKVSNEDAFAMARRLAAEEGLLVGI
                     STGANVVAALRVAQRPENAGKLIVTIACSTGERYLSTALADEARAKVGA"
     gene            complement(22229..23395)
                     /locus_tag="PLP58_05365"
     CDS             complement(22229..23395)
                     /locus_tag="PLP58_05365"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009962470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HRJ07976.1"
                     /translation="MDFIAKNVAELSPSMTLSITSQAKALKKQGVDVLSFGAGEPDFN
                     TPAHITAAAIEALNKGQTRYTESAGLLELREAVAAKLLIDNNLQYDPSQISVNCGAKH
                     SCYNAIAATINPGDEVIIPAPYWTSYPEMVKLVGGIPVIVETKAENGWKMTADEFEDA
                     MSPMTKMIILNSPGNPTGSVYSREELQAIGEIAAGEDILILSDEIYEKLVYGENKHVS
                     IASLSKEIFDVTITINGFSKAYAMTGWRLGYTAAPKKIADAIDTIQSHTTSNPTTFAQ
                     YGAIAALSGDQQIVADMRDEFDVRRQYMLSRLQGIRNVRVVEPLGAFYFLVNIEPMGI
                     NSVNFCEKLLSKAKVAAVPGVAFGAENTIRFSYATGLDVINAGMDRFEEFCAQH"
     gene            23581..24621
                     /locus_tag="PLP58_05370"
     CDS             23581..24621
                     /locus_tag="PLP58_05370"
                     /inference="COORDINATES: protein motif:HMM:NF012472.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HRJ07977.1"
                     /translation="MERKRLGRSGIVVTDICMGTMTFGLQADEKTSFEIMDRAHDAGI
                     DFYDAAEMYPVPPSAERFGITEEIVGRWLKTRERGRVIVATKVTGPGHGWFRPPVRGG
                     FTALDRRQIHRACEDSLRRLQTDYIDLYQTHWPDHHMRQEDTLEALTELVEQGKVRAI
                     GCSNETSWGLMKNLWASEKHGLARFDSVQNNFSLINRRCESELAQVCRMEGVSLLPYS
                     PLGGGVLTGKYQNGALPPGARFSEYLVSGGERQQRMARRFVNERTLETTARLSVIAAD
                     IGVSVTALAVAWSRQHDFVASTIIGATSVAQLEQSLQARDLILDAETLRRIDEIDTAI
                     PNPMTEDGLRRL"
     gene            complement(24631..25782)
                     /locus_tag="PLP58_05375"
     CDS             complement(24631..25782)
                     /locus_tag="PLP58_05375"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HRJ07978.1"
                     /translation="MLETLQTTFGMLIAVRVFAMDDMGKSVFLSATSGGLMTSLFVVP
                     LLLRSRSTLAGTAARVQFLGGVFLALAALMPRSPWMFVAGLSLGLFCFSMQIPLLTQI
                     WRLNYPEAARGRLFSIAGVVRAAAAMGFGFFGGWLIGQDLGHYRWLLWIFAACAFISG
                     HLTRRLPSVVWSAREDTPPGLWAALRWVKKDRDFRTLLISWMMMGIGNLVAACLFVEY
                     LANPVHGINLPGRDVAWITGVVPVLFRMLCAYPWGVIYDKMGLFKVRAVLNLIFAAGI
                     LVFYLGENMFCWTLGMALWGVANAGGNVTWGLWVTKLAPEHAVAEYMSVHSFLTGLRG
                     VLAPYLAFAALGVMSFQTLGVVCSIGIIAASAFITSRSPADRSGARERL"
     gene            complement(25897..27690)
                     /locus_tag="PLP58_05380"
     CDS             complement(25897..27690)
                     /locus_tag="PLP58_05380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007415435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase"
                     /protein_id="HRJ07979.1"
                     /translation="MTAAAEVTNISCYLFAPLADLKSLRPRLLELCRAQGLKGTILLS
                     TEGINIFVAGDARGIETLLDFLRAMPGLARLAPKHSLSARQPFRRMLVRIKKEIIAFG
                     VEGIDPARHTSPRVSARELKQWLDEGRPVTLLDTRNDYEVRLGTFKGALPAGIDHFRH
                     FPDAVRRLPAGLKKQPVVTFCTGGIRCEKAAPFLEREGFEQVWQLDGGILKYFEEVGG
                     AHYEGECFVFDQRTGLDPALSETGSAQCHACMAPLTARDQDDPRFTEGVSCPYCFKTD
                     DERRRASLAARQEALRRAADPLPGSTPQDNHRPISIPARCHGMSLLDALCLLLPGVSR
                     PEWLMLFENQRMLAQDRQPASPERVVQGGERYLRLQPADVEPAVSADIRILFEDEAVI
                     VLRKPAPLPMHPGGRYHRNTLQHFLQTAWHPQKPRPAHRLDANTTGLVIAARTRHFAG
                     LLQTQFARGEVEKEYLARVTGHPAWEERLCDLPISGAPGELGSRDVDETAGLPARTAF
                     RLLRRDEDGTSLVEARPLTGRTNQIRVHLWRLGHPVCGDPVYLPGGALGVTQTLGVEA
                     PPMCLHAWRITFTHPLSGERLGFEDDPPSWH"
     gene            complement(27755..28489)
                     /locus_tag="PLP58_05385"
     CDS             complement(27755..28489)
                     /locus_tag="PLP58_05385"
                     /inference="COORDINATES: protein motif:HMM:NF028108.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5069 domain-containing protein"
                     /protein_id="HRJ07980.1"
                     /translation="MKTKSTLLIALVPGLAPFSTASAGLITDPLSVQAPISDQGLATD
                     PLGQGGPLPGPKPPVNVSAAAQFLADVVAAGGPGVGRQREARRSLADSFPCAIHPLMS
                     KPDPSTLPCSPLDETAGLKYFPRMTAKIRLHAQGRLWEDLHANLGKGSDGALAGFLHV
                     DYEDIKARVLQGGSDEEILEWCQERSRPLNDTDRLVWNHYVQTLGWNDHISAILEKRK
                     ADGGLERRDDIQTIAHYIDVDEGRRP"
     gene            28675..30909
                     /locus_tag="PLP58_05390"
     CDS             28675..30909
                     /locus_tag="PLP58_05390"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: protein motif:HMM:NF012585.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HRJ07981.1"
                     /translation="MKAFLCHNSNDQAFVDQVDKWLRRFGLGTFNYTREQCASKEWPA
                     QMREEARQCPAMVIFVGGKLSDPQKQEITTAWNESEVRVNAGKSRKQFVFVAVAAKAD
                     FKSVPHGTADPKFLPRIDYDPGRSSEQVARKIAFYFHVPTVVNGLPSNPRLFNYEKMI
                     LRHLENVEKHKGDLYLDHQKQDAKTSLLLEELRRLSLAGAPLEWPQVREIKEFMNGAP
                     VPNPWVGVEGSTGFDFGVVRTENHRVRMSATVDGSDGSHDAGKYTLPEAGPRGKLWFP
                     PKNAKGFKVGVLVSGGIAPGINAVIDGIVQRHWLYHTRNENGACPVIYGYQNGFSSLL
                     GPGRANQPYRLLAACKKHKDEYTSQGVHGRSNSIITSEYANEGGSIIGTSRVDELTSP
                     DSVEFLEDMARTLLNDEIDVLYVIGGDGSMRAAHALWTVALERMEKQEGKQRKQKILS
                     IVGVPKTMDNDILWVWQSFGFMSAVEKAREVIESLYTEVSANPRLGVVQLFGSVSGFV
                     VSHAVLASSTGHCELALIPEVPFSIEGVAEHMRKKMAANANAKGHDMPFGLIVMAETA
                     IPTDVDKYLDLPDKPASKDEPDPHAVYRVELTDEEEKALKDYLKNKNIISGQTSDSLR
                     RAGLKLMSTGLANLLKSTPETNGLQWQNLRCLTNEPRHLLRSAAPSCSDIINCQRLGT
                     LAVDNAMGGYTDFMISQWLTEFVLVPLSLVVLGRKQIYTPGIFWKSVIAKTGQHESDI
                     NQVS"
     gene            31061..>31934
                     /locus_tag="PLP58_05395"
     CDS             31061..>31934
                     /locus_tag="PLP58_05395"
                     /inference="COORDINATES: protein motif:HMM:NF012770.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HRJ07982.1"
                     /translation="MADHTLTLSNGLTLGYAEYGDPAGAVMFYFHGWPSGRVQGELMH
                     EAGLKFGLRIIAPDRPGIGLSDFQPRRRLLDWPPVLEELAAHAGAEKFHVLGWSGGGP
                     YVLATALKMPARLLSATIVCGAPPLAFFGDAKMFWLYRLMIRLRGHFPSVLGAVLRLG
                     ALLARGSPDKPPLKWLMRLLLEPDRRVLADPEVFRVVRAGMMESLRRGPRAVIGDADI
                     YLSEWGFEVSSVTFPVHLWHGKMDRNIAWEYSAQLAELMPHADVHWLENEGHYSLPIL
                     HNEAILRHALGPGAA"
BASE COUNT         5721 a         9652 c        10541 g         6020 t
ORIGIN      
        1 ggcgccgctt cttcaaatgc ggcgtgcgcc agtacttcga gggccagcag aacggtcagg
       61 gcgcggatca tggaatgaat aacgctgccc gccgcattcc cctcgccatc cattccacaa
      121 atgacagggc gcgatttgga ggagcgggcg gatgattcga gcggcgatgg attcaacttg
      181 ccgttccgag cagaggcgcg ttaaaaggcg ggaaatgccg attgtctcca agatcatgaa
      241 ccgccgaccg ggatgtctgc tggtgtgcgg actgctgcta agcgccggga tttgcccggc
      301 ttgggagagc acgctttacg gtccgggctg gcaggccaca ccggcgttgt cgttcgagga
      361 ggacaagatc gtgcaggatt tttccttcgc aggctaccga cgcggcgagg tgccgctgcc
      421 ggtgacgccg ccggggctga cttacaacgc ggtgacgacc tatggcgcgg acccgacagg
      481 cgccgtggac agcacggtgg ccattcagag cgccatcaat gccgcggcgg cggccggggg
      541 cggcatcgtg tggctgccgg cggggcttta caaaatcagc ccgcaaggga cgaacaccta
      601 ctgtctgcaa atcaacgcca gcggcgtggt gcttcgcggc gcggggaccg ggcagacctt
      661 cctgctgaac acgcagacgg tgatgcggga caagaacatc atcagcgtca ccggcccctc
      721 cagcgctgcc tggacgacgg tgcaatcgcc ctcgacgaac atcacgacgg acctgccagg
      781 cccggtgacg gagatcccgg tggtgagcgt ggcgggcttt gccgtgggag atcacatcct
      841 cgtccgcgcg gaccccggcg atgcctgggc cacggagcat ctggaggatg gctgggtggg
      901 ccacgcgggc agctacgggc ggctgatgta cctccgccag atcatggcgc tggatgcggt
      961 gaacaacgtc atcacgctcg acattcccac ccgctacacg ctcaagacac gggacaatgc
     1021 gcggatttac aaaaagacca cgctgatcac cgaggtggga ctggagggct tctccgtcgg
     1081 caatgtgcag catccgggga cgacgggctg gggagaggaa gactacgcca cggcgggcac
     1141 ctccgcctac gacgtgcata acagctacgt catccgcatg agccgcgcac gcgacggatg
     1201 gatcaagaac gtgcatactt accagccggc gggcaatacg acgacgtgcc atcttttgaa
     1261 caacggcatc ctgctctctg aatgctcccg cgtgacggtg aagggctgct acttccgcag
     1321 gccgcagttc gggggtggcg gcggagccgg ctacatgtac cgcctccaga acagcggcga
     1381 ctgcctggtg cgggattcca ccgccgagtt ttgccgccac ggatttgttt tttcgcacat
     1441 gtccaccagc ggcaatgtgc tgcacgcctg cctggacaaa accacgggaa agcagaccgg
     1501 gaacacgggc aatcaaaaca cctccggcaa aggcagcgat catcacatgc acttcagcca
     1561 ctcgaacctg gtggatgcct gcacggcgga cggcagttgg tttgaggcgc gctaccggcc
     1621 atttggctcg gtgccgctgc acaatctgac cgcggcgcac accgtctatt ggaacacgga
     1681 gggcaagggc acggcggcgg cttatgtggt gcattcccag cagagccgct tcggctatgt
     1741 gatcggcaca cgcggcacgc tgacggcggt gaacaccggc ggcacctcga cgaccaaaac
     1801 gactcccgtg gatcatgtgg aaggcacggc gcagggggac acgctcacac ccttctccct
     1861 cttccgcgag cagcgccgcc ggcggatcgg cctgcccacg gtggccgaga tggcggagac
     1921 acaggcgctt tttccaaaca acaccgcgct ggtgacaccg caggtttggt tcggcgattc
     1981 caccacggcg ccgccagacg cggccttcac ctgggcacgc accagcggac ccggcgctgc
     2041 cgatctgcaa agcgcgggcg gctttgccgt gaatgccgcc tttctcctgc cgggggcgca
     2101 caccctgacg ctcaccgtca gccgccacgg cagcctggag gaggactggg ctgccagcgc
     2161 ccccgccacg gtgcgtgtgc tgcccgcagg cacggtggaa acggagttgc cacccgtcgc
     2221 ggacgcccat gtgcaggaag gcaccgcgga tgcgaactac aacaccaccg tcctatggat
     2281 gaaatatgtc ggcagcagca acccggtgaa ccgggagatc ttcatgcgct ttgatctttc
     2341 cagcctcgca ggccgcacgg tgcagtccgc ggaattgcgc cttaattcca ccgagccgga
     2401 cacggcggcc acgggtcaga cgcatttcgt ggcaaacgac acctggagcg aggcgggcat
     2461 cacctggaac acccgcccgg cggtctcgac tctgctgcaa acgtggccgc tcgccaccga
     2521 ctacgcgcag cgaattgacc tcaccgcggg cactactgcc gaggcggcag gggatggcct
     2581 gctttcactg cgtcattcca tccaaagcca gacaaacagc gccaccgtct tcaaatacgc
     2641 ctcacgcgag cacgccacca cggccctccg tccgcgcctg cgcgtgctgc acagcgaggt
     2701 gtggccttcc ttcactgcgt ggatcagcgg cttcccgcaa atacccgctg aattccgtga
     2761 cgccgatgat gatcctgacg gcgacggcgt gaagaatctg gaagaatacg caagacgcgc
     2821 ccagccgcat ttggccgagg ctttgcccgc aggtctcctg gccgctcggg aaggcggagc
     2881 gctgcgtctt cgcgtgccgg gcggagcatc cgttccgccg ggagtttatc ccgtactgga
     2941 gcacaatgca gggttgtccc cggcgggctg gcaggcgctg atgcgcgtta gtgtggaggt
     3001 gtcgggcgcg gaccttgttt tcacggtgcc gtccgggctt gcgcaggcgg ggccgggctt
     3061 ttttcgtttg aggtacgagg ccgtcccata agcgaccgcg aatcgcctct gccgcgccgc
     3121 aacgtcccgt cgtgtcgggc cgctgaatgc ttgccagcca gtgaaagccg tctaggctgc
     3181 gcgcccctat ggcccaaaaa ctcaacatcc tcgtcgcaga ccccatctcg gaaacaggca
     3241 tcgaactcct caaaaaggag ccgtccttct ccgtggaggt gaacctgggc ttgaaggcgg
     3301 aggccgactt cgccgaggcg gcgaaaaacg cgcacgccat catcgtgcgc agcggtgtga
     3361 aagtcaccgc cagggtcatc gaggccgcgc cgaacctcaa ggccgtgggc cgcgccggcg
     3421 tgggtgtgga caacattgac gtgaccgccg ccagcaagcg cggcgtggtg gtgatgaaca
     3481 cgcccggagg caacaccatc tccaccgccg agcaggcctt caccctcatg atgtccctgg
     3541 cgcgcaagac cccgcaggcg cacgccagca ttgtggccgg caaatgggac cgaaagagct
     3601 tccagggcac cgaggtcaac ggcaagacgc tggccgtcct gggcatgggc cgcatcggcg
     3661 cggagttcgc caagcgcgcc aaggccttca acatgcgcgt ggtggcctac gacccctacc
     3721 tctcaaaaaa ccgcgccgag agcctcggtg tggagctttt tgaaaacctc gacgaggcgc
     3781 tcggccaggc ggacttcatc accatgcaca tgccgctcac gcctgacacc agacacatgc
     3841 tcaacgcgga gcggctcggc aggctgaaaa agggcgcgcg catcattaat tgcgcgcgcg
     3901 gcggcctcat tgacgacaac gcgctggctc aggcgctcac ggatggcaag gtggcaggcg
     3961 cggccctgga tgtcttcgag gtcgagccgc cgccagctga ctaccctctt ctcaaggctc
     4021 cgaacacagt cttcacgccc cacctcggcg cctccacagg cgaggcccag gaaaacgtcg
     4081 gcatcgagat cgccgaggtc atcgccgcgc acctcctgca cggaaccatc gtcaacgcgg
     4141 tgaacatgcc gaacgtggac cccaagacgc tggccgagaa ggggcacttc ctcaaattcg
     4201 gcgagctgct cggccgcctg ctctcccagt tcgccccggc gcgctcgggt cagatcacca
     4261 tcagttacag cggaaaagtg ggcacgggtg atacatcgct catttcacgc gccatcttga
     4321 aaggctatct tgagcgcccc tgcggcaccg agcaggtgaa ctacattaac gccaccggcg
     4381 tggcggaaaa cctcggcatc cgcttcaacg aaagccgcgt gccggatgcc agcgagttca
     4441 gcgacctcat tgaagtgcag gccgcgaacg gcgcggagac ggccagcatc gcgggcacat
     4501 tcttcgcggg atcgccgcgc atcgtgaagg tgaacggccg ccacatcgag acgcgaccgg
     4561 agggcactct gctgctcatc gagaacattg accgccccgg catgatcgcc gcctacagcg
     4621 ccgcgctggg caaaaaccag gtcaacatcg ccgacatgtc gctgagccgg aacaaggagg
     4681 gcggcaccgc cttgacgctc ctcactctcg actccactcc ctcgcctgag gtcatcgccg
     4741 aactggaggc catccccggc atcagccgcg tgcattgcgt ggtggtgtga gccttccggc
     4801 cgtcactgac tcacacgccc aagcagatca aggatcttcg cggtgatttt caccgacgcc
     4861 tcgcgctcga ctcggttggt gcccagccag gtctcgtagc cgccaagctc gtgctgcgcg
     4921 ggcgtgggca ggtagccgtt gcttccattc gccagctcga tggtgagggt gtctttgaac
     4981 gggctgcgag ccttgatctc cagtccgatc tcggtgaaga cctcgaaggg aatcgcggcg
     5041 atgccgagat cgccgacgcg gaaagcctgc atgacgcagc taaccgtgtc cgccttcgag
     5101 tcgcgcgcgg ccagcacgcg ctcggcgtaa ggctgctcca gtctgtgcgc gggctcggca
     5161 tccctgggtt tggcgagcac gcctttcgcc cactcgatca tctccggtgc gggacggcgc
     5221 gcggcgagtt ccaagtcctc cacggcggcc ttcagtggaa cccagtcctg atgtttcaac
     5281 gcaccgcgca cgcggagaac ctcggcggcg agatcgcccg cgacgagctt gatcttctca
     5341 tagggcgcgc gctggacatc gggagttttg ccactgtagt cattgttgtt cacatcgccg
     5401 caggggccgt tggccagaat gccgacgacg ggcgggtgcg cgtcatccga gccgagttcc
     5461 ttttcaatga cgcggcagaa ttcgccgaag taatccgccg acaaatgctc gcgcggcacg
     5521 ccgccgacat agtgcagcca gtagttggcc atgatcgcga tctggcgtcc gtcagccgcc
     5581 tgcacggaga ggcaataaac ctcgggattg acggggccgg cgggaccggc catgcggggg
     5641 cgcagcaggc tggcgggatt catcaccgcg cggtcctggc cgccgaaggg gttcgtgttg
     5701 gtcacgccct ctttgagcag ccagcgcctg ttgaaaacat gctgcggcac cgcgcccgcg
     5761 ccccaggcga tcctcgccgg ctccaagttg ttcaccgcgc ggcgcacgcc atccacgacg
     5821 cgggagatga tgaggctgcg atagttcaac gatgagtcgg gatccgtgat cgcggagacg
     5881 ctggagtgcg tgtgcgtgcc ggagaacatc atgtgcgcgg ccggtatgcc ggttgcggcc
     5941 tcgatttttg acttggcctc gtcccagaca tcgcgcgcca gcgagatggt gtcggccacg
     6001 accaaggcca gtcttgtcga gccgtcatcc agcaccagac agcgcgcgtg cagctcgtca
     6061 tgcacatgcg cggcgattgg cctgggcgcg aaattgccga cgatctccat cccaaggggc
     6121 ggcgtgatgt tgctggtggc cgcgcccgct ttgaagaccc gctccgcgct ttcctgcgcc
     6181 ggtgcgttga cgggaggcag gcagagaatg ccggcgagga tgaaaagagg agcggtgcgt
     6241 ttcatggggc aggagcatac gcgcctggga ccgtgttttt cgtcgagcct tgcaaagccc
     6301 catctgttaa agcgcgggaa tgataacgga gcagtttgga tgaaggaaag gagtgctaaa
     6361 gcgcgatagg atgccgccga ctcatgagag ccatgccctt tcctgccgcc acgcgttttt
     6421 atgccagaat gacccgcgtt tgcgcggcag tggtgatgct gccgctgctt tgcgcacgtt
     6481 ttccggcctt cgccgggccg gagacggacg gcaaggatgc cttcactttg gaaatcaggg
     6541 gcaagtccat cgaggtcttc acctatcgcc cgaagaattt cgacggcggg cgtttgatcg
     6601 tggtcatgca cggcatgaac cggaacgcgc gtgactaccg cgacaacgcg gtgacgctgg
     6661 gagacaggtt cggggcgctc atcgcagcgc ctgcttttga cctggggcag tttcccgtgg
     6721 aggcctacca gcgcggcgga gtcaccaggg acagcaagcc gcagccggag tcggagtgga
     6781 cgtttcgtta cgtctgggac gtggtcggcg agatgcgcag gcgcgaggac cgccccggca
     6841 tggagtgccg tctcatcggg cactcggcgg gcgggcagtt cctcacccgg cttgcaggtt
     6901 tcatgccggg cgacgcggcg cgcatcatcg ccggcaatcc gggaaacctg cttttcccca
     6961 cgcatgacat gccgttccaa tatggcttcg gcggcttgcc agaggctctc gcaggcgatg
     7021 atgcgctcaa gagttatctg gctgctccgc taacgcttta tctgggcttg gatgacacgg
     7081 gcacggcgaa tctggacatg accgaaaacg ccatgcggca gggcgccacg cgcatcgagc
     7141 gcggacgcgc ctgcttccgc atgggggagg cgctggcgcg ggaaaaaggc tggcccttcg
     7201 gctggcgcct ggtggaggcg cccggtgtgg cgcatgactc gaaggcgctc tttgcccacc
     7261 cccaggctgc tgaggccctc tttggcggct ccagtggcgc gctggagcct cattgaagca
     7321 gcgtcacgat ctgtgtctgc gcgatgaaaa tccgccatct ccgcgggttc atcgaaaacg
     7381 atccggcgac atcaaatcca acggcacggg cggttcctcc ccggcgagca aacccgccac
     7441 gatcatgcct gtggcggggg cgaggctcag gcccatcatg gcgtggccgg tggcgactgt
     7501 cagattggcc catgcccgcg cgcggccgat gtaaggcatg ccgtcgggtg acacggggcg
     7561 caatccgctc cagggagtga cggatgaaaa gtcgtcctca tgaaaagccg ggtagtagcg
     7621 tgtgaactct cggatgatgc cgcgcacgcg ccggcgggtg atggattcat ccactcctgc
     7681 aagctccatc gtgccgccca cgcgcagggc gccgtccatc ggcgtcacgg caaggcgcgc
     7741 ctccgtgcaa atggcgcaga ggcttgggag ctggcgcggc ttttcgagcg tcaggctgta
     7801 gcctttgccc gcctgcatgg gaatacggag tccaaactcg cgcgcacagt ccgctgacca
     7861 tacgccggca gccatgacca gctcgtccac aggcagctca tcattcccgc agaaaacgcg
     7921 gaagatttcc cgccgcccac cagccagcgc gcgcgcgctc cagccggtga tctccgtgtc
     7981 gaagcggaag ttcacgccga ggaattcaag ctccttctcc agcgccgcga tgaaacggcc
     8041 cggagccagg tggcagtcga gtggaaaatg cacgctgccc gccacatcca gcgtcaacgc
     8101 cggatcgagc gtggcggtgg ctttggcatc aagtacttgc gcgggcatgc cgagcgcgcg
     8161 gcttttttcg gcgaaacgcg cctcatcctc cagggcgcgc ggctccttgc atagcatgag
     8221 caggccgcgc ttcaccagtc cggtgtcgat cccgcattcc tcgaagcagc gccggctcag
     8281 aaggccgaga tcacgcagca ccggggctgc ggcatcggcg cgcttttgag tggaggcccg
     8341 ccaaaaacgc agcccccacg ccagcaggga gggatcgggc cttggcttga tgtaaaacgg
     8401 cgactccggg ttcagcatcc acttcaaacc aagccgcatc atgccgggcg cggccagcgg
     8461 gatgaaatgg ctgggcacga ccatgccggc attgccgaag gagcatccat cgcgcgcgcg
     8521 aggttttttc tcgatgacgg tcacgctcat gccgcgccgc gcgcaataga gcgcggtgca
     8581 aaggccgacg acgccggctc ccaggatgac gacacgggtg gacatgcctg caaggtgccc
     8641 gcggcgcggc gcgtgtcttg cgcaaagacc gcgccgcgcg ccggaattcg cacaagctgg
     8701 cgcttagcgc acgcgccaga ttttcacgtc atcaatccac acggtgctgc ccgccaaaaa
     8761 actgagccag cgcttcaccg gatgcgcgaa gccgggggag ctgtgcgcgg ccagcagggc
     8821 gccatccagg ctgagccgca gttcatcacc ctcggtgacg agcatcagtt cgtgccactc
     8881 gttgtccgcc ttccacggta cgacgacctg cttggatttg agcagggcct caagatcggc
     8941 ggggagcttt tccttccggt ccaagacatc ctggcggcgc gcgcggatgc cgaggtccat
     9001 cacgccggtt ttttgctcaa tgagcgtgac cgagctttga ctcaggatgc cgtagcagag
     9061 atgccccgca tgcacgcccg gggtctcgcg gtccacaaag cccaactgca aagtctcgcc
     9121 ctggttgatg ccgggaaagc ggaagcgcac cacgactccc ccgtcctgga aacccgccgc
     9181 atgatggatg tgcgcggcgt ggccggcttc cttcgtcgcg ctggcgattt tgaggatgcc
     9241 ctcgtcgagg tcggcctgtt tgaggagcgg cacgcggtcc gccgtggcgc tgttccagcc
     9301 gttgccgatg gcctttgcca ggtttccgtc ctcctgccgc tcaaaggcgt cgtggaaaat
     9361 aagagcgccg ttttcgctca gccacgcggc ttcatcgcgc gcctgaagat cggtgagact
     9421 gagagcgcag agggcgatga caaagtgtgg aagcaggttc atggggggct tgaagaacga
     9481 ggttcgttgt cggcttttgg cagcctttgc tcatgcggac ccggcgcgcg tatggccgtc
     9541 ttttttgccg tgccgatcat acgaaactgg acgatccacg cgcggcggct agtctcgcgc
     9601 gcatggaatc caactggaaa ggaaaaatcg tgatcgtcac cggtggtggc ggcggcatgg
     9661 gacaggcggc ggcgcagcgc ttcgccggag cgggcgcgcg gacatttgtc tttgggcgca
     9721 cgctggagtc tttgcaggcg gcggcggcga tttccccaga gatcatccct gtggtctgtg
     9781 atgtcgcgga cacggccagc atcgcatcgg ccatgcgggt ggtgctggcg gagggcgcgc
     9841 ccttcgcgct cattcacacc gcgggcatca acacgccgga ccgcttcctg gatcatgccg
     9901 acccgggcag gctggtgtcc gaggagagct ggaggagggt gatggagatc aacgtgctcg
     9961 gcgtggcaaa catgatccag gccgtgactc agccgatggc ggccgcgggc ggcgggcgca
    10021 tcgtggtcgt gtcatccacg gcgggccacg gcttcgactc ctacgcggga gtgccctaca
    10081 ccgcgagcaa atgggccgtg catggcatgt tgttcaccgc gcgcatgcag ctcaacgcgc
    10141 gcggcatcgt cgtctccgag tatgcccccg gagaagcgga tactccgctg gtgaaaaaac
    10201 gcccggtgca gccggcggcg gagcacaaga gcgccatgat cacctgcgcc gactgcgctg
    10261 acgcgctgtt tttcatcgcc agccagccgc acagcatgag catcatccag ctccccgcct
    10321 accagccttt tggcggcatg ccgccccaga tcaccgcgcc ctggctggcg ggcctggaca
    10381 tcaagcctgc gggaacgtga gcgggatggt gtgttcaggc tgctcttgaa aagggcgctt
    10441 cagtaccaaa cggaaagcgc gccatgcttg agcatgacac gcccgcgtgt gtgatatcct
    10501 tgccagccgg tgatcgaagg gcaccgctct gctcactgaa attcgaggca gacaccctcg
    10561 gtcgtgctgc gggcaaacag acggttgtat gccagtacgg ggtaactcca cactttgcct
    10621 tcaagcactt cctcgcgttt caactcagtg tatttccttg ggttggcttc gacgacgaca
    10681 agctcgcctt tgtcactaag ggcgatgatg ctgtctcccg ccaggatgac ctggccagga
    10741 ccaaagccag gggtggacca tttcatgtcg ccagtgcgaa tgtccacgca agcgagggga
    10801 ccctcaccgt attttttgaa gctgaacatg ccgtagaggt ggccgtcttt gactacagga
    10861 gtgctccaat ggttggcaaa gtcatcgttt tccttgcgcc agatttcctt ggatttccaa
    10921 ctggagccgg acttgctgat ctggtaggcg cccgcgccca caccgtatcc ggccgagcaa
    10981 taaacgatgt cctcaaagac gaccgggctg gcggcggtgg agatattgaa acgataaggc
    11041 tggcgccaga gcaccttgcc gctttcgggc agcacggaga cgaggccggt ctgggtgaag
    11101 aagatgcatt ggcgtgtgcc gagcagatcg gccacgacgg gcgtggcgtg ggtcattttg
    11161 tcgtcctcac ccttccacag gaccttgccg ctggttttgt cgaggccgag cagcgcctgg
    11221 ccctcgccgc caccacacat catgagcaca ttgccctcga tgacgggcga ggcggcgttt
    11281 tgccagcgga tttgcacgcc ggcgttttcc ttcagcacgt cgcggttcca gaccagatcg
    11341 ccgccggcgg cgctgaagca atggacgccg aggctggagt caatgacata gactttgtcg
    11401 ccatccacga caggggtgga gcgcgggccg tcgccgcctt tgttgtcatt ggtgccggca
    11461 tcgccgccgc cgtcgtattt gccgatgatg gagaggggct tggaccacag ctccttgccg
    11521 gtcctcacat ccaggcagac gatcatctcg ccgctgttgc catcatactc gccggtgacg
    11581 agtgtgaaag ccttgccgcc ggccacggcg aatgaactga agcccttggg agtctcggct
    11641 ttccacaggg ttttgacacg agcgcctttc cagttggaga cttgcggcgc tggaacgctg
    11701 ccgtcattgt tcgggccgcg gaatttgccc cactcaaccg gggcgctggt ttgggcggaa
    11761 agaccggccg ctgcgagcag cgagccgccg aggaggcagt gaagggggca gttcatgagg
    11821 acaggatcag ggtatgggat tgcgggctga gtcaacgccg cgcggcgcgc agtcttgcgt
    11881 ccgcgcaccg cgtggcaagc gaagacttgg gggattggaa gacgagactt agacaagcaa
    11941 ctcagcaatc tgcaccgcgt tgagcgccgc gcctttgagg agctggtcgc cgacgaccca
    12001 gaaagcgaga ccgttgtcga gcgcgcagtc caggcgcagg cggccgacgg cgcagttgtc
    12061 gcggccggcg atgtcgaggg ccagcggata ctgcttggcg gcgggatcgt ccatgacatc
    12121 gaggccgggc gcttttgcca gaacggcgcg cgcggcctcg acagtgacgg gtttttcgaa
    12181 ttcggcgctg atggcgatgc tgtgggcgcg gaagacgggg atgcgcacgc atgtgacgga
    12241 ggcgcggaag tcagggtggt gcatgatctt gcgcccctcg ttctccattt tcatctcctc
    12301 tttcgtgtag ccgctgtcga ggaagctgtc cacatgcggg atggcgttga aggcgatctg
    12361 gtgcggatag acgtgcggct gggaggcgag cttcggcgcg tcccacgcgc ggttttcagc
    12421 ggcccactca cgggtctggc gggcgagttc ctcgatggcc tgcgcgccgg tgccggagac
    12481 ggcctggtag ctgctggcga agatccgcct cacgccaaag gcctggtgca gcggatgcag
    12541 cgccatgagc gagatggcgg tggtgcagtt cgggttggcg atgatgccct ggtggctttt
    12601 cgcgtcgccg ccattgatct cgggcaccac gagcggcacg gcggggtcca tgcggaaggc
    12661 ggaggagttg tccacgacca cggcgcccgc cttcacggcg tgcggggcga attccttgga
    12721 aatgccgcct cctgcgctga agagggcgat gtccaccccg gcgaagcttt cgggggtgag
    12781 ttcctcgatg gtcacatcct ctcccttgaa cttcagcttt tttccggcgc tgcgggcgct
    12841 ggcgaggagg gaaagccggc cgacggggaa agcccgctgc tccaggcagc gcagcatctc
    12901 cgctcccacc gctccggtgg cgccgacgac cgcgacgtgt ttgaggttgc tcatatgttt
    12961 ggggttggtc tgaaaaaggg cgcgcagact agtgccggga gccgggattg caaccgtgcg
    13021 catggaatac catgctccct tcccgcgagg tatccttgcg ggaaaataaa cccggtatga
    13081 tgaagctgtc aggccggttg gtgctcagac gatttgcggc agcttccaat ctccgcggaa
    13141 cggacgcgct gccatcaggg cgtttgcctc ggaatccgcc tcaaaatctt ccgatgccgg
    13201 attccacgcg agcttgcgcc cggctttcaa agcgacagcc gcgagctggc agagagtgtc
    13261 agagcggaca gcggtctcga tgtcgcagac aggctttttg ccggatttaa cagcatccac
    13321 gaaattgcgc gtgtgttcga ggctgaccgg cagcttcacg ggcatggtgc cggctttgct
    13381 ctgcggatcg cgcaaaatgg cgtcgtcgct ggcgctgatc tggccgcgcg tgacatgcac
    13441 ccagccgttc tcgccgatga accgcgcgcc catttgcagg cccatgctgc cgatgttcgc
    13501 gaaggtgatc ggcgcggcct tggcgaattc aaaacgcatc ttccaggtca gcaccgcgtc
    13561 gcagccgcca ttgtcgggat gcgttccctc gccttcgacg ctgaccgggc cggtgtcgtc
    13621 cgtgccattg ccccactgcg cgatgtcgag gtgatgaatg ccccagcacg agatcatgcc
    13681 gagggagaag ttcgtgatgt tctcgtgatt gtctcgcttg agcttctcgc cgctgaacgg
    13741 cgtcatcggt gcggggccga cccagcgctc ccagtccaca tgggcggggg ttttctcctc
    13801 gggaaaggcc ggcatcgtct tgccgccggg cacgccgacc tggatttcgc gcagcttgcc
    13861 gaggcgtccg ttcagcgcga gctcggcggc ccagcgaaat ttcaaatcgc tgcgctgctg
    13921 cgtgccgaac tggaacacga catttttctt ccgcactgcc gcgcgcacga gcttcgattc
    13981 ctcgatggac atgcccatcg gcttttcatg gtagatgtgc ttgccgtgca agaccgcatc
    14041 cgccgacgga atgctgtgcc agtgaaccgg caacgccatg agcaccgcgt cgatggaggc
    14101 gtcgcgattc agctcgcgga agtcggcgaa gctcttcacg tccgcgctgc cgtagcgctc
    14161 cgcgatgtgg cctttggcgc gctcgaggtt ctgcgtgttc acgtcgcaca acgcggtgac
    14221 gcgcacatcg tcgtggttga ggaaggcacg catatccacg attccctgcg cgcccgtgcc
    14281 gatgacgccg agcgtgatct tgttcgacgg cgcggtctgc gagcgcagca ggcgcgcagg
    14341 cacaaacatc ggcgcgacgc cggtggcgag gatctgagtg aggaaggagc ggcgggtttt
    14401 catgtttgtt tatttaagtg acagaataaa ggctttcaca tccgcaaact cttgcggctt
    14461 caagatcaag ttcagcggcg gcatcgggga aatgggcgtg gcggtgtagc cttcggcgag
    14521 cttggcgttg gggttcacga gagactcgat gatgtaggct tcgtccttct tgcttgcgat
    14581 gccgtcgagc gcggggccga cggggctgcc ctggcctttg agcaggtggc agtttttgca
    14641 ggcggccacg gggtgctccc agaagatctt ttcgccgcgt tgaatgtcgc cgatgagctt
    14701 ggaggcgtcg tcttcgtttt cgaggacgag ttcgcagagc ttcacgtcgt cgaaatagac
    14761 gtcgcctttg ccgacgtgga ggaggttgat gctggccttc gggtggttcc tgctgttgaa
    14821 gacgacctcc acctccgtcc agtcctgtgt gcggttgatc tttggcgagg tctcggcgcg
    14881 gccgatgtga tcgttgaggc tggccttgcc acggaagccg tgggttttga tccaagcgct
    14941 gagcctgtag tcggtgttcg gcttcaaagg cacgtcggca aagaaactcg tgtccgcgtc
    15001 gtcacgcgtg atgcaacgca tggcctgctt gccgctgtgg accattttcg cgtcggtgac
    15061 gatgccccac tccgcgcctt tgttgcccgg acgattgccg taatcgcgct ttttccaagt
    15121 ctgcgcgatg ggttcttcaa atccggcgtt cgggagcaga ttcgggcctt cctggtagtt
    15181 cagcaccttg tgcttcttcc cgagcatctt cgcggcctcg gcgagccatt cgtcgctttt
    15241 gacaacgggg tcggcggcga gctttttgac gagcgtttgg atttctggcg tcgtcgggaa
    15301 gtccgcgagc ttcacgaacg cggcgaggcg cgtggtcaaa tcgggatcgg agatcacgcc
    15361 ggagccgaag aagaggtctt gagcggcttt gtcacctccg agagcacgga tggcgttgcg
    15421 gcggagtttg gcgtctttgg agatgagggc ggcttgatgg gttttggcat cgagtttacc
    15481 tagaccgtga agagtccaaa gcgagtgaat cgagccaatt ttaccttcgg ttgctgaaac
    15541 ggcagctttg agatctggaa ctaattcttc ttttcctgaa tgaatgaatg cttgttcttc
    15601 tacaaggacc ttctgaacca aaagacgacc atgctgagta ttttggttca gctctgaaac
    15661 ccagttttta ctggtcgtct tgggagccgg cattgggccg ccaacgtacc ttggtgcgtt
    15721 ttttgccacg atgcggtaga tgcgcccgcg gctgtgatct cgcaggtcat tctcatgcgc
    15781 accgccgaca cccgtggtcg ctacgaagcc gccgcgttct ttgctgggcg tgggattgtg
    15841 ctgaatgatg aaattttgga aatcggccac ccacaccgcg ccatcggggc cgacctcggc
    15901 atgcacgggg ctgttccact cgtcgctgct ggcgtagagg ttgaaggcgt cgagcgcctt
    15961 gtagccagca ccgtcgggct gcacgtccat gagggacacg accttcatcg tcggctcgca
    16021 gaccatggcc atgccctgaa agcgcttcgg cagcgcgtta ctgtaaatga agttcgagcc
    16081 agcggcggag gtgtagccgc cgaagacgtc cacctgacgg aagttcggcg tgatggtgtg
    16141 gcaggtatcg acgacgttga ttttcttcgc cgtcatgccg cggctgcctt cttcatacgc
    16201 ggtggcgggg atgccgccga agaaaagcgg cgcgccattg gccgtgccgc cgaattgatc
    16261 gcccgcgtcg ttggtgctgt ggccccaggc gttgttggtg aactgatgca ggaactcgat
    16321 ggcgctgccg tccggcttga agcgatacgt tcccatcgag aactgctgtt ttttgccgcc
    16381 gacatagccc tcgatgccgc tgtagccgac gcagccgtag atccagttgt cgaagccgta
    16441 atgcaggctg ctggcctgcg cgtgcgtgtc cttgatgccc cagcagtcga tgatcgtctc
    16501 gcgcttgtcg gccttgtcat cgccatcggt gtcttccaaa tagatgaact tcggcggcgc
    16561 ggccacgatg atgcctttgc gggcgaaaac gatgccggtg ggcagattga gcttgtcggc
    16621 gaagacggtg aatttgtccg ccttgccatc gccatcagtg tcctcgcaga ttttcacgct
    16681 gtcctggccc tcgccgcttt cttgaacgcc gtgcgggtag tcgcgcgtct cgacgaccca
    16741 gcagcgtccg cgctcatccc aagcgaacgc gatgggcttc gcgatgtccg gctcggccgc
    16801 gaatagcttc aactcgcaat ccgccggcac ctgcgtgcgc tccatgctgc ctttgggtga
    16861 aaacggcttc tgaaaggtca gcggctccgg gcgcttctcg taattggcca cttgggggtg
    16921 tttctcgcgt ttctcaggct ccctttgggc aaaaaaagcc tcgtaggcgg ttttgcgctc
    16981 gggggagagc tttccaagaa ttcgagcctt gaggtcggac gggtcggaca agtcggacgg
    17041 cgtcagcgtc agatcgtaag cggcatcgat cttctctttg gcgtagtcga agtagatcgc
    17101 gtcacggccg aggtcacgca tggcgttgtg cagcggagca gtatcggcac cgggcgagtc
    17161 gatgtagagc acgcggatgg gacctgccat ggcggaagag gcggcgagca gaaaggtcag
    17221 gcatgaaacg gtcatgtgga tggctggaag aatgcgccat accaacgccg cgataccgat
    17281 ggtcgcgcgg acattccttg tgtgaaatcg gacactggcc cttgaagggc gcgtctgttt
    17341 ctgacagttt gcggcatgag cgggccagca tccagccgag aggccttttt ccggagcatg
    17401 gcgccggaga gccagtttta tcttttgttc aaccacctgc caagggtgtc cttttttgcc
    17461 aaagacgcgc agttccgctt cgtggcggcg aactgccact ttgtggagcg tctcggcttt
    17521 tcctgcgagg ctgagctttt gggcaaggga gactttgatg tcttcccgtc gcgtctggcc
    17581 gagaacttcc gccgcgatga tgaggaggtg ctgcgcagcg gcgagccgaa gctgggcatc
    17641 gtggagctgt tcttcaaccg ccagggcatc cccgactggt ttgtgacaca caagctgccg
    17701 gtcctgaacc gcaaaggcag cgtcatgggc atcatgggca tcgtgcgcgc gcacggcagc
    17761 gcgccggacg acgcgcatcc gcaccgccag atcgagcgcg cggtggagca cattcgcacg
    17821 cgcttccggg aaaagatcac agtgcctgaa cttgcggaga tggcggcgct ttcgccgcgg
    17881 cagttgaacc gcaagtttgt cgaggctttt ggcatgagtc cgcaggccgt ggtgatgaaa
    17941 gtgcgcatcc aggcggcgtg cgagctgctg gcgtatccgg acaagccggt gggcgaggtg
    18001 gcgcgcgagg tgggtttcgc cgaccagagc tccttcaccc agcagttcca ccgccacatg
    18061 ggccagacgc cgctgagcta ccggcggcag tttttgtgat aaaaaaatcc ccggccctcg
    18121 acttggagga ccggggatga gtttagtgag gccagcctca gactttccag ccggggcggt
    18181 aggccgggcg ggtgacgaac tggttggcgg actcggcgtt gggcactttc atgccttcct
    18241 tgtcccactg cacggtctgc tgggcgcgca cagccaggtt gccaagcagg acgaactccg
    18301 tcagcgggca gctgtagtcg aagttcgagc ttgggctgcc gccgttgacg atgtgccagg
    18361 cccactcgcc ctgcggattg ttcggaagca cgctgcgcgc ctcggttttc ttgatcttct
    18421 cagccttcat gtcgaggcgc acctgggtgt agcgctcctc gttgtagatg accgggctgg
    18481 agcagtaggc ctcgccttcg aagacggtgg cctcggtgcc gatgaacatc atgccgctgc
    18541 tggccttttc gaaggccttc ttcgcgaggt cgctggtcat gtgcggggag atttccggct
    18601 tgttcggcat gccgtccttg gacccgtcgt tccaggaaac cttgagcgcg ggatgcttgc
    18661 cggccggcat gtgatagacg aggtcggacc agaccggagc gcaggtcgtg gagatggggc
    18721 tgctgacagc gtcaattttt tcagggatga gctggccgag cacgctgaag gtggcgtcca
    18781 tgatgtggca gcccatgtca cccagggcgc cggaaccgaa ctgccaccag ccgcgccacg
    18841 cgaaggggtg gatgctgttg ccgctgcgtt tgttggtcac gccttcgggc aggacgaact
    18901 cgaagtagga ttcgtccgcc tcggaggaga gccagaggtc ccagtccagg tttggcgggc
    18961 actcggcggc ttttttcgcc agcggtccct gcggccagat ggggcggttg gtccagaggc
    19021 ggatttcttt caaggtgccg atggcaccct gctcgatcca ttccttggcg aggcgctggc
    19081 cttccatggt gcggccctgg ttgcccatct gggtgaccac tcccgcctgc ctggcggcct
    19141 ggtgcagctc gcgcacctca ttgatgtaat tgcacagcgg cttctgcacg cagacatgct
    19201 tcttgagctt gatggcgtgc atggcggccg tgaaatgcgt gtggtccggc gtggagacga
    19261 tgacggcgtc aatttcgtta gccatctcgt cgaacatctt gcggtagtcc tgatagagct
    19321 tgggcgcgcg cggctcgctc ttgccggatt tgagatggtg gtcggcgttc tttttgccgg
    19381 cttcctccag gcgcttcatg tccacgtcca cgagggccac gatgttgtgg tcgagcgtga
    19441 cagcttgtgt gtctgactgg cccttgccgt tggcgccgat gatggcgatg ttcaagcgtc
    19501 tgcccgcggc ctcctggccg cgcaggagga ggttaggccc cgcgaaggcg gaggctgcca
    19561 ggcccgccgt gcggcccagg aaatgacggc gtgagatgat gttcaatgcc atgtgctttg
    19621 gtgttggatg tggtgtgggg aagccggaaa acgcgctgcg ccgccggaaa ttacactgga
    19681 gggtaagcag gaatgagcgc gcgttcaatg cgggaaaacg gcttttcaag cccgaatgga
    19741 ccgccgtcgt gcgttcagtc aaggcggggc gatccgaatc ttcaaacgcg cgccgccatc
    19801 ctgcgcggca tggggcaaaa gatgtggtgc aatatcaaag cctcgctgtg cgggcctgat
    19861 gcgctgctcc ccgggaaacc cggccaggca tcggtccggc tttctcaacg attagcgcct
    19921 catacaaaga gcggagagcg ctgcggggaa ccgtttcaat ccatcccgcc agggagaagg
    19981 tttgggattc cgtcttcgat gcggaacagg cgggtgccat ccttgcgcat gagggctttt
    20041 tctcccggcg gcaacccctc gcaggccagt tcctcactgg cagcccagcg cagttcctgg
    20101 cgggtgtcgg ggcagcgcag aattgggagg atttggttca accagtcaag attcatgggc
    20161 gaagaaaacc gctgctccgc aggcagcgcg gcaacccggc ttgaaaccgg acggcggtgt
    20221 gtcagggagt tttgggagcc gctggctcgg cgggcgcgtc tccggacttg gggactgccg
    20281 gtgcgggtgg ggctgggaca ggcgcggcgg gcttggcttc gtccttcttc ggtgcagcag
    20341 gtgcggaagg tttgtccggc gaaacttcaa ccggtggcgt ggtcgcggat ttcggagcgg
    20401 gggaaggggt cagattgatc ggaggcatgg tggacaaacc actgacgccc ggtgacagga
    20461 gagtgccgga gtcaagcttg atctggggag cgccagcagc gggcgcggct tcctccttgg
    20521 gtttcggggg accgtccact tcggtggtgg ggagtttggc ggcgatccat ttgaggcggg
    20581 tctcgccctg gttgaggatg tcctcgctgg cgttgctgaa cttggcgggg agggattcat
    20641 agactgtttt ggcctcgtcc tccttgccct cggcccagag gatgtcgccc aagcggatct
    20701 gagccacggc ggcgacgtcg gatccgggat tgcctgccac cagtttctca aaggcggcct
    20761 tcgcctcggc ttttttccca atgctgtcga acttcgaagc cagggcgagt tgcgcctggg
    20821 attccagcgg gtggccggag tgcttggcga ggaaggtgtt gagggtgtct atggaggagt
    20881 ccttcttgtt gtcgcgccag agcaggtcgg ccttcatcag gagcgcgttg cccgccgcgt
    20941 cgctgcctgc gcgctcggcg atgacgatgt cgtaatcctc gatcgttttg gcggcggcga
    21001 aggctgctgc tgcctcggca tccctcaccg agccaaagta gcgggccagt ccgtaaatga
    21061 ccacgagggc gatgagcgca aggcaggcgc ggacaatctt tttgaagttg gcctccagaa
    21121 actcctccat gggagtgagc ttttcgagac tatcgggctg ggcggttggg gtggacatgg
    21181 accggggttt gatggggcgg aaagatcagg cgcctacctt ggcgcgggcc tcgtcggcga
    21241 gggcggtgga cagataacgc tcgccggtgg agcaggcgat ggtgacgatg agtttgccgg
    21301 cgttttcggg gcgttgggcc acgcgcaagg cggcgacgac gttggccccc gtgctgatgc
    21361 cgacgagaag gccctcctcg gcggcgagcc ggcgcgccat ggcgaaggcg tcctcattgg
    21421 agaccttgac gcactcgacg atctgcgcgt tgcccgcgct gtccttgagg tggaggtttt
    21481 ttggaacgaa tcccgcgccg gtgccctgga ttttgtgcgg gcctggctgc acaggctcgc
    21541 ctgcgagtgt ctggttgatg accggtgatg cctcaggttc gacggcgatg gcttgaaagg
    21601 agggcttgcg aggtttgatg acctcgacgc agccggtgat ggttccacca gtgccgacag
    21661 cggcgacgag gatgtccaca gagccatcgg tgtccgccca gatttcctcg gcggtggtcc
    21721 tcatgtgtat ggaggggttg gcggggtttt cgaactgctg cggcatccag gcgttcggcg
    21781 tttctttgag gatctcctcg gccctggcga tggcgccttt catgccctgg gggcctggag
    21841 tgagcacgag atcggcgccg agcagcgcca gcagggtgcg gcgctccagg ctcatcgtct
    21901 cgggcatggt gaggatgagc ttgtatccct ttgccgcggc cacaaaggcg agggcgatgc
    21961 ccgtgttgcc gctggtgggc tcgacgatga cggtgtcttt tttcagaatg ccccgctgct
    22021 ccgcgtcctc gatcatggcc atgccgatgc ggtccttgac gctgccgagc gggttaaaaa
    22081 actcgcactt cagggcgatg gtggcgttca cgccttcggt gaccttgttc agcttcacca
    22141 gcggggtctt gcccacggtc tcgacgatgt tgttgtagat catgacggtt tcggttcgcg
    22201 gttgaatggc tctttgtcag agaggggatc aatgctgcgc gcagaactcc tcaaagcggt
    22261 ccatgccggc gttgatcaca tccaggccgg tggcgtagct gaagcggatg gtgttttccg
    22321 cgccgaaggc gacgccgggc acggcggcca ctttggcctt gctgaggagc ttctcgcaga
    22381 agttgacgga gttgatgccc atgggctcga tgttcaccag gaaatagaag gcgccgagcg
    22441 gctcgacgac gcgcacgttg cggatgccct gaaggcggct gagcatgtac tggcggcgca
    22501 cgtcgaactc gtcgcgcatg tcggccacga tctgctgatc gccggagagg gcggcgatgg
    22561 caccgtattg cgcgaaggtg gtcgggttgc tcgtcgtgtg gctttggatg gtgtcaatgg
    22621 cgtcggcgat cttcttcggc gcggcggtgt agccgaggcg ccagccggtc atggcgtagg
    22681 ctttggagaa gccgttgatg gtgatggtga catcgaagat ctccttgctc aggctggcga
    22741 tgctgacgtg cttgttctcg ccatagacga gcttttcata aatttcgtcg gagagaatga
    22801 ggatgtcctc gccggcggcg atctcgccga tggcctgcaa ctcctcgcgg ctgtaaaccg
    22861 agccggtggg gttgccgggg ctgttgagga tgatcatctt ggtcatcggg gacatggcgt
    22921 cctcgaactc gtcggcagtc attttccagc cgttttccgc cttggtctcg acgatgaccg
    22981 ggatgccgcc gacgagcttc accatttcag ggtagctggt ccagtagggg gcggggatga
    23041 tgacctcgtc gccggggttg atggtggcgg cgatggcgtt gtagcaggaa tgcttcgcgc
    23101 cgcagttcac gctgatctgc gaggggtcgt actggaggtt gttgtctatc agcagcttgg
    23161 cggcgacggc ctcgcgcagt tccagcagtc cggcgctctc cgtgtaacgg gtctggccct
    23221 tgttcagcgc ctcgatggcg gccgcggtga tgtgcgccgg ggtgttgaaa tcaggctcgc
    23281 ccgcgccgaa gctcagcacg tccactccct gctttttgag cgccttggcc tggctggtga
    23341 tggacagggt catggaaggc gaaagctcgg cgacgttttt ggcgatgaag tccataggat
    23401 aaggtgcggt ctgagggatc aaaaagtgag gttgcgggaa gggctggcta aaaaggagcg
    23461 gcggcgggct ttgtcaacga cgggccgttg cggcattcag cggccagcgc tccatgtttg
    23521 ccccgcgtcg gccctccatg cggcttgacc ggcccgcctc cccttcccat catggcgcgc
    23581 atggaacgca aaagactcgg acgcagcgga atcgtagtca cggacatctg catgggcacg
    23641 atgaccttcg gcctccaggc cgatgaaaaa acctccttcg aaatcatgga ccgcgctcat
    23701 gacgcgggca ttgattttta tgacgcggcg gaaatgtatc ccgtgccgcc cagcgcggag
    23761 cgtttcggca tcacggagga gatcgtcggc cgttggttga agaccaggga gcgcggccgc
    23821 gtgatcgtgg ccaccaaggt caccggcccc ggccacggct ggttccgccc gcccgtgcgc
    23881 ggcggcttca ccgcgctcga ccgccgccag attcaccgcg cctgcgagga cagcctgcgc
    23941 cggctgcaaa ccgattacat tgacctctac cagacccact ggcctgacca tcacatgcgc
    24001 caggaggaca ctttggaggc gctcaccgaa ctcgtcgagc agggcaaggt gcgcgccatc
    24061 ggctgcagca acgagacctc atggggcctg atgaaaaacc tatgggcctc cgaaaagcac
    24121 ggcctggccc gctttgacag cgtgcagaac aacttctccc tcatcaaccg ccgctgcgag
    24181 agcgagctgg cgcaggtctg ccgcatggag ggcgtgagcc tgctgcccta ctcgcccctc
    24241 ggcggcggcg tgctcaccgg caaatatcaa aacggcgccc tgccgcccgg cgcgcgcttc
    24301 agcgagtatc tggtcagcgg cggcgagcgc cagcagcgca tggcgcggcg cttcgtcaac
    24361 gagcgcaccc tggagaccac ggcgcggctt tccgtcatcg ccgcggacat cggcgtcagc
    24421 gtcaccgcgc tggccgtggc atggagcagg cagcatgatt ttgtggcctc caccatcatc
    24481 ggcgccacct ccgtggccca gcttgagcaa agcctccagg cgcgcgatct catcctcgac
    24541 gcggagaccc tgcgccgcat tgacgagata gacaccgcca tccccaatcc catgaccgag
    24601 gacgggctgc gccggctgtg aggctcgcgc tcacagccgc tcgcgcgcgc cggagcggtc
    24661 ggcgggagag cgggaggtga tgaaggcgct ggcggcgatg atgccgatgc tgcacacgac
    24721 tccgagcgtt tggaagctca tgactccgag cgcggcgaag gcgaggtagg gggcgagcac
    24781 gccgcgcagg ccggtgagaa agctgtggac gctcatgtac tcggccacgg cgtgctccgg
    24841 ggcgagcttc gtgacccaca ggccccaggt gacattgccg cccgcgttgg ccacgcccca
    24901 caaggccatg ccgagcgtcc agcaaaacat gttttccccg agataaaaaa cgaggatgcc
    24961 ggcggcgaag atgaggttga gcactgcgcg gactttgaag aggcccatct tgtcatagat
    25021 gactccccaa ggataggcgc agagcatgcg gaagagcacg ggcaccacgc cggtgatcca
    25081 ggcgacatcc ctccccggca ggttgatgcc gtggaccggg ttggcgaggt attccacaaa
    25141 gaggcaggcg gcgacgagat tgccgatgcc catcatcatc caggagatga gcagggtgcg
    25201 gaagtcgcgg tcctttttca cccagcgcaa ggcggcccag agtccgggcg gcgtgtcctc
    25261 acgcgcgctc cagaccacgg agggcaggcg gcgcgtgaga tggccggaga tgaaggcgca
    25321 ggcggcgaaa atccagagca gccagcggta atgaccaagg tcctgcccga tgagccagcc
    25381 gccgaaaaag ccgaagccca tggcggcggc ggcgcgcaca acaccggcga tggagaagag
    25441 cctgccgcgc gcggcctccg gatagttcag ccgccagatc tgggtgagca gcggtatctg
    25501 catggagaag cagaacagac cgaggctgag accggcgacg aacatccacg gcgaacgggg
    25561 catgagggcc gccagcgcca gaaagacgcc gcccaggaac tgcacacgcg ccgccgtccc
    25621 cgccagcgtc gagcgcgagc gcagcagcag agggacgaca aacagactgg tcatgaggcc
    25681 gccgctcgtg gcgctgagaa agacggactt gcccatgtca tccatggcga agacgcgcac
    25741 ggcgatgagc atgccgaaag tggtctgcag cgtctccagc acgccggatg gcagcgagcg
    25801 ccagacttcg ttgcggaagg tcaggcgcgt tgcgtggttc gtggatgctg tcgagtcagt
    25861 gctgatggtg gatggatgga gcaggagcgg gacgggtcaa tgccacgatg gcggatcgtc
    25921 ctcgaaaccc agcctttcac cgctgagcgg gtgcgtgaag gtgatccgcc atgcatgcag
    25981 gcacatgggt ggcgcttcca cgcccagggt ctgcgtgacg ccaagcgcgc cgccggggag
    26041 atagacgggg tcgccgcaga cagggtggcc aagccgccag agatgaacgc ggatttgatt
    26101 cgtgcgcccg gtgaggggtc tggcctccac aagagaggtg ccatcctcat cacgacgcag
    26161 caggcggaag gcggtgcggg caggcagccc ggctgtctca tccacgtcac ggctgcccag
    26221 ttctccggga gcgccgctga tgggcaggtc acaaagacgc tcctcccagg ccgggtggcc
    26281 cgtgacacgg gcgaggtact ctttttccac ctccccgcgc gcgaactggg tctgcagcag
    26341 tccggcaaaa tgacgggtgc gcgcggcgat gaccaggccg gtggtgttgg catcgagccg
    26401 gtgggcgggg cgcggctttt gcggatgcca ggcggtttgg aggaaatgct ggagcgtgtt
    26461 gcggtggtaa cggccgccgg gatgcatcgg cagcggggcg ggcttgcgca ggacaatgac
    26521 agcctcgtcc tcgaacagga tgcggatgtc cgcactgacc gcgggttcga catcggcggg
    26581 ttgcagccgc aggtagcgct cgcccccctg gacaacgcgc tccggtgagg caggctgccg
    26641 gtcctgggcg agcattctct gattttcaaa aagcatgagc cattccggcc gcgacacgcc
    26701 gggcagcagc aggcagagcg cgtcgagcag gctcatgccg tggcagcgcg cggggatgga
    26761 aatggggcgg tggttgtcct gtggcgtgga gccgggcaga ggatcggcgg cacgtcgcag
    26821 ggcctcctgg cgcgcggcca ggctggcgcg gcggcgctcg tcatcggtct tgaagcaata
    26881 cgggcacgag actccctcgg tgaagcgcgg gtcatcctgg tcccgcgccg tcagcggcgc
    26941 catgcaggcg tggcattgag cggatccggt ctcgctcaag gcggggtcga ggccggtgcg
    27001 ctggtcgaag acgaagcact cgccctcgta atgcgcgccg ccgacctcct cgaagtattt
    27061 gaggatgccg ccgtcgagct gccagacctg ctcgaaaccc tcgcgctcca aaaacggggc
    27121 ggccttttca cagcggatgc cgccggtgca gaaggtgacg acgggctgct tcttcaaccc
    27181 ggctggcagc cggcgcacgg cgtcggggaa atgacggaag tggtcaatgc ccgccggcag
    27241 cgcgcctttg aaggtgccca gcctcacctc gtagtcgttg cgcgtgtcca gcagggtgac
    27301 gggccgccct tcatcgagcc attgtttcaa ctcgcgcgcg gagacgcgcg gcgaggtgtg
    27361 gcgcgcgggg tcaatgccct cgacgccaaa cgcgatgatc tcctttttga tccgcaccag
    27421 catgcggcgg aagggctggc gcgcgctgag gctgtgcttg ggcgccagcc gtgcgagtcc
    27481 gggcatggcg cgcaaaaaat cgagcagggt ttcgatcccg cgcgcgtctc cagccacgaa
    27541 gatgttgatg ccctcggtgc tcagcaggat ggtgcctttc aagccctgcg cgcggcacag
    27601 ctccagtaag cgcggacgca ggctcttcaa atccgccagc ggggcgaaga ggtagcagga
    27661 gatgttggtg acttcggcgg cggcggtcat gacgggctgg atggaaacaa ggatgcgcgc
    27721 gccgcgcaag cgggcattcg cctccgcgcg gccctcatgg ccgccgcccc tcgtccacgt
    27781 ctatgtaatg cgcgatggtc tggatgtcat ctcgcctctc cagaccgccg tcggccttgc
    27841 gcttctccag gatggccgag atgtggtcgt tccagccgag ggtctgcaca tagtggttcc
    27901 agacgagccg gtcggtgtcg ttgagcgggc ggcttctttc ctggcaccat tcgagaattt
    27961 cctcatccga gccgccctgg agcacgcgcg ccttgatgtc ctcgtagtcc acatgcagaa
    28021 agccggccag cgcgccatca ctgcctttgc cgagattcgc gtgcaggtcc tcccacagcc
    28081 tgccctgcgc gtgcaggcgt atcttggccg tcatgcgcgg gaagtatttc agaccggcgg
    28141 tctcatcgag cgggctgcat ggaagcgtgg agggatcagg cttggacatg aggggatgaa
    28201 tggcacatgg aaaagagtct gccaagctcc ggcgcgcctc gcgttgccgt ccgacgccag
    28261 gcccgccagc ggcgacgacg tcggcgagga actgcgccgc ggcggagaca ttgacaggag
    28321 gcttcggtcc cggcagcggg ccgccctgtc cgagcggatc ggtggcgagt ccctggtcgc
    28381 tgatgggagc ctggacgctc agcggatcgg tgatcagccc ggcggaggcg gtggagaacg
    28441 gggcgaggcc cggcacgagg gcgatgagga gggtggattt cgttttcatg ggaggtggcg
    28501 tgtgcggatg agtgtttcga aggcttctcc atcggcaggt tgccgccagc agttgcttca
    28561 tggcggtgat gcacggcttt ccggcgggcg ttacaggcgc ggtaaacttt tttgcctcat
    28621 atccgtcttg acatcagcca gacaatcaaa gtttgattcc gcgaattatt gattatgaaa
    28681 gcatttcttt gccacaactc caacgatcag gcatttgttg accaagttga caaatggctc
    28741 aggcggttcg gattggggac attcaactac acccgcgaac aatgcgcttc caaggaatgg
    28801 ccagcccaaa tgcgtgaaga ggcccggcag tgccccgcca tggttatttt tgtcggtgga
    28861 aagttatccg acccccaaaa acaagagatc accaccgcgt ggaatgagag tgaagtccgc
    28921 gtcaatgccg gaaaatccag gaagcagttt gtatttgtgg cagtcgcggc gaaagcagac
    28981 ttcaagtcag ttcctcatgg aaccgccgat cctaaatttt taccgcgcat tgattatgat
    29041 ccgggaagat catccgagca agttgcccgg aaaatcgcct tttatttcca tgtgcccact
    29101 gtcgtcaatg ggctgccttc gaatccgaga ttgtttaatt atgagaagat gattctcagg
    29161 catcttgaga atgtcgaaaa gcacaaggga gacctgtatt tggaccatca aaagcaggat
    29221 gccaaaacct cgctcttgct tgaggaactg cggcgtctca gtcttgctgg agcgcccctg
    29281 gaatggccgc aagttcgcga gatcaaggag ttcatgaacg gcgcccctgt tccaaatccc
    29341 tgggtcggcg tggaaggaag cacggggttc gatttcggcg tggtcagaac agaaaatcac
    29401 cgtgtgcgca tgtcggccac agttgatggt tccgacggct cgcacgacgc tggaaaatac
    29461 accctgcctg aggcgggacc caggggcaaa ttgtggtttc ccccgaagaa tgccaaaggc
    29521 tttaaagtcg gagtgctggt ttccggcgga atagcacctg gcataaacgc cgtcatagac
    29581 gggattgtgc aaaggcactg gctttatcat accaggaacg aaaacggggc atgcccggtg
    29641 atatacggct accaaaacgg cttcagctct cttcttggcc ccggccgcgc caatcagccc
    29701 tacagactgc tggctgcgtg caaaaagcac aaagatgaat acacgagcca gggcgtccac
    29761 gggcgaagca actcgattat cacttcggag tacgcgaacg aaggtggctc gattattgga
    29821 acatcgagag tggatgaact cacttctccg gactcggtcg agtttcttga ggacatggcg
    29881 cgcacacttt tgaatgatga aattgacgtg ctttatgtga ttgggggtga cggaagcatg
    29941 agagcagcgc acgcactgtg gacggtggcc ttggaaagga tggaaaaaca ggaaggcaaa
    30001 cagagaaaac agaaaatcct gtccatcgtg ggtgtgccca aaacgatgga caacgacatt
    30061 ctctgggtct ggcagtcatt cggtttcatg tccgccgtcg aaaaagcccg cgaggtgatc
    30121 gaaagccttt acaccgaggt ttccgccaat ccacgccttg gggtggtcca gctttttggt
    30181 tcggtttccg ggtttgtggt cagccacgcg gtattggcca gttccacggg tcactgtgaa
    30241 ctggccctga tcccggaggt gccctttagc atcgagggag tggctgagca catgcgcaag
    30301 aaaatggcgg cgaatgccaa cgcaaaaggt catgacatgc ctttcggcct catcgtcatg
    30361 gcagaaacag cgattccgac ggatgttgac aagtatctcg atttgccgga caagccggca
    30421 tcaaaagacg agcctgaccc tcatgcggtt taccgcgtcg agctgaccga tgaggaggaa
    30481 aaagctctca aggactacct gaaaaacaaa aatatcatct ccggtcaaac cagcgattca
    30541 ctgcgcagag ccggcctcaa actcatgagc acgggtctcg caaacctgct gaaaagcacg
    30601 cccgagacga acggattgca atggcagaac ctccgctgtt tgacgaacga accccgtcat
    30661 ctccttcgct cggcggctcc aagctgctcg gacatcatca actgccagag gctgggaact
    30721 ttggcggtgg acaacgcaat gggaggctat acggacttca tgatctccca atggctcacg
    30781 gagttcgtcc tggtgccgct ctccttggtg gtccttggtc gcaagcaaat ctacactcca
    30841 ggaatcttct ggaagtctgt catcgctaaa acgggccagc acgaaagcga catcaatcaa
    30901 gtctcctgac tcgtccgcca gcgcggagcc aggcgttccg caatccatgc aaggtcatgc
    30961 ctggatggtt ccgtggttgt cacgatcctt gcttcttgtg aaagtccgcg ccaataagaa
    31021 tgcggcatct accttccgcg gccccccgcc gcgcatccac atggctgacc acaccctcac
    31081 cctctccaac ggcctcacgc ttggctacgc cgaatacggc gaccccgccg gcgcggtcat
    31141 gttttacttc cacggctggc ccagcgggcg cgtgcagggc gagttgatgc atgaggcggg
    31201 cctcaagttc ggcctgcgca tcatcgctcc cgacaggccg ggcatcggcc tctccgattt
    31261 ccagccgcgg cgcaggctgc tggactggcc gcccgtgctc gaggagctgg ccgcccatgc
    31321 cggcgcggag aaattccacg tcctcggctg gtccggcggc ggaccctacg tgctcgccac
    31381 cgcgctgaaa atgcccgcgc gcctgctcag cgccaccatc gtctgcggcg cgccgccgct
    31441 cgcctttttc ggcgatgcca aaatgttctg gctctaccgc ctcatgatcc gcctccgcgg
    31501 ccacttcccc tccgtgctcg gcgccgtgct gcgcctcggc gcgctcctgg cccggggcag
    31561 cccggacaag ccgccgctca agtggctcat gcgcctgctg ctggagcccg accgccgcgt
    31621 cctcgccgac cccgaagtct tccgcgtcgt ccgcgccggc atgatggaga gcctccgccg
    31681 cggcccccgc gccgtcatcg gcgacgccga catctacctc tccgagtggg gcttcgaagt
    31741 cagctccgtc accttccccg tccacctctg gcacggcaaa atggaccgca acatcgcctg
    31801 ggaatacagc gcccagctcg ccgagttgat gccccatgcc gacgttcact ggctggaaaa
    31861 cgaaggccac tactccctcc ccatcctcca caacgaggcc atcctccgcc acgccctcgg
    31921 ccctggcgcc gccc
//
LOCUS       DAOMYJ010000032        31439 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Prosthecobacter sp. isolate AMR_MDS_5629
            NODE_521_length_31439_cov_23.594093, whole genome shotgun sequence.
ACCESSION   DAOMYJ010000032 DAOMYJ010000000
VERSION     DAOMYJ010000032.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867850
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prosthecobacter sp. (wastewater metagenome)
  ORGANISM  Prosthecobacter sp.
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Verrucomicrobiaceae; Prosthecobacter.
REFERENCE   1  (bases 1 to 31439)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 31439)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.30
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:34:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,742
            CDSs (total)                      :: 4,684
            Genes (coding)                    :: 4,676
            CDSs (with protein)               :: 4,676
            Genes (RNA)                       :: 58
            tRNAs                             :: 55
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31439
                     /organism="Prosthecobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_521_length_31439_cov_23.594093"
                     /isolate="AMR_MDS_5629"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1965333"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1352
                     /locus_tag="PLP58_05400"
     CDS             <1..1352
                     /locus_tag="PLP58_05400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009963832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="sulfite reductase flavoprotein subunit alpha"
                     /protein_id="HRJ07983.1"
                     /translation="ADLVKDKNVLLITSTWGEGDMPDNAVSFWDAINQNGSSPKLDGV
                     KYSVLALGDKNYGDTFCLAGKKLDQRLAELGATRVVDRVDCDVDFDDLAKEWSKNAFT
                     IFGASKPSDVSDMSDPSDKAWSKKNPFPAKLIANDTLNAPGSAKDTHHIAFSLTGSGL
                     TYEVGDALGVFVQNCPEVVDAVIAAHGFDPSAKAGTKTLSEALLTDYEIRSLLGKTPE
                     PGLTLDSFLENLRKLQPRLYSIASSIKAHPDEVHLCVGAVRYTADGIAHKGVASTFLS
                     DRFPLGDTTGIYFHAANHFRLPTDLTKPVIMVGPGTGIAPFRAFLEEREATKAPGKNW
                     LFFGDQKRATDFLYHDQIIAWVQSGHLTRLDTAFSRDQEEKIYVQTRMLQAASELWQW
                     LEEGAHFYVCGDAKRMAKDVDDALHKIIETQGNKTADEAAAYVAHMKKTKRYARDVY"
     gene            1333..3087
                     /locus_tag="PLP58_05405"
     CDS             1333..3087
                     /locus_tag="PLP58_05405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009963833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NirA family protein"
                     /protein_id="HRJ07984.1"
                     /translation="MPAMSIDAPRVASAQVGEDFSNEQKRYLEGFFAGIAAGGVSFGD
                     LSPAPAEPAGPSLDDLTKEERIKRELHPFDAIEQLVLDARWNAKPEPESVFRYKWNGL
                     FWLNPVKDGYMCRLRIPGGVVKSYQLRELAAISRELTSGYIQITTRNNFQIRLIEPKS
                     CPEVLRRIQGCGLHSKGAGADNIRNITMNPCAGYDPHELIDVRPLVNELATLIIGSKE
                     FYDLPRKFNIAYDGGGLISSVEDTNDIGASAVKIIENSESIEPGVYFRIALGGVTGHQ
                     TFASDWGVIVKPEQLNDVMVALLRVFIEFGDRTNRKKARFKYVVEDKGLDGVLVEAEK
                     HLKFKLTRLAPDSPIQAKREFSQQGHSHVGIHPQKQDGLVYVGASVPVGQLTAKQMER
                     IADLADSYGSGEVRLTVWQNFIIPNVKTAYAATLAKSLKKLGFPCEQSNLKSGFVACT
                     GNRYCKYAASDTKGHAIAMMDYLDKRVKLDKPVNIHFTGCAHSCAQHFMGDIGLLGTK
                     VKSESGEGYHITVGGGFGENRKIGRQIFSGVPFESLGATLEAMLKGYLKSRQGEESFH
                     SFCNQRTIGQLQEAFSVA"
     gene            3105..4646
                     /locus_tag="PLP58_05410"
     CDS             3105..4646
                     /locus_tag="PLP58_05410"
                     /EC_number="1.8.5.3"
                     /inference="COORDINATES: protein motif:HMM:NF016838.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0019645 - anaerobic electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dimethyl sulfoxide reductase anchor subunit"
                     /protein_id="HRJ07985.1"
                     /translation="MHDLSDRSDLLHKVLREQQTLRTPVALFAEEYDSKSVRQRFTHL
                     IPLSKPNPGEQYAFQVNLDACTGCKACVAACHSLNGLDEDESWRDIGLLVGTRKQPYL
                     QTVTTACHHCEDPACSNGCPVLAYDKDPVTGIVRHLDDQCIGCSYCILKCPYDVPKFN
                     LKRGIVRKCDMCQGRLAEGEAPACVQACPNEAIKIQVVKLSDVPDRGQIIAGAFDSSY
                     TRPTTTYTSSKSLPEAKPADEGQLRLDHAHTPLTWMLVLTQMSTGTFLINALVVWMGY
                     FTAEDTRVTLLTALTMGVVGIALSVLHLGQPLKAWRAFLGWRKSWLSREIIAFGLFAK
                     LGAVSLFFPSKLTLSLAALAGVLAVFTSVMVYVDTRRPFWSLKLTGGKFFGTMFLLGT
                     AVPGVIWAWMGQPLAQIAIPTALVLRWLVSLFEMREIKLARRDASSPWHMSARILTEK
                     LPHLLRARKILLAIVGIVLPTVIVTQFQVAAVFTLSVAITLCSQLIERHYFFTACHGP
                     KIPGN"
     gene            4664..6829
                     /locus_tag="PLP58_05415"
     CDS             4664..6829
                     /locus_tag="PLP58_05415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007415860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase"
                     /protein_id="HRJ07986.1"
                     /translation="MNFREWSGPLTADLVRKPAKFGLGQLPARRMPDATTKAVCGFCS
                     TGCGLNIHLKDGAAINLTPDPNYPVNLGTACPKGWEALSVLDAPDRAKMPLLHGKEVD
                     WDTALRYFCDQMKRILDQHGPEAAAFLSTGQIMFEEMAFLGALAKFGMGMIHGDGNTR
                     QCMATAVAAYKESFGFDAPPFTYADFEESDVLVFIGANPCIAHPIMWQRVLRNPRSPE
                     IIVVDPRTTETAMAATQHYAIKPKSDLTLLYGLAHLIIQAGGVDQAFVRASTNGFDEF
                     AAFAADFTPERVAAETGIGIERLRHFAQTIMNGTPRVSFWWTMGVNQGHQATRTAQAI
                     INLALMTGNIGKPGTGANSITGQCNAMGSRLFSNTTNLLGGHDFRNAAHREKVAKATG
                     IPVERIPDRPSLAYDQIIQAADEGTVKAIWFVATNPSHSWIDQNAMNRILGKLDFLVV
                     QDMYCSTETAKRAHLVLPAAGWGEKEGCFINSERRIGYSPKVRRAPGQALADFHIFRL
                     IAHYWDCGEMFREWQTPESVFRVLQRCSEGQPCEVTGIEGYDMIRQHGGVQWPLKKEQ
                     SMQFKTERRLFEDGSFYTTDKRAKFCFDEPVAPPEQCSAEFPLILMTGRGTSAQWHTE
                     TRTAKSAVLARMVPDELMLDLNVQDASSLRVRDGQSVRVTSPRATLTAKARLTACVKP
                     GEVYLPMHDSRVNQLTHASFDPHSRQPSYKHSAVRVEAI"
     gene            complement(6843..7754)
                     /locus_tag="PLP58_05420"
     CDS             complement(6843..7754)
                     /locus_tag="PLP58_05420"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HRJ07987.1"
                     /translation="MPITLPALTRRHWLQGSLAAMLAPRSWGQKAAAEAETWILFADT
                     HIAEDAAREARGVVMAENLTRCVNQVLKNREKPFGVMINGDCAYLDGQQGDYETFAGL
                     VRPLREAGIAVHCTLGNHDDRGHFVEAFTNEQDPKPVPGKHVSVISSARVNWVLLDSL
                     DQVNSTPGILGDAQLGWLDRTLSKLPAKPTLVLAHHNPQGAVAEGQKHTGLTDTDAML
                     KVLDKHSAKVRAFIYGHTHTWSHNVDKATGIHFVNLPPVAYVFNAARPNGWVAARAFE
                     DRLELELRALNPAHDQHKQVVSIPFEG"
     gene            7928..8848
                     /gene="aroE"
                     /locus_tag="PLP58_05425"
     CDS             7928..8848
                     /gene="aroE"
                     /locus_tag="PLP58_05425"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: protein motif:HMM:TIGR00507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004764 - shikimate 3-dehydrogenase
                     (NADP+) activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HRJ07988.1"
                     /translation="MSDFYTFEQLRNWQETAPSLAPPARFAVIGDPVGHSKSPQMHNP
                     ALKACGVDAQYIRVQVPAGRVREAFELFARHGFGGVNITIPHKQEAMAAVDEVDTLAR
                     QLGAVNTLVIRNGSFHGHNTDGPGFIRSVREAFGADVRDLSVLVLGAAGGAGRAVVMQ
                     CLLSGCWKLLIANRSQDRLLELEKEMNALRQGRVPRRRIIRKNWDPASLALAVREADL
                     IVNATSLGMKDGDPALLPGMCLSPWHLVYDMVYRAGGDTPLIADAKAAGARHADGMSL
                     LLHQGAIAFEHWFPGVTAPLEVMRAGLAAA"
     gene            complement(8858..9586)
                     /locus_tag="PLP58_05430"
     CDS             complement(8858..9586)
                     /locus_tag="PLP58_05430"
                     /inference="COORDINATES: protein motif:HMM:NF012863.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDSL-type esterase/lipase family protein"
                     /protein_id="HRJ07989.1"
                     /translation="MRSILILSMAAAFTATHGQEPDVKTGGIVMFGDSTTAPRATISK
                     VYAARVEETLRGGGFSLPVLNAGVGGNTTRDALDRLNQDVLRHRPRVVVIQFGINDAA
                     VDVWKKPPVTTPRVPLAEFLRNIRRMISLAREQDARVILMTTNPLRWTSRMRELYGKP
                     PYDPHADDGFDALHLAVYNDALRALASELKAPLVDVRAAYPAFAAGHGTTVDGLLLDG
                     VHPNDLGHQLVAELLAPPLLEMLR"
     gene            complement(9601..10485)
                     /locus_tag="PLP58_05435"
     CDS             complement(9601..10485)
                     /locus_tag="PLP58_05435"
                     /inference="COORDINATES: protein motif:HMM:NF013357.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003796 - lysozyme activity [Evidence
                     IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA];
                     GO_process: GO:0016998 - cell wall macromolecule catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GH25 family lysozyme"
                     /protein_id="HRJ07990.1"
                     /translation="MNLRLYLPASAFVLSQCAAPAPEGVISPSPAATRGLPQIINVSA
                     YDPKERQRAGRGYSEHDVSALRANGAVALIARAGKGGNLDTKCAQFLAAADRSGMLPG
                     VYYRLQTHVDAVAQADQFINRAQSLARSRGWNAPALLLCGDFDAQSRLSDILRFMDRV
                     EARTGVVPVAYLENSTHLKDLLGGADAATKAKLRRMPYWLALYAHDSGAGPRYPAPGS
                     PGGLLKQYRVWSDWALWQYGGVDWARGRSQPRVYHHGPWRFPLYFGDLDRPVERNVFN
                     GSHAELQAFWRRHGLPLR"
     gene            10608..11660
                     /locus_tag="PLP58_05440"
     CDS             10608..11660
                     /locus_tag="PLP58_05440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007414283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mrp/NBP35 family ATP-binding protein"
                     /protein_id="HRJ07991.1"
                     /translation="MPTESDIRAALSQVRYPGFSRDIVSFGLVKGIQVQDGRIVVGIS
                     LATRDPNVPRLIHEQAMDILQKLAGEGNVRLDFDIKDPPNAAGSSSDSLGKASIPGVK
                     RVIAVASGKGGVGKSTVASNLAVALAKTGARTGLCDCDLYGPSIAHMFGTTERPYQNE
                     EQQIVPVEKHGLRLMSMGLLLEDDSPVIVRGPVATRYTQQFLRQCAWDDLDYLVLDLP
                     PGTGDIQLTIVQTVALDGAVIVTTPQEVALIDARKAVAMFQKVNVPILGIIENMSFFV
                     CPGDGQVYHIFGKGGGEHEAKRLGVPLLGQIPIEMSVRECGDEGHPIALEDAEKSQAS
                     RAFQEIAVNVRAVTPA"
     gene            complement(11667..12971)
                     /locus_tag="PLP58_05445"
     CDS             complement(11667..12971)
                     /locus_tag="PLP58_05445"
                     /inference="COORDINATES: protein motif:HMM:NF024753.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HRJ07992.1"
                     /translation="MTRALFLFATVLAATSSKAADWPEWRGPGGQGIADAKGLPVTFS
                     ETSGITWRAALPGRGHSTPVISGPHVWLTSAVEKAADPAEAERRLKANTGDQPVTVLA
                     SVSLRALCVDLGSGRLLHDIELLNVGEPQWAHQLNSYASPSPILEDGRLHAHFGSFGT
                     ACLDTATGRVLWTNQELRVMHENGPGSSPVLWENLLIIHFDGSDTQFIAALDKDTGRV
                     AWKTPRSGEMDPRPQQRKAYGTPLIVEMNGKPVVVSPAANWIYGYEPATGRELWKVAY
                     DGLGFSMSVRPVADAERVYFSTSYGKSQVIALRYAGLETPEIAWRVTKNAPKMSSPVL
                     AGGLLYFVDDGGILTCVDAATGVDFYRERLGGKYSASPIVADGRLWFASREGVVTVVP
                     LGREFRIESQNTLDGPIMASPAAVDGALYLRTDAALYRVGGK"
     gene            complement(13180..14259)
                     /locus_tag="PLP58_05450"
     CDS             complement(13180..14259)
                     /locus_tag="PLP58_05450"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009958194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialidase family protein"
                     /protein_id="HRJ07993.1"
                     /translation="MSAQFEVLETRVISQQPDLYCGWSTVALRSNGELWLTWSGGREA
                     HVCPFGQVVGMTSKDDGKTWTHPRVLLDSATDDRDSGVLETAKGSLLVTTFTSLAYDP
                     ILKKAEAAGDWPAARLAAWQGARDRLDEAGRKAELGEWLIRSTDGGKSWSGRIPTVVN
                     SPHGPIQLKDGRLLYPGKQLWTGERKIGVCESRDDGLTWQWLAEIPTRPGDDAAGSYH
                     ELHGVEAADGSIIVQIRNHNAENKGETLQTESADGGRTWSVPHSIGVWGLPSHLLRLR
                     DGRLLMSYGHRRPPFGNQARISADHGRTWSEAMIISGDGAGGDLGYPSTVELADGSLL
                     TVWYEKLKESPHAALRQARWRIKPD"
     gene            14527..15273
                     /locus_tag="PLP58_05455"
     CDS             14527..15273
                     /locus_tag="PLP58_05455"
                     /inference="COORDINATES: protein motif:HMM:NF016430.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor"
                     /protein_id="HRJ07994.1"
                     /translation="MAFPTTRWTFFAQATLHGDGAGRVALARMCEDYRRPVEMFLAAR
                     GHLPQEVEDLTQEFFLRWLKSRAWKRADRMKGKFRTFMLAALNHLVAHEAAKKAALKR
                     GGGVMVQRFDEDIFDAEQPAKLEADGCESFDCAWAVALVSHALAALQAEFVARDKERE
                     FEVLRQFLPGSASPPSLEEAAADMGTNVNAFKAALHRLRERFRELLRTEVAKTVSAPH
                     EVEEELQYLRSLLLNLPPQRILEAENGKKT"
     gene            15276..18500
                     /locus_tag="PLP58_05460"
     CDS             15276..18500
                     /locus_tag="PLP58_05460"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HRJ07995.1"
                     /translation="MPDSTPCPARCPACGAVLDPAVVGGACVACLLGEAMETTKATSG
                     LGSIAGHELIEVIARGGMGIVYRVRQRDPRREVALKALPGAELMSGEAKQRFRIEAQA
                     MARLQHPAIVPIYELGEEDGTPFFTMKLAAGGTLSQRIGGYAGKWREIAELMARIAEA
                     VHYAHSRGVLHRDLKPGNILFDEEGHAMVSDFGLAKMIGSDNDLTKTLAMMGTPNYMA
                     PELVRKNGGASTASDVWSLGVILYELLAGRVPFTGDNVPAVLRAVAEDEPAGLAARRT
                     EAGNDERLTATPYSERIPRDLGVIALKALQKEPARRYGTAQEFADDLRRWLAGEPIHA
                     RPVAVAERVWLWAKRKPALAAALALLTLTLLGSTFLLLRSNENLRAVDAQRRAQIHRA
                     LLEKADAERQSMTPGRRGRTLALVREALAHGPSVQARSVAASALAVMDVKVEREWPVG
                     RVGFGQSPMAFTPDLSQHIAILPAAEVRDRAWKQGALGLRRTSDGVLLKQIPLTNRAY
                     YSYASLSQDARWLVTSTRRSKLEIWHLEKETLHTTLTSTTQPAAAFLPSDGTLIAALD
                     GALVRLRLPDLRREVITTGISAVGRLFPSPDAQYLAVYCFDETGTIEGGWIEVRSLAD
                     GRVLARQDQPYGDAGWSNDGQSVVMFHYPTGYLLRHSLDDASSQPLALLRTVGTGRRA
                     AFRSGDRLLGWSDNENFVHLHDLWVGQPTLVIPGTAMNLKFSADGARLAWNPDSESAA
                     IAQVLDPPVLEQRKAVGHERSVDRHLAVSPDGRWIATCDLFGLTLWRAADLQPVAWRF
                     AQRPRDPFESLHFSSDSRHIRFYCGTSKHVNWDVIEAADGTMTLSGPGPEDVPSPHYL
                     QAASPDGRWQITFRADEKNAISHLWEDGKIVSKRQRGPEPGLTSRRNCLVSPDGRWWA
                     AGAWNRDRSRLPGFIYVQSTEVDDSRILLSEAACHTYLAVSPDGRWLLGGEGENYAIW
                     ESGTWKQVFTLPAGLSDAVPGSAAFSPDGRLLALEVDHGKIRLLRVGTWDEVLTITPP
                     QDLPLVLMAFSPDGRHLYTTGGQILHRWDVARLREELERMGLGW"
     gene            18650..27586
                     /locus_tag="PLP58_05465"
     CDS             18650..27586
                     /locus_tag="PLP58_05465"
                     /inference="COORDINATES: protein motif:HMM:NF012200.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choice-of-anchor D domain-containing protein"
                     /protein_id="HRJ07996.1"
                     /translation="MHSFRSAIAPAAMFARLTSAVLVALLPAATFAADHTSTWNGSSG
                     NWTDTTRWTTPTAPGTFPNNGSPTPTYDAILNGGVITVNQDVVIQKLTQSGGTNSGTG
                     FALTTNELLTLSGGVVSGTGTIHVLGGAVIGPVEMSGRTLNFGDGSGPVVNTTWSSGD
                     PLVLGGLVINNRSDSNFIISFDGGSFRNINRSGSVVFSNQGTFTKNGGAGTANIGETN
                     NGSSSFAFNNSGTVVVNTGKLVIGSNGGYPNTHTSTGTFQVADVATLELAGSHTLSTN
                     SLFTGAGTVNFSGGVTATAATTVNTSLTLSGSLGGSGPVTVNGNLLLTGSMNGGNATA
                     LGDVTIAMASQRVISGGAVLNLGDGAGPDEGSTTAWSSGNIDLQSNGTVNNRSDSVIN
                     ATANGTMSNTHNSSTTAFNNQGTFNQNGTGTTTLGAGQGPFVFQNSGLVQVNSGTLVI
                     AGTTGTNNTGSFAVAGGATLNLGGSMNLNAGTTFTGAGTVVFSGGITMNTPITVNPHL
                     TITGTLGGTSPVTAAGNLTFSGGMINDGGNLTALGSVLLNGGDRQIGNAVLNLGDGAG
                     PDAGSTTTWSAGNFTFSGRGGVALNNRSDSVLTTTFDGKFTQIYNGDPASSVINNAGV
                     FTKSGGAGSTVIGDGFYAVTFTNSGTVNANSGTIRFNHGYTQTAGALVLNGGTVNRNG
                     GNFDIRGGKLQGGGTVTGGVTATGGTIHIEPGIGTTPGALTITGDLTLTSGSKLSFDI
                     GGTTPGTEHDKITEGGSGAPNVTGVTLEVNLLNGFIPAAGTELIVLDASHTISGSFAN
                     VANGARLPLPGGLYSCKVSYGASSSFADKTQVILSEFATTPTSLELDDNEIVENATGG
                     STVGLLSATDPDTNETFSFTLVSGTGSTDNGFFQITGNQLQTKALPTPTVVDFETKTS
                     YSVRLRVTNSRGGFLEQNFTLQVLDVPAPSSLDLDTLSVPENQPLNTVIGTLTATDPM
                     PGATFTYELPDSADRISLNILDDKLRSSKVFDKEGSGGFRGPFTARVRVTSSSGEFLE
                     RTFTITVDDQGPSNITLSPAFIPEGRPAGTPVGTLSATVVPEGTGVTYSLVGGIGSTH
                     NAQFMVEGALIKTTAELHVADGTERSIRVRATDANGEIAEQILTITVQVVQVTDILLA
                     PPSVKERLPAGTEVGLLTAVATPEGLPATFELVAGDGDDDNLLFAINGGVLETTVELD
                     EEEQDMRNVRVRTTVSGISFEKALVIQVLDNDPNLVLEHPEGTVLAVFPAGDAVQFPV
                     TEAGMTSEPKVFTIRNTGTGPLKDIEIPGMNHFTVDVTGMAEEVAPGETTTFTVSFSP
                     QTPAATTGPESAVGENMLILSNDPDSGMGGDEFSLSGRAMTAATLAIEQPAGTVINDG
                     SSLYFGLGLIGADGAPRTFTLTNSGEAPVRGLAVSLQGVHEGSFVLDAAGVPDVLPGG
                     GSTTFTVLMHPEEEGALTATLRIRSDSFGDTDINLNGEGLTPEQIAHQAYVKASNTDS
                     GDQFGYSVAVDGDTVVIGARAESSNARGINGDQGNDSSGLSGAAYVLVRTGGAWVQQA
                     YLKASNTDSGDDFGVSVAIEGDTIVVGAAGERSAATGVNGNEADNTAFQAGAAYVFIR
                     QNGVWSQQAYLKASDTSASAHFGFAVAISGETVAVGAHGAASFGSSTGAVYVFTRSGA
                     VWSQEGGPLRPGSSGDGNDFGWSIALDGDLLVAGAPGDDSSIPGINQGSDDDAEDAGA
                     AYVFQRSGGNWGEQAMLKASNAESGDGFGRSVAISGGIVVVGAFNEDSAARGVNGDQA
                     SNGAPDSGAAYVFALDGFNWQQEAYLKASNTGDADRFGYSVAIHRDRLAVGAFREGGS
                     GTGVDPLDDDGVNRAGAVYLFRRAPSFAPDQATNFSWVQNSYLKASNTDAFDSFGLSL
                     ALSEEHLIVGAPQEDSAATGVDGDGSDNSATIAGAAYIFALESRAIQVYAGAELTADR
                     VHSGQAAPVDLGGSPPAVPLTRTFTILNDGLLPLDISAVHVSGDFFVVDQGDPALPGF
                     GAMQFTVQNEPGTSGPLNGTVQIESDDPDLPLFTFTLAGLVGDAPPTEIAIEQPEGAN
                     LTDGASTVNYGALTYSRTAARAFTVRNLGTQPLYIGAITITGADMADFAAGPPGQSEV
                     QPGQATSFMVTFDPTGTVGGSRTAVLHVFSNDTDEGSFDITLTGTVQVPDIEVTGGTI
                     VGGVPTLDFGTVVFGPGKPGRDLAIGVFNVGQGVLEDFNFNIEGVHASDFSIINSAIF
                     TSFGGAAQLAVRFLPQEEGPRSAVLRVESNDPDENPLLINLAGNGNALPVITQQPQSV
                     LVFTGESVTFTAAATGSGLTYQWLKDGIPFGSATPSLTIPNVLATSKGTYSCRVSNSA
                     GVTFSSVAHLAVMASSPAPFPVQANEDTTNILSMASVSPPAQTKILYHWQKDGADLSD
                     GGTAPVISGAFTRSLRLTGVKPASSGTYTCKVTLISSNGGGNLTRVGRTIVLTVGAKP
                     VIAAGGPFEWGAATPVSDVITVTQNPGGIRYSGHPTGVRLSITGAGQLTFQGKPTRIG
                     SGTITVTASNAAGTSLPQTFNYTVAALPANVVGTFDGLVDRSSLFGLHNLGGKISVTV
                     GSNGSWSAALLLGPKVYSLTASPATSNTRFSGGIFSGRAVVNRSSEGLPSLNLDIEID
                     ASTGNLTGTLTDAALTSPQPGEVALEAFKGSVLPQFSYTAALEVSSALAGNPSVPQGN
                     GFFTAKAGSNSTLTMAFKLADSTVKTYAGPVVAGARIPVFFSLYGNVPSSAGSVHGWI
                     TRTADTVPQVNGGRPLLNGALTWLKHPQPSSSTTRSYKEGFPLHTLTVLGGQYAPPPA
                     GSVVLGLQAKTSNARFAFAEGGIEFSALGGSGSPAGTPAGIIRQVCTISTSNLLTLPA
                     INTAQNPANVRVNSFDAATGLFRGTFILRDNDPTDLLPPITQLSRNTAFEGVLVPRLD
                     RGAGFFNLILLPVPGRTLSTMPMLSGQVILEANP"
     gene            complement(27667..28611)
                     /gene="lgt"
                     /locus_tag="PLP58_05470"
     CDS             complement(27667..28611)
                     /gene="lgt"
                     /locus_tag="PLP58_05470"
                     /EC_number="2.5.1.145"
                     /inference="COORDINATES: protein motif:HMM:TIGR00544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HRJ07997.1"
                     /translation="MNLLAYYLHDLSPHLLRITDGIAIHWYGLAYVLGFYLTYLVMVF
                     LARRGLSQIKPAQVADFITLVALFGVVLGGRLGYMLLYDFENFIRAPWSFFLLNQGGM
                     ASHGGIAGVSLFCLWYARKHKISWTGIGDTLVCGAPLGIFCGRIANFINGELFGRVTA
                     FPLAVKFPAELLHEDFAAQGGAPVALPPGVVHSPDIIAYFQGQPGGVAELEAMLHPRH
                     PSQLYEALGEGLLLSAVLLFIRIRFPRLGHGVLTGLFFLLYAAARISLECVRQPDSGA
                     NPILGLTRGQFFSVFMIVVGAAFLVHGLRRGGAGKSPV"
     gene            complement(28662..29258)
                     /locus_tag="PLP58_05475"
     CDS             complement(28662..29258)
                     /locus_tag="PLP58_05475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ07998.1"
                     /translation="MTPANEQQRVSILFTGILVCVPVLALHRFGLFPEMALWLEERLP
                     RLLVLPEHGLRMSLPLQYACFTALAFLSARTGLARGGLWLKMIYLLAVTHLTVCLAFT
                     LAWAGILFEPFSGVLAAWAACLVAVIVADLEQRRPETPEPEIAKEAPAARKTTLAQIV
                     EAGVPGAPETDAKPDAPTVTGSPDNPAKNNGPVIVAKA"
     gene            complement(29255..30895)
                     /locus_tag="PLP58_05480"
     CDS             complement(29255..30895)
                     /locus_tag="PLP58_05480"
                     /inference="COORDINATES: protein motif:HMM:NF019344.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HRJ07999.1"
                     /translation="MKEDIENKPDEPPAQPPAKPEEITRKLEDFIKNTLGGQVLFSRV
                     EPSMPGLRPHRGDPEPVEEAEAEPEFDFRLKPADIKAYLDRFVIRQDEAKKVLATAVC
                     DHYNHARMISEERKQRGGDGPALEFSKQNVLIAGPTGVGKTYLVKHIADLIGVPFVKA
                     DATKFSETGYVGADVDDLVRDLVAKAGGDVRLAGHGIIYLDEIDKLATGGEGRIGRDV
                     SGRGVQTALLKLMEETEVPLYAPNDIRGQMQMMFDTRKGRTGREVVNTRNILFIVSGA
                     FSGLEKIIEKRQSRASIGFGASVGGKPAAEDLLREAGTRDFIDYGFEAEFIGRLPVRV
                     FCDALGADDLFHIMKNSEGSLIRQFEREFHAYGVRALFEDDALREVATRAAGEKTGAR
                     GLVTAWEKVLREFKFEMPSLGLPELIVDKALVLNPSAALEHSREAALSLRTDARAGEV
                     RAFAARFTREHGLELVFDAGAVQGLVRRAEREGSGVEELCSRLFKDFPFGLQLVARNT
                     ARKVFHLGPEAVERPDKFLSDLVVNCYRGAEPAPSPES"
     gene            31006..>31439
                     /locus_tag="PLP58_05485"
     CDS             31006..>31439
                     /locus_tag="PLP58_05485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ08000.1"
                     /translation="MSAPPQSILSVFNRYRHPGGEEMSVERIAGLLSTRHHVANCRFE
                     SSEWTGPGAPSTLGQAWRLFYNPEGVRRFESALDKSGAALALLHNIFPVGSPAIYRSA
                     RRRGLPLMQFMHNYRPFSVGGTLYSRGRILKDPLFGHYWREVA"
BASE COUNT         5993 a         9959 c         9862 g         5625 t
ORIGIN      
        1 ccgccgatct cgtcaaagac aagaacgtcc tcctcatcac ctccacctgg ggtgagggcg
       61 acatgcctga caacgcggtg tccttctggg acgccatcaa tcaaaacggc agcagcccca
      121 agctcgacgg cgtcaaatac agcgtccttg ctctcggcga caaaaactac ggcgacacct
      181 tctgcctcgc cgggaagaaa ctcgatcaac gcctcgccga gctcggcgcc actcgcgtcg
      241 tcgatcgcgt ggactgcgat gtggacttcg acgacctcgc caaagaatgg tcgaagaatg
      301 ctttcaccat cttcggagcc tccaagccgt ccgacgtgtc cgacatgtct gacccgtccg
      361 acaaagcgtg gtccaaaaag aatcccttcc ccgccaaact catcgccaac gacacactaa
      421 acgcacccgg cagcgccaaa gatacgcatc acatcgcgtt ctcgctcacc ggcagcggcc
      481 tcacctacga ggtcggagac gctctcggcg tgttcgtcca aaactgcccc gaagtcgtgg
      541 atgccgtcat cgccgcccac ggcttcgacc caagcgccaa agccggcacc aaaacgctga
      601 gtgaggcgct cctcaccgat tacgaaatcc gcagccttct cggcaaaacg cccgagcccg
      661 gcctcacgct cgattccttc ctcgaaaatc tccgcaaact ccagccgcgc ctctattcca
      721 tcgccagcag catcaaggct catcccgatg aagtgcatct ctgcgtcggc gcggtgcgct
      781 acaccgccga tggcatcgcg cacaaaggcg tcgccagcac attcctctcg gatcgcttcc
      841 cactcggcga caccaccggc atctacttcc acgccgcgaa ccacttccgc ctccccaccg
      901 acctcaccaa gcccgtcatc atggtcggcc ccggcaccgg catcgcgccg ttccgcgcct
      961 tcctcgaaga acgagaggcc accaaggctc ccggcaaaaa ctggctcttc ttcggcgatc
     1021 aaaaacgcgc caccgacttc ctttaccacg accaaatcat cgcctgggtg caaagcggcc
     1081 atctcacccg actcgacacc gcattcagtc gcgatcagga ggaaaaaatc tacgtccaga
     1141 cccgcatgct ccaggccgcc agcgaactct ggcaatggct cgaagaaggc gcccacttct
     1201 acgtctgcgg cgatgccaaa cgcatggcca aagacgttga tgacgccctc cacaagatca
     1261 tcgagaccca aggcaacaaa accgccgacg aggccgccgc ctacgtcgcc cacatgaaga
     1321 aaaccaaacg ctatgcccgc gatgtctatt gatgcaccac gcgttgccag cgctcaagtt
     1381 ggagaggatt tctccaatga gcagaagcgc tacctcgaag gtttcttcgc cggcatcgca
     1441 gcgggtggag tcagttttgg cgatctctcg ccggctcccg ccgagcccgc aggcccctca
     1501 ctcgacgatt tgaccaagga ggagcgcatc aagcgcgaac tccatccctt cgatgccatt
     1561 gagcagctcg tgctcgatgc gcgctggaac gcgaagccag aacctgaatc cgtcttccgc
     1621 tacaaatgga acggcctgtt ctggctcaat cccgtcaaag acggctacat gtgcagactg
     1681 cgcattcctg gcggcgtggt gaaaagctac cagcttcgtg aactggccgc catttcacgc
     1741 gagctgacct cgggttacat ccagatcacc acacggaaca actttcaaat ccgtctcatt
     1801 gagcccaaga gctgccctga agtgctccgc cgcatccagg gctgcggctt gcactcgaag
     1861 ggcgctggcg cggacaacat ccgcaacatc acgatgaacc cctgtgcggg ttacgatccg
     1921 catgagctca ttgacgtgcg accgctggtg aacgagctcg ccacgctcat catcggctcg
     1981 aaggagttct acgacctgcc gcgcaagttc aacatcgcct acgacggcgg cgggctcatc
     2041 tcatcggtcg aggacacgaa cgacatcggt gccagcgccg tgaagatcat tgaaaacagt
     2101 gaaagcatcg agcccggcgt ctatttccgc atcgcgctcg gcggcgtcac cggtcaccag
     2161 accttcgcca gcgattgggg cgtcatcgtg aaacccgaac agctcaacga tgtcatggtg
     2221 gccctgctgc gcgtgttcat cgagttcggt gaccgcacga accgcaagaa ggcccgcttc
     2281 aaatatgtcg tggaggacaa aggtctcgat ggcgtgcttg tggaggccga gaagcatctc
     2341 aaattcaaac tcacccgcct cgcgcccgat tcaccgatcc aggcgaagcg cgagttctcg
     2401 cagcaaggcc actcgcacgt tggcatccat ccgcagaagc aggacggact cgtttacgtc
     2461 ggggccagcg tgccagtcgg ccagctcacg gcaaagcaaa tggagcgcat cgccgatctc
     2521 gccgacagct acggcagcgg cgaggtgcgc ttgaccgttt ggcagaattt catcatcccg
     2581 aatgtgaaga ctgcctatgc tgccacactc gcaaaatcgc tcaaaaagct cggctttccc
     2641 tgcgagcagt cgaatctgaa aagcggcttc gtcgcctgca ccggcaacag atactgcaaa
     2701 tacgccgcca gcgacacaaa gggccacgcc atcgccatga tggactacct cgacaagcgc
     2761 gtgaagctcg acaagcccgt gaacatccac ttcaccggct gcgcgcactc ctgcgcccag
     2821 cacttcatgg gcgacatcgg cctgctcggc accaaggtga aatctgaaag cggcgaaggc
     2881 tatcacatca ccgtcggcgg cggtttcggc gagaaccgca agatcggccg ccagattttc
     2941 agcggcgttc ccttcgaaag cctcggcgcc actttggaag ccatgctgaa aggctatctc
     3001 aaaagccggc agggcgagga gagctttcac agcttctgca accaaagaac catcggccag
     3061 cttcaggagg cattctcggt tgcatgacca tggcgttgtc agacttgcac gacctgtctg
     3121 accggtcaga cctgctgcac aaggtgctcc gggagcagca aacgctccgc acacccgtcg
     3181 ctctcttcgc ggaggaatac gattcaaagt ccgtccgcca gcgcttcacc cacctgatcc
     3241 cgctttcgaa gccaaatccg ggcgagcagt atgctttcca ggtaaatctg gacgcctgca
     3301 ccggctgcaa agcctgcgtc gcggcctgcc attcgctcaa tggactcgat gaggatgaat
     3361 catggcgcga tatcggcctg cttgttggca cgcggaagca gccctatctg cagaccgtca
     3421 cgacggcctg tcatcattgt gaggatcccg cctgctcgaa tgggtgcccg gtgctcgcct
     3481 atgacaaaga tcccgtcacc ggcatcgtgc ggcatctgga tgaccagtgc atcggctgct
     3541 cctactgcat cctgaagtgc ccgtatgatg tgccgaagtt caacctgaag cgcggcatcg
     3601 tgcgcaagtg cgacatgtgc cagggccgcc tcgcggaagg cgaagcacct gcctgcgtgc
     3661 aagcctgtcc gaacgaggcc atcaaaatcc aagtcgtgaa actcagcgac gtgcctgatc
     3721 gcggccagat catcgccgga gcgtttgatt cgagctacac gcgacccacc acgacttaca
     3781 cgtcctccaa atcgcttccc gaagctaaac cggccgatga aggccagctc cgcctcgatc
     3841 atgcacacac gccgctgacg tggatgcttg tgctcaccca aatgagcacg ggaaccttcc
     3901 tgatcaatgc cctagtggtg tggatgggct actttacggc agaagacacc cgagtgacct
     3961 tgttgacggc attgacgatg ggtgtcgttg gcatcgcatt gagcgttttg catctcgggc
     4021 agccgttgaa ggcttggcgg gcgtttttag gctggcgcaa atcctggctc tcccgcgaaa
     4081 tcatcgcctt cgggctcttc gcgaagctgg gggctgtttc gctgtttttt ccatccaagc
     4141 tcacgttgag cctcgcggcg cttgcgggtg ttttggccgt gttcacctcc gtcatggtct
     4201 atgtggacac gcggcggcca ttttggtcgc tgaagctcac cggcggcaaa ttcttcggca
     4261 cgatgtttct gctcggcact gccgtccccg gcgtgatttg ggcctggatg ggccagccgc
     4321 tggcgcaaat cgccattccg acggctctcg ttctgcgctg gctcgtttca ctttttgaga
     4381 tgcgcgagat caagctggct cgtcgtgatg catcgagccc ctggcacatg tccgcccgta
     4441 ttttgaccga aaaactgcca catctgcttc gcgcccgcaa aatcttgctc gccatcgttg
     4501 gcatcgtttt gcctacggtc attgtcacgc aattccaagt cgccgctgtg ttcactttgt
     4561 ccgtcgccat cacgctgtgc agccagttga tcgagcgaca ctacttcttc actgcctgcc
     4621 acgggcctaa aataccgggg aactaatgct cacatttccc gccatgaatt ttcgcgaatg
     4681 gagcggccct cttaccgctg acctggtccg caagcctgcg aagtttggcc ttgggcagct
     4741 gcccgcgcgg cggatgccgg atgcgacgac aaaagcggtg tgcggtttct gctccaccgg
     4801 ctgcggattg aacatccacc tcaaagacgg cgcggcgatc aatctcactc ccgatcccaa
     4861 ctatcccgtg aacctcggca cggcttgtcc gaagggctgg gaagcgctga gcgtgcttga
     4921 tgcgccggat cgggccaaaa tgccactgct gcatggcaag gaagtcgatt gggacaccgc
     4981 gctgcgttac ttctgcgacc agatgaagcg catcctcgac cagcatggcc ccgaggccgc
     5041 cgcgtttctc agcacggggc agatcatgtt cgaggagatg gccttcctcg gcgctttggc
     5101 aaagttcggc atgggcatga tccacggcga tggcaacacg cgccagtgca tggccactgc
     5161 ggtcgccgct tacaaggaaa gcttcggctt cgacgcgccg cccttcacct acgccgactt
     5221 tgaagagagc gatgtgctcg tcttcatcgg cgcaaatccc tgcatcgcgc atccgatcat
     5281 gtggcagcgt gttttgcgca acccgcgcag cccggagatc atcgttgtgg atccgcgcac
     5341 cactgaaacc gccatggcgg ccacgcagca ttatgccatc aagcccaaga gcgatctgac
     5401 gctgctctat ggccttgccc atctcatcat ccaggcaggc ggcgttgatc aagctttcgt
     5461 cagagccagc acgaacgggt ttgacgagtt tgccgccttt gccgctgatt tcacgcctga
     5521 acgcgtcgcc gctgaaacgg gcatcggcat tgaaaggctg cgccattttg cgcagacgat
     5581 catgaacggc acaccgcgtg tgtccttctg gtggacgatg ggcgtcaacc agggccacca
     5641 ggccacgcgc accgcgcagg cgatcatcaa tctggcgctg atgaccggga acatcggcaa
     5701 accgggcacg ggcgcgaatt ccatcaccgg gcagtgcaat gcgatggggt cgcgtctgtt
     5761 ctccaacacg acgaatttgc tcggcgggca tgattttcga aatgccgcgc atcgtgagaa
     5821 ggtcgcgaag gccacgggca tcccggtgga gcgcatcccc gaccgtccca gccttgcgta
     5881 cgatcaaatc atccaggctg ccgatgaagg cacggtgaag gccatctggt ttgtcgccac
     5941 gaaccctagc cattcatgga ttgatcagaa cgcgatgaac cgcatcctcg gcaagctcga
     6001 tttcctcgtc gtgcaggaca tgtattgctc taccgagacc gcaaagcgtg cgcatctcgt
     6061 gctgcctgcg gccggttggg gcgaaaaaga aggctgcttc atcaattcag agcggcgaat
     6121 cggctactca cccaaagtgc gtcgcgctcc cggtcaggcg ctagctgatt ttcacatttt
     6181 ccgcctcatc gcccactact gggactgcgg ggagatgttt cgtgaatggc agacgccgga
     6241 atcggtgttt cgcgtgctgc aacgctgctc cgagggacag ccgtgtgagg tcacgggcat
     6301 cgaaggctac gacatgatcc gccagcatgg cggcgtgcag tggccgttga agaaggagca
     6361 aagcatgcag ttcaaaacgg aacgccgctt gtttgaggat gggagtttct acaccacgga
     6421 caagcgcgcg aagttctgct tcgacgagcc ggtggcaccg ccggaacagt gcagcgccga
     6481 gtttccgctc attttgatga caggccgcgg cacgagcgcc cagtggcaca cggagacccg
     6541 caccgcgaag tcggccgtgc tcgccaggat ggttccagac gagttgatgc tcgatttgaa
     6601 cgtccaagac gcctcctcac tcagggtccg cgacggacaa agtgtgcgag tgacctcccc
     6661 acgggccact ttgaccgcca aagcccgcct gacagcctgc gtcaaacccg gcgaggtcta
     6721 tctgccgatg cacgacagcc gcgtgaacca actcacgcac gccagctttg atccccacag
     6781 ccgccagccg agttacaagc actcggcggt gcgggtggag gcgatttgag cacacgcaaa
     6841 gattaccctt caaaggggat gctgacgact tgcttgtgct ggtcgtgagc gggattgagc
     6901 gcgcgcagtt ccagttccag gcgatcctcg aaggcacgcg cggccaccca gccgttgggg
     6961 cgtgcggcat taaagacata ggccacaggg ggcaggttca cgaagtggat gccggtggct
     7021 ttgtccacgt tgtgcgacca ggtgtgagtg tggccgtaga tgaaggcgcg gactttggcg
     7081 gagtgtttgt cgagcacttt gagcatggcg tccgtgtcgg tgaggccggt gtgtttttgt
     7141 ccttcagcca cggcgccttg ggggttgtgg tgcgcgagga ctaaggttgg cttggcgggc
     7201 agtttggaga gggtgcggtc cagccagccg agttgagcgt cacccaggat gcccggcgtg
     7261 ctgttgacct gatccagaga gtccagcagc acccagttca cccgcgcgct gctgatgacg
     7321 ctgacgtgct tgccaggcac gggcttgggg tcctgctcgt tggtgaaggc ttcgacaaaa
     7381 tgcccgcggt cgtcatgatt ccccagagtg caatgcacag cgatgcccgc ctcgcgcagc
     7441 ggacgcacga gtccggcgaa ggtctcgtag tcgccctgct ggccgtccag gtaggcgcag
     7501 tcaccattga tcatgacccc gaagggcttc tcgcggttct tgagcacttg attgacgcag
     7561 cgtgtgagat tttccgccat gaccacaccg cgcgcctccc tggcggcatc ctccgcgatg
     7621 tgggtatcgg cgaaaagaat ccatgtttca gcttcagcgg cggctttctg cccccatgag
     7681 cgcggtgcga gcatggcggc gaggctgcct tggagccagt gacggcgggt aagggcggga
     7741 agggtgattg gcatggctgg gaggataaac ggcgcatcac actcccggca atccagaact
     7801 tcacttcaag gtgggcgtgg gacaacacag gcgcgccatt tggcaaacca gtcaatctgc
     7861 gtgcaaagcc tcccgcgcac aggcgccagc cttgaccgag cgcgggcctg gcgccaagac
     7921 ttgggccgtg tctgactttt acaccttcga acaattgcgg aactggcagg agacggctcc
     7981 ttccctggct cctccggcgc gctttgccgt gatcggcgat ccagtcggac actccaagtc
     8041 cccgcagatg cacaatccgg cgctcaaagc ctgcggcgtg gacgcgcaat acatccgcgt
     8101 gcaagtgccg gcgggcaggg tgcgcgaggc tttcgaattg ttcgcgcgcc acggcttcgg
     8161 cggggtgaac atcaccatcc cgcacaagca ggaggcgatg gccgctgtgg acgaggtgga
     8221 cactctggca aggcagctcg gagccgtgaa cacgctggtg atccgcaatg gcagtttcca
     8281 cggccacaac acggacggcc ccggctttat acgcagtgtg cgggaggcct tcggtgcgga
     8341 tgtcagagac ctcagcgttc tcgtgctcgg cgcggcgggc ggagcggggc gcgccgtggt
     8401 gatgcagtgc ctgctcagcg gctgctggaa actgctcatc gcaaaccgct cgcaggaccg
     8461 ccttttggag ctggaaaagg aaatgaacgc cctgaggcag ggccgcgtgc cgcgtcgcag
     8521 gatcatccgt aaaaattggg atccagccag tttggcgctg gccgtccggg aggccgacct
     8581 catcgtcaac gccacatccc tcggcatgaa ggacggcgac cccgcgctgc tgccgggcat
     8641 gtgcctctcc ccgtggcatc tcgtctacga catggtctat cgcgccggag gggacacgcc
     8701 tttgatcgcc gatgccaaag ccgcgggcgc gcggcacgca gacggcatga gcctgctgct
     8761 gcaccagggc gcgatcgcct tcgagcactg gttccctggt gtgacagcgc ctctggaggt
     8821 gatgcgcgcc gggctggcgg ctgcctgagc gcgaacttca gcgcagcatt tcaagaagcg
     8881 gcggcgcgag cagctccgcc acgagctgat gaccgagatc gttgggatgc acgccatcga
     8941 gaagcagccc gtccacggtg gtcccatgcc ccgcggcaaa ggcgggataa gcagcgcgca
     9001 catccaccag cggcgctttg agttcggacg ccagggcgcg cagagcgtca ttgtaaacgg
     9061 caagatggag cgcgtcgaaa ccgtcatccg cgtgagggtc ataaggcggc tttccataaa
     9121 gctccctcat gcgcgaagtc cagcgcagcg gattggtggt catgaggatg accctggcgt
     9181 cctgttcgcg cgcgagggag atcatgcgac ggatgttgcg gaggaattca gccagcggca
     9241 ctcgcggcgt ggtgaccggc ggctttttcc acacgtcaac ggcggcgtca ttgatgccga
     9301 actggatgac gaccacgcgc ggcctgtgcc gcagcacatc ctgattgaga cggtcgagcg
     9361 cgtcgcgcgt ggtgttgccg ccgacgccag cgttgagcac gggcaggctg aatccgccgc
     9421 cgcgcaaagt ctcctccacc cgcgcggcat agaccttgga aatggtggcg cgcggcgctg
     9481 tggtggagtc gccaaacatg acgatgccgc ccgttttgac atccggctcc tggccatgcg
     9541 tggcggtgaa ggcggccgcc attgagagga tgaggatgga gcgcatggat ggagttttga
     9601 tcagcgcagc ggcagcccgt gcctgcgcca gaaggcttga agctccgcat gggagccgtt
     9661 gaacacattg cgctccaccg ggcggtcgag atcgccgaaa taaagcggga aacgccacgg
     9721 cccgtgatga tagaccctag gctgcgagcg cccgcgcgcc cagtcgacgc ctccatattg
     9781 ccagagcgcc caatcactcc acacgcgata ctgtttcaac aacccgccgg ggctgcccgg
     9841 cgccgggtag cgcggcccgg ctccgctgtc gtgcgcgtag agggccagcc aatagggcat
     9901 gcggcggagt ttggccttgg tggcggcgtc ggcgccgcca agaaggtctt tcaaatgcgt
     9961 gctgttctcc agataagcca cgggcaccac gcccgttcgc gcctcgacgc ggtccatgaa
    10021 acgcaggatg tcgctgagcc gtgactgggc gtcgaaatcg ccgcagagca ggagcgcggg
    10081 cgcgttccac ccgcgcgagc gcgccagcga ctgcgcgcgg ttgatgaact ggtccgcctg
    10141 cgccaccgcg tccacatggg tctgcaggcg gtaatacaca cccggcagca ttccagaccg
    10201 gtctgcggcg gcgagaaact gcgcgcattt cgtgtccagg ttcccgccct tccctgcgcg
    10261 cgcgatcaat gccaccgcgc cgttggccct cagtgcggag acatcatgct cgctgtagcc
    10321 ccgcccggcg cgctgtcttt ccttcgggtc ataagcagag acattgatga tctgcggcag
    10381 accgcgcgtg gctgctggtg acggggaaat caccccttcg ggcgctggcg cggcgcattg
    10441 gctcaggacg aaggcggagg ctggaaggta aagtcggagg ttcatgccgg cgaagaacga
    10501 acgcgccagc cgccccctca tttcgccgta atgcgttggg cactcattcg aggtccgcgc
    10561 ttgagttttg atctccgccg cctagcggag tcgtaaacca ccgaaccatg cccaccgaat
    10621 ccgacatccg cgccgcgctc tcccaagttc gctaccctgg cttcagccgt gacatcgtct
    10681 cctttggcct tgtgaaaggc atccaagtcc aggacggcag gatcgtcgtc ggcatctcgc
    10741 tggcgacacg cgacccgaac gtgccccggc tcatccatga gcaggccatg gacattctgc
    10801 aaaagctggc cggagagggc aatgtgcgtc tggattttga catcaaagat cccccgaacg
    10861 ctgccggctc atcctccgac agccttggca aagccagcat ccccggagtg aagcgtgtga
    10921 tcgccgtggc ctccggcaag ggaggcgtgg gcaagagcac cgtggcgtcc aatctggccg
    10981 tggccctggc caaaaccgga gcgcgcaccg gcctttgcga ctgcgacctt tacgggccga
    11041 gcatcgcgca catgttcggc accacggagc gtccctatca gaacgaggag cagcagatcg
    11101 tgccggtgga aaaacacggc ctccggctca tgagcatggg cctgctgctg gaagacgact
    11161 cgcccgtgat cgtgcgcggc cccgtcgcca cgcgctacac gcagcagttc ctccgccaat
    11221 gcgcctggga tgacctggac tacctggtgc tcgatcttcc accaggcacg ggggacatcc
    11281 agctcaccat tgtgcagaca gtggcgctcg acggcgctgt catcgtgacc acaccgcagg
    11341 aggtggcatt gatagacgcg cgcaaggcgg tggccatgtt ccagaaggta aacgtgccca
    11401 tcctgggcat cattgagaac atgagtttct tcgtctgccc gggtgacggg caggtttacc
    11461 acatcttcgg caaaggcggc ggcgagcatg aggcgaaacg cctgggtgtg ccgctgctgg
    11521 gccagatccc catcgagatg agcgtgcgcg aatgcggcga cgaggggcat cccatcgcgc
    11581 tggaggacgc ggaaaagtcg caggcatccc gcgcctttca ggagatcgcc gtgaatgtgc
    11641 gcgcggtcac acccgcgtga ggctgctcat tttccaccga cgcggtaaag cgccgcatcg
    11701 gtgcgcagat acagcgcgcc atccacggcc gccggactcg ccatgatcgg cccgtcgagc
    11761 gtgttttgcg actcgatgcg gaactcccgt cccagcggca ccacggtcac cacgccctcg
    11821 cggctggcga accacagcct gccgtccgcg acaatgggtg acgcgctgta ttttcccccg
    11881 agccgctcgc gataaaaatc cacgcccgtg gccgcgtcca cacaggtgag gatgccgccg
    11941 tcatccacga aatacaaaag ccctcccgcc agcacgggcg aggacatttt cggtgcgttc
    12001 ttcgtcaccc tccatgcgat ctccggcgtc tccagacccg cgtaacgcag tgcgatgacc
    12061 tgcgacttgc cgtagctcgt gctgaaataa acgcgctcag catccgccac cggacgcacg
    12121 gacatggaaa agccgagacc gtcgtaggcc accttccaca gctcgcggcc cgtcgccggc
    12181 tcatagccgt aaatccagtt cgccgccgga ctgaccacca cgggtttgcc gttcatttca
    12241 acaatgagcg gcgtgccgta ggccttgcgc tgctgagggc gcggatccat ctcgccgctg
    12301 cgcggggtct tccatgccac cctgccagtg tccttgtcga gcgcggcgat gaactgcgtg
    12361 tcactgccgt cgaagtgaat gatgagcaga ttctcccaca acacggggga cgagccggga
    12421 ccgttttcgt gcatcactcg cagttcctga ttcgtccaga gcaccctgcc cgtggctgtg
    12481 tcgagacagg cggtgccgaa gctgccaaag tgggcgtgaa gcctgccgtc ttcgaggatg
    12541 gggctgggcg aggcgtagct gttgagctga tgcgcccact gaggctcgcc cacattcaac
    12601 agttcgatgt catgcagcag cctgccgctg ccgagatcca cacacaaggc gcgcagactc
    12661 acggaggcga gcacggtcac gggctggtcg ccggtgttcg ccttcaggcg gcgctccgcc
    12721 tcggcgggat cggcggcttt ttcgacagcg gaggtgagcc agacatgcgg gccggagatg
    12781 accggagtgg agtgcccgcg accaggcaag gcagcgcgcc aggtgatgcc gctcgtctcg
    12841 ctgaaagtca cgggcagtcc cttggcatcg gcgatcccct gcccgcccgg cccgcgccac
    12901 tcgggccagt cggcggcttt cgaagaggtt gcggctaaga cggtggcgaa tagaaaaagg
    12961 gcgcgtgtca tcgggcggaa tcaaacgcgc ccgatccgcg cgaactctcc ggcattatgc
    13021 cctgccccgg agaataccgc tggatggcac agagggctcc cgaaaaccca aaatatcggg
    13081 cgcagacatc ctgcctgcgc gaacaatctg gactcctttt ccccatgcat aataaccggg
    13141 ttttcaggag tccgtttcat gcaggtctca aaaccgcgct cagtccggct tgatgcgcca
    13201 gcgcgcctgg cgcagcgcgg cgtgcggact ttccttgagc ttctcatacc acacggtcag
    13261 cagcgagccg tccgccagtt ccaccgtgct cggatagccc aggtcgccgc ctgcgccgtc
    13321 gcctgaaatg atcatcgcct cgctccaagt tcttccgtgg tcggcgctga tgcgggcttg
    13381 attgccaaaa ggcgggcgcc ggtgcccgta ggacatgagc aggcgcccgt cgcgcaggcg
    13441 cagcagatgc gagggcaggc cccacacgcc gatgctgtgc ggcacactcc aggtcctgcc
    13501 tccgtcggcc gactccgtct gcaaagtctc gcctttgttt tcggcgttat ggttgcggat
    13561 ttgcacgatg atcgagccat cagcggcctc cacgccgtgc agttcgtggt aactgcccgc
    13621 cgcgtcatcg ccgggccggg tggggatttc cgccagccat tgccacgtca acccgtcatc
    13681 cctggattcg cagacaccga tcttgcgctc gccggtccaa agctgcttgc cggggtaaag
    13741 caaccgcccg tctttgagct ggatggggcc gtgcgggctg ttgaccacag tcggaatgcg
    13801 gccggaccat gactttccac cgtctgtcga acgaatcagc cactcgccca gttcggcttt
    13861 gcgtccggct tcatcgagcc ggtcgcgcgc gccctgccag gcggcgaggc gcgcggcggg
    13921 ccagtcaccg gcggcctcgg cttttttcaa aatgggatcg taggccagcg aggtgaaggt
    13981 ggtcaccagc aggctgcctt tggccgtctc cagcacgccg ctgtcgcggt catcggtggc
    14041 gctgtcgagc agcacgcgcg ggtgggtcca ggtcttgcca tcgtccttgg aggtcatgcc
    14101 caccacctgg ccgaaggggc agacatgcgc ctcgcgcccg ccggaccagg tgagccatag
    14161 ctcgccattt gaccggagcg ccactgtgga ccagccgcag taaaggtcgg gctgctggga
    14221 gatgacccgg gtttccagga cttcgaactg cgctgacaag gctgaaacag atggaagcag
    14281 ggaaagacaa aaaaacacgg ctttaatggc tggatggctc atgcatcaaa ctaatgagag
    14341 cgggcggcgt gtctttcatc acggcctcag ccagccacac ttcaggcgct catggccgcg
    14401 gcttcagccc ggcggcgcgc gcggcgtcca gccagttgcc cctggcgcaa tgccgcctgg
    14461 aaggattcct ggcagcacac tctgcccccg cttgcacgga cgcatgcgct acataactct
    14521 atccacatgg ccttcccgac cacccgatgg actttctttg cccaagcgac gctgcatggc
    14581 gatggggcgg ggcgtgtggc actggcgcgg atgtgcgagg actaccggcg gccggtggag
    14641 atgtttctgg cggcgcgcgg tcatctgccg caggaggtag aggatctgac gcaggagttt
    14701 tttctcagat ggctcaaatc acgcgcgtgg aagcgcgcgg accggatgaa gggaaagttc
    14761 cgcaccttca tgctggcggc gctgaatcat ctggtggcgc atgaggcggc gaaaaaagcc
    14821 gcgttgaagc gcggcggcgg cgtgatggtg cagaggttcg atgaggacat ctttgatgca
    14881 gaacagcccg cgaaattgga ggcggatgga tgcgagagct ttgactgcgc ctgggcggtg
    14941 gcgctggtga gtcatgcgct ggcggcgctg caggcggagt ttgtggcgcg ggacaaagag
    15001 cgcgagttcg aggtgctgcg gcagtttttg cccggcagcg cgtccccgcc ctcgctggag
    15061 gaggcggcgg cggacatggg cacgaatgtg aatgccttca aagcggcgct gcaccggctg
    15121 cgggagcgtt ttcgcgagtt gttgcgaacg gaggtcgcca aaacggtgtc cgcgccgcat
    15181 gaggtggagg aggagttgca atacctgcgc tccctgctgc tgaatctgcc gccgcagcgt
    15241 atcctggagg cggaaaacgg gaagaagaca tgacgatgcc cgactccacg ccttgcccag
    15301 cacgctgccc ggcctgtgga gccgttttgg accctgccgt cgtcggaggg gcgtgcgtgg
    15361 cctgcctgct gggcgaggcg atggagacga cgaaagccac cagcgggctg ggcagcatcg
    15421 ccgggcatga attgatcgag gtcatcgccc ggggcggcat gggcatcgtg tatcgcgtgc
    15481 ggcagcgtga tccaagacgg gaggtggcgc tgaaggctct tcctggcgcg gagctgatga
    15541 gcggggaggc aaagcagcgc ttccgcatcg aggcgcaggc catggcgcgg ttgcagcatc
    15601 cggccatcgt gcccatctac gagctgggcg aggaggacgg cacgccgttt ttcacgatga
    15661 agctggcggc gggcggcacc ctctcgcaac gcatcggcgg ctatgcgggc aagtggcgcg
    15721 agatcgcgga gctgatggcg cgcatcgcgg aggcggtgca ctacgcgcac agccgcggcg
    15781 tgctgcatcg cgacctgaag ccggggaaca ttctatttga tgaggaaggc cacgcgatgg
    15841 tcagtgactt cggcctggcc aagatgatcg gctcggacaa cgatctgacg aagaccctcg
    15901 cgatgatggg cacgcccaac tacatggctc ccgagctggt gcgtaaaaac ggcggcgcaa
    15961 gcaccgccag cgatgtgtgg agcctgggcg tgatcttgta tgagttgctc gcggggcggg
    16021 tgcccttcac aggggacaat gtgcccgccg tgctgcgagc cgtggcggag gatgagccgg
    16081 cggggcttgc agcccgccgc acggaggcgg gcaacgatga gcgtctcact gcgacgccct
    16141 acagcgagcg catcccgcgc gacctcggcg tcatcgcctt gaaggcgctg caaaaggagc
    16201 ccgcgcgccg ctacggcacg gcgcaggagt tcgcggatga tctgcgccgc tggctggcgg
    16261 gggagccgat ccacgcgcgg cctgtcgcag tggcggagcg cgtgtggctg tgggcgaaac
    16321 ggaaacccgc cctcgccgcc gcgcttgcgc tactcacgct cacacttctc ggcagcacct
    16381 tcctgctgct gcgtagcaat gaaaacctcc gcgccgtgga tgcgcagcgg cgcgcacaga
    16441 tccaccgcgc gctgctggag aaggcggacg ccgagcgcca gtccatgaca ccgggccgtc
    16501 gcgggcgcac gctggccctg gtacgcgagg cactggcgca cggcccctcg gtgcaggccc
    16561 gctccgtggc tgcatccgca ctggcggtga tggatgtgaa ggtggagcgt gaatggcccg
    16621 tcgggcgcgt aggcttcggc cagagcccga tggcatttac ccctgatctc tcccagcaca
    16681 tcgccatcct gcctgcggct gaagtccgtg acagagcatg gaaacaaggc gcgctgggac
    16741 tccgccgcac cagcgatggc gtgctgctga agcaaatccc cctcacgaac cgcgcctatt
    16801 attcttacgc ctcactgagc caggatgcac gctggctggt caccagcacc agacgctcaa
    16861 aactggagat ctggcatctg gagaaagaga cgctgcacac caccttgacg agcaccacgc
    16921 agcctgcggc ggcattcctg ccatcggacg gcaccctgat cgcagcgctc gatggtgcgc
    16981 tggtgcggct gcgtctgcct gatttgcggc gggaggtcat caccaccgga atctccgctg
    17041 tgggcaggct gtttcccagt ccggacgcgc aatacctggc tgtctattgc ttcgatgaga
    17101 caggaacgat cgaaggtggc tggatagagg tgcgctccct ggccgatggc cgggtgctgg
    17161 cccggcagga ccagccttac ggcgatgcgg gctggagcaa tgacggacag tccgtggtca
    17221 tgtttcatta tcccacaggc tacctcctgc ggcacagcct cgacgatgcg tcctcccagc
    17281 ctctggcctt gctgcgcacg gtgggcaccg gacggcgcgc tgccttccgc tcgggcgacc
    17341 ggcttctcgg ctggtcggac aatgaaaatt tcgtccacct gcatgacctg tgggtggggc
    17401 agcccacgct tgtgattccg ggcacggcga tgaacctcaa gttctccgcc gatggcgcac
    17461 gtctggcctg gaatcccgac agcgaaagcg ccgccatcgc ccaggtgctt gatccgcccg
    17521 tccttgaaca gcgcaaagcg gtcgggcatg agcgcagcgt ggaccgccat ctcgccgtga
    17581 gtcccgacgg gcgctggatc gccacgtgtg accttttcgg cctcaccctt tggcgcgcgg
    17641 cagacctcca gccggtggcc tggcgcttcg cgcaaagacc gcgtgatccc tttgaaagcc
    17701 tgcacttcag ctccgactcc cgccacatcc gcttctactg cggcacgagc aagcacgtga
    17761 actgggacgt catcgaggct gccgatggca ccatgactct gagcggcccc ggccccgaag
    17821 atgtgccctc acctcattac ctccaggctg ccagtccaga cgggcgctgg cagatcacct
    17881 tccgcgccga tgagaaaaat gccatctctc acctgtggga ggacgggaaa atcgtctcca
    17941 aacgccagcg cgggcctgag ccgggcctga ccagccgccg caactgcctc gtcagcccgg
    18001 atggccggtg gtgggcggca ggcgcatgga accgcgaccg ctcccgcctg ccgggattca
    18061 tctatgtgca aagcaccgag gtggacgact cccgcatcct cctcagcgaa gccgcctgcc
    18121 acacttatct cgccgtttct ccggatggga gatggcttct tggtggcgaa ggcgaaaact
    18181 acgccatttg ggagagcggc acttggaaac aagtcttcac cctgcccgcc gggctgtcgg
    18241 atgccgtccc cggcagcgcc gccttcagcc cggatggcag gctgctcgcc ctggaggtgg
    18301 atcacggcaa aatccgcctg ctgcgcgtcg gcacttggga cgaggtgctc accatcaccc
    18361 cgccacagga tctccccctc gtgctcatgg ccttctcccc cgatggtcgc cacctctaca
    18421 ccacgggcgg gcaaattttg caccggtggg acgtggcgcg gctgcgcgag gagctggagc
    18481 gtatgggatt ggggtggtga ggcgtgactg taggataagc gccccgcgtg cctgtttcac
    18541 gccccgtttg ccggaccccg acagccgggg caactgccct acaaccgcgt catgcacttt
    18601 tttttgccag ccggtaccct ttctcctccg gtgcggaaga agaaggggta tgcactcctt
    18661 ccgttccgcc atcgcccctg cagccatgtt cgccaggctg acgtccgccg tgctggtcgc
    18721 gcttctgccc gccgccacct tcgccgccga ccacacttcc acctggaacg gcagttcggg
    18781 gaactggacg gacaccacca ggtggaccac ccccactgcg cccggcacct ttcccaacaa
    18841 tggcagtcct actccgacct acgatgccat cctgaacggc ggggtaatca ccgtgaacca
    18901 ggatgtcgtc atccagaaac tcactcagag tggcggcacg aattcgggaa cgggattcgc
    18961 tctcaccacg aacgaactgc tcacgctgag tggcggagtg gtaagcggta cagggaccat
    19021 ccatgtcctc ggtggcgcgg tcattgggcc tgtcgagatg tctgggcgca ccctcaattt
    19081 cggcgacggc tccggcccgg tggtgaacac cacctggagt tcgggtgatc cgctggttct
    19141 tggcggtctg gtgatcaata acaggagcga ctcgaacttc atcatcagct tcgatggcgg
    19201 ctccttccga aacatcaacc gcagcggctc cgtggtcttc agcaaccaag gcacgtttac
    19261 caagaacggc ggagccggga cggccaacat cggggaaacg aacaacgggt cgtcgtcctt
    19321 tgccttcaat aacagcggca cggtcgtggt gaataccggc aaactcgtca ttggaagcaa
    19381 tggtggctat cccaacaccc acaccagcac gggcaccttc caggttgccg atgttgccac
    19441 gctggagctt gcgggcagtc ataccctctc caccaacagc cttttcaccg gtgcggggac
    19501 ggtgaacttc tcaggcggtg tcaccgccac tgcggcgacg acggtgaaca ccagcctcac
    19561 cctctccggc tctctgggtg gctccggccc cgtaaccgtc aacggaaacc tgctcctgac
    19621 aggatccatg aacggtggca acgccacggc cctcggggat gtgaccatcg ccatggcgtc
    19681 gcaaagagtc atcagcggag gagcggtgct caatctcgga gacggagcag gcccggatga
    19741 gggcagcacg accgcttggt cgagcggcaa catcgacctg caaagcaacg ggacggtcaa
    19801 caaccgcagt gattcggtca tcaacgccac ggcgaacggg accatgtcca acacccacaa
    19861 cagctcgacc accgccttca acaaccaggg gacattcaac cagaatggca ccggcaccac
    19921 gacgttgggg gcggggcagg gccccttcgt gtttcagaac agcggcctgg tgcaggtgaa
    19981 ctccggcacc ctggtcattg ccggcaccac ggggaccaac aatacgggca gctttgccgt
    20041 cgccggcggt gccacgctga acctgggcgg cagcatgaac ctgaatgcag gcaccacctt
    20101 cacgggcgcg ggcacggtgg tgttcagtgg cggcatcact atgaatacgc cgattacggt
    20161 gaacccccat ctcaccatca ccggcacgct gggcggcacc agcccggtga cggctgcagg
    20221 caacctgact ttcagtggtg ggatgatcaa tgatgggggg aacctcacgg ccttggggag
    20281 tgtgctgctc aacggggggg acagacagat cggcaatgcg gtgctgaacc tcggtgatgg
    20341 cgcaggccct gacgcgggtt ccaccaccac ctggtcggca ggcaatttca cattcagtgg
    20401 aagggggggc gtggcgctca acaaccggag tgattcggtg ctgaccacca ccttcgacgg
    20461 caagtttacc cagatctaca acggggaccc tgccagctcc gtcatcaaca atgcaggcgt
    20521 gttcaccaag agtggcggtg caggatccac tgtcatagga gacggcttct atgcggtgac
    20581 gttcaccaac tccggcacgg tgaacgcgaa cagcggaacg ataaggttca accacggcta
    20641 cacccagacc gccggagcac tggtcctcaa tggtggcacg gtgaaccgca atggcggcaa
    20701 ttttgacatc cgcggaggca agctgcaagg gggcggcacg gtgacgggtg gcgtgacggc
    20761 caccgggggc accatccaca tcgagccagg catcggcact actccgggcg cgctcaccat
    20821 caccggcgat ctcaccctga ccagcggctc caagctgagc ttcgacatcg gcggcaccac
    20881 gcccggcacg gagcatgaca agatcacgga gggcggttcg ggggctccaa acgtcaccgg
    20941 tgtgacgctg gaggtaaatc tgctcaatgg attcatcccg gcggctggga ccgagctcat
    21001 cgtgctggac gcttcccaca ccatcagcgg cagctttgcg aatgtggcca acggcgctcg
    21061 tctccctctg ccgggcggac tttactcctg caaggtgagc tatggtgcca gcagttcatt
    21121 tgcggacaag acacaggtga tcctgtccga gtttgccacc accccgacca gcctggaact
    21181 ggatgacaac gaaatcgtgg aaaacgccac cggaggcagc accgtgggcc tgctcagcgc
    21241 caccgatccc gacacgaacg aaaccttcag ctttacgctt gtcagcggca cgggcagcac
    21301 ggacaacggg tttttccaga tcactggcaa ccagttgcag acgaaggcgc tgcccacccc
    21361 cacggtcgtg gactttgaaa ccaagacctc ctacagcgtg cggctgcgcg tgaccaacag
    21421 ccgcggcggt ttcctggagc agaacttcac cctccaggtg ctggatgtgc ccgcacccag
    21481 cagcctggat ctcgacaccc tctctgtgcc ggagaaccag ccgctgaaca cggtgattgg
    21541 cactttgaca gccaccgatc cgatgcccgg tgccaccttc acctacgaac tgccagacag
    21601 cgcggaccgg atcagcctca acatcctgga cgacaaattg cgcagcagca aggtcttcga
    21661 caaggaaggc agtggagggt tccgtggtcc gttcaccgcg cgcgttcgcg tgaccagcag
    21721 cagcggcgag tttttggagc ggactttcac catcacggtg gacgatcagg gcccctcgaa
    21781 catcacgctt tccccagcct tcattcccga agggcggcct gccggcacgc cggtgggcac
    21841 actctctgcc acagtcgtgc cagagggcac aggtgtgacc tactccctgg tgggcggcat
    21901 tggcagcacg cacaatgcgc agttcatggt ggaaggggcg ttgatcaaga ccaccgcaga
    21961 gctccatgtg gcggatggca ccgaacgcag catccgggtg cgtgccacgg acgcgaacgg
    22021 ggagatcgcc gagcagattt tgaccatcac ggtgcaggtg gtgcaggtca ccgacatcct
    22081 gctggctccg ccgtcggtga aggaacggct gccagcaggc acggaggtgg gtcttctcac
    22141 tgccgtggcc acgccggagg ggctgccggc cacatttgag ctggtggccg gagatgggga
    22201 tgacgacaat ctgctctttg ccatcaatgg cggtgtgctg gagacgactg tcgaactgga
    22261 tgaggaggag caggacatgc ggaacgtgcg cgtgcgcacg acggtcagcg gcatcagttt
    22321 tgaaaaagcg ctggtgatcc aggtgctgga caatgatccc aatctggtgc tggagcaccc
    22381 cgagggcacg gtgctggctg tcttccctgc gggggatgcg gtgcagttcc cggtgacgga
    22441 ggcagggatg acctcggagc cgaaggtgtt caccatccgc aacaccggca cggggcctct
    22501 gaaagacatc gagatcccgg ggatgaatca tttcacggtg gatgtgacgg gcatggcgga
    22561 agaggtcgcg cctggggaga cgacgacgtt cactgtcagt ttcagtccgc agactccggc
    22621 ggccaccacc ggccctgaga gcgcggtggg agagaacatg ctcatcctca gcaatgatcc
    22681 cgacagcggc atgggcgggg atgagttcag cctgagcggc cgcgcgatga cggcagccac
    22741 cctggccatc gaacagccgg caggcaccgt gatcaatgac ggcagcagcc tctactttgg
    22801 cctgggcttg atcggcgcgg acggcgcgcc gcgcaccttc acgctgacca actctgggga
    22861 ggcgccggtg cggggcctgg ccgtgtcgct gcaaggcgtg catgaaggct ccttcgtgct
    22921 ggatgccgct ggcgtgcctg acgtgctgcc aggcggcggc agcaccacgt tcaccgtgct
    22981 catgcatccc gaggaggagg gcgcgctcac tgccaccttg cgcatccgtt ccgattcttt
    23041 cggcgacacc gacatcaacc tcaatggcga gggtctgaca ccggagcaga tcgcgcatca
    23101 ggcttatgtg aaagcgagca acaccgactc gggtgatcag tttggttatt ccgtggcggt
    23161 ggacggggac acggtggtga tcggggctcg agcggaatcc agcaatgcac gcggaatcaa
    23221 tggcgaccag ggcaatgaca gttcaggcct ctcaggggct gcctatgtgc tggtgcgcac
    23281 gggtggtgcg tgggtgcagc aggcttatct gaaagcctcc aacaccgaca gtggagacga
    23341 ttttggagtg agtgtggcca ttgagggtga cacgatcgtg gtgggtgcgg ctggtgaaag
    23401 gagcgcagcc actggtgtga atggcaatga ggcggataac accgcgtttc aagctggagc
    23461 agcctatgtc ttcatccggc agaacggcgt gtggagccag caggcttacc tcaaagcatc
    23521 agacacgtct gcaagtgctc atttcggctt tgccgtggcg atttctggcg agacggtcgc
    23581 tgtcggtgcg catggagcgg cgagctttgg ctcaagcacc ggtgcggtgt atgttttcac
    23641 caggagcgga gcagtctgga gccaggaggg aggaccgctc aggccgggct catccggtga
    23701 tggcaatgat tttggctggt ccatcgcttt ggatggtgat ctgctcgtgg ccggcgcgcc
    23761 gggcgatgac agttcgattc cagggatcaa tcagggttct gacgatgacg cggaggatgc
    23821 gggcgctgcc tatgtgtttc agagaagcgg cggcaactgg ggcgagcagg cgatgctgaa
    23881 ggcgtccaat gccgaaagcg gggacgggtt cggcaggagc gtcgccatct ccggtggcat
    23941 cgtggtggtg ggagcattca atgaggatag tgccgcgcgt ggtgtgaatg gggatcaggc
    24001 cagcaacggc gcacctgact cgggggcggc gtatgtgttc gcgctcgatg gtttcaactg
    24061 gcagcaggaa gcttacctca aagccagtaa cacgggagat gctgaccggt ttggatattc
    24121 cgtggccatc catagggaca ggctggcggt tggtgccttt cgtgaaggtg gctcgggcac
    24181 aggagttgat ccgctggacg acgatggcgt gaacagagcc ggggcagttt acctgttccg
    24241 ccgtgctcct tcctttgcac ctgaccaagc caccaatttc tcctgggttc agaacagcta
    24301 cttgaaggct tccaacaccg atgcgttcga ttcctttggc ctctctctgg ccttgtctga
    24361 agagcatttg atcgttggtg ccccccaaga ggacagtgcc gccacgggtg tggatggcga
    24421 tggcagtgac aacagcgcca ctattgccgg ggcggcttac atcttcgccc tcgagtcacg
    24481 ggcgatccag gtgtatgcgg gagccgagct aacagcagac cgcgtgcaca gcggccaggc
    24541 cgcgccggtg gacctgggcg gctcgccgcc ggcggtgccg ctgacgcgca ccttcaccat
    24601 cctcaatgac gggctgctgc cgctggacat cagcgcggtc catgtgagcg gggacttctt
    24661 cgtggtggat cagggtgatc cggcgctgcc gggctttggc gccatgcagt tcacggtgca
    24721 gaacgagccc ggcacttccg ggccgctgaa tggcacggtg cagatcgaaa gcgacgaccc
    24781 ggacctgccg cttttcacct tcaccctcgc aggactggtg ggcgatgcgc cgcccacgga
    24841 gatcgccatc gaacaaccag agggcgcgaa cctgaccgac ggcgcgagca cggtgaacta
    24901 cggcgcgctg acctacagcc gcaccgccgc gcgcgccttc accgtgcgca acctgggcac
    24961 ccagccgctc tacatcggag caatcacgat cacgggagct gacatggcgg actttgccgc
    25021 cgggccgccg gggcagtccg aggtgcagcc tgggcaggcg acgagcttca tggtgacctt
    25081 tgatccaaca ggcacggtgg gcggcagcag gacggcggtg ctgcatgtgt tcagcaatga
    25141 caccgacgag ggttcctttg acatcaccct gaccggaacc gtgcaggtgc ctgacattga
    25201 ggtgaccggc gggacgatcg tcggcggcgt gccgacgctg gactttggga cagtggtctt
    25261 cggcccgggc aagccgggca gggatctcgc gataggcgtg ttcaatgtgg gccagggtgt
    25321 tcttgaggac ttcaacttca acatcgaggg ggtccacgcg tctgatttca gcatcatcaa
    25381 cagcgccatt ttcaccagtt tcgggggtgc ggcgcagttg gcggtgcgct tcctgccgca
    25441 ggaggagggg ccgcgcagcg cggtgctgcg ggtggagagc aatgatcccg atgaaaaccc
    25501 gctgctcatc aacctggccg gcaacggcaa tgccctgccg gtgatcaccc agcagccgca
    25561 gtccgtgctg gtcttcacgg gcgagtccgt gaccttcacc gccgccgcaa cgggcagcgg
    25621 gttgacctac cagtggctga aggacgggat tcccttcggc agcgccaccc ccagcctgac
    25681 gattcccaac gtgctcgcca cctccaaagg cacctattcc tgccgggtga gcaactccgc
    25741 cggagtcact ttttccagcg tggcgcattt ggcggtgatg gcttcctcac cagccccatt
    25801 tccagttcag gccaatgaag acacgaccaa tattctctca atggcatccg tctcgcctcc
    25861 tgcccagacg aaaatcctct accactggca gaaagacggg gctgacctca gcgatggcgg
    25921 cacagcgcca gtgatctcag gcgccttcac cagaagtctg cgtctcacag gcgtcaagcc
    25981 ggcaagctcc ggcacttaca cctgcaaggt gacactcatt tcgtcgaacg gaggcggcaa
    26041 tttgacccgt gtgggccgga ccattgtgct cacagtgggt gccaagcccg tgatcgccgc
    26101 cggggggcct tttgagtggg gagcggccac gccggtttct gatgtgatca ctgtcactca
    26161 gaatccaggc ggtattcgct attccggtca tccgacaggt gttcgcctca gcattactgg
    26221 tgccggacaa cttacgttcc agggcaaacc cacgcgcata ggcagcggta ccatcaccgt
    26281 gaccgccagc aacgccgcgg gcaccagtct gccacagacc ttcaactaca ccgtcgccgc
    26341 gctgcctgcc aatgtggtgg gcaccttcga tgggctggtg gaccgcagct ccttgtttgg
    26401 cctgcacaac ctggggggca aaatctcggt gacggtgggc agcaacggct cctggagcgc
    26461 agcgttgctg ctgggaccca aggtttactc gttgacagca tcaccagcca cttcgaacac
    26521 caggttttct ggaggcatct tcagtggcag agcggtggtg aaccggtcga gtgagggact
    26581 gccgtccttg aacctggaca ttgagattga tgccagcaca gggaacctga ccggcacgct
    26641 cacagacgcg gccctgacat cgccacagcc aggagaagtg gcactggaag cattcaaggg
    26701 cagtgtgctg ccgcaattca gctacactgc ggcgttggaa gtgtcatctg ctctggccgg
    26761 gaaccccagc gtgcctcagg gcaacggttt cttcacggcc aaggcaggct ccaattcgac
    26821 gctgaccatg gccttcaagc tggcggactc aacggtgaaa acctacgctg gcccagtcgt
    26881 cgccggtgcc cggattccgg tcttcttcag cctctatgga aacgtgccat cctctgctgg
    26941 ttcagtccat ggatggatca cccggactgc ggacaccgtg ccgcaggtca atggtggcag
    27001 gccgctgctg aacggcgcac tgacatggct gaagcatcct cagccctcca gcagcaccac
    27061 ccgcagctac aaggagggct ttcctttgca cacgttgacg gtgctgggtg gacagtatgc
    27121 gcccccgcct gcggggagcg tggtgctggg cctgcaggcc aaaaccagca atgcccgctt
    27181 tgcctttgcc gaaggcggga tcgagttttc cgccctgggt ggcagcggca gtccggcagg
    27241 cacacctgcg ggcatcatcc gccaagtctg caccatcagc accagcaacc ttttgactct
    27301 gcctgccatc aacaccgcgc agaacccggc caatgtgcgc gtcaattcgt ttgatgccgc
    27361 caccggtctg ttccgcggca cgttcatcct gcgcgacaat gatccgacgg acctgctgcc
    27421 gccgatcacc cagttgagcc gcaacaccgc cttcgaaggc gtgctggtgc cgcgtctgga
    27481 caggggagcc gggttcttca acctgatcct actgcccgtg cccggccgca cgttgtccac
    27541 gatgccaatg ctttccgggc aagtgatcct ggaggcaaac ccgtgagtct tgcctggagt
    27601 gaatggccgc ggaagttcca gaggggcgca tggccaccgc gcccctggct tgcggccatt
    27661 ccagcatcaa acgggcgatt tgcccgcgcc accgcgccgc aggccgtgga ccaggaacgc
    27721 ggctccgacg acgatcatga agaccgagaa aaactggccg cgtgtgaggc cgaggatggg
    27781 gtttgcgccg gaatccggct ggcgcacgca ttccaggctg atgcgcgcgg cggcgtaaag
    27841 caggaaaaac aggccggtga gcacaccgtg gccaagcctg ggaaagcgga tgcgaatgaa
    27901 caaaagcacg gcgctgagca gcagcccctc ccccagcgcc tcgtagagct gcgagggatg
    27961 ccgggggtgc agcatggcct ccaactccgc cactccgccg ggctggccct ggaaataggc
    28021 gatgatgtca gggctgtgca cgactcccgg cggcagagca acgggcgcgc ctccctgcgc
    28081 cgcgaaatcc tcgtgcagca actcggcggg aaatttcacc gccagaggaa aggcggtcac
    28141 acggccgaaa agctcgccat tgatgaagtt tgcgatgcga ccgcagaaaa tgcccagtgg
    28201 cgcgccgcaa accagggtgt cgccgatgcc ggtccatgag attttgtgtt tgcgcgcata
    28261 ccaaaggcag aacaaggaca ctcccgcgat gccgccatgg ctggccatgc cgccctggtt
    28321 gagcaggaag aaactccacg gcgcgcggat gaaattctcg aagtcgtaga gcagcatgta
    28381 gccgagccgc ccgccgagca cgacgccaaa cagcgccacg agagtgatga aatccgccac
    28441 ctgggccggc ttgatctggg acaggccgcg ccgcgccagg aagaccatga cgaggtaggt
    28501 cagataaaac ccaagcacat aagccaggcc ataccagtgg atggcgatgc cgtcggtgat
    28561 tctcagaaga tgcgggctga gatcgtgcaa gtaataggcc aggagattca tgcggggcca
    28621 agcattggcg gcgggagccg gacggggcaa gcgaaagcgc ctcacgcctt cgccacaatg
    28681 acggggccgt tgtttttcgc cgggttgtcc ggcgatccgg tcactgtggg cgcgtccggc
    28741 tttgcatcag tctccggcgc tccggggacg ccggcttcca cgatttgggc gagtgtggtt
    28801 tttctcgccg cgggcgcctc cttcgcgatc tcaggttcag gtgtttcagg gcggcgctgt
    28861 tccaggtcgg ccacgatcac ggccacaagg caggccgccc atgcggccag cacgccggaa
    28921 aagggttcga acaaaatccc cgcccatgcc agcgtgaagg cgaggcagac cgtgaggtga
    28981 gtcacggcga gcagatagat catctttagc cacaagcctc cgcgcgcaag accggtccgc
    29041 gcgctgagaa aagccagcgc ggtgaagcat gcatactgca gcggcaatga catgcgcagg
    29101 ccgtgctccg gcagcaccag cagacgcggc agacgctcct caagccacaa ggccatctct
    29161 ggaaagagtc cgaaacggtg gagcgcgagc acaggcacgc agaccaggat gccggtgaag
    29221 aggatggaga cgcgctgttg ttcgttggcc ggggtcatga ttcgggagag ggggctggct
    29281 cggcgccacg gtaacagttc accacgaggt cgctgagaaa tttgtcgggg cgttccacgg
    29341 cttcgggacc aaggtggaag accttccgcg ccgtgttgcg cgccacgagc tgcaggccga
    29401 aggggaagtc tttgaaaagc ctggagcaca actcctccac gccgctgccc tcacgctccg
    29461 cgcggcgcac caggccttgc acggcgccgg cgtcgaacac cagctccagc ccatgctccc
    29521 gggtgaagcg cgcggcaaag gcgcgcacct cccccgcacg ggcgtcggtg cgcagactga
    29581 gggccgcctc gcggctgtgt tcgagagcgg cggagggatt cagcaccagc gccttgtcca
    29641 cgatcaactc cggaagcccg aggctgggca tttcaaactt gaactcgcgc agcacttttt
    29701 cccaggctgt gacgaggccc cgcgcgccgg ttttctcccc ggcggcgcgg gtggccacct
    29761 cgcggagggc gtcatcctcg aaaagcgcgc gcacaccata agcgtggaat tcgcgctcga
    29821 actggcggat gagactgccc tcgctgtttt tcatgatgtg aaaaagatca tccgcgccaa
    29881 gcgcgtcaca aaaaacccga acgggcaggc gcccgatgaa ctcggcctcg aagccgtagt
    29941 ctatgaagtc cctggtcccc gcctcgcgca gcaggtcctc ggcggcgggt tttccgccaa
    30001 ctgacgcgcc gaagccgatg gaggcgcggc tctggcgttt ttcgatgatt ttttccaggc
    30061 cggaaaaagc gccgctgaca atgaaaagga tgttgcgcgt gttcaccacc tcgcggccgg
    30121 tgcggccctt gcgcgtgtcg aacatcatct gcatctgtcc gcggatgtcg ttgggagcat
    30181 aaagcggaac ctcggtctcc tccatgagct tgagcagggc ggtctgcaca ccgcggccgc
    30241 tgacatcccg accgatgcgc ccctcgccgc cggtggccag tttgtctatt tcatcgaggt
    30301 aaatgatgcc gtgccctgcc agccgcacat caccgccggc ctttgccaca agatcgcgca
    30361 ccaggtcgtc cacatccgcc cccacatagc cggtctcgct gaacttcgtc gcgtcggcct
    30421 tcacaaacgg cacgccgatc aggtctgcga tgtgtttgac gaggtaagtc ttgcccacgc
    30481 cagtcggccc ggcaatgagc acattttgct tggagaactc cagcgccgga ccgtcgccgc
    30541 cgcgctgctt gcgctcctcg gaaatcatgc gcgcgtggtt gtagtggtcg cacacagcgg
    30601 tggccagcac ctttttcgcc tcgtcctggc ggatgacaaa gcggtccaga tatgctttga
    30661 tgtccgcagg tttgagcctg aaatcgaact caggttcggc ctcggcctcc tcgacaggtt
    30721 ccgggtcgcc gcgatgcggg cgcagtccgg gcatggacgg ctcgaccctg gaaaagagaa
    30781 cctggccgcc aagggtgttt ttgatgaagt cctccagctt gcgcgtgatc tcctccggct
    30841 tggctggcgg ctgggccggc ggttcatccg gcttgttctc aatgtcttcc ttcatggcgg
    30901 ggactttcgt ccgcaaaggc tgtgccgcaa gcggtcatgg cggcgggttc gacttgtgat
    30961 tttcgcggga cttgggcaga aaaactgctt ttctgacgca gcccaatgtc cgcgccgccc
    31021 cagtccatcc tcagcgtgtt caaccgctac cggcatcccg gcggcgagga gatgtccgtg
    31081 gagcgcatcg ccgggctgct atccacgcgg caccatgtcg ccaactgccg ttttgaaagc
    31141 agcgaatgga ccggcccggg cgcgccttcc acccttggcc aggcatggcg gttgttttac
    31201 aatccggagg gagtgcggcg attcgaaagc gcgctggaca aatccggagc ggccctggcc
    31261 ttgctgcaca acatttttcc agtgggttct ccggccatct accgcagcgc cagaagacgc
    31321 gggctgccgc tgatgcagtt catgcacaac taccgccctt tttcagtcgg tggaacgctt
    31381 tactcccgcg gacgcatcct caaggacccg ctcttcggcc attattggag ggaggtagc
//
LOCUS       DAOMYJ010000033        31378 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Prosthecobacter sp. isolate AMR_MDS_5629
            NODE_522_length_31378_cov_22.673531, whole genome shotgun sequence.
ACCESSION   DAOMYJ010000033 DAOMYJ010000000
VERSION     DAOMYJ010000033.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867850
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prosthecobacter sp. (wastewater metagenome)
  ORGANISM  Prosthecobacter sp.
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Verrucomicrobiaceae; Prosthecobacter.
REFERENCE   1  (bases 1 to 31378)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 31378)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.30
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:34:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,742
            CDSs (total)                      :: 4,684
            Genes (coding)                    :: 4,676
            CDSs (with protein)               :: 4,676
            Genes (RNA)                       :: 58
            tRNAs                             :: 55
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31378
                     /organism="Prosthecobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_522_length_31378_cov_22.673531"
                     /isolate="AMR_MDS_5629"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1965333"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            224..1216
                     /locus_tag="PLP58_05490"
     CDS             224..1216
                     /locus_tag="PLP58_05490"
                     /inference="COORDINATES: protein motif:HMM:NF024810.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kelch repeat-containing protein"
                     /protein_id="HRJ08001.1"
                     /translation="MHTRTILMAGGLIILMAVPCPAAEWEKLPPLPEPNGGFACAALG
                     EVIVVAGGTNWEGGVKNWLDAIHIFSPKTQSWRQAGRLPSPQAYAVSGVVEDENGWPQ
                     LVIKGGTMGERTVPEWRSLNHGGGQTVMDIAPHPRVLAAGGIVNGWLVQVGGTNDPAN
                     LAGLVRAALLLKPGDGLIDILPDYPGAPFGTAACTVAAAELFIFGGANWDASANTVRN
                     AVEAHALHVAKRAWRPLKHLPCPVRGLAAVDLGNGSLYLAGGYKSDTEGFTAEAWFYD
                     IAKDEYKSAKPLPYSGMVNLVVCKGHLYCLGGEDRQKSRTNACYRIGLPELEVK"
     gene            1438..2097
                     /locus_tag="PLP58_05495"
     CDS             1438..2097
                     /locus_tag="PLP58_05495"
                     /inference="COORDINATES: protein motif:HMM:TIGR03901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MYXO-CTERM sorting domain-containing protein"
                     /protein_id="HRJ08002.1"
                     /translation="MIVCAGPSRANLGTFYFTPFEFAYAGGLSSPALEGSLFALEVHE
                     VFGNPNSVYLSLLNLSDLSHPNISATSPTITGIYFDDNASLLNFGSASIFDQSPMTGF
                     NLGGGGPPNVPGGVGISFFSDYRVSATPPSPSMGLDPGQFLTVQFGLLGGATASDVFQ
                     ALAHEEASSGLRLALHVQEIGVGSSTEASGSFVTVVPEPGGFLLLAVAGMVLQLRRRR
                     R"
     gene            2171..4156
                     /gene="thrS"
                     /locus_tag="PLP58_05500"
     CDS             2171..4156
                     /gene="thrS"
                     /locus_tag="PLP58_05500"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009959529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004829 - threonine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006435 - threonyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HRJ08003.1"
                     /translation="MRQLGRPFAMANERKTLEERALMSDIERLRHSCAHVMATAILRL
                     WPDAQFAYGPPVETGFYYDFQMAHRITPDDFEKIEAEMKKIAKENQRFERRVISREEA
                     IALAKSGRLGGLIERPGNESRFKLDLIEKIPEGEEISCYQNGDFLDLCAGPHVGYTSK
                     CKNVKLTAVSASFYMGDESKGQLQRLYGTAFESKEEIEAYFVRLEEAKKRDHRRLGKE
                     LKLFHIDEDVGQGLILWTPNGAVIRQELQNFISEELRKQGYHQVFTPHIGKLALYKTS
                     GHFPYYKESQFPAIVEQDQLEQIAHEGCTCAEMTARLDAVSAQFASQINERTGTEVIG
                     QDRVLDAEKLLDGFLLKPMNCPHHIKIFASQPRSYRDLPVRLAEFGTVYRWEQSGELN
                     GMTRVRGFTQDDAHLFCTEEQVAAEVLGCLSLVKTVLTTLGMEDYRVRVGLRDPDSAK
                     YAGRPEQWDRAETACREAAQSLGVPFTEEPGEAAFYGPKIDFVIKDVIGREWQLGTVQ
                     VDYQLPIRFDLSYNGADNKPHCPVMIHRAPFGSMERFCGVLIEHFAGHFPAWLAPEQV
                     RVLTISEKVDAFASEVFAQLKAAGLRATLNNEADKIGAKIRNAQLDKVPYMLVLGEKE
                     AAAASVAVRHSKKGDLGVKPVADFLTEITAEVRERRL"
     gene            complement(4360..4803)
                     /gene="nusB"
                     /locus_tag="PLP58_05505"
     CDS             complement(4360..4803)
                     /gene="nusB"
                     /locus_tag="PLP58_05505"
                     /inference="COORDINATES: protein motif:HMM:TIGR01951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HRJ08004.1"
                     /translation="MGKRREGRAAAVQYLFARELQGEGEPANAGEFWSIHSAKASVRS
                     HAESLAQGVLSHLDEIDAHIRPVLDNFRIERLAAVDRNILRLAVHELLHLPDVPGPVV
                     INEAIEIAKSLGGGESGSFVNGILHKIAQKVRKPVAAEAPGREGG"
     gene            complement(4809..5315)
                     /gene="ribH"
                     /locus_tag="PLP58_05510"
     CDS             complement(4809..5315)
                     /gene="ribH"
                     /locus_tag="PLP58_05510"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009962131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009349 - riboflavin synthase complex
                     [Evidence IEA];
                     GO_function: GO:0000906 - 6,7-dimethyl-8-ribityllumazine
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HRJ08005.1"
                     /translation="MSQHGPSRPRTIHEPVSIAIVASLYNNTYVQGLLDAGCEEINEI
                     APNASITVYRVPGAFEIPVCAEMVLRHSLPDVLIAFGVIIRGATEHGDLVGASVTDAL
                     QRMAVAHCTPVVHEVLLVSSEEQARERCLGESINRGTEAGRVAVQMISLFNKMRASFA
                     GETGLEDA"
     gene            complement(5377..6591)
                     /locus_tag="PLP58_05515"
     CDS             complement(5377..6591)
                     /locus_tag="PLP58_05515"
                     /EC_number="3.5.4.25"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007418866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003935 - GTP cyclohydrolase II activity
                     [Evidence IEA];
                     GO_function: GO:0008686 -
                     3,4-dihydroxy-2-butanone-4-phosphate synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     3,4-dihydroxy-2-butanone-4-phosphate synthase/GTP
                     cyclohydrolase II"
                     /protein_id="HRJ08006.1"
                     /translation="MPAKKKHVCDPMESVIADIRAGRMVIITDDEDRENEGDLICAAE
                     KITPEMVTFMVREGGGMLCVPVSLEIAHRLGLESMVPENREAFRTDFTVTVDAAKGIS
                     TGISAADRARTIRLLAAPESSTQDFVQPGHINPLVAKPGGVLRRAGHTEAATDLCRLA
                     GLREAGVLIEVMNPDGTMARLPDLKKFARKHKLKMGSIADLIGHRRRSEKLVEKIEDV
                     DMPTDFGTFRLHLYKSALDGVHHVALVMGKIDPAKPALVRVHSECLTGDIFTSRRCDC
                     GSQLHSAMRMVAEAGAGVIVYMRGHEGRGIGLHGKILAYKLQEQGLDTVEANLKLGFP
                     MDLRDYGIGAQIISDLGVRKIRLMTNNPRKVVGLEGHKLEIVEQVPVISDPNPHNKKY
                     LETKKKKLGHRL"
     gene            complement(6725..7606)
                     /locus_tag="PLP58_05520"
     CDS             complement(6725..7606)
                     /locus_tag="PLP58_05520"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HRJ08007.1"
                     /translation="MLSSTVTASAALLDHLFCLWPEVKRTKMRQWLRFGAVHVNDRPV
                     TRHDHPLQPGDVITIRPRKAPPQVAPLPAGLHLLHEDESILVILKPAGLLTVATDTER
                     QRTAFRFLTDHLRERTGDARARLWIVHRLDRDTSGLIVLARTEEAKLALQESWDLAEK
                     QYQAVVEGALEQKQGTLRGHLDESQPHRVYAAARPGPSTREAITHYRVVREGYGRSLL
                     DITLETGRRHQIRVQLAAAGHPIVGDVTYGGKPASSTRRLALHATALRFQHPATGREM
                     CFQSPLPEDLARLVPGR"
     gene            7988..8626
                     /locus_tag="PLP58_05525"
     CDS             7988..8626
                     /locus_tag="PLP58_05525"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HRJ08008.1"
                     /translation="MKHKIYLLDDHPIVIEGLKKIFEGQEDLQFVGYSEDANNALVEI
                     EKLKPNLVILDLTLRDRSGVDLIKKLKQSHPSISVLVYSMHDENVYAERCLRAGAMGF
                     IMKGEPAERVIQAIRQVLGGGFFFSAALLSSIVSGGGPRSGSRGEPGRMLSDRQLEVF
                     EMVGRGFDSHEIGEKLQLSAKTVDAHKANIRQKLGLDSARELLMEAVRWVEK"
     gene            complement(8693..9940)
                     /locus_tag="PLP58_05530"
     CDS             complement(8693..9940)
                     /locus_tag="PLP58_05530"
                     /EC_number="1.17.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009964155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-enyl diphosphate
                     reductase"
                     /protein_id="HRJ08009.1"
                     /translation="MSAAPAPAGSRRVNVRTPEVMERVQAEVESHYRSPLVEAVRASG
                     GVLTIDGTTVRLAREFGFCYGVERAIDLAYAARKVFAERRVFLLGEIIHNPEVNRQLT
                     EMGIVSIPVGRHEQAIADFQSEDVVIVPAFGAETRLMQKIAERGCMVVDTTCGDVMSV
                     WKRVRNYARTGFTSIIHGKAEHEETRATSSRALGDDGRGHYLVVLTLADVDFVCDHIR
                     NGGDKAAFLDRFKGASALSEGFDPDTHLRRIGVANQTTMLKSETEEIQRRLHQAIQDR
                     DGPEEGAKNFLVFDTICGATQERQDSLFHLLRHPLDVLLVVGGYNSSNTTHLVEIGEQ
                     QLPTFFIRTAECLESLEQIVHFDLHSKSEKTSYSGKLASPDPVVIGITAGASCPNNLI
                     EDTLLRVFQLRGISPDVVRAAVL"
     gene            complement(9946..10575)
                     /locus_tag="PLP58_05535"
     CDS             complement(9946..10575)
                     /locus_tag="PLP58_05535"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015679.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_function: GO:0071972 - peptidoglycan L,D-transpeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HRJ08010.1"
                     /translation="MKSSPVPHVLRFLVLAVLAAGLASCVTKKPRGGNYSTRFDPPME
                     AVKNPSALKLKISTGAQRIYLVEGDRVLLASPCAVGTASSPTPAGHHRITGKTAQRRR
                     VSSPGAGYPMTYWMSFYAPAYGMHWGWVKPYPCTHGCVRLPIKTAKKLFDAVPVGTPL
                     HVAASHPEDATHGKSLPVLDDSTLPDPPDSYMLSSQVFTDAAQGKVYNY"
     gene            10691..11332
                     /locus_tag="PLP58_05540"
     CDS             10691..11332
                     /locus_tag="PLP58_05540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1080 domain-containing protein"
                     /protein_id="HRJ08011.1"
                     /translation="MNHQLLTALLFSLPLAAAAQTPDAEPKTEWKLFTGKSLDDWETV
                     DIGGSGEVTLEGGQMIIAQGDSLSGVVYKKVAELPLTNYEITLQAKRLQGVDFFCGLT
                     FPVGKLDRCATLILGGWGGSVTGISSIDDLDAANNSTGSYQRFDDDKWYSVRLRVTPE
                     NLSAWVDEKQVVDADINGRKVNLRPGPIEAYAPLSITTFTTMAAIRGVTLKKL"
     gene            complement(11345..15589)
                     /locus_tag="PLP58_05545"
     CDS             complement(11345..15589)
                     /locus_tag="PLP58_05545"
                     /inference="COORDINATES: protein motif:HMM:NF037016.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HRJ08012.1"
                     /translation="MKTILLGGALLLAAAQTHAQFLGDEDFSGAAKNESKWGADVSSG
                     SGQLAQTGGVLRFTSDGMTDDDFMAWPWLADAPFDSSWSVQVDVHVPHIALADGHTAA
                     GMGIIVRSGLDDTDAFSMALENYRNPGEAQQFHFFNAVDIDDDTTETFAEAVNHLATV
                     RISWNAVTRTLTAEYDADGPANGQAWTALQSFNPAGGGWNMTEGDVFQILIGGYSEET
                     EVTGVMNMHADNFSVSEMVLVNLFGGDNFNDNDRNATKWGPDDSGNTGALNEVNGRLE
                     YTGDGMASPFGYSERPWILNTGSYTQDWEARVDVHVGHVSLAEEGDRLQMVLAVYGGD
                     SVGEVQLEVEHVGGSIPQRRFRYTTEGDVGETHADTSSTDGSLRISFDAASKMLSFYY
                     DFNGPEGGHAWTLLKTVDVDAPATDWMMDASSRFTLAVFGEHQGMTVNSGDAYADNFE
                     AISAVQSVVAGSPTGKDDFEGASKNTSKWGGNISEGSGLLTQTGGVLRFTSAGSSDED
                     FMVWPWLVNAPFASDWSVQADVRVPWIPLSGSFTAVGAGVFVSHSADGRDRFSAWLEN
                     YRDFDSPQRFHFFNEVDFNDEDEPETFEDAPSTHAAVRISWDAGSKTLSAAYDPDGPL
                     NGYSWTVFRTFNPVTGGWGMTASGSFKIHLGAYSEETQVTDAHQVFMDNFHTFGILGK
                     PVPPVVKTLAATDATFEGATLRGTVDAKGFAREVFFDYGTTTALGMTVAADPATVDGS
                     GVENVSAVLSGLNPHTKYHYRARAAGVQVAANGATMSFTTANRAPAAVNDTFTVLPGS
                     TVVLDVFDNDFDPDGDALLLASFTKPGAGASLKKTGNTLVLTTAANFSTPTLFSYTVK
                     DAFGGSAFAAVTLMPGTLMLDLSEKSDLASVGGSHDIEVTSDGLWSVQTPPWIISGPP
                     AGMGDGTVTLTVLANTSLKQRTATVLIGGQAHTVTQAGVLKPALAMPAQIDSATVSGD
                     YVLQIPVVNLPVTFTIKNLPPGLTANGNTGVISGKPLKGGAYSLVIKAKNAAGAADET
                     LEFDINVGFLPDGIAGLFHGLIEPHPLLNGNLGSRYEMTVASTGSVSGKVITGTTALP
                     FKGQLDADVDDEDHPALTVMLQPKGADPQTLSLFFDADDNLVAGDLASGGNDTPVNAW
                     RNAWTKDDNAADFKGPHSFRLIQSDPAAAFPQGDGFGAFTVAEANGGLTVGGNLGDGM
                     AFATKTFIGRSGQVLVYHSLYAKKGSLAGVLEVTPGFEPVDNDVMGSLSWFKPGGDAK
                     SKDTLYQEGFGPLMLDAEGGGYPMIDKGGMLMGFDIGDDNALLDFSAGGLDDVGMEFD
                     LLVTLFNPSAAGVTLKARVPTALDINPNRVKISKLDTKTGQIAGDFLLATVPPRKAVW
                     QARLVRTGGSVQGHGYFLLPDGAVRKSGRVLLGAFP"
     gene            complement(15728..16366)
                     /locus_tag="PLP58_05550"
     CDS             complement(15728..16366)
                     /locus_tag="PLP58_05550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ08013.1"
                     /translation="MILNLLRELVPSPALEIIRLLKQSPGMTVNELKGALKMSYMGVK
                     QHCDALVKKGCLDTWRHPVPHGRPEKIYRLTQKLDPLFNGGAPDPLIEMLGHAERVFG
                     PTAAEKLLYAFYQERAARYQEAADKESLLENKAIVIARLRSQEGRLCEVEMSPGGDLR
                     LVDYHFPLVEVVKKYPLVEEIECEMIERVLGRPVGRTVEERSGLRRVIYLIG"
     gene            complement(16462..18780)
                     /gene="priA"
                     /locus_tag="PLP58_05555"
     CDS             complement(16462..18780)
                     /gene="priA"
                     /locus_tag="PLP58_05555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018970148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005658 - alpha DNA polymerase:primase
                     complex [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HRJ08014.1"
                     /translation="MPPPSDTPNLGLFGPEEGVSEPGGQKIARVQVETAAALELDYLI
                     PEKLADQIRLGSRVTVPMQNQRVRAVVIELPEQTVHGGRLKEIFALIGARPMFTSGLL
                     KLAHWIAGYYVVPVNRVLRTMLPQAVREKPETFLTDSHLKLAKEPPPEIFDKLHRTAP
                     MQARILEKLRSSGGESTLSELRRELPRATAIIKPLLAAGWITRSELRVERDPFQTEDF
                     LPSQALTLTDEQTAALAEVLKAVNHTGETAPRPLLLHGVTGSGKTEVYLQAIAEVLKQ
                     GRTALVLVPEISLTPQTIERFKARFSDKKDAVAVLHSHLSDGERHDEWFKIHEGRAEI
                     VIGARSAIFAPLENLGIIIVDEEHEPSYKQEDAPRYHARDVAVVRGHLERCAVLLGSA
                     TPCLESFQNARAGKYQLLRMTRRTDGKSMPLIRIVDMRLERRKGTEVSAANAAILSQR
                     LRAAITDRLEKREQTILFLNRRGYNTSLSCLACGEVVQCQDCAIPMTLHKKDNRLVCH
                     ICGARRVPPVKCPACKEPGLKYGGFGTERVEAAVREVFPQARSARVDTDTMQRKNQLR
                     DILRDFRAQKLDILIGTQMIAKGLDFPNVTLVGVLNADTALNLPDFRAAERTFQLLVQ
                     VAGRAGRGEVKGEVFVQTHAPHSPAIQFSRHNDYEGYADQELEQRKALAYPPFSHVVL
                     LSARGPHETQAEFTLQTLRKRLAEGLPEGAYLGHPAPAPLSRAHGQHRFQLLLSSPAI
                     RPLCAHLRAVVKATTLPADIIVTWDVDPMHLG"
     gene            complement(18858..20261)
                     /locus_tag="PLP58_05560"
     CDS             complement(18858..20261)
                     /locus_tag="PLP58_05560"
                     /inference="COORDINATES: protein motif:HMM:NF013741.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HRJ08015.1"
                     /translation="MNLLHHPIPVLALGGSHEVLHEWNLTAGEVAWSAALVLFFVLLN
                     AFFVAAEFAIVKVRGTQLDALADEGNRAAVTARAEIKNLDGYLSATQLGITIASIALG
                     MIGEPYLARIIQPLMWKLGITSDTVISSVSIGIGFGFITYLHVILGELMPKSLAIRKA
                     LPTTLAISRPLHFFYIVFKPAIWVLNGTANWLLKVLFRLEPASESELAHSEEELRHIV
                     AASSESDEVTDTEKDILLNTLALNDRCVRDVMTPRNQVVCLDAGDSFEASLAAAIQHK
                     HTRYPLVEGHLDQSIGLIHIKDLLGLVGKSGADLRGVKRDLPMVPEMMPIDKLLRFFL
                     DKHVHIALAVDEYGGTVGIVTLDNVIEEIVGDIQDEFDHETSEFRRVNDDEFVVEGTL
                     NLYELAEQAGINIESDEVTTIGGYVTHLLGHLPRIGETVVIESYDVITTRADARRVQQ
                     LHFKRRLARNEDGGDKE"
     gene            complement(20300..20551)
                     /locus_tag="PLP58_05565"
     CDS             complement(20300..20551)
                     /locus_tag="PLP58_05565"
                     /inference="COORDINATES: protein motif:HMM:TIGR02605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HRJ08016.1"
                     /translation="MPTYSYIAEDPEKGCPSCRRGFDLRRPMSRPALAHCPLCRQPVK
                     KLVSTFNTPVITRPLSVTDAKKAGFTILEKRCDGSYERL"
     gene            complement(20620..20889)
                     /gene="rpsN"
                     /locus_tag="PLP58_05570"
     CDS             complement(20620..20889)
                     /gene="rpsN"
                     /locus_tag="PLP58_05570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004924130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HRJ08017.1"
                     /translation="MAKTAWIEREKRKQKTVNKYAKLRAELKAQGDYVGLSMLPRDAS
                     PTRLTNRCRVSGRRRAYMRRFQMSRLTFRECALAGLIPGVTKSSW"
     gene            complement(21073..21663)
                     /locus_tag="PLP58_05575"
     CDS             complement(21073..21663)
                     /locus_tag="PLP58_05575"
                     /inference="COORDINATES: protein motif:HMM:NF012521.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HRJ08018.1"
                     /translation="MKYLTVIRHAKSSWEQSGLADHDRPLNERGLRAAPAVGSFLRHT
                     YFGGGETPPLLPSPDLLVTSTALRALTTAQIMREHFALPVEALRLDSRMYLAPAQRLL
                     EIVRELEEDKAHAVLFGHNPGMHEFAERILARAHVPRMPTCTAVIMALPHAYWGLVDW
                     NEAQLIGYVTPKALERRFPGLYAGISKPDMGLESAG"
     gene            21813..22808
                     /locus_tag="PLP58_05580"
     CDS             21813..22808
                     /locus_tag="PLP58_05580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ08019.1"
                     /translation="MIICLETSILGGHLSNEVPGLITGSLHLAGSRQPVQVELAGNFC
                     RDIAGCRVDLHNPLPDADLDDVAWVSPLQSGFSGVMTASYRVVKMPRRRSAGGKKLEA
                     PAGLKNLLFMEWFNKQGQRLLIQSWQIQVRVGAPCWKMSAEEESAMLRQARTRRKHFL
                     LNQREDGGSQDALLAPGLADPFEPHDLRGNPFDTLPENLPTREKESKSGGIDPAARAA
                     TLHEELRRFGNLLDPVVTPSVRPAMIQLLSTVADLAVHLMHALRHFKPGHPVHGRHLV
                     VDLEQSLPLFGAALNSVDKLASTPSMEAQRQWLARVQAGLLGIELRMRELLSLIR"
     gene            22942..24360
                     /locus_tag="PLP58_05585"
     CDS             22942..24360
                     /locus_tag="PLP58_05585"
                     /inference="COORDINATES: protein motif:HMM:TIGR01845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux transporter outer membrane subunit"
                     /protein_id="HRJ08020.1"
                     /translation="MIHLRPRHVSCLLCSMALASCALLPPLGSRRMAEDVSSLKGKAP
                     EKWSVLPSLPERESTGWLADFNSPKLQSLVSRAIQANPDLKAAAARVRQARAQTMEAG
                     AALFPAVSGSFNAVRSQTPADQRFSGLAFINNRFRLPLNVSWEADFWGRVADERRAAK
                     AGAQAAEESYHAARLSLAASTVNAAITLAEARSQANLAAQDVRTRQVQLAILEKQLDR
                     GLDPERAALDLSLSRADLARAESAFQQSRASADQAARALETLLGGYPAGTEPGLDTLP
                     ALKRNVPAGLPSEMLLRRPDIRAAERRLAAALGTESAAKKAFLPSFRMTGDYGRTTQF
                     LDTLLTPETAFWSIAGSTTQSFFQGGRVLAAARAARARYDELLHAYEAGALTAFREVE
                     TALAAEQFLNDQAAALSRAASEAETAERLALGQYERGLTPALTLLDARQRAFDARSAL
                     LAIQARSLRNRADLHLALGGEF"
     gene            24437..25840
                     /locus_tag="PLP58_05590"
     CDS             24437..25840
                     /locus_tag="PLP58_05590"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase"
                     /protein_id="HRJ08021.1"
                     /translation="MRQFLALLLLCGSITQAQPPNLLIILADDCTYNDLPVYGGQNAR
                     TPNLDRLAADGLTFNKAYVTAAMCQPCRAELYTGQHPMRNGCAWNHSASRPGTLSLPQ
                     HLAPAGYRTGIAGKVHVQPAESYPFENVTGFDPNCVRSPTEAHDLAGVRDFITRDKKQ
                     PFCLVVALTEPHVPWVMGDASEYPPKKLKLPPNIADTPKTRQDFAAYLAEITYMDGQV
                     GEILATLEQSGAAQDTLVLFSSEQGSQFPGNKWTCWDTGLHTALIARWPGKIAPGTRT
                     DALVQYADVAPTLLDLAGLKTNHPFDGQSFAAVLRGEKNTHRDFAYGAHNNIPEGNAY
                     PVRTVSDGEWRYIRNLAPDEIYIEKHLMGQLGGSAAHNAYWPTWMATAFDHPRTYQLV
                     KRYLRRPAEELYHTASDPGEMKNLAGSAEHAAIQAKLSAELDRWLAEQGDPGLPQDTH
                     ESHQAAKRGEHRFFPKP"
     gene            25905..27074
                     /locus_tag="PLP58_05595"
     CDS             25905..27074
                     /locus_tag="PLP58_05595"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HRJ08022.1"
                     /translation="MKICLALLLLVSPALALDPAKLAAIHPAMEEAVKAKQAAGIVTL
                     VMEKGKIVHHDAAGMADIAKGRAMEKDALFWIASMTKSVNGAAVMTLVDEGKLALDEP
                     ASTWLPEQANVTVEGGAKPEKPITLRMLLSHTAGIAFPPRKPTDGAISLKSYVATLIK
                     TPLAFQPGSSYEYGFGPTVAGRIIELACSMKYEDYLRTRLFEPLGMKDTTFHPNDAQR
                     ARIARTYKMDEDTRELVPGYNPFVTSDASVQHMPEPAGGLFSTAQDMGRFYAMIANNG
                     ELDGKRILSAKSVADMTKPVTAGEKQLNYACGWQCNIETQRVCAAMPVGSFGHGGAFA
                     TNGFVDPKSGIVTVFMVQNVLVPDSGKPRDVFQKLVMDAAGITVKPPLMAKKKQK"
     gene            27265..27855
                     /locus_tag="PLP58_05600"
     CDS             27265..27855
                     /locus_tag="PLP58_05600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009962803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HRJ08023.1"
                     /translation="MLAFLRCELRDADAARDMSHAFFAEMLGSGSIGGADRTRGRFRS
                     YLLGAVKHFVARQRESAARMKRGGGLEALSLDHEEAVEVVDTRQGTPDAEFDRQWAVT
                     VLARGLEALHLECLAENRETFFASVKPLLMGDGAYGDQAALAAICGMNLDAFRMAVHR
                     LKKRLRQCVKAEVAGTLDDPAMVQEEMQSLFAALGG"
     gene            28113..>31378
                     /locus_tag="PLP58_05605"
     CDS             28113..>31378
                     /locus_tag="PLP58_05605"
                     /inference="COORDINATES: protein motif:HMM:NF019334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HRJ08024.1"
                     /translation="MGVVYKARQKSLNRLVALKLLAPERADDPQFAARFEKEAQALAA
                     LNHPHIVAVHDFGQAGGFYYLLMEFVDGVNLRQLLQTKKLTPKEALSIVPPVCDALQC
                     AHDHGIVHRDIKPENLLIDKTGVVKIADFGIAKIYSSPLAPREEQSTPSHDAATMPFG
                     TPDYAAPEQANGAADHRADIYSLGVVLYEMLAGERPKENITPPSKRVQVDIRIDEIVL
                     RALEKAPELRFATAAEFRTQVEAAIHGEPAHTPNVECIVCRTCGHARSLDVTRGIQWS
                     ASQKERRMFMWCPQCGCPRVGERKMGVPSNAPSMPQIVESSSKITFAVMITLFYAGAA
                     LVSLLSMMRVGVLEALIAGAIMVVVLWKLGLLPKVSHAGCRRGLSWTAFALALPMLAM
                     GMFFVSQIPAETGGWNPGPAELVFVPLTWLGMLALPWAGAVLWHSTSRPVTHRTAARR
                     PGCLTIFLLVALAVAILAGVMITMWRLSGAASPRLEASCVIREVRDNVIILDTDVIAS
                     EGTAQVGFSFEGPALSADQLEQARRKVADRKIGVIGPHPDPHPRMVQGLLANHDRMSI
                     GLAFPTAEAAESARAAMKSPAEVQIQPEKHVQAHKILFSITDNAGQRIIGSMFLGRQS
                     LGHGAMIDPRGMISFTTDAVNVPPVRPGSSSDELKKLQTELGRLLTSRGEQHPEVLEL
                     RARIRSLQTPAAVGDIVGIGVALHVKNGRFVVAQIFPGSPAESLLKPGDEILRVGESD
                     DTLKDISAMSIEQVVDMIRGNAGTQVLIVVRSEGEDERKVTLVRESLGAAPVTTTLGG
                     DQKPASTMTQEPNTWIVEGRVVNDAGKPVAEANIHVSCGVGSLHTTGEGKTGADGRYR
                     VTFGPGFLVSIKDRAGSLQAALVHARKDGYTEKNLCESGNLQMAWELTKQQLAGGWQP
                     GPSRTFLPGKPLTVDFVLVPAAQIQGTLIDKNGKPVANQEIVITGDRIGPGGSIYGAT
                     RTDSQGRFKFKDVSTQHAWSFSIDDRSGHPERTPPDRFAKPETHRITLEMDGSTLKRL
                     NPPPTTTLQGTMNDNDLKQLSWHEFDQTKGKYWRELADVRRFKEAAELIERMLSLHPD
                     L"
BASE COUNT         6026 a         9947 c         9561 g         5844 t
ORIGIN      
        1 gggttttccg gcgatgagca ccagcttgcg tttgccgtcc ggtgtgattg cggaggcgga
       61 aacgcacaat gcgagcagca ggagaattcg tgtcatgcgg ccagcatgac ggcgcgcacc
      121 gcgcggctca agcgcattct acgagtctct gtcgttcgcc tgaaatccaa agcaagactc
      181 caaactgcgg agtgaaatcc ctcctccccc cgtatgaagt cccatgcaca cacgaacgat
      241 cctgatggca ggcgggctga tcattctcat ggcagtcccc tgccctgcgg ctgagtggga
      301 aaagctgccg cccctgcccg agccaaacgg cggcttcgcc tgcgcggcgc tcggtgaagt
      361 gatcgtcgtc gccggcggca cgaactggga gggcggcgtg aaaaactggc tcgacgccat
      421 ccacattttt tctcccaaga cacagtcatg gcggcaggcg ggcaggcttc cctcgcccca
      481 ggcttatgcg gtgtcggggg tcgtggagga cgaaaatgga tggccgcaac tcgtcatcaa
      541 aggcggcaca atgggggagc gcacagtgcc cgaatggcgg tccttgaatc atggcggcgg
      601 acaaaccgtg atggatattg cgccgcaccc tcgcgtcctt gccgcgggcg ggattgtgaa
      661 tggatggctg gtccaggttg gcggcacgaa tgacccggcc aatctcgctg gactggtgcg
      721 tgcagcattg ctgctcaaac ccggcgacgg cttgattgac atcttgcccg actaccccgg
      781 cgcgccattt ggcacggccg cctgcactgt ggcggcggca gagcttttca tttttggcgg
      841 agccaattgg gatgcatccg ccaacaccgt gcgcaacgcg gtcgaggccc acgccctgca
      901 tgtggcaaag cgcgcctggc gtcccttgaa acacctgccc tgccccgtgc gcggactggc
      961 agcggtggat ctgggaaatg gcagccttta tctggccggc ggctacaaaa gtgatacgga
     1021 gggattcacc gccgaggcat ggttttacga catcgccaaa gacgaataca agtccgccaa
     1081 acccctgccg tattctggca tggtgaactt ggtggtgtgc aaagggcatc tctactgcct
     1141 gggcggtgag gacaggcaaa agtcacgaac taatgcctgt tacagaatag gtctgcctga
     1201 gttggaagtg aagtagccca aacagtcggc ccaatgcttt aacacgcctt attatcaaaa
     1261 ttattaaagt ctctattatc atatttaacc atcatatttg ataataattt caaaattcga
     1321 atttgactga cttgatcact ctggaaattc aatcgagcat tctcaatgct cgcaaaactg
     1381 aattttatag ttaatcaagt tctcgcaaat gcgaaagctc tggtggtttt attggctttg
     1441 attgtctgcg ccggaccttc ccgcgccaat ctgggcacct tttatttcac gccattcgaa
     1501 ttcgcctatg ccggcgggct ttcctcaccc gcgctggaag gcagcttgtt cgccctggaa
     1561 gtacatgagg tcttcggcaa ccccaactcg gtctatctca gcctgctcaa cctcagtgat
     1621 ctctcccacc cgaacatctc cgccacctcg ccgacgatca cgggcatcta cttcgatgac
     1681 aacgccagcc tgctcaattt cggctcggcc agcattttcg accagtcgcc catgaccggc
     1741 ttcaacctcg gcggcggcgg tccgccgaat gtgcccgggg gggtgggcat cagtttcttt
     1801 tcagattacc gcgtttcagc cacgccgccg tctcccagca tggggcttga tcccggccag
     1861 ttcctcactg tccagtttgg gctgctcgga ggcgccacgg cgtcggatgt cttccaggcg
     1921 ctggcacatg aggaggcgtc ctccggcctg agactggcgc tgcatgtgca ggaaatcggc
     1981 gtcggctcca gcaccgaggc atccggctcc tttgtgaccg tggtgcccga gcctggcggt
     2041 ttcctcctgc tcgccgtggc gggcatggtg ctgcaactgc gccgccgccg ccgttgacag
     2101 tgggcggaaa tcccggccac taatctaccg ggccattttc ctcgaaatcc cttgtcgcaa
     2161 aatcagcggg atgcgccagc ttggccgccc ctttgccatg gccaacgaac gcaagaccct
     2221 cgaagaacgc gccctgatgt cagacatcga gcgcctgcgc cactcctgcg cccatgtgat
     2281 ggccaccgcc atcctgcgcc tctggccgga cgcccagttc gcctacggcc cgcccgtcga
     2341 aaccggcttc tattacgact tccagatggc ccaccgcatc acgcctgacg acttcgaaaa
     2401 gatcgaggcg gagatgaaga agatcgccaa ggagaaccag cgcttcgagc gccgggtcat
     2461 cagccgcgag gaagccatcg ccctcgccaa aagcggccgc ctcggtggcc tcatcgagcg
     2521 ccccggcaac gagagccgct tcaagctcga cctcatcgaa aaaattcccg agggcgagga
     2581 gatcagttgc taccaaaacg gcgacttcct cgacctctgc gccggccccc acgtcggcta
     2641 caccagcaag tgcaaaaacg tcaagctcac cgccgtcagc gcctcctttt acatggggga
     2701 cgaaagcaaa ggccagctcc aacgcctcta cggcaccgcc tttgaatcca aggaagagat
     2761 cgaagcctat ttcgtccgcc ttgaggaagc caaaaagcgc gaccaccgcc gcctcggcaa
     2821 agaattgaag ctcttccaca tagacgagga cgtcggccag ggcctcatcc tctggacgcc
     2881 caacggcgcc gtcatccgcc aggagctgca aaacttcatc agcgaggaat tgcgcaagca
     2941 aggctaccat caggtcttca ccccgcacat cggcaaactg gccctctaca agaccagcgg
     3001 ccacttcccc tattacaaag agtcgcagtt ccccgccatc gtcgagcagg atcagctcga
     3061 gcagatcgcc catgaaggct gcacctgcgc ggagatgacc gcgcgcctcg acgccgtctc
     3121 cgcccagttc gcctcccaga tcaacgagcg caccggcacc gaagtcatcg gccaggaccg
     3181 cgtgctcgac gccgagaagc tgctcgacgg cttcctgctc aagccgatga actgcccgca
     3241 tcacatcaaa atcttcgcca gccagccccg cagctaccgt gacctgcccg tgcgcctcgc
     3301 cgagtttggc accgtctatc gctgggagca gagcggtgaa ctcaacggca tgacccgcgt
     3361 gcgcggcttc acccaggacg acgcccacct cttctgcacc gaggagcaag tcgccgccga
     3421 agtgctcggc tgcctctccc tcgtcaagac cgtgctcacc accctcggca tggaggacta
     3481 ccgcgtgcgc gtcggcctgc gcgacccgga cagcgccaaa tacgccggcc gccccgagca
     3541 gtgggaccgc gccgagaccg cctgccgaga ggccgcccaa tccctcggcg tgcccttcac
     3601 cgaggagccc ggagaggccg ccttttacgg cccgaagatt gacttcgtca tcaaagacgt
     3661 catcggccgc gaatggcagc tcggcaccgt ccaggtggac taccagctcc ccatccgctt
     3721 cgatctcagc tacaacggcg ccgacaacaa accccactgc cccgtcatga tccaccgcgc
     3781 ccccttcggc agcatggagc gcttctgcgg cgtgctcatc gagcacttcg ccggccactt
     3841 ccccgcctgg ctcgcccccg agcaggtgcg cgtgctcacc atcagcgaaa aagtggacgc
     3901 cttcgccagc gaggtcttcg cccagctcaa agccgccggc ctgcgcgcca cgctgaacaa
     3961 cgaggccgac aaaatcggcg ccaagatccg caacgcccag ctcgacaaag tcccctacat
     4021 gctcgtcctc ggcgagaagg aagccgccgc cgccagcgtc gccgtccgcc acagcaaaaa
     4081 aggcgacctc ggcgtcaaac ccgtcgccga cttcctcacc gaaatcaccg ccgaggtgcg
     4141 ggagcgcagg ctgtgagctg attcccgtga ctctcaagtg agcgcaagac gactttcccc
     4201 tctcatccag ccactccacc agcagccttg gactcgctgg cagtcccctg gccagatcgt
     4261 catgagtggg gcatgaattt tccgccggct cttatttcat gagcgcatct ttttggcgcg
     4321 cttgtttcgc ctggggaaaa ccgcccttca gtccgccgtt tacccgcctt ctcttccagg
     4381 cgcctccgca gccacgggct tgcgcacttt ttgggcgatc ttgtgcagaa tgccgttcac
     4441 gaaggaaccg gactcaccac caccgaggct ttttgcgatc tcgatggcct cgttgatgac
     4501 caccgggccg ggcacatccg gcaggtgcag caactcatgc acggcgagcc ggagaatgtt
     4561 ccggtccacg gcggcgaggc gctcgatccg gaagttgtcc agcacggggc ggatgtgcgc
     4621 gtctatttca tcgaggtggc tgagcactcc ctgcgcgagg gattcggcat ggctgcgcac
     4681 ggatgccttg gcggagtgga tgctccaaaa ctccccggca ttggcgggtt caccctcgcc
     4741 ctggagttcg cgggcgaaga ggtattgcac ggcggcggcg cggccttcac gtcgttttcc
     4801 catggtactc aggcgtcctc caatcccgtt tcgccggcaa agctggcgcg cattttgtta
     4861 aagaggctga tcatctgcac ggccacccgg ccggcttcgg tgccgcggtt gatgctttcg
     4921 cccaggcagc gctcgcgggc ctgctcctcg ctggagacga gcagcacctc gtgaacgacg
     4981 ggggtgcaat gcgccacggc catgcgctgg agcgcgtctg tgacggatgc tcccacaagg
     5041 tcgccatgtt cggtggcgcc gcggatgatg acaccgaagg cgatcaagac atccggcagg
     5101 ctgtggcgca gcaccatctc agcgcaaacg gggatctcaa aagcgcccgg cacgcgatac
     5161 acggtaatgc tggcgttcgg ggcgatctcg ttgatctcct cgcagccggc gtcgagcagg
     5221 ccctggacgt aggtgttgtt gtaaaggctc gccacgatgg cgatggagac cggctcgtgg
     5281 atcgtgcgcg ggcggctggg tccgtgttgt gacatggtgg tgagatgcgg ggctgggtga
     5341 gtgggcaggg aaaattcgaa gctcgaattt cgagcttcaa agcctgtgcc cgagtttttt
     5401 cttcttggtt tcgaggtatt tcttgttgtg cggattcgga tcggaaatga cgggcacctg
     5461 ctccacgatc tccagcttgt ggccttcgag gcccaccacc ttgcgcgggt tgttggtcat
     5521 gaggcggatt ttccgaacgc ccaggtcgga gatgatttgc gcgccgatgc cgtagtctcg
     5581 caggtccatg gggaagccta gcttcagatt cgcctccacg gtgtcgaggc cctgctcctg
     5641 gagtttgtag gcgaggatct tgccgtgcaa gccgatgccg cgcccctcgt gcccgcgcat
     5701 atagacgatg acacccgcgc ccgcctccgc caccatgcgc atggcggagt ggagctggct
     5761 gccgcagtca cagcggcgtg aggtgaagat gtcgcccgtc aggcactcgc tatgcacgcg
     5821 caccagggcg ggcttggcgg ggtctatttt gcccatcacc agcgccacat gatgcactcc
     5881 atccagggcg ctcttgtaca aatgcaggcg gaaggtgccg aagtccgtgg gcatgtccac
     5941 atcctcgatc ttttccacca gcttctcact acgccggcgg tggccgatga ggtcggcgat
     6001 gctgcccatc ttcagcttgt gcttgcgggc gaatttcttc aggtccggca gccgcgccat
     6061 ggtgccgtca gggttcatca cctcgatgag cacgccagcc tcccgcaatc cggccaggcg
     6121 gcacaggtcg gtggccgcct cggtgtggcc ggcgcgccgc agcacgcccc cgggcttggc
     6181 cacaagcgga ttgatgtggc ccggttgtac gaagtcctgc gtgctgctct ccggggccgc
     6241 cagcaggcgg atggtgcgcg cgcggtcggc ggcgctgatg ccggtgctga tgcccttggc
     6301 tgcatccacg gtgacggtga agtccgtgcg aaatgcctca cgattctccg gcaccatgct
     6361 ctccagcccc aggcggtggg cgatctccag cgacaccggc acgcacaaca tgccgccgcc
     6421 ctcgcgcacc atgaatgtca ccatctccgg tgtgatcttt tccgccgcgc agatcaggtc
     6481 gccctcgttt tcgcggtcct cgtcatcagt gatgatgacc atgcgcccgg cgcggatgtc
     6541 cgcgatgacg gactccatgg gatcgcagac atgttttttt tttgctggca tggggaggtt
     6601 ggaaaagata cttgccgccc ggttccgtcc gggctgcgag ggtgcgggag gtaagtcagc
     6661 ctccggcgca tttccagcct ggatttttat cttccgcaaa aaggccggct accggggccc
     6721 atgctcaacg cccaggcacc agtctggcga gatcctccgg cagcggagac tggaaacaca
     6781 tctcccgccc tgtggccgga tgctggaaac gcagggcagt ggcatgcagg gcgagccggc
     6841 gggtgctcga cgcgggcttg ccgccataag tcacatcccc gacaatcggg tgcccggcgg
     6901 ctgcgagttg gacgcggatc tgatgccggc gtccggtttc caaagtgatg tccagcaggc
     6961 tgcggccata tccctcgcgc acgacccggt agtgggtgat ggcttcgcgc gtggacggtc
     7021 cggggcgggc ggcggcataa acacggtggg gctggctttc gtccaggtgc ccgcgcaatg
     7081 tgccttgttt ttgctccaac gcgccttcga caacggcctg atactgcttt tcagccagat
     7141 cccagctttc ctgaagggcc agcttcgcct cctcggtcct ggccagaacg atgaggccgg
     7201 aggtgtcgcg gtcaagacgg tggacaatcc acagccgcgc cctggcatca ccagtgcgct
     7261 cgcgcaaatg atcggtgaga aagcggaagg cggtgcgctg gcgctccgtg tccgtggcca
     7321 cagtgagcaa accggcgggc tttagaatga cgaggatgct ctcatcctcg tgcagcaaat
     7381 gcagtcctgc cggaagcggc gcgacctgcg ggggggcttt ccgcggacgg atggtgatga
     7441 catcgccggg ttgcagggga tggtcgtgac gggtgacggg acggtcattg acatgcacgg
     7501 cgccgaaacg cagccactgc ctcatcttgg tgcgtttcac ctccggccag aggcaaaaca
     7561 aatgatctag gagtgcggcg gaggcagtga cagtgcttga aagcatgagt caatgaagac
     7621 cgggaaggac aaaagtgaca ttcgaaattt cacaggccgc cttcgaaggt ctgaaggaaa
     7681 gtgactggtg actgaaaagc aggcggagcg gcttgttcca gacatcaaga catggcaggt
     7741 tttgaacatg catgctcaag taattgccta ggaaatgaaa aagagattgc ctttcagaca
     7801 agtacatgcg ttccattcaa catgacccat caaacggggt ttccttttaa ggcgtggaaa
     7861 ttcagactcc accctgcggg ataccccgga gtggtttcaa attgtcaaac acccgcagac
     7921 gcgggtcatg aaacaacttg gactgcttcg atgaaaagca accgacccac ccacaacagc
     7981 acagaccatg aaacataaaa tctaccttct ggatgaccat ccaatcgtca ttgagggact
     8041 caaaaaaatc ttcgagggcc aggaagacct tcaattcgtt ggctactccg aggacgccaa
     8101 caacgcgctc gtcgaaatcg agaagctcaa gccgaacctc gtcatattgg acctgaccct
     8161 cagggaccgc agcggcgtgg atctgatcaa aaaactcaag cagtcccatc cgagcatcag
     8221 cgttctcgtc tattcgatgc atgatgaaaa tgtttatgcc gagcgctgcc ttcgcgccgg
     8281 cgccatgggc ttcatcatga agggggaacc cgccgaacgt gtcatccagg ccattcgtca
     8341 ggtgctgggt ggcggcttct ttttcagcgc cgctcttctc agcagcatcg tctcaggcgg
     8401 cggcccccgt tccggctccc gcggcgagcc cggccgcatg ctcagcgacc gccagctcga
     8461 agtcttcgag atggtcggcc gcggcttcga ctcccacgag atcggcgaga agctccagct
     8521 cagcgccaag acggtggacg cccacaaggc caacatccgc cagaaactgg gtctcgactc
     8581 cgcgcgcgaa cttctcatgg aagccgtgcg ctgggtggaa aaataagccg cccgtccaag
     8641 tgtgctcaac cgatgccagg aggttcgcct cctggcattt tttttcgcgc gctcaaagaa
     8701 ccgcggcgcg cacaacatcc ggactgatgc cgcgcagttg gaagacgcgc agcagcgtgt
     8761 cctcgatcag gttgttcggg caggaggcgc ccgctgtgat gccgatgacc accgggtccg
     8821 gagaggccag cttgccggag tagctggttt tctccgactt cgaatgcaga tcgaaatgca
     8881 caatctgctc cagcgactcc agacactcag ccgtgcggat gaaaaaggtc gggagttgct
     8941 gctcgccaat ctcgacaagg tgcgtcgtgt tcgagctgtt gtagccgcca acgaccagca
     9001 gcacgtccaa cggatgacgc agcaaatgaa aaagagaatc ctgccgctct tgcgtcgctc
     9061 cgcagatggt gtcgaagacc aggaaatttt tcgccccctc ctccggaccg tcgcgatcct
     9121 ggatggcctg atgaagacgg cgctgaatct cctccgtctc ggatttcaac attgtggtct
     9181 gattcgccac gccgatgcgg cgcaggtgtg tgtccggatc aaacccttcg gaaagcgcgc
     9241 tggcaccttt gaatcggtcg agaaaagcgg ccttgtcacc gccattgcgg atgtgatcgc
     9301 agacgaaatc cacatccgcc agggtaagca ccacgagata atggcccctg ccatcgtcac
     9361 ccagagcgcg tgaggaggtg gcgcgcgtct cctcgtgctc cgccttgccg tggatgatcg
     9421 aggtgaagcc ggtcctggcg taatttcgca cgcgtttcca cacgctcatc acgtccccgc
     9481 aggtggtgtc caccaccatg cagccgcgct cggcgatctt ttgcatcaac ctcgtctccg
     9541 cgccaaatgc cggaacgatg accacgtcct cgctttgaaa atccgcgatg gcctgctcgt
     9601 gccggccgac cgggatgctc acaattccca tctcggtgag ctggcggttc acctcggggt
     9661 tgtggatgat ctctccaagc aggaagacac gcctttcggc aaagaccttc cgcgccgcgt
     9721 aggccaggtc tatcgcccgc tccactccgt agcaaaaacc aaactcgcgc gccagccgca
     9781 ccgtggtgcc gtcaatcgtc agcacacccc cggaggcgcg cacggcctcg accagcgggc
     9841 tgcggtagtg gctctcaacc tctgcctgca cacgctccat cacctccggc gtgcggacat
     9901 tgacacgacg ggaaccggcg ggggctggcg ctgcggacat cgggatcagt aattgtaaac
     9961 cttgccctgc gcggcgtcag tgaagacctg gctgctcagc atgtaggagt caggcgggtc
    10021 gggcagggtg gagtcatcca gcacgggcag gcttttgcca tgcgtggcgt cctcgggatg
    10081 actggcggcc acatgcagag gcgtgccgac ggggaccgcg tcaaagagct tcttcgccgt
    10141 cttgatgggc aggcgcacgc agccgtgggt gcaggggtag ggcttcaccc agccccagtg
    10201 cattccatag gcgggcgcgt aaaacgacat ccagtaagtc atcgggtaac cggcgcctgg
    10261 cgagctgaca cggcggcgct gggcggtctt gccggtgatg cggtggtgtc cggcgggagt
    10321 gggcgagctg gccgtcccca ccgcgcaggg gctggcgagc agcacgcggt cgccttccac
    10381 cagataaatg cgctgggctc cggtgctgat cttgagcttg agcgcggagg gatttttcac
    10441 cgcctccatg ggcggatcga agcgtgtgga gtaattgccg cccctgggct ttttggtgac
    10501 gcaactggca agcccggcgg cgagcactgc aaggacgaga aaacgcagga catggggaac
    10561 aggtgaggat ttcatgcgaa ggccacgctt agcacgcccg gcgcgggcat ccagcagttt
    10621 tcagcgacgg aaaatccggg cgttttttgc tgacaccgcg cgttccagtg aggtaaattc
    10681 gcctccaccg atgaaccacc aactgctgac cgccctgctt ttttccctgc cgctggccgc
    10741 ggccgcccag actcccgatg cggagccaaa gacggagtgg aagcttttca ccggcaaatc
    10801 cctcgacgac tgggagaccg tggacatcgg cggcagcggc gaggtcactc tcgaaggcgg
    10861 gcagatgatc atcgcccagg gcgacagtct cagcggcgtg gtttacaaaa aagtcgccga
    10921 actgcccctg accaactacg agatcaccct ccaggccaag cggctgcagg gcgtggactt
    10981 cttttgcggc ctcaccttcc ccgtcggcaa gctggaccgc tgcgccactc tcattctcgg
    11041 cggctggggt ggcagcgtca ccggcatcag cagcattgac gacctggatg ccgccaacaa
    11101 ctccacaggc agctaccagc gcttcgacga cgacaaatgg tacagcgtga ggctgcgcgt
    11161 gacgccggaa aatctcagcg cctgggttga tgagaagcag gtcgtggacg ccgacatcaa
    11221 tggccggaag gtcaatctgc gtcccggtcc catcgaggcc tacgccccgc tctccatcac
    11281 cacattcacc accatggctg ccatccgcgg tgtgaccctc aaaaagctgt gacctggccg
    11341 cccttcacgg aaacgcgccc agcagcacgc gcccggattt cctgaccgct ccatccggca
    11401 gtagaaaata gccgtgtccc tgtacggaac caccggtccg caccagccgt gcctgccaca
    11461 cagccttgcg cggcggaacc gtggcgagaa gaaaatcacc cgctatctgc ccggtcttgg
    11521 tgtccagctt ggatattttc acccggttgg ggttgatgtc caaagccgtc ggcacacgcg
    11581 ccttgagagt gacgccggca gcgcttggat tgaaaagggt gaccagaaga tcgaactcca
    11641 tgccaacgtc atccagcccg cccgcgctga agtccagcag cgcgttgtca tcgccaatgt
    11701 cgaagcccat gagcattccg cctttgtcaa tcatcgggta gccgccgccc tccgcgtcca
    11761 gcatcagcgg gccgaatcct tcttgataaa gcgtgtcctt ggacttcgcg tcgcccccgg
    11821 gtttgaacca cgaaagcgat cccatgacat cattgtccac cggctcgaat ccaggcgtga
    11881 cttcaagcac tcctgccagc gatccctttt ttgcgtacaa ggaatgatac accagcacct
    11941 ggccgctgcg accgatgaag gtcttcgtcg cgaatgccat gccatcgccg agattcccgc
    12001 ccaccgtgag tccgccattc gcctcggcca cggtgaaggc gccaaagcca tccccctgcg
    12061 gaaaagccgc ggccgggtcg gactggatga ggcggaagga atgcgggcct ttgaagtcgg
    12121 ccgcattgtc gtctttcgtc caggcattgc gccaggcgtt cactggtgtg tcatttccgc
    12181 cggaggcgag atcacccgcc accaagttgt catccgcgtc gaaaaacaat gacagcgttt
    12241 gtggatccgc acctttgggc tggagcatga ccgtcagagc cggatgatcc tcatcgtcca
    12301 catccgcgtc gagctgtcct ttgaaaggca gcgcggtggt gcctgtgatg accttgccgg
    12361 agaccgagcc ggtgctagcc acagtcatct catagcgcga gccgagattg ccgttgagca
    12421 gcgggtgcgg ctcaatgagg ccgtggaaga gtccggcgat gccgtcgggg agaaaaccca
    12481 cattgatgtc gaactcgagg gtttcatccg ccgcgccagc ggcgtttttg gccttgatca
    12541 ccaggctgta ggcgccgccc ttcagtggct tgccgctgat gactccggta tttccattgg
    12601 cagtcagtcc gggcggcagg ttcttgatcg taaatgtcac cggcagattc acgaccggga
    12661 tttgcagcac atagtccccg ctcactgtgg ccgaatctat ctgcgccggc atggcgagag
    12721 cgggcttcaa gacacccgcc tgggtcaccg tgtgcgcctg accgccgatg agcaccgtgg
    12781 cggtgcgctg cttcaaagaa gtattggcca gcacggtgag agtgacggtg ccatcaccca
    12841 tgcctgccgg tggcccggag atgatccaag gcggcgtctg cacggaccag agtccgtccg
    12901 aggtgacttc gatgtcatgg gatccgccaa cggaggcgag atcgcttttt tcactcaaat
    12961 ccagcatgag cgtcccaggc atcaacgtca ccgccgcgaa tgcggagccg ccgaaggcat
    13021 ctttcaccgt gtaggaaaag agcgtcggcg tggaaaaatt cgccgccgtg gtcagcacga
    13081 gggtgttgcc cgtctttttc aggctggcac ccgcccccgg ctttgtgaag gaggccagca
    13141 acagcgcgtc gccatccgga tcgaagtcat tatcgaagac atcgaggacg accgtgcttc
    13201 ccggcagcac ggtgaaggtg tcattgactg cggccggggc gcggttcgcc gtggtgaagc
    13261 tcatggtggc tccattcgcg gccacttgca cgccggcggc gcgcgcgcga taatgatatt
    13321 tcgtgtgcgg attcaacccg ctcaacaccg cgctcacatt ttccactcca gagccatcca
    13381 ccgtcgcagg gtcagccgcc acagtcatgc ccaatgccgt ggtggtgccg taatcgaaga
    13441 aaacttccct ggcgaagccc ttcgcatcca ccgtgccgcg cagggtggct ccctcaaaag
    13501 tcgcatccgt tgcggccagt gttttcacca ccggcggcac tggcttgcca aggatgccga
    13561 aggtgtgaaa gttgtccatg aacacctgat gagcgtctgt aacctgcgtt tcctccgagt
    13621 aggcgccgag atggattttg aagctgccgg acgctgtcat tccccagccg ccggtgacgg
    13681 gattaaaggt cctgaagact gtccaggagt agccattgag cggaccgtcg gggtcataag
    13741 ccgccgacaa ggttttgctg ccggcatccc atgagatgcg cacggcggca tgagtggagg
    13801 gggcgtcctc aaaggtttcc ggttcatcct catcattgaa gtccacctca ttgaaaaaat
    13861 gaaaccgctg cgggctgtca aaatcccggt agttttccag ccaggcggaa aatctgtccc
    13921 ttccatccgc gctgtggctg acaaacacac cggccccgac cgccgtaaaa ctgccagaca
    13981 aaggtatcca gggcacgcgc acatccgctt gcacgctcca gtcgctggca aaaggcgcgt
    14041 tcaccagcca gggccagacc atgaaatcct catcggaaga tcctgcgctg gtgaaacgca
    14101 gcacgccgcc agtctgggtg agcagccccg agccttcgct gatgttgccg ccccatttgc
    14161 tggtgttttt gctggcgccc tcaaaatcat cctttcctgt gggactcccc gcgacaacgg
    14221 actggacggc gctgatcgcc tcgaaattgt ccgcgtaagc atcgccggaa ttcaccgtca
    14281 tgccctgatg ctcgccaaag acagccagtg tgaaacgcga tgacgcgtcc atcatccagt
    14341 ccgtggcggg cgcgtccacg tccaccgttt tgagcagcgt ccaggcatgg ccgccttcgg
    14401 gaccgttgaa gtcataataa aaggacagca tctttgatgc cgcatcgaag ctgattcgca
    14461 gtgagccgtc tgtcgagcta gtatccgcgt gggtctcgcc gacatcgcct tccgtggtgt
    14521 agcgaaagcg tctctgcggt atgctgcctc ccacatgttc gacttccagt tgcacctcgc
    14581 caacggagtc tcctccataa acagccagca ccatctgcag gcggtccccc tcctccgcca
    14641 ggctcacatg ccccacatgc acatccaccc gcgcctccca atcctgagtg tagctgccgg
    14701 tgttcagaat ccatggccgc tcagagtagc cgaaaggcga ggccatcccg tcccccgtgt
    14761 attcgagtct cccgttgacc tcgttcaatg cccccgtgtt gccggagtcg tcaggacccc
    14821 attttgtcgc gttcctgtcg ttgtcgttga aattgtcgcc accgaacaaa ttgaccagga
    14881 ccatttctga aacggaaaag ttgtccgcat gcatgttcat cactcccgtg acctccgttt
    14941 cctcggaata ccctccaatg agaatttgaa acacatcccc ttccgtcatg ttccagcccc
    15001 cgccggccgg gttgaaagac tgcaaagcgg tccatgcctg gccgttggct ggcccgtccg
    15061 cgtcatactc cgccgtcaaa gtgcgtgtga ccgcgttcca ggaaattcgc accgtggcca
    15121 gatgattgac ggcttcggca aaggtctctg tcgtgtcgtc atctatgtcc accgcgttga
    15181 aaaaatgaaa ctgctgagcc tctccgggat ttcgatagtt ttccaaggcc atggaaaagg
    15241 cgtccgtgtc atccagtcca ctgcggacga tgatgcccat gcctgcggcg gtatggccgt
    15301 cggcaagggc aatgtgaggc acatggacat cgacctgcac ggaccaactg ctgtcgaacg
    15361 gggcgtcggc cagccagggc cacgccatga agtcatcatc cgtcatcccg tcgctggtaa
    15421 agcgcagcac cccgcccgtc tgggcaagct ggccggaacc agacgacaca tcagcgcccc
    15481 atttggattc gtttttggcg gcgccggaga agtcctcgtc acccaggaat tgcgcgtgcg
    15541 tttgagccgc cgccagaagc agggcgcccc caagaaggat cgttttcata aaggtggatt
    15601 ttgttaatcc aaagtgggtt caaaaacgct ggatggcgaa tgatttgtgg gttgcgcgca
    15661 ctgaacggca aaaatcgccc cattcgtccg gagcatctca agcgcgccaa ctccgcctgc
    15721 atccgaccta gccaatcaga taaatcaccc gccgcagacc tgaccgctcc tccaccgtgc
    15781 ggcccacggg ccggccaagc acgcgctcga tcatctcgca ttcgatctcc tcgaccagcg
    15841 gatacttttt caccacctcc accagcggga aatgataatc cacgaggcgg agatcgccgc
    15901 cgggcgacat ctccacctcg caaaggcggc cctcctggct gcgcaggcgg gctatcacaa
    15961 tggctttgtt ttccagcagg ctctccttgt ccgcagcctc ctggtaacgc gcggcacgct
    16021 cctgatagaa tgcgtagagc agcttttccg cggcagtggg accgaacacc cgctccgcat
    16081 gtcccagcat ttcaatgagc ggatcaggag ccccaccatt gaaaagcggg tccagctttt
    16141 gcgtcagccg gtagatcttc tccgggcggc catgcggcac agggtggcgc caggtgtcga
    16201 ggcagccctt tttcaccaac gcatcgcagt gctgcttcac ccccatgtag ctcatcttga
    16261 gcgcgccctt cagttcgttc acagtcatgc cgggagactg cttcaagaga cggatgatct
    16321 ccagggcagg cgagggcacg agttctcgaa ggagattcag gatcatggcc ggtgatgccc
    16381 gtcgagacta gcggaggttg cgtctttgcc aagcccgcaa acaacgcgcc gccttcagtt
    16441 ttgcgcccga tacggaaaaa cttatccaag atgcatgggg tccacatccc atgtgacgat
    16501 gatgtccgcc ggcagggtgg tggctttcac cacggcgcgc agatgggcgc acaggggacg
    16561 gatggcgggg ctgctcaaga ggagctggaa gcggtgctgg ccgtgcgcgc gggacagcgg
    16621 cgcgggcgcg ggatggccga ggtaggcgcc ctcgggcaga ccttcggcta ggcgcttgcg
    16681 cagcgtttgc agggtgaact cggcctgcgt ttcatgcggg ccgcgcgcac tgagcaggac
    16741 gacgtgactg aagggcggat aggccagcgc tttacgctgc tccagctcct gatcggcgta
    16801 gccctcgtag tcgttgtggc ggctgaactg gatggccgga ctgtgcgggg cgtgcgtctg
    16861 cacgaagacc tcccccttaa cctcgccacg cccggcgcgc ccggccactt gcacgagcag
    16921 ttggaaggtg cgctcggcgg cgcgaaagtc gggcaggttc agggcggtgt cggcattgag
    16981 cacgccgacg agggtgacat tcgggaagtc gaggcctttg gcgatcattt gcgtgccgat
    17041 gaggatgtcg agcttctgag cgcggaagtc gcgcaagatg tcacgcagtt ggtttttccg
    17101 ctgcatggtg tcggtgtcca cgcgggcgct gcgggcctgg gggaagacct cgcgcacggc
    17161 ggcctcgacg cgctcggtgc cgaagccgcc gtatttgagg ccgggctcct tgcacgcggg
    17221 gcatttcacg ggcggcacgc gccgggcgcc gcagatgtgg cagacgaggc ggttgtcctt
    17281 tttatgcagc gtcatgggga tggcgcagtc ctggcattgg acgacctcgc cgcaggccag
    17341 gcaggagagg ctggtgttgt agccgcggcg gttgaggaaa agaatcgttt gttcgcgctt
    17401 ttcgagccgg tccgtgatgg cggcgcgcag gcgctgggaa aggatggcgg cattggcggc
    17461 agagacctcg gtgccctttc gccgctccag gcgcatgtcc acgatgcgga tgagcggcat
    17521 ggatttgcca tcggtgcggc gcgtcatgcg caggagctgg tatttgccgg cgcgggcgtt
    17581 ttgaaaggac tccaggcagg gcgtggcgga gccgagcagc acggcgcagc gctccaaatg
    17641 cccgcgcacc acggccacat cccgcgcgtg gtagcgcggc gcgtcctcct gcttgtagga
    17701 gggctcgtgc tcctcgtcca caatgatgat gccgagattc tccaaaggcg cgaagatggc
    17761 gctgcgcgcg ccgatgacga tctcggcgcg accttcatgg atcttgaacc actcatcgtg
    17821 gcgttcgcca tcgctgaggt ggctgtggag cacggccacg gcgtcttttt tgtcggagaa
    17881 gcgggctttg aagcgctcga tggtctgcgg ggttaggctg atctcgggca cgagcacgag
    17941 ggcggtgcgg ccttgcttca gcacttcggc gatggcctgc aaatacacct ccgttttgcc
    18001 gctgccggtg acgccgtgga gcagcagcgg gcgcggcgcg gtctcgcccg tgtggttcac
    18061 ggctttcagg acctcggcga gcgcggcggt ctgctcgtcg gtgagggtga gggcctggct
    18121 gggcagaaag tcttcagtct gaaaaggatc acgctccacg cgcaactcgc tgcgggtgat
    18181 ccagcccgcg gcgagcaggg gtttgataat ggcggtggct cgcggcagct cgcggcgcag
    18241 ctcgctgagg gtggactcgc cgccgctgga gcggagcttt tccaggatgc gcgcctgcat
    18301 gggcgcggtg cggtggagtt tgtcgaagat ctcgggaggc ggctctttag ccagcttcag
    18361 gtggctgtcg gtgagaaagg tctcgggctt ttcccggacg gcctggggca gcatggtgcg
    18421 caggacgcgg ttcactggca ccacatagta accggcgatc cagtgcgcca gcttcagcag
    18481 gcctgaggta aacatgggcc gtgcgccgat gagggcgaag atttccttca agcgcccgcc
    18541 gtggacggtc tgctccggca gctcgatgac cacggcgcgc acgcgctggt tttgcatcgg
    18601 cacggtgacg cgcgagccga ggcggatttg atccgcgagc ttttctggaa tgaggtaatc
    18661 cagctccagc gcggctgcgg tctcgacctg cacgcgggcg attttttgcc ctcccggctc
    18721 ggacacgcct tcctcggggc cgaaaaggcc gaggttgggt gtgtcagaag gcggaggcat
    18781 ggcggggtgt gtcagtcgga gagtgtgtgc ggcggtgaag ccgggcacat gacagcgcgc
    18841 gccatcaact cctcaactca ctctttatct ccgccatcct cgttccgcgc aagccggcgc
    18901 ttgaaatgta gctgctggac gcggcgcgcg tcagcgcgcg tggtgatgac atcgtagctc
    18961 tcgatgacca cagtctcgcc aatcctgggc aaatggccga ggagatgggt gacgtagccg
    19021 ccgatggtgg tgacctcgtc gctctcgata ttgatgccgg cctgctcggc cagttcgtaa
    19081 aggttgagcg tgccttcgac gacgaattca tcatcattga cgcggcggaa ctcgctggtc
    19141 tcgtggtcaa actcgtcctg aatgtcacca acgatctcct caatgacgtt gtcgagcgtg
    19201 acaattccaa ccgtgcctcc gtactcgtca accgccaggg cgatgtgaac gtgcttgtcg
    19261 aggaagaagc gcaggagctt gtctattggc atcatctccg gaaccatcgg caggtcgcgc
    19321 ttgacgccgc gcagatcggc gccggacttg ccgaccaagc cgaggagatc cttgatgtga
    19381 atgaggccga tgctttggtc gaggtggcct tcgacgagcg gataacgggt gtgcttgtgc
    19441 tggatggcgg cggcgagact ggcctcgaag ctgtcgcccg cgtcaaggca gacgacctgg
    19501 ttgcgcggcg tcatcacatc ccgcacgcag cggtcgttga gcgccagggt gttgagcagg
    19561 atgtccttct ccgtgtcggt gacctcgtcg gactcgctgc tggcggcgac gatgtggcgg
    19621 agttcctcct cagagtgggc cagctcgctt tcagaggcgg gttcgagacg gaagagcacc
    19681 ttcaagagcc agttggcggt cccattcagc acccagatgg cgggcttgaa gacgatgtaa
    19741 aaaaaatgca gcggcctgct gatggcgagc gtggtgggca gggctttgcg gatggcgagc
    19801 gacttcggca tcaattcgcc gaggatgacg tgcagatagg tgatgaaacc gaagccgatg
    19861 ccgatggaga cggaggagat gaccgtgtca ctcgtgatgc ccagcttcca catcaagggc
    19921 tggatgatgc gcgcgaggta aggctcgcca atcatgccga gcgcgatgct tgcgatggtg
    19981 atgccaagct gggtggcgga gagataaccg tcgaggtttt tgatctcggc gcgcgcggtc
    20041 acggcggcgc ggttcccctc gtcggcgagc gcgtcgagct gggtgccgcg cactttgacg
    20101 atggcaaact cggcggcgac gaagaaagcg ttcaacagca cgaaaaagag caccagggcc
    20161 gcggaccagg ccacctcgcc cgcagtcagg ttccactcat gcagcacctc gtgcgaaccg
    20221 cccagggcga gcacagggat tggatgatgg agcaggttca ttccttggtg ggcggctcgt
    20281 ggacggtgat ttcgccgggc taaagacgct cgtaggagcc gtcgcagcgt ttttcgagaa
    20341 tcgtaaaccc ggcctttttg gcgtccgtga cggacagcgg cctggtgatc accggtgtgt
    20401 tgaaagtgct gaccaatttt ttcaccggct gcctgcacaa tggacagtgc gcgagcgcag
    20461 gcctggacat ggggcgcctc aaatcaaaac ccctcctgca ggaggggcat cctttttccg
    20521 ggtcttcggc gatgtaggaa taggtgggca tgtcggaagc gctcacaccc tccgtggcgc
    20581 atccagggag gcatgtccct ggatgcgcgg ggcaaaacct taccaactgg atttggtgac
    20641 accgggaata agcccggcga gtgcgcactc gcggaaagtg aggcgggaca tctggaagcg
    20701 gcgcatgtag gcgcggcggc ggccggagac gcggcagcgg ttggtgagac gcgtggggct
    20761 ggcgtcgcgg ggaagcatgg acaggccgac atagtcgccc tgcgctttca gctccgcgcg
    20821 gagcttcgcg tatttattga cggtcttttg tttgcgcttt tcgcgctcga tccaagctgt
    20881 ttttgccata ggggcggcca aagtgcccca tccgaccccg cgcgcaagag aaaatttcca
    20941 ctggcaaagc ccggcatcct atcctatcac aaacccatta tgctgtttgg aatgttcatc
    21001 gccgccggtt ttctgctcct gggtgtcgtc gttttcacct ttttacagaa ggcctgagtc
    21061 ggcgtgccgg gatcaacccg ctgattcgag gcccatgtca ggcttggaaa taccggcata
    21121 caagcctgga aatcggcgct ccagggcttt gggcgtgacg tagccgatga gctgggcctc
    21181 gttccaatcc accagacccc agtaggcatg cggcagggcc atgatgacgg ctgtgcaggt
    21241 gggcatgcgc ggcacatgcg cgcgggccag gatgcgctcg gcgaactcgt gcatccccgg
    21301 attgtgcccg aacaacacgg catgagcctt gtcctcctcc agctcgcgca cgatctccag
    21361 caaccgctga gccggcgcca gatacatccg cgagtccagc cgcagcgcct ccaccggcaa
    21421 ggcgaagtgc tccctcatga tctgggctgt ggtcaatgcg cgcagggcgg tgctggtcac
    21481 cagcaaatcg ggcgaaggca gcagtggggg tgtctccccg ccaccgaaat aagtgtggcg
    21541 cagaaaagac ccgaccgcag gcgccgcgcg cagaccgcgc tcgttcagcg gacgatcatg
    21601 atccgccaag ccggattgct cccagcttga tttggcatga cggatgacgg tcaggtattt
    21661 catgaaaaag cgccgtccgt cgcatgacga ggggcgggtg tcaaaccatc tgcagacgcg
    21721 gtcatgacgg gaaaaaagtt cgggtaagga cggccgggca tcgaatcgcc cttgctcaaa
    21781 atgccatgag attctagttt atctagtaag taatgattat ttgtctcgaa acatccatcc
    21841 tcggtggtca cctttccaat gaagtccctg gtctgatcac gggatccctg catctggccg
    21901 gaagcagaca gccagtccaa gtcgagttgg cgggcaattt ttgccgggac atcgccggct
    21961 gccgtgtgga cctgcacaac ccgctgcccg acgcggacct ggatgacgtg gcatgggtgt
    22021 cacctctgca atcgggcttt tcaggtgtca tgacagcctc ttatcgagtg gtcaaaatgc
    22081 cccgtcggcg cagtgccgga ggcaaaaaac tggaggcgcc cgccggtctg aaaaatctcc
    22141 tgttcatgga atggttcaac aagcaaggcc agcggctgct catccagtcc tggcagatcc
    22201 aggtccgcgt gggcgccccg tgctggaaaa tgagcgcgga ggaggagtct gccatgcttc
    22261 gtcaggcgcg gacccggcga aagcacttcc tgctcaacca gcgcgaagac ggcggctcgc
    22321 aggatgcgtt gctggccccc ggactggctg acccctttga accccacgac ctgcgtggta
    22381 atccctttga caccctaccg gagaatctgc ctacccggga aaaagagtcg aaaagtggag
    22441 gtatagaccc cgcagcccga gccgcgacac tgcatgagga gttgcgccgg ttcggaaacc
    22501 ttctggatcc tgtcgtgact ccatcagttc gccccgcgat gatccagctt ctctccactg
    22561 tggccgacct tgccgtccac ctcatgcatg cgttgaggca tttcaagccc ggacatcccg
    22621 tgcatggcag gcacctggtc gtggacctgg agcaatccct gccgctcttc ggggcggcgc
    22681 tcaactccgt ggacaaactg gcgtccacac cctccatgga ggcgcagcgg caatggctcg
    22741 cgcgggtgca ggccggcctg ctgggcatcg aactgcggat gcgcgagctg ctcagtttga
    22801 tcagataagc ttttgcgccc agccaccggc tgctttgggc acggtcgggt cctcgctcgg
    22861 agcccgccat gcactgggca gggcgcgcac catgactcgg cttggttgac ccagggaaca
    22921 atcagcgtag atgaggccgc catgatccat ctccgcccac gtcatgtctc atgcctgctg
    22981 tgcagcatgg cactggcctc atgcgcgctg ctgccgcctc tgggcagccg gcgcatggcg
    23041 gaggatgtga gctcgttaaa ggggaaagct ccagaaaaat ggtccgtgct gccctccctg
    23101 ccggaaagag aatccaccgg ctggctggcc gatttcaatt cgcccaaact ccagtcgctc
    23161 gtatccaggg ccatccaggc gaacccggat ttgaaggccg ccgcagcccg tgtccgccag
    23221 gcgagggcgc aaaccatgga ggcgggcgcg gcccttttcc cggccgtttc cggcagcttt
    23281 aacgccgtgc gcagccaaac cccggccgac cagcgtttct cagggctggc cttcatcaac
    23341 aaccgcttcc gcctgccgct caacgtctcc tgggaggcgg acttctgggg tcgcgtggcc
    23401 gacgagcgcc gcgccgcgaa agccggcgcc caggcggctg aggagtccta ccatgcggca
    23461 cgcctttccc tcgccgccag cacagtcaat gccgccatca ccctggcgga agcccgctcc
    23521 caggcaaacc tggccgcgca ggatgtccgg acgcgccagg tgcagctcgc catcctcgaa
    23581 aagcagctcg accgcggtct tgatcccgaa cgcgccgcgc tcgacctctc cctaagccgg
    23641 gccgatctgg cacgcgccga gtccgccttt cagcaaagcc gcgcatcggc tgaccaagcc
    23701 gcccgggcgc tcgaaaccct gctcgggggc tatccggcag ggacagaacc aggcctcgac
    23761 accctgcctg ccctaaagcg gaatgtgccc gccgggctgc cctcggagat gcttctgcgc
    23821 cggcccgaca ttcgcgcggc cgagcgccgc ctggcagccg cgctgggcac ggaaagcgcg
    23881 gccaaaaaag ctttcctccc cagcttccgc atgacgggtg attatggccg caccacccag
    23941 tttctcgaca ccctgctcac tcctgagaca gctttctgga gcatcgcggg cagcaccacg
    24001 caatccttct tccagggcgg gcgcgttctc gccgccgcgc gcgcagcccg cgcccgttac
    24061 gatgagctgc ttcacgccta tgaggccggc gcattgaccg cttttcgcga agtcgaaacc
    24121 gcgctggcgg cggagcagtt cctcaacgac caagccgcgg ccctctcccg cgccgccagc
    24181 gaggccgaaa cagccgagcg cctcgcactc ggccagtatg agaggggcct cactccagca
    24241 cttacacttc tcgacgcccg ccagcgcgcc tttgacgcgc gcagcgccct gctcgccatc
    24301 caggcccgga gcctgcgcaa ccgcgccgac ctccatctgg ctctcggtgg cgaattttaa
    24361 tccagtcgtg gatgccggat agccgccggt ggcttttgcc cggcaaggac gcgccgcgcg
    24421 ccgcgtcttt catttcatgc gccagttcct cgcactcctt cttctttgcg gttccattac
    24481 ccaggcccag ccgccaaacc tcctcatcat cctcgccgac gactgcactt acaacgacct
    24541 cccggtctat ggcggacaaa acgcgcgcac tccgaacctc gaccgcctcg ccgccgacgg
    24601 actgaccttt aacaaagcct acgtcaccgc cgccatgtgc cagccctgcc gcgcggagct
    24661 ttacaccggc cagcatccca tgcgcaacgg ctgcgcctgg aaccacagcg ccagccgccc
    24721 gggcacgttg agcctgccgc agcacctcgc ccctgcgggt taccgcaccg gcatcgcggg
    24781 caaggtgcat gtgcagcccg cggagagcta cccgtttgaa aacgtcaccg gattcgaccc
    24841 caactgcgtc cgctcgccca cggaagccca cgatctcgcc ggtgtccgtg acttcatcac
    24901 ccgcgacaaa aaacagccct tctgcctcgt cgtcgccctc accgagccgc atgtgccctg
    24961 ggtcatgggc gatgcctcgg agtatccgcc caaaaaactc aagctgccgc ccaacatcgc
    25021 cgacacaccg aagacgcggc aggacttcgc cgcttacctc gccgagatca cctacatgga
    25081 cgggcaagtc ggcgaaatcc tcgccaccct ggagcaaagc ggggccgcgc aggacacgct
    25141 cgtccttttc agcagcgagc agggatcaca attccccggc aacaaatgga cctgctggga
    25201 caccggtctg cacaccgccc tcatcgcccg ctggccggga aaaatcgcgc ccggcactcg
    25261 caccgatgca ctcgtgcagt atgccgatgt ggcgcccacc ctgctcgacc tcgccggtct
    25321 gaaaacaaat cacccctttg acggccaaag tttcgccgcc gtgctgcgcg gagaaaaaaa
    25381 cacgcaccgc gacttcgcct acggcgccca caacaacatt cccgaaggca acgcctaccc
    25441 tgtgcgcacc gtcagcgacg gcgagtggcg ctacatccgc aacctcgcgc ccgacgagat
    25501 ttacatcgag aagcatctca tgggccagct cggcggcagc gccgcgcaca acgcctactg
    25561 gcccacctgg atggccaccg ccttcgacca tccgcgcact tatcagctcg tgaagcgcta
    25621 cctccgccgg cccgccgagg agctatatca caccgcctcc gatcccggcg aaatgaaaaa
    25681 cctcgccggc agcgccgagc acgccgccat ccaggccaaa ctcagcgccg agcttgaccg
    25741 ctggctcgcc gagcagggcg atcccggcct gccgcaggac acccatgaaa gccatcaggc
    25801 cgccaaacgc ggcgagcatc gtttcttccc gaagccctga gccagtcgct tcttcgaaga
    25861 gaatcactgc acaacccggc ggcggtcatg cgttcatcca gcgcatgaaa atctgcctcg
    25921 cactcctgct tcttgtctct ccggccctcg cgctcgaccc cgccaagctc gccgccattc
    25981 atccggcgat ggaggaggcg gtgaaggcga agcaggccgc tggcatcgtc acgctggtga
    26041 tggagaaggg caaaatcgtc caccatgatg ccgctggcat ggctgacatc gccaaagggc
    26101 gtgcgatgga gaaggacgcg ctgttctgga tcgcctcgat gacgaaatcg gtgaacggcg
    26161 cggcggtgat gacgctggtg gatgaaggaa agctcgcgct cgacgaaccg gcgtcgacat
    26221 ggctgccgga gcaggcgaat gtgaccgtcg agggcggcgc gaagccggag aaacccatca
    26281 cgctgcgcat gctgctctca cacacggcag ggatcgcctt ccctccgcgc aagccgaccg
    26341 atggcgcgat ctcgctcaaa tcgtacgtcg ccactctcat caagacgcct ttggccttcc
    26401 agccgggcag cagctacgaa tacggcttcg gccccacggt ggccggacgc atcatcgagc
    26461 tcgcctgcag catgaaatac gaggactacc tgcgcacacg cctcttcgag ccgctcggca
    26521 tgaaggacac caccttccat ccaaatgacg cgcagcgcgc gcgaatcgcc cgcacttaca
    26581 agatggacga ggacacgcgc gagctcgtgc ccggctacaa tcccttcgtc accagcgatg
    26641 cgagcgtgca gcacatgccg gagcctgcgg gcggcctgtt ctccaccgcg caggacatgg
    26701 gccgcttcta cgcgatgatc gccaacaacg gcgaactcga cggcaaacgc atcttgagcg
    26761 ccaaatccgt ggccgacatg acaaagcccg tcaccgccgg tgaaaaacag ctcaactacg
    26821 cctgcggctg gcagtgcaac atcgaaaccc agcgcgtgtg cgcggccatg cccgtcggca
    26881 gcttcggtca cggcggtgct tttgcgacga acggcttcgt tgatcccaaa agcggcatcg
    26941 tcaccgtctt catggtgcag aacgttctcg tgcccgatag cggcaagccg cgcgatgtct
    27001 tccaaaagct cgtcatggat gccgcgggca tcacggtgaa accgccgctc atggcgaaga
    27061 agaagcaaaa ataacctgtc aggttttgct tccaatacgt caggcagctg tgtcacgctg
    27121 tgcccgcctg cgatgaccgc ccgcaactcc ctacaccccg ccgcattccg caccacacgc
    27181 tggacctgcg tgtgcaaggc gcagtctgga tccgaagacg gtcgcacagc actcgccgaa
    27241 ctctgcgacg catattatga gcccgtgctc gcctttctgc gctgcgaact gcgggacgcg
    27301 gatgccgcac gcgacatgag ccacgctttc tttgccgaga tgctcggcag cggctcgatc
    27361 ggcggcgcag accgcacacg cggacggttt cggtcctatc tgctgggcgc ggtgaagcat
    27421 ttcgtggcgc gccagcgcga atctgcggcc cgcatgaagc gcggcggtgg ccttgaagcg
    27481 ctttccttgg atcacgagga agcggttgaa gtcgtcgata cacgccaagg cacaccggat
    27541 gcggagttcg accgccaatg ggccgtcacc gtgctcgctc gggggctcga agctttgcac
    27601 ctcgagtgcc ttgcagaaaa ccgcgagacg ttcttcgcat ccgtgaagcc attgctcatg
    27661 ggggatggcg cttacggtga tcaagccgcc cttgcagcga tctgcggcat gaatctggat
    27721 gcgtttcgca tggcggtgca tcgtttgaaa aaacgcctgc gacagtgcgt gaaggcggag
    27781 gtcgccggca cgctggatga ccccgcaatg gtgcaggagg agatgcaaag tctcttcgcc
    27841 gcgctcggcg gctgaaaaaa gtttgttatg gttttcccgc cacgcatcag gacaccggca
    27901 tgagcgaatc ctcctccaaa caatgcccgc aatgcggcgc tgacgtgccg gttagcacga
    27961 atggcctttg cccgcgctgc ctgatggccc agattgtcga accgacgcac gacggcggtc
    28021 caaacaacgc cgcgctgcgg ccgctggctc cagaggaact cgcaccccat tttccgcagc
    28081 tcgaaattct cgaatgcctc ggccgtggcg gcatgggcgt cgtgtacaag gcgcggcaga
    28141 aatcgctgaa ccgcctcgtc gcgctcaaac tgctcgcacc ggagcgcgcg gacgatccgc
    28201 agttcgccgc acgctttgaa aaggaggcac aggcgctcgc cgcgctgaat catccgcaca
    28261 tcgtcgctgt gcatgacttt ggacaggcag gcggattcta ctatctgctc atggagttcg
    28321 tcgatggcgt caatttgcgt cagctcctgc aaacaaagaa gctcacgccg aaggaggcgc
    28381 tcagcatcgt gccgcccgtg tgcgacgcgc tgcaatgcgc gcacgaccac ggcatcgtgc
    28441 atcgcgacat caagccggag aacctgctca tcgacaagac gggcgtggtg aagatcgccg
    28501 acttcggcat cgcgaagatt tatagcagcc ctctcgctcc gcgtgaggag caaagcactc
    28561 cctctcacga tgcggcaacg atgcctttcg gcactcccga ttacgccgcc cccgagcaag
    28621 ccaacggcgc cgccgaccac cgcgccgaca tctacagcct cggtgtggtg ctctacgaaa
    28681 tgctcgccgg cgagcgaccc aaggaaaaca tcaccccacc ctcgaagcgc gtgcaggtgg
    28741 acatccgcat cgatgagatc gtcttgcgtg cgctcgagaa ggctcctgag ctgcgttttg
    28801 ccaccgcggc ggagttccgc actcaggtgg aggccgcgat tcatggagag cctgctcaca
    28861 cccccaacgt ggaatgcatc gtttgtcgca cctgcggcca tgcgcggtct ctggacgtaa
    28921 cgcgcggcat ccagtggagc gccagtcaga aggaacgccg catgttcatg tggtgtccgc
    28981 agtgcggctg ccctcgggtg ggtgagcgca agatgggtgt gccatccaat gcgccttcga
    29041 tgccgcagat agttgagagc agttcgaaga tcaccttcgc agtgatgatc accctgttct
    29101 acgcgggtgc ggcattggta tcacttctca gcatgatgag ggtgggcgtg ctggaggcgc
    29161 tgatcgcagg agcaatcatg gtggtcgtgc tttggaaact cggattgctg ccgaaggtgt
    29221 cccacgcggg ctgccggagg ggtctttcct ggacggcctt tgcgctggcg ctgccaatgc
    29281 tggcgatggg catgttcttc gtctcgcaaa tccccgccga gacgggtggt tggaatccag
    29341 gacctgcaga actggtgttc gtacccctga cctggctcgg gatgctggca ctgccgtggg
    29401 ccggtgccgt gctctggcat tccaccagtc gccccgtcac acatcgaact gcggcgcgtc
    29461 ggcctgggtg ccttaccatc tttctcctcg tcgctctggc agtggcgatt cttgccggag
    29521 tgatgatcac catgtggagg ctgtccggcg cggcgtctcc ccggcttgag gcctcatgcg
    29581 tcattcgtga ggtgcgggac aatgtgatca ttctcgacac ggatgtgatc gcttcggaag
    29641 gaacggcgca ggtgggtttc tcgtttgaag gcccggcctt gtccgccgac cagttggagc
    29701 aggcgcgccg aaaagtggcg gatcgcaaaa tcggagtcat cggccctcat ccagatcccc
    29761 atccgaggat ggtccagggc ctgctggcaa atcacgatcg catgagcatc ggactcgcct
    29821 ttcccaccgc ggaagccgcc gagagcgccc gcgcagcaat gaaaagtccc gccgaggtgc
    29881 agattcagcc ggagaagcat gtgcaggcgc acaagatctt gttttcgatc accgacaacg
    29941 cgggacagag gatcatcggt tcgatgttcc tcggcaggca atcgctgggc catggcgcga
    30001 tgatcgatcc gcgcgggatg atttctttca ccacggatgc ggtgaacgtg ccgccggtca
    30061 ggccaggcag cagcagcgat gagttgaaaa agctccagac ggaactcgga cgtctgctta
    30121 ccagccgtgg ggagcagcat cctgaggtgc tggagttgcg cgcgcgcatt cgcagcctcc
    30181 agacgccggc tgcggtcggg gacatcgtcg gcatcggcgt cgctctgcat gtgaaaaatg
    30241 gccgcttcgt ggtggcgcag atttttcccg gctctcccgc agaatcgctg ctgaagcctg
    30301 gcgatgaaat cctgcgcgtg ggtgaatcgg acgacacatt gaaagacatc agcgcgatga
    30361 gcatcgagca agtcgtggac atgatccgtg ggaatgcagg cacgcaggtt ttgatcgtgg
    30421 tgaggtctga gggcgaagac gaacggaagg tcacccttgt gcgggaaagc cttggtgccg
    30481 ccccagtgac aactacactt ggaggcgacc agaaacctgc ctccaccatg actcaggaac
    30541 ccaacacctg gatcgttgaa ggccgcgtgg tgaatgatgc gggcaaaccg gtggcggagg
    30601 cgaacatcca tgtgagctgc ggcgtgggct cgctgcatac cacaggtgaa gggaaaacgg
    30661 gcgctgacgg ccgctatcgc gtgacgtttg gtcctggctt cctggtgtcg atcaaggatc
    30721 gcgcgggatc gttgcaggcc gcgctcgtac atgccagaaa agacggctac acagagaaaa
    30781 atctctgcga gtcgggtaat ctgcaaatgg cgtgggaact gacaaaacaa caactcgctg
    30841 gcggctggca gccgggaccg tcgcggacgt ttttgcccgg aaaaccgctg acggtggatt
    30901 ttgtgcttgt gccagcggcg cagattcaag gcacgctgat cgacaagaac ggcaaaccgg
    30961 tcgcgaatca agagatcgtt atcacgggag atcgcatcgg ccccggtggc agcatctacg
    31021 gagccacgcg cacggattcg caagggcgct tcaagttcaa ggacgtgtcc acacagcacg
    31081 cgtggtcgtt cagcatcgac gacaggtccg gccacccaga acgtacaccg ccagaccgtt
    31141 ttgcgaagcc ggaaacgcat cgcatcacgt tggaaatgga tggaagcacc ttgaagcgcc
    31201 tcaatccccc gcccaccacc accctccaag gcacgatgaa tgacaatgac ctcaagcagc
    31261 tctcctggca cgagtttgac cagacgaagg gcaaatactg gcgcgaactg gccgatgtgc
    31321 ggcgcttcaa ggaggcagcg gagttgatcg agaggatgct ctcgctgcac ccggatct
//
LOCUS       DAOMYJ010000034        30838 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Prosthecobacter sp. isolate AMR_MDS_5629
            NODE_531_length_30838_cov_21.616866, whole genome shotgun sequence.
ACCESSION   DAOMYJ010000034 DAOMYJ010000000
VERSION     DAOMYJ010000034.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867850
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prosthecobacter sp. (wastewater metagenome)
  ORGANISM  Prosthecobacter sp.
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Verrucomicrobiaceae; Prosthecobacter.
REFERENCE   1  (bases 1 to 30838)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 30838)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.30
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:34:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,742
            CDSs (total)                      :: 4,684
            Genes (coding)                    :: 4,676
            CDSs (with protein)               :: 4,676
            Genes (RNA)                       :: 58
            tRNAs                             :: 55
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30838
                     /organism="Prosthecobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_531_length_30838_cov_21.616866"
                     /isolate="AMR_MDS_5629"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1965333"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            269..1270
                     /locus_tag="PLP58_05610"
     CDS             269..1270
                     /locus_tag="PLP58_05610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ08025.1"
                     /translation="MPLSEPDNEVPARRPVFALFLLGIVALVGAWLAWEEARPAWSAD
                     FPVAQPKKDEAGEQALEKLALSHAPADAGAPLVAESPPKPEAAMPSPPVTEPEIRRAE
                     MPGVPEKVALNPTPELLRQTGDLLSKFLAAGGPDERLALIATPEEHAVDVAEFFEPGP
                     PVVSSLAYANATPQSLPGGMLVPLFQVVTDRCRGGALLRLAPQEDGRLLLDWPLFAET
                     HGRRLAGFIEKRPDDPAWFHVGMRRSHALDLPAEFRGRHFGFDLQGGADGSVRCVAVA
                     ARETPLGRFFERETEWGVVYLARVLLQHRLDETAGRVIVILDCEGASTGASKAGPGQ"
     gene            1381..2802
                     /gene="rpoN"
                     /locus_tag="PLP58_05615"
     CDS             1381..2802
                     /gene="rpoN"
                     /locus_tag="PLP58_05615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009961414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HRJ08026.1"
                     /translation="MSTQGLYQTQSQKLAIGPQMQQSLQILQAPTLELRQIIQQEIEI
                     NPVLELETPEVSLEDAVPDDPDAAVGESDDLDMLSQMDEEWREYWAQSRMQNSNRTAE
                     DQERWQFLMDSIVTPTTLQEHLLGQLRTAEVTEPVLISNVEFLIGNLDDRGFLNQSVE
                     DMSLYQGIPVDDLHAAKAVLTTFDPVGVGAEDLRECLLIQLERLGKSHGLAHRLVEQH
                     LDDLAGKRYPILARKLGVPLEQISRAADFISTLDPRPASRFVQGGNSYVSPDIIVERQ
                     GGEWVPVMNNDDLPQLRLSNAYKDMLSQTGSSGEVRNYIREKLRSGKFLIRSIHQRQQ
                     TIHKIAAQILAHQREFLEKGTSHLRPLNMAQVADEVGVHETTVSRAIAGKYMATPHGV
                     YELKFFFSHGVKTESGEDLSNTSVKNAIADLVKTEPKNKPLSDDKITKLLDRQGIKVA
                     RRTVAKYREALGILPSHLRKSFS"
     gene            2901..2986
                     /locus_tag="PLP58_05620"
     tRNA            2901..2986
                     /locus_tag="PLP58_05620"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2935..2937,aa:Tyr,seq:gta)
     gene            3193..3969
                     /locus_tag="PLP58_05625"
     CDS             3193..3969
                     /locus_tag="PLP58_05625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ08027.1"
                     /translation="MMESTAISNLCEACESRPVVVAEACDDEDQPYRLCEGCQHRLVH
                     RSLRPMEWYRLALRHCPHNFSLHDDFYLDDGTADQPKEDVVDAHLLPAPSLADKAGSL
                     KDLWEFTLTRYALREELLEAWRKFPASDMLEWLTTAYDSREDCHCRSTVLELAEKCLG
                     LAGADLVRRAWLDYPDKMHFAGLANASVACLPEEDGFERCFAVFDALPLEEQRSRMMY
                     MRKFRNPQMLDWIETHIQSPVTDVFRAGGLLQIARPLQRA"
     gene            3914..4477
                     /locus_tag="PLP58_05630"
     CDS             3914..4477
                     /locus_tag="PLP58_05630"
                     /inference="COORDINATES: protein motif:HMM:NF015422.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helicase associated domain-containing protein"
                     /protein_id="HRJ08028.1"
                     /translation="MFSELVDFFKLHGHCNVPEDWTAQPELARWVTKQRRAKKQNLLT
                     LEQVSRMEDIGFAWSTHDGGWDAMFAKLVEHMRPMHNGKPRETPPSDELKGWVLTQRQ
                     FKKRGDLDPEREKRLASIGFEWEPFSAQWEAMFARLRAYHAERGNCLVPSKWPKDQQL
                     ASWVGTQRMRKSGGASCQQNESRSSTR"
     gene            4800..4955
                     /locus_tag="PLP58_05635"
                     /pseudo
     CDS             4800..4955
                     /locus_tag="PLP58_05635"
                     /inference="COORDINATES: protein motif:HMM:NF015422.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helicase associated domain-containing protein"
     gene            5007..5420
                     /locus_tag="PLP58_05640"
     CDS             5007..5420
                     /locus_tag="PLP58_05640"
                     /inference="COORDINATES: protein motif:HMM:NF015422.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helicase associated domain-containing protein"
                     /protein_id="HRJ08029.1"
                     /translation="MFQQLVEFKKEHGHTNVPQRSHRYAELATWVRNQRAAKAHNRLI
                     IAERGRRLDEIGFVWRLVERNAWERMFDRLVEFKKLHGHCNVPQKGGGDKLLGKWVNT
                     QRTHFKRGTLKPERVQQLKEVGFVWNTKVPRGESR"
     gene            5433..5771
                     /locus_tag="PLP58_05645"
     CDS             5433..5771
                     /locus_tag="PLP58_05645"
                     /inference="COORDINATES: protein motif:HMM:NF023295.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3387 domain-containing protein"
                     /protein_id="HRJ08030.1"
                     /translation="MQAIARVNRVFRDRPGGLVVDYLGLVDQLKHALANYTESGGKGD
                     PTYDTKQAIAVMLEKHGIACDMLHGSDWQKAVGDGKKTLFALPALQEYLLELEDGKTR
                     WNQVIEGNDE"
     gene            6293..6532
                     /locus_tag="PLP58_05650"
     CDS             6293..6532
                     /locus_tag="PLP58_05650"
                     /inference="COORDINATES: protein motif:HMM:NF023295.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3387 domain-containing protein"
                     /protein_id="HRJ08031.1"
                     /translation="MRPSLRAVCLARLPAARLYDALAANESAVKVMGDDKLKVIAAEL
                     ITQVRKSASIDWTLRESARAKIGSLAAAARSACGL"
     gene            6639..6746
                     /locus_tag="PLP58_05655"
     CDS             6639..6746
                     /locus_tag="PLP58_05655"
                     /inference="COORDINATES: protein motif:HMM:NF023295.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3387 domain-containing protein"
                     /protein_id="HRJ08032.1"
                     /translation="MVKRILNKYGYPPDLQEEAVKTVLAQAELLCAEWV"
     gene            complement(7057..7458)
                     /locus_tag="PLP58_05660"
     CDS             complement(7057..7458)
                     /locus_tag="PLP58_05660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009964086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor-like protein"
                     /protein_id="HRJ08033.1"
                     /translation="MPLPDDDALLKRMRKLRLREEDLEESFIRGGGPGGQKINKTSST
                     VVLRHVPSGIEVRCQRERSQVMNRHWARVELCERMEAALEQARLAIQEEREKKRRQNR
                     SRPRSVKREMLEGKRHRSGVKRNRGRVGGDD"
     gene            7558..9012
                     /locus_tag="PLP58_05665"
     CDS             7558..9012
                     /locus_tag="PLP58_05665"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HRJ08034.1"
                     /translation="MKTLILPFLCLAALFHATAAGRPNIIFVMADDMGWGQTGYRGHP
                     LLKTPNLDAMAANGLRFERFYAGNPVCSPTRASVLTGRSNDRAGVLSHGYALRLQEKT
                     IAQALKSAGYATAHFGKWHLNGYKGPGAPVLKDDPRSPGAFGFDEWVSATNFIDMDPW
                     LGRMGAPEQITGDSSEVIVAEASKFIERKKDGPFFAIIWYGTPHSPFRALEADKAPFA
                     HLDEASANHHGELVAMDRSIGALRKKLRDLGIADNTLLVFNSDNGGLAGIQPGTTGGL
                     RGNKGTVYEGGLRVPGIMEWPAVIKPGRVTSFPASTMDLFPTVADIAGLPETCFIQPV
                     DGASLKPLLAGTESGPRVKPIPFRYGRKAALVDNMHKILTDNLDGGAFALYDLESDPH
                     ETRDLSADMPELFEKMKKDLLAWSAGVDASFAGKDYPEGAVSPADPVPQNWHEAEPYL
                     KLAGLWKGYWAYDSHINRQAKGAAKPGAKKKRQP"
     gene            complement(9038..9871)
                     /locus_tag="PLP58_05670"
     CDS             complement(9038..9871)
                     /locus_tag="PLP58_05670"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HRJ08035.1"
                     /translation="MSGGKAAPIARRSLFAAAALALSGFQQVSAQSSGQVRCQLADGF
                     DFPVGKPNADGYYKSRGYWPNGHLGEDWNHRSGGNNDLGHPIYSCARGVVVISEDVRA
                     GWGNCVIVRHAYRDATGKIAMVDSLYAHLHQRGVKVGQVVEKGQLVGTMGGNNGMYYV
                     HLHFEMRKNLRIGMNRSQFARDNSNYYSPTDFINKHRTLPTSFQKYPIPLGYFAPYGR
                     SQADMRPDDGAVIQVPTLPGGASKILPESGPSADDEDFWSKLRNRLKQGKVTNGLDDQ
                     R"
     gene            complement(9901..10515)
                     /gene="secG"
                     /locus_tag="PLP58_05675"
     CDS             complement(9901..10515)
                     /gene="secG"
                     /locus_tag="PLP58_05675"
                     /inference="COORDINATES: protein motif:HMM:TIGR00810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HRJ08036.1"
                     /translation="MDIVIGILMVVEVLVCLLLILIVLMQRPRQEGLGASFGGDMMSQ
                     IAGAQTTNVLQRFTVYLAIILFVLTLGLSMLTARQQTARANAKIFDEPPPPAPVEAPK
                     PVESPAPAAAGDAAPKAGEKSAAPAKDETAPAKKDEANPPAPPAGAPNPPAEAKNAPA
                     TPQAAEPAPKAATPAMKEPAEPAPPAPPAAADKPAAEPAAPKSN"
     gene            complement(10609..10851)
                     /locus_tag="PLP58_05680"
     CDS             complement(10609..10851)
                     /locus_tag="PLP58_05680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ08037.1"
                     /translation="MLLNAAIPVLCILWVLLCLRFYTLISHENVWQRRFIAIGAGLGA
                     GLIYLLGSMLMPWLKMPDKPLEAPVEQSENVRVKGK"
     gene            11023..14676
                     /locus_tag="PLP58_05685"
     CDS             11023..14676
                     /locus_tag="PLP58_05685"
                     /inference="COORDINATES: protein motif:HMM:NF013245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRJ08038.1"
                     /translation="MSTSQMSRPSLSRRPESQAREPAGLMRRLSPLWWWRRWFAEEDG
                     APVLASRDGNSMLPVLIEVFAGFSKLDGAVQEDEIESSLGYLRYDYSGTLYADLRRLY
                     FEALQEPQDLSKRASELAEELTLEQKILLCVQLYLLISRSLTDQRMMVEFYLFMTNLG
                     IAAQAIDIVYQLNSGDKPQDEEFSGKTGQPLETLRIGSGGGCDVLLRTLSTDCSVMAF
                     RYQNLLLLKNTGAIPVLVRSRRLPPGEFSRLYPAERVVLEDVTLDYNDLASYFSAKKT
                     VTGAQLYLTLTETGDAEIAPQRARGTNLRVSFGLGVSIEALRNTDATLNGVLLKEGTR
                     VEASIEDSIIAHGEIEISLRDLRVRAQEFGGQFRLEGSRNTYLVSNNPELLDEGDILL
                     SDDTEGEILLRIECNYAQKTGTLDLLRASRPIYVGQVPVRDRISLSDGDTITLGEGQF
                     LRCHFADRIIEEEKNTIRQMEVRELAHRFDSKDTALDGISFSARRGEMICVMGPSGCG
                     KSTLLRALGGQLRTRGGDILMNGLSLYQNLPNLTPYIAYIPQDDAFDPLLKVQENLDF
                     SVAVRCPHLKTEERRKRVEAKLAELGLADMRKRLAGTPQQKFLSGGERKRLNAGLDMI
                     GISDIYLFDEPTSGLSSKDSEHVLEIIRSLARNKLVLCSIHQPSMKLLQMFDKALLLD
                     KGGKMAFFGTPQAMLEYFWRAYNDETGQPGAAMPANLTPDFVFDVLETPLRDISGDII
                     QERSADGHLVAARRFPPNFWRDRYQSHLLLESMAKAQAAPGSTNLIARPREDTQSGSR
                     GRAMPKPPKHTVREESILFYTMLKRAFLSKLRNRTNLLTTLLEAPLLAFLISSVLKYS
                     EEDAYTFATAFHIPTYLFMSLVVAMFLGLTNSADEIIRDRHLLSRERNHNLRTFYYLL
                     GKILSLSAFALVQCVIYLFIGNWVLEIRDMFFTHLWWMFVTALTGACLGLLISSFVGD
                     SRTAINAIPLLLIPQIILGGALIKYEEMNRNLDLIYSLQPWLVKAGIYEEETKTKLKV
                     PFICQFMPLRWSYEGMLISQAKLNPLTHAQDTLEERIQELVSIPPEIKLTNDQQKELD
                     AAKQGLAVVSGLYAKDSDAAAKMLARIWDATLRGRLTPELLKEALEQQDGTSAEEIYV
                     NRKVLDLVTKAEMERTDYRRKASPNVFFGTSKTYFGTPFKTLWINSMVILITLGLIIT
                     CVEFSLRRQLTRV"
     gene            complement(14706..14876)
                     /locus_tag="PLP58_05690"
     CDS             complement(14706..14876)
                     /locus_tag="PLP58_05690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ08039.1"
                     /translation="MKLTDLRQNPCEAAHSSQAAIARIFFKTGPGESGEGDHFPGLKV
                     PQSGGGLVKRKP"
     gene            complement(15194..17071)
                     /locus_tag="PLP58_05695"
     CDS             complement(15194..17071)
                     /locus_tag="PLP58_05695"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HRJ08040.1"
                     /translation="MTQTQKSLRALGAAGIAWTCACLGHGAETPAEAGTNKPLPPVVV
                     TATKTETEAARTASSITVIDRAKIEEKQYRQLVDALREVPGLTLADRGAPGTVTGVFI
                     RGTKTEHTRVLMDGRPVPANLAGAYNLETTPLDNIERIEVLRGPAAGIYGGKTIGGVI
                     NIITRSGRGLEKPETSVFFETGSYGTFREGISTLSAAGDLDWSFEAARADVQGQRVNS
                     RYGQTSANGRIGWQIAETLRFELDGRWYEAEVGLPGSRFANDRDDNLLTEFWSISPRV
                     IWDTTDGWRQSLTYSFNNFRQVASGFTGFFTPNNRVSIRHHFLEYQSEFRPFDIWTIT
                     TGLWLEDQNLTRFNDNIRSLDIDQNETNWAWFLQSQLEVLPGLDFISGLRYDRYSDFD
                     DAVTWRAGLAWRVPVLQTLLHGNYGTAFSPPSGQDREPALFGNPQLARPERSRGWEIG
                     IEQPIPAAKTTLSATWFRNELKDTYQFDLGTFSLQPIGRARTQGVELGVLWEPHEILS
                     FNAAYTYLDADDLGAGARLVRRPRHNLSGGVSLRPHDKVTVNLSALYIMDREDFDPAT
                     FAQKDLEDYLLARLTVNWRVCRNFEVFARVENLLGDNYEDAAGFPAFDTGAYAGIRLK
                     F"
     gene            complement(17575..18756)
                     /locus_tag="PLP58_05700"
     CDS             complement(17575..18756)
                     /locus_tag="PLP58_05700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009961509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HRJ08041.1"
                     /translation="MDYDSKLCTQIRDLPRSGIRDFFELVIGRSDVISLGVGEPDKPT
                     PWPIREAAIRSLEKGVTSYTSNLGLESLRVAISDYVEKQFRVTYDPKTEILVTVGVSE
                     ALDLAFRALLNPGDEVIYHEPCYVSYSPGIKMAYGVPVVVETREENKFALMAEDVERV
                     ITPRSRVLALNFPTNPTGGIMPPEELEKIARLAIKHDLIVITDEIYCELLYDGRKHTS
                     IVEFPGMKERTILLHGFSKAFAMTGWRLGYACAPAPLREAMMKIHQYCMLCAPIMSQM
                     AGIEALKMGANAYEEMRQSYEQRRNLIVSRLNGMGLHCFNPGGAFYVFPDIRPTGLGS
                     KDFAIRLLEKKSVAVVPGSAFGAAGEGFVRCCYATAPDLIVKAMDLMEEFVGEARSAG
                     L"
     gene            complement(18772..19251)
                     /locus_tag="PLP58_05705"
     CDS             complement(18772..19251)
                     /locus_tag="PLP58_05705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012420802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HRJ08042.1"
                     /translation="MDPLIQLLQINSNRSAHELAQILGLTEAEVITRMQAAEADGTIL
                     GYNAVVDRHKAGHRGVMALIEVRITPEREGGFDRLARRIAKFDQVRECFLMSGGYDLA
                     VIVEGKDLLDVANFVAEKLSTLGGVLSTATHFQLKAYKQSGFLVNSANEEERLPVSP"
     gene            complement(19328..20290)
                     /locus_tag="PLP58_05710"
     CDS             complement(19328..20290)
                     /locus_tag="PLP58_05710"
                     /EC_number="1.2.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009962285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HRJ08043.1"
                     /translation="MSITYLEAIREAQYEALRTNPDVFLYGQDISTFGGAFKATKNLS
                     REFPGRVFDAPISEDAMVGMAVGAAIEGARPILEMQFADFSSVGFNQIVNQAATLYWR
                     TQTPCPITIRLPSGGTAGSGPFHSQSMESIYAHYPGLVILTPATVEDAYWMLLDSVEL
                     DDPVIFCEHKFLYYHLKAEDFGQRVPIGKARIARPGRDATVVTYSAMLHESLRAAEEL
                     QRDGYQIEVVDLRSVKPMDTDTILASVSRTGRLLAVGETFPWGGVTAEVIARVTSEAF
                     HMLDAPPMRLNSKDTPIPYHPNLWKSHRPTTGSISNALRELLRY"
     gene            complement(20287..21324)
                     /locus_tag="PLP58_05715"
     CDS             complement(20287..21324)
                     /locus_tag="PLP58_05715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009962283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent dehydrogenase
                     E1 component subunit alpha"
                     /protein_id="HRJ08044.1"
                     /translation="MPAAPSPSETLMPVDLRDDFLRALRCMILSRTLEERLGSLYRAG
                     GRIVGGVYLGRGQEAFSASAGIHLRKGSDIYGPLIRDQAGRIAYGEPVLDTLRTYLGT
                     VTGPMRGRDGNIHRGKPREGYMAMISHLGSLVAVVAGALFARRLKGVLGDSVGATSIG
                     DGGTSTGAFHEGLNLAAVEKLPLIVSVANNQFAYSTPTARQYACEHLADRAAGYGVEG
                     YSVDGTDLYQTLAVFRTAFARARGGHGPQLVVGALLRLGGHGEHDDASYIPDVVKHQH
                     EGRDCLVVARQRALDEGWADEKIFREWEEEARREVDAAQAQAGREPAPDPYRETWQAL
                     ATASLVEGQNP"
     gene            complement(21439..22347)
                     /locus_tag="PLP58_05720"
     CDS             complement(21439..22347)
                     /locus_tag="PLP58_05720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009964936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HRJ08045.1"
                     /translation="MKPTLLILAAGMGSRYGGLKQLDAMGPSGEVVLDYSVFDAIRAG
                     FGKVVFVIRRDFEDQFRAQIGGKYGGRIEVEYAFQDLNDLPEGFTLPEGRTKPWGTAH
                     AVLAAESVVREPFLMINADDFYGQDAFAKIAADLTAPRAQDGRSHYSMVGFYLKNTLS
                     EHGSVARGVCTRGADGMLASVTEMTKIFKTPSGAENRETEPPLPLTGGEVVSMNFFGF
                     TPDIFGHLRAAFVEFLNANAGDLKAECYVPKVVDSLIQAGKADVRVLESNDSWFGVTY
                     PEDKADVVASIRALVAAGRYPENLWA"
     gene            22484..23791
                     /locus_tag="PLP58_05725"
     CDS             22484..23791
                     /locus_tag="PLP58_05725"
                     /inference="COORDINATES: protein motif:HMM:NF012311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HRJ08046.1"
                     /translation="MQNGLKILIVLSALVCAAALVMLGGLALETTAPDRPVKISKPLT
                     NWGAGDRRGSGPDFSLPPPDAAVDDLAELMRKLLELSRSPGVEPDELLLSFKSRAALD
                     AFRARAARDGIEVLYSDPRLLTARVRYGDTAAMARELRDNAGAYDNIGMNFRAWVPAP
                     QRVPETDTANAGGMRPYGDSGLDAIGAAGDRATWGKGVTVAVLDTGITPHPVLEHSSI
                     SRTDFVQDGKELDGHGTAMASLIAGEDPQNGGVAPASRLLDIRVANGNGESNTALVAQ
                     GILAAVDQGARVINISLGTNADSLTLRRAVEYAIAQGVIVVAAAGNDQSKALAYPAGY
                     TGVVSVAAVDANGVQAFFSNSGPGLFISAPGVGIVSAYSGDGMVISSGTSQATALTSG
                     AVAAMLSRGYNTQNVMQALVNNARPTGLPATQTGAGILQVPRL"
     gene            complement(23812..30540)
                     /locus_tag="PLP58_05730"
     CDS             complement(23812..30540)
                     /locus_tag="PLP58_05730"
                     /inference="COORDINATES: protein motif:HMM:NF015140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007154 - cell communication [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Calx-beta domain-containing protein"
                     /protein_id="HRJ08047.1"
                     /translation="MRKPLILILAALACAAWLFVMLDKRAPRADQARALTAGAAGGGE
                     EVFSPVRDWPPAPPKWEEAAAPVLRDFSSWSRRYLEADDAGRAALVAEGVALARARRA
                     EFKRLIVEDPRAAVEGAVPMVVRQALPPEVVAELERRVHATADVEVLAVSEGSGPDEP
                     VVRWFTRFGGEELRTYVYGRRSGHNSLYGVPVHGVAVDEVLALAESPARVLEIGERPP
                     AGAKTVETCPVSGLSTAVENTQAPIVEETPAVQAGENIVYLCDGGHIRMFVEELVAGE
                     GGTGGAAGLSAPAASGWNNGPRKLLYMRVIFEDSLQEPQTEAQAWGAIEQLNDYFAEV
                     SFGKVFYLADVTPAILLPRSRAWYTADYTATGSNSPIMTDAKEAARALGYNPDNYQHF
                     TVIYTGGTGNGPGTFGGLGSVGGANVWLRTTSIGTFAHEIGHNVGLWHGNSWDSNRTI
                     GVSWNTSLTRDVIGGGLNGEYGHSYDVLGSSGSFTGNKGHFMAHHKNLMNWLPTETVA
                     AATTSGVYRIFPMDQTVLDPERRYALRVTKDAGRDYWVDFRQKYNADHRWFRDGVLLH
                     WSRWGQNQVGNEDQVVGGNRGGQLLDTTPGSADGKNDAPVVIGRTFSDRESGVHITPL
                     GKGGTTPESMDVQVHLGFFPGNNAPVIHSLTASTTTPALNGTVNFSVDATDPDGDPLA
                     YFWDFGDATYGVNAAATSKQFTTARHYAVRCIVSDMKGGETSKLVLITAGAPSTRTAS
                     GRVLDGLGNPVAEVRIQNGSTGTSFRGAWTDSNGDFVVTNLTSSSVLLTPVKGGHVFS
                     PVNINVPSGGSVTGMDYTANDAVLVSIEAVDAEAAETGGNTGLFRITRTGSTAADLTV
                     ITDLQGDATFNTDHTLAPAPNTTALTPLETFTLPAGASTLDITLTANNDSTREGPEAA
                     RIVLMPSNSYSITGAAAATVVIQDTGTNSVPNRVSVAAVDEDAHETGEAGVFEIRRTG
                     PVDASLNVSITLDGAPNTAAAANGQDYQTISSPVTIPAGESAVQVTVVPLQDTLIEGQ
                     ETVRLTVASNSAYGVGTPNSAIVRIADDDIPTVTLTATDAAASEAGNNTGTFTVTRTG
                     DTGAPLTLGYTAGGSALHGTDYLPLPGEVTIPAGQSSADILIVPIDDDHGEPNQTVVL
                     QLRADPRHVPGTPHRDTVTISDNDLPVVAVGVLDATCNEPSDAGSFRITTTGSGTGNI
                     TVRYTVSGTATPGADYTALTGTLSMAKNATATLTVTPINDSLQEDAESVVVTLLPDPA
                     YQVDVLQPEAMLVIRDDDSGSVVNASFSTLTMTESGNSKLFLSRTSSTGAATTSDNLT
                     VSYALSGTATPGVDYSGLSGMAVIPSGASNVSVDITGIDDSDAEGTESLIVTILPGSY
                     SRERATATLHITDNESAGFARTASFATRRTVKTEGDAPFTIPVVLSSPDPVNEVRVDY
                     ILDTNSATGSGVDVNLKAGSLVFPPGVSQVEIPVSIVDDLLPEPEEHVTLKLSFAHGA
                     QLTSGGSYHTLFIRDNEPRVSLRAAHPAAYESNGAPGAFTLSRTGPVNAPLEVSLAWS
                     GTATAGADYAAMPATASFAAGERHLELPVTPLPDALAEGTETVIATLNASSRYLVVGE
                     ASTTVDILDNATNHPPTVRITQPRRAEAGLSLSTGLHLGAAVTDDGAPGSTSVLWTHV
                     SGPGTATFGQASALETTVVFSAAGRHILRCTATDAGSLSAHAEVAVAVGGGGALWAAE
                     DIGITGTNATGSADIRSGWAQNQGAGSSITASSDSFHFQHTELSGDGEIVARYEGTDG
                     LFSNARTGVMLRDGTAANARCAGLTVLVTVSGGNITSTLAWTRRTTAGGAVSTTTASL
                     PHGPRWLKLARAGSNFSAWHSLDGIDWTQVGTAQAIALPATLRAGIATTSGNTARPAR
                     GLFSGIQVSGTPENAAPLSQAATTPSISAAAFRVLAGSVLDDAPDAPDAQWSVVSGPG
                     VVTFEDAAAAQTTAQFAAEGAYVLRLSADDGEASSFDDVAVDVQFATLGFGAAADAAE
                     EGAAPGLVTVTRNGQLDMPLTVHYTTGGSASAPGDYQALPGSVTIPAGESSAAIQVQP
                     VADDVAEGDKTLAINLQADATYHLPVEPVSVIQVKDRPMDAWRFAQFGAQANNPAVNG
                     LLMDPDNDGWNNLLEYALGLQGMEPDESPLAQDTATVGQDKFLRLTVPKNPDATGITY
                     TVEATSDVTDPLSWSSAGLVVEVDNATTLRVRDNVPAGPGVIRFLRARVTMP"
BASE COUNT         5485 a        10040 c         9844 g         5469 t
ORIGIN      
        1 gatgccggcc gtgtcgccct ttggattcgc ggctcctccg cgtgcggcgg agccggcccc
       61 ggcgcctgcg tttcaaggcg gcttcgcatc tccgccgcca ttttttcagc cgcactcccc
      121 tccacagccg gagcctccac agtggacctt cctcgatccg ttgccgtctg tggataacag
      181 cgcgcccctg ccgcaaccgt ccgcgccatt cgccgcctcg cctttccagc cggctgaaaa
      241 ctccgctcca cttttccagc ctgtgggcat gcccttgtcc gagccggaca atgaagttcc
      301 cgcgcgccgg cctgttttcg cgctgtttct cctgggcata gtggcattgg tcggcgcgtg
      361 gctggcatgg gaggaggcgc gcccggcgtg gtcggcggac tttcccgtgg cgcagccaaa
      421 gaaagacgaa gctggcgagc aggcgctgga gaaactcgcg ctcagtcacg cgcctgcgga
      481 tgccggtgct ccgctggtgg cggagtcccc gccgaagcct gaagccgcca tgccgtcccc
      541 cccggtgacg gagcccgaaa tccgccgggc ggaaatgcca ggcgtgccgg agaaggtggc
      601 attgaacccc acgccggagc tgttgcggca gacgggagat ttgctttcga agtttcttgc
      661 cgcgggtgga ccggatgagc ggctggcctt gatcgccaca cctgaggagc acgcggtgga
      721 tgtggcggag tttttcgagc cgggtccgcc cgtggtgtcc tcgcttgcgt atgccaacgc
      781 cactccccag agcctgcccg gaggaatgct ggtgccgctt ttccaggtcg tcaccgaccg
      841 ctgccgcggg ggcgctttac tgcgccttgc gccgcaggag gatggcaggc tgctgctgga
      901 ctggccgttg tttgccgaga cgcacgggcg caggctggcc ggcttcatcg aaaagcggcc
      961 tgatgacccg gcgtggttcc atgtcggaat gcggcgcagt cacgcattgg atctgccggc
     1021 ggagtttcgc gggcggcatt tcggtttcga tctccagggc ggcgccgacg gttccgtgcg
     1081 ttgcgtggcc gtggcagcca gggagacgcc gctgggccgg ttcttcgagc gcgagacgga
     1141 atggggcgtc gtttatctcg cgcgtgtcct gcttcagcac cgcctggatg agacggccgg
     1201 gcgggtgatc gtgatcctcg actgcgaggg ggccagcaca ggcgcgtcga aagcgggtcc
     1261 cggacagtga tgggtgccag gtttgcgcaa gtctttttaa aatgcggata aaatcgcagt
     1321 ttggcccggc ctttgcttgc caaggtgttc caccgatgaa aggggtggct tgaccaaccc
     1381 atgtcaaccc agggactcta ccagacacag tcgcagaagc tggccatcgg cccgcagatg
     1441 cagcagagcc tgcaaatctt gcaggccccc acactggagc tgcgccagat cattcagcag
     1501 gagatcgaga tcaatcccgt gctggagctg gagacgccgg aggtttccct ggaggatgcc
     1561 gtgcccgatg atcccgacgc tgccgtcggc gagagcgacg atctcgacat gctctctcaa
     1621 atggacgagg aatggcgcga atactgggcg cagagccgga tgcagaactc caaccgcacc
     1681 gccgaggacc aggagcgctg gcagttcctc atggattcga tcgtgacacc cacgacgctt
     1741 caggagcacc tgctcggcca gctccgcacc gccgaggtga cggagcccgt cctgataagc
     1801 aatgtcgagt tcctcattgg caatctcgac gaccgcggat tcctcaacca gtccgtcgag
     1861 gacatgagcc tttaccaggg catccccgtg gatgacctgc atgccgccaa agccgtgctg
     1921 acaactttcg atcccgtcgg cgtcggcgcc gaggatctgc gcgagtgtct tctcatccag
     1981 ctcgagcggc tgggcaaatc ccacggcctg gcccaccggc tcgtcgagca gcacctcgac
     2041 gacctggccg gcaagcgcta ccccatcctc gcgcgcaagc tcggcgtgcc gcttgagcag
     2101 atttcgcgcg cggcggactt catcagcacc ctcgatccgc gtccggcctc gcgctttgtc
     2161 cagggcggga acagctatgt cagccctgac atcatcgtcg aaaggcaggg cggagaatgg
     2221 gtgcccgtca tgaacaatga cgacctcccg cagctccgcc tcagcaacgc ctacaaggac
     2281 atgctcagcc agaccggcag cagcggcgag gtgcgcaact acatccgcga gaagctgcgc
     2341 agcggcaagt ttctcatccg ctccatccat cagaggcagc agaccattca caagattgcc
     2401 gcgcagattc tggcgcatca gcgcgagttc ctcgaaaaag gcaccagcca tctccgcccg
     2461 ctgaacatgg cccaggtggc cgacgaggtg ggcgtccacg agaccactgt gagccgcgcc
     2521 attgccggca aatacatggc caccccccac ggtgtctacg agttgaagtt ctttttcagc
     2581 cacggcgtga agaccgaaag cggcgaggat ctcagcaaca ccagcgtgaa aaacgccatt
     2641 gccgacctgg tcaagaccga gccaaaaaac aagccgctca gcgacgacaa aatcaccaag
     2701 ctgctcgaca ggcagggcat caaagtcgcg cgccgcaccg tcgccaaata ccgcgaggcc
     2761 ctcggcatcc ttcccagcca cctgcgcaaa tccttttcct gaatcacgga cgggcaaccg
     2821 acgagaaaaa tgtgacaaag ggcaaaacaa ccttgcaatc agaaggccgc cgcgtttact
     2881 cgccactcca ccaaaacaaa gggtaggtgc ccgagtggct aaagggggca gactgtaaat
     2941 ctgctggctt acgcctacgc tggttcgaat ccagccctgc ccaccacatt cattttcaat
     3001 gaatgtgcgc gccttgtcga aggcgcgatg gcatgtggga aaaattcacc actgtcattt
     3061 ttttgtcact tttgaggcca ccccctggtt taagcgaagg gtttccgatt gggttttgga
     3121 ttgggtatgg catttccggg cgtgtgaatt gggacgaagg gttgcaggcg tgcttggctg
     3181 catcagagtg gaatgatgga atcgacagcc atttcaaacc tctgcgaagc ttgtgaatcc
     3241 cggccagtcg tagtggcgga ggcgtgtgat gacgaagacc agccctatcg gctgtgcgag
     3301 ggctgccagc accggctggt tcatcgctcg ttgcggccga tggagtggta tcggctcgcg
     3361 ctgcggcact gtccgcacaa cttctcgctc cacgatgatt tctacctcga tgacggcacg
     3421 gcggatcagc ccaaggaaga cgtggtggat gcccatttgt tacccgcgcc gagtttagcc
     3481 gacaaagcgg gcagtttgaa ggacctgtgg gagttcacgc tgacccgcta tgcattgagg
     3541 gaagagcttc ttgaggcttg gaggaagttt ccggcatctg acatgcttga atggctgacc
     3601 acggcctacg attcgaggga ggattgccat tgtcggtcca cggttctcga actggcggag
     3661 aagtgtctcg ggcttgcggg tgctgatctc gtgcgccggg cgtggctcga ttatccggac
     3721 aagatgcact tcgcgggttt ggcaaatgca tccgttgcct gcttgcctga ggaggatgga
     3781 tttgagagat gctttgctgt gttcgacgcc cttccactcg aggagcaacg ttcacgcatg
     3841 atgtacatgc ggaaattcag aaatccccag atgctcgatt ggatcgaaac acacattcaa
     3901 tctccagtaa ccgatgtttt cagagctggt ggacttcttc aaattgcacg gccattgcaa
     3961 cgtgcctgag gactggaccg cgcagcccga acttgcgcgc tgggtcacga agcagcgccg
     4021 ggcgaagaag cagaatctcc tcacgctgga gcaggtgagc cggatggaag acattggctt
     4081 cgcctggagc acgcacgacg gcggctggga tgccatgttc gcaaaattgg tcgagcacat
     4141 gcggcccatg cacaacggca agccgcgcga aacacctccc agcgacgagc tcaaaggatg
     4201 ggtgctgaca cagcgccagt tcaagaaacg tggcgacctc gacccagaac gtgagaagcg
     4261 gctcgccagc atcggatttg agtgggagcc gttctctgcg caatgggagg cgatgtttgc
     4321 acggctgcgc gcctaccatg cagaacgcgg caattgcctc gtgccctcga agtggcccaa
     4381 agaccaacaa ctcgcgagtt gggtcggcac acaacggatg cggaagtcgg ggggggcaag
     4441 ctgtcagcag aacgaatctc gaagctcgac acgctaaact ttgaatggcg gccaggccac
     4501 agcggcggcg gcgaggtgga acgtgaacct tgggagaaga tgttcgcgca actagaggcg
     4561 tttcaccacg ccaccggaca cgcatcggtg cctcaaacat acgccgccaa caagaagctt
     4621 gcgtggtggg ttaccactca acgccggaag tatcggaagc agaagttagc cgagtggcaa
     4681 atcgcgcgcc ttagcgaact gaattttgaa tgggacggcg ggcgaaaggg cggcaggccg
     4741 agaacgaaca cgccgaagcc gccgcgtggt tataagccgc tctccggcga gaaaacttcg
     4801 agacgatgtt tcaggcgttg ctggaataca aacagtcgcg cggtgactgc ctcgttccgc
     4861 agcggtggaa agaaaaccgc aaactcgcag agtgggttgc agaacagcgc atggcctaca
     4921 gccgcgaacg tctcgcccca gatcgcgtgc ggcgactcga tgaggtggtc tttgagtggg
     4981 atcctatcgg cacacgctgg aaagagatgt ttcaacaact cgtcgaattc aagaaggagc
     5041 atggccacac gaatgtcccg cagcgctcgc acagatacgc cgagcttgcg acttgggtgc
     5101 ggaaccagcg agcagcgaag gcccacaacc gcctcatcat cgccgagcga ggcaggaggc
     5161 tcgacgaaat cggcttcgtc tggcgtctgg ttgagcgaaa cgcatgggag cgaatgttcg
     5221 accggttggt tgaattcaag aaactccatg gtcactgcaa cgtcccgcaa aaaggcggag
     5281 gcgataagct cctaggcaag tgggtgaata cacaacgaac gcacttcaag cgaggaacac
     5341 tgaagcccga acgagttcaa caactcaaag aagttggctt tgtctggaac acgaaggtgc
     5401 ctcgtggtga atcacggtag ggccacggcc tcatgcaggc catcgcccgc gtgaaccgag
     5461 tcttccgcga caggcccggc ggtctggtgg tggattacct cggcctcgtc gatcaactca
     5521 aacacgccct cgccaactac accgagagcg gcggcaaagg cgatccgacc tatgacacca
     5581 agcaggccat cgccgtgatg ctggagaagc acggcatcgc ctgtgacatg ctgcacggct
     5641 cggattggca gaaggcggtg ggcgacggca agaagacgct ctttgccctg cctgcgctgc
     5701 aagagtacct cctcgaactt gaagacggca agacgcgctg gaatcaggtc atcgagggaa
     5761 atgacgaatg aggagccgag cgaagcgaga cagacgactg aaaggagacc gaaggggagc
     5821 gcaacgaatc aatttcgaat gtcgaactgg cggaacttcg ccattcgcac atcggacttc
     5881 gcacttcccc tgacgccgcc gtgcgcctgc tcgtgagcaa ggcgatcacg accgacgggc
     5941 aggtgattga tgtgttcacc gccgccggat gtgagcgcca gcggcgcggc acatgccagc
     6001 ccagggcaac gccctgggga tgcgtccccg tttgatcaag ccttgaaggg gcggcacaac
     6061 cgttgtctcg ccctttcagg gcttgggatg gttgtgacca tggaacccag ggtgttgccc
     6121 tgggctggca tatcgcgccc cttcggggct gaacgcgcca tcagcacgat ggaggtgatc
     6181 gaggaactca tccgcctcgc caaggagatg gatgccgcgg ccaagcgcgg cgaggacatg
     6241 ggcctgaccg atgacgagat cgcgttgact ccttcgctcc cgctcagtcg ggctgcgccc
     6301 atcgcttcgc gctgtctgtc tcgctcgcct cccggcggct cggctctacg acgccctcgc
     6361 cgccaacgaa tccgccgtga aggtcatggg cgacgacaaa ctcaaagtca tcgccgccga
     6421 actcatcacc caggtgcgca agagcgcgag cattgactgg acgcttcgcg aaagtgcgag
     6481 agcgaagatt ggctccctcg ctgccgccgc tcggtcggcc tgcggcctgt gatttgcaca
     6541 gcctgcttgc ctcacttcgt tcgccctgcg ggcagccttg agctggctat ctccggcttc
     6601 gcctgcggtt cagtcggccc gctccttcgg ctcgcgtgat ggtgaagcgc atcctgaaca
     6661 agtatggcta tccgccggac ctccaggagg aggcggtgaa gaccgtgctc gcccaggcgg
     6721 agctgctgtg cgcggagtgg gtgtgactgg gaatgaccga caagttcctg actgaaaatt
     6781 caaggcaacg gcctcgcccc caagtccgcc gccgacttcg ccttcctgct gcacggcttc
     6841 cacttcctca aggacgaagg cgtgatgggt gagacttgga gacgaggaga cttggagccg
     6901 tgttgtccca agcgttcggc attgcgagaa ccaaatcggc agggttttcc gggccaaggc
     6961 caaaacatgc gctggaacat gaaggaaccg atgcatggct caagggcggc atggagcaga
     7021 tgccgaatgc ctgccggcac ttcgcccggg cattcgtcaa tcgtctccgc ccaccctgcc
     7081 ccggttgcgc ttcaccccgg aacggtgccg cttgccctca agcatctccc gcttcaccga
     7141 gcggggccgc gaccggtttt gacggcgttt tttctcccgc tcctcctgga tggcgagccg
     7201 agcctgctcc aatgcggcct ccatgcgctc gcagagttcg acccgcgccc agtgccggtt
     7261 catgacctgg gacctctcgc gctggcagcg cacctcgata ccgctgggga cgtgcctcaa
     7321 gacgacggtg gaggaggttt tgttgatctt ctggcctcca ggaccgccgc cacggatgaa
     7381 gctctcctcc aagtcctcct cgcgaaggcg cagcttgcgc atccgtttca gcagggcgtc
     7441 gtcgtcgggc agtggcatga cgcgagaata gtgccgggcc gccgtgaggc aagcgcgccg
     7501 gccgcggcgg cgggagtttt tgacacaaac gccgcggccg cgcgttccca tgccccgatg
     7561 aaaacgctga tccttccttt cttgtgccta gccgcgcttt tccacgccac cgcggccggc
     7621 aggccgaaca tcatttttgt catggctgac gacatgggct gggggcagac gggctatcgc
     7681 gggcatccgc tgctgaagac tccgaacctt gacgccatgg cggcgaacgg gctgcgcttt
     7741 gagcgcttct acgcgggcaa tcccgtgtgc tcgccaaccc gcgcctcggt gctcaccggg
     7801 cggagcaatg accgggccgg cgtgctcagc cacggttacg cgctgcggct ccaggagaag
     7861 accatcgccc aggcgctgaa gtccgccggc tatgccaccg cccactttgg caaatggcat
     7921 ctcaacggct acaagggacc cggcgcgccg gttttgaagg acgatccgcg cagccccggc
     7981 gcgttcgggt ttgatgagtg ggtgtcggcc accaacttca ttgacatgga cccctggctg
     8041 gggcgcatgg gagcgccgga gcagatcacc ggtgattcat cggaagtcat cgtggcggag
     8101 gcgtctaagt tcatcgagcg gaaaaaggac ggcccgtttt tcgccatcat ctggtatggc
     8161 accccgcaca gtccgttccg cgcccttgag gctgacaagg cgccttttgc ccacctggac
     8221 gaggcgtcgg caaatcatca tggggagctg gtggccatgg accgcagcat cggggcgctg
     8281 cggaagaagc tgcgggacct cggcatcgcg gacaacacat tgctggtctt caacagcgac
     8341 aacggcggac tggccggcat ccagcccgga accaccggcg gcctgcgcgg caacaagggc
     8401 acggtttatg agggcggcct gcgcgtgccg gggatcatgg aatggcccgc cgtcatcaag
     8461 cccggacggg tgacctcctt ccccgcgagc acgatggacc tcttccccac cgtggcggac
     8521 atcgccgggc tgccggagac atgcttcatc cagccggtgg acggggccag cctcaaaccc
     8581 ctgctcgccg gcacggaaag cgggccgcgc gtgaagccca tccccttccg ctacgggcgc
     8641 aaggcggcgc tggtggacaa catgcacaaa atcctcaccg acaatctcga cggcggcgcg
     8701 tttgcccttt acgacctgga gtcggacccg cacgagacac gcgacctgag cgccgacatg
     8761 ccggagttgt ttgaaaaaat gaaaaaagac ctgctggcat ggagcgccgg cgtggatgcc
     8821 agcttcgcgg gcaaggacta tccggagggc gcggtctccc cggccgatcc cgtgccccag
     8881 aactggcatg aggcggagcc gtatctcaaa ctggccggcc tgtggaaagg ctactgggcc
     8941 tacgactccc atatcaaccg ccaggccaaa ggcgccgcca aaccaggagc aaagaaaaag
     9001 aggcaaccct gacgcgccgg tgcattccgc gtcaggctca acgctggtcg tcgagtccgt
     9061 tggtcacctt gccttgcttc aaacggttgc gcaatttcga ccagaagtcc tcgtcgtcgg
     9121 cggaagggcc gctttcgggc aggatttttg aagcgcctcc aggaagggtc ggcacctgga
     9181 tgacggcccc gtcatcaggg cgcatgtccg cctgcgagcg gccataaggc gcgaagtagc
     9241 caagagggat cggatacttc tgaaagctgg tcggcagggt gcggtgcttg ttgatgaaat
     9301 ccgtcgggct gtagtagttg ctgttgtccc gggcgaactg ggagcggttc atgccgatgc
     9361 ggaggttttt gcgcatctcg aagtggagat gcacataata catgccgttg ttcccgccca
     9421 tggtgccgac gagctggccc ttctccacca cctgccccac tttcacgccg cgttgatgca
     9481 ggtgggcgta gagactgtcc accatggcga tcttgcccgt ggcatcgcgg taggcatgac
     9541 gcacaataac gcagttcccc cagccggcgc gcacatcctc ggaaatgacg accacgcccc
     9601 gcgcgcagga gtaaatggga tggccgagat cgttgttgcc gccgctgcgg tggttccaat
     9661 cctcgccaag atggccgttg ggccagtagc cccgcgattt gtaatagccg tccgcgttcg
     9721 gcttgcccac ggggaaatca aacccatccg caagctggca gcgcacctgg ccggaggact
     9781 gcgccgagac ttgctggaaa cccgacaagg caagagcggc ggcggcaaag aggctgcgcc
     9841 gggcgatggg tgctgccttg ccgccggaca tgggaatcaa tatggtataa ccgcgcggcc
     9901 tcagttggac ttgggagcgg caggctccgc ggcgggtttg tcagcggctg ctggaggagc
     9961 gggaggcgct ggctcggcgg gctctttcat cgctggagtt gcggctttgg gagcgggttc
    10021 ggcagcctgg ggagtggcgg gagcgttttt cgcctccgca ggcggatttg gcgcgccggc
    10081 aggcggagcc gggggattcg cctcgtcctt tttggctggc gcggtttcgt ccttggccgg
    10141 ggcagcggac ttttcaccgg ctttcggggc ggcatcgcct gcggcagccg gagccgggct
    10201 ttccacaggc ttgggagcct cgaccggcgc gggaggcggc ggctcatcga agattttggc
    10261 atttgccctg gctgtctgct ggcgggcggt gagcatggaa aggccgagag tgagaacgaa
    10321 aaggatgatg gccagataaa ccgtgaacct ctgcagcacg ttcgtcgtct gcgcgccggc
    10381 gatctgcgac atcatgtcac cgccgaacga ggctcccagc ccctcctggc gggggcgctg
    10441 catgagaacg atgaggatga gaaggaggca gacgagaact tcgaccacca tgagaatgcc
    10501 gataacaatg tccataaggg gcgctcacta tgagggcact tccccctttg gcaagctgct
    10561 tctggacgtg cgtgaaagac acgcgcccgc cgcccgcgcg aggcgggtct atttgccttt
    10621 cacacgcacg ttttccgatt gttcgacggg cgcctccagg ggtttgtcag gcattttcag
    10681 ccaaggcatc agcatggagc cgagcagata aatcaagccc gcgccaagtc cggcgccgat
    10741 ggcgatgaag cgccgctgcc agacattctc gtggctgatc agcgtgtaga agcgcagaca
    10801 gagcagcacc cagaggatgc acaggacggg gatggcggca ttcaagagca tggcggcggc
    10861 attgaatcaa aaacaccgcg ctttgaccaa cctgaatttt gaaattcacc cttcccatgc
    10921 aggctcgata aaactcccaa gctgaaactt gaaacctcga agcccagcgc cgatggctgc
    10981 gcgccgaaaa attcgagctt cgagtttcgg atttcccacc gcatgagcac ctcacaaatg
    11041 tcccgcccca gcctctccag gcggcctgag tcgcaggcgc gggaacctgc gggcctcatg
    11101 cggaggctct ccccgctttg gtggtggcgg cgctggttcg ccgaggaaga cggtgcgccc
    11161 gtgctcgcct cgcgcgacgg caattccatg ctgcccgtgc tcatcgaggt cttcgccgga
    11221 ttttcgaaac tggatggcgc ggtgcaggag gatgagatcg agtcctcgct cggctacctg
    11281 cgctacgatt acagcggcac gctctacgcc gacctgcgcc ggctttattt cgaggcgctc
    11341 caggagccgc aggacctgtc caagcgcgcc agcgaactcg ccgaggagct gaccctggag
    11401 cagaaaatcc tgctctgcgt ccagctctac ctgctcatct cccgctcgct gaccgaccag
    11461 cgcatgatgg tcgagtttta tctcttcatg acgaacctgg gcatcgccgc ccaggccata
    11521 gacatcgtct atcagctcaa ctcgggggac aaaccccagg acgaggagtt cagcggcaag
    11581 accggccagc ctctggaaac cctgcgcatc ggctccggcg ggggctgcga tgtgctgctg
    11641 cgcacgcttt ccaccgactg ctcggtgatg gcgttccgct atcaaaacct cttgctgctg
    11701 aaaaacaccg gagccattcc cgtgcttgtg cgcagccgcc gcctgccacc cggcgagttt
    11761 tcccgcctct accctgccga gcgtgtggtg ctggaggacg tgacgctcga ctacaacgac
    11821 ctcgcctcct acttcagcgc caaaaagacc gtcaccggcg cgcagctcta cctcactctg
    11881 accgaaacag gtgatgccga gatcgccccg cagcgcgcgc gcggcacgaa cctgcgcgtc
    11941 agcttcggcc tcggcgtcag catcgaggcc ctgcgcaaca ccgacgccac cctcaacggc
    12001 gtgctcttga aggaaggcac ccgcgtcgag gcatccatcg aggacagcat catcgcccac
    12061 ggcgagatcg aaatctccct gcgcgacctg cgcgtccgcg cccaggagtt cggcggccag
    12121 ttccggctcg aaggcagccg gaacacctac ctcgtctcca acaaccccga gctgctcgac
    12181 gagggcgaca ttctgctctc cgacgacacc gagggcgaga tcctcctgcg catcgagtgc
    12241 aactacgcgc agaaaaccgg caccctcgat ctgctgcgcg cctcccgccc catctacgtc
    12301 ggccaggtgc ccgtgcgcga ccgcatctcc ctcagcgacg gcgacaccat caccctcggc
    12361 gagggccagt tcctgcgctg ccacttcgcc gaccgcatca tcgaggagga aaaaaacacc
    12421 atccgccaga tggaggtgcg cgagctggcg caccgtttcg acagcaagga caccgcgctc
    12481 gacggcatca gcttcagcgc gcggcgcggc gagatgatct gcgtcatggg ccccagcggc
    12541 tgcggcaaaa gcactctcct gcgcgcgctc ggcggccagc tccgcacccg cggcggcgac
    12601 atcctgatga acggcctctc gctgtatcaa aacctgccga acctcacccc ctacatcgcc
    12661 tacatcccgc aggacgatgc cttcgacccc ctgctcaagg tgcaggaaaa cctggacttc
    12721 tccgtcgccg tacgctgccc gcacctgaaa accgaggagc gccgcaagcg cgtcgaagcc
    12781 aagctcgccg aactcggcct ggccgacatg cgcaaacggc tggccggcac cccgcagcag
    12841 aaattcctct ccggcggcga gcgcaagcgc ctcaacgccg gcctcgacat gatcggcatc
    12901 tccgacatct acctcttcga cgagcccacc tccggcctct cctcgaagga cagcgagcat
    12961 gtgctggaaa tcatccgcag cctcgcccgc aacaagctcg tgctctgctc catccaccag
    13021 ccgagcatga agctcctgca aatgttcgac aaggccctcc tgctcgacaa gggcggcaag
    13081 atggccttct tcggcacccc gcaggccatg ctcgaatact tctggcgcgc ctacaatgac
    13141 gagaccggcc agcccggcgc cgccatgccc gccaacctca cgcccgactt cgtcttcgac
    13201 gtgctcgaga cacccctgcg cgacatcagc ggagacatca tccaggagcg aagcgccgac
    13261 ggccacctcg tcgccgcgcg ccgtttcccg cccaatttct ggcgcgaccg ttatcaaagc
    13321 cacctgctgc tcgaaagcat ggccaaagcc caggccgcgc ccggcagcac caacctcatc
    13381 gcccgcccgc gcgaggacac ccagagcggc tcgcgcggcc gcgccatgcc caagcctccg
    13441 aagcacaccg tgcgggagga gagcatcctc ttttacacca tgctgaagcg cgccttcctc
    13501 tcgaagctgc gcaaccgcac caacctgctc accaccctgc tggaggcccc cctgctggcc
    13561 ttcctcatct ccagcgtcct caaatacagc gaggaggacg cctacacctt cgccaccgcc
    13621 tttcacatcc ccacctacct cttcatgagc ctcgtggtgg ccatgttcct cggcctcacc
    13681 aacagcgccg acgagatcat ccgcgaccgc catctgctca gccgcgaacg caaccacaac
    13741 ctgcgcactt tttattacct cctgggcaag atcctctccc tgagcgcctt cgcccttgtg
    13801 cagtgtgtga tctatctttt catcggcaac tgggtgctgg agatccgcga catgtttttc
    13861 acccatctct ggtggatgtt tgtcaccgcg ctgactggcg cctgccttgg actgctcatc
    13921 tccagcttcg tgggcgacag ccgcaccgcc atcaatgcca taccgcttct gctcattccg
    13981 caaatcatcc tcggtggcgc cctcatcaaa tacgaggaaa tgaaccgcaa cctggatctc
    14041 atctactccc tccagccctg gctggtgaag gccggcattt atgaggagga gaccaagacc
    14101 aagctcaagg tgcccttcat ctgccagttc atgcccctgc gctggagcta cgagggcatg
    14161 ctcatctccc aggccaagct caaccctctg acacacgcgc aggacacgct ggaggagcgc
    14221 atccaggagc tcgtcagcat cccgcccgag atcaagctca ccaacgacca gcaaaaagag
    14281 ctcgacgccg ccaaacaagg cctcgccgtc gtctccggcc tgtatgccaa ggacagcgat
    14341 gccgccgcga aaatgctcgc ccgcatctgg gatgccacgc tgcgcggccg cctcaccccc
    14401 gagcttttga aggaggcgct ggagcagcag gacggcacca gcgccgagga gatctatgtg
    14461 aaccgcaaag tgctcgacct cgtcaccaaa gccgagatgg agcgcaccga ctaccggcgc
    14521 aaagccagcc ccaacgtctt cttcggcacc tccaaaacct actttggcac gcccttcaag
    14581 accctgtgga tcaacagcat ggtcatcctc atcacgcttg gcctcatcat cacctgcgtc
    14641 gagttctccc tgcgccggca gctcacgcgt gtgtgaggaa ttttcagccc gaaaaaccgc
    14701 ggacctcaag gcttgcgctt cacgagccct cccccgcttt gcggcacctt gagcccggga
    14761 aagtgatcgc cctcgccaga ctcacccggg cctgttttga agaatatccg cgcgatggcc
    14821 gcctgggatg agtgagcggc ttcgcatggg ttttgacgca ggtcggtgag cttcatgggg
    14881 tgcaggatgg attcccaatg agttcgctga ccaagcgctt cagttcatca ggcgtcagaa
    14941 ccatggtttt tccggccagg cgcagcacct cgggtgctga tgccagccat tttcagcctg
    15001 tgatgacacc tgacttggcg ggactttcgc cgcagtcgcc ggcttgtttc attcccgcgg
    15061 ctcacccggc tgctcacttc acgcgcgcgc aacatgccac tggcgctcca gacagggtgg
    15121 catgggatga ctgtcacgcc atgagcatgg aaggcggcag cctgcggctg gcaggctgcc
    15181 gcgcgggacg gggtcagaat ttcagcctga tgcccgcata agcgccggtg tcgaaggcgg
    15241 ggaagccggc ggcgtcctcg tagttgtcgc cgaggagatt ttcgacgcgg gcgaagacct
    15301 cgaagttccg gcaaacgcgc cagttcactg tcagtcgggc cagcaggtag tcctccaggt
    15361 ccttttgcgc gaaggtggcc gggtcaaagt cctcgcggtc catgatatag agggcgctga
    15421 gattgacggt gactttgtca tgcggccgca ggctgactcc gccggagagg ttgtgacgcg
    15481 ggcggcgcac gagccgcgcg ccggcgccga ggtcgtcggc gtcgaggtag gtgtaggcgg
    15541 cattgaagga gagaatttca tgcggctccc acaagacgcc aagctcgacg ccctgggtgc
    15601 gggcgcgccc gatgggctgg agggagaagg tgccgagatc gaattgatag gtgtccttca
    15661 actcattgcg gaaccaggtg gcgctgaggg tggtctttgc cgccgggatg ggctgctcga
    15721 tgccgatctc ccatccgcgg ctgcgctcgg gcctggcgag ctgcggattg ccaaagagcg
    15781 cgggctcgcg gtcctggccg ctgggcgggg agaaggcggt gccgtagttg ccgtgaagca
    15841 gggtttgcag cacgggcacg cgccatgcga gtccggcgcg ccaggtgacg gcgtcgtcga
    15901 agtcggagta gcggtcgtag cgcaggccgg agatgaagtc gagaccgggc aggacttcga
    15961 gctgggactg gaggaaccat gcccagttgg tctcgttttg gtcaatgtcg aggctgcgga
    16021 tgttgtcgtt gaagcgggtg aggttttgat cctccagcca caagccggtg gtgatggtcc
    16081 aaatgtcgaa tgggcggaac tcgctttgat actccaggaa atggtggcgg atgctgacgc
    16141 ggttgttcgg ggtgaagaag cccgtgaagc ccgaggcgac ctggcggaag ttgttgaagg
    16201 aatacgtcag gctctgccgc cacccgtcgg tggtgtccca gatgacgcgt ggtgagatac
    16261 tccagaattc ggtgagcagg ttgtcatcac gatcattggc aaagcggctg ccgggaaggc
    16321 cgacctccgc ctcataccag cggccgtcga gttcaaagcg cagggtttcg gcgatctgcc
    16381 agccgatgcg cccgttggcg ctggtctgcc cgtagcggct gttgacgcgc tggccctgga
    16441 catccgcgcg cgccgcctca aaggaccagt ccagatcacc cgccgcgcta agggtgctga
    16501 tcccctcgcg gaaggtgccg taactgccgg tctcgaagaa gacgctggtc tccggcttct
    16561 ccagtccgcg cccgctgcgc gtgatgatgt tgatgacgcc accgatggtc ttgccgccgt
    16621 agatgcctgc ggcgggtccg cgcaggacct cgatccgctc gatgttgtcg agcggcgtgg
    16681 tttccaggtt gtaggcgccg gcgaggtttg cgggcacagg ccgcccgtcc atgaggacgc
    16741 gcgtgtgctc ggtcttggtg ccccggatga agacgccggt gacggtgccg ggcgcgccgc
    16801 ggtcggccag cgtgagaccg ggcacctcgc gcaatgcgtc caccaactgg cggtattgct
    16861 tttcctcgat tttggcccgg tcaataaccg tgatgcttga ggctgtcctg gccgcctcgg
    16921 tctcggtctt ggtggctgtg accaccaccg gcggcagcgg cttgtttgtc cccgcttcgg
    16981 cgggggtttc cgcgccatgc ccgaggcagg cgcaggtcca tgcgatgccg gcggcgccga
    17041 gggcgcgcag gctcttctga gtctgtgtca tgtgtttgtc ttgcgatgct gcggcaagag
    17101 cgggctgcca tgaggccggc ggcttttggc aaagcgccgc gcatggacac ccgttcccac
    17161 ccttcattgt tgcgatgaag ttttgacccg ccggcttcca ctcggaaggg cgggtgtgag
    17221 cattcgcagc gagatattcg gactccgggt ttgtggcggg cgggggctgg agactccgga
    17281 ttgaaattcc cagactgccc cggccgcgcg cctttttcgc tcgccttcac cggtgagaaa
    17341 ctctccgatt ggcttcaccc gcacgctgtc accagcgcgc gcgtcgcgaa attgtgaccc
    17401 gataccgcag cgccactgtg gccggttctc acggccttcc ctgcatctgc gaacggctgc
    17461 cggcggatca ttgcctccgc cgggcttgga aagaaaacac cctcctttgc gggagggagc
    17521 cctgaccgcc gatagccgcc gtctgaacgg cgcgcaagct gaaatgggat cttgtcacaa
    17581 gccggcggaa cgcgcctcgc cgacgaactc ctccatgagg tccatcgctt tcacaatgag
    17641 atcgggcgcg gtggcgtagc agcagcgcac gaaaccctct cccgccgcgc caaaggcgct
    17701 gcccggcacg acggccacgc tttttttctc gagcagcctg atggcgaagt ccttgctgcc
    17761 cagccctgtc gggcggatgt cgggaaagac ataaaacgcg ccgccgggat tgaagcagtg
    17821 caggcccatg ccgttgagac gggagacgat caagttccgc cgctgctcgt agctctggcg
    17881 catttcctca taggcgttgg cgcccatctt cagcgcctca atgccggcca tctggctcat
    17941 gatgggcgcg caaagcatgc agtattggtg gattttcatc atcgcctcgc gcagcggcgc
    18001 gggagcgcag gcgtagccga ggcgccagcc ggtcatggca aaggctttgc tgaagccgtg
    18061 cagcaggatg gtgcgctcct tcatgccggg gaattcgacg atgctggtgt gcttgcgccc
    18121 gtcgtagagc agctcgcaat agatctcgtc ggtgatgaca atgaggtcgt gcttgatggc
    18181 gagccgcgcg attttttcca gctcctccgg gggcatgatg ccgccggtgg gattggtggg
    18241 gaagttcagc gccagcacgc ggctgcgcgg tgtgatgacg cgctcgacgt cctcagccat
    18301 gagggcgaac ttgttttcct cccttgtctc gaccacgacg ggcacgccat acgccatttt
    18361 gatgccgggg ctgtaggaaa cgtagcaggg ctcgtggtag atgacctcgt cgccggggtt
    18421 gagcagggcg cggaaggcga ggtcgagcgc ctcggacaca ccgacggtga cgaggatttc
    18481 cgtcttcggg tcgtaagtga cgcggaattg cttctccaca taatcactga tggcgacgcg
    18541 cagggactcc aggccgagat tcgaggtgta ggaggtgacg cctttttcca gcgagcggat
    18601 ggcggcctcg cggatgggcc agggggtggg cttgtcaggc tcgccgacgc cgagggaaat
    18661 gacatcggag cggccgatga cgagttcaaa gaagtcccgg atgccggagc gtggaagatc
    18721 gcgtatctgg gtgcagagct tgctgtcgta gtccatgggt cgcggatcaa atcatggact
    18781 gacgggcagg cgctcctcct cgttggcgga gttgaccagg aaaccggact gtttgtaggc
    18841 cttgagctgg aagtgcgtgg cggtggagag cacgccgccg agggtgctga gcttttcagc
    18901 gacgaagttg gccacatcga gcaggtcctt gccctcgacg atgacggcga ggtcgtaacc
    18961 gccactcatg aggaagcact cgcgcacctg gtcaaacttg gcgatgcggc gcgccagacg
    19021 gtcaaagccg ccctcgcgct ccggggtgat gcgcacctcg atcagcgcca tgacgccgcg
    19081 atgccccgcc ttgtgccggt ccacgacggc gttgtatccg agaatggtgc catcggcctc
    19141 ggcggcctgc atccgggtga tgacttcggc ctcggtgagg cccaggattt gggccagctc
    19201 gtgggcggag cggttggagt tgatctggag gagttgaatc agcggatcca tgaggacgcg
    19261 gagactaagc gataccgcgc tggatgcacg tttttgttga gcctgatttg aggcgcgttt
    19321 gcggtgatca atacctcagc aactcgcgca atgcattgct gatgctgccg gtggtcgggc
    19381 ggtgggactt ccacaaattc gggtgatacg ggatgggcgt gtccttggag ttcaagcgca
    19441 tgggcggcgc gtcgagcatg tggaaggcct cgctggtgac gcgggcgatg acctcggcgg
    19501 tgacgccgcc ccagggaaag gtctcgccga cagcaagcag gcggcctgtg cgcgagacgg
    19561 aggccaggat ggtgtcggtg tccatgggtt tgacactgcg caggtccacg acttcgatct
    19621 ggtagccgtc gcgctgaagc tcctcggcgg cgcgcaggga ttcgtggagc atggcgctgt
    19681 aggtgaccac ggtggcatca cgacccgggc gggcgatgcg cgccttgccg atgggcaccc
    19741 tttgtccgaa gtcctccgcc ttcaggtgat agtagaggaa cttgtgctcg caaaaaatga
    19801 cggggtcgtc cagctccacg ctgtccagca gcatccagta ggcatcctcc acggttgccg
    19861 gcgtgaggat gacaaggcct ggatagtggg cgtaaatgct ctccatgctc tggctgtgaa
    19921 aagggccgct gccggcggtg ccgccgctgg gcagccggat ggtgatgggg cacggtgtct
    19981 gcgtgcgcca gtagagcgtg gcggcctggt tgacgatctg gttgaagccc accgacgaga
    20041 aatcggcgaa ctgcatttcc aggatcgggc gcgcgccctc gatggccgcg ccgacagcca
    20101 tgccgaccat cgcatcctcg ctgatgggcg cgtcaaagac gcgtcccggg aactcgcggc
    20161 tgagattctt cgtggccttg aaggcgccgc caaaggtgct gatgtcctgg ccgtagagaa
    20221 agacatccgg gttcgtgcgg agtgcctcgt attgcgcctc acggatggcc tcaaggtagg
    20281 tgatgctcat ggattctgcc cctccacaag cgaggctgtg gccagcgcct gccaggtttc
    20341 gcgataagga tcgggtgcgg gttcccgccc cgcttgcgcc tgggcggcgt ccacctcgcg
    20401 ccgcgcctct tcctcccatt cgcggaagat tttttcatcc gcccagcctt cgtccagcgc
    20461 gcgctgcctt gcgaccacca gacagtcgcg cccctcatgc tggtgtttca ccacatcggg
    20521 gatgtaggag gcgtcgtcat gctcgccgtg gccgccgagg cgcagaagtg cgccaaccac
    20581 caactgcgga ccgtgcccgc cgcgcgctct tgcaaaggcg gtgcggaaaa cggccagcgt
    20641 ctggtaaaga tcggtgccgt ccacactgta accctccacc ccatacccgg cggcgcggtc
    20701 cgccaaatgc tcgcaggcgt actggcgcgc ggtgggcgtg gagtaggcga actggttgtt
    20761 ggccacgctg acaatgagcg gcagtttttc cacggcggcg aggttcagcc cctcatgaaa
    20821 cgcgcccgtc gaggtgccgc cgtcaccgat gctggtggcg ccgacactgt cacccaggac
    20881 tcccttcaaa cgccgggcaa acaacgcgcc ggccaccacc gcgacgaggc tgccgaggtg
    20941 ggagatcatc gccatgtatc cttcgcgcgg tttgccgcgg tgaatgttgc catcccgccc
    21001 gcgcatgggg ccggtgacag tgcccagata ggtgcgcagg gtgtcgagca caggctcgcc
    21061 ataagcgatg cgccccgcct ggtcgcggat gagcgggccg taaatgtcgc tgcctttgcg
    21121 caggtggatg ccggctgatg cgctgaatgc ctcctggccg cgtccgaggt aaactccgcc
    21181 gacgatccgc ccgcccgcgc ggtagaggct gccgagacgc tcctccaggg ttctggagag
    21241 gatcatgcac cgcagggcgc gcagaaaatc atcgcgcaaa tccacgggca tcaaagtctc
    21301 ggatggggag ggagcggcgg gcacggagga cgatgtcgtt tcagccattt ctcacggcac
    21361 ttgccggatg agatcggcgg attgacggag attttttcac acggcttgaa acttgtccag
    21421 acaggaaatg cgcggcgctc aggcccacaa gttctccggg tagcgccccg ccgccacgag
    21481 cgcgcggatg ctggccacca catcggcctt gtcctcgggg taggtcacgc cgaaccacga
    21541 atcgttcgac tccagcacgc gcacgtccgc cttgccggcc tggatgaggc tgtccaccac
    21601 cttgggcaca tagcattccg ccttgagatc acccgcattg gcattcagaa actccacaaa
    21661 agccgcgcgc agatgcccga agatgtccgg ggtgaatccg aagaaattca tcgagaccac
    21721 ctccccgccc gtcagcggca gaggcggctc cgtctcgcga ttctccgcgc cggacggtgt
    21781 cttgaagatt ttcgtcatct ctgtcaccga ggccagcatg ccatccgcgc cgcgcgtgca
    21841 gacgccgcgc gccacgctgc cgtgctccga gagcgtgttt ttgagataaa agcccaccat
    21901 ggagtaatga ctgcgcccgt cctgcgcgcg gggtgccgtc aaatccgcgg cgattttggc
    21961 gaaggcatcc tggccgtaga agtcgtccgc attgatcatc aaaaacggct cccgcaccac
    22021 ggactcggcc gccagcaccg cgtgggcggt gccccagggt ttcgtgcggc cttccggcag
    22081 tgtgaagccc tcaggcaggt cgttgaggtc ttgaaaggcg tactccacct cgatgcgccc
    22141 gccatatttg ccgccgatct gcgcgcggaa ctgatcctcg aaatcccgcc ggatgacaaa
    22201 caccaccttg ccaaacccgg cgcggatggc gtcgaagacg gagtaatcaa ggaccacctc
    22261 gccggagggg cccatggcgt cgagttgttt gaggccgccg tagcggctgc ccatgccggc
    22321 ggcaagaatg aggagggtgg gtttcatggt cgggggatga aggccgcatc tatggatgtg
    22381 ccgcgccggg cgtcaactga gagaaaccgg cgcggcactt cggcgggcga ttgtcctgaa
    22441 aataagatga aacgcgcagg ctctggaggc agtctgtaag cccatgcaga acggcctcaa
    22501 aatcctcatc gtcctcagcg cgctcgtctg cgccgccgct ctggtcatgc tgggcgggct
    22561 ggcattggag accaccgcgc ctgaccgccc ggtgaaaatc agcaaaccgc tcaccaactg
    22621 gggggcggga gaccggcgcg gcagcggtcc cgatttcagc ctgcccccgc ctgatgccgc
    22681 cgtggatgat ctcgccgaat tgatgcgcaa gctgctggaa ctctcccgct cgccgggcgt
    22741 ggagcccgac gagctgctgc tcagcttcaa atcccgcgcg gctctcgacg ccttccgcgc
    22801 ccgtgccgcg cgggacggga tcgaggtgct ttactcggat ccacgcctgc tcaccgcgcg
    22861 tgtgcgctac ggcgacaccg cggcaatggc gcgcgaactg cgcgacaacg cgggagccta
    22921 cgacaacatc ggcatgaatt tccgcgcctg ggtgccggcg ccccagcgcg tgccggaaac
    22981 cgacaccgca aacgccggcg gcatgaggcc ctatggcgac agcggactcg atgccatcgg
    23041 cgcggcgggt gatcgcgcca cctggggcaa gggcgtcacc gtcgccgtgc tcgacaccgg
    23101 catcacccct catcccgtcc tggagcactc ctccatctcg cgcaccgatt tcgtccagga
    23161 tggcaaggaa ctcgacgggc acggcaccgc catggcatcc ctcatcgccg gagaggatcc
    23221 gcaaaacggc ggcgtggcgc ccgcctcgcg cctcctcgac atccgcgttg ccaatggcaa
    23281 cggcgaaagc aacaccgccc tcgtcgccca gggcatcctg gccgccgtgg accagggcgc
    23341 gcgcgtcatc aacatcagcc tcggcaccaa tgccgactcg ctcacgctgc ggcgcgctgt
    23401 ggagtatgcc atcgcccagg gcgtgatcgt cgtggccgcc gcgggcaatg accagtccaa
    23461 ggcgctcgcc tatcccgcgg gctacaccgg cgtcgtcagc gtcgccgcgg tggatgccaa
    23521 tggcgtgcag gcgtttttct ccaattcggg tccggggctg ttcatctccg cgcccggagt
    23581 gggcatcgtc tccgcctaca gcggcgatgg catggtcatc agcagcggca cctcccaggc
    23641 caccgcgctg accagcgggg ctgtggccgc catgctcagc cggggttaca acacacagaa
    23701 tgtcatgcaa gccctggtca acaacgccag gccgaccggt ctgccagcca cgcagacggg
    23761 cgcgggcatt ttgcaggtgc cgcgactttg atcaacaaat caaccgccgg gtcatggcat
    23821 cgtcacccgc gcgcggagaa agcggatgac acccggccct gcgggcacgt tgtcgcgcac
    23881 gcgcagggtg gtggcgttgt ccacctccac cacgaggccg gcgctgctcc aggagagcgg
    23941 gtcggtcaca tccgaggtcg cttccacggt gtaggtgatg cctgtggcgt cggggttttt
    24001 cggcacggtc aggcgcagaa acttgtcctg gcccaccgtg gccgtgtcct gcgccagcgg
    24061 cgactcgtcc ggctccatgc cctggagacc gagggcgtat tcgagcaggt tgttccagcc
    24121 gtcgttgtcc gggtccatca gcaggccgtt cacggcgggg ttgttcgcct gcgcgccgaa
    24181 ctgggcgaag cgccatgcgt ccatcggacg gtctttcacc tggatcacgg agacaggttc
    24241 cacgggcaga tgataggtgg catccgcctg gaggttgatc gccagggttt tgtcaccctc
    24301 cgccacatca tccgccacgg gctgcacctg aatggccgcc gaggattccc ccgcagggat
    24361 ggtgacactg cctggcagtg cttgataatc ccccggcgcg ctggcgctgc cgcccgtggt
    24421 gtaatgcacg gtcagcggca tgtccaactg gccgttgcgt gtcaccgtca caaggcccgg
    24481 cgccgccccc tcctccgccg cgtcggccgc ggccccgaag ccgagtgtgg caaactgcac
    24541 gtccacagcc acatcgtcaa agctggacgc ctcgccgtca tccgcactga ggcgcagcac
    24601 ataagccccc tcagcggcaa actgcgccgt ggtctgcgcc gcggcggcat cctcaaatgt
    24661 caccacaccc ggcccgctga ccacggacca ttgcgcatcc ggcgcatccg gcgcgtcatc
    24721 cagcacgctg ccagccagca cgcggaaggc ggcggcgctg atggaaggcg tggtggcagc
    24781 ctgtgaaagc ggagccgcgt tttccggcgt gccgctgacc tggattcctg aaaagaggcc
    24841 gcgagcaggt cttgccgtgt tgccgctggt ggtggcaatg cccgcgcgca gggtggcggg
    24901 cagagcaatg gcctgcgcag tgcccacctg ggtccagtca atgccatcca gagaatgcca
    24961 ggcgctgaag ttgctgcccg cgcgcgccag cttcagccag cgcggaccgt gcggcaggct
    25021 cgcggtggtc gtgctcaccg ccccgcccgc cgtggtgcgc cgcgtccagg ccagggttga
    25081 ggtgatgttg ccgccggaga cagtgaccag caccgtcagg cccgcgcatc tcgcgttcgc
    25141 cgccgtgccg tcccgcagca tcacccctgt gcgcgcattg ctgaaaagac cgtccgtgcc
    25201 ctcgtagcgg gcgacgattt ccccgtcccc gctcaactcc gtgtgctgga aatgaaacga
    25261 gtccgagctg gcggtgatgc tggagcccgc gccctggttc tgcgcccagc cggagcggat
    25321 gtccgcgctg cccgtggcat tcgtgccggt gatgccgatg tcttcagcag cccaaagagc
    25381 gccgccccca cccaccgcca cagccacctc cgcatgagcg ctgaggctgc ccgcgtccgt
    25441 ggccgtgcag cgcaggatgt gccgcccggc ggcggaaaag accaccgtgg tttccagcgc
    25501 ggacgcctgg ccaaaggtgg ccgtgcccgg cccgctcaca tgggtccaca gcacgctggt
    25561 tgatcccggc gcgccgtcat ccgtgaccgc cgctcccaga tgcagcccgg tgctcaacga
    25621 caagcccgcc tcggcgcggc gcggctgggt gatgcgcacc gtgggcggat gattcgtcgc
    25681 gttgtcgagg atgtccaccg tggtgctggc ctctcccacc acgagatagc ggcttgaggc
    25741 gttgagcgtg gcgatgacgg tttccgttcc ctccgccaag gcatccggca gcggagtcac
    25801 cggcagttcc aggtgacgct caccggcggc aaagctggcc gtcgccggca tcgccgcata
    25861 gtccgcgccg gcggtcgcgg tgccgctcca ggccaggctg acctccagtg gcgcattcac
    25921 cgggcctgtg cgcgagaggg tgaaggcgcc gggcgcgcca ttcgactcat aggccgccgg
    25981 gtgcgccgcg cgcagggaca cgcgcggctc gttgtcgcgg atgaagagcg tgtggtagct
    26041 gcccccgctg gtcagttgcg cgccgtgggc gaatgacagc ttcagcgtga cgtgctcctc
    26101 cggctccggc agcaggtcat ccacgatgct caccggaatc tccacctgcg acactccggg
    26161 cgggaagacg aggctgccgg cttttagatt cacatccacg ccgctgcccg tggcgctgtt
    26221 ggtgtccagg atgtagtcca cgcgcacctc attgaccggg tccgggctgg aaagcaccac
    26281 cgggatcgtg aaaggcgcgt ccccctccgt cttcaccgtg cggcgagtgg caaaggaggc
    26341 cgtgcgggca aaacccgcgc tctcattgtc ggtgatgtgc agcgtggccg tggcgcgctc
    26401 gcggctgtaa cttccaggga ggatcgtcac aatgagagac tccgtgccct cggcatcgga
    26461 atcgtcaatc cctgtgatgt ccacgctcac attcgacgcg ccgctgggaa tgaccgccat
    26521 gccgctcaag ccgctgtaat ccacccccgg cgtggcggtg ccggagagcg cgtagctcac
    26581 cgtcaggttg tccgaagtcg tagccgcgcc ggtgctgctg gtgcgggaga gaaagagctt
    26641 cgagttgccg ctctccgtca tcgtcagcgt ggagaatgac gcgttcacga cgctgccgga
    26701 gtcatcatcc cggatgacga gcatggcctc cggctgcagc acatccacct ggtaggccgg
    26761 gtcgggcagg agggtgacga ccacgctttc cgcatcctcc tggagcgagt cattgatggg
    26821 cgtcaccgtg agggtggcgg tggcgttttt cgccatgctc agtgtgcctg tcaaagcagt
    26881 gtaatccgcg cccggagtcg cggtgccgga gacggtgtag cgcacggtga tgttgcccgt
    26941 gccagagccg gtggtggtga tgcggaagga gcccgcgtca ctcggttcgt tgcaggtggc
    27001 atcgagcaca cccacggcca ccacgggcag gtcattgtcg gaaatcgtca ccgtgtcgcg
    27061 gtgcggcgtg cccggcacat ggcgcgggtc ggcgcgcagt tgcagcacca ccgtttggtt
    27121 cggctcgccg tggtcgtcgt caatgggcac gatcagaatg tccgcgctcg actggcccgc
    27181 cgggatcgtc acctcccccg gcagcggcag gtagtccgtg ccgtgcagcg cgctgccgcc
    27241 ggccgtgtag ccgagtgtca aaggcgcgcc cgtgtcgccg gtgcgggtca cggtgaaggt
    27301 acccgtgttg ttgcccgcct cggaggcggc cgcatccgtg gcggtgagcg tgacagtggg
    27361 gatgtcatca tccgcgatgc gcacgatggc gctattcggt gtgcccacgc cgtaggccga
    27421 gttggaggcc acggtcaggc gcaccgtttc ctggccctcg atgagcgtgt cctgaagggg
    27481 caccaccgtc acctgcacgg cggactctcc cgccgggatg gtcaccgggc tgctgatcgt
    27541 ttgatagtcc tgcccgttgg cggcagccgc cgtgttcggc gcgccgtcca gggtgatgct
    27601 cacattcaag gacgcgtcca ccggcccggt gcggcggatt tcaaacacgc ccgcctcgcc
    27661 cgtctcgtgc gcgtcctcgt ccacggccgc cacgctcacg cggttgggca cgctgttggt
    27721 gccggtgtcc tggatgacca cagtggcggc cgccgctcca gtgatgctgt agctgttgct
    27781 cggcatgagc acgatgcgcg ccgcctccgg cccctcgcgc gtgctgtcat tgttcgcggt
    27841 cagggtgatg tccagcgtgc tggctcccgc tggaagggtg aaagtctcca gcggcgttag
    27901 ggccgtggtg ttcggcgcgg gagccagggt gtggtcggtg ttgaaagtcg cgtccccctg
    27961 caggtcggtg atgacggtca ggtccgccgc agtggagccg gtgcgggtga tgcggaaaag
    28021 gccggtgttg ccgcccgtct ccgcggcctc cgcgtccacg gcctcgatgc tgacgagcac
    28081 cgcgtcattg gcggtgtagt ccatgccggt caccgagccg ccgctgggca cattgatgtt
    28141 caccggggaa aagacatgcc cgcccttcac cggagtgagc agcacgctgc tggaagtgag
    28201 attggtgacc acgaagtcgc cgttcgagtc cgtccacgcc ccgcggaagc tggtgccggt
    28261 ggagccgttc tggatgcgca cctccgccac cggattgccc agcccgtcga gcacgcgccc
    28321 gctggccgtg cgcgtgcttg gcgcgcccgc ggtgatgagc acgagcttgc tcgtctcgcc
    28381 gcccttcatg tcgctgacaa tgcagcgcac cgcgtagtgg cgcgccgtgg tgaactgctt
    28441 gctcgtcgcc gccgcgttca cgccataggt ggcgtcgccg aagtcccaga aatacgccag
    28501 cggatcgccg tccgggtcgg tggcgtccac gctgaagttc accgtgccgt tcaaggccgg
    28561 cgtcgtcgtg ctggcggtca ggctgtggat gaccggcgcg ttgttgccgg ggaagaaacc
    28621 cagatgcacc tgcacatcca tgctctccgg cgtggtgccg cccttgccca gcggcgtgat
    28681 gtggacgccc gactcccggt cggaaaaggt gcgtccgatg acgacgggcg cgtcattttt
    28741 cccgtccgcc gagccgggcg tggtgtccag gagctggccg ccgcggttgc ctcccaccac
    28801 ctggtcctca ttgcccacct gattctgacc ccagcggctc cagtgcagca gcacgccgtc
    28861 gcggaaccag cggtggtccg cgttgtactt ctggcggaag tccacccagt aatcccgccc
    28921 cgcgtccttg gtcacccgca gcgcgtagcg ccgctcagga tcgagcaccg tctggtccat
    28981 cgggaagatg cggtaaacac cgctggtcgt ggccgcggcc acggtttccg ttggcagcca
    29041 gttcatcaaa ttcttgtgat gcgccatgaa gtgccctttg ttgcccgtga atgagccgct
    29101 gctgcccagc acatcgtagc tgtgcccgta ctcgccattg agaccgccgc cgatcacgtc
    29161 cctggtgagg gaggtgttcc agctcacgcc gatggtgcgg ttcgagtccc acgagttgcc
    29221 atgccacagc cccacattgt ggccgatctc atgggcaaag gtgccgatgc tcgtcgtgcg
    29281 cagccacacg ttcgccccgc ccacgctgcc caggccgcca aaggtgccgg ggccgttgcc
    29341 cgtgccgccc gtgtagatca ccgtgaagtg ttggtaattg tccgggttgt agcccagcgc
    29401 gcgcgccgcc tccttggcgt cggtcatgat cgggctgttg ctgcccgtgg ccgtgtagtc
    29461 cgccgtgtac caggcgcgcg agcgcggcag caggatcgcc ggcgtcacat ccgccagata
    29521 aaagaccttc ccaaagctca cctcggcgaa gtagtcgttg agctgctcga tggcacccca
    29581 ggcctgcgcc tccgtctgcg gctcctgcag gctgtcctca aaaatcaccc gcatgtagag
    29641 cagtttgcgc gggccgttgt tccagccgct ggccgccgga gcgctcagac ccgcggcgcc
    29701 gcccgtgccc ccctcccccg ccaccagctc ctccacaaac atgcggatgt ggccgccatc
    29761 gcacagatag acaatgtttt cccccgcctg caccgcggga gtctcctcca caatgggcgc
    29821 ctgcgtgttc tccaccgccg tgctcaggcc ggacaccggg caggtctcca ccgtcttcgc
    29881 cccggccggc gggcgctcgc cgatctccag cacgcgcgcc gggctttccg ccagcgccag
    29941 cacctcatcc accgccacgc catgcaccgg cactccgtaa agtgaattgt gcccgctgcg
    30001 ccgcccatag acataggtgc gcagctcctc gcccccgaag cgcgtgaacc agcgcacgac
    30061 cggctcatcc ggcccgctgc cctccgacac cgccagcacc tccacatccg ccgtggcatg
    30121 cacgcgccgc tccagctccg ccaccacctc aggcggcaaa gcctgccgca ccaccatcgg
    30181 caccgccccc tccaccgccg cgcgcggatc ttccacgatg agtcttttaa actccgcccg
    30241 ccgcgcccgc gccagcgcca caccctccgc caccagcgcc gccctccccg catcatccgc
    30301 ctccaggtag cgccgcgacc aagaactgaa atccctcagc accggcgccg ccgcctcctc
    30361 ccacttcggc ggcgcgggcg gccagtcccg caccggggaa aaaacctcct ccccaccgcc
    30421 ggcggcaccc gccgtcaaag cgcgcgcctg atccgcgcgg ggggcgcgtt tgtccaacat
    30481 gacaaaaagc catgcggcac aggccagcgc ggcgaggatt agaatgaggg gttttcgcat
    30541 gggttgggaa aaactgcccg catcctagcc aaagatgcct cattgaggaa gacaaatctc
    30601 ccgccgcatc ccgcaatccg catccccttc tgctcgtgga ggctcgaaga tgcctgtgga
    30661 ggcgaaggtt agtttgcccc gcgcccgatc cgggaagact cacgtcttcc tctactcccc
    30721 cgacggtccg ggggcttccc ccgacggtcc gggggcctcc cccggcggcg cggggaactc
    30781 ccccgacggc gcgggggcct cccccgacgg tccggggaac tcccccgatg gcgcgggg
//
LOCUS       DAOMYJ010000035        30638 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Prosthecobacter sp. isolate AMR_MDS_5629
            NODE_534_length_30638_cov_21.292842, whole genome shotgun sequence.
ACCESSION   DAOMYJ010000035 DAOMYJ010000000
VERSION     DAOMYJ010000035.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867850
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prosthecobacter sp. (wastewater metagenome)
  ORGANISM  Prosthecobacter sp.
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Verrucomicrobiaceae; Prosthecobacter.
REFERENCE   1  (bases 1 to 30638)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 30638)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.30
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:34:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,742
            CDSs (total)                      :: 4,684
            Genes (coding)                    :: 4,676
            CDSs (with protein)               :: 4,676
            Genes (RNA)                       :: 58
            tRNAs                             :: 55
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30638
                     /organism="Prosthecobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_534_length_30638_cov_21.292842"
                     /isolate="AMR_MDS_5629"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1965333"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..594)
                     /locus_tag="PLP58_05735"
     CDS             complement(<1..594)
                     /locus_tag="PLP58_05735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ08048.1"
                     /translation="MTHLIKTRALLGQLEAHGIDASRAMRGSAHQGGPDALLTAADGR
                     CWLVVIWGGTSLQAALFDEGAPPPLETVARALMNWRHEKGAAADLRMPDLLILAPALP
                     PGELPRGVWSEGDDTIHVITQRDCKKAAALAQSLHRLTASVLPAATIARWRAAAVPEV
                     RIDSPWKRKSIQRPADKLAAPLLLDYKQERCARLDLEP"
     gene            complement(591..2348)
                     /locus_tag="PLP58_05740"
     CDS             complement(591..2348)
                     /locus_tag="PLP58_05740"
                     /inference="COORDINATES: protein motif:HMM:NF024487.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA domain-containing protein"
                     /protein_id="HRJ08049.1"
                     /translation="MSAFSLDAETVLQDLRAFVAAEFEAQRLRFAEQRAKPRSERVED
                     GTCVDGLRFLRLDEAGRAVFRHEGNDSRLREGDLVTLRQSDEGDEMLVVLFREEAGEM
                     WLASERGLKTAMFAETGGWFIDEAFLDLESHYLTAFDRLPNSAIGQECLLPLLMGKQK
                     PELDEEEFNGAMEDLQKQPQAWEDAQRDAIASCLATERCCLVQGPPGTGKTRVLSQVV
                     RQLVERGERVLITAFTHRAIDNALSATAREIDDRDRVARICAATHRREENFDRYEFFA
                     ASPLAAQSGGWVAAATPFALRKRLPGVEFDTIVIDEAGQMTTALAIMAMLAGRKYLLF
                     GDQQQLGPVVMSRSRREAEFVGVFHALRAQSKHGTCLDVTYRLNDKLAEWPSENFYHG
                     ELVPAPLAAARRLACPTSSHEPEWIREVISSEKPLIWLQHTSEASRTVNSAEVAAAAE
                     VLRALHRGGVKPEDIAVVTPYRKQARAMRRQLETLMPETSWRGCVIDTVERMQGQERE
                     VIVFSMCASDPSFIRRQAEFLFDPRRLNVAVTRARSKLIILASESLLHTDLQDTELAE
                     HQSLLRSLSRCAHSVSFPA"
     gene            2418..3494
                     /locus_tag="PLP58_05745"
     CDS             2418..3494
                     /locus_tag="PLP58_05745"
                     /inference="COORDINATES: protein motif:HMM:NF012904.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HRJ08050.1"
                     /translation="MPSHLTRRQLLGTALSGTTAILAPHHLHAAEADPKVRGPFPILS
                     TPFTESGAVDYEVLAEQARFVDWGGCPGMIWPQSGDSVDLLTMEEKLRGMEVLAETAK
                     SSKTSSLCLGVQGRDTEEMLVYARHAEKLAPAAIISRPPDLGRTEDDLRQYWRELAKV
                     AKRPVIFQTTGGVAYKGPVPSVKLMIELAKEFPHFGYIKEEAAPIIARTKELCAAKPV
                     IRRVFSARGAHGWLYESHLGTEGLITERAVYADVLTKVWNLMQSGDDPDTARDIHAKL
                     MLLFNLGQHLPGGDLRGFQLHLWKKRGVFKNMISRQYGPNRSIPDKPIFSELKLTADE
                     IAEIDLRFEALRPYLKAERMAPAK"
     gene            complement(3604..4818)
                     /locus_tag="PLP58_05750"
     CDS             complement(3604..4818)
                     /locus_tag="PLP58_05750"
                     /EC_number="3.2.1.18"
                     /inference="COORDINATES: protein motif:HMM:NF024488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exo-alpha-sialidase"
                     /protein_id="HRJ08051.1"
                     /translation="MIIRPLLFIATLTTVSRLTEAADSPIMLSGPWMPENTHDIDFAA
                     LPRVPGEHVVISDVRAKGNDPSKLDKKGGGVNQHNYLAHHDGQFWAMWSDGPGIEDRV
                     GQRVKFATSPDGLKWSEPKFMTPIPPNSGPDSPHYATRTAKGMRWISRGFWNRDGELL
                     ALASLDEAAGFFGESLELRAFRFDKAAGEWRDAGLVFKNTINNFPPIKLRTGDWMMSR
                     RMFDYKTSGVHFLVGGVKSLSDWQSFPVLGSAAELKAEEPDWWILPDNTLAAVFRDNR
                     RSGFLYRALSADDGRTWSTPIKTNFPDATSKISGLRLKDGRYVLVSNPNPKKRDPLTL
                     SISNDGLVFTKMLYLVGGRHIDYPHVIEHEGHLYIAFAGGKQTVEVLKVMLSDVDAVK
                     MPTTPLRVGEAK"
     gene            complement(4851..6446)
                     /locus_tag="PLP58_05755"
     CDS             complement(4851..6446)
                     /locus_tag="PLP58_05755"
                     /inference="COORDINATES: protein motif:HMM:NF024240.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HRJ08052.1"
                     /translation="MRSGLSLITFALLCGQSLAQDIVIYGGTPGGIASAISAARLGRE
                     VTLVEYHDHIGGMTTSGLGKSDIENRAMIGGIFKEFVAGVREHYITKYGPEHEHTKLC
                     QDGYYAEPHVAEKVFEAMIAKEPNITVLKGWRLKSADVSDNKLRGITIVKRQNDDEKQ
                     LEAKVFLDATYEGDLYAAAGARFRLGRESREEFDEPHAGVVYFDYQAHEFLPGTTGKG
                     DDALPAYTYRLCLTKDPANAARMTEPPPGYDRRDYLGYFDDLKAGRLAGPKVFKPGRG
                     YNPKHFDTLVRALSVADIPNNKCDVNINPRPLGFPFPEENRGYIEGDEATRARIRQRH
                     RSLVLGLLWFLQNDPEVPEAHRALANEMHLPKDEFTDNAHFPWQLYVREGRRLIGEYT
                     LTQHDITGKGQQPKHHDDSIAVGEFPIDSFPCRKRQPGDTLVLEGYLGMLDYITRPYE
                     IPYRIMIPEKMDGLIVTVAASTTHVAFSSIRMEPTWMALGQAAGAAAHLAIQNEVEPR
                     QVPTEALQNLLLKQGQVIRHATH"
     gene            complement(6645..7292)
                     /locus_tag="PLP58_05760"
     CDS             complement(6645..7292)
                     /locus_tag="PLP58_05760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ08053.1"
                     /translation="MKTILILLALSMPITGLSASDEASAAKTIPFKLTGKTRVKNEKH
                     EFWLPHPEGPLSVLLLPEDPRTLCAKSSPEGAAAMKDMEGLQVGRFHMSGQCVADPSG
                     KFHVVTVVETMMPAGEGVVRTEEREVTGKVEVKGDPAKGQGGGSMIIHASDGTEYFIP
                     PKKFDPKPDGLDYMSAAKLAGQQVTMKASVQIHREPPHRVAKVIMFRVSTAQTAK"
     gene            complement(7319..8695)
                     /locus_tag="PLP58_05765"
     CDS             complement(7319..8695)
                     /locus_tag="PLP58_05765"
                     /inference="COORDINATES: protein motif:HMM:NF019222.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1552 domain-containing protein"
                     /protein_id="HRJ08054.1"
                     /translation="MNSSMLNRRRFLNRLSLCAGAPVLAPLLDRVLLGAEGGKPPLRF
                     LFVVEGNSVPPKQLCPAGIPFVEREQRNAFTEHSLLDAELPTALKPVAAYRDRLTVIQ
                     GLSGRMCSGGHSSDHGALGAYHANHGRNLQAATVDCVIGQAHPGIHENLVLGISSDAD
                     KVVDFNCSASGRGRSRATLLHPSVAYTQLFGAVAKGEAGAAFQARRHLLDHMRGDIRR
                     ARSALGSLEKEKMDAYLSSFETLGTTSQRLVEARDRIAKVDPGMTDKYSSQVETDRLD
                     AHFELATAALIGGMTNCATIASGVGFPNFNITFTGLGIQTGKHPIGHALYNEDDTTGW
                     DQSQKIRAFHFQLIARTMDKLKTMPEGGGTMLDNTVIVYLSDGAETHHSRCFEWPFVV
                     LGNASGRLKAGRYLQFPDYGLKGHRTINALHETLLHLAGASQGLFGSLDPNLDKDMHR
                     GPLDPLLA"
     gene            complement(8710..10725)
                     /locus_tag="PLP58_05770"
     CDS             complement(8710..10725)
                     /locus_tag="PLP58_05770"
                     /inference="COORDINATES: protein motif:HMM:NF019259.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1588 domain-containing protein"
                     /protein_id="HRJ08055.1"
                     /translation="MKSFHTLSCVILVFSLCLASSSVWAADPAVPSFLKTYCADCHNP
                     VKMKGKVSLTPLIENPASADPSLLQAVLDVVTSREMPPEDEKQPPSEARTAFLAAGSP
                     LMKEVALNRAKEAELPGHGNLVPHAVLFTEPEVRRAATPARLWRMSPHLFMPRANQLT
                     GARFLEPRRNQGGDGLHPAFAYMTPPHAFRDDAGQHLFEEATTELLFNVCWDIAGLQM
                     QGKRVAPEIEALLQNKEPSDADWHPIIRRQYQMGLHRQPHDSEIAELMPLVEQTQRDS
                     GRNMALQALLAAVLMKPEAVYRFEIGQGTPDAHGRVMLAPQELKHAIAYALTDSPPDV
                     SLEHAAKEGRLAGRDDVRREVERLLGDFETTSPRLLRFFQEYFEYTAAPEVFKDHRQA
                     NAIFARERVEGADEMVLFILERDEDVLRRMLTEDRVFISDASEQREPVEKRFRRSLHP
                     DYGFPPEWIWEQEQPVQPPQGRRSGILTHPAWLLAFSDNEKNQAIQRGLWVQTKLLGG
                     SIPDTPVGVDAKLPADPHLTLREKMKQVTSATYCWSCHQKMDPLGLPFEQFDDFGAYR
                     QKELERPVVTTGEIKIGVPEIDGPVADPFEMLARLSTSPYVEQVFVRHVFRYFLGRNE
                     TPDDAPTLMDAHEAYRKNNGSMRALAASLLTSDSFIYRRLPETLATK"
     gene            complement(10722..12071)
                     /locus_tag="PLP58_05775"
     CDS             complement(10722..12071)
                     /locus_tag="PLP58_05775"
                     /inference="COORDINATES: protein motif:HMM:NF016648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FecR family protein"
                     /protein_id="HRJ08056.1"
                     /translation="MSTDWNDLIQRHIAGLTTEAEAAQLQQALKADDSLADLYIRHIE
                     LDVALEAKAASAEATRELLTAPVADEARRSTRWLAWRPLAAAAAVALLGMAGTLWLRT
                     IPWPRGASATLVSADNARIVGVDGELIAGRELRLKELRLETGRLEIALASGARLEMSA
                     PVEGEFFDDMRLRLDSGGVNADVGEHGKGFTIETAAGEIVDLGTRFGVQADRSGESRV
                     AVFSGKVEVHSRQPRMGGVPVTLTEGMAARFSAQAGLRGWRQVAVAAQEAGLAAAHYT
                     GIVERVHDNLGDEELEPFYGVVQRGMAPGVLAFADKPNPRWAPRPGDEVPASLLGADL
                     IRTYHQFRRRGSYELTVTLREPAVVYVLIKAQDQLPEWLAEKFSPTGETVIAGPLQVG
                     IASWPDAILDEEGRPYFKFAVWKANAPAGEFTLGTARSAERQLMYGLAIQPQKMSPP"
     gene            complement(12068..12592)
                     /locus_tag="PLP58_05780"
     CDS             complement(12068..12592)
                     /locus_tag="PLP58_05780"
                     /inference="COORDINATES: protein motif:HMM:TIGR02989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HRJ08057.1"
                     /translation="MQPPLDPQHAQFLRLYAEHEAALQVFVRSMLPCRDETDEVMQQV
                     IVVLWQKFETAQDFKKWAFGVARLEVLRFLQSRKRDRHVFDETLVNRLADRAVLHAAE
                     VMPRREALEHCLQKLPPSQRELVLTAYTKGTRMDELATRRGQTPMALCKLLHRIRIAL
                     LDCVERTLAREEAA"
     gene            complement(12694..12966)
                     /locus_tag="PLP58_05785"
     CDS             complement(12694..12966)
                     /locus_tag="PLP58_05785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ08058.1"
                     /translation="MVEFKDAMALHPTKYSHQESFIQACLGNGTTESPFSVAGVLTQV
                     LNLGTIAEYLNVDLQFDPQTKRLIGSEEANARRAGPAPRAGWADCC"
     gene            complement(13064..13234)
                     /locus_tag="PLP58_05790"
     CDS             complement(13064..13234)
                     /locus_tag="PLP58_05790"
                     /inference="COORDINATES: protein motif:HMM:NF024240.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HRJ08059.1"
                     /translation="MRFGRPLIALAFLCGQLLAQEIVIYGGTPGGIATAVSAARLGLE
                     VTLVEYHDHIGA"
     gene            complement(13328..13801)
                     /locus_tag="PLP58_05795"
     CDS             complement(13328..13801)
                     /locus_tag="PLP58_05795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ08060.1"
                     /translation="MCRFEGERGGWKRDRGDLWLCLPLHDAGAAEIQRRLHVPEGWEP
                     ASKDGAWSLYERAGQIIGVFSTDTLGLFCLLGKGDGETQLRQLIAANPDASQLKRRFV
                     WPGGGTLDYDLHAEKDRWVIVSEDDKPVNRDHGAWPLMAGEVPGFNGGSDQTLEN"
     gene            complement(13788..14399)
                     /locus_tag="PLP58_05800"
     CDS             complement(13788..14399)
                     /locus_tag="PLP58_05800"
                     /inference="COORDINATES: protein motif:HMM:NF016612.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="neutral/alkaline non-lysosomal ceramidase
                     N-terminal domain-containing protein"
                     /protein_id="HRJ08061.1"
                     /translation="MKLWLPLLLSLFVIAAAPVLETAAWKAGAASAKITPEKSMWMAG
                     YAARTKPSESVELDLFAKPFALTDQEGGRFVLVTLDLIGVPRNLRLAVAERAKKEHGI
                     EPANLAINASHTHSGPELRTSRLHGADDVAQRELETAEYTTALENTILRLIGEALEKS
                     VPAHLDYSHARCGFAMNRRTPDGKGGWSNFPNPAGPVDQSVPV"
     gene            14446..15348
                     /locus_tag="PLP58_05805"
     CDS             14446..15348
                     /locus_tag="PLP58_05805"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HRJ08062.1"
                     /translation="MRSLILLPLLTLSAFAQQPKPAPTKLAPEITASLEAHPGLVYAR
                     YGQREMQLDLWKPKTAPQPLPAIICIHGGGWYKGERSNMANLAQALAAKGFVAATISY
                     RLSGEAKFPAAIQDCKAAVRFLRANASKFGIDAKAIGVTGLSAGGHLAALLTTSGGVK
                     ELEGDGGHADQSSAVQAGIAMGAQSDLESARIGELSSKPDDPFYRTFLGDSQAKVPQT
                     YALASPRHHLDKTDPPLLFMTGELDDPSTHADDARADLAKLTIPTGLEVIPQAPHSFL
                     GQQKAFDACVNACVAFFTKHLLKN"
     gene            15427..18693
                     /locus_tag="PLP58_05810"
     CDS             15427..18693
                     /locus_tag="PLP58_05810"
                     /inference="COORDINATES: protein motif:HMM:NF025030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HRJ08063.1"
                     /translation="MLLCLPHAVHAAEDAGISELLTWTAEAPDWQEHLKRRMSEGWKP
                     PARVSSTPDQRDDLATLIDHWKSHPYGHREEKPDAATRERLLKACEEQPAVFESVGFR
                     FEPADAGVPQRIQQLYQRMPATSEEEKKAKAKARDWLMCEAGLLRPELKQEAEAAFQH
                     ALERTRRSYLDAFLRHEPAEGEKLLLQHAQDTEPASRLLALLRLFDQDAARWRSGIQK
                     LVESNAAPALRIEALKALAAKRWPGSDAWLLGLFKHAELGYLSEDYHSSEPFAEIIKA
                     APDVWIPRVIQLVGNKDRAVHDNAVRCLAQFHLSETREDALKPLLPWLTNPAWALDPE
                     HDGRLRLIQSLAQISLPQSVPGLIYVLEHDSGYWRSAAADALAHYKAREAVPALRKAL
                     LVENDAHHQRSIASAMLKLNVFSSEELVEGIESLVEQTLTEEKRSALSALSDPFAPSE
                     MPKLPRPLLARIAIGQCTHDLDADLREGVAKTLLQRTKALRPKNSALADALELHISDW
                     ETPANHELLRQRLLSGNLSAEWVWRLLESAPTIENAPPVSQAIIAVAHRDGKAAAKML
                     ADPHADAQAMLLACARLKRLVLPVKEVQSLLASKHILVKRAALLYLEALDTAEARELV
                     WSHFPGEARILGARMGYDPGHFSFRGLDQWEDVLRQRVLAPGGADEIHALLSAGYWGN
                     AGQLWIEKRPDGAWLVNAEDRGRQRVRKISQDEYAELLRYLKEHQVDELPTFDTGTQD
                     GLQLEHVHLTRNGGKRVFMNNPDSGADGAPEVVYENLVTIYRDLIRDTSRLSVEYRGV
                     SDVTIEIAAEKMPVKAVWGRAGQLFVHARLPGTQRDRWCQLDGTPAQGEFPSIVDPCD
                     AGSDSHLQKAAWLSNGIRVGDHPQTKKSGLWHEKGEWISDGVFADPVVSADGKVVVVS
                     RARGKHWGEPNDVVRIELGTKKVLPIDLPSADDFSVITRLPDTGKILLFRSRNEPVPG
                     IKPKEGPQKPEYHLLEASTGELERVKGEFQPLHDETWRPLQNTAKQGIVWAALPSMEK
                     GRQTGTLIGRYDLSSFHFETFMKVPVLQFSSMDFWVEESKKRVWIVVNNDLLSLPMAD
                     "
     gene            18693..19184
                     /locus_tag="PLP58_05815"
     CDS             18693..19184
                     /locus_tag="PLP58_05815"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012419777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HRJ08064.1"
                     /translation="MNTPTYFTTHASPLGEIILAATENGLCGLYFKDHTPAPKREGWK
                     RDNGPRFDAALAWLDAYFAGEKPRKMPAHAIASGTPFQRSVWEALRDIPRGKTTTYAE
                     LAAAIGSPKAVRAVGAAVGRNPLSILIPCHRVIGRDGSLTGYAGGLARKKRLLALEGL
                     KFD"
     gene            19194..19559
                     /locus_tag="PLP58_05820"
     CDS             19194..19559
                     /locus_tag="PLP58_05820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009965488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MGMT family protein"
                     /protein_id="HRJ08065.1"
                     /translation="MAKSPAYARIRAEVLRLVALIPEGKFTTYGSIAVHMNVVARHVA
                     TVLSRLTEDESKALPWHRVVSRDARISPNMDAKLAAKQKRLLKAEGLKIGRDGFIQDA
                     GAHFHVVGVRRNIRWSAME"
     gene            19624..20154
                     /locus_tag="PLP58_05825"
     CDS             19624..20154
                     /locus_tag="PLP58_05825"
                     /inference="COORDINATES: protein motif:HMM:TIGR02989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HRJ08066.1"
                     /translation="MPDSPPNMENLVLLLTQHQEPLFRYIFSLVPCEADARDILQETS
                     VALFRKFDQYDATRPFLPWAYRFAYLQVQKHRERSARLPLLFSEDVMDLIANERAHIE
                     PRLDVRLRLLDVCLGKLTPQDKELVTRRYALREGAEEMMRRFDMSRRTLFRNLEMLRQ
                     RLHECVTRQLESEGLA"
     gene            20151..21674
                     /locus_tag="PLP58_05830"
     CDS             20151..21674
                     /locus_tag="PLP58_05830"
                     /inference="COORDINATES: protein motif:HMM:NF024777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FecR domain-containing protein"
                     /protein_id="HRJ08067.1"
                     /translation="MSLESLIHAQLDGEITPEQHARLDALLREDWQARRLYLELADLH
                     ARLLQQPQIGTGRLPQAEKPPGLRRWRKLLPVLASAAVIAVGFLMWPPESTRSEATFS
                     GVAMLSQMLDAEFAQNTLRSGDTLAPGTIKLTKGLAQIEFFSGATALIEGGAEIEVIS
                     AWEARCVSGRVRVHVPPAAKGFLMHAPGMKLEDLGTEFALNVKDGTSAVHVFEGEVIA
                     HTDQPPASLKEGMTLGQTDAGFLPIGDVHDLVRQRQERRHAEWQQWSQEARKDPRLIA
                     YYTFKHWPDDRWDRLVNNFTEPRRPNRAGGAVGARWTQGRWPGKEALEFKRPGDRVRL
                     NLDDTYDALTLACWVKVDSVDKKYNSLLLTDGYDNGEPHWQIYEDGSLMFSIMYRPAD
                     AAKNAKYNQMYHSKPVFTADRLGRWHHIAVTYDSQSGEAVQYFDGSEVSREVHPLHQP
                     GRDITCGPCEIGNWGLPTEGHQFPIRNLNGVIDEFAVYGAVLGADEVREVFEAGKAE"
     gene            21762..22970
                     /locus_tag="PLP58_05835"
     CDS             21762..22970
                     /locus_tag="PLP58_05835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009961003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tm-1-like ATP-binding domain-containing protein"
                     /protein_id="HRJ08068.1"
                     /translation="MASTIAVLGTLDTKGPEHAYVAELIRQRGHQTLLIDTGTGAAPT
                     AAPDIPREEVAAAGGVDLPGILARRDRGEAVTAMSGAAAALLERLAVSGRIQGVISLG
                     GGGGTAISTAAMRALPIGFPKLMVSTLAAGNVAPYVGTKDIVMLPSIVDVNGLNRLSR
                     ALLARAAGAICGMVEMEPPAADDKPLLCASMFGNTTECVNAARKILEDAGYEVLVFAA
                     TGTGGRIMESLIESGMISGVLDITTTEWADELIGGVLNAGPTRLEAAGKAGVPAIVTP
                     GCLDMVNFGEPATVPEKFKDRLFYQHNPQVTLMRTSAEECAQLGRILAGKINAYTAPV
                     TVLIPRKAISVISAPGQPFHDPEADEALFSSLKSHLRVDIPVIEMDCEINAPEFAAAC
                     ARTLLQQMQG"
     gene            23061..24158
                     /locus_tag="PLP58_05840"
     CDS             23061..24158
                     /locus_tag="PLP58_05840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009963206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 N-terminal
                     domain-containing protein"
                     /protein_id="HRJ08069.1"
                     /translation="MSPGQLLLTGVPGPELDPDTAARFKKLQPGGFILFGRNIKSPGQ
                     LRKIVDDLRDLSDTEPFITIDQEGGRVSRLRLIGQEPPNAQQLRDKGDPALIKQHGRL
                     TGQLLRLFGFNLDLCPVLDISYDDTADNSLKGRCWGKDPQQVITNAGIFNSALRKEGI
                     LTCGKHFPGYGPAECDPHEFLPVITKSRADMEKSELLPYSALLPELDSVMTCHSNYTA
                     YDPGRGRWPASLSRNICTRLLRDQLGFEGLAMTDDLDMGAILNEVTFEQAIQEAVRAG
                     NDLVMICHRVEMVEQARQHLEGVERPVLHDALLRIEKTKKRLVKPDAFSLDRFQQINS
                     GIWDLRVATLGEERAQILSVEDGKRSPVELY"
     gene            complement(24213..26003)
                     /locus_tag="PLP58_05845"
     CDS             complement(24213..26003)
                     /locus_tag="PLP58_05845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ08070.1"
                     /translation="MQPPATASPPLLRLAVYALAAGMALTHVFVTFQGLRSAQGMEQA
                     QLARELARGHGWQTLVIRPQDWARLIAHGADASPRTMPDISQPPVQPLLLAPVFKLLE
                     KWQAYRPLKTGPIYLLDRAIACIGVTALLLTLLWTHGAARRLFDEKIAAVSVLALLFC
                     QPLWHLAVSGSPAALLLPMAALSLRLHAAAASAAAEGARAWPALLGMGLLAALMALTH
                     WMAAWLVLGLGLSLALTFPARRFTAALLVVTPPLLALGAWGFWMFQRCGDPLGGVKSL
                     LQAHLLIGDPDLLHRAFSLTAPPVDMAALLRKAAENSLQLAGGIFSHLGGSLPALFFF
                     PALLHRFRNPAAASLCRALAITGLGILTGSILLGLPGGFEDEQNLIPVLVPALTIFGA
                     AMLAVLWARLYPLANAASSFWRRLGFAALAIGLTAVPMLVTLPPQLKLGLTLRARMFP
                     HWPPYAPDRVQIVQRLLEPGEVVFADAPWFVAWYADLPALWIPARRDDYPAMKAKAEA
                     AGAKVAGFIVTPLSARARHLHEVFSGPWREWPDLVFRGIVLAFDREFPPRPDFEFKVP
                     MPLIAIIVGKEENKALPMIFYTDRPRTLRD"
     gene            complement(26172..27539)
                     /locus_tag="PLP58_05850"
     CDS             complement(26172..27539)
                     /locus_tag="PLP58_05850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ08071.1"
                     /translation="MIVKENELTPKQQNLWKKGLLAIEQKNWEYAVGLILPVVQELPG
                     FLDGRMALRRAEGEVAGGGRKFSFGGGLFKGTKKDPWEAIADLEENVFQKDPFNPGAN
                     QQLYDLAMKVQFPDLAAFALETIRMGQPENTKNMHTLAQHYMAHDQPEKASEVYRAIV
                     KVDPRDITAVKGEKDAAARSSILRQGWDQEGDFRKAMKNAEEAGILELLNKQGRTREQ
                     TESLLARVVDEYNQDQTNINTVKAMAQLYDELDQSDNALMFYEYALTLNPGDVALQRR
                     TELIRDKVRDIHIAQMEADIEANPGAPDIEEKRAQLAQIRMERAQTAINEARARVERN
                     PTDKQVRFDLGQAYFNAGMHTEAIPELQQAKSNPNIRIKAMLLLGRCFERKNMNDLAQ
                     SALAEASKELAIMDNTKKEILYELALVNEKMGRQEQYLEALKEIYNADYGYRDVARRV
                     ESSYQ"
     gene            complement(27546..27755)
                     /locus_tag="PLP58_05855"
     CDS             complement(27546..27755)
                     /locus_tag="PLP58_05855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ08072.1"
                     /translation="MRLLSKILWLLAFLAATFCWMVLFEHGFTMKGFSQGVREEWQAL
                     MQLVSPEKSAAPAQEPPTPKSTPSK"
     gene            27873..29057
                     /locus_tag="PLP58_05860"
     CDS             27873..29057
                     /locus_tag="PLP58_05860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009961841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HRJ08073.1"
                     /translation="MTPLHHLRAGIIGTGFIAPVHIEALRRLGVQVTAVCGSEKSASA
                     VASLWGIPQVFGGYDHQALHRSPDVDVVHITSPNKAHVEQSLDALSQGKHVICEKPLG
                     MTSAETARVVEAVKKGGRKGPVFAVNYVCRFFPAVLQMRAMVARGDLGRIIHVQGRFF
                     QDWLLHDTDYNWRLLASEGGKLRAVGDIGTHWMDAVSFVLGARATRVFAHIETFHKIR
                     LRPKSEVQTFSKTAAAAVKLQPYKVDTEDFGSVLLKFGKAKHGYCDGVHANALISQVA
                     AGSKCSLGLSIHGTLGSVQWDLQQPNEILLGRRDQPNQILQRGTPGFLEDVANFTDYP
                     GGHPEGFSDAHKMHYRAVYEHIASGKKTPALFATAEDGHHEVRLCEAILHSSRARTWV
                     KV"
     gene            29085..30512
                     /locus_tag="PLP58_05865"
     CDS             29085..30512
                     /locus_tag="PLP58_05865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009962885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ08074.1"
                     /translation="MPGPNLIQQENARPGSSDWQLTRVRLDKSAGFRSPAIEGYASRQ
                     SVKAGDTLDIHVSTRPAARFKIEIFRTGYYQGKGARLMTQLGPFEGREQPVPDMQPGT
                     QLMECVWEKTATLAIPPDWLSGVYLGRLTTLPDSADEPYWQSYIVFIVTDDRPADILL
                     QCSDNTWQGYNRWPDATSLYIHPKGGQGPWPHVSFDRPYAKYAQIYENPQSIGSGEWL
                     CFEFPMAYWLEQHGYDVTYCGNSDMITPDRGLKCKAFISIGHDEYWDIRQYESVVQMR
                     DSGVNLLFLSGNSVCWVTPFSPNAAGIPRRRIFRSGPYGGDYEYAVHREQNHGPFPHR
                     GPDEGYLMGARNVRPVNGGGDWHCVKPEHWIFEGTGMKAGDSVPGLIGWEYHGDPPAD
                     LPGLEVIAAGTAWQGGRVPQQWTATLYPGPKGNYVFNAATIWWGLGMSKPPGTMPVWS
                     HYSRPHGPDPRVQKITANLLQRALR"
BASE COUNT         5716 a         9937 c         9500 g         5485 t
ORIGIN      
        1 gggttccaga tcgagacgtg cacagcgctc ttgtttgtag tcgagcagca gtggcgcggc
       61 gagcttgtcg gcgggacgct ggatgctttt gcgtttccac gggctgtcga tgcgcacctc
      121 cggcacggcc gcagcacgcc accgcgcgat ggtggccgca ggcagcacgg acgcggtgag
      181 gcgatgaagg ctttgcgcaa gtgccgcagc ctttttgcaa tcgcgctgag tgatgacgtg
      241 aatggtgtca tcgccttcac tccagacgcc acgaggcagt tcacccggcg gcaacgcagg
      301 tgccaggatg agcaaatcag gcatccgcag gtctgcggca gcacctttct catgacgcca
      361 gttcatcaaa gcacgcgcca ccgtctccag cggaggcgga gcaccttcat caaagagcgc
      421 cgcttgcagc gaggtgcctc cccagatcac caccagccag catcggccat ccgcagccgt
      481 cagcagcgca tccggcccgc cttgatgagc actgccacgc atcgcccggc tggcatcaat
      541 gccatgagct tccaactggc cgagcagggc gcgtgttttg atgaggtggg tcatgcggga
      601 aacgaaactg aatgcgcaca ccggctcagt gaacgaagca gcgactgatg ctccgcgagc
      661 tcggtgtctt gcaggtcggt gtgcagcagc gattcactgg cgaggatgat gagctttgag
      721 cgggcgcggg tgacagccac gtttaggcgg cgtggatcga aaagaaactc cgcctgccgc
      781 cggataaagg acggatcaga ggcgcacatg gagaacacga tgacctcgcg ctcctgcccc
      841 tgcatgcgct ccaccgtgtc gatgacgcag ccgcgccagg aggtctccgg catcagcgtt
      901 tccagctgcc gacgcatcgc acgggcttgt ttgcgatacg gggtgacgac ggcgatgtcc
      961 tccggcttca caccaccgcg atgcagcgcc cgcagcacct ccgctgccgc tgcgacctcg
     1021 gcagagttca ccgtgcggct ggcctcactc gtgtgctgca accagatcag cggcttttcc
     1081 gatgaaatga cctcgcgaat ccattccggc tcatgcgaag atgtcgggca ggcgaggcga
     1141 cgagcggcgg cgagtggcgc gggaacgagt tcgccatgat aaaagttctc cgaaggccac
     1201 tcggccagct tgtcattgag gcggtaggtc acatccaggc aggtgccatg ctttgactgt
     1261 gctctcagcg catgaaaaac gcccacgaac tccgcctcgc gacgtgagcg gctcatcacc
     1321 accgggccga gctgctgctg atctccaaac aaaagatact tccgtcccgc gagcatcgcc
     1381 atgatcgcca gcgcggtcgt catctgcccg gcctcgtcga tgacgatggt gtcaaactcc
     1441 actccaggca gtcttttccg caaagcgaag ggcgtggctg cggcgaccca gccgccgctt
     1501 tgagctgcca gcggactcgc cgcgaagaac tcgtaacgat caaagttttc ctcccgccga
     1561 tgagtcgccg cgcagatgcg ggctactcgg tcacgatcat cgatctcgcg agccgtggca
     1621 gagagcgcgt tgtcgatggc acgatgcgtg aaggcggtga tgagcacccg ctcgccacgc
     1681 tcgacgagtt gacgcaccac ctgcgacagc acacgcgttt ttcccgtgcc aggcggcccc
     1741 tgcacgaggc agcagcgctc cgtcgccaga cagcttgcga tggcatcccg ctgcgcatcc
     1801 tcccaagcct gcggctgttt ttgcagatcc tccatcgcgc cgttgaactc ctcctcgtcg
     1861 agttccggct tctgtttgcc catcaacaaa ggcagaaggc actcctggcc gatggcgctg
     1921 tttggaagac gatcaaaggc agtgagataa tgactctcca ggtccaaaaa ggcttcgtcg
     1981 atgaaccagc cgcccgtttc ggcaaacatc gcggttttca atcccctctc cgacgccagc
     2041 cacatttcac cggcttcttc acggaagagg accaccagca tttcatcgcc ttcgtctgac
     2101 tgtcgcagcg tcaccagatc accctctcgc agccgtgaat catttccctc atgtcgaaaa
     2161 acagcccgac ctgcctcatc gagccgcaga aagcgcaggc catccacgca ggtgccgtcc
     2221 tccacgcgct cgctgcgagg cttcgcccgc tgctccgcaa atctcagccg ctgcgcctca
     2281 aactccgccg ccacaaaggc gcggaggtct tgcaggacgg tttcggcatc gagagagaag
     2341 gctgacatct ggaaatctac acaccgggca gccgaaatgg aaagctcggg aagcaagttt
     2401 gagttttctt catcaccatg ccaagtcacc tcacacgtcg ccagctcctc ggaactgccc
     2461 tttccggcac gaccgccatc ctggcccctc accatctgca cgccgccgag gccgatccga
     2521 aggtgcgcgg gccgtttccg attctctcga cgccgttcac ggaatcgggt gcagtggact
     2581 atgaggtgct ggcggagcag gcgcggtttg tggactgggg cggctgtccg gggatgatct
     2641 ggccgcagtc aggggacagc gtggaccttt tgacgatgga ggaaaaactg cgcggcatgg
     2701 aggtgctggc ggagacggcg aaaagctcta aaacctcgtc gttgtgcctc ggcgtgcagg
     2761 gacgcgacac agaggaaatg ctcgtctatg cgcgacatgc ggaaaagctc gcgcctgcgg
     2821 ccatcatctc gcgtccgccg gacttgggac gcacggagga cgatctgcgg cagtattggc
     2881 gtgaactcgc gaaggtggcg aaacggcccg tcatcttcca aacgaccggc ggcgtggctt
     2941 acaaaggccc ggtgccctcg gtgaagctca tgatcgagct ggcgaaggag tttccgcact
     3001 tcggctacat caaggaagaa gccgcgccga tcatcgcccg cacgaaagag ctgtgcgcgg
     3061 cgaaaccggt catacgtcgc gtcttcagcg cgcgtggagc ccatggctgg ctctacgaat
     3121 cgcatctcgg caccgagggg ctcatcaccg agcgcgcggt gtatgccgat gtgctcacga
     3181 aggtatggaa cctcatgcaa agcggcgatg atcccgatac cgcgcgtgac attcacgcaa
     3241 agctgatgct gctcttcaat ctcggccagc atctgcccgg cggtgatctg cgcggcttcc
     3301 agcttcacct gtggaagaag cgcggcgtct tcaaaaacat gatctcccgc caatacggcc
     3361 ccaatcgcag catcccggac aaaccgatct tctcggagtt gaagctcacg gcagatgaga
     3421 tcgccgagat tgatctccgc tttgaggcgc tgagaccgta tttgaaggcg gagcggatgg
     3481 cgccagcaaa gtagccatct tgctccgcaa gatgagctga aggctgccgc tggatcggat
     3541 cgccttcgaa tggtgacgat tggagactgc ggggtgccag gctcctctcg cggagcgaga
     3601 gggctacttt gcttcgccaa cgcggagtgg cgttgtcggc atcttgaccg catctacatc
     3661 gctcagcatc actttgagca cttcgacggt ctgtttgccg cctgcgaagg cgatgtagag
     3721 atgtccttcg tgctcgatga cgtgcgggta gtcgatgtga cggccgccta cgaggtagag
     3781 catcttggtg aagacgaggc cgtcgttgct gatggagagg gtgagtggat cgcgcttttt
     3841 tgggttggga ttcgagacga gaacgtagcg gccatctttg aggcgcaggc cgctgatttt
     3901 cgaggtggcg tcggggaagt tcgtcttgat gggcgtgctc caggtgcggc cgtcgtcggc
     3961 ggagagggcg cggtaaagga agccgctgcg gcggttgtcg cggaagacgg cggcaagggt
     4021 gttgtcgggc aaaatccacc aatcgggttc ttcggccttc aattcggcgg cgctgccgag
     4081 cacagggaag gattgccagt cgctcaatga cttcacgcca ccgacgagga agtgaacgcc
     4141 gctcgttttg tagtcgaaca tgcggcgcga catcatccag tcgccggtgc gcagtttgat
     4201 gggcgggaag ttgttgatgg tgtttttgaa gacgaggccc gcatcgcgcc actcgcctgc
     4261 ggctttgtcg aagcggaagg cgcggagttc gaggctttcg ccgaagaaac ccgccgcctc
     4321 atccagcgag gccagcgcga gcagctcgcc atcgcgattc cagaagccac gcgagatcca
     4381 gcgcatccct ttggccgtgc gcgtggcgta gtgtggcgaa tcggggccgg agtttggcgg
     4441 aatgggcgtc atgaacttcg gctcgctcca tttcaggccg tcgggactcg tggcgaactt
     4501 cacccgctgg cccacacggt cctcgatgcc cggtccatcg ctccacatcg cccaaaactg
     4561 gccgtcgtga tgcgcgaggt agttgtgctg gttcacgccg ccgccttttt tatcgagctt
     4621 ggaaggatcg tttcccttcg cgcgcacatc gctgatgacg acatgctcgc cagggacgcg
     4681 aggaagcgcg gcgaagtcga tgtcatgagt gttttccggc atccacgggc cggagagcat
     4741 gatgggagag tctgccgcct ctgtgagtct ggaaacggtg gtcagggtgg cgatgaatag
     4801 caggggacgg atgatcatgg cgtgatgggt gaagcagaca aagaacggaa tcagtgtgtg
     4861 gcgtggcgga tgacctggcc ttgtttgaga aggaggtttt gcaaagcctc agtcggcacc
     4921 tggcgcggct cgacctcgtt ttggatcgcg agatgcgcgg cggctccggc agcttgccca
     4981 agtgccatcc aggttggctc catgcggatg ctggagaagg cgacgtgcgt ggtgctggcg
     5041 gcgacggtga cgatgaggcc atccatcttc tccgggatca tgatgcgata cgggatctcg
     5101 tagggccgcg tgatgtagtc gagcatgccg aggtagcctt cgagcacgag agtgtcgccg
     5161 ggctggcgct tgcggcaggg gaagctgtcg atggggaact cgccgacggc gatgctgtcg
     5221 tcgtgatgct ttggctgctg gcctttgccg gtgatgtcgt gctgcgtgag cgtgtattcg
     5281 ccgatgaggc gacggccttc gcggacatag agctgccagg ggaagtgcgc gttgtcagtg
     5341 aactcgtcct tgggcaggtg catctcattc gccagcgcac gatgagcctc aggcacctcg
     5401 ggatcgtttt gcaggaacca cagcagaccg agcacgaggc tgcgatgccg ctgacggatg
     5461 cgcgcgcgcg tggcttcatc gccctcgatg tagccgcgat tctcctccgg gaagggaaag
     5521 ccgagcggac gcgggttgat gttcacgtcg cacttgttgt tcggaatgtc ggcgacagac
     5581 agggcgcgca cgagcgtgtc gaagtgcttc ggattgtagc cgcgaccagg cttgaagacc
     5641 ttcgggcctg cgagacggcc tgctttgagg tcatcgaagt aaccgaggta atcgcggcga
     5701 tcataccccg gcggcggctc ggtcatgcgt gccgcattgg cgggatcctt ggtcaaacag
     5761 agccggtagg tgtaggcggg cagcgcatca tcgcctttgc ccgtcgttcc aggcaggaat
     5821 tcatgcgcct gatagtcgaa ataaacgacg ccggcatgtg gttcgtcgaa ctcctcgcgt
     5881 gattcgcggc cgagacggaa acgcgcgccc gccgccgcat acaagtcgcc ctcgtaggtg
     5941 gcatccagga agactttggc ttcgagctgc ttttcgtcgt cgttttgtcg cttcacgatg
     6001 gtgatgccgc gcagtttgtt gtctgaaaca tcggctgatt tgagccgcca acccttcagc
     6061 acggtgatgt tcggctcttt ggcgatcatg gcctcgaaga ccttctccgc cacatgcggc
     6121 tccgcgtagt agccgtcctg gcagagcttc gtgtgctcat gctcagggcc atatttcgtg
     6181 atgtagtgct cccgcacacc cgcgacgaac tccttgaaga tgccgccgat catcgcgcgg
     6241 ttctcaatgt cgcttttgcc aagtccgctc gtggtcatgc cgccgatgtg gtcgtggtat
     6301 tcgacgagcg tgacttcacg gccaagtcgt gcagcggaaa tcgccgaagc gataccaccc
     6361 ggcgtgccgc cgtagatgac gatgtcctgg gcgagtgatt gaccgcacag aagcgcaaag
     6421 gtgatgaggc tgaggccaga acgcatgacc gttcatgaac gcttcaatca cgcctggcaa
     6481 tcagagatca tgatttgggg cgcagtgatc aaagatcctc acggaggatc ggagttccca
     6541 tgcctcatcc ccgcagcgca tggatcatgc tcattcacat tgaacctgtt ttgttgcgat
     6601 ctgcgcggtc gccgggcaag cgcagcatgc ggatcgtttt tccttcactt ggccgtctgg
     6661 gcagtggaga cacgaaacat gatgaccttg gcgacacgat gcggcggttc gcggtgaatc
     6721 tgcacggagg ctttcatggt gacttgctga ccagcaagtt tcgcggcgct catgtaatcg
     6781 agtccgtcgg gcttggggtc gaacttcttc ggcgggatga aatactcggt tccgtcgctg
     6841 gcgtggatga tcatggatcc tccgccctgg cctttggccg ggtcgccttt gacttcgact
     6901 ttgccggtga cctcacgttc ttccgtcctc acgacgcctt ccccggcggg catcatcgtt
     6961 tcaaccacgg tgacgacgtg aaatttgccg gatgggtcgg ccacgcattg gccgctcatg
     7021 tgaaagcggc cgacttgaag cccttccatg tccttcatgg ccgccgcacc ttccggcgaa
     7081 cttttggcgc agagcgttct gggatcctcg gggagcaaga gaacggagag cgggccttcg
     7141 ggatgcggca gccaaaactc gtgtttttcg ttcttcacac gggtttttcc ggtgagcttg
     7201 aagggaatcg ttttggcagc ggaggcttcg tctgaagctg aaagtccggt gatgggcatg
     7261 gacagcgcga gcaggatcag gatggttttc atggtgaatg gtcgggggtg aacttgtttt
     7321 aagcgagaag ggggtccagc gggccgcggt gcatgtcctt gtcgagattc ggatcgaggc
     7381 tgccaaagag gccctgtgac gctccggcga ggtggaggag cgtttcgtgc agggcgttga
     7441 tggtgcggtg gcctttgagc ccgtagtcgg gaaactgcag gtagcgtccg gccttgaggc
     7501 gtccgctggc gttgcccagg acgacgaagg gccactcgaa gcagcggctg tgatgggttt
     7561 cggcaccatc gctgagatag acgatgacgg tgttgtcgag catcgttccg ccgccctcgg
     7621 gcatggtttt cagcttgtcc atcgtgcggg cgatgagctg gaagtgaaag gcgcgtatct
     7681 tctgcgactg gtcccagccc gtggtgtcgt cctcgttgta gagcgcgtgg ccgatggggt
     7741 gcttgccggt ctggatgccg agaccggtga aggtgatgtt gaagttcgga aagccgacgc
     7801 cggaggcgat ggtggcgcag ttggtcatgc cgccgatgag cgcggccgtg gccagctcga
     7861 agtgcgcgtc gagccggtcg gtctccacct gcgaggaata cttgtcggtc atgccgggat
     7921 caactttggc aatccgatcc cgcgcctcga cgagccgctg cgaggtggtg ccgagcgttt
     7981 caaaggagga gagataggcg tccatctttt ccttttccaa ggaacccagt gcgctgcggg
     8041 cgcgacggat gtctcccctc atgtgatcga gcaaatgacg acgcgcctgg aaggccgctc
     8101 cggcttctcc tttggcgacg gctccgaaaa gctgggtgta ggccacggag ggatgcagga
     8161 gcgtggccct gctgcgccct cgcccggagg cggagcagtt gaaatccacg accttgtcag
     8221 catcgctgga gatgccgagc acgaggtttt catggatgcc gggatgggcc tggccgatga
     8281 cacagtccac ggtggcggcc tggagattgc gtccgtgatt cgcgtggtag gcaccgagtg
     8341 cgccgtggtc gcttgaatgt cctccggagc acatgcgccc ggagaggccc tggatcactg
     8401 tgaggcggtc ccgataggcc gcgacgggtt tcagcgcggt gggcagctcg gcatcgagca
     8461 gcgaatgctc cgtgaaggca ttgcgctgtt ctctttcgac aaacggaatg ccagccgggc
     8521 agagctgctt tggtgggacg ctgttgccct cgacgacgaa aaggaagcgc agcggcggct
     8581 tgccgccctc cgcgccgagc aacacacggt ccagcagcgg tgcgagcacc ggggcgccgg
     8641 cgcagagcga caggcgattc aggaaccgac gacggttgag catggacgag ttcatggtga
     8701 aagcagtggt tacttggtgg cgagagtttc gggaagccgg cggtaaatga aggagtccga
     8761 ggtgagcagc gaggcggcga gggccctcat gctgccgttg tttttccggt aagcctcatg
     8821 cgcatccatc agcgtcggcg cgtcgtccgg tgtttcgttg cggccgagga aatagcgaaa
     8881 cacatgacgc acaaagacct gctccacata tggggaggtg gaaaggcggg ccagcatttc
     8941 aaaaggatcg gcaaccgggc catcgatctc cggcacaccg atcttgattt cacccgtggt
     9001 cacgacaggc cgctccagtt ccttctggcg ataggctccg aagtcgtcga actgctcaaa
     9061 gggcaggccc agcgggtcca tcttctgatg acagctccag caataggttg cggaggtgac
     9121 ctgtttcatt ttctcccgca gcgtgaggtg aggatcagcg ggcagcttcg catccacacc
     9181 gacgggcgtg tccgggatgc tcccgccgag cagcttcgtc tgcacccaca gccctcgctg
     9241 gatggcctgg tttttttcgt tgtcggagaa ggccagcagc caggccggat gggtgaggat
     9301 gccggagcgc ctgccttgcg gaggctgcac gggctgctcc tgctcccaaa tccactctgg
     9361 agggaaccca tagtcgggat gcaggcttcg gcggaagcgc ttctcgacgg gctcacgctg
     9421 ttcgctcgca tcggaaataa agacgcggtc ttctgtcagc atccggcgca gcacgtcttc
     9481 atccctctct aggatgaaca gcaccatttc gtcggcgcct tcgacacgct cgcgggcgaa
     9541 gatggcattg gcctgccggt ggtctttgaa gacttcaggg gcagcggtgt actcgaagta
     9601 ttcctggaag aagcgcagca gacgcggcga cgtcgtttca aagtcgccca acagccgctc
     9661 cacctcacgg cgcacatcgt cacgcccggc gaggcgtcct tccttggcgg catgctcaag
     9721 cgaaacatcc ggtggcgagt cggtgagcgc gtaggcgatg gcgtgcttca actcctgcgg
     9781 cgcgagcatc acgcgcccat gcgcatccgg ggtgccttgc ccgatttcga agcgatagac
     9841 cgcctccggt ttcatcaaga cggcggccag gagcgcctgc aacgccatgt tcctgcccga
     9901 gtcgcgctgc gtctgttcca ccagcggcat cagttcggca atctccgagt cgtgcggctg
     9961 gcgatggagg cccatctggt attgacggcg gatgatggga tgccagtccg catctgaggg
    10021 ttccttgttt tgcagcaggg cctcgatctc gggggcgacg cgtttcccct gcatctgcag
    10081 cccggcgatg tcccagcaga cattgaaaag cagttccgtg gtggcctcct cgaacaagtg
    10141 ctgccccgca tcatcacgga acgcatgcgg cggggtcatg taggcaaagg cgggatgcag
    10201 gccgtcacca ccctggttgc gtcttggctc caaaaaacgc gcgccggtga gctggttcgc
    10261 acgcggcatg aagaggtgcg ggctcatccg ccacaggcgt gcgggcgtgg ccgcgcggcg
    10321 cacctccggc tcggtgaaga gcacggcatg aggcacgaga tttccgtgtc cgggcagttc
    10381 cgcctctttg gcgcgattca gagccacctc cttcatcaga gggctgcctg cggcaagaaa
    10441 ggccgtgcgc gcttccgaag gcggctgctt ttcgtcctcc ggcggcatct cgcgtgacgt
    10501 gaccacatcc agcacggcct ggagcaggga tggatcggcg gaggcgggat tctcgatgag
    10561 cggtgtgagg ctcaccttgc ccttcatctt cacgggattg tggcagtccg cgcagtaggt
    10621 cttgagaaac gaaggaaccg ccggatcagc cgcccacact gacgatgatg cgaggcacag
    10681 cgagaaaaca aggatgacgc atgagagagt gtggaatgac ttcatggggg tgacattttt
    10741 tgcggttgga tggccagacc atacatgagc tgccgttcag ccgaacgcgc cgtgccgagg
    10801 gtgaactcac cggcgggcgc attggctttc cagacggcaa acttgaagta tgggcgtcct
    10861 tcctcatcga gaatggcgtc cggccaggag gcgatgccga cttgcaacgg tccggcaatg
    10921 actgtctcgc cagtggggct gaacttttcc gccagccatt ccggcagttg atcctgcgcc
    10981 ttgatcagga catagaccac ggcaggttca cgcagtgtca cggtcagctc gtagctccct
    11041 ctgcggcgaa actggtgata ggtgcgaatc aggtcggccc ccagcaaaga tgccggaacc
    11101 tcgtctccgg gacgcggcgc ccagcgtgga ttcggcttgt cggcgaaggc gagcacaccc
    11161 ggagccatgc cccgctgcac gactccatag aatggctcca gctcctcatc gcccagattg
    11221 tcatgcactc gctccacaat gccggtgtaa tgggctgccg ccagtcccgc ctcctgcgcc
    11281 gccaccgcca cctgccgcca gcctcgcagt cctgcctgcg cggaaaagcg cgccgccatc
    11341 ccctcggtca acgtgaccgg aacaccgccc attctcggct gccgcgaatg cacctcgacc
    11401 ttcccggaaa acacggccac gcgcgattca cccgagcggt ccgcctgcac gccaaagcgc
    11461 gtgcccaggt ccacgatctc acctgccgct gtctcgatgg tgaaaccttt cccatgctcg
    11521 ccgacatccg cattcacccc gcccgaatcc agccgcagcc gcatgtcatc gaaaaactca
    11581 ccttccaccg gtgcgctcat ctcaaggcgc gcgcctgacg cgagggcgat ctccagccgg
    11641 cccgtctcaa gtcgaagctc cttcaaccgc aactcacgac ctgcgatcaa ctcaccgtcc
    11701 acacccacga tccgcgcgtt atcagcggag accaatgtcg cagaagcacc tcggggccat
    11761 gggatcgtcc gcagccacag cgtcccggcc atccccagca aagccaccgc cgccgctgcc
    11821 gcgagcggac gccaggcgag ccagcgggtg gaacgccttg cttcatccgc gaccggtgcc
    11881 gtcagcagtt cgcgagtcgc ctctgccgag gcggctttgg cctccagcgc cacatcaagc
    11941 tcgatgtggc ggatgtaaag gtcagccaat gagtcgtctg ctttcaaagc ctgctgaagc
    12001 tgagcggcct ccgcctccgt ggtgagtccg gcgatgtgtc gctggatcag atcgttccaa
    12061 tcggtgctca tgcggcttcc tcccgtgcaa gggtgcgctc cacgcagtcc agcagggcga
    12121 ttcggatgcg gtggagcagt ttgcagagag ccatcggcgt ctgtccgcgc cgggtggcga
    12181 gctcatccat gcgcgtgcct ttggtgtagg cggtgagcac gagttcgcgc tgggaagggg
    12241 gcagcttttg caggcagtgc tccagcgcct cgcggcgcgg catcacctcc gcagcatgca
    12301 gcaccgcacg atccgcaagg cggttcacca gtgtctcatc gaagacatgc cggtcacgct
    12361 tgcgtgactg gagaaagcgc agcacctcca gccgtgccac accgaaggcc cacttcttga
    12421 aatcctgcgc cgtctcgaac ttctgccaca gcaccacgat cacctgctgc atcacttcgt
    12481 ccgtctcatc gcggcaaggc agcatggagc gcacaaacac ctgcaacgcc gcctcatgct
    12541 ccgcgtacag gcggaggaac tgggcgtgct ggggatcgag cggcggctgc atggcgtggg
    12601 gttgccatat agccacagca gccggcctca attgccgaaa aaatttccag cctgcgcttg
    12661 ggaacgcagg ccggtgggca tgctttacgc ccgttagcag caatcggccc acccggcgcg
    12721 tggtgcgggg cctgcgcggc gtgcgttggc ttcctcgctg ccgatgaggc gcttcgtctg
    12781 cgggtcgaac tgaagatcaa cgttcagata ctcggctatg gtgcccaggt tcaacacctg
    12841 cgttagcacg ccggcgacgc tgaagggcga ttcggtggtg ccattgccaa ggcaggcctg
    12901 gatgaagctt tcctgatggc tgtacttcgt cggatgcagg gccatcgcgt ccttgaactc
    12961 gaccatcttg gctcgcggat aaacgagcga gtgacaaact ccttgaagat gccgccgatc
    13021 atcgcgcggt tctcgatgtc gctcttgccc aagccgctcg tggtcatgca ccgatgtggt
    13081 cgtggtattc gacgagcgtg acttcaaggc caagtcgtgc ggcggaaacc gccgtcgcga
    13141 tgccgcccgg cgtgccgccg tagatcacga tttcttgcgc gagcaattgg ccgcaaagga
    13201 acgccagggc gatgagtggg cggccgaaac gcatgaccgt tcatgaacgc gtcaaacacg
    13261 cctcgcaatc agagatcatg atttgaggct cagtgatgga agatcctcac gcaacaccag
    13321 cggcggctca attttccagt gtttgatcgc tcccgccatt gaagccgggg acttcgccag
    13381 ccatcagcgg ccaggcgcca tgatctcgat taaccggttt gtcatcctcg gatacgatca
    13441 cccaccggtc cttctccgca tggaggtcgt agtcgagcgt gccgccgccg ggccacacaa
    13501 agcgccgctt caattgcgac gcgtcgggat tggccgcgat caattgacga agttgcgtct
    13561 cgccatcgcc tttgcccaag aggcagaaaa ggccgagggt atcggtggaa aacacgccga
    13621 tgatctgccc ggcgcgttca tagaggctcc atgcgccgtc cttggatgcc ggctcccatc
    13681 cctcgggaac atgtagtcgc cgctgaattt ctgctgcccc agcgtcgtgc agtggcaggc
    13741 atagccaaag atcaccgcga tctcttttcc atcctccgcg ctcgccttca aaccggcaca
    13801 ctctgatcca ccggacccgc aggattggga aaattgctcc agccgccctt cccatccggc
    13861 gtgcggcggt tcatcgcgaa gccgcagcgg gcatgactgt agtccagatg cgccggcacg
    13921 gacttctcca aggcctcgcc aatcagccgc aggatcgtgt tttcgagcgc ggtggtgtat
    13981 tccgccgtct ccagctcccg ctgcgccaca tcatccgccc catgcagacg gctcgtgcgc
    14041 aactccggcc cgctgtgcgt gtgcgaggca ttgatcgcga ggttcgctgg ctcgatgccg
    14101 tgctccttct tcgctcgctc tgccactgcc agccgcagat ttctcgggac gccgattaaa
    14161 tcgagtgtca ccagcacaaa acgaccgcct tcctgatccg taagcgcaaa gggcttcgcg
    14221 aagagatcca gttccacgct ttccgagggc ttcgtgcgtg ccgcatagcc cgccatccac
    14281 atcgacttct ccggcgtgat cttcgccgat gccgcgcccg ccttccaggc cgccgtctcc
    14341 agcacgggtg ccgcggcgat gacaaacaac gaaaggagaa gcggaagcca gagtttcatg
    14401 gcgtgactgc aagccgacct gcgtctcaat ctttcccatc caaccatgcg ctctctcatt
    14461 ctcctcccgc tcctcaccct cagtgcattc gcccagcagc ccaaacccgc tcccaccaag
    14521 ctcgcgcccg aaatcaccgc gtcactcgaa gcacatcccg gcctcgttta cgcccgttac
    14581 ggccagcggg agatgcagct cgacctctgg aagcccaaaa cggccccgca gccactgccc
    14641 gccatcatct gcatccacgg cggcggctgg tataagggcg agcgctccaa catggcgaac
    14701 ctcgcgcaag ctttggccgc gaaaggcttt gtcgccgcga cgatcagcta tcgcctcagc
    14761 ggcgaggcga agttccccgc cgccattcag gactgcaaag cggccgtgcg cttcctccgc
    14821 gccaacgcat cgaagttcgg catcgacgcc aaggccatcg gcgtcaccgg actttccgca
    14881 ggtggacatc tcgccgcgtt gctcaccacc agcggcggcg tgaaggaact cgaaggtgac
    14941 ggcggccacg ccgatcaatc cagcgccgtg caggccggca tcgccatggg cgcgcagagc
    15001 gatctcgaaa gcgcccgcat cggcgagctc agcagcaagc ctgacgatcc cttctaccgc
    15061 accttcctcg gcgattcaca ggccaaggtc ccgcaaacct acgccctcgc ctcgccacgt
    15121 catcacctcg acaaaaccga cccgcccctg ctcttcatga ccggcgaact cgacgacccc
    15181 agcacccacg ccgacgacgc ccgcgccgat ctcgccaaac tcaccatccc caccggcctc
    15241 gaagtcatcc ctcaagcccc gcactccttc ctcggccagc aaaaggcctt cgacgcctgc
    15301 gtgaatgcgt gcgtggcgtt cttcacgaag cacttgctga aaaactgatc ttcgccattg
    15361 cgcaagacac ggcttcgcag gcaaaatcgg gctaagatga gtccgcgatg ccttcttttt
    15421 ccactcgtgc tgctttgtct gccacacgcc gtccacgccg ccgaggacgc gggcatctcc
    15481 gaactcctca cctggacggc tgaggcacct gactggcagg agcacctcaa acgacgcatg
    15541 agcgagggtt ggaaaccgcc cgctcgtgtg tcgtccacgc ccgaccagcg tgatgacctc
    15601 gccacgctga tcgatcattg gaaatctcat ccctacgggc atcgcgaaga gaaacctgat
    15661 gccgcgaccc gcgagcggct gctcaaagcc tgtgaagaac agccggccgt ctttgagagc
    15721 gtgggctttc gtttcgagcc agcggatgcg ggtgtgcccc agcgcatcca gcagctttac
    15781 cagcgcatgc ccgccaccag cgaggaagaa aagaaagcca aggccaaagc acgcgactgg
    15841 ctcatgtgcg aggccggatt gctccgcccc gaactgaaac aggaggccga agcggcattc
    15901 caacacgcac tcgaacgcac ccgccgcagc tacctcgacg cctttctgcg ccacgaacct
    15961 gccgaaggcg aaaagctcct cctccagcac gcacaggaca ccgaaccggc ctcccggttg
    16021 ctcgcgctcc tgcgtctgtt tgatcaagac gcggctcgtt ggcgctctgg tattcaaaaa
    16081 ctcgtcgaaa gcaacgcagc acccgcttta cgaatcgaag cactgaaagc tctcgctgcc
    16141 aagcgctggc ctggaagcga tgcatggctc ctcggtctct ttaaacacgc tgagttggga
    16201 tacctcagtg aagattatca ttcgagcgaa ccctttgccg agatcatcaa agccgcgccg
    16261 gatgtgtgga ttcctcgtgt gatccaactc gtcggcaaca aagaccgcgc cgtgcatgac
    16321 aacgccgtgc gctgtctggc ccagtttcat ctctcggaaa cacgggaaga cgcattgaag
    16381 ccgctgctgc cttggttgac caatcctgct tgggcgctcg atcccgagca cgatggccgc
    16441 ctgcgcctca tccagtcact cgctcagatc agcctgcctc agagcgttcc ggggctcatc
    16501 tatgtcctgg agcacgattc cggctactgg cgctccgccg ccgcagacgc cctcgcgcat
    16561 tataaagctc gtgaagcggt cccggcgctg cgcaaagctc tgctggtcga aaacgacgcc
    16621 caccaccagc ggagcatcgc ctccgccatg ctgaaattga acgttttttc ctccgaagaa
    16681 ctggtcgagg gcatcgaatc gctcgtggaa caaacgctca cagaggagaa acgctccgcc
    16741 ttgagcgcat tgtcagatcc ctttgcgccc tctgagatgc caaagctccc acggccactt
    16801 ctcgcgcgaa tcgccatcgg ccagtgcacg catgatctgg acgccgatct ccgtgagggc
    16861 gtggcaaaga ctctattgca acgcacgaag gcgctgcgtc ccaaaaacag tgctctagcc
    16921 gacgctttgg agcttcacat ctcggattgg gaaacgcccg ctaatcacga actgcttcgc
    16981 cagcgccttc tcagtggcaa cctcagcgct gaatgggttt ggcggctgct cgaatcggct
    17041 cctacgatcg aaaacgctcc gcccgtctct caagccatca tcgcagttgc tcaccgcgac
    17101 gggaaagccg cggcaaaaat gctcgcagat ccgcatgcgg acgcgcaggc catgctgctg
    17161 gcttgcgcac ggctgaaacg cctcgttttg cccgtgaagg aagtgcagtc cttgctcgcc
    17221 tcgaagcaca tcctcgtgaa acgtgccgcg ttgctctatc tggaggcatt ggacactgcc
    17281 gaggcacggg aactcgtctg gtcacacttc cccggcgagg cacgcatcct cggcgcacgc
    17341 atgggctatg atcccgggca tttctccttt cgcggactcg atcaatggga ggatgttctt
    17401 cggcaacgcg tgctcgctcc aggcggcgcg gatgaaattc atgctctgct cagcgccggt
    17461 tactggggaa acgcaggcca gctctggatc gagaagcgcc ccgacggggc ctggcttgtg
    17521 aacgccgaag accgcggtcg ccagcgggtg cgtaaaatca gccaggacga atacgcggag
    17581 ttgctccgct atttgaagga gcatcaagta gatgaactcc caaccttcga cacaggcaca
    17641 caggacggcc tccagttgga gcatgtccat cttacccgca acggcggcaa gcgtgtcttc
    17701 atgaacaatc cagatagcgg tgccgatgga gcgcccgaag tcgtctatga aaacctcgtc
    17761 accatctatc gcgacctcat tcgcgatact tcacggctca gcgtcgaata ccgaggcgtg
    17821 tccgatgtca caatcgagat tgccgctgaa aaaatgcccg taaaggccgt ctggggacgt
    17881 gccgggcagc tttttgtcca cgcacgcctc cctggcaccc agcgcgaccg ctggtgccag
    17941 ctcgacggaa cacctgcaca gggcgaattc ccgtccatcg tcgatccatg tgacgcgggt
    18001 tctgattcac acctgcaaaa agccgcatgg ctctccaacg gcatccgcgt cggagatcat
    18061 ccacagacca aaaaaagcgg tctatggcat gagaagggtg aatggatctc cgacggagtc
    18121 tttgccgatc ccgtggtgtc tgcggatggg aaagtggtgg tcgtttcacg cgcccgcggc
    18181 aaacattggg gcgagccgaa tgatgtcgtg cgcatcgaac tcggcaccaa gaaggtgctt
    18241 ccgatcgatt tgccttctgc ggacgatttc agcgtcatca ctcgtcttcc cgacaccgga
    18301 aaaatcctcc tcttccgcag ccgcaacgaa cccgttcccg gcatcaagcc caaggaaggt
    18361 ccgcagaagc ccgaatacca cctgctggag gccagcaccg gcgaactcga acgcgtaaaa
    18421 ggtgaattcc agcctttgca cgatgaaact tggcgtccgc tgcaaaacac cgccaagcaa
    18481 ggcatcgtct gggctgcact gccgtccatg gaaaagggcc gccagaccgg cacgctcatc
    18541 gggcgatacg atctctcaag cttccacttt gaaactttca tgaaagtgcc cgtgctgcag
    18601 tttagcagca tggacttttg ggtggaggag tccaaaaaaa gagtgtggat cgtcgtgaac
    18661 aacgacttgt tatcgctacc aatggcagac tgatgaacac acccacctac ttcaccaccc
    18721 acgcctcccc gctcggcgaa atcatcctcg ccgcgacgga gaacggcctt tgcggtctct
    18781 acttcaaaga ccacacaccc gcgccgaagc gtgaaggctg gaaacgcgac aacggtccgc
    18841 gattcgatgc cgcgctcgca tggctggatg cttacttcgc cggtgagaag ccacgcaaga
    18901 tgcctgcgca tgccattgcc agcggcacgc cttttcaacg aagcgtgtgg gaggcgctgc
    18961 gagacattcc acgtggcaaa acgacgacgt atgccgaact cgccgctgcc atcggctcgc
    19021 cgaaggccgt gcgggccgtc ggagccgcgg tgggacgcaa tccgctctcc atcctcattc
    19081 cctgccaccg cgtcatcggc cgcgatggct cgctcaccgg ctacgcgggc ggtttggcaa
    19141 ggaagaagcg gcttttggct ctcgaaggtc tgaaattcga ctgaatgcga cacatggcga
    19201 agtcgcccgc ctatgctcgc atccgcgccg aagtgctgcg tttggtcgcc ttgatcccgg
    19261 agggcaagtt cacgacttat ggcagcatcg cggtacacat gaatgtcgtc gcgcgtcacg
    19321 tcgccacggt gctcagccgg cttacggagg atgaatcgaa ggccctgccc tggcatcgcg
    19381 tcgtgagccg tgacgcgcgc atcagcccga acatggacgc gaagctcgcg gcgaagcaaa
    19441 aacgcctcct gaaggccgaa ggtctgaaaa tcggccgcga cggcttcatt caggatgccg
    19501 gcgcgcactt ccatgtcgtc ggcgtgcggc gaaacattcg ctggagtgcc atggagtgac
    19561 cgtggcgagg ctttccggtc atttttggca cgctgcgggg ccagcgcgtg aatactctct
    19621 gccatgcccg attcgccacc caacatggaa aacctcgtcc tcctgctcac gcagcatcag
    19681 gagccgctgt ttcgctacat cttcagcctc gttccctgcg aggcggacgc gcgtgacatc
    19741 ctgcaggaga cgagcgtggc cttgttccgg aagtttgacc agtatgacgc cacgcggccg
    19801 tttttgccgt gggcttaccg cttcgcgtat ctccaggtgc agaagcaccg cgaaaggtcg
    19861 gcgaggttgc ccctgctgtt cagcgaggat gtgatggacc tcatcgccaa cgagcgcgcg
    19921 cacatcgagc cgcggctcga cgtgcgcctg cgcctgctgg atgtctgcct gggaaaactc
    19981 acaccgcagg acaaggagct ggtcacccgc cgctatgccc tgcgggaggg cgcggaggag
    20041 atgatgcggc gtttcgacat gagccgccgc acgctgttcc gaaacctgga gatgctgcgc
    20101 cagcgcctgc acgaatgcgt gacacgccag ctcgaatcgg agggcctcgc atgagtctcg
    20161 aatcactcat ccacgcccaa ctcgacggtg agatcacgcc cgagcagcat gcgcggctcg
    20221 atgcgctgct gcgggaggac tggcaggcgc gccggcttta tctggaactt gccgatctgc
    20281 acgcgcgcct gctccagcag ccgcagatcg ggaccgggcg gctgccgcag gctgaaaagc
    20341 cgccgggcct gcggcgatgg aggaagctgc tcccggtgct cgcatccgcc gccgtcattg
    20401 ccgtgggatt cctgatgtgg ccgcctgaaa gcacccgctc cgaagccacc ttcagcggcg
    20461 tggccatgct cagccagatg ctggacgccg agtttgcgca aaacacgctg cgcagcggcg
    20521 acacgctcgc tccgggcacg atcaagctca ccaaaggcct cgcgcagatc gagtttttca
    20581 gcggtgcgac ggctttgatc gagggcggcg cggaaatcga ggtcatctcc gcatgggagg
    20641 cgcgatgcgt gagcggtcgt gtgcgcgtgc atgtgccgcc ggcggcgaag ggattcctga
    20701 tgcatgcgcc gggcatgaag ctggaggacc tgggcaccga gttcgcgctg aatgtgaagg
    20761 acggcaccag cgcggtgcat gtcttcgagg gcgaggtcat cgcgcacacg gaccagccgc
    20821 ccgccagcct caaggaaggc atgacactcg gtcagacgga tgccggtttt ctccccatcg
    20881 gcgatgttca cgatctcgtg cggcagcgcc aggagcgccg ccacgctgaa tggcagcagt
    20941 ggtcgcaaga ggcgcgcaaa gacccgcgcc tgatcgccta ctacaccttc aaacactggc
    21001 ccgacgaccg ctgggaccgc ctcgtgaaca atttcaccga gccgcgccgc ccaaaccgcg
    21061 cgggcggggc cgttggcgcg cgctggacgc agggccgctg gccggggaag gaggcgctgg
    21121 agttcaaacg cccgggcgac cgtgtgcgtt tgaatctcga cgacacctac gatgcgctca
    21181 cgctcgcctg ctgggtgaag gtggacagcg tggacaagaa gtacaactcc ctgcttctca
    21241 cggacggcta tgacaatggc gagccgcact ggcagatcta tgaagatggc agcctcatgt
    21301 tctccatcat gtaccggccc gccgatgcgg cgaagaacgc gaaatacaac cagatgtacc
    21361 acagcaagcc cgtcttcacc gccgacagac tcggccgctg gcaccacatc gccgtgacct
    21421 atgacagcca gagcggcgag gcagtgcagt atttcgacgg ttccgaagtc agccgcgagg
    21481 tccacccgct ccaccagccc ggccgcgaca tcacctgcgg tccctgcgaa atcggcaact
    21541 ggggcctgcc caccgaaggc caccagtttc ccatccgcaa tctgaacggc gtgattgacg
    21601 agttcgccgt ctatggcgcg gtgctgggcg cggacgaggt gagggaggtg tttgaggcag
    21661 gcaaggcgga gtgagctccg ggttgaccgc ttcatgatga cgcggctgga gcgggacttc
    21721 gagtgttgcg cgcacggctc cggcattata agaaacccgc catggccagc accatcgccg
    21781 ttctcggcac actcgacact aaaggacccg aacacgccta tgtggcggag ctcatccgcc
    21841 agcgcgggca ccagaccctg ctcatagaca ccggcaccgg cgccgcgcca acagccgcgc
    21901 ccgacatccc gcgcgaggag gtggccgccg caggcggagt ggatctgccc ggcatcctcg
    21961 cccggcggga tcgcggcgag gccgtcaccg ccatgtccgg cgcggccgcc gcattgctgg
    22021 agcgactcgc cgtttcgggc cgcatccagg gcgtcatttc cctcggcggc ggcggcggca
    22081 ccgccatcag caccgccgcc atgcgcgcgc tgcccattgg cttcccgaag ctcatggtct
    22141 ccaccctggc cgccggaaac gtggctccct atgttggcac aaaagacatc gtcatgttgc
    22201 ccagcatcgt ggatgtgaac ggcctcaacc gcctctcccg cgccctgctc gcccgcgccg
    22261 ccggagccat ctgcggcatg gtggaaatgg agccgcccgc cgccgacgac aagccgctcc
    22321 tctgcgcctc catgtttggc aacaccaccg agtgtgtgaa cgccgccagg aaaatcctcg
    22381 aggacgccgg ctacgaagtg ctcgtcttcg ccgccaccgg cacgggcggg cgcatcatgg
    22441 agagcctcat tgaaagcggc atgatctccg gcgtgctcga catcaccacc accgagtggg
    22501 ccgacgaact catcggtggc gtgctcaacg ccggccccac ccgcctggag gcagcgggca
    22561 aagccggcgt gcccgccatc gtcactcccg gctgcctcga catggtgaac ttcggcgagc
    22621 ccgccaccgt gccggagaaa ttcaaagacc gcctctttta ccagcacaac ccccaggtca
    22681 ccctcatgcg caccagcgcc gaagagtgcg cccagctcgg ccgcatcctc gccggaaaaa
    22741 tcaacgccta caccgcgccc gtcaccgtcc tcatcccgcg caaagccatc agcgtcatca
    22801 gcgcccccgg ccagcccttc cacgaccccg aggccgacga agccctcttc tccagcctca
    22861 aatcccatct ccgcgtggac atccccgtca tcgaaatgga ctgcgagatc aacgcccccg
    22921 aattcgccgc cgcctgcgcc cgcaccctgc tgcaacaaat gcagggctga agctcgaaat
    22981 tcgaagctcg aagtgacatt cgattttcga acttcgaatt tccccattcc gcaatccgca
    23041 atccaaaatc cgcaatccaa atgtcccccg gccagcttct cctcaccggc gtccccggac
    23101 ccgaactcga ccccgacacc gccgcccgct tcaaaaagct ccagcccggc ggcttcatcc
    23161 tcttcgggcg caacatcaaa agccccgggc aattgcgcaa aatcgtggac gacctgcgcg
    23221 acctctccga caccgagccc ttcatcacca ttgaccagga gggcggccgc gtctcccgcc
    23281 tccgcctcat cggccaggag ccgcccaacg cccagcagct ccgcgacaag ggcgaccccg
    23341 ccctcatcaa gcagcacggc cgcctcaccg gccagctcct ccgccttttc ggcttcaatc
    23401 tcgacctctg ccccgtcctc gacatctcct acgacgacac cgccgacaac tcactcaaag
    23461 gccgctgctg gggcaaagac ccgcagcagg tcatcaccaa cgccggcatc ttcaacagcg
    23521 ccctgcgcaa ggaaggcatc ctcacctgcg gcaagcactt cccaggttac gggcccgccg
    23581 agtgcgaccc ccacgaattc ctccccgtca tcaccaaaag ccgcgccgac atggaaaaga
    23641 gcgagttgct gccttattcc gccctgctgc ctgagctgga cagcgtcatg acctgccaca
    23701 gcaactacac cgcctacgac cccggccgcg gacgctggcc cgccagcctc agccgcaaca
    23761 tctgcacccg cctcctgcgc gaccagctcg ggtttgaagg cctggccatg accgatgacc
    23821 tcgacatggg cgccattttg aatgaagtca ccttcgagca ggccatccag gaagccgtcc
    23881 gcgccggcaa cgacctcgtc atgatctgcc accgcgtcga aatggtcgag caggcccgcc
    23941 agcacctcga aggcgtcgag cgccccgtcc tgcacgacgc cctgctccgc atcgagaaaa
    24001 ccaaaaagcg cctcgtcaag ccggatgcct tcagcctcga ccgctttcag cagatcaata
    24061 gcggcatctg ggacctccgc gtcgccaccc tcggcgagga gcgcgctcaa atcctcagcg
    24121 tcgaggacgg caaacgctcg ccagtggaac tgtattgagg catccacgcc cgcggcgccg
    24181 ggctttccag tgacgatggc tgggcggtgg ggttagtcgc gaagagtgcg ggggcggtcg
    24241 gtgtagaaaa tcatgggcag ggctttgttt tcctccttgc cgacgatgat ggcgatgagt
    24301 ggcatgggca ctttgaactc gaagtcgggg cggggaggga actcgcggtc gaaggcgagg
    24361 acgatgccgc ggaagacgag gtcgggccat tcgcgccagg ggccgctgaa gacttcgtgc
    24421 aggtgccggg cgcgggcgct gaggggggtg acgatgaatc cggcgacctt ggcgcctgcg
    24481 gcttcggctt tggccttcat ggctgggtag tcatcgcgcc gggcggggat ccagagggcg
    24541 ggcagatcgg cataccaggc gacaaaccag ggtgcgtcag cgaagacgac ctcgcccggc
    24601 tccaacaggc gttggacgat ctgcacgcgg tcgggcgcgt agggcggcca gtgggggaac
    24661 atgcgggcgc gcagggtgag gccgagcttg agctgcggtg gcagggtgac gagcatgggc
    24721 acggcggtga ggccgatggc gagcgcggca aagccgagcc gccgccagaa tgacgaggcg
    24781 gcattggcca ggggataaag ccgcgcccag agcacggcca gcatggccgc gccgaagatg
    24841 gtgagggcgg ggacgagcac agggatgagg ttttgctcgt cctcaaaccc gccgggcagg
    24901 ccgagcagga tgctgccggt gaggatgccc aggccggtga tggccagggc gcggcaaagg
    24961 gaggcggcgg cgggattcct gaaccggtgc aggagggcgg gaaaaaagaa cagcgccggc
    25021 aggctgccgc cgagatggct gaaaatgccg cctgcgagct ggaggctgtt ttcggcggcc
    25081 ttgcgcagga gggcggccat gtccacaggc ggggcggtga ggctgaaggc gcggtgcagc
    25141 agatcagggt cgccgatcag gaggtgtgcc tgcaaaaggg acttcacgcc gccgagcggg
    25201 tcgccacagc gctgaaacat ccagaagccc caggcaccca gggccagcaa aggcggcgtc
    25261 acgacgagca gggcggcggt gaagcgcctg gcggggaagg tgagtgccag ggaaagcccg
    25321 agcccgagca ccagccaggc cgccatccag tgggtgagcg ccatgagtgc ggccagcagc
    25381 cccatgccga gcagtgcggg ccaggcgcgc gccccttcgg cggcggcgct ggcggcggcg
    25441 gcatggaggc ggaggctgag cgcggccatg ggcaggagca gggcggcggg gctgccgctg
    25501 acggccaggt gccagagcgg ctggcaaaaa agcagggcca gaacggaaac ggcggcgatt
    25561 ttttcatcga aaagccgccg ggcggcgccg tgcgtccaca gcagggtgag caggagggcg
    25621 gtgacgccga tgcaggcgat ggcgcggtcc agcaagtaga tggggccggt cttcaagggg
    25681 cggtaggcct gccacttttc cagcagttta aagacgggag ccagcagcag aggctgcacg
    25741 ggcggctggc tgatgtcggg catcgtccgc ggggacgcat ccgcgccgtg ggcgatcagc
    25801 cgcgcccagt cctgcgggcg gatgaccagg gtctgccagc cgtgaccgcg cgccagttcg
    25861 cgggcaagct gcgcctgctc catgccctgg gcgctgcgca gcccctggaa ggtgacaaaa
    25921 acatgcgtca gcgccatgcc cgcggccagg gcgtaaacgg ccagccggag caggggaggg
    25981 ctggcggtgg cgggcggttg catggatggc gcggaggatg ccacgcgcgc cgcgcggggg
    26041 caagcgggca agctgcctgg cgcggtgctt tcccggaatg gcgtaagcct ggagcgcggc
    26101 ctttcggcgg aacgcgggca gtccgcgcgg cgactggcgg accagccgcc gcgcacggga
    26161 gatccaacgc ctcactggta ggaggactcg acgcggcggg ccacgtcgcg gtagccgtag
    26221 tcggcgttgt agatttcctt gagggcttcg agatactgct cctggcggcc cattttttcg
    26281 ttcacgaggg ccagctcgta gaggatctcc tttttggtgt tgtccatgat ggccagctcc
    26341 ttggaggcct cggcgagggc gctctgggcg aggtcgttca tgttcttgcg ctcgaagcag
    26401 cggccgagca ggagcatggc cttgatgcgg atgttggggt tgctcttggc ctgctggagc
    26461 tcggggatgg cctcggtgtg catgccggcg ttgaagtagg cctggccgag gtcaaagcgc
    26521 acctgcttgt cggtggggtt gcgctcgacg cgggcgcggg cctcgttgat cgcggtctgg
    26581 gcgcgctcca tgcggatctg ggcgagctgg gcgcgctttt cctcgatgtc cggagcgccg
    26641 gggttggcct cgatgtcggc ctccatctgt gcgatgtgga tgtcgcgcac tttgtcgcgg
    26701 atgagctcgg tgcggcgctg gagggccacg tctccggggt tgagggtgag ggcgtactcg
    26761 tagaacatga gcgcgttgtc ggactggtcg agctcgtcgt agagctgggc catggccttg
    26821 acggtgttga tgtttgtctg gtcctggttg tattcgtcaa cgacgcgggc cagcagggac
    26881 tcggtctgct cgcgggtgcg gccctgcttg ttgagcagtt cgaggatgcc ggcctcctcg
    26941 gcgtttttca tggccttgcg gaagtctccc tcctggtccc agccctggcg caggatggag
    27001 ctgcgcgcgg cggcgtcctt ctcgcctttg acggcggtga tgtcgcgcgg gtccactttg
    27061 acgatggctc gatagacctc gctggccttt tccggctggt cgtgcgccat gtagtgctgg
    27121 gccagcgtgt gcatgttctt cgtgttctcc ggctggccca tgcggatggt ctccagcgcg
    27181 aaggcggcga ggtcggggaa ctggaccttc atggccaggt cgtagagctg ctggttggct
    27241 ccgggattga aagggtcttt ctggaagaca ttctcctcca ggtcggcgat ggcctcccag
    27301 gggtctttct tcgtgccttt gaagaggccg ccgccgaagc tgaacttgcg cccgccgccg
    27361 gccacctccc cctccgcgcg gcgcagggcc atgcgcccgt cgaggaagcc gggcagctcc
    27421 tgcaccacgg gcaggatgag gccgacggcg tattcccagt ttttctgttc gatggccagc
    27481 aggccctttt tccagaggtt ctgctgcttg ggggtgagtt cgttctcttt gacgatcatg
    27541 cgggtttatt tgctgggcgt tgatttgggt gtcgggggct cctgcgcggg agcggcgctt
    27601 ttttccgggg agacgagctg catcagagcc tgccactcct cgcgcacgcc ctggctgaag
    27661 cccttcatgg tgaatccgtg ctcgaagagc accatccagc agaacgtggc ggccaggaaa
    27721 gcgaggagcc agaggatttt cgagagcagg cgcatatccg gggaaaaaac cgaaattaca
    27781 gataaacagc cgcgcgcgtc cagcacggag tttttttgtt ttgaaaaaca cgcgcgcttg
    27841 ccaggtccgg ccctcccgtt tcagatgccg gcatgacacc gctccaccac ctccgcgcag
    27901 gcatcatcgg caccggcttc atcgcccccg tccacatcga ggcgctcagg cgcctcggcg
    27961 tccaggtcac cgccgtttgc ggctcggaaa aaagcgccag cgccgtcgcc tccctgtggg
    28021 gcatcccgca agtcttcggc ggctacgatc accaggcgct gcatcgcagc cccgacgtgg
    28081 acgtcgtcca catcacctcg ccgaacaaag cccatgtcga acagagcctc gacgccctct
    28141 cccagggcaa gcacgtcatt tgtgaaaagc ccctcggcat gacctccgcc gagaccgcgc
    28201 gcgtcgtcga ggccgtcaaa aagggcggac gcaaaggccc cgtcttcgcc gtcaactacg
    28261 tctgccgctt cttccccgcc gtgctccaga tgcgcgccat ggtcgcgcgc ggagacctcg
    28321 ggcggatcat ccacgtccag ggccgcttct tccaggactg gctgctccac gacaccgact
    28381 acaactggcg cctgctcgcc tccgagggtg gcaaactgcg cgccgtgggg gacatcggca
    28441 cccactggat ggacgccgtc agcttcgtcc tcggcgccag agccacccgc gtcttcgccc
    28501 acatcgagac cttccacaaa atccgcctgc gcccgaagag cgaagtccag accttctcca
    28561 aaaccgccgc cgccgccgtg aagctccagc cctacaaagt ggacaccgag gactttggca
    28621 gcgtgctgct gaagttcggc aaggcgaagc acggctactg cgacggcgtc cacgccaacg
    28681 ccctcatctc ccaggtcgcc gccggctcga agtgcagcct cggcctctcc atccacggca
    28741 ccctgggcag cgtgcagtgg gatctccagc agcccaatga aatcctcctc ggcaggcgcg
    28801 accagcccaa ccaaatcctc cagcgcggca cccccggctt cctcgaggac gtcgccaact
    28861 tcaccgacta ccccggcggc catcccgagg gtttcagcga cgcgcacaag atgcactacc
    28921 gcgccgttta cgagcacatc gccagcggca aaaaaacgcc cgccctcttc gccaccgccg
    28981 aggacggcca ccacgaggtc aggctctgcg aagccatcct ccacagcagc cgcgcccgca
    29041 cctgggtgaa agtgtgagcc gcgcgcgccg gtatgttttt caccatgccc ggccccaacc
    29101 tcatccagca agaaaacgca cgccccggct ccagcgactg gcagctcacc cgcgtgcggc
    29161 tcgacaaaag cgccggcttc cgctcccccg ccatcgaagg ctacgcctca cgccagtccg
    29221 tcaaagccgg cgacaccctc gacattcacg tcagcacccg gcccgccgcc cgcttcaaga
    29281 tcgagatctt ccgcaccggc tactaccagg gcaaaggcgc gcgcctcatg acccagctcg
    29341 gccccttcga gggcagagag cagcccgtgc ccgacatgca gcccggcacc cagctcatgg
    29401 agtgcgtctg ggaaaagacc gccaccctcg ccatcccccc cgactggctc agcggcgtct
    29461 atctcggacg cctcaccacc ctccccgact ccgccgacga gccttattgg caaagctaca
    29521 tcgtcttcat cgtcaccgac gaccgcccgg cggacatcct tctccagtgc agcgacaaca
    29581 cctggcaggg ctacaaccgc tggcccgacg ccacctccct ctacatccat cccaaaggcg
    29641 ggcagggacc gtggccgcac gtcagcttcg accgccccta tgccaaatac gcccagattt
    29701 atgaaaaccc ccagtccatc ggcagcggcg agtggctctg cttcgagttc cccatggcct
    29761 actggctcga acagcacggc tacgatgtca cctactgcgg caacagcgac atgatcaccc
    29821 ccgatcgtgg cttgaaatgc aaagccttca tcagcatcgg ccacgacgaa tactgggaca
    29881 tccgccaata cgaaagcgtc gtccagatgc gcgactccgg cgtgaatctg ctcttcctct
    29941 ccggcaacag cgtctgctgg gtcaccccct tctcacccaa cgccgccggc atcccccgcc
    30001 gccgcatctt ccgcagcggc ccctacggcg gcgactacga atacgccgtc caccgcgagc
    30061 agaaccacgg ccccttcccc caccgcggcc ccgacgaagg ctacctcatg ggcgcccgca
    30121 acgtccgccc cgtcaacggc ggcggcgact ggcattgtgt gaagccggag cactggatct
    30181 tcgaaggcac cggcatgaag gccggcgact ccgtgcccgg cctcatcggc tgggaatacc
    30241 acggcgaccc ccccgccgac ctccccggcc tcgaagtcat cgccgccggc accgcctggc
    30301 agggcgggcg cgtgccccag cagtggaccg ccacccttta tcccggcccc aagggcaact
    30361 acgtcttcaa cgccgccacc atctggtggg gcctcggcat gagcaagccg cccggcacca
    30421 tgcccgtctg gagccactac tcccgccccc acggccccga cccccgcgtc cagaaaatca
    30481 ccgccaacct cctccagcgc gccctccgct gaagcccgcc gcccgtagcc gccgccgcaa
    30541 ggcggtgggc atcagcaggt tcatggctaa actcatgccc catcaacctc ctccagcgcg
    30601 ccctccgctg aagcccgccg cccgtagccg ccgccgca
//
LOCUS       DAOMYJ010000036        30416 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Prosthecobacter sp. isolate AMR_MDS_5629
            NODE_540_length_30416_cov_22.900695, whole genome shotgun sequence.
ACCESSION   DAOMYJ010000036 DAOMYJ010000000
VERSION     DAOMYJ010000036.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867850
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prosthecobacter sp. (wastewater metagenome)
  ORGANISM  Prosthecobacter sp.
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Verrucomicrobiaceae; Prosthecobacter.
REFERENCE   1  (bases 1 to 30416)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 30416)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.30
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:34:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,742
            CDSs (total)                      :: 4,684
            Genes (coding)                    :: 4,676
            CDSs (with protein)               :: 4,676
            Genes (RNA)                       :: 58
            tRNAs                             :: 55
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30416
                     /organism="Prosthecobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_540_length_30416_cov_22.900695"
                     /isolate="AMR_MDS_5629"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1965333"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..560
                     /locus_tag="PLP58_05870"
     CDS             <1..560
                     /locus_tag="PLP58_05870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009960418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="TCP-1/cpn60 chaperonin family protein"
                     /protein_id="HRJ08075.1"
                     /translation="TSDYDREKLQERLAKLAGGVAVINVGAATETEMKEKKARVEDAL
                     HATRAAVEEGIVPGGGVALIRAQAAIGDLGLVGDEKTGAEIVARAIEAPLRQLAANAG
                     VEGALIVAEVKKGKGNSGYNVATAKYEDLIKAGVVDPAKVTRSALQNAASISGLLLTT
                     ECLIADIPKEEKADAGHSHDHGGMM"
     gene            complement(796..1644)
                     /locus_tag="PLP58_05875"
     CDS             complement(796..1644)
                     /locus_tag="PLP58_05875"
                     /inference="COORDINATES: protein motif:HMM:NF024777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ08076.1"
                     /translation="MKMLRLLLLSTLLPLCAARAQAAEALKQALTFHASFDTGFDADF
                     SKGSKTCVTKRGKELVPVSAGDEVRIAPNAGRFGGALHFLKKGATRPQFAGAGILGYN
                     NQSWSVSVSAWLRLTPDEDLEPGYCDPVQIIGDDTKKGFIFLEWSKDETPRFFRYAVR
                     PLHEIWNPDNLQWDQIPAEKRPMVQLANAPFSRERWTHVVFTLDKANEKGAKPAGVLH
                     MDGERKGAIEGWDLTLGWDPAQVALVCGAAYVGHLDDLAVFNRALGDDEVKALFNLDE
                     GVRSLY"
     gene            1708..2064
                     /locus_tag="PLP58_05880"
     CDS             1708..2064
                     /locus_tag="PLP58_05880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018970747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HRJ08077.1"
                     /translation="MTLDTLRTLCLSMPQAEETQPFGPDVLVYKVAGKMFALTVPEDF
                     PPRVNLKCDPERALDLRDEHESILPGYHMNKRHWNTVVLDGSVPAALVREMVRHSYDL
                     VVAGLSAKARRALQTG"
     gene            2113..2892
                     /locus_tag="PLP58_05885"
     CDS             2113..2892
                     /locus_tag="PLP58_05885"
                     /inference="COORDINATES: protein motif:HMM:NF012707.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HRJ08078.1"
                     /translation="MHLLLRALLVFALAFLACCSRRDALVVGMDATYPPFEYVDKNGS
                     ITGVSTAIGEEAARVLGRPVVFQNMPFDGLIPALQTGRIDLIISSLTANDQRRQSIDF
                     SEPYVRTGLSILVAKDSPVQSADDLRAPGRKLAVRIATTGEQWCRQELPQARLTALDT
                     DAACVLEVVNGKVDAWVYDQVSIMNYHMQHPERTRALLTPLREESWAVGVKKGRPDLM
                     AAVNEALARMKTDGRFTELAEQYLAKERALMKAQGLPFVFE"
     gene            2955..3881
                     /locus_tag="PLP58_05890"
     CDS             2955..3881
                     /locus_tag="PLP58_05890"
                     /inference="COORDINATES: protein motif:HMM:NF012607.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxyacid dehydrogenase"
                     /protein_id="HRJ08079.1"
                     /translation="MESLLLLAPLPEFLLSPLKAVYACHDGTARLDDVVAEHGAEVRG
                     IVMAGGSAAPAPLLERLPALEILAVFGVGYDGVPVDYCRARGLRVTNTPEVLTDDVAD
                     IATALVLMTARRLAEAERFIRAGEWSGGAFPLARALRGKVAGIFGLGRIGAAIARRLE
                     AHGMRIAYHGRRRQAVEHDYFPTLRELAAAADFLVVSCPGGAETRHAVDAEVLAALGP
                     EGTLINIARGSVVDEAALVSALEAGTIRAAGLDVFENEPHVPEALLASPRTVLLPHVG
                     SGTHETRMAMAALVVENLAAHFAGRPLPTPVC"
     gene            3925..4515
                     /locus_tag="PLP58_05895"
     CDS             3925..4515
                     /locus_tag="PLP58_05895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ08080.1"
                     /translation="MKFLLASLLAALMSFAWGLLSREVIGWRATGEHAFLNESAVAEA
                     VAKGAKNGRGIYLLPYPSKAPAFGRGEQAKMDEAHAKALKRGPYMRAVVRPGARERNF
                     GVDLAWSFARSLACALVLAGLLSPALLNYAGRLAFCGGAGAFAGLAFMLPEMIHYELP
                     ARAVVVAVADGFVEWLLAGVVLACFVGREPTARDVV"
     gene            4592..10600
                     /locus_tag="PLP58_05900"
     CDS             4592..10600
                     /locus_tag="PLP58_05900"
                     /inference="COORDINATES: protein motif:HMM:NF017369.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007154 - cell communication [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Calx-beta domain-containing protein"
                     /protein_id="HRJ08081.1"
                     /translation="MPKFQTRPLVKLAFSLLPALFLGLLLWWLARQEKTTAPDQPGVV
                     QTSATAPSPLVATAPGIGARMDEKDVFTKFEEWAANFAGGEASAGVELARERRAALKT
                     LIISDPKAALDRALPWTLRRRMPHEVADLLEERVSARGEFSVMAGLPLPGTDARPFYQ
                     RSFTVNGGRSYDAYVYGRREHVGTKYDFPADGIAVDEALALRERPARSPEPEEPAQPG
                     EVVRVAQDGHEVAANGGVPLVAGGTSYRVCCMAHAGAVEQGWVEAEQSTGPWMDPDVA
                     PSVWTEGPKTALVIRVDFPNLPGTPRNTNNQELTVDYVTTEMNGDVANYMRDVSYRKT
                     SYTTATTDVTAVLRMPSNSTFYSGSGGANALRVDALSAATAAGFNVNSYDRQLVAFSS
                     IPFPWAGLGQINGPFTWYNGYLQDLVIIHELGHNLGLPHASLWRGTGGNPVSTTGTLQ
                     SYGDPFDCMGNNFNGPVPRMWFNAWYLNRMDWMPNAAVTTVTVPGTYRVYRYDDPDAP
                     LNRRLALRVQKDATRSYWLAYRRQFAGLASGLADISAGAQVSWGFGNTYNDSHLIDCD
                     TPTTNAQDASLNVGSTLIDLDAGISFTVQAAGGTGVDAYLDISVVQTGRIYPLQTQYD
                     VDEAAGTVTVQLARTGTPSQGASVTVTTQNGTAVAPGDYSSVSTTVTWSGTDITPKPV
                     VIPVVADAVKESAESFFVNFEPAPGSGPEVIVVGSPVTVTIREPGMRDNTFAHEEFEE
                     PGSVKNLLAAPDGRVIFTGEATELDDEDVDGLGRLENDGALDATFIQGGGMLPVAGRV
                     VARQPDGKLLFGGNFVVLRDAPANRLARLFADGFSDNTFNIGSGPNEEVRALAVQADG
                     RILVGGRFSVWNGAPRLGLARLLPNGALDTGFMAAALPHISVMEVEALVLQPDGKVLV
                     GGLIHTESTGQIFTGGLSSGVLRLHPDGTLDTSFDIGAGAHLEGNTGTPARVSTLARQ
                     GDGKVVVGGAFTGFRGVTARRIARLNTDGTLDIGFQSAMGVNGPNNPVRSVAVQGDGR
                     ILLAGEFSQVAGVIRHQLARLLPTGAFDNGFDAAVPLGAANFGNHVIQQPDGRILLAT
                     EAAGTDSIWRLFSGQLGRAGVIQFSAGNHQANEGGGGQIEVARVGGSLGAVSVNYATL
                     PGTAGTGDFTPVSGTLDWVDGDTTSKFITVQVSEDAIPEPVEFFTVQLGVPSGGAFLG
                     ETALASVAVLDPGASDFARVRFTTDSSHAFEFATTPVLVTVEMTPAKAEAVAVPFTIS
                     GTATPGIIGDYEITPAVSPLVFAPGETTKTFSITPKQDKITEIGDETVILDLGFPQGE
                     ALLQAPFRHTFTLVDDDTAPDVSSDSNNQIVQAGQLAGPFEGQVSGSAPLSLEWQLNG
                     KKVPGAGQPHLLIPAARVQDGGEYRLVASNKITKNIRGQASHLVVVDTRVRTVVVPLG
                     GKAVFLAEAGGNALGFEWRKVDGPFPFTRASTRFDRKLTLSKVELDDSGVYICRVSNS
                     RAATAGGDLFMDAGPVVLKVVDTAPLIGLVENELLPPATVGGTYTYEVPVDPEVRKAP
                     IKWTAAGLPSGLKLDAVTGIISGRPQAPDKNGKPYDVILTAANNGGKGVRKVRLSVAQ
                     MPPGLAGSYTARLERHTAINGGLGGRLDFTVTPTGSLSGVLFLGGTALKFTGVLEVDV
                     NGVALPKVEELRIARPGKPVAPPPLVVGFSIEDNLVKAGTISDGAQTLDFTGWRNVWS
                     FAANRADKFAGPHNFVLEMPSSIPDVPRGYSYGTAQVSASGVATYTGMTADREKITGS
                     ALLGPDGKLVFHQALYKNTPQGSLNGEMLIEEGALADDPADNLLSGSLSWVRPAMSKP
                     RLYAAGFGPVDVAVLGGRYQAPALPLGLAGPGRVRLVFDHAGLPASREPGITLDVDAK
                     GKLIPPLKEDNRAGTTLKITAASGLFSGKFSLEDPNPVPPPAFMKRPTTFQGIIVPVG
                     GELRGYGWFILQQLPDDMEGTTVKTSPFLSGEVIFEQVPVVTTP"
     gene            complement(10604..11689)
                     /locus_tag="PLP58_05905"
     CDS             complement(10604..11689)
                     /locus_tag="PLP58_05905"
                     /inference="COORDINATES: protein motif:HMM:NF014069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HRJ08082.1"
                     /translation="MDEHDFELLTLAQLRQNAGPAPLPCRVHAQVDLHQEKKTSTGSP
                     YLEIKLADAGGSLVWRVFDNNPALAEARELRRGQFIQLSGNWVDGGKYGPEPRHPRLR
                     PLTDDEARDLLKGDRALAERQRQDYAEIEAFMDSLADPRLRGLARLFLEKHGARFHRT
                     AAARENHHARRGGLVEHVAQMMRAAAAIAGVYPMLNRDLLLAGVLFHDCGKLWENCYP
                     ESGFAMPYQLHGEMIGHIPLGMELVNKLWRELMDLPEAAEWTALEPASDLARLHLLHL
                     IAAHHGELQYGSPVLPKTPEAVALHYVDNLDAKLEMLRRGYETSTEIGPGILERFRPW
                     ATHILEPLAAHTPPAPAPSEEAREETA"
     gene            11826..12554
                     /locus_tag="PLP58_05910"
     CDS             11826..12554
                     /locus_tag="PLP58_05910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ08083.1"
                     /translation="MDTIPSYRQRLPHWLNLWMPLFILVYPFILSVPSLNWEKDLYRE
                     YGLIENLTVAFLAAAIVLFLRSLRRADGLVHRAWLVLLAAGAFVFLGEEISWGQHYFN
                     WVTPEEWKEMNRQGETNLHNIKGGVEYFFTKVAREGLSLATIIGGILVPLHLRRKKAD
                     FTPGTLWFWVWPSLGGALTALLSATIRLPNSIAHQFGKVDCLPPFLGEDIGEMKECYL
                     ALFILLYALTQFRITRRLPAAGTS"
     gene            12562..13323
                     /locus_tag="PLP58_05915"
     CDS             12562..13323
                     /locus_tag="PLP58_05915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007417985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TSUP family transporter"
                     /protein_id="HRJ08084.1"
                     /translation="MMTLTTAVLLFLAGLSAGFIDAIAGGGGLISVPALLWAGLPPQM
                     ALGTNKMQSTWGTLLAVVRYSRAGLVDWRQMRAGIAVTFTFALLGTWVVTRVSNEALK
                     QIVPWLLLGIAIYTLLSPRLGSRPARARMEPLPFACLIGALIGFYDGFFGPGTGTFWT
                     VACLSLLGLELTRATAYTKVVNLASNIASLLVFVIAMRIRYDVAAVMIAGQLIGARLG
                     SGMVIRHGAPFIRVVFILVVLAMVAKLLLDQWTAG"
     gene            complement(13380..13643)
                     /gene="rpmB"
                     /locus_tag="PLP58_05920"
     CDS             complement(13380..13643)
                     /gene="rpmB"
                     /locus_tag="PLP58_05920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009958835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HRJ08085.1"
                     /translation="MAKVCQITGVGVTRGHHIHRSGKAKKEGGIGKHITKRVKRVIYP
                     NLRQKRIWVPELGKYVTVKLTARALKTLNKNGALKTLKAAGLV"
     gene            complement(13734..16049)
                     /locus_tag="PLP58_05925"
     CDS             complement(13734..16049)
                     /locus_tag="PLP58_05925"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HRJ08086.1"
                     /translation="MNGFDRIRILIVENEGLVGCDMAGCLNDWGCRVVGTCASGEEAL
                     RLNRELRPDLVLMDVHLDGTMDGIETARRLREQGPVAVVYATACADLDTVNRARDTDP
                     YGYLLKPFNHDDMRLTVDVAVTRLQKERERHLREQRCFEAFQSLADGVIAADLAGVIT
                     FMNAAAARVTGWERSQAVSRSLHEVFRIYQASGEPAMVELTGGGANAERTVWLTTHDG
                     SRVPIQDRSAPLRDEAGQLTGVVILFRSLPVTEAEKSDAAHAAAPDATAQAPLVDVVE
                     SISDPLIALDGRWRLIYANASAQRLFERGPDKLMGVNFWDLLPMATREAHHEALAHAL
                     LHRESVSREVYFEEKQTWLELRGHPFAEGLLVLMQDITTRREEADRRHRLDRLESLGL
                     LARGFAHEFNNLLTVLLGNLSLAEMRLRNVIQLPELHTAKQATLQAQGLVQQLLTFAR
                     GGAPIKRPVQMADLIEQFFMHHTRATGIEYRLDIHPRLPTMAVDPAQIRRLIGNLVRN
                     AEQAQPEGGVITVRCHPMDPREMFPHEILSEAPVQAPGGVVVEVHDQGAGIPSEVLPH
                     VFEPYFSTRKSENATGLGLTVCESIAKAHGGSLSVSSEPGKGAKVRLYLPLDDEGDET
                     GSLGIGGQLFDSAPQTATQRILVLEDDPLVRSLIVRNLGGQGYEVAESVEGGETVRLY
                     QEAAQAGRPYDLVILDLSIPNGMGGVRAMEKLRQLDPGVLAIVSSGYSDDPVMARPAA
                     YGFAAVLPKPYEPADMLRLVKTVLAQRAVGK"
     gene            complement(16079..18892)
                     /locus_tag="PLP58_05930"
     CDS             complement(16079..18892)
                     /locus_tag="PLP58_05930"
                     /inference="COORDINATES: protein motif:HMM:NF013179.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HRJ08087.1"
                     /translation="MDLEMETVLGVLVENAPVAMAMFDREMRYVLANRAWIEEFSLQE
                     VQPLIGRSQYEIFPAMHPGWREVYERGLSGHVVRSEHDEIGGEDGGRLVYRWEVRPWR
                     RNKDASVGGLMVTCEKFAAMPSGVSALPPELPMDSRSEQVRHAPDIEMPPSALPLVLV
                     DENCVIHQANTAAVELCLARGLREGASLFAEMFTSGRDIAAFQSLWQKALDKLESGSS
                     GAMLPVPGAHERMSGESELPERWLLSPSGKSGDRMIFLAVGMPRGQGHDPVPRPAAMP
                     AAPDLPAIASAVASIRQPSQQTPADPSANLEVRRLQDDLARTRQEMRTLREAEHLFAQ
                     KETRGRQYLDLLPCGVLVLDESGAPVFQNEPLSRLLGRPVRKDETVENWLAAACPGDE
                     GKQETSTIWREDVWRRQLTRIFSLVTADGLLKELEFQPCALPGGGLLVCIQDATEHCR
                     REEQLRASEAKFRAMFHDAPVAVILTDKTGSVFEANPRAEAMLGRSKSELRRLPPGGW
                     MDAPDIERRKETLRQLQASGDRSASLDIRLVRAGADPMPCAMTLAQVLDADGLPHNTV
                     HFLQPAGSAQTGQKLEGGTSTTSLKTKAPSAIPPASGGPGVFLLRTNVNGRIQDCSPR
                     GLELLGLEAGQAKGRALHLHFRPSDATGFYSELAANSQDERTFECLQAGGERASCRLL
                     ARACGGGGFDVELFEAGGDDLRQLSDKAPPNRSTADSPALKPWPAADLSREKLLLSET
                     HHRIKNHLQIISSLLNLESNTVQDSAARGALRSSQNRVRAIAELHQHLYQLALGSNSS
                     FADFAAGLVQRLRDCFDVPESQVSVKLKLQDGGIQQEWLMPLALVLNETLSNCFEHAF
                     PGRRSGTVEVTLSLSGDGGQLVVSDDGIGLAGGFATPSSQGLGLKILSVFAEQMRGRL
                     EIGASPQGGTKILLRFSMASTDI"
     gene            complement(19092..19532)
                     /locus_tag="PLP58_05935"
     CDS             complement(19092..19532)
                     /locus_tag="PLP58_05935"
                     /inference="COORDINATES: protein motif:HMM:NF014629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HRJ08088.1"
                     /translation="MQWRIITVGKPALPWAKAGVEDYLSRLRRMARVEHVAVKDGPRD
                     LVDARMLTASDDGLRIILDERGRALRSLELAAWTRRQEVCGTKRACLLIGGAEGHGEL
                     LRASADECWTLSKFTLQHEVALVVLAEQIYRAYSILRGEPYHRE"
     gene            19977..20483
                     /locus_tag="PLP58_05940"
     CDS             19977..20483
                     /locus_tag="PLP58_05940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ08089.1"
                     /translation="MTELPGYVRTPHTSPGRLVDVRGMAPGSKVVCPYTQKPFIVPGG
                     MRASSQPQVAARTTPRQQQPDTPVTASPRQTPASTPRITPPVTTGNVAALTRPAPEPR
                     LDPAPAAVPAPEPAAGLPYGSSIPGRPGFVNSPYAAKHQLVDVTGLPVGMEVKCPYTG
                     KLFRVPPQ"
     gene            20589..24329
                     /locus_tag="PLP58_05945"
     CDS             20589..24329
                     /locus_tag="PLP58_05945"
                     /inference="COORDINATES: protein motif:HMM:TIGR02604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HRJ08090.1"
                     /translation="MRFLILSLLCLMTTGLSGQGKELTGTYLPSTNACPSPEEARKKM
                     TVPEGYEVRCFAHEPMVQNPVAMTWDHRGRLWVVEAYEYPEGSKHPAPFGGEAKDDRY
                     HPMPGRGGEIPRDRVIILEDTDNNGEADKRTVFVEGLNLASAILCGDNGIYIGQQPHL
                     LHFRDDNGDDKPDAWRVVLTGFGREDTHELLNSFCWGPDGWLYMTHGVFTNSKVRRPG
                     QPESEGFKFDAGIGRARPVGQASSLSPSAAGILPAQDQPGKMPGTPEAGKMPALPWEF
                     EVFADGTSNPWGCDYDAAGNFFVSACVIDHFFHMAPGGIYVRQGGAPENPYAYELLPS
                     IVKHKHFRAAYAGVQIYQGGRYPADTHGHAFIGNIHDNAIHEEVLTPVGATFKCEPRR
                     DFLRANDGWFRPVSTQTGPDGFLWIMDWCDKYPCYQNAKANPEGVDRQYGRIWRVVYK
                     SEVGVQASADKNALAPPSHPTSPKGSTPTRPTNDLDLKKLSTADLVKTLEHPNNWMRR
                     MARRMLVESVGEQDRSLLSLLKDNMPNSADYAAIEVFWTALASGIIDFDLIKAALSSN
                     RNDLNHWAARAFGEHEPNAPKLLVGLAEHQEPFVRLAAAVALRQYISGSLTVNHRRKV
                     PVQDHADILIATRSALIRSSSDADPVLAHAIWMLIEPAADSFRSSALGESRNAFLEEM
                     ASGAAQSQPLSRVLSQKAMRRLCDTRKAENLDLAVEFCDKIAEHDILLAHALDGLVRG
                     QEGGVIKPVKEVSASLAKWRSSSNADVRKHAQTLAVMWGDAAAVKEVIAVLHDGAKSE
                     KERITVLQSLQKVRTDAVKDGLKPLLTPQTSTALAVAAIRAAADLGGDEFIAPLLQQS
                     ASKDFNVRNAAIEALSNRDTWTKALLSAISEQKVSASGFPAPVRRALATHKDKGIRDL
                     AFKVLGAWQDSSDDVKSLIAQKKQACLTGEPDLANGKLLFTATCMVCHEFHGGGQKVG
                     PDLIGSGRSNLDALLANVIDPNQIIGNGYENISVSTKDGRTLAGRIVEDTPGHVKLLG
                     AGGAAQVVPRDQIASLTNTKQSLMPMGFGNLPDAAFRDLIWYILAPPEEGPLTPEKKK
                     LLIQGVEVPETASQPKKTNWRAIDWESVSLWNPDWKVSAPDFERTPVKLAEYHGKTNV
                     LLVHPFPDKKTPASFERQLKLEKTKLKFSVAADDRGDWLAKVLVNGQEVKAVTVDHEK
                     PRWKTVEVDLTKWKGRDVTLRLEAHANGWAWEFAYWHGITLE"
     gene            24465..24695
                     /locus_tag="PLP58_05950"
     CDS             24465..24695
                     /locus_tag="PLP58_05950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009959875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046914 - transition metal ion binding
                     [Evidence IEA];
                     GO_process: GO:0006811 - monoatomic ion transport
                     [Evidence IEA];
                     GO_process: GO:0055072 - iron ion homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FeoA family protein"
                     /protein_id="HRJ08091.1"
                     /translation="MPLTLAQLPVGQQAVIHSMPTGRAGLTRLRELGLTPGARVRMVR
                     RAPLGEPIEITVRGSHIAMRNHEAADIAITLE"
     gene            24692..26863
                     /gene="feoB"
                     /locus_tag="PLP58_05955"
     CDS             24692..26863
                     /gene="feoB"
                     /locus_tag="PLP58_05955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009959876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015093 - ferrous iron transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein B"
                     /protein_id="HRJ08092.1"
                     /translation="MSDAAPPLLAVVGNPNSGKTTLFNLLTGLRQKVANYPGVTVEKK
                     IGECRTQHGKKLRVIDLPGAYSLNARSPDEAVLRDVLLGRRADTPRPDRILFVADASN
                     LERNLYLLSQVLELGLPTVLVLNMMDVAAHKQWRIDIPRLSEELGVPVVPMQAISGQG
                     FLELRVAISREDLPAPRWHSAPLPEDIRAALHRSLDSLASAGAIHDKATLLEPLYLMA
                     DHNPLHDGFEDSHLAQIREAREVLEKAHPGWEDQLVADRYAAIEKLSAKVLTLPEQEV
                     RTFTRRLDQFLLHPFFGGAVLLGILAFLFFLIFRVAEGPMGWIEESFAALGNTVTGLM
                     PPGDLRDLIVDGAINGVAGVVVFLPQILILFFFIGIMEDTGYMARLAFIMDRLMSVVG
                     LNGKAFLPFLSSYACAVPGIMATRTIDSPKDRLITILIAPLASCSARLPVYLLLISAL
                     MPESVSAWARAGTMVGLYALGTLGAFGFAWIFNKGILRGANAPMILEMPNYKLPSLKA
                     ILLQVWQRAKAFLVRAGTVIFGMSILIWAASTYPKDSALLAEAEKIEQRIESLETSGD
                     EAALTAAADELAIVKSRQLAQSLAGRAGHLIEPLIEPLGYDWKIGIGLIGSFAARELF
                     VNTMAVVYAVGAEDEEDMTPLRERIQQETRADGTPVYSPLVCVSLLVFYVFAMQCVST
                     LAIVRRETGSWKWVWFQLFYLSGTAWVLAFAVYQIGGMMGW"
     gene            26977..27459
                     /locus_tag="PLP58_05960"
     CDS             26977..27459
                     /locus_tag="PLP58_05960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ08093.1"
                     /translation="MESHQVIRQAVESSNVKKVASQLGMSLSLVYKWMEKDARNQSGV
                     LNPLDRIHQLYEITGHDHLIQWLCHKAGGYFVKNPPTGKRPGREVMPATQEIVQKFAD
                     VLSAISDAAADNSISDEEAKLIRHEWDELKRLTERFVKWCEEGDFEHLSEDLASKHLR
                     "
     gene            27470..28354
                     /locus_tag="PLP58_05965"
     CDS             27470..28354
                     /locus_tag="PLP58_05965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009965623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HRJ08094.1"
                     /translation="MPDLKTYLAERCAFVDSMLDRLIPSADTAPSTIHRAMRHSVFAG
                     GKRLRPILCLAAAEACGGGKESAAFAACAVECLHTYSLIHDDLPSMDNDDFRRGVPTC
                     HKVFGDGIAILAGDALQALAFEILVKTPVTVRHSAGALVAELARTAGSLHLVGGQVAD
                     LEGEGQKLPLEALRFIHENKTAALLATSLKLGGMSADCQPEELAALHDFGMAAGLAFQ
                     IIDDILDVTQTSEKLGKSAGKDLASEKSTYPALIGLDASRDEAHRLTRVAHDSLAIFG
                     PRGARLRELADHLLAREY"
     gene            28372..29427
                     /locus_tag="PLP58_05970"
     CDS             28372..29427
                     /locus_tag="PLP58_05970"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRJ08095.1"
                     /translation="MDDFLVIESVSRSFGRLRALDDVSLAIREGEAFSLLGPSGCGKT
                     TLLRLIAGFDAPDAGRILLGGRDITALPPERRPVNTVFQNYALFPHLSVRENIAFGPR
                     MAGWPRERVRESVEQMLALVKLESHAGHRPAQLSGGQKQRAALARALVNRPRVLLLDE
                     PLAALDLKLRQHLLVELGALHREVGATFIYVTHDQSEAMSLSHRIAVMNQGRIEQIAA
                     PEQLYARPATDFVASFIGDTNLVAGRITEDGNAVRFADGTLLPLPEKSAHPPGTLTRW
                     SIRPEHVRCGPRPPSDEPAVVSEVIYLGTHTHVWIESGPLRWLARMQGPPAFQRGAPV
                     SLTLDPAGACELPAPAS"
     gene            complement(29798..30244)
                     /locus_tag="PLP58_05975"
     CDS             complement(29798..30244)
                     /locus_tag="PLP58_05975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ08096.1"
                     /translation="MKRLTLRLAFPLLTATAHAQSYSITSRIIAGGGGTSSGGAYAVT
                     GAIAQPEASPPSTGGGYGISGGFFGQYMALQQTGAPHLTIRSVGGNVQVVWGSNVPGW
                     VLQANNTDLAPENWLDVAGTPTVMGAEQFLQFAAGGGRVFFRLRKL"
BASE COUNT         5476 a         9729 c         9675 g         5536 t
ORIGIN      
        1 ccacctccga ctacgaccgc gagaagctcc aggagcgcct cgccaagctg gccggcggcg
       61 tggccgtcat caacgtcggc gccgccaccg agaccgagat gaaggaaaag aaagcccgtg
      121 tcgaagacgc cctgcacgcc acccgtgcgg ccgtcgagga aggcatcgtc cctggcggtg
      181 gtgtcgcgct gatccgcgct caggccgcca tcggcgacct cggcctcgtc ggtgacgaga
      241 agaccggtgc tgaaatcgtt gctcgcgcga ttgaagcccc gctccgccaa ctcgccgcca
      301 acgccggcgt cgaaggcgcg ctcatcgttg ccgaggtcaa gaaaggcaaa ggcaacagcg
      361 gctacaacgt cgccaccgcc aagtatgagg acctcattaa ggccggtgtc gttgaccccg
      421 ccaaggtgac ccgcagcgcc cttcagaacg cggcctccat ctccggcctg cttctgacta
      481 cggagtgcct catcgccgac atcccgaaag aggagaaggc ggacgccggt cacagccatg
      541 accacggcgg catgatgtaa aaagcattga ataaaaagtt cgttttgaac tctcaccacg
      601 gcggcttgtc ccaaacaggc gggccgccat ccgcaaaacc tttgtcaacc agggctgact
      661 gagcaaaccg ggagctgctc tttcagccag gactggcaaa accccaaacc cccataaaca
      721 ggccggaagg aaacttccgg cctgttttct ttgtgcaaaa gaagggcgga agtggctttc
      781 aggcgcgcga acggttcagt aaagggagcg cacaccctca tcgaggttga agagagcctt
      841 gacctcgtca tcgcccaggg cgcggttaaa gacggccagg tcgtcaagat gccccacata
      901 ggccgcgccg cagaccagcg ccacctgggc gggatcccag ccaagcgtca ggtcccagcc
      961 ctcaatggct cccttgcgct cgccgtccat gtgcagcacg ccggccggtt tggcgccttt
     1021 ctcattggcc ttgtcgaggg tgaagaccac atgagtccag cgctcgcggg aaaagggcgc
     1081 gttggcgagc tgcaccatgg gccgcttctc cgccgggatc tggtcccact gcaggttgtc
     1141 tggattccag atttcgtgga gaggccgcac ggcgtagcga aaaaagcgcg gcgtctcgtc
     1201 cttggaccac tcgaggaaaa tgaagccctt tttcgtgtca tccccgatga tttgcacggg
     1261 gtcgcagtag cccggctcca ggtcctcgtc gggtgtgagg cgcagccagg cggagacgct
     1321 gacgctccag ctttggttgt tgtagccgag gatgccagcg ccggcgaact gcggccgcgt
     1381 ggcgcctttt ttcagaaaat gcagcgcgcc gccgaacctc cccgcgtttg gcgcgatgcg
     1441 cacctcgtcg ccggcgctca ccggcacgag ttccttgccg cgtttcgtca cgcaggtttt
     1501 ggatcccttg gaaaaatccg cgtcgaagcc cgtgtcgaag ctggcgtgaa aggtgagagc
     1561 ctgcttgagc gcttcggcgg cttgcgcacg cgcggcgcag agcgggagca gggtggacag
     1621 gagcaggagg cggagcattt tcatgggatg cggaatttcc tggcaaggct atgaaaccgc
     1681 gtccgcaaaa gccagcctgt tttttttatg acactcgaca ccctccgcac gctctgcctg
     1741 tccatgccgc aggcggagga gacgcagccc ttcggtccgg atgtgctggt ctataaagtg
     1801 gcggggaaaa tgttcgcgct cacggtgcct gaggattttc cgccgcgggt gaacctcaaa
     1861 tgcgaccccg aacgcgccct cgatctgcgc gacgagcacg agtccatcct gcccggctac
     1921 cacatgaaca agcgccactg gaacaccgtg gtgctggacg gctccgtgcc ggcggcgctc
     1981 gtgcgcgaga tggtgcgcca ttcctatgac ctcgtcgtgg ccggtctatc cgcgaaggca
     2041 agacgcgcgc ttcagaccgg gtgagccgca tttttcctgg caaggcaggc gcaggacgcc
     2101 gtatatgcgg gcatgcatct cctcctgcgc gccctgctcg ttttcgccct ggctttcctg
     2161 gcttgctgct cgcgccggga cgcgctggtg gtgggcatgg acgcgactta cccgcccttt
     2221 gagtatgtgg acaaaaacgg gagcatcacc ggcgtcagca ccgccatcgg cgaggaggcg
     2281 gcccgcgtgc tgggcaggcc ggtggtgttt caaaacatgc ctttcgacgg cctcatcccc
     2341 gcgctgcaga ccgggcgcat agacctgatc atctcctcgc tcacggccaa cgaccagcgg
     2401 cggcagtcca ttgacttctc cgaaccttat gtgcgcaccg gcctctccat cctggtggca
     2461 aaggactccc ccgtgcaaag cgcggacgac ctgcgcgccc cgggtcggaa gctggcggtg
     2521 cgcatcgcca ccacgggcga gcagtggtgc cgccaggagc tgccgcaggc gcgcctcacc
     2581 gccctggaca cggacgcggc ctgcgtgctg gaggtggtca atggcaaggt ggacgcctgg
     2641 gtttacgacc aggtctcgat catgaactac cacatgcagc atccggagcg cacccgcgcc
     2701 ctgctgacac ccctgcgcga ggaatcctgg gcggtcggag tgaaaaaggg ccgcccggac
     2761 ttgatggccg ccgtcaacga ggctctggcg cgcatgaaaa cggacgggcg cttcaccgag
     2821 ctggcggagc agtacctcgc caaagagcgc gcgctcatga aggcgcaggg gctgcccttt
     2881 gtgttcgagt gacggcggtg cggtgcgtgg atttttgaat ttcggtttcc cgtttcccct
     2941 ctttaactgc gcgcatggaa tccctgcttc tgctcgcgcc attgccggag tttttgcttt
     3001 cgcctttgaa agcggtctat gcctgccatg acgggaccgc caggctggat gacgtggtgg
     3061 cggagcatgg ggcggaggtg cgcggcatcg tgatggcggg cggctccgcg gcgccggcgc
     3121 cgctgctgga gcggctgccg gctttggaaa tcctggccgt cttcggagtc ggttacgatg
     3181 gcgtgcccgt ggactactgc cgcgcgcgcg ggctgcgggt gacgaacacg ccggaggtgc
     3241 tcaccgacga cgtggcggac atcgccacag ccctggtgct gatgacggcg cggcggctgg
     3301 cggaggcgga gcggttcatt agggcagggg agtggagcgg cggggccttt ccgctggccc
     3361 gcgcgctgcg gggaaaagtg gcgggcatat tcggcctggg caggataggc gcggccatcg
     3421 cgcgcaggct ggaggcgcac gggatgcgca tcgcatatca cggcaggcgc aggcaggcgg
     3481 tggagcatga ctattttccg acgctgcgcg aacttgcggc ggcggcggat tttttggtcg
     3541 tgtcatgtcc gggcggggcg gagacacggc acgcggtgga tgccgaggtg ctggcagccc
     3601 tggggcctga gggcacgctc atcaacatcg cgcgcggcag cgtggtggat gaggcggcgc
     3661 tcgtgagcgc gctggaagcc gggacgatcc gcgccgcggg gctcgatgtc ttcgaaaacg
     3721 agccgcatgt gccggaggcg ctgctggcca gtccgaggac ggtgctgctg ccgcatgtgg
     3781 gcagcggcac gcatgagaca cgcatggcga tggcggcgct ggtggtggag aatctggcgg
     3841 cgcatttcgc cggcaggccg ctgccgactc cggtgtgctg aacgcaaggt tgcgccgcaa
     3901 acggcggcag tgcatgggtg tcgcgtgaaa ttcctgctcg cctccctgct cgccgcgctg
     3961 atgtccttcg catggggcct gctctcgcgc gaggtgatcg gctggcgggc cacgggcgag
     4021 cacgcttttc tcaatgagtc cgccgtggcc gaggcggtgg cgaagggggc gaagaacggc
     4081 aggggcatct acctgctgcc atatccatcg aaggcgcccg ccttcggcag gggcgagcag
     4141 gcgaagatgg acgaggcgca cgccaaggcc ctcaagcgcg gcccttacat gcgcgcggtg
     4201 gtgcgccctg gcgcgcgtga aaggaacttc ggcgtggatt tggcgtggag cttcgcgcgg
     4261 tcgctggcct gcgcgctggt gcttgcgggg ctgctctcgc cggctctgct gaattacgcc
     4321 gggcggctgg ccttctgcgg cggggcgggt gctttcgcgg ggctggcgtt catgctgccg
     4381 gagatgattc actacgaact tccggcacgc gcggtggtgg tggcggtggc cgacggcttt
     4441 gtggagtggc tgctggccgg ggtggtgctg gcatgtttcg tgggtcggga gccgacggcg
     4501 cgggatgtgg tgtgagccgt cattttaggc tcgccaaacc agatgagggg catcagaatg
     4561 ccgattctaa aaaatacccc ggaggtttcc catgcccaaa ttccaaaccc gccctcttgt
     4621 caaactcgcg ttttcgctcc tgccagccct gtttcttgga ctgctcttgt ggtggcttgc
     4681 acgtcaggaa aaaacaacag cgcctgatca acccggcgtg gtgcaaacaa gcgccaccgc
     4741 gccgtctccg cttgtcgcaa ccgcgcccgg catcggcgcg cggatggatg aaaaggatgt
     4801 cttcaccaag tttgaggaat gggccgcgaa tttcgcggga ggtgaagcct cggcaggggt
     4861 ggaactcgcg cgcgagcgcc gtgcggcgct gaagaccttg atcatcagcg acccgaaggc
     4921 cgccctggac cgcgcgctgc cgtggacgct gcgcaggcgc atgccgcatg aggtggcgga
     4981 tttgctggag gagcgagtgt cggcaagggg cgagttttcc gtgatggcgg gcctgcctct
     5041 gccgggcacg gacgcgcggc ccttttacca gcgctcgttc acagtcaacg gcgggcgcag
     5101 ctatgacgcc tacgtttacg gccgccgcga gcatgtgggc acgaagtatg acttccctgc
     5161 ggacggcatc gcggtggatg aggctctggc gctgcgggaa cgtcccgcgc gcagcccgga
     5221 gccggaggag ccggcgcagc ccggcgaggt ggtgcgggtg gcgcaggacg ggcatgaggt
     5281 ggcggccaat ggcggtgtgc cgctggtagc cggcggcacc agctaccggg tctgctgcat
     5341 ggcccacgct ggagcggtgg agcaaggctg ggtggaggcc gagcaaagca ccggaccgtg
     5401 gatggacccg gatgtggccc cctcggtctg gacggaaggt ccgaaaacgg cgctggtaat
     5461 ccgtgtggac ttccccaatc tgccgggcac gccgcgcaac acgaacaacc aggagctgac
     5521 cgtggattat gtcaccacgg agatgaacgg ggatgtggcc aactacatga gggacgtgtc
     5581 ctaccgcaag accagctaca ccaccgccac cacggatgtg accgccgtgc tgcggatgcc
     5641 gagcaactcg accttttact caggcagcgg cggcgccaac gccctgcgtg ttgacgcgct
     5701 gagcgcggcg acggcggcgg gcttcaatgt caactcctat gaccgccagt tggtggcctt
     5761 cagcagcatt ccattcccct gggccggcct tggccagatc aacggaccct tcacctggta
     5821 caatggctat ctccaggact tggtgatcat ccacgagctg ggtcacaacc tgggtttgcc
     5881 tcatgccagt ctgtggcgcg ggacgggcgg caaccccgtc tcgacgaccg gcacgctcca
     5941 gtcgtacggc gatccttttg actgcatggg caacaacttc aacggcccgg tgccgcgcat
     6001 gtggttcaac gcatggtacc tcaaccgcat ggactggatg cccaacgcgg cggtgaccac
     6061 agtgaccgtg ccaggcacct accgggtgta tcgttacgat gatccggacg cgccgctgaa
     6121 ccggcgcctg gcgctgcgtg tgcaaaagga tgccacgcgc agttactggc tggcctaccg
     6181 gaggcagttc gccggacttg ccagcggact tgcggacatc tcggcgggcg cgcaggtgag
     6241 ctggggcttc ggcaacactt acaatgacag ccatttgatt gactgcgaca ccccgacgac
     6301 caacgcgcag gatgcctcgc tcaatgtggg atccacactc atcgacttgg atgcggggat
     6361 atccttcaca gttcaggccg ccggcggcac gggcgtggat gcctacctgg acatcagtgt
     6421 cgtgcagacg ggccgcattt acccgctcca gactcagtat gacgtggacg aggcggcggg
     6481 aacggtgaca gttcaactgg cgcgcaccgg cactccgagc cagggcgcct cggtgacggt
     6541 gacgacccag aatggcacgg ctgtggcccc cggcgattat tccagtgtca gcaccacggt
     6601 cacctggagc ggcacggaca tcactccaaa accggttgtc atccccgtcg ttgcggatgc
     6661 cgtcaaggaa agcgcggaga gcttttttgt gaactttgaa cccgcgccgg gaagcgggcc
     6721 ggaggtcatc gtggtgggca gccccgtcac ggtcacgatc cgcgagcccg gcatgaggga
     6781 caacaccttc gcccacgagg agttcgagga gccgggcagc gtcaaaaact tgctggccgc
     6841 cccggacgga cgcgtcatct tcaccggcga ggcgaccgaa ctggatgatg aggatgtgga
     6901 cggcctgggc aggctggaaa atgacggcgc gctggatgcg accttcatcc agggaggcgg
     6961 catgctcccg gtcgcagggc gcgtggtggc gcgccagccg gacggcaaac tgctctttgg
     7021 gggcaacttt gtcgtgctgc gcgacgcgcc cgccaaccgc ctcgcgcggc tctttgccga
     7081 tggattctcg gacaacactt tcaacatcgg ctcgggaccg aacgaggagg tgcgcgcgct
     7141 ggccgtgcag gcggacgggc gcatactggt cggcggacgc ttcagcgtct ggaacggcgc
     7201 tccgaggctc gggctggcgc ggctgctccc caacggggcg ctggacaccg gattcatggc
     7261 ggcggcgctg ccgcacatct cggtcatgga ggtggaggcg ctggtgctgc agcctgacgg
     7321 caaggttctc gtcggcggtc tgatccacac ggagagcacg ggacagatct tcaccggcgg
     7381 cctgagcagc ggcgtgctgc ggctgcaccc cgacggcacg ctggacacca gctttgacat
     7441 cggcgccggg gcgcatctgg agggcaacac cggcacaccc gcgcgggtca gcacgctggc
     7501 acggcaaggc gatggcaagg tggtcgtcgg cggcgccttc acaggcttcc gcggagtgac
     7561 ggcacggcga atcgcgcgct tgaacacgga cggcaccctc gacattggct tccagtccgc
     7621 gatgggtgtc aacggcccca acaaccccgt gcgcagcgtg gccgtgcagg gcgacgggcg
     7681 catccttctg gcgggcgagt tttcccaggt ggccggagtc atccggcatc aactggcgcg
     7741 gctgctgccc accggcgctt ttgacaacgg cttcgacgcc gccgtgccgc tcggcgcggc
     7801 caattttggc aaccatgtga tccagcagcc ggacgggcgc attctcctgg ccaccgaggc
     7861 ggctggaacg gactccatct ggcgtctttt cagcgggcag ctcggccgcg cgggagtgat
     7921 ccagttctcc gccggcaacc atcaggccaa tgaaggcggc ggtgggcaga tcgaggtggc
     7981 gcgcgtgggc ggcagcctgg gggctgtctc ggtgaattac gcgacgcttc ccggaacagc
     8041 agggaccggt gacttcacac ccgtgtcggg aacactggac tgggtggatg gtgacacgac
     8101 atcaaagttc atcaccgttc aagtctccga ggacgccata ccagaaccgg tggagttttt
     8161 caccgtccag cttggcgtgc cctcaggcgg cgcctttctg ggggagacag ccctggcctc
     8221 ggtggcggtg ctcgacccgg gagcgtcgga ctttgcgcgt gtgcgcttca ccacggattc
     8281 cagccatgcc ttcgagttcg ccaccacgcc cgtactggtg actgtggaaa tgacgcccgc
     8341 caaggccgag gcggtggcag tgcccttcac tatcagcggc acggcgactc cgggcattat
     8401 cggtgactac gagatcacgc ccgcagtctc tccgctggtc ttcgcccctg gcgagacgac
     8461 gaagactttt tccatcacac ccaagcagga caaaatcacc gaaatcggcg acgagactgt
     8521 gatcctggat cttggcttcc cgcagggtga ggcgctgctg caggcgccgt tccgccacac
     8581 tttcaccctc gtggatgacg acacggcgcc ggatgtcagc agtgactcga acaaccagat
     8641 cgtgcaggcc gggcagttgg ccggaccctt tgaaggccag gtgtccggca gcgcgccgct
     8701 gtcactggag tggcagctca acggcaaaaa agtgcccggc gccggccagc cccatctttt
     8761 aatccccgcc gccagagtgc aggacggcgg cgagtaccgc ctggtggcca gcaacaagat
     8821 caccaaaaac atccgcggcc aggcctccca cctcgtggtg gtggacaccc gcgtgcgcac
     8881 cgtggtggtg ccgctgggcg gcaaagccgt gtttttggcg gaagccggcg gcaacgccct
     8941 cggcttcgag tggcggaagg tggacggacc gtttcctttc acccgggcga gcacccgctt
     9001 tgaccggaag ctgacccttt cgaaagtcga gctggacgac tcgggcgtgt acatctgccg
     9061 agtcagcaac tcgcgggccg ccacggcggg cggtgatttg ttcatggacg ccggtccagt
     9121 ggtgctcaag gtggtggaca ccgctccgct gatcggcctg gtggaaaacg agctgctgcc
     9181 gccggccacc gtgggcggca cttacaccta tgaagtgccg gtggatcccg aagtgcgcaa
     9241 agcgcccatc aagtggaccg ccgcaggtct gccgtccggc ttgaagctgg atgcggtcac
     9301 cggcatcatc agcggcaggc cgcaagcgcc ggacaagaac ggcaagccgt atgacgtgat
     9361 actgacagcg gccaacaacg gagggaaagg tgtccgcaag gtgcggctca gtgtggccca
     9421 gatgccgccc ggtctcgccg gctcttacac ggcacgtctg gagcgtcaca cggccatcaa
     9481 tggcggcctc ggcggcaggc tggacttcac agtcacgccc acgggttcct tgtccggagt
     9541 gcttttcctt ggtggcacgg cgctgaaatt caccggcgtc ctggaggtgg atgtgaatgg
     9601 cgtggcgctg ccaaaagtgg aggagctgcg catcgcgcgt ccaggcaagc cggtggcgcc
     9661 gccgccgctg gtggtggggt tttccatcga ggacaacctc gtgaaggcgg gcacgatcag
     9721 cgatggcgcg cagacgctgg acttcaccgg ctggcgcaac gtctggagct tcgccgccaa
     9781 ccgcgcggac aagttcgccg gcccgcacaa ttttgtcctt gagatgccgt ccagcattcc
     9841 tgacgtgccg cgcggctaca gctatggcac ggcgcaggtg tcggcctccg gcgtcgcgac
     9901 ctacaccggc atgacggcgg acagggaaaa aatcacaggc tcggccttgc ttggcccgga
     9961 cggaaagctg gtcttccacc aagctctata taaaaacaca ccgcaaggat cgctcaacgg
    10021 ggagatgctc attgaggagg gagcgctagc cgatgatccg gcagacaatc tgctcagcgg
    10081 atcgctaagc tgggtgcggc ccgcgatgtc caaaccgcgc ctttacgcgg caggattcgg
    10141 tccggtggat gtggctgttc tgggcggaag gtatcaagcg ccagcgctgc cgctgggcct
    10201 ggccggtccg ggcagggtgc ggctggtctt tgatcacgcc ggactgccgg cctcgcgcga
    10261 gccgggcatc accctggatg tggatgcgaa aggcaagctc atcccgccgc tcaaggagga
    10321 caatcgcgcg ggcacgacct tgaagatcac ggcggccagc ggcttgttca gcggcaagtt
    10381 ttccctcgaa gaccccaatc cagtgccgcc gccagccttc atgaagcgcc caacaacctt
    10441 tcagggcatc attgttccgg tgggaggcga gctgcgcggt tacggctggt tcatccttca
    10501 gcaactgcct gatgacatgg aaggcacgac ggtgaaaact tccccgttct tgtcgggcga
    10561 ggtcatcttc gagcaagtgc cggtggtgac gactccctga gcatcatgcc gtttcctccc
    10621 gcgcttcttc ggaaggcgct ggagcgggag gtgtgtgcgc ggccagcggc tcaaggatgt
    10681 gcgtggccca ggggcggaag cgctccagaa tcccgggtcc gatctcggtg ctggtctcat
    10741 agccgcggcg cagcatctcc agcttggcgt ccagattgtc cacataatgc agcgccacag
    10801 cctctggggt ctttggcaga acgggcgacc cgtattgcag ctctccatga tgcgcggcga
    10861 tgagatggag caaatgcagg cgtgccagat cgctggcggg ctccagcgcg gtccactccg
    10921 cggcttcggg gaggtccatc agctcgcgcc acagcttgtt caccagctcc atgcccagag
    10981 ggatgtggcc gatcatctcg ccgtggagct ggtagggcat ggcgaagccg ctctccgggt
    11041 agcagttttc ccacagcttg ccgcagtcgt gaaaaagcac gccggcgagc agcaggtcgc
    11101 ggttcagcat gggataaacc cccgcgattg cagcggccgc gcgcatcatc tgcgccacat
    11161 gttccaccag gccgccacgg cgcgcgtggt ggttctcccg cgcggcggcg gtgcggtgga
    11221 agcgcgcgcc gtgtttttcc aggaacaggc gggccaggcc gcgcaggcgc ggatcggcca
    11281 gcgagtccat gaatgcctcg atttccgcgt agtcctgccg ctgcctctcc gccagcgctc
    11341 tgtcgccttt gagcaaatcc cgggcttcat catcggtcag ggggcgcaac ctgggatggc
    11401 gcggctcggg accatatttg ccgccgtcca cccagttccc cgaaagctgg atgaactgcc
    11461 cgcgccgcag ttcgcgcgcc tcggccaggg caggattgtt gtcgaacaca cgccagacga
    11521 gcgagcctcc tgcgtcggcc agcttgatct caagataggg gctgccggtg ctggtctttt
    11581 tttcctgatg caggtccact tgcgcatgca cccggcatgg cagcggagcg ggtccggcgt
    11641 tttggcgcag ttgcgcaagc gtcagcagtt cgaaatcatg ctcatccatg cgggcagtca
    11701 agcggtcggg gcggaggctg gcaagagaga aaggcgcgcg cgttgacatg cgctcgcccg
    11761 gatgatctac tgggcttcac aacctggaat gcgcgaaagt ttcgtgacat tcaccctccg
    11821 agatcatgga cactatccct tcctaccgcc agcggctccc gcactggctg aacctctgga
    11881 tgcccttgtt catcctggtc tatcccttca tcctgtcggt gccttctctg aattgggaga
    11941 aggacttgta tcgcgagtac ggtttgattg aaaatctgac ggtggctttt ctcgccgcgg
    12001 ccatcgtgct gtttttgcgc tccctgcgcc gcgccgacgg cctcgttcac cgtgcctggc
    12061 tggtgctgct ggcggctgga gcgttcgttt ttcttggcga ggagatcagc tggggccagc
    12121 attactttaa ctgggtgact cccgaggaat ggaaggagat gaaccgccag ggcgagacca
    12181 atctgcacaa catcaagggc ggagtggagt atttcttcac caaggtggcg cgcgagggct
    12241 tgtctctggc gaccatcatc ggcggcattc tagtgccgct gcatttgcgc cggaaaaaag
    12301 cggatttcac accgggaacg ctctggttct gggtttggcc gtcgcttgga ggcgctttga
    12361 ccgccctgct gtcagccacc atccgcctgc cgaacagcat cgcgcatcag tttggcaaag
    12421 tggactgtct gcctcccttt cttggcgagg acattggcga gatgaaggaa tgctacctgg
    12481 ccctttttat cctgctttat gctctgacac agttccgcat cacacgcagg ctgccggcgg
    12541 ccgggacatc ctgatctgcc catgatgaca ctgaccacgg cggttctgtt gttcctggcc
    12601 ggcctttcgg cggggttcat tgacgccatc gccggcggcg gtggcctgat ttccgtgccc
    12661 gcgcttttgt gggcgggact gcctccgcag atggctttgg gaaccaacaa gatgcaaagc
    12721 acctggggca cgctgcttgc ggtggtgcgc tactcccgcg cgggcttggt ggattggagg
    12781 caaatgcggg ccggcattgc tgtcaccttc actttcgcac ttctgggcac ctgggtggtg
    12841 acgcgggtga gcaatgaggc gctcaaacag attgtgccct ggctgctgct gggcatcgcc
    12901 atttacactc tgctcagtcc gaggctgggc agcaggccgg cgcgcgcgcg gatggagccg
    12961 ctgccgttcg cgtgtctgat cggcgcgctg atcggttttt atgacggctt tttcggtccg
    13021 ggcaccggaa ccttctggac ggtggcatgc ctgtcactgc tcggtctgga actgacgcgc
    13081 gccacggcct acaccaaggt ggtgaacctg gccagcaaca tcgcctccct gctggttttc
    13141 gtcatcgcca tgcgcatccg ctatgatgtg gcggcggtga tgatcgccgg gcagctcatc
    13201 ggcgcgcgtc tcggttccgg gatggtcatc cggcacggcg cgccattcat ccgcgtggtt
    13261 ttcatcctcg tcgtgctggc catggtggcg aagctgcttc tggaccaatg gacggctggc
    13321 tgacgaaaaa aaggcaggcc ctgcggcctg cctccgaaca gcatcaactc ttggaaattt
    13381 tagacgaggc cggcggcctt gagtgtcttc agagcgccgt tcttgttcag cgttttcaag
    13441 gcgcgcgcgg tgagttttac ggtgacatat ttcccgagtt cgggcaccca gatgcgcttt
    13501 tggcgcagat tcgggtaaat gacgcgcttc acccgcttgg tgatgtgctt gccaatgccg
    13561 ccctccttct tcgctttacc gctgcgatga atgtggtggc cgcgcgtgac gccgactccg
    13621 gtgatttgac agactttggc cataatgaaa ggttcgttga aatttgaggg ccgccagagt
    13681 aacgcctgat gcccgtgcgt caaccgtcgg aaccgtcttt ttcacgcccg acttcatttg
    13741 cccacggcgc gctgggcgag gacggttttg accaggcgga gcatgtccgc aggctcgtaa
    13801 ggttttggca gcacggcggc gaagccatag gctgccggac gggccatgac ggggtcgtca
    13861 ctgtagccgc ttgagacgat ggcaagcacg ccagggtcga gctggcggag tttttccatc
    13921 gcgcgcacac cgcccatgcc gttggggatg gagaggtcga ggatgacgag atcgtaaggg
    13981 cgtccagcct gcgccgcctc ctggtaaagc ctgacagttt ctccgccctc gacgctttcg
    14041 gccacctcgt agccctggcc gcccaggttg cgcacgatga ggctgcgcac gagcgggtcg
    14101 tcctcaagca cgaggatgcg ctgcgtggcg gtctgcgggg ccgagtcgaa aagctggccg
    14161 ccaatgccga gcgagcccgt ctcgtcaccc tcgtcgtcga ggggaagata aagccgcact
    14221 ttggcaccct tgccaggctc gctgctgacg gagagggagc cgccatgcgc tttggcgatg
    14281 gattcgcaga cggtgaggcc gagtccggtg gcattctcgg atttgcgtgt gctgaagtaa
    14341 ggctcaaaaa catgcggcag cacctccgag gggatgcccg cgccctggtc atggacctcg
    14401 acgaccaccc cgcccggtgc ctgcacgggc gcttcgctca gaatttcgtg cgggaacatt
    14461 tcacgtgggt ccatgggatg gcagcgcacg gtgatgactc cgccctccgg ctgggcctgc
    14521 tcggcgttgc ggaccaggtt gccaatcagg cggcgaatct gcgcggggtc cacagccatc
    14581 gtgggaagcc gggggtggat gtcgagacgg tactcgatgc cggtggcacg cgtgtgatgc
    14641 atgaaaaact gctcgatgag atcggccatc tgcacgggcc ttttgatggg cgcgccgccg
    14701 cgtgcgaagg tgagaagctg ctgcaccaga ccctgcgcct ggagcgtggc ctgtttggcc
    14761 gtgtgcagtt cgggcagttg gatgacattg cggaggcgca tttcggccag ggaaagattt
    14821 cccagcagca cggtgaggag gttgttgaac tcgtgcgcga aaccgcgcgc cagcaggcca
    14881 agggactcca accggtccag gcggtggcgg cggtcggcct cctcgcgccg ggtggtgatg
    14941 tcctgcatga gcacgagcag gccctcggca aaggggtgcc cgcgcagctc cagccaggtt
    15001 tgtttttcct cgaaatagac ttcccggctc acgctttccc ggtgcagcag ggcgtgggcc
    15061 agcgcctcgt ggtgcgcctc gcgggtggcc ataggcagca agtcccagaa attgacgccc
    15121 atgagcttgt ctggtccgcg ctcgaacagc cgttgcgcgc tggcgttggc atagatcagc
    15181 cgccagcgcc cgtccagggc gatgagcggg tccgagatgc tctcgaccac gtccaccagc
    15241 ggcgcctggg cggtggcatc aggcgcggca gcgtgtgcgg cgtcggattt ttccgcctca
    15301 gtgacgggta gggagcggaa gagaatgacc actccggtga gctgcccggc ctcgtcgcgc
    15361 aagggggcgg agcggtcctg gatgggcacg cggctgccgt cgtgagtggt cagccagaca
    15421 gtgcgctcgg cattggcgcc gccgccggtc agttcgacca tggcgggttc tccgctggcc
    15481 tggtaaatgc ggaacacctc gtgcagtgag cggctcacag cctgggagcg ttcccagcct
    15541 gtgacccgcg cggcggccgc gttcatgaag gtgatcacgc cggcgagatc ggcggcgatg
    15601 actccgtccg cgaggctttg gaaggcttcg aaacaacgct gctcgcgcag atgacgctcg
    15661 cgctcttttt gcagccgcgt caccgccaca tccactgtca ggcgcatatc gtcatgattg
    15721 aagggtttga gcaaatagcc gtaggggtcg gtgtcgcgcg cccggttcac agtgtccagg
    15781 tccgcgcagg cggtggcata gacgaccgcc accggaccct gctcacgaag acgcctcgct
    15841 gtttcgatgc cgtccatcgt gccgtcgaga tgcacatcca tcagcaccaa gtccgggcgc
    15901 agctcacggt tcagtcgcag cgcctcctcg ccagaggcgc aagtgccaac gacccggcat
    15961 ccccagtcgt tgaggcagcc agccatgtcg cagccgacaa gaccctcgtt ttcgacaatg
    16021 aggatgcgta tgcggtcaaa gccgttcatg attcatggaa gtatgaatca tgctagctct
    16081 aaatatcagt agatgccata gaaaatcgca acagaatttt cgttcccccc tggggtgagg
    16141 ctccgatttc cagccgcccg cgcatttgct cggcaaaaac agagagtatt ttcaagccta
    16201 gtccctgact ggagggtgtg gcgaatccgc ccgcaagtcc gatgccatca tcactcacaa
    16261 cgagctgccc gccatcgccg ctcaacgaga gcgtgacttc caccgtgccc gagcgacggc
    16321 ctggaaaggc gtgctcgaag cagttggaaa gtgtttcatt caagacgagg gccaggggca
    16381 tcagccattc ctgctggatg cctccgtcct gcagcttcag tttcacgctg acttggctct
    16441 ccggcacgtc aaaacaatca cgcagcctct gcacgagacc ggcggcgaag tctgcgaaac
    16501 ttgagttcga tccaagagcg agctggtaga gatgctggtg cagttcggcg atggcgcgca
    16561 cacgattctg gctggacctc agtgcgccgc gcgccgctga gtcctggaca gtgttcgact
    16621 ccaggttcag caggctggag atgatttgga ggtggttttt gatgcggtga tgagtctcgc
    16681 tcagcagcag tttctcccgg gaaagatccg ctgccggcca gggtttgagc gctggcgagt
    16741 ccgcagttga tctgttgggt ggcgctttgt cagaaagctg ccgcaagtcg tctcctccgg
    16801 cttcaaacaa ctccacatcg aaaccgccgc cgccgcacgc gcgagccagc aggcggcacg
    16861 aggcgcgttc gccgccggct tggaggcatt cgaaagtgcg ctcatcttga gaatttgccg
    16921 ccagttcgct gtaaaaaccc gtcgcgtctg atgggcggaa atgcagatgc agggcgcggc
    16981 cttttgcctg gccggcttca agccctagaa gctccagtcc gcgaggactg caatcttgga
    17041 tcctgccatt gacgttcgtt ctcagaagaa acactcccgg accgccagag gccgggggga
    17101 tggctgacgg cgcttttgtc ttcaaagagg tcgtgctcgt gccgccctcc agtttttgac
    17161 cggtctgcgc gcttccggca ggttgcagaa aatgtaccgt gttgtgcggc aggccgtcag
    17221 catccagaac ctgtgcgagc gtcattgcgc agggcatcgg atcagcgccc gcccggacta
    17281 agcggatatc aaggctggcg gagcggtccc ctgaggcttg caactggcgg agcgtctctt
    17341 tcctgcgctc gatgtcgggc gcgtccatcc agccgccggg gggcagccgc cgcagttccg
    17401 acttggagcg gccgagcatg gcctcggcgc gcgggttcgc ctcaaacacg gagccggttt
    17461 tgtcagtcag tattacggcg acaggcgcgt cgtgaaacat ggcgcggaac ttggcttccg
    17521 aggcgcgcag ttgctcctcg cggcggcagt gttctgtggc gtcctggatg cagacaagca
    17581 gccctccgcc gggcagcgcg cagggttgaa actccagctc tttgagcagt ccgtccgctg
    17641 tcaccaggga gaatatccgg gtcaactgcc tccgccacac atcctcgcgc cagatggtgg
    17701 aagtctcctg ctttccttcg tcccctggac aggcggcggc cagccagttt tcgaccgtct
    17761 cgtccttcct cacaggcctg cctagcaggc gggagagggg ttcgttctgg aaaaccggcg
    17821 cgcctgactc atccagcacg agcacaccgc agggaagcag gtcgaggtat tgccgccccc
    17881 gcgtttcttt ttgtgcgaaa agatgctccg cctcgcgcag cgtgcgcatc tcctggcggg
    17941 tgcgcgccag atcatcctgc aggcgccgca cctccaggtt cgcggaggga tcggcaggcg
    18001 tctgctggga aggctggcgg atggaggcca cggcactggc gatggcgggc agatccgggg
    18061 cggcaggcat cgcggcagga cggggcacag ggtcatgtcc ttgtccgcgc ggcatgccca
    18121 cggcgaggaa gatcatgcgg tcgcctgatt tgccggaggg actcagtagc caccgctccg
    18181 gaagttccga ctctccgctc atgcgctcgt gcgcgccggg aaccggcagc atggccccgg
    18241 aagagccgct ctccagtttg tcgagagcct tttgccagag ggactgaaaa gccgcgatgt
    18301 ccctgccgct ggtgaacatt tccgcaaaca gtgacgcgcc ctccctcaaa ccgcgcgcca
    18361 ggcacagttc gacagccgca gtgttggctt gatgaattac acaattttca tccaccagaa
    18421 ccagaggcag tgcgcttgga ggcatttcaa tatccggagc gtgccgcacc tgctcgctgc
    18481 gtgagtccat gggcagttcc ggggggaggg cggaaacacc cgatggcatg gcggcgaatt
    18541 tttcacaagt caccatcagc ccccccacac tggcatcctt gtttcgtctc caggggcgca
    18601 cttcccagcg gtaaaccagg cggccgccgt cctcgcctcc tatctcatca tgctcgctgc
    18661 gcacgacatg gccgctcagg ccgcgctcat acacttcccg ccagcctgga tgcatggcgg
    18721 ggaatatttc atattggctg cggccaatca agggttgcac ctcctggaga gagaactcct
    18781 caatccatgc gcggttcgcc agcacataac gcatctcgcg gtcaaacatc gccatcgcca
    18841 caggcgcgtt ttccacaaga actccaagca cggtttccat ctccaggtcc attccgtctt
    18901 gtggaaaatc ctcatccggc tcatgaattc cggattttcg tccagcctcg ccacctattc
    18961 catgaggagg atgaatcaca ttctggagga cggaaccgga catgaggtga gatcaggagg
    19021 aaggattgat attttttaaa tattagcaga cctcaagttc tcaacaaggc gaaacctcga
    19081 agaattgcga ctcactcacg atggtaaggt tcaccgcgca agatactata ggcgcggtag
    19141 atttgctcgg ccagcacgac aagcgccact tcatgctgaa gcgtgaactt cgacaaggtc
    19201 cagcactcat ccgcgctagc gcgcagaagc tcgccgtgac cctccgcacc gccgatgagc
    19261 aggcaggccc gcttggtgcc gcaaacctcc tggcggcgtg tccatgccgc caattccagg
    19321 cttctcagcg cgcgtccgcg ctcatccaga attattctca ggccgtcatc agaggcggtt
    19381 aacatgcgcg catccaccag atcgcgcggt ccgtctttga ccgccacatg ttccacccgc
    19441 gccatgcgcc ggagcctgga gagataatcc tcaactcccg cttttgccca gggaagagcg
    19501 ggtttgccga cggtgatgat gcgccattgc atggccttaa gtagacacga atgccagttt
    19561 ttcaaccaga cgaagaagta tggaaaatca aaataattgt atctcttgat ggtcctggga
    19621 catctttgag gttgcagaat ctaaaaaatt cactattctg agagaggtaa ttcaaaaaat
    19681 tgctctccgc catgaaatac gcgcttactc cctctcgact tggactcccg ctcgcgtcca
    19741 ttggactcct ggctctggcc tcctgctcca tgccgcctcg tgacgcatgg aaccaaatcc
    19801 aaagccgcgg cttgatcagc tattacacca gcaaaccctc tggtgcaccg ctgaccacga
    19861 gcagcagcaa tcgcatgttg actgcaacac agcagactcc cgcctcttat gtccgcagca
    19921 atccctccgc cgcacccaca cgcagtagcg agggtgctct tccgttggcg cgccctgtga
    19981 cagaacttcc gggctatgtg cgcacccccc atacttctcc aggacggctc gtggacgtgc
    20041 gtggcatggc tcctggatca aaggttgtct gcccctacac ccagaagccg ttcatcgttc
    20101 ccggcggcat gcgcgcttcc tcccagcctc aggtggcagc gcgcacaact ccacgccagc
    20161 agcagccgga caccccggtg acggcgtcgc cccggcagac gcctgcgtcc acaccgcgca
    20221 tcactccccc tgtgacaacc ggcaatgtgg cagctcttac ccggccggcc cccgaaccga
    20281 ggctggaccc cgcccccgcc gccgtgcctg ctcctgaacc tgccgccgga ctcccttatg
    20341 gatcgtccat tccgggccgc cctggtttcg tcaacagtcc ttatgcggcc aagcaccaac
    20401 ttgtggatgt gaccggcctg ccggtcggca tggaggtcaa gtgcccatac accggaaagc
    20461 tcttccgcgt gccgccgcaa taaatttcac cgtgtgtgat tgttttgcgc cctgtcctgc
    20521 cgaagcggga cagggctttt tgcattctga aacaatgcac gggttcccat tcacgtttga
    20581 ttgtccttat gcgatttctt atcttaagtc ttctctgcct gatgacgacc ggtttgtccg
    20641 ggcagggcaa agaactcacc ggcacctacc tgccctcgac gaacgcgtgt ccctcgccgg
    20701 aggaggcgag gaagaaaatg accgtgccgg agggctacga ggtccgctgc ttcgcccatg
    20761 agccgatggt gcaaaatccc gtcgccatga cctgggatca tcgcggccgt ttgtgggtcg
    20821 tggaggctta cgagtatccg gaaggctcca aacatcccgc gcccttcggc ggcgaagcta
    20881 aggacgatcg ataccatcca atgccaggcc gcggcggcga aatcccccgc gaccgcgtca
    20941 tcattctcga agacaccgac aacaacggcg aggccgacaa gcgcaccgtc ttcgtcgagg
    21001 gtctgaatct cgccagtgcg atcctttgcg gcgacaacgg catctacatc ggccagcagc
    21061 cgcacctgct gcacttccgc gatgacaacg gcgacgacaa accggacgcg tggcgtgtgg
    21121 tgctcaccgg attcggccgt gaggacacgc atgagctgct gaattccttc tgctggggcc
    21181 ccgacggctg gctctacatg acgcacggcg ttttcaccaa cagcaaggtc cgccgccccg
    21241 gccagcccga gagcgaaggc tttaagtttg atgcggggat tggtagagcg aggccggtag
    21301 gacaggcttc cagcctgtca cccagtgcgg cgggcatcct gcccgcccaa gaccagccgg
    21361 gcaagatgcc cggcacaccc gaggcaggca agatgcccgc cctaccctgg gaattcgaag
    21421 tcttcgccga cggcaccagc aatccctggg gctgcgacta cgacgccgcg ggaaacttct
    21481 tcgtcagcgc ctgcgtcatc gatcacttct tccacatggc tcccggcggc atctacgtgc
    21541 gccagggcgg tgcgccggag aatccgtatg cctacgagct gctgcccagc atcgtgaagc
    21601 acaagcactt ccgcgccgcg tatgccggcg tgcagattta tcaaggcggc cgctatcccg
    21661 ccgacacgca cggccacgcc ttcatcggca acatccacga caacgccatc cacgaggaag
    21721 tcctcacgcc cgtcggcgcc accttcaaat gcgagccgcg tcgcgacttc ctccgcgcca
    21781 acgacggctg gttccgcccc gtcagcaccc agaccggccc cgacggcttc ctctggatca
    21841 tggactggtg cgacaagtat ccctgctacc aaaacgccaa agccaacccc gaaggcgtgg
    21901 accgccaata cggccgcatc tggcgtgtgg tgtacaagag cgaagttgga gtccaggctt
    21961 cagccgacaa aaacgccctc gctccgccca gccatcccac ctcgcctaaa ggctccactc
    22021 caacgcggcc cacgaacgac ctcgacctca aaaaactctc caccgccgat ctcgtcaaga
    22081 ccctggagca tcccaacaac tggatgcgaa ggatggcgag gcgcatgctg gtggagtcag
    22141 ttggcgagca agatcgttca ttgctttctt tgttgaagga caacatgccc aacagcgccg
    22201 attatgcagc tattgaagtg ttttggactg cccttgccag cggtataatt gattttgatt
    22261 tgataaaagc agctttgtcc agcaatcgca acgatctcaa tcattgggca gccagagctt
    22321 ttggtgagca tgagcccaat gctcccaaat tgcttgttgg actggccgaa caccaagagc
    22381 cttttgttcg gctcgcggcg gcagttgctc tccggcaata catatctgga tctcttacag
    22441 tgaatcatcg ccgcaaagtg cctgttcaag accatgctga cattctgatt gcaactcgtt
    22501 ccgccttgat ccgttcatcg tctgatgccg atcctgtcct agctcatgca atctggatgc
    22561 tgattgagcc agcagcagat tcattcagat ccagcgccct cggcgaatca cggaatgctt
    22621 tcctggaaga aatggcttct ggtgccgctc aatcccagcc cctctcccgc gtcctctccc
    22681 aaaaagccat gcgccgcttg tgcgacacgc ggaaggcgga gaacctcgat ctcgcggtcg
    22741 agttctgcga caaaatcgcc gagcacgaca tcctgctcgc gcatgcgctg gatggactcg
    22801 tcagaggtca ggaaggcggc gtgatcaagc cggtgaagga ggtctcggcc agcctcgcga
    22861 agtggcgttc atcctcgaat gcggacgtgc gcaagcatgc gcagacactc gcagtgatgt
    22921 ggggcgatgc ggcggcggtg aaggaagtga tcgcggtgct gcacgatggc gcgaagtcgg
    22981 agaaggagcg catcacggtg ctgcaatcgc tgcaaaaggt gcgcacggac gcggtgaagg
    23041 acggtttgaa gccgctgctc acgccgcaga cctccaccgc actcgccgtg gcggcgatac
    23101 gcgcggcggc ggacctcggg ggcgatgagt tcatcgcgcc gttgcttcaa caatcagcct
    23161 cgaaggactt caacgtgcgc aacgcggcga tcgaggcgct ttccaatcgc gacacgtgga
    23221 cgaaggcact tctgagcgcg atctccgagc aaaaagtcag cgcgagcggt ttccccgcgc
    23281 cggtgcggcg tgcgctggcg acacacaagg acaaaggcat ccgcgatctc gccttcaaag
    23341 tgctcggcgc gtggcaggat tcgtccgacg atgtgaaatc gctcatcgcg cagaagaagc
    23401 aggcatgttt gacgggcgaa cccgatctgg cgaacggcaa actgctcttc acggccacct
    23461 gcatggtctg ccatgagttt cacggcggcg gacagaaggt ggggccagat ttgatcggca
    23521 gcggtcgcag caacctcgat gcgctgctgg cgaacgtcat cgatcccaac cagatcatcg
    23581 gcaacggcta cgaaaacatc agcgtgagca ccaaggacgg ccgcacgctc gcaggccgca
    23641 tcgtcgagga cacgccgggg catgtgaaac tgctcggagc cggtggagcc gcgcaagtcg
    23701 tgccacgcga tcaaatcgcc tcgctcacca acacgaagca aagcctcatg ccgatgggat
    23761 tcggcaattt gcccgatgcg gccttccgcg acctcatttg gtacattttg gctccaccgg
    23821 aagaaggccc gctcacgccc gaaaagaaga aattgctcat ccaaggcgtc gaagtgcccg
    23881 aaacggcctc acagccgaaa aagaccaact ggcgcgccat cgactgggag agcgtcagcc
    23941 tgtggaatcc cgactggaag gtctccgcgc ccgatttcga gcgcacgccg gtcaaactgg
    24001 ccgaatacca cggcaaaacg aacgtgctgc tcgtgcatcc gttcccggac aaaaagacgc
    24061 ccgcgagctt tgagcgccaa ctgaagctcg agaagaccaa actgaagttc agcgtggccg
    24121 ccgatgatcg cggcgactgg ctggccaaag ttttggtcaa cggtcaggag gtcaaggcag
    24181 tgaccgtgga ccatgagaag ccgcgctgga agaccgtgga ggtcgatttg acgaaatgga
    24241 agggccggga cgtcaccctg cgcctcgaag cccacgcgaa cggctgggcc tgggagttcg
    24301 cctactggca tgggatcacg ctcgagtgag cttgccaacg gaaccgttgc ttgggcagtc
    24361 gcgtcttttt caagaatgaa acgcctgccg aagagcaacg agtctgttgc attgggtatc
    24421 gttcgcgcta gcgtctccgc tgcgataaag tctcaatagc aagcatgccc ctcactctcg
    24481 cccaactccc tgttggccag caggccgtca tccactccat gcccacaggg cgcgcgggcc
    24541 tcacccggct gcgcgagttg ggcctgactc cgggagcgcg ggtgcgcatg gtgcggcgcg
    24601 cgcctttggg cgagcccatc gagatcaccg ttcgcggttc ccacatcgcc atgcgcaacc
    24661 acgaagcggc ggacatcgcc atcacgctgg aatgagcgac gccgcgccac ccctcctcgc
    24721 tgtggtcggc aacccgaact cgggcaagac cacgcttttc aatctgctca ccggtctgcg
    24781 ccaaaaggtg gcgaactacc ccggtgtgac ggttgaaaaa aagatcggcg aatgccgcac
    24841 ccagcatggc aaaaagctgc gtgtcattga cctgcctggc gcctacagcc tgaacgcccg
    24901 cagcccggac gaggctgtgc tgcgcgatgt gctgctgggc cgccgcgccg acacgccacg
    24961 gccggaccgc atcctctttg tggccgatgc ttcaaacctg gagcggaatc tctacctgct
    25021 ctcccaagtg ctcgagctcg gcctgcccac ggtgcttgtg ctgaacatga tggacgtggc
    25081 agcgcacaag cagtggcgca ttgacatccc gcgcctgtcc gaggagctcg gcgtgcccgt
    25141 ggtgcccatg caggccatca gcggccaggg tttcctggaa ctgcgcgtgg ccatcagtcg
    25201 ggaagacctg cccgcgccgc gctggcactc agcgcctctg ccggaggaca tccgcgccgc
    25261 tttgcaccgc agcctcgaca gtcttgccag cgccggtgcc attcatgaca aagccacgct
    25321 gctggagccg ctctacctca tggcggatca caacccgctg catgacggct tcgaggacag
    25381 tcacctggct caaatccgcg aggcgcgtga ggtgctggaa aaagcgcacc ccggctggga
    25441 ggaccagctc gtggccgacc gctacgccgc catcgaaaag ctctccgcca aagtgctgac
    25501 gctgcccgag caggaggtgc gcaccttcac gcgcaggctc gaccaatttc ttctccaccc
    25561 gtttttcggc ggcgcggtgc tgctgggcat cctggccttt cttttcttcc tcatcttccg
    25621 tgtcgctgaa ggccccatgg gctggattga ggaatccttt gccgccctgg gcaacactgt
    25681 caccggcctc atgccgcccg gtgatttgcg cgatctcatc gtggacgggg ccatcaatgg
    25741 cgtggcgggt gtggtcgttt tcctgccgca gattttgatc ctgtttttct tcatcggcat
    25801 catggaggac accggctaca tggcgcggct ggccttcatc atggaccgcc tgatgagcgt
    25861 cgtcggcctc aatggcaagg ccttcctgcc cttcctcagc agctacgcct gcgccgtgcc
    25921 cggcatcatg gccacgcgca cgattgacag cccgaaggac cgcctcatca ccatcctcat
    25981 cgccccgctg gcgagctgct cggcgcgcct gccggtgtat ctgctgctca tcagcgccct
    26041 catgccggaa agcgtgtccg cctgggcgcg cgcgggcacc atggtcggtc tctacgcgct
    26101 gggcacgctc ggcgccttcg gcttcgcctg gatcttcaac aaaggcatcc tgcgcggggc
    26161 gaacgcgccc atgatcctcg aaatgccgaa ctacaagctg ccctcgctga aggccatcct
    26221 gctccaggtg tggcagcggg ccaaggcctt cctcgtgcgc gcgggcacgg tcatttttgg
    26281 catgtccatc ctcatctggg cggcctccac ctacccgaaa gacagcgccc tgctggccga
    26341 ggcggagaaa atcgagcagc gcattgagtc ccttgaaacc tcgggggatg aagccgcgct
    26401 caccgccgcc gctgacgagc tcgccattgt caaatcccgc cagctcgcgc aaagcctcgc
    26461 cgggcgcgcc gggcacctca tcgagccgct catcgagccg ctcggttatg attggaagat
    26521 cggcatcggc cttatcggca gcttcgccgc gcgcgagctt tttgtgaaca ccatggccgt
    26581 ggtctatgcc gtgggcgcgg aggatgagga ggacatgacg ccgctgcgcg agcgcatcca
    26641 gcaggagacg cgcgcggatg gcacgccggt ttacagcccg ctggtctgcg tgagcctgct
    26701 cgtgttttac gtcttcgcca tgcagtgcgt gagcacgctc gccatcgtga ggcgcgagac
    26761 cggctcgtgg aagtgggtct ggttccagct cttctacctc agcggcaccg cctgggtgct
    26821 cgccttcgcc gtgtatcaga tcggcggcat gatggggtgg tgatgaagaa tcaaggtgac
    26881 cggaccattc cgctcgtgtg ctcgcatcca tgaccgagca cgccccttgt ggtattccag
    26941 cttgccaaaa agcgcgctgg gcgtgaaaaa cagcgcatgg aatcccacca agtcatccgt
    27001 caggcggtcg agagttcgaa cgtcaaaaag gtcgccagcc agttgggcat gtccctgtcc
    27061 cttgtttaca agtggatgga gaaggatgcg aggaatcaaa gcggcgtgct caacccgctt
    27121 gatcgcatcc accagctcta tgagatcacc gggcacgatc acctcatcca gtggctctgc
    27181 cacaaggcgg gcggctattt tgtcaaaaac ccgcccaccg gcaaacgccc gggacgcgag
    27241 gtcatgcccg ccacgcagga gatcgtgcaa aaattcgccg acgttctctc cgccatcagt
    27301 gacgccgcgg ctgacaacag catcagcgac gaggaggcga agctcatccg ccatgagtgg
    27361 gatgagttga agcggctcac cgaacgcttt gtgaaatggt gcgaggaggg cgacttcgag
    27421 cacctctccg aagaccttgc atccaaacat ctccgttaac gaccccccga tgcccgacct
    27481 gaagacctac ctcgccgagc gctgcgcctt tgtggacagc atgctcgacc gcctcatccc
    27541 ctccgcagac accgcgccgt ccaccatcca ccgcgccatg cgccacagcg tcttcgccgg
    27601 tggcaagcgc ctcaggccca tcctctgcct tgccgccgcc gaggcctgcg gcggcggcaa
    27661 ggagagcgcc gccttcgccg cctgcgcggt cgagtgcctg cacacctact cgctcatcca
    27721 tgacgacctg ccgagcatgg acaacgacga cttccgccgc ggcgtgccca cctgccacaa
    27781 ggtcttcggc gacggcatcg ccatcctcgc cggggacgcg ctccaggcgc tggcctttga
    27841 aatcctcgtc aaaacacccg tcaccgtccg ccacagcgcg ggcgctttgg tggccgaact
    27901 ggcgcgcacc gccggcagtc tgcacctcgt cggcggccag gtggcggatc tggaaggcga
    27961 ggggcaaaag ctgcccctcg aagccctgcg cttcatccat gaaaacaaaa ccgccgccct
    28021 gctcgccacc agcctcaagc tcggcggcat gagcgccgac tgccagccgg aggagctggc
    28081 cgccctgcat gacttcggca tggccgccgg cctcgccttc cagatcattg acgacatcct
    28141 cgatgtcacc cagaccagtg aaaagctcgg caaaagcgcc ggcaaggacc tggcctcgga
    28201 aaagtccacc taccccgccc tcatcggcct ggacgcctcg cgcgatgagg cgcaccgcct
    28261 cacccgggtg gcgcatgatt ccctcgccat cttcggcccg cgcggcgcgc gcctgcgcga
    28321 gctggcggac cacctgctgg cgcgtgaata ttgagcaacg tccttcaacg catggacgac
    28381 ttcctggtca tcgagtccgt gagccgcagc ttcggcaggc tgcgcgcgct ggacgatgtc
    28441 tcgctcgcca tccgtgaagg cgaggcattc tccctgctcg gccccagcgg ctgcggcaag
    28501 accacgctgc tgcgcctcat cgccggtttt gacgcgccgg acgcgggccg cattttgctc
    28561 ggcgggcggg acatcaccgc gctgccgccc gagcgccgtc cggtgaacac cgtgtttcaa
    28621 aactacgccc tctttccgca tctcagcgtg cgggaaaaca tcgccttcgg tccgcgcatg
    28681 gcaggctggc cgcgcgagcg tgtccgcgag tcggtggagc aaatgctcgc gctcgtgaag
    28741 ctcgaaagcc acgccggcca ccgccccgcc cagctcagcg gcgggcaaaa acaacgcgcc
    28801 gccctggccc gcgccctggt caaccgcccg cgcgtgctcc tgctcgatga acccctggcc
    28861 gcgctcgatc tgaagctgcg ccagcacttg ctcgtggagc ttggcgcgct gcatcgcgag
    28921 gtcggcgcca ccttcatcta tgtcacgcat gaccagtcgg aggccatgag cctgagccac
    28981 cgcatcgccg tgatgaacca gggccgcatc gagcagatcg ccgcccccga gcagctctac
    29041 gcccggccgg ccacggactt cgtcgcctcc ttcatcggcg acaccaatct cgtcgcgggc
    29101 cgcatcaccg aagatggcaa tgccgtccgt tttgccgatg gcaccctgct cccgctgccg
    29161 gagaaatccg cgcacccgcc aggcaccctc acccgctgga gcatccgccc cgagcatgtg
    29221 cgctgcgggc cccgcccccc gagtgacgag cctgcggtgg tctccgaggt catctacctc
    29281 ggcacccaca cccatgtctg gatcgagagc ggcccgctgc gctggctggc gcggatgcaa
    29341 ggcccccccg ccttccagcg cggtgcccct gtatccctca ccctcgatcc cgccggagcc
    29401 tgcgagctgc ccgccccagc ctcatgaaaa agccgccggg tgaccgcggc ggaaaaggct
    29461 cccatcaagg gccgccttca gcttcacggg acgccggcaa cacacgcggc gcgcgcccat
    29521 ccgggcgttg acgatgcagc cctgccctgg atggcaggct gccctcccgc gcagaagatg
    29581 aaattccggt gcttcatgat tccggaatgc tggcaacacg aggggttgcg gctgcttgcc
    29641 tggaagcttg ttggattcca gtttccgccg cttccgccgg tcatccagca aagtgtgatg
    29701 actcacccat ccagtgacgc aacatcaacg ctcttgagcg cttccctgcg ccacctccat
    29761 ggggcggaaa caaggctgcc acctcatcat ccacagctca caacttcctc agccggaaga
    29821 aaacacgccc gccgccggcg gcgaactgga ggaactgctc ggctcccatc accgtcggcg
    29881 tgcccgccac atcgagccag ttctcggggg cgaggtcggt gttgttggct tggagcaccc
    29941 agccggggac attcgatccc cagacgactt ggacattccc gcccacactg cggatggtga
    30001 ggtgcggcgc gccggtttgc tgcagcgcca tgtactggcc gaagaatccg ccgctgatgc
    30061 cgtagccgcc cccggtggag ggggggctgg cctcaggctg ggcgatggcg ccggtgacgg
    30121 catacgcccc gccgctgctg gtgccaccgc cgcctgctat gatgcgggag gtgatggagt
    30181 agctctgcgc gtgcgcggtg gcggtaagca gggggaaggc gaggcggagg gtgaggcgtt
    30241 tcatgtcaaa acaggctgag gatcaacgag aaaagggtgg gtggcaggtc gcttaggggc
    30301 cggctgacag gcaaaaaggg gtgccacccc ctggcgcggg gggtggcata ggcagccgcc
    30361 gggggtgcca ccccctggcg cggggggtgg cataggcagc cgccgggggt gccacc
//
LOCUS       DAOMYJ010000037        29713 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Prosthecobacter sp. isolate AMR_MDS_5629
            NODE_552_length_29713_cov_24.088239, whole genome shotgun sequence.
ACCESSION   DAOMYJ010000037 DAOMYJ010000000
VERSION     DAOMYJ010000037.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867850
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prosthecobacter sp. (wastewater metagenome)
  ORGANISM  Prosthecobacter sp.
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Verrucomicrobiaceae; Prosthecobacter.
REFERENCE   1  (bases 1 to 29713)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 29713)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.30
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:34:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,742
            CDSs (total)                      :: 4,684
            Genes (coding)                    :: 4,676
            CDSs (with protein)               :: 4,676
            Genes (RNA)                       :: 58
            tRNAs                             :: 55
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29713
                     /organism="Prosthecobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_552_length_29713_cov_24.088239"
                     /isolate="AMR_MDS_5629"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1965333"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..768)
                     /locus_tag="PLP58_05980"
     CDS             complement(<1..768)
                     /locus_tag="PLP58_05980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018970654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HRJ08097.1"
                     /translation="MRILITGGAGFLGSHLCERLLNEGHEVLCMDNFFTGRKANIAAH
                     LGNPRFELFRHDVIDPFKAEVDRIYNLACPASPPHYQHNAIKTVKTSVMGAINVLGLA
                     KRVRARVFQASTSEVYGDPEVHPQPESYRGNVNPIGLRACYDEGKRCAETLFFDYHRQ
                     NGVDIRVVRIFNTYGPRMLPDDGRVVSNFIVQALRGENITIYGDGTQTRSFCYVDDLI
                     EGFVRFMESTGIPGPVNLGNPGEFTMIELAELVLKKVG"
     gene            870..1835
                     /locus_tag="PLP58_05985"
     CDS             870..1835
                     /locus_tag="PLP58_05985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018968981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HRJ08098.1"
                     /translation="MDSSASPFRVGVAGVGAIGKNHARILAEIAARSGGAVEFAAVYD
                     ADAARAAEFAAQYQTRAASDLDDFASRVDAATVAVPTVYHRKVAEPLMLAGVHVLVEK
                     PVSESYDDAQAMIGLAQEKNVILQVGHIERFNPVLRQLEERMNQPRFIECHRLSPFPN
                     RSMDIGVVLDVMIHDIEIVLHLVKAPLVQVDAVGIPVLTKREDIANARLKFANGCIAN
                     ITASRISPEKMRKIRVFQSDCYLSLDYQEQSGWIYRRDGMQIVREAVEVERDEPLKLE
                     IAAFVECARQGKRPVVTGQEGAEAVRIALEITDQIVRNAAMAQLP"
     gene            complement(2103..4919)
                     /gene="gcvP"
                     /locus_tag="PLP58_05990"
     CDS             complement(2103..4919)
                     /gene="gcvP"
                     /locus_tag="PLP58_05990"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012419501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004375 - glycine dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase"
                     /protein_id="HRJ08099.1"
                     /translation="MSVPFSSRHIGPTAEETAAMLQSLSVDSLDTLIDQVVPESIRQR
                     DVLNLPEPLDEEAALRRLRQMMSRNKVVRSFIGLGYHDTFTPPVIQRNILENPGWYTA
                     YTPYQPEISQGRLEALLNFQTMITDLTGLDVANASLLDEGTACAEALGLAVAQVAGAN
                     RVVVSDQCHPHSIAVVRTRMGALGIRVEVVDLATWQPDGEKGLAAVLVQYPDTLGVIR
                     DFSALASAVHEAGALLVVAADLLALTLLRPPGEFGADICVGNSQRFGVPLGFGGPHAA
                     FMAVKDALKRRLPGRLIGVSKDAAGNPACRLSLQTREQHIRREKATSNICTAQVLLAV
                     MASMYAVYHGPEGLKKIALRVHGAAVWLARELLEAGLDIASDEFFDTITVRVRSADEV
                     LKSAFLYGINLRRVDDRLVGVALDERVRDDDLATILKCFGIPKARHSREDAAEPHFST
                     VHHRASACLTHPVFHKHRSETEMMRYLRRLQDKDIALDRSMIPLGSCTMKLNAAAEMM
                     PLSWPEVNGIHPFAPPEQTRGWQDLFAELEQWLAECTGFDATSLQPNAGSQGEYAGLL
                     AIKRFHESRGEGARDVCLIPASAHGTNPASAVMCGFKVVPVACDEMGNISLGDLRARL
                     AANEGRTAALMVTYPSTHGVFEEGIAEICALIHEHGGQVYMDGANMNAQVGLTSPGRI
                     GADVCHLNLHKTFCIPHGGGGPGVGPICVAAHLKPFLPGHLTWPEQSEGAVCSAPWGS
                     ASICTISWMYIAMMGPRLTEATRFAILNANYISHRLAACFPTLYRGGSGHVAHECILD
                     FRGFHKVTVEDVAKRLMDFGYHAPTMSWPVGGTLMIEPTESESKAELDRFCEAMEQIH
                     REIAAVESGAADALDNPLKRAPHTAADVTKTDWQHSYTREAAAFPLPWVRESKYWPPV
                     ARVDNVHGDRHLICTCVSVEEAAE"
     gene            complement(5090..6274)
                     /locus_tag="PLP58_05995"
     CDS             complement(5090..6274)
                     /locus_tag="PLP58_05995"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018969020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HRJ08100.1"
                     /translation="MLEKKIRLEHSHRILFTRQAFAPANPCIRDLLLLDSPKKPPRVL
                     VFADDQVLAANPGLRESIRAHARAHAEVYDLAGEVVELPGGESCKNDFALVEDCWEAI
                     HEAGLDRHSYVFVIGGGAVLDLVCFAASTAHRGIRHARFPTTTLSQGDGGVGVKNGVN
                     FFGKKNWVGSFAVPYAVVNDFAFLESLPARERRNGIIEAIKVSLIRDRAFFEEIERLA
                     PELARLEQPALERVVQRSAELHVDHIATGGDPFELGSARPLDFGHWAAHKLEQITRFE
                     VTHGEAVAIGMAVDLVYSVKTGLLDEATAQRVLRLVESLGFHTYHHGLLAEGQRGGPV
                     ILDGLEEFREHLGGELTVTLVPEVGRKLEVHEMDPSVIVAAMEELKARSSKFEIRNTG
                     LR"
     gene            complement(6368..7261)
                     /locus_tag="PLP58_06000"
     CDS             complement(6368..7261)
                     /locus_tag="PLP58_06000"
                     /inference="COORDINATES: protein motif:HMM:NF013226.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UbiA family prenyltransferase"
                     /protein_id="HRJ08101.1"
                     /translation="MLRAWLELARISNLPTVWTNVSAGWLLAGGGWDARLGWLLLAGS
                     LLYTGGMILNDAADVKFDREHRPERAIPSGRVSAEAAWAVGAGMLAAGAGMAVAQGGA
                     CPWIAAGLVASVILYDLFHKPWSGAVWVMGSCRLLLVLLAASANDTGCKMSGEMLATP
                     HGIALGVYIVGLTKVARLESSKEGAVVLSLVMAKLLLWVPAILAVAFTFTFFWVAVDA
                     EPSDWLPLLMLPVFAIWVFLSMRIMRKGGPHVGRAVGWLLAGIPIVDALAVGHVSLTL
                     ACGFAALAPLLRLWQRWIAAT"
     gene            7391..7906
                     /locus_tag="PLP58_06005"
     CDS             7391..7906
                     /locus_tag="PLP58_06005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009964548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HRJ08102.1"
                     /translation="MDDATTAFLELLTKHERPLSLYVHGLVPRDSAAEDILQQTKMLL
                     WKHFKDFEPGTNFLAWARKTAFHQILTHRRQKKREHLPLDEVALEALGHAVSDLAVEG
                     SARQDALRACMARLPVEHRRLISLRYFEEMEIGQIAGRIRRSEMAVYRALSRIRMTLM
                     QCIEKQMEAHS"
     gene            7903..9312
                     /locus_tag="PLP58_06010"
     CDS             7903..9312
                     /locus_tag="PLP58_06010"
                     /inference="COORDINATES: protein motif:HMM:NF033679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNRLRE domain-containing protein"
                     /protein_id="HRJ08103.1"
                     /translation="MKAEETWRWLELWERARDRALTHEEEALLNEAVTCNPEVRALLA
                     RAALMDAETACMEMPETISAPSPRTVSVWRRALLPAAAALVSAGLVWLWDREPPPVAT
                     LVRAAACKWGNSALPTLEGSALRPGLLELLDGIATLKFKSGAEVTLEAPVTLEVLSTM
                     ECRVKKGTVMAEVPPSAKGFTIHTPETKVVDYGTRFGVSASEDGKCLVHVMEGLVEVE
                     RAGEKGRKELRAGERADYGGFIKQALNPDADPFEQTESSRWLPGPINQLADGWQLVTT
                     AYGRGRDSWVQSSKQKVSGRESFLRVKFTTLNDGLNRKAYAAFDLSAFAGKRVAEAEF
                     ALHVEPSDLGFASLVPDAVFTVHALADEAQDAWDEAELSWENAPAHSTAPEHRNSPVA
                     AQSVLIGEFTVPQGTTRGAFSVKGAALVRSLNADTNGMLTLIICRQTDETATSGLAHA
                     FASKENTRNTPPLLKVRLE"
     gene            9405..10373
                     /gene="trpS"
                     /locus_tag="PLP58_06015"
     CDS             9405..10373
                     /gene="trpS"
                     /locus_tag="PLP58_06015"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009965495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004830 - tryptophan-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006436 - tryptophanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HRJ08104.1"
                     /translation="MRILSGLQPSGRLHIGNYFGMMEAALKLQHEGEAFYFIADYHSL
                     TSIQDGKLLRSHVRDLAIDFLACGLDPAKCVLFRQSDVPEHTELAWILSTVTPMGLLE
                     RCHSYKDKIANGISPSHGLFAYPVLMAADILIYDSDVVPVGRDQKQHVEVTRDIAVKM
                     NMAFGEGLLKVPEPRIREETAVVPGLDGRKMSKSYNNTIQLFEEPAALKKKIMRIVMD
                     STPLEAPKPIENNSVLALYKLVASEADYQTMVDDHLRGGIGYGEFKKRLLEAMLAHFA
                     PFRERRAGIVADPAYVNRVLAEGAEKARAIARQTLNRVRAAVGLGD"
     gene            10654..11508
                     /locus_tag="PLP58_06020"
     CDS             10654..11508
                     /locus_tag="PLP58_06020"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF022137.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HRJ08105.1"
                     /translation="MQDGYELLDSGEFQKLERFGSVVLSRPCAQAVWRQSLSKADWQR
                     ATAAFFRDGGNQWRGRDRLPATWEISVDGMRFQLSSTDFGHLGIFPEQRDQWRRIREL
                     CAAYPKAHQRAPRVLNLFAYSGGSTLAAAHAGAEVCHVDASKGMVDWARKNAALNGLE
                     DRPVRWIVDDVTKFLERELRRSRSYDLVILDPPSYGRGAKGEVFKIENDLPPLLALIG
                     QLMSSQPLGVLLSCHTPELTQTSLHHLLLQEFGSKGGALESGEMLLRGGKDVLPVPSG
                     SFCWWVRA"
     gene            11674..12930
                     /locus_tag="PLP58_06025"
     CDS             11674..12930
                     /locus_tag="PLP58_06025"
                     /inference="COORDINATES: protein motif:HMM:NF014691.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HRJ08106.1"
                     /translation="MRSEDLPQHEEYLHWDKLRHLSPPAGLSHEEWWWLVKRGRQSGR
                     QGIPLRDKKGVSFSFSLVPEMFEALHHIDQRCAGNIAMPEQVTDGRTRDRYQINSLME
                     EAVTSSLLEGAAVTRQKARDMLRSGRKPTDKSERMIVNNFKTMQHLLNIKDRPLTPEL
                     VFEIHAMISEDALDKPDGAGRLRRADEYIEVSDDLDNIFHIPPAASQLPGRLEEMCFF
                     ANETGVNGFLHPVIRSIALHFWLAYDHPFVDGNGRTARALFYWSMLRGGYWLFQFISI
                     SQALLRSPSDYYRAFLHTKHDENDLNYFILHQLRCIETSIQDLHEYLARKSEQRRGLQ
                     IILRGLDWVNHRQQALLSHALEHDDAAYTFESHQRSHRISRMTAISDLRSLEEKGLLV
                     SRLQGRQRVFRPIPNLETKLGTRTRP"
     gene            13102..13566
                     /locus_tag="PLP58_06030"
     CDS             13102..13566
                     /locus_tag="PLP58_06030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="group III truncated hemoglobin"
                     /protein_id="HRJ08107.1"
                     /translation="MLESKPDIQGRAEIETLVNTFYERVRADDLLGFIFDKVARTNWE
                     SHLPKMCAFWETVLFRSGGYTGNPLAAHAKLVPRAAMGREQFDRWLMLFRSTVDDLFA
                     GENAGHIKNCAADMANVIYARINQVADPRFDPANLTAEQKERYARYKASAST"
     gene            13563..14765
                     /locus_tag="PLP58_06035"
     CDS             13563..14765
                     /locus_tag="PLP58_06035"
                     /inference="COORDINATES: protein motif:HMM:NF017727.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NnrS family protein"
                     /protein_id="HRJ08108.1"
                     /translation="MSQFPACKHKTHKAKGPRKDGLRWLAAEPYRVFFASGAVWSIIG
                     VLLWPLYYAQMLGFYPNLVHARLMIEAFGGAFVVGFLGTAGPRMATAPKLTPAELYWL
                     FALHQACGVSHLTLHFAWGDGFFIALLVSLVLCLVIRVARFRKEMPPPQMLLALTGLV
                     CGIAGAVMHLSPATLMDPQRLRLANLLLYQGLLLPPVLGIGSFVFPRMLGGDFGDPKT
                     AAQSKAKLLRAIAVAALLVGSFFLEAHGGVATGHSVRAIAAAGYLLIEVTWQTRQSGS
                     LTTGLFWALATGWLGIVLAPFYYLQHVSIEHLLYIGGFGMLMLIVGSRVLFGHSGDLE
                     GFFAKSKWARFLIFLGILAATTRATPAWVPSTTISHHIYAAWTWGLLALLWLLWHRRR
                     FVRRDGEP"
     gene            14844..15827
                     /locus_tag="PLP58_06040"
     CDS             14844..15827
                     /locus_tag="PLP58_06040"
                     /inference="COORDINATES: protein motif:HMM:NF012770.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HRJ08109.1"
                     /translation="MPVHPSAYQAPFFLRRGHAQTILGALTPAWTRPVFSPETLPLPD
                     GDCLHLGWLRGGHARLVILSHGLEGSMRAAYVRGMAAACARAGWDVLAWDYRGCGGRA
                     NLLPRSYHSGESGDLRHLITHAAPGYDSVALVGFSLGGNITLKYLGESPPHPAVRAAA
                     AVSTPVDLASSARALDEHPACRLYQRRFLRSLLAKAEEKARRFPRVLPPGGLAGIRTI
                     RDFDERFTAPLHGFRDAADYYARASSLPHLNSITVPALLLNAADDPLLEPPSYPGGAA
                     AENAALHLEIPAHGGHVGFLTHGLRRWHERRVLDFLANSSPSKPTHSRLHA"
     gene            complement(15846..15930)
                     /locus_tag="PLP58_06045"
     tRNA            complement(15846..15930)
                     /locus_tag="PLP58_06045"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(15895..15897),aa:Leu,seq:taa)
     gene            16155..16469
                     /gene="rplU"
                     /locus_tag="PLP58_06050"
     CDS             16155..16469
                     /gene="rplU"
                     /locus_tag="PLP58_06050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009964411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HRJ08110.1"
                     /translation="MAYAIFKTGGKQYKASVGDQLDVEKIEVAEGEAANFDQVLAAGE
                     GADIKVGAPVLEGASVGFKVLKQFKAPKVTAFKFRKRKGYHKTKGHRQFLTRVEVTSI
                     NA"
     gene            16504..16761
                     /gene="rpmA"
                     /locus_tag="PLP58_06055"
     CDS             16504..16761
                     /gene="rpmA"
                     /locus_tag="PLP58_06055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018969714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HRJ08111.1"
                     /translation="MAHKKGQGSVKNGRDSNSKRLGVKKYGGESVIPGNILIRQRGTK
                     WTAGSNVGMGRDHTLFALVEGKVRFDKDGRRINVDEAVLQA"
     gene            16815..17528
                     /locus_tag="PLP58_06060"
     CDS             16815..17528
                     /locus_tag="PLP58_06060"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HRJ08112.1"
                     /translation="MNRTGLARALSKLGFCSRSQGFELIRAGQVRLNGRICMNPEQPV
                     LLDKDDVRVDGASLAAAAPVYLMLNKPRGLVTTARDEQGRDTIFSLLKGAGLPHLGPV
                     GRLDKASEGLLLLTNDTSWAHRVTDPASHLPKTYHVQTAGLLGEAALTRFGAGIRDDG
                     ERLRLPDARVLRQGEKNTWLEIVLTEGRNRHIRRVLEAFGIEVLRLVRVAVGPLVLGE
                     LAKGGWRHLTTQEVSILEN"
     gene            17643..18062
                     /locus_tag="PLP58_06065"
     CDS             17643..18062
                     /locus_tag="PLP58_06065"
                     /inference="COORDINATES: protein motif:HMM:NF019262.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ08113.1"
                     /translation="MKSLSRFTLAAAFLLAGTGHAADARADFATQIKPVLGTRCINCH
                     HSGALFGELNLENREMAFKKRANGPVIIPGKPDSSMLYLVLKLPPKELKAMPPNGHRI
                     PDREVNLIHDWIRQGADWPAGAEGVIQPPRMKTASGS"
     gene            18089..19732
                     /locus_tag="PLP58_06070"
     CDS             18089..19732
                     /locus_tag="PLP58_06070"
                     /inference="COORDINATES: protein motif:HMM:NF012706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HRJ08114.1"
                     /translation="MTAWITRLVLLSGLIAALVFFHHSRAHRPPRVDAAREKGILLVG
                     NGSDPATLDPHLATGTPEHAILDSIFEGLVAATPEDPDANGHGAAERWETEDFVHWTF
                     HLRKNARWSDGAPVTARDFLFAFERILTPALGADYAPMLYPLRNAEEFNTGKIPGFQE
                     VGASAPDDHTLKLVLKGPAPYLLSMLKHYAWYPVPRHVIERHGKMTDRVSPWTRPGNL
                     VGNGAFQLREWRLTHYVSVERNPHYWDAGSVRLNGIHFFPIVSDSTEERAFRDGQLHT
                     TLTLPLAKIPLYQRQHPDLLRVDPILSVYFYRCNVTRKPLDDARVRRALAQAVDRSAI
                     VENILRAGQKPAHGLTPPGSSPDYTTPDVLRFDPEKARALLAEAGYPDGRGFPRFEIL
                     INTNEAHRSIAEAVQEMWKKHLKISIGVRNEDWGVYLDSQRKLDYSVCRAAWQGDYLD
                     PFTFLSMWKTGDGNNNTGWGSPAYDALMDASSREPDTTRRMAQLQEAERLLLDELPML
                     PLYWYVHTWLKRPEVQGWPPSLLDHRCYKAIHFQESGRR"
     gene            19954..20463
                     /locus_tag="PLP58_06075"
     CDS             19954..20463
                     /locus_tag="PLP58_06075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ08115.1"
                     /translation="MADFFTNPLYTFGMGFALGCVFLVMAVFSHWKTKGEFRRYKSHL
                     SDKLELDARQLQETNKERARLAQENENLRIQVNRLNEKPDNKLQRELEIMARAEKHML
                     VNAPGFAPAWEMAKSHAMSQLETEEKGLSFPQKIFRKLIGPGAPSNNSGPAHEPASHH
                     AETAKSDAA"
     gene            complement(20726..29659)
                     /locus_tag="PLP58_06080"
     CDS             complement(20726..29659)
                     /locus_tag="PLP58_06080"
                     /inference="COORDINATES: protein motif:HMM:NF024353.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter-associated beta strand
                     repeat-containing protein"
                     /protein_id="HRJ08116.1"
                     /translation="MLRLAGSGVLSGANATTNIRQGAVLDLNGVGTGTSIGQFNNNGV
                     VTNSSATSATIQLGNNNGTGTSFGSIQDGAGAIHVTKVGNGAQSWLGLSTYSGATTIG
                     GTGLVTVDVLADGGLASGIGASSNDASNLVFDGSTGGLSYRGNLVNGILNLGSTSAST
                     NRLFTVSGSGVTLGSTPTTNLNNAIIWSNDGAIVHGTNADRTITFTGTSQGFNTFNPQ
                     ITDSTGFATSVTKVGTGIWRLGNSANTYSGATMITQGILMAVDGQGLSSGSNLVFDGG
                     ALYSSGALNRNIGTGLGEMRFAAPAANTAQFAGGFLGGDEKLTVNWAGTPEWGVTAGF
                     LDTRNGLILNGSQARAQGATGSIALSEVEIAGDFSLGTAAGATFGPGHSYTLAQNSAT
                     VTVASTAGLAVGQSFTGTNVPSGAYIVSILSATQFQMSANTANSSGIAGNYTNGEIND
                     GHLRAIRVDDNGNTGADFATISGAISAGDAVTGIRKVGVGVLRLTGGNVYSGETNVNQ
                     GTLAVLSLGHSGDAASTPTSVGVSGVAFDNTNAITLGNGGTGAGILQYLGAGETSDRK
                     IRFDTTTGSTQIHADGSGALILTNVANDVITATGNKTLFLRGTNAEGNKITSQLSDNG
                     GTLGVTVDGGATWILTNPANNYTGLTTAGGGALGIADPGAIGSGALRLANGSVFAFGG
                     DMTITNAVEQTNNTTGAFIGDHSLTFTNAYALLSTTSNPSVTTNNILPGGVLTFGGVT
                     ADSITANRTWTIEGNGETVINGDITTSTAFGLSIVKTGNGVLQLNGAGSNFNQNNTNL
                     DIDRGTVRSGANEIIPHGAGFGNLIISPELANGDTATLDLNGFNETVNGLTSTTDGTA
                     VIDNTASGDATLTFGANDAAVNFGTGAGIYSITDTGSGKLSLVKTGAGVANLGGGSGT
                     TTLDYSGTTAIEAGTLNASAPLTATTGITVAGGATLNLYNSTAENLTGLNTLSLGAGA
                     GMTILGMELGAITSASDRLAAVGAATTANTIQINIVGLAGFGSAASYDLISAGSGLSG
                     AAYELNHMPGGFTYSLTTSDTLVQLGLAAIAAGDLYWQGDLSGSWAQLSGSNSNFSTD
                     AAGTLDSGGSPGSANTVIFSSTAASGPSLATTLDNHYFINDLKFASAPAGVTSVSIAP
                     GFSSAFGLTLAPSASTDGIDVGDNAGAVAISAPVLLGAEQTWTVAGTGANGSSLLVSG
                     ALSGAFNLTKAGDGVLTLSANNDLYLGTTTISAGTLTIGAGAGAGSIGAGNVVNNGAL
                     VYNRTGTFTVSNLISGTGTLTKSASGVMILNNDANSFAGDVTITAGTLAFTTVSNIGG
                     PASSLGQGGAIHMTGGTLRFDGDSVSQTTDRPVFTSTANSTLSLNGTNGASMTYAGAI
                     TVGIDTSGNRLILAGTAGSAGAITGGFTMTGTAADALVSGGTWTLSGMQNTVADNMTV
                     QGTSTILNLGSTGVLRFLSGAASGDLNINNGSTVNIGADNAVDMTIPYRLFIGQSAGG
                     EPGILNLGAFDLESGRFILGERAADRSGIVNGTGTLTVLLGDIDLYAGEINANFASTG
                     TATLEKFGPGTVTFRGDNSGLAATGRTILNEGLLVLDYSLSNTTKLREGSQLEMIGGD
                     LTLTGNGSASTSQTVASFSLDGSATADNGGANRVTLSPGSGQEIVLNLGAITRTNANR
                     DGTVRFVLPSGVQSDTHGVTTTTGLTNGLLGSSGFATVEDGTGTWFATKSGNNIVALV
                     SSAKNDLSIWAAGDHITDETTGFSGTLSRAAINSLRFDASGGSNLGVSPLGALHIASG
                     GLLVTSSVDAGESSVFGGNLTSGTSEIIVTQDSGRVFEISSSIITNHAFTKSGEGVVL
                     LSGYNTYTDETEILQGTLVAGGGNAIGDTSIVTLALNRHSILQLAADETIGRLSGGKR
                     ATNSEYGTVAVGGHTLTLNQSANTTYAGVFTGSGSIVMNTGSTGNLNLQNNSSGFTGE
                     VVINGGTFILSNIGQNNATTFTINKGGNLLMDNNGTTRIATRILDTSEIILNSADGGF
                     SGATINLPRGLGMRTDQESTNTEVVGVVTANSGASYVTIEATTANDDPDLRMSDLVRN
                     NNATINVRGTVLGLATANVQRSQFEITGASEAVFMANPANLAGGGGTAGGATLDIVPW
                     AIGESLAGALGNTNMGNSLLTYAAGSGFRPLDFSTEYAAFSTAGDTNNTRESLSANLT
                     GVGGRVLNSLVVNNEAVTGLEFTGSGAGQVLANTSGAFLFTLTGGADSTAYNTLFGGF
                     DDGITVGGSEYVIHVINPSAAANAASLVATLASPLTSTADITKSGRGTLVLSAANTAG
                     GGASKTTLNEGVLEITGLANIGGNSGGLVFAGGVLRLGAGFTDDISQRSILFLEGGGV
                     MDTNGSDLALAGTLGSGPGGFTKTGLGNLTLNADATLTGPTTVAGGTLTLGNSNSIGS
                     GNLAILGGAVLDVGASEVTVGRVTTSGESPVINGTGTIYSTQGFAFGHTGDTLVGAAL
                     AGPVGLTKTQNNVLALSGASTYTGITEIQAGTLSIDSMGNVAGGSSALGNPLNARQGI
                     IRMGLTTGATTLAYTGAGHASDRLVGLQGTTGGVTLSADGTGAFELGGAVMMRGGNKT
                     LTLRGTSDPSVENSIGALDGGTLGILSVTKSDANTWVLNGANTHTGNTTAGGGVLRAA
                     SNSAFGAGVVTLVNTDSVLEIADGIAIANDLTVSNTGNNKTLRLHGGASAGVYSGVVT
                     IAETTAGNFDVGADTGGTLTLSGVIGGAVAGGLEKVGAGTVVLSNANTYTGITTVGAG
                     TLLATNTTGSATGTGAVVVAPGATLGGNGFLAPAADNSVMVDGTLQIGTAGDVSARQL
                     TITTTGAGLVTVNDIVAFDLFSGQGSGGLNDATHNDLLVVNGSSGFTIGANAQLHVTT
                     SLPIDESWVVGTEWRLFDWTGLTGGVTGAFSNLSDPAPFNYMNLPDLSSIGLAWDVSN
                     LYTQGTIMVMVPEPGRVLLLFLGLMGLHFRRRR"
BASE COUNT         5390 a         9586 c         9232 g         5505 t
ORIGIN      
        1 gccgactttt ttaagcacca gttccgccag ctcgatcatg gtgaactcgc cggggttgcc
       61 caggttcacg gggccgggga tgccggtgct ttccatgaag cggacgaagc cctcgatgag
      121 gtcgtccaca tagcagaaac tgcgcgtctg tgtgccgtct ccgtagatgg tgatgttctc
      181 gccacgaagg gcctgtacga tgaagttgga gacgacacgg ccatcatcgg gcagcatgcg
      241 cgggccgtaa gtgttgaaga ttctcaccac gcggatgtcc acgccgttct ggcggtggta
      301 gtcgaagaag agcgtctcgg cgcagcgctt gccctcgtca tagcatgcgc gcaggccgat
      361 ggggttgaca ttgccgcgat acgactccgg ttggggatgc acctcggggt cgccatagac
      421 ctcggaggtg gaagcctgga aaacccgcgc gcgcactcgc ttggcgagac cgagcacgtt
      481 gatggcaccc atgacggagg ttttgacggt cttgatggcg ttgtgctggt agtgcggcgg
      541 cgaagccggg catgccaggt tgtaaatgcg gtccacctcg gccttgaagg ggtcaatgac
      601 atcgtggcgg aagagctcga agcgcgggtt gccaaggtgc gcggcgatgt tcgccttcct
      661 gccggtgaaa aagttgtcca tgcacaggac ttcgtggcct tcgttgagca ggcgttcgca
      721 gagatgtgag ccgaggaaac cggcgccgcc ggtgatgaga atgcgcatgg cacgacgcta
      781 gtccgggacg atgcgctggc aaggacagcg tcgcgcgcgg gctttgtttc tcttgccagg
      841 gcgcaaggct tcttcaatgc tgccggttca tggattcctc agcttccccc ttccgcgtcg
      901 gcgtggccgg cgtcggagcc atcggcaaaa accacgcgcg catcctcgcg gaaatcgccg
      961 cgcgcagcgg cggagcagtg gagtttgcgg ctgtgtatga cgcggatgcc gcccgtgccg
     1021 ccgagtttgc cgcgcaatac caaacccgcg ccgcatccga tctggatgac tttgccagcc
     1081 gggtggatgc cgccaccgtc gcggtgccca ccgtttatca ccgcaaggtg gcggagccgc
     1141 tcatgttggc cggcgtgcat gtgctggtgg agaaacccgt ctccgagtcc tatgacgacg
     1201 cgcaggccat gatcgggctg gcccaggaaa aaaacgtcat cctccaggtg ggtcacatcg
     1261 agcgcttcaa ccccgtgctg cgccagcttg aggagcgcat gaaccagccg cgcttcatcg
     1321 agtgccaccg gctctcgccc ttcccgaacc gcagcatgga catcggcgtg gtgcttgatg
     1381 tcatgatcca cgacatcgag atcgtgctgc atctggtgaa ggcgccgctc gtccaggtgg
     1441 acgccgtcgg catccccgtg ctgacgaagc gcgaggacat tgccaacgcg cggctgaaat
     1501 ttgccaacgg ctgcatcgcc aacatcaccg ccagccgcat cagcccggag aagatgcgga
     1561 aaatccgcgt ctttcaaagc gactgctacc tcagcctcga ctaccaggag cagagcggct
     1621 ggatttaccg cagggacggc atgcagatcg tgcgcgaggc cgtggaggtg gagagggacg
     1681 agccgctgaa gctggagatc gccgcctttg tggagtgcgc ccgccagggc aagcgccccg
     1741 tcgtcaccgg ccaggagggc gcggaggctg tgcgcatcgc cctggagatc accgaccaga
     1801 tcgttaggaa cgctgccatg gcgcaactgc catgaaagaa ggaacggacg tcattgcgcc
     1861 gaccaaaatt ctgggggcag catgatatga acgccactgc ctgcgggctg gcaaatcgca
     1921 gtggagtcac tttgcattgc ggtcttccaa gaattcggga aggtcaggcg cagccgcttt
     1981 gtttgaatgg gcggcgaagc ccgcagcgac ctttcgcatg aataccagct tcacagcttt
     2041 ttacggtgat tacgggactg gactggactc gtctcgccat gaggaatgcc gcgagctgaa
     2101 tattattccg ccgcttcctc cacgctgacg caggtgcaga tcagatggcg gtcgccgtgg
     2161 acgttgtcca cgcgtgccac cgggggccag tatttcgact cgcgcaccca gggcagggga
     2221 aaggcggcgg cctcgcgggt gtaggagtgc tgccagtcgg tcttggtgac atcggcggcg
     2281 gtgtgcgggg cgcgtttgag gggattgtcc agcgcgtcgg cagcgccgct ctccacggcg
     2341 gcgatctcgc ggtggatctg ctccatggcc tcgcaaaagc ggtccagctc ggctttgctc
     2401 tcgctctcgg tgggctcgat catcagcgtg ccgcccacgg gccagctcat ggtgggcgcg
     2461 tggtagccga agtccatgag gcgcttcgcc acgtcctcga ccgtcacttt gtgaaagccg
     2521 cggaagtcga ggatgcactc gtgcgccacg tgcccgctgc cgccgcggta gagcgtgggg
     2581 aaacacgcgg ccaggcggtg cgagatgtag ttcgcattga gaatggcaaa gcgcgtggcc
     2641 tcggtgaggc gcgggcccat catggcgatg tacatccagg agatggtgca gatgctggcg
     2701 ctgccccagg gcgcggagca gacggcgccc tcgctttgct ccggccaggt gagatgaccg
     2761 ggcaggaaag gtttcaaatg cgcggccaca cagatggggc cgacaccagg tccgccgccg
     2821 ccgtgcggga tgcagaacgt cttgtgcaga ttcaagtggc acacgtccgc gccgatgcgc
     2881 cccggcgagg tgaggccgac ctgggcgttc atgttcgccc cgtccatata cacctgcccg
     2941 ccgtgctcat ggatgagcgc gcagatttcg gcgatgccct cttcgaagac gccgtgcgtg
     3001 gagggatagg tgaccatgag cgcggcggtc ctgccttcgt tggccgcgag gcgggcgcgc
     3061 aggtcgccga ggctgatgtt gcccatctcg tcacaggcca cgggcacgac cttgaagccg
     3121 cacatgacgg cgctggcggg gttggtgccg tgcgcgctgg cggggatgag gcacacatcg
     3181 cgcgcgcctt cgccgcggct ttcgtggaag cgcttgatgg ccagcaagcc ggcgtattcg
     3241 ccctgtgatc ccgcgttggg ctggagggag gtggcgtcga agccggtgca ttcggccagc
     3301 cattgctcca gttcggcgaa caagtcctgc cagccgcgtg tctgctccgg cggagcgaag
     3361 gggtgtatcc cgttcacctc gggccaggag agcggcatca tctcggcggc tgcgttcagt
     3421 ttcatggtgc aggagccgag cgggatcatg ctgcggtcga gcgcgatgtc cttgtcctgc
     3481 aaccgccgca ggtagcgcat catctccgtc tcggagcggt gcttgtggaa gacgggatgc
     3541 gtgaggcagg cgctggcgcg gtgatggacg gtggaaaagt gcggctcggc ggcgtcctcg
     3601 cgcgaatgcc gggccttggg gatgccgaaa catttgagga tggtggcgag gtcgtcgtcc
     3661 ctcacgcgct cgtcgagcgc cacaccgacc aggcggtcgt ccaccctgcg caggttgatg
     3721 ccgtagagga atgcgctctt gagcacttcg tccgcgctgc gcacgcgcac ggtgatggtg
     3781 tcgaaaaatt cgtcgctggc gatgtcgaga ccggcctcca gcagctcgcg cgccagccag
     3841 acagcagcgc catgcacgcg cagggcgatt tttttcagcc cctccggccc gtgatacacg
     3901 gcatacatgg aggccatgac ggcgagcagc acctgtgcgg tgcagatgtt gctggtggcc
     3961 ttctcacggc ggatgtgctg ctcgcgcgtt tgcagggaga ggcggcaggc ggggttgccg
     4021 gcggcgtctt tggacacacc gatgaggcgg ccgggcaagc ggcgtttgag agcgtctttg
     4081 accgccatga aggcggcgtg cggaccgcca aaaccgagcg gcacgccgaa gcgctggctg
     4141 ttccccacac agatgtccgc gccgaactct cccggcgggc gcagcagggt gagcgcgagc
     4201 aaatccgccg ccaccacgag cagcgcgccc gcctcatgca cggcggaggc cagcgccgag
     4261 aagtcacgga tcacgccgag tgtgtcgggg tattgcacga gcacggcggc caagcctttt
     4321 tcgccatccg gctgccaggt ggcgaggtcc accacctcga cacggatgcc cagcgcgccc
     4381 atgcgcgtgc gcaccacggc gatgctgtgc gggtggcatt gatccgagac gaccacgcgg
     4441 ttcgcgcccg cgacctgggc caccgcgagg ccgagcgcct cggcgcaggc ggtgccctca
     4501 tcgagcaggg aggcattggc cacgtccagc ccggtgaggt cggtgatcat ggtttggaag
     4561 ttcagcagcg cctccagccg gccctggctg atctcgggct ggtagggggt gtaggcggtg
     4621 taccagccgg gattttccag gatgttccgc tggatgaccg gcggcgtgaa ggtgtcgtgg
     4681 tagccgaggc cgatgaagct gcgcaccact ttgttccggc tcatcatctg gcgcaaacgg
     4741 cgcagggcgg cctcctcatc cagcggctcg ggcaggttca aaacatcgcg ctggcggatg
     4801 gactcgggca ccacctggtc aatcagtgtg tcgagcgagt ccacgctcag actttgcagc
     4861 atggccgctg tctcttcagc ggtgggaccg atgtgacggg aggaaaaggg gactgacatg
     4921 gaaggccgcc ataggagcta ggaagatgcc gggagacaaa tgcgaaatgc gaaattcgca
     4981 tgttggagtt cagcgttgga gttcaagctt cagcttgttc catgaactcc cgcagcggaa
     5041 tctgctctcg tgtcgcctaa aggcttcact ccaacgttgc gtccaagcat cagcgcagcc
     5101 cggtgtttcg aatctcgaat ttcgagcttc gagccttcag ctcctccatc gccgcgacga
     5161 tcacggaggg gtccatttca tgcacctcca gcttgcggcc cacttcgggc acgagtgtca
     5221 cggtcagctc gccgccgagg tgctcgcgga attcctccag cccgtccagg atcaccgggc
     5281 cgccgcgctg cccctcggcc agcagaccgt ggtggtaggt gtggaagccg aggctttcca
     5341 ccaggcgcag cacgcgctgc gcggtcgctt cgtcgagcag gccggttttc acggaataca
     5401 cgaggtccac cgccatgccg atcgccaccg cctcgccatg cgtgacctcg aagcgggtga
     5461 tctgctccag cttgtgcgcg gcccagtggc cgaagtccag cgggcgcgcg ctgcccagct
     5521 caaaggggtc cccgcccgtg gcgatgtgat ccacatgcaa ctcggcgctg cgctggacga
     5581 cgcgctcgag cgcgggctgc tccagccgcg ccagctccgg cgccaggcgc tcgatctctt
     5641 caaaaaacgc gcggtcgcgg atgagggaga ctttgatggc ctcaatgatg ccgttgcggc
     5701 gctcgcgcgc gggcaggctt tcgagaaaag cgaagtcgtt caccaccgca taaggcaccg
     5761 cgaagctgcc gacccagttc tttttgccga aaaaattcac gccgttcttc acgcccacac
     5821 cgccatcccc ctggctgagc gtggtggtgg ggaagcgcgc atggcggatg ccgcggtggg
     5881 ccgtggaggc tgcaaagcag acgagatcca gcaccgcccc gccgccgatc acgaagacgt
     5941 agctgtgccg gtccagcccg gcctcgtgga tggcctccca gcagtcctcg acgagggcga
     6001 agtcgttttt gcacgattcc ccgcccggca gctccaccac ttcccccgcc agatcataga
     6061 cctccgcatg ggcgcgggcg tgggcgcgga tggactcgcg caggccgggg ttcgccgcca
     6121 gcacttgatc atccgcgaag acgagcacgc gcggcggctt cttcggcgag tcgagcagca
     6181 gcaggtcgcg gatgcagggg ttcgcgggcg cgaaggcttg gcgcgtgaaa aggatgcggt
     6241 ggctgtgctc gagcctgatt ttcttctcca acatgggcgg gggtatcctt tgcccgcaaa
     6301 caaaggcgct ttcaaaggga aaggtgaaac gggcgcggcg gcggattcta acggacgggc
     6361 gtcccgctca tgtcgccgcg atccagcgct gccacagccg cagcagcggg gccagggcgg
     6421 cgaagccgca agcaagcgtt agggacacat gccccaccgc gagcgcgtcc acgatgggaa
     6481 tgccggccaa cagccagccc actgcgcgac caacatgagg accacctttg cgcatgattc
     6541 gcatggagag aaaaacccaa atggcaaaga ctggcagcat cagcagagga agccagtccg
     6601 agggttccgc atccacggcc acccaaaaaa atgtaaatgt gaatgcaacg gccaaaatag
     6661 ccgggaccca aagcaggagc ttggccatga ccagcgatag cacgacggca ccttccttgc
     6721 tggactcaag gcgcgccact ttggtcaaac ctacaatgta aacgccgagc gcgatgccgt
     6781 gcggcgtcgc caacatctca ccagacatct tgcagcccgt gtcgttagca gaagcggcca
     6841 gaagcaccag caacaaacga catgacccca tcacccaaac agctccgctc caaggcttgt
     6901 gaaagagatc atacaagata acagaagcca ccaatccggc tgcaatccag ggacatgccc
     6961 cgccctgtgc gacggccatg cccgctcctg cggcgagcat ccccgccccg accgcccaag
     7021 ccgcctccgc ggagaccctt cctgacggga tggcccgctc cggtcggtgt tcgcggtcga
     7081 acttcacatc agcggcgtca ttgaggatca tgccgccggt gtaaagcagc gatcccgcga
     7141 gcagaagcca cccaagccgc gcgtcccagc cgccacccgc gagcagccag cccgcgctga
     7201 cattggtcca caccgtgggc aggttggaga tgcgggcgag ttcgagccag gcgcggagca
     7261 tgagcatcca ttaccccgcg cccaatgcac tgccaaatcc agattcacac ccatgctgga
     7321 acccgcgcgt ccgtgaagat cgcgaatttt tcctgtccaa aaccgcgccg acggcgactc
     7381 ttgcagggtg atggatgatg ccaccacagc ctttcttgaa ctgctgacca agcacgagcg
     7441 cccgctctcg ctttatgtcc acggcctggt gccgagggac tcggcggcgg aggacatcct
     7501 ccagcagacg aagatgctgc tgtggaagca cttcaaagac ttcgagccgg gcaccaactt
     7561 cctggcctgg gcgcgcaaga cggcctttca ccagatcctc acccaccgcc gccagaagaa
     7621 gcgcgagcac ctgccgctgg acgaggtggc gctggaggct ctgggccacg ccgtgtcaga
     7681 cctcgccgtc gaaggctccg cccggcagga cgcgctgcgc gcctgcatgg cgcggctgcc
     7741 cgtggagcac cggcggctca tttccctgag gtacttcgag gaaatggaga tcggccagat
     7801 tgccgggcgc atccggcgca gcgaaatggc cgtttaccgc gccctgagcc gcatccgcat
     7861 gacactgatg cagtgcattg aaaaacaaat ggaggcgcat tcatgaaagc ggaggaaacc
     7921 tggcgctggc tggagctgtg ggagcgcgcg cgcgatcgcg cgctgaccca tgaggaggaa
     7981 gccctgctca atgaagccgt gacctgcaat cccgaggtgc gcgccctgct cgcccgtgcc
     8041 gcgttgatgg atgcagagac ggcctgcatg gaaatgccgg aaacgatctc cgcgccctcg
     8101 ccgcgcacgg tttctgtctg gcggcgcgct ctgcttcccg ccgccgcagc tctcgtctcc
     8161 gccggactgg tctggctgtg ggaccgcgag ccgccgcccg tggccacact ggtacgcgcc
     8221 gccgcctgca aatggggaaa cagcgccctg cccaccctgg aggggtccgc cctccgcccc
     8281 ggcctgctgg agttgctcga tggcatcgca accctgaaat tcaaaagcgg tgccgaggtg
     8341 actctcgagg cgcctgtcac gctcgaagtc ctctccacca tggagtgccg ggtgaaaaaa
     8401 ggcaccgtca tggccgaagt gccccccagt gccaagggct tcacaattca cacccccgaa
     8461 accaaggtgg tggactacgg cacacgcttc ggcgttagcg ccagtgagga cggcaaatgc
     8521 ctcgtgcatg tcatggaggg cctcgtcgaa gtcgagcgcg ccggagaaaa aggccgcaaa
     8581 gaattgcgtg ccggcgagcg cgcggactac ggcggcttca tcaaacaagc actcaatccc
     8641 gacgcagacc cgttcgagca gaccgagtcc agccgctggc tgccgggccc catcaaccag
     8701 ctcgccgacg gctggcagct tgtcaccact gcctatggcc gcggcaggga ctcctgggtt
     8761 cagtccagca aacaaaaagt cagcggacgc gagtccttcc tgcgggtgaa attcaccacg
     8821 ctcaatgacg gactcaatcg caaggcttac gccgcctttg acctttccgc cttcgctggc
     8881 aagcgcgtgg ccgaggccga gttcgccctc catgtcgagc ccagcgacct cggcttcgcc
     8941 tcgctcgttc ccgacgccgt cttcaccgtc cacgccctcg ccgatgaagc tcaggacgcg
     9001 tgggacgagg cggaattgtc ctgggaaaac gccccggcgc acagcaccgc gccggagcac
     9061 cggaactcac ctgtcgcggc tcagtccgtc ctcatcggcg agttcaccgt gccccaggga
     9121 accacgcgcg gcgctttctc ggtgaagggc gccgcgctcg tccgcagcct caacgccgac
     9181 accaacggca tgctcaccct catcatctgc cgccagaccg acgagaccgc caccagcggc
     9241 ctcgcccacg cctttgccag caaggaaaac acccgcaaca ccccgcccct gctcaaagtg
     9301 cggctggagt gagcggacgc catgcggttc gatctgccgt cactccgccc cccccaagac
     9361 tccgcccttg aatccccgcc ggatgcccgc catatccagc gcctatgcgc attctctcag
     9421 gactccaacc cagcgggcgt ctccacatcg gcaactattt cggcatgatg gaggcggcgc
     9481 taaagctgca gcatgagggg gaggcctttt acttcatcgc cgactaccac tcgctgacca
     9541 gcatccagga cggcaaactg ctgcgctccc atgtgcgcga cctggcgatt gatttcctgg
     9601 cctgcgggct ggatccggcc aaatgcgtgc ttttccgcca gagtgatgtg ccggagcaca
     9661 cggagctggc ctggatcctc agcacggtga ctcccatggg cctgctggag cgctgccata
     9721 gctacaaaga taagatcgcc aacggcatca gcccctcgca cggcctcttc gcctatcccg
     9781 tgctcatggc ggcggacatc ctcatttatg acagcgatgt ggtgcccgtg ggcagggacc
     9841 agaagcagca cgtggaagtg acgcgggaca tcgccgtgaa gatgaacatg gcctttggcg
     9901 agggcctgct gaaggtgccc gagccgcgca tccgcgagga gaccgccgtg gtgcccggcc
     9961 tggacgggcg caagatgagc aagagctaca acaacaccat ccagctcttc gaggagcccg
    10021 ccgcgctgaa aaagaagatc atgcgcatcg tcatggactc cacgccgctg gaggcgccga
    10081 agccgatcga aaacaacagc gtgctcgccc tctacaagct cgtcgcctcc gaggccgact
    10141 accagacgat ggtggacgac cacctgcgcg gcggcatcgg ctatggcgag ttcaaaaagc
    10201 gcctgctgga ggccatgctc gcgcacttcg cccccttccg cgagcgccgc gccgggatcg
    10261 tcgccgaccc cgcttatgtg aaccgcgtcc tggccgaagg cgctgaaaaa gcccgcgcca
    10321 tcgcccgcca aaccctcaac cgcgtccgcg ccgccgtggg actgggagat tgatgccatg
    10381 cgccgctatt ctggccgcct ctgccagcga aaaatttcga gctgaggttg gcagaacttt
    10441 gaatttgaaa ccacgaattg acacgaatga gcacgaattc cgaatgttgt ggcaagggat
    10501 gcgtgtcgca actggacgct cttttccaga tgctctgtgg gatttggaag aagcgcaccg
    10561 ctgctggcta cattcgtgtc aattcgtgtg cattcgtggt tccctccttt gacctctgct
    10621 tgcgcgggta aaacgaagaa catcccaatc cccatgcaag acggctacga actcctcgac
    10681 agcggcgaat tccagaagct ggagcgcttt ggctccgtgg tgctctcgcg gccctgcgcg
    10741 caggcggtgt ggcggcagag tctgtcaaaa gcggactggc agcgcgccac ggccgctttt
    10801 ttccgcgacg gcggcaacca gtggcgcggg cgcgaccgcc tgcctgcgac gtgggagatc
    10861 agtgtggatg gcatgcgctt ccagctcagc agcacggatt tcggccacct gggcatcttc
    10921 cccgagcagc gcgaccagtg gcggcgcatc cgcgagctgt gcgcggccta ccccaaagcg
    10981 catcagcgcg cgccgcgcgt gctgaacctc ttcgcctact caggcggcag caccctggcg
    11041 gccgcgcacg ccggggccga ggtctgccat gtggacgcct cgaagggcat ggtggactgg
    11101 gcgcggaaaa acgccgcgct caatggcctg gaggaccgcc ccgtccgctg gattgtggac
    11161 gatgtgacca aatttctcga acgcgagctg cgccgctcac gcagctacga cctcgtcatc
    11221 ctcgacccgc ccagctacgg acgcggcgcg aagggggaag tcttcaagat cgaaaacgac
    11281 ctgccgcccc tgctggcgct catcggccag ttgatgtcct cccagccgct cggcgtgctg
    11341 ctttcctgcc acaccccgga gctcacgcag acctcgctgc accacctgct gctccaggag
    11401 ttcgggtcca aaggcggcgc tctggaatcc ggcgagatgc tcttgcgcgg cggcaaagac
    11461 gtgctgcccg tgcccagcgg cagcttttgc tggtgggtga gagcgtgatt tggagcaaag
    11521 cagtgcggta ttgccaagag tctaaaaagt gtctaattgc agtctaatta gacgcttttt
    11581 agactcatgc gtgttccgca agatccaccc tcgacccaaa tactgctcca acgaacgctc
    11641 accagacccc agcgtttgat ggagttggtc gccatgcgat ctgaggattt gccacagcat
    11701 gaagagtatc tgcactggga caaactgcgg catctgtctc cgcctgcggg actcagccat
    11761 gaggagtggt ggtggctggt aaaaagaggc agacaatcag gccggcaagg catcccactg
    11821 cgggacaaaa aaggcgtttc gttcagcttc tcgctggtgc cggaaatgtt tgaggccctg
    11881 catcacatag accagcgctg cgctggtaac atagcgatgc ccgagcaagt cacggacggc
    11941 agaacccggg accgctacca gatcaactct ctgatggagg aggcggtcac atccagcctt
    12001 cttgagggtg ccgcagtcac acgccagaaa gcaagggaca tgctgcgcag cggacgcaag
    12061 ccaactgaca aaagcgagcg catgattgtg aacaatttca agaccatgca gcatcttctg
    12121 aatatcaaag accgcccgct cacaccggag ctggtgtttg agattcatgc catgatctcg
    12181 gaggacgcgc tcgacaagcc cgatggagca ggccgcctgc gccgcgcgga cgagtacatc
    12241 gaggtttccg acgacctgga caatattttt cacatccccc cggcagcgtc ccaactgccc
    12301 gggcgtctgg aagagatgtg tttctttgcc aacgagaccg gggtcaatgg tttcctgcac
    12361 ccggtcatac gctccatcgc gctgcatttt tggctggctt acgaccaccc ttttgtggat
    12421 ggaaacggcc gcactgcacg cgctctcttt tactggtcca tgctgcgcgg cggctactgg
    12481 ctgttccagt tcatctccat ttcccaggcg ctgcttcgtt cgccgtcgga ttattaccgg
    12541 gcttttctgc acaccaagca cgatgagaat gatttgaatt acttcattct tcatcagctc
    12601 cgctgcattg aaacgtccat ccaagacctc catgaatatc tcgcccgcaa atcggagcag
    12661 agacgtgggc tgcaaatcat cttgcgcggc ttggattggg tcaatcaccg ccagcaggct
    12721 ctcctgagtc acgcgctgga gcatgatgac gcagcctata cttttgagag ccaccagcgc
    12781 agtcaccgca tttcgagaat gactgccatt agtgatttgc gttccctgga ggaaaaagga
    12841 ctgctggttt cccgtctcca gggcaggcag cgcgtcttcc gccccattcc aaatctggaa
    12901 acaaaactcg gaacgcgcac tcgtccatga gccgccacct tttggacaca ggcgcggcgg
    12961 caaagacgtg ctgcccgtgc ccagcggcag cttttgctgg tgggtgaagg cgcgggggtg
    13021 aggctgcgcg gggtcaagaa ccgcccactc aacgacacgc gctgcaaagt ccatcgacga
    13081 cttttgttgt atgtttcatg catgttggaa tccaagcccg acatccaagg ccgcgccgag
    13141 atcgagacgc tcgtgaatac tttctacgag cgtgtgcggg ccgacgattt gctcggcttc
    13201 atcttcgaca aggtggcgcg gacgaactgg gagtctcatc tgcccaaaat gtgcgctttt
    13261 tgggaaaccg tgctcttccg cagcggcggc tacacgggca atccgctggc cgctcatgcg
    13321 aaactggtgc cgcgggcggc gatgggccgg gagcagtttg accgctggct gatgctgttt
    13381 cgctccacgg tggatgattt gttcgccggc gagaacgccg ggcacatcaa gaactgcgcc
    13441 gctgacatgg cgaatgtcat ttacgcgcgc atcaaccagg tcgccgatcc gcgcttcgat
    13501 cccgccaacc ttacggcgga gcaaaaggag cgttatgcgc gttacaaagc cagcgcctcc
    13561 acatgagcca gttccccgcc tgcaaacaca agacgcacaa ggccaaaggc ccgcgcaaag
    13621 acggcctgcg ctggctcgcg gctgagccct accgggtgtt cttcgccagc ggcgcggtgt
    13681 ggagcatcat cggcgtctta ctgtggccgc tgtattatgc gcaaatgctg ggcttctatc
    13741 cgaacctcgt tcacgcgcgc ctcatgatcg aggcgtttgg cggggcgttt gtcgttggct
    13801 tcctcggcac ggcggggccg cgcatggcga cggcgccgaa gctcacgccg gcggagttgt
    13861 actggctctt cgcgctgcat caggcctgtg gtgtgagcca tctcacgctg catttcgcat
    13921 ggggggatgg cttcttcatc gcgttgctcg tgtcattggt gctgtgtctc gtgatccgtg
    13981 tggcgaggtt ccgcaaagaa atgccgccgc cgcagatgct gctggcgctc accgggcttg
    14041 tctgcggcat cgcgggcgcg gtgatgcatc tctccccagc cacgctgatg gacccgcagc
    14101 gcctgcgtct ggcgaatctg ctgctctacc agggcctgct gctgccgccg gtgcttggca
    14161 tcgggtcgtt tgtttttccg cgcatgttgg gcggggattt tggcgatccc aaaacggcgg
    14221 cgcaaagcaa ggcgaagctg ctgcgggcca tcgccgtggc ggctttgctc gtcggcagct
    14281 tttttctcga agcacatggc ggggtcgcca ctggccattc cgtgcgcgcc atcgccgctg
    14341 cgggctatct gctcatcgag gtgacatggc agacgcggca aagcggctcg ctcaccaccg
    14401 gtctgttttg ggccctggca accggctggc ttggcatcgt tctggcgcca ttttactacc
    14461 tgcagcatgt ctccatcgag catctgctct acatcggcgg attcggcatg ttgatgctca
    14521 ttgtcggctc gcgcgtgctc ttcggccata gtggtgactt ggaaggcttc ttcgcaaaat
    14581 ccaaatgggc gcgctttttg atcttcctcg gcattctggc cgccacgacg cgcgctaccc
    14641 ccgcctgggt gccctcgacc acgatttccc atcacatcta cgccgcctgg acctggggcc
    14701 tgctggcgct gctctggctg ctgtggcatc ggagacgctt tgtgcggcgt gacggggaac
    14761 cgtgagcggc ggcatcagcg cctgctccgg cgcgcgccgc ttgcactagc cctggcgcgc
    14821 ggctctttgc acgccgtccc gccatgcccg tccatccctc agcttaccag gctccgtttt
    14881 tcctgcgcag ggggcatgcg cagacgatcc tcggcgcact gacgcctgcc tggacccggc
    14941 ccgtgttctc gccggagact ctgcctctgc cggatggcga ctgcctccac ctcggctggc
    15001 tgcggggcgg gcacgcgcgg ctcgtcatcc tcagccacgg gctggaggga tccatgcgcg
    15061 ccgcctatgt ccgcggcatg gccgccgcct gcgcgcgcgc cgggtgggac gtgctggcct
    15121 gggactaccg cggctgcggc gggcgcgcga acctcctgcc ccgctcctac cacagcggcg
    15181 agagcggcga cctgcgccac ctcatcaccc acgccgcgcc cggctatgac agcgtggccc
    15241 tcgtcggttt cagtctcggg gggaacatca ctctcaaata cctcggtgag tccccgccac
    15301 accccgccgt gcgcgccgcc gccgccgtct ccaccccggt ggacctggcc tccagcgccc
    15361 gcgcgcttga tgagcacccc gcctgccggc tctaccagcg ccgctttttg cgctccctgc
    15421 tggcgaaggc ggaggaaaag gcgcgccgct ttccgcgggt gctgccgcct ggtggactcg
    15481 ccggcatccg caccatccgc gacttcgacg agcgtttcac cgcgcccctc cacggcttcc
    15541 gggatgccgc cgattactat gcccgcgcca gttccctgcc ccacctgaac tccatcaccg
    15601 tgcccgccct cctgctcaac gcagcggacg atcccctcct cgagccgccc tcctaccccg
    15661 gcggcgcggc tgctgaaaat gccgccctgc atctggaaat acccgcccac ggcggccatg
    15721 tcggttttct cactcacggc ctgcgccgct ggcacgagcg gcgggtgctc gacttcctgg
    15781 ccaactccag cccctcaaag cccactcact caaggcttca cgcatgaaat ccaggaaaat
    15841 caaagtgccg atggccggac tcgaaccggc aagggagttg cctcccagcg gattttaagt
    15901 ccgctgtgtt taccatttca ccacatcggc agacagaacg gccagtctag cggcatttcc
    15961 cggcaggcca agcggttcct gttttcacac ggttgagcgg atgaaaaaac gtggaaacga
    16021 ccggggggca aaacggggta aaaatttggt tgccggcagg gcgacttgtg gcagcttggc
    16081 ggccctttac gccggaaagc ggttccgcaa gccgcttttt ggcccccggg ctacccttca
    16141 acgcagacaa catcatggca tacgcaatct tcaaaacagg cggcaaacaa tacaaggctt
    16201 ccgtgggcga ccagctcgac gtggagaaga tcgaggttgc cgaaggtgag gccgccaatt
    16261 ttgaccaggt gctggccgcc ggtgaaggcg ccgacatcaa ggtcggagcg cccgtgctcg
    16321 aaggagccag cgtcggcttc aaagtgctca agcagttcaa agcgccgaag gtcaccgcct
    16381 tcaaattccg caagcgcaag ggctaccaca agaccaaggg gcaccgccag ttcctgaccc
    16441 gcgtcgaggt cacctccatc aacgcctgat ttcttttacc caacccctca tcgaactcac
    16501 gacatggctc acaaaaaagg tcaaggcagc gtcaaaaacg gacgcgacag caattccaag
    16561 cgtctcggcg tcaaaaaata cggcggcgag tccgtcatcc ccggaaacat cctcatccgc
    16621 cagcgcggca ccaaatggac tgcgggaagc aatgtcggca tgggccgtga tcacacgctc
    16681 ttcgccctcg tggaaggcaa agtccgcttc gacaaggacg gacgacgcat caacgtggac
    16741 gaggctgttc ttcaggcttg atcccggcca aattttcccg gcggcttccc caaggaagcc
    16801 gcctttttca tgccatgaac cggaccggac tcgcccgcgc gctttcaaag ctgggctttt
    16861 gctcccggtc ccaaggcttc gaactcatcc gcgcaggcca ggtgcggctg aacggtcgga
    16921 tttgcatgaa tcccgaacag cccgtgcttt tggataaaga cgatgtgcgg gtggatgggg
    16981 cctcccttgc cgccgccgcg ccggtttatc tgatgctgaa caaaccgcgc ggcctggtga
    17041 ccacggcgcg tgacgaacag ggccgggaca ccattttcag cctgctcaaa ggcgcgggtc
    17101 tgccgcatct cggacccgtg ggccgactcg acaaggccag cgagggcctg ctcctgctga
    17161 ccaacgacac ctcctgggcg catcgtgtca ccgaccccgc cagccatctt cccaagacct
    17221 accatgtgca aaccgccgga ctcctcggtg aagctgccct cacgcgcttc ggcgccggta
    17281 ttcgtgacga tggagagcgc ctccgcctgc ccgatgcgcg cgtcttgcgg cagggcgaaa
    17341 agaacacctg gctggagatc gtgctcaccg aaggccggaa ccgccacatc cggcgcgtgc
    17401 tcgaagcctt cggcatcgaa gtcctgcgcc tcgtccgtgt cgcagtcggt cccctcgtcc
    17461 tgggagaact tgccaagggc gggtggcgcc atttgaccac gcaggaagtg agcattctcg
    17521 aaaattgacc ggctgcctcc ttcaatgttg cgcaaaccgc cgcgattcct ggccggttca
    17581 tgccattccg cgcgcttgtc gaaaagctgg aaggcactgc gcgtttgtga taaggagcat
    17641 ttatgaaatc cctttcccga ttcactctcg ctgccgcctt cctgctggct ggtaccggcc
    17701 acgcggcgga cgccagggcg gacttcgcca cccagatcaa gcccgttctc ggcacccgct
    17761 gcatcaactg ccaccactcc ggcgcgctct ttggcgagct gaatctggag aaccgcgaga
    17821 tggccttcaa aaagcgcgcc aacggaccgg tcatcatccc cggcaagccg gacagcagca
    17881 tgctttatct cgttctcaag ctgccgccga aagagttgaa ggccatgccg cccaacggcc
    17941 accgtattcc cgaccgggag gtgaatttga tccatgactg gatcaggcag ggcgccgact
    18001 ggcccgccgg tgccgagggt gtgatccagc ctcccaggat gaaaaccgcc agcggctcct
    18061 gagcgttccc agcccgcgtc cgtcacccat gaccgcctgg atcacgcgcc tcgtcctgct
    18121 ctccgggctg attgcggcgc tcgtcttctt tcaccattcc cgcgcgcacc ggccgccgcg
    18181 cgtggatgcg gcgcgggaga agggcatcct gctcgtcggc aacggctcgg accccgccac
    18241 gctcgatccc cacctcgcca caggcacgcc ggagcatgcc attctcgact ccatcttcga
    18301 ggggcttgtc gccgccacgc cggaggatcc ggacgccaac ggtcacggag ccgccgagcg
    18361 ttgggagacg gaggactttg tccactggac ctttcatctg cgcaaaaacg cgcgctggag
    18421 cgatggcgcc cctgtcaccg cccgggactt tttattcgcc ttcgagcgca tcctcacacc
    18481 ggcgctcggc gccgattacg cgcccatgct ttacccgttg cggaacgcgg aggagttcaa
    18541 cacggggaag atccccggct tccaggaagt gggcgccagc gcgccggacg atcacacttt
    18601 gaagctcgtg ctcaaaggcc ccgcgcccta cctgctctcc atgctcaagc attacgcctg
    18661 gtatcccgtg ccgcgccacg tcattgaaag gcacggcaaa atgaccgacc gcgtctcgcc
    18721 atggacccgc ccgggcaacc tcgtcggcaa cggcgcgttt cagctccggg aatggcgcct
    18781 cacgcattac gtctccgtgg agcgcaatcc gcattactgg gacgccgggt cagtgcggct
    18841 caatggcatc cactttttcc ccatcgtcag cgactccacc gaggagcgcg ccttccgcga
    18901 cgggcagctt cacaccacgc tcactttgcc gctggcgaag ataccgctct accagcggca
    18961 gcacccggat ttgctgcgcg ttgacccgat tctctccgtc tatttttacc gctgcaatgt
    19021 cacacgcaag ccgctcgacg acgcgcgggt gcgccgcgcc ctggcgcagg ccgtggaccg
    19081 ctccgccatc gtggaaaaca tcctgcgcgc cggccagaag cccgcgcacg gtctcactcc
    19141 gcccggcagt tcgccggatt acaccacgcc cgatgtgctg cgtttcgacc cggaaaaagc
    19201 ccgcgccctg ctcgccgagg ccggttaccc ggacgggcgc ggctttccac ggttcgaaat
    19261 tctgatcaac accaacgagg cgcaccgctc catagccgag gccgtgcagg agatgtggaa
    19321 aaagcacctc aagatcagca tcggcgtgcg caacgaggac tggggcgtct atctcgacag
    19381 ccagcgcaag ctcgactaca gtgtctgccg cgccgcctgg cagggcgact acctggaccc
    19441 cttcaccttc ctgagcatgt ggaagactgg cgacggcaac aacaacaccg gctggggcag
    19501 cccggcttac gacgcgctca tggacgccag ctcccgcgag cccgacacca cccggcgcat
    19561 ggcccagctc caggaggccg agcgcctcct gctcgacgag ctgcccatgc tgccgcttta
    19621 ctggtacgtc cacacctggc tcaagcgccc cgaagtgcaa ggctggcccc cttccctgct
    19681 cgaccaccgc tgctacaagg ccatccattt tcaggagtcc ggccggcggt aggttatttt
    19741 gacaggcagg cggcttccgc ggggaagttt cccctgcctc tccggcggag attttgtgct
    19801 caggactgcg cccggctcag atggcttcag gattccatat tcgcggcatt cccggcactc
    19861 aaaacgcgcc tgcggagccg cgagcggcct ggattgtctc cgaaagtttt cgcgacaaaa
    19921 ccgcttcagc ggctatcttt tccgcccctt ctcatggcag acttcttcac aaaccccctc
    19981 tacaccttcg gcatgggctt cgccctcggt tgtgtcttcc tcgtcatggc ggtgttcagc
    20041 cattggaaga cgaaaggcga attccgccgc tacaaatccc atttgagcga caagctggaa
    20101 ctcgacgccc gccagctcca ggaaaccaac aaggagcgcg cccggctggc ccaggaaaac
    20161 gagaacctgc gcatccaggt gaaccggctg aacgaaaaac cggacaacaa gctccagcgc
    20221 gagctggaga tcatggcccg cgcggaaaag cacatgctgg tcaacgcgcc cggtttcgcc
    20281 cccgcatggg aaatggccaa gtcacacgcc atgtcgcagc tcgagaccga ggaaaaaggc
    20341 ctgtccttcc cccaaaagat cttccgcaaa ctcatcggcc ctggcgcccc cagcaacaac
    20401 tccggccccg cgcacgaacc cgcctcccat cacgcggaga ccgccaaatc cgacgcggca
    20461 tgactccgcg ccagcggcct cgtcatcaga gcgaatgacg gctctcccgc gggcggcttg
    20521 ccgctggttc gtcgaatcta aacccgggac ttaaatcaac tccacggatt caaaaacttg
    20581 gaggcaacac ccggaagtgc gggcatgagg ttttcaaaat gcgtgatcag gatggtccag
    20641 actccgtgtc caaccgtctg atccgtggtt tccaaccgcg ggttcacgtt gaatccaggc
    20701 cacgcatggt tccgcatttc caggatcaac gtctgcgacg gaaatggaga cccatgagac
    20761 cgaggaacag cagcagcacg cgccccggct ccggcaccat caccatgatc gtgccctgcg
    20821 tgtaaaggtt ggagacatcc caggccaggc cgatcgagct gagatcgggc aggttcatgt
    20881 aattgaaggg ggctggatcg gaaaggttgc tgaacgcgcc cgtcactccg cccgtgagac
    20941 ccgtccagtc aaacagcctc cactccgttc ccaccaccca actttcatct atcggcaggc
    21001 tggtggttac atgcagctgc gcgttggctc cgatggtgaa gccactggaa ccattgacca
    21061 cgagcaagtc attatgcgtc gcgtcattga gccctccgct cccctggccg ctgaacaggt
    21121 cgaatgccac aatgtcattc accgtcacca gcccggcgcc tgtcgtcgtg attgtgagct
    21181 gccgcgcgct cacatccccc gcagtgccga tctgcagtgt gccgtccacc atgacactgt
    21241 tgtcagccgc cggggcgagg aagccgttgc cgcccagcgt ggctcccggg gccaccacga
    21301 ccgcgccagt gccggtggcg gaaccggtcg tgtttgtggc gagcagggtt cccgcgccca
    21361 ccgtggtgat gcccgtgtag gtgtttgcgt tgctcaagac aacagtgccc gcgccaacct
    21421 tctccaaacc acccgccacc gcgccgccga tgacaccgct gagggtcagc gtgccgcccg
    21481 tgtccgctcc cacatcgaag tttcctgctg tcgtttctgc gatggtgaca acgcctgaat
    21541 acacacccgc ggacgctccg ccatggaggc ggagggtctt gttgttgccc gtgttgctga
    21601 cggtcaggtc gttggctatc gcgatcccgt ccgcgatttc gagcacggaa tccgtgttga
    21661 ccagcgtcac gaccccggcg ccaaacgcgc tgttgctggc cgcccgcagc acgccacccc
    21721 cggcggtggt gtttccagtg tgggtgttgg ctccattgag cacccaggtg tttgcatccg
    21781 acttggtcac cgagagaatt ccaagcgtgc cgccgtccag cgcgcctatg gagttttcaa
    21841 cggaaggatc cgatgtgccg cgcagggtca gcgtcttgtt gccgccgcgc atcatgaccg
    21901 cgcctccaag ttcgaacgcg cccgtgccat ccgcggacaa agtcacccct ccggttgtcc
    21961 cctggagtcc gaccagacgg tcgctggcat gtcccgcgcc tgtgtaagcc agtgtcgtgg
    22021 ccccggtggt cagccccatt cggatgatgc cctgccgggc gttcaaagga ttgcccaggg
    22081 cgctggatcc gcccgcgaca ttgcccatgc tgtcaatgct cagagtcccc gcctggattt
    22141 ccgtgatgcc ggtgtaagtg ctcgcgccgg agagtgccag cacattgttt tgggtttttg
    22201 tcaggcccac aggtccagcc agcgcggctc cgacaagggt gtctcccgtg tggccaaagg
    22261 caaagccctg ggtggagtaa atggtgcccg tgccattaat caccgggctt tcaccacttg
    22321 tggtgacacg gccgacagtg acctcgctcg cgccaacatc gagaactgcc ccgcccaaaa
    22381 tcgcgaggtt gccactcccg atggagttgc tgttcccaag tgtgagagtg ccgccggcaa
    22441 cagtcgtcgg acctgtgaga gtcgcgtcgg cgttgagggt gaggttgcca agacctgtct
    22501 tcgtaaaccc acccggcccg gaaccgagcg tcccggcaag ggccaagtcg gatccattcg
    22561 tgtccatgac tccacctccc tcaaggaaga gaatcgagcg ctgggagatg tcatccgtga
    22621 agccggcccc cagccggagg acgccgcccg caaaaaccag cccgccgctg ttgcctccaa
    22681 tgttcgcgag tccggtgatt tcgagcaccc cctcattgag tgtcgtcttg ctggcgccac
    22741 cacccgccgt attggccgcg gaaagcacca gggtgccgcg tccggatttg gtgatgtccg
    22801 ctgttgatgt cagcggcgac gccagcgtcg ccaccagaga tgcggcgttt gcggcggctg
    22861 agggattgat cacatgtatc acatactcgc tcccgccaac agtgatcccg tcatcaaacc
    22921 cgccgaacag agtattgtag gccgtggagt cggctccgcc tgtgagagtg aacaaaaagg
    22981 caccgctggt attcgccagc acctgaccgg ctcccgatcc tgtgaactcc aagccggtga
    23041 ccgcctcatt gttgacaacc agcgagttca gcacgcgccc gccaacgccc gtgagattgg
    23101 cggacaagga ctcgcgggtg ttgttggtgt ctcccgccgt gctgaaagcc gcgtactccg
    23161 ttgaaaagtc gagcggacgg aagcccgagc ctgcggcata agtgagcagg ctgttgccca
    23221 tgttggtgtt gccgagcgcg ccggcgagcg attcgccaat cgcccatggc acaatgtcga
    23281 gcgtggcacc tccagcggtt ccacccccgc ccgccaagtt ggccggattc gccatgaaga
    23341 cggcctcgct ggcgcccgtg atctcgaact gcgagcgctg cacattggcg gtggccagac
    23401 cgaggacggt gccgcggacg ttgatcgtgg cgttgttgtt gcgcaccagg tcgctcatgc
    23461 gcagatcggg gtcgtcattg gccgtggtgg cctcgatcgt cacataactt gcgccggagt
    23521 tggctgtcac gacacccacg acctcggtgt tggtgctctc ctggtccgtg cgcatgccaa
    23581 gaccgcgcgg cagattgatt gtggctccgc tgaacccgcc gtcggcggag ttgaggatga
    23641 tttcggaggt gtccagtatc ctggtggcaa tgcgggtggt gccattgttg tccatcagca
    23701 ggttgccgcc tttgttgatg gtgaaggtgg tggcgttgtt ctggccgata ttgctcagga
    23761 tgaatgttcc gccattgatc accacctccc cggtgaagcc gctgctgttg ttctgcaggt
    23821 tcagatttcc cgtgctgccc gtgttcatga cgatcgaacc cgagccggtg aacacacccg
    23881 cgtaagtggt gttggcgctc tggttcaatg tcagtgtgtg gccgccgaca gccaccgtgc
    23941 cgtattccga attggtggcg cgtttgccgc cggaaagcct gccgatggtt tcatccgccg
    24001 cgagttgcag aatgctgtgg cggttcagtg cgagagtgac aatcgaagtg tcgccgatcg
    24061 cgttgccgcc gcccgcgaca agagtgccct gcaaaatctc ggtctcatcc gtgtaggtgt
    24121 tgtatcccga aagcaggacg acgccctcgc cggatttggt gaaggcgtgg ttggtgatga
    24181 tggaggagga gatttcaaac acccgcccgc tgtcctgtgt gacgatgatc tcggaagtgc
    24241 cggaagtgag attgccgccg aagaccgagg attcgccagc atccacactg gacgtcacca
    24301 gcaaccctcc cgaggcgatg tgcagcgcgc caagcgggct gacgcccaga ttcgatccgc
    24361 ccgaggcgtc aaaacgcagg ctgttgatgg cggcccgcga cagggtccct gaaaacccgg
    24421 tcgtctcatc cgtgatgtga tcacccgccg cccagatcga aaggtcattt ttggcgctgg
    24481 aaacgagggc cacgatgttg ttgccgcttt tggtggcgaa ccaggtgccc gtgccgtcct
    24541 cgaccgtggc aaaaccggag gatcccagaa ggccgttggt caggcccgtg gtagtcgtga
    24601 cgccgtgtgt gtcgctttgg actcctgagg gcagcacaaa gcgcacggtg ccgtcccggt
    24661 tggcattcgt ccgggtgatg gcccccaggt tcaggacgat ctcctggccg ctgcccggtg
    24721 agagcgtgac gcgattggcg ccgccgttgt ccgctgtggc gctgccgtcc aggctgaaac
    24781 tcgcgacagt ctggctggtg gatgccgagc cgttgccggt caatgtcaga tcacctccaa
    24841 tcatttcgag ctgcgagcct tcgcgcagtt tggtcgtgtt gctgaggctg tagtcgagaa
    24901 cgagcagacc ctcgtttaga atcgtgcgcc ccgtggcggc cagtccgctg ttgtcgcccc
    24961 tgaaggtcac agttccaggc ccgaacttct ccagagtggc cgtgcccgtg ctggcgaagt
    25021 tggcgttaat ctctcccgca tagaggtcaa tgtcgcccag caagaccgtc agggtgccag
    25081 tgccgttgac aataccgctc cggtcggcgg cgcgctcgcc aaggataaat ctgccggatt
    25141 cgagatcgaa ggcgcccagg ttcagaatac ccggctcccc gcctgccgac tgcccgatga
    25201 agagacggta ggggatggtc atgtccacag cattgtcggc tccgatgttc accgtggagc
    25261 cgttgttgat gttgagatca cctgacgccg ctccggagag gaagcgcaac acacccgtgc
    25321 tgccgagatt caagatcgtc gaggttccct ggacggtcat attgtcggcg acagtgtttt
    25381 gcattcccga caacgtccat gtcccgccgc tgaccagggc gtcggccgct gtgcccgtca
    25441 tggtgaaccc tcccgtgatc gcgcccgcgc tgccggcggt gccggcgaga atcaggcggt
    25501 tgccggacgt gtctatgccc actgtgatgg cgccagcgta tgtcattgaa gcgccgttgg
    25561 tgccattgag tgaaagagtg gagttcgcgg tgctggtgaa gaccggacgg tctgtggtct
    25621 gactcaccga gtctccatcg aagcgcaggg tgccgcccgt catgtggatg gcgccgccct
    25681 ggccaaggct gctggccgga ccgccgatgt tcgacacggt ggtgaatgcc agcgttcccg
    25741 cagtgatggt cacatccccc gcgaaggagt tcgcgtcatt attcaggatc atcaccccgc
    25801 tggcgctctt ggtcagcgtg ccggtgccgc tgatcaaatt ggacactgtg aaagtgccgg
    25861 tgcggttgta aaccagcgcg ccgttgttca ccacattgcc ggcgccgatg gagcccgctc
    25921 ccgcgccggc tccgatcgtg agcgttcccg cgctgatggt cgttgtcccg agataaagat
    25981 cattgttcgc cgaaagggtg agcactccat cgcccgcctt ggttagattg aaagcgccgg
    26041 acaatgctcc gctcaccagc agggaggaac cattggcccc ggtgcccgcc acagtccagg
    26101 tctgttccgc gccgagcagg acaggcgcgc tgatggcgac cgccccggca ttgtccccga
    26161 catcaatgcc atccgtgctg gcggaaggcg cgagggtgag gccgaaggcc gaactaaacc
    26221 ccggggcgat ggataccgag gtcacaccgg cgggtgcgct ggcaaacttc aaatcattga
    26281 tgaagtagtg attgtccaga gtggtggcga gggacggacc tgaggccgcc gttgaggaga
    26341 aaatgaccgt gttggccgag ccgggcgagc ctccagaatc aagcgtgccc gccgcatccg
    26401 tggagaagtt cgagtttgaa ccgctcaact gcgcccaact gccgctcaga tcaccctgcc
    26461 agtaaagatc acccgcagcg atggccgcaa gtccgagctg gaccagcgtg tcagaggttg
    26521 tgagagagta agtgaagccg ccaggcatgt ggttcagctc gtatgccgcc ccgctcaagc
    26581 cgctgcccgc ggagatcagg tcataactcg cggcggaacc aaaaccggcg agacccacga
    26641 tgttgatctg aatggtgttc gctgtggtcg ccgccccgac ggccgccagc cggtcagagg
    26701 ccgaagtgat ggcgcccagc tccatgccca ggatcgtcat tcccgccccg gcgcccaggc
    26761 tcagggtgtt gagaccggtg aggttttccg ccgtgctgtt ataaaggttg agcgtcgcgc
    26821 ctccggccac cgtgatgcct gtcgtggcgg tgaggggagc cgacgcattt aaagtgccgg
    26881 cttcgatggc ggtcgtgccg gagtagtcca gcgttgtcgt tccagagccg ccgccgaggt
    26941 tggcaacacc ggcacccgtc ttaacaagcg agagtttgcc cgagccggtg tcggtgatcg
    27001 aatatatgcc cgcccctgtg ccgaagttca ccgcggcatc gttggcccca aacgtcagtg
    27061 tcgcgtcacc ggacgcggtg ttgtctatga cagcggtgcc atcggtggtc gaagtcaggc
    27121 cgttcactgt ttcattgaaa ccattgaggt cgagagtggc ggtgtcgcca ttggccagct
    27181 cggggctgat gatcagattt ccaaaacccg cgccgtgcgg gatgatctca ttggcaccgg
    27241 agcgcactgt gccccggtca atgtcgaggt tcgtgttgtt ctggttgaag ttgctgcctg
    27301 cgccgttgag ctgaagaacg ccgttgccgg tcttgacgat ggaaagccca aacgcggtgg
    27361 aggtggtgat gtcgccgttg atgacggtct ccccgttgcc ctcgatggtc caggtgcggt
    27421 tggcggtgat gctgtcggct gtcacccccc cgaaagtgag gacgcctccc ggcagaatgt
    27481 tgttggtggt caccgagggg ttgctcgtcg tggacagcag ggcgtaggcg tttgtgaaag
    27541 tgaggctgtg gtcgcctatg aacgcaccgg tcgtgttgtt ggtctgctcg acggcattgg
    27601 tgatcgtcat gtcgccgccg aaggcgaaaa cgctgccatt ggcaagacgc agcgcgcctg
    27661 acccgatggc gcccgggtct gcgatgccaa gcgctcctcc gcccgctgtg gtcagaccgg
    27721 tgtagttgtt cgccgggttg gtcagaatcc aggtcgcgcc gccgtccacc gtcacgccca
    27781 gtgtgccgcc attgtccgaa agctggctgg tgatcttgtt gccctcggcg ttggtgccgc
    27841 gcagaaagag agtcttgttg cccgtggccg tgatcacgtc gttggccaca ttggtcaaaa
    27901 tcagggcgcc cgagccatcc gcgtggatct gcgtgctgcc ggtggtggtg tcgaaccgga
    27961 ttttcctgtc gctggtttca cccgccccga ggtactgcag gatgcccgca ccagtgccac
    28021 cgttgccgag agtgatggcg ttggtgttgt cgaaggcgac tccgctgacg ccgacactgg
    28081 tgggagtgct tgccgcgtct ccgctatggc cgaggctgag cacggccagg gtgccctggt
    28141 tcacattggt ctcgccgctg taaacattgc cgccggtcag gcgcaacacg ccgacaccga
    28201 ctttgcgaat gcctgtgacc gcatctccgg cggaaatcgc cccgctgatg gtggcaaaat
    28261 cggcccctgt gttgccattg tcatccacgc ggatggctcg cagatgcccg tcattgattt
    28321 cgccattggt atagttgccc gcgatgccgc tcgaattggc agtgttcgcg ctcatctgaa
    28381 actgtgtggc gctaagaata ctgacgatgt aggcgccgga cggcacattg gtcccggtga
    28441 agctctgtcc gaccgccagt ccggcagtgg aggcgactgt gaccgtcgcg ctgttttgcg
    28501 ccaatgtata gctgtgccca gggccgaatg tcgcccctgc cgcagtgccc agggaaaagt
    28561 caccggcgat ctccacctct gaaagcgcga tggaacccgt cgccccctga gcgcgcgcct
    28621 gggagccgtt cagaatgaga ccgttccggg tgtctaggaa cccggcagtg acaccccact
    28681 cgggtgtgcc cgcccagttg acagtcagct tttcgtctcc ccccaggaaa ccacccgcaa
    28741 actgcgctgt gttggccgca ggcgcggcga accgcatttc tcccagcccc gtgccgatgt
    28801 tccggttcaa agcgccgctg ctgtagagtg ccccgccgtc gaaaacgagg ttggaaccgg
    28861 atgacaagcc ctgcccgtcc accgccatga gaatgccttg cgtaatcatc gtggcgccgc
    28921 tgtatgtgtt ggcgctgttt ccgagccgcc agatgcccgt cccaactttc gtcacactgg
    28981 tggcgaagcc ggtgctgtca gtgatctgcg ggttgaaggt attgaaaccc tgggaggttc
    29041 ccgtgaatgt gatcgtgcgg tcggcgttcg ttccgtgaac aatggcgccg tcgttgctcc
    29101 agatgatggc gttgttcaga ttggtagtcg gcgtgctgcc cagagtgacc cccgatccgc
    29161 tgaccgtgaa caaacggttc gtgctcgctg aggttgatcc aagattgagg atgccgttaa
    29221 ccaggttgcc acgataggaa agacccccgg tggagccgtc aaagaccagg ttggacgcgt
    29281 cattgctgga ggctccgatg ccgctggcaa ggccgccatc ggccagcaca tccacggtga
    29341 ccaatcccgt gcctccgatg gtcgtggctc cgctgtaggt gctcaaacca agccagcttt
    29401 gcgcgccgtt gcccaccttg gtcacatgaa tggcacccgc tccgtcctgg atgctcccga
    29461 aagaggtgcc ggtgccgtta ttgtttccga gctgaatcgt ggccgaagtg gcgctgctgt
    29521 tggtcacgac tccgttgttg ttgaactggc cgatggaggt ccccgtgccg actccgttca
    29581 aatccagcac ggctccctga cggatgttgg tggtggcgtt cgcgccgctc agcaccccgc
    29641 ttccagccag cctcagcaca ccctcgtcaa tgctggttgt tcccgtgcgc gtgctggcgc
    29701 cggacaggat caa
//
LOCUS       DAOMYJ010000038        29441 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Prosthecobacter sp. isolate AMR_MDS_5629
            NODE_559_length_29441_cov_21.332335, whole genome shotgun sequence.
ACCESSION   DAOMYJ010000038 DAOMYJ010000000
VERSION     DAOMYJ010000038.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867850
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prosthecobacter sp. (wastewater metagenome)
  ORGANISM  Prosthecobacter sp.
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Verrucomicrobiaceae; Prosthecobacter.
REFERENCE   1  (bases 1 to 29441)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 29441)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.30
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:34:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,742
            CDSs (total)                      :: 4,684
            Genes (coding)                    :: 4,676
            CDSs (with protein)               :: 4,676
            Genes (RNA)                       :: 58
            tRNAs                             :: 55
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29441
                     /organism="Prosthecobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_559_length_29441_cov_21.332335"
                     /isolate="AMR_MDS_5629"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1965333"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..387)
                     /locus_tag="PLP58_06085"
     CDS             complement(<1..387)
                     /locus_tag="PLP58_06085"
                     /inference="COORDINATES: protein motif:HMM:NF013216.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HRJ08117.1"
                     /translation="MKPQTGTADPGARHGQPPGIRRMALEVLLDWEKTGRFAAELLDE
                     RARRAVLSGPNAAFLHDIVLTCLRNLSLLDHWASHLTGGKKFDAHTRGSLRSGLCQLL
                     LLGVPAHAAVNETVAAAGRAGALVNAV"
     gene            complement(384..1700)
                     /locus_tag="PLP58_06090"
     CDS             complement(384..1700)
                     /locus_tag="PLP58_06090"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009961486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose/GDP-mannose dehydrogenase family
                     protein"
                     /protein_id="HRJ08118.1"
                     /translation="MKLTIIGSGYVGLTTGACFAEVGHHVVCVDNDPKKIEKLVAGEI
                     PIYEPGLEALVKKNVAARRLRFTTSTEEGVDHGEVLFIAVPTPPQEDGSVDLSFMEKV
                     AREISQYLESYRVVVDKSTVPVRTGERVAQTIRRYAKPGVEFDVVSNPEFLREGSAVE
                     DLMKPDRIVIGGNSDRALALMQKIYEPFVAPVLVTDINSAELIKHAANSFLALKISYA
                     NALAEICEASGADVLKVAEGIGADKRIGRGFLNAGLGYGGSCFPKDIAAFIAISEQLG
                     VPFHLLKEVQKINARQLERFLDAMREALWVLKDKKICVWGLTFKPNTDDVRSSVAVAL
                     VERLVAEGARVTVYDPKGMEKARALHFASAVTFAASPLEAAKGAEALAIATEWAEFAS
                     VDLSELREAMMTPLVFDGRNLLDPAAARDFGFQYRGIGRGVIAERG"
     gene            1832..3580
                     /locus_tag="PLP58_06095"
     CDS             1832..3580
                     /locus_tag="PLP58_06095"
                     /inference="COORDINATES: protein motif:HMM:NF017932.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent peptidase"
                     /protein_id="HRJ08119.1"
                     /translation="MKHICIALLLLARPALSLDRPSLQIINAAPDAVEVFWLAPDGKR
                     VSNGRVGPGEDKIIGTTLGHRFVIVDGRRETEVVSKARVQGFRYDPAARDGIPAFYTQ
                     VVHAGGYPICASENVSPYALKEAAYLVDLMLAKRPDVREAMIASGSRLCIIAHNEFTT
                     GLPEFGRLGPVKGYEELAPQDYWDARARGTGGSLTDPFCTCGEENLLAYEGDPYDAEN
                     ILIHEIAHNIHLRGMTNVDPAFDGRLKSAYDEAMKAGLWKGLYASRNHHEYFAEGVQS
                     WFDDNRENDHDHNHVNTRAELTAYDPGLAAMCREVFGDTVLKYTKPRTRLVAHLAGYD
                     PAKAPKFEWPERLIKARALIRKKAVEAHAAASLEEKTHATRAIAGWTVNMSLQLLRDD
                     AVALEKALTLLRAQLDEIARVVPPGAVAELRKVPLWINPEYPGARPRAEYHPGAGWLR
                     ENGRDPVMEKAVEFTNVRVFEQETRRMPNFALHELAHAFHDRVLGFDNAEIKAAYEKA
                     AAAGGYEKVRRRDAEGRMRLDKAYAMTNAKEYFAECTEAFFSRNDFFPFTREQLRAHD
                     PEMFALLGKLWGTSEG"
     gene            complement(3589..4350)
                     /locus_tag="PLP58_06100"
     CDS             complement(3589..4350)
                     /locus_tag="PLP58_06100"
                     /inference="COORDINATES: protein motif:HMM:NF020037.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HRJ08120.1"
                     /translation="MKAAAAAFSGILSQKGGLLIDARLVEQIADLMHDTAFFIKDAAG
                     RYLVVNQSLVERHGLAEKAQMVGRRPCDVCPGDYGRIPTEQDEQVLRTGRPIIERLEL
                     FWRRPNVPVWGLTSKIPIRDGTGRVTGLIGISKDLTALVSRDEVQPAVALALRHLERS
                     YDQPVSPSSLAKKAGMSAARFARVVKRIHGLSPMQLITKTRITAGSRLLRETNTSIAE
                     IALECGFADHSAFTRAFRAVTGLSPTEYRRQDRLR"
     gene            4510..7233
                     /locus_tag="PLP58_06105"
     CDS             4510..7233
                     /locus_tag="PLP58_06105"
                     /inference="COORDINATES: protein motif:HMM:NF019259.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1592 domain-containing protein"
                     /protein_id="HRJ08121.1"
                     /translation="MRFPTLVMILLTSSAMGADFRGFLETHCLECHDSEMKKGGLDLS
                     SFIDEAAVMRDRSIWRSVFEKIESHQMPPPKQKSQPTEAQRQELMTWIMDIAARPDAD
                     LGAVDPGKPALRRLTRLEYNNAIRDLFGLEMDLFMFPERLPIRDKGYFQPANGKMPDE
                     LKIEMREYGAKYPVLCPQLGLPGDNRAEHGYRNRGDAQDFSPLLLEKYLAAALEIVNA
                     PELPRRSRVFTELVGMKQEMQEPADVSSNAPTLAPVHSPDIERAAKAEGSPDYLEPFR
                     EHVASAHADGRGGVFDVPRALANQTIAGKGGLIRAALGARALTINPDSDLWLASFATT
                     KPASTPAILTNKVKGVKTFELTFDIVSEDDDEGIERLGVCVMGRQKQSGMVKLTAVFT
                     DGTDAAITAFIAEGAAGTTFYSFAAPSGEAIKKLAVDGSQFSGDYVLLDDLGIITNGA
                     TQTLLSVQGREPAKPEKKEGQTRVSVSLTPHERIEAFMSRAFRREVADEELAPIFSIF
                     QNARKSGRSEADAMRTAIAAVLSSPGFLYVEANGDATAGKVSRLEDHELGTRLALFLW
                     SSTPDDKLLALAKAGKLRDNLENQVRRMLKERRSRELSESFAVQWLRLDQLHTSKPDR
                     DLFPGFYSGPQGKSTLHGATLAEALLLFETVLVEDRGILDFLAADYTWLNAPLARLYG
                     LQFSAEDHAPEVATNTNRELKTKNDKAALWHRVKLSDANRGGFMSMAAPLVVTSLPFR
                     TSPVKRGAWMLETLFNRPPTEPKVAFAIENDTKEAAQQMSIREKFEAHRNKAACYSCH
                     IRLDPPGFALERFNPIGQWDEKADTKGEWSGSAFDGPAGFKSILARHPHEFTRGFIEH
                     LLSYALGRKLEVYDMPVVDHIQRAAAADGWKFSRIIVEIVRSYPFTHVRRS"
     gene            7230..8468
                     /locus_tag="PLP58_06110"
     CDS             7230..8468
                     /locus_tag="PLP58_06110"
                     /inference="COORDINATES: protein motif:HMM:NF019222.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1552 domain-containing protein"
                     /protein_id="HRJ08122.1"
                     /translation="MMNRRHFLRSTGITLGLPLFESLAKAAPAASPRRFAFIYTPNGY
                     LQSKLLPQKAGQGLNPLTLTSTLEPLSKIKDDLTFITGLDRQFVPGTGVHAQAGSCWL
                     TSSAPQETLDGGFPTNLTLDQLIARQIGADTALPSLELSTNDFTDNKETKYFECISWY
                     GPGYAANTEKNPRAVFQRLFGQGQGGPQRSILDAVLAQAHSLEKRLGGEDKQKLGEYL
                     ESVRSTEQRIQRAGAAAQRIGRPALAEPKGIPEKRSEYLKLMAELFIHAFQNDLTRVA
                     TLLVAPERWDNPTLYDGLFDKPQNHHVLTHTKGDEAKDKLALIDRYHMEFYAHVVQRL
                     KATSLLESTTLVMGSGISDGDQHNYADLQVFIAGGGWKRGHFHYEGKRPLADLWLTMA
                     QQAGVKQERFADSTGILTEI"
     gene            8471..10750
                     /locus_tag="PLP58_06115"
     CDS             8471..10750
                     /locus_tag="PLP58_06115"
                     /inference="COORDINATES: protein motif:HMM:NF019259.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1592 domain-containing protein"
                     /protein_id="HRJ08123.1"
                     /translation="MSLTTETPSTQRTTQKNPLCAPCLCGSKAKLTNLFSAFLCALCG
                     FLHAAQPPRAALERFCIDCHDAETKKGGVDLDSILQDGIAKHPATWEKVVKQLNARHM
                     PPIGKKRPDEAGYEALITALTTELDPQPPKAIAGPAFRRLTRTEYQNAVRDLLAVEFD
                     AKAAFPMDEISHGFDNITVGDLPPALLDRYISAAQKIARQAVGTSTQPEITAIRVRPD
                     ITQEYHVPGLPLGTRGGVLVKHVFAQSGDYEVHVRLQRDRNEQVEGMRGQHQLQILLD
                     REVQSEFTVQPAKDKNYEKIDAHLKARFHVAGGQHDLGVTFLPNGSPVLERLRQPYKS
                     SYNLHRHPRLNPAVYQVTITGPLKAEGAGDTPSRRLIFRASKTDPEAILRPILRRAWR
                     RDITATDLERMLPFVREAPSLEKGIESALAAILVSREFLFRTEQNGDEAALASKLAFF
                     LWSSLPDDELLSQRPLDLAAQARRMLRDPRAKALVTNFADQWLYLRNLDAITPDARLF
                     PDFDHNLRESLRTETSLLFADVIENDRSVLDLLRTDQTWLDERLAAHYGIPHIRGSRF
                     REVKLDSPTQRGGLLRQGSILTVTSYATRTSPVIRGHWILKNLLATEPPPPPPNVPAL
                     DGVISESLPIRERLAKHREMTACASCHNLMDPVGFALENYDAIGRWRAEADARGGFAD
                     GSEFEGVSGLEDTLMKRPELFVTALTEKLIVYALGRGLEASDMPAVRKIVSRAKANGW
                     KFSEIIIGIVTSTPFAASK"
     gene            complement(10804..11127)
                     /locus_tag="PLP58_06120"
     CDS             complement(10804..11127)
                     /locus_tag="PLP58_06120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ08124.1"
                     /translation="MNLDHSVYPDLAEPPLELHTMADRADYVHRICGAFDFGVFPERD
                     DWKLFAGWKDVFDWIPLRDSPAYHTFRSRFGWEPVTHGSHGLPFHWKMADEREGRIDP
                     CEDWV"
     gene            complement(11124..11813)
                     /locus_tag="PLP58_06125"
     CDS             complement(11124..11813)
                     /locus_tag="PLP58_06125"
                     /inference="COORDINATES: protein motif:HMM:NF020419.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="HRJ08125.1"
                     /translation="MQNIRPKPPQNLNPFSRELLESLAGHAEAAEIVLGGGVALSHYL
                     EYRETVDLDAWWRESPRPEVLAFVEGAMKSLAARHGLDYRRRTWGETESLELMRGGQK
                     EFSFQISKRGLYLDEPLPAEWQPLWIETLRDNVASKMTALVERGAPRDLRDIHQLCTQ
                     SITTIEECWAMWQAKNTHRDPPEGPGKVLFYVEKLELMRPVSKLPAESRAQAAALRSW
                     YRDVFCPAGPR"
     gene            11899..13227
                     /locus_tag="PLP58_06130"
     CDS             11899..13227
                     /locus_tag="PLP58_06130"
                     /inference="COORDINATES: protein motif:HMM:NF019222.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1552 domain-containing protein"
                     /protein_id="HRJ08126.1"
                     /translation="MSTVTTRKALSRRHLLRGVGATLSLPFLEAMIPAATAANRVAQP
                     AKRLSYIYIPMGTDHSRWNLGATSTLEQLSPILKSLEPVRQHCAVVSNLELEPAYPGT
                     HATSNASFLSAARVKHTESSDYYNGTTADQIAAKAIGQQTQLPSLELAMDLMQVVGQC
                     DNGYACVYQNNLSWSSPTTPLPAEAHPRLVFEQLFGDGGSKTDRQAALERRASLLDFI
                     KDDMQRLAKQLGPQDRDRVNQYLDSIREVERRIEKAQAAVAENELPDLSRPSGVPASY
                     AEHARLMFDLQLLAMQGDVTRVITFQLARETSNRTYPEIGVSDPHHPLSHHGNDPAKI
                     ERMSKINAFHVSLFAEYLAKLAAVKEGDGTLLDHTLLLYGSGMGNPNVHDHTNLPIIV
                     AGGASAGVKGNRHLRNNQTIPLANLHLTLLDKAGVKLDKFGDSSGIIEVL"
     gene            13237..13881
                     /locus_tag="PLP58_06135"
     CDS             13237..13881
                     /locus_tag="PLP58_06135"
                     /inference="COORDINATES: protein motif:HMM:NF022399.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2625 family protein"
                     /protein_id="HRJ08127.1"
                     /translation="MRPLSELINPDDSALPIIEELAAKVSGRCVVLPPRPDSGEILYR
                     TQVTTRSPMGAVVYHTGGIVVDRWIRIIGSGSSIIERNLPDWNSSRANGFYLIGDDVV
                     GGFFAIDGGGLGFEKGHVCYWAPDSLEWISMERGYTDFLVWSMTMDTTDFYGDLRWSS
                     WEAEVTSVPGDRCFGFYPFLWTVEGSASSSKRAIVTVEEAFDMKMDICRQLSEP"
     gene            13878..15374
                     /locus_tag="PLP58_06140"
     CDS             13878..15374
                     /locus_tag="PLP58_06140"
                     /inference="COORDINATES: protein motif:HMM:NF024206.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HRJ08128.1"
                     /translation="MSLPLLPIELALLTLFFGNLVATDLPTAAEKQDWNSVSAQIASK
                     SDLNATQADGATALHWAAYHDKAEIVEKLLAAGAKADVANRYGITPLLLACENGSEGI
                     VRALLKAGADPQAKRRGGETALMIASRTGRVGAVAALLEKGAEVDAQDREDQTALMWA
                     AAEGHAEVIELFVKHGAKVDHRLKSGFTAFLFAAREGKSAAVKKLLQLGADVHDAIDT
                     KEFTGGRDAPNGTSAVLLAAENGHFELAMDLIRAGADPNDMRNGFTALHTITWVRKPP
                     HGDDEAGQPPPDTHGKLSSLDFIREIVKAGAKVDATLAKVAKAKAFGAINFHGATPFL
                     LACRNADLEMMKLLVELGADPHKPNADGSTPLMAAAGLGCYAPDEEAGTEDECVAACN
                     YLISLGAEVNAVDNKNQTAMHGAANKSLPKVAQLLAAKGAKIAVWNQKNDRGWTPLLI
                     AQGFRPGNFKPSVPTITAISEIMRANGIEPPPPPDRDALPKKKGYEQK"
     gene            15442..16347
                     /locus_tag="PLP58_06145"
     CDS             15442..16347
                     /locus_tag="PLP58_06145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ08129.1"
                     /translation="MKLLSAFLCVLCGFTQAAEPLKLLFAGSSSMYWNDLPREIAKLV
                     DGKIAGHIGRQVIPEAVGRSGSDIRVYLEPGFNRYEYGVKPGQSFLDKIATEKPDIVP
                     MMVVCRFIMGDEAPTNGSPDHATSITRYSEAIRAAGGEPMFYEMGWGKTEREAEGRQR
                     ILELAKKNQITLFAPCSTAWSRVYAEKPDLALQHPQDNAHPGDAGHFLNLACFYAALT
                     RESPVGKLPRTFHVWPHGKYEADEAKLAAFQPDAYQGKMAKWIFKHMSMHATATLDDE
                     TACYLETVAWETWQAVSQTLLSGQK"
     gene            16441..17010
                     /locus_tag="PLP58_06150"
     CDS             16441..17010
                     /locus_tag="PLP58_06150"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HRJ08130.1"
                     /translation="MPLDHETILKHLFEARQRLSGVAWLMVRDAQAAEDLFQNVALKS
                     VTKNVAFEHEGALLSWAMVSIKRESIDWLRKRKPETLGLEPEVLDLISEEWSTHPQAE
                     GQRMEALRECIASVPEKSRQLLHLRYFDGHSCDEVARKVGANVDAVYQRLSRLHRQLK
                     QCVEQKVAAASAAFPASDAAKAATTFPSP"
     gene            17007..18284
                     /locus_tag="PLP58_06155"
     CDS             17007..18284
                     /locus_tag="PLP58_06155"
                     /inference="COORDINATES: protein motif:HMM:NF016648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FecR family protein"
                     /protein_id="HRJ08131.1"
                     /translation="MNDDLLIQRYLDGSISREDLAVLNQRLRDKADLRQHLCDISEQV
                     AAFGDMARQHRETDTPVCSPKRPDRGVWLTSLALAASIAVLAASAWLFFASKPDAVLT
                     LVESTGTVAWSDGTLIASGERLPAGTLETVGEASSALLRFDDGSLITLHGDAELSFAS
                     EHQKVLSLTRGTLSAEVRPQPAGQPMLIRTPSAVAEVVGTAFDLSTRSEDTLLKVNKG
                     LVKLKRLADGSEIDVPAHRSAVASLDTGAALDAASTPEPLTAWRFDFTSSTPPRDWRG
                     FSKNGAMHASPYVAKKMPDGRIVTHHGVSIRTAMLEQPLRLLATEHSVIKYRLRQERP
                     GQLQVMLLTNRTEGGFGGNFECKIPAEELRPGPDGWCEVAIPFTRYEPIDPRPHIRKR
                     HPSAVGNVITSAIISTFREDRHLEVSHFELGTR"
     gene            19163..19540
                     /locus_tag="PLP58_06160"
     CDS             19163..19540
                     /locus_tag="PLP58_06160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ08132.1"
                     /translation="MQASGKSYSIITDTADEAPWHPLSPVSLARLEPRPADWNYDVVQ
                     GALQPTFLARLTLKRLRDADEHLYGRDTLAEYAAWAAKIMHEKLHLNTETKEAINFAL
                     ERTPPENQKALLDLKAQVENGGQ"
     gene            complement(19636..21039)
                     /gene="ahcY"
                     /locus_tag="PLP58_06165"
     CDS             complement(19636..21039)
                     /gene="ahcY"
                     /locus_tag="PLP58_06165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018036870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004013 - adenosylhomocysteinase activity
                     [Evidence IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylhomocysteinase"
                     /protein_id="HRJ08133.1"
                     /translation="MSDYKVKDIALADFGRKELEIAEHEMPGLMATRQKYGPGKPLAG
                     VRVTGSLHMTIQTGVLIETLKELGADVRWASCNIFSTQDHAAAAIAASGTPVFAWKGE
                     TLEEYWWCTWQCIMFPGNKGPQLIVDDGGDVTLLIHKGYEMENGDKWVDSPSDNHEVK
                     VIKDLLKDIAKKQPGIFHEIVKDLKGVSEETTTGVHRLYEMAKAGKLLIPAINVNDSV
                     TKSKFDNLYGCRHSLTDGIFRAMDVMLSGKVSVVCGYGDVGKGCAQALKGQGSQVIVT
                     EIDPICALQAAMEGYRVMPIEDTLGIADVYVTTTGNKDIIRLEHMEAMKDQAIVCNIG
                     HFDNEIQVDKLNARTDVKRVNIKPQVDRYTFPKGNSIYMLAEGRLVNLGCATGHPSFV
                     MSNSFTNQCLAQMELWATKDSRPVGVTVLPKQLDEEVARLHLDKIGVKLTKLTKDQAD
                     YIGVPVDGPYKAEHYRY"
     gene            complement(21269..22666)
                     /gene="cysS"
                     /locus_tag="PLP58_06170"
     CDS             complement(21269..22666)
                     /gene="cysS"
                     /locus_tag="PLP58_06170"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012420072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004817 - cysteine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006423 - cysteinyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HRJ08134.1"
                     /translation="MPLTLHDTLTREARVVRALDGGTLKFYCCGPTVYGPAHIGNFRT
                     FVVQDVFRRVVERDGTATLHVRNITDVDDKTIRDSQKAGLSLADFTRHWTEKFHADCA
                     ALNLLPPHVEPGAVAHIPHQIRMIEKLIAKGHAYATSGGVYFKVSSFPQYGRLSHLED
                     REIKLGASTAATDSDEYDKDSLSDFALWKARKPEDGANFWRSPWGEGRPGWHLECSAM
                     SLEYLGEDFDVHSGGVDLVFPHHENEIAQSCCATHGRFAHHWFHVAHLMVDGGKMSKS
                     LGNLYTLEDLANRGYTPEEVRYVLISGHYRAPLNFTFHSLDAARHALQKMARFQKSAL
                     ERLAIHSVEPSGPGPFASAWETLNHDLNTPGCLGEVFTTLNRLKPAHLSDSEIVEALG
                     GLRFILQALGLKLPELKEDTATDVPTEVATLAARRWEAKKNKDWAAADILRKEIESAG
                     WIVKDGKDAYELTRK"
     gene            complement(22682..23017)
                     /locus_tag="PLP58_06175"
     CDS             complement(22682..23017)
                     /locus_tag="PLP58_06175"
                     /inference="COORDINATES: protein motif:HMM:NF024483.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HRJ08135.1"
                     /translation="MDAPEALREFLAYVVANLIDHPRQASIASGRNASGTLVFRVMLD
                     PDDMRRVHGRNGMTASAIRSLLSTAAEKHGLRVSLKISANKEQDDSAEPGEGAPPADG
                     DPDTNGGED"
     gene            complement(23087..23338)
                     /gene="rpsP"
                     /locus_tag="PLP58_06180"
     CDS             complement(23087..23338)
                     /gene="rpsP"
                     /locus_tag="PLP58_06180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018968725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HRJ08136.1"
                     /translation="MSVAIRLRQEGSKGRPIYRIVAADKRYRRDGRFLEILGSYDPAR
                     KDNKAEVNLERVDAWIANGAQPSDTVRSLINKARAAAQA"
     gene            23337..23717
                     /locus_tag="PLP58_06185"
     CDS             23337..23717
                     /locus_tag="PLP58_06185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ08137.1"
                     /translation="MDVIFLNAGFEGRANCGAAGGNQMVFIGFPGRHAAGISALFDDG
                     SFDRSFNIGCFHNRSGFRSGLFGGSFFGGSLFGRFGGGSFGSGRLARQTRLGQGFFAR
                     QVEFATTLFDDFVKLLAHDGVCVF"
     gene            23862..24599
                     /locus_tag="PLP58_06190"
     CDS             23862..24599
                     /locus_tag="PLP58_06190"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: protein motif:HMM:TIGR00671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004594 - pantothenate kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type III pantothenate kinase"
                     /protein_id="HRJ08138.1"
                     /translation="MNAEYLLIDVGNGRTKLALASPEALLDRRDCPTPGLDPGAVRAI
                     LNGWDFRRAVLCSVVPAAAAVFRGVLGEKMTELRHDTPMGIGVRYPCPESIGADRLAN
                     AAALAHLHGAPGVVIDFGTAVTFDILSADRHYIGGVIAPGLRLMTDYLHERTALLPKV
                     ELREPECAIGRSTVQAILAGAAIGYRGMIQGILDALRKELPQGPVHLVATGGDAGWIT
                     AGMAEPIIVDPDLTLHGLRLVGNLAAV"
     gene            24659..25597
                     /locus_tag="PLP58_06195"
     CDS             24659..25597
                     /locus_tag="PLP58_06195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009966012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HRJ08139.1"
                     /translation="MPTYTTSVGIDFGGTSVKLGVCRDGELLCTDAPIPTANFPGPAA
                     LIGEMAARVAKLKEKFPAIAAIGVGVPGLVDFEHGFVHVLTNVPGWKHVPLKAILGEK
                     TGLPVIVENDANAMAYAEFRYGAGRGMNNVVALTMGTGIGGGLILNGQLYRGSGCAAG
                     EIGQMSIHFDGVAGHYGNLGALEKYTGNQQIAEHAMRRYAEAHIQKSAEDCTPKKIAE
                     AAQAGDDIARQVWDEIADWLGTALASIAWLLNPDAFVIGGGVAQAGDLIFGPLNRKVQ
                     SMLSTVVWERLQILPARFSNESGIIGNAALAADSLG"
     gene            25697..26281
                     /gene="pyrE"
                     /locus_tag="PLP58_06200"
     CDS             25697..26281
                     /gene="pyrE"
                     /locus_tag="PLP58_06200"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012421133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004588 - orotate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HRJ08140.1"
                     /translation="MSLESDRAELAAILRAKSVKTGKFTLASGKESDLYVDCRVTTLD
                     ARGAVLVGRVLHDLLRREEAARGHSIGSLGGLTMGADPISLAVAMTSSLAGDASPVQA
                     FTVRKEPKGHGRGRRIEGNFTPHQPVVVVDDVITTGDSTLKAIQAIQEEGGSVAFALV
                     LVDRQEGGRANIESKACPVVAAFTREDLVGSANA"
     gene            26294..27214
                     /gene="rnhC"
                     /locus_tag="PLP58_06205"
     CDS             26294..27214
                     /gene="rnhC"
                     /locus_tag="PLP58_06205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009958360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HIII"
                     /protein_id="HRJ08141.1"
                     /translation="MPALSTYTKTLTTAQAEKLRGILQEEGWSFEDKAWCLYAASRGK
                     VNVLVYEKGPKAVVQGKETENFVLNTLEPRVLGVAELGYEEVHHPEFYQPHLGVDESG
                     KGDFFGPLVIAGVYADAGVARMLRECGAVDSKQISSDQKIEKLAEAVRAIPGLRWEVV
                     QINPERYNALYEKFGNLNRLLAWGHAKVIELLLEKVPDCPRAVSDQFANPAVLQRALQ
                     AKGRGIELVQRTKAESDPAVAAASILARERFVRWLRETGQSIGIALPKGVSPAVKESA
                     RRIVASRGADALTHFTKTHFRTTAELLAAS"
     gene            27268..27576
                     /locus_tag="PLP58_06210"
     CDS             27268..27576
                     /locus_tag="PLP58_06210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009958361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HRJ08142.1"
                     /translation="MANVTKRELVIELSNKLGLRQSEVMSMVEAFIEEVSATLERGDE
                     VTLRTFGTFELRKAKSKIGRNPKRPGSEVVIPERFVVRFKPGMELKQRVADLPVKDSK
                     "
     gene            complement(27590..28507)
                     /locus_tag="PLP58_06215"
     CDS             complement(27590..28507)
                     /locus_tag="PLP58_06215"
                     /inference="COORDINATES: protein motif:HMM:NF015431.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HRJ08143.1"
                     /translation="MRTQFELHSLEQFVALARARNFTRAAADLHLSQPALSRAIQKLE
                     DQLGQPLFERHPREVILTDLGELLLARAKEILKLVEDTFSELSEASRRGRIRLGAIPT
                     IAPYFLPGLLSGFAQKHPDISVVVQEDTTEILIQRCHHGEIDLAILALPIIAKGLEVA
                     PLFSEELLLVAPVGHPLAKVTPLTIQAVEDFPFVMLNEAHCLAENISSFCRQHTVQPV
                     TVERTSQLATVQELVALDHGVSIVPEMARRIDGSERRVYRSFHGEKPARTVAMMWNTA
                     RHESKASKAMKEYLLRKSKASQTAGKHGR"
     gene            28657..>29441
                     /locus_tag="PLP58_06220"
     CDS             28657..>29441
                     /locus_tag="PLP58_06220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009961773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ08144.1"
                     /translation="MKSLELRAVLATLMLTPVVFASAQDGPAKPNSDKPMDQLSKCPV
                     MGAPAPASRHTAASAMSNRDWWPNQLNLSILHQNSPKGNPLGEDFNYVEEFKKLDYDA
                     LKKDIQELLTTSQDWWPADYGHYGPLMIRMAWHSAGTYRVSDGRGGAGYGTQRFAPLN
                     SWPDNANLDKARRLLWPIKQKYGNQISWADLMILTGNVALESMGFKTLGFAGGRADVW
                     EPQEDIYWGPESEWLGDKRYKGERDLENPLAAVQMGLIYVNPEG"
BASE COUNT         5727 a         9184 c         8995 g         5535 t
ORIGIN      
        1 cacggcattg accagcgcgc cggcgcgccc ggcggcggcc acggtttcat tcacggcggc
       61 atgggcgggc acgccgagca gcaggagctg gcagaggccg gagcgcagcg acccgcgcgt
      121 gtgcgcgtcg aattttttgc cgccggtgag gtggctggcc caatggtcga gcagggagag
      181 attccgcagg caggtcagca cgatgtcgtg aagaaaggcc gcgttcgggc ctgagaggac
      241 ggcgcggcgc gcgcgctcgt ccagcagctc ggcggcaaag cgccccgtct tctcccagtc
      301 gagcagaacc tccagcgcca tgcggcggat gccgggaggc tggccgtggc gggcgcctgg
      361 gtctgctgtg ccggtctggg gtttcatccg cgctccgcga tgacgccgcg tccgatgccg
      421 cgatattgaa agccgaagtc ccgcgcggcg gcggggtcga gcaggttgcg cccgtcaaag
      481 accagcggcg tcatcatggc ctcgcgcagc tctgaaaggt caacgctggc aaactccgcc
      541 cactcggtgg cgatggccag cgcctcggcc cccttcgccg cctcaagcgg actggcggcg
      601 aatgtgacgg cggaggcaaa gtgcagcgcc ctggctttct ccatgccttt gggatcgtaa
      661 acggtgaccc gcgcgccctc ggccaccagc ctctccacca gcgccacggc caccgaggag
      721 cgcacgtcgt ccgtgttggg cttgaaggtc agcccccaga cgcagatctt tttgtccttg
      781 agcacccaga gcgcctcgcg catggcgtcg aggaagcgct caagctggcg ggcgttgatc
      841 ttctgcacct ccttgagcag atggaagggc acgccgagct gctcgctgat ggcgatgaag
      901 gcggcgatgt ccttcgggaa gcaggagccg ccgtagccga ggccggcatt gaggaagccg
      961 cgcccgatgc gcttgtccgc gccgatgccc tcggccactt tcaacacgtc cgcgccggat
     1021 gcctcgcaga tttccgcgag cgcgttcgcg tagctgatct tgagggcgag gaagctgttg
     1081 gcggcgtgct tgatgagctc ggcgctgttg atgtcggtga cgagcaccgg ggcgacgaag
     1141 ggctcgtaga ttttttgcat gagcgccagc gcgcggtcgc tgttgccgcc gatgacgatg
     1201 cggtcgggct tcatgagatc ctccaccgcg ctgccctcgc ggaggaactc gggattgctc
     1261 accacgtcga actcgacgcc gggtttcgca tagcggcgga tggtctgcgc cacgcgctcg
     1321 cccgttctca ccggcacggt gcttttgtcc accaccacgc ggtagctttc cagatactgg
     1381 gagatctccc gcgccacttt ctccatgaag ctcagatcca cgctgccgtc ctcctgcggc
     1441 ggggtcggca cggcgatgaa aagcacctcg ccatgatcca cgccctcctc cgtgctggtg
     1501 gtgaagcgca gccggcgcgc cgccacattc tttttcacca gggcctccag ccccggctcg
     1561 taaatgggta tctcccctgc cacgagtttc tcgatttttt tgggatcgtt gtccacgcac
     1621 acgacgtggt ggccgacttc ggcaaaacag gcccccgtgg tgaggccgac gtatcctgaa
     1681 ccaatgatgg tgagcttcat gcgtttgaaa gtcccggcct catggcggcc ggggacgccg
     1741 agcctagcac gcgcgccgcg tttcgaaatc cgaaatatca cgccgcgccc cggttttgtg
     1801 gtagatgggc gccggtcctc gttttctcac catgaaacac atctgcatcg ccctgctgct
     1861 cctggcccgg ccggccctct cgctggaccg gcccagcctg caaatcatca acgcggcgcc
     1921 ggacgccgtc gaagtcttct ggctggctcc ggacgggaag cgcgtgtcga acggcagggt
     1981 cgggccgggt gaggacaaga tcatcggcac caccctcggc caccgctttg tcatcgttga
     2041 cgggcggcgg gaaacggagg tggtgagcaa ggcgcgcgtc cagggtttcc gctacgatcc
     2101 ggcggccagg gatggcatcc cggcttttta cacccaggtg gttcatgccg ggggctatcc
     2161 catttgcgcc tcggaaaatg tgagtccgta tgcgctcaag gaggcggcct accttgtgga
     2221 cctcatgctg gcaaaacggc ccgatgtgcg cgaggcgatg atcgccagcg gatcgaggct
     2281 gtgcatcatc gcgcacaacg agttcaccac cggcctgccg gagttcgggc ggctggggcc
     2341 ggtgaagggc tacgaggagc tggcgccgca ggattactgg gatgcccgcg cgcgcggcac
     2401 cggcggcagc ctgacggatc ccttctgcac ctgcggggag gagaacctcc tggcttacga
     2461 gggcgatcct tacgacgcgg aaaacatcct catccacgag atcgcgcaca acatccatct
     2521 gcgcggcatg acgaatgtgg atccggcatt cgacggacgt ttgaagtcgg cctatgacga
     2581 ggcgatgaaa gccgggcttt ggaaggggct ttacgcctcg cgcaatcatc acgaatactt
     2641 cgccgagggt gtgcagagct ggttcgatga caaccgggag aacgaccacg accacaacca
     2701 tgtcaacaca cgcgcggagt tgacagccta cgatcccggc ctcgccgcca tgtgccgcga
     2761 ggtcttcggc gacaccgtcc tcaaatacac caaaccccgg acccgcctcg tcgcacatct
     2821 cgccggttac gaccccgcca aagcgccgaa gttcgagtgg ccggagcggc tcatcaaagc
     2881 ccgcgcattg ataaggaaaa aagccgtcga ggctcatgcg gcagccagcc tggaggaaaa
     2941 gacgcacgcc acgcgcgcca tcgcgggctg gaccgtgaac atgagcctgc aactgctccg
     3001 ggacgatgcg gtggcgctgg aaaaggcgct cactctcctg cgggcgcagc ttgatgagat
     3061 cgcgcgggtc gttccgcccg gggccgtggc cgagctgcgc aaagtgccgc tgtggatcaa
     3121 tcccgagtat cccggcgcgc gtccacgcgc cgagtatcat cccggcgcgg gctggctgcg
     3181 cgaaaacggg cgcgaccccg tgatggaaaa ggcggtggag ttcaccaacg tgcgcgtctt
     3241 cgagcaagag actcggcgca tgcccaactt cgcgctgcat gagctggcgc acgcctttca
     3301 tgaccgggtg ctcggctttg acaacgcgga gatcaaggcg gcctatgaaa aggccgcggc
     3361 ggcgggcggt tatgaaaagg tgcggcgccg ggacgccgag ggccgcatga ggctggacaa
     3421 ggcttacgcg atgaccaacg cgaaggaata cttcgccgaa tgcactgagg cgttcttttc
     3481 tcgaaatgac tttttcccct tcacgcgcga gcaactgcgg gcgcatgatc cggaaatgtt
     3541 cgcgctgctc ggaaagcttt ggggaaccag cgaaggctga gtgatggctt aacgcagccg
     3601 gtcttgccgg cgatactcgg tgggtgacag gccggtgacg gcccggaagg cccgcgtgaa
     3661 ggcgctgtga tccgcaaagc cgcactccag cgcgatctcg gcgatggagg tgttcgtttc
     3721 acgcagcagg cgggagccgg cggtgatgcg cgtcttcgtg atgagctgca tcgggctgag
     3781 gccgtggatg cgtttgacca cgcgggcgaa gcgcgcggcg ctcatgccgg ccttttttgc
     3841 cagcgacgac gggctcacgg gctggtcata cgatcgttcg aggtgccgca gcgccagcgc
     3901 cacggcgggc tgcacctcgt cccggctcac gagcgcggtg aggtcctttg aaatgccgat
     3961 gaggccggtc acgcggcccg tgccatcgcg gatgggaatc ttgctcgtca gaccccacac
     4021 gggcacattc ggcctgcgcc agaacaactc cagccgctcg atgatcggcc tgcccgtgcg
     4081 cagcacctgc tcatcctgct cggtggggat gcgtccataa tcacccggac acacatcgca
     4141 ggggcgccgg ccgaccattt gcgctttttc agccagccca tgccgctcca cgagcgactg
     4201 gttcaccacc aggtagcgtc ctgcggcatc cttgatgaaa aaggccgtgt catgcatcaa
     4261 atcggcgatc tgctcaacga gccgcgcgtc aatgagcaag cccccttttt gggaaaggat
     4321 gcccgagaac gctgctgccg cggccttcat gataagacag ggtgaacaac gaacatccgc
     4381 gccctgattt ccagccgttt tgagcaatga acagacgcaa ggccagccct ccgatgccgg
     4441 cctgttccaa aggctgctcc atgcctgtga aatgtcaggg ttggctgaac tcccgcgttg
     4501 cctgaagaca tgagatttcc gacgcttgtg atgatcctgc tgaccagctc cgcgatgggg
     4561 gcggattttc gtgggttttt ggaaacgcat tgcctggagt gccacgacag cgagatgaag
     4621 aagggcggcc tcgatttgtc ttcgttcata gatgaagcgg cggtgatgcg ggatcgctcg
     4681 atttggcggt cggtgtttga aaagattgag tcgcatcaga tgccgccgcc gaagcagaaa
     4741 tcgcagccga ccgaggcgca gcggcaagag ttgatgacat ggatcatgga catcgcggcg
     4801 cggccggatg cggacctggg agcggttgat cctggaaaac ctgcgctgcg gcggctgacg
     4861 cgtttggaat acaacaacgc gattcgcgac ctctttggcc tggagatgga tctcttcatg
     4921 tttccggagc ggttgcccat ccgcgacaag ggctacttcc agcctgcgaa tggcaagatg
     4981 ccggacgagc tcaaaatcga gatgcgcgag tatggggcga agtatccggt gctctgtccg
     5041 caactgggtc tgccaggtga caaccgcgcg gaacatggct accgcaatcg cggtgatgcg
     5101 caggatttct cgccgctgct gctggagaaa tacctcgcgg ccgcgctgga gatcgtgaac
     5161 gcgccagaac tgccgcgccg cagccgtgtg tttaccgaac ttgttggcat gaagcaggaa
     5221 atgcaggagc ctgcggacgt ctcatccaac gctccgactc ttgcgcccgt ccactcgccg
     5281 gacatcgaac gcgccgccaa agccgagggc agtcccgact atctggagcc cttccgggag
     5341 catgtggcct cggcgcacgc agacggccgc ggaggtgtct tcgatgtgcc gcgtgcgctc
     5401 gccaaccaga ccatcgccgg caaaggcggg ctgattcgtg cggccctcgg tgcccgcgcg
     5461 ctcaccatca atcccgacag cgatctctgg ctcgcttctt ttgcgaccac caagcccgcc
     5521 tcaactcctg cgatcctcac aaacaaggtg aaaggcgtga aaacctttga actgaccttc
     5581 gacatcgtca gcgaggatga cgatgagggc atcgagcgtc tcggcgtttg tgtcatggga
     5641 cggcaaaaac aaagcggcat ggtcaaactc accgctgtct ttaccgacgg caccgatgcc
     5701 gcgatcaccg ccttcatcgc tgaaggcgcg gcaggcacca cgttctattc cttcgccgca
     5761 ccgtctggtg aggccatcaa gaagctcgcg gtggatggtt cgcagttcag tggtgactat
     5821 gtgctgctcg acgatctcgg catcatcacc aatggcgcga cacagacact cctctctgtt
     5881 caaggccgcg aaccggcgaa gcctgagaaa aaggaaggac agacaagagt gtctgtttca
     5941 ctcactcccc acgaacggat tgaggcattc atgtctcgtg cgtttcgtcg cgaggtggcc
     6001 gatgaagagc ttgcccccat tttttcgatc ttccaaaacg cacgaaaaag cggccggtca
     6061 gaggcggatg ccatgagaac cgccatcgcc gccgtcctgt cctcgccggg ctttctctac
     6121 gttgaggcca atggcgacgc gacggccggt aaggtttcac ggctggagga tcatgagctg
     6181 ggcacccgtc tcgctctctt tctctggtca tccacacccg atgacaaact gctcgctctc
     6241 gccaaagcag gcaaactccg agacaacctc gaaaaccaag tccgccgcat gctcaaagaa
     6301 cggcgctcgc gcgagctgag cgagtcgttt gccgtgcaat ggctgcggct tgatcagctc
     6361 cacacttcca aacccgaccg tgatctgttt ccgggcttct actcaggccc tcagggaaaa
     6421 tccacacttc atggagcgac actggccgag gcgttgcttc ttttcgaaac ggttctcgtg
     6481 gaggaccgcg gcatccttga cttcctcgcc gcagattaca catggctgaa tgcgccgctc
     6541 gcccggcttt acggacttca gttcagcgct gaggatcacg cgcccgaagt ggcaaccaac
     6601 acgaaccgcg aattgaagac gaagaacgac aaggccgccc tctggcatcg cgtcaagctg
     6661 agcgatgcaa atcgcggcgg cttcatgagc atggccgctc ccttggtcgt cacgtccctg
     6721 cccttccgca cgagtccggt gaagcgcggt gcctggatgc tggagacact tttcaaccgc
     6781 ccacccaccg agcccaaggt ggcctttgcc atcgagaacg acaccaagga agccgcgcag
     6841 cagatgagca tccgcgagaa gttcgaagcg catcgcaaca aggccgcgtg ctactcctgc
     6901 cacatccgcc tggatccgcc cggcttcgca ctggagcgct tcaaccccat cgggcagtgg
     6961 gatgaaaaag cggatacgaa gggcgaatgg tccggcagcg cctttgacgg ccccgcaggc
     7021 ttcaagagca ttctcgcccg ccatccccat gagtttacga gaggtttcat cgagcacctc
     7081 ctcagctacg cgcttggccg aaagctggaa gtttatgaca tgcccgtggt ggaccacatc
     7141 cagcgcgccg ccgcggctga tggctggaag ttcagccgta tcattgtcga gatcgtccga
     7201 tcctatccgt tcacccatgt gcgccgctca tgatgaatcg ccgccatttt ctccgctcca
     7261 ccggcatcac gctcggcctg ccgctttttg aaagccttgc caaggccgcg ccagcagcct
     7321 caccgcgccg cttcgccttc atctacacgc cgaacggcta cctgcagagc aaactgcttc
     7381 cgcaaaaagc cgggcaaggc ctgaacccgc tgactctgac atccacgctg gagcccctct
     7441 caaagatcaa agacgatctc accttcatca ccggcctgga ccgccagttt gtgccgggaa
     7501 cgggtgttca cgcgcaggca ggctcgtgct ggctaacttc atcggcgcca caggaaaccc
     7561 tcgatggagg cttcccgacg aatctcacgc tcgaccagct catcgcccgc cagatcggcg
     7621 cggacacggc cctgccttcg ctggagctca gcacgaacga cttcaccgac aacaaagaga
     7681 cgaaatactt cgaatgcatc tcgtggtatg gccccggcta cgcggcgaac acggaaaaga
     7741 acccgcgagc cgtgtttcag cgtctctttg gtcaaggcca gggcggcccg caacgcagca
     7801 tcctcgacgc ggtgctcgcg caggcacaca gcctcgagaa acgtctcggc ggtgaggaca
     7861 aacaaaagct cggtgagtat ctcgaaagtg tccgcagcac cgagcagcgc atccaacgcg
     7921 ccggggcagc cgcccaacgc atcggcaggc cagccttggc agaaccgaag ggcatcccgg
     7981 aaaagcgcag cgagtatttg aagctcatgg ccgagctgtt catccacgct tttcaaaatg
     8041 acctcacccg cgtcgccacc ctgctcgtcg caccggagcg ctgggacaat cccacgctct
     8101 acgacggcct tttcgacaaa ccgcagaacc accacgtcct gacccatacc aagggcgatg
     8161 aagccaagga caagctcgcc ctcattgacc gctatcacat ggagttctac gcacacgtcg
     8221 tccagcgtct caaagccacc agcctgctcg aaagcaccac gctcgtcatg ggcagcggca
     8281 tcagcgatgg cgaccagcac aactacgccg acctccaggt cttcatcgcc ggaggcggct
     8341 ggaagcgcgg ccatttccac tacgaaggca aacgtccgct ggccgatctc tggctcacga
     8401 tggcgcaaca agccggcgtg aagcaggaac gctttgcgga cagcacgggc atcttgaccg
     8461 agatatgacc atgagtttaa ccacagagac accgagcaca cagagaacca cacagaagaa
     8521 tcctctgtgt gctccgtgcc tctgtggttc aaaagcgaag ctcacgaatc tcttctctgc
     8581 gtttctctgc gccctctgcg gtttcctcca tgccgcgcag ccaccgcgtg ccgcgctgga
     8641 gcgtttctgc atcgactgcc atgatgcaga gacgaagaaa ggcggtgtgg atctcgattc
     8701 gatcctccaa gatggcatcg cgaaacatcc ggccacttgg gaaaaggtgg tgaagcagct
     8761 caacgcccgt cacatgccgc ccatcggcaa aaagcggccg gatgaagcgg gttacgaggc
     8821 gctcatcacc gcgttgacga cggaactcga cccccagccg cccaaagcca tcgcaggccc
     8881 ggcatttcgt cgtctcacgc gcacggagta tcaaaatgcc gtgcgtgacc tgctggcggt
     8941 cgagtttgat gcgaaggcgg cctttccgat ggatgagatc agccacggct ttgacaacat
     9001 cactgtcggc gatttgccgc ctgctttgct cgaccgctac atcagcgcgg cgcaaaagat
     9061 cgcgcgacaa gcggtgggca cttcgacgca gccggagatc accgccatcc gcgtgcgacc
     9121 cgacatcacg caggaatatc acgtgcccgg attgccgctc ggcacacgcg gcggcgtgct
     9181 ggtgaagcat gtctttgcgc aaagcggtga ctacgaggtg catgtgcgcc tccagcggga
     9241 tcgcaacgag caagtcgaag gcatgcgcgg ccagcatcag ctccaaatcc tgctcgaccg
     9301 cgaagtgcag agcgagttca ccgtccaacc ggccaaggac aaaaactacg agaagatcga
     9361 cgcgcatctc aaagcccgct ttcacgtcgc cggcggccag catgacctcg gtgtgacctt
     9421 cttgccgaat gggtcgcccg tgctcgaacg tctgcgccag ccctacaagt cgagctacaa
     9481 tctgcatcgc catccgcgtc tgaatcccgc cgtgtatcag gtcaccatca ccggcccgct
     9541 caaggccgag ggcgcgggcg acacgccaag ccgtcgtttg atcttccgag cgagtaaaac
     9601 agaccccgag gccatcttga ggccgatttt gcgccgcgca tggcgacgtg acatcacggc
     9661 gactgatctt gagcgcatgc ttccattcgt tcgcgaggct ccctcactcg aaaaaggcat
     9721 cgaatcggct ctcgcggcga ttttggtcag tcgcgagttt ttgttccgca cggagcaaaa
     9781 cggcgatgaa gccgctttgg cctccaaact ggcgtttttc ctttggagca gccttcccga
     9841 tgacgaactg ctctcgcagc gcccgctcga tctcgcagcg caggcccgcc gcatgctgcg
     9901 cgatccgcgt gcgaaggcgc tcgtgaccaa cttcgccgat caatggctct acctgcgcaa
     9961 tctcgatgcc atcacgccgg acgcgaggct gtttcccgac ttcgatcaca acctccgcga
    10021 atcgctgcgc acggagacga gcctgctctt cgccgatgtg atcgaaaacg accgcagcgt
    10081 gctcgatctg ctgcgcaccg accaaacctg gctcgatgag cgactcgccg cgcactacgg
    10141 catcccgcac atccgcggca gccgttttcg cgaggtgaaa ctcgactcgc caacgcaacg
    10201 cggcggcctg ctgcgtcagg gcagcatcct caccgtcacc agctacgcca cgcgcacctc
    10261 gccggtgatt cgcgggcatt ggatcttgaa gaacctgctc gccaccgagc ccccaccgcc
    10321 gccgccaaac gtgcccgccc tcgatggcgt catctccgaa agcctcccca tccgcgagcg
    10381 acttgccaag catcgcgaaa tgaccgcctg tgccagttgc cacaacctca tggaccccgt
    10441 cggcttcgcc ttggaaaact acgacgccat cggccgctgg cgtgcggaag ccgatgcccg
    10501 cggcggtttc gccgatggca gcgagttcga aggcgtgagc ggcctcgaag acaccctcat
    10561 gaagcgcccc gaactcttcg tcaccgcttt gacggaaaaa ctcattgtct atgccctcgg
    10621 tcgcggcctc gaagccagcg atatgcccgc cgtgcggaag atcgtgagcc gggcgaaggc
    10681 gaatggctgg aagttcagcg aaatcattat cggcatcgtg acgagcactc cgtttgccgc
    10741 atcaaagtga cacagatact cctgtctgtg tttctcagac ggcagacagc aactgtgtcc
    10801 gcctcacacc cagtcctcgc aaggatcgat gcggccttcg cgctcatcag ccattttcca
    10861 gtgaaacggc agtccgtgac tcccatgcgt gaccggttcc cagccgaagc gggatcgaaa
    10921 agtatggtaa gccggcgagt cccgcagagg aatccagtca aacacatcct tccatccggc
    10981 aaacagcttc cagtcgtctc gctcgggaaa aacaccgaag tcaaatgccc cgcagatccg
    11041 atgcacatag tcagcccggt ccgccatggt gtgaagctcc agtggcggct cggcgagatc
    11101 agggtaaaca gagtgatcga gattcatcgg ggaccggctg ggcaaaatac atcccgatac
    11161 cagctgcgca aggctgccgc ctgagcacgg gactcggctg gcaacttcga cacgggccgc
    11221 atgagttcca gcttctccac ataaaacaga acctttcccg gcccctcggg tgggtcgcgg
    11281 tgggtgttct ttgcctgcca catggcccag cactcttcga tggtggtaat gctctgcgtg
    11341 cacagttggt ggatgtctcg aagatcacgc ggtgcaccac gctcgaccag cgctgtcatc
    11401 ttcgacgcca cattgtcccg cagcgtctca atccacagcg gctgccattc cgccggcaac
    11461 ggttcatcga ggtaaaggcc gcgcttgctg atctggaatg aaaactcctt ctggccgcct
    11521 cgcatgagtt ccagactttc ggtttctccc caagttctcc gccggtagtc gaggccgtgc
    11581 cttgcggcga gagacttcat ggccccctcg acaaaggcga gaacctcggg ccggggcgac
    11641 tcgcgccacc aagcatccag atcgaccgtt tcgcgatatt caaggtagtg gctcagcgcc
    11701 acgccaccac cgagcacgat ttccgccgcc tccgcatgac ccgcgaggct ctccagcagc
    11761 tcgcgggaga agggattcag gttttgcggg ggcttgggac ggatgttctg catcgccggg
    11821 cagtttgccc ggcatgattg cctttctcaa gagcgggctt tgtgtcagtt tcccctcctc
    11881 aacaccgttt cacccgtcat gagcaccgtt accacacgca aggccctctc ccgccgccac
    11941 ctgctgcgcg gcgtgggggc gacgttatcg ctgccgtttc tggaggcgat gatcccggct
    12001 gccacggcgg cgaatcgggt ggcgcagcca gcgaaacgac tgagctacat ctacatcccg
    12061 atgggcacgg accactcgcg gtggaatctc ggcgcgacga gcacgctgga gcagctatcg
    12121 cccattttga agtcgctgga gccggtgcgg cagcattgcg cggtcgtttc gaacctcgaa
    12181 ctcgagcctg cctatcccgg aacgcatgcc acgtcgaacg cgagcttcct tagtgccgcc
    12241 cgcgtgaagc acacggagag cagcgactac tacaacggca ccaccgccga ccagatcgcc
    12301 gcgaaggcca tcgggcagca gacgcaactg ccgtcgctcg agttggcgat ggacttgatg
    12361 caggtcgtcg gccagtgcga caacggctac gcctgcgtgt atcaaaacaa tctctcctgg
    12421 tcctcgccca ccacgccgct gccagccgag gcgcatccgc ggctggtgtt tgagcagctc
    12481 tttggcgatg gcggcagcaa aacagatcgt caggccgcat tggagcgacg ggcgagcttg
    12541 ctcgacttca tcaaggacga catgcagcgc ctcgcgaagc agctcggccc gcaggatcgc
    12601 gatcgcgtga atcaatacct cgacagcatc cgcgaggtcg agcgccgcat cgagaaagcg
    12661 caggccgccg tcgcggagaa cgagctgccg gacctctcgc ggcccagcgg cgtgcctgcg
    12721 agctacgcgg agcatgcgcg gctcatgttt gacctccagc tcctcgccat gcagggcgat
    12781 gtcacgcgcg tcatcacctt ccagctcgcc cgcgagacct cgaaccgcac ctatcccgag
    12841 atcggcgtca gcgatccgca tcatccgctc agccaccacg gcaacgatcc cgcgaagatc
    12901 gagcgcatgt cgaagatcaa tgccttccac gtcagcctct tcgccgaata cctcgccaaa
    12961 ctcgccgccg tgaaggaagg cgacggcacg ctgctcgacc acacgctgct cctctatggc
    13021 agcggcatgg gcaaccccaa cgtccacgac cacaccaacc tccccatcat cgtcgccggt
    13081 ggtgccagcg ctggagtcaa aggcaaccgc catctgcgaa acaaccaaac catcccgctc
    13141 gcgaatctgc atctcacgct gctcgacaag gctggcgtga agctagacaa gttcggtgac
    13201 agcagcggaa tcattgaggt gctgtgattt actctcatgc gtcccctttc tgaactcatt
    13261 aaccctgatg attcagcact tcccattatc gaggagttag ctgcaaaggt ctcaggacgc
    13321 tgtgttgtgt tgccgccaag acctgatagc ggcgagatac tgtatcgaac acaagtcacc
    13381 acacgatccc cgatgggtgc cgtagtctac cacacagggg gcattgtggt ggatcgctgg
    13441 attcggatca ttggctcagg atcatcaatc atcgaacgca atctgccaga ttggaactca
    13501 agccgggcaa atggcttcta tttgattggc gacgatgtcg ttggcggatt tttcgccatt
    13561 gatggcggtg ggctgggatt tgaaaaggga catgtctgtt attgggcgcc tgactcactc
    13621 gagtggattt cgatggagcg tggctacaca gactttcttg tctggtccat gaccatggac
    13681 acgacggact tttatggtga ccttcgctgg tcgagttggg aagcagaggt cacttcggtt
    13741 ccgggagatc ggtgcttcgg gttctatccg tttctttgga ctgtggaagg ttcagcttcc
    13801 tcgagcaaac gagcgatagt caccgtggag gaagcattcg atatgaagat ggacatctgc
    13861 cgacagcttt ctgaaccatg agtcttccac ttctgcctat cgaactggct ctgctcactt
    13921 tgtttttcgg aaacctagtg gccaccgatc ttcccaccgc cgccgaaaag caggactgga
    13981 actccgtttc ggctcaaatc gcctccaagt ccgatttgaa tgccacgcaa gccgatggag
    14041 caacggcgct gcattgggcg gcttaccacg acaaagctga gatcgtcgaa aaactgctcg
    14101 ccgcgggcgc gaaggccgat gtggcgaatc gctacggtat cacgccattg ctgctcgcgt
    14161 gtgaaaatgg cagcgaaggc atcgtgcggg cgctgttgaa ggcgggagcc gatccgcagg
    14221 cgaaacgacg tggcggcgag acggcactca tgattgccag ccgcacgggt cgagtcggcg
    14281 cggtggcggc cttgctcgaa aaaggtgcgg aagtggatgc gcaggatcgt gaggatcaaa
    14341 ccgcgctcat gtgggccgcg gcggaaggtc atgcggaggt catcgagttg tttgtgaagc
    14401 atggcgcgaa ggtcgatcat cggctcaaat cgggcttcac ggcttttttg ttcgccgcac
    14461 gcgagggaaa gagcgcggcg gtgaagaagc tgcttcaact cggtgccgat gtgcatgacg
    14521 ccatcgatac gaaagagttc accggaggcc gcgacgcgcc gaatggcacc agcgcggtgc
    14581 ttttggccgc ggagaatggc cacttcgagc tggcgatgga tctcatccgc gccggagccg
    14641 atccgaatga catgcgcaac ggcttcaccg cgctgcacac gatcacctgg gtgcgcaagc
    14701 cgccgcatgg cgatgacgag gccggccagc cgccgccgga cacgcatggc aaactgagca
    14761 gcctcgattt catccgcgag atcgtaaagg cgggcgccaa ggtcgatgcc acgctcgcca
    14821 aagtagccaa ggcaaaggcg tttggcgcga tcaacttcca cggagccacg ccttttctgc
    14881 tcgcctgccg aaacgccgat ctggaaatga tgaagctgct cgtcgaactc ggcgcggacc
    14941 cgcacaagcc caacgccgac ggctccacgc cgctcatggc cgctgcgggt ctcggctgct
    15001 acgcgcccga tgaagaagcc ggaacggaag atgaatgcgt ggcggcttgc aattacctca
    15061 tcagcctcgg cgcggaagtg aatgcggtgg acaataagaa ccagaccgcc atgcacggcg
    15121 cggccaataa aagcctgccg aaggtcgccc aactccttgc cgcgaaaggc gcgaagattg
    15181 cggtgtggaa tcagaagaac gaccgcggct ggacgccgct gctcatcgcg caaggcttcc
    15241 gacctggaaa cttcaaaccc agcgtgccca ccatcaccgc catcagcgag atcatgcgcg
    15301 cgaatggcat cgagccgcca ccaccgccgg atcgcgatgc tttgccgaag aagaagggct
    15361 atgagcaaaa gtagtttggg ctttagccca agctcacccc acaactattt gggctaaagc
    15421 ccaatctact tttcccacac catgaaactc ctctccgcgt tcctctgcgt cctctgcggt
    15481 ttcacccaag ccgccgagcc tctcaagctg ctcttcgccg gcagcagcag catgtattgg
    15541 aacgatctgc cgcgtgagat cgcaaagctc gtggatggca aaatcgccgg tcatatcggc
    15601 cgacaggtca ttcccgaagc cgtgggccgc agtggcagtg acatccgcgt gtatctggag
    15661 cccggattca accgctacga atacggcgtg aagcctggac agagcttcct cgacaagatc
    15721 gccaccgaga agcccgacat cgtgccgatg atggtcgtgt gtcgtttcat catgggtgac
    15781 gaagcgccga cgaatggcag tcccgaccac gccacctcca tcacccgcta cagcgaggcc
    15841 atccgcgccg ctggaggcga gccgatgttt tatgagatgg gctggggcaa aaccgaacgc
    15901 gaggccgagg gccgtcagcg catcctcgaa ctcgcgaaga agaaccaaat caccctcttc
    15961 gcgccctgct ccacggcttg gtcacgtgtc tatgccgaga agcccgatct cgcccttcag
    16021 catccgcagg acaacgcgca ccccggcgat gcaggccact tcttgaacct cgcctgcttt
    16081 tacgccgcgc tcacgcgtga atcgcccgtc ggcaaactgc cgcgcacctt ccacgtctgg
    16141 ccccacggca agtatgaggc cgatgaagcc aaactcgccg ccttccagcc cgatgcctac
    16201 caagggaaga tggcgaagtg gattttcaaa cacatgtcca tgcacgccac ggccactttg
    16261 gacgatgaaa cggcctgtta cctcgaaacc gtggcttggg agacttggca ggccgtgagc
    16321 cagacactcc tgtctggcca aaagtgatgc tggcactcct gtctgcattc tgggagacaa
    16381 acagacaaga gcgcctgttt cactgtcagg ccgcgctgac ttcatccgtt gcccagagac
    16441 atgccactcg atcacgaaac catcttgaaa cacctctttg aggccaggca gcggctcagc
    16501 ggggtggcgt ggctcatggt gcgggatgcg caggcggcgg aggatctgtt tcaaaacgtc
    16561 gcgctgaagt cggtgacgaa gaatgtggcc ttcgagcacg agggcgcgct gctttcgtgg
    16621 gcgatggtct cgatcaagcg cgagtccatc gactggctgc gcaagcgcaa gcccgagaca
    16681 ctcggattgg agccggaggt gctcgatttg atcagcgaag agtggtccac gcatccgcag
    16741 gcggaggggc agcgcatgga ggcgctgcgc gagtgcatcg cttccgtgcc ggagaagtcg
    16801 cggcagcttc tgcatctgcg ctactttgac gggcattcgt gtgatgaggt cgctcggaag
    16861 gtcggcgcga atgtcgatgc ggtttatcaa cggctctcgc ggctgcatcg gcagctcaag
    16921 cagtgtgtgg agcaaaaagt ggctgcggct tctgccgcat tcccggcatc tgatgcggct
    16981 aaagccgcaa ccactttccc aagcccatga acgacgatct tctcatccaa cgctaccttg
    17041 atggcagcat ctcgcgtgag gacctcgcgg tgctgaatca gcgcctgcgt gacaaagcgg
    17101 acttgcgcca gcatttgtgc gacatctcgg agcaagtggc agccttcgga gacatggcac
    17161 ggcaacatcg tgaaacagac actcctgtct gttcccccaa acggccagac aggggtgtct
    17221 ggctcacttc tctcgccctc gctgcctcca tcgccgtgct ggcggcgagt gcgtggcttt
    17281 tcttcgccag caagccagac gccgtgctga cgctcgtcga aagcaccggc acggtcgcat
    17341 ggagcgatgg cacgcttatc gcgagcggtg agagattgcc cgcgggcacg ttggagacgg
    17401 tgggcgaggc cagcagcgcg ttgctgcgct ttgacgatgg atcgctcatc acgctgcatg
    17461 gcgacgcgga gctttctttt gccagcgagc atcaaaaagt gctctcgctc acccgcggca
    17521 cgctttccgc cgaggtgcga ccgcagcctg cgggccaacc gatgctcatc cgcacgccca
    17581 gcgccgtggc ggaggtcgtt ggcaccgcgt ttgacctttc cacgaggtcg gaagacacgc
    17641 tgctgaaggt caacaaaggc ctcgtgaaac tgaaacgcct cgccgatggc agcgagatcg
    17701 acgtgccggc ccatcgcagc gcggtggcct cgctggacac gggcgcggcg ctcgacgcgg
    17761 cctccacgcc ggagccgctg accgcgtggc gctttgattt caccagcagc acgccgccac
    17821 gcgactggcg cggcttttcc aaaaacggcg cgatgcacgc cagtccctac gtcgccaaaa
    17881 aaatgcctga tggccgcatc gtcacccatc acggcgtctc catccgcacc gcaatgctgg
    17941 agcagccgct gcgcctgctc gccactgagc acagcgtcat taaataccgc ttgcggcagg
    18001 agcgtccggg ccaactccag gtcatgctgc tgacgaatcg aacggaaggt ggttttggcg
    18061 gcaacttcga gtgcaaaatc cccgccgagg aactccgtcc cggcccggat ggctggtgcg
    18121 aggtcgccat tccgttcact cgctacgagc ccatcgatcc gcgtccgcac attcgcaaac
    18181 gccatccctc cgccgtgggc aacgtcatca cctctgccat catcagcacc ttccgcgagg
    18241 accgccattt ggaggtcagc cacttcgagc tcggcacgcg ctgatttttt aaccacaaag
    18301 gcacggagca caccgagatg aagaaacagg tttcgatcct ctgtgtgctc ggtgcctttg
    18361 tgggttcact ccacgcggct cccgcgactt ttgactcctt ctgcttcaaa tgccacgatg
    18421 cgacccaaag cgacggcgat ctcgatttgg aggctttgac ggctcggaag gccgatccgg
    18481 cgcagtggcc tgttttgaga agcggcgtca ggcaagttag tcagcgaaag cgtctgtggc
    18541 gactctgtgc gaagtgtgtg tttcagtcct gatgggcgct ggctcgcggt ctccagtatg
    18601 gacaaaaccg tgcaggggtt gaagtggcaa cgggtaaaga tgtcagcaag accgtcgttg
    18661 gcgttaccgt gaaaacaatg tgtttcaacc gcgatggacg attgctggtg gttgcgacgg
    18721 gtagtttgga tggggacgag ggcgaattga gggtgattga agcagcgacg ggcaaggagg
    18781 tcggtaggac cgcgttcggc gatgcagcga aggtcgtgag tttcagcccc gatgggcgct
    18841 ggctcgagta gtaggttatg ataccatcag ggtgatcgac tgcttgtggc tgaatgttcc
    18901 tgcacaagga agcgaacctt ggcgggaaat gctgcgcagg caggccaggc ggcacttcca
    18961 agccgacgga agattggtgc cgttggccac gcaggattcc aaaggcgctc aggaagccat
    19021 tgctcgcttc ctgcaagaac cccctcccgc agatcgccat tgggaacatg acattcttac
    19081 atgggcacaa cagcttcctg acgtgcgtcc tacgtcgcct tggaccacag aacctctatg
    19141 tatcgcaacg ggacattggt tcatgcaagc ctcgggcaag tcctactcca tcatcacaga
    19201 cactgccgac gaagccccct ggcatccgct ctcgcctgtc tcacttgcac gtttggagcc
    19261 aagacccgca gactggaact acgatgtcgt acaaggggcc ttgcagccca ccttcctagc
    19321 ccgtctcacc ctgaaacgcc tgcgagacgc tgatgagcat ctctatggga gggacacact
    19381 ggcggagtat gccgcctggg ctgcgaagat catgcacgag aaactgcacc tcaacaccga
    19441 gacgaaggaa gcgatcaact ttgccctcga acgcaccccg ccggagaacc agaaggcgtt
    19501 gctggacctg aaggcgcagg ttgagaacgg cgggcaatga aagccataac gcgcccggcc
    19561 tttcgaccgg acgcgttgtg gggttttggg gatggtgagt gtcggaggct catctcgcgg
    19621 agcgagatgg ctactttaat accggtagtg ctcagccttg taggggccat cgacgggcac
    19681 gccgatgtag tcggcctggt ccttcgtgag cttggtgagc ttcacgccga tcttgtcgag
    19741 gtggaggcgg gcgacttctt cgtcgagctg cttcggcagg acggtgacgc cgacggggcg
    19801 gctgtccttg gtggcccaga gttccatctg cgcgaggcac tggttggtga agctgttgct
    19861 catcacgaag ctcgggtggc cggtggcgca gccgaggttc acgaggcggc cctcggccag
    19921 catgtagatg ctgttgcctt tcgggaaggt gtagcggtcc acctgcggct tgatgttgac
    19981 gcgcttcacg tccgtgcggg cgttgagctt gtccacctgg atctcgttgt cgaagtggcc
    20041 gatgttgcac acgatggcct ggtccttcat ggcttccatg tgctcgaggc ggatgatgtc
    20101 cttgttgccg gtggtggtga catagacatc ggcgatgccg agggtgtcct cgatcggcat
    20161 gacgcggtag ccttccatgg cggcctgcag ggcgcagatg gggtcgattt cggtgacgat
    20221 gacctgggaa ccctggcctt tgagcgcctg ggcgcagcct tttcccacgt cgccataacc
    20281 gcagaccacg gagaccttgc cggaaagcat gacgtccatg gcgcggaaga tgccgtccgt
    20341 gagggagtgg cggcagccgt agaggttgtc gaacttggac ttggtgacgc tgtcgttgac
    20401 gttgatcgcc gggatcagca gcttgcccgc cttggccatt tcatagaggc gatgcacgcc
    20461 ggtggtggtc tcttcggaga cgcccttcag gtccttgacg atctcgtgga agatgccggg
    20521 ctgtttcttg gcgatgtcct tgagcaggtc cttgatgacc ttgacctcgt ggttgtcaga
    20581 gggggaatcc acccacttgt cgccgttctc catctcatag cccttgtgga tgagcagggt
    20641 cacgtcgccg ccgtcgtcca cgatgagctg cgggcctttg ttgccgggga acatgatgca
    20701 ctgccaggta caccaccagt attcttccag cgtctcgccc ttccatgcga acaccggggt
    20761 tccggaggcg gcgatggcgg cggcggcgtg gtcctgggtg gagaagatgt tgcaggaggc
    20821 ccagcgcacg tcggcaccga gttccttcag ggtctcgatc agcacgccgg tctggatggt
    20881 catgtgcagg gagccggtga cgcggacgcc cgcaagcggc ttgcccgggc catacttctg
    20941 gcgggtggcc atgagaccgg gcatttcgtg ctcggcgatc tcgagttctt tgcggccgaa
    21001 atcggcgagg gcgatgtctt tgactttgta gtcgctcata gtgggcggat gtgggttcag
    21061 gtttggggtc tgaaacgatt caaatcaacg tatcaggatg cgttgatatg tttcaggatg
    21121 ccccgtgagt caaacgggga tttcagcgcg aacccggttt gtcagcgcat gagggcggag
    21181 ttcactcccc ggcaaactgg cagtctgcct tcgagagtgc cgccggactt gcctggatct
    21241 tcgagtcccg cgtgcgagac cgcacggctc acttgcgcgt gagttcgtag gcatccttgc
    21301 catccttgac gatccacccc gcgctttcga tctctttgcg caggatgtcg gcggcggccc
    21361 agtccttgtt ttttttggcc tcccaacgcc gcgcggccag agtggcgacc tcagtcggaa
    21421 cgtcggtcgc cgtgtcttct ttgagttcgg gaagtttgag tcccagagcc tgaagaatga
    21481 aacgcagtcc gcccagagct tcgacaattt ccgaatcgga gagatgggct ggcttgagac
    21541 ggttcagggt ggtgaaaacc tcgcccaggc agcctggtgt gttgaggtcg tgattgagtg
    21601 tttcccaggc ggaggcaaac ggtcccggtc cagacggctc gacggaatgg atggctagcc
    21661 gctcaagcgc gcttttctga aagcgggcca ttttttgcag cgcatgccgc gcggcgtcga
    21721 gggagtggaa ggtgaagttc aatggcgcgc ggtaatgacc gctgatgagc acataacgca
    21781 cctcctcagg cgtgtatcca cggtttgcca aatcctccag ggtgtaaaga ttgcccaggc
    21841 tcttgctcat cttgccgcca tccaccatca gatgagccac atgaaaccaa tgatgggcga
    21901 agcgtccatg agtggcgcag caactctggg caatctcgtt ttcgtgatga ggaaagacaa
    21961 ggtccacacc gccgctgtgc acatcgaaat cctcgccgag gtattcgagg gacatggcgc
    22021 tgcattcaag gtgccagccg ggccgaccct cgccccaggg gctgcgccaa aaatttgcgc
    22081 catcctcggg tttgcgcgcc ttccaaaggg cgaaatcgga cagtgaatcc ttgtcgtatt
    22141 catcgctgtc agtggcggcg gtcgaggcgc cgagcttgat ctcccggtcc tccagatgcg
    22201 agagccgccc gtattggggg aaggatgaga ctttgaaata aactccgcct gaggtcgcgt
    22261 aggcatggcc cttggcgatg agcttctcga tcatgcggat ctggtgcggt atgtgggcca
    22321 cggctccagg ttcgacatgc ggaggcagca gattcagcgc ggcgcaatcc gcatggaact
    22381 tttccgtcca atgacgcgtg aaatccgcga gactcagccc ggccttttgc gagtcgcgga
    22441 tggtcttgtc atccacgtcg gtgatgttgc ggacatgaag ggtggcagtg ccgtcgcgct
    22501 cgaccacgcg gcggaagaca tcctgcacga caaaggtgcg gaagttgcca atgtgggccg
    22561 gcccatagac agtggggccg cagcagtaaa acttcagcgt accgccatca agcgcgcgca
    22621 cgacgcgcgc ctcgcgtgtg agggtgtcgt gcagtgtcag cggcatggcg gcggatgcgg
    22681 gttagtcttc gccgccattt gtgtcagggt ccccgtccgc aggcggggcg ccctcccccg
    22741 gttcggcgga gtcgtcctgc tccttgttgg cggagatttt cagactgaca cgcaacccgt
    22801 gtttttcagc cgcagtggag agcagcgagc gtatggcact ggcggtcatg ccgtttctgc
    22861 cgtggaccct gcgcatgtcg tcagggtcga gcatgactcg gaaaacgagg gtgcctgagg
    22921 cgttgcgccc gcttgctatg gatgcctggc gggggtggtc aatcagattg gccaccacat
    22981 aagccaggaa ttctcgcaat gcttcgggcg cgtccatgga attgcttcaa atcgtccatc
    23041 caactgtctt cccggctcat gtccggtgtc cgacgcgggc gccgggtcag gcttgcgcgg
    23101 cggcgcgggc cttgttgatg aggctgcgca cggtgtcgct gggctgcgcg ccgttggcga
    23161 tccaggcgtc cacgcgctcc aggttgacct cagctttgtt gtccttccgc gcgggatcat
    23221 aggaccccaa tatctccaga aaacgtccgt cacggcgata acgcttgtcg gcggcgacaa
    23281 ttcgatagat cgggcggcct ttggagcctt cttggcggag acggatggcg actgacatgg
    23341 atgtgatttt tttaaatgct ggatttgaag ggcgcgcaaa ctgcggggcg gcggggggaa
    23401 atcaaatggt ttttattgga tttcccggcc gccatgcggc cggcatttca gctttattcg
    23461 acgacggcag tttcgaccgc agtttcaaca tcgggtgttt ccacaaccgg agcggctttc
    23521 ggagcggact ttttggcggg agcttttttg gcgggagcct ttttggcagg tttggaggcg
    23581 ggagctttgg cagcgggcgc cttgcgcgcc agacccgact tggccagggc ttttttgcgc
    23641 gccaggtaga gtttgcgacg acgttgtttg atgactttgt taagttgctg gcccatgatg
    23701 gtgtgtgcgt gttctgaagg ggatgctcat cctggcaccg tgatccgggg aatcaagatg
    23761 aatatgacag ttgatccgtg atgccattgc aatgcctggc tggatttgaa tgcgggggaa
    23821 agccgttgcc ccggcgagcc gcccgttcat agaacccgga catgaacgcg gaatatctcc
    23881 tcatagatgt cggcaatggc cgcaccaagc tggcactggc ctcgccggaa gctctgctgg
    23941 accggcgtga ctgccctact cccggtctcg atcccggggc agtgcgcgcc attttgaatg
    24001 gatgggattt tcgcagggcc gtgctttgca gcgtggtgcc cgccgcagcg gcggttttcc
    24061 gcggcgtgct gggagaaaaa atgaccgaac tgcgtcatga cacgcccatg ggaatcggcg
    24121 tgcgctaccc atgcccggag agcattggcg ccgaccggct cgcgaatgcc gcggcgctgg
    24181 cgcatttgca cggggctccc ggtgttgtca ttgatttcgg aaccgccgta actttcgaca
    24241 ttctgtccgc cgaccggcat tacatcggag gggtgatcgc tcccggactg cgcctgatga
    24301 ccgactacct gcacgagcgg accgcgctgc tgcccaaagt cgagttgcgc gagccggaat
    24361 gcgccatcgg ccggtccacg gtgcaggcca tcctggctgg cgcggccatc ggctaccgag
    24421 gcatgatcca gggcattctc gatgctttgc gcaaagagct gccacagggg ccggtgcatc
    24481 tcgtcgccac cggcggcgac gcggggtgga tcaccgccgg catggccgag cccatcattg
    24541 tggacccgga tttgaccttg catggattgc gtttggtggg caatctcgcc gccgtttgac
    24601 gccacccgcc acgggcttga tcccgccgca ggcgccaatt ttcaaagccg tccttcccat
    24661 gcccacctac accacctctg tcggaataga tttcggcggt acctctgtca aactcggcgt
    24721 gtgccgcgac ggggaactgc tctgcacgga tgcccccatc cccaccgcga attttcccgg
    24781 tccggcggcg ctcatcggcg agatggcggc gcgtgtggcg aagttgaagg aaaaattccc
    24841 cgccatcgcc gccatcggcg tgggcgtgcc tgggctggtg gacttcgagc acggcttcgt
    24901 ccatgtgctg accaacgtgc ccggctggaa acatgtgccg ctgaaggcca tcctcggcga
    24961 gaagacgggc ctgcctgtca tcgtcgagaa cgacgccaat gccatggcct atgccgagtt
    25021 ccgctatggc gcggggcgcg gcatgaacaa tgtcgtcgcg ctaaccatgg gcacgggcat
    25081 cggcggcggc ctcattttaa acggccagct ctaccgtggc agcggctgcg cggcgggtga
    25141 gatcggccag atgagcatcc actttgacgg agtggccggc cattacggca atctcggcgc
    25201 gctggagaaa tacaccggca accagcagat cgccgagcac gccatgcgcc gctacgccga
    25261 ggcgcacatc cagaaatccg ccgaggattg cacgcccaaa aagatcgccg aagcagccca
    25321 ggccggggac gacatcgcgc gccaagtctg ggatgagatt gccgactggc tcggcacagc
    25381 gctcgccagc atcgcctggc ttttgaatcc ggatgccttt gtcatcggcg gcggagtggc
    25441 ccaggcgggc gacctcattt ttggcccgct gaaccgcaaa gtgcagtcca tgctcagcac
    25501 cgtcgtctgg gagcgcctgc aaatcctccc cgcccgcttc agcaacgagt ccggcatcat
    25561 cggcaatgcg gccctggccg ccgacagtct cggctgaaaa ttcgaagact gacgctcgaa
    25621 atttttaatt tcgaatttcg agcttgagtc ccgtccgcgc tttccagcct ctatctccct
    25681 gcttttttct ttccccatgt ccttagaatc cgaccgcgcc gagctcgccg ccatcctgcg
    25741 ggccaaatct gtcaaaaccg gcaaattcac cctcgcctcc ggcaaggaga gcgatcttta
    25801 tgtggactgc cgggtcacca cactggatgc gcgcggcgct gtgctggtgg gccgcgtttt
    25861 gcatgacttg ctgcggcgcg aggaggcggc gcgcggccat tccatcggct ccctcggcgg
    25921 tctcaccatg ggcgccgacc ccatctcgct cgccgtggca atgacctcca gtctcgcggg
    25981 ggatgccagc cccgtgcagg cattcaccgt gcgcaaggag cccaagggcc acgggcgagg
    26041 gaggcgcatc gagggaaatt tcaccccgca tcagccggtc gtcgtcgtgg acgacgtcat
    26101 caccacgggt gactcgactt tgaaagccat ccaggccatc caggaggagg gtggcagcgt
    26161 ggccttcgcc ctggttttgg tggaccggca ggaggggggc agggcgaaca tcgaaagcaa
    26221 agcctgcccc gtcgtcgccg ccttcacgcg ggaggatctc gtcggctccg cgaatgcctg
    26281 accgtgcctt cccatgcccg ccctctccac ctacaccaag acgctgacga ccgcccaggc
    26341 ggaaaagctg cgcggcattt tgcaggagga gggctggtct ttcgaggaca aagcctggtg
    26401 cctctatgcc gccagccgcg gcaaggtgaa cgtgctggtt tatgaaaaag gccccaaggc
    26461 tgtggtccag ggcaaggaaa ccgagaattt tgtgctcaac accctggagc cgcgcgtgct
    26521 gggagtggcc gagttgggat acgaggaagt ccaccacccg gagttttacc agccgcatct
    26581 tggcgtggat gagagcggga aaggcgattt ttttggcccg ctggtcatcg caggggtgta
    26641 tgcggatgca ggagtggcac gaatgttgcg cgaatgcgga gctgtggaca gcaagcaaat
    26701 cagcagcgac cagaaaattg aaaagctcgc ggaggcggtg agggccattc ccggcctgcg
    26761 ctgggaggtg gtgcagatca accccgaacg ctacaacgcg ctgtatgaaa agttcggcaa
    26821 cttgaaccgc ctgctggcct gggggcacgc caaggtcatc gaactgctgc tggaaaaagt
    26881 gccggactgc ccgcgcgcgg tctccgacca gtttgcgaac cccgccgtgc tccagcgcgc
    26941 cctccaggca aaaggccgcg gcatcgagct tgttcagcga accaaggcgg aaagcgaccc
    27001 agccgtggcc gcggcctcca ttctggcgcg ggagcgtttt gtgagatggc tgcgcgaaac
    27061 cgggcagagc attggcattg ccctgcccaa gggagtctca cccgccgtca aagaaagcgc
    27121 gcgccggatc gttgcctcgc ggggggctga cgctctgact catttcacca agacacattt
    27181 ccgcaccacc gccgaattgc ttgcagcctc ctgacttttc ccccgtattt aagtccaccg
    27241 caccgaccac tccaactctc tcacctcatg gccaatgtca ccaaacgcga acttgtcatc
    27301 gaactgagca acaagctggg ccttcgacag agcgaggtca tgagcatggt tgaggccttc
    27361 atcgaggagg tcagcgccac tcttgagcgg ggagatgagg tcaccctgcg cacgttcggc
    27421 acgttcgaac tgcgcaaggc caaatccaag atcgggcgca atcccaagcg cccgggctcc
    27481 gaggtggtca ttcccgagcg gttcgtggtg cgcttcaagc ccggcatgga gctcaagcag
    27541 cgcgtggccg acctgcccgt gaaggattct aaataacgcc ccgtcccggt cagcggccgt
    27601 gtttgccggc ggtctgtgac gctttggatt tccgcagcag gtattccttc atcgctttcg
    27661 acgccttgga ttcgtggcgc gcggtattcc acatcatggc gaccgtgcgc gccggttttt
    27721 cgccatgaaa ggagcggtac acacggcgct cgctgccgtc aatcctgcgc gccatctcag
    27781 ggacgatgga cactccatga tccagcgcca caagctcctg caccgtcgca agctggctcg
    27841 tgcgctcgac agtcaccggc tggacggtat gctgccggca gaaggatgag atattttccg
    27901 ccaggcagtg cgcctcattg agcatgacga aggggaagtc ctccacggcc tggatggtca
    27961 gaggcgtcac cttcgccaat ggatgcccca ccggggccac caggagcagc tcttcgctga
    28021 acaggggcgc cacttcgagc cccttggcga tgatgggcag cgccaggatc gcaagatcaa
    28081 tctcgccgtg atggcagcgc tggatcaaaa tctccgtcgt gtcctcctgc acgaccaccg
    28141 agatgtccgg gtgcttttgg gcgaagccgc tcagcaatcc gggcaaaaaa tacggcgcta
    28201 tggtcgggat cgcccccagg cggatgcgcc ctcggcggct ggcctctgac aattcggaga
    28261 acgtgtcctc gaccagcttg agaatctctt ttgcccgcgc cagcagcagc tcgcccaaat
    28321 cggtcaggat cacctcgcgc ggatgcctct cgaacagggg ctgtccaagc tggtcctcca
    28381 gcttctggat ggcacggctg agcgcgggct gggaaagatg caggtccgcc gccgcgcggg
    28441 tgaagttccg cgcgcgcgcc agggcgacga attgctcgag ggagtgaagt tcgaattgcg
    28501 tcctcatcgt gggatgagca gggtttagca atgcgcgcac tgcatgacaa gcatagattt
    28561 aatgcattgg aggcattcca ggattgcacg aagcattcac agtccggttc agccaccctg
    28621 acggatttaa cacgagaaac caacctgcca cccaccatga aatcactaga actccgggct
    28681 gtgctcgcca ctctgatgct gacacccgtc gtgtttgcat cagcgcaaga cggcccagcc
    28741 aaacccaact ccgacaaacc catggatcaa ctcagcaaat gccccgtcat gggcgccccc
    28801 gccccggcca gccgccacac cgccgcctca gccatgtcca accgtgactg gtggcccaac
    28861 cagttgaatc tctccatcct tcatcagaac tcgccaaagg ggaaccccct tggcgaggac
    28921 ttcaactatg tggaggagtt caaaaagctc gattacgacg ccctcaaaaa ggacatccag
    28981 gagctgctga ccacctccca ggactggtgg cccgccgact acggccacta cggcccgctc
    29041 atgatccgca tggcctggca cagcgccggc acctaccgcg tcagtgacgg acgtggcggc
    29101 gcgggctacg gcacgcagcg ctttgccccg ctaaacagct ggccggacaa tgccaacctc
    29161 gacaaagccc gccgcctgct ctggcccatc aagcagaagt atggcaacca aatctcctgg
    29221 gccgatttga tgatcctcac cggcaacgtc gcgctcgaat ccatgggctt caaaaccctc
    29281 ggcttcgcgg gcggccgcgc cgatgtctgg gagccgcagg aggacatcta ctgggggcct
    29341 gaaagcgagt ggctcggcga caaacgctac aaaggcgagc gtgatctcga gaaccccctc
    29401 gccgccgtgc agatgggcct catctacgtc aatccggaag g
//
LOCUS       DAOMYJ010000039        28590 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Prosthecobacter sp. isolate AMR_MDS_5629
            NODE_583_length_28590_cov_18.777431, whole genome shotgun sequence.
ACCESSION   DAOMYJ010000039 DAOMYJ010000000
VERSION     DAOMYJ010000039.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867850
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prosthecobacter sp. (wastewater metagenome)
  ORGANISM  Prosthecobacter sp.
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Verrucomicrobiaceae; Prosthecobacter.
REFERENCE   1  (bases 1 to 28590)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 28590)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.30
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:34:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,742
            CDSs (total)                      :: 4,684
            Genes (coding)                    :: 4,676
            CDSs (with protein)               :: 4,676
            Genes (RNA)                       :: 58
            tRNAs                             :: 55
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28590
                     /organism="Prosthecobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_583_length_28590_cov_18.777431"
                     /isolate="AMR_MDS_5629"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1965333"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..381)
                     /locus_tag="PLP58_06225"
     CDS             complement(<1..381)
                     /locus_tag="PLP58_06225"
                     /inference="COORDINATES: protein motif:HMM:NF015534.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Type 1 glutamine amidotransferase-like
                     domain-containing protein"
                     /protein_id="HRJ08145.1"
                     /translation="MKSMLLIFLLPLACSHARAGTLVIVGGGAVGPEILTETLRLAGG
                     AEQARIAILPQASGLPDAGEKSAAVWREAGAADIRVVDLAQSVEARKHLERANLVWMP
                     GGQQSRLVEALRAAEMIPVLRARLA"
     gene            517..1011
                     /locus_tag="PLP58_06230"
     CDS             517..1011
                     /locus_tag="PLP58_06230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ08146.1"
                     /translation="MPPKPLLRIACQMSLALALSALAACSTASIGPGGRISKVKHYHL
                     VPGTMVQTIDTAVAFERDHHLYGAVTRAETRDRLGHYYTIFWKADDRSTPVKVVFDYR
                     QANSGLTLKSMEFPVDEPRRSNATHFQVTGDDYHTGGRVTSWRVRLMRGNEELASQRS
                     YLWN"
     gene            1135..1686
                     /locus_tag="PLP58_06235"
     CDS             1135..1686
                     /locus_tag="PLP58_06235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HRJ08147.1"
                     /translation="MTAAPPAKPATTMLVGWIGKLFWLRVEGKGTFQCSIQLKRAFET
                     VLDRGTKTLVVDLDRCPIMDSTFLGTLTYAASRVRKAGGGSLSVLNANARNLQLLSDL
                     GLDHLMEVDRAGVMWKEEREAARACLAACSESTEATREEQTRHLLQAHKTLSNICESN
                     EGRFRDVIHFLEQELQAPPGGQA"
     gene            1735..3183
                     /locus_tag="PLP58_06240"
     CDS             1735..3183
                     /locus_tag="PLP58_06240"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HRJ08148.1"
                     /translation="MTTYILSAVILLLAGGVIALLAVIRRRDRRITRLQREEQAIVDE
                     EKRMFGYLHELGEAITREDTHATMHRLIVEGAMRVTESQGGALYLLDDAGKVLVPRHY
                     SDHCPPLFDLPERIAAMAKKNAAGLLSFLRLHSLRLDEGLIGRVFKGGGAEMIEDLRK
                     DTRQAGGGSALQGNMGVMAGALSFGPRNLGVLAVASPRGQRVFSSNDFEVFKSLVEQS
                     AFALANVMAHQAASEKRHIEAELRAASEIQRILLPDHDPDLEGYEIAGRNIPAKVLSG
                     DYYDFIPLGGGRHGVVIADVSGKGTAAAIISAMCRSILRGCAPLESSPAAALASVNRQ
                     LAPDIREDMFVSMLYLTFGRNDGRVTLARAGHTLPILWRRETGRIETVKSGGLAVGID
                     KGEVFERVTKDVEFEMRPGDCLLLYTDGINEALDAKGLEYGEQRLHNNLATLAPKGPK
                     AVVDGFIADVEKFLGGKRSLDDITLIALQKKP"
     gene            3246..3863
                     /locus_tag="PLP58_06245"
     CDS             3246..3863
                     /locus_tag="PLP58_06245"
                     /inference="COORDINATES: protein motif:HMM:NF013212.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000774 - adenyl-nucleotide exchange
                     factor activity [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_function: GO:0051087 - chaperone binding [Evidence
                     IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HRJ08149.1"
                     /translation="MTEPSHPSETTPQTQEVPGFEAETPATQPEAAGGPEQAAPADPL
                     TVALQEAAHWKDMAYRNAAELDNFRKRSARDAQETRAYANADLLRGILPILDNFEMGL
                     EAARAESETSMIFMGMSMVQRQIADFLRDMGVQEVEALAKPFDPNLHDAVAQEASDTA
                     AEGTVLRVSRRGYKLKDRLLRAASVVVSSGPPAAPDAIEAGAASA"
     gene            3884..5047
                     /gene="dnaJ"
                     /locus_tag="PLP58_06250"
     CDS             3884..5047
                     /gene="dnaJ"
                     /locus_tag="PLP58_06250"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018291014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HRJ08150.1"
                     /translation="MADKRDYYEVLGTPRDASADDLKKAYRKLAVKYHPDKNPGDKSA
                     EEKFKEVSEAYDVLSDDQKRAAYDRYGHQAFSGGMGGPGGAAGGGFHDPFDIFREVFG
                     GGGGGGIFETFFGGGGGNGGRQRRDGPQRGSDLRYGMEITLEEAAHGCEREIEYERLN
                     SCKSCHGSGSNSGSGRKSCPTCGGVGQVISSRGFFQIQQTCPDCGGSGEVVSDPCRAC
                     SGTGKARERTKVRVKVPAGIEDGSRLRSSGNGDHGSKGGPPGDLYIVVHIRAHDVFER
                     EGDDLHCQMPLSFATAALGGEITVPTLEGKANLKVPSGTQNGTTFRLRGKGVKVLGED
                     RHGDLYVHVQIAVPTKLTSEQRAHLEAFAASFGEAHSSGMEESFFEKAKKFFR"
     gene            5121..5477
                     /locus_tag="PLP58_06255"
     CDS             5121..5477
                     /locus_tag="PLP58_06255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009965735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin"
                     /protein_id="HRJ08151.1"
                     /translation="MSTPKITAYLKTYCGWSEGVRAIFRKYGLDYEEKDIIKNPAFRW
                     EMEQKSGQPLSPCVEINGTMLPDISGEEVEQWMISQGLLTPSEAEPDAPIDRACSDAQ
                     HAAMAAGQAGSIRFVN"
     gene            5581..6903
                     /locus_tag="PLP58_06260"
     CDS             5581..6903
                     /locus_tag="PLP58_06260"
                     /inference="COORDINATES: protein motif:HMM:TIGR00836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008519 - ammonium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0072488 - ammonium transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HRJ08152.1"
                     /translation="MTQPPLPKARRASIGVAAAFAFLAGSVPAHAAESTIESGDTAWM
                     LASTALVLFMMIPGLALFYAGLVRARNVLSIFMQCFVLTCVLSLVWMACGYSLAFTDG
                     SFFIGGLGKAFLSGVTTDTARADCSTIPEVLHCAYQMMFFLITPGLFVGAFAERMKFS
                     AIMIFSIAWSLLVYVPCCYGVWHKAGAFLGLTNVIDLAGGIVVHITAGVAALVLCVVV
                     GPRQDHPTGQIMPHNLPLTMVGAAMLWVGWFGFNAGSQLGAGGTAAMTLLVTHLSSCT
                     AALVWMAVEWKLLGKPSALGVATGCIAGLAAITPASGKVGPLGAVCIGAASALVCWYA
                     CAKLKKKFRYDDSLDVFGVHGVGGFIGTILVAVFGSTSFAGGLGDFEIAPQLATQSLA
                     ALYTIVLSAAGSWLILKVISLFMPLRVTAEQERQGLDLAEHGESAYNS"
     gene            7107..8039
                     /locus_tag="PLP58_06265"
     CDS             7107..8039
                     /locus_tag="PLP58_06265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ08153.1"
                     /translation="MTHKLRSWLIIFGTAAFYGLVCRLLFAWGPLQGWLQIVSASFLY
                     SMPLSLGALVCFLGYKLDQPGRFWALGAPMITMLLLLCGSLLFQLEALFCVLLAAPIV
                     SPFALLGGWLMSVILRRFDGGRLQITVWVLLPYLVSPVEQMWQRPHETRVMRDSIVIH
                     APAGRVWDHIKTVEAIHPGELDWRWIYTLGFPRPIAATLDHEGVGGRRLATFEREVSF
                     YEVVTHWEPHQNCRSPSRPILNSSRAMPLTSTSSWAGASMTSWTALMKSRRGMKIHAC
                     CIFGAPTACPQCSTATPAGGVSGSCTKSKAPSWG"
     gene            complement(8115..8333)
                     /locus_tag="PLP58_06270"
     CDS             complement(8115..8333)
                     /locus_tag="PLP58_06270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ08154.1"
                     /translation="MQIISVLIGIICAILLIPGIIPFLGWLQWATLAGSIVGIIFGAF
                     PKRKIGLIINVAVALVAALRLFLGGGLV"
     gene            8470..9318
                     /locus_tag="PLP58_06275"
     CDS             8470..9318
                     /locus_tag="PLP58_06275"
                     /inference="COORDINATES: protein motif:HMM:NF024141.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRJ08155.1"
                     /translation="MSLFLQQWQGELLKLFARRRTYIGFGAFLLLEIVLLVVFRLQGV
                     ERIFERIISRQGQAFSEYYSALTLAQMILGFSVLILGAIYLALVAGDIVAKEGEDGHY
                     RLLLVRPVSRVRLLFIKYLTCLGYTVVLVQFIAWSAFALGVAIKGWGGGFFAMAPEVG
                     LLVFYEWGDGMAKFASASLFLALAMTVVSSIAFFLSCFPIKPSAATIGALSYVLIDMI
                     LRNTGFMESYDHLLLTKHMAAWARVLAEDVAWPVVIRSFAVIAAVNASLFVLGAAVFE
                     SRDLKS"
     gene            9315..10169
                     /gene="panC"
                     /locus_tag="PLP58_06280"
     CDS             9315..10169
                     /gene="panC"
                     /locus_tag="PLP58_06280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009965018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004592 - pantoate-beta-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HRJ08156.1"
                     /translation="MKCVETVADLRAWRGGAGRVVFVPTMGALHAGHAALVREARRCA
                     GTEARVVASIFVNPLQFGPNEDFDRYPRTLEADLELCAQAGADLVFAPPVREVYHADR
                     SIQILEGSLSRVLCGASRPGHFDGVCTVVAKLFNLVQPDEAVFGKKDWQQLAIIRRLV
                     RDLDFPVRIHGLDTVREGDGLAMSSRNRYLAPEERAQAPALHAALREARDCWLGGSAS
                     ADTLLSVLRGHLARSAPLGQVDYAALVDKHTLQPQAVADRSGLLALAVYFGKARLIDN
                     LELDTTAE"
     gene            10249..10776
                     /locus_tag="PLP58_06285"
     CDS             10249..10776
                     /locus_tag="PLP58_06285"
                     /inference="COORDINATES: protein motif:HMM:NF024805.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HRJ08157.1"
                     /translation="MPPLVGSQLLNARLARGWTLAEAAHETRIPERQLALMEADDYAA
                     FGNLAYARAFLRIYSRHLDVDTSELLAWLALGASRPAPLRLENSNSVWHGAPPPQAKP
                     RRGPLRPLTLALAVLTLILGGGVYSGMHLRMARLLNFPGRPASVLPQPPPEPVPLHAM
                     EKTALPDLRSAGRGS"
     gene            10825..11337
                     /locus_tag="PLP58_06290"
     CDS             10825..11337
                     /locus_tag="PLP58_06290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ08158.1"
                     /translation="MTPNGLPKRWWLPPLLWTAAFLAVLIPALVFSATAREVAAKGVM
                     NILGLLATPFILETVTALAGLALVLAWNQWRIQKDGDGWVYLAKTEPDPASLAAGADK
                     AEHRLAAVVLAEKPDSALDFEARLAVAEGYLELGLGREALEHLDMLAPDEQAHERAAA
                     LRVKAASLAA"
     gene            11356..12915
                     /locus_tag="PLP58_06295"
     CDS             11356..12915
                     /locus_tag="PLP58_06295"
                     /inference="COORDINATES: protein motif:HMM:NF018471.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipase maturation factor family protein"
                     /protein_id="HRJ08159.1"
                     /translation="MAPDSQPSGAGNWRPGRYRVTRWVFPRLLAVVYLCAFASWAVQW
                     EGLLGEEGILPVPQIVENIHAYEAREQTTLWLEYPSVLRWRSDDAFAQMLCWGCCGLA
                     LLVMAGLWQGPLLLVLWAAYLSLAAVGEIFMGYQWDALLLEAGLLGVFVAPWSLRPGG
                     LRAAPGDPPWMSVVLLHWLVFRLMFLSGFVKLAGGDSTWWECTALLHHYETQPLPNGL
                     SWFAHHLPRWLHTASCAAMHVIETGLPFAIVLGRWGRRAAAGGFVLLMALIFATGSYN
                     FFNLLTAALALCLLDDTSWPRRLRAWLRLDDGAPPPPRWHWRQWPALAFAPLCLLLTL
                     AAADLFMAGRVSWHRGFMPEWARQFYARTQSLRSFNAYGLFQAMTTERPEIIVEVSDD
                     GALWQPLEFKWKPGRQDRAPGFAAPHQPRLDWQMWFAALAPGFDPRRDSQPGSPLYWF
                     GRFAACLLEKKPAVWALVEPPPLPVEDIRHVRAKLYRYRFSSPAHRRQTGEWWTREYL
                     GMWSPVFSVKR"
     gene            13010..13972
                     /locus_tag="PLP58_06300"
     CDS             13010..13972
                     /locus_tag="PLP58_06300"
                     /inference="COORDINATES: protein motif:HMM:NF012318.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HRJ08160.1"
                     /translation="MRPIFLTALLFPALLAADLPDPPVTLERLQAIQDAVRDLFPAAR
                     PAVVAILTGDGTASGVIIDKSGLVLTAAHVAETPGRELRVVLEDGRRVTGTTLGLDKT
                     TDAALLQLNDTKRDWPHAAVSRRVIEAQPGEWCFALGHPGGFDKARGVVLRVGRIIRQ
                     TANSLQTDCVLMGGDSGGPLFNLKGEVIGIHSQIWEKRDENMHVSMAPFLRAWDEMKS
                     SLVIRTWGTGSGGYLGLAADVNPDTELLEVLEVLAGSPSEKAGVLPGESILALDGEPL
                     GGLSHLSNAVKLRAAGDQVRLRLKKGGQEREVSVTLGHRPKEEG"
     gene            13979..15001
                     /locus_tag="PLP58_06305"
     CDS             13979..15001
                     /locus_tag="PLP58_06305"
                     /inference="COORDINATES: protein motif:HMM:NF024578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HRJ08161.1"
                     /translation="MRPALCLILLAAGASAEMRPSLQPEDRKNGSRTLLALDPVHRQA
                     IKATARLVDTGGRAAALATWVGEDGYLITKASEAPELEKLQVRLADGSLAALREIRRD
                     TRHDLVLAQAIGVTGVTAIPFTADAKAPAFGQWITAPAADGQLRLGVISASSRQIPGL
                     GAAIGVRMDEKPAPGGKGVRVLAVAEDSPARAAGLRDGDILLSLDDDTLSQFSQVSQL
                     ISKRQPGEIVAVKYLRDGRERACKVRLASRSKVLNNWEGEDFANGGISLRTDSFEKIL
                     QHDLPLGPADMGGPLLDLSGRPLGINIARVDRITTFALPAAVFWPLVKGWIEADRHPP
                     KALPAR"
     gene            15159..15608
                     /locus_tag="PLP58_06310"
     CDS             15159..15608
                     /locus_tag="PLP58_06310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ08162.1"
                     /translation="MKFLCAASLMALASAAAVRADGVPFDRVTGRVTVPHTVLHMSAE
                     QRAQVSQTRRLTLTPRQHERLARLCPVFPNEIGEVLSYRYGDCTCCVGHPYALLLPGG
                     ASVAVPHSEAEDVSRYGVRERPSFAATTTAGQRSRWFRFWRAVFRRD"
     gene            15730..16113
                     /locus_tag="PLP58_06315"
     CDS             15730..16113
                     /locus_tag="PLP58_06315"
                     /inference="COORDINATES: protein motif:HMM:NF025484.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4288 domain-containing protein"
                     /protein_id="HRJ08163.1"
                     /translation="MKKPATSRTGWWIAGLLEKHSNTDRPTYWNNYRLIKAGDWRTAF
                     RKAAELGAANARVGNKAFSGHQEFIGVTDLLPIYDEFEDGAELLWQELEASQDDDGIP
                     LRVFTVTDLESQYELPGDDGVPANA"
     gene            complement(16153..17106)
                     /locus_tag="PLP58_06320"
     CDS             complement(16153..17106)
                     /locus_tag="PLP58_06320"
                     /inference="COORDINATES: protein motif:HMM:TIGR02136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein"
                     /protein_id="HRJ08164.1"
                     /translation="MTTAAILCLPAAGSGALRAVEPDAALPEYHPSARVSGSIISVGS
                     DTLNNLMTLWAEGFKAWHPEVILQVEGKGSATAPPALIAGTCQLGPMSRPMKQEELDA
                     FEKKFGHPPLAVRVAVDALAVFAHKDNPARGLTLAQLDAAFSSTRRMGAPADITEWAA
                     LGVPGWAGRPVSLYGRNSASGTYGFFKEHVLGRGDFKSSVKEQPGSSSVVQSVSADTH
                     AIGYTGIGYLTSGVRVLPVGQEEGGFALPTYENCINGSYPLARFLLIYLNKKPGRPLD
                     AATAEFVRYVHSREGQRVVVKDGYYPLPAQVIEETRALLAR"
     gene            17261..18040
                     /gene="pstB"
                     /locus_tag="PLP58_06325"
     CDS             17261..18040
                     /gene="pstB"
                     /locus_tag="PLP58_06325"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004810563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HRJ08165.1"
                     /translation="MEATAPSTPLSIRVRDLDFFYGPQQALDGVSLDMPRQRATALIG
                     PSGCGKSTLLRCFNRMNDRIPDAVVRRGGIEVEGRDVLAPGLDVVSLRREVGMVFQKS
                     NPFPLSVFENVAYGLRVKGESRADYLEHVVEESLRAAALWDEVKDQLHSDAGALSGGQ
                     QQRLCIARAIALRPRVLLMDEPCSALDPVATARVEELIHGLKRRFTVVIVTHNMQQAM
                     RTSDFTAFFYMGRLIEHAPTQAFFENPREKMSQDYVTGRFG"
     gene            complement(18045..19418)
                     /locus_tag="PLP58_06330"
     CDS             complement(18045..19418)
                     /locus_tag="PLP58_06330"
                     /inference="COORDINATES: protein motif:HMM:NF016612.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="neutral/alkaline non-lysosomal ceramidase
                     N-terminal domain-containing protein"
                     /protein_id="HRJ08166.1"
                     /translation="MKFLAVLACAGCILSIPAVLKAKGWQAGAAKTDVTPDFPVRLSG
                     YGSRNSPHEGVSLPIHAKALALRWEADEPVVMLTVDNCGIPAAMRAEVLANVRNAGWK
                     LADERFSLHSSHTHCAPMVNGVLGFLFGADLPPDERKAVDRYTAFLTARMTEVALSAL
                     NDLKPAQVDRGAGKAGFAMNRRLKTETGYANSPNFEGVRDHDLPVLRVRDMEGKLRAL
                     FTSYACHCTTLAINHIHPDWAGTAQAELELRFPGVVALTAIGCGADQNPFPRREMEHA
                     LKHGVTLAKGVIHAINNPMKPLPGPVICRSKDLQLPFDTLPGRAEWEMRTQDKNKWIA
                     HHARHFLGMMERGEKIPDTLPYNVQVWSFGGGLLMIHLPGEVVVDYGLRFKKSFGRQR
                     TWVNAYTNDVPCYIPSQRVWEEGGYEAAGAMTYYGRPTRFASGVEEIIAGAVNELTPQ
                     VFKTPGK"
     gene            19553..20896
                     /locus_tag="PLP58_06335"
     CDS             19553..20896
                     /locus_tag="PLP58_06335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009960670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1552 domain-containing protein"
                     /protein_id="HRJ08167.1"
                     /translation="MMKNSPSYSRRQFLRGAGVLLGLPWFESVSSFGAPVVKGTPQAP
                     RRFAACFFGNGVNPHHWGAGNGAGGLEFLQTLKPLEPVKDQVIVLKGLWNPTTVAGPG
                     GHYPKMNILSGLKVKQTTTDVEVGVTMDQIIAAKVGRETPVASLAIGTEGPKYSTDSG
                     YTSLYSAYLSWSSPTTPAPKEIYPQQAFDQLFDDGSKRARDKSVLDLVLSDARSLRGK
                     LSRRDGQKLDEYLASVRDLEQRIERAEKLNAAEADTRGWQPSVRTPWLARPASGIPAR
                     QEEHVRLMLDIMVLAFQMDRTRVITNMLTNDLSNMNFGFIGVKGGQHEFSHHANDSER
                     LDGYQKSNEFMVQAWSEALQKMHATREGERTLLENSMILLTSSLYDGNAHDSTQLPLV
                     LAGGGGGTLRGGRCVEFDKKDENRKLCRLHLALMERMGVKTDRFGDADNSLGELA"
     gene            complement(21230..22498)
                     /locus_tag="PLP58_06340"
     CDS             complement(21230..22498)
                     /locus_tag="PLP58_06340"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase"
                     /protein_id="HRJ08168.1"
                     /translation="MKPILACLMMILGLSVHAAGPNVILINCDDLGYGDLGCYGAKDI
                     RTPHLDRMAAEGTRFTDFSVTSALCTPSRAALMTGKYPGRVGLATGVLRPDAKNGLAG
                     SETTLAEVFKAAGHATGCIGKWHLGFVKGMRPVDQGFDSYYGVLHNLDHWETVVFEKE
                     GGMPVLRGDAVEKRPAVPAEMTALYTAEALKFIVQHRQRPFFLYLGHAMPHLPFDASP
                     KFKGKSQRGLYGDVVEELDDSTGQILDKLRALGLAEQTLVIFTSDNGPERKTPGTAAP
                     LTGTKHTVYEGGLRVPCIAWWPGKVPANRVCGEMLTTLDLLPSFARLCETQPSANLDG
                     IDQTDLFLNANAKSRRTTLYSLYGYKERRLESFREGRWKLHLTPKTMLFDLQTDLAEQ
                     NDVSADHADIVGKLTRLAIGLRGAELTPPN"
     gene            22560..23492
                     /locus_tag="PLP58_06345"
     CDS             22560..23492
                     /locus_tag="PLP58_06345"
                     /inference="COORDINATES: protein motif:HMM:NF012742.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HRJ08169.1"
                     /translation="MKLIPTLLLAASVSGIDSCSPQPSSPPSDKPKVALVMKSLANEF
                     FQTMAEGAKKHQAAHAADYDLVVNGIKNETDLAEQVGLVEQMVAQGVQAIVIAPADSK
                     ALVTVLKRAKDAGVLVINIDNKLDADTLKQAGLQIPFVGPDNRAGAKAVGEVLAKNLK
                     AGDEVAIIEGVTTAFNGQQRKAGFEDAMNAAGMKIVSSQSGQWEMEKANGVAAGIIAA
                     NPNVKALLCANDNMALGAAAAVQAAGKAGQIQIVGFDNIAALKPLLADGRVLATADQH
                     GDQLAVFGIEAALKILKDKSTPADQQTPVDVVTK"
     gene            23504..23743
                     /locus_tag="PLP58_06350"
     CDS             23504..23743
                     /locus_tag="PLP58_06350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ08170.1"
                     /translation="MEKVFQRICKGYRERPWASLFYSFLIYLFIAIALVLASPSLSDS
                     TPGRLTDEERGDGIVLTVIVLFLAGLGGGWLAAKK"
     gene            23759..24682
                     /locus_tag="PLP58_06355"
     CDS             23759..24682
                     /locus_tag="PLP58_06355"
                     /inference="COORDINATES: protein motif:HMM:NF014684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRJ08171.1"
                     /translation="MRRFADYLILLAVLAAMILLFGMLRDSFLSAATLGSVANRIPAL
                     ALVATGMTLVMVTGGIDLSVGSVLGLCGAVVGVMMADWQSGLLLALLGSILVGGAIGG
                     TTGAVSVKLRLPSFIVSLGMLEMARGLAFLATNSQTKYIGASIESLGAPFGGLVISPA
                     FVISVLVVVAGQVMLSQMPMGRHWIAIGANREAALVSGVPVDRPRILAHALLGALTGL
                     AAVFNCSRLGSADPNAGVGFELSAIAAVVVGGTSLMGGRGSVVKSFMGVLIIATLEAG
                     LVQIGATEPVKRVVTGAVIIAAVLADTWRRK"
     gene            24686..25669
                     /locus_tag="PLP58_06360"
     CDS             24686..25669
                     /locus_tag="PLP58_06360"
                     /inference="COORDINATES: protein motif:HMM:NF013335.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside hydrolase"
                     /protein_id="HRJ08172.1"
                     /translation="MKRLILFLCAFSASLFAEKPKIIFDTDITGDVDDVLALAMCHTL
                     ADRGACEFLGVTVSKNNPLTASFVDAQNTFHGRPDLPVGVTRDDKAQHRESKYLKLAD
                     SADYPHDLKRNEDAKDAVELIRELLTAQPDHSVTIISVGIASNMANLLKSPGGVALVK
                     QKVKALSIMAGAFAYCNGTNYHLEANVINGIGYMQTVADQWPDEVPIVWSGYEIGEAL
                     PFPRVVIQRDLGYVPHHIVKEAYLLHSGPEHDRPCWDESSVLWAVYPERSFFGLSEPG
                     RVKVLNDGFTRFMPQGKAQSSKRDRFLTMSSVQQSRALEAIVHLTIQPPKK"
     gene            25666..26625
                     /locus_tag="PLP58_06365"
     CDS             25666..26625
                     /locus_tag="PLP58_06365"
                     /inference="COORDINATES: protein motif:HMM:NF013335.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside hydrolase"
                     /protein_id="HRJ08173.1"
                     /translation="MKHLLFSLLFCGLAQAAPVKLIFDTDMGNDVDDLMALCMIHNLQ
                     KRGACELLAVTVTKDHPQAAAFVDAVNTLYGYPDTPIGVVRNGAAKEAGKFNLLADKY
                     PHDLKSGAEAPEAGGLIKDILAKQPDGSVVIAQVGFFTNLARLMDDAEAKALIAKKVK
                     VLSLMAGAFQTVNFNTRHLEYNVKLDIPAAQKLAKEWPTPMVWSGFEIGVAAAFPHVV
                     IEQDLDYMPQHPLKEAYYLYNPPPHDRPTWDPTSVLYAIYPDRGYFDLSPPGNVIVED
                     DSATLFRPTKDGKGKHRFLIMSPEQAARAREAIVQLSVEPPAA"
     gene            26991..>28590
                     /locus_tag="PLP58_06370"
     CDS             26991..>28590
                     /locus_tag="PLP58_06370"
                     /inference="COORDINATES: protein motif:HMM:NF016858.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HRJ08174.1"
                     /translation="MLERIIYFENYVVVDAGDTSLSKMQTLTEDEYQDAREQFGERFV
                     ARMGAEAIKMLLEEIDIEQLSAELHTQLSTTTSMQARAKAIKRLKVVEAIRKSGNSAA
                     WMVLDVVPVIPPDLRPLVPLEGGRYATSDLNDLYRRVINRNNRLKRLIELRAPEVILR
                     NEKRMLQEAVDALFDNGRHGRTVLGAGNRPLKSLSDMLKGKQGRFRQNLLGKRVDYSG
                     RSVIVVGPELKLHQCGLPKKMALELFEPFIIRELKERSIATTIKAAKKIIAQGREEVW
                     DILEEVIKDHPVLLNRAPTLHRLGIQAFEPVLIEGKAIRIHPLVCAAFNADFDGDQMA
                     VHVPLMPGAQLEARLLMLSSTNIFSPSNGRPIVTPSQDIVLGISYMTRLRKGAKGGWK
                     PEWMKDGKIIEPGRIFRDTASVVLAYEMDEVALHAHVKVWTDGEIVDTTVGRVLLKEA
                     LPPEIALKDVNREQDKGSIGDVIANAYITHGHSKTVELLDMMKRVGFKYATLGGISVG
                     VVDMLVPVEKKQLIDSAKQEVARID"
BASE COUNT         4957 a         9373 c         9108 g         5152 t
ORIGIN      
        1 ggccaggcgc gctcgcagca cggggatcat ctccgccgcg cgcagcgcct ccacgagccg
       61 gctctgctgg cctcccggca tccagaccag gttcgcgcgc tccagatgct tccgcgcctc
      121 cacggattgc gcgaggtcca ccaccctgat gtccgccgct cccgcctcgc gccagacggc
      181 ggcggatttt tctcccgcgt ccggcagccc ggaggcttgc gggaggatgg cgatgcgcgc
      241 ctgctccgcg ccgccggcca gccgcagggt ctccgtcaaa atctccggtc cgaccgcgcc
      301 gccgcccacg atgacgaggg tccccgcgcg ggcatgcgag caggccagcg gcaggaggaa
      361 aatcagcagc atggatttca tgagggcggt ttatcacaaa caccgacggg cgcgcaaggc
      421 ggcaatattt ttgccggcgc gcgccgcttc atttttaaac tgcgcatcgg cggcgttgac
      481 atcgcctgtt ccgactgaat aatgcgcccc cagtccatgc cccccaagcc gcttctccga
      541 atcgcatgcc agatgtcgct ggcgctggcc ctttccgcac tggccgcatg ctccacggcg
      601 agcatcggcc ccggaggcag gatttcgaaa gtgaagcact atcatctggt tcctggaacg
      661 atggtgcaga ccattgacac cgccgtggct tttgagcgcg accaccatct ttacggggcg
      721 gtgacgaggg ccgagacccg tgaccgcctc gggcattatt acaccatctt ttggaaagcc
      781 gacgaccgct ccacgcctgt gaaggtggtc tttgattacc ggcaggcgaa cagcggcctc
      841 acgctcaagt ccatggagtt tcccgtggac gagccgcgcc ggtcgaatgc cacccacttt
      901 caagtgaccg gcgacgacta tcacaccggc gggcgcgtca cgagttggag ggtgcgcctg
      961 atgcgcggca atgaggaact ggcctcgcag cggtcttatc tctggaactg aacatgcggc
     1021 gcgcggcaca aatcccccaa gtccatgatc aacgccccct ccgacagcgg cggaagcgat
     1081 gcgtatccca tgccggccgc gcggcgtaac caacagtcaa cacccccacc gtccgtgacc
     1141 gcagcgcccc cagcaaaacc cgcgacaacg atgctcgttg gatggatcgg caagctgttc
     1201 tggctgcgcg tggaagggaa ggggactttc cagtgctcca tccagctcaa gcgcgccttt
     1261 gagaccgtgc tcgacagggg gacaaagacg ctcgtggtgg acctggaccg ctgtcccatc
     1321 atggactcca cctttctcgg cacgctgact tacgcagcca gccgcgtccg caaggccggc
     1381 ggcggctccc tcagcgtgct caacgccaac gcgcggaatc tgcagctgct cagcgatctc
     1441 ggcctggacc acctaatgga ggtggaccgg gcgggtgtga tgtggaagga ggagcgcgag
     1501 gccgcccgcg cctgcctggc cgcatgctcc gaaagcaccg aggccacccg cgaggagcag
     1561 acgcgccacc tcctccaggc gcacaagacc ctctcaaaca tctgcgagtc caacgaggga
     1621 cgcttccgcg atgtcattca ctttttggaa caggaactgc aggccccgcc cggcgggcag
     1681 gcatgacgga cggaggacgc ggcctggcat ggcgcgcgcc gtgacgaagc atccatgacg
     1741 acttacattt tatcggcggt catcctcctg ctggcgggag gtgtcatcgc cctgctggcg
     1801 gtcatccgcc gcagagaccg gcgcatcacc cgccttcagc gcgaggagca ggccatcgtg
     1861 gacgaggaga aaaggatgtt cggctacctg cacgagctgg gcgaggccat cacccgcgag
     1921 gacacccacg ccaccatgca ccgcctcatc gtcgagggcg ccatgcgtgt cacagagagc
     1981 cagggcgggg cgctctacct gctcgatgac gccggcaagg tgctcgtgcc caggcattac
     2041 tcggaccact gcccgccgct cttcgacctg ccggagcgca tcgccgccat ggcgaaaaag
     2101 aacgccgccg ggctccttag tttcctgcgc ctgcacagcc tgcgcctgga cgagggcctc
     2161 atcggcaggg tcttcaaagg cggcggggcc gaaatgatcg aggatttgcg caaggacacg
     2221 cggcaggccg gcggcggctc cgccttgcag ggcaacatgg gcgtcatggc gggggcgctc
     2281 agtttcggcc cgcgcaacct tggtgtgctg gctgtggcca gcccgcgcgg ccagcgcgtg
     2341 ttcagcagca acgacttcga ggtcttcaaa tcgctcgtcg agcagtccgc ctttgccctg
     2401 gccaatgtca tggcccacca ggcggcctcg gaaaaaaggc atatcgaggc cgagctgcgc
     2461 gccgcgagtg aaatccagcg catcctgctg cccgaccatg atcccgatct tgaaggttac
     2521 gagatcgccg gccgcaacat ccccgccaag gttctcagcg gggactatta cgacttcatc
     2581 cccctgggcg gcggccgcca cggcgtggtc atcgccgatg tctccggcaa gggcaccgct
     2641 gccgccatca tcagcgccat gtgccgcagc atcctgcgcg gctgcgcgcc gctggaaagc
     2701 tcccccgccg ccgctctcgc ctcagtcaac cgccagctcg cccccgacat tcgcgaggac
     2761 atgtttgtca gcatgctcta cctgaccttc ggccggaatg acggcagggt cactctcgcc
     2821 cgcgccgggc acaccctgcc catcctctgg cggcgcgaga ccggacgcat cgagacggtc
     2881 aaaagcggcg gactcgccgt cggcattgac aagggggagg tattcgagcg cgtcaccaag
     2941 gacgtcgaat tcgagatgcg gcccggcgac tgcctgctgc tctacacaga cggcatcaac
     3001 gaagccctcg acgccaaagg cctagaatac ggcgagcagc gcctgcacaa caacctcgcc
     3061 accctcgcgc ccaaagggcc gaaagccgtg gtggacggat tcatcgccga tgtcgagaag
     3121 ttcctcggcg gcaaacgctc cctggatgac atcaccttga ttgcgctcca gaaaaagcca
     3181 tagtcgcgcc cccctgggag atcgtttcct ggatttccca acgcaaaaac ttcccgcgac
     3241 accgcatgac cgaaccgagc catccatcag aaaccacccc gcagacgcag gaggtgcctg
     3301 gcttcgaagc cgaaacccca gccacccagc cggaggcagc cggcggtccg gagcaagccg
     3361 cccccgccga cccgctcacc gtggcacttc aagaagccgc gcactggaaa gacatggcct
     3421 accgcaacgc cgccgagctt gacaacttcc gcaaacgctc cgcccgcgat gcccaggaaa
     3481 cccgcgccta tgccaacgcc gacctgctgc gcggcatcct gcccattctc gacaacttcg
     3541 aaatgggcct cgaagccgcg cgcgccgagt cagaaacgtc catgatcttc atgggcatga
     3601 gcatggtgca gcgccagatc gccgatttcc tccgcgacat gggagtgcag gaagtcgagg
     3661 ccctcgccaa gcccttcgat cccaacctcc acgacgccgt cgcccaggag gccagtgaca
     3721 cggccgccga aggcaccgtc ctccgtgtca gccgccgcgg ctacaagctc aaagaccgcc
     3781 tcctgcgcgc agccagcgtc gttgtttcat cgggaccgcc cgccgccccc gacgcaatcg
     3841 aagccggtgc ggcatccgcc tgacccccta tttttccacc cgaatggcag acaaacgcga
     3901 ctattacgaa gttctcggca caccccgcga cgcctcagcc gacgacctca aaaaggccta
     3961 ccgcaagctc gccgtcaaat accatcccga caagaatccc ggtgacaaaa gcgccgagga
     4021 aaagttcaag gaggtcagcg aggcctacga tgtgctcagc gacgaccaga aacgcgccgc
     4081 ctatgaccgc tacggccacc aggcattctc cggcggcatg ggcggccctg gcggcgcggc
     4141 aggcggcggc tttcacgatc ccttcgacat attccgcgaa gtcttcggcg gcggcggtgg
     4201 aggcggcatt tttgaaacct tctttggcgg cggcggaggc aatggcggac gccagcgccg
     4261 ggacggaccc cagcgcggca gcgacctccg ttatggcatg gagatcaccc ttgaggaggc
     4321 cgcccacggc tgcgagcgcg agatcgaata cgagcgcctc aactcctgca aatcctgcca
     4381 cggcagcggc tcaaacagcg gctccgggcg caaatcctgc cccacctgcg gcggcgtcgg
     4441 tcaggtcatc tcctcgcgcg ggttcttcca gattcagcag acctgccccg actgcggcgg
     4501 cagtggcgag gtcgtcagcg acccctgccg cgcctgctcg gggaccggca aagccaggga
     4561 gcgcaccaag gtccgtgtca aagttcccgc cggcatcgag gacggatcgc gcctgcgttc
     4621 cagcggcaac ggcgatcacg gcagcaaggg cggcccgcct ggcgatctct acatcgtcgt
     4681 tcacatccgc gcccacgatg tgttcgaacg cgagggcgac gacctccact gccagatgcc
     4741 actctccttt gccaccgccg cgctcggagg cgaaatcacc gtgcccaccc ttgagggcaa
     4801 ggccaacttg aaagtgccct ccggcaccca gaacggcacc accttccgcc tgcgcggcaa
     4861 aggcgtcaaa gtgctcggcg aggaccgcca cggcgacctc tacgtccacg tccagatcgc
     4921 cgttcccacc aagctcacct ccgagcagcg cgcgcatctt gaggcgttcg cagcctcctt
     4981 cggcgaggcg cactcctccg gcatggagga gagcttcttt gagaaggcga aaaaattctt
     5041 ccgctgatcc cgctccgttc cgagctggac agggccgcca gacgtaacac actccgcaca
     5101 cacaacccgc acacaccatc atgagcacac cgaaaatcac cgcctacttg aaaacctact
     5161 gcggctggag cgagggcgtt cgcgccatct tccgcaaata cggcctcgac tacgaggaaa
     5221 aggacatcat caagaatccg gccttccgct gggagatgga gcaaaagagc ggccagcccc
     5281 tcagcccctg cgtcgaaatc aacggcacca tgctgcccga catcagcggc gaggaagtgg
     5341 agcagtggat gatctcccag gggcttctca cccccagcga agccgaaccc gacgccccga
     5401 ttgaccgcgc ctgcagcgac gcccagcacg ccgccatggc cgcaggccag gccgggagca
     5461 tccgttttgt gaactgagcc ggtttctggg gacgcggcaa aatccccggt tttggcaaaa
     5521 gcttgctaat tttttcccgg aatggtgcta ttgggtgagt cccaacctca cccaacaacc
     5581 atgacccaac caccgctccc gaaagcgcgg cgcgccagca ttggcgtggc cgctgcattt
     5641 gcgtttctcg ccggctccgt ccccgcccat gccgccgagt ccaccatcga atccggggac
     5701 accgcctgga tgctggcatc caccgcgctt gtcctgttca tgatgatccc ggggctggcg
     5761 ctgttttacg ccggcctggt gcgcgcgcgc aacgtgctct ccatcttcat gcagtgcttc
     5821 gtgctgacct gcgtgctcag cctggtgtgg atggcgtgcg gttactcgct ggccttcacg
     5881 gacggcagct ttttcatcgg cggcctaggc aaggcattcc tttccggagt gaccacggac
     5941 acggcccggg ccgactgctc caccatcccg gaggtgctgc actgcgccta ccagatgatg
     6001 tttttcctga tcacgccggg gttgttcgtg ggcgccttcg ccgagaggat gaaattcagc
     6061 gccatcatga tctttagcat cgcctggagc ctgctggtgt atgtgccctg ctgctacggc
     6121 gtgtggcaca aggcgggcgc cttcctgggg ctgacgaatg tcattgacct ggccggcggc
     6181 attgtcgtcc acatcacggc aggcgtggcg gctttggtgc tgtgcgtcgt ggtcgggccg
     6241 cgccaggacc acccgaccgg gcagatcatg ccgcacaatc tgccgctgac catggtcggc
     6301 gcggcgatgc tttgggtggg ctggttcggc ttcaacgcgg gcagccagct cggcgccggc
     6361 ggcacggcgg cgatgacgct gctggtgacg catctctcct cctgcacggc ggcgctggtg
     6421 tggatggcgg tggagtggaa gctgcttggc aaaccgagcg ccctgggcgt ggccaccggc
     6481 tgcatcgcgg gtctggcggc gattaccccg gcatcgggga aggtggggcc gctgggcgcg
     6541 gtctgcatcg gcgcggcgtc agccctggtg tgctggtatg cctgcgccaa actcaaaaag
     6601 aagttccgct atgatgactc gctggatgtc ttcggcgtcc acggagtggg cggttttatc
     6661 ggcacgatcc tggtcgcggt cttcggctcc acctcctttg ccggcggcct gggtgatttc
     6721 gagatcgcac cccagctcgc cacccagagc ctcgccgcgc tttacaccat cgtcctctcc
     6781 gccgcgggaa gctggctcat tttgaaagtc atctccctct tcatgccgct gcgcgtcacc
     6841 gccgagcagg agcgccaggg cctcgacctg gccgagcacg gcgagtccgc ctacaactcc
     6901 tgataacggc cccaagaaaa acccggcgcg gataaacgcg ccgggttttt tgattttttg
     6961 aggtgacgcg gagaactttc tcactctgca ggccgtggcg cacagcatcc gtttgatgaa
     7021 caggtttcgc tcgtgttgaa agaagtgaaa atacccggct ggacatatgc cttgcttaca
     7081 tgcggcgcgg ggattgaatg ccgcgcatga cccacaaact gcgctcctgg ctcatcatct
     7141 ttggcacggc cgcattttat ggcctcgtgt gcagattact gttcgcgtgg ggaccgctgc
     7201 aggggtggct tcagattgtc tcagcctcct tcctctacag catgccgttg agcctgggag
     7261 cgctggtttg ctttctgggt tacaaactgg atcagcccgg ccgtttttgg gcgttggggg
     7321 cgccgatgat cacgatgctg ctgttattgt gcggaagttt gttattccaa cttgaggccc
     7381 tgttttgtgt tctgctggcg gctccgattg tctcgccatt cgcgcttctt ggcggctggc
     7441 tcatgagtgt catactgcgc cgttttgatg gcggcaggct tcagatcacc gtctgggtgc
     7501 tgctgcccta tctggtctcg cctgtcgagc agatgtggca aaggccgcac gagacacggg
     7561 tcatgcgcga ctccatcgtc attcatgctc ccgccggtcg cgtgtgggac cacatcaaga
     7621 ccgtggaggc cattcatccc ggggaactgg actggcggtg gatttacaca ctgggttttc
     7681 cccgccccat cgcggccacg ctggatcacg agggtgtggg aggcaggcgt ctcgccactt
     7741 ttgagcgcga agtctctttt tacgaggtgg tcacgcattg ggagccgcat caaaattgtc
     7801 gttcaccatc aaggccgatc ctgaattcat cccgcgcaat gcctttgacg agcacatcat
     7861 cgtgggcggg cgcttctatg acgtcctgga cggcacttat gaaatcgagg cgcgggatga
     7921 aaattcatgc gtgctgcatc tttggagcac ccaccgcctg tccacaatgt tcaacagcta
     7981 cgccggctgg tggagtgagc ggatcatgca ccaaatccaa ggctccatcc tgggggtaat
     8041 ccgcgcacgc tgtgaattcc ggaggtgagt gtccgccggc ccgctttact gactgccgaa
     8101 atagcgaaag attgtcaaac caagccgccg ccgaggaaca ggcggagggc ggcgacgagg
     8161 gccacggcga cgttgatgat gaggccgatt ttgcgtttcg ggaaggcgcc gaagatgatg
     8221 ccgacgatgc tgcccgcgag tgtggcccac tgcagccagc ccagaaacgg gatgatgccg
     8281 gggatgagca ggatggcgca gatgatgccg atgaggacgg agatgatttg catagcgccg
     8341 ccatgacagc ctccccgggt ctttggcgca atgggaattt tccgccgatt ttgcagcgcc
     8401 gcctcccggc ggcgcgcggc cacattcctg attgcgcggc gcgttttccc ggacttcgaa
     8461 tccgcccgca tgtccctctt tctccaacaa tggcagggcg agcttttgaa gctcttcgcc
     8521 agacgccgca cctacatcgg gtttggcgcg tttttgcttc tggagatcgt gctgctcgtc
     8581 gtcttccggc tgcaaggagt ggagcgcatt ttcgagcgca tcatttcccg ccaggggcag
     8641 gctttttcgg aatactactc ggcgctgacg ctggcgcaga tgatcctcgg cttctccgtg
     8701 ctcatcctcg gcgccatcta tctggccctg gtggcgggcg acatcgtggc gaaggagggc
     8761 gaggacggcc actaccgcct gctgctggtg cggccggtga gccgggtgcg gctgcttttc
     8821 atcaaatacc tcacctgtct gggctacacg gtggttctcg tgcagttcat cgcgtggtcg
     8881 gccttcgcgc tcggcgtggc catcaagggc tggggcggcg gtttctttgc gatggcgcct
     8941 gaggtggggc tgctggtgtt ttacgagtgg ggcgacggca tggcgaagtt tgcctcggcg
     9001 tcgctgttcc tggcgctggc gatgacggtg gtgagcagca tcgccttttt tctctcctgc
     9061 ttccccatca agccgtcggc ggccaccatc ggcgcgctgt cctatgtgtt gattgacatg
     9121 atcctgcgca acacgggctt catggaaagc tacgaccacc ttttgctgac gaagcacatg
     9181 gccgcctggg cgcgggtgtt ggccgaggat gtggcatggc cggtggtcat ccgcagcttc
     9241 gcggtgatcg cggcggtgaa tgccagcttg tttgtgctgg gggcggcggt ctttgaatcc
     9301 agggatctga aatcatgaaa tgcgtggaga cagtggcgga cttgcgggcc tggcgtggcg
     9361 gggctggaag ggtcgtcttt gtgcccacca tgggcgcgct gcacgcgggc catgccgcgc
     9421 tcgtgcgcga ggcgcgccgg tgcgcgggca cggaggcgcg cgtggtggcg agcatttttg
     9481 tgaacccgct gcagttcggc ccgaacgagg actttgaccg ctacccgcgc acgctggagg
     9541 cggacctgga gctgtgcgcg caggccgggg ccgacctcgt ctttgcccct ccggtgcggg
     9601 aggtctatca cgccgaccgc agcatccaga ttctcgaagg cagcctctcc cgtgtgcttt
     9661 gcggcgccag ccggcccggc cacttcgacg gcgtctgcac cgtggtggcg aagctcttca
     9721 acctggtgca gcccgatgag gcggtctttg gcaaaaagga ctggcagcag ctcgccatca
     9781 tccgccgtct ggtgcgcgat ctcgatttcc ccgtgcgcat tcacggcctc gacactgtgc
     9841 gcgagggcga cggcctggcg atgagctcgc gcaaccgcta cctcgcaccc gaagagcgcg
     9901 cccaggctcc cgcgcttcat gccgcgttgc gtgaggcgcg tgattgctgg ttgggcggca
     9961 gcgcatctgc cgacacgctc ctctctgtct tgcgcgggca tctcgcccgt tcggctccgc
    10021 tgggccaagt ggattacgcg gcgctcgtgg acaagcacac cctccagccg caggccgtgg
    10081 cggaccgctc cgggctgctg gcgctggcgg tgtatttcgg gaaggcgcgg ctcattgaca
    10141 acctggagtt ggacacgacg gcggagtgaa ttttcagcgt ttggcaaaaa tgaggtttcc
    10201 ttttggcggg caaattgcca gaatccgaaa aatctttatt tgctccctat gccacccctt
    10261 gtcggttccc agcttcttaa cgcgcgtctc gcgcgcggct ggacgctggc cgaagcggcg
    10321 catgagacaa gaatcccgga gcgccaactg gccctcatgg aggcggatga ttacgccgcc
    10381 tttggcaatc tggcctacgc cagggccttt ttgagaatct acagcaggca tcttgatgtg
    10441 gacaccagcg agcttctggc ctggctggcc ctgggcgcga gccgccccgc tccgctccgc
    10501 ctcgaaaaca gcaacagcgt gtggcacggt gctcctccgc cacaggccaa accccggcga
    10561 ggaccgctcc gcccgctcac gctcgccctg gccgtcctca ccctcatcct cggcggcgga
    10621 gtttacagcg gcatgcattt gcgcatggcc cggctgctga attttcccgg ccggcccgcc
    10681 tcggtgctgc cgcagcctcc gccggagcct gtgcccctgc atgcgatgga aaagacggcg
    10741 ctgccggact tgcggtctgc tggccgggga agttgaggcg caatgcgcgc tggcgcgccg
    10801 cgcggcgggc tgctagtctg gggcatgacc ccgaacggac tccccaaacg ctggtggctg
    10861 ccgccgctgc tctggacggc ggcctttctc gccgtgctca ttcccgccct cgttttcagc
    10921 gccacggcgc gcgaggtcgc cgccaagggc gtgatgaaca tcctcggact gctggccacg
    10981 cctttcatcc tcgaaaccgt caccgcgctg gccggactgg ccctggtcct cgcatggaat
    11041 caatggcgca ttcagaaaga cggcgacggc tgggtttacc tcgccaaaac ggaacccgat
    11101 ccggccagcc tggccgctgg tgcggacaaa gcggagcacc ggctggcggc ggtcgtgctc
    11161 gcggaaaagc cggactccgc gctcgatttc gaggcgcgtc tcgcggtggc cgagggctac
    11221 ctggagcttg gcctcggcag ggaggcgctg gagcacctgg atatgctcgc cccggatgag
    11281 caagcccacg agcgcgccgc cgcgctgcgg gtgaaagccg cgtccctggc agcttgagcc
    11341 gcctattctt gagcgatggc ccccgactca cagccatctg gcgcgggcaa ctggagaccg
    11401 ggccgctacc gcgtgacgcg ctgggttttt ccccgcctgc tggccgtggt ttacctctgc
    11461 gccttcgcct cctgggcggt gcaatgggag gggctgctcg gcgaggaggg catcctgccc
    11521 gtgccgcaga tcgtcgaaaa catccacgcc tacgaggcgc gggagcagac cacgctctgg
    11581 ctggagtacc cctccgtgct gcgctggcgc agtgacgacg cttttgcaca aatgctctgc
    11641 tggggctgct gcgggctggc tttgctggtc atggcgggcc tctggcaggg gccgctgctg
    11701 ctggtgctct gggccgccta cctctcgctg gcggcggtgg gtgaaatctt catgggctat
    11761 caatgggatg ccctgctgct ggaggcgggg ctgctcggtg tcttcgtggc gccgtggtcg
    11821 ttgcggcccg gcggactgcg cgccgcgccg ggtgatccac cctggatgtc cgtggtgctg
    11881 ctgcactggc tggtcttccg gttgatgttt ctttccggct tcgtcaagct ggcgggcggc
    11941 gacagcacct ggtgggaatg caccgcgctc ttgcaccact acgagacgca gccgctgccc
    12001 aacggcctct cctggtttgc gcatcatctg ccgcgctggc tgcacaccgc ctcgtgcgcg
    12061 gccatgcatg tcatcgagac ggggctgccc tttgccatcg tgctcgggcg ctggggcagg
    12121 cgcgcggcgg cgggcggttt cgtgctgctc atggcgctca tcttcgccac cgggagctac
    12181 aatttcttca acctcctcac cgccgcgctc gctctctgcc tgctcgacga cacaagctgg
    12241 ccgcgcaggc tgcgcgcctg gctgcggctt gatgatgggg cgcctccgcc gccgcgctgg
    12301 cactggcggc agtggcccgc gctcgccttc gcgccgctgt gcctgctgct gaccctggcg
    12361 gcggcggatt tgtttatggc cgggcgagtc tcctggcacc gcggcttcat gccggagtgg
    12421 gcgcgccagt tttacgcccg cacccagtcc ctgcgcagct tcaatgccta cggcctcttc
    12481 caggccatga ccaccgagcg cccggagatc atcgtcgagg tcagcgatga cggcgcgctc
    12541 tggcagccgc tggagtttaa atggaagccc ggccgccagg accgcgcgcc cggctttgcc
    12601 gccccgcatc agccgaggct cgactggcag atgtggttcg ccgcgctcgc ccccggattc
    12661 gatccgcgcc gcgactcgca gcccggctcc ccgctttact ggttcggccg ttttgccgcc
    12721 tgcctgctgg agaaaaagcc cgccgtctgg gcgctggtgg agccgccgcc cctgcccgtg
    12781 gaggacatcc gccacgtccg cgccaagctc taccgctacc gcttcagctc ccccgcccac
    12841 cgccgccaga ccggcgagtg gtggacgcgc gagtacctcg gcatgtggtc gcccgtcttc
    12901 agcgtgaagc gctgagccgc gcctgtcgcg cgagtttttt ttggcccgtt tcccgctttg
    12961 cattgtcaga caaaagccca cgccgccggc gttctacctt ccgtccatca tgagaccgat
    13021 tttcctgacc gcgctccttt tccccgccct gctcgccgcg gacctgccag acccgcccgt
    13081 cactttggag cggctccagg ccatccagga tgccgtgcgc gatctgttcc ccgccgcgcg
    13141 gcccgccgtc gtcgccatcc tcaccgggga cggcaccgcc agcggcgtca tcattgacaa
    13201 aagcggcctc gtcctcaccg ccgcccatgt cgcagagaca ccggggcgcg agctgcgcgt
    13261 tgtgctcgag gacggcaggc gcgtcaccgg caccacgctc ggcctcgaca aaaccaccga
    13321 cgccgccctg ctccagctca acgacaccaa gcgcgactgg ccccacgccg ccgtctcccg
    13381 ccgcgtcatc gaggcccagc ctggagagtg gtgcttcgcc ctcggccacc ccggcggctt
    13441 cgacaaggcg cgcggcgtcg tcctgcgcgt cgggcgcatc atccgccaga ccgccaactc
    13501 cctccagacc gactgcgtgc tcatgggcgg cgactccggc ggcccgctct tcaacctcaa
    13561 aggcgaggtc atcggcatcc acagccagat ctgggaaaag cgcgacgaaa acatgcacgt
    13621 ctccatggcc cccttcctcc gcgcctggga cgagatgaaa agcagcctcg tcatccgcac
    13681 ctggggcacc ggcagcggcg gctacctcgg cctcgccgcc gatgtcaacc ccgacaccga
    13741 actgctcgaa gtcctcgaag tcctcgccgg ctcaccctcc gaaaaagccg gcgtcctgcc
    13801 cggcgagagc atcctcgccc tggatggcga gccgctcggc ggcctctccc acctctccaa
    13861 cgccgtcaag ctccgcgccg cgggcgacca ggtgcgcctc cgccttaaaa aaggcggcca
    13921 ggagcgcgaa gtctccgtca ccctcggcca ccgccccaaa gaggaaggct gacccgccat
    13981 gcgccccgcc ctttgcctca tcctcctcgc cgccggtgcc tccgccgaga tgcgcccctc
    14041 cctccagccc gaggaccgca aaaacggcag ccgcaccctt ctcgccctcg accccgtgca
    14101 tcgccaggcc atcaaagcca ccgcccgcct cgtggacacc ggcggccgcg ccgccgcgct
    14161 cgccacctgg gtcggcgagg acggctacct catcaccaaa gccagcgagg cgcccgaact
    14221 cgaaaaactc caggtccgcc tcgccgacgg cagcctcgcc gccctgcgcg agatacggcg
    14281 cgacacccgg cacgacctcg tgctcgccca ggccatcggc gtcaccggcg tcaccgccat
    14341 ccccttcacc gccgacgcca aagcccctgc cttcggccag tggatcaccg cccccgccgc
    14401 cgacggccag ctccgcctcg gcgtcatcag cgccagctcc cggcagatcc ccggcctcgg
    14461 cgccgccatc ggcgtgcgca tggatgaaaa acccgccccc gggggcaaag gtgtccgcgt
    14521 cctcgccgtc gccgaggaca gccccgcccg cgccgccggc ctgcgcgatg gggacatcct
    14581 gctcagcctc gatgacgaca ccctcagcca gttcagccaa gtcagccagc tcatctccaa
    14641 gcgccagccc ggcgagatcg tcgcggtcaa atacctccgc gacggcaggg agcgcgcctg
    14701 caaagtccgg ctcgccagcc gctccaaagt cctcaacaac tgggagggcg aggacttcgc
    14761 caacggcggc atctccctgc gcaccgacag ctttgaaaaa atcctccagc acgacctccc
    14821 cctcggccct gccgacatgg gcggccccct gctcgacctt tccggccgcc ccctcggtat
    14881 caacatcgcc cgcgtggacc gcatcaccac cttcgccctg cccgccgccg tcttttggcc
    14941 tctggtcaaa ggctggatcg aagccgaccg ccaccccccc aaggccctgc cagccaggtg
    15001 actgcctggg atggaatgtg gaatgcgaaa tgggggaagt tcgaagctcg aactccgcag
    15061 tggaagcagc gttccgccgc ttttcgggtg ggggcggagc gcgcgcaagg agcggaaaaa
    15121 ggattgactg ccaaagcccg aatccagaaa ctcggttcat gaaatttctc tgtgccgcgt
    15181 ctctgatggc gctcgcttcc gccgccgcag ttcgcgccga tggcgtgccc tttgatcgcg
    15241 tgacaggacg agtgaccgtg ccgcacacag tgctgcatat gtccgccgag cagcgggcgc
    15301 aggtgtccca gacacgccgt ctcactctca cgccccggca gcatgagcgc cttgccaggc
    15361 tgtgccccgt gtttccgaat gagatcggcg aggtgttgtc gtatcgctac ggggactgca
    15421 cctgctgcgt cggtcatccc tacgccctgc tgcttccggg cggggcgagt gtggcggttc
    15481 cgcattcgga ggcggaggat gtatcgcgtt atggtgtgcg ggagcggcct tcctttgccg
    15541 ccaccacgac tgccggacag cgctcacgct ggttccggtt ctggagggcg gtcttccgcc
    15601 gggactgagc aaatccgcca cgccgccggc catgcttccc gcagcaactc caatccgccc
    15661 tcctgtctcc gcgctgctcc tcccaggcgg acggcatagc ttggacaatg ccaagacata
    15721 ctccccacca tgaagaagcc tgcaaccagt cgaaccgggt ggtggattgc cggcctatta
    15781 gagaaacact cgaacacgga ccggcctact tactggaaca attaccggct catcaaggct
    15841 ggtgactgga gaacggcatt ccgaaaggcc gccgaattgg gggcagctaa tgcgagggtc
    15901 ggaaacaaag ccttctccgg acatcaagag ttcatcgggg tcactgatct tctgccgatc
    15961 tacgacgaat tcgaggatgg tgcggaactg ctctggcagg aactggaagc ttcgcaagac
    16021 gacgatggaa ttcccttgag ggtgttcaca gtgactgatc tcgaatcaca gtacgaattg
    16081 ccgggcgatg atggcgttcc tgccaacgcc tgaccacttt cagttccagt catcaccctg
    16141 cgcctgtcgc catcaccgcg ccagcagcgc gcgtgtctcc tcgatgacct gggcgggcag
    16201 ggggtagtag ccgtctttga cgacaacgcg ctggccttcg cggctgtgaa cgtagcgcac
    16261 aaactcggcg gtggcggcat ccagcgggcg gccgggtttt ttgttcaggt agatgaggag
    16321 gaagcgcgcc agggggtagc tgccgttgat gcagttctca taggttggca gggcgaagcc
    16381 gccctcctcc tgccccaccg gcagcacgcg cacgccggag gtcaggtagc cgatgccggt
    16441 gtagccgatg gcgtgggtgt ccgcgctgac gctctgcacc acggaggagg agccgggctg
    16501 ctctttgacg ctgcttttga agtcgccccg acccagcaca tgctccttga aaaagccgta
    16561 ggtgcccgag gcgctgttgc gcccgtagag cgatacgggc cgccctgccc atccgggcac
    16621 gccgagcgcg gcccactccg tgatgtccgc aggcgcgccc attcggcggg tggaggagaa
    16681 ggcggcatcg agctgtgcca gggtgagtcc gcgcgcgggg ttgtccttgt gggcgaagac
    16741 ggccagcgcg tccactgcca cgcgcacggc caggggcgga tggccgaatt ttttttcaaa
    16801 ggcgtccagc tcctcctgtt tcatcggccg gctcatcggc ccgagctggc atgtgccggc
    16861 gatgagcgcc gggggagcgg tggcggagcc tttgccctcg acctggagga tcacttccgg
    16921 gtgccaggcc ttgaagccct cggcccacag ggtcatgagg ttgttcaggg tgtccgaacc
    16981 cacggagatg atgctgccgg agactctggc ggacgggtgg tactccggca gcgccgcgtc
    17041 cggctccacc gcgcgcagcg cgcccgagcc ggcggcgggc aggcagagga tggcggcggt
    17101 ggtcatgagg cttggcatgg atctgtcatt ctcccgcttt ccgtccgccc gcgcttgtcc
    17161 gcgcgggtga cagtttcgtc acgcatccca tgcccggcgc ggcggcggtt acaagaagga
    17221 aggttgccaa agcgcggccc acgctgacac tgcgcgccgc atggaagcca ccgcccccag
    17281 cacgcctctg tccattcgcg tgcgcgatct cgactttttt tacggccccc agcaggcgct
    17341 ggatggcgtg agcctggaca tgccgaggca gcgcgccacg gccttgatcg gcccctccgg
    17401 ctgcgggaaa tccactctgc tgcgctgctt caaccggatg aacgaccgca tcccggacgc
    17461 cgttgtgcgc cggggcggca tcgaggtgga ggggcgggat gtgctggcac ccggccttga
    17521 tgttgtcagc ctgcgccggg aggtgggcat ggtcttccag aagtcgaacc cctttccgct
    17581 gagtgtcttc gaaaacgtgg cctacggcct gcgggtgaaa ggcgagtcgc gcgcggatta
    17641 cctggagcat gtggtggagg agagcctgcg cgccgccgcg ctgtgggacg aggtcaagga
    17701 ccagcttcac agcgatgcgg gcgcgctctc cggcggccag cagcagcgtt tgtgcatcgc
    17761 ccgcgccatc gccctgcgcc cgcgcgtgct gctgatggac gagccctgct ccgcgctcga
    17821 cccggtggcc acggcgcgtg tggaggaact catccacggt ctgaagcggc gcttcaccgt
    17881 ggtcatcgtc acccacaaca tgcagcaggc catgcgtacc tccgacttca ccgcgttctt
    17941 ttacatgggc cgcctcatcg agcacgcgcc gactcaggcg tttttcgaga atccgcgcga
    18001 gaaaatgagc caggactatg tcacgggccg gtttggatga gaggttactt gcccggagtt
    18061 ttgaaaactt gcggggtcag ttcgttcaca gcgccggcga tgatctcctc gacgccggag
    18121 gcgaagcggg tggggcggcc ataatacgtc atggccccgg cggcctcgta gccgccctcc
    18181 tcccacacac gctgggaggg aatgtagcag ggcacgtcgt tggtgtaggc gttcacccag
    18241 gtgcgctggc gcccgaagga ttttttgaaa cgcagtccgt agtccacgac gacctcgccg
    18301 ggcaggtgga tcatgagcaa accgccgccg aagctccaga cctgcacgtt gtagggcagg
    18361 gtgtcgggga ttttttcgcc gcgctccatc atgccgagga aatgccgcgc gtgatgggcg
    18421 atccatttgt ttttgtcttg agtccgcatc tcccattccg ccctgccggg cagggtgtcg
    18481 aagggcagtt gcagatcctt gctccggcag atgaccggcc cggggagcgg cttcatgggg
    18541 ttgttgatcg cgtggatcac gcctttggcg agcgtgacgc catgcttgag ggcatgctcc
    18601 atctcgcgcc tgggaaaggg gttttgatcc gcgccgcagc caatggcggt gagggcgacg
    18661 acgccgggaa agcgcagctc cagctcggcc tgcgccgtgc cggcccagtc ggggtggatg
    18721 tggttgatgg cgagcgtggt gcagtggcag gcgtagctgg tgaaaagggc gcgcagtttt
    18781 ccctccatgt cacgcacgcg cagcacgggc agatcgtggt cgcgcacgcc ttcgaagttg
    18841 gggctgttgg cgtagccggt ctccgtcttg agccggcggt tcatggcgaa gccggccttg
    18901 cccgcacccc tgtccacctg tgcgggcttg aggtcgttga gcgcgctgag cgccacttcg
    18961 gtcattctgg cggtgaggaa ggcggtgtag cggtccacgg ccttccgctc gtcgggcggc
    19021 aggtccgcgc cgaacaaaaa gcccagcacg ccattgacca tgggcgcgca gtgggtgtgg
    19081 ctggagtgca gggagaaacg ctcgtcggcg agtttccatc cggcattgcg cacgttcgcc
    19141 agcacctcgg cgcgcatggc ggcggggatg ccgcagttgt ccaccgtgag catgaccacg
    19201 ggctcgtcag cctcccagcg aagggccagc gccttggcgt ggatgggcag ggaaacacct
    19261 tcgtgggggg agttccggct gccgtagccg ctgaggcgca cggggaaatc aggtgtcacg
    19321 tcagttttgg cggcgcccgc ctgccagcct tttgctttca aaactgcggg gatggaaagg
    19381 atgcagccag cacaggccag cactgcgagg aatttcattt ttccatgttt aatcagcatt
    19441 tttcaagatt gcgaaattac cccaggcaag ccaagcccga aatcatccgg cgcgagggcc
    19501 atgctggccg aaatcttgcg cccggcgcgg gcgtattgtc acgccccctc tgatgatgaa
    19561 aaactccccc tcctattccc gccgccagtt cctgcgcgga gcgggtgtgc tgctcggcct
    19621 gccgtggttc gagtccgtgt cctccttcgg cgcgcccgtg gtcaagggca ccccgcaggc
    19681 gccgcgccgg ttcgcggcct gcttttttgg caatggagtg aacccccacc actggggcgc
    19741 gggcaacggc gcgggcgggc tggaatttct ccagaccttg aagcccctgg agccggtgaa
    19801 ggaccaggtg atcgtgctca agggcctgtg gaaccccacc acggtggcgg ggccgggcgg
    19861 gcattacccg aagatgaaca tcctctccgg cctgaaggtg aagcagacca ccacggacgt
    19921 ggaggtgggt gtgaccatgg accagatcat cgccgccaaa gtcgggcggg agacccccgt
    19981 cgccagcctg gccatcggca ccgaggggcc gaaatacagc accgacagcg gctacacctc
    20041 cctttactcc gcctacctct cctggagcag ccccacgacg cccgcgccga aggagatcta
    20101 cccgcagcag gccttcgacc agctctttga cgacggcagc aagcgcgcgc gtgacaagag
    20161 cgtgctcgac ctcgtgctga gcgacgcgcg cagcctgcgc ggcaagctca gccggcgcga
    20221 cggccagaag ctggacgaat acctcgcctc cgtgcgcgac ctggagcagc gcatcgagcg
    20281 cgcggagaag ctcaacgccg ccgaggcgga cacgcgcggc tggcagccca gcgtgaggac
    20341 gccctggctc gcgcgccccg cctccggcat ccccgcgcgg caggaggagc atgtgcgcct
    20401 gatgctggac atcatggtgc tggccttcca gatggaccgc acgcgcgtca ttacaaacat
    20461 gctgacgaac gacctctcga acatgaactt cggcttcatc ggggtcaaag gcggccagca
    20521 cgagttctcc caccacgcca acgactccga gcgcctggac ggctaccaga agagcaacga
    20581 gttcatggtc caggcctggt ccgaggcgct gcaaaagatg cacgccacgc gggagggtga
    20641 gcgcaccctg ctggagaaca gcatgatcct gctcacctcc agcctctacg acggcaacgc
    20701 gcacgactcc acccagctcc cgcttgtgct ggcgggcggc ggcggcggca ccctgcgcgg
    20761 cgggcgctgc gtcgagttcg acaagaagga cgagaaccgc aagctctgcc gcctgcacct
    20821 ggccctcatg gagcgcatgg gcgtgaagac ggaccgcttt ggcgacgcgg acaattcgct
    20881 tggcgagttg gcctgagatt gggcgtgctc tccgaggggc caagcgcggg ctttcgggct
    20941 attgcagcaa tccgttcaaa gcctgtccgc tatacgcgac aaaaactgga atagcggtgt
    21001 agccatgcat tttgctttga gggggagccg cgggcaattg gccggcaaca ccgcaccgcc
    21061 ggcaaatggc gtgcaagttt gctggcggct acaaagcctt gaaaagggga tcaaccactg
    21121 tcggcggtag tcgcgtgcgg cgtcgttgcc aaaagtgtca gatgcccaag tgcccgtgag
    21181 ttgaccattc gagtcctctt ctcatgatga ctggcttgat gccattgtgc tagttcggcg
    21241 gcgttaactc agctccacgc agcccaatcg ccaaccgcgt gagcttcccc acgatgtcag
    21301 catgatcggc ggaaacatcg ttttgctcgg cgaggtcggt ctggaggtcg aagagcatcg
    21361 tcttcggcgt gaggtggagc ttccagcggc cttcacggaa ggattccagg cggcgttctt
    21421 tgtacccgta gagcgagtag agcgtggttc ggcgggattt ggcgtttgcg ttgaggaaga
    21481 ggtcagtttg gtcaatgccg tccagattcg cggacggttg ggtttcgcag aggcgggcaa
    21541 aggaaggcag gagatcaaga gtggtgagca tctcgccgca gacgcggttc gcgggcactt
    21601 tgcccggcca ccaggcgatg cagggcacgc gcaggccgcc ctcatagacg gtatgcttcg
    21661 tgccggtgag cggtgcggcg gtgccgggtg tcttgcgttc ggggccgttg tcgctggtga
    21721 aaatgacgag tgtttgttcc gcgaggccga gggcgcggag tttgtcgagg atctggccgg
    21781 tgctgtcgtc gagctcctcg acgacgtcgc cgtagaggcc gcgctgcgat ttgcctttga
    21841 acttcggcga ggcgtcgaag ggcaggtgcg gcatcgcatg accgaggtag aggaaaaacg
    21901 gccgctgacg atgctgcacg atgaatttca gcgcctcggc ggtgtagagc gcggtcatct
    21961 cggcgggcac ggcggggcgt ttctccaccg catcgccgcg cagcacgggc atgccgcctt
    22021 ccttttcaaa gacgaccgtt tcccagtggt cgaggttgtg cagcacgccg tagtagctgt
    22081 cgaagccctg atccaccggc cgcatgcctt tcacgaagcc gaggtgccat ttgccgatgc
    22141 agccagtggc gtggcccgct gccttgaaaa cctccgccag tgtcgtttcg gagcctgcga
    22201 gaccgttttt cgcatcggga cgcaaaacac ccgtcgcgag cccgacacgt cccggatact
    22261 ttccggtcat caacgcggcc cgggaaggtg tgcagagcgc ggaggtcacg ctgaagtcgg
    22321 tgaaccgcgt gccctctgcc gccatgcggt cgagatgcgg cgtgcggatg tcctttgcgc
    22381 cgtagcagcc gagatcgccg taaccgaggt cgtcacagtt gatgaggatg acattcggcc
    22441 cggcggcatg gaccgacaga ccaagaatca tcatgaggca ggcaagaatg ggcttcatca
    22501 ggtgcgaaaa cgaaggttgc ggcagacttc tcccgctggt ttcatcgccc gtgccaacca
    22561 tgaagctcat tcccaccctt ttgctcgccg catccgtctc cggcattgat agttgcagcc
    22621 cgcaacccag ctcgccgccc tccgacaagc ccaaggtcgc gctggtgatg aagtcgctgg
    22681 cgaatgagtt cttccagacc atggccgagg gcgcgaagaa gcatcaggcc gcgcatgccg
    22741 ccgactacga tctcgtcgtg aatggcatca agaatgaaac ggatctcgcc gagcaggtcg
    22801 gtttggtgga gcagatggtc gcgcagggcg tgcaggccat cgtcatcgcc cctgcggact
    22861 cgaaggcgct cgtcacagtg ctcaagcgcg ccaaggatgc cggcgtgctc gtcatcaaca
    22921 ttgataacaa gctcgacgcc gacacgctga agcaggcagg tctgcaaatt ccctttgttg
    22981 ggccagataa ccgggccgga gcgaaggcgg tgggcgaggt gctcgcgaaa aatctcaagg
    23041 cgggtgacga agtggccatc atcgaaggcg tgaccaccgc cttcaacggc cagcagcgca
    23101 aagcgggatt cgaagacgcg atgaacgccg ccggaatgaa gatcgtgagc tcgcaaagcg
    23161 gccagtggga gatggaaaag gccaatggag tcgcggcggg catcatcgcc gcgaatccaa
    23221 acgtgaaagc gctgctctgc gccaacgaca acatggccct cggcgccgcc gccgccgtgc
    23281 aggccgcggg caaggccggt cagatccaga tcgtcggctt cgacaacatc gccgccctca
    23341 aacctctcct cgccgacggc cgcgtcctcg ccaccgccga ccagcacggc gaccagcttg
    23401 ccgtcttcgg catcgaagcc gcgctcaaga ttctcaagga caagtccacg cctgcggatc
    23461 agcagacgcc ggtggatgtg gtgacgaagt gattttgtgc cccatggaaa aggtcttcca
    23521 acgaatttgc aaagggtatc gtgagcgccc ctgggcgagt ttgttctata gctttctgat
    23581 ctaccttttc attgcaatag cactcgtact ggccagcccc agtttatcag actcgactcc
    23641 aggaagactg actgatgaag aaaggggtga cggcattgtg ctgacggtga tcgtcctctt
    23701 tttggctggg ctgggcggtg gatggctcgc tgcgaagaaa tgatttcttg attcacccat
    23761 gcgccgcttc gctgactacc tcatcctcct cgccgtgctc gcggcgatga tcctgctgtt
    23821 cgggatgctg cgcgacagct tcctcagcgc ggcgacgctg ggctcggtgg cgaaccgcat
    23881 tccggctctc gctttggtcg ccacgggcat gacgctggtg atggtgacgg gtggcattga
    23941 tctctccgtc ggctccgtgc tcggactgtg cggcgcggtc gtcggtgtga tgatggccga
    24001 ctggcagtcg ggactattgc tcgccttgct cggcagcatc cttgtgggag gtgcgatcgg
    24061 cggcaccaca ggcgcggtca gcgtgaagct gcggcttccc tcgttcattg tgtcgctcgg
    24121 gatgctcgaa atggcgcgcg ggctggcgtt cctcgcgacg aactcgcaga ccaaatacat
    24181 cggtgcttcg attgagtcgc tgggtgctcc gtttggcggc ctggtcattt caccggcgtt
    24241 cgtcatcagc gtgctcgtcg tcgtcgcggg gcaggtgatg ctttcgcaga tgcccatggg
    24301 acgtcattgg atcgccatcg gtgcgaatcg tgaagccgcg ctcgtttccg gcgtccctgt
    24361 tgatcgtccg cgcattctag cccatgcgct gctcggcgct ttgaccggac ttgccgccgt
    24421 gttcaactgc tcccgcctcg gcagcgccga tccgaatgcg ggcgtcggat tcgaattgag
    24481 cgccatcgcc gcggtcgtcg tcggcggcac aagcttgatg gggggacgtg gcagcgtcgt
    24541 gaaaagcttc atgggcgtgc tgatcatcgc cacgctcgag gccggcctcg ttcaaatcgg
    24601 tgccacggag ccggtgaagc gcgttgtgac cggcgccgtc atcatcgccg ccgtgctcgc
    24661 ggacacctgg agacgcaaat aactcatgaa acgcctcatt ctctttctct gcgccttcag
    24721 tgcgtcgctc ttcgctgaga agccgaagat catcttcgat acggatatca ctggcgacgt
    24781 tgatgacgtg ctggcgctgg cgatgtgtca cacgctggcg gatcgcggag cgtgcgagtt
    24841 cctcggcgtg acggtttcga agaacaaccc gctgacggcg agctttgtgg atgcgcagaa
    24901 tacgtttcac ggccgcccgg acctgccggt cggtgtcacg cgcgacgaca aggcgcagca
    24961 tcgcgagagc aaatacctca agctggccga ttcggcggat tacccgcacg atttgaagcg
    25021 caacgaggac gcgaaggatg ccgtggagtt gattcgggag cttttgacgg cgcagccgga
    25081 tcacagcgtc acgatcatca gcgtcggcat cgcgtcgaat atggcgaacc tgctgaaatc
    25141 gcccggtggc gtcgccttgg tgaagcagaa ggttaaagcg ctgtcgatca tggccggtgc
    25201 gttcgcgtac tgcaacggca cgaactacca cctcgaagcg aacgtcatca acggcatcgg
    25261 ctacatgcaa accgtggccg atcaatggcc ggacgaggtg cccatcgtgt ggagcggcta
    25321 cgagatcggc gaggcgctgc cgtttccgcg tgtcgtgatc cagcgtgatc tcggctatgt
    25381 gccgcatcac atcgtcaaag aggcgtatct gctccacagc ggcccggagc atgatcggcc
    25441 gtgctgggac gagagcagcg tgctgtgggc cgtgtatccc gagcgcagct tttttggcct
    25501 ctccgagccc ggacgcgtca aagtgctcaa cgacggcttc acgcgcttca tgccgcaggg
    25561 caaagcccaa tccagcaagc gcgaccgttt tctcaccatg agctccgtgc agcaaagccg
    25621 cgcgctggaa gccatcgttc atctcaccat ccaacctccg aaaaaatgaa gcacctgctt
    25681 ttctcactcc tgttttgtgg tctcgcccag gctgcgcccg tgaagctcat cttcgacacc
    25741 gacatgggca acgatgtcga tgacctcatg gcgctgtgca tgattcacaa tctgcaaaag
    25801 cgcggcgcgt gcgagctgct cgccgtcacg gtgacgaagg atcatccgca ggcggcggcg
    25861 ttcgtggatg cggtgaacac gctttacggc tacccggaca cgcccatcgg cgtggtgcgc
    25921 aacggtgccg cgaaggaggc ggggaagttc aatctgctgg ccgacaagta tccgcacgat
    25981 ctcaaaagcg gcgcggaggc acctgaagcg ggcggtttga tcaaagacat cctcgcgaag
    26041 cagccggacg gcagcgtggt gatcgcgcag gtgggctttt tcacgaatct ggcgcgtctg
    26101 atggacgatg cggaggcgaa ggcgctcatc gcgaaaaaag taaaagtgct cagcctcatg
    26161 gccggcgcgt ttcagacggt gaacttcaac acccggcacc tcgaatacaa cgtgaagctc
    26221 gacatccccg ccgcgcaaaa gctggcgaag gaatggccca cgccgatggt gtggagcggc
    26281 ttcgagatcg gcgtggcggc ggcgttcccg catgtcgtca tcgagcagga cctcgactac
    26341 atgccgcagc atccgctgaa ggaggcctac tacctctaca acccgccgcc gcacgaccgc
    26401 cccacctggg accccacctc ggtgctgtat gccatttatc ctgaccgagg ttacttcgac
    26461 ctctcgcctc cggggaacgt gatcgtcgag gacgactccg ccacgctctt ccgccccacg
    26521 aaggacggca aaggcaaaca ccgcttcctc atcatgagcc ccgagcaggc cgcccgcgcg
    26581 cgcgaagcca tcgtgcagct cagcgtggag ccgccggcgg cttgaggggt gggcgcggca
    26641 gacgcattcg ccggaatgcg gggcttgggc agccggcggg attcaggccc gcgctttggc
    26701 aagcgcgcct acagcgcccc gtaggcgcat tcgccggaat gcggggcttg ggcagccggt
    26761 gggagtcagg cccgcgcttt ggcaagcgcg cccgcacccg cgcccttgcg ggcgcggcta
    26821 caccttgatg cccttcacct gcgcgtattc gggggaggcg gagccgtatt gggacttcac
    26881 actctcattg atggccttca tgcgcgcgcg caggccgggg gcgacgcgtt gctcgagcgt
    26941 ctgcagctgg cgcgacgtgc gggcgagcgc ctcgccgagc gagcatccgc gtgcttgaac
    27001 gcatcatcta cttcgagaac tacgttgtgg tcgacgcggg cgacaccagc ctgtcgaaga
    27061 tgcagacgtt gacggaagac gagtatcagg atgcgcgcga acagttcggc gaacgtttcg
    27121 tcgccaggat gggcgcggaa gcgatcaaga tgctgctcga agagattgac attgagcagt
    27181 tgagcgcgga acttcacacg cagctatcga cgacgacgag catgcaggcg cgcgcgaaag
    27241 cgatcaagcg cctgaaggta gtggaagcga tccgcaagtc gggcaacagc gcggcgtgga
    27301 tggtgctcga tgtggtgccg gtgattccgc ccgatctgcg cccgttggtg ccgctcgaag
    27361 gcggccgcta cgccacgagc gatctgaacg acctgtaccg tcgcgtgatc aaccgtaaca
    27421 accgcctcaa gcggttgatc gagttgcgcg cgccggaagt cattctccgt aacgaaaagc
    27481 gcatgctgca ggaagcggtg gacgcgctgt tcgacaacgg ccgccacggc cgcaccgtgc
    27541 ttggcgcggg caaccgtccg ctgaagtccc tgagcgacat gctcaagggc aagcagggcc
    27601 ggttccgcca gaacctgctc ggaaagcgcg tggactattc gggccgttcg gtcatcgttg
    27661 ttggccccga gttgaaactg caccagtgcg gtcttcccaa gaagatggcg ctggaactgt
    27721 tcgaaccatt catcattcgc gagttgaaag agcgctcgat tgccaccacg atcaaggcgg
    27781 cgaagaagat cattgcgcag ggccgcgaag aggtatggga catcctcgaa gaagtgatca
    27841 aggatcatcc cgtgctcttg aaccgcgcgc caacgctgca ccgtctcggt attcaggcgt
    27901 tcgagccggt gctgatcgaa ggcaaggcca tccgcattca cccgctggtg tgcgcggcgt
    27961 tcaatgccga tttcgacggt gaccagatgg ccgtgcacgt gccgctcatg ccgggcgcgc
    28021 agttggaagc gcgtctgctg atgctttctt cgacgaacat tttctcgccg tcgaacggac
    28081 gcccgattgt tacgcccagc caggacatcg tgttgggtat ttcgtacatg acgcgtctgc
    28141 gcaagggcgc caagggcgga tggaaaccgg agtggatgaa agacggcaag atcatcgagc
    28201 cgggccgcat tttccgcgac acggcgtccg tcgtgcttgc ctatgagatg gacgaagtcg
    28261 cgctgcacgc gcacgtcaaa gtgtggaccg acggcgagat agtcgatacc acggttggcc
    28321 gcgtgctgtt gaaagaagcg ctgccgccgg agatcgcgtt gaaagacgtg aaccgcgagc
    28381 aggacaaggg atccatcggc gacgttattg ccaatgcata cattacgcac ggccattcga
    28441 agaccgtcga gctgctcgac atgatgaagc gcgtcggctt caagtacgcg acgctcggcg
    28501 gaatttccgt cggtgttgtc gacatgcttg tgccggtcga gaagaagcag ctcatcgaca
    28561 gcgcgaaaca ggaagttgcg cgcatcgacg
//
LOCUS       DAOMYJ010000040        28589 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Prosthecobacter sp. isolate AMR_MDS_5629
            NODE_584_length_28589_cov_21.774690, whole genome shotgun sequence.
ACCESSION   DAOMYJ010000040 DAOMYJ010000000
VERSION     DAOMYJ010000040.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867850
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prosthecobacter sp. (wastewater metagenome)
  ORGANISM  Prosthecobacter sp.
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Verrucomicrobiaceae; Prosthecobacter.
REFERENCE   1  (bases 1 to 28589)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 28589)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.30
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:34:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,742
            CDSs (total)                      :: 4,684
            Genes (coding)                    :: 4,676
            CDSs (with protein)               :: 4,676
            Genes (RNA)                       :: 58
            tRNAs                             :: 55
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28589
                     /organism="Prosthecobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_584_length_28589_cov_21.774690"
                     /isolate="AMR_MDS_5629"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1965333"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            64..621
                     /locus_tag="PLP58_06375"
     CDS             64..621
                     /locus_tag="PLP58_06375"
                     /inference="COORDINATES: protein motif:HMM:NF023354.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3445 domain-containing protein"
                     /protein_id="HRJ08175.1"
                     /translation="MTKPDWNRIFSNEKHRLHMALRRSDLTSYWRGSNGGLLKERKKW
                     VEENQSRCLGVCDDASLQVSLALREIALLSEVDESRIERLAVEVVPDWTLLSRASGLW
                     RVVAGAVVFPSSWALNEKLGRPLFEVHGPVPGLNPTLGASIDIFLDSLTQFTAWERWN
                     WGLPPILNSIIIHPGICQDLLQVSG"
     gene            897..2150
                     /locus_tag="PLP58_06380"
     CDS             897..2150
                     /locus_tag="PLP58_06380"
                     /EC_number="2.6.1.66"
                     /inference="COORDINATES: protein motif:HMM:NF006964.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="valine--pyruvate transaminase"
                     /protein_id="HRJ08176.1"
                     /translation="MNDFSQTGRRLAGPSGIQELMDDLGGALALHPEMRMLGGGQPAA
                     IQCVQEIWRSRIAEMLADESTLDRALLNYDPPGGNPLFREAMAAFLRRECGWEVSLDN
                     IAVLPGGQTAFFLLFNLLAGETEGGKMRVIFPLMPEYIGYANQGLSADMLSGREPLIE
                     MRGAHEFKYRVDFDRLRVTSDTAALCVSCPANPSGNVLTDEEFDRLYEIALCHDIPLM
                     LDNAYGHPFPGVIHTDFRPVWRPGMIFSISLSKLGLPGLRTSVVVADAPVVHALRNMN
                     AIVSLANGNLGQAMLMPFLADDRLIRLGREVIRPFYQKRSEFTRQVLADSLGDNVPWA
                     VHSLDGAFFLWLWLKDSPVPSGELYQRLKARKVLVVPGHYFAFGMDGEWRHPQECLRI
                     TCSQPEAVVREGIEILADEVRRMWR"
     gene            2186..3181
                     /gene="pdxA"
                     /locus_tag="PLP58_06385"
     CDS             2186..3181
                     /gene="pdxA"
                     /locus_tag="PLP58_06385"
                     /EC_number="1.1.1.262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009960045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050570 - 4-hydroxythreonine-4-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxythreonine-4-phosphate dehydrogenase
                     PdxA"
                     /protein_id="HRJ08177.1"
                     /translation="MSKPIIAVTMGDPAGVGPEVCLQLLANEAVRDFATPVVFGDARL
                     LARCARQAGLITPKRIISEIEWPEKCANVDEPAVLDIFGFDAEDFTPGVVSARTGAAG
                     YRYVEKSIAAALAGQVAAVTTAPLNKEALRAAGVNYPGHTEIFAAKTGASRACMFQYS
                     AEVRASFVTVHVGYHEVPVLLTPERILDVIELTADAMQRIRGTRPKLAVLGLNPHAGE
                     HGLFGNREEEDIIIPAIEAARARGHAVEGPLPPDTAFIPAKRRATDAFICMYHDQGHI
                     PLKALAFDTAVNTTLGLPIIRTSVDHGTACDIAWQGKASGSSLVEAVRLAAKMAG"
     gene            complement(3207..4619)
                     /locus_tag="PLP58_06390"
     CDS             complement(3207..4619)
                     /locus_tag="PLP58_06390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009958163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1501 domain-containing protein"
                     /protein_id="HRJ08178.1"
                     /translation="MTSFTTRRHILQKAGAGFGSLALQSLLGRDGLLHGANPLSQRAP
                     HFTPKAKSVIFLFMYGGPSHVDLFDPKPALAKWHGKAIPVWKKEDAFMGGKTKNVAMR
                     SPYRFARHGQAGIDMAEVYPNLARHADSLCVIRSMHAESNNHGPALFQMNSGFIQAGR
                     PSMGSWVTYGLGSESENLPAFVVLLDKLGAPVNGALNWSNGFMPAAFQGVPFRGSGEP
                     VAYLTPPKSVSATRQRARLDLLRQWNAEFAASNPAETELAARLGAYELAYRMQISATE
                     CTDLAREPESVRGMYGLDHPVTAHFGKSCLLARRLVERGVRFVQVYSGGNEGPKAWDA
                     HDDLKKNHDLHCAETDGPIAALLDDLKITGLLDSTLVVWGGEFGRSPVAENGKGRDHH
                     PKGFTMWMAGGGIKGGQIHGATDEFGYAAVENRVSVPDLHATMLHLLGLDHEALTYRF
                     MGRDFRLTDVSGDVVKGLLA"
     gene            complement(4616..6601)
                     /locus_tag="PLP58_06395"
     CDS             complement(4616..6601)
                     /locus_tag="PLP58_06395"
                     /inference="COORDINATES: protein motif:HMM:NF019223.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1549 and DUF1553 domain-containing protein"
                     /protein_id="HRJ08179.1"
                     /translation="MRRLASPEIGPSGAGLIGWDEVREQWAMAFTLKTGLKVSPLHLL
                     SLCILTAQAKPPGMEEGRAHWAFQPLSQPRPPEVAQAGWLRNDIDRFILAKLEAAGLQ
                     PSPEADRAALIRRVTLDLTGLPPSPDEVEAFVNDDSLHAYERLIDRLLESPAHGERWG
                     RHWLDLARYADTSGFHNDLDRPHAWKYRDYVIRSFNEDKPYARFIAEQIAGDEIEGAD
                     ETSLIATGFCRNGPSNDDNMGKTPEALAQYRADQMDDVISTTASVFLGLTLGCARCHD
                     HKTEPLSARDYYSLHAVFAGTERLGLPKGATDARDKAVSDDARVMALIETKPDAPPTH
                     VMLRGLAASMGDEVLPAAPAVLGGRSLNPERPNGRTSMRRGTLAEWIASPENPLTWRV
                     MVNRVWQHHFGAGLVATPSNFGLSGAAPSHPDLLDWLAARFMAGGGRLKPLHKLMLMS
                     AAYRQQSSSHARGAAADPANTLLWRMNLRRMEAEVIRDGILAASGNLNPVMGGPGIKP
                     RIRADLLDASQRNKWPVLAQEGPGEWRRSVYIYVKRQLLMPSMELFDAPTTTDSCAMR
                     QRSTVPTQALVLMNDEFVEEQAGHLARRAAAGAREDLPRVIERLFMLALSRAPTAARM
                     EQALAFIGERALSAGTEEALRDLAHVLLNSSEFVYIP"
     gene            6679..8064
                     /locus_tag="PLP58_06400"
     CDS             6679..8064
                     /locus_tag="PLP58_06400"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase"
                     /protein_id="HRJ08180.1"
                     /translation="MNVLFIAIDDLGNALGCDGAAGARTPHMDLLAASGVRFDRAYNQ
                     MPLCNPARASLLTGRRPDAAQVYDLDRHFREALPDVVTLPQLFRQNGWLSARAGKIYH
                     YDVPKGIGTDGLDDRPSWDVVVNPKGRDAHDEALIINPTPEKPVSAALSWLAADGADE
                     EQTDGMIATEAIALMEKHAGRPFFLSVGFFRPHTPFVAPKKYFEMHPLESIRLPDAPA
                     DDRADIPVAAFAHNNPVPDYGLDELACRRALQAYRASVSFVDAQVGRVLDALERLGLA
                     ENTIVVLWSDHGYHLGEHLGVWQKRTLFEEGARAPLLIRAPGRAGNGRVCGRVVEFVD
                     IYPTLADLAGLKPATPLDGRSLVPLLDDPARAWDHGAFTQILRPADDRLPAQVMGRTV
                     RTERWRYTEWNGGKEGLELYDHGDDPREFRNRALNPDAEAVAAMGRLRALFEGRARAL
                     PPDSPVNAKRL"
     gene            8131..9180
                     /locus_tag="PLP58_06405"
     CDS             8131..9180
                     /locus_tag="PLP58_06405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009963457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000976 - transcription cis-regulatory
                     region binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HRJ08181.1"
                     /translation="MSAESPATLKDIARAAGVSMMTVSRVLRGAPRVSAEKRELVLGE
                     AERLNYRPDPHLARMMQAVRARKQKRVRAVIAVIREHVPQDGLLSPAYQYVQVEDIRR
                     RAHGHGYAVEEFFLGRDGLTPKRLQKILHARGIEAVIVSPQSTQLPCSRLDYAPFAAV
                     TFGYAMSSPALHMCAGNMTLGIQTAARELARRGCRRIGVAVTKWIVNRSQFGYSGGLF
                     HWQQDLYPEQRVPLLLFPDNDISRGFEPFAEWMRRHRPDAVISFDAHVPGWLKRLGLR
                     IPGDICFVAHDWTPRMRGFAGIYQQRDHLAAAAVDLIVTQLSQHEHGVPEVPRQIMIP
                     PRWVDGPSVRALPLA"
     gene            9847..12606
                     /locus_tag="PLP58_06410"
     CDS             9847..12606
                     /locus_tag="PLP58_06410"
                     /inference="COORDINATES: protein motif:HMM:TIGR02604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HRJ08182.1"
                     /translation="MRLLFSCLVLFHATAFSSDFPAPYNSEPGNPSPMSPQEALKALK
                     LPEGFKATLFAAEPDVQNPIAMAWDAKGRMWVAENYTYAERAKRFDLTLKDRVIILED
                     KDWDGIAETRKVFTEDVQMLTSVEVGRGGVWLMCPPQVLFIPDANGDDIPDGPPQVVL
                     DGFTVAEANYHNFANGLRFGPDGWLYGRCGHSCPGNLGVPGTPDDKRVPMKGGIWRFH
                     PEKKIVEVLTHGTTNPWGHDWDANGEMFFINTVTGHLWHLIHGAHLVDSNTPNPLIYE
                     KLDTIADHYHYDRTGAWSDSRDGKANHLGGGHAHIGMMIYKADQWPEQFRNKLFTLNM
                     HGRRANVERLERSSSGYVGKHEPDVFLSDDPWFRGIEISTGPDGSGFILDWSDTGECH
                     ESTGVHRTSGRIFRISYGDPKMPEKPFAWRKPWPKAELKSPDEYARVLAIRELTQFMP
                     LDTIIGPMPGSDYFKHVPTDVLKLLEMTAVNDKSSLVRLTLASTLQRLPVSRRQNLAA
                     ILLSHTEDANDPFLPHMIWFGISPLKAEALLEIAHSCSWPKVNRWIARKLATDPEALN
                     QLLAINSSDDVLEGMAEAFRGIRKAPKPANWDKVVSTSKSPYVRDLSILFGDGRALDD
                     VKKLALDAKADPAAREAALRTLIEARPDDLREVCTKLIEVRGLNTTAARGLALFNDPE
                     IGRQLAKNYRKFPGGIMDTLVSRPAFAQAMLAEMAAGRIPRTELSAFHARQIRSFKDE
                     SLSKQLVEAWGELRESAADKKQLIEKLKADLKPEVLAKASLGQGRVLYSALCGACHML
                     YGTGGKIGPDLTGSGRSNLDYLLENIADPSAVVSADYRMSVLTLKDGRILSGVIAEKN
                     ERTVTLRTLTESLTLDRAEITKTETSPMSMMPEGLLLALQADQVRDLIAYLMHPVQVP
                     LPDGR"
     gene            complement(12657..13085)
                     /locus_tag="PLP58_06415"
     CDS             complement(12657..13085)
                     /locus_tag="PLP58_06415"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009965982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HRJ08183.1"
                     /translation="MSAEEIPTIETREEVMFFDTDIGGVVHNIAYLRMIETARTRLAA
                     RLGMKLRDMARTQVYPVVVRTEIDYRKPATLGDELVITGRLDRLERVRFWVAFEIRRA
                     GEDAVLITCRQSLALVQMPQGRPLRLPEDWTVKYAPVFLK"
     gene            complement(13140..15668)
                     /locus_tag="PLP58_06420"
     CDS             complement(13140..15668)
                     /locus_tag="PLP58_06420"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF019334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional serine/threonine-protein
                     kinase/formylglycine-generating enzyme family protein"
                     /protein_id="HRJ08184.1"
                     /translation="MSDPSRTPPEIRDHETLRMIGRGAYGEVWMARSVTGALRAVKVV
                     WREDYDHADSFEREFEAIKHYDPVSRKHPGLVPILQVGRSDEQGFYYYVMELADDLER
                     GRDIKAETYKPHILGLQMRRQKRLKAGDCLKLGANVAEGLHHLHQHKLIHRDVKPSNL
                     IFIDGQCRLADIGLVALMGQRSFVGTEGFVAPEGPGSPESDIFSLGMVLYEASTGKDR
                     LDFPDIPSCTETGDQLEDWQRLHKVICRACAPRAKDRYSSAWEMALDLRGQPLPSRRA
                     VWQWAAAAVVTLALAVGFGMWLAMREGGALVAVQSSTPLLTISTEPPGAEVFAGEKLL
                     GVTPLEINPEEGVSVIYQLRMTGHKQMELEHIATRAQPARFDVKMEATRLPQPGERWT
                     NSLGMTFKPVLNGHTTTEPVQIKHFKRFLDDTGRSFEGRVVRAGQNKESAYFIVAPIG
                     DAEAFRFWLTEEDRRAGHLSQEHQYQVEPFYYVETGASSEDMPDDRDSTEGASQESQD
                     WQAFNLRVERQSYGSVVIRTMPEKVRVFQHEQFLGYTPLELPRVRSGSAEYDLREEGF
                     SDVILEGEVRPGELLELFADMQTRQAVTFGREWTANSLGMRLVPLGESVMIAATETRR
                     RDYLEFAKAANRRRPGNLDADGRGATQPVVSVDREEARAFCAWLTERERNSGLIGPAD
                     TYRLPTDEEWSRAVGLPLERGVTPAERNGRIRGIYPWGFDWPPPPKTDNFADIGAMRK
                     GNLENIIPGYEDGFPQLSPVGAFPANERGILGLAGNVSEWVDTDFDNGRKAAAGSKAV
                     LATARGGNWRSASADELLASARASHPADHRSPALGFRVVLGRSR"
     gene            15833..16303
                     /locus_tag="PLP58_06425"
     CDS             15833..16303
                     /locus_tag="PLP58_06425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009962800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SUF system NifU family Fe-S cluster assembly
                     protein"
                     /protein_id="HRJ08185.1"
                     /translation="MPISDDLRDLYQQVILDHSRHPRNYGELAGDCAHAHGDNPSCGD
                     EVDLWVRFSPYGSIEDIKFSGQGCAISQASASMMTVKVKGLTKEKAAAMIEDFRHVVT
                     GEGAVRDEDALGELQLLEGVQKFPQRVKCAMLAWRALEQALAGARVNPEWGRHV"
     gene            complement(16275..17795)
                     /locus_tag="PLP58_06430"
     CDS             complement(16275..17795)
                     /locus_tag="PLP58_06430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009963964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase"
                     /protein_id="HRJ08186.1"
                     /translation="MKLAASLLAAALAFSCSLLAQEKRPNVLFIAIDDQNDWIGHLGG
                     HPLAKTPNLDKLAARGTTFLNAHCNAPLCNPSRTSLLLGLRPTTTGIYGLAPWFRTLP
                     EWKDRVALPQHFANNGYRTAATGKIYHGGTGGRGAGPKARARGGDQGPPEFQVSAPYG
                     GVGTKPPKKLIPPTPMGDHPLMDWGVWPLDNDDTQKGDYQVASWTVEQIKNAPKDQPF
                     FLAAGFFLPHVPCYATQKWFDLYPDDDSVLPKTLEGDRDDTPRFSWYIHWNLPEPRLK
                     WVKENNQWRNLVRSYLACTSFVDAQIGRLLGALDEAGIADNTIVVVWGDHGWHLGEKA
                     ITGKNTLWDRGTKVPLIFAGPGVTAGQRCTQPAELLDIYPTLVELAGVPARDDLEGLS
                     LAPQLKDAATKRERPAITSHNQGNHGIRSERWRYIQYADGSEELYDMQADPREWHNLA
                     GKPEHAAVIAGHKKWLPKVDLPPAPNSASRVLTYDKETDTAIWEGAPVKRGDPIPD"
     gene            17928..19211
                     /locus_tag="PLP58_06435"
     CDS             17928..19211
                     /locus_tag="PLP58_06435"
                     /inference="COORDINATES: protein motif:HMM:NF012783.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease proteolytic subunit"
                     /protein_id="HRJ08187.1"
                     /translation="MKIHLLLLPLLFCSLSTLTGAEEKPVDAPAVESKKDEPADPLAG
                     LKAETARLEAERSKITAQLALEQARLDEKLAGQKRAVAEIQAQMEDLKTRMDLAEIKR
                     RSADDPELLELRRKSERLVLEAVIAKNEVELQSFEMRKKENEVRARTSEITLQIELQQ
                     KEAEARTHAVGKAPEYLKDPHQGGKLVISDRRIALNGVITAKTADHIADRIAYFNNRD
                     SEAPIFIVIDDCPGGSVMAGYKIIKAMHGSKAPVHTVVKSFAASMAACITTVSKKSFA
                     YPNAILLHHQLSVGVAGNLTQHRENVKQLEEWWRRLADPVAEKMGLTREEFIARMYQE
                     ASTGDWSEFADNARKLKWVDEIVEDIEETSLLRHPDSQQTASTTSAPRLPGMPPQTEI
                     IESNDERGKPIALLPRLNPLDAYWLYNPDGFYRLQ"
     gene            complement(19271..19765)
                     /locus_tag="PLP58_06440"
     CDS             complement(19271..19765)
                     /locus_tag="PLP58_06440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ08188.1"
                     /translation="MDKRGGAMNLFISSSRVLAALIFMQAVLPLEVDAKSGEELRAMY
                     TKFLKKEGYQPEVDGDGDVVFKHEGKTYFIDVHDKDSEFFMIALPNIWPIESSVERRK
                     VLAAADETNQETKMAKIYTKGDNVWVTAESFVAIPEDFEDVFKRMLSSIVTAVDLFAD
                     KMRE"
     gene            19914..21299
                     /locus_tag="PLP58_06445"
     CDS             19914..21299
                     /locus_tag="PLP58_06445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007415055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase"
                     /protein_id="HRJ08189.1"
                     /translation="MPKLLPRLILYTCVFAAFSQVKFPAAPPNVVILFADDLGYGDLG
                     CQGSPVIRTPNLDRMAAEGLRFTDFYSAAEVCTPSRAALLTGRYPIRSGMCETPGARR
                     VLFANSKGGLPQSEVTLARALKARGYATAHVGKWHLGIHEGGRPLEHGFDSSFGLPYS
                     NDMDGRPGLPKGSSGSPNPPQDGWNVALMRDGKVVEQPADQTTLTRRYTEEAVKFITE
                     KKGGPFFLYLAHTFPHVPMFASPQFKGRSRAGIYGDAVEELDWSTGQVLDALRKEGLA
                     GNTLVFFTSDNGPWLIMGSQGGSAGLLKDGKGSTWEGGMRVPGIAWMPGRVQPGVTSA
                     VAHMMDLFPTALALAGVEKPAGVTLDGVDLAPMLFAGRSLPERPFFYYRGDQIFACRL
                     GEWKAHFKTQTGYGQPKAEAHERPLLFHLGLDPSEKRDMAATHPDVIARIQDAVRTHQ
                     AAMTPGAPQLQ"
     gene            complement(21339..21989)
                     /locus_tag="PLP58_06450"
     CDS             complement(21339..21989)
                     /locus_tag="PLP58_06450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007418719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HRJ08190.1"
                     /translation="MLKHSRAGFTLIELLIVITIIALLFGLTIGGFTYAQRFANRSKT
                     AATIKAVQSGLERYNTEFGEYPEPASPGDTISIQDKTYTVSGASMLYQAMSGDGYDAI
                     KIAVPPANAGNPQSNGQVEADESRNTMITDMPKDIWRELEGRFFLIDGFSKPIQYVKA
                     LPQNLTGGAGANPTTINTHYDLWSYGEDEENTMSTSIESNAGGPLRQASEKWIKNW"
     gene            complement(22041..23318)
                     /locus_tag="PLP58_06455"
     CDS             complement(22041..23318)
                     /locus_tag="PLP58_06455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018968689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HRJ08191.1"
                     /translation="MPKFLYIALDQNGQEVTGELDAANEAEAVNLLRQSQLYPTQVAQ
                     EGRGDAAVKKAAKTTAAKSKGKTAVKAGANAKVKPKTLMIFTRQLATLIDSGLPLLRG
                     LTVLSRQEPNPVMKSTVSTIADNVQTGSTFSETLSQYPKIFNKLYINMVKAGELGGVL
                     EIVLNRLAEYQEKAQKLKNKVVAAMVYPIIVMFIALAIMVFLMLVIVPRFEKIFEDML
                     GDKNKLPELTKWVIGFSRWMGDNVLWLGLGIVVVVVVWKMFASTPGGRRSIDSMKLKL
                     PLFGDVQRKTAISRFTRTLGTLVTSGVPILQALNITRETAGNTVVSEAITKVHDAVKE
                     GESMVGPLEASNVFPPMVISMVDVGEETGQLPEMLLKIADVYEDEVDNSVSALTSMLE
                     PLMIVLLALVVGVIVMALFLPLIEVIKGLSAGG"
     gene            complement(23433..25091)
                     /locus_tag="PLP58_06460"
     CDS             complement(23433..25091)
                     /locus_tag="PLP58_06460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009964144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GspE/PulE family protein"
                     /protein_id="HRJ08192.1"
                     /translation="MTAQNVVDMLEARGVIDSGQAYDLTQDVVQNGKDILQALLDYEI
                     FPNEDVFWSLVAEELGAEHFDLASFEPAPSVVNLIPGGMARLYGAFPVTLDGRGLHVA
                     FTDPLNPQLVEDLRFGLDKIIVPVVARRSQVQDLIDKHYGTSAPNIDEIFGGLKDAGK
                     NAPEVEANSAPIVKFVDLVMTQAIKERASDIHFEPFEHEFKIRYRVDGALYEMAPPPV
                     HLATSVISRIKVMSNMNIAERRIPQDGRIMTVVNGKAVDMRVSSLPTQHGESVVLRVL
                     DRSSIQLELTDLGMPGPLRDYIEDTIEKPNGIFIVTGPTGAGKTTTLYACLRKINTVD
                     SKLLTAEDPVEYELDGIMQVPVNDAVGLTFARALRAFLRQDPDRIMVGEMRDKETAQI
                     AIQASLTGHLVLSTLHTNDAAGAITRLVDMGVEPFLVAATLEGVLAQRLLRTICKNCR
                     APYEPSQSILNQLNLSADDIGGKHFYTGNGCDKCGGSGYKGRKGIYELLDVTDPIREL
                     ITQKAPTLVLKQKAIELGMQTLREDGLRNIYEGETTIEEVLKYT"
     gene            complement(25140..26792)
                     /locus_tag="PLP58_06465"
     CDS             complement(25140..26792)
                     /locus_tag="PLP58_06465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007416796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GspE/PulE family protein"
                     /protein_id="HRJ08193.1"
                     /translation="MYSNEEYLLELLTEAGLVMESDLQKVKTTKKPNESTLEGLIKTG
                     VVSDEQVAQTVAVNSGMEFIDLHGFAASPQLKTLVPMEVARKYKIVPVGMNGPAMQIA
                     VSDPYDFETLDALPHVIQPDLEYFCSTPALISALQLNVYGNEFVTDASSAAAGGMKSD
                     DAPVIKLVNNILLEAFKNGASDIHIEPLEKDIRVRLRIDGVLHDVEHHPKRLHSAIIA
                     RVKIMCGTMSVDEKRVPQDGRIQMAFQEKELDMRVSIIPTNNGESVVMRVLDKSSLRL
                     GLSDLGFLSDDQDTFEKLITLPDGIVLVTGPTGSGKTTTLYACLNFINRPDRKIITVE
                     DPVEYELAGINQVMVKEDVGMTFGAALKAILRQAPNIIMIGEIRDLETASIAINASLT
                     GHLVFSTLHTNDAPSSIARLADIGVKPFLIASAVRGILAQRLVRKLCGTCKEPTSLNE
                     REMRHLGLEASQLYSANIMGPKGCNKCRGAGFKGRMVIAEIFKVDDEVRNMVNMQLTT
                     PQLRKRARELGMRTLREDGIRKVMAGMTTAGEVIEATMADAE"
     gene            26988..28034
                     /locus_tag="PLP58_06470"
     CDS             26988..28034
                     /locus_tag="PLP58_06470"
                     /inference="COORDINATES: protein motif:HMM:NF023431.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3500 domain-containing protein"
                     /protein_id="HRJ08194.1"
                     /translation="MIRHLLSTLVLLSTFAAPAHRLGAHEAGAQMAQVAAVFLASLSP
                     EQKTRATFAFEDAERVNWHFIPRERNGLPMKDMTPQQRLLAHALMNTGLGFRGSAKAA
                     TIMSLEEVLYQIEGADESKRAAVRERRDPEKYFVSIFGTPDVKGAWGWRIEGHHLSLN
                     FTIKDGRLLRATPAFMGSNPGELRQGPLTGLRVLGREEDLGRELVKSLTGEQFQKAVV
                     ADVAPKEMLTAAEQRVDPLKPDGLSAAEMTDAQKAKLNEIIQEYLGRVRPEIAQETWD
                     EIGKNGPVFFGWAGGRERGEPHYYRVQGATFLIEYDNVQGEANHPHSVFRSFDGDFGR
                     DLLGEHHREQHGKK"
     gene            28059..>28589
                     /locus_tag="PLP58_06475"
     CDS             28059..>28589
                     /locus_tag="PLP58_06475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ08195.1"
                     /translation="MLRHALFAALLPLAAAAAPEIPLAHGPHRDLALQALPDSTLEIR
                     LGGGHPHFWTAVVPAGYDPARQSILALDYFAPSGLESVVLRYRAQGGDMVVAEARQTG
                     IAEAWQPLVFDLSRLDPPPAAGHPEMRFHFALNGAAESLLRLRHLRLRAPTAAEARLA
                     AERDQTLAAREADAAAI"
BASE COUNT         4945 a         8683 c         9479 g         5482 t
ORIGIN      
        1 gcggggggtt gttgctttac gcgcacaact acccgtcttc gtccgcacgc tacttcattt
       61 gtcatgacca agcctgactg gaatcgcatt ttctcaaacg aaaagcaccg cttgcacatg
      121 gccttgcgaa gatctgatct cacaagctat tggcgcggct cgaatggggg acttctcaaa
      181 gaaagaaaaa aatgggtcga agaaaatcag tccagatgtt tgggcgtttg tgatgatgcg
      241 tcgttgcagg tttcgcttgc cctgcgagaa attgcccttt tgtcggaggt tgacgaatct
      301 cgtattgaaa ggctcgcggt ggaggttgtg cctgactgga ctctgctttc aagggcttcc
      361 ggattgtggc gtgtcgtggc aggagccgtt gtcttccctt catcgtgggc gttgaacgaa
      421 aaactcggga ggcctttgtt cgaagttcat ggacccgttc cgggcttaaa ccccacgctt
      481 ggggcatcca ttgatatttt tctggacagt ctaacgcagt tcaccgcctg ggagcgttgg
      541 aactgggggc tgccgccgat tctgaactca atcatcatcc atccaggaat ctgccaggac
      601 ttgctccagg tgtccggata aacaaagttt gggttcgttt ggaaaaccag catctctgtc
      661 ttcttcaatg tggactcatt ttatttggaa tccgcgtgag ctgtcatcgt cttgacaaac
      721 tggtggaaaa tgttcctggg ttggctccac gcatcgcaaa ctctctcgaa acaatgatgg
      781 aggatgtggc cctttacaag ggcgtctcgg atgctcgcgc cggaatcata tcggctttgc
      841 gcgccatttc gggaaattct tgacgcttgt ccagtcgagg cgtttccatg actggaatga
      901 atgatttttc ccaaaccggg cggcgcctcg ccggaccaag cggcatccag gaactcatgg
      961 acgatcttgg gggcgctctt gctttgcatc cagaaatgcg aatgcttggc ggcggccagc
     1021 ctgccgcgat tcagtgtgta caggaaattt ggcgtagccg tattgccgaa atgctggccg
     1081 atgaaagcac tctcgaccgc gcattgctaa actacgatcc tccgggcggg aatcctctat
     1141 ttcgcgaggc catggcggct tttcttcgtc gtgaatgcgg ctgggaggtg tctttggata
     1201 acatcgctgt actaccagga ggtcagactg ctttttttct cctgttcaat ttattggcgg
     1261 gtgaaacgga gggcggaaaa atgcgcgtca tttttccgct gatgcccgaa tacatcggtt
     1321 acgcgaacca gggcttgagc gcggacatgc tttccggacg cgagccgctc attgagatgc
     1381 gcggtgcgca tgagttcaag tatcgggtgg atttcgaccg cttgcgcgtc acgtctgata
     1441 ctgcagcttt gtgtgtttcc tgtcccgcca atccttccgg caatgtgctg acggatgagg
     1501 agtttgacag gctttatgaa attgctcttt gccatgacat cccgctgatg ctggacaatg
     1561 cgtacgggca tccctttccc ggcgtcattc acactgattt ccggcctgtg tggaggcctg
     1621 gcatgatttt cagcatcagt ctttcaaaac tggggctgcc tggcttgcgg acatccgtgg
     1681 tggtggcgga cgcgcctgtt gtgcatgcct tgcggaacat gaacgccatt gtctccctag
     1741 ccaatggcaa tctcggccag gcaatgctca tgccttttct tgctgacgac cgattgatcc
     1801 gtctggggcg ggaggtgata cggccctttt atcaaaagcg ctccgagttc acccgccaag
     1861 tactcgcgga ttctctcgga gacaacgttc cctgggcggt gcattctttg gatggcgcct
     1921 tctttttgtg gctctggctc aaggactcgc cggtgccatc aggggagctt taccagcgtc
     1981 tcaaggcgcg caaggtcctc gtcgtgccgg ggcattactt tgcctttggc atggacggtg
     2041 aatggcggca tccgcaggaa tgcctgcgca tcacctgcag ccagccggag gctgtggtgc
     2101 gggagggaat cgaaattctc gccgacgagg tgaggcgcat gtggcgttga cggattcgct
     2161 ggagcatccg atttctgcct ctgacatgtc caaacccatc atcgccgtca ccatgggcga
     2221 ccccgccggg gtcggccccg aagtctgcct gcaactgctc gccaacgagg cggtgcgcga
     2281 ttttgccacg ccggttgtct tcggcgacgc gcggctgctg gcacgctgcg ccaggcaggc
     2341 gggtcttatt actccaaagc gaatcatcag tgagatcgag tggccggaga aatgtgcgaa
     2401 cgtggacgag cccgccgtgc tggacatctt cggctttgac gcggaggatt tcacgcccgg
     2461 cgttgtcagc gccaggacag gagccgctgg ttacaggtat gtggaaaaga gcattgccgc
     2521 ggctttggcc gggcaggtgg cggcagtgac caccgcgccg ttgaacaagg aagccctgcg
     2581 cgccgcaggc gtcaattatc ccggccacac cgaaattttc gccgccaaaa ccggcgccag
     2641 tcgcgcctgc atgttccaat acagcgcgga ggtgcgcgcc agttttgtca cagtgcatgt
     2701 cggctaccat gaggtgccag tacttctgac ccccgagaga attctcgatg tcatcgaact
     2761 caccgccgat gccatgcagc gcattcgcgg cacccggcca aagctggcgg tgctcggact
     2821 caacccacat gcgggcgagc acgggctttt tggcaatcgc gaggaggaag acatcatcat
     2881 cccggccatt gaggccgctc gcgccagggg gcacgccgtg gaaggcccgc tgccgcccga
     2941 caccgccttc atccccgcca agcgccgcgc cacggacgcc ttcatctgca tgtatcacga
     3001 ccaggggcac ataccgctca aggcgctggc ttttgacacg gcggtgaaca ccacactggg
     3061 gctgcccatc atccgcacca gcgtggacca cggcaccgcc tgcgacatcg cctggcaggg
     3121 caaggccagc ggcagcagcc tggtggaagc tgtgcgcctg gcggcgaaaa tggcgggctg
     3181 agtgtcgcgg cgggcgcgcc gcgcgctcat gccagcagcc ccttcaccac atcaccgctg
     3241 acatcagtca ggcggaaatc ccggcccatg aagcggtagg tgagcgcctc gtggtcgaga
     3301 ccgagaagat gcagcatggt ggcgtgcagg tcgggcacgg agacgcggtt ttcgacggcg
     3361 gcgtagccga actcgtcggt ggctccgtgg atttgcccgc ctttgatccc gccgccggcc
     3421 atccacatgg tgaagccttt tggatgatgg tcgcggcctt tgccattctc cgcgacgggc
     3481 gagcgcccga actcgccgcc ccagacgacg agcgtggagt cgagcaggcc ggtgattttc
     3541 aaatcgtcca aaagcgcggc gatggggccg tccgtctcgg cgcagtgcag atcgtggttt
     3601 ttcttcagat cgtcgtgcgc gtcccacgct ttcggcccct cattgccgcc gctgtaaacc
     3661 tgaacgaaac gcacgccgcg ctcgaccagc ctgcgcgcca gcaggcagct tttgccaaag
     3721 tgcgccgtga ccggatgatc cagcccgtac attccgcgca cgctttccgg ctcgcgggca
     3781 aggtcggtgc attcggtggc gctgatctgc atgcggtagg ccagctcgta agcgcccaga
     3841 cgcgcggcca gctcggtctc ggcagggttg gaggcggcga attcggcgtt ccactgccgc
     3901 agcagatcca gccgcgctcg ctggcgggtg gcgctgacac ttttcggcgg tgtgaggtag
     3961 gccaccggct cgccgctgcc gcggaaaggc acgccctgga acgcggctgg catgaagccg
     4021 ttggaccagt tcagcgcgcc gttgaccggc gcgccgagct tgtcgagcag gacgacaaag
     4081 gcgggcaggt tttcgctctc cgagccgagg ccgtaggtca cccacgatcc catgctggga
     4141 cgcccggcct ggatgaagcc tgagttcatc tggaagagcg cggggccgtg gttgttgctc
     4201 tccgcatgca tggagcggat cacgcacaag ctgtcggcat ggcgcgcgag attggggtac
     4261 acctccgcca tgtcaatgcc ggcctgcccg tgtctcgcga agcggtaggg acttcgcatc
     4321 gccacgtttt tggtcttgcc gcccatgaag gcgtcctcct ttttccagac cgggatggct
     4381 ttgccgtgcc actttgccag cgcgggtttg ggatcgaaca aatccacatg ggagggcccg
     4441 ccatacatga aaagaaagat caccgacttc gccttcggcg tgaaatgcgg cgcgcgctgc
     4501 gacaaggggt ttgcgccgtg cagcagcccg tcacggccca gcagcgactg caaggccagc
     4561 gagccgaagc cggcgccggc tttttgcaaa atgtggcggc gcgtggtgaa tgaggtcatg
     4621 gaatatagac aaactcactg ctgttgagca gcacatgcgc gaggtcccgc aacgcctcct
     4681 ccgtgccggc ggaaagcgcg cgctcgccga taaaagccag cgcctgctcc atgcgcgccg
     4741 cagtgggtgc gcgcgagagc gcgagcatga acagcctctc gatgacacgc ggcaggtctt
     4801 cgcgcgctcc ggcggcggcg cgccgcgcga gatggccggc ctgctcctcg acgaactcgt
     4861 cgttcatcag caccagcgcc tgcgtgggca cggtactgcg ctggcgcatg gcgcaactgt
     4921 ccgtggttgt cggcgcgtcg aacagctcca tgctgggcat cagcaactgc cgtttcacat
     4981 aaatgtaaac gctgcgccgc cattcgccgg ggccttcctg cgcgagcacg ggccatttgt
     5041 tgcgctggct ggcgtcgagc aggtcggcac ggatgcgtgg tttgatgccc ggaccgccca
     5101 tcacggggtt cagattgccg ctggcagcca ggatgccgtc ccggatgacc tctgcctcca
     5161 tgcgccgcag gttcatgcgc cagagcagcg tgttggccgg gtccgccgcc gctcctcgcg
     5221 cgtggctgga gctttgctgc cggtaggcgg cggacatgag catgagtttg tgcagcggct
     5281 tcaaacgccc gcctccggcc atgaagcgcg cggcgagcca gtcgagcaag tcgggatgcg
     5341 aaggcgccgc gccggagagg ccgaagttgc tcggcgtggc gaccaggccc gcgccgaagt
     5401 gatgctgcca cacgcggttg accatcacac gccaggtgag cggattttcc ggggaggcga
     5461 tccactccgc cagcgtcccg cgccgcatgg aggtcctgcc attgggccgt tctgggttca
     5521 aagaccgccc tccaagcaca gcgggcgccg ccggcaaaac ctcgtccccc atgctggcgg
     5581 ccaggccgcg cagcatcaca tgagtgggcg gcgcgtcggg tttggtctca atgagcgcca
     5641 tgacccgcgc gtcgtcactc accgccttgt ccctggcatc cgtggcacct ttgggaaggc
     5701 cgagacgctc ggtcccggcg aagacggcat ggaggctgta atagtccctg gcgctcagcg
     5761 gctcggtttt gtggtcgtgg cagcgcgcgc agccaagcgt gaggcccaga aacacggaag
     5821 ccgtggtgga gatgacatca tccatctggt ccgcgcggta ttgcgccagc gcctccggcg
     5881 tcttgcccat gttgtcatcg ttgctcggac cattgcggca aaagccggtg gcgatgagcg
     5941 aggtctcgtc cgctccctcg atttcatcac cggcgatttg ctcggcgatg aagcgcgcgt
     6001 agggcttgtc ctcgttgaag ctgcggatga cgtaatcgcg gtatttccat gcgtggggcc
     6061 ggtccaggtc gttgtgaaag ccgctcgtgt ccgcgtagcg cgccaagtcc agccagtggc
     6121 gtccccagcg ctcgccatgg gcgggacttt cgagcaggcg gtcaatgagc cgctcatagg
     6181 catgaagcga gtcgtcatta acaaacgcct ccacctcatc gggcgagggc ggcaggccgg
     6241 tcagatcgag cgtcacacgg cggatcaatg ccgcgcggtc cgcctccggc gagggctgca
     6301 atcccgcagc ctcaagtttg gcgaggatga agcggtcgat gtcatttctc agccatccag
     6361 cctgcgccac ctccggcggc cgcggttgcg acagcggctg aaaggcccaa tgcgcgcgcc
     6421 cttcctccat gccaggcggt tttgcctgcg cggtcaggat gcatagggac agcagatgga
     6481 gaggggagac tttcaaccct gtcttcaacg tgaatgccat cgcccattgt tcgcgaactt
     6541 catcccatcc aatcaacccg gcgccggagg ggccgatctc cgggctggca agacgacgca
     6601 tctgctgtaa aacgggacct catgcgcgct gtcattctgt gcttgtgtct cgccttccac
     6661 gcttctctgc aggcgcggat gaatgtgctt ttcatcgcca tagacgatct ggggaacgcg
     6721 ctgggctgcg acggcgcggc gggggcgcgc acgccgcaca tggacctgct ggcggcctcg
     6781 ggagtgcgtt ttgaccgcgc ttacaaccag atgccgttgt gcaatcccgc gcgcgcgtcc
     6841 ctgctcaccg gacgcaggcc ggatgccgct caagtttatg atctggaccg gcatttccgc
     6901 gaggctttgc cggatgtggt gacgctgccg caactgttcc ggcaaaacgg ctggctgagc
     6961 gcgcgcgccg gcaaaatcta ccactacgat gtgcccaaag gcatcggcac cgacggcctg
     7021 gatgacaggc cctcatggga tgtcgtggtc aatcccaagg ggcgcgacgc gcatgacgag
     7081 gcgctcatca tcaatcccac tccggagaag ccggtcagcg cggcgctgag ctggctggcc
     7141 gccgacgggg cggacgagga gcagacggat ggcatgatcg ccacggaggc aatcgcgctg
     7201 atggaaaaac acgcgggccg cccgttcttc ctcagtgtgg ggttcttccg tccgcacacg
     7261 ccgtttgtcg cgccgaaaaa gtatttcgaa atgcatcccc tggagtccat ccgcctgccc
     7321 gatgcgccgg cggatgaccg ggcggacatc ccggtggcgg cattcgcgca caacaatccc
     7381 gtgcctgact atggcctgga cgagctggcc tgccgcagag ctttgcaggc ttaccgcgcc
     7441 agcgtgtcgt ttgtggacgc ccaggtgggg cgcgtgctcg acgcgctgga gcggctgggg
     7501 ctggcggaga ataccatcgt cgtcctgtgg agcgaccacg ggtaccattt gggcgagcat
     7561 ctgggcgtgt ggcaaaagcg cacgctgttc gaggagggcg cgcgcgcgcc gctgctcatc
     7621 cgcgcgccgg gccgcgcggg caacggacgt gtgtgcgggc gcgtcgtcga gttcgtggac
     7681 atctatccca cgctggcgga tctggccgga ctcaagccgg cgacgccttt ggacgggcgc
     7741 agcctggtgc cgctgcttga tgatccagcg cgcgcctggg atcatggcgc tttcacccag
     7801 attctacggc ccgcggatga ccgcctgccc gcgcaggtga tgggccgcac ggtgcgcacg
     7861 gagcgctggc gctacacgga gtggaacggc ggcaaggagg gcctggagct ttacgatcac
     7921 ggggatgatc cgcgggagtt tcgcaaccgc gccctgaacc ccgacgccga agccgtggcg
     7981 gcgatggggc gactgcgcgc actcttcgaa gggcgcgccc gcgccttgcc cccggattcg
     8041 ccggtgaatg cgaaaaggct gtgacccccc cgcgccggcg cggtcatgcg ccgcttgccg
     8101 atcctccaac cccgcctcca gtcaacgcgc atgtccgccg aatcccccgc cacgctcaaa
     8161 gacatcgccc gcgcggcggg agtgtccatg atgacggtgt cgcgcgtgct gcgcggagcg
     8221 cccagggttt ccgcggaaaa gcgcgagctg gtgctgggcg aggcggagcg gctgaactac
     8281 cgtcccgatc cgcatctggc gcggatgatg caggcggtgc gggcaaggaa gcagaagcgc
     8341 gtgcgcgcgg tgatcgcggt catccgcgag catgtgccgc aggacggcct gctgagtccc
     8401 gcctatcaat atgttcaggt ggaggacatc cgccggcgcg ctcacgggca tggctacgcg
     8461 gtggaggagt tttttctcgg cagggacggt ctgacgccga agcgactgca aaagatcctg
     8521 cacgcgcgcg gcatcgaggc ggtgatcgtc tcgccgcaga gcacgcagct tccgtgctcg
     8581 cgcctggatt acgcgccctt cgcggcggtg acttttggtt atgcgatgag ttcgcccgcg
     8641 ctgcacatgt gcgcgggaaa catgacgctg ggcatccaga cggcggcgcg ggagctggct
     8701 cgtcggggct gccgccggat cggtgtggca gtgacgaaat ggatcgtgaa ccgctcgcag
     8761 ttcggctaca gcggcggtct ttttcactgg cagcaggacc tgtatccgga gcagcgcgtg
     8821 ccgctgctgc tgtttccaga caatgacatc agccggggct tcgaaccctt cgccgaatgg
     8881 atgcgccggc acaggccgga cgcggtgatc tctttcgacg cgcatgtgcc cggctggctg
     8941 aaaagactcg gtctgcgcat tccgggggac atctgctttg tggcgcatga ctggacgccg
     9001 aggatgcgcg gtttcgccgg catctaccag cagcgggatc atctggcggc ggccgcggtg
     9061 gacctgatcg tcacgcagct ctcccagcac gagcacggcg tgcccgaggt gccgcgtcag
     9121 atcatgatcc cgccgcgctg ggtggacggg ccgagcgtgc gtgcgctccc gttggcgtga
     9181 agccggcgac gtctctgcgg atcagcgcca ctttccaagg cgcggtgttt tctcccgcag
     9241 ccatcccatg gtcgcgaaaa atggggaagc ggcgtcaggc aactgcctga caaaagaagc
     9301 gcgttcatga actcgccgca tgggctgcgc aaaagtattt cagggtttgc cgtgctttct
     9361 tcatttcaaa tgtaccgtca agagttctcc agtgttggct tgcagatcag tccgagcgct
     9421 cgcacgcctg ggagtggggg cggagaaaaa agcgccagtt ttagcatctg cgccaatacc
     9481 gcaaggaatg gaccgcttct ttgaagaaaa cgcgtcaaga tgaaagtagc tggagccttg
     9541 attctacggg gcctccaacg tgaagcgaag tgttgctaaa ggcatcgtca ggcgttgttt
     9601 ctgaacacgg ttctgccgcg cttttcgtgc attggcacgc ggcgtgtctc ttatgcggcc
     9661 tgcttttgtg tgaagctggg tggtagctcg cgtcatcagg atttgtgatt cttttcctgg
     9721 cttgatgaag tttagaagtc atggcgtttc catgctccgg cactccatga gtccagcgga
     9781 ctggatttgg cttttatggc gatgccgggt ttgtttatct tctgtcaaca cgccagtttc
     9841 agcctcatgc gcctgctttt ttcctgcctc gtcctttttc acgccaccgc tttcagctcc
     9901 gacttccccg cgccctacaa cagcgagcct ggcaatccct cgccgatgtc gccgcaggag
     9961 gcgttgaagg cgctgaagct gcccgagggc ttcaaagcca cgctctttgc ggccgagccg
    10021 gacgtgcaga atcccatcgc gatggcttgg gatgcgaaag gccgcatgtg ggtggcggaa
    10081 aactacacct atgccgagcg cgcgaagcgc ttcgacctca cgctgaagga tcgcgtcatc
    10141 attttggagg acaaggactg ggatggcatc gccgagacgc gcaaagtgtt cactgaagac
    10201 gtgcagatgc tcaccagcgt cgaggtcggc cgcggtggcg tgtggctgat gtgcccgcca
    10261 caggtgctat tcatcccgga tgcgaatggc gacgacatcc ccgacggccc gccgcaggtg
    10321 gtgctggacg gcttcaccgt ggcggaggca aactaccaca acttcgccaa cggcctgcgc
    10381 tttggcccgg atggctggct ctacggacgc tgcggccact cctgccccgg caacctcggc
    10441 gttcccggca cacccgatga caaacgcgtg ccgatgaaag gcggcatctg gcgcttccat
    10501 cccgaaaaga aaatcgtcga agtgctcacg cacggcacca cgaatccgtg gggccacgat
    10561 tgggatgcga acggcgagat gttcttcatc aacaccgtca ccggccacct ctggcacctc
    10621 atccacggcg cgcatctggt ggactcgaac acgccaaacc cgctcatcta tgaaaagctc
    10681 gacaccatcg ccgaccacta ccactacgac cgcaccggcg cctggagcga ctcgcgcgac
    10741 ggcaaggcca accacctcgg cggcggccac gcccacatcg gcatgatgat ctacaaggcc
    10801 gaccaatggc cggagcaatt ccgcaacaag ctcttcaccc tcaacatgca tggccgccgc
    10861 gccaatgtgg agcgtctgga gcgcagcagc agcggctatg tcggcaaaca cgagccggat
    10921 gtcttcctca gcgacgatcc ctggttccgc ggcatcgaga tcagcaccgg tcccgacggc
    10981 agcggcttca tcctcgactg gagcgacact ggcgaatgcc acgaatccac cggcgtccac
    11041 cgcaccagcg gccgcatttt tcgcatcagc tatggggacc cgaagatgcc ggagaagccc
    11101 tttgcgtggc ggaaaccttg gccgaaggca gaattgaaat cacccgatga atacgcgcga
    11161 gtgcttgcga ttcgagaact gacccaattc atgccgttgg acacgattat cggtccgatg
    11221 cctggatccg attatttcaa gcatgtgccg acagatgtgc tgaaactgct cgaaatgacc
    11281 gctgtgaatg acaagtcgag cctcgtccgc ctcactcttg cctcgaccct gcaacggctg
    11341 ccagtttcgc ggagacaaaa tttggcggca atcttgctct ctcacaccga ggacgccaac
    11401 gacccgtttt tgccacacat gatctggttc ggcatctccc ccctgaaggc tgaggcgctg
    11461 ctcgaaatcg cccactcctg ctcgtggccg aaagtgaacc gctggatcgc ccgcaagctc
    11521 gcgaccgatc cggaggcttt gaaccaactg ctcgccatca acagctcaga cgacgtgctc
    11581 gaaggcatgg ccgaggcctt ccgcggcatc cgcaaggcgc cgaaacccgc gaactgggac
    11641 aaagtcgttt ccacctccaa atccccctat gtccgcgacc tcagcatcct cttcggagat
    11701 ggccgcgcgc tggatgatgt gaagaagctc gcactcgatg ccaaggccga tcccgccgcg
    11761 cgcgaggccg cgctcaggac gctcatcgag gcccgccccg atgatttgcg cgaggtttgc
    11821 acgaagctga tcgaggtgcg cggcctcaac accaccgcgg cgcggggact ggcgctcttc
    11881 aatgatcccg agatcggcag acagctcgcg aagaactacc gcaaattccc cggcggcatc
    11941 atggacacgc tcgtctcacg ccccgccttc gcacaggcca tgctggccga gatggccgcc
    12001 gggcgcatcc cgcgcaccga gctgagcgcc tttcacgccc gccagatccg ctccttcaag
    12061 gacgagtctc tttccaaaca gctcgtcgag gcctggggcg agctgcgcga gtccgctgcc
    12121 gataaaaaac agctcatcga aaagctgaag gctgacctga agccggaggt gctcgccaag
    12181 gccagccttg gccaggggcg cgtgctttac tccgccctct gcggcgcctg ccacatgctg
    12241 tatggcacgg gcggcaagat cggccccgac ctcaccggca gcggacgctc caacctggat
    12301 tacctgctgg aaaacatcgc cgacccgagc gcggtcgtca gtgcggacta ccgcatgagc
    12361 gtcctcacgc tcaaggatgg ccgcatcctc agcggcgtca tcgcggaaaa aaacgagcgc
    12421 accgtgacgc tgcgcaccct gaccgaatcc ctgacgctgg accgcgccga gatcacgaaa
    12481 acggaaacct cccccatgtc catgatgccg gagggcctgc tgctggcgct gcaggccgat
    12541 caggtgcgcg atttgatcgc ctacctcatg cacccggtgc aggtgccgct gccagacggg
    12601 cgctgaaggg acggttggca ggggggggat cgcatggacc ggcgccccat cccttatcat
    12661 ttcaaaaaga ccggggcgta tttcaccgtc cagtcttcgg ggaggcggag gggccggcct
    12721 tgcggcatct ggacgagagc gagagactgg cggcaggtga tgagcacggc gtcctcgccg
    12781 gcgcggcgga tttcgaaggc cacccagaag cgcacgcgct ccagtcggtc gagtctgccg
    12841 gtgatgacga gttcgtcgcc gagggtggca ggcttgcggt agtcaatttc ggtgcggacg
    12901 acgacgggat agacctgggt gcgcgccatg tcgcgcagct tcatgccaag tctggccgcg
    12961 aggcgtgtgc gcgcggtttc gatcatgcgc aggtaggcga tgttgtggac gacgccgccg
    13021 atgtcggtgt cgaaaaacat gacctcctcg cgggtctcga tggtggggat ttcttctgcg
    13081 ctcatgacgg gtttccctgg cctgaaaggc gcgcgctggc aaatgcgcag gcgcattttt
    13141 cagcggctcc tgccgaggac gacgcggaag ccgagcgctg ggctgcggtg gtccgccggg
    13201 tgggacgcgc gcgctgaggc gagcagctcg tcggcgctgg cgctgcgcca gttcccgccg
    13261 cgcgcggtgg cgagcacggc cttgctgccg gcggcggctt tcctgccgtt gtcgaagtcg
    13321 gtgtccaccc attcggagac attccctgcc agcccgagaa tgccgcgctc gttggcgggg
    13381 aaggcgccca cgggcgagag ctgggggaag ccgtcctcgt agccggggat gatgttttcc
    13441 aggttgcctt tccgcatggc gccgatgtcg gcgaagttgt cggttttcgg cggcggcggc
    13501 cagtcaaaac cccaggggta gatgccgcga atgcgcccgt tgcgctctgc gggggtgact
    13561 ccgcgctcca gcggcagacc gacggcgcgg ctccactcct cgtcggtggg cagacggtag
    13621 gtgtcggcgg ggccgatgag gccggagttg cgctcgcgct cggtgagcca ggcgcaaaag
    13681 gcgcgcgcct cctcgcggtc aacgctgacg acgggctggg tggcgccgcg gccgtcggcg
    13741 tccaggtttc cgggccggcg gcgattggcg gctttcgcga attccaggta gtcgcgccgg
    13801 cgcgtctcgg tggcggcgat catgacggac tcgcccagcg gaacgaggcg catgccgagg
    13861 ctgttggcgg tccactcgcg tccgaaggtg acggcctgtc gcgtctgcat gtcggcgaag
    13921 agctccaaaa gctctccagg gcgcacctcg ccctccagga tgacatcgga gaagccctcc
    13981 tcccgcaggt cgtactcggc gctgccgctg cgcacgcgcg gcagctccag cggtgtgtag
    14041 ccaagaaatt gctcatgctg aaacacgcgc actttttccg gcatggtgcg gatgacgacg
    14101 ctgccgtagg actggcgctc gacgcgcaaa ttgaaggcct gccagtcctg gctctcctgg
    14161 cttgcgccct cggtggagtc gcggtcatcg ggcatgtcct cgctgctggc gcctgtctcg
    14221 acataataaa agggttcgac ctggtattga tgctcctggc tgaggtgccc ggcgcggcgg
    14281 tcctcctcgg tgagccagaa gcggaaggcc tcggcgtcgc cgatcggtgc gacgatgaag
    14341 tacgcgctct ccttgttctg gccggcgcgc acgacgcggc cttcaaagga gcggccggtg
    14401 tcgtcgagga agcgtttgaa atgcttgatc tgcacgggtt cggtggtggt gtggccgttg
    14461 agcaccggct tgaatgtcat gccgaggctg ttggtccagc gctcgcccgg ctggggcagg
    14521 cgtgtggcct ccatcttcac gtcaaagcgc gcgggctggg cgcgggtggc gatgtgctcc
    14581 agctccatct gcttgtggcc cgtcatgcgg agctggtaga tgacggagac gccctcctcg
    14641 gggttgatct ccaggggcgt gacgccgagc aatttctccc ctgcaaagac ctccgcaccc
    14701 ggcggctcgg tgctgatggt gagcagcggc gtggagctct gcacggccac cagggcgccg
    14761 ccttcgcgca tagccagcca catgccaaag ccgaccgcga gcgccagggt gacgacggcg
    14821 gccgccgccc actgccacac agcgcggcgg gagggcaggg gctggccgcg caggtcgaga
    14881 gccatttccc aagcgctgga gtagcggtcc ttggcgcgcg gggcgcaggc gcggcagatg
    14941 accttgtgca gacgctgcca gtcctcgagc tggtcgcccg tctcggtgca ggaggggatg
    15001 tcggggaaat ccaggcggtc tttgccggtg ctggcctcat agaggaccat gccgagggaa
    15061 aagatgtccg actccggcga ccctggcccc tccggcgcca cgaagccctc cgtgccgacg
    15121 aagctgcgct gccccatgag cgccacgagg ccgatgtccg ccagccggca ctgcccgtct
    15181 atgaagatga ggttggaggg cttcacatcc ctgtgaatga gcttgtgctg gtgcaggtgg
    15241 tgcagcccct cggccacatt cgcgccaagt ttcaggcagt cccccgcctt gagccgtttc
    15301 tgcctgcgca tttgcaggcc gaggatgtgc ggcttgtagg tctccgcctt gatgtcgcgc
    15361 ccgcgctcca ggtcgtcggc gagctccatc acataataat aaaagccctg ctcgtcactg
    15421 cgtccgacct ggaggatggg cacaaggccg ggatgcttgc gtgagacggg atcgtaatgc
    15481 ttgatggcct cgaactcgcg ctcgaaggag tccgcgtggt catagtcctc ccgccacacg
    15541 accttcaccg cgcgcaacgc gccggtgacg ctgcgcgcca tccacacttc cccatacgcc
    15601 ccgcgcccga tcatgcgcag ggtctcatgg tcgcggattt ccggcggtgt gcggctgggg
    15661 tcggacattg gcgcggatta tccggcagtc tgacgcgcgg ttcaagacgg gaaaatgagg
    15721 gcgttgtggg atccaggcca gcccagaggc gatgggcaat gggcaatcgg tccgattttt
    15781 gatttttcaa gtttctcccc cgctcaacgc gcgccattcg tcccctccgt ccatgcccat
    15841 ctccgacgac ctccgcgatc tctaccagca agtcatcctc gaccactcgc gccatccgcg
    15901 caattacggc gagctagccg gggactgcgc tcacgcgcat ggcgacaacc cctcctgcgg
    15961 cgacgaggtg gacctgtggg tgcgcttttc tccctatggc agcattgagg acatcaagtt
    16021 cagcggccag ggctgcgcca tcagccaggc cagcgcctcc atgatgaccg tgaaggtcaa
    16081 gggtttgacc aaagagaagg cggcggccat gatcgaggac ttccgccatg tcgtcacggg
    16141 cgagggcgcg gtgcgtgacg aggacgccct gggcgagctg caactgctcg aaggcgtgca
    16201 aaaattcccc cagcgcgtga agtgcgccat gctggcctgg cgggcgctgg agcaggcgct
    16261 ggcgggcgcg cgggtcaatc cggaatgggg tcgccacgtt tgacgggggc gccttcccag
    16321 atggcggtgt ccgtttcctt gtcgtaggtg agcacgcggc tggcgctgtt cggagcgggg
    16381 ggcaggtcca ccttgggcag ccattttttg tgcccggcga tgacggcggc gtgctcgggt
    16441 ttgcctgcga gattatgcca ttcgcgaggg tcggcctgca tgtcgtagag ctcctcgctg
    16501 ccgtcggcgt attggatgta gcgccagcgc tcgctgcgga tgccgtggtt gccctggttg
    16561 tggctggtga tggcgggacg ttcgcgcttg gtggcggcgt ccttgagctg gggagcgagg
    16621 ctgaggccct ccaggtcgtc gcgcgcgggg acgccggcga gttcgacgag cgttgggtaa
    16681 atgtcgagca actcggcggg ctgggtgcag cgctggccgg cggtgacgcc ggggccggcg
    16741 aagatgagcg ggactttggt gccgcggtcc cagagggtgt ttttgccggt gatggctttt
    16801 tcccccagat gccagccgtg gtcgccccag acgacgacga tggtgttgtc ggcgatgccg
    16861 gcctcgtcga gcgcgccgag caggcggccg atctgcgcgt ccacgaagct ggtgcatgcc
    16921 aggtagctgc gcacgaggtt gcgccattgg ttgttttcct tcacccactt gaggcgcggc
    16981 tcgggcaggt tccagtggat gtaccaggaa aagcggggcg tgtcgtcacg gtcgccctcc
    17041 agggtcttcg gcagcacgct gtcgtcgtcg ggataaagat cgaaccactt ctgcgtggcg
    17101 tagcagggga catggggtag aaagaacccg gcggcgagaa agaagggctg gtccttgggc
    17161 gcgtttttga tttgctcgac ggtccacgag gccacttgat agtcgccctt ctgcgtgtcg
    17221 tcattgtcca gcggccaaac tccccaatcc atcagtggat ggtcacccat cggtgttgga
    17281 gggatgagtt ttttcggcgg cttcgtgccc acgccgccat agggcgcgga gacttggaat
    17341 tcgggtggtc cctggtcgcc gcccctggcc cttgcctttg gtcccgcgcc gcgcccgccg
    17401 gtgccgccgt ggtagatttt gcccgttgcg gcagtgcggt agccgttgtt ggcaaagtgc
    17461 tgcggcaggg ccacccggtc cttccactcg ggcagggtgc ggaaccaggg ggcgagtccg
    17521 tagatgccgg tggtggtggg gcgcaggccc agcagcaggc tggtgcggga ggggttgcag
    17581 agcggggcgt tgcagtgcgc gttgaggaag gtggtgccgc gcgcggccag cttgtcgagg
    17641 ttcggtgttt tcgccagggg gtggccgccg agatggccga tccagtcgtt ttgatcatca
    17701 atggcgatga agaggacgtt gggccgtttt tcctgcgcca agagggagca ggaaaaggcc
    17761 agggcggcgg cgaggagaga tgcggcaagt ttcatgaaaa agaaccggtg gcaggcgcaa
    17821 aggaacgatg cagcggacgg cggctttcat ccgccggcgc gagcgctgtt tttgcctcgg
    17881 cgggaattcc tgctgaaagc gcccggctcc cgccgtttaa tggcgcgatg aaaatccatc
    17941 tccttctttt gccgctcttg ttttgcagcc tctccacact cactggcgct gaggaaaagc
    18001 cggtggacgc gcccgccgtg gaatccaaaa aggacgaacc cgccgacccg ctggccggac
    18061 tcaaggcgga gacagcgcgt ctcgaagccg agaggagcaa gatcaccgcg cagctcgccc
    18121 tggagcaggc gcggctggac gaaaagctgg ccgggcaaaa acgcgcggtc gcggagatcc
    18181 aggcgcagat ggaggattta aagacgcgca tggacctggc tgaaatcaag cgccgcagtg
    18241 ctgatgatcc cgagctgctg gaattgcgcc gcaagagcga gcgcctcgtg ctcgaggctg
    18301 tgatcgcgaa gaacgaggtc gaactgcaaa gtttcgagat gcgcaaaaag gagaacgagg
    18361 tgcgcgccag gacgagcgag atcacgctcc agatcgagct ccagcaaaag gaggccgagg
    18421 cgcgcactca cgccgtcggc aaggcgcccg agtatctcaa ggacccgcac cagggtggca
    18481 agctggtcat atcagaccgc cgcattgcgc tcaatggcgt catcaccgcc aagaccgcgg
    18541 accacatcgc cgaccgcatc gcctacttca acaaccgcga ctcggaggcg cccatcttca
    18601 ttgtcataga cgactgcccc ggcggcagtg tcatggcggg ctacaagatc atcaaagcca
    18661 tgcacggctc caaagcgccc gtccataccg tggtgaagtc cttcgccgcc agcatggccg
    18721 cctgcatcac caccgtctcc aaaaaatcct ttgcctatcc caacgccatc ctgcttcacc
    18781 accagctctc cgtgggcgtc gccggaaacc tcacccagca ccgggaaaat gtgaagcagc
    18841 ttgaggaatg gtggcgtcgt cttgccgacc cggtggcgga aaagatgggg ctgacccggg
    18901 aggagttcat cgcccgcatg tatcaggagg cgtccaccgg ggattggagc gagttcgccg
    18961 acaacgcgcg caaactcaag tgggtggacg agatcgtcga agacattgag gagacctccc
    19021 tgctgcgcca ccccgacagc cagcagaccg cctcgaccac cagcgccccg cgcctgcccg
    19081 gcatgccgcc gcagacggag atcatcgagt cgaacgacga gcgcggcaaa cccatcgccc
    19141 tgctgccaag actgaatccc ctcgacgcct actggcttta caatccggat ggtttctacc
    19201 ggttgcagtg acgcgcgtcc ggcatgggga gcatgagatg tggaagcgcc ggttttccag
    19261 gtcgggtgag ctactcccgc atcttgtccg caaacaagtc cacagccgtg acaatcgagc
    19321 tcaacatgcg cttgaaaacg tcttcaaaat cctccgggat ggcgacgaat gactccgctg
    19381 tgacccagac gttgtcgccc ttggtgtaaa tcttggccat tttggtctcc tgatttgtct
    19441 catcagcggc cgcgagaacc ttcctgcgct ccacggagct ctcaatcggc cagatgttgg
    19501 gaagggcgat catgaagaac tccgagtcct tatcgtgaac atcaatgaag taggttttgc
    19561 cttcgtgttt gaaaaccacg tcgccatcac catccacttc gggttgataa ccctcttttt
    19621 tcaagaactt ggtgtacatt gccctcagtt cttcgccgct tttggcatcc acttcgagcg
    19681 gaagcacggc ttgcatgaag atcaaagcgg cgagaacccg ggaggatgag atgaaaagat
    19741 tcatagcacc gccacgcttg tccatgcagg tgcggggtca aagggaaatc tgaaaaactt
    19801 gcgtgtgccg agggcattta tctgttccag cgagacaagg catcgtagcg cgccttcgca
    19861 gggcgcggca tattcccgca agatcaatga accgggatgg gttatcagcg atcatgccca
    19921 agctcctgcc gcgtctcatt ctttacacct gcgtctttgc cgcattcagt caggtcaagt
    19981 ttcccgccgc accgccgaat gtggtgattc tgtttgccga tgatcttgga tacggcgatc
    20041 tcggctgcca gggatcgccg gtgatccgca ccccgaacct ggaccgcatg gctgcggagg
    20101 ggctgagatt cacggatttt tactccgcgg cggaggtttg cacaccgagc cgcgcggcgc
    20161 tcctgaccgg gcgttacccc atccgcagcg gcatgtgcga gacaccgggg gcgcgcaggg
    20221 tattgtttgc gaactccaag ggcgggctgc cgcagtcgga ggtgacactg gcgcgcgcgc
    20281 tcaaggcgcg cggctatgcc acggcgcatg tgggcaagtg gcacctcggc attcatgagg
    20341 gcggacgtcc gctggagcac gggttcgaca gcagcttcgg cctgccatac tcgaatgaca
    20401 tggacgggcg ccccggcctg ccgaaaggct ccagcggctc gccgaatcca ccgcaggacg
    20461 gatggaatgt ggcgctgatg cgcgatggca aagtggtcga gcagccggcg gaccagacga
    20521 cgctgacccg ccgctacacg gaggaggcgg tcaaattcat cacggagaag aagggcggcc
    20581 cctttttcct ctatctggcg cacaccttcc cgcatgtgcc catgttcgcc tcaccgcaat
    20641 tcaaaggcag gagcagggcg ggcatttacg gggacgcggt cgaggaactt gactggagca
    20701 cgggacaagt cctcgacgca ttgagaaaag aggggctggc ggggaacacg ctggtctttt
    20761 ttaccagcga caacggcccc tggctcatca tgggcagcca gggcggcagc gcgggtctgc
    20821 tcaaagacgg gaagggcagc acctgggagg gcgggatgcg cgtgccgggc atcgcgtgga
    20881 tgcccgggcg cgtccagccg ggcgtgacca gcgctgtggc gcacatgatg gacttgtttc
    20941 ccaccgcgct ggctctggcg ggcgtggaaa agcccgctgg tgtcaccctc gatggtgttg
    21001 atctggctcc catgctcttt gccgggcgtt cattgccgga gcggcccttc ttctattacc
    21061 gcggcgatca aatcttcgcc tgccggctgg gggaatggaa agcgcacttc aagacgcaga
    21121 ccggctatgg ccagccgaaa gcggaggcgc atgagaggcc gctgctgttc cacctggggc
    21181 tggacccttc ggaaaagcgg gacatggctg cgactcatcc cgatgtcatc gcccgcatcc
    21241 aggacgctgt gcggacgcat caggcggcca tgacaccggg cgcgccgcag ttgcagtgag
    21301 gccccccggg cgccgcgtgg cgaggcgccg ggatagcgtc accaattctt gatccacttc
    21361 tcgctggcct gcctgagagg gcctcctgca ttggattcga tggatgtgga catcgtgttt
    21421 tcctcgtcct cgccatagga ccagagatca taatgggtgt tgatggtggt cgggttcgcg
    21481 cccgcgcccc cggtgaggtt ctggggtagc gctttgacgt actggatggg cttgctgaag
    21541 ccgtctatga ggaaaaagcg gccttccagt tcgcgccaga tgtccttggg catgtcggtg
    21601 atcatcgtgt ttctcgactc gtcagcttcg acctggccgt tggactgggg attgccggcg
    21661 ttggcggggg gcacggctat tttgatggcg tcatagccgt cgccgctcat ggcctggtag
    21721 agcatggacg cgccggagac ggtgtaggtt ttgtcctgga tggagatcgt gtcaccaggg
    21781 ctggccggtt cggggtactc tccgaactcg gtgttgtagc gctccaggcc gctctgcacg
    21841 gccttgatgg tggcggcggt cttgctgcgg ttggcaaagc gctgcgcata ggtgaaaccg
    21901 ccgatggtga ggccaaaaag cagcgcgatg atggtgatga cgatgagcaa ctcgatcagg
    21961 gtgaagcctg cccggctgtg tttcagcatg ggggttttca tggttggcgg gggtggcggt
    22021 tttttgaaaa cgctccggga tcagcccccg gcgctgaggc ccttgatgac ctcgatgagc
    22081 gggaggaaaa gcgccatgac gatgacaccg accacgagcg cgagcagcac gatcatgagc
    22141 ggttcgagca tggaggttag ggcggagacg gagttgtcca cctcgtcctc gtaaacatcc
    22201 gcgatcttga gcagcatctc ggggagctgg ccggtttcct cgcccacgtc caccatggag
    22261 atgaccatgg gcgggaagac attgctggcc tccagcgggc cgaccatgga ctcgccctct
    22321 ttgacggcgt cgtgaacctt ggtgatggct tcggagacaa cggtgttgcc ggcggtctcg
    22381 cgggtgatgt tcagggcctg gaggatgggc acaccagagg tgacgagtgt gccgagggtg
    22441 cgagtaaagc gggagatggc ggtcttgcgc tgcacatcac cgaagagcgg cagcttgagc
    22501 ttcattgagt caatggagcg ccttccgccg ggggtggagg cgaacatttt ccagacgacg
    22561 acaacgacca caatgccgag gccgagccag agcacattgt cacccatcca ccggctgaac
    22621 ccgatgaccc acttggtgag ttcgggcagc ttgtttttat ctcccagcat gtcctcgaag
    22681 attttttcga agcgagggac gatgaccagc ataaggaaga ccatgatggc cagggcgatg
    22741 aacatgacga tgatggggta caccatcgct gcgacgacct tgttcttgag cttctgggcc
    22801 ttttcctggt attcggcgag gcggttgagc acgatttcga gcacgccgcc gagctcgccg
    22861 gccttgacca tgttgatgta gagcttgttg aagattttcg ggtactgcga gagcgtctcg
    22921 gagaaggtgc tgccggtctg cacgttgtcg gcgatggtgc tgaccgtgct cttcatgacg
    22981 gggttcggct cctggcggct gagcacagtc aggccgcgca gcaggggcag gccggagtca
    23041 atgagcgtgg cgagctggcg ggtgaagatc atcaaggtct tgggcttgac cttggcattg
    23101 gctccggctt tgacggcggt cttgcccttg gatttggcgg cggtggtttt ggcggctttt
    23161 ttgaccgccg cgtctcctct gccctcctgg gccacctggg tggggtagag ctggctttgg
    23221 cgcaggaggt tgacggcttc agcctcgttg gcggcgtcga gttcgcctgt gacttcttga
    23281 ccgttttgat cgagggcgat gtataggaac ttcggcataa aggggatggc ggagcggaat
    23341 tctaaaacaa atgcagatta ccggggtgtg gcggaaaatg tccaataaat aaatggtgtc
    23401 ggaactgaca aaattggcgg gaggcgcgcg cgtcaggtgt atttgagcac ctcctcaatg
    23461 gtggtctcgc cctcgtagat gttgcgcagg ccgtcttcgc gcagggtctg catgccaagt
    23521 tcgatggcct tttgcttgag cacgagggtg ggggccttct gggtgatgag ttcgcggatg
    23581 ggatccgtca cgtcgagcag ctcatagatg cctttgcggc ccttgtatcc gctgccgccg
    23641 cacttgtcgc agccgttgcc ggtgtagaag tgcttgccgc cgatgtcgtc ggcggagagg
    23701 ttgagctggt tgaggatgga ctggctcggc tcgtaggggg cgcggcagtt tttgcagatg
    23761 gtgcgcagca ggcgctgggc gagcacgccc tcaagagtgg cggcgacgag gaaaggctcc
    23821 acgcccatgt ccacgaggcg ggtgatggcg ccggcggcgt cgttggtgtg cagggtgctg
    23881 agcacaaggt gcccggtgag ggaggcctgg atggcgatct gcgcggtctc cttgtcgcgc
    23941 atctcgccga ccatgatgcg gtcgggatcc tgtcggagga aggcgcgcag ggcgcgggcg
    24001 aaggtgaggc cgacggcgtc gttgaccggc acctgcatga tgccgtcgag ctcgtattcc
    24061 acggggtcct cggcggtgag cagcttgctg tccacggtgt tgatcttgcg caggcaggcg
    24121 tagagggtgg tggtcttgcc cgcgccggtg gggccggtga cgatgaagat gccgttgggc
    24181 ttttcgatgg tgtcctcaat gtagtcgcgc aaaggcccgg gcatgccgag gtcggtgagc
    24241 tcgagctgga tggaggagcg gtcaagcacg cgcagcacca cggactcgcc gtgctgggtg
    24301 ggcagggagc tgacgcgcat gtccaccgcc ttgccgttga ccaccgtcat gatgcgcccg
    24361 tcctggggga tgcggcgctc ggcgatgttc atgttggaca tgaccttgat gcgggagatg
    24421 acgctggtgg ccaggtggac gggcggcggg gccatctcgt agagcgcgcc gtccacgcgg
    24481 tagcggattt tgaattcgtg ctcgaagggc tcgaagtgga tgtcggaggc tcgctccttg
    24541 atggcctggg tcatgaccag gtccacgaac ttgacaatgg gcgcggagtt ggcctcaact
    24601 tcgggggcgt ttttgccggc gtccttgagg ccgccgaaga tctcgtcaat gttcggagcg
    24661 ctggtgccgt agtgcttgtc tatgagatcc tgcacctggc tgcggcgggc cacgacgggg
    24721 acgatgatct tgtcgagacc gaagcgcagg tcctcgacga gctgggggtt tagcgggtcg
    24781 gtgaaggcca catgcaggcc gcgcccgtcg agggtgacgg ggaaggcgcc gtaaaggcgg
    24841 gccatgccgc cggggatgag gttgaccacg gagggggcgg gctcgaagga ggcgaggtcg
    24901 aagtgctccg cgcccagctc ctcggcgacg agcgaccaga agacatcctc gttggggaag
    24961 atctcatagt cgagcagggc ctggaggatg tccttgccgt tctgcaccac atcctgcgtc
    25021 aaatcataag cctggccgct gtctatcact ccgcgtgcct ccagcatgtc caccacgttt
    25081 tgggctgtca tagcgtctgt ctttgtcttg ggttctcggg ttcagtgttc tgcggcggct
    25141 cattcggcgt cggccatggt ggcctcaatg acctcgcccg ccgtggtcat gccggccatg
    25201 accttgcgga tgccgtcctc gcgcagggtt ctcatgccca gctcgcgggc gcgcttgcgc
    25261 agttgcgggg tggtgagctg catgttgacc atgttgcgca cctcgtcgtc caccttgaag
    25321 atttcggcga tgaccatgcg gcctttgaag ccggccccgc ggcacttgtt gcagcctttg
    25381 ggtcccatga tgttcgcgct gtaaagctgg ctggcctcca ggcccaggtg gcgcatctcg
    25441 cgctcgttga ggctggtcgg ctccttgcag gttccgcaca gcttgcgcac caggcgctgg
    25501 gccaggatgc cgcgcacggc ggaggcgatg aggaagggct tgacgccgat gtcggcgagg
    25561 cgggcgatgg agctgggcgc gtcgttggtg tgcagggtgg agaagaccag gtgccctgtg
    25621 agggaggcgt tgatggcgat ggaggcggtc tccaggtcgc gaatctcacc gatcatgatg
    25681 atgtttggcg cctggcgcag gatggctttg agggcggcgc cgaaggtcat gcccacatcc
    25741 tccttgacca tgacctggtt gatgccggcc agctcgtatt ccacggggtc ctccacggtg
    25801 atgatcttgc ggtcgggccg gttgatgaag ttgaggcagg cgtaaagcgt ggtggtcttc
    25861 cccgacccgg tcggcccggt gaccagcacg atgccgtcgg gcagggtgat gagtttttca
    25921 aaagtgtcct ggtcgtcgga gaggaagccg aggtcggaga gtccgaggcg caggctgctc
    25981 ttgtccagca cgcgcatgac cacgctctcg ccgttgttgg tggggatgat ggagacgcgc
    26041 atgtccagct ccttttcctg gaaggccatc tggatgcgcc cgtcctgcgg cacgcgcttc
    26101 tcgtccacgc tcatggtgcc gcacatgatc ttcacgcggg cgatgatggc ggagtgcagg
    26161 cgtttggggt ggtgctccac atcgtgcagg acgccgtcaa tgcgcaggcg cacgcggatg
    26221 tccttttcca aaggttcgat gtggatgtcg gacgcgccgt ttttgaaggc ctccagcagg
    26281 atgttgttca ccagcttgat gacaggcgcg tcgtcgctct tcatgcctcc cgcggcggcg
    26341 ctggaggcgt cggtgacgaa ctcgttcccg tacacgttga gctgcagcgc gctgatcagc
    26401 gcgggcgtgg agcagaagta ttcgaggtcc ggctggatga cgtggggcag ggcgtcgagc
    26461 gtctcgaagt cataggggtc cgacacggcg atctgcatgg cagggccgtt catgcccacg
    26521 ggcacgatct tgtacttccg cgccacctcc attggcacca gggttttgag ctgcggactg
    26581 gcggcgaagc cgtgcagatc tatgaattcc atgccggagt tcaccgccac ggtctgcgcc
    26641 acctgctcgt cgctgacgac gccggttttg atcaggccct ccagggtgct ctcgttaggt
    26701 ttcttggtgg tcttgacctt ctgaaggtcg ctttccatga ccagccctgc ttcggtgagc
    26761 agttccagga gatactcttc gttcgaatac atgcgcgcgt tttgcctgat ttattgggat
    26821 gaatttcgga cactacgggg ttcccggcgc tagtgtcaat catccccgct gcaaagttgc
    26881 gagaaagttt tcttctccgg cgcgctcgga aagcgtggtc gcattctctc gacgcatgac
    26941 aaacgcgccc ttcttgccac gttcgctatc tccactccgc ccctgccatg atccgccatc
    27001 ttctttccac tctcgtcctt ctttccacgt tcgccgcccc agcccatcgc ctgggggctc
    27061 atgaggcggg cgcgcagatg gcccaggtgg ccgcggtgtt cctcgcctcg ctttcaccgg
    27121 agcagaagac gcgcgccact ttcgccttcg aggacgcgga gcgcgtcaac tggcacttca
    27181 tcccgcgcga gcgcaacggc ctgcccatga aggacatgac gccccagcag cggctgctgg
    27241 cgcacgcgct gatgaacacg ggcctgggct ttcgcggctc cgcgaaggcg gccacgatca
    27301 tgagcctgga ggaggtgctt taccagatcg agggcgcgga cgagtccaag cgcgcggcgg
    27361 tgcgcgagcg gcgcgatccg gagaagtact tcgtcagcat cttcggcacg ccggatgtga
    27421 agggcgcctg gggctggcgc atcgagggcc accacctgtc gctgaatttc accatcaaag
    27481 acggccggct cctgcgcgcc acgcccgcct tcatgggcag caaccccggc gagctgcgcc
    27541 aggggccgct gacaggattg cgcgtgctgg gcagggagga agacctgggc cgcgaactgg
    27601 tgaagtcgct taccggggag caattccaaa aagccgtggt ggcggatgtg gcgccgaagg
    27661 aaatgctcac cgccgccgag cagcgcgtgg acccgctgaa gccggacggc ctcagcgcgg
    27721 cggagatgac tgacgcgcaa aaggccaagc tcaacgagat catccaggag tatctgggcc
    27781 gcgtgcgccc ggagatcgcc caggagacgt gggatgagat cggcaaaaac ggccctgtct
    27841 ttttcggctg ggccggcggc agggagcgcg gggagccgca ctactaccgc gtgcagggcg
    27901 cgaccttcct cattgagtac gacaatgtgc agggcgaggc caaccacccg cacagcgtct
    27961 tccgcagctt cgacggtgac ttcggccgcg acctgctggg cgagcaccac cgcgagcagc
    28021 acggcaagaa gtgaagcggc aggaactgat gcccgcccat gctccggcac gccctttttg
    28081 cagcgctcct gccgctggcc gccgctgccg cgccggagat cccgctggcg cacggcccgc
    28141 accgggacct ggcgctccag gcgctgcctg actcgaccct ggagatcagg ctcggcggcg
    28201 gccacccgca cttctggaca gccgtcgtac ccgccggcta tgaccccgcc cggcaaagca
    28261 tcctggcgct ggactacttc gccccctccg gactcgaatc cgtggtgctg cgctaccgcg
    28321 cccagggggg tgacatggtc gtggccgagg cgcgccagac cggcatcgcc gaggcgtggc
    28381 agccgctcgt ttttgacctc tcccggctcg acccaccacc cgccgccggc cacccggaga
    28441 tgcgtttcca tttcgccctc aatggcgcgg cggagagcct cctgcgcttg cgccatctgc
    28501 gcttgcgcgc ccccaccgcc gcagaggcgc gcctggctgc ggagcgggat caaacgctgg
    28561 ccgcgcgcga ggctgacgcc gccgccatc
//
LOCUS       DAOMYJ010000041        28533 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Prosthecobacter sp. isolate AMR_MDS_5629
            NODE_586_length_28533_cov_21.971381, whole genome shotgun sequence.
ACCESSION   DAOMYJ010000041 DAOMYJ010000000
VERSION     DAOMYJ010000041.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867850
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prosthecobacter sp. (wastewater metagenome)
  ORGANISM  Prosthecobacter sp.
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Verrucomicrobiaceae; Prosthecobacter.
REFERENCE   1  (bases 1 to 28533)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 28533)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.30
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:34:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,742
            CDSs (total)                      :: 4,684
            Genes (coding)                    :: 4,676
            CDSs (with protein)               :: 4,676
            Genes (RNA)                       :: 58
            tRNAs                             :: 55
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28533
                     /organism="Prosthecobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_586_length_28533_cov_21.971381"
                     /isolate="AMR_MDS_5629"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1965333"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..440
                     /locus_tag="PLP58_06480"
     CDS             <1..440
                     /locus_tag="PLP58_06480"
                     /inference="COORDINATES: protein motif:HMM:NF014628.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="LUD domain-containing protein"
                     /protein_id="HRJ08196.1"
                     /translation="RALLAQVYPGREIAAALPAGQVIASTVPGLPGTLDPFAVSDPHD
                     LRGVDTAVVPGRFGVCENGAVWLDEGALGPHRVLPFIAQHLVLVLPARELVASMHDAY
                     ARLGAPDTGFGVFIAGPSKTADIEQCLVIGAHGARSCTVWLLD"
     gene            complement(447..1340)
                     /locus_tag="PLP58_06485"
     CDS             complement(447..1340)
                     /locus_tag="PLP58_06485"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF024545.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HRJ08197.1"
                     /translation="MALIINGERVDDEIIESEFNQVKGHYERMLQVSCCERDAEFRAT
                     AKDNVVSRALLNQEAAKRFPEVSEEDVSSRLEKLIEQAGGETRFYMSIGMHVKNEALV
                     RQNVAGGVRLDKMMAEIYGPDPDIGEDELRAHYEQHTGQFMSEELIHAAHITKSMQGA
                     QSRAEVYETLRGLRRELQAGADFMELAEKHRAGDQQQIDLGWFKRGEFMEEFEAIAFS
                     MNPGEISPVFVTQLGFHICTVLERKAAEPLPFPAVKDAVRERVLETRKDLLFHEFIEG
                     LKAAATVEDTDPDEPDCGCAH"
     gene            complement(1437..1931)
                     /locus_tag="PLP58_06490"
     CDS             complement(1437..1931)
                     /locus_tag="PLP58_06490"
                     /inference="COORDINATES: protein motif:HMM:NF012621.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HRJ08198.1"
                     /translation="MKKLALLLSLSLSTLAPAATTVTLEGVHNCCRSCANGIVKAAAG
                     IKDVEVTAEGSTITITAKTKTSAKKAVEAIMEAGYFGKSSEESFTSAPKADKTLKEAT
                     VTGVHLCCQKCVDAMSKAVKAVSGVQEAEIANKATSFTVKGEFSSQALLAAMNQAGFH
                     GTIK"
     gene            complement(1995..2489)
                     /locus_tag="PLP58_06495"
     CDS             complement(1995..2489)
                     /locus_tag="PLP58_06495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ08199.1"
                     /translation="MPIQPSNPRVLMQQLDSGRISHEEFRQAMSEHAREIIVEMEEDR
                     ANPAVAFLEQMLSRRAASRLLKKNEESVIREVLAALAEVEDFPPGRWLWNAAHPHIPL
                     HAFFRTRREPVFRITQMEAMPQVVSVTLEHGPADKSLVAREEFRMRRDRRGRLGVEHR
                     RRLA"
     gene            complement(2543..2854)
                     /locus_tag="PLP58_06500"
     CDS             complement(2543..2854)
                     /locus_tag="PLP58_06500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ08200.1"
                     /translation="MPIYVLRDGEAPKPAGWAAYLRAAAMILLIVALAAVVIFVGLAV
                     ALIAVAVSAVAALVYAVRRRLAGGSKPRSAPVEPQTAASTQPAGGEARVIEVDEIIVR
                     R"
     gene            2952..3140
                     /locus_tag="PLP58_06505"
     CDS             2952..3140
                     /locus_tag="PLP58_06505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ08201.1"
                     /translation="MNTRKGPKIPQMLIQLRQLIQADSGPALVRRRDLRDVAQGEIQL
                     ELDLSPHGRVQSGILTRQ"
     gene            3237..3887
                     /locus_tag="PLP58_06510"
     CDS             3237..3887
                     /locus_tag="PLP58_06510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ08202.1"
                     /translation="MNPQTDALEHLRVIRSLMEKAHIYRAISAPAALAGGLLALALSG
                     WQAARAFRDLAPADPCSFAAQWLAVLMVTSVLNFALLSREARRRGQPFVSDGMRAALR
                     ALCPPLLAGGVLGLGLMLRLHNLTLGALVWVVCHGLALLATAGFSPRSLIRLGWAFLT
                     AGLALFLIWAVRDDIRLLPSDEGPAAVIMGLTFGLLHLIYAAAVFARRGSEGRTGA"
     gene            3884..4186
                     /locus_tag="PLP58_06515"
     CDS             3884..4186
                     /locus_tag="PLP58_06515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009964023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HRJ08203.1"
                     /translation="MIDFDQLDKTIHEKGRLSIMTLLAGRAGWSFQDLKQELGMSDGN
                     LITHLRTLAAAGYTEEKKDGTGSRPRTTYHLTAAGKLAFEEYLDVLESIVKTLRPD"
     gene            4240..5079
                     /gene="ispH"
                     /locus_tag="PLP58_06520"
     CDS             4240..5079
                     /gene="ispH"
                     /locus_tag="PLP58_06520"
                     /EC_number="1.17.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009964024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051745 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate reductase activity [Evidence IEA];
                     GO_process: GO:0009240 - isopentenyl diphosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0015968 - stringent response [Evidence
                     IEA];
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-enyl diphosphate
                     reductase"
                     /protein_id="HRJ08204.1"
                     /translation="MKIHLATHHGMCFGVRDALRAAHDAAMREPVTLLGQLVHNPVVD
                     RHMRSLGARPGALDGGRADTRRVVITAHGASEKDRLHWREAGHLITDTTCPLVRKAHE
                     ALAMLVREGCHPVVIGQANHVEVRGLTGDFPGAFVIETGADAAALPFRERYGIVSQTT
                     QPLERVLALVEVVKRRHPGAQARFMDTVCRPTKLRQEALEDLCVRCEVVIVIGGRASN
                     NTRQLLEKALRLGVRAHLVETAADLLPEWLRGAENIGVTAGTSTLDETVRAVMDRLQT
                     LAC"
     gene            5118..5888
                     /locus_tag="PLP58_06525"
     CDS             5118..5888
                     /locus_tag="PLP58_06525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HRJ08205.1"
                     /translation="MNTGHWIRHFTRNTPLNDSLRLPPAPVTLPDEIRLPLAASLAVF
                     QLGESGGGTRLRRYAREVAPLVNFRGYQRAVDLFIAEEQSHSRLLERVVLHLGGSLIE
                     KQWTNSVFRRLRGLVNLEFAIQVLLTAELVAEIYYGALYLRVSDPVVRVMARKILQDE
                     TRHLEFQREFLSERLATFSPAGRWLWRLQFEAVHAVTAAVVAWDHRHCLKALGVGLPE
                     FFERTRKAWAVFQRRLDSRIRVLRECGGVAQVLSPTNP"
     gene            complement(5918..6607)
                     /locus_tag="PLP58_06530"
     CDS             complement(5918..6607)
                     /locus_tag="PLP58_06530"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009958989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HRJ08206.1"
                     /translation="MRRLDQLLSSLGYCSRREAAALVKAKRVKQNDIVLTKADQKVDP
                     HAITLDGEPLEAPDGLLAMLHKPVGYVCTHNDGEGPTIYELLPDQWLRRNPAVTSVGR
                     LDKDTSGLLLVTDIGSLVHRFTSPKADVEKVYLAELDRELDPQLVEIFARGDLMLRGE
                     DKPCLPARLEIVSSQTARLTITEGRYHQVRRMFASQGWHVEKLHRERFGPHDLGDLAE
                     GEWRLIPVDTL"
     gene            6651..7382
                     /locus_tag="PLP58_06535"
     CDS             6651..7382
                     /locus_tag="PLP58_06535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zeta toxin family protein"
                     /protein_id="HRJ08207.1"
                     /translation="MSSTICIIGGCNGAGKSTLARELLPRLGIGRFLNADLIAKGLSP
                     VNPELAAFSAGRRLLEEAQSLIEAGSSFALESTLSGKTYVKLLREAKERGYRLVLHYI
                     VIGSATQAVERVRLRVLTGGHHVPEDDIRRRFERSVRHILEDYLPLADEWGLWDNAYV
                     PARQIADNSSHTLQQVRDMITSTNLQETPPMPSTEMSQIVLEASRVATAKMLDLYERM
                     GIKVTPEMTLAPDSPPPAFKPFEFW"
     gene            7473..8945
                     /locus_tag="PLP58_06540"
     CDS             7473..8945
                     /locus_tag="PLP58_06540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ08208.1"
                     /translation="MKTTPFVLCLALAAMLRAAPPPDEPVEIGTTPQFFVDDHLVDNR
                     WALKQKREMVLRVFHAPVKHAGNPLLSGETGFCSVVKDEQSGMFRMWYQTHAWTGKGP
                     EDESKTQYGIAYAESKDGVKWEKPKLGLHEWLGTKENNIVYKGFMNSRASGPQILRLP
                     EADRRGFKYVLTYRTSGAKRGNNGVRVVGSQDGIHWDEKSDTLICQFPSDTLNSIVYD
                     TTRKEYVMFCRAKNRYRIFEGDILDTGESRRIARVSHPGLWEEWKPVPQAILTPDELD
                     ARENFNAFYGMPVRYHAGIYWGALWCFRFNDNIFTEPVFSRDGFDFDRLPDRPKILPL
                     GEEGSWDDGMTFGSMDWVEMGDEWWYYYSGWDGDHGGKERNAAIGLARGRKEGLVSLR
                     GPRGGGVVVTRLLKWPGGKLMLNADAAKGELKVRVSDATRKVIPGFDYTDCEPFTGDS
                     TALEMKWRGQSLDALAGKELRFEFFLREADLFTFRADTSK"
     gene            8982..9920
                     /locus_tag="PLP58_06545"
     CDS             8982..9920
                     /locus_tag="PLP58_06545"
                     /inference="COORDINATES: protein motif:HMM:NF024640.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terpene cyclase/mutase family protein"
                     /protein_id="HRJ08209.1"
                     /translation="MKTRLILCLLAAATAASAEEALDIAKRSLPFIKEKGLAWIEDRQ
                     CASCHQIPSMLWSLNSALKAGLITESPEVVKWTPWAADWRHWNQTKDKDGVDKVSAGN
                     IDTMVFLLLGSSDPAPAWAADFRAQLLKNQQPDGSWKAGGQLPLGKRPPREIHEVTTM
                     WTLLALKPTADDPVRKRAEDYLAKAQPGQSTEWHAVRLLLKPDDAALREALLAFQHED
                     GGWGWLTNEPSDAFGTGLALYVLAKSGLPADHDSRKKAVAFLKSTQRPDGSWPVPSTR
                     ARDKNKINKTATYWGTAWAVIGLLEDPAVTGVSAAR"
     gene            9929..10885
                     /locus_tag="PLP58_06550"
     CDS             9929..10885
                     /locus_tag="PLP58_06550"
                     /inference="COORDINATES: protein motif:HMM:NF024627.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HRJ08210.1"
                     /translation="MKQALFFLLTLALHSQAQIGVQGTVGAPAGAKVVNRLEITKPGV
                     YENLIIDGNFARGNLVKITADNVTVRNCEIRHSAGNGIGIFGNKVVIENCRIHHLLNG
                     TFEDQQDAHGISGRWGDTIIRNCDISFPSGDCIQFDPDRKSTGKVVIEQCTLWTAPLD
                     KDMAGFKAGQRPGENAMDTKTMPDGPRCQLLIRNCHLHGWNQPAQIDNVAALNLKENV
                     DAEVSGCVFQNNEIALRVRGPGKRGGAHVIATDCAIYDTQTGIRAEDMIEVLKLTKIG
                     FGSDIGKRMQFVGGKSDSGIQITGEQDAPAVDGLLKKGFPER"
     gene            10893..11465
                     /locus_tag="PLP58_06555"
     CDS             10893..11465
                     /locus_tag="PLP58_06555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ08211.1"
                     /translation="MAWRFDKCVINGEIDNTVRGRVTGKLWLLGREEPMILDLVGDAW
                     PDLAGCRLTFVNTNPVPQPQYDEGLSMRQTGSVGDITASMKLKKLLVPEEEWMKAIEE
                     KRFHEVPWVLSSSLYLEWFSDRNGRVVIQTTDYKLEVSERQWEADADDQAAQQAINDE
                     NMRAYMEDLGRAFGGQEDGQEERKEGEDRD"
     gene            complement(11527..12039)
                     /locus_tag="PLP58_06560"
     CDS             complement(11527..12039)
                     /locus_tag="PLP58_06560"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015679.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_function: GO:0071972 - peptidoglycan L,D-transpeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HRJ08212.1"
                     /translation="MASIACLRLDRANHAGMSAASPRIEVSIGSQTLALHEGARLLKQ
                     WPCSTSKFGIGFAEGSNRTPLGRFVIREKHGGGAPSGTIFKARKPVGQWQPGLDTRED
                     LVLTRILRLHGLEKRNANTFQRYIYIHGTNDERSIGRPASHGCVRLRNADVVELFDLV
                     TEGAEVWISP"
     gene            12133..15510
                     /locus_tag="PLP58_06565"
     CDS             12133..15510
                     /locus_tag="PLP58_06565"
                     /inference="COORDINATES: protein motif:HMM:NF015531.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HRJ08213.1"
                     /translation="MIHHPLFPLSLLACMALSAAAEPVRLAQTPVVSPDGSLVAFSWR
                     GDIWTVPAKGGSARRLTTHPALDTTPVFSPDGGQIAFLSEREGGRQVHVMPARGGQPR
                     QLTFHTEGYDLAAWMPDGRELLVTLVRDHSWMRGSRSARMALLDVSERKAERVLLDDA
                     AAECSVSPDGKKILFVREGEMWWRQGYRGARAGQIWLLDRGSGECRQVKAEATECRFP
                     LWKPDGSGFYFTSNRDGAFNLCEHDLASGRTLQITRFKTDSVVFPALSKDGGTLVFRH
                     LADLYRWHPGQDKAPEKIRVNHTGDAPDAPVQRRVLERATGIAHTANGLQMAFIADGD
                     VWVMDTELREPRQVTRTPEEERDVVFAPDGKALWFVSDSGGQADIWKALPAAPAKAWW
                     ENTEFKLERVTSDPAVESAPRFSPDGKRLAWLKERGDFWLADADGKNARRVFASWNKP
                     SFDFSPDGKWIVYSQSDEWFNSDIWLLPLDGSKPPFNLSRHPNNDYSPVWSPDGKMIA
                     WTGAREIDEVDIFYVWLRAQDDEQSKRERTAIKAREKLAKASAAPAAKSPATTPPAKP
                     AEPPAPAPAKPGQADPKAAAKAEPQAESKAAAKSKTAETPKPKAPTAPATLQMDLEDI
                     HERIRRISIPNTSESGLTWSHDSKKLAFTATVEGKRGTYTVEFPDDLKPKMLSSTTLT
                     DARWIKEGNQILGLNETNTPSAISSTGSLTTWRFRALQSTDTAGRQRAVFDQCWRIMR
                     DHYYDEKLGNRDWDAVRAKYSGLAAEAPDMRSVQEIVHLMLGELNGSHLGFSLNTAAA
                     SSGTWREETAHLGLRFDDKHPGPGWKVRDVLPNGPASHRLRRILPGEIILRIDGRETR
                     PGQDSSELLNGPLERDITLRARAADGKERDVTLRPISFSTARGLLYEHWIKDNRRRVE
                     QLSGGKLGYLHISAMDSASFHKFQAELYAAGAGRDGLIIDVRENGGGSTTDHLLTALT
                     QPRHALTIPRGGSAPGYPQDRTIYATWDKPVAVLCNQNSYSNAEIFSHAIRVLGRGQV
                     VGVPTAGGVISTGSARVMEAGTLRLPFRGWYGIESGLDMELNGAEPHHILWPLPGDAA
                     RGVDRQLEKAVEVLKSDVEAWATRPRPKLLKASERKF"
     gene            15601..16590
                     /locus_tag="PLP58_06570"
     CDS             15601..16590
                     /locus_tag="PLP58_06570"
                     /EC_number="1.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009960867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016615 - malate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006108 - malate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HRJ08214.1"
                     /translation="MSKAPITVAVTGAAGQIGYSLLFRIASGALFGPDQPVNLRLIEI
                     EPAMGALGGVCMELDDCAFPLLNSITPTCDLDEGFKNTNWAILVGSVPRKAGMERKDL
                     LNINGKIFTGQGKAIEKNSASDVRVLVVGNPCNTNCLIAMNNAKGVPAERWFAMTRLD
                     ENRAKSQLAAKSGAHHSSITNMTIWGNHSATQYPDFYNAKIGGKPATEVISDHEWLQG
                     EFIATVQQRGAAIIKARGLSSAASAANAACDTVHSLTRPAAAGDWTSVAVCSDGSYGI
                     EKGLMFSFPIRTDGARWEVVQGVPVNEFSQAKIKATEDELKEERDAVRELGLI"
     gene            16723..18180
                     /locus_tag="PLP58_06575"
     CDS             16723..18180
                     /locus_tag="PLP58_06575"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase"
                     /protein_id="HRJ08215.1"
                     /translation="MKSAPRLAAAILGAACLSGLQAAPNILFVVADDLNCAIGPYGDK
                     SAVTPNLDRLAARGLVFERAYCQQAVCNPSRASFLTGLRPATTGVDDLRKHFRDTAPG
                     GKKLVTLPQHFKNHGWFCQNIGKMFHNMGDTQDRQSWSMDETMHTGTHAADTVFANTP
                     LKPGDPAPEYKAPVTEAPDAPDTAYRDGQIARLAAGVIRDRAGSAQPFFLAVGFWRPH
                     LPFVAPKKYWDLHDPAALPSAEPPAPPRDAPAIALHESREIRGYGGVPKDRPFTETEA
                     RHYRHGYHASISFLDAQLGLILDALEQSGQADKTIVVFTSDHGFHTGEHALWGKTSNF
                     ELDARVPLIIADPRQPAGHGKKTRALAELVDLYPTLAGLAGVAQGLPGNLEGASLAPV
                     LADPAASVKDAAHTWHQHPFYGPASAWQAWGCSMRTQRWRYTEWRSIQSREVIARELY
                     DHDSDPLETVNLASQPAHAARVSELAGKLAIQFPR"
     gene            complement(18190..19242)
                     /locus_tag="PLP58_06580"
     CDS             complement(18190..19242)
                     /locus_tag="PLP58_06580"
                     /inference="COORDINATES: protein motif:HMM:NF024627.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HRJ08216.1"
                     /translation="MTRFLSLVLLGSTLSAAGHVARDERELRAVLAGLKSGDVVKIAP
                     GIYPGGHHVSGVENLTVEALDKERPPVFEGGRGGWHFSACPGLVLRLIHVRGQSVNGI
                     NLDEGGAGRPLVKGIRIEGVQVSDIGPSGNHDGIKASGLEGAVIQDCVITGWGGQGVD
                     LVGCHAVVISGCRFTGKEGFTATAGVQIKGGSSGVVVEKCRFENAGERPVNIGGSTGM
                     PFFRPPGAKHEAKDITVRGNHIEGSLCAAAFVGVDGALFEDNTVLYPARWIFRILQET
                     RAEGFTPCRGGIVRGNRVVFRRADIREDVNMSPGVAAETFRFENNRWFAEDRPLASKP
                     RLPVEETGGEHGTDPR"
     gene            complement(19297..21546)
                     /locus_tag="PLP58_06585"
     CDS             complement(19297..21546)
                     /locus_tag="PLP58_06585"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HRJ08217.1"
                     /translation="MSSTKARPSPLTFLRLIAALLVAASAHGQEPAPLTTVAQVLDGG
                     VSQTGQTARAVRVKGVVVSVSGMFNFFYLHEGGRCVAVRYPRRLAPPALGDRVEVEGS
                     TMVNMLGGLRQMRVSADEFIITGSGDLPVPEPATLEKLLSDSAMDQWLSCQARVAGWR
                     LDGTDLRLRLVLDGGVFDGFVSLPGAGAPPDKLHGARLRLTGVVTFAPVLGRLIFVPG
                     ISQFEVLETGVNDPFEAETIPASQVSSEANAGCRPARVRGIVLGRTSDGAIHIRDDGT
                     ALRAALRQPWSGEERTGARARETAPAAGDEVEITGFSHDARFGETTPPGCDLLECVLR
                     LTGRKEPPQPVTAALAEIAAGERTSDWVETRGRLVTLHQVPLENGEWRSTLMIEADGA
                     RMPVAFRAGGRPAFDTIKPDDDVLVRGLVERATALSPRQMRLLDDADVRSLGVSAVVR
                     ARQLWLWGGGTLAVIALFSGWIAALRKSNRVKTRVAALLEKRVAERTAELEKAQAEIT
                     RALAQERELGDLKSRFVTMVSHEFRTPLGIIMSAIELMRHYNDRLPPDQRLELQNDIF
                     TSTRHMAGLMEQVLVLGRVEAGKLGCKTAPCDLDILAGKIVDENLSATNRKCPVIWRS
                     EGGLDGARADESLLRHIFSNLITNAVKYSPEGGEVLLTARREGGDAVFQVVDHGIGIP
                     ENERGRLFEAFHRCSNVGETPGTGLGLVIVKRCVELHGGSVHVESATGKGTAFTVRLP
                     LFAGQDDGK"
     gene            complement(21554..21922)
                     /locus_tag="PLP58_06590"
     CDS             complement(21554..21922)
                     /locus_tag="PLP58_06590"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HRJ08218.1"
                     /translation="MPRKILIIEDQATMRRNVALMLQLEGHTALTAANGIEGIETARR
                     ERPDLILCDVMMPEMDGHAVLRALHEDETLRGTPFVFLTAKGDKSDVSEGLGLGADSY
                     LTKPVSREDLLSTVNEHLKT"
     gene            complement(21930..24632)
                     /locus_tag="PLP58_06595"
     CDS             complement(21930..24632)
                     /locus_tag="PLP58_06595"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing sensor histidine kinase"
                     /protein_id="HRJ08219.1"
                     /translation="MKIIRQLIWGQQCLRALLLIVTASAASAQTMPDDIFAAPLVTIK
                     DLNARKTGLDRRVRVRGALITIMESRTLILHDGSGAAAVLAGDPASASPEVAGRGVPL
                     PPMQPGDVIEAAGEVVDQTDYNAKFLGMVRCHARVVGRGAVPEPEEVSLETFLAYRDE
                     ARWVTFEAVVHAWMLQASAMTYAAGDDRTWTTVSVRSPDLAAFPKNLFGARLRFTGLA
                     TGLAINFRGADMIVPGPAFVELVKPGRDSPFGAPFHPASEIAAGRAPKGEPVSTKGVL
                     VGQERTVLYIRGEDASLCVSLQQPWARPGNPPGIAYADCGPLPALKIGDELEVSGHQF
                     TTERYEPFDLASASVRVTGHRENIEPVETTLARIAAGGHTSDLVKVRARLLSLQAARE
                     KEGKWRATLLLASEGRRMTAVHQSAAPHSFETLNEGDDVIIQAVVDRATSTSPRLLWI
                     LKPSDVESLGVSFEVVTRRLRLWGGIALAVFGILGGWVLLLRRTQLRQARMTAELKTA
                     KDAAQASERRWKLLFEQSPLSVQIFAPDGRTKLFNDAWRRLFRLSDEEGLAFNVLQAP
                     DLIESGAVNHIRRAFEGEVVQVPPVPFPVPGDPPETRWIGGLLYPLKNEAGEISEVVV
                     IHHDITEMKRAEQAMLEINQTLEKRVNERTGELQRAQSELRRALEQERELGELKSRFV
                     TMVSHEFRTPLGIIMSAVELLQHYSDRLPEDEKKRQLQEIQSSTKHMGGLMEQVLLLG
                     RAEAGKLTCKPAPLELAAFAERVIDETQSITSGKCRVTLDTEAVPASSNVDESLLRHI
                     LSNLVANAVKYSPEGSEVTLRVRCRGPQAVFEVSDRGIGIPEKDRARLFEAFHRCSNV
                     GDTPGTGLGLVIVKRCVELHGGSIEVASEPGLGSTFTVRIPILN"
     gene            24691..25329
                     /locus_tag="PLP58_06600"
     CDS             24691..25329
                     /locus_tag="PLP58_06600"
                     /inference="COORDINATES: protein motif:HMM:NF013250.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HRJ08220.1"
                     /translation="MPPPLHSSRGGLSIRSRTPDQRGRLRVTFATQITIFRILLIPVF
                     VGTAVYYGQSVAAGAPNESLRAWTIAVFALAAVSDAVDGYVARHYNQRSRLGAILDPL
                     ADKLLLLSAIVTLSFTGWRQQFPLWFPTLVIFRDLASIGGAFLIQHLAGSCHIQPHWT
                     GKVATFAQIAAVLWLMLEFPFLLWPTVVAGVFTALSGFINLASGIRQVRHVG"
     gene            25424..26803
                     /gene="der"
                     /locus_tag="PLP58_06605"
     CDS             25424..26803
                     /gene="der"
                     /locus_tag="PLP58_06605"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012419376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HRJ08221.1"
                     /translation="MKTVAIVGRPNVGKSALFNRLAGMNISIVHDMAGVTRDRITAEC
                     RRGREVFEIMDTGGIGANTSDILTEQVQTEASIALEVADLLLFVVDVHDGVTPIDQAL
                     AQVLRRVNKPVVLVINKADSDKRRLGSGEFSRLGFPDSAEVSAVHGLGIKELVELVSE
                     KLGLEDEETEEVVEQKRAGRPLKLAIVGRPNAGKSSLVNAILGQERAIVSEVPGTTRD
                     SLDIHCEFNGRRYQLIDTAGIRRQARLEEAVEIFSVQRSYQAIKRADICLLVIDCAGG
                     AKMQDRKVAQLILEERKPCILVMNKYDLYHPSAKQKDRIEELEETMRREFFFLNYAPL
                     VAISAKKREHIGKLFVQIEAVRRGAQTRIGTGALNRLLHEAIANTPGPLGRSSHAFKL
                     LYATQVNSPEEEQVPVPHFVLFANRAAKLTDSYLRFLESVIRKEWPAPGVPFRMSVRG
                     KEPKEKGRR"
     gene            complement(26816..28519)
                     /locus_tag="PLP58_06610"
     CDS             complement(26816..28519)
                     /locus_tag="PLP58_06610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009961275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GspE/PulE family protein"
                     /protein_id="HRJ08222.1"
                     /translation="MRQALVQCLEASGGVGIALPTATPSASVVNQLLDTGKIDEEAFL
                     SQLAARIGLGFVPNPLPDSADAPEMKRLLPPRVALKHRLLPLKIQEPAAEGCDRKLVI
                     AAYDPFDLIARQAVAREVDAPVRWEIAPRKRLLNAMRDFYGVGADTFEEILKGRSVDD
                     ADLEQRDEANVIDDDDEEASVVKFVNTVIREALEQHATDIHVEPMEDKLRMRYRIDGR
                     LREVPVPENIKALQQSVIARLKVMAKLDIAERRLPQDGRINLQVGGQFIDVRVATIPS
                     VEGESVSLRLLGQEKFNLDRLRIESDLRAEVETLLKKPNGIILVTGPTGSGKSTTLYT
                     FLSQLNTEHRRIVTIEDPVENKLPGVVQIAVRPEINLSFATGLRSILRGDPNVVMVGE
                     MRDLETAEIAIRAALTGHLVFSTLHTNDAIGGITRLVDMGVEPFLVASSVRAFIAQRL
                     VRVLCSDCKEIEPDAQAKLHELKYHGPVGAPVYRAREGGCEACRATGYKGRMSIYELV
                     RLTPAMGELITHKASGQQLLQQALKDGYRPMREYGVRKILAGLTTIEEVISVTVAESE
                     Q"
BASE COUNT         5013 a         9441 c         9110 g         4969 t
ORIGIN      
        1 atcgcgcgct cctcgcccag gtctaccccg gccgggagat cgccgccgct ttgcccgccg
       61 ggcaggtcat tgcctccacg gtgccggggc tgcccggcac gctcgatccg ttcgcggtca
      121 gcgatccgca tgatttgcgc ggggtggaca ccgccgtggt gccgggccgc ttcggcgtgt
      181 gcgagaacgg cgcggtgtgg ctggatgagg gcgcgctggg accgcaccgc gtgctgccat
      241 tcatcgcgca gcatctcgtg ctggtgctgc ccgcgcgcga gctggtggcc tcgatgcatg
      301 atgcgtatgc ccggctcggc gcgccggaca cgggcttcgg cgtcttcatc gccggaccgt
      361 cgaagaccgc ggacatcgag cagtgcctcg tcatcggcgc gcacggggcg cgcagttgca
      421 cggtgtggct gctggattga gcggcgtcaa tgcgcgcagc cgcagtccgg ctcgtccggg
      481 tcggtgtcct cgacggtggc ggcggctttg aggccctcga tgaactcgtg gaacagcagg
      541 tccttgcgcg tctccagcac gcgctcgcgg acggcgtctt tgacggcggg gaagggcagg
      601 ggctcggcgg ccttgcgctc cagcacggtg cagatgtgga agccgagctg ggtgacaaag
      661 acggggctga tctcgccggg gttcatggag aaggcgatgg cctcgaactc ctccatgaac
      721 tcgccgcgct tgaaccagcc gaggtcaatc tgctgctggt cgccggcgcg gtgcttctcg
      781 gcgagttcca tgaagtccgc ccccgcctgc agctccctgc gcaggccgcg cagggtctcg
      841 taaacctcgg cccggctttg cgcgccctgc atgcttttgg tgatgtgcgc ggcgtggatc
      901 agctcctcgc tcatgaactg gccggtgtgc tgttcataat gggcgcgcag ctcgtcctcg
      961 ccgatgtccg ggtccgggcc gtaaatttcg gccatcattt tgtccaggcg cacgccgcct
     1021 gccacgttct gccgcaccag cgcctcgttt ttgacgtgca tgccgatgct catgtaaaag
     1081 cgcgtctcgc ctcccgcctg ctcgatgagt ttttcaaggc gcgaggacac gtcctcctcg
     1141 gagacctcag ggaagcgctt tgccgcctcc tggttgagca gcgcgcggga gacgacgttg
     1201 tccttggcgg tggcgcggaa ctcggcgtcg cgctcgcagc aggagacctg gagcatgcgc
     1261 tcgtagtgcc ccttcacctg gttgaactcg gactcgatga tttcatcgtc cactctttcg
     1321 ccgttaatga tcaatgccat ggggtgcgcg cgcgggatgg agttggggga aaaataaaga
     1381 ggcaggcccg gctgccggcg cccgcctcgt ggggatttgg ccgcaggccg gcggggttat
     1441 ttgatcgtgc cgtggaaacc tgcctggttc atggcggcga ggagcgcctg cgaggagaac
     1501 tcgcccttga cggtgaaaga ggtggccttg ttggcgatct cagcctcctg cacgccggac
     1561 acggctttca cagccttgct catggcgtcc acgcatttct ggcagcaaag atgcacgccg
     1621 gtgacggtgg cctctttgag ggtcttgtcg gccttgggcg cggaagtgaa ggactcctcg
     1681 ctgctcttgc cgaagtaacc ggcctccatg atggcctcga ccgctttctt cgcgctcgtc
     1741 ttggtcttgg cggtgatggt gatggtggag ccttcggcgg tgacttccac gtccttgatg
     1801 ccggcggcgg ctttgacaat gccgttggcg cagctccggc agcagttatg cacgccttcg
     1861 agcgtcacgg tcgtggcggc gggggcgagg gtggagagcg acagggagag gaggagggcc
     1921 agttttttca tgccctgaca agacgcgcgc ccgcctggct tcttagcagg cttttttcac
     1981 agcatccgct gccctcacgc cagccgccgc ctgtgctcca cgcccagcct tccgcgccgg
     2041 tcgcgccgca tgcggaactc ctcccgcgcc acgagactct tgtccgccgg gccgtgctcc
     2101 agcgtcacgc tcaccacctg cggcatcgcc tccatctgag tgatgcggaa gaccggctcc
     2161 cgccgtgtgc ggaagaaggc gtgcagcggg atgtgcggat gcgccgcgtt ccacagccac
     2221 cggccgggcg ggaaatcctc cacctccgcc agagccgcga gcacctcgcg aatcacggac
     2281 tcctcattct ttttcaaaag cctggaggcc gcgcgccggc tgagcatctg ctccagaaag
     2341 gccaccgcag ggttggcgcg gtcctcctcc atctcgacga tgatttcccg cgcgtgctcg
     2401 ctcatggcct ggcggaactc ctcatggctg atgcggccgg agtcgagctg ctgcatgagc
     2461 acgcggggat tggaaggctg gatgggcacg gcaatgattc aagggaagcc ggcggggatg
     2521 gcaagatgga aatttccgct catcaacggc ggacgatgat ctcgtccacc tcgatcaccc
     2581 tcgcctcccc cccggcaggc tgggtgcttg cggcagtctg aggctccacc ggcgctgagc
     2641 gcggctttga tccgcccgca aggcggcggc gcaccgcgta aaccagcgcc gccacagccg
     2701 agacagcgac cgcaatcagc gcgacggcaa gtccgacaaa aatgacaact gccgccagcg
     2761 cgacgatgag caggatcatg gcggcagcgc gcaaataagc cgcccaaccc gcaggcttcg
     2821 gcgcttctcc atcgcgaaga acataaatcg gcatgccatc atcatgagcc ggctttgaga
     2881 aagcacaaca gcggaggaaa atgaaactgt tcgattgatt ataattcgag cggccttatt
     2941 aaagggttct catgaacact cgaaaaggcc cgaagattcc gcagatgttg atccaactac
     3001 gtcaactgat ccaggccgat tccggtccgg cgctcgtcag acgccgcgac ctgcgcgacg
     3061 tggcgcaggg tgagattcag ttggagctgg atctctcgcc gcacggcaga gtccaatcag
     3121 gaatattgac caggcaatga caggctggct gaattccgga ccgggaagtg aaaaaatcac
     3181 tttgcttcac aaagttattt gttgcgcgtt tcactttttg tcacaaagta aaacccatga
     3241 acccgcagac cgacgccctg gagcaccttc gtgtgattcg ctcgctgatg gaaaaggcgc
     3301 acatctaccg cgccatttca gctccagccg cgcttgctgg cggcttgctc gcattggcac
     3361 tttccggctg gcaggcggcg cgcgcgtttc gcgatctggc tccggccgac ccatgctcct
     3421 tcgccgcgca atggctggcc gtgctgatgg tcacctcggt gctgaacttc gctcttctca
     3481 gccgcgaggc gcggcggcgc gggcagcctt ttgtttcgga tggcatgcgc gccgccttgc
     3541 gcgccttgtg cccgcccttg ctcgcgggtg gtgtgctggg cctggggctt atgctgcggc
     3601 tgcacaatct caccctgggc gcgctcgtct gggtcgtctg ccacggtctg gctctcctgg
     3661 caacggcggg cttttctccc cgttcgctga tccgcctggg ttgggcgttt ctaaccgccg
     3721 ggctggcgct ttttctcatc tgggcggtca gggatgacat ccgcctgctg ccctcggacg
     3781 aaggcccggc cgccgtcatc atgggtctga ccttcggcct gttgcatttg atttacgcgg
     3841 cggctgtttt tgcgcggcgc ggcagcgagg ggcgaacagg cgcatgattg acttcgacca
     3901 gctcgacaag acgatccacg aaaaggggcg tctttccatc atgaccctgc tggcgggcag
     3961 ggcggggtgg tcgtttcagg atttgaagca agagctgggc atgagtgacg gcaatctcat
     4021 cacccacctg cgcaccctgg ccgccgccgg atacacggag gagaaaaagg acggcacggg
     4081 atcgcgcccg cgcacgacct atcatctcac cgccgcggga aagctcgctt ttgaggagta
     4141 tctcgacgtg ctggagagca tcgtgaaaac cctgcggccg gattgatctc atttttgcgc
     4201 ctatttactt tgtatcacaa agcacaaacc cacacactca tgaaaatcca cctcgccacc
     4261 catcacggca tgtgtttcgg agtccgcgac gcactccgtg ccgcgcatga cgccgccatg
     4321 cgcgagccgg tcaccctgct cggacagctc gttcacaacc cggtcgtgga ccgtcatatg
     4381 cgctcccttg gcgcgcgtcc gggtgctctg gacggaggac gcgcggacac acggcgcgtg
     4441 gtcattaccg cgcatggcgc gtcggagaaa gaccggctgc attggcggga agccggacac
     4501 ctgatcacag acaccacctg cccgctggtg cgcaaggccc acgaggccct ggccatgctc
     4561 gtgcgcgagg gctgccaccc tgttgtcatc ggccaggcaa atcatgtcga agtgcgcggg
     4621 ctgacgggcg acttccccgg cgcgtttgtt atcgagaccg gggcggatgc agccgcgctg
     4681 ccgtttcgcg agcgctatgg catcgtctcc caaaccacgc agccgcttga gcgcgtgctg
     4741 gcgctggtgg aggtggtgaa gcggcgtcat cccggcgcgc aggcgcgctt catggacacc
     4801 gtctgccgcc ccacgaaact gcgccaggag gcattggagg acctgtgcgt gcgctgcgag
     4861 gtggtcatcg tcatcggcgg acgcgccagc aacaacacgc gccagctttt ggaaaaagcc
     4921 ctccgcctcg gagtgcgcgc gcacctggtg gaaaccgctg ccgacctgct gccggagtgg
     4981 ctgcgcggcg cggaaaacat cggcgtcacc gccggcacct ccacgctcga cgagaccgtg
     5041 cgcgcggtga tggaccgcct gcaaaccctg gcctgctgaa ttcccccaaa catcgaaacc
     5101 acaacccgca cacacccatg aacaccggcc attggatccg ccacttcacc cgcaacacac
     5161 ccctcaacga cagcctgcgc ctgcccccgg cccccgtcac gcttcctgat gaaatccggc
     5221 tcccgctcgc cgcgtcactg gccgttttcc agcttggcga atccggcgga ggcacgcgcc
     5281 tgcgccgcta cgcgcgggag gtggctccgc tggtaaactt ccgcggctat cagagggcgg
     5341 tggacttgtt catcgccgag gagcagagtc actcgcgcct gctggagcgc gtggtcctgc
     5401 atctcggagg gtcattgatc gaaaagcagt ggaccaactc ggtcttccgc cgcctgcgcg
     5461 ggctggtgaa tctggagttc gccatccagg tgctgctcac ggcggaactg gtggccgaaa
     5521 tctactacgg tgcgctttat ctgcgggtga gcgatcccgt ggtgcgcgtc atggcgcgga
     5581 aaatcctcca ggatgagacg cgccacctgg agttccagcg agagttcctc tctgaaagac
     5641 tggcgacttt ttcacctgcc gggcgctggc tctggcggct ccaattcgag gcggtccatg
     5701 ccgtcaccgc cgccgtggtg gcctgggatc accgtcattg cctcaaggct ctgggcgtcg
     5761 gtctgcccga gtttttcgag cgcacccgga aagcctgggc tgtctttcag cggcggttgg
     5821 actcccggat tcgggtgctg cgggagtgcg gcggcgtggc gcaggttttg tctccgacaa
     5881 acccgtgagg cgggcggggt ttcgaatgca cactgcgtca aagtgtgtcc accggaatca
     5941 gccgccactc gccctccgcc aagtcaccga gatcatgcgg gccgaagcgt tcgcgatgca
     6001 gcttctccac atgccagccc tggctggcga acatgcgtcg cacctgatga tagcggcctt
     6061 cggtgatcgt gaggcgcgcc gtttgggatg aaacgatctc cagtctggca ggcaggcacg
     6121 gtttgtcctc gccacgcagc atcaaatcac cacgcgcgaa gatttcgacg agctgcggat
     6181 cgagttcgcg gtcgagttcg gccagataaa ccttttccac gtctgctttc ggtgatgtga
     6241 accgatgcac aaggctgccg atgtccgtga cgagcagcag tccgctcgtg tccttgtcga
     6301 gacggcccac gctcgtcacc gccgggttgc ggcgcagcca ttgatccggc agcagttcgt
     6361 aaatcgtcgg cccttcgccg tcgttgtgtg tgcagacgta tcccaccggc ttgtgcagca
     6421 tcgcgagcag gccatcgggt gcttcgagcg gttcgccatc gagtgtgatc gcgtgcggat
     6481 cgactttttg atccgccttg gtcaaaacga tgtcgttttg tttcacgcgc ttcgctttga
     6541 ccaaagccgc cgcctcgcgg cgcgagcagt agccgaggga ggagagcagt tggtcgaggc
     6601 ggcgcatgag aattggcaaa gcagcgcgga tgctccatgc tcaccgcgtc atgtcatcca
     6661 cgatttgcat cattggcggc tgcaacggcg cggggaagtc cacgctggcg cgtgagctgc
     6721 tgccacgcct gggcatcggg cggtttttga acgcggattt gatcgcgaag ggcctctcgc
     6781 cggtgaatcc tgagttggcg gccttctcgg cgggtcgccg gttgctggag gaggcgcaga
     6841 gcttgatcga ggccgggagc agtttcgctc tagaatcgac tttgagcggc aaaacctatg
     6901 tgaaactgct gcgcgaggcg aaggaacgcg gctatcgcct cgtgctgcac tacattgtga
     6961 tcggctccgc tacgcaggca gtggagcgtg tgcggctgcg cgtgctcacc ggcggccacc
     7021 acgtgccgga ggacgacatc cgccgccgtt tcgagcgaag tgtgaggcat attttggagg
     7081 attatctgcc gcttgccgat gagtggggct tgtgggacaa tgcctatgtg cctgcgcggc
     7141 agatcgcaga caacagttcg cataccctcc aacaagttcg cgacatgatc accagcacca
     7201 acctccagga aacacctccg atgccatcca ctgagatgtc acagatcgtg ctcgaagcca
     7261 gccgcgtggc cacggcgaag atgctcgacc tgtatgagcg catgggcatc aaggtgacgc
     7321 cggagatgac gctggcgccc gattcaccac cgccggcatt caagccgttc gagttttggt
     7381 gaatccgctt tctttggcat tgtccggcac gcattgagca cagacttgat ggcgggaaga
     7441 aggcgtcgag ggccgcgttt cagcccgaag ccatgaaaac gacgccattc gtgctctgcc
     7501 ttgctcttgc cgccatgctg cgcgccgcgc caccgcctga cgagccggtg gaaatcggca
     7561 caacgccgca gtttttcgtg gacgatcatc tggtggacaa ccgctgggcc ttgaagcaga
     7621 agcgcgagat ggtgctgcgt gtgtttcatg cgccggtgaa gcacgcgggc aatccgctgc
     7681 tctcgggaga gacgggattt tgctccgtgg tgaaggacga gcagtctggc atgttccgca
     7741 tgtggtacca aacgcacgcc tggaccggca aaggtccgga ggacgagtcg aagacacaat
     7801 acggcatcgc ctacgcggaa tcgaaagacg gcgtgaagtg ggagaagccg aagctcggcc
     7861 tgcacgagtg gctgggcaca aaggagaaca acatcgtcta taaaggcttc atgaactcgc
     7921 gcgccagcgg cccgcagatc ctgcgactgc cggaggcgga ccggcgcggc ttcaaatatg
     7981 tgctcaccta ccgcaccagc ggcgcgaaac gcggcaacaa cggcgtccgt gttgtcggat
     8041 cgcaggatgg cattcattgg gacgagaaga gcgacacgct catctgccag ttccccagcg
     8101 acacgctcaa cagcatcgtt tatgacacca cgcggaagga gtatgtgatg ttctgccgcg
     8161 cgaaaaaccg ctaccgcatc ttcgagggtg acatcctcga caccggcgaa tcccgacgca
     8221 tcgcccgcgt cagccatccg ggcctgtggg aggagtggaa acccgtgccg caggcgattc
     8281 tgacgcccga cgagctcgac gcgcgcgaga acttcaacgc cttctacggc atgccggtgc
     8341 gctatcacgc gggcatctac tggggcgcgc tgtggtgctt ccgtttcaac gacaacatct
     8401 tcaccgagcc cgtcttcagc cgcgacggct tcgatttcga ccgcctgccg gaccgtccga
     8461 aaatactccc actcggcgag gagggctcat gggacgacgg catgaccttc ggcagcatgg
     8521 attgggtcga gatgggtgat gagtggtggt attactactc cggctgggac ggcgatcatg
     8581 gcggcaagga gcgcaacgcc gccatcggtc tcgcgagagg ccgcaaagaa ggcctcgtct
     8641 cgctgcgggg tccgcgtggc ggcggcgtgg tcgtcacacg cctgctcaag tggcccggcg
     8701 gcaaactcat gctcaacgcc gacgccgcga agggcgagct caaggtgcgt gtgagcgacg
     8761 ccacgcgaaa agtcatcccc ggctttgatt acaccgactg cgagcccttc accggtgact
     8821 ccaccgcgct cgagatgaaa tggcgcgggc aatcgctcga cgcgctggcc ggaaaagagc
     8881 tgcgattcga gtttttcctg cgcgaggccg atttgttcac cttccgcgcg gatacatcga
     8941 agtgagccgg gacgttgtca tggctgaacc cggccccaac catgaaaacg cgcctcattc
     9001 tgtgcctgct ggccgccgcg acggctgcca gcgcggagga ggcgttggac atcgcgaaac
     9061 gcagcctgcc gttcatcaaa gaaaaaggtc tcgcgtggat tgaggaccgc cagtgcgcga
     9121 gctgccatca gattccatcc atgctgtgga gcctgaacag cgcattgaag gccggtttga
     9181 tcaccgaaag tccggaagtg gtgaagtgga caccgtgggc cgctgattgg cggcattgga
     9241 accaaaccaa ggacaaagac ggcgtggaca aggtttccgc cggaaacatc gacacgatgg
     9301 tgtttctgct tcttggcagc agcgatccag caccggcctg ggcggcggat tttcgcgcgc
     9361 aactcctcaa aaaccagcag ccggatggct cgtggaaggc tggtgggcag ctccctctcg
     9421 gcaaacggcc accgcgtgag atccatgagg tcacaacgat gtggacgttg ctcgcgctga
     9481 agccgacggc ggatgatccg gtgcgcaaac gtgccgagga ttacctcgcg aaggcgcagc
     9541 ccggtcagag cactgaatgg catgccgtgc gtctgctgct gaagccggac gatgcagccc
     9601 tgcgcgaggc tttgctcgcc tttcagcacg aagatggcgg ctggggctgg ctgacgaacg
     9661 agcccagtga cgcatttggc accggactcg cgctttacgt tctggcgaag agcggactgc
     9721 ctgcagatca cgattcgcga aagaaggcag tcgccttttt aaagagcact cagaggcccg
     9781 acggctcctg gcccgttccg agcacgcggg cgcgggacaa aaacaagatc aacaaaaccg
     9841 ccacctactg gggcacggcg tgggcagtga tcggattgct ggaagatcca gccgtgacag
     9901 gcgtttccgc agcccgctag gctcctttat gaaacaggcg ctcttcttcc tcctcacact
     9961 cgcgcttcac tcacaggcgc agattggcgt gcagggcacc gttggcgcgc ctgcgggagc
    10021 gaaggtggtc aaccggctgg agattacgaa gcctggagtt tacgagaacc tcatcatcga
    10081 cggcaacttc gcgcggggga acctggtgaa gatcacggcg gacaatgtga cggtgcggaa
    10141 ctgcgagatc cggcacagcg cgggcaatgg tatcggcatt ttcgggaaca aggtcgtcat
    10201 cgaaaactgc cgcatccatc atctgctcaa cggcaccttc gaagaccagc aggacgcgca
    10261 cggcatctcc ggccgctggg gcgacacgat catccgcaac tgcgacatct cctttccctc
    10321 cggtgattgc atccagtttg accccgaccg caaatccacc ggcaaggtcg tgatcgagca
    10381 atgcacgctc tggaccgcgc cgctggacaa ggacatggcg ggattcaagg ccggacagcg
    10441 tcccggcgag aacgcgatgg acacaaaaac gatgccggat ggcccgcgct gccagcttct
    10501 catccgcaac tgccacctgc acggctggaa ccagccggcg cagatcgaca acgtggccgc
    10561 gctgaatctg aaggagaacg tcgatgccga ggtgagcggc tgcgtcttcc aaaacaacga
    10621 gatcgcgctg cgcgtgcgcg gtccaggcaa gcgcggcggc gcgcatgtca tcgcaacaga
    10681 ttgcgccatc tacgacacgc agaccggcat ccgcgccgag gatatgatcg aggtgctgaa
    10741 gctcacgaag atcggtttcg gcagcgacat cgggaaacgc atgcagttcg tcggcgggaa
    10801 gtctgattca ggcatccaga tcactggcga gcaagacgcg ccagctgtgg atgggctgtt
    10861 gaaaaagggt tttccggagc gttgagtggc gcatggcttg gagattcgac aaatgcgtca
    10921 tcaatggcga gatcgataac accgtgcgcg gtcgcgtgac gggcaaactg tggctgctgg
    10981 gccgcgagga gccgatgatc ctcgatctcg tcggcgatgc gtggccggac ctggccggat
    11041 gcaggctgac ctttgtgaac acgaatcccg tgccgcagcc gcaatatgac gagggcctgt
    11101 cgatgaggca gaccggttcc gtgggcgaca tcaccgcgtc gatgaagctc aaaaagctgc
    11161 tcgtgccgga ggaggaatgg atgaaggcga tcgaagagaa acgctttcac gaggtgccgt
    11221 gggtcctttc cagctccctc tacctggaat ggttcagtga ccgcaacggg cgcgtggtga
    11281 tccagaccac ggactacaag ctggaggtct ccgagcgcca gtgggaagcg gacgcggatg
    11341 accaggccgc ccagcaggcc atcaacgacg aaaacatgcg cgcctatatg gaagacctgg
    11401 gccgtgcgtt tggcgggcag gaggacgggc aggaggagcg gaaagagggc gaagacaggg
    11461 actgattccc tgtgctccga cggccgcgcg gcacgtcatt attttgggtg aggccatacg
    11521 caactctcag ggactgatcc acacctcggc accctcggtc acgagatcga agagttcgac
    11581 gacatccgcg ttgcgcaggc gcacgcaacc gtggctggcc gggcgtccga tggagcgctc
    11641 gtcgttggtg ccgtggatgt agatgtagcg ctggaacgtg ttggcattgc gtttttccag
    11701 cccgtgcagc cggagaatgc gcgtgagcac gagatcctcg cgcgtatcga gtcccggctg
    11761 ccactgtccg acgggcttgc gcgctttaaa gatggttccg gaaggcgcgc cgccgccgtg
    11821 cttttcacgg atgacaaagc gccccaacgg tgtgcggttg ctgccttcgg cgaagccaat
    11881 tccaaacttc gaggtgctgc acggccattg tttcagcagg cgcgcgccct cgtgcaaggc
    11941 cagtgtctgg ctgccgatgc tgacttcgat tcgtggtgac gcggcggaca tgcccgcatg
    12001 attcgcgcga tccaggcgca ggcaagcgat gcttgccatg cacttgcatc attcacgctt
    12061 gaattccgca ttccatgcgt ctggacatgc ccgcaaagga aacgcgccgc tccgcgtttc
    12121 tcatgaccct ctatgattca tcacccgctt ttccccctca gtcttctggc ctgcatggcc
    12181 ttgtccgccg ccgccgagcc tgtgcgcctg gcgcagacgc cggttgtgtc gccggatggc
    12241 tccctcgtgg cgttttcctg gcggggggac atctggacgg tgccggcgaa aggcggcagc
    12301 gcgcgccggc tcaccacgca tcccgcgctg gacacgacgc cggtcttctc gcctgacggc
    12361 ggccagattg cttttctcag cgagcgcgag ggcggccgcc aggtccatgt catgccggcg
    12421 cgcggcgggc agcccaggca gttgaccttt cacaccgagg gctacgacct ggccgcctgg
    12481 atgccggatg gcagggaact gctcgtcacc ctcgtgcgcg accactcgtg gatgcgcggc
    12541 agccgctccg cgcgcatggc gctgctggat gtcagcgagc gcaaggccga gcgcgtgctg
    12601 ctggatgacg ccgccgcgga gtgctctgtc tcgcccgatg gaaaaaaaat cctctttgtc
    12661 agggaagggg agatgtggtg gcggcagggc taccggggcg cgcgcgcggg ccagatctgg
    12721 ctgcttgacc gcgggagcgg cgagtgcagg caggtcaaag ccgaggccac tgagtgccgc
    12781 tttccgctgt ggaagccgga cggctccggt ttttacttca ccagcaaccg cgacggcgcg
    12841 ttcaacctct gcgagcacga cctcgcctca ggcaggacgc tccagatcac acgcttcaag
    12901 accgactcgg tcgtctttcc cgcgctttcc aaggacggcg gcacgctcgt cttccgccac
    12961 ctggccgatc tttaccgctg gcatcccggc caggacaaag ccccggaaaa aatccgcgtc
    13021 aatcatactg gcgacgcgcc tgacgcgccc gtgcaacgcc gcgtgctgga gcgcgccacc
    13081 ggcatcgccc acaccgccaa cggactccag atggccttca tcgccgacgg ggacgtgtgg
    13141 gtgatggaca ccgagctgcg cgagccgcgc caggtcacac gcacacccga ggaggagcgc
    13201 gacgtggttt tcgcacccga cggcaaagcc ctttggttcg tcagcgacag tggcggccag
    13261 gcggacatct ggaaggccct gcctgccgcc cccgccaagg catggtggga gaacacggag
    13321 ttcaaactgg agcgcgtgac gagtgatccc gccgtcgaaa gcgccccgcg cttcagcccg
    13381 gacggcaaac gcctggcctg gctgaaagag cgcggcgact tctggctggc cgacgccgac
    13441 ggcaaaaatg cgcggcgcgt cttcgcctcg tggaacaagc cctccttcga cttctccccc
    13501 gacggcaaat ggatcgtcta ttcgcagagt gatgaatggt tcaatagcga catctggctt
    13561 ctgccgctcg acggctccaa gccccccttc aacctctccc gccatcccaa caacgactac
    13621 tcgcccgtct ggtcgcccga cggcaaaatg atcgcctgga ccggcgcgcg cgagattgac
    13681 gaggtggaca tcttttatgt ctggctgcgc gcccaggatg acgagcagtc gaagcgcgag
    13741 cgcaccgcca tcaaggcgcg tgaaaaactg gccaaagcct ccgccgctcc cgcggccaag
    13801 tcacccgcga ccaccccgcc cgccaaaccc gccgaaccac ccgccccggc tcctgccaag
    13861 cccggccagg cagaccccaa agccgctgcg aaagcggagc cgcaggccga gtccaaagcc
    13921 gccgccaaat ccaagactgc cgaaacgccc aagcccaagg cgccgaccgc gcccgcgact
    13981 ttgcagatgg acctggagga catccacgag cgcatccgcc gcatctccat tccgaacacc
    14041 agcgagtccg gcctcacctg gtcccatgat tcgaaaaaac tggccttcac cgccaccgtg
    14101 gagggcaagc gcggcaccta caccgtcgaa ttccccgatg atctcaaacc caagatgctc
    14161 tcctccacca cgctgaccga cgcgcgctgg atcaaggaag gcaaccaaat tctcggactc
    14221 aacgaaacca acaccccctc cgccatctca agcaccggat ccctgaccac ctggcgtttc
    14281 cgcgcgctcc agagcaccga caccgccgga cgccagcgcg ccgtttttga ccagtgctgg
    14341 cgcatcatgc gcgaccacta ttacgatgaa aaacttggca accgcgactg ggacgccgtg
    14401 cgcgccaaat actccggcct tgccgcggaa gcgcccgaca tgcgctccgt gcaggagatc
    14461 gttcacctca tgcttggcga gctgaacggc tcccacctcg gcttttccct caacaccgcc
    14521 gccgccagct ccggcacctg gcgggaggag accgcccatc tcggcctgcg cttcgatgac
    14581 aagcacccgg gccccggctg gaaagtgcgc gatgtcctgc ccaacggccc cgccagccac
    14641 cgcctgcgcc gcattctccc cggcgagatc atcctgcgca ttgacggccg cgagacgcgc
    14701 cccggccagg acagcagcga gctgctcaac ggcccactgg agcgcgacat caccctgcgc
    14761 gcgcgcgccg ccgacggcaa ggagcgcgac gtgaccctgc gccccatttc cttctccacc
    14821 gcgcgcggcc tgctctacga gcactggatc aaggacaacc gccgccgtgt cgagcagctc
    14881 tcaggcggca agctgggtta cctgcacatc agcgccatgg acagcgccag ctttcacaag
    14941 ttccaggccg agctttatgc agccggcgcg ggcagggacg gtctcatcat tgacgtgcgg
    15001 gaaaacggcg gcggctccac caccgaccac ctgctcaccg ccctcaccca gccgcgccac
    15061 gcgctgacca tcccgcgcgg cggcagcgcg cccggctacc cgcaggaccg caccatctac
    15121 gccacctggg acaagcccgt cgcggtgctc tgcaatcaga acagctacag caacgccgag
    15181 atcttcagcc acgccatccg cgtgctcggc cgcggccagg tggtcggcgt gcccaccgcg
    15241 ggcggtgtca tcagcaccgg cagcgcccgc gtcatggaag cgggcaccct gcgcctgccc
    15301 ttccgcggct ggtacggcat cgagagcggt ctcgacatgg agctcaacgg cgcggagccg
    15361 caccacatcc tctggccact gccgggtgat gccgcgcgcg gcgtggaccg gcagttggaa
    15421 aaagccgtcg aggttctcaa gagcgatgtc gaagcctggg ccacgcgtcc ccgcccgaag
    15481 ctgctcaaag ccagcgagcg caagttttga tccgccagcg cgcccgctgc ggtgaagcca
    15541 aaatcgcgcc agcatttccc cttgcccggc gcgcggcatt ctcaatgatg gccgcccatc
    15601 atgagcaaag cccccatcac tgtcgcagtc accggcgccg ccggccagat cggttactcc
    15661 ctcctcttcc gcatcgcctc cggcgccctc ttcggcccgg accagccggt gaatctccgc
    15721 ctcatcgaga tcgagcccgc catgggcgcg ctcggcggcg tgtgcatgga gctggacgac
    15781 tgcgccttcc ccctgctcaa cagcatcacc cccacctgcg acctcgacga gggcttcaaa
    15841 aacacgaact gggccatcct cgtcggctcc gtcccgcgca aagccggcat ggagcgcaag
    15901 gacttgctca atatcaacgg caagatcttc accggccagg gcaaggccat cgagaaaaac
    15961 tccgcgtccg acgtgcgtgt gctcgttgtc ggcaacccct gcaacacgaa ctgtctcatc
    16021 gccatgaaca acgccaaagg cgtgcccgcc gagcgctggt tcgccatgac gcgcctcgat
    16081 gaaaaccgcg ccaagagcca gctcgccgcc aaatccggcg cgcaccacag cagcatcacc
    16141 aacatgacca tctggggcaa ccacagcgcc acccagtatc ccgactttta caacgccaaa
    16201 atcggcggca agcccgccac cgaagtcatc agcgaccatg aatggctcca gggggagttc
    16261 atcgccaccg tgcagcagcg cggcgccgcc atcatcaagg cgcgcggcct ttcctccgcc
    16321 gcctccgccg ccaacgccgc ctgcgacacc gttcacagcc tcacccgtcc ggcagccgcg
    16381 ggagactgga cgagcgtcgc cgtgtgcagt gacggcagct acggcatcga aaagggcctc
    16441 atgttctcct tccccatccg caccgacggc gcccggtggg aggtcgtcca gggcgtgccc
    16501 gtcaacgagt tcagccaggc gaagatcaag gccaccgagg acgagctcaa agaggagcgc
    16561 gacgccgtgc gcgagctcgg tctgatctga cagaccccga gctgttcccg gcctttcccg
    16621 gagacggcag ccggggcgcg caggtcaaaa atgcgcgtcc cgattcatga caagtcatgc
    16681 gaccggggtt gcccgcgccg ctctgccgca ttatccaggc gcatgaaatc cgcaccccgt
    16741 cttgctgccg ccattctcgg cgcggcctgc ctctcaggac tccaggccgc gcccaacatt
    16801 ttgtttgtcg tcgccgacga cctcaactgc gccatcggcc cgtatggtga caagtccgcc
    16861 gtcaccccaa acctcgaccg cctcgccgcg cgcgggctgg tctttgagcg cgcctactgc
    16921 cagcaggccg tctgcaaccc ttcccgcgcc tccttcctca ccgggttgag gccggccacc
    16981 accggcgtgg atgacctgag gaaacacttc cgcgacaccg cccccggtgg caaaaaactc
    17041 gtcaccctgc cccagcactt caaaaaccac ggctggttct gccagaacat cggcaagatg
    17101 tttcataaca tgggcgacac ccaggaccgc cagtcctggt ccatggatga gacgatgcac
    17161 accggcaccc acgccgccga caccgtgttc gcgaacaccc ctctcaagcc cggcgatccc
    17221 gcgccggaat acaaagcccc cgtcaccgag gcgccagacg cgcctgacac cgcctaccgc
    17281 gacggccaga tcgcgcgcct cgccgccggt gtcatccgcg accgcgcagg cagcgcgcag
    17341 cccttcttcc tcgccgtcgg attctggcgt ccgcatctgc cattcgtcgc gccaaagaaa
    17401 tactgggacc tccacgaccc cgccgccctg ccttccgctg agcctcccgc gcctccgcgg
    17461 gacgcgcccg ccatcgccct gcacgagtcc cgcgagattc gcggctacgg cggagtcccg
    17521 aaagaccgcc cgttcaccga gaccgaagcc cgccactacc ggcacggtta tcacgcctcc
    17581 atcagctttc tcgacgccca gctcggcctc atcctggacg cccttgagca gagcggccag
    17641 gcggacaaaa cgatcgtcgt tttcacctcc gaccacggct ttcacaccgg cgagcacgcg
    17701 ctttggggca agacctccaa cttcgaactc gacgcccgcg tgccgctcat catcgccgat
    17761 ccccgccagc ctgccgggca tgggaaaaaa acgcgggcac tggccgagct ggtggacctt
    17821 tacccaacac tggccggtct cgccggcgtt gcgcagggtc tccccggcaa cctcgagggt
    17881 gcaagcctcg cccccgtcct cgccgaccct gcggccagcg tgaaggacgc cgcccacacc
    17941 tggcaccagc atccattcta cgggcccgcc agcgcgtggc aggcttgggg atgctccatg
    18001 cgcacccagc gctggcgcta caccgaatgg cgctccattc aaagccgcga agtcatcgcc
    18061 cgcgaactct acgaccacga cagcgacccc ctggagaccg tgaacctcgc ctcccagccc
    18121 gctcatgccg cccgcgtcag cgagctcgcc gggaaactcg ccatccagtt cccgcgctga
    18181 ccccgtggct tagcgcgggt ctgtgccgtg ctcgccgcct gtctcctcga cagggaggcg
    18241 cggcttggat gccagcggcc tgtcctctgc aaaccagcgg ttgttttcaa agcggaaggt
    18301 ctccgccgcc acgcccgggc tcatgttcac atcctcgcgg atgtccgcgc ggcggaagac
    18361 gacgcggttg ccgcgcacga tgcccccgcg gcagggcgtg aaaccctcgg cgcgcgtttc
    18421 ctggaggatg cggaagatcc agcgggcggg ataaagcacg gtgttgtcct cgaagagcgc
    18481 gccgtccacg ccgacaaatg cggcggcgca caggctgccc tcgatgtggt ttccacgcac
    18541 ggtgatgtcc ttggcctcat gtttcgctcc cggcgggcgg aagaacggca tgccggtcga
    18601 gccgccgatg ttcaccgggc gctcgccggc gttttcgaag cggcacttct ccaccaccac
    18661 gcccgagctg ccgcccttga tctgcacacc ggcggtggcg gtgaagccct ccttgccggt
    18721 gaaacggcac ccgctgatga ccacagcgtg gcagccgacg aggtccactc cctgcccgcc
    18781 ccagccggtg atcacgcagt cctggatcac ggctccctcc aggccggagg ctttgatgcc
    18841 gtcgtggttg ccgctggggc cgatgtcgct cacctgcacg ccctcgatcc ggatgccttt
    18901 gaccagcggc ctgccggcgc ctccctcgtc aaggttgatg ccgttcacgc tctggccgcg
    18961 cacatggatg aggcgcagca ccagccccgg gcaggcggag aaatgccagc cgcccctgcc
    19021 gccctcgaac acgggcgggc gctccttgtc cagcgcctcc acggtcagat tctccacgcc
    19081 gctcacatga tgcccgccgg gataaatgcc cggcgcgatt ttgaccacgt ccccggattt
    19141 caagccggcc agcaccgcgc gcaattcccg ctcatcccgc gccacatgcc cggcggcgga
    19201 caatgtcgaa cccagcagga caagagagag aaagcgcgtc atgcccatcc aacgcggcat
    19261 cccggcgttt ccttgtcggt tgccgccggt ttgaggtcat ttgccgtcgt cctgaccggc
    19321 gaacaatggc agccgcacgg tgaaggcggt gcctttgcca gtcgcggact cgacatgcac
    19381 cgagcctccg tggagttcga cgcagcgttt gacgatgacg agtccgaggc cggtgccggg
    19441 tgtttcgccg acgttgctgc agcggtggaa cgcctcgaaa agccggccgc gctcgttttc
    19501 agggattccg atgccgtggt ccacgacctg gaagacggcg tcgccgccct cgcggcgcgc
    19561 ggtgagcagg acttcgccgc cctcggggct gtatttcacg gcgttggtga tgaggttgct
    19621 gaaaatgtgg cgcagcaggg attcgtcggc gcgcgctcca tcgagcccgc cctccgagcg
    19681 ccagatgacg gggcattttc ggttcgtggc ggagaggttc tcgtccacga ttttgccggc
    19741 gaggatgtcc aggtcgcacg gcgcggtttt gcagccgagc ttgcccgcct cgacacggcc
    19801 aagcacgagc acctgctcca tgaggccggc catgtggcgc gttgaggtga agatgtcgtt
    19861 ttgcagctcc agacgctgat cgggcggcag cctgtcgttg tagtggcgca tgagttcgat
    19921 ggcgctcatg atgatgccca gcggcgtgcg gaactcatgg ctgaccatgg tgacgaagcg
    19981 cgacttcaaa tctcccagct cgcgctcctg cgccagggcg cgcgtgatct cggcctgggc
    20041 tttttcaagt tccgcggtgc gctcggcgac gcgcttttcc aggagggcgg cgaccctggt
    20101 cttcactcgg ttggatttgc gcagcgccgc gatccacccg ctgaacaggg caatgacggc
    20161 cagcgtgccg ccgccccaca accaaagctg gcgcgcgcga accacggcgg agacaccgag
    20221 ggacctcacg tcggcgtcat caagcaggcg catctggcgc gggctgaggg cggtggcgcg
    20281 ttcgacgagg ccgcgcacga gcacgtcgtc atccggcttg atcgtgtcga aggcggggcg
    20341 gccaccggcg cgaaacgcga cgggcatgcg cgcgccgtcc gcctcgatca tcagggtgct
    20401 gcgccattcg ccattctcca gcggcacttg atgaagggtg acgagccggc cgcgcgtctc
    20461 cacccagtcg gaggtgcgct ccccggcggc gatttccgcc agcgcggcgg tgacgggttg
    20521 cggaggttct ttgcggcccg tgaggcgcag gacgcactcc aacagatcgc agcctggggg
    20581 cgttgtttct ccgaatctcg catcatgcga aaaaccggtg atctccacct cgtcgcccgc
    20641 ggcgggagct gtctccctgg cacgggcgcc cgtcctttcc tcaccgctcc acggctgcct
    20701 gagggcggcg cgcaatgcgg tgccatcgtc ccggatgtgg atcgcgccgt cgcttgtgcg
    20761 tccgagtacg atgccgcgca cgcgcgcagg ccggcagccg gcattcgctt cggaggaaac
    20821 ctgtgacgcg gggatggtct ccgcttcaaa aggatcgttc acgccagtct ccagcacctc
    20881 gaactggctg atgcccggca cgaagatgag gcggcccagg acgggggcga aggtgaccac
    20941 gcctgtgaga cggagacggg caccgtgaag tttatcaggc ggcgcgcccg cgcccggcag
    21001 cgacacaaag ccgtcaaaaa cgccgccatc cagcacaagc cgcagccgca ggtcggttcc
    21061 atccagacgc cagccggcga cgcgcgcctg gcaggagagc cactggtcca tcgcggaatc
    21121 cgagagcagt ttctccaatg tggcaggctc cggcacgggc aggtctccgc tgccggtgat
    21181 gatgaactca tccgccgaga cgcgcatttg ccggagtcct ccaagcatgt tgaccatcgt
    21241 cgagccttcg acctcgaccc ggtctccaag cgccggcggg gcgagccttc gaggatagcg
    21301 cacggcgacg catctcccgc cctcatgcag atagaagaaa ttgaacatgc cgctgacact
    21361 cacgacgacg cctttgacac ggacggcgcg cgccgtctgc cccgtctggc tgacgccgcc
    21421 gtcgagcacc tgcgcgacag tggtcaacgg cgccggctcc tgcccatgcg cggatgctgc
    21481 gaccagcagg gccgcgatga gccgcaggaa tgtcagcggg gaaggtctgg ctttcgtgct
    21541 gctcatgggc gggctaggtt ttcaagtgtt cgttcacggt ggagagcagg tcttcgcgcg
    21601 agactggctt ggtgaggtaa gaatcggctc cgagaccgag gccctcgctc acgtcggact
    21661 tgtcgccttt ggcggtgaga aagacgaagg gcgtgccgcg cagcgtctcg tcctcatgca
    21721 gggctcggag cacggcatga ccgtccatct cgggcatcat gacatcgcac aagatgagat
    21781 caggccgctc ccggcgggcg gtttcgatgc cttcgatgcc gttggcggct gtgagggcgg
    21841 tgtggccttc gagctggagc atgagcgcca cattgcggcg catggtggcc tggtcttcga
    21901 tgatgaggat ttttcgcggc atgggaggat cagttgagga tgggaatgcg gacggtgaaa
    21961 gtgctgccga ggccgggttc gctggcgacc tcgatgctgc cgccatgcag ctcgacgcag
    22021 cgtttgacga tgaccaggcc gaggccggtg ccgggcgtgt cgccgacatt ggagcagcgg
    22081 tggaaggcct cgaagagccg cgcgcggtcc ttctccggga tgccgatgcc gcggtcgctg
    22141 acttcgaaga cggcctgcgg cccgcggcag cgcacccgca gggtcacttc ggatccttcg
    22201 ggcgagtatt tcacggcgtt ggcgacgagg ttggaaagga tgtggcggag cagggactcg
    22261 tccacatttg aggaagcggg cactgcctcc gtgtccagcg tgacgcggca tttgccgctc
    22321 gtgatggact gggtctcgtc aatgacgcgc tccgcaaagg ccgccagttc caggggagcg
    22381 ggcttgcagg tgagtttgcc ggcctcggcg cgaccgagca gcagcacctg ctccatgagg
    22441 ccgcccatgt gtttggtgct ggactggatt tcctggagct ggcgtttttt ttcatcctca
    22501 gggaggcggt cggagtagtg ctgcaggagt tcgacggcgc tcatgatgat gcccagcggc
    22561 gtgcggaact catggctgac catggtgacg aagcgcgact tcaactcgcc cagctcgcgc
    22621 tcctgctcaa gcgcgcgccg cagctccgac tgcgcgcgct ggagctcccc ggtgcgctca
    22681 ttgacgcgtt tttcgagcgt ctggttgatc tccagcatcg cctgctcggc gcgcttcatc
    22741 tccgtgatgt cgtggtggat cacgacgacc tcggagattt ccccggcctc gtttttcagc
    22801 ggataaagca gcccgccgat ccagcgcgtc tccggagggt cgccgggaac gggaaaaggc
    22861 accggaggca cctgcaccac ctcgccctca aaagccctgc ggatgtggtt caccgcgccg
    22921 gactcgatga gatcgggcgc ctggagcacg ttgaaggcga ggccctcctc gtcgctgagg
    22981 cggaagagcc tccgccaggc gtcattgaag agcttcgtcc gcccgtcggg cgcgaagatt
    23041 tgcacgctca gcgggctttg ctcgaaaagc agcttccagc gcctctcgct agcctgcgcg
    23101 gcatctttgg cggtcttgag ttcggcggtc atccgcgcct gcctgagctg ggtgcgccgg
    23161 agcagcagca cccagccgcc gagaatgccg aagactgcca gggcgatgcc gccccagagc
    23221 cggaggcggc gcgtgacgac ttcgaaagac acgccgagag actccacatc cgacggcttc
    23281 aatatccaca acagccgcgg gctggtggag gtggcgcggt ccacgaccgc ctgaatgatg
    23341 acatcatccc cctcgttcag ggtttcgaaa gaatgcggcg cggcgctctg atgcaccgcc
    23401 gtcatgcgcc ggccttcgga ggccaggagc agggtggctc gccatttgcc ttccttctcg
    23461 cgggcggctt gcaggctcag cagccgtgcg cgcactttca ccagatccga tgtgtgcccg
    23521 cctgcggcga tccgggccag cgtggtctcc accggctcga tgttttcacg atgccccgtc
    23581 acgcgcacgc tggcgctggc caggtcgaaa ggctcgtacc tttccgtggt gaactggtgt
    23641 cccgagactt ccagctcgtc gccaattttc aaagcaggca gggggccgca gtcggcatac
    23701 gcgatgcccg gcggattgcc cggccgcgcc cagggttgct ggaggctgac gcacagggac
    23761 gcgtcttcgc cccggatgta aagcaccgtc ctctcctgcc ccacgagcac gcctttcgtg
    23821 ctcacaggct cccccttggg cgcgcgtccg gcggctattt cggaggccgg gtggaagggc
    23881 gcgccgaagg ggctgtccct gcccggtttg accaattcca caaaggcggg gccgggcacg
    23941 atcatgtccg ctccgcggaa attgatcgcc aaccccgtgg ccaggccggt gaagcgcaga
    24001 cgcgcgccga agagattttt ggggaatgcc gccaggtcgg ggctgcgcac actcacggtc
    24061 gtccaggtac ggtcgtcacc cgcggcataa gtcatcgcgc tggcttgcag catccaggca
    24121 tgaaccaccg cttcgaaagt cacccagcgc gcctcgtcgc ggtaagccag gaaggtctcg
    24181 agtgagactt cctccggctc cggaaccgcg cccctgccaa cgacgcgggc atgacagcgg
    24241 accatgccga ggaatttcgc gttgtaatcg gtttgatcca cgacttcgcc cgccgcttcg
    24301 atgacatccc ccggctgcat gggggggagc gggacgcccc ttccggcaac ctcgggtgaa
    24361 gctgaagccg ggtcgccggc gaggacagcc gcggcgccgc tgccgtcatg aagtatcagc
    24421 gtgcgggatt ccatgatcgt gatgagtgcc cccctcaccc gaaccctgcg gtccagtccc
    24481 gtcttccgcg cgttcaggtc cttgatcgtc accagaggcg cggcaaaaat gtcatccggc
    24541 atggtctggg cggaggctgc ggaggccgtc acaatcagca gcagtgcccg caggcattgc
    24601 tgtccccaaa tgagctggcg gattattttc atcaatttcc agcccgaacc tagcacggaa
    24661 ccgcgcgcgg tggcatccga ctaaagtcgg atgccaccgc ctttgcattc cagccggggc
    24721 gggcttagca tccgcagccg cacccctgac caacgcggcc gcctccgtgt gaccttcgcc
    24781 acccagatca ccatcttccg cattttgctg attccagtct tcgtcggcac ggcggtctat
    24841 tacgggcaga gcgtggccgc gggcgctccg aacgaatccc tgcgcgcctg gaccatcgcc
    24901 gtcttcgccc tcgccgccgt gagcgacgcg gtggacggct atgtggcccg ccattacaac
    24961 cagcgcagcc gccttggcgc cattcttgat cccctggctg acaaactgct gctgctctcc
    25021 gccattgtga ccttgagctt caccggctgg cgccagcagt ttcccctgtg gttccccacg
    25081 ctggtcatct tccgcgatct ggccagcatc ggcggcgctt tcctcatcca gcatctggcc
    25141 gggagctgcc acatccagcc gcactggacg ggaaaagtgg ccaccttcgc ccagatcgcc
    25201 gccgtgctct ggttgatgct ggagtttccg ttcctgctct ggccgaccgt ggtggctgga
    25261 gtgttcaccg cgctttccgg cttcatcaac ctggcctccg gcatccgcca ggtgcgccat
    25321 gtggggtgag ccgcgcccgg atgcggcatt gacaggagct tgaaagagcg cacggagggg
    25381 aaaaccgcgc gcgcactcgc gccaccaccc ccttgtccca tccatgaaaa ccgtcgccat
    25441 cgtcggccgg cccaatgtcg gcaagtccgc cctgttcaac aggcttgcgg gcatgaacat
    25501 ctccatcgtc catgacatgg ccggggtgac gcgcgaccgg atcaccgccg agtgccgccg
    25561 ggggagggag gtcttcgaga tcatggacac gggcggcatc ggcgcgaaca ccagcgacat
    25621 cctcaccgag caggtgcaga cggaggcctc catcgccctg gaggtggcgg atctgctgct
    25681 ctttgtcgtg gacgtgcatg acggcgtgac gccgatagac caggccctgg cgcaggtgct
    25741 gcgccgcgtc aacaagccgg tcgtgctcgt catcaacaag gccgactccg acaagcgccg
    25801 gctcggctcc ggcgagttct cgcggctagg cttccccgac tccgcggagg tcagcgcggt
    25861 gcatgggctg ggcatcaagg agcttgtcga actggtttct gagaaactcg gactggaaga
    25921 cgaggagacc gaggaagtcg tcgagcaaaa gcgcgccggt aggccgttga agctcgccat
    25981 cgtcggcagg ccgaacgcgg gcaagtcctc cctcgtcaac gccatcctcg gccaggagcg
    26041 cgccatcgtc agcgaggtgc cgggcaccac gcgcgacagc ctcgacatcc attgcgagtt
    26101 caacggccgc cgctaccagc tcatagacac cgccggcatc cgcaggcagg ccaggctgga
    26161 agaggccgtc gagattttca gcgtccagcg ctcttaccag gccatcaaaa gggcggacat
    26221 ctgcctgctc gtcatagact gcgccggcgg cgcgaagatg caggaccgaa aagtggccca
    26281 gctcatcctc gaggagcgca agccctgcat cctggtgatg aacaaatacg acctctacca
    26341 tccctccgcc aaacaaaagg accgcatcga ggagctggag gagaccatgc ggcgggagtt
    26401 tttctttttg aattacgcgc ccctcgtcgc catctccgcg aagaaaagag agcacatcgg
    26461 caagctcttt gtgcagatcg aggccgtacg caggggggcg caaacgcgca tcggcacagg
    26521 cgcgctcaac cgtctgctgc acgaagccat cgccaacaca cccggccccc tggggcgcag
    26581 ctcgcacgcc tttaaactcc tctacgccac ccaggtcaac agcccggagg aggagcaggt
    26641 gccggtgccg cacttcgtgc ttttcgccaa ccgcgccgcc aagctcacag acagctacct
    26701 gcgtttcctg gagagcgtca tccgcaagga atggcccgcg cccggagtgc ccttccgcat
    26761 gagcgtgcgc ggcaaggagc cgaaggaaaa aggcaggcgc tgatccagcc tcctctcact
    26821 gctccgactc ggcaacggtg acgctgatga cttcctcaat ggtggtgagg ccggcgagaa
    26881 tcttgcgcac gccgtactcg cgcatggggc ggtagccgtc ttttagcgcc tgctggagaa
    26941 gttgctggcc gctggccttg tgggtgatga gctcgcccat cgccggggtg aggcgcacga
    27001 gttcgtaaat ggacatgcgg cccttgtagc cggtggcgcg gcaggcctcg cagccgcctt
    27061 cgcgcgcgcg atagaccggc gcgccgacag ggccgtggta tttgagctcg tgcagcttgg
    27121 cctgggcgtc gggctcgatc tccttgcagt ccgagcagag aacgcgcacg aggcgctggg
    27181 cgatgaaggc gcgcacggag ctggccacca ggaagggctc cacgcccatg tccacgaggc
    27241 gggtgatgcc gccgatggcg tcattcgtgt ggagggtgga aaagaccagg tgcccggtga
    27301 gcgcggcgcg gatggcgatc tcggcggtct ccaggtcgcg catttccccg accatgacca
    27361 cgttcgggtc gccgcgcagg atgctgcgca ggccggtggc gaagctgagg ttgatctccg
    27421 gccgcacggc gatctggacg actccgggca gcttgttttc caccggatcc tcgatggtga
    27481 cgatgcggcg gtgctcggtg ttgagctggc tgaggaaggt gtagagcgtg gtggacttgc
    27541 ccgagccggt ggggccggtg acgaggatga tgccgttggg ctttttcagc agggtctcga
    27601 cctcggcgcg caggtcggac tcgatgcgca ggcggtcgag gttgaacttc tcctggccga
    27661 gaagacgcag ggagacgctc tccccctcga cgctggggat ggtggccacg cgcacgtcta
    27721 tgaactggcc gccgacctgg aggttgatgc gcccgtcctg cggcaggcgg cgctcggcga
    27781 tgtcgagctt ggccatgact ttgaggcggg cgatgacgga ctgctgcagg gccttgatgt
    27841 tttccggcac gggcacctcg cgcaggcggc cgtctatgcg gtagcgcatg cgcagcttgt
    27901 cctccatggg ctcgacgtgg atgtcggtgg cgtgctgctc gagcgcctcg cggatgacgg
    27961 tgttgacgaa cttgaccacg gaggcctcct cgtcgtcgtc gtcaatgacg ttggcctcgt
    28021 cccgctgctc gaggtcggcg tcgtccacgc tgcggccttt gaggatttcc tcaaaggtgt
    28081 ccgcgcccac gccatagaag tcgcgcatgg cgttgagcag gcgcttgcgc ggggcgatct
    28141 cccagcgcac gggcgcgtcc acctcgcgcg cgacggcctg acgggcgatg aggtcgaagg
    28201 ggtcgtaggc ggcgatgacg agtttgcggt cgcagccctc ggcggcgggt tcctggattt
    28261 tgagcggcag cagccggtgc ttcaaagcca cgcgcggcgg cagcagccgt ttcatttcag
    28321 gcgcgtcggc gctgtcgggc agagggttcg gcacgaagcc caggccgatg cgcgcggcga
    28381 gctgggagag aaaggcctcc tcgtctattt tgccggtgtc gagcagttga ttgaccaccg
    28441 aggcgctggg cgtggccgtg ggcagggcga tgcccacgcc gccggacgct tcaaggcatt
    28501 gcacgagcgc ctggcgcacg ccgctggagc gct
//
LOCUS       DAOMYJ010000042        28477 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Prosthecobacter sp. isolate AMR_MDS_5629
            NODE_589_length_28477_cov_22.818134, whole genome shotgun sequence.
ACCESSION   DAOMYJ010000042 DAOMYJ010000000
VERSION     DAOMYJ010000042.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867850
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prosthecobacter sp. (wastewater metagenome)
  ORGANISM  Prosthecobacter sp.
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Verrucomicrobiaceae; Prosthecobacter.
REFERENCE   1  (bases 1 to 28477)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 28477)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.30
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:34:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,742
            CDSs (total)                      :: 4,684
            Genes (coding)                    :: 4,676
            CDSs (with protein)               :: 4,676
            Genes (RNA)                       :: 58
            tRNAs                             :: 55
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28477
                     /organism="Prosthecobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_589_length_28477_cov_22.818134"
                     /isolate="AMR_MDS_5629"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1965333"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2197
                     /locus_tag="PLP58_06615"
     CDS             <1..2197
                     /locus_tag="PLP58_06615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="WD40 repeat domain-containing protein"
                     /protein_id="HRJ08223.1"
                     /translation="IEARPVPLIEQAMMWAARRPALTGMAAALLVVLAGASALLLRAN
                     RDLKESLHAEAAALDTSEERLQTALMAEATALLRSHELTAREETLKVVIELVASGRRD
                     VETRTLAARALAMPGIGAGQMLQTGYAKGSSSTAITPDFSRHAVGGGSQGKYFEIVSP
                     SGSQKRVVVRTPAVPNNFRFSADGRWLAVHFRDHSVQVHSVAEPEKPVFHLPRGGRRW
                     GVALDFHPKEAWWLYADGSPALRRHDAATTGNGKDPVVFNAPGGVIGLTLAPDGTRVC
                     VQWSGGWGVVELASGRLLWQRAETSTLAWPVWHPSGKTVGNGVENPYRFVLVDAATGH
                     ETTAFYGHQGAISHAVLHPQQPLLFTLGWDRQLIMWDAWSGQAMGHLPAMVRGLAVRR
                     DGAEMAFAPEYLGTSRYEFAVPRVWRTWEAPLIPGVSVIGLDVSLDGRWLVSNSPGMA
                     RLWEISTRRHVADVPMQGARSNRTSFWLEDGMLLHAVDGVAGSIASGHRLDVRPMLRE
                     NGGSFDPKETAVTDGGPRQRMTDRRGLVLRRADTSGAVLDPDGSTRLPFGPWEVMEIS
                     PDGEWAAGEGSQQGEYVVRRVADGGIVLSITIKPWVKLAFSPDSRHLVTAGSTKVIVH
                     ECGSWREVARWPVSIGFQGMGWVEFSPDGRHLATLHAEGTLALHETAGWTPVVHLRPP
                     GPIFQHSGAARFQWTADSRRLLVLTQGHRISEWDITALREDLGKLGLDW"
     gene            2426..8107
                     /locus_tag="PLP58_06620"
     CDS             2426..8107
                     /locus_tag="PLP58_06620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ08224.1"
                     /translation="MMKTLALSLLLPVCLLMFTQSALAQTSPVISITVTPPAGTAAAP
                     LFVIDSDSGTAPQTRNTIAATVTVTMQRSTAATSAVFRMQGDLLPSSSSTPVSLISSS
                     RNSAVQTIDLKGTGTVTRTFTLNLKPNEALGAGAAFRVRAQLQVETTTKVFSNVGSSV
                     TSGSFTVVHYRDSTTSTNLNVHAHLSAAATISKGFALTTNASQRDFEVRVPLLITRYD
                     LGDGARPINLRTIVTLEDDLGAQIPLASNANVLTTLSSLLASNTGTPNTPATRSLILT
                     RSFRPVAQLDSVNRTYRVRVKVEIQDDAVNTTALRDLGNLVNLSNQRLLHFNGTLRFT
                     GGAVATMTAISNTPTAGALEPNPPSKVSLATNRIRTVLSLSGGALTGQAGMTFGSITN
                     LDARLAPDGTAEVVAMPVTTVNQPPGIELSGTFGRIKVSYASPRLTSAGLVVDAVINL
                     PQGLGFTGERATSRNKLLRLLPAVSAVTLSSDLRHTGTLNGSITGADAWVFDEARPLW
                     FRVTKFFFATNGELSFANPAAEWAHKGAFKQLNDQQIRGEHQVPTMADRLTNDGWLKS
                     VEATSGTVAFTAATDGTARTTGVKLTLGEGEVRPHFPSAPAGSPDLLLEWISGNAEIE
                     NGKIKAGSTLTGAQSFTVAHESACQKEGCPQPAPSLRTVTCSPEEGIFLLTPDGGLAC
                     ATDFGAPFALAWDRRENAEEFSHQTGKFSAATFFMPGHQSYVAEASLPDARAPGALLN
                     AGLFGQTLVYPHTRAYLEGRGLYAGLNFTVTSGAEVVGEIRVADMTSGIELPLRPQVS
                     KYYARRSGVSGRHVAQAAPDALTLYGYPFGFTKMEMAFLSNSMDGVAEPSRVNGEVQV
                     PKVKEQTETEFTQKFTGLKIACLGALGGAEIDPTDRSAKPLSYWNGSISPKAIQFVPE
                     NPLLGDCAAGTRLLAMLVESSVANIEEPLFGSLVFRNTGNLTGLQPASLGMDGRLGLP
                     GVVKMRGPRKGGSDAEPEFETYEMWPMSKLYFNDALDARAPDTGFVTFGALCNVPFYE
                     DLEVQVLTSANHADTAAPFHLASGWRENGLTYANSLFFDSAHRGLPPADQNVTFLQYR
                     NPAPNSPDFRIVAKQSIFGILDIEDKLTWNALTRDFSADPTQKLPLLVLSLEHELLYL
                     SAERAELTFGAQFADLKHLNLANAAVEGVERLHTAAHVRVNQGARRLRRGIDRLDDLV
                     EDRLDQWIEQYSEGITTAIFNKLHRAFEDSYNAHVINGPLGYPEWVDRQNGKLKDVVN
                     QALEQGGIEGVTQPLREQLRRLTDNLEGSNSLLGEVKDALDQAILTLDIVAGGFLVDG
                     QDNPLNTVFTRQPDGSLSPTIEGENARLGFISENPETGQREIASNLVRELVKASLPPD
                     LAHLADAIFALTGAPTVADQALNELIAEADPALDSITEVLQQVKGVLVDTRNQVAAVQ
                     GLGEELQRVVDASEARLNSIVNEVRDSIFTFFDEAAERAVGGPLDAGQKVIGLFQEFS
                     EKTLSDLLKATLRDALMESGIVQDIQHLLRQRLAELRHHLHDAIDQIYARLSKITKKI
                     AKKFLAPIDEQVNKILGKLPDVLGAGSVDGYADIVGDTLRRLRLDAKVELSLPEKMQL
                     EAFFEMSCFDSSTDLEATQGCPAANGDKTVEVRVAALDIPLSWTPSVEPSADTVAEAA
                     GGANPNAEENNDELRASLGIWFGFAGGEPNSIGGSFEMTSGEFDFESVVVSDVRAAVA
                     IGFEQAYLAAGVRVKVSEYQGSGGVFFGRTCSIEPLRLVDPDAAAVLGDPPFTGAYVA
                     GEVWVPISEVAFGVPATCMFTVSAGVGAGVFYFADGPVFGGRLDLGISGEALCAVTFS
                     ADLGMVGVLTPTGFNYRGVVTVSARVGPCPLCAPFDRSFPVNYKDGNWSFE"
     gene            8140..11619
                     /locus_tag="PLP58_06625"
     CDS             8140..11619
                     /locus_tag="PLP58_06625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ08225.1"
                     /translation="MKHLPLILACFLAAVTLRAQETLLTLGTTTTVAGQPHAYVLWQP
                     QASEDSLFKKFGIYSKPGDAQSAGVFVREGIQTLQASPRTIRAMLELGSKLDLDPLGA
                     ARRIDGMFRDLTLGHGQAPAAPGNLDASEKLAYIIQASTTNPRLLTQLFFLGRAHAGV
                     MLALGHAFTHPMPAGVRTYEIRELDENDADMRVLGRVTLNPLAPVLIFNPDRPVAVRH
                     LPKANSPHPASSKDHLNVRLRWSAPANLRSTYAHSFGFDVYRVKKGVAESLGWHLQRP
                     AAADVLNLLAAMNPASPDPDIARANELPVLVNRLLSTDEANDLNDDQTVYFADDGVRH
                     DAPDGRRVLRPYSDGESFYYFAAARDITGRPGIVSTGLLVTICDRLPPLPPRIENVFS
                     TFEAPAEGAVGQTGSQFLRVKFRQLPENQQEGATKYFVYRWSTPQEVIQHAGDVGFNR
                     VGTLTHTPGATFAVFDDKGAGAPNVTTHLDETVWYTLRAVGRTACGDEKTLSAHSAPV
                     PGVLRDFKAPDAPQGGITVVQNTPTIQPAGNPVFFEDKGASNAALGQALQARGLDLGF
                     RGLLVEITRQSSEIASAHLVVEVQQADNSFLPVYSASTSFQSGDVVRVPLPFRLEGSR
                     RIRLKARAITKNGIFPPPAEVTLSKPASISLVGLSEVRFTASSTRRELPLGGLVNAHE
                     VANADGTVNPVRGTLVYAAPEIREWRIYRRVHPDGDLALIQKSEGNPLESTATGKKIA
                     AGFWENDSLPSTPGTTVCYYAQIFDQNANPSPLTLLGCVPLKGAPLPTPMLTEVNTVA
                     SGKDNQPEFVLEWFCDPVGVERFEVLIAQEGGGEPQVVGVVPVQLDPVPFTPADLEGG
                     NPNAGLLTMPGRSDLRAHGFQTGPVSSASMGSGPRFAATLVVTDPLPRSLVIAVRAVG
                     AGRAPREAGAASNAITVNTDFTIGTIGGDPAGVIPWPARPLPPVIGDMREESYGTREG
                     PYLAIPSGNAGPVDAATLILAGEVPAGLLVDASASGEQTDLSKSVGAPESLFWRHRPV
                     REDAGTTEPLMPCIIYRHQLPSEAFPNARANLVQCTPLLDRLSWQDLTSPSVRRIRDP
                     FICIFEKAAVTGNSKVAQLPAKTPYLDDADAAIFIKDPLPVIAGARYQHLIVRFDDKG
                     EISRVIACPPVQH"
     gene            11640..23426
                     /locus_tag="PLP58_06630"
     CDS             11640..23426
                     /locus_tag="PLP58_06630"
                     /inference="COORDINATES: protein motif:HMM:NF012200.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choice-of-anchor D domain-containing protein"
                     /protein_id="HRJ08226.1"
                     /translation="MKHAARFLLIFALVCATARAAPTSKLPSEWFVPLDTDSGRFALV
                     DRATGTIRFGIMNPDGKTGFSQVVPTGITEVSDTASGILGPQGEEIVLTAPAANRVIR
                     VTAAAEFPLAYVFQPLSGLGTSGVSESETAAVRKLLVSSLANAPTNISKLELRDPLTG
                     ALITELNSKELVQRLEPMTNPADGNRVSIGVHTDATGTFIGVSQPPEKREVFSSSQPH
                     EILTDILGSAGGRHFIAFRNGFAAASLFTAALPITKTSSFSSASVNFPFPVSALLRVP
                     GGGDSPLTDGVIALAADGTQAVWLRVNAAGNGFITTPHSFTPDPGSALTGLLPMPQGV
                     VLLSGAQAGGISTDFKGMAFDGTDWVTRSSGSLPALPTAVTAAAHLLFLDRDPVTENG
                     ARVVGMQTVPDWTRRTGSEPLPASVVREIFQGSADGLRFNSTQAVTPPPGTNFVLANQ
                     ADPAMSVVTLGTAASVFTPTLQVEPPSGSYDESFQVTATFDTKAFKLRFRRDGEPWQD
                     WTGPLPVAYTTTLQFALQSLAGGAPGPVQSRSYLFTANQLLAQDSDGDGVPDFVELEK
                     GLDPFGGADHDRDGISDLDELIRGTGAADANDPRGSTKENPGGNAPVLRGTGLRFVAL
                     PFDVLGSQMNPNQPVQLRTLTGGLLAEANTAVFDPQLPDNSIFGARLSTSASVPVEEL
                     VALSSPDSFTTFNGAAGREIIRFVPADPPPQLSVSITPTGANLTGDANAWINAARAAV
                     AAVPFAAARTRLDPPDMAVSVLLEHLVHSALVSVRSVENPAPSLEDFSLFSQRPSDGG
                     RLRLNEEDKVLLITQGFLFRKALTQSQIRRTAMSALANGIYARHSAASNPPSLDLPLD
                     ILRVVLRGEDLPPEYAGAATSGQIATARTAYNGAVASALGNFRPDTIIEVEIPPTPLG
                     EGIYLGDFGGELEPVALFRLGRRFLLDDRFGSQPGTRFSIRAFTDLPPDRGRVALDVI
                     SAEFLSSPPNLDRDSDLNGLDDEWERFFFGSIGQNPGFIPPGHTKTLAQLFANGEDPR
                     ALAVEPDLLVEQPAGNVLAPLAGVNFGVMGLNSSQNLVFTVRNTGRSDLTGLGISFSG
                     DQAGSFSVTLEPLTPVTPQAQSTFTVRFRPSTAGSHSAVMSIASNDPDENPFQVQLSG
                     TGVIVGHPHPGFSPVIGSANIIALATQPDGKILAGGLLTSVNGQAVGNLARFNADGSL
                     DTSFNTGTGAGSSVICLTVQADGKILVGGNFTTFNGATATRIVRLNSNGSTDSSFSAA
                     FSGGVGTVTGIVTQPDGRLIVVGNYTSANGQARNRIVRLLPDGTLDGSFNPGTGADDN
                     IRNAVVQADGRIVIAGDFANFNGQPRNRLARLNADGSLESTAGFNPGTGPNGLVRCLS
                     LLPDGQIMIGGVFTTVNGFPRSGLAVLSSSGTVGAFTCQGITQVFSFIRQTDGDVIVG
                     GGFTTINSQARNRIARITPATLGSAAFVPDTTFQVGIGANNTVNAVALAANGGVIAGG
                     LFTTMNGLPRSGLAVLVNESPATNITVTADNRILWSRSGTAPEIAEAEFDVSADAGAT
                     FTPLGTAQFIAGGWQMNEVDLPDEGLIRVRAAVRGGQGNSSAGLVQTSAPFSLNTTST
                     PVISSIAAVTTDEDVSAGPIAFTVSDTHTSAERLQVTAVSSNSTLLPASGIALGGAGG
                     QRTITLTPALNQSGSATVTVRAFDGARTGTRSFTFTAQPVNDAPGISPFADHVTALNS
                     PVVISFAVSDVETPAESLIVTGTSSNTAVVPLDGIVLTGGSKREVRIIHAPNAVGSST
                     ITLTVSDGAATAQSSFQVTVNNSAPVIAKKSDITLNEDFFITATPITISDNETPADNL
                     VVTLESSNHALLPPSRLDVFGTGGSRFLSIQPLPDQNGSAVVTVNVSDGLLTTEMSFT
                     VSVTPLNDAPVIAEIADLSTREDTPVTIPISVTDIDGPVIALGAFQVNPPPGAVHGVT
                     VNQADRTVLITPPPDFHGTATFTVTVNDSRLSDATTFTLTVLPVNDAPSFTKGPDFAH
                     PFGFNTPQTVENWATDLSAGPANEASQNLSFRVLSVSQPAGLTEPFFAAAPTLSPQGR
                     LNYRTRGLGGISTIRLVIEDDGGTENGGVDRGVEQTFTITVGDAQPVLVIETENGQAL
                     EEGVGVLNVGSVTPGGSVRRQLTVRNAGQAPLNFGGVVKGGGLERFIVGFVQTGVLLP
                     GESRQVQIAFAPDTPGVHSGVLTFLSNDPVEARQNYSITLSGSGASAPVVRLLRPNSV
                     TRQSVDLSAVVNAGNADTTVTVDLGTTIDFGITVPAQPSIVSGGDETPVRAVASGLLG
                     GTLYFYRVRAQNAAGVSTAQGVFFTETNTAGMLDESFLPQVDANGRVLALAVQMDGKI
                     WVGGDFIEINGTPCAALARLNPDGSLDKIFNLRNEGSEVLPEVRAIVVRPDGRVVVGG
                     VFNRINDTACHLLVRLNSDGTLDDDFANVTFGTGPADSVLRLLLQTDGSLIVGGRFSS
                     VNGAPRTSLARLRDDGALDTTFAPVLGGGGPLTPEVLSMASQDDGRILIGGLFATVNG
                     APRVNVARLGEDGTLDAGFDARMEFHQGLGEVNSILVKPDGGILLGGAQLRFLDRSPG
                     INDFLAALREDGSAGEGVALDIEQALSSSSQGAGPLPSRLNVPTLTDLAAQADGSVVM
                     AGNFTLISNGRLVNNIARILPDGTLDADFQADVPVRALECLAIQGDGRVLAGGFFTQV
                     NGVAQFNLARLLNDAPFESLEVVDGSRVEWRRGGAAPEARELLLDISTDGGLSFAPLG
                     EAVRIPGGWALVDAGLAELDVFHIRARTHLISGGGNGSAGIMQSVIAHNQPDIEVLTL
                     DKQEFVDDVSTHDFGPSLLTGLVTQTFVIRNAGPLPLQIGAIAVDGLAAADYAVNTTG
                     VSAILENGQITTFTITFAPKDTGFREAVLRIASNDPDAESSFDVDLLGEGVPPGPPQM
                     VVRGPNGEAFGHRAKLELGSVLKGQTAGFVFTIENQGFAPLTGLAVSKINGGNGFTFT
                     PVGSASISRGQSTTFLVSFKPPNSTPQGMRLSLASNDPAQTPFEFDLRAAEAVRSVAV
                     DFNGTRLKSGAASLQNLGSNLTFGVPVQRTFNLVNTGNVAFVIANLVVNHPDYQVSAP
                     GKVQLAPGGDSTPFTVTFTPRSGGLSHASVQVLGNEGPQPFFDLRLQGGIQAQEIAVE
                     QPAGASLASGSSVVDFGTPLAGGRVVRVFTLRNTGTNTLNLGAPVFEGGAPFTLTKLP
                     PGALVAGKSVTFEVTFAPAAGGDFAGNLLLPNNDLDENPFRIELRGSALPSLLPVITG
                     HPQSQIVPLGAAVSFDGGAASETALSFQWQRGLGALSNFGKALATSPLRIAKVTLNDA
                     SNYRFQAINAFGSTASDSARLVVVDTKVSAFNLTPGRALALRLNVAGPGPLELLWHKD
                     GAPLTASQRVTGVTGQDLVINNLTTADSGIYTCVVTTPQGVLTGGENRVNVVELAPQI
                     TLNPAVMPDGRIGLPYSFFIPLNPERGLATTFNAAGLPAGLVLNAATGLISGNPTQSR
                     TQPFSVTLKATNSKGSSTLVATISVAPLPEGLAGVYAGIIERAAMNRHLGGRFDLAVA
                     GTGSFTGKVLLGAGIQDNLQGVLRLSPGSPLVSVEILTGGRAYTFTLNPQSRRITDGV
                     AASGEGTADFIGWREAFNESSGAGLAFAGSHNFGMEMPSTVRGKPGFPEGTGFGHCNV
                     RNGGRDAVITGKTADGEACTGGSVLSDNGEVVVFSRGTKGSLSGVMKVLPDLAPPFGN
                     IRLGGELSWMRLPLAGRVYPAGFGPLNLRIFGGRYREPAFATVLGSPTARLTFAGADI
                     GGPPSLVDRLFTLPFGVQSFTLKDRDVTLNVRPGANARLESGGFSGSFKFTQRDVANP
                     PFNVLTRSASFEGVIIPDGEFQRGVGFFNLPSMPQRGQTATQTPILSGSVQLDPVR"
     gene            23632..26193
                     /locus_tag="PLP58_06635"
     CDS             23632..26193
                     /locus_tag="PLP58_06635"
                     /inference="COORDINATES: protein motif:HMM:TIGR02603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HRJ08227.1"
                     /translation="MPKASAKKKVNRPQAVGPAIGANVATPVSNIKTLKDFKVELIYS
                     VPGGEQGSWVNLTTDDAGRIYVSDQYGMLYRFKAPAAGQALSQGDIEKVPADIRGVNG
                     MLFAFDALYVGVNDYEKKIPSGVYRITDSNSDDMPDKVEMLREFDAGSDHGVHAILKT
                     PDGKGLYLISGNNAEIKEGPKAGTPDSSPVAKLWGDDHLLPRMPDGRGHNRHVMAPGG
                     IVYRFTPDGKTFEIFASGFRNIYDGGVNRDGELFVYDADMEYDFNTSWYRPTRINHVV
                     SGAEFGWRNGAGKYPEFYIDNLPATLNIGPGSPTGCTFGYGAKFPAKYQEAFYALDWS
                     WGKIYAVHLTPNGSTYTATKEEFVTGGPLPVSDAIIGQDGAMYFTIGGRRVQSGLYRV
                     SYVGKESTAPSDKSDRSDESDKAVALRRSLEAFHGKQDPKAVATAWPHLSSTDRYIRA
                     ATRTALEHQPLAEWQDKALAETNVTAQLEALLALTRRTGVCPTHRTDPSYQPDSKSRD
                     AILTALLKHDFAKLTNEQKLGYMRIIEIVLHRFGNPDDATVAAIIAKLDPAYPADNFE
                     LNWLLTETLAHLQAPNTAAKAMALIAEAGSQEPEIEYARSLRFLKAGWTPELRTQQLE
                     WFLKAASYKGGSSFTKFIEFIRNDSLATFTEAEKATHAALIEKKPEIKSAIENVGAIF
                     VGRTPTMWTLEELSAAAQTGMKGRDFDTGRKMFSAAACYTCHRFGNAGGMTGPDLTGS
                     GGRYSPHDLLDQIINPSKEINEQFAPIIVTKNSGEILSGVVVNLSGDNVTLNTDLSDP
                     DQRVNIDRKEVKSIELSKVSPMPPMLLAMLKKDEILDLLAYILSGGDKGHAMFVK"
     gene            26261..27352
                     /locus_tag="PLP58_06640"
     CDS             26261..27352
                     /locus_tag="PLP58_06640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018969377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRJ08228.1"
                     /translation="MVHISIREVSKRFGRQTVLDRVSLEIEAGELFFLLGPSGCGKTT
                     LLRHVAGFYQPDEGRIFFDEDDVTRLPVHKRDTGMMFQSYALWPHLNVAQNVAFGLEE
                     RRRPQAEIEHRVNEALELVQLGGLGVRRIAQLSGGQQQRVALARALVVHPRCLLLDEP
                     LSNLDAKLRHEMRAEIRRICKQFGLTGIYVTHDRDEALSMADRLAVMDSGRLAQVGTP
                     EEVYRNPASRMVAGFIGGTNFIEAVVAAPAGADGMHEVHTVFGALRARSNSASWRPGA
                     GVKVLLSVRPEALAFAAAGAGENSIPARITGATYLGATVQYDMRVDGGPDLHACETNP
                     RLVRQPSEARISLTVAAEDVVMLPVETRH"
     gene            27357..>28477
                     /locus_tag="PLP58_06645"
     CDS             27357..>28477
                     /locus_tag="PLP58_06645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009958167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HRJ08229.1"
                     /translation="MTSMSPVIATLAELVRINSVNSSYEGGPGEGEIAAHVRRFFEKH
                     GIEVREQEVFPGRPNVIARLPGRNPARRVVLEAHTDTVSVQGMTIPPFEPVIRDGKMY
                     GRGSCDTKAGLAAMMHALASLKEEGVTPPCEVLLAAAVDEEYSYRGVVRFCEGLRADA
                     AIVAEPTELRAVIATKGVLRCRIVAHGKAAHSSKPHLGVNAITAMARVIGALETDAGR
                     FAQNRHPLTGQATLNVGVIHGGVQVNFVPDRCAIEIDRRLLPGEQAETALREYEELVA
                     KVCAALPGVRAEIEQPPLLVDEALDTPADSAVVRTARAVLDEMGLDSAPCGVPFGCDA
                     SKLSRAGIPAIVFGPGSIDRAHTADEFIELDQVERALEF"
BASE COUNT         5151 a         9054 c         8952 g         5320 t
ORIGIN      
        1 catcgaggcg cggcccgtgc cgctcatcga gcaggcgatg atgtgggcgg cgcgccgccc
       61 ggcgctgacg ggcatggcgg cggcgctgct ggtggtgctg gcgggagctt ccgcgctgct
      121 gctgcgtgcc aaccgagatt tgaaggaatc cctgcacgct gaggcagccg ctttggacac
      181 ctcggaggag cgtctgcaaa cagccctgat ggctgaggcc acggcgctgc tccgcagcca
      241 cgagctgacc gcgcgcgagg agacgctgaa ggtggtgatc gagcttgtgg cctccggccg
      301 gcgcgacgtg gaaacacgca cgctggcggc gcgggcgctg gccatgccgg gcatcggcgc
      361 ggggcagatg ctgcagacgg gttacgcgaa aggcagcagc agcacggcca tcacgccgga
      421 tttcagccgc catgccgtgg ggggtggctc ccagggcaaa tactttgaga tcgtgtcgcc
      481 gtccggctcg caaaagcggg tcgtggtgcg caccccggcc gtgccgaaca attttcgctt
      541 cagcgcggac gggcgctggc tggcagtgca ttttcgggat cactccgtgc aggtgcattc
      601 cgtggcggag ccggagaagc cggtctttca ccttccgcgc ggcggcaggc gctggggcgt
      661 ggcgttggat tttcatccca aggaagcgtg gtggctgtat gcggatggca gtccagccct
      721 gcgcaggcat gacgcggcca cgacgggcaa tggcaaggat ccggtggtgt ttaacgcgcc
      781 ggggggcgtg atcggcctga cgctggcacc ggacggcacc cgcgtgtgtg tgcagtggtc
      841 aggaggctgg ggcgtggtgg agctggcgtc cgggcggctg ctgtggcagc gcgcggagac
      901 ctccacgctg gcctggccgg tctggcatcc ctcggggaag acggtgggaa acggcgtgga
      961 gaacccctac cgctttgtgt tggtggacgc ggccacagga cacgagacga cggcctttta
     1021 cggacaccag ggggccatca gccacgcagt gttgcatccg cagcagccgc tgcttttcac
     1081 actgggttgg gaccggcagt tgatcatgtg ggatgcgtgg tctggccagg ccatggggca
     1141 tctgcccgcc atggtgcgcg gtctggcggt ccgtcgcgat ggcgccgaga tggcctttgc
     1201 cccggagtat ctgggcacct ctcgatacga gttcgctgtc ccgcgcgtgt ggcgcacctg
     1261 ggaggcgccg ctgattccag gagtcagcgt catcgggctg gatgtgagcc tggacggacg
     1321 ctggctggtg tccaactccc ctggcatggc ccggctgtgg gagatcagca cgcgccggca
     1381 tgtggcagac gtgccgatgc agggcgcacg ctcaaatcgc acgagcttct ggctggagga
     1441 cggcatgctg ctgcacgcgg tggatggcgt ggcaggctcc atagcgtccg ggcatcgcct
     1501 ggatgtgcgc ccgatgctgc gggagaatgg agggagcttt gatcccaagg aaaccgccgt
     1561 gacggatggc ggcccgcgcc agcgcatgac cgaccgccgg gggctggtct tgcgccgagc
     1621 ggacacatcg ggagccgttc ttgatcccga tggcagcacg cggctgcctt tcggcccgtg
     1681 ggaggtgatg gaaatctccc cggacggtga atgggcggcg ggcgagggca gccagcaggg
     1741 cgagtatgtc gtgcgccgtg tggcggatgg cggcatcgtt ctgagcatca ccatcaagcc
     1801 ctgggtgaaa ttggcctttt cccccgacag ccgtcatttg gtgacggccg gcagcacgaa
     1861 ggtcatcgtg catgaatgcg gctcctggcg ggaggtggca cgctggccgg tgagcatcgg
     1921 tttccagggc atgggctggg tggagttttc cccggatggc cgccacctgg ccacgctgca
     1981 cgccgagggc accctggccc tgcatgagac ggcgggctgg acacccgtgg tgcatctgcg
     2041 cccgccgggg ccgatcttcc agcacagcgg cgccgcccgg ttccagtgga cggcagacag
     2101 ccgccggctg ctcgtcctca cccaaggcca ccgcatctcc gaatgggaca tcaccgcgct
     2161 gcgggaggat ctggggaagc tggggctgga ttggtaagca gccagccagc tttttgaggc
     2221 aggcatagtt ttccggggaa aatctcaata ctttgtgaaa cctctctcga ggaatccgga
     2281 agatagtagg gagatgaaga ctgaactccg cttgtccttg ctctcctttt cccggatgcc
     2341 cgctcttttg cgcggctttg agcgcaagtt tttccacgaa gtgcatccac cctcggctgg
     2401 ctggctgcca gcacatcctt cttccatgat gaaaaccctg gcattgtccc ttctgcttcc
     2461 tgtctgcctg ctgatgttca cccagagtgc gctggcgcag accagccctg tcatctccat
     2521 cacggtgaca cctcctgccg gcactgccgc ggctccactc tttgtgattg attcggacag
     2581 cggcaccgcg ccgcagacgc gcaacacgat cgcagccaca gtgacggtca ccatgcagcg
     2641 gagtaccgca gccacctcgg cggtttttcg catgcaggga gatttgctgc catccagcag
     2701 ctctacgccg gtgagcttga tctcctcatc ccgtaactct gccgttcaga cgattgatct
     2761 caagggaacc ggaacagtga cacgcacctt cacgcttaat ttgaagccga atgaggcgct
     2821 tggcgcaggc gcggccttcc gtgtacgcgc gcagcttcag gtggaaacca ccacgaaagt
     2881 cttctccaat gttggcagct ccgtgacgag tggcagcttc actgtcgtgc attaccggga
     2941 ttcgacgacc tcgacgaacc tcaatgtcca tgcgcatctc tctgctgcgg ccacgatcag
     3001 caagggcttt gccctgacga ccaatgcctc gcagcgcgac ttcgaggtgc gcgtgccttt
     3061 gctcatcacc cgctatgacc tgggcgatgg cgcgcgtcct atcaatctgc gcaccattgt
     3121 cacccttgag gatgacctgg gtgcccagat tccgctggcc tcaaacgcga atgtccttac
     3181 cacgctgtct tcacttttgg catccaacac gggcactccc aacactcctg cgacaagaag
     3241 cctcatccta actcgcagtt tccgccccgt cgcgcagctc gattccgtga accgaactta
     3301 ccgggtgcgt gtgaaggtag aaatccagga cgacgcggtg aacacgaccg ctctgcgcga
     3361 cctgggaaat ctggtgaatc tctccaacca gcgcctgctg catttcaatg gcacgctgcg
     3421 cttcaccggc ggggcagtgg ccaccatgac tgccatcagc aacacaccga cggcaggcgc
     3481 gttggagccc aatccgccca gcaaggtcag ccttgcgacc aaccgtatcc gtaccgtgct
     3541 cagcctcagc ggcggcgcgc tcacagggca ggctggaatg acgtttggct cgatcactaa
     3601 tctcgatgcg cgcctggcac ccgatgggac ggcagaggtg gttgccatgc cggtgacaac
     3661 agtgaaccag cctccaggaa ttgagctttc gggcactttt ggccgcatca aagtgagcta
     3721 tgccagcccc cgcctcacca gcgctggcct ggtggtggat gctgtcatca acctgccgca
     3781 aggccttgga ttcactggag agcgtgccac cagccggaac aaactgctca ggctgctgcc
     3841 cgccgtttcc gcagtgactc tcagctcgga tcttcgacac acgggcaccc tcaatggcag
     3901 catcacgggc gcggacgcct gggtcttcga tgaagcccgc cctctttggt tccgggtcac
     3961 gaaattcttc tttgccacaa atggcgagct gagtttcgcc aatcccgccg ccgaatgggc
     4021 gcataaggga gctttcaaac agctcaacga tcagcaaatt agaggggagc atcaggtgcc
     4081 caccatggct gaccgcctga ccaatgacgg ttggctaaaa tccgtggagg cgaccagcgg
     4141 cacggtggca ttcactgctg caactgacgg cacggcgcgc accaccgggg tgaagctcac
     4201 tcttggagag ggcgaggtgc gtccgcattt tccttccgcc ccggcaggtt cgcctgattt
     4261 gctcctggag tggatctcag gcaacgcgga gatcgagaac ggcaagatca aggcaggctc
     4321 aactctcaca ggtgcgcaga gcttcacagt ggcgcatgag tcagcctgcc agaaagaagg
     4381 ctgtccccag cctgcgccct ctttgcgcac ggttacatgc agcccggagg agggcatttt
     4441 cctgctgaca ccggatggtg gcttggcctg cgccacggac tttggagcac cctttgccct
     4501 ggcttgggac cggcgtgaaa acgcggagga gttcagccat cagaccggca agttcagcgc
     4561 ggccaccttc ttcatgccgg ggcatcaaag ctatgtcgca gaggcaagcc tgccggatgc
     4621 acgcgcgccg ggggccttgc taaacgcggg cctgtttggt caaacactgg tttatccgca
     4681 cacccgcgct tatctggaag gccgtggact ctatgcgggg ctaaacttca ccgtcaccag
     4741 cggcgcggag gtcgttggcg agatcagggt cgcggacatg acatcgggca tcgagcttcc
     4801 cttgcggccg caggtttcca aatactacgc caggcgcagc ggtgtcagcg gccgccatgt
     4861 ggcgcaggcc gcgccggacg cgctcacgct ttacggctat ccattcggtt tcactaaaat
     4921 ggaaatggcc ttcctatcca acagcatgga tggcgtggcg gagccttcgc gcgtcaatgg
     4981 cgaggtccag gtgccgaagg tgaaagagca gacagagact gagttcacgc agaagttcac
     5041 cggactgaaa atcgcctgcc tgggcgcgct cggcggggct gagattgacc ccaccgacag
     5101 gtccgccaag ccactcagct actggaatgg cagcatctcg cccaaggcca tccagtttgt
     5161 gccggagaac cctcttttgg gggactgcgc cgcaggcacc cgcctgcttg ccatgctggt
     5221 ggagagcagc gtggcgaaca ttgaggaacc gctttttgga tcgctcgtct tccgaaacac
     5281 tggcaacctc accggactgc aacccgcaag tctgggtatg gacggtcgcc tgggcttgcc
     5341 tggggtggtg aaaatgcgcg ggccgcgcaa aggcggctcg gacgctgagc cggagttcga
     5401 gacctatgag atgtggccga tgtcgaagct gtatttcaac gacgcgctgg acgcgcgcgc
     5461 tccagacacg ggattcgtga cctttggcgc gctctgcaat gtgccgtttt atgaggacct
     5521 ggaggtacag gtcctcacca gtgccaacca cgccgacacc gcagcgccct tccacctagc
     5581 ctctggctgg agggaaaacg gcctgaccta cgccaacagc ctcttttttg acagcgcgca
     5641 ccgcggcctt ccaccagcgg atcaaaatgt gaccttcctg caataccgga accctgcgcc
     5701 caactcgcct gatttccgca tcgtcgccaa gcagtccatc tttggcatcc tggacattga
     5761 ggacaagctg acatggaacg cgctgacacg cgacttttcg gcagatccca cgcaaaagct
     5821 accgctgctc gtgttgagcc tcgaacatga actcctctac ctcagtgcgg agagggcgga
     5881 gctgaccttt ggcgcgcagt ttgctgatct gaagcacctg aacctggcaa acgccgccgt
     5941 tgagggggtg gaacgcctgc acaccgcggc ccatgtgcgg gtgaaccagg gggcaaggcg
     6001 cctgaggcgc ggcattgacc ggctggatga cttggtcgag gaccgcttgg accagtggat
     6061 tgagcagtat tctgaaggca tcaccacagc catcttcaac aagctgcaca gggcttttga
     6121 ggactcctac aacgctcatg tcatcaatgg tccgctcggt taccctgagt gggtggaccg
     6181 ccagaacggc aagctcaagg atgtggtgaa ccaggctctt gagcaggggg gcattgaggg
     6241 agtcacccag cctttgcgtg agcaactgcg caggctcacc gacaatttgg agggatcgaa
     6301 cagcctgctg ggagaagtta aagacgcgct cgaccaggcc atcctgaccc tggacatcgt
     6361 ggcgggtggt ttccttgtgg acggtcagga caaccccttg aacaccgttt tcacccgcca
     6421 acccgatggc agcctcagcc ccaccatcga aggtgaaaat gcgcgactgg gcttcatttc
     6481 ggagaaccct gagacaggcc agcgcgagat cgccagcaat ctggtgcgcg agttggtcaa
     6541 ggcctcgctg ccgcctgatc tggcgcatct ggcagacgcg atctttgccc tgaccggagc
     6601 tccaaccgtg gctgaccagg ctctgaatga actgatagcg gaggctgacc ccgctctcga
     6661 ctccatcacc gaggtacttc agcaggtaaa aggcgtgctc gtggacacgc gcaaccaggt
     6721 ggccgccgtg caggggcttg gtgaggaact tcagcgtgtc gtagatgcgt ctgaggccag
     6781 gctgaactcc atcgtgaatg aggtgcgaga ctccatcttc acattctttg acgaggcggc
     6841 ggaaagagcc gtgggcgggc cgctggatgc cgggcagaag gtcatcggcc ttttccaaga
     6901 gttcagcgag aagactttga gcgatctttt gaaggccacc ctgcgggacg cgctcatgga
     6961 gtccggcatc gttcaggaca tccagcacct gctgcgccag cgtctggcgg agttgaggca
     7021 tcacttgcac gatgcgattg accaaatata cgcgcgcttg agcaagatca ccaagaagat
     7081 cgccaagaag ttcctggcac cgattgacga gcaggtgaac aaaatcctcg gcaaactgcc
     7141 ggacgtgctt ggcgcgggtt ccgtggacgg ttatgcggac atcgtgggcg acacgctgcg
     7201 acgcctgcgt ctggatgcca aggtggagct gagcctgccg gagaagatgc agcttgaggc
     7261 gttcttcgag atgtcgtgct tcgactccag caccgatctg gaagcgaccc agggctgtcc
     7321 tgccgccaat ggtgataaga cggtggaagt gcgggtggcc gcgctggaca tacctttgtc
     7381 ctggacgcct tcggtggagc cttccgcaga caccgtggcc gaggccgcag gcggggcgaa
     7441 tcccaacgcg gaggaaaaca acgatgaact ccgcgccagt ttgggcatct ggtttggatt
     7501 tgccggaggt gaacccaaca gcattggcgg ctcctttgag atgacgagtg gcgagtttga
     7561 tttcgagagc gtcgtcgtct cggacgttcg cgcggcggtg gccattggtt ttgagcaggc
     7621 ttatctggcc gccggggtga gggtgaaagt ctcggagtac caagggtcag gaggtgtctt
     7681 cttcggccgc acctgctcca tcgagccgtt aaggctggtg gacccggatg cggctgccgt
     7741 gcttggtgat ccacccttta ccggggccta tgtggcaggg gaagtctggg tgcccatttc
     7801 agaagtggcg ttcggtgtgc ccgccacctg catgttcaca gtctccgcag gtgtgggtgc
     7861 gggtgtgttt tattttgccg acggcccggt cttcggcggc aggctggact tgggcatttc
     7921 cggcgaggca ttgtgcgcgg tcaccttttc cgccgacctg ggcatggttg gcgtgctgac
     7981 tccgactggc ttcaattacc gtggagtcgt cacggtgagt gccagggtgg ggccatgccc
     8041 actttgtgcg cctttcgacc gcagcttccc cgtcaactac aaggatggaa actggtcatt
     8101 cgagtgagcc aacaacccaa tcttccagct tacagaacga tgaaacatct ccctctcatc
     8161 ctcgcgtgct tcctcgccgc cgtcaccctc cgcgcgcagg agacgctcct caccctgggc
     8221 accacgacca ccgttgccgg gcagccgcat gcctacgtcc tctggcagcc gcaggccagc
     8281 gaggacagtc ttttcaaaaa gttcggcatc tactccaaac cgggcgatgc gcaaagcgcg
     8341 ggtgtctttg tgcgggaggg catccagact cttcaggcca gcccgcgcac cattcgtgcc
     8401 atgctggagc tggggtcaaa gctcgacctc gatcctctcg gcgcggcccg gcgcattgac
     8461 ggcatgtttc gtgaccttac cctgggtcac ggccaggctc ccgccgcacc tgggaatctc
     8521 gatgcctcgg aaaaactggc ctacatcatt caggcatcca ccacgaaccc gcgcctgctc
     8581 acgcagttgt tcttcctggg ccgtgcccat gctggcgtca tgctcgctct gggccatgcc
     8641 ttcacccacc cgatgcctgc cggagtgcgg acctatgaaa tccgcgagct ggatgaaaac
     8701 gatgccgata tgcgcgtgct gggacgggtg acattgaatc cgctggcgcc ggtgctcatc
     8761 ttcaatcccg accgaccggt ggcggtgcgt caccttccca aggcaaactc gccgcatcca
     8821 gccagctcaa aggaccatct gaatgtccgc ctgcgctgga gcgcgcccgc caatctgcgc
     8881 agcacctatg cgcacagttt cggctttgat gtctatcgtg tgaaaaaagg cgtggccgaa
     8941 agcctgggct ggcacttgca gcggcctgcg gcggcggatg tgctgaacct gctggcggcc
     9001 atgaacccgg ccagcccgga ccctgacatc gcccgcgcca acgagctgcc ggtgctggtg
     9061 aaccgcctgc tcagcaccga tgaagccaat gatctcaatg acgatcagac ggtctatttc
     9121 gccgatgacg gtgtgcgcca tgatgcaccg gatggccgcc gcgtgctgcg cccttattcg
     9181 gatggcgaaa gcttttatta cttcgccgcc gcgcgggaca tcaccggccg ccccggcatt
     9241 gtctccaccg gcctgctggt gacaatctgc gaccggctgc cgccgctgcc gccgcggatt
     9301 gaaaacgtgt tcagcacttt tgaagctccg gcggaaggcg cagtgggcca gaccggcagc
     9361 cagtttttgc gggtgaagtt ccgccagctt cctgaaaacc agcaggaagg ggccaccaag
     9421 tattttgtct atcgctggag cacgccgcag gaggtgatcc agcacgcggg ggatgtgggg
     9481 ttcaaccgcg tgggaacgct gactcacact cccggagcca cttttgccgt gtttgatgac
     9541 aaaggcgcgg gtgcgcccaa cgtcacgacg catctggacg agaccgtttg gtacaccctg
     9601 cgggcagtgg gacgcaccgc ttgcggggac gaaaagacgc tcagcgctca cagtgctccc
     9661 gtgcccggtg tgctgcgaga cttcaaggca cctgacgcgc cccagggtgg gatcaccgtg
     9721 gtgcaaaaca cacccacgat ccaacccgca ggcaacccgg tcttctttga ggacaagggt
     9781 gccagcaatg cggccctggg gcaggcactg caggcacgcg gactagacct tggctttcgg
     9841 ggcctgctgg tggagatcac acggcagtct tcggagatcg cctctgcgca tctcgtggtc
     9901 gaggtccagc aggcagacaa cagtttcctt ccggtataca gtgcctcgac cagctttcaa
     9961 agcggcgatg tcgtgcgtgt gccattgcct ttccgccttg aaggcagccg caggatccgc
    10021 ctcaaagcgc gcgccatcac gaagaatggc atctttcccc cccccgccga ggtcacattg
    10081 agcaagcccg cgagcatcag cctggtgggt ctttccgagg tcaggttcac cgcctcctcc
    10141 acgaggcgtg agctgcccct gggcggtctg gtgaacgcgc atgaggtggc gaacgccgat
    10201 ggcaccgtga atccggtgcg cggcacgctg gtgtatgccg cgccagagat ccgcgagtgg
    10261 cgcatctacc gtcgtgtgca tcccgacggc gacctggcgc tcatccaaaa gagcgagggc
    10321 aatccgctgg agagcactgc cacggggaag aaaattgcgg ccggtttttg ggagaatgac
    10381 agccttccct ccacacctgg taccacggtc tgctactacg cgcagatatt tgaccagaat
    10441 gccaacccca gtccgctgac cctgctgggc tgcgtgccgc tcaagggtgc gccgctgccg
    10501 acacccatgt tgaccgaagt aaacaccgtg gcttcgggca aggacaatca gcccgaattt
    10561 gtgctcgaat ggttttgtga tccggttggg gtggagcgct tcgaggtgct catcgcccag
    10621 gaagggggcg gcgagccgca agtcgtgggg gtggtgccgg tgcagcttga tccggtgccc
    10681 ttcactcctg cggacctgga ggggggcaat cccaatgcgg gcctcctaac gatgcccggc
    10741 agatcggatc tgcgtgccca tggctttcag accggacctg tgagttcggc aagcatgggt
    10801 tccggaccac gctttgccgc gactttagtc gttaccgacc cgctgccgcg ttcgctggtg
    10861 attgccgtgc gtgccgtggg tgcaggcagg gcaccgcgcg aggcgggggc ggccagcaat
    10921 gccatcactg tgaacacgga cttcaccata ggcaccatcg gtggcgaccc ggcgggcgtc
    10981 attccctggc cagcgcggcc tttgccgccg gtgatcggcg acatgcgtga ggaaagctat
    11041 ggcacgcgcg agggaccgta tctggcgatt cccagcggta acgctggacc cgtggatgct
    11101 gccacactca tccttgcagg cgaggtgccc gcgggcctgc tcgtcgatgc cagtgcatca
    11161 ggggagcaaa cggatctctc gaagagtgtc ggcgcaccgg agtcgttgtt ctggcgtcat
    11221 cgtcccgtgc gcgaggatgc gggaaccacg gagccgttga tgccttgcat catctaccgc
    11281 catcaactgc ccagcgaggc ctttcccaat gccagggcca acctggtgca atgcacgccg
    11341 ctgctggacc gcctttcctg gcaagacctt accagcccat cggtgcggcg catccgcgat
    11401 cccttcatct gcatcttcga aaaggctgcg gtgactggaa actccaaggt cgcccaactc
    11461 ccggccaaga cgccttacct cgacgatgcc gacgcagcca ttttcatcaa ggatccgctg
    11521 ccggtgatcg cgggtgcccg ctaccagcat ctcatcgtcc gctttgacga caaaggcgag
    11581 atcagccgcg tcatcgcctg cccgcccgtc cagcactgaa gctcccactc cattctgcca
    11641 tgaaacatgc agcccgcttt ctcctgatct tcgcgctggt ctgtgccacc gcgcgtgccg
    11701 cacccacctc gaagctaccc tccgagtggt ttgtgccgct cgacacggac agcggccgct
    11761 ttgccctggt ggacagggcc actggcacca tccgcttcgg catcatgaac ccggatggca
    11821 agacaggctt ctcccaggtg gtgcccacag gcatcactga ggtcagtgat accgcctccg
    11881 gtatcctcgg cccgcagggg gaggagatcg tgctcactgc gcctgccgcc aaccgcgtga
    11941 tccgcgtcac ggcggcggcg gagtttcctc tggcttatgt cttccagcca ctcagtgggt
    12001 tgggcacttc cggcgtgagt gagtcggaaa ccgctgcggt gagaaaactg ctcgtctcct
    12061 ctttggcgaa tgcgcccacg aacatctcca agctggaact gcgggatcca ctgaccggcg
    12121 ctctcattac cgaactgaat agcaaagagc ttgtacagcg gctggagccg atgaccaatc
    12181 ccgccgatgg caatcgtgtc agcatcggtg ttcacacaga cgccacgggc accttcatcg
    12241 gcgtcagtca gccaccagaa aagcgtgagg tcttttcatc cagccagccg cacgagatcc
    12301 tcacggatat tctcggcagc gcgggcgggc ggcatttcat cgcctttcgc aatggcttcg
    12361 ccgcggccag cctcttcaca gcggccttgc cgatcaccaa gacctcgtcg ttcagttcgg
    12421 cgtccgtcaa tttccccttt cccgtgagcg cgttgctgcg tgtgccaggc ggcggggaca
    12481 gtccgctcac ggatggcgtc atcgcgctgg cggcggatgg cacgcaggcg gtgtggctgc
    12541 gggtcaacgc ggcggggaat ggcttcatca ccacgccgca cagtttcacg cctgatcccg
    12601 gctccgctct caccggcctg ctgccgatgc cgcagggcgt ggtgctgctc agtggagcgc
    12661 aggcgggcgg catctccacg gattttaaag gcatggcctt cgatggcacg gactgggtga
    12721 cgcgcagcag cggctccctg cccgcactgc caacggcggt cacagcggcg gctcatctgc
    12781 tgttcctgga ccgcgatccg gtgaccgaaa acggtgcgcg cgtggtgggc atgcaaaccg
    12841 tgccggactg gacacgccga actggctccg agcctctgcc cgcatccgtg gtgcgggaga
    12901 ttttccaggg cagcgctgat ggactgcgtt tcaacagcac ccaggcggtg acaccaccac
    12961 ccggcaccaa ttttgtcctg gccaaccagg ctgatccagc catgagcgtc gtcaccctcg
    13021 gtacggcggc ttcggttttc acacccaccc tgcaggtgga gccgccgtca gggtcgtatg
    13081 acgagagttt ccaggtcact gccacctttg acaccaaggc ctttaaactc cgcttccgcc
    13141 gcgatggtga accctggcag gattggactg ggccgctgcc ggtggcttac acaacgacgc
    13201 tccagtttgc cctgcaaagc ctcgctggcg gcgcgccggg acccgtgcag tcgcgcagct
    13261 atcttttcac cgcgaaccaa ctgctggctc aggatagcga tggcgacggt gtgccggatt
    13321 ttgtcgagtt ggaaaaaggg ctggatcctt ttggtggtgc ggatcatgac cgtgatggga
    13381 tctcggattt ggatgaactc atccgcggca cgggtgctgc ggatgcaaat gatccccgtg
    13441 gcagtacgaa agagaatccg ggcggcaatg cgccggtgct gcgcggcact ggcttgcgct
    13501 ttgtcgcgct gccctttgac gtgctgggca gccagatgaa ccctaaccag ccggtgcaat
    13561 tgcgtaccct cactggtggc ttgctggcag aggccaacac agcggttttc gatccccaac
    13621 tgcccgacaa cagtatcttt ggagcccggc tgagcaccag cgccagcgtg ccggttgagg
    13681 aactggtggc gctttcctcg cctgatagct tcaccacctt caatggtgcg gcaggtcgcg
    13741 agatcattcg ctttgtgcca gccgatccgc cgccgcaact gagtgtgtca atcacgccca
    13801 cgggcgcgaa cctgaccggg gatgcaaatg catggatcaa cgccgcgagg gctgccgtgg
    13861 ctgccgtgcc ctttgcggcg gcacgcacgc gcctggatcc acctgacatg gcggtctctg
    13921 ttttgctgga gcacctggtc cacagcgcgc tggtctcggt gcgttcggtg gagaatcccg
    13981 cgccctcgct ggaggacttc agtcttttca gccaacgccc cagtgatgga ggaagactga
    14041 ggctgaatga ggaggacaag gtgctgctca tcacgcaggg gtttctattc cgcaaggcgc
    14101 tgacccagtc gcagatccgc cgcaccgcca tgtccgcatt ggcaaacggc atttatgcac
    14161 ggcattccgc cgcgtcaaat ccgcccagcc tggacttgcc gctcgacatc ctgcgcgtcg
    14221 tgctgcgcgg cgaggacctg ccacctgagt atgcgggcgc ggccacttcg gggcaaatcg
    14281 ccacggcgcg tacggcttac aatggcgcgg tggcttctgc cctgggtaac ttccgtcctg
    14341 ataccattat cgaggtggaa atccccccca ctcctcttgg agagggtatc tatctcggcg
    14401 acttcggcgg cgaactggag ccggtggcgc tgttccggct cggacggcgg tttttgctgg
    14461 atgaccggtt cggctcgcag cccggcacgc gctttagcat cagggctttc accgatctgc
    14521 cgcctgaccg gggccgggtg gcgctggatg tgatttcggc ggagttcctc tccagcccgc
    14581 ccaacctgga ccgcgacagc gacctcaacg gcctggacga cgaatgggag cgctttttct
    14641 tcggttccat cgggcagaat ccaggtttca tccctccagg ccatacaaag accctggctc
    14701 aactctttgc caacggtgag gacccgcgcg cgctcgcagt ggagcctgac cttttggtgg
    14761 agcagccggc gggtaatgtg ctggccccat tggcgggggt gaacttcggc gtcatgggct
    14821 tgaactcctc gcagaacctg gttttcacgg tgcgcaacac cgggcgctcg gatttgacgg
    14881 gactgggcat cagtttcagt ggcgatcaag caggcagctt tagcgtgacg ctggagccgc
    14941 tgactccggt gacgccgcag gcccagtcca cattcaccgt gcgcttccgc ccctccaccg
    15001 ccgggtcgca ttcggcggtc atgtccatcg ccagcaatga cccggatgaa aatcccttcc
    15061 aagtccagct ttcgggaacg ggtgtcattg ttggtcatcc gcatccggga tttagcccgg
    15121 tcattggctc tgcaaatatc atcgccctcg ccacgcagcc ggacggcaaa atcctggcgg
    15181 gtggactcct gaccagcgtg aacgggcagg ccgtggggaa tctggcgcgg ttcaatgcgg
    15241 atggctcgct ggacaccagc ttcaacaccg gcacgggtgc cggcagcagc gtcatctgcc
    15301 tgacggtgca ggcggatggg aaaatcctcg tcggaggcaa cttcaccacg ttcaatggag
    15361 ccacggccac gcgcatcgtg cgcttgaact cgaacggcag cacggacagc agcttcagcg
    15421 ccgccttctc cggcggtgtc gggaccgtca ctggcattgt cacgcagccg gacggacgtc
    15481 tcattgtggt gggcaactac accagcgcca acggccaggc gcgcaaccgc atcgtccgct
    15541 tgctgccgga cggcacgctg gacggcagtt tcaatcccgg cacaggcgcg gacgacaaca
    15601 tccgcaacgc ggtggtgcag gcggatggga gaatcgtcat tgcgggtgac tttgcgaatt
    15661 tcaacggcca gccgcgcaac cggctggcgc gcttgaacgc ggacggcagc ctggaaagca
    15721 cggccggctt caatcccggc actggaccga atggccttgt gcgctgcctt tccctgctgc
    15781 cggacgggca gatcatgatt ggcggggtct ttaccacggt gaatggtttc ccgcgcagtg
    15841 gcctggcggt tctttcgtca tcgggaacag tcggcgcctt cacctgccag gggatcacgc
    15901 aggtcttttc cttcatccgg cagacggatg gcgatgtcat tgtcggtggc ggcttcacca
    15961 ccatcaacag ccaggcccgg aaccggatcg cgcgcattac accagcaacg cttggaagcg
    16021 ccgcattcgt gcccgacacc accttccagg tgggaattgg agcgaacaac acggtgaatg
    16081 ccgtggctct cgcggcgaac ggcggcgtca tcgccggggg attgttcacc acgatgaacg
    16141 gcctgccgcg cagcgggctg gccgtgctgg tcaatgagtc gccggccacg aacatcaccg
    16201 tcactgctga caaccgcatc ctctggtcgc gcagcggcac cgcgcccgag atcgcggagg
    16261 cggagttcga tgtctcggcc gacgctggcg ccaccttcac gccgctgggc acggcgcagt
    16321 tcatcgccgg aggctggcag atgaacgagg tggacttgcc tgatgagggc ctcattcgag
    16381 tgcgcgccgc cgtgcgcggc gggcagggga acagcagcgc aggacttgtg cagaccagcg
    16441 cgcccttcag tctcaacacc accagcacgc ccgtcatcag ttccatcgcc gcggtgacca
    16501 cggacgagga tgtttccgcc ggtcccatcg cgttcactgt ctcagacacc catacatctg
    16561 cggagcggct gcaggtgacg gcggtgtcca gcaacagcac cctgctgccc gcctccggca
    16621 tcgcgctcgg cggtgcggga gggcagcgca cgatcacgct gacaccggcc ttgaaccagt
    16681 ccggttcggc cacggtgacg gtgcgcgctt ttgacggcgc gcgcaccggc acgcgctcct
    16741 tcactttcac ggcgcagcca gtgaatgacg cgcctggaat ttcacccttt gctgatcatg
    16801 tcacggccct caacagcccg gtggtcatct cctttgccgt gtcggatgtc gagacgcctg
    16861 ctgagtcgct gatcgtcacg ggcacatcca gcaacacagc ggtggttccc ttggacggca
    16921 tcgttttgac gggcggcagc aaacgcgaag tcaggatcat ccacgcgcca aatgccgtcg
    16981 gcagcagcac catcaccctg accgtcagcg acggcgcggc tacggcgcaa agcagcttcc
    17041 aagtcacggt gaacaacagc gcgcctgtca ttgctaaaaa gtcagacatc accttgaacg
    17101 aggacttttt catcacagcg acccccatca ccatcagcga caacgagact ccggcggata
    17161 accttgtggt gacgttggag agttccaatc acgccctgct gccgccttcc cgccttgatg
    17221 tttttggcac cggcggctca cgctttttgt ccatccagcc tctgcctgac cagaatggca
    17281 gcgcggttgt tacggtgaat gtgagcgatg gcctgctgac cacggagatg agtttcaccg
    17341 tgtccgtcac acctttgaat gacgcgcccg tcatcgctga aatcgctgat ttgagcacgc
    17401 gggaggacac acccgtaacg atccccatca gtgtcaccga cattgacggt cctgtgattg
    17461 cgctcggtgc tttccaggtc aatccgccgc ccggcgcggt gcatggggtg acggtgaatc
    17521 aggccgacag aactgttctc atcacacctc cgcccgactt ccacggcacc gccaccttca
    17581 ccgtgacggt caatgactcg cgcctgagcg atgccaccac tttcaccctc accgtgctgc
    17641 ctgtgaatga cgcgccctcc tttaccaagg ggccggactt tgcgcatcca ttcggtttca
    17701 acacaccgca gacggtggaa aattgggcca ctgatctgag cgccggtccg gccaacgaag
    17761 cctcgcagaa tctcagtttc cgggtgcttt ccgtctcgca gcctgccgga ttgaccgaac
    17821 ctttctttgc cgccgcgccc accctttcgc cgcagggcag gctgaactac cgcactcgcg
    17881 gcctgggcgg aatctccacc atccggctgg tcatcgagga tgacggaggc acggagaatg
    17941 gcggcgtgga tcgcggggtg gagcaaacct tcaccatcac ggtcggtgac gcgcagccgg
    18001 tgctcgtgat cgaaacagag aacggccagg cgcttgagga aggagtgggc gttctgaatg
    18061 tgggcagcgt cacgccaggc ggatctgtcc ggcggcagct cacagtgcga aatgcgggtc
    18121 aggctcccct caatttcggc ggagtcgtca aaggcggggg actggagcgt ttcatcgtcg
    18181 gctttgttca aacgggtgtc ttgctgccgg gtgaatcgcg ccaggtgcag attgcttttg
    18241 cgccggatac tccgggtgtg cattcaggag tgctgacctt cctgagcaat gatcccgtcg
    18301 aggccaggca gaattactcc atcaccctga gtggcagtgg cgcctccgcc ccggtggtgc
    18361 ggctgctgcg gccgaactcg gtgacgcgcc agagcgtgga tctcagcgcg gttgtcaatg
    18421 cggggaatgc cgacaccacg gtgacggtgg acctgggcac gacgattgac ttcggcatca
    18481 ccgtgcccgc ccagccttcc atcgtgagcg gcggggacga aacgccggtg cgcgcggtgg
    18541 cctccggtct gctaggcggc acgttgtatt tttaccgcgt ccgcgcccaa aacgcggcgg
    18601 gtgtgagcac cgctcaaggg gtgtttttca ctgagaccaa caccgccggc atgttggacg
    18661 agagctttct cccgcaggtg gatgccaatg gccgcgtgct tgcgctggca gtgcagatgg
    18721 atggaaaaat ctgggtcggc ggcgacttca ttgagatcaa tggcactccc tgcgcggcgc
    18781 tggcgaggct gaatccggac ggcagcctgg acaaaatctt caatctgcga aacgaaggca
    18841 gcgaggttct gcccgaggtg cgcgccatcg tggtgcggcc ggatggcagg gtggtggtcg
    18901 gcggcgtctt caaccgcatc aatgacacgg catgccattt gctcgtccgc ttgaattccg
    18961 atggcacgtt ggatgatgat ttcgcgaatg tgaccttcgg caccggcccg gccgacagcg
    19021 tgctgcggct gcttttgcag acggatggct ccctgatcgt gggcggtcgt ttcagcagcg
    19081 tgaatggcgc acctcgcact tccctggcgc gcctgaggga tgacggcgcg ctcgacacaa
    19141 ccttcgcgcc cgttcttggt ggaggaggtc cgctgacgcc agaggtgctc agcatggcgt
    19201 cacaggatga tggacgcatt ctcatcggcg gacttttcgc caccgtaaat ggagcgccgc
    19261 gcgtgaatgt ggccaggctt ggcgaggacg gcacgctgga tgccggcttc gacgcgcgca
    19321 tggagttcca ccaggggctt ggcgaggtga acagcatcct ggtgaaaccc gatggcggca
    19381 tcctgctcgg cggcgcgcag ctccggttcc ttgaccgctc accgggcatc aacgacttcc
    19441 tcgccgctct gcgtgaggat ggctcggcgg gggaaggggt tgccctcgac atcgaacagg
    19501 cgctgagttc atcctctcag ggtgccgggc cgctgccctc caggttgaac gtgcccaccc
    19561 tcaccgactt ggccgcgcag gccgatggca gcgtcgtcat ggccggaaat ttcacgctca
    19621 taagcaatgg acggctggtg aacaacatcg ctcgcatcct tcccgatggc acgctcgacg
    19681 cggactttca ggcggacgtg ccggtgcgcg cgctcgaatg cctggcgatc cagggtgacg
    19741 ggcgcgtgct ggccggaggc tttttcaccc aggtgaatgg cgtggcgcag ttcaatcttg
    19801 cgcgcctgct caatgacgcg ccttttgagt ccctggaggt ggtggatggc agccgtgttg
    19861 agtggcggcg cggtggagcc gcgcccgagg cgcgggagct tctcctcgac atttccacgg
    19921 atggcggctt gagcttcgcg ccgctcggcg aagctgtgcg catccccggt ggctgggcgc
    19981 ttgtggatgc gggtctcgct gagctggatg tcttccacat ccgcgcccgc acccatctca
    20041 tcagcggtgg tggcaacggt tccgctggca tcatgcaaag cgtcatcgcg cacaaccagc
    20101 ctgacatcga ggtcctgacc ctcgacaaac aagaattcgt ggatgacgtc agcacgcatg
    20161 acttcggccc ctcgctgctg accgggttgg tcacgcaaac cttcgtcatt cgcaatgccg
    20221 gaccgctgcc cttgcagatc ggcgccatcg ctgtggatgg cttggcggcg gcggattacg
    20281 cggtcaacac caccggcgtg agcgcgattt tggagaatgg ccagatcacc accttcacca
    20341 tcaccttcgc gcccaaagac accggcttcc gcgaggcggt gctgcgcatc gccagcaacg
    20401 atcccgatgc ggaatcctct ttcgatgtgg atcttctcgg cgagggcgtg ccgccgggac
    20461 cgccgcagat ggtggtcaga ggaccaaacg gcgaagcgtt tggtcatcgc gcgaaactcg
    20521 aattgggcag cgtcttgaaa ggccagacgg cgggctttgt cttcaccatc gagaaccagg
    20581 gtttcgcccc gctcacgggt ctggctgtca gcaaaatcaa cggtggcaac ggcttcacct
    20641 tcactccggt ggggagcgcc tcgatctcgc gggggcaaag caccaccttc ctcgtcagct
    20701 tcaagccacc gaattcaaca ccgcagggca tgcggctgag cctcgccagc aacgatccgg
    20761 cgcagactcc ttttgaattt gacctcagag ccgcggaggc tgtgcgctcg gtggcggtgg
    20821 acttcaatgg gacgcggcta aaatccggtg ccgcctcgct gcaaaacctg ggcagcaatc
    20881 tgacgttcgg cgtgccagtg cagcgcacct tcaacctggt gaacacgggc aatgtggctt
    20941 tcgtcatcgc caatctcgtc gtcaatcatc cggattatca ggtgtccgct cccggcaaag
    21001 tgcagcttgc ccccggcggc gattccacgc cattcaccgt cacctttacc ccgcgctccg
    21061 gcgggctcag ccatgcctcg gtgcaggtgc tgggcaatga aggaccgcag ccgttctttg
    21121 atctcaggct gcaaggtggg atacaggccc aggagattgc cgtggagcag ccggcgggcg
    21181 cgagcttggc cagcggatcc tccgttgtgg atttcggcac gcctctggcg ggtggccgag
    21241 tggttcgtgt cttcaccctg cgcaacacag gcaccaacac gctcaacctc ggcgctcccg
    21301 tttttgaggg gggagcgcca ttcacactca ccaagctgcc gcccggcgcg cttgtggcag
    21361 gcaaatccgt caccttcgag gtgactttcg cgccggccgc aggaggcgac tttgccggca
    21421 acctgctgct gccaaacaac gatctggatg aaaacccgtt caggatcgaa cttcgtggtt
    21481 ccgccctgcc atcgctgctg ccggtcatca caggccatcc gcagtcgcag attgtgcccc
    21541 tcggagcggc tgtgagtttc gatggcggag cggcatccga gacggccttg agcttccaat
    21601 ggcagcgggg tctgggagcg ctgtccaact tcggcaaagc actggcaact tccccgctca
    21661 ggatcgccaa agtcacgctc aatgacgcct ccaactaccg cttccaggcc atcaatgcct
    21721 ttggctcgac ggccagcgat tccgcacgtc tggtggtggt ggacaccaaa gtctccgctt
    21781 tcaatctcac gcccggacgc gcactcgccc tgcgtttgaa tgtggccggt ccggggccgc
    21841 tggagctatt gtggcacaag gacggagcac ctctgacggc tagccagcgc gtcactggag
    21901 tcacaggcca ggatctcgtc atcaacaacc tcacgactgc tgacagcggc atttacacct
    21961 gtgtcgtcac cacaccccag ggcgtgctca caggcgggga aaaccgggtc aacgtggtgg
    22021 aactggctcc tcaaatcacc ctgaatcccg ccgtgatgcc cgacggacgg atcggccttc
    22081 catactcctt cttcatcccg ctgaatccgg agcgcggtct cgccaccacc ttcaacgccg
    22141 ctggcctgcc ggcaggtctg gtcttgaatg cggccaccgg tttgatcagc ggcaatccca
    22201 ctcagagccg tacacagcct ttcagtgtca ccctgaaggc cacgaacagc aaaggctcgt
    22261 ccaccttggt tgcgacgatc agcgtggctc cgctgccgga ggggctggcc ggggtatatg
    22321 ccggcatcat tgagcgcgcg gcgatgaacc gtcatcttgg cggccgcttt gatcttgccg
    22381 tggccggcac cggcagtttc actggcaagg tgctgctggg ggccggcatc caggacaatc
    22441 tgcagggcgt gctgcgcctg tcacccggca gcccgctggt cagcgtggag atcctgacag
    22501 gcgggcgcgc atacactttc acgctcaatc cgcagagccg gcgcatcacg gacggggtgg
    22561 ccgcttccgg ggagggaacc gcggacttca tcggctggag ggaggcattc aatgaaagca
    22621 gcggcgccgg gctggccttt gcaggcagtc acaactttgg catggagatg ccctcaaccg
    22681 tgcgcgggaa acccggcttt cctgaaggca ccggcttcgg ccattgcaat gtgcgcaatg
    22741 gcggacgcga cgctgtgatc accggcaaga cagccgatgg tgaagcctgc accggcggca
    22801 gcgtgttgag tgataatggt gaggtggtgg tcttcagccg cggcaccaag ggttcgctga
    22861 gcggagttat gaaggtcctg cctgacctcg cgcccccgtt tggcaacatt cgtttgggcg
    22921 gtgagttgag ctggatgcgc ctgcctctgg ccgggcgtgt ctatccggca ggattcggac
    22981 cgttgaacct ccgcatcttt ggcggcaggt accgggagcc ggcattcgcc accgtgttgg
    23041 gatcacccac cgcgcgcctc accttcgctg gcgcggacat cggtggtcct ccatcattgg
    23101 tggatcggtt gttcaccctg ccttttggag tccaatcctt cacgctgaag gacagggatg
    23161 tgactctcaa cgtgcgcccc ggcgccaacg cgcgtctcga aagcggcggc tttagcggca
    23221 gtttcaaatt cacccagcgc gacgtggcca acccgccctt caatgtcctg acacgcagcg
    23281 ccagctttga aggcgtcatc atcccggacg gggaattcca gcgcggagtc ggctttttca
    23341 acctgccctc catgccccag cgcggccaga cagccaccca gacgcccatc ctttcaggca
    23401 gcgtccagct cgacccagtg cgttagcgcg gttcaatggc ggacggtgtc ctgcctcgct
    23461 cgtttgagtg gtgtcttgcg cgcatttgcc gcggcttgct gtatccgaca cagtttttgc
    23521 tggcagtttt ctggggagca aggcgtttat ctgagcacaa ccatgaagtg cctgattctt
    23581 tccttcctct tgctcgcccc tctggcagtg acctgcccta ttttggccca agtgcctaaa
    23641 gcaagtgcga agaaaaaggt caaccggcct caggcggtcg gaccggccat cggcgcgaat
    23701 gtggcgacgc cggtgtcgaa catcaagacg ctaaaggact tcaaggtgga gctcatttac
    23761 agcgtgcctg gcggcgagca gggctcgtgg gtgaacctca cgacggatga cgcgggccgc
    23821 atctatgtga gcgaccagta tgggatgctc taccgcttca aagcgcccgc cgcaggccag
    23881 gcgctgagcc agggcgacat cgaaaaagtg cctgcggaca tccgcggcgt gaacggcatg
    23941 ctctttgcct tcgatgcgct gtatgtcggc gtgaatgatt atgagaaaaa aatccccagc
    24001 ggcgtgtatc gcatcaccga cagcaacagc gacgacatgc cggacaaagt ggagatgctg
    24061 cgcgagttcg acgcgggcag cgaccacggc gtgcatgcca ttttgaagac gccggacggc
    24121 aagggccttt acctcatcag cggcaacaac gccgagatca aggagggtcc gaaggccggc
    24181 acgcccgaca gctcgcccgt cgcgaagttg tggggcgatg accacctgct gccgcgcatg
    24241 ccggacggtc gcggtcacaa ccgccacgtc atggcgcccg gcggcatcgt gtatcgcttc
    24301 acgccggatg gaaagacctt cgaaatcttc gccagcggct tccgcaacat ctacgacggc
    24361 ggcgtgaacc gcgacggcga gctcttcgtc tatgacgcgg acatggagta tgacttcaac
    24421 acgtcgtggt atcgccccac gcgtatcaac cacgtcgtca gcggcgcgga gttcggctgg
    24481 cgcaacggcg cgggcaagta tcccgagttc tacatcgaca accttcctgc cacgctgaac
    24541 atcggccccg gctcgcccac cggctgcacc ttcggctacg gcgcgaagtt ccccgcgaaa
    24601 taccaggagg ccttttacgc gctcgattgg agctggggaa aaatctacgc cgtgcatctg
    24661 acgcccaacg gcagcaccta caccgccacg aaggaagaat tcgtcaccgg cggcccgctg
    24721 cccgtcagcg atgccatcat cggccaagac ggcgcgatgt atttcaccat cggcggccgc
    24781 cgcgtgcaga gcgggctgta ccgggtgagt tatgtgggga aagagagcac tgcgccgtct
    24841 gacaagtccg accggtccga cgagtcagac aaagcggtgg cgctccgccg ctcactcgaa
    24901 gcattccacg gcaaacaaga cccaaaagcc gtcgccaccg cctggccgca tctcagcagc
    24961 acggatcgct acatccgcgc cgccacccgc actgcgctgg agcatcagcc gctcgccgaa
    25021 tggcaggaca aagcgctcgc tgaaaccaac gtcaccgcgc agctcgaagc cttgctcgct
    25081 ctcacgcgcc gcaccggtgt gtgcccgact catcggaccg atccgtccta tcagcccgat
    25141 tccaaatccc gcgacgccat cctcaccgcg ctgctgaagc acgacttcgc gaagctcacg
    25201 aacgagcaaa agctcggcta tatgcgcatc atcgagatcg tcttgcaccg ctttggcaat
    25261 cccgacgacg ccaccgtcgc cgcgatcatc gcgaaactcg accccgcgta tcccgccgac
    25321 aacttcgagc tgaactggct gctcaccgag acgctggccc acctgcaagc gccgaacacg
    25381 gcggcgaaag ccatggcgct catcgccgag gccggcagcc aggagccgga gatcgaatat
    25441 gcgcgcagcc tgcgcttcct gaaagctggc tggacgccgg aactgcgcac gcagcagctc
    25501 gaatggtttc tgaaagcggc cagttacaag ggaggttcca gctttaccaa gttcatcgag
    25561 ttcatccgca acgactccct ggccaccttc accgaagcgg agaaagccac gcacgccgcg
    25621 ctcatcgaga agaagccgga gatcaagagc gccatcgaaa atgtcggtgc catctttgtg
    25681 ggccgcacgc ccacgatgtg gacgctggag gagctgagcg ccgccgcgca gaccggcatg
    25741 aaggggcgtg actttgacac cggacgcaag atgttctccg ccgccgcttg ctacacctgc
    25801 caccggtttg gcaatgccgg cggcatgacc ggccccgacc tcaccggctc cggtggccgc
    25861 tacagcccgc acgatctgct ggatcagatc atcaacccga gcaaggagat caatgagcag
    25921 ttcgccccga tcatcgtcac gaaaaacagc ggcgagattt tgagcggcgt ggtggtcaat
    25981 ctcagcggcg acaacgtgac gctgaacacc gacctgagcg accccgacca gcgcgtgaac
    26041 attgaccgca aggaggtgaa aagcatcgag ctgagcaagg tctcgcccat gccgccgatg
    26101 ctgctggcca tgctgaagaa ggacgagatc ctcgacctcc tggcctacat cctcagcggc
    26161 ggtgacaaag gccacgccat gtttgtgaag tgatcacagc gcccgcattt gcaggatttt
    26221 tcgttttccc cgccgccgcc atgcgctaga aaaggcgcgc atggttcaca tctccatccg
    26281 cgaagtcagc aaacgttttg gccgccagac cgtgctggac cgcgtcagcc tggagatcga
    26341 ggcgggggag ttgttcttcc tgcttggacc cagcggttgc ggcaagacca cgctgctgcg
    26401 gcatgtggcc ggtttttacc agccggacga gggccgcata ttctttgacg aggatgatgt
    26461 gacacgcctg cccgtccaca agcgcgacac gggcatgatg ttccaaagct acgcgctctg
    26521 gccgcatctg aacgtggcgc aaaacgtcgc cttcgggctg gaggaaagaa ggcgccctca
    26581 agcggagatc gagcaccggg tcaacgaggc gctggagctg gtgcagctcg gcgggctggg
    26641 agtgcgccgc attgcgcagc tctcaggcgg gcagcagcag cgcgtggccc tggcgcgcgc
    26701 gctggtggtc cacccgcgct gcctgctgct ggacgagccg ctctccaatc tcgacgccaa
    26761 gctgcgccac gagatgcgcg cggagatacg ccgcatctgc aagcagttcg gactcacggg
    26821 catctatgtg acgcatgacc gcgacgaggc gctgagcatg gccgaccgtc ttgcggtgat
    26881 ggattcgggg cggctggcgc aggttggcac gccggaggag gtctatcgga atcccgcctc
    26941 gcgcatggtg gcgggcttca tcggcggcac gaacttcatc gaggccgtgg tggccgcccc
    27001 tgcgggagcg gacggcatgc atgaggtcca caccgttttc ggcgcgctgc gcgccaggtc
    27061 taactccgcc tcatggcggc ccggcgcagg agtcaaagtg ctgctttccg tccggcccga
    27121 ggcgctggct tttgccgcgg caggcgcggg tgaaaacagc atccccgcgc gcatcaccgg
    27181 cgccacttat ctgggtgcca cggtgcagta cgacatgcgc gtggatggcg gccctgacct
    27241 ccatgcatgc gagaccaatc cgcgacttgt gcgccagcct tcagaagcgc ggatctctct
    27301 caccgtcgcc gcagaggatg tggtgatgct gccggtggaa acgcggcatt gagcccatga
    27361 cgagcatgtc cccggtcatc gccaccctcg ccgaactcgt gcgcatcaac agcgtcaact
    27421 cctcctatga gggcgggccg ggggaagggg agatcgcggc ccatgtccgc cgctttttcg
    27481 agaagcacgg catcgaggtc cgggagcagg aggtctttcc cggcaggccc aatgtcatcg
    27541 cccggctgcc ggggagaaat cccgcgcgcc gcgtggtgct ggaagcgcac accgacactg
    27601 tgtccgtcca aggcatgacc atcccgcctt ttgaacccgt catccgcgat ggcaaaatgt
    27661 acgggcgcgg ctcctgcgac acgaaggccg gcctcgccgc catgatgcac gcgctggctt
    27721 cattgaagga ggagggcgtc accccgccgt gcgaggtgct gctcgccgcc gccgtggacg
    27781 aggagtattc ctaccgcgga gttgtgcgtt tttgcgaggg gctgcgcgcg gacgcggcca
    27841 ttgtcgccga gccgaccgag ctgcgcgccg tcatcgccac caagggcgtg ctgcgctgcc
    27901 gcatcgtcgc gcatggcaaa gccgcgcaca gctccaagcc gcatctcggc gtcaatgcca
    27961 tcaccgccat ggcccgtgtc atcggcgccc tggagacgga tgccggtcgt ttcgcgcaaa
    28021 accgccatcc tctcaccgga caagccacgt tgaatgtcgg cgtcatccat ggcggtgtgc
    28081 aggtgaactt cgtccccgac cgctgcgcca tcgagattga ccgccgcctg ctgcccggag
    28141 aacaagcaga gaccgcgctg cgcgagtatg aggaactggt ggcaaaagtc tgcgccgccc
    28201 tgcccggcgt gcgcgcggag atcgagcagc cgcctctgct cgtggatgag gcgctcgaca
    28261 caccggcgga ctccgccgtg gtccgcacag cccgcgccgt gctggatgag atgggccttg
    28321 actccgcccc gtgcggcgtg cccttcggct gcgatgccag caagctctcc cgcgccggca
    28381 tccccgccat cgtcttcggt cccggcagca tagaccgcgc ccacaccgcc gatgaattca
    28441 tcgagctgga tcaggtggag cgcgccctgg agttcca
//
LOCUS       DAOMYJ010000043        28291 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Prosthecobacter sp. isolate AMR_MDS_5629
            NODE_597_length_28291_cov_21.986294, whole genome shotgun sequence.
ACCESSION   DAOMYJ010000043 DAOMYJ010000000
VERSION     DAOMYJ010000043.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867850
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prosthecobacter sp. (wastewater metagenome)
  ORGANISM  Prosthecobacter sp.
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Verrucomicrobiaceae; Prosthecobacter.
REFERENCE   1  (bases 1 to 28291)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 28291)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.30
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:34:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,742
            CDSs (total)                      :: 4,684
            Genes (coding)                    :: 4,676
            CDSs (with protein)               :: 4,676
            Genes (RNA)                       :: 58
            tRNAs                             :: 55
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28291
                     /organism="Prosthecobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_597_length_28291_cov_21.986294"
                     /isolate="AMR_MDS_5629"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1965333"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(33..251)
                     /locus_tag="PLP58_06650"
     CDS             complement(33..251)
                     /locus_tag="PLP58_06650"
                     /inference="COORDINATES: protein motif:HMM:NF016430.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor"
                     /protein_id="HRJ08230.1"
                     /translation="MAPAADIWKRCFEQVAPRLLLYACQLCPSRADAEDVVQMAFVRW
                     WRRFPDGDENHIPLLYAAVRTMIGSSER"
     gene            complement(322..1260)
                     /locus_tag="PLP58_06655"
     CDS             complement(322..1260)
                     /locus_tag="PLP58_06655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009959921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histone deacetylase"
                     /protein_id="HRJ08231.1"
                     /translation="MSTGLLQDPVFARHDTGSGHPECAERHAAVTRALAEAGLTARLR
                     PIVSRPAEPPELERCHTRQYIELVREEIAAGLDTLSTGDTQISPGSHDAALHAVGGVI
                     EAVDSVMTGKLSRAFCAVRPPGHHARPAQGMGFCVFNNIALGARHAQARHGAGKVVIV
                     DWDVHHGNGTQDIFYEDGSVLFFSTHQSPWYPFTGHAEETGAGKGRGSTLNCPLAAGA
                     GMKEIGAAFEEKLLPALESFKPDLIMISAGFDSRERDPLGRFRLTDTDFAALTRLLKQ
                     AADEHCQGRLVSVLEGGYNLEGLAKAVTAHVVELAV"
     gene            complement(1329..3146)
                     /locus_tag="PLP58_06660"
     CDS             complement(1329..3146)
                     /locus_tag="PLP58_06660"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HRJ08232.1"
                     /translation="MPSQDSKLLHGLDPAALFSLADEAAPAAAGGAWEPPGMEEVAAL
                     FPQWQAKGLLGRGGMGAVYHFHQSDLARDVAVKLLPIEASADERQVERFRREARALAR
                     LRHPNIVALHEAGITPAGHFFFVMEYVDGQPLSRLIAGGKMDAPRAIEVVRQVCDALA
                     CAHGQGVIHRDIKPSNILINSAGQVKVADFGLARLDHAAEEDALTLSRTGAFMGTPAY
                     TAPEQARDAAHADHRADIYSLGVLLYEMLTGELPRGVFQPPSRKAGSDARLDSVVRRA
                     LQERPEDRYQAAAELKEDVTESASRPAADTSKAPAPWRAWAALLIVCVVVAAAALVFF
                     KQAPESKPDQHAPMTAREPQPKLPEPASEPPKPEAAQSYPAKKESPPMPAPVSSPETR
                     QEPPSPGALPPQFSKVHVWSLDPVPPALYPPASTTGAGWKDAVLIADGGAALLHDGSI
                     IIWSDREPGKGLPIVWPPRSTAGLILPGDRKSVLAEPIVRLTATATDAVAWAESGAAF
                     LLPREPGAGLRRLLPAEAEKFPAHMDGRPQSDIQGSKPAGLSSQARVIQQEGIWLGWE
                     SDGKATLWGPRISDSPQRFRLPEGVTQVRLGPTGLMAAW"
     gene            complement(3178..4194)
                     /locus_tag="PLP58_06665"
     CDS             complement(3178..4194)
                     /locus_tag="PLP58_06665"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HRJ08233.1"
                     /translation="MKLHHLLLLLATTGAFAQQKLVPTKDAPNTKDYPGMPRYEGSTI
                     VLQSEQKFGELGLQIGGLSSPVSTDPKEVRKVEGRTHRSTYLYLNLGAGKRSTLEIAR
                     NYEIAFKDAGFAEIWSGGDREIRNGPPRAYYGVPELDHQLMTTGLKERRYFCMEKSGL
                     FAAVFIAARSWDHPMLPSSEANPWKQHITIPQDSVVIQVDVVNTRPMEEKMVHLSAAE
                     MRKSISSSGKVAIYGILFDFNKADIKPESAPTLAEMAALLKAEPNLKVLVVGHTDNVG
                     TFEFNEDLSKRRARAVVAELAAKHGIAADRMLPLGASFMAPVTTNSTEEGRALNRRVE
                     LVAR"
     gene            complement(4254..4616)
                     /locus_tag="PLP58_06670"
     CDS             complement(4254..4616)
                     /locus_tag="PLP58_06670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ08234.1"
                     /translation="MIGILIGLLVPVLIVGAIIVLLVKRGLQFRELCEHGIETSGQVI
                     GKRSVKAGSSTSRRQKLVYRYTDSAGVAHEHTSVVTWDTYGRHDEGGPIEVVYSSKNP
                     AVSAPKYLVDQAKSALGK"
     gene            4713..5447
                     /locus_tag="PLP58_06675"
     CDS             4713..5447
                     /locus_tag="PLP58_06675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ08235.1"
                     /translation="MTTASNAAFPVTRWSLVAGARAAADPNRKRAALEELCGLYWRPI
                     YGFLRRRGLSVAEAQDDAQGFFMSLLEEDLFAKAQAETGRMRSFLLAALQRWQRGEWR
                     KAQAEKRGGGNELFSLEVMAEEDGFEAAGNDDDTPERRFEKSCALALLEAAMQRLAKE
                     QSAAGKGENFDTLRPLLAPLGGETSITQEEAAKRLRMTAEALRVTLHRLRKRFAEVLR
                     ETVADTLTDPTEQAIQEEMDALRAAF"
     gene            5555..6904
                     /locus_tag="PLP58_06680"
     CDS             5555..6904
                     /locus_tag="PLP58_06680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ08236.1"
                     /translation="MKTKLTLLLALAVSASANAEKPNFNGFKPAQSASAPRAAPSYSG
                     GGSSRSFNPGQAIGIARSLLPLVMNATPPPPPRPSVVKRSPSPAVTTNKPATRSVSKP
                     ATVTRNPASGSRGALPAPVGGMDPKLAEGLEAGRQIASGLQSLESLRASFGSVEGLGD
                     VFHQPGNAGPGSQGGVRDPFGGGSEYDGGKVSNPLDRFSTAKPRDIRGGFSNSRRGNA
                     TTGLSRTGDRVLESEVSTASGRDGTSTPVQSTTHENGDVTASTTATTRDGNSTEVEVT
                     EFSDGSGTSTTTHSNDGNADTLIVTRRDSSGAVLWTRTDSVHAGGKQHIVTRDGSGRV
                     TSDTTEYYTPGSRTCGRSPGEIAAGGPVNGTGPGVADAAGLMPLDLLRQFANGEGASG
                     GTNTMTSGSLSSRHVNPDRNGSSGPMRNRLTVDTFGTISNPAPLGGEGAAGGGNRPD"
     gene            complement(6978..7982)
                     /locus_tag="PLP58_06685"
     CDS             complement(6978..7982)
                     /locus_tag="PLP58_06685"
                     /inference="COORDINATES: protein motif:HMM:NF020039.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMP-30/gluconolactonase/LRE family protein"
                     /protein_id="HRJ08237.1"
                     /translation="MKYLPLLLPLALSSCTVLTSPLVPGAKTRDHGSIGAVEGPAWKD
                     GSLYFTDGKHINRMGPDGRVSVFRHNASNGLLFDREGRLVACETKKRRVTRTEKDGSI
                     TVLADRFLGKRFNSPNDLCMDSKGLIYFTDPRYGSRAGMEIFDQVDGCEPSGIKPGQM
                     PIEGVYRINRQARLPDGLCAFAYDIPNVTRILCEGAERPNGILITPDDRFLYIADNNN
                     NTRGGSRKLLRYELDEYGDVKPGTRRVIFDWKDGRGPDGMKMDAKGRIYVAAGTNKPT
                     EYETTRYKAGCYILSGSGRLLDFVPTAPDECCNCAFGGADGKTLFITSGGNLWSAPLR
                     "
     gene            complement(8058..8825)
                     /locus_tag="PLP58_06690"
     CDS             complement(8058..8825)
                     /locus_tag="PLP58_06690"
                     /inference="COORDINATES: protein motif:HMM:NF012311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HRJ08238.1"
                     /translation="MSSYFHDEEALARTAIALGASSGAGVRVAILDSGVDTAHPALEG
                     LSLADDVMFEAGGNFLESKAGNGDAIGHGTAIAWIIRRLAPCAEIGSFRVLDGALRSR
                     STVIWEAARLALERGYQILNCSFGSPGEARFVMPYKEWTDAAYLSRAHIVAACSNDDA
                     GLREWPGWFPTVLTVNLAALDHDGWEHRTGAGMVEFAARGFEVTVPWQGGWKKVTGSS
                     FAAPVVSAWLARLVSLQPDLSVEQAKELMRRLAGRRM"
     gene            complement(8839..9486)
                     /locus_tag="PLP58_06695"
     CDS             complement(8839..9486)
                     /locus_tag="PLP58_06695"
                     /inference="COORDINATES: protein motif:HMM:NF013736.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HRJ08239.1"
                     /translation="MPAPFPALPDPDLADFEERFSRRRMDFLRRAQETRAAALAEGLP
                     ARGLDAALKNIEAHEGSLWLAVEDGAVLVPVWNNGPDAARFTGSFRLPAAEGITGMVF
                     TSGLAMCESEVCFHQRQNRALDRALGVLTWAMLAVPLRFAGEVRGVVTAVRLIRLDAL
                     PGLREAPRSRADLPPGHALPESFSLAHLAAMETAAQVLGRLLDHRLSAWALGWED"
     gene            complement(9605..14056)
                     /locus_tag="PLP58_06700"
     CDS             complement(9605..14056)
                     /locus_tag="PLP58_06700"
                     /inference="COORDINATES: protein motif:HMM:NF040218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BACON domain-containing protein"
                     /protein_id="HRJ08240.1"
                     /translation="MKTLLRLLQNSAALSLLLAAALPATAFDAATLKPLGVNVGGVNY
                     FTTPYFANAVAQGGEWLEFDTNFGDRVPHFNSAQFNEKGYPKFLNPGKKLRLLLFGLH
                     TDYGDLRHPSWPVRDSVLANGKIVVEWTGDADIRLNDGVFIPGESNGPETGSLVNGRR
                     VYLKSNASDNSFMQVESINPANPITDLKVWLPDPADPQNATLEDELFHPVFLQRLAEV
                     DWSFLRMMDWGVTNANPQKSWSDRRLPDHVFQCGVLNDRDPTDEFALSIGDRETGVAY
                     EHIIALCNAANKDLWICIPHLADDDFITKLAQMIRFGSDGVNPHTAPVADPAFAPLNP
                     NLRVYVEYSNEIWSNGFSFPQGNWAEAKAAQQGLTRPQFNARQFCRVWRVFEQVFEGN
                     DRLIKTAAVFTGSQSYIEEFLTEIKDHGATLTPPQEPDCISPTTYFGNDIQGFAFQRA
                     LEQRATIDPWFLTTGNFNFDGGQRPVSVTPESGYWVSDAINRHMDELFQEWRRRMLSG
                     STQTGSGPDATGIGGGFGDGLRALAQTLFTAPKPIVSYEGGPSLFTDELDGPDERDDG
                     ITAFMELVNRQPEMADVYEIHLNQALEKGLRSHGVFVESGGWGKFGQWGHLESIRQST
                     QDSPKWQFLLDWETELAGLRHIDDVLGSAPAFATAAKLPTAIHGTPYTTTISTTGGDG
                     ARALKIIGAVLPQGLNITLNGGEATLSGTPASTGESFLYLRVRDADGDPAWRTFFFRT
                     VGGPGVLAESNFEGSDPALNLPWTPTYVLKNGVTYSGWTAGAGITPEAGDDGLAYSQV
                     MPNNESTLAQAITAGQFWAVSVQGGSPLNLRNAEVRFTIRRLSFHAPRLHAVLTSVGG
                     FSAAQAVFTTTRLDGDTNEQEFAFRLPDTAAFENRGGPVEFRIYGFAGQFSGHMAMIR
                     SFKITPADAGAAQVLDPVENSIAAAGQSYTIAVTTPGAWTAKSSASFAVVNPKSGTGN
                     GSVTVNVQPNNSKKARTAKITIAGAVHALTQAAALPPTIDFPAAVAPLAVSADFSMTI
                     PTVNPPVKYTIKGKLPPGLVFNQLTGTLSGKPATPGEFDFSITAANAAGAAPQKLDFS
                     LQVTDLDGRHIGVFHGLAGRLSNLNNGLGSRVELTVAKTGVVSGKILTGTVSHAFTGR
                     LDTFTEIVKKPKLVTDIKRGKLSTLVLDVEFDPDTNSLSGTLNEKDSPGISTQVSAWR
                     NGWTKDEKATAFKAAYTFAIEPAAANTGIEAIPQGDGYGSFTVPDNGVVKVAGKLADG
                     SAYTCSTFVGQQGEVLLYAGLYKNRGSLAGALEISGGFIGALQDLEPDWFKDEPLPNS
                     KERNYKAGFGPDRVEVNGGIHAPPAPGGLLMGLDAGQNNAALLFSAAGLTHSEGEAPD
                     VLVTLANPSATGIVNNAGVPKAGTAENEGKVSIKLVSKTGLFSGRLTVPSAIGAAPRT
                     VSYTGMIVNRPGASAGHGYFLLAKEPAPGQKANETDILSGLARLIGN"
     gene            14239..15858
                     /locus_tag="PLP58_06705"
     CDS             14239..15858
                     /locus_tag="PLP58_06705"
                     /inference="COORDINATES: protein motif:HMM:NF024627.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HRJ08241.1"
                     /translation="MKLLPALFCLAPPVCADDSARLAELLARGGVVTIPPGDYQLNGA
                     QPILLVSNTTVLAHGARFILPETLPDQARLIIFAGQDIGHFTWQGGEFIGHVFDPARR
                     ENAWPPNANTKGIEITTTRDGGTHGIHFRDVKSNGMAGAVIGVHGLVKKGGENEVLAH
                     AERVAVESCTLLRSGKFMWDYGYLWQIMTWPEEYESWEVERARQYFRTDLIREVEFIS
                     GGDRVLFDNTNRPLAVSQTDSPQEAITFMGADLPPGIERGRQYFVTESTPEFIKIANQ
                     PGGQAVRFGGDGKGSLVFFIHAAYLSAYAPTGSGPGKGAFDITGARDVRVTGCQLSAL
                     GDTMHIQACHNIVFSSNHILGSRMGAFFLAEFCQNATITGNLVDGTNGSRVMSVEKSC
                     TDVTITGNTFRNGGRGSWINQPVNFIMTGNVFVNNTTKNETDMRRGRIAYQTGLPVRF
                     PELYFTLHEPDAAYGPVIVRDNIFRLGDSAPEEAVTFAPNGKDVQFTGNIFQNKPAII
                     VVDPSCSGTRVEDNPGATTVSRPVDFNHGRR"
     gene            complement(15890..16588)
                     /locus_tag="PLP58_06710"
     CDS             complement(15890..16588)
                     /locus_tag="PLP58_06710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009961380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-carboxy-7-deazaguanine synthase QueE"
                     /protein_id="HRJ08242.1"
                     /translation="MRISEIFYSIQGEGALTGVPSVFVRASGCNLRCAWCDTPYASWS
                     PEGGEMSVDEILDRVAQHPARFVVVTGGEPMVARDIRVLLARLREAGRHVTLETAGTV
                     PPEGARVNLASISPKLANSRPDPASTGTAWVERHEHTRLRPEVVRQWMESADDYQLKF
                     VISNEADLREMQGVISAIGAPVPPEKILLMPEGTDMEAMRRRYDLLVNACKDHGWRFC
                     PRLHIELFGNKRGT"
     gene            16663..17169
                     /locus_tag="PLP58_06715"
     CDS             16663..17169
                     /locus_tag="PLP58_06715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009961379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan endopeptidase"
                     /protein_id="HRJ08243.1"
                     /translation="MPHFRRLLLFTAFLCASCASKPKMWHYQYEKGRTAILVNGQAVP
                     PAGLPKKVMQAVSAGNRIAGLPYKFGGGHRSFEDNGYDCSGAVSYVLHAAGALKSPGT
                     SESLRSFGKKGAGKHITLYTKPGHHCFLVVAGLRFDTGYNGQRKGPEWSTKSRPVTGY
                     TARHPPGL"
     gene            complement(17185..18075)
                     /locus_tag="PLP58_06720"
     CDS             complement(17185..18075)
                     /locus_tag="PLP58_06720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ08244.1"
                     /translation="MKMPAWMFASVVLLPGFLSAQEDGFTQAATLKDRRINESSGLAL
                     SHKHPGVFWTHNDSGGEPCLFAFDKTGVTVAKVRLPGAVNFDWEDIASRKDADGVSWL
                     YVADIGDNMRMRPSVQVYQIPEPDLPADPAHEIESAEPRLWRGAYPDGRHDAESLLVH
                     PLTGRIHIITRSEDGRSGVYAFPEKLLEDEAMTLEKIAALGFPLRARPGKRPRDAGMA
                     TAAAYSDDARRVIISTYSFFHEWTARPDEPLADVLKRQAEVLTPPVLRQTEGVCYDAD
                     KLSLWFTSEQLPTPLYFMRR"
     gene            complement(18091..18849)
                     /locus_tag="PLP58_06725"
     CDS             complement(18091..18849)
                     /locus_tag="PLP58_06725"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009964587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HRJ08245.1"
                     /translation="MKQASAACILPIVLGAVVIFAACSSPSASYGSRSRVAQWESRYH
                     DARLGQVTPEQLLREVGIKVDLIPAGTHGRRIYRPMRPTYITIHSTQNYTGNAYNHAL
                     ALKRGALRATKRRGGNRIGYLTWHFTVQQDVAIQHLPCREQGEHADFDGPGNNKSIGI
                     EMCEHRGNDIGLTIDKTARLAAYLMHTYGIPLSHVVPHYHWPRISPHVRDPHKNCPHF
                     LMDGGRPGPTWQWFLRRVHFHHSRLVPGPARSLG"
     gene            complement(18930..24632)
                     /locus_tag="PLP58_06730"
     CDS             complement(18930..24632)
                     /locus_tag="PLP58_06730"
                     /inference="COORDINATES: protein motif:HMM:TIGR02604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HRJ08246.1"
                     /translation="MPSGIMRVIPIITFLLAVASLSAQPVFRAGVATVDISPTEFPRI
                     IAGGFLEGRGERLADKLFVRSFVLDDGKMKIAFAIVDTCMMEQALIDEAKGIAAKQCG
                     IPVDRMMVSATHTHSAPAAMGCLGTRKDTVYAKFLTPKIAEAIVAANAALQPARIGWG
                     SFDDWEHTHNRRWIRLPGKEVVDPFGQPTGRANMHPGYLSKDVVGPSGPVDPQLSVIA
                     LQTLDGKPLGVLANYSQHYFGTSPVSADYFGLFCKHLAAKMDQQGDGNGPFVCAMSQG
                     TSGDQMWMDYGAEKKTITIDTYASEVADSALKALQTVKYVDHAPLGMVEKTLELNYRV
                     PDEKRLAWARPIAAKIENDLPKNKEEVYAREALILHERQKTSVKLQAIRIGDLSIATL
                     PNEVYAITGLKLREWSPFKTHFNIELANGAEGYIPPYEQHKLGGYTTWPARTAGLEAE
                     AESKMLPQLSYALGILGTGVAREAKKEVGVYGHEIKVSKPSAHYSCDEIVSPKLHSPL
                     SFARSMRSDEWVHDALLVGGHAMYLPGVGSGLGYGVESALKLSPFSKDQSINRAVQVA
                     GGHIKADWSNLPVERASPKEPAPSAASIALWFWLGHESGASDRAGELINALGVSLKAH
                     QFLDHTVRLEFGDKVASASAEGSDSAKAETTFFADDWHFAVLIRDGENVRVHLDGSEK
                     PVLTGKAGKAANEVLFGQGLEGRLDEITIWDRVIEPSLIAKLWNISKVGEENAKRAVS
                     RAERKKRAQVQSSALLKAHENWSASMRFKNTKANNVSAVTAYLISRGPKGDHQAPGDH
                     LGIGGNYKDSSPGRLFVFNGNAASQIVRGTTVIEPGTWNDVKLERLGSRVKVTLNGKV
                     EIDAELPVTAPGAKELFFGKRCDDFAPLEGSFEKVAVSGLEPSANPLRTTQTTSSKLI
                     EAQKGSPTDSGKPTDWLLKKSAGLPESVGNHSPHSVPTTTQPLSPEESAKKWHVRDGY
                     RIELVAAEPVVLDPVAFDWDEQGRLWVIEMADYPLGMDGNGKAGGRVVLLEDTDRDGR
                     YDKRHVIVSDLSYPTGILTWREGVIVTAAPDIFFISPDGTKKVLYTGFSTGNQQLRVN
                     GLRWGMDGWVYCAAGAHHGGYNKGTQIECKLTGEKIDLGSRDFRFKPDTGEFDPQSGP
                     SQFGRARDDWGHWFGVQNSFPLWHYVLQDHYLRRNPHVIPPDPIHQLFPRNPPVYPAS
                     SMEKRFHSFDQAGRFTSACGIEVYRDVTLFNDGKTHAFTCEPFHNVVQHHILEDDGVT
                     FKAVRDPAESKMDFLASEDRWCRPVMVRTGPDGALWVADMYRYMIEHPQWLPQNGKEE
                     LLPHYREGDDKGRIWRVVKSSHLAPRDEHTASKRGEASGKGNVPFISTERDDYFASPN
                     GWLRDKAQMRALWKRAIESPKVDLNEDKTFVQLNWTKLSLSKLPKDEVYRLFDHPSAR
                     VRAQALQMAEKLEWKADEASALQKALTKLLDDQDAKVRLQLACTLGELKFEWAGDLLA
                     EVLNSAEPGSSLQGAALSSVLPHLERVCARADAKSFAMLLRCALATKNEKAIAALVTR
                     MDAQKGLEELLAVLDEKNLSLADLAKQVTDAKAREAVKKMAARLQQAAESIQTAPTME
                     SLTLLASDREHRERVKALLPELWAKSVDVGALAKVRQDGPANTPDSRSLATAPTEVLR
                     LVSKLQPQGGEQFLLEGWDQRTPALRVQILETLLSNDAWTLALLKRPEAKSADAAMRA
                     RLMKHTKKNIANLAEKVFADSTSASRAAVVEKFKPALKLPGDAARGKTVFASVCISCH
                     KLDGVGLELGPDLRSVAQHDAEKLLNSILDPSAIIEPGFMAYHCTLKSGEQLYGVIAT
                     ETSASLTLKMAGNITKSVLRSDVASLKSTGISLMPEGLEAAMTPQSLADLIAYLQKPR
                     "
     gene            24713..26032
                     /locus_tag="PLP58_06735"
     CDS             24713..26032
                     /locus_tag="PLP58_06735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009965006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRJ08247.1"
                     /translation="MSVFASHPILRLFASPQHLWRTVSLGLKSIWLNKLRSFLTALGV
                     IFGVASVVAMLAIGEGASHEAQEQIRRLGSQNIILESVKPPDGQGSTGETRSMIIEYG
                     LTMRDIQQIRQTVPGVSVVVPSRILTENIWNLDRSVDGQIMGVLPVYPEMRNRRVLQG
                     RFFTDLEMNEKLPVCVLNETAASKLFPLSTPTGKSVRVKGYYYKIVGILEDEGQRVGT
                     DGGPSSNNASAQLMLPYSTLMDQYGETFFRFRSGSFEAEKVEFHEAVVRVEDVNQVES
                     RANAIRHLLAKNHKKEDWRIIVPVELLRQAERTKQIFSIVLGSIAAISLLVGGIGIMN
                     IMLASVTERTREIGIRRAMGARQADIVLQFLVETVLLSGAGGIIGVVLGVSIPVAVEH
                     FAGVTTVVKIWSPALAFLISVLTGIGFGIYPARRAARMNPVEALRHE"
     gene            complement(26188..>28291)
                     /locus_tag="PLP58_06740"
     CDS             complement(26188..>28291)
                     /locus_tag="PLP58_06740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009965650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="helicase-related protein"
                     /protein_id="HRJ08248.1"
                     /translation="LARRSGILPVSCATGILPVAGETGQNARFTRDRRDACPTRIGLS
                     ATAAPLPLLAEFLVGPGRACEIVEARMERRRRVEVLSPLRRDPYPSAGYTAQRVMEDI
                     ARIVERSRNVILFCNTRSSTESITIRLKNALPKLAEKIEAHHASLDRDVRLEVEDRLK
                     NGELRAVVCSTSLELGVDIGSVDCVVMISTPKGISRALQRIGRSGHSIHTESHGILVA
                     TNVNDLMECIVCAEMTRATRLDAVRPLECPLDVLAQHLVGMAMEGGHTRESVLATVRA
                     AFPFRSLTEAELDRVLNYLEGGGRSLEKQYRENFGKIVWRDGRLGVPNQKVEREYLAN
                     VGVIHTEGMVSVLLGKRRLGQIEEGFLKRLKLGDIFVLAGRTVRLMETSVAEARVEDA
                     VGRLPTVPAWNANKMPLSSGLAREVALLRTELAARMDEPEGITDWLIERFEISQTNAE
                     AIVRHCRNQLRVSRIPTDDTFLIERFVEDREGADTERVHFFFHSLIGRSANDALSRII
                     SHRLKEAVGGNTLVTIDDYGFLLAVKSFQDLELDAWRELFATGDPERDMRAALEQSEL
                     VKWNFRGVAQTGLMVPRNRPGKEQRLKHLRWSAEILFRVLAEHEPDHPMLAQAYREST
                     HTFLDLPRAIEFLERAPRLDWHLVDVPVVSPFSFGIYASKIKEGMMLEDPEEAIERLW
                     REFERKTGRAGSRSGVPA"
BASE COUNT         5078 a         8965 c         8957 g         5291 t
ORIGIN      
        1 cgaatcctgc tgcattgccg ccgcctcgac catcaccgtt cgctcgagcc gatcatggtg
       61 cgcacggcgg catacagcag cgggatgtgg ttttcatcgc cgtcaggaaa ccgccgccac
      121 cagcgcacga aggccatctg caccacatcc tccgcgtccg cccgcgaagg gcagagctgg
      181 caggcgtaga gaagcagtcg cggagcgacc tgctcgaagc agcgtttcca gatgtcagcg
      241 gcgggggcca tgcgggggtt caaccgcgta acgacgcccg tgtttctttt tgtgtgccgg
      301 tctgaaaaaa cacaccgcca atcacaccgc cagttcgacg acatgcgcgg tcaccgcctt
      361 ggccagcccc tccaaattgt atccaccctc aagcacggaa acgaggcgtc cctggcagtg
      421 ctcgtcagcg gcttgtttca agagccgggt caaggcagcg aagtccgtgt ccgtgagacg
      481 gaatcgcccc agaggatcgc gctcgcgcga gtcaaagccg gccgagatca tgatgaggtc
      541 cggtttgaag ctctcgagcg cgggcagcag cttctcctca aaagccgcgc cgatttcctt
      601 catgcccgct cctgcggcca gcgggcagtt cagcgtgctg ccgcgcccct tgcccgcgcc
      661 ggtctcctcc gcatgacccg tgaagggata ccagggtgat tgatgcgtgc tgaaaaagag
      721 cacgctgccg tcctcataaa agatgtcctg cgtgccgttc ccgtgatgca catcccagtc
      781 cacgatgacc acctttccag ccccatgcct cgcctgcgca tgacgcgcgc cgagggcgat
      841 gttgttgaac acgcaaaagc ccatcccctg cgcgggcctt gcgtgatgcc ccggcggacg
      901 cacggcgcaa aacgctcttg agagcttgcc cgtcatcacc gaatccaccg cctcgatcac
      961 accgcccacc gcatggagcg ccgcgtcatg cgagccgggg ctgatctgcg tgtcgcccgt
     1021 gctgagcgtg tcgagtcctg ccgcgatctc ctcccgcacc agttcgatgt attggcgcgt
     1081 atggcagcgc tccagctccg gcggctcagc gggtcgtgaa acaatcggcc tcagccgcgc
     1141 cgtaagtcca gcctccgcca aagcgcgcgt caccgccgca tgacgctccg cgcattccgg
     1201 gtgcccggat cccgtgtcat gtctggcaaa gacggggtct tgaagaagtc cggtgctcat
     1261 gaggggttgc ggagtttgcc cggactttgg ctgttccagg gcaaaacgca atccatgcgg
     1321 caatcatctc accaggccgc catcaatccc gtgggaccga ggcggacctg tgtgacgcct
     1381 tcgggaaggc gaaaacgctg cggggaatct gaaatacgcg gcccccagag ggtggctttg
     1441 ccgtcggatt cccagcccag ccagatgccc tcctgctgaa tgacacgagc ctggctggaa
     1501 agacccgccg gtttgcttcc ctgaatgtct gactgcgggc ggccatccat gtgcgcgggg
     1561 aatttctccg cttcagccgg gagcagtcgt ctcagaccgg ctccaggctc gcgcggcagt
     1621 aaaaaagccg cgccgctttc cgcccacgcc acggcatccg tggcggtggc ggtgagacga
     1681 acgatgggtt cagccagcac acttttccgg tcgccaggga ggatcagacc cgctgtggag
     1741 cgcgggggcc agacgattgg cagtcctttg cctggctcgc gatcactcca gatgataatg
     1801 ctgccatcgt ggagcaaggc cgcgccgcca tcggcgatga ggaccgcgtc tttccagccg
     1861 gcgccggtgg tggaagccgg cgggtacagg gcgggaggga cgggatcaag cgaccagaca
     1921 tgaaccttgg aaaactgcgg cggcaatgcc ccgggtgagg gcggctcctg gcgtgtctct
     1981 ggtgacgaca ccggggctgg catgggcgga ctttcttttt tggcgggata agattgggca
     2041 gcctccggtt tcggaggctc ggatgcgggt tccgggagtt tgggctgcgg ctcccgcgca
     2101 gtcattggcg catgttgatc cggtttgctc tccggcgctt gcttgaagaa taccaaagcc
     2161 gctgcggcaa caaccacgca cacgatcagc aaggccgccc acgcacgcca gggagcgggt
     2221 gccttgctcg tatccgcggc ggggcggctc gcggattctg tgacatcctc tttcaactct
     2281 gccgccgcct ggtagcggtc ctcgggacgc tcctggagag cgcgccgcac cacggagtcg
     2341 agccgcgcgt cgctgccagc cttgcgcgaa ggcggctgga agacgccgcg cggcagctcg
     2401 ccggtgagca tttcatagag cagcactccg aggctgtaga tgtccgcccg gtggtccgca
     2461 tgagcggcat cccgcgcctg ctcgggcgcg gtgtaggcgg gcgtgcccat gaaagctcct
     2521 gtgcgggaaa gtgtcagcgc atcctcctcg gcggcatggt ccagacgggc gaggccgaag
     2581 tcggccactt taacctgccc tgcggagttg atgaggatgt tcgagggctt gatgtcacgg
     2641 tggatgacgc cctgcccatg cgcgcaggcc agcgcgtcgc agacctggcg gacgacctcg
     2701 atggcgcgcg gcgcgtccat ttttcccccg gcgatgaggc ggctgagcgg ctggccgtcc
     2761 acatactcca tgacaaagaa gaagtgtcct gccggcgtga tgcccgcctc atgcagggcg
     2821 acgatgttcg ggtgccgcag gcgggccagg gcgcgcgcct cgcgccggaa acgctccacc
     2881 tgccgctcgt cagcgctggc ctcgatgggc agcagcttca ccgccacatc gcgggccaga
     2941 tcagactggt ggaaatgata caccgcgccc atgccgccgc gccccagcag gccctttgcc
     3001 tgccactgcg gaaaaagcgc ggccacctcc tccatgcctg gcggctccca cgctccacct
     3061 gccgccgcag gcgcggcctc gtccgccaga gagaagagcg cggcgggatc gaggccgtga
     3121 aggagcttgg aatcctggga tggcatggct cgcggggtca ccggtttgac caaaaggtca
     3181 gcgcgccacc aattccaccc ggcgattcag agcgcgtcct tcctccgtgc tgttcgtggt
     3241 caccggggcc atgaagctgg cgccgagggg cagcatgcgg tcggcggcga tgccatgctt
     3301 ggcggcgagc tcggcgacga cggccctggc gcggcgtttg gagaggtctt cgttgaactc
     3361 gaaagtgccg acgttgtcgg tgtggccgac gacgaggact ttcaggttcg gctcggcttt
     3421 gagcagcgcg gccatctcgg cgagcgtggg ggcggactcg ggtttgatgt cggccttgtt
     3481 gaagtcgaag aggatgccgt agatggccac cttgccgctg gaggagatgg atttccgcat
     3541 ctcggcggcg gagaggtgca ccatcttttc ctccatcggg cgtgtgttca cgacatccac
     3601 ctggatgacg acggaatctt gcgggatggt gatgtgctgc ttccaagggt tcgcctccga
     3661 gctgggcagc atggggtgat cccacgaacg cgcggcgatg aacacggcgg cgaaaaggcc
     3721 gctcttttcc atgcagaagt agcggcgctc ctttaaaccg gtggtcatga gctggtgatc
     3781 cagctccggc acgccgtagt aagcgcgagg cgggccgttg cggatctcgc ggtcgccacc
     3841 gctccagatt tctgcgaagc cggcgtcttt gaaggcaatc tcgtagttgc gggcgatctc
     3901 gagcgtgctg cgcttaccgg caccgaggtt caaataaaga taagtgctgc ggtgcgtgcg
     3961 gccttcgacc ttgcgcacct ctttcggatc cgtggagacc ggagaactga ggccgccgat
     4021 ttgcaggccg agttcgccga atttctgctc ggattgcagg acgatggtgc tgccttcata
     4081 gcgtggcatg ccgggatagt ccttggtgtt cggagcgtcc ttggtgggca cgagcttctg
     4141 ctgcgcaaaa gcaccggttg tggcgaggag gaggaggagg tgatgaagtt tcatgggatg
     4201 aacggggggg tcgacaggct ttctggcggt gcgtggcggg atgttacaaa aaatcatttc
     4261 cccagggctg acttcgcctg gtcaacaagg tactttgggg cactgacggc aggattcttc
     4321 gaggaataga cgacttcaat cggcccaccc tcgtcatggc ggccgtaagt gtcccaggtc
     4381 accacagatg tgtgctcatg cgccactccc gcgctgtcgg tgtagcgata gacgagcttc
     4441 tggcggcgcg aggtcgaact gccggctttc accgaacgct tgccaatcac ttggccgctt
     4501 gtctcaatgc cgtgctcgca aagctctcga aattggaggc cgcgtttcac aagcaagacg
     4561 atgatggcgc ccacaatgag cacgggaacg aggagaccaa tgagaattcc gatcatggtg
     4621 catggatggg ttgaatccgg cgaaacttta caagggcttc gtcgttcgtc attctgaatt
     4681 cgtcatttgg gccgcaccca ttatgttccc gcatgaccac cgcctcaaac gccgcctttc
     4741 ctgtcacgcg ctggagcctc gtggccggcg cgcgtgccgc ggcggacccg aaccgcaaac
     4801 gcgcggcgct ggaggagctg tgcgggctct actggcggcc gatctacgga ttcctgcgtc
     4861 gtcgcggtct gagcgtcgcg gaggcgcagg acgatgcgca gggctttttc atgtcgctgc
     4921 tcgaagaaga cctctttgcc aaggcgcagg ccgagacggg ccgcatgcgc agctttctgc
     4981 tggctgccct ccaacgttgg cagcgcggcg agtggcgcaa ggcgcaggcg gagaagcgcg
     5041 gcggtggcaa cgagctgttc tcactcgaag tcatggccga ggaggatggc ttcgaggccg
     5101 ccggcaacga cgatgacacg cccgagcgcc gctttgaaaa aagctgcgcc ctcgcgctcc
     5161 tcgaagccgc gatgcaacga ctcgccaaag aacaaagcgc cgcggggaaa ggcgaaaact
     5221 ttgacacgct gcgcccgctg ctggctccgc tcggcggcga gacatccatc acgcaggaag
     5281 aggctgcgaa gcgtctgcgc atgacggcag aggcactgcg cgtgactttg caccgcctgc
     5341 ggaaacgttt tgccgaggtc ctgcgtgaga ctgtggccga cacactgacc gatccgacgg
     5401 agcaggcgat tcaggaggag atggatgctc tgagagcggc tttttaggct tggaaaaaaa
     5461 tttcgcggaa cgtgtaacgt cctcccgcgc cctgccagac aggggatgac gcggccctct
     5521 ttgcggtcgc aagaactcaa accaagtcat ccccatgaaa acgaaactca ccctcctcct
     5581 cgccctggcc gtcagtgctt cggcgaacgc cgaaaagccg aacttcaacg gcttcaagcc
     5641 cgcccagagc gcctccgctc cgcgtgccgc gccgtcttat tccggtggtg gctcgtcccg
     5701 ttccttcaat cccggccagg ccatcggcat cgcccgcagc ctgctgccgc tggtcatgaa
     5761 tgccacgcct cctccgccgc cgcgcccgag cgtggtcaaa cgcagcccca gcccggccgt
     5821 gaccacgaac aagcccgcca cgcgcagcgt gtccaaaccc gccacggtga cccgcaatcc
     5881 ggcgtctggc agccgtggcg cgcttcccgc cccggtgggc ggcatggacc cgaaactcgc
     5941 cgagggcctg gaagccggcc gccagatcgc gagcggtctt cagtcacttg agtccctgcg
     6001 tgcctctttc ggctccgtgg aaggtttggg tgatgtgttt caccagcccg gcaacgccgg
     6061 accaggcagc cagggcggtg tgcgcgatcc gtttggcggc ggttctgaat atgatggcgg
     6121 caaggtgagc aatccgctcg accgtttctc caccgccaag ccgcgtgaca ttcgcggcgg
     6181 cttcagcaac agccgcaggg ggaacgcaac aaccggcctg agccggacag gcgaccgcgt
     6241 tttggagagc gaagtctcca cggcctcggg acgcgatggc accagcacgc ctgtgcaatc
     6301 caccacgcac gaaaacggcg acgtcactgc cagcaccacc gccaccacac gcgacggcaa
     6361 tagcacggag gtggaggtca ccgaattcag cgatggctct ggaacctcga ccaccaccca
     6421 cagcaacgac ggcaatgcgg acaccctgat tgtgacccgc cgtgacagct cgggtgccgt
     6481 gctttggaca agaacggaca gcgtccatgc cggcggcaag cagcacatcg tgacccgcga
     6541 tggcagcggc agggtcacct ccgacaccac ggagtattac acaccgggca gccgcacctg
     6601 cggccgcagc cctggcgaaa tcgccgctgg cggccccgtc aacggcaccg gccccggtgt
     6661 ggctgatgcg gccggcctga tgcccctcga cctcctgcgc cagttcgcga atggtgaagg
     6721 cgccagcgga gggaccaaca ccatgaccag cggcagtctc agcagccgtc acgtcaaccc
     6781 cgaccgcaac ggcagcagcg gccccatgcg caaccgcctg accgtggaca cctttggcac
     6841 catcagcaat cccgctcccc tgggcggtga aggtgccgct ggcggcggca acaggcctga
     6901 ctgaaccggc ccggcaaaac cgacttaaat gcccctgggt gcctcgcatc caggggtttg
     6961 gtgctgcttg agaccatcta tcgcaaaggg gcgctccata aatttcctcc cgaggtgatg
     7021 aaaagcgttt tgccatccgc tccaccgaag gcgcagttgc agcactcgtc cggcgcggtg
     7081 ggaacaaagt cgagcagacg gcctgagccg gagagtatgt aacagcccgc cttgtagcga
     7141 gtcgtttcat attcggtggg tttgttcgtg ccggcagcca cataaatgcg cccctttgca
     7201 tccatcttca tgccgtcagg tccgcgcccg tctttccagt caaagatcac cctcctcgtg
     7261 ccaggcttca catcgccata ttcgtccagc tcatagcgca gcagtttgcg cgagccgccg
     7321 cgggtgttgt tgttgttgtc ggcgatgtag agaaagcggt cgtccggggt gatgaggatg
     7381 ccattcggcc gctccgcgcc ttcgcagagg atgcgggtga catttgggat gtcatatgcg
     7441 aacgcacaaa gtccgtccgg aagcctggcc tgccgattga tgcgatagac gccttcgatg
     7501 ggcatctgcc ccggcttgat tcctgagggt tcgcacccgt caacttgatc aaatatttcc
     7561 atccccgccc gcgagccata acgcgggtcg gtaaagtaga tgagaccctt cgagtccatg
     7621 cacaggtcat tgggagagtt gaagcgcttc ccgagaaacc gatccgccag cacggtgatg
     7681 gagccgtctt tttccgtgcg cgtcacccgg cgcttcttcg tctcacaggc cacaagcctg
     7741 ccctcgcggt caaagagcag tccgttcgag gcattgtggc ggaaaacact gacgcgcccg
     7801 tccggtccca tgcgattgat gtgcttgccg tccgtgaagt aaagcgagcc atccttccac
     7861 gccggcccct ccaccgcgcc gatgctgccg tggtccctgg ttttcgctcc cggcaccaga
     7921 ggggatgtca gcacggtgca ggaagaaagg gcgaggggca gcaggagcgg caggtatttc
     7981 atgacacgcc atcaaacgcc cgtcacggca ttgaatcacg ccaaaaataa actgcccagg
     8041 catccagaac gcggacctca catgcggcgg cccgcaagcc tgcgcatgag ttccttggcc
     8101 tgctccacac taaggtccgg ctgcaaggaa accagccgcg ccagccaggc gctgaccacc
     8161 ggggccgcga agctgctgcc tgtgactttt ttccatccgc cctgccaggg aacggtcacc
     8221 tcgaaaccgc gcgcggcgaa ctccaccatg cccgcaccgg tccggtgctc ccagccgtcg
     8281 tggtcgagcg cggccaggtt caccgtcagc accgtgggaa accagccggg ccactcgcgc
     8341 aggccggcgt cgtcgttgct gcacgccgcc acgatgtgcg cgcggctcag gtaagcggca
     8401 tccgtccact ctttgtaggg catgacaaag cgcgcctccc cagggctgcc aaaactgcag
     8461 tttaaaatct ggtagccgcg ctccaaagcc agccgcgccg cctcccaaat gacggtgctg
     8521 cgcgaacgca gggcgccatc cagcacgcga aaactgccga tctccgcgca cggagccagc
     8581 cggcggatga tccaggcgat cgccgtgccg tggccaatcg catccccgtt gccagccttt
     8641 gattccaaaa aatttccccc cgcctcaaac atcacatcat ccgccaagct caggccctcc
     8701 aatgccggat gtgcggtatc cacacccgaa tccaggatgg cgacacgaac acccgcgccc
     8761 gaactcgcgc ccaaagcgat agcggttcgt gcaagcgctt cctcatcatg gaaataactg
     8821 ctcatagaga aacgggggtc agtcctccca gccgagcgcc caggcactga ggcggtggtc
     8881 gagcagcctg ccgagcacct gcgcggcggt ctccatcgcc gccagatgag ccaggctgaa
     8941 ggactccggc agcgcatgac caggcggcaa gtcagccctg gaacgcggcg cctccctcaa
     9001 gccgggcagc gcgtcgagcc ggatcaagcg cacggcggtg accacgccgc gcacctcccc
     9061 cgcgaagcgc agcggcacgg ccagcatggc ccaggtgagc acgccgagcg cgcggtcgag
     9121 cgcgcggttt tgcctttgat ggaagcacac ctcgctctcg cacatggcca gcccgctggt
     9181 gaagaccatg ccggtgatgc cctccgccgc cggcaggcga aagctgccgg tgaaacgcgc
     9241 cgcgtccggg ccgttgttcc agaccggcac cagcaccgcg ccatcctcca ccgccagcca
     9301 gagcgatccc tcatgcgctt cgatgttttt cagagccgcg tccaggcccc gcgcgggcag
     9361 cccctccgcc agcgcggccg cgcgcgtctc ctgcgcgcgg cgcaggaaat ccatcctgcg
     9421 ccgcgaaaaa cgctcctcga agtcggcgag atcaggatca ggcaatgcgg gaaacggcgc
     9481 gggcatgaga gcgccatcat gggcggaaaa cactggaagg acaaccacga gtccggggtg
     9541 cccgccatcc ctcacacaag ccgcagcgtg tggtggaaca actccccgca gaatgcctcc
     9601 aacctcagtt tccaatcaac cgcgccaatc ccgaaagaat gtccgtctca ttcgccttct
     9661 ggccgggggc gggttctttg gcgaggagga agtaaccatg acctgcggag gcgccggggc
     9721 ggttcacgat catgccggta tagctgaccg tgcgcggcgc ggcgccgatg gcggagggca
     9781 cggtgagcct gccgctgaac aggccggtct tgctgacgag cttgatgctg actttgcctt
     9841 cattctccgc agtgccagcc ttgggcacac cggcgttgtt cacgattcca gtggcacttg
     9901 ggttggcgag ggtcacgagc acatcggggg cttcgccctc gctgtgagtg aggccggcgg
     9961 ctgagaaaag cagcgcggca ttgttctgcc cggcatccaa ccccatgagc aggccgccag
    10021 gcgcgggcgg cgcatggatg ccgccattga cctcgacccg atcagggccg aaaccggctt
    10081 tgtaattgcg ctccttgctg ttaggcaaag gctcgtcttt gaaccagtcc ggctccaaat
    10141 cttgcaaggc gccgatgaag cccccgctga tctccagcgc tccggccagc gagccgcggt
    10201 ttttgtaaag ccccgcgtag agcagcacct cgccctgctg gccgacgaaa gtggagcagg
    10261 tgtaggcgct gccatcggca agcttgccgg cgactttgac gacaccgttg tcaggcacgg
    10321 tgaagctgcc gtagccgtca ccctgcggga tggcttcgat gccggtgttg gccgctgccg
    10381 gttcgatggc aaaggtgtag gcggctttga aagcggtggc cttctcgtct ttggtccagc
    10441 cgttgcgcca agcgctgacc tgggtgctga taccggggct gtccttttca ttgagcgtgc
    10501 cggagagcga gttggtgtcg ggatcgaact ccacatcgag cacgagtgtg gacagcttgc
    10561 cgcgcttgat gtcggtgacg agcttcggtt ttttcacgat ctcagtgaaa gtatcgagcc
    10621 ggccggtgaa ggcgtggctg acggtgccgg tgaggatttt tccgctcacc acgccggtct
    10681 tggcgacggt gagttcgacg cggctgccga ggccgttgtt gaggttgctc aagcgcccgg
    10741 ccaggccgtg aaacacaccg atgtggcgcc cgtcgaggtc ggtgacctgc aggctgaagt
    10801 cgagcttttg cggcgcggct ccggcggcgt tggcggcggt gatgctgaag tcgaattcgc
    10861 cgggcgtggc gggcttgcca gagagggtgc cagtcagttg gttgaagacg aggccgggag
    10921 gcagcttgcc cttgatggtg tacttcacgg gcggattcac cgtgggaatg gtcatggaga
    10981 agtcagcgct cacagccagc ggggcgaccg ctgcgggaaa gtcaatcgtc ggcggcaggg
    11041 cggcggcctg cgtgagggca tgcacggctc ccgcgatggt gatcttggct gtgcgggctt
    11101 ttttggagtt gttcggctgc acgttgacgg tgacggaccc gttgccagtg ccggatttag
    11161 gattcaccac agcgaaggac gcggaggact tggcagtcca tgcgccgggg gtggtgacag
    11221 cgatggtgta gctttgaccg gcggccgcga tgctgttttc caccggatca agcacctgcg
    11281 ccgcgccggc gtcagcaggt gtgatcttga agctgcggat catggccatg tgcccggaaa
    11341 actggccggc gaagccgtag atgcggaact cgaccgggcc gccgcggttt tcaaaggcgg
    11401 cggtgtccgg caggcggaag gcgaactcct gctcgttggt gtcgccatcc agccgtgtcg
    11461 tggtaaagac cgcctgcgcg gcgctgaacc cgcccacgct ggtgagcacc gcatgaagac
    11521 gcggcgcgtg aaagctgagc ctgcggatgg tgaaacgcac ctccgcattg cgcaggttca
    11581 gcggcgagcc tccctggacg gataccgccc aaaattgtcc ggccgtgatg gcctgcgcga
    11641 gtgtggactc gttgttcggc atcacttgcg agtaggccag gccgtcgtct ccggcctcgg
    11701 gcgtgatccc cgcgcccgct gtccagccgc tgtaggtgac gccgtttttc agcacatagg
    11761 tcggagtcca gggcaggttc agcgcggggt cgctgccttc gaaattgctt tccgccagca
    11821 cgccggggcc gccgacggtg cggaagaaaa aggtgcgcca ggcagggtcg ccatcggcat
    11881 cccgcacgcg cagataaagg aagctctccc ctgtgctcgc gggtgtgccg gagagcgtgg
    11941 cctcgcctcc attgagcgtg atattcaggc cctgcggcag caccgcgccg atgatcttca
    12001 gagcgcgcgc tccgtcgccg ccagtggtgc tgatcgtggt ggtgtaaggc gtgccatgga
    12061 tggcggtggg cagctttgcc gcggtggcaa aggcaggcgc actgccgagc acatcgtcta
    12121 tgtggcgcag cccggccagc tccgtttccc aatcaagaag aaactgccac ttcggcgagt
    12181 cctgcgtgct ttggcgaatg ctctccaggt ggccccattg gccgaacttc ccccagccgc
    12241 ctgactcgac gaagacgccg tggctgcgca ggcctttttc gagcgcctgg ttgagatgaa
    12301 tctcatacac gtccgccatt tccggctgac ggttcaccag ctccatgaag gccgtgatgc
    12361 catcgtcgcg ctcatcaggg ccgtccagtt catccgtgaa aagcgagggt cctccctcat
    12421 aggagacaat gggtttcggt gcggtaaaaa gcgtctgcgc cagcgcacgc aagccgtcgc
    12481 caaagccgcc gccgatgccc gtggcgtcag ggccggagcc ggtctgcgtg gagccggaga
    12541 gcatgcgccg ccgccactcc tgaaacagct cgtccatgtg gcggttgatg gcgtcactca
    12601 cccagtaacc actctccggc gtcacgctca caggtcgctg gccgccgtca aagttgaaat
    12661 tccccgtcgt gagaaaccag gggtcgatgg tggcgcgctg ctccaaggca cgctggaaag
    12721 caaagccctg gatgtcattg ccgaagtagg tggtgggcga gatgcagtcc ggctcctgcg
    12781 gcggggtgag cgtggcgccg tggtctttga tctcggtgag gaattcctcg atgtaacttt
    12841 ggctgccggt gaagacggct gcggtcttaa tgaggcggtc attgccctca aagacctgct
    12901 caaagacacg ccacacgcgg caaaactggc gagcgttgaa ctgcggacgc gtgaggccct
    12961 gctgcgcggc tttggcctcg gcccagttac cctgcgggaa gctgaagccg ttgctccaga
    13021 tctcgttcga gtactccaca tagacgcgca ggttcgggtt cagcggcgcg aaggcgggat
    13081 cggccactgg cgcggtgtgc gggttcacac catcgctgcc gaagcggatc atctgcgcca
    13141 gcttggtgat gaaatcatcg tccgccaagt gcgggatgca gatccacagg tccttgttcg
    13201 cagcgttgca gagcgcgatg atgtgctcgt aggccactcc agtttcacgg tcgccgatgc
    13261 tgagggcgaa ctcgtccgtg gggtcgcggt cattgaggac gccgcattga aagacatggt
    13321 ccggcaggcg gcggtcgctc cagcttttct gcgggttcgc attggtcacg ccccagtcca
    13381 tcatgcgcag gaaggaccag tccacctcgg ccaggcgttg caaaaagaca gggtgaaaaa
    13441 gctcgtcctc cagcgtggca ttctgcggat cggcggggtc aggcagccac accttgaggt
    13501 cggtgatggg gttcgccggg ttgatggact ccacctgcat gaaggaattg tcactcgcat
    13561 tgctcttcag atagacacgg cggccgttca ccaggcttcc ggtctccgga ccgttcgact
    13621 cgccggggat aaagacgccg tcattgagcc ggatgtcggc atcgccggtc cactcgacga
    13681 cgatcttgcc attcgccagc acgctgtcac gcaccggcca ggaggggtgg cgcagatcgc
    13741 catagtcagt gtgcaggccg aagagcagca ggcgcagctt cttgccgggg ttgaggaatt
    13801 tcggatagcc tttttcattg aactgcgccg agttgaagtg cggcacgcgg tcgccgaagt
    13861 tcgtgtcgaa ctccagccac tcgccgccct gggccaccgc gttggcaaag tagggcgtgg
    13921 taaagtagtt caccccgccg acattcacac cgagcggttt gagtgtggcg gcgtcgaatg
    13981 ccgtggcggg cagggcggcg gcaagcagca gagagagcgc ggcggagttt tgcaggaggc
    14041 ggaggagcgt tttcatggga tgcgtggatg tggcggccaa gcggccttca ccgggtggat
    14101 gcgcgaggca tggggtactt gcgcaactaa cgcgcaaagt ttcaaaaaaa cggccgacgc
    14161 aaacgcgcgg accgcccttt catggcaaaa cccgaccggg agattccgaa agctggagtc
    14221 gttccatcca ggatgaccat gaagctcctg cccgccctct tttgcctcgc gccgcctgtc
    14281 tgcgcggatg attccgcccg cctcgccgaa ctgctcgcca ggggcggtgt ggtaacgatc
    14341 ccccccggcg actatcaact caatggcgcg cagccgatcc tgctggtgtc gaacacgacc
    14401 gttttagctc atggcgcgcg ctttatcctg cccgaaacac tgccggatca ggcgcggctc
    14461 atcatcttcg cgggacaaga catcggccat ttcacctggc agggcggcga gttcatcggt
    14521 catgtctttg atcccgcgcg gcgtgaaaat gcctggccgc caaacgcaaa cacgaaaggc
    14581 atcgagatca ccaccacacg ggacggcggc acgcacggca tccattttcg tgatgtgaaa
    14641 tccaatggca tggcaggcgc cgtcatcggc gtgcatggcc tggtgaaaaa aggcggcgag
    14701 aatgaggtgc tggcccatgc cgagcgtgtg gcggtggaga gctgcacgct tttgcgcagc
    14761 ggcaaattca tgtgggacta cggctacctt tggcagatca tgacctggcc ggaggaatat
    14821 gagagctggg aggtggagcg cgcgcggcaa tacttccgca ccgatctcat tcgagaagtg
    14881 gagttcatct cgggtgggga tcgcgtgctc tttgacaaca ccaaccgccc cctcgccgtc
    14941 agccagaccg actcgccgca ggaggccatc accttcatgg gcgcggacct gccgccgggc
    15001 attgagcgcg ggcggcaata cttcgtcacc gagtccacgc cggaattcat caagatcgcc
    15061 aaccagccgg gcggtcaggc ggtgaggttc ggtggcgacg gcaaaggcag cctcgttttt
    15121 ttcatccacg ccgcctatct cagcgcctac gcgcccaccg gcagcggccc cggcaagggc
    15181 gccttcgaca tcaccggcgc gcgcgatgtg cgcgtgaccg gctgccagct cagcgccctc
    15241 ggtgacacga tgcacatcca ggcctgccat aacattgtct tctccagcaa ccacatcctc
    15301 ggcagccgca tgggcgcgtt tttcctggcc gagttttgcc agaatgccac catcaccggc
    15361 aatctcgtgg acggcaccaa cggctcgcgc gtgatgagcg tggagaaaag ctgcacagat
    15421 gtcaccatca ccggcaacac cttccgcaac ggtggacgcg gctcgtggat caaccagccg
    15481 gtgaacttca tcatgaccgg caacgtcttc gtcaataaca ccaccaaaaa cgagaccgac
    15541 atgcgccgcg gccgcatcgc ctaccagacg ggccttccgg tgcggttccc cgagctttac
    15601 ttcaccctcc acgagcctga tgccgcttac ggcccagtca tcgtgcgcga caacatcttc
    15661 cgcctcggag actctgcgcc cgaggaggcc gtgaccttcg cgccgaacgg gaaggacgtg
    15721 cagttcacag gcaacatctt ccaaaacaag cccgccatca ttgtcgtgga cccctcgtgc
    15781 tccggcacgc gtgtcgagga caaccccggt gccaccaccg tcagcaggcc cgtggatttc
    15841 aaccatggcc ggaggtgact ccggacttcc cgtgcggcat tctcagcctt caggttccgc
    15901 gcttgttgcc gaacagctcg atgtgcaggc gcggacagaa gcgccatcca tgatccttgc
    15961 aggcattcac aagcaggtcg tagcgtctcc tcatcgcctc catgtctgtg ccctccggca
    16021 tgagaaggat tttctccggc ggcactggcg cgccaatggc ggagatcacg ccctgcatct
    16081 cgcgcaagtc tgcctcattg gagatgacga acttcaactg ataatcatcc gcgctttcca
    16141 tccactggcg caccacctcc ggacgcaggc gcgtgtgttc gtgcctctcc acccacgcgg
    16201 tccccgtgct ggcgggatca gggcgcgagt tcgccagctt gggactgatc gaagccagat
    16261 tcacgcgcgc accctccggc ggcaccgtgc ctgctgtctc cagcgtcaca tgccttcccg
    16321 cctcgcgcag ccgggccagc agcacgcgga tgtcacgcgc caccatcggc tccccgccag
    16381 taacgaccac aaagcgcgcg ggatgctgtg ccaccctgtc aagaatttca tccacactca
    16441 tctccccgcc ttccggagac caggaggcat acggcgtgtc gcaccaggcg cagcgcagat
    16501 tgcatccgga ggcgcgcaca aagacggacg gcacgccggt gagggcgccc tctccttgaa
    16561 tggaataaaa gatttcggaa atccgcatcc gccagcatag ccgccgcagt cttcgtcaca
    16621 atgcccgcct tgccgcgcgc ctgccgcgca ttacactcgc ccatgcctca tttccgccgg
    16681 ctgctgcttt tcaccgcctt tctttgcgcc agttgcgcat cgaaaccaaa aatgtggcac
    16741 taccagtacg agaagggcag gacagccatc ctcgtgaacg gtcaggccgt gccacccgcc
    16801 gggctgccga aaaaagtcat gcaggcggtc agcgcgggaa accgcatcgc cgggctgccc
    16861 tacaagttcg gtggcggaca caggtcgttt gaggacaacg gttacgactg ctccggcgcg
    16921 gtgtcttatg tgctgcacgc ggcgggcgcc ttgaaaagcc ccggcacttc cgaaagcctg
    16981 cgctcctttg gaaaaaaggg cgcgggcaag cacatcacgc tctacaccaa gccggggcat
    17041 cactgcttcc tcgtcgtggc gggtcttcgc tttgacactg gctacaatgg ccagcgcaaa
    17101 ggccccgaat ggtccacaaa aagccggcct gtcacaggct acaccgcgcg gcatccaccc
    17161 gggctgtgag agcgcctcac tggatcaacg ccgcatgaaa tacagaggcg tgggcagttg
    17221 ctcgctcgtg aaccacaggc tcagcttgtc agcgtcataa cacacgccct cggtctggcg
    17281 cagcaccggc ggggtgagca cttcggcttg gcgtttcaac acatcggcca gcggctcgtc
    17341 agggcgcgcg gtccattcgt gaaaaaacga gtaagtgctg atgatgacgc ggcgcgcgtc
    17401 atcggagtac gcggcggcgg tggccatgcc ggcgtcgcgc gggcgcttgc cgggacgcgc
    17461 gcgcagcgga aatccgagcg cggcgatttt ttccaacgtc atcgcctcat cctccagcag
    17521 cttttctgga aaggcgtaca cgccgctgcg gccgtcctcg ctgcgagtga tgatgtgaat
    17581 gcggcccgtg agcggatgca cgagcaggct ttccgcgtca tggcgtccgt ccggataggc
    17641 gccgcgccag agccgcggct ccgcgctttc gatttcgtgc gccggatcgg cgggcaggtc
    17701 gggctcgggt atttgataca cttgcacgga aggccgcatc cgcatgttgt cgccgatgtc
    17761 agccacatag agccaggaca caccatccgc gtccttgcgc gaggcaatgt cctcccagtc
    17821 aaaattgacc gcgcctggca gcctcacttt ggcgacggtg acgcccgttt tatcaaaggc
    17881 gaacaggcag ggttccccgc cggagtcgtt gtgcgtccag aacactcccg gatgcttgtg
    17941 gctgagcgcg aggccgctgc tttcattgat gcgccggtcc ttgagggtgg ccgcctgcgt
    18001 gaatccgtcc tcctgcgcgg acaagaagcc gggcagaagc accacggagg cgaacatcca
    18061 ggctggcatt ttcataaaac gcatgagagt tcagcccagc gaacgggcag ggccgggcac
    18121 caggcggctg tgatgaaaat gaacgcggcg aaggaaccac tgccaagtgg gccccggacg
    18181 gccaccatcc atgagaaagt gcgggcagtt tttgtgcgga tcgcgcacat gcggactgat
    18241 gcgcggccag tggtagtgcg gcacgacatg gctcagcggg atgccgtagg tgtgcatgag
    18301 gtaggcggcg agacgcgcgg tcttgtcaat cgtcaggccg atgtcgttgc cgcggtgctc
    18361 gcacatctcg atgccgatgc ttttgttgtt gccagggccg tcgaagtccg cgtgctcgcc
    18421 ctgctcgcgg cagggcagat gctggatggc cacatcctgc tgcacggtga aatgccaggt
    18481 caggtaaccg atgcggttgc cgccccggcg ctttgtagcc cgcaaagcgc ctcgcttgag
    18541 cgccaatgcg tgattgtagg cgttgcccgt gtagttctgc gtgctgtgaa tggtgatgta
    18601 ggtgggccgc atcggccggt aaatgcgccg cccgtgtgtg ccggcgggga tgaggtccac
    18661 cttgattccc acctcccgca gcagttgctc cggcgtcacc tgccccagac gcgcgtcgtg
    18721 atacctcgac tcccattggg cgactcgtga gcgcgagccg tatgaggccg agggagacga
    18781 gcacgccgcg aaaatcacga cagctccgag gacgatgggg aggatgcatg cagccgaggc
    18841 ttgcttcatg cgcgcatgat tgcctctttg tccgtttttg ccagcgtgga gtctgaaagc
    18901 atccgttaaa atttcattcg actcatgctt caacggggct tttgcaaata cgcgatcaaa
    18961 tccgccagcg actgcggcgt catcgcggct tctaggcctt cgggcatcag ggagatgccg
    19021 gtgctcttca aggaggccac atcgctgcgc aggacggatt tggtgatgtt gccggccatc
    19081 ttcagcgtca ggctcgcgct cgtttcggtg gcgatgacgc cgtagagctg ctcgccgctt
    19141 ttcagggtgc agtggtaggc catgaaaccg ggctcgatga tcgcgctggg gtcgaggatg
    19201 gagttgagga gcttttcggc gtcgtgctgc gccacactgc gcaggtccgg accgagttcg
    19261 aggcccacac cgtcgagttt gtggcaactg atgcacacgc tcgcgaaaac ggtcttcccg
    19321 cgtgcggcat cgcctggcag cttcagcgcc ggtttgaact tctccaccac agcggcgcgt
    19381 gaagcgctcg tggagtccgc gaagaccttc tccgcgaggt tggcgatgtt tttcttcgta
    19441 tgcttcatca accgcgcccg catcgccgcg tcggcagatt tggcttcggg ccgtttcagc
    19501 agcgcgagcg tccacgcgtc gttcgagagc agtgtttcga ggatctgcac gcgcagcgcg
    19561 ggcgtgcgct ggtcccagcc ttcgagcaaa aactgctcgc cgccctgcgg ttgcagcttc
    19621 gagacgagtc gaagcacctc tgtaggcgca gtcgccagac tgcgggagtc cggcgtgttc
    19681 gcggggccat cctgccgcac tttggcaagt gcgcctacat cgacgctctt cgcccaaagc
    19741 tccggcagca gcgctttcac gcgttcccga tgctcgcgat ccgaggccag caaagtcagc
    19801 gattccatcg tcggagccgt ttggatcgac tccgcggcct gttgcagcct cgccgccatt
    19861 tttttgaccg cttcacgcgc cttggcatcc gtgacctgct tcgccaaatc cgccagcgag
    19921 aggtttttct catccaaaac ggccagaagc tcctccaggc ctttttgcgc gtccatccgc
    19981 gtcacgagcg cagcgatggc tttttcgttc ttcgtcgcca aagcgcagcg cagcagcatc
    20041 gcgaacgatt tggcatccgc acgagcacaa acccgctcca gatgcggcag caccgaactc
    20101 aacgccgcgc cttgcaacga cgaacctggc tctgcgctgt tcagcacctc cgcgagcaaa
    20161 tctccggccc attcgaactt cagttcaccg agcgtgcagg ccagttgcag ccgcactttg
    20221 gcatcttgat cgtccaggag cttcgtgagc gctttttgga gtgcggacgc ctcgtccgcc
    20281 ttccattcga gtttttcggc catttgcaga gcttgtgcgc gaacacgtgc cgatggatga
    20341 tcgaacagcc tgtacacttc atctttgggg agcttgctca aagacaactt ggtccaattc
    20401 aattgcacga aggtcttatc ctcgttcaaa tcaactttgg gcgattcgat ggctcgcttc
    20461 cacaacgccc tcatctgcgc cttatccctc agccagccgt tcggcgaagc aaagtagtca
    20521 tctcgctccg tcgagatgaa cgggacgttt cctttgccgg acgcttcccc ccttttggac
    20581 gccgtgtgct catctcgcgg agcgagatgg ctacttttca ccacccgcca aatacgcccc
    20641 ttgtcatccc cttcgcggta atgcggcagc agctcctctt tgccattctg cggcagccac
    20701 tgcgggtgct cgatcatgta gcgatacatg tcggcgaccc acagcgcgcc atcggggccg
    20761 gtgcggacca tgacggggcg gcaccagcgg tcttcgcttg cgaggaagtc catcttcgac
    20821 tcggcgggat cacgcacggc tttgaaggtg acgccgtcgt cttcgagaat gtgatgctgc
    20881 acgacgttgt ggaagggctc gcaggtgaag gcgtgcgttt tgccgtcgtt gaagagcgtc
    20941 acatcgcgat acacctcgat gccgcaggcg ctggtgaagc gtccggcctg gtcgaagctg
    21001 tggaagcgct tctccatgct gctggcggga tacacgggcg gattgcgcgg gaagagctga
    21061 tggatcgggt ccggcgggat gacgtgcgga ttgcggcgca ggtagtgatc ttgcaggacg
    21121 tagtgccaga gcgggaaact gttctgcacg ccgaaccagt ggccccagtc atcgcgggcg
    21181 cggccgaact gcgaagggcc gctctgcgga tcaaactcgc cggtgtccgg cttgaagcgg
    21241 aaatcgcggc tgccgaggtc gatcttctcg ccggtgagct tgcactcgat ttgcgtgcct
    21301 ttgttgtagc cgccgtgatg cgcgccggcg gcacaataca cccagccgtc catgccccag
    21361 cgcaggccgt tcacgcggag ctgctgattg ccggtgctga agccggtgta gagcaccttc
    21421 ttcgtgccat cgggcgaaat gaaaaagatg tccggcgcgg cggtgacgat cacgccttca
    21481 cgccaggtca aaatgccggt gggatagctc aaatcgctga cgatgacgtg gcgcttgtcg
    21541 tagcggccgt cgcgatccgt gtcctccagc agcacgacgc gtccgccggc cttgccattg
    21601 ccatccatgc cgagcgggta gtccgccatc tcgatgaccc acaggcggcc ctgctcatcc
    21661 caatcaaacg cgaccggatc gagcaccacc ggctccgccg cgacgagttc gatgcggtaa
    21721 ccgtcgcgca catgccactt cttcgcggat tcttcgggag aaagcggctg cgtggtggtt
    21781 ggaacagaat gcggggagtg atttcccaca gattcaggga ggcccgcaga ttttttcaga
    21841 agccaatctg tgggcttccc tgaatctgtg ggagaaccct tctgggcttc gatgagtttg
    21901 gatgaagttg tttgggtggt tcgcagagga tttgctgatg gctccaatcc gcttaccgcg
    21961 accttttcaa acgagccttc cagcggcgcg aagtcatcgc agcgtttgcc gaagaacaac
    22021 tccttcgcgc cgggcgcggt gacgggcagt tcggcgtcga tctccacctt gccattgagc
    22081 gtgaccttca cgcgcgagcc gaggcgctcc agcttcacgt cattccaagt gcccggctcg
    22141 atgaccgtcg tgccgcgcac gatttgcgag gcggcattgc cattgaaaac gaacaaccgg
    22201 cccggtgacg agtccttgta gttaccgccg atacccaggt gatcgcccgg cgcttgatga
    22261 tcgcccttcg gtccgcgcga gatgaggtag gcggtcacgg cgctgacgtt gttcgccttc
    22321 gtgttcttga agcgcatcga cgcggaccag ttttcatgcg ccttcagcaa ggccgagctc
    22381 tgcacctgcg cccgcttctt ccgctccgcc cgcgaaacgg cccgcttcgc attctcctcg
    22441 cccactttcg agatgttcca aagcttcgcg atgagcgaag gctcgatgac gcgatcccag
    22501 atcgtgatct catcgagccg gccttcgagt ccttgaccga aaagcacctc gttggccgct
    22561 ttcccggcct ttccggtcaa aacgggcttc tcggagccat cgagatgcac gcgcacgttt
    22621 tcgccatcgc ggatgagcac ggcgaagtgc cagtcatcgg caaagaaagt ggtttcggct
    22681 ttagccgaat ccgatccttc ggctgaagcc gaagccactt tatctccaaa ttcgagcctc
    22741 accgtgtgat ccaggaactg atgcgccttc aagctgacgc ccagcgcgtt gatcaactcg
    22801 cccgcgcgat cactcgcacc gctttcgtgg ccgagccaga accagagggc gatggaagcg
    22861 gctgatggtg ctggctcttt tggagaggct ctctccacag gcagattgct ccagtccgct
    22921 ttgatgtgac caccagcgac ctggacagcc ctgttgatgc tctgatcttt ggaaaaggga
    22981 gacagcttga gcgcggattc cacgccataa ccaagcccac tgccgacacc tggcagatac
    23041 atggcatgac ctccaaccaa tagcgcatcg tggacccatt cgtcagacct cattgagcga
    23101 gcgaatgaaa gcggtgaatg cagtttgggg ctgacgattt cgtcgcaact gtagtgggcc
    23161 gaaggttttg aaactttgat ttcgtgccca tagactccga cttctttctt ggcttcacgc
    23221 gcaacgcctg ttcctagaat tccgagggca taactgagtt gaggcagcat tttgctctcc
    23281 gcttctgcct cgagtccggc tgtgcgcgca ggccaagtcg tgtagccacc cagcttgtgc
    23341 tgctcatacg gcggtatgta gccttcagcc ccgttcgcca gctcaatgtt gaagtgagtc
    23401 ttgaatggcg accactctct cagcttcaat cccgtgattg catacacctc gttcggcagc
    23461 gtggcgatgc tcagatcacc gatgcggatg gcttggagct tcacgctggt cttttgccgc
    23521 tcatgcaaaa tcagcgcctc tctcgcatag acttcctctt tgttcttcgg caggtcgttt
    23581 tcgatcttcg cggcgatggg acgggcccat gcgaggcgtt tttcgtcggg gacgcggtag
    23641 ttgagctcca gcgtcttctc gaccatgccg agcggggcgt gatcgacata cttcacggtt
    23701 tgcagggcct tcagggcgct gtcggcgacc tcggaggcgt aggtgtcgat ggtgatcgtc
    23761 tttttctccg cgccgtagtc catccacatc tggtcgccgc tggtgccctg gctcatggcg
    23821 cagacgaagg ggccgttgcc atcgccctgc tggtccattt tcgcggcgag gtgcttgcag
    23881 aagaggccga agtagtcggc agagaccggc gaggtgccaa agtagtgctg cgagtagttc
    23941 gccagcacgc cgagcggttt gccatcgagc gtttgcagag cgatcacgct gagctgcggg
    24001 tccaccgggc cgctggggcc gacgacgtcc ttgctgaggt agccggggtg catgttcgcg
    24061 cggcccgtgg gctggccgaa gggatcgacg acttccttgc ccggcaggcg aatccagcgg
    24121 cggttgtgcg tgtgctccca gtcgtcgaac gagccccagc cgatgcgcgc cggttgcagc
    24181 gccgcattcg ccgccacgat ggcctcggcg atctttggcg tgaggaattt cgcatacacc
    24241 gtgtccttcc gcgtgccgag gcaacccatc gccgccggcg cggagtgcgt gtgcgtggcg
    24301 ctgaccatca tgcgatccac cgggatgccg cattgcttcg ccgcgatgcc tttggcctca
    24361 tcgatgagcg cctgctccat catgcaagtg tccacgatag cgaaggcgat cttcatcttc
    24421 ccatcatcga gcacgaaact gcgcacgaag agcttgtccg cgagcctttc gccccgtccc
    24481 tccagaaacc cgcccgcgat gatgcgcggg aactccgtgg gcgaaatgtc cactgtggcg
    24541 actccggcgc ggaatacagg ctgcgcactc aggctggcga ccgcgagcaa aaacgtgatg
    24601 atgggaatga cgcgcatgat gccggagggc acgcaagcgc gggggaagct ctttcagacg
    24661 ggcatccaat gaaggaaggc ggtctttcca gacgttggtc tccgcccccc caatgtcagt
    24721 ttttgccagt catcccatcc tccgtctctt tgcctcgccg cagcatttgt ggcggacggt
    24781 gtcgctgggc ttgaaaagca tctggctcaa caagctgcgc tcgtttttga ccgctctggg
    24841 cgtcatcttt ggcgtggcct cggtggtggc gatgctggcg atcggcgagg gcgcgagtca
    24901 tgaggcgcag gagcaaatcc gcaggctggg cagccagaac atcatcctgg aaagcgtgaa
    24961 gccgccggac gggcaggggt ccacgggcga gacgcgcagc atgatcatcg aatacgggct
    25021 cacgatgcgc gacatccagc agatccgcca gacggttccc ggagtgagcg tggtggtgcc
    25081 cagccgcatc ttgacggaga acatctggaa tctggaccgc agtgtggacg ggcagatcat
    25141 gggtgtgctg ccggtttatc cggaaatgcg caaccgccgc gtgttgcagg gccgcttttt
    25201 caccgacttg gagatgaacg aaaagctgcc cgtgtgcgtg ctcaatgaga cggcggcatc
    25261 caagctgttc ccgctttcaa cacccacggg caagtcggtg cgcgtgaagg gttactatta
    25321 caagatcgtg ggcatcctcg aggacgaagg ccagcgcgtc ggcacggatg gcgggccctc
    25381 cagcaacaac gcctccgccc aactcatgct gccttactcg acgctcatgg accagtatgg
    25441 cgagacgttt ttccgattcc gcagcggcag cttcgaggcg gaaaaagtgg agtttcatga
    25501 ggcggtggtg cgcgtggagg atgtgaacca ggtggagagc cgcgccaatg ccatccgcca
    25561 tctgctggcg aagaaccaca agaaagagga ctggcgcatc atcgtgccgg tggagctgct
    25621 gcggcaggcg gagcgcacga agcagatctt cagcatcgtg ctcggcagca tcgcggccat
    25681 ctcgctgctg gtgggcggca tcggcatcat gaacatcatg ctcgccagcg tcaccgagcg
    25741 cacgcgcgag attggcatcc gccgcgccat gggcgcgcgg caggcggaca ttgtgctgca
    25801 atttctcgtg gagaccgtgc tgctctccgg agcgggcggc atcatcggcg tggtgctcgg
    25861 cgtgtccatc ccggtggcgg tggagcactt cgctggcgtc accacggtgg tgaaaatatg
    25921 gtcacccgcg ctggcctttc tcatctccgt gctcaccggc atcggcttcg gcatctaccc
    25981 ggcgcggcgc gcggcgcgca tgaatccggt ggaggcgctg cggcatgagt gaggcttgat
    26041 aggcgagggg catccgatat ggggaacagg tgtctcgact gttcacacca tgcacaggca
    26101 ggatgcctgt gccccatcat gagcggtcgt ggttgagcat tgggtgcggc ttcactggaa
    26161 ccgggcagaa tgcccggttc acatgtgtca ggcggggacg cctgacctac tgccggcacg
    26221 gcccgtcttt ctctcaaact cccgccacag cctctcgatg gcctcctcgg ggtcttcgag
    26281 catcatgcct tccttgatct tgctggcgta gatgccgaag gagaaggggc tgacgacggg
    26341 cacatccacc agatgccagt ccagccgtgg ggcgcgctcc aggaactcga tggcgcgcgg
    26401 caaatccagg aaggtgtgtg tggactcgcg gtaggcctgc gcgagcatgg ggtgatccgg
    26461 ctcatgctcg gcgagcacgc ggaagaggat ctccgcgctc caccgcaggt gcttgaggcg
    26521 ctgctctttg ccggggcggt tgcgcgggac catgaggccg gtctgcgcca caccgcggaa
    26581 gttccacttc accagctcgc tctgctccag ggcggcgcgc atgtcgcgtt cgggatcacc
    26641 ggtggcgaag agctccctcc aggcatcgag ttcgaggtct tgaaacgact tcactgcgag
    26701 caggaagccg tagtcatcta tcgtgaccag ggtgttgccg ccgacggctt ccttcagccg
    26761 gtgggagatg atgcggctga gcgcgtcatt ggcgctgcgg ccgatgaggc tgtggaagaa
    26821 aaaatgcacg cgctcggtgt cggcaccttc tcggtcctcc acgaagcgct cgatgagaaa
    26881 ggtgtcgtct gtggggatgc gtgagacgcg cagctggttc cggcaatgac gcacgatggc
    26941 ctcggcattg gtctgggaga tctcgaatcg ctcgatgagc cagtcggtga tgccttccgg
    27001 ttcatccatg cgcgctgcca gctccgtgcg cagcagcgcc acctcgcgcg ccaggccgga
    27061 ggagagcggc attttgttcg cgttccaggc gggcacggtg ggcaggcggc ccacggcatc
    27121 ctccacgcgc gcctcagcca cactggtctc cattaagcgc acagtgcgcc cggccagcac
    27181 aaagatgtct cccagtttca gccgcttcaa aaagccctcc tcgatctgcc ccagccgccg
    27241 tttgccgagc aaaacgctca ccatgccctc ggtgtggatg acgcccacat tcgccagata
    27301 ctcgcgctcg accttttggt tcggcacgcc cagccgcccg tcccgccaga cgatcttgcc
    27361 gaagttctcg cggtattgct tttccaggga gcgcccgccg ccctccaggt aattcagcac
    27421 gcggtccagc tccgcctcag tgagactgcg gaagggaaat gccgcgcgca ccgtggccag
    27481 cacgctttcc cgtgtgtggc cgccctccat ggccatgccc acgagatgct gcgccagcac
    27541 atcgagcggg cactccagcg ggcgcaccgc gtccagccgt gtggcgcgtg tcatctcggc
    27601 gcagacgatg cattccatga ggtcgttcac attcgtggcg acgaggatgc cgtggctctc
    27661 cgtgtgaatg ctgtgcccgc tgcggccgat gcgctggagc gcgcggctga tgcccttcgg
    27721 cgtggagatc atcaccacgc agtccacgct gccgatgtcc acaccgagtt cgaggctggt
    27781 gctgcacacc acggcgcgca gctcgccgtt cttcagccgg tcctccacct ccaggcgcac
    27841 atcgcggtcc agggaggcgt ggtgcgcctc gatcttctcc gccagtttcg gcagcgcatt
    27901 tttgagacgg atggtgatgc tctccgtgct gctgcgcgtg ttgcaaaaga ggatcacatt
    27961 ccggctgcgc tcgacgatgc gcgcgatgtc ttccatgacc cgctgcgccg tgtagccggc
    28021 gctggggtaa ggatcacgcc gcagcgggga aagcacctcc acacgcctgc gccgctccat
    28081 gcgcgcctcg acgatctcac acgcccggcc cggcccgacc aggaactctg ccaacaaagg
    28141 cagcggcgcg gccgtggccg agaggccgat gcgggtagga caggcgtccc gcctgtcacg
    28201 ggtgaaccgg gcattctgcc cggtttcccc ggcaacgggc aggatgcccg ttgcacatga
    28261 gacaggcaga atgcctgacc tgcgcgccag c
//
LOCUS       DAOMYJ010000044        27579 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Prosthecobacter sp. isolate AMR_MDS_5629
            NODE_621_length_27579_cov_22.348678, whole genome shotgun sequence.
ACCESSION   DAOMYJ010000044 DAOMYJ010000000
VERSION     DAOMYJ010000044.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867850
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prosthecobacter sp. (wastewater metagenome)
  ORGANISM  Prosthecobacter sp.
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Verrucomicrobiaceae; Prosthecobacter.
REFERENCE   1  (bases 1 to 27579)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 27579)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.30
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:34:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,742
            CDSs (total)                      :: 4,684
            Genes (coding)                    :: 4,676
            CDSs (with protein)               :: 4,676
            Genes (RNA)                       :: 58
            tRNAs                             :: 55
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27579
                     /organism="Prosthecobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_621_length_27579_cov_22.348678"
                     /isolate="AMR_MDS_5629"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1965333"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            221..2809
                     /locus_tag="PLP58_06745"
     CDS             221..2809
                     /locus_tag="PLP58_06745"
                     /inference="COORDINATES: protein motif:HMM:NF017044.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HRJ08249.1"
                     /translation="MPAKRPAAPARRPVKKAAPASVEAPPMNAQARTLPNGLEVIVRE
                     DHEHPLVSVQLWVRAGSLHEEAWTGAGLAHCLEHMLFKGTWKRSAQEISQHIQQLGGY
                     VNAYTTFNRTVYYIDGLAAHLEGYLEILADMARHSKIDAAELEKEQDVIRREMAMCDD
                     DPGHALQDLVQATAFRAHPLRHPVIGHRAVFDQARQPDLAAFYQRHYAPNNCFVVIAG
                     AVSAREAFEIVERQLGSWERRPYEPAPLPAEPAQRGLREGRKNFATELTRVALGWQIP
                     GEGHEDKPALDVLAFLLGSGRSARLFQELREKRGIAHWVWAGAWGAQECGVFNAEVEC
                     DPEDETACRAAMLEVVEKMRAAGPDAAELDKAVRATTSGQLRALSTTKGQAAALGHGW
                     LHVGSLEHSRHYLEAVRALTPERIREMARRYLVPATLSVAVVGPGVETAASAGISATE
                     RAGVERVVLPNGLTLLVRENPRLPLVSIRAGFLASVPAETAGNAGVTMVSAALLLKGT
                     KKRAARQIASELENLGGSLHCSADAHRYTLGAEVMRGDEARAFDLLSDLIQNATLPAA
                     GLAEVKKRHLASIQEEMEDPLTVAMRRARRHIFEGLPFARTALGTEASVKALTVADCR
                     AMLRASFTGANGVISVDGDVSAAAIRQLVQKHLGRLPKGARRPVTLPEELAQLRAKAG
                     SWTQHMDKEQAVLVIGYRTTGLHDADTHALTLMDEACSDMGSRLFNRIREELGLAYYV
                     GAQSFAALGAGAFYFYVGTDPAKVSLARAEMLRLIADLAKNGLTAEEISRAKTAWKSS
                     WLRSQQGSGARADALGWNEINGLGHDHFQKLPGLIEAVTDKDIRRVAKTWFGKDRAFI
                     VEVLPK"
     gene            complement(2846..3787)
                     /gene="nadA"
                     /locus_tag="PLP58_06750"
     CDS             complement(2846..3787)
                     /gene="nadA"
                     /locus_tag="PLP58_06750"
                     /EC_number="2.5.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009963262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008987 - quinolinate synthetase A
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quinolinate synthase NadA"
                     /protein_id="HRJ08250.1"
                     /translation="MVAIATPTLVYEILRLKKERNAVILAHNYQTRDIQEIADFVGDS
                     LGLAYKAKETRADVIAFCGVHFMAETAKIVNPGKTVVLPDANAGCSLEQSCPGPQLEA
                     FLKANAAKNYYVIAYINCSAHVKALSDCICTSGNAVKIVQAAPPDRPILFVPDQNLGS
                     WVMEQTGRKMDLWKGACYVHVEFTRDSILAIKDKHPDAQVVAHPECTYAVRMLADEVC
                     STEKMVGYCRSHPAGAFIIVTESGMLHRLEREVPGKTFIPGPTGHCACADCRYMKLNT
                     LQKLHDALRDLEPRVELPEDIRARAEKPILRMLELSR"
     gene            3906..5705
                     /gene="ispG"
                     /locus_tag="PLP58_06755"
     CDS             3906..5705
                     /gene="ispG"
                     /locus_tag="PLP58_06755"
                     /EC_number="1.17.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018969131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0046429 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(E)-4-hydroxy-3-methylbut-2-enyl-diphosphate
                     synthase"
                     /protein_id="HRJ08251.1"
                     /translation="MNHASLRYCPDLYHYQRRETRVCMVGHVGIGGGNPVRVQSMLTS
                     DTRDTESCVNESLELASVGCEIVRVTAQTRVIAENLQHIRDGLRAAGCDVPLVADIHF
                     KPDAAMEAVKWVEKVRVNPGNYADKKKFAVKEYTDEQYAEEVAHMEAQFAPLVQECIR
                     LGRAMRIGTNHGSLSDRIMNRWGDTPLGMVESALEFARIARAQGFHNFLFSMKASNPK
                     VMIEAYRLLVAKLHELGGDWNYPLHLGVTEAGDGEDGRIKSAIGIGSLLADGIGDTIR
                     VSLTEDAIHEIPVARALVASVQAQARVDAASHIANPPPVSYDAFSYSRRASEKMQVQG
                     VDVGGGSTVAVFTSRMKWNALAHKLDKLGDYRPEIVVEDSGVTALDPRDEAALAAVNA
                     APEPRLVTIADGTPLQPVHAYRLLAARLDVRHPILLKDQFAVRPPDAEGDFTENLLTA
                     AMNLGSLLCDGIGDAVLVQGESAPGQSLRVSYNILQAAGVRIFKTDYVACPSCGRTLF
                     NLQNTTQKIRAATGHLKGVRIAVMGCIVNGPGEMADADFGYVGGAPGKINLYVGKTAV
                     KFNIPEEEAVERLVDLIKEHDRWVEGGAIVSEA"
     gene            complement(5752..6597)
                     /locus_tag="PLP58_06760"
     CDS             complement(5752..6597)
                     /locus_tag="PLP58_06760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ08252.1"
                     /translation="MNESSQSRRQWLAALLPAALGACVQPAAAPSRAGRAARAYAEYQ
                     RTFAVSGAHFRYHPYGGQGRAWTMQMERLWQGLRQDARVGYIVKNLRSGAVLAEQQAD
                     RFFFGSSMPKPAISAMLLEKRQGSLSHEEFMHVVRVCDKSENSSWMALHARLYPQDEA
                     NFLWKYRLPQASVLGNQLTPRFIAEFYERCVNHRLDHGTWLLLEAMRRDQYGFGRLYL
                     PAHIAYAGGKTGNYGEWDHESLFFEDRGTPYAIVVFTRGHVSPGKMNLPLGALMGGLY
                     RAHVG"
     gene            6719..7489
                     /locus_tag="PLP58_06765"
     CDS             6719..7489
                     /locus_tag="PLP58_06765"
                     /EC_number="3.2.2.4"
                     /inference="COORDINATES: protein motif:HMM:NF005500.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008714 - AMP nucleosidase activity
                     [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA];
                     GO_process: GO:0044209 - AMP salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AMP nucleosidase"
                     /protein_id="HRJ08253.1"
                     /translation="MTSRTAIVENWLPRYTGTPLEEFGGYILLTNFNQYVKLFAQWHR
                     VKVRGKDKPMPNATAGDISIINFGMGSATAATVMDLLSAIKPKAVLFLGKCGGLKHRG
                     SIGDFILPIAAIRGDGTSNDYFPPEVPALPAFALQKAISTTIRDHGRDYWTGTVYTTN
                     RRVWEHDEKFKTYLKELRAIAIDMETATIFMTGFFNEIPTGALLLVSDQPMIPEGIKT
                     AESDQKVDADHVADHLRIGMDSLKQLINKGLTVKHLKF"
     gene            7541..8041
                     /locus_tag="PLP58_06770"
     CDS             7541..8041
                     /locus_tag="PLP58_06770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ08254.1"
                     /translation="MSSLGVRPHFTQILDLDVEAARERIATRAAAHEGRCEVKNFPGF
                     VCLRVPVVERRYWSPRLNLSLDPHEGGRTRAEGVYGPNANMWSSFLYAWLLVGSVGLF
                     SGIFGACQYTLGMWPWGLWIFAVMAVLALTLYALARFGRRMGAEQTRQLHEIYQECVG
                     KPVEVE"
     gene            complement(8316..9200)
                     /locus_tag="PLP58_06775"
     CDS             complement(8316..9200)
                     /locus_tag="PLP58_06775"
                     /inference="COORDINATES: protein motif:HMM:NF022750.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3108 domain-containing protein"
                     /protein_id="HRJ08255.1"
                     /translation="MKTRRRFLLACGLCAPWMRARTLWSQSVMKAATPPGWAGLVSRA
                     APGGFAALPPCRVVYDLSWNNLISAGSAEVTMRQAGAFQVAKAQAGTTGFARRLWRYD
                     CVMSSVIERGSLRARYLEHSETDDRETISYQAAFSSSQVTTESVLTPKKGAEKKSTAI
                     CPYGPVDDLQSAILYVRSHPLKAGDAITRVVQPFDRPYLVTFTVAGREKRKVRGTAHD
                     TIKLDVKIRKIDRKTLALGSFKKMRKATIWVSDDAWRLPVEAHADIFVGFISAAITKR
                     EALTGTDAITSLPKSMTR"
     gene            complement(9254..9850)
                     /locus_tag="PLP58_06780"
     CDS             complement(9254..9850)
                     /locus_tag="PLP58_06780"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF025217.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HRJ08256.1"
                     /translation="MKDLPPNPIQQDMAKLKSLLHDSLSPHLLPGQKDMRILNLACGR
                     CDEAETLIQVGNDLTNGGAVEMVGADIRIREIRQARETHGHLPTEFLIEDATKISQHK
                     ELGDDFNMVFLRHQNFWHGQELWKKIFDQGIAKLRPDGMLVITSYFDVEHRLALRALE
                     ALGMEVVSNKRNKSSRALKDAPGKSVDRWVAVLRRRRA"
     gene            complement(9909..11372)
                     /locus_tag="PLP58_06785"
     CDS             complement(9909..11372)
                     /locus_tag="PLP58_06785"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase"
                     /protein_id="HRJ08257.1"
                     /translation="MKTLITATLLALACSLPAAGKPNFILINIDDQGYADIGPYGSDN
                     ATPNLDRMAREGMKLTSHYAAPVCSPSRAMMMTGCYPKRVLPTPHVLFPAGAIGLNPD
                     ETTLAEVLKSRGYATACIGKWHLGDQPEFLPTAQGFDTYYGIPYSNDMGTAAEGSKSN
                     PGQPMPDAKAAAKKKAQPASDEAGIRGNAQPPLPLVENLDAIERIKQDGQHSITRRYT
                     EKALDFIRQSKDGPFFLYLPHTAVHFPLYPDKQHMGKSPNGLVGDWAQELDSSTGQIL
                     DLLRELGLAENTLVIYTSDNGGALNHSSKNTPLRGGKGQTYEGGIRVATLAWWPGKIH
                     AGSSTDAITAHFDFLPTFAALAGASLPERKLDGKDISPLLLGKLGAKSRDTFYYYRGH
                     KLEAVRFGMWKLHLDKGELYSLREDIGESNDIAASHPDQVAQLRELAQKMAADLGLDG
                     PDAPGVRPLGRVADPLPFIAHDGKVRPGADGKYKHLD"
     gene            11525..12937
                     /locus_tag="PLP58_06790"
     CDS             11525..12937
                     /locus_tag="PLP58_06790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ08258.1"
                     /translation="MPLIPMKASSWMPVFLLVSTLPSAGADALEDLLMPRVEASKLSD
                     ERANAAWRMVVEAFHRNDMEKAVELGKAFMSSDLKTSAYQLLGVKVMLGLAGGADSGS
                     MFVSRADNEEKKKLEEERASITERYQELATIYRDADARINLLTSNRTRPVQQGSTNHL
                     ECLRCARLMEDAKSGMDALKRPVEENKKKMARLEEKAKSGMKPQTLELLDMLIAADEI
                     EAAFAIANTYIRTAGNDLDIARKQQDVVRLREVGDKAAKVMALLQEEIKDLVAGKKYW
                     EAWAKTGQFLDRVGQLNHDEELLKMVRSRTALDPLGVRKKMIEGEESHRLIKQHAAVD
                     FTRAWQDFVVFESAYPDHPGRKELELHIATAKARSLDAILSQLDFDFQDVQRRFNPAK
                     LSAYATETASGGGAAAASSSQKLLDLGLAPADVRLVHSKLQGMNAAVGMVEKIGVPQD
                     RMVRLTEIKTTLAALATLLQ"
     gene            13027..13914
                     /locus_tag="PLP58_06795"
     CDS             13027..13914
                     /locus_tag="PLP58_06795"
                     /inference="COORDINATES: protein motif:HMM:NF024816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HRJ08259.1"
                     /translation="MTASLSSFSHLLDNHAADLLAQGRLEEALGAAQTALAGAREAAA
                     EDPGAQPGLFSGLCLLADIQRELGDAAGAEASYGEALEIASTCAEVPRASVAGARTQL
                     ATLLDFSQREADAAPLYEQAIEDYEALDPPEFEASAQLRNNLAMIYKGLGRFALAEQH
                     YLKALELLEARRGRDSEDVGSIYNNLGSLYYTAGFAGQAREMFTEALEIRARLLGPDH
                     PDVAQSHSNLATACHELQDDPAALGHFEQGLLILEKHAKDDPAAYEAVALDYLALLES
                     LDEGKKAAALKKRMEKVLA"
     gene            complement(13920..15902)
                     /gene="waaF"
                     /locus_tag="PLP58_06800"
     CDS             complement(13920..15902)
                     /gene="waaF"
                     /locus_tag="PLP58_06800"
                     /inference="COORDINATES: protein motif:HMM:TIGR02195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008920 - lipopolysaccharide
                     heptosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide heptosyltransferase II"
                     /protein_id="HRJ08260.1"
                     /translation="MGKSASKALDTLQLAGHWLVYALFRCFEGVLRLLPMEWVWHLGR
                     CIGLAGYFLLGKYRLLALRNLRLAFGAEHDEAWRRRVARAHFGSLVANILCGFKLPTL
                     EGAEVMRRVKIEGMEPAREAAEAGRPVVYLLCHMSCWELLTQLPRVFEYNKNPATIYQ
                     PLRNPFLDALVTRRRRKLGYVLFDRQAGFNGPIKHLRESGCLGVLVDQHAGNQGLWCP
                     FFNRLASTTPVAALMAQRCRGLMMPVGIHDDGPGHWRITGLPPVDTTTAGLSIEGLTC
                     WMNAAVEKLVRRQPHNWFWVHNRWKTPKPRFLLSREKRGVALPEDLTAERLQKFEMLL
                     RSPNWLGDACMALPAAHALKQGRPDLRLTVFGPEKLRGLWEAQPWVDAYIGKQGGEGP
                     LSVARKLRASGVCFDAALLLTNSTRSTLEFRLAGIPRLAGFRGSLRALFLNQIIKEPK
                     ISGPPWHHARRYLHLAGRCGASTEGWEKDLPVNQAPAGVNRVGVCAGAEYGPAKRWPL
                     ENFAAVIASLGARMPELEWIFFGAPGEAAMGEDLSKKAARARHSNLVGKTSLAGLITE
                     LKTCRLLLTNDTGTMHLAAALGVPTVSIFGSTEPVLTGPLGAGHTIIRHHVPCSPCFK
                     RECPFGHYECMTGVTPDRVSAAVEAALHAAPSPAKA"
     gene            16000..16374
                     /gene="ndhC"
                     /locus_tag="PLP58_06805"
     CDS             16000..16374
                     /gene="ndhC"
                     /locus_tag="PLP58_06805"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012420587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit A"
                     /protein_id="HRJ08261.1"
                     /translation="MLENYLPVLLQILIAAGFAGGTLAASVLAGKAARRNAVKDSAYE
                     CGMLPLGGAQPRFSVKFYIVAMLFVLFDIEVVFLYPWAVIFRDQIAVFGLGIAWAALG
                     FIGILGVAFVYAWRKGALDWKH"
     gene            16387..16704
                     /locus_tag="PLP58_06810"
     CDS             16387..16704
                     /locus_tag="PLP58_06810"
                     /inference="COORDINATES: protein motif:HMM:NF019491.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dabb family protein"
                     /protein_id="HRJ08262.1"
                     /translation="MIAFISLYKLRPEVTPEKLEDMMSRTRVSLLRIPEVLAVKTGKR
                     VNPSDSFEWFVYLEVESMDKLAICQDDPHYIKYREEVLKPNVAEQEAEAFEMEPRKDV
                     KFS"
     gene            complement(16720..17508)
                     /locus_tag="PLP58_06815"
     CDS             complement(16720..17508)
                     /locus_tag="PLP58_06815"
                     /inference="COORDINATES: protein motif:HMM:NF015297.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase/citrate lyase family protein"
                     /protein_id="HRJ08263.1"
                     /translation="MPVRPSQVMRLVHENQPAHGVAVRLMDTIVPEFVSMIGFDAIWL
                     DLEHHHLSVEKAAEMIRAARAGGPADVVARPGKGEFTRMARLLEAGAKGIMYPRCESA
                     AEAAEVVRWAKFPPLGERGLDGAAADNPYRLTPVADYLRQANQETFLIAQIESPGALD
                     QAAAIAAVPGIDFLMLGPGDFSASIGAPGQMNHPGVVAAHERVRAAAHGAGKHWAVTC
                     FDMAHAGECLSKGARLLFHGSDWRLVNNGLQQLVQQARGLGAGG"
     gene            17600..18595
                     /locus_tag="PLP58_06820"
     CDS             17600..18595
                     /locus_tag="PLP58_06820"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HRJ08264.1"
                     /translation="MKYLPRDLSTLGQTAKSALLVLCGLAGFARGHFAGQGCILTFHG
                     LRRADSPPGVLDSGLHLPVDLFRQICARLAMRYHVMPLSEMARKVSQGQSLPEHAVAL
                     NFDDGYASNYELAWPVLKEFGLPATIFLTTGFVDEGRPLWFHEADLAMTCQGASTRRL
                     VRALRRLKTLPEEQMRAEVAGLVSEAGGLGELPEVLRPLTWQQAREMQAGGLVEFGGH
                     THTHPILSRCTVERQREEIVKCRDRMEAELGRRPRLFGIPNGYPADFDKTTLAELAAA
                     GFEHAFTMMPGRVKASCHPLKIPRYGAPVSVWETEATASGAFELVRVWKAKGGPG"
     gene            18781..19950
                     /locus_tag="PLP58_06825"
     CDS             18781..19950
                     /locus_tag="PLP58_06825"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HRJ08265.1"
                     /translation="MPAGETTSQAAGAGRRTVLHFTPSIGGGGAEAMLVNLVLAMDGA
                     RWRRVVVVVNGSAWPETISRLRDAGVEVHDLGAPAFLCKAALSGLTRLLRQTRPDVVQ
                     TWMHHADFIGGWCARLAGVRHVVWGVHCREIHRNPGDSDFKMRVFRWLLGASSHVVPA
                     RVVSCSAAALEEHMAWGYPRASLEWVPNGILTRRFGPSEEIRAETRRRMLLPADAPVV
                     GFVGRFHEMKDLPTWLRAAALLQTRRPETHFWLCGLGEQELDDAARAALSIMPRRAQV
                     HFTPFSHEPEKVYPALDVFSLSSRTEACPMTVIEALSCGTPCVTTDVGDCARLIENAG
                     RVVPAGDAEALARAWDEVLAAPRDARALHEHAAERFDIATAARAYERIYEEVIAG"
     gene            19947..21662
                     /locus_tag="PLP58_06830"
     CDS             19947..21662
                     /locus_tag="PLP58_06830"
                     /inference="COORDINATES: protein motif:HMM:NF024716.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4091 domain-containing protein"
                     /protein_id="HRJ08266.1"
                     /translation="MMRLIPCLALLFGQGHAALSLRLVDGMTRVPRSGAMAEQTPDLR
                     AARGEWEPVQVVLSGAAAELKGVTVEVTALTAGEKSVIAEPEVLRAHYVRVSKPTPMS
                     PMPPSEHADALVPQDFPWQELPQDTENVNQPFWVDLHVPYTAQPGAHSGGIRVRKDGA
                     EVLTHPLSLTVLDFDMPTVPRLRSSIMTVWRRIAEAHGFDRQQEPPAPELAALLEQYH
                     DLLARHRLSMDTIHPTFPDHRTGRLDETAVEMALRKHLLHRHASTISLPIWSQWPFAD
                     PLGADREAAQLYAATWMKVVKKLRCEARGYVIHSDLDEPNSADDYAMVRRWGDFFNEA
                     EHKHGVRLPLLITEQPTPDDPWWGRFDGSVDIWVPHISSVWQDMEAPDGKRDIERRVK
                     AGDEVWCYAALVQMPDAWERAHGKPEVLRESNPPVWCLDFPAMNHRMPAWVMPLHGIT
                     GFCYWDTLHAEPGVDVWAEAGNFLHPEAGIYNGDGSYIYPATRERHGRHMPVASVRLK
                     WLREMTEDYDYIMLARDLGLRNEAQLLADTFARGFGDWEDRMDKLFEARRKLADLIER
                     KGGRS"
     gene            21659..22948
                     /locus_tag="PLP58_06835"
     CDS             21659..22948
                     /locus_tag="PLP58_06835"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HRJ08267.1"
                     /translation="MKVLVLTADANTLVYHRGDLIRAFARAGCQVVTSAAEDYPHVRA
                     YVEAAGGRHEPIRMVRSRVNPAKDWITWLDMFRLFRKERPDALFAYTIKSVVYGCVVA
                     RLAGVPRVYALLPGLGFTFVKPETLKQALVQWISKALHRMALRRADVIFMQNRDDVRL
                     FEELRLLPAGVPVHVTAGSGVNLEEYPHRPMENDAALAEGRVRFVLVSRLLISKGVRV
                     FAEAARRVRARFPQAEFHLVGPFDPNPNRVGEAEVSQWVAEGTLTHHGMVRDVAALLE
                     TMHIFCLPTWYREGVPHATLEALATGRPVITTDSVGARECVRGAGPDCWGENGCLVPV
                     RDVDAVVRAMERLLENPAHIPRMGRASRRLAAEVFDVNLVNDIILRAMGLPASSVGVQ
                     LSGCETLPDRLKPELQHQETTATQTPATTPCRVAAGA"
     gene            22973..25378
                     /locus_tag="PLP58_06840"
     CDS             22973..25378
                     /locus_tag="PLP58_06840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ08268.1"
                     /translation="MALHLPLDTLRQPPGHPGQTGRSLVPMAPAGLPAAYLPQVDASP
                     YLATPPPQFTESFINLAELVGYMRRFGWLGFLAGLALAALAFYALGMGTPIYEAEAKL
                     RLRLQDTNVFNFNEMGRQSVTELSAPQLINNHLTEIKSRKYLEYFHDRFPEADRARFI
                     EVESTQVGRKDQLLKLLGLYKPGIPSPPRETFMDGMSARVRVEPQKDSHILRVLVRHP
                     DPGMAARIANAYATEYMGFFGQQEGSQMDSARTYLEEKAAELRQRLQESEAKLAEYRK
                     SENLLQDSEVKDVAGERVRLFTNAITDAQVRLFKAQSDLRNIQSAQQAGRDLMEVRVV
                     ADNAEVAYNRKELDAKIAQRKALEPMCGRRHPMMIALANEIETLKMALDRSVDAVVAQ
                     AQSEVTNMERQVADYQNEMNTSRSQSLEQSGKNIQQNLLRDQVAMDRELYQKIVLRLN
                     QATVTGQFTDSGALALSDVAAPPNKPVKPNKPVAALASLFLFGVCFIGLPVGWGLFND
                     HVLGTLRRGGSPSPHIPSARQVPHMPLQPTGPLPQQAVHAALPQQAYAPAPVPVMQPP
                     AQQQRAGETLMIPAVGQTPVLARLPMLGSGRPEHILGQILKPEPQGAAGALQQLTSTL
                     EMHALRRGGLGGVILITSAEAGEGKTLSAAALAAAFCHQGRSVFLLECNPVAPALHEW
                     FPHSARHSSWAHDMEALRYGNTHLFLLPAHDLPAYATTELLEGYRSWIDRARQQVDWI
                     ILDAGPVLRNFADVAPLAPLATDVLLVNNPRVTNAGKLRAALHLLQPMMASSAFRGLV
                     MHG"
     gene            25477..27195
                     /locus_tag="PLP58_06845"
     CDS             25477..27195
                     /locus_tag="PLP58_06845"
                     /inference="COORDINATES: protein motif:HMM:NF014325.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-binding protein"
                     /protein_id="HRJ08269.1"
                     /translation="MKALLAVLNPVMENRTSRTNLRALANLILVLAGLVAVFSLLFHV
                     LMVREGQEHSWITGLYWTLTVMTTLGFGDITFQGDLGRLFSVVVLCSGVVFMLVLLPF
                     TFIEFFYAPWMRAQAAAKAPRELPASTSGHVILTDHGPLATTLIPMLEKYGHPYVVLC
                     PTLQEALDLDERGVRVAVGDLDDPETYRRMRVEQAAMLVATRADVINTNATFTARELA
                     ERVPIVASASSDSARDVLELAGATLVLRLEEMMGSALARRVSIRDSDAHVIGNLDGLL
                     IAEAAAHGTPLAGQTLAQSGLRTRTGVSVIGVWDHGRLAVAEADMMIRTDTIFVLAGT
                     REQLDRYNAAFPNDTTSKPRVLIIGGGRVGRATSRVLRDLGIQTTIIEKIPERVAGHP
                     EAVIGDATQMDVLKKAAAREATTLIITTHDDDTNVALTIFFRRLRSNWQILARSTLDR
                     NLKTLLRAGADLVLSYSSMGANTVFNLLRGGDHLLLAEGVNVFPSPIPESVAGRRLGE
                     LEIRSQTGCTVIAVESRGKREINPGPELLMPAGGRMFLIGTLEAEEKFLELHKPAQPL
                     KRGRRH"
     gene            27250..27528
                     /locus_tag="PLP58_06850"
     CDS             27250..27528
                     /locus_tag="PLP58_06850"
                     /inference="COORDINATES: protein motif:HMM:NF012759.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantetheine-binding protein"
                     /protein_id="HRJ08270.1"
                     /translation="MSEAENATRISVPALIRLMREEGVIEPENPLTGQCDLFACGMDS
                     LAMMRLMILIERDFGIRIPAEQVTRDRFASADALAEWLGSLHRPPFPV"
BASE COUNT         4719 a         8992 c         9135 g         4733 t
ORIGIN      
        1 acagccgccc gcccggccca cgctggtgcg cgacggcaag gcgcggctgc tggtgggaaa
       61 gacgccctat gaggtgcctc tggaccgcct ttcggatgag gaccgcgact tcatccaggc
      121 tctggaaaaa gccgtcgccg cctcgtccga ggcgcagggc aagtgacgcg cgcctcgccg
      181 cttgccgcag ggcgcatccc gctccatgca tccgcccctc atgccagcca aacgccccgc
      241 cgcacccgcc cgccgtcctg tgaaaaaagc cgcgcccgct tctgtcgaag cgccgccgat
      301 gaacgcgcag gcccgcactt tgccgaacgg cttggaggtc atcgtgcgcg aggaccatga
      361 gcatccgctt gtgagcgtgc agctctgggt cagggccggc agcctgcatg aggaggcgtg
      421 gactggcgcg ggtctggcgc actgcctgga gcacatgctt ttcaagggca cctggaagcg
      481 ctccgcccag gagatctccc agcacatcca gcagctcggt ggttatgtga acgcctacac
      541 caccttcaac cgcacggtct attacattga cggcctggcc gcgcaccttg agggttacct
      601 ggagattttg gccgacatgg cgcgccactc gaagattgac gccgccgagc tggaaaaaga
      661 gcaggatgtc atccgccgcg agatggccat gtgcgatgac gaccccggcc acgcgctgca
      721 ggacctcgtg caggccaccg ccttccgcgc gcatccgctg cggcacccgg tcatcggcca
      781 ccgcgccgtc ttcgaccagg cgcgccagcc ggacctggcc gccttttacc agcgccatta
      841 tgcgccgaac aactgcttcg tcgtcatcgc cggagccgtg tccgcgcggg aggccttcga
      901 gatcgtcgag cgccagctcg gctcctggga gcgccgccct tacgagcccg cgcccctgcc
      961 tgcggagccc gcccagcgcg gcctgcgcga ggggcggaaa aacttcgcca ccgagctcac
     1021 gcgcgtggcc ctgggctggc aaatccccgg cgaggggcat gaggacaagc ccgcgctcga
     1081 tgtgctggct tttctgctcg gctccggccg cagcgcgcgg cttttccagg agctgcgcga
     1141 gaagcgcggc atcgcccact gggtgtgggc cggcgcgtgg ggcgcgcagg aatgcggcgt
     1201 cttcaatgcc gaggtggagt gcgacccgga ggacgagacc gcctgccgcg ccgccatgct
     1261 ggaggtggtg gagaaaatgc gcgccgccgg gcctgacgcc gccgaacttg acaaggccgt
     1321 gcgcgccacc accagcggcc agctccgcgc actgagcacc acgaagggcc aggccgccgc
     1381 gctcggccac ggctggctgc atgtgggcag cctggagcac tcgcgccatt acctggaggc
     1441 cgtgcgcgct ctcaccccgg agcgcatccg cgagatggcg cggcgttacc tggtgccggc
     1501 gacgctgagc gtggctgtcg tcggccccgg ggtcgagacc gccgccagcg ccggcatctc
     1561 cgccacggag cgcgccggcg tggagcgcgt cgttttgccg aacggcctca ccctgctcgt
     1621 gcgggagaat ccgcgcctgc cgctcgtctc catccgcgcc ggcttccttg ccagcgtgcc
     1681 cgccgagacc gccggcaatg ccggcgtcac catggtatcc gccgcgctgc ttttgaaggg
     1741 cacgaaaaag cgcgccgcca ggcagatcgc ctccgagctg gagaacctcg gcggcagcct
     1801 gcactgctcc gccgacgcgc accgttacac cctgggtgcc gaggtcatgc gcggggacga
     1861 ggcgcgcgca tttgatctgt tgtccgacct gatccaaaac gccaccctgc ccgccgccgg
     1921 gctcgccgag gtgaagaagc gccacctggc cagcatccag gaggagatgg aggacccgct
     1981 caccgtggcc atgcgccgcg cgcggcggca catcttcgag gggctgccct tcgcgcgcac
     2041 cgcgctgggc accgaggcaa gcgtgaaggc gctcaccgtg gcggattgcc gcgccatgct
     2101 gcgcgcctca ttcaccggcg ccaacggcgt catcagcgtg gatggcgatg tctcagccgc
     2161 cgccatccgc cagcttgtcc agaaacacct gggcaggctg cccaagggcg cgcgccgccc
     2221 ggtgacgctg ccggaggagc tggcgcagct ccgcgccaaa gccgggagct ggacgcagca
     2281 catggacaag gagcaggccg tgctcgtcat cggttaccgc actaccggcc tgcatgacgc
     2341 ggacacccac gcgctcactt tgatggacga ggcttgcagc gacatgggca gccgcctttt
     2401 caaccgcatc cgcgaggagc tcggcctggc ctattatgtg ggcgcgcaaa gcttcgccgc
     2461 gctcggcgcg ggcgcgtttt atttctacgt cggcaccgat ccggcaaagg tctccctggc
     2521 ccgggccgag atgctgcgcc tcatcgccga cctggcaaaa aacggcctca ctgcggagga
     2581 aatcagccgt gccaagaccg cgtggaaatc ctcctggctg cgctcccagc agggcagcgg
     2641 cgcgcgcgcc gacgcgctcg gctggaacga gatcaacggc ctcggccacg accacttcca
     2701 gaagctgccc ggcctcatcg aagccgtcac cgacaaggac atccgccgcg tggcgaagac
     2761 ctggttcggc aaagaccgcg ccttcatcgt cgaggtgctg ccgaagtagg ccggaagtcc
     2821 ccgcctaaag tggctcggct tttcctcacc ggctcagctc cagcatgcgc aggatgggct
     2881 tttcggcgcg ggcgcggatg tcctcgggca gctcgacgcg gggttccagg tcgcgcagcg
     2941 cgtcgtggag cttttgcagg gtgttcagct tcatgtagcg gcagtcggcg caggcgcagt
     3001 ggccggtggg gccggggatg aaggttttcc ccggcacctc gcgctccagg cggtggagca
     3061 tgccgctctc ggtgacgatg atgaaggcgc cggcggggtg gctgcggcag tagccgacca
     3121 ttttctcggt gctgcacacc tcgtcggcga gcatgcgcac ggcgtaggtg cattcggggt
     3181 gggccacgac ctgggcgtcc gggtgcttgt ctttgatggc caggatgctg tcgcgcgtga
     3241 actcgacgtg gacgtagcaa gcgcccttcc agaggtccat tttgcgcccg gtctgctcca
     3301 tgacccagga gccgaggttt tgatccggca cgaagaggat gggccggtcc ggcggggcgg
     3361 cctggacgat tttgacggcg ttgccgctgg tgcagatgca gtcactgagg gctttgacgt
     3421 gggcggagca gttgatgtag gcgatgacgt aatagttttt ggcggcgttg gctttgagga
     3481 aggcctcaag ctgcgggccg gggcagcttt gctccaggga gcagccggcg ttggcatcgg
     3541 gcaggacgac ggtcttgccg gggttcacga tcttggcggt ctcggccatg aaatggacgc
     3601 cgcagaaggc gatgacatcg gcgcgggtct ccttcgcctt gtaggcgagg ccgagggagt
     3661 cgccgacaaa atcggcgatc tcctggatgt cgcgcgtctg gtagttgtgc gcgaggatga
     3721 cggcgttgcg ctcctttttc aggcgcagga tctcgtagac cagggtgggg gtggcgatgg
     3781 cgaccatgcg ggatgtttaa agcggcgcgg cgcgcgggca agggcggctt caagcgcgcg
     3841 gcgggccgcg cttcagaagc cgctggcatg agcgcgcggc cagtgcatga tggcgctccc
     3901 gcctcatgaa ccacgccagc cttcgctact gcccggacct ctaccattac cagcgccgcg
     3961 agacgcgcgt gtgcatggtg ggccatgtgg gcatcggcgg cggcaacccc gtccgcgtgc
     4021 agtccatgct caccagcgac acgcgcgaca ccgaatcctg cgtcaatgag tcgctggagc
     4081 tcgccagtgt cggttgcgag atcgtgcgtg tcacggcgca gacgcgcgtc attgccgaaa
     4141 atctccagca catccgcgac ggcctgcgcg ccgctggttg tgacgtgccc ctggtggccg
     4201 acatccactt caagccggat gcagccatgg aggccgtgaa gtgggtggaa aaagtgcgcg
     4261 tcaaccccgg caactacgcc gacaaaaaga aattcgccgt caaggaatac accgacgagc
     4321 aatacgccga ggaggtggcg cacatggagg cgcagttcgc gccgctcgtg caggagtgca
     4381 tccgcctggg ccgcgccatg cgcatcggca ccaaccacgg ctccctgagc gaccgcatca
     4441 tgaaccgctg gggtgacaca cccctgggca tggtggagag cgcgctggag ttcgcccgca
     4501 tcgccagggc gcagggtttt cacaactttc tgttcagcat gaaggccagc aacccgaagg
     4561 tcatgatcga ggcctaccgc ctgctggtgg ccaagctcca tgaactcggc ggtgactgga
     4621 actaccccct gcacctcggc gtgaccgaag cgggtgacgg cgaggacggc cgcatcaaaa
     4681 gcgccatcgg catcggctcc ctgctcgccg acggcatcgg cgacaccatt cgcgtctccc
     4741 tcaccgagga cgccatccac gagatccccg tcgcgcgcgc tctcgtcgcc tccgtgcagg
     4801 cgcaggcgcg cgtggatgcg gcttcacaca tcgctaatcc tccgccagtg agctatgacg
     4861 cattcagcta cagccggcgc gcttcggaga aaatgcaggt ccagggagtg gacgtgggcg
     4921 gcggctccac ggtggccgtt ttcacctcgc gcatgaagtg gaatgcgctc gctcacaagc
     4981 tcgacaagct cggcgattac cgccctgaga tcgtcgtgga agacagcggc gtcaccgcgc
     5041 tcgacccgcg tgatgaggcc gcgctcgccg ccgtcaatgc cgcgccagag ccaaggctcg
     5101 tcaccatcgc cgacggcacg cccttgcagc ccgtccacgc ctaccgcctt ctggccgcgc
     5161 ggttggatgt ccggcatccc attttgctca aggaccagtt tgcggtccgc ccgccggatg
     5221 ctgagggaga cttcaccgag aacctgctca ccgccgccat gaatctcggc tccctgctct
     5281 gtgacggaat cggcgacgct gtgcttgtgc aaggcgagtc cgcgcccggc cagtctttgc
     5341 gcgttagcta caacatcctg caagccgccg gcgtgcgcat tttcaaaacc gactatgtgg
     5401 cctgcccgag ctgcggacgc acgctcttca acctgcaaaa caccacgcaa aaaatccgcg
     5461 ccgccaccgg ccacctcaaa ggcgtgcgca tcgccgtcat gggctgcatt gtcaacggcc
     5521 ccggtgagat ggccgacgcc gacttcggct acgtgggcgg agcgccgggc aagatcaacc
     5581 tctacgtcgg caagacggcg gtgaagttca acatcccgga ggaggaggcc gtggagcggc
     5641 tggtggacct catcaaagaa cacgaccgct gggtggaagg tggggcaatc gtctcagagg
     5701 catgaatgga accgggcaga atgcccggtt cacatgggac aggcaggatg cctatcccac
     5761 atgcgcccga tacagtccgc ccatcagcgc gcccagcggc agattcatct tccccggcga
     5821 gacatgcccg cgcgtgaaga ccacgatggc atacggcgtg ccgcggtcct cgaagaacag
     5881 ggactcgtga tcccactcgc catagttgcc cgtcttgccc ccggcgtaag cgatgtgcgc
     5941 gggcagataa agtcggccaa agccatattg atcccgccgc atcgcctcca gcagcagcca
     6001 ggtgccgtgg tccaggcggt ggttgacgca gcgctcgtaa aattcggcga tgaagcgcgg
     6061 cgtgagctga ttgcccagca cgctggcttg aggcagccgg tatttccaaa gaaaattcgc
     6121 ctcatcctgc gggtacagcc gcgcgtgcaa agccatccag gaggagtttt cgcttttgtc
     6181 gcagaccctc accacatgca tgaactcctc gtgactgaga ctgccctgcc gcttctccaa
     6241 cagcatcgct gagatggccg gcttcggcat cgagctgcca aagaaaaaac ggtccgcctg
     6301 ctgctccgcc agcaccgccc cggagcgcag atttttcacg atgtaaccca cgcgcgcatc
     6361 ctgcctcagt ccctgccaga gccgctccat ttgcatggtc catgcgcgtc cctgcccgcc
     6421 ataaggatgg tagcggaaat gcgcgcctga gacggcaaag gtgcgttgat attccgcata
     6481 cgcccgcgcc gcacgccccg ccctcgaagg tgccgcagca ggctgcacac aggcgccgag
     6541 agcggcgggc agcagtgccg ccagccactg ccggcggctc tgggatgatt cgttcatgtt
     6601 accctgggac atgcggtgaa agtgagctgc gcatgtcttt atatgccagc cccggcggcg
     6661 gaaagacaaa accacggccg tgccttgcag gagtgtcgca aaagcgcgaa actccgccat
     6721 gacctcgcgc accgccatcg tggaaaactg gctgccacgc tacaccggca cccctctgga
     6781 agagttcggc ggctacatcc tgctgacgaa cttcaaccaa tatgtgaaac tcttcgccca
     6841 gtggcaccgc gtcaaagtgc gcggcaagga caagcccatg cccaacgcca ccgctggcga
     6901 catcagcatc atcaacttcg gcatgggcag cgccaccgcc gccaccgtca tggacctgct
     6961 cagcgccatc aaaccgaaag ccgtgctctt ccttggcaag tgcggcggcc tcaagcatcg
     7021 cggcagcatt ggcgacttca tcctccccat cgccgccatc cggggtgatg gcaccagcaa
     7081 cgactacttt ccacccgagg tgccggcgct gcccgccttc gccctgcaaa aagccatctc
     7141 caccaccatc cgcgaccatg gccgcgacta ctggaccggc acagtctata ccaccaaccg
     7201 ccgcgtctgg gagcacgacg agaaattcaa aacctacctc aaggagctgc gcgccattgc
     7261 catagacatg gagaccgcca ccatcttcat gaccggcttc ttcaacgaaa tccccaccgg
     7321 cgccctcctc ctcgtctccg accagcccat gatccccgaa ggcatcaaaa ccgccgagag
     7381 cgatcaaaaa gtggatgccg accatgtcgc cgaccacctg cgcatcggca tggactcttt
     7441 gaagcaactc atcaacaaag gtctgactgt gaagcatttg aagttttgac ccgctcgcca
     7501 tctgtgagag gtccgtttac gatgcgacgt actcgtaggc atgagcagtc tcggcgtccg
     7561 cccgcacttc acgcagatcc tcgaccttga tgtggaggcg gcgcgggagc ggattgccac
     7621 gcgggcggcg gcgcatgagg ggcgctgcga ggtgaaaaat ttccccggtt ttgtctgcct
     7681 gcgcgtgccg gtggtggagc gccgttactg gtcgcccagg ctgaatttga gtctggatcc
     7741 gcatgagggc gggcgcacgc gggcggaggg cgtttatggg ccgaatgcga acatgtggtc
     7801 ctcctttctc tatgcgtggc tgctggtggg cagcgtgggg cttttctccg gcatcttcgg
     7861 tgcctgccaa tacacgctgg gcatgtggcc gtgggggttg tggatcttcg cggtcatggc
     7921 cgtgctggcg ctcactttgt atgcgctggc gcgctttggc cgcaggatgg gcgcggagca
     7981 gacacggcag ttgcacgaga tttatcagga gtgcgtgggc aagcccgtgg aggtggagtg
     8041 actgctctgt aggcgcattc gccagaatgc gggggctgtg cgtgtatgcg ggagcttttc
     8101 ccgtgctttg gcaagcgcgc ctacagggga agatacgact ctcagccgtg acgatgcagt
     8161 tgaaccacga atggacacga atcgacacga atccatgcga taaagagagg gattggcgtc
     8221 atcaccaaag agtgatctcc cggcgcttca gatcaccggg catgaggcct ccaaatttcg
     8281 tgtccattcg tggtttccca tgacatgaac acggctcagc gtgtcatgct tttgggcagg
     8341 ctggtgatgg catccgtgcc ggtgagtgcc tcgcgcttgg ttatcgcggc ggagatgaag
     8401 ccgacgaaga tgtccgcgtg ggcctcgacc ggcagccgcc aggcatcgtc gctgacccag
     8461 atggtggctt tgcgcatctt tttgaagctg cccagcgcga gcgtttttct gtctatcttc
     8521 cggatcttca catcgagctt gatggtgtca tgcgctgtgc cccgcacctt gcgtttctca
     8581 cgccctgcca cggtgaaggt gacaagatac gggcggtcaa aaggctgcac cacgcgcgtg
     8641 atcgcatccc ccgctttcag cgggtggctg cggacgtaaa gaatggcgga ttgcaggtca
     8701 tccacaggac cgtaggggca gatggcggtg ctttttttct ccgcgccttt tttcggggtg
     8761 aggacgcttt ccgtcgtcac ctgggaggag gaaaaggcgg cctggtaaga gatcgtctcc
     8821 cggtcatccg tctcggagtg ctccagatat cgcgcccgca aacttccgcg ctcgatcacg
     8881 gatgacatga cgcaatcata acgccagagc cgccgcgcaa aacccgtggt gcccgcttgc
     8941 gccttcgcca cttgaaaagc acccgcctgc cgcatggtca cctctgcgga ccctgcggag
     9001 atgaggttgt tccagctcag atcataaacc acgcggcagg gaggcagcgc cgcaaaaccg
     9061 ccaggcgcgg cacggctcac cagaccggcc caacccggag gtgtggccgc cttcatgaca
     9121 ctctggctcc acaaagtccg cgcccgcatc cagggggcgc acaatccgca ggcgagcaaa
     9181 aaacgacgac gtgttttcat ggcggcaggc tctgactctg gtggcggctt tttcttcaat
     9241 cttgccggaa atgtcacgcc cgccgccggc gcagcacggc cacccagcgg tccacggact
     9301 tgcctggcgc gtccttcaat gcgcggctgg acttgttgcg cttgttgctg acgacctcca
     9361 tgcccagcgc ctccagcgcg cgcagcgcca gccggtgctc cacgtcgaag taactggtga
     9421 tgacgagcat tccgtcaggc cgaagcttgg cgatgccttg atcaaaaatc ttcttccaca
     9481 gctcctgccc gtgccagaaa ttctgatggc gcaggaagac catgttgaag tcatcgccca
     9541 gctccttgtg ctggctgatc ttggtggcgt cctcgatcaa aaactccgtc ggcagatgcc
     9601 cgtgtgtctc gcgcgcctgg cggatctcgc ggatgcggat gtccgcgccc accatctcga
     9661 ccgcgccacc gttcgtgagg tcattgccga cctggatcaa agtctccgcc tcatcacaac
     9721 gtccacaggc caggttcaaa atgcgcatgt ctttctgtcc cggcagcaaa tgcggcgaga
     9781 gcgagtcatg gagaagcgat ttgagctttg ccatgtcctg ctggatggga tttggaggga
     9841 ggtctttcat gagggtcttg cgcgacaatc cttgaaaatg ccgcattcgc ctgcctcagc
     9901 tcggggactt aatcaaggtg cttgtacttg ccgtccgcgc cggggcgaac ctttccgtca
     9961 tgcgcgatga agggcagggg atccgccacg cggccgagcg ggcgcacacc gggcgcatcg
    10021 gggccgtcga ggccgaggtc ggcagccatc ttttgagcga gttcgcgcag ctgtgccacc
    10081 tgatcggggt ggctggcggc gatgtcattc gattcgccga tgtcctcgcg caggctgtaa
    10141 agctcgcctt tgtcgagatg cagcttccac attccgaagc ggacggcctc cagcttgtgc
    10201 ccgcggtagt aataaaaagt gtcgcggctt ttggcgccga gttttccaag caggaggggc
    10261 gagatgtctt tgccgtcgag tttgcgctcc ggcaatgacg cgcccgcgag cgcggcgaaa
    10321 gtgggaagga aatcgaagtg cgcggtgatg gcgtcggtgg aactgccggc gtgaattttt
    10381 cccggccacc aggcgagcgt ggccacgcgg atgccgcctt cataggtctg gcccttgccg
    10441 ccgcgcagag gtgtgttttt ggagctgtga ttgagagcgc cgccgttgtc gctggtgtag
    10501 atgacgagcg tgttctcagc gagtccaagc tcgcgaagca agtcgaggat ttgaccggtg
    10561 ctggagtcga gttcctgcgc ccagtcacca acaaggccgt tgggggactt gcccatgtgc
    10621 tgcttgtccg gataaagggg gaagtggacg gcggtgtgcg gcaggtagag aaaaaacggc
    10681 ccgtccttgc tttggcggat gaagtcgagc gccttctccg tgtaccggcg ggtgatgcta
    10741 tgctgcccgt cctgcttgat tcgctctatg gcgtcgaggt tctccacgag cggcaggggg
    10801 ggctgggcat taccgcggat gccggcttca tcgctggcgg gctgggcctt cttcttggcg
    10861 gcggcctttg catcgggcat gggctggccg gggttgcttt tggagccttc ggcggcggtg
    10921 cccatgtcat tggagtaagg gatgccgtaa taagtgtcga agccctgcgc cgtgggcagg
    10981 aactcaggct ggtctccaag gtgccatttg ccgatgcagg ctgtggcata gccccgggat
    11041 ttgagcacct cggcgagggt ggtttcatcg ggattcaaac cgatggcccc ggctggaaag
    11101 aggacatgcg gcgtgggcag gacgcgtttc gggtagcagc cggtcatcat catggcgcgc
    11161 gagggcgagc agacaggcgc ggcgtaatgg ctggtgagtt tcatgccctc gcgcgccatg
    11221 cggtcgagat tcggcgtggc attgtcggag ccgtaggggc cgatgtccgc gtaaccctgg
    11281 tcgtcaatat tgatgaggat gaagttcggc ttgccggctg ccgggaggga gcaggcgagc
    11341 gcgaggagcg tggcggtgat gagtgtcttc atggcttttg gagtgcctgg aaacggagat
    11401 gaagggcagt tctcatccgg gaattggcat cgggaggcaa ccgccggtag cgtgaaattt
    11461 tttttgcgcc aggcgcattt tgcgtttgcc agccggcggc gtttcaccgc gtgatctcgg
    11521 ccgaatgcca ctcatcccta tgaaagccag ctcatggatg cctgttttcc tccttgtctc
    11581 gacgcttcca tccgccgggg ctgatgcctt ggaggatttg ctgatgccgc gcgtcgaggc
    11641 atcaaagctt tccgacgagc gggcaaacgc ggcctggagg atggtggtcg aggcgtttca
    11701 tcgcaacgac atggagaagg ccgtcgaact gggcaaggcg ttcatgagca gcgacctcaa
    11761 gacctccgcc taccagttgc tcggggtcaa ggtcatgcta ggcctggcag gcggcgctga
    11821 cagcggcagc atgttcgtca gccgcgcgga caatgaggaa aagaagaagc tcgaggagga
    11881 gcgcgcctcc atcacggagc gctaccagga actggcgacc atttaccggg atgccgacgc
    11941 gcgcatcaac ctgctgacca gcaaccgcac ccggcccgtg cagcaggggt ccaccaatca
    12001 tctcgaatgc ctgcgctgcg cccgtctcat ggaggacgca aagtccggca tggacgcgct
    12061 gaagaggccg gtcgaggaaa acaaaaagaa gatggccagg ctggaggaga aagccaaatc
    12121 tggaatgaag ccgcagactt tggaactgct cgacatgtta atcgcagcgg atgagatcga
    12181 ggccgccttc gccatcgcca acacctacat ccgcaccgcc ggcaatgacc tcgacatcgc
    12241 cagaaagcag caggatgtcg tgcgtctgcg cgaagtgggt gacaaggccg cgaaagtcat
    12301 ggccctgctc caggaggaga tcaaagacct cgtggccggc aaaaagtact gggaggcttg
    12361 ggcgaaaacc ggccagttcc ttgaccgcgt agggcagctc aaccacgacg aggagctgct
    12421 caaaatggtg cgctccagga cggcgctgga cccgctcggc gtgcgcaaaa agatgatcga
    12481 gggcgaggaa agccaccggc tgatcaagca gcacgcggcg gtggatttca cgcgcgcctg
    12541 gcaggatttc gtggtgtttg agagcgctta ccccgaccat cccgggcgca aagagctgga
    12601 actgcatatc gccaccgcca aggcccggtc actggacgcc atcctcagcc agttggactt
    12661 cgattttcag gatgtgcagc gccggttcaa ccccgcgaag ctgagcgcct acgccactga
    12721 aaccgcgtca ggcggcgggg cggcggctgc ctccagttcg cagaagctgc tcgatctcgg
    12781 cctggctccg gcggatgtgc gactggtgca ttccaagctc cagggcatga acgccgcagt
    12841 gggcatggtg gagaaaatcg gcgtgccgca ggaccgcatg gtcaggctca cggagatcaa
    12901 gaccaccctg gcggcgctcg ccactctgct gcagtgagag aagaggcccg ctgagttttc
    12961 gcttgctgaa aggagcggga tgccgcgatg gtaccgcgtt cccacgtcaa ccaccccctt
    13021 gactccgtga ccgcgagcct ttcgagcttt tcccatcttc tggacaacca tgccgccgac
    13081 cttctagccc agggcaggct ggaggaggct ctgggcgcgg ctcaaacggc cctggccggc
    13141 gcgcgcgagg cggctgcgga ggatccgggc gcgcagcccg gtctcttttc aggcctgtgc
    13201 ctgctggcgg acatccagcg cgagctgggt gacgcggcgg gggcggaggc cagctatggc
    13261 gaggctttgg agatcgcctc cacctgcgcg gaagtgccgc gcgcgagtgt cgccggcgct
    13321 cgcacgcaac tggccacgct gctggacttc agccagcgcg aggcggacgc ggctccgctt
    13381 tatgagcaag ccatcgagga ttatgaggcc ctggatccgc cggagttcga ggcgtctgcg
    13441 cagctccgca acaatctggc catgatttac aaggggctgg ggaggttcgc gctggccgag
    13501 cagcattatt tgaaggcgct ggagctcctg gaggcaagac gcgggcgcga ctccgaggac
    13561 gtgggctcca tttataacaa tctgggcagt ctttactaca cggcgggttt tgccggccag
    13621 gcgcgggaga tgttcaccga ggcactggaa atccgcgcac gcctgctcgg ccccgaccac
    13681 cctgatgtgg cgcagtccca cagcaacctc gccacggcct gccatgagct gcaggatgac
    13741 cccgccgccc tggggcactt cgagcaaggg ctgctcatcc tcgaaaagca cgcgaaggat
    13801 gaccctgccg cctacgaggc cgtggcgctg gattatctgg ctctgctgga aagtctggat
    13861 gaggggaaaa aggcggccgc gttgaaaaaa cgcatggaga aggtgctggc ctgaggcggt
    13921 caggctttcg ctggagaggg cgcggcgtga agcgcggctt cgacagccgc cgacacgcgg
    13981 tcgggcgtga ctccggtcat gcattcatag tgaccgaagg gacactcgcg tttgaagcag
    14041 gggctgcacg gcacatgatg acggatgatg gtgtggcccg cgccgagcgg accggtgagc
    14101 accggctcgg tgctgccgaa aatgctcacc gtgggcacgc cgagggcggc tgccaggtgc
    14161 atggtgccgg tgtcattggt cagcagcagg cggcaggttt tgagttcggt gatgagtcct
    14221 gcgaggctgg tcttgccgac gaggttcgaa tgccgcgcgc gtgccgcttt ttttgacagg
    14281 tcctccccca tggccgcctc gcccggcgct ccgaagaaga tccattcaag ctccggcatc
    14341 cgggcgccga ggctggcgat gactgccgcg aagttctcca gcggccagcg cttggcgggt
    14401 ccatactcag cgccggcgca gacgcccacg cggttcactc cggccggcgc ctggttcacg
    14461 ggaaggtctt tctcccagcc ctccgtgctc gcgccgcagc gtccggcgag gtggaggtag
    14521 cgccgcgcat gatgccaggg cggaccggag atcttcggct ccttgatgat ctggttcaga
    14581 aacagagcgc gcagcgatcc tctgaagcct gccagtctcg ggatgcccgc cagccggaac
    14641 tccagcgtgc tgcgggtcga gttcgtcagc agcagcgccg cgtcaaaaca cacgcctgac
    14701 gcgcgcagct tccgcgccac ggagagcggc ccctcaccgc cctgcttgcc gatgtaggcg
    14761 tccacccagg gctgcgcctc ccacaggccg cgcagttttt ccgggccgaa aacggtgagc
    14821 cgcaggtccg ggcggccttg cttcagcgcg tgggcggcgg gcagggccat gcaggcgtcg
    14881 ccgagccagt ttggggagcg gagcagcatc tcgaattttt gcagccgctc ggcggtcaag
    14941 tcctcgggca gagccacgcc gcgtttttcc cggcttaaca agaagcgcgg tttcggtgtc
    15001 ttccagcggt tgtggaccca gaaccagttg tgcggctggc ggcgcaccag tttctccacc
    15061 gccgcgttca tccagcaggt caggccctcg atggagaggc cggcagtggt cgtgtccacc
    15121 ggcggcagtc ccgtgatgcg ccagtgtccc gggccgtcgt catggatgcc caccggcatc
    15181 atcagtccgc ggcagcgctg cgccatgagc gccgccacgg gcgtggtgga ggccagcctg
    15241 ttgaaaaagg ggcaccacag gccctggttg cccgcgtgct ggtccaccag gacgcccagg
    15301 cagccggact cgcgcaggtg tttgataggc ccgttgaagc ccgcctgccg gtcaaaaagc
    15361 acatagccca gcttgcgccg gcggcgcgtg accagcgcgt cgagaaaggg attgcgcaga
    15421 ggctggtaga tggtggcggg attcttgttg tattcgaaga cgcgcgggag ctgggtcagc
    15481 agctcccagc agctcatgtg gcagagcaga tagaccaccg ggcgcccggc ctcggcggct
    15541 tcgcgcgcgg gttccatgcc ctcgattttc acccgccgca tcacctccgc gccttcgagc
    15601 gtgggcagct tgaagccgca aaggatgttc gccaccaggg agccgaaatg cgcgcgcgcc
    15661 acccgccgcc gccaggcctc gtcatgctcg gcgccgaagg ccaggcgcag gttgcgcaag
    15721 gccagcaggc ggtatttgcc cagcaggaaa tagcccgcca gcccgatgca gcgccccaga
    15781 tgccacaccc attccatcgg cagcagccgc agcactccct cgaagcaccg gaacagcgcg
    15841 tacaccagcc agtgcccggc cagttgcagt gtgtcgagag ctttggatgc ggatttcccc
    15901 atcgtttgac attgacccaa gcgccgcggg ctgcaaccac gggaggcgtt tgtttcggcg
    15961 tcttgacgcg gggcgttgag cgtttagcat cccgcgccca tgctcgaaaa ctatctcccc
    16021 gttcttctgc aaatcctcat cgccgccggt ttcgccggag gcacgctggc ggcctcggtg
    16081 ctggcgggga aggccgccag gcgcaatgcg gtgaaggaca gcgcctacga gtgcgggatg
    16141 ctgccgctgg gcggcgcgca gccgcgcttc agtgtgaagt tttacatcgt ggcgatgctc
    16201 tttgtgctct tcgacatcga ggtcgttttc ctgtatccct gggcggtgat tttccgcgac
    16261 cagatcgcgg tcttcggtct tggcatcgcc tgggcggcgc tgggtttcat cggcatcctc
    16321 ggcgtggcct ttgtctatgc ctggcgcaaa ggggcgctgg attggaagca ttgaccggcg
    16381 gcacgcatga tcgccttcat cagtctctac aagctcaggc cggaggtcac gccggaaaag
    16441 ctggaggaca tgatgtcccg cacccgggtt tccctgctgc gcatcccgga ggtgctggcg
    16501 gtgaagacgg ggaagcgagt caacccgagc gactcctttg aatggttcgt ctatctggag
    16561 gtcgagagca tggacaagct ggccatctgc caggacgatc cgcattacat caaataccgc
    16621 gaggaggtgc tcaagccgaa cgtggccgag caggaggccg aggctttcga gatggagccg
    16681 cgcaaggatg tgaagttctc gtgacggacg gcgccgctct caaccgcccg cgccgagtcc
    16741 gcgcgcttgc tggacgagct gctgcaggcc gttgttgacg agacgccagt ccgaaccgtg
    16801 gaacaggagc ctggcgccct tggagaggca ttccccagca tgggccatgt cgaaacaggt
    16861 gacggcccag tgcttgccag cgccatgcgc ggcggcgcgg actcgctcat gggcggccac
    16921 cacgccggga tggttcatct gtccgggcgc gccgatgctg gcactgaagt cgccggggcc
    16981 gagcatgaga aaatcaatgc ccggcacagc ggcgatggcg gcggcctggt cgagagcgcc
    17041 ggggctttcg atttgggcga tgaggaaggt ctcctggttc gcctggcgga ggtagtcggc
    17101 cacgggggtg aggcggtagg ggttgtcggc ggcggcgccg tccagaccgc gctcgccgag
    17161 cgggggaaat ttggcccagc gcaccacttc tgcggcctcg gcggcggatt cgcagcgtgg
    17221 gtacatgatg cctttggctc ccgcctccag cagacgggcc atgcgggtga actcgccttt
    17281 gcctgggcgg gccaccacgt ctgccgggcc gccggcacgc gcggcacgga tcatttcagc
    17341 agctttttcc acgctgaggt gatggtgctc cagatcgagc cagatggcgt caaagccgat
    17401 catggagacg aattccggca caatcgtgtc catcagcctg accgcgacac cgtgggcggg
    17461 ctggttttca tggacgagac gcatgacttg gctggggcgg acgggcatga cggcgttgag
    17521 tcgttcgaaa tggcactttg gcaagagaag gctttgcaat gggggcctga ttatggcaat
    17581 ttattgaaat tataaagatg tgaagtatct gccccgcgat ctctcaaccc tcggccagac
    17641 tgcaaaatcc gcccttctcg tactatgcgg gctggcgggc tttgcgcgcg gacattttgc
    17701 cgggcagggc tgcatcctta cttttcacgg cctgcggcgc gcggactcgc ctccgggggt
    17761 gctggacagc gggttgcatc tgccggtgga tctttttcgt caaatatgcg cccgtctcgc
    17821 catgcggtac catgtgatgc ctctctcgga gatggcccgg aaggtgagcc agggacagtc
    17881 gctgccggag cacgcggtgg cgctcaattt cgacgacggc tatgcctcga actacgagct
    17941 ggcctggccg gtgctcaagg agttcggcct gccggcgacg atttttttga ccacgggatt
    18001 cgtggatgag gggaggccgc tttggttcca cgaggcggat ctggcgatga catgccaggg
    18061 agccagcacg cggcggctgg tgcgcgcgct gaggcggctg aagacgctgc cggaggagca
    18121 gatgcgcgcc gaggtcgcgg ggctggtgag tgaggcgggc gggctggggg agctgccgga
    18181 ggtgctgcgc ccgctgacgt ggcagcaggc gcgggagatg caggcggggg gcttggtgga
    18241 gttcggcggg cacacgcaca cgcaccccat cctctcccgc tgcacggtgg agcggcagcg
    18301 ggaggagatc gtgaaatgcc gggaccggat ggaggcggag ctggggcggc ggccgcggct
    18361 gttcggcatc ccgaacggct acccggcgga ctttgacaag accacgctcg cggagctggc
    18421 ggcggcgggc tttgagcatg cctttaccat gatgccgggc cgggtgaagg cgtcgtgcca
    18481 cccgctgaag atcccgcgct atggcgcgcc ggtgtcggtg tgggagacgg aggcgacggc
    18541 gtcgggcgcg ttcgagctgg tgcgcgtctg gaaggcgaaa ggagggccgg gatgaatcac
    18601 atgataagac cggtgatcgg gcgcggagcg gtcgtaggcc gggtgtgtcc ggcgggaaac
    18661 gcatccgcac ccgtcgcgca actcccgccg ccggaccgcc cggcttgcgg cagcggggag
    18721 ttccggcttg agcgcgcgtc cacaaagccg ggcgggctgt cgccacaccc ggcctgcgac
    18781 atgccggccg gggagacgac ctcgcaggcg gcgggagcgg gccggcggac ggtgctgcat
    18841 ttcacgccgt ccatcggcgg cggcggcgcg gaggcgatgc tggtgaacct ggtgctggcc
    18901 atggatgggg cgcgctggcg gcgcgtggtg gtggtggtga atggctcggc ctggccggag
    18961 acaatctccc ggctgcgtga cgcgggtgtg gaggtgcatg acctgggcgc gccggctttt
    19021 ctgtgcaagg ccgcgctttc cggcctgacg cgcctgctgc ggcagacgcg gccagatgtg
    19081 gtgcagacct ggatgcacca cgcggacttc atcggcggct ggtgcgcgcg gctggcgggg
    19141 gtgcggcatg tggtgtgggg ggttcactgc cgggagatcc accggaatcc gggggactcg
    19201 gacttcaaga tgcgcgtctt ccgctggctg ctgggcgcgt cctcgcatgt ggtgccggcg
    19261 cgggtcgtgt cctgctcggc ggcggcgctg gaggagcaca tggcgtgggg ctatccgcgc
    19321 gcgagcttgg agtgggtgcc aaacggcatc ctgacgcggc gcttcggccc ctcggaggaa
    19381 atccgtgcgg agacgcggag gcgcatgctg ctgccggcgg acgcgccggt ggtaggcttc
    19441 gtgggccgct tccatgagat gaaggacctg cccacctggc tgcgcgccgc cgctctgctc
    19501 cagacccgcc ggccggagac gcacttctgg ctctgcggtc tgggcgagca ggagctggac
    19561 gatgccgcgc gcgccgcgct ctcgatcatg ccccggcgcg cgcaggttca cttcacgccc
    19621 ttctcgcacg agccggagaa ggtgtatccg gcgctggatg tcttttccct ctcctcgcgc
    19681 acggaggcat gcccgatgac ggtcattgag gcgctctcct gcggcacgcc ctgcgtgacc
    19741 acggacgtgg gcgactgcgc gcggctgatc gaaaatgcgg gccgcgtggt gccggcggga
    19801 gatgccgagg cgctcgcccg cgcctgggat gaggtgctgg ccgcgccgcg tgacgcgcgg
    19861 gcgctgcacg agcacgctgc ggagcgcttc gacatcgcca ccgcggcgcg ggcttacgag
    19921 cggatttatg aggaggtgat cgcgggatga tgaggctcat tccatgcctt gccctgcttt
    19981 tcggccaggg gcacgctgcc ctctccctcc gcctcgtgga tggcatgacg cgcgtgccac
    20041 gcagcggggc gatggcggag cagacgccgg atttgcgcgc cgcgcgcggc gagtgggagc
    20101 cggtgcaggt ggtgctcagc ggcgcggcgg cggagttgaa gggtgtcact gtggaggtga
    20161 cggcgctcac ggcgggggag aagtcggtca ttgccgaacc ggaagtgctg cgcgcgcatt
    20221 atgtgcgggt ctccaagccc acgccgatgt cccccatgcc gcccagcgag cacgcggacg
    20281 cgctggtgcc gcaggacttc ccctggcagg agctgcccca ggacacggaa aatgtgaacc
    20341 agcccttctg ggtggacctg catgtaccct acaccgcgca gccgggagcg cacagcggcg
    20401 gcatccgcgt gcggaaggac ggcgcggagg tcctcaccca cccgctcagc ctcacggtgc
    20461 tggactttga catgcccacc gtgccgcgtc tgcgcagctc catcatgacc gtgtggcggc
    20521 gcatcgccga ggcgcacggc tttgaccgcc agcaggagcc gcccgcgccg gagctggccg
    20581 cgctgctgga gcagtatcac gacctgctgg cgcggcaccg gcttagcatg gacaccatcc
    20641 accccacctt ccccgaccac cgcaccggca ggctcgacga gaccgcggtg gagatggccc
    20701 tgcgcaagca tctgctgcac cgccatgcca gcaccatcag cctgcccatc tggtcgcagt
    20761 ggcccttcgc cgacccgctc ggcgcggacc gggaggcggc gcagctttac gcggccacct
    20821 ggatgaaggt ggtgaaaaag ctgcgctgcg aagcacgcgg ctacgtcatc cacagcgatc
    20881 tcgacgagcc gaacagcgct gatgattacg ccatggtgcg ccgctggggg gacttcttca
    20941 acgaggcgga gcacaagcac ggcgtgcgcc tgcccctgct catcaccgag cagcccacgc
    21001 cggacgatcc ctggtggggc aggttcgacg gcagcgtgga catctgggtg ccgcacatca
    21061 gctccgtgtg gcaggacatg gaggcgcctg acgggaagcg cgacatcgag cgccgcgtca
    21121 aggccggcga cgaggtctgg tgctacgcgg cgctcgtgca gatgccggac gcgtgggagc
    21181 gggcgcacgg caaaccggag gtgctgcgcg agtcgaaccc gcctgtgtgg tgcctcgact
    21241 tccccgcaat gaaccaccgc atgcccgcct gggtcatgcc gctgcatggc atcaccggct
    21301 tttgctactg ggacacgctg cacgccgagc cgggcgtgga tgtgtgggct gaagcgggga
    21361 atttcctcca ccccgaggcc ggcatctaca atggcgacgg cagctacatc taccccgcca
    21421 cgcgggagcg ccacggacgc cacatgcccg tggccagcgt gcgcctcaag tggctgcgcg
    21481 agatgaccga ggactacgat tacatcatgc tggcgcgcga tctcggcctg cgcaatgagg
    21541 cccaactgct cgccgacacc ttcgcgcgcg gctttggcga ctgggaggac cgcatggaca
    21601 agctcttcga ggcgaggcgc aagctggccg acctcatcga gaggaaagga ggccgctcat
    21661 gaaagtgctc gtcctcaccg ccgatgccaa cacgctcgtc tatcaccgcg gcgacctcat
    21721 ccgcgccttc gcccgcgcgg gctgccaggt cgtcacctcc gccgccgagg actacccgca
    21781 tgtgcgcgcc tatgtcgagg cggcgggcgg acgccacgag cccatccgca tggtgcgcag
    21841 ccgggtgaac ccggccaaag actggatcac ctggctggac atgttccgcc ttttcagaaa
    21901 ggagcggccc gacgccctct tcgcctacac catcaaatcc gtcgtctatg gctgcgtggt
    21961 ggcgcggctg gcgggcgtgc cgcgtgtgta tgcgctgctg cccgggctgg gcttcacctt
    22021 tgtcaaaccg gagaccctga agcaggcctt ggtgcagtgg atttcgaagg cgctgcaccg
    22081 catggccctg cgccgcgcgg atgtcatctt catgcagaac cgggacgatg tgcgcctttt
    22141 tgaggagctg cgtctgctgc ccgccggcgt gcccgtgcat gtcaccgcag gctccggcgt
    22201 gaacctggag gagtatccac accggcccat ggaaaacgat gccgcgttgg ctgaagggcg
    22261 cgtccgcttc gtgctcgtca gccggctgct catcagcaaa ggcgtgcgcg tctttgccga
    22321 ggcggcgcgg agggtgcggg cgcgcttccc gcaggccgag tttcatcttg tgggtccctt
    22381 tgacccgaac cccaaccgcg tcggggaggc ggaggtctcg caatgggtgg ccgagggcac
    22441 gctcacccat cacggcatgg tgcgggatgt ggccgcgctg ctggagacga tgcacatttt
    22501 ctgcctgccc acctggtatc gcgagggcgt gccgcacgcc acgctggagg ccctggccac
    22561 cggaaggccc gtcatcacca ctgactccgt cggcgcgcgc gagtgcgtgc gcggcgcggg
    22621 gcctgactgc tggggggaaa acggctgcct cgtgcccgtg cgggacgtgg atgccgtggt
    22681 ccgcgccatg gagcgcctgc tggaaaatcc ggcccacatt ccgcgcatgg gccgcgcaag
    22741 ccgccgcctt gccgccgaag tcttcgatgt caatctcgtc aacgacatca tcctgcgcgc
    22801 catgggcctg cccgcctctt ctgttggagt tcagctttca ggctgcgaga cgctaccaga
    22861 caggctaaag cctgaactcc aacaccaaga gaccaccgcc acgcaaactc ccgccaccac
    22921 gccttgccga gtcgcggccg gtgcatgatg ttaggcacct ttcctcctgt tcatggccct
    22981 ccacctcccg ctcgacaccc tgcgccagcc gcccggccac cccggccaga cggggcgcag
    23041 tctcgtgccc atggccccgg ccggcctgcc cgccgcctac ctgccgcagg tggatgcgag
    23101 tccctatctg gccacgccgc cgccgcagtt caccgagtcg ttcatcaatc tggcggagct
    23161 cgtcggttac atgcgccgct tcggctggct gggtttcctg gcgggtctgg cgctcgccgc
    23221 cctggccttt tacgccctgg gcatgggcac gcccatttat gaggccgagg ccaagctgcg
    23281 tctccgcctc caggacacga acgtctttaa ctttaacgag atgggccgcc agagcgtcac
    23341 cgaactcagc gccccccagc tcatcaacaa ccacctcacc gagatcaagt cccgcaagta
    23401 tctcgaatac ttccacgacc gcttccccga ggccgaccgc gcgcgattta ttgaagtcga
    23461 gtccacccag gtcggccgca aagaccagct tctcaaactc ctcggccttt acaaacccgg
    23521 cattccatcc ccgccgcgcg agaccttcat ggacggcatg agcgcccgtg tgcgcgtgga
    23581 gccgcagaaa gactcccaca tcctgcgcgt gctcgtgcgc catccagacc caggcatggc
    23641 cgcgcggatc gccaatgctt atgccacgga atacatgggc ttcttcggcc agcaggaagg
    23701 ctcccagatg gacagtgctc gcacctacct ggaggaaaaa gccgccgagc tgcgccagcg
    23761 actccaggag agcgaggcaa agctggccga ataccgcaag tcagaaaacc tgctccagga
    23821 cagcgaggtc aaagacgtgg ccggcgagcg cgtgcgcctc ttcaccaacg ccatcaccga
    23881 cgcccaggtg cgcctgttca aggcccagag cgacctgcgc aacatccagt ccgcccagca
    23941 agccgggcgc gacctcatgg aggtgcgcgt ggtggccgac aacgccgagg tggcctacaa
    24001 ccgcaaggaa ctcgacgcca agatcgccca gcgcaaagcc ctcgagccca tgtgcggtcg
    24061 ccgccacccc atgatgatcg cgctggccaa cgagatcgag accctcaaaa tggccctcga
    24121 ccgctccgtg gacgccgtcg tcgcccaggc ccagtccgag gtcacgaaca tggagcgcca
    24181 ggtggccgac tatcagaacg agatgaacac ctcccgcagc cagtccctgg agcaaagcgg
    24241 caagaacatc cagcagaacc tcctgcgcga ccaggtggcc atggatcgcg agctttatca
    24301 aaaaatcgtc ctgcgcctca atcaggccac cgtcaccggc cagttcaccg acagcggcgc
    24361 tctggccctc tccgacgtgg ccgcgccgcc gaacaaaccc gtgaagccga acaagcccgt
    24421 ggccgccctg gccagccttt tccttttcgg cgtctgcttc atcggcctgc ccgttggctg
    24481 ggggcttttc aatgaccatg ttctgggaac gctgcgccgg ggcggttcgc cttcgccgca
    24541 catcccctcc gcccggcagg tgccgcacat gccgctccag cccaccgggc cgctgccgca
    24601 gcaggccgtt catgccgcct tgccgcagca ggcttatgcg cccgcgccag ttccggtgat
    24661 gcagccgccc gcacagcagc agcgcgcggg ggagacattg atgattcccg ctgtgggcca
    24721 gacccccgtg ctggcccgcc tgcccatgct cggcagcgga cggccggagc acatcctcgg
    24781 ccagatttta aagcccgaac cccagggcgc ggcgggggct ctccagcagc tcaccagcac
    24841 gctcgaaatg cacgccctgc gccgcggcgg tctcggcggt gtcattctca tcaccagcgc
    24901 cgaggcgggc gagggcaaga cactctccgc cgccgcgctc gccgccgcct tctgccacca
    24961 ggggcgcagc gttttcctgc tggagtgcaa ccccgtggct cccgccctgc acgagtggtt
    25021 cccccacagc gcccgccaca gctcctgggc gcatgacatg gaggcgctcc gctacggcaa
    25081 cacgcatctc ttcctcctgc ccgcgcacga cctccccgcc tacgccacca ccgagttgct
    25141 tgaaggctac cgctcctgga tagaccgcgc ccgccagcag gtggactgga tcatcctcga
    25201 cgccggtccc gtgctgcgta acttcgccga cgtggcaccc ctggccccgc tggccaccga
    25261 tgtgctcctg gtcaacaacc cgcgcgtcac caacgccggc aagctccgcg ccgccctcca
    25321 cctcctccag cccatgatgg ccagcagcgc cttccgcggc ctggtcatgc acggttgagg
    25381 tgtgactttg ctgccattcc gaaaaagccc cccgcgcatg ccgcagggtg cgattccctt
    25441 ttgccaagca ggtgtgcagc ggcacaatcc acactgatga aagccctgct tgccgtcctc
    25501 aatccggtga tggaaaaccg gaccagccgc accaacctgc gcgcgctggc aaacctgatc
    25561 ctcgtgctgg cggggctggt ggctgtgttc agcctgctct ttcatgtgct catggtgcgc
    25621 gaggggcagg agcactcgtg gatcacgggg ctgtactgga cgctgacggt gatgacgacc
    25681 ctgggcttcg gagacatcac ctttcagggc gatctggggc gcctgttctc cgtggtggtg
    25741 ctgtgctcgg gcgttgtctt catgctggtg ctgctgccct tcaccttcat cgagttcttt
    25801 tacgcgccct ggatgcgcgc ccaggcggcg gccaaggcgc cgcgcgagct gccggcctcc
    25861 accagcgggc atgtgatcct gaccgaccac gggccgctcg ccacgacgct gatccccatg
    25921 ctggaaaaat acggccatcc ctatgtggtg ctctgcccca cgcttcagga ggcgctggac
    25981 ctcgacgagc gcggcgtgcg cgtggctgtg ggtgatctcg acgaccctga gacttaccgc
    26041 cgcatgcgtg tcgagcaggc cgccatgctt gtggcgacac gagcggatgt catcaacacc
    26101 aacgccacct tcaccgcgcg cgagctcgcg gagcgcgtgc ccatcgtggc ctccgcctcc
    26161 tcggactccg cgcgcgatgt cctggaactg gcaggcgcca cgctcgtgct gcgcctggag
    26221 gagatgatgg gctccgcgct ggcgcggcgc gtgagcatcc gggactccga cgcgcatgtc
    26281 atcggcaatc tcgacggcct gctcatcgcc gaggctgccg cgcacggcac gccgctggcc
    26341 ggacagacgc tggcgcagag cggcctgcgc acccgcacgg gagtgagtgt cattggtgtc
    26401 tgggaccacg gacgcctcgc ggtggcggag gcggacatga tgatccggac ggacaccatc
    26461 ttcgtgctcg cgggcacgcg cgagcagctc gaccgctaca atgccgcctt tcccaacgac
    26521 accacgagca agccgcgcgt gctcatcatc ggcggcgggc gggtgggccg cgccaccagc
    26581 cgcgtgctgc gggatctggg catccagacg acgatcatcg aaaaaattcc cgagcgcgtg
    26641 gccgggcacc cggaggcggt catcggcgac gccactcaga tggatgtgct gaaaaaagcc
    26701 gccgcgcgcg aggccacgac actcatcatc accacgcacg atgacgacac caatgtggcg
    26761 ctgaccatct tcttccgccg cctgcgcagc aactggcaaa tcctggcccg cagcaccctg
    26821 gaccgtaatc tcaagaccct cctgcgagcc ggcgcggacc tggtgctctc ctactcctcc
    26881 atgggcgcga acacggtctt caacctgctc cgcggcggcg accacctgct gctggccgag
    26941 ggtgtgaacg tctttccctc accgatcccg gaatccgtcg ccggccgccg gcttggcgag
    27001 ctggagatcc gctcccagac aggctgcacg gtcattgctg tcgagtcgcg aggaaagcgc
    27061 gaaatcaatc ccgggccgga gctgctcatg ccagccggag gacgcatgtt cctgataggc
    27121 acgctcgagg cggaggaaaa attcctggag ctgcacaaac ccgcccagcc cctaaagcgc
    27181 ggccgccggc attgagcgac gcctcatcca gcggttgcca ctcatgcgcg catccaccat
    27241 tcttcagcaa tgtccgaagc agaaaacgcc acccgcatct ccgtccccgc cctgatccgg
    27301 ctcatgcggg aggagggtgt catcgagccg gaaaatccgc tgaccgggca gtgcgatctt
    27361 tttgcctgcg gtatggactc gctggcgatg atgcggctca tgattctcat cgagcgcgac
    27421 tttggcatcc gcatcccggc agagcaggtg acgcgcgacc gcttcgcctc ggcggacgcg
    27481 ctggcggaat ggcttggcag tcttcaccgc ccgcctttcc cggtatgagc ggttcatggg
    27541 atgtcatcgt ggccggaggc ggcagcgcgg gcaccgccg
//
LOCUS       DAOMYJ010000045        27315 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Prosthecobacter sp. isolate AMR_MDS_5629
            NODE_630_length_27315_cov_21.906016, whole genome shotgun sequence.
ACCESSION   DAOMYJ010000045 DAOMYJ010000000
VERSION     DAOMYJ010000045.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867850
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prosthecobacter sp. (wastewater metagenome)
  ORGANISM  Prosthecobacter sp.
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Verrucomicrobiaceae; Prosthecobacter.
REFERENCE   1  (bases 1 to 27315)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 27315)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.30
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:34:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,742
            CDSs (total)                      :: 4,684
            Genes (coding)                    :: 4,676
            CDSs (with protein)               :: 4,676
            Genes (RNA)                       :: 58
            tRNAs                             :: 55
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27315
                     /organism="Prosthecobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_630_length_27315_cov_21.906016"
                     /isolate="AMR_MDS_5629"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1965333"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..573
                     /locus_tag="PLP58_06855"
     CDS             <1..573
                     /locus_tag="PLP58_06855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ08271.1"
                     /translation="LYAGTLILLVALPRLALAGLSAWRAHAVMERRLRALGWRGYLER
                     ALRAAEGGGEVVTVIIHATDATPAHREVWARGVRERHGALVEPEMVHVPLGDEDDFAS
                     SWQPASSKVLVIFNLATTPEAEVQRRFVDDLRNALRTRRPDAELAVLLDATSIGNRWS
                     PEKKAGRERLWTEMLEGVADEVIIAAKRGG"
     gene            628..2094
                     /locus_tag="PLP58_06860"
     CDS             628..2094
                     /locus_tag="PLP58_06860"
                     /inference="COORDINATES: protein motif:HMM:NF023407.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase domain-containing protein"
                     /protein_id="HRJ08272.1"
                     /translation="MPPPPGITPPASDIVTLSLISHTNAGKTTLARTLLRRDVGEVRD
                     AAHITLFNESHTLLEDDARLLRLWDTPGFGDSARLLKRLKREKNPILWFLSQTWDRLT
                     DRPLWCSQQALKNVRDEADVVLYLVNATEPPEVAAHIRPEMEVLAWVGKPVLVLLNQT
                     GPALPPEEEAAELAAWRAWLNEFEQVRGVLALDAFARCWVQEDALMDALEPLLDGRKA
                     DAFRHLKRAWRRRNLEVFRHSARVLAELLTAAVLDGVDVRSETLLERIGIGRTELNKE
                     YHDARELLSTALADRMVRATNDLIRLHGLEGGALRRLGSLAHEQFQTRQKVPEAIWSV
                     VGSVAGGAMGGLIADLKMGGMTFGGGALVGGLLSGMGAYALIKSYNLVRGGDNRLHWS
                     REHFREQTRLALLAYLAVAHFGRGRGEWQESTEPAHWNQVVDEVVAEHEEGVDRLWKE
                     GVEKGAMPDALSKAALRLVNDCISCVLVRLYPSIHLRE"
     gene            2582..4900
                     /locus_tag="PLP58_06865"
     CDS             2582..4900
                     /locus_tag="PLP58_06865"
                     /inference="COORDINATES: protein motif:HMM:NF024753.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HRJ08273.1"
                     /translation="METPARFDSPPSIMALQRLLTCAAFLALLDSTRANDWTNWRGPL
                     QNGVSLEHYTGAGKLSETPAWTYPARGRGTSVICDGKVILWGYRGQTSDLIELLTCID
                     AKTGRKVWEHELPDYLSDSIYNRYSIGAPTIDPETRRIYLVSNAGTFYCFEFDGKKVF
                     ELPLMESLGRMTFPNSRVGSPVIEGDLVLVHYIFSNWGADGPAADRIYAFDKKSGELV
                     WWSLPGVSPPVDSSFSTPVLETRDGKRVAYYTTGCGHIVCVNVRNGKPYWKFPFCKNG
                     VNPSVVLHGGDKLIAIHNDENVDSSEKGRMTAIKLPSKLTAPVPPATESITLEPDAEL
                     WRFPIGATSSSPVVVGDTIYQLSDGGEIFAINVNTGVLIWKKKLSNANLHSSLLYVDG
                     LLYCPMMEGKLVVVKPGAADGEIIQEIKLEGECLGAPSVCDGVLYLTTTEKFYSFPIP
                     NSGIKTDPAPVVQIPAAGKAAALQIIPAEVTILAGEKQAFRIRSVDENGFVVSDDVKG
                     VKWESYIPPTAKVKATMNAAFNDAGELAVPAGSPPSAGAFKATSPDGLTGVIRGRGLK
                     NLPITEDFEGYTLTEEFPADPQGNAAYNFAYPPLPWIGARFKFQVMEKDGNKVFGKSF
                     DRLLFQRASVFIAPPHLKDYTMQADVLTEGNARSKSDIGFIHQRYLICLRGNAGKLEV
                     SSNLERLKQEAPFKLTANKWYTFKTRVDANDDGSGVVKGKVWEKGAAEPDAWTIEVKV
                     PRVHQNGSPGLFGFTPLNQRRMYIDNLSVTQN"
     gene            4930..6558
                     /locus_tag="PLP58_06870"
     CDS             4930..6558
                     /locus_tag="PLP58_06870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009959887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HRJ08274.1"
                     /translation="MTSKPTLLLALALAAGLNAADYPQWGGGNTRNMISDEKGLPIDI
                     DPGKKYGPRAAPKMAGRLRPNAEEANKAAGAEDIDMSTTKNCLWVAKLGSQTYGTPVI
                     ANGQVYVGTNNESPRDGKHTGDRGIVMVFDEYTGEFKWQLVSPKMGTGKVNDWEYLGI
                     CASPTIVGDKAYVPGNRCQIICMDVNGLSNGNQGMQDEAAFMAPLDKDGKATAPIEPG
                     KTDADILWVYDMYKELGVFQHNATSGFPLVLDGKVFVPTCNGVDWTHTNIPAPNSPSF
                     IMLDAESGELLGEQDSVASQRVLHCSWSSPNYIEAGGKKQIIFAAGDGWVYSLAPETK
                     KNDEDLDILVEHWRYDANPPEYRTNSAGEPIKYVEYDGPSEIIATPTIADGLIYVPIG
                     QDPEHGEGLGMLSCIDPATASGDISGKALWTFKGIERSISTPAVKDGLVYAADYTGRV
                     FCLDAKTGEQYWMFDTKGHIWASPLVADGKVWIGNEEGELFILAEGRELKEIKTIEFP
                     SSLLSSVVAANGAVYVTTHTHLYCFKEGAKPAAE"
     gene            6704..8983
                     /locus_tag="PLP58_06875"
     CDS             6704..8983
                     /locus_tag="PLP58_06875"
                     /inference="COORDINATES: protein motif:HMM:NF012428.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HRJ08275.1"
                     /translation="MPLRLVTELSETDRKLLHRAIDGFVPAKIFDAHTHLFHSRHFAE
                     GKRPVFLDEDRGYGMADFQAAMKLWMPGREVEGLFFGYPSAGNDRVGENAWVQSQIDA
                     STNSRALVLAAPMDDPDEVRRLMSTGVFVGIKPYRLYADVPDTKEAEIESFAPEWMWE
                     ACHDHDGIMVLHIMLADGITAPRNIEAIQRLCRKYPRCQLILAHIARSFNYRHAREGL
                     HHLIDLDNVVVDTSAVTQAGAFRAAVEILGPRRVLWGSDYMVSELRGSCITQGDGFTW
                     IHPDLGTDKLTIFGQYTTVGIESLLCLREACEDTGMTQGDLDDIFRENALRLLKPKTG
                     LSGPQLWEEAKTKISCGTGLLSKRAHLFDPQTWPSYFSRAKGASIWDLDGKRYTDFTG
                     GVGAILLGHADDEVNAAVKRRVNLGSYATLASPDEVKLADELLDLHPWAGKVRYARGG
                     GEALGLAVRIARAATGKSGIAFCGYHGWSDWYLAANLSDDAALDGHLLPGLQPLGVPR
                     ELAGTAVPFRYNDPASFQAALQKLDGRLAAVVMEPMRSDFPRDGFLQNVINACHAAGA
                     VFVLDEVTSGWRFGFPGAAPMLGITPDIAVYAKAMSNGYPAAAIVGKNEVMDASNKSF
                     ISSSYWTDGVGTAASLACIGKMRREGVQKHVWSLGERLQAGLREVASRHPALGLKISG
                     MPCASSLVFANPAAKPLMIREMLQRGYLMSSQLYVTWCHDDEKVAGMLAALDEALRIL
                     EVTPMEQSQDPQQGFARLA"
     gene            complement(8986..11955)
                     /locus_tag="PLP58_06880"
     CDS             complement(8986..11955)
                     /locus_tag="PLP58_06880"
                     /inference="COORDINATES: protein motif:HMM:TIGR02603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ08276.1"
                     /translation="MQQSQPAAPAAAARSWICFFGLCLASVQAPAQLANQPPVPEWLA
                     VSTEGAEAALGVEFQHQGTLLKAILLVAAETDTGVRVNGALAGNARTAPGEPAASLDV
                     TRHIKPGKNRLELTTRAPRVAALLELNGDLARKQWVATSAAWTSSAGKVRPQGAVDAG
                     AQANPFDLKKTFDAYNSWQLAKRGIQNQATDPAAFTLPAGFKVELVRSAQEDEDSWIA
                     LAFDPRGRVTLAREKTGLLRYDPASGGMEVIEETLKECRGLLYAHGALYAHANQSKGL
                     YRLRDLDGDGDFEDVKEIVRTEGGFGHGRNHFKLGPDGHLWVACGNNVLLPSPINEAS
                     PLKNHAWDQVLPNPWDGSMFDGTVEMPAGYIMRVSPDGSDIRIHAGGLRNPLDAAFNR
                     DGELFTFDADMERDVGASWYMPNRVLHLVSGADFGWRRGTGRLAAYQADTLPSVVDIG
                     LASPTAVFFGYGARFPEKYEEALYICDWSYGRIIAVHLTEKGASYTGRQETFISGRPL
                     NVTDGCVGPDGALWFITGGRGTQSGLYRVLWEGGANVQVARNDARATGTAAQPVRALR
                     HELESLHSGIPRGQEEAALAKILPNLDHADRHIRWAARLGLEQVPVKLWKKQIIEESN
                     GWRAILGCLALARVGESRDAPGIFTRLRSLSWRHLMDEQRLAALRALGVTIARQGDPD
                     DDQRARLLAWLEPLYPAAGSGLNHELCRLLVRMRSDKVIAKSMPLVRSAQAGEDLLFY
                     PLHLRYLAEGWTLAQRRLMFEALNSAEKLNGASTYFKAIQDTRSELAAALTPDQAAHL
                     AALIHPPKPVALSAHALPGHTFREWKLEDLENRLHEVGRGRSFEKGRDAAIRAQCVFC
                     HRVSNDPSLPAGIFGPELVQVSARFNRRDILDHTLNPSKVIDEKYRFITLTRLDGSTV
                     TGALESEDDERVILKPNPLAPDTIETGHSSIRERKESSLSPMPAGLLNALRAEQILDL
                     LAYIEAGGDAGHAVFRP"
     gene            12068..12679
                     /locus_tag="PLP58_06885"
     CDS             12068..12679
                     /locus_tag="PLP58_06885"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013352894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HRJ08277.1"
                     /translation="MAHTLPPLPYDKAALEPHIDAATMEIHHGRHHNAYVTNLNNAIA
                     GKADLEAMSIEDLCKNIASVPADIQGAVRNNGGGHFNHTLFWNIMGPNAGGAPTGALA
                     DAINAAFGSFDAFKEAFAKAGVTRFGSGWAWLVVKDGKLVITSTPNQDNPLMDGSGTP
                     ILGCDVWEHAYYLKYQNKRPDYIAAWWNVVNWDAVAANYAKAL"
     gene            12745..13407
                     /gene="eda"
                     /locus_tag="PLP58_06890"
     CDS             12745..13407
                     /gene="eda"
                     /locus_tag="PLP58_06890"
                     /EC_number="4.1.2.14"
                     /EC_number="4.1.3.16"
                     /inference="COORDINATES: protein motif:HMM:TIGR01182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008675 -
                     2-dehydro-3-deoxy-phosphogluconate aldolase activity
                     [Evidence IEA];
                     GO_function: GO:0008700 - 4-hydroxy-2-oxoglutarate
                     aldolase activity [Evidence IEA];
                     GO_process: GO:0009084 - glutamine family amino acid
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009255 - Entner-Doudoroff pathway through
                     6-phosphogluconate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HRJ08278.1"
                     /translation="MTLFPSSLQDRLHRGGVIAVLILEDAESAVPVARALVEGGVSAM
                     ELTLRTPAALEALRRIRAEVPEMIAGAGTILMPRQVDEAIGAGAAFGVAPGFNPRVVE
                     HALGSGLPFAPGVCTPTDVELALESGCKLMKLFPAEPCGGLLYLRAIAAPFAHCGARF
                     IPLGGIGPANAEAWLREPSVAALGGSWLAPREVVAKRDWHAITTAAREAAALVRRVRP
                     SD"
     gene            13437..14342
                     /locus_tag="PLP58_06895"
     CDS             13437..14342
                     /locus_tag="PLP58_06895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009958431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004609 - phosphatidylserine decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylserine decarboxylase"
                     /protein_id="HRJ08279.1"
                     /translation="MSAAPIQFYNRLTRRIETESVYGEAPLRFVYENPAGRLALRALV
                     RRRLFSRWYGARMDSPASRARIAPFIEKFGVDAGEFAGPVESFATFNEFFSRRLKPGA
                     RPLAGGDETLVFPADGRHLVIPDLSLETNFWVKGLRFDLRALLQDDAAARRYEGGAML
                     ISRLCPVDYHRFHFPCAGIPGASRLINGPLYSVSPIALARRPSILWENKRVVTQLDDT
                     PAGAVLLLEVGATCVGSIVQTCAPGVRAAKGDEKGYFRFGGSCFITIFEPGRARFAAD
                     LMQHSAAGREVYARMGDAAAEALTA"
     gene            complement(14532..14606)
                     /locus_tag="PLP58_06900"
     tRNA            complement(14532..14606)
                     /locus_tag="PLP58_06900"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(14572..14574),aa:Cys,seq:gca)
     gene            14755..18042
                     /locus_tag="PLP58_06905"
     CDS             14755..18042
                     /locus_tag="PLP58_06905"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HRJ08280.1"
                     /translation="MSHDEQSTIPTSPPDGSRPALTGVWQAPGVEQMRAMLPQYEVLE
                     MIGRGGMGAVYKGRQKSLNRLVAIKILPPGAADDEFKFTERFQNEARTMAAMNHPAIV
                     GVYDFGETADGLLYFIMEFVDGTDVQRMIQTSGRLSGEQALRITADVCDALAYAHKRG
                     VIHRDIKPANILIDTEGRVKVADFGLAKMQDASRTSALTRSNVAMGTPDYVAPEVLSP
                     GMTADHRADLYAVGVMLYQMLTGEVPRGMFKLPSERTPGADPRFDEVICKAMEQDREE
                     RYQTASDVRQALESIRTTPLAKDQNSGARAQRSRAMPKAPSASVSLGSFKWRAAAVGV
                     VALGAAAFFVVKGGKEPPAKAQPPSSAQADATAPVAVPDRTIDWINSPPATVSGWSPE
                     WNLRDGTLTPSAQLRWQFGLMKNGLVRLHVRLAPDYTEMDGNGPQLQLTIRSGSLNGP
                     QGRTGHMQFQTYVPNRTASLHFQDKTSPGLPVEQVVEGKPLQVSELGWREVQWEFRVV
                     GDEYSAWADGKKIASVRDARIASGYCMLEVRPGIQITLLETEGIEPVKATVPAPAVKE
                     TWTDQLAAREWSSPWIFEGEVLRTTDKNPTAYLGRMTDGALRIGFQPEPEKLLPGNNP
                     PLQISLRSTYYDEEAKVRGIYQIQIYPDRRISHLLYLAKGPPGQNDPPPEYLWKDRPW
                     PDAGAVADLEIRAEGGNLALLQDGKTIASAQDSRLSAGSCVMMASPGVRILKLQTLGV
                     AKENESVRSDMAAPLPPDIRHWQDVTGTVREKAKGMSELLVEADAVAHSTRGSPVMMP
                     LSPGSSRSLAVRLTHAGDGQVNWRSPSGGILYVLCQRTQTIIQRHAKDAAAPVKMVPD
                     VPHPASHSHAKPHELLVTLEGPLLRAWLDGRFICEVRDDAFTGGSCEIVISKWGDVRK
                     VETAELHPPASVWTDWLGPKLAAGGFAGGQWKREAGGISTDQVLAGFDVLPKDTRDGA
                     VKVTFVMRGSEGVLLDCRETKESGGRHLYNAQYTGKNLYISHSPPDGSATRRLINEPV
                     AEEIRSREEHTLEFHFIGEELTAILDGVHRVSTRHSLLKQGLCAVVLTQGVLVKKVEI
                     QALDSPR"
     gene            complement(18204..18923)
                     /locus_tag="PLP58_06910"
     CDS             complement(18204..18923)
                     /locus_tag="PLP58_06910"
                     /inference="COORDINATES: protein motif:HMM:NF016430.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HRJ08281.1"
                     /translation="MAQNPNFPTTSWTLIRKVREGGEKDAAAAMEEICRGYWYPIYAF
                     ARRAGHSIEDAEDLTQEVFASLVTHDSIRAAREDKGRMRTFMLGAAKNVFQKKLRRDR
                     AEKRGAGLAPLSLDELDAEDRYAREPADIRDPDSIFDRAWAEGVLSAAEEKLRADFAK
                     ADNLDGYAQLREFLPLGDHATPYAQAAKKLCLSEATLRLQIHRMRKRYGKLIEEEIAQ
                     TVSDPAAQKAELAHLMAVMGR"
     gene            19009..25812
                     /locus_tag="PLP58_06915"
     CDS             19009..25812
                     /locus_tag="PLP58_06915"
                     /inference="COORDINATES: protein motif:HMM:NF013508.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007154 - cell communication [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Calx-beta domain-containing protein"
                     /protein_id="HRJ08282.1"
                     /translation="MNPHLLLPFLAASLFLGSGGLAADPGTGRIVYQQFTDAFVNDLD
                     GRGVELVLVDAGGGNREVIAGSEFENYGHAALNADGTRVAFVAEQGGGGPKLWVMMAE
                     PHDPQDNPAVMVRENVSAQSRVCWSPDGRRIAFADAFDPQIYALEAVDGSGDVTPESG
                     DNLAVQVTSLNAQAQNPAWSPDGKFLAYSNGTALWALAVQDGSGALTPEAPGTHEPVP
                     LTSNLYSGEDFPAWSPDGTQLVFSSLQSGAPGGDRTDIAIRTVRQPQGVGGAILPAAQ
                     DGSDDGRLSLFRDLSGGPNPDYVHEAPNFVGEPSWSPDGSRVTYGHRFAGLPELNTVI
                     SVKPELEDAVINPGVALTEITNFGTGTPGGRRAVFQPAAPTGGGVLTAPENFVVSPGE
                     AGQPWSFTATLDSDAPGLFLRIQSTLTPDIEGSWIDLPGGGQMTRDGFFWSLETAVIP
                     AGTRYFRVIAAATGLQDSKTVEPQSYDVVSTAGRILYTRGSVLDRELVLMEADGSGKT
                     VLVSGGIFNVQQCALSADGGHAAFTTLAGKLFVMKAEPMDSETNEPVDILEAAAVGPM
                     FGSGITWSPFGPHLAFAGTDSRVHVIEVLDDGGNINPFNGSSVPLVDVGGTLSGAPNP
                     AWSPDGRHLALLGANSIELLEVADVAGNITTGGATNMPVPLTLQSDFTSTKTHAAWSP
                     DGQTIAFVERAISGDQAKISLLTAFDAFGTLTPEIAPTQENAGNPRQELHAMTGAPQV
                     KTVSWSPDGALLALETENNGEHRVEIIRPAELDGSNPRIPLTSQGTDGDAFQPSFQQP
                     VSDRALPAIVAFQAPTYAGNEDGDPIIVRVMRTGSVSGELEVDFTVTGGTAEDGLDYT
                     LTESPLVFADGQTEAQILVTPLPDSLDDEGDETIELELVSTSSGALGGITSTLVTLND
                     SDSNQLPFTPAAAEIVVVVNGKTLKKGGAKTGDRFAFTVRQDLGALLPGLDVKIQTSA
                     TPNDPASWTNVPNLIMKRATKTSMTWKGSVTASVLPHGTLHFRTRSYATGHRSNAGPV
                     AGPFKITPAPMLVLTTWVVTDSHPNGLTVKPGEFITYHLRCDNIGAAPAKKVVLNSLI
                     PAQTRLDSASHDNAASPGYFKRVTSSKGVITDVQWNVGDIQAGQHVSEFVTVQVNQEV
                     EFARLVLNNRLTYKGTGVKEFFTPSLATQIESPLLLTLTRDKNIVQAGDLITYTLTAT
                     NDASYQITGGQVRNQIPDGTRLYSVRQGDGAGNYLGTELAHTLLEESPQGMTRPGYTL
                     HNRTLLWNIGTLAGGSQRQLRYTVRVAYDLFEKIARNGESYNTEIRNLNFDFTGTPPS
                     GGVIAAYNGAIPSDRITRNPVSAETPAQRPELGLVKKAVSDTVATVGGKEAAAVLADE
                     WRRVTYKIEAWNYGNITAEDTVIYDVLPQGTDFDMDMSLTGSHAEDILTTDEPHGFTA
                     GEKIIFTALTGGKGLAVNKIYFVSSVPSADPFTVSTSAGGPVLNFTTDVSVGDARRIA
                     YDWPRFMSRFTLDGAPFAFPDGFTFFDEKGAVILPGGEAFVDTNGNGKVDKGEFVDGN
                     GNGKYDGPDKVRSFEFRLGHLTQMTRPAVRTLSYRVKVRPTVKSGTQITAFGSSSVKR
                     GEGLELVSPDFIYPLSGTPELLHTRVVSRMTWLMEPPHPLDAGYVTDDPLLRAVPHLI
                     RFRNTSSDLYALDAKLRIPVPQGFEVTAPGGSLQKFDAKLDAKLDKSPGTFVLDIGEV
                     APGQQIERQITSRFLPPMPAGWTDKKGVLLQTQAFNHANVVSTRVSSEPPSSGFSLLS
                     TAASEPRFEIKFDARAANVSALPVMKTHTDPRVFVGRIAPAAVQAGSEMDIFIFYGNH
                     GGVRTDNGEIAMQIPWGTSLVAAEPICYNPEAGSPNQTQGFSRGWFTIKTEKGPKRRP
                     QDGNAGVVFFKFGNMAPAEVGVVRVRLFVTQDFPMGFLTDQSLYIKFDGVAGKSPPPL
                     SVQVLRNTSGATDWWQAAGNFFDKLGSDINAALRNIFGGGNGEFRYDRAIVTLGGADF
                     VHLASGPIIIPLGSSRSAIIAEYDDIVGRMPSSAIMLHGETGVRNGYLAMAAGLTSAQ
                     QVFVPGSIQQDRTVHSILAELTLPSSIVAAGGGNIVAAGGGNIVAGGGGNIVAGGGGN
                     VVANDGAGFLPGSINFSIPGVNPGIVAAGGGNIVAAGGGNIVAAGGGNIVAAGGGNIV
                     SAGGGNLISVDGLKFDISKMSALIAKIALIDPAKLLDAGNLKLIGQDGAGLIGQDGAG
                     LIGQDGAGLIGQDGAGFNF"
     gene            complement(25828..26679)
                     /locus_tag="PLP58_06920"
     CDS             complement(25828..26679)
                     /locus_tag="PLP58_06920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009965050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00282 family metallophosphoesterase"
                     /protein_id="HRJ08283.1"
                     /translation="MISEAAAATESGSPTASDHFRILFLGDVVGEPGRKAVTALLPVL
                     KQELAVDFIIVNGENSAGGRGITPKIAIGLMRAGAAVITTGDHIWDQKEIVPFLAEEP
                     RVLRPLNYPEGAPGQGSLVLETKKGCKVGVINLQGRVFMRDQLENPFTVITREVERMR
                     LDTPIIFVDFHAEATSEKVAMGWHLDGKVTAVIGTHTHVPTADERVLPRGTAYQSDAG
                     MCGPLESVIGSQIEPVLEKFHTQLPARYGVGRGTVRVNGVLVTLDPATGRALAIERVA
                     RMWEGHN"
     gene            26729..27301
                     /locus_tag="PLP58_06925"
     CDS             26729..27301
                     /locus_tag="PLP58_06925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ08284.1"
                     /translation="MTLLFRAALTVCVLAGSLAAQTPALPLSAPPSPADAPSPEVLAL
                     RLSKTTPLGKKPDWSGLDAYQGVFTRAEFELAWREIYSTESGLPAPLKILADTVEVPT
                     GQPLTPVKHIAFRPEGAPRPPQPGQVRSIVKNPWLARTLPMPPQVGHWVGLVPSLAPV
                     PVHASHAMLVGTRISATLSVNASLRLISML"
BASE COUNT         5060 a         8806 c         8499 g         4950 t
ORIGIN      
        1 ctctacgccg gcacgctgat cctgctggtg gctctgccga ggctggcgct ggccggcctg
       61 tcggcatgga gggcgcacgc ggtgatggag aggcgcctgc gcgcgctggg ctggcgcggt
      121 tatctggagc gcgcgctgcg cgctgcggaa ggcggaggcg aggtggtgac ggtgatcatt
      181 cacgccacgg atgcaacgcc cgcgcaccgc gaggtgtggg cgcgcggtgt gcgcgagcgg
      241 cacggcgcgc tggtggagcc ggagatggtt catgtgccgc tgggggatga ggacgatttt
      301 gcctcgtcat ggcagcctgc gtcctcgaag gtgctggtca ttttcaatct ggcgaccact
      361 cccgaggcgg aggtgcagcg gcggtttgtg gacgatctga gaaacgcgct gcgcacccgc
      421 cggccggacg cggagctggc ggtgctgctg gatgcgacga gcatcggcaa ccgctggtcg
      481 ccggagaaaa aagcggggcg cgagcgtttg tggacggaga tgctggaggg tgtggccgac
      541 gaggtgatca tcgcggcaaa acgcggtgga tgaaccaatc accgtccttg ccatctgaac
      601 cagactgctt cacttccccg acactccatg ccaccaccgc caggcatcac accgcccgcc
      661 tcggacatcg tcacgctcag cctgatctcg cacacgaacg cgggcaagac aacgctggcg
      721 cgcacgctgc tgcgcaggga cgtgggcgag gtgcgcgatg cggcgcatat cacgcttttc
      781 aacgagtccc acacgctgct ggaggatgac gcgcggctgc tgcggctgtg ggacacgccc
      841 ggcttcggcg acagcgcgcg tcttttgaaa agactcaaac gggagaagaa ccccattctc
      901 tggtttcttt cgcagacctg ggaccggctg acggaccgcc cgctctggtg cagccagcag
      961 gcgttgaaaa atgtccgcga tgaggcggac gtggtgcttt atctggtgaa cgccaccgag
     1021 ccgcccgagg tggccgcgca catccgcccg gagatggagg tgctggcctg ggtgggaaag
     1081 ccggtgctgg tgctgctgaa ccagaccggc cccgctttac cgcccgagga ggaagcagcg
     1141 gagctagcgg cgtggagggc gtggctgaat gagttcgagc aagtgagggg cgtgctggcg
     1201 ctggacgcct tcgcgcgctg ctgggttcag gaggatgctt tgatggatgc gctggagccg
     1261 ctgctcgacg ggcgcaaggc ggatgccttc aggcacctca aacgcgcctg gcgcagacgg
     1321 aatctggagg tcttccgcca ttccgcgcgc gtgctggccg agctgctcac cgccgccgtg
     1381 ctggacggtg tggatgtgcg ctccgagaca ctgctggagc gcattggcat cggccgcacg
     1441 gaattgaaca aggaatacca cgacgcgcgc gaactgctct ccaccgccct ggccgaccgc
     1501 atggtgcgcg cgacgaacga tctcatccgc ctgcacggcc ttgagggtgg cgccttgcgc
     1561 aggctcgggt cgctggccca tgagcagttc cagacgcgcc agaaagtgcc ggaggccatc
     1621 tggagcgtcg tgggcagcgt ggccggcggg gcgatgggcg gcttgattgc cgatctgaaa
     1681 atgggcggca tgactttcgg tggtggcgcg ctggtcggcg gccttctcag cggcatgggt
     1741 gcctatgctt tgatcaaaag ctacaacctc gtgcgcggag gcgacaaccg cctgcactgg
     1801 tcgcgcgagc attttcgcga gcagacacgg ctggcgttgc tcgcctatct ggcggtggcg
     1861 catttcggac gcggacgcgg cgaatggcag gagagcacgg agcccgcgca ttggaaccag
     1921 gtggtggacg aggtcgtggc cgagcacgag gaaggcgtgg accgtctttg gaaggagggc
     1981 gtcgagaaag gcgccatgcc tgacgcgctc tccaaagcag cgctgcgcct ggtcaatgac
     2041 tgcatctcct gcgtgctggt gcgcctttac ccgagcatcc acctgcgcga gtgaccgccc
     2101 ccaaacaaga gaccctccgg catgcggaac cggagggtct gtttggcgat ccagcaacca
     2161 acaacagtga ggatcgttgg cacgaaagtt tgagctgagg aaagctgcaa cttcgcggtc
     2221 catgagcgga ttgctccact ggctggacgg cgggaggtta aaaagttttg ggggcggagt
     2281 caacccggag ggcgattttg ggcggtaatt ttgcaaaact ctaggttatt ctcaaaaatg
     2341 ataggtttca gaattgagaa aaatctgaaa attttagaag aaaccgggtt gacaggtatg
     2401 tgagtaaaga actgttcgtg tgaataaatc caataacttc tgctgcgcac cctccttcaa
     2461 agtcagtctc ccggaccgaa agacgccgcg aatgccttcc gtttcccctt ccccatttga
     2521 gtgtccccgg cgcggaggcc aatagacggt tggacaaacc gattgaactc ccttccgccg
     2581 catggaaact cccgcccgtt tcgattcacc tccatccatc atggccctcc aacgcctgct
     2641 cacatgcgcc gccttcctgg cgctcctcga ctccacccgc gccaacgact ggaccaactg
     2701 gcgcggcccc ctgcaaaacg gcgtctcgct ggagcattac acgggcgccg gaaaactttc
     2761 cgagactccc gcctggacct acccggcccg cggacgcggc acatccgtca tctgcgatgg
     2821 caaagtcatc ctatggggtt atcgcgggca gacttccgac ctgatcgaac tgctcacctg
     2881 cattgacgca aaaaccggcc ggaaagtgtg ggagcacgag ttgcccgact acctcagcga
     2941 ctcgatctac aaccgctact ccatcggcgc cccgaccatt gatcccgaga ccaggcggat
     3001 ctatctcgtg agcaatgccg gcactttcta ttgcttcgag ttcgacggga aaaaggtctt
     3061 cgagctgccg ctcatggaaa gcctcggtcg catgactttc ccgaacagcc gcgtcggcag
     3121 cccggtcatc gagggagact tagttcttgt gcattacatc ttctccaact ggggcgccga
     3181 cggccctgcc gcggatcgca tatatgcctt tgacaaaaaa tcaggcgaac tcgtctggtg
     3241 gtccctgccc ggagtttccc cgccggtgga cagttccttc tccacccccg tgctggagac
     3301 tcgcgacggc aagcgcgtcg cctattacac caccggctgc ggccacatcg tctgcgtcaa
     3361 cgtccgcaac ggcaaaccct actggaaatt tcccttctgc aaaaacggcg tcaatcccag
     3421 cgtcgtgctt cacggcggcg acaagcttat cgccattcac aatgacgaaa acgtggacag
     3481 cagcgagaaa ggccggatga ccgccatcaa actgccctcg aagctcaccg cgcccgtccc
     3541 gccagccacc gagtccatca cactcgagcc tgatgcggaa ctttggcgct tccccatcgg
     3601 cgccaccagc tcctcccccg tggtcgtcgg agacaccatc taccagcttt ccgacggcgg
     3661 tgagatcttc gccatcaacg tcaacaccgg cgtcctgatc tggaagaaaa aactcagcaa
     3721 cgccaacctc cactcctccc tgctctatgt ggacggtctc ctctattgcc cgatgatgga
     3781 gggcaagctc gtcgtcgtga aacccggcgc cgccgatgga gagatcatcc aggagatcaa
     3841 gctcgaaggc gaatgcctcg gcgcgccctc cgtctgcgac ggcgtgctct acctcaccac
     3901 caccgaaaag ttttacagct tccccattcc gaacagcggc atcaagaccg accccgcccc
     3961 cgtcgtgcaa atccccgccg caggcaaggc ggccgccctc cagatcattc ccgccgaggt
     4021 caccatcctg gccggggaaa aacaggcctt ccgcatccgc agcgtggatg aaaacggctt
     4081 cgtcgtcagc gatgacgtca aaggcgttaa atgggaaagc tacatcccgc ccaccgccaa
     4141 agtgaaggcc accatgaacg ccgccttcaa tgacgctgga gaactcgccg tccccgccgg
     4201 ctcccctccc agcgccggcg ccttcaaagc cacatctcca gacggactca ccggcgtcat
     4261 ccgcggacgc gggttgaaaa acctgcccat caccgaggac ttcgaaggct acaccctcac
     4321 cgaggaattc cccgccgacc cgcaaggcaa cgcggcctac aacttcgcct accccccgct
     4381 gccctggatt ggagcccgct tcaaattcca ggtcatggag aaggacggca acaaagtctt
     4441 cggcaaaagt ttcgaccgcc ttctctttca acgcgcctcc gttttcatcg ccccgccgca
     4501 tttgaaagac tataccatgc aggccgacgt gctcaccgag ggcaacgccc gttcaaagtc
     4561 cgacatcggc ttcatccacc agcgctacct catctgcctg cgcggcaacg ccggcaaact
     4621 ggaggtatcc tcaaacctgg agcggctcaa acaggaggca cccttcaagc tcaccgccaa
     4681 caaatggtac accttcaaaa cccgggttga cgccaatgat gacggctccg gcgtggtgaa
     4741 aggcaaagtt tgggagaagg gcgccgccga gccggatgcc tggaccatcg aggtcaaggt
     4801 cccccgcgtt catcaaaacg gctcccccgg cctcttcggt ttcacccccc tcaaccagcg
     4861 ccgcatgtac atagacaacc tcagcgtgac gcagaactga aggttcaata caaccctccg
     4921 ccaactatca tgacatccaa acccaccctc ctcctcgcgc tggccctcgc cgccggcctg
     4981 aacgccgccg attaccctca atggggcggc ggcaacaccc gcaacatgat ctccgacgag
     5041 aaaggcctgc cgattgacat cgatcccggc aaaaagtacg gcccccgtgc cgcgccaaaa
     5101 atggcaggac gcctgcgtcc gaacgcggaa gaggccaaca aggccgccgg tgccgaggac
     5161 attgacatga gcaccaccaa gaactgcctc tgggtggcca agctcggttc gcagacctat
     5221 ggcactcccg tcatcgccaa cggccaggtt tatgtcggca caaacaacga gtccccccgg
     5281 gatggcaagc acacaggcga ccgcggcatc gtcatggttt ttgacgagta taccggcgag
     5341 ttcaagtggc agcttgtctc cccgaaaatg ggcactggaa aggttaatga ctgggaatat
     5401 ctcggcattt gcgccagccc caccattgtc ggcgacaaag cctacgttcc aggcaaccgc
     5461 tgccagatca tctgcatgga tgtcaatggc ctcagcaacg gcaaccaggg catgcaggac
     5521 gaggcggcct tcatggcccc gctcgacaag gacggcaaag ccacagcccc catcgaaccg
     5581 ggcaaaaccg acgccgacat cctctgggtg tatgacatgt acaaggaact cggcgtcttc
     5641 cagcacaatg ccacctccgg tttcccgctg gttcttgacg gcaaagtctt cgtgcccacc
     5701 tgcaacggcg tggactggac ccacaccaac attcccgccc ccaacagccc cagcttcatc
     5761 atgctcgacg ccgaaagcgg cgagttgctc ggcgagcagg acagcgtcgc ctcccagcgc
     5821 gtgctccact gctcatggtc ctccccgaac tacattgagg ccggcggtaa aaagcagatc
     5881 atcttcgccg ccggtgacgg ctgggtttac tccctggctc ccgaaaccaa aaagaacgac
     5941 gaagacctcg acatcctcgt cgagcactgg cgctatgacg ccaacccgcc cgaataccgc
     6001 accaactccg cgggcgagcc catcaaatat gtggagtacg acggcccctc cgagatcatc
     6061 gccacgccta ccatcgccga cggcctcatc tacgttccca tcggccagga tcccgagcac
     6121 ggcgaggggc tcggcatgct ctcctgcata gaccccgcga cagccagtgg tgacatcagc
     6181 ggcaaggccc tctggacgtt caaaggcatc gagcgctcca tctccacccc ggcggtcaag
     6241 gacggcctcg tctatgccgc cgactacact ggccgagtct tctgcctcga tgccaaaacc
     6301 ggcgagcaat actggatgtt cgacacaaag ggccatatct gggccagccc gctcgtcgcc
     6361 gacggcaaag tctggatcgg caatgaggag ggagagcttt tcatcctcgc cgagggcagg
     6421 gagttgaagg agatcaagac catcgagttc ccctcatccc tcctcagcag cgtcgttgcc
     6481 gccaacggag ccgtgtatgt gaccacccac acgcatctct attgcttcaa ggaaggcgcc
     6541 aaacccgccg ctgaataatg ccgtgaatgc cggattcgcg cccgcgctcg ttccggcagt
     6601 cgcctcgcac tcccgtctca tcagcccgcg ccgcgtgttt cgcagtgcgg gctgagtatt
     6661 ttctggcttg tggcgcggtg ccgcttccat tcactcattc gtcatgccac tccgcctcgt
     6721 caccgaacta agcgaaaccg accgcaagct gctgcatcgc gccattgacg gctttgtgcc
     6781 cgcgaagatc ttcgatgcgc acacgcacct gtttcacagc cgtcattttg ccgaaggaaa
     6841 gcgccccgtg ttcctcgatg aggatcgtgg ctacggcatg gccgattttc aggccgcgat
     6901 gaagctctgg atgccgggtc gtgaggtcga aggccttttc ttcggctatc cgagtgctgg
     6961 caatgatcgc gtcggcgaaa acgcctgggt gcagtcgcaa atcgacgcga gcacgaactc
     7021 acgcgcactt gtgctcgccg ctccgatgga tgatccggac gaagtgcgtc gcctcatgag
     7081 caccggcgtc tttgtcggca tcaaaccgta tcgcctgtat gccgatgtgc cggacacgaa
     7141 ggaggcggag atcgaatcct tcgcgccgga gtggatgtgg gaggcttgcc acgatcacga
     7201 tggcatcatg gtgctgcaca tcatgctcgc cgatggcatc accgctccgc gcaacatcga
     7261 ggcgattcag cgtctctgcc gcaaataccc gcgctgccag ctcatcctcg ctcacatcgc
     7321 tcgcagcttt aactaccgcc atgcccgcga gggcctgcat caccttatcg atctcgacaa
     7381 cgtcgtcgtg gacacttccg ccgtgacgca agccggtgcc ttccgcgccg cggttgagat
     7441 tctggggccg aggcgcgtgc tttggggcag cgattacatg gtcagtgagc ttcgcggctc
     7501 ctgcatcacg caaggcgatg gcttcacttg gattcatcct gatctcggca cggataaact
     7561 gaccatcttt ggccaataca cgacggtcgg catcgaatcg ctcctctgcc tccgcgaagc
     7621 ctgcgaagac accggcatga cgcagggcga cctcgacgac atcttccgcg agaacgcgct
     7681 gcggctgttg aagccgaaaa ccgggctttc aggtccccaa ctctgggaag aagccaaaac
     7741 gaagatctcc tgcggcaccg gcctgctttc caaacgcgcc catctcttcg atccacaaac
     7801 ctggccgtcg tatttttcgc gggcgaaggg cgcgagcatc tgggatctcg atggcaaacg
     7861 ctacaccgac ttcaccggcg gtgtcggagc cattttgctc ggtcatgccg atgacgaagt
     7921 gaatgccgcg gtgaagcgtc gcgtgaatct cggcagctat gccacgctcg cttcaccgga
     7981 tgaggtgaag ctcgccgatg agttgctcga tttgcatccc tgggccggca aggtgcgcta
     8041 cgcccgcggc ggtggcgagg cgctcggtct cgcggtgcgc atcgcgcgcg cggccaccgg
     8101 gaaaagcggc atcgccttct gcggttatca tggttggagc gattggtatc tcgccgcgaa
     8161 cctctccgat gatgccgcgc tcgacggaca cctgctgccg ggccttcaac cgcttggcgt
     8221 gccacgcgaa ctggcgggca cggcggtgcc gtttcgctac aacgatcccg cgtctttcca
     8281 ggccgcgttg cagaagctcg atggcaggct cgccgccgtg gtgatggagc cgatgcgcag
     8341 cgacttcccg cgcgatggct tcctgcaaaa cgtgatcaat gcctgccatg ccgccggagc
     8401 ggtgtttgtg ctcgatgaag tcaccagcgg ctggcgattc ggcttcccag gtgccgcgcc
     8461 gatgctaggc atcacgccgg acatcgccgt gtatgcgaag gcgatgagca acggctatcc
     8521 cgccgcagcc atcgtgggca aaaacgaggt catggacgcc tcgaataaga gtttcatttc
     8581 cagcagctac tggaccgatg gcgtcggcac cgccgcttcg ctggcctgca tcggcaaaat
     8641 gaggcgcgaa ggcgtgcaaa agcatgtgtg gagcctcggt gagcgtttgc aggccggatt
     8701 gcgcgaggtg gcctcgcggc atcccgcgct cggtttgaag atcagcggca tgccctgcgc
     8761 gtcatcgctc gtttttgcca atccagccgc gaagccgctt atgatccgcg aaatgctcca
     8821 gcgtggctat ttgatgtcga gccagcttta tgtgacctgg tgccacgacg atgaaaaggt
     8881 cgctggaatg ctcgccgcgc tggatgaagc tctccgcatt ctcgaagtga ccccgatgga
     8941 acagtcacaa gacccccagc agggattcgc tcgattggcc taacatcaag gacggaaaac
     9001 agcgtgtccc gcatctccgc ccgcctcgat ataggccagc aaatcgagaa tctgctccgc
     9061 gcgcaaagca ttgagaagcc cggctggcat gggtgaaaga ctggattcct tccgctcgcg
     9121 gatgctgctg tgaccggtct cgatcgtgtc gggcgcgagc gggtttggtt ttaaaatcac
     9181 gcgctcatcg tcctccgact ccagcgcgcc ggtgacagtg ctgccatcca agcgggtgag
     9241 ggtgatgaaa cgatattttt catcaatgac cttcgagggg ttcagcgtgt ggtcgagaat
     9301 gtcgcggcgg ttgaagcgcg cggacacctg caccagttcg gggccgaaaa ttcctgccgg
     9361 cagggacggg tcattgctga cacggtggca gaagacgcat tgcgcgcgga tggcggcatc
     9421 gcggcccttt tcaaaggagc ggccgcgccc gacttcgtgc aggcggtttt ccaggtcctc
     9481 cagcttccac tcgcggaagg tgtgtccggg cagcgcgtgg gccgaaagcg cgactggctt
     9541 gggcgggtgg atgagagcgg cgagatgagc ggcctgatcc ggcgtcaagg cggcggccag
     9601 ttcgctgcgc gtgtcctgaa tggctttgaa gtaggtgctg gcgccgttga gcttctcggc
     9661 gctgttgagg gcctcaaaca tcagccgccg ctgggccagc gtccagccct cggcgaggta
     9721 acgcaggtgc agtggataaa aaagcaggtc ctcccccgct tgcgcagagc gcaccagggg
     9781 catggatttg gcgatcacct tgtccgagcg catacgcaca agcagccggc acagctcgtg
     9841 gttcagcccg cttcccgccg ccggatacaa aggctccagc catgcgagca gccgcgcgcg
     9901 ctggtcgtca tcaggatcgc cctggcgcgc gatggtgacg cccagcgcgc gcaacgcggc
     9961 aaggcgctgc tcgtccatga gatgccgcca tgaaagcgag cgcaggcgtg tgaaaatgcc
    10021 gggcgcatct ctggactcac ccacacgcgc cagggccagg cagccgagga tggcgcgcca
    10081 gccgttcgat tcctcaatga tctgcttttt ccaaagcttt accggcacct gctccagccc
    10141 aagccgtgcc gcccagcgga tgtggcggtc ggcgtggtcg agattcggga ggatctttgc
    10201 caaagccgcc tcttcctgcc cgcgcggaat gccgctgtgg aggctctcca gctcatgccg
    10261 tagcgcgcgc acgggttggg cagccgtccc ggttgcccgt gcatcgttgc gtgcgacttg
    10321 gacgttcgcg ccaccctccc acaacacgcg gtaaagcccg ctctgcgtcc cgcgcccgcc
    10381 ggtgatgaac cacagcgcgc catccgggcc gacgcagccg tccgtgacat tgagcgggcg
    10441 tccgctgatg aaggtctcct ggcgtcctgt gtagctggcg cctttctcag tgagatgcac
    10501 ggcgatgatg cgaccgtaag accagtcgca gatataaagc gcctcctcgt atttctccgg
    10561 gaagcgagcg ccgtagccga agaacacggc ggtgggcgag gccaggccga tgtccaccac
    10621 gcttggcaaa gtgtccgcct gataagccgc caagcgtccg gtgccgcgcc gccagccgaa
    10681 gtccgcgccg gagacgagat gcagcacacg gtttggcatg taccaggagg cgcccacatc
    10741 gcgctccatg tcggcgtcga aggtgaaaag ctcgccgtca cggttgaaag ccgcatccag
    10801 cggattgcgc aggccgccgg catggatgcg gatgtccgat ccgtccgggc tgacgcgcat
    10861 gatgtatccg gcgggcatct ccacggtgcc gtcgaacatg ctgccatccc acgggtttgg
    10921 gagcacctgg tcccaggcgt ggtttttcag cggcgatgcc tcgttgatgg ggctgggcag
    10981 cagcacattg ttgccgcagg cgacccagag atggccgtcc gggcccagct tgaagtgatt
    11041 cctgccgtgg ccgaagccgc cttcggtgcg gacgatctcc ttcacatcct caaagtcgcc
    11101 gtcgccatcc aagtcgcgca ggcggtaaag gcccttgctt tggttggcat gggcataaag
    11161 cgcgccgtgc gcgtaaagca ggccgcggca ttccttgagc gtttcctcaa tgacctccat
    11221 gccgcccgag gcgggatcat agcggagcag accggttttt tcccgcgcca gggtgacgcg
    11281 ccctcgcggg tcaaaagcca gcgcgatcca ggaatcctcg tcttcttgag ccgagcgcac
    11341 cagctccacc ttgaagcccg ccggcagggt gaaggccgcc ggatcggtgg cctggttttg
    11401 aatgccgcgt ttggcgagct gccacgagtt gtaggcgtcg aaggttttct tcaaatcaaa
    11461 agggttggcc tgcgcgccgg cgtccaccgc gccctgcgga cgcactttgc ccgcgctgct
    11521 ggtccatgcg gcgctcgtcg ccacccactg cttgcgcgcg aggtcgccgt tcagctccag
    11581 cagcgccgcc acacgcggag cgcgcgtggt gagttccagc cggtttttgc cgggtttgat
    11641 gtggcgggtc acatccaggc tcgccgccgg ctcgccggga gcggtgcgcg catttcccgc
    11701 gagcgcgcca ttgacccgca ctccggtgtc cgtctccgcc gccaccagca ggatggcctt
    11761 gagcaaagtc ccctgatgct ggaattcaac gccgagggcg gcctccgcgc cctcggtgga
    11821 gactgccagc cattcaggga cgggcggctg gttggcgagc tgcgcgggcg cttgaactga
    11881 cgccagacac aggccgaaaa aacaaatcca ggaccgggcg gcagcggcag gagcggcggg
    11941 ttgggactgt tgcatgggca acgaacacac atttcccccg gcccctttcg aacttcgaat
    12001 ttcgaacttc gaatttgccc cggcgcgtat gaggatttac ctcccgtctc gaacccatac
    12061 caagaacatg gcccacacac tcccccccct gccttacgac aaagcggcgc tcgaacccca
    12121 cattgacgcc gccacgatgg agatccacca cggacgccat cacaacgcct atgtcaccaa
    12181 cctgaacaac gccatcgccg gcaaggccga tctcgaagcc atgtccatcg aggacctgtg
    12241 caaaaacatc gccagtgtgc ccgcagacat ccagggcgcc gtgcgcaaca acggcggcgg
    12301 ccactttaat cacacgctct tctggaacat catgggtccc aacgcgggcg gagctcccac
    12361 aggcgccctc gccgacgcga tcaacgccgc ttttggcagc ttcgacgctt tcaaggaggc
    12421 tttcgccaag gccggtgtca cccgctttgg ctccggctgg gcctggctgg tggtgaaaga
    12481 cggcaaactc gtcatcacat ccactccgaa ccaggacaat ccgctcatgg acggatccgg
    12541 cacgcccatt ctcggctgcg atgtctggga gcacgcctac tacctcaagt atcagaacaa
    12601 gcgccccgac tacatcgccg cctggtggaa tgtggtgaac tgggacgccg tcgctgccaa
    12661 ctacgccaag gcgctctgat ttttccccgt tcatggcggc gccgcatctc ttggcggcgc
    12721 cgctttttta tgtccgcccg gcccatgacg ctttttccct catctctcca ggaccggctg
    12781 caccggggcg gtgtcatcgc cgtgctcatc ctcgaagacg cggagtccgc cgtgccggtg
    12841 gcgcgcgccc tggttgaggg cggtgtcagc gccatggagc tgacactgcg cacgccggcg
    12901 gcgcttgagg cgctgcggcg catccgtgcc gaggtgccgg aaatgatcgc cggagccggc
    12961 acgatcctca tgccccggca ggtggacgag gccatcggcg cgggcgcggc ctttggcgtg
    13021 gcgccgggat tcaatcctcg cgtggtggag catgcgcttg gctccgggct gcccttcgcg
    13081 cccggagtct gcacaccgac ggacgttgag ctggccttgg agtccgggtg caaactcatg
    13141 aaactcttcc ccgccgagcc ctgcggaggc ctgctgtacc tgcgcgccat cgccgcgccc
    13201 ttcgcgcatt gcggcgcgag gttcatcccc ctcggcggca tcggccccgc aaacgcggaa
    13261 gcctggctgc gcgagccctc cgtggctgcc ctcggcggtt cctggctcgc gccgcgcgag
    13321 gtcgtggcaa agcgtgactg gcacgccatc accaccgccg cgcgcgaggc ggcggcattg
    13381 gtgcggcggg tgcgaccgtc agactgaagc gcgggcccac tttggaccac ctcatcatgt
    13441 ccgccgcacc gatccagttc tacaaccgcc tcacccgccg gatcgagacc gagtccgtct
    13501 atggcgaggc acccctgcgc tttgtctatg aaaaccccgc cggacggctg gccctgcgcg
    13561 ctctggtgag gcggcggctt ttctcccgct ggtatggtgc gcgcatggac tcgcccgcga
    13621 gccgggcgcg gatcgcgccc ttcatcgaaa aattcggcgt ggatgccggc gagttcgccg
    13681 ggccggtgga gagcttcgcc accttcaacg aattcttctc ccgcaggctc aagcccggcg
    13741 cgcgtcccct ggcgggcggt gatgaaaccc tcgtcttccc cgccgacggc cgccatctag
    13801 tcatcccgga cctttctttg gagacgaact tctgggtcaa aggcttgcgc ttcgatctgc
    13861 gcgcgctgct ccaggatgac gccgccgcgc gccggtatga aggcggggcc atgctcatct
    13921 cccgtctctg ccccgtggac tatcaccgct tccacttccc ctgcgcgggc attcccggcg
    13981 ccagccggct catcaacggc ccgctctact ccgtgagtcc catcgccctc gcgcggcggc
    14041 cctccatttt gtgggaaaac aagcgcgtgg tcacccagct tgacgacaca cctgccggtg
    14101 ccgtgctgct gctggaagtc ggcgccacct gcgtaggctc catcgtgcag acttgcgcgc
    14161 ctggcgtcag ggcggccaaa ggcgatgaga aaggctactt ccgcttcggc ggctcctgct
    14221 ttatcaccat cttcgagccg ggccgcgcgc gcttcgccgc cgacctcatg cagcacagcg
    14281 ccgccggccg cgaagtctat gcccgcatgg gcgacgccgc cgccgaggcg ctgacggcgt
    14341 gacgaagctc atccttttat ggaccgcccg ctttcagagt catggcaccc cagatacgtt
    14401 ccttcggcgc ggcctgtctc tcattgtgcc gaggccttcc gatgcgcgct gcatgcccgt
    14461 ttctacattt ccgtttagaa tggcaggaat tttgcctgat cctgggcatt ctaactcgtt
    14521 gattttgaga gtggaggcga gggcgggaat tgaacccgcg cataccggtt ttgcagacca
    14581 gtgcattacc actttgctac ctcgccatcg cctttcgggc gtgagggcat cctagtgagt
    14641 tgccagcgtg tgtcaacatg gcagacggtt cggatggatg aagggcatcc gccccctgac
    14701 ggtccggacc gcgctgtctt gaaaagacct cccaagagtg tttcatgccc gcgcatgagc
    14761 catgatgagc aatccacgat cccgacgagt ccgcctgacg gctcgcggcc cgcgctgacc
    14821 ggcgtgtggc aggcgcctgg tgtggagcag atgcgggcga tgctgccgca gtatgaggtg
    14881 ctggagatga tcggtcgtgg agggatgggg gcggtttaca aaggcaggca gaaatcgctc
    14941 aacaggctgg tcgcgatcaa aatcctgccc ccgggcgcgg cggatgacga gttcaagttc
    15001 accgagcgtt ttcaaaacga ggcgcggacg atggcggcca tgaaccatcc ggccattgtg
    15061 ggcgtttacg actttggcga gacagcggac gggctgctgt atttcatcat ggagttcgtg
    15121 gatggcacgg atgtgcagag gatgatccag accagcggac ggctgagcgg cgagcaggca
    15181 ttgcgcatca ccgccgatgt ctgtgatgcg ctggcctacg cgcacaagcg cggcgtgatt
    15241 catcgcgaca tcaagccggc caacatcctc attgacacgg agggacgggt aaaggtggcg
    15301 gactttggcc tcgccaaaat gcaggatgcc tcgcgcacca gcgcgctgac ccgctccaac
    15361 gtggcgatgg gcacgcccga ttacgtggcg ccggaggttc tcagccccgg aatgacggcg
    15421 gatcatcgcg ccgaccttta tgccgtgggc gtgatgcttt accagatgct caccggcgag
    15481 gtgccgcgcg gcatgttcaa gctgccctcc gagcggacac ccggcgctga tccgcgcttt
    15541 gacgaggtca tctgcaaagc catggagcag gaccgcgagg agcgctacca gaccgcctcc
    15601 gacgtgcgcc aggccctcga atccatccgc accacgccgc tggcgaaaga tcagaacagc
    15661 ggtgccagag cacagcgctc gcgcgcgatg ccgaaggcgc cgtccgcgtc cgtttccctt
    15721 ggcagtttca aatggcgcgc ggcagcggtg ggcgtcgtgg cactgggagc cgccgcattt
    15781 tttgtggtga aaggcggcaa ggagccgcct gcgaaagcgc agcctccgtc gagcgctcag
    15841 gcggacgcaa ctgctcctgt cgctgtgcca gaccgtacga ttgactggat caactcgccg
    15901 ccggccacag tgagcggctg gtcgccggaa tggaacctga gagacggcac gctgacaccc
    15961 agcgcgcaac tgcgctggca attcgggctc atgaaaaacg gccttgtccg tctgcatgtt
    16021 cggctggcgc ctgattacac agagatggac ggaaatggtc cgcagctgca gctcacgatc
    16081 cgcagcggca gcctcaacgg gccgcaagga aggaccgggc acatgcaatt tcagacctat
    16141 gttccaaacc gcacagcctc tcttcatttc caggacaaga cgtcaccggg cctgccggtt
    16201 gagcaggtgg tcgaaggcaa gccattgcag gtgtccgagc ttggctggcg tgaggtccaa
    16261 tgggagttcc gggtcgtggg tgatgagtat tccgcgtggg ccgacggcaa aaagatcgcc
    16321 tcagtgcgtg atgcgcggat tgcctcaggc tactgcatgc tcgaagtccg gcccggaatt
    16381 cagatcacct tgctggaaac cgagggcatc gaaccggtca aagccaccgt tcccgcccct
    16441 gccgtgaagg agacctggac cgaccagctt gccgcccgcg aatggagttc cccctggata
    16501 ttcgagggcg aggtgcttcg caccacggac aagaatccca ccgcgtatct cggacggatg
    16561 accgatggag cgttgcggat cggtttccag ccggagcctg aaaaactgct gcctggcaac
    16621 aacccgccgc tgcaaatctc actccgctcc acttactatg atgaggaggc aaaggtccgc
    16681 ggcatctatc aaattcagat ttatccagac cgccggatca gccacctgct ttatcttgcc
    16741 aaaggtcctc caggccagaa cgacccgccg ccggagtacc tgtggaagga caggccttgg
    16801 cctgatgccg gagcggtcgc ggatttggaa atccgtgcgg agggaggaaa cctggccctg
    16861 cttcaggatg ggaaaaccat cgcatccgca caggacagcc ggctgtctgc cgggtcctgc
    16921 gtcatgatgg ccagtcccgg agtgcgcatt ctcaaactgc agacactggg ggtcgccaag
    16981 gaaaatgagt ccgtgcggag tgacatggcg gcgcctttgc ctcctgacat taggcactgg
    17041 caggatgtga ccgggaccgt gcgggagaaa gcaaagggca tgtccgaatt gcttgtcgag
    17101 gcggatgcgg tggcgcactc cacccgcggg tctccggtga tgatgccttt gtctcccggc
    17161 agttcccgca gccttgctgt ccgcctgacg catgccggcg atggccaggt caattggcgt
    17221 tcgccttcgg ggggcatcct ctatgtgctc tgccagcgca cacagaccat catccagcgt
    17281 cacgccaagg atgcggctgc gccggtgaag atggtccccg atgtgcccca tcccgccagc
    17341 cacagtcatg caaagccgca cgaactgctt gtcactttgg aagggccgct gctaagggcc
    17401 tggcttgacg gccggttcat ctgcgaagtg cgggatgatg ccttcaccgg aggctcctgc
    17461 gaaatcgtga tctccaagtg gggcgatgtg cggaaggtgg agacagctga gcttcaccca
    17521 cccgccagcg tgtggacgga ctggctcggg ccgaaactgg cggcgggcgg ttttgccggc
    17581 gggcagtgga agcgcgaagc cggcggaatc tcgaccgatc aagtcctcgc gggttttgat
    17641 gtcctcccca aagacacgcg cgacggcgcg gtgaaggtga cctttgtgat gcgtggctca
    17701 gaaggcgtgc ttctcgactg ccgggagacg aaggaaagtg gcggccggca cctctacaat
    17761 gcccagtaca ccggcaaaaa cctctatatc tcccactcgc cgccggacgg gagcgcgacg
    17821 aggcgtctga tcaacgagcc ggtcgcggaa gaaatccgtt caagagagga acacactctg
    17881 gagtttcatt tcatcggcga ggagctgact gccatcctcg acggcgtcca ccgcgtctcc
    17941 acccgccaca gcctgctcaa gcaaggtctt tgcgccgtcg tgctcaccca aggcgtgctg
    18001 gtcaaaaaag tggagattca ggcgctggat tctcccaggt agggcggctg gtcgtccagc
    18061 cgccgcgagc gacgctgagg gtgcgaaagt cactgccggt aaatttggac ccgcactctg
    18121 cccaagcggc gcgttgagga caacgcgccc tacccggctt gcgacacggc ttggaagacc
    18181 catctccaac aggcatccct tcctcaccgc cccatcaccg ccatcaggtg ggccagctcg
    18241 gctttttgcg cggcggggtc gctgacggtc tgggcgatct cctcctcaat gagcttgccg
    18301 tagcgctttc tcatgcggtg gatttgcagg cgcagggtgg cctcgctgag gcacagcttc
    18361 tttgcggcct gcgcataggg ggtggcgtgg tcgcccaggg gcaggaattc gcggagctgc
    18421 gcgtagccgt cgaggttgtc cgccttggcg aagtctgcgc gcagcttttc ctccgctgcg
    18481 gaaagcacgc cctcggccca ggcgcggtcg aagatggagt cggggtcgcg gatgtccgct
    18541 ggctctcggg cgtagcggtc ttcggcatcc aattcgtcga gcgacagcgg cgccaggccc
    18601 gcgccgcgtt tttcggcgcg gtcacgccgg agcttttttt ggaagacgtt cttggcggcg
    18661 ccgagcatga aggttctcat gcggcctttg tcctcccgcg cggcgcggat ggaatcatgg
    18721 gtgacgaggc tggcgaagac ctcctgggtg aggtcctcgg cgtcctcgat ggaatgcccg
    18781 gcccggcggg cgaaggcata aatggggtac cagtaacccc ggcaaatctc ctccatggct
    18841 gcggcggcgt ccttttcgcc gccctcccgc actttgcgga tgagcgtcca gcttgtggtg
    18901 gggaagttcg ggttttgggc cactcgcagt gaaactggga ttttttctct ccgcctgaaa
    18961 caaccggact gccgtcggta aagcaagcct gaaccctctg tgtttcgcat gaatcctcat
    19021 cttctccttc ctttcctcgc cgcatccttg tttcttggga gcggcggact ggcggctgac
    19081 ccaggcacgg gcaggatcgt ttatcagcag ttcacggatg cttttgtgaa cgacctcgac
    19141 gggcgcggtg tggagctggt gctcgtggat gccggcggtg gaaaccgtga ggtgatcgcc
    19201 ggttcggagt ttgagaatta cggccatgcc gctctaaacg cggatggaac ccgcgtggcc
    19261 tttgtggcgg agcagggcgg gggcggaccg aagctctggg tgatgatggc ggagccgcat
    19321 gatccgcagg acaatccggc ggtgatggtg cgtgagaatg tcagcgcgca aagccgtgtc
    19381 tgctggtcac ccgatggtcg gcgcatcgcg ttcgcggatg cctttgaccc ccagatttat
    19441 gcgcttgagg cggtggatgg cagtggggat gtcacgcccg aaagcggtga caacctggcc
    19501 gtgcaggtga ccagcctgaa cgcacaggcg caaaaccccg cctggagtcc tgacgggaaa
    19561 ttcctcgcct actcgaatgg cacggcgctt tgggcgctgg ccgtccagga tggctctgga
    19621 gcgctcacgc ccgaggcgcc ggggacccat gaacccgtgc cgctgacttc gaatctctat
    19681 tccggcgagg attttcccgc ctggagtccg gacgggacgc aacttgtgtt ctcctccttg
    19741 cagtcaggtg cgccgggagg tgacaggacg gacatcgcca tccgcacagt gcggcagcct
    19801 cagggggtgg gaggggccat tttgccggcg gctcaggacg gctcggatga cgggcggctg
    19861 tcgctctttc gtgacctgtc cggcggtccc aatcccgact atgtccacga ggcgccgaat
    19921 tttgtgggcg agccttcatg gagtccggac ggcagccgtg tgacctacgg ccaccggttt
    19981 gccggacttc cggagttgaa cacggttatt tcagtcaaac cggagctgga agatgccgtg
    20041 ataaaccccg gtgtggcgct gacggagatc accaacttcg gcacgggcac acccggaggt
    20101 cggcgagccg ttttccagcc tgccgcgccg accggcggcg gggtgctaac cgctcccgaa
    20161 aattttgtcg tctcacccgg ggaggccggc cagccctggt cattcacggc cactctcgac
    20221 tccgatgccc ccggcctttt tctgcgcatt caaagcacgc tgacgccgga cattgaggga
    20281 agctggattg acctgccggg aggcgggcag atgacgcgcg atggcttttt ttggtcgttg
    20341 gaaactgccg tcatccccgc gggaacccgc tatttccgtg tcatcgccgc agccaccggc
    20401 ttgcaggaca gcaagacggt tgaaccgcag agctatgatg tcgtttccac cgccgggcgc
    20461 attctctaca cgcgcggcag cgttcttgac cgcgagctgg tgctcatgga ggcggatggc
    20521 agcggcaaga cggtgctggt cagcggaggg atattcaacg tgcagcagtg cgcgctgagc
    20581 gcggacggcg gtcatgcggc cttcacaaca ctggctggca aactttttgt gatgaaggcg
    20641 gagccgatgg acagcgagac gaatgaaccc gtggacatcc tggaggcggc ggcagtgggg
    20701 ccgatgtttg gctcaggcat cacttggtcg cccttcggcc cgcatctggc ttttgcgggg
    20761 acggacagcc gcgtccatgt gatcgaggtg cttgatgacg gtggcaatat caacccgttc
    20821 aatggctcct ccgtgcccct ggtggatgtg ggcggcacgc tctccggcgc gccgaacccg
    20881 gcttggagtc ccgatggcag gcatctggcg ctgctgggtg caaactccat cgagctgctg
    20941 gaagtggcgg acgtggcagg caacatcacc accggcggcg ccaccaacat gccggtgccg
    21001 ctcacgctgc aaagcgactt tacctccacc aagacacacg ccgcctggtc gcccgatggc
    21061 cagaccatcg cctttgtgga gcgcgccatc agcggtgatc aggcgaaaat ttccctgctc
    21121 accgcctttg atgctttcgg cacgctgacg cccgagatcg ctccaacgca ggagaacgct
    21181 ggcaatccac gccaggaact gcacgccatg acgggcgctc cccaggtgaa aacggtgtcc
    21241 tggagccccg atggcgccct gcttgcattg gagacggaga acaacgggga gcatcgtgtg
    21301 gagatcatcc ggccagcgga gctggacggc tccaacccgc gcatcccttt gaccagccaa
    21361 gggacggacg gcgacgcttt tcaaccatcc ttccagcagc ccgtgagcga cagggcgctg
    21421 cccgcgattg tcgccttcca ggctccgacc tacgcgggca atgaggatgg cgatccaatc
    21481 atcgtccggg tgatgcgcac cggaagtgtg tcgggagaac tggaggtgga cttcacagtc
    21541 accgggggca cggccgaaga cgggctggac tacactctga cagaaagtcc tctcgtcttt
    21601 gccgacgggc agacggaggc gcagatcctc gtcacaccgc tgcctgactc cctggatgac
    21661 gagggggacg agacgatcga attggaactg gtctccacca gttccggagc gctcgggggc
    21721 atcacgagca ccctggtgac gctcaacgac agcgacagca atcaactgcc tttcacccct
    21781 gcggctgcgg agatcgtggt ggtggtgaac ggcaagacgc tcaaaaaagg cggcgccaag
    21841 acaggcgacc gctttgcatt cacagtgcgg caggatctgg gagcgctgct gccgggactc
    21901 gatgtgaaaa tccagacaag cgccactccg aatgaccctg ccagttggac caacgtgcca
    21961 aatctgataa tgaaaagagc caccaagacc agcatgacat ggaagggctc cgtgaccgcc
    22021 tcggtgctgc cgcatggcac tctgcatttc cgcacgcgct cgtatgccac cggtcatcgc
    22081 tccaatgccg gccccgtggc cggtccattc aaaatcaccc ccgcgcccat gctcgtactg
    22141 accacttggg tggttacgga ctcccatccc aacggactca ccgtgaagcc gggcgagttc
    22201 atcacctacc atctgcgctg cgacaacatc ggcgcggcgc cggcaaaaaa agtggtgctc
    22261 aacagtctca ttccggcgca gacaaggctg gactccgcca gccatgacaa cgcggcctct
    22321 ccagggtatt tcaagcgtgt gaccagctcc aagggagtga tcacggacgt gcagtggaac
    22381 gtcggagaca tccaggcagg ccagcatgtc agcgagtttg tcaccgtcca ggtgaatcag
    22441 gaggtggagt tcgccaggct cgttctgaac aaccggctga cttacaaggg aaccggtgtg
    22501 aaggaatttt tcacgccctc gttggccacc cagattgaat cgcccctgct tctgacgctg
    22561 acccgagaca aaaacatcgt ccaggctggc gatctcatca cctacaccct caccgccaca
    22621 aatgacgcct cttatcagat cactggcggc caggtgagaa accagatccc ggatggcact
    22681 cggctttaca gtgtcagaca gggggacggc gcgggcaatt acctcggcac ggagcttgcc
    22741 cacactttgc tggaggagtc gccgcagggc atgacgcgcc ccggctacac gctccacaac
    22801 cgcaccctgc tttggaacat cggcaccctg gccggcggca gccagcgcca gctccgttac
    22861 acggtgcgcg tggcctatga cctgtttgaa aaaatcgccc gcaacggcga gtcctacaac
    22921 acggagatcc gcaacctgaa ctttgacttc actggcacgc cgcccagcgg cggcgtcatc
    22981 gcggcttaca acggcgccat ccccagcgac cgcatcaccc gcaatcccgt ctccgccgag
    23041 actcccgcgc agcggccgga gctgggcctg gtgaaaaagg ccgtctccga caccgtggcg
    23101 acggtgggag gaaaggaggc ggccgccgtc ctcgccgacg agtggcggcg cgtcacctac
    23161 aagattgagg cgtggaacta cggcaacatc accgccgagg acacggtcat ctacgatgtg
    23221 ctgccccagg gcacagactt tgacatggac atgagcctca ctggcagcca tgccgaggac
    23281 atcctcacca ccgatgagcc gcacggcttc accgccggcg agaaaatcat cttcaccgcc
    23341 ctgaccggcg gcaaggggct ggcggtgaac aagatctatt tcgtcagctc ggtgccctcg
    23401 gcggacccgt tcaccgtctc cacctccgcc ggcggaccgg ttctgaactt cactaccgat
    23461 gtcagcgtgg gcgacgcgcg ccgcatcgct tacgactggc cgcgcttcat gtcgcgcttc
    23521 accctggatg gcgcgccctt tgcatttccc gacggcttca ccttcttcga tgaaaaaggc
    23581 gcggtgatcc tgcccggcgg cgaggccttt gtggacacca acggcaacgg caaagtggac
    23641 aaaggcgagt tcgtggacgg caacggcaat ggcaaatacg acgggccgga caaggtgcgc
    23701 agctttgaat tccgcctcgg gcatctgacg cagatgacca ggcccgcagt ccgcaccctg
    23761 agctaccgag tcaaggtgcg cccgacggtg aagtctggca cccagatcac cgccttcggc
    23821 tcctcctcgg tgaaaagagg cgaggggctc gaactcgtca gccccgattt catctacccc
    23881 ctgtccggca cccccgagct gctgcacacc cgtgtcgttt cccggatgac ctggctgatg
    23941 gagccgcccc atcccctgga tgcgggctat gtgacggatg atccgctttt gcgggcggtt
    24001 ccgcatctca tccgcttcag aaacaccagc agcgaccttt acgcgctcga cgccaagctg
    24061 aggattcccg tgccccaggg cttcgaggtc accgcgccgg gcggcagctt gcaaaagttc
    24121 gacgccaagc tagacgccaa gctagacaaa tcccccggca cctttgtcct ggacatcggc
    24181 gaggtggccc ccggccagca gatcgagcgc cagatcacca gccgtttcct gccgcccatg
    24241 cccgcgggct ggacggataa aaaaggcgtc ctgctgcaga cccaggcgtt caaccatgcg
    24301 aacgtcgtct ccacccgcgt cagcagcgag ccgccatcct ccggcttcag cctgctttcc
    24361 accgccgctt ccgagccgag gttcgagatc aagtttgacg cgcgtgccgc gaatgtctcc
    24421 gctttgcctg tcatgaagac ccataccgac ccgcgtgtct tcgtcggccg catcgccccg
    24481 gcggccgtgc aggcggggag tgaaatggac atcttcattt tttacggcaa tcacggcgga
    24541 gtccgcacgg acaatggcga gattgccatg cagatcccct ggggcacctc cctggtggcc
    24601 gccgaaccca tctgctacaa tccagaggct ggaagcccca accaaactca gggtttctcc
    24661 agaggctggt tcaccatcaa gacggaaaaa gggccgaaac gccgtcctca ggatggcaat
    24721 gccggcgtcg tgtttttcaa gtttggcaat atggcgcctg ccgaggtcgg cgtggtgcgg
    24781 gtgcggctct tcgtcacgca ggatttcccc atgggcttcc tcacggacca gtcgctttac
    24841 atcaagttcg acggcgtggc tggaaaatcg ccgccgccgc tctccgtgca ggtgctgcgc
    24901 aacaccagtg gcgccaccga ctggtggcag gcagcgggca atttcttcga caagctgggg
    24961 agcgacatca atgcggccct gcgcaatatc ttcggcggtg gcaacggcga gtttcgttat
    25021 gaccgcgcca ttgtcaccct gggaggcgcg gactttgttc atctcgccag cggtcccatc
    25081 atcattccgc tgggaagcag ccgttccgca atcatcgccg aatatgatga catcgtgggt
    25141 cggatgccat catccgccat catgctgcat ggcgagacag gggtcaggaa cggctacttg
    25201 gcgatggccg ccggcctgac cagcgcccag caagtcttcg ttcctggaag catccagcag
    25261 gaccgcacgg tccatagtat ccttgcggag ctgaccctgc catccagcat tgtcgcggca
    25321 ggcggtggca acatcgtcgc cgctggaggc ggcaatatcg tggcaggagg cggcggcaac
    25381 atcgtcgcgg gaggaggagg caatgtggtg gccaatgacg gcgccggctt cctgcccggc
    25441 agcatcaact tttccattcc gggagtgaat ccaggcatcg tcgcggcggg tggcggcaac
    25501 atcgtggctg caggaggcgg caacatcgtc gcggcaggcg gcggcaacat cgtggccgcc
    25561 gggggaggta acatcgtctc ggcgggcggc ggcaacctca tcagcgttga cggcctcaag
    25621 tttgatatct ctaaaatgag cgcgctgatc gccaagattg cgctgataga ccccgccaag
    25681 ctgctggatg ccggcaatct caaactcatc ggtcaggatg gcgcgggcct catcggccaa
    25741 gacggagcgg gcctcatcgg acaggatggc gcgggtctca tcggtcagga cggagcgggc
    25801 ttcaactttt gatggcccgc ggcgacctta gttgtgacct tcccacattc gcgccacgcg
    25861 ctcgatggcg agggcacggc ctgtggcggg gtcgagtgtg acgagcacgc cgttcacgcg
    25921 cacggttccg cgtcccacgc cgtagcgggc ggggagctgg gtgtggaatt tctccagcac
    25981 gggctcgatc tggctgccga tgaccgactc cagcgggccg cacatgccgg catcgctctg
    26041 gtaggcggtg ccccggggca gcacgcgctc gtcggcggtg ggcacatggg tgtgcgtgcc
    26101 gatgacggcg gtgactttgc cgtcgaggtg ccagcccatg gccacttttt cactggtggc
    26161 ctcggcgtgg aagtccacga agatgatggg tgtatcgagc cgcatccgct caacctcgcg
    26221 ggtgatgacg gtgaaggggt tctcgagctg gtcgcgcatg aagacgcggc cctggagatt
    26281 gatgacgccg actttgcagc ccttctttgt ctccaaaacc aggctgccct ggccgggcgc
    26341 gccctcgggg tagttcaacg gccgcagcac acgcggctcc tcggcaagga aggggacgat
    26401 ctccttctga tcccagatgt ggtcgccggt ggtgatgacg gccgccccgg cgcgcatgag
    26461 cccgatggcg atcttcggcg tgatgccgcg gcctccagct gagttctcgc cattgacgat
    26521 aatgaagtcc accgccagct cctgcttgag cacgggcagc agcgccgtca ccgccttgcg
    26581 gcccggctcg ccgaccacgt cgccgaggaa gaggatgcgg aagtgatcgg aggctgtcgg
    26641 ggagccggat tcagtcgctg cggcggcttc ggaaatcatg gcgcacatag taggtgatgg
    26701 gcgcgcgcat caagtctagg atgcgctcat gacacttctt ttccgcgccg cgctcaccgt
    26761 ctgcgtcctg gccggctccc tggccgcgca gacgccggcg ctgccactgt ccgcgccgcc
    26821 ttccccggcg gacgcgccca gcccggaggt gctggccctg cggctttcca aaacgactcc
    26881 gctgggcaag aagcccgact ggtccggcct cgatgcttat cagggcgtct tcacgcgcgc
    26941 ggagttcgag ttggcgtgga gggagattta ctcgacggag agcgggctgc ccgcgccttt
    27001 gaaaatcctg gccgacaccg tggaggtgcc cacgggacag ccgttgacgc cggtcaagca
    27061 catcgccttc cgcccggaag gcgcgccgcg tccgccgcag cccgggcagg tgcgctcgat
    27121 cgtgaagaac ccctggcttg cgcgcacctt gcccatgccg ccgcaggtcg ggcactgggt
    27181 gggcttggtg ccgtccttgg cgccggtgcc ggtacacgcc tcgcacgcga tgctggtcgg
    27241 cacgcggatt tccgccacct tgtcggtgaa tgcctcgttg aggctgatct ccatgttgta
    27301 gcgcaggtcc gcccc
//
LOCUS       DAOMYJ010000046        27293 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Prosthecobacter sp. isolate AMR_MDS_5629
            NODE_632_length_27293_cov_19.261326, whole genome shotgun sequence.
ACCESSION   DAOMYJ010000046 DAOMYJ010000000
VERSION     DAOMYJ010000046.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867850
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prosthecobacter sp. (wastewater metagenome)
  ORGANISM  Prosthecobacter sp.
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Verrucomicrobiaceae; Prosthecobacter.
REFERENCE   1  (bases 1 to 27293)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 27293)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.30
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:34:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,742
            CDSs (total)                      :: 4,684
            Genes (coding)                    :: 4,676
            CDSs (with protein)               :: 4,676
            Genes (RNA)                       :: 58
            tRNAs                             :: 55
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27293
                     /organism="Prosthecobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_632_length_27293_cov_19.261326"
                     /isolate="AMR_MDS_5629"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1965333"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            179..493
                     /locus_tag="PLP58_06930"
     CDS             179..493
                     /locus_tag="PLP58_06930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009961067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HRJ08285.1"
                     /translation="MNIQKMMKQVQQMQGQMQKAQAEMAARSFEASVAGGKITATANG
                     HGEIQGIRIAKEVVDPEDAEMLQELVLTAVREAQKKAREHQAAEMGKMTGGMGLPPGL
                     GF"
     gene            complement(575..1150)
                     /locus_tag="PLP58_06935"
     CDS             complement(575..1150)
                     /locus_tag="PLP58_06935"
                     /inference="COORDINATES: protein motif:HMM:NF025217.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HRJ08286.1"
                     /translation="MATDSFLQEFFRNWKTVGAVAPSSPALATRMMESAQVGQAAHVL
                     ELGPGTGAFTGAILDSMPHNCRYLGIELNGAFVAKLRGAFPGLDFREAGAQEFDFGSH
                     LEGKQPFDVIVSGLPWTAFPPDLQRAILGNVLPHLKPGGRFATFAYYGFHQMPGGRRF
                     RGLLHELLPGVESSRVVWRNLPPAFVYVGRK"
     gene            1218..1442
                     /locus_tag="PLP58_06940"
     CDS             1218..1442
                     /locus_tag="PLP58_06940"
                     /inference="COORDINATES: protein motif:HMM:NF017785.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YgdI/YgdR family lipoprotein"
                     /protein_id="HRJ08287.1"
                     /translation="MPALTRILTLLPILALCSCQAGPTYKITLKDGREYLCQGVPQFQ
                     KKTGYYRYRTLQDRDAMLRADEIFAIEEQG"
     gene            1442..2014
                     /locus_tag="PLP58_06945"
     CDS             1442..2014
                     /locus_tag="PLP58_06945"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Maf family protein"
                     /protein_id="HRJ08288.1"
                     /translation="MRPLVLASASPRRVQLLREAGFAFEAVSPDGVEEAHDAALTPEA
                     LTVENARRKACAVAALRPEALVIGADTLVYVDGIPLGKPAGHEEARAMLRSLSGRAHE
                     VCTGVFLAWDGGRRGLGFHDVTEVVFKTLADEDIRAYHALVNPLDKAGAYGIQEHGGR
                     IIAGHEGSWTNVMGLPMEKLAAALRDIKAR"
     gene            complement(2048..3535)
                     /locus_tag="PLP58_06950"
     CDS             complement(2048..3535)
                     /locus_tag="PLP58_06950"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase"
                     /protein_id="HRJ08289.1"
                     /translation="MRRALTLSLLSIPLLSLASEKPSILLILPDQMRASAMGCDGNAE
                     VKTPHIDRLAAEGLRFKRTYANVPVCCPARAILMTGTYPHVNGMMANDLRLREEQVTL
                     AEILRDAGYRTGFIGKWHLDGGPREPGFVPPGPRRQGFEFWAAYECHHKHFQPDYFRD
                     TPERIVVDKFEPEASCDFAVEFLKSQPRDKPFFLTVQMGPPHDPYGAPDEYMKQYDAA
                     KLTPDASWQPGSESRPAPKAGLRRGPLANRFVPVGGREEIAAYYAAITALDDQVGRLL
                     ATLKETGADENTIILFTSDHGDMLGNHGMRRKRKPHDESARVPGILRWPARVPKGQVV
                     DTLFSHIDMPPTLLALAGHPVPEHMQGADLSRVALGQTTEGPEAVLLQIFVPFNPDGI
                     ATPWRGIITKDHTYARHENAPWVLFDDKADPHQMRNLAADPAAQELRGKLDAQLAALM
                     KKHGDAWSFNSSETVEEGGRLYKHATFFTVREYLDWAKANPDKAR"
     gene            complement(3603..5126)
                     /locus_tag="PLP58_06955"
     CDS             complement(3603..5126)
                     /locus_tag="PLP58_06955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ08290.1"
                     /translation="MKRRRRASPGKKGRWMPVAAGLAGALVVLVMLAPALLTGWVRAQ
                     LKKDGFRARMEALFGGRLDAEVTLAPLRWTGDEVTVAEAQARLANGWRAEMNGLHLGL
                     DWAAFRQKTWRVIGAGADSLDLVFDPAQVRPGSASAEMSGDDGGKGPSIPAWLRSWLP
                     DKTEVDGARVERFSMRHPAGWQVAGAHLKFSPWLQGDGSLQLTAESGVITTPLTLETL
                     REPVKLRLDRAALRLAPAEAHLKEADLKWLDTAITARGRMRLEDKSWSLSANFERLPL
                     AEVLAEDWRLRLTGHLTGEVELAASPGAQPRAKGRVTLKEGALTALPVLDRLAAYTNV
                     ERFKRLVLDVAETSLEGTAGAQVFDKIVLQSNGLMRLEGRLAVEGEKLDGSFLLGVTP
                     ETLRWMPGTQKHVFTLPNPNGPDGMVWTTLRITGTRSAPREDLSSRILQGAGRAVLDA
                     PGEVTAQAGELLLTPVLGKDAATAPGTVIKGATEAGGKAVESGVRLLEGIGGGLLKP"
     gene            complement(5178..6494)
                     /locus_tag="PLP58_06960"
     CDS             complement(5178..6494)
                     /locus_tag="PLP58_06960"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013646.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HRJ08291.1"
                     /translation="MPDPQVIIIGAGPAGCTLAALLAMRGVECLVFDDDKRPGLLVGE
                     SLIPGVVPVFQRLGIEERVAAISVRKPGASFFAGPDGPRIHFKFKNVEGHLPPYSFNT
                     PRPELDNLLRRRAEELGARFVHARAELVACERGGQSSVRLSSRSRAQAGLSTDAEPLL
                     VDASGRARVFSRLMGLGAKKGGRDDVAHFAHFEDFEHDEVEPGQVIISVLKAGWSWRI
                     PLPGRLSVGIVVGRERAKELGATPEERLERAIAQEPLLREKGARARRVTPVMTYTNYQ
                     LLSERGHGPGWVLLGDAFGFVDPMLSPGLFMSLEGARLLDERVFARGADFLDKPEKLA
                     RALDGYAREVVRWHGAWMELVEYFYNGRIFQMHLAGRDMALKPAPFDLASRMERHTTF
                     HLASMASGGMTRSAYSRGLIRFLSKHLVWGVPAAANFAVRDACAPA"
     gene            6584..7423
                     /gene="folP"
                     /locus_tag="PLP58_06965"
     CDS             6584..7423
                     /gene="folP"
                     /locus_tag="PLP58_06965"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009961179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004156 - dihydropteroate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HRJ08292.1"
                     /translation="MNHLWRAGSRTLDLSRRGLVMGIVNVTPDSFSDGGRFADTGRAV
                     EHALRLLDEGADILDIGGESTRPGADPVPEAEEIRRVLPVIQALRTQTSALISIDTMK
                     AAVARAALDAGAGIINDVTGLRGDPLMPRLAAECRAGLVVMHMTGTPRTMQARAAYTD
                     VVAEVTGYFHERLALLEGMGISPERVVLDPGFGFGKTLEHNLALMQSLPRLSECGRPV
                     LVGVSRKSMIGQLLGSDDLADRDWPTVALTSYAREQGARIVRVHDVRPSVEAMRMTEA
                     ILA"
     gene            7614..7889
                     /locus_tag="PLP58_06970"
     CDS             7614..7889
                     /locus_tag="PLP58_06970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ08293.1"
                     /translation="MDNPSDTRAETERNFGPQPLDALMLQLGLDNHALVAASQEPLTH
                     KAVQRARKGRRLTPRMRQRIADALHRAATLKDARPERPWYPRELFNY"
     gene            complement(7948..9198)
                     /gene="purD"
                     /locus_tag="PLP58_06975"
     CDS             complement(7948..9198)
                     /gene="purD"
                     /locus_tag="PLP58_06975"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009960575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004637 - phosphoribosylamine-glycine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HRJ08294.1"
                     /translation="MKILVTGKGGREHALITALSESPGGHELFAWPGSDAISALAARV
                     DAPDLPGLISWMKTNGIDLCVAGEEAWLVKDGGLANLCEQAGIPCWGPFKEAARLESS
                     KIFAKRFLQRHGIPTAAFTTASTADEARAALTGFPIVLKFDGLAAGKGVAVCAGRAQA
                     EEFIADVLERRVFGPGDLLIEQCLSGPEISIFASVCDGRHQILMPARDYKRIRDNDEG
                     PNTGGMGAVAARALADPALMERIEREIVRPTVDGLQKDGLRYRGFLYFGIMLTPQGPQ
                     VLEYNCRFGDPEAEAVLPMLRGDFAAYVDAAARGALNPGLINFAEGWSICIISASAGY
                     PASSRCGDVIRGLDTVSGARVFHCGTRLNPDGLYETNGGRVLAVVAKGATREEARAKA
                     AAESAKITFDGLQRRSDIGLLHFT"
     gene            9314..10006
                     /locus_tag="PLP58_06980"
     CDS             9314..10006
                     /locus_tag="PLP58_06980"
                     /inference="COORDINATES: protein motif:HMM:NF016206.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metallopeptidase"
                     /protein_id="HRJ08295.1"
                     /translation="MLGIFAIVFLLAFLVCRHALGRYEQVLRQGAREAAPTAHTGAEI
                     ARLFFASEGITDVEIVEHEGMTSNCFDPRRRRLFLTRETAGGATLAAWAVALHEAGHA
                     AQTGEALGDVKWRQTVIRLTRYGPGFAVILAGALMLMKFNARMAVFACAGLCLAFLLL
                     NLGTLAVEFNANARLRRFLEKHLERHHDALERLGGHLSKVATREVGDLLRSPRFFLLS
                     GLPGSGKIRPKS"
     gene            10225..10560
                     /locus_tag="PLP58_06985"
     CDS             10225..10560
                     /locus_tag="PLP58_06985"
                     /inference="COORDINATES: protein motif:HMM:NF012314.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HRJ08296.1"
                     /translation="MSKPLSASSSAGGSAVMSTVMASKVPVLVEFHAEWCGPCRAVGP
                     VVEELARELSGSGKARVVRIDVDKDPQAAAAHGVRGIPAFIVFQNGRETARQTGAIPK
                     ALMRQMLGL"
     gene            complement(10567..12678)
                     /locus_tag="PLP58_06990"
     CDS             complement(10567..12678)
                     /locus_tag="PLP58_06990"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009958438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family metallopeptidase"
                     /protein_id="HRJ08297.1"
                     /translation="MDTPFLTQDFHIRWSTLAPAAIQADITRALREAEQRLNAVSEQD
                     RGKMSFESVILALEEATRPLNEAWGLVQHLDALCNSPELREAHNAMLPEVSAFFAKIP
                     LNEHLWDLLETYAKTEEARSLPPVRARALDETMEGFRQAGADLPPEKKKRLEELESEL
                     SQATQKYSENVLDSTNAWELVLPDTGRLKGLPASAIEAARADAAAKGLGTPERPQYRL
                     TLKAPSMIPVMEHAEDETLRRQVWEGSVSIGRGAHDNTDLIWKILRLRQEKAELLGKP
                     HFADLVLQQRMAKNGTAALAFIEGLHAKVKTAFDRETMQLQEFRAEHSGKRADLLEPW
                     EVAYWAERQRKAAYDFDEEELRPYFPLDKVLGGMFRLAELVFDLRIVGRDVVCVKPGD
                     KIEASTRPEALGPVEVWHPEVKFYEMRNEKGVHIGSFYADWHPRDSKRGGAWMNYLKG
                     GSPPRGDLDRRLHLGLICGNMTPPVEGRPALLTHDEVCTVFHEFGHLLHQLLGNVEVP
                     SLNGVSVYWDFVELPSQIMENFCWERESLDLFARHHETGEPIPQRLLKKVLAARNYRS
                     ASDIMRQLSFGKLDLELHMRRAFEEGADLDDLTRRLLKDYLMPLKTENPTMARRFGHL
                     FSSPVGYAAGYYSYKWAEVLDADAFTRFQQEGVLNPKVGREFRDKILSRGNSEDPAKL
                     FRDFMGRDPDPTALLARAGLA"
     gene            12798..13149
                     /gene="ssrA"
                     /locus_tag="PLP58_06995"
     tmRNA           12798..13149
                     /gene="ssrA"
                     /locus_tag="PLP58_06995"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            complement(13284..13895)
                     /locus_tag="PLP58_07000"
     CDS             complement(13284..13895)
                     /locus_tag="PLP58_07000"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009962007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HRJ08298.1"
                     /translation="MPSLDHENQLRAAGHLRVCGIDEAGRGPLAGPVCVAGVILPEGF
                     AHPVLNDSKQLRENQREQLHTELMADARLRWHCEIVSAAEIDEINILQATWLGMRRCA
                     RALQADAALIDGKPVRDFPVPQRALVKGDSLSYSIAAASIIAKVTRDRLMRALAEKHT
                     GYGFDIHKGYPTPAHLAALKRLGPCPEHRRSFAPVAACFELGL"
     gene            13982..15232
                     /gene="fabF"
                     /locus_tag="PLP58_07005"
     CDS             13982..15232
                     /gene="fabF"
                     /locus_tag="PLP58_07005"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007418887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HRJ08299.1"
                     /translation="MSERRVVITGIGVVSPIGNNKDDFWKNLVAGKSGIRRIQSMDTT
                     NYDCKIAGEVVDFDPTPFFNNHKEARRADRFFQMAMAASKMAVKDSGLNPDSLDLHRI
                     GVMVGSGIGGLSTIETQYEILLNKGPGRVSPFLIPMMITNIATGMIATEFGFMGPNMC
                     ITTACATSNNNIGEAWRIIKFGDADAIVCGGSEASIRPCGLSGFANMKALSLRNDEPE
                     RASRPWDKDRDGFVMGEGSGVVVIEELEHAKKRGATIYCELAGYGVTADAYHLTAPHP
                     DGIGASKCMEMALRHAKLNAEEVQYVNAHATSTPVGDLCELRAIKRTFGSFAQKGLLV
                     SGTKSMTGHLLGAAGGIELAASVLALRDQIVPPTINVENPDPEVDVDIVANEARPAKL
                     TAALSNSFGFGGHNSSVLVKKFEG"
     gene            15322..16152
                     /locus_tag="PLP58_07010"
     CDS             15322..16152
                     /locus_tag="PLP58_07010"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015411.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HRJ08300.1"
                     /translation="MSESLDIGLLGQGIIGSRVAENLRKAGHCVHVWSRTGRAGGLPS
                     PRVVAQKAEILQIFTRDSEALLAALNDARPALTARHVVMNHATVSKAATLEAARICAE
                     AGAAFLDAPFTGSKMAAQNAKLVYYIGGDEAVLERARPVLELSSAKILPVGKIGDAMV
                     LKIVTNLVTAVIVKALAEAAAITQAQGVPLEALRTALEANANYSALIGMKLPALMSGD
                     FEPHFSLVNMLKDVDFARALAAESGLPVPAVDCAAEAMRAGVAEGLGEQDFSIMGRLP
                     "
     gene            16149..17036
                     /locus_tag="PLP58_07015"
     CDS             16149..17036
                     /locus_tag="PLP58_07015"
                     /inference="COORDINATES: protein motif:HMM:NF013720.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ08301.1"
                     /translation="MNAELFQRLETEGGWLDFSRRAKWRLSGADRVRYLNGQVTQDAR
                     RADARSALHACVTNAKGRIEGDIFFHAAPDGESLLLDAPAGLRETLGMRLERYIIADD
                     ALLEDVTEEWQLWHRFGPSVDANGTTGGLICKRLACPGMDVWLPADAPPPGEALSEED
                     AGTLRILRGVPAWPHELNADVFPQEAGLEAETMSYTKGCYIGQEVLSRIKSTGRMPRE
                     LVLWTSEDEVAGGALLWREDGTKAGVVTSAARHPLSGLMQGLAYARQGAVEKDSRLLV
                     GNDMASIAASLSIQTLPCR"
     gene            17033..17317
                     /locus_tag="PLP58_07020"
     CDS             17033..17317
                     /locus_tag="PLP58_07020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ08302.1"
                     /translation="MMPLRLLMTALALLSIVSCEFGGRADVRNPTVEELDRLDVEWGL
                     PQRKSRGAPKRTYQYTEPSPGYSPAAPADAPAAQAPPRETIQGLPPGAPQ"
     gene            17349..18236
                     /locus_tag="PLP58_07025"
     CDS             17349..18236
                     /locus_tag="PLP58_07025"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF012387.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0016018 - cyclosporin A binding [Evidence
                     IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HRJ08303.1"
                     /translation="MKTSPIFFALLAAPALMAQTSAPDQPLAPIPPLTPLPPIERLAD
                     QAQRELFDAAAGSVPKNDGPVVSQSGRKTTFSALPGEDPSAWKSDELRKLAVMEVSFG
                     GAVETVMFVLHPQDAPQTVSNFLDNCETGAYNGLAFHRAIEGFIVQTGDPLTSDEGAR
                     ERWGTGGEEKTVPAEIKRKHRKGAVAMGRRSDSVNPGRRSNGFQFYFALGNFGSLDGK
                     YTVFGQVVSGLETLERISKMPVDSNDCPVARIEIKSLKVLDQKGPLQEPVRDTGDGRK
                     YTKPAAAKGFFERFLERVW"
     gene            18276..18644
                     /locus_tag="PLP58_07030"
     CDS             18276..18644
                     /locus_tag="PLP58_07030"
                     /inference="COORDINATES: protein motif:HMM:NF018508.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YkvA family protein"
                     /protein_id="HRJ08304.1"
                     /translation="MPASDNQHDIDIAKVSERAQDIEDKLPKLRQWMAQAKLMLALVR
                     DYWSGRYRQVPYWVISACALALLYVLNPLDVIPDVIPGLGYLDDATVVAFCLKLIERE
                     LERYRLWKEGGGGGQAMTNA"
     gene            complement(18654..19541)
                     /locus_tag="PLP58_07035"
     CDS             complement(18654..19541)
                     /locus_tag="PLP58_07035"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015679.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_function: GO:0071972 - peptidoglycan L,D-transpeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HRJ08305.1"
                     /translation="MPPSLVLPRTCQTALAGLVICLAACKTAPPPPPPEPEPVKKPAG
                     LYEWKGQGKNITSIKINVDEQKARLYHGEDEIGWTYVASGITSFPTPVGEFKILEKVA
                     DKVSNLYGKGYDANGKLVNSDFKQGRDLLPPGGRFEAAKMTYFMRLTGDGVGMHIGPI
                     PRPGRRASHGCIRLPSGIAGTMFRNTSVGTPVTITGSGPDYKTYLKQSAEKAKANAAK
                     LAASKKKAEEEAAKALAEGSTPPAEPPAANGAPPSTVTTALPADAPVPAPLVPATPAP
                     PPAPSAPIEEIRKVDVPEE"
     gene            complement(19575..20234)
                     /locus_tag="PLP58_07040"
     CDS             complement(19575..20234)
                     /locus_tag="PLP58_07040"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HRJ08306.1"
                     /translation="MLKAALFDLDGTLIDSLADIAGAINEMLDGRGWPRCEVELFREM
                     VGDGMDRLVERALPPHARTPELIQTCVEEYRANYDKRWQRETRPYEGITELLAALKAR
                     DIRLGVISNKAHRFTVPMTEHFFGAGCFDIILGQRAEVPRKPDPAAGLEVVQALRLDP
                     KDCAYVGDSGIDMEFARRAGMTGIGVTWGFRSERELRENGAHILVARPLEILKCLESA
                     G"
     gene            complement(20274..21299)
                     /locus_tag="PLP58_07045"
     CDS             complement(20274..21299)
                     /locus_tag="PLP58_07045"
                     /inference="COORDINATES: protein motif:HMM:NF012546.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HRJ08307.1"
                     /translation="MFPRVFILSLLMLPTSAFTQLPSAWPDGVGEILVKSTADGTEQP
                     ALAWSPRTAEARPLLVGLHTWGGDYKQTSNGPVFARWCMERGWHFVFPHFRGPNRTPE
                     AMGGNLAVQDIADAVAWMKKTRRVDEARVYLIGASGGGHMAMLMAGRHPEIWAGVSAW
                     VGISDIAAWHAEHLKDGMPDNYAKNIESALGGPPDSEQRLADARRRSPLTWLHQARGV
                     NLDISHGIHDGRSGSVAFRHSLHAFNAVVPEADRLPLAEIAAYYETMQRPANWPAPEP
                     DPVFEPKSVLFRKTSGNTRVTLFEGGHEILKTPGLNWLAAQQRGKPAVWEIKEVFKIQ
                     GGTEAGR"
     gene            complement(21352..22803)
                     /locus_tag="PLP58_07050"
     CDS             complement(21352..22803)
                     /locus_tag="PLP58_07050"
                     /inference="COORDINATES: protein motif:HMM:NF024491.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase D-like domain-containing protein"
                     /protein_id="HRJ08308.1"
                     /translation="MSFSPTLALTWEMIVLGAVALAFSLTALLHLLTHHRDHRSAGFW
                     VALILFSPVVGACLYALLGINFVRRKGRDYREAIGVVYRQPAPDCPLFIHGEPESAVN
                     DCALATTLDRISRFHFASGNRVEILENGDEAMPAMLEAIRAACSSVSLSSYIFEAHGI
                     GSEFVRELVSAGARGVEVRVIVDDAGTRYSWPPVTRELRRLGVPVRRFMPNRLVMRLI
                     TLNLRNHKKLLIVDGAAAFTGGMNIRQGNMILQNPPHPVRDLHFRVTGPVVEQMQRVF
                     AEDWQFCAGEPLEGNAWFPKLQPAGATHALGIVDGPDEDIEVMPAALFASLNAARSRV
                     CIITPYFLPLSHLMAALKLCSLRGVEVMIVTPARNNIPLVAWAARTLYPELVKTGCRI
                     FESPPPFDHSKMLLVDDAWCCLGSTNWDPRSLRLNFEFNLACHDPDLVRRLSAVFEKK
                     RAESVEVTAAMLAETPLRHRLRNGFASLFIPVL"
     gene            complement(22905..23813)
                     /locus_tag="PLP58_07055"
     CDS             complement(22905..23813)
                     /locus_tag="PLP58_07055"
                     /inference="COORDINATES: protein motif:HMM:TIGR02532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HRJ08309.1"
                     /translation="MKRQLQRGFTLIELLVVITIIAIIASLAVPTYNLITVKANQMKG
                     GSNCRQIIGLLMTYAADNNGLYPDSVPSPVTGSVPLTANDAFRALIIEGLTQDESIFG
                     CPGSIFNPNKNIGIPPTFDQALMAGENHWAMTQAQSNTSSSIMPIVFENPAAPSWPPQ
                     WNCDAAGKPVPGRAWQGGTVLVGKNDASVETVKLMAAQGASVPPRMLGTGFDIFTMAS
                     QNQPQQILNVVAGGGGSYTTMQGGVPGQFPGAPGGLPQLPAAGGLPGQMPGGLPQLPG
                     AAPGGLPQLPGAAPAPAPAPVPAPQQ"
     gene            complement(23999..25186)
                     /locus_tag="PLP58_07060"
     CDS             complement(23999..25186)
                     /locus_tag="PLP58_07060"
                     /inference="COORDINATES: protein motif:HMM:NF015418.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin molybdotransferase MoeA"
                     /protein_id="HRJ08310.1"
                     /translation="MISEAEALRRILDAVTPLPVAEVPLPDALGLSCARTMLADFPLP
                     GFDNSMMDGYALRAAESTGERILNLKGVQPAGLDQSLICGPGEAVRVFTGAPIPAGAD
                     AVIMQEDVQVLEDAIRCLEPVAPGENVRRAGADLCAGQIMLKAGDALTPGRLGLLASQ
                     GHATVPVHAPPKAALLTTGDELRPPGAALLPGQIYNSNGVMLRAMLAQAGVRGATAAH
                     CEDDFNATLEKLKRLIREHDMILVSGGVSVGARDQVKPALGALGIEPEIWRVKVKPGK
                     PFLFAQTTWQGRAVHIFGLPGNPVSGFVTFQLFVRPALLRLLGRTDTEPESLPAVCGR
                     NLSNDSDRPHYLRGQLRHDRFIPQGVQMSHALFGLSQADTLLRLEPGQTIHEGENVRV
                     FPF"
     gene            complement(25183..25680)
                     /locus_tag="PLP58_07065"
     CDS             complement(25183..25680)
                     /locus_tag="PLP58_07065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ08311.1"
                     /translation="MPALPPIPVHALACILLAGCSALKNKPPKENKDDDSTVLVGIVE
                     MVNPEQEYVLIRCEQMPAFSPGTELVAVSALGERSRLVLTPERKGWYITADIREGRPE
                     VTHLVIHERAGAAGKLTPAPATPLIQPAMPPGQQQPSTLSLPSLPGLPEGFETSASPL
                     PSSEP"
     gene            complement(25738..>27293)
                     /locus_tag="PLP58_07070"
     CDS             complement(25738..>27293)
                     /locus_tag="PLP58_07070"
                     /inference="COORDINATES: protein motif:HMM:NF012356.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HRJ08312.1"
                     /translation="AAGAGDAGRQLEDIADLLEKLPAPQLLDMLQSNDLQVLMDAYAD
                     RSLSTTSEPLGVIVERERARFSAWYEFFPRGAEGRYDRHSTFRDCLPRLDDAKTMGFD
                     VIYFPPIHPIGITARKGKNNTLTAHADDVGSPWAIGGPAGGHREIEPKLGTLDDFVWL
                     LEEAKKRGLEIALDFAINCSPDHPYVREHPEWFYQRPDGSIRYAENPPKKYQDIYPLN
                     FHCADWKNLWRELIDVVLFWVDKGVKIFRVDNPHTKPVSFWEELIKTVHRKDPEVIFL
                     AEAFTKPKMMQVLGKIGFSQSYTYFTWREDKAALAEYARELTQGEMRWYYRGNFWPNT
                     PDINPFHLQNAPASMFRMRAALAALLSSSWGMYAGYELCENEPLPGREEYLDSEKFQL
                     RQRDYNAPGNIKGFIGRLNRIRQEHRALQLYDNLVFHDADHNAMLCFSKCAPDFSDRI
                     LCVISLDGHQPVAGQARLNLAALGLDPARPYRAHDLMHDQAYDWQGAENYVELHPNGT
                     ALHVFAITQ"
BASE COUNT         4699 a         8657 c         9078 g         4859 t
ORIGIN      
        1 ggaacccgag gccgcgcccc cgcccgccga cgaaagcggg agcgccgccg ctccgggcga
       61 aggcgcggag tccgcgccac cggctgatga ctttcacaac gacccgctga tccgcgccgc
      121 catcgaaacc ttcaaactca aactcgccgc gcgctgacgc ggcgccccat ttacccccat
      181 gaacattcag aagatgatga agcaggtcca gcagatgcag ggccagatgc agaaagcgca
      241 ggccgagatg gccgcccgca gctttgaagc cagcgtcgcc ggcggcaaaa tcacggccac
      301 cgccaacggc cacggtgaaa tccagggcat ccgcatcgcc aaagaggtgg tggaccccga
      361 ggatgctgag atgctccagg aactcgtgct caccgccgtg cgcgaggcgc agaagaaggc
      421 cagggagcat caggccgccg agatgggcaa aatgacaggc ggcatgggcc tgccacccgg
      481 cctgggcttt tgaggccgac gcggaggccg cgcttttgca tgggtgacag gcttccccgc
      541 ctgtccgcgc ggacaggatg cgcgcgcccc atgctcactt ccgccccaca tagacaaagg
      601 ccggcggcag attccgccag accacgcggc tggactcgac gccgggcagc agctcgtgca
      661 gcaggccgcg gaagcggcgt ccgccgggca tctgatggaa gccgtaatag gcgaaggtgg
      721 cgaagcggcc gcccggcttc aaatgcggca gcacattgcc caggatggcg cgctgcaagt
      781 ccggcgggaa agccgtccac ggcaggccgc tgacgatgac atcgaagggc tgctttccct
      841 ccaagtggga gccgaagtca aactcctgcg cgccagcctc ccggaaatcg agtcctggga
      901 aagcgccgcg cagcttcgcc acaaaagcgc cgttcagctc gatgccgagg tagcggcagt
      961 tgtgcggcat ggagtcgaga atcgcgccgg tgaaggcgcc cgtgcccggc cccagctcca
     1021 gcacatgcgc ggcctggccg acctgcgcgc tctccatcat gcgcgtggcc agggccgggc
     1081 tggagggggc cacggcgccg acggtcttcc agttgcggaa gaattcttgg agaaaggagt
     1141 cggtggccat gcgcgccgat ggtaggcgag ttgacggcgc gcgcgaacgt ttttcattgt
     1201 ccgccatcca agcactcatg ccagccctca cgcgcatcct caccctgctg ccgatcctcg
     1261 ccctctgctc ctgccaggcc gggccgacgt ataaaatcac gctcaaggac gggcgcgagt
     1321 atctctgcca gggcgtgccg cagttccaga aaaagacggg ctactaccgc taccggacat
     1381 tgcaggaccg cgacgcgatg ctgcgcgcgg atgaaatctt cgccatcgag gagcagggct
     1441 gatgcggccg ctcgtcctcg catccgcctc gccccgccgc gtccagcttc tgcgggaggc
     1501 gggctttgca ttcgaagccg tctctcccga cggagtcgag gaggcgcatg acgccgcgct
     1561 gacacccgag gcgctgacgg tggagaacgc ccgccgcaag gcatgcgccg tcgccgccct
     1621 ccgcccggag gcgctcgtca taggcgcgga cacgctggtt tatgtggatg gcatcccgct
     1681 cggcaagccc gccggtcatg aagaggcgcg cgccatgctg cgcagcctct caggccgcgc
     1741 gcacgaggtc tgcaccggag tctttctggc ctgggacggc gggcgccggg gcctcggctt
     1801 tcatgacgtg acggaggtcg ttttcaaaac cctcgccgac gaagacatcc gcgcctatca
     1861 cgccctggtg aatccgctcg acaaagccgg cgcctacggc atccaggagc acggcggcag
     1921 gatcatcgcc ggccatgaag gatcatggac gaacgtcatg ggcctgccga tggaaaagct
     1981 ggccgcggcg ctgcgggaca tcaaagcgcg ctgacttcaa ggagccgggc ctgccaagtc
     2041 cggcaggtca acgcgccttg tccggattcg ccttcgccca gtcgaggtat tcccgcacgg
     2101 tgaagaaggt ggcgtgtttg tagaggcggc cgccttcctc cacggtctcg ctggaattga
     2161 aactccatgc atcaccgtgc ttcttcatca gcgcggcgag ctgcgcgtcg agttttccgc
     2221 gcagttcctg cgcggcggga tcggcggcga ggttcctcat ctgatgcggg tccgccttgt
     2281 catcaaagag cacccagggc gcgttttcat gccgcgcata ggtgtggtct ttcgtgatga
     2341 tgccgcgcca gggtgtggcg atgccgtcgg ggttgaaagg cacaaaaatt tgcagcagca
     2401 cggcctccgg cccctccgtg gtctggccga gcgcgacgcg cgaaaggtcc gcgccctgca
     2461 tgtgctccgg cacgggatgc cctgccaagg cgagcaacgt gggcggcatg tcaatgtggc
     2521 tgaacagcgt gtccacgacc tgtcccttcg gcacgcgcgc gggccagcgc aggatgccgg
     2581 gcacgcgggc ggactcgtcg tgcggcttgc gcttccgcct catgccgtgg ttgcccagca
     2641 tgtcgccgtg gtcggaggtg aaaaggatga tcgtgttctc atccgcgccg gtttccttga
     2701 gcgtggcgag caggcggccc acttgatcat cgagcgcggt gatcgcggcg tagtaggcgg
     2761 cgatttcctc cctgccgccg acgggaacga agcggttcgc cagcggtcca cggcgcagtc
     2821 cggcctttgg agcgggccgg ctttcgctgc cgggctgcca gctcgcatcg ggcgtgagtt
     2881 ttgccgcgtc gtactgcttc atgtattcat caggcgcgcc atacgggtca tgcggcggcc
     2941 ccatctgcac ggtgaggaag aagggcttgt ccctgggctg cgatttgagg aactcgaccg
     3001 cgaaatcgca ggaggcctcg ggttcgaact tgtccaccac gatgcgctcc ggcgtgtcgc
     3061 ggaagtagtc gggctggaag tgcttgtgat ggcactcgta ggctgcccag aactcgaatc
     3121 cctgccggcg cggccccggc ggcacaaagc ccggctcgcg cggcccgccg tcgagatgcc
     3181 atttgccgat gaagccggtg cggtagcccg cgtcgcgcag gatttccgcg agggtgacct
     3241 gctcctcgcg cagacgaagg tcattggcca tcatgccgtt cacatgcggg taagtgccgg
     3301 tcatcaaaat ggcacgcgct gggcagcaca cgggcacgtt cgcgtaagtg cgcttgaaac
     3361 gaaggccctc ggcggccaga cggtcaatgt gcggcgtttt cacctccgca ttgccatcgc
     3421 agcccatggc gctggcgcgc atttggtcag gcaggatgag gaggatgctc ggtttttcag
     3481 acgccaggga aagcagtggg atggagagaa gggaaagtgt gagcgcgcgc cgcatggaaa
     3541 tggaaacggc gggcagtgcg gcgtgtttcc gcccatgagt gtccggtttt ttcaactccg
     3601 gctcaaggtt tgagcaaacc gccgccgatg ccttcgagca aacgaactcc cgactccacc
     3661 gccttgccgc cggcctcggt cgcgcccttg atcaccgtgc ctggggcggt ggcggcgtct
     3721 ttgccaagca cgggtgtgag cagcagctcg cccgcctgcg cggtcacttc ccccggagcg
     3781 tcgagcacgg cccgtcccgc gccttgcagg atgcgcgagc tgaggtcctc gcgcggcgcg
     3841 gagcgggtgc cggtgatgcg cagcgtggtc cagaccatgc cgtccgggcc gttcgggttc
     3901 ggcagggtga agacatgctt ttgcgtgccc ggcatccagc gcagcgtctc cggtgtgacg
     3961 ccgagcagga agctgccgtc gagcttttcc ccctccacgg ccaggcggcc ttcgaggcgc
     4021 atgagtccgt tggattgcag cacgatcttg tcgaagacct gcgcgcccgc cgtgccttcc
     4081 agggaggtct cggccacgtc gagcaccagc cttttgaagc gctccacgtt ggtgtaggcg
     4141 gccagcctgt cgagcaccgg cagcgcggtg agcgcgccct ccttgagcgt cacccgccct
     4201 ttggcgcgcg gctgcgcgcc ggggctggcg gcaagctcca cctcgcctgt cagatgcccg
     4261 gtcagccgca gccgccagtc ctccgccagc acctccgcca gcggcagccg ctcgaagttc
     4321 gccgacaggc tccacgactt gtcctccaag cgcatccgtc cgcgcgcggt gatcgcggtg
     4381 tcgagccatt tcaaatccgc ctccttgaga tgcgcctccg ccggtgccag ccgcagcgcc
     4441 gcgcggtcga gcctcagctt cacaggctcg cgcagcgtct ccagtgtcag cggcgtggtg
     4501 atcacgccgc tctcggcggt gagctgcagc gagccgtccc cctgcagcca ggggctgaat
     4561 ttcaaatgcg cgccggccac ctgccagcca gccggatgcc gcatggaaaa gcgctccacc
     4621 cgcgcgccgt ccacctcggt cttgtctggc agccacgacc gcagccaggc gggtatggac
     4681 ggccccttgc cgccgtcgtc cccgctcatt tccgcgctcg cggatcccgg cctgacctgc
     4741 gccgggtcaa agaccaggtc cagactgtcc gcgcccgcgc caatgacgcg ccatgtcttc
     4801 tgccggaacg ccgcccagtc cagtcccagg tggaggccgt tcatctccgc gcgccatcca
     4861 ttcgccagcc gtgcctgggc ctcggcgacg gtgacctcat cacccgtcca gcgcagcgga
     4921 gccagtgtga cctcggcgtc cagcctgccg ccaaagagcg cctccatccg cgcgcggaag
     4981 ccgtcctttt tgagctgcgc gcgcacccag cctgtgagca gggcgggggc cagcatgacc
     5041 agcacgacca gcgcccccgc cagaccggcg gcgacgggca tccaacgacc tttctttccg
     5101 gggctggcgc ggcggcggcg tttcatgccg gcaattaaaa accccgcccc gcttcgcgca
     5161 acgccaaagc cggggcatca cgccggcgcg caggcgtcgc gcacggcgaa gtttgccgcc
     5221 gccggaactc cccacaccag atgcttcgaa aggaaacgga tcagcccccg gctgtatgcc
     5281 gagcgcgtca tgccgcccga tgccatgctg gccaggtgga acgtggtgtg ccgctccatg
     5341 cgcgaggcca ggtcaaaggg cgcgggcttc agcgccatgt ccctgcccgc caggtgcatc
     5401 tggaaaatgc gcccgttgta aaaatactcc accagctcca tccacgcgcc gtgccagcgt
     5461 accacctcgc gcgcgtagcc gtccagcgcg cgcgccagtt tttccggctt gtccagaaag
     5521 tccgccccgc gcgcaaacac gcgctcatcg agcaggcgcg cgccttccag tgacatgaag
     5581 agccccggcg aaagcatcgg gtccacaaac ccgaacgcat ctcccagcag cacccagccg
     5641 ggtccgtggc cgcgctcgga gaggagctga tagttcgtgt aagtcatcac gggcgtcacc
     5701 cgccgcgcgc gcgcgccctt ttcccgcagc agcggctcct gcgcgatggc gcgctccagt
     5761 ctctcctccg gcgtggcgcc cagctccttc gcgcgctccc tgcccaccac aatgcccacc
     5821 gagagccgcc ccggcagcgg aatgcgccag ctccagcccg ccttgagcac cgagatgatg
     5881 acctgccccg gctccacctc gtcgtgctcg aagtcctcga aatgggcgaa gtgcgccaca
     5941 tcatcccgcc cgcctttttt cgcccccaga cccatcaggc gggaaaatac ccgcgccctg
     6001 cccgatgcat ccaccagcag cggctccgcg tccgtggaga gcccggcctg cgcgcggctg
     6061 cgtgatgaca gacgcactga agactgcccg ccgcgctcgc acgccaccag ctcagcgcgc
     6121 gcatggacga agcgggcgcc cagctcctcg gcgcgccggc gcagcagatt gtccagctcc
     6181 gggcgcggag tgttgaagga gtagggcggc aggtgcccct cgacattctt gaacttgaaa
     6241 tggatgcgcg gcccgtccgg cccggcgaag aaggaagcgc ccggcttgcg caccgagatc
     6301 gccgccacgc gctcctcgat gcccagcctc tggaacacgg gcaccacgcc ggggatcaaa
     6361 gactcgccga ccagcaggcc cggccttttg tcgtcatcga agaccaggca ctccacgccg
     6421 cgcatggcca gcagcgcggc cagtgtgcag ccggcaggtc cggcgccaat gatgatgact
     6481 tggggatcgg gcatttccat gaaatggatc aacgtccgcc aaaggcaagc cgccattctc
     6541 tttgccgcct ttactttccc cgccgcatgg gttgaactgc cgcatgaacc acctctggcg
     6601 cgccggctcc cgcacactcg atctgtcacg gcgcggcctc gtcatgggca ttgtcaacgt
     6661 cacgcccgac tccttttccg acggcggccg ctttgccgac accggccgcg ccgtggagca
     6721 cgcgctgcgc ctgctggatg agggcgctga catcctcgac attggcggcg agtccacccg
     6781 gccgggcgcc gaccccgtgc ccgaggcgga ggagatccgc cgcgtgctgc ccgtcatcca
     6841 ggccttgcgc acgcagacct ccgcgctcat ctccattgac acgatgaagg ccgccgtggc
     6901 ccgcgccgct ctcgatgccg gggccggcat catcaacgac gtcaccggcc tgcgcggtga
     6961 tcccctcatg ccgcgcctcg ccgcggaatg ccgtgccggc ctcgtcgtca tgcacatgac
     7021 cggcacaccg cgcaccatgc aggcgcgggc cgcctacacc gatgtcgtgg ccgaggtcac
     7081 gggctacttc catgagcggc tcgcgctgct tgagggcatg ggcatctccc cggagcgcgt
     7141 cgtgctcgat ccgggcttcg gctttggaaa aactctggag cacaacctcg cgctcatgca
     7201 gtccctgcca aggctctccg aatgcgggcg gcccgtcctc gtcggcgtct cgcgcaaatc
     7261 catgatcgga caactgctcg gcagcgatga cctcgccgac cgcgactggc ccaccgtcgc
     7321 cctgacctcg tatgcgcgcg agcagggcgc gcgcatcgtc cgcgtccacg acgtccgccc
     7381 cagcgtcgaa gccatgcgca tgaccgaggc catcctggca tagcctttgc cgcgccggcg
     7441 cggcagcgat aaagtcccgc aatcgccggc cagacaagcg tgccggaagg ccggcggagt
     7501 ttgaaggccg cagtctggac ggcgaagtca ctgtcattcc attcgcctga tccggtttgc
     7561 ggaagatgaa gacaggaacc cggaccggga ttaggatggc ggtcagaact ttcatggaca
     7621 atccctccga cacccgcgcc gagactgagc gcaatttcgg cccgcagccg ctggacgcgc
     7681 tcatgctcca gctcggcctg gacaatcacg ccctggtcgc cgccagccag gagccgctca
     7741 cgcacaaggc cgtgcagcgt gcgcgcaaag gccgccggct gacgccgcgg atgcgccagc
     7801 gcatcgccga cgcgctccac cgcgctgcca cgctcaagga cgcccggccc gaacgcccgt
     7861 ggtatccgag ggagcttttc aattattgac ggcgcgcatc ccgtgcgaac aaaaaccgcg
     7921 ccctgcgaat cgccggggaa tgcgaagtca ggtgaagtgc agcaggccga tgtcggagcg
     7981 gcgctggagg ccgtcgaagg tgattttggc gctttcggcg gcggccttgg cgcgggcttc
     8041 ctcgcgtgtg gcgcccttgg ccacgacggc gagcacgcgc ccgccatttg tctcgtagag
     8101 gccgtccggg ttcaggcgcg tgccgcagtg gaagacgcgc gcgccgctca ccgtgtccag
     8161 cccgcggatg acatccccgc agcgggagct ggcggggtag ccggcggagg cggagatgat
     8221 gcagatgctc cagccttcgg cgaagttgat gaggcctgga ttcagcgcgc cgcgcgcggc
     8281 ggcgtccaca taagcggcga agtccccgcg cagcatcggc agcacggctt cggcctcagg
     8341 gtcgccgaaa cggcagttgt actccagcac ctgcggcccc tgcggagtga gcatgatgcc
     8401 gaagtaaagg aagccgcggt agcgcagtcc gtccttttgc agtccgtcca cggtcgggcg
     8461 cacgatctcg cgctcgatgc gctccatgag cgcgggatca gcgagcgcgc gggcggccac
     8521 ggcgcccatg ccgccggtgt tgggcccctc gtcgttgtcg cggatgcgct tgtagtcgcg
     8581 cgcgggcatg aggatttgat gccgcccgtc gcacacggag gcgaagatgc tgatctcggg
     8641 accggagagg cattgctcga tgagcagatc gcccgggccg aagacgcggc gctccagcac
     8701 gtcggcgatg aattcctccg cttgcgcgcg gccggcgcag acagccactc ctttgccggc
     8761 ggcgaggccg tcgaacttca aaacgatggg gaacccggtc agcgcggcgc gcgcctcatc
     8821 cgcggtggag gcggtggtga aggcggcagt cgggatgccg tgacgctgga ggaagcgctt
     8881 ggcgaatatt ttcgaggatt caagccgcgc cgcctccttg aacggtcccc agcaggggat
     8941 gccggcctgc tcgcaaaggt tggccaggcc gccatccttg accagccacg cctcctcgcc
     9001 ggcgacgcag aggtctatgc cgttcgtttt catccatgag atgaggccgg gcaggtccgg
     9061 cgcgtccacg cgcgccgcca gggcggagat ggcatcgctg ccgggccagg cgaagagttc
     9121 gtgcccgccg ggggattcgc tgagcgccgt gatgagggcg tgctcgcgcc cgcctttgcc
     9181 tgtgaccaga attttcatgg ggcgtgatgg atggagcggg aacgcgggat ggcaagcgct
     9241 ggcagcggtt ggagggggag tttgaatctc gaatttcgaa tttcgaaatt cgttttgcac
     9301 gcttcaatgc gccatgctcg gcatcttcgc catcgtcttc ctgctcgcct ttctcgtgtg
     9361 ccgccacgcg ctgggccgct atgagcaggt gctcaggcag ggcgcgcgcg aggccgcgcc
     9421 gacggcgcac acgggcgcgg agattgcgcg gctgtttttc gcctcggagg gaatcactga
     9481 tgtggagatc gtcgagcatg agggcatgac ctcgaactgc ttcgaccctc gccgccgccg
     9541 gctctttctc acgcgcgaaa ccgccggcgg cgccactctg gccgcctggg cggtggcgct
     9601 gcacgaggcg ggacacgccg cccagactgg agaagcgctc ggagatgtga agtggcggca
     9661 gacggtgatc cgcctcactc gttacggccc cggcttcgcc gtgatcctgg ccggggcgct
     9721 gatgctcatg aagttcaacg cgcgcatggc ggtctttgcc tgcgccggcc tgtgcctggc
     9781 gtttctgctg ctgaacctcg gcaccctggc ggtggagttc aacgccaacg cccgcctgcg
     9841 ccgctttctg gaaaaacatc tcgagcgcca tcacgacgcg ctcgaacggc tcggcggtca
     9901 tctttccaaa gtcgccacgc gcgaggtggg cgatctgctg cgctcgccgc gcttctttct
     9961 cctcagcggc ctgcccggca gcggcaaaat ccgcccgaag tcgtgacgcg cgcgaaggtg
    10021 gcagggattt tgtgattcgc tcctgctgag aaccgctcgg atcacaaagt tcctccagag
    10081 tccgcgcagg gcagccgggg ggcggattga ttttccactt ggcagccggt gaatgtgacg
    10141 ggagcgtacc atgtgaaagc cgccaatctc ggtctctggc tcaccggcgc cgtccttgtc
    10201 tgggcgggct ggatattttt ggacatgagc aaacctctct ccgcctcatc atccgctggc
    10261 ggctcggcgg tcatgtccac tgtcatggct tcgaaagtgc ccgtgctggt cgaattccac
    10321 gccgaatggt gcggcccctg ccgcgcggtg ggaccggtgg tcgaggagct ggcgcgcgag
    10381 ttgtctggtt ccggcaaggc cagggtggtg cgcattgacg tggacaagga tccgcaggct
    10441 gcggcggccc acggcgtgcg cggcataccg gccttcatcg tgtttcaaaa cggacgcgag
    10501 accgcccgcc agaccggtgc catcccgaag gcgctcatga ggcagatgct cggcctctga
    10561 ggtccctcac gccagcccgg ctcgcgccag cagagccgtg gggtccggat cgcggcccat
    10621 gaagtcgcgg aacagcttcg ccgggtcctc cgagttgccg cggctgagga ttttgtcacg
    10681 gaactcgcgc cccaccttcg ggttgagcac gccctcctgc tgaaagcggg tgaaggcgtc
    10741 ggcatccagc acctcggccc acttgtagct gtagtagccg gcggcgtatc ccaccgggct
    10801 gctgaacaag tgcccgaagc gccgcgccat tgtcggattc tccgttttca agggcatcag
    10861 atagtccttg agcaggcggc gtgtgagatc atcaaggtcc gcgccctcct cgaacgcgcg
    10921 gcgcatgtgc agttccaggt ccagcttgcc gaaggaaagc tggcgcatga tgtcgctggc
    10981 gctgcggtag ttcctggcgg ccaggacttt cttgagcagg cgctgcggga tcggctcgcc
    11041 cgtctcatgg tggcgcgcga acaaatccag gctctcgcgc tcccagcaga aattctccat
    11101 gatctgggag ggcagttcga cgaagtccca gtaaacgctg accccgttga gcgagggcac
    11161 ctccacattg ccgagcagtt ggtgcagcag gtggccgaac tcatgaaaca ccgtgcacac
    11221 ctcgtcatgg gtcagcagag ccggacgccc ttccaccggc ggcgtcatgt ttccgcagat
    11281 caggcccagg tgcaggcgcc ggtctagatc cccgcggggt ggggagccgc ctttgaggta
    11341 gttcatccac gccccgccgc gcttcgagtc gcgcggatgc cagtccgcgt aaaaggagcc
    11401 gatgtggacg cccttctcgt tgcgcatctc gtaaaacttc acctcaggat gccacacttc
    11461 cacggggccc agcgcctcgg ggcgcgtgct tgcctcaatt ttgtctcccg gcttcacaca
    11521 gaccacatcc cgccccacga tgcgcaggtc gaaaaccagc tcggccagcc ggaacatgcc
    11581 gccgagcacc ttgtccaggg ggaaataagg ccgcagttct tcctcgtcga aatcgtacgc
    11641 cgccttgcgc tgcctctccg cccagtaggc cacctcccaa ggctccagca aatccgctcg
    11701 cttccccgag tgctcggccc ggaattcctg gagttgcatt gtctcccggt cgaaggcggt
    11761 tttgaccttt gcatgcagtc cctcaatgaa tgccagggca gccgttccgt tttttgccat
    11821 gcgctgctgg agcacgaggt cggcaaagtg cggcttgccc agaagttccg ccttctcctg
    11881 ccgcaggcgc aggattttcc agatcaggtc cgtgttgtca tgcgcgccgc ggccgatgct
    11941 caccgacccc tcccacacct gccggcgcag ggtctcgtcc tccgcatgct ccatcaccgg
    12001 aatcatcgag ggcgccttca aggtgaggcg gtactgcggt cgctccggag tgcccagccc
    12061 ctttgccgcc gcgtctgcgc gcgcggcttc gatggcggag gcgggcagtc ccttcaggcg
    12121 gccagtgtcc ggcagcacca gttcccaggc gttggtcgag tcgagcacgt tttccgaata
    12181 cttctgcgtc gcctgggata gctccgactc cagttcctcc agcctttttt tcttctccgg
    12241 cggcaggtcc gcgccggcct ggcggaagcc ctccatcgtt tcatcgagcg cgcgtgcccg
    12301 cacggggggc agcgaccggg cctcttccgt cttcgcgtag gtttccagca ggtcccacag
    12361 gtgctcgttg agcgggattt tggcaaagaa cgcgctcacc tccggaagca tggcattgtg
    12421 cgcctcgcgc agctcgggcg agttgcagag ggcgtccagg tgctgcacca ggccccaggc
    12481 ctcgttcagc ggacgcgtcg cctcctccag cgccaggatc accgactcga agctcatctt
    12541 accccggtcc tgttcgctga ccgcgttgag cctctgctcc gcctcccgca acgcccgcgt
    12601 gatgtccgcc tggatggccg caggggcgag agtggaccag cggatgtgaa aatcctgggt
    12661 gaggaagggg gtgtccatgt tggggcgcga gttttaaacc gcgctcgttg ccgcgcaaat
    12721 ggaaagctcc ctccgagtcc gacaaacgcc ttgacctccg gccctcatcg gcgagtctgc
    12781 atgcgtcaac actgaccggg ggcgtcctgg tttcgacggg tagtctgcgg tcggggtggc
    12841 atgccgagga tgattcgttg gcctcgttaa tcaccggatc aaaaccataa ctgcaaactc
    12901 taacgagctc gcactcgcgg cctaagcacc gcgacatttc cagggccatg tctggtagcc
    12961 ctggagatgc caccaccagg ctggctggct gaccgggcga ccgggtcgaa agcgagattc
    13021 cacaggacgc tgggtttggg gcattccgcc gtcgaaggcc cccaaccgag atcaaatggc
    13081 aggctaagca tgtagaagct ccggcttatg gccattcgga caggggttca actcccctcg
    13141 cctccacttc cttgattttt caagaaattc ggggtggatg ggttggtttt ggcaacattt
    13201 ttggcaacac atcgcccggc aacgcccgtg tcggcgaggg gcgcggttgg gagtggcaac
    13261 gcacatccgc acccgacttc atttcaaagt cccaactcaa aacatgcggc gacgggggcg
    13321 aagctgcggc ggtgctcggg gcatgggccg agccgcttca aggcggcgag gtgcgcgggc
    13381 gtggggtagc ctttgtggat atcgaagccg tagccggtgt gcttttccgc cagggcgcgc
    13441 atgaggcggt cgcgcgtgac tttggcgatg atgctggcgg cggcgatgga gtagctgagg
    13501 ctgtcgcctt tgaccagtgc gcgctggggc acggggaagt cgcgcacggg tttgccatct
    13561 atcaaggcgg cgtcggcctg aagggcgcgg gcgcagcgcc tcatgccgag ccaggtggcc
    13621 tggaggatgt tgatctcgtc tatctcggcg gcgctgacga tctcgcagtg ccagcgcagg
    13681 cgcgcgtcgg ccatgagctc ggtgtgaagt tgctcgcgct ggttttcgcg gagctgcttg
    13741 gagtcgttca gcacagggtg ggcgaagcct tcgggcagga tgacgccagc cacgcacacg
    13801 gggccggcca gggggccgcg tccggcctcg tctatgccgc agacgcggag gtggccggcg
    13861 gcgcggagct ggttttcgtg gtcgaggctg ggcatttgga cgttgattga aaattccttg
    13921 ccccggcatg taaagcgtgt tctctcaacc tccagttcaa aaccaccccc acaccagcca
    13981 catgagcgaa cgccgcgtag tcatcacagg aatcggagtc gtttccccca tcgggaacaa
    14041 caaggacgat ttctggaaaa acctcgtcgc cggaaagagc ggcatccgcc gcatccagag
    14101 catggacacg acgaactatg actgcaagat cgcgggcgag gtggtggact ttgatccgac
    14161 gcctttcttc aacaaccaca aggaggccag gcgcgcggac cgctttttcc agatggccat
    14221 ggcggcctcg aagatggcgg tgaaggacag cggcctgaac ccggacagcc tggacctgca
    14281 ccgcatcggt gtgatggttg gcagcggcat cggcggactg agcacgatcg agacccaata
    14341 cgagatcctg ctcaacaagg ggccgggccg ggtgtcgccc ttcctgatcc cgatgatgat
    14401 cacgaacatc gccacgggca tgatcgccac cgagttcggc ttcatggggc cgaacatgtg
    14461 catcaccacg gcgtgcgcca cctccaacaa caacatcggc gaggcatggc gcatcatcaa
    14521 atttggcgac gcggacgcca tcgtctgcgg cggctccgag gcgagcatcc gcccgtgcgg
    14581 cctgtccggg tttgcgaaca tgaaggccct gtctttgcgc aatgacgagc cggagcgcgc
    14641 ctcccgcccc tgggacaaag accgcgacgg attcgtgatg ggcgagggct ccggcgtggt
    14701 ggtgatcgag gagctggagc acgcgaagaa gcgcggagcg acgatttact gcgagctggc
    14761 aggctatgga gtgacggcgg acgcctatca cctcacggcg ccgcacccgg acggcatcgg
    14821 cgcctcgaag tgcatggaga tggcgttgcg ccacgcgaag ctgaacgccg aggaggtgca
    14881 gtatgtgaac gctcacgcca cctcgacgcc ggtgggggat ctgtgcgagt tgcgggcgat
    14941 caagcgcacc tttggcagct tcgcgcaaaa gggactgctg gtctccggca ccaagtccat
    15001 gaccggccac ctgctgggcg cggcgggtgg catcgagctc gcggcgagtg tgctcgccct
    15061 gcgcgaccag atcgtgccgc cgacgatcaa cgtggaaaat cccgatcctg aagtggatgt
    15121 ggacatcgtg gccaacgagg cgcgcccggc gaagctgaca gcggcgctga gcaacagctt
    15181 cggcttcggc ggccacaact cctcggtgct ggtgaagaag ttcgagggtt gagccacttc
    15241 ggctgcggct cgtgctggat tcagtcacgg attacgggaa gggctttact gcgatttgat
    15301 gatatgagcg cgacttcaac gatgagcgaa tctttggaca tcggcctgct cgggcagggc
    15361 atcatcggca gccgggtggc ggagaacctg cgcaaggcgg gccattgcgt gcatgtgtgg
    15421 agtcgcacgg gcagggcagg gggcctgcca agcccgcgcg tggtggcgca gaaggcggag
    15481 atcctgcaaa ttttcacgcg cgactcggag gcgctgctgg cggcgctcaa tgacgcgcgt
    15541 ccggcgctga cggcgcggca tgtggtgatg aaccacgcca cggtgagcaa ggcggcgacg
    15601 ctggaagcgg cgcggatttg cgcggaggcg ggcgcggctt tcctcgacgc gccctttacc
    15661 ggcagcaaga tggcggcgca gaatgccaaa ctggtctatt acatcggagg cgacgaggcg
    15721 gtgctggagc gcgcgcggcc ggtgctggag ctgagttcgg cgaagatttt gccagtgggt
    15781 aaaatcggtg acgcgatggt gctcaagatc gtgaccaacc tggtgacggc ggtgatcgtg
    15841 aaagcgctgg ccgaggcggc ggcgatcacg caggcgcagg gcgtgccgct ggaggccctg
    15901 cggactgcgc tggaggcgaa cgcgaactac tccgcgctca tcggcatgaa gctgcccgcg
    15961 ctgatgagcg gggacttcga accgcacttc tcgctggtga acatgctcaa ggatgtggat
    16021 ttcgcccgcg cgctggcggc ggagtcggga ctgccggtgc cagccgtgga ctgcgcggcg
    16081 gaggcgatgc gcgcgggagt ggccgagggg ttgggtgagc aggatttttc catcatgggc
    16141 cgcctgccat gaacgcggag ctttttcaaa gactggagac ggagggtggc tggctggact
    16201 tttccaggcg ggccaagtgg cggctgtccg gcgcggatcg cgttcgctac ctgaacggcc
    16261 aggtgacgca ggacgcgcgc cgcgcggatg ccaggtccgc tttgcacgcc tgcgtgacga
    16321 acgcgaaggg ccgcatcgag ggcgacatct ttttccatgc cgcgccggat ggcgagagcc
    16381 tgctgctcga cgcgcccgca gggctgcgcg agacgctggg catgaggctg gagcgttaca
    16441 tcattgccga tgacgcgctg ctggaggatg tgacggagga gtggcagctc tggcaccgct
    16501 ttggcccctc agtggatgcg aatggaacga caggcgggct gatctgcaaa agactggctt
    16561 gcccgggcat ggatgtctgg ctgcctgcgg atgccccgcc gcccggcgag gcattgagcg
    16621 aagaagatgc cgggacgctg cgcatcctgc gcggagtgcc ggcatggccg catgaactga
    16681 acgcggacgt ttttccccaa gaggcgggtc ttgaggctga gacgatgagt tacaccaaag
    16741 gctgctacat cggccaggag gtgctctcgc gcatcaagag cacgggtaga atgccgcgcg
    16801 agctggtgct ctggacctcg gaggacgagg tggctggcgg cgcgctgctt tggcgggagg
    16861 atggcacgaa ggcgggcgtg gtgacgagcg ccgcgcggca tccgctgagc gggctcatgc
    16921 aagggctggc ctatgcgagg cagggggcgg tggaaaagga ttcgcggttg cttgtcggaa
    16981 acgacatggc tagtatcgcc gccagtcttt cgatccaaac cctgccctgc cgatgatgcc
    17041 cctccgcttg ttgatgaccg ccctcgcgct tctttccatc gtttcctgcg aattcggagg
    17101 cagggcggat gtcaggaatc caacggttga ggaattggac cggctggatg tggagtgggg
    17161 actgccgcag cgcaaatcgc gcggcgcgcc gaagcgcacc tatcaataca cggagccgtc
    17221 accgggttat tcgccagcgg ctccagcgga cgcacccgcc gcgcaagcgc ctcctcgtga
    17281 aactatccag ggcttgccgc ccggcgctcc gcagtgagga agacgcaaac ccatccgttc
    17341 ctgaagccgt gaaaacctcc ccgatctttt tcgcactgct ggccgcgccc gccctcatgg
    17401 cacaaacttc cgcgccggac cagccgctgg ctcccattcc tccgctcacg cctttgccgc
    17461 cgatcgaaag actggccgac caggcccagc gcgagctgtt tgacgctgcc gctggcagcg
    17521 tgccaaaaaa cgacggcccc gtggtgtccc agagcggtcg caagaccact ttctcagcgc
    17581 ttccggggga ggatccctcc gcttggaagt ccgacgaact gcgcaagctc gccgtcatgg
    17641 aggtgtcctt cggcggcgcg gtcgagacgg tgatgttcgt gctccatccg caggatgctc
    17701 cgcagacggt ctccaacttt ctcgacaatt gcgagaccgg cgcctacaac ggcctggcat
    17761 tccaccgcgc cattgagggc ttcatcgtgc agacaggaga tcctctcacc tccgacgagg
    17821 gcgcgcgcga gcgctggggc acgggcggcg aggaaaaaac cgtgcccgcc gagatcaagc
    17881 gcaaacaccg caagggagcg gtggccatgg ggcggcgaag cgactcggtg aatccaggac
    17941 gccgctcgaa cggatttcag ttttatttcg cgcttggcaa tttcggctcg ctggatggca
    18001 aatacaccgt ctttggccag gtggtgtccg gcctggagac gctggagcgc atttccaaaa
    18061 tgcccgtgga cagcaacgac tgccctgtag cacgcattga gatcaagtcc ctcaaagtgc
    18121 tagaccaaaa aggcccgctt caggagcctg tgcgcgacac gggtgacggc cgcaagtaca
    18181 cgaagccggc ggccgccaag ggattcttcg agcgcttttt ggagcgtgtg tggtaaggcg
    18241 cgggaaccgg cggtgtttta taacccaata ccgccatgcc cgcctccgac aaccagcacg
    18301 acattgacat cgccaaagtc agcgagcgcg cgcaggacat cgaggacaag ctgcccaaac
    18361 tgcgccagtg gatggcacag gcaaagctga tgctcgccct cgtgcgcgac tactggtcgg
    18421 gccgctaccg ccaggtgccc tactgggtga tcagcgcctg cgcgctcgcc ctgctgtatg
    18481 tcctgaaccc gctggatgtg attcctgatg tgatacccgg ccttggttat ctggacgacg
    18541 ccaccgttgt ggcattttgc ctcaagctca tcgagcggga actggagcgc taccggctct
    18601 ggaaggaggg cggcggtggt ggtcaagcca tgacgaatgc ttgacatccg cgctcattcc
    18661 tcgggcacgt ccaccttgcg aatttcctcg atgggagcgc tcggagcggg aggtggtgct
    18721 ggcgtcgcgg gaaccagggg cgcgggcacg ggggcgtccg ccggcagggc cgtcgtcaca
    18781 gtggacggcg gcgcgccatt ggcagcagga ggctcggccg ggggcgttga gccttcggca
    18841 agcgctttgg cagcctcctc ctcggctttc tttttggagg cggcgagctt ggcggcgttg
    18901 gctttggcct tttcggcgga ctgtttgaga taggtcttat agtcggggcc gctgccggtg
    18961 atggtgaccg gagtgcccac tgaagtgttg cgaaacatgg tgccggcgat gccggatggc
    19021 aggcggatgc agccgtggga ggccctgcgc ccgggacgcg ggatggggcc gatgtgcatg
    19081 ccaacaccgt caccggtgag ccgcatgaag taggtcattt tggccgcttc aaaacgcccg
    19141 cctggcggca ggaggtcgcg tccttgtttg aagtcggagt tcaccagctt gccattcgcg
    19201 tcgtaaccct tcccatagag gttcgaaacc ttgtcggcga ccttttcaag gattttgaac
    19261 tcgccgacgg gtgtcgggaa actggtgatg ccggatgcca cataggtcca gccgatctca
    19321 tcctcaccgt ggtaaagccg ggctttctgc tcatccacgt tgattttgat cgaggtgatg
    19381 tttttgccct ggcctttcca ttcatagagg ccggcgggct ttttcacagg ctccggctcc
    19441 gggggaggag gtggaggcgc ggttttacat gcggccaggc agatgacaag accggcaaga
    19501 gcggtctggc aggtgcgtgg caggacaagg gaagggggca ttgtggcgga tgctaatacg
    19561 cttcactgcc agtctcaacc agcggattcc agacatttca aaatttccag aggccgagcg
    19621 accaggatat gcgcgccgtt ttcacgcaat tcccgttctg aacgaaatcc ccaggtgacg
    19681 ccgatgcccg tcatgcccgc gcgcctggcg aactccatgt ctatgccgga gtcgccgaca
    19741 taggcgcagt cctttggatc cagcctcagc gcttgcacca cctccagtcc ggctgccggg
    19801 tccggtttac gcggcacttc ggcgcgctgg ccgagaatga tgtcaaagca gcccgcgcca
    19861 aagaagtgct ccgtcatcgg cacggtgaaa cggtgggctt tgttcgaaat cacgcccagc
    19921 cggatgtcgc gcgctttcaa tgctgcgagc agttccgtga tgccctcata gggccgtgtc
    19981 tcccgctgcc agcgcttgtc atagttggcg cggtattcct cgacacaggt ctgtatcaac
    20041 tccggggtgc gcgcgtgtgg aggcagcgcg cgttcgacga ggcggtccat gccatcgccc
    20101 accatctcgc ggaaaagctc cacctcgcag cgcggccagc cgcgcccgtc gagcatttcg
    20161 ttgatggccc cggcgatgtc tgcaagcgag tcaatgaggg ttccgtcgag gtcaaaaagg
    20221 gcggctttga gcattgccat tcgttgccac ggcgcgccat cgcgcgcaag gcgtcatctg
    20281 ccggcttcgg tgccgccctg gattttgaaa acctccttga tctcccagac ggctggcttg
    20341 ccgcgttgct gcgcggccag ccagttcaag cccggggttt tgagaatctc atgaccgcct
    20401 tcgaacaggg tgacgcgcgt gttgccggag gttttgcgga acagcacgct tttcggttca
    20461 aaaaccgggt ccggttccgg cgctggccag ttggcgggac gctgcatggt ttcataatag
    20521 gcggcgattt ccgccaaggg cagccggtcc gcctccggca ccaccgcgtt aaaggcatgg
    20581 agactgtgcc ggaaggcgac actgcccgaa cgtccatcgt ggatgccgtg ggagatgtcg
    20641 agattcacac cgcgggcttg gtgcagccag gtgagaggac tccggcggcg ggcgtcggca
    20701 agacgctgct cgctgtccgg agggccgccg agggcggatt cgatgttttt agcgtagttg
    20761 tccggcatgc catctttgag atgctcggca tgccaggccg cgatgtcgct gatgcctacc
    20821 caggcggaca cgccggccca gatttctgga tgccgcccgg ccatgagcat ggccatgtga
    20881 ccgccgccgc tggcgccaat gagatagacc cgggcctcgt ccacccggcg cgtctttttc
    20941 atccaggcca cggcgtcggc gatgtcctgc acggcgaggt tgccgcccat cgcttcgggt
    21001 gtgcggttgg gaccgcggaa atgagggaag acaaagtgcc agccgcgctc catgcaccag
    21061 cgggcgaaga ccggcccgtt cgaggtctgc ttgtaatccc caccccaggt gtgcaggccc
    21121 accagcagcg ggcgtgcttc ggctgtccgc ggtgaccagg ccagcgcggg ctgttcagtg
    21181 ccgtccgccg tgcttttcac aaggatttca ccgactccgt ccggccaggc ggagggaagc
    21241 tgggtgaatg cggaggtcgg gagcatgagc agggacagga tgaagacgcg cgggaacatg
    21301 acaagcaaac ggcacggcct tgacggtcat ttcccgcatt gttcgccggg gtcaaaggac
    21361 agggatgaag aggctggcga agccgttgcg cagccggtgg cggaggggtg tttccgcaag
    21421 catggcagcg gtgacctcca cgctttcggc gcgttttttt tcaaagacgg cggacaaacg
    21481 gcggacgagg tccgggtcat gacaggcgag attgaactcg aagttcaggc gcaggctgcg
    21541 ggggtcccag ttggtcgagc cgaggcagca ccaggcgtca tccacaagca gcatcttcga
    21601 gtgatcaaaa ggcggcggcg actcgaaaat gcggcagcct gttttgacca gttcgggata
    21661 aagcgtgcgc gcggcccagg cgacgagcgg gatgttgttg cgcgcgggtg tgacgatcat
    21721 gacctccaca ccgcgcaggg agcaaagctt cagcgcggcc atgaggtggc tcaaaggcag
    21781 aaaatagggg gtgatgatgc agacacgcga gcgcgctgca ttcaggctgg cgaacagcgc
    21841 cgccggcatc acttcgatgt cctcgtccgg accgtccacg attccgagcg catgggtggc
    21901 tcccgctggc tggagttttg gaaaccaggc atttccctcc aatggctctc ccgcgcagaa
    21961 ttgccagtcc tcggcaaaca cgcgctgcat ttgctccacg acggggccgg tgactctgaa
    22021 atgcagatcg cgcaccgggt gcgggggatt ttgcaggatc atgttgccct ggcggatgtt
    22081 catgccgccg gtgaaggcgg cggctccgtc cacgatgagg agctttttgt ggttgcgcag
    22141 gttcagggtg atgagccgca tcacgaggcg gttcggcatg aagcgacgca ccggcactcc
    22201 cagcctgcgc agttcccgcg tgaccggcgg ccaggagtag cgtgtgccgg cgtcatccac
    22261 gatgacgcgc acctccacac cgcgcgcgcc ggcgctcacc agctcgcgga caaactccga
    22321 gcctatgcca tgcgcttcga agatgtagct ggaaaggctg acgctggagc acgcggcgcg
    22381 gatggcctcc agcatggcag gcatggcttc gtccccattt tccaggattt ccaccctgtt
    22441 gcccgaagcg aaatgaaagc gtgagatgcg gtccagcgtc gtcgccagag cgcagtcgtt
    22501 cacggcggat tccggctccc catgaatgaa aagcgggcag tccggagcgg gctggcggta
    22561 aaccacgccg atggcctccc tgtagtcgcg gcctttgcga cggacgaaat tgatgcccag
    22621 aagcgcgtaa aggcaggcac cgaccaccgg tgaaaacagg atgagcgcca cccagaatcc
    22681 cgccgagcgg tggtcgcggt gatgcgtgag caggtgcagg agcgcggtca ggctgaaggc
    22741 gagcgcgacc gcgccaagca cgatcatttc ccaggtcagg gccagtgtgg gagaaaatga
    22801 catgtgctga gcattgatga gcaaggcgga agttcgcgag cgaaaaagaa gacgccgccg
    22861 gagtgaccgg cggcgtccga ggtatcaagg ggcatccagc ctgatcactg ctgcggggca
    22921 ggcaccgggg ctggcgcggg cgccggagcg gcgccaggga gctgcggcag accgccagga
    22981 gcggcgccgg gaagctgagg caggccgccg ggcatctgac cgggcagtcc gcctgctgca
    23041 gggagctggg gcagaccgcc gggagcaccg gggaattgtc cgggcactcc tccttgcatc
    23101 gtggtgtagc tgccgccgcc accggctacg acgttgagga tctgctgggg ttggttctgg
    23161 ctggccatcg tgaagatgtc gaagcctgtg ccaagcatgc gcggaggcac ggaggcgccc
    23221 tgcgcggcca tcagtttcac ggtttccacg ctggcgtcgt ttttgccgac cagcacggtg
    23281 cccccctgcc aggcgcgtcc gggaaccggt ttgccggcgg cgtcgcagtt ccactgcggc
    23341 ggccagctcg gcgcggccgg attctcaaac acgatgggca tgatgctgga gctggtgtta
    23401 gactgcgcct gcgtcatggc ccagtggttt tctccggcca tgagagcctg gtcgaaggtg
    23461 ggcgggatgc cgatgttttt gtttggattg aagatgctgc cggggcagcc aaagatggac
    23521 tcatcctgcg tgaggccctc aatgataagg gcgcggaagg cgtcgttggc ggtgagcggc
    23581 acgctgccgg tgacagggct gggcacggag tccggataga gaccgttgtt gtcagccgcg
    23641 taggtcatca gcaggccgat gatctggcgg cagttggagc cgcctttcat ctggttggcc
    23701 ttcaccgtga tgaggttgta agtggggacc gccaggctcg cgatgatcgc gatgatggtg
    23761 atcaccacca ggagttcgat gagggtgaac cctcgttgca gttgtcgttt catgttcgtg
    23821 gtgtttctat gttttggatt atgatgtatg cgccgcctgg aattatttcc ctgcggcaaa
    23881 tgcattagac ctgacaggcc tgcaaaaggc gagaaaaaat ttgcgctctg aatgagtcgt
    23941 cactcccgcg atgcctgaaa actcgtccca accggcttcg cgtgcccggc ttttgctgtc
    24001 agaacgggaa gaccctcaca ttttcgccct catgaatggt ctgacccggc tccagtcgca
    24061 acagcgtgtc tgcctggctg agtccgaaaa gcgcgtggct catctgcacg ccctgcggga
    24121 taaagcgatc gtggcggagt tgaccgcgca gatagtgggg gcggtcgctg tcattgctca
    24181 aattccgtcc gcacaccgcc gggagtgatt ccggttcagt gtctgttcta ccaagcagcc
    24241 tgagcaaagc gggcctgacg aatagctgga aggtgacaaa gccggacaca gggtttcccg
    24301 gcaggccgaa aatgtggaca gcacggccct gccaggtggt ttgggcaaac aggaagggct
    24361 tgccgggttt cacctttacc cgccagattt ccggctcgat gccaagggcg cccagggcgg
    24421 gcttgacctg gtcgcgagcg cccacggaga ctccgccgct gactaggatc atgtcatgct
    24481 cacgaatgag tcttttgagt ttctccagtg tcgcgttgaa atcgtcttcg cagtgcgcgg
    24541 ctgtcgcgcc cctcaccccc gcctgagcca gcatggcccg cagcatgacg ccattgctgt
    24601 tgtaaatctg gcccggcagc agcgcggctc ccggcgggcg cagttcatcg ccagtggtca
    24661 gcagggcggc tttcggaggg gcatgcacgg gcaccgtggc gtgtccttgc gaggccagca
    24721 accccagtct gcctggagtg agggcatcgc ctgccttcag catgatctgc cccgcgcaga
    24781 ggtccgcgcc ggcgcggcgc acgttttcac cgggtgcaac cggttcgagg cagcggatgg
    24841 cgtcctccaa gacctgcaca tcctcctgca tgatcaccgc gtcagctcct gcgggaatgg
    24901 gcgcgcccgt gaagacacgg acagcttcgc ccggtccgca gatcaaggat tggtccagcc
    24961 ccgcgggctg gacacctttg agatttaaaa tcctttcacc cgtgctctcg gcagcccgca
    25021 gcgcgtagcc atccatcatg gaattgtcaa aacctggcag tggaaagtcc gcaagcatcg
    25081 tccgcgcgca cgagagtccg agtgcgtccg gcagcggcac ctcggcgact ggcaagggcg
    25141 tcacggcgtc gagtatgcgt cgcagggctt cggcttcgct gatcatggtt cggaggacgg
    25201 caaaggtgac gccgaagtct cgaatccctc aggcaggcct ggcaaggagg gcaggcttaa
    25261 ggtggagggt tgttgctggc cgggcggcat ggctggttga atcagtggag tcgcgggagc
    25321 cggggtgagt tttcctgcgg ccccggctct ctcgtgaatc accaggtgag tcacctccgg
    25381 ccggccttcc ctgatgtccg cggtgatata ccagcctttg cgctccgggg tgagcaccag
    25441 gcgcgatctc tctcccagag cggaaaccgc caccagttcc gtgccgggcg agaacgccgg
    25501 catttgctcg cagcggatca gcacatactc ctgctccggg ttcaccattt cgacgatgcc
    25561 gaccagaacg gtcgaatcgt catccttgtt ctccttgggc ggtttgtttt tcaatgccga
    25621 gcagcccgcc agcaggatgc atgcaagggc atgaactgga atagggggga gtgctggcat
    25681 gagctgagtc taaagccgtc gcatcggttt tggcgagaca ttttgcgtcc cgtcccctca
    25741 ctgggtgatc gcaaagacat gcagcgcggt gccgttggga tgcagttcca cataattctc
    25801 cgcgccctgc caatcatagg cttgatcatg catgagatca tgcgcgcggt agggtctggc
    25861 ggggtcgagg ccgagcgcgg cgaggttgag acgcgcctgt cccgccaccg gctggtggcc
    25921 gtccaggctg atcacgcaaa ggatgcggtc gctgaaatcc ggcgcgcatt tgctgaagca
    25981 cagcatggca ttgtggtcgg cgtcgtggaa gaccagattg tcatagagct gcagggcgcg
    26041 gtgttcctgg cggatacggt taagcctgcc gatgaagcct ttgatgttgc cgggcgcgtt
    26101 gtagtcgcgc tggcggagct ggaatttctc cgagtccaga tattcctccc tgcctggcag
    26161 cggctcgttt tcacagagtt cgtagcccgc gtacatgccc cacgaggatg aaagcagggc
    26221 ggccagcgcg gcgcgcatcc tgaacatgct ggccggcgcg ttttgcagat ggaagggatt
    26281 gatgtcaggc gtgttcggcc agaaattgcc gcggtaatac cagcgcatct cgccttgagt
    26341 cagctccctg gcgtattcgg cgagggcggc cttgtcctcg cgccaggtga aataggtgta
    26401 gctctggctg aagccgattt ttcccaggac ctgcatcatc ttcggcttgg tgaaagcctc
    26461 ggccagaaag atgacctccg ggtctttgcg atgcacggtt ttgatcaact cctcccagaa
    26521 agacacgggt ttggtgtgcg ggttgtccac gcggaagatt ttcacgccct tgtccaccca
    26581 gaagagcacc acgtcaatga gctcgcgcca caggttcttc cagtccgcgc agtgaaagtt
    26641 cagcgggtaa atgtcctggt attttttggg cggattctcc gcgtagcgga tactgccgtc
    26701 cggccgctga taaaaccact ccgggtgctc cctcacataa ggatgatccg gggagcagtt
    26761 gatggcgaag tcgagcgcga tttccaggcc gcgttttttg gcctcttcca gcagccagac
    26821 gaaatcgtcc agggtgccca gttttggctc gatctcgcga tgcccgcccg cgggtccgcc
    26881 gatggcccac gggctgccca catcgtcggc atgggcggtg agcgtgttgt ttttgccctt
    26941 tctggcagtg atgccgatgg gatggatggg agggaaataa atcacatcga agcccatcgt
    27001 ctttgcgtca tcaaggcgcg gcaggcagtc gcggaaggtg ctgtgccggt cgtagcggcc
    27061 ctcggcgccc cgcgggaaaa actcatacca tgccgagaac cgcgcccgct cgcgttcgac
    27121 aatgacgccg agaggctccg aggtcgtgga cagggagcgg tcggcatagg cgtccatgag
    27181 cacctgcagg tcgtttgatt gcagcatgtc cagaagctgc ggggctggca gtttttccag
    27241 aagatcggcg atgtcctcaa gctgtctgcc ggcgtcgcca gcgcccgccg ccc
//
LOCUS       DAOMYJ010000047        26864 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Prosthecobacter sp. isolate AMR_MDS_5629
            NODE_654_length_26864_cov_19.460219, whole genome shotgun sequence.
ACCESSION   DAOMYJ010000047 DAOMYJ010000000
VERSION     DAOMYJ010000047.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867850
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prosthecobacter sp. (wastewater metagenome)
  ORGANISM  Prosthecobacter sp.
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Verrucomicrobiaceae; Prosthecobacter.
REFERENCE   1  (bases 1 to 26864)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 26864)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.30
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:34:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,742
            CDSs (total)                      :: 4,684
            Genes (coding)                    :: 4,676
            CDSs (with protein)               :: 4,676
            Genes (RNA)                       :: 58
            tRNAs                             :: 55
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26864
                     /organism="Prosthecobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_654_length_26864_cov_19.460219"
                     /isolate="AMR_MDS_5629"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1965333"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2021
                     /locus_tag="PLP58_07075"
     CDS             <1..2021
                     /locus_tag="PLP58_07075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ08313.1"
                     /translation="CKAFLEVQRYPQAKVAVRRDGSSQPIFEREYPNRYYTTAALDES
                     ESIIVIIQIKVDADHPLEECVENKIAVEVIWLATGSTLFHEEDSYNCKEDLKAIEQGG
                     CAVSIDSLVQWIQIWVFSYSQFSDRLGVRKILISPNHEGSRWFHDFRHIEPCHLNSSI
                     FKLSGDGRHLLVATPYSAEFWEVRDGVPHAQLADIKFCDFGLIRRDDLLKGFVLESGF
                     EFEVSDVCGYAVLDTLRRFDFVRRGLLAVVAPGSFDNVSRQQKMYQDTLSALPAHCDS
                     EFIQNGVALFDAAMESPVADMRDLLNRRWAWTSSFPAKLGIKAWHVQTHDQYWARPVY
                     LVQSRVADEFIFLRDGAFPEFTCEPLRFAQHIVSNEVRQENYISLGPVSNNGHLTMRS
                     LTDRSSERLSRVELHQLDLNLQSETPICWLDVDCSVYEYLNGARLVGATDLAIFARTG
                     SNEITIVRTDDANHPKRIFLDASIAGIYPVLDSKGRSCKCLVSTDKPFQLVSAMEDGS
                     ISILRAAKIASSNFRVKAVSTNGLTLVIEKTNGEIVVASKLAVEWRETRIWNPFEHFM
                     SWVEFRQRESQRICAAFTTPHCWTLSHCGRLLACVCDMWLLVLDVQERRCLLKWKLPG
                     QGVKSMSFDLSGRWLALTHSGNVWEEFEVMWNPVLTDDSATQTPERSR"
     gene            complement(1997..2320)
                     /locus_tag="PLP58_07080"
     CDS             complement(1997..2320)
                     /locus_tag="PLP58_07080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ08314.1"
                     /translation="MMRCASPKAKIDGLLHNVVEDTSVTFEELEAEGFDAEILAALRL
                     LTHASEVSYEDYIQQVKTHPIATEAKLADLEDNMDIRRLETVDEKAITRFRKYLAAYR
                     DLSGV"
     gene            complement(2777..4621)
                     /gene="typA"
                     /locus_tag="PLP58_07085"
     CDS             complement(2777..4621)
                     /gene="typA"
                     /locus_tag="PLP58_07085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009959171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HRJ08315.1"
                     /translation="MTHPSKIRNIAIIAHVDHGKTTLVDSLLRASGNFRENQAIAERA
                     MDSMDLEREKGITIKAKNTSVHWNDHIINIVDTPGHADFGGEVERVMKMVDGVMLVVD
                     AYEGPQAQTRFVLKKALQQGLKPIVLINKMDRPHIEPHAVHDKVLELFLELEANDDQF
                     NAPFVYGSARAGWVTDKAGGEPHEMTYLLEQIVKHIPAPAADPNGSFEMLVSNIDWDN
                     FVGRVAIGKVTRGSVKMGDRVFLLGKTPVEKPKPVKVTKVFQYTTLTTSDSVEGGAGD
                     IVGISGFEDVDIGQTVAGSPDAEALPFVAIDPPTIVMQFAVNDGPLAGREGEHVTSRK
                     IRDRLDREQKMNVTISVEDTDTAGIFNVSARGAMQIAILVEQMRREGFEVLLSRPMVI
                     TKKINDTLCEPYETLYVDVPDDYLGGVMKSISERKGKIEDMSAHNGRTSLQACIPTRG
                     LIGFEFELMNLTSGHGIHSHLFKEYAPHAGHMQTRGSGTLVSSEGGEATAYALDTIQD
                     RGKLFVTAGDQVYEGMIVGENPRTDDLPVNPTRSKQLTNFRAAGSDKGIQLTPPIIFS
                     LERAIEYIAPDELVEATPKSIRLRKRILDSNVRLRERKRIEAELEAAA"
     gene            complement(4731..6350)
                     /locus_tag="PLP58_07090"
     CDS             complement(4731..6350)
                     /locus_tag="PLP58_07090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="IPT/TIG domain-containing protein"
                     /protein_id="HRJ08316.1"
                     /translation="MAAAAVLSCLAAALRAESPKLEALFPAGGRAGSSFVLAASGRVE
                     EGARLWTEAPGIHFVPTGKKREWQATVEAGVPAGLYLVHAANADGASAPRWFSVGTLP
                     EVAEVEPNNEAGQGMPLEKLPVCVNARLEQSGDVDGFKVKLAAGQAMAGLVEAYSLGS
                     PVDVMLHILDPGGIRVHTASDGRSLDPEFVFKAEKSGEHTVQIAGFAHPPQANVAFTA
                     GATVVYRLHLSTGPVVTRIHPPAIAAAGKTTVTLSGIGLDPRKLAHSIEAPAGARPGE
                     VIQVVVPGALQPQQVLVSESAPQQEKEPNNERDKATPVKAGVVAGKITGKEDSDRFSI
                     EMKKGEKLQASVWAKRLGLPLDAALTIEDPQGKVLSSIADAGDEADPAASWTAAVDGP
                     HQIVVRDQFNLGEEDGHYALEIGPARPGFTATLADAKALVIERGKSASLKVTVKLLNG
                     FKDPLTARVSGLPAGMHAPEVAVPEKGGEVEIKLHAAANAPAGGALTRVELWGKSEPA
                     RVTPASAAIRGEDKRGTTLRDDTNAVWAQVR"
     gene            complement(6374..7714)
                     /locus_tag="PLP58_07095"
     CDS             complement(6374..7714)
                     /locus_tag="PLP58_07095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009966004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1501 domain-containing protein"
                     /protein_id="HRJ08317.1"
                     /translation="MAIHPINCNGLGRRDFLQTGFGALGGLAMTDLLALRARAAQARG
                     KASPDQVNCILVWLDGGPSHYETFDPKPDAPQDIRGEFKSIPTCVPGVRFSEVVPKLA
                     QSFDKFTVVRSICHKDPNHGGGNHYLMTGAPTPVPVGCGAFVSFHPSFGSMVSHERGV
                     RGGLPAYMSLPSVSRSGGPNFLGAQHAPFVIGGDPNSKSFRVRDVAIPQDISEGRALS
                     RNELRASLDRMRRITDTAADDPTTGFDQFYRQGMELVTSPTAQAAFDLSREPEHVRKM
                     YGENDFGQRLLLARRLAEVGVSFTVAYWGGWDDHRGIFKAFKHEGKAAKLDQGLSGLI
                     TDLDQRGMLESTLVVCLGEFGRTPKVNKDAGRDHWPGAMSVLMAGAGIPGGQIVGATD
                     PKGYYAAENIYSPEDFAVSLYTKLGIDPHQVLHTSTGRPVQLVNGGKPIKELFA"
     gene            7814..8257
                     /locus_tag="PLP58_07100"
     CDS             7814..8257
                     /locus_tag="PLP58_07100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HRJ08318.1"
                     /translation="MIHQRLRRLLLPMTAMVFACLIPSCGSRPGAAIADPAFAKVAGV
                     LEANCVHCHGDNRLSHMPPINDSAELARLVGSNAWIVPGKPECSRFFQVVTFGDAIPG
                     AMPPTGHAIARQDVAILRHWITEGARIPEGRIISFHPRGKRPRSE"
     gene            8317..11415
                     /locus_tag="PLP58_07105"
     CDS             8317..11415
                     /locus_tag="PLP58_07105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009963454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HRJ08319.1"
                     /translation="MMPAKLISALAILAAPVLAAPQWIWLSNKAADGDQVTFQHHFDV
                     KLTPKSATLTATCDNGAEIYLNGKLLAVNPDWQMPVRADAIKHLVIGGKNHLVVKARN
                     KGGAAAFILKLELERQNGTVDVIETSAKWDATPTGKEDWRPAVVIAEYGAGPWGKALE
                     KGPGSAPKAAVTEVVAADQITAAPGFKVELLYNVPKEEQGSWVALTVDGKGRLIACDQ
                     YGALYRMNLPAIGITETLKPEKLPVEMGKAHGLLHAFGSLYVMVNENGKDNGLYRLRD
                     TTGDDQYDQIEKLVTMKGGGEHGLHSMVVSPDGKRIYFNGGNHTELPDELHASRPAKL
                     WAEDHILPRMWDANGHARGRMAPGGFICSMDPNGKNVELFCYGFRNEFDIAFDLGGEL
                     FTYDADMEWDIGSPWYRPTRVNHCVSGADYGWRSGSGKWPDYYPDSLPTTLDIGPGSP
                     TGVVSGQGAKFPAKYQRAVFIADWTYGTMWAIHLQPKGGSYLAEKEEFLFGKPLPLTD
                     VVIHPQDGAMYFAVGGRRTQSGVYRVTYAGEESTAPVKALPLDEEQRMRHTLERLHVD
                     GAAEPQKILDTAWPYLSHSDRHVRYAARVAIERLPRDLWQQRALGETHPQAIIECIIA
                     LARVHGGASEQLASKPEPGASSPPIAYVSADKAELQIEMLRTLGRLGRSQLSRGQTLA
                     ALRAIQLVFTRLGKPGPEVCDAVCEKLEPLYPSEDAMVNRELCQLLIALDSTAVPSKT
                     LALMATAADDFQEVASDAVLSRNDGYANAARAAAGSRPNAQQIAYIFALRNATSGWTP
                     ELRRTYFSWFPRARTWKGGNSFKGFIENTRKEALANFVPEDERAELDALSSRMESVQI
                     ANYVAPKGPGRAWSLDEATALATTGLKGRDFANGEAMYRATMCATCHRFNGDGGSIGP
                     DLTGSGNRYTLRDLMENIIDPSKVISDQYDSHEIVKKDGSVIIGRIVVEENEKVFIMT
                     NPFAPNDHISVDEKDILRKGTRPVSMMPPGLINALNPDELLDLTAYLLSGGNPQDKMF
                     QK"
     gene            11526..12239
                     /locus_tag="PLP58_07110"
     CDS             11526..12239
                     /locus_tag="PLP58_07110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ08320.1"
                     /translation="MKHPLLLLTALVFTSAMAADGPGELMFSDDFERGESQETKDEPG
                     NGWGTNSKSRAKGHKQVDLKDGVMRIFIHAEADHAVSVTHPAEFTDGAVSLRFMLEDE
                     RDSLGLNFADLEFKEVHAGHLFVAKVSPKSVQLQDLKTGNMRLDIREARQAKQKLSAE
                     QEAAVKGKDKIFPHAVETGKWHELVVTISGDTLSASIDGKPAGTFSSPGIAHPTKRTL
                     RLAVPRNAVVDDVKIWRKK"
     gene            complement(12246..13304)
                     /locus_tag="PLP58_07115"
     CDS             complement(12246..13304)
                     /locus_tag="PLP58_07115"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF021778.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052689 - carboxylic ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lactonase family protein"
                     /protein_id="HRJ08321.1"
                     /translation="MKAILTFLCLVTSALAGPTIVYVSESGEKRIAVWSLDESSGELT
                     RLSDATLPGAPGSLAISPDRQHLYASVRSAKQFATLDVDVKTGALSNASFADAGFNAA
                     YVFADKTGRWLLAASYSEGVVGLSATQNGRVSGLPVVTLETGKKAHCIQVDPANRFAF
                     VPHVGELNKVEQLRFDAQAGTLTRNRPPHLPGGAGEGPRHMQFHPNGKWAYFVNEQGK
                     SVTLCDYDAANGRLKARQSVPTVPEDWRDKGSCADIHVSADGRFVYASNRGHDSLAVF
                     SVNAQTGELTAHGQTPTEKTPRSFCLTPGDRFVVSAGEGSHKLIVFRRNAETGTLITL
                     KTYDCGKGPAWVTSVKFD"
     gene            13613..17095
                     /locus_tag="PLP58_07120"
     CDS             13613..17095
                     /locus_tag="PLP58_07120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ08322.1"
                     /translation="MNTPALLSALAKHGLGWPRYFAKAAVYLTGFIGLFASAEVISSG
                     SQVARALAGQAEKGLRVTATDDGEARLHCAFQKIAAEATPAGLWLTSLSGAVPDRFQI
                     RADRAGRGGEFHELEEKGGVIVTDQKAVWARTGLIEEYSVSVDGVRQDFLVTERPAGG
                     GALQVELAALGCRAGGGGDGALLTLAGSGRQIAYHRLRVTDAAGRLLRARMTATAPDR
                     LVITVEDAEAVYPVRIDPTFADADWNSLGGLPGTNFVVYGAAVDSAGRLFLGGGFSVA
                     GTTQAQNIVMWDGSAWSALGEGLNGTVYSVAVDSNDHVYATGTFSHTGDGALVLNGVG
                     KWNGTAWEALGTGLGGTFPHGRALAVDDSDDVYVGGLFTTAGGVTSPDIAKWDGSGWS
                     ALGNGSNGTINAVAVDGADVYVGGTFSAMGVVSANKVARWDGSAWSALGSGLGTVNQV
                     VNSLAVSSGGTVYAAGSFTSSGGLPVNGIAEWSGAVWNEVGGGLDGPGTTCAFDTAGN
                     LYVGGAFETAGAVSARNIAKWNLLTTTWTALDTGTDNSVQVIVSDASGGVFAGGSLTT
                     AGSTGARSIAHWDGAAWSALHSGINDGVLAFAADSAGRVYAGGDFVRAGTTLASHVAM
                     WDGTAWTALGTGVNGKVLALAVDASDDLYVAGEFTLAGGASANRVAKWDGSAWSALAD
                     GLDDRVSALALDGGGNLYAGGSFLNSGAAPMARVAMWNGTGWSALGTGAASGSVNALL
                     WSSSDSTLYAGGGFTIMGGVTVNRAARWNGTAWSALGQGFNGTVNTLALSPGGVLHAG
                     GTFTQSGTQTRNRVARWTGTSWAALGSGNGGTIHTMTFDISGTLYVGGSFSSAGGGAA
                     NNIAHWNGVSWFAIGSGTNLPVHALLAHPAGRLYAGGYFSLAGDKVSGYAAWLDIAAP
                     EIAVFNGASTAPADERNDGSGFWDFGTQARDSVSAPQTFTIKNVGSATLSGLAVVLDE
                     DWDDDHFSITQPALTSLLPGESTTFTLAFAPLALGDLFGVVQILNNDENESPFLISAS
                     GSGVWTIQQWRQFYFGTSDNSGNAANSFDPNFDGVPNVVAYALGLHPLENSAHLLPVA
                     DPGDLEAGWLSLFVDEGPGVVGVTVGAETSETLEVEDWTPATEYGDGPYHYLSEQIGL
                     RDRLFIRLTIDVME"
     gene            complement(17163..18581)
                     /locus_tag="PLP58_07125"
     CDS             complement(17163..18581)
                     /locus_tag="PLP58_07125"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase"
                     /protein_id="HRJ08323.1"
                     /translation="MKRLLILLSSFAIHLSSLSAKPNVLFIISDDLTFTALGCYGNKV
                     CQTPNIDRLAARGMRFTRAHCQGTYCGPSRASFMSGYYPHATGVQGYISPRAAIGERA
                     TWAQHFKNAGYYSARVSKIFHMGVPGGIEKGGDEADDPASWTERFNSQGPEWQAPGIG
                     ETLEKNPDGKKPVMGGNTFVIVAADGDDLVHSDGKTAAKACELIAQHAKEPFFLGVGF
                     VRPHVPFVAPKKYFDMYPWEQMMLPEKVPGDWDDIPKPGINYKTSVNMQMDVTRQKKA
                     LAGYYAAVSFMDAQVGKVLDALEKSGQADNTIIIFTSDHGYHLGEHDFWAKVSLHDES
                     ARAPLIISVPGKKPGVSDSLVELLDLYPTTAKLCGLEVPARLQGKDISPILDDPQARV
                     HDTVFSVAGSSKGLMLRDERWVFIQYTEDASKGIELYDMHNDPRQLTNLASSPDHVSR
                     IEEWKAKVAAKLAAVRANDLGQ"
     gene            complement(18625..19635)
                     /locus_tag="PLP58_07130"
     CDS             complement(18625..19635)
                     /locus_tag="PLP58_07130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ08324.1"
                     /translation="MNFTLLRIDAKSEVQTLNWLGDCLIDLASGGQLYDLDGTITPNQ
                     LFWGGPAFDMCVTSPSGRYAVLCQRLGTKGLVIDLAGELPKLLREINRSYYCSQAFEY
                     PVSFVGNQKTEWLVHCPEDYNHLEIEEVETGASVLDSVKSKLRKPADFFHSRLEASPN
                     GQCLISAGWVWHPFDTIAMFNLEGVCSNPGALDSISFPGLADWGEVNSAAFVDDETVL
                     VSENSSRVTYGEGDKTVARIRAISLTKGETVHEFEIELAAGTMMALGTDHVVSFYQHP
                     KVYHLPTGRLIASWPEIRSGQDSSSIIHHLEKAPAIARDRQNQRFAVANGKEIVVVQA
                     FP"
     gene            complement(19651..21612)
                     /locus_tag="PLP58_07135"
     CDS             complement(19651..21612)
                     /locus_tag="PLP58_07135"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HRJ08325.1"
                     /translation="MKTLAFASSFLIASLRSPLWGSKRFALLAVSLCSALVSSLPAKP
                     NVIYLMADELGYYEPAFMGGKNIQTPNLDRMAAEGMRFNNLLAGSSVCAPTRCCFLTG
                     KHSGHTSVRSNGGGTPLRADEATIASILKPLGYATGGFGKWGNGGRDSTGVPEKHGFD
                     TFLGYYDQVHAHSYYPPYLIQNSEEVPLEGNQGATGKTYSHYVIHDAAMKFIRDHAKQ
                     PFFAYLPYTPPHGNFQIPDTDPAWALYQDKPWPEDARRYAAMVTMIDRQVGEIIALLQ
                     ELGIEERTLVMFSGDNGANDYFKSAEHPRGVHSGNKDPQSGVEYRGTKGTLYEGGLRI
                     PFIARWPGKIAPGAVSDHLGYFPDILPTVAAVTGATAPADIDGLSLLPTLLGKNAEQK
                     QHEYLYWEISGWTAIRQGKWRAVRKKPTAPWELYDLATDPAESKDIAATKPDVLAKLT
                     ALAEKAHEPAREGTFSRTDRHERDRRAKFGKQDDLNYNPTPGGGKKKSAKSTAMDPVK
                     GMLSSKEWKVVHVSSENTGNSKFAANAIDGDPDTLWHSRFSKPAAKPPHELVIDLGAP
                     HQIRGFVYLGRQDAGWNGAAKDVEFAISDSPGAFGSAAVKTSLQKIKTPQTIACTPAT
                     GRYVLLRILSEHREINELASAAEIGVLGE"
     gene            complement(21609..21905)
                     /locus_tag="PLP58_07140"
     CDS             complement(21609..21905)
                     /locus_tag="PLP58_07140"
                     /inference="COORDINATES: protein motif:HMM:NF019491.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dabb family protein"
                     /protein_id="HRJ08326.1"
                     /translation="MIRHTVTFRLKHAPGSGAERDFLRAACALAGIEGVGNFECLRQT
                     SPKNLFTFGLSMEFADESAYAFYNNHPDHTAFVQNRWIPEVEDFLELDYMPHTP"
     gene            complement(21939..23165)
                     /locus_tag="PLP58_07145"
     CDS             complement(21939..23165)
                     /locus_tag="PLP58_07145"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialidase family protein"
                     /protein_id="HRJ08327.1"
                     /translation="MRLIFLPLLLASSVFSQTKLQKADVTLAPPKNLKTTLAPGYTIP
                     VVDISGETNRQVIVDREKGQYLGHPTTVLLEDNQTMLAVYPKGHGRGAIVYKRSNDAG
                     LTWSDRLATPKNWETSLEVPTLHRVVDAAGKKRLIMFSGLYPIRMAVSEDDGANWSEL
                     KAIGDFGGVVTMSTVLALKTPGHYLAFFHDDGRFIRGGEVEKYRVKSPHAPDYSSSTA
                     KPWRFWVYTTLSKDGGLTWSVPAPIAMLPDASLCEPGALRSPDGKQIAVLLRENSRKY
                     NSFVIFSDDEGLTWSHPKELPAALTGDRHVAKYAPDGRLFISFRDTTHVSPTKGDWVG
                     WVGKYEDIVNGAEGQYRVRIMDNKKGQDTTYPGVELLPDGTFVTTTYGHWTEGEEAYV
                     VSVRFKLGELDARLPR"
     gene            complement(23252..24196)
                     /locus_tag="PLP58_07150"
     CDS             complement(23252..24196)
                     /locus_tag="PLP58_07150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009959512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HRJ08328.1"
                     /translation="MASLLKTLGLAADPTRARLLLLLRQEELSVAELQEILALPQSNI
                     SAQLSRLKAAGLVSDRRSGKNRLYSLAPPPAREAPAREHFLALLEAAGASMPEVKKDA
                     TALKVSLKKRARAAQAYFDALAGKFGRHYIPGRSWKALGETLLKLMPPLVIADLGAGE
                     GTLSQLLAQRARRVIAVDSSEKMVAYGSALAREHGFANLEYRLGDLDDPPIDPASVDL
                     VILSQALHHAANPQKALRAAHRLLKPGGRIAVLDLLKHAFEKARELYADVWLGFSEAE
                     LLGMLEAAGFASVETGIVHKESRSPGFQTVLAIAEKKA"
     gene            24342..25049
                     /locus_tag="PLP58_07155"
     CDS             24342..25049
                     /locus_tag="PLP58_07155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ08329.1"
                     /translation="MVKRALIACVLALGAPARPAAAQEARAPEPQTLELMRLAFQKRL
                     MEAALNPLKQQLTSLAELEKQRAAAGDYAAAIEARDQRKRLTTELERLDKELLLAQTR
                     EQSLKARLLPERVSLDLAHAELDGGVRMDGGALTGWSRPGAAAKWKLPDLPPGGYEVV
                     VRYRCGPLEGGSLQVAEARFTLDADVVTTLRGPQEKNLGTLKVTDGSGPLVISARSVV
                     KDNLMHLLAVHLTPAGR"
     gene            25054..26514
                     /locus_tag="PLP58_07160"
     CDS             25054..26514
                     /locus_tag="PLP58_07160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ08330.1"
                     /translation="MKITAPIFLAILSCSSAQTTDPAPLASARAAFLRDVLASSKTLS
                     GQYESALARAEAELAAAAEYEEALLVRRRRDELKALYPVTGAEIAASLAIPLPARQAR
                     LSGSVEARGEELTQWRTNGSSAEWTGVKVSPGRYYLELEACLIETAPPPDGMLPGLFR
                     PQEHVTFEFHELSLLPGAAENRRSFEIAPARADGAFTSLQAGPVTFTRSPVSLRLTAA
                     AGYPARHIRLRALRLIPAAQDAPTIVSPDSSTPTLDDLRKSLSETLAAVQKPIVDGYL
                     QNLRDLAAGTPALKDAADAEARRIIRMLQDNGRSTQRLLGAATPLSAFDDLDGAVFVP
                     DPANEGDRFFVEHDGSRLRVQLLWVECAPVDAARDADRAGRIREHFRIEDTDSPARLA
                     REFTAGYLEGKPLRLLVRPGKNKDGSISALVFLPEVGLYQNILVDQGLAAVLAPREKR
                     GVSETGLHDSLLARERAAQRRKPAPGAWALSEGARR"
     gene            26535..>26864
                     /locus_tag="PLP58_07165"
     CDS             26535..>26864
                     /locus_tag="PLP58_07165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ08331.1"
                     /translation="MRRLFSATLALLIAFEAGAQTPATHSTPAPSSGEDTRLRQMEAI
                     YVQQLRSLHVPLLGKYVTELQRLAAQAADPAPYQREITRIQDIISSGGVVDLADAARM
                     LKAPAEPP"
BASE COUNT         4763 a         8325 c         8654 g         5122 t
ORIGIN      
        1 cgtgcaaagc attcttggag gtgcaaagat atcctcaagc caaagtggcc gtccgccgcg
       61 atggcagctc ccaaccaatc tttgagcgtg agtatcccaa ccgatactat accacagctg
      121 cattagacga atcagaatca attatcgtta taatacaaat caaggtggat gcagatcatc
      181 cacttgaaga gtgcgtagaa aataagatag cggtagaagt catttggctc gccaccggca
      241 gcactttgtt tcatgaagaa gacagctaca attgcaaaga agacttgaag gctattgagc
      301 agggaggctg cgccgtttcc attgattcgc tggttcagtg gatacaaatt tgggtattta
      361 gttatagcca attttcagat cggctagggg taagaaaaat attaatatcg cccaatcatg
      421 aaggatctcg ttggtttcat gattttcggc atattgaacc ttgccatctc aactcgtcga
      481 ttttcaagct gtctggtgat ggacgtcatc tcctcgtcgc aacaccttat tccgcagagt
      541 tttgggaggt tcgggatggt gttccccatg cgcagctcgc agatataaag ttttgtgact
      601 tcggcctgat tcggcgtgac gatttgctca aaggttttgt gcttgaatca gggtttgagt
      661 tcgaagttag tgacgtttgt ggatacgccg ttctagacac cctacggcgc tttgactttg
      721 tgaggagggg gctactagcc gtggttgctc cgggaagctt tgataatgtc tcaagacagc
      781 aaaaaatgta ccaagataca ctgtctgcac tcccggctca ctgcgactct gagttcattc
      841 aaaatggagt ggcactattc gatgcggcaa tggaatctcc ggtggccgat atgcgcgatc
      901 ttttgaatcg ccgatgggct tggacgagca gtttcccagc aaagcttgga ataaaagcgt
      961 ggcacgttca aactcacgat cagtactggg cccgcccggt ttatttggtc cagagccggg
     1021 tagcagacga gttcatcttt ctgagggacg gggcgttccc tgaatttaca tgtgagccgc
     1081 ttcgttttgc tcagcatatt gtcagtaatg aagttcgaca ggagaactat atctcacttg
     1141 ggccagtgtc caataacggg catctcacca tgcgttcatt gacagatcgt agtagcgaaa
     1201 gactttcgcg tgtcgaactc catcaactgg accttaatct acaatccgaa acacctattt
     1261 gttggctcga cgttgattgc agcgtgtatg agtacttgaa cggtgctcgg ttagtgggtg
     1321 ccacggatct cgcaatcttc gcaagaacgg gctcaaatga aattacaatc gtcaggaccg
     1381 acgacgcaaa ccacccaaaa cgaatctttt tagatgcgtc tattgccggc atctatcctg
     1441 ttctcgatag taaggggcgc agttgcaaat gccttgtttc gactgataag ccttttcaac
     1501 tggtcagcgc gatggaagat ggcagcatct ctattctgag ggcggcgaaa attgccagtt
     1561 caaattttag ggtgaaggcg gtttcaacta atggtttaac cttggttatt gaaaagacta
     1621 atggagaaat tgtagtcgca tccaaattgg ccgttgagtg gcgtgaaacg cgcatctgga
     1681 atccttttga gcacttcatg tcctgggtgg agttccgaca gcgcgagtcg cagcgcatct
     1741 gtgccgcctt taccacaccg cattgctgga ctttaagtca ctgcggccgt ttactggcgt
     1801 gtgtttgtga tatgtggttg ttggtgcttg atgtgcagga gcgccgttgt cttttgaaat
     1861 ggaaactgcc tggtcagggg gtgaaaagca tgagcttcga cttatccggc agatggcttg
     1921 ctctcaccca ctcgggaaat gtttgggagg aattcgaagt gatgtggaac ccggttctca
     1981 cagatgattc cgcgactcaa acgccagaaa gatcacggta ggctgctaaa tactttcgaa
     2041 atcttgtaat ggccttttcg tcgaccgtct ccaaccgacg tatgtccatg ttgtcctcca
     2101 gatcggcgag cttcgcctcc gtggcgatgg ggtgggtctt cacttgctgg atgtagtctt
     2161 catatgacac ctcggaagcg tgtgtcagca gcctcagcgc agccaggatt tcggcatcga
     2221 agccctcggc ttccagttcc tcaaatgtca cggaggtgtc ctccaccacg ttatggagca
     2281 gtccgtcgat ctttgccttg ggtgacgcgc agcgcatcat cacccggatg ggatggaaga
     2341 tgtaagcggc accgcttttg tccttttgcc cagcatgagc ttggacggcg atttaagtgc
     2401 tcgttcgagt gtggccataa agtaaaaagt gcagatgttg agattattcc cccacggtgc
     2461 gggaaagtaa cctgttttgc aagaactgcc gccaaagtgg ttcgatgtga tccaagcaag
     2521 tgttgaggcg gtggtcgccg tcgagcaggt gcagctcgac gcgctggctt tcggcaaagc
     2581 ggacagagtg cgaccatgga atgatgtcat cacgccagcc gtggaccacg gcgacggggc
     2641 agggaagcgg cgggtattcc tgcaccgcat aacccggcct tgcgggagtt ttttcatctc
     2701 gcgcgttcgg cggccatcat gctcacgcct cacccggcca ggggtggatg tcatgctccg
     2761 catgaaaact ccgtcatcat gcagccgcct cgagttcggc ctcgatgcgc ttgcgttcgc
     2821 gcaagcggac attggagtcg aggattcgct tgcggagccg gatgctcttc ggcgtggctt
     2881 caaccaactc gtcaggggcg atatactcaa tggcacgctc caggctgaag atgatgggcg
     2941 gtgttagctg gatgcccttg tcgctgccgg ctgcccggaa gttggtgagt tgtttgctcc
     3001 gggtgggatt gaccggcaga tcgtcagtgc gcggattttc acccacgatc atcccctcat
     3061 agacctggtc gcccgcagtg acgaagagct tgccgcgatc ctggatggtg tcgagcgcat
     3121 aggcggtggc ctcgcccccc tcgctgctga ccagggtgcc ggagccgcgt gtctgcatgt
     3181 ggccggcgtg cggggcgtat tccttgaaaa ggtggctgtg gatgccgtgg ccgctggtga
     3241 ggttcatcaa ttcgaactca aaaccgatca gtccgcgcgt gggaatgcaa gcctgcaggc
     3301 tggtgcgccc attgtgagcg ctcatgtcct cgatctttcc cttgcgctcg gagatgcttt
     3361 tcatgactcc gccgaggtag tcgtcgggca cgtccacata cagggtctca tagggctcgc
     3421 agagcgtgtc gttgattttt ttggtgatga ccatcgggcg ggaaagcagc acctcgaagc
     3481 cctcgcggcg catctgctcg acgaggatgg cgatctgcat ggcgccgcgc gcgctgacat
     3541 tgaagatgcc cgctgtgtcg gtgtcctcca cgctgatggt gacgttcatc ttctgctccc
     3601 ggtcgagccg gtcgcggatt ttgcgcgagg tgacatgctc gccctcgcgc ccggccagcg
     3661 ggccgtcatt gaccgcgaat tgcatgacaa tggtaggagg gtcaatagcc acaaaaggca
     3721 gcgcctccgc gtcgggactg ccggccacag tctgcccgat gtccacatcc tcgaagccgc
     3781 tgatgcccac gatgtccccc gcgccgccct cgacgctgtc gctggtggtc agcgtggtgt
     3841 attggaaaac ttttgtaacc ttgaccggct tcggcttctc aaccggcgtc ttgccgagca
     3901 gaaagacgcg gtcgcccatc ttgacggagc cgcgcgtgac cttgccgatg gcgacacggc
     3961 ccacgaaatt gtcccagtca atgttcgaga cgagcatctc gaagctccca ttgggatcgg
     4021 cggcgggcgc ggggatgtgc ttgacgatct gctcgagcaa ataggtcatc tcatgcggct
     4081 cgccgcccgc cttgtccgtg acccagccgg cgcgcgcgct gccatagaca aagggcgcgt
     4141 tgaactggtc gtcgttcgcc tccagctcaa ggaaaagctc cagcaccttg tcatgaactg
     4201 cgtgcggctc gatgtgcggg cggtccattt tgttgatgag gacgatgggc ttgaggccct
     4261 gctggagggc cttcttcaag acgaagcgcg tctgcgcctg cggcccctcg taggcgtcca
     4321 cgacgagcat cacgccgtcc accatcttca tcacgcgctc cacctcgccg ccgaagtcgg
     4381 cgtggccggg tgtgtccacg atgttgatga tgtggtcgtt ccagtggacc gaggtgttct
     4441 tggccttgat ggtgatgccc ttctcgcgct ccaggtccat gctgtccatg gcgcgctcgg
     4501 cgatcgcctg gttctcgcgg aagttgccgc tggcgcgcag caggctgtcc accagcgtgg
     4561 tcttgccgtg gtcaacgtgg gcgatgatgg cgatgttgcg aatcttggag gggtgcgtca
     4621 ttgggggctt tttggggggg aagggggcgc ggagattagc cgctttcccc cctttggcaa
     4681 acgagggttt cggcactcca ccggctgttc atccgcggcg ctggaatgcc tcacctcacc
     4741 tgcgcccaga ccgcgttcgt gtcatcgcgc agcgtggtgc cgcgcttgtc ctccccgcgg
     4801 atcgcggcgc tggcgggcgt gacgcgggcg ggttcggatt tgccccacag ttccacccgc
     4861 gtcagcgcgc cgcccgcggg cgcgttggcg gcggcgtgca gtttgatctc cacctccccg
     4921 cccttctcag gcacggccac ctccggcgcg tgcatcccgg cgggcaggcc gctgacacgg
     4981 gcggtgagcg ggtctttgaa gccattgagc agcttcaccg tgactttgag gctggcgctt
     5041 ttcccgcgct caatgacgag cgccttggcg tccgcgagcg tggcggtgaa gccgggacgc
     5101 gcggggccga tctccagcgc gtagtgtccg tcttcctctc cgagattaaa ctggtcgcgc
     5161 accacgatct ggtgcggccc gtccacggcg gcggtccatg aagcggcggg gtccgcctcg
     5221 tcaccggcgt cggcgatgct ggagagcacc ttgccctgcg ggtcttcgat ggtcaaagcc
     5281 gcgtccagcg gcaggccgag ccgcttcgcc cacacggagg cctgcagttt ctcgcctttt
     5341 ttcatttcga tggaaaagcg gtcgctgtcc tccttgccgg tgatctttcc cgccacgacg
     5401 ccggctttga ccggcgtggc tttgtcacgc tcattgttcg gctctttctc ctgctgcggc
     5461 gcgctttccg acaccagcac ctgctgcggc tgcaaggcgc ccggcaccac cacctggatc
     5521 acttcccccg gccgcgctcc cgccggcgcc tcgatgctgt gcgccagctt cctcgggtcc
     5581 aggccgatgc cggaaagcgt gaccgtggtc ttgcctgcgg ccgcgatggc ggggggatgg
     5641 atgcgcgtga ccaccggccc tgtgctcaaa tgcagccggt aaaccaccgt ggcgccggca
     5701 gtgaaggcca cgttcgcctg cggcggatgc gcgaaccccg cgatctgcac cgtgtgctcc
     5761 ccggacttct ccgccttgaa gacaaactcc ggatcgagac tgcgcccgtc gctggcggta
     5821 tgcactcgga tgcccccagg atccaggatg tgcagcatca catccaccgg actgccgagg
     5881 ctgtaggctt ccaccagtcc ggccatagcc tgtccggcgg cgagtttgac tttgaagccg
     5941 tccacatccc cgctctgctc caggcgcgcg ttcacgcaga caggcagctt ctcaagcggc
     6001 atcccctgtc ccgcctcatt gttcggctcc acttccgcca cctccggcag cgtgccgaca
     6061 ctaaaccacc tcggcgcgga ggcgccgtcc gcgttggccg catgcaccag ataaagcccc
     6121 gccggcacac ctgcctccac cgtcgcctgc cactcgcgct ttttacctgt cggcacgaag
     6181 tgaatgcccg gcgcctcagt ccacagccgc gcgccctcct cgacacgccc gctggcggcc
     6241 agcacgaatg acgatcccgc ccgcccgcct gcggggaaga gcgcctccag cttcggcgac
     6301 tcggcgcgca gcgccgccgc caggcacgac agcactgcgg cggcggccat cacggctggg
     6361 atgcgcgcct gcctcacgca aagagttcct tgatcggctt gccgccgttg acgagttgca
     6421 caggccgccc ggtgctggtg tgcagcacct ggtgcgggtc aatgcccagc ttggtgtaga
     6481 gggacacggc gaagtcctcc ggcgaataaa tgttctccgc cgcgtagtag ccttttgggt
     6541 ccgtcgcccc cacgatctgc ccgccgggga tgcccgcgcc cgccatcagc acgctcatcg
     6601 cgcccggcca atggtcgcgc cccgcgtcct tgttcacctt cggcgtgcgg ccgaactcgc
     6661 ccaggcacac caccagcgtg ctctccagca tcccgcgctg gtccagatcg gtgatcagtc
     6721 cgctcagccc ctggtccagc ttggccgcct tgccctcgtg tttaaaggct ttgaaaatgc
     6781 cgcgatgatc gtcccacccg ccccagtagg ccaccgtgaa ggatacgccc acttccgcaa
     6841 ggcggcgcgc cagcagcagg cgctggccga agtcgttctc gccatacatc ttgcgcacat
     6901 gctccggctc cctgctcaga tcaaaggccg cctgcgccgt cggagaagtc accagctcca
     6961 tgccctgccg gtaaaactgg tcaaagcccg tcgtcgggtc atccgccgcc gtgtccgtga
     7021 tgcgccgcat ccggtccagg gaggctcgca actcgttgcg gctcagcgcg cgcccctcgc
     7081 tgatgtcctg cgggatggcc acgtcccgca cccggaatga tttgctgttc ggatcaccgc
     7141 cgatcacgaa cggcgcgtgc tgcgcgccca ggaagttcgg tccgccggag cgcgagacgc
     7201 tcggcaggct catgtacgcg ggcagaccgc cgcgcacacc gcgctcgtgc gagaccatcg
     7261 agccgaacga ggggtgaaaa ctcacaaacg ccccgcagcc caccggcacc ggagtcggcg
     7321 cgccggtcat caggtaatga ttgcccccgc cgtggttcgg gtccttgtgg cagatcgagc
     7381 gcaccacggt gaatttgtcg aagctttgcg ccagcttcgg caccacctcg gagaaacgca
     7441 cccccggcac gcatgtgggg atggatttga actcgccccg gatgtcctgc ggcgcgtccg
     7501 gcttcggatc aaacgtctca tagtgggagg ggccgccgtc cagccagaca aggatgcagt
     7561 tgacctggtc cgggctggcc ttgccgcgcg cttgcgccgc ccgcgcgcgc agcgccagca
     7621 gatcggtcat ggccaagccg cccagcgctc cgaagccggt ctggagaaaa tcgcgccggc
     7681 cgaggccgtt gcagttgatg gggtggattg ccattggggg agaagcgggg cagactacgc
     7741 ccgccgcggc cgcgttcttg cggccttgaa atcggcatgg cgcgccgccc cgccttctgt
     7801 tatgacaaaa gccatgatcc atcaaagact gcgccgactg ttgctcccga tgactgccat
     7861 ggttttcgca tgcctcattc ccagttgcgg ctcaaggccg ggagcggcaa tcgccgaccc
     7921 ggcttttgct aaagtcgcgg gcgtcctgga ggcgaactgc gttcactgcc acggcgacaa
     7981 ccgcctctcc cacatgccgc cgatcaacga cagtgcggag ctggccaggc tcgtcggttc
     8041 aaacgcttgg atcgtccctg gcaaaccgga gtgtagccgc ttcttccagg tcgtcacttt
     8101 tggcgacgct atccccggtg ccatgccgcc cacgggccat gcaatcgcgc ggcaggatgt
     8161 cgcgattctg cgtcactgga tcacggaagg agcacgcatc cccgaaggtc gtatcatttc
     8221 gttccatccg cgaggaaagc gtccgcgttc cgaataaagg catgaggcgc gtcgaggttc
     8281 agtttgaccc gccgccgcct cgcgctccat tcggccatga tgccagccaa actcatctcc
     8341 gccctcgcca tcctcgccgc tccggttctc gccgctccgc aatggatctg gctatccaac
     8401 aaagccgcag acggcgatca agtcactttt cagcaccact tcgacgttaa actcacaccg
     8461 aaatccgcca ctctcactgc cacctgtgac aacggagcgg aaatttacct caatggcaag
     8521 ctgctggccg tcaatcccga ctggcagatg ccggtgcggg cggatgcgat caagcacctg
     8581 gtcattgggg ggaaaaacca tctcgtggtc aaggcgcgca acaagggtgg cgcggcggca
     8641 ttcatcctga agctggagtt ggagaggcaa aacggcacgg tggatgtgat cgagacaagc
     8701 gccaagtggg atgccacgcc caccggcaaa gaagactggc ggcccgcagt cgtcatcgcc
     8761 gaatacgggg ccggaccgtg gggaaaggcg ctggagaaag gccccggcag cgcgcccaag
     8821 gccgcagtca ctgaggtggt ggcagccgac cagatcaccg ccgcgcccgg cttcaaggtg
     8881 gagctgctct acaatgtgcc gaaggaggag cagggctcct gggtggcgct caccgtggac
     8941 ggcaagggcc ggctcatcgc ctgcgaccag tatggcgcgc tctaccgcat gaacctgccc
     9001 gccatcggca taaccgagac tttgaagcct gagaagctgc cggtggaaat gggcaaggcg
     9061 cacgggctgc tgcacgcctt cggcagcctg tatgtgatgg tcaatgagaa cggcaaggac
     9121 aacggcctct accgcctgcg ggacacgacg ggcgacgacc agtacgacca gatcgagaag
     9181 ctggtgacca tgaagggcgg cggcgagcac gggctgcaca gcatggtcgt gagcccggac
     9241 ggcaaacgca tttatttcaa cggcggcaac cacacggagc tgcccgacga gctgcacgcc
     9301 agccgcccgg ccaaactctg ggccgaggac cacatcctgc cgcgcatgtg ggatgcgaac
     9361 ggccacgcgc gcgggcgcat ggcgcccgga ggcttcattt gcagcatgga ccccaacggc
     9421 aaaaacgtgg agcttttctg ctatggcttc cgcaatgagt tcgacatcgc ctttgatctg
     9481 ggcggcgagc ttttcactta tgacgcggac atggagtggg acatcggcag cccctggtac
     9541 cggcccacgc gcgtgaacca ctgcgtcagc ggcgcggact acggctggcg ctcgggcagc
     9601 ggcaagtggc cggattatta ccccgactcg ctgccgacga cgctggacat cggccccggc
     9661 agccccaccg gcgtggtcag cggccagggc gcaaagttcc ccgccaagta ccagcgcgca
     9721 gtcttcatcg ccgactggac ctacggcacc atgtgggcca tccacctcca gcccaagggc
     9781 ggcagctacc tggccgaaaa agaagagttc cttttcggca aaccactgcc cctcaccgat
     9841 gtcgtcatcc atccgcagga cggcgcgatg tacttcgccg tcggcggcag gcgcacgcag
     9901 tcgggagtct atcgcgtcac ctatgccggg gaggaatcca ccgcgcccgt caaagccctg
     9961 ccgctggatg aggagcagcg catgaggcac accctggagc ggctacatgt ggacggcgcc
    10021 gccgagccgc aaaagatcct cgacaccgcc tggccctacc tcagccacag cgaccgccat
    10081 gtgcgctacg cggcgcgggt ggccatcgag cgcctgccgc gggatctctg gcagcagcgc
    10141 gccctgggag agacgcaccc gcaggccatc atcgagtgca tcatcgccct ggcccgcgtc
    10201 catggcggcg cctcagagca gctcgccagc aagccggagc ccggcgcatc ttcaccaccc
    10261 atcgcctatg tgagcgcgga caaggccgag ctgcagattg aaatgctgcg caccctgggc
    10321 cggctcgggc gctcccagct cagccgcggc cagacgctcg ccgccctgcg cgccatccag
    10381 ctcgtcttca cccgcctggg caaacccggc cccgaagtct gcgatgccgt ctgcgaaaag
    10441 ctggagccgc tttacccctc cgaggacgcc atggtgaacc gggagctttg ccagcttctc
    10501 atcgcgctcg actccaccgc cgtgccctcc aaaacgctgg ccctcatggc caccgccgcc
    10561 gatgatttcc aggaagtggc cagcgatgcc gtgctcagcc gcaacgacgg ctacgccaac
    10621 gccgcccgcg ccgccgccgg cagccgtccg aacgcccagc agatcgccta catcttcgcg
    10681 ctgcgcaatg ccacgagcgg ctggacgccc gagctgcgcc gcacctactt ctcctggttc
    10741 ccccgcgccc gcacctggaa gggcgggaac agcttcaaag gcttcatcga aaacacccgc
    10801 aaggaagccc tcgccaactt cgtccccgag gacgagcgcg ccgaactcga cgctctcagc
    10861 agccgcatgg aaagcgtgca gatcgccaac tatgtcgcgc ccaagggacc gggccgcgcc
    10921 tggagcctgg acgaggccac cgcgctcgcc accaccggcc tcaaagggcg ggacttcgcc
    10981 aacggcgagg ccatgtaccg cgccaccatg tgcgccacct gccaccgctt caacggcgac
    11041 ggcggcagca tcggcccgga cctcaccggc tccggcaacc gctacacgct gcgcgacctg
    11101 atggagaaca tcatagaccc cagcaaggtc atctccgacc agtacgacag ccacgagatc
    11161 gtcaaaaaag acggcagcgt catcatcggc cgcattgtcg tcgaggagaa tgaaaaggtc
    11221 ttcatcatga ccaacccctt cgccccgaac gaccacatca gcgtggatga aaaagacatc
    11281 ctgcgcaaag gcacccgccc cgtctccatg atgccccccg gcctcatcaa cgccctcaac
    11341 ccggacgagc tgctcgacct caccgcctac cttctcagcg gcggcaaccc gcaggacaag
    11401 atgtttcaga agtagtcgcg cctgagtttg gtgggaaaat tacggtgccg ggtttcgagc
    11461 cgcctctgac ggatggatgg tttgaaagcc gcgcgccccg ccggcgttcc agcggctcgc
    11521 tgcttatgaa acaccccctg cttctcctca ccgccctcgt tttcacctcc gccatggccg
    11581 ccgacggccc cggcgagctg atgttttcgg acgacttcga gcgcggcgag tcgcaggaga
    11641 caaaggacga gccgggcaac ggctggggca ccaacagcaa aagccgcgcg aagggccaca
    11701 agcaggtgga cctcaaggac ggcgtgatgc gcatcttcat ccacgcggag gcggaccatg
    11761 cggtgtccgt gacgcacccg gcggagttca ccgacggcgc ggtgagcctg cgcttcatgc
    11821 tggaggacga gcgggacagc ctggggctga acttcgccga cctcgagttc aaggaggtgc
    11881 atgccggcca tctcttcgtc gccaaggtca gtccgaagtc cgtgcagcta caggatttga
    11941 agaccggcaa catgcggctc gacatccgcg aggcgcgcca ggcgaaacag aagctcagcg
    12001 ccgagcagga agccgccgtc aagggcaagg acaagatctt cccacatgct gtcgagaccg
    12061 ggaagtggca tgaactcgtc gtgacgatca gtggggacac gctctcagcc agcattgacg
    12121 gcaagcccgc cggcaccttc agctcccccg gcatcgcgca tcccaccaag cgcacgctgc
    12181 gcctcgccgt gccgcgcaac gcggtggtgg atgacgtgaa aatctggcgg aagaagtgag
    12241 cgcgatcagt caaacttcac gctcgtcacc cacgcggggc ctttgccgca gtcgtaggtt
    12301 ttgagcgtga tgagagtgcc ggtttcagca ttgcggcgga agacgatgag cttgtgcgaa
    12361 ccttctccgg cggagacgac gaatcgatca ccgggcgtga gacagaagga gcgcggcgtt
    12421 ttttcggtcg gtgtctggcc gtgtgcggtc agttcgccgg tttgtgcgtt cacggagaag
    12481 acggcgagac tgtcgtggcc gcgattcgag gcataaacga agcgtccatc agcgctgacg
    12541 tggatgtcgg cgcaggagcc tttgtcccgc cagtcctccg gcacagtggg cacgctctgg
    12601 cgggctttca gcctgccgtt ggcagcatcg taatcgcaca acgtcacgct tttgccctgt
    12661 tcgttgacga agtaggccca cttgccgttc ggatgaaact gcatgtgccg cggcccctcg
    12721 cccgcgccac cgggaagatg cggcggcctg ttgcgcgtga gcgtgccggc ctgcgcgtca
    12781 aagcggagct gctccacctt gttcaattcg cccacatgcg gcacaaaggc aaaacggttc
    12841 gccggatcga cctggatgca gtgcgctttc ttgcccgttt cgagtgtgac caccggcaga
    12901 ccgctcacgc ggccgttttg ggtcgccgat aaaccaacga cgccctcgct gtaagacgcc
    12961 gcgagcagcc agcggcctgt tttgtccgca aaaacatagg ccgcattgaa acccgcatcc
    13021 gcgaagctcg cattcgacag cgctccggtc ttcacatcga catccagcgt ggcaaactgc
    13081 ttcgccgagc gcaccgacgc ataaagatgc tgccggtccg gactgatcgc gagactcccc
    13141 ggcgctcccg gcagcgttgc atcgctcaag cgggtcaatt cgcccgatga ttcatccaac
    13201 gaccacaccg cgatgcgttt ctcaccgctt tccgagacgt aaacgatggt cggcccggct
    13261 aaggccgatg tgacgaggca gagaaaagtg aggatggctt tcatggagtt gggatcatgc
    13321 acaaacgatg cgcggaggcg ggagtcttca ccgttcctta tctcttcgat ggtcatgatt
    13381 gccgcactcc cgcagcgacc attccttctt catactttgg ttgggccgat cctgtccgac
    13441 tcccgcacgc caatttctgg cagtcgcctc ccaggtgtcc gaggcgcgtt taaccagcga
    13501 tcacttgatt ttagtattgt tcgtcgggcg gcttgtccgc tagaatcgtt ctgagtgtgg
    13561 agactggctg ttccaggcga ccccactgtg aaccgttcac gaaaggaaca ccatgaatac
    13621 gccggcactt ctttccgccc tcgcgaaaca cggtcttgga tggcctcgat acttcgccaa
    13681 ggcggcagtc tatctcacag gattcatcgg tctcttcgcg agcgctgagg tcatctcctc
    13741 cggctcccag gtggcgcggg cgctggccgg gcaggcggaa aaaggtctgc gcgtcacggc
    13801 cacggacgac ggtgaagcgc ggctgcactg tgcttttcaa aaaatagctg ctgaggcgac
    13861 tccggccggg ctgtggctta catcattgag tggcgcggtg cctgatcggt tccagatccg
    13921 cgctgatcgg gcgggacgtg gaggtgagtt tcacgagctt gaggagaagg gcggcgtgat
    13981 cgtgacggat caaaaagccg tctgggcgcg gacaggtctg atcgaggagt attccgtgag
    14041 cgtggacgga gtgcggcagg atttcctggt gaccgaacga cctgcggggg gcggtgcatt
    14101 gcaggtggag ctggccgcgc tgggctgccg ggccggcggc ggcggggatg gggcgctgct
    14161 cacgctggcc ggatcgggga ggcagatcgc gtatcatcgt ctgcgggtga cggatgccgc
    14221 ggggcggttg ctgcgggcgc ggatgacggc aaccgcgccg gatcgtctcg tgatcacggt
    14281 ggaggatgcg gaggcggtct atccggtgcg gattgacccc acttttgcgg acgcggactg
    14341 gaacagcctg ggcgggctgc cgggaaccaa tttcgtcgtg tatggggcgg cggtggattc
    14401 ggcgggccgg cttttcctag ggggtggctt cagcgtcgcc gggaccacgc aggcgcaaaa
    14461 catcgtgatg tgggacggca gcgcctggag cgcgctgggg gaggggctga acggcaccgt
    14521 ttactcggtg gcggtggact ccaatgacca cgtttatgcc acggggactt ttagccacac
    14581 cggagatggg gcgcttgttt tgaacggggt cggcaagtgg aacggcacgg cgtgggaggc
    14641 gctggggacc ggtctagggg ggactttccc acacggcagg gcgctggcgg tggatgactc
    14701 ggacgatgtc tatgtggggg ggctgttcac gacggcggga ggcgtgacct cgccggatat
    14761 tgcgaagtgg gacggcagcg ggtggtccgc cctgggcaat ggcagcaatg gaacgatcaa
    14821 tgccgtggcg gtggatgggg cggatgtcta tgtcggcggc actttctcgg ccatgggtgt
    14881 ggtcagcgcc aacaaggtgg cgcgctggga tggcagcgcg tggtcggcac tggggagcgg
    14941 tctgggcacg gtgaatcagg tggtgaactc cctggctgtc agcagcggcg gcacggttta
    15001 tgcggcgggc agtttcacct catcaggcgg cctgccggtg aacggcatcg cggagtggag
    15061 cggggcggtg tggaacgagg tgggcggcgg tctcgatggt ccggggacga cctgcgcttt
    15121 cgatacggcg ggcaatctgt acgtcggcgg cgcttttgaa acggcggggg ctgtcagcgc
    15181 caggaacatc gccaaatgga atctcctgac cacgacctgg accgcgctgg acactggcac
    15241 tgacaattca gtccaggtga tcgtgagtga cgcttcgggc ggcgtgtttg cgggcggcag
    15301 tctgacaacg gcgggatcca ctggggcgcg ctccatcgcg cactgggatg gtgcggcgtg
    15361 gagcgcgctg cacagcggca tcaacgacgg cgtgctggcc tttgcagcgg actcggcggg
    15421 gcgggtgtat gcggggggcg acttcgtgcg cgcgggcacc acgctggcgt cccatgtggc
    15481 gatgtgggac ggcacggcct ggaccgctct gggcaccggg gtgaatggca aagtgctggc
    15541 cctggcagtg gatgcgagtg acgatcttta cgtcgcgggg gagttcaccc tggcgggcgg
    15601 cgcgtccgcc aaccgggtgg cgaagtggga cggcagcgcg tggtccgcgc tggcagacgg
    15661 actggatgac cgtgtcagcg cgctggcgct ggacggcggc ggcaatctct acgccggggg
    15721 ttcttttctc aactcgggcg ccgcaccgat ggcccgcgtg gccatgtgga atggcacggg
    15781 ctggtccgcg ctgggcaccg gggccgcaag cggttctgtg aacgcgctgc tgtggtcctc
    15841 atcagacagc acgctgtacg cgggcggcgg tttcaccatc atgggcggtg tcacggtcaa
    15901 tcgcgcggcg cgctggaacg gcaccgcctg gagcgcgctc ggccagggct tcaacggcac
    15961 cgtgaacacg ctggcgctca gccccggcgg cgtcctccat gctggcggca ccttcaccca
    16021 gtccggcacg cagacgcgga accgtgtggc gcgctggacc ggcacgagct gggcggcgct
    16081 cggcagcggg aacggcggca cgatccacac gatgactttc gacatctccg gcacgctcta
    16141 tgtgggcggc agtttttcct cggctggcgg aggggccgcc aacaacatcg cgcattggaa
    16201 tggcgtgtcc tggtttgcca tcggctcggg cacgaatctg ccggtccacg cactgctggc
    16261 gcatccggcg ggccgtttgt atgccggggg ctacttcagc ctcgcgggtg acaaggtgtc
    16321 cggctacgcc gcctggctgg acatcgcggc gccggagatc gccgtcttca acggtgccag
    16381 caccgcgccc gccgatgagc ggaacgacgg cagcggcttc tgggactttg gcacgcaggc
    16441 gcgcgactcg gtctccgccc cgcagacgtt cacgatcaaa aacgtcggct cggccaccct
    16501 aagcggtctg gccgtggtcc tggatgagga ttgggatgat gaccattttt cgatcaccca
    16561 gcccgcgctc accagtctgc tgccggggga aagcaccacc ttcacgcttg ccttcgcccc
    16621 cctggcgctg ggggacttgt ttggcgtcgt ccagatcctc aacaatgacg aaaacgagtc
    16681 gcccttcctg atctccgcgt ccggcagcgg cgtgtggacc atccagcagt ggcggcagtt
    16741 ttactttggc accagcgata acagcggcaa cgcggccaac agcttcgatc ccaacttcga
    16801 cggcgtgccg aacgtggtcg cctacgcgct ggggctgcat cctttggaaa acagcgcgca
    16861 cctgctgcct gtggcggatc ctggcgactt ggaggctggc tggctgagtc ttttcgtgga
    16921 cgaaggaccc ggcgtcgtcg gcgtcactgt cggcgcggag acttccgaga ccttggaagt
    16981 cgaggactgg acgccagcaa cggagtacgg tgacggaccc taccattacc tctccgagca
    17041 gatcggcctg cgggacaggc tcttcatccg cctgaccatt gatgtgatgg aatgaatggc
    17101 tcgcgcggac cgctgccaac agcggatcag tctcaatcca acacggaaag gcgagaacat
    17161 agtcattgcc ccagatcatt cgcgcgcacc gcggcgagtt tggcggccac tttggccttc
    17221 cattcctcga tgcgtgaaac gtgatcgggt gacgaggcga ggttggtgag ctggcgcggg
    17281 tcgttgtgca tgtcgtagag ctcgatgcct ttggaggcgt cttcagtgta ttggatgaag
    17341 acccagcgct cgtcgcgcag catgaggccc ttgcttgatc cggcgacgct gaagacggtg
    17401 tcatgcaccc tggcctgcgg atcgtcgaga atggggctga tgtccttgcc ttgcagtcgc
    17461 gcaggcactt cgaggccgca gagcttggcg gtagtgggat agagatcgag cagttcgacg
    17521 agcgaatcgc tcacgccggg ctttttgccg ggcacgctga tgatgagcgg tgcgcgcgcg
    17581 gattcgtcgt gcaggctgac cttggcccag aagtcgtgct cgccgagatg gtagccgtgg
    17641 tcgctggtga agatgatgat ggtgttgtcg gcctggccgg acttttcgag cgcgtcgagc
    17701 accttgccga cctgcgcgtc catgaagctc accgcggcat agtaaccggc gagcgccttc
    17761 ttttgccgtg tgacgtccat ctgcatgttc acgctggtct tgtagttgat gccgggcttc
    17821 ggaatgtcgt cccagtcgcc gggcaccttc tccggcagca tcatttgctc ccacggatac
    17881 atgtcgaaat acttcttcgg cgcgacgaac ggcacatgcg gccgcacgaa gcccacgccg
    17941 aggaaaaacg gctccttcgc gtgctgcgcg atgagttcgc aggccttcgc ggccgttttg
    18001 ccgtcggagt gcaccaagtc atctccatcc gcggcgacga tgacgaaggt gttgccgccc
    18061 atgacgggct ttttgccatc cgggttcttt tccaacgtct cgccgatgcc gggtgcctgc
    18121 cactcgggtc cctggctgtt gaagcgctcc gtccaggatg cgggatcgtc cgcctcgtcg
    18181 ccacctttct cgatgccgcc gggcacgccc atgtggaaga tcttgctgac acgcgcggag
    18241 tagtagcccg cattcttgaa atgctgcgcc cacgtcgcgc gctcgccgat ggccgcgcgc
    18301 gggctgatgt agccctgcac gccggtggca tgcggatagt agccggacat gaacgacgcc
    18361 cgcgaaggcc cgcagtacgt cccctggcaa tgcgcccgcg tgaaccgcat cccgcgcgcc
    18421 gcgagacggt cgatgttcgg cgtctggcag accttgttgc cgtagcaacc gagcgcggtg
    18481 aacgtgaggt cgtccgagat gatgaagagg acgttcggct tcgccgacaa tgacgaaaga
    18541 tgaatggcga atgacgaaag gagaatgagg aggcgcttca tggagggaga agagagttgc
    18601 tgtcctttac actgatgcca gctctcatgg aaaagcctgc acgaccacga tttcctttcc
    18661 attggccacg gcaaatctct ggttttgcct gtcacgggca attgcaggcg ctttctccaa
    18721 atggtggatg atgctgctac tgtcctggcc ggatcgaatc tcaggccagc tcgcaatcaa
    18781 ccgacctgtc ggcagatgat aaacttttgg gtgttggtag aatgaaacca catgatcggt
    18841 tcccaatgcc atcatagttc ctgcggcgag ctcgatttcg aactcatgaa cggtttctcc
    18901 ctttgtaagc gagatggctc tgattctggc taccgtcttg tcaccttcgc cgtaggtaac
    18961 acgagatgag ttttcggaaa ccaggacggt ttcatcatct acaaacgcgg ctgagttcac
    19021 ttcgccccag tcagccagtc cgggaaatga gatggaatca agcgcgccgg gattcgagca
    19081 gacgccctca agattgaaca tggcgatggt gtcaaaaggg tgccaaaccc atccggcact
    19141 tatcaagcac tgcccattgg gggaggcttc aagacgcgag tggaaaaagt ccgctggctt
    19201 gcgcagtttg gactttacgg aatccagcac agaggcaccg gtctcgacct cctcgatctc
    19261 caaatgattg tagtcttcag gacagtgaac gagccattcc gtcttctgat tccccacgaa
    19321 agacaccggg tattcgaagg cttgtgaaca gtagtaggaa cggttgattt cacgcagcag
    19381 ttttggcagc tctccggcga gatcaataac cagccccttc gttcccaaac gctgacaaag
    19441 aaccgcatat cgtccactgg gtgaagtgac acacatgtca aacgctggcc caccccaaaa
    19501 cagttggttt ggcgtgattg tgccatcgag gtcgtaaagc tgtcctccag acgctaggtc
    19561 gatcaggcag tcacctagcc agttcagggt ttgaacctcg gacttggcat caattctcag
    19621 gagcgtaaag ttcatcgtaa ttcaatcaag ctactcacca agaacaccaa tctccgctgc
    19681 gctggcgagt tcgttgattt cgcggtgctc ggagaggatg cggagcagga cgtagcggcc
    19741 ggtggcaggc gtgcaggcga tggtttgcgg ggttttgatc ttttgcagcg acgttttgac
    19801 ggccgcggat ccgaaggcgc cgggactgtc gctgatggcg aattccacgt ccttcgccgc
    19861 gccgttccaa ccagcgtcct gacgtccgag atagacgaaa ccgcgaattt gatgcggcgc
    19921 gccgagatcg atgacgagct cgtgaggtgg tttcgcagcc ggtttgctga aacggctgtg
    19981 ccagagcgtg tcggggtcgc cgtcgatggc gttggcggcg aatttgctgt tgccggtgtt
    20041 ctcactgctc acatggacga ccttccactc tttggatgag agcatacctt tcaccgggtc
    20101 catcgcggtg gacttggcgc tcttcttttt gcctccgccg ggtgtggggt tgtagttgag
    20161 gtcatcctgc ttgccaaatt tcgcacggcg gtcgcgctca tggcggtcgg tgcggctgaa
    20221 ggtgccttcg cgcgcgggtt cgtgcgcctt ctccgccagc gcggtgagtt tcgcgagcac
    20281 atccggcttc gtagcagcga tgtcttttga ttcagcggga tcggtggcga ggtcgtagag
    20341 ctcccacgga gcggtgggct ttttccgcac cgcgcgccac ttgccctgac ggatcgcggt
    20401 ccagccgctg atttcccagt aaagatactc gtgctgcttt tgctccgcgt ttttgccgag
    20461 cagcgtgggc aggagggaaa ggccatcaat gtcggcgggt gcggtggctc cggtgaccgc
    20521 tgcgaccgtc gggaggatgt ccgggaagta gccgaggtgg tcggacacag cgcctggagc
    20581 gatcttcccc ggccagcggg cgatgaaggg gatgcgcagg ccgccctcgt agagagtgcc
    20641 cttggtgccg cgatactcga cgccactttg cgggtctttg ttgccgctgt gaacgccgcg
    20701 cgggtgctcg gccgacttga agtagtcgtt cgcgccgttg tcgccgctaa acatgaccag
    20761 cgttcgctcc tcgatgccga gctcttggag cagcgcgatg atctcgccga cctggcggtc
    20821 gatcatcgtc accatggcgg cgtagcgacg cgcgtcctcc ggccacggct tgtcttggta
    20881 gagcgcccaa gcgggatcgg tgtcggggat ttggaaattg ccgtgcggcg gcgtgtaggg
    20941 caggtaggcg aagaaaggct gttttgcgtg atcgcggata aacttcatgg ccgcgtcgtg
    21001 gatgacgtag tgcgagtagg ttttgccggt ggcgccttga ttgccttcca gcggcacttc
    21061 ctcgctgttt tgaatgagat acggcgggta gtagctgtgc gcgtgcacct ggtcgtagta
    21121 accgaggaag gtgtcgaagc cgtgcttctc cggcacgccg gtggaatcac gaccgccatt
    21181 gccccacttg ccaaaaccgc ccgtcgcata gccgagcggt ttcaaaatgg atgcgatggt
    21241 ggcctcgtcg gcccgcagcg gcgtgccgcc accattcgag cggacactgg tgtggccgct
    21301 gtgcttgccg gtgaggaagc agcaacgcgt gggcgcgcac acggacgagc cggcgagcag
    21361 gttgttgaag cgcatgcctt ccgccgccat gcggtcgaga ttcggcgtct ggatgttttt
    21421 tccgcccatg aaggccggct cgtagtagcc gagctcgtcc gccatcaggt agatgacgtt
    21481 cggcttcgcc ggcaatgacg aaaccagagc cgagcaaagc gagacagcca gcaacgcgaa
    21541 tcgtttgctg ccccacaggg gtgagcgtag cgaagcaatg aggaatgatg aagcgaaggc
    21601 gagtgttttc atggtgtgtg aggcatgtaa tccagttcaa ggaagtcctc gacctcggga
    21661 atccagcggt tttggacaaa ggcggtgtgg tcgggatggt tgttgtagaa ggcgtaagcg
    21721 ctctcgtcgg cgaactccat cgagagaccg aaggtgaaga gattcttcgg gctggtctgg
    21781 cgcaggcatt cgaagttccc cacgccttcg atgccggcga gagcgcaggc ggcgcggaga
    21841 aaatcgcgct cggcgccgga gccgggcgcg tgcttgaggc ggaaggtgac ggtgtggcgt
    21901 atcatgacag gagtgtcgca gtggggatgc gctgtagatc agcgcgggag ccgggcgtcg
    21961 agttcaccga gcttgaagcg cacgctgacg acataggcct cctcgccctc agtccagtgg
    22021 ccgtaggtgg tggtgacaaa ggtgccgtcg ggcagcagct cgactcctgg gtaggtggtg
    22081 tcctggcctt ttttgttgtc catgattcgg acgcgatact ggccttcggc gccattcacg
    22141 atgtcctcgt attttcccac ccagccaacc cagtcgccct tggtggggct gacgtgggtg
    22201 gtgtcgcgga agctgatgaa gagacggccg tcgggcgcgt atttcgcgac gtggcggtcg
    22261 ccggtgaggg cggcggggag ttctttcggg tgcgaccagg tgaggccctc gtcgtcgctg
    22321 aaaatgacga aggagttgta cttccggctg ttctcacgca gcaggacggc gatttgtttg
    22381 ccgtctggcg aacgaagcgc tccaggctcg cacaggctgg catcgggcag cattgcgatg
    22441 ggagcgggca cgctccaggt gaggccgccg tccttgctca aagtcgtgta aacccagaaa
    22501 cgccacggtt tggccgtgga ggacgagtaa tccggcgcgt gcgggctttt gacgcggtac
    22561 ttttccacct cgccgccgcg gatgaaacgc ccatcgtcat ggaagaaagc gaggtaatgg
    22621 ccgggcgtct tcaaggcgag cacggtggac atggtgacga cgccaccgaa atcaccgatg
    22681 gccttcaact cgctccagtt cgcgccatcg tcttctgaaa cggccatgcg gatgggataa
    22741 agaccgctaa acatgatgag gcgcttcttt cccgctgcat ccacgacgcg atgaagcgtg
    22801 ggcacttcca gcgaagtctc ccagttcttc ggtgtggcca ggcggtcgct ccaagtgagg
    22861 cccgcatcgt tgctgcgttt gtaaacgatg gcaccgcgtc cgtggccctt cggatagacc
    22921 gcgagcatcg tttggttgtc ttcgagcagg acagtcgtcg gatgtccgag atactggcct
    22981 ttctcgcgat ccacgatgac ctggcggttt gtctcgccgc tgatgtccac cactgggatg
    23041 gtgtagccgg gagcgagggt ggtcttgaga ttcttcggcg gcgcgagtgt gacatcggcc
    23101 ttttggagct tcgtttgcga gaagacggag gaggcgagga ggaggggaag gaagatgagg
    23161 cgcatggtgt gaggctaacg cggcccgtgc gcgtgttttt gcccgccggc tgcgcggacg
    23221 ctggacgagg cggcgtgccc ggcgctgcaa gtcaggcttt tttttcggcg atcgcgagca
    23281 cggtctggaa gccagggctg cgggattcct tgtggacgat gcctgtctcg acggaggcaa
    23341 agccggcggc ctcaagcatc ccgagcaact cggcctcgga gaagccgagc cacacatcgg
    23401 catagagttc gcgggctttt tcgaaggcgt gtttgagcag gtcgagcacg gcgatgcggc
    23461 cgccgggctt gagcaggcgg tgggcggcgc gcagggcttt ctgcgggttg gcggcgtgat
    23521 gcagggcctg actgaggatg acgaggtcca cactggcggg gtcaatgggc ggatcatcga
    23581 ggtcgccgag gcggtattcg agattagcga agccgtgctc gcgggcgagg gcggaaccgt
    23641 aggcgaccat tttttcagaa ctgtccacgg cgatgacccg gcgggcgcgc tgggccagga
    23701 gctgggacag agtgccctcg ccggcgccga ggtcggcgat gacgagcggc ggcatgagtt
    23761 tgagcagggt ctcgccgagg gctttccagg agcgtccggg gatgtaatgg cggccgaatt
    23821 ttccagccag ggcgtcgaag taggcctggg cggcgcgggc gcgttttttg agggacacct
    23881 tgagcgcggt ggcgtccttt ttcacctcgg gcatggaggc gccggcggcc tccagaaggg
    23941 ccagaaagtg ctcgcgagcg ggagcctcgc gcgccggggg cggcgccagg ctgtagagtc
    24001 tgttcttgcc gctgcggcgg tcgctgacca ggccggcggc cttgaggcgg gagagctggg
    24061 cggagatgtt cgactggggc agggccagga tttcctgaag ctcggccacg ctgagctcct
    24121 cctggcgcag gagcaggaga aggcgggcgc gcgtggggtc ggcggcgagg ccgagtgtct
    24181 tgagcaggga ggccatgggc gcggcatgtt gggacgcggg cggcggcttg tcatgcggca
    24241 agttgcgcgc gggccggatt ccgccttgtc tggccgcagg ggagcgctac catccgcgcc
    24301 cgcgcctgcc gtttccgcaa ggcgcgcccc cctcagccag catggtgaaa cgcgcgctca
    24361 tcgcctgtgt cctcgctctc ggcgctccgg cccggccggc agcggcccag gaggcgcgcg
    24421 cgccggagcc gcagacgctg gaactgatgc ggctggcctt tcaaaaacgg ctgatggagg
    24481 ccgcgctcaa cccgctcaag cagcagctca ccagcctggc agaactggaa aaacagagag
    24541 ccgcagccgg cgactatgcc gccgccatcg aggcgcgcga ccaacgcaaa cgcctgacca
    24601 cggagctgga gcggctggac aaggagcttc tgctggcgca gacgcgcgag caatccctca
    24661 aggcgcgcct gctgccggag cgcgtttccc tggatctggc gcacgccgaa ctggacggcg
    24721 gagtgcgcat ggatggcggc gcgctcaccg gttggagccg gcccggcgcc gcggcgaagt
    24781 ggaaactgcc ggacctgcct cccggaggct acgaggtggt cgtccgctac cgttgcggcc
    24841 cgctggaggg aggctcgctg caggtggccg aggcgcgctt cactctcgac gcggatgtgg
    24901 tgaccaccct gcgcggcccc caggaaaaaa atctcggcac actcaaggtc accgacggct
    24961 ccggcccgct cgtcatcagc gcgcgctccg tggtgaagga caacctgatg cacctgctgg
    25021 ccgtgcatct cacccccgcg ggacgctgac cccatgaaga taaccgcgcc catttttctc
    25081 gccatcctct cctgctcctc cgcgcagacg acagaccccg cgccgctggc ctcggcgcgc
    25141 gcggccttcc tgcgcgatgt gctggcctcc tccaaaacac tcagcggcca gtatgagtcc
    25201 gcgctggccc gcgccgaggc cgagctggcc gccgccgcag agtacgagga ggcgctgctg
    25261 gtgcgccgcc ggcgtgacga gttgaaggcg ctctatcccg tgaccggggc ggagatcgcc
    25321 gcctccctgg ccataccgct gcccgcgcgc caggcccgcc tttccggctc cgtggaggcg
    25381 cgcggcgagg agctgaccca atggcgcacc aatggcagct ccgccgagtg gacgggtgtc
    25441 aaggtctcac ccggcagata ctacctggag ttggaggcct gcctcattga gacagccccg
    25501 ccgccggatg gcatgctgcc ggggcttttc cgcccgcagg agcatgtgac ctttgaattt
    25561 cacgagcttt ccctgctgcc cggcgcggcg gaaaatcgcc gctctttcga gatcgccccc
    25621 gcgcgcgcgg acggcgcctt cacctccctc caggccgggc ctgtcacttt cacgcgcagc
    25681 cccgtgagcc tgcgcctgac agcggccgcc ggctacccgg ccaggcacat ccgcctgcgc
    25741 gccctgcgcc tcattcccgc cgcccaggac gcccccacca ttgtctcgcc ggacagctcc
    25801 acccccacgc tagacgacct gcgcaagtcc ctctccgaga ctttggccgc cgtgcagaaa
    25861 cccattgtgg acggctatct gcaaaacctt cgcgacctgg ccgcaggcac gcccgcgctc
    25921 aaggacgccg cagacgccga ggcgcgccgc atcatccgca tgctgcagga caacggacgc
    25981 tccacgcagc ggctgctcgg cgcggccacg cccttgagcg ccttcgacga ccttgatggc
    26041 gcggttttcg ttcccgaccc cgccaacgag ggcgaccgct ttttcgtcga gcacgacggc
    26101 agccggctcc gcgtccagct tctctgggtc gaatgcgcgc cggtggatgc cgcccgtgac
    26161 gcggaccgcg ccgggcgcat ccgcgagcat ttccgcatcg aggacaccga ctcgcccgcg
    26221 cgcctcgcgc gggagttcac cgcgggctac ctggaaggga agcccctccg cctgctcgtc
    26281 cgcccgggca aaaacaaaga cggcagcatc tccgcgctcg tctttctgcc cgaagtcggc
    26341 ctttaccaaa acatccttgt ggaccagggc ctggccgccg tcctcgcgcc cagggaaaag
    26401 cgcggagtca gcgagaccgg cctgcacgac tccctgctgg cccgcgagcg cgccgcccag
    26461 cgccgcaaac ccgcgcccgg agcctgggcg ctctcggagg gcgcgcgccg atgaaaaccc
    26521 cttccccatc cgtcatgcgg cggctttttt ccgcaaccct cgcgctgctc atcgcctttg
    26581 aggcaggcgc gcaaaccccg gcaacccatt ccaccccggc cccctccagc ggcgaggaca
    26641 cgcgcctgcg ccagatggag gccatctacg tccagcagct ccgctccctg catgtgcccc
    26701 tgctcggaaa atacgtcacc gagctccagc gcctcgccgc tcaagccgcc gatccggctc
    26761 cttatcagcg cgagatcacc cgcatccagg acatcatcag ctccggcggt gtcgtggacc
    26821 tggccgacgc cgcgcggatg ctcaaagccc ccgccgagcc gccc
//
LOCUS       DAOMYJ010000048        26528 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Prosthecobacter sp. isolate AMR_MDS_5629
            NODE_668_length_26528_cov_27.577872, whole genome shotgun sequence.
ACCESSION   DAOMYJ010000048 DAOMYJ010000000
VERSION     DAOMYJ010000048.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867850
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prosthecobacter sp. (wastewater metagenome)
  ORGANISM  Prosthecobacter sp.
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Verrucomicrobiaceae; Prosthecobacter.
REFERENCE   1  (bases 1 to 26528)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 26528)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.30
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:34:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,742
            CDSs (total)                      :: 4,684
            Genes (coding)                    :: 4,676
            CDSs (with protein)               :: 4,676
            Genes (RNA)                       :: 58
            tRNAs                             :: 55
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26528
                     /organism="Prosthecobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_668_length_26528_cov_27.577872"
                     /isolate="AMR_MDS_5629"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1965333"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            246..13010
                     /locus_tag="PLP58_07170"
     CDS             246..13010
                     /locus_tag="PLP58_07170"
                     /inference="COORDINATES: protein motif:HMM:NF024353.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter-associated beta strand
                     repeat-containing protein"
                     /protein_id="HRJ08332.1"
                     /translation="MKIKEKNCRQNSFLTSKTWGLHVALVLSVLPFMAPAQTEWLPFT
                     GDVHSWQTTANWSTGVIPSGMSESVLFGFSPYQNQVITLDADVTLGSLIFANQAKSFL
                     LSSNTLTMDNGDNGSLLSNTGAATNVITSNIVFADGVSNTVAVGDGRLVLTGIISGSN
                     ELTKTGDGMLILAGAVSNTYTGVITLNGGITLARPQGNNLLMLGATGAGTVVNSGATF
                     ASGNDFSGGAGLQTTEGWGTINEAFNITGDGYLRQGAIRKMTGREQDTIGGTVTLDGN
                     ARLHADYGTLALSGAVAIGANTLTATGAGGFVTLGSSSVAGSGDIVHYGLAGFRLQGN
                     SNTYTGTIKSNLGEIRADTGDTTTGNNPYDTIFALDLRNSLLRLNYPNTAGTAPNVAD
                     SRFSTTAPISMRSSSINIDNASFSGTSTTFFDYAVAQTFGTTTMHSGANYIYFRSADA
                     GSVTMTFTDLLNPNPGTTLEFQIDSLTGGAAHNLGVTGSKHRILNTALEGGAGVPFLG
                     GWARTSGGQSTTSLMEFVKYVVGSGYTMLASGDYATNTAEGTWASGQNVKITTTATTT
                     LTADRTVQSLNLQSTTARTLTGNPGTTLEIESGGLISQGATHVISVPFLTAGAGGDHV
                     LYLHTPSSNIIRSIITDNGGNPVSLVKDDGGTVSFLGNNTYTGTTFLNEGMFRDIIGA
                     RNLTALGSGNFNMAGGAIGQSAYETDRDFTRALGTSVGEVQLTGGGGGGGGSVGFSAY
                     GAPIDINFGGSGATVVWGSPTFNPGIFTLNGGNATHVATLVNDFDLGGEQRYIRLDGG
                     SSAGNRAVMGTISGDITNGGIVKRGGGVLFFEDAKSYTDSTIIHEGELWLRNGTGTAG
                     ANVIGNDIMIGAASRLKIDAPSSIGSRQSIIMQNQDDNSASAIAFGPGYGNGADIRFH
                     SIISANGSSIPQTGAYDITLANQQRGNDRRNRVAVQLSGNHVFTTDVMAQIKAVAPDV
                     QAWLGADTGNGTYAGSTIPATGRTKTGSIEAFRLGSGGGTLTIANENVLDGAFPLIVG
                     AEDQTGRTNIGGVVFLPKAQNYSGIISQTDGTPLDAGNLIGAGGILVVGQNGALNSGN
                     DNISLRAGELRLGVDSANSFLGGVDSQYAARNVITKSANSVLRLQPITGASNGVLKLN
                     QFLMRMDAADRTFSLNSIGTHYTQTVFDGDGVLENGATARSAFFNVGNDNSFQSGVGV
                     LVFNGALSQTGTGAVSLQKRQGGVLVLAGDNTYNGTTNVQQGRLVLAHTGAAGNAGTT
                     INMNTNNDRRSDLEFRLDGAGPFVFDNVINTTGGNDGSTRVITVGSRTGASTDQSVQI
                     PTLTIGHAGAFTVNGTGSSAVFLDGFNGYQFEITGTLNLNRDIVLRTRGAFTTISGVV
                     AGAAANDLEKSEQGTLWLSGDNTYAGNTTVSNGYLVLGHDNALGTASTDVVFRNTAFT
                     QILASGARTISRNFINTATGSTQTLGGLDAGAKTFSGNINLSTRGISLTAAPGGDTTF
                     TGTVSGAQGVTKTGDGNVVLAPASGTGNTYTGVTSVSSGVLVGLAQAVSGSPFGENNA
                     FNVANGTLALQGGGGATTTSTTGALTVDGGNATVAVNAPAGATTFTFGSLSRANNATL
                     VLKGGSTNLGGATTEMVSFTAAPTLTNGIIGPWAVIGGPGLSDNSGHYAGFTGGNVVT
                     ATYSGTGDLDGLTSASDVWDAGATASTLTGNRSAHAFRASADVALGGNTLFLGFSGQA
                     GIILNNGADISGGTLSLGTNVLSIYTDDAAVSTLGATVTNFRGNTNNTLSTGLIKFGP
                     GNLEISAQQSFQGNVQVNQGTLSFSAANLAPTFENLNAVIGSEVVIRPGATVSLNGFD
                     QEFGNLSGSVVVSPVLNTAGTLELGAATLTVGRNTGDRTFSGQITGGAGSRLVKVGGS
                     RLILDNWDPARPNSLGTLEVLQGPVQSRLNDNSWAQAIGFANSIPASTEVLLRGGEFE
                     AYVNGDSTSNFQFIPLGHSISARDGNSVLDTNRWQGGGTNKILGFNNLTVDKNILTIT
                     GGNAVYPRFDGTFTMTANSRLQIDAPLLINGSITGHYALTKTGGSNLEIGGSNNGWDG
                     GTVLHDGLLLFGSRVPEVSERYMAGTNLFSYSATANLGTRDIVVNRSTAARFTAPSNV
                     LKDDGQRVLLFGSDGAGLPRVDIGVDAPLTDYSLRSTSKGALTIGLNDGFYTHAIDQS
                     LIGDGRWGLGAWATTFYTASTLGYGVDNLYRFAGVNGVLGITQSHALSGPAGLQVGTA
                     QVTQGFAINSGNGQARLYGDQGYTGNTLVFRNREAGSVQNFLEILGDSASPVFDVYGR
                     LMARGAGRFTNDAGVQVNTVNLHPGSILRLDYAMDVNDTIMISRLENSNLGLESTENK
                     WGDSTPMLLNGATLNLVSSSARVNRETIGVLSVKGGAAVLLERNGTNGQIILETPSIT
                     RVGQATFDVRENADELGRVDLQSQKFFINNGSSMEDAQGLLPVWMINPTRNTFLTYNE
                     DLGVQNAAFTSSTTTAGTGAAFLGGLTSASVASYGVGTGDATLAGTVDVHALRISHEA
                     ASNDTVLTGGQINIHSGGLIVDNRDNARVNFDTTNVFFGDGTTPVEGIIYSDQNNITT
                     RMGGVVTAANLTMHGAGNLQLTNTANAISGNIQMNGGRLFLDGVGTAGTGASITLTGN
                     YLQNNDGNQMPELLLRTLNANGAWTNGIIVAEHTPYVRVLGQSLDGTSLTTVRNQTIP
                     SLMIEGTNTQQGTTFIFGTTSNTNNANNYNLIVSGATTLGGSAPIGFRIERGGTNIQG
                     GGGFMTLAGAVTGTAPVIKSGDGYLVLQADNTGLSSPVTLNRGIIRGQGNNANNFFGT
                     GDYTLNFGTVEILHNNAGTYFNASGQDIFVNGAASINSNRNGGSNAANYTIGVNNGDN
                     TIVTSNGASLRLNAASFGDDFYLESRLIINDSAAWFQDNTEVWFRDQLVGGGRLTRTG
                     IWHTYFDNTVPNDNWSGTLDLQAGTVRVLRSETTLGGVGSSIILNPAAQLSVRTVANL
                     GTGNGITQLRTTSATSLPVLGIALGTEFTALRAHYNGLTATGTRNGVLAMDAGQSVSV
                     DPDMANFQNGNWFFGGATGSGTLAANSVAPWGPGGNKFLIGGGSGTITLSPTAGSAQF
                     AGSNQMIIGSALNVFGYSTVVFNANGNNTYDGGTLVTVSRNMDGGYRGMALSIQGGRA
                     TDTTYRTPLGSGLVDVFGEVRIEQANGTARNADNANANAWVFHPGSRIRFDNDAPFAT
                     AGTEGRWADNVPIALNTTVLEMFGDGATHPYNTETVGAISVAGGSEVVVRRRGAFLAE
                     IIAGDLTRVGNGTLMVTGMDNTTNTISGLGSTAATTAMRLLVANGASLMNNGMVDPWI
                     IGRIGGHFLRYDAAGGFQPLTTANTPNYIVSTATTIDGAVLPLNDGTEILGLEATGAV
                     TLGANLDVHALRVSRQINASINKSADGSFSSITIRSGGLNFADTGGTNRSPTINADLF
                     FGLNGTGDAYVFSDGTTGQINGKIHASNFIKSGPAEVYIRSNQPQFTGNWIVNGGALR
                     FTTPGGQSSGEVILNGSRMNDRDNTFNLTEVRYNFNSGSPDLFTWTGGKITGYNLNRI
                     YAITANDRLQQIPAVDLRTTNTVAGTGQEGLMIFLLDGARSTVRTGTVTLHDHYQVQV
                     ESNTYGTGSTSGFQFGSGTGVGGLNNQGLFDLRKVGDGVLTLGDNSLSFTGSRSITIG
                     EGAVRVLHGGAFGGAGNTGNIEQGGALEIAVAGWSPSATLVQQPGSMERWAVDGARIG
                     DVTLSSGVHWQIMHNQTGTQTVTLDGGSIMGYTPRDWDHVGVIHSLGSGVTIHLASNS
                     FLGQPFVSSNNSIWDQARIYDIGKINQTTASNPNDPGLRGSYLQIDGVITGPGGLTKL
                     GQDMIVLNGANTYAGATSVENGILQIGRNNALPVGTDLSTETSSGMFDLNGYNQEVAS
                     LSGDHGSVNNGAFDLNTLTLNQSASTTYGGQINGNVSVVKSNTGSLTLTSASGMRGDL
                     AISGGSVILGAAGSVNEARSIQIGAGSVFDVSAVTGGVYSYDGRISGGGVGALRADNA
                     SRAQIIGSLIVTDNIGAIARQGSISPGNSAGHLYVSGDLTLGGGLWGTSTKTERLTLE
                     LGAPTSTLAALGWDGSNVADWLVNVSPDVLNGLAGNLSGHDYVNVGGELTLNEHGGIG
                     VTLINGYQPQYGDVFNLLDWTSVSVGGFDAGPTPRSGGELGYDLNLPDLTAFQLTWHT
                     DLFADYGVIFVVPEPGRMMLLFFGLTGLFLRRRR"
     gene            complement(13062..14603)
                     /locus_tag="PLP58_07175"
     CDS             complement(13062..14603)
                     /locus_tag="PLP58_07175"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HRJ08333.1"
                     /translation="MRRTEHNQSEVKKTSGNAAHAAGEKRELEARRAAAHYRGLFENN
                     TAGVVFVDRGMVITGVNRTFVKLVGRKKDALVGAGIIELCSVPTRGLVSGQLQEAARN
                     TRRRTTLEMELVTKRGEILCTQVSPTFFMDEAGGLDGGVMVMTDVTAQKQALQDLWEK
                     NQFYEALIRDASVMIVILDENGVVTGMNRAAERISGFSAAEVSGQELWRLFHLDPSQA
                     DLVRERMEQLISGEVERVADPFRFIRKDGSVCHAETQTSLLRRPDGSPGGFVTTAVDV
                     TDRMRLESELVRIVEDEQTRIGNDLHDGLGQMLTGAATLLEMLVAGLEGQQKKDGARV
                     YEILRETVHEARRISHGLSPAAVKNRGLAGGLKLLAEHVNAAGQIHCATDIDSSVRVS
                     DPAQETHIYRIAQEAVSNAVRHSRTDLVKITLRRHEGGCILEVRDRGIGFDPSHPKSD
                     GIGLRVMRHRANIIGANLSIDSAPGWETVVRCRFRPSEKDSLRQNDKRPAKSAPQRRM
                     RKAQK"
     gene            complement(14600..15166)
                     /gene="def"
                     /locus_tag="PLP58_07180"
     CDS             complement(14600..15166)
                     /gene="def"
                     /locus_tag="PLP58_07180"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009957923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HRJ08334.1"
                     /translation="MPLKIVRYPDPVLRAKCRPVTEVTPEVRRLAAEMIETMRAANGV
                     GLAAPQVGQDVQLAVIDVSHNPKCVSFLRVNGEPADMSARMPVIFLNPVLEPGKDKET
                     DEEGCLSFPRLRGDIRRSASLKVTFQTLDGATQTWETDGLLARAFQHETDHLNGVLFI
                     DRMSAAAKVGLKRKLARLMEEWAEDDEA"
     gene            15282..18005
                     /locus_tag="PLP58_07185"
     CDS             15282..18005
                     /locus_tag="PLP58_07185"
                     /inference="COORDINATES: protein motif:HMM:NF024753.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HRJ08335.1"
                     /translation="MHRLIGLFLAASTIHAADWPVWRYDAGRTAFSPQAMPENPALLW
                     SRELPPLRPAYKDPRLQFDGGYEPVCAGGRLILGSSREDCVMAYDAETGAELWRVLAG
                     GPVRCAPVIVDDLVIFGADDGVVRCVRVEDGAQVWTKRAVPSGRMLLGNQRLISAWPV
                     RGGPVAEDGRVYFAAGVWPLEGVFVFCWDAATGDEIWCNDRCSYLYGIHPHDTQAMGG
                     LAPQGYLLVDGGDLVVPCSNAYPARLDLKTGALKQFELPSAGRLTGGWFASTPAEKKA
                     FRLQRWGLLIDSGVNAKRHEDKPRAEGIAGIRRSLHAAGREAGFDSLKLPGLAGRVHS
                     AIVADEKCFVVTEDGILHAYGTLQGEARHWKREISVQTAGEALAKKCLAAAGTERGYA
                     LMIGPNEPGLIESLILNSHLHLIILATDAAAHYRLNEAGLYGERASVVDIHAVAQPGA
                     LPPYFADVIFALEGGQEDFLHTLRPGSGRVIGPGASVIHARGPLEGASDYFADWQPSQ
                     DALVRAPLGVLWFDDALSHFKRSPQPKIIGGVMITSDKDWLDASNRKGKVDYRLQAPV
                     FSDIYTGRVLDEYEAPELRAKHGTVDMESIQPAQYRPPTQKDDWKPGAPQPGMRISPL
                     TGLEEPRVFPKSYGCDGGFDYGGIYTFRSGTAAFYDKKIESGTIHISGPRSGCTNSVV
                     PAGGILNVPYYYEGCTCSYPLPVALALTSMDERFEQWATWGSQSKDELRGKIERIGIN
                     LGAPGDRNTRDGTLWLDHPNVGGPSPEIEVENEPALPEIFYRHSIWVEGGEGWPWVAA
                     SGMKNLRRLTLRGLKPGSYLVRLTFACPDGEARVLRVSVQGRIVSERLELPRPMTART
                     QTHPAIPIRDGSLTLELEALEGGTLLSGIEVIREGLGIGNVPENARAPGRL"
     gene            18005..18715
                     /locus_tag="PLP58_07190"
     CDS             18005..18715
                     /locus_tag="PLP58_07190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ08336.1"
                     /translation="MNSPLRIFTAAACLLLPATLTRAHQVPSITVEAIFAADHTYTLR
                     VNLDPRVFLSAQPASLPPVEAAWYLEQSEEEKKQTHLMAWDYLKKNFQISFSGRLADF
                     PEYVFTAIDGATLDPLQPATPETHLLAAAKSRVPDEAGDFVLAFGPEANVSLILLIGE
                     EGRAGHRPQGLFPGETSRPHKIPQATAAVEGDAGETVSSTESSGSQAIAMAGLILLVL
                     LLFTPAIRRRIASGAQTK"
     gene            complement(18767..18843)
                     /locus_tag="PLP58_07195"
     tRNA            complement(18767..18843)
                     /locus_tag="PLP58_07195"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(18807..18809),aa:Arg,seq:acg)
     gene            19050..19571
                     /locus_tag="PLP58_07200"
     CDS             19050..19571
                     /locus_tag="PLP58_07200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018968949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HRJ08337.1"
                     /translation="MSAALPNWEKARDEFVAQWGALGSHWGINRTMAQIHALLMTAPE
                     PMHTDEIMERLRVSRGNANTNLRELVGWGLIRVVLKKGERREYFEAEKDVWQMFITIA
                     KERKRRELDPALMVLRSCAEQTQSEKTGAGLDFHRQMRELQEFVEFGMKVSDTVASMK
                     HASALQWAMRLLG"
     gene            19699..20598
                     /locus_tag="PLP58_07205"
     CDS             19699..20598
                     /locus_tag="PLP58_07205"
                     /EC_number="1.14.19.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016717 - oxidoreductase activity, acting
                     on paired donors, with oxidation of a pair of donors
                     resulting in the reduction of molecular oxygen to two
                     molecules of water [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA desaturase"
                     /protein_id="HRJ08338.1"
                     /translation="MTNPLRGKIVWSPVKSLWFTGHALVALAGGWLTAGVWPVAVCCA
                     LTVLTLCLGHTLGLHRLLIHRSFSCPIWVERVLVHLGVVVGMGGPFRMLLLHDIRDWA
                     QRQPRSHPFYTNQAHWWLDWFWQMHCELRLENPPVFKIEPAVRNDPFLRFMERHWMLQ
                     QVPWAVLCHALAGVPGLVWGVSVRIVVSLTGHWLIGWLAHNVGRREWHMEGHSVQGFN
                     LPRLGLLTMGECWHNNHHAFPGSARLGLAAGQHDPGWWALRMLMAAGLAWEAKTPATL
                     PDRPERKRLPAAPKSRATTFFTL"
     gene            20624..21019
                     /locus_tag="PLP58_07210"
     CDS             20624..21019
                     /locus_tag="PLP58_07210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007416950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ08339.1"
                     /translation="MNHAIIIHYTLYLALALCMTVWVARTLHKNGRVFLVECFHGDTH
                     LADSVNHLLVVGFYLVNIGFICLFLRAAGEVDGARGVFEMLSGKMGVVMLVLGAMHFM
                     NLLLLARMRRRARLAAMPPPVPPVLRVSA"
     gene            21042..21488
                     /locus_tag="PLP58_07215"
     CDS             21042..21488
                     /locus_tag="PLP58_07215"
                     /inference="COORDINATES: protein motif:HMM:NF016056.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DCC1-like thiol-disulfide oxidoreductase family
                     protein"
                     /protein_id="HRJ08340.1"
                     /translation="MHTLTIFYDARCGLCSQVRHWLLAQPAHVRLEFLPYDSPEAARR
                     LPGLRHLRADREIVVLADSGEVWQGAGAWIMCLWALREYRSWSARLAAPAMQAAARRV
                     VEWISRHRVGLSRLLRMKSDADLLSAAAQAPEAPACAVRHDLDLID"
     gene            complement(21478..22482)
                     /locus_tag="PLP58_07220"
     CDS             complement(21478..22482)
                     /locus_tag="PLP58_07220"
                     /EC_number="4.3.1.19"
                     /inference="COORDINATES: protein motif:HMM:NF004771.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine dehydratase"
                     /protein_id="HRJ08341.1"
                     /translation="MEPALPTLEEIESAAALIAPHVPPTPAHTWPLLSEMAGAEVWVK
                     HENHTPVGAFKVRGGIVYLDELLRREPGVRGVIAASTGNHGQSIAWSARRLGLRAVIV
                     VPHGNNPDKNRAMRTLGAELVEHGREFQEALEHSRTLAEAQGLHAVPSFHPWLVRGVA
                     TYALEFLRSVPDMEAVYVPIGLGSGICGMAAAKAALGLKTRLIGVVSAHAPAYALSFA
                     SGSLRTQTSATRVAEGVACSTPNAGALALIRRHVDEIVTVNDDECLFAMRELLENTHN
                     LVEGAGALSWAALKKASPVRHAKRLGCVMSGGNAGMELVMKSLASARKQAFKPVEFSQ
                     "
     gene            complement(22498..25596)
                     /locus_tag="PLP58_07225"
     CDS             complement(22498..25596)
                     /locus_tag="PLP58_07225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007416211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PSD1 and planctomycete cytochrome C
                     domain-containing protein"
                     /protein_id="HRJ08342.1"
                     /translation="MRSAPATTTLLLLLAASAHAAGKLDFNRDIRPILSDNCFACHGF
                     DEKKRKADLRLDTAEGAYAVLDGVAVIKPGDPAQSSVMQRIVTTDPDDIMPPPESHKK
                     LTPAQIGTLKKWIEQGAEYQQHWSFITPRKPAVPEVRNSKFEIRNSVDAFIQARLAQE
                     GLEPAPEASKEALIRRVTLDLTGLPPTPAEVDAFLTDTSADAYERVVERLLKSARYGE
                     HMARYWLDAARYADTHGLHLDNERSMWPYRDWVVRAFNDNLSFADFTRWQIAGDLLPN
                     ATMEQQVASGFNRCNVTTSEGGSINEEFVFRYAVDRTETTVAVWMGLTAGCAVCHDHK
                     FDPISQKEFYSLYSFFNSAADPAMDGNILLTPPILRLSTDEQKSQLAKLDADIAAKQQ
                     AIREAIAAIQYTDPAELTPPPPAQTSEVVWFDDDFPAKAKVEHAGAPTKFVTAEDGQV
                     FSGKRALTRTAEQVAQDFFSGGASFEVPANGVISVQCFIDPANPPSAVMLQFHTAGWN
                     HRATWGEEGAIPFGKVRTPENVKVGALPEAGKWMKLQVNAERLALKPGMKVNGFAFTQ
                     YGGTVTWDRLAMNSRVEPAKDPQWSWKVWTKNNQGKALSFLETELRNLVRGKKPEEWS
                     AEETAKLKAWWFENEYQGAREIVDGPRAEKLALEAKKKALEETIPATFVMADLPEPRE
                     SFVMERGLYDKPGEKVARTTPAAFPPMKTKAPQPTRLDLADWLLAPEHPLTARVTVNR
                     FWQQFFGTGIVKTSNDFGSQGEPPSHPELLDWLAVTFRESGWDMKALVRLIVNSHTYR
                     QSTRFTPESAAKDPQNRLLARGPRFRADAEVIRDSILYISGLLSLKVGGKGVRPYQPE
                     NIWEPVAFGGSNTRFYVQDKGESLYRRSLYTFYKRTAPPPAMTNFDAPNRESACLIRE
                     RSNTPLQALNLMNDVQYVEAARHFAQQLLKIPGSTDDSRITHAFRFATCRHPTAEEAE
                     LIRQTLEQHRAAYRARVEDAKQLISQGESKPDDSLDPAELAAWTMIANLVLNLDELVT
                     KG"
     gene            complement(25630..26319)
                     /locus_tag="PLP58_07230"
     CDS             complement(25630..26319)
                     /locus_tag="PLP58_07230"
                     /inference="COORDINATES: protein motif:HMM:NF013073.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NlpC/P60 family protein"
                     /protein_id="HRJ08343.1"
                     /translation="MISSPRIPIIGFFVLAACAAGAEPDKGARVSTLEVTELRDFDKN
                     PEPVRELIRAALALTRMNLTYTFASHDPGRGGMDCSGTIYHLLHSRGVTEAPRQSDQM
                     CQWVMDKGAYQRAEKAESLEDAVFAKLAPGDLLFWSGTYESTKRALPVTHVMLFLGHR
                     KGDGKPVIFGASDGRSYEGQKRRGVSVFDFRLPKPGGAAALHGFGAVPGLVREEIRKP
                     LLPWLPPFLKR"
BASE COUNT         4941 a         8692 c         7993 g         4902 t
ORIGIN      
        1 cctttgccat ccgctgggtt gagctgcgca agctcgacat caaagagccg gcgcaggccc
       61 gtggctgaca gttcgttttg cgggattcgc ctgagcgggg cggcccttgc ttcagacaca
      121 accaatttac gatgctttac agcgtgaaag gcaaaccttt atgcgatggc ggccagagcc
      181 ttaccagaga gcaagccatc gtctgataga cccattttcg cgaccccgct caaaatgcgg
      241 gccgcatgaa aataaaagaa aaaaattgcc gccaaaattc tttcctcacc agcaagacct
      301 ggggactcca tgtggcgctg gtgctttcgg tcctgccttt catggcgccg gctcagacgg
      361 aatggctgcc attcacaggc gatgtgcact catggcagac caccgcaaat tggagcactg
      421 gcgtcatccc cagcggcatg agcgagtctg tgttgtttgg tttctcccca tatcaaaacc
      481 aagtcatcac cctggacgct gatgtgactt tgggctcgtt gatttttgcc aaccaggcga
      541 agtccttttt gctgtcctcc aacacgctga ccatggacaa cggcgacaac ggttccctgc
      601 tctccaacac tggagccgcc accaatgtga tcacctccaa cattgtcttc gccgacggag
      661 tctccaacac agtggcagtg ggtgatggcc ggctggtgct caccggcatc atcagcggaa
      721 gcaacgagtt gaccaagacc ggggacggca tgctgatcct cgcgggcgca gtgtcgaaca
      781 cttacacggg tgtgatcacc ttgaacgggg gaatcaccct ggcccgtccg caggggaaca
      841 acctcctgat gctgggcgcc accggggcgg gcaccgtcgt caactcgggt gcgacattcg
      901 caagcggcaa cgacttttcg ggcggtgccg ggcttcagac cacggaggga tggggaacca
      961 tcaacgaagc cttcaacatc acaggggacg gttatctcag gcagggtgcg atccgcaaga
     1021 tgacgggtcg cgagcaggat acgatcggcg gcacggtcac cttggatggc aatgcgcgcc
     1081 tccatgcgga ctatggcacg ctggcgcttt ccggggccgt ggcgattggc gctaacacac
     1141 tgactgcaac cggggccggc ggttttgtta cgctgggcag cagcagtgtc gcgggttcgg
     1201 gagacatcgt gcactacggc ctcgcgggtt tccgccttca gggaaacagc aacacctaca
     1261 ctggcacgat caaatccaac ctcggcgaga tccgggcgga tacgggtgac acgacaacgg
     1321 gcaacaaccc ctacgacacc atcttcgcgc tcgacctgcg caactccctc ctgcgcctca
     1381 actaccccaa caccgccggg accgctccga atgttgccga cagccgcttc tccacgaccg
     1441 cgccgatttc gatgcgatcg agttcgatca acatcgataa tgcgtcgttc agcggcacca
     1501 gtacgacctt cttcgactat gcggtcgccc agaccttcgg cacgacaacg atgcactccg
     1561 gggcgaacta catttatttc aggtccgccg acgcgggtag tgtgacgatg acctttaccg
     1621 atttgttgaa tccgaatccc ggcacaacgc tggagttcca gatcgacagt ctcaccggtg
     1681 gtgccgccca caatctcggg gtgaccgggt ccaagcaccg gatcctcaac accgcgttgg
     1741 agggcggggc aggagttccg ttcctgggcg gttgggcccg cacctcgggc ggccagagca
     1801 ccacttcctt aatggagttc gtcaaatatg tggtcggcag tggttacacc atgctcgcat
     1861 ccggcgatta tgcgacgaac acggcggagg gaacctgggc ttccgggcag aacgtcaaga
     1921 tcaccacgac cgcgaccacg accctcacag cggaccgcac ggtccagtcg ctcaatcttc
     1981 agagtacgac cgcccggacc ctgacaggca acccaggcac cacactcgaa atcgagtcag
     2041 gtgggctcat ctcccaaggt gcgacccatg tgatttccgt acctttcctg accgcgggag
     2101 ccggaggaga tcacgtgctc taccttcaca cgccgagttc gaacatcatc cgatctatca
     2161 tcaccgacaa cgggggaaat ccggtcagct tggtgaagga cgacggtggc accgtttcgt
     2221 ttctcggaaa caacacctac accggcacca ccttcctcaa cgaagggatg ttccgggaca
     2281 tcatcggtgc ccgcaatctc acggccctgg gatccggaaa cttcaacatg gcgggcggcg
     2341 cgatcggcca gtcggcctac gaaacagatc gcgacttcac ccgcgcgctc ggaaccagtg
     2401 tcggtgaggt gcaactgacc ggtggcggtg gaggcggcgg aggcagtgtc ggattctccg
     2461 cctacggagc tcccattgat ataaatttcg gaggttccgg tgccaccgtc gtctggggtt
     2521 ccccgacctt taatcctggg atcttcacgc tcaatggcgg gaatgccacc cacgtcgcca
     2581 ctttggtgaa cgacttcgat ctcggcggtg agcagcgcta catccgtctg gatgggggat
     2641 cctccgcagg caaccgcgcc gtcatgggaa cgatctccgg cgacatcacc aacggcggca
     2701 tcgtcaaacg gggtggcgga gtgctcttct ttgaagacgc caagtcctac accgacagca
     2761 ccatcatcca cgaaggcgaa ctctggctcc gcaatggaac cggaacggcc ggtgcgaacg
     2821 tgatcgggaa cgatatcatg atcggggcgg cctcaagact gaagatcgac gctccttcca
     2881 gcataggctc gcgtcagagc attattatgc agaaccagga tgacaacagc gcttccgcga
     2941 tcgcgttcgg acctggctac gggaacggag cagacatccg cttccacagc atcatcagcg
     3001 cgaacggcag cagcatcccg cagactggag cgtacgacat cacccttgcc aaccagcagc
     3061 ggggcaacga tcgtcgcaac cgggtggctg tgcaactcag tggcaaccac gttttcacca
     3121 cagatgtgat ggctcagatc aaagcggtgg ctccggatgt gcaggcatgg ttgggggctg
     3181 acacaggcaa tggcacttat gctggcagca ccattcctgc cactggccgc accaagaccg
     3241 gcagcattga ggctttccgc ctcggttccg gcggcggcac gctcaccatc gccaatgaaa
     3301 atgtccttga tggtgcgttc ccgctcatcg tcggagcgga agatcagacg gggcgcacca
     3361 acatcggcgg tgtcgttttt ctgccgaagg cgcaaaacta cagcgggatc atcagccaga
     3421 cggatggcac accacttgat gcgggaaatc tgatcggcgc tggggggata ctggtcgtcg
     3481 gtcaaaacgg cgcgctcaac agcggcaacg acaacatctc tcttcgtgcc ggcgagttgc
     3541 gattgggagt tgattcggca aactcattcc ttggtggcgt ggacagccag tacgccgcgc
     3601 gcaacgtcat caccaaatcc gcaaactcgg tgctgcggct ccagcccatc accggcgcgt
     3661 caaatggcgt gctcaagctc aaccagtttc tcatgcgcat ggacgcggcc gatcgcacat
     3721 tcagcctcaa ctccattggc acgcattaca cacagactgt tttcgatggt gatggcgtgc
     3781 tcgaaaatgg cgccacggcg cgcagcgcgt ttttcaacgt gggcaacgac aacagcttcc
     3841 aatccggtgt cggagtgctt gtgtttaatg gagcgctcag ccagactggc actggcgcgg
     3901 tcagcctgca aaaacgccag ggcggggtgc tcgttctagc aggcgacaac acctacaatg
     3961 gcacaaccaa tgtccagcag ggcaggcttg tgctcgcgca caccggcgcc gcgggcaatg
     4021 cgggcacgac gatcaacatg aacacgaaca acgaccggcg ctcggacctt gagttccggt
     4081 tggatggcgc ggggcccttt gtgttcgaca atgtcattaa caccacgggc ggcaatgacg
     4141 gctcgacccg tgtgatcacg gtgggttcac gaaccggcgc ctccacggac cagtcggtgc
     4201 aaatccccac gctgaccatc ggccacgcgg gtgctttcac ggtgaatggc acgggcagca
     4261 gcgcggtgtt tttggacggg ttcaacggtt accagttcga gatcacaggg acgctgaatc
     4321 tgaaccgtga catcgttctg cgcacccgcg gcgcgttcac caccatcagc ggtgtggtcg
     4381 ctggagcggc ggcaaacgac ctggagaaat ccgagcaagg cactctctgg ctcagtggag
     4441 acaacaccta tgcgggcaac accaccgtct ccaacggcta cctcgtcctc ggtcacgaca
     4501 acgcgctggg aaccgcgtcc accgatgtcg tttttcgcaa taccgctttc acccagatcc
     4561 tggcctccgg cgcgcgcacc atcagccgca atttcatcaa caccgccacc ggcagcaccc
     4621 agacccttgg cggcctggac gctggagcca agactttctc cggaaacatc aacctctcaa
     4681 cccgcggcat ctcccttacc gccgctcctg gaggcgacac cacatttacc ggcactgtca
     4741 gcggtgccca gggagtcacc aaaaccggcg acggcaacgt cgtgctggct cccgcctcgg
     4801 gcacgggcaa cacctacacg ggagtgacct ccgtgagcag cggcgtgcta gttggcttgg
     4861 ctcaggccgt gtcgggaagc ccgttcggcg agaacaatgc tttcaatgtt gccaatggca
     4921 ccctcgccct tcaaggagga ggcggtgcca ccaccacaag cacgaccggc gcgctcacag
     4981 tggacggcgg caatgccacg gttgcggtca atgctcccgc gggtgccacc acgttcacct
     5041 tcggttcact gtcgcgtgcg aataacgcca cgcttgtcct caaaggcggc tccacgaatc
     5101 tgggcggggc aaccaccgag atggtttcat tcaccgccgc tcccactctg acaaacggca
     5161 tcatcggacc ctgggcggtc atcggaggtc cgggcctcag cgacaatagc gggcactatg
     5221 ccgggtttac cggaggtaat gtggtgaccg ccacatactc gggcacggga gatctcgacg
     5281 gcttgacctc tgcctctgat gtgtgggatg caggcgccac cgccagcacg ctcaccggca
     5341 accggtcagc ccacgctttc cgcgcctctg ctgatgtggc tctcggcggc aacacgcttt
     5401 tcctcggctt cagcggccag gcgggcatca ttctcaacaa tggcgcggac atctcgggcg
     5461 gcacgctgag cctgggaacc aacgtgcttt ccatctacac cgatgacgcg gctgtctcca
     5521 ctctcggcgc gactgtgacg aacttccgcg gcaacaccaa caacactctg tcaaccggac
     5581 tgatcaagtt cggcccgggt aatttggaaa tctccgccca gcagtccttc caaggcaatg
     5641 tccaggttaa ccagggaact ttgagcttca gcgcggccaa tctggcgccg acttttgaaa
     5701 acctcaatgc cgtgatcggc tctgaggttg tcatccgccc gggggcgacg gtttccctca
     5761 acgggtttga ccaggagttc ggcaatctct cgggttcagt ggtggtcagc cctgtgctca
     5821 acacagccgg aacgctggaa ctgggcgcgg ccacgctcac tgtgggcaga aacaccgggg
     5881 accgcacctt ctccggccaa atcaccggcg gtgcgggttc gcgcctggtg aaagtcggcg
     5941 gcagccggct gatcctggac aactgggatc ccgcacggcc caattcgctg ggcacgcttg
     6001 aagtcctgca aggaccggtg cagtcccgcc tcaatgacaa ctcctgggct caagccatcg
     6061 gttttgccaa ctccattcct gccagcacgg aagtcctgct gcgcggcggc gagtttgagg
     6121 cctacgtcaa tggcgacagc accagcaatt tccagtttat cccgctgggt cacagcattt
     6181 ccgcgcgcga cggcaactcg gttcttgaca ccaaccggtg gcagggcggc ggcaccaaca
     6241 agatccttgg cttcaacaac ctgacggtgg acaaaaacat cctcactatc accggtggca
     6301 atgccgtcta tccgcgtttt gacggcacct tcaccatgac ggccaactcg cgtctgcaga
     6361 ttgacgcgcc gcttctgatc aacggttcga tcaccggcca ctacgccctg accaagacgg
     6421 gtggcagcaa ccttgaaatc ggtggcagca acaacggctg ggatggcggc acagttttgc
     6481 atgacggcct tctactcttc ggctcgcgcg tgcctgaagt ttcggagcgc tacatggccg
     6541 gcaccaacct cttcagctac agcgccaccg caaacctggg gacgcgggac attgtggtca
     6601 accgcagcac ggccgcccgc ttcaccgctc cttccaacgt tctcaaggac gacggccagc
     6661 gcgttctctt gtttggctcg gatggcgcgg gtctcccgcg cgtggacatc ggcgtggatg
     6721 cgccgttaac cgattattcc ctgcgctcca cgagcaaagg cgcgctgacc atcggcttga
     6781 acgacggctt ttacacccat gccattgacc agtccttgat tggagatggc aggtgggggc
     6841 ttggcgcctg ggcgacgact ttctacacag cgtcaacgct gggatatgga gtggacaatc
     6901 tctaccgctt cgcgggcgtg aacggcgttc tggggattac ccagtcacac gctctctccg
     6961 gcccggccgg actccaggtg ggcacggccc aagtgacgca gggctttgcc atcaacagcg
     7021 gcaacggcca ggcgcgcctt tatggtgacc agggctacac tggcaacacg ctcgtcttcc
     7081 gcaatcgcga ggctggcagc gtgcagaact tcctggaaat cctcggtgac agcgcctccc
     7141 cggttttcga tgtctatggc cgcctcatgg cgcgcggcgc gggccgcttc accaatgacg
     7201 cgggggtgca agtcaacacc gtcaatctgc atccgggctc cattctgcgt ctcgactacg
     7261 ccatggacgt caacgacacc atcatgatct cccgtctgga aaactccaat ctgggccttg
     7321 agtccactga aaacaaatgg ggtgactcca cgcccatgct gctcaacgga gccacgctca
     7381 acctggtcag ctccagtgca cgagtgaatc gtgaaacgat cggcgtgctc agcgtcaaag
     7441 gcggcgccgc tgtgctgctg gagcgcaatg gcaccaacgg ccagatcatt ctggagacgc
     7501 cctccatcac ccgcgtcggc caggccacct tcgatgtgcg cgaaaacgcc gatgaactgg
     7561 gccgcgtgga tttgcagagc cagaagttct tcatcaacaa cggcagcagc atggaggacg
     7621 cccaggggct gctgccggtg tggatgatca acccgacacg caacaccttc ctcacctata
     7681 atgaagatct tggggtgcaa aacgccgctt ttaccagcag caccacaacc gctggcaccg
     7741 gagccgcctt cctcggcggg ctcaccagcg cgtcagtggc cagctacggt gtgggcaccg
     7801 gtgatgccac tctggcgggc acagttgatg tgcatgcgct tcgcatcagc cacgaggcgg
     7861 ccagcaatga caccgtcctc actggcggcc agatcaacat tcacagcggc ggcctcatcg
     7921 tggacaaccg cgacaacgcg cgggtgaatt ttgacaccac gaatgtgttc ttcggtgacg
     7981 gcacgactcc ggtggaaggc atcatctact ccgaccagaa caacatcacc acccgcatgg
     8041 gcggcgtggt cacggcggca aacctcacca tgcacggcgc gggcaatctg cagctcacca
     8101 acactgccaa cgccatcagc ggcaacatcc agatgaacgg cggcaggctg ttccttgatg
     8161 gagtcggcac agcgggcacg ggcgccagca tcaccctgac cggcaattac ctgcaaaaca
     8221 acgacggcaa ccagatgccg gaactactgc tgcgcacact caatgccaac ggcgcctgga
     8281 ccaacggcat catcgtcgcg gagcacactc cctatgtgcg ggtcctggga cagtcgctgg
     8341 acggcacatc attaacgacc gttcgcaacc agaccatccc cagcctgatg atcgagggca
     8401 cgaacactca acagggcacc accttcatct tcggcacgac aagcaacacc aacaacgcca
     8461 acaactacaa cttgattgtc agtggcgcga ccacgctcgg cggcagcgcg cccatcggct
     8521 tccgcattga gcgcgggggc accaacatcc agggtggcgg cggcttcatg acgctggcgg
     8581 gcgctgtcac cggcacggct ccagtcatca aatcagggga cggctacctc gtgctccagg
     8641 ccgataacac aggcctcagc agtccggtga cgctcaaccg cggcatcatt cgcggccagg
     8701 gcaacaatgc caacaacttc ttcggcacgg gtgattacac actgaatttt ggcacggtgg
     8761 aaattctgca caacaacgcg ggcacctact tcaacgcctc cgggcaggac atctttgtca
     8821 atggcgcggc gagcatcaac tccaaccgca acggcggcag caacgcggcg aactacacca
     8881 tcggcgtcaa caatggtgac aacaccatcg tcacgtccaa tggcgcgtcg ctccgtctca
     8941 acgcggcttc tttcggggat gacttttacc tggaaagcag gctgatcatc aacgacagcg
     9001 ccgcctggtt ccaggacaac acggaggtat ggttccgcga ccagctcgtg ggcggcggac
     9061 gcctgacacg cacgggcatc tggcacacct atttcgacaa cacggtgccc aatgacaact
     9121 ggtcgggcac gctcgatctg caggcgggca ccgtgcgtgt gctgcgcagt gagaccaccc
     9181 ttggcggtgt gggcagctcc atcatcctca acccggcggc acaactcagc gtcaggacgg
     9241 tggcaaacct cggcaccgga aatggcatca cacaattgcg caccaccagc gctaccagcc
     9301 tgcctgtgct gggcatagcg ctgggcaccg aattcactgc cttgcgcgcg cattataacg
     9361 gactcactgc gactggcacc cgcaatggtg tgctggcgat ggatgccggg cagagcgtca
     9421 gtgtggatcc ggacatggcc aacttccaga acggcaactg gttcttcggg ggtgccacag
     9481 gcagcggtac tctggccgcg aactcggtgg ctccctgggg gcctggaggc aacaagttcc
     9541 tcattggcgg cggcagcggc accatcacgc tgagtccgac ggccggaagc gcccagtttg
     9601 ccggcagcaa ccagatgatc attggatccg cgctcaatgt ctttggctac agcacggtgg
     9661 tctttaacgc caacggcaac aacacttatg acggcggcac tctggtgact gtgagccgca
     9721 acatggacgg gggctaccga ggcatggcac tgagcattca gggtgggcgc gccacggaca
     9781 ccacctatcg cacaccgctg ggcagcggct tggtggatgt gtttggcgaa gttcgaattg
     9841 aacaggccaa cggcacggcg cgcaatgccg acaatgccaa tgccaacgcc tgggtgttcc
     9901 acccgggcag ccgcatccgc tttgacaatg acgctccctt tgccactgcc ggcaccgagg
     9961 gccgctgggc ggacaatgtt cccatcgccc tgaacaccac ggttcttgag atgtttggcg
    10021 acggcgcgac tcacccctac aacaccgaga cggtgggagc catctccgtt gcgggcgggt
    10081 ccgaggtggt tgtccgccgg cgcggggcgt ttctggctga aatcatcgcg ggtgacctga
    10141 cccgcgtggg gaatggcacg ctcatggtga caggcatgga taacaccacc aacaccattt
    10201 ccggcctggg ttccacggcg gcaaccaccg ccatgcgcct cctcgtcgcc aacggcgctt
    10261 cgctcatgaa caatggcatg gtggacccgt ggatcatcgg ccgcattgga ggccactttc
    10321 tgcgttatga tgccgcgggc ggcttccagc ccctgaccac ggccaatact cccaactaca
    10381 tcgtgagcac tgccaccacg attgacggcg cggtgcttcc tctcaatgac ggcacggaaa
    10441 tcctcgggct tgaggcgact ggagcggtta ctttgggggc gaatctggat gtgcatgccc
    10501 tgcgtgtttc gcgccagatc aatgccagca tcaacaaaag cgctgacggc tccttcagca
    10561 gcatcaccat ccgcagcggc ggcttgaact ttgctgacac cggcggcacc aaccgcagcc
    10621 cgacgatcaa cgccgacttg ttcttcggtc tcaacggcac gggggacgcc tatgtcttct
    10681 ctgacggcac caccggccag atcaacggca aaattcatgc ctcgaatttc atcaagagcg
    10741 gtccggcgga ggtttacatt cgtagcaacc aaccccagtt caccggcaac tggatcgtca
    10801 acggcggcgc ccttcgtttc accaccccgg gcgggcagag cagcggcgaa gtcatcctca
    10861 acggaagccg catgaatgac cgcgacaaca ctttcaacct cacggaggtc cgctacaact
    10921 tcaacagcgg cagcccggac cttttcacat ggactggcgg caagatcacc gggtacaacc
    10981 tcaaccgcat ctatgccatc accgccaatg accgtcttca gcagatcccc gccgttgact
    11041 tgcgcaccac caatacagtg gccggcacag ggcaggaagg cttgatgatc ttcctgctcg
    11101 acggtgcccg cagcacagtt cgcaccggca cggtcaccct ccacgaccac taccaagtcc
    11161 aggtggagtc caacacttat ggcacaggct ccacatccgg ctttcagttt gggtcgggca
    11221 ctggcgtcgg cggcctcaac aaccagggtc tcttcgacct gcgcaaggtg ggcgacggcg
    11281 tgctgactct cggcgacaac agcctcagtt tcaccggcag ccgcagcatc accatcggcg
    11341 agggcgcggt gcgcgtgctg cacggcggcg cctttggcgg cgctggcaac accggcaaca
    11401 tcgagcaggg cggcgccctg gaaatcgccg tggctggctg gtctccctcc gccacactcg
    11461 tgcagcagcc tggctccatg gaacgctggg ctgtggatgg agcgcgcatc ggggatgtga
    11521 ctttgtcttc gggcgtgcac tggcagatca tgcacaacca gaccggcacg cagaccgtca
    11581 ccctggacgg cggctccatc atggggtaca ccccgcgtga ctgggatcat gtcggcgtca
    11641 tccacagctt gggttccgga gtcaccatcc acctcgcctc caactccttc ctcggccagc
    11701 cttttgtatc ttcgaacaac tcgatttggg accaggcgcg catctacgac atcggcaaga
    11761 tcaaccagac cacagccagc aacccgaacg atcccggcct gcgcggcagc taccttcaaa
    11821 ttgacggcgt catcacgggt cccggcggct tgaccaaact gggccaggac atgatcgtgc
    11881 tcaacggtgc caacacttat gcgggagcga cctcagttga aaacggcatc ctgcagatag
    11941 gccgcaacaa cgcgctgcct gttggcaccg acctgagcac ggagaccagc agcggcatgt
    12001 tcgacctcaa cggctataac caggaggtgg cctcgctctc cggcgaccac ggcagcgtca
    12061 acaacggcgc gtttgacttg aacaccctca ccctcaacca gagcgccagc accacctacg
    12121 gcggccagat caacggcaac gtcagcgtgg tcaagagcaa caccggcagc ctgacgctga
    12181 ccagtgccag cggcatgagg ggtgacctcg ccatcagcgg cggcagcgtc atcctcggtg
    12241 cggcggggtc ggtcaacgag gcgcgcagca ttcagatcgg cgcgggcagc gtctttgacg
    12301 tcagcgccgt gaccggcggc gtgtattcct acgatggcag gatcagcggc ggaggcgtcg
    12361 gcgccctgcg cgcggacaac gccagccgcg cccagatcat cggcagcctc atcgtgaccg
    12421 acaacattgg cgccatcgcc cgccagggat ccatcagccc cggcaactcg gccgggcacc
    12481 tgtatgtgag cggtgatctc acactgggcg gcggtctctg gggcacgagc accaagaccg
    12541 agcgcctgac cctcgagctg ggcgcgccga cctccaccct ggcggcgctg ggctgggacg
    12601 gcagcaacgt ggccgactgg ctggtcaacg tctcgcctga cgtgctcaac gggctggcgg
    12661 gcaacctcag cgggcacgat tatgtgaacg tgggcggtga gctgacgctc aacgagcacg
    12721 gcggcatcgg cgtgacgctc atcaacggct accagccgca gtatggggac gtgttcaacc
    12781 tgctggactg gacgagcgtg agcgtgggcg gctttgacgc ggggccgaca ccgcgcagcg
    12841 gcggggagct gggttacgac ctgaaccttc ccgacttgac agccttccaa ctgacctggc
    12901 acacggacct gttcgccgac tacggcgtga tctttgtggt gccggagccg ggccgcatga
    12961 tgctcctgtt cttcggcctc acgggcctgt tcctcaggcg caggcgttga tcgtttattt
    13021 ctggatgcac accacggcgg attgcttctg ccgtggtgtt ttcatttttg cgcttttctc
    13081 atccgccgct gaggcgcgga ctttgcgggg cgcttgtcat tttgtctcaa cgagtctttc
    13141 tcagaggggc ggaaacggca gcggaccact gtttcccagc ccggggcgct gtctatgctg
    13201 agattggcgc cgatgatgtt cgcgcggtgg cgcatgactc taaggccaat gccatccgat
    13261 tttggatggg aaggatcgaa tccgatgccg cggtcgcgca cctcaagaat gcatccgcct
    13321 tcgtgccggc ggagggtgat tttgacaaga tccgtcctgg agtgcctgac ggcgttgctc
    13381 accgcctcct gggcgatgcg gtagatgtgt gtctcctgcg cggggtcgct cacacgcaca
    13441 gaggaatcta tgtcagtggc gcagtggatt tgtccggcgg cattgacatg ctcggccagc
    13501 aatttcaaac ctcccgccag gccgcggttt ttgaccgcgg ctggtgagag tccgtgggag
    13561 atgcgccgcg cctcatgaac cgtctccctc aggatttcat acacgcgcgc cccatctttt
    13621 ttttgctggc cttcgagtcc ggcgacaagc atctccagca gggtggccgc tccggtgagc
    13681 atctggccaa ggccgtcgtg gaggtcgttg ccgatgcggg tctgctcgtc ctcgacgatg
    13741 cgcaccagtt cggactccag cctcatgcgg tcggtgacat ccacagcggt ggtgacaaag
    13801 ccgcccggag agccgtccgg gcggcgcagc agggaggtct gagtctcggc atgacagacg
    13861 ctgccgtcct tgcggatgaa gcggaagggg tccgcaacac gctccacctc gccgctgatg
    13921 agctgctcca tgcgctctcg caccaggtca gcctgggagg gatccagatg gaatagccgc
    13981 catagttcct gcccgcttac ttcagccgcg gagaagccgg agatgcgttc tgcggcgcga
    14041 ttcatgccgg tgaccacgcc attctcatca agaatcacga tcatgacgct ggcgtcgcgg
    14101 atgagagcct cgtaaaactg gttcttttcc cacagatcct gaagcgcctg cttctgcgct
    14161 gtcacatccg tcattaccat gacaccgccg tccagacctc ccgcctcatc catgaaaaaa
    14221 gtcggtgaga cctgggtgca gaggatctct ccccgttttg tgacgagttc catctcaaga
    14281 gtggtcctcc ttcgggtgtt ccgtgccgct tcctggagct gaccggagac caagccgcgc
    14341 gtggggacgg agcaaagctc gattattccg gctccaacca gggcgtcttt ttttctgccg
    14401 accagtttga cgaaagtgcg gttgactcct gtgatgacca tgccgcggtc cacaaagacc
    14461 accccggctg tgttgttctc aaaaaggccc cggtaatgcg cggcggctct ccttgcttcc
    14521 agttcgcgct tctccccggc cgcatgcgcc gcattgccgg aggtcttttt gacttcggat
    14581 tgattgtgtt ctgtccgcct catgcttcgt catcctctgc ccactcctcc atgagacggg
    14641 ccagcttgcg tttcagcccc accttggcgg cggcggacat gcggtctatg aaaagcacgc
    14701 cgttgaggtg gtcggtctca tgctggaaag cgcgcgcgag caggccgtcg gtctcccagg
    14761 tctgtgttgc tccgtccaag gtctggaaag tgactttgag tgacgcgctg cggcggatgt
    14821 cgccgcgcag gcggggaaag ctcaggcagc cttcctcgtc ggtctccttg tctttgccgg
    14881 gttcgagcac aggattgaga aaaatgaccg gcatgcgcgc ggacatgtcc gccggctcgc
    14941 cattgacgcg cagaaagctg acgcatttgg gattgtgtga aacatctatg accgcgagtt
    15001 gcacatcctg gccgacttgc ggagcggcca agccaacgcc gttggcggcg cgcatcgtct
    15061 cgatcatctc cgccgctagt cggcgcacct ccggcgtgac ttctgtgacg gggcggcatt
    15121 tcgcgcgcag gacggggtct ggatagcgga cgattttcag gggcatgaca atacaggacg
    15181 cccagtggta gcagcatcgc gcgcgcggtt cacgctcttt cttgaaggca tctcggcgcg
    15241 agagctttca ggtgtttcgc gcgttatttg ctgccgccgc catgcaccgc ctcattggtc
    15301 tattcctcgc cgccagcaca attcacgccg ccgactggcc ggtgtggcgt tacgacgctg
    15361 gacgcaccgc cttctcaccg caggccatgc cggaaaatcc cgcgctgctt tggtcccgcg
    15421 agctgcctcc gctgcgcccg gcttacaaag acccgcgcct tcagttcgat ggcggctacg
    15481 agccggtgtg cgcgggaggc aggctcatcc tgggcagttc gcgcgaggat tgcgtcatgg
    15541 catatgacgc cgagaccggg gccgaattgt ggcgcgtcct ggccggcggc ccggtgcgct
    15601 gcgcacccgt catcgttgac gatcttgtca tcttcggcgc ggacgatggc gtggtgcgct
    15661 gcgtgcgagt ggaggatggc gcgcaggtct ggacaaaacg cgccgtgcct tcaggccgca
    15721 tgctgctggg taatcaacgc cttatttccg cctggccggt gcgcggcggg ccagtggcag
    15781 aggacgggcg cgtctatttt gccgcgggcg tctggccgct ggagggggtc tttgtctttt
    15841 gctgggatgc ggccactggg gatgaaattt ggtgcaacga ccgctgctcc tacctgtatg
    15901 gcatccaccc gcacgacacg caggccatgg gcggcctggc gccgcaaggc tacttgctgg
    15961 tggatggcgg ggatctcgtg gtgccgtgca gcaacgccta cccggcgcgg ctcgacctga
    16021 aaaccggcgc gctcaagcag ttcgagctgc cctccgccgg gcggctcacc ggcggctggt
    16081 tcgcctccac accggcggaa aagaaggcct tccgtctgca acgctggggc ctgctcattg
    16141 acagcggggt gaatgccaag cgccatgagg acaagccgcg cgccgagggc atcgcgggca
    16201 tccgccgcag tcttcacgcc gcaggcaggg aggcgggatt cgactcgctg aagctgcctg
    16261 gactcgcagg cagggtccac agcgccatcg tggccgatga aaaatgcttc gtcgtcaccg
    16321 aggatggcat cctgcacgcc tatggcacgc tccaaggcga ggcgaggcac tggaagcgcg
    16381 agatcagcgt ccagactgcc ggagaggcgc ttgcaaaaaa atgcctcgcc gccgccggca
    16441 cggagcgtgg ctatgcactt atgatcggcc cgaatgaacc cggcttgatc gagtccttga
    16501 ttctcaacag ccacctccac ttgatcatcc ttgccacaga tgcggcggcg cattaccggc
    16561 tcaatgaagc cggactttac ggcgagcgcg cctcagtcgt ggacatccac gctgtcgcgc
    16621 aacccggagc gctgccgcct tacttcgccg atgtcatctt tgccctggag ggcggtcaag
    16681 aagacttcct gcacacactg cggcccggca gcggcagggt catcggtcct ggtgcgagtg
    16741 tcatccatgc gcgcggtccg ctggaagggg ccagcgacta ttttgccgac tggcagccaa
    16801 gccaggacgc gctcgtgcgg gcgccgctcg gcgtcttgtg gtttgacgac gcgctcagcc
    16861 acttcaaacg ctcgccgcag ccgaagatca tcggcggcgt catgatcacc agcgacaagg
    16921 actggctcga cgcctcgaac cgcaaaggca aagtggacta ccgcctccag gcccccgtgt
    16981 tcagcgacat ctacaccggc cgcgttctcg atgaatacga ggcgccggag ctgcgcgcca
    17041 aacacggcac cgtggacatg gaaagcatcc agccagccca ataccgcccg cccacccaga
    17101 aggacgactg gaagcccggc gcgccccagc ccggcatgag gatcagcccg ctcaccggac
    17161 tggaggagcc gcgcgtcttc ccgaagagct acggctgcga cggcggcttc gattacggcg
    17221 gcatctacac cttccgcagc ggcacggccg ccttttacga caagaaaatc gaaagcggca
    17281 ccatccacat cagcggcccg cgcagcggct gcacgaacag cgtcgtgccc gccggcggca
    17341 tcctgaacgt gccctattat tacgagggct gcacctgctc ctacccgctg cccgtggcgc
    17401 tcgccctcac cagcatggac gagcgctttg agcaatgggc cacctgggga tcgcagtcca
    17461 aggacgaact gcgcggaaag atcgagcgca tcggcatcaa tctcggcgcg ccgggagatc
    17521 gcaatacccg tgacggcacc ctttggctcg accacccgaa tgtcggcggc ccctctcctg
    17581 aaattgaagt cgagaacgag cccgccctac ccgagatctt ctaccgccat tccatctggg
    17641 tggaaggcgg cgagggctgg ccctgggtgg ccgccagcgg gatgaaaaat ctacgccggc
    17701 tcaccctgcg cggactcaag ccgggctctt atctcgtgcg cctcactttt gcctgtcctg
    17761 acggggaggc gcgcgtgctg cgtgtttccg tgcagggccg catcgtctcc gagcgcctgg
    17821 aactgccgag gccaatgacc gcgcgcaccc agacccatcc cgccatcccc atccgcgacg
    17881 gctccctcac actggaactc gaagccctcg aaggcggcac cctgctctca ggcatcgagg
    17941 tcatccggga gggattgggc attggaaatg tgcccgaaaa cgcgcgagcg cccggacgcc
    18001 tgtgatgaac tcaccgctcc gtatttttac cgccgcagcc tgcctgctgc tacccgccac
    18061 cctgacgcgg gcgcaccagg tgccgagcat caccgtcgag gccatcttcg ccgccgacca
    18121 cacctacacc ctccgcgtga acctggaccc gcgagtcttt ctcagcgccc agcccgcctc
    18181 gctgccgccc gtggaggccg cgtggtacct ggagcagtcg gaggaggaga aaaaacagac
    18241 ccaccttatg gcgtgggact acttgaaaaa aaactttcaa atctcgttta gcggccggtt
    18301 ggcggacttt ccggagtacg ttttcacagc cattgatgga gccacgcttg atcctttgca
    18361 gccggccacg ccggagactc acctgctcgc agccgccaaa tctcgcgtgc ctgacgaggc
    18421 aggagatttc gtgctggcat ttggtccgga agccaacgtc agcctcattt tgctcatcgg
    18481 tgaggagggc agggcagggc accgccctca gggcttgttt cccggcgaaa caagccggcc
    18541 gcataaaata ccgcaagcaa cggctgctgt tgagggagac gcaggagaaa ctgtatcgtc
    18601 cactgaaagc agtggaagtc aggcaattgc aatggctggt ttgatcctcc tcgtgctttt
    18661 gcttttcacg ccggccatcc gtcggaggat cgccagtgga gcacagacca aatgaaagct
    18721 caatgcacgg cattcggtcc gccgggtgcg gctggcgatg gatttttggc gcacccctca
    18781 ggattcgaac ctgaaacctt ctgatccgta gtcagatgct ctaatccgtt gagctagggg
    18841 tgcaattccg gtgtttcgga ttgcgcaaat acttgcgaga gcgtgaggct atcggatagc
    18901 tcgttcttgt caaacggtga gaaggaaaaa tttgaggttt cgaactgtgc gctggtgcgt
    18961 gttgatgacg cggacaagcc atgagggagc gcgtcaaatt ttcggcgacg ttttgttgac
    19021 agattgaact ttcagtattt aatgaaaaca tgagcgccgc acttccgaac tgggaaaaag
    19081 cccgcgacga gtttgtcgcg caatgggggg cgctggggag tcattggggg atcaaccgca
    19141 cgatggcgca aattcacgcc ctgctcatga ccgcgcccga gccgatgcac accgacgaga
    19201 tcatggaacg gctgcgtgtg agccggggca atgccaacac caacctgcgc gagctggtcg
    19261 gctggggcct gatccgcgtg gtgctcaaga aaggtgagcg gcgcgagtat ttcgaggccg
    19321 agaaagatgt atggcagatg ttcatcacca tagccaagga gcgcaagcgc cgggagcttg
    19381 atcccgcgct catggtgctg aggagctgcg ccgagcagac ccaaagcgag aaaaccggcg
    19441 cgggtctgga ctttcaccgg caaatgcgag agctgcagga gttcgtcgaa ttcggcatga
    19501 aggtgtccga cacagtggct tccatgaagc acgcctccgc tctccagtgg gccatgcggc
    19561 tgcttggatg atctttttat ttggccgtaa ctttcatata ttatcgaaac tacagaataa
    19621 tacgccatga atgctcccgt tttcatttca cctccgcccg gcgcgcatgc tgtcgggcgg
    19681 atgcatcccg attcgcgaat gaccaatcct ttgcgtggaa aaattgtctg gagtccggtc
    19741 aagtccctct ggttcaccgg ccatgctctc gtggcactgg ctgggggctg gctgacggcg
    19801 ggtgtctggc ctgtggcggt gtgctgcgcg ctgaccgtgc tcactctctg tctcgggcac
    19861 acgctcggac tgcaccgtct gctcattcat cgcagttttt cctgcccgat ttgggtggag
    19921 cgggtgcttg tgcatctggg cgtggttgtg ggcatgggcg ggccgtttcg catgctgctt
    19981 ctgcatgaca tccgcgactg ggcccagcgg cagccgcgca gccatccgtt ttacaccaac
    20041 caggcgcatt ggtggctgga ctggttctgg cagatgcact gcgaattgcg cctggagaat
    20101 ccgcccgttt tcaagatcga accggctgtg cgaaacgatc catttctgcg cttcatggag
    20161 cggcactgga tgctccagca ggtcccttgg gcggtgctgt gccatgcgtt ggcgggagtg
    20221 ccgggactcg tgtggggtgt ttcggtgcgc atcgttgtct ccctcaccgg ccactggttg
    20281 atcggctggc tggcgcacaa tgttgggcgg cgggaatggc acatggaggg acactcggtt
    20341 cagggtttca acctcccacg cctcggtttg ctgacgatgg gcgagtgctg gcataacaac
    20401 caccacgcct ttccaggctc cgcgcggctt ggcctcgctg ccgggcagca cgaccctggc
    20461 tggtgggcgc tgcggatgct catggccgcc gggctggcat gggaggcaaa gacacccgcc
    20521 accctgcccg accgccccga gcgcaagcgc ctgccagccg cgccgaaatc acgcgccacc
    20581 accttcttca ccctctaaac caatcccaaa tccacataac gccatgaacc acgccatcat
    20641 catccactac accctctacc tcgcgctcgc actctgcatg accgtctggg tggcacgcac
    20701 cctgcacaaa aacggccgcg tcttcctcgt cgaatgcttt cacggcgaca cccacctggc
    20761 cgacagtgtg aatcaccttc tcgttgtcgg attctacctc gtgaacatcg gcttcatctg
    20821 cctctttctg cgcgccgccg gcgaagtgga tggcgcgcgc ggtgtttttg agatgctcag
    20881 cgggaaaatg ggcgtcgtca tgctcgtgct gggagccatg cacttcatga atctccttct
    20941 gcttgcccgc atgcgccgtc gcgcgcgtct cgccgccatg ccgccgcccg tgccccccgt
    21001 attgcgcgtc agcgcctgaa ctcgcgcctt cccccacagt catgcacaca ctcaccatct
    21061 tctatgacgc gcgctgcgga ctttgctcgc aggtgcggca ctggcttctg gcgcagcccg
    21121 cccacgtccg gctggagttc ctgccttacg actcccccga agccgcgcgc cgcctgcccg
    21181 gattgcgtca cctgcgcgcg gacagggaga tcgtcgtgct cgccgactcg ggcgaggtct
    21241 ggcagggtgc cggcgcctgg atcatgtgcc tctgggcgct gcgtgaatac cgctcgtggt
    21301 ccgccaggct cgccgcgccc gccatgcagg ccgccgcacg ccgcgtggtg gagtggatat
    21361 cccgccaccg tgttggtctc tcgcgacttc tgcgcatgaa aagcgacgcc gatctgctct
    21421 ccgcagccgc ccaggcaccc gaggctcccg cctgcgccgt ccggcacgac cttgatctca
    21481 ttgactgaac tcgacaggct tgaaggcttg cttcctggct gaggcgaggg atttcatgac
    21541 caactccatg ccggcattgc caccgctcat gacacagccc aggcgtttgg catgacgcac
    21601 gggggacgct tttttcaagg ccgcccagga cagcgcgccg gcgccttcga cgaggttgtg
    21661 cgtgttttcc aaaagctccc gcatggcaaa aaggcattcg tcatcattca cggtcacgat
    21721 ttcatccaca tgacgccgga tcagcgccag tgccccggcg ttcggcgtgc tgcatgccac
    21781 gccctccgcc acccgtgtgg cgctggtttg cgtgcgcaga ctgccgctgg caaaggacag
    21841 cgcatacgcc ggcgcatgcg cgctgaccac gccgatgagg cgcgtcttca atcccagcgc
    21901 cgccttggcc gccgccatcc cgcagatgcc ggagccgagg ccgatgggca catagaccgc
    21961 ctccatgtcc ggcaccgagc gcagaaactc cagcgcgtag gtggccacgc cgcgcaccag
    22021 ccaggggtga aaggacggca ccgcgtgcag gccctgcgcc tccgccagtg tgcgggaatg
    22081 ctccagcgcc tcctgaaact cgcgcccgtg ctccacgagc tcggccccga gcgtgcgcat
    22141 ggcgcggttt ttgtcgggat tgttcccatg cggcaccacg atcacggcgc gcagtcccaa
    22201 gcgccgcgcc gaccacgcga tggactggcc gtggttgccc gtggaggcgg cgatgacgcc
    22261 acgcacgccc ggctcccggc gcagcaattc atccagatac acaatgccgc cgcgcacttt
    22321 gaaggcgccg accggcgtgt ggttctcgtg cttcacccac acttccgcgc ccgccatctc
    22381 actgagcaaa ggccaggtgt gggccggtgt cggcggcaca tgcggcgcga tgagcgcggc
    22441 ggcggactcg atctcctcca gagtgggcaa ggcgggctcc atggagcttg ggcggcgtca
    22501 gcccttggtc acgagttcgt cgaggttcag caccaggttc gcgatcatcg tccaggcggc
    22561 gagttcggcg gggtcgaggc tatcgtccgg cttcgactcg ccctgggaga tgagctgctt
    22621 cgcatcctcc acacgggcgc ggtaggcggc gcgatgctgc tccagcgtct ggcggatcag
    22681 ctcggcctcc tcggcagtcg ggtggcggca ggtggcaaag cggaacgcat gagtgatgcg
    22741 gctgtcatcc gtggagccgg gaatttttaa aagctgctgc gcaaaatggc gcgccgcctc
    22801 cacatactgc acgtcgttca tgaggttgag tgcctgaagc ggcgtgttgc tgcgctcgcg
    22861 gatgaggcag gcggactcgc ggttcggcgc gtcgaagttc gtcatcgcag gcggcggcgc
    22921 ggtgcgcttg tagaaggtgt aaaggctgcg ccggtagagg ctctcgccct tgtcctgcac
    22981 atagaagcgc gtgttgctgc cgccaaaggc caccggctcc cagatgtttt ccggctggta
    23041 ggggcgcacg cccttgccgc ccaccttcag gctgagcagg ccgctgatat aaagaatgct
    23101 gtcgcggatc acctccgcat ccgcgcggaa acgcggaccg cgcgccagca ggcggttctg
    23161 cggatctttg gccgcgctct ccggcgtgaa gcgcgtgctc tggcggtagg tgtgcgagtt
    23221 cacgatgagg cgcacgagcg ccttcatgtc ccagccgctc tcgcggaaag tcaccgccag
    23281 ccagtcgagc agctccgggt ggctcggcgg ctcgccctgg gagccgaagt cattgctcgt
    23341 cttcacgatg ccggtgccga agaactgctg ccagaagcgg ttcaccgtca cgcgcgccgt
    23401 cagcggatgc tcaggcgcga gcagccagtc cgcgaggtcg agccgcgtgg gctgcggcgc
    23461 ttttgtcttc atcggtggaa atgccgccgg tgtcgtgcgc gccacctttt cccccggctt
    23521 gtcatagagt ccgcgctcca tcacaaagct ctccctcggc tcgggcaggt cggccatgac
    23581 aaaggtggcc gggatcgtct cttcgagcgc cttctttttc gcctccagcg ccagcttctc
    23641 cgcgcgcggg ccgtccacga tctcacgcgc gccctggtat tcgttttcga accaccacgc
    23701 cttcagcttc gccgtctcct ccgcgctcca ctcctcaggt ttcttgccgc gcacgagatt
    23761 gcgcaactcc gtctccagga aggacagcgc cttgccctgg ttgttcttcg tccacacctt
    23821 ccacgaccac tgcgggtcct tcgccggctc cacgcgcgag ttcatcgcca gccggtccca
    23881 cgtcaccgtg ccgccgtatt gggtgaaggc gaagccgttc accttcatgc ccggtttcag
    23941 cgccaggcgc tccgcgttca cttgcagctt catccatttg cccgcctccg gcagcgcgcc
    24001 caccttcaca ttctccggcg tgcggacttt gccgaaagga atcgcaccct cctcgcccca
    24061 cgtcgcccgg tgattccacc ccgccgtgtg gaattgcagc atcacggccg agggggggtt
    24121 cgccgggtca atgaagcact gcacgctgat gacgccgttc gccggcacct cgaaggacgc
    24181 gccgcccgag aaaaaatcct gcgccacctg ctccgccgtg cgggtcagcg cgcgcttgcc
    24241 gctgaagacc tggccgtcct ccgccgtgac gaacttcgtc ggcgcgcccg cgtgctccac
    24301 cttcgccttg gcggggaaat catcatcgaa ccacaccacc tcgctcgtct gcgcgggcgg
    24361 cggcggcgtc agctcggcgg ggtccgtgta ttggatggcg gcgatggcct cgcgaatcgc
    24421 ttgttgtttg gcggcaatgt ccgcgtccag cttggcgagc tggctcttct gctcgtccgt
    24481 gctcaggcgc aggatgggcg gcgtgagcaa aatgttgcca tccatcgccg ggtccgccgc
    24541 gctgttgaag aaggagtaga gcgaataaaa ctccttctgg ctgatggggt cgaacttgtg
    24601 gtcatggcac accgcgcagc ccgccgtcag gcccatccac accgccaccg tcgtctccgt
    24661 gcggtccacc gcgtagcgga agacgaactc ctcattgatc gagccgccct cgctcgtcgt
    24721 cacgttgcag cggttgaagc ccgaggccac ctgctgctcc atcgtggcat tgggcagcag
    24781 gtcgcccgcg atctgccagc gcgtgaagtc ggcaaaggac aggttgtcgt tgaaagcgcg
    24841 caccacccag tcgcggtagg gccacatgct gcgctcattg tccaggtgca ggccgtgcgt
    24901 gtccgcgtag cgcgccgcgt ccagccagta gcgcgccatg tgctcgccat agcgcgcgga
    24961 cttcagcaga cgctccacca cgcgctcata ggcgtccgcg ctcgtgtcgg tgagaaaggc
    25021 gtccacctcc gccggcgtcg gcggcaggcc ggtgaggtcc agcgtcaccc ggcggatcag
    25081 cgcctctttc gaggcctccg gcgcgggctc cagaccctcc tgcgccagcc tcgcctgaat
    25141 gaaggcatcc acagaatttc gaatttcgaa tttcgaattt cgaacttccg gcaccgccgg
    25201 cttccgcggc gtgatgaagc tccagtgctg ctggtattcc gccccctgct cgatccactt
    25261 cttcaaagtc ccgatctgcg ccggcgtcag cttcttgtga gactccggcg gcggcatgat
    25321 gtcgtccggg tccgtcgtca cgatgcgctg catcaccgag ctttgcgccg ggtcccccgg
    25381 cttgatgacc gccacgccgt cgagcaccgc atacgcgccc tccgccgtgt ccagccgcag
    25441 gtccgccttg cgcttctttt catcgaagcc gtggcaggcg aagcagttgt cgctcaggat
    25501 cgggcggatg tcccggttga agtccagctt ccccgccgcg tgggcggagg cggcgagcaa
    25561 aagcagaagg gtggtggtgg cgggggcgga gcgcatcccc cctgatacga agcgccccgc
    25621 gcgcggattt caccgcttca aaaagggcgg caaccaaggc agcagcggtt tgcggatttc
    25681 ctccctcacc agaccgggca ccgcgccgaa gccgtgcagg gccgccgcgc cccccggctt
    25741 gggcaggcgg aagtcaaaga cactcacccc gcgccgcttc tgcccctcgt agctgcgccc
    25801 gtcgctggcg ccgaagatga ccggcttgcc gtcgcccttc cggtggccga gaaaaagcat
    25861 gacatgcgtc acgggcagcg cccgcttggt ggactcgtaa gtgcccgacc agaacagcag
    25921 atcccccggc gccagcttcg caaagaccgc gtcctccagc gactccgcct tctccgcgcg
    25981 ctgatacgcc cccttgtcca tcacccactg gcacatctgg tccgactggc gcggcgcctc
    26041 cgtcacaccc cggctgtgca gcagatggta aatcgtcccc gagcagtcca tgcccccgcg
    26101 ccccggatcg tgcgaggcga aggtgtacgt caggttcatc cgggtcagcg ccagcgccgc
    26161 gcggatcagc tcccgcaccg gctccgggtt cttgtcaaag tcccgcagct ccgtcacctc
    26221 cagcgtcgag accctcgcgc ccttgtccgg ctccgccccc gccgcgcagg ccgccagaac
    26281 gaaaaagcca atgatgggaa tgcgagggct ggagatcatg cgaaagacac ggggcggctg
    26341 aaaacaagcg ccccgcccga cccatagcac aaccgcgcgc gttgtggagg cccgttcatc
    26401 cccgttcacc ccggccccgc cgccgcgccc cctcttgcaa accccgcgct ctggcgggcg
    26461 cgcctgcggc accgtgtagg cgcattcgcc ggagactgtg tcaaaacaca cttgaagatg
    26521 tgataaca
//
LOCUS       DAOMYJ010000049        26488 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Prosthecobacter sp. isolate AMR_MDS_5629
            NODE_670_length_26488_cov_21.909469, whole genome shotgun sequence.
ACCESSION   DAOMYJ010000049 DAOMYJ010000000
VERSION     DAOMYJ010000049.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867850
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prosthecobacter sp. (wastewater metagenome)
  ORGANISM  Prosthecobacter sp.
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Verrucomicrobiaceae; Prosthecobacter.
REFERENCE   1  (bases 1 to 26488)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 26488)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.30
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:34:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,742
            CDSs (total)                      :: 4,684
            Genes (coding)                    :: 4,676
            CDSs (with protein)               :: 4,676
            Genes (RNA)                       :: 58
            tRNAs                             :: 55
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26488
                     /organism="Prosthecobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_670_length_26488_cov_21.909469"
                     /isolate="AMR_MDS_5629"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1965333"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            145..1350
                     /gene="amt"
                     /locus_tag="PLP58_07235"
     CDS             145..1350
                     /gene="amt"
                     /locus_tag="PLP58_07235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018969841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008519 - ammonium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0072488 - ammonium transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HRJ08344.1"
                     /translation="MMIAAALVFIMHLGFATVESGLTQSKNTVNILFKNTLIPCIGLL
                     TYALCGFNLMYPGFADEDPQFWFKFAGFGITTDEAGVTSAYNVGYTYWTDFLFQAMFA
                     ATAATIVSGAVAERVKLGTFLVFSTIFVAVCYPIAGSWKWGNGWLNRLDTPFYDFAGS
                     TLVHSVGGWGALAGVMLLGPRMGKYTSDGKIKPILGHSMPLATIGVFLLWLGWFGFNG
                     GSVLSADPGLVSLTLVTTSLAAAAGGVSAALASVIFLKKPDLSMALNGILAGLVGITA
                     GADQMAADTSIYIGAIAGVLVVLSVLFFDKIKIDDPVGAISVHLVCGIWGTLAVGIFG
                     AKAGTAQLMSQLTGVAAYAVFTFVFAFVLFLILKIVMGLRVSPEEESEGLDIGEHGNV
                     AYPNFIANH"
     gene            1508..2950
                     /locus_tag="PLP58_07240"
     CDS             1508..2950
                     /locus_tag="PLP58_07240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009962184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008519 - ammonium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0072488 - ammonium transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HRJ08345.1"
                     /translation="MIRTLQLCRTLACGVLAATLLLSGQIQAQETQPATAATPTLEEL
                     AADLADVQAYINNGARVTEAGKSKIAGPGPGHNAWQMVSTALVLFMTLPGLALFYGGL
                     VRKKNVLSVIAQCMGIAGLVTILWWACGYSLSFGTGNAYVGDFSYKFLNGVEPGNVGA
                     GYHWISDSMWCMFQLTFAIITPALIVGAIAERMKFISVLVFTALWMFAVYFPFAHMVW
                     AGSGFMCGPLNADAGIKAIDFAGGTVVHMTSGWSALVLCIMLGRRRGHGKEPMAPHSM
                     VLCAIGTGMLWVGWYGFNAGSALGADAIAANAFTTTTLAAATAGFVWALAEWVLRGKP
                     SVLGFCSGIVAGLVVITPGAGFVTATSSVIIGVLAGIVPFLAVAYLKNALGYDDALDT
                     FGIHGVGGTLGAILTGVFADEKANSVVAGLKDGLVMEQFKAVGLTIVWSVAATVVITL
                     VVKMLVGLRADAEVESLGLDLAEHGEAGYE"
     gene            3104..5029
                     /locus_tag="PLP58_07245"
     CDS             3104..5029
                     /locus_tag="PLP58_07245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018968565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon starvation protein A"
                     /protein_id="HRJ08346.1"
                     /translation="MSTLLIASGAFVLYIVAYHTYGRWLSQKIFKLDPRATVPSVELN
                     DDRDYVPTSKGIVFGHHFTSIAGTGPIVGPALAIMWGWVPALLWVLFGSIFIGAMHDL
                     GTLVVSLRNRGMTVGEVAGRLLNKRVRLLFLVLLLFALWVVLAIFGWVIASVFVQFPE
                     SILPVFLQIPIAIWIGVKVHRQGKSLLWPSIIAVSLMYATVWLGAGCPGMEWTGGTLG
                     AAIQSLNQSLAAWPLWMWVAVLLAYCYAASVMPVWILLQPRDYINSLQLVSSLVLIVV
                     GLVVAAFIGTGGENAAPLVMAAPAFNASPLNAPPIFPFLFVTIACGAISGFHCLVSSG
                     MSSKQLKCETDAQFVGYGSMLLEGFLATLVILAVGAGIGMGWQQYPGLQGAELWGTVY
                     KDWKLVTGGSAIAAFVVGSANFVQTLGIEPVMARALMGVLVASFAGTTLDTATRLQRY
                     VVQELAATFAPRVSPTAMAAEAYDTEFERGVVRKGFSLNPLVWLTNTHGATLFAVGTA
                     FLLALFPAPGAEWSWDTIGKGGLILWPLFGATNQLLAGLAMMVISFWLLRRGMPTWFA
                     ATPMAFMLAMPAWALYVDVQRWLAGGSWVLVGVGLIMLAIEVWMIIEACLLWPKVRGV
                     LEAPLPPLPARTGAQTA"
     gene            5037..5666
                     /locus_tag="PLP58_07250"
     CDS             5037..5666
                     /locus_tag="PLP58_07250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ08347.1"
                     /translation="MRCPSCRHPAAEHLAVCGHCGFCLEAVDRALGAPPAMTAPLTDA
                     AAILTRREKTAVLGALNAFSSRHPQLVPVCVLANVPPGVPVEIYTFWIHNRAGFFSLS
                     ESGGRNHGLLLVLDPQANSAAAMPGYGLEPFIGEDDLAPALAAARLSLDSGDPARAAG
                     VFFREMSARLLIISENLPRAFGYDETRPWLDSEGGDDWTIPSHEAADVY"
     gene            5847..7898
                     /locus_tag="PLP58_07255"
     CDS             5847..7898
                     /locus_tag="PLP58_07255"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009962423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009678 - pyrophosphate hydrolysis-driven
                     proton transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-translocating pyrophosphatase"
                     /protein_id="HRJ08348.1"
                     /translation="MNSLLLDQGVLVSIALAVAGLVIAVLLIRQILASSAGNEKMALI
                     GGAIQEGAKAYLNRQIRAVGLIAIIIFFVVWYLRDGMTSAGFVVGAVCSMVAGYIGMM
                     IAVRANVRTAQAASVSSHAALKVAFNGGAVTGLLVVALGLLSVGMFYLVIEKMAGVRA
                     AIDSLVGLALGSSLISVFARLGGGIYTKAADVGADLVGKIEQNLNEDDPRNPATIADN
                     VGDNVGDCAGMAADVFETYAVSLIGGVLVGHLTGPEGSHAVLVYPFVLCGLAIIASVL
                     GIAWVNFVKQKATAALVGGVAVAGLISAGLFKWATGAMFSEPLAMAGKTVEAGDLYYC
                     SLIGIVMTFVVVWITNYFTSTAHAPVRRIAKSSETGHATNIIAGISVGHHATLLPVLA
                     IAASIWLCWDLAGLYGIAIAVVSMLSLSGIIISLDAFGPITDNAGGVAVMSGLPEEVR
                     AITDELDAVGNTMKAVTKGYAIASAGLAAMVLFGSYVEELKFVLKEAVIAFDLMNPKV
                     IIGMFIGGLLPYLFTAFGMDAVGSAAGAVVREVRRQIQLKPGILTGQDTPEYGQCVDI
                     VTKAALRQMILPALLPIVFVVGVAFLGKEALGGLLIGTIVTGLFVGIAMTSAGGAWDN
                     AKKFIEEGNHGGKGSFAHQAAVTGDTVGDPYKDTSGPAVNPMIKVVNVLAILVIPIFF
                     K"
     gene            complement(7907..8110)
                     /locus_tag="PLP58_07260"
     CDS             complement(7907..8110)
                     /locus_tag="PLP58_07260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007414282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ08349.1"
                     /translation="MPAPSRTPYAQFQAELEQIMLHKWLASESEGKDIGFERALNDWA
                     LNHRAAWRREQNNGQKPAPARKG"
     gene            8239..8313
                     /locus_tag="PLP58_07265"
     tRNA            8239..8313
                     /locus_tag="PLP58_07265"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:8271..8273,aa:Thr,seq:cgt)
     gene            8326..9186
                     /locus_tag="PLP58_07270"
     CDS             8326..9186
                     /locus_tag="PLP58_07270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ08350.1"
                     /translation="MTRSDASNTPAIHERRAALCRGLARLAGCAALVLGVAAVVYTVL
                     PKPSPGPAVKEEGGAPVATPQETVFESDSAREQEMLENAARFFFEAGTWHELAAICRE
                     PERVGPLIREWHQSRPVIPLRWRQLAWSQPIEEEGRRLFYARSEFEGHEPVNIIIEQS
                     GGVYRVDWESHVRHSEMDWQDFIKSRPVEPRLFRVLGSLLPGGTDPATPDRLQIRHPD
                     LSDSLIEAELIPGDPVHQRVRSQLEMGAWKNVPLTLRLCYDEKNRNNESGAVRITGVE
                     GKGWLILSRK"
     gene            9198..10472
                     /locus_tag="PLP58_07275"
     CDS             9198..10472
                     /locus_tag="PLP58_07275"
                     /inference="COORDINATES: protein motif:HMM:NF005596.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004401 - histidinol-phosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphatase HisJ family protein"
                     /protein_id="HRJ08351.1"
                     /translation="MSKTQKSKAKPAAKASPKAGKTASKAAPARKKPAPAAAKKTAPG
                     KKAAPAKKPAAAKKASTPKKNAAVKKPAPARPPAKKPAPVNKPAARKPAAVKKAPAHK
                     PASPAVTKKPAATPKPVKAAAVPAAPVVQVSEPPPPRTVSKTIKSSALYYDSHMHTPL
                     CKHAWGEPEEYAAQGIKAGLKGIIFTCHCPMPDGYWPSVRMADSEFDLYVQLVARAAS
                     AFEGRLDVCLGLESEFYPGCEPWIEKLHQRADFDFILGGLHWQAKEYLSKYEQGTIEN
                     FRRTYFEHLAQSAESGLYDCLAHPDLVKNYHPDSWCFAIIKNTVAGALDRIAKTGVSM
                     EINTSGLNKSYSEMNPGNEMLRMMAERGIPVVLGSDAHRANRVGEHFITALNNLADAG
                     YEEVGYFQKRQRVSLKISEVISSIRRAADANL"
     gene            10594..12930
                     /locus_tag="PLP58_07280"
     CDS             10594..12930
                     /locus_tag="PLP58_07280"
                     /inference="COORDINATES: protein motif:HMM:NF012787.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin domain-containing protein"
                     /protein_id="HRJ08352.1"
                     /translation="MRLHLCLVLACAQLLRAAYPLPGHSTHGEAFNEGPRQAAVLIEG
                     AGRVDFPVKTAKPMAQRFFNQGVGQLHGFWFYEAERSFRTAAALDPDCAMTYWGMAMA
                     NVNNETRAKQFTAKAAALKDKAGDSDRLWIKCLENYYKAGDKREKKQREIDFIRDLEE
                     IVHAEPKNVEAKAFLVWKTWHAKKEAPISSPQAVDALLDQIFAAAPDHPAHHYRIHLW
                     DDRKPARALESAAQCGQAAPAIAHMWHMPGHTYSKVRRFDDAVWQQEASTRVDHAYMM
                     REFILPDQIHNYAHNEEWLVRNYNELGRAKDAAALARNLIAHPRHPKHNTLDKASSSG
                     LYGRTRLLETLVKWELWEDLLALEKSPLMEPAMQEGHEAARLRAYGVAHYHLGDKDGL
                     ENRITALEKLEKKTAAEKAAKEKADKEKAEKERASAAAKTAEAASKPPAQSSPETPPA
                     KAEAAKATPAKAPAKAAAKSKSAPDEKKAETTPAQKALAELRALRAILDNHADAPRLL
                     EAARDIPKDRAARLWLRLGHQHKAAGLIKELPQDLAGQGARAEILMACGRHDEARKAF
                     DEARKLAFAMDTGLPLHHRLAALALTFGTGESWRAEPPKRAGTGKRPPLDSLGPMHWS
                     PPAAPGWEALTLDGQPWQSSALVGKNHLLIFYLGSACTHCMEQVSAFAKAAADFEKAG
                     IHLTAVTTEPMSLAGRLAEQMSDKKMPPFPVLCDPSLEAFRAFRAYDDFEQEPLHAAV
                     LVDASGRLRWLDVSWEPFTDTKFLLTESRRLLRIKKTE"
     gene            complement(12933..13070)
                     /locus_tag="PLP58_07285"
     CDS             complement(12933..13070)
                     /locus_tag="PLP58_07285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ08353.1"
                     /translation="MKQDWQSLAALVVVLTTAAAFLARASRRRWKGGGCGGGCGCRKK
                     L"
     gene            13177..13566
                     /locus_tag="PLP58_07290"
     CDS             13177..13566
                     /locus_tag="PLP58_07290"
                     /inference="COORDINATES: protein motif:HMM:NF024759.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GxxExxY protein"
                     /protein_id="HRJ08354.1"
                     /translation="MEEQDLPHLVMRACMQVHQTLGAGLSREVYEECLAVELRDMEMP
                     AERGCPLNFNYRGWQIKTPSQMDFIIAGALLVRVLAVDEVSLQERQFMETWLRLTGMK
                     MGMIVNFQVPVLRKGIHRITLKRKTAG"
     gene            complement(13879..15513)
                     /locus_tag="PLP58_07295"
     CDS             complement(13879..15513)
                     /locus_tag="PLP58_07295"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HRJ08355.1"
                     /translation="MPRLTSLIALLALPCAALAAPPNIVLVLSDDHSQPFLGCYGRTD
                     LKTPHLDRLAAEGIKFRRMFTAAPQCVPSRAAIMTGRSPVAARITRFSSPLPRDETTF
                     PEILRSEAGYFVGVCGRSYHLDGSGRAPAATDAVFDEHQMRTFTDRFDYVDATGQENV
                     PARMGEFFDRRPKDKPFFLWVNFSDPHHPWTSGKSPPDPDKIAVPGWLPDLPGVRGDL
                     SRHMGEIENCDTDFQRVLDIVKERAGLDNTLVIFMGDNGMAFPSGKGSLHDPGLNVPL
                     LAWWPGVIQPGGDSSALISGEDIAPTCLEAAGLKVPQRISGVSFLPLLKGESFPNERK
                     HIFAERGPHGSATFDLNTAASSVDYSRCVRSARYKLIYNVTPHQRYSPVDSAGDPGWK
                     EIVLAQEEKRLATEFVETWFTTPRPVYELYDLEADPNELHNLYGQKGLEDITRQLKET
                     LQKKMILDFDYLPLPIPSAPKGKAPTKARGDPKRAEMFQKLDTDKDGRLSPAEFGKNR
                     KPNEAEAWFKARDRNGDDYLDGAEYTASTVTNAPKF"
     gene            15748..17163
                     /locus_tag="PLP58_07300"
     CDS             15748..17163
                     /locus_tag="PLP58_07300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="WD40 repeat domain-containing protein"
                     /protein_id="HRJ08356.1"
                     /translation="MLAVPPCAKRILVLLLPASLLASEDDWKGASKVTTTLVDEFGRR
                     QDALLGLAASATRRGCRVAKVAGNILDGFTVRVDGKPGPRYEMIAKETPVFSEDGSSI
                     AYAVRRLGDWMWVVNGMEGPAFPELTATSFAFSADGRRHAYVGIPRFRQSTLMVDGKV
                     QAEGGWEDTMPWDAAPLFSPDGSRLAYVEARRSEKKMRVHLDGNPGPWHEGIALVTSA
                     GFGAFGVSSGELSGVAAERARPGVFQMRFSDDGKRFAYGMFAAARNGLIVDGKQMGLH
                     DAYGFDFIFSPDASDYATMMWDGTKRWVISAKRDPMEIEKIFDWSLTYSPDSQHLSFA
                     AVRDGKLAIWLDGKPAPCDIPFKDCPNWKAILFSPDSKRLAFIVRTETALHWVVDGKA
                     DPGAKFIGSNVTMDFSPDSRHYAYAATDAAGKEVRVVVDGVVRARHATIACGPVFRND
                     GVLEYLAIEETALMRYEVRLE"
     gene            complement(17835..19244)
                     /locus_tag="PLP58_07305"
     CDS             complement(17835..19244)
                     /locus_tag="PLP58_07305"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HRJ08357.1"
                     /translation="MRCLVLLFALCPLLSAFARPNIVVIVADDLGYADVTFHPQHAKE
                     IVTPNLDSLAKAGVICRQGYVTGHVCSPTRAGLMLGRYQQRLGLYTGGEAGSGVPMSE
                     KLFPQYLKPAGYATGQFGKWHLGPDLAWSPALRGFDEVFGFLGRGAHDYFKLDDPNDP
                     IYRGTEVVKETGYLTDRLGEETAAFITKHKAGPFFAYLAFNAVHAPLQAPEDEIAKFN
                     TGNKDRDARLAMGKRMDDAIGRVMTALKDGGVWENTLLFFISDNGGPLAQSADNTPLR
                     GGKHTDYEGGIRVPFLICWPSQLKPGEWQSPVTSLDIFPTTLAAAGIEVPKEARLDGQ
                     NLLPLLKGETAPASRDLFWCSGSDEGWWAVRSGDWKLVGEKDKLGLFDLSKDVSEAND
                     LAKQMPEKVAQLTKLHDAWLAEMAKPVKAGEKRYNMAAPAEKKPKKMTKEEKKKARDM
                     ERAKKRQPTTPETPEALKP"
     gene            complement(19963..20745)
                     /locus_tag="PLP58_07310"
     CDS             complement(19963..20745)
                     /locus_tag="PLP58_07310"
                     /inference="COORDINATES: protein motif:HMM:NF017885.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HRJ08358.1"
                     /translation="MSVRKPNLIKAQGEAFKLNKRYACRKPGSLDFEDVAFALNVLVI
                     HGGLKGAAARLTRNNKEGRIRVNSRIQQEGAKRFAIGHELGHWLMHESEDQIFMCTSS
                     DLRDYHQSPLEAEANYFASELLMPTGLFRPMMEKSEPDLVRISEWAGLFRASLTAATL
                     RFIRESKHDCIAASIKNGRVEWSWRKQDGRRVWLKSAQAVEPGSIAWEIQNGRAAPEQ
                     MEAVDSDAWFRHLPFKFSGEVQEQSIHMPQYGSIFSLLWITS"
     gene            complement(20732..21169)
                     /locus_tag="PLP58_07315"
     CDS             complement(20732..21169)
                     /locus_tag="PLP58_07315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ08359.1"
                     /translation="MNDQTRPRTAADVLRDLHSFIADPPLDIDSLSDDQIRSELKMRR
                     IDSTASFRAIHDLLDDHIAREELALARKNRMKKLEARSSSPSLVEGVRDKIQSLIKNL
                     NPQLAGVYWSKYQTAEEEDLQSLCEDLEALTEESTSPDGNVSP"
     gene            complement(21162..21797)
                     /locus_tag="PLP58_07320"
     CDS             complement(21162..21797)
                     /locus_tag="PLP58_07320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ08360.1"
                     /translation="MSAPDRTIAQRVKTELTQQLWDRLLLHAVDLVKRATWHGILGGP
                     SVCARDGADYVQIAILETLKGETRHWNPENCTLEQYLRGVIRSIVSNDATRAENRKFI
                     RHDPTGCSESEEVQSPLDLIPAEAPSVSDLEYEEERQSLIFEFLEQLGDDKPVQQLFE
                     AIYDGVTKRADQAARLNIPVTQVDALQKRLATRTNRFTEQKIKQRQEGSHE"
     gene            22002..23051
                     /locus_tag="PLP58_07325"
     CDS             22002..23051
                     /locus_tag="PLP58_07325"
                     /inference="COORDINATES: protein motif:HMM:NF025817.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="E2/UBC family protein"
                     /protein_id="HRJ08361.1"
                     /translation="MALVNDGNVHAPTHRVKARVLKTQAGVPEDHILVRDHESGRDVA
                     FEDDVLIDLAEGNVFMTMPRCEFSPTGNCVGRPKLAWFVDDRPEITLLAEQSGRTLRD
                     LFGLTIATRLFRDAESPQDEMIGPEKAIAFPEGPVFYSRRPGVGLTITVNKQTFGQQD
                     GVKPEMTGREIGNLVTTEPCEVKQLQKGKPEIDIPLNAKVKIEGCEEFKVIRCNVVGG
                     YEQARIDRELDVLRENGAEVDFIPGGRPAVIYRRVPTRPGYGAVAETDVLVVIPPAFP
                     GAFLDGAYLPKGSPLLGKVAGNAAQGDLQADGKVWELVSYHPHNGGGGPPWNPNRHGI
                     HSYYTEVLSWIQKAN"
     gene            23030..24514
                     /locus_tag="PLP58_07330"
     CDS             23030..24514
                     /locus_tag="PLP58_07330"
                     /inference="COORDINATES: protein motif:HMM:NF013094.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ThiF family adenylyltransferase"
                     /protein_id="HRJ08362.1"
                     /translation="MDSKSQLILPIEPAADDSQCIVRWTSDQHRTLERLLYRRYPHEE
                     WGTFFRFGYRKTSWGMVICFVDLLPPNLGDFDPKSPLVEFSPGYINRALSTFDEGDFG
                     IGFIHSHPQGCRPTPSRSDDDMDAYFASEFEKFSNGRPFVSLIASRDADHRRTFSGRC
                     FHRGHWMPVTEWLTCGGDVLRRERSLLAPSKPSDDCDDLERVTQLLGEATAPRLRNAV
                     VGIVGCSGLGTPAGHVLARSGIGEFVLVDVGFFKKSNHERNHASRASDLNHSQIPKVQ
                     LLKRLIHEIRPSAKVTCLQADVLDDGVLDQLVRCDLVLGCTDSYYARAALGDIATHFS
                     VPVLDLAVQMGSKDGALTTQTGEIARYMPGLPCPWCRNRVNAVQIRAETASGEERAQA
                     RAAAQQAQDRGEDGAPYWIGDHPQELTVGYMTSIVGSLGAGYAHNWLTGAATMPHNRM
                     QFDIGKTAFGFADDDERHSISDCSCNKCVGFADQGRAFFTVSRR"
     gene            complement(24595..25971)
                     /locus_tag="PLP58_07335"
     CDS             complement(24595..25971)
                     /locus_tag="PLP58_07335"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase"
                     /protein_id="HRJ08363.1"
                     /translation="MRLLVLLLSVCPLLAAFAKPNVLLITADDLRDFGGAFTKDFVKT
                     PHLNRLRARGTTFERAYVQYPVCNPSRSSMMAGLRAEQTGIVGNDIPLRKTMPEIVTL
                     PQLCKDAGWQSHAFGKLYHLGGGKNAEAKQRWMDAGRSWHTAQAFEATKAGRKMLEGR
                     DVTGGALKWCQWGAADGTDDDQPDGQIAAATVAMIEKLGDTPWFIGCGFMKPHDPFIA
                     PKKYFDLYPPGSLKPWSDPADMTAARKESVGFGDYGEAFAKFTTQEWQELFRAYCAGT
                     SFMDAQLGRVLDALDKHQLWENTIVVFVGDHGYHTGERQWWNKNTLFERSCRAPLIIA
                     APGMKGGQTTRSLAEFVDVYPTVADFCGLKMPHTAAGASLRPVLADPTASIKDAAFTL
                     VTRGEKLHGQSIRTARWRFTRWSDGQTELYDHETDPEELHDVSAQNADVTAELTSRIL
                     KLPPVSSR"
     gene            26296..>26488
                     /locus_tag="PLP58_07340"
     CDS             26296..>26488
                     /locus_tag="PLP58_07340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ08364.1"
                     /translation="MPLLQAGGAGGAPGQICGFLPEFARPYLGGGEPVIRRALAYQRG
                     VTLAWREAAVKARPFLAGVF"
BASE COUNT         4972 a         8416 c         7801 g         5299 t
ORIGIN      
        1 ccagtggggc attccacggt gcggctccgt taggacgggc agtcccctgc cccggcaccg
       61 gaagccgccg cgcctgcggc tgaggctcct gctgaggagg catccggccc ttccatcgaa
      121 ctcttcacaa caaacaatct gtggatgatg atcgctgccg cgctggtctt catcatgcat
      181 ctcggtttcg ccactgtgga gtcaggactc acccagtcca aaaacacggt gaacatcctc
      241 ttcaaaaaca ctttgatacc ttgtattggc ctgctgacat atgcgttgtg cggtttcaat
      301 ctgatgtatc caggtttcgc tgacgaggat cctcagttct ggttcaaatt cgccggcttt
      361 gggatcacca cggatgaggc gggagtcaca tcagcttaca atgtgggata cacttattgg
      421 accgatttcc tgttccaagc catgttcgcc gccaccgctg cgaccattgt ctcgggtgct
      481 gtggccgagc gcgtgaaact gggcactttt ctggtcttct ccacaatttt tgtcgctgtc
      541 tgctacccga tcgctggcag ttggaaatgg ggcaatggct ggctcaaccg actggacaca
      601 cctttctacg acttcgccgg ttcgacactt gtgcattcag tgggcggctg gggcgccctt
      661 gccggggtca tgcttctcgg accccgcatg ggcaaataca catccgacgg caagatcaaa
      721 cccatccttg gacacagcat gcctcttgcc accataggcg tctttctcct ctggctcggc
      781 tggttcggat tcaacggcgg ttcggttctc tctgctgatc ccggcctcgt ctctctgacg
      841 cttgtgacca cctctctcgc cgctgccgct ggcggagtct cagcggccct cgcctctgtg
      901 atcttcctca aaaaacccga cctctcgatg gcgctcaatg gcatcctggc gggcttggtg
      961 ggcattactg ctggagccga ccagatggcc gccgacacct ccatctacat cggcgccatc
     1021 gccggcgttc tggtcgtgct ctcggttctg ttcttcgaca aaatcaaaat tgacgatccc
     1081 gttggcgcca tctcggtgca tctggtttgc ggcatctggg gcacactggc cgtcggcatc
     1141 ttcggagcca aggccggcac ggctcagttg atgagccagc ttacgggtgt tgccgcctac
     1201 gcggtcttca cctttgtctt tgccttcgtc ctgttcctga tcttgaagat cgtcatggga
     1261 ctgcgtgtca gccccgagga ggaatccgaa ggcctggaca ttggcgaaca cggcaacgtg
     1321 gcctacccga acttcatcgc caatcactaa tcggaaccgg cgaactggaa tcccccgaca
     1381 tcaaactccc cggcggcata cgccggggag tttttttttg atttggcacg gcctttgcta
     1441 tccagccatg actgcggctg aggcttgtcc caagccgcaa aacatcaacc caaccaacca
     1501 cgtcgcaatg atacgcacac tgcaactttg ccgcacactc gcatgcggag tcctagccgc
     1561 caccctactg ctgagcggtc aaatccaggc ccaggaaacc cagcctgcca cggctgccac
     1621 tcccacactg gaggaactgg ccgccgatct cgctgatgtg caggcttaca tcaacaacgg
     1681 agcgcgtgtc accgaggccg gcaaatccaa aatcgccggt ccgggtccag gccacaacgc
     1741 ctggcagatg gtcagcacgg ctctggtgct gttcatgaca ctacccggtc tggccctctt
     1801 ttacggcggc ctggttcgca aaaagaacgt cctctccgtg attgcgcaat gcatgggcat
     1861 cgccggcctg gtcaccatcc tgtggtgggc ctgcggatac agtctgtcct ttggcaccgg
     1921 caatgcgtat gtcggcgact tcagctacaa attcctcaat ggcgtcgagc ctggcaatgt
     1981 cggcgcgggc tatcattgga tcagcgacag catgtggtgc atgttccagc tcaccttcgc
     2041 catcatcacg cccgcgctca tcgtaggcgc catcgccgag cgcatgaaat tcatctccgt
     2101 gctggtcttc accgccctct ggatgttcgc ggtttatttc cccttcgccc acatggtctg
     2161 ggcgggcagc ggcttcatgt gcggcccgct caatgccgac gccggcatca aggcgattga
     2221 cttcgccggc ggcacggtgg tccacatgac ctccggctgg agcgccctcg ttctctgcat
     2281 catgctgggc aggcgccgcg gtcatggcaa ggagccgatg gcccctcaca gcatggtgct
     2341 ctgcgccatc ggcacgggca tgctctgggt cggctggtat ggcttcaatg ccggctcagc
     2401 gcttggcgcc gacgccatcg cggccaatgc cttcaccacc accacgcttg ctgccgccac
     2461 cgcgggattc gtctgggcgc tggctgagtg ggtgctgcgc ggcaaaccct ccgtgctggg
     2521 tttttgctcg ggcattgtcg ccggcctcgt cgtcatcaca cccggagcgg gatttgtcac
     2581 cgccacatcc tcggtcatca tcggtgtgct cgccggaatc gtgccttttc tggcggtggc
     2641 ctatttgaaa aacgccctgg gttatgacga tgcgctggac acattcggca ttcatggcgt
     2701 gggaggcacg cttggcgcca tccttaccgg cgtgttcgcg gatgagaagg ccaactcggt
     2761 cgtcgccggc ctcaaggacg gcctcgtcat ggagcagttc aaggcggtcg gcctcaccat
     2821 tgtctggagt gtcgccgcca cggtcgtcat cacgcttgtc gtgaaaatgc tcgtcggcct
     2881 gcgcgccgat gccgaggtgg aaagcctcgg tctcgatctc gccgagcacg gcgaggccgg
     2941 ctacgagtga ttgcccgggc agcctggatt ttggctgcat cccgaccggg gagactcgcc
     3001 gtctccccgg tttttttgtt tgcaggcatt gccagccttg gtccggcctg acactggaca
     3061 tccactgccc gcgagtataa acctgcgcgc ctcacactcc gccatgtcca cgctcctcat
     3121 cgcctccggc gccttcgtgc tctacatcgt cgcctaccac acctacggcc gctggctttc
     3181 ccagaaaatc ttcaagctgg acccgcgggc cacggtgccg agcgtggagc tgaacgacga
     3241 ccgcgactat gtgccgacct caaaaggcat tgtcttcggc caccacttca ccagcatcgc
     3301 gggcacgggg ccgattgtcg gcccggcgct ggcgatcatg tggggctggg tgccggcgct
     3361 gctgtgggtg ctgtttggca gcatcttcat cggtgccatg catgatttag gcacgctcgt
     3421 tgtcagcctg cgcaaccgcg gcatgactgt gggcgaggtg gcgggccgtt tgctcaacaa
     3481 gcgcgtgcgc ctgcttttcc tcgtgctgct gcttttcgcg ctgtgggtgg tgctggccat
     3541 cttcggctgg gtcatcgcca gcgtttttgt gcagttcccc gagtccatcc tgccggtgtt
     3601 tttgcagata cccatcgcca tctggatcgg cgtgaaagtc caccgccagg gcaaaagcct
     3661 gctgtggccc agcatcatcg ccgtcagcct gatgtacgcc accgtctggc tcggcgcagg
     3721 gtgccctggc atggagtgga ccggcggcac gctcggagcc gccattcagt ccctgaatca
     3781 aagcctcgcc gcctggccgc tgtggatgtg ggtggctgtg ctgctcgcgt attgctacgc
     3841 cgccagcgtg atgccggtgt ggatcctgct ccagccgcgc gactacatca acagcctcca
     3901 gcttgtctcc agcctcgtgc tcatcgtcgt cggtctcgtc gtcgccgcgt tcatcggcac
     3961 aggaggggag aacgccgcgc cgctcgtcat ggccgcgccc gccttcaatg ccagcccgct
     4021 gaacgcgccg cccatcttcc catttttgtt tgtcaccatc gcctgcgggg cgatcagcgg
     4081 ctttcactgc ctggtcagct ccggcatgag cagcaagcag ctcaaatgcg agacggacgc
     4141 gcagttcgtc ggctacggct ccatgctgct ggagggcttc ctggccacgc tcgtcatcct
     4201 cgccgtcggc gcgggcatcg gcatgggctg gcagcagtat ccaggtctgc aaggcgcgga
     4261 gctgtggggc acggtttaca aggattggaa gctcgtcacc ggcggctccg ccatcgccgc
     4321 cttcgtcgtc ggctcggcca attttgtgca gacgctcggc atcgagcctg tcatggcgcg
     4381 tgcgctgatg ggcgtgcttg tcgccagctt tgccggcacc acgctggaca ccgccacgcg
     4441 cctgcagcgc tacgtcgtgc aggagctggc cgccaccttt gcgccgagag tctcccccac
     4501 cgccatggcc gccgaggctt acgacaccga gttcgagcgc ggcgtggtgc gcaagggctt
     4561 cagcctcaac ccgctcgtct ggctcacgaa cacgcatgga gccacgcttt tcgccgtcgg
     4621 caccgccttc ctcctcgccc tgttccccgc gccgggggcg gaatggtcat gggacaccat
     4681 tggcaagggc ggcctcattc tctggccgct ctttggcgcc acaaaccagc tcctcgccgg
     4741 tctggcgatg atggtcatca gcttctggct gctgcggcgc ggcatgccca cctggtttgc
     4801 cgccacaccc atggccttca tgctcgccat gcccgcctgg gcgctttatg tggacgtgca
     4861 gcgctggctg gccggcggaa gctgggtgct cgtgggcgtc ggcctcatca tgctggccat
     4921 cgaggtctgg atgatcatcg aagcctgcct gctgtggccg aaggtgcggg gtgtgctgga
     4981 agctccgctg ccgccgctgc cggccaggac cggcgcgcaa accgcctgag ccggccatgc
     5041 gctgcccgag ctgccgccat cccgccgccg agcatctggc ggtgtgcggg cattgcggat
     5101 tctgcctgga ggctgtggac cgcgccctcg gcgcgccacc cgccatgaca gcgccgctga
     5161 ctgatgccgc cgccattctt acgagacgtg aaaaaaccgc cgtgcttggc gctctgaatg
     5221 ccttctccag tcgccatccg cagctcgttc cagtctgcgt gctggcgaat gtgccgcccg
     5281 gtgtcccggt ggaaatttac actttttgga tccacaaccg cgcgggcttt ttcagcctgt
     5341 ccgaaagcgg cgggcgcaat cacggcctgc ttctcgtgct cgatccgcag gcaaatagcg
     5401 ccgccgccat gcccggatac ggactcgaac ctttcattgg cgaggacgac ctagctcccg
     5461 ccctggcagc cgcccgcctt tccctggaca gcggtgaccc ggcgcgtgcc gccggggtgt
     5521 ttttccggga gatgagcgcg cgcctcctca tcatctctga gaatctcccc cgcgccttcg
     5581 gctacgacga gacccgcccc tggctagaca gtgaaggcgg cgatgactgg accattccct
     5641 cgcatgaggc tgcggatgtg tattgagcct gcctcgtcat tccgcgcgca gaaactcagg
     5701 acgggagtgc ccgcatggct cccgatcctg tcttggcggt ggcacaaaag agcaggaaaa
     5761 gagtcttgcc agatcaaagc gagccgctag actcggcggc ttctcaaacc ggcctcaacc
     5821 agccggtctt ttcttttata ctcccgatga attcgttgct tctcgatcaa ggcgtccttg
     5881 tgtccatcgc cctcgccgtg gcgggcttgg tgattgccgt gctgctcatc cgccagattc
     5941 tggcgtcctc cgcgggcaat gaaaagatgg cgttgatcgg cggcgccatc caggaggggg
     6001 cgaaggctta tctgaaccgg caaatccgcg ccgtgggcct catcgccatc atcattttct
     6061 tcgtcgtttg gtaccttcgc gacggcatga cctcggcggg cttcgtggtc ggcgcggtct
     6121 gctcgatggt ggccggctac atcggcatga tgatcgccgt gcgggcgaat gtgcgcaccg
     6181 ctcaagccgc cagcgtcagc tcccacgcgg cgctgaaggt ggccttcaac ggaggagccg
     6241 tcaccggtct gctggtggtg gcgctggggc tgctctccgt cggcatgttc tacctcgtca
     6301 ttgaaaaaat ggccggcgtc cgggcggcga ttgactcgct ggtcggcctg gcccttggca
     6361 gctcgctgat ctccgtcttt gcgcgtctag gcggcggcat ttacaccaag gcggcagacg
     6421 tgggcgcgga cttggtcggc aaaatcgagc agaacctcaa tgaggacgat ccgcgcaacc
     6481 ccgccaccat cgccgacaat gtcggggaca atgtcggcga ttgcgcgggc atggcggcgg
     6541 atgtttttga aacctacgcg gtgagcctca tcggcggcgt gcttgtcggg catctcaccg
     6601 ggccggaggg cagccacgcg gtgctcgtct atccctttgt gctttgcggc ctggccatca
     6661 tcgcctccgt gctcggcatc gcctgggtca attttgtgaa gcagaaagcc acggcggcgc
     6721 tcgtcggcgg cgtggccgtg gcgggcctca tttccgccgg tcttttcaaa tgggcgacag
     6781 gcgccatgtt cagcgagcct ctcgccatgg cgggcaaaac cgtcgaggcc ggcgatctct
     6841 attactgctc gctcatcggc atcgtcatga ccttcgtggt ggtgtggatc acgaattact
     6901 tcacctccac cgcgcacgcg ccggtgcgcc gcattgccaa gtcctccgag accggccacg
     6961 ccacaaacat catcgccgga atcagcgtcg gccaccacgc caccctcctg ccggtgcttg
     7021 ccatcgccgc ctcgatctgg ctgtgctggg atctggcggg cctctatggc attgccatcg
     7081 ccgtcgtctc catgctcagc ctctcgggca tcatcatctc cctcgacgcc tttggtccca
     7141 tcaccgacaa cgcgggcggc gtggccgtca tgtccggcct gcctgaggag gtgcgcgcca
     7201 tcactgacga gctcgacgcc gtgggcaaca cgatgaaagc cgtcaccaag ggctacgcca
     7261 tcgcctccgc tggtttggcc gccatggtgc tcttcggctc ctatgtcgaa gagttgaagt
     7321 tcgtgctcaa ggaggccgtc atcgcctttg acctgatgaa cccgaaagtc atcatcggca
     7381 tgttcatcgg cggcctgctg ccctacctct tcaccgcctt cggcatggac gccgtgggca
     7441 gcgccgccgg cgccgtcgtg cgcgaggtgc gccgccagat ccagctcaag ccaggcatcc
     7501 tcaccggcca ggacactccc gaatacggcc agtgcgtgga catcgtcacg aaagccgccc
     7561 tgcgccagat gatcctcccc gccctgcttc ccattgtgtt cgtggtcggc gtcgccttcc
     7621 tcggcaagga ggccctcggc gggctgctca tcggcaccat cgtcaccggc ttgttcgtag
     7681 gcattgccat gacctccgcc ggcggcgcat gggacaatgc gaagaagttc atcgaggaag
     7741 gcaaccacgg cggcaagggc agcttcgccc accaggctgc cgtgaccgga gacaccgttg
     7801 gcgaccccta caaggacacc tccggcccgg cggtgaaccc gatgatcaag gtggtcaacg
     7861 tgctggccat cctcgtcatc ccgatctttt tcaaataggc ggcctgctag ccctttcggg
     7921 cgggcgccgg cttttgacca ttgttctgct ccctccgcca tgcggccctg tggtttagcg
     7981 cccagtcatt gagcgcgcgc tcaaagccga tgtcttttcc ctcgctctcg ctggccagcc
     8041 atttgtggag catgatctgc tcgagctcgg cctggaactg ggcgtaggga gtgcgtgacg
     8101 gtgctggcat ggccgcgctg ataaagcggg cgcgcttgag tttgcaagcc gccgctccgc
     8161 aaaatcgccg ctttggcaga aacaaaatga caaatccccc gcgccgtgcc agacttcgcg
     8221 cccaaccgat ccgatactgc cggtgtagct cagtggtaga gcatctcatt cgtaatgaga
     8281 gggtcgcggg ttcaaatccc gcccccggct ccagtgtgga aagcgatgac ccgaagcgac
     8341 gcatcgaaca cacccgccat tcatgagcgg cgcgccgcgc tttgcagggg cctcgcgcgc
     8401 ctcgccgggt gcgccgccct ggttcttgga gtcgccgcag tcgtttacac cgtcctccca
     8461 aagccctccc ccggtccagc agtgaaagag gagggcgggg cacctgttgc caccccgcag
     8521 gagacagttt ttgagtccga ttccgcccgt gagcaggaga tgctggaaaa tgcggcgcgc
     8581 ttctttttcg aggccggcac ctggcatgaa ctggccgcca tttgccgcga accggagagg
     8641 gtggggccgc tcattcgtga gtggcatcaa agccgccccg tcattcccct gcgctggcgg
     8701 cagctcgcct ggtcgcagcc catcgaggag gaaggcaggc ggttgttcta cgcgcgctcc
     8761 gagttcgaag ggcacgagcc ggtcaatatc atcatcgagc agagcggggg agtctatcgc
     8821 gtggactggg agagccacgt ccgtcacagc gagatggact ggcaggactt catcaaaagc
     8881 cgcccggtgg aaccccggct gttccgcgtc ctcggctccc tgctgccagg aggcacggac
     8941 ccggcgaccc cggacaggct gcagatccgc caccccgacc tctccgattc cctgatcgaa
     9001 gccgaactga tccccggcga tcccgtccac cagcgcgttc gcagccagtt ggaaatgggt
     9061 gcctggaaaa atgtaccatt gacattgcgg ctgtgttatg acgaaaagaa caggaacaat
     9121 gagtccggcg cggttcgcat cactggtgtc gaaggcaagg gctggttgat cctgagccgc
     9181 aaataggaga cacgcccgtg agcaagaccc aaaaatccaa agccaaaccc gccgccaaag
     9241 catcccccaa ggcgggaaaa accgcgtcca aagccgctcc cgccaggaaa aagccagctc
     9301 cggccgccgc caaaaagacc gctcccggca aaaaagccgc gcctgccaaa aagcccgccg
     9361 ccgcaaaaaa agcctccacg cccaaaaaaa acgccgcagt gaaaaagccc gctccggcca
     9421 ggcctcccgc caagaagccg gcgccggtca ataagcccgc cgccaggaaa ccggccgccg
     9481 tcaaaaaagc cccggcccac aagccagcca gcccggcagt caccaaaaaa cccgccgcca
     9541 cgcccaagcc agtcaaagcc gccgccgtcc cggccgctcc agttgtgcag gtgagcgagc
     9601 cgcctccgcc ccggacggtt tccaaaacca tcaaatccag cgcgctctat tacgactccc
     9661 acatgcatac cccgttgtgc aaacacgcct ggggcgagcc ggaggagtat gccgcgcagg
     9721 gcatcaaggc cggactcaag gggatcatct tcacctgcca ctgccccatg cccgacggtt
     9781 actggcccag cgtgcgcatg gccgactccg agttcgatct ttatgtccag ctcgtcgcgc
     9841 gcgccgcctc cgcctttgaa ggcaggctcg atgtctgcct cggcctggag agcgagttct
     9901 accccggctg cgaaccctgg atcgaaaagc tccaccagcg cgctgatttc gacttcatcc
     9961 tcggcggtct ccattggcag gccaaggagt acctctccaa atatgagcag ggcaccatcg
    10021 agaacttccg ccgcacctac ttcgagcacc tcgcccagag cgccgaatcc ggcctttacg
    10081 actgcctggc tcaccctgac ctggtgaaaa actaccaccc ggacagttgg tgcttcgcca
    10141 tcatcaaaaa caccgtcgcg ggcgcgctcg accgcattgc caaaaccggc gtctccatgg
    10201 agatcaacac ctccggcctc aacaagagct actccgagat gaatccaggc aatgaaatgc
    10261 tgcgcatgat ggccgagcgc ggcatacccg tcgtcctcgg ctcggacgcc caccgcgcca
    10321 accgtgtcgg cgagcacttc atcaccgccc tgaataatct cgcggatgca ggctacgagg
    10381 aggttggcta cttccagaag cgccagcgcg tttccctcaa gatcagcgag gtcatctcca
    10441 gcatccgccg cgccgcagac gccaatcttt gaggcggctg agacttcctc ccgccgaaaa
    10501 aatacgcgcg gttgagcttc cagcacggct gccgtgaaga aacaccgccc gcatgacaga
    10561 ttcccgtcct tgagggcttt cttgtctccg tccatgcgtc ttcatctctg cctcgtcctt
    10621 gcctgcgccc agctcctccg cgccgcctat ccactgcccg gccactccac gcacggggag
    10681 gcattcaatg aaggcccgcg ccaggcggcg gtgctcattg agggcgcggg gcgggtggat
    10741 ttcccggtga agaccgccaa gccgatggca cagcgctttt tcaaccaggg cgttgggcag
    10801 ctccacggct tctggtttta cgaggccgag cgctccttcc gcacggccgc cgcgctcgat
    10861 cccgactgcg ccatgaccta ctggggcatg gccatggcga atgtgaacaa cgaaacgcgc
    10921 gcgaaacagt tcaccgccaa agccgccgcc ctcaaggaca aggccggcga ctccgaccgg
    10981 ctctggatca agtgcctgga gaactattac aaagccggtg acaagcggga gaaaaagcag
    11041 cgcgagatcg acttcatccg cgacctggag gagatcgtcc acgccgagcc gaagaatgtt
    11101 gaggcaaaag ccttcctcgt ctggaagacc tggcacgcca aaaaggaggc gcccatttcc
    11161 agcccgcagg ccgtggacgc gctgctcgat caaatcttcg ccgccgctcc cgaccacccc
    11221 gcgcaccatt accgcatcca cctgtgggac gaccgcaagc ccgcccgtgc cctggagtcc
    11281 gccgcgcaat gcgggcaggc cgcgcccgcc atcgcccaca tgtggcacat gccggggcac
    11341 acctactcga aggtgcgccg ttttgacgac gccgtctggc agcaggaggc cagcacgcgc
    11401 gtggaccacg cctacatgat gcgcgagttc atcctgcccg accagatcca caactacgcc
    11461 cacaatgagg agtggctcgt gcgcaactac aacgaactcg gccgcgccaa ggatgccgcc
    11521 gccctcgcgc gcaacctcat tgcccacccc cgccatccca agcacaacac cctcgacaaa
    11581 gccagcagct ccggcttata cgggcgcacc cggctgcttg agaccctcgt caaatgggag
    11641 ctgtgggagg acctgctcgc cttggagaaa agcccgctca tggaacccgc catgcaggag
    11701 ggccacgaag ccgcgcgtct gcgcgcctat ggcgtggcgc attatcacct cggtgacaaa
    11761 gacggactcg aaaaccgcat caccgccctg gagaaactgg agaaaaagac cgctgcagaa
    11821 aaggccgcca aagaaaaggc tgacaaggaa aaagccgaaa aggagcgcgc ctcagcagcc
    11881 gccaagaccg ccgaagccgc cagcaaaccc ccggcccaat catccccgga aaccccgccc
    11941 gccaaagctg aagctgccaa agccacccct gccaaagctc cagccaaagc cgccgccaaa
    12001 agcaaaagcg cgccggatga aaagaaagcc gagaccaccc ccgcgcagaa agccctggcc
    12061 gagttgcgcg ccctgcgcgc catcctcgac aaccatgcgg acgcgcccag gctgctcgaa
    12121 gccgccaggg acattcccaa agaccgtgcc gcgcgtcttt ggctccgcct tggccaccag
    12181 cacaaagccg ccggcctcat caaagaactc ccccaggacc tggccggaca aggcgcgcgc
    12241 gcggagatcc tcatggcttg cggacgccat gacgaggcgc gcaaggcctt cgacgaagcg
    12301 cgcaagctgg cctttgccat ggacaccggc ctgcctcttc accaccgcct cgccgcgctc
    12361 gccctcacct ttggcaccgg cgaaagctgg cgcgccgaac cgccaaaacg cgccggcacc
    12421 ggcaaacgcc cgccactgga cagtctcggc cccatgcact ggagtccgcc cgccgcgccc
    12481 ggctgggagg cgctgaccct ggacgggcag ccctggcaga gcagcgccct ggtgggaaaa
    12541 aatcacctgc tcatcttcta cctcggctcc gcctgcaccc actgcatgga gcaggtgagc
    12601 gcctttgcca aagccgcggc ggatttcgaa aaagccggca tccacctcac cgctgtcacc
    12661 accgagccca tgtccctggc cggcaggctc gccgagcaga tgagcgacaa aaaaatgccc
    12721 cctttccccg ttttgtgtga tccctcactc gaagccttca gagccttccg cgcctatgac
    12781 gacttcgagc aggaaccgct ccacgccgcc gtgctcgtgg atgccagcgg ccgcctgcgc
    12841 tggctggatg tgagctggga acccttcacc gacaccaagt tcctcctcac cgagtcccgc
    12901 cgcctcctgc gcatcaagaa gaccgagtaa gctcacagct tcttcctgca gccgcacccg
    12961 ccgccgcagc cgccgccctt ccagcgccgc cgcgacgcgc gcgccaggaa agccgccgcc
    13021 gtggtcaaca ccacaaccag cgccgccagg gactgccaat cttgtttcat gccggtcatc
    13081 tatagcctct cggtgcgttc atcaatcccg catccgttgt actggcaaga atccctgttg
    13141 aaatcccgac acctggattt ataaccgcca aattccatgg aagagcaaga tctcccccac
    13201 ctcgtgatgc gcgcctgcat gcaggtgcat cagactctcg gagcgggcct ttcacgcgag
    13261 gtttatgagg aatgcctcgc cgtcgaactc agggacatgg agatgcccgc cgagcgtggc
    13321 tgtccgctga acttcaacta ccgcggatgg cagatcaaaa ccccctcgca gatggacttc
    13381 atcatcgcag gagcgctcct cgtccgcgtt ctcgccgtgg acgaggtcag cctccaggag
    13441 cgccagttca tggagacctg gctgcgcctc accggcatga agatgggcat gatcgtcaac
    13501 ttccaagtcc ccgtcctgcg caaaggcatc caccgcatca ccctcaaacg caaaaccgcg
    13561 ggctgagagc gctgtcttca tggggcgcgg gcggcgccgt ggcgcgcgct gggcacagtc
    13621 ctaggcagga aattcctcaa aatcaaaagg gcaccccgca gccatgccct tcgatcccaa
    13681 tcttcccaag aaactcaaag cctcggacgt cgcgcggatg agcgacaact caacggcctc
    13741 aataatctca tggtcgccgt gtcggccatc accgccgagg tcgtggactc ggtgagccga
    13801 tgtcgcctgg gcgctcgccc catgatgtcg gagggcccac ctccagcagt ccaccatgga
    13861 tgcggctacg agcggtgatc agaattttgg cgcgttggtg acggtgctgg cggtgtattc
    13921 ggctccatca agatagtcat cgccattgcg gtcgcgggct ttgaaccagg cctcggcttc
    13981 gttgggcttg cggtttttgc caaactccgc cgggctcagc ctgccgtcct tgtcggtgtc
    14041 gagcttctgg aacatctccg cgcgtttggg atcgcccctg gctttggttg gagccttgcc
    14101 cttgggagcg gaggggatgg gcagcggcag gtagtcgaag tcgaggatca tctttttctg
    14161 gagcgtttcc ttgagctgcc gcgtgatgtc ttcgaggccc ttctggccat aaagattgtg
    14221 cagttcattc gggtctgcct ccagatcgta gagctcatag accggacggg gcgtggtgaa
    14281 ccaggtctcc acaaactccg tggccagccg cttttcctcc tgcgccagca cgatctcctt
    14341 ccacccggga tcgcccgcgc tgtccacggg cgagtagcgc tggtgcggcg tgacgttgta
    14401 gatgagcttg tagcgggcgc tgcgcacgca gcggctgtag tccacgctgc tggcggcggt
    14461 gttcaggtca aaggtggcgc tgccgtgcgg tccgcgctcg gcgaagatgt gcttgcgctc
    14521 attcgggaag gattcgccct tcagcagcgg cagaaagctc acgccgctga tgcgctgcgg
    14581 cactttcagg ccagccgctt cgaggcaagt cggcgcgatg tcctcgcccg agatcagcgc
    14641 actggagtcg ccgcccggct ggatgacgcc cggccaccag gcgagcagcg gcacattgag
    14701 gccgggatcg tgcagcgagc ccttgccgct ggggaaggcc atgccgttgt cgcccatgaa
    14761 aatcacgagt gtgttgtcga ggccggcgcg ctctttgacg atgtcgagca cgcgctgaaa
    14821 gtcggtgtcg cagttttcga tctcgcccat gtggcgcgag agatcaccgc gaacgccggg
    14881 cagatcaggc agccagccag ggacggcgat tttgtcagga tcaggcggcg atttgccgct
    14941 ggtccaggga tggtgcggat cgctgaagtt cacccagagg aagaagggct tgtccttcgg
    15001 gcgccggtca aagaactcgc ccatcctggc cggcacgttt tcctggccgg tggcgtccac
    15061 atagtcaaag cggtctgtga aagtgcgcat ctggtgctca tcaaagaccg catccgtggc
    15121 ggcgggcgcg cggccggagc cgtcgaggtg gtagctgcgc ccgcaaacgc cgacgaagta
    15181 gccagcctca cttcggagaa tttccgggaa cgtggtttca tcgcgcggca gcggcgaaga
    15241 gaagcgcgtg atgcgcgccg ccaccggcga gcggccggtc atgatggcag cgcgggaggg
    15301 cacgcactgc ggcgcggcgg tgaacatgcg gcggaacttg atgccctccg ccgccaggcg
    15361 gtcgaggtgg ggcgtcttca ggtcggtgcg tccatagcag cccaggaaag gctggctgtg
    15421 gtcgtccgaa agcacgagca cgatgttggg cggcgcggcg agggcggcgc agggaagcgc
    15481 cagcagagcg atgagggagg tcagacgggg catgatgaaa cggaaagtcc gcctcttgga
    15541 acggcgggct gcggccatct ttgcttcgga acacagtatg ccgggaaatt gcattggagg
    15601 gaatgaaggg ggatgagtct taatacatgg cattttcctg cccgccagca ataccaagac
    15661 cagcgtcact atgagtacgc cttgatggtc ttccgaggag ctggctttga gcttgagaag
    15721 gttagtgtcc cgaggtaaac ttaccttatg ttagccgtcc caccctgcgc gaaacggatt
    15781 cttgttcttc tcctgcctgc cagcttgcta gccagtgagg atgactggaa aggagcgtca
    15841 aaggtcacca ccactctagt ggacgagttt ggtcgtcggc aggacgctct cttggggctt
    15901 gcagcttccg ccacacgccg tggctgtcgc gtcgccaagg tggcaggcaa catcctggat
    15961 ggctttaccg tgcgtgtgga tggcaaaccc gggccgcgtt acgaaatgat tgccaaagaa
    16021 acgcccgtct ttagtgaaga tggcagttcc atcgcctatg ctgtccggcg tctcggcgac
    16081 tggatgtggg tggtgaatgg aatggagggg cctgcctttc ccgaactcac cgccacttcc
    16141 ttcgccttta gtgccgatgg gcgccgccat gcctatgtgg gtattccacg cttccgccag
    16201 tccaccctca tggtcgatgg caaagtgcag gctgagggtg gctgggagga caccatgccc
    16261 tgggatgcgg ctccgctctt cagcccagat ggttcgcggc tggcctatgt tgaggcgcgt
    16321 cgtagtgaaa aaaagatgcg ggtgcattta gatggcaatc cgggtccgtg gcacgaaggc
    16381 attgccctcg tcacatccgc agggttcgga gcctttggcg tttccagcgg agaactgagc
    16441 ggtgtggcgg cagagcgtgc gcggccaggt gtctttcaaa tgcggttctc tgatgatggc
    16501 aaacgcttcg cctatggcat gtttgcagct gcgcggaatg gcttgatagt ggacggcaag
    16561 cagatgggcc tgcatgacgc ctacggcttc gacttcatct tctcccccga tgccagcgat
    16621 tacgccacca tgatgtggga cggcacgaag cgatgggtga tctccgccaa acgcgatccg
    16681 atggagatcg aaaaaatctt cgactggagc ctcacctaca gccccgacag ccagcatctc
    16741 tcctttgctg ctgtgcgcga tggcaaactg gccatctggc tggatggcaa acccgcgccc
    16801 tgcgacatcc ctttcaaaga ctgcccgaat tggaaagcca tcctctttag cccagactca
    16861 aagcggcttg ccttcatcgt gcgtactgag accgcattgc actgggtcgt ggatggcaag
    16921 gccgatcccg gcgccaagtt catcggctcc aatgtgacca tggatttcag cccggacagc
    16981 aggcactacg cctatgcggc gacggatgcg gctggcaaag aagtgcgcgt ggtggttgat
    17041 ggcgtggtga gggcaagaca cgcgacgatt gcatgcggcc ccgtctttcg aaacgacggc
    17101 gtgctggagt acctcgccat cgaagaaaca gccctgatga gatatgaagt gcggctcgaa
    17161 taaaacatca gagcttcagg atatctgcac tagacagatc gttagcccta gcccaaactc
    17221 atgcctgtca tccatccgcg tctggtcctc ccaacactgt tgctggcatc atgtgccacc
    17281 gaaacacgtc ccggacgctc ccccgatgat acaacgaagc cctcaaagat catccgagcc
    17341 tttcgtcgtc caattgatca tggaaaagac ccatggaata gcgcaggtgt ctggcgtcgg
    17401 atcatgaaca acccaccctc gcatgttccg gcggaccttt ctcgcaacca ccccctcgat
    17461 gaagggcatg ggcagtggtt cgtggaccgc aatgacggct ggcggttctt cgttcccaag
    17521 agtggcacga ccaaatactc ggagaaactc ctccttggcg aggtcgacaa agtcactaac
    17581 agcatgactg ccagggctca ttcgagacaa aatgtgtgtg tgactgtgtt gtacatgctg
    17641 gctgcctttg tgtgcgcgcc actggctgtg atgagcgggt ggagctagcc tcatgtcctc
    17701 tggcaatttc aacatgcaac gagtgtccgc gccgtaagca tcacccggaa aatcgcgctt
    17761 agcctcgcat gctgggtgct cgagcacctg cggttagatg gcaaaggcag gagcatcgag
    17821 ataggatgcg ggagtcatgg tttgagtgct tcgggagttt cgggcgtggt gggctggcgt
    17881 tttttggcgc gctccatgtc acgagccttt ttcttttctt ccttggtcat cttcttcggc
    17941 ttcttctcgg cgggcgcggc catgttgtag cgcttctcac cagctttgac gggcttcgcc
    18001 atctccgcga gccacgcatc gtgcagcttc gtcagctgcg ccaccttctc gggcatttgc
    18061 ttggcgaggt cgttggcctc cgacacgtcc ttgctcagat caaagaggcc cagcttgtcc
    18121 ttttcgccca cgagcttcca atcgcccgaa cgcaccgccc accagccctc atcactgccg
    18181 gagcaccaaa acaaatcgcg cgatgccggt gcggtttcgc ctttcagcaa cggcagcagg
    18241 ttctgaccat cgagacgagc ctccttcggc acttcgatgc ccgccgccgc gagcgtcgtg
    18301 ggaaaaatat cgagcgatgt caccggcgac tgccattcgc ctggcttgag ctgcgacggc
    18361 cagcagatca gaaacggcac gcggatgccg ccttcgtagt cggtgtgctt gccaccgcgc
    18421 agcggcgtgt tgtcggcgct ttgcgccagc ggaccgccgt tgtcgctgat gaagaaaagc
    18481 agcgtgtttt cccatacgcc gccgtccttg agcgcggtca tcacacgacc gatcgcgtca
    18541 tccatgcgct tgcccatcgc gagtcgggca tcgcggtctt tgttgcccgt gttgaacttc
    18601 gcgatctcgt cctccggtgc ctgcaacggc gcgtgcaccg cattgaaggc caggtaagca
    18661 aagaacggcc ccgccttgtg cttcgtgatg aacgccgccg tctcctcgcc gagtcgatcc
    18721 gtcagataac ccgtctcctt caccacctcc gtgccgcgat agatcggatc attcgggtca
    18781 tcgagcttga agtagtcatg cgccccgcga ccgagaaagc cgaacacctc atcaaacccg
    18841 cgcagggccg gactccacgc cagatccggc cccaggtgcc atttgccaaa ctgccccgtc
    18901 gcatagcccg caggcttcaa atactgcggg aagagcttct cgctcatcgg cacgccactg
    18961 cctgcttcgc cacccgtgta gagccccaac cgctgctgat agcgccccag catcagcccc
    19021 gcccgcgtcg gcgagcacac atgccccgtc acatacccct gccggcaaat cacgcccgct
    19081 ttggccagcg aatcgagatt cggcgtcacg atctccttcg catgctgcgg atgaaacgtc
    19141 acatccgcat accccaggtc atccgcgacg atgacgacga tgttgggcct cgcgaaagcg
    19201 gagagcagag ggcaaagggc gaagagaaga acgagacaac gcatggtcgc gctgaaacgt
    19261 caggcttcgg cggaacttca caagaatgcc gaacagcgcc gcgaagggca cagaaaagcg
    19321 cgagcggatt atcgcgccgg tggtggagtg atagtttgaa tgtaatcgaa ggccctcagc
    19381 atggcttctt tctcgtgcgc gtaaaagcgg cccccttctt tgccgtcaat ccatagccga
    19441 aaccgtccac tctttgatgc cacagttacc cgatgcccat ttagtttgat ggttttgcca
    19501 ccgtagcgtg ttgaagacca gccctttcga gttgggaatc tgcttcgtct ggctgccttg
    19561 ttccgcagga ttctttcctg ccgttgaggt tcaacatagt cgttggtaag ccgacaggcg
    19621 cagatgcagc caacgcggat gggatcgcaa tagtctgggt ggtttagcaa gtgaacgaac
    19681 ctgatttgct catgatcaca aaactgacag tcctcatact cgccgtatga caacccctcg
    19741 tcctcgtgaa ggtcaatcac ttgttcgagt ctccaccctt tgtggggaac gctttctttg
    19801 cggcataaca tcatgaaatt ctacgcctgc gatggtgaaa ctctacctcc tgggcgtttg
    19861 gtgagagcag gaaggtagct aggaaggccg ggcgagattt gtgggcagtg tcggatcgtg
    19921 gaagcaacga gcgcactcaa tgaagcaaag cgccgctacc ggtcacgacg taatccacaa
    19981 gagactgaag atggagccat actgcggcat atgaattgat tgctcttgaa cttcaccaga
    20041 gaacttgaac ggcaaatgcc tgaaccacgc atccgaatcg accgcttcca tctgttctgg
    20101 cgcggcgcgg ccattctgaa tctcccatgc gattgaaccc ggttcgactg cctgtgcact
    20161 tttcaaccag acccgtctgc catcttgctt gcgccaagac cattcaaccc tgccgttctt
    20221 gatactggca gcaatacagt cgtgtttcga ttcccgaatg aaacgaagtg ttgcggccgt
    20281 gagcgaagca cgaaagaggc cggcccattc actgattcgg accaagtctg gctctgattt
    20341 ttccatcatc ggtctgaaca agccagtagg catcagaagc tccgaggcga agtaatttgc
    20401 ctctgcttca agcggactct gatggtaatc tctcaagtcg gaggatgtgc acataaagat
    20461 ttggtcctct gactcatgca tcagccagtg cccaagctca tgtccgattg cgaaacgctt
    20521 ggctccttcc tgctgtatcc gtgagttcac gcggatgcgt ccttctttgt tgtttcgagt
    20581 aaggcgtgct gccgcccctt tgagtccgcc atgaatcaca agcacattta gggcaaatgc
    20641 aacatcctca aaatcaagcg atcccggctt gcggcaggcg taacgcttgt tgagcttgaa
    20701 cgcctcgccc tgcgccttga tcaagtttgg cttacggact gacattgccg tcagggcttg
    20761 tggattcctc tgtcaacgcc tctaagtctt cacagagcga ctggagatct tcttcttcgg
    20821 cagtttgata tttgctccag tagacgccag cgagctgcgg attgaggttc tttatcaggc
    20881 tctgaatctt atcgcgaacg ccctctacca gagacgggga tgaactcctc gcttctagct
    20941 tcttcatgcg atttttcctc gccagagcga gttcttccct ggcaatgtga tcatccaaca
    21001 gatcatgaat ggccctgaag gaagccgtag aatcaatgcg ccgcattttc agttccgacc
    21061 tgatctggtc gtccgaaagc gagtcaatat cgagtggtgg atcggctatg aaactgtgaa
    21121 ggtctcttag cacgtccgca gcggtgcgag gtcgtgtttg atcattcatg ggaaccctcc
    21181 tgtcgttgtt tgattttttg ctcggtgaat ctgtttgttc gggtggctaa tcgcttttga
    21241 agagcatcca cttgagtgac tgggatattg agccgagcag cttggtcggc acgtttggtg
    21301 actccgtcgt agattgcttc aaacaactgc tggacgggct tgtcatcgcc caactgctcc
    21361 aagaactcga aaatcaagct ctgacgctct tcctcgtatt ctagatcgga tacgctaggt
    21421 gcttcggcag ggatcaaatc aagcggcgac tgcacttcct cactctcact gcaacctgtt
    21481 gggtcgtgtc ttatgaactt tcgattttca gcacgagtcg catcgttcga aactatgctt
    21541 cggatgactc ctctcaaata ttgctcaaga gtgcagtttt ccgggttcca gtgccgcgtt
    21601 tctcccttca atgtctctaa gattgcgatt tgaacgtaat cagccccatc tcgagcgcac
    21661 acagacggtc cgccaagtat tccgtgccaa gtagctcgct tcactagatc aactgcatgt
    21721 agcagcagtc gatcccagag ctgctgggtc agctctgtct ttacgcgttg ggcgatagtt
    21781 ctgtcgggcg cgctcatggg cgtggaagtt gcactctgtc acaagcttct gctgaaagtc
    21841 tgggggaatt cgcggctgtc ggcagaattt ttttggggca tccgcatatt ttcaggccaa
    21901 tccagcaatg gaggatgatg caaacaacgc agcaccccca aacaagcctc agcaccgctg
    21961 agggaaacca cacgcaaaac tgccaacccg cgtccaagtg gatggccctc gtgaacgacg
    22021 gaaacgttca cgcaccgacc caccgggtga aggcccgggt gctcaaaacc caagcgggtg
    22081 ttcccgaaga ccacatcctc gtgcgtgacc atgagtcggg ccgtgacgtg gcttttgagg
    22141 acgatgtact catcgacctc gccgagggca acgtcttcat gaccatgccg cgttgcgagt
    22201 tttccccgac agggaactgt gtgggccgtc cgaagctggc gtggtttgtg gatgaccgtc
    22261 ctgaaatcac cctcctggcg gagcagagcg ggcggacgct gcgcgatctc tttgggctca
    22321 cgattgccac ccggctgttt cgtgatgccg agtcaccgca agatgagatg attggccctg
    22381 aaaaggccat cgcttttccc gaaggtccgg ttttctattc tcgccgtccg ggagtcggcc
    22441 tcacgatcac cgtgaacaag cagacctttg gccagcaaga cggggtgaaa cccgaaatga
    22501 ccggacggga gattggcaac ctcgtgacga ctgagccgtg cgaggtgaaa caacttcaga
    22561 agggcaagcc tgagattgac atcccgctga atgccaaggt aaagatcgaa ggctgtgagg
    22621 agttcaaggt catccgatgc aacgtggtgg gtggctatga acaggcgcgg atcgaccgtg
    22681 aactcgatgt gcttcgcgaa aatggtgccg aggtcgattt catccctggc ggacgcccgg
    22741 cggtcattta ccgccgggtg cccacccgcc cgggttacgg cgctgtggct gaaactgatg
    22801 tcttggtggt cattccgcct gcattccctg gcgccttcct tgatggcgct tatttgccga
    22861 agggctcgcc gcttctcggc aaggtcgcag gcaacgctgc acagggagac cttcaggcgg
    22921 atgggaaagt ctgggaactg gtaagctacc acccgcacaa tggaggaggt ggcccaccct
    22981 ggaacccaaa tcgacacgga attcacagct attacacgga ggtgctgtca tggattcaaa
    23041 aagccaactg atcctgccga ttgaaccagc agcggacgat agccagtgca tcgtccgctg
    23101 gacctctgat caacaccgaa cgctggagcg gctcctttac cgccgctacc cgcatgagga
    23161 gtggggaacc ttctttcggt ttggctaccg gaagacgtct tgggggatgg tgatctgctt
    23221 tgttgatctt ctgcctccga acctgggtga ctttgatccg aagagcccgc tcgtcgagtt
    23281 ttcgccgggg tatatcaacc gcgcattgag cacgtttgat gagggcgact tcggtatcgg
    23341 cttcatccac tctcatcctc aaggatgcag acccacaccg agccggtccg acgacgacat
    23401 ggatgcctac ttcgcttccg agtttgagaa gttcagcaac ggcaggcctt ttgtgagcct
    23461 catcgcctcg cgagacgccg accaccgaag gactttcagc gggcgctgct tccatcgcgg
    23521 ccattggatg ccagtcaccg aatggctgac gtgtggcggt gatgtcctgc gccgcgagcg
    23581 gagtctgttg gcaccaagca agccctccga cgattgcgac gaccttgagc gagtaactca
    23641 gctcctcggt gaagcgaccg ctccgcgctt gcgaaatgct gtggtgggca tcgttggatg
    23701 cagcggcctc ggcactcccg cagggcatgt gctggctagg tcgggaatcg gagagttcgt
    23761 gctggttgat gtgggcttct tcaagaagtc gaaccacgaa cgcaaccacg cgagccgtgc
    23821 ttcagacctc aaccactctc aaatccccaa agtgcagctg ttgaagcggc tgattcatga
    23881 aatccggcct tcagccaagg tgacttgcct tcaagcagac gtgctggatg acggagtgct
    23941 cgatcaactc gtgcgctgtg acttggtgct tggctgcacg gactcctatt atgcccgtgc
    24001 tgcgttgggg gacattgcca cccatttctc ggtgcctgtt ctcgacttgg ctgtgcaaat
    24061 gggaagcaag gatggtgccc tcaccacgca aaccggcgag attgcccgct acatgccagg
    24121 cctgccgtgt ccatggtgcc gaaaccgcgt caatgcagtg cagatccgag cggaaaccgc
    24181 cagtggcgaa gaacgcgcac aagcacgtgc tgctgctcag caggcacagg atcgcggtga
    24241 agacggcgcg ccctactgga tcggtgatca tccgcaggag ctcaccgtcg gctacatgac
    24301 atccatcgtt ggatcattgg gggctggata tgcccacaac tggctcactg gtgctgcaac
    24361 catgccgcac aaccgaatgc agttcgacat tggcaaaacg gcctttggat tcgctgatga
    24421 tgacgagcgc cattccatct ccgactgctc ttgcaacaag tgcgttggct ttgccgacca
    24481 aggacgagcc ttcttcacgg tgtcgcgcag gtagtagcaa cctgaaccag ccgacgctta
    24541 cactcactgg cttgccacga ggcaggacag tagtggcgcg tggggtatta aacctcaccg
    24601 gcttgagaca ggaggcagct tcaaaatccg agaggtgagt tccgcggtga catcagcatt
    24661 ctgcgcggag acatcgtgaa gctcctccgg atcggtttcg tgatcgtaga gttcggtttg
    24721 gccgtcactc cagcgggtga agcgccagcg ggcggtgcgg atgctttggc cgtgcagttt
    24781 ctcaccacgc gtcacgagcg tgaaggccgc gtctttgatg ctggcggtgg gatcggccaa
    24841 aacggggcgc aagctggctc cggcggctgt gtgaggcatt ttcaggccgc agaagtcggc
    24901 gacggtcgga tacacatcaa cgaactcggc gagcgagcgc gtcgtctggc cgcctttcat
    24961 gcccggcgcg gcgatgatga gcggtgcacg gcaactgcgc tcgaacagcg tgtttttgtt
    25021 ccaccactgc cgctcgcccg tgtggtagcc gtgatcgccg acgaagacga cgatggtatt
    25081 ttcccagagt tgatgtttgt cgagcgcatc gagcacgcga ccgagctgcg catccatgaa
    25141 gctcgtgccc gcgcagtagg cgcgaaacag ctcctgccac tcctgcgtcg tgaatttggc
    25201 aaacgcttcg ccgtagtcgc caaagccaac gctctctttt cgcgccgccg tcatgtcagc
    25261 gggatcgctc cagggcttca gcgagcctgg cggatagaga tcgaagtatt tcttcggcgc
    25321 gatgaaggga tcatgcggct tcataaaccc gcagccgatg aaccatggcg tgtcgccgag
    25381 cttctcgatc atcgccaccg tggccgcagc gatctgacca tcgggctgat cgtcatccgt
    25441 gccatccgcc gcgccccact ggcaccattt cagcgccccg ccggtcacgt cgcggccttc
    25501 gagcattttg cggcctgcct tcgtcgcttc aaaagcctgc gccgtgtgcc aggagcggcc
    25561 cgcgtccatc cagcgctgct tcgcttccgc attcttgccg ccgccgagat ggtagagctt
    25621 tccaaacgca tgagactgcc agcctgcgtc tttgcagagc tgcggcagcg tcacgatctc
    25681 cggcatcgtt ttgcgcaaag gaatgtcatt tcccacgatg cccgtctgct ccgcccgcag
    25741 tccggccatc atgctgctgc ggctcggatt gcacaccgga tactgcacat acgcccgctc
    25801 aaacgtcgtg ccgcgagccc gcaaccggtt gaggtgaggc gtcttcacga agtccttcgt
    25861 gaacgccccg ccaaagtccc gcaaatcatc cgccgtgatg agcagcacgt tgggcttcgc
    25921 gaaagcagcg agcagtggac aaaccgagag cagaagcaca aggaggcgca tggacgcgct
    25981 gaaacgacgt cgcgcggttt tttccttcag gccaaatcca aatgcccggt acagttgaag
    26041 gaaggagagt tggtcggtag cggggaagac cccgaagtta cacttggtgc tccattggag
    26101 gtaatgtcgc aagaggatct tcaatgtctg agcggtgggt cttttctcag gcaagctcga
    26161 aggcattcag cggcggtgtc gaggcagcga tgatgcgcac gaaatgcgtg ccgcgtccgg
    26221 ctgccatttt tggctgagcc tctttttgga agatcggacg cgagcggcgg gcgtcaggct
    26281 gaacgcggtg ctggagtgcc cctcctacag gcgggtggcg ctggtggtgc gcccgggcag
    26341 atatgcgggt ttttgccgga gtttgcgagg ccctatctgg gcggaggtga gccggtgatc
    26401 cgcagggcgc tggcctatca gcgcggggtg acgctggcat ggcgggaggc ggcggtgaag
    26461 gcgcggccgt ttttggcggg ggtgttcg
//
LOCUS       DAOMYJ010000050        26470 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Prosthecobacter sp. isolate AMR_MDS_5629
            NODE_671_length_26470_cov_24.046716, whole genome shotgun sequence.
ACCESSION   DAOMYJ010000050 DAOMYJ010000000
VERSION     DAOMYJ010000050.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867850
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prosthecobacter sp. (wastewater metagenome)
  ORGANISM  Prosthecobacter sp.
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Verrucomicrobiaceae; Prosthecobacter.
REFERENCE   1  (bases 1 to 26470)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 26470)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.30
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:34:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,742
            CDSs (total)                      :: 4,684
            Genes (coding)                    :: 4,676
            CDSs (with protein)               :: 4,676
            Genes (RNA)                       :: 58
            tRNAs                             :: 55
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26470
                     /organism="Prosthecobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_671_length_26470_cov_24.046716"
                     /isolate="AMR_MDS_5629"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1965333"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..332)
                     /locus_tag="PLP58_07345"
     CDS             complement(<1..332)
                     /locus_tag="PLP58_07345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009961099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HRJ08365.1"
                     /translation="MNTKMYVGNLPFKASEADLRDLFSQFGGVTDVFLPMDRESGRPR
                     GFAFVSMDTPEAMNAAIAGLNGKDFGGRVLTINEARPKEERPAYGGGGGGGRGGYGGG
                     GGGGRGGYG"
     gene            complement(502..1800)
                     /locus_tag="PLP58_07350"
     CDS             complement(502..1800)
                     /locus_tag="PLP58_07350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009964457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P N-terminal domain-containing
                     protein"
                     /protein_id="HRJ08366.1"
                     /translation="MRYPPLPADLFTSNRQRLKAMLPPRSLVILHANDVMPANADGTL
                     GFVQNSDLYHLSGVDQEETILMLCPDAPDPKQREMIFVRETNEQVALWEGEKLTQEQA
                     AERSGIQRVSWLQDFETAFRQLMCYADQVFLNSNEHPRATSPVETRETRFTRRVRREY
                     PLHDLRRLAPLMHRLRSEKHPAEIAALREAARITGDGFRRVCRFVKPGAHEFEVEAEL
                     AHEFLRQRARGFAYTPIIAAGANACVLHYITNHQECKDGDLLLLDVAANFANYNADLT
                     RTIPVNGRFTPRQRQVYDAVLRVFRAACSMLRPGVILREYQDEVGRLMTTELLGLGLL
                     DRDAVEKQDPDKPLFKKYFPHGTSHHLGVDVHDVGATWEPLRAGMVLTVEPGIYIREE
                     GLGIRLENDILIGETENTDLMADIPIEAEEIEALMSARAV"
     gene            1967..4144
                     /locus_tag="PLP58_07355"
     CDS             1967..4144
                     /locus_tag="PLP58_07355"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HRJ08367.1"
                     /translation="MKLLPPVFTALLGAFASAADRPNIVFFLTDDQTSGSLGCYGHPL
                     AQTPNLDSLAARGTRFQNAFVSHSICWVSRTTMLTGLMGRSHGTKEEPEHLTAENART
                     FYTDLLRQTGYRTGHYGKWHTKLPKGSKPADHFDVFEDIGRNPFFKKLPDGSLRHETD
                     LIVDRGIEFVKQQPKDKPFALNMWFNACHAEDADRRPGLHFAWPESANGLYADQEMYR
                     PRLDDPAIFEALPDFFKTSITRERYFWRWNTSEKYQTNMRAYLRQVTGIDHAIGRFLK
                     ALEEAGLADNTIIVYSADNGYHMANRGLTGKWSHFEESLRVPLIIADPRVEKTQRGKV
                     SEAAALNVDLPSSFLDWAGVPVPETYHGRSLKPLVESARPADWRTETFHEHFAVRNRI
                     PAYEGVRTERFKYVRYFDHDHESLHDLQSDPDELKNLASDPAQADTLKKLRQRTDEFV
                     AQYGGALPPMKGQFQKSTDAHPVASAAVSVPPGPDGWVNLFNGRNLNGWDGDPQYWSV
                     EDGAITGKTDGTLKMNCFLTWKGSTIRNFDLRVKVKVTAGGNSGLQYRGAMLPEVGLD
                     IVSGYQCDIVANNPDYNGMLYEERGRRILSHTGEKVIVAPDGRPWIVGKMEVRQFAPD
                     EWHDYRVLVRGNHHQHWIDGHMTADLMDFDEKGRSLDGVLGVQVHVGPAMKIQFKDFK
                     IQHLPDDLPLLKVEEHPIPAGSPGVKPQGKLPKDWQPPVYGGK"
     gene            4344..7733
                     /locus_tag="PLP58_07360"
     CDS             4344..7733
                     /locus_tag="PLP58_07360"
                     /inference="COORDINATES: protein motif:HMM:TIGR02604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HRJ08368.1"
                     /translation="MKTPACLATLLAVVSAHALTPQEMQQRQQPVVTEMPAMKNQPES
                     FSGLVKHPGLSGSTWVRFPFVQNPGSLGIDRKGRIFVAEVNRFWQGVADLRGINEFIR
                     GDFQSRTLEDRVKLQNSIPGRVPEGFWTNTADRLIRLEDADGNGAADKRSVFADSFYE
                     QLDGLGFSVLPEDDAVYFTCIPSVRKLTDANDDGVADTNDPLVTGFGVRVSFIGHDLH
                     GIVRGPDGRLYFSIGDRGYHVTTKEGKVFEAGGKGAIFRCESDGTGFEVFCNGLRNPT
                     ELAFDEHGNLFTFDNTGDIGDKARMVYALEGTDSGWDMSHQCAHQYVDHMDWEEFHPK
                     VSVWVAEKMFATHGENRPQWAYPPAANVSRGPSGLAWITGQTAPEDLRGKLLLADYGG
                     APQSCKITAYGVKGSGAGYSLASEEVIVEGVGASDVEQGFDGSIYICDFGGGWSVNSN
                     GAIHKLTPKDASLQKAAAELAAMFKRGLTDDLPNQLATALASPDRRLRQMAQFELVKR
                     GEAGQAVLLASAKNAAQPVASRLNGVWGLGQLARQSEAADRAALIELSKDTDAEIRAN
                     AARVLGDAGGEGVQARLLEMTADESPRVKSLAIIALSRVAKTGDAAAIAAIHAAAKAN
                     AGTDIVLRHACGTALKRLGTVESAVALASNASAELRLLAVLQLRHQESAELARFLADR
                     DAMIRDEAIRAIYDTAALDTSAGEALSKLSPTAEMPLLVQRRIVCASYRRGNAQQLLQ
                     HAANAALDISVREAALHGLRLWEKRITTDPVLGGYRPISGGARNLKELGGSIGGALKQ
                     FLASQPPQKLAALALKLADDTGTQLETTTLSTLVSDAKQQAAVRTAALDSLVKSAPGE
                     ARRLVTAHLTDTNAEVAAAALRHGLAMKLDGLAAAARKAADSGPLPAARAGLEIIAAL
                     HPAEALERWTKREANGLRRELWLDLFLALQTSKDATAQQAAATFAASAMDAVPMLSIT
                     GGDPKAGEVVFRNQGACLQCHKVGNDGGIQGPALDLVAERLKPEKIVESLLNPNAEIT
                     AGYGMSSVTLADGALVAGRITEDTPEKLTVIGLDGKAGTYPRSQVKTVTPPVSAMPPM
                     AMALPPPMLRDLVAYLRTRDHTTAPKQDTDAHGDENEKVAK"
     gene            7831..8685
                     /locus_tag="PLP58_07365"
     CDS             7831..8685
                     /locus_tag="PLP58_07365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009963340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HRJ08369.1"
                     /translation="MKRAINTGNGNWQETTLDGPRLRRWMLDASACVELARSRIARIG
                     FDEALPPYRRVRISPGGSFVLACVSGSGFILLDGKWQRVGPGMVCLAPPRVLNALYAA
                     GSERWSFAWVRYDEPAFVAPVVGASSPVVSKARADEASRLVSGLRAEWEGEREPRMIH
                     HWLELIQATVRRFAQPWRSREPRVTKLWTEVGEDLARDWTLEELAHRCHCSSEHLRRL
                     CMREMGRSPMQHLTCLRMEKARQMLESSSDKLEAVAAGVGYANAVIFSRVFKRWVGVA
                     PGEYRGRL"
     gene            complement(8778..10148)
                     /locus_tag="PLP58_07370"
     CDS             complement(8778..10148)
                     /locus_tag="PLP58_07370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ08370.1"
                     /translation="MKRRLFAQGLLAAPLLNLHAAEGGLRIATFEVDVTPPLESPLCH
                     GNVMPVKQIVTPLLARGLVILGSGQPIVLCALDWVALANASHVLIRETIAKAVGTTSD
                     RVAVHTVHQHDAPGSDLDTERLLTEHGLGGRFSNAQLDQRVFEAMADAARACLTKAES
                     VTQVGFGSGKIEKVASNRRVLAPDGKVAFTRMSASKHPQAATAPEGVIDPLLRMVTFW
                     QGERPLAALSYYATHPMSYYGKGGVNWDFIGGARTLMDKAAPGVCFIHFNGAGGNITA
                     GKYNDGSPENRPVLAERVFAGMKAAWESQKKQIITAADIEWRIEPVALPLRNPEAAGA
                     LLKTLADASLPFAKRAFATREAVFIQRAREGVKIPLTCLRLGGARILHMPAELFIEYQ
                     IAAQQTRPDDFIAMAAYGDNAPGYIGTRTSYTQGGYEVGPVSRVSPDVEDVLHSAMRA
                     LLATPA"
     gene            complement(10165..11712)
                     /locus_tag="PLP58_07375"
     CDS             complement(10165..11712)
                     /locus_tag="PLP58_07375"
                     /EC_number="3.1.6.-"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arylsulfatase"
                     /protein_id="HRJ08371.1"
                     /translation="MKSHRTLLLSFFVILAISCGLSFAAPPNIIVIMADDMGFSDLGC
                     YGGEIQTPNLDRLAAEGMRFTQFYNCALCGPSRAALMTGQHPHQVGITQWTGLLNDRC
                     VTMFEVLNRAGYETCAVGRLDMLTAEDWREPENLTRHVGRYFGTTGHQGPGNYFADVR
                     NTAFYRDGKPYSIPEGGYKTDLITDFTLEFLQQRDPAKPFLLYMAHYAPHWPLHAKEA
                     DIAKYRKLYRELGWDTAREQRLKRLIEKGLLPASSQLAARDAKAKPWGEAQNLDWEAE
                     RMAVFAAQIDSLDQSVGRVMEAVRGTNTLVFFLSDNGASDRSVGQLDKPDRTWRSDGT
                     LTRVGNKPGIMPGPADTFVTAGPAWSCLSNTPFRDHKQSNYEGGIASPLIAWWPGVTK
                     AGQITHELSHITDITATVLDVAGQTCPEKFDNRVIAPPAGKSLLPVLEGKPRAGHESL
                     CWATSGHRAVRMGEWKLVSPPAGAWELYNMKQDRAETRNLSNEHPERVAVMAKIFDAW
                     HRLWQPN"
     gene            complement(11747..13036)
                     /locus_tag="PLP58_07380"
     CDS             complement(11747..13036)
                     /locus_tag="PLP58_07380"
                     /inference="COORDINATES: protein motif:HMM:NF024991.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HRJ08372.1"
                     /translation="MTPDKQQAIHRFRTTPDNLLIVGQAGTGKSFLLKHLTEGIPPNK
                     YAIVAPTGMAALNCEGSTIHGMFHLPVGRFPFGIISRDGDWLKNLKRNGEFEGLEILF
                     IDEISMVRSDVLDALDHVLRTQGPNPAAPFGGVRLVALGDPLQLPPVEKINHKPWFDG
                     SKRNAWRSPWFFGAKAWADAAFKTVELTTNHRQESDPEYAKLLDWARLGDLIEMHHEL
                     LDGLKRSLPDQNRALRVMCTNKQVADYNSQQLYKLEGDALRFIATEQRWTGDTPVEKD
                     ILLKVGARVMLRANISVAENWVNGTLAEVVEIDKSNERVVIQNQIGDKKTVARHTWTY
                     ELSCRRANKEPDEATFTQIPLTLAWAITIHKMQGQTATSPIVLEADNAFVGGQVYVGL
                     SRVCSRSQICIPNTLRRIWPERRALNYLKKTLPHRYW"
     gene            complement(13050..14435)
                     /locus_tag="PLP58_07385"
     CDS             complement(13050..14435)
                     /locus_tag="PLP58_07385"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HRJ08373.1"
                     /translation="MRSSLPVLFAILAIFCGQSSATSPNIVFMYADNVGYGDLGCYGN
                     EEVKTPHLDQLAADGALCTDFYVVTSTCTVSRGAVLTGRHPLRNGLLHQLVVEENWHG
                     VGLPHRERIMPQYLQDAGYATACIGKWNIGFAPGSRPTERGFDEFLGCRSGNINYFTH
                     TYHGEYDLFRGTELHRMEGYSGDLFAEASADFIQRQSKAGKPFFLYLPFNAPHYVSKI
                     NVAEGETPQWQAPDRAFAAYGWDPSETDEKRRYLAVITAMDDNVGRVLAALDAAGQRD
                     NTLVVFVSDMGPILRPTHGLGVASAGIYRDGAPTLYEGGIRVPAIFRWPGKIRPGTVT
                     KTMLSHLDLLPMFLNVAGLPSRNDRILDGRDCLPALAGDASSPHERLFFHLRDTVAVR
                     EGNLKLIRPKAKAPWELYDLATDPSEKQDLAVKGPQEVERLANLITNWQLDVKNDASE
                     PVKYKPAKKQN"
     gene            complement(14546..15688)
                     /locus_tag="PLP58_07390"
     CDS             complement(14546..15688)
                     /locus_tag="PLP58_07390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ08374.1"
                     /translation="MKPTLLALTLAFLSTSAFAKVERIWMTHQSPDNSKIVISWETTN
                     PGDSVVEFGTSPQLGESAKVDGSRTLHHVEIPIPQKDVVYHYRVKSGDQVSDINTFKG
                     YPSKLLRIAVVANIRADAKLNFAAIKKDDPHLLISAGDTAMQYQFATQADKEATKSHS
                     TAIDTQADLFKSTPFMVSLGNLDRKIRPNGISMEEPGYDIAATGYRKFFALPGKEWIW
                     AWDIPDFDLRLISVDMSHTGDFGKPNQACNAFDKDSEQFKWFEETMNSTKAGFVITVY
                     GETSGTVRRHAEGGWKRVIEKSSIAISGECYHAERTEVDGMTYYNSSVRGNGTFGHDT
                     KAAFSDKSASYKLITLDRDAGTMTVEIKALDDGRVLDSKTFKKRAQ"
     gene            15772..16173
                     /locus_tag="PLP58_07395"
     CDS             15772..16173
                     /locus_tag="PLP58_07395"
                     /inference="COORDINATES: protein motif:HMM:NF012354.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HRJ08375.1"
                     /translation="MSWPLSTDSYSRFCCGCFRSGWHFKYHHLRYFRAITHEGSLTKA
                     AKHLKLSQSALSVQLRSLEENLGQPLFERKHKALVLTEAGQIALEYADSIFRSGEELT
                     DLLQNRAKRSRGFLRVGAASNLSRNFQLTFL"
     gene            16204..16707
                     /locus_tag="PLP58_07400"
     CDS             16204..16707
                     /locus_tag="PLP58_07400"
                     /inference="COORDINATES: protein motif:HMM:NF015431.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HRJ08376.1"
                     /translation="MIHSGTLRELLTQLQARTLDVVLSNTPVRRDAETGWHSHLLDEQ
                     TVSLVGHKTRGMKPFRFPEDMRKTPVVLPSLESSIRAPFDVLMDQTGIRPIIAAEVDD
                     MAMLRLMACETNGVTLVPPVVVRDELESGALVERHCFPQIKESFYAITPSRRFPNPIL
                     RELMKRK"
     gene            complement(16711..18207)
                     /locus_tag="PLP58_07405"
     CDS             complement(16711..18207)
                     /locus_tag="PLP58_07405"
                     /inference="COORDINATES: protein motif:HMM:NF024627.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HRJ08377.1"
                     /translation="MQPGEVTTPFPTLNHIAIEWQIEGDDDLDATCEVKVRQAGETTW
                     RGAEALRRVPAGKSQKTSPIFTWANRLSGTVFDLEAGTSYEIELKLHDPDGGSATKIV
                     KASTRPEPIAKADAVVKNGSKGDLNAVKPGEVLLLADGDYGAATFNRDGEPGRPIVYR
                     STSGKAVFSEISLTNRKWVYLEGVTVNGPVRLNGSEHCVVRRCSIRSQFGIKAYKPGM
                     MNGCIEDNVITGIREWKPQIMGAGGDNEGEGIQFTGSGNVIRHNRVSGFRDCISHIED
                     DGAAPQMCNDILNNDLSAGLDDGIEADFALHNCRVMRNRLTNCFVGISSQPGLGGPNY
                     FRRNVMFNIILEPFKLHRFSQGDVIQHNTVVKAGDGLGIHTSEPFDHAVITHNLIIGG
                     KPPEGAKYGGYGPGRGRAVDAQSFGDHCLIDHNAYAVLGMPFEGKIRSWTFQKLPGTE
                     FEKHGRELKVPVSLPEDPARLYEPPDFSALTEAGAYSGEKISVYGPRQ"
     gene            complement(18371..18877)
                     /locus_tag="PLP58_07410"
     CDS             complement(18371..18877)
                     /locus_tag="PLP58_07410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ08378.1"
                     /translation="MNPSRQSRPRVQLWMLRLMIAILLTGLPGALLPEIAFQKFAWLM
                     GYGKQPITPLIVYLSGNAGFAYVTIAVLIWAISRDLTRYQPLVRLCGWTMVVAGPAYL
                     LIDLQCPLPLWWTLMDSVGCFLIGITLLWACPEWHGIKTCFRRKWLISSAWQAGMRSS
                     EALGAARL"
     gene            complement(18979..19911)
                     /locus_tag="PLP58_07415"
     CDS             complement(18979..19911)
                     /locus_tag="PLP58_07415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ08379.1"
                     /translation="MKVLLCLSLTSLALAQHPQLKTGPNADLGGVLLMPANDKWNRDV
                     SKDEVDPLSDAIIASIGAETGLHEDFGIVWKGQPGGIPYYVVSGDQPKVPVSFEYADE
                     SDPGPYPIPPNAHIEGGPQSEGDRHVLVLDKDNWKLYELFHSFPLDGGKSWKAGSGAI
                     FDLKNPKPRPAGWTSADAAGLPILPGLARYDEICGAKKLTHALRFTVKKSRRAYVPPA
                     THFASPHKDENLPPMGMRVRLKASFDTTKFTGAAKVILEGLKTHGMILADNGGDWFIS
                     GAPDDRWNHEELLPIRKVKGKDLEVVKMGPMTTR"
     gene            complement(19973..21787)
                     /locus_tag="PLP58_07420"
     CDS             complement(19973..21787)
                     /locus_tag="PLP58_07420"
                     /inference="COORDINATES: protein motif:HMM:NF024120.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 28-related protein"
                     /protein_id="HRJ08380.1"
                     /translation="MKPFLLLLAISSLAAQTPIIFPKDGGVLDVREFGAMAVDDRDDT
                     VAIQKALDAFPNGNRIVYLPAGIWIVTDTLHWPKGEGGNGEKRTILQGAGMALTKIVV
                     PPSTPAFGDQPKAVLWTGVKPAQRFRNAVRDLTIKIGAANPQAIGMQFNASNQGTTRN
                     VTLRAAKDSGLIGLDMGFTDEIGPLLVRNLTVEGFETGIVTKWPVNSNTLEHITLRGQ
                     RKLGWHNYHQMIFVRGLTSENAVTTLYNEKDSWGTVTLVDSKITGTGIDAPGLLNQRQ
                     MYLRDVEITGHVVSIDHADKGRDKGDVKEAGRITEDTSHANVAAPFRFEGLKTFAEAG
                     KVTHLPVKETPEVPWDGKWVNLEAFGADPEGKQDSSAALQRAIDSGAETIYLPAGKEF
                     FFDSEVIIRGAVRRIIGLEGRFHTTGKAVWRLVDGSNDAPAVIIERMSNRSGSHNIEL
                     RHESKRTLIVSSAIGFRVEGHGSSDIFVDDLCGQLNLQKPGQSAWCRQLNVEHEGTMC
                     RNHGGNLWILGMKTAKIGTIIQTLNGGITDVSGVFIYANRGWEPDLPAFICHDSTLTL
                     SGINDRNYSQRPIVIWTRETQKGETRDLKERTWVYLSR"
     gene            complement(21846..22754)
                     /locus_tag="PLP58_07425"
     CDS             complement(21846..22754)
                     /locus_tag="PLP58_07425"
                     /inference="COORDINATES: protein motif:HMM:NF012958.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase-fold protein"
                     /protein_id="HRJ08381.1"
                     /translation="MKLILLLFFALFVSIAGAQGPSWVTPAVQAPHVAHHTFESSALQ
                     SKVSFHAYTPEVYELEKERRFPVLYWLHGTGGGLAGIAPVSAWFDAAIREGKMPPVVV
                     VFPNGLATSMWCDSKDGAVPMETLVVKELVPHIDATFRTFAKREGRIVEGFSMGGYGA
                     ARLGLKFPEVFGAASCLAGGPLDLDFQGPRTRANPAERECILRTTFGGDLDYFRAQSP
                     LTLAEKQAAAVRGKVFMRVAAGSRDFTAALNREFSSHLKRLNIDHTFIGVPGVGHETL
                     PLLRGMGNEQWQFYRAALAAVSPTIR"
     gene            complement(22751..23692)
                     /locus_tag="PLP58_07430"
     CDS             complement(22751..23692)
                     /locus_tag="PLP58_07430"
                     /inference="COORDINATES: protein motif:HMM:NF014305.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHB depolymerase family esterase"
                     /protein_id="HRJ08382.1"
                     /translation="MSIEPTAMAEPLAAGDHTRTVKVGELNRRYQIHVPGRYDSTKAT
                     PVVIAFHGGGGNPASMVRLSGLNAKSEEAGFIVVYPFGTGRLENQLLTFNGGGCCGYA
                     MENKIDDVAFTAALLDDLAMVVKVDANRVYATGLSNGGIVSHYVASELSDRIAAIAPV
                     GGPLMMDAVNAKRAVPVMHFHGTADAFAPFQGGYGKGSFGQRKGVTDFRSVEHTIQTW
                     VKNNGCNATPEVVPLPDKAADGMKCTRKTWGGGKDGSEVVLIEIEGGGHTWPSMKPIV
                     EMLGPSTMDISANHLMWDFFQKHPLKPATAAKGTSKP"
     gene            23886..25268
                     /locus_tag="PLP58_07435"
     CDS             23886..25268
                     /locus_tag="PLP58_07435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ08383.1"
                     /translation="MHLLRSILLIILVSTAVQALTPDEAEARLRMARQIARERNDREL
                     VQQVEKLAREFKAGLPAEAEEQLREAEKAVGIDPGGWSMAGQPLFHPTAAMEAALKAE
                     GPKLAAAMASGDANAVREITRVMEGILGDQAGVPDGRRMGQKPSELKLSRAEVVKLFL
                     AALETEGRAMRTLMKGELLPDQMVRVYAYVLDACVTMHPHVAQHVPERVADVEKLLRG
                     TASVLLRLQQPQGHFPFPDLRGKNIRFGDMTTKHLQNGTIEIQDGWIITPDPDGGSQF
                     DTGVCGVALLRAGELLKEEGYLAAGRRAAEWAAKQKCCANFNYNAFSVSLLARAGMKE
                     AALEKFRVGVAPGQAKNGRWLDAHNVRTVYHVIILRALADLGRSAEVDAVALPAIHAL
                     LDEFDAMGITVEALPELHALAKQHPDDARLQKAFRDMASTLVNKCTDGTRVKMGAQPH
                     QLAAVVEVVE"
     gene            complement(25289..>26470)
                     /locus_tag="PLP58_07440"
     CDS             complement(25289..>26470)
                     /locus_tag="PLP58_07440"
                     /inference="COORDINATES: protein motif:HMM:NF019222.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1552 domain-containing protein"
                     /protein_id="HRJ08384.1"
                     /translation="SASPTPRRMICICAPLGFYPEHFFPKQAGKDYALSTYLDSLKDH
                     RDDFTVISGLQHAGMGSSFAHQASASFLTGAPGAGRPGFRNAVSLDQFAAEHLGAATR
                     FPSLVLSGEGSGGLSWTRTGALIPADNSPSKVFARLFLEGEAAEVAQQMRQLEDGRSI
                     LDDVRSQAKSLLSGLGSEDRDKLDEYLTSVRELEQRMVNDESWARKPKPKIDVKPPQD
                     IQNAADLIGRTRLLFDLSHLALQTDSTRLITIMLAGNTQVPPIEGVSLGHHDLSHHGK
                     DPSKLSQLQIVEQETMKTVRDLLSKLQQSKEADSNLLDHTTVFLGSNLGDGSSHSTKN
                     LPVLLAGGGFNHGQHLAFDPDNPPPLCNLFVNMLQRMGIETDKFGTSTGTLTGVEMKP
                     V"
BASE COUNT         4950 a         7980 c         8299 g         5241 t
ORIGIN      
        1 ccatagccgc cgcgaccacc gccgccgccg ccgccgtaac cgccgcgacc accaccgccg
       61 ccgcctccat aagcggggcg ctcctctttc gggcgggctt cgttgatggt cagcacgcgg
      121 ccgccaaagt ctttgccatt gagacccgcg atggcggcat tcatggcttc gggagtgtcc
      181 atggagacga aggcgaagcc gcgcgggcgg ccgctctcac gatccatcgg gaggaagacg
      241 tcggtgacgc ctccaaattg ggaaaacaga tcgcgcagat ccgcttcgga tgccttgaag
      301 gggagattcc ccacatacat tttggtgttc attgtcggtg atgctgaatg tgtctctgat
      361 cgtgacctgc cagaaacctc accgacgaaa cgctcacctg atgaatcttc tgacctgacc
      421 ttcaaacagg tggacggcga aagctcaacg agaagacgtg ctttgcaagc gggattccta
      481 ttttttcctc tgacgtggcc gtcacacggc gcgcgcggac atcagcgcct cgatctcctc
      541 cgcctcaatg gggatgtccg ccatgaggtc ggtgttttcg gtctcgccga tgaggatgtc
      601 gttttccagg cgaatgccca gcccttcctc gcggatgtaa atgccgggtt cgacggtgag
      661 gaccatgccg gcgcgcaggg gctcccaggt ggcgccgaca tcatgcacat ccacacccag
      721 atggtggctg gtgccgtgcg gaaagtattt tttaaagagc ggcttgtcgg gatcctgctt
      781 ctccacggca tctcggtcca gcaagccgag gcccagtagc tcagtggtca tgagacggcc
      841 cacctcatcc tggtactccc gcaggatgac acccggccgg agcatggagc aggcggcgcg
      901 gaagacgcgc agcactgcat catagacctg gcgctggcgc ggggtgaagc ggccgttgac
      961 cggtatcgtg cgtgtgaggt cggcgttata gttggcaaag ttcgcggcca cgtcgagcag
     1021 caagaggtcg ccgtctttgc attcctggtg gttcgtgatg taatggagca cgcaggcgtt
     1081 cgctccggcg gcgatgatgg gcgtgtaggc aaagccgcgc gcccgctggc ggaggaactc
     1141 gtgggccagc tcggcctcga cttcaaactc atgcgcgccg ggtttcacaa accggcacac
     1201 gcgcctgaag ccgtcgcccg tgatgcgcgc ggcctcgcgc aaggccgcta tctcggcggg
     1261 gtgcttctca ctgcgcaggc ggtgcatgag cggggccagg cggcgcaggt cgtgcagcgg
     1321 atactcccgg cggacgcggc gtgtgaagcg ggtttcgcgc gtctccaccg ggctggtggc
     1381 gcgcgggtgc tcgttggagt ttaggaacac ctggtccgcg tagcacatga gctggcggaa
     1441 ggcggtctcg aagtcctgaa gccagctcac gcgctggatg cccgagcgct ccgcggcctg
     1501 ctcctgcgtg agtttttcac cctcccaaag ggcgacctgc tcgttggtct cgcgcacgaa
     1561 gatcatctcc cgctgcttgg ggtcgggcgc gtccgggcag agcatgagga tggtttcctc
     1621 ctggtccacg ccgctgagat gatacaggtc gctgttttgg acaaagccga gcgtgccgtc
     1681 agcgttcgcg ggcatcacat cgttggcgtg caggatcacc agcgaacgcg gaggcagcat
     1741 ggccttgaga cgctggcggt tgcttgtgaa gaggtcggct ggcaggggcg ggtaacgcat
     1801 ggcggcggag tctggacggc gcgcgcgcaa agcacaagcc cgtactccgc gccagccatt
     1861 cctcatgaat ggtgccggat gagaagccgc gcagccactg ccgaatgcga cacactcttt
     1921 gccagcagga tcatcccgca tatggcgttt caatcggaga ccaatcatga agctccttcc
     1981 gcccgttttc actgccctgc tcggcgcttt cgcgtccgcg gcggaccgcc ccaacatcgt
     2041 cttcttcctc accgatgacc agacctccgg ctcgctgggc tgctacggcc atcccctggc
     2101 gcagacgccg aatctcgact ccctcgcggc gcgcggcacg cgttttcaga acgccttcgt
     2161 cagccactcc atctgctggg tcagccgcac caccatgctc accggcctca tgggccgcag
     2221 ccacggcacg aaggaggagc cggagcacct cacagcggaa aatgcgcgca cgttttacac
     2281 cgatctgctg cgccagaccg gctaccgcac cggtcactac ggcaaatggc acaccaagct
     2341 gcccaaaggc agcaagccgg cggatcactt cgatgtgttc gaggatattg gccgcaatcc
     2401 cttcttcaaa aagctgccgg atggctcgct gcggcacgag acggacctca tcgtggatcg
     2461 cggcattgag ttcgtgaagc agcagccgaa ggataagccc ttcgcgctta acatgtggtt
     2521 caacgcctgc cacgccgagg acgccgaccg ccgccccggc ctgcacttcg cctggccaga
     2581 gtccgcgaac ggattgtatg ccgaccagga gatgtaccgt ccgcgtctcg atgatccggc
     2641 gatctttgag gcgctgccgg attttttcaa aaccagcatc acccgcgagc gctacttctg
     2701 gcgctggaac acgtcggaga agtatcagac caacatgcgc gcctacctgc gccaggtcac
     2761 cggcattgac cacgccatcg gccgcttcct caaggcgctc gaagaggccg gactcgcgga
     2821 caacaccatc atcgtctatt ccgccgacaa cggctaccac atggccaacc gcggcctcac
     2881 cggcaaatgg tcgcactttg aggaatccct gcgcgtgccg ctcatcatcg ccgacccgcg
     2941 tgtggagaag acacagcgcg gcaaggtcag cgaggccgcc gcgctgaatg tggacctgcc
     3001 ctcttccttc ctcgattggg cgggcgtgcc cgtgcccgag acgtaccacg gccgcagcct
     3061 gaaaccgctg gtcgaaagcg ccaggcccgc cgactggcgc acggagacct ttcacgagca
     3121 cttcgccgtg cggaaccgca tcccagctta cgaaggcgtg cgcacggagc gcttcaagta
     3181 tgtgcgctac tttgaccacg atcacgaatc cctgcacgac ctgcaaagcg atcccgacga
     3241 gctgaaaaac ctcgccagcg atcccgcgca agccgacacg ctgaaaaagc tgcgtcagcg
     3301 caccgatgaa ttcgtcgctc aatacggtgg cgcgttgcct ccgatgaagg gccagtttca
     3361 aaagtccacc gacgcgcatc ccgtggccag cgcggccgtt tccgtgccgc ccggcccgga
     3421 tggctgggtg aatctcttca acggcagaaa cctcaacggc tgggacggtg atccgcagta
     3481 ctggtccgtc gaagacggcg ccatcaccgg caaaaccgac ggcacgctga agatgaactg
     3541 tttcctcacc tggaaaggct ccaccatccg caatttcgac ctgcgcgtga aggtgaaggt
     3601 caccgccggc ggcaacagcg gcctgcaata tcgaggcgcc atgctgcccg aagtcgggct
     3661 cgatatcgtc agcggctacc agtgcgacat cgtggcgaac aacccggact acaacggcat
     3721 gctctacgag gagcgcggcc gccgcatcct ctcgcacacc ggggagaagg tcatcgtcgc
     3781 gccggatggc aggccgtgga tcgttgggaa aatggaggtc aggcagttcg cgccggatga
     3841 atggcacgac taccgcgtgc tcgtgcgcgg caaccatcac cagcactgga ttgacggcca
     3901 catgaccgcc gacctcatgg acttcgacga aaaaggccgc tcgctcgacg gtgtgctcgg
     3961 cgtgcaggtg catgtcggcc ccgcgatgaa gatccagttc aaggatttca aaatccagca
     4021 cctgcccgat gacctgccgc tgctgaaagt tgaggagcat cccattcccg ccggctcacc
     4081 cggtgtgaaa ccgcagggca agctgcccaa agactggcag ccgcccgtgt atggcgggaa
     4141 gtgatgcgga tctcaagtct cccgcctcac gaacagatgc gctttcaagc ccgcatctgg
     4201 catgctttcc aagctcgaaa taccgcaata ggtttggcag gtttccgctg tcgttgtgaa
     4261 atgactgcag tgcctgtcaa attcggctgg ctggaatcat ggccacagcc gtataatggc
     4321 ttttgcatag cttccttcaa cgcatgaaaa cgcccgcctg ccttgccact ctccttgccg
     4381 ttgtatccgc gcatgcactt acgccgcagg agatgcagca gcggcagcag ccggtcgtga
     4441 ctgagatgcc ggcgatgaaa aaccagccgg agtccttttc cggcttggtg aaacaccctg
     4501 gactcagtgg cagcacctgg gtgcgctttc cctttgtgca gaatcccggc agcctcggca
     4561 ttgaccgcaa gggacgcatc ttcgtcgcgg aggtgaaccg cttctggcag ggcgtggcgg
     4621 acttgcgcgg catcaatgaa ttcatccgcg gtgactttca gtcccggacg ctggaggacc
     4681 gagtgaagct gcaaaactcc atccccggcc gtgtgccgga gggtttttgg accaacaccg
     4741 ccgaccgcct catccgcctg gaggatgccg atggcaacgg cgcggcggac aagcgctcgg
     4801 tctttgcgga cagcttttac gagcagctcg acggcctcgg cttctccgtg ctgccggagg
     4861 atgacgccgt ctatttcacc tgcatcccga gtgtgcggaa gctcaccgac gcgaacgatg
     4921 atggcgtcgc cgacacgaat gatccgctgg tcaccggttt cggcgtgcgc gtttccttta
     4981 tcggccacga tctgcacggc atcgtgcgcg ggccggacgg gcggctgtat ttcagcatcg
     5041 gcgaccgtgg ctatcatgtg acgacgaagg agggcaaagt tttcgaggct ggaggcaagg
     5101 gggcgatctt ccgctgcgag tcggatggca cgggcttcga ggtcttctgc aatggcctgc
     5161 gcaatcccac cgagctggcc ttcgacgagc acggcaatct tttcaccttc gacaacaccg
     5221 gcgacatcgg ggacaaagcg cgcatggtct atgcgctcga aggcacggac tccggctggg
     5281 acatgagcca ccagtgcgcg catcaatatg tggatcacat ggactgggag gagtttcatc
     5341 ccaaggtcag cgtgtgggtg gcggagaaga tgtttgccac gcatggcgaa aaccgtccgc
     5401 agtgggccta cccgcccgcc gcgaatgtga gccgcggacc ttccggcctc gcctggatca
     5461 cagggcagac cgcgccggag gatctgcgtg ggaagctgct gctcgccgac tacggcggtg
     5521 cgccgcagag ctgcaagatc accgcttatg gcgtgaaggg cagcggtgcg ggctattcac
     5581 tcgcgagcga ggaagtcatc gtcgaaggcg tgggtgccag cgatgtcgag cagggctttg
     5641 acggcagcat ctacatctgc gacttcggcg gcggctggtc cgtgaactcc aatggcgcga
     5701 tccataagct cacacccaag gatgcgtctc tgcaaaaagc ggccgcagaa ctcgccgcga
     5761 tgttcaagcg cggtctaact gacgatttgc cgaaccaact cgccactgcg ctggcatcgc
     5821 ccgaccgccg tttgcgtcag atggcgcagt tcgagctcgt gaagcgtggt gaagctgggc
     5881 aggcggtgct gctcgcctcg gctaaaaatg ccgcccagcc agtcgcgagc cgtttgaatg
     5941 gtgtctgggg tcttggacag cttgcacgac agagcgaagc agcagatcgg gccgcgctca
     6001 tcgagctttc caaagacaca gatgccgaaa tccgcgccaa tgccgcccgt gtgctcggcg
     6061 atgctggtgg cgaaggcgtg caggcccgtc tgctcgaaat gactgccgat gaatcgccac
     6121 gcgtgaagtc gctcgccatc atcgcactga gccgtgtcgc caaaacaggt gatgctgccg
     6181 ccatcgcggc cattcatgca gcggcgaaag ccaacgcagg cactgacatc gtgctgcgtc
     6241 atgcctgcgg caccgcgttg aagcgcctcg gcacggtgga aagcgccgtc gcgctcgcca
     6301 gcaatgccag cgcggagttg cgcctgctcg ccgtgctgca gcttcgccat caggagagcg
     6361 cggagctggc ccgcttcctc gctgatcgcg acgccatgat ccgcgatgag gccatccgcg
     6421 ccatctacga caccgccgcg ctcgacacgt ctgctggcga ggctttgtca aaactgtcgc
     6481 ccaccgccga aatgcccctg ctcgtgcagc gccgcatcgt ttgcgccagc tatcgtcgtg
     6541 ggaacgcgca gcagcttttg cagcatgccg ccaatgccgc gctcgacatc agcgtgcgcg
     6601 aggccgcgct gcatggactg cgcctttggg aaaaacgcat caccaccgat cccgtgctcg
     6661 gcggttaccg cccgatctct ggcggggcga gaaacctcaa ggaactcggc ggcagcatcg
     6721 gcggcgcctt gaagcagttc ctcgccagcc agccgccgca gaagcttgcc gcgctcgctt
     6781 tgaagctcgc cgatgacacc ggcacccagc ttgaaacaac cacgctctcc actctggtta
     6841 gtgatgcaaa gcagcaggcg gctgttcgca ccgccgcgct cgacagcctc gtcaaatcag
     6901 cgcccggcga agcccgcagg ctcgtcaccg cgcatctgac ggacacgaac gccgaggtcg
     6961 ccgccgccgc gctgcgccac ggcctcgcga tgaagctcga cggcctcgcc gcagccgccc
     7021 gcaaggccgc cgacagcggt cctctgcctg ctgcgcgtgc aggattggaa attatcgccg
     7081 ccctgcatcc cgccgaggca ctggagcgct ggacgaagcg cgaggccaac ggcctgcgac
     7141 gcgagctgtg gctcgatctc ttcctcgcac tgcaaacctc caaggacgcc accgcgcagc
     7201 aggctgcggc cacctttgcc gccagcgcca tggatgccgt gcccatgctc agcatcaccg
     7261 gcggcgatcc gaaggctggt gaggtcgtct ttcgcaatca aggcgcctgc ctccaatgcc
     7321 acaaggtcgg caacgacggc ggcatccaag ggcctgcgct cgatctcgtg gccgagcgct
     7381 tgaagcccga gaaaatcgtc gagtccctcc tcaatccaaa tgccgagatc accgccggtt
     7441 acggcatgtc ctcggtgacg ctcgcggatg gcgcgctcgt ggcaggccgc atcaccgaag
     7501 acacgcctga gaagctcacc gtcatcggcc tggacggcaa ggcgggcact tacccccgca
     7561 gccaggtcaa gaccgtcacc ccgcccgtgt ccgccatgcc gccgatggcc atggccctgc
     7621 caccgccgat gctccgcgac ctcgtcgcct acctccgcac ccgcgaccac accaccgctc
     7681 cgaagcaaga caccgacgcc catggtgatg agaatgagaa ggtggctaag tgagaaccag
     7741 gcaaaaccgc aggaactccg gcttcccaat ggatcatctg cgcatgagct tcttgcctcg
     7801 ttctccgagt cgctcaacag tgtgggagca atgaagcgcg ccatcaacac agggaacggg
     7861 aattggcagg agaccaccct cgacggcccc cgcctgaggc ggtggatgct ggatgcctcc
     7921 gcttgcgtgg agcttgcccg cagtcgaatc gcacggatcg ggttcgacga ggcgctgcct
     7981 ccctatcgtc gtgtcagaat cagccccggt ggcagctttg tgctggcctg tgtgagcggg
     8041 agtggattca tccttttgga cggcaagtgg caacgggtgg ggccaggaat ggtttgcctg
     8101 gccccaccga gggtgcttaa cgctctttac gctgcgggaa gcgagcgctg gtccttcgca
     8161 tgggtgcgct atgacgagcc tgcctttgtg gcgcctgtcg ttggggcatc ctcgccggtg
     8221 gtgtccaaag cccgcgcgga tgaggcttcc cgtcttgtgt ccggtctccg tgccgaatgg
     8281 gagggggaaa gggagccgcg catgatccat cattggctgg agctgatcca ggcgactgtc
     8341 cgccgcttcg cgcagccgtg gcgaagccgc gagccgcgcg tgacgaagtt gtggaccgag
     8401 gtgggggagg atttggcgcg cgactggact ctggaggaac tcgctcatcg ctgccattgc
     8461 agctcggagc atctgaggcg gctatgcatg agggaaatgg gccgcagccc gatgcagcac
     8521 ctgacctgtc tgcgcatgga aaaagcgcgt caaatgctcg aatccagcag cgacaagctg
     8581 gaggccgtgg cggcaggcgt gggttatgcg aacgcggtta ttttctcgcg ggttttcaaa
     8641 agatgggtgg gagtggctcc gggcgaatat cgcgggcggt tgtgatccat ttgatggggt
     8701 ttaggcatcg ctgaaaagca aggcatccat tcttgaggca ttgccgtcct tgtctgttcc
     8761 gcgcaccggc tcagatttca tgccggggtt gcaagcaacg ctctcatggc ggagtgcagc
     8821 acatcctcca catcaggact cacgcgcgac actgggccga cctcgtagcc gccctgggtg
     8881 taggaagtgc gcgtgccgat gtagccgggg gcgttgtcgc cataggcggc catggcgatg
     8941 aaatcatcgg ggcgcgtctg ctgcgcggcg atctggtatt cgatgaacag ctcggcgggc
     9001 atgtgcagga tgcgcgcgcc gccgaggcgc aggcaggtca atgggatttt gacgccttca
     9061 cgggcgcgct gaatgaacac cgcctcacgc gtggcaaagg cccgcttggc aaacgggagc
     9121 gatgcgtccg cgagggtttt gagcaatgcg ccggcggcct ccgggttgcg caacggcagc
     9181 gccaccggtt cgatgcgcca ttcgatgtcg gcagcggtga tgatttgctt cttttgcgac
     9241 tcccacgcgg ccttcatgcc cgcaaagaca cgctcggcga gcacggggcg gttttccggg
     9301 ctgccatcgt tgtatttgcc tgcggtgatg ttgccgccag cgccgttgaa atgaatgaag
     9361 cacacgccgg gcgcggcctt gtccatcaaa gtacgggctc cgccgatgaa gtcccagttc
     9421 acgccgcctt tgccatagta gctcatcgga tgcgtggcgt agtagctgag cgcggccagc
     9481 ggacgctcgc cctgccagaa ggtcaccatg cgcagcagcg gatcaatcac gccctccggc
     9541 gcggtggcgg cctgcggatg cttcgaggcg ctcatgcggg tgaaggccac cttgccgtcc
     9601 ggggcaagca cgcggcggtt ggaggccact ttctcgatct tccccgaacc aaagccgacc
     9661 tgcgtgacgc tctccgcctt ggtcaggcaa gcccgcgccg catcggccat cgcctcgaac
     9721 acacgctggt cgagctgcgc gttggaaaat cgcccgccaa gcccgtgctc ggtgaggagt
     9781 ctctccgtat ccaaatcgct gcccggcgcg tcatgctgat gcaccgtatg caccgccacg
     9841 cgatcactcg tcgtgccgac ggccttggcg atggtctcgc gaatcaggac atggctcgca
     9901 ttggccagag ccacccagtc cagcgcgcac agcacgatgg gctgcccgga gccgagaatg
     9961 accaaaccgc gcgccagcag cggcgtgacg atctgcttca ccggcatcac attgccatgg
    10021 cacagcggcg actccagcgg cggcgtgaca tccacctcaa acgtggcgat gcgcaaaccg
    10081 ccttccgcag catggagatt cagcaaaggc gcggcaagga gaccttgggc gaagaggcgg
    10141 cgtttcatgg cgggtgttag ttcgtcagtt aggctgccag aggcggtgcc atgcatcgaa
    10201 gatctttgcc atcacggcga cgcgttcggg atgttcgttg gagagattgc gggtttcggc
    10261 gcggtcctgt ttcatgttgt agagctccca ggcgccagca ggcggggaga caagcttcca
    10321 ctcgcccatg cgcacggccc tgtggccgct ggtggcccag cagaggcttt catggcctgc
    10381 tcgcggcttt ccttcgagca ccgggagcaa cgatttgcct gccggcgggg cgatgacgcg
    10441 gttgtcgaac ttttccgggc aagtctgccc ggcgacatcc agcacggtgg cggtgatgtc
    10501 ggtgatgtgg ctcagctcat gggtgatctg cccggctttc gtcacgccgg gccaccaggc
    10561 gatgagcggc gaggcgatgc cgccttcgta gttcgactgc ttgtgatccc ggaacggcgt
    10621 gtttgacagg caggaccagg ccgggccggc ggtgacaaag gtgtctgccg gacccggcat
    10681 gatgccgggc ttgttgccca ctcgcgtgag ggtgccatcg ctgcgccagg tgcggtccgg
    10741 cttgtcgagc tggcccacgg accggtcgga ggcgccgttg tcgctgagga aaaagacgag
    10801 cgtgttcgtg ccgcgcaccg cctccatcac acggcccacg ctttgatcca ggctgtcaat
    10861 ctgcgcggcg aagaccgcca tgcgctcggc ctcccagtcg aggttctgcg cctcgcccca
    10921 gggcttggct ttggcatcgc gcgcggcgag ctgtgagctg gctggcagca ggcctttttc
    10981 gatgagccgc ttcagccgct gctcgcgcgc ggtgtcccag ccgagttcgc ggtagagctt
    11041 ccgatacttc gcgatgtccg cctccttcgc gtgcagcggc cagtgcggcg cgtagtgggc
    11101 catgtagagg aggaagggct tcgctggatc acgctgctgg aggaactcga gggtgaaatc
    11161 ggtgatgagg tcggtcttgt agccgccctc gggaatggaa tacggcttgc cgtcgcgata
    11221 aaaggcggtg ttgcgcacat cggcgaagta attgcccggc ccctggtggc cggtggtgcc
    11281 gaagtagcgc cccacgtgcc gcgtgaggtt ttccggctcg cgccagtctt ccgccgtgag
    11341 catgtcgagc cggccgacgg cgcaggtctc gtagcccgcg cggttcagca cctcgaacat
    11401 cgtcacgcag cggtcattga gaaggccggt ccactgggtg atgccgacct gatgcggatg
    11461 ctgcccggtc atcaatgcgg cgcgcgaggg accgcagagc gcgcagttgt aaaactgcgt
    11521 gaacctcatg ccttcggcgg caagccggtc aaggttcggc gtctgaatct ctccgccata
    11581 gcagcccagg tcggagaagc ccatgtcatc cgccatgatg acgatgatgt ttggtggagc
    11641 cgcgaaagac aggccgcaag agatcgcaag gatcacaaaa aaagagagca agagcgttcg
    11701 gtgggacttc atgtgggaag ttggaatgtt cgccttggac gactcttcac caataacgat
    11761 ggggaagagt tttcttcaga taattcagcg cccttctttc aggccagatg cgccttaggg
    11821 tattgggaat gcagatttgg ctgcgcgaac agactcgcga aaggcccaca taaacctgcc
    11881 cgccaacaaa agcattgtca gcttcgagaa caattggcga cgtggcggtc tgaccttgca
    11941 tcttgtggat cgtaatcgcc caggccagtg tcagtgggat ctgagtgaat gtcgcttcat
    12001 caggctcttt atttgctctc ctacagctga gttcataagt ccaggtgtgg cgcgccactg
    12061 tcttcttatc gccgatttga ttttgaatta ctacacgctc attgcttttg tctatttcaa
    12121 caacctcagc aagtgtgccg tttacccagt tctccgcaac ggatatatta gcccttagca
    12181 taacgcgagc acccaccttc aggagtatgt ccttttcgac gggagtgtcc ccagtccaac
    12241 gttgttcggt ggcgataaac cgcagagcat ctccttccag tttgtacaac tgttgggagt
    12301 tgtagtcagc aacctgcttg tttgtgcaca taacgcggag ggcacgattc tggtctggca
    12361 acgatcgctt cagcccatca agcagttcgt gatgcatttc gatcagatca cccagcctag
    12421 cccagtcaag caacttggca tactcaggat cgctttcctg gcggtgattt gtagttagtt
    12481 cgactgtttt gaatgcagca tcagcccaag ctttggcacc aaagaaccag ggtgacctcc
    12541 atgcatttcg tttactgcca tcgaaccaag gcttgtgatt tatcttctca accggtggaa
    12601 gctgaagtgg atcaccaagc gccaccaacc tcactccacc aaacggcgcg gcaggattag
    12661 gcccttgtgt acgaagcaca tgatccaatg catcaagaac atctgagcgc accatggata
    12721 tttcgtcgat aaacaaaatt tcaagcccct cgaactcccc attacgcttg aggtttttca
    12781 accaatctcc atcacgggaa atgataccaa atggaaaccg accgacagga agatggaaca
    12841 ttccgtgaat ggtgcttcct tcacaattca aggcagccat gcccgtaggg gctactattg
    12901 cgtatttatt tggtggaatc ccttcggtca aatgctttag caaaaaggac tttcccgttc
    12961 cagcttgccc tacaatcaac agattgtctg gtgtcgttct gaaacggtga atcgcctgct
    13021 gcttgtcggg agtcatttgt aaatgtcgac taattctgct tttttgccgg cttgtatttc
    13081 accggctcgc tcgcgtcgtt tttcacatca agctgccagt tggtgatcag gtttgcgagg
    13141 cgttcgactt cctgcggacc tttgacggca aggtcctgct tctcggaggg atcggtggcg
    13201 agatcgtaga gctcccaggg tgccttggct ttcgggcgga tgagtttgag gttgccttcg
    13261 cgcacggcga cggtgtcgcg caggtggaaa aagaggcgct cgtggggtga ggaggcgtct
    13321 ccagccagcg cagggaggca gtcgcgtccg tcgaggatgc ggtcatttcg cgaaggcaat
    13381 ccggcaacgt tcaagaacat cggcagaagg tccagatggc tgagcatggt cttcgtgacg
    13441 gtgccgggtc tgatcttgcc gggccagcgg aagatcgccg gcacgcgaat gccgccttca
    13501 tacaaggtgg gcgcgccatc gcgatagatg ccggcgctgg cgacgccgag gccgtgcgtg
    13561 ggccgcagga tggggcccat gtcggagaca aagacgacaa gcgtgttgtc gcgctggccg
    13621 gcggcatcga gtgcggcgag cacgcggccg acgttgtcat ccatagcggt gatgaccgcg
    13681 aggtagcggc gtttttcatc cgtctcggac ggatcccagc cataggccgc aaacgcgcgg
    13741 tccggggcct gccattgcgg cgtctcgccc tcggcgacgt tgatcttgct gacgtagtgc
    13801 ggggcgttga agggcaggta gaggaagaag ggcttcccgg ccttgctttg acgctggatg
    13861 aaatcggcgg aagcctcggc aaagaggtcg ccactgtagc cttccatgcg atgcagttcg
    13921 gtgccgcgga acaggtcgta ttcgccgtgg taggtgtggg tgaagtagtt gatgttgccg
    13981 ctgcggcagc cgaggaactc gtcaaagccg cgctccgtgg ggcggctgcc tggggcaaag
    14041 ccgatgttcc acttgccgat gcaggcggtg gcatagcccg cgtcctgcaa atactgcggc
    14101 atgatgcgct cgcggtgcgg caggccgacg ccatgccagt tctcctcgac cacgagctga
    14161 tgcagcaggc cgttgcgcag cggatgccgc cccgtcagaa ccgcgccgcg cgaaacggtg
    14221 caggtggagg tgacgacgta aaaatccgtg cagagagcgc cgtccgccgc aagctggtcg
    14281 aggtgcggcg ttttgacctc ctcgttgcca tagcagccga gatcgccgta accgacattg
    14341 tcggcataca tgaagacgat gttcggcgat gttgctgatg actggccgca aaagatcgca
    14401 aggatcgcaa agagaactgg aagggagctt ctcatgaccg gctaatgatt ggcaatgagg
    14461 taaaccgatg caggcgccgc tgtattgcat ccaggagcgc ttgattgccg gagagaaatc
    14521 ggtgcccggc cccatccagc aatgctcact gcgcgcgctt cttgaaagtc ttgctgtcga
    14581 gcacgcggcc gtcgtccagg gccttgatct cgacggtcat cgttccggcg tcgcggtcga
    14641 gggtgatgag cttgtagctg gcagacttgt cggagaaggc ggccttggtg tcgtggccga
    14701 aggtgccgtt gccgcgcacg gagctgttgt agtaggtcat gccgtccacc tcggtgcgct
    14761 cggcgtggta gcactcgccg gagatggcga tgctgctttt ttcaatgacg cgcttccagc
    14821 cgccttcggc atggcggcgc accgtgccgc tggtctcgcc atagacggtg atcacaaagc
    14881 ccgccttcgt gctgttcatc gtctcctcga accatttgaa ctgctcggag tccttgtcga
    14941 aggcgttgca ggcctggttg ggtttgccga agtcgcccgt gtggctcatg tccacgctga
    15001 tgaggcggag atcaaagtct gggatgtccc aggcccagat ccactccttg ccgggcagcg
    15061 cgaagaactt tcggtaaccc gtggcggcga tgtcgtagcc cggctcctcc atgctgatgc
    15121 cgttggggcg gatcttgcgg tcgagattgc cgagcgagac catgaagggc gtgctcttga
    15181 aaaggtccgc ctgggtgtcg atggccgtgc tgtgcgattt cgtggcctcc ttgtccgcct
    15241 gcgtggcaaa ttgatactgc atggccgtgt cgcccgcgga gatgagcagg tgcgggtcgt
    15301 ccttcttgat cgcggcgaag ttcagcttcg cgtccgcgcg gatgttcgcc acgacagcga
    15361 tgcgcagcag cttcgatgga tagcctttga aggtgttgat gtccgaaacc tgatcaccac
    15421 tcttcacgcg gtagtgataa acgacatcct tctgcgggat cgggatctcc acatgatgca
    15481 gcgtgcggct gccgtccacc ttcgcgcttt caccaagctg tggcgatgtg ccaaactcga
    15541 ccacggaatc gccagggttg gtggtttccc aagagatgac gattttcgag ttgtccggcg
    15601 actggtgcgt catccagatg cgctcgactt tggcgaaagc ggaggtgctc aaaaaagcca
    15661 gtgtgagggc aaggagggtc ggtttcatgg ttgatgggct agctttacgc ctttcaagat
    15721 cgcaatattt ggaggccatg aagaataaga ttatatgttg aggtgctctg aatgagctgg
    15781 ccgctttcaa cagactcata ttcacggttt tgctgtggat gtttccgctc aggatggcac
    15841 ttcaagtacc accacctccg ctacttccgc gccatcaccc atgagggcag cctcacgaag
    15901 gccgcgaagc atctcaaact ctcgcaatcg gccctcagcg tgcagcttcg cagtttggag
    15961 gaaaacctcg gccagccgct gtttgagcgg aagcacaagg ccctcgtgct caccgaggcc
    16021 ggccagatcg cgctggaata tgccgactcc atcttccgca gcggcgagga actcaccgac
    16081 ctgctgcaaa accgcgccaa acgcagccgc ggcttcctcc gtgtcggcgc ggcgtcgaat
    16141 ttgtcgcgca acttccagct caccttcctg tgaccgctca tcacccgcga tgatgtcgag
    16201 ctcatcatcc actccggcac actgcgcgag cttttgaccc agctccaggc ccgcacgctc
    16261 gatgtcgtgc tttccaacac gcctgtgcgc cgcgatgccg agaccggctg gcacagtcat
    16321 ttgctcgacg aacaaacagt gagtctcgtg gggcacaaaa cacgcggcat gaagcccttt
    16381 cgctttcccg aggacatgcg caaaacgcct gtcgtgctcc ccagcctcga aagcagcatc
    16441 cgtgccccat ttgacgtcct catggatcaa accggcattc gtcccatcat cgcagccgaa
    16501 gtcgatgaca tggccatgct ccgcctcatg gcctgtgaaa cgaatggtgt caccctcgtc
    16561 ccgcccgtcg tcgttcgtga cgagttggaa agcggtgcgc tcgtcgagcg acactgtttc
    16621 ccccagatca aggagagctt ctatgccatt actccaagcc gccgcttccc gaatccgatt
    16681 ctccgtgagc tgatgaagcg gaagtgaaaa ctactgcctc ggcccataca cggaaatttt
    16741 ttcgcccgaa tacgcacccg cttcagtcag cgcggagaag tcaggtggct cgtagagcct
    16801 tgcggggtct tcggggagtg aaacggggac tttgagctcg cggccgtgtt tttcgaactc
    16861 ggtgcccgga agcttttgga aggtccagga gcggattttg ccttcaaagg gcatgccgag
    16921 gacggcgtag gcattgtggt cgatgaggca gtggtcgccg aagctctgcg catcgacggc
    16981 gcggcctcgt ccaggaccgt agccgccgta ttttgcgcct tcgggtggtt tgccgccgat
    17041 gatgaggttg tgggtgatca cggcgtgatc aaatggctcg ctggtgtgaa tgccgaggcc
    17101 gtcgccggct ttcacgacgg tgttgtgctg gatgacatcg ccctggctga aacggtgcag
    17161 tttgaagggc tcgaggatga tgttgaacat cacgttgcgc cggaagtagt tcggcccgcc
    17221 gagtccgggt tgcgaggaga tgccgacgaa gcagttcgtg aggcgattcc gcatcacgcg
    17281 gcagttgtgc agggcgaaat cggcctcgat gccgtcgtcg agaccggcgc tgaggtcgtt
    17341 gttgaggatg tcattgcaca tctgcggcgc cgcgccgtcg tcttcaatgt ggctgatgca
    17401 gtcgcggaag ccgctgacgc ggttgtggcg gatcacattg ccgctgccgg tgaactggat
    17461 gccctcgccc tcgttgtcgc cgcccgcgcc catgatctgc ggcttccact cgcggatgcc
    17521 ggtgatgacg ttgtcctcga tgcagccgtt catcatgccc ggcttgtaag ccttgatacc
    17581 aaactgcgag cggatgctgc aacgccgcac cacgcagtgc tccgagccat tcaagcgcac
    17641 cggcccgttc acggtgacgc cttcgagata cacccacttg cgattcgtca ggctgatctc
    17701 gctgaaaaca gcctttccgc tcgtgctgcg ataaacaatc ggccttccag gctccccatc
    17761 gcgattgaac gtcgccgcac cgtaatcgcc atccgccagc aaaagcacct cgccgggctt
    17821 cacggcattc aaatcgccct tcgagccgtt tttgaccacc gcatcggctt tggcgatggg
    17881 ttccggcctt gtcgaggctt tcacgatttt cgtcgctgaa ccgccatcgg ggtcatggag
    17941 cttcaattcg atctcgtagc tcgttcccgc ctccagatcg aagacagtgc cgctgagtcg
    18001 attcgcccag gtgaagatcg gcgaggtctt ttggctcttg ccggcaggca cacgccgcag
    18061 cgcctccgca ccgcgccaag tcgtttcgcc tgcttgacgc actttcacct cgcaggtggc
    18121 atcgagatca tcatcgccct cgatctgcca ttcgatggcg atgtggttga gggtcgggaa
    18181 cggcgtggtg acctcaccgg gttgcattga ttcagccgcg gagagccgca gtccaaaaag
    18241 gaacagaagg aggaagcgca tgcgactcag gcagttgttt gtcggggaca ggcccgaatg
    18301 gcacgggatt aaggcggcct ttcggcaaaa agtcgttgtg aatcaaggaa gtctggcgct
    18361 ttggagcatc tcaaagcctt gcagcgccga gagcttcaga gcttctcatg ccagcctgcc
    18421 aagcggacga aatcagccat tttcgtcgaa aacaggtctt aatcccgtgc cattcgggac
    18481 aggcccacag cagcgtgatg ccgatgagga agcagcccac gctatccatc aaggtccacc
    18541 acagcggcag cgggcattgc agatcaatca agagataggc tggacctgcg acgaccatgg
    18601 tccagccgca caggcgcacg agcggctggt agcgcgtgag atcgcgtgaa atggcccaaa
    18661 tcagcacggc gatggtcaca taggcgaagc cggcgttgcc ggaaaggtaa acgatgagcg
    18721 gtgtgatggg ctgtttgccg tagcccatga gccacgcgaa cttttggaag gcgatctccg
    18781 gcagcagcgc tcccggcaat cccgtgagca aaatggcaat catgaggcgc agcatccaaa
    18841 gctgcacgcg aggtcgggac tgacgggagg gattcatgcg gtttactttt ggcaggaaaa
    18901 tttgggggca agaaaattag cccgaacatc tgaatcccaa ttttcttgcc ctttaatctt
    18961 cttgcgaacc tcaacgcttc accgcgttgt catcggcccc attttcacga cttcgagatc
    19021 tttgcccttc actttgcgga tgggcaaaag ctcctcgtgg ttccagcgat catccggcgc
    19081 gccgctgatg aaccagtcgc cgccgttgtc ggcgaggatc atgccgtggg ttttgaggcc
    19141 ttcgaggatg actttggcgg ctccggtgaa cttcgtggtg tcaaagctgg ccttcaatcg
    19201 cacgcgcatt cccatcggtg ggaggttttc gtctttgtgt gggctggcga agtgcgtggc
    19261 gggcggcaca taggcgcggc ggcttttttt gacggtgaag cgcagcgcat gcgtgagctt
    19321 cttcgcgccg cagatttcat cgtagcgggc gagaccaggc agaatgggca aaccagccgc
    19381 atcagcgctt gtccagccag cgggacgcgg cttggggttc ttcaaatcga agatggctcc
    19441 gctgccggcc ttccacgact tgccaccatc cagcgggaag ctgtgaaaaa gctcgtatag
    19501 cttccagttg tccttgtcga gcacgagcac atggcggtcg ccctcgctct gcggtccgcc
    19561 ttcgatgtga gcattcggcg ggatcggata cggccccgga tcgctctcgt ccgcatactc
    19621 aaagctcacc ggcacctttg gctggtcacc gctgacgacg taatacggaa tgccgcccgg
    19681 ctggcccttc cacacgatgc caaagtcctc atgcaggccc gtctccgcgc caatggaggc
    19741 gatgatcgca tcgctgagcg gatcgacctc gtctttggag acatcgcgat tccatttgtc
    19801 attcgccggc atgagcaaaa cgccgccgag atcggcattc ggaccggttt tgagctgcgg
    19861 atgctgggcg agggccaagc tggtcagtga taggcagagg agtactttca tgaatggacc
    19921 ttcaaacgac aaaagtccga tcagctctct cagtaaactg ctgagtgttg gttcaccggc
    19981 tgagatacac ccatgtacgc tctttgagat cacgcgtctc gcctttttgc gtttcgcggg
    20041 tccagatgac gatggggcgc tgggagtagt tgcggtcgtt gatgccggac agtgtcagcg
    20101 tgctgtcgtg gcagatgaag gcgggcaaat caggctccca accgcggttc gcgtagatga
    20161 agacaccgct cacatccgtg ataccgccgt tcagtgtctg gatgatggtg ccgatcttcg
    20221 cggtcttcat gccgaggatc cagaggttgc cgccgtgatt gcggcacatg gtgccttcat
    20281 gctccacgtt gagttggcga caccacgcgc tctgcccggg cttttgcagg ttcaactggc
    20341 cgcagaggtc atcgacaaaa atatcgctgc tgccgtggcc ttcaacgcgg aagccgatgg
    20401 cgctgctgac gatgagcgtg cgcttggatt cgtggcgcaa ctcgatgttg tggctgccac
    20461 tgcgattgct catgcgttcg atgatgacgg cgggggcatc gttggaacca tcgacgagtc
    20521 gccatacggc cttgccggtg gtgtgaaagc gtccttccag gccgatgatg cgtcgcacgg
    20581 cacctcggat gatcacttcg ctatcgaaga agaactcctt gcccgcaggc aggtaaatcg
    20641 tctccgcgcc ggagtcgatg gctcgttgca aggcggcgct ggagtcctgt ttgccctcgg
    20701 gatcggctcc gaaggcttcg aggttcaccc acttgccatc ccacggtact tcgggagttt
    20761 ctttgactgg caagtgcgtg accttgccgg cctccgcgaa ggttttcagg ccttcaaaac
    20821 gaaacggcgc ggccacgttc gcatgagagg tgtcctcggt gatgcggcct gcttccttca
    20881 catcgccctt gtcacgacct ttgtcggcgt gatcgatgga aacgacgtgt ccggtgatct
    20941 cgacatctcg cagatacatc tggcgttggt tcagcaaacc gggcgcgtcg atgccggtgc
    21001 cggtgatttt ggagtccacg agcgtcaccg tgccccagga gtccttttcg ttgtagagcg
    21061 tcgtcacggc gttctcgctg gtcaggccgc gcacgaagat catctggtgg tagttgtgcc
    21121 agccaagctt gcgctgacct cgcagcgtga tgtgctccag cgtgttcgag ttcaccggcc
    21181 acttggtcac gatgccggtt tcaaagccct ccacggtgag gtttcgcacc agcagcggcc
    21241 cgatctcatc cgtgaagccc atgtcgagcc cgatgaggcc gctgtccttc gccgcacgca
    21301 gcgtcacgtt gcgagtcgtg ccctggttgc tcgcgttgaa ctgcatgcca atggcctgcg
    21361 gattggccgc gccgatcttg atcgtcaaat cgcgcaccgc attgcgaaac cgctgcgcgg
    21421 gcttcacgcc cgtccacagc accgccttcg gttggtcgcc aaaagctggc gtggagggcg
    21481 gcacgacgat tttcgtcaac gccatgcccg cgccttgcaa aatcgtccgc ttctcaccat
    21541 tcccgccctc gcccttcggc cagtggagcg tgtccgtgac gatccatatg cccgccggca
    21601 gatacacgat gcgatttcca ttcggaaacg catccagcgc cttctgaatc gccaccgtgt
    21661 catccctatc atcaactgcc atcgcaccaa actcccgcac atcgagcaca ccgccatctt
    21721 ttgggaagat gatcggcgtt tgcgccgcga ggctgctgat cgcaagaagg aggaggaagg
    21781 gtttcatgcg cgggtctttg aaacgtgcgg atgacaggaa gatgacaagc gggcggccga
    21841 tttcatcagc ggatcgtcgg cgagacagcc gcgagcgcgg cacgataaaa ctgccactgc
    21901 tcattgccca tgccgcgaag aagcggaagg gtttcatgac cgacgccggg caccccgata
    21961 aaggtgtggt caatgttcag cctcttcaga tggctggaga attcacggtt cagcgccgcg
    22021 gtgaagtcac gcgatcccgc ggccactcgc atgaacactt ttccgcgcac ggcggcggcc
    22081 tgtttctccg cgagggtcag cgggctttgg gcgcggaagt agtcgagatc gccgccgaag
    22141 gtcgtgcgca ggatgcactc ccgctcggca ggatttgctc tcgtgcgcgg gccttggaag
    22201 tcgagatcga gaggcccgcc cgcgaggcag gacgcggctc caaagacctc ggggaacttc
    22261 agcccaagcc gagccgcgcc gtagcctccc atgctgaagc cttcgacgat tcggccttca
    22321 cgtttcgcga aggtgcggaa ggtcgcgtcg atatgcggca cgagttcctt cacgacgagc
    22381 gtctccatcg gaaccgcgcc atccttggag tcacaccaca tgctcgtggc cagtccattt
    22441 ggaaacacga cgacgaccgg cggcattttg ccttcacgaa tcgccgcatc aaaccaggcg
    22501 gacaccggcg caatgcccgc gaggcctccg cccgtgccat gcagccagta gagcacggga
    22561 aagcgccgct ccttctccaa ttcgtaaacc tccggcgtgt aagcgtgaaa gctcaccttc
    22621 gattgaagcg cgctgctctc gaacgtgtga tgcgccacat gcggtgcctg caccgcaggg
    22681 gtcacccacg acgggccctg agccccggcg atagacacga acaatgcaaa gaagagcagg
    22741 aggatgagct tcatggtttc gaggttcctt tcgcggcagt ggcgggcttc agcgggtgct
    22801 tttggaaaaa atcccacatc aagtgattgg ccgagatgtc catggtggat gggcctagca
    22861 tctcaacgat gggtttcatg ctgggccagg tgtgtccgcc gccttcgatc tcgatgagca
    22921 cgacctcgct gccgtctttg ccaccgcccc aggtcttgcg cgtgcatttc atgccgtcgg
    22981 cggccttgtc gggcagtggg acgacttcag gcgtggcatt gcagccgttg tttttcaccc
    23041 aagtttggat tgtgtgctcg acggagcgga agtccgtcac gcccttcctt tgtccgaacg
    23101 atcctttgcc atagccgcct tgaaatggcg cgaaggcgtc cgcagtgccg tggaagtgca
    23161 tcacgggcac ggcgcgcttc gcgttcaccg cgtccatcat gagcggaccg ccgactggcg
    23221 cgatggctgc gatgcggtcg ctcagttcgg aggcgacgta atgcgacacg atgccgccat
    23281 tcgagaggcc cgtggcatag acacggttcg catcgacctt caccaccatg gcgagatcat
    23341 caagcagcgc cgccgtgaag gcgacgtcgt cgattttgtt ctccatcgca tagccgcagc
    23401 acccgccacc gttgaaggtg agcaactggt tctccaatcg cccggtgcca aaaggataga
    23461 ccacgatgaa tccggcctcc tccgacttcg cattcaaccc gctcagccgc accatgctcg
    23521 cgggattgcc accaccgccg tgaaacgcga tcaccaccgg cgtcgccttc gtcgaatcat
    23581 acctgcccgg cacatgaatc tggtagcgac gattcaattc gccgactttt accgtgcgcg
    23641 tgtgatctcc ggcggcgagc ggctccgcca tcgcggtggg ttcgatgctc aagaagcaag
    23701 cggccagcaa ggtcaacgtc cgcggcattc gacggtggtt tgtgttgagg gcgtgtgttt
    23761 tgggaaaggg cgttttcacg tcccgcaaga acacgccgcc gcaggttcgg ttgcgcgaag
    23821 ctatgtgaca aaaaatgttt ggttacggtt gtcatacacg aagcgtttgt tggacacaga
    23881 tcgtcatgca tcttctccgc tccattttgc tgataatcct ggtttccacg gccgtccagg
    23941 cgctcacgcc tgacgaagca gaggctcgtc tgcgcatggc gcggcagatc gcgcgggagc
    24001 ggaatgatcg ggagctggtg cagcaggtgg agaagctggc gcgtgagttc aaagcggggc
    24061 ttccggcgga ggcggaggag caattgcgcg aggcggagaa ggcagtgggc attgatccgg
    24121 gtggctggtc gatggccggg cagccgttgt ttcatcccac ggcggcgatg gaggccgcgt
    24181 tgaaggccga ggggccgaaa ctggcagcgg cgatggcttc gggcgatgcg aacgcagtgc
    24241 gcgagatcac gcgggtgatg gaaggcatcc ttggtgatca agcaggtgtg ccggatggcc
    24301 gccgcatggg gcagaagcct tctgaattga aactgagccg cgcggaggtg gtgaagctct
    24361 tcctcgccgc gctggagacg gagggccgcg cgatgcgcac cttgatgaag ggcgagcttt
    24421 tgccggatca aatggtccgc gtgtatgcct acgtgctcga cgcatgcgtg acgatgcatc
    24481 cgcatgtggc gcaacatgtg ccggaacgtg tcgcggatgt cgaaaagctg ctgcgaggca
    24541 cggcgagtgt gcttttgagg cttcagcagc cgcagggcca ttttccgttt ccggacctgc
    24601 gaggcaagaa catccgtttt ggcgacatga cgaccaaaca cctccaaaac ggcaccatcg
    24661 agatccaaga cggctggatc atcacgcctg atcccgatgg tggttcgcaa ttcgacacgg
    24721 gtgtgtgcgg cgtggccttg ttgcgggcgg gtgagctttt gaaggaggaa ggctatctcg
    24781 ctgcgggtcg tcgtgccgca gaatgggcgg cgaagcagaa atgctgcgcg aacttcaact
    24841 acaatgcctt ctccgtgtca ctgctggctc gtgcaggcat gaaggaggcc gcgttggaga
    24901 agtttcgtgt cggtgtcgcg cctggtcagg cgaagaacgg tcgctggctc gatgcgcaca
    24961 acgtgcgcac ggtttaccat gtgatcattc tgcgtgcgct ggccgatctc ggccgctccg
    25021 cagaggtcga tgcggtcgcg ctgccggcga ttcacgcctt gcttgatgaa ttcgatgcca
    25081 tgggcatcac ggtggaggcc ttgccggagc ttcatgcgct cgccaaacag catcctgacg
    25141 atgcgcggct gcaaaaagcc tttcgcgaca tggcctccac cctcgtcaac aaatgcaccg
    25201 acggcacacg cgtgaagatg ggcgcccaac cacatcaact cgccgcggtg gtcgaggtgg
    25261 tggagtgagg aagaactttt gagatctctt acactggctt catctccact ccggtgagcg
    25321 tgccggtgct ggtgccgaac ttgtcggtct cgatgcccat gcgctggagc atgttgacga
    25381 agagattgca gagcggcggc ggattgtcgg gatcgaaggc gagatgctgg ccgtgattga
    25441 aaccaccgcc ggcgagcagc acgggcagat tcttggtcga atggctgctg ccatcgccga
    25501 ggttgctgcc gaggaagacc gtggtgtgat cgagcaggtt ggagtcggcc tccttggatt
    25561 gttggagttt cgagaggaga tcgcgcacgg ttttcatcgt ctcctgctcg acgatttgaa
    25621 gctgcgagag cttgctggga tccttgccgt gatgcgagag gtcgtgatgg ccgagagaca
    25681 cgccctcaat cggcggcacc tgcgtgttgc ctgcgagcat gatggtgatg aggcgtgtgg
    25741 agtcggtttg cagcgcgaga tgactgagat caaagagcag gcgtgtgcga ccgatgagat
    25801 cggcggcatt ctgaatgtcc tgcggcggct tgacgtcgat ctttggcttt ggtttccggg
    25861 cccagctctc gtcgttgacc atgcgctgct ccagttcgcg cacgctggtg aggtattcgt
    25921 cgagcttgtc acgatcctcg ctgccgaggc cggagagcag cgatttcgcc tgcgagcgca
    25981 catcatcgag aatgctgcgg ccatcctcca gctggcgcat ttgctgcgcc acctcggcgg
    26041 cctcgccttc gaggaaaagc cgggcgaaga ccttcgaggg cgagttgtcc gccgggatca
    26101 aggcgccggt gcgtgtccat gacaggccgc cggaaccttc acccgacagc acgaggctgg
    26161 ggaagcgggt tgcggcgccg aggtgctcgg cggcgaactg gtcgagtgaa acggcattgc
    26221 gaaagcccgg cctgcccgcg ccaggagcgc cggtcaaaaa gctggccgag gcctgatgtg
    26281 caaagctgga gcccatgcct gcgtgctgca ggccggagat gactgtgaag tcatcgcggt
    26341 gatccttgag gctgtcgaga taggtcgaga gcgcgtagtc tttgcccgcc tgcttgggaa
    26401 agaaatgctc cggataaaag cccagcggcg cgcagatgca gatcatgcgc cgcggtgtcg
    26461 gcgaagcgct
//
LOCUS       DAOMYJ010000051        25796 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Prosthecobacter sp. isolate AMR_MDS_5629
            NODE_688_length_25796_cov_21.344004, whole genome shotgun sequence.
ACCESSION   DAOMYJ010000051 DAOMYJ010000000
VERSION     DAOMYJ010000051.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867850
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prosthecobacter sp. (wastewater metagenome)
  ORGANISM  Prosthecobacter sp.
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Verrucomicrobiaceae; Prosthecobacter.
REFERENCE   1  (bases 1 to 25796)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 25796)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.30
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:34:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,742
            CDSs (total)                      :: 4,684
            Genes (coding)                    :: 4,676
            CDSs (with protein)               :: 4,676
            Genes (RNA)                       :: 58
            tRNAs                             :: 55
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25796
                     /organism="Prosthecobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_688_length_25796_cov_21.344004"
                     /isolate="AMR_MDS_5629"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1965333"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            383..1018
                     /locus_tag="PLP58_07445"
     CDS             383..1018
                     /locus_tag="PLP58_07445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin type III domain-containing protein"
                     /protein_id="HRJ08385.1"
                     /translation="MAKVKLDLDDKNAEQVLAASGQHIAAMASTEGQALFPTPAPTVP
                     AYQAKHDALAAGINLVTTLRGQLTAALNALPGLTADLKAVMLERRDYVQDTTGGDPAL
                     IPVSGFQVAGDPGTPIGPLPAPQNVKAVPGSHPATVKVSCKAVKGAQFYPVEARPHND
                     PSAPWVQVSVNSTCRQTISGLISGVEYAFRIAAKGAAGISPWGDEAVCRAP"
     gene            1185..1601
                     /locus_tag="PLP58_07450"
     CDS             1185..1601
                     /locus_tag="PLP58_07450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ08386.1"
                     /translation="MFDPAYPPANAEIESAPLRLQLNSLHGLITAITTITAAQVDEVT
                     TLNPGEPATVSLSITGQTLRLSFGIPQGEPGGEGPPGNDGEVTQAALDAAIAGSASNV
                     NHISPLGMTAAGDYDPAQTQALADKLDELVSSLHRP"
     gene            complement(2001..2474)
                     /locus_tag="PLP58_07455"
     CDS             complement(2001..2474)
                     /locus_tag="PLP58_07455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ08387.1"
                     /translation="MNWLKSDLNCYQVVLPADTEPELVAQLAHFWRLYCHDVLSANSG
                     KQWDHLLISMRGGDGRAGIYQQVMAAESPVVAWSSVGIPWWEMIIYEMTTPDNPADLT
                     FENASNEQDERFARLIIEAAQQAGLARLAGREESGIRLRFHRYDDREPFMDVIIH"
     gene            complement(2510..2983)
                     /locus_tag="PLP58_07460"
     CDS             complement(2510..2983)
                     /locus_tag="PLP58_07460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ08388.1"
                     /translation="MEWLGFEFRCYTANLCGNEENNLVAELTEFWELFCQDVLPANAG
                     KTWDHLWITMRGIDGNFGIYQQVVESELTVGTWSSIHINWWQELVDDIPDSDDPNDLS
                     YRHSVRKLDEWIASLVVNAAQRAGLAKLAGREESGIRLRFHRYDNREPFMDVVVH"
     gene            complement(3224..5839)
                     /locus_tag="PLP58_07465"
     CDS             complement(3224..5839)
                     /locus_tag="PLP58_07465"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: protein motif:HMM:NF006032.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HRJ08389.1"
                     /translation="MPKALIIAEKPSVANDLARALAKAPGMTAFKKEKDYFENETHII
                     SSAIGHLIELAMPEANGKKIGWGWTHLPIMPEDFDLQPIEDSADRFKLLSRLMKRKDV
                     DRLINACDAGREGELIFRYIVEAAGVKKPVQRLWMQSMTQGAILDAYKKLRSDAEMLP
                     LADAAKCRSESDWLVGINATRALTALNSRNGGFRLTPAGRVQTPTLTILAKRELEIQA
                     FVPRTYYEVHALFEVPAGRYEGRWFDENFQKDEADEHKKAERLWDPAQAQAIVDRCIA
                     KPGKIEQVTKPAKQASPLLYDLTSLQREASNRFGFSARRTLQIAQALYEKFKVLTYPR
                     TDSRYLPEDYLGTVRGTLQTFASHDSRKHDALPHELGTHAATALDQGWVRPTRRVFDT
                     SKVSDHFAIIPTGQIPPKELPEAEQKLFDMVARRFVAVFFPPAEFEVTTRVTRVGQDA
                     FKSEGKIMIEPGWLAVYGKKAAAEATGQPGENKDKIIAAAKDGDTASTIDAKAEELVT
                     KPPARFNEATLLSTMENAGKFVDDEELAEAMSERGLGTPATRAAIIEGLIHDRYIERE
                     GRDLVVTAKGLALIDQISRIGIEALSSPELTGQWEYKLRQMEHNQLDRASFMRDIRSL
                     TTSVVEKTRAYLKIAKDRVFPDFDACCGLCGQRGFKQSEDHLTCKNPDCKVRVYKTIA
                     GRTLDDDEMRGLLENRFLPPREGFRSRKGQDFTAGIEIKDDGKTAFVFPGGQNDPDAP
                     PPFDFGSAQPIVPVCPVCAKKGRKGAIYDTPDNYICSTAAADPKACNARLPKMLCKKD
                     ITAENAVLFFTEGRTALIEGMISKRGRPFKSFLVCNAGDKRLLGWEFPPREARPKAPG
                     GGGKKSRFTKKDAEE"
     gene            6084..6209
                     /locus_tag="PLP58_07470"
     CDS             6084..6209
                     /locus_tag="PLP58_07470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ08390.1"
                     /translation="MKHTTLLLALAAAFTFASCKKAEEAPKAEEPKAAEKAPETK"
     gene            6350..8929
                     /locus_tag="PLP58_07475"
     CDS             6350..8929
                     /locus_tag="PLP58_07475"
                     /inference="COORDINATES: protein motif:HMM:NF019334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HRJ08391.1"
                     /translation="MAERYKVYDQLGAGGVGAVYRAYDNELKRWVAIKRLMSSADAGG
                     DAKLAAELRREADALASLRNPNIVTIFDVSSDGEGLFMVMELLEGEDLADVVGRGPLH
                     YDDFKELASQTLEALLAAHQRHILHRDIKPENIKVERLPGGRMQSKIIDFGLARAGLR
                     ARKQTEDVTGSVMGSVFYMAPEQLTREPVDERTDLYSLGAVFYEALSGRKAFDGDTLA
                     VVIDKHLNHDTLPLHVVAPHVPPWLGAWVLRLMALKPEDRPVNAQQAIEEFRAWEKMA
                     SAPPMMPWMPMGYGYSPPVQPGTGQVYPGTAPAPVQTGYVQPVLEVIPTARPVVEVAA
                     RTSAITGADTARQVVRPGGNRSSALARPAQKSASAASGSPPDKKKLFIAIGAGVAVLL
                     LAFFLFGGSSGEDDRPDTAAKSAPVPPPSAGSSKILFQLPESRVFPPLSDDIVMHYIA
                     TTGVSSGRKGSDGKPALVAVNSQASAWHDLAARGDDNVLETPDRKPAAGIRRVTWTTG
                     DGFSGIKDGRNALNFTSEGGKDAVLAFEAPGSKAAHMPFGQAARKGDAGLTLALVFQA
                     QAAALPMRVLALEADKDNALLLGVNSQRQITAEFRAGGRSETLAAPHLNATRPCAVLI
                     AWNADGTILLRAREAAGRGVSVEKKGHSAPGKALKGLRIGGGEQGASTFQGLLGELIV
                     YASALKQDQAQLVAGSQIPGFYFKGTTPPNLPKVTALTERLSIKKPRVGPRTDWAVNT
                     SHLPEDAWLAIDGNTATRWRTDTPQKSGQWFQVTLKEDREISGLALDVSCSPDEHPRR
                     YEVETSLDGKSWSRAASGDNDKPLLEIAFPQPVKTRHVRVKQTGGAPSAHWSIAELEV
                     FAK"
     gene            9018..9842
                     /locus_tag="PLP58_07480"
     CDS             9018..9842
                     /locus_tag="PLP58_07480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ08392.1"
                     /translation="MNPPFAFRPAFGALLLAFAAPALVSAQGGKSGVQEFDPRAFPGQ
                     LVDDVVVPVPSEVFTVLDKLGSPNWRQEVRKEKPPKTGDRTRLALLFGLTVAEGFVAV
                     QAEDKESVKDIGRDVIDLSTALGLIKAVRPHAQAILDAADAGDWATIRKEFDKTQKTV
                     RDSMEQMKDNDLSQCVSIGGWLRGTASVTSVVTKNFSADSAELLNQPMLVEHFISRIE
                     RMSKDKKDHDAITDIAKGLRGILGRMEGAVDGFTRDAVREIGKICETLLGEIKAAK"
     gene            9883..10335
                     /locus_tag="PLP58_07485"
     CDS             9883..10335
                     /locus_tag="PLP58_07485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ08393.1"
                     /translation="MNFRLPTLRRACAFITLAVLLFQSPSALATIDDAHSFAMEAAIP
                     FVKKGFIVREDYWNGEVKSGQRLMVKHQMFKGNEYAFWLGCPEDDAVFELLVLDEKGN
                     EISLDFNASQYFASARVNPPKTGTYTVIFSVSSKRAKSLYWALAYGYH"
     gene            10344..11336
                     /locus_tag="PLP58_07490"
     CDS             10344..11336
                     /locus_tag="PLP58_07490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007418587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HRJ08394.1"
                     /translation="MPVETLTYETPQFLQKLIGHDLGGLRVIAAGTETQITSRDAWVR
                     IEGADAAAVGAAREVLTQLERLRRQGGEITSAMIGLVTESVLTGHDDAAPASSILTLK
                     LAGSAKKPPVLAKTRGQIAYVQAMRDHEIVFGIGPAGTGKTYLAMAQGLHYLREKTVQ
                     RLVLTRPAVEAGEALGFLPGDLKEKIFPYLRPLYDALYDMLEPDEAERMIERGAIEIA
                     PLAYMRGRTLKNAFIILDEAQNTTTEQMLMFLTRLGEGARAVVTGDPSQVDLRRHVRS
                     GLREAVEVLQGVEGVRFVQFLPKDIVRLPVVQRIIEAYDRRRAGGESRGAADGA"
     gene            complement(11402..13624)
                     /locus_tag="PLP58_07495"
     CDS             complement(11402..13624)
                     /locus_tag="PLP58_07495"
                     /EC_number="2.7.10.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045226 - extracellular polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis tyrosine autokinase"
                     /protein_id="HRJ08395.1"
                     /translation="MNTTQTISMDQPLVLPGQAQSHQLNRIHETSARLQRYQILFRRR
                     WWFLLLTASIGVCVAAVIVTGRPEEYKSVAKLVAGGRMTTQGDFTWVEQLQDFYGTII
                     DTVQSPEMKQRALARIRALYPDLKDSEVRIQVVQNRGSAIFNVFATGQDQEYPKIFLQ
                     ALMDEFIAFRQNIREQGLERALNTFIEDFVKRGRELQDRGEKLEAFRKANNVVLLTNG
                     NNDAAAFLQSLRIKKEALITELNELRLALEDVDIAMVNRERAVLGGQAAPGAVTAAQG
                     GEAAGVTPKVEGGRSSLSMSVAERNYLEVRNEVFKLNNERANLLKSFKEQHPAVVALD
                     ERIESAKRLLNDFQDVILKEFRGQISDMERRQRVLDEQIAIKEKEALELGAKLAEHER
                     LEEEWKAAKLAHDQMAETVTKFQQMTNIQTDYVAVQEPASEVYQEEQKLLVPLTGGLL
                     AGLAGGMLVLLLFDRLDDRMNSFAEFQALFPGEAVLGQIPEQKQRGDVALLQANDTRH
                     LYAEAFRNVRSSILFKNWQGNPPKTILVTSAVPNEGKTTVTSNLAITMALGGARVLLA
                     DCDLRRGGVSELFKLPNTPGLSEVLRGQVSWRDALQDTGIPNLKLLARGEVIDQTSEV
                     LLSKTAETVLREMAADYDYVIFDSAPVLVADDTGSFAPKLDTVLFVVRMSSTMARLSG
                     KALDTLYERQVNVGGVILNRSSSNLKEYTYYNYASYYYTPAARAAAKEGQSKEPAAKA
                     "
     gene            complement(13742..14464)
                     /locus_tag="PLP58_07500"
     CDS             complement(13742..14464)
                     /locus_tag="PLP58_07500"
                     /inference="COORDINATES: protein motif:HMM:NF014606.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015159 - polysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015774 - polysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis/export family
                     protein"
                     /protein_id="HRJ08396.1"
                     /translation="MRTLPLIAFSALLPAFLTAQDPGVREFEDRRQTSPAPVQTGDIA
                     RSAAIINSMDVLDDSRPIEPGDTISMRIVEDRTEPRQLTVGVTGEVYAPHIGLVKAAG
                     RTSKQLAYEVKRRLEASYYNKATVIVAIDRQRQDDPNARLRYSSSEIDFFTVYGQVLR
                     QGKYELPLDEDITISQAILRAGGFAQFANHEKVKLVRKTPQGNKTIIINLDTIMRKGN
                     LEYDVYIRNNDVIIVDEKKINI"
     gene            complement(14474..16198)
                     /locus_tag="PLP58_07505"
     CDS             complement(14474..16198)
                     /locus_tag="PLP58_07505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ08397.1"
                     /translation="MAAAAVLTLTCVEVQAQYGVSPSQTGMFFDDLRARQRAESAASA
                     SGARDGNVVYDYDSGIYRATGNEMGQRSTDYQSAREVSRPSIRSGSNAFSARTAGDYT
                     NYAGQYTSPTGFFAPTYISDPFLSGRRNVRLGPVNVGFGFYQGIEYNDNVNRSGVDPV
                     SDVITSTLLNIDANYQVTQNNRLSISTALGFDNYWNHPELSPYGDGNFIVNVLPGSTI
                     AFDIKAGPVYFTVYDRISVRPAVRNDFALTRNQVFGVFQNDVGVAANWRINSAWSLSV
                     NLMHSDSQALGGGQTGTVGVTDEDNFSRTTDSIHASLTFSPTGTWVLGMEGGVTDMQY
                     DQEFNADGVLSNIGAFVVLPVAKSTYIRVAGGWQNFEFDEVGFKSLGTTPLTSGDTSD
                     LSDFYYSVTLSNQLNSRVSHSLTFGREAALNVTSNYVVADYLNYGISFIAWKGSRIAL
                     SAYVEDAEMSGGVFAQETLQYGMDIHVSHRVTSKLTAGVGYHYGRTDTDRMGSSNALG
                     SLGSAANFGTFDQHAWNVDLTYALNHKASLIFGYRYYLTDMLSGPGRFNDQDFTQNRL
                     IMGFNYNF"
     gene            16507..17622
                     /gene="ychF"
                     /locus_tag="PLP58_07510"
     CDS             16507..17622
                     /gene="ychF"
                     /locus_tag="PLP58_07510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009965890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HRJ08398.1"
                     /translation="MLQAGIVGLPNVGKSTLFNAVTRTRKAEAANYPFCTIEPNQGVV
                     IVPDERLAVLSKLSGSQKLVPAAIEFVDIAGLVKGASQGEGLGNKFLSHIREVDAIVH
                     VVRCFESGDITHVDGSVDPVRDIEVINTELILADLEAVTRRKERCEKKAKSGDKESKA
                     ELALCEKLLPHLDAMKPALTADLSDDERKLMKSFFLLSGKPCIYACNVAEADLASASK
                     EPDKHPLVSKVREYASHAHAASACVVSAAIENELVDLPDEDASAYLADLGVEDSGVNQ
                     LIKSVYSLLGLQTYLTTGEKETRAWTITKGMKAPQAAGVIHGDFERGFIAAQIVSFED
                     LVACGSEAKAREAGKLRIEGKEYVMRDGDVVEWRFNV"
     gene            complement(17701..18198)
                     /gene="rplI"
                     /locus_tag="PLP58_07515"
     CDS             complement(17701..18198)
                     /gene="rplI"
                     /locus_tag="PLP58_07515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009963203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HRJ08399.1"
                     /translation="MAHAQVILKEHIKGLGAEADVVKVKRGFARNFLVPQGKAYDATP
                     GNLRHLNRLKAVRAEREAKELQEAEKVAAKLKKLKLKLTLQTGQGGKAFGSITTIDIA
                     RAIAESAKIELDRHQIELDKPIKSTGAFEVPVKLHAEVSCVVKLNVSAAEGAESPESE
                     EADAD"
     gene            18407..19183
                     /gene="kdsB"
                     /locus_tag="PLP58_07520"
     CDS             18407..19183
                     /gene="kdsB"
                     /locus_tag="PLP58_07520"
                     /EC_number="2.7.7.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012419228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008690 - 3-deoxy-manno-octulosonate
                     cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate cytidylyltransferase"
                     /protein_id="HRJ08400.1"
                     /translation="MNLPVTALAATDSDRTLVAIPARWGSTRFPGKPLHLIAGKPLVQ
                     HVWERCQQCRNVDEVIIATDDARIAEAAASFGAKCALTAPDHPSGTDRIAEAARAFPD
                     HRVIINVQGDEPLISPALIDELATVLVNEPQVRMITAAAPVLDPAHITDPNIVKVVFD
                     THGDSLYFSRSPLPFIRDASAGARHYRHLGIYGFQRGFLFQFVAWPPSRLERAEALEQ
                     LRALENGVRLRVVLTDEISPGVDTPEQAAAVERQLASAVL"
     gene            19216..20835
                     /locus_tag="PLP58_07525"
     CDS             19216..20835
                     /locus_tag="PLP58_07525"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012419229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003883 - CTP synthase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006241 - CTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HRJ08401.1"
                     /translation="MKYIFVTGGVVSSLGKGLAASALGTLLELRGLRVIMQKFDPYLN
                     IDPGTMNPYEHGEVYVLDDGAETDLDLGHYERFTHTNLSRLNNLTSGQVYMSVLEKER
                     AGAYEGRTVQVIPHVTNEIKDRIREVAKNMPGDIIITEIGGTVGDIEGLPFLEAIRQF
                     AIEAGQGNVLFIHATLVPYIKAAQELKTKPTQQSLAKLREIGIAPQIILCRTEHPLDL
                     DVREKISLFGNVPIEDVIEVRDVKHSIYEVPLKLHEQRFDDRVCKVLHIESPQPDLSK
                     WRHFVQRIIHPTHHVRIGVVGKYIELQDAYKSIYESLTHAGAASDCKVDIVKVDAEAI
                     EKGGPDLYLNGLQGILIPGGFGDRGTEGKIAAAGYARQHGIPFFGICLGMQIAVIEFA
                     RNVCGLAQASSTEFDKGTPHPVISMMEEQKKIRHLGGTMRLGNWVTRLVPGSKAHALY
                     QSDTIEERHRHRYEVNEDYKDTLESRGMIISGVSAKGDLAEMVELPAHPYFVACQFHP
                     EFLSKPNHPHPLFHGFVQAAMKHRIVPEPHC"
     gene            complement(20869..21465)
                     /locus_tag="PLP58_07530"
     CDS             complement(20869..21465)
                     /locus_tag="PLP58_07530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018970139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ08402.1"
                     /translation="MEHVLVIPRALFDSLGTFQGFQPDADRFLQAMLAPGANFFMERP
                     AAETDPSHKQLIPYSIFHHAGRYLCYQRGGSSGEKRLVARRSLGVGGHINPVDQSCDG
                     MGMTMYFNSLERELAEELVLTGARSQRVIGLINDDSSEVGSVHLGVVHLFDLDNDDVA
                     SNEDAIHDPRFMTLDELRSRRDELETWSQICLDQLPAA"
     gene            21579..23225
                     /locus_tag="PLP58_07535"
     CDS             21579..23225
                     /locus_tag="PLP58_07535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009959870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="altronate dehydratase family protein"
                     /protein_id="HRJ08403.1"
                     /translation="MTPDSPVLRVNEGDDLIVALRDLKAGSVVTLGGECWTLREDVPA
                     KHKFAARDFAAGERARMYGVVVGKTTQTVPAGALLRTSNLAHDAAAFAGKQRDAAWTA
                     PDVSKWRGRVFQGYRRGAGIAGTANHWLVIPLVFCENRNLAVMREALTRALGYAHGSA
                     YERYAAHLAEARHRGVPATLGLDDCFGAESTPLFANVDGVKFLEHGLGCGGTRQDAEA
                     LCRLLAGYIAHPNVAGATVLSLGCQHAQASLLESALAELHPGHDRPLQIFEQQKSASE
                     RDMLGRAIQSVISGVAKASEQTRESCQVSDLVLGVECGGSDGFSGISANPVIGRAADL
                     LAACGGTPVLAEFPELCGVEQELCDRCASRELAEKFTRLMRAYEAAARACGSGFEANP
                     SPGNIRDGLITDAMKSAGAAKKGGTAPIVDVLDYGEPVRRRGGLNLCCTPGNDVESTT
                     ALAGAGCNLIVFSTGLGTPTGNPVCPTLKVATNTGLMRKMPDILDFDAGPVITGESTL
                     EDLGERLFEACLAVASGTLQPKAVALGQDDFLPWKRGVSL"
     gene            23336..23785
                     /locus_tag="PLP58_07540"
     CDS             23336..23785
                     /locus_tag="PLP58_07540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ08404.1"
                     /translation="MTRPAEPLLYLAEPAPAVRKSPGVVKISLFDHFVHFTIFLFLVG
                     GCALLLDQASARTTGHEAQAGHWFAHGWAALSTFGSLFGKYTALFGASLVSTALMVAM
                     VQSLRLLDVMHCRNVAAALREKPGGGTQVTRAMARDGGYYMLAPVFE"
     gene            complement(23793..23867)
                     /locus_tag="PLP58_07545"
     tRNA            complement(23793..23867)
                     /locus_tag="PLP58_07545"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(23833..23835),aa:Lys,seq:ctt)
     gene            complement(23888..23962)
                     /locus_tag="PLP58_07550"
     tRNA            complement(23888..23962)
                     /locus_tag="PLP58_07550"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(23928..23930),aa:Lys,seq:ttt)
     gene            24123..24401
                     /locus_tag="PLP58_07555"
     CDS             24123..24401
                     /locus_tag="PLP58_07555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009963430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ08405.1"
                     /translation="MNTWTPPNPVNLADLGFMDARSKLIDLAAFLDRVQRAGQDDDFR
                     VQALKKAAGLLSLDQPTRAREVLLSFSDPGAEPVEKAAMQGALGAFKV"
     gene            24408..25214
                     /locus_tag="PLP58_07560"
     CDS             24408..25214
                     /locus_tag="PLP58_07560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018968958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HRJ08406.1"
                     /translation="MNYIEPHGHMVSRTTDDYERMALAGCRAICEPAFWAGFDRASAQ
                     GFYDYFRQITEYEPKRAAKFGVAHYCWLCINPKEAEDLKLAEEVIGIIPEFLGKPGVL
                     GIGEIGLNKNSRNELRIFERHLQVAQEHGQLVLIHTPHLEDKLKGTRLIVEALKNFPA
                     IRPERVIIDHVEEHTIDHVLDHGFWAGITLYPESKCTPHRAIDMLEQRKAARIWMNSA
                     CDWGVSDPLAVPKTMQAMRQRGWSREMIQRVAWENPVAFMRQCPKFQPPA"
     gene            25239..>25796
                     /locus_tag="PLP58_07565"
     CDS             25239..>25796
                     /locus_tag="PLP58_07565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ08407.1"
                     /translation="MTRALSYRDFSIGLALLGVTAFFAVVEPKFVDSRNLSLLMTELS
                     ITATLAMGMLLVILPGHIDLSVGSGLALLSGIATVLTARAGLPSPVALGIAFAAGLLV
                     WWAKGWLIVRERIPAFIVTLAGLLMFRGLFWLVIQNQTIPVVPGGQTNLYSLLSTWYL
                     PPPLGWAFAALVIAALGFCAWRGRRS"
BASE COUNT         4790 a         8212 c         7985 g         4809 t
ORIGIN      
        1 ctgccgcctg tttcctcaga actgccgacg gtttccgcaa aaccacagac ggttttcgca
       61 gaactgccgc ctgtttccac agaaccgccg acggtttcca cagaacagcc gacggtttcc
      121 acagaaccgc cggcggtttt cgcaaaactg ccggcggttt ccagcgcgct tcacgcctat
      181 ctcgcaggcg ggagtgcttc attttcagcc agtcacgccc tgtgcggcgg cgtgcctgac
      241 tccccagcgc cggggcaggc gcatccgccg ccaacaccga acccaggggg tagccatgcc
      301 tcgttttgac tccggcgctg cctatgacag ctccgctcac tacgatgagc cgccgccagc
      361 ccccgcttcc ccgaaaaaac atatggccaa agtcaaactc gacctggacg acaaaaacgc
      421 cgagcaggtg ctcgccgcgt cgggccagca catcgccgcc atggcatcca cggagggcca
      481 ggccctcttc cccacccccg cgcccaccgt gcccgcctac caggccaagc acgacgctct
      541 ggctgcgggc atcaacctcg tcaccaccct ccgcggccag cttaccgccg ccctcaacgc
      601 cctgcccggc ctgacggcgg acctcaaagc cgtcatgctg gagcggcgcg actatgtgca
      661 ggacaccacc ggcggcgacc ccgccctcat ccccgtctcc ggcttccagg tcgcgggtga
      721 tccgggcacg cccatcggcc cgctgcccgc cccgcaaaac gtcaaggccg tgcccggcag
      781 ccaccccgcc accgtcaaag tgagctgcaa ggccgtcaaa ggcgcccagt tctaccccgt
      841 cgaggcgcgc ccccacaacg acccctcagc cccctgggtg caggtctccg tgaacagcac
      901 ctgccgccag accatctccg gcctcatctc cggagtggag tacgccttcc gcatcgccgc
      961 caaaggcgcc gccggaatca gcccctgggg tgacgaggcc gtctgccgcg cgccgtgacc
     1021 ttcggagggg agggcggaca cccgggtccg ccctccatcc gaaggccaaa gggtcaaggg
     1081 cgaagtccgc gcctcgcagc tccaaggttc aagcctcact tttcaagccg ggaatttcga
     1141 gcttcggaat tcctccgttc cgcaatccgc attccaccat ccttatgttc gaccccgcct
     1201 acccgcccgc caacgccgag atcgagtccg cccccctgcg gctccagttg aacagcctcc
     1261 acggcctcat caccgccatc accaccatca ccgccgcgca ggtggacgag gtcaccaccc
     1321 tcaaccccgg agagccggcc accgtcagcc tcagcatcac cgggcagacc ctgcgcctca
     1381 gcttcggcat cccccagggc gagccaggcg gggaggggcc gcccggcaac gacggggaag
     1441 tcacccaggc cgcccttgac gccgccatcg ccggcagcgc cagcaacgtc aaccacatct
     1501 cccccctcgg catgaccgcc gccggcgact acgaccccgc ccagacccag gccctcgccg
     1561 acaagctcga cgagctcgtc tcctccctcc accgcccctg aggccgcgcg cccccgcgca
     1621 ggggaatggc cgcggcatcc gggcacaacg ccggatgccg catcaagatg agggaagccg
     1681 tatccgaccc cggaaaaaaa gcagccggaa cgcgagccaa gcagcggcgc gtgtggcaac
     1741 ccgcggcatt gcggaaagcc gcgcactctt ttgcggcagg tgcgggagca ggcggagctt
     1801 gtcagtggtg ggcaaggcac ggagccgcga gctttgcgaa gcgctggagt tgcggggccg
     1861 gttgcctgct gcgtgacgag tcatcggcct tgccgatgac tgcccagctc aggcacgggc
     1921 ggtctcgtga gacattgcag gcttgggtgc aggctaatag gaaggcaagt tgtcagggtt
     1981 gtcgcctgga tccgttttcg ctaatgaatg atgacatcca tgaatggttc gcggtcgtca
     2041 tagcggtgaa atctcaagcg gatgccggat tcctcacggc cagcaagtct tgccagccct
     2101 gcttgttgag ccgcctcaat gatgaggcgg gcgaaccgct catcctgctc gttggaggcg
     2161 ttctcaaacg tgaggtcggc gggattgtct ggagttgtca tttcatagat gatcatttcc
     2221 caccaaggga tgcccacact tgaccaagct accacaggag attccgcagc catcacttgc
     2281 tggtagattc ctgcgcgccc atcgcctcct cgcattgaga tcaataagtg gtcccactgt
     2341 ttgccagagt tggccgataa gacatcatgg caatacaatc tccagaaatg tgcaagctgc
     2401 gcgaccaatt ccggctcagt atcagccggt agcactactt gataacaatt caggtcagac
     2461 ttgagccagt tcatagtcac ggaatccaaa gatgaaacaa actgctccgc taatgaacca
     2521 caacgtccat gaacggctcc cggttgtcat agcggtggaa acgcaggcgg atgccggatt
     2581 cttctcgtcc agcaagtttt gccagccctg cccgttgtgc tgcgttgacg accaggctgg
     2641 caatccattc atccagtttg cgcacgctat gtctgtaaga caggtcgttc ggatcatccg
     2701 agtcaggaat gtcatcaaca agttcttgcc accagttgat gtgaatggag gaccatgtgc
     2761 cgacggtcaa ttctgattcg acaacctgct ggtaaatgcc aaagttacca tcaatgccac
     2821 gcattgtgat ccatagatga tcccatgtct tacccgcatt agctggcagg acgtcttgac
     2881 aaaacaactc ccagaactct gtgagttcag caacaagatt gttttcctca ttgccacaaa
     2941 ggttggccgt gtagcaacga aattcaaatc caagccattc cataaattgc gaagggttga
     3001 tcagagtatg gcaggtgcat aaaagcagcc aggcaaaaag cagctttcaa gaatgccagc
     3061 cattcacaat gaatgtctca ttggcattga atgaaatcgc attgtgccgc gcgtgcgcgg
     3121 catgagttgc ggtgtttgca gttcgatgaa tgagcctttc gtgccgttcc cggcaagcct
     3181 gcgcctcggc gcttgatgcc tggcattccc ggcggtcgcc ggctcactcc tccgcgtctt
     3241 ttttggtgaa gcggcttttc tttccgcctc cgccgggagc cttgggcctg gcttcgcgcg
     3301 gggggaactc ccagccgagc aagcgcttgt caccggcgtt gcagacgagg aaggatttga
     3361 aggggcggcc tcttttggag atcatgccct cgatgagcgc ggtcctgcct tcggtgaaga
     3421 agaggacggc gttttcagcg gtgatgtcct ttttgcagag catcttcggc agcctggcgt
     3481 tgcaggcttt gggatcggcg gcggcggtgc tgcagatgta gttgtcgggt gtgtcgtaga
     3541 tggcgccttt gcggcctttc ttcgcgcaga cggggcagac ggggacgatg ggctgggcgc
     3601 tgccgaagtc gaagggaggg ggggcgtcgg ggtcgttctg gccgccgggg aagacgaagg
     3661 cggttttgcc gtcgtccttg atctcgatgc cggcggtgaa gtcctggccc ttgcggctgc
     3721 ggaagccctc gcgcggcggg aggaagcggt tttcgagcag cccgcgcatc tcgtcgtcgt
     3781 ccagggtgcg gccggcgatg gttttgtaga cgcggacttt gcagtcgggg tttttgcagg
     3841 tgaggtggtc ctcgctctgc ttgaagccgc gctggccgca gaggccgcag caggcgtcaa
     3901 agtcggggaa gactcggtct ttggcgatct tcaaataggc gcgggtcttt tcgacgacgg
     3961 aggtggtgag ggagcggatg tcgcgcatga agctggcgcg gtcgagctgg ttgtgctcca
     4021 tctggcgcag cttgtactcc cactggccgg tgagctcggg gctgctgagc gcctcgatgc
     4081 cgatccggct gatctggtct atgagggcga ggcctttggc ggtgacgacg aggtcgcgcc
     4141 cttcgcgctc gatgtagcgg tcatggatga ggccctcgat gatggcggcg cgggtggcgg
     4201 gcgtgccgag cccgcgctcg ctcatggcct cggctagctc ctcgtcgtcc acaaatttgc
     4261 cggcgttttc catggtggag agcagggtgg cctcgttgaa gcgggcgggc ggcttggtga
     4321 cgagctcctc ggctttggcg tcaatggtgc tggcagtgtc gccgtctttg gcggcggcga
     4381 tgattttgtc cttgttttcg ccaggctggc cggtggcttc ggcggcggct tttttgccat
     4441 acacggcgag ccagccgggc tcgatcatga tcttgccctc gcttttgaag gcgtcctgcc
     4501 cgacgcgggt gacgcgggtg gtgacctcga actcggcggg cgggaagaag acggcgacaa
     4561 agcggcgcgc gaccatgtcg aagagcttct gctcggcctc gggcagctcc ttgggcggga
     4621 tctggccggt ggggatgatg gcaaagtggt cgctgacttt gctggtgtcg aagacgcggc
     4681 gggtgggccg cacccagccc tggtccaggg cggtggcggc gtgggtgccg agctcgtgcg
     4741 gcagcgcgtc atgcttccgg ctgtcatggc tggcgaaggt ctggagggtg ccgcgcacgg
     4801 tgccgaggta gtcctcgggc aggtagcggg agtcggtccg cggataggtg agcactttga
     4861 acttctcata gagcgcctgg gcgatctgga gggtgcggcg ggcggagaag ccgaagcggt
     4921 tgctggcctc gcgctggagg ctggtgaggt cgtaaagcag gggggaggct tgtttggcgg
     4981 gcttggtgac ctgctcgatc tttccgggtt tggcgatgca gcggtccacg atggcctgag
     5041 cctgggcggg gtcccagagg cgctcggctt ttttgtgctc gtcggcctcg tctttctgga
     5101 agttctcgtc gaaccagcgt ccctcgtagc gcccggccgg cacctcgaag agggcgtgaa
     5161 cctcataata ggtgcgcggg acgaaggcct ggatctccag ctcgcgcttg gccaggatgg
     5221 tgagggtggg cgtctgcacg cggccagcag gagtgagacg gaagccgccg ttgcgggagt
     5281 tcaaagcggt cagagcgcgg gtggcattga tgccgacgag ccagtcggac tcgctgcggc
     5341 acttggcggc gtcggccagg ggcagcatct ccgcgtcact gcgcagcttt ttgtaggcgt
     5401 cgagaatggc gccctgggtc atggactgca tccagaggcg ctggacgggc tttttgacgc
     5461 cggcggcctc gacaatgtag cggaagatga gctcgccctc gcgcccggcg tcgcaggcgt
     5521 tgatgaggcg gtccacatcc ttgcgcttca tgaggcggga gaggagcttg aagcggtccg
     5581 cgctgtcctc gatgggctgg aggtcgaagt cctccggcat gatgggcagg tgggtccagc
     5641 cccagccgat ttttttgccg ttggcctcgg gcatggccag ctcgatgaga tggccgatgg
     5701 cgctgctgat gatgtgggtc tcgttctcga aatagtcttt ctcttttttg aaggcggtca
     5761 tgccgggagc cttggcgagc gcgcgggcta ggtcgttggc cacgctgggc ttttctgcga
     5821 tgatgagggc cttgggcatg atgaaaatgg gggggggacg ggcggcaatg aagggcacgc
     5881 tttccagccc catgtcaatc attagctgcc ctgcgggcct ggatggccgg gtcacatgaa
     5941 gcgagttcgc agcccgcatc cgggctggct cccctcatca agcgggggaa tttgcaaaaa
     6001 caagaatggc atgatccgcg accatgaaag tgtcgaagtt agcgcttgcg gcatcacggg
     6061 ccgtcatcaa ttctaaagcc cctatgaaac ataccaccct ccttctcgcc ctcgccgcgg
     6121 ctttcacctt cgcctcctgc aaaaaggctg aggaagctcc caaggccgag gagccgaagg
     6181 ccgccgaaaa ggcgcctgag accaaataag cagcccgcaa gggttgaccc gaatcgtgga
     6241 gcgcaggcgc caaacgcctg cgctctttgc tttttaagaa tttgcgaaga tcgttcttgc
     6301 caagcgcccg cgctttggaa tcctggcggg tcaaactccc acacaccgca tggcggaacg
     6361 atacaaagtc tatgatcaac tcggcgcggg cggggtgggc gcagtgtacc gcgcctatga
     6421 caacgagctg aaacgctggg tcgccatcaa aagactgatg agcagcgccg acgccggagg
     6481 cgacgccaaa ctggcggcgg aactgcgccg ggaggcggac gcgctggcgt cgctgcgcaa
     6541 tccgaacatc gtcaccatct tcgatgtcag cagcgacggc gagggcctct tcatggtcat
     6601 ggagttgctg gaaggcgagg atctggccga cgtggtggga cgcggcccac tgcactacga
     6661 cgacttcaaa gagctggcct cgcaaacgct ggaagccctc cttgccgccc atcagcgcca
     6721 catcctgcac cgcgacatca agccggagaa catcaaggtc gagcgcctgc ctggcggtcg
     6781 catgcagtcg aaaatcatag atttcggcct ggcacgcgcc gggctgcgcg cgcgcaagca
     6841 gacggaggat gtgacgggat cggtgatggg ctcggttttt tacatggctc ccgagcaact
     6901 cacccgggaa ccggtggatg agcgcaccga tttgtattcg ctgggcgcgg tcttttacga
     6961 ggcgctctcc gggcgcaaag ccttcgacgg cgacacgctg gcggtggtca tagacaagca
     7021 cctcaaccat gacaccctgc cactgcatgt tgtcgcccct catgtgccgc cctggctggg
     7081 ggcatgggtg ctgcggctga tggcgctgaa gcctgaggac aggccagtga acgcgcagca
     7141 ggccattgag gagtttcgcg cctgggagaa aatggcgtcc gctccgccca tgatgccctg
     7201 gatgccgatg ggttacggct actcgcctcc cgtccagcca ggcactggcc aggtctatcc
     7261 cggcactgcc ccggctcccg tgcagaccgg atatgtgcag ccggtgctgg aggtcatccc
     7321 aacggcgcgg cccgtcgtcg aagtggcggc gcgcaccagc gcgatcacgg gcgctgacac
     7381 agccaggcag gtcgtccgcc ccggaggcaa ccgcagcagc gcgctggcac gccccgcgca
     7441 aaaatccgcc tcggcggcca gcgggtcgcc gccggacaaa aagaaactct tcatcgccat
     7501 tggagccgga gtggctgtgc tgctgctggc gtttttcctt ttcggcggca gcagcgggga
     7561 agatgacagg ccggacaccg ccgccaagag cgcgccagtc cctccgccat ccgctggctc
     7621 ttcgaaaatt ctttttcaac tgcccgaatc ccgcgtcttt ccgcctttga gcgatgacat
     7681 cgtgatgcat tacatcgcca ccacgggcgt gtcctccggc cgcaaaggca gtgatggcaa
     7741 acccgcgctg gtcgccgtca acagccaggc atccgcatgg cacgacctcg ccgcgcgtgg
     7801 cgacgacaac gtcctggaaa caccggaccg caaacccgcg gccggcatcc ggcgcgtcac
     7861 atggaccact ggggacggtt tcagcggcat caaagacggg cgcaacgcgc tcaacttcac
     7921 ctccgaaggc ggcaaggacg ctgtgctggc ttttgaagca ccaggctcca aagcggcgca
     7981 catgccattc ggccaggcag cccgcaaggg agatgccggt ctcaccctgg cgctggtctt
     8041 tcaggcgcag gccgccgcgc taccgatgcg ggtgctcgcg ctggaggcgg acaaggacaa
     8101 cgccctgctc ctcggagtga acagccagag gcagatcacg gcggaatttc gcgccggggg
     8161 caggagtgag acgcttgccg ctcctcatct gaatgccacc cggccatgcg ccgtgctgat
     8221 cgcatggaat gccgacggca ccatcctgct gcgcgctcgc gaggcggcag ggcgcggtgt
     8281 cagcgtcgaa aagaaaggac attccgcgcc cggcaaagct ctcaaggggc tgcgcatcgg
     8341 cggaggcgaa cagggcgcat ccaccttcca ggggctgctt ggcgagttga tcgtttatgc
     8401 gtccgcattg aagcaagacc aggcccaact cgtcgccggc agccagattc cgggttttta
     8461 cttcaaggga accacacccc caaacctgcc caaagtcacg gccctgaccg agcggctctc
     8521 catcaaaaaa ccgcgcgtgg gcccgcgcac cgactgggct gtgaacacga gccacttgcc
     8581 ggaggacgcc tggctcgcca ttgatggcaa caccgccacc cgctggcgca cggacacgcc
     8641 ccagaaatcc gggcaatggt ttcaagtcac tctcaaggag gatcgtgaaa tctccggcct
     8701 ggcccttgat gtgtcctgct cgcctgacga gcatccacgc cgctatgagg tggagacatc
     8761 gcttgatggc aaatcctgga gccgggccgc cagcggggat aatgacaagc ctctgctgga
     8821 gatcgccttt ccccagccgg tgaaaacgcg gcatgtccgc gtcaagcaga ccggcggcgc
     8881 gccctccgcg cactggagca ttgcggagct ggaggtcttc gccaagtgag cggccgcccc
     8941 gcacaggttc aaagcggttg cggcaggctg gaatcaaggc tttcatgagt cccatgtcat
     9001 cacggcacga tcttcccatg aatcccccct tcgcgttccg tcctgccttt ggcgcgctcc
     9061 ttctcgcatt cgcggcgccc gctctggttt ccgcgcaagg cggaaaaagc ggcgtccagg
     9121 aattcgaccc gcgcgccttt cccggccagc tcgtggacga tgtggtggtg ccggtgccga
     9181 gcgaggtctt caccgtcctc gacaagctcg gctccccgaa ctggcgccag gaagtccgca
     9241 aagagaaacc gcccaagact ggcgaccgca cgcgcctggc gctgcttttc ggcctcaccg
     9301 tggccgaggg ttttgtggct gtccaggccg aggacaagga gtccgtcaag gacattggcc
     9361 gcgatgtcat agacctttcc accgcgctcg gcttgatcaa agccgtgcgc ccgcacgccc
     9421 aggccattct cgatgccgcc gacgccggcg actgggccac catccgcaag gagttcgaca
     9481 aaacccagaa gacagtgcgc gactccatgg agcaaatgaa ggacaacgac ctctcccaat
     9541 gcgtcagcat tggcggctgg ctgcgcggca ccgccagcgt cacctcggtg gtcaccaaaa
     9601 atttctccgc cgacagcgcc gagctgctga accagcccat gctcgtggag cacttcatca
     9661 gccgcatcga gcgcatgtcc aaggataaaa aggaccatga cgccatcacc gacatcgcca
     9721 agggcctgcg cggcatcctg ggccgcatgg aaggggcggt ggacggcttc acccgtgatg
     9781 ccgtgcgcga aatcggcaaa atctgcgaga ctttgctcgg cgaaatcaag gccgccaagt
     9841 aagcctgcac ccgcctcagg aaccccaagc caaggtcccg acatgaattt ccgccttcca
     9901 actctccgcc gcgcgtgcgc gttcatcacg ctcgcggttc ttctgttcca gagtccaagc
     9961 gcgcttgcca ccatagacga cgcccacagc ttcgccatgg aagccgccat cccttttgtc
    10021 aaaaagggct tcatcgtgcg cgaggactac tggaacggcg aggtcaaaag cggccagcgc
    10081 ctcatggtca aacaccagat gttcaagggc aacgaatacg ccttctggct aggctgcccc
    10141 gaggatgacg cggtttttga actgctcgtc ctagatgaaa agggaaatga gatcagcctc
    10201 gacttcaacg ccagccagta cttcgccagc gcccgcgtca acccgcccaa gaccggcacc
    10261 tacaccgtca ttttctccgt cagctccaaa cgcgccaaga gcctctactg ggctctggcg
    10321 tatgggtatc actgatccag cagatgcccg tcgagaccct cacttacgag accccgcagt
    10381 ttctgcaaaa gctcatcggg cacgatctcg gcggcctgcg agtcatcgcc gccggcacgg
    10441 agacgcagat cacctcgcgc gatgcctggg tgcgcattga aggcgcggac gcggccgctg
    10501 tgggcgcggc gcgcgaggtc ctcacccagc tcgaaaggct gcgccgccag ggcggcgaga
    10561 tcacatccgc gatgatcggc ctcgtcacgg aaagcgtgct caccggccat gacgatgccg
    10621 cccccgcatc ctccattcta actttgaaac tggccggctc cgccaaaaag ccgcccgtgc
    10681 ttgccaagac acgcggccag atcgcctatg ttcaggccat gcgcgaccac gagattgtct
    10741 ttggaattgg cccggccggg accggcaaga cctatctcgc catggcgcag ggactgcatt
    10801 atctgcggga gaagaccgtg cagcgcctcg tgctcacccg gcccgccgtc gaagctggcg
    10861 aggccctcgg ctttctgccg ggcgacctga aggagaaaat attcccctac ctgcggccgc
    10921 tttacgacgc gctctatgac atgctcgaac ccgacgaggc cgagcgcatg atcgagcgcg
    10981 gcgccatcga gatcgcccct ctcgcctaca tgcgcgggcg cacgctcaaa aacgccttca
    11041 tcattctcga cgaggcgcag aacaccacca ccgagcagat gctcatgttt ctcacccgcc
    11101 tcggcgaggg ggcgcgcgcc gttgtcaccg gggacccctc ccaggtggac ctgcgccgcc
    11161 atgtgcgctc cggcctgcgc gaggccgtgg aggtgctcca gggagtcgaa ggtgtgcgct
    11221 tcgtgcagtt cctgcccaag gacatcgtgc gcctgccggt ggttcagcgc atcatcgaag
    11281 cctacgacag gcgccgcgcg ggcggcgagt cgcgcggcgc tgcggacggc gcctgaatcc
    11341 cagccggacg cgccgcgccg cgtctcgacg actgtcgggt cccgtgatgc ggcatgctcg
    11401 actacgcttt cgcggccggc tccttcgact ggccctcctt ggcggcggcg cgggcggcgg
    11461 gtgtgtagta atagctggcg taattgtaat aggtgtactc cttcaagttc gaggaggagc
    11521 ggttgaggat gacaccgccg acattgacct gccgctcata caacgtgtcc agcgccttgc
    11581 cgctgagccg cgccatggtg gaggacatgc gcacgacgaa aagcaccgtg tcgagtttcg
    11641 gagcgaagct gcccgtgtca tccgccacca gcacaggcgc gctgtcgaag atcacataat
    11701 cgtagtcggc ggccatctcg cggaggaccg tctcggcggt ctttgagagc agcacttcgg
    11761 atgtctggtc aatgacctcg ccgcgcgcga gcagcttgag attcggtatg cccgtgtctt
    11821 gaagggcgtc acgccaggag acctggccgc gcagcacctc gctaaggccg ggggtgttcg
    11881 gcagcttgaa gagctcgctg acaccgccgc ggcgcagatc gcagtcggcg agcagcacgc
    11941 gggcgccgcc gagagccatg gtgatggcca gattggaagt gacggttgtc ttgccctcat
    12001 tgggcacggc acttgtgaca aggatggttt tgggcggatt cccctgccag tttttaaaca
    12061 ggatggagga tctgacattg cgaaacgcct ccgcgtatag gtggcgcgtg tcgttggcct
    12121 gcagcagcgc cacatccccg cgctgcttct gctccgggat ctggccgagc acagcctcgc
    12181 ctgggaacaa agcctggaat tccgcgaagg agttcatccg gtcgtccagc cggtcaaaaa
    12241 ggaggaggac gagcattccc ccggcgagtc ccgccagcag gccgccggtc agcggcacga
    12301 ggagtttttg ctcctcctga tagacctcgg aggcgggctc ctgcaccgcg acatagtccg
    12361 tctggatgtt ggtcatctgc tggaacttgg tgacggtttc agccatctgg tcgtgggcga
    12421 gtttcgcggc cttccactcc tcctccagac gctcgtgctc ggccagcttg gcgccgagtt
    12481 ccagcgcctc cttctccttg atggcgatct gttcgtcgag cacccgctga cgccgctcca
    12541 tgtcggaaat ctggccccgg aactccttga ggatcacgtc ctggaaatca ttcagcagcc
    12601 ttttcgccga ctcgatgcgc tcatccagcg cgacgacggc cggatgctgc tccttgaagg
    12661 acttgagcag gttcgcgcgc tcattgttca gtttgaagac ctcgttgcgg acttcgaggt
    12721 aattcctctc cgccaccgac atgctcaacg aggagcgccc gccttccact ttcggggtca
    12781 cccccgccgc ctcgcccccc tgcgcggcgg tcaccgcccc cggcgcggcc tggccgccca
    12841 gcaccgcgcg ctcgcggttg accatggcga tgtccacatc ctccagagcc agccggagct
    12901 cgttcagttc ggtgatgagc gcctcctttt ttatcctcag agactgtaga aacgcagccg
    12961 cgtcattgtt gccgttggtc agcagcacca cgttgttcgc ttttcgaaac gcctccagtt
    13021 tctcccctcg gtcctggagc tccctgccgc gcttgacgaa atcctcgatg aaggtgttga
    13081 gcgcgcgctc cagcccttgt tcccggatgt tctgccggaa tgcgatgaac tcgtccatca
    13141 acgcctgaag gaagattttg ggatactcct ggtcctggcc ggtggcgaac acgttgaaaa
    13201 tggcggaccc gcggttttgc accacctgga ttctgacctc ggaatccttc aagtcggggt
    13261 acaaggcgcg gatgcgggcc agcgcgcgct gcttcatttc cgggctctgc accgtgtcaa
    13321 ttatggtgcc gtaaaagtcc tgcaactgct ccacccatgt gaagtcccct tgtgtcgtca
    13381 tcctgccgcc ggcgaccagc ttggccacgg atttgtactc ctccggtctg ccggtaacga
    13441 tgactgccgc cacgcagacg ccgatgctgg cagtcagcaa gagaaaccac cagcgcctgc
    13501 ggaacagtat ctgataccgc tgcagcctgg ccgaggtctc atggatgcgg ttgagctggt
    13561 gggactgcgc ctggcctggc aggaccagcg gctgatccat ggagatggtc tgggtggtgt
    13621 tcacaatggg aaatgagggg cggaatgatg cgcggcatca atctccgccg caagcagggg
    13681 ggatggaaac tgccacacaa aaagaaaacc gcctccaaaa aagactccgc gcgccgctgg
    13741 ctcagatgtt gatctttttc tcgtcaacga tgatgacatc attgttccgg atatagacat
    13801 cgtattcgag gttccccttc ctcatgatgg tgtcgaggtt gatgatgatg gtcttgttcc
    13861 cctggggcgt ctttctgacc agcttgacct tctcatggtt ggcgaactgg gcaaacccgc
    13921 ccgcgcgcag aatggcctgg ctgatggtga tgtcctcgtc cagcggcagc tcgtatttgc
    13981 cctgccgcag cacctgcccg taaacggtga agaaatctat ttcggagctg ctgtagcgca
    14041 ggcgggcgtt tgggtcgtcc tggcgctgcc ggtcaattgc cacgatcacg gtcgccttgt
    14101 tgtagtagct agcctcaagg cggcgcttga cctcgtaggc cagttgcttg gaggtgcggc
    14161 ccgccgcttt gacaagcccg atgtggggcg cgtaaacctc accagtgaca ccgaccgtga
    14221 gctggcgcgg ctcggtgcgg tcctcgacga tgcgcatgct aatcgtgtct ccgggttcga
    14281 tggggcgcga gtcatccagc acatccatcg aattgatgat ggcggcggag cgggcgatgt
    14341 ctcccgtctg caccggcgcg ggcgaggtct gcctgcggtc ctcaaactcg cgcacaccgg
    14401 gatcttgggc ggtgagaaag gcgggcagca gcgcggaaaa agcgataagt ggtagggtgc
    14461 gcatggtttc ggatcagaag ttgtagttaa agcccatgat gagcctgttt tgcgtgaagt
    14521 cctggtcatt gaaccggccg ggaccggaaa gcatgtccgt caggtagtaa cggtagccga
    14581 agatcaggct ggccttgtgg ttgagggcgt aagtgagatc cacattccag gcatgctggt
    14641 cgaaagttcc aaaattcgcc gcgctgccga ggctgccgag ggcgttggag ctgcccatgc
    14701 ggtcggtgtc agtccgtccg tagtggtagc cgacacctgc cgttagcttc gaagtgaccc
    14761 ggtgcgagac gtgaatatcc atgccatact gcagagtctc ctgcgcgaag accccgccgg
    14821 acatctcagc gtcctccaca taggcgctaa gggcgatgcg gctgcccttc caggcgatga
    14881 aggagatgcc gtagttcaaa taatcggcca cgacatagtt cgaggtgaca ttgagggccg
    14941 cctcccggcc aaaggtcagg ctgtggctga cgcgcgagtt caactggttg ctgagcgtga
    15001 cgctgtagta gaagtcgctc aaatcagagg tgtcgccgga agtgagcgga gttgtgccaa
    15061 ggctcttgaa gcccacctcg tcgaactcga aattctgcca gccgccagcc acgcggatgt
    15121 aggtgctttt ggccaccggg agcaccacaa aggcgccaat gttggaaagc acgccgtcgg
    15181 cgttgaactc ctggtcatac tgcatgtccg tgacgccgcc ctccatgccc agaacccagg
    15241 ttccggtggg actgaaggtc agcgaggcgt ggatcgagtc cgtggtgcgg ctgaaattgt
    15301 cctcatcagt gacgcccacc gtgcctgtct gcccgcctcc gagcgcctgc gaatccgagt
    15361 gcatgaggtt caccgacagt gaccatgccg agttgatgcg ccagttggcg gccacaccca
    15421 catcattttg gaagaccccg aagacctggt tgcgggtcag ggcgaagtca ttgcgcaccg
    15481 ccggacgcac cgaaatgcgg tcatagacgg tgaaatagac cggccctgcc ttgatgtcga
    15541 aggcgatggt gcttcctggg aggacgttga cgatgaaatt gccgtcaccg tagggggaga
    15601 gttcgggatg attccaataa ttgtcaaagc cgagcgcggt ggagatggag agccggttgt
    15661 tctgcgtcac ctggtagttg gcgtcaatgt tcagcaaagt ggaggtgatc acatccgaga
    15721 ccgggtccac acctgagcgg ttgacattgt cgttgtattc gatgccctgg taaaaaccga
    15781 agcctacatt gaccggcccc aggcgcacat tcctgcgccc ggaaaggaag gggtccgaga
    15841 tgtaggtcgg ggcgaagaaa cccgtcggcg aggtgtactg ccccgcgtag ttggtgtagt
    15901 cccccgccgt gcgggccgag aaggcgttgc tgccgcttct gatggatggc cggctcacct
    15961 cgcgcgccga ctggtagtcc gtgctgcgct gccccatttc atttccggtg gcgcggtaaa
    16021 tcccgctgtc atagtcgtac accacattgc catcccgggc gccagatgcg ctggccgcgc
    16081 tctccgcccg ctggcgcgcg cgcagatcgt caaaaaacat gccggtctgg ctcggcgaca
    16141 ctccgtactg cgcctgaact tcgacgcagg ttaaggtcaa aacagcggct gccgccatca
    16201 atccaaaaaa tggacgacga ctcttggggg gattctgggt catggtgggg tttgcgagat
    16261 agtgtgaata tccggtcaaa aaatgacaat ttcggattta aacgaacccg accgggattg
    16321 tccattcaaa acacggcctt tagcagattc taaagaatga agaaaacctg aggccggaag
    16381 ccccggcaag acccggagca actttcattt agcgcctgct gaagcgtttt ggtgaagatt
    16441 tgaggcgcgc gcatggccga aaaaccattc gcgcacccgg catcgtccgc tatcctcgcc
    16501 gcccttatgc tccaagctgg aatcgtcggc ctgcccaatg tgggcaaatc caccctcttc
    16561 aacgctgtca cacgcacccg caaggcggag gcggccaact accccttttg caccatcgaa
    16621 cccaaccagg gagtcgtcat cgtgccggac gagcgcctgg ccgtgctctc gaagctcagc
    16681 ggctcgcaaa agctggtgcc ggcggccatt gagtttgtgg acatcgccgg cctcgtcaaa
    16741 ggcgccagcc agggcgaggg cctcggcaat aaattcctca gccacatccg cgaggtggac
    16801 gccatcgtcc atgtggtgcg ctgcttcgag agcggcgaca ttacccatgt ggacggcagc
    16861 gtggacccgg tccgcgacat tgaggtcatt aacaccgagc tcatcctcgc cgacctcgag
    16921 gcagtcaccc ggcgcaagga gcgctgcgaa aaaaaagcca agagcggcga caaggagtcc
    16981 aaagccgaac tcgccctttg cgagaagctc ctgcctcacc tcgacgccat gaagcccgcg
    17041 ctcaccgccg atctcagtga tgacgagcgc aagctcatga aatccttttt cctcctcagc
    17101 ggcaaaccct gcatttacgc ctgcaatgtg gccgaggccg acctcgcctc cgcctccaag
    17161 gagccggaca agcacccgct cgtctcaaag gtgcgcgaat atgccagcca cgcccacgcc
    17221 gccagcgcct gcgtggtcag cgccgccatc gaaaacgaac ttgtggatct tcccgatgag
    17281 gacgccagcg cctatttagc cgacctcggc gtggaagaca gcggcgtgaa ccagctcatc
    17341 aaaagcgtgt actccctgct cggcctccag acttacctga ccacgggcga gaaggagacc
    17401 cgggcctgga caatcaccaa aggcatgaag gctccgcagg ccgccggcgt cattcatggc
    17461 gatttcgagc gcggcttcat tgccgcccag atcgtgagtt ttgaagacct cgtggcctgc
    17521 ggctccgaag ccaaagcccg cgaggccggc aagctgcgca tcgaaggcaa ggaatatgtg
    17581 atgcgcgacg gcgacgtggt ggagtggcgc ttcaacgtgt gatccgggcg cgccttgcca
    17641 gatcaaaaaa agcccgtgtc ccttccggga acacgggctc gtgaaatcag cctccaagga
    17701 tcagtcggcg tccgcttcct cgctttcggg agactcggca ccctcggcgg cgctcacatt
    17761 gagtttgacc acgcaggaga cttcggcgtg cagtttgacg ggcacttcga aggcgccggt
    17821 gcttttgatg ggcttgtcca gctcgatctg gtggcggtcc agttcgatct tggcgctttc
    17881 agcgatcgcc ctggcaatgt caatggtggt gatggagccg aaggccttgc cgccctgtcc
    17941 ggtctggaga gtgagcttga gcttcagttt cttgagcttg gcggcgactt tttcagcctc
    18001 ttgcaactcc ttcgcctcgc gctccgcgcg gaccgctttg aggcggttca aatgacggag
    18061 gttgccgggg gtggcatcgt aggccttgcc ctgggggacg aggaaattgc gggcgaagcc
    18121 gcgcttcacc ttcaccacgt ccgcttctgc gccgaggcct ttgatgtgct ctttgaggat
    18181 gacttgtgcg tgtgccataa atgggattcc tttgccagga agggggcgcg aggctacacg
    18241 gcacgccccg cctgtcaaat gctgccttgc ggtctttttt gtcatctcca tgctcacaaa
    18301 gcccggtttc ggccctgaca gtcgcgcgtg ccacagcttt catgaatgcc agacatcctg
    18361 gaggcctttt tcaccttgca ccccgccgcg ctccgcgcca tctcagatga acctgcccgt
    18421 caccgccttg gctgccaccg actccgaccg aacactcgtc gccatacctg cccgctgggg
    18481 atccacacgc ttcccaggca agccgctcca cctcatcgcc ggcaagccgc ttgtgcagca
    18541 tgtctgggag cgctgtcagc agtgccggaa tgtggacgaa gtgatcatcg ccacggatga
    18601 cgcgcgcatt gccgaggcgg cggcatcctt tggcgcgaaa tgcgcgctca ccgcgccgga
    18661 ccaccccagc ggcaccgacc gcatcgcgga agctgcgcgc gccttcccgg accaccgcgt
    18721 catcatcaat gtgcaggggg acgagccatt gatctccccc gcgctcatag acgagctggc
    18781 caccgtgctg gtgaacgagc ctcaagtgcg catgatcacc gccgccgcgc ccgttctcga
    18841 cccggcgcac atcaccgatc cgaacatcgt gaaggtggtc ttcgacacgc atggagactc
    18901 gctttatttc tcccgctcgc cgctgccctt cattcgcgac gcctccgccg gcgcccggca
    18961 ttaccgccac ctgggcatct acggcttcca gcgcggtttt ttgtttcaat tcgttgcctg
    19021 gccgccctcc aggctggagc gcgccgaagc cctcgaacag cttcgcgccc tggaaaacgg
    19081 cgtccgcctg cgcgtcgtcc tcaccgatga aatctccccc ggcgtggaca cgcccgagca
    19141 ggctgcggcg gtcgagaggc agctcgcctc agccgtgctc tgattccgcg ttcaagactc
    19201 catcccccac tcattatgaa atacatcttc gtcaccggcg gcgtcgtttc ctccctcggc
    19261 aaaggactcg ccgcgtccgc tctgggcacc ctgctggaac tgcgcggcct ccgcgtgatc
    19321 atgcagaagt tcgacccgta tctgaacatt gaccccggca ccatgaaccc ctacgagcat
    19381 ggcgaagtct atgtgctcga cgacggcgcc gagaccgatc tcgacctggg gcattacgag
    19441 cgtttcaccc acaccaacct ctcccggctc aacaacctca ccagcggcca ggtctatatg
    19501 agcgtgctcg agaaagagcg cgcgggagcc tacgagggcc gcaccgtgca ggtcatcccg
    19561 cacgtcacca acgaaatcaa ggaccgcatc cgcgaggtcg ccaaaaacat gccgggtgac
    19621 atcatcatca ccgagatcgg cggcacggtg ggcgacatcg agggcctgcc gttcctggag
    19681 gccatccgcc agttcgccat cgaggcgggc cagggcaatg tgctcttcat ccacgccacg
    19741 ctcgtgccct atatcaaggc cgcgcaggag ctcaagacca agcccaccca gcagtcgctg
    19801 gccaagctgc gcgagatcgg catcgccccg cagatcatcc tctgccgcac cgagcacccg
    19861 ctggacctgg atgtgcgcga aaaaatctcc cttttcggaa acgtgccgat cgaggatgtg
    19921 atcgaggtgc gggacgtgaa gcacagcatt tacgaggtgc cgctcaagct gcacgagcag
    19981 cgttttgacg accgtgtctg caaggtgctg catatcgagt ccccccagcc cgacctctcc
    20041 aagtggcggc actttgtcca gcgcatcatc caccccacgc accacgtccg cataggtgtt
    20101 gtcggaaagt acatcgagct tcaagacgcc tacaaaagca tctacgagtc cctcacccac
    20161 gccggcgccg ccagcgattg caaggtggac atcgtgaagg tggatgccga ggccattgag
    20221 aaaggcggcc cggaccttta tctcaacgga ctccagggca tcctgattcc cggcggtttc
    20281 ggcgaccggg gcacggaggg caagatcgcc gccgccggct acgcgcgcca gcatggcata
    20341 ccgtttttcg gcatttgcct tggcatgcag atcgccgtca tcgagttcgc gcgcaatgtt
    20401 tgcggactgg cgcaggcgag cagcaccgag tttgacaagg gcacgccgca ccccgtcatc
    20461 agcatgatgg aggagcagaa aaaaatccgc catctgggcg gcacgatgcg cctgggcaac
    20521 tgggtcacgc ggctcgtgcc gggcagcaag gcgcacgcgc tgtatcaaag cgacaccatc
    20581 gaggagcggc accgccaccg ttacgaggtc aatgaggact acaaggacac cctcgaatca
    20641 cgcggcatga tcatcagcgg cgtctcagcc aagggcgatc tcgccgaaat ggtggagctg
    20701 cccgcccatc cctattttgt ggcctgccag ttccatccgg aattcctcag caagccgaac
    20761 catccgcatc cgctttttca cggcttcgtc caggccgcca tgaagcaccg catcgtgcct
    20821 gagccgcact gctgaggcaa aggcgctccg ggcctgccca gcgagcgctc aagcggcagg
    20881 caactggtcg aggcatattt gggaccacgt ctccaattcg tcgcgcctgg agcgcagttc
    20941 gtcgagagtc atgaagcgcg gatcgtgaat ggcgtcctcg ttggaggcca catcgtcatt
    21001 gtccaggtca aagagatgca ccacgccgag atggacgctg cccacctcgc tgctgtcgtc
    21061 attaatgagg ccgatcacgc gctggctgcg ggcgccggtg agcacgagct cctcggccag
    21121 ctcgcgctcc aggctgttga aatacattgt catgcccatg ccgtcgcagg actggtccac
    21181 agggttgata tgaccgccga caccgaggct gcgccgtgcc acaagacgtt tctccccgga
    21241 gctgccgccg cgctggtagc agaggtagcg cccggcgtgg tggaagatgg aataagggat
    21301 caactgcttg tgcgaggggt ccgtctcggc ggcgggacgc tccatgaaaa aattcgcgcc
    21361 gggcgcgagc atggcctgca aaaatcggtc cgcgtccggc tggaagccct gaaaagtgcc
    21421 gaggctgtcg aagagtgcgc gggggatgac gaggacgtgt tccatgacgg cgaccagatt
    21481 gggcggctga cgtacaaggt caacgacgga ggctcatggc attgacggca ctcaagtttc
    21541 agtggacaag actcgggcgc ggcgttttgc gtccgtgcat gactccagac tccccagtgc
    21601 tgcgcgtgaa cgaaggcgat gacctcatcg tggcgctccg agacctcaaa gccggcagcg
    21661 tggtgacgct cggcggcgaa tgctggacgc tgcgcgagga tgtgcccgcg aaacacaaat
    21721 tcgccgcgcg cgactttgcg gcgggcgagc gggcgcgcat gtatggcgtg gtggtgggaa
    21781 agaccacgca gacggtgccg gccggcgctc tgctgcgcac gagtaatctg gcgcacgacg
    21841 cggcggcctt tgccggcaag cagcgggatg cggcctggac ggcgccggac gtgtccaagt
    21901 ggcggggtcg ggtattccag gggtacagac gcggcgccgg aatagccggc accgcgaacc
    21961 actggctggt catcccgttg gtgttttgcg agaaccgcaa cttagcggtc atgcgcgagg
    22021 cattgacccg ggcgctgggc tacgcgcatg gcagcgctta cgagcgctac gccgcgcatc
    22081 tggcggaggc gcgtcatcgg ggcgtgcccg cgacgctcgg cctcgatgac tgcttcggcg
    22141 cggaatccac gccgctcttt gccaatgtgg acggggtgaa atttctcgaa cacggcctgg
    22201 gctgcggagg cacgcgccag gatgcggagg cgctctgccg tctgctggcg ggttacatcg
    22261 cgcatccgaa tgtggcaggc gccacggtgc tcagcctagg ctgccagcac gcccaggcga
    22321 gcctgctgga gtcggccctg gcagagcttc atccaggcca tgaccgtccg ctgcaaatct
    22381 tcgagcagca aaaaagcgcc tcggagcgcg acatgctcgg ccgggccatc cagtcggtga
    22441 tttccggcgt ggcgaaagcc tcggagcaaa cacgggaatc ctgccaggtc tcggatctcg
    22501 tgctgggtgt ggaatgcggg ggatccgacg gcttttcggg catctccgcc aatccagtca
    22561 tcggccgcgc cgccgatctg ctggcggcct gcggcggcac gccggtgctc gcggaattcc
    22621 cggaactgtg cggcgtggaa caggaactct gcgaccgctg cgccagccgg gaactggcgg
    22681 agaaattcac ccgcctcatg cgcgcttacg aagccgcagc gcgcgcctgc ggatcaggtt
    22741 tcgaagcgaa tccatcgcca ggaaacatac gggacggcct catcaccgac gccatgaaat
    22801 ccgccggagc cgccaaaaaa ggcggtacgg cacccatcgt ggatgtgctc gactacggcg
    22861 agccggtgcg caggcggggc ggcttgaatc tttgctgcac accaggaaac gatgtcgagt
    22921 ccaccacggc tctggccgga gcgggctgca atctcatcgt cttctccacc ggccttggca
    22981 cacccacggg caatcccgtc tgcccgactc tgaaagtcgc caccaacacc ggactgatgc
    23041 gcaaaatgcc ggacattttg gatttcgacg cgggtcccgt catcacaggt gaaagcaccc
    23101 tggaagatct cggtgagaga cttttcgaag catgtctggc ggtggcgtcg ggaactttgc
    23161 agccgaaagc tgtcgccctt gggcaagacg acttcctgcc ctggaagcgc ggtgtgtcct
    23221 tgtaaatcca caggattgaa ttgcattctt atgaacagtt aatgaaattt atttaattta
    23281 tctaaatatc tagttggctt taaacgtttg agagttactc gttgcctaac ctcttatgac
    23341 acgtcctgcc gaacccttgc tctatctggc ggagcctgct cctgcggtcc gcaagtctcc
    23401 cggggtggtg aaaatttccc ttttcgacca ctttgttcac ttcaccattt ttcttttcct
    23461 ggtgggcggc tgcgccctgc tgctggatca agcttccgcc cgaacgacgg gacatgaggc
    23521 ccaggccggg cactggttcg cccacggctg ggccgccctg tccacattcg ggtcactttt
    23581 tggcaaatac accgccctgt ttggagccag cctggtcagc acggcgctga tggtggccat
    23641 ggtccagtca ctgaggctgc tggatgtcat gcattgccgc aacgtcgcgg cggcgctccg
    23701 tgagaaaccc ggcggtggaa cccaggtcac acgcgccatg gcgcgcgacg gcggatatta
    23761 catgctcgca ccggtctttg agtgaccaga gatggtaccc agggagggat tcgaacccac
    23821 gaccaattgg ttaagagcca actgctctac cactgagcta cctgggcaaa aattgacttc
    23881 catcagatgg tacgcgggga ggggatcgaa ccctcgacca attggttaaa agccaactgc
    23941 tctaccgctg agctacccgc gcggatgggg cgcattacct agcgcggaag gtcctccacg
    24001 caagtttcat tttccacgcg cgggcatttt tcatcccgct tcgcgaagcc tgctgcgggc
    24061 tggattccag ccgtggcggc gcccgggttg caagcgacgg cgcgccgcct agagtcggag
    24121 tcatgaacac atggactccg cccaacccgg tgaacctggc cgacctcggc ttcatggacg
    24181 cccgctcgaa actcatcgac cttgccgcct ttcttgaccg ggtgcagcgc gccgggcagg
    24241 atgacgactt ccgcgtccag gcgctgaaga aagccgccgg attgctttcc ctcgaccagc
    24301 cgacccgtgc ccgcgaggtg ctgctgagct tcagcgatcc cggcgccgaa cccgtggaaa
    24361 aggccgccat gcagggcgcg ctgggagcct tcaaagtctg acacgtcatg aactacatcg
    24421 aaccccacgg acacatggtc agccgcacca cggacgacta tgagcgcatg gcgctggccg
    24481 gctgccgcgc gatctgcgaa cccgcgttct gggccggctt cgaccgcgcg tcggcgcagg
    24541 gcttttacga ctacttccgc cagatcaccg agtatgagcc gaagcgcgcg gcgaagttcg
    24601 gcgtcgccca ttactgctgg ctgtgcatca atccaaagga ggcggaggat ttgaagctgg
    24661 cggaggaggt catcgggatc atcccggagt tcctcggcaa gccgggcgtg ctcggcatcg
    24721 gcgagatcgg cctgaacaaa aacagccgca acgagctgcg catcttcgaa cgccatcttc
    24781 aggtggccca ggagcacggc cagctcgtgc tcatccacac cccgcacctt gaggacaagc
    24841 tcaagggcac ccgcctgatc gtggaagcgc tgaaaaactt tcccgccatc cggccggagc
    24901 gcgtcatcat tgaccatgtg gaggagcata ccattgacca tgtgctcgac cacggcttct
    24961 gggccggcat cactctttac ccggagagca aatgcacgcc gcaccgcgcc attgacatgc
    25021 tggagcagcg caaagccgcg cgcatctgga tgaacagcgc ctgcgactgg ggtgtgagcg
    25081 acccgctggc cgtgccgaag accatgcagg ccatgcgcca gcgcggctgg agccgggaga
    25141 tgatccagcg tgtggcctgg gagaacccgg tggctttcat gaggcagtgc cccaagtttc
    25201 aacctcccgc atgaggctgc ccccggcctc tcccgcccat gacccgcgcc ctctcctacc
    25261 gcgacttctc catcggtctg gcgctgcttg gcgtcaccgc gttcttcgcc gttgtcgagc
    25321 cgaagtttgt ggactcgcgg aatctctccc tgctcatgac cgagctgtcc atcaccgcca
    25381 ctctggcgat gggcatgctg ctcgtcatcc tgccggggca tattgacctc tctgtcggca
    25441 gcgggctggc cctgctcagc ggcatcgcca ccgtgctgac cgcgcgcgct ggactgccct
    25501 cccccgtggc gctgggcatc gcctttgccg ccggcctgct ggtatggtgg gccaaaggct
    25561 ggctgatcgt gcgcgagcgc atccccgcct tcatcgtcac actggccggc ctgctcatgt
    25621 ttcgcggcct gttctggctg gtcattcaga atcaaaccat ccccgtcgtg ccgggcggtc
    25681 aaacaaatct ctactccctg ctcagcacct ggtatctgcc gccgcctctc ggctgggcgt
    25741 ttgccgccct ggtcatcgcc gcgctcggct tctgcgcctg gcgcgggcgc agaagc
//
LOCUS       DAOMYJ010000052        25695 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Prosthecobacter sp. isolate AMR_MDS_5629
            NODE_691_length_25695_cov_20.760842, whole genome shotgun sequence.
ACCESSION   DAOMYJ010000052 DAOMYJ010000000
VERSION     DAOMYJ010000052.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867850
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prosthecobacter sp. (wastewater metagenome)
  ORGANISM  Prosthecobacter sp.
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Verrucomicrobiaceae; Prosthecobacter.
REFERENCE   1  (bases 1 to 25695)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 25695)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.30
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:34:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,742
            CDSs (total)                      :: 4,684
            Genes (coding)                    :: 4,676
            CDSs (with protein)               :: 4,676
            Genes (RNA)                       :: 58
            tRNAs                             :: 55
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25695
                     /organism="Prosthecobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_691_length_25695_cov_20.760842"
                     /isolate="AMR_MDS_5629"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1965333"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..4470)
                     /locus_tag="PLP58_07570"
     CDS             complement(<1..4470)
                     /locus_tag="PLP58_07570"
                     /inference="COORDINATES: protein motif:HMM:NF041518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choice-of-anchor Q domain-containing protein"
                     /protein_id="HRJ08408.1"
                     /translation="MKTRLIVAIASVVLSPALHAATLTVTTNADSGTGSLRQVLADAA
                     AMAGPDTIVFDPLLAGQTISLTTSSNGSAITINDAGGVTIDATGVPGIVIDDGTATSY
                     GLFFVTPDSVGTFRGLTFANGGGANFSTGGGAIGNGGTLKLERCTFSGNSTTGGGFAD
                     GGAIASVGTLTLTHCTLTGNSAGDTGGAIDFAGTLTLTHCTLSNNTAGNTGGAIRNAG
                     MLTLNNCIVAGNTAPTNANISGTIGSGSNNLTSGDPKLAALADNGGPVETMALLPGSP
                     ALDAGVAMPGIDLDQRGFQRSRDGDATPGALPDIGAYEAQAAPTVSNPNPIVVTTAAD
                     EADPNGTLGVGISLREALRDGGGLGSIIFDAALSGQTITLSSSLGDLVIEKSVSIDAS
                     SLAGGVTITRDGTGVFRLFTVLPGKTANLAGLTLTGGEGGGQNLTTTGGAFQNAGTLT
                     LTSCTLSGNSATDGGAIFNQGTLTLVRCTLSGNQTNTAAGLHRGGAIHHASGTLSLNN
                     CMLEQNSCVGDGGAIYLFTGTATLANCTLSANSVTDGNGGAILSAGVLTLTDCTLSNN
                     RAFWSNGFADSGVGGAIANTGGTLTLTGCTFAGNATAKTAAELSTPIFDGSARGGAIY
                     NFGTVPALTNCTFVGNSSTGDGGAICNFGPLTITHGTFAGNFINNGDPAANGFAGNGG
                     AISVLGGTLSLGYSIVAGNASVSGPDLHKPASGGGTITPVGTNLIGKNNTVMTEFPQG
                     PLVGIEASPVPANLDPLGDYGGPTQTMRPQPGSLAIDAATGSSTTTDQRGNARPTDGD
                     GNGSVVADLGAAERGTLLLPVVSTTADSGAGSLRQAIADAAAVPDTTITFAAGFTGPI
                     VLASEIVVPMNSVITVDGSNVTAGVVLSGGGTSRIFQVNTGGNLTLKAVTLTGGNGTG
                     TSFSGYGGAISNSGTLTLTQCTLSGNSANIVGGAISNSGTLTLTQCTLSGNSANIVGG
                     AIQNQSTLTLTQCTLSGNSSNDGGGAIYQYGGTLTLTNTIVAGNTAPTGPDIYSLLSL
                     IGDSTITPTGKNLIGNLAGSGLNANGTTVITGAANLAPLGHYGGRTPTMLPLAGSPAI
                     DAGDNAVIPAGVTTDQRGSFLPFNGTVDIGAVERGYVVTNTNGSGAGSLRQAVNNTGT
                     GGMVINFDPALSGETINLSSGITIDRDLEINAGGLANGVTVNGGSQNFRLFQVGAVRT
                     VAMHNLKLINGGGTAFNGFGGAILNDGNLTLTRCALSGNQVMAGNSGGAISSGTGSTL
                     SLTDCALSGNEADFGGAIETVSAATLNRCTLNGNTARAGNGGAIDGGTSANIALTQCT
                     VASNTATALGGGVAVFDGSLSLIHCTLGLNSAANGGGASVKGSFSVANTIFAGNAATI
                     AGPELFENGAFTSLNFTGRIFLQQLGGNGFFASGNVLTGIPELSLPGNYGGATQTIVP
                     LPGSPVLDQAGSTANVGAALNVAFTTDQRGFARAADGDGNGSALADLGAAERCH"
     gene            complement(4513..4962)
                     /locus_tag="PLP58_07575"
     CDS             complement(4513..4962)
                     /locus_tag="PLP58_07575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ08409.1"
                     /translation="MKTLHTMSFAFLALLLGSVLSTCGGQPAIVGGPCTYDDHPGSAT
                     ITRIEKTEASKQQSETTGYEGFEVRFTFKPDAPLKDDLGARAATWEHSFQIGSGTYPG
                     PEYLAKYKLEAGKTFRGTMSVIQSGTCTPVIVKLEGVDPADFFEGKK"
     gene            complement(5001..5348)
                     /locus_tag="PLP58_07580"
     CDS             complement(5001..5348)
                     /locus_tag="PLP58_07580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ08410.1"
                     /translation="MKHYLACLLARVACAMLFLGTTTVVRADLPQQRKALDELQAAKK
                     AADPMPLLESAKNRITKANKGNKGGDKEDVIAKINEAIEQLEAGNRSKMEQKINAAIA
                     NLHQGKDKSKRPK"
     gene            complement(5472..7076)
                     /locus_tag="PLP58_07585"
     CDS             complement(5472..7076)
                     /locus_tag="PLP58_07585"
                     /inference="COORDINATES: protein motif:HMM:NF019334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HRJ08411.1"
                     /translation="MSDSSASLHTCPQCGAALPAEATEGLCPRCLMAQAMETSQTGTA
                     PPAAASEIPLPEELTGLLPADTYRVDYLIRRGGMGAVYRGAQLRLERPVAIKIMRREQ
                     GRDHGFEQRFQREALAMAKLNHPNIVSVIDYGEAGPDYLYIVMELVEGADLMDVIRSG
                     RMTQEMALTLLPQICDALQFAHDHGIIHRDVKPSNVMLTRDGRIKIADFGLAKRHDVE
                     SSFHTRSGTGMGTPDYAAPEQFIAAAQVDHRADIYALGVMIYQMLTGQLPRGAWKPPT
                     QHTPCDPHWDDIVSHAMQTDPKDRYQAASEVKSDVSRIGPGKDTGIEGSADTPVRSQS
                     SGGAAATNEEAGKSARAPLQSRTWLVPVILASLIVISAGVFFASRPVGGGPIRTETVK
                     SAGVHTFAGHRYRFVPGAMTWDEAKAKAESMGGHLVTITSREENAWIDATFGTKLDGA
                     PRSASIWLGASAEQKGQPFRWVTGEPFTFTDWGTDEPNYATTTGKPVEGPFAISIKSH
                     AQTLFWFDDPTSRANPSVGFIVEWDS"
     gene            complement(7240..7974)
                     /locus_tag="PLP58_07590"
     CDS             complement(7240..7974)
                     /locus_tag="PLP58_07590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009960433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HRJ08412.1"
                     /translation="MSQQPHPPSDAASFHTTRWTRVGLAKTESEDGRRALADLCDAYY
                     EPVVAFLRWELRDADAAREMSHAFFAEMLAGGTIAAAERERGRFRTYLLGAVKHFLAN
                     QREAARRMKRGGGAAHVSLNEDASGVCEIPDSRQMTPDAAFDREWALTTLARAMDAVK
                     QECVKEGREAFFQRVKPWLTGDATHGDQGAAAEACGMSAAALKMAISRLRRRFRECVK
                     EEIAGTLDDAAMVEAEMRELFAALAG"
     gene            complement(8120..8914)
                     /locus_tag="PLP58_07595"
     CDS             complement(8120..8914)
                     /locus_tag="PLP58_07595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009960534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HRJ08413.1"
                     /translation="MNRLENKVILITGSVTGIGKAIAKRCVAEGAKVLIHGLETDLGQ
                     ETLAELGADKAVLVLKDLAEEDAPQLLVEKAVSAFGKLDAVVNNAAQVGLGNIHDTDA
                     KWFRRMLEINCVVPYLLIRAALPHLREARGSVINIGSVNAWSGEPNLMPYSVSKGALM
                     TLTRNLGDTLMREDGVRVNQINPGWVLTENEAKRKRSHGLKDDWYADLPKVYAPAGRI
                     LWPEEIAACAAWLLADECGPISGQVFDLEQHPFIGRNPPKDASTIP"
     gene            complement(9049..9690)
                     /locus_tag="PLP58_07600"
     CDS             complement(9049..9690)
                     /locus_tag="PLP58_07600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ08414.1"
                     /translation="MRRTIILLCLLLCLIAFWLMRTAWEPKETKKPVSAPQVASTSSS
                     SISTPTPPKAEKAPAVRKPLKILSKGKVRLKPGESAVLGYWEIAPGMNGMAIVTPETT
                     PEGHVKMTAKLLHLSDAAVADAEAHDLFPDLFDFENYSAIGPERLRKLQSSLQNTRGA
                     DMLSMPTVATRPGQQVSISIGNSNETTLSLGLQADPVAEYGGYDLSLDLQRQE"
     gene            complement(9740..11185)
                     /locus_tag="PLP58_07605"
     CDS             complement(9740..11185)
                     /locus_tag="PLP58_07605"
                     /inference="COORDINATES: protein motif:HMM:NF018178.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1080 domain-containing protein"
                     /protein_id="HRJ08415.1"
                     /translation="MKTLLLLSLLTTSALAADLKPNAALTFDFPELPNTLAGMVSGTA
                     KVARLGAILPSNYATTAKHPLFVYIKGGSGGPEDGGAMARDIVGAEDFIAVQVPLFKK
                     NQPKGKTLPVTVEDFAVVSAAYRTMLEKLAAEVPNIDWERSVIGGHSNGAHTLSVLLA
                     GRDEFITQHFHAFWLHEGGFQLMPAAMPIKDGRFLLLVADDAMTGGSEFRQLMLDQTS
                     LLERQAKLMKLDFKSVIMRGYGHDVPPEYMRNVRQFARGEPLEDITASEKAAAAKLTL
                     PLRTHPDSSAWNDLLVSDLTNMKVPGAVWSHRDGLLTATEDQNIWTKATHGDCVLDFE
                     FKFEPGANSGVFLYNSDETNWMPASIEIQICDDRARQWQEKPASWRCGAFFGHQPATK
                     STVKPAGEWNRMTLTAQGPKITVVLNGDVVNEIDLTQWKDSKLNPDASEMPKWLQGRP
                     WSEMPAKGRIGFQGRHAGAGIEFRKIKWLRL"
     gene            complement(11272..12858)
                     /locus_tag="PLP58_07610"
     CDS             complement(11272..12858)
                     /locus_tag="PLP58_07610"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF015692.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribosylglycohydrolase family protein"
                     /protein_id="HRJ08416.1"
                     /translation="MTKTSLPTIALILLTCASSLAAEPLKLTRADYEDRVNAIWHGQL
                     IAVLLTLPFEHHVAAAEPIRAFPKPYTAAFVDDDWYYEMCAVRAFEKHGIGLTLEQLG
                     EQWRENACGSYGSSMVTRANLEKGLSAREAGHPRYNRLWWTIGPVFSADLYGALAPGM
                     PNEAGRLARELCAINGYGEALDGAVLYAGAISLGFAESDVREVLRKAVRLIDPSSPYR
                     QCVESVIHMAENGQSFEAIAAAVEDRWRVEYPASNNAVPNGGLAAACLWFGNGDFWTT
                     IDLACRAADFTDTDNSAATAISIIAAMKGMKALPAELVAQLNDRITGEKLGRVTVTPA
                     VDEKISTLAKRTAAIGEKIITAKGGKISGDEIHILPEAVVTQAAQRFQLADLTQWWNP
                     EWTLERAGFGGDGVGAMPGIRGYTALDGDTLITYPRDEARSLLLRRKLKLGDAPSLEL
                     QVAADPGKAWELNVYADNKRVLARTIEGSGTEVRFETIKIDLGDFAGRETTLRLYQRV
                     LLPGQLKVPGVAYWKSPVVR"
     gene            complement(12933..13124)
                     /locus_tag="PLP58_07615"
     CDS             complement(12933..13124)
                     /locus_tag="PLP58_07615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ08417.1"
                     /translation="MNTKPNTVTRREALLNALKGTVAAAVTAPVIVQATPDDSAKAET
                     TFVPENDYPFFGGEVPEGY"
     gene            complement(13146..14804)
                     /locus_tag="PLP58_07620"
     CDS             complement(13146..14804)
                     /locus_tag="PLP58_07620"
                     /inference="COORDINATES: protein motif:HMM:NF024240.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HRJ08418.1"
                     /translation="MKTILTLLLTLSTAIAAEQADVIVVTANPAGVAAAVAAAKSGAK
                     VIVLEESTHVGGIIAGGLTNTDIRKHGAVGGLYNDFKRRIVEHYTTTYGRDSKQLKVC
                     KDGNRFEAHVAEKVFRDMLAAEKNITLLQRHRVVAARVVGSDGVEKAATPGKRMDGAA
                     PEDFGPTVKLVSITAADLSKPGTQTEFRAAAFIDCTYEGDLAALAGVPYRVGRESRAD
                     FGEPHAGHIFIRFKDYNPLPGSTGEADAGIQAFCFRFHLTKVKENQVPVEKPEGYNRD
                     DYKHLLDDIRAGKIKRLRDAIQLYEMPNDKWEVNSEHNVAGGDAPSESLDLAEENWGW
                     PEAGPAERERIFKRYWTHNEGLIWLLGNDPECPQDLRDDVKQWGFPKDEFTDNRHRPH
                     HIYVRQGRRIWGEYNLTQNDAKPTFETGLPKRQPDGISIVEFEFDSHGVSKFDPRYPG
                     VRPGYVFIAHDPFQIPYRVVVPKCVDGLLVPVACSATHMGYSALRMEPVFMALGEACG
                     IAAMKAQASKTSVRQVDVKSVQREIVKRGGVILYENTAIVPEGL"
     gene            complement(14806..16101)
                     /locus_tag="PLP58_07625"
     CDS             complement(14806..16101)
                     /locus_tag="PLP58_07625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009960536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HRJ08419.1"
                     /translation="MKTIQTDVLICGGGCAGIAAALSSARNGARTLLIERAGFSGGII
                     TTVGLPYFDGLIDKPSGRFVVKGIALELLQRLGVAKEGAKHIDDLPPELVTKYWGSMW
                     IPNVEHFKIITDDLILKHGDNLSVLYHSTACDVEVKEGRIVAVVIANKDGLARVEAKQ
                     VIDCTGDGDIAHWAGCPTTKNKTLMPLTMHFRIGNVVPVKETKDAAKKVLIEAHKEGR
                     LVNFYGPGLIFAFAKDECYVHATRVPADATNAADFTRAEMQGRKDAWIIFNEWKAKVP
                     GFENSYYIMSGPFIGVRDTRRIVGLNVLTLEDLQKTTHHDDAVSTGCWFLDIHPPEVS
                     VDKPFTGYGFQPKPYDISYRTLVPQKVSNLLVAGRCHSASSEASASSRVTATAMALGE
                     AAGCAAALAMQAKSEVGTLDGVKVRETLAQQNAGPFSEA"
     gene            complement(16165..18378)
                     /locus_tag="PLP58_07630"
     CDS             complement(16165..18378)
                     /locus_tag="PLP58_07630"
                     /inference="COORDINATES: protein motif:HMM:NF024240.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HRJ08420.1"
                     /translation="MRAIFLSLIILPCLLCAEVIEADLLIVGGDESGCAAAVQAARLG
                     VKRIVLVNDIDWLGGQFCTQGIGPMDEWTLVNGKRVEFPATGAFAEILERIHTHNRQT
                     YGIAKPGNSWCGSNTIEPKPAARIFEEWLAPYAAQITILKGWEPVKVLVENGRVVGAE
                     FGQTGQTRLTVKAALTADSTDWGDVIRLSGAAYMAGPDLKSRFDEPSAPESLDPGGEQ
                     EMNPISWCPLLRETGKDSTIPKPARYDAHSFADWQKAPPWVDWDGSGGIYNMAGWCFY
                     THRRMIDRHHNNLAPGTEAVILNWPAHDYPLGTLPLHVVEALEKNEAGSSKKNIVDMT
                     PAQRRIIFADAKQRALEFVYWLQTAVHDRVGDYPQSFRHMKLADDYGTADHLPPKPYI
                     REGLRLDALYILREQDVRTEVEKPLWARSMPFDGVFGYQFNMDFHPTRRKFRDNDAAQ
                     PWQPKFFGTRNWNAHSDRTMFPLRGLVPVKMDGLLGCSKNTGVTSMVQSSLRLHGQMM
                     HVGTAVGTVAALALKDGLQPRDIATSPKRVREVQRTLVRHGTLIWPWHDVKPDDAHFE
                     AANLLTIAGIWRADPDSVFFKPDQAVTRRELAGALVRLIRSTPEAKEWPELPEQARFT
                     DVPADSADRAFIETMIAWGSFGSQETTFHPYQPVTWATLNRWLAALKLPTFKTLVHAK
                     VASYPLTRGECTDYLHRVLQQLGEGLPDRYDIDQDDAMPFDEDNNKVPDRLEPER"
     gene            complement(18380..18976)
                     /locus_tag="PLP58_07635"
     CDS             complement(18380..18976)
                     /locus_tag="PLP58_07635"
                     /inference="COORDINATES: protein motif:HMM:NF018190.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate-binding family 9-like protein"
                     /protein_id="HRJ08421.1"
                     /translation="MKRYIVQKTTEWAAACALTDFTLAWESRPSPKTEFRALHDPTHL
                     HFRFDCLDDDLVLPDADSEKERVLGSDRVEIFLAPDLSLQSYYCLEMSPRGEVLAYEA
                     RYYREFNWDWSLDGLDLDATIEGNLYTVTGRLPLATLRRLGVLHGSELFAGLYRAEFH
                     HRPDGSIHAGWMPWVNPQTEKPDFHVPASFGVLELVPS"
     gene            complement(19103..19906)
                     /locus_tag="PLP58_07640"
     CDS             complement(19103..19906)
                     /locus_tag="PLP58_07640"
                     /inference="COORDINATES: protein motif:HMM:NF020037.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HRJ08422.1"
                     /translation="MMQKPFPLKPRERPRRGAAEIARWRKARDAWMTSLEPASLFQRL
                     FDHLPGVYFFAKNRDGHLMFASQGLLQRYQMRDDSEFIGRTDFDINPDTMAQAYVEDD
                     KRLLAGQARIIERIELWWDRQGMPDWFLVTKLPLYDRRGRVQGVMGTLRRPDETERRL
                     PVFQTVAQAVERIRRDFSKPLKIEDIARSCGQSLRQLQRRFQSAFGITPQEFLIRTRV
                     LEATKLLEQTTLSASEIATRCGFVDPSSFAQHFRKRTGILPGVYRHARG"
     gene            19915..20145
                     /locus_tag="PLP58_07645"
     CDS             19915..20145
                     /locus_tag="PLP58_07645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ08423.1"
                     /translation="MDAPSGAARKQSHKDALDLPPVQPQVKGGQRMPCGLVQRPEPRV
                     GIAGIRARTCLALCHGQHGLDWEKGAKAALEG"
     gene            20225..20920
                     /locus_tag="PLP58_07650"
     CDS             20225..20920
                     /locus_tag="PLP58_07650"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009960538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004590 - orotidine-5'-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine 5'-phosphate decarboxylase"
                     /protein_id="HRJ08424.1"
                     /translation="MKPIVQISLDLTNIDEALETAALAMRAGVDWLEAGTPLILAEGL
                     HGVRALRKAFPTTPIVADLKTMDGGYLEAEMMAKAGATHVVVMARAHAETVKCVVKAG
                     KDFGCKVMGDNMICEDMVAGAKWLEDLGCDYIIHHIGYDERRGIAAAGHRMPSPLDKL
                     REVVQAVKVPVQAVGGLSLEQAIQCPKYGAPLVVLGAPLTIDADAFKTADGNLEASLR
                     MICEAVHAQEVRG"
     gene            21021..21662
                     /locus_tag="PLP58_07655"
     CDS             21021..21662
                     /locus_tag="PLP58_07655"
                     /inference="COORDINATES: protein motif:HMM:NF022142.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2490 domain-containing protein"
                     /protein_id="HRJ08425.1"
                     /translation="MRLPLLLMMLAAASARADEWWGWTMMELHRTETTNTGLFLGNRL
                     DADDGAYVQIISPRHKQRVLPWLDMGAGLSLLEIENLSEGGRFLQFRPELEINPHLDL
                     TPRLRLDLRNRMEWRWNEREEITIHRSRHRLQLGWKTPDGTGPLARVFASNEWLSDLH
                     RGQWWENRAVPLGLTFETSKHTELDLFYMIFSVRQQATWRHESVLGTYLRVRF"
     gene            complement(21679..22590)
                     /locus_tag="PLP58_07660"
     CDS             complement(21679..22590)
                     /locus_tag="PLP58_07660"
                     /inference="COORDINATES: protein motif:HMM:NF012437.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HRJ08426.1"
                     /translation="MSSRLVNISSGEASELQGVCMIGRNPDCTLQIADPGVSRRHAMI
                     REQNDGFWFFDLGSFNGSYVNGRRVTTAVKLRHNDVIELIEHQFRFEKGAEARDSTLI
                     AMDASTLARVKNGDAILLVSDIQGFTTLSERLDPARLAPIIGSWYSKTEGILTRFGAT
                     LDKFIGDSVFAYWTDISIPTRIAAMNAVGELQKSSLETHETHREALEPLGLRFATGAA
                     LHVGRVAYGGMSAREFTLLGDAVNLAFRIEALTRRLERPVIVSSEFLEGWPKGGTYCA
                     PLGQHEVKGRQQPVEVFAVERIPGMTA"
     gene            complement(22642..23316)
                     /locus_tag="PLP58_07665"
     CDS             complement(22642..23316)
                     /locus_tag="PLP58_07665"
                     /inference="COORDINATES: protein motif:HMM:NF013052.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SET domain-containing protein-lysine
                     N-methyltransferase"
                     /protein_id="HRJ08427.1"
                     /translation="MPAATPPRYWIVRRSSIHHRGLFARTDIPKDSPILEYVGEKITK
                     AESDRRGKALLEKSKNSGCAAVYIFTLNKRHDLDGGKAGNPARLINHSCDPNCEAYII
                     RGRVWIYSRKDIKAGDELTYNYGFDLETWEDHPCRCGSTRCAGHIVDEQYWPQLRRLV
                     KKREQERKKMAELEARAAVLQKRLDDLQPVAPGRKSRNGAHFKKVNGAAQPAAAADTR
                     RPRAAS"
     gene            23522..24841
                     /locus_tag="PLP58_07670"
     CDS             23522..24841
                     /locus_tag="PLP58_07670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ08428.1"
                     /translation="MLKTRPALFDSSKPLPILALASLAALTASPSAAQISDLSDLRQF
                     LSQNQVAGTPTTYEDKQAKIAANPNIFYVNPGPWGRLRCSYVYLEAPETLINNFPLPS
                     TRPRWSFPQAQLDELPSFFKTAGLSDALIQVLLAKENVVLEGPYAHVFPPLPDLEAIT
                     PESRAKIYTELSKYDANEYCVDPVLIVGQSVEEWYRTSKLRPQIIAKVKQMSYTRGET
                     IAFSDISAILNYAESDSEARAIFKAFTRTRAILVKMELDEQSNLAELLNYWTLGMGLR
                     RKDVEPLMQSIIDTNGVEALPLSHVLPSLVRKLLYTYPGMDLAKHGMLPDCHWTSLNF
                     FNHEPHEYLLDSRLATSQVLENFEPVQPPYKYGDILFFLSNTTGDAFHSCVHLADDLV
                     YTKNGRNLLSPWLIMRLEDVKRVYLYKGDGRVQAFRRKPGSELIPGN"
     gene            24989..25561
                     /locus_tag="PLP58_07675"
     CDS             24989..25561
                     /locus_tag="PLP58_07675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ08429.1"
                     /translation="MNLIVHLLLLAALSGLAGAAWVAQRPADLGGIRQAAPDDTRRPR
                     DVLDDLKQAAIKRSAVFEITEADLNHHLARVLQAGTLPPLNRWAAFARAAAALQPGQL
                     EVTLVWEVLGSPRTATFRFHIQRLEGSFRVVLTGGSYGRLRVPRGLLRPMLPALEQLG
                     LALEQEIQALFQMNQIQISEHKLVIDPRFP"
BASE COUNT         4368 a         8041 c         8320 g         4966 t
ORIGIN      
        1 atggcagcgc tccgccgcgc cgaggtccgc cagggcgctg ccattgccat cgccatcagc
       61 cgcgcgggca aagccgcgct ggtccgtggt gaatgccacg ttgagcgcgg cgcccacatt
      121 ggccgtgctg ccggcctgat cgagcaccgg gctgcccggc agcggcacga tcgtctgcgt
      181 ggcgccgcca tagttgcccg gcaaagacag ttcagggatg ccggtgagca cgttgccgct
      241 cgcgaaaaag ccgttgccgc cgagctgctg gaggaagatt cgcccggtga agttcaggct
      301 ggtgaaggcg ccgttttcaa acagctccgg tccggcgatg gtggcggcgt tgcccgcgaa
      361 gatggtgttg gcgacggaaa agctcccctt cacggacgcg ccgccgccat tggccgcgct
      421 atttaaccca agggtgcagt ggatcagcga caggctgccg tcgaaaaccg cgacgccgcc
      481 tcccaaggct gtcgcggtat tgctggccac agtgcattgc gtcagcgcga tgttcgcgga
      541 cgtgccgccg tcgatcgcgc cgccgttgcc ggcgcgcgcg gtgttcccgt tgagcgtgca
      601 gcggttcagc gtcgccgccg acacggtctc gatcgcgccg ccgaagtccg cctcgttgcc
      661 gctgagcgcg caatccgtca gcgacagggt gctgcccgtg ccgctgctga tcgcgccgcc
      721 gctgttgcca gccatcacct ggttcccgct gagcgcgcag cgggtgagcg tgaggttccc
      781 gtcgttgagg atcgcgccgc cgaagccgtt gaaggccgtg ccgccgccgt tgatgagctt
      841 caggttgtgc atcgccacgg tcctgacggc gccgacttgg aacaggcgga agttttgcga
      901 tccgccgttc acggtcacgc cattggcgag gccgccggca ttgatctcca ggtctctatc
      961 aatggtgatc ccgctgctga ggttgatcgt ctcgccgctc agcgccgggt cgaagttgat
     1021 gaccatgccg ccagtgccgg tgttgttcac cgcctgccgc agcgaaccgg caccggagcc
     1081 gttcgtgttc gtcaccacgt agccgcgctc caccgcgccg atgtccacgg tgccgttgaa
     1141 ggggaggaag gagccgcgct ggtcggtggt cacgccggcg gggatgacgg cgttgtcacc
     1201 cgcatcaatg gccgggctgc ccgcgagcgg cagcatcgtc ggcgtgcgcc cgccgtagtg
     1261 gccgagcggg gcgagattcg ccgcgccagt gatgacggtt gtgccattgg cgttgaggcc
     1321 ggacccggcg aggttgccga tgaggttctt gccggtagga gtgatggtgc tgtcgccgat
     1381 aaggctgaga aggctgtaaa tgtccggccc ggtcggcgcg gtgttcccgg cgacgatcgt
     1441 gttcgtcagc gtcagcgtgc cgccgtattg gtaaatcgca ccgccgccgt cgttggagga
     1501 attgcccgat agcgtgcatt gcgtcagcgt cagcgtgctt tggttctgaa tcgcgccgcc
     1561 gacgatattg gcggagttgc ccgagagcgt gcattgcgtc agcgtcagcg tgccgctgtt
     1621 ggagatcgcg ccgccgacga tgttggcgga gttacccgag agcgtgcatt gcgtcagcgt
     1681 cagcgtgccg ctgttggaga tcgcgccgcc gtagccgctg aaggacgtgc ccgtgccatt
     1741 cccgcccgtg agcgtcacgg ccttgagcgt gagattgccg cccgtgttca cctggaaaat
     1801 ccggctcgtc ccgccgccgg agaggaccac cccggcggtc acgttgctgc catccacggt
     1861 gatcaccgaa ttcatcggca ccacgatctc gctggcgagc acgatggggc cggtgaagcc
     1921 ggcggcgaag gtgatcgtcg tatcgggcac cgccgccgca tccgcgatgg cctgccgcag
     1981 cgaaccggcg ccgctgtccg ccgtggtgga gacgaccggc agcaggagcg tgccgcgctc
     2041 cgccgcgccg aggtccgcga ccacgctgcc attgccatcg ccatccgtgg gccgcgcatt
     2101 gccgcgctgg tccgtggtgg tgctgctgcc ggtggccgca tcgatcgcga ggctcccagg
     2161 ctgcggccgc atcgtctgcg tggggccgcc gtagtcgccg agtggatcga gattcgccgg
     2221 cacggggctg gcctcgatgc ccacgagcgg accctgcggg aattccgtca tcaccgtgtt
     2281 gttcttgccg atcaagttcg tgcccacggg cgtgatcgtg ccgccgccgc tggccggctt
     2341 gtggaggtcc ggcccggaga ccgaggcatt ccccgcgacg atcgagtagc ccagcgagag
     2401 cgtgccgccg agaacggaga tcgcaccgcc gttgccggcg aagccgttgg cggcggggtc
     2461 gccgttgttg atgaaattcc cggcgaacgt gccgtgcgtg atcgtgagcg ggccgaagtt
     2521 gcagatcgcg ccgccatcgc cggtggagga gttgccgacg aacgtgcaat tcgtcagcgc
     2581 gggcaccgtg ccgaagttgt aaatcgcccc gcccctggcg ctgccgtcga agatcggcgt
     2641 ggaaagctcg gcggccgtct tcgcggtcgc gttgccggcg aaagtgcagc ccgtcagcgt
     2701 gagcgtgccg ccggtgtttg cgatcgcccc gccgacgccg gagtctgcaa agccattgct
     2761 ccaaaaggcc cggttgttcg agagcgtgca atccgtcagc gtgagcacgc cggcgctcag
     2821 gatcgccccg ccgttgccgt cggtgacgga gtttgcggac aaggtgcagt tcgccagcgt
     2881 cgccgtgccc gtgaagaggt agatggcgcc gccgtcgccg acgcaggagt tctgctcgag
     2941 catgcagtta ttcagcgaca gcgtgccgga ggcgtggtga atcgcgccgc cacggtggag
     3001 tccggcagcg gtgttggttt gattgccgga gagcgtgcat ctcacgagcg tcagcgtgcc
     3061 ttggttgaag atcgcgccgc cgtcggtggc ggagttcccc gacaaggtgc agctcgtgag
     3121 cgtgagcgtg ccggcattct ggaaggcgcc gccggtcgtg gtgagattct ggcccccgcc
     3181 ctcgccaccc gtgagcgtga ggccggcgag gttcgcggtc ttccccggca gcacggtgaa
     3241 gagccggaac acgcccgtgc cgtcgcgcgt gatcgtgacg ccgccggcca ggctgctggc
     3301 gtcgatgctc acgctttttt cgatgacgag atccccgagc gaggagctga gcgtgatcgt
     3361 ctgcccgctc agcgccgcat cgaagatgat ggacccaagc ccgccgccat cgcgcagcgc
     3421 ctcgcgcagg gaaatgccga cgccgagcgt gccgttggga tcggcctcgt ccgcagcggt
     3481 ggtgacgacg atggggttcg gattggaaac ggtcggcgct gcctgcgcct catacgcgcc
     3541 gatgtccggc agcgcgccgg gtgtggcatc gccatcgcgg ctgcgctgga agccgcgctg
     3601 gtcgagatcg atcccgggca tcgccacgcc cgcatcgagc gccgggctgc ccggcagcag
     3661 cgccatcgtc tccaccggcc cgccattgtc ggcgagcgcg gcgagcttcg ggtcgccgga
     3721 ggtgaggttg ttcgagccgc tgccgatggt gccggagatg ttggcgttgg tcggcgcggt
     3781 gttgccggcg acgatgcagt tgttcagcgt cagcatgccg gcgttgcgaa tcgcgccgcc
     3841 ggtgttgccg gcggtgttgt tggagagcgt gcagtgcgtc agcgtcagcg tgccggcgaa
     3901 gtcaatcgca ccgccggtgt ccccggcgga attgcccgtg agcgtgcagt gcgtcagcgt
     3961 gagcgtgccg acgctggcaa tcgcgcctcc atcggcgaat ccgccgccag tggtggaatt
     4021 ccccgagaac gtgcagcgct ccagcttcag cgtgccgccg ttgccgatcg cgccgccgcc
     4081 ggtgctgaaa ttcgccccgc cgccattcgc gaacgtgagg ccgcgaaagg tgccgacgct
     4141 gtcgggagtg acgaagaaca gcccgtagct cgtcgccgtc ccatcatcga tgacgatgcc
     4201 cggcaccccg gtggcgtcga tcgtcacccc gcccgcgtca ttgatcgtga tggcggaccc
     4261 gttggaactc gtcgtgagcg agatcgtctg cccggcgagc aagggatcaa agacgatggt
     4321 gtccgggccg gccatcgccg ccgcatccgc gagcacctgc cgcagggagc cggtgccgga
     4381 gtcggcattg gtggtgacgg tcaaggtggc cgcgtgcagc gctggcgaga gcacaacgct
     4441 ggcgatggcg acgatgagcc tggttttcat ggcgtgtgaa gaacagcggg ggtggatgaa
     4501 tcgcggcagg tgctattttt tcccctcgaa gaagtcggcc gggtccacgc cttcgagctt
     4561 cacgatcacc ggggtgcagg tgccggactg gatgaccgac atcgtgccgc ggaaggtttt
     4621 cccggcttcc agtttgtatt tcgcgagata ctcagggccg ggataggtgc cgctgccgat
     4681 ctggaacgag tgctcccagg tcgcggccct ggcaccgagg tcgtccttga gcggggcgtc
     4741 gggcttgaag gtgaagcgaa cctcaaaacc ctcgtagccg gtggtctcgc tttgttgttt
     4801 ggacgcctcg gttttctcga tcctggtgat cgtcgccgag ccaggatggt cgtcgtaggt
     4861 gcaggggccg ccgacaatgg caggctggcc gccgcatgtg gagaggacgg agccaagaag
     4921 cagggccaga aaggcgaagg acatggtgtg aagcgttttc atgagatgga tcggcagaga
     4981 ttcacgcgct ggtcccgacc tcacttcggg cgtttcgact tgtccttgcc ctggtggagg
     5041 ttggcgatgg ccgcgttgat cttctgctcc atcttgctcc tgttgccggc ctccagttgc
     5101 tcgatggcct cgttgatctt ggcgatcacg tcctccttgt ccccgccctt gttgcctttg
     5161 ttggccttgg tgatgcggtt tttggcggat tccagcagcg gcatggggtc agcggccttt
     5221 ttggcggcct ggagctcatc gagcgccttg cgctgctggg gcagatcggc gcggacgacg
     5281 gtggtggtcc cgagaaaaag catcgcgcag gcaacgcggg cgagcagaca ggcaagatag
     5341 tgtttcatgg catggtgtgt gtttgttcga gaagggctgc acgagtgcgg atggcccttc
     5401 acacaccccc tgacacgccg gactggaatg gtaacaaagt tttcctcagc agccgcgcca
     5461 gggcccaaaa ctcacgaatc ccactcgacg atgaagccga cgctcggatt ggcccggctg
     5521 gtggggtcgt cgaaccaaaa gagcgtctgc gcgtggcttt tgatgctgat ggcgaagggg
     5581 ccttccaccg gctttcccgt ggtggtggcg taattcggct cgtccgtgcc ccagtcggtg
     5641 aacgtgaacg gctcgccggt gacccatcgg aagggctggc ctttctgttc agcgctcgcc
     5701 cccagccaga tgctcgcgga gcgcggcgcg ccatccagct tggtgccgaa agtcgcatca
     5761 atccacgcgt tctcttccct gctcgtgatc gtcaccagat gtccgcccat gctttcggcc
     5821 ttcgctttcg cctcatccca ggtcatcgcg ccgggcacaa accggtagcg gtggccggcg
     5881 aaggtgtgga cgccggcact tttgactgtt tcggtcctga ttggaccgcc gccgacaggc
     5941 cgcgaagcaa agaacacgcc ggccgaaatg acgatgaggc tggcaagaat gaccggaacg
     6001 agccatgtgc gggattggag gggcgcgcgg gcactcttgc ccgcctcttc attcgtcgcg
     6061 gccgcgccgc ccgatgattg actgcggaca ggagtgtccg cgctcccctc gatgccggtg
     6121 tccttgcccg gtccgatccg gctcacatcg gacttcacct cgctcgcggc ctggtagcgg
     6181 tccttcgggt cggtctgcat cgcgtggctc acgatgtcgt cccagtgcgg atcacacggc
     6241 gtgtgctgcg tcggtggttt ccatgccccg cgggggagct ggccggtgag catctggtag
     6301 atcatcacgc ccagcgcata gatgtccgcg cgatgatcca cctgcgccgc cgcgatgaac
     6361 tgctccggcg cggcgtagtc cggcgtgccc atgccggtgc cgctccgcgt gtggaagctg
     6421 ctttccacgt cgtggcgctt cgccagtccg aagtcggcga tcttgatgcg gccgtcgcgg
     6481 gtgagcatca cgttgctggg cttcacgtcg cgatggatga tgccgtgatc gtgcgcgaac
     6541 tgcagcgcgt cacaaatctg cggcagcagc gtgagcgcca tctcctgcgt catgcgcccg
     6601 ctgcggatca cgtccatcaa atccgcgccc tccaccagct ccatcacgat gtagaggtag
     6661 tccggccccg cctcgccgta gtcaatgacg ctgacgatgt tcggatggtt gagttttgcc
     6721 atcgccagag cctcacgctg gaagcgctgc tcaaagccat gatcccgccc ttgctcgcgc
     6781 cgcatgatct tgatcgcgac cgggcgctcc aaccgcaact gcgccccccg gtaaaccgcc
     6841 cccatgccac cgcgtctgat caaataatcc acccgatacg tgtccgccgg gagcagcccg
     6901 gtgagttcct ccggcaaagg aatctctgac gctgccgcag gcggcgccgt ccccgtctgc
     6961 gaggtctcca tcgcctgcgc catgaggcag cgcgggcaca gtccctcagt cgcctcggca
     7021 ggcagggccg cgccgcactg cggacaggtg tggagagatg cggatgagtc gctcatgagg
     7081 gaaaatgacg cgccggcgcc aattggtaac gaactttgtc gtgcggcgcc agaatatgtt
     7141 tttcggcctc gggagtgttg gaggatcagg gttcattact ccattactcc atcactccac
     7201 tgctccatca ctccactgct ccatcgctcc cccgcagtgt catcccgcca acgccgcgaa
     7261 caattcccgc atctccgcct ccaccatcgc cgcgtcatcg agcgtgccgg cgatctcctc
     7321 cttcacgcac tcgcgaaaac gccgccgcag gcggctgatg gccatcttca gcgccgccgc
     7381 gctcatcccg caggcctccg ccgccgcgcc ctggtctcca tgcgtggcat cgcccgtcag
     7441 ccagggcttc acccgctgga aaaacgcctc gcggccctcc ttcacgcact cctgcttcac
     7501 cgcatccatc gcgcgggcca gcgtggtcag cgcccactcg cggtcaaagg ccgcgtcggg
     7561 cgtcatctgc cgtgaatcgg gaatctcaca cacgccgctc gcgtcctcgt tgagagaaac
     7621 gtgcgccgcg ccaccgccgc gcttcatcct ccgcgccgcc tcccgctggt tggcgaggaa
     7681 atgcttcacc gcgccgagca gataggtgcg gaaacgcccg cgctcccgct ccgccgcggc
     7741 aatcgtgccg cccgccagca tctccgcgaa gaacgcatgg ctcatctccc gcgccgcgtc
     7801 cgcatcccgc agctcccagc gcaggaaggc gacgaccggc tcataatacg catcgcaaag
     7861 atccgccagc gcccgccgcc cgtcctccga ctccgttttg gcaaggccca cacgcgtcca
     7921 gcgcgtcgtg tgaaacgacg cggcgtctga aggtggatga ggctgctgtg acatcgtgtt
     7981 gcgccgcagt ctgccatagt agcgcaggct tgcgcaggaa aatgaggcgc ggcctgcgtg
     8041 ggtgtagatt cggttggcaa ccgaatcctt cgggggtgga ggtgcgcaaa gggactgcgg
     8101 cttcggtcgc caaccgaagc tacggaatgg tgctcgcatc cttcggcgga ttgcggccga
     8161 tgaagggatg ctgctccaaa tcgaagacct ggccgctgat ggggccgcat tcgtcggcga
     8221 ggagccaggc ggcgcaggcg gcgatctcct cgggccagag gatgcggccg gcgggggcat
     8281 agaccttcgg cagatcggca taccagtcgt ctttgaggcc gtgggagcgc ttccgtttgg
     8341 cttcgttttc ggtcagcacc cagccggggt tgatttgatt cacgcgcacg ccgtcctcgc
     8401 gcatgagcgt gtcgcccaga ttgcgggtga gggtcatgag agcgcctttg gagacgctgt
     8461 agggcatcaa attgggctcg ccgctccacg cgttgacgct gccaatgttg atgacgctgc
     8521 cgcgtgcttc acgcagatgc ggcaaggcgg cgcggatgag gaggtaaggc accacgcagt
     8581 tgatttcgag catgcggcgg aaccatttgg catctgtgtc gtgaatgttg ccgagaccga
     8641 cctgcgcggc gttgttcacc acggcgtcga gtttgccaaa ggcgctcaca gccttctcga
     8701 cgagcaattg tggcgcatcc tcttccgcga ggtctttgag cacgagcacc gccttgtccg
     8761 caccgagctc cgcgagcgtt tcctggccca aatcggtctc caggccgtgg atgagcactt
     8821 tcgcgccctc ggccacgcaa cgcttcgcga tggctttgcc gatgccggtg acgctgccgg
     8881 tgatgaggat gactttgttt tcgaggcgat tcatgcggag gcgaagcata acgagtcccg
     8941 cgccgttttc accacgacaa agtgtgtatg aattgcggca tggcaaaaag tggctgcggc
     9001 ttcagccgca ttctttgaat cggttcggct aaagccgaag ccactttttt attcctgccg
     9061 ttgcaagtcg aggctgaggt cgtagccgcc gtattcggcg accggatcgg cctggaggcc
     9121 aagggagagc gtggtttcgt tcgagttgcc gatggagatg ctgacttgct ggccgggacg
     9181 agttgccacg gtgggcatgg agagcatatc ggctccgcgg gtgttttgca gagagctttg
     9241 cagctttcgc aacctctctg ggccgatggc gctgtagttc tcaaagtcaa agaggtcggg
     9301 gaacaggtcg tgtgcctcgg cgtccgcgac agcggcatcg gataggtgca agagcttggc
     9361 ggtcatcttc acatggcctt cgggcgttgt ctcaggtgtc acgatggcca tgccgttcat
     9421 gcccggcgcg atctcccaat agcccagcac ggccgattca cccggcttga gccgcacttt
     9481 gcccttcgac aagattttga gcggttttcg aactgcgggt gctttttcgg ctttgggcgg
     9541 tgttggagtc gatatgctcg aggaagaagt ggaggcgact tgaggcgctg aaacgggctt
     9601 tttcgtctct ttcggttccc aagccgtgcg catgagccag aacgcgatca ggcacaagag
     9661 gaggcagaga aggatgatgg tgcggcgcat gggagaggaa agtagctgcg gcttcagccg
     9721 cattctcgct tcggttcggc taaagccgaa gccactttat cttcctaaac tcgatgcccg
     9781 cacccgcatg tcggccttgg aagccgatgc ggcctttggc tggcatttcg gaccacggcc
     9841 tgccttggag ccacttcggc atctcggagg cgtcgggatt cagtttgctg tctttccact
     9901 gcgtgaggtc gatttcgttc acgacatcgc cgttgagcac gacggtgatt ttggggcctt
     9961 gggcggtgag ggtcatgcgg ttccattcgc cggcgggttt gacggtgctc ttggtggcgg
    10021 gctgatggcc gaagaaggcg ccgcagcgcc agctggcggg cttttcctgc cattgcctgg
    10081 cgcgatcgtc acagatctgg atttcgatgc tggcgggcat ccagttcgtt tcgtcgctgt
    10141 tgtagaggaa gacgccgctg ttggctccgg gctcgaactt gaactcgaaa tcgagcacac
    10201 agtcgccgtg cgtggccttg gtccagatgt tttgatcctc ggtggcggtg aggaggccgt
    10261 cgcggtggct ccagacggct ccggggacct tcatgttggt caaatcgctc acgagcaggt
    10321 cattccacgc gctggagtcg ggatgggtgc gcagcggcag ggtgagtttc gcggcggcgg
    10381 ctttttcaga cgcggtgatg tcctccaaag gctcgcctcg ggcgaactgg cgcacgttgc
    10441 gcatgtattc cggcggcaca tcgtggccgt agccgcgcat gatgacgctc ttgaagtcga
    10501 gcttcatgag tttggcctga cgttcgagca gcgacgtctg atcgagcatc aactgccgga
    10561 actccgagcc gccggtcatc gcgtcatcgg ccacaagcag caggaagcgg ccgtctttga
    10621 tcggcatggc cgcgggcatg agctgaaagc cgccctcatg cagccagaac gcgtgaaagt
    10681 gctgcgtgat gaactcatcg cgacccgcga gcagcacgct gagcgtgtgc gcgccattcg
    10741 agtgcccgcc gatgacgctg cgctcccaat caatgttcgg cacctcggcg gcgagttttt
    10801 cgagcatcgt gcgatacgcc gcgctgacga cggcgaaatc ctcgaccgtc actggaagcg
    10861 ttttgccctt cggctggttc tttttgaaga gcggcacctg caccgcgatg aagtcctccg
    10921 cgcccacgat gtcgcgagcc atcgcgccgc catcctccgg cccgccgctg ccgcccttga
    10981 tgtaaacgaa gagcggatgc ttcgctgtcg tcgcgtagtt cgacggcagg atcgccccca
    11041 gccgcgccac cttcgccgtg cccgaaacca tgcccgcgag cgtgttcggc agctcgggga
    11101 agtcgaaggt gagtgccgca ttcggtttga gatcggccgc gagggcgctg gtggtgagaa
    11161 gggagagaag gagaagggtt ttcatgggct tgaaaagtgg ctgcggcttt agccgcatcg
    11221 ttttcatgat tcggctaaag ccaaagccac tttgttcagg cgagtggatc ctcagcgaac
    11281 caccgggctt ttccaatacg caacgcctgg caccttgagc tggccgggga ggaggacgcg
    11341 ctgataaagc cgcagcgtgg tctcacggcc tgcgaagtcg ccgaggtcga tcttgattgt
    11401 ttcaaaacgc acctcggtgc cgctgccctc gatggtcctg gcgagcacac gcttgttgtc
    11461 cgcatagaca ttcaactccc atgccttgcc gggatcggcg gccacctgaa gctccagcga
    11521 aggagcatcg cctagcttga gcttgcggcg cagcagcagg ctgcgggctt catcgcgcgg
    11581 ataggtgatg agcgtgtcgc catccagcgc ggtgtagccg cgaatgcctg gcatggcgcc
    11641 gacaccatcg ccgccgaaac ccgcccgctc cagggtccac tcgggattcc accactgcgt
    11701 cagatcggcg agctggaacc gctgggccgc ctgggtgacc actgcctcgg gaaggatgtg
    11761 aatctcatct ccgctgatct tgccgccctt tgcggtgatg attttttcgc cgatggccgc
    11821 ggtgcgcttc gccagtgtgc tgatcttttc atccacggct ggagtgaccg tcacgcggcc
    11881 aagtttttcg ccggtgatcc ggtcgttcaa ctgagccacc agctccgctg gcaacgcctt
    11941 catgcccttc atcgcggcaa tgatggaaat ggcggtggcg gcgctgttgt ccgtgtccgt
    12001 aaagtcggcg gcgcggcagg ccaggtcaat cgtggtccag aagtcgccgt tcccaaacca
    12061 gagacacgcg gcggcgagtc cgccattggg gacggcgttg ttcgaggccg gatactcgac
    12121 gcgccagcgg tcctccacgg cggcggcgat ggcttcaaag ctctgcccat tctccgccat
    12181 gtgaatgacg ctctcgacgc actggcggta gggcgaggac ggatcaatga gccgcaccgc
    12241 cttccgcagc acctcgcgga catcgctttc ggcaaacccg aggctgatgg ccccggcata
    12301 cagcaccgcg ccatcgagtg cctcgccata tccattgatc gcgcacagtt cgcgggcgag
    12361 ccgtcctgcc tcgttcggca ttcccggtgc cagcgcaccg tagagatcgg cgctgaagac
    12421 cgggccgatg gtccaccaca ggcggttgta gcgcgggtgg cccgcctcgc gggcggaaag
    12481 gcccttctcc agattggctc gcgtcaccat gctggagccg taggagccac aggcattctc
    12541 acgccactgt tcgccgagtt gctccagcgt caggccaatc ccatgcttct cgaaggcacg
    12601 cacggcgcac atttcataat accagtcatc atccacaaaa gcggctgtgt atggcttcgg
    12661 aaacgcgcgg atcggctcgg cggcggccac atgatgttcg aaaggcagcg tcagcaggac
    12721 ggcgatgagc tgaccatgcc agatggcgtt aacgcggtct tcatagtcgg cgcgcgtgag
    12781 cttgagcggt tccgctgcca gcgaagaagc gcaggtcagc aggatcagag cgatggtggg
    12841 cagggaagtt tttgtcatgg gaggaccgat tcgttgagcg ggatgacttc gtgccatccg
    12901 gcccacggtc ataatcctga cagtttctct tttcaatagc cctccggcac ctcgccgcca
    12961 aagaagggat agtcgttctc cggcacaaaa gtggtttcgg ctttagccga atcgtcaggc
    13021 gtggcctgca cgatgactgg cgcggtcacg gcggctgcga cagttccttt gagtgcattg
    13081 agcagagcct cgcggcgggt gacggtgttg ggtttggtgt tcatggtctt ggaaatggag
    13141 ccggatcaga ggccttcggg cacgatggcc gtgttttcgt agaggatgac accaccgcgt
    13201 ttcacgatct cacgctgcac ggacttcacg tccacctggc ggacactcgt tttcgaagcc
    13261 tgcgccttca tcgcggcgat gccgcaggct tcaccgagtg ccatgaagac aggctccatg
    13321 cgcagcgcag agtagcccat gtgcgtggcg ctgcatgcga cagggacgag caggccgtcc
    13381 acgcatttcg gcaccaccac ccggtaggga atctggaagg gatcgtgggc gatgaagaca
    13441 tagcccggac gcacgccggg atacctgggg tcgaacttgg agacgccatg cgaatcgaac
    13501 tcgaactcga caatcgaaat gccatcgggc tgccgtttgg gcaggccggt ttcgaaggtg
    13561 ggtttcgcgt cgttttgggt gagattgtat tcaccccaga tgcggcggcc ctggcgcaca
    13621 tagatgtggt gcgggcgatg gcgattgtcg gtgaactcgt ccttgggaaa gccccattgc
    13681 ttcacgtcgt cgcgcagatc ctgcgggcat tccgggtcgt tgccgagcag ccagatcaac
    13741 ccttcattgt gggtccagta gcgtttgaag atgcgctcac gctccgcagg accggcttcc
    13801 ggccagcccc agttctcctc ggcgagatcg agtgattcgc tgggcgcatc accaccggcg
    13861 acattgtgct cgctgttcac ctcccacttg tcgttgggca tctcgtaaag ctggatggcg
    13921 tcgcgcagtc gcttgatttt gcccgcgcga atgtcgtcga gcaggtgctt gtaatcgtcg
    13981 cgattatagc cctctggctt ctccactggc acctggttct ctttcacctt ggtgagatgg
    14041 aagcggaagc agaaggcctg gatgccagcg tcggcctcgc cggtggagcc gggcaacggg
    14101 ttgtaatcct tgaagcggat gaagatgtgc ccggcatgag gctcgccgaa atccgcacgg
    14161 ctttcgcgac cgactcgata cggcacgcca gccagtgcgg cgagatcgcc ctcgtaagtg
    14221 cagtcgatga aggctgcggc gcggaactcg gtctgcgtgc ccggcttgct caaatctgcc
    14281 gcggtaatgc tgacgagctt caccgtcggt ccaaagtcct ccggcgcggc tccatccatg
    14341 cgcttgccgg gagttgcggc cttctccacg ccatcgctgc cgacgacacg agccgcgacc
    14401 acgcgatgcc gctgcagcag tgtgatgttc ttctccgccg cgagcatgtc gcgaaacacc
    14461 ttctccgcga cgtgcgcctc gaagcgatta ccgtctttgc agaccttcaa ctgcttcgag
    14521 tccctgccgt aggtcgtggt gtagtgctcg acgatgcggc gtttgaagtc gttgtagagt
    14581 ccgcccaccg cgccgtgctt gcggatgtcg gtgttcgtca atccgcccgc gatgatgccg
    14641 ccgacgtgag tggattcttc gagcacgatg accttcgccc cgcttttcgc cgccgccaca
    14701 gctgcggcga ctccggcagg attggcggtg acgacgatga catcggcctg ctcggcggcg
    14761 atggcggtgg aaagagtgag aaggagtgtg aggatggttt tcatctcaag cttcgctaaa
    14821 aggccccgca ttctgctgcg cgagcgtttc acgcaccttc acgccatcga gtgtgccgac
    14881 ttcgctcttc gcctgcatag cgagcgcggc ggcgcagccg gcggcctcgc cgagggccat
    14941 ggcggtggcg gtgacgcggc tggaggcgct cgcttcgctg ctggcgctgt gacagcgacc
    15001 ggcgacgagc aggttgctca ccttttgagg cacgagcgtg cgatacgaga tgtcgtaggg
    15061 ctttggttgg aagccgtagc cggtgaaggg tttatcgacg gacacttcgg gcggatggat
    15121 gtcgaggaac cagcagccgg tggagaccgc gtcgtcgtgg tgcgtggtct tttgaaggtc
    15181 ttcgagcgtg agcacgttca gccccacgat gcggcgcgtg tcgcgcacgc cgatgaacgg
    15241 cccgctcatg atgtagtagc tgttttcaaa gcccggcacc ttcgccttcc actcgttgaa
    15301 gatgatccac gcatccttgc gcccctgcat ctcggcgcgg gtaaagtcgg cggcattcgt
    15361 cgcatcggca ggcacgcgcg tggcgtggac gtagcactcg tccttcgcga aggcgaaaat
    15421 cagcccgggg ccgtagaagt tcacgaggcg accttccttg tgcgcttcga tgagcacctt
    15481 cttcgccgcg tccttcgtct ctttcacggg gacaacattg ccgatgcgga aatgcatcgt
    15541 cagcggcatc agcgtcttgt ttttcgtcgt cggacaaccc gcccaatgcg cgatgtcgcc
    15601 atcgccggtg caatcaatga cctgcttcgc ctccacgcga gcgaggccgt ccttgttcgc
    15661 aatgaccacg gcaacaatgc gaccttcctt tacctccacg tcgcaagccg tgctgtgata
    15721 gagcacgctc aagttgtcac catgtttcag gatcaggtca tcggtgatga tcttgaaatg
    15781 ctcgacgttg ggaatccaca tgctgcccca atacttcgtc accagctccg gcggcaggtc
    15841 atcaatgtgc ttcgcgcctt ccttcgccac gccaagccgc tgcagcagct ccagcgcgat
    15901 gcctttcacg acaaatcgac cgcttggctt gtcaattagc ccgtcgaagt aaggcagccc
    15961 caccgtggtg atgatgccgc cggagaagcc agcgcgttcg atgagcaatg tcctggctcc
    16021 attgcgagcg gaggaaagcg ccgctgcaat gccggcacag ccgccgccac agatgaggac
    16081 gtcagtttgg atggttttca tgatggccga gcataacgaa cacgcccggc ttcatgccac
    16141 gacaaagagt gtgcgaaatg cgactcagcg ttccggctcc agccggtccg gcaccttgtt
    16201 gttgtcttca tcgaagggca tcgcgtcgtc ctggtcgata tcgtagcgat ccggcaatcc
    16261 ttcgccgagt tgttgaagga cgcggtggag gtagtcagtg cattcgccgc gggtgagcgg
    16321 ataactggcg accttggcat ggacgagcgt tttgaaggtc ggcagtttca gcgcggccag
    16381 ccagcggttg agcgtggccc aggtcacggg ctggtaggga tggaaggtcg tttcctgcga
    16441 cccaaagctg ccccaggcga tcatggtttc gatgaaggcg cggtctgcag aatcagcggg
    16501 gacgtcggtg aagcgcgctt gttcgggaag ttcaggccac tccttggctt ccggtgtgga
    16561 acgaatcaac cggacgagcg cgccggcgag ttcgcgccgc gtgacagcct gatccggttt
    16621 gaagaagacg ctgtcaggat cggcacgcca gatgccggcg atggtgagga ggttggcggc
    16681 ttcaaagtgc gcgtcgtcgg gcttcacatc gtgccagggc cagatgagcg tgccgtgacg
    16741 aacgagcgtg cgctgcacct cgcgaacgcg tttgggcgag gtggcaatgt cgcgaggctg
    16801 gaggccgtct ttgagcgcga gcgcggccac ggtgcccacg gcggtgccga cgtgcatcat
    16861 ctggccgtgg agacgcaggg acgactgcac catgctcgtc acgccggtgt ttttcgagca
    16921 gccgagcagg ccgtccatct tcaccggcac gagtccgcgc agcgggaaca tggtgcggtc
    16981 gctgtgcgcg ttccagttgc gcgtgccgaa aaacttcggc tgccagggtt gcgcggcatc
    17041 attgtcgcgg aacttccggc gcgtgggatg gaagtccatg ttgaactggt agccaaacac
    17101 gccatcgaag ggcatcgagc gcgcccacaa cggcttttcc acctcggtgc gcacgtcctg
    17161 ctcgcgcagg atgtagagcg cgtcgagtct caggccttcg cggatgtagg gcttcggcgg
    17221 caggtgatcg gcggtgccgt aatcgtcggc gagcttcatg tggcggaagg attgcggata
    17281 gtcacccacg cggtcatgca cggctgtttg cagccaatag acaaactcca acgcccgctg
    17341 tttcgcgtcg gcgaaaatga tgcggcgctg cgcgggcgtc atgtccacga tgttcttctt
    17401 cgatgagccg gcttcgttct tctccaaagc ctcgacgaca tgcagcggca gcgtgcccag
    17461 tggataatca tgcgcaggcc agttcaggat caccgcttcc gttcccggcg cgaggttgtt
    17521 gtgatggcgg tcaatcatgc gccggtgcgt gtagaagcac cagccggcca tgttgtagat
    17581 gccgccgctg ccgtcccagt ccacccatgg cggggctttc tgccagtcgg cgaaggaatg
    17641 cgcatcatag cgtgcgggtt ttggaatcgt gctgtctttg ccggtctcgc gcagcagcgg
    17701 gcaccaggaa atcggattca tctcctgctc gccgccggga tcgagcgatt cgggtgcgct
    17761 cggttcatcg aaacgactct tcaaatccgg cccggccatg taagccgcgc cgctgagccg
    17821 gatgacgtcg ccccagtcgg tggagtcggc ggtgagcgcg gctttgaccg tcagacgggt
    17881 ctgacccgtc tgaccaaact ccgcgccgac gacacgcccg ttttccacga gcaccttcac
    17941 cggttcccag ccttttaaga tggtgatctg tgccgcatac ggagccagcc attcctcgaa
    18001 aatccgcgcc gcgggcttcg gctcaatcgt gttgctgccg caccaactgt tgccgggttt
    18061 cgcgatgccg taggtctggc gattgtgcgt gtgaatgcgc tccaggattt cggcgaaggc
    18121 gccggtggct ggaaactcga cgcgcttgcc attcaccagc gtccattcgt ccatcggacc
    18181 gatgccctgc gtgcaaaact gtccgccgag ccagtcgatg tcattgacga gcacgatgcg
    18241 cttcacgccg agccgagcgg cctgcaccgc agcagcgcag ccggattcat cgccgccgac
    18301 gatgaggagg tcggcctcaa tgacctcggc acacagaagg catggcagaa tgatgaggct
    18361 caggaaaatg gcgcgcatat caggagggaa ccagttcgag cacgccgaag ctggcgggga
    18421 catggaagtc gggtttttcg gtctgcggat tcacccaggg catccagccg gcatggatgc
    18481 tgccatcggg ccggtgatga aactcggcgc ggtagaggcc ggcgaagagt tcgctgccgt
    18541 gcaagacgcc aaggcggcgc agggtggcga gagggagacg gcccgtgaca gtgtagaggt
    18601 ttccttcgat ggtggcgtcg agatcgagtc cgtcgaggct ccagtcccag ttgaactcac
    18661 gatagtagcg cgcctcgtag gccagcacct cgccacgcgg gctcatttcg aggcagtagt
    18721 aggactgcag gctcaagtcc ggcgcgagga agatttccac acgatcggaa ccgagcacgc
    18781 gctccttttc cgaatccgcg tccggcagca ccagatcatc gtcgaggcag tcgaagcgga
    18841 aatgaagatg tgtaggatca tgaagggcgc ggaactccgt tttcggcgac gggcggctct
    18901 cccacgccaa ggtgaaatcc gtgagagcac aggcagcggc ccactcggtg gttttctgga
    18961 cgatgtatcg cttcatgggg tgtgggtcag tttcccgcca ttctcgaaat cactgccaag
    19021 tttcgcgatc aggctgcatg taatcaaaac cagagcaagg aagccgacaa cagtctttcc
    19081 ttgtcagcgc gacagttcca agtcatccgc gcgcatggcg gtaaacacct ggcaagatgc
    19141 cggtgcgttt gcgaaagtgc tgggcgaaac tgcttggatc gacgaagccg caacgggtgg
    19201 cgatctcgct ggcggagagg gtggtctgct ccagcagctt ggtggcctcc agcacgcgcg
    19261 tgcggatgag aaactcctgc ggggtgatgc caaaagcgct ctggaagcgg cgctggagct
    19321 ggcgcagcga ctggccgcag gatctggcga tgtcctcgat cttgagcggt tttgagaaat
    19381 cacgccggat gcgctccacg gcctgcgcca cggtttggaa gacgggcagc cgccgctccg
    19441 tctcatcggg acgccgcagc gtgcccatca caccctggac gcggccccgg cggtcgtaga
    19501 gcggcagctt cgtcacgaga aaccagtccg gcatgccctg gcggtcccac cacagctcga
    19561 tgcgctcgat gatccgcgct tgtccggcca gcaggcgctt gtcatcctcc acataggcct
    19621 gcgccatggt gtcgggattg atgtcgaaat ccgtgcggcc gatgaattcg gagtcgtcac
    19681 gcatctggta gcgctgcagc aggccctggc tggcaaacat gaggtgccca tcacggttct
    19741 tggcgaaaaa atagacgcct ggcaggtggt caaacagccg ctgaaacaag gaggcgggtt
    19801 ccagcgaggt catccacgca tcgcgggcct tccgccagcg ggcgatctcc gccgcgccgc
    19861 gtcgggggcg ttcgcggggc tttaacggga agggcttctg catcatgatg gcgcatggat
    19921 gcgccctcag gcgcggcccg caagcagtcc cacaaggatg cgctcgattt accgccggtg
    19981 cagccgcagg tgaaaggcgg ccagcgcatg ccatgcggtc tcgtccagcg acccgaacca
    20041 agggtgggca tagcgggcat ccgtgcgcga acctgtctgg ccctttgtca cggccagcac
    20101 ggcctcgact gggaaaaggg cgccaaggcg gcgctggaag ggtgagcccc cgtcgcattt
    20161 cgcatgatct ttgtcgtgac gcgggagcct gcttttgctt tgctgcggct ccgccgaccc
    20221 ccgcatgaaa cccatcgtcc aaatcagcct cgacctcacg aacatcgacg aagccctcga
    20281 aaccgccgcg ctggccatgc gcgcgggtgt ggactggctg gaggccggca cgccgctcat
    20341 tttggcagag gggctgcatg gcgtccgcgc gctgcgaaag gccttcccga ccacgcccat
    20401 cgtcgccgac ctcaaaacga tggatggcgg ctacctggag gccgaaatga tggccaaggc
    20461 cggtgccacg catgtcgtcg tgatggcccg tgcgcacgcc gagaccgtga aatgcgtcgt
    20521 gaaggccggg aaggacttcg gctgcaaggt gatgggcgac aacatgatct gcgaggacat
    20581 ggtcgccggc gcgaaatggc tcgaagacct cggctgcgac tacatcatcc accacatcgg
    20641 ctacgacgag cgtcgcggca tcgccgccgc cggccaccgc atgcccagcc cgctcgacaa
    20701 gctccgcgag gtcgtccaag cagtgaaagt gcccgttcaa gccgtcggtg gcctcagtct
    20761 cgaacaagcc atccagtgtc ccaaatacgg cgcgccgctc gtggtgctcg gcgcgccgct
    20821 gaccatcgac gcggacgcct tcaaaaccgc cgatggaaat ctcgaagcct ccctgcggat
    20881 gatttgcgag gcggtgcatg cgcaggaggt gcggggctga atttccaaac agcgcgatat
    20941 tgtcacacac atcgggatgc cctgcctgac tcgcgcgcca ggatcgggtg tgctggcatc
    21001 cgatatgctg gcattttgtc atgcgccttc ctcttcttct catgatgctc gccgccgctt
    21061 cggcgcgcgc tgatgaatgg tggggatgga cgatgatgga actccaccgt acggagacca
    21121 cgaacacggg cctttttctc ggcaaccgcc tcgacgcaga tgacggggcc tatgtgcaga
    21181 tcatcagccc gcgccacaaa cagcgggtgc tgccctggct ggacatgggc gccgggcttt
    21241 cgcttctgga aatcgagaat ttgagcgagg gcgggcgctt tctccagttc aggccagaac
    21301 tggagatcaa tccgcatttg gacctgactc cgcgcctgcg gctggacctg cgcaaccgca
    21361 tggagtggcg ctggaacgag agggaggaaa tcacaatcca ccgctcccgt caccgtctcc
    21421 agctcggctg gaagacacct gatggaaccg gtccattggc gcgtgttttc gccagcaatg
    21481 aatggctcag cgacctgcat cgcggccagt ggtgggaaaa ccgcgcggtg ccgctcggtc
    21541 tgacattcga gacctcgaaa cacacggaac ttgacctttt ttacatgatc ttctccgtcc
    21601 gccagcaggc gacgtggcgg catgagtccg tgcttggcac ctacctgcgc gtgcggtttt
    21661 gagggcaggg cggcggcctt atgcggtcat gccggggatg cgctccacgg cgaagacctc
    21721 gaccggttgc tggcgccctt tcacctcgtg ctgccccagc ggggcgcagt aagtgccgcc
    21781 tttgggccag ccttcgagga attcggaact gacgatgacg ggacgctcca gcctgcgggt
    21841 gagcgcctcg atgcggaagg ccaggttcac agcgtcccca agcagggtga actcgcgcgc
    21901 gctcatgcct ccataggcga cacgaccgac gtgcagggcg gcaccggtgg caaatcgcaa
    21961 acccagcggt tcgagcgcct cgcggtgggt ctcatgagtc tccagcgagg acttttgcag
    22021 ctcgccgacg gcgttcatgg cggcgatgcg cgtggggatg gagatgtccg tccagtatgc
    22081 gaaaacggag tcgccgatga atttgtcgag cgtggcgccg aaccgggtga ggatgccctc
    22141 ggtcttgctg taccaggagc cgatgatggg tgcgagccgc gcgggatcga gccgctccga
    22201 gagggtggtg aagccctgta tgtcgctgac cagcaggatg gcatccccgt tcttcacgcg
    22261 cgccagggtc gaggcgtcca tggcgatcag agtgctgtcc cgcgcctcag cgcctttttc
    22321 aaaacggaac tggtgctcaa tgagttcgat cacatcgttg tggcgcagct tgacggcggt
    22381 ggtgacacgc ctgccgttga catagctgcc gttgaaactg ccgaggtcga agaaccagaa
    22441 gccgtcgttc tgctcccgga tcatggcgtg gcgcctcgac acaccgggat cggcgatttg
    22501 cagggtgcag tccgggttgc gcccgatcat gcagacgccc tgcagttcgc tggcttcacc
    22561 ggagctgatg ttgacgaggc gggagctcac ggcggtgaag taaggacgcc ggagcggctt
    22621 tgcaagtcac aaggcctgcg gtcacgaggc agcgcggggg cggcgtgtat cggcggcggc
    22681 ggcgggctgg gcggctccgt tcactttttt gaaatgcgcg ccattgcggc ttttccttcc
    22741 gggcgcaaca ggctgcagat catccaggcg cttttgcagg acggcggccc tggcctcgag
    22801 ttccgccatc tttttgcgct cctgctcgcg ttttttgacc agcctgcgca actgcggcca
    22861 gtactgctcg tccacgatgt gtcccgcgca gcgcgtggaa ccgcagcggc aggggtggtc
    22921 ctcccaagtc tccagatcga agccgtaatt gtaagtcagc tcgtcacccg ccttgatgtc
    22981 cttcctcgag taaatccaca ccctgccacg gatgatgtag gcctcgcagt tcggatcgca
    23041 ggagtggttg atgagccgcg cgggattgcc cgccttgccg ccgtcgagat cgtggcgctt
    23101 gttgagggtg aaaatgtaaa ccgccgcgca gccggagttt ttcgacttct ccagcagcgc
    23161 cttgccgcgg cggtcgcttt ccgccttggt gattttttcc ccgacatatt cgagaatggg
    23221 gctgtccttc ggaatgtccg tgcgggcaaa aagtccgcga tggtggatgg aggagcggcg
    23281 gacgatccag taacgggggg gggtggctgc gggcatgaag atcgggagag cggatgtgat
    23341 gccgaccgga atgacaaagt caagctggcg ccaaaaatct tggcgggtca tccgttgtag
    23401 cagcgtcatg cgtgcttttt ttcggagaag gcgaggcctg gcccggaaaa aaccgtccgg
    23461 tagaaaatat ttcgtgcaac cctccggccc tgttgaaggt tttcatctcc ccccttgcct
    23521 catgctcaaa acccgacccg ctttgttcga ttcgtcgaag ccgctaccta tcctcgctct
    23581 agccagcctt gccgcactca cagcctcacc ctccgccgcg caaatctccg atttgagcga
    23641 cctgcggcag tttctctccc aaaaccaggt cgctggcacg cccacaacct atgaggacaa
    23701 gcaggccaaa atcgccgcca atcccaacat tttttatgtc aatccagggc catggggaag
    23761 gctgcgctgc tcctatgttt acctcgaggc gccggagact ttgatcaata atttccccct
    23821 gccgagcacc cgcccgcgct ggagctttcc tcaggcccag cttgacgaac tgccttcttt
    23881 tttcaaaacc gccggtcttt cggacgccct catccaggtg cttctcgcca aggaaaacgt
    23941 ggtcctggag ggaccctatg cgcatgtttt ccctcccctg cccgacctgg aggcgatcac
    24001 gcccgaaagc agggctaaaa tctacaccga gctttccaaa tacgacgcca acgagtactg
    24061 cgtggacccg gtgctcatcg tcggccagtc ggtcgaggaa tggtaccgga ccagcaagct
    24121 gcgcccgcag atcatcgcca aggtcaagca gatgtcctac acccgcggcg agaccatcgc
    24181 cttcagcgac atctccgcca ttctcaacta tgccgaaagc gactcggagg cgcgcgccat
    24241 tttcaaagcc ttcacgcgca caagagcgat tctggtcaaa atggagctgg atgagcagtc
    24301 aaacctcgcg gagctgctca actattggac tctcggcatg ggtctccgcc gcaaggatgt
    24361 ggagccgctg atgcagtcca tcatagacac gaacggcgtg gaggcgctgc cgttgtccca
    24421 cgtcctgcca tccctggttc gcaagctgct ctacacctac cccggcatgg acctggccaa
    24481 gcacgggatg ctgcccgact gccactggac ctcgctgaat ttcttcaacc atgagccgca
    24541 tgagtatctc ctcgactcgc gcctggccac aagccaggtg ctcgaaaact tcgaacccgt
    24601 gcagccccct tacaaatatg gcgacattct tttcttcctg agcaacacca ccggcgacgc
    24661 cttccattcc tgcgtgcatc tggccgacga cctcgtttac acgaaaaacg gccgcaacct
    24721 tctctccccc tggctcatca tgaggctcga ggatgtgaaa agagtttatc tctacaaggg
    24781 tgacgggcgc gtgcaggcct tccgccgcaa gcccggcagc gagttgatcc ccggcaactg
    24841 atccaaccca tgcacagccg cggcgcgggg cggcatgcgc caggacaatt tgagcttggg
    24901 tttcacccgc cccaaggcga gtgtgttccg gcttcccaag gtacgggttt cgtgataaaa
    24961 agccgccctc cttcacagac cacccgcttt gaacctgatc gtccatctgc ttttgctcgc
    25021 cgccctcagc ggcctcgccg gcgccgcctg ggtggcccag cgccccgccg acctcggcgg
    25081 catccgccag gccgcccctg atgacaccag gcgtccgcgt gacgtgctcg acgacctcaa
    25141 gcaggccgcc atcaagcgct ccgccgtctt tgaaatcacc gaggccgacc tcaaccacca
    25201 cctcgcccgc gtcctccagg ccggcaccct gccgccgctc aatcgctggg ccgcatttgc
    25261 ccgcgcggcg gccgcgctgc agcccggcca gttggaggtc acactggtct gggaagtgct
    25321 cggcagcccc cgcaccgcga ccttccgctt tcacatccag cgccttgagg gcagcttccg
    25381 cgtcgtcctc accggcggca gctacggccg cctccgggtg ccgcgcgggc tgctgcgccc
    25441 catgctgcca gccctcgaac agctcggcct ggctttggag caggagatcc aggccctctt
    25501 ccagatgaac cagattcaga tctccgagca caagctcgtc atagacccgc gctttccgta
    25561 acccacgcca tgtcacgccg agtcctgccc cttttgctcc tcagcctggc cgtgcaaacc
    25621 gcccggccgc aggagcggca ggattcagcg gcggagcgcg gccaggccgc cgccattcag
    25681 cctcccgcac tcccc
//
LOCUS       DAOMYJ010000053        25543 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Prosthecobacter sp. isolate AMR_MDS_5629
            NODE_696_length_25543_cov_23.524874, whole genome shotgun sequence.
ACCESSION   DAOMYJ010000053 DAOMYJ010000000
VERSION     DAOMYJ010000053.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867850
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prosthecobacter sp. (wastewater metagenome)
  ORGANISM  Prosthecobacter sp.
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Verrucomicrobiaceae; Prosthecobacter.
REFERENCE   1  (bases 1 to 25543)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 25543)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.30
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:34:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,742
            CDSs (total)                      :: 4,684
            Genes (coding)                    :: 4,676
            CDSs (with protein)               :: 4,676
            Genes (RNA)                       :: 58
            tRNAs                             :: 55
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25543
                     /organism="Prosthecobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_696_length_25543_cov_23.524874"
                     /isolate="AMR_MDS_5629"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1965333"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..250)
                     /locus_tag="PLP58_07680"
     CDS             complement(<1..250)
                     /locus_tag="PLP58_07680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ08430.1"
                     /translation="MKNITLIRSAAAAGILSLTHCAGTSTPESAPAADTAASTTAPAA
                     SSASSGITDPSAAQPTHLSKAAAGVKDGVTTRKDIIRKL"
     gene            358..2070
                     /locus_tag="PLP58_07685"
     CDS             358..2070
                     /locus_tag="PLP58_07685"
                     /inference="COORDINATES: protein motif:HMM:NF019474.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HRJ08431.1"
                     /translation="MKIPLFPALAVVSVIAHAANAQGPNTRVPEGVRAHRDIVYVEGG
                     HERHKLDLYLPEKSQGPLPLIIWVHGGGWMNGSKDGCPPLRAGYVESGYAVASINYRL
                     SSHAVFPAQIEDCKAAIRWLRAQAGKYGLDAGRFGVWGSSAGGHLAALLGTSGDAREF
                     DTGAHLDQSSRVQAVCDYYGPTDFTVFVTTPGYESHASDTSPEARLLGGAVMRNKDKA
                     ARANPITHISNDDPPFLIVHGDQDPTVPINQSQLLFRELGAAGVNAHFHTIKGAGHGG
                     PGFSGEDIGQMVAAFFNARLKDGISGTGTLATQSTIAPVGGPRAGRDGVRGRSAPEPR
                     EASTAKGFKLEGEHWTYREGDLTMSGILLKPEGKGPFPAVLISHGLGGSARSFGLTKA
                     REMVRWGMVCIAPDYTHTGAGGDRVQFGASAENVRRASTCLEILRGMPDVDGARLAAY
                     GHSMGGFVTIGLAASAPGLLKAAAITGSGISPQEGHPAPSTSTAEKVRTPFLMLHGAN
                     DTTVRPEQSASLKQVLDSNGVVNDRLIADGQGHPIDQTMREEVFRLIREWFIKNGALG
                     SKSK"
     gene            2153..2857
                     /locus_tag="PLP58_07690"
     CDS             2153..2857
                     /locus_tag="PLP58_07690"
                     /inference="COORDINATES: protein motif:HMM:NF028441.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein-sorting protein"
                     /protein_id="HRJ08432.1"
                     /translation="MMNRRIFLASLAAAPLARAQDPTPTAEEVLRLVRKSYALQDHKM
                     TGRLRDGNSGREEPLELTLTQSVMRFRFMNPPAEIVHLDLTTEPATLWEVKTGGSSKV
                     PLSNASNAVRGMDFNYEDLSQRFLYWKNAKMMDANARLSAAGIKCWLVRVTAPDTKGP
                     YYTVDLWVHKDSGGVAKMEAYNAPSKLVKRFEVTKMWKVGEATALREMRVQSFNPLDG
                     SRKGTTYMTMDKPEKQ"
     gene            complement(2900..3253)
                     /locus_tag="PLP58_07695"
     CDS             complement(2900..3253)
                     /locus_tag="PLP58_07695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ08433.1"
                     /translation="MFQTGQKVVCINDQFDPWVYDLYRALPRKGRAYTVRAVRSGRSN
                     PVFSLNDDAEIKLTGAEFDILILLEELQNPDDPHSSVKQELGFRSERFAPLETEEEEA
                     EDEAGELIGAGAKAW"
     gene            complement(3287..4906)
                     /locus_tag="PLP58_07700"
     CDS             complement(3287..4906)
                     /locus_tag="PLP58_07700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ08434.1"
                     /translation="MTRLLLLALVLSTASCIGPPKTPEAREPLQWRFTQSARARLGGH
                     LAEAGAAWERLHSPGSNRRERAEAEERYEKALAAFLKGWSRAQAPRRWQDGAAFAAHR
                     VRIQTRNHDTREITPRVLADLRLASEVRTGKAGSLAETDGLGVPLVGRVFESDQHPLL
                     PRNGAHLTLTALLEFEKSVPGQPRECRLRLVNPLQQPRVRIEGKDTPLAANYTAPKAL
                     ALDAGFLKKFSLTGLLYPEKTLDDSRLYQLDPYDPRRIPVVFVHGLMSDPHIWYPCIN
                     ALCADPVLRAHFQPWYFLYPTGMYVPATSRRLRESIAEARDRLDPGGTSAAMNQMVLV
                     GHSMGGLLSRMQAVDSGMEFWNAHFTKPPEEMRLSESGRERLRTGFIFEKRPEVARLI
                     FIATPQRGSDLADKGIVMRLAKLIRLPVDSMLVVTELLSGNTDALQPQIRDWGVFSFL
                     SLSTLSPRHPYIQALNARPIPVPHHSIIGRVGGRTLEDSSDLVVPYRSAHLPTGSERV
                     VPHWHGCVEQPEVIAEVLKRLRQHLRESGRI"
     gene            complement(4903..5688)
                     /locus_tag="PLP58_07705"
     CDS             complement(4903..5688)
                     /locus_tag="PLP58_07705"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009961021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HRJ08435.1"
                     /translation="MPLPLSISIISKNEEANLRRCLESAADLAAEIIVVDSGSTDATR
                     AVAREFGARVEHQDWLGHRDQKNVALRLCTQPWVLALDCDEELSPELRRSIQDFFAQG
                     GHEQNDGAIMPRKVFFLGRWITHGDWYPDRKLRLFKRAGALWGGSPEHDKVVLPEGAR
                     PCLILDGDLHHYSFRDMSHYISKINVFADVFLQRQEADGVRWALLPTLTRPLWRFFRA
                     YVLRRGFLDGFPGLWIAAATAFFAFVRYSRKYEAEHPAKPPQS"
     gene            complement(5745..6398)
                     /locus_tag="PLP58_07710"
     CDS             complement(5745..6398)
                     /locus_tag="PLP58_07710"
                     /inference="COORDINATES: protein motif:HMM:NF012355.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="plastocyanin/azurin family copper-binding
                     protein"
                     /protein_id="HRJ08436.1"
                     /translation="MSAPVKSEESGSIWSILNVLIAVTLGGLVLFWAGAFLTGVVSQT
                     TGSTEAGAETAASAPATPAAPAASAATPSAPAAAATAPAVPAPTGEVIEITIKPDLTN
                     PLAYDTKEFTVKAGAKVKLTFNNVHPTVPQPHNIVIGALGSKDKLMGLAMQMATDPAG
                     MAKGFIPEAPEVLFHTKLLQPNQSEVLEFTAPAAGDYPYLCTFPGHAVLMNGVMKVQ"
     gene            6786..7799
                     /locus_tag="PLP58_07715"
     CDS             6786..7799
                     /locus_tag="PLP58_07715"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009960245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0047952 - glycerol-3-phosphate
                     dehydrogenase [NAD(P)+] activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0046167 - glycerol-3-phosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HRJ08437.1"
                     /translation="MPTPDLPRAIVLGAGSWGTALAAMLGMRGVRTQFWGRDAGLMRE
                     MRETRRNARYLPALELPESVSVATSLAGLESAEMLVFVTPSKGVRETARLVAESGLAA
                     EARVILSCAKGIELDSGKRMSQILAEHFPGKALAVLTGPNHAEEVAQQLATAAVVACE
                     DEAVARAVQSCLTLPFFRTYTSDDVTGVEWAGAMKNPYAIAAGVALGLRLGDNAIAAL
                     VTRALAEMVRLGTAMGGRVESFYGLAGVGDLMGTCYSEHSRNHRLGRQLGEGRPLAEI
                     LGSSRMVTEGVPNTASLHQAAKAAGVRTPLLDEVHAMLYEHKPAPEALRALLSRDPRA
                     ETE"
     gene            7808..8227
                     /locus_tag="PLP58_07720"
     CDS             7808..8227
                     /locus_tag="PLP58_07720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009960886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="secondary thiamine-phosphate synthase enzyme
                     YjbQ"
                     /protein_id="HRJ08438.1"
                     /translation="MPAHAESFSLATRGKGTYEITGACERIVRASGIRTGTATVFVQH
                     TSCSLVIYENADPSARTDLHGFFDRLVPEDTPWFVHTHEGPDDMPSHLRMALTRTSEV
                     IPVMDGRLALGTWQGIFLFEHRRAPHTRRVVVSVVGE"
     gene            8335..9936
                     /locus_tag="PLP58_07725"
     CDS             8335..9936
                     /locus_tag="PLP58_07725"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase"
                     /protein_id="HRJ08439.1"
                     /translation="MKLLHRLLLPLLATAPLLQGAQTRPNIVFIFSDDHSPTAIGAYE
                     RWLKSVNPTPNIDRLAAQGMLFRRSYCTNSICGPVRAVIETGKHSHLNGFRQNGDKFN
                     WDQQTFPKLLQKAGYQTVLYGKYHLEGKPQGYDDWAVLPGQGLYYNPDFMTAAGKITK
                     EGYCTDLVTGMAVEWLKTKRDPSRPFLLRVQHKAPHRNWMPALRHLGAYDDALIPEPP
                     TLFMDHRHQASPSRHQELEVDRHMDLNYDLFVDLTPDFDQPPSQQRQDRSAWMNMKRM
                     TPAQLSAWRAHYGPKDAEFHKANLSGEALVRWKHQRYMRNYLACVKGVDESVAALMKT
                     LEELDLAENTIVVYSSDQGFYLGDRGWYDKRWMYEPSLEMPLIIKWPGVTTPGTRNDA
                     LVQNLDYAPTFLEAAGAPVPADMQGRSLVPLLKGQRPADWRDAIYYHYYEYPSVHMVA
                     RHRGVRSERFKLIHFYQFGEWEFYDLDADPQELRSQYDNPVYAREVAAMKKRLDELST
                     QYADDSDIAEMPAEWKVKVRQPAAP"
     gene            complement(10029..10646)
                     /locus_tag="PLP58_07730"
     CDS             complement(10029..10646)
                     /locus_tag="PLP58_07730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ08440.1"
                     /translation="MTTRLSWILAAGLCLVALGGWYLGAPTGGRAVPADQTSPPESGS
                     APERLVTVADPAEVFQKAFWKRPAADDTILHAERREWKTEEGVSKWQWFISVRPGHDL
                     VGHLIKENAFMLAKLEEGDILPPQEPPEWFPASKEGCEVFANASKSFRLLWDREKNLL
                     HATDSGGGFQPGAPQPAKPVSVSANTPGRLPLTPPPNPRDEVERE"
     gene            complement(10648..18294)
                     /locus_tag="PLP58_07735"
     CDS             complement(10648..18294)
                     /locus_tag="PLP58_07735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ08441.1"
                     /translation="MNRVLFPFLLLLLAGAAHAQFEIKGGATNLLDQRRAGASATVSG
                     GAVNAITVRGGGGGYPVSPAPVVTIAAPPSGTTATATAVVSGGVVTSITVNTGGSGYS
                     TPPAIFIAPPVTPSGGATTTPQFAGRATTSATAGAISTAGVTAGRYPRAQDVTVPASP
                     VVTIVLARASFGHSFASGVPRYFMGDEITRPNLRWDGQPVAQTYWRSKPVQPGESFDP
                     ALIEGTVTGQTQPLVPTGNVTVTQSSTSSSVATISSVTAGLTAGATLLGQRVNMINGL
                     TVTLAGNASENISSSTSRPFAPYQPFYFSPHANKVFASQAGRVTITWVSNVPDTTKPG
                     ESTPTYKFRQETFAVSSASQSPARVIYWTEKGFDGPIVNIPPGRIVRVNPVFNSFVTG
                     MTDEYVPVGSNPGSIGSASENRTFWFDNENGPPSLHAYNIEGRLFIEYLGSENQGSTG
                     VHQFIGADIVDIRRVAEVETITTHLGEKLRPRQGAPENGDDQLDPVMLFNLIVGGKLP
                     YGSFIRPDGVTDYFAERESLDADRVTIYWMEPNDASIHFQEPPSAPGISVKWPKYKRN
                     YIQRWPDSIAAFEPVNVLQTGAGPAVGPRFVASSLPQVIFQDDPNELEAQLDAATQRL
                     VVDFSDSGDKTNRSLLKFSSSVSPWYVRIYIQSEEALGAPEIPDPDGSGPLTGTPARY
                     TLNDLNADGAPDLNLTANVGDRLEPPHPSYALGGHISKGTCYSEAAYINPLDSAGVEA
                     ADAGGIIPVNALNTDRVLWVWWLKKIVPPSNKYEAFHVPAVAARYTVSYPASPQELVM
                     ASGKGVENPGLTSVQSEGSIYVQNDPSKIGYNPNEEHGLMLAGNAYALRDDLNTASSS
                     EPFVLIQYNEPATETVPARPAMRVLKVVRSNGTYPLVYNKIAGTPVQPPMPLTALPLP
                     LKTDGKVRNTEEEITPDAAAGSGAPDAYDSFTFEDRKGFHWIYRGPHAGGAPSFGMKF
                     YYYLREGFHFPSLGTQPAVGSIQPYIADTGSGEAVTLTYLPKWPDDPTLPPADQKVLD
                     VLFMAETMALQKGTRPQVRGQSSAQVIYQQSIANTGAANPAVALHDPTRQKIALLEAH
                     GLDKLPASILTSDYAGKTYFQRLPPNLQNRFHFNAGLGAKGGLVLVGEFIDEIASEDY
                     FHLNKLSVQDIADLKALCPDGDSANKTKWDNAIAALSTTLETFRESTEVPGTYVRVPG
                     LDKQVTVGQRSDVTDPDTAVDSYALSSTGKGTGYVTLVFGNGEAFTDEGDPVAMEIIK
                     VSNALYKGDLKILLSSNPLDEQVALRHSGDYGGEPENFEFEWRYGFAENGLPPAGDPA
                     TDPDWINPGGTLGSSILIGGSPTAVLSTPAVIMGDVWFTMRYKKTGGAYSDWMSPALV
                     EGWVKRVLAKITPFNQRMDDLFNNSLNTDVSLLTQAGTRWEGNIALNLNNVNEAGLIE
                     IYETVLNRAKDFTIGSGIDFGPSNDALILAAGYLHDLYTILGNEAFADAANPTISIDD
                     QDTITEVNTSRYSFEAQVASSLEEELALLRGRDDFGLPEVGIAPAYNRLWWNYTRGID
                     SGEVLYAVNYNIREKEGGSAANGIVDAADAQHMFPQGHGDAYGHYLTALKGYYKLLTH
                     PQFTWTPRAEAVNILGQPVLIDYQDERKFAAAASNVARTTQQVLALVHRQNYRDDPGA
                     GWAHFRDGKENTRTEVVRHMGLDETASRSAQGAFFNWVVGNALLPDEDTNPSHTGVQI
                     IDRTTVPELDELTGYAESFQTTMDLANAHLNPLGLSPGAIAFDISPAEHQAGKSHYEQ
                     VYERALGTLLNAKGSFDQAAKMTRLLRNQENQIADQQTAIEDQEYAFLNELYDIYGMA
                     YPGDIGPGKTYQQGYEGPDTENWFIIDRPVDLVDTTKPVTVTVRVPTNIRRFTGDAYN
                     DIAESWNTKDTVVKTFKVTPNQVVQFADVVGPGTALGKRPYTGALQEALLEAHLAQVA
                     LLEANHGLQFAVENFLREGEVFRALVQSHKDNLDTRFKYGIASSLNTMAASALNNLGI
                     SLQWAAEAARDAAEAVQEALPKSVGLATDMTAPARGAVKFAGFGVGSGFMVGAIASYA
                     GSSALEGIGAALEADLEIDLEELGFDQDELQAVYEFEQTYREMLSQHHQFVGLMAALQ
                     TANQKVENLRVAGDTILLRRETLRKRAAAVLTGYRTKDLTFRTFRNEALEQYRTLYDL
                     AARYSYLAAKSYDYETGLLGTTQGQAVINRLVASRSLGDLTGGVPQATTSTLGDAGLA
                     GTLAQMNADFAVAEGRLGINNPDPYGTLFSLRHEMFRIVDDPDETDDDEAWQQTLEQH
                     IMPDLMADPHVAKYCNNIKLPGAGATPGILIPFSTVINHTDNFFGLPGAAGDHAYSPS
                     SYATKVSSSGIVFKGYIGMDPYAFGTPNAGGPNTSHPDALRATPYVYFIPCGYDYMLA
                     PPLGDTNTVRAWAVHDQALPLPYNLGANDFNATQFFSANGTLSEQPWILRKHQAFRPV
                     SDPAFFYSTMPAEFTNRRLIGRSVWNSQWKIVIPAYTLLSNEQEGLNRFVRSVKDIQL
                     FLRTYSHSGN"
     gene            complement(18747..20900)
                     /locus_tag="PLP58_07740"
     CDS             complement(18747..20900)
                     /locus_tag="PLP58_07740"
                     /inference="COORDINATES: protein motif:HMM:NF025253.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber domain-containing protein"
                     /protein_id="HRJ08442.1"
                     /translation="MKTHRIHLILAAFCALPGLLAAQTITTGVPGFISYQGKVLNNTG
                     GLVGSPTPVNRTVIFRVWDHPSNTLTGNLIYSEQQTVTIADGEFSVLVGQGVATTGSQ
                     FGYSETAKKLADIATAFGGSTRYLGVTVDDGTAAVDNEITPRQQIVSSAFSFRAKFAE
                     NLGTSASTALTTVDSGNVGVGNTNPTARFMVTAAGTGTGSPQMIVTADDTTERLRIGV
                     DSTGTGTGYIQSFKEGIGAQNLLLNPSGGNVGIGTTSPSHKLHVFGNTALQGSASSDS
                     VGFSIYNSGGAERATLGMAGAGGAYSSDAVQGDTIVRAFNGKLLLQSGTGSSAICINS
                     SNNVGIGKSNPGSKLDVNGNISATGNLSVTGTNGSFSTPFFPPSGTTTLSLFVAGVGA
                     NEPQMRFNRTSDGQFYDIGMNGSNGFVIDDNDTPRLTINSAGNVGIGESNPTARLHVN
                     GAIQVANDNNITGLNALIGFNDLHLQGDSSAVDLSIHADGEVTVGGTRIYNNVTFQVK
                     GIPGDDLVAMFRNPSGADAYYFWNNGAAEKIGGTSWTALSDQRVKKNIVEVEGSLEKL
                     LKLRSVSFEYIDPVKHGEGKYNGFIAQEVEAVYPDWVTKGMDGMKRLTINGFESLAVQ
                     AFRELRQEKNAQISKLEEANKELQARLKKLESSNSDSASAGLSNAELLERLALLEARD
                     KAREAKLAAIEKLLLENGQPAAQPVSLKKAANDAE"
     gene            complement(21039..22748)
                     /locus_tag="PLP58_07745"
     CDS             complement(21039..22748)
                     /locus_tag="PLP58_07745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ08443.1"
                     /translation="MSVHQWFFLFFIIGATAQAQWQSTTYALKGGYNSIYLHGAATHA
                     TMEQLFPNSGDTANVQEVWRWNPNPTQVQFTTTPLLPTQGTPEWSVWVRGGGANTLPN
                     LTGPAAYLVKCAGTASNTYNVPIVHKPAPPNSTWVRNGANFLGFPSRLNGNYPFFSTY
                     FATFPAAIATNSKIYKYVGGDLSASNPIQVFSPSAERLDRNQAYWFESEVVGNFYAPV
                     HVTLSLTGGLDFGRNGSIVTARVLNRTASVMTLTIAPVASNAAPAGQEAITGPVPLTR
                     RTFNTGTAAWEETLIATAYNEVIAPQSAVELSFGIDRVAMGGTADALFASMLRLTDAA
                     NLFDILIPATARKTSLAGLWIGEARVTAVESKAQADAITPASRPFPLRYLLHVADNGT
                     ARVLSQVFLGQLAAAPHDFGICTKEAGLKADAKDKAARIVATHMPLDRVLTAGTGSVA
                     IPGTLTRTITVPFDDPVNPFVHQYHPDHDNKSPTGAALIAGKESHAVTREVTFTFTTA
                     PPEGSTATGGWGSAVIGGTYAEVIHGLHKDSAGVGTGDGLHLTGVFELRRVSELGAIS
                     VTP"
     gene            complement(22867..23709)
                     /locus_tag="PLP58_07750"
     CDS             complement(22867..23709)
                     /locus_tag="PLP58_07750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ08444.1"
                     /translation="MNTAVRFFCVSCIATGGLMAFPPAPYYTIYGIVRDQVGQTLAVE
                     GAQIILLKGQTEVGRAPVVTGLQLDQNYEISLRIDTGRPGTALYTSAAIQANTPYSLA
                     VEMNGERFYPIEVAGTLTAGKGGERVRLDLNLGEDSDGDGLPDIWEQWQLYQAGHYPD
                     GNGVWPIHLIDRDGDFDGDGMSNWFEYVAGTFAGDATERIDMQIKEKTASFVRLEFFA
                     ITGKVYTIERSLNMQGWETVPFSFTAEGTPQQVFVAEDVGILPAFTIPTPGGAREFYR
                     LTVR"
     gene            23866..24609
                     /locus_tag="PLP58_07755"
     CDS             23866..24609
                     /locus_tag="PLP58_07755"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HRJ08445.1"
                     /translation="MKKKNPPAPEASAFPTTHWTLVRTVQGKDAAAAARAMEELCKGY
                     WYPIYAYLRRSGHNPEDAEDLTQSFFEKLITEDAIQTAREEAGKLRSFLLGILRHALS
                     DHLRNTGAQKRGSHSPHVSFDEMNAEQRYASEPQDIRDPEWIFTRAWAHELLAGVREK
                     MRAAFKSAGRGETFDLLLPYLLWDDEPPSHRELAEKLGSSEAATRILIHRLRTKFREL
                     LRTEVARTVLTPEEIPGELAWLQGVLAEK"
     gene            24680..>25543
                     /locus_tag="PLP58_07760"
     CDS             24680..>25543
                     /locus_tag="PLP58_07760"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HRJ08446.1"
                     /translation="MTPASSSSKPAGPWQPPSLEEMQAMLPQYHFLSLLGRGGMGAVY
                     KAVQTSLDRVVALKVLPGALSAGDDAGAEFAGRFKNEARTMARMRHPGIVNVWDFGQT
                     GTGMFYIVMEYMDGTDAAQLIRSQGRLPEAHALSITAHVCDALAYAHAQGVIHRDIKP
                     ANILIKQTGEVKVADFGLAKRSDPALSGLTKTNMAMGTPDFLAPEALIPGIPLDGRAD
                     LYAVGVMLYQMLTGELPRGMWEMPGARLGTDPRFDEIITRAMQADREARYQSAAEIRR
                     ALDTILTRPKPA"
BASE COUNT         4483 a         8135 c         8371 g         4554 t
ORIGIN      
        1 cgagcttgcg gatgatgtcc ttgcgtgtgg tgacaccgtc tttcacgccg gcggcggctt
       61 tgctcaaatg ggtgggctgg gctgcggaag gatccgtgat cccgcttgaa gcggatgagg
      121 cagccggagc tgtggtgctg gccgcggtgt cagcagcggg ggcgctttct ggtgtggaag
      181 tgccggcgca gtgagtcagt gagaggatgc cggcggcggc ggcggaacga atcagggtga
      241 tgtttttcat gcgctgtgaa tcaagcccgc gaatgccgcg cggcaaggct gtcctggagc
      301 ggattgtcat cgcggcctgg cgcaacttca cacctggctt gggttttcac cgcggtcatg
      361 aaaatcccgc tctttcccgc gctcgccgtt gtctctgtca tcgcccatgc cgcgaacgcc
      421 cagggaccaa acacgcgcgt tcccgaaggc gtgcgcgctc accgcgacat cgtttatgtc
      481 gagggcgggc atgagcggca caaactggac ctgtatctgc cggagaagtc tcaggggccg
      541 ctgccgctca tcatttgggt ccatggcgga ggctggatga acggcagcaa ggatggctgc
      601 ccgccactgc gcgccggtta tgtggaaagc ggctacgcgg tggcctccat caactaccgc
      661 ctcagcagtc acgctgtttt ccccgcgcaa atcgaggact gcaaggcggc catccgctgg
      721 ctccgcgccc aggcgggaaa atacggcctc gacgccgggc gcttcggcgt ctggggcagc
      781 tcggcgggcg ggcatctggc ggcgctgctg ggcacgagcg gggatgcgcg ggagttcgac
      841 accggcgcgc atctggatca aagcagccgt gtgcaggccg tttgcgatta ctacgggccg
      901 acggacttca ccgtcttcgt caccacgccg ggctatgaaa gccacgcctc ggacacctcg
      961 cctgaggcca ggctgctcgg cggcgcggtg atgcggaaca aggataaagc agcgcgcgcg
     1021 aaccccatca cccacatcag caacgacgat ccgccgtttc tcatcgtgca tggcgaccag
     1081 gaccccaccg tgcccatcaa tcaaagccag cttcttttcc gcgaactggg ggccgcggga
     1141 gtcaacgcgc acttccacac catcaaagga gcgggtcatg gaggacccgg attcagcggc
     1201 gaggacatcg gccagatggt ggccgctttt ttcaacgccc ggctcaaaga tggcatcagc
     1261 ggcacgggaa cgctcgccac ccagagcacg atcgcgccag tcggcggccc gcgcgccggg
     1321 cgcgatggcg tgcgaggccg gtccgctccg gagccgcggg aggcatccac cgccaagggc
     1381 ttcaagctcg aaggagagca ttggacttac cgcgaaggtg acctcaccat gagcggcatt
     1441 cttttaaaac ccgagggcaa ggggccattc cccgcagtgc tcatcagcca cggcctcggc
     1501 ggcagcgcgc gcagcttcgg cctcaccaaa gcacgcgaga tggtgcgctg gggcatggtc
     1561 tgcatcgcgc cggactacac tcacaccggg gcgggtggcg accgcgtcca gttcggcgcc
     1621 agcgcggaga atgtgcggcg cgccagcacc tgcctggaaa tcctgcgcgg catgccggat
     1681 gtggatggcg cgcgcctggc cgcctacggg catagcatgg gcggctttgt caccatcggt
     1741 cttgcagcct ccgcgcccgg gctgctcaaa gcggcggcca tcaccggcag cggcatctct
     1801 ccgcaggagg gccacccagc gccctcgact tccacggcgg agaaggtgcg cacgcctttc
     1861 ctcatgctcc atggcgcgaa cgacaccacc gtgcgccctg aacagtccgc ctcgctcaag
     1921 caggtgctgg acagcaacgg cgtggtcaat gaccgcctca tcgccgacgg ccagggccat
     1981 ccgatagacc agaccatgcg tgaggaggtc ttccgcctca ttcgtgaatg gttcatcaag
     2041 aacggagcgc tcggctccaa gtccaaataa gccgccactg cgagcagcct cgcagccgct
     2101 ctccagagtg cggttgaatc cacggccccg ccgcgcagtc ttaacgcccg ccatgatgaa
     2161 ccgccgcatt ttcctagcca gcctcgctgc cgcacctctc gcacgcgccc aggatccgac
     2221 acccaccgcc gaggaggtgc tcaggctggt gcgtaaaagc tacgcgctgc aagaccacaa
     2281 aatgacaggc cgccttcgtg atggaaactc gggacgcgag gagccgctgg agctgacgct
     2341 gacacaatcc gtgatgcgtt tccgcttcat gaacccgcct gcggagatcg tccatctgga
     2401 cctgaccact gagcccgcca cactctggga ggtgaagacc ggcggcagca gcaaggtgcc
     2461 tttgtccaac gcctccaacg cggtgcgcgg catggacttc aattacgagg acctttccca
     2521 gcgcttcctc tactggaaaa acgccaagat gatggacgcc aacgcgcgtc tcagcgcggc
     2581 gggcatcaaa tgctggctcg tccgcgtcac cgcccccgac actaaaggcc cctactacac
     2641 cgtggacctc tgggtccaca aggacagcgg cggcgtggcg aagatggagg cctacaatgc
     2701 cccgtcgaaa ctggtgaagc gcttcgaggt caccaaaatg tggaaggtgg gcgaggccac
     2761 cgccctgcgc gaaatgcgcg tgcagtcctt caacccgctc gacggctcgc gcaagggcac
     2821 cacctacatg acgatggaca agccggagaa acagtaggtg attctaaacc gtggttgcgg
     2881 cgactcgcga tgacgacact caccatgcct tggcgcccgc tccgataagt tcgccggctt
     2941 catcttcggc ctcttcctct tcagtctcca ggggggcgaa gcgctcggag cggaaaccga
     3001 gctcttgttt gacgctggaa tgcggatcgt cggggttttg cagctcctcc aggaggatga
     3061 ggatgtcgaa ctcggcgccg gtgagcttga tttcggcgtc gtcgttgagc gagaacacag
     3121 ggttcgagcg tccggagcgg acggcgcgga ccgtgtaggc gcggcctttg cgaggcaggg
     3181 cgcggtaaag atcatagacc caggggtcga attgatcgtt gatgcagacg actttctggc
     3241 cggtttgaaa catgggtggc gattctggcg cggagtggcg gcggggtcaa atcctgccac
     3301 tctcgcgcag gtgctggcgg aggcgtttga ggacttcggc gatgacttcg ggctgctcca
     3361 cgcagccgtg ccagtggggc acgacccgct cgctgccggt gggcaggtgc gcgctgcggt
     3421 agggcacgac gaggtcgctg ctatcctcga gggttctgcc gcccacgcgg ccgatgatgg
     3481 agtgatgggg cacgggaatg ggcctggcgt tgagcgcctg gatatagggg tgcctggggg
     3541 aaagagtgct gaggctgaga aaggagaaaa caccccagtc gcggatttgc ggctggaggg
     3601 cgtcggtgtt gccggaaagc agctcggtga cgacgagcat ggagtccacc ggcagccgga
     3661 tgagcttggc caggcgcatg acgatgcctt tgtccgccag gtcgctgccg cgctggggtg
     3721 tggcgatgaa gatgaggcgc gccacttcgg gacgtttttc aaagatgaag ccggtgcgca
     3781 gacgctcgcg gcccgactcg gagaggcgca tctcctccgg cggcttggtg aagtgggcgt
     3841 tccaaaattc catgccgctg tccaccgcct gcatccggct cagcaggccg cccatgctgt
     3901 ggcccacgag gaccatttga ttcatcgccg ccgaggtgcc gcccggatca agccggtcgc
     3961 gggcttcggc gatggactcg cgcaggcggc gcgaggtggc gggcacatac atgccggtgg
     4021 ggtagaggaa ataccagggc tggaaatgcg cgcggagcac gggatccgcg cagagcgcgt
     4081 tgatgcaggg ataccagatg tgcgggtcgg acatcaaccc gtggacgaaa acgacgggga
     4141 tgcgccgcgg atcatacggg tcgagctggt aaagacggct gtcatcgagc gtcttctccg
     4201 ggtagagcag cccggtgagg gagaattttt tcaggaagcc cgcgtcgagg gccagggcct
     4261 tgggcgcggt gtagttggcg gccagaggcg tgtctttgcc ctcaatgcgc acacggggct
     4321 gctgaagggg attcacgaga cgcaggcggc actcgcgcgg ctggccgggg acgcttttct
     4381 caaactcgag cagggcggtc agcgtgagat gcgcgccgtt gcgcggcagg agagggtgct
     4441 ggtcgctctc aaaaacacgg cccaccaggg gcacaccgag accgtcagtt tcagcgaggc
     4501 tgccggcttt gccggtgcgc acctcgctgg cgaggcggag gtccgccagg acgcgcggcg
     4561 tgatctcgcg cgtgtcgtga ttgcgcgtct gaatgcggac cctgtgggcg gcaaatgccg
     4621 cgccgtcctg ccagcgccgc ggcgcctggg cgcggctcca gcctttgaga aaggcggcga
     4681 gggccttttc gtacctctcc tcggcctcgg cgcgctcgcg acggttcgag cccggcgaat
     4741 gcagccgctc ccaggccgcg ccggcctcgg ccaggtgtcc gccgagcctc gcgcgcgcgg
     4801 attgtgtgaa gcgccattgc aggggctcgc gcgcttccgg cgtcttcggc gggccgatgc
     4861 aggaggcggt ggacagcaca agtgccagca gcagcaggcg agtcatgact gcggaggttt
     4921 ggcggggtgc tccgcctcgt atttccggct gtagcggaca aaggcgaaga aggctgtggc
     4981 cgcggcgatc cacaggccgg ggaagccatc gagaaaaccg cgccgcagca cataggcgcg
     5041 gaagaagcgc cagagcgggc gtgtgagcgt gggcagcagc gcccagcgca cgccgtcggc
     5101 ctcctggcgc tggagaaaga catcggcgaa gacattgatc ttcgagatgt agtggctcat
     5161 gtcgcggaag gagtagtggt gcaggtcgcc atccaaaatg aggcagggcc gcgccccctc
     5221 cggcagcacc actttgtcat gctccgggct gccgccccag agggcgcccg cgcgcttgaa
     5281 gaggcgcagc ttgcggtcgg gataccagtc gccgtgggtg atccagcggc cgaggaaaaa
     5341 caccttgcgc ggcatgatgg cgccgtcgtt ttgctcgtgc cccccctggg cgaagaagtc
     5401 ctgaatggag cgcctcagct ccggcgagag ctcctcgtca cagtcgagcg ccagcaccca
     5461 gggctgggtg cagaggcgga gggccacgtt tttttggtcg cgatggccga gccagtcctg
     5521 atgctcgacc cgcgcgccga actcccgcgc cacggcgcgg gtggcgtccg tggagccgga
     5581 gtccaccacg atgatttccg ccgcaaggtc cgccgcgctc tccaggcagc ggcgcagatt
     5641 ggcctcctcg tttttcgaga tgatgctgat tgagagcggc aggggcatgg aagcgtcacc
     5701 ttggcccgcg ccgccggcgg gtccatttca aaaagcgtgg cggatcactg aaccttcatg
     5761 accccgttca tcagcacggc atggccgggg aaggtgcaaa ggtaggggta gtcaccggcg
     5821 gctggcgcgg tgaactcgag gacctcgctt tgattgggct ggagcagctt ggtgtgaaaa
     5881 agcacttcgg gcgcctcggg gatgaaaccc ttggccatcc cggcggggtc ggtggccatc
     5941 tgcatggcga gacccatgag cttgtccttc gagccgaggg ctccgatgac aatgttgtgc
     6001 ggctgcggca cggtggggtg gacattgttg aaggtcagct tcaccttggc gccagccttg
     6061 acggtgaatt ccttcgtgtc gtaggccagc ggattggtga ggtccggctt gatggtgatc
     6121 tcgatgacct cgcccgtggg agcgggaacc gcaggcgcgg tcgcggcggc ggcgggtgcg
     6181 gaaggagtgg cggcggaagc agcaggagct gccggcgttg cgggggcgct ggcagcagtc
     6241 tctgccccgg cctccgtcga gccggtggtc tggctgacga cgccggtcaa aaacgcccct
     6301 gcccagaaca ggacaagacc gccaagggtg acggcgatca ggacattgag gatgctccag
     6361 atggagccgg actcttcgga tttgacgggt gcggacatgg cgggggggat ttttggagcg
     6421 ccgaaacaag cacaggatgc agtgaacgca agcccgcacc tccccgcaga cggagcgcca
     6481 gaccgaaacg gcgccacata tcaaacaccc gccggctgtc tctccctgga ggggaaaatg
     6541 gcggcttttt ggtgttcctc ccgcggaacc agcgccaaac gacgaccgtt tggcgtgcaa
     6601 aagtactttg gaacaccaaa cgacggtcgt ttggcaccgc cgaccgctcg ggggggccgt
     6661 caatgccgtc actgaagtcc atgccgtcca tgcagtccca ccccggcagc acggatgccg
     6721 gcgcttgcat caccgggccg gaagtgccaa caaacggcat cccccacgca gcccacgccc
     6781 cacccatgcc aacgcccgac ctcccccgcg ccatcgtcct cggagccgga agctggggca
     6841 ccgccctggc ggccatgctg ggcatgcgcg gtgtccgcac ccagttctgg gggcgtgatg
     6901 ccgggctcat gcgggaaatg cgcgagacac ggcgcaacgc gcgctacctg cccgcgctcg
     6961 aattgccgga gagcgtcagc gtcgccacct ccctggccgg cctggagtct gcggagatgc
     7021 tcgtctttgt caccccgtcc aaaggcgtgc gggagacggc gcggctcgtg gcggagagcg
     7081 gcctggcggc ggaggcgcgc gtcattctct cctgcgccaa aggcatcgag ctggacagcg
     7141 gcaaacgcat gagccagatc ctcgccgagc atttccccgg caaggcgctg gccgttctca
     7201 cggggccgaa ccacgccgag gaggtggccc agcagctcgc caccgccgcc gtcgtcgcct
     7261 gcgaggacga ggccgtggcg cgcgccgtgc agagctgcct gacgctgccg tttttccgca
     7321 cttacaccag tgatgacgtg accggcgtgg agtgggccgg cgccatgaag aacccctacg
     7381 ccatcgccgc cggagtcgcc ctcggcctgc ggctggggga caatgccatc gccgcgctcg
     7441 tcacccgcgc gctggccgag atggtgcgcc tcggcaccgc catgggcggc agagtcgagt
     7501 ccttctacgg cctcgccggc gtgggggatc tcatgggcac ctgctactcc gagcacagcc
     7561 gcaaccaccg cctcggccgc cagctcgggg aggggcgccc gctggcggaa atcctcggca
     7621 gctcgcgcat ggtcaccgaa ggagtgccga acaccgccag cctgcaccag gccgccaagg
     7681 ccgccggcgt gcgcaccccc ctgctcgacg aggtccacgc catgctttac gaacacaaac
     7741 ccgcgcccga ggccctgcgc gccctgctct cccgcgaccc gcgcgccgaa accgagtgac
     7801 ccccgccatg cccgcgcacg ccgaaagctt cagcctcgcc acccgcggca agggcaccta
     7861 cgagatcacc ggcgcgtgcg agcgcatcgt gcgcgcctcc ggcatccgca ccggcaccgc
     7921 caccgtcttc gtgcaacaca ccagttgcag cctggttatt tatgaaaacg ccgacccctc
     7981 cgcgcgcacg gacctgcacg gcttcttcga ccgcctcgtg cccgaggaca cgccctggtt
     8041 cgtccacacc cacgagggac ccgacgacat gcccagccac ctgcgcatgg ccctcacccg
     8101 caccagcgag gtcatccccg tcatggacgg ccggctcgcg ctcggcacct ggcagggcat
     8161 cttcctcttc gagcaccgcc gcgccccgca cacccgacgc gtcgtcgtca gcgtcgtggg
     8221 agagtgaggt tggagtgaag tgttggagtt gaagcattca ggcgacacgg cagcgcgcgc
     8281 cttggaggca atccgcgctt gcccctgcgg catcacgctt catagaaaaa cacgatgaag
     8341 ctcctccacc gcctgctcct gcccctgctc gccaccgccc cgctccttca aggcgcgcag
     8401 acccggccca acatcgtctt cattttctcc gacgaccact cccccaccgc catcggcgcc
     8461 tacgagcgct ggctcaaatc ggtgaacccc acgccgaaca ttgaccgcct ggccgcgcag
     8521 ggcatgcttt tccgccgcag ctactgcacc aactccatct gcggccccgt gcgcgccgtc
     8581 atcgagaccg gcaagcacag ccacctcaac ggcttccgcc agaacggcga caaattcaac
     8641 tgggaccagc agacctttcc caagctgctg caaaaggccg gctaccagac cgtgctctac
     8701 ggcaagtatc acctggaagg caaaccccag ggctacgacg actgggccgt gctgcccggc
     8761 cagggcctgt attacaaccc cgacttcatg accgccgccg ggaagatcac caaagaaggc
     8821 tactgcaccg acctcgtcac cggcatggcc gtggagtggc tcaagaccaa gcgcgatccc
     8881 tcccgcccct tcctcctgcg cgtgcagcac aaagccccgc accggaactg gatgcccgcc
     8941 ctgcgccacc tcggcgcgta tgacgacgcg ctcatccccg agccgcccac cctgttcatg
     9001 gaccaccgcc accaggcctc gccctcccgc caccaggagc tggaggtgga ccgccacatg
     9061 gacctgaact acgacctctt cgtggacctc acgccggact tcgaccagcc gcccagccag
     9121 cagcgccagg accgctccgc ctggatgaac atgaagcgca tgacccccgc ccagctctcc
     9181 gcctggcgcg cccactacgg cccgaaggac gcagaatttc acaaagccaa cctcagcggc
     9241 gaggcgctcg tgcgctggaa gcaccagcgc tacatgcgca actacctcgc ctgcgtcaaa
     9301 ggcgtggacg aaagcgtggc cgccctcatg aaaacgctgg aggagctgga tctcgcggaa
     9361 aacaccatcg tcgtctattc ctccgaccag ggcttttacc tcggggaccg cggctggtat
     9421 gacaaacgct ggatgtacga accctccctg gagatgccgc tcatcatcaa atggcccggc
     9481 gtcaccaccc ccggaacgcg caatgacgcc ctcgtgcaaa acctcgacta cgcccccacc
     9541 ttcctcgagg ccgccggcgc gcccgtaccc gccgacatgc agggccgctc cctcgtcccc
     9601 ctcctgaaag gtcagcgccc cgcggactgg cgcgacgcca tctactacca ctattacgaa
     9661 taccccagcg tccacatggt ggcccggcac cgcggcgtgc gctcggagcg cttcaagctc
     9721 atccatttct accagttcgg ggaatgggag ttctacgatc tcgacgccga cccgcaggag
     9781 ctgcgcagcc agtatgacaa ccccgtctat gcgcgcgagg tggcggccat gaaaaagcgt
     9841 ctcgacgagc tttccaccca gtatgccgac gacagcgaca tcgccgagat gcccgccgag
     9901 tggaaagtaa aggtgcgcca gcccgccgcg ccgtgagggc ttgctggaag cccataccga
     9961 ggcataaacg gcaagaaact gcccgcacgg gagactcctc ccggccttcg tttgcccaat
    10021 cggcgggatc actcccgctc cacctcgtcc cgcgggttgg gaggcggggt gagaggcagg
    10081 cgccccggtg tgttggctga cacggacaca ggcttcgccg gttgcggcgc gccgggttga
    10141 aaaccgccgc cgctgtccgt ggcatgcaga agattttttt cgcggtccca gagcaggcgg
    10201 aaggacttgg aggcgtttgc aaaaacctca cagccttcct ttgaagcggg aaaccactcc
    10261 ggcggctctt gcggtggcaa aatgtcgccc tcctccagtt tggctagcat gaaggcgttc
    10321 tccttgatca aatgccccac caagtcgtgg ccgggccgca cgctgatgaa ccactgccat
    10381 ttcgagacgc cttcctccgt tttccactcg cgccgctcgg catgaaggat ggtgtcatca
    10441 gcggcgggac gcttccagaa agctttctga aaaacctcgg cggggtccgc cacagtgaca
    10501 agccgctccg gcgcggagcc tgactctggc gggcttgttt gatctgccgg cacggcgcgc
    10561 ccgcctgtgg gcgcgccaag ataccagccc cccagggcca caagacaaag ccccgccgcc
    10621 agaatccagg acaagcgtgt tgtcatgtca gttccccgag tgcgagtagg tgcgcaggaa
    10681 gagctggatg tccttcaccg aacgcacgaa gcggttcagg ccctcctgct cattgctcag
    10741 caaagtgtag gcggggatga cgatcttcca ctggctgttc cagacgctgc ggccgatgag
    10801 gcggcggttg gtgaactcgg cgggcatggt gctgtagaag aaggcgggat cgctcaccgg
    10861 acggaaggcc tggtgcttgc gcaggatcca gggctgctcg ctaagggtgc cgttggcgct
    10921 gaagaactgg gtggcgttga agtcgttggc acccaggttg tagggcagcg gcagggcctg
    10981 gtcatgcacg gcccaggcgc gcacggtgtt cgtgtctccc aggggcgggg ccagcatgta
    11041 gtcgtagcca caggggatga agtaaacata cggggtggcg cgcagggcgt ccgggtggct
    11101 ggtgttcggg ccgccggcgt tcggggtgcc gaaggcgtag gggtccatgc cgatgtagcc
    11161 tttgaagacg atgccggagc tgctgacctt ggtggcgtag ctgctggggg agtaggcgtg
    11221 gtcccccgcc gcgccgggca ggccgaagaa gttgtcggtg tggttgatga cggtgctgaa
    11281 ggggatgagg atgccgggcg tggcgccggc gccgggcagc ttgatgttgt tgcaatactt
    11341 cgccacatgc gggtcggcca tgaggtcggg catgatgtgc tgctccaggg tctgctgcca
    11401 ggcctcgtca tcgtcggtct catccggatc atccacgatg cggaacatct cgtgccgcag
    11461 actgaagagg gtgccgtagg ggtcggggtt gttgatgccg aggcggccct cggccacggc
    11521 gaagtcggcg ttcatctgcg ccagtgtgcc ggcgaggccg gcatccccga gggtgctggt
    11581 ggtggcctgg ggcacgccgc cggtgagatc gccaagggag cgggaggcga cgaggcggtt
    11641 gatgacggcc tggccctggg tggtgccgag caggccggtc tcgtaatcgt agctcttggc
    11701 cgccaggtag gaatagcgcg cggccaggtc ataaagcgtg cggtattgct ccagagcctc
    11761 attgcggaag gtgcggaagg tgaggtcctt cgtgcggtag ccggtgagca cggcggcggc
    11821 gcgcttgcgc agagtctcgc ggcgcaggag gatggtgtct ccggcgacgc gcaggttttc
    11881 caccttctgg ttggcggtct gcaaggccgc catcaggccg acgaactggt gatgctggct
    11941 gagcatctcc cgataggtct gctcgaactc atagaccgcc tgcagctcgt cctggtcgaa
    12001 gcccagctcc tcaaggtcaa tctccagatc ggcctccaag gcggcgccga tgccctccag
    12061 cgcgctgctt cccgcatatg aggcaatggc acccaccatg aatccactgc cgacaccaaa
    12121 gcccgcgaac ttgaccgcgc cgcgcgcggg cgctgtcatg tcagttgcaa gacccaccga
    12181 ctttggcagg gcctcctgca ccgcctcagc cgcgtcgcgg gcggcctcgg cggcccattg
    12241 gagggagatg cccaggttgt tgagcgcgga tgcggccatg gtgttcaggc tgctggcaat
    12301 cccgtattta aagcgcgtgt ccaggttgtc tttgtggctt tggacaagag cgcggaacac
    12361 ctcgccctcg cgcaggaagt tctccaccgc gaattgcagc ccgtggttgg cctccagcag
    12421 ggccacctgc gccaggtggg cctcgagcaa agcctcctgc agcgctccgg tgtaggggcg
    12481 cttgccaagc gcggtgcccg gccccaccac atcggcgaac tggaccacct gattgggagt
    12541 cactttgaaa gtcttcacca cggtgtcctt ggtgttccag ctctcggcga tgtcattgta
    12601 agcatcgccg gtgaacctgc ggatgttggt cgggacacgg acggtgacgg tgacaggctt
    12661 ggtggtgtcc acaaggtcca ccggacggtc aatgatgaac cagttttccg tgtcagggcc
    12721 ctcatatccc tgctggtaag ttttgcccgg gccgatgtcg ccggggtagg ccatgccgta
    12781 gatgtcgtag agctcgttga ggaaggcgta ctcctggtcc tcaatggccg tctgctggtc
    12841 ggcgatctgg ttctcctggt tgcgcagcag gcgggtcatt ttcgccgcct ggtcgaagga
    12901 gcctttggcg ttcaggagcg ttcccagggc gcgctcatag acctgctcgt aatgcgactt
    12961 gccggcctgg tgctcggcgg gggagatgtc gaaggcgatg gcgccagggc tgagaccgag
    13021 cgggttgagg tgcgcgttgg ccaggtccat cgtcgtctgg aagctctcgg cgtaacccgt
    13081 cagctcgtcg agttccggca ccgtggtgcg gtcaatgatc tgcacgccgg tgtggctggg
    13141 gttcgtgtcc tcgtcaggaa ggagggcatt gcccaccacc cagttgaaaa acgcgccctg
    13201 cgccgagcgg ctggcggtct catccaatcc catatgccga accacttcgg tgcgggtgtt
    13261 ctccttgccg tcacggaaat gcgcccagcc cgcccctgga tcatcccggt aattttgccg
    13321 gtgaaccaag gccagcacct gctgcgtggt gcgcgccacg ttcgaggcgg cggcggcaaa
    13381 cttgcgctcg tcctggtagt caatgagcac cggctgccca aggatgttca cggcctccgc
    13441 tcggggagtc caggtgaact gcgggtgggt gagcagcttg tagtagcctt tgagggcggt
    13501 gaggtagtgg ccgtaggcgt cgccgtgccc ctgggggaac atgtgctgcg cgtcggcggc
    13561 gtccacgatg ccattggccg cggaaccgcc ctctttctcg cggatgttgt agttcaccgc
    13621 gtaaagcacc tcgccgctgt ctatgccgcg cgtgtagttc caccacaggc ggttgtatgc
    13681 cggggcgatg cccacctccg gcaggccgaa gtcgtcccgc ccacggagca gcgccagctc
    13741 ctcctccagc gagctggcca cctgcgcctc gaaggagtag cggctggtgt tgacctcggt
    13801 gatggtgtcc tggtcgtcta tggagatggt cggattcgcg gcgtcggcga aagcttcgtt
    13861 gccgaggatg gtgtagaggt cgtgcaggta gccggcggcc aggatgagcg cgtcgttgga
    13921 cgggccgaaa tcaatgcccg agccgatggt gaaatccttg gcccggttga gcacggtctc
    13981 gtaaatctcg atcaaaccgg cctcgttcac attgttcaag ttcagcgcga tgttcccctc
    14041 ccagcgcgtg cccgcctggg tgagcaggct gacatcggtg ttgagcgagt tgttaaagag
    14101 gtcgtccatg cgctggttga aaggggtgat tttggccagc acgcgcttca cccagccctc
    14161 gaccagcgcg ggggacatcc agtccgagta ggctcccccg gttttcttgt agcgcatggt
    14221 gaaccagaca tcccccatga tcaccgccgg cgtgctgagc acggcggtgg ggctgccgcc
    14281 tatgagaatg gaactgccca gcgtgccgcc ggggttgatc cagtccggat cggtggccgg
    14341 atctccggct ggaggcaggc cattttccgc aaaaccgtag cgccactcga actcgaagtt
    14401 ctccggctcg ccgccgtagt cgccgctgtg gcggagggcg acttgctcat caaggggatt
    14461 gctggagagc aggatcttca aatcgccctt gtacagcgcg ttgctgactt tgatgatctc
    14521 catcgccacc gggtcgccct catccgtgaa tgcctcgcca ttgccgaaaa caagcgtcac
    14581 atagcccgtg cccttgcccg tggagctgag cgcgtagctg tccacggcgg tgtcgggatc
    14641 ggtgacatcc gagcgctggc cgaccgttac ctgtttgtcg aggccgggaa cgcgaacgta
    14701 tgtgccaggc acctccgtgc tctcgcggaa ggtttccaag gtggtgctca gagcggcgat
    14761 ggcattgtcc cacttcgttt tgttggcgct gtcgccatca gggcaaagcg ccttgaggtc
    14821 ggcgatgtcc tggacgctga gcttgttcag gtggaagtaa tcctcgctgg cgatctcatc
    14881 aatgaactcg cccaccagca ccaggccgcc cttggctccc agaccggcat tgaaatggaa
    14941 acggttttgc aggttcggag gcagcctttg aaagtaggtc ttgcccgcgt aatccgaggt
    15001 gagaatggaa gcgggcagct tgtccagtcc gtgcgcctcc agcagggcga tcttctggcg
    15061 cgtggggtca tgcaaggcca cggcaggatt ggcggcgccc gtgttggcga tggactgctg
    15121 gtagatgacc tgcgcgctgc tctggccgcg cacctgcggc cgggtgcctt tttgcagggc
    15181 catggtttcc gccatgaaca gaacatcaag aaccttctgg tccgcaggcg gcagcgtggg
    15241 gtcgtccggc cacttgggca gataggtcaa agtcaccgcc tcgccactgc cggtgtcggc
    15301 gatgtaaggc tggatgctgc ccaccgcggg ctgggtgccc aaagagggga aatgaaagcc
    15361 ctcccgcagg taataataaa acttcatgcc aaagctgggc gcgccgccgg catgcgggcc
    15421 gcggtagatc cagtggaagc ccttgcggtc ctcgaaggtg aagctgtcat aagcatcggg
    15481 agcgcccgag cctgcggcgg cgtccggcgt gatttcctcc tctgtgttgc gcaccttgcc
    15541 atccgttttc aagggcagcg gcagcgcggt caaaggcatc ggcggctgca cgggcgtgcc
    15601 ggcgatcttg ttgtaaacca gcggataggt gccattggag cggacgactt tcagcacacg
    15661 catggccggg cgggcgggca cggtctcggt ggcgggctcg ttgtattgga tgagcacaaa
    15721 cggctcggag ctggaggcgg tgttcaagtc atcgcgcagc gcataggcat tgcccgccag
    15781 catgaggccg tgctcctcgt tcgggttgta gccgatcttc gagggatcgt tctgcacata
    15841 gatgctgcct tcggactgaa ccgaggtcag gccggggttt tccacccctt tgcctgaggc
    15901 catgaccagc tcctgcggac ttgcggggta actgacagta tagcgcgccg ccacggcggg
    15961 cacatggaag gcctcatact tgttgctcgg tggcacgatc tttttcagcc accagaccca
    16021 gagcacccgg tccgtgttca gcgcattcac cggaatgatg ccgcccgcgt cagccgcctc
    16081 cacgccggcg ctgtcgagcg gattgatgta ggccgcctcg gagtagcatg tgcctttgga
    16141 gatgtggcct cccagcgcgt aggacgggtg cggcggctcc agcctgtcac cgacgttcgc
    16201 ggtcaggtta aggtcaggcg cgccgtcagc attgagatcg ttgagcgtgt atctcgcagg
    16261 cgtgcctgtg agcggaccgg agccgtccgg atcggggatt tccggcgcgc caagcgcctc
    16321 ctcggactga atgtagatcc gcacatacca ggggctgacc gaggatgaga acttcaggag
    16381 cgagcggttg gttttgtcgc cgctgtcgct gaaatccacg actaggcgct gggtggccgc
    16441 gtccagctgg gcttccaatt cgttcgggtc gtcctgaaag atcacctgcg gcaggctgga
    16501 tgccacgaag cgcgggccga cggccggccc ggccccggtt tgcagcacat tcaccggctc
    16561 aaaggccgcg atgctgtccg gccaccgctg gatgtagttg cgcttgtatt tcggccattt
    16621 gacggagatg ccgggagcgg atggcggctc ctggaaatgg atggatgcgt cattgggctc
    16681 catccagtaa atcgtgaccc ggtccgcgtc caggctctcg cgctccgcga agtaatccgt
    16741 gacgccatcc gggcggatga agctgccata gggcagcttg cctcccacga tgaggttgaa
    16801 aagcatgacc gggtccagct ggtcatcgcc gttttccggc gcgccctggc gcgggcgcag
    16861 cttttctccc agatgggtgg tgatggtctc cacttccgcc acgcgccgga tgtccacgat
    16921 gtccgcgccg atgaactgat gcaccccggt gctgccctgg ttctcactgc cgaggtattc
    16981 gatgaaaagc cgcccctcaa tgttgtaggc atgcagtgat ggaggtccgt tttcgttgtc
    17041 gaaccagaag gtgcgatttt cggacgctga gccgatcgat cccggattgc tgcccacggg
    17101 cacatactcg tccgtcatgc cggtcacgaa gctgttgaag accgggttca cccgcacgat
    17161 gcgcccgggc gggatgttga cgatggggcc gtcaaagccc ttctccgtcc agtaaatgac
    17221 acgcgcggga gactgcgaag ccgaggacac agcgaaggtc tcctgacgga atttgtaggt
    17281 gggcgtgctt tcaccgggct tggtcgtgtc cggcacattg gaaacccagg tgatcgtcac
    17341 ccggcccgcc tggctggcga aaaccttgtt ggcgtgcggg ctgaagtaaa agggctggta
    17401 gggggcgaag gggcggctgg tgctggaact gatgttttcg ctggcattgc cggccagggt
    17461 gacggtcagc ccgttgatca tgttcacacg ctgccccagc aaggtggcgc cggccgtgag
    17521 ccccgcagtc acgctgctga tggtggccac gctgctgctg gtggagcttt gcgtcacggt
    17581 gacattgcct gtgggaacca aaggctgtgt ctgtcccgtc accgttcctt caatgagcgc
    17641 ggggtcgaag gattcccctg gctgcaccgg cttggacctc cagtaggtct gcgccaccgg
    17701 ctgtccatcc cagcgcaagt tcggacgcgt gatctcatcc cccatgaagt agcgtggcac
    17761 gccggaagca aagctgtggc cgaagctggc ccgcgccagc acgatggtca ccaccgggct
    17821 ggcgggaacc gtcacatcct gcgcgcgcgg gtagcgcccc gccgtgactc ccgccgtgga
    17881 gatggcgccc gccgtggcgg aggtcgtcgc gcgtccggca aactggggcg tcgttgtggc
    17941 gccgccgctt ggtgtcacgg gcggagcgat gaaaatggcg ggcggagtcg agtatccgct
    18001 gccgcccgtg ttcacggtga tcgaagtgac cacaccgccg gaaaccaccg ccgtggccgt
    18061 ggccgtggtg ccgctgggcg gcgcggcgat cgtgaccacc ggggccgggg agactgggta
    18121 gccgccgccg ccgccgcgca ccgtgatggc attgaccgcg cctccggaga ccgtggctga
    18181 tgcgcccgcg cggcgctggt ccagcagatt ggtggcgccg cctttgatct cgaactgcgc
    18241 atgggccgcg ccggcgagca ggagcaatag aaaggggaaa agaacacggt tcatgggaag
    18301 aggaatgacg aaatcagaat gaagaatgat gaagaccgta gggcgagcgt ggcgaaagcc
    18361 cgctcgtctc cgcagaggtg gagcaaacgg agatggtgca aatcagcgga agtgggtgtt
    18421 cgcagcctct ccgtgggggc gaaccgccaa gagccgggcg ggctgacgcc acacccggcc
    18481 tacggcgatt cgtgcgcggc accctggact gcggcagcct gctgccgctt ttccgaggca
    18541 gccctgctgc cgtcgaagtc tccacgcgtg cctttcgaga aaatcccccc gcactcaggc
    18601 cgaatgccga agcatgagag gaacatctct ctgccaccga cgccagcaag gctggctcca
    18661 gaaagcggca gctggctgcc gcagtccacg gggactgggc ctctccagat gccgggtgcg
    18721 ttttcataga cggaaaaagt gaagactcac tcggcatcat tggccgcctt cttcaggctc
    18781 acaggctgcg cggcgggttg gccattttcc agcagcagtt tctcgatggc ggccagcttg
    18841 gcctcgcgcg ccttgtccct ggcttcaaga agggccaatc gctcaagaag ttccgcgttg
    18901 gaaagacctg cggaagcgga atccgaattt gatgactcca gttttttcag gcgcgcctga
    18961 agctctttgt tggcttcctc cagcttgctg atctgggcgt tcttttcctg ccgcagttcg
    19021 cggaatgcct gaacagccag cgactcaaat ccgttgatcg tcagccgctt catcccatcc
    19081 atgcctttgg taacccagtc cggatacacc gcctcgacct cctgggcgat gaagccgttg
    19141 tacttgccct ccccgtgttt gaccgggtct atgtattcaa aggacacact cctgagcttg
    19201 agcagctttt ccagagaccc ctccacttcg acgatgtttt tcttcacccg ctgatcggac
    19261 agcgctgtcc atgaggtgcc gccgattttc tccgcagcac cgttgttcca gaaataatac
    19321 gcatccgcac ctgaaggatt gcgaaacatg gccaccaaat cgtctcccgg tatgcccttg
    19381 acctgaaaag tcacattatt ataaattcgg gttccgccaa cggtgacctc accatcagca
    19441 tgaatcgata aatcaacagc gctggaatcc ccctggaggt gcaggtcatt aaacccaatc
    19501 aatgcattga gacccgtaat gttgttgtcg ttggccacct ggatggctcc gttcacatgc
    19561 agtcttgccg ttggattcga ctcgcctatg ccgacattgc ccgcgctgtt gatggtcagg
    19621 cggggggtgt cattgtcatc aatgacaaag ccattgctgc cgttcatgcc gatgtcgtag
    19681 aactggccgt ctgacgtccg gttgaaccgc atctgcggtt cgttggcgcc aactccggca
    19741 acgaacaatg aaagagtcgt ggttccggac ggcggaaaaa acggcgtgct gaaggagccg
    19801 ttggtgccgg tcacgctcag gtttcctgtc gcgctgatgt tgccgttcac gtccagtttg
    19861 gagccggggt tggacttgcc tatgccgacg ttgttgcttg agttgatgca aatggcggag
    19921 ctcccggttc cggactgcaa gagcagcttt ccgttgaatg cgcgcacgat ggtgtccccc
    19981 tggaccgcgt ccgaggagta ggcgccgccc gcgccggcca tgcctaaagt ggcgcgctcg
    20041 gcgccgccgc tgttgtaaat cgaaaatcca acgctgtcac tggaggccga tccttgcagc
    20101 gcggtattgc cgaaaacatg cagcttgtgg ctggggctgg tcgtgccgat gccgacattc
    20161 ccgccgctcg ggttcagcag caggttctgc gccccaatgc cctccttgaa ggactggatg
    20221 taacccgtgc cggttcccgt gctgtccacg ccgatgcgca ggcgctcggt ggtgtcatcc
    20281 gccgtgacga tcatctgcgg actgccggtg ccggtgccgg cggcggtgac catgaaccgg
    20341 gcggttggat tcgtgttgcc cacccccaca ttgccgctgt ccaccgtggt caaagctgtg
    20401 ctggcgctgg tgccgagatt ttcggcgaat ttggcgcgga agctgaacgc ggaggagacg
    20461 atctgctggc gcggggtgat ctcattgtcc acagcggcgg tgccgtcatc cacggtgacg
    20521 ccaagatagc gcgtgctgcc cccgaacgca gtggcgatgt cggccagttt tttcgccgtt
    20581 tcgctgtagc caaactggct gcccgtggtg gccacaccct ggccgacaag cacgctgaac
    20641 tcgccgtcgg cgatggtgac ggtctgctgc tcggagtaga tgaggtttcc tgtgagcgtg
    20701 ttcgaagggt ggtcccagac gcggaagatg acggtgcggt tcaccggcgt gggcgagccg
    20761 acgaggccgc cggtgttgtt gagcactttg ccctggtagc tgatgaagcc cggcacaccg
    20821 gtggtgatgg tctgggcagc gaggagtcca ggcagcgcgc aaaacgcggc gaggatgagg
    20881 tggatgcggt gtgttttcat gttttctgaa tcggagcggt gagttgtgga accactaatg
    20941 gacactcatg ggcactaatg gtctgattgg aggggatgct gagagcttgg gcagtgaggg
    21001 cggcggttgg gttttcgaat ttcggatttc gatttttttc acggagtgac gctgatggcc
    21061 cccagctcgg agacacggcg cagctcaaag acgccggtga gatgcaggcc gtccccggtg
    21121 ccgacaccgg cgctgtcctt gtgcaggccg tggatgacct cggcataagt gccgccaatg
    21181 acggcgctgc cccagcctcc ggtggccgtg ctgccctccg gcggggcggt ggtgaaggtg
    21241 aaggtcacct cccgcgtgac ggcgtggctt tccttcccgg cgatgagcgc ggccccggtc
    21301 gggctcttgt tgtcatggtc agggtggtat tgatggacga agggattgac gggatcgtca
    21361 aaaggcaccg tgatggtccg cgtcagcgtg ccggggatgg ccacgctgcc tgtgcccgcc
    21421 gtcagcacgc ggtccagcgg catgtgggtg gccacgatgc gggctgcctt gtccttggcg
    21481 tcggccttga ggccggcctc cttggtgcag atgccgaagt catgcggcgc ggcggcgagc
    21541 tggccgagga agacctggga gagcacgcgc gcggtgccgt tgtccgccac atgcagcagg
    21601 tagcgcagcg ggaaggggcg gctggcgggc gtgatggcgt ccgcctgcgc cttgctttcc
    21661 accgccgtca cccgcgcctc gccgatccac aggccggcca gggaggtctt gcgcgcggtg
    21721 gcggggatga ggatgtcgaa aaggttggcc gcgtccgtga ggcgcagcat ggaggcgaag
    21781 agggcgtccg ccgtgccgcc catggccaca cggtcaatgc cgaaacttag ctccaccgcc
    21841 gattgcgggg cgatgacctc gttgtaagcc gtggcgatca gcgtctcctc ccaggctgcc
    21901 gtgccggtgt tgaaagtgcg ccgtgtcagc ggcaccggac cggtgatggc ctcctgcccc
    21961 gcaggcgcgg cattggaggc cacgggggcg atggtgagcg tcatcaccga ggcggtgcgg
    22021 ttcagcacgc gcgcggtgac gatggagccg ttgcggccaa aatccagccc gcccgtcagc
    22081 gagagcgtga catgcaccgg cgcataaaaa tttcccacca cctcggactc gaaccagtag
    22141 gcctggttgc ggtccagccg ctccgcggag ggtgagaaaa cctggatggg attggaggcg
    22201 ctgaggtctc cgccgacata cttgtaaatt ttcgagttcg tggcgatggc cgccgggaag
    22261 gtggcgaaat aggtggagaa gaaggggtag ttgccattca gcctggaggg aaagccgaga
    22321 aagttcgcgc cattgcgcac ccaggtgctg tttggcggcg cgggcttgtg aacaatgggc
    22381 acgttgtagg tgttcgaggc cgtgccggcg catttcacca ggtaggccgc cgggccggtg
    22441 aggtttggca gcgtgttggc gccgccgccg cgcacccaga cactccactc cggcgtgccc
    22501 tgcgtgggca gcagcggcgt ggtggtgaac tgcacctgcg tggggtttgg attccagcgc
    22561 cagacctcct gcacattggc cgtgtcaccg ctgttgggga aaagctgctc catcgtggca
    22621 tgggtggccg cgccgtgcag gtagatggag ttgtagccgc ctttcagcgc gtaggtggtg
    22681 ctctgccact gcgcctgggc cgtggcgcca atgatgaaaa acagaaagaa ccactgatgc
    22741 acactcatgg gcgctaatga cgaacgccga aggcgtgaaa cagccccctg tggcaagctt
    22801 aaacatgaac tcatcggctt gaaaaaatta gtgctcatta gtgtccatta gtggttcaaa
    22861 gttccgtcac ctcaccgtca gccggtaaaa ctcccgcgcg ccgcccggcg tcggaatcgt
    22921 gaaggcaggc aggatgccca cgtcctcggc aacgaagacc tgctggggcg tgccttccgc
    22981 cgtgaaggag aagggcaccg tctcccagcc ctgcatgttc aggctgcgct cgatggtgta
    23041 aaccttgccg gtgatggcga agaactccag ccgcacgaag ctggcggtct tttccttgat
    23101 ctgcatgtca atccgctcgg tggcgtcacc ggcgaaggtg cccgccacat actcgaacca
    23161 gttgctcata ccatcgccgt cgaagtcgcc gtcgcggtct atgagatgga tcggccacac
    23221 gccgttgcca tcagggtaat gcccggcttg atagagctgc cattgctccc agatgtccgg
    23281 cagcccgtcg ccatcgctgt cctcgcccag attgagatcc agccgcacgc gctcgccgcc
    23341 cttgcccgcc gtgagggtgc ccgccacctc gatggggtaa aaccgctccc cgttcatctc
    23401 caccgccagg ctgtaaggcg tgttggcctg gatggcggcg ctggtgtaga gcgcggtgcc
    23461 tggcctgcca gtgtctatgc gcaggctgat ctcgtagttt tgatccagtt gcagcccggt
    23521 gaccaccggc gcgcgcccca cctcggtctg tcctttgagc aaaatgatct gcgccccctc
    23581 cactgccagc gtctggccga cttgatcgcg aacgatgccg tagatcgtgt aatacggcgc
    23641 gggtggaaag gccatcaagc cgcccgttgc aatgcaggaa acacagaaaa aacggacggc
    23701 ggtattcatg acagcgagaa tggttcaaac cttatcttcg aaattttccc gccgatgtta
    23761 cccggaaagt tcaaaaaaag ctcttttttt atttcaccct ggtttgccgg ccttaaaatt
    23821 cgcaaaactc atcgtgccat ttcaccggaa aaaccccccg cctggatgaa aaaaaagaat
    23881 ccccccgcgc cggaagcctc cgcttttccc accacgcact ggacactcgt gcgcaccgtg
    23941 cagggaaaag acgccgccgc cgccgcgcgc gcgatggagg agctgtgcaa gggctactgg
    24001 taccccattt acgcctacct gcgacgcagc ggccacaacc ccgaggacgc ggaggacctc
    24061 acccagtcct tctttgaaaa gctcatcacc gaagacgcca tccagaccgc gcgcgaggag
    24121 gccgggaagc tccgctcctt cctgctcggc atcctcaggc acgcgctcag cgaccatctg
    24181 cgcaacaccg gcgcccagaa gcgcggcagt cactcacccc acgtctcctt tgacgagatg
    24241 aacgccgagc agcgctacgc cagcgagccg caggacatcc gcgaccccga gtggatcttc
    24301 acccgcgcct gggcgcacga gctgctggcc ggcgtgcggg aaaaaatgcg cgccgccttc
    24361 aagtccgccg ggcgcggcga aaccttcgac ctcctgctgc cctacctgct ctgggacgac
    24421 gagccgccct cgcacaggga gctggccgaa aaactcggct ccagcgaggc cgccacccgc
    24481 atcctcatcc accgcctgcg caccaagttc cgcgagctgc tgcgcacgga ggtcgcccgc
    24541 accgtgctca cgcccgagga gattccaggc gagctggcct ggctccaggg cgtgctggct
    24601 gaaaagtgac gcccggctta tttccctgta acacccgcgc cagttttccg aaaacagggt
    24661 tgcagcccct ttcattttcg tgacccccgc ctcctccagc tccaaacccg ccggcccctg
    24721 gcagcccccc tctctggagg aaatgcaggc catgctgccg caataccact tcctctccct
    24781 gctcggacgc ggcggcatgg gcgccgttta caaggcggtg cagacctcac tcgaccgcgt
    24841 ggtggccctc aaggtcctgc cgggcgccct ttccgccggc gatgacgccg gcgccgagtt
    24901 cgccgggcgc ttcaaaaacg aggcgcgcac catggccaga atgaggcacc ccggcatcgt
    24961 caatgtctgg gacttcggcc agaccggcac cggcatgttt tacatcgtca tggagtacat
    25021 ggacggcacc gacgccgccc agctcatccg ctcccagggc aggctgccgg aggcccacgc
    25081 cctctccatc accgcccacg tctgcgacgc cctcgcctat gcccacgccc agggcgtcat
    25141 ccaccgcgac atcaaaccgg ccaacatcct catcaaacaa accggcgagg tcaaagtggc
    25201 cgacttcggc ctcgccaagc gcagcgatcc cgcgctcagc ggcctcacca agaccaacat
    25261 ggccatgggc acccccgact tcctcgcccc ggaggccctc atccccggca tccccctcga
    25321 cgggcgcgcc gacctctacg ccgtcggcgt catgctctac caaatgctca ccggcgagct
    25381 gccacgcggc atgtgggaga tgcccggcgc ccgcctcggc accgacccgc gctttgacga
    25441 gatcatcacc cgcgccatgc aggccgaccg cgaagcccgc taccagagcg ccgccgaaat
    25501 ccgccgggcg ctcgacacca tcctcacccg gccaaagccc gcc
//
LOCUS       DAOMYJ010000054        25485 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Prosthecobacter sp. isolate AMR_MDS_5629
            NODE_699_length_25485_cov_20.421589, whole genome shotgun sequence.
ACCESSION   DAOMYJ010000054 DAOMYJ010000000
VERSION     DAOMYJ010000054.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867850
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prosthecobacter sp. (wastewater metagenome)
  ORGANISM  Prosthecobacter sp.
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Verrucomicrobiaceae; Prosthecobacter.
REFERENCE   1  (bases 1 to 25485)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 25485)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.30
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:34:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,742
            CDSs (total)                      :: 4,684
            Genes (coding)                    :: 4,676
            CDSs (with protein)               :: 4,676
            Genes (RNA)                       :: 58
            tRNAs                             :: 55
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25485
                     /organism="Prosthecobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_699_length_25485_cov_20.421589"
                     /isolate="AMR_MDS_5629"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1965333"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..856
                     /locus_tag="PLP58_07765"
     CDS             <1..856
                     /locus_tag="PLP58_07765"
                     /inference="COORDINATES: protein motif:HMM:NF013530.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HRJ08447.1"
                     /translation="PGREVLAVSRFSSARARAGLEAHGVKVIPCDLLDRAAVNALPDA
                     PNIVFMAGHKFGTSDSPELTWMMNAVLPAIVAERFVKARIVVFSTGCVYPFTPVDSGG
                     SREEDALAPPGEYAHSCIARERVFTHYSKQQGTRCLIYRLNYAIDLRYGVLHDVARKV
                     WLGEPVDVTMGHANVIWQGDANARALQCLARAQSPPAILNVTGRETVRIRELAERFGA
                     LLGRAPVITGQEAPTAWLSNAAKSYDWFGETSVSLDEMIEATAAWVRQGGGSLGKPTH
                     FETRDGNF"
     gene            843..1862
                     /locus_tag="PLP58_07770"
     CDS             843..1862
                     /locus_tag="PLP58_07770"
                     /inference="COORDINATES: protein motif:HMM:NF012904.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HRJ08448.1"
                     /translation="METSELRAALQSGLCIPAHPLALNAARKLDERRQRALSRYYTAA
                     GAGGLAVGVHTTQFAIREPRHGLFEPVLALAAEEMRRASRPLVRIGGICGPTAQAVRE
                     AELLRAHGYHAGLLSLAALRDADEDALITHARAVAGVIPIVGFYLQPAVGGRVLPLSF
                     WRRFAAIENVVAIKIAPFNRYQTLDVIRALAESGREDIALYTGNDDNIVADLITPFSF
                     GGKELRITGGLLGHWSVWTQKAVELLRRCKEDAATPGLLRLGVEITDSNAAFFDAAHG
                     FHGCIAGLHEMLRRQGLLEGIWCLDENEGLSPGQAEEITRVHRAYPHLHDDAFVARHL
                     DEWLR"
     gene            1907..2257
                     /locus_tag="PLP58_07775"
     CDS             1907..2257
                     /locus_tag="PLP58_07775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009959288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HRJ08449.1"
                     /translation="MTSEPRFAVAQLDDIPPTPCPCGQARRAFKEPWNNLATVHLTEI
                     SADSRAHYHKKMTEIYIVIEGEGHLEADGRIIPLKPMTTVMIRPGCVHRAVGNLRLII
                     VPSPPFDPEDEFEV"
     gene            complement(2304..2378)
                     /locus_tag="PLP58_07780"
     tRNA            complement(2304..2378)
                     /locus_tag="PLP58_07780"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2344..2346),aa:Thr,seq:tgt)
     gene            complement(2447..3781)
                     /locus_tag="PLP58_07785"
     CDS             complement(2447..3781)
                     /locus_tag="PLP58_07785"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HRJ08450.1"
                     /translation="MRHILIPACLFACLLQAAPKPNIVFIMADDLGWADVAFHGGNAP
                     TPHLDKLAREGVEMTRHYVAPVCSPTRTGLMTGRFWSRFGVTSPQNERALPWDTITLP
                     SALKAAGYETALTGKWHLGSLPEWGPNHYGFDHSYGSLAGGVGPYNHFYKQGPYSVTW
                     HRNGKLITEDGHVTDLITGEAVKWISGRGDKPFFLYVPYTAVHLPVKEPRSWLDRVPA
                     AIMDEVERHYAACVMHLDDSVGRIITALEQAGKRQNTLLIFTSDNGGSTSQNNDTKYP
                     ADDYPKGALPGSNLPLRGKKGDLHEGGIRVPTVASWPGVLKPGRFEDPAHISDWMPTF
                     CALAGHAPERDLKWDGVNIWPALSGDGALAARALYWTAPGFRSRAVLEGGWKLVTTKG
                     KPGEPEQAELFDLNTDPGEARDLASSQPERAAALRATLEKLAASDNDSKVPR"
     gene            3894..4292
                     /locus_tag="PLP58_07790"
     CDS             3894..4292
                     /locus_tag="PLP58_07790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ08451.1"
                     /translation="MARRAQSGLKPGHLLGLLALLVAGIGGGYALLNRGSDPMTGLTP
                     LSAEEYMESSTALGGNVYKVEGTVDDRLDNWRAADGRLFSVQVSESSNFVPVWVPPAL
                     EVNIQRGQRYIFKVRVLETGVLEVLQLIKA"
     gene            4300..6228
                     /locus_tag="PLP58_07795"
     CDS             4300..6228
                     /locus_tag="PLP58_07795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ08452.1"
                     /translation="MKTAALLACCILPGMLTAQVLPAPSLTPAAQPAPAQSSQPSSSG
                     GGQSGGQSGGQQGGMFGGLVPHVDSGSETVTWDGKMWNATNNRFARAKFEIYLATPEA
                     DSADDQAYRDLIAQIIQALAPTRQGGADVSKAFAMLPEAGSFAIDGGICNTIANGVYD
                     VWLAQRKVNNLRSVNDAMLKRRKDLEWNTEQGLNSAPLSPSTTGGSTKGGAGGTNRQQ
                     TVQQQQTNSMTMGRVSGYIKSIAEIEARMKLNETAIGLSEVTSKTQFQALMVQLFMQR
                     RFEHAIIACRLYPHMFRDGDSVLQLDDNSDLKKMFSAGMGVPPTVSLIDGFAAEAIRG
                     VDEAVVAFEQLVLRQDFDSASKRLMEAYAIGEYLPRIRRLPMATKLKVLDYVRDGNQL
                     INALEMRDFGLAEELVTKLRGMSRDFDFSKPQAAIETARALCDMRITAARAAILKGDQ
                     ITYATELKGAAEIWPTNPKLRELSNMVGQNSDIQAQALLDLDRLLSQRNFRQIFNDQG
                     RFLAAAINDPQRQEQIKKVLVDMNRVNMVVHGASQMAASNAFGAWEQVEKEYHNFPED
                     PEITRLRSELSVKASEYVSAIEKARQHEQRRQTGSAIAWYLKARRLYPPSEFARDGIQ
                     NLVGSLNSADSAPGGAQP"
     gene            6225..7076
                     /locus_tag="PLP58_07800"
     CDS             6225..7076
                     /locus_tag="PLP58_07800"
                     /inference="COORDINATES: protein motif:HMM:NF013429.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HRJ08453.1"
                     /translation="MNRRRLLRQSALALGASVLPGAPAQGGQFTGKIRKSLKWSMVAE
                     AGNLPLAECFKKLRECGFEGVEPSLSHVPDDRVDDWLAASKASGLIIDGTVGGRAATL
                     ESGVDLTRKLGGDSMLVVMSYDQKAPLKPQWNAARDRLKEAAPHAQNQGVKILVENVW
                     ATFLISPFDMARFIDEVGSPWVAVHLDIGNQMRWGVAEHWVEVLGERSQKLDVKEYDL
                     NKAMSEGMRKGFDTPIGEGSINWPAVRAELAKIQFTGWAAAEVKGGGWDYLADVARRM
                     DRVLDLA"
     gene            complement(7089..8624)
                     /locus_tag="PLP58_07805"
     CDS             complement(7089..8624)
                     /locus_tag="PLP58_07805"
                     /inference="COORDINATES: protein motif:HMM:NF024753.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-like beta-propeller repeat protein"
                     /protein_id="HRJ08454.1"
                     /translation="MVLSTLPNLHADWPQWRGPGRGGVSTDTTPIAEVFPEAGLTQVW
                     ESGFIPSDHYGGHGSPVVAGERVFLSVVWHDRVVSETREIDSEVMQQLNYRGTSPELR
                     EKMEAARLSMSSRLRGEKLDEWAKQWVKDNMTEKEQVSLTNWAVSRFRAGKTAFPLEE
                     LDKVSKRQGKPFASVAEFKQWLEDEKFSDALKEKLLSAVPNTIKVAKDVLVCLDLNTG
                     KELWKYEAEGKPTGRSSSSTAAVMDGQVFFAGSTHLYAVGAEDGKLRWKAELPGKGPA
                     ASPMVEGGLIYMAAGHAQAFAAKDGSLLWEQKAAKGNTGSPAWWKPASGPPVVIINGS
                     GSLYGLAPDTGEVKWTVEGGGQSTPVTEGDWLVVYSGSKDVGLRAYQYQKDGAPKPAW
                     SHFWVTVRYSGSPIIHDGHVYLTCGGKHQCVELATGKITWLENEMNSTITSPILADGK
                     ILVYENNGSHLRVMRARSDQYETLARVKLGGMGCTSPALSNGRLIVRQKEKLACFDLR
                     PQQ"
     gene            8903..12622
                     /locus_tag="PLP58_07810"
     CDS             8903..12622
                     /locus_tag="PLP58_07810"
                     /inference="COORDINATES: protein motif:HMM:TIGR02601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter-associated beta strand
                     repeat-containing protein"
                     /protein_id="HRJ08455.1"
                     /translation="MKTPSLPIPHGLRATLVIIVSAIVLPAISNARLYWDTNGSTPGS
                     GNAAPSGVWNLSAQNWNNDQGDGTPLIWLNDGTGQAVFSAGSDATGAYTVNLSAGEII
                     RLAALSLRSGSLTLTADAAADVLDFGTMTAALVVEAGSVLDVQTQVLGTAGLIKSGTG
                     VLRLADAGPSGAWNVSNGRLILGANQSVTTLTLGGSSTSVAALELEDGIVLDLGGNIT
                     LTSLSQQETVAVQGGVLRLNGSRTITIPNVPAPVDIEISSVLADGSEVSSLTKSNNAG
                     TLLLSGANTYTGATVLAGSNSGTLLVQGNRASIAGSFSIVVNGSTLTLGTALDTDAVN
                     RLRDDAAITLNGQTGAGASLNYNGSDFATQSIHQETIGAITVGGRHRSFITLTPGAGD
                     EVVIQAASMSRAENGILLLRGSNLGAASGTADSSRLQLADTSSLVGGDNSTTGRAILP
                     WAVGDTSVAGPGTGFLTYDEQTGLRLLDNATEFVAPASAGEGLNVRKSATGNLTLTSS
                     VAHNTWTNAATGTVALGAGVTLGLESGAVLFTANGTFAGGTFALAETRQGIIHLAANA
                     AVTATINSSLSGGNGLLISGTGTGNKIVVLGGDNSFTGGVLVYAGILQLNHPGALNSS
                     GVNSLVLQAGGTLRLNGNSITLSGLDGAGTIVNNNAANAAELTINGGGTHTGALNNGS
                     AAALGLVKTGNALLLLGGNNNYTGSTVIQQGSVHVQPAGNNPGGNGGLRGTSKITIGK
                     SALLQINNGNVAGSNNADRVNDSAPLLLLGSLTYNHSASNLAYEETLGALILGQSAAQ
                     ITASIAGASGTSVLTFTSLAGREHGGVLNFTGTSLGAADSNAGRNQIFFNSGVAYGFI
                     GGWATIGNEFAKYTAVTGVTGFLADDYFTGVQEDWTPATHAKPDAAQILDSDRNVLSL
                     NLTGGGPKNVNLGGNVLNIGSGGLIKQGGSAGTTGAANVATISQGSITAGGAAAAELF
                     IRVTGANLNISAAITDNLGDGDTPGAVTVVKSGPGTAVLTGNNIHSGGFFLHEGVVVV
                     NNNGRTGTGSVHVGPGARLGGNGIIGSLSPGAGIEIEPGGTLFVGQSTGLGAQTLTLR
                     AENGFLISGSVELDIVGGAAGGLLNPQTGNNDRLIFTTATGVGFGTPDLSGALLKVNT
                     NITLTPDAWTVGSAWKLFDWYGLTSGFDNLPDTGTRHGNPNHLPDLSSLGLAWDWSQL
                     YTDGSLSIVVPEPGRALLLTLGMAFSLLRRTRPMR"
     gene            12638..13609
                     /locus_tag="PLP58_07815"
     CDS             12638..13609
                     /locus_tag="PLP58_07815"
                     /inference="COORDINATES: protein motif:HMM:NF013741.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CNNM domain-containing protein"
                     /protein_id="HRJ08456.1"
                     /translation="MTALLLLLTATVLGISFLCSLTEAVLLSLNPLHLKLQERRGMKM
                     AARWLALKNRIERPISAILVFNTIANTGLASLSGAIFAGLYGSEWLWAYSVLMTLAVL
                     FGAEMTPKILGVHHAERLAPKLIRPLTFALFLCHPLVVVMEEFCKRLKTSAGDKKSPS
                     DHIMDIITLVQAARAEQLLHNREELIIIHAATLSARRVKTAMVPRDEVKVFDERLTLK
                     ENVLAAGAKLHRSYPVSADGTLERVTGYIRVRELFVQNLMETGGSDWRKLIRPSLYID
                     AKASLTQLLALYLDRQEVAALVDTPEGRITGWITMDDVMKVLMGARI"
     gene            13675..14226
                     /locus_tag="PLP58_07820"
     CDS             13675..14226
                     /locus_tag="PLP58_07820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009959463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HRJ08457.1"
                     /translation="MKLTKRGEYALRTLIRLGVAAWNNEGVISVSVLAEKERLPFKFL
                     EAILFDLRNAGYVESVRGKYGGARLAKPLNAVKMGEVVRLIDGKLAPIGCASETEYEK
                     CSCPDEEHCGLRMLMIDVRNAVANILDKYTLEDVVEVTLRKQLITASTAKKSTRKAGH
                     ERHADPADGFLAVLLNTAPARRR"
     gene            14322..14507
                     /locus_tag="PLP58_07825"
     CDS             14322..14507
                     /locus_tag="PLP58_07825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009959462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YezD family protein"
                     /protein_id="HRJ08458.1"
                     /translation="MQQAPHTQDWIEIVRQKVAAMRFGSVQIIVHEGRVTQVESTEKT
                     RLPGDIPPASSNRRASA"
     gene            14545..15507
                     /gene="cysK"
                     /locus_tag="PLP58_07830"
     CDS             14545..15507
                     /gene="cysK"
                     /locus_tag="PLP58_07830"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009959458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004124 - cysteine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HRJ08459.1"
                     /translation="MAIQTSILETVGRTPLIKLNRIASGLPVTIALKGEFANPLGSVK
                     DRIGSAMIESAEKSGILKPGGEIIEPTSGNTGIALAFVAAARGYKLTLTMPESMSLER
                     RTLLALLGANLVLTPAKEGMKGAIRKAEELLAATPGAWMPQQFENPSNPEVHRRTTAE
                     EIWEDTHGAVDIFIAAVGTGGTITGVSEVIKSRKPTLVSIAVEPQDSPVITQTRNGEP
                     LVPGPHKIQGTGAGFVPRNLNLGIVDDVIQVSNEDAIHTARRLALEEGILAGISTGAN
                     VWAALQVAARPENQGKLIVTVGCSTGERYLSSPLAEEARNRVQS"
     gene            15521..16288
                     /locus_tag="PLP58_07835"
     CDS             15521..16288
                     /locus_tag="PLP58_07835"
                     /inference="COORDINATES: protein motif:HMM:NF016817.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinylglutamate desuccinylase/aspartoacylase
                     family protein"
                     /protein_id="HRJ08460.1"
                     /translation="MKLLPPLNVRRSHAPRAAHSNLLTLENHILDEGFFIPPKIISRP
                     EKPGQGYKFGVFAGIHGDEEAGTLAVQELLKWSGEQPDETQDFELHFYPVCNPSGRNL
                     GTRHNHRGLDLNREFWSGTIEPEVLYLEGELRRERYDGIISLHSDDDAVGCYGFVSGD
                     ILSEHLLDPALAAAAAHIPREVAPVIDGFLAERGIIKEGYLGILSAPPEQRPRPLEVV
                     FETPGLAPMQVQVAATVAAVKAMLHGYRQLHAYAANL"
     gene            16319..17299
                     /locus_tag="PLP58_07840"
     CDS             16319..17299
                     /locus_tag="PLP58_07840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009958613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HRJ08461.1"
                     /translation="MPTRRHFLLSAAALTGCRPAADPEGVLRIGHFPNVTHVQALVAR
                     SMSREGRGWFEQRLGLEVRWFTYNAGPSVAEAVFARSLDAAYMGPSPLLNAYVRSDGR
                     EMRVLSGAVNGGAALLTHPQSGIEKAADFRGKKLATPQLGNTQDVQARAWLRAQGFQV
                     SLTRHDVYVIPTKNADQLALFAKGDLDAIWTTEPWATRLEVESDARVFLEDTTTPATL
                     LAARADFAGQKPDLAEKLATAHRELTAWIRQNPAETAERVRAELEALTTARISPELLA
                     RSMRRLVLTDEVSPAQLQTLLTQARDAGFLRGAPALDALLSTCATLRTDS"
     gene            17296..18102
                     /locus_tag="PLP58_07845"
     CDS             17296..18102
                     /locus_tag="PLP58_07845"
                     /EC_number="7.6.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012419461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRJ08462.1"
                     /translation="MTASATLLPEAPPAKLRIESVSKTFHGTRGDVLALDTTTLTVDE
                     GEFVCLVGPSGCGKSTLLNLVAGLDTPSAGRLLMDGKAITSPGSERMMMFQEHALFPW
                     LDVIGNVIFGLKLKDGLNAKERVEVAKYYLKLVGLERVMKSSIHELSGGMRQRVALAR
                     SLAPNPRVLLMDEPFAALDAMTREQLYADLQKISAAQKKTVIFVTHNVREAVCLGDRV
                     ILFSPNPGRIREQFKVQLPRPRDINDPALSVLAGEITRALKSHTSAPADE"
     gene            18099..18860
                     /locus_tag="PLP58_07850"
     CDS             18099..18860
                     /locus_tag="PLP58_07850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009958615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRJ08463.1"
                     /translation="MKRLLIATAFFALLILAWHLLVRTGVWSPVLLPGPAAVGEYLWA
                     ALKDGSLASSSWVTLQRLLLGYLIGCAIGLPLGILTARSKTLNDTLGVLALGLQTLPS
                     VCWVPLALIWFGQSESAMLFVVIMGTLWSVLISVDNGVRNLPPIYTRAARTMGSSGFH
                     TLRHVILPASMPYVISGMKQGWAFAWRSLMAAEIYVTLTGYGLGHLLHFGRELNAMDQ
                     VAGVMLVIVLIGLLADKILFSPWERFLHRRWGLNG"
     gene            19082..21421
                     /locus_tag="PLP58_07855"
     CDS             19082..21421
                     /locus_tag="PLP58_07855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009965375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ08464.1"
                     /translation="MKPILLLSVYFVCSVVNSLAQEPRSISGIYPSLAMFNNEGECGT
                     GAVVPWADRLWVITYGPHLPFGSSDKLYEITPELKQIVRPESVGGTPANRMIHDESQQ
                     LFIGPYVIDAERKVRVIPPSIMPGRLTGNARHLTDPAGKIYYATMEEGLYEVDVKSLA
                     VKNFIRDGNPIKKDFKVETQLSNLESQLPGYHGKGLYSSQGRVIYANNGDRDKRVLTD
                     PTTPSGALGEWTGSGDWQLVRRNQFTEVTGPGGIHGSDANAPVWSIGWDAKSLILMLL
                     DGGKWQSFRLPKVSHSYDGAHGWNTEWPRIRDIGEENLLMTMHGAFWSFPKTFSASNT
                     NGIRARSAYLKVIGDFTRWNDRLVFGCDDSAKSEFLNKRQVKGGIDSIGQSQSNLWFT
                     SADTPGKLGPAHASGAVWLHESVKAGNVSDPFLVAGWKHRSVWHIDHATGESKRVEIS
                     SDDEWLRVKAASDSKDFSVLITLSNGDSRSTEPDAIFDGIARIGDENAQTGLFRPRGE
                     NKRTLSFATKDGYYEMGADLALKRVEDAAAKAFLERAVALPKNVITLDEASVLVVDDR
                     GRRWRLPRASKAYDAATKAGELRICREVATERDMLSACGTFFELPAENADGFAKIRPI
                     CSHDLRVTDFGGYRGLFLMSGIKPDAKAGDHILRSDDGKAALWAGAIDDLWKLGKPRG
                     EGGPWKATKVKAGQKSDPYLMWAYDQRTLTLSHDQNEPVSYHIELDLTGTGLWVTWQW
                     REVSPGEDALLVFDPALQARWLRVTASEDCTATAQLRHE"
     gene            21508..25467
                     /locus_tag="PLP58_07860"
     CDS             21508..25467
                     /locus_tag="PLP58_07860"
                     /inference="COORDINATES: protein motif:HMM:TIGR02604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HRJ08465.1"
                     /translation="MMMRRYFIPLLSCVLVQAAAAEVVARKWSGTLNVPDPVACTVDE
                     YGRVYVAATTRRKAADLDIREHPMWIADDVGLTTVEEKREFLKRELAPGRLRLPRGGL
                     KDHNKDGSIDWQDLTVHSERVYQLRDTNGDGLADKMTVFAEGFNTEVTGIAAGILYHD
                     GWVYLTCAPDLWRLKDTDDDGVADVREVVAHGFGMHIAYAGHDMHGPRLGPDGRIYWS
                     IGDKGVSVTSKEGKKFHLPHEGAVLRVEPDGAGFEVFARGLRNVQEIAFDDFGNMFGV
                     DNDADLPGERERLVYITDQSDSGWRCGHQYMKGASRWILEDVWLPQQPWKGGGSKQPL
                     FITPPLANYSDGPAGFLHEPGTALGASLRGHFLLNQFPSGRMDAFKLEPDGAAFKMTD
                     PRTIHNGVMGIGMAWGVEGALYFADWDGGYPLDEKGGIWTLDSAADKDAAARRETMEI
                     IATGFGSRPDSDLQKLLGHADRRVRMSAQLELAKRGQWDALLAIAADTGAPLLARVHA
                     IWGAGIGLRHGKVPETLLHERLAAETDAEIQTQLVKIIGDAKPSVAATRAVDRMMTSE
                     SPRVRLHAALALGRMAPVSAGLSLAKVELNNAAFYLLDRVERDAHDPFLRHALVSGLA
                     GCMSAEDLASRFDSESEPVRLVCALALARQRSPLIKDYLKDSSDAVFDEVCRAIHDDG
                     GIPDVLPELAALLDGGRPLTPPARRRAINANLRMGQPVNAARLLQHALNQPPEQAVEA
                     LRTLLVFSKPPRLDLVDGMAGTYPRMDENALSPSLIETWKSDLLALKDPSLKTAVVEL
                     LIQLQLEVDASVLLAIAVDEKAETTLRIGALRLMTAKNPQAPAVAQALEKTSGKTAPE
                     ELRMESLALTARHQPERALGEISRVLDSGTLREKQAAISLLAAHPGSGADALMTAWLQ
                     KLADEKVETGLRLDVIEAAQGRESVAARVTAWQQTRKTPPAEDLIEGGDIKAGREIVN
                     NHIGANCVACHTVEAKEGSQVGPPLKGIGTLRARAELLESLVNPVAQIAEGYGLVSLT
                     LKDGGSHAGSLVSESDKAVRLRLPDGALKEIPREIILAQTPPVSVMPPMLGILTPREV
                     RDVVAYLASLTGAPDKKPQPAKTAPKAVVKTAPAPAKKAPASTPAKSTPGAPKAAVKA
                     APEIAPPSVPQPAPAAPAPASSMPVELADIPRAIPVDDNGQVMTEPPKQEPRKGGITL
                     RFGNRKKEEAPPPPLVMPEPMSADDAGETKAEDKGENKPPAPAPAKEAAKETAKAGAK
                     SEPAAPAPKAEAPAKADAKDAAAKSDAPPPAEMPAEAKTEEKKGFFSRFRSKKNEEE"
BASE COUNT         4773 a         8776 c         7639 g         4297 t
ORIGIN      
        1 gccggggcgg gaggtgctgg ccgtctcgcg cttttcctcc gccagggcgc gcgccgggct
       61 ggaggcgcac ggcgtcaaag tcatcccgtg tgatttgctg gaccgcgccg ctgtgaacgc
      121 cctgccggac gcgccgaaca tcgtcttcat ggccgggcat aaattcggca ccagtgactc
      181 gcccgagctg acctggatga tgaacgcggt gctgcccgcc attgtggcgg agcgcttcgt
      241 caaagcgcgc attgtcgttt tctccaccgg ctgcgtgtat cctttcacgc cagtggacag
      301 cggcggctcg cgcgaggagg acgcgctggc gccgcccggt gaatacgcgc actcctgcat
      361 cgcccgcgag cgcgtcttca cccattattc caaacagcaa ggcacgcgct gcctgatcta
      421 ccggctgaac tacgccatag acctgcgcta cggcgtgctg catgacgtgg cgcgcaaggt
      481 ctggctcggc gagccggtgg atgtgaccat gggccacgcg aatgtcatct ggcagggcga
      541 tgcgaacgcg cgcgccctgc aatgcctggc ccgtgcgcaa agcccgcccg cgattttgaa
      601 tgtcaccggg cgcgagacgg tgcgcatccg tgagctggcg gagcgttttg gcgcgctgct
      661 cgggcgcgcg cctgtcatca ccgggcagga ggcgcccacg gcctggcttt cgaatgccgc
      721 gaagtcttat gattggttcg gcgagacctc ggtgtctttg gatgaaatga tcgaggccac
      781 cgctgcctgg gtgcggcagg gcggcgggtc actcggcaaa cccacccatt tcgagacacg
      841 cgatggaaac ttctgaactc cgagctgctt tgcaaagcgg cctctgcatc cccgcgcatc
      901 cgctcgccct gaacgccgcg cgcaaactcg acgagcgccg ccagcgcgcg ctcagccgct
      961 actacaccgc cgccggcgcg ggcggcctgg ctgtgggcgt ccacaccacg cagttcgcca
     1021 tccgcgagcc gcgccacgga ctctttgaac ccgtgctcgc cctcgccgcc gaggagatgc
     1081 gccgcgcctc tcggccgctc gtgcgcatcg gcggcatctg cggccccacc gcgcaagccg
     1141 tgcgcgaggc ggaactgctg cgcgctcatg gctaccacgc cggattgctc agcctggccg
     1201 cgctgcggga cgcggatgag gacgcgctca tcacgcacgc gcgggccgtg gcgggtgtca
     1261 ttcccatcgt cggcttttac ctgcaacccg ccgtcggtgg ccgtgtgctg ccgctttcgt
     1321 tttggcggcg ctttgcggcc atcgaaaacg tcgtcgccat caagatcgcg cccttcaacc
     1381 gctaccagac gctggatgtc atccgcgcgc tggcggagag cgggcgggag gacatcgccc
     1441 tctacacggg caacgatgac aacatcgtgg ccgatctcat cacgccattc agcttcggcg
     1501 gcaaagagct gcggatcacg ggcggactgc tcggccactg gtccgtgtgg acgcaaaaag
     1561 ccgtggaact gctgcgccgc tgcaaggagg atgccgccac gcccgggctg ctgcgcctcg
     1621 gcgtcgagat cacggacagc aacgccgcct tcttcgatgc cgcgcatggc tttcacggct
     1681 gcatcgccgg gctgcacgag atgctgcgcc gccagggact tctggagggc atctggtgcc
     1741 tcgatgaaaa cgagggcctc agccccggcc aggccgagga aatcacccgc gtccaccgcg
     1801 cctatccgca cctgcatgac gatgcttttg ttgcccgtca cctcgatgag tggttgaggt
     1861 agattttcct caatccgcat tccacaatcc gaaatccgca atccccatga cctccgaacc
     1921 ccgcttcgct gtcgcccagc ttgatgacat tccgcccacg ccctgcccat gcggccaggc
     1981 gcggcgcgcc ttcaaagagc cctggaacaa ccttgccacc gtccacctca ccgaaatcag
     2041 cgccgacagc cgcgcgcact accacaagaa gatgacggag atctacatcg tcatcgaagg
     2101 cgagggccac ctggaggccg acggccggat cattccgctg aagcccatga ccaccgtcat
     2161 gatccgcccc ggctgcgtcc accgcgctgt gggcaatctg cgcctcatca tcgtgccttc
     2221 gccgcccttc gacccggagg atgagtttga ggtgtgagcg ccctgcgttg gtgtcaaggc
     2281 ttcagccggt ccagcgtttc gtctggagcc gagtgtcgga tttgaaccga cgacctgatg
     2341 attacaaatc aactgctcta ccactgagct aacccggcgt tgagggggaa cgagcgtccg
     2401 ctataattcc actctccgcc gcgggcaagg tgaatctggc ccggtgtcag cgcgggactt
     2461 tggagtcgtt gtcgctcgcg gccagttttt ccagcgtggc gcgcagggcg gcggctctct
     2521 ccggctggct ggacgcgaga tcacgcgcct cgccggggtc ggtgttcaga tcgaacaact
     2581 ccgcctgttc cggctcgccg ggcttgcctt tggtggtgac gagcttccat ccgccctcca
     2641 gcacggcgcg ggagcggaag cccggcgcgg tccagtagag agcgcgcgcg gccagcgcgc
     2701 catcgccgct gagcgcgggc cagatgttga ctccgtccca cttcaaatcc cgctccggcg
     2761 catgacccgc cagagcgcag aaggtgggca tccagtcgct gatgtgcgcc ggatcttcga
     2821 agcggccggg tttgagcact ccaggccagc tcgccacggt gggcacgcgg atgccgccct
     2881 catggagatc gccctttttg ccgcgcaggg gcaggttgct gccggggagc gcgcctttcg
     2941 gatagtcgtc ggcggggtat ttggtgtcat tgttttgcga ggtgctgccg ccgttgtcac
     3001 tggtgaaaat gagcagcgtg ttttgtcttt tgcccgcttg ttccagcgcg gtgatgatgc
     3061 ggcccaccga gtcgtccaga tgcatgacgc aggccgcgta atgccgctcc acctcgtcca
     3121 tgatggcggc gggcaccctg tccagccagg agcggggttc cttcaccggc agatgcaccg
     3181 cagtgtaggg cacataaaga aaaaagggct tgtcgccgcg cccgctgatc cacttcaccg
     3241 cctcgccggt gatgagatct gtcacatgcc catcctcggt gatcagcttc ccgttgcggt
     3301 gccacgtcac gctgtatggg ccctgcttgt agaagtggtt gtaaggtccc accccgccgg
     3361 ccagggagcc gtaactgtgg tcaaagccgt aatggttcgg tccccattcc ggcagcgagc
     3421 cgagatgcca cttgccggtc agcgccgtct cgtagcccgc cgctttcagc gcactgggca
     3481 gggtgatggt gtcccagggc agggcacgct cgttttgcgg agaggtgaca ccgaagcggc
     3541 tccaaaatct gcctgtcatc aatcccgtgc gcgtggggct gcacacgggc gccacataat
     3601 ggcgggtcat ttccacgcct tcccgggcga gcttgtcgag gtgcggcgtc ggtgcgttgc
     3661 cgccgtggaa ggccacgtcc gcccagccga gatcgtcggc catgatgaaa acgatgttcg
     3721 gttttggcgc ggcctggagc aggcaggcga acaggcaggc gggaatgaga atgtggcgca
     3781 tgacaaatcc gcaacgcgcc gcagtcacgg cacttgcgcg gcattttcaa gtttccgggt
     3841 cttgccgcag ggaggttgag cctttaagct cgcgcggaaa tcattcaatt ctcatggccc
     3901 gccgcgcaca atctggactc aaacccggcc acctcctcgg actgctggcc ctgctcgtgg
     3961 ccggcatcgg cggaggctac gccctgctga accggggcag cgacccgatg accggtctca
     4021 cgccgctgag cgcggaggag tacatggaaa gctccaccgc ccttggcggg aatgtttaca
     4081 aagtcgaggg caccgtggac gaccgcctcg acaactggcg cgccgcggac gggcggctgt
     4141 tcagcgtgca ggtcagcgag agttccaatt tcgttcccgt ctgggtgccg cccgccctgg
     4201 aggtgaacat ccagcgcggc cagcgttaca ttttcaaagt ccgcgtgctg gagaccggcg
     4261 tgctcgaagt cctgcaactc atcaaagcct gacccatcca tgaaaaccgc cgccctcctc
     4321 gcctgctgca ttctgccggg aatgctcacc gcccaggtgc tgcccgcccc ctcgctcaca
     4381 cccgccgcgc aacccgcgcc ggcccagtca agccagccgt cttcctccgg cggtggacag
     4441 tcgggcggcc agtccggcgg acagcagggc ggcatgttcg gcggcctcgt gccgcatgtg
     4501 gactccggca gcgagacggt gacgtgggac ggcaaaatgt ggaatgccac caacaaccgc
     4561 ttcgcccgcg ccaagttcga gatttacctc gccacaccgg aggccgacag cgccgacgac
     4621 caggcctatc gcgatctcat cgcccagatc atccaggccc tcgcgcccac gcgccagggc
     4681 ggcgcggatg tttccaaagc cttcgccatg ctgccggagg cgggctcctt cgccatagac
     4741 ggcggcattt gcaacaccat cgccaacggc gtgtatgacg tgtggctggc gcagcgcaaa
     4801 gtgaacaacc tgcgctccgt gaacgacgcc atgctcaagc gcaggaagga cctggagtgg
     4861 aacaccgagc agggcctcaa cagcgccccg ctttccccca gcaccaccgg cggctccacc
     4921 aagggcggcg cgggcggcac caaccgccag cagaccgtgc agcagcagca gaccaactcg
     4981 atgaccatgg gccgagtctc cggctacatc aaaagcatcg ccgaaatcga ggcgcgcatg
     5041 aagctcaacg agaccgccat cggcctctcc gaggtcacca gcaagaccca gttccaggcc
     5101 ctcatggtcc agctcttcat gcagcgccgc ttcgagcacg ccatcatcgc ctgccgcctt
     5161 tatccccaca tgttccgcga cggcgacagc gtgctccagc tcgacgacaa ctccgacctg
     5221 aaaaaaatgt tctccgccgg catgggcgtg ccgcccaccg tgagcctcat tgacggcttc
     5281 gccgccgagg ccatccgcgg cgtggacgag gccgtcgtcg cctttgagca gctcgtcctg
     5341 cgccaggact tcgacagcgc cagcaagcgc cttatggagg cctacgccat cggcgaatac
     5401 ctgccgcgca tccgccgcct gcccatggcc accaagctca aagtgctcga ctatgtgcgc
     5461 gacgggaacc agctcatcaa cgccctcgaa atgcgcgact tcggcctcgc cgaggagctc
     5521 gtcaccaagc tgcgcggcat gtccagggac ttcgacttct ccaagcctca ggctgccatc
     5581 gagaccgcgc gcgccctttg cgacatgcgc atcaccgccg ccagggccgc cattttgaag
     5641 ggcgaccaga tcacctacgc caccgagttg aaaggcgcgg ccgagatctg gcccaccaat
     5701 cccaaactgc gcgagctttc caacatggtc ggccagaaca gcgacatcca ggcccaggcc
     5761 ctgctggacc tcgaccgcct gctcagccag cggaacttcc gccagatatt caacgaccag
     5821 ggccgcttcc tcgccgccgc catcaacgac ccccagcggc aggagcagat caaaaaagtg
     5881 ctcgtggaca tgaaccgcgt gaacatggtc gtccacggcg cctcgcagat ggccgccagc
     5941 aatgccttcg gcgcctggga gcaggtggag aaggaatacc acaatttccc cgaagacccc
     6001 gagatcaccc gcctgcgctc cgaactcagc gtcaaggcca gcgaatacgt cagcgccatc
     6061 gagaaagcca ggcagcacga gcagcgcagg cagaccggct ccgccatcgc ctggtacttg
     6121 aaagcccgcc gcctctaccc gcccagcgag ttcgcccgcg acggcatcca gaacctcgtc
     6181 ggcagcctca actccgccga ctccgccccc ggcggcgccc agccatgaac cgccgccgtc
     6241 tgctccgcca gtccgcgctc gctcttggtg cctcagtcct gcccggagcg cccgcgcagg
     6301 gagggcagtt cacggggaaa atccgcaagt cgctcaaatg gagcatggtg gccgaggcgg
     6361 ggaatctgcc gctggcggag tgcttcaaga agctgcgcga atgcggtttc gagggtgtgg
     6421 agccgagcct cagccatgtg ccggatgacc gggtggatga ttggctggcc gccagcaagg
     6481 ccagcggcct gatcattgac ggcaccgtgg gcggacgggc tgccactttg gagtcgggag
     6541 tggacctgac ccggaagctc ggcggggact cgatgctggt agtgatgtcc tatgatcaga
     6601 aagcgccgct caagccacag tggaacgccg cgcgggaccg gctcaaggag gcggcacccc
     6661 atgcgcaaaa tcagggcgtg aaaatcctgg tggagaatgt gtgggcgact tttctcatca
     6721 gcccgttcga catggcgcgc ttcattgacg aggtgggcag cccctgggtg gcggtgcatc
     6781 tcgacatcgg caaccagatg cgctggggtg tggcggagca ctgggtggag gtgcttggcg
     6841 agcggtcaca aaagctcgat gtgaaggaat acgatctgaa caaggcgatg agcgagggca
     6901 tgaggaaggg cttcgacacg ccgatcgggg agggcagcat caactggcct gccgtgcgcg
     6961 cggagctggc gaagatccag ttcaccggct gggcagccgc cgaggtcaag ggtggcggct
     7021 gggattatct tgctgatgtg gcccggcgca tggaccgggt gctcgatctt gcctgaagcc
     7081 gcttccgctc actgctgcgg gcgcagatca aagcaggcca gcttttcctt ctggcgcaca
     7141 atgagccggc cgttgctcag ggcaggggag gtgcagccca tgccgccgag tttcacccgc
     7201 gccagggtct cgtattggtc ggagcgcgcg cgcatcacgc gcagatggct gccgttgttc
     7261 tcatagacca ggattttgcc atcggccagg atgggggagg tgatggtgct gttcatctcg
     7321 ttttccagcc aggtgatctt gcccgtggcc agctccacgc actggtgctt gccgccgcaa
     7381 gtcagataga catgaccgtc atggatgata gggctgccgc tgtagcgcac cgtcacccag
     7441 aaatgcgacc acgccggctt cggcgcgcca tccttctgat actggtaagc ccgcagcccc
     7501 acatccttcg agccgctgta aaccaccagc cagtcgccct ccgtcacggg ggtggactgc
     7561 ccgccgccct cgaccgtcca cttcacctcg cccgtgtcag gggccagccc gtaaaggctg
     7621 ccgctgccgt tgatgataac cactggcggg ccggatgccg gcttccacca ggccgggctg
     7681 cccgtgttgc ccttcgcggc tttttgctcc cagagcagac tgccgtcctt cgcggcaaaa
     7741 gcctgcgcat gacccgccgc catatagatc agaccgccct ccaccatggg ggacgctgcc
     7801 ggacccttgc cgggcagctc ggccttccag cgcagtttgc catcctccgc gcccaccgcg
     7861 taaaggtgcg tgctgcccgc gaagaagacc tgcccgtcca tcaccgccgc cgtgctgctg
     7921 gaactgcgcc ccgtcggctt gccctcagct tcgtatttcc aaagttcttt gccagtattc
     7981 aggtccaagc acaccagcac atccttcgcc accttgatcg tgttcggcac ggcggagagc
     8041 agtttttcct tgagcgcgtc gctgaatttc tcgtcctcca gccattgctt gaactccgcc
     8101 acactggcga agggcttgcc ctgccgcttc gacaccttgt ccagctcctc cagaggaaaa
     8161 gccgtcttgc ccgcgcggaa gcgggagacc gcccagttcg tcagcgacac ctgctccttc
     8221 tccgtcatgt tgtccttcac ccattgtttg gcccactcat ccagcttctc accgcgcagg
     8281 cgggaactca tcgaaagccg cgccgcctcc atcttctccc gcaactccgg acttgtcccc
     8341 cggtagttga gctgctgcat cacctccgag tcaatctccc gtgtctcgga gaccacccgg
     8401 tcatgccaga ccacggacaa aaacacccgc tcccctgcca ccaccgggct gccgtggccg
     8461 ccgtagtggt cgctcgggat gaagccgctc tcccacacct gggtcagccc cgcctcggga
     8521 aaaacctccg caatcggcgt cgtgtccgtc gaaactccgc cccgccccgg cccgcgccat
     8581 tgcggccagt cggcgtgcag attcggcagt gtggagagga ccaagaagaa aaaagcggaa
     8641 tggcgcatgg cagtctgcaa acgccatgcc gtgccgcgac ctttcgttgt ctcggagcat
     8701 gagtttgcgt ggtgggcgaa aatttaacta aattcatact tgaataatat agatagatga
     8761 gaaatgcata aatcgaactg gccgacccac taccccctgg aggccagttc cgcgctcatc
     8821 agtccatcac tgaagttcgc ctgtagcacc cgcacctgct gacgccgacc ggaccgccgg
     8881 aagcctatgc gccacagttg ccatgaaaac tccctctctt cccattccgc acggtcttcg
     8941 cgccacactt gtcatcatcg tttcggccat cgtccttcct gcaatctcca atgcccggct
     9001 ttactgggac accaacggct ccacgcccgg ctcaggcaat gctgctccgt ccggtgtctg
     9061 gaatctcagt gctcagaatt ggaataacga tcagggtgat ggaactccct taatctggct
     9121 caacgacggc acaggccagg cggtcttttc ggcaggttcg gatgccactg gtgcctatac
     9181 cgtgaacctg agcgcgggag agatcatccg attggccgcc ctgtcacttc ggagcggttc
     9241 tttgacactc accgccgatg ctgcggcaga tgttctggat tttggcacga tgacagccgc
     9301 cctcgttgtg gaggcgggca gtgtgctgga tgtccaaact caggtgcttg gcactgcggg
     9361 actcatcaaa tctgggacgg gagtgctcag gctggccgat gccggtccat ctggcgcttg
     9421 gaacgtcagc aacggcaggc tcattctggg tgccaaccaa agcgtgacca cgctgactct
     9481 gggcggcagt tccacttcgg tcgccgcgct ggagttggaa gacggcatcg tccttgatct
     9541 cggtggcaac atcacactga ccagcctttc acagcaggaa acagtggccg ttcaaggcgg
     9601 tgtccttcgc ctgaatggca gccgcaccat aaccattccc aatgtgcccg cgccggtgga
     9661 tatcgaaatc agctcggtgc ttgccgatgg ttcggaagtc tccagcctga cgaaatccaa
     9721 caacgccggc actttgcttc tgagcggtgc gaacacctac acgggtgcaa ccgtgctcgc
     9781 cggatcaaac tccggaaccc tgctggttca gggcaacaga gcctccatcg ccggcagttt
     9841 ctcgatagtt gtcaacggct ccaccctgac actcggtact gccctggata ccgacgctgt
     9901 gaaccgcctg cgcgacgacg cggcgatcac gctcaacggc cagacaggcg cgggagcctc
     9961 gctcaactac aacggctccg atttcgccac gcagagcatc caccaggaaa ccatcggtgc
    10021 gatcaccgtc ggaggtcgcc accgcagttt catcaccttg actcccggcg ctggtgatga
    10081 ggtggtgatt caggctgcct ccatgtcacg agcggagaat ggcatcctgt tgctgcgtgg
    10141 cagcaacctc ggtgccgcct ccggcacggc ggactcatct agactccagc tagcggacac
    10201 ctcatccctg gtgggcggcg acaacagcac gaccgggcgc gccattctcc cttgggctgt
    10261 cggagatacc agcgtggcgg gacccggcac aggattcctg acttatgacg aacaaaccgg
    10321 ccttcgattg ctggacaatg ccacagaatt tgtcgcgcca gcctctgccg gagaaggcct
    10381 taacgtgcgc aaatctgcca caggaaacct gacgctcacc agcagcgtag cccataacac
    10441 ctggactaac gcggccactg gaaccgtggc attgggcgca ggcgtgaccc tcggcttgga
    10501 aagtggcgcg gttctgttca ccgccaacgg cacattcgca ggcgggactt ttgcgctggc
    10561 ggaaacaagg caaggcatca tccatctggc cgccaacgcc gccgtcaccg caacgatcaa
    10621 cagttcactt tccggcggca acggcctgct catcagcggc acaggcacag gaaacaagat
    10681 tgtcgttctc ggcggcgaca acagcttcac tggcggcgtc ctcgtttacg ctggcatcct
    10741 ccagctcaac catccaggcg cgctcaacag cagcggtgtg aactctctcg tccttcaagc
    10801 cggtggcacg ctgcgcttga atggcaacag catcacccta tcagggctgg acggtgccgg
    10861 aaccatcgtc aacaacaacg cggcgaatgc cgccgaactc accatcaacg gcggaggcac
    10921 tcacacaggc gcgcttaaca atggcagcgc cgccgcactt gggctggtca aaacggggaa
    10981 cgccctcctg ctgctcggcg gcaacaacaa ttacaccggc agcacagtca tccagcaagg
    11041 cagtgtgcat gtgcagcccg caggcaacaa ccccggcggc aatggcggct tgcgcggcac
    11101 ctccaaaatc accatcggca agagcgcgct gctccagatc aacaacggca atgtcgccgg
    11161 gtcgaacaat gccgaccgtg tcaacgacag cgctccgctt cttctgctgg gcagcctgac
    11221 ttacaaccac tcggcctcca atctggccta cgaggaaact ctcggcgcgc tgatcctcgg
    11281 ccagtccgcc gcgcagatca ccgccagcat cgccggtgcg tccggcacct ctgtgctcac
    11341 cttcaccagc ctcgccggga gggagcatgg tggagtgttg aatttcaccg gcaccagcct
    11401 gggagctgct gacagcaacg ctgggcgcaa ccaaatcttt ttcaatagcg gcgtggcgta
    11461 cggcttcatc ggaggctggg ccacaattgg caatgagttc gccaaataca ccgcagtcac
    11521 gggcgtgacc ggctttttgg cagacgacta cttcaccggc gtccaggaag actggactcc
    11581 cgccactcat gccaaacctg acgcagcaca gatcctggac tccgaccgga acgtgctttc
    11641 tctcaacctc accggaggcg gcccgaaaaa cgtgaacctc ggaggcaacg tgctgaacat
    11701 tggctcgggc gggctcatca agcaaggcgg tagcgcgggc accacaggtg ccgctaacgt
    11761 ggccaccatc agccagggca gcatcacggc gggcggcgca gcggcggcgg agttattcat
    11821 cagggtcacg ggagcaaacc tgaacatcag cgccgccatc accgataatc tgggcgacgg
    11881 cgacacaccc ggcgcggtga cagtggtcaa aagcgggccg ggcaccgccg tgctaaccgg
    11941 caacaacatt cactcaggag gcttttttct gcacgaaggc gtggtcgtcg tgaacaacaa
    12001 cggacggacg ggcacgggtt ccgtccacgt cggccccggc gcgcgccttg gtggcaacgg
    12061 catcatcggc tctctttccc caggtgctgg gatcgagatc gaaccaggcg ggactttgtt
    12121 tgtcggtcag tccacgggac tcggagcgca gaccctgaca ttgcgggctg aaaatggttt
    12181 cctcatctcc ggctcggtgg aattggacat cgtcggtggc gcggccggcg gtctgctcaa
    12241 cccgcaaacc ggcaacaacg accggctcat tttcaccaca gctacaggcg tgggatttgg
    12301 aactcccgat ctctccggcg cgcttttgaa ggtcaacacc aacatcactc tcacccccga
    12361 cgcatggacc gtaggctcag catggaagct cttcgattgg tacggattga cctccggttt
    12421 cgacaatctg ccggacaccg gcacgcgcca tggcaacccc aatcacctcc cggacctcag
    12481 ctcactcggt ctcgcgtggg actggagcca actctacacc gacggctccc tctccatcgt
    12541 cgttccagaa cctggacgcg ccctgcttct cacgctaggc atggcctttt ctctgctgcg
    12601 caggacccga cctatgagat gacccccatg ccctccaatg accgcccttc tacttctcct
    12661 cacagccacc gtcctcggca tctctttcct ctgctcgctc acggaggcgg tcctcctgag
    12721 cctgaacccc ctccatctca agctgcagga gcgccgtgga atgaaaatgg cggcgcgctg
    12781 gctggcgctg aaaaaccgca ttgagcgccc catctccgcc atcctggttt tcaacaccat
    12841 cgccaacacc ggcctggcct ccctctccgg cgccatcttt gccgggcttt atggcagcga
    12901 atggctctgg gcctactccg tgcttatgac tttggccgtg ctctttggcg ctgaaatgac
    12961 gcccaaaatt cttggcgtcc accacgcgga gcggctcgcc ccaaagctca tccgcccgct
    13021 caccttcgcg ctttttcttt gccacccact cgtggtggtg atggaagaat tctgcaagag
    13081 gctcaaaaca agcgctggag acaagaaaag cccgagcgat cacatcatgg acatcatcac
    13141 gcttgtccag gccgcgcgcg ccgagcaact cctgcacaac cgggaggaat tgatcattat
    13201 ccacgccgcc accctcagcg cgcgcagggt aaaaacagcc atggtcccgc gcgatgaggt
    13261 caaagttttc gatgagcgcc tcaccttgaa ggagaacgtc ctagccgcag gcgccaagct
    13321 gcaccgcagc tacccagtga gcgcggacgg cacactggag cgcgtcaccg gctacatccg
    13381 cgtgcgcgag cttttcgtgc aaaacctcat ggaaaccggg ggttccgact ggcgcaaact
    13441 cattcgtcca tcactttaca tagacgccaa agcctccctc acccaactcc ttgccctcta
    13501 cctggacagg caggaggttg ccgcgctcgt tgacacacct gaaggccgca tcaccggctg
    13561 gatcaccatg gatgacgtga tgaaagtgct catgggcgcg cggatttgac tgtcgcgaaa
    13621 cccaatccct atttgcacag cagggatgag ccttgtagga tggcgaggat tcatatgaaa
    13681 ctcaccaaac gcggcgaata cgcgctccgc accctcatcc gccttggcgt tgccgcctgg
    13741 aataacgaag gcgtcatttc cgtgtcagtg ctggcagaaa aggagcggct tcccttcaag
    13801 tttctcgaag caatcctgtt tgacctgcgc aacgcgggtt atgtggaaag tgttcgtggc
    13861 aaatacggcg gcgcgcgact cgcaaagcca ctgaacgccg tgaagatggg cgaggtcgtg
    13921 cgcctcatcg acggcaagct ggcccccatc ggctgtgcaa gcgagaccga atacgagaag
    13981 tgctcctgcc cggacgaaga gcactgcggt ctgcgcatgc tcatgattga tgtccgcaac
    14041 gccgttgcca acatcctcga caaatacacg ctggaggacg tggtggaggt cacgctgcgc
    14101 aagcagctca tcaccgcgag cactgcaaaa aaatccacgc gcaaagctgg tcacgagcgt
    14161 cacgccgatc ctgccgacgg cttccttgcc gtgctgctga acaccgcccc cgcccgcagg
    14221 cgctgattcc gccgcatttt ctgcggctaa aaagatgaaa aaagcggttg ctttaattcc
    14281 tatttaacag atatacataa aatcaaagtg attgaaacct catgcagcaa gccccccaca
    14341 cccaggactg gatcgaaatc gtccgccaga aagtcgccgc catgcgcttc ggctcggtgc
    14401 agatcatcgt tcatgaaggg cgggtgacgc aggtggaaag cacggagaag actcgtctgc
    14461 ctggagacat cccgccagcc tcctccaaca gacgcgcctc cgcctgatct cggaatcatc
    14521 caactcaagc cgcccaacca cgtcatggca atacaaacca gcatcctcga aaccgtcggc
    14581 cgcaccccct tgatcaagct caacaggatc gcgtccggct tgcccgtcac catcgcgctg
    14641 aagggtgagt tcgccaatcc cctcggcagc gtgaaggacc gcatcggaag cgccatgatc
    14701 gagtcggcgg agaagtccgg catcctcaag ccaggcggcg aaatcatcga gccaaccagc
    14761 ggcaacaccg ggattgcgct ggcttttgtt gcagcggcca gaggctacaa gctcacgctc
    14821 accatgccgg agagcatgag cctggagcgg cgcaccctgc tggcgctgct gggggcgaac
    14881 ctcgtgctca ccccagcgaa ggagggcatg aaaggcgcta tccgcaaggc tgaggaactg
    14941 ctggcagcga ctccgggtgc ctggatgccg cagcagtttg aaaatccctc caatcccgaa
    15001 gtccaccgcc gcaccactgc ggaggagatc tgggaggata ctcatggcgc ggtggacatc
    15061 ttcatcgccg cagtcggcac gggcggcacc atcaccggcg tgagcgaggt gatcaaaagc
    15121 cgcaagccaa cgctggtctc catcgccgtc gagccgcagg actcccccgt catcacccag
    15181 acccgcaacg gcgagccgct ggtgcctggg ccgcataaaa tccagggcac cggcgcgggc
    15241 ttcgtgccgc gcaatctgaa cctcggcatc gtggatgacg tgatccaggt cagcaatgag
    15301 gatgccatcc acaccgcgcg gcgtctggcg ctggaggaag gcattctggc cggcatctcc
    15361 accggagcca atgtgtgggc tgctctgcag gtggccgcca gacctgaaaa ccagggcaag
    15421 ctcatcgtca ccgtcggctg cagcacaggt gagcgctacc tttcctcgcc cctcgccgaa
    15481 gaagcccgca accgcgtcca atcctaacct ctgatccacc atgaaactcc tgccccccct
    15541 caacgtccgc cgcagccacg cgccccgcgc cgcgcacagc aacctgctca ccctcgaaaa
    15601 tcacattctc gacgagggct tcttcatccc gcccaaaatc atctcgcggc cggaaaagcc
    15661 gggacagggc tacaagttcg gcgtcttcgc gggcattcat ggcgatgagg aggccggtac
    15721 gctcgccgtg caggagctgc tgaaatggag cggcgagcag cccgatgaga cccaggactt
    15781 cgagctgcac ttctaccctg tctgcaaccc cagcggccgc aacctgggca cgcgccacaa
    15841 ccaccgcggg ctggatctca accgcgaatt ctggagcggc accattgaac ccgaggtgct
    15901 ctacctggag ggcgagctgc gccgcgagcg ctacgacggt atcatctccc tgcattccga
    15961 tgacgacgcg gtgggctgct acggcttcgt cagcggcgac atactcagcg agcacttgct
    16021 cgaccccgcg cttgccgccg ccgccgcgca cattcccagg gaggtggccc ctgtcataga
    16081 cggcttcctg gccgagcgcg gcatcatcaa ggaaggctac ctcggcatcc tcagcgcccc
    16141 gcccgaacag cgcccgcgtc cgttggaggt ggtgttcgag acccccggtc tcgcgcccat
    16201 gcaggtccag gtggccgcga ccgtcgccgc tgtcaaagcc atgctccacg gttacaggca
    16261 gttgcatgcc tatgccgcaa acctttgatt tccccgcctc tgcgtgccat ctggcagaat
    16321 gcccacccgc cgccatttcc tcctctccgc cgccgccctc accggctgcc gcccggctgc
    16381 ggaccccgag ggcgtgctgc gtatcggcca cttcccgaat gtcacccatg tccaggccct
    16441 cgtcgcccgc agcatgtcgc gcgaggggcg cggctggttc gagcagcgcc tcggtttgga
    16501 ggtccgctgg ttcacgtaca atgccgggcc gagcgtggcg gaggccgtct tcgcccgcag
    16561 cctcgacgcg gcttacatgg ggccaagccc cctgctcaac gcctatgtcc gcagcgacgg
    16621 gcgcgagatg cgcgtgctct ccggggccgt caatggcggc gccgccctgc tcacccaccc
    16681 gcagtccggc atcgaaaaag ccgccgactt tcggggaaaa aagctcgcca caccccaact
    16741 tgggaacacc caggacgtgc aggcccgcgc ctggctgcgc gcgcagggct tccaggtcag
    16801 cctcacccgg cacgacgttt atgtgatccc caccaagaac gccgaccagc tcgccctctt
    16861 cgccaagggc gacctcgacg ccatctggac caccgaaccc tgggccaccc gccttgaggt
    16921 cgagtccgat gcccgcgttt tcctggagga caccaccact cccgccaccc tgctcgccgc
    16981 ccgcgccgac ttcgccgggc agaagcccga cttggccgaa aaactcgcca ctgcccatcg
    17041 cgagctcacc gcctggattc ggcaaaaccc cgctgaaacc gcagaacgcg tccgcgccga
    17101 actcgaagcc ctcaccaccg cccgcatcag ccccgagctg ctcgcccgct ccatgcgccg
    17161 ccttgtcctc accgacgaag tctcccccgc gcagctccaa accctgctca cccaagcccg
    17221 cgacgccgga ttcctacgcg gcgcgcccgc gcttgacgcc ctgctctcca cctgcgccac
    17281 tctccgcacc gactcatgac cgcctccgcc accctccttc ccgaggcccc acccgccaag
    17341 ctccgcatcg agagcgtcag caagaccttc cacggcaccc gtggcgacgt gctcgcgctc
    17401 gataccacca ccctcaccgt ggacgagggc gagttcgtct gcctcgtcgg ccccagcggc
    17461 tgcggaaagt ccaccctgct caacctcgtc gccggtctcg acacacccag cgccggccgc
    17521 ctgctcatgg acggcaaagc catcaccagt ccaggatccg agcgcatgat gatgttccag
    17581 gagcacgccc tctttccctg gctcgacgtc atcggcaacg tcatcttcgg cctcaagctc
    17641 aaagacggcc tcaacgccaa agagcgcgtc gaagtcgcca aatactatct caagctcgtc
    17701 ggcctcgagc gcgtcatgaa atccagcatc cacgagcttt ccggcggcat gaggcagcgc
    17761 gtcgccctcg cccgctccct cgcccccaac ccgcgcgtcc tgctcatgga cgagcccttc
    17821 gcagcactcg atgccatgac ccgcgagcag ctctacgccg acctgcaaaa aatcagcgcc
    17881 gcccagaaaa agaccgtcat cttcgtcacc cacaatgtcc gcgaagccgt ctgcctcggc
    17941 gaccgcgtca tcctcttctc cccgaatccc ggccgcatcc gcgagcagtt caaagtccag
    18001 ctcccgcgcc cgcgcgacat caacgacccc gccctctccg tgctcgccgg cgaaatcacc
    18061 cgcgccctca aaagccacac ctccgctccc gctgacgaat gaaacgcctt ctcatcgcca
    18121 ccgccttctt tgccctgctc atcctcgcct ggcacctgct cgtgcgcacc ggtgtctggt
    18181 cccctgtgct cctgcccggc cccgccgccg tcggcgaata cctctgggcg gctctcaaag
    18241 acggcagcct cgccagctcc tcctgggtca ccctgcagcg gctcctcctc ggctacctca
    18301 tcggctgcgc catcggcctg ccgctcggca tcctcaccgc ccgcagcaag acgctcaacg
    18361 acaccctcgg cgtgcttgcc ctcggcctcc agaccctgcc cagcgtctgc tgggtgcccc
    18421 tggccctcat ctggttcgga caaagtgaaa gcgccatgct cttcgtcgtc atcatgggca
    18481 cgctctggtc cgtcctcatc agcgtggaca acggcgtgcg caatctgccc cccatctata
    18541 cccgcgccgc gcgcaccatg ggcagcagcg gcttccacac cctgcgccac gtcattctcc
    18601 ccgcctccat gccctacgtc atcagcggca tgaagcaggg ctgggccttt gcctggcgct
    18661 ccctcatggc cgcggagatc tatgtcacac tcaccggcta cggcctcggc cacctcctcc
    18721 acttcggccg cgagctgaat gccatggacc aggtggccgg tgtcatgctc gtcatcgtcc
    18781 tcatcggact cctcgcggac aaaatccttt tctccccctg ggagcgcttc ctgcaccgca
    18841 ggtggggtct caatggctga acagacaacg cgctgcaagt gaggcagggc ggctggtgct
    18901 ccatgacttg ccctctggag gcaggagaca gttttgattt gagcctggag cattggaaat
    18961 ccagcactca aaaatgacat cccaaagcct ggtgacgcgg gccatgtcat ttggccttcc
    19021 gcagccattc tgcgcatgaa tccgtgtttg cgaagatgcg cgtggcggct ttcctgcccg
    19081 gatgaagcca atccttctgc tctccgtgta tttcgtgtgt tccgtggtca attccttggc
    19141 ccaggagccg cgcagcatca gcggcatcta tccttccctc gccatgttca acaacgaggg
    19201 cgagtgcggc acgggcgcgg tggtgccttg ggcggaccgg ttgtgggtca tcacctatgg
    19261 gccgcatctg ccgttcggat cgagtgacaa actctacgag atcacgccgg agctgaaaca
    19321 gatcgtgcga ccggagagcg tcggcggcac accggcgaac cgcatgatcc acgacgagtc
    19381 gcagcagctt ttcatcggcc cgtatgtcat cgacgcagag cgcaaagtgc gcgtcatccc
    19441 gccgagcatc atgccaggcc gcctcaccgg caatgcgagg cacctcaccg atcctgcggg
    19501 caaaatctac tacgccacga tggaggaggg cctctacgag gtcgatgtga agtcgctcgc
    19561 cgtgaaaaac ttcatccgcg acggcaaccc gatcaaaaaa gatttcaaag tcgagacaca
    19621 gctctcgaat ctcgaatctc aactgcccgg ctaccacggc aaaggcctgt attcatctca
    19681 aggccgcgtc atctacgcga acaacggcga ccgcgacaag cgtgtgctca ccgatcccac
    19741 cacccccagc ggagcactcg gcgaatggac cggcagcggc gactggcagc tcgtgcggcg
    19801 caatcaattc accgaagtca ccggccccgg cggcattcac ggcagcgatg caaacgcccc
    19861 cgtgtggagc atcggctggg atgcaaagtc gctcatcctc atgctgctcg atggcggcaa
    19921 atggcagtcg tttcgcctcc cgaaagtcag ccacagctac gatggcgcac acggttggaa
    19981 caccgaatgg ccgcgcatcc gcgacatcgg cgaagaaaac ctgctcatga ccatgcacgg
    20041 cgcgttctgg agcttcccga agaccttttc cgcatcaaac acgaacggca tccgcgcccg
    20101 cagcgcctac ttgaaggtca tcggcgactt cacacgctgg aacgaccgcc tcgtcttcgg
    20161 ctgcgatgac tccgcaaagt cggagttcct caacaagcgc caggtcaaag gcggcatcga
    20221 cagcatcggc cagtcgcaga gcaatttgtg gttcacgagc gccgacacgc ccggaaagct
    20281 cggccctgcg cacgccagcg gcgcggtgtg gctgcatgag agtgtcaaag cgggtaatgt
    20341 gagcgatccc ttcctcgtcg ccggttggaa gcatcgcagc gtgtggcaca tcgaccatgc
    20401 caccggcgaa tcgaagcgcg tcgagatcag cagcgatgac gaatggctgc gtgtgaaggc
    20461 cgcaagcgac tccaaagact tctccgtgct catcacgctc tcgaatggcg attcacgctc
    20521 caccgagccc gacgcgattt tcgacggcat cgcccgaatc ggcgatgaaa atgcccaaac
    20581 cggccttttc cgcccgcgcg gcgaaaacaa gcgcacgctg agttttgcga cgaaagacgg
    20641 ctactacgag atgggagccg atttggccct gaaacgcgtc gaggatgccg cagcgaaggc
    20701 ctttctcgaa agagccgtcg ccctgccgaa gaacgtcatc acgctcgatg aggccagcgt
    20761 gctcgtcgtg gatgatcgcg gccgtcgctg gcgcttgccg cgtgcctcca aggcctacga
    20821 tgccgccacg aaggccggcg aactgcgcat ctgccgcgag gtcgccaccg agcgcgacat
    20881 gctcagcgcc tgtggcacct tcttcgagct gcccgccgaa aacgccgacg gcttcgcgaa
    20941 gatccgcccc atctgctcgc acgatctgcg tgtgaccgac ttcggcggct atcgcggcct
    21001 gtttttgatg agcggcatca agccggacgc caaagctggc gatcacatcc tccgcagcga
    21061 cgacggcaaa gccgccctct gggccggagc catcgacgat ctgtggaaac tcggcaaacc
    21121 gcgtggtgaa ggcggcccat ggaaagccac gaaggtcaaa gctggccaaa aaagcgaccc
    21181 ctacctcatg tgggcctacg accagcgcac gctcacactc agccacgatc aaaacgagcc
    21241 agtgagctac cacatcgaac tcgacctcac cggcaccggc ttgtgggtaa cctggcaatg
    21301 gcgcgaggtc tctcccggcg aggatgccct gcttgtgttc gaccctgcgc tccaggcgcg
    21361 ctggctgcgc gtcaccgcca gcgaggactg cacggccacg gcgcagttgc gccacgagtg
    21421 agcacggcac ggcgcaagcg tcccgcggcg ggctgtgatg aaaatccgaa tttccttttt
    21481 gacgcaaggg cgagcggcgc gcacatcatg atgatgcgcc gttatttcat cccccttctg
    21541 tcatgtgtcc tggtgcaggc cgccgccgcc gaggtggtgg cgcgcaagtg gtccgggact
    21601 ctgaatgtgc cggacccggt ggcctgcacg gtggatgaat acggccgcgt ctatgtggcg
    21661 gcgacgacac ggcgcaaggc ggcggatctg gacatccgcg agcatccgat gtggatcgct
    21721 gatgatgtgg gcctgacgac ggtggaggag aagcgggagt tcctgaagcg cgagctggcc
    21781 cccggcaggc tgcgcctgcc gcgcggcggt ctcaaggatc acaacaagga cggcagcatt
    21841 gactggcagg acctgacggt tcacagcgag cgcgtttacc agttgcggga cacgaacggc
    21901 gacggcttgg cggacaagat gacggtcttc gcggagggtt ttaacacgga ggtcacgggc
    21961 atcgcggcgg gcatccttta tcatgacggc tgggtttacc tgacctgcgc gccggacctc
    22021 tggcggctga aggacacgga cgacgacggt gtggcggatg tgcgggaggt ggtggcgcac
    22081 ggcttcggca tgcacatcgc ctacgcgggc catgacatgc acgggccgcg cctggggccg
    22141 gacgggcgca tctactggag catcggcgac aagggcgtga gcgtgaccag caaggagggg
    22201 aagaaattcc acctgccgca cgagggcgcg gtgctgcgcg tggagccgga cggggcgggc
    22261 ttcgaggtct tcgcccgcgg cctgcgcaat gtgcaggaga tcgccttcga cgacttcggc
    22321 aacatgtttg gcgtggacaa cgacgcggac ctgcccggcg agcgcgagcg gctggtttac
    22381 atcacggatc aaagcgactc cggctggcgc tgcggccatc aatacatgaa gggcgcgagc
    22441 cgctggatcc tggaggacgt ctggctgccg cagcagccgt ggaaaggcgg cggcagcaaa
    22501 cagccgctgt tcatcacgcc gccgctggcc aattacagcg acggccccgc cggcttcctg
    22561 cacgagccgg gcacggcgct gggcgcgtcc ctgcgcgggc atttcctgct caaccagttc
    22621 ccctccggca ggatggacgc gttcaagctg gagccggacg gcgcggcatt caagatgacg
    22681 gacccgcgca ccatccacaa cggcgtcatg ggcatcggca tggcctgggg cgtggagggc
    22741 gcgctttact tcgccgactg ggacgggggc tatccgctgg atgagaaagg cggcatctgg
    22801 acgctcgact cggcggcgga caaggacgct gccgcgcgca gggagacgat ggagatcatc
    22861 gccacgggat ttggctcgcg cccggactcc gatctgcaaa agctgctcgg tcatgccgac
    22921 cgccgggtgc gcatgagcgc gcagctcgaa ctggcgaaac gaggccagtg ggacgcgctg
    22981 ctcgccatcg cggcggacac gggcgcgccg ctgctggcgc gcgtgcatgc catctggggc
    23041 gcgggcatcg gcctgcggca cggcaaggtg ccggagacgc tgttgcatga gcgcctggcg
    23101 gcggagacgg atgccgagat ccagacccag ctcgtcaaaa tcatcggcga cgccaaaccg
    23161 agcgtggccg ccacccgcgc cgtggaccgc atgatgacga gcgaatcccc gcgcgtgcgc
    23221 ctgcacgccg cgctggcgct gggccgcatg gcgccggttt ccgccggcct gtccctggcg
    23281 aaggtggagc tgaacaacgc cgccttttac ctcctggacc gcgtcgagcg ggacgcgcac
    23341 gatcccttcc tgcggcacgc cctggtgtcc ggcctggcgg gctgcatgag cgcggaggac
    23401 ctcgcctccc gtttcgactc cgagtccgag cccgtgcggc tggtctgcgc gctggcgctg
    23461 gcgcgccagc gctcgccgct catcaaggac tacttgaagg actcctcgga cgccgtcttt
    23521 gatgaagtgt gccgcgccat tcacgatgac ggcgggattc ccgatgtgct gcctgagctg
    23581 gccgccctgc tcgatggcgg acggccgctg actccgcccg cgcgccgccg cgccatcaat
    23641 gccaacctgc gcatgggcca gccggtgaac gccgcgcgcc tgctccagca tgcgttgaac
    23701 cagccgccgg agcaggcggt ggaggcgctg cgcacgctgc tggtcttttc gaagccgccg
    23761 cggctcgacc tcgtggacgg catggcgggc acttacccgc gcatggatga gaacgccctc
    23821 tcgcccagcc tgatcgaaac ctggaaaagc gatctgctcg cgctcaaaga cccctcgttg
    23881 aagacggcgg ttgtcgaact gctcatccaa ctgcaactcg aggtggacgc ctccgtcctg
    23941 ctcgccatcg ccgtggatga aaaggcggag acaacgctgc gcatcggcgc gctgcggctc
    24001 atgaccgcca aaaacccgca ggcgcccgcc gtggcgcagg cgctggaaaa gacctccggc
    24061 aagaccgcgc ccgaggagct gcgcatggag tcgctggcgc tgaccgcgcg ccaccagccg
    24121 gagcgcgcgc tgggggaaat ctcgcgcgtt ctggacagcg gcaccctgcg cgagaagcag
    24181 gccgccattt ccctcctggc cgcccatccc ggcagcgggg cggacgcgct catgacggca
    24241 tggctgcaaa agctggctga tgaaaaggtg gagaccggac tgcggcttga tgtcattgag
    24301 gccgcgcagg gccgcgaatc cgtcgcggcg cgtgtcaccg catggcagca gacgcgcaag
    24361 acacccccgg cggaggattt gatcgagggc ggcgacatca aggcggggcg cgagatcgtc
    24421 aacaaccaca tcggtgccaa ttgcgtggcc tgccacaccg tcgaggcgaa ggagggcagc
    24481 caggtcggcc cgccgctcaa gggcatcggc actctgcgcg cgcgcgcaga gctgctggag
    24541 tccctggtca atccggtggc ccagatcgcc gagggctacg gtctggtctc tctcacgctc
    24601 aaggacggcg gcagccatgc gggctcgctg gtcagcgaga gtgacaaggc cgtgcgcctg
    24661 cgcctgcccg acggcgcgct caaggagata ccccgcgaaa tcatcctcgc gcagacgccg
    24721 ccggtctccg tcatgccgcc catgctcggc atcctcaccc cgcgcgaggt gcgggacgtg
    24781 gtggcctacc tcgccagcct gacgggcgcg cctgacaaga aaccccagcc tgccaagacc
    24841 gcgccgaaag ccgtcgtcaa gacggcgccc gcgcccgcga agaaagcgcc cgcctccact
    24901 cctgctaaat ccacccccgg cgcgccgaaa gccgccgtta aagccgcgcc ggagatcgcg
    24961 ccgccgtccg tgccccagcc cgctccagcc gcgccggcac ccgcctcatc catgccggtc
    25021 gaactcgccg acatacccag ggccatcccg gtggatgaca acggccaggt catgacggag
    25081 ccgccgaagc aggagccgcg caaaggcggc atcaccctcc gctttggcaa ccgcaagaaa
    25141 gaggaggccc cgcctccacc acttgtcatg ccggagccga tgtctgccga tgatgccggc
    25201 gagacgaagg cggaggacaa gggtgaaaac aaaccgcccg ccccggcccc cgccaaagag
    25261 gccgcgaagg aaaccgccaa agccggggcc aagtccgagc ctgccgctcc cgcgcccaag
    25321 gcggaggcgc ccgcgaaggc ggacgccaag gacgccgccg ccaagtctga cgcgccgcct
    25381 ccggctgaga tgcccgccga ggcgaagacg gaggagaaaa agggattctt ctcccgcttc
    25441 cgcagcaaga agaacgagga ggagtgaggc gcgcgcgggt ctgcc
//
LOCUS       DAOMYJ010000055        25270 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Prosthecobacter sp. isolate AMR_MDS_5629
            NODE_711_length_25270_cov_22.353480, whole genome shotgun sequence.
ACCESSION   DAOMYJ010000055 DAOMYJ010000000
VERSION     DAOMYJ010000055.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867850
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prosthecobacter sp. (wastewater metagenome)
  ORGANISM  Prosthecobacter sp.
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Verrucomicrobiaceae; Prosthecobacter.
REFERENCE   1  (bases 1 to 25270)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 25270)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.30
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:34:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,742
            CDSs (total)                      :: 4,684
            Genes (coding)                    :: 4,676
            CDSs (with protein)               :: 4,676
            Genes (RNA)                       :: 58
            tRNAs                             :: 55
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25270
                     /organism="Prosthecobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_711_length_25270_cov_22.353480"
                     /isolate="AMR_MDS_5629"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1965333"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1117
                     /locus_tag="PLP58_07865"
     CDS             <1..1117
                     /locus_tag="PLP58_07865"
                     /inference="COORDINATES: protein motif:HMM:NF019011.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="M81 family metallopeptidase"
                     /protein_id="HRJ08466.1"
                     /translation="ARPEAELARRVREVAGAKAVIAGTFDPHGNEDAEFLKHADLAFA
                     YKYYPHYDAHLQGERAARMMIRAIRGDYRPARAVRTVPILSATVYQWTGQHPWSTLVQ
                     RCLTWEAREPDVYVNFFYGFPWADVPDAGMCFQVITNGDAALAKTVADDLADTAWRMR
                     AEMFSATPIHPVAEAVGQVKKALEQGRAPAVLADYSDRSGNATWVLREVIAQDLAKVV
                     IATIKDDRALNALARAGAKQGDAFDMDVGGYVDESAGQPVRVKGVIEHLSSDGKGGVK
                     AARIRFGRGNLLMLSPQLVQYMDPQSIRKDGVDITQFEVFAIKSRVHFRRGFDDNGFA
                     KTILLVEPPGPFMGTVHLDALRYENLRVREFYPFSEK"
     gene            1159..1974
                     /locus_tag="PLP58_07870"
     CDS             1159..1974
                     /locus_tag="PLP58_07870"
                     /inference="COORDINATES: protein motif:HMM:NF024627.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HRJ08467.1"
                     /translation="MFFLLLLLASAQVRAQEAPPLQEWIHEAVKAGGGVVTVPDGEHV
                     FTETLVIEDAKKLALRGMGREGCVLRMKDAAKPVIEIRGEVERVEIAGIIFQGGAGVR
                     VRGGKDVQVRDCIFEGPGVVMLGARESGVERCSFRDGKGAAVVLDESARQIVIRGNQV
                     TRMALAFDLRAAAECVLEGNEVRDCAAAIRIAGLEPAGAQKHVVRDNGFFDIGGDALI
                     AAAGTREILIEGNEVDQTQGFGFVIAGEGHVLKANTLTGTQAGQMRDERADKP"
     gene            2012..2686
                     /locus_tag="PLP58_07875"
     CDS             2012..2686
                     /locus_tag="PLP58_07875"
                     /inference="COORDINATES: protein motif:HMM:NF024863.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDSL-type esterase/lipase family protein"
                     /protein_id="HRJ08468.1"
                     /translation="MMMKPVLLLLFLASLAPAADPTPRPNPARFAKDIAKFAAERPEK
                     GGIVFTGSSSIRLWTDLKQDFPDLPVLNRGFGGSVANDLSVYFETVIARHEPRLLVTY
                     TGSNDLNAKLTPQEALADYTAFLDKFHARFPAARVILTSVKIGEKRLAQIPQVHELNR
                     MLQAWTADKEWARYLDCDSYLADDKGHPIRQYYRDDLLHLSPEGYAEWKKILEPVLRE
                     EWAKVR"
     gene            2897..3115
                     /locus_tag="PLP58_07880"
     CDS             2897..3115
                     /locus_tag="PLP58_07880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ08469.1"
                     /translation="MKKTSRLIEKSSTLKEKPPRLMDSQSPAAENPSRAHEKASPTAD
                     FSSRAIENPPSVMKIQSTAVKNQSLLTD"
     gene            complement(3212..3448)
                     /locus_tag="PLP58_07885"
     CDS             complement(3212..3448)
                     /locus_tag="PLP58_07885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ08470.1"
                     /translation="MNGKIRPLQMGCEGFFSITGFFISGADSGIERAGKADAARRNIP
                     DAQELQALLTPWFERSAAAPEAPPVEEYAPAPQV"
     gene            3533..4204
                     /locus_tag="PLP58_07890"
     CDS             3533..4204
                     /locus_tag="PLP58_07890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ08471.1"
                     /translation="MRTTAILILICGSVLLAQDREIDPFADASNSTLPAKASTPPKTE
                     DLRPSAADLKLLGEPSLEGRTDADRFPTIRVIWCRTFHPPVSLRAYLTKDGPRLRVAR
                     MSGKGGYDWGTLDFENDFPLRAETWMRLLNLLNADGARQPLKNVKQEDMDYLTGLDGS
                     TWKLEVSDQAGYTVDEVWTPMNVTNGDPDIKKLAEDQGIRLDNFVKLCSLLIQYAPID
                     PESIY"
     gene            4270..4485
                     /locus_tag="PLP58_07895"
     CDS             4270..4485
                     /locus_tag="PLP58_07895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ08472.1"
                     /translation="MNAINQLIEIGGAVFFLLFGLWATAYAYGFVGEKLAGSFCWKPE
                     FKHALRWLGPLLVLLCCASLVFLFTQR"
     gene            4547..4786
                     /locus_tag="PLP58_07900"
     CDS             4547..4786
                     /locus_tag="PLP58_07900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009958648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin-antitoxin system HicB family antitoxin"
                     /protein_id="HRJ08473.1"
                     /translation="MSSLSLRLPDSMQRHLKRAADADGISINQFISLAVAEKLSALQT
                     YDIIAERAKGASRESFLQVMSKPRPRSVYEDSTPG"
     gene            4831..5040
                     /locus_tag="PLP58_07905"
     CDS             4831..5040
                     /locus_tag="PLP58_07905"
                     /inference="COORDINATES: protein motif:HMM:NF021253.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="addiction module protein"
                     /protein_id="HRJ08474.1"
                     /translation="MTQVTALLKEASKLDLPDRAELVTSLLEDLDPEPHDVSDEEVLK
                     RLEELKSGKVKGISKEEFWKACGRP"
     gene            complement(5440..5772)
                     /locus_tag="PLP58_07910"
     CDS             complement(5440..5772)
                     /locus_tag="PLP58_07910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018971009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine/purine nucleoside phosphorylase"
                     /protein_id="HRJ08475.1"
                     /translation="MSAIPTEFPGVTAVTKANVYFDGRVVSHTLLFAGGGKKTLGLIY
                     PGAYHFGTDKAERMEIVAGACTVKLDGGESTAAYEAGAHFEVPAKSGFDITVTSGICE
                     YICSFIEA"
     gene            complement(6005..8302)
                     /locus_tag="PLP58_07915"
     CDS             complement(6005..8302)
                     /locus_tag="PLP58_07915"
                     /inference="COORDINATES: protein motif:HMM:NF038032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CehA/McbA family metallohydrolase"
                     /protein_id="HRJ08476.1"
                     /translation="MKTLWGSCLVLVASLHARDPEPQPFLASTGRLIEAMEFLGTPFN
                     SDELHTLRACIQANDADAVEKARALLDSHALFHIHINPEQRVKVQPGAASPELDEAGW
                     RQFLVRVVNEAGTTAPLRVSSPQAGPVHERPKRPNDPIPEAAPPYSARWADVQMFDAP
                     PLRPTLSGLELEYRIIQIYSRDTGKREATFTFDTGQGTQDLGFRGETSILFSCRQAHE
                     ITLRVTDEAGKPCIASLLIRDRAGRVHPSQIKRLAPDFGFQQQVYRGDGESVRLPDGE
                     YEIVFQRGPESLPETRPVTVDAATKEWSFQVKRWIDPAARGWWSGDHHIHAAGCQHYA
                     SPTEGVHAPDMARHCQGEDLKIGANLTWGPCFDYQKQFFTGAEDKVSRYPFLLRYDIE
                     VSGFGSHRSGHLCLLRLREQMYPGGDSMDHWPTLGLNTLRWAQKQGAVCGPAHSATGL
                     QVPTDELPNYLIPPFDGIGANEYIMAVTHEVEGPDGKPVPAVDFMSTADTAPHWELNI
                     WYHTLNAGFRTRISGETDFPCIYGERVGMGRSYVKVDGRLTYDAWCAGIEAGRCYVGD
                     GRSHLMDMRVNDLPLGEKGSELRLDAPATVTVRADVAALLPEQPDETLRGQKAPPNTA
                     DLPMGRRYDAFYRKPYWHLERARIGDTRTVPVEVIVNGYPVAKKVIPADGSEREISFE
                     VPIEKSSWVALRIHPSSHTNPVFILVGGKPVRAGKRSLQWCLDSVDRCWESKKDTYAE
                     AEMPDAIAAYEHARRVYRQRLAEAE"
     gene            8422..8730
                     /locus_tag="PLP58_07920"
     CDS             8422..8730
                     /locus_tag="PLP58_07920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009958443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF485 domain-containing protein"
                     /protein_id="HRJ08477.1"
                     /translation="MTENQMKVDWAALEAKPEFRALLARKARFITAASVFFVVYYFSL
                     LVLVGWFPDLMKKEVLGRINLAYLFALSQFFMAWGLAWLYTRKAAQWDLEAANIVRGH
                     "
     gene            8772..10382
                     /locus_tag="PLP58_07925"
     CDS             8772..10382
                     /locus_tag="PLP58_07925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009958444.1"
                     /note="Members of the Solute:Sodium Symporter (SSS), TC
                     2.A.21 as described in tcdb.org, catalyze solute:Na+
                     symport. Known solutes for members of the family include
                     sugars, amino acids, nucleosides, inositols, vitamins,
                     urea or anions, depending on the system; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015370 - solute:sodium symporter activity
                     [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/solute symporter"
                     /protein_id="HRJ08478.1"
                     /translation="MTIAVWMFLAFVVATLGITVWAAKKNTSAGAYFAAGRSITGWQN
                     GLAVAGDYMSAASFLGISGMIAFYGYDGFMYSVGWLVAYLTVLLVVAEPLRNAGKYTM
                     ADLLAYRLTPRPVRAMASLSTITVSTFYMVAQMIGAGALVKLLLKDLPWITTEWAIIG
                     VGVLMVVYVVFGGMLATTWVQIIKAILLMSGTILLSVLVMKHFGYSFTAFFEAIANLT
                     YKDASGAEVTRNFLEPGMKFGAAVTKGWGPLDQISLGLALVFGTAGLPHILVRFYTVP
                     DAKTARVSVVWAMIIIGAFYILTTFLGFGAATILKPDSIPTDNMAAPQLSEALGGPVF
                     FAFISAVAFATILAVVAGLTISASASFAHDFYTNVLHHGKEHRPGEEVRVARITAFVV
                     GAVSIFLAIKLQNVNVAFLVGLAFAVAASANLPVIVLSIFWKKFSTAGAVLGLAVGLV
                     SSIVLIVLSPSIMGVDGPEVAAAKRHLIQADAIFPLTNPGIVSIPLGFLAAILGSLWR
                     REPEAEAKFCELKVRAHTGLGAEKAVSH"
     gene            complement(10540..11868)
                     /gene="hflX"
                     /locus_tag="PLP58_07930"
     CDS             complement(10540..11868)
                     /gene="hflX"
                     /locus_tag="PLP58_07930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009960518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HRJ08479.1"
                     /translation="MFDIREKPQVVRSALLVGAYFDRRHSTGAAELLEELQELVRTLG
                     IGIVSADLVFAREHTARHLIGKGKAQELMAKAKELECDCIIFDNELSPAQQRAWEQES
                     GIAVIDRHEVILDIFNMRAQTREARLQVELARLEYSIPRLTRMWGHLDRQGGSGGGAG
                     GGAARGEGETQLEVDRRLAYKRLDKVKADLEEVKKQRDTMRKERSRVPVPHAAIVGYT
                     NAGKSSLLNKLTEAEVLAEDKLFATLDTTTRRLELPDGQPLLLTDTVGFIRNLPHDLV
                     QSFRATLEEAVLADFLIHVVDASSPRALEHYQTTTQVLAELGAGEKRVLLALNKTDLM
                     DDARQMELRRRFPEAVFISTRTGSGLDDLFHRLHDMLLDRVVRLELSIPLERMDLVAL
                     AHEQGKVLAEDYERGVADIQCVVPRQFESRFAPFLRREGKPAKPDKPLTK"
     gene            11946..12449
                     /locus_tag="PLP58_07935"
     CDS             11946..12449
                     /locus_tag="PLP58_07935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ08480.1"
                     /translation="MTRRLAILSFLSALASCGPVDRDGARLSTYENDATEALVREMLR
                     TLPDLNPGVPKSHSISLGEIVPKRDFTPASVPFFKRFEDTGLRIVSAAVLTTTPPDNI
                     IIDPELRVAAYIIQIRHMKQTMPGRWEYEAGWSYKKQFQRKKWTVTAANGSYKVEAGE
                     ILDGNLE"
     gene            complement(12520..13689)
                     /locus_tag="PLP58_07940"
     CDS             complement(12520..13689)
                     /locus_tag="PLP58_07940"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024871.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HRJ08481.1"
                     /translation="MEASLSSSGTLSQLFKAGGGFVPCPRARTETSRHVTEVIEEEEG
                     LARLEPFWDKLLEKSATRSPFLRWDWIWTWWRHFGEGRKLAVIVVRGHRGEVEAIAPF
                     MLSKGDHGLRRHLRLLTWISGQGPVLGERMDALVPLGREAELGPLLSDALTHLPGHAC
                     HGVWLPMIPAESPNLDWLMKAVKSVCAGAGVAEKLESRWLKLPNNWEEIEPGSQGRWR
                     RRMRRKNIIFLQKHEGRRCEEGGGGAMDELARLHAFHWPKGRSHFVTDGAWGFHRELG
                     ARWLKSGRALMPSLATPERGMVAALYGFAEGDEFSFYQHGWDPALARLSPGNLVLNWS
                     MELCSRRQMRIFDMLPGDQPYKAEWCRESRPLVSLEGFHPWNPKALLARWARKMS"
     gene            complement(13788..14882)
                     /gene="pdhA"
                     /locus_tag="PLP58_07945"
     CDS             complement(13788..14882)
                     /gene="pdhA"
                     /locus_tag="PLP58_07945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007412603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045250 - cytosolic pyruvate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0004739 - pyruvate dehydrogenase
                     (acetyl-transferring) activity [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring) E1
                     component subunit alpha"
                     /protein_id="HRJ08482.1"
                     /translation="MATKNATPASSSEAPINAKLTADDKIELYRKMARIRRFEQVSLQ
                     HYQAGRMGGFLHLYIGQESVAVGCVSLMGENDHVITAYRDHGHALAVGMGMNECMAEL
                     FGKATGCSRGKGGSMHYFAPDKNYWGGHGIVAGQTPLGLGLAYGLKYRGLKGAALCFL
                     GDGAVNQGAFHESLNLAALWDLPVIYVIENNGYSMGTSQARSSAFDGCLAKRAEGYGM
                     AWDKFVGENVYDVRAGVQKAIERAHNESKPTLLEIDTYRYLGHSVADARHQKTEDKDG
                     YRTKEEVERYQREHDPIQIWKAVLLSEGVATEDQLKKIDREAQAEADESAEFAKSSPY
                     PEPESIFEDVYWEVDNKTTAGETGRHFFND"
     gene            15044..16954
                     /locus_tag="PLP58_07950"
     CDS             15044..16954
                     /locus_tag="PLP58_07950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009957883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA];
                     GO_process: GO:0030488 - tRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HRJ08483.1"
                     /translation="MSASLYTFPKHYDVIVCGAGHAGVEAAMAAARLGCQTAILTQNL
                     DTISQMSCNPAIGGLAKGHVVREIDALGGVMARNTDATGIQFRMLNARKGPSVQAPRA
                     QCDKKAYQFRMKWLIENQPNLDLHQGNAAEILVENDTVTGIRTSLGMIYRAKAVIISS
                     GTFMRGLLHVGLQNQAGGRMGDSISTLSDSLRELGFEVKRFKTGTPCRINSRSIDFRQ
                     CEPQPGDSPAPPFSYLSGVVPEDEENTEIRNSKFEIQPSFHHFTLNSWLPEKFHVEQI
                     PCWITYTTPQTHDIIRANLDKSPMYCGRIEGVGPRYCPSIEDKVVRFAEKDRHQIFLE
                     PEGRHTREFYINGVSTSLPYEVQLDFIRSIPGLERAEIIRPGYAVEYDYCPPTQLLPT
                     LETKRVSGLYFAGQINGTSGYEEAAGQGLLAGANAALKVQGRPPFILPRSAAYLGVLV
                     DDLVTKGVTEPYRLFTSRAEYRLLLRQDNCDLRLTPLAAQIGLACPTRTRLTQEKAAR
                     LAEAMTFLQTTTHDGLRLEKWLRRTGNDHTTLPAELRAAFPDAIWQLVENDVKYEGYI
                     QRQEMMLERTARMEEKEIPAHFDYAAVHGLKLEARHRLSQIRPATLGQAARIQGVTPA
                     DIALLAIMLRRG"
     gene            17009..17296
                     /locus_tag="PLP58_07955"
     CDS             17009..17296
                     /locus_tag="PLP58_07955"
                     /inference="COORDINATES: protein motif:HMM:NF016875.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HRJ08484.1"
                     /translation="MILSFADREAEKIFRGQVSRKLPREIQDSAREKLMLLDAMVRIE
                     DLWAFPSLRIEKLQGKRAGQWSIRINKQWRICFFWDGEKVTVTGVEITDYH"
     gene            17300..17617
                     /locus_tag="PLP58_07960"
     CDS             17300..17617
                     /locus_tag="PLP58_07960"
                     /inference="COORDINATES: protein motif:HMM:TIGR02607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HigA family addiction module antitoxin"
                     /protein_id="HRJ08485.1"
                     /translation="MKTTPAKESPTRLASVHPGRILKKEILEARALSQTQLAAATGLP
                     VSRINDLVKERRGITVDSAIRLSKALGTSVDLWLNLQRAFDLEEAQRLKGEEYQRITP
                     LAA"
     gene            17631..18608
                     /locus_tag="PLP58_07965"
     CDS             17631..18608
                     /locus_tag="PLP58_07965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ08486.1"
                     /translation="MHPAATLLTLVICLSMSFASRAKDPMPVAPFLASHITSLEIRQL
                     PFPEAVEALRQAVATEEWVGAEVQIARYREPMQSEAHPWRDEYENLVSVHFRDISVYD
                     ALQALCAACGFEMDVEVFKLRCFETSGQVGRPAFEKLFPEAAQRADQLRKLKIPAFQG
                     DGLSAYEAMKKLQDEVCKLTGRQPPPFVFISQRDRKSPGKIGDDTHHPIHLNLKDTPT
                     PYFEICRYIAELSAHSFRMSADGHVILRRVADIYDPVRFRVLPVELFARQLGKPVAEV
                     TEEAMQAWMEEAGWSVYFLSYHRPSGRVFIEGLWAGDEGALLEALEKRP"
     gene            19068..19769
                     /locus_tag="PLP58_07970"
     CDS             19068..19769
                     /locus_tag="PLP58_07970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ08487.1"
                     /translation="MRILARYFDNPFDDPGISLAELLAFSTDHLGRLRARNAQGEFAE
                     PVAALESALAGLNEASMQDFSNLGLRKARKRAKRDFRRKLLPGALEKVDVAVLAFLEG
                     GRNVRQRAFPQGRTIFRTCADDHLRAHLRVMDEVVQEHAAALPPAIVELSAGLLAQWQ
                     EIYSGSESSTGAKAAAEVAQRQARAVLQGVLFDNLLLLARRHAREPDRLADYMQESLL
                     RDRVRRVRKGAAAEP"
     gene            20072..20938
                     /locus_tag="PLP58_07975"
     CDS             20072..20938
                     /locus_tag="PLP58_07975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009961176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRJ08488.1"
                     /translation="MSDPQPITPAVSVENLTKVFRGALGRKPFVAVRDVSLTVREGEV
                     YGLIGPNGCGKSTTMKTVLGLLAPTRGRTFVFGRSSTEVASRQAVGFLPENPYFYKHL
                     NGLETLLFYGRLCGLGGAELRDRAREMLALTGLEDAADRRVAGYSKGMLQRLGLAQAL
                     LHRPRLVVLDEPTAGVDPAGSRKIRDLILGFRGQGITVLVTSHLLEQMQEVCDRVGIM
                     AHGRMMREGRVDELIAVENQTELVLEDATPELLAEIRALVERQGGARLVRAGQPRTTL
                     EKLFLEATEDTL"
     gene            20938..21816
                     /locus_tag="PLP58_07980"
     CDS             20938..21816
                     /locus_tag="PLP58_07980"
                     /inference="COORDINATES: protein motif:HMM:NF024091.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HRJ08489.1"
                     /translation="MNHAAFSPARVWTLAAATVTQLVRMKILVFLLLFCAVVVAAGFA
                     FPVLNPEQQLKLLKDVSFGALQIFSLVIGIVATALLLPRDLEDRTLYTILSKPVPRHE
                     YLLGKLLGVLALIGAGLLVMDAAFSLVLWLREKLLLAQILAGLKAQTPEAVAQVQELV
                     GKQGLTWNLHLGVLAVFLKAAVVTALALMVSCFASSTLFTVVVTFCIVIIGHGQGLLR
                     EYFLQGKMEPFVEKGLSALLAVLTPDLGVFDVIDNVVNGQVVGPGSAGYMTGIALTYI
                     TGYMVVSHLLFVEKEL"
     gene            21816..22586
                     /locus_tag="PLP58_07985"
     CDS             21816..22586
                     /locus_tag="PLP58_07985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ08490.1"
                     /translation="MKRPLQALLVLLLLGALKLPLEQAATAHLRAEKLLTPRVDLGLR
                     ENLGQMGFAASLGGLRSLVASITYLRACGAWENVRWGAVDSLFQLTTRLQPRYTHYWD
                     EAAWHMAWNASSHYLNNEKLNPALREKLSYEHVLRGRDILLEGLRMIPDDARLWHTLA
                     ELYERRDIQPAKAAECYLKVGELTGNLRFRRLAGYQYAKAADRALQLKGYELLMESYR
                     ANQRPPSLINTIKDLELALSIPAMLRIPEARVAPGAVR"
     gene            complement(22598..23626)
                     /locus_tag="PLP58_07990"
     CDS             complement(22598..23626)
                     /locus_tag="PLP58_07990"
                     /inference="COORDINATES: protein motif:HMM:NF014893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HRJ08491.1"
                     /translation="MSDLHPLRIGIVGAGGIVKQRHLPGLRALPGISITAVANSTLAS
                     AETFCHDHAPEARPHSRWEDVVDNDDVDIVWIGAHPCLHHDATCFALERGKHVFTQAR
                     MAATLAEAERMWEKSRSFPELVTAICPAPHGMRGGLMVKKLLAEGAIGVPLHALLHSF
                     NSDWLPATQPAHWRQRTEISGIQILTLGIYIEVLQHWLGHITEVEARGKVGIPVRGAY
                     EVGTPDFLHVNAEFSGGLEAALLFSGVAAHAPTDKLWIFGSEGTLVYDFATEELALGK
                     TGGSLEPVAVPQEMQRAWAVERDFIGAVRNPSAPRPRPDFTEGLCYMRVVDAVWRAME
                     TGAAARCV"
     gene            23727..24434
                     /locus_tag="PLP58_07995"
     CDS             23727..24434
                     /locus_tag="PLP58_07995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ08492.1"
                     /translation="MKLASLLLLTAVSAAPAADEKPLLAIPGKVIYENKLDTAPGAPW
                     KIAKGKWELVDGVMRGSELEADNHGAVARLPNQLSNFIIEYEFKLEGARSTSLSINAL
                     KDHMARIAITPRSVTIQRDDNDHEGPDKAVVFARFPAELSPGVWHKVRLEMVDDKMLG
                     QVGELTAWGTSELFTTPKAAPGFTVSGESVDFRNLVIREATLNPAWESVMATLPKPGE
                     KVAPAPAKGKGKGKKKE"
     gene            24497..24784
                     /gene="gatC"
                     /locus_tag="PLP58_08000"
     CDS             24497..24784
                     /gene="gatC"
                     /locus_tag="PLP58_08000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018968609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HRJ08493.1"
                     /translation="MSSPEINIHKVATLARLALSDEEAARYQEQLGQILHYIDTLSSH
                     DLEGVEPTAHAMPVLDVIRADETRPGFSQDEALANAPRRVMGQFQIPKVIE"
     gene            24821..>25270
                     /locus_tag="PLP58_08005"
     CDS             24821..>25270
                     /locus_tag="PLP58_08005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009961611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HRJ08494.1"
                     /translation="MSLASQTLAALRRQIAAKEIHPRDIVTDLAAAIEARNGELGAYL
                     SWDADKALAEAEQVATSLPLGGIPIGIKDNINVTGQPCTCGSRMLAENYTAPYDAGVI
                     TKLRAGGAVPFGRMNMDEFAMGSSTENSALGLTRNPRDPRRVPGGSSG"
BASE COUNT         4646 a         7873 c         7897 g         4854 t
ORIGIN      
        1 cgcgcggccg gaggcggagc tggcgcggcg cgtgcgcgag gtggcggggg caaaagcggt
       61 gatcgcgggc accttcgatc cgcatggcaa cgaggacgcg gagttcctca agcacgcgga
      121 cctggccttc gcctacaagt actatccgca ctacgacgcg catctccagg gcgagcgcgc
      181 cgcgcgcatg atgatccgcg ccatccgcgg cgactaccgc ccggcgcgcg cggtgcgcac
      241 ggtgcccatc ctcagcgcca cagtgtatca gtggaccggc cagcatccgt ggagcacgct
      301 ggtgcagcgc tgcctgacct gggaggcgcg cgagccggac gtgtacgtca atttcttcta
      361 cggcttcccg tgggcggatg tgccggacgc tggcatgtgc ttccaggtca tcacgaatgg
      421 cgacgccgcg ctggcaaaga cggtggccga cgacctcgcg gacacggcct ggcgaatgcg
      481 cgcggagatg ttttccgcca cgcccattca tcccgtggcg gaggcggtgg ggcaggtgaa
      541 aaaagcgctc gaacaaggcc gcgcgcccgc cgtgctcgcc gactacagcg accgctccgg
      601 caacgccacc tgggtgctgc gcgaggtcat cgcgcaagac ctcgccaaag tcgtgatcgc
      661 caccatcaag gacgaccgcg ccttgaatgc gctggcccgg gcgggcgcga agcagggcga
      721 cgccttcgac atggatgtgg gcggttatgt ggacgagtcc gccggccagc cggtgcgcgt
      781 gaagggagtc atcgagcact tgagcagcga cggcaaaggc ggcgtcaaag cggcgcgcat
      841 ccgcttcggc cgcgggaacc tgctcatgct cagcccgcag ctcgtgcaat acatggaccc
      901 gcagtccatc cgcaaagacg gcgtggacat cacccagttc gaggtcttcg ccatcaagtc
      961 ccgcgtccac ttccggcgcg gctttgatga caacggcttc gccaaaacca tcctcctggt
     1021 ggagccgccc ggccccttca tgggcaccgt gcatctcgac gcgctgcgct acgagaacct
     1081 gcgtgtgcgc gagttttatc ccttcagcga gaagtgacgc aagaagcccc gccacgcgcc
     1141 cgacatggag ggatgcgttt gttttttctc ctcctcctgc tcgcctccgc ccaagtccgc
     1201 gcgcaggagg cgccgccttt gcaggagtgg atccatgagg cagtcaaagc cggcggcggt
     1261 gtggtgacgg tgccggacgg ggagcatgtc ttcaccgaga cgctggtcat tgaggacgcg
     1321 aaaaaactcg cgctgcgcgg catggggcgg gagggctgcg tgctgcggat gaaggacgcg
     1381 gccaagccgg tgatcgagat tcgcggcgag gtggagaggg tggaaatcgc gggcatcatc
     1441 ttccagggcg gcgcaggcgt gcgcgtgcgc ggcggcaagg acgtccaggt gcgcgactgc
     1501 atcttcgaag ggccgggcgt ggtgatgctc ggcgcgcgcg agtccggcgt ggagcgctgc
     1561 agcttccgcg atggcaaagg cgccgcggtg gtgctggacg agtccgcgcg gcagatcgtg
     1621 atccgtggca atcaagtcac gcgcatggcc ctggccttcg acctgcgcgc cgccgcggag
     1681 tgcgtgctcg aaggcaacga ggtgcgcgac tgcgccgcag ccatccgcat cgcgggtctg
     1741 gagccggcgg gggcgcaaaa gcatgtagtg cgcgacaacg gattcttcga catcggcggc
     1801 gacgcgctga tcgcggcggc agggacgcgg gagattctca tcgagggcaa cgaggtggat
     1861 caaacgcagg gcttcggctt tgtcatcgcc ggcgaagggc atgtgctgaa ggcgaacacg
     1921 ctcaccggca cccaggccgg gcagatgcgg gacgagcgcg cggacaagcc gtgaaagttg
     1981 cgcctgccgc cgggccgttc catttctccc gatgatgatg aaacccgtcc tgctcctgct
     2041 cttcctcgcc agcctcgcgc ccgccgccga tcccacgccg aggccgaacc ccgcgcgctt
     2101 cgccaaagac atcgccaaat tcgccgccga gcgtcccgag aaaggcggca tcgtcttcac
     2161 cggcagctcc agcatccgcc tgtggacgga cctgaagcag gacttccccg acctgcccgt
     2221 gctcaaccgc ggcttcggcg gcagcgtggc gaacgacctc agcgtttatt tcgagaccgt
     2281 catcgcccgg catgagccga ggctgctcgt cacctacacc ggcagcaacg acctcaatgc
     2341 caagctcacc ccgcaggagg cgctggcgga ttacaccgcc ttcctcgaca aattccacgc
     2401 ccgcttcccc gccgcgcgcg tcattctcac ctccgtgaag atcggcgaga aacgcctggc
     2461 ccagatcccc caggtgcatg agctgaaccg catgctccaa gcctggaccg ccgacaagga
     2521 atgggcgcgc tacctggact gcgacagcta cctggccgat gacaaaggcc accccatccg
     2581 ccaatactac cgcgacgacc tgctccacct cagccctgaa ggctacgccg agtggaaaaa
     2641 aatcctcgag cccgtcctgc gcgaggaatg ggccaaggtg cgctgaagct tcggcacggg
     2701 caggtgggcg cattcgccag aatgcgggtg ctgtgcctgc gagcgggagc acatgccgcg
     2761 ctttggcaag cgcgcctaca ggcagcgtag gcgcattcgc cagaatgcgg gtgctgtgcc
     2821 tgcgagcggg agcacatgcc gcgctttggc aagcgcgcct acaggcagcc ggcctgatga
     2881 aaggccgccg cgcttgatga aaaaaacatc acggttgatc gaaaaatcat caacgttgaa
     2941 ggaaaaacca ccgcgcttga tggattccca atcacccgcg gctgaaaatc catcgcgcgc
     3001 gcatgaaaag gcatcaccca cggctgattt ttcatcgcgg gcgattgaaa atccgccaag
     3061 cgttatgaaa attcaatcaa ctgcggtcaa aaatcagtca cttttgactg attgaggccg
     3121 gtgggggggc ttcggaaggg ggggaggggg gcttccgaag ggggtatttc acgtttcgag
     3181 cgtgaaatac cccgttttca gctccctgcc ctcaaacctg cggcgcgggc gcatattcct
     3241 ccacgggggg agcctccggc gctgccgcag aacgctcaaa ccacggggtc agcagcgcct
     3301 gcagctcctg cgcgtcggga atgttgcgcc gcgccgcatc cgccttccca gccctttcga
     3361 tccccgagtc agcaccagag atgaaaaatc cagtaatcga aaaaaaacct tcacaaccca
     3421 tctgtagcgg acgaatcttc ccgttcatcc acagcaaccc atctccgaat cccgctcgcc
     3481 gattcctttg tgtgtctata caacgtccgt ccaataaaca gagccccatg tcatgcgaac
     3541 gacagccatc ttgatactga tctgcgggtc ggttctgcta gcacaggaca gggaaattga
     3601 tccttttgca gatgcttcga atagcacgtt gccagccaag gcatctactc cgccgaagac
     3661 tgaggatttg cgaccttcag cagcggatct gaagttgctg ggagagcctt cacttgaggg
     3721 gcgcacagat gctgatcgtt tccccacgat cagggtgatt tggtgccgga catttcatcc
     3781 acccgtttcc ctcagagcct acctgacgaa ggatggaccg cgattgaggg tcgctcgcat
     3841 gtctggcaag ggcggctacg actgggggac tctcgacttc gagaacgatt ttccactcag
     3901 ggctgagaca tggatgcgcc tccttaatct actgaatgcc gatggtgccc gtcagccgtt
     3961 gaagaacgtc aaacaagagg acatggatta tctgactgga ttggatggct ctacatggaa
     4021 gctcgaagtc tctgatcagg cgggttacac tgttgacgag gtttggacac ccatgaacgt
     4081 caccaatggc gaccctgaca taaagaagct ggctgaggat caaggcatac gcctcgacaa
     4141 ctttgtgaag ctctgcagtc ttctcatcca gtatgcgccc attgatcccg agtcaatcta
     4201 ctgacactaa cgggtcgaac agcccgaagt gtctttgcct ctatagcccc gacgttcgcc
     4261 acaagattca tgaacgccat caaccagttg attgaaatcg gaggtgcggt gttctttctg
     4321 ttgtttggcc tttgggccac cgcctacgca tatgggttcg tcggcgagaa gctggccggg
     4381 agtttttgtt ggaaaccaga attcaagcac gcgcttcgtt ggctgggacc gctgctcgtt
     4441 ttgctttgtt gcgcgtcact cgtattcctg ttcacgcagc gttaagcaga tgaaggatcg
     4501 attggcgaac aaacttgtct cccgcagact tcatccgact cctaccatga gcagcctcag
     4561 cctcagattg cccgattcga tgcaacgcca cctcaagagg gcggcggatg cggatggcat
     4621 ttccatcaat cagttcatca gcctggccgt ggccgagaag ctctcggctt tgcagaccta
     4681 cgacatcatc gccgagaggg ccaaaggcgc gtcccgggag agtttccttc aggtgatgtc
     4741 gaagccccgc ccccgttcgg tgtatgagga ctcgacgccg ggctaaaatc gtccgctggc
     4801 attgtctaga aattccacta ttctacggcc atgacacaag tcacagcact gctcaaggaa
     4861 gcatcgaagc tggatctccc agatcgggcc gaacttgtaa cctcgttact tgaagattta
     4921 gaccctgaac cgcatgatgt aagcgatgag gaggtcttga agcgcctgga agaactgaag
     4981 tccggcaagg tcaagggcat ctccaaggag gaattttgga aggcatgcgg acgcccataa
     5041 gagatgcgcg ttatcagaca tccaaagctg gcagacgacg ttcgcgatgc cgctttgcat
     5101 tacgcggaaa tttcagagag agtctgctct tcgttctgga atgagcttga agcggttctt
     5161 gaatccatcg agcggaaccc aaggagccat cattacgatg cttgtggatt aaggagggct
     5221 aacatgaaga ggtttccata tcatctgttg tatgatgtcg aagatgatac tgtctttatg
     5281 gtggtgttca gacatgacag gagacaccca agtttcggca ccaggcgaaa aatctaacac
     5341 cagggcttga cgttgggtct cacctccgcg tggaggttgc gcgggagtgg gcgagggcgg
     5401 aatgatgtcc gtcgtgcaag caggacgggc gaaccacctt cacgcctcga tgaaggagca
     5461 gatgtattcg cagatgccgg aggtgacggt gatgtcgaag ccggacttgg cgggcacctc
     5521 gaaatgggcg ccggcctcat aagcggcggt ggattcgccg ccgtcgagct tcacagtgca
     5581 ggcaccggcg acgatctcca tgcgctcggc tttgtcggtg ccgaagtggt aggcgccggg
     5641 gtaaatgagg ccgagggttt ttttgccgcc gccggcaaag agcagggtgt ggctgacgac
     5701 gcggccgtcg aaatagacgt tggccttggt gacggcggtg acgccgggga attcggtggg
     5761 gatggcggac atgggcggcg agtttggcac gcgctgtgag gcggggcaag cgtcccgcgc
     5821 gtctgcaacg agatgggtca cggcttttcg tttggcgtgc aagtcgttgg agttcaagct
     5881 ttagcttgtt cactgacctc ccgcggcgga gccctctccc gtgtcgccta aaggcttcac
     5941 tccaatatgg gaatccaaca ttccacaatc gccttcaaga gaccgtgcga ggagggctgc
     6001 gctcttattc cgcctccgcc agcctctggc gatagacgcg gcgcgcgtgc tcgtaggcgg
     6061 cgatggcgtc cggcatctcg gcctcggcat aggtgtcctt tttcgattcc cagcagcggt
     6121 ccacgctgtc gaggcaccat tgcaggctgc gtttgccggc gcggacgggt ttgccgccga
     6181 cgaggatgaa gacggggttg gtgtgggagc tgggatggat gcgcagggcc acccaactgc
     6241 ttttttcaat gggcacctcg aagctgatct cgcgctcgct gccatcggcg gggatgacct
     6301 tttttgccac ggggtagcca ttgacgatga cctcgacggg cacggtgcgg gtgtcgccga
     6361 tgcgcgcgcg ctcgaggtgc cagtagggct tgcggtaaaa ggcgtcgtat cggcggccca
     6421 tggggagatc ggcggtgttg ggcggggctt tctggccgcg cagggtttca tccggctgct
     6481 ctgggagaag cgcggcgaca tcggcgcgca cggtgacggt ggcgggggcg tcgaggcgca
     6541 gttcgctgcc tttttcgccc aagggcaggt cattgacgcg catgtccatg aggtggctgc
     6601 ggccgtcgcc cacatagcag cggccggcct cgatgccggc gcaccaggca tcataggtga
     6661 ggcggccgtc cactttgaca tagctgcggc ccatgccgac gcgctcgccg tagatgcagg
     6721 ggaagtcggt ctcgccgctg atgcgggtgc ggaagccggc gttgagggtg tgataccaga
     6781 tgttcagctc ccagtgcggc gcggtgtcgg cggtggacat gaagtccacg gcgggcacgg
     6841 gtttgccgtc ggggccttcg acctcgtggg tgacggccat gatgtattcg ttggcgccga
     6901 tgccgtcgaa gggcgggatg aggtagtttg gcaattcatc ggtgggcact tgcaggccgg
     6961 tggcggagtg cgcggggccg cagactgcgc cctgcttttg cgcccagcgc agggtgttca
     7021 ggccgagcgt gggccagtgg tccatggagt cgccgccggg atacatctgc tcgcgcagcc
     7081 gcagcaggca gaggtggccg ctgcggtggg agccgaagcc gctgacctcg atgtcgtagc
     7141 gcaacaaaaa gggatagcgc gagactttgt cctcggcgcc ggtgaagaat tgcttctggt
     7201 agtcgaagca ggggccccag gtgaggttgg cgccgatttt caaatcctcg ccctggcagt
     7261 ggcgggccat gtccggcgcg tggacgccct cggtggggga ggcatagtgc tggcagccgg
     7321 cggcgtggat gtggtggtcg ccgctccacc agccgcgcgc ggcggggtca atccagcgct
     7381 tgacctggaa gctccattcc ttcgtggcgg cgtccactgt cacggggcgt gtctcgggca
     7441 gggattcggg gccgcgctgg aagacgatct cgtattcgcc atcgggaagg cgcacgcttt
     7501 cgccatcacc gcgatagact tgctgctgga agccgaagtc gggcgcgaga cgcttgatct
     7561 gcgagggatg gacacggccc gcgcggtcgc ggatgaggag ggaggcgatg cagggtttgc
     7621 cggcttcgtc cgtcacccgc agtgtgatct catgcgcctg gcggcaggaa aacagaatgc
     7681 tggtctcgcc gcggaagccg aggtcctggg tgccctggcc ggtgtcgaag gtgaaggtgg
     7741 cctcgcgttt tcctgtgtcg cggctgtaga tctggatgat gcggtattcg agttcgagac
     7801 cgctgagggt ggggcgcaat ggcggcgcgt cgaacatttg cacatcggcc caacgcgcgg
     7861 agtagggggg cgcggcttcg gggatgggat cgttcgggcg cttgggccgc tcatgcacgg
     7921 ggccggcctg cgggctgctc acgcgcagcg gcgcggtggt gccggcttcg ttgaccacgc
     7981 gcacgaggaa ctgccgccag ccggcttcgt cgagctcggg gctggccgcg ccgggttgca
     8041 ctttcacgcg ctgctcggga ttgatgtgga tgtggaagag cgcgtgcgag tcgagtaatg
     8101 cgcgcgcctt ttccacggcg tcggcgtcat tggcctgaat acaggcgcgc agcgtgtgca
     8161 gctcgtccga gttgaaggga gtgccgagga actccatggc ctcgatgaga cgcccggtgg
     8221 aggcgaggaa gggctgcggt tcgggatcgc gggcatgaag actggcaacc agaacgagac
     8281 atgaccccca aagtgttttc atgcgtttca aacgtttgtt gagggcactt atttgcctga
     8341 ggggctgttc tgctttggga ttcaagaagt ccttttttcc cttcttgcca tttccgtcgg
     8401 gcatccctag tctccgcggc catgactgaa aaccaaatga aagtggactg ggcggcgctg
     8461 gaggcgaagc cggagttccg cgccctgctc gcgcgcaagg cgcgttttat caccgccgcg
     8521 tccgtgtttt tcgtggtgta ttatttttcg ctgctggtgc tggtgggctg gtttcctgat
     8581 ttgatgaaga aggaagttct tgggcggatc aatctggctt atctctttgc tctttcgcag
     8641 ttcttcatgg cctggggcct ggcctggctc tacacgcgca aggccgcgca atgggatttg
     8701 gaggctgcga acatcgtccg cggccattga ttgcgtctct tttccacacg tcactttccc
     8761 aattttttct catgaccatc gcagtctgga tgttcctcgc ttttgtcgta gccaccctgg
     8821 gcattactgt ctgggcggcg aaaaaaaaca ccagcgcggg ggcctacttt gccgccgggc
     8881 gcagcatcac cggctggcag aacggcctcg ctgtggcggg tgactatatg agcgcggcca
     8941 gctttctcgg catttccgga atgatcgcct tttacggtta cgacggcttc atgtactccg
     9001 tcggctggct ggtggcctac cttaccgtgc tgctcgtggt ggccgagccg ctgcgcaacg
     9061 cgggcaagta caccatggct gatttgctcg cctaccgtct gactccgcgt cccgtccgcg
     9121 ccatggcctc gctgagcacg atcaccgtgt ccacttttta catggtggct cagatgatcg
     9181 gcgccggagc gctggtgaag ctgctgctca aggacctgcc ctggatcacc acggaatggg
     9241 ccatcatcgg tgtgggcgtg ctcatggtgg tgtatgtggt cttcggcggc atgctggcca
     9301 ccacctgggt gcagatcatc aaagccatcc tgctgatgag cggcaccatc ctgctgagcg
     9361 tgctggtcat gaagcatttc ggctacagct tcacggcttt cttcgaagcc atcgccaacc
     9421 tcacttacaa ggacgcttcc ggcgcggaag tgacgcgaaa tttcctggaa ccgggaatga
     9481 agtttggcgc ggcggtgacc aaaggctggg ggccgctgga tcaaatttcc ctcggtctcg
     9541 cgctcgtctt cggcaccgcc ggcctccccc acattctggt gcgcttttac accgtgccgg
     9601 atgcgaaaac agcccgcgtc tccgtggtgt gggccatgat catcatcggc gcgttttaca
     9661 tcctgaccac cttcctcgga tttggcgccg ccaccattct caagccggac tccatcccga
     9721 cggacaacat ggcagctccc cagctttcgg aagcgctggg cggtccggtt ttcttcgcct
     9781 tcatcagcgc ggtggccttt gccaccattc ttgcggtggt ggctggtctg accatcagcg
     9841 ccagcgccag cttcgcgcac gatttttaca ccaacgtcct ccatcacggc aaagagcatc
     9901 gtccgggtga ggaagtgcgc gtggcgcgca tcaccgcgtt tgtggtcgga gccgtctcca
     9961 tatttctcgc catcaagctg caaaacgtca acgtggcctt cctcgtcgga ctggctttcg
    10021 cggtggctgc cagcgcgaat cttcctgtga tcgtgttgag catcttttgg aaaaaattca
    10081 gcaccgcggg tgccgtgctc ggtttggcgg tggggctggt cagcagcatt gttctgattg
    10141 ttttgagtcc cagcatcatg ggagtggatg gccctgaggt ggcagccgcc aaacgccatc
    10201 tcatccaggc tgacgcgatc tttcccctga ccaatccggg cattgtcagc atcccgctcg
    10261 ggttcctggc ggccatcctt ggttccttgt ggagacgcga gcccgaagcc gaggcgaagt
    10321 tctgcgaact caaagtgcgc gcgcacacgg gtcttggcgc cgagaaggcg gtgagtcatt
    10381 gaccaaaaaa agtcggggcg gaccacccgg tccgccccga cttttttgaa ggttcccaac
    10441 agccatatcg gttaacccac accatttgat gaaatggttg atcttgcgat ctgatgggag
    10501 tctgccacag gccggaaagg atgcgtcagc ctgctgtcat cactttgtaa gaggtttgtc
    10561 aggcttggcg ggctttccct cgcggcgcag aaacggggcg aaacgggatt cgaattggcg
    10621 cggcaccacg cattggatgt ccgccacgcc gcgctcgtag tcttcggcaa ggaccttgcc
    10681 ctgctcatgc gcgagagcca ccagatccat gcgctccagc gggatgctca attccaggcg
    10741 caccacgcgg tctaacaaca tgtcatgcag acgatgaaag aggtcatcga gcccgcttcc
    10801 cgtcctcgtc gaaatgaaaa ccgcctcggg aaagcggcgc cgcagttcca tctggcgcgc
    10861 gtcatccatg aggtcggttt tgttcagcgc cagcagcacg cgcttttccc ccgcgccgag
    10921 ctcggccagc acctgcgtcg tggtctggta gtgctcgaga gcgcggggtg aagaggcgtc
    10981 caccacatgg atgaggaagt cggcgagcac tgcctcttcg agtgtggcgc ggaagctttg
    11041 caccagatcg tgcggcagat tgcggatgaa gccgacggtg tccgtgagca gcagaggttg
    11101 tccgtccggc agctcaagac ggcgcgtcgt ggtgtcgagc gtggcgaaga gcttgtcctc
    11161 ggccagcact tcggcttcgg tgagtttgtt gaggagggag gacttgcccg cattggtgta
    11221 acccacgatg gcggcatgtg gcacgggcac gcggctgcgc tccttgcgca tggtgtcgcg
    11281 ctgctttttc acctcctcca gatccgcctt cactttgtcg agccgcttgt aggccaggcg
    11341 gcggtccact tcgagctgcg tctccccttc tccgcgcgcc gccccgccac ctgctcctcc
    11401 tccgctgccg ccctggcggt ccagatggcc ccacatgcgg gtcagtcttg gaatcgagta
    11461 ttccaaacgc gccagctcca cctggaggcg cgcttcgcgg gtttgagcgc gcatgttgaa
    11521 gatgtccagg atgacttcat gccggtcaat gaccgcgatg ccagactcct gttcccatgc
    11581 gcgctgctgg gcggggctca gctcgttgtc gaagatgatg cagtcacatt ccagctcctt
    11641 cgccttcgcc atcagttcct gcgccttccc tttgccgatc aaatgccgcg ccgtgtgctc
    11701 gcgcgcgaac acaaggtccg ctgacacgat tcctatgcca agggtgcgga ccagctcctg
    11761 cagctcttca agcagttcag ccgccccggt ggaatggcgg cggtcaaaat acgctcccac
    11821 gagaagcgcg ctccgcacca cctggggttt ctcacgaatg tcgaacatac gccaacatga
    11881 cgcgcgcgcc ccggcttgcc aagcgccgca gttgccgtca tgcgctttcc atgccaagtc
    11941 agcggatgac acgccgcctt gccatattga gctttttgtc agccctcgcc tcctgcgggc
    12001 ccgtggaccg cgatggcgcg cggctctcga cctatgaaaa cgacgccacc gaggcgcttg
    12061 tgcgcgagat gctgcgcacc ctgcccgacc tcaatccagg cgtgcccaaa agccactcca
    12121 tcagtctcgg cgagattgtg cccaagcgcg atttcactcc tgccagcgtg ccctttttca
    12181 aacgcttcga agacaccgga ctgcgcatcg tcagcgccgc cgtgctcacc accacgccgc
    12241 cagacaacat catcattgac cccgaactgc gtgtggctgc ctacatcatc caaatccgcc
    12301 acatgaaaca gaccatgccc ggccgctggg aatacgaagc gggctggtcc tacaaaaagc
    12361 agttccaacg gaaaaaatgg acggtcactg ccgcgaatgg aagctacaaa gtcgaggccg
    12421 gcgaaattct cgatggcaac ctggagtagc taaaaagtga ctggctgcgc tggcttcgcg
    12481 gtgatttcgc agtccacttc tgaagaaggg tggtggctct catgacattt tgcgcgccca
    12541 tcgggcgagc agcgccttcg gattccaggg gtgaaagccc tccagactga ccagcggcct
    12601 cgattcccgg caccactctg ctttgtaggg ctggtctcct ggcagcatgt cgaagatccg
    12661 catttggcgg cggctgcaca gttccatgct ccagttcagc acaagattcc ccggggaaag
    12721 ccgcgccaga gccggatccc agccgtgctg ataaaatgaa aactcgtctc cttccgcgaa
    12781 tccgtacaaa gccgcaacca tccctcgttc gggtgtggca agactgggca tcagtgcccg
    12841 gcctgatttc agccagcgcg cgccaagctc tctgtgaaaa ccccacgcgc cgtccgtcac
    12901 gaaatgactc ctccccttcg gccagtggaa agcgtgcagc cgggccagct catccattgc
    12961 cccgcctccg ccttcttcgc agcgccggcc ttcatgcttt tgcaggaaga taatgttttt
    13021 tctgcgcatt ctcctgcgcc agcgtccctg actgcccggt tcgatttcct cccagttatt
    13081 cggcagtttt aaccagcggc tttccagctt ctccgccacg cccgctccag cacagacgga
    13141 tttcaccgcc ttcatcagcc agtccagatt cggtgattcg gcgggaatca ttggcagcca
    13201 aaccccgtgg caggcatggc cgggcagatg cgtgagcgcg tccgaaagca gcggcccaag
    13261 ctccgcctcc ctgcccagtg gcaccagagc atccatcctc tctcccagca ccgggccctg
    13321 tccgctgatc caggtcagca gtcgcaaatg cctccgcaat ccatgatctc ccttggacag
    13381 catgaatgga gcaatcgcct cgacctcgcc ccggtgcccc cgaaccacga tcacggccag
    13441 cttcctgcct tctccgaaat gtctccacca ggtccaaatc cagtcccagc gcagaaaggg
    13501 cgaacgagtc gcggacttct ccagcagctt gtcccagaat ggttcaagtc tggccaagcc
    13561 ctcctcttcc tcaatcacct ccgtcacatg ccggctcgtt tcagtccggg ctcgcgggca
    13621 tggcacaaaa cctccccctg ccttgaacag ttggctgaga gtgccggagg atgacaggct
    13681 ggcttccacg aaaacgggcg tcaacgagac gcccgtcaat tatcgtattt atagccggag
    13741 cttcaagagc attctcaaaa ctatgtcgcc cggaaacgcc ctctccatca atcgttgaag
    13801 aaatgccggc ccgtctcgcc agcggtcgtt ttgttgtcca cttcccaata cacatcttcg
    13861 aaaatggatt cgggttcagg atagggacta gatttggcaa actcggcaga ttcgtcggct
    13921 tcggcttgag cttcgcggtc aatttttttg agttgatctt ctgtagccac accctcggac
    13981 agcaagaccg ctttccaaat ctggatcgga tcatgctcgc gctggtagcg ctccacctct
    14041 tctttggtgc ggtacccatc cttgtcttcc gttttctgat ggcgcgcatc cgccaccgag
    14101 tgcccgaggt agcggtaggt gtcaatttcc agcaaagtcg gcttcgactc gttatgcgcg
    14161 cgctcgattg ccttctgcac gccggcacga acatcataga cgttctcacc cacaaacttg
    14221 tcccacgcca tgccgtaacc ctccgcgcgt ttcgccaggc agccatcgaa ggctgaggaa
    14281 cgcgcctggc tcgtgcccat cgagtagcca ttgttctcga tcacataaat cacgggcaga
    14341 tcccaaagcg ccgccagatt cagcgactcg tgaaacgcgc cctgattcac cgcgccatct
    14401 cccaggaagc aaagcgccgc gcctttcagc ccgcgatact tcagtccata ggccagaccg
    14461 agacccagcg gcgtctgccc ggccacaatg ccatgcccgc cccagtaatt cttgtccggc
    14521 gcgaagtaat gcatcgagcc gcccttgccg cgcgagcagc cagtggcctt gccgaaaagc
    14581 tccgccatgc actcattcat gcccatgccc accgccagag cgtgtccgtg gtcgcggtag
    14641 gcggtgatga cgtgatcgtt ctcgcccatc agcgacacgc agcccaccgc caccgattcc
    14701 tggccgatgt aaaggtggag gaatccgccc atgcgacccg cttgataatg ctggagggaa
    14761 acttgctcga accggcggat gcgggccatt ttccgataca gctcgatctt gtcatcggcg
    14821 gtgagtttgg cgttgatcgg ggcttcagag gaggaggcag gggtcgcgtt cttggtggcc
    14881 atgatgtgag tccggggata ctagcgggat gaatggcgcg tgcaagtcac agtccacccc
    14941 ccgaaaatct tccctgttat gttccacatg gaacaaaact ccccccttcc ctgacatcca
    15001 tttcctttga tctttgccct ccctccccca tcttaaccac cccatgtccg ccagcctcta
    15061 caccttcccc aagcactacg atgtcatcgt ttgcggggcc ggccatgccg gagtggaggc
    15121 cgccatggcc gccgcgcggc tgggttgcca gacggccatc ctcacccaaa atctggacac
    15181 catctcccag atgtcgtgca accccgccat cggcggactc gccaaaggcc acgtcgtccg
    15241 cgaaattgac gccctcggcg gtgtcatggc gcgcaatacc gacgccaccg gcatccagtt
    15301 ccgcatgttg aacgcccgca aaggcccctc cgtccaggcg ccgcgcgcgc agtgtgacaa
    15361 gaaagcctac cagttccgca tgaagtggct gatcgaaaac cagcccaacc tggatctcca
    15421 ccagggcaac gccgccgaga ttctcgtcga aaacgacacc gtcaccggca tccgcaccag
    15481 cctgggcatg atttaccgcg ccaaggccgt catcatttcc tccggcacct tcatgcgcgg
    15541 gctgctccac gtcggattgc aaaaccaggc cggcggacgc atgggcgaca gcatctccac
    15601 cctctccgac tccctgcgtg aactcggctt cgaggtgaag cgcttcaaaa ccggcacccc
    15661 ctgccgcatc aacagccgca gcattgactt ccgccaatgc gaacctcaac ccggtgactc
    15721 acccgcccct cccttctctt acctctcagg agtcgtgccg gaagacgagg agaataccga
    15781 aattcgaaat tcgaaattcg aaatccaacc ttccttccac cacttcaccc tcaactcctg
    15841 gctccccgaa aagttccacg tggaacaaat tccctgctgg atcacctaca cgacccctca
    15901 aacgcacgac atcatccgcg ccaatctcga caaatccccc atgtattgcg ggcgcattga
    15961 gggcgtcggg ccgcgctact gcccctccat tgaggacaaa gtcgtgcgct ttgctgagaa
    16021 ggatcgccac cagatcttcc tcgaacccga gggccgccac acccgcgagt tctacatcaa
    16081 cggcgtctcc acctccctgc cctacgaggt gcagctcgac ttcatccgct ccatccccgg
    16141 ccttgaacgc gccgagatca tccgccccgg ctacgccgtc gagtacgatt actgcccgcc
    16201 cacccagctc ctgcccaccc tcgagaccaa gcgcgtctcc ggcctctact tcgccgggca
    16261 gatcaatggc acctccggct acgaggaggc cgccggccag ggtctgctgg ccggggccaa
    16321 tgccgccctc aaagtccagg gccgcccacc cttcatcctc ccgcgcagcg ccgcctacct
    16381 cggcgtgctt gtggacgacc tcgtcaccaa aggcgtcacc gagccctacc gcctcttcac
    16441 ctcccgcgcc gaataccgcc tcctgctccg gcaggacaac tgcgacctcc gcctcacccc
    16501 gctcgctgcg cagatcgggc tcgcctgccc cacccgcacc cggctcaccc aggaaaaagc
    16561 cgcccgcctc gccgaggcca tgaccttcct ccagaccacc acccacgacg gcctgcggct
    16621 ggagaaatgg ctgcgccgca ccggcaacga ccacaccacc ctgcctgccg agctccgcgc
    16681 cgccttcccc gacgccatct ggcagctcgt ggaaaacgac gtcaaatacg agggctacat
    16741 ccagcgccag gagatgatgc tcgagcgcac cgcccgcatg gaggaaaaag aaatccccgc
    16801 ccacttcgac tacgccgccg tccacggcct caaactcgaa gcccgccacc gcctcagcca
    16861 gatccgcccc gccaccctcg gccaggccgc ccgcatccag ggcgtcaccc cggcggacat
    16921 cgccctgctc gccatcatgc tgcgccgggg gtgaggttca tcggaaactc caggtggacg
    16981 ccattccgat tttggttaga atcgcggcat gattctgtcc tttgcggatc gtgaagctga
    17041 aaaaatcttt cgtgggcaag tcagccgcaa gctgccgcgt gagattcaag acagcgcgcg
    17101 cgaaaagctc atgctcctcg atgccatggt gcgcattgaa gatctgtggg ctttcccctc
    17161 cctccgcatt gaaaagctcc agggcaaacg cgccgggcaa tggagcatcc gtatcaacaa
    17221 acaatggcgc atctgctttt tttgggacgg cgaaaaagtc accgtcacag gcgtcgaaat
    17281 caccgactat cattgaacca tgaaaaccac ccccgcgaaa gaatccccga cacgtctggc
    17341 cagcgtgcat cccggccgca ttctgaaaaa ggagatcctg gaggcacggg cgctgagcca
    17401 gacacaactc gccgcagcca ccggactgcc ggtcagccgc atcaatgacc tcgtcaaaga
    17461 gcgccggggc atcaccgtgg acagcgccat ccgcctgagc aaggccctgg gcacctcggt
    17521 ggacttgtgg ctgaacctcc agcgcgcctt cgaccttgaa gaagcccaga ggctcaaggg
    17581 tgaggaatat cagcgcatca cccccctggc tgcttgatct tgcaccagcc atgcaccccg
    17641 ctgccacact gctgactctg gtcatttgcc tgtccatgtc cttcgcttcc cgcgcgaagg
    17701 acccgatgcc ggtcgcgccg tttctggcca gccacatcac ctctctggaa atccgccagc
    17761 ttcccttccc cgaggcggtg gaggcgttgc gacaggcggt ggccacggag gagtgggtgg
    17821 gcgcggaggt ccagatcgcc cgctaccggg agccgatgca gtccgaagcg catccctggc
    17881 gtgatgagta tgaaaatctg gtctctgtgc atttcaggga catctctgtg tatgatgctc
    17941 tccaggccct gtgcgcggcc tgcggattcg agatggatgt ggaagtgttc aagttgcggt
    18001 gtttcgagac atccggccag gtgggcaggc ctgcctttga aaagctcttc cccgaggccg
    18061 cccaacgcgc ggatcaactg agaaaattga agattcccgc ctttcaaggc gatggccttt
    18121 cagcctatga agccatgaag aagctccagg acgaagtgtg caagctcacg ggtcggcagc
    18181 cgccgccctt cgtgtttatc agccagcggg atcggaagag tcccggcaaa atcggagatg
    18241 acacgcatca cccgattcat ttgaatttga aagacacccc aaccccgtac ttcgagatct
    18301 gccgttacat cgccgaactg tccgcgcata gcttccgcat gagcgctgac gggcatgtga
    18361 tcctgcgcag ggtggcggac atctatgatc ctgtgcgttt ccgagttctt ccggtggaat
    18421 tgttcgcccg tcagttgggc aagcctgtgg cggaagtcac ggaggaggcg atgcaggcat
    18481 ggatggagga agccggatgg agtgtgtatt tcctctccta ccaccgcccc tccggccgcg
    18541 ttttcatcga gggcttgtgg gcgggtgatg agggggcgct gttggaggca ctggaaaaac
    18601 ggccttgagc agggcattcg ggtttgggaa atgccgggga tgacagcggg agctgcgcgg
    18661 aaagtgggtg cgacggagga ggctgcgatt cgcttgcttg ctttttggaa agttgtgaat
    18721 caatcgcggt ctataagttt gcattttgca aaaatataaa tcgctcttga attatatctt
    18781 attattattt gaagtcgaga agctcaatag attcatttat ttttattatt caaacacatg
    18841 tttcattagt aattcctcta ttatcagaat aaaatctttg tttttgttaa agattcatgc
    18901 atttgatttt ttgctaatgg ttgaactatt tgtggaacaa gccttgcttt ttgacaaatg
    18961 tgcgtttctt cgctctggcg ggcttttctg aaacttgtcg ccccggtttt ggcgtactga
    19021 cgggggtggg gatttgggga ggggctgccg gtgcgctttt tctgatcatg agaatcctgg
    19081 cccgatattt tgacaatcct ttcgatgatc ccggaatcag cctggcggag ctgctggctt
    19141 tttcgacgga tcatctgggg cgactgcgcg ccaggaacgc tcagggggag ttcgcggaac
    19201 cggtggcggc gctggagtcg gcgctggcgg ggctgaatga ggcgagcatg caggacttct
    19261 cgaatctggg cctgcgcaag gcgcgcaagc gggcgaagcg ggacttccgc cgcaagctac
    19321 tgccgggggc gcttgagaag gtggatgtgg cggtgctggc gtttttggag gggggccgga
    19381 atgtgcggca gagggcgttc ccgcaggggc ggacgatttt ccgcacttgc gcggacgatc
    19441 atctgcgcgc gcatctgcgg gtgatggatg aggtggtgca ggagcacgcg gcggcgctgc
    19501 cgccggcgat cgtggagttg tccgccgggt tgctggcgca gtggcaggag atttactcgg
    19561 gcagcgagtc gagcacgggc gcgaaggctg cggcggaggt ggcgcagcgc caggcgcgcg
    19621 cggtgctgca aggggtgctc tttgacaatt tgctgctgct ggcgcgccgg cacgcgcggg
    19681 aaccggaccg cctggccgac tacatgcagg agtccctgct gcgcgaccgc gtgcgccggg
    19741 tgcggaaggg ggcggcggct gagccgtaac aatgcagttg aaccacgaat ggacacgaat
    19801 ccatgcggag aagagacgga tgggtggtca tcacccaagg ggtgatctcc cggcgcttca
    19861 gctcaccgga catgatgcct ccaaaattcg tgctcattcg tgtccattcg tggttttcca
    19921 tcgcatgaac acggatgaga caggaatgcg aggtcctgcc tgggcagggc gggctgtccc
    19981 cagcccgccg cgcgcggcgg tgcggctgct catttgcaat ggcggcgcgg cagggttgac
    20041 accgcgcggg gccgtccctt cattgcgcgc catgtccgac ccccagccaa taacgcccgc
    20101 agtctccgtg gagaacctga cgaaggtctt ccgcggcgct ttgggcagga agccttttgt
    20161 ggccgtgcgt gatgtgtccc tgacggtgcg ggagggggaa gtgtacggcc tcatcggtcc
    20221 caacggctgc ggaaaatcca ccacgatgaa gacggtgctc ggcctgctgg cgccgacgcg
    20281 cgggcggacg tttgtctttg gccgctcaag cacggaggtg gcgagccggc aggcggtggg
    20341 ctttctgccg gagaacccgt atttctacaa gcatctcaac ggcctggaga cgctgctttt
    20401 ctacggccgg ctgtgcggcc tgggcggcgc ggagctgagg gaccgcgcgc gggagatgct
    20461 ggcgctgacg ggtctggagg atgcggcgga ccggcgggtg gcgggttatt cgaagggcat
    20521 gctccagcgc ctcgggctgg cgcaggcgct gctgcatcgc ccgcggctgg tggtgctgga
    20581 cgagccgacg gcgggtgtgg acccggcggg ctcgcgcaag atacgcgacc tcatcctggg
    20641 cttccgcggg cagggcatca cggtgctggt gacgagccac ctgctggagc agatgcagga
    20701 ggtgtgcgac cgcgtgggca tcatggcgca cgggcgcatg atgcgggagg ggcgcgtgga
    20761 cgagctgatc gcggtggaaa accagacgga gctggtgctg gaggatgcca ctccggagct
    20821 gctggcggag attcgcgcgc tggtggagcg ccagggcggt gcgcgtctcg tgcgcgcggg
    20881 gcagccgcgc acgactttgg aaaaactttt cctggaggcc acggaggaca cgctttgatg
    20941 aaccacgccg ctttttcccc cgcccgcgtc tggacgctgg ccgccgccac ggtgacgcaa
    21001 ctggtgcgca tgaagatcct cgttttcctg ctgctcttct gcgcggtggt ggtggcggcg
    21061 ggcttcgcct ttccggtgct caacccggag cagcagttga agctcctgaa ggatgtgtct
    21121 tttggcgcct tgcagatttt cagcctcgtc atcggcatcg tggccacggc gctgctactg
    21181 ccgcgcgact tggaggaccg cacgctttac accatcctct ccaagccggt gccgcggcat
    21241 gagtacctgc tgggcaagct gctcggcgtg ctggcgctca tcggcgcggg actgctggtg
    21301 atggacgcgg ctttcagcct ggtgctgtgg ctgcgggaga agctgctgct ggcgcagatc
    21361 ctggccgggc tgaaggcgca gacgccggag gcggtggcgc aggtgcagga gctggtgggc
    21421 aaacaggggc tgacgtggaa tctgcacctc ggcgtgctgg cggtgtttct gaaagcggcg
    21481 gtggtgacgg cgctggcgct gatggtgtcg tgcttcgcga gcagcacgct gttcacggtg
    21541 gtggtgacgt tttgcatcgt catcatcggc cacggccagg ggctgctgcg ggagtatttt
    21601 ctccagggga agatggagcc gtttgtggaa aaggggctgt ccgcgctgct ggcggtgctg
    21661 acaccggacc tgggcgtctt tgatgtgata gacaatgtgg tgaacggcca ggtggtgggc
    21721 ccgggcagcg cgggttacat gacgggcatc gcgctgactt acatcaccgg ttacatggtg
    21781 gtgtcgcacc tgctgtttgt ggaaaaggaa ttgtgatgaa gcgccccctc caggccctgc
    21841 tcgtgctcct gctgctcggc gcgctgaagc tgccgctgga gcaggccgcc acggcgcatc
    21901 tgcgcgcgga gaagctgctg acgccgcgcg tggacctggg cctgcgggag aacctggggc
    21961 agatgggctt cgccgcctcc ctcggcgggc tgcgctcgct ggtggccagc atcacttatc
    22021 tgcgcgcgtg cggcgcatgg gagaatgtgc ggtggggggc ggtggacagt cttttccaac
    22081 tgaccacgcg cctgcagccg cgctacacgc attactggga cgaggcggcc tggcacatgg
    22141 cgtggaatgc ctcgtcccat tacttgaaca atgagaagct gaaccctgcg ttgcgcgaaa
    22201 agctctccta cgagcatgtg ctgcgcgggc gcgacatcct gctggagggc ctgcgcatga
    22261 tcccggatga cgcgcgcctc tggcacacgc tggcggagct ctacgagcgg cgtgacatcc
    22321 agcccgcgaa ggcggcggaa tgctacctca aggtgggcga actgaccggg aacctgcgct
    22381 tccgcaggct ggcgggttat caatacgcga aggcggcgga ccgcgcgctc cagctcaagg
    22441 gctacgagct gctcatggag tcataccgcg ccaaccagcg cccgccgagc ctgatcaaca
    22501 cgatcaagga cctggaactc gcgctcagca tccccgccat gctgcgcatc cccgaggcgc
    22561 gcgtcgcgcc gggcgcggtg cggtgaggtt ttgaaaatca cacgcagcgc gccgctgcgc
    22621 cggtctccat ggcgcgccag acggcatcca cgacgcgcat gtagcagagg ccttcggtga
    22681 agtcgggtcg cgggcgcggt gcggaggggt tgcgcacggc cccaatgaag tcgcgctcga
    22741 cggcccacgc gcgctgcatt tcctgcggca cggccacagg ctccagagaa ccgccggtct
    22801 tgccaagcgc cagctcctcg gtggcgaagt cataaacaag cgtgccctcg ctgccgaaga
    22861 tccacagctt gtccgtcggc gcgtgcgcgg ccacgccgct gaagagcagc gcggcctcaa
    22921 gaccgccgct gaattcggca ttcacatgca ggaagtccgg cgtgccgact tcgtaagcgc
    22981 cacgcaccgg gatgcccacc ttgccgcgcg cctcgacttc ggtgatgtgg ccgagccagt
    23041 gctggagcac ctcgatgtaa atgccgagtg tgaggatctg aatgccgctg atctccgtgc
    23101 gctgacgcca gtgcgcgggc tgtgtggcgg gcagccagtc ggagttgaag ctgtgcagca
    23161 gcgcgtgcag cggcacgccg atggcaccct cggccagaag ctttttcacc atgaggccgc
    23221 cgcgcattcc atgcggagcg gggcagatgg cggtgacgag ttcggggaag ctgcgggatt
    23281 tctcccacat gcgctccgcc tccgcgagag tggcggccat gcgcgcctgg gtgaagacgt
    23341 gcttgccgcg ttcgagggcg aaacaagtcg cgtcatgatg caggcaggga tgcgcgccga
    23401 tccagacgat gtccacatcg tcattgtcca ccacgtcctc ccagcgtgag tgcggccgag
    23461 cctccggcgc atggtcgtgg cagaaagttt ccgcgctggc cagggtggag tttgccaccg
    23521 cggtgatgct gatgccgggc agcgcgcgca ggccggggag gtggcgctgt ttgacgatgc
    23581 cgccggcgcc gacgatgccg atgcgcaaag gatggaggtc actcatggcg gcggacattt
    23641 cacaggatgc gcgcccgcgc aaacccgcgc tcgcgctttg tgcggagctt gaaacatccg
    23701 cggagtgtcc gcgttttccc gcgcccatga aactcgcatc cctgctcctg ctcaccgccg
    23761 tctccgccgc gcccgccgcc gatgaaaagc ccctgctcgc cattcccggc aaggtcattt
    23821 atgaaaacaa gctcgacacc gcgccgggcg cgccgtggaa gatcgcgaaa ggcaagtggg
    23881 agctggtgga cggcgtcatg cgcggctccg agctggaggc tgacaatcac ggtgcggtgg
    23941 cgcgcctgcc caaccagctt tccaatttca tcatcgagta tgagttcaag ctggagggcg
    24001 cgcgctcgac cagcctttcc atcaacgcgc tcaaggacca catggcgcgc atcgccatca
    24061 ccccgcgctc ggtgaccatc cagcgcgatg acaatgacca cgaggggccg gacaaggcgg
    24121 tggtctttgc ccgtttcccc gccgagctca gccccggcgt ctggcacaag gtgcgcctgg
    24181 agatggtgga tgacaaaatg ctcggccagg tgggtgaact gaccgcctgg gggaccagcg
    24241 agcttttcac cacgccaaag gcagcgcccg gattcacggt cagtggagag tcggtggact
    24301 tccgcaatct cgtcatccgc gaggcgacac tgaaccccgc ctgggaaagc gtgatggcaa
    24361 cgctgccgaa gcccggcgaa aaagtggccc cggctccggc aaagggaaaa ggcaagggca
    24421 agaagaagga gtgaggcttt cccgaaaaac ggctgccgcg ccttgcgcgc gtgccgcatc
    24481 cgtttagaga gccttcatgt catccccgga gatcaacatc cacaaagtcg ccaccctggc
    24541 ccgcctcgcg ctcagcgatg aggaggccgc gcgctatcag gagcagctcg ggcagatcct
    24601 gcattacata gacacgctca gcagccacga cctggaaggt gtggagccaa ccgcgcacgc
    24661 catgcccgtg ctggatgtca tccgcgccga tgaaacccgc cccggcttca gccaggacga
    24721 ggcgctggcg aacgcgccgc gccgcgtcat gggccagttc cagatcccca aggtcatcga
    24781 gtgacgccag ttcaacgagt cttccgcatc ttttccaccc atgtccctcg cctcccagac
    24841 tctcgccgcg ctgcgccggc agatcgccgc caaggaaatc catccgcgcg acatcgtgac
    24901 cgacctcgcc gccgccatcg aggcgcgcaa tggcgaactt ggcgcctacc tgagctggga
    24961 cgcggacaag gcgctggccg aggcggagca ggtggccact tccctgcccc tgggcggcat
    25021 ccccatcggc atcaaggaca acatcaacgt caccggccag ccctgcacct gcggctcgcg
    25081 catgctggcg gaaaactaca ccgcgcccta tgacgcgggc gtcatcacga agctgcgcgc
    25141 aggcggcgcg gtgcctttcg gccggatgaa catggatgaa ttcgccatgg gatcgagcac
    25201 ggaaaactcc gcgctcggcc tcacgcgcaa tccgcgcgac ccgcggcgag tgcccggcgg
    25261 ctccagcggc
//
LOCUS       DAOMYJ010000056        24266 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Prosthecobacter sp. isolate AMR_MDS_5629
            NODE_748_length_24266_cov_21.378877, whole genome shotgun sequence.
ACCESSION   DAOMYJ010000056 DAOMYJ010000000
VERSION     DAOMYJ010000056.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867850
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prosthecobacter sp. (wastewater metagenome)
  ORGANISM  Prosthecobacter sp.
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Verrucomicrobiaceae; Prosthecobacter.
REFERENCE   1  (bases 1 to 24266)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 24266)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.30
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:34:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,742
            CDSs (total)                      :: 4,684
            Genes (coding)                    :: 4,676
            CDSs (with protein)               :: 4,676
            Genes (RNA)                       :: 58
            tRNAs                             :: 55
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24266
                     /organism="Prosthecobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_748_length_24266_cov_21.378877"
                     /isolate="AMR_MDS_5629"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1965333"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(26..286)
                     /locus_tag="PLP58_08010"
     CDS             complement(26..286)
                     /locus_tag="PLP58_08010"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HRJ08495.1"
                     /translation="MIRLESISKQTSTRMLFIEASAALNRGEKVGLVGPNGAGKTTLF
                     RMITGEEAPDEGNVSVDRGVTIGYFSQDVGEMAGRSAVNAVM"
     gene            complement(483..3710)
                     /locus_tag="PLP58_08015"
     CDS             complement(483..3710)
                     /locus_tag="PLP58_08015"
                     /inference="COORDINATES: protein motif:HMM:NF016260.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translocation/assembly module TamB
                     domain-containing protein"
                     /protein_id="HRJ08496.1"
                     /translation="MLAKVISTALSSPEGQVRIGAVDGPLSSNAQIRDIEISDREGPW
                     LRVDSARLVWRRAALLSRRLEVEQLEIGRIEVLRKPVPAVRQAAGNDEPLLPELPVKV
                     VVAAFSVGELVLGAPIAGSPARLAVTGNLSLGAPSEGLQAAIALRRRDRDGTSDLRLQ
                     FLPKGETLDLVLQHQESPGGLVAHVLDIKDRPAVSLDLQGRGPLDAWRAQLAFSAGDA
                     GRATGRATITRQGASRRVDLDVAGQVQGLLPPVAATIFSGETSITGQLDLADDGAIAL
                     PGLRLASTQAELTVAGTLGSDRRLDVQANARALPGDRTSTPRLAALSGLMLDVRARGT
                     LDAPDIDGRIEAEISRLEAFADDLGLSIRGGAKISAAVKGRPLDRTLDASLAVIGRTL
                     TFGNAQLTALLGSQAALSGKASLRGDRVAIENLALKARRVDAVLSGSVETAASATRGA
                     ITLAGKLDGRALSGQTSFVAERGGALDIASLDLRVAGGKVAGALKVAVGGLVTGAVSV
                     DFPDLGQFSAALGTRLRGVLSAQATLTAPQGAQQAAIKAKGRGLSGFGAALASLDGEA
                     MLHGDLARPAGTLRATLAGLQYPDLQRAGVPRLDIATTGALMGDRVRLDASVRGGGQI
                     ALKVSGDAPFSTRGGLGLKVTGTVDAALANSRLTGGAQRVAGRVALDASVRGTLAEPR
                     FEGAAVLSNGSFSDLLQGFRLTNVSGRIAGAGTSLRVENVTAVTPGEGRIAVNGTVHV
                     DPARGFPANLRVTGENARLLHNEVVRLVAGLDVAVTGPLASAPVVAGQVNVVSFDVTV
                     PDRLPVDSAPLANAKHLSPPEQTRERLRLAEREGQRKARRKAGAAGNARLNLRIVAPS
                     HIFVRGRGIDAELGGDIALSGPLLAPRANGAFELRRGQLSMLTQRLEFSRGRVTFLGD
                     LIPDLDFTAATLAGSITAKVSVTGRADSPSFALTSEPQLPPDEVLSRLMFEQAVGSLS
                     PLQAVQLAQAVARLTGRGGPDFLEKTRQALGVDTLDVTAGKDGPTVGASRYITRNIRL
                     GVKAGATPEQSGVTANVDVGRRIKLQGETGASGKTSVGIAAEIEY"
     gene            complement(3800..4135)
                     /locus_tag="PLP58_08020"
     CDS             complement(3800..4135)
                     /locus_tag="PLP58_08020"
                     /inference="COORDINATES: protein motif:HMM:NF013283.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BamA/TamA family outer membrane protein"
                     /protein_id="HRJ08497.1"
                     /translation="MGADDFWGYEYRSIGPRDTLGRLVGGRSLFEASLEARIRATETI
                     GIVPFVDVGAAFSSAAPSFDETLRFGLGVGLRYHTAIGPIRVDVATPLDRRRGEKPVA
                     LYISLGQAF"
     gene            4339..4932
                     /gene="cysC"
                     /locus_tag="PLP58_08025"
     CDS             4339..4932
                     /gene="cysC"
                     /locus_tag="PLP58_08025"
                     /EC_number="2.7.1.25"
                     /inference="COORDINATES: protein motif:HMM:NF003013.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004020 - adenylylsulfate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006790 - sulfur compound metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylyl-sulfate kinase"
                     /protein_id="HRJ08498.1"
                     /translation="MENCTAPDFTVSRADREKLLGQRGMIIWLYGLSGAGKSTLANEI
                     ANVLHHAGRLTLHLDGDVLRHGLNRGLGFTEADRVENLRRAAEVAKLAVQTGAVVLCS
                     FITPLNSMRRMVREIIQNGDLLDVYVSCSFSTCASRDVKGLYAKAQANALANFTGKDS
                     AFEEPDYDPGLVLDTDHLDIPECTRLLHEAVSGRIGL"
     gene            complement(4933..6381)
                     /locus_tag="PLP58_08030"
     CDS             complement(4933..6381)
                     /locus_tag="PLP58_08030"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:NF013146.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MraY family glycosyltransferase"
                     /protein_id="HRJ08499.1"
                     /translation="MNAPAENFNPDLVLTITWSELAVHAVLVLALSASGAWWLSHRRA
                     GFGMDAPDGRRKLHETPVSRLGGLPIYLATVIGLTVVIFLGNHGFRAWAPLLICNTLI
                     FTVGLADDLKPLGARVKLVGQLGTALILYSLGVSIDVLSNPFGEGGIHLGWWSLPLTL
                     VWLVAIPNIINLIDGMDGLAAGFGLFLSLTLAFIGYFSFQTDVMIASALMAAALGGFL
                     FFNLPPAKIFLGDGGAYLIGFFIASVSLQSSNKGSVIAALLVIVIALGVPILDTFFAI
                     VRRTMRGVPVFSADAEHIHHRLILLGYSKGRALAVMYAVCLVLSLVGISILLTKGIAL
                     PVAGAVMFLLAVGAARYLGYVRSWTRLREQVGQALERRRKYEHVQAHARALEFDVEVC
                     GSLEEFAGLLRERLRWAGFSTASSEGARPVKISLTGGGEVLLHHKQDADAEEHWLRRA
                     DCFVTLLERCEERWGSLPDVLQRVPVEGRAAN"
     gene            complement(6393..7610)
                     /locus_tag="PLP58_08035"
     CDS             complement(6393..7610)
                     /locus_tag="PLP58_08035"
                     /inference="COORDINATES: protein motif:HMM:NF024968.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HRJ08500.1"
                     /translation="MKVLLLNQCFHPDHVSTAQHLTDLALGLRAAGHEVTVVAASRGY
                     DDPARRFPVRETWQGIEIHRIWTPGLGKKAKWRRFTDFAIFWMNATLILMRLPRFDVT
                     VCLTSPPLISTLGTVIAALKGGAVVPWVMDLNPDEAVAAGWLRAGGLAERVLSALQKW
                     SFRRAARIIALDRFMAARIEAKGVPPVLIHTDPPWSHDDQVRFDPQAREAFRAEHGLA
                     GKFVVMYSGNHSPCHPLDTLLEAALRLRGEDDVRFLFVGGGSEMGKVKAFRETHALTG
                     ILTLPYQPIEKLAASLSSADLHVVVLGDAFAGIVHPCKIYNILILGLPFLGIGPEQSH
                     LNDLAGRLREPGFAFQARHGDVDGVLACLGKARAAGFRGCSEESRQVAAEYSREILRG
                     RMVRILEQTAQAG"
     gene            complement(7625..8614)
                     /locus_tag="PLP58_08040"
     CDS             complement(7625..8614)
                     /locus_tag="PLP58_08040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007414383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HRJ08501.1"
                     /translation="MENDPLITVCGAGGFIGGALVADLRAQGFVRLRAVDQKPLDEWY
                     QRFDDVENLRLDLSGKSACETAAKDTHTLYNLAADMGGMGFIENNKALCMLSVLINTH
                     LCMAARDAGVARYFYASSACVYNADKQKSYDVPALKEADAYPAMPEDGYGWEKLFSER
                     MCRHFREDFGLQTRVARYHNVYGPHGTWDGGREKAPAAVCRKVALAKRSGAHEIDIWG
                     TGEQTRSFMYITDCVQGTQMLTQSDFIEPVNIGSAEMVSINQLVDIVEEIAGVKLKRS
                     YKLDAPKGVNGRSSDNTLIKEVFGWEPSTRLRDGMEKTYAWISDEIAAGRASL"
     gene            complement(8678..9421)
                     /locus_tag="PLP58_08045"
     CDS             complement(8678..9421)
                     /locus_tag="PLP58_08045"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HRJ08502.1"
                     /translation="MKRRLHLVVPCFRESARLPRFLPQLCAEMDEIAGVSVLVVDDGS
                     GDSEAAATRKVVEDLRGRHACLLPLMELPENLGKGGAVYAGWAAHEGAEWLGFVDADG
                     SCGALEAARLARMAMTDCASPSALFASRVKMLGRRVERLFKRHLLGRVYATLVSELLR
                     IPVYDSQCGLKLVPRAAYEKAAGHLQVHGFAFDVELMAALLDAGCPVAEAPIDWRETP
                     GGKVSLLRDSWRMALDVWRIRRRRANGWK"
     gene            complement(9444..10193)
                     /locus_tag="PLP58_08050"
     CDS             complement(9444..10193)
                     /locus_tag="PLP58_08050"
                     /inference="COORDINATES: protein motif:HMM:NF016908.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FkbM family methyltransferase"
                     /protein_id="HRJ08503.1"
                     /translation="MKFDLNPLNKLKLKLWLARRLNFRYCHYHGYRFFWDRVCYPELE
                     SVARPVIFDVGANVGQTAAGFREHFPGAEIHCFEPVPASFRALQSHTAGMDIRCHQLA
                     MSDTPGRVKVRLLSDDPEFTMNSLNHEAAADAEGPGVAWFDVVTLPEFCARHNMEVVH
                     LLKIDTEGRDLHILRGAEAMLRAGRVWSIVLETVPAPYQDNGLVSLHEFTDYLRPLGF
                     ELYSLFDIMHQHGGRVAFMNALFKHQSACAR"
     gene            complement(10227..10988)
                     /locus_tag="PLP58_08055"
     CDS             complement(10227..10988)
                     /locus_tag="PLP58_08055"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009959364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HRJ08504.1"
                     /translation="MRHELVTPLILTWNEEPNLARVLARLGWAREIVVVDSGSTDATC
                     EMVCAHPGARLLTRTFDDHTSQWNFGVDAVTTPWVLALDADYVIGPDFENELAALDDQ
                     ADAYEAGFRYLTFGRALRGSLYPPRAVLFRKDRCRYAQDGHTQLLHVTGGTGRLSTLI
                     DHDDRKPLTRWLSSQDKYSLLEAEKLLRADPAALRLQDRLRLTGWAAVPAVAVHTLLV
                     RGVILDGWRGWYYTLQRMLAEMLLALRLLEKRLNK"
     gene            complement(10985..12967)
                     /locus_tag="PLP58_08060"
     CDS             complement(10985..12967)
                     /locus_tag="PLP58_08060"
                     /inference="COORDINATES: protein motif:HMM:NF013530.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HRJ08505.1"
                     /translation="MKILVLASSSFAARNLPAALLAAGHEVWTFNRSQIENSGPRDLH
                     GCYDRLAEITGTAMDGVCDVLINFAIVKNGSIEENISLTDQVMQAAKKLHVRRFIHIS
                     SISVLPSITGVLNEDAEAVEVRWKGIYSRVKAAVEGHIIDTWREGDLDVVRPGFILAP
                     GLVDSMVGTGKQLPTGHVLGLGNQRTVIMLVHRDAVDQALVKIAGSASNSSSPKLKKY
                     MLVAPNAPNRREYLDFQCRELGRGWSTLHFPAWMWRLALAAASIPISLIKRRLIRLDL
                     LFMHNLNVRTYDCTRTSRDLDLEMSFDWRETLRNLVHLKPSPPWPPTIPLPARSKPGF
                     LAYFGMGRIVGQKHLPGLARLGFKGGIFWSDPALTRAPACAGIKIETYPGIPQDASHA
                     IITAPVNARPNILDGLAETVSQVLLEKPFATSAAMLERMMEYIKSRDVFVLHNYRFKP
                     NLMRMRAHLRNHPPGALRAVSLHFETPSPANEASSWMKQEWRNRIVLTDYAIHYLDLC
                     WMFCQGEMQVNRCLVTRNDRGELETLSASMTFDGIPCDILIRSGGHQRHCIIRHHFQN
                     YSTETRFFPDVFVAMTGGQGPLDDAQLALRGFTRTVAKVFEKLGLRVTDRSHDTVLES
                     FTGHGDSSPLQELSLENLLTFYRRLVMLADRVYQEA"
     gene            complement(12964..14133)
                     /locus_tag="PLP58_08065"
     CDS             complement(12964..14133)
                     /locus_tag="PLP58_08065"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HRJ08506.1"
                     /translation="MKILHCVASLAPRHGGPTVAVFSMVRAQRELGVDAVILSSDDDL
                     GGRLNVPLRRWIEHEGVPVFFLPRVRARQHTLAGFTFTPEYPAWLRAHVREYDFAHIH
                     TVFSHPANSAMRVCRNAGVPYCVRPLGQLCHWSLLQRRLLKQLQLALVTRRNINGARF
                     IHCTSEMEARETASEGFSSPCLVLPHGIVMPPRLVDARERLRKLLRLPADRHVVTFMS
                     RIHPKKGIEMLLEAAAKLPACFDLAIVGTGDEAYLNRLKQATEQSLPGRVHWLGFKEG
                     EEKWLVLQGADMFVLPSYSENFGIAVLEAVACGLPVVISDQVALQDEVKKHHLGIVTP
                     LSADALAEAMKDLLASSTERKEISSRATVTAAEHFSWESAAQRLTAACLAHLSAP"
     gene            complement(14133..14966)
                     /locus_tag="PLP58_08070"
     CDS             complement(14133..14966)
                     /locus_tag="PLP58_08070"
                     /inference="COORDINATES: protein motif:HMM:NF016908.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FkbM family methyltransferase"
                     /protein_id="HRJ08507.1"
                     /translation="MILEHLRSLLRATGMAGRLSPLMTRLENFVLARHGKIVQRSMSP
                     ETSEIVSASGTVIRAGPPRHYADMMLSRHELPTLVACVNVFAPVLKNGVAWDVGGNAG
                     FYSTLLSQLVGHEGSVYAFEPVPATFHGMCANLSAAGACNVTALNLALSDHEGMVPIS
                     FDPASDTTSSIENTTGMSCMEVRAATGDGLVASGECLPPTMLKIDIEGHELKALKGLE
                     KQLARAECRAVLCEIHYAILAANGEHQAGAKVRKALTNAGFDRIYFISRSHLIATKNL
                     F"
     gene            complement(14988..16778)
                     /gene="asnB"
                     /locus_tag="PLP58_08075"
     CDS             complement(14988..16778)
                     /gene="asnB"
                     /locus_tag="PLP58_08075"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR01536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HRJ08508.1"
                     /translation="MCGIAGIFQSEPAPIEAALEKLRAALLHRGPDDEGQWLSPSGLA
                     GLAHTRLAVLDLSPAGHQPMITADGRFAIVFNGEIYNFRELRAELEKTGAEFRTRSDT
                     EVLLRLYEREGAPMVRRLRGMFALAIWDEAERTAFLARDPFGIKPLYYSHQAGRLAFA
                     SEVRALQSAGLADSSLDAAAMMRYLESGSVAEPATLLKEVRALPAGHSMIWKAGAPRM
                     ECHWRPVFSPGPMSAGEAAEKTRAALLDSLDHHFVSDVPVGLFLSGGVDSTLLAVLAR
                     EQGRAGLESFSIGVDDARLDESSVAARTAAHLGLRHHELRLDAAGMAESFPRFLRHMD
                     QPGIDGFNTFMVSGFARAQGMKVVLSGLGGDEIFGGYASFEKVPRLAHAAGWLRKLPG
                     STMMGRWMETTLPRANLRRVGDLLQRGPGMTEAWRVFRGIFTRAQARRLTALYSGCDE
                     ASLSPETALEADTPDERDQASLLEMTLYMRNQLLKDSDVMSMAHGLELRVPLVDRALF
                     ESVAHIPAALRLRAGKQLLLDAVPEIPDWVAGQPKRGFLFPYEKWLAADWGGAFEQAR
                     ADIRDPRAPWYQQWCVFMLRQWLESAHSHT"
     gene            complement(16785..17921)
                     /locus_tag="PLP58_08080"
     CDS             complement(16785..17921)
                     /locus_tag="PLP58_08080"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HRJ08509.1"
                     /translation="MSTRLIHVWAPGIRQGSGGIQAFSRDAVRALLAAYPEARLHVHV
                     KNDAPGEDDPLFAERVTWSSSRAFHRVLRTLHFALSGILRTLLMRPCLVLSTHVNFLS
                     AVHLLKPGACRTAVVLHGIDAWNLRAGAKLRALRKTDAVISVSRFTRDQVAKLPGMEG
                     ARMFVLPNTFQTGAFQPGPKPEALLERHGLKAGQPVLLTVSRLEESERYKGHQEVIEC
                     LPELLKEFPGLRYVIAGRGGYAAELKRLVKKHAPEEAVIFAGFVSNTELADYYRLCDV
                     FVMPSRGEGFGIVFLEALACGKPVVAGNADGSADAVDQGRLGVLVNPQSREELTEALR
                     SLLAGTHPNRLLFEPAKLHEDVVRQFGPEAFRGRLKAILDEIWD"
     gene            complement(17918..18865)
                     /locus_tag="PLP58_08085"
     CDS             complement(17918..18865)
                     /locus_tag="PLP58_08085"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family A protein"
                     /protein_id="HRJ08510.1"
                     /translation="MTQITAIVPTHERLDMVSRTLERLLACDPAPAEILVHVDGGNAT
                     LYSEIEARFPGVRCLLSEKRLGPGGSRNRLIAEAAHDWVANFDDDSYPAHRDYFARAA
                     ELARAFPDAAVLSAASHALEWARLVLQRISVFSGCGCVFNKRWFARTRGFVPLPIAYG
                     MEEVDMSLQLHALGGRMIHDPLLRVNHEPPADRARDEQRVAAVVLANTVLLPWLRYPV
                     WLWPAGVAQVLSKIRWMLAHGQTGAVWSGLQMTPTHLLAYGGHTGRVSARALLSWLRL
                     RRAHQDLQDANSLPHGFVDGAARRITRSTGEGQDAGPAS"
     gene            complement(18881..20332)
                     /locus_tag="PLP58_08090"
     CDS             complement(18881..20332)
                     /locus_tag="PLP58_08090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ08511.1"
                     /translation="MTTPDIFLAASLSKTTLLGVGAGGALVVAIWSFYHWRTAVKAAL
                     VVALLEGALRKWAFPQGQELVYFLKDVFLFGAYLKFFLAPDLDVRSHRLRIPTGLILA
                     LCVIVAAFALNPNINSAIISVFGLKAYFYYLPLLFMMPFLFRTEEELIKQMTWFTLIA
                     LPICLLGLAQWRAGPDSILNVYAQRDGDRITNDGFGAGMGDRARITGTFSYITGHATF
                     IVFFVTLCLVMLTLREARWKWVIAWAILPLLAVNSLMSGSRSGLYTAAFIIAGMALPA
                     FAGRIGQGTPIRTILITSITAGGLIISYWFYEAFFYMSVRTSTAADTIYERTIGHTHE
                     AMSFAIEKAGWFGYGLGLTHPATASLKNFLGLAGPAKQPVGVEAEAGNVWMDVGAFGF
                     VAWYAFRFLLVWLCWRSYTLARTPVQKSFALAAFLLSLPYLSMQVVMNHTASILLFAF
                     CGLAMLPTLEPLVMLRHRRPQRTGTPSRQPAHG"
     gene            complement(20340..20948)
                     /locus_tag="PLP58_08095"
     CDS             complement(20340..20948)
                     /locus_tag="PLP58_08095"
                     /inference="COORDINATES: protein motif:HMM:NF016908.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FkbM family methyltransferase"
                     /protein_id="HRJ08512.1"
                     /translation="MDVGTHWGMFTLAALHYGGPEARCLDIEASDAAARVLKDNLALN
                     NASGRVTLVNAACGDKPGTLSMLTTGAGGADYFVVPQETRPDCVTVEQVTVDQMALSH
                     GFAPTHLKIDVEGYEMEVLRGARETLAASKPLIFLELHGDLIRARGSEPGEVLQLLKE
                     SGYTAWMDVREQALSLESAQERGCNLRMIALHPEASASSVQA"
     gene            complement(21206..22222)
                     /locus_tag="PLP58_08100"
     CDS             complement(21206..22222)
                     /locus_tag="PLP58_08100"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HRJ08513.1"
                     /translation="MKVVIHSPAFHPMIGGLEAFAAMLAGGLHERGHEVVVLCQSDLP
                     AGEAEPFPFRVVRRAGFQAMARWMAWADVVIHVQISLKGLLPWLITRRPLLVSHQTWL
                     REEGGAPLTWQTRLKRLACRLAVNVTCSAAIGRDLGLPHIVIPNPYDDALFHPRPEVA
                     RDRDLLFVGRLVSDKGADLLLEAVARLAAQHMHVSATITGSGPEEPALKSQAEKLGIQ
                     DRVRFTGPLRGEALAREMNAHRVLVVPSRWAEPFGIVALEGAASGCVVVASDGGGLPE
                     AAGPCGIIFESGNVENLARCLKTALEQGAPDAREHLQRHQRARIVASFERMLLEMTTP
                     HTTS"
     gene            complement(22219..23046)
                     /locus_tag="PLP58_08105"
     CDS             complement(22219..23046)
                     /locus_tag="PLP58_08105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ08514.1"
                     /translation="MIWVLHYSGYGYAKRGAPLGLLRSMREMRRERPQTRLVTMFHEL
                     FAGGAPWTSAFWFSMLQRHAAAGLARLSDAAVTNRRDSAVWLRKHLTKNADVSALPVF
                     SNFGEDAAPKLPMARPARLMAFGGGPARRPDFWLQVEAAMNQLSLSELVMVSHPITVP
                     ESIRARFVVRQTGVLEPAAMAEELRSSRCGILDYNPDYLGKSGVLAAYAAHGVVPVLT
                     QGHGVISEGLTEGRHFLSTADLKSTHDLEGVQNRLREWYAPHSLEMTAREYVRVATA"
     gene            complement(23306..24232)
                     /locus_tag="PLP58_08110"
     CDS             complement(23306..24232)
                     /locus_tag="PLP58_08110"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HRJ08515.1"
                     /translation="MNDAFDRWAARRFAQNPARILVGTETCAWHSFEAAEEIGAVKVL
                     DCPQLHPGLLTEVMREAGDRARLPAAVERDDPAMTTRKQREYELADWRMVYSSMHRRS
                     FERAGFPTGQQVEIPLWVDPSLWFPEKEHREPDGRLKVLFAGSITLRKGIPFLLEAFA
                     LGGGSWELTLVGRVDEALKERFASYQDRVRLLPPQTKAELRRLYAEHDLFVLPSVADS
                     FGFVALEAMACGTPVLISENCGAPAPDAGWRVRPMDAEDLASKIAAYVENPERVMVEG
                     RRGQEFAAQFTPERYRREAGRFFRRLLEGGGA"
BASE COUNT         4847 a         8014 c         7184 g         4221 t
ORIGIN      
        1 gatgtcggag agatggcccg gcgccttaca tcacggcgtt caccgcactg cgcccggcca
       61 tctcgccgac gtcctggctg aaatagccga tggtgacacc gcgatcgacc gagacattgc
      121 cctcgtccgg agcttcctca cccgtgatca tgcggaacaa cgtggtcttg ccggcgccat
      181 tcggaccgac cagtccgacc ttctctcccc tgttgagggc cgccgaggcc tcaatgaaca
      241 gcatgcgcgt gctggtctgc ttgctgatgg actcgagacg gatcatgcgc ggacaaattc
      301 cgaggtgcag gaaggggtag gcaggcgcca ggcccgggcc gacctagcgc ccagatacct
      361 gcgccgccag gaccgcgcaa tcggcgatct ccggatcgac ccgaagcacc aaagctaatc
      421 ctggcgcgtc ttcgaggccc cggcacttca gctttccacg gcgccgactc aaacgtgcag
      481 cgtcagtact cgatttccgc ggcgatgccg acggaggtct tgccgctggc accggtttcg
      541 ccctgaagct ttatgcgtcg cccgacatcc acattcgcgg ttacgccgct ctgctcgggc
      601 gtcgcgcctg ccttcacgcc aaggcggatg ttgcgggtga tgtagcgcga cgcgccaact
      661 gtcggtccgt ccttgccggc cgtgacatcc aacgtgtcga cgccgagagc ctgccgcgtt
      721 ttctccagaa agtccgggcc accgcggccg gtcaggcgag ccaccgcctg cgccagttgc
      781 accgcctgca gcggcgacaa cgatcccact gcctgctcga acatcagccg cgacaggacc
      841 tcgtccggcg gcagttgcgg ttccgaagtc agcgcgaagg acggcgagtc cgccctcccc
      901 gtcaccgaaa ccttcgcggt gatgctgccg gccaaggtgg cggcggtaaa gtccaggtcc
      961 gggatcaagt ctccgaggaa ggtcacccgg ccgcgcgaga attccaggcg ttgcgtcagc
     1021 atgctgagct gtccgcgccg cagttcgaac gcgccgttgg cgcgcggcgc caggagcggc
     1081 ccggacagcg cgatatcccc gccaagttcc gcgtcaatgc cgcgtccacg cacaaagatg
     1141 tggctcggcg caacgatccg aaggttgagg cgagcgttgc cggcggcgcc tgccttgcgc
     1201 cgcgccttgc gctggccctc acgttcggcg agacggagcc gctctcgcgt ctgttcgggc
     1261 ggcgatagat gtttggcatt ggcgagcggc gcggaatcaa cgggcagccg gtccggaacc
     1321 gtgacatcga acgacacgac attgacctga ccggcgacga caggcgcgct cgccaacgga
     1381 ccggtgaccg cgacatcgag accggccacc agccgcacga cctcattgtg cagcaggcgc
     1441 gcattctctc cggtgacgcg caggtttgca ggaaatccac gcgctggatc gacgtgcacg
     1501 gttccgttga cggctatccg tccctcgccc ggggtgaccg ccgtcacgtt ctcgacccga
     1561 aggctggttc ccgcgcccgc tatgcgccct gacacgttgg tcaggcgaaa gccctgcaac
     1621 agatcggaaa acgagccgtt gctgagaacg gcggcgccct cgaaccgagg ctcggccagc
     1681 gtgccgcgca cggaggcatc cagtgcaact ctcccggcca cccgctgcgc gccacccgtc
     1741 aaccgggaat ttgcaagggc ggcatcgact gtccccgtaa ccttgagacc aagcccgcct
     1801 cgcgtcgaaa acggagcatc gccgctgacc ttcagggcga tttgcccacc cccccgaacc
     1861 gatgcatcga ggcgcactct gtcgcccatc agcgcgccgg tggtagcgat gtcgagcctt
     1921 ggcactccgg cgcgctgcaa gtccggatat tgtagccccg ccagcgtcgc tcgcagggtt
     1981 ccagcgggcc gggcgagatc gccgtgcagc atcgcctcgc catcgaggct ggcgagtgcc
     2041 gcgccaaagc cggaaaggcc gcgccctttt gccttgatcg ccgcctgctg cgcaccctgc
     2101 ggcgccgtca acgtcgcctg cgccgacagc acgccacgca agcgcgtgcc cagcgccgcc
     2161 gagaactgcc cgaggtcagg gaaatcgacg ctcactgctc cggtcaccag gccgccaact
     2221 gctaccttca aggcgccggc gactttcccg ccagccaccc tgagatcaag ggaggcaata
     2281 tcaagcgcac ccccacgctc ggcaacgaac gacgtctgtc cggacagcgc gcggccatcg
     2341 agcttgcccg ccagcgtgat cgctccacgt gtcgcggagg ccgccgtttc aaccgaccca
     2401 ctgagcacgg catcaacccg ccgtgccttc agtgccagat tctcaatcgc gacgcggtcc
     2461 ccacgcaggc ttgccttgcc gctcagcgcc gcctgactgc ccaatagcgc ggtgagctgt
     2521 gcgttgccga aagtcagcgt gcgcccgatc acggcgaggg acgcgtccag tgtcctgtcc
     2581 agcgggcggc ccttcaccgc cgccgagatt ttcgctccac cgcgaatgga gaggccgaga
     2641 tcgtcggcga acgcctccag tcggctgatc tccgcctcga tccggccatc gatatccggc
     2701 gcatccaaag tcccgcgcgc ccggacatcg agcatcaggc cggacaaagc ggcgagacgg
     2761 ggggtgctcg tcctgtcgcc tggcagcgcc cgcgcattgg cctggacgtc cagcctgcga
     2821 tcggacccga gcgtacctgc aaccgtcagt tcggcttgcg tggaggcaag ccgcagtccg
     2881 ggaagggcaa tcgcgccatc gtcggccaga tcgagctgac ctgttatgct ggtctcgccg
     2941 gaaaagatcg tcgcggccac gggcggcaac aggccttgca cctgcccggc gacatccaga
     3001 tcaacccgcc gcgacgctcc ttgccgcgtg atggtggcgc gacctgtggc tcggcctgca
     3061 tccccggccg aaaaggccag ttgagcgcgc cacgcgtcga gcggccccct gccctgaaga
     3121 tcaagactga ccgccgggcg gtccttgatg tcgagaacgt gcgcgaccag cccgccaggg
     3181 ctttcctggt gttgcaggac aagatcaagc gtctcgccct tcggaaggaa ctgcagtcga
     3241 agatccgacg tgccatccct gtcgcgacgt cgaagcgcga tggccgcctg caacccttct
     3301 gagggagcac cgaggctgag attgccggta accgcaagtc ttgccggtga tccggcaatg
     3361 ggggcgccga gcaccaattc gccaacggaa aacgcggcca ccaccacctt caccggcaat
     3421 tccggcaata acggttcgtc gtttcccgct gcttgccgaa ccgccggcac cggcttgcgc
     3481 aggacctcga tacgtccgat ctcaagctgc tccacctcaa gccgccgcga cagcagcgcc
     3541 gcgcgtcgcc agaccagacg ggcgctgtcg acccgcagcc acggtccctc ccggtcggaa
     3601 atctcgatgt cgcgaatctg cgcgttcgac gaaagcggtc cgtcaaccgc accgatccgg
     3661 acctggccct cgggcgacga cagcgccgtc gagatgacct tggctaacac ggaccgttga
     3721 tcgtcctgcg cgcgcccctc cgtcgccccc accagcatca gcccgacaag aagcgtcgct
     3781 atggctcgga caacgcgcat cagaaggcct gcccaaggct gatgtaaagc gcgacaggct
     3841 tttcccctcg ccggcggtcc aacggcgtcg ccacatcgac gcggatcgga ccgatggccg
     3901 tgtgataccg caatccgacg cccagcccga accgcagggt ttcatcaaaa cttggcgcgg
     3961 cggaggaaaa agccgcgccc acgtcgacga acggcacgat cccgattgtt tccgtggcac
     4021 ggatgcgcgc ctcgagcgag gcctcgaaca ggctgcgtcc gcccaccagc cgccccaggg
     4081 tatcgcgcgg acctatggag cggtactcat agccccaaaa atcatccgct cccagggcgg
     4141 ggcgagaaac cggtcatacg cgacaaaagc ggcggcgagc agcagggcgg tgatcaaaat
     4201 ggatttcatg agggcggctg gcctttacca cacgcgcggc atttcgcatc cgcagtctcg
     4261 cgttttgaga caggcgcggc ggcgcgaagc aaaaatcggc ccttgcaaaa atcacggccc
     4321 tccaagatat ggcagcgaat ggaaaactgc accgcccccg atttcaccgt ctcacgcgcc
     4381 gacagggaaa agctcctcgg acagcgcggc atgatcatct ggctttacgg cctgtcggga
     4441 gcgggcaagt ccacgctggc caatgagatc gccaacgtcc tgcatcatgc cgggcgcctc
     4501 accctgcatc tggatggcga cgtgctgcgc cacgggctca accgcggcct gggcttcacc
     4561 gaggcggacc gcgtggagaa tctccgccgc gccgccgaag tggcgaaact cgccgtgcag
     4621 acaggcgcgg tcgtgctgtg cagcttcatc acgccgctca actcgatgcg ccgcatggtg
     4681 cgggagatca ttcagaacgg cgacctgctc gatgtctatg tgagctgctc tttcagcacc
     4741 tgcgcctcgc gcgatgtgaa gggtctctac gccaaggcac aggccaacgc gcttgcgaac
     4801 ttcaccggca aggactccgc attcgaggag cccgactacg atccgggcct cgtgctcgac
     4861 accgaccacc tggacatccc ggaatgcacc cgccttttgc acgaggctgt cagcggcagg
     4921 atagggctgt gatcaattcg cggcgcggcc ctcgacggga acgcgctgca agacatcggg
     4981 cagagagccc cagcgctcct cgcaccgctc cagcaaagtg acaaaacaat ccgcgcgccg
     5041 cagccagtgc tcttccgcgt cagcgtcctg cttgtggtga agcagcacct ccccacctcc
     5101 cgtcaggctg attttcaccg gcctcgcacc ctctgaactc gctgtggaaa aacccgccca
     5161 gcgcagccgc tcccgcaaaa gaccggcaaa ctcctccagc gagccgcaga cttcgacatc
     5221 aaactccaaa gcgcgcgcgt gggcctgcac atgctcgtat ttccgccgcc gctccagcgc
     5281 ctggcccacc tgctcccgca gccgcgtcca gctccgcaca taaccgaggt agcgcgccgc
     5341 gcccaccgcc agcaggaaca tgaccgctcc ggccaccggg agcgcgatgc ccttggtcag
     5401 caggatgctg atgcccacga ggctcagcac caggcacacg gcatacatca ccgccagcgc
     5461 ccggcctttg ctgtagccca ggagaatgag ccggtggtga atgtgctccg cgtccgcgct
     5521 gaacacgggc accccgcgca tggtgcggcg cacgatggcg aagaaagtgt caagaatcgg
     5581 cacgcccagc gcgatgacga tgaccagcag cgccgcgatg accgagcctt tgttcgagct
     5641 ttgcagcgag accgaggcga tgaaaaaccc gatcaaatac gcgccgccgt ctccaaggaa
     5701 aatcttcgcc ggcggcaggt tgaaaaacaa aaaaccccca agcgccgccg ccatcagagc
     5761 cgaggcgatc atcacatccg tctgaaacga aaagtaaccg atgaacgcaa gtgtcagcga
     5821 aaggaaaagc ccgaaccccg ccgccaggcc gtccatgccg tcaatcaggt tgatgatgtt
     5881 tggaatcgcc accagccaca ccagggtcag cggaaggctc caccatccga ggtggatgcc
     5941 gccctcgccg aacggattgc taagcacgtc tatggagaca cccagcgagt ataaaatcag
     6001 ggcggtgccg agctggccca ccagcttcac gcgcgcgccg agcggcttga ggtcatccgc
     6061 caggccgacg gtgaaaatga gcgtgttgca gatcagcagc ggcgcccagg cgcggaagcc
     6121 gtggttgccc agaaaaatca ccaccgtcag gccgatgacc gtcgcgagat aaataggcag
     6181 cccacccagt ctggaaaccg gcgtctcgtg cagcttgcgc cggccatccg gcgcgtccat
     6241 gccgaaaccc gcgcgcctgt gcgagagcca ccatgcgccc gatgcggaca gcgccagcac
     6301 aagcaccgca tgaacggcca gctccgacca ggtgatggtg agcaccaggt cggggttgaa
     6361 gttttcagcg ggcgcgttca tccagccatg tgtcacccgg cttgcgccgt ctgttcaaga
     6421 atgcgcacca tgcgcccgcg gagaatctcc cgcgaatact ccgcagccac ttgccttgat
     6481 tcctccgagc agcctcgaaa ccccgcagcc ctggctttcc ccaggcaggc cagcaccccg
     6541 tccacatccc cgtgccgcgc ctgaaaggcg aagccaggct cgcggaggcg gcctgccaag
     6601 tcattgagat gactctgctc cggcccaatg ccaaggaagg gcaggccgag gatgaggatg
     6661 ttgtagattt tgcacggatg cacaatgccg gcgaaggcgt cccccagcac caccacatgc
     6721 aggtccgcgc tggacaggga ggcagccagt ttctcgatcg gctgataggg cagcgtgagg
     6781 atgccggtca aagcatgggt ttcgcggaac gccttcacct tgcccatctc gctgccgcca
     6841 ccgacgaaca aaaagcgcac atcatcctcg cctcgcagcc gcagcgccgc ctccagcagc
     6901 gtgtcgagcg gatggcaggg actgtggttg ccggaataca tgaccacgaa tttgcctgcc
     6961 agcccgtgct ccgcgcggaa agcctcgcgt gcttggggat caaatcgcac ctggtcatcg
     7021 tgcgaccacg gcggatccgt gtggattaaa accggaggca cgcccttggc ctcgatgcgc
     7081 gccgccatga accggtccag cgcgatgatg cgcgccgcgc gccggaagct ccatttttgc
     7141 agcgcggaca aaacacgctc ggccaggccg ccagcacgca gccagcctgc ggccacggct
     7201 tcgtcgggat tcaaatccat cacccacggc acaaccgctc cgcctttcag cgccgcaatg
     7261 actgtgccga gtgtggaaat cagcggcggc gaggtcaggc aaaccgtcac atcaaagcgc
     7321 ggcagccgca tcaggatgag agtggcattc atccagaaaa tggcgaagtc ggtgaagcgc
     7381 cgccatttgg ccttcttgcc caggcccggc gtccagatgc ggtggatctc aatgccctgc
     7441 caggtctccc gcaccgggaa gcggcgcgcc ggatcgtcat acccgcgcga ggccgccacc
     7501 actgtcacct cgtgcccggc ggcgcgcagg cccagcgcca gatcggtcaa atgctgcgcc
     7561 gtggacacat ggtccgggtg gaagcattga ttgagcagga gcaccttcat ggacggagga
     7621 cttctcagag cgaggccctg ccggctgcaa tctcgtcgga aatccaggca taggtcttct
     7681 ccatgccgtc gcgcaggcgg gtggagggct cccagccgaa gacttccttg atcagcgtgt
     7741 tgtcgctgct gcggccgttc acgcccttgg gcgcgtcgag cttgtagctg cgcttgagtt
     7801 tcactccggc gatctcctcc acgatgtcca cgagctggtt gatgctcacc atctcggcgc
     7861 tgccgatgtt gacgggctcg atgaagtcgc tttgtgtcag catttgagtg ccttgcacgc
     7921 agtcggtgat gtacatgaag ctgcgcgtct gctcgcccgt gccccagatg tctatttcat
     7981 gcgcgccgga gcgcttcgcc agggccacct ttctgcacac ggcggcgggg gctttttcac
     8041 gcccgccatc ccaggtgccg tgcgggccat agacattgtg ataacgggcc acgcgcgttt
     8101 gcagtccgaa gtcctcgcgg aagtgccggc acatgcgctc gctgaagagc ttctcccagc
     8161 cgtagccgtc ctcgggcatg gcggggtagg cgtcggcctc cttgagcgcg ggcacatcgt
     8221 agctcttctg cttgtctgca ttgtacacgc aggcggagga ggcgtaaaag tagcgcgcca
     8281 cgcccgcgtc acgcgccgcc atgcagaggt gcgtgttgat cagcacgctg agcatgcaga
     8341 gcgccttgtt gttttcaatg aagcccatgc cgcccatgtc cgcagccagg ttgtagagcg
     8401 tgtgcgtgtc cttggcggct gtctcgcagg ctgattttcc cgacaaatcg agacggagat
     8461 tctccacgtc atcaaagcgc tgataccact cgtcgagggg tttttgatcc acagcacgca
     8521 gtcgtacgaa tccctgcgcg cgaaggtccg ccaccagggc gccgccgatg aaaccgcccg
     8581 cgccgcagac ggtgatgagt gggtcatttt ccatgaatgc ttgaataccg gcccatgaaa
     8641 cgaaaaaacc cgctctgttg caagcgggtc tgtcgtctta tttccagcca ttggcgcgcc
     8701 gccgccggat gcgccacaca tccagcgcca tgcgccacga gtcgcgcagc aggctcacct
     8761 tgcctccggg cgtctcccgc cagtcaatcg gtgcctccgc caccgggcag ccggcgtcga
     8821 gcaacgcggc catcagctcg acatcgaagg caaagccatg cacttgcagg tgacccgccg
     8881 ccttttcata agccgcgcgc ggcaccagct tcagtccgca ttgcgagtca tagaccggga
     8941 tgcgcagcag ctcggacacc agcgtggcat acacgcgccc cagcaggtgc cgcttgaaca
     9001 ggcgctccac ccgccgcccg agcatcttca cccgcgaggc aaacagcgcg gaagggctgg
     9061 cgcagtccgt catggccatt ctggcaagac gcgcagcttc aagcgcgccg cacgagccgt
     9121 ccgcatccac aaatcccagc cactcagcgc cctcatgcgc cgcccatccc gcatacacgg
     9181 caccgccttt gccgaggttc tccggcaatt ccatgagcgg caaaaggcag gcatggcgtc
     9241 cgcgcaaatc ctccacgacc tttcgcgtcg cagccgcctc ggaatctccc gatccgtcat
     9301 ccaccaccag caccgacact ccggcgattt catccatctc cgcgcagagc tgaggcagga
     9361 accgcggcag ccgcgcgctt tcgcggaagc aggggaccac gagatgcaga cgccttttca
     9421 tgctatgtcc ggcatccgcc atgtcagcgc gcgcaggcgg attggtgttt gaacagcgcg
     9481 ttcatgaacg ccacccggcc gccatgctgg tgcatgatgt cgaacaggct gtacaactca
     9541 aagccgagcg gcctcaaata atcagtgaac tcatgcaatg agaccagccc attgtcctgg
     9601 tagggcgcgg gcaccgtctc aaggactatg ctccagactc tgcccgcgcg cagcatggcc
     9661 tcggccccgc gcaggatgtg caggtcccgg ccctcggtgt caattttcag aagatgcacg
     9721 acctccatat tgtgccgcgc gcaaaattcc ggcagcgtca ccacgtcaaa ccaggccact
     9781 cccggtcctt ccgcatcagc ggccgcctca tggttcaggg aattcatggt gaactcggga
     9841 tcatccgaaa ggagcctcac cttgaccctt cccggcgtgt cgctcatcgc cagttgatgg
     9901 caccggatgt ccatgccggc agtatgggat tgcagcgccc ggaaagaagc gggcacgggt
     9961 tcaaagcagt gaatctcagc gcccggaaaa tgctcgcgaa aacccgccgc cgtctgtccg
    10021 acgttcgccc ccacatcaaa aatgaccgga cgcgccacgc tttccaattc gggataacaa
    10081 accctgtccc agaagaaccg gtagccgtga tagtggcagt atcggaaatt caagcgccgc
    10141 gccagccaga gtttgagctt gagtttgttt aacgggttga ggtcgaattt cataagttca
    10201 atttccggcg aaaagcatct gccaggtcat ttgttgagcc ttttctccag cagccgcagc
    10261 gccagcagca tctccgccag catccgctgc agcgtgtaat accagcctcg ccagccatcg
    10321 agaatgacac cgcgcaccag cagcgtgtga accgccaccg caggcaccgc cgcccagccg
    10381 gtgaggcgca ggcggtcttg aagacgcagg gctgccgggt ccgcgcgcag gagtttttcg
    10441 gcctccagca gcgagtattt gtcctgtgag cttagccagc gtgtgagggg cttgcggtca
    10501 tcgtggtcaa tgagagtgct cagcctgcct gtcccgcccg tcacatgaag cagttgcgtg
    10561 tggccatcct gcgcgtagcg gcagcggtcc ttgcggaaaa gcaccgcgcg cggcggatag
    10621 agcgagccgc gcaaagcccg gccaaaggtc aaataccgaa agccggcctc atacgcgtca
    10681 gcctggtcat ccagagcggc cagctcattc tcaaaatccg gcccgatcac atagtccgcg
    10741 tcgagcgcca gcacccacgg cgttgtcaca gcgtccacgc cgaagttcca ttgcgaggtg
    10801 tgatcatcga aggtgcgcgt aagcagacgc gcgcctgggt gggcacacac catctcgcat
    10861 gtggcgtccg tgctgccgct gtccactacc acgatctccc gcgcccagcc caggcgcgcc
    10921 agcacccgcg cgaggtttgg ctcctcgttc caggtcagga tcagcggggt gacgagttcg
    10981 tgtctcatgc ttcctggtaa accctgtcag ctagcatcac cagccggcgg taaaaggtta
    11041 gcagattttc cagggatagc tcctggagtg gtgaggaatc tccgtgcccg gtgaagctct
    11101 cgagaactgt atcgtgactg cggtctgtca ctctgagacc tagcttttca aaaacctttg
    11161 ccacagttct tgtgaagccg cgcagagcta gttgggcgtc atccaatggc ccctgtcctc
    11221 cagtcatcgc cacaaagaca tcggggaaga aacgtgtttc ggtggaatag ttctggaagt
    11281 ggtggcgaat aatgcagtgt cgttggtggc cgcctgagcg gatgagaata tcgcatggga
    11341 tgccgtcgaa agtcatgctt gcggaaagag tctcgagttc gccacggtcg ttcctcgtta
    11401 cgaggcagcg gttcacctgc atttcgccct ggcaaaacat ccagcagaga tccaggtagt
    11461 gaatggcata atctgtgagc acaatccggt tgcgccactc ttgcttcatc cagctagagg
    11521 cttcgttcgc cggagacggg gtttcgaaat gcaggctgac ggcacgtaga gcaccgggcg
    11581 gatggttccg aagatgcgcg cgcatgcgca tgaggtttgg cttgaaacga tagttgtgca
    11641 gcacaaaaac atcgcgtgat ttgatgtatt ccatcatccg ctccaacatc gccgcgcttg
    11701 tcgcaaaggg tttttccagc agaacttggc tgacggtttc cgccaacccg tcaagaatgt
    11761 ttgggcgggc attgactgga gccgtgatga tcgcatgaga tgcgtcttgc gggattccgg
    11821 gatacgtctc gattttaata cctgcgcatg cgggcgcgcg tgtcagcgcc ggatcagacc
    11881 agaagatacc acctttaaaa cctaaacgag ccaggccagg caagtgtttc tggcccacaa
    11941 tgcgtcccat gccgaagtaa gcaaggaagc cgggcttgga gcgtgctggc aggggtattg
    12001 tcggaggcca cggcggtgaa ggtttcagat gaacaagatt acgcaaagtc tccctccagt
    12061 cgaaactcat ttccaggtca agatcacgag aggttcgtgt gcagtcataa gtccgcacat
    12121 tgagattgtg cataaagagg aggtccagac gaatgagacg gcgtttgatt agcgaaatcg
    12181 ggatcgaggc tgccgccaat gccaagcgcc acatccaggc cgggaaatgc agggtgctcc
    12241 atccgcgtcc gagttcgcgg cattgaaagt ccaggtattc ccttcgattt ggagcattcg
    12301 gtgccaccag catgtatttc ttcagtttcg gtgacgaact gtttgaagcc gaaccggcga
    12361 ttttcacgag cgcctggtcc acagcatctc gatgtacaag cataatgacc gtgcgttggt
    12421 tgccgagacc gagcacatgg ccggtgggca gttgcttccc agtgccgacc atcgaatcaa
    12481 ccaagcctgg agcaaggata aagcccggac gcaccacatc aagatctcct tctcgccatg
    12541 tgtcaatgat gtgtccttcc actgccgcct ttacacgcga gtagatgcct ttccatcgca
    12601 cctcgacggc ttccgcgtcc tcgttgagca cccctgtaat ggagggcagc acgctgattg
    12661 aggaaatgtg aatgaaacgg cggacatgca acttcttcgc agcttgcatc acctgatctg
    12721 taaggctgat gttttcctca atgctgccat ttttgactat ggcgaagttg atgagcacat
    12781 cgcagacgcc atccatggcc gtgccagtga tctcagccag tcggtcgtag catccgtgca
    12841 ggtctctcgg gccagagttc tcgatttgcg agcggttaaa ggtccagact tcatgtccgg
    12901 ctgcaagcag agctgccgga aggttcctgg ccgcgaaact ggatgaggct aatactagga
    12961 ttttcatgga gcagaaagat gggcgaggca ggcagcggtg agccgttggg cggcggactc
    13021 ccagctaaag tgttcagcag ctgtcactgt ggctctggat gagatttcct tcctctcggt
    13081 gcttgatgcc agaagatcct tcatggcttc tgccaacgca tctgccgaca gtggcgtgac
    13141 gatgcccaaa tgatgttttt tcacctcgtc ctgaagtgcc acttggtcgg agatgacgac
    13201 aggaagcccg caggcgaccg cctccaaaac tgcgatgccg aagttttccg aataggatgg
    13261 cagcacaaac atatccgcac cctgcagcac gagccacttc tcttcgccct ctttgaatcc
    13321 gagccaatga acgcgccctg gcaatgactg ctcggtggcc tgctttaaac ggttcagata
    13381 cgcctcatct ccggtgccga cgattgccag atcaaagcag gcgggcagct ttgccgctgc
    13441 ctccaaaagc atttcgatgc ctttttttgg atgaatccgg gacatgaacg tcacaacatg
    13501 cctgtctgca ggtaggcgga gcagcttgcg cagtctttcc cgcgcatcaa ccagacgtgg
    13561 cggcatcacg atgccatgag gcagcaccaa gcaagggctg ctaaaaccct ctgaggccgt
    13621 ttcacgcgcc tccatttcgg atgtgcaatg aatgaagcgt gctccattga tgtttcgccg
    13681 ggtcaccaag gccagttgga gttgcttgag caaccgccgc tgcaggagtg accaatgaca
    13741 gagctgcccc agtggcctga cgcagtatgg cacgcctgcg ttgcggcaga ctcgcatcgc
    13801 agaatttgcc gggtgggaga acacggtgtg aatatgagcg aaatcgtatt cacgaacatg
    13861 cgcgcgcaac caagcgggat actctggcgt gaaagtgaag cctgccagcg tgtgctgcct
    13921 cgcccttacc cgaggaagaa aaaaaacagg gacgccttcg tgttcgatcc atcgccggag
    13981 cggaacattc aagcggccgc ccaggtcgtc atcactgctg aggatgaccg cgtccacccc
    14041 caactccctt tgggcacgga ccatgctaaa cacggccact gtgggccctc cgtggcgggg
    14101 ggccaatgat gccacacagt gcaggatttt catcagaaga gattctttgt cgcaatcagg
    14161 tgcgagcgtg agatgaaata tatccggtca aacccggcgt ttgtgagggc tttgcgcact
    14221 tttgctccgg cctgatgttc gccattggca gccaggatgg catagtgaat ctcgcaaagc
    14281 acggcgcggc attccgcgcg tgcgagttgt ttttccaaac ccttgagggc cttcagttcg
    14341 tggccttcaa tgtctatttt cagcatcgtg ggcggaaggc actcgccaga tgccacaagg
    14401 ccatctccag tagccgctcg cacttccatg caagacatgc ctgtcgtgtt ttcaatggag
    14461 cttgttgtgt cgctggcggg atcaaaggag atgggcacca taccctcatg atcgctcagc
    14521 gccagattga gtgcggtgac attgcatgca ccagcggcac ttaggttggc gcacatgccg
    14581 tgaaatgttg ctggaacagg ttcaaaggca tacacgctcc cttcatgtcc cacgagttgc
    14641 gaaagcaggg tgctgtaaaa gcccgcgttt ccgcccacat cccatgcgac gccatttttc
    14701 agcactggtg cgaatacatt gacgcaagcg acgagtgttg gcagttcatg ccggctaagc
    14761 atcatgtccg cgtagtggcg tggaggcccg gctcgaatga cggtgcctga tgctgaaaca
    14821 atctccgagg tttcagggga catggaccgt tgaacgatct tgccatgccg ggccagaacg
    14881 aaattttcca gccttgtcat gagtggagaa aggcgtccgg ccatacctgt ggcacgcagc
    14941 agagatcgaa gatgctcaag aatcatgaag tgaaatggct ttgccggtta agtatgcgaa
    15001 tgggctgatt ccagccactg ccgcagcatg aagacgcacc attgctggta ccaaggagcg
    15061 cgcgggtcgc ggatgtccgc gcgagcttgt tcgaaggctc cgccccagtc ggcggccagc
    15121 catttttcat acggaaacaa aaaaccgcgc ttcggctggc cggccaccca gtctggaatc
    15181 tcgggcacgg catcgagcag gagctgcttg cccgcgcgca ggcggagtgc cgcagggatg
    15241 tgcgccacgc tttcaaaaag cgcgcggtcc accagaggca cgcgcaactc caacccgtgc
    15301 gccatgctca tgacatcact gtctttgagc agttgattgc gcatgtagag tgtcatctcc
    15361 aagaggctcg cctggtcgcg ctcatcgggt gtgtccgctt caagcgcagt ctcgggtgag
    15421 agggaagctt cgtcgcagcc cgagtagaga gcggtcaaac ggcgcgcttg tgcgcgcgtg
    15481 aaaataccgc gaaacacgcg ccatgcctcg gtcatgccgg gaccgcgctg gagcaggtct
    15541 cccacacgcc gcaggttcgc gcggggaagg gtagtctcca tccagcggcc catcatcgtg
    15601 gagcctggca gcttgcgcag ccagccggcg gcatgagcga gccggggcac tttttcaaag
    15661 ctggcgtaac cgccgaaaat ctcatcccct cccagacccg agagcaccac tttcatgccc
    15721 tgcgcgcggg caaagccgga caccatgaaa gtgttgaagc cgtcaatgcc aggctggtcc
    15781 atgtgtctca aaaagcgcgg gaacgactcc gccatgcctg ccgcatccag ccgcagctca
    15841 tggtgacgca ggcccaggtg cgcggcggtg cgcgccgcca cgctggactc gtcaagccgc
    15901 gcgtcatcca cgccgatgga aaagctctcc agccccgcgc gcccttgctc gcgggctagt
    15961 acagccagca gagtcgagtc cacaccaccg ctcagaaaca ggcccacagg cacatcgctg
    16021 acgaagtgat gatcgagcga gtcgagaagg gcggcgcgtg ttttttccgc agcctccccg
    16081 gcgctcatcg gcccggggct gaataccgga cgccaatggc actccatgcg cggcgcgccc
    16141 gccttccaga tcatggaatg tcccgccggc agcgcgcgca cttctttgag cagggtcgca
    16201 ggctcggcca cgctgccgct ttccaggtag cgcatcatgg cggcggcatc cagcgagctg
    16261 tcagccagcc cggccgactg aagcgcgcgc acctccgagg caaaggcgag gcggcccgcc
    16321 tgatgcgagt agtagagcgg cttgatgccg aaggggtcgc gcgccaggaa ggccgtccgc
    16381 tccgcctcgt cccagatggc caaggcaaac atgccgcgca gcctgcgcac cattggcgcg
    16441 ccctcgcgct catacagccg cagcagtacc tcggtgtccg agcgcgtgcg gaactccgcg
    16501 cccgtttttt caagctccgc gcgcagctcg cggaagttgt agatctcccc gttgaagacg
    16561 atggcgaacc tgccatccgc cgtaatcatc ggctgatgcc cggcggggga aaggtccagc
    16621 acagccaggc gcgtgtgagc caggccggcc agaccggaag gggaaagcca ttgcccttcg
    16681 tcatcgggac cgcgatgcag caacgccgcg cgcagcttct ccaatgccgc ctcaatgggc
    16741 gcgggctcgc tctgaaaaat gccggcgatt ccacacatgc gcgttcaatc ccaaatctcg
    16801 tcgaggatgg ctttcaatcg tccgcgaaag gcctccgggc cgaattgccg caccacatct
    16861 tcgtgcagtt ttgcgggttc aaaaagcagc cggttgggat gtgtccccgc cagcagactg
    16921 cgcaaggctt ccgtcaattc ctcgcggctt tgcgggttca ccagcactcc cagcctgcct
    16981 tgatccaccg catccgccga gccgtcagca ttgcccgcca ccacgggttt tccgcaggcc
    17041 agtgcctcca gaaagacgat gccgaaaccc tcgccccggc ttggcatcac aaacacatca
    17101 cacaaccggt aataatcggc cagctcagtg ttggacacga atccggcaaa gatcaccgct
    17161 tcctccggcg cgtgcttttt gaccaggcgc ttcaactcag cggcgtagcc tccgcggccc
    17221 gcgatgacat agcgcaggcc ggggaactcc tttagcaact ccggcaggca ttcgatgacc
    17281 tcctggtgtc ctttgtaacg ctcggactcc tccagtcgcg acaccgtcag cagcacaggt
    17341 tgtcctgctt ttaatccgtg gcgttccagt aacgcctcag gtttgggacc gggctggaaa
    17401 gcgccggttt gaaatgtgtt gggcagcaca aacatgcgcg ctccttccat cccaggaagc
    17461 tttgccacct gatcgcgcgt gaagcggctg accgagatca ccgcgtccgt cttgcgcagg
    17521 gcgcgcagct tggcgcccgc gcgcagattc cacgcgtcaa tgccgtgcag caccactgcg
    17581 gtccggcagg cgccgggttt gagaagatgc acggcggaca ggaaattcac atgcgtcgag
    17641 agcaccaggc agggacgcat gagcagcgtg cgcaggatgc cggacaacgc gaaatgcagt
    17701 gtgcgcaaaa cacggtgaaa tgcacgcgag gaagaccagg tgaccctttc cgcaaaaagc
    17761 gggtcgtcct cgcccggcgc gtcatttttc acatgcacat gcagccgcgc ctcgggatag
    17821 gccgccagca aagcgcgcac cgcgtcgcga ctgaaagcct ggatgccgcc gctgccctgg
    17881 cggatgccgg gagcccagac atgaatcaag cgcgtgctca tgacgcagga ccggcatcct
    17941 gcccctcccc tgtggaccgg gtgatcctcc tggctgctcc atccacaaac ccatgcggta
    18001 gcgaattcgc atcctgcaaa tcttgatggg cgcgccgcag ccgcagccag gaaagcagcg
    18061 cccgcgcgga cactcgcccc gtgtgtccgc cataagccag cagatgcgtc ggcgtcattt
    18121 gcaggccgct ccacactgcg cccgtctggc catgcgcgag catccagcgg atcttggaca
    18181 atacctgcgc cactcctgcc ggccagagcc agaccggata acgcagccag ggcagcagca
    18241 ccgtgttcgc cagcaccacc gccgccaccc tttgctcgtc acgcgcgcgg tcggcaggcg
    18301 gctcatgatt cacccgcagc aggggatcat gaatcattcg gccgccgagt gcgtggagtt
    18361 gcagactcat gtccacttcc tccattccat aagcgatcgg cagcggcacg aagccgcgtg
    18421 tgcgcgcaaa ccagcgtttg ttgaagacac agccgcatcc ggaaaacacg gagatgcgct
    18481 ggagcaccag ccgcgcccat tccagcgcat ggctcgccgc gctgagcacc gccgcgtcgg
    18541 gaaaagcgcg cgccagctcc gctgctcgtg cgaagtagtc ccgatgcgcc gggtatgagt
    18601 cgtcgtcaaa gttcgccacc cagtcgtgag cggcctcggc gatgaggcgg ttgcgcgagc
    18661 cgccagggcc gagacgtttt tcggaaagca ggcagcgcac tcccggaaac cgggcctcga
    18721 tctccgagta caacgtggca ttgccgccgt ccacatgcac caggatctcc gccggggccg
    18781 ggtcacaggc cagcagccgc tccagcgtgc ggctgaccat gtccagccgc tcatgcgtcg
    18841 gcacgatggc ggtgatctgc gtcatttcat acgagccatg tcagccatgc gcgggctgtc
    18901 ttgacggcgt ccccgtcctt tgcggcctgc ggtggcgaag catcacgagc ggctccagag
    18961 tcggcagcat ggccagtccg cagaaggcaa aaagcagaat actcgcggtg tggttcatga
    19021 ccacctgcat gctcagataa ggcagcgaga gcagaaaggc cgcaagggcg aaggacttct
    19081 gaacaggtgt ccgcgccagc gtgtagcttc gccagcacaa ccagaccaga agaaaacgaa
    19141 acgcatacca ggccacgaaa ccaaacgcgc caacatccat ccatacattg cccgcctcgg
    19201 cctccacgcc cacaggctgc ttcgccggac cggcgaggcc caggaaattt ttcagacttg
    19261 ccgtcgcagg atgagtcaag cccagtccat agccaaacca gcccgccttc tcaatggcga
    19321 aggacatcgc ctcgtgagtg tggccgatgg tgcgctcata gatcgtgtcc gccgccgtgc
    19381 tcgtgcgcac cgacatgtaa aagaaggcct cgtaaaacca gtagctgatg atgagtcccc
    19441 ccgccgtgat ggaggtgatg agaatggtgc gtatcggcgt gccctggccg attctcccgg
    19501 cgaaggccgg caaggccatg cccgcgatga tgaaagccgc cgtgtagagt ccggagcgcg
    19561 agccgctcat caggctgttc accgccagca agggaaggat cgcccaggcg atgacccatt
    19621 tccaccttgc ctcccgcagt gtcagcatca ccaggcagag agtcacaaaa aacacgatga
    19681 aagtcgcgtg acccgtgatg taagagaagg tgcccgtgat gcgcgcgcga tcgcccatgc
    19741 ctgctccaaa gccgtcgttg gtgattcgat caccgtctct ttgcgcgtac acattcagga
    19801 tgctgtccgg ccccgcgcgc cactgggcca ggccgagcag gcagattggc agggcgatca
    19861 gggtgaacca tgtcatctgc ttgatgagtt cctcttctgt tcggaagagg aagggcatca
    19921 tgaaaagcag cggcagataa taaaagtagg ctttcaggcc gaagacgctg atgatcgccg
    19981 aattgatgtt cgggttcagc gcgaacgcgg ccacgatgac gcacagtgcg aggatcaagc
    20041 cggtgggaat gcgcagccgg tgggaccgca catcgagatc aggtgccaga aagaatttca
    20101 ggtaggcgcc gaagaggaac acatccttga ggaaatacac cagctcctgt ccctgcggga
    20161 aggcccactt gcgcagcgct ccttcaagca gcgccaccac cagagccgcc ttcactgccg
    20221 tgcgccaatg ataaaaactc catatcgcca ccaccagcgc gccccccgcg ccgactccca
    20281 gcagggttgt cttcgacaag ctcgcggcga gaaaaatgtc aggggtagtc atgggcggat
    20341 caggcttgga cggatgaagc ggaagcctcc gggtgcaacg cgatcattcg gagattgcat
    20401 ccgcgctcct gtgctgactc caggcttaac gcctgctccc gcacatccat ccaggcggtg
    20461 tatccggatt ctttcagcag ctgcagcacc tcgccgggtt ccgatccgcg cgcgcggatc
    20521 aaatcgccgt gcagttccag gaaaatcaaa ggcttcgacg cggccagtgt ctcacgcgcg
    20581 ccgcgcagca cttccatttc atagccttca acatcaattt ttaaatgcgt cggagcgaac
    20641 ccgtgactca gcgccatttg atccaccgtg acctgctcca ccgtcacgca atccgggcgc
    20701 gtttcctggg gtaccacaaa ataatccgcg cctcctgccc ctgtggtcag catggaaagt
    20761 gtgcccggct tgtcaccgca agccgcgttt accagcgtta ctctgccgga agcgttgttg
    20821 agcgccagat tgtccttcag cacgcgcgcc gccgcgtcgg aggcctcgat gtccaggcag
    20881 cgcgcctccg gtcctccata atgcagcgcc gccagcgtga acatgcccca atgcgtgccc
    20941 acatccatga agcgcatgcc tgatttgcag agccggatga aggacagcaa ctcctgccgc
    21001 tgctcggcgt catgctcgaa ggccaggaac tcctccacgc acatcgggtg gcagcgcaga
    21061 caccagccag acgccagttc cagcccgccg ctgccgcctg caaagcggcg cagcttgctc
    21121 aatacgcgcc gcagactcgc ccccggtctc ctcaatgagt tgcgcagacc tcggggcacc
    21181 cagcggcgga tccattcttg ggcgttcatg aggtggtgtg cggggttgtc atctccagca
    21241 gcattcgttc aaagcttgcc acaatgcgcg cccgttgatg tctttgcaga tgctctctgg
    21301 catcgggcgc gccctgctcc aaggcggttt tcaagcagcg tgccaaattc tcgacattgc
    21361 cggattcgaa gatgatcccg cacggccccg ccgcctccgg caggccgcct ccatccgacg
    21421 ccaccaccac gcagccgctg gccgcgccct ccagggccac gatgccgaac ggctcagccc
    21481 agcgcgaggg cactaccaac acccggtgcg cgttcatttc ccgcgccagc gcctcgccac
    21541 gcagcgggcc ggtgaagcgc acccggtcct ggatgcccag cttttccgcc tgtgacttca
    21601 aagccggctc ctcagggccg gagccggtga tcgtcgctga aacgtgcatg tgctgcgctg
    21661 ccaaacgggc cactgcttcg agcagaagat ccgcgccttt gtccgacacg agacgcccga
    21721 cgaaaagcag gtcgcggtcc ctcgccactt cggggcgcgg gtgaaaaagc gcgtcgtcat
    21781 acgggttcgg gatcacgatg tgcggcaggc cgagatcacg cccgatggca gcgctgcatg
    21841 tcacattcac ggcaaggcgg caggccagtc gtttcagccg ggtctgccag gtgagcggcg
    21901 cgccgccttc ctcgcgcagc caggtttgat gactcaccag cagcggacgg cgtgtgatga
    21961 gccagggtag caagcccttc aaactgatct gcacatggat gacgacatcc gcccaggcca
    22021 tccagcgcgc cattgcctgg aagcctgcgc ggcgcacgac gcggaagggg aagggctccg
    22081 cctctccagc gggcaggtcg gactggcaga gcacgaccac ctcatgccct ctctcatgca
    22141 ggccacccgc cagcatcgct gcaaatgcct ccagcccgcc gatcatcgga tggaaggcgg
    22201 gcgaatggat gacgactttc atgcggttgc caccctcaca tattcacgcg ctgtcatctc
    22261 cagcgagtgc ggggcatacc actcgcgcag tctgttctgc acgccttcga gatcatgagt
    22321 ggatttcaaa tcagcagtgg ataaaaaatg tcgcccctcc gtaagccctt ccgagatcac
    22381 accatgcccc tgcgtcagca caggcaccac gccatgcgcc gcataggccg cgagcacgcc
    22441 ggacttgccg agatagtccg ggttgtaatc caggatgccg cagcgtgacg agcgcagttc
    22501 ctccgccatc gccgcaggct ccagcacacc agtttgcctc acgacaaagc gcgcgcggat
    22561 gctttctggc acagtgattg gatgactcac catcaccagt tcggaaaggc tgagctggtt
    22621 catggcggcc tccacctgaa gccagaaatc cggccttcga gccgggccgc cgccgaacgc
    22681 catcagacgc gccgggcgcg ccatgggcag cttgggcgcg gcatcctcgc cgaaattgga
    22741 gaacaccggc agcgcgctga catccgcgtt ttttgttagg tgcttgcgca gccacacggc
    22801 ggaatcacgg cggttcgtca cagccgcatc actcagccgg gccagtcccg ccgccgcatg
    22861 ccgctgcagc atggaaaacc aaaacgcgct cgtccacggc gcgccgcccg cgaagagctc
    22921 gtgaaacatc gtcaccagac gcgtctgcgg acgctcgcgg cgcatctccc gcatggaccg
    22981 cagcaggccc agcggcgcgc cgcgtttcgc atagccatag cccgagtagt gcaggaccca
    23041 aatcatgtta tcggtgcttt ctcgggaagc cgcgagcatg acttcccgcc agtccgcctc
    23101 agatttgaaa ggcaccaaag ccgagcccat cccgcgcgtc tggaatgccc ccgaaaggca
    23161 gcgcgcataa tccgccacac cgcacacggg tggcggctgt cgcgggatga gatggagcca
    23221 gcgcatggtg attgaagtgg aagcagcgtc ccactgctta cgcatggagt caaaaccgag
    23281 ggcactcatc cagcacacat tgccctcacg ctccgcctcc ctccagcaag cgccggaaaa
    23341 acctgcccgc ctcccgccgg taacgttccg gggtgaactg cgccgcaaac tcctgcccgc
    23401 gccgcccctc caccataacg cgttccgggt tctccacata agccgcgatt tttgacgcca
    23461 agtcttccgc atccatcgga cgcacacgcc agccggcatc cggcgcgggt gcgccgcagt
    23521 tttcactgat caggaccggc gtgccgcagg ccatcgcctc gagcgccacg aaaccgaagg
    23581 aatccgccac cgaggggagc acaaaaagat cgtgctccgc atagagcctg cgcagttccg
    23641 ccttcgtctg cggcggcagc agacgcactc ggtcttgata agacgcgaac ctttccttca
    23701 aagcctcatc cacccgcccc acgagtgtaa gctcccaaga accaccgcca agagcgaagg
    23761 cctccagcaa gaatgggatg cctttgcgca gcgtgatgga acctgcgaaa agcaccttca
    23821 atctgccgtc gggctcacga tgttcctttt ccggaaacca aagcgacggg tccacccaca
    23881 gcgggatctc cacttgctgc cccgtgggaa agccagcccg ctcaaagctg cgcctatgca
    23941 tggaagaata aaccatgcgc cagtcggcca gttcgtattc tcgctgcttg cgcgttgtca
    24001 tggcgggatc atcccgttcc acagcagcag gcagacgcgc acgatcgcca gcttcgcgca
    24061 tgacttccgt cagcaggccg gggtgcagtt gcggacagtc aagaaccttc accgcgccga
    24121 tttcttccgc tgcctcaaag ctgtgccaag cgcaagtttc cgtaccgacc aagatacgcg
    24181 ccggattctg agcgaaccgc cttgccgccc atcggtcgaa ggcatcattc accgccagcc
    24241 agtccgccgc gcgcgcggga accagc
//
LOCUS       DAOMYJ010000057        24245 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Prosthecobacter sp. isolate AMR_MDS_5629
            NODE_750_length_24245_cov_22.234560, whole genome shotgun sequence.
ACCESSION   DAOMYJ010000057 DAOMYJ010000000
VERSION     DAOMYJ010000057.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867850
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prosthecobacter sp. (wastewater metagenome)
  ORGANISM  Prosthecobacter sp.
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Verrucomicrobiaceae; Prosthecobacter.
REFERENCE   1  (bases 1 to 24245)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 24245)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.30
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:34:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,742
            CDSs (total)                      :: 4,684
            Genes (coding)                    :: 4,676
            CDSs (with protein)               :: 4,676
            Genes (RNA)                       :: 58
            tRNAs                             :: 55
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24245
                     /organism="Prosthecobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_750_length_24245_cov_22.234560"
                     /isolate="AMR_MDS_5629"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1965333"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..851
                     /locus_tag="PLP58_08115"
     CDS             <1..851
                     /locus_tag="PLP58_08115"
                     /inference="COORDINATES: protein motif:HMM:NF012437.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HRJ08516.1"
                     /translation="GIGAGALALAWLLGGVFSRQLSRPIHTLVAATQAVRGGDFETRL
                     PEAGTDEFAELNLSFNEMTEGLALKERYHSVLSMVTDARVAEQMMAGKIKLGGEAREA
                     SIMFCDIRGYTAMSAGRDPAEVIAILNRHMGALTEVVYRWHGVITQFAGDAIMAVFGA
                     PTHHGEDAVNAARCALEMLAARARLNEGSALPLGIGIGIATGNVVAGCIGAEKRADYT
                     VVGERVNLAARLCGAAAAGQVVIDETTRARLPGDVVTEALPEPLKLKGFTDPVPASRV
                     LRLNSQ"
     gene            854..2062
                     /locus_tag="PLP58_08120"
     CDS             854..2062
                     /locus_tag="PLP58_08120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ08517.1"
                     /translation="MRFLAITAALFAMQAGLCRAQAFLETLDEALSLHSTDAFWRADV
                     SGLLDLETYVPETPALGLLNTDDEVFFNPRLSLFLDLRAGETLRAHAQMRVDRGFDPG
                     FAPDGQTRLDEYFIEWRPLGDDALAFRAGKSATVFGAWTRRHFSWDNPFVTAPAAYSD
                     MLPVVDHRAAPRGALLGRRGERDNPLTWLPVVWGPSYATGLSLFGQVSDFDYALEIKN
                     AALSSRPHTWDAAQNGWTSTTWTGRLGWKPAPEWAFGGSFSHGPYLLEDARRLPRGRG
                     FEDYAQTTFALDAAWAHGPWQVWGELMTSRFAIPNAGGARVLTGFIEAKRKLGSGSWL
                     AARWNQSWFGDVPGTSTAWDNDGWRADLALGHRLSRHLQAKLQYSLAGRSGPHDEGEH
                     LLAAQVTVRF"
     gene            2126..3325
                     /locus_tag="PLP58_08125"
     CDS             2126..3325
                     /locus_tag="PLP58_08125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009959287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin domain-containing protein"
                     /protein_id="HRJ08518.1"
                     /translation="MKPLLVSFLLPALALAQPAPKAKKAASSMPEGFHELALGDAAPD
                     FALPGIDEKTHTLKDYAGARLLMVAFISNHCPSSQAIEARLKKFTAAYEGKGVQVVAI
                     NPNDPAALRPDELGYSKYNDSFPEMKRHAAEQGFNFPYLYDGETQKTALAYGCLATPH
                     VFLFDQERKLRYKGRFDDSRFADEASVTSHDARNAVDDLLAGRPVALAETRPHGCSTK
                     WITKREAVAADQTKWETTPVDVDLIDAKGVAALRKNDTKKVRLFNVFATWCGPCVEEF
                     PELVNTQRKFALRDFELICISINEPDEMQEVKEFLEEQNCIVPDKLKPSLKAEGRKGN
                     AYVFQGANQDELIAALDPEWPGPIPHTLVVAPGGEVIFRHNGEVDGDELRAKILEHMG
                     RYFTPED"
     gene            3337..3972
                     /locus_tag="PLP58_08130"
     CDS             3337..3972
                     /locus_tag="PLP58_08130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ08519.1"
                     /translation="MKKLLLSLLMPCALHAQEARSPNELFREGVALFFEAKPRESVAA
                     FDKLLELEPGAKPKLWQRGLALYYAEDYQGGREQFEVHQTVNTADVENAAWHFICVAK
                     KEGVEEARKHLIPISGDSRVPMKQVHDLFASKATVDDVLAAAEQGEGEDLRNHRCYAH
                     LYLGLYFEALGQEAKAKEHILKAAVDFSMDHYMGRCSQVHARVRGWDAKKE"
     gene            complement(4014..4427)
                     /locus_tag="PLP58_08135"
     CDS             complement(4014..4427)
                     /locus_tag="PLP58_08135"
                     /inference="COORDINATES: protein motif:HMM:NF019262.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ08520.1"
                     /translation="MKIQTALAALIVSTSLAAAEDISFEKQILPVLKTSCFKCHETEH
                     EEDGRVKRPKGGLVLDTAEGIMAGGKEYPKENVVAGKPDDSWLLKTMALPESDEYAMP
                     PEGKGDRVSAADQELIKKWIAAGANFGGWTKAAKE"
     gene            complement(4549..5190)
                     /locus_tag="PLP58_08140"
     CDS             complement(4549..5190)
                     /locus_tag="PLP58_08140"
                     /inference="COORDINATES: protein motif:HMM:NF025167.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HupE/UreJ family protein"
                     /protein_id="HRJ08521.1"
                     /translation="MKSLTAVSALTMLLVLGVQELLALARGMLDLEVLGAGLASGFRH
                     IIPEGLDHIAFLLGLFFLSRAFPALLAQVTIFTLAHSLTLGLVVLTGFSFEPRWVEVA
                     VGLSIAAVAVEGMFSCSLARWRWLLVIAFGMIHGLAFAHNLVQEEGIRRSPVSALFGF
                     NLGVELGQLVVVGALMLVFSPWWRRPWYRVRVALPALACTALCGLFWAVQRAA"
     gene            5282..5977
                     /gene="radC"
                     /locus_tag="PLP58_08145"
     CDS             5282..5977
                     /gene="radC"
                     /locus_tag="PLP58_08145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009961495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HRJ08522.1"
                     /translation="MSHRIHDMPEDDRPRERLLRLGPGALSDAELLAIFINTGVKGEN
                     ALQVANRLLREVGTLHNLSRTSASQIAASHALGPAKAAHLAAAFELGRRAERFWVKDT
                     PLDEPEKVYRYLAAEMQSLPHETLRVLLLNARFHLMRDEVIFTGTVNETMAHPRDILR
                     PAVIHKAYGFTVVHNHPSGDPAPSTADRAFTRKIKDAAEVLSIKFCDHVIIGMPDQSG
                     RKPYFSFREMGAV"
     gene            6014..6631
                     /locus_tag="PLP58_08150"
     CDS             6014..6631
                     /locus_tag="PLP58_08150"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018971109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004049 - anthranilate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0046820 - 4-amino-4-deoxychorismate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate/anthranilate synthase
                     component II"
                     /protein_id="HRJ08523.1"
                     /translation="MGPPACFSTGAADGRPMLLIIDNYDSFTYNLVQYFGEMGARMQI
                     HRNDQITLEEIRRMNPDHICVSPGPCTPKEAGISCDVIRAFGGSTPILGVCLGHQSIG
                     HVFGGDVVRAGRLMHGKTSMIHHTGKSVFKDLPDPFEATRYHSLLVKRDTLPECLEIT
                     AVAGDDESEIMGLRHKELPIHGVQFHPESILTTEGKKLLRNFLEM"
     gene            complement(6635..8470)
                     /gene="rho"
                     /locus_tag="PLP58_08155"
     CDS             complement(6635..8470)
                     /gene="rho"
                     /locus_tag="PLP58_08155"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018969950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008186 - ATP-dependent activity, acting
                     on RNA [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HRJ08524.1"
                     /translation="MSEELLLDLPSSEPPVKKAAAKKTPAKKTPAKKAAAKKTAAKKA
                     AVKKAPAKKAAKTQAAVEVEVTAPEENPVAAQAETETPAEPKLPRKSVTVVKKAASIL
                     KKMQKAALNAAPAAKNPESPAIEIAPSEPEPPPAVEQPSVAPAAAPAMVETSAPVSIE
                     TSVPETAPAVENEAPDGEAAPPAQPERKKSKFERWKERRERFRQEKLARRLAAQQAAG
                     EAGADPDAGNGEDERSRTPEVVLGPPEPAEGVLELTPKGYGFLRRRELGYAQHPQDAF
                     ISGDFIRQYGLREGMAVKGIAQKGPRGPQITGVGEVNDRAPEAMRDLPLFEELKATNP
                     CKKLQLETTKDRLTTRVIDMFTPVGRGQRGLIVAPPRAGKTTMLQHIAEAVVANHPAI
                     HLMILLVDERPEEVTEFKRILPKAEIYASSNDQDVKSHTRIATLAIERAKRLVECGEH
                     VFMLMDSITRLARAFNNAKSGGATMSGGMGVGAMEIPRRLFAAARNTREAGSLTILAT
                     ALVETGSRMDDLIFQEFKGTGNMELVLDRKIAEQFYYPAVNIFKSGTRREELLLQSFQ
                     LDKIHMLRRGLAGHKPIDAIQRVLTLMERYPSNAQMLVDLPSKGG"
     gene            complement(8490..9239)
                     /locus_tag="PLP58_08160"
     CDS             complement(8490..9239)
                     /locus_tag="PLP58_08160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009962695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HRJ08525.1"
                     /translation="MAGHNKWSKVKHIKAVVDVKRGKVFSKLSKELTVAAKHGGSNPD
                     LNARLRSAILAARAANVPNDNIERAIKKGAGELGGDAIEEVTYEGYGPGGVAFMIEVV
                     TDNRNRAANDLRVLLSKNGGTFADAGSVAYQFTRRGEIRLPKGGLDEDGAMELALESG
                     AEDVEDADEEWLLYTATDQLFQVAGALRDKGHESTSQKLIYQAATPVILTDLDQAKAV
                     IKLHDQLDDYDDTQNVHSNFEISDEIADALS"
     gene            9689..10342
                     /locus_tag="PLP58_08165"
     CDS             9689..10342
                     /locus_tag="PLP58_08165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ08526.1"
                     /translation="MIELYQEIIAPHNLPVTLLLGAVFLYWLLVLAGILDFDLDILEI
                     GDATADLGDAADAHHGHLGGLWLSFGKALGFAKVPILIWGSFFSLFLWAGNVALNYYY
                     NGSPGERSMSTAALLFVPGVLGSLVVTKIVTLPIGRLFTAMAESATENVDVIGRTGVV
                     VSAEATDQFGQLQIGNEGAPVLLNVRLTPGGAPLTKGASAKIITASPDGKFYLIEPA"
     gene            10385..12460
                     /locus_tag="PLP58_08170"
     CDS             10385..12460
                     /locus_tag="PLP58_08170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ08527.1"
                     /translation="MHTPIAIIELLGLPVIGGIVGLIAGVIILISRFYRKVHQGQAIV
                     RNGIGGTKVSFNGMLVIPIAHHAEYMDISVKRVEIERRGPEGLICKDNMRADIKVAFF
                     VRVNKTNEDVLRVAQSIGCQRASSVDTMRTLFDAKFSEALKTVGKRFEFVQLYNERDH
                     FKNEILKVIGTDLNGFTLDDCAIDFLEQTQLENLDPQNILDSEGIKKITELTATQAKL
                     ANHIQRDKEKVIKQQDVQAREAILELERQLAETEAKQKRDVESVKAREEAETLKVQAE
                     ERLKAERAKIAADEEIAIANENMERQVLVAQRSKERTDAVEVERVTRDRDLEIIERER
                     VTTLKDIEKEKAVEVEKRNIQEVIKERVALEKTVVVEQQKIKDTEAFATADREKQVAL
                     TLAEKAAQEQLIQKIKEAEAAKEAARLAAEQDAFTTVKEADAAKEAAQLRAQEMLTLA
                     EAKQEAAAREAAAEKALAEAVAAKTAAPGLGEAQVLLAKAEAAQKQGHVDAEVLQLKL
                     AAEADGITKKAQAMKLLEDAGREHEEFKLTLDKEKSVELAQIHIQKDIAEAQAGVLGE
                     AMKSAKIEIVGGESQFFDKITSAIGNARAVDRLVEGSRTLSDVKETFFNGDPDYFKAQ
                     LKTLVDQFGLTAEDIKNLTLSGLFAKLIGLNPDAETLNKLTGMIGAATRFGLAEQSVG
                     ALLTGKAKK"
     gene            12520..17616
                     /locus_tag="PLP58_08175"
     CDS             12520..17616
                     /locus_tag="PLP58_08175"
                     /inference="COORDINATES: protein motif:HMM:NF023874.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair ATPase"
                     /protein_id="HRJ08528.1"
                     /translation="MAEPTPSPQAASPQLEAGAYEVIRQRLDKHGAELQRRLDLLNND
                     RKAEFGGIDTALLATTRLTTDNNCVPRDMTAIGPRRFLFGYNVHLGLRSQMHVEDVFA
                     GYDYNPEDHSFHPIKDGLLSDPQFVEDFAYLYGYYKNASFLKFHRIGPHLYMGMSVGR
                     GAAEVKTFKWMVNDEAGTLKYLGNRSDHEFVFPPTQEFEWKRARRDMYRYGAHPHISI
                     EDRVFVETIGGDLTVKVENNTDSGRGIYSEPVDNADQTLDDAEVHYAIVGNLILLKVL
                     PYQEKTWRHLVFNERTREAHRVDSIEQSCVLLPDGHGIIFPHGYVLQTGEVKRFDTGL
                     PPMRFERRVTAANGEDTLFVFTDLESGHYLLLAYNLITQSIATPITCQGYSLFPSGEL
                     VLFQADDEPRKHHVVQSWRTPFITADAAPHAQSQSFLGKIGNAEIVRAMAECHGILNL
                     LAKDDSFSGLYVELARSSGDITDSYFWIGKEEAHDLKSILSEIKSTAEAALGEFEKVS
                     RMRKNAAEQTAALQKKAAKSIAASTHGDPADILGFVHLLSALRELRGQIIALKDVRYV
                     DADEVARMDSGVAEAADKLSQKCVAFLLREEALDPYRRQISEQQARVPSLAKVLEATE
                     VEQALAQSSTELEMLTEIVSGLKIEDATETTRIIEHISALFAQLNQVRSVLRNRRNDL
                     AKAEGAAQFHAQLSLLGQSVLNYLEVATTPEKCDESLTRVMVQLEEMESRFSDFDDYA
                     AELTLKREEIQTSFEARRQSLVDALNRRCQSLGQSAERILASVRNRLSAFAKPEEVHA
                     WIAGDAMVAKLRDLIEELRKLGDSVRADDLQTKLKTLQQDSLKQIRDKADLYAEGGDV
                     IQLGRHKFSFNRQQLELTLLPREGRLVFHLTGTRYFEEVADPRLDELRHVWEQTSVSE
                     NAQVYRAEYLAWQIHKTHAPDAAVDVPAFMAQRYHEGYTKGVHDHDAALILEPLRAME
                     KALGPLRHAPAARGLALLFWHAWPEDEEKKALAARMRSKGRMRELLGISGADVQDSPA
                     ASIEAFLQQLASASPQSWCPGFSRSEAIAACLLEELQHLGPRHPALPFTRSAAAVELI
                     RAFRKELVVKMAAKEFDETLAALEAQPVTAFVVAREWLAALHPGTDGGVLTEAAALLV
                     LRDSPPAVTPAEPATRASLTGFTGTHSRIHEAALELDYHEFTARLARYEKGVVPDYER
                     FQQLKHETAVQRRAQLRLEQFKAGVLSSFVRNRLIDQVYLPIIGANLAKQIGAVGADT
                     RTDRMGLLLLISPPGYGKTTLMEYVAARLGITLVKINGPALGHKVTSLDPAEAPNASA
                     REEVERLNLALEMGDNVMIYLDDIQHCNPEFLQKFIPLCDGTRRIEGVFKGVAKTHDL
                     RGRKVAVVMAGNPYTEVGGKFQVPDMLANRADTYNLGDILGGHQEAFKDSYIENALTS
                     NPALARVAARSHADALACLKIALTGSREGIEFEANQSPEDINDCVAVMEKLLRVREVI
                     LRVNQEYIRSAAMEDAYRTEPPFKLQGSYRNMNKIAEKVQPLMTDAEVDALIADHYRG
                     ESQTLSQAAEANLLKWREINGLLDEPAAARWDEIKRTFGRNLLTGGAGESDPVARITG
                     QMSAFTVGLEKIERAVASPTLSDLTIERLQKIIEGLRAVPVNVEIKVQPVEKESAEEL
                     PVSIDSRVQQPSADFTDSAEKKGVRKKGRG"
     gene            17753..18199
                     /locus_tag="PLP58_08180"
     CDS             17753..18199
                     /locus_tag="PLP58_08180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ08529.1"
                     /translation="MTNILCSFEAAAHKEFSPVFRRIEFHRITLADALQEIQKLSQQD
                     DLAGKGLNIVLLPEGLNPIQTNARFSLVAEDVEYFDLFDAICHRLGLGCRMDPHALVF
                     ASANQIAQPHARSEASVKLARQIQLHLTLDGSDSITGADLKSGARQ"
     gene            18378..19847
                     /locus_tag="PLP58_08185"
     CDS             18378..19847
                     /locus_tag="PLP58_08185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009964229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HRJ08530.1"
                     /translation="MSSAPKLNRTQWLICIIASIGFAFDIYELLMLPLIVKPALASLG
                     GIGPDGAPLLLPGSPEFTQWARMLFFIPAIAGGIFGLLGGYLTDRLGRRRVLTFSILL
                     YAFSACAAGFSTTLWQLLFFRCLVFIGVCVEFVAAVAWLAELFKDNQQRERVLGYTQA
                     FSSVGGLLVAAANVLAAKWALDLPAIHGGHEAWRYTLISGVIPALPLILIRPFLPESP
                     EWARKKSEGTLKRASIRELFAPELVRTTVLTTLVFAASYGIAFGAIQQLPQILGAQAL
                     NTPNEAGHAAIIGTAKAAVGKAVQEAAAAGKPEIPAGKKRQIGGNASDEAVAKITFWQ
                     EIGGMMGRLALAILTVMIVSRRALFRVFQIPSLFFVPLLFWWISGQLANPDSLGLIKI
                     GIFVAGFLVVAQFSFWGNYIPLVFPMHLRGTGESFAANIGGRILGTAAAWITLTLAAS
                     DKPDPARIALVGACVAGGYALIGAILTQFLPEPEAEHQV"
     gene            19866..20111
                     /locus_tag="PLP58_08190"
     CDS             19866..20111
                     /locus_tag="PLP58_08190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020493876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin family protein"
                     /protein_id="HRJ08531.1"
                     /translation="MPVPVLYVKTGCPWCDEVLDFLDERRLPYRKVVVSGNAAAMREM
                     ADLSGQTKAPTMDWDGEVLADFGVDELIPFLKARNAL"
     gene            20522..21604
                     /locus_tag="PLP58_08195"
     CDS             20522..21604
                     /locus_tag="PLP58_08195"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009962734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0009263 - deoxyribonucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleotide-diphosphate reductase subunit
                     beta"
                     /protein_id="HRJ08532.1"
                     /translation="MEKIYKIGNREFPLDQSKAEAAYAAKKVINGRQSMTFNLLPLKY
                     QWAYDLYRTMKANHWEPEDIQMQKDVEQWRGHEISQNERWIIMMGIGYFSAAEGIVGD
                     NIQHVVRELVTAPELKLVLGRHAHEENIHADSLLYMISSLGINPHECEAMFEQIPTIV
                     RKNEFVTRHSNNLRRDLDLTKLENKQLLAKNIFVFGQCMEGTQFYGLFGMILSLYRQN
                     KFPGIGQMFRYTLRDESNHIEVFRNLFMDLVEENPDLWTAEFREELVQIMREAIALEK
                     EFIRDCLPVNAVGLSAGEFERYIDYIADRRLAGCGLQVLNPGVQNPLPWLAEMMDVRK
                     EQNFFEGKVTDYQKASALVHTSDDDL"
     gene            21720..>24245
                     /locus_tag="PLP58_08200"
     CDS             21720..>24245
                     /locus_tag="PLP58_08200"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018969880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005971 - ribonucleoside-diphosphate
                     reductase complex [Evidence IEA];
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_process: GO:0009265 - 2'-deoxyribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleoside-diphosphate reductase subunit
                     alpha"
                     /protein_id="HRJ08533.1"
                     /translation="MLQNLLHEDKALKKVAHTPKDQKPRFEWSAIAAGVTGSAPTAIK
                     VKVGGDERDFDMGEIADTIGSALTDLLLARQNDQDIYNDQNRRLVLTILTAVLEEIQQ
                     QAGAQAGAQAGANGGATFAARDIYQCIERALVRHSAHDIARSLAERRKRAEYDSLADN
                     TLPQPLIVNTKVIRRSGQLVPWNHNKIEIAVRKAFLSLELDSTPAVQIAEAVSGAVAA
                     ENKQFMHIEDVQNLVEEELMKQGYFKVARSYIQYRALRGKMREAEEQEAAGQNDIESQ
                     DQQSLIVVKTSDGGSFLWDGQDLKRRIDFAMLGLDLCLTRAEIEMELRRSLNSDITLD
                     HLKKTVILNAKTLMQKDADFAKFAARVLLSYIYEEVLGWDIVRDGIDQLREFHRRAFR
                     RNLARGIEIDRYNPRLLQFDLDKLADALDPAADLDFDFLGIQTLYDRYLIVDKKVKPS
                     RRLETPQLFWMRVAMGLCVQEDSPEEKIISLYKLYKGRRFCSSTPTLFNSGTHHSQLS
                     SCYLYKVDDSIESIMIRGIAENAFLSKWAGGLGGSWTSVRGTGGYIKGTNGESQGVIP
                     FLKLHNDQLIAVNQGGKRRGSGCAYLEVWHNDIEDFLQLRVNTGDERRRTHDLNTANW
                     IPDLFMKRVESRQPWTLFRANEVPDLHDLYGSAFEKRYEEYEALAKAGKIFGKETPAI
                     DLWKLMLKALFETGHPWITFKDPCNLRSPQDHTGVIHSSNLCTEITLNTSADETAVCN
                     LGSVLIDNHLDDAGGMDHTKLRETIRTAVRMLDNVIDINFYPTEAARTANSRHRPIGL
                     GVMGLQYALYRKGIPFASREAVEFNDEFMEAVAYYAYEASSDLA"
BASE COUNT         4603 a         8394 c         7081 g         4167 t
ORIGIN      
        1 cgggcatcgg cgcgggcgcg ctggcgctgg cgtggctgct gggcggcgtt ttttcccgcc
       61 agctcagccg gcccatccac acgctggtgg cggccacgca ggcggtgcga gggggggatt
      121 tcgagacacg actgccggag gccggcacgg atgagtttgc ggagctgaac ctctccttca
      181 acgagatgac cgagggcctg gcgctcaagg agcgctacca ctccgtgctc agcatggtca
      241 ccgacgcgcg cgtggccgag cagatgatgg cgggcaagat caagctcggc ggcgaggcgc
      301 gggaggcgag catcatgttt tgcgacatcc gcggctacac cgccatgagc gcggggcggg
      361 acccggcgga ggtcatcgcc attttaaacc gccacatggg cgcgctgaca gaggtggtgt
      421 accgctggca cggcgtcatc acccagttcg ccggggatgc catcatggcc gtcttcggcg
      481 cgccaacgca tcatggcgag gacgcggtga acgccgcgcg ctgcgcgctg gagatgctgg
      541 cggcgcgcgc gcggctgaac gagggctcgg ccctgcccct gggcattggc atcggcatcg
      601 ccactggaaa tgtcgtggcc ggctgcatcg gcgcggagaa acgcgcggac tacaccgtgg
      661 tgggcgagcg cgtgaacctg gccgcccgcc tgtgcggcgc ggccgccgcc ggccaggtgg
      721 tgattgatga gaccacccgc gcgcgcctgc ccggagatgt cgtcaccgag gctctgccgg
      781 agccgttaaa attgaaaggc ttcaccgacc ccgttccagc gtcgcgggtg ctgcgcctta
      841 attcccaatg atcatgcgtt tcctcgccat taccgccgcc ttgtttgcca tgcaggccgg
      901 tctctgccgc gcgcaggcct tcctggagac tctggatgag gcgctctccc tgcacagcac
      961 ggacgccttc tggcgggcgg atgtgtcggg cctgctcgat ctggaaacct atgtgccgga
     1021 gactcccgcg ctgggcctgc tcaacacgga cgacgaggtc tttttcaatc cgcgtctctc
     1081 gctattcctg gatctgcgcg ctggcgagac gctgcgcgcc catgcgcaga tgcgggtgga
     1141 tcgcggcttc gatcccggct tcgcgccgga cgggcagacg cggctggacg agtacttcat
     1201 cgagtggcgt ccgctgggcg atgacgcgct ggccttccgc gcgggcaagt cggccaccgt
     1261 cttcggcgcc tggactcggc ggcatttctc ctgggacaac cccttcgtca ccgcgcccgc
     1321 cgcctactcc gacatgctgc ctgtggtgga ccatcgcgcc gcgcccaggg gcgccctgct
     1381 cggccgccgc ggcgagcggg acaatccact gacctggctg cctgtggtgt ggggaccgtc
     1441 ttatgccacg gggctgtccc ttttcggcca ggtgtcggat ttcgactacg cgctggagat
     1501 aaaaaacgcc gcgctctcct cccgcccgca cacctgggac gccgcgcaaa acggctggac
     1561 cagcaccacc tggacgggcc gcctcggctg gaaacccgcg cccgagtggg ccttcggcgg
     1621 ttctttcagc cacgggccgt atttgctgga ggacgcccgg cgcctgccgc gcggccgggg
     1681 cttcgaggac tacgcgcaga ccaccttcgc cctcgacgcc gcgtgggcgc acggcccgtg
     1741 gcaggtgtgg ggagagctga tgaccagccg cttcgccatc ccgaacgccg gcggcgcgcg
     1801 cgtgctcacc ggcttcatcg aggcgaagcg caagctcggc tccggctcgt ggctcgcggc
     1861 gcgctggaat caaagctggt tcggcgacgt gcccggcacc tccaccgcct gggacaacga
     1921 cggctggcgc gccgacctcg cgctcggcca ccgcctcagc cgccatctcc aggccaagct
     1981 gcaatacagc ctcgccggcc gctccggccc tcatgatgaa ggggagcatc tgctggccgc
     2041 gcaggtcacg gtgcgttttt gaccgcctcg aaatgacggc aggcgcaaac aattccaaag
     2101 gctgaccgtt cccatgctct ccgccatgaa gcctctcctc gtctcttttc tcctgcccgc
     2161 gctcgccctc gcgcagcccg cgcccaaggc caaaaaggcc gcgtcctcca tgccggaggg
     2221 ctttcacgaa ctcgcgctgg gagatgcggc gccggatttc gcactgcctg gcattgatga
     2281 gaaaacgcac accttgaagg actacgccgg cgcgcgcctg ctcatggtgg ccttcatcag
     2341 caaccactgc cccagctcgc aggccatcga ggcgcggctg aaaaaattca ccgccgctta
     2401 tgagggcaaa ggcgtgcagg tcgtggccat caatcccaac gaccccgccg ccctgcgccc
     2461 ggacgagctc ggctactcca agtacaacga cagcttcccc gagatgaagc gccacgccgc
     2521 ggagcagggc ttcaatttcc cctacctcta cgatggcgag acgcagaaga ccgcgctggc
     2581 ctacggctgc ctggccaccc cgcatgtctt ccttttcgac caggagcgca agctgcgcta
     2641 caagggccgc ttcgacgaca gccgcttcgc cgacgaggcc agtgtgacct cgcatgacgc
     2701 gcgcaacgcc gtggacgacc tgctcgccgg gcggcccgta gcgctcgccg agacgcgccc
     2761 gcacggctgc tcgacgaagt ggatcaccaa gcgagaggcc gtggccgccg atcagacgaa
     2821 atgggagacg actcccgtgg atgtggatct cattgacgcc aaaggcgtgg ccgcgctgcg
     2881 caaaaacgac acgaagaaag tgcgcctctt caatgtcttc gccacctggt gcggaccgtg
     2941 cgtggaggag ttccctgaac tggtgaacac gcagaggaag ttcgccctgc gcgacttcga
     3001 gctcatctgc atcagcatca acgagccgga tgaaatgcag gaggtgaagg agttcctgga
     3061 ggagcagaac tgcatcgtgc cggacaaatt aaaaccctcc ctcaaagccg agggccgcaa
     3121 aggcaacgcc tacgtcttcc aaggcgcgaa ccaggacgaa ctcatcgccg cgctcgaccc
     3181 cgaatggccg ggccccatcc cgcacacgct cgtcgtcgcc cccggcggag aagtcatctt
     3241 ccgccacaat ggcgaggtgg atggcgacga gttgcgcgcg aagattctcg agcacatggg
     3301 gcgctacttc acgccggaag attgaactca cgactcatga aaaaactcct tctttccctt
     3361 ctgatgccct gcgccctgca cgcgcaggag gccagatcgc ccaatgagct gttccgcgag
     3421 ggcgtggccc tctttttcga ggcgaagccc agggaatccg tggccgcttt tgacaagctg
     3481 ctcgaactgg agccgggcgc gaaaccgaag ctctggcagc gcgggctggc gctatattac
     3541 gcggaagatt accagggcgg gcgcgagcag ttcgaggtgc atcagaccgt gaacaccgcc
     3601 gatgtcgaaa acgccgcgtg gcatttcatc tgcgtggcga aaaaggaggg cgtggaggag
     3661 gcgcgcaagc acctcatccc catctccggg gacagccgcg tgcccatgaa gcaggtgcat
     3721 gacctctttg cctccaaagc cacggtggac gacgtgctcg ccgccgccga gcagggggag
     3781 ggcgaggact tgcgcaacca ccgctgctac gcccacctct atctcggcct ctacttcgag
     3841 gcgctggggc aggaggcgaa ggcgaaggag cacatcctca aagccgccgt ggatttctcc
     3901 atggaccatt acatgggccg ctgctcgcag gtgcatgccc gggtgcgcgg ctgggacgca
     3961 aaaaaagagt gacggcacgc gccgtcactc ttcacggatt ggcaatgcgc gggtcactcc
     4021 ttcgcggcct tcgtccagcc gccgaagttg gcgccggcgg cgatccattt cttgatcagc
     4081 tcctggtcgg cggcgctgac gcggtcgccc ttgccttctg gcggcatggc atactcgtcg
     4141 gattccggca gggccatggt cttgagcagc cagctgtcgt caggcttgcc ggccaccaca
     4201 ttttccttcg ggtattcctt gcctccggcc atgatgccct cggcggtgtc gagcaccagt
     4261 ccgcctttcg ggcgcttcac ccggccgtcc tcctcatgct cggtctcgtg gcatttgaag
     4321 cagctggtct tcaatacggg gaggatttgt ttttcaaagc tgatgtcctc cgcggcggcg
     4381 aggctggtgg agacaatgag ggcggcaagg gcggtttgga ttttcatgcg cgcggaaaat
     4441 gggcggcccc ccgcgcgcgg tcaagcggaa gcacgagact cttgcagacc gcggtcacag
     4501 cttcgtcacg atgaagttta tggcacggat gcgtctctcc tccctccctc acgccgccct
     4561 ctgcaccgcc caaaacagac cgcacagcgc ggtgcatgcc agcgcgggca gggccacgcg
     4621 cacgcggtac cagggccgcc gccaccaggg gctgaagacg agcatgagcg cgcccaccac
     4681 gacgagctgg cccaactcca caccgagatt gaacccaaag agcgcggaca cagggctgcg
     4741 gcggatgccc tcctcctgga cgagattgtg ggcaaaggcc agcccgtgaa tcatgccgaa
     4801 agcgatgacg agcagccagc gccagcgtgc caggctgcat gaaaacatgc cttccaccgc
     4861 cacggcggcg atgctcagcc ccacggccac ctccacccag cgcggctcaa agctgaagcc
     4921 ggtgagcacc accagcccca gagtcaaaga atgcgccagg gtgaagatcg tgacctgcgc
     4981 gagcagcgcg gggaaggcgc gggagagaaa gaacagcccg agcaggaagg cgatgtggtc
     5041 cagcccctcg gggatgatgt ggcggaagcc ggacgccagc ccggcgccca gcacttccaa
     5101 gtccagcatc ccgcgcgcca gcgcgagcaa ctcctgaacg ccgagcacca ggagcatcgt
     5161 gagagcggag actgccgtga ggcttttcat ttggaagcgg cgcgcactct cccccgaccc
     5221 gatgatgccg cgagtggaat ttttcttttc agatccaggc ggcggctgca agctcgcgcc
     5281 catgtcccat cgcatccatg acatgccgga agacgaccgc ccgcgcgagc gcctgctgcg
     5341 cctcggcccc ggcgcgctct ccgacgccga gcttctcgcc attttcatca acacgggcgt
     5401 gaaaggagag aacgccctcc aggtggcgaa ccgcctgctc cgcgaggtgg gcaccctgca
     5461 caacctctcg cgcacctccg ccagccagat cgccgcctcg cacgcgctcg gcccggcgaa
     5521 ggccgcgcac ctggccgccg ccttcgagct ggggaggcgc gcggagcgct tttgggtgaa
     5581 ggacacgccg ctggacgagc cggaaaaggt ctatcgctac ctcgccgccg agatgcagag
     5641 cctgccccac gagacgctgc gcgtgctgct gctgaacgcg cgcttccatc tcatgcgcga
     5701 cgaggtcatc ttcaccggca cggtcaatga gaccatggcc cacccgcgcg acatcctgcg
     5761 ccccgccgtc atccacaagg cctacggctt caccgtcgtc cacaaccacc cctcgggcga
     5821 ccccgctccg agcacggcag accgcgcctt tacccggaag atcaaggacg cggccgaggt
     5881 gctgagcatc aagttttgcg accacgtcat catcggcatg cccgaccaga gcggccgaaa
     5941 gccctacttc agcttccgcg agatgggcgc cgtatagccg cgcttgcttt cgcattccct
     6001 cgaacgcgcc tgcgtggggc cgcccgcttg cttttccaca ggcgcggcgg atggtcggcc
     6061 catgctgctg atcatagaca attacgactc cttcacctac aacctcgtcc agtacttcgg
     6121 cgagatgggc gcgcgcatgc agatccaccg caacgaccag atcacgctcg aggaaatccg
     6181 ccgcatgaat ccggaccaca tctgcgtctc gcccggccca tgcacgccga aagaggcggg
     6241 catcagttgc gatgtgatcc gcgcttttgg cgggagcacg cccatcctcg gcgtgtgcct
     6301 ggggcatcaa agcatcggcc atgtcttcgg cggggatgtg gtgcgcgcgg ggcggctcat
     6361 gcatggcaag acctccatga ttcaccacac gggcaaatcc gtcttcaaag acctgccgga
     6421 cccctttgaa gccacgcgtt accactccct gctggtgaag cgcgacacac tgcccgagtg
     6481 cctggagatc accgccgtcg ccggcgatga cgagagcgag atcatgggcc tgcggcacaa
     6541 ggagctgccc atccacggcg tgcagttcca ccccgagtcc atcctgacca ccgaggggaa
     6601 aaagctgctg cgaaacttcc tggagatgtg agcctcagcc gcccttgctg ggcaggtcca
     6661 cgagcatctg cgcgttgctt gggtagcgct ccatgagggt gagcacgcgc tggatggcgt
     6721 ctatcggctt gtggccggcg aggccgcggc gcagcatgtg gatcttgtcg agctggaagg
     6781 actgaagcag cagctcctcg cggcgcgttc cggatttgaa gatgttcacc gcgggataat
     6841 aaaactgctc ggcgatcttg cggtcgagca cgagctccat gttccccgtg cctttgaact
     6901 cctggaagat caggtcgtcc atgcggctgc cggtctcaac gagggcggtg gccaggatgg
     6961 tgaggctgcc ggcctcgcgc gtgttgcgcg cggcggcgaa aaggcggcgc gggatttcca
     7021 tcgcgcccac gcccatgccg ccgctcatgg tggcgccgcc ggacttggcg ttgttaaagg
     7081 cgcgcgccag gcgggtgatg gaatccatga gcatgaagac atgctcgccg cactcgacga
     7141 gacgcttcgc gcgctcgatg gcgagcgtgg cgatgcgcgt gtggctcttc acatcctggt
     7201 cgttggagct ggcgtaaatc tcggccttgg gcaggatgcg tttgaactcg gtgacctcct
     7261 ccggacgctc gtccacgagc aggatcatca agtggatggc ggggtggttc gccaccacgg
     7321 cttccgcgat gtgctggagc atcgtcgtct tgcccgcacg cggcggggcg acgatgaggc
     7381 cgcgctggcc gcgtcccacc ggggtgaaca tgtctatcac ccgcgtggtg agacggtctt
     7441 ttgtggtttc aagctggagc tttttgcagg gattggtggc cttcagctcc tcgaacaagg
     7501 gcagatcgcg catggcttcc ggcgcgcggt cgttcacctc gccgacgccg gtgatctgcg
     7561 gtccgcgcgg gcctttttgc gcgatgcctt tcaccgccat gccctcgcgc aggccatact
     7621 ggcggatgaa gtcgccggag atgaaggcgt cctgcggatg ctgggcatag ccaagctcgc
     7681 ggcggcggag aaagccgtag cctttgggcg tgagttccag gacgccctcc gcgggctcgg
     7741 gcgggccgag cacaacttct ggtgtgcgtg atctttcgtc ctcgccgttg cctgcgtccg
     7801 ggtccgctcc ggcctccccg gcggcctgct gcgcggcgag cctgcgcgcc agcttctcct
     7861 ggcggaagcg ttcgcggcgt tctttccagc gctcgaattt cgacttcttc cgctcgggct
     7921 gcgcgggagg cgccgcctcc ccgtctggcg cctcgttttc taccgcggga gctgtctccg
     7981 gcacggatgt ctcaatggat actggcgcgg atgtctcgac catcgctgga gcggcggcag
     8041 gtgccacgga tggttgctcg acagcaggcg gcggctccgg ttcggatggc gctatctcaa
     8101 tggcagggga ctcgggattc ttggcggcag gcgccgcgtt cagcgccgcc ttctgcattt
     8161 ttttgaggat gctggcggct tttttcacca cggttaccga cttgcgcggg agcttgggct
     8221 cggcgggcgt ctcagtctct gcttgagcgg caactggatt ttcctcaggc gcggtaacct
     8281 cgacttcaac ggcggcctgg gttttcgcgg cttttttagc cggggctttt ttcaccgccg
     8341 cctttttggc ggcggttttc ttggcggcgg cctttttggc gggtgtcttt ttggcgggtg
     8401 tcttttttgc ggcggccttt ttcacaggcg gctcggatga cggcaggtcg agcaggagtt
     8461 cttcgctcat ggttttggaa agggagggtt caagagagcg cgtcggcgat ctcgtcggag
     8521 atttcgaaat tggagtggac gttctgcgtg tcgtcgtagt cgtcgagctg gtcgtggagt
     8581 ttgatcacgg ccttggcctg gtcgagatcg gtgagaatga cgggggtcgc cgcctggtaa
     8641 atgagcttct gcgatgtgct ttcatgaccc ttgtcacgca gcgcgccggc gacttggaaa
     8701 agctggtcgg tggctgtgta gagaagccat tcctcatccg catcctcgac atcctcggcc
     8761 cctgattcga gcgccagttc catcgcgccg tcctcgtcga ggccgccttt gggcagccgt
     8821 atctcgcccc tgcgggtgaa ctggtaggcc acgctgccgg catcggcgaa ggtgccgccg
     8881 ttctttgaaa gcaggacgcg caggtcgttg gcggcgcggt tgcggttgtc tgtcacgacc
     8941 tcgatcatga aggccactcc gccggggccg tagccctcgt atgtgacctc ctcaatggcg
     9001 tcgcccccca gctcgcccgc gccttttttg atcgcgcgct cgatgttgtc attcggcaca
     9061 ttggcggcgc gcgcggccag gatggcgctg cgcagccggg cgttgagatc cggattcgag
     9121 ccgccgtgct tggcggcgac agtcagctcc ttggagagct tgctgaagac cttgccgcgc
     9181 ttcacgtcca cgacggcttt gatatgcttc accttggacc atttgttatg tccagccatg
     9241 ttgctttgcg gaaatctgag aaggttgggg taacgcggaa gaatgccggc ttttgcgggc
     9301 ctggggtcct cgtcgcggag gggagaattg gatggggggg aatgagagcg gcctcgccgc
     9361 ggtttccact cgaaacaagc cggagcttgc cgcaggaacg ggtttcgagg acagcgcgcc
     9421 tgtgaaacca caactccgaa tcgccggagc ggacaccaat gttcacctgg ctgagagagc
     9481 caggaacaat gggagtgtgc gggagtcccc gcgtccttgc aaggtgtttt ttccgggttg
     9541 gaccgtccat tgcggcttgc cagcgttctc ctccatgcct tcaaacctgc ctgcgggctt
     9601 gatggccgct ggcaagcgtg tggaaaattt tgcggatttt gtagtgtgct tgtttatccg
     9661 ggctgcttaa ctcgccgcgt ttcatgccat gatcgagctc taccaggaaa tcatcgcgcc
     9721 gcataacttg ccggtcacct tgctgcttgg cgcggtgttt ttgtactggc tgctggtgct
     9781 cgcgggcatc ctggactttg acctcgacat cctggagatc ggtgatgcga ccgccgacct
     9841 gggtgacgcc gccgacgcgc accacggcca tctcggcggc ttgtggctca gctttggcaa
     9901 ggcgctcggc ttcgccaagg tgcccatcct catctggggg agcttttttt cactcttcct
     9961 ctgggcgggc aatgtggcgc tgaactatta ttacaacggc agtccgggtg agcgcagcat
    10021 gtccaccgcc gcgctgcttt ttgtgcctgg agtgctcggc agtctcgtgg tcaccaagat
    10081 cgtcaccctg cccatcggca gacttttcac cgccatggcc gagtcggcca cggaaaatgt
    10141 ggatgtcatc ggccggaccg gagtggtcgt cagcgccgag gccaccgacc agttcggcca
    10201 gctccagatc ggcaatgaag gcgcgccggt gctgctgaac gtccgtctca ctcccggcgg
    10261 cgcgcctctg acgaagggcg cctccgccaa aatcatcacc gcctcccccg acggcaaatt
    10321 ttacctcatc gaacccgcct gacccgctca tccatcgaac cctcaaaccg ccaacactcc
    10381 cgccatgcac acacccatcg ccatcatcga actcctcggc ctgccggtca tcggcggcat
    10441 tgtcggcctc atcgccggcg tcatcatcct catctcgcgt ttctaccgga aggtgcatca
    10501 aggccaggcc atcgtgcgca acggcatcgg cggaaccaag gtctccttca acggcatgct
    10561 cgtcatcccc atcgcgcatc atgccgagta catggacatc tccgtcaagc gcgtggagat
    10621 cgagcgccgc gggccggaag ggctgatctg caaggacaac atgcgcgctg acatcaaggt
    10681 ggcctttttc gtccgtgtga acaagaccaa cgaggacgtg ctgcgtgtgg cccagagcat
    10741 cggatgccag cgcgcgtcga gtgtggacac catgcgcacc ttgttcgacg ccaaattctc
    10801 cgaggctctc aaaaccgtgg gcaagcgttt cgagttcgtc cagctctata atgagcgcga
    10861 ccacttcaag aacgagatcc tcaaggtcat cggcaccgac ctcaacggct tcaccctcga
    10921 cgactgcgcc attgacttcc tggagcagac ccagctcgaa aaccttgatc cgcagaacat
    10981 cctcgactcc gagggcatca aaaaaatcac cgagctcacc gccacccagg ccaagctcgc
    11041 caaccacatc cagcgcgaca aggaaaaggt catcaagcag caggacgtgc aggcccgcga
    11101 ggccatcctc gaacttgagc gccagctcgc cgagaccgag gccaagcaga agcgtgacgt
    11161 ggaaagcgtt aaagcccgtg aagaagcgga gacactcaaa gtgcaggctg aggagcgcct
    11221 gaaggccgag cgcgccaaga tcgccgccga tgaggagatc gccatcgcca acgaaaacat
    11281 ggagcgccag gtgctcgtgg cccagcgcag caaggagcgc acggacgccg tcgaagtcga
    11341 gcgcgtcacc cgcgaccgcg acctggagat catcgagcgc gagcgcgtga ccacactgaa
    11401 ggacatcgaa aaagagaagg ctgtcgaagt cgagaagaga aacatccagg aggtcatcaa
    11461 agagcgtgtg gcgctggaga agaccgtcgt cgtcgagcag cagaagatca aagacaccga
    11521 ggccttcgcc accgccgacc gcgagaagca ggtggctctg accctggccg aaaaagccgc
    11581 ccaggagcag ctcatccaaa agatcaagga agcagaggcc gcgaaggaag ccgcccgcct
    11641 tgccgccgag caggacgcct ttaccacggt caaggaagcc gatgccgcca aggaggccgc
    11701 ccagctccgt gcccaggaaa tgctcaccct ggccgaagcc aagcaagaag ctgccgcacg
    11761 cgaagccgcc gccgaaaaag cgctcgccga agccgtcgcc gccaagaccg ccgcgcccgg
    11821 actcggcgag gcccaggttc ttcttgccaa ggccgaggcc gcgcagaagc agggccatgt
    11881 ggatgccgag gtgcttcagc tcaagctcgc cgccgaagcc gacggcatca ccaagaaagc
    11941 ccaggccatg aagctgctcg aagacgccgg acgcgagcac gaggagttca agctcacgct
    12001 cgacaaggag aagtccgtcg agctcgccca gatccacatc cagaaagaca tcgccgaagc
    12061 ccaggccggt gtgcttggcg aggcgatgaa atccgccaag atcgagatcg tcggcggcga
    12121 gagccagttc ttcgacaaga tcaccagcgc catcggcaac gcccgcgccg tggaccgcct
    12181 cgtcgaaggc agccgcacgc tcagcgatgt gaaggagacc ttcttcaatg gcgacccgga
    12241 ctacttcaaa gcgcagttga agaccctcgt ggaccagttt ggcctcaccg ccgaggatat
    12301 taaaaacctc accctttccg gcctcttcgc caagctcatc ggcctcaacc ccgacgccga
    12361 aacgctgaac aagctcaccg gcatgatcgg cgccgccacc cgcttcggcc tcgccgagca
    12421 aagcgtcggc gcgttgctca cgggcaaggc gaagaagtga cgcattcggc tttccacttc
    12481 accgactgat gatctgccgt cacttcaccg gcgctgtcca tggctgaacc aactccatcc
    12541 ccccaggccg cctctcccca gctcgaagcc ggtgcttacg aggtgatccg gcagcggctg
    12601 gacaagcatg gggcggagct gcaaaggcgg ctggatttgc tgaacaacga ccgcaaagcc
    12661 gagttcggcg gcattgacac cgcgctgctg gccaccacgc gcctgaccac ggacaacaac
    12721 tgcgtgccgc gcgacatgac ggccatcggc ccgcggcgct ttttgttcgg ctacaatgtc
    12781 cacctcggcc tgcgctcgca gatgcatgtg gaggatgtct tcgcgggcta cgactacaat
    12841 cccgaggacc acagcttcca tcccatcaag gacgggctgc tctccgatcc gcagttcgtc
    12901 gaggacttcg cctacctcta cggctactac aagaacgcct cctttctgaa gttccaccgc
    12961 atcggcccgc acctctacat gggcatgtcc gtcgggcgcg gcgcggcgga ggtgaagacc
    13021 ttcaaatgga tggtcaatga cgaggcgggc acgctcaaat atctcggcaa ccgctccgac
    13081 cacgagtttg tctttccgcc gacgcaggag ttcgagtgga agcgcgcgcg gcgggacatg
    13141 taccgctacg gcgcgcatcc gcacatcagc attgaggacc gcgtttttgt cgagaccatc
    13201 ggcggcgacc tcacggtgaa ggtggaaaac aacaccgact ccggcagagg catctacagc
    13261 gagccggtgg acaatgccga ccagacgctc gacgacgccg aggtccacta cgccatcgtc
    13321 ggcaatctca tcctgctcaa agtgctgccc taccaggaga aaacgtggcg gcatctcgtc
    13381 ttcaacgagc gcacccgcga ggcgcaccgc gtggacagca tcgagcaatc ctgcgttctg
    13441 ctgccggacg gccacggcat catcttcccg cacggctatg tgctgcaaac gggcgaggtg
    13501 aagcgcttcg acaccggcct gccgcccatg cgcttcgagc gccgcgtcac cgccgccaat
    13561 ggcgaggaca cgctctttgt cttcaccgat ctcgaaagcg gccactacct gctgctggcc
    13621 tacaacctca tcacgcagag catcgccacg cccatcacct gccagggcta cagcctcttc
    13681 ccctcgggcg agctggtgct ctttcaggct gatgacgagc cgcgcaagca ccatgtcgtc
    13741 cagtcctggc gcacgccttt catcaccgcc gatgccgcgc cgcatgcgca gagccagagc
    13801 ttcctcggca agatcggcaa tgccgagatc gtccgcgcca tggccgagtg ccacggcatt
    13861 ctcaacctgc tggccaagga cgactccttc tccggcctct atgtcgagct ggcacgctcc
    13921 tcgggcgaca tcacagacag ctacttttgg ataggcaagg aggaggcaca cgatctcaag
    13981 agcatcctgt ccgagatcaa gagcaccgcc gaggccgcgc tgggcgagtt tgaaaaagtc
    14041 agccggatgc gcaaaaacgc cgccgagcag accgccgcgc tccagaagaa agccgcgaaa
    14101 agcatcgccg cctccacgca tggcgacccc gcggatattc tcggctttgt ccacctgctc
    14161 tccgccctgc gcgagctgcg cgggcagatc atcgccttga aggacgtgcg ctatgtggat
    14221 gccgacgagg tggcgcgcat ggacagcggc gtggccgagg cggcggacaa gctctcacag
    14281 aagtgcgtgg ccttcctttt gcgcgaagag gcgctcgatc cctaccgccg ccagatcagc
    14341 gaacagcagg cgcgcgtgcc gagcctcgcg aaagtgctgg aggccaccga ggtggagcag
    14401 gccctcgcgc agtccagcac cgagctggag atgctcaccg agatcgtcag cggcctgaag
    14461 atcgaggacg ccaccgagac cacgcgcatc attgagcaca tctccgcgct ctttgcccag
    14521 ctcaaccagg tgcgcagcgt cctgcgcaac cgccgcaatg atctggccaa ggccgagggc
    14581 gcggcacagt tccacgctca gctcagcctg ctcggccaga gcgtgctgaa ctacctcgaa
    14641 gtcgccacca cgccggagaa gtgtgacgag tccctcacgc gcgtcatggt gcagctcgag
    14701 gaaatggaga gccgcttctc cgacttcgac gactacgccg ccgagctcac cctcaagcgc
    14761 gaggaaattc agacctcctt cgaagcgcgc cgccagtcgc tcgtggacgc gctgaaccgc
    14821 cgctgccagt ccctcggcca gtcggcggag cgcatcctcg ccagcgtgcg caaccgcctc
    14881 tccgcctttg ccaagccgga ggaagtccac gcctggatcg cgggcgatgc catggtggcc
    14941 aagctgcgcg acctcatcga ggagctgcgc aaactcggcg acagcgtccg cgccgatgac
    15001 cttcagacca agctgaagac cctccagcag gactcgctca agcagatccg cgacaaagcc
    15061 gacctctacg ccgagggcgg cgatgtcatc cagctcgggc ggcacaagtt cagcttcaac
    15121 cgccagcagc tcgagctgac cttgctgccg cgcgaggggc ggctcgtttt tcatctcacc
    15181 ggcacgcgct actttgagga ggtcgccgat ccgcgcctcg acgagctgcg ccacgtctgg
    15241 gagcagacct ccgtctcgga aaacgcgcaa gtctatcgcg ctgaatacct cgcctggcag
    15301 atccacaaaa cccacgcgcc cgatgcagcc gtggatgtgc ccgccttcat ggcgcagcgt
    15361 tatcacgagg gctacacgaa gggcgtacat gaccacgatg ccgcgctcat cctggagccg
    15421 ctgcgcgcca tggaaaaagc gctcggccct ttgcgccacg cacccgccgc gcgcggactc
    15481 gcgctgcttt tctggcacgc atggccggag gacgaggaaa aaaaagccct cgccgcgcgc
    15541 atgaggtcga agggccgaat gcgcgagctt ctcggcatca gcggcgcgga tgtgcaagac
    15601 tctcccgccg ccagcatcga ggcctttttg cagcagctcg ccagcgccag cccgcaaagc
    15661 tggtgtccag gctttagccg ctccgaagct atcgccgcct gccttctcga agaactccag
    15721 cacctcggcc cccggcatcc cgcgctgcct ttcacgcgct ccgccgctgc cgtcgagctg
    15781 atccgcgcct tccgcaaaga actcgtcgtc aagatggccg cgaaggagtt tgatgaaacc
    15841 ctcgccgcac tcgaagcgca gcccgtcacc gccttcgtcg tcgcgcgcga atggctcgcc
    15901 gccctgcatc ccggcaccga cggaggcgtg ctcaccgagg ccgccgcgct gctcgtgctg
    15961 cgtgattcgc cgcccgccgt cacgcctgcc gagcctgcca cacgcgcttc gttgaccgga
    16021 ttcaccggca ctcactcgcg catccatgaa gccgcgctgg agctggacta ccacgagttc
    16081 accgcgcgcc tcgcgcgtta cgaaaagggc gtcgtgccgg actacgagcg cttccagcag
    16141 ctcaagcacg agaccgccgt gcagcgccgc gcccagctcc ggctggagca gttcaaggcc
    16201 ggcgtgctca gctccttcgt gcgcaaccgg ctcattgacc aggtctatct gcccatcatc
    16261 ggcgccaacc tcgccaagca gatcggcgcc gtcggggccg acacgcgcac cgaccgcatg
    16321 ggcctgctgc tgctcatttc accgcccggc tatggcaaga ccacgctcat ggagtatgtg
    16381 gccgcgcgcc tcggcatcac gctggtgaag atcaacggcc ccgccctcgg ccacaaagtc
    16441 acctccctcg accccgccga agcgcccaat gccagcgcac gcgaggaagt cgagcgcctg
    16501 aacctcgccc tcgagatggg cgacaacgtg atgatctacc tggacgacat ccagcactgc
    16561 aatcccgagt ttttgcaaaa gttcatcccg ctttgcgatg gcacccgccg catcgaaggc
    16621 gtcttcaagg gcgtggcaaa aacccacgac ttgcgcggac gcaaagtggc cgtcgtcatg
    16681 gctggcaacc cctacaccga agtcggcggc aaattccagg tgcccgacat gctcgccaac
    16741 cgcgccgaca cctacaacct cggcgacatc ctcggcggtc accaggaggc cttcaaagac
    16801 agctacatcg agaacgccct caccagcaat cccgcgctcg cccgcgtggc cgctcgcagc
    16861 catgccgacg cgctggcctg tctgaagatc gccctcaccg gcagccgcga gggcattgaa
    16921 ttcgaggcca accaaagccc cgaggacatc aacgactgcg tcgccgtcat ggaaaagctg
    16981 ctgcgagtgc gcgaggtcat cctgcgtgtg aaccaggagt acatccgcag cgccgccatg
    17041 gaggacgcct accgcaccga gccgcccttc aagctccagg gcagctaccg gaacatgaac
    17101 aagatcgccg aaaaagtgca gccgctcatg accgatgccg aggtggacgc cctcatcgcc
    17161 gaccactacc ggggtgagag ccagaccctc agccaggccg ccgaggcgaa cctgctcaaa
    17221 tggcgcgaga tcaacggcct gctcgacgag cccgccgccg cgcgctggga cgagatcaag
    17281 cgcacctttg gccgcaacct gctcaccggc ggcgcgggcg agagcgaccc cgtcgcccgc
    17341 atcaccggcc agatgagcgc cttcaccgtc ggcctggaaa aaatcgagcg cgccgtcgcc
    17401 agccccaccc tttccgacct aaccatcgaa cgcctgcaaa agatcatcga aggcctgcgc
    17461 gccgtgcccg tgaacgtcga gatcaaagtc cagcccgtcg aaaaagagtc cgccgaggag
    17521 ttgcccgtct ccatcgactc gcgggtccag cagccatccg cagatttcac agattccgca
    17581 gagaagaagg gagtgcggaa gaaagggcgg gggtgaaagt ccgctctata gctgaacgga
    17641 gtcgtcaaaa ttacgcgcga acccatttac tcaagaagtg atgacaactt ggcctcatgc
    17701 tgaaccggtt ttgaagtcat ccgccgcata ctcaatcctc ctggcagtct tgatgacgaa
    17761 tattctgtgc tctttcgaag ctgcggctca caaggagttc agtccagttt ttcgccgcat
    17821 agaatttcac cggatcacac ttgctgacgc gcttcaggaa attcaaaaat taagtcaaca
    17881 agacgacctt gccggaaaag gactcaatat tgtcctgcta cccgaaggtc tgaacccaat
    17941 tcagaccaac gcacggttca gtcttgtcgc ggaggatgtg gagtatttcg atctctttga
    18001 tgccatctgc cacagattgg gtttgggctg caggatggat ccgcacgcgc tggtatttgc
    18061 atctgcaaac cagatcgccc agcctcatgc tcgcagcgaa gcatctgtaa aactggctcg
    18121 ccagattcaa cttcatctga ctctggacgg cagcgactcc atcacaggag ccgatctcaa
    18181 gtctggggca cgccaatagt ggatcccgcg ccggaaaggg aattgccaga aactctgcgt
    18241 gtgacaggca tttttcgttt caggcgcggg gacgaccgtt ttgcttcgct ccccgcattt
    18301 cccccccgtg gcgctgatgg attcccgccc aagcgcgcgt tgacttgcct cccaatccca
    18361 gacagcatcc gcgaatcatg tcctccgctc ccaaactgaa ccgcacccag tggctcatct
    18421 gcatcatcgc ctccattggc ttcgcctttg acatttacga gctgctcatg ctgccgctca
    18481 tcgtcaagcc cgctctcgcc tcccttggcg gcatcgggcc ggatggcgcg cccctgctgc
    18541 ttccgggctc gccggagttc acgcaatggg cgcggatgct ctttttcatc ccggccatcg
    18601 ccggcggcat cttcggcctg ctcggcggct acctcaccga ccgcctcggc cgccgccgcg
    18661 tgctcacctt cagcattttg ctctacgcct tctccgcctg cgcggcgggc ttttccacca
    18721 cgctctggca gcttcttttc ttccgctgcc tcgtcttcat cggggtctgc gtcgagtttg
    18781 tcgccgccgt ggcctggctg gcggagctgt tcaaggacaa ccaacagcgc gagcgcgtgc
    18841 tcggctacac ccaggccttc tcctccgtgg gcggcctgct cgtggccgcc gccaatgtgc
    18901 tcgcagccaa gtgggcgctt gatctccccg ccatccacgg cggtcatgag gcatggcgct
    18961 acacgctcat ctccggcgtc attccagccc tgccgctcat attgatccgt cccttcctgc
    19021 ccgagtcgcc cgaatgggcg cgcaagaaat ccgagggcac gctcaagcgc gccagcatcc
    19081 gcgagctttt cgcccccgag ctggtgcgca ccaccgtttt gaccaccctg gtcttcgccg
    19141 ccagctacgg catcgccttt ggcgccatcc agcagcttcc gcaaatcctc ggcgcgcagg
    19201 cgctgaacac cccgaatgag gccggccacg ccgccatcat cggcaccgcc aaggccgccg
    19261 tcggcaaagc cgtccaggaa gccgccgccg ccggcaaacc cgaaatcccc gccggcaaaa
    19321 agcgccagat cggcggcaat gccagtgatg aggccgtggc caaaatcacc ttctggcagg
    19381 agatcggcgg catgatgggg cgcctcgccc tcgccatcct cacggtcatg atcgtcagcc
    19441 gccgcgcgct cttccgtgtg ttccaaatcc cctccttgtt tttcgtgcct ctcctgttct
    19501 ggtggatttc cggccagctt gccaatcccg acagcctcgg cctcatcaaa atcggcatct
    19561 tcgtcgccgg tttcctcgtc gttgcccagt tcagcttctg gggcaattac atcccgctcg
    19621 tcttccccat gcacctgcgc ggcaccgggg aaagcttcgc cgccaacatc ggcgggcgca
    19681 tcctgggcac cgccgccgcc tggatcaccc tcaccctcgc cgcctcggac aagcccgacc
    19741 ccgcgcgcat cgccctcgtc ggcgcctgcg tggccggcgg ttacgcgctc atcggcgcca
    19801 tcctcaccca gttcctgccc gagccggagg ccgagcatca ggtctgaaac caagtaaaca
    19861 gcgccatgcc cgtgcccgtc ctctacgtca aaaccggctg cccctggtgt gacgaggtac
    19921 tcgacttcct cgacgagcgc cgcctgccct accgcaaagt cgtcgtctcc ggcaatgccg
    19981 ccgccatgcg cgaaatggcg gacctttccg gccaaaccaa agcccccacc atggactggg
    20041 atggcgaggt gctggccgac ttcggcgtgg acgaactgat ccctttcttg aaagcgcgaa
    20101 atgccctgtg aggggtattt ttcgatttat tcggtatttt tcgcgaatta ttcagcgggg
    20161 cgaaaaattt gccaaaaatc gcttttttcg gtccagtaat tcccttgtca ccgagttatt
    20221 cacaatcggc tgaaaatgaa gggaatcgca aaaagctgcg aaaattctca ttttcaggtg
    20281 tcatccccgc cattttgacg ccgaaaaaat ataattggcg tggaacgctt gaagctgcgt
    20341 tgtgcaacgc ctgacccgcc actgcggaat caaaaacatc ttggccggac acaagatatt
    20401 gtgcatcatc tcccccggcc cgcgcaatat ctggtggatt ttgtcaaggc cgtcactcct
    20461 tccgaaatcc tgcaaattca agccttccag cccttcaaac gccctcaatc ctcccgccct
    20521 tatggaaaaa atctacaaaa tcggcaatcg cgagttcccc ctcgaccagt ccaaggcaga
    20581 agcagcctac gcggcgaaaa aggtcatcaa tggccgccag tccatgacct tcaacctgct
    20641 tcccctcaag tatcagtggg cctatgacct ctaccgcacc atgaaggcca accactggga
    20701 gcccgaggac atccagatgc aaaaagacgt ggagcaatgg cgcggccacg agatttccca
    20761 gaacgagcgc tggatcatca tgatgggcat cggctacttc agcgccgccg agggcatcgt
    20821 cggggacaat atccagcacg tcgtacgcga gctggtcacc gcccctgagc tgaagctcgt
    20881 cctcggccgc cacgcccacg aggagaacat ccacgccgac tccctcctct acatgatctc
    20941 ctccctgggc atcaacccgc atgaatgcga ggccatgttc gagcagatcc ccaccattgt
    21001 caggaaaaac gagttcgtca cccggcactc gaacaacctc cgccgcgacc tcgacctcac
    21061 caagctcgaa aacaagcagc tcctcgccaa aaacatcttc gtcttcggcc agtgcatgga
    21121 gggcacccag ttctacggac tctttggcat gatcctcagc ctctaccgcc agaacaaatt
    21181 ccccggcatc ggccagatgt tccgttacac cctgcgcgac gaaagcaacc acatcgaagt
    21241 cttccgcaac ctcttcatgg acctcgtcga agaaaacccc gacctctgga ccgccgagtt
    21301 ccgcgaggag ctcgtccaga tcatgcgcga ggccatcgcc ctggaaaaag agttcatccg
    21361 cgactgcctg cccgtgaacg ccgtcggcct cagtgccggg gagttcgagc gctacattga
    21421 ctacatcgcc gaccgccgcc tcgccggctg cggcctccag gtcctcaacc ccggcgtgca
    21481 gaatcccctg ccctggctcg ccgagatgat ggatgtgcgc aaagagcaga acttcttcga
    21541 aggcaaggtc acggactacc agaaggcgtc cgccctcgtc cacacctccg acgacgatct
    21601 ctgaatcgtc acctcccccc tggggtggaa aatccgggcc ggactcgtcc agccagggtt
    21661 ttccatcctc caaaatctct cgcttctctt aaccaacctc attcccgcac cctcccgcca
    21721 tgctccagaa cctgctccac gaagacaagg ccctcaaaaa agtcgcccac acccccaaag
    21781 accagaaacc ccgctttgaa tggagcgcca tcgccgcggg cgtcaccggc tccgcaccca
    21841 ccgccatcaa agtcaaagtc ggcggagacg agcgtgactt cgacatgggc gagatcgccg
    21901 acaccatcgg cagcgccctc accgacctcc tgctcgcccg ccagaacgac caggacattt
    21961 acaacgacca aaaccgccgc ctcgtcctca ccatcctcac cgccgtcctc gaggaaatcc
    22021 agcagcaggc cggcgcccag gccggcgccc aggccggcgc caacggcggc gccaccttcg
    22081 ccgccaggga catctaccag tgcatcgagc gcgccctcgt gcgccacagc gcccacgaca
    22141 tcgcccgctc cctcgccgag cgccgcaaac gcgccgaata cgacagcctc gccgacaaca
    22201 ccctcccgca gcccctcatc gtgaacacca aagtcatccg ccgcagcggc cagctcgtcc
    22261 cctggaacca caacaaaatc gagatcgccg tccgcaaagc cttcctctcc ctcgagctcg
    22321 actccacccc cgccgtccag atcgccgaag ccgtcagcgg cgccgtcgcc gccgaaaaca
    22381 agcagttcat gcacatcgag gacgtgcaga acctcgtcga ggaggagctc atgaagcagg
    22441 gctacttcaa agtcgcccgc tcctacatcc agtaccgcgc cctgcgcgga aaaatgcgcg
    22501 aggccgagga gcaggaggcc gccgggcaga acgacatcga aagccaggac cagcagtccc
    22561 tcatcgtcgt caagaccagc gacggcggct ccttcctctg ggacggccag gacctcaagc
    22621 gccgcataga cttcgccatg ctcggcctcg acctctgcct cacccgcgcc gagatcgaga
    22681 tggagctgcg ccgctccctg aactccgaca tcaccctcga ccatctcaaa aagaccgtca
    22741 ttctcaacgc caagaccctc atgcagaagg acgccgactt cgccaagttc gccgcccgcg
    22801 tcctcctctc ctacatctat gaggaagtcc tcggctggga catcgtccgc gacggtatag
    22861 accagctccg cgagttccac cgccgcgcct tccgccgcaa cctcgcccgc ggcatcgaga
    22921 ttgaccgcta caacccccgc ctcctccagt tcgacctcga caagctcgcc gacgccctcg
    22981 accccgccgc cgacctcgac ttcgacttcc tcggcatcca gaccctctac gaccgctacc
    23041 tcatcgtgga caaaaaggtc aagccctcca ggcggctcga aaccccccag ctcttctgga
    23101 tgcgcgtcgc catgggcctc tgcgtccagg aggacagccc cgaggaaaaa atcatctccc
    23161 tctacaagct ctacaaaggc cgccgcttct gctcctccac ccccaccctc ttcaacagcg
    23221 gcacccacca ctcccagctc agctcctgct acctctacaa agtggacgac agcatcgagt
    23281 ccatcatgat ccgcggcatc gccgaaaacg ccttcctctc caaatgggcc ggcggcctcg
    23341 gcggctcctg gaccagcgtc cgcggcaccg gcggctacat caaaggcacc aacggcgagt
    23401 cccagggcgt catccccttc ctcaagctcc acaacgacca gctcatcgcc gtcaaccagg
    23461 gcggcaagcg ccgcggctcc ggctgcgcct acctcgaagt ctggcacaac gacatcgagg
    23521 acttcctcca gctccgcgtg aacaccggcg acgagcgccg ccgcacccac gacctcaaca
    23581 ccgccaactg gatccccgac ctcttcatga agcgcgtcga gtcccgccag ccctggaccc
    23641 tctttcgcgc caacgaagtc cccgacctcc acgacctcta cggcagcgcc ttcgaaaagc
    23701 gctacgagga atacgaggcc ctcgccaaag ccggcaaaat cttcggcaaa gagacccccg
    23761 ccattgacct ctggaagctc atgctcaagg ccctcttcga gaccggccac ccctggatca
    23821 ccttcaaaga cccctgcaac ctccgcagcc cccaggacca caccggcgtc atccacagca
    23881 gcaacctctg caccgagatc accctcaaca cctccgccga cgagaccgcc gtctgcaacc
    23941 tcggctccgt gctcattgac aaccacctcg acgacgccgg cggcatggac cacaccaagc
    24001 tccgcgagac catccgcacc gccgtgcgca tgctcgacaa cgtcatagac atcaacttct
    24061 accccaccga ggccgcccgc accgccaact cccgccaccg ccccatcggc ctcggcgtca
    24121 tgggcctgca atacgccctc taccgcaaag gcatcccctt cgcctccagg gaagccgtcg
    24181 agttcaacga cgaattcatg gaagccgtcg cctactacgc ctatgaggcc agcagcgacc
    24241 tcgcc
//
LOCUS       DAOMYJ010000058        24021 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Prosthecobacter sp. isolate AMR_MDS_5629
            NODE_754_length_24021_cov_22.190228, whole genome shotgun sequence.
ACCESSION   DAOMYJ010000058 DAOMYJ010000000
VERSION     DAOMYJ010000058.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867850
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prosthecobacter sp. (wastewater metagenome)
  ORGANISM  Prosthecobacter sp.
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Verrucomicrobiaceae; Prosthecobacter.
REFERENCE   1  (bases 1 to 24021)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 24021)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.30
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:34:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,742
            CDSs (total)                      :: 4,684
            Genes (coding)                    :: 4,676
            CDSs (with protein)               :: 4,676
            Genes (RNA)                       :: 58
            tRNAs                             :: 55
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24021
                     /organism="Prosthecobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_754_length_24021_cov_22.190228"
                     /isolate="AMR_MDS_5629"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1965333"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(337..1851)
                     /locus_tag="PLP58_08205"
     CDS             complement(337..1851)
                     /locus_tag="PLP58_08205"
                     /inference="COORDINATES: protein motif:HMM:TIGR03025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0032508 - DNA duplex unwinding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exopolysaccharide biosynthesis polyprenyl
                     glycosylphosphotransferase"
                     /protein_id="HRJ08534.1"
                     /translation="MPVPKSQPSSGDPATLIGLPGPMTGNLLEKLEERRAQRAQEQPS
                     PHLWVLVSILGDLVMALLSGYAAYWLRFHSMREFGNWDSITLRQYGGHITLGTLSLVL
                     AIGWQGIYHRNVLLRSRWIASKLAKAVLIWTLCFLAVNLAFKMEPPISRFYVALNGAC
                     ALLLLLGWRSAFNAFLHTRKRAAALQQRTLFVGWNDDAQRLWQTLSTDTAHAFDLVGW
                     VDASGWRDKKPPPTELPFLGYIEEMHRIVARHAVDMVIVADLPRQHLIELANLCEREM
                     IQFKLAPSVFRIFVSGLSLETIAGMPVLGINRLPLDSTVNVLAKRALDICGALAGLVL
                     SAPLVAVFGALVWLESRGPVFYRQRRWGQNGVPFEIVKIRSMKLDAEAGAGAQWCQEN
                     DPRRLRVGAFMRRWNIDEVPQFWNVLKGEMSLVGPRPERPELISGFKHEIPHYNARHH
                     AKPGMTGWAQIKGLRGDTDLAERIKCDLWYLENWSLWLDLQIMFLTFFKRDNAY"
     gene            complement(1966..3012)
                     /locus_tag="PLP58_08210"
     CDS             complement(1966..3012)
                     /locus_tag="PLP58_08210"
                     /inference="COORDINATES: protein motif:HMM:NF013595.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ08535.1"
                     /translation="MRINTLLALLSLLVSARADRIVLVAGGARDATGVPALEAALKEP
                     FGTEFDTAGNTWIVEMVSGNRLLKVDASGLLTHAAGQLKAGFSGDGGPALQAQFNGPH
                     NLAVLPGGDILIGDTWNGRVRKVDVKTGIVSTLPGWQAPEGRERGNGPYCITLSPDKT
                     KLHIADLRQVHELDLVTGKSRVVAGNGKKGVPEDGALAVEAPLADPRAAAMDSQGNLY
                     VLERNGNALRVVGRDGRIRTVVNASGKKGGDGDGGSALDASMSGPKHLCVDRDDSVLI
                     ADAESHLIRRYVPATGRIERIAGTGKKGAAGVGGDPLQCELARPHGVTIHPRTGELFI
                     TDSYNNRVLKIVRE"
     gene            complement(3091..4233)
                     /locus_tag="PLP58_08215"
     CDS             complement(3091..4233)
                     /locus_tag="PLP58_08215"
                     /inference="COORDINATES: protein motif:HMM:NF012488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase family protein"
                     /protein_id="HRJ08536.1"
                     /translation="MTRYFDHNATTPLSPAARSAWLQAEDRHWHNPSSLYREAGLAAH
                     ALDAAREELAGIIDTEAARIVFNSGATESNNTLFAGLARCLPPDASVAISAIEHPSVR
                     QAAQAWLGRARVLEIAVDGQGIVQPDDLRAFLQRHKPALVSIMAASNESGVLQPWPDL
                     LAICRGHGARFHCDAAQWFGKLPAAGLGACDYLTGSAHKFGGPKGAGFLMLADEQESF
                     PLLHGGPQETGRRAGTENYPAIAAMLAALRESEAEIPRAMSAQPLLRDAFFARLQSAF
                     PGLRLISDTAPRLWNTLLMVMPRHSNLKWLARLSRRGFAISTGSACSSGREGSSVVVQ
                     ALGADWEELRRVVRVSGGRHATAAGWQALAEAFEETARELDEGGRP"
     gene            complement(4259..5962)
                     /locus_tag="PLP58_08220"
     CDS             complement(4259..5962)
                     /locus_tag="PLP58_08220"
                     /inference="COORDINATES: protein motif:HMM:NF012320.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0000917 - division septum assembly
                     [Evidence IEA];
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HRJ08537.1"
                     /translation="MVEYDRSLLRDESGSPALRTCIVGVGGAGGNVIDRITLDRTVDA
                     KLVAMHTDVRVLSHSMAPVKIQLGADLMRGVGAGGDPDLGREAAMFSREEIRQEIDGH
                     DIVFICAGLGGGTGSGAAPVVAEIAKSTGALVLVTATMPFDFEGRRRLAQAEEALAQL
                     QKRADALVLFENNRMCELILPKDGIQKAFAQADQLIAQSLRAVSTIVTLPGLVKLGLD
                     DLTSALSTSDGRCLFGFGEARGQHRGAEALKRALKSPLIDQGRLLHQTRTLLVHIAGG
                     ESLTLVEVDTIMKQLGRHVPDHTHILFGVAVDARLADSVSVTLVSSLGLAQLNTVAAA
                     APPAEMLPLTDRPMPSVAAAVIPGLKSKVIAPPAPKEVKKQPPPPAASVPAPAPAPAA
                     DPSLDLIFTDDEIIVHHQPEDASSPVTQDEFELEAPSSSHDLPPAQDEVEFEHEDDYD
                     VTQETPSDSAADPEPEPAPEQPAPEPEKPRRLRIEDFMTPPPEPDPVPPQTPVIARRP
                     LTPLAAVKKSPPPGPAQIDLGLGADEEPARFKGTEPAIAPGGENLDVPTWKRLKMKLK
                     R"
     gene            complement(6012..7229)
                     /gene="ftsA"
                     /locus_tag="PLP58_08225"
     CDS             complement(6012..7229)
                     /gene="ftsA"
                     /locus_tag="PLP58_08225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009962556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HRJ08538.1"
                     /translation="MAKSTIYVALEIGTSKICVVVGEAKRDGAIKILGVGQAPSRGVR
                     KGEIVDFEKAQTCLNDALVRAEDLSDVMVKTVFLGVSGAHIQSINNRGLHCLPEGRTE
                     ILEKDVEAAKKTARETVDVPEDHIFLHSVSRQYIVDGVEGVRLPVGRQGRQIEADYLI
                     IHGQMPRIQNSIKCVKEMPLEVEDIVFNPVAAAQVVLSREAKREGALMIDIGGGTADF
                     VLYDDSMLTTAGCVPLGGDHISQDIAQALHIYHARAESLKINTGSCIVDDAATGEIVR
                     IEDDNGYAIGEVERAFLNEIIQLRVREIFEQVRLRCEGHLDRLAAGVFLTGGSSLLRG
                     IDIVARDVFGMKVTRAGSAPVSGVTAMFEKPQFSAPIGLIRYAQILDQEKPPFSPFRF
                     LKRKMSDILMPAA"
     gene            complement(7236..8237)
                     /locus_tag="PLP58_08230"
     CDS             complement(7236..8237)
                     /locus_tag="PLP58_08230"
                     /inference="COORDINATES: protein motif:HMM:NF015739.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsQ/DivIB"
                     /protein_id="HRJ08539.1"
                     /translation="MPSSRPQAPVRQNPASKRGAALVDEKNVRKSARKPRIIYAAELP
                     VDSPKIRQMARQSATRLGFKLATGLIITICVLGLLKVTVQETILKNPQFALVQIAVDT
                     SGPLSVDKIARTTLLTHGENLLFINMREVRARLRQLPAVTGVALERDFDKGVLTLRVA
                     QRRPVAWLACPRLGMAEGRAEGCWLVDSEGATFPCDYQPDNYAALPVIHHERLQQNTP
                     GLALDDLSLRSALHLVKELDRRHEDGQPQARAMHIQTPWSILVQFSDKTQVIFGVDEL
                     ERQLARLDQFWFHARQRRWDVDTLNLAVSKNVPVTFRSPPDLAGLGMPAANAATTTR"
     gene            complement(8403..9323)
                     /locus_tag="PLP58_08235"
     CDS             complement(8403..9323)
                     /locus_tag="PLP58_08235"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009962559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HRJ08540.1"
                     /translation="MLDLSSQRIAVLFGGPGSEREVSVKTAESVGQALESLGAEVLPV
                     DVRGPDFEYPADVLIAMNLIHGTFGEDGQIQAILERRGIRYTGAGVESSRVAFDKALS
                     KEKFLAAGVPTPRSQIWPLDGGVPLEIQPPLVSKPPREGSSVGVKIVRDASEWAAAVE
                     EARRHGDATLVEEFIEGRELTVGILGDLVLPVIHIEPVDGFYDMNNKYPWMTGKGRTL
                     YHCPADLDDAVTRRVQDAAMAAFRSCGTEVYGRVDVMLRADGQPFVLEINTIPGMTSS
                     SLLPKAALAAGISFSELCARIIELSLQARP"
     gene            complement(9417..10274)
                     /locus_tag="PLP58_08240"
     CDS             complement(9417..10274)
                     /locus_tag="PLP58_08240"
                     /inference="COORDINATES: protein motif:HMM:NF014376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HRJ08541.1"
                     /translation="MSQPAKETVFLRNYHATPLEWQRDLFFCVMRAGHLRGGAGHRIG
                     RETYPGHELILCLGGRGWVQVAGRRHEAGPGDLVWVNCHHPHLYGAVKQDPWDLYWVR
                     VEGRTLDRLGELLEVRSRPVFAGVEPEAAKGDFEGIFHRLESGRPCDAAHVNARVAAL
                     LAAAFESRLGDALELRPELPQPVQRALERMRLYFHEPIRVAELAALAGMSESHFSRQF
                     KAFVGTSPIDWLRRERINQAKRRLAESDDAVKEIARQAGYTDQFFFSKDFKRMTGLTP
                     SQFREQERH"
     gene            10431..10925
                     /locus_tag="PLP58_08245"
     CDS             10431..10925
                     /locus_tag="PLP58_08245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018969394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin domain-containing protein"
                     /protein_id="HRJ08542.1"
                     /translation="MSLSTGSPAPDFTLATLGANGPELFKLSENLGQANLLLLFVPMA
                     FTGVCTKEFCEISNGINEFTKLNARVVGISGDNPFAQKAWAEKENITLQLLSDYDHHV
                     ATAYGVAYQSFLPAKNLIMGGVPKRSAFIVDKAGIIQYAEVLESPGDLPNFDAIKSKL
                     AELA"
     gene            11071..11724
                     /gene="pdxH"
                     /locus_tag="PLP58_08250"
     CDS             11071..11724
                     /gene="pdxH"
                     /locus_tag="PLP58_08250"
                     /EC_number="1.4.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009962718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004733 - pyridoxamine-phosphate oxidase
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase"
                     /protein_id="HRJ08543.1"
                     /translation="MMDFPPDTLRDLRVSYDLDNLVEESAPADPRVLFQAWLKDALEH
                     QLPEPNAMTLATIGEDGGPSARTVLLKGLDSKGFHFFTNYESRKAREIAANPRASAVF
                     LWTQRHHQVCVRGFIKKLPREETESYFATRPAGHQIGAWVSEQSRVIPGRAWLEAREA
                     EMRARFGEGPIPCPPHWGGYALVPDEFEFWQGRVSRLHDRLRYTREGAGWKIERLSP"
     gene            11763..12815
                     /locus_tag="PLP58_08255"
     CDS             11763..12815
                     /locus_tag="PLP58_08255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ08544.1"
                     /translation="MKRRRFLSTTAASTLSAPFVRAQSKTSLNGATIGHGAHRYQVDL
                     EWCKADRAAHPVKNCHEMVMDSQKRLIMITDEARNNILVFDKEGKVLDAWTLNLRSGH
                     GLSLDATGGKEHLILCDPSSGTVVKTTLDGKEVMRLPHARDCGAYDAAMKYAPTEAVT
                     APNGDFYVADGYGSQFILHFDARGKFIRKFGGQSTQAMNPGKFMQAHGVAMDLRGPEP
                     LVLCTERVRNEFHWYSLDGIYLRTVYLPGAFMSRPVINGDLLLSGVCFGMKPGDYRMW
                     RERGFIIILDRENRVVSVPGGHEPVHDDKGQVALLLQNEPVFRNVHDVCVDDAGDLYA
                     CQWNADQAYPFKLRRV"
     gene            complement(12846..13796)
                     /locus_tag="PLP58_08260"
     CDS             complement(12846..13796)
                     /locus_tag="PLP58_08260"
                     /inference="COORDINATES: protein motif:HMM:NF014477.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HRJ08545.1"
                     /translation="MPVSNRLLPLLLCLPLSGRALERFDFSGPGMATTFRIACHAETR
                     DVAERAARECFERVAFLHAICTDYDPASELMRLCAPGAVHPFQTSAELYDILLKAREL
                     AVLTQGAFNPACGHLTQLWRRTRRTGKLPPEDRLQRGLKAADWQRMSFEPETRGVTLA
                     EGTLLDLGGIAKGWAADECLRLLKRHGITRAVVQAGGDTAVGSPPPGEEGWEIKLRTF
                     SKPGGDDELRALVLAECAVSTSGDLYQFTEIGGVRYSHIVSPFTGMGLTERVACSVIA
                     PDCATSDALATAMCVLGEQRGTALAAGLPGVRVIFAGEKK"
     gene            complement(13833..14840)
                     /locus_tag="PLP58_08265"
     CDS             complement(13833..14840)
                     /locus_tag="PLP58_08265"
                     /inference="COORDINATES: protein motif:HMM:NF012705.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="squalene/phytoene synthase family protein"
                     /protein_id="HRJ08546.1"
                     /translation="MSDESRHERELGGQLLASVSRSFYLTLKALPAGLREPLSLAYLL
                     ARTADTLADTVTVPAATRLECLRLYESCAQGAASASESGELSARIAREFAPLQSDAAE
                     RRLMERFAGALAWLGIMRPGPLRAIRGVLHHIIHGQMLDIERFPADGRLRSLPDAAAL
                     EEYTWLVAGCVGEFWTDMCATESPGALEADMSLDEMRKWGASFGKGLQLVNILRDVGE
                     DLRDGRCYLPGVLKDGSADVAALRAAWDHWAGICEKHLESGLPYLRHVADGKLRYATA
                     LPLLLAARTLAKMRAASWDEVLRGVKVSRLDVAMILAEAAVSCRQPDKLERLYRKLGA
                     V"
     gene            complement(14833..15843)
                     /locus_tag="PLP58_08270"
     CDS             complement(14833..15843)
                     /locus_tag="PLP58_08270"
                     /inference="COORDINATES: protein motif:HMM:NF024989.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent alcohol dehydrogenase"
                     /protein_id="HRJ08547.1"
                     /translation="MKAYRITGTEGLASLRRVELPDPFPKPGEVLVRINSVSLNYRDH
                     MNVTGLHGITGPVPRIPCSDGAGEVVSTGFGVTGWKPRDRVVVPFMPAWLDGDFTEMH
                     AAQALGGACDGMLRELVCLRAESLVRLPGHFSFDEGSTLPCAAVTAWDALHVRGRVRA
                     GETVLIQGTGGVSIFALQFARQAGARVLAITSSDEKAARLLELGAEKVHNYKTDPAWD
                     AWAQRQTEKRGVDKVVEIGGPQTLNRSINAARFGGHIALIGVLTGTAGDVRTVGILRK
                     GIRLDGVYVGSRSMLVEMLGVMTAAGLRPVIDSTFEFDQAHEAFRRLESGAHFGKIVI
                     HV"
     gene            complement(15990..19523)
                     /locus_tag="PLP58_08275"
     CDS             complement(15990..19523)
                     /locus_tag="PLP58_08275"
                     /inference="COORDINATES: protein motif:HMM:TIGR02604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NPCBM/NEW2 domain-containing protein"
                     /protein_id="HRJ08548.1"
                     /translation="MRLALTLLLPTLALAAPKPLFRSPVIDTKTPNHSVDIDVPLEGA
                     KELFLVVTDAGNGFTADWADWAEPRLVLGDGSEKKLTEIEWKSAQSGWGGVRVNANAQ
                     GQELRSAGKAIEYGIGTHANSVIAFNLPAGVKRFKARGALDEGGTKQGNSTSVTFSVF
                     TENPGPIAASDNNAASHEPEAATEQLDVHPECLVQLFASEPMMLSPASIDIDHRGRVW
                     VCEVVNYRKHNGERAEGDRILILEDTDGDAKADKSTVFHQGPDVNSAHGICVLGNKAL
                     ISCGDDVFWLIDDNGDDKADRKEFLFTKIGGAQHDHGIHAFHFGPDGRLYFNFGNAGK
                     QLCDKNGNLITDIHGVKCTNQNNRPYQQGMVFRCELDGSGVEVLAWNFRNNWEVAVDS
                     FGTMWQSDNDDDGNKGVRINYVMEFGNYGYADEMTGAGWQTPRIGWESEIPQRHWHQN
                     DPGVVPNMLMTGAGSPTGMQVYEGDLLPSIFHGQPLHCDAGPNVVRGYITQPDGAGYK
                     AEIVNLLDGTHNRWFRPSDVAVAPDGSLIVADWYDPGVGGHGMGDVTRGRLFRVTTKA
                     AEKFQVPSFKLETVEGAIDALKSPNEAARYLAWEALKKLGTKAEPTLKQLFDDKKAEP
                     QHRARALWAIGEGEFKKPAIVSRKAEEKNTFSLSNALKDSDMNIRLTAVRLARRLWQK
                     DGVLYRAEREKLREALGIAASDTSPAVRREVAIALRFDGTDLGSGLWCSIGEQCATSD
                     RWMIEALGIGEDLQGDSRFYPFHGKAKGNTESATRAELIRRHRSSLTPMVIASMFINS
                     IDEHSTWAKNNSLHFLRALQLLSYHHREEVAKAALTIFLKADAETALASTALLDRNAI
                     ASNPEAKARLDSLLKPVVGKPEFVALVEKLNLTGFEKELLDFISANPNAPESVNAARL
                     IAANRDGALRFLKDADAKSAKALIAALGRVSDRAAVAVMNQHFWPEKREDVRRVLIDA
                     LALSGEGGRAILKWQSEGKLPPQFKDQAALALSRSTDAGLRDQASRELPLPAAQGLAN
                     FPKLPELLKLKGDATKGQAMFMQAGCVACHRVKGQFIDFGPDLTQIGAKLSQDGLFTA
                     ILYPSAAIEHSYVGLTVTTKDGQTVVGYVVSETDAELTLRMAGGASTTVKKVNVVKKE
                     ELKDSLMPPGLVGAIGPQGLADLVAWMMTLR"
     gene            complement(19620..21200)
                     /locus_tag="PLP58_08280"
     CDS             complement(19620..21200)
                     /locus_tag="PLP58_08280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009959157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HRJ08549.1"
                     /translation="MMHRLSAATACALLLAACGETLPRADLVFIQSAEPETLDPALAT
                     DQVSMRVSAALFEGLCRVNEHGRPEPGMALRWEISEDRKTYTFHLRPGAAWSDGTPVT
                     AQDFLRSWRRALTPDTGADYASLLHIIRGARAFTEQGAAWDQVGLHAPDERTLVVELE
                     NPVPYFIDLCSFATFAPVPLRVIEEKGTAWIKPGCIVTNGPYLLEDWRLDDRIRLRKN
                     PAYWDAENVRMTTVEVRPSQDAGTALACFHTGGCDLLMDKGMVPPTLTQKLKQQPWFH
                     TGPFLGTWFIRINNTRPPFDDPLVRQAFALAVDKKRIVEKITRLGESTARGIVPPGTG
                     RDYQPPPGLDHDPARARQLLAEAGYPGGKGFPRVEYLYIPLAVERNIAVELQAMWQDA
                     LGVTVNLAKQEQKVWLKSMRELDYHLCRSSWVGDYNDPSTFLDLFQSGNGQNRTGFAS
                     PEYDRLIRAAAAEPDPAARNRVFQQAEQHLITDRAALIPVYYYVGVQFYDASRLTGVR
                     ANLIDDHPFRCMAWRLPP"
     gene            complement(21318..23840)
                     /locus_tag="PLP58_08285"
     CDS             complement(21318..23840)
                     /locus_tag="PLP58_08285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007418546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HRJ08550.1"
                     /translation="MNNFTPRAQQVLALARKEADRFNHNYVGTEHLLLGLIKLGQGVA
                     VNVLTKLGLDLETVRLHVEQQVGSGPETKMVGNIPYTPRVKKVLALASKEAKALNHSY
                     VGTEHILLGLLREGEGVAAQVLRTLDVNLDKARNEILRELDPNFSGGAAGEDDDDAEN
                     VTPGGSVDEEKKKKKNEKTPALRAFGRDLTEVAQKGEMDPVIGRADEIARVIQILCRR
                     TKNNPVLIGEAGVGKTAIVEGLAQEIAVGNVPEILRDKRVITLDLALMVAGTKYRGQF
                     EERIKAVMDEIRRNKNVILFIDEMHTIVGAGSAEGTMDASNIIKPALSRGELQVIGAT
                     TLNEYRKYIEKDSALERRFQQVKVEEPSIEDAIKILMGLKSKYEMHHKANYSDEAIRT
                     AVNLSARYLTARFLPDKAIDIMDEAGAKARIAAMTRPPQLKDIEEEIESIRVEKETSI
                     KDQDFEKAARLRDKERKAKNRYDELFDTWRKNNKEQIVDVSEDDIMSVVSKWTGVPLQ
                     RMEQAEAAKLLKMEQELKGKVIGQDEAVAAISKALRRSRADLKDPRRPIGSFLFLGPT
                     GVGKTFLAKNLAEFMFGSAEALIQLDMSEYMEKHTASRLIGAPPGYVGYEEGGQLSEA
                     VRRRPYSVVLFDEIEKAHPDVMHLLLQILEEGQITDNFGRKIDFRNTIVILTSNVGAE
                     QIKRQTSLGFAAMAEGEADNAGIKGKLGEAAKKFFKPEFLNRLDDLIVFRMLEKTELR
                     VIVDLEIAKVVSRIKGKNIILEMEESAVEFLMKEGYDPQYGARPMRRAVEKHIEDPLA
                     EHLLRGDVREGDRVRVSFDEEAKRLKFAAEKSAAPAETTPAP"
BASE COUNT         4218 a         7651 c         7729 g         4423 t
ORIGIN      
        1 tggcggaggg attgccaaaa cgcgcgccca gccacggcag catggcctcc acaacctccg
       61 ggtcaggcgg agtggtggcg ttggcgtcga ggtagatcac cctgggagat aaggtggaaa
      121 atcgaaattc gaaattcgaa attcgcccct tcccttaacc gtaacgatgc agttgaacca
      181 cgaatggaca cgaatggaca cgaatccatg cggaggagag agggatgggg gcatcaccca
      241 ggggtgatct cccggcgctt cagctcaccg gacatgatgc ctccaaaatt cgtgctcatt
      301 cgtgtccttc gtggttttcc agcgcatgaa cacggcctaa taagcgttgt ccctcttgaa
      361 gaaggtgagg aacatgatct ggaggtcgag ccagaggctc cagttttcga ggtaccaaag
      421 atcgcacttg atgcgctcag ccaggtcggt gtcgccgcgc aggcccttga tctgcgccca
      481 gccggtcatg ccgggtttgg cgtggtggcg ggcgttgtag tgggggattt cgtgcttgaa
      541 gccggagatc agctccgggc gctccgggcg cgggccgacg aggctcatct cgcctttgag
      601 cacattccaa aactgcggca cctcgtcaat gttccagcgg cgcatgaaag cgcccacgcg
      661 caggcgacgc gggtcgtttt cctggcacca ctgcgcgccg gcgccggcct cggcatcgag
      721 cttcatggag cggattttga cgatttcaaa gggcacgccg ttctgccccc agcggcgctg
      781 gcggtaaaac acggggccgc gcgactccag ccagaccagc gcgccaaaga cggcgaccag
      841 cggcgcgctg agcacaaggc ccgccagggc gccgcagatg tccagcgcgc gcttggcgag
      901 cacgttgacc gtgctgtcga gcggcaggcg gttgatgccc agcacgggca ttccggcgat
      961 ggtctccagc gagaggccgg agacgaagat gcggaagacg gagggcgcga gcttgaactg
     1021 gatcatctcc cgctcgcaca ggttcgccag ctcgatgagg tgctggcggg gcagatcagc
     1081 gacgatgacc atgtccacgg catgacgcgc cacgatgcgg tgcatctcct cgatgtagcc
     1141 gaggaagggc agctcggtgg gcggcggttt tttgtcacgc cagccggagg cgtccaccca
     1201 gccgacgagg tcgaaggcgt gggccgtgtc ggtgctgagg gtctgccaca ggcgctgggc
     1261 gtcgtcattc cagccgacaa aaagcgtgcg ctgctggagc gcggcggcgc gtttgcgcgt
     1321 atgcaggaag gcgttgaagg ccgagcgcca gcccagcagc agcagcaggg cgcaggcgcc
     1381 gttgagcgcc acatagaagc gcgagatggg cggctccatt ttgaaggcca ggttcaccgc
     1441 caggaaacag agcgtccaga tgagcacggc cttggccagc ttggaggcga tccagcggct
     1501 gcgcagcagg acgttgcggt gatagatgcc ctgccagccg atcgccagca cgaggctcag
     1561 cgtgccgaga gtaatgtgcc ccccgtattg ccgcagcgtg atgctgtccc agttgccgaa
     1621 ctcgcgcatt gagtggaagc gcagccagta agccgcgtag ccggaaagca gggccatgac
     1681 caaatctcca aggatcgaga ccagcaccca gagatgcggc gaaggctgct cctgggcgcg
     1741 ctgggcgcgg cgctcttcca acttttccag caaattccca gtcatcggac cgggcaggcc
     1801 gatgagtgtg gcagggtcgc cagacgaagg ttgggatttg ggaacgggca tggggtttat
     1861 tatagaaatt ttaaaatata gctgtaattg cccctttcct gcaactgata gccagcaaat
     1921 cctgattatt ttccagaaat tttaatccct cccttctctg actcttcact ctctgacgat
     1981 cttcagcacg cgattgttgt agctgtcagt gatgaacagc tcgcccgtcc gcgggtggat
     2041 ggtcacgcca tgcgggcggg cgagttcgca ttgcagcggg tcgccgccga ctcccgcagc
     2101 gccctttttc cctgtgccgg cgatgcgctc gatcctgccg gtggcgggga cataacggcg
     2161 gatgaggtgg ctttccgcgt cggcgatgag cacgctgtcg tcccggtcca cacacagatg
     2221 cttggggccg ctcatgctgg catccagggc ggagccgcca tcgccatcgc cgcctttttt
     2281 gccggaggcg ttgacgacgg tgcggatgcg cccgtccctg ccgacgacgc gcagggcgtt
     2341 gccgttgcgc tccagcacat aaagattgcc ctggctgtcc atggcggcgg cgcgcgggtc
     2401 cgcgagcggg gcctcgaccg ccagcgcgcc gtcctcaggc acgccttttt tgccattacc
     2461 cgccaccacg cgggacttgc cggtgacgag gtcgagttca tgcacctggc gcaggtcggc
     2521 gatgtggagc tttgtcttgt cagggctgag ggtgatgcag taggggccgt tgccgcgttc
     2581 cctgccctcc ggcgcctgcc agcctggcag ggtggagaca atgccggttt tcacatccac
     2641 cttgcgcacg cggccgttcc aggtgtcgcc aatgaggatg tcgccgccgg gcaggacggc
     2701 gaggttgtgc ggaccgttga actgcgcctg caatgcgggg ccgccatcgc cgctgaatcc
     2761 cgccttgagc tgccctgccg catgggtgag caggccggag gcatccactt tcagcagcct
     2821 gttgccggag accatctcga cgatccaggt gttgcccgcc gtgtcgaatt ccgtgccaaa
     2881 aggctccttg agcgcggctt cgagagccgg gacgccggtg gcatcccgcg cgccgccggc
     2941 cacgaggacg atcctgtccg cgcgcgcgga caccagcagg gaaagaagag cgaggagggt
     3001 attgatgcgc atggcgatga gattggagag aacgattacg cggattgcag gcacccattg
     3061 cacgccgcct ggcatcgccg tgtcaagccc tcacggcctg ccgccctcgt cgagctcccg
     3121 cgcggtttcc tcgaaggctt cggccagcgc ctgccagccg gcggcggtgg catgcctgcc
     3181 gccgctgacg cggacgacgc ggcgcagttc ctcccagtcc gcgccgaggg cctggaccac
     3241 gaccgacgag ccttccctgc cggagctgca cgcggagcct gtggagatgg caaagccgcg
     3301 ccggctgagg cgggcgagcc atttcagatt ggaatggcgc ggcatgacca tgagcagggt
     3361 gttccagagg cgcggagccg tgtccgagat caggcgcagg ccgggaaagg cgctttgcag
     3421 gcgggcaaaa aaggcgtcgc gcaggagcgg ttgcgcgctc attgcccggg ggatttccgc
     3481 ctcggactcg cgcaaggcgg cgagcatggc ggcgatggcc gggtagtttt ccgtgccagc
     3541 gcgccgcccg gtctcctgcg gtccgccatg cagaaggggg aagctttcct gctcgtctgc
     3601 gagcatgaga aaacccgcgc ctttgggacc gccgaacttg tgagcgctgc cggtgagata
     3661 gtcgcacgcg ccaaggccgg cggcgggcag tttgccaaac cattgggcgg cgtcgcagtg
     3721 aaagcgcgcg ccatgcccgc ggcagatggc gagcaaatcg ggccagggct ggagcacgcc
     3781 gctttcgttg ctggcggcca tgatggagac gagcgcgggt ttgtgccgct ggaggaaggc
     3841 gcgcagatca tccggctgga cgatgccctg accgtccacg gcaatctcaa gcacacgcgc
     3901 gcggcccagc caggcttgag cggcctgacg gacgctgggg tgctcgatgg cggagatggc
     3961 gacagacgca tccggcggca ggcagcgggc gagtccggcg aagagggtgt tgttggactc
     4021 agtggcgccg gagttgaaga cgatccgcgc ggcctcggtg tcgataatgc cggccagctc
     4081 ctcgcgcgcg gcatccagag catgggcggc gaggccggcc tcgcggtaaa ggctggaggg
     4141 gttgtgccag tggcgatcct cagcctggag ccacgcggaa cgcgcggccg gtgagagcgg
     4201 cgtggtggcg ttgtggtcaa aatagcgcgt catgcccgtg gacgggacgc ctgccaggtc
     4261 agcgcttcaa tttcattttg agacgcttcc aggtgggcac gtcgaggttt tcgccgcccg
     4321 gagcgatggc aggctcggtg cctttgaagc gggccggctc ctcgtccgcg ccaaggccga
     4381 gatcaatctg ggccggacct ggaggcgggg attttttgac ggcagccagc ggtgtgagcg
     4441 gacggcgcgc aatgacgggc gtctgcgggg ggacgggatc aggctcagga ggcggtgtca
     4501 tgaagtcctc aatgcgcagg cggcgtggtt tttccggctc tggcgcgggc tgctccggcg
     4561 cgggttcggg ctcgggatcg gcggccgagt ccgacggagt ttcctgggta acgtcataat
     4621 catcctcgtg ctcgaactcc acctcatcct gcgcaggcgg caagtcatgg gacgaggatg
     4681 gcgcttccag ctcaaactca tcctgcgtca ctggcgacga ggcatcctcc ggctggtgat
     4741 ggacgatgat ttcatcgtcg gtgaagatca agtccaggga agggtcggct gcgggtgcgg
     4801 gtgcgggtgc ggggacggaa gcggcaggag gcggaggttg ttttttcacc tccttcggcg
     4861 cgggcggggc gatgactttg cttttgagtc cgggaatgac ggcggctgcg acgctgggca
     4921 tgggccggtc cgtgagaggc agcatttcag cgggcggggc ggcggcggcg accgtgttga
     4981 gctgggcgag gccgagagag ctgacgagag tcacggagac ggagtcggcg agacgggcgt
     5041 caacggcgac tccgaacaaa atgtgcgtgt ggtcggggac atggcggccg agctgcttca
     5101 tgatggtgtc cacctcgacg agcgtgagag actcgccgcc ggcgatgtgg acgagcagcg
     5161 tgcgggtctg gtgaagcagg cggccctggt caatgagcgg actcttgagg gcgcgcttga
     5221 gcgcctcggc gccgcgatgc tggccgcgcg cctcgccgaa tccgaagagg cagcgcccgt
     5281 cggaggtgct gagcgcgctg gtgaggtcat caaggccgag cttcaccagg ccgggcaggg
     5341 tgacgatggt ggagacggcg cgcaggctct gggcgatgag ctggtcggcc tgcgcgaagg
     5401 ctttctggat gccgtccttg ggcaggatga gctcgcacat gcggttgttc tcaaaaagca
     5461 cgagtgcgtc ggcccgcttt tgcagttgag cgagcgcctc ctcagcctgg gccagccggc
     5521 ggcggccttc gaagtcaaag ggcatggtgg cagtgacgag gacaagagcg ccggtgcttt
     5581 tggcgatctc ggcgacgaca ggagccgcgc ctgatcccgt gcctccgccc agccccgcgc
     5641 agatgaagac gatgtcatgc ccgtcaatct cctggcggat ctcctcgcgg gagaacatgg
     5701 ctgcctcgcg tccgagatcc gggtcgcccc cggctcccac gccacgcata agatcggcgc
     5761 cgagctgaat tttcaccggg gccatggaat gcgagagcac gcgcacatcc gtgtgcatgg
     5821 cgacgagctt ggcgtccacg gtgcggtcga gggtgatgcg gtcaatgaca ttgccgcccg
     5881 cgccgccgac tccgacaatg caggtgcgca gggcgggaga gccggattca tcccgcagga
     5941 gagagcgatc atattcaacc atggtgatgt gggggtggtg gagggtggtg cgccggagac
     6001 cggcgcgttg tctaggccgc aggcatgaga atgtccgaca ttttcctttt gaggaagcgg
     6061 aaaggtgaga agggcggttt ttcctggtcg aggatctgcg cgtagcggat gagcccgatg
     6121 ggagcggaaa actggggctt ttcaaacatg gcggtgacgc cgctgacagg cgcggacccc
     6181 gcgcgcgtga ccttcatgcc aaaaacatcc ctggccacga tgtcaatgcc ccgcagcagg
     6241 ctgctgccgc cggtgaggaa aacgccggcc gcaaggcggt cgaggtgccc ctcgcagcgc
     6301 aggcgcacct gctcgaagat ttccctcacg cgcagttgga tgatttcgtt gagaaaggcg
     6361 cgctcgacct ctccgatggc gtagccgttg tcgtcctcga tgcgcacgat ctcacccgtg
     6421 gccgcatcgt ccacgatgca gctgccggtg ttgattttca ggctctccgc cctggcatga
     6481 tagatgtgca gggcctgggc gatgtcctgg ctgatgtgat cgccccccag cggcacgcag
     6541 ccggcggtgg tgagcatgct gtcgtcgtaa agcacgaagt ccgcggtgcc gccgccgatg
     6601 tcaatcatga gggcgccctc gcgcttggcc tcgcggctga gcacgacctg cgcggcggcc
     6661 acggggttga agacgatgtc ctcgacctcg agaggcatct ccttgacgca cttgatggag
     6721 ttttgaatgc gcggcatctg gccgtggatg atgaggtagt cggcctcgat ctggcggccc
     6781 tggcgcccga cgggcaggcg cacgccctcc acaccgtcca cgatgtactg cctggagacg
     6841 ctgtgaagaa agatgtggtc ctcgggcaca tccacagtct cgcgcgcggt ctttttggcg
     6901 gcctcgacat ccttttcgag aatctcggtg cgtccttcgg gcaggcagtg caggccgcgg
     6961 ttgttgatgc tctggatgtg cgcgccggag actccgagaa agacggtctt gaccatcaca
     7021 tcgctgaggt cctccgccct cacgagcgcg tcattcaggc aggtctgggc tttttcgaag
     7081 tccacgatct cgcctttgcg cactccgcgc gagggtgcct ggccgacgcc caggatcttg
     7141 atggcgccgt cgcgcttggc ctcgccgacg acgacgcaga ttttactcgt gccgatttcc
     7201 agggccacat agatggtgct ttttgccatg ttgattcagc gcgtggtggt tgccgcattg
     7261 gcggcgggca tgcccagccc ggcgaggtcc ggcggactgc ggaaagtgac gggaacattc
     7321 ttgctgacgg ccaggttcag cgtgtccaca tcccagcggc gctgacgggc atggaaccag
     7381 aattggtcca gccgcgccaa ctgcctttcc aattcatcca ccccgaagat gacctgcgtc
     7441 ttgtcggaaa actggaccag aatggaccag ggcgtctgga tgtgcatcgc ccgcgcctga
     7501 ggctggccgt cctcgtggcg ccggtccagt tctttgacga ggtggagggc cgagcgcagg
     7561 gaaagatcgt ccaaagccag gccgggagtg ttttgctgga gcctttcgtg atggatgacg
     7621 ggcagggcgg cgtaattgtc cggctgataa tcgcagggaa acgtggctcc ctcgctgtcc
     7681 accagccagc aaccctctgc tcgcccctcc gccatgccga gacgcgggca ggccagccag
     7741 gcgacgggac ggcgctgcgc gacgcgcagt gtcagcacgc ctttgtcaaa atcacgctcc
     7801 aatgccactc cggtgaccgc cggcaattgg cgcaggcggg cgcgcacctc gcgcatgttg
     7861 atgaacagca aattttcccc gtgcgtcagc agcgtcgtgc gcgcgatctt gtccacggaa
     7921 agcggcccgc tcgtgtccac cgcgatctgc accagcgcaa actgcgggtt tttcagaatc
     7981 gtctcctgca ccgtcacctt cagaaggccc aaaacgcaga tggtgatgat gaggccggtg
     8041 gccagtttga agccaagcct tgtcgcggac tgccgcgcca tctggcggat tttgggcgaa
     8101 tccactggca actcggccgc ataaatgatc ctgggtttcc tggcggactt gcggacgttt
     8161 ttttcatcca ccaaggcagc cccgcgcttc gacgcgggat tctgccggac cggagcctgg
     8221 ggacgggagg aaggcatgac cgtgaaagtg ttgattttta atttatctga aataatctct
     8281 ttcccgctca aatgccataa ttttctcaaa aaatgagcgc cagcattttt tcacaaatcc
     8341 gtctgaaagc gccaaacgcc gcatcttcag acagcagatc ccccgccgcg ctgcggtgca
     8401 aatcaaggcc gcgcctgcaa ggatagctca atgatccgcg cgcacagctc gctgaagctg
     8461 atcccggcgg cgagtgccgc cttgggcagc agactgctgc tcgtcatgcc ggggatggtg
     8521 ttgatctcga gcacaaacgg ctgcccgtcc gcgcgcagca tcacatccac ccgcccatag
     8581 acctccgtgc cgcaggagcg gaaagcggcc atggcggcat cctgcacgcg acgcgtcacg
     8641 gcatcgtcca ggtccgccgg gcaatggtag agcgttctgc ccttgccggt catccagggg
     8701 tatttgttgt tcatgtcata aaagccgtcc accggctcga tgtgaatgac cggcagcacc
     8761 aagtctccca gaatgccgac agtcagttcc ctgccctcaa tgaactcctc caccagcgtg
     8821 gcgtcgccat gccggcgcgc ctcctccaca gcggcggccc attccgatgc gtcgcggaca
     8881 atcttcacac ccacactcga accttcgcgc ggcggcttgg agaccagcgg cggctgaatc
     8941 tccagaggga cgccgccatc cagcggccag atctgggagc gcggtgtcgg caccccggcg
     9001 gcgagaaatt tctccttgct cagcgccttg tcaaaagcca cccggctgct ctccacaccc
     9061 gcgccagtgt agcgaatgcc gcgccgttcc aggatggcct gaatctgccc gtcctcgccg
     9121 aaggtgccgt gaatcaggtt catggcgatg agcacatccg ccgggtattc gaagtccggc
     9181 ccgcgcacat ccaccggcag cacctcagcg ccgagagact ccagcgcctg ccccacactc
     9241 tcggcggttt tcaccgagac ttcgcgttcc gagccggggc cgccgaaaag cacggcgatg
     9301 cgttgtgatg agaggtcgag catgggctgc gcatccatgt cacgccccgc gcgccgcgca
     9361 aaggcaaagt ggcgcgtccg cccagtggaa ggaaaaaagc ccgggcaaca cccgcctcaa
     9421 tgcctttcct gctcgcggaa ctggctcggc gtgagcccgg tcatgcgctt gaagtccttg
     9481 ctgaaaaaaa actggtccgt gtagcccgcc tggcgcgcga tttccttcac cgcgtcatcg
     9541 gactccgcca gacggcgttt cgcctggttg atgcgctcgc gccgcagcca gtcaatcgga
     9601 ctcgtgccca caaacgcctt gaactgccgg ctgaagtggg actcgctcat gcccgccagc
     9661 gcggcgagtt ccgccacgcg gatcggttcg tgaaaataca gccgcatccg ctccagcgcg
     9721 cgctgaactg gctgcggcag ttccggccta agctcgagcg cgtcaccgag ccgcgactca
     9781 aaggccgccg ccagcagcgc cgccacccga gcgttcacat gcgccgcatc acatggacgg
     9841 cctgattcca gccggtgaaa gatgccttca aaatccccct tcgccgcttc cggctccaca
     9901 cccgcgaaaa ccggcctgga ccgcacctca agcagttccc ccagccggtc cagcgtcctc
     9961 ccttcgacac gaacccaata gagatcccag ggatcctgct tcacggcgcc atacagatgc
    10021 ggatggtggc agttcaccca caccagatca cccggccccg cctcatggcg gcggcccgcc
    10081 acctgcaccc agccccgtcc gcccaggcag aggatcaact catgccccgg atacgtctcg
    10141 cgcccgatgc ggtggcccgc gccgccgcgc agatgccctg cgcgcatcac gcagaaaaac
    10201 agatccctct gccactccag gggagtcgca tgatagttgc gcaaaaacac cgtctccttg
    10261 gcgggctggc tcatgggctg caaacgaatc gcatattatg ccggatgtcg agccgcaaac
    10321 ccggcctttc ccccacccgc cgcgccatcc ccggtcttga ccggtcgcgt ctgccgcctt
    10381 tgatgccatc gcgcgtccac cgcgcgcgaa acccgaaccc aaatcccatc atgtccctct
    10441 ccaccggctc acccgcccct gatttcaccc tcgcaaccct cggcgccaac ggccctgaat
    10501 tgttcaagct ctccgagaac ctcggccagg caaacctgct tttgctcttc gtgcccatgg
    10561 ccttcaccgg cgtctgcacc aaggaattct gcgagatctc caacggcatc aatgaattca
    10621 ccaagctgaa cgccagggtc gtaggcatca gcggtgacaa cccttttgcc cagaaggctt
    10681 gggccgaaaa ggagaacatc acgctccagc tcctcagcga ctacgaccac catgtcgcca
    10741 ccgcctatgg cgtggcgtat cagagcttcc tgcccgcgaa aaacctcatc atgggcggcg
    10801 tgcccaagcg ctccgccttc atcgtggaca aggccggcat catccaatac gccgaagtcc
    10861 tggaaagtcc cggcgacctg ccaaacttcg acgccatcaa gtcaaagctc gccgaacttg
    10921 cctgatcccg cccgccgcgg ccacgcggca gcctcagcca cccagccgga ggccggcccg
    10981 acaaggccgc ctccggtttt ttgcgctccg caaaatgcgg ccgggaaaag ccattgccca
    11041 cacgcgccga agcgcgtttc ctcccctgcc atgatggact tcccgcccga caccctgcgc
    11101 gatctgcgcg tgagttatga tctcgacaac ctcgtcgagg aaagcgcgcc cgccgatccg
    11161 cgcgtccttt tccaggcctg gctgaaagac gcgcttgagc accagctccc cgaacccaac
    11221 gccatgaccc tcgccaccat cggcgaagac ggggggccga gcgcgcgcac cgtgctcctg
    11281 aagggcctcg attccaaagg cttccatttt ttcaccaatt atgaaagccg caaagcgcgg
    11341 gagatcgccg ccaacccgcg cgccagcgcc gtctttctct ggacgcagcg ccaccatcaa
    11401 gtctgcgtgc gcggcttcat caaaaagctg ccgcgcgagg agaccgagag ctacttcgcc
    11461 acccgcccgg cggggcatca aatcggcgcc tgggtttccg agcagagcag ggtcatcccc
    11521 ggccgcgcct ggctggaggc gcgcgaagcc gaaatgcgcg cccgcttcgg cgaaggcccc
    11581 atcccctgcc ctcctcactg gggcggttac gcgctagtgc cggatgagtt cgagttctgg
    11641 cagggccgcg tcagccgcct gcacgaccgc ctgcgctaca ctcgcgaagg cgcgggctgg
    11701 aaaatcgagc gtctcagtcc ttgaggcaac atcccgcggc gcggcgcgtt ttttccagcc
    11761 ccatgaaacg ccgccgcttt ctctccacca ccgccgcctc caccttgtcc gcgccgtttg
    11821 tccgcgcgca aagcaagacc tccctgaatg gcgccaccat cggccatggc gcgcaccgct
    11881 accaggtgga cctcgaatgg tgcaaggcgg accgggccgc acaccccgtg aagaactgcc
    11941 acgagatggt catggactcg caaaaacggc tgatcatgat caccgacgag gccaggaaca
    12001 acatcctcgt cttcgacaag gaggggaaag tgctcgacgc ctggacactc aacctgcgct
    12061 ccggccacgg cctcagcttg gatgcaacgg gtggcaaaga gcatctcatt ctctgcgacc
    12121 cctccagcgg caccgtggtg aaaaccacac tcgacggcaa agaggtcatg cgcctgcctc
    12181 acgccaggga ctgcggcgcc tacgatgccg cgatgaaata cgcgcccacg gaggccgtca
    12241 ccgctcccaa cggcgacttc tatgtggccg acggctacgg ctcccagttc atcctccact
    12301 tcgacgcgcg ggggaagttc atccgtaaat tcggcggcca gagcacgcag gccatgaacc
    12361 cgggaaaatt catgcaggct cacggcgtgg ccatggacct gcgcggcccg gagccgcttg
    12421 tcctctgcac ggagcgcgtc cgcaacgagt tccactggta ctcgctcgac ggcatctacc
    12481 tgcgcaccgt ctatctgccg ggcgccttca tgagccgtcc agtcatcaac ggcgacctcc
    12541 tcctctccgg tgtctgcttc ggcatgaaac ccggcgacta ccgcatgtgg cgcgagcgcg
    12601 gtttcatcat catcctcgac agggaaaacc gcgtcgtctc agtccccggc ggccacgagc
    12661 cggttcacga tgacaaaggc caggtggcgc tgctgctgca aaacgaaccc gtgttccgca
    12721 acgtccatga cgtctgcgtt gatgacgccg gcgaccttta cgcctgccag tggaacgccg
    12781 accaggccta tcccttcaaa ctgcggcgcg tgtgatgacc gggaagacgg aaattgccct
    12841 gccgctcact ttttctcccc cgcaaaaatc acccgcaccc ccggcagccc cgccgccaaa
    12901 gccgtcccgc gctgctcgcc gagcacgcac atggcggtag ccagcgcgtc actcgtggcg
    12961 cagtcagggg cgatcaccga gcacgcgacg cgttccgtca gccccatgcc ggtgaacggc
    13021 gagacaatgt gcgagtaacg cacgcctccg atctccgtga actgataaag atcacccgaa
    13081 gtggaaacgg cgcactcagc cagcaccagc gcgcgcagtt cgtcgtcccc gccgggcttt
    13141 gaaaaggtgc gcagcttgat ttcccagccc tcctctccgg gcggcgggct gccaaccgcc
    13201 gtgtcaccgc ccgcctgcac caccgcgcgc gtgatgccgt gccgtttgag cagcctcaag
    13261 cattcgtccg ccgcccagcc tttggcgatg ccgcccaggt ccagcaaagt cccctccgca
    13321 agagtgaccc ctcgtgtctc cggctcgaag ctcatccgct gccagtccgc agccttgagt
    13381 ccgcgctgca gacggtcctc cggcggcagc tttccagtgc ggcgggtccg cctccagagt
    13441 tgcgtcaggt gtccgcacgc cggattgaac gcaccctgag tcagcaccgc cagctcgcgc
    13501 gctttgagca aaatgtcata aagctccgcg ctggtctgaa acggatgcac cgcgcctgga
    13561 gcgcacagac gcatcaactc gctcgcggga tcatagtcgg tgcaaatggc atggagaaaa
    13621 gccacgcgct cgaagcattc ccgcgccgct ctttccgcca catcgcgcgt ctccgcatgg
    13681 caggcgatgc gaaacgtcgt cgccattccc ggcccgctga agtcaaacct ctccagcgcc
    13741 ctgccggaca gcggcaggca cagcaacaag ggcagaagtc ggttggaaac gggcacgccg
    13801 cagttaaacc gctttccgcg cgaaggcaaa actcacacgg ctccaagttt ccggtaaagc
    13861 ctctccaact tgtccggctg gcggcaggag acggccgctt cggcaaggat catcgcgaca
    13921 tcgagccggg agaccttcac cccgcgcaag acctcatccc atgaggcggc gcgcatcttt
    13981 gcaagggtgc gcgcggcgag cagcagcggc agcgcggtgg catagcgcag cttcccgtcc
    14041 gccacatggc gcagatacgg caggccggat tcgaggtgct tttcacagat cccggcccag
    14101 tgatcccatg ccgcgcgcaa ggcggcaacg tcggcggagc cgtccttcaa aacacccggc
    14161 agatagcagc gtccgtcgcg cagatcctcg ccgacatcac gcaggatgtt gacgagttgg
    14221 aggcccttgc caaacgacgc cccccatttc ctcatctcat caagtgacat gtccgcctcc
    14281 agcgcgccgg ggctttccgt ggcgcacatg tccgtccaaa actcgcccac gcaacccgcc
    14341 acgagccagg tgtattcctc cagcgcggcg gcatccggca gggagcgcaa gcgtccgtcg
    14401 gccgggaacc gctcgatgtc gagcatctgg ccgtggatga tgtggtggag cacgccgcgg
    14461 atggcacgca aaggccccgg cctcatgatg ccaagccagg cgagcgcccc ggcaaaacgc
    14521 tccatgagcc gtcgctcggc ggcatcgctc tgcaagggcg cgaattcgcg cgcgatgcgc
    14581 gcggaaagct ctcctgactc ggaggcggag gcggctccct gcgcgcatga ctcataaagc
    14641 cgcaggcact ccagccgcgt cgccgccggc acggtcacgg tgtccgccag agtgtccgca
    14701 gtgcgcgcca gcaaataggc cagggagagc ggctcgcgca gcccggcggg cagggctttg
    14761 agcgttagat aaaaggagcg ggagacggag gccagcaact ggccgcccag ctcccgctcg
    14821 tggcgtgact cgtcagacat ggatgacgat cttgccgaaa tgcgcgccgc tctcaagtct
    14881 gcggaaagcc tcatgcgcct ggtcaaactc gaaggtggag tcaatgaccg ggcgcaggcc
    14941 ggccgccgtc atgacgccga gcatttccac aagcatggag cgggagccga catacacccc
    15001 gtcgaggcgt atgcccttgc gcaggatgcc aacggtgcgc acatcacccg ccgtgccggt
    15061 gagcacgcca atgagcgcga tgtgcccgcc gaagcgcgcg gcgttgatcg agcggttcag
    15121 cgtctgcgga ccgccgatct cgaccacctt gtccaccccc cttttttccg tctgcctctg
    15181 cgcccacgca tcccaggccg gatcggtttt gtagttgtgg actttttcag cccccagttc
    15241 gagcagccgg gccgcctttt catcactgct ggtgatggcc agcacgcgcg cgccagcctg
    15301 gcgggcaaac tggagcgcga aaatggatac cccgcccgtg ccctggatga gcaccgtctc
    15361 gcccgcgcgc acacggccgc ggacatgcag cgcgtcccat gctgtgaccg ccgcgcaggg
    15421 cagcgtggaa ccctcgtcaa aggagaagtg gcccggcagc cgcaccaggc tttctgcgcg
    15481 caggcacacc agctcgcgca gcatcccgtc gcacgctccg cccagcgcct gcgcggcgtg
    15541 catttcagtg aagtccccgt ccagccatgc aggcatgaac ggaaccacca cacggtctcg
    15601 aggtttccag ccggtgacgc cgaaccccgt ggaaaccacc tctcccgcgc catccgagca
    15661 cgggatgcgc ggcacggggc cggtgatgcc gtgcaaaccc gtgacattca tgtggtcacg
    15721 gtagttcaat gacaccgaat tgatccgcac cagcacctcg cccggcttgg gaaaaggatc
    15781 gggcagctcc acccgccgca gggaggccag accttcagtg ccggtgattc gatatgcctt
    15841 catgagtttg cggtggtggc gatgcctgat tgcacgcggc gaatcaaaaa tggcagccgt
    15901 ccaatgcaac gtgccacaaa ggcttccgtt tgatactgtc tggatgcgga cacatggtta
    15961 tgtgacctcg atacgcgcgc gcgccgccct catcggagcg tcatcatcca cgccacgagg
    16021 tccgcgaggc cttgcgggcc gatggcaccg actaagccgg ggggcatgag tgagtctttg
    16081 agttcttctt tttttaccac gttgaccttt ttgacggtgg tggaggcccc accggccatt
    16141 ctcagcgtga gttcggcatc cgtttcactc acgacatagc ccaccactgt ctgcccgtcc
    16201 ttcgtcgtca ccgtcagtcc gacataactg tgctcgatgg cggcgctggg gtagaggatg
    16261 gcggtgaaga ggccgtcctg gctgagcttc gcgccgattt gcgtgagatc ggggccgaag
    16321 tcgatgaact ggcctttgac gcggtggcag gccacgcaac cggcctgcat gaacatggcc
    16381 tgacctttgg tggcatcgcc cttcagcttc aggagttcgg gcagtttggg gaaattcgcc
    16441 aaaccttgcg ctgcgggcag cgggagctcg cgggaagctt gatcgcgcag accggcatcg
    16501 gtgctgcgac tgagcgcgag ggcggcctga tctttgaatt gaggcggcaa tttgccttcc
    16561 gactgccatt tcaggatggc gcgaccacct tcgccgctga gcgcgagggc gtcgatgagc
    16621 acgcggcgca cgtcctcgcg cttctccggc cagaaatgct ggttcatgac cgccaccgcc
    16681 gcgcgatcac tcacgcgacc gagcgccgcg atgagcgcct tggccgattt cgcgtccgca
    16741 tccttcaaaa agcgcaacgc gccatcccgg ttcgccgcga tgagccgcgc ggcgttcact
    16801 gactccggcg cgttcgggtt cgcgctgatg aaatcgagca gttccttctc aaagcccgtc
    16861 aggttcagtt tttcgaccag cgccacaaac tccggtttgc ccaccaccgg cttcaaaagc
    16921 gagtcgagcc gcgctttggc ttcgggattc gaggcgatgg cattgcggtc gagcagcgcg
    16981 gtggaagcga gggcggtttc ggcatcggct ttgaggaaga tcgtgagggc ggctttggcg
    17041 acttcttcgc gatgatggta ggagaggagt tgcagagcgc ggagaaagtg gaggctatta
    17101 ttcttcgccc aagtcgagtg ctcgtctatg ctgttgataa acatcgaggc tatcaccatt
    17161 ggcgtgagac tgcttctatg acggcgaatt aattcagctc tagtcgctga ttcagtattg
    17221 ccttttgcct tgccatggaa tggataaaag cgtgagtccc cctgaaggtc ctcgccgata
    17281 ccgagagctt cgatcatcca gcggtctgaa gtcgcgcatt gctccccaat actacaccat
    17341 aggccactac cgaggtcagt accgtcaaat cgcaaggcaa tggctacttc tcgacgaacg
    17401 gctggtgatg tatcgctggc cgctatgccg agtgcttcac gcagtttttc cctttccgct
    17461 cggtacaaga caccatcttt ctgccacaac ctcctcgcca gtcgaacagc agtgagacga
    17521 atgttcatat ccgagtcttt gagcgcatta ctcaatgaga aggtgttttt ctcttcggct
    17581 tttctcgaaa cgattgcagg cttcttgaat tccccttcgc cgatagccca cagcgctcgt
    17641 gctcgatgct gcggctcggc tttcttgtcg tcgaacaatt gcttcagcgt gggttcggct
    17701 ttggttccaa gtttcttcaa cgcctcccac gcgagatacc gcgcagcttc attcggagac
    17761 ttcaaggcat cgatggcacc ttcgacagtc tccaacttga aacttggaac ctgaaacttc
    17821 tccgccgcct tcgtcgtcac gcgaaacaac ctcccgcgtg tcacatcgcc catgccgtgg
    17881 ccgccgacgc cggggtcata ccagtcggcg acgatcaaac tgccatcggg ggcgacggcg
    17941 acgtcgctgg ggcggaacca gcggttgtgg gtgccgtcga ggaggttgac aatttcggct
    18001 ttgtagccgg cgccgtcggg ctgggtgatg tagccgcgga cgacgttcgg gccggcgtcg
    18061 cagtggaggg gctggccgtg gaagatgctg ggcaataggt cgccttcata gacctgcatg
    18121 ccggtggggc tgcctgcgcc ggtcatgagc atgttcggga cgacgccggg atcgttttga
    18181 tgccagtggc gctgcggaat ttcactctcc cagccgatgc gcggcgtctg ccagcctgcg
    18241 ccggtcattt cgtcggcgta gccgtagttg ccgaactcca tgacgtagtt gatgcgcacg
    18301 cctttgttgc cgtcgtcgtc gttgtcgctc tgccacatcg tgccgaacga atccacggcg
    18361 acttcccagt tgttgcggaa gttccaggcg agcacctcga ctccgctgcc atcgagttcg
    18421 cagcggaaga ccatgccttg ctgatacggg cggttgtttt gattggtgca cttcacgccg
    18481 tggatgtcgg tgatgaggtt gccgtttttg tcgcagagct gcttgccggc gttgccgaaa
    18541 ttgaagtaga ggcggccatc agggccgaag tggaaggcgt ggatgccgtg gtcgtgctgc
    18601 gcaccgccga tcttcgtgaa caggaactct ttgcggtcgg ctttgtcgtc gccgttgtcg
    18661 tcgatgagcc agaaaacgtc gtcaccgcag gagatgaggg ctttgttgcc gagcacgcag
    18721 atgccgtgcg cgctgttcac gtcggggcct tgatggaaga cggtggattt atccgccttc
    18781 gcgtcgccat cggtgtcttc gaggatgagg atgcggtcgc cctcggcgcg ttcgccgttg
    18841 tgcttgcggt agttcacgac ctcgcacacc cacacgcgtc cgcgatgatc aatgtcgatg
    18901 ctggcgggtg aaagcatcat cggctcgctg gcgaagagct gcacgaggca ctcgggatga
    18961 acgtcgagct gctcggtggc ggcttccggc tcgtgcgagg cggcgttgtt gtcactggcg
    19021 gcgatggggc cgggattttc ggtgaagaca ctgaaggtga cgctggtgct gtttccctgc
    19081 ttcgtgccgc cttcatcgag agcgccgcgc gctttgaagc gtttcacgcc tgcgggtaga
    19141 ttgaaggcga tgacactgtt cgcgtgcgtg ccgatgccgt attcgatggc tttcccggcg
    19201 ctgcgcagct cctggccctg tgcattcgca tttacccgca cgccacccca gccggactgc
    19261 gcggatttcc actcgatttc ggtgagtttc ttctcactgc catcgccaag cacgagacgc
    19321 ggctccgccc agtcggccca atccgcggtg aaaccgttcc cagcgtcggt gacgaccaaa
    19381 aacagctctt tcgcaccttc gagcgggacg tcgatgtcca cgctgtggtt cggcgttttc
    19441 gtgtcgatga ccggcgagcg gaaaagcggc ttcggcgcgg ccaaagcgag cgtgggcagg
    19501 agaagtgtga gggcgaggcg catggcgatg tgattgaaaa gtgagaattg gaaactgaaa
    19561 aatgaggagg acgaccggca gctagaccag tggacattga atctcgccca cttgcgcgct
    19621 caaggaggca gccgccacgc catgcagcgg aagggatgat cgtctatgag gttggcacgc
    19681 acaccggtga ggcggctggc atcgtaaaat tgcacgccca cataataata aaccgggatg
    19741 agcgcggcgc ggtcggtgat cagatgctgc tccgcctgct ggaagacccg gttgcgcgcg
    19801 gcggggtctg gctccgcggc agcggcgcgg atgaggcggt cgtattccgg gctggcgaag
    19861 cctgtgcggt tttgcccgtt gccgctctgg aagaggtcga gaaaggtgct ggggtcgttg
    19921 taatcaccca cccaactgct gcggcacagg tgatagtcca gctcgcgcat ggatttgagc
    19981 cagacctttt gctcctgctt ggccaggttc accgtgacgc caagcgcgtc ctgccacatg
    20041 gcctggagtt cgacggcgat gttccgctcc accgcgagcg ggatgtagag gtactcgaca
    20101 cgcggaaaac ctttgccgcc aggatagccg gcctcggcca gcaactgacg ggcgcgcgca
    20161 gggtcgtggt caagaccggg cggcggctgg tagtcacgcc cggttcccgg cggcacgatg
    20221 ccgcgcgcgg tggattcgcc gagccgggtg attttttcca cgatgcgttt tttgtccacg
    20281 gcgagcgcga acgcctggcg cacgagcgga tcgtcgaaag gcgggcgggt gttgttgatg
    20341 cggatgaacc aggtgccgag gaaagggccg gtgtggaacc agggctgctg cttgagcttt
    20401 tgcgtgagtg tgggaggcac catgcccttg tccatgagga ggtcgcaccc gcccgtgtgg
    20461 aagcaggcca gcgcggtgcc ggcatcctgg gatgggcgca cctccaccgt ggtcatgcgc
    20521 acgttttccg cgtcccagta ggcggggttt ttgcgcaggc ggatgcggtc gtcgagccgc
    20581 cagtcttcaa gcaggtaggg gccgttggtg acgatgcagc ccggtttgat ccaggcggtg
    20641 cctttttcct cgatcacccg cagcggcacg ggcgcgaagg tggcgaatga gcagaggtct
    20701 atgaaatagg gcacggggtt ctccaactcg acgacgaggg tgcgctcatc aggcgcgtgg
    20761 aggccgactt gatcccaggc cgccccctgc tcggtgaagg cgcgcgctcc gcggatgatg
    20821 tgcagcaggc tggcatagtc cgcgccggtg tcgggagtca gggcgcgccg ccaggagcgg
    20881 aggaaatcct gcgcggtgac aggcgtgccg tcgctccaag ctgcgccggg gcgaaggtga
    20941 aaggtgtaag tcttgcggtc ctcagagatt tcccaccgca gagccatgcc gggttcggga
    21001 cggccatgtt cgttgacgcg gcacagtcct tcgaaaagcg ccgccgacac gcgcatcgag
    21061 acttggtcgg tggcgagcgc cgggtccagc gtctccggct cggcgctctg aatgaaaacg
    21121 aggtcggcgc gcggcagagt ctccccgcag gcagcgagca gcagggcgca ggcggtggcg
    21181 gcggagaggc ggtgcatcat gcggcgcgca gtctggagcg aggcggcggc cggcgcaaga
    21241 ggggcggcat cgctgaaaat aacaaccgcg atgcccggca ggggcatcgc ggctgcgggg
    21301 atgaggggcg cgcgctgtca gggagcgggc gtggtctccg cgggcgccgc ggatttttcg
    21361 gctgcaaact tgaggcgctt tgcctcctcg tcgaatgaga ccctcaccct gtcgccctcg
    21421 cgcacgtcgc cgcggaggag atgctcggcc agcggatcct cgatgtgctt ctccacagcg
    21481 cggcgcatgg ggcgcgcgcc gtattgcgga tcgtagccct ccttcatcag gaactcgacg
    21541 gcgctctcct ccatttcgag aatgatattc ttgcccttga tgcggctgac gaccttggcg
    21601 atttcgaggt ccacgatgac gcgcagctcg gtcttttcga gcatgcggaa gacgatgagg
    21661 tcgtccaggc ggttgaggaa ctcgggcttg aagaacttct tcgccgcctc gccgagcttg
    21721 cccttgatgc cggcattgtc cgcctccccc tcggccatgg cggcgaagcc gaggctggtc
    21781 tggcgcttga tctgctcggc gccgacattg gaggtgagga tgacgatggt gttccggaaa
    21841 tcaatcttgc gcccgaagtt gtcggtgatc tggccctcct ccagaatctg gagcagcagg
    21901 tgcatcacat ccgggtgggc tttttcgatc tcgtcaaaaa gcacgaccga gtaggggcgg
    21961 cggcgcacgg cctcggaaag ctggccgccc tcctcgtagc caacgtatcc cggaggcgcg
    22021 ccaatgaggc gggaggcggt gtgtttctcc atgtattcgc tcatgtcgag ctggatgagc
    22081 gcctcggcgc tgccgaacat gaactcggcc aggtttttcg ccaggaaggt cttgccaacg
    22141 ccggtggggc cgaggaaaag gaacgagccg atggggcggc gcggatcctt gaggtcggcg
    22201 cgggagcggc gaagggcttt ggaaatggcc gccaccgcct cgtcctggcc gatgaccttg
    22261 cctttgagtt cctgctccat cttgagcagc ttcgcggcct cggcctgctc catgcgctgg
    22321 agcggcacgc cggtccactt ggagacgacg gacatgatgt catcctcgga cacgtccacg
    22381 atctgctcct tgttgttctt ccgccacgtg tcgaagagct cgtcgtaacg gttcttggcc
    22441 ttgcgctcct tgtcccgcag gcgggcggct ttctcgaagt cctggtcctt gatggaggtc
    22501 tccttttcga cgcggatgga ctcgatctcc tcctcgatgt ccttgagctg cggcgggcgc
    22561 gtcatggccg cgatgcgcgc cttggccccg gcctcgtcca tgatatcaat ggccttgtcc
    22621 ggaaggaagc gcgccgtcag gtaacgggcg ctgaggttga ccgccgtgcg gatggcctcg
    22681 tcgctgtagt tggccttgtg gtgcatctca tatttgctct tcaggcccat gaggatcttg
    22741 atggcgtcct caatggaggg ctcctccacc ttcacctgct ggaagcggcg ctccagcgcg
    22801 gagtccttct caatgtactt gcggtactcg ttgagcgtgg tggcgccgat gacctgcagc
    22861 tccccgcggc tgagagcggg cttgatgatg ttgctggcgt ccatggtgcc ctcggcggag
    22921 ccggcgccaa cgatggtgtg catctcgtca atgaaaagga tcacattctt gttgcgccgg
    22981 atctcgtcca tcaccgcctt gatgcgctcc tcgaactggc cgcgatactt cgtgcccgcc
    23041 accatgagag cgaggtccag cgtgatgaca cgcttgtcgc gcaaaatctc cggcacgttg
    23101 ccaacggcga tctcctgggc cagcccctcc acgatggccg tcttgcccac acccgcctcg
    23161 ccgatgagaa cggggttgtt cttggtgcgg cggcagagga tttgaatgac acgcgcgatc
    23221 tcgtccgcgc gcccgatgac ggggtccatc tcccctttct gcgccacctc ggtcaagtcg
    23281 cgcccgaagg cgcgcagggc gggcgtcttc tcgtttttct tcttcttttt ttcctcgtca
    23341 acgctgccgc ctggggtcac gttttccgcg tcgtcgtcat cctcgcccgc cgcgccgccg
    23401 gagaagttgg gatccagctc gcgcaggatc tcgttgcgcg ccttgtccag gttcacatcc
    23461 agcgtgcgca gcacctgcgc tgccacgccc tcgccctcgc gcagcaggcc gagcagaatg
    23521 tgctccgtgc cgacatagga gtggttgagc gccttggcct ccttgctggc cagtgccagc
    23581 accttcttca cacgcggggt gtaggggatg ttccccacca tctttgtctc cgggccggag
    23641 cccacctgct gctccacatg caggcgcacg gtctccaggt cgaggccgag cttggtgagc
    23701 acattcacgg ccacgccctg ccccagcttg atcaggccca gcagcaggtg ctcggtgccg
    23761 acgtagttgt gattgaatcg gtcggcctcc ttgcgggcga gggccagcac ctgttgagcg
    23821 cggggggtga aattgttcat ggtgtgtgtg ttttgaaacc ttctccaggc tttctgctca
    23881 aagcataaaa gcggcctgcc gtgaatgcaa cgcaagcgtc ataaaacctt ggcgcctgcc
    23941 gtgctgtttt ggatgccgcg tcacactgac gcctccgccg cgccgaggcg ggggcggcta
    24001 gggggcgcgc agctccgcgc c
//
LOCUS       DAOMYJ010000059        23945 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Prosthecobacter sp. isolate AMR_MDS_5629
            NODE_761_length_23945_cov_21.198786, whole genome shotgun sequence.
ACCESSION   DAOMYJ010000059 DAOMYJ010000000
VERSION     DAOMYJ010000059.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867850
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prosthecobacter sp. (wastewater metagenome)
  ORGANISM  Prosthecobacter sp.
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Verrucomicrobiaceae; Prosthecobacter.
REFERENCE   1  (bases 1 to 23945)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 23945)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.30
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:34:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,742
            CDSs (total)                      :: 4,684
            Genes (coding)                    :: 4,676
            CDSs (with protein)               :: 4,676
            Genes (RNA)                       :: 58
            tRNAs                             :: 55
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23945
                     /organism="Prosthecobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_761_length_23945_cov_21.198786"
                     /isolate="AMR_MDS_5629"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1965333"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(175..879)
                     /locus_tag="PLP58_08290"
     CDS             complement(175..879)
                     /locus_tag="PLP58_08290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ08551.1"
                     /translation="MIRRFPPAHPASHQPQEQDGLLHFDSAPEFGSGTHDDDLPAEDM
                     EKQVRETQERLARLRDEQEAVEREQQMLESLKQKKERFGTGRKEVMTLLEQHLRTIGH
                     DLEQARRHVEDLAATENDFREHLEHLKNFLPERWQRGNIMQEMDRALAVLEEAEAALE
                     TGILRIAAHQPRQAPPPARSWFRSRRDEEMEEDAAAGHSAAHYDLTLWAWRGLAFTLP
                     LIIAMLLGLVLAKFMF"
     gene            complement(913..2652)
                     /locus_tag="PLP58_08295"
     CDS             complement(913..2652)
                     /locus_tag="PLP58_08295"
                     /inference="COORDINATES: protein motif:HMM:NF028259.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ08552.1"
                     /translation="MQAFAATLLLARAHLRMMRQRTRHALRTNRLLLLTIGGFLGLYC
                     AAAYVLVARGLDFIAKMPLFGPLLTERMVHLLFFFFFVMLVISNATITGMGLFRRKEM
                     DWQVALPVPWTSLVMWKTLEGMALASWGLLVLSAPILAALGRVYHAGPFFYLASVPAL
                     LCLVSLSASISTWLLLILVRHARRWWWRPAAVLGAALLVLALKNWFSQGGPEDLAGGD
                     LVATMQEVMKHTEICTHPLLPSSWVAEVVFAAGREAEGRAGFFILVLLANALFALLVT
                     ARLGAVMFYPAWHRVMAAVPLARATDKADPWYRLPAKKNSGFWRRLWGLDRASYAVVL
                     KDVRTFLREPAQWGQSLLIFGLLLMYTSNLRKLGYDLQSPFWVIVISHLNLLVCCLAL
                     STLTTRFVFPQISMEGQRLWILGLSPVSLARVLGLKLRLSAGVMALLTMMLVIVSSLS
                     LALPWERALFFCIAVVFMSYGLNALSLSLGALLPNFREPNPARIISGFGGTLCLITSF
                     LYILIAACILAAPEVWRWKSRVIGKPPPMELTWSQDALALILVFIVTLFFGALPYAFA
                     KKRTKNLDYLAES"
     gene            complement(2752..3330)
                     /gene="pgsA"
                     /locus_tag="PLP58_08300"
     CDS             complement(2752..3330)
                     /gene="pgsA"
                     /locus_tag="PLP58_08300"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR00560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008444 -
                     CDP-diacylglycerol-glycerol-3-phosphate
                     3-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HRJ08553.1"
                     /translation="MNLPNQLTVSRLLLTGVFVACFYLPWENSYSIAVIVFGAASFTD
                     YLDGAIARARNLVTNFGKLFDPLADKILIAAAFILLSVEKDIPSWITILILAREFFVT
                     GIRQIAASQGAVLAAEKLGKHKMLWQIITVLYFMLAAASEEPLFAFARPAFEPSPAHE
                     WIGGFIIYFTTALTVVSGLSYFWKNRRLFADA"
     gene            complement(3404..3970)
                     /locus_tag="PLP58_08305"
     CDS             complement(3404..3970)
                     /locus_tag="PLP58_08305"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HRJ08554.1"
                     /translation="MTETPDDEDRDLMLALAGGNDPALNVLMRRWSPRLVSFLERLTG
                     SHATACDLAQETFVRVYKHRFRYRPALKFSTWLYTIAGNLARNQARWQKRHPLTLMEP
                     GELHRIEPEAETPTPAEDMEKQERARAVRQAVMKLAPEQRETLVLSTYESMSHAEIAG
                     IMGTTTKVVEMRLYRARKELREMLRKLL"
     gene            complement(3967..4464)
                     /locus_tag="PLP58_08310"
     CDS             complement(3967..4464)
                     /locus_tag="PLP58_08310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ08555.1"
                     /translation="MTLKLKALLLALGMVVIFIAAHDLVLEIGPRQPTPQEAGLAWLK
                     SEYRIPDESFEKIKALHEEYFARCDAMCAQMLAARGTAPRVPTRNVPAENVRLMRQRA
                     EAAGRAREKALCESCLETMVSHLETVAALMTEGQGERFLKDLLPDLVNPRELQELRAQ
                     TRPVQ"
     gene            complement(4471..4779)
                     /locus_tag="PLP58_08315"
     CDS             complement(4471..4779)
                     /locus_tag="PLP58_08315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ08556.1"
                     /translation="MNTPENDPLDDLLLEWQVRGETPASQQREVWARIAAGEAEPAWW
                     ESFLLLMLRPRSLALTAAAAVVLGVLSALLEMRAPEISPHDAYVQCVSPFASVHLASH
                     "
     gene            complement(4819..5070)
                     /locus_tag="PLP58_08320"
     CDS             complement(4819..5070)
                     /locus_tag="PLP58_08320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ08557.1"
                     /translation="MMKPTLLILAAAMLTTGVSCSSMGTDSTGRRYYHSSAPIQSKTG
                     LTFKETRTRAISAAPSQVRYHHSPAPIPSKTGLQVAHVR"
     gene            complement(5250..6176)
                     /locus_tag="PLP58_08325"
     CDS             complement(5250..6176)
                     /locus_tag="PLP58_08325"
                     /inference="COORDINATES: protein motif:HMM:NF015867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspK"
                     /protein_id="HRJ08558.1"
                     /translation="MNKLKLELQRRFQSGAALLAVLWVIALLIGLVAGVSLLLTQDVD
                     LAATKRQVFRARMLSEAALAIAMNPDIKPDDMLLRRQVAEDEGWVVEMTGEDGLINPN
                     ILLQREDRPTLIRIFRHWGMSLAQANTLIDRMLDWVDVDDFARVQGAESKAYNQAGFP
                     FNRPFRSIEEMALVQGMEIVEAAYPQWRTWFSIHASGILDVNETAPEILAAVTGADIR
                     LAQNLRSQRIGRDGLLNTQDDLPMPDLTSALAVLGLPGQPDAWAQILGTQSATKRILV
                     KVQVGDLERQVAAVVRGAIGQGATAILWMGER"
     gene            complement(6299..7135)
                     /locus_tag="PLP58_08330"
     CDS             complement(6299..7135)
                     /locus_tag="PLP58_08330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HRJ08559.1"
                     /translation="MRTGIRNSKFEASSRGPLTAGEIPNFGFRVSSFHIRAFTLLEMM
                     VALSAFVLLMGGIFAIANSTMELGNDLAISQDRSRVRLNFIEFLRRSFRNLPGEAEIR
                     LSVQPKGGTYLPTLNIVNGGSCFTPGGALPPDTSVDLFAEERPGGYLRVGIRVLDDRQ
                     TAAVRAGQQVRYARNQESIALLDNVSRFEWRYFDGQSNRWENNWKDARRPLLAEMSLR
                     LDDGFETRAVFWIPPVIRQAGVGAVPIAPGGVPTGPGGAPIGPDGQPLNPGGQPLPPG
                     NP"
     gene            complement(7140..7646)
                     /locus_tag="PLP58_08335"
     CDS             complement(7140..7646)
                     /locus_tag="PLP58_08335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HRJ08560.1"
                     /translation="MKFEIRNSSIERPGRRAVAKEGPLPASNFEFQASNFHRQRAMTL
                     LEVVLALAVFSIAALALVGTINQIAEAGIESQRLLEIEQGLESLIDEFGKAPQLKELD
                     QQIKPGADGVAYRVVIQLVRDLKNQDGRFLQNTYRIVVTARWSEGGDPIEVSAETLRY
                     AGSFLPVN"
     gene            complement(7649..8134)
                     /locus_tag="PLP58_08340"
     CDS             complement(7649..8134)
                     /locus_tag="PLP58_08340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ08561.1"
                     /translation="MSLRPCQLNQCLLRVASQGSTLIELITVMSIMLIITGVGVLSFT
                     YFDDEDDPFAKTAQKLVQMSKYAHQSAVLQHRRMIIGFDEEGFGVLGESAGDHARQAL
                     PQDMKVFILRYGAKNWEKAEGHLWPFGEQGICEPVKIRLETRTDFRELAFHPLTGALV
                     E"
     gene            complement(8213..8632)
                     /locus_tag="PLP58_08345"
     CDS             complement(8213..8632)
                     /locus_tag="PLP58_08345"
                     /inference="COORDINATES: protein motif:HMM:NF019934.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspG"
                     /protein_id="HRJ08562.1"
                     /translation="MKNFTTPLVKSTQYAFTLMEMILVIAIIAVLIAVGAGTLGDFGV
                     QAEITAARGQIKTLESAVRMYKVNNRTLPPSLEALVKPPANALVKRPFVENESAITDP
                     WGTKYQFRSPGKDGRSFDIYSMGPDQREGTDDDVFSH"
     gene            complement(8715..9971)
                     /locus_tag="PLP58_08350"
     CDS             complement(8715..9971)
                     /locus_tag="PLP58_08350"
                     /inference="COORDINATES: protein motif:HMM:NF012694.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HRJ08563.1"
                     /translation="MPNFAYQAADITGRDISGTVEAADRAAAIRQLTGRGLQPFKVAE
                     TASAGLAAKAAKKQARADEAAEGETGPLKLSNSQVQLFTEELAELLEAGMRLEAALRL
                     MEGKNAKTTQSRVARKLGNLIREGHPFSSALRQTSPSFNELFCAVAAAGEAGGSLGTA
                     LKRQARYLGNVAEMRSQVAVALIYPGFLFVSAIGVIFLFTTFLIPRLSGMMSRMKGGI
                     PKGLQIVMAISDFMKNYWWLLLAASALIALGFWVWTQSKAGRPVWDQGKLRIPFIGTV
                     LMTSFHTQFLETLASLSSGGLPLLKGLELASRVSSNLYVQKQLENVIASVRDGASLSR
                     GLEKTALFPLRLLEMVRIGEHTGDLAGTLRRAADRCGRELSKALEKVMAMLQPVIILL
                     MAALVGVMAYTMISVIYDTVSAINNR"
     gene            complement(10063..10773)
                     /locus_tag="PLP58_08355"
     CDS             complement(10063..10773)
                     /locus_tag="PLP58_08355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ08564.1"
                     /translation="MSLTALKCPSCSSAIREPDIDLSRGLAKCSYCGSVMNLTASFGG
                     SAQGGGKREPVALPKRMTFEKTLHGIEITRSWFTPVVWALVFFCVIWDGFLVVWYAMA
                     FTAGAPMIAKLFPVIHVTVGLVLSYYAVASFVNKTHVTLERGILRIAHRPLPWPGARE
                     LAGMSLRQLFCKEHISRGKNGVTVTYELWALHDDGSTKTKLAAGMELEQALYLEQELE
                     KALGIQDRPQPGEVPGRA"
     gene            complement(10834..11745)
                     /locus_tag="PLP58_08360"
     CDS             complement(10834..11745)
                     /locus_tag="PLP58_08360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009959256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HRJ08565.1"
                     /translation="MMKPLDLQVNGYAGTDFNRDGLTAEALHHACTCLLEDGCDSILA
                     TFITDDLDAMSRRMARLVELRAQDSLAQEVIAGIHIEGPFINPEKGYVGAHPPQHVKP
                     AGIEDAKRLLDAAGGLARIVTLAPECDERFRVTEFLANYGVTVSAGHCNPQLDVLRAA
                     TEHGLKMFTHVGNGCPMLMHRHDNIIHRALSLRDRLWLCFIPDGVHIEFFALLNYLRV
                     AGLEKTIFVTDAISASRLGPGKYTLAGWDIVIGEDLVARSPDGSHFVGSTVTIPRILS
                     NGQQHLGLSKDELKLLLDTNPRKAIGL"
     gene            complement(11951..12910)
                     /locus_tag="PLP58_08365"
     CDS             complement(11951..12910)
                     /locus_tag="PLP58_08365"
                     /inference="COORDINATES: protein motif:HMM:NF013704.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006306 - DNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA methyltransferase"
                     /protein_id="HRJ08566.1"
                     /translation="MSSFRLILGDCFEELLKLPANSLDAICTDPPYGVVEYSETEVAK
                     LRAGRGGVWRLPPKFDGKERRPLPRFSILTLEQRLQVEEFFAVFSRLILQKIKPGGHM
                     VIAGNPVLQHHVQAGIMKAGWENRATLLRVYHGFRGGDRPKNAEAEFPEVCVTPRGNY
                     EPWMLFRKPLSEKTVAANLRRWGTGGLRMLAGSRPLPDVIPSFKTPSRERAIADHPSL
                     KPQHLLRILVRSLLPTGSGIVLDPFMGSGSTLAACEAVGCDSIGVERDPAYFSLAQTA
                     IPRLARLYPSFDGNTLETNEFCEAPRQVDEQLVLLEEPERFCV"
     gene            12967..13854
                     /locus_tag="PLP58_08370"
     CDS             12967..13854
                     /locus_tag="PLP58_08370"
                     /inference="COORDINATES: protein motif:HMM:NF024949.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HRJ08567.1"
                     /translation="MDAKKTLGKAIRQLREALMLSQEKLAENAGITYQYLSAVENGRE
                     NFTIGILESLAGALGTSTASLVAHAFNPNPEPPPKVARPFFIKDASLPPGLTFQDLEK
                     ALNETQGVIRMINATLIQVTARPLSAYIQRNNFSGIVSNILCDSFSRFTPFKHNHDQR
                     YPDLIHKTPDGEKVGLEVKSTIRPGKGGESHNGHSGWHVVACFSLDETNGEIEFVHVM
                     FADLIGHGKARADWKYVGSNVNADTGSQRTETYTTTPAGTAKLRHGTVYLNSKKISIA
                     RWRTDKSIPAPPSSPFRGR"
     gene            14008..15135
                     /gene="lpxB"
                     /locus_tag="PLP58_08375"
     CDS             14008..15135
                     /gene="lpxB"
                     /locus_tag="PLP58_08375"
                     /EC_number="2.4.1.182"
                     /inference="COORDINATES: protein motif:HMM:TIGR00215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008915 - lipid-A-disaccharide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-A-disaccharide synthase"
                     /protein_id="HRJ08568.1"
                     /translation="MPAHLHILAGEVSGDTHAAGLVRELRAADPELRFTGLGGPRLRE
                     AAGQGIEDWVETAGVVGLWEVLKMYGYFKKRLAAEVARVLRERPAAVILVDYPGFNLR
                     LAAALRKGGYTGRLIYYISPQVWAWKKGRVKVMAKVLDLMICIFPFEKDFYEQSGLRT
                     EFCGHPMVDRMVALRRNWKREPGLIGWFPGSRFNEVRRLFPVMLAAACAIRLAVPEAR
                     FAVSAANERIAGHLREMADAAAMPEAKAWIETGTVYDLMQRAQAGAVASGTATLEAAC
                     FGLPYALVYQVNALTYAVAKTVVKIEHIGIINVLAKREVVRELVQGELSSDSLAAEMV
                     SLLTDTDRRARLEGELAAVVVTLGEGGAYARAAAVVMETLR"
     gene            complement(15136..17826)
                     /locus_tag="PLP58_08380"
     CDS             complement(15136..17826)
                     /locus_tag="PLP58_08380"
                     /inference="COORDINATES: protein motif:HMM:NF016488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RyR domain-containing protein"
                     /protein_id="HRJ08569.1"
                     /translation="MSAGKHIPLDDTSRRDPGLWPRLSDPDWVALHAPRFTELRPRFE
                     SCAAALTAVLNMARKKLAVEAIIQVRAKEVPSFAEKILRKRDGYSKPKPGLPPDPLAR
                     LTDLCGGRVICQTSDQVRAVTAWIGEHFEIDWENSDDASGRLRATEFGYRTVNRIVSF
                     KPGVFPAEMVPEALCQPVPLPGSDLRLPLKMEIQIRTLLEHAWADVSHDRAYKTEVKL
                     PAHLVRDLHSKAAILEETDRDIARLLAALEDYHSNYGAHHKRAEVAEEIALQRIVLQQ
                     MTRADQRATLAVRIAKLCLAAGDQAGAVEVLAEFAAHDNHDVQRALGQALVEMHQEQP
                     SSAEFGRGLEHLRQASRSRGADAETHCLLGEALLLRDEEDPAAEAYHAAIRLDATEPS
                     SLARYIEHETARQGNVRLILLAEPMIRAAMKRCHTQIQGSVNQPAAWAALALLHLMVN
                     EPNRAVEALAHLAILCEAPPAGDAARQPCAAARHFLRLRRALSRLVCVRDNLDGHAIL
                     ERAVLLMLAGRAKDAEALRELEKLASRHLPDVFRQTAASGGSVLIVAGGCAPEVEPKV
                     ADFEAPFHNAFTGLPLPGRALVLVSGGTRSGISRLAGDLAEKSGGRIKAAGYTPSTPD
                     GCAPDRDTKRYAWLCESAGTDFTALDPLQGWTDLITARVDLKNIKLLAFAPGKIARAE
                     MAVAFALGARVGLVEHPDLPPDRHFDDSCWLEHLGSGSLARMPLDAMTLRAFLLADQI
                     PEADARYEKAARKVHEAYLKSAKPADPSLSPWEDLPPELKVSNYHQVAYWTNILGTEG
                     LGVRPVPAGSEGATFDISAAIGKEGVLRLAEMEHGRWNIERLLLGWRWAEKKDVARKL
                     SPSLIPWDALSPEIQGYDISAIRGLPASLLAAGFEVFKLE"
     gene            complement(17823..18245)
                     /locus_tag="PLP58_08385"
     CDS             complement(17823..18245)
                     /locus_tag="PLP58_08385"
                     /inference="COORDINATES: protein motif:HMM:NF012514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HRJ08570.1"
                     /translation="MIESKPYSLSVKAVILDDSGRCLLLRRAGGSTHFPGCWEWPGGK
                     LDPGESFSAGLEREVLEECGLNLELTGLAGAMEFDMPRARVVALCMEARAGGGALRLS
                     DEHEDAAWVSLSRLKKHKLPGPQRAFMLDYAARKSTTS"
     gene            18409..19920
                     /locus_tag="PLP58_08390"
     CDS             18409..19920
                     /locus_tag="PLP58_08390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009960738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ08571.1"
                     /translation="MTHRRSFLTSATGAGALTALGQLGFLGRLPRVSAAEAKLPPHLV
                     RFHPEIEPLVRLLEDTPRERVLEEVASRVRRGLSYREVLAALMLAGIRSVQPRPVGFK
                     FHAVLVVNSAHLASLASPDSERWLPIFWAIDQFKSSQAANVKEGNWLMKPVDESRVPS
                     SDRAKKAFIEAMDNWDESAADAAITGLVRTAGANELFEIFVRYGMRDFRDIGHKAIYV
                     ANSFRTLEVIGWHHAEPVLRSLAYALLDRAGAKENPAKADLPADRPFRQNLEIVKQVR
                     EGWLDGKRDAGATKELLQAVRTGSPADTSAAALKLLNAGIAPQSVFDALFDGAGELLM
                     QAPGILSLHAMTFTNAIHYAWHHTQSDETRRLLLLQNAAFLPLYRGDRADNGVHIDAL
                     EPITPEAKGEEAVAEIFADIGKDRLTAARKVLGYLRDQNSAQPVADAARRLVFLKGTN
                     SHDYKFSSAVLEDYEHLAQPWRDRLLAASVFYFRGSGEKDNDLVGRIRAALGA"
     gene            19975..21264
                     /locus_tag="PLP58_08395"
     CDS             19975..21264
                     /locus_tag="PLP58_08395"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HRJ08572.1"
                     /translation="MNTTSHSTDRRTFSPDQRMVLWVLWLTYGTFYFTRNNLSVALPG
                     IQEELGYTKSQMGTVLMVLKLAYGAGQFINGQLAEHWQPRKLLALGLLASAALNVVFG
                     WMTALYFMTFVWACNGYVQALGWPPTMRVAANWFPPLQRGRAIGIIGTGYQLCGALTF
                     VVAGWAAERFGWRGALWVPALLVSLSAVHMLVFLKEKPAAEHALEDIEHAAAMANRSL
                     RANLLVTLGNPGLWLVAVALCLLDACRYGFTDWGVTHIKEVQNASVSSAAFKYAVLPF
                     GGIAGAFMSGWATDRFFKGRRAPVICVLMLALGLLALAYDTVIHWGMAPSVAILFGIG
                     FCIFGAQVLLVGTLPVDLARHGTAAAAAGFVNFMGYMGAAAGDKLTGSFAEVHGWPAA
                     VRFWAACAFAGGLVIAFLWKAAPYHQADLKPEPHNQK"
     gene            21264..22019
                     /locus_tag="PLP58_08400"
     CDS             21264..22019
                     /locus_tag="PLP58_08400"
                     /inference="COORDINATES: protein motif:HMM:NF024214.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphocholine cytidylyltransferase family
                     protein"
                     /protein_id="HRJ08573.1"
                     /translation="MKAIIIAAGRGRRLMPTTADSPKCYAEIHGKRMLDHALDAFAGA
                     GVTDVCFIGGYQIDKVRADYPRFTFRHNQDWENNNILLSLMHAEDLMDEGFICSYSDI
                     LFTSDVVKGVLHHPADIALSVDTQWLDRYQHRSQHPPDDAEKVIVSNGRVTRVHRGIL
                     PDEAYGEYTGIAKFTPAGAAKLREHFHQAREQFAGKPFREAVVFEKAYLILLLQQMIE
                     AGIPMAHSDTPGHYMEVDTQEDFELARRHWLRA"
     gene            22367..22591
                     /locus_tag="PLP58_08405"
     CDS             22367..22591
                     /locus_tag="PLP58_08405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ08574.1"
                     /translation="MDAVPAITSAVVDQVETLAVREASVVTASGVNGVLHLSFGIPQG
                     SRMEPKARKARCPRKTSPTASTPAPSHHCG"
     gene            complement(23089..23646)
                     /locus_tag="PLP58_08410"
     CDS             complement(23089..23646)
                     /locus_tag="PLP58_08410"
                     /inference="COORDINATES: protein motif:HMM:NF025432.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4256 domain-containing protein"
                     /protein_id="HRJ08575.1"
                     /translation="MKKELPKKERSAFLNVLQERFAQNPHRHPGIEWEAVRQRLEARP
                     DRLWSLAEMERTGGEPDVTGHDEATGEILFCDCSPESPKGRRSLCYDRAALDARKEHK
                     PAGSALDLAAGMGVEVLSEQQYLALQQLGPFDTKTSSWVLTPPGMRKLGGALFGDTRF
                     GRVFFYHNGAESYYSSRGFRACLRV"
BASE COUNT         4344 a         7209 c         7874 g         4518 t
ORIGIN      
        1 tgagggcgag cggcgcctcg gcgatctcgc attcaagacg gtggatttcc tcccgcaact
       61 gccgtgagcg gctgccaaga tccttgatgc gggatttgga agcgggatcg gcggcgtctt
      121 tggcggattt ggagcgggag gggacgcgtt cgtttttcat gggagcggag tggattagaa
      181 catgaatttt gccaggacga gaccgagcag catggcgatg atcagcggca gggtgaatgc
      241 cagaccgcgc catgcccaaa gggtcaggtc gtaatgagcg gctgaatgac cggcggcggc
      301 gtcttcttcc atctcctcgt cgcgcctgga gcggaaccat gagcgcgcgg ggggtggcgc
      361 ctggcgcggc tgatgggcgg cgatgcggag aatgcccgtc tcgagcgccg cctccgcctc
      421 ctcgagcacg gcgagggcgc ggtccatctc ctgcatgatg ttgccgcgct gccagcgctc
      481 aggcaggaaa tttttcaggt gctccaaatg ctcgcggaag tcattttccg tggcggccag
      541 atcctccaca tgacgccgcg cctgctcgag atcgtgcccg atggtccgca ggtgctgctc
      601 cagcagcgtc atcacctcct tgcgtccggt tccgaagcgc tctttttttt gcttgaggct
      661 ctcaagcatc tgctgctcgc gctccacggc ttcctgctcg tcgcgcaggc gggccaggcg
      721 ctcctgggtt tcgcgcacct gtttttccat gtcctcggcg ggaaggtcgt catcgtgggt
      781 gccactgccg aattctggag cggaatcgaa atgcaggaga ccatcttgct cttgcggttg
      841 atgggaggcg ggatgagcgg gaggaaaacg acgaatcata aatatcgcat taaatatttg
      901 gcgatattag tactatgact ctgctaagta atccagattt tttgtgcgct ttttcgcaaa
      961 ggcataaggc aaagcgccga agaaaagtgt gacgatgaac accaaaatca atgcgagcgc
     1021 atcctggctc cacgtcagtt ccatcggcgg cggcttgccg atcacgcgag atttccagcg
     1081 ccagacttcc ggcgcggcca ggatgcaagc ggcgatcaaa atgtaaagga aactggtgat
     1141 gaggcagagc gtgccgccga agccggaaat gatgcgcgca gggttcggct cgcggaagtt
     1201 cggcagcaaa gcgcccagag agagcgagag cgcgttcagc ccgtagctca tgaagaccac
     1261 tgcgatgcag aagaaaagcg cgcgctccca gggcagcgcg agcgagaggc tggagacgat
     1321 gaccagcatc atcgtcagca gggccatgac cccggcggag agccgaagtt tgaggccgag
     1381 cacgcgcgcg agcgacacag gcgagaggcc gagaatccac aagcgctgcc cctccatgct
     1441 gatctgcggg aaaacaaaac gcgtggtcag agtggacagc gccaggcagc agaccagcag
     1501 gttcaggtgg ctgatgacga tgacccagaa ggggctttgc aaatcatagc ccagcttgcg
     1561 gaggttcgag gtgtacatca gcagcaggcc gaaaatgagc aggctctgtc cccactgcgc
     1621 gggctcgcgc aggaaagtgc gcacgtcttt gagcaccacc gcgtatgagg cgcggtccag
     1681 cccccagagc cgccgccaga agccgctatt ttttttcgcg ggcagccggt accacgggtc
     1741 ggctttgtcg gtggcgcgcg ccagcggcac agcggccatg acgcggtgcc aggcggggta
     1801 aaacatcacc gcgccgaggc gcgccgtgac cagcagggcg aagagcgcgt tggccagaag
     1861 caccaggatg aaaaagccgg cccggccttc cgcctcccgc cccgccgcga agaccacctc
     1921 cgccacccag gagctgggca gcagcgggtg cgtgcagatt tccgtgtgct tcatgacctc
     1981 ctgcatggtg gcgacgaggt cgcctccggc caggtcctca ggcccgccct ggctgaacca
     2041 gtttttcaag gccagcacca gcagtgccgc gcccagcacc gccgccggcc gccaccacca
     2101 gcggcgcgca tggcggacca ggatgaggag cagccaggtg gagatgctgg ccgagaggct
     2161 cacgaggcag agcagcgccg gcacggacgc gaggtaaaaa aatggacccg catgataaac
     2221 gcgccccagc gccgcgagga tcggcgcgct gagcaccagc agtccccagc ttgccagggc
     2281 catgccctcc agggtcttcc acatcaccag gcttgtccac ggaacgggca gggccacctg
     2341 ccaatccatc tccttgcggc ggaagagacc catgcccgtg attgtggcgt tggagatgac
     2401 cagcatgaca aaaaagaaaa agaacagcag atgcaccatg cgctccgtga gcagcgggcc
     2461 gaagagcggc atcttggcga tgaaatccag accgcgcgcg accagcacat aggctgcggc
     2521 gcaatacagg ccgagaaagc cgccgatggt cagcagcagc agccggtttg tccgcagagc
     2581 gtggcgcgtg cgctgccgca tcatccgcag atgcgcgcgc gcgagcagca gggtggcggc
     2641 aaaggcttgc atgaatggca aatgacaacc ggcctggagc gaaagtcaat cagcgcggcc
     2701 attcgcggcg tccggcgggc atgccaagga ggcgcgcctg gtttcccgcg ctcacgcgtc
     2761 ggcaaagagg cggcggtttt tccagaaata actcaggccg gagacgacgg tgagcgcggt
     2821 ggtgaagtag atgatgaagc cgccgatcca ctcatgagcg ggcgagggtt cgaaggccgg
     2881 gcgggcgaag gcgaacagcg gctcctccga agcggcggcg agcatgaaat aaagcacggt
     2941 gatgatctgc cagagcatct tgtgcttgcc cagcttctct gcggcgagca cggcgccctg
     3001 gcttgcggcg atctggcgga tgccggtgac gaagaactcg cgcgccagga tgagaatggt
     3061 gatccaggac gggatgtcct tttccaccga gagcaggatg aaggcggcgg cgatgaggat
     3121 cttgtccgcc agcgggtcga agagcttgcc gaaattcgtc accaggttgc gcgcgcgggc
     3181 gatggcgccg tcgaggtagt cggtgaagga ggccgcgccg aagacgatca ccgcgatgga
     3241 gtacgagttc tcccagggca ggtaaaaaca ggccacgaac acacccgtga gcaagaggcg
     3301 ggagacggtg agctggttcg gcaggttcat gggaggatgc cgcctttgat aacgaaggac
     3361 accgccggat gtcaacgggc ggctgctttt tgtggcgaaa agctcacagc agcttgcgca
     3421 gcatctccct tagttccttg cgcgcgcggt acaggcgcat ctccaccact ttcgtggtcg
     3481 tgcccatgat cccggcgatt tcggcatgag acatgctctc ataggtggaa agcacgagtg
     3541 tctccctctg ctccggcgca agcttcatca cggcctggcg caccgcgcgg gcgcgctcct
     3601 gcttttccat gtcctcggcg ggtgtggggg tttccgcttc gggttcgatg cggtggagtt
     3661 ccccaggttc catcagagtg agcggatgcc gcttttgcca gcgggcctgg ttgcgcgcca
     3721 gatttcctgc gatggtgtag agccaggtgg aaaatttcag cgcagggcgg tagcggaacc
     3781 ggtgtttgta cacgcgcaca aaagtctcct gcgcgaggtc gcaggcggtg gcatggctgc
     3841 cggtgaggcg ttccaggaat gagacgagcc ggggcgacca cctgcgcatg agcacgttga
     3901 gcgcggggtc gtttccacca gccagggcga gcatgaggtc tctgtcttcg tcgtccggcg
     3961 tctcggtcat tggacgggcc gggtttgcgc gcgcagctcc tgcagctcgc gcgggttgac
     4021 cagatcgggc aggaggtcct tcaaaaaacg ctccccctgc ccctctgtca tcaatgcggc
     4081 cacggtctcg agatggctga ccatggtttc gaggcaggac tcgcacaggg ctttttccct
     4141 ggcccggccc gcggcttccg cgcgctggcg catgagccgc acattctccg ccgggacatt
     4201 gcgcgtgggg acgcggggtg ctgttccgcg cgcggcgagc atctgcgcgc acatggcgtc
     4261 acaacgggcg aagtattcct catgcagagc tttgattttt tcaaacgact cgtcaggaat
     4321 ccggtattcg ctcttcaacc aggccagacc cgcctcttgc ggtgtgggct ggcgcggccc
     4381 gatctccagg acaaggtcat gcgcggcgat gaagatcacc accatgccga gcgcgaggag
     4441 cagggctttg agcttgagag tcattccgct tcagtgtgag gcgagatgca cggaggcaaa
     4501 cgggctgacg cactgcacat aggcgtcgtg cggactgatc tccggggcgc gcatctccag
     4561 cagtgcggag aggactccca gaaccacggc ggcggcggcg gtgagcgcca ggctgcgcgg
     4621 gcggagcatg agcaggagga aggattccca ccaggccggc tccgcctcgc ccgctgctat
     4681 gcgcgcccac acctccctct gctgggaggc tggcgtctcc ccgcgcactt gccactccag
     4741 caggagatcg tcgagcggat cattttccgg cgtgttcatg gcggcggggg aggcgtgtct
     4801 ggtttgaacg agggattctc agcggacatg cgccacctgc aagccggtct tggagggaat
     4861 gggcgctggc gagtggtggt aacgcacctg gctgggtgcg gctgagatgg ctctcgtccg
     4921 ggtttccttg aaggttaggc cggtcttgct ctggatcgga gccgacgagt ggtagtagcg
     4981 gcggccggtg gagtctgtgc ccatggagga gcaactgaca ccggtggtca gcatggcggc
     5041 ggcaagaatg agcagggtcg gtttcatcat ggttgggttt ggttggtttg ggtttggttg
     5101 gtttttgacg cgcgcaaagg cccgtcagtg ggtgctaccc cgccgctttt tccacccctc
     5161 aaaaaatgcg atttttttcc gccgtggcag gcgcaagcgt gccgcaggcg cgttccgcgg
     5221 cttgggatgg gcgcgcggga tatgccgcat caacgctcgc ccatccaaag gatggcggtg
     5281 gcgccctggc cgatggcgcc gcgcacgacg gcggcgacct ggcgttcgag atcacccacc
     5341 tggactttga cgaggatgcg tttcgtggcg ctctgggtgc cgaggatctg cgcccatgcg
     5401 tcaggctggc cgggcaggcc gaggacggcg agcgcgctgg tgagatctgg catgggcagg
     5461 tcgtcctggg tgttgagaag gccgtcccgc ccgatgcgct gggagcgcag attttgcgcg
     5521 aggcggatgt ccgccccggt gacggcggcc aggatttcag gcgcggtctc attgacatcc
     5581 aggatgccgc tggcgtggat gctgaaccat gtgcgccact gaggataggc ggcctcgacg
     5641 atttccatgc cctgcaccag ggccatctcc tcaatcgagc ggaagggccg gttgaaggga
     5701 aaaccggcct ggttgtacgc cttgctctcc gcgccctgca cgcgcgcgaa gtcgtccacg
     5761 tccacccagt cgagcatgcg gtcaatgagg gtgttggcct gggccaggct catgccccag
     5821 tggcggaaaa tgcggatgag cgtaggccgg tcttcacgct ggagcaggat gttcgggttg
     5881 atgagtccgt cctcgcctgt catctccaca acccatccct cgtcctcggc cacttggcgg
     5941 cgcaggagca tgtcgtccgg tttgatgtcc gggttcatgg cgatggcaag cgcggcctcg
     6001 gaaagcatgc gcgcacggaa gacctgccgc ttggttgcgg ccaggtccac atcctgcgtg
     6061 agcagcagcg aaaccccggc caccagcccg ataagcagcg caatgaccca caacaccgcg
     6121 agcaaggccg cgccgctttg gaagcggcgt tggagttcaa gctttagctt gttcactgac
     6181 cgcccgcagc ggagtccact tccgtgtcgc ctaaaggctt cactccagcg ttgtagtcca
     6241 agcctgccca tgcctccaga aggcttgact ccaatttcaa gaatgccgaa acgcgttctc
     6301 atgggtttcc tggtggcagg ggctggcccc ctggattcag cggctgtcca tccggaccga
     6361 tgggcgcgcc tcctggtccg gtgggcactc cgcccggggc gatgggcacg gctcccacgc
     6421 cggcctgcct gatgacgggc ggtatccaaa agacggcgcg tgtttcaaag ccatcgtcaa
     6481 ggcgcaggct catctccgcc agcagggggc ggcgcgcgtc cttccagttg ttctcccagc
     6541 ggttgctctg cccgtcgaag tagcgccact caaagcggct gacattatcc aggagggcga
     6601 ttgactcctg gttgcgcgcg taccgcacct gctggccggc gcgcacggcg gcggtctggc
     6661 ggtcgtcgag cacgcggatg cccacgcgaa gatagccccc gggcctctcc tcggcaaaaa
     6721 ggtccacgct ggtgtccggc ggcagcgcgc cgccgggtgt gaagcaggag ccgccgttga
     6781 cgatgttcag cgtgggcagg taggtgccgc ctttgggctg cacgctgagg cggatttcgg
     6841 cctcgccagg caggttgcgg aaggagcggc gcagaaattc gatgaagttc agccgcaccc
     6901 gcgagcggtc ctgggatatg gcgaggtcgt tgcccagctc catggtgctg ttggcgatgg
     6961 cgaagatgcc gcccatgagc agcacgaagg cggagagcgc caccatcatt tccaggaggg
     7021 tgaaggctcg gatgtgaaaa ctcgaaactc gaaatccgaa attcggaatt tcccctgcgg
     7081 tgagaggccc gcgtgatgag gcttcgaatt tcgaatttcg aattccggtt ctcatgactt
     7141 taattcaccg gcaaaaaaga cccggcgtag cgcagggtct ccgcgctgac ctcgatgggg
     7201 tctcctccct cgctccagcg ggcggtgacg acgatgcggt aggtgttctg caaaaagcgc
     7261 ccgtcctggt ttttgaggtc gcgcacgagc tggatgacga cacggtaggc cacgccgtcc
     7321 gcacccggct tgatctgctg gtccagctcc ttgagctggg gtgccttgcc gaactcatca
     7381 atcaaggact ccagcccctg ctcgatctcc agcagccgct gggattcgat gcccgcctcg
     7441 gcgatttggt tgatggtgcc caccagcgcc agggcggcga tgctgaacac ggccagcgcc
     7501 agcacgacct ccagcagggt catggcgcgc tggcggtgga aattcgaagc ttgaaattcg
     7561 aaattcgaag caggcaacgg accctccttt gccacagcgc ggcggcccgg cctttcgatg
     7621 ctcgaatttc ggatttcgaa tttcatgctt actcgacgag cgctccggta agggggtgga
     7681 aggccagctc gcggaaatcg gtccgggttt cgaggcggat tttcaccggc tcgcagatgc
     7741 cttgctcgcc aaacggccag aggtgcccct cggccttctc ccaatttttg gcaccatagc
     7801 gcaggatgaa gactttcatg tcctgcggca gcgcctggcg ggcgtggtcg cccgcgcttt
     7861 caccgagaac gccgaagccc tcctcatcga agccgatgat catgcgccgg tgctggagca
     7921 ctgcgctctg gtgcgcgtat ttggacatct gcaccagctt ttgggcggtt ttggcaaagg
     7981 ggtcgtcttc gtcgtcaaag taggtgaagc tcaagacgcc cacacctgtg ataatgagca
     8041 tgatactcat cacggtgatc aactcgatca gcgttgagcc ttgcgacgcc acgcggagaa
     8101 gacattggtt caactggcaa ggacgaaggc tcatgatttt tgtgtttgtt gagatttggg
     8161 ccttcatcca cgccgctttt ggaactgcca agaaccaaat tatgagaatt ggtcaatgag
     8221 agaaaacatc atcgtcggtg ccttcgcgct gatctggtcc catcgaatat atgtcaaatg
     8281 agcgcccgtc ttttccaggg ctgcggaatt ggtacttggt gccccaggga tctgtgatgg
     8341 cggattcatt ttccacaaat ggcctcttca caagcgcgtt agccggaggt ttaaccagcg
     8401 cctcaagact tggagggagg gtgcgattgt tgaccttgta catccgcaca gcagattcga
     8461 gcgttttaat ttgaccgcgt gcagctgtga tttcggcttg cacaccaaag tcacccaggg
     8521 ttcctgctcc tacagctatc agcacggcaa tgatcgcgat gacaagaatc atctccatca
     8581 acgtgaatgc gtattgtgtg gatttgacga gtggtgtggt gaagtttttc atggtcgtgt
     8641 gttacggatt gagtttggag tttgagatag ccggattcgt tgaagaacga tgacattaat
     8701 cttcgggatt gccatcagcg gttgttgata gcggaaacag tgtcgtaaat aactgagatc
     8761 atcgtgtaag ccatgacacc gacgagggcg gccatgagca ggatgatgac gggctggagc
     8821 atggccatga ctttttccag ggccttggaa agctcgcgcc cgcagcggtc ggcggcgcgg
     8881 cgcagggtgc cggcgaggtc gccggtatgc tcgccgatgc gcaccatttc cagcagtctc
     8941 aggggaaaga gcgcggtttt ctccagtccg cgcgagaggg acgcgccatc acgcacgctg
     9001 gcgatgacgt tctcgagctg cttctgaaca taaaggttcg atgagacgcg ggaggccagc
     9061 tccagcccct tgagcaaagg caggccgccg ctggagaggc tggccagcgt ttccaggaac
     9121 tgggtgtgga agctggtcat gagcacagtg ccgatgaagg ggatgcgcag cttgccctgg
     9181 tcccacacag gcctgcctgc cttggactgg gtccagaccc agaagcctag cgcgatcaga
     9241 gcgctggcgg ccagcagcag ccaccagtag tttttcatga aatcggagat ggccatgacg
     9301 atctggagac ccttggggat gccgcccttc atgcgcgaca tcatgccgct caggcgcggg
     9361 atgaggaagg tggtaaaaag gaaaatgacg ccgatggcgg agacgaagag aaaaccggga
     9421 tagatgagcg ccacggccac ctggctacgc atttcggcca cgttgccgag gtaacgggcc
     9481 tggcgtttca gcgcggtgcc cagggagccg cccgcctcgc cggcggcggc caccgcgcag
     9541 aagagttcat taaaagaggg ggaggtctga cgcagagcgc tgctgaaggg gtggccttcg
     9601 cggatgaggt tgcccagctt gcgggccacg cggctttgcg tggttttggc gtttttcccc
     9661 tccatgagcc gcagggcggc ctccagtctc atgcccgcct caagcagctc ggcaagctcc
     9721 tcggtgaaga gctggacctg gctgttgctg agcttgagcg ggccggtctc gccctcggca
     9781 gcctcgtcgg ccctggcctg tttttttgcc gctttcgcag ccaatcccgc gctggcggtc
     9841 tcggccactt tgaagggctg caggccgcgt ccggtcaact gccggatggc ggcggcgcgg
     9901 tcggcggcct cgacggttcc ggagatgtcg cgtccggtga tgtcggcggc ttgataggca
     9961 aagttcggca tgggcttggc agggctgaga caagcacagt ccgggtttgt tgcaatccgg
    10021 catctgccgc gctttgtgac gcggccgcga cacgcgccgg catcaggcgc gtcccggcac
    10081 ctccccgggc tggggccggt cctgaatgcc gagagctttt tccagctcct gttccaggta
    10141 tagcgcctgt tccagctcca tgccggcggc cagtttggtc ttcgtggagc cgtcgtcatg
    10201 caacgcccac agttcgtagg tgactgtgac gccgtttttc ccgcgcgaga tgtgctcctt
    10261 gcaaaaaagc tggcgcaggc tcatgcccgc cagctcgcgc gcgccgggcc agggcagggg
    10321 acgatgggcg atgcggagaa tgccgcgctc cagggtgaca tgggtcttgt tcacgaacga
    10381 ggccaccgcg taatagctga gcaccaggcc cacggtgaca tggatcacgg ggaacagctt
    10441 cgcgatcatg ggcgcgcccg ccgtgaaagc catggcatac cagaccacca ggaagccgtc
    10501 ccagatgacg cagaaaaaaa ccagcgccca caccacaggc gtgaaccagg agcgcgtgat
    10561 ctcgatgcca tgcagggtct tttcgaaagt catgcgtttc ggcagtgcca ccggctcgcg
    10621 cttgcctccg ccttgggccg agccgccgaa ggatgcggtg aggttcatca ccgagccgca
    10681 gtaggagcat ttggccaggc cgcgggagag gtctatgtcc ggctcccgga tggcggagga
    10741 gcaggacggg catttgaggg cggtgaggga catgcgggaa agctactcgc gtctccggcg
    10801 ggccgccagt gcgggaaggg cgcgcgcggc gaatcacagc ccgatggcct tgcgcgggtt
    10861 ggtgtcgagc agcagcttca actcgtcctt gctcaggccg aggtgctgct ggccgttgct
    10921 gaggatgcgc gggatggtga cggtgctgcc gacgaagtgc gagccgtcag ggctgcgcgc
    10981 cacgaggtcc tcgccgatga cgatgtccca cccggccagc gtgtacttgc cggggccgag
    11041 gcgtgaggcg gagatggcgt cggtaacgaa gatcgttttt tccagcccgg ccacgcgcag
    11101 atagttcagc agcgcgaaga actcgatgtg gactccgtcc gggatgaagc acagccagag
    11161 gcggtcgcgc agactgaggg cgcggtggat gatgttgtca tgccggtgca tgagcatggg
    11221 gcagccgttg cccacatggg tgaacatttt cagcccgtgc tccgtggccg cgcgcagcac
    11281 atcgagctgg ggattgcaat gcccggcgga gacggtgacg ccgtagttgg caagaaactc
    11341 cgtcacgcgg aagcgctcgt cgcactccgg cgccagcgtg acgatgcgcg cgaggccgcc
    11401 cgccgcgtcg agcaggcgct tcgcgtcctc aatgccggcc ggcttcacat gctgcggcgg
    11461 gtgcgcgccc acatagcctt tttccggatt gataaaggga ccctcaatgt gaatgccggc
    11521 gatcacctcc tgagccagcg agtcctgcgc gcgcagttcc accagccgcg ccatccggcg
    11581 gctcatggca tcgaggtcat ccgtgatgaa ggtggccagg atcgagtcgc agccgtcctc
    11641 cagcaggcag gtgcaggcgt ggtgcagcgc ctcggcggtg aggccgtcgc ggttgaagtc
    11701 ggtgccggcg tagccgttca cttgcaggtc gaggggtttc atcatgccgc gagtctatca
    11761 ccggcggcgg gcgcgatgcg agttttgaaa tgccgcgctt gagtcttttt gtgacgcccc
    11821 ccggcctgcc aagctttgct gcttttcata tttcagccag cccctggcgc ttttcgacca
    11881 catccaggca gatccagacg gaggaggttt ccgatagtcg agaaacgcgg cagtttagcc
    11941 attttgatgt tcaaacacag aagcgctcag gttcctccaa aagcaccaac tgctcatcta
    12001 cctgccgggg tgcctcacag aactcgtttg tttccaaagt gttgccatcg aaactgggat
    12061 agaggcgcgc caggcggggt atggctgttt gcgcgaggct gaaatatgcg ggatcgcgtt
    12121 caacgccaat agagtcacag ccgacggctt cacaagccgc aagggtggaa ccggaaccca
    12181 tgaagggatc gagtacaatg cctgaaccgg ttggcagcag acttcgcaca agaatacgca
    12241 ggagatgttg cggtttgagt gaggggtggt cggcgatggc gcgttcgcgt gatggcgttt
    12301 tgaaactggg aatcacatcg ggcagcggac ggcttccggc gagcatgcgc aaccctccag
    12361 tgccccatcg gcgcaggttt gcggcaacag ttttctctga aagcggtttt ctgaagagca
    12421 tccagggttc gtagttgccg cgtggagtga cacaaacttc cgggaactcg gcctcggcgt
    12481 ttttcggccg gtcaccgcct ctgaatccat gatacacccg caaaagtgtg gctcggtttt
    12541 cccagcctgc cttcatgatt ccggcttgaa cgtggtgctg caagaccgga ttacctgcga
    12601 taaccatgtg ccctccgggt ttgatttttt gcaaaatgag cctggaaaaa acagcaaaga
    12661 actcctccac ctgcaagcgc tgctccaggg tcagtatgga aaaccgggga agcggcctcc
    12721 gttccttgcc gtcgaacttg ggaggcagcc tccacacacc gccgcgcccc gcgcgcaact
    12781 tggcgacctc ggtctcacta tactccacga ctccgtatgg aggatcggtg cagatggcgt
    12841 ccagagaatt tgcggggagt tttagcaact cctcaaaaca gtctccgaga atcaaacgga
    12901 aggaactcat tttctactat agttgaaaga caatgtttga ggcactcgct agcgtcaaga
    12961 gttttcatgg atgcaaaaaa gactcttgga aaggccattc gccagctcag ggaagcgctc
    13021 atgctttcac aagagaaact ggcggaaaat gcggggatca cataccaata cctgtccgcg
    13081 gtggaaaacg gcagggaaaa cttcaccata ggcatcttgg aatccctggc tggcgcgctg
    13141 ggcacctcaa ccgcgtcttt ggtcgcgcac gctttcaatc cgaaccctga gccgcctcca
    13201 aaagtggcga ggcccttttt catcaaggat gcgtcacttc ctcccggtct gacttttcaa
    13261 gacctggaaa aagcgctcaa cgaaacccag ggagtgattc gcatgatcaa cgcgaccttg
    13321 atccaggtga cggcccgccc tttgtccgcc tacattcagc gcaacaattt cagcggtatt
    13381 gtttccaata ttctctgtga ttctttttcg aggttcactc ctttcaaaca caaccacgac
    13441 cagcgctacc cggacctgat ccacaaaact ccagatggag aaaaggtcgg tttggaagtg
    13501 aaatcaacca tacgtccggg caaaggtgga gaaagccaca acggccattc cggctggcat
    13561 gtggtggcct gtttttctct ggatgaaacc aacggcgaaa ttgaatttgt tcatgtgatg
    13621 ttcgccgatc tcatcgggca tggaaaagcc agagccgatt ggaaatatgt gggcagcaat
    13681 gtgaatgcgg atactggcag ccagcgaacg gagacgtaca ctaccacacc cgccggcacc
    13741 gccaagcttc ggcatgggac cgtatatctc aactccaaga aaatttcgat tgctagatgg
    13801 cgcactgaca agtccatccc ggcaccgccg agttcgccgt ttcgcgggag gtagccgcat
    13861 acggttggcg gcagtttgaa tcagccttca acgattccga attcttatag cagcgtggaa
    13921 tggctccgtc cgcgccatga cttgcataac gcaagccgat ggttgatctc cccctccgcc
    13981 cgtgcgaatg ccgcgccttt cgaagtcatg cccgcccatt tgcacattct cgccggagag
    14041 gtcagcggag acacgcacgc cgccgggctg gtgcgggagc tgcgcgcggc ggacccggag
    14101 ctgcgcttca ccgggctggg cgggccgcgt ttgcgcgagg cggccggaca agggatcgag
    14161 gactgggtgg agacagccgg tgtcgtcggc ctgtgggagg tgctgaagat gtacggctac
    14221 ttcaaaaagc gcctggccgc cgaggtggcg cgcgttttgc gggagcggcc cgccgcagtg
    14281 atcctggtgg actaccccgg cttcaatctg cgcctggctg ccgcgctgcg gaagggcggc
    14341 tacacggggc ggctcatcta ttacatcagc ccgcaggtct gggcctggaa aaaggggcgc
    14401 gtgaaggtca tggcaaaggt tctggacctc atgatctgca tcttcccctt cgaaaaggac
    14461 ttctacgagc aaagcggctt gcgcaccgag ttctgcgggc accccatggt ggaccgcatg
    14521 gtggcgctgc ggcggaattg gaagcgcgag cctggcctca tcggctggtt tcccggcagc
    14581 cgcttcaacg aggtgcgccg gctcttcccc gtcatgctcg ctgccgcgtg cgccatccgc
    14641 ctcgccgtgc cggaggcgcg ctttgccgtc tcggcggcca atgagcgcat cgccgggcac
    14701 ttgcgcgaga tggccgacgc cgccgccatg ccggaggcga aggcctggat cgagaccggc
    14761 acggtttacg atctcatgca gcgcgcccag gccggcgcgg tggccagcgg caccgccacg
    14821 ctggaggccg cctgcttcgg gctgccctac gcgctcgtct atcaggtcaa tgccctcacc
    14881 tatgccgtgg cgaagactgt ggtgaagatc gagcacatcg gcatcatcaa cgtgctcgcc
    14941 aagcgcgagg tggtgcgcga actcgtccag ggcgagctga gcagcgactc actggctgcc
    15001 gagatggttt ccctgctcac cgacacggac cgccgcgccc gcctggaggg cgagctggcc
    15061 gccgtggtcg tcacgctcgg ggagggcggc gcttacgctc gcgccgccgc cgtggtgatg
    15121 gagactctgc gttgatcact ccagcttgaa gacctcgaac ccggcggcga gcaggctcgc
    15181 gggcagcccg cggatggcgg atatgtcata gccctggatt tccgggctga gcgcatccca
    15241 gggaatgaga ctcggggaga gcttcctggc cacatccttc ttttccgccc agcgccagcc
    15301 gagcagcagg cgctcgatat tccagcgccc gtgctccatt tcggcgagcc ggagcacgcc
    15361 ttcctttccg atggcggcgc tgatgtcgaa ggtcgcgccc tcggagccgg cgggcaccgg
    15421 gcgaacgccg agtccctcag tgccgaggat gttcgtccag taggccacct ggtggtagtt
    15481 cgagactttg agctcgggcg gcagatcttc ccacggggaa agcgaggggt cggcgggctt
    15541 ggcagatttc aaataagcct cgtggacctt ccgcgccgcc ttctcgtagc gcgcgtcggc
    15601 ctcggggatt tggtcggcga gcaaaaaggc gcgcagcgtc atcgcgtcca gcggcatgcg
    15661 cgccagcgag cccgagccga gatgttccag ccagcaggag tcgtcaaagt ggcggtcggg
    15721 cggcaggtcg ggatgctcca cgagaccgac gcgcgcgccg agggcgaacg cgaccgccat
    15781 ctcggcgcgg gcgatttttc ccggcgcgaa ggcgagcagc ttgatgtttt tcaagtccac
    15841 tcgcgccgtg atgagatcag tccagccctg gagtggatcg agcgcggtga agtccgtgcc
    15901 cgccgactcg caaagccatg catagcgctt ggtatcgcgg tccggcgcgc agccgtcggg
    15961 ggtggagggc gtgtaacccg ccgccttgat cctgccgccg gacttttccg ccaggtcgcc
    16021 tgccagccgg ctgatgccgg agcgggtgcc gccggagacc agcacgagcg cgcgtccggg
    16081 cagcggcagc ccggtgaagg cgttgtggaa gggcgcttcg aaatccgcca ccttcggctc
    16141 cacctccggc gcgcagccgc cggccacgat gagcacgctc ccgccgctgg cggcggtctg
    16201 gcggaagaca tcaggcaggt gtcttgacgc cagtttttcc agttcccgca gcgcctccgc
    16261 atccttggcg cgccccgcca gcatgagcag aaccgcgcgc tccaggatgg cgtggccgtc
    16321 caggttgtcc cgcacgcaga ccaggcggct caaggcgcgg cgcaggcgga ggaaatgacg
    16381 cgcagcggcg cagggctgcc gggcggcgtc cccggcggga ggcgcctcgc agaggatggc
    16441 caggtgggcc agggcctcga cggcgcggtt cggctcgttg accatgaggt gcagcagcgc
    16501 cagcgcggcc caggccgcgg gctggttgac gctgccctgg atctgcgtgt ggcagcgctt
    16561 catggcggcg cggatcatcg gttcggcgag caaaatgagg cgcacattgc cctgacgcgc
    16621 cgtctcgtgc tcgatgtagc gggccaggct ggagggctcg gtggcgtcga gcctgatggc
    16681 ggcatggtag gcctccgcag ccgggtcctc ctcgtcgcgc agcagcagcg cctcgcccag
    16741 caggcaatgg gtttcggcgt ccgctccgcg cgagcgggag gcctggcgca ggtgctccag
    16801 ccccctgccg aattccgcgg atgaaggctg ctcctgatgc atctccacca gcgcctgacc
    16861 cagcgcgcgc tggacatcat gattgtcatg ggcggcgaac tccgctagca cttcgacagc
    16921 gccggcctgg tcgccggcgg ccaggcagag tttggcgatc ctcaccgcca gcgtggcgcg
    16981 ctgatccgcg cgcgtcatct gctggagcac gatgcgctgg agggcgatct cttcggccac
    17041 ctccgcgcgc ttgtggtgcg cgccgtagtt ggagtggtag tcctccagcg ccgccagcag
    17101 gcgcgcgatg tcgcggtccg tctcctccaa aatggctgcc ttgctgtgca ggtcgcgcac
    17161 gagatgcgcg ggcagtttga cctccgtctt gtaggcgcgg tcgtgggaca cgtccgccca
    17221 ggcgtgctcc agcagggtgc ggatctggat ttccattttc agcggcagcc gcagatccga
    17281 gcccggcaac ggcacgggct ggcacagagc ctccggcacc atttcagcgg gaaagacgcc
    17341 gggtttgaag gagacgatgc ggttgacggt gcggtagccg aactccgtgg cgcgcagccg
    17401 cccgctggcg tcgtcgctgt tttcccagtc aatctcgaag tgctccccga tccacgcggt
    17461 caccgcgcgc acctggtccg aggtctggca gatgacgcgc ccgccgcaca ggtcggtgag
    17521 gcgtgccagg gggtcgggcg gcaggccggg cttcggcttg gagtagccgt cgcgtttgcg
    17581 caggattttt tccgcgaagc tcggcacctc cttggcgcgc acctggatga tggcctccac
    17641 ggccagtttc ttgcgcgcca tgttgagcac ggcggtcagc gcggcggcgc agctttcaaa
    17701 tcggggacgc agttccgtga aacgcggcgc gtgcagcgcc acccagtccg ggtctgagag
    17761 ccggggccag aggcccgggt cacggcggga ggtgtcatca agggggatgt gcttgccggc
    17821 gctcatgatg tcgtgctttt gcgcgcggcg tagtccagca tgaaggcgcg ctgggggccg
    17881 ggcagtttgt gttttttcag gcgggaaagt gaaacccacg cggcatcctc gtgctcgtcg
    17941 ctcaggcgga gcgcgccgcc gccggctcgc gcctccatgc acagggccac cacgcgcgcc
    18001 ctcggcatgt caaactccat cgcgcccgcc aggccggtga gttccagatt caggccgcac
    18061 tcctccagaa cctcgcgttc cagcccggcg ctgaagcttt cccccggatc cagcttgccg
    18121 ccaggccact cccagcagcc gggaaagtgc gtgctgccgc ccgcgcgccg cagcagcagg
    18181 cagcgtcccg agtcgtcgag gatcaccgct ttgacggaca gggaataggg cttggactcg
    18241 atcatggatt aaaaaggaaa tctggcgctg cgcatcgtcc aaaactccgg gccgcgtgtc
    18301 cagacagttt ttccaggaca gccatccctg acagtcctgt ttttttattc tgttcatcgc
    18361 tctgcggggc gggtattctg cccgcacaac ctctggaggc atttgatcat gacacaccgc
    18421 cgttcctttc tgacgtccgc cactggcgcg ggcgctctca ccgctttggg ccagcttgga
    18481 tttcttggac gcctgccgcg cgtctccgcc gcggaggcaa agctgccgcc gcatctggtg
    18541 cgttttcatc cggagatcga gccgctggtg cggctgctgg aggacacgcc gcgtgaacgt
    18601 gtgctggaag aggtcgcctc gcgtgtgcgg cgcgggctga gctaccgtga agtgctcgcc
    18661 gcgctcatgc tggcgggcat ccgcagcgtg cagccgcgcc cggtgggctt caaattccac
    18721 gcggtgctcg tggtgaactc cgcacatctc gccagtctcg cctcgccgga ctcagagcgc
    18781 tggctgccga tcttctgggc cattgaccag ttcaagtcct cgcaggccgc gaatgtgaag
    18841 gagggcaact ggctcatgaa accggtggac gagtccaggg tgccgtcgag cgaccgtgca
    18901 aagaaggctt tcatcgaggc gatggacaat tgggacgaaa gcgccgccga tgccgccatc
    18961 accggcctcg tgcgcacggc aggcgcgaac gagctgtttg aaatcttcgt ccgctacggc
    19021 atgcgcgact ttcgcgacat cggccacaag gcgatctatg tggcgaacag cttccgcacg
    19081 ctcgaagtca tcggctggca tcatgcggaa ccggtgctgc gctcgcttgc ctatgccttg
    19141 ctcgaccgcg cgggcgcgaa ggaaaatcct gccaaggccg atctgcccgc cgaccggcct
    19201 ttccgtcaga atctggagat cgtgaagcag gtgcgcgaag gctggctgga cggcaaacgc
    19261 gatgccggag cgaccaagga actgctgcaa gccgtgcgca ccggctcgcc cgccgacacc
    19321 agcgcggccg ccttgaagct gctgaacgcg ggcatcgcac cacagtccgt gtttgacgcg
    19381 ctttttgacg gcgcgggcga gctgctgatg caggcgccgg gcatcctttc gctgcatgcg
    19441 atgaccttca ccaacgccat ccactacgcg tggcatcaca cgcagagcga tgagacgcgc
    19501 cgcctgctgc tgctgcaaaa cgccgccttt ctcccgctct accgcggcga ccgcgcggac
    19561 aatggcgtcc acattgacgc gctcgaaccg atcacgccgg aggcgaaggg agaagaggcc
    19621 gtggcggaga ttttcgccga catcggcaaa gaccgcctca ccgccgcgcg caaggtgctg
    19681 ggctatctcc gggatcaaaa cagcgcgcag cccgtggccg atgccgcgcg acgcctcgtc
    19741 ttcctcaagg gcacgaactc gcacgactac aagttcagct ccgcggtgct ggaggactac
    19801 gagcacctcg cgcagccctg gcgcgaccgg ctgctggccg cgagcgtgtt ttacttccgc
    19861 ggctccggcg aaaaggacaa cgatctggtg ggccgcatcc gcgctgcgct gggcgcgtga
    19921 ggatttgcgg acaaggtttc gccccgcggc ccgtattcga tcccctccct cccgttgaac
    19981 accaccagcc actccacgga ccgccgcact ttttcaccgg accagcgcat ggtgctgtgg
    20041 gtgctatggc tgacctacgg cacgttctat ttcacccgga acaatctcag cgtggcgctg
    20101 ccgggcatcc aggaggagct gggctacacg aagtcgcaga tgggcacggt gctcatggtg
    20161 ctgaagctgg cgtatggcgc ggggcagttc atcaacgggc aactggcgga gcactggcag
    20221 ccgcgcaagc tgctcgcgct gggcctgctg gcctcggcgg cgctgaatgt ggtgttcggc
    20281 tggatgacgg cgctttattt catgaccttc gtctgggcct gcaacggcta tgtgcaggcg
    20341 ctgggctggc cgccgacgat gcgcgtggcg gcgaactggt tcccgcccct gcaacgcgga
    20401 cgtgccatcg gcatcatcgg cacaggctac cagctctgcg gcgcgctgac ctttgtcgtc
    20461 gcgggctggg cggcggagcg ttttggctgg cgcggcgcgc tgtgggtgcc ggcgctgctc
    20521 gtttcactga gcgcggtcca catgctggtc tttttgaagg agaaaccggc ggcggagcat
    20581 gccctggagg acattgagca cgcggcggcg atggcgaacc gctcgctgcg ggcgaatctg
    20641 ctggtgacgc tgggcaatcc cggcctgtgg ctcgtggcgg tggcgctgtg tctgcttgat
    20701 gcctgccgct atggcttcac cgactggggc gtgacacata tcaaagaggt gcagaatgcc
    20761 agcgtcagct cggcggcctt caaatacgcc gtgctgccct ttggcggcat cgcgggcgcg
    20821 tttatgtccg gctgggccac ggaccgtttt ttcaaaggaa ggcgcgcgcc ggtgatctgc
    20881 gtgctgatgc tcgcgctggg ccttttggcg ctcgcgtatg acacggtgat ccactggggc
    20941 atggcgcctt ccgtggccat tttgttcggc atcggattct gcatcttcgg cgcgcaggtt
    21001 ttgctcgtcg gcacgctgcc ggtcgatctc gcgcgtcacg gcacggcggc cgccgcggcg
    21061 ggttttgtga acttcatggg ctacatgggc gccgcggcgg gtgacaagct cacgggaagc
    21121 tttgccgagg tgcatggatg gcctgcggcg gtgcgttttt gggccgcgtg cgcatttgca
    21181 ggcggactcg tcatcgcctt cctctggaaa gccgctccct atcaccaggc agatctcaaa
    21241 cccgaacccc acaatcagaa ataatgaagg ctatcatcat cgcagccggc cgcggacgca
    21301 ggctcatgcc caccaccgcc gactcgccga aatgctatgc cgagattcat ggcaaacgca
    21361 tgctggacca cgcgctggat gcctttgcgg gcgcgggcgt cacggatgtg tgcttcatcg
    21421 gcggctatca gatcgacaag gtgcgcgccg actacccgcg cttcaccttc cgtcacaatc
    21481 aggactggga gaacaacaac attctgctca gcctcatgca cgcggaggat ctcatggacg
    21541 agggcttcat ctgctcctac tcggacatcc ttttcaccag cgatgtcgtg aagggcgtgc
    21601 tgcatcatcc cgccgacatc gctttgagcg tcgatactca atggctcgac cgctaccagc
    21661 accgctcaca acatccgccg gatgacgcgg agaaggtcat cgtgagcaac ggccgcgtca
    21721 cacgcgtgca tcgcggcatc ctgcccgatg aagcgtatgg cgagtacacc ggcatcgcga
    21781 aattcacgcc tgccggagcc gcgaagctgc gcgagcactt ccatcaggcg cgtgagcagt
    21841 tcgcaggcaa gcccttccgc gaggccgtcg tctttgagaa ggcgtatttg attctcctgc
    21901 ttcagcaaat gatcgaagcc ggcatcccca tggcccacag cgacacgccc ggccactaca
    21961 tggaggtgga cacacaggag gacttcgaac ttgcccgcag gcactggctg cgcgcgtgat
    22021 gcatttcaat tcctgcggct tcggaagtga ctgtggggat ggttatcttc gcccggaaga
    22081 agtcgtctcc ttgcttcacg atctgctctt tgatttcaag gagcgcagaa ctggctttgc
    22141 cccgcacctc gggggcgggg tgagtttcgt gagcctgaac agttgtctcc gtcatggaaa
    22201 cagttgccta aaccaccgag gaaaaggcat ttgccgcaag gcaaggcgct ttggagagga
    22261 ggcagagctt gttttttggg gattggcatg gctgggtggg gcttcaagcg gcggcacttt
    22321 gagaaaaacc cgcttcaaat ctcaagaaga cgggctttga aacctcatgg acgccgtgcc
    22381 cgccatcacc tccgccgtgg tggatcaggt ggagacactc gccgtccgag aggcgtccgt
    22441 ggtcaccgcc tccggggtga acggcgtgct ccacctcagc ttcggcatcc cccaagggtc
    22501 ccggatggag ccgaaggccc gcaaggcccg gtgtccgcgc aagacctcgc cgacggcctc
    22561 gacacccgcg cccagccatc actgcggttg agattcctga ctttagcgac ctttcactcg
    22621 cggaaagaag tggagtgcca taaaagtttt gtcgccgaat gccgtctcca atttcaacgg
    22681 caatttgagc actcttttcg aacgcgttac gcagtcgttc ggggtcatcg ttgttgtctg
    22741 gcagcttggt cagcgggttc tgttgaaagc ggttctcgaa ctcgatagtt tggttccaga
    22801 cttcctttgt gaacgactcg gagatgacgg cccaatcgtg cgacggcgtt attatcacgc
    22861 gcttgcgtag aagaagaaac accagcaaga cgataattaa aagtccttcg atgaagatgg
    22921 caatttgcat cggttttcgt tatggggtgc gtgcaaagtc acatgaaatt acactgatac
    22981 acaaaagtat tggtgaatag tatttgaaga aagtcttgtg cgcatgagga agatttatcc
    23041 acaatgacag gattgcgctg cttgttggtg actgagaagg tcgggccctc acacccgcag
    23101 gcaggcgcgg aagccgcgcg agctgtagta ggattcggcg ccgttgtggt agaagaagac
    23161 gcggccgaag cgggtgtcgc caaagagggc gccgccgagt tttctcatgc cgggcggggt
    23221 gagcacccag cttgaggttt tggtgtcgaa gggtccgagc tgctggaggg cgagatactg
    23281 ctgctcggac aaaacctcca cgcccatgcc ggcggcgaga tcgagcgcgc tgccggcggg
    23341 cttgtgctcc ttgcgcgcgt ccagcgcggc gcggtcataa cacaggctgc ggcggccttt
    23401 ggggctttcg ggggagcaat cgcagaagag gatttcgcct gtcgcctcgt catgcccggt
    23461 cacatccggc tcgccgccgg ttctttccat ttcagccagg gaccacagcc tgtcaggcct
    23521 ggcttcgagc ctttgccgga ccgcctccca ttcgatgcct ggatggcggt gcgggttttg
    23581 ggcgaagcgc tcttgcagga cgttgagaaa agcgctgcgc tcttttttcg gaagttcctt
    23641 tttcatgggg tgataaaccc ctgtttcgaa aagaacgcat ccatcttttt cagtgtctcc
    23701 gcatgaagcc gggcgtcctt gaacatgcag gtgtggacgg ctccgggtgg tgagacaaac
    23761 tcaatgtgat tgacctggcg cgttggtctc attccttgcg caagccgggt gcataggctg
    23821 gcagtggcct ggccgtggcg gcggtggttg ttttgcccgc agccgtcttc gcgaggttcg
    23881 tcctggtggg cagtcgcgcc agagcatctt cacggactgt caaatgcagc acccgctgcc
    23941 agcgg
//
LOCUS       DAOMYJ010000060        23717 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Prosthecobacter sp. isolate AMR_MDS_5629
            NODE_767_length_23717_cov_22.781464, whole genome shotgun sequence.
ACCESSION   DAOMYJ010000060 DAOMYJ010000000
VERSION     DAOMYJ010000060.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867850
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prosthecobacter sp. (wastewater metagenome)
  ORGANISM  Prosthecobacter sp.
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Verrucomicrobiaceae; Prosthecobacter.
REFERENCE   1  (bases 1 to 23717)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 23717)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.30
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:34:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,742
            CDSs (total)                      :: 4,684
            Genes (coding)                    :: 4,676
            CDSs (with protein)               :: 4,676
            Genes (RNA)                       :: 58
            tRNAs                             :: 55
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23717
                     /organism="Prosthecobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_767_length_23717_cov_22.781464"
                     /isolate="AMR_MDS_5629"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1965333"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            173..1057
                     /locus_tag="PLP58_08415"
     CDS             173..1057
                     /locus_tag="PLP58_08415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ08576.1"
                     /translation="MSPATPAEKPPAQGGPRHTLMAGVALLFTAQLSVLLPHGDSGEA
                     LREGLLANGWFTRAMLALWGVIGLEAVLGLFSAPDAWSARIKRLLIVCAAPPLRMTLA
                     TSTPPGWLWVPRFGWQPAGPGTTLKLERRLAIPMIVLTLLVVPVLVAELGFGETLDNH
                     PRLAEAVHYLTSLIWLGFAAEFIWMVSATTEKMAYCLRHWVNLLIILLPLVAFLRFLR
                     LFRFARMFKAGKLLRAYRLRGLWMRVWRTALLFNLIERLQERNPEKYRVMLEKKIREL
                     EAEVERLREKLALLKDRE"
     gene            complement(1083..1625)
                     /locus_tag="PLP58_08420"
     CDS             complement(1083..1625)
                     /locus_tag="PLP58_08420"
                     /inference="COORDINATES: protein motif:HMM:NF016214.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HRJ08577.1"
                     /translation="MSPVGHSITGLALAALALPGKAPRRWLGGAAFIAIANLPDWPLP
                     GWGHDRYEISHSLFVNLALITTGALVWRSIPALRQALTARLILLAACAWLSHLLLDSF
                     YNHGKGLGIGWPLLEYRLNLPVPFFRIVDTSLALWNPRNLSVFAIELLAYSPLLALAL
                     RHRCGCRCLRHQGSAQRDMP"
     gene            complement(1644..1976)
                     /locus_tag="PLP58_08425"
     CDS             complement(1644..1976)
                     /locus_tag="PLP58_08425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ08578.1"
                     /translation="METELEIPPIKPFLDLKLRDDKIIYRRGRSTFRVRVEELHAAYA
                     HHRGQRITTNAIRQFKPAVFDSKARPAGHSCNISLLFSLLVRLELAESLTGKGSRGDP
                     FTLRIKDA"
     gene            complement(2227..2949)
                     /locus_tag="PLP58_08430"
     CDS             complement(2227..2949)
                     /locus_tag="PLP58_08430"
                     /inference="COORDINATES: protein motif:HMM:NF013921.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - transposition, DNA-mediated
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HRJ08579.1"
                     /translation="MTWNIPSVIPLPENFTGFDPSSAIPPENFTSYERHLPHWRIPGA
                     CYFTTFRLRDSIPKAVLEKMRREDLEWKQRLAKSSAQQGGMPSPVEFQAWQEFKRARL
                     RKLELLLDEGRGECLLRVPAYREIVEKALRHFEGDRHEMLAWTVMPNHVHALLRPLHE
                     HLLEDLCGSWKWFTASKIQAALQRTGPLWQEENFDRIIRDAAHYEKAVRYIANNPVKA
                     NLPPGQASAGFCQSIRAANGWP"
     gene            complement(3571..5118)
                     /locus_tag="PLP58_08435"
     CDS             complement(3571..5118)
                     /locus_tag="PLP58_08435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009965101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arylsulfatase"
                     /protein_id="HRJ08580.1"
                     /translation="MKTPFLFLLLALALTARAADKPNFIFILCDDLGYGDVGCLNPEG
                     KIPTPNMDRLAREGVIFTDAHTSSSVCTPTRYGVMTGRYNWRSKLQKAVLGGLSPRLI
                     EQGRMTVASMLQGQGYHTACIGKWHLGMDWALHPGKTVSELTIESREQAFSADFTRPA
                     TNGPNAVGFDHYFGISASLDMVPYCFIENDRVTAQPTEDRGLPMFHGEDKERNSRQGP
                     AAPGFTGYEVLPTLTDKTVAYIGEKAADAKAGKPFFIYLPYASPHTPILPNERWRGKS
                     GLNSYGDFVMEQDHEIGRVLKALDDHGLTENTLIVFTSDNGCSPQARYDELLAKGHNP
                     SGKFRGHKADIYEGGHRVPFIVRWPAQVKGGQTTAQLTCLTDFTATAAEILGVKLPDT
                     AAEDSYSFLPALTGAASASPVRRSVIHHSIGGYFAIREGKWKLALCPGSGGWSEPRPG
                     KEPRDAPPVQLFDLETDIGETNNVAAANEDIVKCLQMKLEEIVANGRSTPGARQKNAV
                     PVDIWKK"
     gene            5430..13760
                     /locus_tag="PLP58_08440"
     CDS             5430..13760
                     /locus_tag="PLP58_08440"
                     /inference="COORDINATES: protein motif:HMM:NF025264.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="immunoglobulin domain-containing protein"
                     /protein_id="HRJ08581.1"
                     /translation="MKLPALSSSRSAYFKLVPTLMLALALASASAQTVAFTEKFETSQ
                     GDAYTTSGVIGASVWTVTRSTAGDWGARIHNAQLELTNDAGATGNANGWVLASTPTTS
                     FGLPYNSTLSSNPGAVTWEFNMRQIRPDPAGFSAGSYGVAFVLAGSSANAATEGSGYA
                     VVLGQSGATDPVRLVAYVNGLQGTLTTIITATAPLGDVGVDYMSLRVQYGPGSNIWEL
                     YGRPDGTTAFSDPALGDLTLLGSGTNGTHTATSLGFLGGYWQGSTAATQTAFFDNIRV
                     LVLEPVPVSQLFWDANGNTAGAGGATPAGTWGVDAFWSSDLNGETATGAWVPAQRAVF
                     AAGTDASGEYTVTLSGAQDVNGIHFEEGHAQLSGGALLLGSSTPFVIVDAPQATIASD
                     ITGTNGLIKNGAGALNLAGVKTFSGSVVINAGVARIAEDGALGAATNDVGLFGTLATT
                     QSLTLGAGRDFTGGGTLAPAAGTTAELAGGVTMTGLTLADTGTVNLTGVTRSVGALSF
                     TVAGILTGSGSITASGLSAAALTSGTAMVGAPLDFTPAGTTNMTVNVGAGGQLTLGGA
                     LTKLSSGSAGRLSKQGAGTLRLEGANVDLCGVQLGVVGASPVVGGTLVITNKDALGTR
                     DTDTVAAGAQYFQAQLNSGTLQIAGTLTGGNAVPNGISIGARAASPLVFTGGDVEFLG
                     ASSLFGATGTGDDIVVEVNNHVTLPGAFTQGGGGAITGLALSGTGTLTFANAATLLTS
                     PLKLGGTVTVELDTATLGATTAAAPVHSLAAGTTLVIGKPGETRLVTAFGGLDGAAGS
                     KIAFDLDGLARGDDYDALVLAKPDGAAGAVAFAGTIEVELVGAFEPEIGDTFDLLDWD
                     ASVTPDFTGIAFDLPPLPMGREWNTNSFAANGSISVQAESNVDFTITTQPQSQTKLVG
                     QPVTFTVIITGTGPFSYQWRKGAVNQGGPTSDNFLTIGAVSPADAGTYFVDITNASGT
                     LGSAGAVLTVITPVQITQQPAPATQTVIEGATVQITAAATGTGPLSYNWRKGTDSLGA
                     PNDPVLTLNNVQIADSGDYNVIVTGPGMDNFEVSQNASLTVNPAPPPGPISITGNMTY
                     SENFDGMGTTTPGTEFPNGWKGFKFAGTGGMAPGTELTNLTTPAFTASNGGGTAGTLY
                     NYGVEGANPLADRALGSQASGGFIGAFGVSFINDTGGPLEGVNIKIGFRSELWRTGNN
                     AGEERWIFEWKLGGGITDLDLGEWNTASAFDIVEIDPTNTTNSSRDGNAGGNFATLSP
                     AFFSSLSGWEPGQVLHIRWRDVDNTGNDAGMAIDDFTFTAENVPPPPTLLYWDANGAL
                     AGAGGSAPTGTWGVDTFWGGIADGTGATAAWQAGGDAIFSAGADATGMFTVTVAGTQD
                     LGALVFEEGQVTLSGGTLNFTDITPKVEVLDTGSASISSLLTGATGLLKRGGGILSLT
                     NPANSFTGNVVLGGGILAVTDDAQLGNADNDIVLSGGSLLTSVSLSLNAQRSISGSGG
                     LAPDAGTTLTLPGPVATSALSVTGTGTLDFTGASPTLGSLSFQGAAASTGVQLALSSI
                     GANHSSGTSIVSNDLNFGTTNATAEVATGGTLQLDGQLSLGGGGSNRLIKTGGGILII
                     SGAQPGLNKLGIGIQQASPIEGGVVVINDKEGAGATQSFFNYGTLQAAVPLTGDEAIA
                     IGLSIGGRDASPSVLAGEEMTFLGDSTLFAATGTMGDIRLNVNNHTTLSGLLTGSLNA
                     GLSGFAIGGNGMLTLNNVLSNLTTGIKLKDTVTVELAPASSLGTAGMTSAPMVGIDAG
                     ATLAVGLIGAVQEVIAYAGLQGSAGSVLRFDIGGAVRGTAYDALRLLKLDNDQAGAVI
                     FAGQVKADLISGFAPALGQSFDLLDWDAGVTPDFTGISFDLPPLGAGLGWNTSYFAVD
                     GTIRVVTDVLTILEEPDSASVSPGQAVSFSVVASGPGIISYQWQKLVGMDFENVVTNG
                     NEATLLLLNPTTDDAGVYRCLVSNGGAPIESAQATLIVFPPLTDVMATRTPAANSYFI
                     IGDSVTFNVTFNNPGGGTVTYQWYRGSSPIGGATGPSFQINPLSLASTGTYSVRVSTG
                     GPPISSNGVPIVVVSPEPAIATQPAPRTLVAVGQPLTLFVEGTGRPPLRYQWKRDGKV
                     IPGATKASLVLPKAALVHAGTYICEVTNAAGPLVTSSTAQVGVVTVNASPPKLNLPEK
                     GKSVMKVLAAGPNLLFNWTRNGGAVPANFISTPDGKTLNGSNLTINDHGSYACTVTLG
                     VLTLQGGQHLLQVYDSEPDSPATTTMPDGIIGGNYIFDMPYTVGDPPTEAPDSFSAKG
                     LPAGLKMDPKTGRISGRPTKAVTSVPVEMTAKNKLGSTKTTSTLTIHAFPAKLAGPFT
                     ALVERDPDLYGNLGGRLDMTITTLGAYSGSLLMGTTKFAIKGVLDLDALDPNGLDPDE
                     GVPPGFEIVIPQKNSPAPLILELDMPLLDEDGDPDINGERFVTGTVTDGTGTADIDGW
                     WQKWNPKAGAAASDFQGYYTFGLAIPENDTALGDASVPQGAGYASFTLAADGKYKLAG
                     KTPDGESLTTAGISGPAGQILVFQTFYKPAAGSLLGVPVISTTGGATPQDRPLMGDAD
                     WLRPENTNLKHRVFRAGFALQPMSIMGGRYSLPDAGNVVMNLAPDSQVTMSFLEGGIG
                     TTFDGPPGQTSRVDMTLRIDAKNKLITVAGPALPDTNKPKPIAPKTGLFGGSIGLQDG
                     NPVPNGKPNPVKRTAAYQGILIRDGSEYIGVGYFLLPNLPTDAVNPPTTPTTSPIESG
                     MVLIEP"
     gene            complement(13815..14987)
                     /locus_tag="PLP58_08445"
     CDS             complement(13815..14987)
                     /locus_tag="PLP58_08445"
                     /inference="COORDINATES: protein motif:HMM:NF013434.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HRJ08582.1"
                     /translation="MEKQALILGGGVFGLAAALELRTRGWRVVLLDAGGIPHPDAAST
                     DISKVVRMDYGADPLYTAMGEAAIRRWREWNARWGEDLFHEDGFLVMTRRPMTPGSFE
                     HDSHAFLTARGHPLRRVGAETLRRAHPKWNAGAFADGYFNPQGGWAESGRVVARLKLD
                     ALAAGAEIHEHRPFHRWLEEGGRVCGVMDAHGAEWRADVVVSALGAWTPEHLPWLADR
                     LHATAQSVFHFLPQNAAEWRAPAFPVWAADIGTTGWYGFPANANGVVKVANHGPGRRV
                     RASDARMMPDGEEERFRAFLRATFPALADAPVAASRVCLYGDSFDGHFLLDHDPGHPG
                     LFVAAGDSGHAFKFTPVLGPLIADAVEGRPNPWLERFRWRGLGGTDGEKARAGGNK"
     gene            15131..17860
                     /locus_tag="PLP58_08450"
     CDS             15131..17860
                     /locus_tag="PLP58_08450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009960714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-related protein"
                     /protein_id="HRJ08583.1"
                     /translation="MEQALPGQRWVSDSEPELGLGVVMKAEFGRVEIYFPAANEHRQY
                     AIKTAPLRRVRFQEGDTIKTHEGQSLEVESVEERKGMLFYLGASRQVSEAELSDTISF
                     SKPEDRLLAGQVDEPREFDLRVEALKRRAALRRSTVRGFCGGRVDLLPHQMYIASEVS
                     SRLVPRVLLADEVGLGKTIEASLILHRLHLTGRAERVLILVPEPLVNQWFVELFRRFH
                     LLFAIFDEMRCDSIETNEENGNPFLDSQLVLCSTAFLAGSPERARQAAEAGWDLLIVD
                     EAHHLEWTPERASPAYTLVQSLAEKTPGLLLLTATPQQLGPEGHFARLRLLDPSRYAD
                     LGQFLEETLHYEEVARALDRLLAGKSPTAADSALFIKKSPHLQEHLDAMKAGTEGARE
                     KLVSSLLDEFGTGRVMFRNSRAALSGFPQRSAKIIPLEAADDPLDAKVKWLAGLLKKL
                     GGDKVLLICRSRDLAVEIHEKLLHEVNLNAAVFHEGLTLLQRDRQAAWFAEEEGARVL
                     ISSEIGSEGRNFQFAHHLVLFDLPDDPELLEQRIGRLDRIGQSSTIHIHAPCIQGGPE
                     EVLARWYHEGLHALEANLHGATEILHALRPELDALLAAPEPDRLEAFIQQSGELRKTV
                     AKKLERGHDRLLELNSCKPEKAAQVIDDIRAQDRDTEFEEFFIRLVDHFGMHVEELGN
                     RSYFLLPGNLTTDAFPALPDDGLTVTFDRARALSRENTGFLTPDHPMVRGALDLLLGA
                     EGGNSSFGLWRGSGSEGLMLETHFVVECIAPPELHVDRFLSPGPLRVVVDHAKKDRRD
                     DAAFMHARIEKGSPSRLFENPSIKRKIFPAMLSKSRKIAKAEMEALVEAAVSSMRAQL
                     QAEIERLEDLAQINDHVRPEEIEAMRSHMALLEKALGSARLRLDALRLVLRMS"
     gene            complement(17966..19309)
                     /locus_tag="PLP58_08455"
     CDS             complement(17966..19309)
                     /locus_tag="PLP58_08455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009966024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HRJ08584.1"
                     /translation="MSQNNRRSFLKTAAGAVSAPFILPSRVWSAETAPNSRVNLGFIG
                     MGKMNSGHLGNFLGRENCQVVAVCDVDTTRREHAKGRVDETYGKKADGAYKGCAAYSD
                     FRELLARKDIDAVVVATPDHWHAYIGIAAVKAGKDVYGEKPLTHNVHEALLLTKAVRD
                     SGRIFQTGSQQRSSKEFRVACELVRNGVIGQIKTITTSFGDPAGPYNLGEEPAEPGLD
                     WDMWCGPGPLKPYNSILSPRGVHNHFPAWRMTREFGGGMITDWGAHHIDIAQWGLGVD
                     DSGPVEVRAPQGGADAKRGAQLVYANGVVLTHGEGKGVSFYGTEGEVHVNRGKFELWL
                     GGRKVHGFYDREQDKGTSLDREVVLSEREYLKDAKVRLYESKNHHDDWLNSIQTRKPP
                     ICDVAIGATTVISCHLMNMAYWHGANFKWNPVERAFAEGGDPKWLTRDYRGGWAV"
     gene            complement(19459..20946)
                     /locus_tag="PLP58_08460"
     CDS             complement(19459..20946)
                     /locus_tag="PLP58_08460"
                     /inference="COORDINATES: protein motif:HMM:NF013567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HRJ08585.1"
                     /translation="MNPTNTSRRAFMRGTLAGAAGLATLPAWAKPIGANGDVRVAVIG
                     FKSRGNGHISSLLKIPGVRLAALCDVDSEVMDKKVAELAKKDIKVKQYKDFRECCADP
                     DIDAITIATPNHSHTLIALTGLANGKHVYVEKPVSHNIWEGRKLVEAAAIAARKGLVT
                     QHGMQRRSDLGWAAAMEWVKAGHIGKVTLSRGINFKARQSIGKVDAPVAPPATVDYKL
                     WSAPRTELPVNRAKFHYDWHWQWAYGNGDIGNQGPHQLDVARWALGNPDKLPVRLMSF
                     GGRWGYDDDGQTANNQMAYYDYPGNAPLLFDNRGLPMKDMNWTKGFEPVFRINGKTSA
                     PRIGNVIHCEGGFVAESKAYDNDGKAIEKFDNFQDGPDHMLNFIESVRAGKITKDVLH
                     VAHGHHAAALAHLANVSYRLGKKLGTGEVKERLSASKPAQETLSDFFANLEANQVDLS
                     KDLPAVGPWLEFDPESEKFVGEFADEANKIAMEEYASGFELPVIS"
     gene            complement(21112..>23717)
                     /locus_tag="PLP58_08465"
     CDS             complement(21112..>23717)
                     /locus_tag="PLP58_08465"
                     /inference="COORDINATES: protein motif:HMM:NF024116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="lipocalin-like domain-containing protein"
                     /protein_id="HRJ08586.1"
                     /translation="IRASTRRLSVSVAALAVSLALTVAIAVMVGSFRQTVLLWVDQSM
                     GADLYVRPGTPARSQGAPTLSQQTLDLLTGHPAVAAADGYRALDLPYQGRIVKLGTGD
                     FAVQVEHGRMVFKTPGDPKALLQRAREKGQVFISESFALRFGVREGGSVELATPQGPR
                     TFTVAGQFYDYSSDGGTLTLDQALFEKHFGASRPTHLALYLKPGSDAGAVREEMLASL
                     DGRGFVSVFTNAGLRAEVLRIFDGTFAITWALEIIAVLVAMAGVAATMLTLVLERREE
                     LRLLRLAGAEAGQVRRTILIESGLIGAVSQALGLVVGALLSLVLIHVINPQSFGWSIQ
                     FHTPWAFLLGSTVLTVLCTMLAGLWPARRAVKKLALGGMAVLLLSGNAQAASTPAPMS
                     NRAVWEPLGTKPTSFVTAKEPFETCQKPFGTGQKAFETCQKPFGTGQKAFVACQKPFG
                     TGQKAFVTCQKPFGTGQKAFVTCQKPFGTHQKLFGTGSEPRQNLQESPPEAAPAPESG
                     FEQAGGGPAAPPAAPPWQPAQPGYRYEFPRDHAQHPRHKIEWWYFTGNLDTREGRRFG
                     YQLTFFRIGAVAQPAVQSPWALRDVWMAHLAVTDAQGRAYHHADRLNRTGPGLAGSTQ
                     ERVWNEDWQCLFEPGGMRLRAAEGAFGLELELEKAKPPVFHGRDGISQKGPTPGNASH
                     YYSFTRLPTRGTVTMDGARHEVAGLSWMDHEFGSSFLEKGTTGWDWFSAQLDDGSELM
                     LFQLRGENGGATTGSAGTFIHPDGSAEALAADDLRLTPGQMWRSPAGARYPTGWSLEV
                     PRLGLRLESYAVLPQQEFQAAATPGLGYWEGAVDYRGTRAGKAVRGRGYLEMTGYSGR
                     SMSLWFGGDGE"
BASE COUNT         4078 a         7562 c         7866 g         4211 t
ORIGIN      
        1 tcgagccagc gctgatacca gcgggcttcg acctcggctg gtgtgtaggt tttgtcgagt
       61 tcgggcataa agggccgcca agatgggggc gggcgcgcgt tttgcaatgc cggggaggac
      121 gcatcctgcc atcttgcgcc ggaggagaaa acatgccaca aaggcagccg tcatgagccc
      181 ggccacaccc gcagaaaaac cgcccgcaca aggcggcccg cgacacacgc tgatggcggg
      241 agtcgcgctg ctgttcacgg cccagctaag cgtgctgctt ccgcacgggg acagcggcga
      301 ggctttgcgg gaggggcttc tggccaatgg ctggttcacg cgcgcgatgc tcgcgctgtg
      361 gggcgtcatc ggcctggagg ctgtgctggg gcttttttcc gcgccggacg catggagcgc
      421 gcggatcaaa aggctgctga tcgtgtgcgc cgcgccgccg ctgcgcatga cgctggccac
      481 gagcactccg cccggctggc tctgggtgcc gcgctttggc tggcagccgg ctggtccagg
      541 cacgaccctg aagctggagc ggcggctggc catcccgatg atcgtgctga cgctgctggt
      601 ggtgccagtg ctggtggccg agctgggctt tggcgagact ctggacaatc atccgcgtct
      661 ggcggaggcg gtgcattatc tgacgagcct gatctggctg ggcttcgcgg cggagttcat
      721 ctggatggtg tccgccacga ctgagaagat ggcctactgt ctgcgacact gggtgaacct
      781 gctcatcatc ctgctgccgc tggtggcttt tctgcggttc ctgcgcctgt ttcgtttcgc
      841 gcgcatgttc aaggcgggga agctgctgcg tgcctaccgc ctgcgcggcc tgtggatgcg
      901 cgtgtggcgc accgcgctgc tgttcaatct catcgagcgg ctccaggagc ggaaccccga
      961 gaaataccgg gtcatgctgg aaaagaagat ccgcgaactg gaagcggagg tggagcggct
     1021 gagggaaaag ctggcgctgc tcaaggacag ggaatgaagg tcttgaaatc gctgccgatg
     1081 actcaaggca tgtcacgctg cgcgctcccc tgatgccgca ggcagcgaca tccgcagcgg
     1141 tgccggaggg cgagcgcgag caggggcgaa taagcaagca gctcgatggc gaagacgctg
     1201 agattccgcg ggttccagag cgccaggctg gtgtccacga tgcggaagaa gggcacgggc
     1261 aggttgaggc ggtattcaag gaggggccag ccgatgccga ggcctttgcc gtgattgtag
     1321 aaggagtcga gcagcaggtg gctgagccag gcgcaggcgg ccaggaggat gaggcgcgcg
     1381 gtcagggcct ggcgcagggc ggggatggag cgccagacga gcgcgcccgt ggtgatgagg
     1441 gccagattca cgaaaaggct gtggctgatc tcataacggt catgccccca gccgggcagc
     1501 ggccagtccg gcaaattggc gatggcgatg aaggcggcgc cgcccagcca ccgtcgcggt
     1561 gctttccccg gcagcgccag cgcggcaagc gcgagcccgg tgatgctgtg gccgacgggg
     1621 gacatgcggg gggataaact gcgtcaggcg tctttgatcc gaagagtgaa tggatcacct
     1681 cttgagcctt tgccggtgag ggattcggcg agttcaagtc tgaccaaaag tgaaaagaga
     1741 agtgagatat tgcaggagtg tccagcgggg cgtgctttgg aatcaaagac ggcgggtttg
     1801 aactggcgga tggcgtttgt ggtgattcgt tgtccccgat ggtgagcata ggcagcgtgc
     1861 aactcctcca ccctgacccg gaaggttgat ctgccacggc ggtagatgat tttgtcgtcc
     1921 cgcagtttca aatccagaaa gggtttgatg ggcggaatct ccagctcggt ttccatgtcc
     1981 aggaaactgg atatgcggtt ggtaaaggct tgccgggtca tgaacgcatt ccaaacagga
     2041 ttggctcagt gatgccagga ttttgttgat gcgcgcgagt ttttcgcggc tgccggtagg
     2101 ccgggtgtgg ctttagcccg cccggctatg cgcgcgttgg gtgcgagatt cccgcgccaa
     2161 gagccgggcg gtccggcggg ggaacggatt ggcgaaagca tgcccatagc gccggacaca
     2221 cccggcctac ggccagccat tggcggcgcg gatgctttgg caaaagcccg cggaagcctg
     2281 gcccgggggc aggttggctt tcacggggtt gttggcgatg tagcggacgg ctttctcata
     2341 atgcgcggca tcccggatga tgcggtcgaa gttttcctcc tgccagagtg ggccggtgcg
     2401 ctgcaaggct gcttgaatct tgctggccgt gaaccatttc caggaaccgc acaagtcttc
     2461 caggaggtgc tcatgaagcg gtcgcaaaag cgcgtgcaca tggttgggca tgacggtcca
     2521 ggccagcatt tcgtggcgat ccccttcgaa gtgccgcaag gctttctcga cgatttcgcg
     2581 gtatgcgggc acgcgcagaa gacactcgcc acgtccttcg tcgagaagga gttccaattt
     2641 gcgcaggcgg gcgcgtttga attcctgcca agcctggaat tccacgggtg acggcatgcc
     2701 tccctgctgt gcggaggatt ttgccaggcg ctgcttccat tccagatctt cgcggcgcat
     2761 tttttccaga acggctttcg gaatggagtc gcgcagacgg aaggtggtga aatagcatgc
     2821 gcccggtatc cgccagtgcg gcaggtggcg ctcgtagctg gtgaaatttt ccggcggaat
     2881 cgctgacgat ggatcaaagc ctgtgaaatt ttccggcaga gggatgacgg agggaatatt
     2941 ccaggtcatg acgggcgtcg gtcgcggttt cgatgggagc gcgggaatgg aagtttcaag
     3001 acccagcgaa tgcaagctgt gactgcacgg cttcccgcca aaagccgggc gggctaaagc
     3061 cacacccggc ctacggcagg gcgctgttct ctactcattt accaaatcat caaaaaacaa
     3121 agttagcggt aaatattcca ctcttcgtga agaatcgcag tgttgttttg ctgtatttga
     3181 acattggttc gcggagctga gttgatgcgc gtccaagctt ccgccacgtt gaagttcgaa
     3241 ctcggccgca aatggtctgt gtaatagccg gtcactcggc cattctccaa gccgacataa
     3301 gaccacatgc ccgcgccttt gccgtcccaa aaattgtccc attcataagc taacaattcc
     3361 ctgccttgag atgcggcgac gtgagttggg cgtccaattt ttcgaccact tcctgctttg
     3421 tcatgccaac tgagattcga ttgaagtcag gggccgcttt ccaaacgccc atgcagccag
     3481 tgaggttagc tgcgaccgat aaaacaatga gggttgagag tgcagaactg cgcatgatcg
     3541 agtttggcga tgatgaatcc cgtattgcac tcactttttc cagatgtcca cggggacggc
     3601 gtttttctgg cgggcgccgg gggtgctgcg gccgttggcg acgatttcct caagcttcat
     3661 ttgaaggcac ttcacgatgt cctcgttggc ggcggcgacg ttgtttgttt cgccgatgtc
     3721 ggtctcgagg tcgaagagct gcacgggcgg ggcgtccctg ggctccttgc cggggcgggg
     3781 ctcgctccag ccgccggagc cggggcagag ggcgagcttc catttgccct cacggatggc
     3841 gaagtagccg ccgatgctgt ggtggatgac gctgcggcgg acggggctgg cgctggcggc
     3901 gccggtgagg gcgggcagga agctgtagct gtcttcggcg gcggtgtccg ggagcttgac
     3961 gccgaggatc tcggcggcgg tggcggtgaa gtcggtgagg caggtgagct gggctgtggt
     4021 ctggccgcct ttcacctggg cgggccagcg tacgatgaag ggcacgcggt ggccgccttc
     4081 gtagatgtcg gctttgtggc cgcggaattt gccgctgggg ttgtggcctt tggcgagcag
     4141 ctcgtcgtag cgcgcctggg gggagcagcc gttgtcgctg gtgaagacga tgagggtgtt
     4201 ttcggtgagg ccgtggtcgt cgagggcttt taagacccgg ccgatctcgt ggtcctgctc
     4261 catgacgaag tcgccgtagg agttgagtcc gcttttgccg cgccagcgct cgttcggcag
     4321 gatgggggtg tgcgggctgg cgtagggcag gtagatgaag aagggtttgc cggctttggc
     4381 gtcggcggct ttttcgccga tgtaggccac cgttttatcg gtgagggtgg ggaggacttc
     4441 gtagccggtg aagccggggg cggcgggtcc ttggcgggag tttcgttctt tgtcctcgcc
     4501 gtggaacatg ggcaggccgc ggtcctcggt gggctgggcg gtgacgcggt cgttttcgat
     4561 gaagcagtag ggcaccatgt cgagggaggc gctgatgccg aagtagtggt caaatccgac
     4621 ggcgttgggg ccgttggtgg cgggccgggt gaagtcggcg ctgaaggcct gctcgcggct
     4681 ttcgatggtc agttcgctga cggtcttgcc ggggtgcagc gcccagtcca tgccgaggtg
     4741 ccatttgccg atgcaggcgg tgtggtagcc ctggccctgg agcatggagg ccacggtcat
     4801 gcgcccctgc tcgatgaggc gcggactcag gccgccgagc acggcttttt gcagtttgct
     4861 gcgccagttg tagcggcccg tcatgacacc gtagcgcgtg ggtgtgcaga cggacgagga
     4921 ggtgtgcgcg tcggtgaaga tgacgccctc gcgcgccagt cggtccatgt tgggcgtggg
     4981 gatttttccc tcggggttca ggcagcccac atcgccgtag ccgaggtcgt cgcacaggat
     5041 gaagatgaaa ttcggcttgt cggcggcgcg cgcggttagg gcgagcgcga ggaggaggaa
     5101 aaggaatggg gttttcatgg gttggctgcc gctgcgaggt cggcgtgtgt ctgggaatgg
     5161 aaaaaacagg cgcgaaaaaa aacgggcgga ggcaaacgcg gggttgggga gcgactttgc
     5221 aggcatcgga aagtgttggg agcaaagttg cggccggttt ttcaaccttc gtcatctgtc
     5281 ggaattacca gaattacagc gcttgatgct ggcatgatcc tggctgggtt ggcggagtct
     5341 gtgtcgtttt tgtttccaac cttgaccgtc cgtgcgcctt cctggcgtgt agcatgtcgc
     5401 gtaaatacat tgtcaacctt gccgccgcca tgaaattgcc cgccctctcc tcctcacgct
     5461 ccgcttattt caagctcgtt ccgaccctca tgctggcgct ggcactggct tccgcctcgg
     5521 cgcaaacggt ggccttcacg gagaaattcg agaccagcca gggtgacgct tacaccacca
     5581 gcggtgtcat cggcgcgagt gtgtggacgg tgacgcgctc gactgcggga gactggggcg
     5641 cgcggattca caacgcgcag cttgagctga cgaatgacgc cggcgccaca ggcaatgcga
     5701 acggctgggt tttggcctcg acaccgacga ccagcttcgg gctgccctac aactccacgc
     5761 tatcgagcaa tcccggcgcg gtgacgtggg agttcaacat gcggcagatc cgacccgacc
     5821 cggcgggctt tagcgcaggc agctatgggg tcgctttcgt tttggcgggc agcagtgcca
     5881 atgcagcgac agagggcagc ggttatgcgg tggttctggg ccagtctggc gccaccgacc
     5941 cggttcgtct tgtcgcttat gtgaacggac tgcaaggcac gctgaccacc atcattaccg
     6001 ccacggcacc cctcggcgat gtgggagtgg attacatgag tttgcgcgtt caatacggcc
     6061 cgggcagcaa catctgggag ttgtatggac gcccggacgg cacgacggcc ttcagcgatc
     6121 ccgcgctggg cgacctgacg ctgctcggca gcggcaccaa cggcacgcac acagccacat
     6181 cactcggctt tttgggcggc tactggcagg gttcaacggc cgccactcag accgcctttt
     6241 tcgacaacat ccgcgtgctg gtgctggagc cggtgccggt gtcgcagctt ttctgggacg
     6301 ccaatggaaa cacggcgggg gcgggtggcg ccacgccggc tgggacctgg ggtgtggatg
     6361 ccttctggtc ttccgacttg aacggcgaga cggccacagg agcctgggtg ccggcgcagc
     6421 gggcggtgtt cgcggcgggc acggatgcct cgggggaata cacggtgacg ctttccggcg
     6481 cgcaggatgt gaacggcatc catttcgagg agggccatgc gcagctcagc ggcggggcgc
     6541 ttttgctcgg ctcctccacg ccttttgtca tcgtggacgc gccgcaggcg accatcgcct
     6601 ccgacatcac gggcaccaac ggattgatca aaaatggcgc gggtgccttg aatctggcgg
     6661 gtgtgaaaac tttcagcggc agtgtggtca tcaacgcggg cgtggcgcgc atcgctgagg
     6721 acggcgcgct gggcgcggcg accaatgacg tcggtctgtt cggcaccctg gccaccacgc
     6781 aaagcctcac tctgggcgcg gggcgtgatt tcacgggggg cggcactctg gctccggcgg
     6841 cggggacgac ggcggaactg gcgggcggag tgacgatgac gggtctcacc ctcgcggaca
     6901 cgggcacggt gaatctgact ggtgtcacgc gctcggtggg cgcgctgagc ttcacggtgg
     6961 cgggcatttt gacggggagc ggcagcatca cggcctcagg gctgagcgcg gcggctttga
     7021 ccagcggcac ggcgatggtg ggcgcgccgc tggatttcac cccggcaggc accacgaaca
     7081 tgacggtgaa tgtgggcgcg ggcgggcagc tcacgctggg cggggctttg acgaaacttt
     7141 cctccggctc ggcggggcgg ctttccaaac aaggcgcggg tacgctgcgg ctggagggcg
     7201 cgaatgtgga cctgtgcggc gtgcagctcg gtgtggtggg cgcgagtccg gtggtgggcg
     7261 gcacgctggt catcacgaac aaggacgcgc tcggcacgcg ggacacggac acggtggccg
     7321 ccggcgcgca gtatttccag gcgcagttga actccggcac gctgcaaatc gccggcacat
     7381 tgaccggtgg caatgcggtg ccgaacggca tcagcatcgg cgcgcgcgcg gcctcgcccc
     7441 tggtcttcac gggcggggat gtggagtttc tcggggccag ttccctcttt ggcgcgacag
     7501 gcacgggcga tgacatcgtg gtggaggtga acaatcatgt gacgctgccg ggggccttca
     7561 cccagggggg cggcggcgct atcacgggcc tggccctcag cggcacgggc acgctgactt
     7621 ttgccaacgc ggccacgctt ctcacctcgc cgctcaaact tggcggcacg gtcacggtgg
     7681 agctggacac ggccacgctc ggtgcgacga ccgcggcggc tcccgtccac tcgctggcgg
     7741 cggggacgac gctggtcatc ggcaaaccag gcgagacacg cctggtgact gctttcggcg
     7801 gcctcgacgg cgcggcaggt tccaaaattg ccttcgatct cgacggcctg gcacgcggag
     7861 acgactacga cgcgctcgtg ctggcgaagc cggacggcgc tgctggcgct gtggccttcg
     7921 cgggcacgat cgaggtggag ctggtgggag ccttcgagcc ggaaatcggc gacacctttg
     7981 acctgctgga ctgggacgcg agcgtgacgc cggacttcac cggcatcgcc tttgatctgc
     8041 cgccgctgcc catgggccgg gaatggaaca cgaacagctt cgccgccaat ggcagcatct
     8101 ccgtgcaggc ggagagcaat gtggacttca ccatcaccac gcagccgcag tcgcagacaa
     8161 agctcgtcgg gcagccggtg acgttcacgg tgatcatcac cggcaccggg cccttcagct
     8221 accagtggcg gaaaggcgct gtgaaccagg gcggtccgac gagcgacaac ttcctcacga
     8281 ttggcgctgt gtctccggct gacgcgggca cttactttgt ggacatcacc aacgccagcg
     8341 gcacgctcgg cagcgcgggc gcggtgctca cggtcatcac gccggtgcag atcacccagc
     8401 agccggcgcc ggccacccag acggtcatcg agggcgccac cgtgcagatc accgcggcgg
     8461 ccacgggcac cggcccgctg agctacaact ggcgcaaagg caccgactcc ctcggcgcgc
     8521 cgaatgaccc cgtcctcacg ctcaacaacg tgcagatcgc cgactcggga gactacaacg
     8581 tcatcgtcac cggaccgggc atggacaact tcgaggtcag ccaaaacgcc tccctcaccg
     8641 tgaacccggc tccaccgccg gggccgatct cgatcacggg gaacatgact tactcggaga
     8701 attttgacgg gatggggacg acgacaccgg gcaccgaatt ccccaatgga tggaagggat
     8761 ttaaatttgc tgggacgggc gggatggcgc ctggcacgga gctcacaaac ctgaccacgc
     8821 ccgccttcac cgccagcaac gggggcggca cggcgggcac tctttacaat tacggggtcg
     8881 agggcgcgaa cccgcttgca gaccgcgcgc tcggctcgca ggccagcggc ggctttatcg
     8941 gggcttttgg agtgtccttc atcaatgaca ccggcggccc cttggaaggg gtcaacatca
     9001 agatcggctt ccgcagcgag ctttggcgca ctggcaacaa tgcgggcgag gagcgctgga
     9061 tcttcgagtg gaaacttggc ggcggcatca ccgatctcga tctcggcgaa tggaacacgg
     9121 ccagcgcctt cgacattgtc gaaattgacc cgacgaacac gaccaattcg tcgcgcgacg
     9181 gcaatgccgg cggcaacttt gcaacgcttt cccccgcgtt tttttcctcg ctgagcggct
     9241 gggagcccgg gcaggtgctg cacatccgct ggagggatgt ggacaacacc ggcaatgacg
     9301 ccggcatggc gattgatgac ttcaccttca ccgccgaaaa cgtgccgccg ccgccaacgc
     9361 tgctttattg ggacgcgaac ggcgctctgg ctggcgctgg cggatccgcg ccgactggaa
     9421 cctggggcgt ggacactttc tggggcggca ttgcggatgg cacgggtgcc acggcagcct
     9481 ggcaggcggg tggcgatgcc attttctccg ccggggcgga tgccaccggc atgttcaccg
     9541 tcacggtggc cggcacgcag gatctcggcg cgctggtatt cgaggaaggg caggtgacgc
     9601 tctccggcgg aacgctgaat ttcaccgaca tcactccaaa ggtggaagtg ctggacaccg
     9661 gctccgcctc gatcagctcc ctgctgaccg gggcgaccgg cctgctcaag cgcggcggcg
     9721 gcatcctgag cctgaccaac ccggcgaaca gcttcacagg aaatgtggtg ctgggaggcg
     9781 gcatcctggc ggtcacggat gacgcccagc ttggcaatgc cgacaacgac atcgtgctca
     9841 gcggtggctc gctgctgaca tcggtgagcc tctcgctcaa tgcgcagcgc tccatctccg
     9901 gctccggcgg cctggctcct gacgcgggca ccacgctcac gctgcccggc ccggtggcca
     9961 cctcggcgct ttcagtgacg ggcaccggca cgctggactt cacgggcgca agtccgacgc
    10021 ttggctcgct ttccttccag ggtgccgcgg caagcaccgg cgtgcagctt gccctttcct
    10081 ccattggcgc gaaccactcg agcggcacga gcatcgtttc caacgacttg aattttggca
    10141 ccacgaacgc cactgccgag gtggccacgg gcggcacact tcagctcgac ggccagctct
    10201 ccctcggcgg aggcggcagc aacaggctca tcaaaaccgg cggcggcatt ttgatcatca
    10261 gcggcgcgca gcccggcctc aacaaattgg gcatcggcat tcagcaggca tcacccattg
    10321 agggcggggt ggtggtcatc aatgacaagg agggggcggg agccacgcag tccttcttta
    10381 attacggcac cctccaggcg gcggtgcctt tgactggcga cgaggccatc gccatcggcc
    10441 tgtccatcgg aggacgcgac gccagcccgt ccgtgctggc tggcgaggag atgactttcc
    10501 ttggcgacag cacgcttttc gccgccaccg gcaccatggg tgacattcgc ctgaacgtga
    10561 acaaccacac caccctgtcc ggcttgctca cgggatcgct caatgccggc ctgagcggat
    10621 ttgccatcgg cggcaatggc atgctcacac tgaacaatgt gctgagcaat ctcaccaccg
    10681 ggatcaaatt gaaggacacg gtgactgtcg agctggcgcc tgcgagttcc ctgggcacgg
    10741 cgggaatgac aagcgcgccc atggtcggaa ttgatgccgg cgccacgctc gcagtcggcc
    10801 tcatcggcgc cgtccaagaa gtcatcgcct acgcgggctt gcagggatcg gcggggtcgg
    10861 tgctgcgttt tgacatcggc ggcgcggtgc gcggcacggc ctatgacgcg ctgcgcttgt
    10921 tgaagctcga caacgaccag gcgggcgcgg tgattttcgc ggggcaggtc aaggcagacc
    10981 tcatcagcgg attcgctccc gcgctggggc agagttttga cctgctggac tgggatgcgg
    11041 gcgtgacgcc ggatttcacg ggcatctcct ttgatctgcc gccgcttggc gcgggcctgg
    11101 gatggaacac gtcctacttc gccgtggacg gaacgattcg agtcgtcact gatgtgctga
    11161 ccattcttga agagccggac tctgccagtg tcagtccggg ccaggcggtg agtttttcgg
    11221 tggtcgccag cggtcccggc atcatcagct accaatggca gaagctggtg gggatggatt
    11281 ttgagaatgt cgtcaccaac ggcaatgagg ccaccctgct gctgctgaac cccaccacgg
    11341 atgacgccgg cgtttaccgc tgcctcgtca gcaatggcgg cgcgcccatc gaatccgctc
    11401 aagcgaccct cattgtcttc ccgccgctga cggatgtgat ggccacgcgc accccggcgg
    11461 cgaactccta cttcatcatt ggcgacagcg tcacctttaa cgtgaccttc aacaatcccg
    11521 gcggcggcac ggtgacctac cagtggtaca ggggcagcag ccccatcggc ggagccaccg
    11581 gaccttcttt tcaaatcaac cctctctccc ttgccagcac cggcacttac agcgtgcgtg
    11641 tcagcacggg cggaccgccc atttccagta acggcgtgcc cattgtcgtc gtcagccccg
    11701 agcctgccat tgccacccag cccgcgccgc gcacgctcgt cgcggtcggc cagccgctga
    11761 cgcttttcgt cgagggcaca ggccgcccgc cgctgcgtta ccaatggaag cgggacggca
    11821 aggtgatccc cggcgccacc aaggcgagcc ttgtgctgcc caaggcggcg cttgtccacg
    11881 ccggcaccta catctgcgag gtgaccaacg ccgccggacc gctcgtcacc agctccaccg
    11941 cccaagttgg cgtggtgacg gtgaacgcca gcccgccgaa gctgaacctg cctgaaaaag
    12001 gcaaatccgt gatgaaagtg ctcgctgccg ggcctaacct gctctttaac tggacgcgca
    12061 acggcggagc ggtcccggcc aatttcattt cgacacccga cggcaagacg ctcaacggct
    12121 ccaacctcac catcaacgac cacggcagct acgcctgcac cgtcaccctc ggcgtgctca
    12181 ccctgcaggg cggccagcat ttgctccagg tctatgacag cgagccggat tcgccggcca
    12241 ccaccacgat gccggatggc atcatcggcg ggaactacat cttcgacatg ccctacacgg
    12301 tgggcgaccc gccgaccgag gctccggaca gctttagcgc caagggtctg cccgccggcc
    12361 tgaagatgga ccccaaaact ggacgcatca gcggccgccc caccaaggct gtgaccagtg
    12421 tgcctgtgga aatgacggcc aagaacaaac tcggctccac caagaccacc agcactctga
    12481 ccattcacgc cttcccggcg aaactggcgg gtccgttcac cgccctggtg gagcgcgacc
    12541 cggatcttta tggcaacctc ggcggccggc tggacatgac catcaccacg ctcggagctt
    12601 actcgggcag cctcctcatg ggcacgacga agtttgccat caaaggcgtg ctggaccttg
    12661 acgcgctcga ccccaacgga ctcgacccgg acgagggcgt gccgcccggt tttgagatcg
    12721 tcatcccgca aaagaacagt cccgcgccgc tgatcctcga actcgacatg ccgctgctcg
    12781 acgaggacgg cgaccccgac atcaacggcg agcgttttgt cacaggcacc gtgaccgacg
    12841 gcaccggcac tgcggacatt gacggctggt ggcagaagtg gaatccgaaa gccggcgccg
    12901 ccgcctcgga tttccaaggc tattacacct tcggcctcgc cattccggaa aatgacacgg
    12961 ctcttggcga cgcctccgtc ccgcagggcg cgggctatgc ctccttcaca ctggccgccg
    13021 atggcaaata caagctcgct ggaaaaaccc ctgacggcga gtccctcacc acggcgggca
    13081 tctccggacc agccgggcaa atcctcgtct tccagacctt ctacaaaccc gccgccggct
    13141 ccctgctcgg cgtgcccgtc atcagcacca ccggcggcgc cacaccgcag gaccggcctt
    13201 tgatggggga tgccgactgg ctgcggcccg agaacaccaa tctcaagcat cgggtgttca
    13261 gagccggctt cgcattgcag cccatgagca tcatgggcgg gcgctactca ttgcctgatg
    13321 ccggcaacgt ggtcatgaac ctggccccgg acagccaggt cacgatgagc ttcctcgaag
    13381 gcggcatcgg caccacgttt gacgggccgc ccggccagac cagccgggtg gacatgaccc
    13441 tgcgcattga cgccaagaac aagctcatca ccgtggccgg ccccgcactg cccgacacga
    13501 acaagcccaa gcccatcgcg cccaagacgg gccttttcgg cggctccatc ggcctccagg
    13561 acggcaaccc ggtgcccaac ggcaagccca accccgtcaa gcgcaccgcc gcttatcaag
    13621 gcatcctcat ccgtgacggg tcggaataca tcggcgtggg ttatttcctt ctgcccaacc
    13681 ttccgaccga tgccgtcaat ccgcccacca cgcccaccac atcccccatc gagtccggca
    13741 tggtgctcat cgagccgtaa agcagatcaa tttggatcca actcgaaacg gcatcgttcc
    13801 aacgcgacgc cgttttattt gtttccgcca gcccgagcct tctcgccatc cgtgccgcca
    13861 agaccgcgcc agcggaaacg ctccagccac gggtttggcc ttccctccac cgcgtcggcg
    13921 atgagggggc cgagcaccgg ggtgaatttg aaggcatgac ccgaatcccc cgccgccacg
    13981 aaaaggccgg gatgcccggg atcatggtcc aggaggaaat gcccgtcgaa actgtccccg
    14041 tacaaacaga cgcgcgaggc agcaacaggc gcgtccgcca gcgcgggaaa ggtggcgcgt
    14101 agaaaggcgc ggaagcgctc ctcctcgcca tccggcatca ttctcgcgtc cgaggcgcgc
    14161 acacgccggc ccgggccatg gttggccacc ttgaccacac cgttcgcatt ggcggggaag
    14221 ccataccagc ccgtcgtgcc gatgtccgcc gcccaaacgg ggaaagccgg ggcgcgccat
    14281 tccgcggcat tctgcgggag gaaatgaaac acgctttgcg ccgtggcatg gagccggtcc
    14341 gccagccagg gcaggtgctc cggcgtccat gctcccagcg cggacaccac cacatccgcg
    14401 cgccattccg cgccatgcgc gtccatcacg ccgcagacgc gcccgccctc ctcgagccag
    14461 cggtggaagg ggcggtgctc gtggatttcc gctccagcgg ccagcgcgtc cagtttcagc
    14521 cgcgccacca cccgcccgct ctccgcccag ccgccctgcg ggttgaagta gccgtcagcg
    14581 aaggcgccgg cattccactt tggatgcgcg cggcgcagtg tttccgcgcc cactctccgc
    14641 agcggatgcc cgcgcgcggt cagaaaggca tggctgtcat gctcgaacga gcccggcgtc
    14701 atcggccggc gcgtcatgac cagaaacccg tcctcatgaa acaagtcctc tccccagcgc
    14761 gcgttccact cgcgccagcg ccggatcgcg gcctctccca tcgccgtgta aagcgggtcc
    14821 gcgccataat ccatgcgcac cactttgctg atgtccgtcg aggccgcgtc cgggtgcgga
    14881 atgcctcccg cgtcgagcaa aacgacgcgc cagcctcgtg tccgcagttc cagcgccgcg
    14941 gccaggccga agacaccgcc gccaaggatg agcgcctgct tttccatgcc ggcaatcaac
    15001 acggccacga tccgctcaca acgtctcttt ggccgggcgc gcgcggaaaa aaccgcaaag
    15061 gcggcgcgga aacgcaacac gcgcttgcgc gcccccgcag cggccctatg ctcgcggccc
    15121 tcttttagca atggaacaag cccttcccgg acaacgctgg gtcagcgaca gcgagccaga
    15181 gctcggcctt ggcgtggtga tgaaggccga attcggccgg gtggaaatct acttccccgc
    15241 cgccaacgag caccgccagt acgccatcaa aaccgcgccc ctgcgccgcg tgcgcttcca
    15301 ggagggggac acgatcaaga cccacgaagg ccagtccctc gaagtcgagt ccgtcgagga
    15361 gcgcaaaggc atgcttttct atcttggcgc gagccgccag gtcagcgagg ccgagctttc
    15421 cgacaccatc agcttcagca aacccgagga ccgcctcctg gccggccagg tggacgagcc
    15481 gcgcgagttc gatctgcgcg tcgaggctct caagcgccgc gccgccctgc gccgttccac
    15541 cgtgcgcggc ttctgcggcg ggcgtgtgga cctgctgccg caccagatgt acatcgcctc
    15601 cgaggtttcc agccgcctcg tcccgcgcgt cctgctcgcc gacgaggtcg gcctgggcaa
    15661 gacgatcgag gcctccctca tcctgcaccg gcttcatctc accggccgcg cggagcgcgt
    15721 gctcatcctc gtgcccgagc cgctcgtgaa ccagtggttc gtcgagctct tccgccgctt
    15781 ccacctgctc ttcgccatct ttgacgagat gcgctgcgac tccatcgaga ccaacgagga
    15841 aaacggcaac cccttcctcg acagccagct cgtgctctgc agcaccgcct tcctcgccgg
    15901 ctcgccggag cgcgcgcgcc aggccgccga ggcgggctgg gacctcctca tcgtggacga
    15961 ggcgcaccac ctggagtgga ctccggagcg cgccagcccc gcttacaccc tggtgcaaag
    16021 cctggccgaa aaaacccccg gcctcctgct cctcaccgcc actccgcagc agcttggacc
    16081 cgaggggcac ttcgcccgcc tgcgcctcct ggaccccagc cgctacgccg atctcggcca
    16141 gttcctcgag gaaaccctcc actacgagga agtggcccgc gcgctcgacc gccttctggc
    16201 cggaaaatct ccaaccgccg ccgactcggc gctcttcatc aaaaagtcac cccacctcca
    16261 ggagcatctc gacgccatga aggccggcac cgagggcgcc cgggaaaagc ttgtctcatc
    16321 ccttctcgac gagttcggca ccggccgggt catgtttcga aacagccgcg ccgccctcag
    16381 cggcttcccc cagcgctccg ccaaaatcat cccattggag gctgcggacg atccgctcga
    16441 cgccaaggtc aaatggctgg ccggactgct caagaagctg ggcggtgaca aggtcctcct
    16501 catttgccgc agccgcgacc tggccgtcga gatccacgaa aaactgctcc atgaggtcaa
    16561 cctcaacgcc gccgtcttcc acgaggggct caccctgctc cagcgcgacc gccaggccgc
    16621 atggttcgcc gaggaggagg gagcgcgcgt tttgatctcc tccgagatcg gcagcgaggg
    16681 gcgcaatttc cagttcgccc atcacctcgt tctttttgat ctgccggacg atcccgaact
    16741 gctcgagcag cgcatcggcc gcctggaccg catcggccag agcagcacca tccacatcca
    16801 tgcgccctgc atccagggcg gcccggagga agtgctggcg cgctggtatc acgagggcct
    16861 gcatgccttg gaggccaatc tccacggcgc caccgagatc ctccacgccc tgcgcccgga
    16921 gctggacgcg ctcctggccg cgcccgaacc ggaccgcctg gaggccttca tccagcaatc
    16981 cggcgagctg cgcaaaaccg tcgcaaaaaa actcgagcgc ggccatgacc gcctgctcga
    17041 actgaactcc tgcaagcctg aaaaagccgc ccaggtcatt gacgacatcc gcgcccagga
    17101 ccgtgacacc gagttcgagg agttcttcat ccggctcgtg gaccacttcg gcatgcatgt
    17161 cgaggagctg ggcaaccgct cctacttcct cctgcccggc aacctcacca ccgacgcctt
    17221 tcccgcgctc cccgacgacg gcctgaccgt caccttcgac cgcgcgcgcg ccctttcccg
    17281 cgagaacacc ggcttcctga cccccgacca ccccatggtg cgcggcgcgc ttgatctcct
    17341 gctcggcgcc gagggcggca acagcagctt cggactctgg cgcggctccg gttccgaggg
    17401 cctcatgctc gagacgcatt tcgtcgtcga gtgcatcgcc ccgccggagc tgcatgtgga
    17461 ccgtttcctt tcacccggcc cgctgcgcgt ggtcgtggac catgcgaaga aggaccggcg
    17521 cgatgacgcc gccttcatgc atgccaggat cgagaaaggc agcccctcgc gcctttttga
    17581 aaatcccagc atcaagcgca aaatcttccc cgccatgctg tccaagtcgc gcaagatcgc
    17641 caaggcggaa atggaagcgc tcgtcgaggc ggccgtgtcg tccatgcgcg cgcagctcca
    17701 ggccgagatt gagcgactcg aagatctagc ccagatcaac gaccacgttc gtccggagga
    17761 aatcgaggcc atgagaagcc acatggccct tctcgaaaaa gcgctcggct ccgcccggct
    17821 ccgccttgat gccctgcgcc tcgtcctgcg gatgagctga ggcggcgctg ccgcgcgcga
    17881 gcagtcttgc cgcggagccc cgcgatcgaa aaaaagcgcg aagcccggcg tgcggacttc
    17941 gcgcattggg attcaacttg cgggatcaca ccgcccagcc gccgcggtag tcgcgggtga
    18001 gccatttggg atcaccgccc tcggcgaagg cgcgctcgac ggggttccat ttgaaattgg
    18061 cgccgtgcca gtaggccatg ttcatcaggt ggcaggagat cacggtggtg gctccgatgg
    18121 ccacatcgca gatcggcggt ttgcgggtct ggatgctgtt gagccagtcg tcgtggtggt
    18181 ttttgctttc gtagagcctc accttggcat ccttcaaata ctcgcgctcg ctgagcacca
    18241 cctcgcggtc cagggaggtg cccttgtcct gctcccggtc ataaaagccg tgcaccttcc
    18301 tgccgccgag ccagagttcg aacttgccgc ggttcacatg cacctcgccc tcggtgccgt
    18361 agaaggagac gcccttgccc tctccgtggg tgagcacgac tccgttcgca taaacgagct
    18421 gcgcgccgcg cttggcatct gcgccgccct gcggtgcgcg cacttcgacc gggccgctgt
    18481 cgtccacgcc gaggccccac tgggcgatgt caatgtgatg cgcgccccag tcggtgatca
    18541 tgccgccgcc gaactcgcgc gtcatgcgcc aggcggggaa gtggttgtgg actccgcgcg
    18601 ggctgaggat gctgttgtag ggcttgagcg gaccggggcc gcaccacatg tcccagtcca
    18661 ggccgggctc ggcgggttcc tcgcccaggt tgtaggggcc ggcggggtcg ccaaaggagg
    18721 tggtgatggt tttgatctgg ccgatgacac cattgcgcac cagttcgcag gcgacgcgga
    18781 attccttgct ggagcgttgc tgggagccgg tctggaagat gcggcccgag tcgcgcacgg
    18841 ccttggtgag caagagcgcc tcgtggacat tgtgggtgag cggcttctcg ccatacacgt
    18901 ccttccccgc cttcactgcg gcgatgccga tgtaggcgtg ccagtggtcg ggcgtggcga
    18961 cgacgacggc gtcaatatcc ttgcgcgcca gcagctcgcg gaagtcgctg taggcggcgc
    19021 agcctttgta agcgccatca gcctttttgc cgtaagtttc atccacgcgg cccttcgcat
    19081 gctcgcggcg ggtggtgtcc acatcacaga cggccacgac ctggcagttt tcacggccga
    19141 ggaagttgcc gaggtggccg ctgttcatct tgcccatgcc aatgaagccg agattgacgc
    19201 gggagttggg cgctgtttcc gccgaccaca cgcgggaagg caggatgaac ggcgcgctga
    19261 cggcgccggc ggcggttttg aggaaggaac gacggttgtt ttggctcatg ggatgaaaag
    19321 gtggatgcac aaaacgcggc ggcgaaagaa attgtgcagc cgtcccggaa aaagatgcgc
    19381 gcgcggcggc gacaaaaaaa tacccgcgcc acggcggggt ggcgcgggtt gagggatggg
    19441 agggccttgg cagccggatc aggaaatgac cggcagttcg aagccgctgg cgtattcctc
    19501 catggcgatc ttgttcgcct cgtcggcgaa ctcgcccacg aacttctcgc tctcagggtc
    19561 gaattcgagc caggggccga cagcgggcag gtctttggag agatccacct ggttggcttc
    19621 gaggttggcg aagaagtcgg agagagtttc ctgcgcgggc ttgctggcgc tgaggcgctc
    19681 tttgacttcg ccggtgccca gtttcttgcc aaggcggtag gagacgttgg ccaggtgggc
    19741 cagggcggcg gcgtggtggc cgtgggccac atgcagcacg tccttggtga tcttgccggc
    19801 gcggacggac tcgatgaagt tcagcatgtg gtcggggccg tcctggaagt tgtcgaactt
    19861 ctcgatggct ttgccgtcgt tgtcataggc cttggactcg gccacgaaac cgccctcgca
    19921 gtggatgacg ttgccgatgc gcggggcgga ggtcttgccg ttgatgcgga agacgggctc
    19981 gaagcctttc gtccagttca tgtccttcat gggcaggccg cggttgtcaa agagcagcgg
    20041 ggcgttgccg gggtagtcgt agtaggccat ctggttgttg gcggtctggc cgtcgtcatc
    20101 atagccccag cgtccgccga agctcatgag gcgcacgggc agcttgtcgg ggttgccgag
    20161 cgcccagcgg gccacgtcga gctggtgcgg cccctggttg ccgatgtcgc cgttgccgta
    20221 ggcccactgc cagtgccagt catagtggaa tttggcgcgg ttcacgggca gctcggtgcg
    20281 cggcgcggac cagagcttgt agtccacggt ggcgggcggg gccaccgggg cgtccacttt
    20341 gccgatgctc tggcgggctt tgaagttgat gccgcggctc agggtcactt tgccgatgtg
    20401 gccggccttg acccactcca tggcggcggc ccagccgagg tcggagcggc gctgcatacc
    20461 gtgctgggtc accaagccct tcctggcggc gatggcggcg gcttcgacga gcttgcggcc
    20521 ttcccagatg ttgtgggaga caggtttttc gacgtacaca tgtttgccat tggccaggcc
    20581 ggtcagcgcg atcagggtgt ggctgtggtt cggcgtggcg atggtgatgg cgtcaatgtc
    20641 cgggtcggcg cagcattcgc ggaaatcctt gtactgcttg accttgatgt ccttcttcgc
    20701 cagttcggcg acttttttgt ccatcacttc gctgtccacg tcgcagaggg ccgcgaggcg
    20761 gacgccgggg attttcagca ggctgctgat gtggccgttg ccgcgggact tgaagccgat
    20821 gacggcgacg cgcacgtcgc cgttggctcc gatgggtttg gcccaggcgg gcagggtggc
    20881 gagaccggcg gcgccggcga gggtgccgcg catgaaggcg cggcggctgg tgtttgtcgg
    20941 gttcataagg aaacgaggtt tgggttcaga ggcgcggatc atctccgcac gtcgcggaaa
    21001 atggcaggct aacaaaaacg ccgcgcttca ggcaaccttt cttttcgccc gatgctggaa
    21061 agtggtgctt ttgtgcctcc cggcagtctt ttaccgctgc ttgacgccgt ctcattcacc
    21121 gtcaccgccg aaccacaggc tcatggagcg gccggagtag ccggtcatct ccaggtagcc
    21181 gcggccgcgc acggctttgc ccgcgcgggt gccccggtaa tccaccgcgc cctcccagta
    21241 gccgaggccg ggggtggcgg cggcttggaa ttcctgctgg ggcagcacgg cataggactc
    21301 caggcgcagg ccgaggcgcg gcacttccag gctccagccg gtggggtagc gcgctccggc
    21361 gggggagcgc cacatctggc cgggggtgag gcgcagatcg tccgcggcca gggcctcggc
    21421 gctgccgtcg ggatggatga atgtgcccgc gctgccggtg gtggcgccgc cgttttcgcc
    21481 gcgcagttgg aagagcatca gctcgctgcc atcgtcgagc tgcgcgctga accagtccca
    21541 cccggtggtg cctttttcca ggaagctgct gccgaattcg tggtccatcc agctcaggcc
    21601 ggccacctca tggcgcgcgc cgtccatcgt caccgtgccg cgcgtgggca ggcgggtgaa
    21661 ggagtaataa tggctggcat tgccgggcgt ggggcctttt tggctgatgc catccctgcc
    21721 gtggaagacg ggcggcttgg ccttttccag ctccagctcc aggccaaacg cgccctccgc
    21781 cgcgcgcagc ctcatgccgc cgggctcgaa aaggcactgc cagtcctcat tccacacgcg
    21841 ctcctgcgtg gacccggcca ggccggggcc ggtgcggttc aggcggtcgg cgtggtggta
    21901 ggcgcggccc tgggcatccg tcacggccag gtgggccatc cacacatccc gcagcgccca
    21961 gggggactgc acggcgggct gggcgacggc gccgatgcgg aaaaaggtga gctggtagcc
    22021 aaagcgccgc ccctcccgcg tgtcgaggtt cccggtgaaa taccaccact cgatcttgtg
    22081 ccgcgggtgc tgggcgtggt cgcgcgggaa ttcgtagcgg taaccgggct gggcgggctg
    22141 ccagggtggg gcggcaggtg gcgcggccgg gccacccccc gcctgctcaa agccactctc
    22201 gggagccggt gcagcctccg ggggcgattc ctggaggttt tggcgcggct cagaacccgt
    22261 accaaacagt ttctgatgcg taccaaacgg tttctgacac gtcacaaagg ctttttgacc
    22321 cgtaccaaac ggtttctgac acgtcacaaa ggctttttga cccgtaccaa acggtttttg
    22381 acacgccaca aaggcttttt gacctgtacc aaacggtttc tgacacgtct caaaggcttt
    22441 ttgacctgta ccaaacggtt tctgacacgt ctcaaacggc tctttagccg tcacaaacga
    22501 cgttggcttt gtcccaagtg gctcccaaac ggcacgattt gacatcggcg cgggcgtgct
    22561 ggcggcctgt gcatttcctg agagcaaaag caccgccatg ccgcccaggg ccagcttttt
    22621 caccgcgcgg cgggcgggcc agaggccggc gagcatggtg cagagcacgg tcagcacggt
    22681 ggagccgagc agaaaggccc agggggtgtg aaattggatg ctccagccga agctctgcgg
    22741 attgatgaca tggatgagca ccagggagag cagcgcgccg accacgagcc cgagcgcctg
    22801 gctgacggcg ccgatgaggc cggactcgat caaaatggtg cgccgcacct gcccggcctc
    22861 cgcgcccgcc aggcgcagca gccgcagctc ctcccgccgc tccagcacga gcgtgagcat
    22921 ggtggcggcc acgcccgcca tggccacgag cacggcgatg atctccagcg cccaggtgat
    22981 ggcgaaggtg ccgtcaaaga tgcgcagcac ctcggcgcgc aggccggcat tggtgaacac
    23041 ggagacaaag ccgcgcccgt ccagggaggc gagcatttcc tcccgcacgg ccccggcgtc
    23101 ggagcccggc ttcaagtaca gggccaggtg cgtggggcgg ctggcgccga agtgtttctc
    23161 aaacagggcc tggtccaggg tcagggtgcc gccatcgctg gagtaatcat aaaactgccc
    23221 ggccacggtg aaggtgcgcg gcccctgcgg cgtggccagc tccacgctgc cgccctcccg
    23281 cacgccgaag cgcagggcga agctctcgga gatgaagacc tgcccctttt cccgcgcgcg
    23341 ctggagcagc gccttcgggt cgccgggcgt cttgaagacc atgcgcccgt gctccacctg
    23401 cacggcgaag tcgcccgtgc cgagcttcac gatgcgcccc tggtagggca ggtccagggc
    23461 gcggtagcca tccgccgcgg ccacggcggg gtggccggtc agcaaatcca gcgtctgctg
    23521 ggaaagcgtg ggcgcgccct ggctgcgcgc gggggtgccg gggcgcacat acaggtccgc
    23581 gcccatgctt tgatccaccc acagcagcac cgtctggcgg aagctgccca ccatgacggc
    23641 gatggccacc gtcagcgcca ggctcaccgc cagcgccgcc acggagacgg agagccgccg
    23701 cgtggacgcg cggatct
//
LOCUS       DAOMYJ010000061        23680 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Prosthecobacter sp. isolate AMR_MDS_5629
            NODE_769_length_23680_cov_21.371302, whole genome shotgun sequence.
ACCESSION   DAOMYJ010000061 DAOMYJ010000000
VERSION     DAOMYJ010000061.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867850
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prosthecobacter sp. (wastewater metagenome)
  ORGANISM  Prosthecobacter sp.
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Verrucomicrobiaceae; Prosthecobacter.
REFERENCE   1  (bases 1 to 23680)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 23680)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.30
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:34:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,742
            CDSs (total)                      :: 4,684
            Genes (coding)                    :: 4,676
            CDSs (with protein)               :: 4,676
            Genes (RNA)                       :: 58
            tRNAs                             :: 55
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23680
                     /organism="Prosthecobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_769_length_23680_cov_21.371302"
                     /isolate="AMR_MDS_5629"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1965333"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..554)
                     /locus_tag="PLP58_08470"
     CDS             complement(<1..554)
                     /locus_tag="PLP58_08470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ08587.1"
                     /translation="MTALRLIILLLCLGGGGWHLERERQAGRLRQVDEWFLDFLTANA
                     RERLETPDPAAAPEVALVTLRERDRAEYAAWPPPPLDWRTILQGLAPFEPDVLVVAAP
                     LNWGQPAPDFLPALAEALLAFPSVVLGMEAQWQDVPPPHAPFLGGLEDQLPRLSNVSG
                     DAELAPSLKALVTAPDPALLGQSEL"
     gene            complement(606..1091)
                     /locus_tag="PLP58_08475"
     CDS             complement(606..1091)
                     /locus_tag="PLP58_08475"
                     /inference="COORDINATES: protein motif:HMM:NF020123.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HRJ08588.1"
                     /translation="MKTARILLALALTALASTTLLATAGKDNVGTVITDLKVRFSGAS
                     PDLKGKAYVLEFWATWCPPCRTSIPHLNELYKKHKDKGLQIIGVTKEDRQTVRNFEKN
                     IPIEYTVAHDTYGKLSEDFQIKGIPHAMIVDKTGKIVWEGHPMSLPESQLEAVLKAGV
                     E"
     gene            complement(1162..2070)
                     /locus_tag="PLP58_08480"
     CDS             complement(1162..2070)
                     /locus_tag="PLP58_08480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009958226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ08589.1"
                     /translation="MMKPTLFFLLALGVSSLSAREYRDLTNLDGVTIKAELLDLTDDG
                     MLKVNVNLKPFQIPLASLSQADQDWLRLWDLERKQGKEAAYYSRVIFEDDFSGGAFKE
                     GWGHYKSGSVIQDGVLVGITPEGSDHSAVDNIKFPGEQDLQVEVKFRFVSEAAKSFNV
                     WFDDKDYKGAHAGHICQVTVSPAQVQMSDAKTGGFTLENGLYDRKKANQLTDEEKKML
                     ESKTKRVPVKLELQKWHTLTARTKGDTIFVLIDGKEAGEFSSEGVAHATKSLVSLTTN
                     AVDVHYDDFSVKAAAKPLSAARPAAR"
     gene            2315..3439
                     /locus_tag="PLP58_08485"
     CDS             2315..3439
                     /locus_tag="PLP58_08485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ08590.1"
                     /translation="MMNRRALLASLAAATVCRGADERRFFTVAQRKGRWWFITPEGRP
                     FFSIGLNHIDPAPLRYASNGDLWERKYGNSMEKWLKEAVAPDLKAWGFNSVGWTQEVV
                     TRGSTNHRHSRAFTFEEYQWLGLPYCHQLPFADFHQWEAETRHPDFDAPEFAEWCDYV
                     AREHCARLKDDPKLIGYFYIDCPTWVHTRPESAWKGPLYDPEKLKTEAGRKELHELAT
                     KWYRITHDAIRRYDKHHLILGDRYEAANPIADEVIDAALPYVDVLSFQHFSGPDKVTA
                     NLNHWHQKTGKPVLLADHAIQIREADGSGRHDGEGDARVLAALRETPGCIGYHLCGAY
                     LRNEARKRALRDAAEKPDEAALEAITRANRETAAWMERVR"
     gene            complement(3443..3868)
                     /locus_tag="PLP58_08490"
     CDS             complement(3443..3868)
                     /locus_tag="PLP58_08490"
                     /inference="COORDINATES: protein motif:HMM:NF024861.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain-containing protein"
                     /protein_id="HRJ08591.1"
                     /translation="MRVMLDANVLLDCLVLETSGQARPGKDGSERLLNLCDSGVHQGL
                     VAWHTLPIIAYYHAKQNASEATSAMMDELLLMLEVPTVGQPDATDWRSHGIHDFEDAL
                     QMACALSGRADVFITRNTIDFVGVAMPVMTPEEFLAAFP"
     gene            complement(3875..4123)
                     /locus_tag="PLP58_08495"
     CDS             complement(3875..4123)
                     /locus_tag="PLP58_08495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ08592.1"
                     /translation="MKTLTQSTPLGTLGLPSGDHWVKFHVEGDSISFEVAASVPAKAN
                     SPAKKKPAGFVQKWSGTAKKIEDAGDTWLAHINEKHLR"
     gene            complement(4169..5197)
                     /locus_tag="PLP58_08500"
     CDS             complement(4169..5197)
                     /locus_tag="PLP58_08500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009960537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HRJ08593.1"
                     /translation="MKSAAVVNFAPEKGSVEIREIEKPTIGAEDVLLEVANVGVCGSD
                     LHQWTSDHSWPVNYPVVLGHEFGGHIIEVGKDVEGWKEGDRVVSETAAIISKDNPMTR
                     RGLYNLDNTRKGFGYGVNGAMTKYVRVPSRILHHMPDHVPFEQACLTEPCCVAYNAVV
                     KNARIEPGDRVIVLGPGTIGILCAAMAKQCGAQVAIVGLQQDAHRLELARKHYGCEVI
                     IGDAKPWAMERDGLGCDGVIDAAGASITLKIALELVRPAGWISKVGWGPQPLGFNIDP
                     LVQKNITLQGSFSHNWPIWERVIALMSSGQFDVKPIIGGVWPVTEWHEAFEKMHKGEV
                     VKSVLRPV"
     gene            5327..6613
                     /locus_tag="PLP58_08505"
     CDS             5327..6613
                     /locus_tag="PLP58_08505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009962452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1552 domain-containing protein"
                     /protein_id="HRJ08594.1"
                     /translation="MNIATKRHLDRRAFLRGTGAVLSLPFLEAMLPAFATRAQAAAGT
                     PPPRFLAMCATLGFHTPYLFPKETGADYTLTPYLEPLRDLRSDFSVISGLNHEQQNGA
                     NGHTSEMTWLTAAKHPGLAGFKNTISIDQLIAERIGTQTRFPSLILGTGSESLSWSAS
                     GVPLPAESSPSRAFQQLFVDGTPAEVAAQVRALKRGRSILDTVMGQAKKLRGELGHRD
                     QEKLDEYFSAVRDLEGRLVQNEEWAQRPKPKVDAKPPTDIQSRTDAIGKMRLMQDLVV
                     LALQTDSTRTVTMRLSGMNAVPEIPGVKNDWHNLSHHGQDPAKIEELKVIELAEFTAF
                     ADFLAKLKQTRESGRPLLDSTAVLFGSNLGNASSHSTANLPLVLAGGGFKHGRHLAIE
                     KDKHVFSNLFVSLAQRMGVETDRFGHSTGVLDINQA"
     gene            6674..6931
                     /locus_tag="PLP58_08510"
     CDS             6674..6931
                     /locus_tag="PLP58_08510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ08595.1"
                     /translation="MSIEAAFHRGIVPLLRLLLPGKEDAVLGTTPDKALHERIEWLAA
                     RNTEGELTAEELAEYEGYVRANKFAAVLRREALRMKEETGR"
     gene            7397..9796
                     /locus_tag="PLP58_08515"
     CDS             7397..9796
                     /locus_tag="PLP58_08515"
                     /inference="COORDINATES: protein motif:HMM:NF019259.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1592 domain-containing protein"
                     /protein_id="HRJ08596.1"
                     /translation="MTLRAALRLPSLLCLLLASAHAATPAARPFLDRHCMDCHDAEMK
                     KGGLDLSALSTDGADAAALKKWVRVFDRVAAGEMPPPKKARPAAEETSAFIAALGSDL
                     TAKSEARKGTVLRRLNRQEYQNTLNDLLGVRVSVVDMLPEDGRAHGFDTVGEALSISG
                     IQMQRYMEAAETALNAALMAGEKPELRKTAATLDTGRNKDNIGKHWLKKADGAVVVFN
                     EGGFPSTVIPDLRAHATGTYKVRVTGYGHQVEEPVVFALRTGSFNFRNTDTRTHSFHE
                     LPPDKPGTVEVTLHLPQGHGIQVSPQGLYGPDGHSAVKDGPDKYPGSGLALLSVEIEG
                     PLIEEWPPRGQKLLLGDAKLREVMPEKPWMRGKAGYKPVFTADSADPAADARRLLPAF
                     ASAAFRRPADAATVEPYVRLFDAEFAEGKDFLAAMRTAATAILCAPDFLFLKEPSGAL
                     DRLQLASRLSYFLTRGAPDAELLAADLAQPAVLRAQTERLLQSPALERFVTDFTDGWL
                     NLREIEFTTPDKQLYPEYDELLLDSMLRETRAFITELIRGNLSAANVIHSDFAMINAR
                     LARHYGIPGVSGQHIRKMPLPPDSRRGGVLTQASILKVSANGTNTSPVVRGVFVMDRI
                     LGIEPSPPPPGIPGVEPDIRGATTLRELLDKHRDSETCNGCHRVIDPPGFALESYDVI
                     GGWRERFRSIDQGEKVDLKVTGRKVRYRLGPPVDAAGELPTGAKFADMGGFQKLLLAG
                     QDRVARCLLEKLLTFATGREMGFSDRAELDQLTAASQAKGHGVREMIHAAVQSGIFRR
                     K"
     gene            complement(9818..11230)
                     /locus_tag="PLP58_08520"
     CDS             complement(9818..11230)
                     /locus_tag="PLP58_08520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HRJ08597.1"
                     /translation="MNDSPNRPGTLEKALKLNLDRGIYGTFAEIGAGQETANWFFRAG
                     GASGTVAKTMSAYDMTFSDEIYGKSARYVSRQRMAAMLDHEFQIVQQRLHAKRGADAA
                     FFALANTVRARGWRDTNEECHGWLGLRFQTEPGGPPNDIQLHVRLLDDTNHRQSEVMG
                     ILGVNLLYAAHHYRDELTVFLKSLLDDLRRKRLEIDMVDFIGPAFAKPGFEDRIVALE
                     LVRSDLADAALFGASGEILQASDVLYNKPVVLNRGSFDPITRVNLDMIERGAAAFDQD
                     FGEKALGHVEILEITMHNLLCMDDCAVEYGNFLARADILQALGKNVLVSKYAEFHRLS
                     GYLARHTREPIGIILGLPLFEELFNERWYADLEGGLMEAFGRLFRNQVRLYVYPAGDP
                     LTGKIRTASQARVTRGQDHLLRYLLDTGAVHEIREGLEECLFQTSSNVRQMILGGDRR
                     WRDFVPPIVLERGPWAALAG"
     gene            11377..11467
                     /locus_tag="PLP58_08525"
     tRNA            11377..11467
                     /locus_tag="PLP58_08525"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:11411..11413,aa:Ser,seq:gct)
     gene            11528..13219
                     /locus_tag="PLP58_08530"
     CDS             11528..13219
                     /locus_tag="PLP58_08530"
                     /inference="COORDINATES: protein motif:HMM:NF015722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HRJ08598.1"
                     /translation="MKPRAAWGKTPAVSARILPSRPLLSALAALCAACPAGAQTPNAQ
                     DGHTGSLGMKFVRVPGTTVHFAIHETTVAQWQAFVREGGHAWDYKPHFEQGPDHPAVG
                     VTLQDARAFCGWLTEKEQKEGRLKSEQGYRLPTRAEWDAAAGLLRTRKPDLTLDDKLA
                     DDRAFPWGQEWPPPKGAGNLAEGEIPGYEDGFPFTAPVGQFKPTADGLHDLAGNVWEW
                     CWDPEIRAEQHGVLRGGSWAYFYPESLRSAYLYTVPAALRMPTIGFRCVYEDKARTAT
                     LLAAMKADADKLRADRRQALTGAEVSQDEVAAMRKRFATGGEAANLPDPASLKPAAPG
                     ARHLNSLGMDLVPLGSSALIAAIETRAQDYEAFLKASGRAWTKKPTFLISSAHPAVSV
                     SWDEAAAFCAWLTEKERAAGLIGPGASYRLPDDLEWSRAAGLEDETGADPAERGKSPS
                     AHYPWSATGEFPPPPFSANLDAGRIPGYSDRHSYTAPVTNDPANSLGIHALGGNAAEW
                     CLDAWPGAENERVIRGGSWLSFDKDQLRTGHRQHAARDSATAGTGFRVLLDFNRP"
     gene            13232..14956
                     /locus_tag="PLP58_08535"
     CDS             13232..14956
                     /locus_tag="PLP58_08535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ08599.1"
                     /translation="MPDPSPDDLVLNPRPWFTWMLSALGVLGLAAWFAGPPLAEWGRE
                     KLAEHFISQAETHLDNLEFAACATAIRSARRWSMQNGRVLRVSIEFLDKAGGEDTAKL
                     HMLRELVESGQATLAERARIGRLLLARGEIQGAREELARFTDEEKSAGPVLELRAGIR
                     RAEGFPDEADRLLHQALTLTQADDPDARLRLALMDLRAPYPETRGSARERLFDIAAGG
                     DTRALQAIERLAADSEISGAEALRLLDLVEKHPLKTEQRRLDTLAALLRVRPQERERI
                     MERETRGAADMDEEQLAQLANWLVARGEAARVIELVPLERARKSTKLFHSHIAALAKL
                     ERWDELEAELKRTSGVIPMSRPGIHLWLARAANRHRDGIPQVRQALQAALMAAGEGSD
                     AASSQLALAVAEETAQWDLAIQACEGIAKHHPRTRAAMIEKVLDISLRDKDTPGVVSA
                     AARLAELQPQNDQAVFRARWFSLVAGEGIETAIQALQAESARYLRPGPRHLLLALAAW
                     RMGDLEQTRAHLEHVTDPGELSVGQRAVHAGLLSACGSVGPAFQLAERVPPILLLPEE
                     LRFLRRAL"
     gene            15023..16375
                     /locus_tag="PLP58_08540"
     CDS             15023..16375
                     /locus_tag="PLP58_08540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012419102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005283 - amino acid:sodium symporter
                     activity [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:alanine symporter family protein"
                     /protein_id="HRJ08600.1"
                     /translation="MNFSEFAAAFSGYVWGTPLIVLLFGTHLFLTFRTGLIQRYIPRA
                     IKISFTRSKEGEGDVSQFGALMTALAATIGAGNIIGVAGAVAAGGPGAVFWMWLTGVF
                     GIATKYSEAVLAVKYRVQMPDGSFAGGPMYALERGLGQKWLGVVFAVFTCIAAFGIGN
                     MFQANAVVETLKEWVKPGAEADAVLRWVAGLVMAAITGAVVLGGVRSIARVCGVLVPV
                     MALGYILGCVLILIKNAALLPGALGEIWDGAFAGQAAVGGFLGATIRQAVQAGIARGL
                     FSNESGLGSAPIVAAAAQTRNPVRQALVSSSATFWDTVVVCALTGLVIVSSGHWKEGL
                     PKAALTNAAFEELHTIGPIFLLFGLMTFVFSSILGWCYYGEKAAEYLVGPRAVKPYRI
                     LWVIMVLVGAVQPAAAVMDFSDAANGLMAVPNLICLILLSRVIAAETRAHRHELTGTD
                     "
     gene            16449..17915
                     /locus_tag="PLP58_08545"
     CDS             16449..17915
                     /locus_tag="PLP58_08545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ08601.1"
                     /translation="MHTERALILASLCLAFASLDLAAAAPVLRAGAATSNITPTIAAV
                     PVGRTSRPPATHVHDDLHARCLVLDDGEAKLALVVCDLRHISAEVAAAAKVIIEKTTG
                     IPPQCVVISATHSHTTGGARLEEREGEPYFEYSAFLTRRIADGVLRAHNQLEPAQIAW
                     GVAEELTQVFNRRWFVMPERGPIYGAHDNVEKVDTNPGYKGLLKAAGPVDPQITFLSV
                     RSTSGKPISLMASYGLHYVGGVTPNTISADYFAIFADRVGELLGADRHQDPPFVGIMA
                     NGTSGDVNNLERYSDEELAKRGPQPKKKYQPFEKAREVADLCASKVIEALKTVKWHDH
                     VKLSSVQRTLIFERRYPTKEEVAWAESVKARRIKPMSTSRYSTYSTVLAYASPDMPPN
                     VDVIVQTHRIGDLAVAAMPFEVFAEIGLELKQRSPIQPLMNISIANGSHGYLPTPEQH
                     KLGGYETWIGVNKVQLDASVKMVNALVEMLGELAAEKP"
     gene            18145..18519
                     /locus_tag="PLP58_08550"
     CDS             18145..18519
                     /locus_tag="PLP58_08550"
                     /inference="COORDINATES: protein motif:HMM:NF016430.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor"
                     /protein_id="HRJ08602.1"
                     /translation="MNPPRSPAFPATRWSIVAASQRPDDPRASTALADLCRDYWFPVY
                     AHIRSQGRSQQDAEDLTQSFFQRILEKHTLTHATPERGRFRNFLLASVKHFLINQHEH
                     AKWPKPSASPPQPTSRTNSTPC"
     gene            18709..19191
                     /locus_tag="PLP58_08555"
     CDS             18709..19191
                     /locus_tag="PLP58_08555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ08603.1"
                     /translation="MHIPTLYLDTSVIGGYFDDEWKEATQEMFQRAELGMYQFVASVV
                     TAREIMGAPPEVQAHFASTFTDEAQILELNDEAETLASAYLTAGVVTPKYADDARHIA
                     VATVHALPLVVSWNFKHLANIKREAGFNAVNLLHGYPQIRILTPLQLLNDETDEDQNL
                     "
     gene            19172..19351
                     /locus_tag="PLP58_08560"
     CDS             19172..19351
                     /locus_tag="PLP58_08560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ08604.1"
                     /translation="MKTKTFDAVTEARRWKADVSAKTQTMSREQVLAFFDKDKAMLAM
                     AQMRRGTGEPVKARA"
     gene            20002..20655
                     /gene="plsY"
                     /locus_tag="PLP58_08565"
     CDS             20002..20655
                     /gene="plsY"
                     /locus_tag="PLP58_08565"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR00023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008374 - O-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HRJ08605.1"
                     /translation="MTALLLAALGGYLCGSLPFGYLAGRLRGVDIRKHGSGNIGATNV
                     IRVLGKGIGIPVFILDLLKGWLPVWLAAGWLGTAEGVAGTALQSAGVVLTGFAAVLGH
                     MFTFWLGFKGGKGVATTAGVLLGIHWLAMVGGLGVWLITYFVTRYVSLASMLAGVGVA
                     GTMWVEMSRSGRKDPVMLGFGILVMVLVIVRHRANIRRILDGTEPQAGRKKETSAGE"
     gene            20700..21506
                     /locus_tag="PLP58_08570"
     CDS             20700..21506
                     /locus_tag="PLP58_08570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009963968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ThuA domain-containing protein"
                     /protein_id="HRJ08606.1"
                     /translation="MKFHLPLLSALCIAGLIAFAAEPETPKALRVLMVTGGCCHDYEN
                     QKMILAEGLSSRANVEFTIVHEEGPDGKKDKTHRISIYEKDGWAAGYDVVLHNECFGG
                     ITDNAFVERIARPHHEGVPAVMLHCSAHSYRAAATDEWRKCVGQKSMSHEKNRDLLVK
                     NIAPGHPVMKGFPMEWLDKADELYKNEVLYPDFVPLAKAYGEDTKQDHHLVWVNTYGK
                     GRVFGTTMGHNNSTMEDPVFLDLVARGLLWSCGKLNDDGKPLPGYESRQK"
     gene            complement(21549..21833)
                     /locus_tag="PLP58_08575"
     CDS             complement(21549..21833)
                     /locus_tag="PLP58_08575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ08607.1"
                     /translation="MTQLLYLSRSFVEFGPFTTDELLGFHQRGILQDTDYVRAETSDD
                     WIHVYDWHASLTPATSAAAEKKPAAKKAAAAPAPAAKKAPAKKAAKKKAD"
     gene            21962..22534
                     /locus_tag="PLP58_08580"
     CDS             21962..22534
                     /locus_tag="PLP58_08580"
                     /inference="COORDINATES: protein motif:HMM:NF014119.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HRJ08608.1"
                     /translation="MSEEPHFIHAPSPPGGEPETPPPSGKGGILRRLRDSKRVRLWFA
                     ARHLKLKRDYRPMLLGRPMSREEVGAAGEALAARWLAGHGRRILRRNHRGPFHGEVDI
                     VARHGRVLTFVEVKTRTSAEFGRPADAVDAQKRLLIQRGGQDWLRLLGRPRVRFRFDI
                     VEVLLIPGMKPHVNVIVNAFQMPDSSMSGR"
     gene            complement(22546..23085)
                     /locus_tag="PLP58_08585"
     CDS             complement(22546..23085)
                     /locus_tag="PLP58_08585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ08609.1"
                     /translation="MNARILPLSLRLPCLALMSAFVLAGCGADEADGAKKTAVPETPW
                     EQVKAEAARPGDPKFAEKMRRWEADLAQATFPVLVPDDVAVLSRLELNVKSHLASVLA
                     VFATHRVQMTAVRSGRADAPDAVALRKDRAELEFVRWGTTYKISVVCAGASGPDPAPC
                     ADDKLVRALRASLVWANPR"
     gene            complement(23128..>23680)
                     /locus_tag="PLP58_08590"
     CDS             complement(23128..>23680)
                     /locus_tag="PLP58_08590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ08610.1"
                     /translation="LWGGPAMMLALHALPLSSGAKIAGSALVLALALLAWSSRLRGWV
                     QNLLRKESAAGVIFALGCTTFARFSHQGADDPVHWLELMLLAFLFTGNLTVIAQQETP
                     HADRRGQGVFLIGSAVFCAVLGHGGFFGGSSESLRAISLLVIAGLLLHLLLWNAARHR
                     SAEIFRVLADAALLLPPAWVWLR"
BASE COUNT         4210 a         7882 c         7553 g         4035 t
ORIGIN      
        1 agttcgcttt ggccgagcag ggcggggtct ggcgcggtga ccagcgcctt gaggctggga
       61 gccagctccg cgtccccgct cacattcgag aggcgcggca gttgatcctc cagcccgccg
      121 agaaagggcg cgtgcggcgg cggcacgtcc tgccactgcg cctccatgcc cagcaccacg
      181 ctggggaagg cgagcagtgc ctcggcgagc gcgggcagga agtcaggcgc gggctgcccc
      241 cagttcaggg gcgcggccac cacgagcaca tccggctcaa aaggtgccag cccctggagg
      301 attgtccgcc agtccagcgg cggcggcggc caggcggcgt attccgcgcg gtcccgctcg
      361 cgcagggtca ccagcgccac ttccggcgcg gcggcggggt cgggggtctc cagccgctcg
      421 cgcgcgttgg cggtgaggaa gtcgaggaac cactcgtcca cctggcgcag gcggcccgcc
      481 tggcgctccc gctccaaatg ccagccgccg ccgccgaggc agagcagcag gataatgagg
      541 cggagggccg tcatgaatga aggggcggtc cgggggacag cagagggggc agaaccgcgc
      601 gcggcttatt cgactccggc tttgagcacc gcttcgagct gggattctgg caggctcatg
      661 ggatggccct cccagacgat cttgccggtt ttgtccacga tcatcgcgtg cgggatgccc
      721 ttgatctgga agtcctcgct gagcttgccg taggtgtcat gggccacggt gtattcgatg
      781 gggatgtttt tttcgaagtt gcgcaccgtc tgccggtcct ccttcgtcac cccgatgatc
      841 tgcaggccct tgtccttgtg ctttttgtac aactcgttga gatgcgggat gctcgtgcgg
      901 cagggcgggc accaggtggc ccaaaactcc agcacatagg ccttgccctt caaatccggg
      961 gaggcgccgg aaaagcgcac tttcaggtcg gtgatgaccg tgccgacatt gtccttgccg
     1021 gcggtggcca gcagcgtggt ggaggccagg gcggtcaggg ccagggccag gaggattcgc
     1081 gctgttttca tgggatgtgg attggttttg agttcggagg ggattcaacc cggattcgcg
     1141 cgccgttgtc gagcacaatt ttcaccgggc tgccgggcga gcggcggaga gcggcttcgc
     1201 cgccgccttc acactgaagt cgtcataatg gacatccacc gcgttggtgg tgaggctgac
     1261 gagggacttc gtggcgtgcg ccacgccctc ggagctgaac tcgcccgcct ccttgccgtc
     1321 aatgagcaca aaaatcgtgt cgcccttcgt gcgcgccgtc agcgtgtgcc acttctgcag
     1381 ctccagcttc accggcacgc gcttggtttt ggactccagc atcttctttt cctcatcggt
     1441 gagctggttc gccttcttgc ggtcgtaaag gccgttttcc aaagtgaagc cgcccgtctt
     1501 cgcgtcgctc atctgcacct gcgcggggct caccgtcacc tggcagatgt ggcccgcgtg
     1561 cgcgcctttg taatccttgt cgtcgaacca gacattgaaa ctcttcgccg cctcgctcac
     1621 gaagcggaac ttcacctcca cctgcaaatc ctgctcgccg gggaacttga tgttgtccac
     1681 cgccgaatgg tccgatccct ccggggtgat gcccaccagc acgccatcct ggatgacgct
     1741 gccgctcttg taatgccccc agccctcctt gaaggcgccg ccggagaaat cgtcctcaaa
     1801 aatcacccgg ctgtaatagg ccgcctcctt gccctgcttg cgctccaggt cccagagccg
     1861 cagccagtcc tgatccgcct gggagaggct ggcgagcggg atctggaagg gcttcaggtt
     1921 cacattcacc ttgagcatgc cgtcgtccgt cagatcgagc agctcggcct tgatggtcac
     1981 gccatcgagg ttggtcagat cacgatactc gcgcgcgctc agacttgaga caccgagggc
     2041 gagcagaaaa aaaagggtgg gcttcatcat ggcaacgtca tgaacgaggc atcccagcct
     2101 ttgttagcgg caagatcacc ccggcctcag cacgctcttc accacctcgc ccttgtgcat
     2161 cttctcacag gcttcgtgcc attcggtgat gggccagacg ccgccgatga tgggtttcac
     2221 atcaaactgg ccgccactcg cgaggacggg cagcgctttt gcaaagaccg caaaagaggc
     2281 aggagaatga caaacaacgc gttatgggcc tgtcatgatg aaccgccgcg ctcttcttgc
     2341 ttccctcgct gccgccaccg tttgccgtgg agcggacgag cggcgttttt tcacggtcgc
     2401 gcagcggaag gggcgctggt ggttcatcac accggagggg aggccgtttt tctctatcgg
     2461 gctgaatcac atcgatcccg cgccgctgcg ctacgcgtcg aatggcgacc tgtgggagcg
     2521 caaatatggc aactcgatgg aaaagtggct caaggaggcc gtggcaccgg atttgaaggc
     2581 ctggggcttc aattcggtcg ggtggacgca ggaggtggtg acgcggggaa gcacgaatca
     2641 tcgtcattcg cgtgcgttca cgtttgagga gtatcagtgg ctcgggctgc cgtattgcca
     2701 tcagctgccg ttcgcggatt ttcatcagtg ggaggcggag acacggcatc cggacttcga
     2761 cgcgccggaa ttcgccgagt ggtgtgacta tgtggcccgc gagcactgcg cccgactgaa
     2821 ggatgatccg aagctcattg gctattttta cattgattgc cccacctggg ttcacacacg
     2881 gcccgagagc gcatggaaag gcccgcttta cgatccggag aagctcaaga cagaggcggg
     2941 tcgcaaggaa ctgcacgaac tggccacgaa gtggtatcgc atcacgcacg acgccatccg
     3001 ccgctacgac aagcaccacc tcatcctcgg cgaccgctat gaggccgcca accccatcgc
     3061 cgacgaggtc atcgacgcgg cgctgccgta tgtcgatgtc ttgagcttcc agcacttcag
     3121 cgggccggac aaggtcacgg cgaatctgaa ccactggcat caaaagaccg gcaagcccgt
     3181 gctcctcgcc gatcacgcca tccagatccg cgaagccgac ggctccggcc gccacgacgg
     3241 cgaaggcgat gcccgcgtgc tcgccgcgct gcgggagacg cccggctgca tcggctacca
     3301 cctctgcgga gcctacctgc gcaacgaagc ccgcaaacgc gccctgcgtg atgccgccga
     3361 aaaaccggat gaagcggcac tggaggcgat cacgcgggcg aatcgagaga cggcggcgtg
     3421 gatggagcgg gtgaggtgac gattacggaa atgccgcgag aaactcctcg ggcgtcatca
     3481 ccggcatcgc cactccgaca aaatcaatcg tgttgcgtgt gatgaacaca tcagccctgc
     3541 cgctcaaggc gcaggccatt tgcaaggcgt cttcaaaatc gtgaatgccg tggctccgcc
     3601 aatctgtggc gtcaggttga ccgacggtgg gaacttccag catcagcagc agctcatcca
     3661 tcatggccga cgtggcttcg gaggcatttt gcttcgcgtg gtagtaagcg atgatcggca
     3721 gcgtgtgcca tgccacaaga ccttgatgga cacctgagtc acacaggttc agaaggcgtt
     3781 cactcccatc cttgccggga cgcgcttgtc cgcttgtctc caggacgagg cagtccagca
     3841 ggacattggc atcaagcatc acgcgcatga cagctcagcg caggtgtttc tcgttgatgt
     3901 gtgccagcca ggtgtctccg gcatcctcga tcttcttcgc tgttccgctc cacttctgaa
     3961 caaagcccgc aggcttcttc ttggctggag aattagcctt cgcaggcaca gaagcggcca
     4021 cctcgaagga aatggagtct ccctcgacat gaaatttcac ccaatgatca ccactgggca
     4081 gtcccagtgt gccgagaggg gtggattggg tcagtgtctt catggtgatg agattaactt
     4141 cggttcagga aggcgtcaaa cagggtgatc acaccggccg cagcacgctc ttcacgactt
     4201 cgcctttgtg catcttctcg aaggcttcgt gccattcggt gacgggccag acgccgccga
     4261 tgatgggttt cacgtcgaac tggccgctgc tcatgagggc gatgacgcgc tcccagatgg
     4321 gccagttgtg gctgaagctg ccttgcagcg tgatgttctt ctgcacgagc gggtcgatgt
     4381 tgaagccgag cggttgcggc ccccagccga ctttggaaat ccagccggcg gggcgcacga
     4441 gctcgagcgc gattttgagc gtgatgctgg caccggcggc atcgatgacg ccgtcgcaac
     4501 cgaggccgtc gcgttccatg gcccagggtt tggcatcgcc gatgatgact tcgcagccgt
     4561 agtgcttgcg ggcgagttcg aggcgatgcg cgtcctgctg gaggccgacg atggcgactt
     4621 gagcgccgca ttgtttcgcc atcgcggcgc agaggatgcc gatggtgccg gggccgagca
     4681 cgatgacgcg gtcgccgggt tcgatgcggg cgtttttgac gacggcgttg taagcgacgc
     4741 aacagggttc ggtgaggcag gcctgctcga agggcacatg atccggcata tgatgcagga
     4801 tgcggctggg cacgcgcaca tacttcgtca tcgcgccgtt cacgccgtag ccgaagcctt
     4861 tgcgcgtgtt gtcgaggttg tagaggccgc ggcgcgtcat cgggttgtct ttggagatga
     4921 tcgcggcggt ctcggagacg acgcggtcgc cttccttcca gccttcgacg tccttgccga
     4981 cctcgatgat gtgtccgccg aactcatggc cgagcacgac ggggtaattc accggccagg
     5041 agtgatcgga ggtccactga tgcagatcgg agccgcaaac gcccacattg gcgacttcga
     5101 ggagcacgtc ctcggcgccg atggtgggct tttcgatctc acggatttcg acggagcctt
     5161 tttcaggcgc gaagttgacg acagcggcgg atttcatggg tggaaaagtt gaaggatacg
     5221 cggtttcggg cgcgctccac gacaaagagt gggtgatttg cgacaggcgg ggcgctggaa
     5281 gatgtcagac cagagcgggg tggttcgtta aagcggtgac atccgcatga acatcgccac
     5341 caaacgccat ctcgaccgcc gcgcttttct gcgtgggact ggggccgtgc tttcgctgcc
     5401 cttcctggag gcgatgctgc ccgcctttgc cacgcgcgcg caggccgccg cagggactcc
     5461 gccgccgcgc ttcctggcca tgtgcgccac gctgggcttc cacacgccgt atcttttccc
     5521 caaggagact ggcgcggatt acactttgac gccctacctg gagccgctgc gggatttgcg
     5581 ctccgatttc agcgtcattt ccggcctcaa tcacgagcag caaaacgggg ccaacggcca
     5641 tacttcggag atgacctggc tcacggcggc aaagcacccg ggccttgccg gattcaaaaa
     5701 caccatctcc attgaccagc tcatcgccga gcgcatcggc actcagacgc gcttccccag
     5761 cctgatcctt ggcaccggca gcgagtcttt gtcctggtcg gcaagcggcg tgccgctgcc
     5821 tgcggaaagc tccccctcgc gcgccttcca gcagcttttt gtggacggca cgcctgccga
     5881 ggtggccgcc caggtgcgcg cgctgaaacg cgggcgcagc atcctggaca cggtcatggg
     5941 ccaggcgaaa aaactgcgcg gcgagctggg ccaccgcgac caggagaagc tcgacgaata
     6001 cttcagcgcc gtgcgtgatc tggagggccg gctcgtgcag aacgaggagt gggcgcagcg
     6061 tccgaaaccg aaggtggacg ccaaacctcc gaccgacatc cagagccgca cggacgccat
     6121 cggcaagatg aggctcatgc aggacctcgt ggtgctggcg ctgcagaccg actccacgcg
     6181 caccgtgacg atgcgcctga gcggcatgaa cgcggtgccg gaaattccgg gggtgaaaaa
     6241 cgactggcac aacctctccc accacggcca ggaccccgcg aagatcgagg agctgaaggt
     6301 catcgagctg gccgaattca ccgccttcgc ggacttcctc gccaagctca agcagacccg
     6361 ggaaagcgga cgcccgcttc tggacagcac cgccgtgctt ttcggctcga acctcggcaa
     6421 cgccagctcc cacagcacgg ccaatctgcc gctcgtgctc gccggcggcg gcttcaagca
     6481 cggacgccac cttgccatcg agaaggacaa gcatgtgttc tccaacctct tcgtttccct
     6541 ggcccagcgc atgggcgtgg agacggaccg gttcggccac agcaccggcg tgctggacat
     6601 caaccaggcg tgaggtgatg cctggagcag cacatcgctt gccagcggcg gccaggcatg
     6661 ggaagatcac gtcatgagca tcgaagcagc ctttcatcgc ggcatcgttc cgcttctccg
     6721 attgcttctt cccggcaagg aggatgcggt tctggggacc actccggaca aggctttgca
     6781 cgagcggatc gaatggctgg ccgcccgcaa caccgagggc gaactcactg ccgaagaatt
     6841 ggccgagtat gagggctatg tccgcgccaa caaattcgcc gctgtgctgc gccgggaagc
     6901 tctgcggatg aaagaggaga caggcaggtg actgagaaag tccggcgaaa ggtccgtgaa
     6961 cgcgctggaa accgctgtga atattgcgga ctcaaccagg aggactcgcc tctcgcctcc
     7021 ctccatatcg agcacatccg cccgaaaaag cacggaggga cagatgatga gaccaatctc
     7081 tgtcttgcct gcatagactg caacttgcgc aaaggcgcca acctcacggg cattgacccg
     7141 gacacagacg ccatcacgga actttttcat ccgcgccgcg acatgtggcg ggaccatttt
     7201 gaatggcagg gcattcgaat catcggtcgt tcgcccattg ggcgtgcgac tgtcgttgtg
     7261 ttaaatctca acagtgaaga ccggctggct ttgcgtcttg tttgaaggct catcgcgggg
     7321 gcgggaatcg tcctgtcgcg gagcgcggca cttgccgcgt cagattgacg gcgggtgatg
     7381 cgttgagaga ttgcttatga cgctccgcgc cgcccttcga cttccgagtc ttctctgcct
     7441 cctgctcgcc agcgcccacg ccgccacgcc cgccgcgcgg ccctttctgg accggcactg
     7501 catggactgc catgacgcgg agatgaaaaa aggcgggctg gatttgtccg cgctgtccac
     7561 cgatggcgcg gacgccgccg ccttgaagaa atgggtgcgc gtctttgacc gcgtggccgc
     7621 cggggagatg ccgccgccga aaaaggcgcg ccccgcggcg gaggagacga gcgcattcat
     7681 tgcggcgctc ggctccgacc tgaccgcgaa gtccgaggcg cgcaaaggca ccgtgctgcg
     7741 ccggctgaac cggcaggaat atcaaaacac gctcaacgac ctgctcggcg tgcgcgtcag
     7801 cgtggtggac atgctgccgg aggacgggcg cgcgcacgga ttcgacaccg tgggggaggc
     7861 gctctccatc tccggcatcc agatgcagcg ctacatggag gccgccgaga cggcgctgaa
     7921 cgcggccttg atggccgggg agaagccgga actcagaaag accgcagcca cactcgacac
     7981 cgggcgcaac aaggacaaca tcggcaagca ctggctgaaa aaagccgacg gtgccgtggt
     8041 ggtcttcaat gagggcggct ttcccagcac ggtgatcccc gacctgcgcg cccatgccac
     8101 aggcacttac aaggtgcgtg tcacgggtta cgggcaccag gtggaggagc cggtcgtttt
     8161 tgccctgcgc accggcagtt tcaatttccg caacaccgac acgcgcacgc acagcttcca
     8221 cgagctgccc ccggacaagc ccggcacggt ggaggtgacg ctgcacctgc cgcagggcca
     8281 cggcatccag gtcagtccgc agggccttta cgggccggac gggcactcgg cggtgaaaga
     8341 cgggccggac aagtatcccg gcagcggcct ggccctgctc tcggtggaga tcgagggtcc
     8401 gctcattgag gaatggccgc cgcgtggaca aaagctgctg ctcggagatg cgaagctccg
     8461 cgaggtcatg cccgagaaac cctggatgcg tggcaaggcg ggttacaaac ccgtcttcac
     8521 cgccgactcc gccgatcccg ccgccgatgc gcgccggctg ctgcccgcct ttgccagcgc
     8581 cgcgttccgc cgccctgccg atgcggctac ggtggagcct tacgtccggc tttttgacgc
     8641 cgagttcgcg gaggggaagg acttcctcgc cgccatgcgc acggcagcca cagccatctt
     8701 gtgcgcgccg gattttcttt ttctcaagga accctccggc gcgctggaca ggctgcaact
     8761 ggcctcgcgc ctgagttact tcctcacccg cggcgcgccg gacgcggaac tgctggcggc
     8821 ggacctcgcc cagcccgccg tgctgcgcgc gcagacggag cggctgctgc aaagccccgc
     8881 gctggagcgc ttcgtcaccg atttcaccga cggctggctg aacctgcgcg agatcgagtt
     8941 caccacgccc gacaagcagc tctaccccga atacgacgag ctgctgctcg actccatgct
     9001 gcgcgagacg cgcgccttca tcaccgagct gatccgcggc aacctcagcg ccgccaatgt
     9061 catccacagt gattttgcca tgatcaacgc gcggcttgcc cggcactacg gcatccccgg
     9121 cgtcagcggc cagcacatcc gcaaaatgcc gctgccgccg gatagccggc gcggcggcgt
     9181 gctgacccag gccagcattt taaaggtcag cgccaacggc accaacacct ctcccgtggt
     9241 gcgcggcgtc ttcgtcatgg accgcatcct gggcatcgag ccttcaccgc cgccgcccgg
     9301 tattccgggt gtcgagccgg acatccgcgg cgccaccacc ctgcgcgagc tgctcgacaa
     9361 gcaccgcgac agcgagacct gcaacggctg ccaccgtgtc atagacccgc ccggcttcgc
     9421 cctggagagc tatgatgtca tcggcggctg gcgcgagcgc ttccgcagca tagaccaggg
     9481 ggaaaaagtg gacctcaagg tcacaggccg caaagtgcgc taccgcctcg gcccgcctgt
     9541 ggacgcggcg ggcgagctgc ccacgggggc gaaattcgcg gacatgggcg ggtttcaaaa
     9601 actcctgctc gccgggcagg accgcgtggc gcgctgcctg ctggaaaagc tcctcacctt
     9661 cgccaccgga cgcgagatgg gcttctccga ccgcgccgag ctcgaccagc tcaccgccgc
     9721 ctcccaagcc aaaggccacg gtgtgcgaga aatgatccac gccgccgtgc aaagcggcat
     9781 tttccgccgc aagtgactgc ggtgtgaaag ggcggactca tcccgccagc gccgcccagg
     9841 ggccgcgctc cagcacgatg ggcggcacaa aatcccgcca gcgcctgtcg ccgccgagga
     9901 tcatctggcg gacgttggag ctggtttgga agaggcattc ctccagcccc tcgcggattt
     9961 cgtggacggc tcccgtgtcc agcaggtagc gcagcagatg gtcctggccg cgggtgacgc
    10021 gcgcctggct ggcggtgcgg atttttcccg tgagaggatc gcccgccgga taaacgtaga
    10081 gccttacctg gttgcgaaag agccgcccga aggcctccat cagccctcct tcgaggtcgg
    10141 cataccagcg ctcgttgaac agctcctcga aaagcggcag gccgaggatg atgccgatgg
    10201 gttcgcgcgt gtggcgcgcc aggtagccgc tgaggcggtg aaactcggca tacttggaga
    10261 cgagcacgtt tttgcccagc gcctggagga tgtcggcgcg cgcgaggaag ttcccgtact
    10321 ccaccgcgca gtcgtccatg cagagcaggt tatgcatggt gatctcgagg atctcgacat
    10381 ggccgagcgc cttttcgccg aagtcctggt caaaggccgc cgcgccgcgc tcgatcatgt
    10441 ccagattcac tcgggtgatg gggtcgaaac tgccgcggtt gagcacgacg ggtttgttgt
    10501 agagcacatc cgaggcctga agaatctccc cgctggcacc gaagagggcg gcgtccgcca
    10561 ggtcgctgcg cacgagttcc agcgccacga tgcggtcctc gaatccgggc ttggcgaagg
    10621 cgggaccgat gaagtccacc atgtctatct ccaggcgctt gcggcgcagg tcgtcgagca
    10681 ggcttttgag gaagacggtc agctcgtcac ggtagtggtg cgcggcgtag agcaggttga
    10741 cgccgaggat gcccatcacc tcggactggc ggtggttggt gtcatcgagc aggcggacat
    10801 gaagctggat gtcgttgggc gggcctcccg gctcggtctg aaagcgcagg ccgagccagc
    10861 cgtggcattc ctcgttggtg tcgcgccagc cgcgcgcgcg caccgtgttg gcaagggcga
    10921 agaaggcggc gtccgcgccg cgcttggcat ggagccgctg ctgcacgatc tggaactcgt
    10981 ggtccagcat cgccgccatg cgctgacgcg agacatagcg ggcgctcttg ccgtagatct
    11041 cgtcgctgaa ggtcatgtca taggcggaca tcgtcttggc cactgtgccg gaggccccgc
    11101 cggcacggaa aaaccagttc gccgtctcct gccccgcgcc gatctcggcg aaggtgccgt
    11161 agatgccgcg gtcgaggttc agcttgaggg ctttttccag cgtgccgggg cggttggggg
    11221 agtcgttcat cgcggcgaac ctagcacaaa acagccgcgg ccaaacccgg acttggtgtc
    11281 acatgcgccg gcacatgccg cgcaatcttt gtcgttgacg aaaacctccc cgccgggctt
    11341 aatagcgccg cgcggcctca accgcgcgac aactttggtg agatggctga gtggtcgaaa
    11401 gcacaccttt gctaaaggtg cgtagcctta tccgctaccg agggttcgaa tccctctctc
    11461 accgccactt ccctccgcac ggacggcggg tgtgacgggc ggggtttgca gtcccggcat
    11521 tttttgggtg aagccgcgcg ccgcctgggg taaaacaccc gccgtgtccg cgcgcatcct
    11581 cccatcccgc ccgcttcttt ccgccctggc cgcgctgtgc gccgcctgcc cggccggcgc
    11641 gcaaacaccg aacgcgcagg acgggcacac cggcagcctg ggcatgaaat ttgtgcgcgt
    11701 gcccggcacc acagtgcatt tcgccatcca cgagaccacc gtggcgcagt ggcaggcgtt
    11761 cgtgagggag ggcggccacg cctgggacta caagccgcac tttgagcaag gccccgacca
    11821 tccggctgtg ggcgtcacgc tccaggacgc gcgcgccttt tgcggctggc tcacggagaa
    11881 agagcaaaag gagggccggc tgaaaagcga gcagggctac cgcctgccca cccgcgccga
    11941 gtgggacgcc gccgccgggc tgctgcgcac gcgcaagccc gaccttacgc tcgatgacaa
    12001 gctcgccgac gaccgcgcct tcccctgggg ccaggagtgg ccgccgccca aaggcgcggg
    12061 caatctcgcg gaaggcgaga tacccggtta cgaggacggc ttccccttca ccgcgccagt
    12121 cggccagttc aaacccacgg ccgacgggtt gcacgatctg gcgggcaatg tctgggagtg
    12181 gtgctgggat cccgagatcc gcgccgagca gcatggcgtg ctgcgtggcg gctcgtgggc
    12241 ctacttttac cccgaaagcc tgcgctccgc ctatctctac accgtgcccg ccgccctgcg
    12301 catgcccacc atcggcttcc gctgcgtgta tgaggacaag gcgcgcaccg ccaccctgct
    12361 cgccgccatg aaggccgacg cggacaagct ccgcgccgac cgccgccagg ccctcaccgg
    12421 cgccgaggtc agccaggacg aggtggccgc catgcgcaag cgcttcgcca ccggcggcga
    12481 agcagccaac ctgccggacc ccgccagcct caagcccgcc gccccaggcg cgcgccatct
    12541 caacagcctc ggcatggacc tcgtcccgct cggcagttcc gcgctcatcg ccgccatcga
    12601 gacccgcgcg caggactacg aggcctttct caaagccagc ggccgcgcct ggaccaaaaa
    12661 acccaccttc ctcatcagca gcgcgcatcc cgccgtcagc gtgagctggg acgaggccgc
    12721 cgccttctgc gcctggctca ccgaaaaaga gcgcgccgcc ggactcatcg gccccggcgc
    12781 ctcctaccgc ctgccggacg acctggagtg gagccgcgcc gccggactcg aggacgagac
    12841 cggggccgat cccgccgagc gaggcaagtc cccctccgca cactacccct ggtccgccac
    12901 cggcgagttc cccccgccgc ccttcagcgc gaacctcgac gccggccgca tccccggcta
    12961 cagcgaccgc cattcctaca ccgcccccgt caccaacgac cccgccaact ccctcggcat
    13021 tcacgcactc ggcggcaacg ccgccgaatg gtgcctcgac gcctggccgg gcgcggaaaa
    13081 cgagcgcgtc atccgcggcg gctcctggct ctccttcgac aaggaccagc tccgcaccgg
    13141 ccaccgccag cacgccgcca gggacagcgc caccgccggc acaggcttcc gcgtcctgct
    13201 cgacttcaac cgcccctgac ctcccacggc catgcccgac cccagcccag acgatctggt
    13261 tctgaacccc cgcccctggt tcacctggat gctctccgcc ctcggcgtgc tcggcctcgc
    13321 cgcctggttc gcggggccgc cgctggcgga atgggggcgc gaaaagctgg cggagcactt
    13381 catctcccag gccgagacgc acctggataa cctcgagttc gccgcctgcg ccaccgccat
    13441 ccgctccgcc cgccgctggt ccatgcaaaa cggccgcgtc ctgcgtgtca gcatcgaatt
    13501 cctcgacaaa gccggcggcg aggacaccgc caagctccac atgctgcgcg agctcgtcga
    13561 atccggccag gccaccctgg ccgagcgcgc ccgcattggc aggctcctgc tggcgcgcgg
    13621 cgagattcag ggcgcgcgcg aggagctggc gcgcttcacc gacgaggaaa aaagcgccgg
    13681 ccccgtgctc gagctgcgcg ccggcatccg ccgcgccgag ggcttccccg acgaggccga
    13741 ccgcctcctg caccaggcgc tcaccctcac ccaggcggac gatcccgacg cgcgtctcag
    13801 gctggccctc atggacctgc gcgcgcctta cccggagacc cggggcagcg cgcgcgagag
    13861 actctttgac atcgccgctg gcggggacac gcgcgccctc caggccattg agcgcctcgc
    13921 cgccgacagc gagatctccg gtgccgaagc cctgcggctg ctcgacctgg ttgaaaagca
    13981 ccctctcaag accgagcagc ggcggctcga caccctcgcc gccctcctgc gcgtccgacc
    14041 ccaggagcgg gagcgcatca tggagcgcga gacccgcggc gcggcggaca tggacgagga
    14101 gcagctcgcc cagctcgcga actggctcgt ggcccgtggc gaggccgcgc gcgtcatcga
    14161 gctcgtgccg ctggagcgcg cgcgcaaatc caccaagctc ttccacagcc acatcgccgc
    14221 gctcgccaaa ctggagcgct gggacgagct ggaggccgaa ctcaagcgca cctccggcgt
    14281 cattcccatg tcgcgccccg gcatccatct ctggctggcg cgcgccgcca accgccaccg
    14341 cgacggcatc ccgcaagtga ggcaggcgct ccaggccgcc ctcatggccg ccggggaggg
    14401 atcggacgcg gcgtcctccc agctcgccct ggccgtggcc gaggaaaccg cgcagtggga
    14461 cctggccatc caggcctgcg agggcatcgc caagcaccac ccgcgcaccc gcgccgccat
    14521 gatcgagaag gtgctcgaca tctccctgcg cgacaaagac acccccggcg tcgtcagcgc
    14581 cgccgcccgg ctcgccgagc tgcagccgca aaacgaccag gccgtcttcc gagcgcgctg
    14641 gttcagcctc gtcgccggcg agggcatcga gaccgccatc caggcgctcc aggctgagag
    14701 cgcccgctat ctccggcccg gccctcgcca cctgctgctg gccctggccg cgtggcgcat
    14761 gggggacctg gagcagacgc gcgcgcacct ggagcacgtc accgaccctg gcgagttgag
    14821 cgtcgggcag cgcgcagtgc atgccgggct gctttccgcc tgcggcagcg tcggccccgc
    14881 cttccagctc gccgagcgcg tgccgcccat cctgctcctg cccgaggagc tgcgcttttt
    14941 gcggcgcgcg ctttgagcgg ggaatccgct ggcggcgcag ttttttcttg ggattggcac
    15001 agcgggccgc tatgacacgc gcatgaattt ttcagaattc gccgccgcct tctcgggata
    15061 cgtctggggc acgcccctca tcgtgctgct tttcggcacg catctcttcc tcaccttccg
    15121 cacggggctc atccagcgct acatcccgcg cgccatcaag atttccttca cccggagcaa
    15181 ggagggtgag ggggatgtgt cccagtttgg cgcgctgatg accgcgctgg cggccaccat
    15241 cggcgcgggc aacatcattg gcgtggcggg agcggtggcc gcgggcggtc cgggcgcggt
    15301 gttctggatg tggctgaccg gtgtcttcgg catcgccacc aaatactccg aggcggtgct
    15361 ggcggtgaaa taccgcgtgc aaatgccgga cggcagcttc gccggcgggc cgatgtatgc
    15421 gctggagcgc ggcctggggc agaagtggct gggagttgtc ttcgccgtct tcacctgcat
    15481 cgccgccttc ggcattggca acatgttcca ggccaacgcg gtggtggaga cgctcaagga
    15541 gtgggtcaag cccggcgccg aggcggacgc ggtgctgcgc tgggtggcgg gtctggtgat
    15601 ggcggccatc accggcgcgg tcgtcctggg cggggtgagg agcatcgcgc gggtttgcgg
    15661 ggtgctcgtg cctgtgatgg cgctgggtta catcctgggc tgcgtgctca ttctgatcaa
    15721 aaacgcggcg ctgctgccgg gggcgctggg ggagatctgg gatggcgcct ttgccgggca
    15781 ggcggcggtg ggcggctttt tgggcgccac gatccgccag gccgtgcagg ccggcatcgc
    15841 gcgcggcctg ttttccaacg agtccggcct cggcagcgcc cccatcgtgg ccgccgccgc
    15901 gcagacgcgg aatccggtgc gccaggcgct ggtctcctcc tccgccacct tctgggacac
    15961 cgtggtggtg tgcgccttga ccgggctggt catcgtcagc agcgggcatt ggaaggaggg
    16021 gctgcccaaa gcggcgctga ccaacgccgc ctttgaggag ctgcacacca tcggccccat
    16081 tttcctgctc ttcggcctca tgaccttcgt cttttcctcc atcctcggct ggtgctacta
    16141 cggggaaaaa gcggccgaat acctcgtcgg gccgcgcgcg gtgaagccct accgcatcct
    16201 gtgggtgatc atggtcctgg tcggcgccgt ccagccggcc gcggcggtga tggacttctc
    16261 cgacgccgcc aacggcctca tggccgtgcc caacctcatc tgcctcatcc ttctgagccg
    16321 tgtcatcgcc gcggaaaccc gcgcccaccg ccacgagctc accggcaccg actgagccgt
    16381 gaccatgcgg cagattctgt ctccggtcgt tttttcccga tcaagccggc aaaacccagc
    16441 cccccgccat gcacacagaa cgcgcgctca tcctcgccag tctttgtctg gcattcgcct
    16501 cgctggacct tgccgccgca gcgccagtac tccgcgcggg cgccgccacg agcaacatca
    16561 cgccgaccat cgccgctgtg cctgtgggtc gcacgagccg accgccagcg acgcatgtgc
    16621 atgacgattt gcacgcccgt tgtctcgtgc tcgacgatgg ggaggcgaag ctggcactgg
    16681 tcgtgtgcga tctgcggcac atttcggccg aggtcgcggc tgcggccaag gtgatcatcg
    16741 aaaagacgac cggcatcccg ccgcagtgcg tggtcatctc ggccacgcat tcgcacacca
    16801 cgggtggcgc gaggctggag gagcgcgagg gcgagccgta ttttgaatac agcgcgtttt
    16861 tgacccggcg gatcgctgat ggcgtgctgc gggctcacaa tcaactggag cccgcgcaga
    16921 ttgcctgggg tgtcgccgag gagctgacgc aggtcttcaa tcgccgctgg ttcgtgatgc
    16981 cggagcgcgg cccgatctac ggtgcgcatg acaacgtcga aaaggtggac acgaatcccg
    17041 gatacaaagg cctgctgaag gccgccggtc ccgtggaccc gcaaatcacc ttcctttcgg
    17101 tgcgctcaac ttcgggcaaa ccgatctcgc tcatggcctc ctacgggctg cactacgtcg
    17161 gcggtgtgac gccgaacacg atctcggcag actacttcgc gattttcgcc gaccgcgtgg
    17221 gcgagctgct cggcgcggac cggcatcaag acccgccgtt tgtcggcatc atggccaacg
    17281 gcaccagcgg cgacgtgaac aatctggagc gttactccga cgaagaactg gcaaagcgtg
    17341 gtccgcagcc gaagaagaag tatcaaccct tcgaaaaagc ccgcgaggtg gccgacttgt
    17401 gcgccagcaa agtcattgag gctctcaaga ccgtgaaatg gcacgaccat gtgaagctct
    17461 cgtcggtgca gcgcacgctg atctttgagc gacgctatcc gacgaaggaa gaggtcgcgt
    17521 gggcggagtc ggtgaaggcc cgacgcatca agccgatgag caccagccgc tacagcacct
    17581 acagcaccgt gctcgcatac gcctcgccgg acatgccgcc gaatgtcgat gtcatcgtgc
    17641 aaacacaccg catcggcgac ctcgccgttg ccgcgatgcc gttcgaggtc ttcgcggaaa
    17701 tcggcctgga attgaagcaa cgcagcccca tccagccgct catgaacatt tccatcgcca
    17761 acggctccca cggctacctg cccacgcccg aacagcacaa gctcggcggc tacgaaacct
    17821 ggatcggcgt caacaaagtg cagctcgacg cctcggtgaa gatggtgaac gccctcgtcg
    17881 agatgctcgg cgagctggcg gcggagaagc cgtgagcaga tcgaaaaccg ccctcacgag
    17941 cagtctggcg acatccactt cgcaagaatc accctgccag attgaacttc acgttatgaa
    18001 ccgctaagcc ggcaaaagtg atccggtctg agttcatcaa actggttggc cctcctggga
    18061 ggcgcattcc gaaactattt tcaccttttt caccaccact tcagcgcggc ttctgtagat
    18121 catcgttgaa caccgctttc tcgcatgaat ccaccccgca gtcccgcctt tcccgccacc
    18181 cgttggagca tcgtcgccgc cagccagcgc ccggatgatc cccgtgccag caccgccctt
    18241 gctgacctct gccgggacta ctggttccct gtgtatgccc acatccgttc tcagggccgg
    18301 agccagcagg acgccgagga tctcactcag agtttcttcc agcgcatcct ggagaagcac
    18361 acgctcaccc acgccacccc ggagcgcggt cgcttccgca acttcctgct cgccagcgtg
    18421 aaacactttc tcatcaatca gcacgagcac gcaaagtggc cgaaaccctc cgccagccct
    18481 cctcagccga catcaaggac gaactcgacg ccctgctgat gaatcaaacg tgaacccatc
    18541 ttttacctcc atttttacca gcaaatcagg ttcagatgcc catgtgcctt ttctctggta
    18601 aaagattgga aaagctgatt cccatccact tttgagccgt tttgtgattc ctcttcacga
    18661 ggacaaggaa agttcgcaag ccatgccccc gatgatagac tgtcgctcat gcacatcccg
    18721 acgctctatc tcgacacttc cgtcatcggc ggctacttcg atgatgaatg gaaggaagcc
    18781 acccaggaga tgttccagcg ggccgaactg ggtatgtatc agtttgtggc ctccgtcgtc
    18841 actgcccgtg aaatcatggg tgcgcctcca gaggtgcagg cgcactttgc cagcaccttc
    18901 actgatgaag cccagatcct ggagctgaac gatgaagctg agacgctggc cagtgcctat
    18961 ctcacggcag gagtcgtcac gcccaagtat gctgatgacg cccggcatat tgctgttgcg
    19021 acggtccatg ccctgccgct tgtagtgagc tggaacttca agcatctcgc caacatcaag
    19081 cgggaggctg gatttaacgc ggtgaacctc ttgcacggct acccgcaaat ccgtatcctt
    19141 accccactgc aactgctcaa cgatgaaacc gatgaagacc aaaacctttg atgccgtgac
    19201 cgaggcacgc cgctggaaag ccgatgtctc agcgaaaacg cagaccatga gccgtgagca
    19261 ggtgctcgca tttttcgaca aggataaagc aatgctcgct atggcacaga tgcgcagggg
    19321 aactggtgag cctgtcaagg cacgcgccta atttgcctgc ccagctaacc gctccggcca
    19381 gcccgtgcat cactgcaccc acctcaattt cttcggcttc ttctcctgcg gctgggttca
    19441 gcaagccgtc caacgccagc gcgaagccga tgaggtccaa gctcaagcca acggccaggc
    19501 aaagccagcg cagcaaccga cccacccagc gcgaacgcaa tgtccccccg ctgctcccga
    19561 ggctgcgccc gcgagggcct gtgcagccac cactacgaag aatgggccag cgccgtgatc
    19621 caccgcgatt ccatgaatgg ccggatttga ggagacgctg attcgactga gaagtcgaag
    19681 gcaccacagc agtgtgaatc ttgacctcaa atgaccgggg ggataatcat gccccatgac
    19741 aacgacaact ttatcccatg tgcttcccga actgatttcc aaactggccg tgatggggcc
    19801 tacgacactg gaggcggcgc ggagaagctg accggcagcg actgagccgc ctcatagcag
    19861 cgcatctggg gaggcaccca gatgcgctgg gaggcaccgt gggacgttgg gaggcgaccc
    19921 aaggcgctcg cggccaggcg tgatgtgttt cttttggctg gcgcggcgga ggctccctca
    19981 gtaataaggc gcatgactcc cgtgacagcc ctcctcctcg ccgcgctcgg cggctacctc
    20041 tgcggctcgc tgcctttcgg ttatctggcg ggccggctgc gcggggtgga catccgcaag
    20101 cacggcagcg gcaacattgg agcgacgaat gtgatccgcg tgctgggcaa aggcatcggc
    20161 attcccgttt tcatcctcga cctgctcaaa ggctggctgc ccgtgtggct cgcggcgggc
    20221 tggctgggaa cggcggaggg cgtggcgggc acggctttgc agtccgccgg cgtggtgctc
    20281 accggcttcg ccgcggtgct cgggcacatg ttcaccttct ggctcggctt caaaggcggc
    20341 aagggcgtgg caaccaccgc cggggtgctg ctgggcattc actggctggc catggtgggc
    20401 gggctgggcg tgtggctgat cacttatttc gtcacccgct atgtctctct ggcctccatg
    20461 ctcgctggcg tgggcgtggc gggcacgatg tgggtggaaa tgtcccgcag cgggcggaag
    20521 gaccccgtga tgctcggctt tggcatcctg gtcatggtgc tggtcatcgt gcgccaccgg
    20581 gccaacatcc gccgcatcct cgacggcacc gagccgcagg ctgggcggaa aaaggagacc
    20641 tccgccgggg agtgatccgg gcttgtgttt gccgcgcacg cccgccaaca tcggagcgca
    20701 tgaaattcca cctccccctg ctctccgccc tttgcatcgc cggactcatc gccttcgcag
    20761 ccgagcccga gaccccgaag gctctgcgcg tgctcatggt caccggcggc tgctgccacg
    20821 actatgaaaa tcaaaagatg atcctggccg agggcctcag ctcgcgggcg aatgtggagt
    20881 tcaccatcgt ccacgaggag ggaccggacg gaaagaagga caagacccac cgcatcagca
    20941 tctatgagaa ggacggctgg gcggcgggct acgacgtggt gctgcacaac gaatgcttcg
    21001 gcggcatcac cgacaacgct tttgtcgagc gcatcgcaag gccccaccac gagggcgtgc
    21061 ccgccgtgat gctgcactgc tccgcgcaca gctaccgcgc cgccgccacg gacgagtggc
    21121 gcaagtgcgt gggccagaaa agcatgagcc acgagaagaa ccgcgacctg ctggtgaaaa
    21181 acatcgcgcc cgggcatccc gtcatgaagg gcttccccat ggagtggctg gacaaggcgg
    21241 acgagcttta caagaacgag gtgctttacc cggacttcgt gccgctggcg aaggcctacg
    21301 gcgaggacac gaaacaggac caccacctcg tgtgggtgaa cacctatggc aagggcagag
    21361 tcttcggcac gaccatgggc cacaacaact cgacgatgga agacccggtc ttcctcgatc
    21421 tcgtggcgcg cggcctgctc tggtcctgcg gcaaactgaa cgacgacggc aaaccgctgc
    21481 ccggctacga gtcccggcag aagtgacccg gcgcggtcgc ctgcccgcac cgggtgcctg
    21541 aggcggcgtc agtcagcctt tttcttcgcg gctttcttgg ccggagcctt tttggcggcg
    21601 ggggctgggg cggcggcggc tttcttggcg gcgggctttt tctccgcggc ggcggacgtg
    21661 gccggagtca gggaggcgtg ccagtcataa acgtggatcc agtcatccga ggtctccgcg
    21721 cgcacatagt ctgtgtcctg gaggatgccg cgctggtgga agcccagcag ctcgtcagtg
    21781 gtgaaggggc cgaactcgac aaaggagcgg ctgagataaa gcaattgtgt catgtgggtg
    21841 tttttttggg tttgcgtttg ggggggcggg ggctttgccg ccaaaccaag agttgcacaa
    21901 gcaaaatcct tgccactttc ccgccgcgct catattcaaa gggaaacagc gcgtccaccg
    21961 catgagcgaa gaaccccatt tcatccatgc cccaagcccg cccggcgggg agccggagac
    22021 gccgccgcca tccggcaagg gcggcatcct caggcgtctg cgcgacagca agcgcgtgcg
    22081 gctttggttc gcggccaggc atttgaaact gaagcgggac taccggccaa tgctgctggg
    22141 ccggcccatg agccgtgagg aggtgggtgc ggcgggcgag gctctggccg cgcgctggct
    22201 ggcagggcat ggccgccgca tcctgcggcg caatcaccga gggcctttcc acggcgaggt
    22261 ggacatcgtg gcgcggcatg gccgggtgct gactttcgtg gaagtgaaga cgcgcaccag
    22321 cgcggagttt ggccgcccgg cggacgcggt ggacgcgcaa aagcgcctgc tcatccagcg
    22381 cggcgggcag gactggctga ggctgctggg acgcccgcgc gtgcgcttcc gcttcgacat
    22441 tgtggaggtg ctgctcatcc ccggcatgaa gccgcatgtg aacgtcatcg tgaacgcctt
    22501 ccagatgccc gacagctcca tgtccgggcg ctgagtccgg cgcgctcagc gcgggttggc
    22561 ccacaccagg ctggcgcgca gagcgcgcac gagcttgtca tccgcgcagg gcgcgggatc
    22621 agggccggat gcgcccgcgc acacgacgct gatcttgtag gtcgtgcccc agcggacgaa
    22681 ctccagctcg gcgcggtctt tcctcaatgc caccgcgtcc ggcgcgtccg cgcgtccgct
    22741 gcgcacggcg gtcatctgca cgcggtgggt ggcgaacacc gccagcacgc tggcaaggtg
    22801 ggacttcaca ttcagctcca gcctggagag cacggccacg tcatccggca ccagcacggg
    22861 aaaagtggcc tgcgccagat ccgcctccca gcgccgcatt ttctccgcga acttgggatc
    22921 tcccggacgc gcggcttcgg ctttgacctg ctcccagggt gtctccggga ccgccgtttt
    22981 cttcgctccg tccgcctcat ccgcaccgca gcctgcgagc acgaaagcgg acatgagagc
    23041 caggcatggg aggcggaggg acaggggcaa aatgcgggcg ttcatggcgc gatgatgccg
    23101 cattccagcg cgccggcacc cgctttttca gcgcagccag acccacgccg gcggcagcag
    23161 cagggcggca tccgccagca cgcggaagat ctcggcggag cggtggcgcg cggcattcca
    23221 cagcagcagg tgcagcagaa ggcccgcgat gaccaggagg gagatcgcgc gcagggactc
    23281 cgaactcccg ccgaagaatc cgccatgccc cagcacggcg caaaacaccg ccgagccaat
    23341 gaggaacacg ccctgcccgc gccggtccgc atgcggcgtc tcctgctgcg cgatgacggt
    23401 gagattgccg gtgaagagaa aggccagcag catcagctcc agccagtgaa ccgggtcgtc
    23461 cgcgccttga tggctgaagc gcgcgaaggt ggtgcagccc agcgcgaaga tgacacccgc
    23521 agccgattcc ttgcgcagca ggttttgaac ccacccgcgc agcctggacg accacgccag
    23581 cagggcgagc gcgaggacga gcgcgctgcc cgcgattttc gcgccgctgg agagcggcag
    23641 cgcgtgcagc gcgagcatca tggccgggcc gccccacagc
//
LOCUS       DAOMYJ010000062        23611 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Prosthecobacter sp. isolate AMR_MDS_5629
            NODE_775_length_23611_cov_21.221090, whole genome shotgun sequence.
ACCESSION   DAOMYJ010000062 DAOMYJ010000000
VERSION     DAOMYJ010000062.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867850
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prosthecobacter sp. (wastewater metagenome)
  ORGANISM  Prosthecobacter sp.
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Verrucomicrobiaceae; Prosthecobacter.
REFERENCE   1  (bases 1 to 23611)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 23611)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.30
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:34:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,742
            CDSs (total)                      :: 4,684
            Genes (coding)                    :: 4,676
            CDSs (with protein)               :: 4,676
            Genes (RNA)                       :: 58
            tRNAs                             :: 55
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23611
                     /organism="Prosthecobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_775_length_23611_cov_21.221090"
                     /isolate="AMR_MDS_5629"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1965333"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(208..675)
                     /locus_tag="PLP58_08595"
     CDS             complement(208..675)
                     /locus_tag="PLP58_08595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009963719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HRJ08611.1"
                     /translation="MAHDKRHRTLEGETVNLGFQIAPMIDVVFVIMLFFMVMAGAVKV
                     ERELKTQLPGLGTPAVTNEEVPPDEIMVTVEESNVVTLNEEEFDSPTDKALPNFTGTL
                     MRLKQEADHRGAKVMVTIQAEEQARYERVIDVLNSMAKAQISNVTFTIGGDDF"
     gene            complement(679..1119)
                     /locus_tag="PLP58_08600"
     CDS             complement(679..1119)
                     /locus_tag="PLP58_08600"
                     /inference="COORDINATES: protein motif:HMM:NF014525.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HRJ08612.1"
                     /translation="MAGGGGSTESGEPEFQIAPMIDVLLVLLIFFMSITSAQVLRIDK
                     DIKLPVAADAKKKEDKNAMFEAAINIRWIPTQQKSVIKLDTQELENEEIVNILTERKN
                     NNPVYRAIIRGDRDVPAVEIQKVMALIAQAGIDDISFSALNQDS"
     gene            complement(1162..2037)
                     /locus_tag="PLP58_08605"
     CDS             complement(1162..2037)
                     /locus_tag="PLP58_08605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009963722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HRJ08613.1"
                     /translation="MHTPIQPPMIKNTLSGLVRLSAIALLLFGNLLVVAPVHAQEAPA
                     TEEAVETTQTHTLMDRFHEGGWVMYPLTICSIALVWLTVDLWMRTNVKSMAPPAQVAQ
                     VQDLFRAGDYVGAYQFCKNNNSYFADVARVGLSFSGEGQNATEEALFSELAKVNATVQ
                     TRINYLSVLGVCTPMIGLVGTVTGMMTAFATLGTSGVGDPSKLAGAIGEVLVATASGL
                     AVAVPAFMIFYFLRNRLQGSMHGLQEVVHSLFRKMPYEHLKDAHVGEEEFYAAIPNWV
                     AGGGEPAAAVAVAAD"
     gene            complement(2077..2943)
                     /locus_tag="PLP58_08610"
     CDS             complement(2077..2943)
                     /locus_tag="PLP58_08610"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HRJ08614.1"
                     /translation="MLRHSFILAALILTAQAASAQMLVLKDGTQINHTEFSVQDGKVV
                     RTITIGDQTATTVLPMANIASMDWPEPIEISDARSLLGQGKPDEAVAAIKKAKEFFEV
                     FENIKGTPYREVFFSYVETMSQAGKFEETIKLIPQLRAVQLTDEQKLKLRIIQLDIDR
                     QTSTEYASILAEADSILSDTNDSAIHAAIWMIIADIHLKKKEWEKALMAYMRIPVFYG
                     TQVQRVPDAEMKAAQTLVKMKRYEDAQAVFTRLMDTYKGSPIAQAAEREKAAINGMKN
                     EPEEEPAEEKKS"
     gene            complement(2963..5791)
                     /locus_tag="PLP58_08615"
     CDS             complement(2963..5791)
                     /locus_tag="PLP58_08615"
                     /inference="COORDINATES: protein motif:HMM:NF021492.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HRJ08615.1"
                     /translation="MRHALRLLLLATLVWHPVVQAQDAQPALNIQQETEALMNEGLRL
                     FGEGKYAEALAKIEQVKKNVSGKPFPQVLFVEGASYFNQNEYEKAVVPLEEYVKNFSD
                     GDFINPVRMALGRCYINKGEPDKGIEILKDVVNKAPEMKAEAGLFVAEALKKQEKPDE
                     ALAVLKSVIADGVRSAESIQAAMMAADIHVAKGELDEAGELMEKVKGYATGGDNVAQM
                     NNIYLRLGDQMLEKKSFREALGAYQMVRRKVEISGLQKAVIAKIEQSLANPGKGPIRG
                     TKEELEEKLKVNKGLLEEIETRTDYDASLYYRLGRCYFEMGRFWESLLAFDVIVNEFK
                     EFPQRDRCVFGMIIANAQLKRVKAARELCERYIRDFPDGEDLGTVSEMFGMLAYDAGK
                     LEDAVDSFNKAEGFPKADKERLRFLRGNVLFEMQRFEDARTAFELLVSEFPETAYKDD
                     SLYRIALIYFYQNDSINANKALASYIKENPKGQYVVDARYRMAFIKFQAREVDTAMKE
                     LEKLVEEAPNDQNIGQVHSLLGDAYNQKQDFDKAIEHFALAVDKAKSDDVLTYAMDQA
                     TDLYAGMNKWQELGDMWQKYLATHKDNEDQELKAVLWISRARVKEGKLDEAKKLLSDA
                     IKPKIPNAANQQVEGLIQQLITLSAPKRRRVVVAEGETPPPEITFEQVEKELEEMITP
                     PEAAMNGTAQMRVLFAKAWLAKVMREPDKAEKLFAVIIEVARPDDLSPMLLATVGDNA
                     RRKGDFDKAAGCYNRLNEFFKDTEFADGAAVGLAEIAYEKGENENALELFNKALVDYA
                     GSSRMLDATLGKAKTLFRLKKYDEANKIYAEILNIREWRGEAHAESLFMQGEIEFAQG
                     RFGEAIPFYQRVFIAHQKWKPIMAKAYLQCARAFLQLNRPADPQGMPPRQYADREAAK
                     LTLIEMTKREDLKEFTEYKQAGIELGKL"
     gene            complement(6019..6822)
                     /gene="proC"
                     /locus_tag="PLP58_08620"
     CDS             complement(6019..6822)
                     /gene="proC"
                     /locus_tag="PLP58_08620"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007416296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004735 - pyrroline-5-carboxylate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HRJ08616.1"
                     /translation="MLKIGLVGCGKMGGALLRGVELAFGKGAITAALGDVVPAAVEAL
                     KKSLACKTITGSPGEVAAASEVVLLAVKPDDMKALCQSLAKIKGSRLYLSVAAGVSLA
                     DLEGWLGGRQRVIRSMPNTPALVGKGAAAFAVGTKATHEDAALAARILGGVGIADEVP
                     EKLLDAVTGLSGSGPAYIYTVIEALADGGVLMGLPRASALRLAAQTVAGAAAMVLETG
                     KHPAALRDEVTSPGGTTIAGLEQLENHGLRKALIQAVRAAAGRSRELGG"
     gene            complement(6902..7618)
                     /locus_tag="PLP58_08625"
     CDS             complement(6902..7618)
                     /locus_tag="PLP58_08625"
                     /inference="COORDINATES: protein motif:HMM:NF012976.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="intradiol ring-cleavage dioxygenase"
                     /protein_id="HRJ08617.1"
                     /translation="MNNRAFHIPLNRRLLLRSLLLATGGVITSGVYAEALTLTPRQTE
                     GPYYPDHLPLDQDNDLTMILGGQAPAGGVVTSFGGRLLDADGKPLGGGLIELWQADQN
                     GCYIHSRGAPQGRERDPAFQGYGKITTNEKGEYRFRTIKPGLYTGRTRHFHIAVVKEG
                     KRMLTTQLYLAGEPQNEKDGILRSIRDEMKRLSVIREFKPASGEGGDLTATWDIVIGR
                     TPEDPEPPRRKGTGGPPPRP"
     gene            7729..8472
                     /locus_tag="PLP58_08630"
     CDS             7729..8472
                     /locus_tag="PLP58_08630"
                     /inference="COORDINATES: protein motif:HMM:NF021371.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2071 domain-containing protein"
                     /protein_id="HRJ08618.1"
                     /translation="MPPDHEQREALRAPPARPFVMHQRWGNLLFLHWSFDPKVIQATL
                     PAGLQVDTFEGRAWVGVVPFEMRGIRPRFCPPVPGVSDFLEMNLRTYVHDSNGAPGVW
                     FYSLDCDQPLAVRVARAFFHLPYHHARMSLKISAEGMLRYRSQRRSDDHESAIDYRLG
                     TTVRHARRDTLEYFLAERYLLYALAPSGLHTGRVHHAPYPLVNAEVAEWDTRLFALAG
                     LPEPARAPEHVLGSPGVKVRVWALEKTGL"
     gene            complement(8485..12519)
                     /locus_tag="PLP58_08635"
     CDS             complement(8485..12519)
                     /locus_tag="PLP58_08635"
                     /inference="COORDINATES: protein motif:HMM:NF024777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="plastocyanin/azurin family copper-binding
                     protein"
                     /protein_id="HRJ08619.1"
                     /translation="MPRRLLTLLLSVCSVVQAADLFDQQNLAAWCIVPFDAKKRSPED
                     RAEMVAKMGIKKIAYDWRQEHVVEFEREILAYQKNGIEMFAFWGVHDEALRLFEKYKM
                     HPQLWVMMKGTGDTQEAQVKTAAEGLLPILAKAKAIGSQVGIYNHGGWGGEPENMVAV
                     CEFLKKNHGTDNVGIVYNLHHGHSHLDRLPKALEAMKPWLLCLNLNGMDIAGDTKGRK
                     ILPLGVGTQDVKVLRQIRASGYSGPIGILNHTNEDAEGRLLDNLDGLKWLIPQLDDAP
                     PAAKPKYRTWSEARMKAPSKAPVTVQKASGVPSLNEEFGKALSGGLLLDGKGEYHTLP
                     FTIECRARLDSKERYNILVACNPKSADTHWELYTHAGRGTLALYMPGRGGDYDSKVNI
                     CDGQWHDIVASVSAELATLWVDGKMVLEKPTGKKDGLVIHAGVESIAFGRLVEGTLGC
                     DGVIDDVRLSRGVMKPRKGGAPRQRMDNTLEIWHFDDLGSDAARPKAPDPAPFTPDLP
                     PLNKEDNIHWQEFVNRERVFDFYGKQALQFMKQKPLPDLIPAFPGMDGGKQGHWGNQN
                     DQVTWKDGRFAASDLGTVFSTVFKGAGITIPKGVCVHVKGKGEVATCFDPTTLSFPIV
                     WSGGFVKLNDNRHGFMGGGQMDGKVVETLDSYKKQPPSGRYRGFYRRNDTVVFSYDVN
                     GEEVLDMVWCDGGPERITAPRKKHPFRNYLVLPAQWPQWIETKGELGAQTPFATDRIT
                     IPFENPHGTLFFITAHDFFSDGTAAIATMTGEVWLVKGIDEKLEKLRWKRFATGLHQP
                     LGMKIVKDKLFVLGRDQITCLHDLNGDDEADFYECVTNAMHTSPGGHDFIVGLEADKE
                     GRWYFASGNQGVCRTGILPVTGAAGILPVSAGPGRMPGSHESGGTPDLQVLATGFRNP
                     NGLGISQDGRFITTSVQEGDWTPATSICQIELGKNEGAHFGAGGPKNNQPPEPVLMYL
                     PRGEDNSASSQAFISGEKWASLKGDGNLIHLSSGGGSAWLVMRQNVKGRWQGAAVKIS
                     GNFDSGPQCARFNPKDGHLYVNGMQGWGSYTPKDGCFQRVRFTGGDKPVPVAFEARDN
                     GLLIRFNQAVKDADAATCFAQCWNYKYGPQYGSPEYSVKYADTPGHDPLEVRSVQKLD
                     GGKALFLEIPQIVPANQIHVRLSTGHDVFITAHALAEPFTDFPGYTKIAKTNHAAQIG
                     LEAPKPVKPNPWAKGEPGREIIVEAALGLQFVQKQLMAKAGEKLTIRFNNPDVVPHNW
                     LLAKPGSLQKLGEKCNLMITDPQGLAKHYVPDSDDVIAYTDMVNPKGGFVIHLTAPKE
                     KGDYPYLCTFPGHWMVMNGVMKVE"
     gene            12573..12767
                     /locus_tag="PLP58_08640"
     CDS             12573..12767
                     /locus_tag="PLP58_08640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ08620.1"
                     /translation="MDTTTIDPKLLNDPAFQRVLADALAIQDRFAAEPVYAKTGKGFL
                     KSAGVEELKQIKAAQEQAGK"
     gene            12771..13160
                     /locus_tag="PLP58_08645"
     CDS             12771..13160
                     /locus_tag="PLP58_08645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ08621.1"
                     /translation="MKLSQVWLRTYSWDFITLQNAMLCQAKSALHKPTSDGHAATKEL
                     WESRFQTEMRLDEAIDLCRRCHRMAPFCFYNGNTFAALARDLIQNLALPADEAYIIRS
                     LAGHIVAGVATDEEVRAFREFCERKEA"
     gene            complement(13199..14473)
                     /locus_tag="PLP58_08650"
     CDS             complement(13199..14473)
                     /locus_tag="PLP58_08650"
                     /inference="COORDINATES: protein motif:HMM:NF014081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HRJ08622.1"
                     /translation="MEIQPGTTLITNGQLIDGTGAPPVPNAAVIVTDGLITYAGPAAY
                     APPVPDARKIDAKGGTIMPGLIEAHIHLTYFNVTELQDLDIKFPVEYVTLQSAVNAKT
                     ALECGYTSARSGGCLHNIDVWMKKAIEEDMIPGPRLSASGREICGAGGLMDWNPDYRK
                     IGMEGIVFIVNGPEQARTAVRKLVKDGIEWVKTYPTGDAASPDINDHHTLCMTFEEMH
                     AVVQTSHNHGLKVTGHCRAREGIYNALKAGYDAIEHGTFMDAECLDLLLQRNVPMCPG
                     LGFELWSVERGKDFGLSQRVLDGHQETLEAGAESARKVLKAGGTLGLGGDYGFGWTPH
                     GTYAQELTFFVNYVGFSVLDTIKCATLGGAKIMGREHELGSLEKGKLADVLVVDGDVM
                     KDISILEERNNLLAVMQGGAVKAGTMLQPPSI"
     gene            complement(14486..15253)
                     /locus_tag="PLP58_08655"
     CDS             complement(14486..15253)
                     /locus_tag="PLP58_08655"
                     /inference="COORDINATES: protein motif:HMM:NF020037.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HRJ08623.1"
                     /translation="MKSGAPSEAALCELFDLLPEVQAWIKDARHRYLWVNRAFLLNYG
                     LHQLDEVLGKTDHDLSPPHLAAQFLAGDEAVLAGETVQGRLELIGRFDHTASWSFTTK
                     RQVRDAKGRTIGTTGITRVLDAAQIDQREDIRLGVVISMMTRRLGKAVTNVEMAKAAG
                     MSSRSFERAFLREYGLPAQQYLKRLRIQTACRLLVDTRESIASIAQRCGFADQSHLTR
                     EFRRVTSATPAAYRAEYAAANVPRAAVSVLSTRKRKR"
     gene            complement(15250..16206)
                     /locus_tag="PLP58_08660"
     CDS             complement(15250..16206)
                     /locus_tag="PLP58_08660"
                     /inference="COORDINATES: protein motif:HMM:NF025465.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PmoA family protein"
                     /protein_id="HRJ08624.1"
                     /translation="MKPLSCLPAVIFVLWVPFARSADITAEKTGTVIHFAIEGESICD
                     YQMEPGELPEGVAESFAHGAHLHPIYSPGGKLVTGNHQPDHPWQRGVWIGWTKTEFGD
                     SHPDFWNMGKDGRITAEVRFEKLEKVWSGASGGFMSRHVFLDHSHTPDEKVLNETWEV
                     QVSQKTLDGTLANVIDLTSTQTCAGEKPLKLPKYHYGGLGVRGNPLWNPVDAVTMLTS
                     EGHDRKAGDSTKAKWVHLGGEVDGTPTGIAVLIHPSNFRFPQPLRLNPKNPQLCVAPS
                     QDGDWAIEPGKPHVSKYRLLVFDGKPDAKWLEAQWKSYSAAP"
     gene            16371..22229
                     /locus_tag="PLP58_08665"
     CDS             16371..22229
                     /locus_tag="PLP58_08665"
                     /inference="COORDINATES: protein motif:HMM:NF019323.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-2-macroglobulin"
                     /protein_id="HRJ08625.1"
                     /translation="MSDTSGSWWARLALVMFGRLRYEPPEWLPRCAAKADDHLRRNPG
                     RYAGAIVIAGALAVMVPQILDHLESRRPQPRQLAELRQSKGSLSMPGVTRIVRGKPQP
                     QPLTLRFDASTAPLNAAGKSAAGVTLAPEHEGAWTWGTDKLLVFTPAKDWPAGQEFTV
                     RLDPAELPPEVALKETSWTGRTPALKLEQTVAEFQMDPRDPSLQNVVVGFKSTHPLDR
                     EHITQLTRLEVLGGARIFEWKGRKPDALFSVTEGAHQREFWIRTARIQVPEKEDFLKV
                     TLSKSLTSTLGGAPLGDTFTAKVRVPDIESGFKIAGVETSIIKTEDGEPEQFIFINTE
                     GYATGENMAKHLGVWQEPPNFTVRDALGSRVTPGPNDVTEAVLAQYTPVALKPVETGV
                     DEGQPATTRHAFKFLTEQRGRLFVRVTKGVEGLGGFRLGGEFRVFGAPPSFPKEVQLL
                     GQGGILALHGERKLSLQSRGAPFLRVTMARVPQRQINHLVVMTEGDFATPHFRASWVF
                     DESNLARFLRRVQPVPMKNDYQAAYSTLDFAGALQSADPSDTDASRGLFFLTAEGVKP
                     YTGSQRRMEGDGVAEDWQSLDEDASTRRFVLVTDLGLLVKKNADGSRDVFVQSIQTGD
                     PVDGATITVLAKNGEHLLQAATINGHAHLAATGVFQREKQPVAIVASLGNDVSFLPFE
                     RPDRLLDFSRFDTGGVLASENDALEAFLFTERGVYRPGDDIHIGAVLKRRDWAPGLAG
                     LPLEVDLINARDEVASTQKVVLGADGFVEVKMKTDEAAPTGRHIARLYLIQSGNHRIQ
                     LGQTVLRVEDFQPDRMKIEVALTGNELDGWLQPGSLHADVTLQTLFGFPAAKRLLKAG
                     MELSPGDFAFQQFPGYIFHNRLPVAEDDESLAGQKVGLGEQTTDDEGKARFDLALERF
                     DQGCFQVSLLIEGFEADGGRSVRAGRQFLVAALPYVVGHKPDGGLGYLGKDSDARTRF
                     LAVGPDLKPLAPPDLTARLIHIRHVSVLTQQESGSHAYVSTRRESQLSEAPFTLPAEG
                     ADYTLPTDKAGDFRLELRDAESRVVCAVPFTVVGKGDMEHSLERDTELGIKLARDTWA
                     GGENLEAALSAAYTGAGLLTIERERVLGWQWFKAGTPATVQTIRVPDTLEGSAYVSAS
                     FVRGLDSKEFFASPLSHAVAHFTANPDKRRIQVTLDAPEKVRPGDELKIGFQAARPCR
                     VVIYAVDAGIHQITDYKLPDALAHLNRKRALETETEQLMDLILPEFSHIARHKAFGGD
                     ADKPLRLNLNPFKRRKEPPVVFWSGLIAAGPDRREVGYTVPDYFAGRLNIMAVAVAED
                     AAGRAETSSIARGPLVLTPNAPFFAAPGDEFTASLTVANNLEGAEADAPVRVIAQVTP
                     HLEIIESPQGEVHVPPGREETVRFLMRALEEPGGAEITFKAEAAGQTVLRRATLSVRP
                     ASPYLTHVQSGWFRDGPHDVKVTREMHPHHRKLHASVSALPLGLARGLETYLSEYPYG
                     CSEQITSRAVSRLILSGEADFGFDPAESARQLDTAFALLRSRQHSGGGFGYWDSTSSG
                     GMDYLSTYVTRFLTEARDAGHAVPSALLEGARKHMREMARGTPGSLDDSAVQAAAVYL
                     LTRHGENTSAFVLNLRDHLRQHHDKTWRAHLTAAYLAGAYALLKQQNEGATLMRGYWD
                     AARNDSAEAAWRGGWHLDPQVSRAEGFAVLCRHFPEIAGAFRYQDLEVIIGPLASNRF
                     NTISAATTIMALKAYSDLARESGISVSLSELPRHGGPALLIAPEKTGLIQSPFSAQAG
                     ALRFGLSRQDGADLGAFYQVTESGFDKTPPRERIADGLEVTREILGADGGAVAKLKVG
                     EAALVRLSVRNVSSAALQNIAVLDLMPGSFELEPNGLRPGRGTMPGADYVDVREDRNI
                     FFCSLEKGGARTFAYRVKPIAAGVYAIPPAFAESMYDRSLKGRDGGGSVTVE"
     gene            complement(22259..23263)
                     /locus_tag="PLP58_08670"
     CDS             complement(22259..23263)
                     /locus_tag="PLP58_08670"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021616.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HRJ08626.1"
                     /translation="MPAVSVLMPVRDAEGTVMRAVESIQSQSLRDWELVIVNDGSRDA
                     TPRLLEAARRLDGRLRVLHLPPCGIAPALNAGLAACRAPCVARMDADDLAHPARLELQ
                     LRHMLEQPATGLVSCRVSYQGAGAGYAEHVEWINALGTHEEMSLRRFVEAPVAHPSVM
                     FRRELVDKHGGYRDGPFPEDYELWLRWLEAGARFEKLSESLLVWNDPPQRLSRTDPRY
                     SVAAFYEIKTASLARWLRAQAPEGRALWLWGAGRVTRRRFDALERQGIRLAGFIDVDP
                     AKRGSHRDGRPVRMADELPEREGSFILAGVAARGARGQIHAHLAARGWREGADFLLVA
                     "
     gene            complement(23330..>23611)
                     /locus_tag="PLP58_08675"
     CDS             complement(23330..>23611)
                     /locus_tag="PLP58_08675"
                     /inference="COORDINATES: protein motif:HMM:NF028211.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase C-terminal
                     domain-containing protein"
                     /protein_id="HRJ08627.1"
                     /translation="ALPCSTADEPLLADPGFSALVPPPFSAEAVRHYCEQEWALHLDD
                     VMIRRAGWHYYERDPAALAALAAEIMAGCLGWSPEEKQRELAAYLAHPS"
BASE COUNT         4233 a         7410 c         7360 g         4608 t
ORIGIN      
        1 gcggcatgaa gccgggatgg ggcggaggca gcggctgggt ggctccgggg gcggaaaact
       61 gcgacgcggg atcgccagga ggaggagaag gctgggatgg attggacatg gctggtggag
      121 tctgggttag ccgccggtct tccaatgaag acggcccatt tcagtgacgg gcgtgaggaa
      181 aaaaacttgg gatgaggccg aaatttgtca aaaatcgtct ccaccaatgg tgaaagtgac
      241 gttcgaaatc tgcgccttgg ccatcgagtt aagcacatca atgacgcgct cgtagcgggc
      301 ctgctcctcg gcctgaatgg tgaccatgac cttggctccg cgatgatcgg cctcctgctt
      361 gaggcgcatg agcgtgccgg tgaaattcgg cagcgccttg tcagtcgggg aatcaaactc
      421 ctcctcgttg agagtcacca cgttgctttc ctcgaccgtg accatgatct catcaggcgg
      481 cacctcctca ttggtgaccg ccggtgtgcc aaggcccgga agctgggtct taagctcgcg
      541 ctccaccttg accgcgccgg ccatgaccat gaagaagagc atgatgacga agaccacgtc
      601 aatcatcggg gcgatctgga agccgaggtt gacggtctcc ccctcaaggg tgcggtgtcg
      661 tttgtcgtga gccatgagtc aggagtcctg gttaagggcg gagaaggaaa tatcatcaat
      721 gccagcctgg gctatgaggg ccatgacctt ctggatttcg acggcgggaa cgtcacggtc
      781 gccgcggatg atggcgcgat agacaggatt gttgttttta cgctcggtga gaatgttgac
      841 aatttcctcg ttttccagct cctgtgtgtc gagtttgatg acggacttct gctgggtggg
      901 aatccaacgg atatttatgg cggcctcgaa catggcgttc ttgtcctcct ttttcttggc
      961 gtccgccgcc acaggcagct tgatgtcctt gtcaattctc agcacctgcg ccgaggtgat
     1021 gctcatgaag aaaatgagca gcaccagcag cacatcaatc atcggggcga tttgaaattc
     1081 cggctcgccg ctttccgtgc ttccgcctcc tccagccata tgtgtgtcag gtcaggggtt
     1141 gatgttcggg tttatgcagg ctcaatcagc cgcgacggcg actgcggcgg cgggttcgcc
     1201 accaccggcg acccagttag gaatggccgc gtaaaactcc tcctcgccga catgggcgtc
     1261 cttgagatgc tcatagggca tcttgcggaa gagggaatgc acgacttcct gaagaccgtg
     1321 catggaaccc tggagacggt tgcgcaggaa gtaaaagatc atgaaggcgg gaacggccac
     1381 agccagaccg gaggcggtgg cgacgagcac ctcgccaatg gcgccggcaa gcttcgaggg
     1441 atcgcccaca ccgctggtcc ccagggtggc gaaggcggtc atcatcccag tcacggtgcc
     1501 aaccaatccg atcattggtg tgcagactcc gagaacggac aggtagttaa ttcgggtctg
     1561 gacagtggcg ttgaccttgg ccagttccga gaacaacgcc tcctccgtgg cgttttgacc
     1621 ttcaccggag aagctcaagc ccacgcgggc aacgtcggca aagtatgagt tgttgttttt
     1681 gcagaactgg taggcgccca catagtcacc agcgcggaaa agatcctgga cctgggctac
     1741 ctgggcggga ggggccatgc ttttaacgtt ggtgcgcatc caaagatcca ccgtcaacca
     1801 gacgagagct atggagcaga tcgtaagcgg atacatgacc cagccgccct catgaaagcg
     1861 atccatgaga gtgtgcgtct gcgtcgtctc caccgcctcc tccgtggcgg gcgcctcctg
     1921 ggcgtggacg ggtgctacga cgaggagatt gccaaacagc agaagagcga tggcactgag
     1981 gcggacgagg ccgctgaggg tgtttttgat catgggaggt tggatgggtg tgtgcatgtt
     2041 ggtggtgaat atgaaattga cggagcgctg gaggaatcag cttttctttt cttcagcggg
     2101 ctcctcctcg ggttcgttct tcatgccgtt gatggccgcc ttttccctct ccgcagcctg
     2161 ggcgatggga gagcctttgt aggtgtccat gagccgggtg aaaacggcct gggcatcctc
     2221 gtagcgcttc attttgacaa gtgtctgggc agccttcatt tcggcgtcgg gcacacgctg
     2281 gacctgggtg ccgtaaaaga cagggatgcg catgtaggcc atcagcgcct tttcccactc
     2341 cttctttttg agatggatgt cggcgatgat catccagatg gcggcgtgga tggcggagtc
     2401 attggtgtcg gaaagaatgc tgtccgcctc ggccaggatg gaggcgtact cggtggaggt
     2461 ctgccggtct atgtcgagct ggatgatgcg cagcttgagc ttttgctcgt cggtgagctg
     2521 gacggcgcgg agctggggga tgagcttgat ggtctcctca aacttgccgg cctggctcat
     2581 cgtctccaca taggagaaga agacctcccg gtagggggtg cctttgatgt tttcaaagac
     2641 ttcgaagaat tccttggcct tcttgatggc ggccacggcc tcgtcgggct tgccctggcc
     2701 gaggagggag cgcgcgtcgg agatttcaat cggctcgggc cagtccatgg aggcgatgtt
     2761 ggccatcggc agcacggtgg tggcggtctg gtcgccgatg gtgatggtgc ggacgacctt
     2821 gccatcctgc acgctgaact cggtatggtt gatctgggtg ccgtccttga gcacgagcat
     2881 ctgcgccgag gcggcctggg cggtcagaat gagggcggct aggatgaatg aatggcgaag
     2941 catggcagtg tggaagggcg gttcaaagtt tgcccagctc gatcccggct tgtttgtact
     3001 cggtgaattc cttgaggtcc tcgcgcttgg tcatttcaat gagggtgagc ttggctgcct
     3061 cgcggtccgc atactgacgg ggcggcatgc cctgaggatc ggcgggccgg ttgagctgga
     3121 ggaaggcgcg cgcgcactgg aggtaggctt tggccatgat tggcttccat ttctggtggg
     3181 cgatgaaaac acgctgatag aacgggatgg cctcgccaaa cctgccttgt gcgaactcga
     3241 tctccccctg catgaaaagc gactcggcat gggcctcgcc gcgccattcg cggatgttga
     3301 ggatctccgc gtagattttg ttggcctcat catacttttt gaggcggaac agtgtcttgg
     3361 ccttgccaag cgtggcgtcg agcatccgcg agctgccggc gtaatccacc aaagccttgt
     3421 tgaagagctc cagcgcgttt tcgttctcgc ctttttcata ggcgatttcg gccaggccca
     3481 cggcggctcc atcggcgaac tcggtgtctt tgaaaaactc gttgaggcgg ttgtagcagc
     3541 cggcggcctt gtcgaagtcg cccttgcggc gggcattgtc ccccacagtg gcgagcagca
     3601 tggggctgag gtcgtccggg cgcgccacct cgatgatgac ggcgaaaagt ttctcggcct
     3661 tgtcgggctc gcgcatcacc ttggccagcc aggccttggc aaaaaggacg cgcatctggg
     3721 cggtgccgtt catggctgcc tccggcgggg tgatcatttc ctccagctcc ttctccacct
     3781 gctcgaaggt gatctccggc ggcggtgtct cgccctcggc gacgacgacg cggcggcgct
     3841 tcggagcgct gagcgtgatg agctgctgga tgaggccctc gacctgctgg ttggcggcgt
     3901 tggggatttt cggtttgatg gcgtcggaga ggagtttttt ggcctcgtcc agcttgcctt
     3961 ccttgacacg ggcgcgggaa atccaaagca ccgccttcaa ttcctgatcc tcattgtcct
     4021 tgtgtgtggc gagatatttc tgccacatgt caccgagttc ctgccacttg ttcatgccgg
     4081 cgtagaggtc ggtggcctgg tccatggcat aggtgagcac atcgtccgac ttggccttgt
     4141 ccaccgcgag ggcgaaatgc tcgatggcct tgtcgaaatc ctgcttctgg ttgtaggcgt
     4201 cgccgagcag actgtgaacc tggccgatgt tctggtcgtt cggcgcctct tcgacgagct
     4261 tttccagctc cttcatcgct gtgtccacct cgcgcgcctg gaacttgatg aaggccatgc
     4321 ggtagcgggc gtccaccacg tactggcctt tcggattctc cttgatgtag ctggccaggg
     4381 ctttgttggc gttgatcgag tcgttctgat agaaataaat gagggcgatg cggtagaggg
     4441 agtcgtcctt gtaggcggtt tcggggaact cgctgacgag cagttcaaac gcggtgcgcg
     4501 cgtcctcgaa acgctgcatt tcaaagagca cgttgccgcg cagaaagcgc aggcgctcct
     4561 tgtcggcctt ggggaagccc tcggctttgt tgaaggagtc cacggcgtcc tccagtttgc
     4621 ccgcgtcata ggccagcatg ccgaacatct cggacacggt gccgaggtcc tcgccgtcgg
     4681 ggaagtcgcg gatgtagcgc tcgcacagct cgcgggcggc tttcacgcgc ttgagctgtg
     4741 cgttggcgat gatcatgccg aagacgcagc ggtcgcgctg agggaattcc ttgaactcgt
     4801 tgacgatcac atcaaaggcc agcagggact cccagaagcg ccccatttcg aagtagcagc
     4861 ggcccaggcg gtagtagagg ctggcgtcgt agtcggtgcg ggtttcgatt tcctccagca
     4921 ggcccttatt gaccttcagt ttttcctcaa gctcctcctt ggtgccgcgg atggggcctt
     4981 tgccggggtt ggccaggctt tgctcgatct tggcaatgac ggccttctgc aagcccgaga
     5041 tctccacctt ccgacgcacc atctggtagg cacccagcgc ctcgcggaag ctctttttct
     5101 ccaacatctg gtcgccgagg cggaggtaga tgttgttcat ctgcgcgacg ttgtcgccgc
     5161 cagtggcgta gcctttgact ttctccatga gttcgccggc ctcgtccagc tcgcccttgg
     5221 ccacatggat gtcggcggcc atcatggcgg cctggatgga ctccgcgctg cgcaccccgt
     5281 cggcgatgac ggacttgagc acggcgaggg cctcatccgg cttctcttgt tttttgagcg
     5341 cctcggcgac aaagagtccg gcctcggcct tcatttcggg cgccttgttg accacgtcct
     5401 tgaggatttc gatgcctttg tccggctcgc ctttgttgat gtagcagcgg cccagggcca
     5461 tgcgcacggg gttgatgaaa tccccgtcgc tgaaattttt cacatactcc tccagaggga
     5521 cgacagcctt ttcatactcg ttctggttga aataggaggc accctccaca aagagcacct
     5581 ggggaaaggg cttgccgctg acatttttct tcacctgctc gatcttcgcc agggcctcgg
     5641 catacttgcc ctcaccgaaa agccgcaacc cctcgttcat cagggcctcg gtctcctgct
     5701 ggatgttcag cgcgggctgc gcgtcctggg cctggacgac cgggtgccag acaagcgtgg
     5761 cgagcaataa aagacggaga gcgtgtcgca tgggaaacag aagcggataa aaattgtcca
     5821 gcctgaaaaa aacaatcaaa aaaaacggtg cggccgcgcg cggaattcaa gcgcgtcacg
     5881 gcagaccgga tggtggcgta taaatggcat tttttcgaaa ttgcccgtta aattacaaag
     5941 gctgggattt tttgtcacca acgaactcct tccggcttcg attttcacaa ttccctaccc
     6001 tgcggcctcg ctcgcggatc agccgcccag ttcgcggctt ctcccggcgg ccgcgcgcac
     6061 cgcctggatg agcgctttgc gcaggccgtg attttcgagc tgctccagcc cggcgatggt
     6121 ggtgccgccg gggctggtga cctcgtcacg cagggcggcg gggtgtttgc ccgtctccag
     6181 gaccatcgcc gccgctcccg ccacagtctg ggcggcgagg cgcagggctg acgcgcgcgg
     6241 cagacccatg agcaccccgc catcggccag ggcctcaata acagtatata tataggcggg
     6301 gccgctgccg ctgaggccgg taactgcatc gagcagcttc tccggcacct cgtccgcgat
     6361 gccgacaccg ccgagaatcc tggcggccag agcggcgtcc tcatgagtcg ctttggttcc
     6421 gacggcaaat gcggcggcgc ccttgccgac gagggcgggc gtgttcggca tggagcggat
     6481 gacgcgctgc ctgccgccca gccacccttc cagatccgcg aggctgacac ccgccgcgac
     6541 gctgaggtaa agccggctgc ctttgatctt ggccagggac tggcagagcg ctttcatatc
     6601 atccggtttc acggccagaa gaacgacctc ggacgccgcc gccacttcgc cgggagagcc
     6661 ggtgattgtt ttgcaggcca ggcttttttt caaggcctcg acagcggccg ggaccacatc
     6721 gccaagcgcc gccgtgatgg cgcccttgcc gaacgcaagc tcgacgccgc gcaaaagcgc
     6781 gccgcccatc ttcccgcaac ccacgaggcc aattttcaac atggccgcat gttgggcgcg
     6841 ccgcgccggg acgcaagaag attgaagtcc gtgatgtccc ggaccgccgg atgcgggcgc
     6901 atcaaggccg gggcggtggt ccgccggtgc ctttgcggcg cggcggttcg gggtcctcgg
     6961 gggtgcgccc gatgacaatg tcccaagtgg cggtgaggtc gccaccttcg ccggaggcgg
     7021 gcttgaactc gcgaatcacc gacaggcgct tcatctcatc tcgaatggag cgcagaatgc
     7081 cgtctttttc attctgcggc tcgccagcca aataaagctg ggtggtcagc atccttttgc
     7141 cctccttgac cacggcgatg tgaaagtgcc gcgtgcgccc tgtgtaaagg ccgggcttga
     7201 tggtgcggaa gcgatactcg cccttttcgt tggtggtgat ttttccataa ccctggaagg
     7261 ccgggtcgcg ctccctgccc tgcggtgcgc cgcgactgtg gatgtagcat ccattttggt
     7321 ccgcctgcca aagttcaatg agaccgcctc cgaggggttt cccgtccgcg tcgagcaggc
     7381 gcccgccaaa gctggtgacg acaccgccgg cgggggcctg cccgccgagg atcatcgtca
     7441 aatcgttgtc ctggtccagc ggcaggtgat ccggataata cggcccctct gtctgcctcg
     7501 gtgtaagcgt gagagcctcg gcatagacgc cggaggtgat gacaccgccg gttgcgagca
     7561 ggaggctgcg caaaaggagg cggcggttga gcggaatgtg gaaggcgcgg ttgttcatgg
     7621 ctatgcggct tcaacctctg cgaattagga aagttggaat attgcgaatt ttttttcatg
     7681 tgatgcccaa tcccaggcac agcgggaaca tttcagcgta ttgccttcat gccgcctgat
     7741 cacgaacaac gcgaggctct gcgcgcgccc ccggcgcggc ctttcgtcat gcatcaacgc
     7801 tggggaaatc tcttgttcct gcattggagc ttcgatccaa aggtcatcca ggccacgctg
     7861 cccgcaggac tccaagtgga cacttttgaa gggcgcgcat gggtgggagt ggtgcctttt
     7921 gagatgcgcg gcatccgtcc gcgtttctgc cccccggtgc caggcgtgag cgactttctg
     7981 gaaatgaacc tgcgcaccta tgtccatgac agcaacggcg cgccgggtgt ctggttttac
     8041 tcgctggact gtgatcaacc gctcgctgtg agggtggcgc gcgcgttttt tcacctgccc
     8101 tatcatcatg cccgcatgag tctgaaaata tcggctgagg gcatgctgcg ctaccgttca
     8161 cagcgccgga gtgacgacca cgaatctgcc attgactacc gtctcggaac cacggtgcgg
     8221 catgcgcggc gggacacgct ggagtatttt ctggcggagc gctacctcct gtatgccctt
     8281 gcgccttcgg gtctccatac cggacgcgtt caccacgcgc cgtatccgct ggtcaatgcc
     8341 gaggtggcag aatgggacac acgccttttt gccctggcag gcctgcccga acccgccaga
     8401 gcgccggagc atgtgcttgg atcacccgga gtcaaagtgc gcgtgtgggc gctggaaaaa
     8461 acagggctgt aaaatcgagg ggcatcattc caccttcatc accccgttca tgaccatcca
     8521 gtggccgggg aaggtgcaga ggtagggata atcgcctttt tccttcggtg cggtgaggtg
     8581 aatcacgaag ccgcccttcg ggttcaccat gtccgtgtag gcgatcacgt cgtcgctgtc
     8641 gggcacatag tgtttcgcaa ggccctgcgg gtcggtgatc ataagattgc acttctcgcc
     8701 gagcttttgc agcgaaccgg gcttcgcgag cagccagttg tgcggcacca cgtcggggtt
     8761 attgaagcgg atcgtgagtt tttcgcccgc tttggccata agctgcttct gcacgaattg
     8821 caggcccaaa gctgcttcga cgatgatttc gcgtccgggc tcacctttgg cccacgggtt
     8881 cggtttcacc ggtttggggg cttcgaggcc gatttgcgcc gcgtggttcg ttttggcgat
     8941 cttcgtgtag ccggggaaat ccgtgaacgg ctccgccagc gcatgcgcgg tgatgaaaac
     9001 atcgtgtccg gtgctcagtc ggacgtgaat ctggttcgcg ggcacgattt gcggaatttc
     9061 gaggaagagc gccttgccgc catcgagctt ctgcacgctg cgcacctcca gcggatcatg
     9121 gccgggagtg tcggcatact tcaccgaata ctccggcgag ccgtattgcg gcccgtattt
     9181 gtagttccag cactgcgcga agcatgtcgc ggcgtccgcg tccttcaccg cctggttgaa
     9241 acgaatcagc aagccgttgt cgcgagcctc aaacgccacc ggcaccggct tgtcgccgcc
     9301 ggtgaagcgc acacgctgaa agcagccgtc cttcggggtg tagctgcccc agccctgcat
     9361 gccgttcaca tacagatgcc cgtccttcgg attgaatcgc gcgcactgcg ggccggagtc
     9421 gaaattgccg ctgatcttca ccgccgcacc ctgccagcgg cctttgacgt tttgccgcat
     9481 caccagccat gcgctgccac cgccggagga gaggtggatc aaattgccat cgcctttgag
     9541 cgaggcccat ttctcgccgg agatgaatgc ctggctgctg gcgctgttgt cctcgccacg
     9601 cggcagatac atgagcaccg gctccggcgg ctggttgttc ttcggcccgc cggcgccgaa
     9661 gtgagcgcct tcgtttttac cgagttcgat ctggcagatc gaggtcgcgg gcgtccagtc
     9721 gccttcttgg acgctggtcg tgatgaagcg gccatcttga gagatgccga ggccgttcgg
     9781 attgcggaag ccggtggcga ggacttgtag gtcgggcgtc ccgcctgact catgtgaacc
     9841 gggcatcctg cccggtcctg cggagacggg cagaatgcct gccgcacccg tgacaggcag
     9901 gatgcctgtc ctacacacgc cctgattccc cgatgcgaaa taccatcttc cttccttgtc
     9961 cgcctccagc cccacgatga aatcatgccc gcccggcgag gtatgcatcg cattcgtcac
    10021 gcattcgtag aaatccgctt cgtcatcgcc attcagatca tgcaggcagg tgatttgatc
    10081 acgaccgagc acgaagagct tgtccttcac gatcttcatc cccagcggct gatgcagacc
    10141 ggtggcaaag cgcttccagc gcagcttctc cagcttttcg tcaatgcctt tcacgagcca
    10201 cacctcgccc gtcatcgtgg cgatggccgc agtgccatcg ctgaagaaat catgtgcggt
    10261 gatgaagaac agcgtgccat gcggattctc gaacgggatc gtgatccgat ccgtcgcgaa
    10321 cggtgtttgc gcgccgagtt cacccttcgt ttcgatccac tgcggccatt gggcgggaag
    10381 cacaaggtaa tttcggaagg gatgcttctt gcgaggagct gtaattcgtt ctgggcctcc
    10441 atcgcaccac accatgtcca aaacttcttc tccgttcacg tcatacgaga aaacaacagt
    10501 gtcattcctc cgataaaaac ctctataccg accgctggga ggttgttttt tgtagctatc
    10561 aagcgtctcg accacttttc catccatctg tcctccgccc atgaagccat gtcgattgtc
    10621 atttaatttc acaaagcctc cgctccaaac gattgggaaa gagagtgttg ttggatcaaa
    10681 acatgtggcg acctcacctt tgcctttaac atgcacacaa actccttttg gaattgtgat
    10741 gcccgcacct ttaaacacgg ttgaaaacac agtgccgaga tcgctagctg caaagcgtcc
    10801 atccttccac gtcacctggt cgttttgatt gccccagtgg ccctgcttgc cgccgtccat
    10861 gccagggaag gcgggaatga ggtccggcag cggcttctgc ttcatgaatt gcagcgcctg
    10921 cttgccgtag aagtcgaaga cacgctcgcg attcacgaac tcctgccagt ggatgttgtc
    10981 ctccttgttc agaggcggga gatcaggtgt gaagggagcg ggatcaggcg ccttgggccg
    11041 cgcagcgtcg gagccgaggt cgtcgaagtg ccagatctca agtgtgttgt ccatccgctg
    11101 gcgcggcgcg ccgcctttgc gcggcttcat gactccgcgt gagagacgca cgtcatcaat
    11161 gacgccatca cagccaagcg tgccctcgac gaggcgtcca aaagcgatgg actcgacacc
    11221 ggcgtgaatg acgaggccgt cttttttgcc ggtgggcttt tcgagcacca ttttgccgtc
    11281 cacccagagc gtggcgagct ccgcgctgac gctggccacg atgtcgtgcc attgaccgtc
    11341 gcagatgttc accttcgaat cgtagtctcc gccgcgtccg ggcatgtaga gcgccagcgt
    11401 gccgcgaccg gcgtgcgtgt agagctccca atgcgtgtcg gcggacttgg gattgcaggc
    11461 gacgaggatg ttgtagcgct ccttgctgtc gagcctggcg cggcactcga tggtaaaggg
    11521 cagcgtgtgg tattcgcctt tgccatccaa cagcaggccg ccggagagcg ctttgccgaa
    11581 ttcttcattc aaagacggga cgccggaggc tttctggacg gtgacgggag ctttggaggg
    11641 cgctttcatc ctggcctcgc tccaggtgcg atacttcggc ttcgcggcag gcggtgcgtc
    11701 atcgagttgt ggaatgagcc acttcaagcc gtcgaggttg tcgagcaggc ggccttcggc
    11761 gtcttcgttc gtgtgattca ggatgccaat cggcccgctg tagccgctgg cgcggatctg
    11821 gcgcagcacc ttcacatcct gcgtgccgac gccgagcggg aggatcttgc gacccttcgt
    11881 gtcgcccgcg atgtccatgc cgttgaggtt gaggcaaagc agccagggct tcatcgcttc
    11941 gagcgccttt ggcaggcggt cgagatggct gtgaccgtga tgcaggttgt aaacaatgcc
    12001 cacgttgtcg gtgccgtggt tcttcttcaa aaactcgcac accgcgacca tgttctccgg
    12061 ctcgccgccc cagccgccgt ggttgtagat gcccacctga ctgccgatgg ccttcgcctt
    12121 ggccaaaatg ggcagcagac cttcagccgc agttttgacc tgggcttcct gcgtatcgcc
    12181 cgtgcccttc atcatcaccc aaagctgcgg atgcatcttg tacttctcaa aaagccgcaa
    12241 cgcctcgtcg tgcacccccc agaacgcgaa catctcgatg ccattcttct gatacgcgag
    12301 gatctcgcgc tcaaactcca ccacatgctc ctgccgccaa tcgtaggcga tcttttttat
    12361 ccccatcttc gccaccatct cggcgcggtc ttccggtgag cgcttcttcg catcaaacgg
    12421 cacaatgcac cacgcggcga ggttttgttg gtcgaagagg tccgcagcct ggaccacgga
    12481 acacacagaa agcagaaggg tgagaagtcg gcggggcatg gcgggcgaat tcggggattg
    12541 cgaagaacgg cggcggaaga taaactcagg acatggatac gacgacgatt gatccgaaac
    12601 tactgaacga tcccgccttt cagcgcgtgc tggcggatgc gctggcgatt caggatcgtt
    12661 tcgccgccga gccggtttat gccaagacag gcaaaggttt tctcaagagc gcgggcgtgg
    12721 aggaattgaa gcagatcaaa gcagcgcagg aacaagccgg aaagtgattc atgaagctct
    12781 cccaagtttg gttgcgaacc tattcgtggg atttcatcac actgcaaaac gcgatgctgt
    12841 gccaggcgaa gtccgcgctg cacaaaccca cctcggacgg tcatgcggcg acgaaagaac
    12901 tttgggaaag ccgtttccag acggagatgc gtctggatga agccatcgac ctttgccgtc
    12961 ggtgccaccg aatggctccg ttctgctttt acaacggcaa cacttttgcc gcgttggctc
    13021 gtgatctgat ccaaaacctc gcccttcccg ccgacgaagc ctacatcatc cgcagtctcg
    13081 ccggccacat cgtcgcaggt gtcgccacgg acgaggaagt gcgggcgttt cgcgagtttt
    13141 gcgaacggaa ggaggcttaa aagccttcga tccgcatgaa gcattggcat ggcaggcatc
    13201 agatggaggg cggttgcagc attgtccccg ccttcaccgc gccgccttgc atcacggcga
    13261 gcaggttgtt gcgctcttca aggatgctga tgtccttcat cacgtcgcca tcgacgacta
    13321 gcacgtcggc gagcttgcct ttttcgagtg aaccgagttc atgctcgcga cccatgatct
    13381 tcgcgccgcc gagcgtggcg cacttgatcg tgtcgaggac gctaaagccg acgtagttca
    13441 cgaagaaggt cagctcctgc gcgtaagtgc cgtgcggcgt ccagccaaag ccgtaatcgc
    13501 caccgaggcc gagcgtgccg ccggccttga gcactttgcg ggcgctctcg gctccggctt
    13561 cgagggtttc ttgatggccg tcgagcacac gctgcgacaa accgaagtcc ttgccgcgct
    13621 cgacgctcca cagctcaaac ccaagcccag gacacatcgg cacgttgcgc tgcaaaagca
    13681 gatcgagaca ctcggcgtcc atgaaggtgc catgctcgat ggcgtcgtaa cctgccttga
    13741 gcgcattgta gatgccttca cgagcgcggc agtgtccggt gactttgagt ccgtgattgt
    13801 gcgaggtctg cacgaccgca tgcatttcct cgaaggtcat gcacagcgtg tgatggtcgt
    13861 tgatgtctgg cgaggccgcg tcgcccgtgg gataggtttt cacccactcg atgccgtctt
    13921 tgacaagctt gcgcacggcg gtgcgtgcct gctccgggcc gttcacgatg aagacaatgc
    13981 cctccatgcc gattttccgg tagtccgggt tccagtccat caaacctccc gcgccacaga
    14041 tttcacggcc gcttgctgaa agacgaggac cagggatcat gtcctcctcg atggccttct
    14101 tcatccacac gtcgatgttg tgcagacatc cgccgctgcg agcactggtg tagccgcatt
    14161 cgagcgccgt cttcgcattc acggccgatt gcagcgtgac gtattcgacc gggaacttga
    14221 tgtcgaggtc ctgcagctcg gtgacattga aataggtcag gtgaatgtgc gcctcgatca
    14281 gccccggcat gatcgtgccg cccttcgcat cgatcttccg cgcgtccggc acgggcggag
    14341 cgtatgcagc aggtcctgcg taagtgatga gtccatcggt gacgatgacg gcggcattcg
    14401 gcacgggagg cgcgccggtg ccgtcgatga gttgaccgtt ggtgatgaga gtagtgcctg
    14461 gttggatctc catggtgctc cgaggctagc gtttgcgctt cctggtggat agaacagaaa
    14521 cggcagctct tggaacattc gcggcggcgt attcggcgcg ataagccgcg ggcgtggcac
    14581 tggtgacgcg gcgaaattca cgagtgagat ggctttgatc cgcgaacccg cagcgctgcg
    14641 cgatggaggc aatcgactca cgcgtgtcca ccagcagccg acaggccgtc tggatgcgca
    14701 gacgcttcaa atactgctgc gctggcaggc cgtattcacg aaggaaggcc cgctcaaagg
    14761 accgcgatga cattccggca gcttttgcca tctccacatt cgtcaccgcc ttgccaagcc
    14821 tccgagtcat catggaaatg accacgccga ggcggatgtc ctcacgctga tcgatctgcg
    14881 cggcatccag cacgcgcgtg atgcccgtgg tgccgatggt gcgtcctttc gcatcacgca
    14941 cctggcgttt cgtggtgaag ctccacgaag cggtgtggtc aaaacggcca atcaactcca
    15001 gccggccctg caccgtctct ccggccagca ccgcctcgtc tcccgcaaga aactgagccg
    15061 caagatgcgg cggcgacaga tcatgatccg tcttccccaa aacctcgtcg agctgatgca
    15121 gtccgtagtt gagcaaaaag gcgcgattca cccacaggta gcgatgtcgc gcatccttga
    15181 tccatgcctg cacctccggc agcagatcaa agagctcgca aagcgcggcc tcggatggag
    15241 cgccggattt catggcgcag ccgaatagct cttccactgc gcttcgagcc acttcgcgtc
    15301 cggtttgccg tcgaaaacga gaaggcggta tttggacaca tgcggcttcc ccggctcgat
    15361 ggcccagtcg ccatcctgag aaggggccac gcagagctgc gggttcttcg ggttcaaacg
    15421 cagcggctgc ggaaagcgaa aattggacgg atgaatgagc acggcgatgc cggtgggcgt
    15481 gccgtcaacc tcaccgccga ggtgaaccca cttcgccttg gtgctgtcgc ccgctttgcg
    15541 gtcatggcct tcgctggtga gcatggtgac ggcatccacg ggattccaga gcgggttccc
    15601 gcgcacgccg aggccgccgt agtggtattt cggcagcttg aggggcttct cacccgcgca
    15661 ggtctgcgtg ctggtgaggt cgatcacatt cgcgagcgtg ccatcaagcg tcttttgcga
    15721 tacctgcacc tcccaggtct cattcagcac cttttcatcg ggcgtgtggc tgtgatcgag
    15781 aaacacatgc cggctcatga aaccgcccga ggccccgctc cagaccttct ccagcttctc
    15841 gaagcgcact tcggcggtga tcctgccatc cttgcccatg ttccagaaat cggggtgcga
    15901 atcgccaaac tccgtcttcg tccagccgat ccacacaccg cgctgccacg gatgatccgg
    15961 ctgatggttg cccgtcacga gtttcccgcc cggcgagtag atcggatgca ggtgcgcccc
    16021 gtgcgcaaac gactccgcca cgccctccgg cagttcaccg ggctccattt gataatcaca
    16081 gatgctttcc ccctcgatgg cgaagtggat gacggtgccg gttttctcgg cggtgatgtc
    16141 cgcggagcgg gcaaaaggca cccaaaggac gaagatgacg gcagggaggc atgaaagcgg
    16201 tttcatgacg atgaaacgga caggggactg gcaaactcgc aagcttcgcg ctccgcacgg
    16261 gcgctcccgg atccgcgacc gacagggcgc aaaaagttcc atcctggcga cattttcccc
    16321 gcgcagccat gcgcgccgga ggtattctgg agcaccgacc tttttctttt atgtccgata
    16381 cctcaggctc ctggtgggcg cgtctggcgc ttgtgatgtt tggccgcttg cgctacgagc
    16441 cgccggagtg gctgccgcgc tgcgcggcaa aggcggacga tcatctgcgg agaaacccgg
    16501 gccgttacgc cggagccatc gtcatcgccg gcgcgctggc tgtcatggtg ccgcagattt
    16561 tggaccacct cgaatcacgc cgtccgcagc cgaggcagct cgccgaactg cgccagagca
    16621 agggctcgct gagcatgccc ggcgtgacac gcatcgtcag gggcaagccg caaccgcagc
    16681 cgctgacact gcgtttcgac gcctccaccg ctccgctcaa tgccgctggc aaatccgccg
    16741 cgggcgtgac actggcgccg gagcatgagg gcgcgtggac atggggcacg gacaagctgc
    16801 tggtcttcac ccccgccaag gactggcccg ccggccagga gttcaccgtg cggctcgacc
    16861 ccgcggaact gccgccggag gtggcgctca aagagacctc ctggacgggc cgcacgccgg
    16921 ccttgaagct ggagcagacc gtggccgagt tccagatgga cccgcgcgac ccctccttgc
    16981 aaaacgtcgt cgttggcttc aaaagcacgc atcccctgga cagggagcac atcacccaac
    17041 tgaccaggct ggaggtgctg ggcggggcgc gcatttttga atggaaaggc cgcaagccgg
    17101 acgcgctctt cagcgtgacc gaaggcgcgc atcagcggga gttctggatc cgcaccgcgc
    17161 gcatccaggt gccggagaag gaggattttc tcaaagtcac gctctccaag tccctgacca
    17221 gcacgctcgg cggcgcgccg ctgggggaca ccttcaccgc caaggtgcgc gtgccggaca
    17281 ttgagagcgg cttcaaaatc gccggcgtgg agaccagcat tatcaagacc gaggacggcg
    17341 agccggagca gttcattttt atcaataccg aaggctacgc caccggcgaa aacatggcca
    17401 aacatctcgg cgtctggcag gagccgccca acttcactgt gcgcgacgcc ctgggaagca
    17461 gagtgactcc cggccccaat gatgtgacgg aggccgtgct ggcgcaatac acgccggtgg
    17521 cgctcaaacc cgtggagacc ggggtggacg aagggcagcc ggccacgaca cgccacgcct
    17581 tcaagttcct caccgagcag cgcggcagac tttttgtccg tgtcaccaag ggcgtcgagg
    17641 gcctgggcgg cttccggctt ggcggcgagt tccgtgtctt tggcgctccg ccatccttcc
    17701 cgaaggaggt gcagctcctc ggccagggcg ggattctggc cctgcatggc gagcgcaaac
    17761 tctccctgca atcccgcggc gcgccctttc tgcgcgtgac catggcgcgc gtgccgcagc
    17821 gccagatcaa tcatctcgtg gtcatgacgg agggggactt tgccaccccc cacttccgcg
    17881 caagctgggt cttcgacgag tcaaacctcg cccgcttcct gcgccgcgtg cagccggtgc
    17941 cgatgaaaaa cgactaccag gccgcctact ccacgcttga cttcgccggc gcgctccagt
    18001 ccgcggaccc atcggacacc gacgcctcgc gcgggttgtt cttcctgacg gcggaaggcg
    18061 tgaagcccta caccggcagc cagcggcgca tggagggcga tggcgtggcc gaggactggc
    18121 aatcgctgga tgaagacgca agcacgcgcc gcttcgtgct ggtgacggac ctcggactgc
    18181 tggtgaaaaa gaacgccgat ggcagccgcg atgtctttgt gcagagcatc cagaccggcg
    18241 accctgtgga cggcgccacc atcaccgtgc tggcaaaaaa cggcgagcac ctgctgcagg
    18301 ccgccaccat caacggccac gcgcacctcg cagccaccgg cgtctttcag cgcgagaagc
    18361 agcccgtggc catcgtcgcc tcccttggca atgacgtgtc cttcctgccc ttcgagcgcc
    18421 ccgaccggct cctggacttc tcccgcttcg acaccggcgg agtgctggcc tccgaaaacg
    18481 acgcgctgga ggcctttctc ttcaccgagc gcggcgttta ccgtcccggt gacgacatcc
    18541 acatcggcgc ggtgctcaag cgccgggact gggcgccggg actggccgga ctgcccctgg
    18601 aggtggactt gatcaacgcc agggacgaag tcgcctccac ccagaaagtc gtgctcggcg
    18661 cggacggctt cgtggaggtg aaaatgaaga ccgacgaggc cgcccccacc ggacgccaca
    18721 ttgcccggct gtatttgatc cagagtggaa accaccgcat tcaactcggc cagaccgtgc
    18781 tgcgcgtgga ggacttccag ccggaccgca tgaagatcga ggtggcgctc accggcaacg
    18841 aactcgacgg ctggctgcag cccggaagcc tccacgcgga tgtcaccctg caaactttgt
    18901 tcggtttccc ggcggcgaag aggctgctga aggcgggcat ggagttgagt cccggagact
    18961 tcgccttcca acaattcccc ggctacatct tccacaaccg cctgcccgtg gcggaggatg
    19021 acgagtccct cgcaggccaa aaagtcggcc tcggcgagca gaccaccgat gacgagggaa
    19081 aggctcgctt tgacctggcg ctggagcgtt ttgaccaggg ctgcttccag gtcagcctgc
    19141 tcatcgaggg cttcgaggcc gacggcgggc gcagcgtgcg cgcgggcagg cagttcctgg
    19201 tggccgctct gccctatgtg gtcggccaca agccggacgg cggtcttggt tatctcggca
    19261 aggactcgga cgcacgcacc cgcttcctgg cagtggggcc ggatttgaag ccgctggcgc
    19321 cgccggacct caccgcgcgc ctcatccata tccggcatgt gtccgtgctc acccagcagg
    19381 agagcggcag ccatgcctac gtctccaccc ggcgcgagtc ccagctttcc gaggcgccct
    19441 tcaccctgcc ggctgagggc gcggactaca cactgccaac ggacaaagcg ggcgacttcc
    19501 gcctggagct gcgcgacgcg gagagccgcg tcgtctgcgc cgtgcccttc actgtcgtgg
    19561 gcaaaggcga catggagcac agcctggagc gcgacaccga gctgggcatc aagctcgcgc
    19621 gcgacacctg ggccggcggc gagaacctgg aggccgccct cagcgccgcc tacaccggcg
    19681 cgggtctgct caccatcgag cgcgagcgcg tgctcggctg gcagtggttc aaagcgggca
    19741 ctccagccac tgtgcaaacc atccgcgtgc ctgacacgct tgagggttcc gcttatgtca
    19801 gcgcatcctt cgtgcgcgga ctcgactcga aggaattctt cgcctccccg ctcagccatg
    19861 ccgtcgcgca tttcaccgcc aatccggaca aacggcgcat ccaggtcaca ctcgacgcgc
    19921 cggaaaaagt ccgccccggt gacgagctca agatcggctt ccaggccgcg cgcccctgcc
    19981 gcgtggtcat ctatgcggtg gacgcgggca tccaccagat caccgactac aagctgcccg
    20041 acgcgctcgc gcacctgaac cgcaaacgcg ccctcgaaac agagaccgag cagctcatgg
    20101 acctcatcct gcccgagttt tcccacatcg cccgccacaa ggccttcggc ggtgatgccg
    20161 acaaacccct gcgcctgaac ctcaacccct tcaaacgccg caaggagccg cccgtggtct
    20221 tctggtccgg cctcatcgcc gccggacccg atcggcggga ggtcggctac accgtgccgg
    20281 attacttcgc gggcaggctc aacatcatgg ccgtggccgt ggcggaggat gctgcgggcc
    20341 gcgccgagac ctccagcatc gcgcgcgggc cgctggtgct gactcccaac gcgcccttct
    20401 tcgccgcgcc gggggacgag ttcaccgcct ccctcaccgt ggcgaacaac ctcgaaggcg
    20461 cggaagcgga cgcgcccgtg cgcgtcatcg cgcaggtgac tccgcatctg gagatcatcg
    20521 agtctccgca gggggaagtg catgtgccgc cgggtcgtga ggaaaccgtg cgcttcctca
    20581 tgcgcgcgct ggaggagccg ggcggcgccg agatcacctt caaagccgag gccgccgggc
    20641 agactgtgct gcgccgcgcc accctcagcg tcaggcccgc gtccccctac ctcacgcatg
    20701 tgcaaagcgg ctggttccgg gacgggccgc atgacgtcaa agtcacgcgc gagatgcatc
    20761 cgcatcatcg caaactccat gccagcgtct ccgccctgcc gctcggactc gcgcgagggc
    20821 ttgagaccta tttgagcgag tacccctacg gctgctccga gcagatcacc agccgcgctg
    20881 tgtcccgcct catactttcg ggcgaggccg acttcggctt cgaccccgcc gagtccgccc
    20941 gccagctcga caccgccttt gccctgctgc gcagccgcca gcactccggc ggcggctttg
    21001 gctactggga ctccacatcc agcggtggca tggactacct ctccacctac gtcacccggt
    21061 tcctcaccga ggcgcgggac gccggccacg ccgtgccctc cgcgctgctg gaaggcgcgc
    21121 gcaagcacat gcgcgaaatg gcgcgcggca ctccaggcag cctggatgac tcggcggtgc
    21181 aggcagcggc cgtttacctc ctgacccggc acggcgaaaa cacctccgcc tttgtcctca
    21241 acctgcgcga ccacctgcgc cagcaccacg acaagacctg gcgcgcccac ctcaccgccg
    21301 cctacctggc cggcgcctac gccctgctca agcagcaaaa cgagggcgcc accctcatgc
    21361 gcggctactg ggacgccgcg cgcaatgaca gcgcggaggc tgcctggcgc ggcgggtggc
    21421 acctcgaccc gcaagtgagc cgcgcggagg gcttcgccgt gctgtgcagg cacttcccgg
    21481 agatcgccgg cgcgtttcgc taccaggatc tggaggtcat catcggaccg ctcgccagca
    21541 accgcttcaa caccatcagc gccgccacaa ccatcatggc tctcaaagcc tactccgacc
    21601 tggcgcgcga gtccggcatc agcgtctccc tcagcgagct gccgcgccat ggcggccccg
    21661 cgctcctcat cgcgccggaa aagaccggcc tcattcaaag ccccttcagc gcgcaggccg
    21721 gggcgctgcg cttcggcttg tcacggcagg acggcgccga cctcggcgcc ttttatcaag
    21781 tcacggagtc cggcttcgac aaaacaccgc cccgcgagcg catcgcggac ggtctggagg
    21841 tgactcggga aatcctcggc gcggacggcg gcgctgtggc aaaactcaaa gtcggcgagg
    21901 ccgccctcgt gcgcctgagc gtgcgcaacg tctcatccgc cgcgctgcaa aacatcgccg
    21961 tcctggacct gatgcccggc agcttcgagc tggagcccaa cggcctgcga ccggggcgcg
    22021 gcaccatgcc cggcgcggat tatgtggatg tgcgcgagga ccggaacatc ttcttttgca
    22081 gcctggaaaa gggcggggcg cgcaccttcg cctaccgcgt caaacccatc gccgccggtg
    22141 tctatgccat cccgcccgcc ttcgccgaaa gcatgtacga ccgctccctc aaaggccgcg
    22201 acggcggcgg cagcgtgacc gtggagtaac ccacgcccgg acgatgccgc cgcggcgctc
    22261 aagccaccag aaggaaatcc gcgccctccc gccagccgcg cgccgcgagg tgggcgtgga
    22321 tttgtccgcg cgcgccgcgc gcggcgactc ccgcgaggat gaagctcccc tccctctccg
    22381 gcagttcgtc cgccatgcgc acggggcgtc cgtcgcggtg gctgccgcgc ttggcggggt
    22441 ccacgtctat gaaacctgcg aggcggatgc cttgacgctc cagcgcgtcg aagcgcctgc
    22501 gtgtgacacg gcccgcgccc caaagccaca gagcgcgccc ctccggtgct tgggcgcgca
    22561 gccagcgggc gaggctggcg gttttgatct cataaaacgc ggccaccgag tagcgcggat
    22621 cagttcgcga gagccgctgc ggcgggtcat tccagacgag caaagactcg ctcagcttct
    22681 caaagcgcgc gccagcctcc agccagcgca gccaaagctc gtaatcctcg gggaacggcc
    22741 cgtcgcggta acctccgtgc ttgtccacca gctcgcggcg gaacatgacg ctgggatgcg
    22801 ccacaggcgc ctcgacaaag cggcgcagac tcatctcctc atgcgtgccg agtgcgttga
    22861 tccattccac atgctccgcg tatcctgccc cggcgccctg gtagctcacc cggcatgaga
    22921 caagaccggt ggcgggctgc tccagcatgt gccggagctg gagttcgagc cgcgcggggt
    22981 gcgccaggtc atcggcgtcc atgcgcgcga cgcacggcgc gcggcacgcg gcgagtccgg
    23041 cgttcaatgc cggcgctatg ccgcatggag gcagatgcag cacgcgcaac cgcccgtcca
    23101 gacggcgggc ggcttccagc agacgcgggg tggcatcgcg cgagccgtcg ttgacgatga
    23161 cgagctccca gtcgcgcagg ctctggcttt gaatgctctc cacagcacgc atcacggtgc
    23221 cttcggcatc gcgcacgggc atgaggacgg agacggcggg catggttttg aagaaagtcg
    23281 gccttcactg ggacggcgcc ccagcgcgca agcggctggc gcgcgctttt catgacggat
    23341 gggccaggta ggcagccagc tcgcgctgtt tttcctccgg tgaccagcca aggcagccag
    23401 ccatgatctc ggcggccagg gcggcgagtg cggcgggatc gcgctcgtaa taatgccagc
    23461 cggcgcggcg gatcatcacg tcatcaagat gcagcgccca ctcctgctcg cagtaatggc
    23521 gcacagcctc ggcgctgaag ggtggcggca caagtgcgct gaaaccgggg tccgccagca
    23581 gcggctcatc ggcggtggag cagggcaggg c
//
LOCUS       DAOMYJ010000063        23496 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Prosthecobacter sp. isolate AMR_MDS_5629
            NODE_781_length_23496_cov_22.114884, whole genome shotgun sequence.
ACCESSION   DAOMYJ010000063 DAOMYJ010000000
VERSION     DAOMYJ010000063.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867850
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prosthecobacter sp. (wastewater metagenome)
  ORGANISM  Prosthecobacter sp.
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Verrucomicrobiaceae; Prosthecobacter.
REFERENCE   1  (bases 1 to 23496)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 23496)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.30
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:34:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,742
            CDSs (total)                      :: 4,684
            Genes (coding)                    :: 4,676
            CDSs (with protein)               :: 4,676
            Genes (RNA)                       :: 58
            tRNAs                             :: 55
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23496
                     /organism="Prosthecobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_781_length_23496_cov_22.114884"
                     /isolate="AMR_MDS_5629"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1965333"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            114..269
                     /locus_tag="PLP58_08680"
     CDS             114..269
                     /locus_tag="PLP58_08680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ08628.1"
                     /translation="MEVFAVTIFMSLAFAVLFAVLFLAERGQRQRRSVEQMSLLPLEA
                     DQDHAGR"
     gene            291..2675
                     /gene="ccoN"
                     /locus_tag="PLP58_08685"
     CDS             291..2675
                     /gene="ccoN"
                     /locus_tag="PLP58_08685"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009958922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome-c oxidase, cbb3-type subunit I"
                     /protein_id="HRJ08629.1"
                     /translation="MTSASTAKTLIEFDDRTVRRFMIASIIWGVVGMLAGVFIALQLN
                     FHELNLASWLSFGRLRPLHTNAVIFAFVGNMMFAGIYYSTQRLCKARMASDLLSNIHF
                     WGWQLIIVSAAVTLPLGLTRGQEYAELIWPVNIAVALIWVVFAVNFFWTLAKRNEPSL
                     YVALWFYIATIITVAMLYIVNHISIPTSWTHSYTIFAGVQNGLVQWWYGHNAVAFFLT
                     TPILGIMYYFLPKAVERPVYSYRLSIVHFWSLVFIYIWAGPHHLLNTALPKWLQMLGM
                     FFSLMLWAPSWGGMLNGLLTLRGAWDKLRTDPVVKFYIAAVTFYGMSTFEGPLLSIRA
                     VNALSHYSDWTIGHVHSGALGWNGLMAAGMFYWLTPKLYGGKLHSVAWANFHFWIATI
                     GILIYVAAMWVSGIMQGLMLNATNAEGTALVYPNFIETLTSIRAMMGFRIIGGALYLA
                     GFILMGVNLWLTIRKGKPVNETREVAVIQPKAKDTMGMRDTFINDPVAYMFAGMVLVL
                     AWLFLPPGADIAALLCAGVFSVLAVKKFREGHSRWSSWYEGLLNNWLPFTVLTFVAVA
                     LGGLIQIIPTVTVNRAKNVEDRIQQAYTPLELAGRDIYVSEGCYNCHSQMIRTMLPDV
                     LRYGDYSRLGESIYDHPFQWGSKRTGPDLARIGDKYPHSWHYDHMMEPRSTSVGSNMP
                     AYPHLFEQKFDQKSLPKKIAVMTRLGVPYPPMSADEIKMKALEQAVKIAEELKAQGKV
                     AAPDTQIVALIAYMQKLGKYDTPEVEKKLAPQGVPFPLKPALPDSHRKSVTQNP"
     gene            2688..2843
                     /locus_tag="PLP58_08690"
     CDS             2688..2843
                     /locus_tag="PLP58_08690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ08630.1"
                     /translation="MYKRVLYENWADWVPYLAFGITALVFISFVVRAVTLRKERADQM
                     ARMPLED"
     gene            2863..3462
                     /locus_tag="PLP58_08695"
     CDS             2863..3462
                     /locus_tag="PLP58_08695"
                     /inference="COORDINATES: protein motif:HMM:NF024834.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome c oxidase N-terminal
                     domain-containing protein"
                     /protein_id="HRJ08631.1"
                     /translation="MSSHNDTSDGPILRDHVYDGIQEYDQKLPNWWLFTWYITMVWFV
                     IAWVAYYQLGRGDSDVEQIDKAIAAVEKHRDEELAALDDDKLWEMSKDAKVVEAGKAI
                     YSTTCVACHAPDLSAHIAGVKLPGLPLNDSEWKHGGAPTQILNIIRKGAPDLTKGMPP
                     WEPQLGIQRVVEVTAFLLSHHEKGKPVTLAADSPLKTGG"
     gene            3528..4841
                     /locus_tag="PLP58_08700"
     CDS             3528..4841
                     /locus_tag="PLP58_08700"
                     /inference="COORDINATES: protein motif:HMM:NF015916.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008092 - cytoskeletal protein binding
                     [Evidence IEA];
                     GO_function: GO:0030674 - protein-macromolecule adaptor
                     activity [Evidence IEA];
                     GO_function: GO:0042802 - identical protein binding
                     [Evidence IEA];
                     GO_function: GO:0043130 - ubiquitin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SHD1 domain-containing protein"
                     /protein_id="HRJ08632.1"
                     /translation="MRALHLPRAAARMKPVMAGMFFLAAALPAQDGPRKWTDAQGRTV
                     EAEFAGLDGETVLLKMSGGQTVPYPLAKLSAEDQAFVSKRKEASAPAGPSAAGPRQPL
                     EKREWPGNVAVSTRAIEIKVVEEKAAERRYVYQSEAFEFTSQAKLAGSVMTEVARTFE
                     ATRAVINALPWGVVCQPPDGMPRFLAALYETRQDYVDAGGPELSGGVYQTAEKTFRIP
                     FQSLGLEKRGQTYFKNDFSNDTLVHEITHQMMHDYLGFLPTWLIEGMAEYVELLPYRA
                     GVFRADAHKTAMKSTVDEWIKSGRPPMIPSLEAHMSMNREGWGAETAEAEKMREMYRR
                     SILLVYYFSHLDGDRTGARFLRFMEAVYGEVEAMRAFFADPRVKRMEGGRFTYPSSLK
                     PPGMYKDETIFRHLGILVDERPYEKIAADIVEGYKTIGVKVDVRP"
     gene            4963..5673
                     /locus_tag="PLP58_08705"
     CDS             4963..5673
                     /locus_tag="PLP58_08705"
                     /inference="COORDINATES: protein motif:HMM:TIGR02046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase cytochrome b subunit"
                     /protein_id="HRJ08633.1"
                     /translation="MSAVFDSISRFYSSSIGKKILVALTGAALALFVLGHMIGNLLVF
                     AGPDAINEYGHMLQTALHGGGVWIARIGLLAAVAVHVVATVSLTKANKAARPDAYGQH
                     KAQVSGRSSRIMIWSGLTVLAFIIFHLLHFTIRAGNDFNGAAYKTELHGETVHNVFKM
                     VIDGFSSTPASIFYLIAMALLCSHLSHGFSSLFQTLGISTDKTEPLFKKAGYAFAGLI
                     FAGNAAIVLSIWLFGYGR"
     gene            5719..7635
                     /locus_tag="PLP58_08710"
     CDS             5719..7635
                     /locus_tag="PLP58_08710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007418039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase/succinate dehydrogenase
                     flavoprotein subunit"
                     /protein_id="HRJ08634.1"
                     /translation="MSLDSHIPSGPMAMKWSKHKQDSKLINPKNKRKFTVLVVGSGLA
                     GSSAAATLSELGYKVKCFCFQDSPRRAHSIAAQGGINAAKNYQNDGDSVHRLFYDTVK
                     GGDFRAREANVHRLAEVSVNIIDQCVAQGVPFAREYGGMLANRSFGGAQVSRTFYARG
                     QTGQQLLLGAYSALNKEIAAGGVQMYPRTEMLDVVIVDGHAKGIITRNLVTGKIEAHA
                     GDAVLLCTGGYGNVFYLSTNAMGCNVTATWRAHRKGAFFANPCYTQIHPTCIPVSGDY
                     QSKLTLMSESLRNDGRVWVPKKKEDCGKAPSQIAEADRDYYLERKYPSFGNLAPRDIS
                     SRAAKEACDDGRGVGPGGRGVYLDFAEAIGHFGAKTIAERYGNLFEMYERITGESAYK
                     QPMRIYPAVHYTMGGLWVDYNLMSNLPGLHVLGEANFSDHGANRLGASALMQGLADGY
                     FVIPTTIANYLVHQKPGSVTAEHPEFKKALEVCTAQTKGFLNSKGKRSVDSFHRELGL
                     LVWDKCGMARTKEGLTEALKRIPELREEFKTNLLVPGSGDACNPELEKAGRVADFMEF
                     AELLCLDARHREESCGGHFRSEYQYPDGEAKRDDENFCYAAAWEYTGNFGAPTLNKEP
                     LSFEEVHLAVRSYK"
     gene            7642..7929
                     /locus_tag="PLP58_08715"
     CDS             7642..7929
                     /locus_tag="PLP58_08715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ08635.1"
                     /translation="MTRESESELLSFCAAQRGDFCADAWTRFDRVEKREMAAVCLFLA
                     GVDWFGHRQQLEKIGRGLIEQANTSFAQLTSLLGFDCARFSNLLKRRIGHA"
     gene            7922..8506
                     /locus_tag="PLP58_08720"
     CDS             7922..8506
                     /locus_tag="PLP58_08720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ08636.1"
                     /translation="MHDLHPELTQAMSTLMDKLDTVLRSGGYAGAPLQMYLAGGMAVH
                     YHCGTRYTEDVDASFSHRLLLPYKELVVEYLREDQTRSSIYLDPTYNDTFALLHPDHR
                     DASVEWQDIGNERRLIHLRVFCPVDLAVSKLSRFTSIDQQDIRDLARHRYFTSPELQK
                     RATEALDCYVGDTRWIHLNLRQISEEIDQLALPS"
     gene            8533..9321
                     /locus_tag="PLP58_08725"
     CDS             8533..9321
                     /locus_tag="PLP58_08725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018968669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase/fumarate reductase
                     iron-sulfur subunit"
                     /protein_id="HRJ08637.1"
                     /translation="MSQLNLHLKVWRQNAGQPGHFQDIDAPDIPDHASFLEMMDIVNE
                     RLISKGQEPVAFDHDCREGICGMCSMQINGVPHGPEKATTTCQLHMRKFRTGDTIWIE
                     PFRAKAFPVIKDLVVDRGAFDRIQQAGGFVSVRTGAPQDANALPIGKEIADAAMDAAE
                     CIGCGACVAACKNASAMLFVSAKAGQLNLLPQGKPEKDRRTLGMVRQMDKEGFGNCTN
                     QYECEAACPKEISVRWITKLNRDYAVAAVKEALGNSPKVAGGGD"
     gene            9376..10626
                     /locus_tag="PLP58_08730"
     CDS             9376..10626
                     /locus_tag="PLP58_08730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ08638.1"
                     /translation="MRFLSCLLPLIVLPAALTALETKVAFREAPHKYLQHEPQDRFAK
                     LKKQIESGEVRLDTSNDKAFLASVLKALDVPVSSQLLVFSASSLQSEIINPRNPRALY
                     FNEDTYVGWVPRGKVEIIAMDPEMGAMFYIFERLNAGGGVPPITRSDKCFNCHAGLAT
                     RRVPGLIAESLLPMLSGASLETYRRDEQGHHIPLEKRFGGWHLTGGHHLKTHHANMMG
                     TNVPGRGIEKSKVEPGQMSDLGQHLLPTSDILPHLVHEHQIGFENRVFHAAYVMRQLL
                     AEGRGSLPMSAKPELEELAEELARYILFVDEAKLPKEGVEGDTEFIREFQRNKREAAG
                     GRSLKDFDLKTRIFKYRCSYMLHTESWLRLPVVLKDRVYFKMAEGLREQNANPVYSHL
                     AADEKLAIRAILKETLPGLPSWWR"
     gene            10782..11165
                     /locus_tag="PLP58_08735"
     CDS             10782..11165
                     /locus_tag="PLP58_08735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009959625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HRJ08639.1"
                     /translation="MKKLIALALSMFAASAMAAEFPDISIADLKAAMEKKEVVIIDVN
                     GAASYKAGHIPGAVDFATKGADLGSALPSNKDALVVAYCGGPSCSAYKRAAQKAAELG
                     YTNVKHLSAGKSGWAKAGETLEPAK"
     gene            11245..11553
                     /locus_tag="PLP58_08740"
     CDS             11245..11553
                     /locus_tag="PLP58_08740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ08640.1"
                     /translation="MKKLILSLGLLLSAAALADDSKAVTFEGKITGVVCASCKAHITA
                     ALTQKLPDVVSVDVKPGEDAESQKIIIITRDESLTKDKAVDALGTYAKNYQILSLAKK
                     "
     gene            complement(11566..12795)
                     /locus_tag="PLP58_08745"
     CDS             complement(11566..12795)
                     /locus_tag="PLP58_08745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009962287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo acid dehydrogenase subunit E2"
                     /protein_id="HRJ08641.1"
                     /translation="MPAIPILMPQLGESIAEATIIRVLAGPGQEVEAGSDLFEVETNK
                     ATMAVAAPCAGRVIQITAEVQQSYAVGSNLASFEISAEDAGALGLNENKEPPASDASA
                     PSREGESLHFQFNEEDNIRDYQPKVEPVVGGLPVPAGATGASYISPRMRARMNELGLN
                     ASDLAGIAGTGAGGRVTVEDFENFLRSLEQHRLTPASPMRIAVADSMRRSWSRPLATV
                     GSPVILDAVLAHRKKAEAKPGPALYAIRALALALAENTAVAGRLIGNRIVHPRAIDIG
                     FAVEVEDGVLVPVLREVEKTPLSKLLPTYNKLVEQARARRLPHDSSRPGIATVTNFGT
                     FGIVWATPIPLPEQNLVLGLGAGSKVPRWSDEVNQFIPVTEAELTLSFDHRILDGGGA
                     GRLLARVAKLLQEPEKL"
     gene            13290..15029
                     /locus_tag="PLP58_08750"
     CDS             13290..15029
                     /locus_tag="PLP58_08750"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing sensor histidine kinase"
                     /protein_id="HRJ08642.1"
                     /translation="MAKTAGRVAQSAAQVQSVGLVWASGGGFQQTSCCAPDTINGFKL
                     SSGHDDKIKLALSGQESWCEVKGERNEAKAVFGIIPVAHADGRVCAAAVVEYSAKAWN
                     QVRNMAQWQIAGEFTGLLITFVCMGMLLVARSEQRRQRAVEEATKPLLDELESLDGMV
                     NSMHGVVWEREEGARDFGFISEQADGFLGYPLARWTEEEGFFDSIILPGDRQRVLKSR
                     KDAEKTRSRYEVEYKVMTADGKTAYVSEHAKAEKPPFDSVVVRGILVDVTRRREAEQV
                     NNEMHKMMLEASRQAGMAEVATGVLHNVGNVLNSLNVAAKLLSERLQKSRFDKLCQAT
                     DLLKEHLPDNPEFFTTDSRGQALPGYLADLSRYLREEQGRLSETTSDMIQRIEHIRDM
                     IMLQQMHSAVRPLLESLDLATVVDEAVRLEMSVYGSHQEIRIERQYADLPQVYLPRSL
                     LLQILVNLVANACQAMAKTPDEARLLTLRVGPLGEEKVRISVEDNGCGIDARHLTKIF
                     KQGYTTKKDGHGFGLHHASLLAADMGGELRAESDGLGKGARFMLDLPAAKDPGCKAPA
                     HATPALEESITSP"
     gene            15026..16918
                     /locus_tag="PLP58_08755"
     CDS             15026..16918
                     /locus_tag="PLP58_08755"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HRJ08643.1"
                     /translation="MKPHPQSTISAVDFAEKDPLRVNQRILIVDDNSAIHEDFRKILS
                     PADYGEADLDLAESAVFGHEIKGDTVFDFQLAFAAQGEGAVKLAEMALQQGRPFALAL
                     VDVRMPPGMDGVETIKALWKIQPDMQIAICTAYADYSWDDIARHLGVTHRLMILKKPF
                     DPIEVIQIANALTTKWAYERESALRQLGLQTRLWEHTRRIEDACSRLNKEHQERLRLV
                     EDMRRMQKMDSLGALAAGIAHDFNNVLTVIQSQLSMSLMSAEQPQGIAESMEEVLLAA
                     RHAADLTDRLLKFSRREFMPPVPLQLDQAIENETALLKRSLGPQITLEISHAPDLPDV
                     VADPGSLGQIIINLALNARDAMPQGGRVAISSRFMRVENPEDAKSLHAEARAGEFAVI
                     SVSDTGTGMPPEVLDRIFDPFFTTKGAGRGSGMGLFVVRGLVRHMGGWVTVSSVPGVG
                     TDFQIYLPVIGSELPADDSEKPGRDFDGLSMGVPPCTILIVDDDSSVRHVVSSVLRAQ
                     GHSVLTAKDADEAWKCWASHRQGIHLVIADINLPGKSNGFDLGRAVLAEDSSVPFIFT
                     SGSCPDIVGQTGKLQLGINYLPKPFDVLDLLNAVGHALADTPSCDKPPAPAKTQTAKI
                     ELARIS"
     gene            complement(16928..17551)
                     /locus_tag="PLP58_08760"
     CDS             complement(16928..17551)
                     /locus_tag="PLP58_08760"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HRJ08644.1"
                     /translation="MIRLECYTGGIAMTNGWLVRLEGADLLVDAPEGVAGWLDERGAR
                     VSALLLTHQHFDHVMDAAEVKKRYGCPVLAWSGFSRALTLERLFGAFSGSLLTVPEFE
                     VNEVLEGREAAAIGGLEWRLLHIPGHSADSVCFHQPGQGIVFGGDVLFQGGVGRTDLP
                     GGSFEQLLSGIQAKLLPLPDATRVCPGHGNPTSIGDERLGNPFLTDY"
     gene            17658..19955
                     /locus_tag="PLP58_08765"
     CDS             17658..19955
                     /locus_tag="PLP58_08765"
                     /EC_number="3.4.21.102"
                     /inference="COORDINATES: protein motif:HMM:TIGR00225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxy terminal-processing peptidase"
                     /protein_id="HRJ08645.1"
                     /translation="MTRTLPLFLGAGLALLAPVTHQVRAETNFGQVAMHVAYMLQNQH
                     YSRQDFDDAMSARMLDNYLAMLDPRRVFFTAKDVKEFEAKYKTTLDDLILMRDISAAE
                     EIYGIYKQRVKNRVEFVRKTVEGRKFTFDSDRTTLIKRDKAPYPADSAAQDKLWLDII
                     EENLLQEILADQAKAEEKAKKPAKAADKPSDGKEDAPPVEKPASEEPEKTPEERIIKD
                     YERLIESVEENDYEDVVDLFLSSLSAAYDPHTEYMSVNEMDNFNIQMKHKLVGIGALL
                     GLQDDVAQIQGIVVGGPADKQGELKLNDKVTGVAQGEADFVETKYMKLQKIVDMIRGT
                     EGSIVRLRVNPAEDPSSTKIITITRGPVELKEKLANAELILTPSELGKPMKVGWINLS
                     SFYADMEDGTVSTTDDVQKLLARLMKEGMDGLVLDLRGNGGGSLEEAIRLTGLFVPAG
                     PVVQAKDWRGSITWRECESPKAFYEGPMIVLTDKTSASASEILAAALQDYRRALIVGD
                     KSTFGKGTVQTILPVDRFMPFFAKKDRAGNLKVTIQKFYRIAGGSTQLKGVEPDLVLP
                     SIRDVLDIGESSTENPLPYDTIPARKYNLVSQHPLPVAELSSRLAARLGANPEFQYIL
                     DESKRLKERIDRNSVAINLSQRQQETAENKARREKQEAERAERAKEVAARLGDETFKL
                     YHLTLDNVNDPELVPQSAFTREQSTGMKMAAKKDGEDDPNDMSKFPYGIEPARLETLH
                     ILRDFLELTGKQPTTAKTEGKAPAN"
     gene            complement(20043..21053)
                     /locus_tag="PLP58_08770"
     CDS             complement(20043..21053)
                     /locus_tag="PLP58_08770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009962282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaK"
                     /protein_id="HRJ08646.1"
                     /translation="MSKNTAKKIINDPLKCADELFEGLVLAYDGKARRAGKRSIVMND
                     LRPDAPGLLIGGGAGHEPIYHGLVGKGMGDGAAVGDIFAAPPPDIVLEATQAVNRSKG
                     VLYLYGNYAGDVMNFDIGAELAEEEGIQVKTVIINDDVCSAPPTEKGKRRGIAGLVPI
                     VKLAGAAASQVDTLDELARIAQKAVDMTRTVGVSTKPGSIPATGAPTFELADDLIGLG
                     MGIHGEKGVGLIPMCPADELAVKILDLLFADDLPLGAGDEVVFFVNSLGSTHLMELLI
                     MLRAARPILEARGLRIHQTICDNIVTCQEMAGVSFSITKLDAELKRLWDLPCESLGYT
                     KL"
     gene            21332..22468
                     /locus_tag="PLP58_08775"
     CDS             21332..22468
                     /locus_tag="PLP58_08775"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HRJ08647.1"
                     /translation="MTWVLCGLLALVVYTHAGYPLLMAVLARWRPRALDPHEDDKAGL
                     SIVVCARDAGAAITARLENLLACVWEGELEILVFCDGCADDTAARARALGDARVRVIE
                     SPEPRGKAAALNEAIPACRFAQVVLCDVRQRFHPEALRHLTRPLLDPDVMAVSGLLEI
                     EPSAAGGGQGVDVYWKMERRLREWEGRYDSVIGCTGAICAIRRAAFTPLEPDTILDDV
                     EIPMRMAVAGGRVFFEPRARAFDPQTLDPAREKARKLRTLVGNYQLIARYPRWLLPWR
                     NRLWWQLISHKYLRLAVPWLLIGIAVCSLMAPPLPWVRALVAGQALAYACALAGLLMP
                     RLRWKVLSIPAGFVLLQWSCLQAFFAFLRHRRDLRALWKTPARS"
     gene            22465..>23496
                     /locus_tag="PLP58_08780"
     CDS             22465..>23496
                     /locus_tag="PLP58_08780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ08648.1"
                     /translation="MIRALDRWLPSYLLRDRRRPAATAENPAHVFLAVCDHFEPFHHT
                     DKAGALRALSDWEALWPPLVEAFRDSSGRGPRHSFFYPVEQYDEEVIERLAALCEKTG
                     SEVEVHLHHENDTAETLTAQLRAGTANLARHGLLSRAADGAPRYAFIHGNWALDHSHP
                     RGRHCGVSDELAVLRRTGCYADFTLPSAPDPAQTRTVNSVYYAREDGLPKSHDRGIAA
                     RSGVTGGLAASDEHLLLIQGPLGLNWRRRKWGLLPRLENGDLTGANPPTPGRFQLWTE
                     ICPHVKDGPPWIFVKLHTHAGIPRNYRSLLGDAGRHFHEGLAARARQDPALRCHYVTA
                     REMVNLVHAA"
BASE COUNT         4536 a         7712 c         6945 g         4303 t
ORIGIN      
        1 agcggtgccg gcttccgcct gcggcccgcg cgcgcggcgc aagcaagata agggcaccct
       61 aaagcaaggc acggcgagcg cgcgcgaatg acgcgcgctt ctgtggcggc gtcatggaag
      121 tctttgccgt caccatcttc atgagcctcg ccttcgcggt gctcttcgcc gtccttttcc
      181 tcgccgagcg cggccagcgc cagcgccgct ccgtggagca gatgtccctg ctgccgctgg
      241 aggccgacca ggaccatgcc ggacgctgag actccccatt tcccccgccc atgacctccg
      301 cctccaccgc caagaccctc atcgagttcg acgaccgcac cgtccgccgc ttcatgatcg
      361 cctccatcat ctggggcgtc gtgggcatgc tggcgggcgt cttcatcgcc ctccagctca
      421 acttccatga gctgaacctc gcctcctggc tcagcttcgg ccgcctgcgc ccgctgcata
      481 ccaatgccgt catcttcgcc ttcgtcggca acatgatgtt cgccggcatc tactactcca
      541 cccagcgcct gtgcaaggcg cgcatggcct ccgacctgct ctcgaacatt cacttctggg
      601 gctggcagct catcattgtc tccgccgccg tcaccctgcc gctgggcttg acgcgcgggc
      661 aggaatacgc cgagctgatc tggccggtca acatcgccgt ggcgctcatc tgggttgtct
      721 tcgcggtgaa tttcttctgg acgctggcaa agcgcaacga gccctccctc tatgtggcgc
      781 tgtggtttta catcgccacc atcatcacgg tggcgatgct ctacatcgtc aaccacatct
      841 ccatccccac gagctggacg cacagctaca ccatcttcgc cggtgtgcag aacggcctcg
      901 tgcagtggtg gtacgggcac aacgccgtcg ccttcttcct gacgaccccc atcctcggca
      961 tcatgtatta cttcctgccg aaggcggtgg agcggccggt gtactcctac cggctttcga
     1021 tcgtccactt ctggtccctc gtcttcatct acatctgggc cggcccgcac catctattaa
     1081 acaccgcgct gcccaagtgg ctgcaaatgc tcggcatgtt cttcagcctc atgctctggg
     1141 cgccgagctg gggcggcatg ttgaacggcc tgctcaccct gcgcggcgcg tgggacaagc
     1201 tgcgcaccga ccctgtggtg aagttttaca tcgccgccgt cacgttctac ggcatgtcca
     1261 ccttcgaagg tccgctgctc tccatccgcg ccgtcaacgc cctctcccat tactccgact
     1321 ggaccatcgg ccatgtgcat agcggcgcgc tcggctggaa cggactcatg gccgcgggca
     1381 tgttttactg gctgacgccg aagctctacg gcggcaagct ccactccgtg gcctgggcga
     1441 acttccactt ctggatcgcc accatcggca tcctcatcta cgttgccgcc atgtgggtct
     1501 ccggcatcat gcagggcctc atgctcaacg ccacgaacgc cgagggcacc gcgctcgttt
     1561 acccgaactt catcgagacc ctcacctcca tccgcgccat gatgggcttc cgcatcatcg
     1621 gcggcgcgct ttacctggcg ggcttcatcc tcatgggcgt gaacctctgg ctcaccatcc
     1681 gcaaaggcaa acccgtcaac gaaacgcgcg aagtcgccgt catccagccc aaggccaagg
     1741 acaccatggg catgagggac accttcatca acgaccccgt cgcctatatg ttcgccggca
     1801 tggtcctcgt gctcgcctgg cttttcctgc cgcccggcgc cgacatcgcc gcgctccttt
     1861 gtgccggcgt cttctccgtc ctggcggtga aaaaatttcg tgagggccac tcccgctggt
     1921 cctcctggta tgagggcctg ctgaacaact ggttgccctt caccgtgctc accttcgtcg
     1981 ccgtcgccct cggcggcctc atccagatca ttcccaccgt caccgtcaac cgcgccaaaa
     2041 acgtcgagga ccgcatccag caagcctaca ccccgctcga gctcgccggg cgcgacatct
     2101 acgtcagcga aggctgctac aactgccaca gccagatgat ccgcaccatg ctgccggacg
     2161 tgctccgcta cggcgactac tcccgccttg gcgagagcat ctacgaccac cccttccagt
     2221 ggggatcgaa gcgcacaggg ccggacctgg cccgcatcgg cgacaagtat ccgcatagct
     2281 ggcactacga ccacatgatg gagccgcgct ccacctccgt cggctcgaac atgcccgcct
     2341 acccccacct ctttgagcag aagttcgacc aaaaaagcct gcccaaaaag atcgccgtca
     2401 tgacccggct cggcgtgccc tacccgccga tgagcgccga cgagatcaaa atgaaagccc
     2461 tcgaacaggc cgtcaaaatt gcggaggaac tcaaagccca gggcaaagtg gccgcgcccg
     2521 acacccagat cgtcgcgctc atcgcctaca tgcagaagct cggcaaatac gacacccccg
     2581 aggtcgagaa aaagctggcg ccgcaaggcg tgcccttccc gctcaagccc gcgctgcccg
     2641 acagccaccg caaatccgtc acccaaaacc cctgatccgc ctccgccatg tacaaacgcg
     2701 tgctttatga aaactgggcc gactgggtgc cctacctcgc cttcggcatc accgccctcg
     2761 tcttcatctc cttcgtcgtg cgcgccgtca ccctgcgcaa ggagcgcgcc gaccagatgg
     2821 cccgcatgcc cctcgaagac tgacccccca ttttccctcc ccatgtcctc ccacaatgac
     2881 acctcagacg gccccatcct gcgcgaccac gtctatgacg gcatccagga atacgaccag
     2941 aagctgccca actggtggct ctttacctgg tacatcacca tggtctggtt cgtcatcgcc
     3001 tgggtcgcct actaccagct cggccgcggc gactcggatg tggagcagat agacaaagcc
     3061 atcgccgccg tcgaaaaaca ccgcgacgag gaactcgccg cgctggacga cgacaagctc
     3121 tgggaaatgt ccaaggacgc caaagtcgtc gaagccggca aagccatcta ctccaccacc
     3181 tgcgtggcct gccacgcgcc cgacctcagc gcccacatcg ccggagtcaa gctgcccggc
     3241 ctgcctctca atgacagcga gtggaagcac ggcggcgccc ccacgcaaat cctgaacatc
     3301 atccgcaaag gcgcgcccga cctcaccaaa ggcatgcccc cctgggagcc gcagctcggc
     3361 atccagcgcg tcgtcgaggt caccgccttc ctcctcagcc atcacgagaa aggcaagccc
     3421 gtcaccctcg ccgccgactc gccattgaag acgggaggat agtctcctga tcgcccggcg
     3481 ctcagcacgc ttgccttcgg cccgcccctg gtggtaaaat ccgctccatg agagcactcc
     3541 acctcccgcg cgccgccgcc cgcatgaagc ccgtcatggc agggatgttt tttttggcgg
     3601 cggccctccc agcccaggat gggccgcgca agtggactga cgcgcagggg cgcacggtgg
     3661 aggcggagtt cgccgggctg gacggggaga ccgtgctttt gaaaatgagc ggcgggcaga
     3721 ccgtgccgta tccgctggcg aagctcagcg cggaggatca ggcgttcgtc tcgaagcgca
     3781 aggaggcgtc cgccccggcc gggccgtccg cggcggggcc gaggcagcct ttggaaaagc
     3841 gggagtggcc cggcaacgtg gccgtgtcca cgcgcgccat tgagatcaag gtggtcgagg
     3901 agaaagccgc cgagcgccgt tatgtgtacc agtcggaggc tttcgagttc acctcccagg
     3961 cgaagctggc cggcagtgtc atgaccgagg tggcgcgcac cttcgaagcc acgcgcgcgg
     4021 tcatcaacgc gctgccctgg ggcgttgtct gccagccgcc ggacggcatg ccccgctttc
     4081 tcgccgcgct ttacgagacg cggcaggatt atgtggacgc gggcggaccg gagctttccg
     4141 gcggcgttta ccagacagcc gagaagactt tccgcatccc ttttcagagc ctcggcctgg
     4201 agaagcgcgg acagacgtat tttaaaaacg acttctccaa cgacaccctc gtgcatgaga
     4261 tcacccacca gatgatgcac gactacctcg gtttcctgcc cacctggctc atcgagggca
     4321 tggccgagta tgtggagctg ctgccctacc gcgcgggagt cttccgggcc gacgctcaca
     4381 agacagcgat gaagtccacg gtggatgagt ggatcaagag cgggcgtccg ccgatgatac
     4441 ccagcctgga ggcgcacatg agcatgaacc gcgagggctg gggcgcggaa acggcggagg
     4501 cggagaaaat gcgcgagatg taccgccgct cgatccttct tgtctattat ttcagccatc
     4561 tcgacggtga caggacaggc gcgcgttttc tgcgcttcat ggaggctgtg tacggcgagg
     4621 tggaggcgat gcgcgcgttt ttcgccgacc ccagggtgaa gcgcatggag ggcggccgct
     4681 tcacctaccc gtcctcgctc aaacctccgg gcatgtacaa ggacgagacc atcttcaggc
     4741 atctcggaat cctggtggat gagcgcccgt atgaaaaaat cgccgctgac atcgtcgagg
     4801 gttacaagac catcggcgtg aaagtggatg tgcggccttg agcgcgtctg ccgcagggca
     4861 aaatgcgggc ggaaggccgc aaaatcaggc tttccaggga aaaaatccag gtgcgtgttg
     4921 ccacccgtcg gcgtatcgcc atgatgaccg tcatcccatc ccatgagcgc tgtttttgac
     4981 tcgatctcgc gattttattc ctcctccatc ggcaagaaga tcctcgtcgc cctgacgggg
     5041 gctgcactcg cgctctttgt cctgggccac atgatcggaa acctgctcgt tttcgccggg
     5101 cctgacgcca tcaatgagta tggccacatg ctccagaccg ctctccatgg cggaggggtt
     5161 tggatcgccc gcatcggcct gctggccgcc gtggcggtcc atgtggtggc gaccgtttcg
     5221 ctgacaaagg cgaacaaggc cgcccgcccc gacgcctacg gccagcacaa ggcgcaggtg
     5281 agcggcaggt cctcccgcat catgatctgg agcgggctta cggtgctcgc tttcatcatt
     5341 ttccacctgc tgcattttac aatccgggcc ggcaacgact tcaacggcgc ggcttacaaa
     5401 accgagcttc atggcgagac ggttcacaat gtcttcaaaa tggtgataga cggtttttcc
     5461 tcgacgccgg cctcgatttt ctacctcatc gccatggcgc tgctgtgcag ccacctgagc
     5521 cacggcttta gcagcctctt tcagacgctt ggcatcagca cggacaagac cgagccgctt
     5581 ttcaaaaagg ctggttatgc cttcgcgggc ctcatcttcg ccggaaacgc cgccatcgtc
     5641 ctctccatct ggctcttcgg ctacggccgc tgattctgac tcttcgccct ctgccctaag
     5701 ctctccgcct tttccgccat gtctctcgac tctcacattc ctagcggccc gatggccatg
     5761 aagtggtcca agcacaagca ggactcgaag ctcatcaatc cgaagaacaa gcgcaagttc
     5821 accgtgctcg tcgtgggcag cggtctcgcg ggttcttccg ccgcggcgac gctttccgag
     5881 ctgggctaca aggtgaagtg cttctgcttc caggactcgc cgcgtcgggc gcactccatc
     5941 gcggcgcagg gcggcatcaa tgcggcgaag aactatcaaa acgacggcga cagcgtgcat
     6001 cgcctctttt atgacacggt caaaggcggc gacttccgcg ctcgcgaggc caatgtgcat
     6061 cgtcttgccg aggtcagcgt gaacatcatc gaccagtgcg tggcgcaggg cgttcccttt
     6121 gcccgtgaat acggtggcat gctggcgaac cgttccttcg gtggcgctca ggtgagccgc
     6181 acgttttatg ctcgcggtca aactggtcag cagctcctgc tcggtgccta ctcggctttg
     6241 aacaaagaaa tcgccgccgg cggcgtgcag atgtatccgc gcacggaaat gctcgatgtc
     6301 gtcatcgtgg acggccacgc gaagggcatc atcacgcgca atctcgtcac cggcaaaatc
     6361 gaagcgcacg cgggcgacgc ggtgctgctg tgcaccggtg gttacggcaa cgtgttctat
     6421 ctttcgacca acgcgatggg ctgcaacgtc accgcgacgt ggcgtgcgca tcgcaagggc
     6481 gcgttcttcg cgaatccctg ctacacgcag attcatccga cctgcattcc ggtcagcggc
     6541 gactatcaga gcaagctcac gctcatgtcc gagtcgctga gaaacgacgg ccgcgtgtgg
     6601 gtgccgaaga agaaggaaga ttgcggcaaa gcgccatcgc agatcgccga ggcggatcgc
     6661 gattactacc tcgagcgcaa gtatccgagc ttcggcaacc tcgccccgcg tgacatttcc
     6721 tcccgtgcgg cgaaggaagc ctgcgacgac ggtcgcggcg tcggccccgg cgggcgcggc
     6781 gtgtatctcg acttcgcgga ggccatcggc cacttcggtg cgaagacgat cgccgagcgc
     6841 tacggcaatc ttttcgaaat gtatgagcgc atcaccggcg agagcgccta caagcagccg
     6901 atgcgcatct acccggccgt tcactacacc atgggcggcc tgtgggtgga ctacaatctc
     6961 atgagcaacc tccccggcct ccacgtgctc ggcgaggcga acttctccga ccacggcgcg
     7021 aaccgtctcg gtgcctccgc tctcatgcag gggctcgccg atggctactt tgtcatcccg
     7081 acgaccatcg cgaactacct cgtgcatcaa aagcccggca gcgtcaccgc cgagcatccg
     7141 gagttcaaga aggcgctcga agtctgcacc gcgcagacga agggcttcct caacagcaag
     7201 ggcaagcgca gcgtggacag cttccaccgc gaactcggtc tcctcgtctg ggacaagtgc
     7261 ggcatggcgc gcacgaagga aggcctcacc gaagccttga agcgcatccc cgagctgcgc
     7321 gaggaattca aaacgaatct cctcgtcccc ggcagcggcg acgcgtgcaa tcccgagctc
     7381 gaaaaagccg gacgcgtggc tgatttcatg gaattcgccg agctgctctg cctcgacgcc
     7441 cgtcatcgtg aggaaagctg cggcggtcac ttccgcagcg aataccaata cccggatggc
     7501 gaagcgaaac gcgacgacga aaacttctgc tacgccgccg cctgggaata caccggcaac
     7561 tttggcgccc ccacgctcaa caaggagccg ctctcctttg aagaagtcca cctcgccgtg
     7621 cgcagctaca agtaaaacgc catgacccgc gaatccgagt ccgaactgct gagtttctgc
     7681 gcggcgcagc gcggggactt ctgcgcggac gcgtggacgc ggtttgatcg tgtcgagaaa
     7741 cgtgaaatgg cagctgtgtg cctgtttctc gctggcgtgg actggtttgg gcatcgccaa
     7801 cagctcgaaa agatcgggcg tggcttgatc gagcaggcca acacgagctt cgcgcagttg
     7861 accagcctgc ttggcttcga ttgcgcccgt ttttccaacc tgctcaaaag gaggatcggc
     7921 catgcatgat cttcatcctg agctgacaca ggcgatgagc actctgatgg acaagctgga
     7981 cactgttttg cgaagcggcg gctacgcagg agctccgctc cagatgtatc tcgcgggcgg
     8041 catggcggtt cattaccact gcggcacccg ctacaccgag gatgtcgatg ccagcttctc
     8101 ccaccgcctg ctgctgccct acaaagagct ggtggttgaa tacctgcgtg aagatcagac
     8161 gcgctcgtcc atctatctcg atccgaccta caacgacact tttgcactgc ttcacccgga
     8221 tcatcgcgat gcatccgtgg aatggcagga catcggcaat gaacgccgcc tgattcatct
     8281 ccgcgtcttt tgtccggtcg atctcgccgt ctcgaagctc tcgcgtttca cgtccatcga
     8341 ccagcaggac atccgcgacc tcgcgcgtca ccgctacttc acctcgcccg aattgcagaa
     8401 acgagccacc gaggcgctcg actgctacgt cggcgacacc cgctggatcc atctgaacct
     8461 ccggcagatc agcgaggaga tcgaccagct cgccctgccg tcctaatttt tcacgcccac
     8521 tcccttccac tcatgtccca actcaatctt cacctcaaag tctggcgtca gaacgcgggt
     8581 cagcccggcc acttccagga catcgatgcg ccggacattc cggatcatgc ctcgttcctc
     8641 gaaatgatgg acatcgtgaa cgagcgcctc atttccaaag gccaggagcc cgtcgcattc
     8701 gatcacgact gccgcgaagg catctgcggc atgtgctcca tgcagatcaa cggcgtgccg
     8761 catggcccgg agaaggccac gacgacctgc cagcttcaca tgcgcaagtt ccgcacgggc
     8821 gacacgatct ggatcgagcc cttccgcgca aaggccttcc cggtcatcaa agacctcgtc
     8881 gtcgatcgtg gtgcgtttga tcgcatccag caggccggtg gtttcgtcag cgtgcgcacc
     8941 ggcgctccgc aggatgcgaa cgcgctgccc atcggcaaag aaatcgccga cgccgcgatg
     9001 gatgccgccg agtgcatcgg ctgcggtgcc tgcgtggcag cgtgcaaaaa cgccagcgcc
     9061 atgctcttcg tctccgccaa agccggtcag ctcaatctcc tgccgcaagg caagcccgag
     9121 aaggaccgcc gcacgctcgg catggtccgc cagatggaca aagaaggctt cggcaactgc
     9181 accaaccaat acgagtgcga agccgcctgc ccgaaagaga tcagcgtccg ctggatcacc
     9241 aagctcaacc gcgactacgc cgtcgctgcc gtcaaagaag ccctcggcaa ctcgccaaag
     9301 gtcgcgggtg gcggggattg agccgctgcg gctgaaattc acataaaaag ggagacttgg
     9361 cgcgttttga gggtcatgcg ttttttgtct tgcctccttc cattgatcgt cctgccggct
     9421 gcgctgacgg cgttggagac caaggtggca ttccgcgagg ctccgcacaa atatctccag
     9481 catgagccgc aggaccgttt tgcgaagttg aaaaagcaga tcgagtccgg cgaggtcagg
     9541 ctggacacct ccaatgacaa ggcctttctg gcctcggtcc tgaaagctct ggatgtgcct
     9601 gtcagttccc agcttttggt tttctcggcc agttcgcttc aaagcgagat catcaatccg
     9661 cgaaatccgc gcgcgctcta tttcaacgag gacacctatg tgggctgggt gccgcgcggc
     9721 aaagtggaga tcattgccat ggacccggaa atgggggcca tgttttacat ttttgaacgg
     9781 ttgaatgccg gcggaggagt gccgccgatc acccgttcgg acaagtgctt caactgtcac
     9841 gccggtctgg caacccggag agtgccggga cttattgccg aatcgctgct gcccatgctc
     9901 agcggcgcga gtttggagac ctaccggcgg gatgagcagg ggcatcacat tcctttggag
     9961 aagcgttttg gcggctggca tctcacgggc gggcatcacc tcaaaacgca tcatgccaac
    10021 atgatgggga ccaacgtgcc ggggcggggg attgaaaaat ccaaggtcga gcccggccag
    10081 atgtccgatc ttgggcagca tttgctgcca acgagcgaca ttctgccgca cttggtgcat
    10141 gagcatcaaa tcggcttcga gaaccgtgtt tttcacgccg cctatgtgat gcggcaattg
    10201 ctggcggagg gcaggggaag cctgccgatg tcggcgaagc ctgagttgga ggaactggcc
    10261 gaggagctgg cgcggtacat cctctttgtg gacgaggcga agcttccgaa ggagggcgtc
    10321 gaaggggata cggaattcat tcgcgagttt cagaggaaca aaagggaggc tgctggcggc
    10381 aggtctctca aggactttga ccttaagacg cgcattttca aataccgctg cagttacatg
    10441 ctccacaccg agtcctggct caggctgccg gttgtattaa aagaccgggt gtatttcaaa
    10501 atggccgagg gtctgcgcga gcaaaacgcc aatccggtct attcccactt ggcggctgat
    10561 gagaaactgg ccattcgcgc catcctgaag gagacgctgc ctggtctgcc gtcctggtgg
    10621 aggtgagttt tttcaatgaa tcctctccct gcggcgtttt atcctcactg ttcttccttc
    10681 ccgggaataa acccccggcg gaggagtcac actgcccgcg cgggcagtcc aaactggata
    10741 ccgcgcattt caaaccacaa tccatcctga accaccaaac tatgaaaaaa ctgatcgcac
    10801 tcgcactctc gatgttcgcc gcctcggcca tggccgctga attcccggac atcagcatcg
    10861 ccgacctcaa agccgcgatg gaaaagaagg aagtcgtcat cattgacgtg aacggcgccg
    10921 cctcctacaa ggcgggtcat atcccaggag ccgttgactt cgccaccaaa ggcgctgatc
    10981 tgggctccgc cctgccatcc aacaaggacg ccctcgtcgt tgcctactgc ggaggcccct
    11041 cctgcagcgc ctacaagcgc gccgctcaga aggccgccga actcggctac accaatgtga
    11101 agcacctctc cgccggcaaa tccggctggg ccaaggcagg ggagacgctc gaacccgcca
    11161 aataaatcgc tttgaggaag ccgggatgcc gttggacaaa gcggcgtccc ggcttttttg
    11221 ttcgagcctg catatccgca tcccatgaaa aaactcatcc tctctcttgg actcctcctc
    11281 tccgccgccg ccctggccga tgacagcaag gccgtcacct ttgagggcaa aatcaccggt
    11341 gtcgtctgcg cttcctgcaa ggcccacatc accgccgccc tcactcaaaa gctgccggat
    11401 gtggtcagcg tggacgtgaa gccgggcgag gacgccgagt cgcagaagat catcatcatc
    11461 acgcgtgacg agtccctgac caaggacaag gcggtggacg ccctcggcac atacgccaag
    11521 aattatcaaa tcctcagcct ggcgaagaaa taaggccgcc agggttcaca atttttcagg
    11581 ctcctgaagc agttttgcca cgcgcgccag gagcctgccc gcgccgccgc cgtcgagaat
    11641 gcggtggtcg aaactcaggg ttaactccgc ctcggtgacg gggatgaact ggttcacctc
    11701 gtcgctccag cgcggcactt tgctccccgc gccaagaccc aggacgaggt tctgctccgg
    11761 cagagggatg ggcgtggccc agacgatgcc aaaagtgccg aagttcgtga cggtggcgat
    11821 gcccgggcgg ctgctgtcgt gcggcagacg gcgggcgcgg gcttgctcga cgagcttgtt
    11881 gtaggttggc agcagtttgc ttagaggtgt tttttccact tcccgcagga cggggacgag
    11941 cacgccgtcc tcgacctcca cggcgaatcc gatgtctatg gcgcgcggat gcacgatgcg
    12001 gttgccgatg agcctgcccg cgacagcggt gttttccgcc agggccaggg ccagggcgcg
    12061 gatggcataa agcgccgggc cgggcttggc ctccgctttt ttccggtgcg ccagtacggc
    12121 gtcgaggatg accggactgc cgacggtggc cagcgggcgg ctccaactgc ggcgcatgga
    12181 gtctgccacg gcgatgcgca tgggcgaggc tggcgtcaaa cggtgctgct ccaggctgcg
    12241 caggaagttt tcaaaatcct ccactgtgac ccttcccccc gcgccagttc cggcgatgcc
    12301 ggcgaggtcc gaggcgttga ggccaagctc gttcatgcgt gcgcgcatgc gcggggagat
    12361 gtagctggct cctgtggcgc ctgccggcac gggcagaccg ccgaccacgg gctccacctt
    12421 tggctggtag tcgcggatgt tgtcctcctc gttgaattga aagtgcaggg actcgccttc
    12481 ccgggaaggc gcggaagcat cggacgccgg tggctctttg ttttcgttga gacccaaggc
    12541 cccggcatcc tcagcgctga tctcaaagga ggccaggttg gagccgaccg cgtagctttg
    12601 ctgcacttcg gcggtgatct ggatcacccg tccggcgcaa ggggcggcca ccgccatggt
    12661 ggctttgttg gtttccacct caaacaaatc actccccgcc tccacttcct ggcccggtcc
    12721 ggctaggaca cgaatgatgg tggcctcggc gatggattcg cccaactgtg gcatcaagat
    12781 gggaatggcg ggcatgatct gaggatggac agggtcagaa atggcggcaa cgcggctttg
    12841 agtcaagcta tggcgctgat ggcaggaatt gagaaaagac aggaatgcgg gtgatcatgc
    12901 ggagccaacc agaaaaaggg agagacaaaa aataaggacg attttttcga ataaaattat
    12961 tgtcattatg gtttttatat ctttcatttc tgataattat gcattgggac ataccggaac
    13021 tcatctttat agccaccgcg ctggcagtcg gcggggcgtg tcttggctgg ctgaaaaaga
    13081 ggtaccgaaa cacccaccac cacctgttga tcttctgcct cgttggagct gtggggtatc
    13141 tggctccgat gcacatggag cgcgcggcgg aacgcgcgcg ggaattgacc aagctttcct
    13201 ggcttggaat cgcccggacc tatgctcatg attttgaaag ggagcacaga gagccacagg
    13261 aatgggatca gccgggaata cgccggatca tggcaaaaac agccggtcgt gttgctcaat
    13321 ccgccgcaca ggtgcagtct gtgggcttgg tctgggcatc tggcggcgga ttccagcaaa
    13381 caagctgctg cgcgcctgac accatcaatg gcttcaagtt gagcagcggg cacgatgaca
    13441 aaatcaagct ggcgctttcc ggccaggaat cctggtgcga ggtgaagggg gagcgcaacg
    13501 aagccaaagc agtcttcggc atcatcccag tggcccatgc cgacggcaga gtctgcgccg
    13561 ccgcagtggt ggaatattcc gccaaagcct ggaaccaggt ccgtaacatg gctcaatggc
    13621 agatcgcggg cgagttcacc ggactgttga tcaccttcgt gtgcatgggc atgctgctgg
    13681 tggcgcgctc cgaacagcgc cggcagcgcg ctgtggagga ggccacgaaa cctttgctcg
    13741 acgagttgga aagcctcgac ggcatggtga actcgatgca tggcgtggtt tgggaaaggg
    13801 aggagggagc acgggatttc ggcttcatca gcgagcaggc ggacggcttt ctgggctatc
    13861 cgctggcgcg ctggacggaa gaggagggtt tcttcgactc gatcatcctg ccgggggacc
    13921 gccagcgggt gttgaaatcc cgcaaggatg ctgaaaaaac gcgctcccgc tacgaggttg
    13981 agtacaaagt gatgacggcg gatggcaaaa cggcctatgt ctccgagcac gccaaggccg
    14041 aaaaaccgcc tttcgacagc gtggtggtgc gtggaattct ggtggatgtg acccgaaggc
    14101 gcgaggccga acaggtgaac aacgaaatgc acaagatgat gctggaggca tcaaggcagg
    14161 ccggcatggc ggaggtggcc accggcgtgc tgcataatgt gggcaatgtg ctcaacagcc
    14221 tcaatgtggc ggccaaactg ctttccgagc ggctgcaaaa atcccgtttc gacaagctct
    14281 gccaggccac cgatctgctg aaggagcatc tgccggataa tcctgaattt ttcaccacgg
    14341 attcgcgcgg ccaggctctc ccgggctacc tcgccgatct ctcccgttat ctgcgcgagg
    14401 agcagggcag gctctccgag acgacctcgg acatgatcca gcgcatcgag cacatccggg
    14461 acatgatcat gctccagcag atgcacagtg ccgtgcgtcc cctgctggag tcgctggacc
    14521 ttgcaacggt ggtggacgag gcagtgcgtc ttgaaatgag tgtttacggc tcgcatcagg
    14581 aaatccgcat cgaacgacag tatgccgacc tgccacaggt gtatttgccg cgctccctgc
    14641 tgctgcaaat tctcgtgaac ctggtggcaa acgcctgcca ggcgatggcg aagacgcccg
    14701 atgaagcccg gttgctcacc ttgcgggtcg gccctcttgg cgaggaaaaa gtgcgcatca
    14761 gcgtggagga caacggctgc ggcatagacg ccaggcactt gacaaaaatt ttcaagcagg
    14821 gctacaccac caagaaagac gggcacggat tcggccttca ccacgcaagc ctgctcgcgg
    14881 ctgacatggg gggcgaactg cgcgccgaaa gcgacggtct gggcaaaggg gcgcgcttca
    14941 tgctcgacct gcccgccgcg aaagaccctg gctgcaaagc acccgcccat gccactcccg
    15001 cgctcgaaga atccatcacc tcaccatgaa accccatccc cagagcacca tctccgctgt
    15061 tgattttgcg gaaaaagatc ccctgcgcgt gaatcagcgc atcctgattg tggatgacaa
    15121 ttcagcgatc catgaggatt tccgcaaaat tctctcccct gcggactatg gcgaggctga
    15181 tctcgatctt gccgagtcgg cggtttttgg gcatgagatc aagggcgaca ccgtctttga
    15241 cttccagctc gcatttgccg cccagggtga gggggctgtc aaactggcgg agatggccct
    15301 ccagcagggc agacccttcg cgctcgccct ggtggatgtg cgcatgcctc ccggcatgga
    15361 cggggtggag acgatcaagg cgctctggaa aatccagccc gacatgcaga tcgccatctg
    15421 cacggcctac gcggactatt catgggacga catcgcgcgc catctcggtg tcactcatcg
    15481 tttgatgatt ctgaaaaaac ctttcgaccc catcgaggtc atccagatcg ccaacgccct
    15541 gaccacgaaa tgggcctacg agcgcgagtc cgcgctgcgc cagctcggtc ttcagacacg
    15601 tctgtgggag cacacccggc gcattgagga cgcctgcagc cgcctcaaca aagagcacca
    15661 ggagcggttg cggctcgtgg aggacatgcg ccggatgcag aaaatggact ccctcggcgc
    15721 gctggccgcc ggcatcgcgc atgacttcaa caacgtgctc accgtcattc aaagccagct
    15781 cagcatgagt ctgatgagcg ccgagcagcc tcagggcatc gccgaatcca tggaggaggt
    15841 gctgctggcc gcgcgccacg ccgccgacct gaccgaccgc ctgctcaaat tcagccggag
    15901 ggaattcatg ccgccggttc ctctccagct cgatcaagcc atcgaaaacg agaccgccct
    15961 gctcaagcgc tccctgggtc cccagatcac gttggagatc agccacgcgc cggacctgcc
    16021 tgacgtggtg gcggaccccg gttccctcgg ccagatcatc atcaatcttg cgctcaacgc
    16081 cagggacgcc atgcctcagg gaggcagggt cgccatcagc agccgcttca tgcgtgtcga
    16141 aaaccctgag gacgccaaat cccttcacgc cgaagcgcgc gcgggcgagt ttgccgtcat
    16201 cagtgtcagt gacaccggca ccggcatgcc gccggaagtc ctcgaccgca ttttcgatcc
    16261 ctttttcacc accaaaggcg cgggccgcgg ctcaggaatg ggtttgttcg tggtcagagg
    16321 gctggtgcgc cacatgggag gctgggtgac tgtttccagc gttcccggag tcggcacgga
    16381 cttccaaatt tatcttcccg ttattggctc cgaactgccg gcggacgact ctgaaaaacc
    16441 gggccgtgat tttgacggcc tctccatggg cgtgccgccc tgcaccattc tgatcgtgga
    16501 tgacgactcc agcgtgcggc atgtcgtttc cagcgtcctg cgagcccagg gccacagcgt
    16561 cctgacagcc aaagacgcgg acgaggcatg gaaatgctgg gcatcccacc gccagggcat
    16621 tcatctggtc atcgcggaca tcaaccttcc aggcaagtcg aatggctttg acctcggcag
    16681 ggccgtcctg gccgaggatt ccagcgtgcc gtttatcttc accagtggca gttgccccga
    16741 catcgtcggc cagaccggca agctccaact cgggatcaac tatctgccca agcccttcga
    16801 cgtgctcgac ctgctcaatg ccgtgggcca tgcgctggcg gacactcctt cctgcgacaa
    16861 acctccggct cctgccaaga ctcagaccgc caagatcgaa ctggcacgca tctcctgaac
    16921 cacggcttca ataatcagtc aggaatgggt ttcccagacg ctcgtcaccg attgaggtgg
    16981 gattgccgtg gcctgggcag acgcgcgtgg catccggcag cggcagcagc tttgcctgaa
    17041 tcccggacag caactgctcg aacgagccgc ctggcaggtc ggtccgtccc accccgccct
    17101 ggaaaagcac atctcctccg aagacaatcc cctgtccggg ctggtgaaag cagacactgt
    17161 ccgccgagtg gcccggaata tgaagaaggc gccattccag gccgccgatg gccgcggctt
    17221 cccggccttc cagaacctcg ttcacttcaa actcaggcac ggtcagcaga ctgccgctga
    17281 atgcgccgaa caggcgctcc aaagtcagcg cgcgcgaaaa gccggaccat gccagcacgg
    17341 ggcagccgta ccgctttttc acctccgcgg catccatcac atgatcaaag tgctggtgag
    17401 tgagcagcag cgccgagacc cgcgcgccgc gttcatcaag ccagccggcc acgccctcag
    17461 gcgcgtccac cagcaggtcc gcgccctcga gcctcaccag ccagccgttt gtcatggcga
    17521 tgccgcccgt gtagcattcc agtcgaatca tgaccggttt cctgcgggaa tccggtggac
    17581 atttcaagcc gcaaaaggca aattcgccga cgatttcgcc gttttaaatc gttatatcca
    17641 acgactgaaa gcccctcatg actcgcacat tacccctttt tctcggcgcc ggactggcgc
    17701 tgctcgcccc ggtgacgcac caagtccgcg cggagacaaa tttcggccag gtggccatgc
    17761 atgtggccta catgctgcaa aaccagcact actctcgcca ggatttcgat gacgccatgt
    17821 ccgcgaggat gctggacaac tacctcgcca tgctcgaccc ccgccgggtc ttcttcaccg
    17881 ccaaggatgt gaaggagttt gaggccaaat acaagaccac tctcgacgac ctcatcctca
    17941 tgcgcgacat cagcgcggcg gaggaaatct acggcatcta caagcagcgc gtcaaaaacc
    18001 gtgttgagtt cgtccgcaag acggtcgaag ggcgcaaatt caccttcgac tcggaccgca
    18061 caacgctcat caagcgtgac aaggccccct accccgccga ctccgccgcg caggacaaac
    18121 tctggctgga catcattgag gaaaacctgc tgcaggaaat cctcgccgat caagccaaag
    18181 ccgaggagaa agccaaaaaa cccgccaagg ctgccgacaa gccttcggac ggaaaagagg
    18241 atgcgccgcc cgtggaaaaa cccgcatcgg aggagcctga gaaaacgccc gaagaacgga
    18301 tcatcaagga ttacgagcgg ctcatcgaaa gcgtcgagga gaacgattat gaggacgtgg
    18361 tggacctctt tctctccagc ctttccgccg cgtatgaccc gcacaccgag tacatgagtg
    18421 tgaatgagat ggacaacttc aacatccaga tgaagcacaa gctcgtcggc atcggcgccc
    18481 ttcttggatt gcaggatgat gtcgcccaaa tccagggcat cgtcgtcggc ggacccgccg
    18541 acaagcaggg cgagctcaaa ctgaacgaca aggtgacggg tgtggcccag ggcgaggccg
    18601 actttgtcga gaccaaatac atgaagctgc aaaagatcgt ggacatgatt cgcggcaccg
    18661 agggcagcat cgtgcggctg cgcgtcaacc ccgccgagga tccctcaagc accaaaatca
    18721 tcaccatcac gcgcgggcct gtcgaattga aggaaaagct cgccaacgcc gagttgatcc
    18781 tcaccccgtc cgagcttggc aagccgatga aagtcggctg gatcaacctt tcatcgttct
    18841 acgccgacat ggaggacggc acggtctcca ccaccgacga tgttcagaag ctcctcgcac
    18901 gtctcatgaa ggagggcatg gacggcctcg tcctcgacct gcgcggcaac ggcggcggtt
    18961 cccttgagga ggccatccgc ctcacgggtc tctttgtccc ggccggcccc gtcgtccagg
    19021 ccaaggattg gcgcggctcc atcacctggc gcgagtgtga aagccccaag gccttttatg
    19081 aggggcccat gatcgtgctc acggacaaaa ccagcgcctc cgcttcagag attctcgccg
    19141 ccgcgttgca ggactaccgc cgcgccctca tcgtcggaga caaatccacc ttcggcaagg
    19201 gcaccgtgca gaccatcctc cccgtggacc gcttcatgcc cttcttcgcc aaaaaagacc
    19261 gcgccggaaa cctgaaagtc accatccaga agttttaccg catcgccgga ggttccaccc
    19321 agctcaaggg cgtcgaaccc gacctcgtgc tgccttccat ccgggatgtc ctggacatcg
    19381 gcgagtcctc caccgaaaac ccgctgccct acgacacgat cccagcgcgc aaatacaacc
    19441 tcgtctccca gcaccccctg ccggtggccg agctttcatc cagactcgcc gcccgcctcg
    19501 gggccaaccc ggagtttcaa tacatccttg atgaatccaa gcgcctcaag gagcgcattg
    19561 accgcaactc cgtggccatc aacctttccc agcgccagca ggagaccgct gaaaacaaag
    19621 cgcgaaggga aaagcaggag gctgagcgcg cggaacgggc caaagaggtg gcggcccgcc
    19681 tcggagacga aaccttcaag ctctatcatc tcacgctgga caacgtcaac gatcccgagc
    19741 tcgtgcccca gtccgccttc acccgcgagc aaagcaccgg catgaaaatg gccgccaaga
    19801 aggatggcga ggatgatccc aacgacatgt ccaagttccc ctacggcatc gaacccgcgc
    19861 ggctggaaac actccacatt ctgcgtgatt tcctcgaact caccggcaag cagcccacca
    19921 ccgccaaaac ggagggcaag gcgcccgcca actgagttct tcccggcttg ctaaacaaaa
    19981 gggcggagcc gcgggactcc gcccttttta tttttgaatg ggccgggcgg tgagcgcgcg
    20041 tctcacagct tcgtgtagcc caggctctcg cagggcaggt cccacagccg tttgagttcg
    20101 gcgtcgagtt tggtgatgct gaagctcacg cccgccattt cctggcaggt cacaatgttg
    20161 tcgcagatgg tctggtggat cctcaatccg cgcgcctcca ggatggggcg cgccgcgcgc
    20221 agcatgatga gcagctccat gaggtgggtg ctgcccaggc tgttgacgaa gaagacaacc
    20281 tcgtcgcccg cgcccagtgg caggtcgtcg gcaaaaagca ggtcgaggat cttgaccgcc
    20341 agttcgtccg ccgggcacat ggggatgagt cccacgcctt tctcgccgtg gatgcccatg
    20401 cccaggccga tgagatcgtc cgccagctcg aacgtgggcg cgcccgtggc ggggatgctg
    20461 ccgggcttgg tggagacgcc caccgtgcgc gtcatgtcca cggccttttg cgcgatgcgc
    20521 gccagttcgt cgagcgtgtc cacctgggag gcggcggccc ctgccagctt cacgatgggc
    20581 accaggcccg caatgccgcg gcgcttgccc ttttccgtcg gcggggcgga gcagacatcg
    20641 tcattgatga tgacagtttt gacctggatg ccctcctcct cggccagctc ggcgccgatg
    20701 tcgaagttca tcacatcgcc ggcatagttg ccatacaggt agagcacgcc tttgctgcgg
    20761 ttcaccgcct gcgtcgcctc cagcacgatg tccggcggcg gggccgcgaa tatgtctccc
    20821 accgccgcgc catcgcccat gcctttgccg accaggccgt ggtagatcgg ctcatggccc
    20881 gcgccgccgc cgatgagcag ccccggcgcg tccgggcgca ggtcgttcat cacgatggag
    20941 cgcttgcccg cgcgccgcgc cttgccgtcg taggcgagca cgaggccctc gaagagttca
    21001 tcggcgcact tcagcggatc gttgatgatt tttttggcgg tgttcttgct catggtcgga
    21061 ggatgttgaa gttggtgaaa acagaacgcc gggccgcatg ggcgcgtcgc gtccggggga
    21121 cgatgagagc atttcccggc gcgcgtgcaa cgctcattca cacttttgcc cggcacgccc
    21181 tggaggggag gcgggaattt gccggaacgc ggcgcttggc aggcggccat tcttccgcac
    21241 aatgccgcgc ttctggcatg attgaaatcc acggtttctc aacacgcggc cgcagcctgt
    21301 cggggttgcg gcgcgggcta tttttctccc gatgacctgg gtgctctgcg gtctgctggc
    21361 cctcgtggtt tacacccacg cggggtatcc cctgctgatg gccgtgctgg ctcgatggcg
    21421 gcccagggcg ctcgatcccc acgaggatga caaggccggg ttgagcatcg tcgtttgcgc
    21481 gcgggacgcg ggcgcggcca tcaccgcgcg gctggagaat ctgctcgcct gcgtctggga
    21541 aggtgagctg gaaatcctcg tcttttgtga cggctgcgcc gatgacaccg ccgcgcgcgc
    21601 cagggcgctg ggagacgcgc gcgtgcgcgt catcgagtcg cccgaaccgc gcggcaaggc
    21661 ggcggccctc aatgaggcga tcccggcctg ccggttcgcg caggtggtgc tgtgcgatgt
    21721 gcggcagaga ttccatcccg aggctttgcg gcacctcacc cgcccgctgc tggatccgga
    21781 tgtgatggcg gtgagcgggc tgctggagat cgagccctcg gcggcgggcg gcgggcaggg
    21841 cgtggatgtg tattggaaaa tggagcgcag gctgcgcgag tgggaggggc gctatgattc
    21901 ggtcatcggc tgcaccggcg ccatctgcgc catccggcgg gccgccttca cgccgctgga
    21961 gccggacacc atccttgatg atgtggagat tcccatgcgc atggcggtgg ctggcgggcg
    22021 cgtcttcttc gagccgcgcg cccgcgcctt cgacccccag acgctggacc ccgccaggga
    22081 aaaggccagg aagctgcgca cgctcgtggg caattaccag ctcatcgccc gctatccgcg
    22141 ctggctcctg ccgtggcgca accggctctg gtggcagctc atctcccaca aatatctgcg
    22201 cctggccgtg ccctggctgc tcatcggcat cgccgtctgc tctctcatgg ccccgccgct
    22261 gccctgggtg cgggccttgg tcgccgggca ggccctggcg tatgcctgcg cgctcgccgg
    22321 cctgctgatg ccgcgcctgc gctggaaagt cctctccatt cccgccggct tcgtgctgct
    22381 gcagtggagc tgccttcagg ccttcttcgc ctttctcagg caccgtcgcg acctgcgcgc
    22441 cctctggaaa acccccgccc gatcatgatc cgcgccctcg accgctggct gccctcctac
    22501 ctgctacgcg accggcgccg ccccgccgcc acagcggaaa atcccgcgca tgtcttcctg
    22561 gccgtgtgcg accatttcga gcccttccac cacaccgaca aggcgggagc gctgcgggcg
    22621 ctctcggact gggaggcgct ctggccgccc ttggtggagg cgtttcgaga cagttcgggc
    22681 cgcgggccca ggcacagctt tttttacccc gtcgagcaat acgatgagga ggtcatcgag
    22741 cgtctcgccg ccctgtgtga aaaaaccggc agcgaggtcg aggtacacct gcatcatgaa
    22801 aacgacacgg cggagactct caccgcgcag ttgcgcgcgg gcaccgcgaa cctggcccgt
    22861 cacggactgc tctcgcgcgc ggccgacggc gcgccgcgct acgccttcat ccacggcaac
    22921 tgggcgctcg accactcgca cccgcggggc aggcactgcg gcgtctccga tgagctcgcc
    22981 gtgctgcggc gcaccggctg ctacgcggac ttcaccctgc cctccgcgcc cgaccccgcg
    23041 cagacccgga cggtcaactc cgtctattac gcacgcgagg acggcctgcc caagtcccac
    23101 gaccgcggca tcgccgcgcg gtccggcgtg acgggcgggc ttgcagcctc cgatgagcac
    23161 ctgctcctca tccaggggcc gctgggcctg aactggcgcc ggcgcaaatg gggcctgctg
    23221 ccccggctgg aaaatggcga cctcaccggc gcgaacccgc cgaccccggg ccgcttccag
    23281 ctttggacgg aaatctgccc ccatgtcaaa gacgggccgc cctggatctt cgtgaagctg
    23341 cacacgcacg ccggcatccc gcgcaactac cgctccctgc tgggcgacgc cggacgccat
    23401 ttccacgagg gcctcgccgc ccgcgcgcgg caggacccgg cgctgcgctg tcactacgtc
    23461 accgcccgcg agatggtgaa cctcgtccac gccgcc
//
LOCUS       DAOMYJ010000064        23383 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Prosthecobacter sp. isolate AMR_MDS_5629
            NODE_785_length_23383_cov_22.057013, whole genome shotgun sequence.
ACCESSION   DAOMYJ010000064 DAOMYJ010000000
VERSION     DAOMYJ010000064.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867850
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prosthecobacter sp. (wastewater metagenome)
  ORGANISM  Prosthecobacter sp.
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Verrucomicrobiaceae; Prosthecobacter.
REFERENCE   1  (bases 1 to 23383)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 23383)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.30
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:34:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,742
            CDSs (total)                      :: 4,684
            Genes (coding)                    :: 4,676
            CDSs (with protein)               :: 4,676
            Genes (RNA)                       :: 58
            tRNAs                             :: 55
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23383
                     /organism="Prosthecobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_785_length_23383_cov_22.057013"
                     /isolate="AMR_MDS_5629"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1965333"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..982
                     /locus_tag="PLP58_08785"
     CDS             <1..982
                     /locus_tag="PLP58_08785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ08649.1"
                     /translation="ADRLKAADLPKEARERVSGALQEAGKQSEPEDRARTLGQHVLAA
                     DEQMFQGQPQAAGAEFEKLAEKLRGMSLREQGRKELEKLAQQLREAGGNIAGQEESSA
                     MQPMSAPGQQGQQSQSGAPPQVGQAQPGQEGQGAQQPLQPPGLGQQQQSQGQPMSVMQ
                     PGQGQGQGRQMQMAQPGQPGQQGQDGAPMLLAPVPGQKPGDKPPGIIMPGGPPGDSSD
                     QPSIMLAMPGGRDPGAGTADLNAAPTARQDSAGASVVNAQPGAEGPSSTRAVAGGIRQ
                     ETAGNSATQTAVEFLAAEEAALDDTALPATRREQVRRYFNELRKRFDEAP"
     gene            1145..1711
                     /gene="frr"
                     /locus_tag="PLP58_08790"
     CDS             1145..1711
                     /gene="frr"
                     /locus_tag="PLP58_08790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012420357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008135 - translation factor activity, RNA
                     binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HRJ08650.1"
                     /translation="MDPEMTMLECEDAMTRAVEYAIHEFAAVRTGKASPTLVEGLDVH
                     VTSYGAHMKLKQLAMITTPDARLIRIEPFDSSTLHDIDRAIRESRLGLNGSIEGKVIR
                     LPVPVLSQERREQMVKLIRQMAEEAKVRVRAARREAIELLKKGQKDGHITEDDLHRLE
                     KEVQTLTDKNVAVIDQHVASKEKEVMTV"
     gene            1727..2401
                     /locus_tag="PLP58_08795"
     CDS             1727..2401
                     /locus_tag="PLP58_08795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009958846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DapH/DapD/GlmU-related protein"
                     /protein_id="HRJ08651.1"
                     /translation="MHRSEDYLDLRHTRHGILFPAAEPPWAALSRIESYLEFRLTPEI
                     RAQVPQGVSIGEDVFIDEGVTIEPGAVILGPAWIGKNCMIRTGCYIRGNVVVGDNCVL
                     GNSCEFKNCVLFDNCEVPHFNYVGDAVLGYKAHLGAGVVLSNVRLDRSEVVVNGPLRA
                     ESTGLMKFSAIIGDHVEIGCNSVINPGTLIGRRSIIYPLTSFGGILPPDSILKTRQQQ
                     VVVKRV"
     gene            2411..3310
                     /locus_tag="PLP58_08800"
     CDS             2411..3310
                     /locus_tag="PLP58_08800"
                     /inference="COORDINATES: protein motif:HMM:NF013465.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding protein"
                     /protein_id="HRJ08652.1"
                     /translation="MKAPHFLLLTLLIAACKPAAPSADSAGRPRVLSANYPLHYFATR
                     IGGGSVEAAFPAPADEDPAFWQPADDELAAFQNAALILMNGATYSKWAEKASLPASKT
                     VDTSAGFKDKLIEIKDAATHSHGPAGEHSHSGTAFTTWLDFQQAIRQADAVRAALSRL
                     LPDKTADFDAAFHSLKAELESLDARLMKIGARPAKAPLVASHPVYHYWARRYGFNVES
                     VLWEPETVPDEKAMEELKALLARHPAKWMIWEGDPAAESVARLDELGLKSLVFDPCGN
                     TPDEGDFLSVMRANVEALEKALP"
     gene            3359..4051
                     /locus_tag="PLP58_08805"
     CDS             3359..4051
                     /locus_tag="PLP58_08805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009964324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2238 domain-containing protein"
                     /protein_id="HRJ08653.1"
                     /translation="MTHAGLVKKLGAVYALLWLALAIEPRGRQDWLLENVLVLLALPV
                     FWAGCKHRLFSRLSWVMIFAFMCLHALGAHFTYSEVPYDEVWQSLAGFSLDEFFGWRR
                     NQFDRAVHFLYGLLLAYPMREIFLRIANARGFWGYFLPLDLTMSSSLLYELIEWLAVE
                     VLGNDAGMAWLGAQGDVWDAHKDMALAALGAILAMILTVLVNWRCQRDFAREWSESWR
                     VKAPGDGFVEGV"
     gene            complement(4052..8263)
                     /locus_tag="PLP58_08810"
     CDS             complement(4052..8263)
                     /locus_tag="PLP58_08810"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialidase family protein"
                     /protein_id="HRJ08654.1"
                     /translation="MKAILSIFALITPLASAQSLLPANWDPALAGDVVMQRLVKVTAP
                     KVKGTHDAEFVCVGDRAYIVTEANDQRAGESSAWPFIYVTMSIVNLKTLEVEKVIDFA
                     RSEQVFENETLPFGQCFVPRILQKDAKTLRCYFTSQDPGKRQAQMWYIDFDLGRAAFE
                     NRIFRAKLKTAAGSFDMQPQPFYDDAAKQGFRRPARDAGLFIFDSFKKFDGKTYVALN
                     NFPVGQNALSVLHDDLVTFEVIGHYNEPQTQQLSESAVNRLPDGSWMAIVRNDKGNYH
                     FTTSADGKKWTVGTEMPFVKGSNSKPTFDKFGDVYYLGWQDAEVINGDRRTIFNIDIS
                     RDGKAWERKYRFITPHSFQYPTLHEHQGVIWLSVTQGTQKDRIMFGKLESVGQFEPQA
                     GRKRIEWPPVLPKGLAKLNPGEKLFTDREYVIDELPEPVRGLPFLRTSIEKTDVRVTK
                     PGTLFALTPTIRPKAASQEEALQKAGFSKVDVPETQLFPGEINRVSLYRKAVKQGERL
                     QFKKMVLLVLADGAEVRDEDGMSPSVITNPGAEFQDDARGGAMIIGMDRTPKGRIWGC
                     WTGTGDKPDGYFLLATSDDDGATWSKPRVAVGARMEAAQKISGALVGNLWTDPKGRLW
                     HFFDQQLGDPQGRITNWFMRCDNPDAEEPVWSTPVMFAEGCTLNKPTVLKDGAWLLPV
                     SDWHKKTCRVFASTDEGATWKERGSLQFPDWEFDEHMMAELKDGRLWMLARTHGQPHE
                     SFSNDGGKTWSEPKQAATVQNVNARFFLRRLKSGRILLVKNGSPAERLQKRTHMSAWL
                     SEDEGQTWKGGLLLDERNAVSYPDGFEAPDGLIHILYDWNRHTDAEILMAKFREEDVL
                     AGKIVSKDAKLLMLANKATGPKPEKLYNGIELPDQWPPRYREPSDAVMEVPYLKKKPK
                     VIPIDLGRQLFVDDFLIEKTTLKRTFHQAKKFEGNPVFKAETERELGPAREGEKGEEA
                     TTFTGQGGVFYDPAEKLFKMFYVAGWRGPLSLATSPDMKTWTRRGQLLPEGLRWTGPK
                     LATGGSDNCVWLDLNAKSPAERLKYITCWLHVPKEQRPQGFMHSLHVSDGKTFSDAVT
                     TSIAADDYCSFFFNPFREKWCFSIKIGTSRGRSRYYYESDDFLAGADWKKSVFWTCTD
                     KLDLPEPQGRYPGAGEAAQLYSLNAVAYESLMVGMHYIHRGPNNSICEKGKFPKLLDL
                     EIGFSRDGFHWDRPDRRGFIVGSRTEGSWDRAYLHSTAGVFVVLDDQLVFPYMGTSGI
                     APSGHRGMYTGGSIGLATLRRDGFASMDGPGELTTRPVKFKGKHLFVNVNGAVKVEVL
                     DEAGKVLRSSKVASGDQTKLKVEWNDGADLGDLIGKAVKLRFHQTKGSLFAFWVTPDE
                     NGTSGGYVGSGGPQFNGVRDQPPGF"
     gene            complement(8467..9477)
                     /locus_tag="PLP58_08815"
     CDS             complement(8467..9477)
                     /locus_tag="PLP58_08815"
                     /inference="COORDINATES: protein motif:HMM:NF013429.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HRJ08655.1"
                     /translation="MNPPPDFLSRRDALKATIASVGLGSLAFEPAPAAAPSAPDARRG
                     SPKRHDMLKSINLWAFPYPERMTLRECLQLAKDAGFDGIELNYDLDNDLSPKHGTQDY
                     GKIRKMADEIGIQISGLCSFLFWPYPLTSNDPAKRAKGMELAGKIAQCAHDLGVENVL
                     VVPGAVHIPWRDDHEPVPNDVCDQRAREAVGQLARQAEKLKVCLNIENIFFNGYLMTP
                     MEMNTFVDSFGSERVRVHFDTGNISMFQFPEHWARVLGSRTKNVHLKEFTKKGTDFSL
                     ETFRPLLDGTTNWPAVTDALADTGYKSFLTFEYFHPYTHYPEALIWQTSDSMDRILGR
                     KA"
     gene            complement(9587..>10171)
                     /locus_tag="PLP58_08820"
     CDS             complement(9587..>10171)
                     /locus_tag="PLP58_08820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ08656.1"
                     /translation="GQRGQSGQSGQPSQTAQGGQGTQSGPGQEPGQSDAAGQRGAAGP
                     SRQTAQSGSERNRAGGGGGGGDRGGPLFFEEADEQPRQGPITGEGYDRWSDRLRNVEE
                     LLSQPELRNEAARVLDNARAMRIDHERNDAPPQVDHLAMRIIEPLVELRDRVAEELSR
                     REAGNNTVPVDRDPVPPAFRDLVRRYYKELGSGE"
     assembly_gap    10172..10271
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<10272..13463)
                     /locus_tag="PLP58_08825"
     CDS             complement(<10272..13463)
                     /locus_tag="PLP58_08825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ08657.1"
                     /translation="MIEATILQRKLDEVREHLRECLRRRVMARMLLVYGVIAVALVFA
                     VPALGPDGRFLAPLVMLGMALTFFTLNFWVKRRRWSDIEVARMIERRHPDLNALLLTA
                     IEHLEPGAELSEFFQKRLLAEAASKGAVQAWPATVTSRSMRGATAGFVVSALLCASAW
                     VWLLALLPRSFSPEIAAVEKVGPIEKEPEPDPKIEITVSPGDTEIELGSRLIVEAKMQ
                     PVVPDSATLILTDLEGAELDRVPMRLTVDGDAFGGLLTRVKQDALYRVEAGEAVSKTH
                     KITTFVYPALVRADVKITPPAYSGLPAKEIKNTMKATVLEGGEIEFTVTVNKPVKDAE
                     LFGQDKTVLPLKAKAGEPTVLTAVMKPEKSQKWRLHLVDEQERANKNPPWFTVTVQTN
                     QLAKIEVVFPKRDIQVSAIQELPVEAKVSDDIGVTRSGVSFSIAGDHKEVLFQHATTA
                     PAKKQDVKTLLTLENENAAPRQLVSYFFWAEDQGPQGEVRRAMSDMFFADVRHFEDIF
                     REREAPPSMPGEPGQQSQSDKLLDLQKQVVNATWRVIRDTNAGKSMDAAAPDVDVVHE
                     SQGITLDKTKEAMEEAEDAEVKLALTEAWKSMKDALDPLQQAAEEKKRTALNQALGFE
                     QSALEWLHRASSREHLIMRQNQPRQPGQGQRPNENQLSSLELKQEEQRYEEEKMAAEE
                     QSAEQQENLQVLNRLKELARRQEALAEKMKELENQIAQAKTEEEREELENQLKRLQQE
                     QEQLLSDLDDLKERMEKPENASNMAEAREQLDQVREQVMDSAEKLREQQLADAANSAT
                     RAQRELEQMQEDFKQKTARRFTEEMRQLRQQAREVAEQQKQISESLENQKTPDPGQST
                     STLEKMLDGSQVARKIEEQSGKVGELLENMRRISEQAEGNNPLLHRRLYESVRDAQTS
                     GLEENLEEARLQSRYGQRAGAQDAERKAAAAVEKLQQGVDKAAESVLGSETDALRMAR
                     SELDKLIKEAESEAGQAGRTDPSDQTDQTAQRGQSGEEDQPQGQRGQGSQPSQTAQSG
                     QPGEEGQPQGQRGQGVQPSQTAQSGQPGEEGQPQ"
     gene            13655..14605
                     /locus_tag="PLP58_08830"
     CDS             13655..14605
                     /locus_tag="PLP58_08830"
                     /inference="COORDINATES: protein motif:HMM:NF018038.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ThuA domain-containing protein"
                     /protein_id="HRJ08658.1"
                     /translation="MKPLIFALAALGLALLHSQCSPDKAGAADAPGGKKLVVMIAGSP
                     SHGPGQHEHNAGILLLKKCLEQGAGTQVEIKHHLNGGWPAADELAKADTVVIYADGGG
                     RHPALQEDRLAQLDKEMKRGCGFATIHYAVEPTIEKGQKEFLDWMGGCFEINWSVNPH
                     WQAEFKEFPAHPVASGVKPFGTLDEWYFFMRFREGMKGVTPILSSAAPESTMSRPDGH
                     HSGNPHVRASVARGDKQHVAWASENAGGGRGFGFTGGHFHQGWANDDQRKLVLNAILW
                     TAKGEVPAAGVASTVTDEDMKANLDLKPGQGLPEKAKKAK"
     gene            complement(14624..15514)
                     /locus_tag="PLP58_08835"
     CDS             complement(14624..15514)
                     /locus_tag="PLP58_08835"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HRJ08659.1"
                     /translation="MNTTHDTAVPMVNLRRGSAWMLLSLAGFTGMALLVRHLGAERGV
                     SAWWALFFRAAAGMAVVWAVFAPGGRVSFRRALRGRLLVTRGVLGALGTAAYYVTLPV
                     LGAGKATLIGNTWAVWAALFAAFMLGEKLAVRKLAGIVLAVAGVALLTGLQGGDLSRV
                     GGYDAIALAGAFAAAFVVVVIRQLTLTETSGTIFASQCIYAGALALPFLFSLPLPGGL
                     DILLLTAAALLAALGQLAMTEGFRYLPVSTGGAFQILLPLVLTVAGVLFFDETFTLTQ
                     AAGAACILSGCHLTVAERKS"
     gene            15591..16907
                     /locus_tag="PLP58_08840"
     CDS             15591..16907
                     /locus_tag="PLP58_08840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009959968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ08660.1"
                     /translation="MRAALLIFLVCTTLHAQQPPYDAFPAADPPYYRVRYEPSRTEGG
                     LIFGVNYTLWIPPGVKTLRGVIVHQHGCGEGSCKSGLTGAYDLHWQALAKKHDCALMA
                     PAYEQPDKADCQMWCDPRYGSSAAFQKALSDLGAKSGHPELAEVPWALWGHSGGGHWA
                     GGMALLHPERVAAAWLRSGVPLLTANPDRATIKPHTLDDAVPAVPMMCNPGTKEGVTV
                     KEGRFAGVWAANETFFKTLRAKGGLVGVAVDPLTSHECGNQRYLAIPWLDTCLSARLP
                     DKAGEPLRKMNPDKAWLAPLLGTGAAPAEKFTGDTQSAVWLPDGGTAKLWRHYITDTV
                     VPDTTPPPAPVNLQVNGRTLTWECEADLDSGLAGFLIERDGVIITPAPIQAKNPFGRP
                     VFQGLQYSDTPAMPLVEMSYTDKDATPGGSHRYRVIALNTAGLRSE"
     gene            complement(16870..17829)
                     /locus_tag="PLP58_08845"
     CDS             complement(16870..17829)
                     /locus_tag="PLP58_08845"
                     /inference="COORDINATES: protein motif:HMM:NF016228.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar nucleotide-binding protein"
                     /protein_id="HRJ08661.1"
                     /translation="MSTARRILILGGTGRLGGVLAREWQGRHEVMAPGRRLLDASQPA
                     ALARELRALEFDTLVNTAGLTSPDTCEERPDDAAALNAAAPAVLAAECARRRARLIHL
                     STDYVFDGGGGALLDEDAPARPVNVYGRTKLAGEKAVLAACPEALVARVSWLFGPRGG
                     DVPATVLERARSGQPLDFIEDKWSAPTGIADLTAWLERFVTDLRPQSGVLHLCHTGVA
                     TWRDYAQVTLDLAHAAGLIERPCHTIGRRLREFSHFKAARPPFTVMSNARLSEVLGAP
                     IPAWQEALERHLAGIARHRGDEWDAGGGRNAVTPISDPPCSKR"
     gene            18058..19803
                     /locus_tag="PLP58_08850"
     CDS             18058..19803
                     /locus_tag="PLP58_08850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ08662.1"
                     /translation="MPLASARERTYFCLVHDPRSPVEYYANATGGGRGLFGGTDEVAV
                     TRAGEAAWRVSVRATKGEWAYGGFINAVTRAVEDQVMVNPNALWHPMILKEFQPRLKS
                     IRVELAGARSPAGRADLHLRLELKGWDKNGAQVPAQAFAADRATLLNGPHPKKLSFPV
                     DAMTGPAGAMIVMLDNAAAGDEAEIGAIEWEVALGETPLPRRAALLGLGALLANWDSS
                     SGMAQDRGSFPAGIYENLTATAKLAKIIALAAREGIVDETAGKAAIEKIAATLLDVVP
                     RGPQPSNRLWPHFTRNGGTARHEGSEWSSGDTAYAVADLALALALTGDAQKRLPQLME
                     MLRGINWRMLHDRGFYRHGYDRDGKIIPHRWQGFGVETLGVNLAALAGGGPPAEMLPP
                     PTDDGSGFNMHAAYPLPFAAVDCHGNDWRRLRLEEIRAQTGWHRQTARANTRLADAHL
                     FGLSAAETPDGQNYMAYGIGGRLDGARDGGGRIVTPHYCGMVAALAPEDAARMVESLE
                     ARGWLTPLNSADSVEIDPVTGRETVNWMKGSWNLALFTEGWLFANPDILSAANEALRS
                     IPEFARAWDLCFPPR"
     gene            19894..22224
                     /gene="murC"
                     /locus_tag="PLP58_08855"
     CDS             19894..22224
                     /gene="murC"
                     /locus_tag="PLP58_08855"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009962545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008763 - UDP-N-acetylmuramate-L-alanine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HRJ08663.1"
                     /translation="MTHAVLSLLRESRHPMRINLIGVAGSGMSGLASLLLALGHKVSG
                     SDKVTTLETARLQKLGLQFFSPHSEQEVAECDMVIYSSAIKPGNVAYEAAYKQGIPLV
                     RRAEALAAVMANKKGIIVGGTHGKTTTSALTAHVLRQGGLKPSHYVGAEIPILGSNAH
                     WDTGGELFVAEGDESDGTLVHFHPQHAIILNIEAEHLDFYDGIEAIKAVFRQLLAQTP
                     GWKVYCAEDPVAREVCGAAENAVGYGWSREMDFSAEIVEMKPDHTDFIVWQKDERLGE
                     ATLGIPGRHNVSNALAAIALATRLGVPFENIRHALKSFRGARRRFEIRHGGARFTVVD
                     DYGHHPSEIAATLATARGLKPGRIVCLFQPHRFSRTQLLKKEFGASFDDVDELFVTDV
                     YAASEKPLPGVSGGTIVEAVEAHKAAAGGVKTPVCHSTPALLQARDKAGNALRPGDLL
                     ITLGAGNVHEVGRCIARDLPVLEKLWELLEAHGGGAARLYEPMNRHTTYLIGGQAQFW
                     VEPRTIGGFAEVVRYLRSASVPIRVIGRGSNLLVKDGGIRGAVIHPAKGEFEEVRVEG
                     ETLIAGAGARLKKIASTARNAGLGGFEWMEGVPGNLGGAIRMNAGAMGTETFDQIVSV
                     RFIDVDGALREKPLAEITHHYRSVPEFEERYIVSAVLKGAPAAREEIDERLAASHHKR
                     RTTQPVGASAGCVFKNPELCGAGKLVDDLGLKGRGVGRAVVSPVHGNFIVNTGGATAR
                     EVLDLVAEIQETAQRERGVSLELEVKVIGEDHPLPL"
     gene            22366..>23383
                     /locus_tag="PLP58_08860"
     CDS             22366..>23383
                     /locus_tag="PLP58_08860"
                     /inference="COORDINATES: protein motif:HMM:NF015155.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HRJ08664.1"
                     /translation="MKSPWLIPALLIPLVILGWLRLRFETDILATLPADVPEVRAVKL
                     LRAGFTGGTNLVIGLEAEDDILAEEAANSLGRRLEKESALASEVRWARPVEEQAETGS
                     ALLAWMLQNAEPAEWGKLRARLEGDGAKAQVAKSFHTVGHSLDAEKVQRASYDPLGLM
                     DALSLDDLQSMGDSSFGLASEDGRFRLLLVTPMAEVGNYKDADKWLQRVREIVAAWKG
                     EEGFEECAVRLTGEPAFQSEVGMGIEEDMSGTMGLTAGLIGLLFWLMFRRLKLLLWIQ
                     LLLALSMVTAIGIGGLLVGRLSMMSLGFAAIVMGIIVDYAVLILQEARQHPQLPAGPL
                     RRLAA"
BASE COUNT         4072 a         7615 c         7391 g         4205 t
ORIGIN      
        1 cgccgaccgg ctcaaggccg ccgatttgcc caaggaggcg cgcgagcgcg tgagcggcgc
       61 gctccaggag gccgggaaac agtccgagcc ggaggaccga gcgcgcaccc tcggccagca
      121 tgtgctggcc gcggacgagc agatgttcca ggggcagcct caagccgcag gtgccgagtt
      181 tgaaaaactg gctgagaaac tgcgtggcat gtccctgcgc gagcaagggc gcaaggaact
      241 cgaaaaactc gcccagcagc tccgcgaagc cggcggcaac atcgccggcc aggaggaatc
      301 cagcgccatg cagcccatga gcgcccccgg ccagcaaggg cagcagagtc agagcggcgc
      361 ccccccccag gtcggccagg cgcagccggg ccaggaaggc cagggtgctc agcagcccct
      421 gcaacctccc ggactcggcc agcagcagca aagccagggc cagccaatga gtgtcatgca
      481 gccaggtcaa ggccagggcc agggccgcca gatgcaaatg gcgcagcccg gccagccggg
      541 acagcaagga caggatggcg cgccgatgct cctcgcccct gtccccggcc aaaagcctgg
      601 ggacaagccg ccggggatca tcatgcccgg cggccctccc ggtgactcgt ccgatcagcc
      661 gtccatcatg ctcgccatgc ctggaggccg cgaccccggc gccggcaccg ccgatctcaa
      721 tgcagccccc accgcccggc aggacagcgc gggcgcatcc gtcgtcaacg cccagcctgg
      781 agccgagggc ccctccagca cgcgcgccgt ggccggaggc atccgccagg aaactgccgg
      841 gaactccgcc actcaaaccg cggtggaatt cctcgccgcc gaggaggccg cgcttgatga
      901 caccgctctc cccgccaccc gccgcgagca ggtgcgccgc tacttcaacg agctgcgcaa
      961 gcgttttgac gaagccccct gaaccagaaa aacgcaggca agccgcgcgc tttgacacgc
     1021 tggcgggcaa ggcgcggcac ccgcgcactc agggtcaaat tccagggtgg acatttcccc
     1081 cccgcgctgg caacctcacc gcccaccggc aacgagcccg aatcccagac ccgtttgtta
     1141 ctttatggac cctgaaatga ccatgcttga gtgcgaggac gcgatgacac gcgccgtcga
     1201 gtacgccatc cacgaattcg ccgctgtgcg caccggcaag gcctccccca ctctcgtcga
     1261 ggggcttgac gtgcatgtca ccagctacgg cgcgcacatg aagctcaagc agctcgcgat
     1321 gatcaccacg ccggacgcgc gcctcatccg catcgagccc tttgactcct ccacactcca
     1381 tgacattgac cgtgccatcc gcgagtcccg gctcggactc aacggcagca tcgaaggcaa
     1441 ggtcatccgc ctgcccgtgc ccgtgctctc ccaggagcgc cgcgagcaga tggtcaagct
     1501 catccgccag atggccgagg aggccaaggt gcgcgtgcgc gcggcccgca gggaggccat
     1561 cgaacttctg aaaaaaggcc agaaggacgg ccacatcacc gaggacgacc tgcaccgcct
     1621 ggagaaggag gtgcaaaccc tcaccgataa aaatgtcgcc gtcatagacc agcatgtcgc
     1681 cagcaaggaa aaggaagtga tgaccgtctg agacggcccc ctcgccatgc accgctctga
     1741 agactacctc gacctgcggc acactcggca cggcatcctg tttccagccg ccgagccgcc
     1801 ctgggccgcg ctctcgcgca tcgagtccta ccttgagttc cgcctcaccc ccgaaatccg
     1861 cgcccaggtc ccccagggcg tcagcattgg cgaggatgtg ttcattgacg agggcgtcac
     1921 catcgagccg ggcgcggtca tcctcggtcc cgcatggatc ggcaaaaact gcatgatccg
     1981 caccggatgc tacatccgcg gcaacgtggt cgtcggcgac aactgcgtgc tgggaaactc
     2041 ctgcgagttc aaaaactgcg tgctctttga caactgcgag gtgccgcact tcaactatgt
     2101 gggggacgcg gtcctcggct acaaggccca tctcggagcc ggagtcgtgc tttccaacgt
     2161 ccgtctcgac cgctccgagg tcgtcgtcaa cggccccctg cgcgccgaat caaccggcct
     2221 catgaaattc agcgccatca tcggcgacca tgtcgagatc ggctgcaaca gcgtcatcaa
     2281 cccgggcacc ctcatcggcc gccgcagcat catttacccc ctcaccagct tcggcggcat
     2341 cctcccgccc gacagcatcc tcaaaacccg gcagcaacag gtcgtggtga aaagagtctg
     2401 agcatccgcc atgaaagcac cgcatttcct tctccttacc ctgctcatcg ccgcctgcaa
     2461 acctgccgcc ccgtccgctg actccgctgg caggccgcgc gtgctctcgg cgaactatcc
     2521 tctgcactac ttcgccacgc gcatcggcgg agggtccgtc gaggccgcct tcccggcgcc
     2581 cgcggatgag gacccggctt tctggcagcc tgccgacgac gaactggccg cctttcaaaa
     2641 cgccgcccta atcctcatga acggcgccac ttattcgaag tgggccgaaa aagccagcct
     2701 gcccgcctcg aagaccgtgg acacctccgc gggcttcaag gacaagctca tcgagatcaa
     2761 agacgccgcc acgcacagcc acggcccggc gggcgagcac agccacagcg gcaccgcctt
     2821 caccacatgg ctggattttc aacaagccat ccggcaggcc gacgccgtgc gcgccgctct
     2881 ctcgcgcctg ctgccggaca agaccgcgga tttcgacgcc gctttccaca gcctcaaagc
     2941 cgaactggag tcgctggatg cgcgtctgat gaaaatcggc gcgcgtcccg ccaaagctcc
     3001 tctcgtggcc tcgcatccgg tctatcacta ctgggcgcgg cgttacggtt tcaatgtcga
     3061 gtccgtgctc tgggaacccg agactgtgcc ggatgaaaag gcgatggagg agttgaaagc
     3121 gctgctcgcc agacacccgg ccaaatggat gatctgggaa ggcgatcctg cagccgaaag
     3181 cgtggcaagg ctggacgagc tgggcttgaa gtccctcgtc ttcgacccct gcggcaacac
     3241 gcctgatgaa ggcgatttcc tcagcgtcat gcgggcgaat gtggaggcgc tggaaaaagc
     3301 gcttccttga acggccgcgc gctgcgtgaa tcgcgctccc gtccgcgttt gaaagcgcat
     3361 gactcatgcg ggcctggtga aaaagctcgg cgcggtttac gcgctgctct ggctcgcgct
     3421 cgccatcgaa ccacgcggca ggcaggactg gctgctggaa aacgtgcttg tgctgctggc
     3481 tctgccggtc ttttgggcgg gttgcaagca ccggcttttt tcgcgcctgt cctgggtgat
     3541 gattttcgcc ttcatgtgcc tgcacgcgct tggcgcgcac ttcacctact ccgaggtgcc
     3601 ctatgacgag gtttggcaat cgctcgcggg gttcagtctt gacgagtttt tcggctggcg
     3661 gcgcaaccag ttcgaccgcg ccgtccactt cctctacggc ctgctgctgg cctatcccat
     3721 gcgggagata tttttgcgca ttgccaacgc gcgcggcttc tggggatact tcctgccgct
     3781 cgatctgacc atgtcctcct cgctgctcta cgaactcatc gaatggctcg ccgtggaagt
     3841 gctcggaaat gacgccggca tggcctggct tggcgcgcag ggtgatgtgt gggacgcgca
     3901 caaggacatg gccctcgccg cactcggcgc catcctcgcg atgatcctca ccgtcctcgt
     3961 caactggcgc tgccagcgcg acttcgcccg cgagtggtcc gaaagctggc gggtgaaagc
     4021 gcccggggat ggctttgtgg aaggcgtctg atcagaagcc gggcggctgg tcacgcacgc
     4081 cattgaactg cggcccgccc gagccgacgt agccgccgct ggtgccgttt tcatccggcg
     4141 tgacccagaa cgcaaacaac gagccctttg tctgatggaa gcgcaatttc accgccttcc
     4201 caatcaggtc accaaggtca gcgccgtcat tccattccac cttgagcttc gtttgatccc
     4261 cggaagccac tttggagctt cgcagcacct tccccgcctc atcgagcact tccactttca
     4321 ccgcgccgtt cacattcacg aagagatgct tgcctttgaa cttcaccgga cgcgtcgtca
     4381 gctctccagg gccatccatt gaggcaaaac catcgcgacg cagcgtggcg aggccgatgc
     4441 tgccgccggt gtacatgccg cgatgaccgc tcggcgcgat gccgctggtg cccatgtagg
     4501 gaaagacgag ttgatcatcg agcacgacaa acactcccgc cgtgctgtga agatacgcac
     4561 gatcccaaga accctccgtg cgcgagccga cgatgaaacc gcggcgatcc ggtcgatccc
     4621 agtgaaagcc atcgcgactg aagccaatct cgagatcgag cagcttcgga aacttgccct
     4681 tctcgcagat gctgttgttc gggccgcgat ggatgtagtg catgccgacc atgaggcttt
     4741 cataggccac ggcattcagg ctgtagagct gcgcagcctc gcccgcgccg ggatagcggc
     4801 cctgcggctc ggggagatca agtttgtccg tgcaggtcca aaacacgctc ttcttccagt
     4861 cagcaccggc gaggaaatca tcgctctcgt agtagtagcg actgcgaccg cgtgaggtgc
     4921 cgatcttgat gctgaagcac cacttctcac gaaacggatt gaagaagaat gagcagtaat
     4981 catccgccgc gatgctcgtc gtcaccgcat cggagaacgt tttgccatcg gagacatgca
     5041 gcgagtgcat gaagccctgt gggcgctgct ccttcggcac atgcagccag caggtgatgt
     5101 atttgaggcg ctctgcaggt gatttggcat tcaaatcgag ccaaacgcag ttgtcggagc
     5161 caccggtggc gagcttcggt ccggtccagc gcagtccttc cggcaaaagc tgcccgcgac
     5221 gcgtccatgt tttcatgtcc ggactcgtcg caagcgatag cggccctcgc caaccggcga
     5281 cgtagaacat cttgaagagc ttttcggccg gatcgtaaaa cacaccgccc tggccggtga
     5341 aggtggtcgc ttcctcgccc ttttcgcctt ctctcgccgg gccgagttca cgctcggttt
     5401 cggctttgaa gactggattg ccctcaaact tcttcgcctg atgaaaagtg cgtttgagcg
     5461 tggttttctc gatgaggaag tcatcgacga agagctgcct gccgaggtcg atggggatga
     5521 ctttgggctt tttcttcaaa tacggcactt ccatcaccgc gtcgctcggc tcgcggtaac
     5581 gcggcggcca ttgatcgggc agttcgatgc cgttgtagag tttttcgggc ttcgggccgg
     5641 tggccttgtt cgccagcatg agcagcttcg cgtccttcga cacgattttg cccgcgagca
     5701 cgtcctcctc gcgaaacttc gccatcaata tctccgcgtc ggtgtgccgg ttccaatcat
     5761 ataggatgtg gatgagccca tcgggcgctt cgaagccatc gggatacgac accgcgttcc
     5821 gttcatcgag cagcagaccg cctttccacg tctggccttc atcctcggaa agccacgcgc
     5881 tcatgtgcgt gcgcttttgc agtcgctcgg caggcgagcc attcttcacc agcaaaatgc
     5941 ggcctgattt gagccgacgc aggaagaagc gcgcattcac attttgcact gtcgcagctt
     6001 gtttcggctc gctccacgtt ttgccgccgt cattcgaaaa actctcgtgc ggctgaccat
     6061 gcgtgcgagc gagcatccac aggcgtccat ccttcaactc ggccatcatg tgctcgtcga
     6121 actcccaatc cgggaactgc aggctgccgc gttccttcca cgtcgcgcct tcatcggtcg
     6181 aggcaaacac acggcacgtt ttcttgtgcc aatccgacac cggcagcagc cacgcgccat
     6241 ctttgagcac cgtcggcttg ttcagcgtgc aaccttccgc aaacatcacc ggcgtgctcc
     6301 ataccggctc ctccgcatcg ggattatcgc agcgcatgaa ccagttcgtg atgcgtccct
     6361 gaggatcgcc gagctgctgg tcgaaaaaat gccacaaacg ccccttcgga tcggtccaca
     6421 gattgcccac cagcgccccg ctgatcttct gcgccgcctc catgcgtgct ccgacggcca
     6481 cgcgaggctt cgaccacgtc gcgccgtcat catcgctggt cgcgagcagg aagtagccat
     6541 ccggcttgtc ccccgtgccc gtccagcagc cccagatgcg ccccttcggc gtgcgatcca
     6601 tgccaatgat catcgcgccg ccacgtgcgt cgtcttgaaa ctccgcgccg ggattcgtga
     6661 tgaccgaagg cgacatccca tcctcatccc gaacctctgc cccatccgcg agcaccagta
     6721 gcaccatctt cttgaactga agccgctcac cttgcttcac ggctttgcgg taaaggctca
     6781 cgcgattgat ttcgccgggg aaaagctgtg tctcaggcac atcgactttg ctgaagcctg
     6841 ctttttgcag cgcttcttcc tgactcgcgg ctttggggcg aatcgtcggt gtcagtgcga
     6901 agagcgtgcc gggcttggtg actcgcacat cggtcttttc gatgctcgtg cggaggaatg
     6961 gcaggccgcg cacgggctca ggcagttcgt cgatcacata ctcgcgatcg gtgaagagct
     7021 tctcgcctgg attcagcttc gccagtccct tcggcagaac tggcggccat tcgatgcgct
     7081 tgcggcctgc ttgcggctcg aactggccca cggactccag tttgccgaac atgatgcggt
     7141 ctttttgcgt gccttgggtg acactgagcc agatcacgcc ctgatgctca tgcagcgtgg
     7201 gatattgaaa tgaatgcggc gtgatgaagc ggtatttgcg ctcccaggct ttgccatcgc
     7261 gtgagatgtc gatgttgaag atggtgcggc ggtcgccgtt gatcacctcg gcgtcctgcc
     7321 agccgaggta atagacatcg ccaaacttgt cgaaggtcgg cttcgagttt gagcccttca
     7381 cgaagggcat ctcggtgccg acagtccatt ttttgccatc agcgctggtg gtgaagtggt
     7441 agttgccctt gtcgttgcga acgatggcca tccaggagcc gtcaggcagg cggttcacgg
     7501 cggattcgct gagctgctgc gtttgcggct cgttgtaatg cccgatcact tcaaaggtga
     7561 cgaggtcgtc gtgcagcacc gagagcgcgt tttgcccgac ggggaagttg ttcagcgcga
     7621 cgtaggtctt gccatcgaac ttcttgaacg aatcgaagat gaacaggccc gcgtccctgg
     7681 ctggacggcg aaacccctgc tttgccgcgt catcgtagaa aggctgcggc tgcatgtcga
     7741 agctgcctgc cgctgtcttg agtttcgcgc ggaaaatgcg gttctcgaag gccgcgcgtc
     7801 ccaggtcgaa gtcgatatac cacatctgag cctgtcgctt gccggggtcc tggcttgtga
     7861 aatagcagcg caacgtcttc gcgtcttttt gaaggatgcg cggcacaaaa cactgcccga
     7921 acggcaacgt ctcgttttca aaaacctgct cactgcgggc aaagtcgatg accttttcca
     7981 cctccagcgt cttcaggttc acaatcgaca tcgtgacata gatgaacggc cacgcggagc
     8041 tctccccagc gcgttggtca ttcgcctccg tgacgatgta agcgcggtcg ccgacgcaga
     8101 cgaactccgc atcgtgagtg cccttcacct tcggcgcggt gactttgaca agccgctgca
     8161 tcaccacatc acccgcgagt gcgggatccc aattggcagg gaggaggctt tgagccgacg
     8221 ccagcggcgt gatcagggca aagatggata gaatggcttt catcgtggca atggagtacc
     8281 ttctgaacgc cagacatgga gcgcatcagg cagggacatg gtgtttccga gtgatatgtt
     8341 aaccgcgcct gaaaaacgaa gcgattcagc ggactccaac ccctgcgaaa cccaacgcct
     8401 tcataatcct tcacggtgac gaaatcggtg acgccttggg cgtgcatgat accagccggg
     8461 catggctcac gccttgcggc cgaggatgcg gtccatcgag tcggaggtct gccagatgag
     8521 cgcctccggg tagtgggtgt agggatggaa gtactcgaag gtgaggaagc tcttgtagcc
     8581 ggtgtccgcg agcgcgtcgg tgacggcggg ccagttcgtg gtgccgtcga gcagcgggcg
     8641 gaaggtttcc aggctgaagt cggtgccttt tttggtgaac tccttgaggt ggacgttctt
     8701 ggtgcggctg ccgagcaccc tggcccagtg ctcggggaac tggaacatgc tgatgttgcc
     8761 cgtgtcgaaa tgcacccgca cacgctcgct gccaaagctg tccacgaagg tgttcatctc
     8821 catcggggtc atgaggtagc cgttgaaaaa gatgttttcg atgttgaggc agaccttgag
     8881 cttttccgcc tgcctggcga gctgccccac ggcctcgcgg gcgcgctggt cgcacacgtc
     8941 gttgggtacg ggttcgtgat cgtcacgcca tgggatgtgg accgcgccgg gcacgacgag
     9001 gacgttctcc acgccgaggt catgggcgca ttgggcgatt tttccggcca gctccatgcc
     9061 cttggcgcgt ttggcggggt cgttgctggt gagcggatac ggccagaaaa gaaaggagca
     9121 cagcccgctg atctggatgc cgatctcatc ggccattttg cggattttcc catagtcctg
     9181 cgtgccgtgt ttgggggaga ggtcgttgtc gagatcgtag ttcagctcga tgccgtcgaa
     9241 gcccgcgtct ttggcgagtt gcaggcactc gcgcagcgtc atgcgctctg gatagggaaa
     9301 ggcccagaga ttgatggact tcagcatgtc atgacgcttc ggggagccgc ggcgggcgtc
     9361 cggcgcggag ggtgcggcgg cgggggcagg ctcaaaggcc agggagccga ggcccacgct
     9421 ggcgatggtg gctttcagcg cgtcacgacg ggagagaaaa tcaggcggcg ggttcatacg
     9481 acgatggaat caaagcggcg aaaaggtagc aagcctgttt tgccatgacg ggattcttca
     9541 gggcggtggt gcgtccagcc ccctcagttc gtgtccgcat cgaagatcat tcgccgctgc
     9601 caagctcctt gtaatagcgg cgcacgaggt cgcggaaggc gggcgggacg ggatctcggt
     9661 ccacggggac ggtgttgttg ccagcctcgc gcctggaaag ctcctcggcc acgcggtctc
     9721 gcagctcgac gagtggttcg atgatgcgca tcgccaagtg gtccacctga ggcggcgcgt
     9781 cgttgcgctc gtggtctatg cgcatggcgc gcgcgttgtc gagcacgcgc gcggcttcat
     9841 tgcgcagctc gggctggctg aggagctcct ccacattgcg caggcggtcg ctccagcggt
     9901 catagccttc accggtgatg gggccttggc ggggctgctc atcggcttct tcgaaaaaga
     9961 gcgggccgcc tctgtcgccg cccccgcctc cacctccggc gcggtttctt tcgctgccgg
    10021 actgggcggt ttggcggctt ggacccgccg cgcctctttg gccggcggcg tctgactggc
    10081 cgggttcctg tcctggtccg gactgtgtgc cctggccgcc ttgggctgtt tgagaaggct
    10141 gacccgattg accagactga ccgcgctggc cnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    10201 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    10261 nnnnnnnnnn nttgcggctg accttcctca cccggctggc cgctttgggc tgtttggctg
    10321 ggctggacgc cctggccgcg ctggccttgc ggctgacctt cctcacccgg ctggccgctt
    10381 tgggctgttt ggctgggctg gctgccctgg ccgcgctggc cttgcggctg atcttcctcg
    10441 ccggactggc cgcgctgggc tgtctgatcg gtctgatctg acggatcagt ccgcccagcc
    10501 tgcccggcct cgctctccgc ctccttgata agtttgtcga gttcagagcg cgccatgcgc
    10561 agggcgtcgg tctcgctgcc gagcacgctt tcggcggctt tatccacgcc ttgctggagc
    10621 ttctcgacgg cggcggcggc tttgcgctcg gcgtcctggg ctccggctcg ctggccgtag
    10681 cggctttgca ggcgggcttc ctccaggttt tcctccaggc cgctggtctg ggcgtcgcgc
    10741 acggattcat agaggcggcg gtgcagcagg gggttgttcc cttcggcctg ctcgctgatg
    10801 cggcgcatgt tttcgagcag ctcgcccacc ttgccgctct gctcctcgat tttccgcgcc
    10861 acctggctgc catcgagcat tttttccaag gtggaggtgc tctggccggg gtctggggtc
    10921 ttttgatttt cgagcgactc gctgatctgc ttttgctgct cggccacctc gcgcgcttgc
    10981 tggcggagtt ggcgcatctc ctcggtgaaa cgcctggcgg tcttttgttt gaagtcctcc
    11041 tgcatctgct ccagctcgcg ctgggcacgg gtggcggagt ttgcggcatc ggcgagctgc
    11101 tgctcgcgca gtttctcggc ggagtccatc acctgctcgc gcacctggtc gagctgctcg
    11161 cgcgcctcgg ccatgttgga ggcgttctcg ggcttctcca tccgctcctt gaggtcgtcg
    11221 aggtcgctca aaagctgctc ctgctcctgc tgcaaccgct tgagttgatt ctccaattcc
    11281 tcgcgctcct cctcggtctt cgcctgggcg atctggtttt ccagctcctt catcttctcc
    11341 gccagcgcct cctggcggcg ggcgagttcc ttgagccggt tgagcacctg caggttttcc
    11401 tgttgctcgg cgctctgctc ctcggcggcc attttttcct cctcgtaacg ctgctcctcc
    11461 tgcttcagct ccaggctgct gagctggttt tcgttcggcc gctggccctg cccaggctgg
    11521 cgcggctggt tctggcgcat gatgaggtgc tcgcggctgc tggcgcggtg cagccactcg
    11581 agcgcgcttt gttcaaagcc cagcgcttga ttcaaggcgg tgcgtttctt ttcctcggcg
    11641 gcctgttgga gcggatcaag cgcgtccttc atgctcttcc atgcctcggt gagcgcgagt
    11701 ttcacctcgg cgtcctcggc ctcctccatg gcctccttgg tcttgtccaa agtgatgccc
    11761 tgggactcat gcaccacatc cacatccggc gcggcggcgt ccatgctctt gccggcgttc
    11821 gtgtcgcgaa tgacgcgcca agtggcgttg accacctgct tttgcagatc gagcagcttg
    11881 tcgctctgcg actgctggcc tggctcgccg ggcatggagg gcggggcctc gcgctcgcgg
    11941 aagatgtcct caaaatggcg cacgtcggca aagaacatgt cgctcatggc gcggcgcacc
    12001 tcaccctgcg gtccctggtc ctcggcccag aagaaataac tgacgagctg gcgcggcgcg
    12061 gcattttcgt tctccagagt gagcagggtc ttcacgtcct gcttcttcgc gggcgcggtg
    12121 gtggcgtgct gaaagagcac ctccttgtgg tcgcccgcga tgctgaagga cacaccactg
    12181 cgggtgactc cgatgtcatc cgacaccttt gcctccacgg gcagctcttg gatggcggag
    12241 acctggatgt cccgcttcgg aaagacgacc tcgatcttgg cgagctggtt cgtctgcacg
    12301 gtgacggtga accagggcgg gtttttgttc gcgcgctcct gctcatccac gagatgcagc
    12361 cgccatttct gcgacttctc cggcttcatc acggcggtga gcacggtggg ttcaccggct
    12421 ttggctttga gtggcagcac ggttttgtcc tggccgaaaa gctcggcgtc cttcacgggc
    12481 ttgttgacgg tgacggtgaa ctcgatttcg ccgccttcga gcacggtggc cttcatcgtg
    12541 tttttgatct ccttcgcagg caggccgctg taagccggcg gggtgatttt cacatcggca
    12601 cgcactaggg cgggatagac aaaggtggtg atcttgtggg tcttcgacac cgcctcgccc
    12661 gcctccacgc gatacagcgc gtcctgcttc acgcgcgtga gcaaaccgcc aaaagcatcg
    12721 ccatccacgg tgaggcgcat gggcacgcgg tccagttccg cgccttcgag gtcggtgaga
    12781 atgagcgtgg cgctgtccgg caccacgggt tgcattttcg cctcgacgat gaggcggctg
    12841 ccaagctcga tctccgtgtc gccgggactg acggtgatct cgattttcgg gtcgggctcg
    12901 ggctcttttt cgattggccc caccttttcc acagccgcga tctccggcga aaaactgcgc
    12961 ggcagcaaag ccagcagcca gacccaggcg gaggcgcaaa gaagcgccga caccacaaac
    13021 cccgccgtgg ctccgcgcat ggagcggctg gtgacggtgg ccggccaggc ctgcaccgcg
    13081 cctttggatg ccgcctcggc gagcaggcgc ttttgaaaaa actcggacaa ctccgcgccc
    13141 ggctccagat gctcgatggc ggtgagcagc agcgcgttga gatcggggtg gcggcgctcg
    13201 atcatgcgcg ccacttcgat gtcggaccag cggcggcgtt tgacccagaa attgagcgtg
    13261 aaaaacgtca acgccatgcc gagcatcacg agcggcgcga gaaaacgccc gtccggtccc
    13321 agcgcgggca ccgcaaagac cagcgcgacg gcgatgacgc catagaccag gagcatgcgc
    13381 gccatgacgc ggcggcgaag gcactcgcgc agatgctcgc gcacctcgtc gagttttcgt
    13441 tggaggatgg tggcttcgat catgggagag gagagttgaa gggcgctgcc gccagcggat
    13501 tagaacgctg gaaccgtgcc tttctgcctg agtttccaga gaaaggaccg tctctgaaaa
    13561 aagaaagccc gcctccatga acggatcggc ggcgtaccgc gaagcagctg aaatgccccc
    13621 tgtcccgccc gcgttagcat ccacccattc cctcatgaaa ccgctaatct tcgccctcgc
    13681 cgctcttggc ctcgcccttc tgcactcgca atgctcgccg gacaaggccg gcgcggcgga
    13741 tgctccgggc gggaaaaaac ttgtcgtcat gatcgcgggc agcccttccc acggccccgg
    13801 acagcacgag cacaacgcgg ggattttgct gctgaaaaaa tgcctggagc agggcgcggg
    13861 aacgcaggtg gagatcaagc accaccttaa tggcgggtgg cccgccgccg atgaactggc
    13921 gaaggccgac accgtggtca tttacgcgga cggcggcggg cgccaccccg ccctgcagga
    13981 ggaccgcctg gcgcagctcg acaaggaaat gaaacgcggc tgcgggtttg ccaccatcca
    14041 ctacgccgtg gagccgacca tcgagaaggg gcagaaggaa ttcctcgact ggatgggcgg
    14101 ctgctttgaa atcaactgga gcgtgaaccc gcactggcag gcggagttca aggaatttcc
    14161 cgcgcatccg gtcgccagcg gtgtgaaacc cttcggcacg ctcgacgagt ggtatttctt
    14221 catgcgcttc cgcgagggca tgaagggcgt gacgcccatc ctaagcagcg cggcgcccga
    14281 gtccaccatg agccgcccgg acggccacca cagcggcaac cctcatgtgc gcgcctccgt
    14341 ggcgcgcgga gacaagcagc atgtggcatg ggcctctgaa aacgcgggcg gcgggcgcgg
    14401 attcggcttc accggcggcc attttcatca gggctgggcc aatgacgacc agcgcaagct
    14461 cgtgcttaac gccatcctct ggaccgccaa gggcgaggtg cccgccgccg gcgtcgcgtc
    14521 cacggtcact gacgaggaca tgaaagccaa ccttgacctc aagcccggcc agggcctgcc
    14581 cgaaaaggcg aagaaagcca agtgacgcgg cgcgcccgca gcatcacgac ttcctttccg
    14641 ccacggtgag atggcagccc gagagaatgc aggccgcgcc ggccgcctgc gtgagggtga
    14701 aggtctcgtc aaaaaacagc acccccgcca ccgtcagcac cagcggcagc agaatctgga
    14761 aggcgccgcc cgtgctcaca ggcagatagc gaaaaccctc cgtcatcgcc aactgcccca
    14821 atgccgcaag cagcgcggcg gcggtcagca gcagaatgtc caaaccgccc ggcagcggca
    14881 gcgaaaaaag aaacggcagc gccaacgcgc cggcatagat gcactgggag gcgaaaatgg
    14941 tgccgctcgt ctccgtcaga gtgagctggc ggatgaccac caccacaaac gccgccgcaa
    15001 aggcacccgc cagagctatg gcatcatacc cgccgacccg ggaaaggtcc ccgccttgca
    15061 gcccggtaag cagcgccacg cccgccaccg ccaggacgat gcccgccagc ttccgcaccg
    15121 cgagcttttc ccccagcatg aaagctgcaa acagcgccgc ccacaccgcc caggtgttgc
    15181 cgatgagcgt cgccttgccc gcgcccagca caggcagtgt cacataatag gccgccgtgc
    15241 ccagcgcgcc gagcacgccg cgtgtcacga gcagtcgccc gcgcagtgcg cggcgaaagc
    15301 tgacacgccc gccgggagcg aagaccgccc acaccaccgc catgcccgca gccgcccgga
    15361 aaaacagcgc ccaccacgcg ctcaccccgc gctccgcgcc cagatgccgc accagcagcg
    15421 ccatgcccgt gaatcccgcc agcgagagca gcatccacgc cgatccacgg cggaggttaa
    15481 ccatggggac ggcagtgtcg tgggttgtgt tcaagcagga tgaatgacgc gccccgcgcc
    15541 gcgctcaagt cccggcttgt gaaagcaagc gccatcgcta gcctcggccc atgcgcgccg
    15601 cactcctgat cttccttgtc tgcacaaccc tgcacgccca gcagccgccg tatgatgcct
    15661 tccccgctgc cgacccgccc tactaccgcg tgcgttacga gccctcccgg acagaaggcg
    15721 gcctcatctt tggagtgaat tacaccctgt ggattccccc cggcgtgaag accctgcgcg
    15781 gcgtcatcgt ccaccagcac ggctgcggag agggatcttg caagtccggt ctgaccggcg
    15841 cctacgacct gcattggcag gccttggcga aaaaacacga ctgcgctctc atggcccccg
    15901 cctacgagca gcctgacaaa gccgactgcc agatgtggtg cgacccgcgc tacggctcct
    15961 cggcggcttt ccaaaaggct ctctccgacc tgggcgcgaa gtccggccat cccgaactgg
    16021 ccgaagtgcc ctgggcgctc tgggggcaca gcggcggcgg gcactgggca ggcggcatgg
    16081 ccctgctgca tccggagcgc gttgcagcag cgtggctgcg ctccggcgtg cccctcctca
    16141 ccgcgaatcc agaccgcgcc accatcaagc cgcacaccct ggatgatgcc gtgccagccg
    16201 tgcccatgat gtgcaacccc ggcaccaagg agggcgtgac cgtcaaggaa gggcgctttg
    16261 ccggcgtgtg ggccgccaac gaaacctttt tcaaaaccct gcgcgccaaa ggcggtctcg
    16321 tcggtgtggc ggttgatccg ctcaccagcc acgaatgcgg caaccagcgc tatttggcga
    16381 ttccctggct ggacacctgc ctcagcgcgc gcctgccgga caaagcaggc gaacctctgc
    16441 gcaaaatgaa tccagacaaa gcctggctgg cgccattgct gggcaccgga gcggcccccg
    16501 ctgaaaaatt caccggcgac actcaaagcg ccgtctggct gcccgatggc ggcacggcaa
    16561 aactttggcg gcattacatc acggacaccg tcgtgccgga caccacgccg ccgcccgctc
    16621 cggtgaacct gcaagtcaat ggcaggacgc tcacctggga gtgcgaagcc gacctcgata
    16681 gcggcctggc cggattcctc attgagcgcg acggcgtcat catcaccccg gcccccattc
    16741 aggcaaagaa cccctttggc cgccccgtct tccagggact gcaatacagc gacacgcccg
    16801 ccatgccgct ggtcgagatg tcctacaccg acaaggatgc cacgccaggt ggcagccacc
    16861 gctaccgggt catcgctttg aacacggcgg gtctgagatc ggagtgacgg cattcctgcc
    16921 gccgccagca tcccattcat caccgcgatg ccgcgcgatc ccggccaagt gccgctccag
    16981 cgcctcctgc catgccggga tgggcgcgcc aagaacttcc gaaaggcgcg cgttgctcat
    17041 gaccgtgaaa ggcgggcgcg ccgccttgaa gtggctgaac tcgcgcaaac gcctgcctat
    17101 ggtgtggcac gggcgctcga tcaacccggc ggcatgagcc agatcgagcg tcacctgcgc
    17161 gtagtcccgc catgtcgcca cgccagtgtg gcagaggtgc agcacgccgg actgcgggcg
    17221 caggtcggtc acaaagcgct ccagccacgc ggtcaagtcg gcgatgcccg tgggcgcgct
    17281 ccatttgtcc tcgatgaaat ccagcggctg cccggatcgc gcgcgctcca gcaccgtggc
    17341 gggcacatcc ccgccgcgcg ggccgaaaag ccaggacacc cgcgccacca gcgcctcggg
    17401 gcatgcggcg agcacggcct tttcacccgc gagcttcgtg cgtccgtaaa cgttcaccgg
    17461 acgcgcgggc gcgtcctcat ccagcagcgc gccgccgcct ccgtcgaaaa catagtcagt
    17521 gctcaaatga atgagccgcg cgcggcgccg ggcgcattca gcggcgagca cagcgggagc
    17581 ggcggcgttc agcgcggccg cgtcatccgg gcgctcctcg caggtgtccg ggctggtcag
    17641 gccggccgtg ttcaccagtg tgtcaaactc cagcgcgcgc agctcccgcg ccagcgccgc
    17701 gggctgcgac gcatccagca aacgcctgcc cggcgccatc acctcatgac gcccctgcca
    17761 ttctcgcgcc agcaccccgc ctaggcggcc cgtgccgccg aggatgagaa tgcgccgcgc
    17821 tgttgacatg ccgccatcca tgacgccccg cgcgctgtca tgcaagcccg gtgcagcctt
    17881 catcgcccgc gcccacccct ccggcatgcg cgggaaacat gggtggcagg cgcgcgttca
    17941 tgtgcttgac tcggcggctt catgttccgc gccgcttcca ttcaaccgcg tgccagtcat
    18001 ccgtcactca ccgccggagt gtggcggtgc ctgccatggg cggcccttgc gcttgccatg
    18061 ccgctggcat ccgccaggga gcggacatat ttttgcctgg tccatgatcc gcgttcgccc
    18121 gtggagtatt atgcgaatgc caccggcggc ggacgcggcc tgttcggcgg cacggatgag
    18181 gtggcggtca cacgcgccgg agaggcggcc tggcgcgtca gtgtccgcgc caccaaggga
    18241 gagtgggctt acggcggctt catcaacgcc gtcacgcgcg ccgtggagga ccaggtaatg
    18301 gtgaacccaa acgcgctgtg gcaccccatg atcctcaagg aattccagcc gcgcttgaaa
    18361 tccatccggg ttgaactggc aggggcgcgc tcacccgccg ggcgcgcgga cctgcatctt
    18421 cggctggaac tcaaaggctg ggacaaaaac ggtgcgcagg tgcctgcgca ggcctttgct
    18481 gcggatcgcg ccactttgct aaacggtccg catcccaaaa aattgagctt tcctgtggat
    18541 gccatgaccg gcccggccgg cgcaatgatc gtgatgctcg ataatgcggc ggccggcgac
    18601 gaggccgaga ttggcgccat tgagtgggag gtggcgcttg gagagacacc gctgccgcgc
    18661 cgcgccgctc tgctgggcct cggagcactg ctggcgaact gggactccag cagcggcatg
    18721 gctcaggacc gcggcagttt cccggcgggg atctatgaaa acctgactgc cacagccaag
    18781 ctggcgaaaa tcatcgcact cgcagcgcgg gaaggcatcg tggacgaaac ggcgggtaaa
    18841 gccgccatcg aaaaaatcgc cgccacgctg cttgatgtgg tgccgcgagg tccgcagccc
    18901 tccaaccgcc tctggccgca tttcacccgc aacggcggca cggcacgcca cgaaggaagc
    18961 gaatggtcca gcggagacac cgcctacgca gtggccgacc tggctttggc tcttgcgctg
    19021 acaggcgacg cccagaagcg gcttccgcaa ttgatggaga tgctgcgggg catcaactgg
    19081 cgcatgctgc atgacagggg cttttaccgt catggctacg accgcgatgg aaaaatcatc
    19141 ccccatcgct ggcagggttt cggcgtcgaa accctcggcg taaacctggc cgcgcttgcc
    19201 ggcggcgggc ctccggcgga aatgctgccc ccacccacag acgacggttc cggcttcaac
    19261 atgcacgcgg cctatcccct gccctttgcc gccgtggact gtcatggcaa cgactggcgc
    19321 cgactgaggc tggaggagat ccgcgcgcaa acaggctggc accgccaaac tgcgcgcgcc
    19381 aacacccgtc ttgctgacgc gcacctcttc ggcctgagcg cggcggagac acccgacggg
    19441 cagaactaca tggcctacgg catcggcggc aggctcgatg gcgcgcgtga cggcggcggg
    19501 cgcatcgtga ctccgcatta ctgcggcatg gtggcggcac tggctccgga ggatgcggcg
    19561 cgcatggtgg agagcctgga agcgcgcggt tggctcacgc cgctgaactc cgccgactcc
    19621 gtggagattg accctgtgac cgggcgtgaa accgtgaact ggatgaaggg cagttggaat
    19681 ctcgcgctat ttaccgaggg ttggctcttt gccaatccag acatcctgag tgcggcaaat
    19741 gaggcgttgc gctcaatccc cgagttcgcc agggcatggg acctgtgttt cccgccgcgc
    19801 tgaccaacaa ccttgcttcc ggtcttcgaa atcgccgcct ccacctcctc atgcgtggca
    19861 ttcgtggaaa gctgtgcttg agtctgcggc ttcatgaccc acgccgttct cagtctcctg
    19921 cgggaaagcc gccatcccat gcgcatcaac ctcatcggcg tggccggctc gggcatgagc
    19981 gggctggcct ccctgctgct ggcgctgggg cacaaggtga gcgggtcgga caaggtgacg
    20041 acgctggaga cggcgcgcct gcaaaaactg gggctgcaat ttttctcccc gcactcggag
    20101 caggaggtgg cggagtgtga catggtcatc tactccagcg ccatcaagcc ggggaatgtg
    20161 gcctatgagg cggcctacaa gcagggcatc ccgctggtgc gccgcgcgga ggcgctggcg
    20221 gcggtgatgg caaataaaaa aggcatcatc gtcggcggca cgcatggcaa gacgaccacc
    20281 tcggcgctga ccgcgcatgt gctgcgccag ggcgggctga agccgagtca ttatgtgggc
    20341 gcggagatcc ccatcctggg ctcgaacgcg cactgggaca cgggcgggga gctttttgtg
    20401 gcggaggggg atgagagtga tggcacgctg gtccacttcc acccgcagca cgccatcatc
    20461 ctgaacatcg aggcggagca tctggacttc tatgacggca ttgaggccat caaggcggtg
    20521 ttccgccaac tgctggcgca gacgccgggc tggaaggtgt attgcgcgga ggaccccgtg
    20581 gcacgcgagg tgtgcggcgc ggcggaaaac gcggtgggtt atggctggag ccgggagatg
    20641 gatttctcgg cggagatcgt ggaaatgaag ccggaccaca cggacttcat cgtctggcaa
    20701 aaggacgagc ggctcggcga ggccacgctg ggcattcccg gcaggcacaa tgtgtcgaac
    20761 gcgctggcgg ccatcgcgct ggcgacgcgg ctgggggtgc cctttgagaa catccgccac
    20821 gcattgaaaa gcttccgcgg cgcgcggcgg cgcttcgaga tccggcatgg cggcgcgcgc
    20881 ttcacggtgg tggatgatta cgggcaccac ccgagcgaga tcgccgccac gctggccacg
    20941 gcgcgcggac tgaagccggg gcgcatcgtg tgcctcttcc agccgcaccg cttcagccgc
    21001 acgcagcttt tgaagaagga gttcggcgcc agctttgacg atgtggacga gctttttgtc
    21061 accgatgtct atgcggcgag tgagaagccg ctgccaggag tcagcggcgg caccattgtc
    21121 gaggcggtgg aggcgcacaa ggcggccgcg ggcggtgtga agacgcccgt ctgccacagc
    21181 acgccggcct tgctccaggc tcgggacaag gcgggcaacg cgctgcgccc cggagacctg
    21241 ctcatcactc tcggcgcggg caatgtgcat gaggtgggcc gctgcatcgc gcgcgacctg
    21301 cctgtgctgg agaagctgtg ggagctgctg gaggcgcatg gcggcggcgc ggcgcggctg
    21361 tatgagccga tgaaccggca cacgacctac ctcatcggcg ggcaggcgca gttctgggtg
    21421 gagccgcgca cgatcggggg cttcgcggaa gtggtgcgtt acctgcgctc ggccagcgtg
    21481 cccatccgcg tcatcgggcg cgggtcgaac ctgctggtca aagacggcgg cattcgcggc
    21541 gcggtgatcc atccggcgaa gggggagttc gaggaagtgc gcgtcgaggg ggagacgctc
    21601 atcgccggtg cgggggcgcg cttgaagaag atcgccagca cggcgcggaa cgcggggctg
    21661 ggcggctttg agtggatgga gggcgtgccg gggaatctgg gcggcgccat ccgcatgaac
    21721 gcgggcgcca tgggcacgga gacctttgat caaatcgtca gcgtgcgctt catagacgtg
    21781 gatggcgcgc tgcgggagaa gcccctggcg gagatcacgc atcactaccg cagcgtgccg
    21841 gagttcgagg agcgctacat cgtctccgcc gtgctgaaag gagcgcctgc tgcgcgtgag
    21901 gagattgatg agaggctggc cgcctcccac cacaagcgcc gcaccaccca gccggtgggc
    21961 gccagcgcgg gctgtgtctt taaaaacccc gaactctgcg gcgcaggcaa gctggtggat
    22021 gacctcggac tcaaaggccg cggcgtgggc agggcggtgg tgtcacccgt gcatgggaac
    22081 ttcatcgtga acaccggcgg cgccaccgcg cgcgaggtgc tcgacctcgt ggccgagatc
    22141 caggagaccg cccagcgcga gcgcggcgtg agcctggagc tggaggtgaa ggtcatcggc
    22201 gaggaccacc cgctgccgct ttgacgaacc gaaccgccgg gatgcatcaa gcatggtgaa
    22261 ccacgaattg acacgaacag acacgaatgg gatgccacgc ggcagcttca tcctgctcca
    22321 gtcattcgcg tccatttgtg tccattcgtg gtttttaatc cgcccatgaa atccccctgg
    22381 ctcatccccg ccctgctcat tcccctggtc atcctgggct ggctcaggct gcgctttgag
    22441 acggacatcc tggccacgct gccggcggat gtgccggagg tgcgcgcggt gaagctgctg
    22501 cgcgcggggt tcaccggcgg cacgaacctg gtcatcggcc tggaggcgga ggatgacatc
    22561 ctcgccgaag aagccgccaa ctcgctcggc agacgcctcg aaaaagaatc cgcgctggcc
    22621 tccgaggtgc gctgggcgcg gcccgtggag gagcaggcgg agacgggctc ggcgctgctg
    22681 gcctggatgc tgcaaaacgc cgagcctgcc gagtggggaa agctgcgtgc tcgcctcgaa
    22741 ggcgatggcg cgaaggcgca ggtggcgaag tctttccaca ccgtcggcca ttccctggac
    22801 gcggagaagg tgcagcgcgc ctcgtatgat ccgctggggc tcatggacgc gctttcgctc
    22861 gatgacctcc agtccatggg cgactcctcc ttcggcctgg cctcggagga cgggcgcttc
    22921 cggctgctgc tggtgacgcc gatggccgag gtgggcaatt acaaggacgc ggacaagtgg
    22981 ctgcaacgtg tccgggagat cgtcgccgca tggaaggggg aggagggctt tgaggaatgc
    23041 gccgtgcgcc tcacgggcga gccggcgttt caatccgagg tgggcatggg catcgaggag
    23101 gacatgtccg gcaccatggg tctcacggcg gggctcatcg ggctgctgtt ctggctgatg
    23161 ttccgccgct tgaagctgct gctgtggatc cagctcctgc tggcgctgag catggtcacg
    23221 gccatcggca tcggcgggct gctggtgggc cggctttcca tgatgagcct gggctttgcc
    23281 gccatcgtca tgggcatcat cgtggactac gcggtgctca tcctccagga ggcgcggcag
    23341 catccgcagc tgcccgccgg gccgctgcgc cggttggccg cgc
//
LOCUS       DAOMYJ010000065        23267 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Prosthecobacter sp. isolate AMR_MDS_5629
            NODE_792_length_23267_cov_22.363605, whole genome shotgun sequence.
ACCESSION   DAOMYJ010000065 DAOMYJ010000000
VERSION     DAOMYJ010000065.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867850
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prosthecobacter sp. (wastewater metagenome)
  ORGANISM  Prosthecobacter sp.
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Verrucomicrobiaceae; Prosthecobacter.
REFERENCE   1  (bases 1 to 23267)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 23267)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.30
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:34:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,742
            CDSs (total)                      :: 4,684
            Genes (coding)                    :: 4,676
            CDSs (with protein)               :: 4,676
            Genes (RNA)                       :: 58
            tRNAs                             :: 55
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23267
                     /organism="Prosthecobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_792_length_23267_cov_22.363605"
                     /isolate="AMR_MDS_5629"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1965333"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            224..961
                     /locus_tag="PLP58_08865"
     CDS             224..961
                     /locus_tag="PLP58_08865"
                     /inference="COORDINATES: protein motif:HMM:TIGR02436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four helix bundle protein"
                     /protein_id="HRJ08665.1"
                     /translation="MAERLHHEDLRVYQKAVAFVAQASDILEPVSSKHAVKDQLLRAA
                     ESMPLNIAVSNASQSQASQKQALETAFSSAAECAACLDVLQRKQLIAGDLCKTGKLEL
                     QEVFHMLMGLWKSKEDRLCEDAPEPLSTGFSHEKLECYGRGLHLIGWVTDFCHQTQVP
                     QRSQEVLDRSVTSLVLNLAEGNARWALKDRAHFFDLSVMAGLRFAATQDILVARSLAG
                     IETVSEAKREVAIAVRQILGIKRKEML"
     gene            1066..3474
                     /locus_tag="PLP58_08870"
     CDS             1066..3474
                     /locus_tag="PLP58_08870"
                     /inference="COORDINATES: protein motif:HMM:NF019259.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1592 domain-containing protein"
                     /protein_id="HRJ08666.1"
                     /translation="MATDPRVFLNDYCISCHGPDKQKAERRFDQLALPATTAEHVIDL
                     QDIIDQLNLGEMPPKKSKQPTDAERSAMAAALTEVVAQARSALRSTGGQTVLRRLNKR
                     EYLNTVGDLFALDMRLFDPTTKFPRDQMAEHMDNLGDVLQTSGYLLAQYLDAADQVVE
                     RAFSVTGKPEEKTWRFTDNFRPQQEHTFPHGAVYKNRYLCLYEVPDTENHEGGYGYIH
                     DFKQGVPVDGLYEIRVKAQAMHREHPYDPAIFQRDTAQPFRLGIVPGDVKAGPLHHPQ
                     PIEPQLAEVTLKDGEAEWHTLKVPLLAGQTPRFIFPNGMANSRRAFGTLARKYQDQWP
                     EKERKDLGIFQARRVVLQYGKMPHIRIHEVEIRGPIVQEWPPVSQRSVLGEAGFEPER
                     RREILQSFASRAYRRPATADEVDRLMRVVDTRRQAGSTPFEAMKDGLKAALCSPAFLY
                     LADPAAKEEKLSAHAFATRLSYFLWSTMPDAELRALADSGELLRDEVKLEQTRRLLAS
                     PRAEAFIEGFLDAWLNLRSLGDMPPDRDAFARFYADGLKPAMKQETRLFMRHLLDQNE
                     NLYRFLDADFTFVNQPLADLYGLGRISPPEAAQEFRKVSLTDRRRGGLLGQASVLTVS
                     ANGIETSPVVRGIWLLENILGTPPPPPPDNVPPIDPDIRGAKSMREILTKHRDNTGCY
                     ECHAKIDPPGFALENYDPIGQWRTHYPAGKKQGPKIDASGEMPDGARFEDITGFKALL
                     LKKEAQFRKTLAERLLAYACGRRMEGLDRPQVDAVLAVVEKEGNGLRTLVEKVVLSEV
                     FARR"
     gene            complement(3505..3987)
                     /locus_tag="PLP58_08875"
     CDS             complement(3505..3987)
                     /locus_tag="PLP58_08875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ08667.1"
                     /translation="MGYGATLGTWHLGGKAPLEVTCTWNVPQGIEVDEHSVAVLRYTC
                     GLSKTETRWGTFTDPWTHQPQPMCGFVLRGTQGTISCYDYARHIRVQTRARPEGFEVP
                     VDVIPSPNRNPIEHLIHHLETGAPLIGPLTLEISRIGQQIVDTAFLSAQQKRALPLVG
                     "
     gene            4242..4694
                     /locus_tag="PLP58_08880"
     CDS             4242..4694
                     /locus_tag="PLP58_08880"
                     /inference="COORDINATES: protein motif:HMM:NF012330.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-protein phosphatase"
                     /protein_id="HRJ08668.1"
                     /translation="MTSSNKPSIEIPGHLLASSRPGYDSGRNHPVSRDAVESWINVAR
                     EAGAASILCLLDERHLVLYEEALPGGLLAHYEQSGFDVGHLPIRDHEWPPVTEDELRQ
                     AQAWFEKLPKPVLVHCSAGQDRTGAVVEHLCALHENQAKPSTDTLASP"
     gene            4691..4933
                     /locus_tag="PLP58_08885"
     CDS             4691..4933
                     /locus_tag="PLP58_08885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ08669.1"
                     /translation="MKNNFFRERWLKRLGLPDADWQEPMQRHLESLPFLDASEKKAAR
                     AMILWLLEKLPARLLRDPTESTQRLAEAFGCACLAF"
     gene            4976..5743
                     /locus_tag="PLP58_08890"
     CDS             4976..5743
                     /locus_tag="PLP58_08890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ08670.1"
                     /translation="MHLQAQLKLPAAKRQPGTQLLVSLLLDASTDGACGLNRLELADA
                     EVVRASERLIGEGRFEDYIKLPEKFAEYDTRLREHRGFKHDWECLCQQYPAQAAATGI
                     LHRRLIPERNWERGPGAEFTNEDQCFQAAFDLFCWKYYLWGVKDGAPLLLKPSVVFTP
                     FGTQIFIPGYMSFDARRDLDFRRINALHKARGVTRQGPAFSAGRIETVEKKKRVKAAK
                     KQAIQKGLKGEARYDYISQKSGIRTQGDHRSLRRLAE"
     gene            5833..6006
                     /locus_tag="PLP58_08895"
     CDS             5833..6006
                     /locus_tag="PLP58_08895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ08671.1"
                     /translation="MSQRLFEISYILRNSSNSATQRAYVNASDTVMARRIFEQQNPAC
                     KIVSSPKDVTDQR"
     gene            6047..6409
                     /locus_tag="PLP58_08900"
     CDS             6047..6409
                     /locus_tag="PLP58_08900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ08672.1"
                     /translation="MTPFESLLHKLQPRHRLIGAVGLLLLLFLAWSDQAPAQQGKWLD
                     YGSGMLNLAVVKSIGPSSRNHLTFGSPPGYLEFDGMRLVFQSPAEQDSALREIRSFLV
                     CGDSYHRLQAELKGDTAP"
     gene            6437..7246
                     /locus_tag="PLP58_08905"
     CDS             6437..7246
                     /locus_tag="PLP58_08905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ08673.1"
                     /translation="MKYPDLVRSVQKHNRNWVCESFPEDKGVWSEDESSIKIHGAVHI
                     ADCSNSLCGFTKTADDVTQLVEKWLTLTDRNKQECRVAVASVVDKGTRDSMDDLPAVL
                     AVGINYGQGDWYADHAVPLEDNTQLRSQASKVLQELKQRSCCLDEMASFHLVAANFFP
                     WITQWTWGDLNSIEESILLRGSGHPDSVGHVVGLIQKIRPQVVMFHGANNAAPGLGWH
                     VMDRLCGPSWNRADCKFVFSDNLAPPNRAISNAVMTPCPVGRHNLPPGFEA"
     gene            7243..7713
                     /locus_tag="PLP58_08910"
     CDS             7243..7713
                     /locus_tag="PLP58_08910"
                     /inference="COORDINATES: protein motif:HMM:NF019416.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1643 domain-containing protein"
                     /protein_id="HRJ08674.1"
                     /translation="MKNPPLYHAVFSSCRRYRYEWHYRWGEGPVCMFIGLNPSTADDF
                     QMDPTVRRCVDYVQRWGFGALCMTNLFAWRDTDPEAMKQQPQPIGPKNDATLKRLAAK
                     ADLVIAAWGCHGSHLQRGDAVRQMLPNIHALKVNQDCSPAHPLYLSKKLQPISF"
     gene            7772..8845
                     /locus_tag="PLP58_08915"
     CDS             7772..8845
                     /locus_tag="PLP58_08915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ08675.1"
                     /translation="MNDLDALLAALADMPEPHFASAPERLRNLREYLTALQQGSFSGD
                     FLVGDACLSQSASLTGIPFTSEFLLRADAHPFLDSLRPRLWVEGRQFEQASYELWMAL
                     GDGQAVPGLWQLFHLKAGDDAEECEASLFRHLLRVLKPRRIFAWGKDVADFLGRQFPE
                     LEVRPLLARRSSWHEEALRLELEWNGVIKASQEESQVSRGSELGQLYLKVLPRHAIAD
                     TATASREERQNCGVAAAGVLDDQCRFTFFRHGQTAALARMLQQARQVVGYDCLIFDFE
                     LIRSETKFKRPPALDLRQEIEDATGKRLSFENVARTVLGILPGSTDELMRVLVAQADW
                     ENVSLHLETYLTLLTKLHHQLSE"
     gene            complement(8895..9182)
                     /locus_tag="PLP58_08920"
     CDS             complement(8895..9182)
                     /locus_tag="PLP58_08920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HRJ08676.1"
                     /translation="MPSVFIAPDDTVFDKNVSNIEEVLARKGPVIAVTTEGRTELERL
                     TEDVIYVPDCPSYLSPLLTVIPLQLFSYYTAVARGCDVDKPRNLAKSVTVE"
     gene            9166..9504
                     /locus_tag="PLP58_08925"
     CDS             9166..9504
                     /locus_tag="PLP58_08925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ08677.1"
                     /translation="MNTLGNGDGTPVYIEYLAGTFAFLSSETLSLTITGWHTDGSAVL
                     SAFIVVNKTYRVEFSENMKDWTRTPVRADSPTAEVLTTFTATDTREISLYSAASIGKP
                     HLFYRMVLVR"
     gene            9511..9777
                     /locus_tag="PLP58_08930"
     CDS             9511..9777
                     /locus_tag="PLP58_08930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ08678.1"
                     /translation="MEDLTNHPSRSAAAAGALAEWRTESFALKAGWNAIYPHVDSSHA
                     TLDTLLAAYPDVHEVWRWQPERVDPREPGDVNAPPARFGGAGGA"
     gene            complement(9830..10900)
                     /locus_tag="PLP58_08935"
     CDS             complement(9830..10900)
                     /locus_tag="PLP58_08935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009961078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HRJ08679.1"
                     /translation="MKRWRIAGINFDHFHMGDLLRQTHEHPAAEIVGISDEFPERMEN
                     AARTFGLGAERVFTDYSACLEATKPDIVILCPAASRHGEWVEKVAPYGAHILMEKPFA
                     GSLAEADAMMAAMPPTGKVLSVNWPLVWDAGQQTAHRLIQEGVIGEVVEFHHHGGNRG
                     PLCHGADKQDKEPTAEEKAASWFYSAAQGGGSLLDYMGYGATLGTWHLGGKAPLEVTC
                     TWNVPQGIEVDEHSVAVLRYTCGLSKTETRWGTFTDPWTHQPQPMCGFVLRGTQGTIS
                     CYDYARHIRVQTRARPEGFEVPVDVIPSPNRNPIEHLIHHLETGAPLIGPLTLEISRI
                     GQQIVDTAFQSAKLKRTLPLVG"
     gene            11623..12675
                     /locus_tag="PLP58_08940"
     CDS             11623..12675
                     /locus_tag="PLP58_08940"
                     /inference="COORDINATES: protein motif:HMM:NF012652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase, T2SS/T4P/T4SS family"
                     /protein_id="HRJ08680.1"
                     /translation="MALTLDDILQSADTHQATDVFLMEGETPRMKIHEQLMTLGESVM
                     QGGDMAALWRACGGDPESDTDRDSSLVSPSRTRYRVNMHKALGRLGVVMRRIKSELPV
                     LSELGVPDWLLTRWAMRARGLILITGPTGMGKSTTMAGLLQWLNENTARHIVTIEDPV
                     EFIYGNQGCMFTQREVGRDTATYARGVRGAMRQSPDVIMVGEIRDLETALATLQASET
                     GHLVLASIHSDSVVDAVDRVAHLFPPEQAGLGLYLLSHQLIGALCQKLIPRVDGGVHL
                     ICEHLENMGAVKPWIAAQEMGKVREYMQRGNDPNCRTFMHDLVQAYEAGVITEEEAVI
                     TSGNEAEFRRAARGIS"
     gene            12686..13507
                     /gene="thiD"
                     /locus_tag="PLP58_08945"
     CDS             12686..13507
                     /gene="thiD"
                     /locus_tag="PLP58_08945"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008902 - hydroxymethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0008972 - phosphomethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HRJ08681.1"
                     /translation="MKAPPPVLTIAGSDSSCGAGAQADLKAITALGGYALNALTSVVS
                     ETPGRVSKVCLLEPGIIADQMRVLFEGFPIAAAKTGMLGGKAQVEAVVENWRALAPPG
                     LPLVADPVMVATGGGKLLEDDAIQAVRELLLPLAALVTPNMDEARVLWGRDVRTRADM
                     ERCARELADVLQTSVLVKGGHLEGGAAADVLCADGTITWHETERVPGVRTHGTGCTYS
                     ASIAACLALGLPLADAVARAKAYVAGAIGGFLSWQGARGEVHALHHFAGRLQDAS"
     gene            complement(13492..14562)
                     /locus_tag="PLP58_08950"
     CDS             complement(13492..14562)
                     /locus_tag="PLP58_08950"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014673.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HRJ08682.1"
                     /translation="MTILDDILIAEASAAPDGRLTFARVMELALYHPEHGYYGPAPRR
                     IGRAGDFFTAVSAGQLYGRLLAGLAERTWNEMGKPGEFCIIEQGAHDGQLAHDMLSAC
                     GLPLRIVEPNPRYQAAQHARLADFQDRVSWTDSLDELPPCPALFVCNELPDAMPEHLL
                     RFESGEWRELHVAWEGGRAIFKSGPLSSGRLRGEAARLPVNLPEGHMMEIGLAALDWM
                     EALARSAIHGRIFIADYGLDEEEFFSPERAGGTLRRYHSHQSDDRVLEDLGRCDLTAH
                     VNFTRLAETAASRGLSTLAYEHQGRFLGRLGMDWLRSLDGAPPDAATRALLRQYHTLT
                     HPAFLGRTFRVLLLEKPHQLAS"
     gene            14631..17171
                     /locus_tag="PLP58_08955"
     CDS             14631..17171
                     /locus_tag="PLP58_08955"
                     /inference="COORDINATES: protein motif:HMM:NF013100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HRJ08683.1"
                     /translation="MFGDDDLQDPSKKNWRGEPALKPRFYRRAWFSALMALILLGGVS
                     LLGVWAIVVAPLKEKAEEYDLDEVRKLESASIIYDRNGEELSRIFVLNRTPVSIQEVP
                     KHLINALIAQEDSRFFRHNGVDYIGLGRAVIENLKAGRTTQGASTITQQLARQTFGLM
                     EKSYKRKLMEAFVAQRIEKKYSKTEILELYLNRIFFGKNFYGIQAGARGYFGKDTKDL
                     TIEEAATLAALIKSPNNIQPLDHPARAVKERNYVFDRMVAEGLLDRVEAEKAKIKPMV
                     TAPQGNDPRLSYAFDEIRAQVMSIVGEERAAVGGFLIYTSIDKDLQNAAEAALKKRLA
                     EVEARPGYAHQTYDQFKSILRDWRAGLSKLAADAEAPPRPIPEYLQGAALVIDNRDGA
                     VLALVGGRDFVDSQFNRVSQGSGRPTGTSFIPFVYAAGFSKPDFFPMSQLADEPLGND
                     RVMIGGFTGILGEYGMEVADPKWSRKPISAREALVASHNSATVRLGERVGLERGLVTP
                     QFRDFVKKTGINAPLRDGPATFLGASEARLDEMVLAYSSFPTLGRRPAKLHIIQRVTD
                     AEEKTVYQVPEESLQTVRAMDEIAAWQTHTCLDEALHRGTGSPAVSEYGLGDFPAAGK
                     TGTHHEYKDLWFLGYTSAVTCGVWTGFDRQRTIHDMAFSNRITLPIWTDIINASIKTH
                     APQDFSPPESAERIEICRASGLRATDFCYEKVRGSDGVERSVRNTYIEFVRPGTTYNA
                     TCAVHTGEGLPADLAAFHQTLTQSSESPLLADAGRFVNVEPVHMRDPLIIGADPYNSI
                     MPVLRARPVNDDGSPILRALPVGPDEGGAPEEAPVIRLKPPPPMKIEL"
     gene            complement(17252..17476)
                     /locus_tag="PLP58_08960"
     CDS             complement(17252..17476)
                     /locus_tag="PLP58_08960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ08684.1"
                     /translation="MNALNTPAIEMDLGWNSMPAFGSRTQLSSTDAVAASEFVAGHSG
                     DAPAKPGLTFWSQFATPTSILPAAAGACMG"
     gene            complement(17602..18279)
                     /locus_tag="PLP58_08965"
     CDS             complement(17602..18279)
                     /locus_tag="PLP58_08965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ08685.1"
                     /translation="MKTNSKILIALATLVFAAASKEAAAQNGYYAAAHYAAQQAAYAQ
                     QQAAYQAYVQNQQAWAAYYAQQQAMAAQRAPAPRATPVAAPRATGSLIRFDGQYASCS
                     ASLPTQVQYLINAANHLQSSPYVRGAGHGRVEDRAYDCSSSTSYALIKAGLLKTPLTS
                     AGFANYGHPGEGRFITIWVKPGEHVFMTICGLRLDTSGGQVGEGPRWRTKGRSYAGFT
                     PRHPAGL"
     gene            18854..20146
                     /locus_tag="PLP58_08970"
     CDS             18854..20146
                     /locus_tag="PLP58_08970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009965611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1501 domain-containing protein"
                     /protein_id="HRJ08686.1"
                     /translation="MNPTSTPFGRRQFITGAAQTFLGVTAMSTLAGKALAAGKDASTA
                     RQAATARNVIYLYMNGGMSHLDTWDPKPASSGVMGLTKTIKTRADGIQISENLPLLAR
                     QTDKLAIIRSMSSTQGAHEQGNYFMHTSYTLRSSIRHPSMGAWLQKFQDRGNPTLPGS
                     VMIGNDSRHPGAGFFESRFSPLMINNPESGLANVRVNDWFTEERFSSRLEVAKKLDRK
                     FAETYDVKNVRAYSDMYDDAVKMMKSEELKAFNLADESDALREKYGRDRFGQGCLLAR
                     RLVEHGVRFVEVSFGSWDTHNANFTRVPELCEELDNAMSALLADLESRGLLEETLIVL
                     ATEFGRTPEINANDGRDHHPAAFSCVLAGGGVRGGQVYGSTDEKGARITGNPVNVPDI
                     NATIGYALGLPLDQVLYSPSKRPFTVADKGKPLTTLFG"
     gene            20206..23181
                     /gene="uvrA"
                     /locus_tag="PLP58_08975"
     CDS             20206..23181
                     /gene="uvrA"
                     /locus_tag="PLP58_08975"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009961838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HRJ08687.1"
                     /translation="MPGAQARGLTPVASPAFNGGMAQDLIRVRGARQHNLKNIDVDIP
                     RHRLVVLTGVSGSGKSSLAFDTLYAEGQRRYVQSLSAYARQFLDQLEKPDVDFIEGLS
                     PAVAIEQARGAPNPRSTIATVTEIYDYLRVLYAVAGLPYDPQTGAPLSRTTPAEMGAR
                     ILELGEGARVVVLAPQPPADADGLRALFEKLRRQGFVRVRLDGVIHELDEPPELEQGR
                     TCAVEIVVDRLVIREGAQARLMESIEAALRWNENEARFLVQARGGGEETLSFTTAYVN
                     PETGYTLGRLTPQHFSFNTHLGACPRCEGVGAVMAPDPGLIVPDARLSLKDGAVRSWW
                     ARNPKLKHIHQRGIEALARHFGAGMETPFQELPQAFKDALLHGTGREAIPTGWAVGGG
                     KRSLARPYEGLVSEALRLYQNAESDALRAQVARFMNPLPCPECGGRRLRPGMLAVRLR
                     ARTEAGGLDSKDTPGSLSIHDFCSLPIRAAAAWMRGLESTGHQAGPVAELRAEVLKRL
                     EFLEQVGLGYLALNRESGTLSGGEMQRIRLAAQIGAGLSGVLYVLDEPSIGLHPADTA
                     RLIRTLLHLRDLGNTVLVVEHDEAVMRAADHIIELGPAAGEHGGRLIAQGGVGEVMAV
                     TESLTGAYLGGRLRITPPAPPLAPETGGQLPLPAGAGDHGAGFLTVHGASEHNLQNIT
                     AAFPAGRLTVVSGPSGSGKSTLVNRVLTRALMRHFHRAKDEPGRHQAITGIEHFDKVV
                     LVDQAPIGRSPRSNPATYAGAFTPIRELFAQLPLARVRGYDAGRFSFNTPGGRCEKCQ
                     GDGQVKIDMHFLPDAHVTCDQCQGRRYNAATLEVTFKGRSIADVLDMTLSEAARFFGK
                     AGAIAGKLRTLEDCGLGYLRLGQSGATLSGGEAQRVKLAAELARRPTGRALYILDEPT
                     TGLHFADIQTLLRVLFRLRDAGNTLIVIEHHPDVIRCADWVIDLGPGGGSEGGRVTAA
                     GTPAQILRDPASVTGRCLR"
BASE COUNT         4432 a         7238 c         7260 g         4337 t
ORIGIN      
        1 cacaaggcgt atccaaccag cgggctgaac aagcagcccc tgtgcaatct ctatgtgacg
       61 atgctccagc agttcggcgt ggaggtggag cggtttggga ttgggaatgg gcggctggcg
      121 gactttgggt gaccctgcct cttaatccgt gtcttaatcc ttgtcgtaat ctccaacctt
      181 tcgagccgga ttaagacacg aattttgaaa cacaaaccac gccatggctg aacggctgca
      241 tcacgaagac cttcgggtat atcaaaaggc tgtggctttt gtggcacagg catcggacat
      301 cctggaaccc gtttcctcaa agcatgcggt gaaagaccag cttcttcgtg ccgcggaaag
      361 catgccgctg aacatcgctg tcagcaatgc cagccagagt caggcaagtc agaagcaggc
      421 gcttgaaacc gccttcagtt cggcggctga gtgcgcggct tgcttggatg tgctgcagcg
      481 aaagcaactg attgcagggg atctttgcaa gactggaaag ctggaattgc aggaggtttt
      541 tcacatgttg atgggccttt ggaaatccaa agaggatcgt ctttgcgagg atgcgcctga
      601 gccgctttca acgggttttt cgcatgagaa gctggaatgc tatgggcgtg gtctccacct
      661 gatcggttgg gtgacggatt tttgtcacca gacccaggtg ccgcagcgca gccaggaggt
      721 gctggaccgc tccgtgacga gtcttgtgct caatttggcc gagggcaacg cgcggtgggc
      781 attgaaggat cgcgcacact tttttgattt gtctgtcatg gcaggactgc gatttgcagc
      841 gacgcaggat atccttgttg cgcggagttt ggcggggatt gagacagtat ccgaggcgaa
      901 gcgtgaagtt gcgattgctg tgcgacagat tttggggatc aaacgaaagg agatgctgtg
      961 atgaaatgga tgtttgagtt ggggattaag acaaagatta agacacggat tatgaagttg
     1021 gaaccggtca ttcttgttgt tatctttgcc ataatccggc caggattggc gaccgatccg
     1081 cgcgtcttcc tcaacgatta ctgcatctcc tgccacggcc ccgacaaaca aaaagccgag
     1141 cgccgctttg accagctcgc gctgccggca accaccgccg agcatgtcat tgacctccag
     1201 gacatcattg accagctcaa cctcggcgaa atgccgccga agaaatccaa acaacccacg
     1261 gatgccgagc gtagcgcaat ggcggcggct ctgacagagg tggtggcgca ggcacggtcc
     1321 gcgctgcgca gcacgggcgg gcagacggtg ctgcggcggc tgaacaaacg cgagtatctc
     1381 aacacggtgg gcgacctgtt tgcgctcgac atgcggcttt ttgatccgac cacgaagttc
     1441 cctcgcgacc agatggccga gcacatggac aatctgggcg atgtgctgca aacctccggc
     1501 tacctgctgg cgcagtatct cgacgcggcg gaccaggtgg tggagcgcgc gttcagcgtg
     1561 acgggcaagc cggaggaaaa gacctggcgt tttacagaca acttccgccc gcagcaggag
     1621 cacacctttc cgcacggcgc ggtgtacaag aaccgctacc tctgcctcta cgaggtgccg
     1681 gacacggaga accacgaggg cggctacggt tacattcacg acttcaaaca gggcgtgccc
     1741 gtggacggtc tttacgagat ccgcgtgaag gcccaggcca tgcatcgcga gcatccatat
     1801 gatccggcga tctttcagcg cgacactgcg cagcctttcc ggctcggcat cgtgccgggc
     1861 gatgtgaagg ccgggccgct gcatcatccg cagcccattg agccgcagct tgccgaggtc
     1921 acgctgaagg atggcgaggc ggaatggcac acgctgaaag tgccgctgct ggccgggcag
     1981 acaccgcgtt tcattttccc gaatggcatg gcgaacagcc gtcgcgcctt cggcaccctc
     2041 gcgcgcaaat atcaggacca atggcccgag aaggagcgca aggacctggg catcttccag
     2101 gcgcggcgcg tggtgctgca atacggcaag atgccgcaca tacgcattca tgaggtcgaa
     2161 atccgcggcc ccatcgtgca ggagtggccg cctgtttccc agcgcagcgt gctcggcgaa
     2221 gctggcttcg agccggagcg caggcgcgag attttgcagt ccttcgccag ccgcgcctac
     2281 cgtcgcccag ccacagcaga cgaggtggac cggctcatgc gtgtggtgga tacgcgcaga
     2341 caggcgggca gcacgccttt cgaggcgatg aaggacggtc tcaaagccgc gctttgctcg
     2401 ccggcgtttc tttatctggc ggaccctgcg gcgaaggagg agaagctcag cgcccatgcc
     2461 tttgccacac gcctctccta ctttctctgg tccaccatgc cggatgcgga gctgcgcgcc
     2521 ttggcggaca gcggcgagct tttgcgggat gaggtcaagc tcgagcaaac gcgccgcctg
     2581 ctcgccagcc cgcgcgcgga ggccttcatc gagggctttc tcgacgcttg gctgaacctg
     2641 cgcagccttg gcgacatgcc gccggatcgc gatgccttcg cgcgctttta cgccgatggc
     2701 ttgaagcccg cgatgaagca ggagacgagg ctgttcatgc gccatctttt ggatcaaaac
     2761 gaaaatcttt atcgcttcct cgacgctgat ttcactttcg tgaatcagcc gctggcggac
     2821 ctctacggcc tcggccgcat cagcccgccg gaagccgcgc aggagttccg caaagtcagc
     2881 ctcaccgacc gcaggcgcgg cggtttgctc gggcaggcca gcgtgctgac cgtgagcgcc
     2941 aacggcatcg agacctcgcc cgtggtgcgc ggcatctggc tgctggaaaa catcctcgga
     3001 acgccgcccc cgccgccgcc ggacaatgtg ccgcccattg accccgacat ccgcggcgcc
     3061 aaatccatgc gcgagatcct gaccaagcac cgcgacaaca ccggttgcta cgagtgccat
     3121 gcgaagatag acccgcccgg cttcgctttg gaaaactacg atcccatcgg ccaatggcgc
     3181 acgcattacc cggcgggcaa aaaacagggg ccgaagattg atgccagcgg cgaaatgccg
     3241 gacggcgcgc gctttgagga catcaccggc ttcaaggccc tgctcctgaa aaaggaggcc
     3301 cagttccgca aaaccctggc cgagcgcctg ctcgcctacg cctgcggccg ccgcatggag
     3361 ggcttggacc gcccgcaggt ggacgccgtg ctcgcggtcg tcgagaagga gggcaacggc
     3421 ctgcgcaccc tggtagagaa ggtggtgctc agcgaggtgt ttgccaggag gtgaagtttg
     3481 aaacgcattt cgatgccgcg cgtgtcagcc caccaggggt aaagcacgct tttgctgcgc
     3541 gcttagaaag gcggtgtcca cgatctgctg gccgatgcgg ctgatctcca gggtgagcgg
     3601 accgatgagc ggcgcgccgg tctccaggtg gtgaatgagg tgctcgatgg ggttgcggtt
     3661 cggggacggg atgacatcca cgggcacttc aaaaccctcg ggccgcgcgc gtgtctgcac
     3721 gcggatgtgc cgggcgtagt catagcagga gatggtgccc tgcgtgccgc gcaggacgaa
     3781 gccgcacatg ggctgcggtt gatgcgtcca gggatcggtg aaggtgcccc agcgcgtctc
     3841 cgtcttgctc aggccgcagg tgtaacgcag cacggccacg ctgtgctcgt ccacttcgat
     3901 gccctgcggc acattccagg tgcaggtcac ctccagcggc gctttgccgc ccaggtgcca
     3961 ggtgccgagc gtggcaccgt agcccatgta gtcgagcagc gagccgccgc cctgggccgc
     4021 gctgtaaaac caactggcag cttttccttg gtggagcagg tggtgctcag cgaggtgttt
     4081 gcgaggcttt agcggggatg acgtccgtca ctccaccgtc acgctcatgg ccaggttgcg
     4141 cggcttgtcc acatcgcagc cgcaggcgac ggcggtgccg ttttgtccac aaaactcgtc
     4201 ctttgcacac atgacgaccg aggtagaatt tcgattttcc catgacttca tccaacaagc
     4261 ccagcattga aatccccggc catctcctcg cttcctccag gcctggttat gactctgggc
     4321 gaaaccatcc cgtttcccgt gatgccgtcg agtcatggat caacgtcgca cgcgaggcag
     4381 gtgcggcttc gatcttgtgc ctgctggatg aacgccacct tgtgctctat gaagaggcgc
     4441 ttcctggagg tctgttggcg cattatgagc agtcgggttt tgatgtcggg cacctgccca
     4501 tccgcgacca tgaatggccg ccggtcaccg aggatgagct ccggcaggcc caggcatggt
     4561 ttgagaagct ccccaagccg gtgcttgtgc attgcagcgc aggccaggat cggactggag
     4621 ccgtggtgga gcacctttgc gctcttcatg aaaatcaagc caagccatcc actgacaccc
     4681 tggcctcccc atgaaaaaca acttcttccg tgagcgctgg ctcaagcgcc tcggtttgcc
     4741 ggacgctgat tggcaggagc ctatgcaaag gcatttggaa agtttgccct tcctggatgc
     4801 ttccgaaaaa aaagccgccc gtgcgatgat cctgtggctg ctggaaaaat tgcctgcccg
     4861 gctgcttcgc gatccaacgg agagcacaca gcgcctcgcg gaagcctttg ggtgcgcctg
     4921 cctggccttt tgacagtgtg gctccgcctt tccggccttt ccgcaaaact atgccgtgca
     4981 ccttcaggct caactcaagt tgcctgcggc caaaaggcag cctggaaccc agctcttggt
     5041 cagcctgctg cttgatgcct cgactgatgg agcctgcggc ctgaaccgcc tggaactggc
     5101 cgatgccgaa gtggtccgcg cttccgagcg gctcatcggc gaggggcgtt ttgaggatta
     5161 catcaagctg cctgaaaagt tcgccgagta tgacacacgc ctgcgcgagc acagaggttt
     5221 caaacatgac tgggaatgtc tttgccagca atatcccgct caagcagctg ccaccggcat
     5281 cctacaccgg agactgatcc cggaacggaa ctgggaacgc ggcccaggcg ctgaattcac
     5341 caacgaagac cagtgttttc aggccgcctt cgatctcttc tgctggaagt actacctctg
     5401 gggcgtgaaa gacggtgctc cgctgctgtt gaagccttcg gtggtcttca ctccttttgg
     5461 cacacagatt ttcatccctg gctacatgag ctttgatgcc cggcgcgacc ttgattttcg
     5521 ccgcatcaat gcgctgcaca aggcgcgtgg tgtcacacgt caggggccag ctttttcggc
     5581 tgggcgcatc gaaacagtgg agaagaaaaa gcgcgtcaaa gccgccaaga agcaggccat
     5641 tcaaaagggg ctgaaaggcg aggcacgcta cgactacatc agccagaaga gcggcatacg
     5701 cactcagggg gaccaccgca gcctgcgtcg gctggctgag tgacttggtg ccaggtttgt
     5761 ccacaaactt tgcccatgat gcgtcaggct tcaccagtga ggtttgagcc ttcaactcaa
     5821 cctcccattc ctatgagcca gcgcctcttc gaaatcagct acatcctccg caactccagc
     5881 aactccgcca cccagcgcgc ctacgtcaac gccagcgaca ccgtcatggc ccgccgcatc
     5941 ttcgaacagc aaaacccagc ttgtaaaatc gtctccagtc ccaaggatgt gacggaccag
     6001 cggtgaagac cgtcacagtc ggatcgtttt acatgactgc cagaccatga ctccctttga
     6061 atcactcctg cataaacttc agccacgaca tcgtctcatc ggagcggttg gcttgctcct
     6121 gcttctgttc ttggcctggt cggatcaggc accggctcag cagggcaaat ggctcgacta
     6181 cggcagcggc atgctgaacc tggcggtcgt caaatccatc ggcccctctt cccgaaacca
     6241 cctcaccttt gggtcccctc caggctatct ggagtttgac ggcatgcggc tggttttcca
     6301 atccccagcc gagcaggact cagcgcttcg agaaattcga agcttcctcg tctgcgggga
     6361 ttcctaccac aggctgcagg ctgaactgaa aggtgacacg gcaccatgag ttgattccat
     6421 tctcttcacg caccccatga aatatcccga cctcgtcaga tctgtccaaa agcacaaccg
     6481 aaactgggtc tgtgaaagct ttcctgaaga caaaggtgtt tggagtgagg atgaaagctc
     6541 catcaaaatt cacggtgctg tccatattgc tgactgtagc aatagcctgt gtggtttcac
     6601 caagaccgcc gatgatgtga cacaactggt ggagaaatgg ctcaccttga cggatcggaa
     6661 caagcaagag tgccgagtgg cagtcgccag tgtcgtggac aagggaaccc gtgattcaat
     6721 ggatgatctt cccgcagtgc ttgctgtagg catcaactat ggccagggag actggtatgc
     6781 agatcatgca gtgccgttgg aggataatac gcagttgaga agccaagcct caaaagtact
     6841 tcaagaactg aaacaaagga gctgctgcct ggatgaaatg gccagcttcc accttgtggc
     6901 ggccaatttt tttccatgga ttactcagtg gacctggggt gatctgaact ccatcgagga
     6961 atccattttg ctacgtggtt ccggacaccc cgactctgtg ggccatgtcg tcggcctgat
     7021 tcagaagatc aggcctcaag tcgtcatgtt tcatggtgcc aacaacgccg ctccaggttt
     7081 gggctggcat gtgatggata gactttgcgg gccgagctgg aatcgcgctg actgcaaatt
     7141 tgtattctca gacaacttgg cacctccgaa ccgcgccatc tcaaatgctg tgatgacccc
     7201 atgccccgtg ggacgccaca atttgcctcc cggctttgaa gcatgaaaaa tcctccactc
     7261 tatcacgctg tcttttcctc atgccgccgc taccgctatg agtggcatta tcgctggggt
     7321 gaggggccgg tgtgcatgtt cattggcctg aatccatcca cggcggacga ctttcagatg
     7381 gacccgacgg tgcgtcgttg tgtagactat gtacagcgct ggggctttgg cgcgctctgc
     7441 atgaccaatc tctttgcctg gcgtgacacg gaccccgagg ccatgaagca gcagccgcaa
     7501 ccgattggac ctaagaatga tgcaacgctg aagcgcttgg ctgcaaaagc agatctggtg
     7561 atcgctgcct ggggttgcca tggcagtcat ctccagcgtg gcgatgctgt gcgtcagatg
     7621 ctgcccaaca ttcatgcgtt gaaggtcaac caggattgct cacccgcgca cccgctttac
     7681 ctgagcaaga agctccagcc tatctccttc tgatggcatg atcgcaggtc gcgatgcctc
     7741 gccccaagct tgagtgccaa gaactgcctt catgaacgat ctcgatgctc tgctggccgc
     7801 gctcgccgat atgccggaac ctcacttcgc cagtgcgccc gaacggcttc gcaacctccg
     7861 tgaatacctc acggccttgc agcagggttc gttttcaggc gattttctgg tgggtgatgc
     7921 ctgcctgtct caaagcgcct ccctaaccgg cattccattc accagtgagt ttctactgcg
     7981 tgccgatgct cacccctttc ttgactcttt gcgccctcgt ctgtgggttg agggcaggca
     8041 gttcgagcag gcatcctatg agttgtggat ggcgctgggg gacggccaag ctgttcctgg
     8101 tttgtggcag ctttttcatt tgaaagcggg ggatgatgcc gaagagtgtg aggcatcgct
     8161 tttccgccat ctgctgcgcg tgttgaagcc caggcgaatt tttgcctggg gcaaagatgt
     8221 ggcggatttc ctgggacgcc agtttccaga gctggaggtt agacccctgc ttgctcggag
     8281 gtcatcctgg catgaagagg ctttgcgctt ggagttggag tggaacgggg tcatcaaggc
     8341 aagccaggag gagtcgcagg tgtctcgcgg cagtgagctg ggacagcttt atctcaaagt
     8401 gctgccgcgc catgctattg cggacactgc aactgccagc cgagaagaaa gacaaaactg
     8461 cggtgtggcc gctgccggag tgctggatga tcaatgccgg ttcacttttt tccggcacgg
     8521 ccagacggcg gcgctggcgc ggatgcttca gcaagctcgg caggtcgtgg gctatgattg
     8581 ccttattttc gacttcgaac tgatccgcag tgagaccaag ttcaaacgcc cgcctgccct
     8641 agaccttcgc caggagatcg aagatgcgac agggaagcgg cttagcttcg agaatgtggc
     8701 ccgcactgtc cttggaatac ttccaggaag tactgatgag ttgatgcgcg tgctagtggc
     8761 tcaggcagac tgggagaacg tgagtctcca tctggaaacc tatttgacgc tgcttaccaa
     8821 gctgcatcat cagttgtcgg agtagggcac ggttgctctt gttcggcaga gtcggtgatt
     8881 cctgcgttgc ggtttcactc caccgtcacg ctcttggcca gattcctcgg cttgtccaca
     8941 tcgcagccgc gggcgacggc ggtgtagtag ctgaagagct ggagcgggat gacggtgagc
     9001 agggggctga ggtagctggg gcagtcgggg acgtagatca catcctccgt caggcgctcc
     9061 agctcggtgc ggccttcggt ggtgacggcg ataacggggc ctttgcgggc cagcacctcc
     9121 tcgatgttgc tgacgttctt gtcgaaaacg gtgtcgtcag gggcgatgaa tacgctgggc
     9181 aatggcgacg gcacgccggt ctacattgag tatctcgcgg gcaccttcgc gtttttgagt
     9241 tcggagactt tgagcctcac gatcaccggc tggcacacgg atgggtcggc ggtgctcagc
     9301 gccttcatcg tcgtgaacaa gacctaccgt gtggagttct ccgaaaacat gaaggactgg
     9361 acgcgcaccc ccgtgcgggc ggactcaccc acggcggagg tgttgacaac cttcaccgcg
     9421 acggacacgc gggagatcag cctgtactcc gccgcctcga tcggaaaacc ccatcttttt
     9481 taccgtatgg tgctggtgcg ctgaactccc ttggaagacc tcacgaatca tccttctcgc
     9541 agcgctgctg ccgctggcgc cctggccgag tggcgcacgg aatccttcgc gctcaaggcg
     9601 ggctggaacg ccatttatcc gcatgtggac tccagccatg ccacgctgga cacgctgctg
     9661 gcggcctacc cggatgtgca tgaagtctgg cgctggcagc cggagcgggt ggacccgcgc
     9721 gaacccggtg atgtgaatgc cccgcctgcg cgctttggtg gagcaggtgg tgcttagcga
     9781 ggtgtttgcg aggtcgtgag tattgaaacg catttcgatg ccgcgcgtgt cagcccacca
     9841 ggggcaaagt gcgctttagc ttcgcgcttt ggaaggcggt gtccacgatc tgctggccga
     9901 tgcggctgat ctccagggtg agcggaccga tgagcggcgc gccggtctcc aggtggtgaa
     9961 tgaggtgctc gatggggttg cggttcgggg acgggatgac atccacgggc acttcaaaac
    10021 cctcgggccg cgcgcgtgtc tgcacgcgga tgtgccgggc gtagtcatag caggagatgg
    10081 tgccctgcgt gccgcgcagg acgaagccgc acatgggctg cggttgatgc gtccagggat
    10141 cggtgaaggt gccccagcgc gtctccgtct tgctcaggcc gcaggtgtaa cgcagcacgg
    10201 ccacgctgtg ctcgtccact tcgatgccct gcggcacatt ccaggtgcag gtcacctcca
    10261 gcggcgcttt gccgcccagg tgccaggtgc cgagcgtggc accgtagccc atgtagtcga
    10321 gcagcgagcc gccgccctgg gccgcgctgt aaaaccaact ggcggctttt tcctccgccg
    10381 tgggttcctt gtcctgcttg tccgcgccgt ggcagagggg gccacgattg ccgccatgat
    10441 gatgaaattc cacgacctcg ccaatgacgc cctcctggat gagccggtgc gcggtctgct
    10501 ggccggcatc ccacacgaga ggccagttca cggagagcac tttccccgtc gggggcatgg
    10561 cggccatcat ggcatcggcc tcggccagcg agccggcgaa gggcttttcc atgaggatgt
    10621 gcgcgccgta gggagccact ttttcgaccc attcgccgtg tcgggaagcg gcggggcaaa
    10681 ggatgacgat gtccggcttc gtggcctcca ggcaggcgct gtagtcggtg aagacacgct
    10741 ccgcgccgag gccgaacgtc cgcgccgcgt tttccatgcg ctcggggaac tcatccgaaa
    10801 tgcccacgat ctccgccgcc ggatgctcgt gcgtttggcg gaggaggtcg cccatgtgga
    10861 agtgatcgaa attgatgcct gcgatgcgcc agcgtttcat aggttagggg agggaggaaa
    10921 tgcccggatc aggaaaaaaa ggaatgctcc tgcggcaagg cgaaaggcgg gaatgatggc
    10981 cggttgggaa ggtgtgctat gcgaggactt ttcgacactc attcacaggt tgttcacatg
    11041 ggggcaactg gctggctggc gtgcggattt ggataacaat gaagtgcctg ccaaaggcgg
    11101 accggttcgg gccgatgctg tggaacgagg cttttggcaa cgtggaacag gcgatgccgt
    11161 tgtcacacgc tcgaggtctc cggcaggcgg cttgtggaac gaaattgttt ccgtgcggca
    11221 ggcgtcgccc tatattgatt caatgcaaat gactcaaaat gaataaaata agatggtaat
    11281 gccggagttt gacgagggtt tttgcaagtc ccatgaaacg tgtttccctg gcatggacag
    11341 gctggcatgt gacaaatggc tcgcctgtcg gtcctttcgg aagaaaagtg gattgtgaaa
    11401 aaagtcggcg gggtttcgga atgcttgacg cctttgcagt gtgttggatt tcaacctcac
    11461 agcgcgctgg attcacggaa tgtcttaagc ttgggcacat tccggcgggg gttcttcaca
    11521 agcgcccgtt ttgcggctgc cggaaggcct gaaggtgttg attttgatcg ttccgctcag
    11581 atgcgcctcg acaatgcggc gggggccggg tttgttgaat tcatggctct cacactcgat
    11641 gacatattgc agtcggcgga cactcaccag gccacggacg tgtttttaat ggagggcgag
    11701 acgccgcgca tgaagattca cgagcagctc atgaccctgg gggagtcggt catgcagggc
    11761 ggggacatgg ccgccctctg gcgcgcctgc ggcggcgacc ctgagagcga caccgaccgg
    11821 gactccagcc tcgtttcgcc gagccgcacg cgctaccggg tgaacatgca caaggcgctg
    11881 ggccgcctgg gcgtggtgat gcgccgcatc aagtcggagc tgcccgtcct gtcggagctg
    11941 ggcgttcctg actggctgct gacccgctgg gcgatgcgcg cgcgggggct catcctcatc
    12001 accggcccca ccggcatggg caaaagcacg acaatggcgg ggctgctgca gtggctgaat
    12061 gaaaacacgg cgcggcacat cgtgacgatc gaggatccgg tggagttcat ctatggcaac
    12121 cagggctgca tgttcaccca gcgcgaggtg gggcgggaca cggcaaccta cgcgcgggga
    12181 gtgcgcgggg cgatgcgaca gtcgccggat gtgatcatgg tgggggaaat ccgcgacctg
    12241 gagacagccc tggccacgct ccaggccagt gaaaccggcc accttgtgct ggcctcgatc
    12301 catagcgaca gtgtggtgga tgccgtggac cgcgtggcgc accttttccc gccggaacaa
    12361 gccggtttgg gcctctacct gctctcccat caactcatcg gcgcgctttg tcaaaagctc
    12421 atcccgcgcg tggatggcgg ggtgcatctc atctgcgagc acctggagaa catgggcgcg
    12481 gtgaagccgt ggatcgccgc gcaggagatg ggcaaggtgc gcgagtacat gcagcgcggc
    12541 aacgacccaa actgccgcac tttcatgcac gatcttgtcc aggcttatga ggcgggcgtc
    12601 atcaccgagg aggaggcggt catcacctcc ggcaacgagg cggaattccg ccgcgcggcg
    12661 cggggcatca gttgagaaga ttgacatgaa agccccgccg cccgtcctca ccatcgccgg
    12721 ctccgactca tcctgcggag ctggagcgca ggcggacctg aaggccatca ccgctctcgg
    12781 cggctacgcc ctcaacgcgc tgaccagcgt ggtttccgag acgcccggac gagtctcgaa
    12841 ggtgtgcctg ctcgaaccgg gcatcattgc cgaccagatg cgcgtgcttt ttgagggttt
    12901 cccgattgcg gcggccaaaa cgggcatgct cggcggcaag gcgcaggtgg aggcggtggt
    12961 ggaaaactgg cgcgcgcttg cgccaccggg actgcctctg gtagcggacc ccgtcatggt
    13021 cgccacaggc gggggcaaac tgctggagga tgatgccatc caggctgtgc gggaactgct
    13081 gctgccgctg gcagcgctgg tgacgccgaa catggacgag gcgcgcgtgc tctgggggcg
    13141 cgatgtgcga acgcgcgcgg acatggagcg ctgcgcccgg gagctcgcgg atgtgttaca
    13201 aaccagcgtg ctcgttaaag gcgggcacct ggagggcggc gcggcggctg atgtgctgtg
    13261 cgcggatgga acgatcacct ggcatgagac ggaacgcgtc cctggagtgc ggacgcatgg
    13321 cacaggctgc acctactccg ccagcatcgc cgcgtgtctc gccctggggc tgcccttggc
    13381 ggatgccgtt gcgcgcgcga aggcgtatgt ggccggcgcg atcggcggct ttttgtcatg
    13441 gcagggagcg cggggggagg ttcacgcgct gcaccacttc gcggggagac ttcaggatgc
    13501 cagttgatgc ggtttttcca gcagtagcac gcgaaaagta cggcctagaa aggccggatg
    13561 cgtgagcgtg tggtactggc gcagcagtgc ccgtgtggcc gcgtccggcg gcgcgccgtc
    13621 gagcgagcgc agccagtcca ttccgagcct gccaaggaat cgcccctgat gctcgtaagc
    13681 gagtgttgac agtccgcgac tagcggctgt ctccgcgagg cgggtgaagt tcacatgcgc
    13741 cgtcagatcg cagcggccca aatcctccag cacgcggtca tcactctgat gggaatggta
    13801 gcggcgcagc gtgccgcctg cgcgttcggg agaaaaaaat tcctcctcgt ccagcccata
    13861 gtccgcgatg aaaattcgac cgtgaatcgc cgaacgcgcc aacgcctcca tccagtcgag
    13921 cgcggcgagg ccgatctcca tcatgtgccc ctccggcagg ttcacaggca ggcgggctgc
    13981 ctctccgcgc agacgcccgg aggaaagcgg accgctcttg aaaatcgcgc gcccgccctc
    14041 ccatgccaca tggagctcgc gccattcccc ggattcaaaa cgcagcagat gctcgggcat
    14101 ggcgtccggc agctcattgc agacaaaaag ggcggggcag gggggcagtt catccaggct
    14161 gtccgtccat gaaacacgat cctggaaatc cgccagacgc gcgtgttgcg ctgcctggta
    14221 gcgcggattc ggttccacaa tccgcagggg cagcccgcac gcggagagca tgtcatgggc
    14281 gagttggccg tcgtgcgcgc cctgctcgat gatgcaaaat tcccccggct ttcccatttc
    14341 attccatgtt ctttccgcca gcccggccag cagcctgcca tagagctgtc ccgcgctcac
    14401 ggcagtgaaa aaatcccccg cccgtccgat gcggcgggga gcgggtccgt agtagccgtg
    14461 ctccgggtgg taaagcgcca gctccatgac gcgcgcgaag gtcaggcgcc cgtcgggggc
    14521 tgcggaggct tcggcgatga gaatgtcatc cagtatggtc atgggaaagc gctggcggca
    14581 ggcgcgtgag gcattaaact ggcccggctc tgacgcaaga gcgactgatc atgtttggag
    14641 acgacgacct gcaagacccc tcgaagaaaa actggcgcgg cgagcccgcc ctcaagccgc
    14701 gcttttaccg ccgcgcctgg ttcagcgcgc tcatggcgct gatcctgctc ggcggcgtct
    14761 ccctgctcgg cgtctgggcc atcgtggtcg cgcccttgaa ggaaaaggcc gaggaatacg
    14821 acctggacga ggtgcgcaag ctggagtcgg ccagcatcat ttatgaccgc aacggcgagg
    14881 agctctcccg cattttcgtg ctcaaccgca cgccggtgtc catccaggag gtgcccaagc
    14941 acctcatcaa cgccctcatc gcgcaggagg acagccgctt tttccgccac aacggcgtgg
    15001 actacatcgg ccttgggcgc gcggtgatcg agaacttgaa ggccggacgc accacccagg
    15061 gtgccagcac catcactcag cagctcgcgc ggcagacctt cgggttgatg gaaaaaagct
    15121 acaagcgcaa gctcatggag gccttcgtgg cgcagcgcat tgagaaaaag tattcgaaga
    15181 ccgaaatcct ggagctttat ctgaaccgca tctttttcgg caaaaacttt tacggcatcc
    15241 aggccggcgc gcgcggctac ttcggcaagg acaccaagga cctgactatc gaggaagcgg
    15301 ccaccctggc ggcgctcatc aaaagcccga acaacatcca gccgctagat catccagcgc
    15361 gggcggtgaa ggagcgcaac tatgtcttcg accgcatggt ggccgagggc ttgctcgacc
    15421 gcgtcgaggc cgagaaggcg aagatcaaac ccatggtcac cgcgccccag ggcaatgatc
    15481 cgcgccttag ctacgccttc gatgaaatcc gcgcccaggt catgtcaatc gtcggcgagg
    15541 agcgtgccgc cgttggcggg ttcctcatct acaccagcat tgacaaggat ctccaaaacg
    15601 ccgccgaagc ggccctgaaa aagcggctgg ccgaagtcga ggcccgccca ggctacgccc
    15661 accagaccta tgaccagttc aaatccatcc tgcgcgactg gagagccggt ttgtccaagc
    15721 ttgcggcgga cgcggaggcg cctccgcgcc ccattcccga gtaccttcag ggtgcggccc
    15781 tggtcattga caaccgcgac ggcgccgtgc ttgcccttgt cggcgggcgc gactttgtgg
    15841 acagccagtt caaccgcgtc agccagggat cgggacgtcc cacaggcacc tcgttcattc
    15901 cctttgtgta tgcggcgggt ttttccaagc ccgacttctt ccccatgtcc cagctcgccg
    15961 acgagcctct cggcaatgac cgcgtcatga tcggcggctt caccggcatc ctcggcgaat
    16021 acggaatgga agtggccgat cccaaatggt cgcgcaaacc catcagcgcg cgcgaggcgc
    16081 tggtggcctc gcacaattcc gccaccgttc gcctgggcga gcgcgtcggt ttggagcgcg
    16141 gtctcgtcac gccgcagttc agggattttg tcaaaaagac cggcatcaac gcgcccttgc
    16201 gcgacggtcc tgccaccttc ctcggcgcca gcgaggcccg cctggacgaa atggtgctcg
    16261 cctactccag cttccccacg ctcggccgcc gccccgccaa gctgcacatc atccagcgcg
    16321 tcaccgacgc ggaggaaaag accgtctatc aggtgccgga ggagtctctg cagaccgtgc
    16381 gcgccatgga tgagatcgcc gcctggcaga cccacacctg cctcgatgag gcgctgcacc
    16441 gcggcacagg ctcccccgcc gtcagcgagt atggccttgg cgacttcccc gccgccggca
    16501 aaaccggcac ccaccacgaa tacaaggacc tctggttcct cggctacacc tccgctgtca
    16561 cctgcggcgt ctggaccggc tttgaccgcc agcggaccat ccacgacatg gccttttcca
    16621 atcgcatcac cctgcccatc tggacggaca tcatcaacgc ctccattaaa actcacgcac
    16681 cgcaggactt ttcaccgccc gagtccgccg agcgcattga gatatgccgt gcaagcggat
    16741 tgcgcgccac cgacttttgc tatgaaaaag ttcgcggttc cgacggcgtc gagcgctccg
    16801 tgcgcaacac ctacatcgag ttcgtccgcc ccggcaccac ctacaacgcc acatgcgccg
    16861 tccacaccgg cgaagggctg cccgccgatc tcgccgcctt tcaccagacg ctaacccaat
    16921 cctccgagtc gccgctgctc gccgatgcgg gcaggtttgt gaatgtagaa cccgtccaca
    16981 tgcgagatcc tctcatcatc ggcgctgatc cctacaattc catcatgccc gttttgcgcg
    17041 cccgcccggt caatgacgac gggagcccga ttctgcgcgc cctgccagta ggccccgatg
    17101 aaggcggcgc tcctgaggaa gcccctgtga tccgcctgaa accgccgccg ccgatgaaga
    17161 tcgagctttg acgcgctcag agtgaatgga gcgtggacgc tgcccgacga ttctaaaagg
    17221 caggtcggac attttttgtc cacggcattc attatcccat gcacgcgcca gcagcggcag
    17281 gcaggatgct tgtcggagtg gcaaattggc tccagaaggt gaggccaggc ttggcgggcg
    17341 cgtcgccaga gtgcccggcg acgaactcgg acgcggcgac tgcatcggtg ctggaaagtt
    17401 gagtgcggga gccaaaagca ggcatggaat tccagccaag gtccatttca atagcgggtg
    17461 tgttgagcgc gttcattttg agaatcgaag gtgagtttgt gagatgacgg ttttgagaat
    17521 tatcggaatt aaaaggtctt gtttgcgact ttgaatggct gcggtgagat gagacgggtt
    17581 gttgagtcgc gaaaatgaga atcacaggcc agcaggatgc ctgggtgtaa agcctgcata
    17641 gctgcggccc ttggtgcgcc agcgtggacc ttctcccact tggccaccgg atgtgtccag
    17701 acgaagaccg cagatggtca tgaaaacatg ctcaccgggc ttcacccaga ttgtgatgaa
    17761 gcggccctca cccggatgtc catagttggc aaagccagca gaagtcaggg gagtcttcag
    17821 cagacccgcc ttgatcagcg catacgaagt cgaactggaa cagtcataag cgcggtcttc
    17881 gacccgtcca tgaccggcgc cgcgcacata ggggctgctt tgcaggtggt tggcggcatt
    17941 gatcaagtat tggacctggg tgggcaggct tgcggagcag gacgcatact gaccgtcgaa
    18001 acgaatcaag gatccggtcg cgcggggggc cgccaccggg gttgcacgcg gagcgggggc
    18061 gcgttgtgcg gccattgcct gctgctgggc gtagtaggcg gcccaagctt gctgattctg
    18121 cacatacgct tgatatgcgg cctgctgctg ggcgtaagcc gcctgctggg cagcgtagtg
    18181 ggcggctgca taataaccgt tctgcgcggc ggcttctttg gaagcggcgg caaacaccag
    18241 agtggcaaga gcgatgagga tttttgaatt tgttttcata gcagcagttt cggttgctat
    18301 ggaaattata ttcaaattct agaatcatcg caattgaaat ttcataattg attcaatttt
    18361 ttgtaacaag tttcgaaaaa tgcgtgattt gagaccaaag tcacaacaaa gaccaatcaa
    18421 aagaacagtt tttatgttat cacattcgag tttgatggtt tttgaatgtc tcaaaattct
    18481 caaattggcg gcgattgttc taaaatttct caattctggc atcctgatgg gccagattta
    18541 aatcaggcca cctctgtgat gtgcgaggtt gccgggcttg aggggggggc agttgaaaag
    18601 tgacgagtac cgaaggctgc ggcaaaaccg ggccgcatct tgaccgtatg cccctggaac
    18661 attcggcggt aaggcggcgc tgttttttga aagcggcaaa ctggcggatg aggttggcgc
    18721 gccgcagtcg cgcttgaaat ggcaggtgcg gcgggcgctt ggaaggagcg tcagagcgat
    18781 ggcagtgtga tttttctgca agtctccgcg agagggcgcg tttttgcgcg gctgaaaccc
    18841 cattcccatc gtcatgaacc ccacttccac tccctttggc cgccgtcagt ttatcacggg
    18901 cgccgcgcag acttttctcg gtgtcaccgc catgagcacg ctcgcaggca aggcgctggc
    18961 cgccggcaag gatgccagca ccgcgcgcca ggcggccacg gcgcgcaatg tgatctacct
    19021 ctacatgaac ggcggcatga gccatctgga cacctgggat ccgaagcccg cgagttccgg
    19081 cgtcatgggc ctgaccaaga cgatcaagac gcgcgcggat ggcattcaga tcagtgagaa
    19141 cctgcctttg ctggcccggc agacggacaa gctggccatc atccgctcca tgtcctccac
    19201 ccagggcgct cacgagcagg ggaactactt catgcacacc agctacacgc tgcgcagctc
    19261 catccgccac ccctcgatgg gcgcgtggct gcaaaagttc caggatcgag gcaaccccac
    19321 gctgcccggc agtgtgatga tcggcaacga cagccgccac cccggcgcgg gattctttga
    19381 gtcccgcttc tcgccgctga tgatcaacaa cccggaaagc ggcctggcca acgtgcgcgt
    19441 caacgactgg ttcaccgagg agcgcttcag cagccgcctg gaggtggcca aaaagctgga
    19501 ccgcaaattc gccgaaacct acgacgtgaa aaacgtccgc gcctacagcg acatgtatga
    19561 cgacgcggtg aagatgatga agagcgagga gctgaaggcc ttcaatctcg cggacgagtc
    19621 cgacgcgctg cgcgagaaat acgggcgcga ccgcttcggc cagggctgcc tgctggcgcg
    19681 ccgcctggtg gagcacgggg tgcgctttgt cgaggtcagc tttggaagct gggacacgca
    19741 caatgccaac ttcacccgcg tgcccgaact ctgcgaggag ctggacaacg ccatgagcgc
    19801 cctgctggcc gatctggaaa gccgcggcct gctggaggag accctcatcg tgctggccac
    19861 cgagtttggc cgcacgcccg agatcaatgc caacgacggc cgcgaccatc atccggcggc
    19921 cttcagttgc gtgcttgccg gcggcggagt gcgcggcggc caggtttacg gatccacgga
    19981 cgagaaaggc gccaggatca ccgggaatcc cgtcaacgtg cccgacatca acgccaccat
    20041 cggctacgcg ctcggcctgc cgctcgacca ggtgctctac tcgccctcga agcgcccctt
    20101 caccgtcgca gacaaaggca agccgctgac caccctcttc ggttgaggct tcggacgcgc
    20161 tcagcgacgg gccgtttgca gccgcccctg gcgcatggca cgggcatgcc cggcgcccaa
    20221 gcgcggggtt tgacgccggt ggcatccccg gctttcaatg gcggcatggc gcaggacctc
    20281 atccgggtgc gcggagcccg gcagcacaac ctcaagaaca ttgacgtgga catcccgcgc
    20341 caccggctgg tggtgctcac cggtgtcagc ggcagcggca agtcctccct ggccttcgac
    20401 actctctacg ccgagggcca gcggcgttat gtgcagagcc tctccgccta cgcgcggcag
    20461 ttcctcgacc agctcgaaaa gcccgacgtg gacttcatcg aggggctctc ccccgccgtc
    20521 gccatcgagc aggcccgcgg cgcgccgaac ccgcgcagca ccatcgccac cgtcaccgag
    20581 atctacgact acctgcgcgt gctctacgcg gtggccgggc tgccgtatga tccgcaaacc
    20641 ggcgcgccct tgagcaggac cacgccagcg gagatgggcg cgcgcatcct ggagctgggg
    20701 gagggcgcgc gcgtggtcgt gctggcgccg cagccgccgg cggacgcgga cgggttgagg
    20761 gcgctttttg aaaagctgcg ccgccaggga ttcgtgcgcg tgcggctgga cggcgtcatc
    20821 catgagctgg acgagccgcc ggagttggag caggggcgga cctgcgccgt cgagatcgtc
    20881 gtggaccggc tggtcatccg cgagggcgcg caggcgcggc tcatggagtc catcgaggcc
    20941 gccctgcgct ggaatgagaa cgaggcgcgc ttcctggtcc aggcccgcgg gggaggggag
    21001 gagacactct cattcaccac cgcctatgtg aacccggaga cgggctacac gcttggcagg
    21061 ctgacaccgc agcacttctc cttcaacacc cacctgggcg cctgcccgcg ctgcgagggc
    21121 gtgggcgcgg tcatggcgcc cgaccccggc ctcatcgtgc cggacgcgcg gctcagcctc
    21181 aaagacggcg ccgtcaggag ctggtgggcg cgcaatccca agctcaagca catccaccag
    21241 cgcggcatcg aggccctggc gcggcacttc ggcgcgggca tggagacacc cttccaagag
    21301 ctaccgcagg ccttcaagga cgcgctccta cacggcacgg gccgggaggc aatccccacc
    21361 ggctgggccg tcggcggcgg caagcgcagc ctggccaggc cctatgaggg cctggtcagc
    21421 gaggcgctgc gcctttatca gaatgcggag agcgacgccc tgcgcgccca ggtggcccgc
    21481 ttcatgaatc cgctgccatg cccggaatgc ggcggcaggc gcctgcgccc cgggatgctg
    21541 gccgtgcgcc tgcgcgcgcg gacggaggcc gggggcctgg actccaaaga cacgcccggc
    21601 agcctctcca tccacgactt ctgctccctg cccatccgcg ccgccgcggc gtggatgcgg
    21661 gggctggaga gcaccgggca ccaggccggg ccggtggccg aactgcgcgc cgaggtgctc
    21721 aagcggctgg aattcctcga acaggtcggc ctcggctacc tggcgctcaa ccgcgagagc
    21781 ggcaccctct ccggcggcga gatgcagcgc atccgcctgg ccgcgcagat cggcgccggc
    21841 ctctccggcg tgctctatgt gctcgacgag ccgagcatcg gcctgcaccc cgccgacacc
    21901 gcccggctca tccgcaccct gctgcacctg cgcgacctgg gcaacaccgt cctcgtcgtc
    21961 gagcatgacg aggccgtcat gcgcgccgcc gaccacatca tcgagctcgg gcccgccgcc
    22021 ggggagcatg gcggcaggct catcgcccag ggcggcgtgg gggaagtcat ggccgtcaca
    22081 gaaagcctca ccggcgcgta tctcggcggc cgtctgcgca tcaccccgcc cgcgccgccc
    22141 ctggcgccgg agaccggcgg gcagttgcca ctgcccgcag gggctggaga ccacggagcc
    22201 ggcttcctca ccgtccacgg agcctccgag cacaacctgc aaaacatcac cgccgccttc
    22261 cccgccggcc ggctcaccgt cgtcagcggc ccctccggca gcggcaagtc caccctcgtc
    22321 aaccgcgtgc tcacccgcgc cttgatgcgg catttccacc gcgccaagga cgagccgggc
    22381 cggcaccagg ccatcaccgg catcgagcac ttcgacaaag tcgtcctcgt ggaccaggcc
    22441 cccatcgggc gcagcccgcg cagcaacccc gccacctacg ccggcgcctt cacccccatc
    22501 cgcgaactct tcgcgcaact gcccctggcg cgcgtgcgcg gctacgacgc gggccgtttc
    22561 agcttcaaca cccccggcgg ccgctgtgag aagtgccagg gtgacggcca ggtcaaaata
    22621 gacatgcact tcctgcccga cgcccatgtc acctgcgacc agtgccaggg ccgccgctac
    22681 aacgccgcca ccctcgaagt caccttcaaa ggccgcagca tcgccgacgt gctggacatg
    22741 acgctcagcg aggccgcccg cttcttcggc aaagccggcg ccatcgccgg aaagctccgc
    22801 acccttgagg actgcggcct gggctacctg cgcctcggcc agtccggcgc caccctctcc
    22861 ggcggcgagg cccagcgcgt caagctcgcc gccgagctcg cccgccgccc caccgggcgc
    22921 gccctctaca tcctcgacga gccgaccacc ggcctgcact tcgccgacat ccagacactc
    22981 ctgcgcgtcc tcttccgcct gcgcgacgcc ggcaacaccc tcatcgtcat cgagcaccac
    23041 cccgacgtca tccgctgcgc cgactgggtc atcgacctcg gccccggcgg cggtagcgag
    23101 ggcgggcgcg tcaccgccgc cggcaccccc gcccagatcc tgcgcgaccc cgccagcgtc
    23161 accggcaggt gcctgcgctg agacagggcc aaagcccccc tcaaagcccc ctgccggcct
    23221 cacccgcacc gtccatctgg aagccaccgc ccttatcaac caagccc
//
LOCUS       DAOMYJ010000066        22942 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Prosthecobacter sp. isolate AMR_MDS_5629
            NODE_808_length_22942_cov_23.422336, whole genome shotgun sequence.
ACCESSION   DAOMYJ010000066 DAOMYJ010000000
VERSION     DAOMYJ010000066.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867850
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prosthecobacter sp. (wastewater metagenome)
  ORGANISM  Prosthecobacter sp.
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Verrucomicrobiaceae; Prosthecobacter.
REFERENCE   1  (bases 1 to 22942)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 22942)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.30
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:34:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,742
            CDSs (total)                      :: 4,684
            Genes (coding)                    :: 4,676
            CDSs (with protein)               :: 4,676
            Genes (RNA)                       :: 58
            tRNAs                             :: 55
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22942
                     /organism="Prosthecobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_808_length_22942_cov_23.422336"
                     /isolate="AMR_MDS_5629"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1965333"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..325)
                     /locus_tag="PLP58_08980"
     CDS             complement(<1..325)
                     /locus_tag="PLP58_08980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ08688.1"
                     /translation="MSTKLYLGTRKGLIEAVRGTNGWEITRMSFLGVQVPMLLPDARD
                     GTLYAAVDHGHFGTKLHASRDGGANWEERACPTYPPKPDDYVEVVNPMSQEPTPWSLK
                     KIWALE"
     gene            complement(340..609)
                     /locus_tag="PLP58_08985"
     CDS             complement(340..609)
                     /locus_tag="PLP58_08985"
                     /inference="COORDINATES: protein motif:HMM:NF014636.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoaD/ThiS family protein"
                     /protein_id="HRJ08689.1"
                     /translation="MPTVEFTANLANQTHAPKCRVDGRNVRECLDAVFELHPKLRGYV
                     LDDQNAIRQHVVVFVDGTAIRDRRAQTDAVRPDSELFVMQALSGG"
     gene            complement(622..984)
                     /locus_tag="PLP58_08990"
     CDS             complement(622..984)
                     /locus_tag="PLP58_08990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009962087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1428 domain-containing protein"
                     /protein_id="HRJ08690.1"
                     /translation="MPTYTDGFLLPLPKKNLAAYQRIARQAGKLWKEHGALDYRECVG
                     DDLKNDWGTLFTQSAGSKPSEVVIFSFITYKSRKHRDQVNKKVMADPRIHKMCDPSKM
                     LFDCKRMAYGGFKTIVQA"
     gene            complement(998..1432)
                     /locus_tag="PLP58_08995"
     CDS             complement(998..1432)
                     /locus_tag="PLP58_08995"
                     /inference="COORDINATES: protein motif:HMM:NF019928.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HRJ08691.1"
                     /translation="MNPAADNEILSSRVFPHSRQRVFAAFSDPKQLTLWFGPKGFTST
                     FETFDLRIGGEWKFIFHGPNGANYPNHTVFADIVPLERIVYDHVQPHFRMTMTFADEN
                     GSTRLTWRMTFETVQLSETLKGICAPSNEENFDRLAAHLASI"
     gene            complement(1429..1854)
                     /locus_tag="PLP58_09000"
     CDS             complement(1429..1854)
                     /locus_tag="PLP58_09000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007418009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HRJ08692.1"
                     /translation="MSAPFIQPYLFFGGRCEEALAFYKTAIGAEVEMLMHYSDSPEPC
                     APGMIPPGYENKVMHTSFRVGSSVIMASDGCGDTSPFAGFSLSITVATEAEADRAFNA
                     LAEGGEVTMPLAKTFWSPRFGMLKDKFGMGWMISTEVCP"
     gene            complement(1874..2356)
                     /locus_tag="PLP58_09005"
     CDS             complement(1874..2356)
                     /locus_tag="PLP58_09005"
                     /inference="COORDINATES: protein motif:HMM:NF019928.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HRJ08693.1"
                     /translation="MSLPAPIESPLSERELVLVRETDVPREKLYADWTTAELYPQWFC
                     PKPWTVADVELDVRAGGFSRMTMCGPDGERFPNQGVYLEIVPNEKIVFTDAYFANWEP
                     NPNPFFTGIITFETLPDGKTRYTARALHWTKENSEKHAAMGFHEGWAKAFDQLVEICR
                     "
     gene            complement(2386..2922)
                     /locus_tag="PLP58_09010"
     CDS             complement(2386..2922)
                     /locus_tag="PLP58_09010"
                     /inference="COORDINATES: protein motif:HMM:NF022072.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HRJ08694.1"
                     /translation="MIKKILLGLVAVIAIILVVASFQSDDMTVSRSTTIPAAPEAVFK
                     VVNDFRLWDAWSPWSKLDPNMKKTLEGPPEGVGAIYKWSGNDEVGEGTTSLIASKPNE
                     EIGMKLQFVRPFEGDADVKFTFAAEGTGTKVTWTMQSKKPFIGKVVGLFMDCEKMCGD
                     QFNEGLANLAKVVAAPKP"
     gene            complement(2956..3411)
                     /locus_tag="PLP58_09015"
     CDS             complement(2956..3411)
                     /locus_tag="PLP58_09015"
                     /inference="COORDINATES: protein motif:HMM:NF013098.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HRJ08695.1"
                     /translation="MNTPTTYPALSAYITVKEAAKAIEFYKAAFDAKELFRLTMGGPD
                     CIAHAELSIRDTLIMLSEEFPGMSKSPQTLGGSPVTFCLIVDNADAAFAQAVAAGATV
                     LRAVADQFYGFRSGCVTDPFGHIWMIQHQTETLTPQQMQERMSAMPQGS"
     gene            complement(3418..4044)
                     /locus_tag="PLP58_09020"
     CDS             complement(3418..4044)
                     /locus_tag="PLP58_09020"
                     /inference="COORDINATES: protein motif:HMM:NF028235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HRJ08696.1"
                     /translation="MPKKAASKKRERDPEGTRQALIDATVRLMLRQGFAGTSVDMICK
                     EAGMTKGGFFHHFESKEALGKAAVEWWGQMGASFYNAAWQNSAIGPLERLHQMLDIMS
                     GFTLRKDENCVCMVGMMSQELASTNPVMQAACAKELQSWNDNVAQMLADAKKQLKPAT
                     NFDPKAVAWFLNSLWQGSMLVGKAVDSQAMIRANLKLARTFVDSLFDK"
     gene            complement(4128..5684)
                     /locus_tag="PLP58_09025"
     CDS             complement(4128..5684)
                     /locus_tag="PLP58_09025"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arylsulfatase"
                     /protein_id="HRJ08697.1"
                     /translation="MRHLAFLLLSVLCVPLLAAKPNFVIILADDLGYGDMQVNDPERG
                     KIATPNMDRLAAEGMRFTDGHSSSGCCSPSRYTLLTGRYHWRTTLQSGIVGLWGKPLI
                     APDRLTIGKLAQQHGYRTACIGKWHLGSDWPVTPEQKVLLTGFGGKSGGGGDVSTTIT
                     DAHREAWKAVFSQRIPGGPSERGFDEYFGTDVPNWPPYCFIDGDRTVGIPSELLPASK
                     LVKNQASIQGPALADWQLEGILPALASRAEKFIQTQSAAKQPFLIYLPLTSPHTPLAV
                     NAEWKGKSGLNSDYADLVMETDQVLGRVLDALKTAGVENDTFVLFTSDNGCASYIGVK
                     DMEKQGHYPSGPLRDYKASVYEGGHRVPFVVRWPGVVKAGSVCKQLVLQADTLATMAE
                     ILGVKLPETAGEDSFSLMPLLKGEDRPIRTNAVNTACGGIPSYRDGAWKYVAAAEPEL
                     YNLDEDLAETMNMAPLHPERVKAMQASFEKLIRAGRSTPGAPQKNDIKVVRYPKEAAK
                     SKAKKAGGAK"
     gene            complement(5704..7146)
                     /locus_tag="PLP58_09030"
     CDS             complement(5704..7146)
                     /locus_tag="PLP58_09030"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arylsulfatase"
                     /protein_id="HRJ08698.1"
                     /translation="MKHFFSSSRLLSFLSSFALLVSPLFADTKPNFIVILADDLGWGD
                     ISCQGATKIQTPNIDRLAREGARFTDAHSPASVCSPSRYGVMTGLSPWRLGRKGNGYK
                     LKKDRLNIASMLEAQGYRSAAIGKWHLGYGKDWNQKRITGPLEVGFNYHFGVPGNHND
                     QYRVFIENHDIFGRKPGEAYRVVNDGRDLPEGVSQPRVEDQVDTTLTAKAVGFIREQK
                     DQPFFLYFTPCASHTHITPAAKFRGTSQAGLLGDYIQELDAHVGDLLATLDELKLSER
                     TLVIFSSDNGGTPNDFKGTHDMNLNLASEAGGVREKFHTAKQDARAMGHYTNGPWHDG
                     KGSPYEGGHRVPFIARWPGQIPAASTSEQTICLTDLLATFASIVGAKLPDDAGEDSFD
                     ILPALRDPSLATPIRPITILQADTKDDVLAVRSGRWKFTENKNGKKKTLHDLTQDVGE
                     TKDIAAEHPEVVKQLSAQLAQAREAGRTRK"
     gene            complement(7202..8497)
                     /locus_tag="PLP58_09035"
     CDS             complement(7202..8497)
                     /locus_tag="PLP58_09035"
                     /inference="COORDINATES: protein motif:HMM:NF024627.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HRJ08699.1"
                     /translation="MKQTMLLLVAVTSLFAPLTASAAKPPTATAVIQVVDFLPADHVT
                     DGSVSYQSAIQSAIDAVPGTGGTVVFAPITYRIDDPVGLRLRSNLTLSMEGATFLLSE
                     TCAADGQLFLGQGVSGLRLEGGTIIGRNDVWLTGVNIRGVHLTGECRDIRIRDMHIRD
                     LSSNGIGIFAADEEHPSTDVWITDTVIDNCANVYGDYQAESKRGPEKGSTREDQGLIA
                     LYHVHDFVVRGCRFEDSRSDGTHLYYCRRGQFTDNRIYRAKMGGYFLETCQHVLAANN
                     VMRDNGSRGVTIERKSDFCTLIGNTIEGSGREGLWIPDSARCVVTGNVFSLNGRKNNG
                     TERHHLWNPNITINEARGDKFATPTASYLIADNIIETDAHQLAAIRVDTREEVRDILI
                     QNNFLIGDNRSIRVEGPDQDAVTIEQNRGAKVDRIIDKE"
     gene            8592..9437
                     /locus_tag="PLP58_09040"
     CDS             8592..9437
                     /locus_tag="PLP58_09040"
                     /inference="COORDINATES: protein motif:HMM:NF020037.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HRJ08700.1"
                     /translation="MRNATPLQPRSRQSRRSLAEIQRWRARRAAWFSQLDAACGFQAL
                     FDHIPAVFFFAKDREGHLMFASKGLLGRYQMRDDSEFIGRTDFDLNPGSMAQAYVEDD
                     AKLLRGRAPAIERMELWWDRQGMPDWYLVTKLPLRDRRGRVQGIMGILRRADEHDRRL
                     PVFQTVSRAVEIIRRDFARPITIGEVAGICGESLRQLQRRFLIAFGIGPQEFLIKTRV
                     LAAARLLDESDLTAAEVATRCGFVDASSFTQQFKQRTGTTPARYGKRRTPTGPGPSAG
                     REPRG"
     gene            9505..10260
                     /locus_tag="PLP58_09045"
     CDS             9505..10260
                     /locus_tag="PLP58_09045"
                     /inference="COORDINATES: protein motif:HMM:NF015585.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialate O-acetylesterase"
                     /protein_id="HRJ08701.1"
                     /translation="MKHLALILLALCPLLSAFAADKPAHLFILTGQSNMAGMDPKLGF
                     EPEAKKLFPGADVAHIKVAVGGMPIRYWVDEWNDIAAKHGVDVAKAREADKRKGTIFY
                     PRILEQFAELLKQHPNAASVTFCWMQGENDSRTGLQAPYADALKQLIANLRRDLKRPE
                     MNVVIGRLSDHDELNPSAWKVVRESQVAVALADPHGAWVDCDDLNDKEVKGVMTNDLH
                     YTKPGYELLGRRFVRQAKALIEGGKPAENGRPE"
     gene            10314..11285
                     /locus_tag="PLP58_09050"
     CDS             10314..11285
                     /locus_tag="PLP58_09050"
                     /inference="COORDINATES: protein motif:HMM:NF012363.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HRJ08702.1"
                     /translation="MITHRFPLVLGLVFLVSLPAAAQQKARKETAKGPPPTFADVPYG
                     TDERQVLDFYQAKSGQPAPLVLFIHGGGWRGGDKDRAAGYGLNEYLEAGLSVAAINYR
                     FVPAAHEAGIKPPVKWPLEDAARALQFVRSKAGEWRIDKTRIGATGSSAGAASSLWLL
                     FHDDMADAGASDPVARESTRLTCAGVLHAQTCFDPHLLRAWMPNMTYGGHAFGFIKDG
                     QTRKDEFQAFHDGREQIMPWVKQYSPYHLVTADDPPVLLQYDARADEPPVVGKRAGDA
                     PHSAVMGIKLLEKLRETGVEGHLVYPGGPESSYPSAAAFLIARLMAK"
     gene            11352..13016
                     /locus_tag="PLP58_09055"
     CDS             11352..13016
                     /locus_tag="PLP58_09055"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HRJ08703.1"
                     /translation="MILRFLPDFSAMPRRALGVATFLLLLAVTAMEAAQQRAPNVIFI
                     LTDDQRYDELGVTGHPVMKTPHLDALARDGALFQQFFTTTAVCLPSRVNYVTGKWERS
                     HTVGFRGERALSDEQWRESLFGVLRQAGYFTGLIGKSNIPGLRQDDIDYYCGSDRTYL
                     GFYPREQHRDFEVLFHGATETTQIEIIGEAARDFLGTDDGFYQRSLPAMKKYLGRRDR
                     SKPFFLYLPFEVPHGTGTRTMAQRPTDDELYRSTYRDRIDDMPLPDNYVALSDWRTPK
                     LPLEIYNGEQSDSYSYRRTPEGVREQMVRNCQTITGIDRLVGRLRAQLEELGEAGNTI
                     ILFGSDHGWMHGEWGYGGKCLLYEPSIHVPFIVYDPRPDSIRRGTQIRELAVSPDVAP
                     TILDLCGIKPPESMQGRSLVPLMRRADAQSVEWREDIFLECLMLGQNYPLVQGVRGER
                     WKYMRYWPLRPVPDDYRDMLNFGLRGEKPAYEELYDLKNDPLENHNLAGSPPHAARLE
                     EMRARCIEALRESLGRAPTDPMPSITVEEWRKRTGDFYRMLRSAKK"
     gene            complement(13091..13960)
                     /locus_tag="PLP58_09060"
     CDS             complement(13091..13960)
                     /locus_tag="PLP58_09060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ08704.1"
                     /translation="MRATFLLATLCLTTLTARAADLQPPITVGQRVATCGHSFHVFTY
                     PQVDQIAKSAGLEHQLVGISSIGGSTVQKHWDKTEEESAVKKVLKAGGADVLTLSPIW
                     LPDPAIEEFVKLGIGHNPALRITVQEYWMPNDEYEPVYPLQTKKKVDHNATNLAKLRD
                     ATMRYAKDIEDYVKGINQRLGKDAVLIVPVGMAAVTLREKIVKGEAPGLKQQSELFRD
                     NWGHALLPLQLLSSYCHYAVIYRKSPVGLPVPLAFRTLKDVSDEDKAKLNTLLQQIAW
                     DTVSAHPMSGIKK"
     gene            complement(14080..16755)
                     /locus_tag="PLP58_09065"
     CDS             complement(14080..16755)
                     /locus_tag="PLP58_09065"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HRJ08705.1"
                     /translation="MQRDLQQLHRYHDKADASAFQSLVKLHAGMVFATARRVTGNDSL
                     AEDVAQETFLELARKGQRITSSVGAWLHRVASRRACDAVRSESARRRTEASAAILDEG
                     RECTWEEMEPVLDEALGELGEQERTAIIEHFLEARTQAEVAARLGVSQSTVSRLLDSS
                     LVRLRERLRAKGMICGAGLGAVIMQQSTLAAPHALVAGLNKIALSGIGANASSVGWLT
                     IKILLASKAKVALGITVGFLACAVGVDLASHNSWLMSLMSSDSEESMKVTGAAQKLGR
                     SDKVASSNRRQQLLAEARAIWAKAPQFSQEQWQELISKIYWSRGSEVRFMALHAIGIT
                     LSRTSYNRVMALHPNVDSFDERTALKASLVCYRALTAEWFLEAPREAVAWELALVGRR
                     AGDSSWVLSVIADWIKTHPEEWEAFVEAGNHPQLSGQAKAWTEELDNSGSIWTKITDN
                     EIKLEAVEEAIYQRTDTGDPEMILNQILRAPREKFRHENIVRLAPRLSTEQLLRAINS
                     GQFSDGSLVNMLRAMAGIEGASYAEAAKRSTDMAKRGGMEAEQANAHSACIGRIYSAW
                     LKTAPKEALRQAIQSGNSDFLPKLMTESAREGHLTEEAIVTALSSEKNRDRALASFYL
                     ASADDNPVTALQSIIGSSHVVDQVEAAKSILEAWTKRSPDEAVQWLSALPASEDRKEL
                     AAVIASQWSEIEPEAAFAFAQQYEVGLRDDWKNAFGFNARGLPEAKLAILLRAVRDDP
                     NYNIMLASLVGYRHPSQPMEAIAMLTRYGAPGWETALVDDTLRWLSGEDSRAEDYAKV
                     LPRLNLSAVEPEKIHRAAKLFIQRNANAAKISSALSWTLGFPFGISQQARSEAIQALD
                     LGDQSFRTAVQSWVAGAPLSADERKALQSIVAEKN"
     gene            complement(16837..18054)
                     /locus_tag="PLP58_09070"
     CDS             complement(16837..18054)
                     /locus_tag="PLP58_09070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009965550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HRJ08706.1"
                     /translation="MKLTAFSLLIATTSLFAADWPAWRGPTGDGHAAPGQKLPVKWSE
                     SENLVWKAAVRGRGHGSPIVVGDQVLLATADVETEEQLVLSFDRATGKSRWESVVHRG
                     NLNTKGHKISSQASSDVVSDGERLFVNFLNNGAVFTTALDLNGKQLWQRRVCDFQVHQ
                     GFGSSPVIYQNIVLVSADHRGGGKMTSLNKLTGEIVWQQDRPPVANYASPAIVTAAGK
                     VQAVLAGCNKVESFDPLTGKKLWSIDGSTEETVVTAVTDGSRVFLGGGYPKRHTMALE
                     ADGSGKIAWENVTQTYVPSMIVKSGHVFAVGDAGRAVCWHSATGEERWNEKVDREFYG
                     SPVMADSRIYVTSKSGVTSVFEATPEKFTLIGQNPLGDESFSTPAICGNRIYLRSAKT
                     SPTRQEFLWCVGE"
     gene            complement(18051..18842)
                     /locus_tag="PLP58_09075"
     CDS             complement(18051..18842)
                     /locus_tag="PLP58_09075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009962049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sterol desaturase family protein"
                     /protein_id="HRJ08707.1"
                     /translation="MLRDLFISQSWSKSTQMFLGIAIENWLRYALVAGVAWLLAYVIF
                     KNRWWKRKIIQKTPSAADVRREIKWSLLTALIYGLVGIATILAGRTWGWQMYAKISTH
                     GWGWFVASVGIAIIVHDTWFYWTHRLMHHRRLFKLFHRVHHESTNPTPWAAYSFAPLE
                     AAVQAGIFPLLVFTVPMHPLAFFAFMVWQITFNVIGHTGYEYFPKWMMRSWLGRILNT
                     PTNHVQHHEKMRGNYGLYFNYWDRLMGTNHKDYEARFAEITTRSS"
     gene            complement(18857..19903)
                     /locus_tag="PLP58_09080"
     CDS             complement(18857..19903)
                     /locus_tag="PLP58_09080"
                     /inference="COORDINATES: protein motif:HMM:NF014893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HRJ08708.1"
                     /translation="MHTPVRFALAGFGAWGKFHAQSIAGNPDAQLAAITAPSEASREE
                     ARQLYPGAEIYADSLEMIAQSDFDIIDIATPSHTHREIALAAMRKGRHVLLEKPMAIT
                     LEDCKAIVAGARQYGVNLAVGHELRLSSQWGEIKKIIERGTIGEPQYVLVELSRKPYR
                     MGASGWRYDQSRVGSWVLEEPIHFFDLARWYLESSGDPVELHAYGNSRDPRRPTLFDN
                     FSAMFKYANGSYAVVSQTLAAFEHHQTVKVSGTKGALWAAWSGALDRTLEPEYFLKVF
                     DGEKLETVKLEKHSGEVFELREEIAQCVEMVRTGKKPVATGLDGLWSAGLCLVAEESI
                     RQNKPLPVGDMLKF"
     gene            20139..20897
                     /locus_tag="PLP58_09085"
     CDS             20139..20897
                     /locus_tag="PLP58_09085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ08709.1"
                     /translation="MEAASTLFPATRWTLLRRLREGSAEESGAALEILCRAYWYPIYA
                     IGRSLGLSKADAEDATQDFFFKLLRKDYLRHADEKLGRLRSLLLKAFDNHLASEWRRE
                     KRLKRGGGLERVPWMDFLEAEERYLQTGGPCGCDMEALFAREWARSLLDRALSSLRAA
                     QEKKGNKDEFAIYEPFLVQEGAEGDTERAAVMPGKSGEAFRVTLFRLRRQFRDHVEQE
                     IALTLGSDDPSLIREEMAELMRSFSQAPTSTGKP"
     gene            20894..>22942
                     /locus_tag="PLP58_09090"
     CDS             20894..>22942
                     /locus_tag="PLP58_09090"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HRJ08710.1"
                     /translation="MKKLSPVIGHEAAILESLLGGDSWRDSAGDEIGPYTLVERLGEG
                     GFGVVWRARQTHPVRREVALKVLKPGMDTVRVLTRFERERQVLAGLGHPNIARLHEAA
                     ATADGRPCFAMELVDGEPLTEYCRRRGLALEAKLGLFLQVCAAVQHAHQKGVIHRDLK
                     PPNILVAEEGGAPVPKIIDFGISKLMNPGDLDGPELTLLTRQDALLGTPLYMSPEQLD
                     GSQDVDTRCDIYSLGVVLYELLAGEPPFDTPALLSLGTEEMHRALREDRPPRPSTRIL
                     RAGAPGSVSKRRALSLPADLDWITMRALEKDRARRYSTAAEFAADIRRFLDREPVLAR
                     PPSLAYLAGCWVKKHRALAAAASFSVLALVAGLALALWQAHEARLAQRQAETEAERSR
                     QTAAFLTSLLDEVAAEVSMGRNPEALRGGLAASQRRLAEMGDMDLRIALLDQISRLYD
                     SMSERRLTLEALSGLAELTRARHGADSPEAHAAAVRHLSLMVNHGARGEAAGLLQAMS
                     DRIEARGGRGSLEWFDVKNLLIRAWVKLPNAELAVRTAEEVLAAAGAHEIPATKHFII
                     MLTCIEAQHAAGKHGAALRQIEAAGEWIKKNGLVKVHELDLKRRKMNARRMMGEYSAA
                     AAVQREIVKEIAGRGRAHVFKEYGRLVEVECQAREHESAIAHAREALALAGEPAADGA
                     L"
BASE COUNT         4231 a         6748 c         7383 g         4580 t
ORIGIN      
        1 cttcgagcgc ccaaatcttc ttcaacgacc atggagttgg ctcctggctc atcggattga
       61 cgacttcgac gtaatcgtcg ggcttcggcg gataagtggg gcacgcgcgc tcctcccaat
      121 tcgcgccgcc atcgcgagac gcatgcaatt tagtgccgaa gtggccgtga tcgacggcgg
      181 cgtagagtgt gccatcgcgc gcatccggca gcagcattgg cacctgcacg ccgagaaagg
      241 acatgcgcgt aatttcccag ccattggtgc cgcggacggc ttcgatgagg cctttgcgag
      301 ttccgaggta gagtttggtg ctcatgatga atatgtgttt caaccgcctg acaatgcctg
      361 catgacgaaa agctccgaat caggcctcac agcgtcggtt tgcgcgcggc ggtcgcggat
      421 ggccgtgcca tcgacaaaga ccaccacatg ctgccggatg gcgttttgat catcgagcac
      481 atacccgcgc agtttcggat gcagttcaaa caccgcgtcg aggcattcgc gcacgttccg
      541 gccgtccaca cggcatttcg gcgcgtgcgt ctggttcgca aggttggcgg tgaactcgac
      601 ggtgggcacg gggtggaatg atcaggcctg cacgatggtc ttgaagccgc cgtaggccat
      661 gcgtttgcag tcgaagagca tcttgctcgg atcgcacatc ttgtggatgc gcggatcggc
      721 catgaccttc ttgttcacct ggtcgcgatg cttgcgcgat ttgtaggtga tgaaggaaaa
      781 aatgaccact tcgctgggct tggagccggc agattgggtg aaaagcgtgc cccagtcgtt
      841 tttgaggtcg tcgccgacgc actcgcggta gtcgagcgcg ccatgctctt tccagagctt
      901 gcccgcctgc cgcgcgatgc gctggtaggc ggcgaggttc ttctttggta aaggcaggag
      961 gaagccgtcg gtgtaggtgg gcatggtggt ggagtggtta gatggaagcg agatgcgcgg
     1021 cgaggcggtc gaagttttct tcgttggagg gcgcgcagat gcctttgagg gtctcactga
     1081 gctgcacggt ctcgaaagtc atgcgccagg tgaggcgtgt gctgccgttt tcgtccgcaa
     1141 aggtcatcgt catgcgaaaa tgcggctgca catgatcata aacgatgcgc tccagcggca
     1201 cgatgtcggc gaagacggtg tgattcgggt agttggcccc attggggccg tgaaatatga
     1261 acttccactc gccgccgatg cgcaagtcga aggtttcaaa ggtgctcgtg aaaccttttg
     1321 ggccaaacca gagcgtgagc tgcttcggat cgctgaaggc cgcaaacacg cgctggcgtg
     1381 aatgcggaaa cacgcgcgag ctgaggattt cgttgtcggc ggcagggttc atgggcagac
     1441 ctcggtgctg atcatccaac ccatgccgaa cttgtccttc aacatgccaa aacgcggcga
     1501 ccagaaggtc ttggcgagcg gcatggtgac ttctccacct tcggcgagag cgttgaaggc
     1561 acgatccgcc tccgcttcgg tggcgacggt gatggacagt gaaaaaccgg cgaagggcga
     1621 ggtgtcgccg caaccgtcgg aggccatgat caccgaactg ccgacgcgga agctggtgtg
     1681 catgactttg ttctcgtagc ccggcgggat catgccgggc gcgcagggct ccgggctgtc
     1741 gctgtagtgc atgagcatct cgacctccgc gccgatggcg gttttgtaga aggcgagcgc
     1801 ctcttcgcag cggccgccaa agaaaaggta gggttggatg aagggtgcgg acatggggtg
     1861 ttggggtgat gaatcagcgg cagatttcga cgagttgatc gaaggccttt gcccagcctt
     1921 cgtggaagcc catggcggcg tgcttttcgc tgttttcctt cgtccaatga agagcacggg
     1981 cggtgtagcg ggttttgccg tcggggagag tctcgaaagt gataatgccg gtgaagaagg
     2041 gattcgggtt cggctcccaa ttcgcgaaat aggcgtcggt gaagacgatt ttctcgttgg
     2101 gcacgatttc gagatacacg ccctgatttg ggaatcgctc gccatccggc ccgcacatgg
     2161 tcatgcggct gaatccgccc gcgcgcacgt cgagctcgac atcggcgaca gtccacggct
     2221 tcggacagaa ccactgcgga tacaattcgg ccgtggtcca gtcggcgtag agcttttcgc
     2281 gcggcacgtc ggtctcacgg acgagcacga gctcgcgttc ggagagcggg gattcgatgg
     2341 gagcaggcaa agacatggtt cagaggatgg ggcggttgag ttgaatcagg gcttcggcgc
     2401 ggcgacgacc ttggcgagat tcgcgaggcc ttcgttgaac tgatcgccgc acatcttctc
     2461 gcagtccatg aagaggccga ccaccttgcc gatgaagggc ttcttgctct gcatggtcca
     2521 ggtgaccttg gtgccggtgc cctcggcggc gaaggtgaac ttcacatcgg catcgccctc
     2581 gaaaggccgc acgaactgca gcttcatgcc gatctcctcg ttcggcttgc tggcgatgag
     2641 actggtcgtg ccctcaccga cctcgtcgtt gccggaccat ttgtagatcg caccgacgcc
     2701 ctcgggcggg ccttcgagcg tctttttcat gttcggatcg agcttcgacc acggggacca
     2761 cgcatcccac aggcgaaaat cgttcacgac tttgaaaaca gcctccggcg ctgccgggat
     2821 cgtggtggag cgggagacgg tcatgtcatc ggactgaaaa gacgcgacga cgaggatgat
     2881 ggcgatgacg gcgacgaggc cgagaaggat ctttttgatc atgatggtgg cgtggttggt
     2941 gtacaacaga agggatcagc tgccttgggg catggcgctc atgcgctcct gcatctgctg
     3001 cggggtgagt gtttcggtct gatgctggat catccagatg tgcccgaagg gatctgtgac
     3061 gcagccgctg cggaagccgt agaattgatc cgccaccgca cggagcaccg tggctcccgc
     3121 cgcgaccgcc tgggcgaaag cagcatccgc gttgtccacg atcagacaaa acgtgacggg
     3181 agagccgccg agtgtttgcg gcgacttgct catgccggga aactcctcgc tgagcatgat
     3241 gagcgtgtca cggatcgaca actcggcatg ggcgatgcaa tccgggccgc ccatggtgag
     3301 gcggaagagt tccttggcat cgaacgcggc tttgtagaac tcgatggcct tggcggcctc
     3361 tttgacagtg atgtaggcgc tgagcgcagg ataggtggtg ggtgtgttca tggtgattta
     3421 cttgtcaaag aggctgtcca cgaaggtgcg ggcgagcttg agattggcgc ggatcatcgc
     3481 ctgcgagtcc acggccttgc cgacgagcat ggatccctgc cagaggctgt tgagaaacca
     3541 cgcgacagcc ttcggatcga agttcgtggc cggtttgagc tgctttttcg catcggcgag
     3601 catttgggcg acattgtcgt tccagctttg cagctccttc gcacacgcgg cctgcatgac
     3661 gggatttgtc gaggccagtt cctgcgacat catgcctacc atgcacacgc agttctcgtc
     3721 cttgcgcagg gtgaagccgc tcatgatatc gagcatctga tgcagccgct ccagcggccc
     3781 gatggcggag ttctgccacg ccgcgttgta aaacgacgcg cccatctgcc cccaccactc
     3841 caccgcggcc tttccaaggg cctccttgct ctcgaaatgg tggaagaagc ctcccttcgt
     3901 catcccggcc tccttgcaga tcatgtccac cgaggtgccc gcgaagccct gcctcagcat
     3961 gagccgcacc gtggcatcaa tgagcgcctg ccgcgtcccc tcaggatcgc gctctcgctt
     4021 cttgctggcg gctttctttg gcatacaaac cggtcggtat gtaacaagac atgcagcctc
     4081 gtcaatgcgg ttttggtcaa aaatttgacc gctaccagat gcgggagcta cttcgcaccg
     4141 cctgccttct tcgctttcga cttcgcggct tcctttggat accgcacgac cttgatatcg
     4201 ttcttctgcg gtgctccagg cgtgctgcgg ccggcgcgga tgagtttttc aaaggaggcc
     4261 tgcatggctt tgacgcgttc aggatgcagc ggggccatgt tcatcgtttc ggcgaggtct
     4321 tcgtcgaggt tgtagagctc cggctcggca gcggcgacgt atttccatgc tccatctcgg
     4381 tagctcggga tgccgccgca ggcggtgttg acggcgttgg tgcggatggg gcggtcttcg
     4441 cctttcagca atggcatgag gctgaagctg tcttcacctg cggtctccgg cagtttcacg
     4501 ccgaggattt ctgccatcgt ggcgagggtg tcggcctgga gcacgagttg cttgcacacg
     4561 ctgcccgctt tgaccacgcc gggccagcgc acgacgaagg gcacgcggtg tccgccttca
     4621 tacacggagg ctttgtagtc gcgcagcgga ccgctgggat agtgcccctg cttttccatg
     4681 tccttcacgc cgatgtagga cgcgcagccg ttgtcgctgg tgaaaagcac gaaggtgtcg
     4741 ttttcgacgc cggcggtctt cagagcgtcg agcacgcgac cgagcacctg atcggtctcc
     4801 atcaccagat cggcgtaatc gctgttgagg ccgcttttgc ccttccactc ggcattcacg
     4861 gccagcggcg tgtgcggcga ggtgagcggc agatagatca aaaagggctg cttggccgct
     4921 gactgcgttt ggatgaactt ttcagctctc gacgccaacg cgggcaaaat gccctccaac
     4981 tgccagtctg ccagcgcggg accttgaatg ctggcctggt tcttcacgag ctttgacgcg
     5041 ggcagcagct cgctcgggat gccgacggtg cgatctccat caatgaagca atatggcggc
     5101 cagttgggca cgtcggtgcc gaagtattca tcgaacccac gctctgacgg cccgccgggg
     5161 atgcgctggg aaaacacagc tttccatgct tcgcgatgtg cgtcggtgat cgtcgtcgaa
     5221 acgtcgcccc cgccgccgga tttgccgccg aagccggtga ggagcacctt ttgctccggc
     5281 gtgaccggcc agtcgctgcc gagatgccat ttgccgatgc aggcggtgcg gtaaccatgc
     5341 tgctgcgcca gtttgccaat cgtcagacgg tccggcgcga tgagcggctt tccccacaag
     5401 ccgacgatgc cggattgcag tgtcgtgcgc caatgataac gccccgtgag cagcgtgtag
     5461 cgcgatggcg agcagcagcc ggaactcgaa tgcccgtccg tgaagcgcat gccctcggcg
     5521 gcaagccggt ccatgttcgg cgtcgcgatt ttgccgcgct caggatcgtt cacctgcatg
     5581 tcgccataac ccaggtcgtc cgcgaggatg atgacgaagt tcggtttcgc cgcgagcagc
     5641 ggcacgcaga ggacggagag aagcaggaag gcgagatggc gcatgagagg agctggcaga
     5701 ggcttatttg cgcgtgcgtc cggcttcgcg ggcctgggcg agctgggcgc tgagctgttt
     5761 cacgacttcc ggatgctcgg cggcgatgtc tttggtttct ccgacatctt gcgtgaggtc
     5821 gtgcagcgtc ttcttcttgc cgttcttgtt ctcagtgaac ttccagcggc ctgagcgcac
     5881 ggcgaggacg tcgtccttcg tgtcggcctg aagaatggtg atgggacgga tcggcgtggc
     5941 gagcgatgga tcgcgcaacg cgggcaggat gtcgaaggag tcctcgccgg catcgtctgg
     6001 cagctttgca cccacgatgc tggcgaaggt ggcgagcaga tcggtgaggc agatggtctg
     6061 ctccgacgtc gatgccgcag ggatctgacc gggccagcgc gcgatgaacg gcacgcgatg
     6121 cccgccctcg tagggagagc ctttgccatc gtgccagggg ccgttggtgt agtgacccat
     6181 cgcgcgggcg tcttgcttgg ccgtgtggaa tttctcgcgc acgccgccgg cctcgctggc
     6241 gaggttgaga ttcatatcat gagtgccttt gaaatcgttc ggcgtgccgc cgttgtcgct
     6301 gctgaaaatg acgagcgtgc gctccgaaag cttgagttca tccagcgtgg cgagcagatc
     6361 gcccacatgc gcgtcgagtt cctggatgta atcgccgagc aaccccgcct ggctggtgcc
     6421 gcgaaacttc gccgccggtg tgatgtgcgt gtgcgaggcg cagggcgtga agtagagaaa
     6481 gaagggctgg tctttctgct cgcggatgaa gccgacggct ttggcggtga gcgtggtgtc
     6541 cacctggtcc tccacccgcg gctgcgagac gccttcggga agatcgcgac cgtcgttcac
     6601 cacgcggtag gcctcgcccg gtttccgacc gaagatgtcg tggttctcga tgaagacacg
     6661 atactggtcg ttgtggttcc ccggcacgcc gaagtgataa ttaaacccga cttccaacgg
     6721 gccggtgatg cgcttctggt tccagtcctt gccgtaaccg agatgccatt ttccgatggc
     6781 cgccgagcgg tagccctgcg cttcgagcat ggaggcgatg ttcaggcggt cttttttgag
     6841 cttgtagccg tttcccttcc gtcccagccg ccacggcgag agtcccgtca tcactccata
     6901 gcgggatggc gagcacaccg acgcgggcga atgcgcgtcc gtgaagcgcg cgccctcccg
     6961 cgccagccgg tcaatgttcg gcgtctggat cttcgtcgcg ccctggcaac tgatgtctcc
     7021 ccagccgagg tcgtcggcga ggatgacgat gaagttgggc ttcgtgtccg cgaagagcgg
     7081 cgaaaccaag agggcaaacg acgacaggaa tgagagaagg cgtgaagaag agaagaagtg
     7141 tttcatggtt tggtgattgg gctgagtgaa ctgaatgcgc caggtttcac ggaggagtgc
     7201 gtcactcttt gtcaatgatg cggtccactt tggctccgcg attctgctca attgtgacgg
     7261 cgtcctggtc gggaccctcg acgcggatgc tccggttgtc gccaatcagg aagttgttct
     7321 ggatgaggat gtcgcgaacc tcctcgcgag tgtccacgcg gatggcggcg agctggtggg
     7381 catcggtctc gatgatgttg tcggcgatga ggtaggaggc ggtgggcgtc gcgaacttgt
     7441 cgccgcgggc ctcgttgatg gtgatgttgg gattccagag atgatggcgc tccgtgccgt
     7501 tgttcttgcg gccgttcagc gagaagacgt tgccggtcac gacgcaacgg gccgaatcgg
     7561 gtatccaaag gccctcgcgg ccgctgcctt cgatggtgtt tccgatgagc gtgcagaagt
     7621 cgcttttgcg ctcgatggtg acgccgcgcg agccattgtc gcgcatgacg ttgttggccg
     7681 cgagcacatg ctggcaggtc tcaaggaagt agccgcccat cttggcgcgg tagattcgat
     7741 tgtcggtgaa ctgtccgcgc cggcaatagt agaggtgcgt gccgtccgag cgcgagtcct
     7801 cgaaccggca tccgcgcacg acgaaatcgt ggacatggta gagagcgatg aggccctggt
     7861 cctcgcgggt ggagcctttc tcagggccgc gcttggactc ggcctggtag tcaccataga
     7921 cgttggcgca gttatcaatg acggtgtcgg tgatccagac atcggtggac ggatgttcct
     7981 cgtcggcagc aaagatgccg atgccattgc tggagaggtc gcggatgtgc atgtcgcgga
     8041 tgcggatgtc gcggcactct ccggtgagat ggacgccgcg gatgttgacg ccggtcagcc
     8101 agacgtcgtt gcgaccgatg atggtcccgc cctcaaggcg cagaccggag actccctggc
     8161 cgaggaaaag ctggccgtcg gcggcgcagg tttctgaaag caggaacgtg gcgccctcca
     8221 tgctcagggt caggttggag cggagacgaa gtccgaccgg gtcgtcgatt cgataagtga
     8281 tgggggcgaa gacaaccgtg ccgccggtgc cgggaacggc gtcaatggcg gactgaattg
     8341 cagattggta ggaaacggag ccgtcagtga cgtgatcggc tggaaggaaa tcaacgacct
     8401 ggatgacggc cgtggcggtt gggggcttgg cggcggaagc cgtcaacggt gcaaagagac
     8461 tcgtgacagc gaccaggagg agcatggttt gtttcatggt ttggcatggt tgattccaga
     8521 ttttgagtgg gccggggggg ttgcgccatt gattctaccc gggaactgcc caatctgtca
     8581 cgacaaagac tatgcgaaac gcgacacccc tccagccccg gtcccggcag tcgcggcgtt
     8641 ccctggcgga aatccagcgc tggcgcgcgc gccgcgccgc ctggttctcg cagctcgatg
     8701 ccgcgtgtgg tttccaagca ctcttcgacc acatccccgc ggtgttcttc ttcgccaagg
     8761 atcgcgaagg ccacctgatg tttgccagca agggccttct cggccgctat cagatgcgcg
     8821 acgacagcga gttcatcggg cgcacggatt tcgacctcaa tcccggcagc atggcacagg
     8881 cgtatgtgga agatgacgcg aagcttctgc ggggccgggc gccggccatc gagcgcatgg
     8941 agctgtggtg ggaccggcag ggcatgcccg actggtatct cgtgaccaag ctgccgctgc
     9001 gcgaccggcg cgggcgcgtg caaggcatca tgggcatcct gcgccgcgcc gatgagcacg
     9061 accgcaggct ccccgtcttc cagactgtct cgcgcgcggt ggagatcatc cgccgtgact
     9121 tcgcccggcc catcaccatc ggcgaggtgg ccggaatctg cggcgaatct ctgcgccagc
     9181 ttcagcggcg ctttctcatc gccttcggga tcgggccgca ggagtttctc atcaagacgc
     9241 gtgtcctcgc ggcggcccgc ctgctcgacg agagtgatct caccgccgcg gaagtcgcca
     9301 cgcgctgcgg cttcgtggac gcgagcagct ttacccagca gttcaagcaa cgcaccggca
     9361 ccacgcccgc gcggtatggg aagcgccgaa cccccaccgg ccccgggcct tcggcaggaa
     9421 gggagccgag gggttgaaca aggaaattcc tgccagccag agagctgcga gatgcttttt
     9481 cccggatcgt tcttccagct tgccatgaag cacctcgccc tgatcctcct cgcactctgc
     9541 cctctgcttt ccgccttcgc ggcggacaag cccgcgcatc tcttcatcct caccgggcag
     9601 tcgaacatgg cgggcatgga tccgaagctc ggcttcgagc cggaggcgaa gaagctgttt
     9661 cccggcgcgg acgtggccca catcaaggtc gcggtcggcg gcatgcccat tcgctactgg
     9721 gtggacgagt ggaacgacat cgccgcgaag cacggcgtcg atgtcgcgaa ggcgcgcgag
     9781 gcggacaagc gcaagggcac catcttctac ccgcgcatcc tggagcagtt cgcggagctg
     9841 ctgaaacagc acccgaatgc agcctcggtc accttttgct ggatgcaggg cgagaatgac
     9901 tcgagaaccg ggctgcaagc gccttatgcc gacgctttga agcaactcat cgccaacctg
     9961 cgccgcgacc tgaagcgccc ggagatgaac gtcgtcatcg gccgcctcag cgaccacgac
    10021 gagctcaacc cgtccgcgtg gaaagtcgtg cgtgagagcc aggttgccgt cgctctggcc
    10081 gacccgcatg gcgcatgggt cgattgcgat gatctgaacg acaaggaagt caaaggcgtg
    10141 atgaccaacg acctccacta caccaagccc ggctatgagc tgctgggccg gcgcttcgtg
    10201 cgtcaggcga aggcactgat cgaaggcgga aaacctgcgg agaatggccg gccggagtga
    10261 ttcaacccat tttctgatac atcagccgtt tggacgtgtt attcgcgcct gccatgatca
    10321 ctcaccgctt tcccctcgtg cttggcctcg tgtttctcgt gtcgctcccc gccgcggcgc
    10381 agcagaaggc caggaaggag actgcgaaag gtccgccgcc cactttcgcc gatgtgcctt
    10441 atggcacgga cgaacggcag gtgctcgatt tttatcaggc caagtccggg cagcccgcgc
    10501 cgctcgttct tttcattcac ggcggcggct ggcgcggcgg ggacaaggac cgcgctgccg
    10561 gttatggtct caatgagtat ctcgaagccg gcctgtcggt ggcggcgatc aattaccgct
    10621 ttgtccccgc cgcgcatgag gcgggcatca agccgccggt gaagtggccg ctcgaggacg
    10681 cggcgcgggc gctgcaattc gtgcgcagca aggccgggga gtggcgcatc gacaagacac
    10741 gcatcggtgc cacgggaagc tcggccggcg ccgcctccag cctgtggttg ctgtttcatg
    10801 atgacatggc ggatgccggc gcgagtgatc cggtcgcccg cgaatccaca cgcctgacct
    10861 gcgccggggt gctccacgcc cagacctgct tcgaccctca tctgctgcgc gcctggatgc
    10921 ccaacatgac ttatggcggc cacgccttcg gcttcatcaa agacgggcag acacgcaagg
    10981 atgagttcca ggcgttccat gacgggcgcg agcaaatcat gccctgggtg aaacaatact
    11041 cgccctatca tttggtgacc gccgatgatc cgcccgtgct cttgcaatac gacgcccgag
    11101 ccgatgaacc gcccgtggtg gggaagcggg ccggcgatgc gccgcattcc gcggttatgg
    11161 gcatcaagct gctggaaaag ctgcgcgaga ccggggtgga gggccacctt gtttatcctg
    11221 gcggaccaga aagctcctac ccgtcggcgg ctgccttctt gattgcgcgc ttaatggcaa
    11281 aatgagggtg tcacggcagg ccgaaacgcc gcgttgatcc tgccggcacc gtcaaatctt
    11341 cgaccctttt tatgatcctc cgattcctgc cagatttttc cgcgatgccc cgccgtgcgc
    11401 tcggtgtggc cacctttctt ttgctcctcg ccgtgacggc gatggaggcg gcgcagcagc
    11461 gggcacccaa cgtcattttc atcctgaccg acgaccagcg atacgacgag ctgggggtga
    11521 ccggacatcc ggtgatgaag acgccccacc tcgacgcgct cgcccgcgac ggagcgttgt
    11581 tccagcagtt cttcacgacg acggcggtct gcctgccgtc ccgcgtcaac tatgtgacgg
    11641 gcaagtggga acgaagccac accgtgggct ttcgcgggga gcgcgcgctt tcggacgagc
    11701 agtggcggga gtcgcttttc ggggtgctgc ggcaggccgg ctacttcacc ggtctgatcg
    11761 gaaagtccaa catcccgggg ctgcggcagg acgacatcga ctattattgc gggagcgaca
    11821 ggacctatct cggcttctac ccgcgcgagc agcatcggga cttcgaggtg ctcttccacg
    11881 gtgccaccga gaccacgcag atcgagatca tcggcgaggc ggcgcgcgac ttcctgggga
    11941 ccgacgacgg cttctaccag cgttcgctgc cggcgatgaa gaaatacctc ggccggcgtg
    12001 atcgctcgaa gccgttcttt ctctatctgc cgttcgaagt gccccacggc acgggcacgc
    12061 gcacgatggc gcagcggccg accgacgacg aactctatcg ctcgacctat cgcgaccgga
    12121 tcgacgacat gccgttgccg gacaattatg tggccttgag cgactggcgc acgccgaagc
    12181 tgccgctcga gatctacaac ggcgaacaga gcgacagcta cagctaccgc cgcaccccgg
    12241 aaggcgtgcg cgaacagatg gtgcgcaatt gccagacgat caccggcatc gaccggctgg
    12301 tgggccggct gcgcgcacaa ctcgaggagc tgggcgaggc cggcaacacc atcatcctct
    12361 tcggctcgga tcacggctgg atgcacggcg agtggggcta tggcgggaag tgcctgctct
    12421 acgagccgtc gatccatgtg ccgttcattg tctatgatcc gcggccggac tcgatccggc
    12481 gcggaacgca gatccgggag ctggcggtgt cgcccgatgt tgcgcccacg atcctcgacc
    12541 tctgcggcat caagccgccg gagtccatgc agggtcgcag cctcgtacca ttgatgcgcc
    12601 gggctgacgc gcagtccgtg gagtggcggg aggacatctt cctggagtgc ctgatgctgg
    12661 ggcagaatta tcccctcgtg cagggcgtgc gcggcgagcg ttggaaatac atgcgctact
    12721 ggccgctgcg cccggtcccc gacgactacc gcgacatgct gaatttcggc ctgcgcgggg
    12781 aaaaaccggc ctacgaggag ctctacgatc tgaagaacga tccgctggag aaccacaacc
    12841 tcgcgggcag cccgccgcac gcggccaggc tggaggaaat gcgtgcgcgg tgcatcgaag
    12901 ccctgcgcga gtcgctgggg cgcgccccaa ccgatccgat gccgtcgatc acagtcgagg
    12961 aatggcgaaa acgaacgggc gacttttacc gcatgcttcg cagcgcgaag aaatagcctg
    13021 ggccatcatt ctccaatgat ctcatgtgcc agcggtcgcg ggcgaatgcg cgtccgtgaa
    13081 gcgcacgcgg tcattttttg atcccgctca tgggatgcgc gctcaccgtg tcccaggcga
    13141 tctgctggag cagcgtgttg agcttggctt tgtcctcgtc gctcacgtct ttgagcgtgc
    13201 gaaatgccag cggcacgggg agaccaacgg ggctcttgcg gtagatcacc gcgtagtggc
    13261 agtaactgga gagaagctgg agcggcagca gcgcatggcc ccaattgtcg cggaagagct
    13321 cgctttgctg cttcaggccg ggcgcttcgc ccttcacgat cttctcacgc agcgtcaccg
    13381 ccgccatgcc gaccggcacg atgagcaccg cgtccttccc gaggcgctga ttgatgcctt
    13441 tgacgtagtc ctcgatgtcc ttcgcgtagc gcatcgtcgc atcgcgcagt ttggcgaggt
    13501 tcgtggcgtt gtgatcgacc ttcttcttcg tttggagcgg atagaccggc tcatactcgt
    13561 cattcggcat ccagtattcc tgcacggtga tgcgcagcgc gggattgtgc ccgatgccga
    13621 gcttcacaaa ctcctcgatg gccggatcag gcagccagat cggcgaaagt gtgagcacat
    13681 ccgctcctcc agctttcagc accttcttta ccgcgctctc ttcctcggtc ttgtcccaat
    13741 gcttctgcac cgtggagccg ccgatgctcg aaataccgac gagctgatgc tccagccccg
    13801 ccgatttggc gatctgatcg acctgcggat aggtgaacac gtgaaaactg tgtccgcagg
    13861 tggcaacgcg ttgcccaacc gtgattggcg gctgcaaatc ggctgcacgg gcggtgagtg
    13921 tggtgaggca gagggtggcg aggagaaagg tagctctcat ggcctcaaga acgtcgagga
    13981 aggctcgaat cgctcattgg gatgcatgaa caggtgctgg cagaaagatg ggggaagcaa
    14041 atagcagagg ccattttctg cccccccatt tgctgtcagc tagttcttct cagccacgat
    14101 actttgcaat gccttgcgct catctgccga cagcggcgcg ccggcaaccc atgactgcac
    14161 ggctgttcga aaggattgat caccgagatc gagggcttgg atcgcttctg atcttgcctg
    14221 ctgcgaaatg ccaaaaggga agcccagtgt ccagctaagg gccgagctga tcttcgcggc
    14281 atttgcattt cgctgaatga acaacttcgc tgcacgatgg atcttttctg gctccacggc
    14341 actcaagttc aacctcggca gcaccttggc ataatcttct gcacgactgt cttcaccact
    14401 cagccaacgg agagtgtcat caacgagggc tgtttcccaa cccggcgcac cgtaacgggt
    14461 aagcatggcg attgcctcca taggctgact ggggtgacga taacccacga ggctggctaa
    14521 catgatgttg tagttgggat catcacgcac cgcccgcagc agaatggcaa gcttggcctc
    14581 tggcaggcct cgcgcgttaa acccaaaggc gtttttccaa tcgtcgcgca ggccgacttc
    14641 gtattgctga gcgaaggcaa aggcagcctc tggctcgatc tctgaccatt gtgaggcgat
    14701 cactgctgcg agttccttgc gatcttcact ggctggtagt gctgagagcc attgtacagc
    14761 ttcatcaggc gaacgtttag tccatgcctc aaggatcgac ttggctgcct ccacttgatc
    14821 aacgacatgg ctggaaccga tgatgctttg tagcgcagtt actggattgt catcggcact
    14881 tgcgaggtaa aatgaagcta gggcgcggtc acgattcttt tcactggaga gcgctgtcac
    14941 gatcgcctct tcggtcagat gtccttctcg tgcagactca gtcatgagct tgggcaggaa
    15001 gtcagagttc cctgactgaa tggcttgtcg cagagcttct tttggcgcgg tcttcagcca
    15061 ggcagagtag atccgcccga tacacgcact gtgggcgttg gcttgctcgg cttccattcc
    15121 accacgcttg gccatgtcag ttgaccgttt ggcggcttca gcataggagg ctccttcgat
    15181 acctgccatg gcgcggagca tgttgaccag actgccatcg gagaactgcc ccgaatttat
    15241 ggcacggagc agttgttcgg tactcagcct gggagcaaga cgaacaatgt tctcatggcg
    15301 aaatttctct ctcggagcgc gcagaatctg attcagtatc atctcgggat cgcctgtgtc
    15361 tgtgcgctgg tagatagcct cttccactgc ttccagcttg atttcattgt cagtgatttt
    15421 ggtccagatg gagccgctgt tgtccagttc ttcggtccac gccttagctt gtccactgag
    15481 ttgtgggtgg ttccctgctt cgacaaatgc ctcccattct tcgggatgag tcttgatcca
    15541 atcggcgatg accgagagca cccaactgct gtccccagct ctacggccta ctagcgcgag
    15601 ttcccaagcc acagcctcgc gtggtgcctc caaaaaccac tcggcagtca atgcgcgata
    15661 acaaactagc gaagctttta atgcagtgcg ctcgtcaaat gaatcgacgt tcggatgcaa
    15721 tgccatcact cgattgtacg aggtgcgtga aagggtgatg ccgatagcat gaagtgccat
    15781 gaatcgcacc tcagaacccc gagaccaata aatctttgat attaattcct gccattgttc
    15841 ctgagaaaat tggggggcct ttgcccagat cgcccttgct tcggctaaca actgctgtcg
    15901 tcgattgctt gaggcgactt tgtcagacct gccaagcttt tgagcggcgc cagtgacctt
    15961 catgctttct tcggaatcac tgctcatcaa tgacataagc cacgagttgt gggatgcgag
    16021 atcaacaccc accgcacagg cgagaaatcc cacagtgatg ccaagagcca cttttgcttt
    16081 tgacgcgagc aggatcttga tcgttagcca tccaacgctt gatgcattgg ctccgattcc
    16141 gctgagggcg atcttgttga gtccggcgac caaggcatga ggtgcggcca gagttgattg
    16201 ctgcatgatg actgcaccca gcccggcacc acaaatcatg cctttggcac gcagtctctc
    16261 acgcagccgg acaagactgc tatccagcaa ccgcgacacg gttgattgac tgacacccag
    16321 ccgcgccgcc acctctgcct gtgtgcgggc ctccagaaaa tgctcgatga tggctgttcg
    16381 ttcctgctct ccgagttcac cgagcgcctc gtcaagcacg ggttccattt cttcccaggt
    16441 gcattcgcgc ccctcatcga gaatggcagc gctggcctcg gtcctgcgtc tagcactctc
    16501 tgaacgaacc gcatcacatg ctctacgaga cgcgacgcga tgcagccatg cgcccacaga
    16561 cgaggtgatc cgctgcccct tgcgtgccag ttccaaaaat gtctcctggg ccacatcttc
    16621 agcaagcgag tcattgcccg tcactcgtct ggccgtggca aagaccatcc cagcgtgaag
    16681 ctttacaagc gattggaacg ctgaagcatc ggctttgtcg tggtaacgat gaagttgttg
    16741 gaggtctctt tgcatgattt tccgggagtc ggatgagcca tgggtgatta tgcaataaat
    16801 cctccatggc tgacagaaaa tcagaggcag caaacgtcac tcacccacgc accacaaaaa
    16861 ctcctgccgc gtggggctgg tcttggcgga gcggaggtag atgcggttgc cgcagatggc
    16921 gggcgtgctg aaggattcgt cgccgagcgg gttttggccg atgagggtga atttttcggg
    16981 cgtggcctcg aagacggaag tgacgccgct tttgctcgtg acgtagatgc gcgagtcggc
    17041 catgaccggc gagccgtaga attcgcgatc gactttttca ttccagcgct cctcgccggt
    17101 cgcagagtgc cagcacacgg cacgaccggc atcgcctacg gcgaagacgt ggccgctttt
    17161 cacgatcatg gacggcacat aggtctgcgt gacgttttcc cacgcgatct tgccgctgcc
    17221 atcggcttcg agggccatgg tgtggcgttt ggggtaaccg ccgccgagga agacgcggct
    17281 gccatcggtc acggcagtga cgacggtctc ctccgtgctg ccgtcgatgc tccagagctt
    17341 tttgccggtg agcggatcga agctctcgac tttgttgcag ccggcgagca cggcctggac
    17401 cttgcctgcg gccgtcacga tggcgggcga ggcgtagttg gcgacgggcg ggcgatcctg
    17461 ctgccacacg atctcgccgg tgagtttgtt gaggctggtc attttgccgc cgccgcgatg
    17521 atcggcggag acgagcacga tgttttgata aatgacgggc gaggagccaa agccctgatg
    17581 cacctggaaa tcgcacacgc gacgctgcca gagctgtttg ccgttcaaat cgagagcggt
    17641 ggtgaaaacg gctccgttgt tcaaaaagtt cacgaacagc cgttcgccat cgctcacgac
    17701 atcggaggaa gcttgcgacg aaattttgtg gccctttgtg ttgaggtttc cgcgatgcac
    17761 gacgctctcc cagcgcgatt tgcccgtcgc acgatcaaag ctgagcacaa gctgctcctc
    17821 tgtctccaca tcggccgtcg cgagcagcac ctgatcaccc acgacaatcg gcgaaccatg
    17881 cccacgacct cgcacggcgg ctttccagac gagattttcg ctctcgctcc atttcactgg
    17941 cagtttttgg ccaggagccg catggccgtc gcctgtgggc ccgcgccacg caggccagtc
    18001 agcggcgaaa agagatgtgg tggcgatgag gagcgagaag gcagtgagtt tcatgaagag
    18061 cgtgtggtga tttcggcaaa gcgggcttcg tagtccttgt gattggtgcc catgaggcgg
    18121 tcccagtaat tgaagtaaag gccgtagttc ccgcgcatct tctcatgatg ctgcacatgg
    18181 ttggtgggtg tgttgaggat cctgcccagc cacgagcgca tcatccattt ggggaagtat
    18241 tcatagccgg tgtggccgat gacgttgaag gtgatctgcc agaccatgaa ggcaaagaag
    18301 gccagcggat gcatgggcac ggtgaagacg agcagcggga agatgccggc ctgcacggcg
    18361 gcctccagcg gcgcaaaaga gtaggcggcc cagggcgtgg gattcgtgct ctcgtgatgc
    18421 acgcggtgga agagtttaaa taaacgccgg tgatgcatca gccggtgcgt ccaataaaac
    18481 caggtgtcat gcacgatgat ggcgatgccg acgctggcca cgaaccagcc ccagccatgc
    18541 gtgctgatct ttgcatacat ctgccagccc catgtcctgc cggccaggat ggtggcaatc
    18601 cccacgagac cgtaaatgag cgcggtgagc agggaccact tgatctcccg ccgcacatcg
    18661 gcggcggacg gtgtcttttg aatgatcttc cgtttccacc agcgattctt gaagatcaca
    18721 taggccagca gccaggcgac gccggccacg agcgcataac gcagccagtt ctcgatcgcg
    18781 atgccgagaa acatctgcgt ggacttggac cagctttgcg aaatgaagag atcacggagc
    18841 atggcagcag cgaacctcag aatttcagca tgtcgccaac cggcagcggc ttgttctggc
    18901 ggatggattc ctcggcgacg aggcagaggc cggcactcca gaggccatcg agacccgtgg
    18961 cgacaggctt tttgcccgtg cgaaccattt ccacgcattg ggcgatttcc tcgcgcaact
    19021 cgaagacttc gccgctgtgt ttttcgagct tcactgtttc cagcttctct ccgtcgaaaa
    19081 ccttgaggaa gtattcaggc tccagcgtgc gatccaaagc accgctccag gcggcccaaa
    19141 gagcaccctt ggtgccgctg accttcaccg tttgatgatg ctcaaaggcg gcgagggtct
    19201 gagagacgac ggcgtagctg ccgttggcgt atttaaacat ggcgctgaag ttgtcgaaca
    19261 aggtcggacg ccgcggatcg cgtgagttgc cataggcgtg gagttccacg ggatcgccgc
    19321 tgctctcgag gtaccagcgc gcgaggtcga agaagtggat cggctcctcc agcacccagc
    19381 tcccgacgcg gctctgatcg tagcgccagc cagaggcgcc catgcggtaa ggtttgcgcg
    19441 aaagctccac cagcacgtat tgcggttctc cgatggtgcc gcgctcgatg atcttcttga
    19501 tctcgcccca ctgcgaggag aggcgcaatt cgtgcccgac agcgagattc acgccgtatt
    19561 ggcgcgctcc ggcgacgatg gccttgcaat cttcgagcgt gatggccatc ggcttttcca
    19621 ggagcacatg ccgacccttc cgcatagcgg cgagcgcgat ctcgcgatgt gtgtggctgg
    19681 gtgtggcgat gtcaatgatg tcaaagtcgc tctgcgcgat catctccagg ctgtcagcat
    19741 agatttccgc cccggggtag agctggcggg cttcctcgcg cgacgcctcg gagggcgcgg
    19801 tgatggcggc gagttgagcg tccgggtttc cggcgatgga ttgggcgtgg aattttcccc
    19861 aggcgccgaa tccggcgaga gcgaagcgaa caggtgtgtg catgtctgaa aaggaatcca
    19921 gagtaacagg ccaaagccgc cggtatttca atcattgaac gagcgcgacg gggttgctgg
    19981 gggatgcgtt ttcaaggcta cggcactccg caaccccggc atcatggatt catcgtttgt
    20041 tgtgacgcgg ggcatgcggc gcccgccatg cggcctgccc aaaatacccg ccgtcaatga
    20101 ttgaatgaag gccgcaaggc tgtttactgg cgctgaaaat ggaagccgcc agcactcttt
    20161 tccccgccac acgctggacg ctgctgcgcc gtctgcgcga aggcagcgcc gaggagtccg
    20221 gcgccgcgct ggagatcctg tgccgcgcat actggtatcc catctatgcg attgggagga
    20281 gcctcggttt gagcaaggcg gacgcggagg acgccacaca ggactttttt ttcaagctgc
    20341 tgcgcaagga ctacctccgc catgccgacg agaagctcgg aaggctgcgc tcgctgctgc
    20401 tgaaggcttt tgacaatcac cttgccagtg agtggaggcg cgagaagcgg ctgaagcgcg
    20461 gcggcggcct tgagcgagtg ccgtggatgg actttctgga agccgaggag agatacctgc
    20521 aaacgggcgg accgtgcggc tgcgacatgg aggctctttt cgcgagggaa tgggcgcgct
    20581 cgctgctgga ccgcgcgttg agcagtctgc gcgccgcgca ggagaagaag ggcaacaagg
    20641 atgaattcgc catctacgag ccttttctgg ttcaggaggg ggcggagggt gacaccgagc
    20701 gggcggccgt gatgccgggg aaatccgggg aggcgtttcg cgtgaccttg tttcgcctgc
    20761 gacggcagtt ccgtgaccat gtggagcagg agatcgcgct cacactgggc agtgacgatc
    20821 cctcgttgat ccgggaggag atggccgaac tgatgcgctc cttctcacaa gcccccacca
    20881 gcaccggcaa gccatgaaaa agctcagtcc ggtcatcggc catgaggcgg cgattctgga
    20941 gagcctgctc ggcggggatt cgtggcggga ctcggcggga gacgagatcg gtccctacac
    21001 cctggtggag cggctgggcg agggaggctt cggcgtggtg tggagggcgc gccagacaca
    21061 tcccgtgcgc agggaggtgg ctttgaaagt gctcaaaccg gggatggaca ccgtgcgcgt
    21121 gctgacccgc ttcgagcgcg agcgccaggt gctggccgga ttggggcatc caaacatcgc
    21181 caggttgcat gaggccgcgg caacggcgga cgggcggccc tgctttgcga tggagctggt
    21241 ggacggcgag cctctgaccg agtattgccg caggcgcggt ctggccctgg aagcgaaact
    21301 ggggctgttt ctccaggtct gcgccgccgt gcagcacgcg catcagaagg gtgtcatcca
    21361 ccgtgatctc aagccgccga acatccttgt ggccgaagag ggaggagcac cggttccgaa
    21421 gatcatagat ttcggcatct caaagctcat gaaccccggc gatctcgacg ggcccgagct
    21481 gacgctgctc acccgccagg acgcgctgct gggcacgccc ttgtacatgt ctcccgagca
    21541 gctcgacggc tcgcaggatg tggacacccg gtgcgacatc tattccctcg gcgtggtgct
    21601 ctatgaactg ctggccggcg agccgccctt cgacacgccg gctctgctct ccctgggcac
    21661 ggaggaaatg caccgcgccc tgcgcgagga ccgtccgccg cggccctcga cccgcatctt
    21721 gcgcgcgggc gcgccgggtt ctgtttcgaa gcggcgcgct ctctctctgc ccgccgacct
    21781 ggactggatc acgatgcgcg ccctggagaa ggaccgcgcg cggcgttact cgacggcggc
    21841 ggaattcgcc gccgacatcc gtcgtttcct ggaccgggag cctgtgctgg ccaggccgcc
    21901 cagcctggcc tatctggcgg gctgctgggt gaaaaagcac cgcgccctgg ccgccgccgc
    21961 cagcttcagc gtcctcgcgc tggtcgcggg cctggcgctg gcgctctggc aggcgcatga
    22021 ggcgcggctt gcccagcggc aggcggagac ggaggcggag cgcagccggc agacggcggc
    22081 ctttctcacc tcactgctgg atgaagtggc ggccgaggtg agcatgggga ggaacccgga
    22141 ggctttgcgc ggcggccttg cggcgagcca gcggcggctt gccgaaatgg gtgacatgga
    22201 tttgcgaatc gctctgctgg accagatcag caggctgtat gacagcatga gcgagcgcag
    22261 actcaccctt gaggcgttgt ccggattggc ggagctgacc cgcgcgcgtc atggcgcgga
    22321 cagcccggag gcgcacgcgg cggcggtcag gcacctttcc ctcatggtca accacggagc
    22381 gcgcggcgag gcggccggcc tgctccaggc catgagcgac cgcatcgagg cgcgcggcgg
    22441 acgcggcagc ctcgaatggt tcgatgtgaa gaacctgctc atccgggcct gggtgaaact
    22501 gccgaatgcc gaactggcgg ttcgcaccgc ggaggaggtc ctggcggcgg cgggcgcgca
    22561 tgagattcca gccacgaagc acttcatcat catgctcaca tgcattgagg cccagcacgc
    22621 ggcgggaaaa cacggggctg ccttgaggca gatcgaagcc gccggtgaat ggatcaaaaa
    22681 aaacggcctg gtgaaagtcc atgagcttga tctcaagcgc cgcaagatga acgcccgccg
    22741 catgatgggc gaatactccg cggctgccgc cgtgcagcgg gagatcgtca aagaaatcgc
    22801 gggccgcgga cgcgctcatg tttttaaaga gtatggcagg ctggtcgaag tggagtgcca
    22861 ggcccgggag catgagtccg ccatcgccca cgcgcgggag gccctggccc ttgcgggcga
    22921 acccgccgcc gacggcgcgc tc
//
LOCUS       DAOMYJ010000067        22925 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Prosthecobacter sp. isolate AMR_MDS_5629
            NODE_809_length_22925_cov_22.094447, whole genome shotgun sequence.
ACCESSION   DAOMYJ010000067 DAOMYJ010000000
VERSION     DAOMYJ010000067.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867850
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prosthecobacter sp. (wastewater metagenome)
  ORGANISM  Prosthecobacter sp.
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Verrucomicrobiaceae; Prosthecobacter.
REFERENCE   1  (bases 1 to 22925)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 22925)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.30
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:34:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,742
            CDSs (total)                      :: 4,684
            Genes (coding)                    :: 4,676
            CDSs (with protein)               :: 4,676
            Genes (RNA)                       :: 58
            tRNAs                             :: 55
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22925
                     /organism="Prosthecobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_809_length_22925_cov_22.094447"
                     /isolate="AMR_MDS_5629"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1965333"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..775)
                     /locus_tag="PLP58_09095"
     CDS             complement(<1..775)
                     /locus_tag="PLP58_09095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ08711.1"
                     /translation="MGRTRKQTRDLLLPGATGWERWTGAPGERCVKAAEFGQSAGVFA
                     RDSQDRALALPAAHLWVLPAWMEGESAHLKDMAALHLERLGVRVADMEHGLHVREIMA
                     REGGILTRITALKETGAPLWDASRLPHEVLPHFACLPLPEDGIVLLRELGRLVILVTH
                     GAEVIYASPLSARELDARALGEVNLICVQLGFQGVLGRVREIQLWLEDGNLDEVTRVT
                     GLPAARMERPAPVMPNHGVSPLMPPEILHARAAARSRSRA"
     gene            complement(759..1694)
                     /locus_tag="PLP58_09100"
     CDS             complement(759..1694)
                     /locus_tag="PLP58_09100"
                     /inference="COORDINATES: protein motif:HMM:NF015867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspK"
                     /protein_id="HRJ08712.1"
                     /translation="MRLPRQRPARHQGSALIAVFWMIAVMGMLVYAGARALDTDARHT
                     RLMRGRVYAKRLAQMGLEIGRHPALPAHDPLLSYQTPDGGSYQVRIVAEEARLNINVL
                     LGSDDRVLLPRLFASWGLKAEDASALHDALHDWVDADDRTRLNGAEKRDYEKAGLIGL
                     PFNRPFKEVEEMRLVRGMDIVDALRPDWREWFTVLGDGRVDVNDARPELISLLANVPM
                     ERLAPLLTLRAGRDGVRDTQDDVRLQSAVQVAQLLGIFQPQIVEHLTRWTQFAGPIRR
                     IESTGTFGDVRRRLVLIVQDQQAIWRGEVPHGQDS"
     gene            complement(1698..2567)
                     /locus_tag="PLP58_09105"
     CDS             complement(1698..2567)
                     /locus_tag="PLP58_09105"
                     /inference="COORDINATES: protein motif:HMM:TIGR02532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HRJ08713.1"
                     /translation="MFAPQGFTLLEVIMALGLITLILGGVYGIADGSLRLGASMSRAR
                     LMETRVSHFTMTWRDYLENLPPGIRLSAGLEKGARGSAGALLIEAGPAPFAWSQALAL
                     ADAVEFAVVRGDREKSLTLLVRHLKRLEKPTPLENYQLMAELPLLQNLKTFRASYYDA
                     GEKKWFASWDPDKRPAPPLFMRLNFAFLDDPREHELVFWIANDLKTVMNVNSSQEGLG
                     NHEWTRMNTNQKRQRAALAGYGMPTLSLARTLHTTEALPRPHTIRVHSCPFVVPLLLQ
                     ISNFELRTSNLPV"
     gene            complement(2629..3081)
                     /locus_tag="PLP58_09110"
     CDS             complement(2629..3081)
                     /locus_tag="PLP58_09110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ08714.1"
                     /translation="MDSAAEPTPVSPKGFTPTASLQHSHGFALLEIILALGLFSLVAV
                     GLTRALDMIAQTSRQARQEAQVLRVLESVLAEVSHQPELKPTTVTFPKSADHVEARAT
                     ISKAELITKDKTQLDHIFHIQAEAWIEDGRKKVLKRQMQTYVYSPASP"
     gene            complement(3231..3644)
                     /locus_tag="PLP58_09115"
     CDS             complement(3231..3644)
                     /locus_tag="PLP58_09115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ08715.1"
                     /translation="MKPAFTLLEVCLALTLATLIFGVAVLGISGVQAEAGLKRSASQI
                     ETTARESLLRAVTSQRTIVLGLPGGLGIEGQMLIKRHGEKEFRAPKRGEVWEFNPTGI
                     CEPLEVRLSSPAGIIELGFDPLTACAIKKNVIVNG"
     gene            complement(3756..4190)
                     /gene="gspG"
                     /locus_tag="PLP58_09120"
     CDS             complement(3756..4190)
                     /gene="gspG"
                     /locus_tag="PLP58_09120"
                     /inference="COORDINATES: protein motif:HMM:TIGR01710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system major pseudopilin GspG"
                     /protein_id="HRJ08716.1"
                     /translation="MKTHRIHPRRTAAFTLVEMVLVLGIVALLVGAGIVSLVGVLDSG
                     KKTRVKGDLNTLSAALRSYETDNMFLPSSEQGIMALVQKPSSRPAPQNWAPKLKKVLL
                     DPWGNPYHYRRPGQNDKGGFDVFSAGEDGTPGTADDIGNWDL"
     gene            complement(4293..5525)
                     /locus_tag="PLP58_09125"
     CDS             complement(4293..5525)
                     /locus_tag="PLP58_09125"
                     /inference="COORDINATES: protein motif:HMM:NF012694.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HRJ08717.1"
                     /translation="MPTFAYSAHSPSGVITGELSAADRAEAISLLGRKKLRPFKLEQS
                     GAQSVREAAAASAQSAPQGPLKIKIAQVTHFIEELSDLLSAGIQLEPALATMERRREL
                     SGIKTLATALRAKVRDGMPFSKAVSTTTPSFGNLFCALASAGEASGTLPSVLKRQAAY
                     MRSLLALRSKVAFALIYPAFLIVSAVAVTLLFIVYLIPKLTELLDATGGSLPLGAQII
                     LKLSELFKATWWMAGGGLFAAWLLLKAWLSQPASRIPWARFLLRLPLFGGVLKARFHV
                     QFLETLANLLGNGLPLVHAMQLTHQAIENPFYQREFEGVMRHVAEGVSLSRALARSPH
                     FPDLLIDIVSVGEQTGDLAAALGKAAERFDRELTKKVEKLSALVQPLIVCLMAGMVGI
                     MAYLMITTIFQTISGMSQ"
     gene            complement(5562..7358)
                     /locus_tag="PLP58_09130"
     CDS             complement(5562..7358)
                     /locus_tag="PLP58_09130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009962525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GspE/PulE family protein"
                     /protein_id="HRJ08718.1"
                     /translation="MMPLIQQMLGEAGCADSPAVRQAVDEAGFNQVSFVEPVLDCPEV
                     RERDFLQVLARTLELPWWEGGGETPPDSSLRKHLPAELALRHRILPVGFAREEHEDES
                     GHEARATLHLATFDPLNLVTHQRVAATVTHPVAWHVGQRTRIVEGIQRLYGLGADTFE
                     KILRGRAEWEGEEAGDEVTVLDEPEDEEASVVRFVNQILRRGLEQRATDIHVEPQQDR
                     LRIRCRVDGRLEELPVPENIKSLQASVIARLKIMARLDIAEKRLPQDGRINLELDGQP
                     IDVRVATIPSVEGESISLRLLSQQVMTLDRLGLTGGLRPVIEDLLRLPNGIILITGPT
                     GSGKSTTLYAFLSAMNQPHRRIVTIEDPVEYKMPGMVQIAVKPEIGLTFATGLRSILR
                     GDPNVVMVGEMRDLETTEIAIRAALTGHLVFSTLHTNDAIGGITRLVDMGVEPFLVSS
                     AVRAFFAQRLVRKLCPLCKAPAEVEREYLQSIGFPLSAAGRIHRATGCEACRGSGYQG
                     RLSIYEVVQLTPALQHLINIRSHPAELKKQAMEDGYLPMRGYGFQKVLNGETTIEEVL
                     SVTAMAEGENSKSETRNPVSGPLSAASAISVH"
     gene            7669..9333
                     /locus_tag="PLP58_09135"
     CDS             7669..9333
                     /locus_tag="PLP58_09135"
                     /inference="COORDINATES: protein motif:HMM:NF013697.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HRJ08719.1"
                     /translation="MADSADSSSPHRWRKELLILGALAVTLAGPFLLKPRDSTASTNF
                     DRRLVILTPHPERLRAEFGQAFARHWKEKTGETVAMDWRVPGGTSEIAVLLKSEYSAA
                     FQNLWKREMGREWAPQVAQNFMSGKAPADDPARAAFLASNAGIGVDIFFGGGAYDFQI
                     QAEAGTLVAQSGDATGIAALKEKHPAWFGDEGIPEMVSGEPFRDAKDRWTACCLSSFG
                     IVFNRDVLRRLGIEKDPRQWRDLADPRLHGQLALADPGRSATVTKAFEMLIQQEMQEA
                     VNRLAEKPGRLSTAEEIEKEGVRQGWLAGLSLIQRISANARYYSDSSSKIPLDVARGD
                     AAAGMCIDYYGRSTEEETRRADSTSRVGFIMPVGGSSVSVDPIAMFRGAPDPELATAF
                     MEFVLGDAGQGLWAFKAGAPGGPQRSALRRLAARKDFYTPENLRHMSDADEMPYEKAK
                     AFTYHPEWTGSAFSTLRFLIRVICVDAHHEQRAAWREIFGNDLPKRATEVFHNLDAVH
                     YDLALGEINSVVRSRDKVAETRLARDLGGVFRRNYELAARLARKGE"
     gene            complement(9349..11442)
                     /locus_tag="PLP58_09140"
     CDS             complement(9349..11442)
                     /locus_tag="PLP58_09140"
                     /inference="COORDINATES: protein motif:HMM:NF014825.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SEC-C metal-binding domain-containing protein"
                     /protein_id="HRJ08720.1"
                     /translation="MTPELRDFVSTHKFERVHIEKLAEMLPQDDGEFDTLIAGVVDKN
                     DWNAFTFLVTAALGAGRFVDGRHLREGTCLAPNGTYLGTFFWHMRGDSKFDSLVHALC
                     KHKLATEIQLHGLLAAAGWCKTHHEGKWPDDLLRSCREIMRRKMSNDKPRHLLHALAA
                     YIDDPDLIMLAHEHHGKIQLDDELHQCAVKVAEAHLAVYQLPVMGMVPSTIRSLGAGT
                     HLRRSIPKISRNAPCHCGSGQKYKRCCHDKDQERAHSFSEVEGKTPAELEESREPHLT
                     PDNIQKLSRAQVRKLDPMKISHDILPWYFLIIGSHGLFDEAASAFEKLGWLDHVTNFD
                     AAWDNVVTFATWAGLPEVAERLIRARYPDGVVPEGVLKPGTELLRLHSCPDLYLAQLE
                     KMALEALTCKESDRQQSLAYGLLSPLHPALSLLMVQGMLPVISKQKAFKLLEFMQKHR
                     DQLLLPAEDPFTEILERRFMDAAQASHGKDAQKLREANDRLQVKSSQVNELRGQLETM
                     RRELRLKEKAAKRETTAAAAPTSAELEALRELREKVERLKSTIQDHSQERAALRHDLA
                     SAYTELQELRRQKSAQNPAETSNDADDESLTLPATLEDAQPVRLIEYPKKFHATLSSL
                     PKHVSRSAQVLLGRLSAGEPSAFVGIVALRARPDTLRLRVGADHRLVFRLHPASLEVL
                     DLINRRDLDRLVKSL"
     gene            complement(11548..12735)
                     /gene="nspC"
                     /locus_tag="PLP58_09145"
     CDS             complement(11548..12735)
                     /gene="nspC"
                     /locus_tag="PLP58_09145"
                     /EC_number="4.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009962316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_process: GO:0008216 - spermidine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxynorspermidine decarboxylase"
                     /protein_id="HRJ08721.1"
                     /translation="MSFTPPHVFEQPAPAFDLSQVETPAFVVDLGLLQKNLEKLARVQ
                     EASGASILLALKGFSMFAAFPLVRQFLRGCCASGIHEALLAHEEFGREVHVYAPAFKV
                     AEMREIIPIADHISFNSPTQWRKFRPMLDAARAAGKRAPSPGIRVNPEHSEVEVSLYD
                     PCSPGCRLGTRADALENESLDGLEGLHFHALCEQDSDVLERTLAAVERRFPRLLEKVK
                     WVNMGGGHHITRNDYDVERLVRVVRGFKERWGKEVYLEPGEAVALNTGWLIATVMDIV
                     PTDIPTAILDTSATAHMPDTLEMPYRPHILGSDEPGKLAHTFRLGGLTCLAGDVIGEY
                     SFPAPLEPGQKLVFTDMAHYTMVKTTTFNGVPHPDIVLHDPAAGLRLVRRFGYEDFKR
                     RLS"
     gene            complement(12800..14926)
                     /locus_tag="PLP58_09150"
     CDS             complement(12800..14926)
                     /locus_tag="PLP58_09150"
                     /inference="COORDINATES: protein motif:HMM:NF024909.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BatA domain-containing protein"
                     /protein_id="HRJ08722.1"
                     /translation="MTFLNAILLAGASAFLIPLIIHLLNKRRVITVRWGAMHLLHEVV
                     RQRKRRMKIEQWLLLAVRVSIPIVLALCLARPVLTALRSFGLGQTSLVMLLDDSFSMR
                     APSSGGTPGERAREDIAGILQDLPGGSDAQVVLAGGNARTLFEDPVTALEELPGQLAE
                     SSRLAGPVRVNDALQAAAAALAKSQNAARELAVISDFQAADWQGAADGAALPALDALL
                     KQEPAPQVTFYRISSDLAENLSIASAELSALVLAEGQPAGLRARIVNHGKKPWQDVAV
                     HLEADGARLRSTRVSLPAEGESIVNFTHAFETTGEHSLAVRIEGDSFPDDNAFYSVVQ
                     VRDQLNVLLVDGDPSTTPLGGAADFLELALAPYQSASASLKDLIRATKVDARRFREED
                     MRGKEVVILADVERLQGNRMADLDKFVSAGGGLIVFAGPHCDLEWYNRDFFKKGEGLF
                     PAAISGLKRAQGMPARILQQRLTHPAVLYFNDARGGRLQDAEFQSWFELDAVTEAKPL
                     LQLDQGVPLIVEKQHGSGRVIAMATTADAEWSNLPLQPFYVPLMQRLAAHLATQSTST
                     AWIPAGTPTRLTVPRELASAEHSLRDPAGLTSTLTPIVEGGNTMLKTPPLETPGIYQL
                     VAGGRTRLFAVNLDTAESAITPMPAENVRRIAERHGAEFVESLDAWRKLDRTRRFGSE
                     FWQPLLLALLGLLFFEVLLQQRITRG"
     gene            15071..15868
                     /locus_tag="PLP58_09155"
     CDS             15071..15868
                     /locus_tag="PLP58_09155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009965035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRJ08723.1"
                     /translation="MNLRVISALVLRYIFLYTRNPIRLVEMIFWPLVDLLVWGNLTLF
                     LQRNTTTEFGGFILFLLGAMILWDILFRAQQGVAISFLEDVWTRNLLNVFVAPVRTTE
                     YLAATFAVGMLRIFVTVAVLGLVAWAGYRFDIFILDWWLLPFFLNLMVFGWSLGMIST
                     ALILRWGQAAESLAWAVPFFIQPIVAVFYPVADMPAWSQPFAWCFPATYVFEGMRQVM
                     NGGVMDMGLLVKAVLLNAGYLAAAGALFFHILRVTRKRGLLTKFATQ"
     gene            complement(15923..16906)
                     /locus_tag="PLP58_09160"
     CDS             complement(15923..16906)
                     /locus_tag="PLP58_09160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ08724.1"
                     /translation="MKTLVTKSTTLLCAAALCAAAALSTPRTASAFGNVVESTRPRVI
                     LASPNASAPRTVITLLGDGLRRDKDGNLWTGRAPYQIQFPTAAPGQFVNAAFTFVSTN
                     SLRVTVPASAVSGRIRLVQQEYVTSTLFSFTVNNPLPAQQSRLNMQNNSQYNAISLRV
                     NGVEQFAQGNGIPPGRLQEGIHTPGRKTVVVEIGINPGEPLFNFTFNAFLTAGRTTTL
                     TIPRVTLAQLMTNFLASRDYKSDVLADNNGNLYQRTVRFNANGSYQIIDSTGSKPVTV
                     ETGRYTETAWADNSIQVSFRLGTRAVDIFHPFAQFISNVGRNNQRIVVTAQ"
     gene            complement(17039..17194)
                     /locus_tag="PLP58_09165"
     CDS             complement(17039..17194)
                     /locus_tag="PLP58_09165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ08725.1"
                     /translation="MTTAGLIVMLCSVGTVVSLFGWCVWKVLGTPDEAGKIHSLEFET
                     PDAKRPE"
     gene            complement(17238..18974)
                     /locus_tag="PLP58_09170"
     CDS             complement(17238..18974)
                     /locus_tag="PLP58_09170"
                     /inference="COORDINATES: protein motif:HMM:NF012435.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ08726.1"
                     /translation="MSSKKEAWGSRLGVIMAVAGSAVGLGNFLRFPGLAAQYGGGAFM
                     LAYAISFLIIGLPIGWAEWAMGRHAGGRGYNSCPGAFAAIVRRPWAKYAGIIGVIVPV
                     VIYMYYVVIESWCIGYAVNFWSGGLRLENSGQTVARFAEFTGAAADGSAMSFDSGKVL
                     PWLLGVFILNFWLIYRGISGGIEKMCKIAMPMLILLALILLARVLTLGAPDAARPNDS
                     VGNGLGYLWNPTKVMVVHTAPDAKDKPREVVGAAAIEAAKTEVAASGGALALREAGVL
                     DQLRNPRLWLAAAGQIFFSLSVGFGVIIVYASYMKKSDDVVLSGLTASSANEFCEVGL
                     GGLITVPAAVAFLGVAGVAGQAGVGLGFKVLPLVFAKMPAGDFFGGAFFFMLFLAAIT
                     SSISMLQPGIAFVEEALGVGRKVSVTLLGLLTCFGAGFVIYFTGDLKALDTLDFWVGT
                     FLIFMLATIQIIVFGWVWGIDRGFDELHQGAAIQVPWIFRPVIKWICPAFLLGIFALW
                     FMQEILGMDLSSGAAGGVSYYVTDLFGEKASLPAQLSVGLVIVIAVFFSLMTSRARAF
                     QRAEQGLDKDDV"
     gene            19167..20963
                     /gene="lepA"
                     /locus_tag="PLP58_09175"
     CDS             19167..20963
                     /gene="lepA"
                     /locus_tag="PLP58_09175"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018969080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor 4"
                     /protein_id="HRJ08727.1"
                     /translation="MSIARTRNFSIIAHIDHGKTTLSDRLLEFTKTITERQQQDQLLD
                     SMDLERERGITIKAHPVCMNYKAKDGHLYKLNLLDTPGHVDFSYEVSRSLAACEGALL
                     LVDASQGVEAQTVANLNLALQQNLHVIPVINKIDLPSADVDKVKRQLEDILQLPADEA
                     VSASAKMGIGIEDILEAVVAFIPPPKEPGDGYLRASVFDSVFDAYRGVVSYVRVVSGT
                     IQRGQKVRMMSTGNDYEIKDVGIFRPKMTSCEKLEAGDVGYLIANVKSTADVKIGDTL
                     TESRRPAPEPLPGFKEIHPLVFSGIYPVSTDDFEALKLAVGKLQINDAAFTFMAESSA
                     ALGFGFRCGFLGLLHMEIVQERLRREFNMDVISTYPSVIYEVVKTDGTELLVDNPSFL
                     PAANEIEEIREPIVKVYIMIPNEHIGDIMQLVMDKRGQVNNTETLDDKRVMLHTVLPL
                     NEILVDFNDKLKSLTRGYGSMDYEHAGYQAADLVKMDMLIASEPVDAFSCIVHRSKAE
                     SRGRQLAAKLKEVIPQQLFVVAIQAAIGGKVIARESISALRKDVTAKCYGGDITRKRK
                     LLEKQKEGKKKMKAIGRVNIPQEAFIEVLKTN"
     gene            21165..22421
                     /locus_tag="PLP58_09180"
     CDS             21165..22421
                     /locus_tag="PLP58_09180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009962482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HRJ08728.1"
                     /translation="MNEDRSLSRTAWMVVALLFPVALLNYLDRQMIAAMKVSVMGDIQ
                     DIGSDENWGHMLASFKWVYAVFSPLGGYIADRFSRKLTICSSLFVWSLITWLTGHVSS
                     FQELYWARTAMGISEAFYIPAALALIADYHTGGTRSRAVGVHQMGIYCGQMLGGFAGF
                     SDRLEGGWRSLFDVTGLTGILYAIPLLFLLRDAPSKPRPDVNGGPAAQTRAGTGIVEL
                     LTNRNFILLVAYFTLPAIAAWVVRDWMPAILQKAFDISQGKAGVSATLYWQAAALLSA
                     IFGGWLADRWMRKSIRGRIHVSAIGMMLIVPAMFGVGNAPALNSFALAIVALVLFGVG
                     WGFFDCNNMPILSQIVRPELRATGYGIMNFVSMMCGGVADWGFGAMTDRGVPLNVIFG
                     VFALVCVASLFIVLLIRPRETPGGQG"
     gene            22477..22890
                     /locus_tag="PLP58_09185"
     CDS             22477..22890
                     /locus_tag="PLP58_09185"
                     /inference="COORDINATES: protein motif:HMM:NF017902.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF family regulator"
                     /protein_id="HRJ08729.1"
                     /translation="MTQLLAPAIASRIDELCAAIAADPDVQKARDQAEAFLADEDAVG
                     LYREVMNLGRSLEQRHRGGQKLDAQEVSRFQDLQDQADAHEGIQAFVAAQDVLQEVAN
                     RVNGFVTKTLEKGRVPTQEEVFGQGGCGEGCGCHH"
BASE COUNT         4066 a         6908 c         7640 g         4311 t
ORIGIN      
        1 gcgcgcgcga gcggcttcgc gcggcagcgc gcgcgtgcaa aatctccggc ggcatcaaag
       61 gcgacacacc atgatttggc atcaccggcg cggggcgctc catgcgcgcg gcgggcaggc
      121 cggtgacgcg cgtgacttca tcgagattgc catcttccag ccagagctgg atctcgcgca
      181 cgcggcccag cacgccctga aagccgagct gcacgcagat gaggttcacc tcgccaaggg
      241 cgcgggcgtc caactcgcgc gcggaaagcg ggctggcata gatgacctcc gcgccgtggg
      301 tgacgaggat gaccaggcgg ccaagctcgc gcaaaagcac aatgccatcc tccggcagcg
      361 gcaggcaggc gaaatgcggc agcacttcgt gcggcaggcg cgaggcgtcc caaagcggcg
      421 cgccggtctc cttgagcgcg gtgatgcgtg tgaggatgcc gccctcgcgc gccatgatct
      481 cccgcacatg caggccgtgc tccatgtcgg ccacgcgcac gccgaggcgc tccagatgca
      541 gcgcggccat gtctttgaga tgcgcggact caccctccat ccatgcgggc agcacccaca
      601 gatgcgcggc aggcagcgcc agcgcgcggt cctggctgtc cctggcgaaa acgcccgcgc
      661 tctggccgaa ctcggcggct ttcacacagc gctcacccgg cgcgccggtc cagcgttccc
      721 agccggtggc acccggcagc agcaaatcgc gcgtctgttt acgagtcctg cccatgcggc
      781 acctcccctc tccaaatggc ttgttggtct tgtacgatga gcacaaggcg gcggcgcaca
      841 tcgccaaaag tcccggtgct ttcgatgcgc cggatgggtc cggcaaactg cgtccagcgc
      901 gtcaggtgct cgacgatctg cggttgaaaa atgccaagca actgcgccac ctgcacggcg
      961 gattgcagcc gcacatcgtc ctgcgtgtcg cgcacgccat cgcgacccgc gcgcagggtg
     1021 agcagcggtg ccaggcgctc catgggcaca ttggcgagca gggaaatgag ttccggacgc
     1081 gcgtcattca catccacgcg gccatcaccg agcacagtga accactcgcg ccagtcgggc
     1141 cgcaaggcgt ccacgatgtc catgccgcgc acgaggcgca tttcctccac ctccttgaag
     1201 ggacggttga agggcagccc gatgaggccg gccttttcat agtcgcgctt ttccgcgcca
     1261 ttgagccggg tgcggtcgtc ggcgtccacc cagtcgtgca gggcatcatg cagggcgctg
     1321 gcatcctccg ccttgagtcc ccaggaggca aaaagacgcg gcagcagcac gcggtcatcg
     1381 gagcctaaca agacattgat gttcagcctg gcctcctcgg cgacgatgcg cacctggtag
     1441 gagccgccat ccggggtttg ataagagagc agcggatcat gcgccggcag ggcagggtgg
     1501 cgtccgattt ccaaacccat ctgcgccagc cgcttcgcat aaacccttcc gcgcatgagg
     1561 cgcgtgtggc gcgcgtccgt gtcgagcgcc ctggcccccg catacacgag catgcccatg
     1621 accgcgatca tccaaaacac cgcgatgagc gcggagcctt gatggcgggc ggggcgttgg
     1681 cgggggaggc gcatgggtta gacgggcaga tttgaagttc gaagttcgaa gttcgaaatt
     1741 tgaagaagga ggggaaccac gaatggacac gaatggacac gaatggtgtg tggtctcggg
     1801 agtgcttctg tggtgtggag ggttctggct aggctcagtg ttggcatgcc atagcctgcc
     1861 aaggcggctc tttgtctttt ctgattcgtg ttcattcgtg tccattcgtg gtttcccaaa
     1921 ccctcttgag atgaattgac gttcatgacg gttttgaggt cgttggcgat ccagaagacc
     1981 agctcatgct ctcgtggatc atccaggaag gcgaagttca ggcgcatgaa gagcggcggg
     2041 gcggggcgtt tgtcggggtc ccaactggcg aaccattttt tctctccggc gtcgtaatag
     2101 ctggcgcgga acgttttcag attttgcagc agcggcagct cggccatgag ctggtaattt
     2161 tccagtgggg ttggcttttc gaggcgcttc aaatgacgca cgagcagagt gagcgacttc
     2221 tcacgatccc cgcgcacgac ggcaaactcc acggcatccg cgagggcgag cgcctgggac
     2281 caggcaaagg gcgcgggtcc agcctcaatg agcagagcgc ctgcggagcc gcgcgcgcct
     2341 ttttccagcc cggcgctgag gcggatgccc gggggcaggt tttccagata atcccgccac
     2401 gtcatggtga agtggctcac gcgtgtctcc atgaggcggg ccctcgacat ggaggctccg
     2461 agccgcagtg agccatcggc gatgccatag accccgccga ggatgagggt gatgaggccg
     2521 agggccatga tgacctccag gagggtgaag ccttgaggcg caaacatcgt gcctgagcga
     2581 acaggcgaga cgcctgttcc ccatgctttt ctgcaagccg aagccagctc agggcgatgc
     2641 gggtgagtac acataggtct gcatttggcg cttgagcact tttttccggc catcctcgat
     2701 ccatgcctcg gcctggatgt ggaagatgtg gtcgagctgg gttttgtctt tggtgatgag
     2761 ttcggctttg gagatggtgg cgcgggcttc gacgtggtcg gcggatttgg gaaaggtgac
     2821 ggtggtgggt ttcaactcgg gctggtggct gacttcggcg agcacggact cgagcacgcg
     2881 cagcacctgc gcctcctgcc gcgcctgccg cgaggtctgc gcgatcatgt cgagcgcgcg
     2941 ggtgagtccg acggcgacca atgagaagag gccgagcgcg aggatgattt cgagcagggc
     3001 gaagccgtgg gaatgttgga gtgaagccgt tggagtgaag cctttaggcg acacgggagt
     3061 tggctccgct gcggagtcca ctcccggaaa agctaaagct tgaactccaa cgtgtggact
     3121 ccaacgttgg agtgaagcct ttaggcggca cgggagcgga ctccgctgtg ggcggtcagt
     3181 gaacaagctg aagcttgaac tccaacggct tgaaatgtca gcgcggtgtt tcatccgttg
     3241 acgatgacgt ttttcttgat ggcgcaggcg gtgaggggat cgaagccaag ctcgatgatg
     3301 cctgccgggc tggagaggcg gacttcgagc ggctcgcaaa tgccggtggg gttgaactcc
     3361 cagacctcgc cccttttggg tgcgcggaat tctttttcac catgacgctt gatgagcatc
     3421 tggccttcga tgccgagccc gcctggcaag ccaagaacga tggtgcgctg gctggtgacg
     3481 gcgcgtagga gggactcgcg cgcggtggtc tcgatctggg aggcgctccg cttcaagccc
     3541 gcctccgcct gcacgccgct gatgcccagc acggccacgc cgaaaatgag cgtggcgagt
     3601 gtgagggcga ggcagacttc gaggagggtg aaggcgggtt tcatttcggg gatttgggaa
     3661 ccactaatgg actctaatgg gcgctaatga tggatggggc ttggaggact catggaatgg
     3721 gctgacgtat ttgaatttta gtcatggggc attcctcaga gatcccagtt gccgatgtcg
     3781 tcggctgtgc cgggggtgcc gtcctcgccg gcggagaaga catcgaagcc gcctttgtcg
     3841 ttctggccgg ggcggcggta gtggtagggg ttgccccagg ggtcgaggag gacctttttc
     3901 agcttgggcg cccagttctg cggcgcgggg cgtgaggagg gcttttggac gagggccatg
     3961 attccctgct cgctggaggg caggaacatg ttgtcggtct catacgagcg gagggcggcg
     4021 ctgagggtgt tgaggtcgcc tttgacgcgg gtctttttgc cggagtcaag cacgccgacg
     4081 agggagacga tgccagcccc gaccagcagg gccacgatgc cgaggacgag caccatttcg
     4141 acgagggtga aagcggcggt gcggcgtgga tggatgcggt gggttttcat gggatgcagg
     4201 attttttgga accactcatg gacactgatg ggcactaatg agcgatgggt tggggtttga
     4261 atttgggatt tcgactttcg gctttcgttt ttttactgac tcatgccgct gatggtttgg
     4321 aagatggtgg tgatcatgag gtaggccatg atgcctacca tgccggccat gaggcagacg
     4381 atgagaggct ggacgagggc gctgagtttt tcgactttct tggtgagctc ccggtcgaag
     4441 cgctcggcgg ctttgcccag ggcggcggcg aggtcgccgg tttgctcgcc gacgctgacg
     4501 atgtctatga gcaggtctgg gaaatgcggg ctgcgggcga gcgcgcggga gaggctgacg
     4561 ccttcggcca catggcgcat gacaccctcg aactcgcgct gataaaaggg gttttcgatg
     4621 gcctgatgcg tgagctgcat ggcgtggact agcggcaggc cgttgccgag gaggttggcg
     4681 agggtttcga gaaactggac gtgaaaacgc gctttgagca cgccgccaaa cagcggcaga
     4741 cgcaggagga agcgcgccca agggatgcgc gaggcgggct gggagagcca ggctttgagc
     4801 agaagccagg cggcgaacag accgccgcca gccatccacc aggtggcttt gaaaagctcg
     4861 ctgagcttca agatgatctg cgcgccgaga ggcaggctgc cgcctgtggc atcgagcaac
     4921 tcggtgagct tggggatgag atagacgatg aaaagcagcg tgaccgcgac ggccgagacg
     4981 atgagaaagg cggggtagat gagggcaaag gccaccttgc tgcgcagggc gagcagggag
     5041 cgcatgtagg cggcctgacg cttgagtacg gagggcagcg tgccgctggc ctcgccggcg
     5101 ctggcgagag cgcagaagag atttccaaag ctgggtgtcg ttgtggagac ggctttcgag
     5161 aagggcatgc catcccgcac cttggcgcgc agggcggtgg cgagtgtctt gatgccggaa
     5221 agctcccgcc gccgctccat ggtggcgagc gctggctcga gctggatgcc ggcgctcagc
     5281 aggtcggaga gttcctcgat gaagtgggtg acttgggcga ttttgatctt caaggggcct
     5341 tggggggcgc tctgggcgga cgcggcagca gcttcgcgta cggactgcgc gccggattgt
     5401 tcgagtttga aggggcgcag ctttttccgg cctaacaaag agatggcctc cgcgcggtcg
     5461 gcggcactaa gctctccggt gatgacaccg gaggggctgt gggcgctgta agcgaaggtg
     5521 ggcatgggga ttgcgaggag ttttggaacc actaatggac actaatgaac actgatggcg
     5581 gatgctgcgg agagggggcc ggagactggg tttcgagttt cggatttcga attttcccct
     5641 tcggccatgg cggtgacgct gaggacttcc tcgatggtgg tctcgccgtt gaggactttt
     5701 tgaaagccgt agccgcgcat gggcaggtag ccgtcctcca tggcctgctt tttcagctct
     5761 gcgggatggg agcggatgtt gatgaggtgc tggagcgcgg gtgtgagctg gacgacctcg
     5821 taaatggaga gccgcccctg gtagccgctg ccccggcagg cctcgcagcc ggtggcgcgg
     5881 tggatgcggc ctgcggcgct gagcggaaag ccgatgctct gaagatactc gcgctcgacc
     5941 tcggcgggcg ctttgcacag cgggcagagt tttctcacca aacgctgagc gaagaaggcg
     6001 cgcacggcgc tggacacgag gaagggctcc acgcccatgt ccacgaggcg cgtgatgccg
     6061 ccgatggcgt cattcgtgtg cagcgtggaa aagaccaggt gcccggtgag cgcggcacgg
     6121 atggcgatct cggtggtctc caggtcgcgc atttcgccga ccatgaccac gttcgggtcg
     6181 ccgcgcagga tgctgcgcag gccggtggca aaggtgagac cgatctccgg ctttacggcg
     6241 atctgcacca tgccgggcat tttgtactcc acggggtcct caatggtgac gatgcgccgg
     6301 tgcggctggt tcatcgcgct gaggaaggcg tagagcgtgg tggacttgcc gctgccggtg
     6361 gggccggtga tgaggatgat gccattggga aggcgcagca ggtcctcgat gacgggacgc
     6421 aggccgccgg tgagaccgag gcggtcgagc gtcatcacct gctgggagag caggcgcagg
     6481 ctgatgcttt cgccctcgac gctggggatg gtggccacgc gcacgtcaat gggctggccg
     6541 tcgagttcga ggttgatgcg gccatcctgc ggcaggcgtt tttcggcgat gtcgagccgc
     6601 gccatgatct tcaggcgggc gatgacgctg gcctggaggg acttgatgtt ttccggcacg
     6661 ggcagctcct ccaagcgtcc gtccactcgg cagcggatgc gcagtctgtc ctgctgcggc
     6721 tcgacatgaa tgtcggtggc gcgctgctcc agaccgcggc ggaggatttg attcacgaag
     6781 cgaaccacgc tggcctcctc gtcctcgggt tcgtcgagca cggtgacttc atcccctgct
     6841 tcctcaccct cccactcagc gcggccgcgc aggatttttt caaaggtgtc cgcgcccagc
     6901 ccatagagcc gctggatgcc ctcgacgatg cgcgtgcgct ggcccacatg ccaggccacg
     6961 gggtgcgtca ccgtggcggc cacgcgttgg tgggtgacga ggttcagcgg gtcaaaagtg
     7021 gccagatgca gcgtggcgcg cgcttcatgg ccggattcat cctcatgctc ctcgcgcgcg
     7081 aaccccaccg gcaggatgcg gtgccgcagg gcgagttcgg cgggcaggtg cttgcgcaaa
     7141 gatgagtcgg gcggggtctc gccgccgcct tcccaccagg gcagctccag cgtgcgcgcg
     7201 agcacttgga ggaagtcgcg ctcccgcacc tccgggcagt cgagcaccgg ttcgacgaag
     7261 gacacctgat tgaagcccgc ctcatccaca gcctgccgca ccgccgggct gtctgcgcaa
     7321 ccggcttctc cgagcatttg ctggatgagg ggcatcatgg atgaaggatt ataggggcaa
     7381 tttcagtgta ttgaattcag aaattatggt ttttatgcag aaatgcaaga attaagtctt
     7441 tctgaatcat ttggagttcg agagttttga gagtgccgtc tggccctccg agtggtgagt
     7501 ttgggctgtg tgtttttcaa agcggctgaa gcctgggcac ctccattgga ttgaagccgt
     7561 caggcgactc atggtaggac gccgctgcgg gagttcattg aacaagctga agcttgaact
     7621 ccaacatgcc tgttgccgcg cgcgggttcc tccgcttgaa tcgccgggat ggcagactcc
     7681 gctgattcct catcccccca ccgctggcgc aaggagctgc tcattctggg cgcgctggcg
     7741 gtgacgctgg ccgggccgtt tttgctgaag ccgcgcgact ccaccgcgtc cacgaacttc
     7801 gaccgcaggc tggtgatcct gacgccgcat ccggagcggc tgcgcgcgga gttcgggcag
     7861 gcatttgccc ggcattggaa ggaaaaaacc ggcgagacgg tggcgatgga ctggcgcgtg
     7921 ccgggcggca cctcggagat cgccgtgctg ttgaagtcgg aatacagcgc cgcctttcaa
     7981 aacctgtgga agcgcgagat gggccgcgag tgggcgccgc aggtggcgca gaatttcatg
     8041 agcggcaaag cccctgcgga tgacccggct cgcgcggcct ttttggcgtc gaatgcgggc
     8101 attggcgtgg atattttctt cggaggcggc gcctatgatt ttcaaatcca ggccgaggca
     8161 ggcactctgg tggcgcagtc cggtgatgcc acgggcatcg ccgctttgaa ggaaaagcac
     8221 cccgcatggt tcggtgatga gggcattccc gaaatggtca gcggcgagcc ttttcgcgat
     8281 gcgaaggacc ggtggacagc ctgctgcctt tcgagcttcg gcatcgtttt caatcgcgat
     8341 gtgctccgcc gcctcggcat cgagaaagac ccgcggcagt ggcgtgacct ggccgacccg
     8401 cgtttgcatg ggcagctcgc gctcgcggac cccgggcgca gcgccacggt gacgaaggcc
     8461 tttgaaatgc tcattcagca ggagatgcag gaagccgtca acagactcgc ggaaaagccc
     8521 ggtcgtctga gcacagccga ggaaatcgaa aaagaaggcg tgcgccaggg ctggctggcg
     8581 gggctttcgc tcatccagcg catcagcgcc aacgcgcgct attatagtga cagctccagc
     8641 aagatcccgc tcgatgtggc gcgcggcgac gcggccgcgg gcatgtgcat tgactattac
     8701 ggccgctcca cggaggagga gacgcggcgc gccgacagca cctcccgcgt gggcttcatc
     8761 atgccggtgg gcggcagctc ggtgagtgtg gaccccatcg ccatgtttcg cggcgcgcct
     8821 gatcccgaac tggccacggc gttcatggag ttcgtgcttg gtgacgcggg gcaggggctg
     8881 tgggccttca aagccggcgc gccgggcgga ccgcagcgca gcgccttgcg caggctggcg
     8941 gcgcggaagg atttttacac gccggaaaac ctccgtcaca tgagcgacgc ggacgagatg
     9001 ccgtatgaaa aagccaaagc cttcacctac catcccgagt ggacgggctc cgccttcagc
     9061 acgctgcggt ttttgatccg cgtcatctgc gtggatgcgc atcacgagca gcgcgccgcg
     9121 tggagggaga tttttggcaa tgacctgccg aagcgcgcca ccgaggtttt tcacaacctg
     9181 gacgccgtgc attacgacct ggcgctgggc gagatcaaca gcgtggtccg ctcccgcgac
     9241 aaggtggccg agacacgcct ggcgcgcgac ctgggcggcg ttttccgccg caactacgag
     9301 ctcgccgcgc gcctggcgcg caagggggag tgaaggtcgc gcgcattttc aaagcgactt
     9361 caccaggcgg tcgagatccc ggcggttgat gaggtccaac acctccaggc tggctggatg
     9421 caggcggaag accagcctgt ggtcggcccc cacacgcagg cgcagggtgt ctggacgcgc
     9481 tcgcagggcg acgatgccga caaaagcgga aggctcgccg gcggagagcc tgccgagcag
     9541 cacctgggcg gagcggctga catgctttgg cagactgctc agcgtggcat ggaatttttt
     9601 gggatattcg atgagccgga ccggctgcgc gtcctccagc gtggcgggca gggtcaatga
     9661 ctcgtcgtcc gcgtcgttgg aagtctctgc ggggttttgc gcgctctttt gccggcgcag
     9721 ctcctgaagc tcggtgtagg cggaggccag atcatgacgc agggccgcgc gctcctggga
     9781 gtggtcctgg atggtggatt tgagacgctc cactttttcc cgcaattccc gcagcgcctc
     9841 cagctcggcg cttgtcggcg cggcggcggc cgtcgtctcg cgcttggcgg ctttttcctt
     9901 gaggcggagc tcccggcgca tggtttcaag ctggccgcgc aactcgttga cctggctgga
     9961 cttgacctgc aggcggtcgt tggcctcgcg gagtttttgc gcgtctttgc catggctggc
    10021 ctgggccgcg tccatgaagc gccgctcaag gatttcagtg aagggatcct ccgccggaag
    10081 caggagctgg tcccggtgct tttgcatgaa ctccaggagt ttgaatgcct tctgcttgga
    10141 gatcactggc agcatgccct gcaccatgag cagggagagg gctgggtgca gcggcgagag
    10201 caggccgtag gccagcgact gctggcggtc gctttctttg catgtcaggg cttcaagagc
    10261 cattttctca agctgcgcca ggtagaggtc aggacatgag tgcagcctga gcagttcagt
    10321 gcctggcttc aggacgcctt cgggcacgac gccgtccggg tagcgcgccc tgatcagtcg
    10381 ctcggcaacc tccggcagac ccgcccatgt cgcgaaagtg acgacgttgt cccacgccgc
    10441 gtcaaaattg gtgacatgat ccagccagcc cagcttttca aaagctgagg cggcctcatc
    10501 aaaaagtccg tgcgagccga tgatgagaaa ataccacgga aggatgtcat gggagatttt
    10561 catcgggtcg agttttcgca cctgcgcccg gctgagcttt tgaatgttgt ccggcgtgag
    10621 atgcggctcc ctggattcct ccagttccgc cggtgtcttg ccctcgactt cggagaagga
    10681 gtgggcgcgt tcctgatcct tgtcatggca gcagcgtttg tatttctggc cgctgccgca
    10741 gtggcagggg gcgttgcggc tgattttggg gatggagcgg cggaggtgag tgccggcgcc
    10801 aagagagcgt atggtggaag gcaccattcc catcacaggc agctgataaa cggccaggtg
    10861 tgcttcggcc actttgacgg cgcattgatg gagttcgtcg tcgagttgga ttttcccatg
    10921 gtgctcatgc gcgagcatga tgagatcggg atcgtcaatg tatgccgcca gggcatgcag
    10981 gagatgcctg ggtttgtcgt tgctcatttt gcggcgcatg atttcgcggc aggagcgcag
    11041 caggtcatcg ggccactttc cttcgtgatg tgttttgcac catcccgcgg ccgccagaag
    11101 tccgtgcagt tggatttctg tcgccagctt gtgtttgcag agggcgtgga ccaggctgtc
    11161 gaatttgctg tctccccgca tgtgccagaa aaaagttccc aggtaggttc cattcggggc
    11221 gaggcaggtg ccttcacgga gatgcctccc gtccacgaac cggcccgcgc cgagcgcggc
    11281 ggtcacgaga aatgtgaagg cgttccaatc gttcttgtcc acgaccccgg ctatcagggt
    11341 gtcgaactcg ccgtcgtcct gcggcagcat ctccgccagc ttttcgatgt ggaccctttc
    11401 gaatttatgg gtgctgacaa aatcgcgcag ttcgggtgtc atcttcatgg catggggtga
    11461 ggcggggact cacagcctga aattaatgat attccggcag tgatgggaag ccggaagttc
    11521 tgtttggcgc gcccggcggc gcgcgcctca ggacagccgc cgcttgaaat cctcgtagcc
    11581 gaagcggcgc acgaggcgca ggccggcggc gggatcgtgc aggacgatgt ccgggtgcgg
    11641 cacgccgtta aaggtggtgg tcttcaccat ggtgtaatgg gccatgtcgg tgaagacgag
    11701 tttttgcccc ggctccagcg gcgccgggaa gctgtactcg ccgatgacat cgcctgccag
    11761 gcaggtcagg ccgccgaggc ggaaggtgtg cgccagtttg ccgggctcat cactgcccaa
    11821 gatgtgcggg cggtagggca tctccagcgt gtccggcatg tgcgcggtgg cggaggtgtc
    11881 gaggatggcc gtgggaatgt ccgtggggac gatgtccatc accgtggcga tgagccagcc
    11941 tgtgttcagg gccacggcct cgccgggttc cagatagact tccttgcccc accgctcctt
    12001 gaagccgcgc accacgcgca cgagccgctc cacatcgtag tcgttgcgcg tgatgtgatg
    12061 gccgccgccc atgttcaccc atttcacttt ttccagcagc ctgggaaacc ggcgctcaac
    12121 cgccgcaagg gtgcgctcca ggacatcgga atcctgctcg cacagggcat gaaaatgcag
    12181 gccctccaag ccgtcgaggc tctcgttttc cagcgcgtcc gcgcgcgtgc cgaggcggca
    12241 gccgggcgag cagggatcat aaagactgac ctccacctcg ctgtgctccg gattcacgcg
    12301 gatgccgggg ctgggcgcgc gttttcctgc ggcgcgggcg gcgtcgagca tggggcggaa
    12361 tttccgccac tgcgtgggcg agttgaagct gatgtggtct gcaatgggga tgatctcccg
    12421 catctcagcc accttgaacg ctggcgcata gacatgcacc tcgcgtccga actcctcatg
    12481 cgccagcagc gcctcgtgga tgccgctggc gcagcagccg cgcaggaact ggcggacaag
    12541 tgggaaggcg gcgaacatgg agaagccctt caaggcaagc aggatgctcg cgcccgacgc
    12601 ctcctgcacg cgcgcgagct tctccaggtt cttttgcaaa agccccaggt ccacgacaaa
    12661 ggccggtgtc tccacctggg agagatcaaa ggccggggcg ggctgctcga agacatgcgg
    12721 gggtgtgaaa gacatgcgcc tgctcataga gagaccgcgc accgccgcca gtgaaaccta
    12781 tgccgcgaac agccgcgcct cacccgcgcg tgatgcgctg ctggagcagc acctcgaaga
    12841 aaagcaggcc cagcagcgcc agcagcagcg gctgccagaa ttcgctgccg aagcgccgcg
    12901 tgcggtccag tttgcgccag gcgtcgaggg actcgacgaa ttccgcgccg tgtctttcgg
    12961 cgatccggcg cacgttttcc gcgggcatgg gggtgatggc agactccgcc gtgtccaggt
    13021 tcaccgcgaa gaggcgggtt cgtccacccg cgacgagttg gtagatgccc ggcgtctcca
    13081 ggggtggagt cttgagcatc gtgttccctc cctcgactat gggtgtgagg gtgctcgtca
    13141 gtcccgcagg atcgcgcagg ctgtgctccg cgctggcaag ctccctgggc acggtcagtc
    13201 gcgtcggcgt gccggcggga atccaggctg tgctggtgct ctgagtggcg agatgcgcgg
    13261 ccagcctttg catgagcggc acataaaacg gctgcaaggg caggtttgac cactcagcgt
    13321 ccgccgtcgt ggccatggcg atgacgcgcc cgcttccgtg ctgcttttcc acgatgagtg
    13381 gaacgccttg atccagttgg agcaggggct ttgcctccgt gacggcgtcg agttcgaacc
    13441 aagactggaa ctcggcatct tgcagccttc cgccgcgggc gtcgttgaaa taaagcactg
    13501 ccggatgagt aaggcgttgc tgaaggatgc gcgccggcat cccttgcgcg cgcttgagcc
    13561 cggagatggc ggcggggaaa agcccctcgc cttttttgaa aaaatcgcgg ttgtaccact
    13621 ccagatcgca gtgcggcccg gcgaaaacga tgaggccgcc gcccgcgctc acaaacttgt
    13681 ccagatcggc catgcggttt ccttgcagcc tttccacatc tgcgaggatg accacttcct
    13741 tgccgcgcat gtcctcctca cggaagcggc gcgcgtccac cttggtcgcg cggatcaggt
    13801 ccttgagcga ggcgctggcg gactggtagg gcgccagcgc cagttccagg aaatccgccg
    13861 cgccacccag gggagtcgtg ctcggatcac catccacaag cagcacgttg agctggtcgc
    13921 gcacctgcac cacggagtaa aaggcgttgt cgtcggggaa gctgtcgccc tcgatgcgca
    13981 cggccagcga atgctcgccg gtggtttcga aggcgtgggt gaagttgacg atggactcgc
    14041 cctccgccgg cagcgacacg cgcgtgctgc gcaggcgcgc gccgtccgcc tccagatgca
    14101 cggcaacatc ctgccagggt tttttaccgt ggttgacaat gcgcgcgcgc aggcccgcag
    14161 gctggccctc cgccagcacc agagcggaga gttccgcgct ggcgatgctg aggttctccg
    14221 ccagatcgga ggagatgcgg taaaaggtca cctgtggcgc cggctcctgc ttgagcagcg
    14281 cgtcgagcgc cggcagcgcg gcgccatccg cggcgccctg ccagtcggcg gcctgaaaat
    14341 cggagatgac cgccagctcg cgcgcggcgt tttgggactt cgccagcgcc gccgcggcag
    14401 cctgcagcgc gtcgttcacc cgcaccggcc ctgcgaggcg gctgctctcc gcgagctgtc
    14461 ccggcagttc ttccagtgct gtcacgggat cctcgaaaag tgtccgcgca ttgccccccg
    14521 ccagtacgac ctgcgcgtcc gagccgccgg gcaggtcctg cagaatgccc gcgatgtcct
    14581 cacgcgcgcg ctccccgggc gtgccgcctg atgagggcgc gcgcatggaa aatgaatcat
    14641 ccagcagcat aaccagcgag gtctggccga ggccgaagga gcgcagcgcg gtcagcacag
    14701 gacgcgcgag gcaaagcgcg agcacgatgg gaatggagac gcgcacggcc agcagcagcc
    14761 attgctcgat tttcatgcgc cgcttccgct ggcggaccac ctcgtgcagc aggtgcatgg
    14821 cgccccagcg cacagtgatg acgcgccgct tgttcagcag atgaatgatg agcgggatca
    14881 ggaaggccga tgcgcccgcg agaaggatgg cgttgagaaa ggtcaagctg ggggaactag
    14941 catggacgct tgcatgcggc agcttttttc ttgggattgc gcgcgggatc gcggactttc
    15001 caccgcgcgg caaaatgagc ctttgcccgg tgtgctccgc cgtttatgga agccgccttc
    15061 cattttccgc atgaacctcc gcgtcatctc ggcccttgtg ctgcgttaca tctttcttta
    15121 cacacgcaat cccatccgcc tggtggagat gattttctgg ccgttggtgg acctgctcgt
    15181 ctggggaaac ctcaccctct ttctccaaag gaacacgacc accgagttcg gcggcttcat
    15241 cctcttcctg ctgggggcca tgatcttgtg ggacattctc ttccgcgccc agcagggcgt
    15301 ggccatctcc ttcctggagg atgtgtggac gcggaacctg ctcaatgtct tcgtggctcc
    15361 ggtgcgcacc accgagtatc tggcggccac ctttgccgtg ggaatgctgc gcatttttgt
    15421 gaccgtggcg gtgctgggac tggtggcctg ggcgggctac cggttcgaca ttttcattct
    15481 ggactggtgg ctgctgccct tttttctgaa cctcatggtc ttcggctggt ccctgggcat
    15541 gatctccacg gcgctcattc tgcgctgggg ccaggcggcg gagtcgctgg cgtgggcggt
    15601 gccgtttttc atccagccca tcgtggcggt tttctatccg gtggcggaca tgcccgcttg
    15661 gtcgcagcct ttcgcctggt gtttccccgc cacttatgtc ttcgagggca tgcgccaggt
    15721 catgaacggc ggtgtcatgg acatggggct tctcgtcaaa gccgtgctgc tgaacgcggg
    15781 ctacctcgcc gccgccgggg cgctgttctt ccacatcctg cgcgtgactc gaaagcgcgg
    15841 cctgctcacc aagtttgcca cgcagtaaac gcgaaaaacc cccggcggcc atgccgccgg
    15901 gggttggcgc ggaagcctcc gctcactgcg ccgtcaccac gattctttgg ttgttgcgcc
    15961 ccacattgga gatgaactgc gcaaagggat ggaagatgtc caccgcgcgg gtgcccaggc
    16021 ggaagctcac ctggatgctg ttgtcagccc acgccgtctc ggtgtagcgt ccggtttcca
    16081 ctgtgacggg cttgctcccg gtggagtcaa taatctggta gctgccattg gcgttgaagc
    16141 gcaccgtgcg ctggtagaga ttgccgttgt tgtccgccag gacgtcggac ttgtagtccc
    16201 tggaggcgag gaagttggtc atgagttgcg ccagggtcac acgggggatg gtcagtgtcg
    16261 tcgtgcggcc cgcggtcagg aacgcgttga acgtgaagtt gaacagcggc tcgccggggt
    16321 tgatgccgat ctccaccacc acggtcttgc ggccaggagt gtggatgcct tcctgcaatc
    16381 tgccaggagg gatgccattg ccctgcgcga actgctccac gccgttcaca cgcaggctga
    16441 tggcgttgta ctggctgttg ttctgcatgt tgagccggct ctgctgcgcg ggcagcgggt
    16501 tgttcaccgt gaaggaaaag agcgtgctgg tcacatactc ctgctgcacc aggcggatcc
    16561 tgccggagac ggcgctggcc ggcactgtca cgcgcaggct gttggtgctc acgaaggtga
    16621 aggcggcgtt cacaaactgg cctggagccg cggtgggaaa ctggatctgg tagggcgcgc
    16681 ggcccgtcca gagattgccg tccttgtcgc gtcgcagccc gtctccgagc agggtgatga
    16741 cggtgcgtgg tgccgaggcg ttcgggctgg ccaggatgac cctggggcgc gtgctttcga
    16801 cgacgttgcc aaaggccgat gcggtgcgcg gcgttgacag ggctgcggcg gcgcaaaggg
    16861 cggcggcgca gaggagggtg gtggacttgg tgacgagtgt tttcatgacg gtgtgtggtg
    16921 tgagtgtttt tggggccggg atggccgcct ttcacaccgc agatgcccgc ccgcccgcga
    16981 acttgcgcag attcatgaaa aaagtttcag ccccgcctcg gaggccgtct caaacacctc
    17041 attccgggcg cttcgcatct ggcgtttcga attccaggct gtgaattttt cccgcctcat
    17101 cgggagtgcc gagcactttc cacacgcacc agccgaagag gctgacgaca gttcctactg
    17161 agcagagcat gacgatgagg ccggcggtgg tcatgggatg atgagggctg tggattgagg
    17221 gttccggggc gggggagtca aacgtcgtcc ttgtcgagac cctgctcggc gcgctggaaa
    17281 gcgcgcgcgc gcgaggtcat gagggagaag aacacggcga tcacgatgac caggccgacg
    17341 ctgagctgcg cgggcaggct ggccttttcg ccaaagagat cggtgacgta atacgagacg
    17401 ccacccgccg cgcccgaact cagatccatg ccaaggatct cctgcatgaa ccagagcgca
    17461 aagatgccga gcaggaaggc cgggcagatc cacttgatga cgggccggaa tatccacggc
    17521 acctggatgg ccgccccctg gtgcagctcg tcgaagccgc ggtcaatgcc ccacacccag
    17581 ccgaagacga tgatctggat ggttgccagc atgaagatga ggaaggtgcc cacccagaag
    17641 tccagagtgt ccagcgcctt caggtcgccg gtgaagtaga tgacgaaacc cgcgccaaag
    17701 caggtgagca gtccgagcag ggtgacggac accttgcgcc cgactccgag cgcctcctcg
    17761 acaaaggcga tgccgggctg gagcatcgag atggagctgg tgatggcggc cagaaagagc
    17821 atgaagaaaa acgcgccgcc gaagaaatcc cccgccggca tcttcgcgaa caccagcggc
    17881 agaactttga agccgaggcc cacgcctgcc tgtcccgcca cacccgccac gccaagaaaa
    17941 gccaccgccg ccggcacagt gatcaggccg ccgaggccca cctcgcaaaa ctcattggcg
    18001 ctgctggcgg tgaggccgct gagcaccacg tcatcgcttt ttttcatgta gctggcatag
    18061 acgatgatga caccgaagcc gacggacagg ctgaagaaaa tctgcccggc ggccgcgagc
    18121 cagaggcgcg gattgcgcag ttgatccagc acgcccgcct cccttagcgc cagggcgcct
    18181 ccgctggcgg ccacctccgt ctttgccgcc tcgatggccg ccgcgcccac cacctcgcgc
    18241 ggtttgtcct tcgcgtccgg cgcggtgtgg accaccatca ctttggttgg attccacagg
    18301 tagcccaggc cgttgcccac gctgtcattc ggacgggcag cgtccggcgc gccgagggtc
    18361 agcacgcgcg ccagcaaaat cagcgccagg aggatgagca tgggcatggc gatcttgcac
    18421 attttctcga tgccgccgga gatgcccctg taaattagcc agaaattgag aatgaacacg
    18481 ccaagcagcc agggcagcac cttgccggag tcaaaactca tcgccgagcc gtccgccgcc
    18541 gcgccggtga actcggcgaa gcgcgccacg gtctgaccgg agttttccag ccgcaggccg
    18601 ccgctccaaa agttcacggc atagccgatg caccaggact cgatgaccac ataatacatg
    18661 tagatgacca ccggcacgat gacgccgatg atgcccgcat acttcgccca gggtcggcgc
    18721 acgatggcgg cgaacgcgcc ggggcaggaa ttgtagccgc gcccgcccgc gtgacggccc
    18781 atggcccact ccgcccagcc gatgggcagg ccgatgatca gaaagctgat ggcataggcc
    18841 agcatgaagg ccccgccgcc atactgcgcg gccagccccg ggaagcgcag gaaattcccc
    18901 agtcccaccg cgctgccggc cacggccatg atgactccga gacgtgatcc ccaggcttct
    18961 tttttcgatg acataaaatc gaaattagaa aaccccgtgc caggacacaa gcctcaatcc
    19021 accggaatgc gtcttctttc ttcagaaccg cttccctggc ccgccccgct gacagtcaga
    19081 atgcgccgcc gtccggcgcg ccgcccttcc ttgacccgcc gcgcaaacag ggttaggcac
    19141 tccgcccccc cagccgacac cccctgatgt ccatcgcccg cacacgcaac ttctccatca
    19201 tcgcccacat tgatcacggg aagaccacgc tctcagaccg gctccttgag ttcaccaaga
    19261 ccatcaccga gcgtcagcag caggatcagc tcctggactc catggacctg gagcgcgagc
    19321 gcggcatcac catcaaggcg catcccgtgt gcatgaacta caaggccaaa gacggccatc
    19381 tctacaagct caacctgctc gacacccccg gccatgtgga cttcagctac gaggtcagcc
    19441 gctccctggc cgcctgcgag ggcgccctcc tgctcgtgga cgcctcccag ggtgtcgagg
    19501 cgcagacagt ggccaatctc aacctcgccc ttcagcaaaa cctccacgtc atcccggtca
    19561 tcaacaagat tgacctgccc agcgccgatg tggacaaggt caaacgccag cttgaggaca
    19621 tcctccagct tcccgccgac gaggccgtgt ccgcctccgc caaaatgggc attggcattg
    19681 aggacattct ggaagccgtc gtggcgttta ttcctccgcc caaggagcct ggtgacggct
    19741 acctgcgcgc ctccgtcttc gactccgtgt tcgacgccta ccgcggtgtt gtgagttatg
    19801 tgcgcgtcgt ttccggcacc atccagcggg ggcagaaggt gcgcatgatg tccaccggca
    19861 acgactatga gatcaaggac gtgggcatct tccgtccgaa gatgacgagt tgtgaaaagc
    19921 ttgaggcggg cgatgtcggc tacctcattg ccaacgtcaa atccaccgcc gatgtcaaga
    19981 tcggcgacac cctcaccgag agccggcggc ccgcgccaga gccgctgccc ggtttcaaag
    20041 aaatccaccc cctcgtcttc agcggcatct accccgtgag caccgacgac ttcgaagcgc
    20101 tcaagctggc tgtgggcaag ctgcaaatca atgatgccgc attcaccttc atggccgaaa
    20161 gctccgccgc actcggcttc ggcttccgct gcggcttcct cggcctgctg cacatggaga
    20221 tcgtgcagga gcgcctgcgc cgtgagttca acatggacgt catctccacc tatccttcgg
    20281 tcatttacga ggtggtgaag accgacggca cagagctgct cgtggacaac cccagcttcc
    20341 tgcccgccgc caacgagatc gaggaaatcc gcgagcccat cgtcaaagtg tacatcatga
    20401 tccccaacga gcacatcggc gacatcatgc agctcgtcat ggacaagcgc ggccaggtca
    20461 acaacaccga gaccctcgac gacaagcgcg tcatgctcca caccgtgctg cccctcaacg
    20521 agatcctcgt ggacttcaat gacaagctca agtccctcac ccgcggctac ggcagcatgg
    20581 actacgagca cgccggctac caggcggccg accttgtgaa aatggacatg ctcatcgcca
    20641 gcgagcccgt ggacgccttc tcctgcatcg tccaccgcag caaggccgaa agccgcggcc
    20701 gccagctcgc cgcgaagctc aaggaggtca tcccccagca gctcttcgtc gtcgccatcc
    20761 aggccgccat cggcggcaaa gtcatcgccc gcgaaagcat cagcgccctg cgcaaggacg
    20821 tgaccgccaa atgttatggc ggcgacatca cccgcaagcg caagcttctg gagaagcaga
    20881 aggagggcaa aaagaagatg aaagccatcg gccgcgtcaa catcccccag gaagccttca
    20941 tcgaagtcct caagaccaac tgagccgtga ccggcgcgca agattcgccg cgcgagcgac
    21001 ccgaggaggc ggacgcccgc tgtagaatcc ttccgcatcg tcacgtctga ggcttttgca
    21061 ggcgccaggg cggcgcgcgg tcatccgcag gtcaagcatg ggcatggatt gggctttgcc
    21121 cggagggcgc gcggcattac atctgcgcct ttccacgccc gaccatgaat gaagaccgct
    21181 ccctctcgcg caccgcctgg atggtggtgg ccctgctgtt ccccgtggcg ctgctgaact
    21241 atctggaccg gcagatgatc gccgccatga aggtgtcggt gatgggggac atccaggaca
    21301 tcggcagcga tgagaactgg gggcacatgc tggcctcgtt caagtgggtg tacgcggttt
    21361 tcagtcctct tggcggttat attgcggatc gtttcagccg caagctgacg atttgcagca
    21421 gtctgttcgt gtggtccctg atcacctggc tcacgggcca tgtcagcagc tttcaggagc
    21481 tgtactgggc gcgcacagcg atgggaatca gcgaggcgtt ttacatcccg gcggcgctgg
    21541 cgctgatcgc tgattaccac acggggggca cgcgctcgcg ggccgtgggt gtgcatcaaa
    21601 tgggcattta ttgcgggcag atgctgggag gctttgccgg attttccgac aggctggagg
    21661 gtggctggcg ctctcttttt gatgtcaccg ggctgaccgg catcctttat gccattcctc
    21721 tgttgttcct gctgcgtgac gctccctcca agccccgtcc cgatgtcaat ggtggtccgg
    21781 cggcccaaac ccgtgccggc acggggattg tggagctgct cacgaaccgc aacttcatcc
    21841 tgctcgtggc ctatttcacc ctgcccgcca tcgccgcatg ggtggtgcgg gactggatgc
    21901 cggcgattct gcaaaaggct ttcgacatca gccagggcaa ggcgggtgtg tcggccacgc
    21961 tttactggca ggcggcggcg ctgctctccg cgatttttgg cggctggctg gctgaccggt
    22021 ggatgcgcaa gagcatccgc gggcgcatcc atgtcagtgc gatcggcatg atgctgattg
    22081 ttcctgccat gtttggcgtg ggcaacgcgc ctgctttgaa ctcgttcgcg ctggccatcg
    22141 tcgcgctggt cctgtttggc gtcggctggg gtttctttga ctgcaacaac atgcctattc
    22201 tgagccagat cgtgcgtccg gagctgcgcg ccacaggcta cgggatcatg aatttcgtca
    22261 gcatgatgtg cggcggcgtc gcggactggg gctttggcgc gatgacggac cgtggcgtgc
    22321 cgttgaatgt gatcttcggg gtgtttgccc tggtttgcgt ggcttcgttg ttcatcgtct
    22381 tgctgatccg cccgcgtgag acgcctggcg gacagggctg aaaatggagg gtgcttttcc
    22441 cgttcgacaa tccgccttcc cgatttacta acgcccatga cccaactgct cgcccccgcc
    22501 atcgcctccc gaattgacga actctgcgcc gccatcgccg cggacccgga cgtgcaaaaa
    22561 gcgcgggacc aggcggaggc gtttctcgcc gatgaggacg ccgtgggcct ctaccgggag
    22621 gtgatgaacc tgggccgcag cctggagcag cgccatcgcg gcgggcagaa gctcgacgcg
    22681 caggaggtga gccgctttca agacctgcag gaccaggccg acgcgcacga gggcatccag
    22741 gcttttgtgg ccgcgcagga tgtgctgcag gaggtggcaa accgggtgaa cggctttgtc
    22801 accaagacgc tggaaaaggg ccgcgtgccc acgcaggagg aagtcttcgg ccagggcggc
    22861 tgcggcgagg gctgcggctg ccaccattga cgcgcatggc ccggacccgc ctgccgcgcc
    22921 tgctg
//
LOCUS       DAOMYJ010000068        22623 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Prosthecobacter sp. isolate AMR_MDS_5629
            NODE_823_length_22623_cov_21.821030, whole genome shotgun sequence.
ACCESSION   DAOMYJ010000068 DAOMYJ010000000
VERSION     DAOMYJ010000068.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867850
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prosthecobacter sp. (wastewater metagenome)
  ORGANISM  Prosthecobacter sp.
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Verrucomicrobiaceae; Prosthecobacter.
REFERENCE   1  (bases 1 to 22623)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 22623)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.30
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:34:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,742
            CDSs (total)                      :: 4,684
            Genes (coding)                    :: 4,676
            CDSs (with protein)               :: 4,676
            Genes (RNA)                       :: 58
            tRNAs                             :: 55
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22623
                     /organism="Prosthecobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_823_length_22623_cov_21.821030"
                     /isolate="AMR_MDS_5629"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1965333"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..202)
                     /locus_tag="PLP58_09190"
     CDS             complement(<1..202)
                     /locus_tag="PLP58_09190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009963832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite reductase subunit alpha"
                     /protein_id="HRJ08730.1"
                     /translation="MQITPLIPESAPFTAEQRAWLNGFIAGVLKLGGSPGASSAHAQA
                     KTSLLIAFGSQSGNAESLAKRLA"
     gene            complement(215..1570)
                     /locus_tag="PLP58_09195"
     CDS             complement(215..1570)
                     /locus_tag="PLP58_09195"
                     /inference="COORDINATES: protein motif:HMM:NF024765.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alginate export family protein"
                     /protein_id="HRJ08731.1"
                     /translation="MASLTHAGSPQTAPLAAPPPPANGFTPITFFDGKLTVDVQEKMR
                     FEARENNFDFNSAVNGPQDASWLLQRFRLGLGYSFTPWFKIYVQGQDVREIGGSRPNE
                     IGVFGAEGDDVFDVLKAFVQLGDAKAGLSATVGRQFLSYGDQRLVGPLEWLNQARTFD
                     AVKLRYASESWAFDLFTSSPVTFKDHEWNKSQIFDAQSPDDTIFSGAYLATKWVPINT
                     TTDFYIFHKTDNGNGAFGPALGDTSFVTMGTLWKGDPKKLGGWDYETEMAFQTGDANG
                     RNLRAFAGHWGIGYNWLNHPWKPRIGLQYNWSPGDSDATDGDLGTFQNLYPTNHLFYG
                     YMDTTGWINMHNPQLNISVMPTPKLKLMLDYHLYWNDTNEDAWRRVNGVTAVRAVNAV
                     SREASNFRGSELDLTASYKLNAHVGLMAGYSVFFAGHYLADTGASNNAHFGYVQLQID
                     F"
     gene            complement(1633..2493)
                     /locus_tag="PLP58_09200"
     CDS             complement(1633..2493)
                     /locus_tag="PLP58_09200"
                     /EC_number="7.6.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009963823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRJ08732.1"
                     /translation="MLELDRLWKIYDTPKGPATIVKNFNLKLKEGEFATLIGHSGCGK
                     STVLMMVAGLSDLSKGNMILAGKEAVGPGPDRGIVFQSPCLLPWMSAFENVMLGVNQV
                     YFTATRAERRQMAEYYLSIVGLADAMHKFPGELSQGMRQRVGIARAFALQPKMLLLDE
                     PFGMLDSLTRYELQEVLLDLWRRNRITTLMVTHDVDEAIFLSDRVVMMTDGPEAEVGD
                     ILKIPFERPRNRKEIMEDPRYYELREHLITFLNDKSHIRPSREPEFNPSPDMAAEMAA
                     HGLLFPTAAA"
     gene            complement(2511..3431)
                     /locus_tag="PLP58_09205"
     CDS             complement(2511..3431)
                     /locus_tag="PLP58_09205"
                     /EC_number="7.6.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007415864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRJ08733.1"
                     /translation="MPLIEIKGASKGYGHNGSRSEVLKNINLSVEEGEFVVIVGYSGS
                     GKSTLINLISGLVKPDAGEALIEGRKITEPGPDRGLVFQNYSLLPWLTVEENIALAVD
                     EVFKEWTPKQRKEHVAKHIAMVKLSHAAHKLPKELSGGMRQRVSVARTLAMNPKVLLM
                     DEPLSALDALTRATLQDEISEIWQSNKTTVIWITNDPDEAILVADKVIPLLPTAPATL
                     GEPMLIDLERPRDRRSINHDPKFKKLRTQLITTLLDAKGRTKSKATMKLTLPDILPED
                     ITNKNSLPFLNRGGPKRRNADKREEVEVTA"
     gene            complement(3451..4338)
                     /gene="ntrB"
                     /locus_tag="PLP58_09210"
     CDS             complement(3451..4338)
                     /gene="ntrB"
                     /locus_tag="PLP58_09210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009963820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015414 - ABC-type nitrate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015706 - nitrate transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate ABC transporter permease"
                     /protein_id="HRJ08734.1"
                     /translation="MHPLIQKYKLDSIFLPLVAIVITCVFWYAIAGKSVVTEKVDDFG
                     DKVKITKREGLSADLPTPGETWTASKPYIVEPFAKRGELDQGILRFTWMSLVLVAQGY
                     LIALVIGTPIGFFLGLSKPFTKAFDPIIQVLRPVSPLAWLPLGMILFSGVKILDGDGR
                     TTFGASDAAALFTIAICAMWPTVMNTAVGVRAVPVDYLNVAKVLKLSKWKTLWKVLVP
                     ATLPYMFTGFRLSLGIAWLVIVAVEMLTGRPGVGGFLWQQYNANSYAHIILCILTIGV
                     IGYVLDRLMSVVEGRFKTA"
     gene            complement(4404..5678)
                     /locus_tag="PLP58_09215"
     CDS             complement(4404..5678)
                     /locus_tag="PLP58_09215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007415866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CmpA/NrtA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HRJ08735.1"
                     /translation="MNTATPLTRRSILRQAGLGALLSGLPSGWVGSAYADDSPEVADL
                     KFGIIALTDCSSIVVAHEKGLFKKYGINSTVSKGASWAAIRDSLSNGDIQATHMLIGM
                     PIASTMGLGGAPKKPMIIPWLINRNGQAISLSNAYKGKVTDDPKALKPFVDDAKAKGA
                     PLTFAMTFPPGTHAMWMRYYLAAGGINPGDANGAGADVSLITVPPPQMVANMKVGKMD
                     GFCVGEPWNAKTIADDIGFTSITTQAMWKDHPEKVCAFTEEFAAKNPKTVKAVIKALH
                     EASVWLDKMENREEQANIVSAATYINCPPETILGRLQGKYLMGDGRKFKDPNYMIFSD
                     RNCNYPQAKYCKWWLTQLRRWGFVEGAPDYEGVTKQVMRSDIYEEAMKEIGYAHGGVD
                     TSAETLFDGVTFDPKADLEAYAASFAVKTIKG"
     gene            complement(5675..6787)
                     /locus_tag="PLP58_09220"
     CDS             complement(5675..6787)
                     /locus_tag="PLP58_09220"
                     /inference="COORDINATES: protein motif:HMM:NF024772.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CmpA/NrtA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HRJ08736.1"
                     /translation="MPKHASSPAPALTGVIQPVRIGFIPLADCAPLLVAGSRDLFRKH
                     GVRVELSCEVGWATIREKLLYGQVDAAHAIAGLALAMRMGISSPPSPVVAPFVFNLHG
                     DAITLSRDLWNRGVRDAASLKKLIRSTTSRRLTFGMVSRYASHHFLLRRWLISGGIHP
                     GQDVRIVALPPTQMAANLAAGLIDGYCVGEPWNSVAVEKGAGWVVATSEQLAPGHPEK
                     ALIMGGAFITRHRDQAQAVINALRESCAFCDAPENRPEVVKVLAGSGFFNGCESMLKK
                     SLIGPFDPGTGQNWDASSFHIFHRREANEPTSERGRWLLDEFIAHGLLMPAQRADAAA
                     SLRDCWTSGALYFPEAPAAAPKPVSKPKKRKPLAHA"
     gene            6881..7783
                     /locus_tag="PLP58_09225"
     CDS             6881..7783
                     /locus_tag="PLP58_09225"
                     /inference="COORDINATES: protein motif:HMM:NF015431.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HRJ08737.1"
                     /translation="MEQPLDTRQLRAFISLARSGSFTQAGRELHLTQSAISHAIKALE
                     NDLGCQLFHRQGKSVHLTHHGRELLPHAESILQEMGQARAALGALDRTPRGRLSIGCT
                     PAASQFILPTVFREFKESFPQYEIRVVPGETPQTIERLLNNEVDLAVTLRPPDAGRLE
                     CQPVLEDELEFLVSPLHPWTTKPPRVKDAAAETFIVSSRSSLNFQMIQEFFLRQGVRL
                     NHFIELGSSEAAKELAKLGLGIAIAARWIARAEIEAGQLVPVPLPKSKLRRRWVVAWL
                     KGRPLNLAERTFTGLCEEVGRRML"
     gene            complement(7798..8850)
                     /locus_tag="PLP58_09230"
     CDS             complement(7798..8850)
                     /locus_tag="PLP58_09230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ08738.1"
                     /translation="MKHALLILPLAALVPGAFQLIAQVAAKKSGIEARLEQALRMFPD
                     ADTDKDGALSINEALAYVEKHPEARDKFMARAGSGKSSSKPASFAPGAEGTKVFVCAH
                     SYMIYTADWLPQIAQAAGVPHLKAGQQMIGGSRVQQHWDLPDERNQAKKALQEGIVDV
                     LTLSPNLLMPDEGIDRFTRLGLEKNPELRVLVQASWAPRDGKAGAGFANAMRDSVTAE
                     EIDSMLEAHNGWLSLLEKQVTGLNTELGRPSVRIIPASAALHSLRRHVAEGRAPGVSK
                     QSELFRDDHGHPSDITALLVSYCHFACIYQRTPVGLPVPEKLSARPQSSDLVRLLQQI
                     AWDAVTSHPMSGVKKA"
     gene            complement(8937..10730)
                     /locus_tag="PLP58_09235"
     CDS             complement(8937..10730)
                     /locus_tag="PLP58_09235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ08739.1"
                     /translation="MKCLTLCLLTALFCARAVAQDYFRHDSRALTQASKAVLITDLSR
                     MQPQAALITGRRQKGKWKMLPFETAEMKGTALSVYSATNPPRVSLPLLEKGWHSVHVG
                     LATTSGGFNIGGNGIKAKLSDEPVFRRLANNLALLPNRRAVIQECFVSVAELNGQSLE
                     IAPMPGLPATVCYVKLVPMTLEEVAASRERSKHRTSIATFDGHSWIWPFKPTTAEELA
                     EEFRGYEDTDIGKWWFQVTGGDLVCYPSKVGTYPGEGTVDFPTGAYAAYTKSLETLFK
                     AGVNPLKVARDAAKAQGAEFHVMLRPGGWAGSMPYEETFNSRFYYEHPEWRCLDRDGT
                     PTFYMSYAVPEVRRHLIELLRETLELQPEGVGFLFNRGMPLMLWEEAFCVRFKEMHNA
                     DARSVEDDDPRIQSTRAAVMTGFMREVRALLDETAARQGRKERYKISLGTFAREADNQ
                     RWGLDLPNWIQLGLVDDIAVTWFAYHTSFEKTPGQIDMDYYRRITQGTSARAWPMVIA
                     WKTGKPKELCQKTAAWLADGAPGIAIWDPQVMKGWPEGSPGNVFDVLGRLGHKDDLLR
                     WSKNGVPSPLSVPLTRLDDNHFSRWFPNTGF"
     gene            complement(10797..11801)
                     /gene="rfaD"
                     /locus_tag="PLP58_09240"
     CDS             complement(10797..11801)
                     /gene="rfaD"
                     /locus_tag="PLP58_09240"
                     /EC_number="5.1.3.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009962075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008712 - ADP-glyceromanno-heptose
                     6-epimerase activity [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-glyceromanno-heptose 6-epimerase"
                     /protein_id="HRJ08740.1"
                     /translation="MPIQPQSRILVTGGAGFIGSALVWELNRRGCENIIVSDRLCTDE
                     KWRNLAPLRFHDYLDGGDLERLARTDPSALGKFDLVLHLGACSATTEKDADYLMRNNY
                     ELTRVLCGWALGQDARFVYASSAATYGDGAQGMDDRMDDPHRLRPLNMYGYSKHLFDL
                     HARRTGMLGRIAGLKYFNVYGPNEDHKADMRSVVHKAHRQILDTGRVQLFKSHRPDYQ
                     DGCQMRDFLYVKDAVKMTLHLAETTTANGVFNLGSGKAHTWVELVGAIFAAMGREPVV
                     EFIEMPAHLRDKYQYHTCADISKLRASGYEEPLFTLAEAVRDYVRGYLETDKRLGDEV
                     "
     gene            complement(12127..12525)
                     /locus_tag="PLP58_09245"
     CDS             complement(12127..12525)
                     /locus_tag="PLP58_09245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ08741.1"
                     /translation="MKAIIEHLIVAVIAVGMCVVGFWLIGWHNGTGDMGILPYEPGWT
                     IQKFTVSILTWIWLYRRGRNLAWFVLPILGLVSPFIGAVLFIYPYFFWPWVVLCHHAI
                     VIFPTGLVCGLLVSVATLPFRPPQVVCGNA"
     gene            complement(12533..13909)
                     /gene="lpdA"
                     /locus_tag="PLP58_09250"
     CDS             complement(12533..13909)
                     /gene="lpdA"
                     /locus_tag="PLP58_09250"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012420722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004148 - dihydrolipoyl dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA];
                     GO_process: GO:0051068 - dihydrolipoamide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HRJ08742.1"
                     /translation="MNYDLIVIGGGPAGYVGAIRAAQLGKKVACVENDRAGGTCLNWG
                     CIPTKALLKNAELFHTLTHRAAEFGLKIGSIEYDWSKVIGRSRGVSDKLNKGIEFLFK
                     KNKVDYLKGTASIPAAGKVEVTAADGRKETHDAANILIATGAKSRPMPGFSFNGTTVI
                     GSKEAMTLEKQPKSVIVIGAGAIGIEFAYFFNAYGTKVTVVEMMPDILPVEDTEVSKT
                     LEKSLTKAGIRILTNTKVTGTSEDGKGVKITVEGAANETLEAEVALVAIGVAPVLPGG
                     IELKLSDRGWLETNERYETSVKGIYGAGDIIGPPWLAHVASYEAVQCVEGLFTKHKPK
                     KVGVFPGCTYCHPQVASIGLTERAAKEKGLKFKVGKFPYVASGKALAAAEPEGFVKIL
                     YGEPHGEIIGAHMIGSESTEMIAEIGLAMNLEATWDEIEATIHAHPTLSEMVKEATEV
                     AKGHPIHV"
     gene            complement(14024..15565)
                     /gene="zwf"
                     /locus_tag="PLP58_09255"
     CDS             complement(14024..15565)
                     /gene="zwf"
                     /locus_tag="PLP58_09255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009958582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004345 - glucose-6-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HRJ08743.1"
                     /translation="MPDLENPFEETLLRRNRAEPCTVVIFGATGDLTHRKLIPALYNI
                     AACGDLPPQFKVVGFARRDKTDELFRSELEAGNRKNSRQGHNDELWANFSHSIHYHRS
                     EFEDVEGYRALARLLDQFDEERGAPANRLFYLASAPEYFKPILELIREAGLNQGAGGC
                     WARVVCEKPFGKDLQSARTLNEVVAGTFEEADTYRIDHYLGKETAQNIMVLRFANALF
                     EPNWNSRYIDHIQITCAENLGMEGGRGGYYDTAGALRDMVQNHLFQLLTLVAMEPPTD
                     LSADSVRDEKVKVIRALRPLVGPADVGANVIRAQYTSGSVDGLPRVGYREEDRVNPES
                     KTEAYVALRLHVNTWRWQGVPFYIRVGKQLPKKATEISVHFKKPPQVPFATAQLPGNS
                     ENTLVIRIQPDEGIALRILCKQPGQALSMQQVKMDFRYSSSFGRASPEAYERLLLDAM
                     AGDATLFARRDEVENAWKFIDEIENAWHKTSTPPPMCEYPAGSWGPKEADQLLAQDGR
                     VWRLL"
     gene            15743..16840
                     /locus_tag="PLP58_09260"
     CDS             15743..16840
                     /locus_tag="PLP58_09260"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009965026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HRJ08744.1"
                     /translation="MPATTILPTDQPPLLHAAVDQAVKLLQSGEVVALPTETVYGLAA
                     DALNPAAVAKIFEAKERPAFDPLIVHLPDKKHLDTVVEVPPEVQKLVIQLIERFWPGP
                     LTLVLPKKPCVPGLVTAGLPSVAVRVSANPIFKRVAQALGRPLAAPSANRFGSISPTS
                     ASAVAAELDGRIPLILDGGACMHGLESTIIKVEAASPKNLITILRPGPITAEDLKVYG
                     KVMRVTRSVVDEATEAPGQLASHYAPRTPLRLLAKPSDFTPEPGKRYALLSYRGDEND
                     GFLGLADWLQVMILSPGKGKLPEAAVRFFYALRELDKLGADEIIAEPMHEHGMGAAMM
                     DKLRRAAVKAGRPSRLPESQSAAGTVAPPSA"
     gene            16837..18510
                     /gene="murJ"
                     /locus_tag="PLP58_09265"
     CDS             16837..18510
                     /gene="murJ"
                     /locus_tag="PLP58_09265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009965025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0034204 - lipid translocation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein biosynthesis integral membrane protein
                     MurJ"
                     /protein_id="HRJ08745.1"
                     /translation="MSDNAPSPDQPRRPADSKSATGTVAQPSQARSTSVVSIAILCSR
                     VLGLVRDQLLNGLFGAHLTGIFTAAFRTPNMLRDLFAEGALSTAFVTTFSKKIKTEGD
                     EAAWLLGRKMLALSICCMSMVSLAGIALAPWLVRLLNPGWDDPRSLAICTELVRIMYP
                     FITLVSVTALVMGMLNSKKVFFIPALASAFFNLGCIVGGVVLAWFMDPGFRDGAITEK
                     GLQAFAIGTLVGGLMQLAIQIPSLRRAGFRFGLDFKWRDKGVGEVLQLMWPSLIAAGG
                     TQIGVLLNSIFASFTPGKESAVAWLANAQRLQQLPLGLFGVAVATVTLPMLARLATEG
                     ITPAFRGALAKGLRLVLFLTLPSAIGLSLLAEEIVSILFQYGRFTAADVAATAGPLQA
                     YAFGLVFYSAIKVLQPAFYTIGRRFVPMQVSLGIIVFTASVNAFTVFVLEWDHTALAW
                     ATAAGLMLNFATLYLCMKKFANGLETRALLGALVRLAAGVACMALVCLAAKATLLSGW
                     AGMILLNKAAALGGTVTVAAAVYFYVTQKLRVEEAGEFTGILTRRFRRK"
     gene            18514..19110
                     /locus_tag="PLP58_09270"
     CDS             18514..19110
                     /locus_tag="PLP58_09270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ08746.1"
                     /translation="MKNQRIWITTATLLALIWGALAVVMRQTDPLVSWPQKVLDLAGA
                     APWHDGKTASESQRRLHLHEVITRLNQLDFQQRRALREDGQDILDKFFASLTEEEQKE
                     YVDRTVEPYFETISKGIKLLPEDERKRMVARMRGDLRNLRANSADGDRLSDQDREFFE
                     FMVAEDPVLFLREAPVKMKMELAPVIEDMQSRLQGFRR"
     gene            19117..20634
                     /locus_tag="PLP58_09275"
     CDS             19117..20634
                     /locus_tag="PLP58_09275"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase"
                     /protein_id="HRJ08747.1"
                     /translation="MTHPRAARVEMRMPIRLILSLALLSAPVLAAKPPNIVWIVADDM
                     SPDTAAYGMTGVSTPHLDRLAAEGARFTRAYASAPVCSSSRSAFILGVMQTTTGLHAH
                     DVENPQPLPAPWRHLPGLLRDAGWHVTNAPAPGTERGGRAVKKAKTHYNFAHDPAGMY
                     DGDDWRKRRPGQPFFAQFQIVEPHRPFPIPEEYDDALMARMSIPPNYPDHPLVRRDWY
                     AYLRSVEVVDKRVGDILQQLAGEGVLDDTLVIFFADHGRPMPWGKQWLTIEGLRVPLL
                     MRGPGVEAGRVEERLASLIDLAPTALQLAGLPLPEWMQGRPLLGAPFPERERIFAARD
                     RCGDAQDHIRAVITADHLFARHFHPGLSFMNWSGYKEASYPAMPLLRELAAAGSLTEM
                     QARYLKPDRPPLELHDLKSDPLGVTGAALSGDNARVLARLSGELDQWIKDSGDRGGLP
                     DPATEPGMEEIRKSKRQDYQRTWERRGLGPEPSDAERVSWWLRQYGLAEPSPPPR"
     gene            complement(20814..21458)
                     /locus_tag="PLP58_09280"
     CDS             complement(20814..21458)
                     /locus_tag="PLP58_09280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ08748.1"
                     /translation="MKKPALFLLALAFSHLHAGEIQRATLNADDWQPGDPPKEVFVVD
                     GKIQITAKDGNNAITVLPEAVTDASAQLGSSAAGESSIQARVFSARRARSNPRFGISV
                     HGMSGHRLILNPAKRQLELHKNDEILATVPHSWTSEAWVWLKLEARRGEGDAWHIHGK
                     IWEQGAEEPAEPQIKHEAKGLKGQGKPGIWGTPYSEQPIHFDDIQIAIELPAAS"
     gene            complement(21541..22335)
                     /locus_tag="PLP58_09285"
     CDS             complement(21541..22335)
                     /locus_tag="PLP58_09285"
                     /inference="COORDINATES: protein motif:HMM:NF013916.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ08749.1"
                     /translation="MTRQENLDIFFTDGKTVILPIDHGVAIPVPGLENPFAMIDAVSP
                     YVDGYVMNLGVAMRAADSMAGKGICLRTDVYNTRLTGDGAGSINVYGAEEAEMVGANA
                     VMNMIYPWSVSERMNFQECADIIRSSMDLDIPVILEALPHGLGQTDKYTLENVAFAAR
                     LAAELGADVVKIPYPTDAKPGDFKKVIEQVWVPVIILGGAAVNDDAALLQMTEDAMNA
                     GAAGIAMGRNVWQHKNPPAIARSLAAIVHDDADALQALSLLREPLR"
     gene            complement(22381..>22623)
                     /locus_tag="PLP58_09290"
     CDS             complement(22381..>22623)
                     /locus_tag="PLP58_09290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ08750.1"
                     /translation="LGADPGALLRLTTACDTRRERLVEVLVPGGEESGCMSALENALQ
                     PARTGVIIKSLPAPADTGAALAAAAALRRELLRSAE"
BASE COUNT         3846 a         6884 c         7479 g         4414 t
ORIGIN      
        1 tggccagccg cttggccaaa ctttccgcgt tgccgctctg actgccgaag gcgatgagca
       61 aagacgtctt tgcctgggca tgtgctgagg atgcgcccgg agagccgccg agcttaagga
      121 ctccggcgat gaatccattc agccaggcgc gttgctcggc ggtgaacggg gcgctttcag
      181 gaatgagcgg cgtgatctgc atggcggacg cggtttagaa atcaatctga agctggacgt
      241 agccgaagtg ggcgttgttg gaggctcctg tgtcggcgag gtagtggccc gcgaaaaaca
      301 cgctgtagcc cgccatcaga ccgacatggg cgttgagttt gtagctggcg gtgaggtcga
      361 gttcgctgcc gcgaaagttg ctggcctcgc gcgacacggc gttgaccgct ctcacagcag
      421 tgacgccgtt cacacgcctc caggcgtcct cgttggtgtc gttccagtag aggtggtaat
      481 caagcatcag cttgagcttg ggcgtgggca tgacgctgat gttgagctgg ggattgtgca
      541 tgttgatcca gcccgtggtg tccatgtagc cgtaaaacag gtggttcgtg ggatacaggt
      601 tttggaaggt gcccaggtcg ccgtccgtgg cgtcgctgtc gccgggactc cagttgtatt
      661 ggaggccgat gcgtggcttc cagggatggt tcagccagtt gtagccgatg ccccagtggc
      721 cggcgaaggc tctaaggttg cgtccgttgg catccccagt ctgaaaggcc atctcggttt
      781 cataatccca gccgccgagc tttttcggat cgcccttcca aagcgtgccc atcgtgacga
      841 agctggtgtc accgagagcg gggccaaagg cgccgttgcc gttgtccgtt ttgtggaaga
      901 tataaaaatc agtcgtcgtg ttgatgggca cccacttggt ggcgaggtag gctccgctga
      961 agatggtgtc gtcaggagac tgggcgtcga agatctggga cttgttccat tcatgatctt
     1021 tgaatgtgac cgggctggag gtgaacagat cgaacgccca ggattcggac gcgtagcgca
     1081 gcttcaccgc gtcaaatgtg cgcgcctggt tcaaccattc gagagggccg acgaggcgct
     1141 ggtcgccgta gctgagaaac tgtctgccaa cagtggcgct gaggccggct tttgcgtcgc
     1201 cgagctgaac gaaggctttg aggacatcaa aaacatcgtc accctccgcg ccaaaaactc
     1261 caatttcgtt cggcctgctg ccgccgatct cgcgcacatc ctggccctgg acatagattt
     1321 tgaaccaggg tgtgaacgag taacccagcc cgagccggaa acgctggaga agccaggagg
     1381 cgtcctgcgg accgttgacc gcgctgttga agtcgaagtt gttttcgcgg gcttcgaaac
     1441 gcatcttctc ctggacatcc acggtgagtt tgccgtcgaa gaaggtgatg ggggtgaagc
     1501 cattggcggg cggcggggga gctgccaatg gagcagtttg aggagagccg gcgtgggtca
     1561 gagacgccat ggccaggagg agagccagcc ttgttttcat agggtgtgtg tgttgtgttt
     1621 gggtttcctt ggctacgcgg cggctgtagg gaaaaggagt ccgtgggcgg ccatctcggc
     1681 cgccatgtcc ggcgatggat tgaattcagg ctcgcggctc ggacggatgt gggatttgtc
     1741 gttcaggaag gtgatgaggt gctcgcgcag ctcgtagtag cgcgggtctt ccatgatctc
     1801 cttgcggttg cggggacgct cgaaggggat cttcaagatg tcgccgactt cggcctcggg
     1861 gccgtcggtc atcatcacaa cgcggtcgga gaggaagatg gcctcatcga catcgtgcgt
     1921 gaccatgagc gtggtgatgc ggttgcgacg ccagaggtcg agcagcactt cttgaagctc
     1981 gtagcgtgtg agcgagtcga gcatgccgaa gggctcgtcg agcagcagca tcttcggctg
     2041 gagggcgaag gcgcgggcga tgccgacgcg ctggcgcatg ccctgggaaa gctcgccggg
     2101 gaatttgtgc atggcgtcgg ccaaacccac gatgctgagg taatactcgg ccatctggcg
     2161 gcgctcggcc ctggtggcgg tgaaatagac ctggttcacg ccgagcatga cgttttcaaa
     2221 cgcgctcatc catggcagca ggcagggcga ttggaagaca atgccgcgat ccggtcccgg
     2281 gccgacggct tccttgccgg cgaggatcat gttgcccttt gagagatcgc tcaaaccggc
     2341 gaccatcatg agcacggtgc ttttgccgca gccgctgtgg ccgatgagcg tggcaaactc
     2401 gccctccttg agcttgaggt tgaagttttt gacgatggtg gccgggcctt tgggcgtgtc
     2461 gtagatcttc caaaggcggt caagttcgag cattttggga ttgggaacac tcatgcggtc
     2521 acctccactt cttcacgttt gtccgcgttg cggcgtttcg gaccgccgcg gttcaggaat
     2581 ggcagcgagt ttttgttggt gatgtcctcg ggcaggatgt cgggcagggt gagtttcatg
     2641 gtggcctttg attttgtgcg gcccttggcg tcgaggagcg tggtgatgag ctgcgtgcgc
     2701 aattttttga atttggggtc gtgattgatg ctgcgacggt cacgcgggcg ttcgagatca
     2761 atgagcatcg gctcgccgag cgtggcgggc gcggtgggca gcagcgggat gaccttgtcg
     2821 gcgaccaaga tggcctcatc ggggtcgttg gtgatccaga tgacggtcgt tttgttgctc
     2881 tgccagatct cactgatctc gtcctggagg gtggcgcgtg tgagagcgtc gagagcgctc
     2941 aaaggctcgt ccatgagcag caccttcggg ttcatcgcca gcgtgcgggc gacggaaacg
     3001 cgctgtctca tgccgccgga gagttccttc ggaagtttgt gcgcagcgtg gctgagcttc
     3061 accatcgcga tgtgtttggc gacgtgttcc ttgcgctgct ttggcgtcca ttctttgaag
     3121 acctcatcca cggcgagcgc gatgttttcc tccacggtga gccagggcag cagcgagtag
     3181 ttctggaaga ccaaaccgcg gtccgggccg ggttccgtga tttttcgccc ttcgatgagc
     3241 gcctcacccg cgtcgggttt cacgaggccg gagatcaaat tgatcagcgt gctctttccc
     3301 gatcccgagt aaccaacgat gacgacgaat tcgccttcct cgacgctgag gttgatgttt
     3361 ttcagcactt cggaccgtga accgttgtga ccatagcctt tggaggctcc tttgatttcg
     3421 atcagcggca tatcggtttc agatggaggg ttaagcagtc ttgaagcggc cttcgacgac
     3481 gctcatcagc cggtcgagga catagccgat gacgccgatg gtgaggatgc agaggatgat
     3541 gtgggcgtag ctgttggcat tgtattgctg ccagaggaag ccgccgacgc ctggacgacc
     3601 ggtgagcatt tcgaccgcga cgatgacgag ccaggcgata ccgaggctca ggcggaagcc
     3661 ggtgaacatg taaggcagcg tggcgggcac gagcaccttc cagagagtct tccacttcga
     3721 gagtttgagc accttggcga cattcaggta gtccactggc acggcgcgga cgccgacggc
     3781 ggtgttcatc acagtgggcc acatggcgca gatggcgatg gtgaacaggg cggcggcatc
     3841 ggacgcacca aacgtcgtgc ggccatcgcc atccaagatt ttgacgccgc tgaagagaat
     3901 catgccaagc ggaagccagg cgaggggtga aacgggccgc agcacttgaa tgatgggatc
     3961 gaaggccttc gtgaacggtt ttgagagacc gagaaagaag ccgatcggcg tgccaatgac
     4021 gagcgcgatc aaatatccct gcgccacaag cacgagtgac atccaagtga agcgaaggat
     4081 gccttgatcg agctcgccgc gcttggcgaa gggctcgacg atgtaaggct tgctggctgt
     4141 ccaagtttct ccaggcgtgg gaaggtcggc ggaaagccct tcgcgcttgg tgattttgac
     4201 cttgtcgccg aaatcatcga ccttctccgt cacaacggac tttccggcga tggcatacca
     4261 gaagacgcag gtgatgacga tcgcgaccaa cggcaggaag atggagtcga gtttgtattt
     4321 ctggatgagc gggtgcatgg cggcggcgtt tggatttgtt cagaccgatc cgtcggcttg
     4381 gacggatcgg tctgatgact ttcttatcct ttgatcgtct tcacggcgaa ggacgcggcg
     4441 taggcttcga ggtcggcctt cggatcgaaa gtgacaccgt caaagagtgt ctcggcgctg
     4501 gtatcgacac cgccgtgcgc gtagccgatc tccttcatgg cctcttcata gatgtcggag
     4561 cgcatgacct gcttcgtgac gccttcgtag tccggcgcgc cttcgacgaa accccagcga
     4621 cggagctgcg tgagccacca cttgcaatac tttgcctgcg ggtagttgca gttgcgatca
     4681 ctgaagatca tgtagttcgg gtccttgaac ttgcggccgt cgcccatgag gtatttgccc
     4741 tggaggcggc cgaggatcgt ctcgggcggg cagttgatgt aggtggcggc gctgacgatg
     4801 ttcgcctgct cctcgcggtt ctccatcttg tcgagccaca cgctggcctc gtgcagcgcc
     4861 ttgatgacgg ccttgacggt ctttggattc ttcgcggcga actcctccgt gaaggcgcag
     4921 actttttcag ggtgatcctt ccacatggcc tgggtggtga tggaggtgaa gccgatgtcg
     4981 tcagcaatgg tcttcgcgtt ccagggctcg cccacgcaga agccgtccat cttgccgacc
     5041 ttcatgttgg cgaccatctg cggaggcgga acggtgataa gcgacacgtc agcgcccgcc
     5101 ccattcgcat cgccgggatt gatgccaccg gctgcaaggt agtagcgcat ccacatggca
     5161 tgcgtgcccg gcgggaaggt catcgcgaat gtcagcggcg cgcctttggc cttggcgtca
     5221 tcgacgaagg gcttcagggc tttcgggtca tcagtcacct tccccttgta ggcgttgctg
     5281 agtgaaatgg cctggccgtt gcggttgatg agccagggga tgatcatcgg tttcttcggc
     5341 gcaccgccga gtcccatcgt ggaggcgatg ggcatgccaa tcagcatgtg ggtggcctga
     5401 atgtcgccat tggagagcga gtcgcgaatc gcggcccagc tcgctccttt gctgacggtg
     5461 gagttgatgc cgtatttttt gaagagaccc ttttcatgcg ccacgacgat gctggagcaa
     5521 tcagtgaggg cgatgatgcc aaacttgaga tcggcgacct cgggagaatc atcggcgtag
     5581 gcggagccga cccagccgga aggcaggccg gagagtaggg cgccgaggcc agcctggcgg
     5641 aggatggagc ggcgggtgag cggagtggcg gtgttcatgc gtgggcgagt ggtttgcgtt
     5701 ttttgggctt cgagacgggt ttgggtgcgg cggcgggtgc ttcgggaaaa tacagggcgc
     5761 cggaggtcca gcagtcgcgc agggaggcgg cggcatcggc gcgttgcgcg ggcatgagca
     5821 ggccgtgggc gatgaactca tcgagcagcc agcgtcctcg ctcgctggta ggctcattgg
     5881 cctcgcggcg gtggaaaatg tggaagctgg acgcatccca attttgtccg gtgccggggt
     5941 cgaatgggcc gatgagggat tttttcaaca tgctctcgca gccgttgaag aagccgctgc
     6001 cggcgagcac tttgacgact tccggccggt tttcaggcgc gtcacaaaag gcgcaggact
     6061 cccgcagggc gttgatgaca gcctgggcct ggtcacgatg ccgggtgatg aacgcgcctc
     6121 ccatgatcag cgccttttca ggatgaccgg gcgcgagctg ctcgctggtg gcgacgaccc
     6181 agcccgcgcc tttctcgaca gccacggagt tccatggctc gccgacgcaa taaccgtcaa
     6241 tgagtccggc ggcgaggttt gcggccatct gcgtcggtgg caaagcgacg atgcgcacat
     6301 cctggccggg gtggatgccg ccggagatga gccaccggcg caggaggaaa tgatgcgagg
     6361 cgtagcgcga gaccatgccg aaggtgaggc ggcgcgaggt ggtgctgcga atgagttttt
     6421 tcagcgaggc ggcatcgcgc acgccgcggt tccagaggtc gcggctgagg gtgatggcat
     6481 cgccgtggag attgaagaca aagggggcga ccaccggcga gggaggcgag ctgatgccca
     6541 tgcgcatggc cagcgccaga ccggcgatgg catgggccgc gtccacctgg ccgtagagaa
     6601 gtttttcgcg gatggtggcc cagccgacct cgcaactgag ctcgacacgg acgccatgtt
     6661 tgcggaacag atcccggctg cccgccacca gaagcggggc gcagtcagcc agcgggatga
     6721 agccgatcct caccggctgg atgacaccgg tgagcgctgg cgcggggctt gatgcgtgct
     6781 tgggcatgat gacagcctca tcagcatcag aggtgccagc gtgccagggt catgcagaaa
     6841 cgacgcgcaa actttttcac atgttgtatg aatccggctc atggaacagc cgctcgacac
     6901 acgccagctc cgcgccttca tctcactcgc ccgcagcggc agcttcacgc aggcggggcg
     6961 cgagctgcat ctcacgcaga gcgcgatcag ccatgcgatc aaggcgctgg agaatgacct
     7021 gggctgccag ttgttccacc gccagggcaa gagcgtgcat ctgacgcatc acggccgcga
     7081 gctgctgccg cacgccgagt ccatccttca agagatgggc caggcgcgcg cggccctggg
     7141 cgcgctggac cgcacaccgc gcgggcggct gagcatcggc tgcacgccgg cggcctcgca
     7201 gttcatcctt cccaccgtgt tccgcgaatt taaggagagc ttccctcaat atgaaatccg
     7261 tgtcgtgccg ggggagacgc cgcagacgat cgagcgcctg ctgaacaatg aagtggatct
     7321 ggcggtgacg ctgcgcccgc cggatgcggg gcgtctggaa tgccagcctg tgctggagga
     7381 tgagctggag tttctcgtgt ctcctttgca tccgtggacg acgaagccgc cgagggtcaa
     7441 ggatgcggcg gcggagactt tcatcgtctc cagccgcagc agtctgaatt ttcaaatgat
     7501 ccaggagttc tttctgaggc agggcgtgcg cttgaaccac ttcattgagc tgggcagcag
     7561 cgaggcggcc aaggagctgg ccaaactcgg cctgggcatc gccatagccg cccgctggat
     7621 cgcgcgcgcg gagatcgagg ccgggcagct cgtgccggtg ccgctgccga agtcaaagct
     7681 gcgcaggcgc tgggtggtgg cctggctgaa aggacgtccg ttgaacctcg ccgagcgcac
     7741 cttcaccggg ttgtgcgagg aggtcggcag gaggatgctc tgactctgcc gggctcctca
     7801 cgctttcttc acgccgctca tcggatgcga tgtgacggcg tcccaggcga tctgctggag
     7861 cagccggacc aggtcgctgg actgcggacg ggcgctgagt ttctcaggca ctggcaggcc
     7921 cacgggagtg cgctggtaaa tgcaggcaaa atggcagtag ctcacgagca gggcggtgat
     7981 gtcggaggga tgcccgtgat cgtcgcggaa cagctcgctc tgtttggaca cgccgggcgc
     8041 cctgccctcg gcgacatgcc tgcgcagtga atgcagcgcc gcgctggccg gtatgatgcg
     8101 cacgctcggc cttcccaact cggtgttcaa cccggtgact tgcttctcca gcaggctgag
     8161 ccagccgttg tgggcttcga gcatgctgtc aatctcctcc gcggtgacgc tgtccctcat
     8221 ggcattggca aagccggctc cggccttgcc gtcacgcggc gcccacgatg cctgcacgag
     8281 cacgcgcagc tccggatttt tctccagacc gagcctggtg aagcggtcta ttccctcatc
     8341 cggcatcagc aggttcggtg aaagtgtgag cacatccacg atgccttcct ggagcgcctt
     8401 tttcgcctgg ttcctttcat caggcagatc ccagtgctgc tgcacgcgcg agccgccgat
     8461 catttgctgg ccggccttca aatgcggcac tcccgccgcc tgcgcgatct gcggcagcca
     8521 gtcggcggtg tagatcatgt agctgtgcgc gcagacgaag accttggtgc cctccgcgcc
     8581 gggggcgaat gaggcgggct ttgagctgct tttgccggag cccgcgcgcg ccatgaattt
     8641 gtcccgcgcc tcagggtgtt tttccacata ggccagagcc tcattgatgg aaagcgcgcc
     8701 gtctttgtcc gtgtcggcat cagggaacat cctcagcgcc tgctccagcc gcgcctcaat
     8761 gccgcttttt ttggcggcga cctgcgcgat gagttggaac gcgccgggaa cgagggcggc
     8821 gagcgggaga atcaaaaggg cgtgcttcat ggtgtgtgga tggttttgcc ttgtgtcgaa
     8881 catggatgcc aaacgcctcc cggcaactcc ggtaacaagg cccggtcttc agttgttcaa
     8941 aaacccgtgt ttggaaacca gcggctgaaa tggttgtcgt cgaggcgcgt gagcgggacg
     9001 gacagcggcg agggcacgcc gtttttcgac cagcgcagga gatcgtcttt atggccaagc
     9061 cggccgagca catcgaacac atttcccggc gagccttccg gccagccctt catcacctgc
     9121 gggtcccaga tggcgatgcc gggagcgcca tcggcgagcc aggcggcggt tttctggcac
     9181 agttccttcg gtttgcctgt cttccaagcg atgaccatcg gccaggccct cgcgctcgtg
     9241 ccctgggtga tgcggcggta gtagtccatg tcaatctggc cgggcgtctt ctcaaaggag
     9301 gtgtggtagg cgaaccaagt gacggcgatg tcatccacga gtccgagctg aatccagttt
     9361 ggcagatcaa ggccccagcg ctggttgtca gcctccctgg cgaaagtgcc gagcgagatt
     9421 ttatagcgct ctttgcggcc ctgccttgcc gctgtttcat ccagcagggc gcgcacctcc
     9481 ctcatgaagc cggtcatgac cgccgcgcgc gtgctttgga tgcgcgggtc gtcgtcttcg
     9541 actgatctgg catccgcgtt gtgcatttcc ttgaagcgca cgcaaaaagc ctcctcccac
     9601 agcatcagcg gcatgcctct gttgaaaagg aatccgactc cctcgggctg cagctccaga
     9661 gtctcgcgaa gcagctcaat caaatgccgg cgcacctccg gcacggcgta gctcatgtaa
     9721 aaagtcgggg tgccgtcgcg gtcaaggcag cgccattcgg ggtgttcgta ataaaacctg
     9781 ctgttgaaag tctcctcata cggcatcgag ccggcccagc cgccggggcg cagcatgaca
     9841 tgaaactccg cgccctgcgc cttcgcggca tcgcgcgcga ccttgagcgg gttgacgccc
     9901 gccttgaaca aggtctccag cgactttgtg tacgcggcat aggcgcccgt gggaaaatcc
     9961 accgtgccct cgccggggta ggtgcccacc ttgcttgggt agcacaccag gtcgccgccg
    10021 gtgacctgga accaccactt gccgatgtcc gtgtcctcat aaccgcggaa ctcctccgcc
    10081 aactcctcgg cagtcgttgg tttgaaaggc catatccaac tgtgcccgtc aaaggtggcg
    10141 atcgaagtgc ggtgcttcga cctctcccgg ctggctgcga cctcctccaa agtcatcggc
    10201 acgagcttca catagcaaac cgtcgccggc agtcccggca tgggggcgat ctccagcgac
    10261 tggccgttga gttccgccac gctgacaaag cactcctgga tgacagcccg ccggtttggc
    10321 agcagggcga ggttgttggc gagccgccgg aacaccggct cgtcgctgag cttcgccttg
    10381 atgccgttgc cgccgatgtt gaacccgccc gatgtcgtgg ccaggcccac atgcacggag
    10441 tgccagccct tttccagcaa tggcagcgag acgcgcggcg gattggtggc gctgtaaacc
    10501 gaaagcgccg tgcctttcat ctccgctgtc tcaaagggca gcatcttcca tttgcccttc
    10561 tgccgcctgc cggtgatcag cgcggcttga ggctgcatcc gcgaaagatc ggtgatgagg
    10621 accgccttcg atgcctgcgt gagggcgcgc gagtcatgcc tgaagtagtc ttgagcaacg
    10681 gcccgcgcgc agaacagcgc cgtcaataag cagagagtga ggcatttcat cccgttcaaa
    10741 cgtgcgggcg cgctctgaac tcgctttctt tgtcactccg gcgcggacca tccgcctcag
    10801 acttcgtcac ccagccgctt gtccgtctcc agatagcccc gcacatagtc gcgcacggcc
    10861 tcggccagag tgaaaagcgg ctcctcatat ccggaggcgc gcagtttgga aatgtccgcg
    10921 caggtgtggt actggtactt gtcccgcagg tgcgcaggca tctcgatgaa ttcgacgacc
    10981 ggctcccggc ccatggcggc gaagatggcc ccgacgagtt cgacccaggt gtgggctttg
    11041 cccgagccga ggttgaagac gccgttggcg gtggtggtct ccgccagatg cagggtcatt
    11101 ttcaccgcgt ccttcacata gaggaagtcg cgcatctggc agccgtcctg gtagtcgggg
    11161 cggtgggatt tgaaaagctg gaccctgcca gtgtcgagga tctggcggtg cgccttgtgc
    11221 acgacgctgc gcatgtccgc cttgtggtcc tcgttcgggc catagacgtt gaagtatttg
    11281 aggccggcga tgcggccgag catgccggtg cggcgggcgt gcaggtcgaa gaggtgcttg
    11341 gagtagccgt acatgttcag cgggcgcagc cggtgcggat cgtccatccg gtcgtccatg
    11401 ccctgcgcgc cgtcgccgta ggtggcggcg gaggaggcgt aaacaaagcg cgcgtcctgg
    11461 ccgagcgccc acccgcagag caccctggtc agctcgtaat tgttgcgcat gagatagtcg
    11521 gcgtccttct ccgtcgtggc ggagcaggcg ccgagatgga gcaccaggtc gaattttccg
    11581 agcgcggaag ggtcggtgcg cgcgagacgc tccaggtcgc cgccatccag gtaatcatga
    11641 aacctcagcg gcgccaggtt gcgccacttt tcatccgtgc agaggcggtc gctgacaatg
    11701 atgttctcgc agccgcgccg gttcagctcc cagacaaggg cgctgccgat gaagcccgcg
    11761 ccgccggtga cgaggatgcg agattgaggt tggatgggca tggctctgcg ttagccagaa
    11821 gcgcggcgtt ttgcaaccgg cgacttgcct tcgccggggc tggccgcctg tctctttgaa
    11881 gcccaacggg cttccgtcat gcagcccggg gttcgcagaa ccccgggtca tgcgcgaccg
    11941 acgattccca caggtcgtgg aaccctgaaa gggttccgtc acctgtacgt ccaccggccc
    12001 tcgacgatgg acggaacccc attggggttc gcccttgggc atggcgggtg tttcatcgcg
    12061 attcccgggg tagcgcttcg cgcaaccccg ggctgcatga cgcaaggccg ttggccttga
    12121 aggtgattaa gcgttgccac atacaacttg cggcggacga aatggcaatg tggccactga
    12181 cacaagcaaa ccacacacaa gcccggttgg gaaaatcacg attgcatgat gacaaagaac
    12241 tacccaaggc caaaagaagt acgggtagat gaaaaggacc gcaccaatga acggactcac
    12301 aagtccaaga attggcagaa caaaccaagc aaggttacgt cctcgacgat aaagccaaat
    12361 ccaagtcaga attgaaacgg tgaacttctg aatggtccat ccgggttcat acgggaggat
    12421 tcccatatct ccggtgccgt tatgccagcc tatgagccaa aagccaacga cacacatccc
    12481 gacggcgatc accgccacaa tcaagtgttc gatgatcgcc ttcatgattc actcacacat
    12541 gaatcggatg ccccttggcg acttcggtgg cttccttgac catctcgctg agggtcggat
    12601 gggcgtggat ggtggcttcg atttcgtccc aggtggcttc gaggttcatg gcgaggccga
    12661 tctcggcgat catctcggtg ctctcgctgc cgatcatgtg cgcgccgatg atttcgccat
    12721 gcggctcgcc gtagaggatt ttcacgaagc cttccggctc ggcggcggcg agggccttgc
    12781 cgctggcgac gtacgggaac ttgccgacct tgaacttgag gcccttttcc ttcgcggcac
    12841 gctcggtgag gccgatgctg gcgacctgcg gatggcagta ggtgcagccg gggaagacgc
    12901 cgactttctt cggcttgtgc ttggtgaaga ggccttcgac gcattgcacg gcctcgtagc
    12961 tggcgacgtg cgcgagccag ggcggcccga tgatgtcgcc cgcaccgtag atgcctttga
    13021 cgctggtttc gtagcgctcg ttggtctcca gccagccacg gtcgctgagc ttcagctcga
    13081 tgccgccggg aagcaccgga gcgacgccga tggcgacgag ggcgacctcg gcctccagcg
    13141 tttcgttggc cgcgccttcg acggtgattt tcacgccctt gccgtcctcg gaggtgccgg
    13201 tgaccttggt gttggtcagg atgcggatgc cggccttggt gaggctcttt tccagcgtct
    13261 tgctgacctc ggtgtcctcg accggaagga tgtcgggcat catttcgacg acggtgacct
    13321 tggtgccgta ggcattgaag aagtaggcga actcaatgcc gatggcaccc gcgccgatga
    13381 cgatgacgct cttcggctgc ttctccaagg tcatggcctc cttgctgccg atgacggtgg
    13441 tgccgttgaa ggagaaaccg ggcatcgggc ggctcttcgc gccggtggcg atgaggatgt
    13501 ttgcggcgtc gtgggtctcc ttcctaccat cagcagcggt cacttccacc ttgccggcgg
    13561 cggggatgct ggcggtgcct ttgaggtagt ccaccttgtt cttcttgaag aggaattcga
    13621 tgcccttgtt gagcttgtcg ctcacgccgc ggctgcggcc gatgaccttg ctccagtcat
    13681 actcgatgct gccgatcttc aggccgaact ccgccgcgcg gtgggtgagg gtgtgaaaaa
    13741 gctcggcgtt tttcagcagg gccttggtcg gaatgcaacc ccagttcagg caggtgccgc
    13801 cggcgcggtc gttttcgacg caggccacct tcttgccgag ttgcgctgcg cggatggctc
    13861 cgacgtagcc cgcaggcccg ccgccgatga cgatgaggtc gtagttcatg agtgtgtggg
    13921 gaaagacgag ggcgagagaa gcgcaggcgc gcgggcactt caaccaaaga ctaccctcgc
    13981 aggcgaatgc gcattgcccg gggcgcgggc cggttcgcaa gattcacagc agccgccaga
    14041 cgcgcccgtc ctgggcgagc agttggtcag cctccttcgg cccccagctt ccggccgggt
    14101 attcgcacat gggcggcggc gtggaggtct tgtgccaggc gttctcgatc tcgtctatga
    14161 acttccaggc gttttccacc tcgtcgcgcc gggcgaagag cgtggcatcc ccggccatcg
    14221 cgtcgagcag gaggcgctcg taggcctcgg ggctggccct gccgaagctg ctgctgtagc
    14281 ggaagtccat cttcacctgc tgcatggaga gcgcctggcc gggctgcttg cacaggatgc
    14341 gcagggcgat gccctcgtcc ggctggatgc ggatgacgag cgtgttctcg ctgttgccgg
    14401 gcagttgcgc ggtggcgaag ggcacctgtg gcggtttttt gaaatgcacg ctgatctcgg
    14461 tggccttttt cggcagttgc ttgcccacgc ggatgtaaaa cggcactccc tgccagcgcc
    14521 atgtgttcac atgcagccgc agcgccacat aggcctcggt ctttgactcg ggattcacac
    14581 ggtcctcctc gcggtagccc acgcggggca gaccgtccac gctgccggag gtgtattgcg
    14641 cgcggatcac gttcgctccc acatcggccg gtcccacgag cgggcgcagg gcgcggatga
    14701 ccttcacctt ctcatcgcgc acgctgtcgg cgctgaggtc ggtgggcggc tccatggcca
    14761 cgagggtgag cagttggaaa agatggttct gcaccatgtc gcgcagagcg cccgctgtgt
    14821 cgtagtagcc gccgcgtccg ccttccatgc ccaggttttc cgcgcaggtg atctggatgt
    14881 ggtcaatgta gcggctgttc cagttcggct cgaagagcgc gttggcaaag cgcagcacca
    14941 tgatgttctg cgccgtctcc ttgcccaggt agtggtcaat gcggtaggtg tccgcctcct
    15001 cgaaggtgcc cgcgaccacc tcgttgagcg tgcgcgcgct ttgcaggtcc ttgccgaagg
    15061 gcttctcgca cacgacgcgc gcccagcatc cgccggctcc ctggttcagg cccgcctcgc
    15121 gaatcagctc caggatgggt ttgaagtact ccggcgcgga ggccaggtaa aacaggcggt
    15181 tggcgggcgc gccgcgctcc tcgtcgaact ggtcgagcag ccgcgccagg gcgcggtagc
    15241 cctccacgtc ctcgaactcg ctgcggtggt agtgaatgct gtggctgaag ttcgcccaaa
    15301 gctcgtcgtt gtggccctgg cggctgttct tgcggtttcc cgcctccaac tccgagcgga
    15361 aaagctcgtc cgttttgtcg cgcctggcga agccgaccac tttgaattgc ggcggcagat
    15421 cgccgcaggc ggcgatgtta taaagcgccg ggatgagttt gcggtgcgtg aggtcgccgg
    15481 tggcgccaaa gatgaccacg gtgcagggtt cggcgcggtt gcggcgcagg agggtttctt
    15541 cgaaggggtt ttcgagatcg ggcatgtgcg gtgacgttgc gtacgccctg gggagagcgg
    15601 cgctgagatt agaccgcccg ccctccgctg gcaactggcg aagctgctcc aaaagccgcg
    15661 cgatgatgaa aagccttcat gccgttcatg accggaggcg tgtcttgcaa atccaggcat
    15721 tctggcgcaa gcatccgcgc ccatgcccgc caccaccatc ctgcccaccg accaaccacc
    15781 gctcctgcac gctgccgtgg accaggctgt gaagctgctg caatccggcg aagtcgtggc
    15841 tctgcccacg gagaccgttt atggcctggc cgccgacgct ttgaaccccg ccgcggtggc
    15901 gaaaatcttc gaggccaaag agcgcccggc ctttgacccg ctcatcgtcc atctgcctga
    15961 caagaagcat ctggacaccg tggtcgaggt gccgccggag gtgcaaaaac tcgtcatcca
    16021 gctcatcgag cgtttctggc ccggcccgct cacgctcgtg ctgccgaaga agccctgcgt
    16081 gcccggcctc gtcaccgccg ggctgccctc cgtggccgtg cgtgtcagcg caaatcccat
    16141 tttcaagcgc gtcgcgcagg cgctcggacg ccccctcgcc gctcccagcg ccaaccgctt
    16201 cggctccatc agccccacct cagccagcgc cgtcgcggcc gagctggacg ggcgcatccc
    16261 gctcatcctc gacggcggcg cctgcatgca cgggctggag agcaccatca tcaaagtcga
    16321 ggccgcctcg ccgaaaaacc tcatcaccat cctgcgcccc ggccccatca ctgccgagga
    16381 cttgaaagtg tatggcaagg tcatgcgcgt gacccgctcc gtggtggatg aagccactga
    16441 agcacccgga cagctcgcct cccactacgc gccgcgcact ccgctgcgcc tgctggccaa
    16501 gccctccgac ttcacccccg aacccggcaa acgctacgcc ctcctgagct accggggcga
    16561 tgaaaacgac ggcttcctcg gcctggccga ctggctgcag gtgatgattt taagccccgg
    16621 caaaggcaag ctgcctgaag ccgccgtgcg ttttttctac gcgctgcgcg agctggacaa
    16681 gctcggcgcg gacgagatca tcgccgagcc catgcatgag cacggcatgg gcgccgccat
    16741 gatggacaag ctgcgccgcg cggcggtgaa ggcggggcgg ccttccagat tgcccgaatc
    16801 ccagagtgcg gcagggacgg ttgcaccacc ctccgcatga gcgacaacgc gcccagtccg
    16861 gaccagccac gccgtcccgc agactcgaag agcgcaaccg ggacggttgc acaaccctcc
    16921 caggcccgca gcaccagcgt ggtctccatc gccatcctgt gcagccgcgt gctggggctg
    16981 gtgcgcgacc agctcctcaa cggcctcttt ggcgcgcacc tcaccggcat cttcaccgcc
    17041 gccttccgca cgccgaacat gctgcgcgac ctctttgccg aaggagcgct ctccaccgcc
    17101 ttcgtcacca ccttctcgaa aaaaatcaaa accgagggcg acgaagccgc ctggctgctc
    17161 ggcaggaaga tgctcgccct ctccatctgc tgcatgagca tggtctcgct ggcgggcatc
    17221 gccctggcgc cgtggctggt gcggctgctg aatcccggct gggatgaccc gcgcagcctc
    17281 gccatctgca cggagctggt gcgcatcatg tatcccttca tcaccctcgt ctccgtgacg
    17341 gctctcgtga tggggatgct gaactcgaaa aaggtcttct tcatcccggc gctggcctcg
    17401 gcatttttca acctcggctg catcgtcggc ggcgtcgtgc tcgcctggtt catggacccc
    17461 ggcttccgtg atggggcgat cacggagaaa ggcctgcaag cctttgccat cggcacgctg
    17521 gtgggcggcc tcatgcagct cgccatccag atcccctccc tgcgccgcgc gggcttccgc
    17581 ttcgggctgg acttcaaatg gcgggacaaa ggcgtgggcg aggtgctgca actcatgtgg
    17641 ccctccctca tcgccgcggg cggcacgcag atcggcgtgc tgctgaactc catcttcgcc
    17701 tccttcacgc ccggcaaaga gtccgccgtg gcctggctgg cgaacgccca gcgcctccag
    17761 cagctcccgc tcggcctctt cggcgtggcg gtggccaccg tgactcttcc catgctggcc
    17821 cgtctggcca ccgagggcat cacacccgcc ttccgcggcg ctttggcgaa ggggctgcgc
    17881 cttgtgcttt tcctcaccct gcccagcgcc atcggccttt cgctgctggc ggaggagatc
    17941 gtctccatcc tctttcaata cgggcgcttc accgccgctg atgtggcggc cacggcgggg
    18001 ccgctgcagg cctatgcctt cgggctggtc ttttactcag ccatcaaggt gctccagccc
    18061 gccttctaca ccatcggcag gcgcttcgtc cccatgcagg tcagcctcgg catcatcgtc
    18121 ttcaccgcca gtgtgaacgc cttcaccgtc tttgtcctcg aatgggacca caccgcgctg
    18181 gcatgggcca ccgccgcagg cctcatgctg aacttcgcca ccctctacct gtgcatgaaa
    18241 aaattcgcca acggcctgga gacccgcgcg ctgctcggcg cgctcgtcag gctggccgca
    18301 ggtgtcgcgt gcatggcgct tgtctgcctc gccgcaaaag ccaccctgct ctccggctgg
    18361 gcgggcatga tcctgctcaa caaagcggcc gctctcggcg gcacggtcac cgtcgcggcg
    18421 gcggtgtact tttatgtcac gcaaaagctc cgcgtcgagg aggcgggcga gttcaccggc
    18481 atcctcaccc gccgttttcg ccgcaagtaa gccatgaaaa accaacgcat ctggatcacc
    18541 accgccaccc tgctggccct catctggggc gcgctggccg tggtcatgcg ccagaccgac
    18601 ccgctcgtct catggccgca aaaggtgctc gacctcgccg gggccgcgcc ctggcatgac
    18661 ggcaaaaccg ccagcgaatc ccagcgccgg ctgcatctcc acgaggtcat caccaggctc
    18721 aaccagctcg acttccagca gcgccgcgcg ctgcgagagg acgggcagga catcctggac
    18781 aaattcttcg cctcgctcac ggaggaggag cagaaggagt atgtggaccg caccgtcgag
    18841 ccttattttg aaaccatcag caaaggcatc aagctcctgc ccgaggatga gcgcaaacgc
    18901 atggtggcgc gcatgcgcgg cgacctgcgc aacctgcgcg ccaattccgc cgatggcgac
    18961 cgcctcagcg accaggaccg cgagtttttc gagttcatgg tggccgagga ccccgtgctt
    19021 tttctgcgcg aggcgccggt gaaaatgaaa atggagctgg cgcccgtcat cgaggacatg
    19081 cagtcccggc tgcagggttt caggcggtga ctcaggatga cacatccgcg cgccgcgcgc
    19141 gttgagatgc ggatgccgat ccgcctcatt ctcagtcttg ctcttctgtc cgcgcccgtg
    19201 ctcgccgcca agcccccgaa catcgtctgg atcgtcgccg atgacatgtc gccggacacc
    19261 gcggcctacg gcatgacagg cgtgagcacg ccgcatctgg accgcctggc ggcggagggg
    19321 gcgcgcttca cgcgcgctta tgccagcgcg ccggtgtgca gctcctcgcg ctccgctttc
    19381 attctcggcg tcatgcagac caccaccggc ctgcacgcgc atgatgtgga gaatccccag
    19441 cccctgcccg cgccctggcg gcacctgccc ggcctgctgc gcgatgccgg ctggcatgtg
    19501 accaacgcgc ccgcgcccgg caccgagcgc ggcgggcgcg ccgtgaagaa agccaagacg
    19561 cattacaact tcgcccatga ccccgccggg atgtatgacg gcgacgactg gcgcaagcgc
    19621 cgccccggcc agccgttctt cgcgcaattc cagatcgtcg agccgcaccg cccctttccc
    19681 atccccgaag agtatgacga cgccctcatg gcgcggatga gcatcccgcc gaactacccg
    19741 gaccacccgc tcgtccgccg cgactggtat gcctacctgc gcagcgtcga agtggtggac
    19801 aagcgcgtgg gggacatcct ccagcaactt gccggcgaag gcgtgctcga tgacaccctc
    19861 gtcatcttct tcgccgatca tgggcggccc atgccctggg gaaagcagtg gctcaccatt
    19921 gagggattgc gcgtgcccct gctcatgcgc ggccccggtg tggaggccgg cagggtggag
    19981 gagcgcctgg ccagcctcat tgacctggcc cccaccgcgc tccagttggc ggggctgccg
    20041 ctgccggagt ggatgcaggg ccgtccgctg ctgggcgcgc ctttcccgga gcgcgagcgc
    20101 atcttcgccg cgcgcgaccg ttgcggtgat gcgcaggacc acatccgcgc ggtcatcacg
    20161 gcggaccatc ttttcgcgcg tcactttcat cccggtttat ccttcatgaa ctggtccggc
    20221 tacaaagagg ccagctaccc ggccatgccg ctcctgcgcg agctggccgc cgccggctcc
    20281 ctgacggaaa tgcaggcgcg ctatctaaag ccggaccgcc cgccgttgga actgcatgat
    20341 ttgaaaagcg atccgctcgg tgtcaccggt gccgcattgt ccggtgacaa tgcccgcgtg
    20401 cttgcgcggc tctcaggcga actcgaccag tggatcaagg acagcggcga ccgcggcggc
    20461 ctgcccgacc cggccaccga gccgggcatg gaggaaatcc ggaaatccaa gcgccaggat
    20521 tatcagcgga cctgggagcg gcgcggtctc ggccccgaac ccagcgacgc agagcgtgtg
    20581 tcatggtggc tgcggcaata cggcctggct gagccatctc cgcccccgcg ctgagagcgg
    20641 cggctttgat gccgcggcaa ccaggatatg ccccgcgtca tcatgatttg tgatccctcg
    20701 tttggagcga tggattgttg gagtggcaat actcaagcac tccaaaatcc caatggcttg
    20761 aattcacaaa aagtggtgac gctttgcatg gttgcgccac ccaggcggac gggtcaggag
    20821 gcggcgggca actcaatggc gatctggatg tcgtcgaaat ggatgggctg ctcggagtag
    20881 ggggtgcccc agatgccggg cttgccctgt cctttgaggc ctttggcttc gtgcttgatc
    20941 tgcggctcgg cgggttcctc cgcgccctgc tcccagattt tgccgtggat gtgccaggcg
    21001 tcgccctcgc cgcgtctggc ttcgagcttg agccagaccc aggcctcgct ggtccaggag
    21061 tgggggacgg tggctaggat ttcgtcgttt ttgtgcagct cgagctggcg tttggcgggg
    21121 ttgaggatga ggcggtgccc gctcatgccg tggacggaga tgccgaagcg cgggttgctt
    21181 cgggcgcggc gggcgctgaa gacccgggcc tggatgctgc tctcgccggc ggcggagctg
    21241 ccgagctggg cggaggcgtc ggtgacggct tcgggcagga cggtgatggc gttgttgccg
    21301 tccttggcgg tgatttggat tttgccgtcc acgacgaaga cctccttggg cgggtcgccg
    21361 ggctgccagt cgtctgcatt gagggtggcg cgctggattt cgccggcatg cagatgggag
    21421 aaggcgagcg cgaggagaaa gagggcgggc tttttcatga cgtggatgaa gaacgggcgc
    21481 ggcgcggttt ttttcatgag agtgcggcgc cttccgaagc aggcgcggcg gggcgggcag
    21541 tcagcgcagc ggctcgcgca ggagggagag ggcctggagg gcgtccgcgt cgtcatggac
    21601 gatggcggcg aggctgcgtg cgatggcggg gggatttttg tgctgccaga cattccgccc
    21661 catggcgatg cccgcggcgc ccgcgttcat ggcgtcctcg gtcatttgca ggagggcggc
    21721 gtcgtcgttg actgcggctc cgccgaggat gatgacgggg acccagactt gctcgatgac
    21781 ttttttgaag tcgccgggtt tggcgtcggt gggatagggg atcttgacga catcggcgcc
    21841 aagctcggcg gcgaggcggg cggcgaaggc gacgttttcg agggtgtatt tgtcggtctg
    21901 gccgagaccg tggggcagcg cctccaggat gacggggatg tccagatcca tgctgctgcg
    21961 gatgatgtcg gcgcattcct ggaagttcat gcgctcgctg acggaccacg ggtagatcat
    22021 gttcatgacg gcgttggcgc cgaccatttc ggcctcctcg gcgccataga cgttgatgct
    22081 gcccgcgccg tcgccggtga ggcgggtgtt atagacatcg gtgcgcaggc agatgccctt
    22141 gccggccatg gagtcggcgg cgcgcatggc cacgccgagg ttcatgacgt aaccgtccac
    22201 atagggactg acggcgtcta tcatggcgaa gggattctcc agtccgggca cggggatggc
    22261 gactccgtgg tcaatgggca ggatgacggt tttgccgtcg gtgaagaaga tgtcgaggtt
    22321 ttcctgtcgg gtcatgaggg tggctggatc aatggggcgg aatgcggggg tgtaaagcgg
    22381 ctactcggcg gagcgcaaaa gctccctgcg cagcgcggcg gcggccgcga gggccgcgcc
    22441 cgtgtcagcg ggggcgggca gggacttgat gatcacgccg gtgcgggcgg gctggagggc
    22501 gttttcaagg gcgctcatgc agccgctctc ctccccgccg ggaacgagca cctcgacaag
    22561 gcgctcgcgc cgcgtgtcgc aggcggtggt gaggcggagc agcgcgccgg ggtccgcgcc
    22621 gag
//
LOCUS       DAOMYJ010000069        22487 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Prosthecobacter sp. isolate AMR_MDS_5629
            NODE_829_length_22487_cov_22.567404, whole genome shotgun sequence.
ACCESSION   DAOMYJ010000069 DAOMYJ010000000
VERSION     DAOMYJ010000069.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867850
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prosthecobacter sp. (wastewater metagenome)
  ORGANISM  Prosthecobacter sp.
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Verrucomicrobiaceae; Prosthecobacter.
REFERENCE   1  (bases 1 to 22487)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 22487)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.30
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:34:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,742
            CDSs (total)                      :: 4,684
            Genes (coding)                    :: 4,676
            CDSs (with protein)               :: 4,676
            Genes (RNA)                       :: 58
            tRNAs                             :: 55
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22487
                     /organism="Prosthecobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_829_length_22487_cov_22.567404"
                     /isolate="AMR_MDS_5629"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1965333"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1101
                     /locus_tag="PLP58_09295"
     CDS             <1..1101
                     /locus_tag="PLP58_09295"
                     /inference="COORDINATES: protein motif:HMM:NF015722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HRJ08751.1"
                     /translation="TSGSTSTSATYRIAGLPEGETLNIRSGPGTQHPVVAELRAGDSP
                     VKITGPLVMNGDAEWAPIAFLGGKGWARTKYLAPGAAASAASASINSVKAEAGPGQGA
                     GMDARNASRENPFVNSLGMKFVPVVKYQSGKRVLFSIWETRRQDYAAYAEAVSGEDDK
                     WRDTRYFGLPAGHADDHPVIGVGWEDAQSFLAWLTRRERASGRISPGAEYRLPTDLEW
                     SHAIGIADREAANATPQEKDGKLSGVYIWGTGSMPPPDRAGNFADSTLLARRSSAKVM
                     AGYQDGFATTAPVGSFPPNRFGLHDLGGNVWEWCQDLYDPKDPKYRILRGNSYDSGND
                     AEQLNISYRGILAPDFRGDAAGFRCVLVLPAP"
     gene            1165..1770
                     /locus_tag="PLP58_09300"
     CDS             1165..1770
                     /locus_tag="PLP58_09300"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018968598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0016018 - cyclosporin A binding [Evidence
                     IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HRJ08752.1"
                     /translation="MSQKTPLLRLLPRALAILALGLPVAGHSAEPAAAQDIRIVMKTS
                     KGSISATLFASKVPMTAASFLNLAKRKYYNGITFHRVIPDFMIQGGDPTGTGRGGPGY
                     RFADEIDPGLLHTGPGIFSMANAGPGTNGSQFFITHKETPWLNGKHAVFGKVTEGQSV
                     VDAIAIGDKILEIEILDSTDALFAAQKSKLDEWNAVLDKQP"
     gene            complement(1919..3001)
                     /locus_tag="PLP58_09305"
     CDS             complement(1919..3001)
                     /locus_tag="PLP58_09305"
                     /inference="COORDINATES: protein motif:HMM:NF014893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HRJ08753.1"
                     /translation="MAIKVGIIGAGGMLQYHAAGFRQAGAEIVAVADAAPGAAAKAAE
                     KWGIPNAYDSVDAMLKGSPEIKAVSVIVPNKFHKPLTVQCLKAGKHVFSEKPPAMNAK
                     EVKEIIAAAKAAKKKVLFNFNNRARPESQAMMKYITDGTVGKINSAQAKWIRRTGIPG
                     FGGWFTTKELSGGGPLIDLLHMIDLAMYFMGYPEPKHVLGQTFSDFITDKQFKGPWGI
                     PDRADGITDVENAAHGFVTFKTGQVLSLQVSWAEMVKREEVSVVFQGTKAGGKVERLF
                     GIDGLDNTALDTCELYVQENGNSVNRGIVTPACEDMGRIGSAANFIEAIQGKAAPLNT
                     PEQALRLMQVIDAIYASAKTGKPVSI"
     gene            complement(3115..4395)
                     /locus_tag="PLP58_09310"
     CDS             complement(3115..4395)
                     /locus_tag="PLP58_09310"
                     /inference="COORDINATES: protein motif:HMM:NF012863.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDSL-type esterase/lipase family protein"
                     /protein_id="HRJ08754.1"
                     /translation="MLLLSAALCQRALAAECLVVGDSLTKEYEVEFPALYPANPASWS
                     ARNWAEILHERRNNWFDLGRFSAYADPRLTGHEHNWAFPGATTQEIRGQLGDILNFWW
                     IMELEGQLEDEAERVVIFAGGNDVDSYYGSIYNGASAAPHINATRDNLQWIVNYVRGV
                     KSSMHIVLVSVPHLGCSPKVQTEHPSDPVKTARVTAALDSLNAQLASFAASKGIGFAP
                     GVYELTRAMITDPFRIGGIEFYKQADADSRARYVFSGDGFHPATSAQGKIAQIILEAF
                     RSKYLTTQIPPLTDREIVSQILGLDPDIPFHEWMATQEVPAGAGGLLDDPDGDGLANA
                     VEFALEEGAAGLFSPGLFAPRVEHSGGQARLRWTWKERGIASEWAAARLQHSADLRAW
                     SEVDSALITLNMDGSQTVRLPLAGRGFLRLLVAQ"
     gene            complement(4468..8997)
                     /locus_tag="PLP58_09315"
     CDS             complement(4468..8997)
                     /locus_tag="PLP58_09315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009960786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="discoidin domain-containing protein"
                     /protein_id="HRJ08755.1"
                     /translation="MLRLTLPFLILLQALSLKAAEPLKALLITGGCCHDYAKQHEVIA
                     KGIQSRANVRVDVIWTDDKSTNPPLPVYDKADWAKSYDIIIHDECAASMNNKETLTRI
                     LDAHQTIPSIQLHCAMHSFRTGEDRWFKRLGLQSNSHGPQEPIAITFVDKEHPITKTL
                     ADWTTIKEELYNNVNVFDAHPLAMGRQLVKGKAVDAIVAWTNEKAGARSFSTTIGHNT
                     ETVADARYLDLITRGLLWACDKLTPDYLVPFTGQNKVTFVAAKPAEAPKPAPAPKNAT
                     GIKASASSEETGKNNFAWRAVDNDENTRWCASGPDYPQWLQLELEKPQTLTGIETSWE
                     NNGVYQFKVEGSADGKTWTTLFDGSANEKNAPYSNDFAKADGIRFVKVHALGKKSGGW
                     ASIREVRLKGPGIKAVTAKPSNKSDLSDPSDKTDYSKSGNVPPRIVKLTPEQEAAILK
                     DVKVADGFEATLFANSAAANYPVFVAAEPDGTLYVSSDGNGSLGRNPKRGRVIRLRDL
                     DGDGRADETKVFCEVDAPRGLVWDHDRLYLVHPPHLSEFIDADHDGVAEKQNILVKDI
                     AFGYKDRPADHTTNGLSLGIDGWLYIAGGDFGFMKATGTDGKTLQHRGGGVIRVRPDG
                     TGLEIYSTGTRNILEVAISPLMDIFARDNTNDGGGWNVRFHHFTGLDDHGYPRLYKNF
                     NDECIQPLADYGGGSGCGAVYIDEPGFGTWNNAPFTCDWGSGALWHHQVKPKGATFEE
                     TRKPEPFIKMTRPTDADVDGLSRVYCASWKGATFDWAGPDVGYIVQVRPKGFKAEPLP
                     DFAKAKDEELVKVLESASNRRRMEAQRELVRRDSPSRLREEKFSLTTRETQIKAIDSR
                     KEAASATNQTLAAFAADKSNDLSARVAALFGMTLRLAIVPWPEDLREISKLNADSALS
                     SFVLRAGADAMFLGDRSEMMDVRNSFAIGLKSKDSRTRFEAVVSITRQLSNYLQWPGL
                     SMVGMSKAEQHKAMIRDLSAPMTDHLDDDDPTIAHVAFRALAMMNAHEANFATVDNPN
                     IQSARRKGALLALMHIHTLEVVTGLIERLGKATDPAMRQGILAALCRLHFKEGEWKGD
                     SWGTRPDTRGPYYQPEAWGETPKIAVALKDALAKATPEEAAFLVKELNRNRIQFNEAQ
                     QRILELAKKDPKVLPELAAQMAVAEEVPVEAVPLLVGLVRQVGPVGPATLSQAIIALS
                     KTDSADGVSATLAALELLKKLPESGKDYDAALAAFLNAPKLENHHQLVEQIAERGEWP
                     VSVYANAALLNLANRKTGSPESIQLTQKALDKGWADPKHRKLIIDAISASKHMPSAAK
                     VLAALDDPDAEVKGAAERAAKAMRITKIEDKTPKIMTLKPEEVLAAVLKEKGNVALGE
                     QIFTKATCVACHTVKETEAQKGPYLGNIAQTYKRPELAEAILDPNKTIAQGFASEMIT
                     MKDGTAQMGFITQEGANEVKLRNIAAQEFTFKTADIKERQKLPMSMMPPGLMMNFTVL
                     EFASLLDYLESLAKH"
     gene            complement(9132..9821)
                     /locus_tag="PLP58_09320"
     CDS             complement(9132..9821)
                     /locus_tag="PLP58_09320"
                     /inference="COORDINATES: protein motif:HMM:NF018038.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ThuA domain-containing protein"
                     /protein_id="HRJ08756.1"
                     /translation="MAVEEPDNYDAVNSMNAFAANELRPQGHQVTVVVGDKPVKHHFE
                     GLVAALKDADLLILFSRRRFLPKEQMETVRAHLNAGKPLLGIRTANHAFIPRPKDTVD
                     AGLTIWPEFTHDVLGGENTGYETKGLPYTVSVIDGIKTPLLEGVNTANIRGYQSLYKV
                     LPLAADATPILIGTAGAGASTPPQPVAWTRSYGANKARVFYTSLGAPEDMRIADVRRL
                     LANAVKWTLEK"
     gene            complement(9952..11241)
                     /locus_tag="PLP58_09325"
     CDS             complement(9952..11241)
                     /locus_tag="PLP58_09325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009961958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1501 domain-containing protein"
                     /protein_id="HRJ08757.1"
                     /translation="MKRRSLLKLAGAAGVAANAELALIAKEAAHVAHFPMGKAEHCIH
                     IWLGGGAAQVDTWDPKALGDAKAKKAGSYYPSIETSVPGVKVTEHLSRCAKLMEHITA
                     MRTVNHDVIDEHAAASNRMHTGRNVSGTTVYPSLGSMIASERGAAAEGVPAYVLIGYP
                     NFSRGPGFLGAKAGFLYLLDTEAGPAGLKRPEHILDPRQSRREQLLGTMRQSAKELRS
                     SPILDEYDTALQESLALAGPEFMQVFDLKSEKGELRQAYGSEFGQRCLLARRLIQRGV
                     RFIEVSHNLNFVNGTGWDTHNQGQLKQHVLIQDLDQALSTLITDLKANGLLDKTLIVV
                     NTEFGRPSDFDGGGGRGHQGSAFSVVLAGGGLKHRGAWGVTDELSKMPVENPCGVPDL
                     FATILADLGINPSKELYDGDRPVPITDRGQPVKALFG"
     gene            complement(11264..14884)
                     /locus_tag="PLP58_09330"
     CDS             complement(11264..14884)
                     /locus_tag="PLP58_09330"
                     /inference="COORDINATES: protein motif:HMM:NF019223.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1553 domain-containing protein"
                     /protein_id="HRJ08758.1"
                     /translation="MSLLKAFPGLCILASTAFAADPLPVAHWSFDGGKLEIGKAEGGV
                     VFGQEGPSPKQFKDIPANNKAALFSGAKGSFIRVADPGEKSLFDFDNGDSITIEAWVN
                     PASAPKGGNVYILGKGRTSNPGQEANNQNWGLRLWDSGGFLRPSFLFRSRKDGDHAGD
                     WHRWTTTGGIAAGSGWHHVAVTYEFGKPETIRGYIDGELAPGAWDMGGATKQAPVVDN
                     DEVWLGTSMGRKESVSFDGLMDEVRLYRGILPEKTIAARYPVLPYTPVLPEGGLPKDK
                     VRVEIVENIGKAAKWPRQYPAPVDVYEEDVFGFFQIAQKYTDTGVRAARSNPYLLRAM
                     AEVTLPAGKQELLMRVRGHGRLWMDGKVIGEILFGNTGGGAHNEVEHAEAERTGPALR
                     LLGPGDRETRITIKSDGKPHVYVFEMIAGNGRVRTTLGETSLSLRTADGQQVLFTPSE
                     RAVPLTDEGWDAYRKERLAFYTQLDQQRRIALRAKEDGFWATRHAEAKKVIAAKPALK
                     NAEGIDALLAASWAKANAASAKAAGGIDFEKQIKPILAENCYRCHEEKAKGGLRLNTL
                     EAAQKGGDSGDPAIVPGKPAESRLISAIHPDAGDDLMPPKGDPLSEAQRKVLADWIAQ
                     GASYASAGKKIEPAPLTDDLEFLRRVTLDTVGVVPSAEEVAAFLAEDSPQRRERAIDR
                     LLNDPRRADHWTAYWQDVLAENPNILKPSLNNSGPFRYWIHEALSDNLPMDRFVTELI
                     MMEGGVLAGGPAGFSLAAQNDVPMAAKAHILSTAFLGQEMTCARCHDSPYHQSKQRDL
                     FEMAAMLNRKEITLPETSSVPMSTFAGRKPLIEITLKPGEKVSPGWPALFEKTLMADE
                     KAAAPHKSSDSRAQLAALITSPHNGRFAQVIVNRVWKQFLGRGFVEPVDDWEASKPTH
                     PELLEWLARDFVAHGYDLKHLERRILTSEAYQRRPRPLEKGATPDFAAPLPRRMTAEQ
                     IVDSLFASVGKNLDSEELTMDNDGTQSESAMISLGHPRRAWEFTSLSNERDRPSLAIP
                     KAQIIVDVLENFGWRPSRQEPKSVRETAANVRQPAILANGALGRWVTTLDEKSAITAL
                     ALDEKLSDAQLVDQIFLRLLTRHPTEAERNSFAALISPGFKERIIPEGKRPSPVKLPP
                     LKYVAWSNHLSAGANSIKIELEKRAREGNPPTTALRPEWRERLEDLLWAVLNSPEFVH
                     LP"
     gene            complement(15006..15785)
                     /locus_tag="PLP58_09335"
     CDS             complement(15006..15785)
                     /locus_tag="PLP58_09335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ08759.1"
                     /translation="MPLLPTTLAYPASEIPKLEALRQLFQDWLHASVEAAPEMKEQIE
                     GMVFDGFYPHYFSRKTRILFIGREAREIEGFNYLDLLHAAYRTGKRIGSQHLNCNLFH
                     RRMLYMAYGLLNGLLEWKEIPDADQIGDSFATAEGVSFAFMNLSKFSNESEFWPSNWR
                     MIGDAVRLATQTRNFIAEEVAILQPEIVITMNLGDYFSSFGQEVTPLASASRDVSSWT
                     LMSNGHKALLLDCFHFSAPGKRDLDHYYLPIAEVVKLRFGG"
     gene            complement(16617..18092)
                     /locus_tag="PLP58_09340"
     CDS             complement(16617..18092)
                     /locus_tag="PLP58_09340"
                     /inference="COORDINATES: protein motif:HMM:NF021622.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ08760.1"
                     /translation="MDTPLTEILSDGWLVDNTVHTAQDGVFRAGRNGEPVNIRQRGKG
                     WTHPLSGWSVLRSAAALNTQESRLVFRVPEAEYEHNTEDAFVTLSGFDPLNFTLTTGN
                     LIGCVKSDGCALSVSSRFGDEFLKYIIADADGFAELPEHGGTERGGYEWLLNYLWLVK
                     LKKAFRLGLPKAYETSTESLTQVRGRLDPVDYFLNQKLARYRCTYREHSYDNDATRLI
                     ARTLEHLDSHDFLRGAHTLNQTFQIATEGRHHALKKLLATQPVRNPYYADYNPVIALS
                     KQILRNELSDFGDKSKTSAFFFDVSMLFEFFIRKLLKRAGVVFQSKNDRQLTIPSGLL
                     NGYTRRSLIPDLLFDINGATYVFDVKYKSFKFSGRNGGGVSREDLFQLHTYIGQVSNR
                     HRVAGCGFIYPIRESRWHDSGLEATSGILSDAIIQGGRTIPFHVVFLKVPEQQAIPNE
                     EWPHLFQSSFKDISTNFVCKFIERLISTRNDSVNYYTEQAI"
     gene            complement(18092..19831)
                     /locus_tag="PLP58_09345"
     CDS             complement(18092..19831)
                     /locus_tag="PLP58_09345"
                     /inference="COORDINATES: protein motif:HMM:NF019348.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HRJ08761.1"
                     /translation="MSRLRNLYTYLITNNAAEVDRGWLADYIEFTSEVEKVRASLKLQ
                     LDIRDKSTYAGTRFELTSDPWAAFAEHLLYKKANGISSRGQSVLSDDNFQLFIAEPNF
                     VAALAELIRDPSRENFSKYWEVWEATRQKHRAKANPLLINRTVAACTTKVTSTVNVPD
                     FDRVYRWLVGEGILERLSDPNAGWYDRNVQVMEMLRAAFAEELEIGETSEQLLSMFVW
                     YLFENIENISNPFTLKKQVIKYGAPGTGKTYTAMQSTKLFFDIWRDKYPNIAPELTHL
                     QCIKVVQFHPSYGYEDFIEGLRPELRNGQPQLALQNGVFKEFCRRAGTWEIEVHGIPS
                     AGPQLAADWQGLTVGDLKPHIGKELKHPSWDGIGTLGDSEKVADAVPPFFFIIDEINR
                     AELSRVLGELMFAIEYRGVKGRISTQYAQLNTKETAMLTTGAEFWFFIPHNVYIIGTM
                     NTIDRSVESFDLALRRRFRWERADPDIDAVRLHLQKRDAEPGNGSHPWAGLADDLAQL
                     NERIRKEEILGADYEIGHAYLMNLRYPPTLTHTEVRRSVWGDSIRPLLEEYLRGSGRA
                     ETLIPEFQKAFGF"
     gene            complement(19940..20593)
                     /locus_tag="PLP58_09350"
     CDS             complement(19940..20593)
                     /locus_tag="PLP58_09350"
                     /inference="COORDINATES: protein motif:HMM:NF013122.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease domain-containing protein"
                     /protein_id="HRJ08762.1"
                     /translation="MPRLLMKIVALDFETANHSPLSACALGLAIFESGCGFCSTSEHG
                     AQASCLCEQAGRLFPIKETPPKPQPLSFQLIESPYWLIKPPKGHGWFKEEWTEDIHGI
                     SWFDVRDQPEFGGIAPEAIPHLASADLVVAHNASFDLRVLHALLEHFEIPAPPFRHEC
                     TLHLARQTWPHLPNHQLSTLAAHLGIELNHHHAQSDAIAAGRVMLEMINPFERKHGE"
     gene            complement(20672..21748)
                     /locus_tag="PLP58_09355"
     CDS             complement(20672..21748)
                     /locus_tag="PLP58_09355"
                     /inference="COORDINATES: protein motif:HMM:NF012234.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HRJ08763.1"
                     /translation="MPETRPAQLVCEMAEAGAFSPHLGRMHGPVLAAFGGGPMHECRM
                     DGSYDLQKLISIHSRHLFRSIQRFHSREETLFWDDLTFRMGSHAFVYADESRIIACAD
                     TAQTAEQLARQFHEDGLQPPVPRGGRYRLIRTGRDISTQNVPLSPDTVLDDARLSLHY
                     GPGFVEWHQQYLGLLRRHRHGLSLLEGPPGTGKTSYLRHLMGALTETHRFYFVPNGSL
                     SVLTHPEFVGFWADERRRHDKENFVVILEDSDTAILARGPDNRDQVSAILNLSDGMLA
                     DFLRLQIICTINCRASDIDPALLRPGRLLTHRLFDRLPHAAAASLAAHLGKTLPGHGD
                     YSLAEIYADEPPAPQPRRAIGFTA"
     gene            complement(21868..>22487)
                     /locus_tag="PLP58_09360"
     CDS             complement(21868..>22487)
                     /locus_tag="PLP58_09360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ08764.1"
                     /translation="GNYEDVVNTQAKFAQAELALKADPAFQKDWLRIKAAFDVSAYQD
                     YKGVLRRTMGAERNMRPEFRARLIRPGEAFRLAFDAFCLRWNLYGMMHDDPLLLKLAV
                     NVTPHGTMILIPSFWSFDPKRDMRWDAINKLHRLRVKGRQGAALAEGLAERMAQAAKL
                     RMLDAEAKSKKLRGAARHRFLCQGLGWVEDTDAKRLQRLRKMLQD"
BASE COUNT         4135 a         6721 c         7010 g         4621 t
ORIGIN      
        1 accagcggca gcaccagcac ctccgccacc taccgcatcg ccggcctgcc cgaaggcgag
       61 acgctcaaca tccgcagcgg ccccggcacg cagcaccccg tcgtcgccga gctgcgcgcc
      121 ggcgacagcc ccgtgaaaat caccggcccc ctcgtcatga acggcgatgc cgaatgggcg
      181 cccatcgcct tcctcggcgg caagggctgg gcgcgcacca aatacctggc acccggcgcg
      241 gcggccagcg ccgccagcgc ctccatcaac agtgtcaagg cggaggccgg gccgggccag
      301 ggagccggca tggacgcccg gaacgccagc cgggaaaacc ccttcgtcaa cagcctcggc
      361 atgaaattcg tccccgtcgt caaataccag agcggaaaac gggtgctctt ttccatctgg
      421 gagacccgcc gccaggacta cgccgcctac gcggaggcag tgagcggcga ggacgacaag
      481 tggcgggaca cacgctattt tggcctccct gccggccatg cggacgacca cccagtcatc
      541 ggcgtcggtt gggaggacgc ccagtccttc ctcgcctggc tgacgcgcag ggagcgcgct
      601 tccggccgca tcagccccgg cgctgaatac cgcctgccca ccgacctcga atggagccac
      661 gcgatcggca tcgccgaccg cgaagccgcc aacgccaccc cgcaggaaaa agacggcaag
      721 ctctccggcg tctacatctg gggcaccggc agcatgcccc cgccagaccg ggccggcaat
      781 tttgccgaca gcaccttgct ggctcgtcgc agctcggcga aagtcatggc aggctatcag
      841 gacggctttg ccaccaccgc ccccgtcggc agcttcccgc ccaaccgctt cggtctccat
      901 gacctcggcg gcaacgtctg ggaatggtgc caggacttgt acgatcccaa ggaccccaaa
      961 taccgaattc tgcgcggcaa ctcctacgac tcaggcaacg atgctgagca gctcaacatc
     1021 agctaccgcg gcatcctggc tccggatttt cgtggcgatg ccgccggctt ccgctgcgtg
     1081 ctcgtcctcc cggccccgtg acgcgccgcg catgagccgc agattggcgt tgacctttgg
     1141 cgcggcattt tccattttcg cggcatgagc caaaaaactc ccctccttcg tctcctcccc
     1201 cgcgcgcttg ccatcctcgc cctcggcctc cctgtggccg gccacagcgc cgagcccgcc
     1261 gccgcgcagg acatacgcat cgtcatgaaa accagcaagg gcagcatctc ggccaccctc
     1321 ttcgcctcga aggtgcccat gaccgccgcc agcttcctga acctggccaa acgcaaatac
     1381 tacaacggca tcaccttcca ccgcgtcatc cccgacttca tgatccaggg cggcgacccc
     1441 accggcaccg gcaggggcgg ccccggctac aggttcgccg acgagattga ccccggcctg
     1501 ctgcacaccg gcccgggcat cttctccatg gccaacgccg gccccggcac caacggcagc
     1561 cagttcttca tcacccacaa agagaccccc tggctcaacg gcaaacacgc cgtctttggc
     1621 aaagtcaccg aaggccagtc cgtggtggac gccatcgcca tcggcgacaa aatcctcgag
     1681 atcgaaatcc tcgactccac cgacgccctg tttgccgccc agaagagcaa gctcgatgag
     1741 tggaacgccg tcctcgacaa gcagccctga taccttcagc caaggcgctt ggcaccggcg
     1801 ccaggcaggg tggccggccg cctgcgcgcc ttggccgcca tgcctcttgt caggggctgg
     1861 aacgagagca tcgcaggttt cgcaaacgcc tcccggaacc gggcattttt tgccaggatc
     1921 aaatggacac cggcttccct gtcttcgcgg aggcgtaaat ggcgtcaatg acctgcatga
     1981 ggcggagggc ctgctcgggg gtgttgagcg gggcggcctt gccctggatg gcttcaatga
     2041 agttcgcggc ggagccgatg cggcccatgt cctcgcaggc gggggtgacg atgccgcggt
     2101 tgacgctgtt gccgttttcc tggacgtaga gttcgcaggt gtcgagggcg gtgttgtcga
     2161 ggccgtcaat gccgaagagt cgctcgactt tgccgccggc cttggtgccc tggaaaacga
     2221 cggagacctc ctcgcgcttc accatctcgg cccaggagac ctggaggctg agcacctggc
     2281 cggtcttgaa agtgacgaag ccgtgcgcgg cattttcaac gtcggtgatg ccgtcggcgc
     2341 ggtcagggat gccccatggg cctttgaact gcttgtcggt gatgaagtcg gagaaagtct
     2401 ggccgagcac atgcttcggc tcagggtagc ccatgaagta catggcaagg tcaatcatgt
     2461 gcaggaggtc aatgagcggg ccgccgccgg agagttcctt cgtggtgaac cagccgccga
     2521 agccggggat gccggtgcgg cggatccatt tggcttgggc ggagttgatt ttgccgacgg
     2581 tgccatcggt gatgtacttc atcatggcct gcgattcggg gcgggcgcgg ttgttgaagt
     2641 tgaagaggac tttctttttc gcggccttgg cggcggcgat gatctccttc acttccttgg
     2701 cgttcatcgc cgggggtttc tcggaaaaga cgtgcttgcc ggccttgagg cactggacgg
     2761 tgaggggctt gtgaaatttg ttcggcacga tgacgctcac cgccttgatc tccgggctgc
     2821 ccttgagcat ggcgtccacg gaatcatagg cattcgggat gccccatttc tcggcggcct
     2881 tcgctgccgc gcccggggcg gcgtcagcca ccgccacgat ctccgcgcct gcctggcgga
     2941 agcccgccgc gtgatactgc aacatgccgc ctgctccgat gattccgact ttgatggcca
     3001 tatgatgaat tgagtgttgt gggttgatgt gtcagggccg cgatactgac gggaccacgc
     3061 gccgccgtca agacgggaaa gcacggcctg gacacgcggg tgctttttta tgcttcactg
     3121 agccaccaac aagcgcagaa agccacgccc ggccaaaggc agcctcaccg tttgagatcc
     3181 atccatgttc agcgtgatca gcgcggagtc cacttcgctc caggcgcgca aatccgcgga
     3241 gtgctggagc ctggcggccg cccactcgga ggcaatgccg cgctctttcc aggtccaccg
     3301 cagccgtgcc tgacctcccg agtgctccac tcggggagcg aacagcccgg gcgaaaacaa
     3361 acccgccgcg ccctcctcaa gcgcgaactc caccgcattg gccagaccat ccccgtccgg
     3421 gtcgtccagc aggccgccgg ccccggcggg aacctcctgc gttgccatcc attcatgaaa
     3481 cgggatgtcg ggatcaaggc cgagaatctg ggagacgatc tcgcgatccg tgagcggcgg
     3541 gatttgcgtc gtgagatatt ttgaccggaa ggcctctagg atgatctggg cgatctttcc
     3601 ctgcgcgctg gtggccggat gaaatccatc cccggagaaa acatagcggg cacgcgaatc
     3661 cgcgtcggct tgcttgtaaa attcaatgcc gccgatgcgg aagggatccg tgatcatggc
     3721 tcgtgtcagc tcatagacgc ctggcgcgaa gccaatgccc ttgctggccg cgaagctggc
     3781 aagctgggcg ttcaagctgt cgagcgcggc ggtcacgcgc gcggttttca caggatcgga
     3841 cgggtgctcc gtctgcactt tcggcgagca gccaagatga ggcaccgaga ccagcacgat
     3901 gtgcatggag gactttacgc cgcgcacata gttcacgatc cattgcagat tgtcgcgcgt
     3961 ggcattgatg tgcggggccg ctgaagcccc gttgtaaatc gagccgtagt agctgtccac
     4021 gtcgtttccg cccgcgaaga tgacgacacg ttccgcctcg tcctcaagct ggccctccag
     4081 ctccatgatc caccaaaaat tcaggatgtc tccaagctgt ccgcgtatct cctgggtggt
     4141 cgcgccaggg aaggcccagt tgtgctcatg gccggtcaga cgcgggtcgg cataggcgga
     4201 gaagcggccc aaatcgaacc agttgttccg tcgctcatgc agaatctcgg cccagtttcg
     4261 cgctgaccag gacgccgggt ttgccggata cagcgcggga aactccactt catattcctt
     4321 ggtcagactg tccccgacca ccaaacactc ggctgcaagc gcgcgctggc agagcgcggc
     4381 ggaaagcaga agcaaggcat ggcggaaaat gactggcacg gcctttcaga cggcgggagg
     4441 tcgctccttg tttaatcgcc acgcttctta atgcttcgcc agcgactcca ggtagtcgag
     4501 caggctcgcg aactccagca cggtgaagtt catcatcaga ccgggcggca tcatgctcat
     4561 gggaagcttt tggcgctctt tgatgtcggc ggttttaaag gtgaactcct gcgcggcgat
     4621 gttgcgaagc ttcacctcgt tcgcaccttc ctgggtgatg aagcccatct gtgcggtgcc
     4681 gtccttcatc gtgatcatct cgctcgcgaa gccttgggcg atggtcttgt tcggatcgag
     4741 gatggcctcc gccagctcgg gccgcttgta ggtttgggcg atgttgccga gataggggcc
     4801 tttttgggct tcggtctcct tcacggtgtg gcaggccacg caggtggctt tggtgaagat
     4861 ttgctcgccg agcgccacgt tgcctttttc cttcaaaacg gccgcgagga cctcttcggg
     4921 cttcaaggtc atgatcttgg gcgttttgtc ttcgatcttg gtgatgcgca tggccttcgc
     4981 ggcacgttcg gcggcacctt tgacctcggc atcgggatca tcgagcgcgg cgagcacttt
     5041 ggcggcgctg ggcatgtgct tggaggcgct gatggcgtcg atgatgagct tgcggtgctt
     5101 cggatcggcc cagcctttgt cgagcgcctt ctgcgtgagc tggatggact ccggactgcc
     5161 cgtcttgcgg tttgcgaggt tcagcaaggc ggcgttcgca tacaccgaca ccggccactc
     5221 gcccctctcg gcgatctgct cgacgagttg gtggtggttt tcgagcttcg gtgcgttcaa
     5281 gaaggcggcc aaggcggcat catagtcttt gccggattcg gggagcttct tgagaagctc
     5341 cagcgcagcg agtgtggcgg agacgccatc cgcgctgtcg gttttcgaca aagcgatgat
     5401 cgcttgagaa agcgtcgccg gtccgacggg tccgacctgt cgtacgagtc cgacgagcag
     5461 cggcactgcc tccacaggca cctcctccgc caccgccatc tgcgccgcca gttccggcag
     5521 caccttcggg tccttcttcg cgagttcgag gatgcgctgc tgcgcttcgt tgaactggat
     5581 gcggttgcgg ttcagctcct tcaccaaaaa cgccgcttcc tccggcgtgg ccttcgccag
     5641 cgcgtccttc agtgccacgg cgatcttcgg cgtctcgccc caggcctccg gctggtaata
     5701 cggcccccgc gtgtctggcc gcgtgcccca cgaatcgccc ttccactcac cttccttgaa
     5761 atgcaggcgg cacagcgccg cgaggatgcc ctggcgcatc gcgggatcgg tggccttgcc
     5821 gaggcgctcg atgaggccgg tgacgacttc gagagtgtga atgtgcatca gcgccaagag
     5881 cgcgcctttg cgccgagcag actgtatatt gggattgtcc acagtggcaa aattggcttc
     5941 gtgagcattc atcatcgcaa gtgctcggaa cgcaacatgg gcgattgttg gatcatcgtc
     6001 gtctaagtgg tctgtcattg gggcagacag atcacgaatc atagccttgt gctgctcagc
     6061 tttgctcatt cccaccatcg atagtccagg ccattgcaag taattcgaga gttggcgtgt
     6121 gattgacaca actgcttcga accttgttct cgagtctttt gatttgaggc cgatagcaaa
     6181 actgtttcta acatccatca tctcactgcg atcacccaaa aacatagcgt cggcaccagc
     6241 acgaaggaca aaggaagaaa gagccgagtc ggcgtttaac ttgctgatct cacgaaggtc
     6301 ttcgggccat ggaacaatag ccaatcgcaa agtcatccca aacaatgctg cgacgcgagc
     6361 cgaaaggtca ttcgacttat cggcagcaaa cgctgccaac gtttgatttg tagcggaggc
     6421 tgcttccttg cgcgagtcta tggctttgat ctgagtctct cgagttgtga ggctgaattt
     6481 ctcttctctc aaccgtgacg gagaatctct cctcaccaac tccctctgcg cctccatgcg
     6541 gcgacggttg ctcgcggact ccagcacctt caccagttcc tcatccttcg ccttcgcgaa
     6601 atccggcagc ggctcggctt tgaagccttt cggccgcacc tgcacgatgt aaccgacatc
     6661 cggtccggcc cagtcgaagg tcgcgccttt ccaactggcg caatagacgc ggctcaggcc
     6721 gtccacgtcg gcgtcggtgg ggcgggtcat tttgatgaag ggctcgggct tgcgggtttc
     6781 ttcaaacgtc gcgcctttgg gcttcacctg atgatgccag agcgcgccgc tgccccagtc
     6841 gcaggtgaaa ggggcgttgt tccaggtgcc gaagccgggc tcgtcgatgt aaaccgcgcc
     6901 gcagccgctg ccgccgccgt agtcggcgag cggctggatg cactcgtcgt tgaagttttt
     6961 gtagaggcgc gggtagccgt ggtcgtcgag gccggtgaag tggtggaagc gcacattcca
     7021 gccgccgccg tcgttcgtgt tgtcgcgggc gaagatgtcc atcagcgggc tgatggcgac
     7081 ttcgaggatg ttgcgcgtgc cggtggagta aatttcgagg ccggtgccgt ccggacgcac
     7141 gcggatgacg ccgccgccgc gatgctggag cgttttgcca tcggtgccgg tggccttcat
     7201 gaagccgaaa tcgccgcccg cgatgtaaag ccagccgtcg ataccgaggc tgaggccgtt
     7261 cgtggtgtga tcggcggggc ggtccttgta accgaaggcg atgtccttca cgaggatgtt
     7321 ctgcttctcc gcgacgccgt cgtgatcggc atcgatgaac tcgctgaggt gcggcggatg
     7381 cacgaggtaa agccggtcgt gatcccacac gagcccgcgc ggcgcatcga cctcgcaaaa
     7441 gaccttcgtc tcatccgcgc ggccatcgcc atcgagatcg cgcaatcgga tgacgcgacc
     7501 acgcttcgga ttgcggccca gcgagccatt tccgtccgac gaaacgtaaa gcgtgccatc
     7561 cggctccgcg gcgacgaaga caggataatt cgccgcagcg ctgttggcga agagggtggc
     7621 ctcgaagccg tcagcgacct tcacgtcctt gaggatggcg gcttcttgct cgggggtgag
     7681 tttcacgatg cgcgggggca cgtttccgct cttcgagtag tccgttttgt cggacgggtc
     7741 cgacaggtcc gacttgttgg acggtttggc ggtgactgct ttgatgcccg ggcctttcag
     7801 cctcacctcg cggatgctgg cccagccgcc gctctttttg ccgagcgcat gcaccttcac
     7861 gaaacggatg ccgtcggcct tggcgaagtc attcgagtag ggtgcattct tctcgttcgc
     7921 actgccatcg aagagcgtcg tccacgtttt gccatcggcg ctgccttcga ctttgaattg
     7981 atacacgccg ttgttctccc aagaggtctc gatgccggtg agcgtctgcg gcttctccag
     8041 ctccaactgc aaccactgcg gataatctgg gccgctggcg caccagcggg tgttttcgtc
     8101 attgtccacc gcccgccaag cgaagttgtt cttgcccgtc tcctcgctgc tcgcgctggc
     8161 tttgatgccg gtggcgtttt tcggcgcggg agccggtttc ggagcctcag cgggctttgc
     8221 agcgacaaat gtcactttgt tctgcccggt gaacggcacg aggtagtccg gcgtgagctt
     8281 gtcacaggcc cagagcaggc cgcgcgtgat cagatcgagg taacgcgcat cggcgacggt
     8341 ctcggtgttg tggccgatgg tcgtgctgaa gctgcgtgca ccggcttttt cattcgtcca
     8401 ggcgacgatg gcatccaccg ccttgccttt gaccagctgg cgccccatcg cgagcggatg
     8461 ggcgtcgaag acgttcacgt tgttgtagag ctcttctttg atcgtggtcc aatccgcgag
     8521 cgtcttggtg atcggatgct ctttatcgac aaaggtgatc gcgatgggct cctgcgggcc
     8581 gtgggagttc gattgcaggc cgagacgctt gaaccagcga tcctcgcctg tgcggaagct
     8641 gtgcatcgcg cagtgaagct ggatgctcgg gatggtttgg tgggcgtcga ggatgcgcgt
     8701 gagcgtctcc ttgttgttca tgctcgccgc gcactcgtcg tggatgatga tgtcgtagct
     8761 cttggcccaa tccgccttgt catagaccgg cagcggcggg ttcgtagact tgtcgtcggt
     8821 ccaaatgaca tccacccgca cattcgcgcg gctttggatg cccttggcga tgacctcgtg
     8881 ctgcttcgcg tagtcatggc agcagccgcc ggtaatgagg agggctttca gcggctcggc
     8941 ggctttgaga gatagcgcct ggagcaagat gagaaatggc agggttaggc gtagcatggc
     9001 aaatatggac ggagaagccg cgtgaaaggc gggttttcaa tcggaataac gcagacttcc
     9061 tttgactgat ggaacgcgca agccgctgct ctcgaatgca tcccaaatgc cctgaatgtt
     9121 tccggcaggc atcacttctc gagcgtccac ttcacggcat tcgccaacaa acgacgcaca
     9181 tcggcgatcc gcatgtcctc cggcgcaccg aggctggtgt agaagacgcg ggctttgttt
     9241 gcgccatagc tgcgtgtcca ggctacaggc tgcggtggag tggatgcgcc tgctcccgca
     9301 gtgccgataa ggatgggcgt ggcatcggcg gcgagcggca gcaccttgta gagcgattga
     9361 tagccgcgaa tgttcgcggt gttcacacct tcgagcagcg gtgtcttgat gccatcgatc
     9421 acgctcacag tgtaaggcag ccccttggtt tcgtagccgg tgttttcgcc gcccagcaca
     9481 tcatgcgtga actccggcca gatggtcaga ccggcatcca cggtgtcttt cgggcgcgga
     9541 atgaaagcgt ggttcgcggt gcggatgccg agcagcggtt tgccagcatt gagatgcgcg
     9601 cgcacggtct ccatctgctc ctttggcaaa aaacggcggc gcgagaagag aatgagcagg
     9661 tccgcatcct tcaacgcggc gacgaggcct tcaaagtgat gcttcaccgg cttgtcgccg
     9721 accacgacgg tcacttggtg gccctgcgga cgcagttcat tcgccgcgaa ggcgttcatc
     9781 gagttcaccg cgtcatagtt gtccggctcc tcgacggcca tgatgacgat gttggcggcg
     9841 tgggaggctg gaatgaggct gccaagcagt gcgagggcga gaagaatgtg cttcaggaag
     9901 tctgattgac gggcggttgc aaatcgccgc tcctcgaaag gggtgaaaga ctcagccgaa
     9961 caacgccttc accggctgtc cgcggtcggt gatcggcacg gggcggtcgc catcatagag
    10021 ttccttggac ggattgatgc cgagatcggc caggatggtg gcgaagaggt cgggaacgcc
    10081 gcaggggttc tcaacaggca tcttggaaag ctcatcagtc acaccccagg caccgcggtg
    10141 cttcaagccg ccaccggcaa gcaccacact aaacgcgctt ccctgatgtc cgcgtccgcc
    10201 gccgccatcg aagtcggaag gcctgccaaa ctccgtgttc accacaatga gcgtcttgtc
    10261 gagcagccca ttcgctttca aatccgtgat cagcgtgctg agcgcctgat caagatcctg
    10321 gatgagcaca tgctgcttaa gctggccctg gttgtgagtg tcccatccgg tgccgttgac
    10381 gaagttcaaa ttgtgcgaga cctcgatgaa gcgcacgcca cgctggatga gccgacgcgc
    10441 caacaagcac cgctgaccga actcgctgcc gtaggcctga cgcagctcgc ctttctccga
    10501 cttcaaatca aagacctgca tgaactccgg cccggccagc gccaggctct cctgcaacgc
    10561 ggtgtcgtat tcatcgagga tgggactgga gcgcagttcc ttcgcgcttt ggcgcatcgt
    10621 gccaagcaac tgctcccggc ggctctgacg cggatcgagg atgtgctccg gacgcttcag
    10681 accggcaggg ccggcctcgg tgtcgagcaa gtagaggaat ccggccttcg cgccgaggaa
    10741 tccaggccca cgcgagaaat tcggataacc gatgagcaca taggcgggca cgccttcggc
    10801 ggcggcgccg cgctcactgg cgatcatgga gccgagcgag ggatacaccg tggtgccgct
    10861 gacattgcgg cccgtgtgca tgcggttgga ggcagcggca tgttcatcga tcacatcatg
    10921 attcaccgtc cgcatggcgg tgatgtgctc catgagcttt gcgcagcgcg agagatgctc
    10981 ggtgaccttc acgccgggca cggaagtctc aatgctgggg tagtaactgc ctgctttctt
    11041 cgccttcgca tcacccaacg ccttcgggtc ccaggtatcc acctgcgccg ccccgccacc
    11101 gagccagatg tgaatgcaat gctccgcctt ccccatcggg aaatgcgcca catgcgcggc
    11161 ttccttggcg atcaaggcca gctcagcatt cgcggcgaca ccggcagcac cggcaagctt
    11221 gagaagggaa cgtcttttca tgggaatcga tgatggtgag gggtcacggc agatgcacga
    11281 actccggtga gttcagcacg gcccagagca ggtcttcgag acgctcgcgc cattcagggc
    11341 ggagggcggt ggtgggcgga ttgccttcgc gggcgcgttt ttcgagctcg atcttgatgc
    11401 tgttggctcc ggcggagagg tggttcgacc aggcgacata tttcagcggc ggcagcttca
    11461 caggcgacgg acgtttgcct tcggggatga tgcgctcttt gaaaccgggg ctgatcaacg
    11521 cggcaaaact gttgcgctcg gcctcggtgg gatgacgggt gagcaggcgg aggaagatct
    11581 gatccaccaa ctgggcatcg ctgagttttt catccagggc gagcgcggtg atggcgcttt
    11641 tttcatccag cgtcgtcacc cagcggccca gggcgccgtt ggcgaggatg gcgggctggc
    11701 gcacgttggc ggcggtttcg cgcacgctct tcggctcctg acgtgacgga cgccagccga
    11761 agttttccag cacgtccacg atgatctgcg ccttcggaat ggcgaggctg gggcggtcgc
    11821 gctcgttgga gagggaggtg aactcccacg cgcggcgcgg atggccgagg ctgatcatgg
    11881 cgctctcgga ttgcgtgccg tcgttgtcca tcgtcagctc ctcggagtcg agatttttgc
    11941 ccacggaggc gaagagcgag tccacaattt gttcggcagt catgcggcgc ggcagcggcg
    12001 cggcaaagtc gggcgtggct cccttttcaa gcgggcgcgg acgacgctga taagcctcgg
    12061 aggtcagaat gcggcgctcc aggtgcttca ggtcatagcc atgcgccacg aagtcgcgcg
    12121 ccagccactc cagcagctcg ggatgcgtgg gtttggaggc ctcccagtcg tccacgggct
    12181 cgacaaagcc gcgaccgaga aactgcttcc acacgcggtt cacgatgacc tgcgcgaagc
    12241 gcccgttatg gggcgaggtg atgagcgcgg cgagctgcgc gcggctgtcg ctgctcttgt
    12301 gcggtgcggc agctttttca tcggccatca gcgttttctc aaagagcgcc ggccagccgg
    12361 gcgagacttt ttcaccgggc ttgagcgtga tctcgatgag cggcttgcgt ccggcgaagg
    12421 tgctcatggg cacggaggag gtctccggca gcgtgatctc cttgcggttc agcatcgcgg
    12481 ccatttcaaa gaggtcgcgc tgctttgact gatgatacgg cgaatcatgg cagcgggcgc
    12541 aggtcatctc ctggccgagg aaagcggtgc tgaggatgtg cgccttcgcc gccatgggca
    12601 cgtcgttttg cgcggcgagc gagaagcccg ccggaccgcc cgcgagcacg ccgccttcca
    12661 tcatgatcag ctcggtaaca aaacggtcca tcggcaggtt gtcgctgagc gcctcatgaa
    12721 tccagtagcg gaaggggccg gagttgttga ggctgggctt gaggatgttc gggttctccg
    12781 ccagcacgtc ctgccagtag gcggtccagt ggtcggcgcg gcgcgggtca ttcagcaggc
    12841 ggtcaatggc acgctcgcga cgctgcggcg agtcctcggc gagaaaggcg gccacctcct
    12901 ccgcgctcgg caccacgccg acggtgtcga gcgtgacgcg acgcaggaac tcgagatcat
    12961 cggtgagcgg agcaggctcg atcttctttc ccgcgctcgc atacgacgct ccctgcgcga
    13021 tccagtcggc gagcaccttg cgctgcgcct ccgaaagcgg atcgcccttc ggcggcatga
    13081 ggtcatcgcc cgcgtcggga tgaatggcgc tgatgaggcg gctctcggcg ggcttgccgg
    13141 gaacgatggc cggatcgccg gaatcgccgc ctttttgcgc ggcttcgagc gtgttcaggc
    13201 gcagaccgcc cttcgctttc tcctcgtggc agcggtagca gttctccgcg aggatcggct
    13261 tgatctgctt ctcgaaatca atgcctcccg ccgccttcgc gctcgccgcg ttggccttag
    13321 cccaggaggc ggcgagcagc gcgtcgatgc cttccgcgtt cttcaacgca ggcttggcgg
    13381 cgatgacttt tttcgcctcg gcgtggcgtg tcgcccagaa gccgtcctcc ttcgcacgca
    13441 gggctatacg gcgctgctgg tcgagctgcg tgtagaatgc gaggcgctct ttgcgataag
    13501 cgtcccagcc ttcatccgta agcggcacgg cacgctccga gggcgtgaag agcacctgct
    13561 ggccgtcggc ggtgcgcagg ctgaggctcg tctcgccgag cgtggtgcgc acgcggccgt
    13621 tgccggcgat catttcaaag acatagacgt gcggcttgcc atcgctcttg atggtgatgc
    13681 gtgtttcgcg atccccgggc ccgagcaaac gaagcgcggg gccggtgcgc tcggcctccg
    13741 catgctccac ctcattatgc gcaccgccgc cggtgttgcc gaagaggatc tcgccaatca
    13801 ctttgccatc catccaaaga cgaccatggc cgcgtacgcg catgagcagt tcttgtttgc
    13861 ccgcaggcag cgtgacctcc gccatggcac gcagcagata cggattgctg cgggcggcgc
    13921 gcacgccggt gtccgtgtat ttttgcgcga tctggaagaa gccgaacacg tcctcttcat
    13981 acacatccac cggcgcggga tactggcgcg gccatttcgc ggccttgccg atgttttcga
    14041 cgatctccac gcgcaccttg tccttcggca ggccaccctc cggcagcacc ggcgtgtaag
    14101 gcagcacggg atagcgcgcg gcaatggttt tctccggcaa aatgccgcga tacagcctca
    14161 cctcgtccat cagtccgtcg aagctcacgc tttccttccg ccccatggag gtgccgagcc
    14221 agacctcgtc attgtccacc accggggcct gcttcgtcgc gccgcccatg tcccaagcgc
    14281 ccggcgcgag ttcgccatca atgtagccgc ggatcgtctc cggtttgcca aactcatacg
    14341 tcaccgccac atgatgccag ccgctgccag ccgcgatgcc gcccgtcgtc gtccagcgat
    14401 gccagtcgcc cgcgtgatcg ccgtctttgc ggctgcggaa gagaaagctt ggccgcaaga
    14461 atccgccgga atcccagagc cgaaggcccc agttctgatt gttcgcttcc tgccctggat
    14521 tcgatgtgcg ccctttgcca agaatgtaaa cattcccgcc cttcggagcc gaagcgggat
    14581 tcacccacgc ctcgatggtg atgctgtcgc cattgtcgaa atcgaagagg cttttttcac
    14641 ctggatcggc cacgcggatg aagctgccct tcgctccgct gaaaagcgcg gccttgttgt
    14701 ttgccgggat gtccttgaac tgcttcggcg acggcccctc ctgcccaaac accacaccgc
    14761 cctcggcctt gccgatctcc agcttgccgc catcaaagga ccaatgcgcc accggcagcg
    14821 gatccgcggc aaacgcggtg gaagcgagga tgcaaagccc agggaacgct ttcaataaag
    14881 acatggggga gcatacggcg cttgggaaag ccttttgctg ggcatcggct caaatccgct
    14941 tcggctgtag tggtaaggcg ccgacggatg cggcgaaatc ttccaacagc gatctttgag
    15001 aatcctcagc caccaaagcg aagtttcacc acctcagcga tcggcaggta ataatgatca
    15061 aggtcgcgct tgcccggggc agaaaaatga aaacagtcca gcagcagggc tttgtggccg
    15121 ttgctcatca aggtccagga cgagacgtcg cggctggcag aggccagcgg tgtgacttcc
    15181 tgcccaaagg acgagaaata gtctccaagg ttcattgtga tgacaatctc gggttggagg
    15241 atggcgactt cctcggcgat gaagttccgc gtctgcgtgg ccagacgcac cgcgtccccg
    15301 atcatcctcc agttggaagg ccaaaactcg ctctcgttgc tgaacttgga caggttcatg
    15361 aacgcgaagc tgacgccctc agccgttgca aagctgtcgc caatctggtc ggcatccggg
    15421 atttccttcc actccagcaa cccgttcaga agaccgtagg ccatgtagag catccgccgg
    15481 tggaacaggt tgcagttgag atgctgggaa ccgatccgct tccctgtgcg gtaggccgcg
    15541 tgaagcaggt ccaggtaatt gaacccctca atctcccgcg cctcgcggcc aatgaaaaga
    15601 atccgcgtct tccggctgaa gtagtgcgga taaaacccat caaacaccat gccctcgatc
    15661 tgctccttca tttcaggagc cgcctcaacg gaggcgtgca gccagtcctg aaaaagctgt
    15721 cgcagcgctt caagcttcgg tatttcggag gctgggtagg cgagggtggt ggggaggagg
    15781 ggcatgggtg tgtggaatat gggttacagt gctggcgaat gcttgccttt gttgggtccg
    15841 gatggatggc ggatgcagaa tcccgcggtg aggctgctga tggatgcctg ggatgtgccg
    15901 cagtacttgc atgggtattt ggacttttca ctgccctcgt aaagctcatg ccggcccttg
    15961 tttggaccca agggatggcg aatgcagaat cctcctgtga gactttggag gctcgaatat
    16021 ttggttccgc agtttttgca gtagtagctc atggtgtggg tttgttggtc gttatcgtgt
    16081 caaacagttg gattctggtt cgtgcagggg cggccaggac gggtggctgg agttggctgt
    16141 gaagtcagca gcgccagcat cacctccccg ccgcgatggc atccgactgg gcgtggtggc
    16201 ggttcagctt gatgccgagg tgggcggctt tgacaaaacc ggattctctt catcgtagat
    16261 caaacagata aagcagcgct gcataaccgc tgggcagggc ggcaggctga gcgtccaact
    16321 cccgcccata cttgcaggcc agcttcacaa agtagtaagc atgaaacaac gcctcgacca
    16381 tcggacgtgt gtgacgcggc caatccccgt tgtgcgacag ctcagataga ccccgtcctc
    16441 gatttcccag tgactctcca tcttttcgag gagcgcgttc aaatcgtctc tcttgtcctt
    16501 tagtcggacg agaaggcggt cgcgcaatgc atcttcagct gatgtggttc ttgaataatc
    16561 attgttatgg ttttcacatg gggtctgggg tagcatcttg tactggaatt atgttatcaa
    16621 atagcctgtt ctgtgtaata attaacagaa tcatttcttg tgctgatcaa gcgctcaata
    16681 aatttacaga caaaattggt tgagatgtcc ttgaaggacg actggaacag atgaggccac
    16741 tcctcattcg ggatagcttg ttgttcagga actttcagaa acacgacgtg aaacggaatc
    16801 gttcgccccc cttgaatgat cgcatcgctg agtatgccag atgtcgcctc aaggccgcta
    16861 tcatgccagc gagactcacg gatcgggtag atgaagccac atccggcaac tcggtgtcgg
    16921 ttagaaacct gcccgatata agtatgaagt tgaaaaaggt cttcacggct gactccacct
    16981 ccgtttctac cggagaactt gaacgacttg tatttcacgt cgaacacgta ggtcgcgccg
    17041 ttgatgtcga agaggagatc aggaatgagg cttcggcgcg tgtagccatt caggaggcca
    17101 gacggaatcg ttaattggcg gtcattcttg ctctgaaaaa ccacgcccgc ccgcttgagc
    17161 agcttgcgaa tgaaaaactc gaacagcatc gagacatcga aaaagaaggc actggtcttc
    17221 gatttgtcgc cgaagtccga caactcgttc cgcagaattt gcttcgagag cgcgatcacc
    17281 gggttgtaat ccgcgtaata cggattgcgc accggttgcg tagccagcag ctttttgagc
    17341 gcatggtggc gtccctccgt ggcgatctgg aaagtctgat tgagagtgtg cgcgccgcga
    17401 aggaagtcgt gggaatcaag atgttcgagc gtgcgggcga tcagccgcgt ggcgtcgttg
    17461 tcgtagctgt gctcgcggta tgtgcagcgg tagcgggcga gcttctgatt caggaaataa
    17521 tcaacgggat cgagacggcc gcgcacttgt gtgaggcttt ccgtgctcgt ctcgtaagcc
    17581 tttggtagcc cgagacggaa ggcttttttc agcttcacaa gccagaggta gttcagcagc
    17641 cattcatatc cgccgcgctc cgtgccgcca tgctccggaa gctccgcgaa gccatccgca
    17701 tctgcgataa tgtatttgag aaactcgtcg ccgaatcttg aggagacgct gagcgcgcag
    17761 ccatcgctct tcacgcaccc gatgaggttg cccgtggtca gggtgaagtt aagcggatcg
    17821 aagccgctca gcgtgacgaa ggcatcctcg gtattatgtt cgtattccgc ctcgggcacg
    17881 cggaagacaa gtctcgactc ttgagtgttc aatgcggccg cgcttcgcaa gacggaccac
    17941 ccgctcaacg ggtgggtcca tcctttgcct cgctgcctga tattcaccgg ctcgccattc
    18001 cttcctgcgc gaaagacccc gtcctgcgct gtgtggaccg tgttgtccac cagccagccg
    18061 tcgctcagga tttcagtcag cggcgtgtcc atcagaagcc aaatgctttt tgaaactccg
    18121 ggatgagcgt ctccgcacgg ccggaaccac gcagatactc ctccagcagc ggccggatgg
    18181 aatcgcccca gacgctccgc cgcacctcgg tatgcgtcag cgtgggaggg tagcgcaggt
    18241 tcatcaggta ggcgtgaccg atctcgtaat ctgccccgag aatctcctcc ttccgaatgc
    18301 gttcattgag ctgagccaaa tcgtcagcga gccccgccca cggatgcgat ccattgcctg
    18361 gttcggcatc acgcttctgt aggtgaagtc tcacggcgtc gatatctggg tcagcccgct
    18421 cccagcggaa gcgccgacgt aacgccaggt cgaagctctc cacgcttcga tcgatcgtgt
    18481 tcatcgttcc aatgatatag acgttgtgcg ggatgaagaa ccagaactcg gcgcccgtcg
    18541 tcagcatcgc cgtctccttc gtatttaatt gcgcgtattg cgtggagatg cgccctttga
    18601 cccctcgata ttcaattgcg aacataagct cgcccaatac gcgcgaaagc tccgctctgt
    18661 tgatctcgtc gatgatgaag aaaaacggcg gcaccgcgtc ggcgactttc tccgaatcgc
    18721 ccagcgtccc gatgccatcc cacgaagggt gttttagttc cttgccgatg tgcggcttta
    18781 aatcgcccac cgtcagcccc tgccagtcgg ctgcaagctg cgggccggcg ctcggaatgc
    18841 cgtggacttc gatctcccac gttccggcgc gacgacaaaa ctccttaaag actccattct
    18901 ggagcgcaag ttgaggctgc ccgtttctca actccggtcg cagcccctca atgaaatcct
    18961 catagccgta ggaggggtga aattgcacca ccttaatgca ctgaaggtga gtcagttcgg
    19021 gtgcgatatt tggatattta tctcgccaaa tatcgaagaa cagctttgtg ctctgcattg
    19081 ctgtgtaagt cttcccggtg ccgggtgccc catatttgat cacctgcttc ttgagcgtga
    19141 aggggttcga gatgttctcg atgttctcga agagatacca gacaaacatg ctcaggagct
    19201 gctcgctggt ctcgcctatc tccagctcct ccgcaaaggc tgcgcgcagc atctccatca
    19261 cctgcacgtt tcgatcatac caccctgcat tgggatcgga gaggcgctcg aggatgccct
    19321 ccccgaccag ccaccggtaa actctgtcga aatccggaac attcaccgtg ctcgtcactt
    19381 tagtcgtgca agccgcaacg gtgcgattga tcagcagcgg gttcgccttg gctctgtgct
    19441 tctgtcgcgt cgcctcccat acttcccagt atttcgagaa attttcgcgg ctcgggtcgc
    19501 ggatcagttc cgccagcgcg gctacgaaat tcggttccgc gatgaatagc tgaaagttat
    19561 cgtcgctcag gaccgactgg cctcgagagg aaatgccgtt ggccttctta tacagcagat
    19621 gctccgcaaa cgcagcccat ggatcactag tgagctcaaa cctagttccg gcataagtgg
    19681 acttgtcgcg gatgtccaac tgcagcttca gtgaggcgcg aaccttttcc acctcactgg
    19741 tgaattcaat ataatccgcc agccaccccc gatccacttc ggcggcattg ttggtaatga
    19801 gataggtgta gaggttccgt aggcgtgaca tgatatttag attcaaactt gtgacaacat
    19861 tgatggcact tcctcatact gacggcaatc accttgatga ccgaaccgca caggctccat
    19921 gcctgtcttt tgcaagagtt tattcgccgt gtttgcgttc gaatgggttg atcatttcca
    19981 gcatcaccct ccccgcagca atagcatccg actgggcgtg gtggtggttc agttcgatgc
    20041 cgaggtgggc ggcgagggtg ctgagctggt ggttggggag gtggggccag gtttggcggg
    20101 cgaggtgcag agtgcattcg tgccggaagg gtggcgcggg gatttcaaaa tgctccagca
    20161 gggcgtgcag gacgcggagg tcgaagctgg cgttgtgggc gacgacaagg tcggcgctgg
    20221 cgaggtgggg gatggcttcg ggggcgatgc cgccgaactc gggctggtcg cgcacgtcga
    20281 accagctgat gccatggatg tcctcggtcc attcttcttt gaaccagccg tggcccttgg
    20341 ggggcttgat gagccagtaa ggggactcaa tgagttggaa agacagtggc tgcggctttg
    20401 ggggagtctc ttttatgggg aacaggcgtc ccgcctgttc gcacaggcaa gatgcctgtg
    20461 ccccatgctc tgacgtgctg caaaagccgc aaccactttc aaagatggcg aggccaaggg
    20521 cacaggcgct caaaggactg tgattggcgg tttcaaaatc aagggcgacg attttcatga
    20581 gtaatcgggg cattcctgcc ccgtgttatg aatgaagggc caggaatggc cctgttacgg
    20641 atgtatgcgt ggcgggccag gaatggcccg gttacgcggt gaagccaatg gctcgccggg
    20701 gttggggtgc gggtggttca tcggcgtaga tttcggcaag ggagtagtca ccgtggccgg
    20761 gtagggtttt gccgagatgc gcggcgagac tggcggcggc tgcgtgcggc aggcggtcaa
    20821 acaaacgatg ggtgagcagg cgtccgggcc gcagcagggc ggggtcaatg tccgaggcgc
    20881 ggcagttgat ggtgcagatg atctggagac ggaggaagtc ggccagcatg ccgtcggaga
    20941 gattcaggat ggcgctgacc tggtcccggt tgtcgggtcc gcgcgcgagg atggcggtgt
    21001 cggagtcttc caggatgacg acgaagtttt ccttgtcatg gcggcggcgc tcgtcggccc
    21061 agaagccgac aaactcgggg tgagtgagca cggaaaggga gccgttcggc acaaagtaaa
    21121 agcgatgagt ctctgtgagc gcgcccatga gatggcggag gtaggaggtc ttgcccgtgc
    21181 ctggcggccc ttccagcaag gagagtccat ggcgatgacg ccgcagcagg ccgaggtact
    21241 gctggtgcca ttccacaaag ccggggccgt agtgaaggct aaggcgcgcg tcatccagaa
    21301 cggtgtctgg tgacagcggg acgttctgcg tgctgatgtc gcgcccggtg cggatgaggc
    21361 ggtatctgcc gccgcgcggc acgggaggct ggaggccatc ctcatgaaac tgccgggcga
    21421 gctgctcagc ggtctgcgct gtgtccgcgc aggcgatgat gcggctttca tccgcataga
    21481 caaaggcgtg ggagcccatg cggaaggtga gatcgtccca gaagagggtt tcctccctgg
    21541 aatggaagcg ctggatggaa cggaacaggt gtctggaatg gatggaaatg agtttttgca
    21601 gatcgtagct gccatccatg cggcattcat gcatggggcc tccgccaaag gcagccagca
    21661 cgggaccatg catccggccc aggtgcgggc tgaaggctcc ggcctcggcc atttcacaaa
    21721 cgagctgggc cgggcgtgtt tcgggcacgg catgcgatgt gtgattcggt tcgtcttcgg
    21781 caagtctggc ttgaggcaaa ttcatgccga gatgctgcca gcggccgggc tggaaaaaag
    21841 ggatgcgatt gtccccattc cttgtcctca atcctggagc attttgcgca ggcgttgcag
    21901 ccgctttgca tcggtgtcct ccacccagcc gagaccctgg cagagaaaac ggtgcctggc
    21961 cgcgccgcgc aactttttgg acttcgcctc ggcgtcgagc atgcgcagct tggcggcctg
    22021 ggccatgcgt tccgccagcc cctcggccag cgccgcgccc tgcctgccct tgacgcggag
    22081 gcggtgcagt ttgttgatgg catcccagcg catgtcccgc ttgggatcaa aggaccagaa
    22141 ggaggggatg aggatcatgg tgccgtgcgg cgttacattg acggccagct tcagcagcag
    22201 cgggtcatca tgcatcatgc catagaggtt ccagcgcagg cagaaggcgt caaaggccag
    22261 acgaaacgcc tctccagggc ggatgagccg cgcgcgaaat tccggccgca tgttgcgctc
    22321 cgcgcccatg gtgcggcgaa gcacgccctt gtagtcctga taagcactga catcgaaggc
    22381 cgctttgatg cgcagccagt ctttttgaaa ggcggggtcc gccttcaggg ccagctcagc
    22441 ctgggcaaac ttggcctggg tgttcaccac gtcctcataa ttgccgc
//
LOCUS       DAOMYJ010000070        22437 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Prosthecobacter sp. isolate AMR_MDS_5629
            NODE_834_length_22437_cov_22.022965, whole genome shotgun sequence.
ACCESSION   DAOMYJ010000070 DAOMYJ010000000
VERSION     DAOMYJ010000070.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867850
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prosthecobacter sp. (wastewater metagenome)
  ORGANISM  Prosthecobacter sp.
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Verrucomicrobiaceae; Prosthecobacter.
REFERENCE   1  (bases 1 to 22437)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 22437)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.30
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:34:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,742
            CDSs (total)                      :: 4,684
            Genes (coding)                    :: 4,676
            CDSs (with protein)               :: 4,676
            Genes (RNA)                       :: 58
            tRNAs                             :: 55
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22437
                     /organism="Prosthecobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_834_length_22437_cov_22.022965"
                     /isolate="AMR_MDS_5629"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1965333"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..189
                     /locus_tag="PLP58_09365"
     CDS             <1..189
                     /locus_tag="PLP58_09365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ08765.1"
                     /translation="ARAAAAPAETVEDILGSLSLRALLAACVLCAASAAYACADMLDT
                     RLERPAFEQTLAEELSWP"
     gene            186..560
                     /locus_tag="PLP58_09370"
     CDS             186..560
                     /locus_tag="PLP58_09370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ08766.1"
                     /translation="MIFRRLRLRVLLLLALFGAVSAGVGFFLGIAAAKGAQKKKDDPA
                     VWRQAALRRLEGLRPDEAQKPRLEARVDQAVKDLADLRVEGIRRVWEVVDGAVHDIEA
                     ELTPEQREAFEKMKPRPPKEAR"
     gene            607..1266
                     /locus_tag="PLP58_09375"
     CDS             607..1266
                     /locus_tag="PLP58_09375"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HRJ08767.1"
                     /translation="MKKPKPRVCAEIAPGLVLDPRRALVHRAQGWMAVADVHYGYERN
                     RRRQGALLPDWGMAACAETLRALLDEHQPRRLILAGDIMDGAGSAEETADFLEQLRPL
                     AELVCLYGNHDRAGLRKRLNLARAHREDGFIFHHGHESVEAEAGLIHITGHEHPAVRL
                     GDGAGLRLKLPALVRERLPDGGERWILPAFSPWAAGGEYHSSCGRIATWVCAPGRVWR
                     L"
     gene            1359..2291
                     /gene="fabD"
                     /locus_tag="PLP58_09380"
     CDS             1359..2291
                     /gene="fabD"
                     /locus_tag="PLP58_09380"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012420543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HRJ08768.1"
                     /translation="MPVSAVLLFSGQGAQKVGMGQDLAAAFPGARALFEQADAALGFP
                     LSRVMFEGPMEELTKTSRCQPALYTHGLALLGALRERAPGLEVTAAAGLSLGEFTAHA
                     AAGTFDFATGLNLVFQRGSYMEQACEAARGAMVAMIGGEEEAVRALAAECDVDVANLN
                     APGQIVLSGCVEGIEKAAALAKEKGIKRAIPLPVAGAYHSRLMRSAQEKLAAALESAA
                     ITPPGVPVVSNFEARPVTDAADIRATLTAQVTGSVRWVESMRHLIAAGHRLFIELGPD
                     TALAGMLGKIDREAKVISVKDTATLDAAVAELGS"
     gene            complement(2349..3539)
                     /locus_tag="PLP58_09385"
     CDS             complement(2349..3539)
                     /locus_tag="PLP58_09385"
                     /inference="COORDINATES: protein motif:HMM:NF012401.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HRJ08769.1"
                     /translation="MSPISRRQAVKLGIGSLAAMQTSMHAEEPAQIKPRSFLTPADDF
                     EDVSRGTPKPHSLTGEALVKAKLTPESWKLEISADETTNEVVTQKAQIGKAVTLDYAQ
                     ILDLGKKHRVLFLKAMQCLNIATPLGQGLWEGVPLREVLRLCGAMSNVRRVYFWGFHN
                     DDPKQRFQSSLSYTQVMETPPGELTVFIAYKLNGEPLNLERGGPVRMVVPWAHGFKSI
                     KWLQHIRLTNDYQANDTYAEKNNDPESHLKTAAYLDDVVVSGKTAHIGGWAISGLSGL
                     SHVETWLRPAGPVLADNDPEWQKAVWQPAKLDAQPDWKSALPAGTRPQDILGFDKTSG
                     KPLSWPLRYSMVGFSTTLRDLKPGKYEIRARTVDLNGFAQPEPRSIQKSGKNGIQVRV
                     FTIT"
     gene            complement(3605..5077)
                     /locus_tag="PLP58_09390"
     CDS             complement(3605..5077)
                     /locus_tag="PLP58_09390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018970898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arylsulfatase"
                     /protein_id="HRJ08770.1"
                     /translation="MKHLWLILFLCSSSFAAEQRPNIVFLICDDLGYGDVHCLNPEHG
                     KIATPGADRLASQGMIFTDAHSGSSVCSPTRYGVMTGRYSWRTKLQSGVVEGFAPSLI
                     AKDRPTVANFLKSAGYHTAIIGKWHLNFEYLDPKSGQPYKAKEFDTPPVGAKIPDGPI
                     TRGFDYYHGFHHARDMEAVIENDEVIEHDEVVHMLPRLRRKAVEYIDSRKGREQPFFL
                     YLPLGSPHTPIVPAPEWQGRSGLGDYGDFVMQTDDVVVAVSEALERNSMKQNTLFILT
                     SDNGCSKAAGIDKLAEQGHKVSANLRGSKADIWDGGHRIPFIARWPAKIAAGSTNDQL
                     ICLVDFFATAAEILGQPVPAGSCEDSVSILPAFNGEKIVSTRHGVIHHSISGHFAYRQ
                     GPWKLALARASGGWSSPTEAKAPANWPKGQLYNMKDDIGETTNLFESKPEIVNQLLDL
                     LQANISRGRSTDGPDSTNDFADIVLWKSEKGKKTGKKKDK"
     gene            5166..5480
                     /locus_tag="PLP58_09395"
     CDS             5166..5480
                     /locus_tag="PLP58_09395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ08771.1"
                     /translation="MTITHMSQDGIEIHFDEKHGHLRLACEPDAFAAYQSVAREHLKD
                     FPDIPVDKVIEMNIVDTATFVARRDAPKRRIGGLFFGIIVVVIIALAVIGAFALFTKI
                     TA"
     gene            5568..6071
                     /locus_tag="PLP58_09400"
     CDS             5568..6071
                     /locus_tag="PLP58_09400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ08772.1"
                     /translation="MNERDTVQPLAEFPQSSVGAPTPMILASEQALFVAFYLEQRDPN
                     WDGTTCKMVTPDTNDEPVAVVRFSGVTAHFFGPPNDEAFSGHPLAERGLEPYGAFEIL
                     SSSWIRALERMNSVHPRHHPDNFSSCRHFVLTFHDTIFECVADSFTIERPSGSLRSVA
                     SSLAAAL"
     gene            complement(6144..6314)
                     /locus_tag="PLP58_09405"
     CDS             complement(6144..6314)
                     /locus_tag="PLP58_09405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ08773.1"
                     /translation="MCPSSDATAAKAKLQGALDQFKDARPAALDQQFLNSGAGKDKPE
                     KKGKKKNKKKAE"
     gene            6354..6761
                     /locus_tag="PLP58_09410"
     CDS             6354..6761
                     /locus_tag="PLP58_09410"
                     /inference="COORDINATES: protein motif:HMM:NF024861.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - endoribonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative toxin-antitoxin system toxin component,
                     PIN family"
                     /protein_id="HRJ08774.1"
                     /translation="MFAVLDTCVLVSALRSSSGASHAVLQAVYARRIRIALSVSLAME
                     YEAVCSRPGLVPALTAGQIRTVVDVLCALAHQQKVFFTWRPHLPDADDDHVFELAVAA
                     GAPYIITQNFRDFRGSDSLGVQTITPARALTLI"
     gene            6764..7003
                     /locus_tag="PLP58_09415"
     CDS             6764..7003
                     /locus_tag="PLP58_09415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009958648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlcI/YnfO family protein"
                     /protein_id="HRJ08775.1"
                     /translation="MSTMSIRIPDSLHRGIKELATKDGYTMNQFIITAAAEKLAALST
                     VDYLGERAKRADFKEFERIMALIPAGPPDPGDELP"
     gene            complement(7038..8456)
                     /locus_tag="PLP58_09420"
     CDS             complement(7038..8456)
                     /locus_tag="PLP58_09420"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HRJ08776.1"
                     /translation="MKALLFLLLSSFAIASLRSLLRSSTRSAVLAVSLCSALVSSFAA
                     EQRPNVILIMVDDFGYECVTANGGESYQTPNLDKLAASGVRFENCHAQPLCTPTRVQL
                     MTGRYNVRNYLNFGTLRREETTFGHLMKNAGYATAICGKWQLGHELDSPQHFGFEESC
                     LWQQTRRPPRYTNPGLEYNGVEKDFTKGEYGPTLVNDFALGFIEKNKGKPFFLYYPMI
                     LTHNPFQPTPDSANYDPTISSEQKLQNPKHFADMTAYMDKMVGRIVSKLDELGLRENT
                     LILFLGDNGTNSGLTSRFQGRDYKGGKGSTNAHGTHVPCIASWPAVMKKGRVSADLVS
                     CADFLPTLCAATGVEAPAGVDGVSFLPQLKGETGTPREWLYIWYSPRQSKDMTVREYA
                     FDQRFKLYRTGQFYDLNADEMEKQDLAATALASDAASAKAKLQGALDQFKDARPAELD
                     QQFLNSGAGKDKPEKKGKKRRT"
     gene            complement(8453..10015)
                     /locus_tag="PLP58_09425"
     CDS             complement(8453..10015)
                     /locus_tag="PLP58_09425"
                     /inference="COORDINATES: protein motif:HMM:NF019474.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HRJ08777.1"
                     /translation="MTPQAIRSRVFLIPLLFFTSLRAADELPEPTISWKYPPEMTGSR
                     VEVYRTIGDVKLNAHIFEPEDHAKTDHRPAAVFFFGGGWKGGTPGQFLPQCLHLAKRG
                     MVAISVDYRVKSRHNVFPQDCVRDAKAAIRWVRANAERLGVDPDRIVAGGGSAGGHLA
                     AATALLPGFEDDNNKGVSSVPNAMLLFNPAVVLAPVDGHPDLLTAEKFADIRERCDGR
                     PQEVSPYHFVRAGLPPSIIFHGTNDEAVSFPTVQLFQKVMSAAGNRCELKAYEGQPHG
                     FFNPGRWKDAAGARAKLHYYKTLAEVDAFLESLDYLTPAPIVETFDAGFDAKRFTTPI
                     PNKNTEVRDGVLWTRGRSGGKYPPMVYLPVSGKDLAISFRYRHLGQGGMLWFFVDGDD
                     GFGSVDHMLRVKLLRDGVQLQVDSHSLDPNHPLRQKQERPADKVSGAYRLNEFLPLER
                     ADLGANAWHTVKLVFNGESVAMSVDDKLWSKTLSRANFNAGKRKLLWMQNGGEKGIEI
                     DDIHVEPSAPMP"
     gene            complement(10012..10971)
                     /locus_tag="PLP58_09430"
     CDS             complement(10012..10971)
                     /locus_tag="PLP58_09430"
                     /inference="COORDINATES: protein motif:HMM:NF012363.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HRJ08778.1"
                     /translation="MKPALITLLLCLPALALSAADNPKAKAPAKKKAAPANPAVVKPT
                     MANVPYGTHERHILDFWKAESATPTPLVFVIHGGGWQSGEKERVNKFVSVEDLLAQGI
                     SVVAINYRLMKHAAEDKVTPPVKAPLHDAARALQFVRSKAAEWNIDKTRVGAAGGSAG
                     ACSSLWLAFHDDLADPASSDPVARESSRLQCAAVTGAQTTLDPQQMKEWTPNSRYGGH
                     AFGIGSFAEFLAGREKILPWIAEYSPYALVTAADPPVYLIYSAPPALGQDQKDPTHTS
                     NFGVKLQEHCVATKVPCELVYPGATDVKHTTPTAFFIATLKAK"
     gene            11231..12427
                     /locus_tag="PLP58_09435"
     CDS             11231..12427
                     /locus_tag="PLP58_09435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009958091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1552 domain-containing protein"
                     /protein_id="HRJ08779.1"
                     /translation="MAKSVGGSVQAAKGAGMGAKRFVAIGNLLGYQTKSLFPTTQGRN
                     YEKTTLLEPVWDVRDKMTVLRGLDHGVKGGHFAVHSFLSGVLHSEAQNRPDGNVTIDQ
                     YLADAVGFETRFPSLTVGSEGGIHGGCQIAWTKSGVRVPPVTNPAELFEKLFISDSPE
                     RQARRAQENKVQASILDSVLDEANRLSKKVNQEDKAKLDEYLTSVRDVEKRLELRQRW
                     TNQPKPKAPFDKPANRNAVEDIPLLYELIALALQTDSTRIATLEIGGDFMPQHLGIKK
                     DWHGLSHHGNDPEAIESLITLEKYQIEHFGKFVARLAKMNDGERTLLDSTTVMFGSGM
                     GDGNSHKNSDLPILLAGGGYKHGEFREVPREGINKVPLCNLFVDIAQKMGVETDSFGS
                     STGRFA"
     gene            12632..15028
                     /locus_tag="PLP58_09440"
     CDS             12632..15028
                     /locus_tag="PLP58_09440"
                     /inference="COORDINATES: protein motif:HMM:NF019259.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1592 domain-containing protein"
                     /protein_id="HRJ08780.1"
                     /translation="MQQFLGKYCLECHDADVQKGDRSFEKFELPLKSVPDLIEARDII
                     DQLTLKEMPPKKADQPKDDERIAMIRALRDGTVVAYGKFQSTGSRTVMRRLSSREYEN
                     TLAVLFGRRVDTLGLTAGFPKEKTTEHMDTIGKSLVTSGFLVDQYFQSANRLVETRLG
                     KPATPPKDWKFNGHFVQYEELSGSHKSAFNYRYLNIYEQPNSDTRQGGYGHIEDFKQG
                     VPVSGLYDLEVEATAMHRDTHYDPAIFGIDFSEPFILGVVPGDVTAGHIHYPQPIEPV
                     LATAVVPDNEPKRLKFRVWLEAGQTPRFIFPNGPYESRAAVVTINKRYKDEFKGDVGS
                     SGVSRAHILKEGKLPHIRISEIAIHGPVPEAPGAEEVAVFGKEGFQEAKALDQLNAFA
                     EKAYRRPITEEDKKPIRALYEKRIAEKVAPRQAALDALKLILCSPSFLYLSEITDESE
                     KHLKPYDLATRLSYALWSAPPDDALLAAAKSGKLTEDAELKKHIQRMIADDRINGFVN
                     GFLDSWLNLRDLGSQPPPREVVRSYYAENLPTSMKMEARLFFRDLLKNNGSVTQFIDC
                     EHTFVDKKLAKLYELPEKDKLRLADGFQKVSLKGNNHRGGLLGMAAVLTVSANGVETS
                     PVTRGVWVTENFLGVPPPPPPDVVPAIDPDVSGATTIRDRLAKHRADAACAECHRKID
                     PLGFSLEAYDPVGRWRKKYPAANKKAKPAEIDTTGEFPSGETYADFTGFKRVIRDTRS
                     DLFSRHLIRQLLTYNTGRTMELADDLPLDQLHEKVKQQGLGLNTIMVECLMSEVFRSR
                     "
     gene            15039..15485
                     /locus_tag="PLP58_09445"
     CDS             15039..15485
                     /locus_tag="PLP58_09445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ08781.1"
                     /translation="MKEAAYDYLKLLEEHDHTRRCEYPPSFDLKAAEERFTHLAEELI
                     AAYQGSTFETGAAIQDASFHGQIFLAFEGLTILIRASNFGRFITFFDEEEHMLLPDET
                     RCWLLELFIKHGYRFIPPEVLSMPYTGECKGVSGFRDWGYRYFEWI"
     gene            complement(15467..15664)
                     /locus_tag="PLP58_09450"
     CDS             complement(15467..15664)
                     /locus_tag="PLP58_09450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ08782.1"
                     /translation="MTDLMRQLTARKVERRRQLAALPVGEKLRMLEEMVAATKAISAT
                     RPPKPVNPVQFSRQEIQIHSK"
     gene            complement(15661..16176)
                     /locus_tag="PLP58_09455"
     CDS             complement(15661..16176)
                     /locus_tag="PLP58_09455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ08783.1"
                     /translation="MDKIFAVLNQMESDGVIGRYAIGGAVGSIFWLEPITTKDLDVFV
                     MLPTAPGGSLLTLGPIYEYLLARGYSPQGQFIIIEGWAVEFVPPGTPLVEEALAQAVA
                     RDVNGVSTRVFTAEHLAAICLQVGRPKDHDRVIRFVEAGVLDAELFEPILRRHDLWEK
                     WQKFQQTYLEP"
     gene            complement(16229..17548)
                     /locus_tag="PLP58_09460"
     CDS             complement(16229..17548)
                     /locus_tag="PLP58_09460"
                     /inference="COORDINATES: protein motif:HMM:NF024571.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HRJ08784.1"
                     /translation="MTAFQTAALQEKLALGTSQELPVLTHRQVPMTLLPGKAQAVIGM
                     RRAGKTCFLFQQMQERLNQGVPRERLVYLNFEDERLPGLEADALTFVLDEYFRTHPGV
                     RGREKVMLCLDEIQLVKGWESFVRRILDSEKVEVFLSGSSAKMLSQEVATAMRGRALE
                     VIIHPFSFRELLAHQKVPLPEDVTMLTPAKRSRIEKALADYLSGGGFPESLGLPLDAR
                     SRLLQSYVDVAMLRDVVERHQVANVAALRFLVRHLLANAGSPFSVQKIHNFLTTQGMP
                     VAKNTLHDLLAHLEDAFLVRTVWMEASSERQRMVNPRKAYPIDTALIALYDRTGRANP
                     GHALETLVLLELERRHSTATWVRTPAGYEIDFLARHANGRHELIQVCADASDPETAER
                     ELRALADAAAVFPKATCTLITQHQNGFPKRPLPAKTRVVTAWQWLLE"
     gene            17646..18365
                     /locus_tag="PLP58_09465"
     CDS             17646..18365
                     /locus_tag="PLP58_09465"
                     /inference="COORDINATES: protein motif:HMM:NF018178.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1080 domain-containing protein"
                     /protein_id="HRJ08785.1"
                     /translation="MIRFLLLSAIICIATLRAGEAGFVSIFNGKDLSGWTTREAIKGD
                     WKVVDGAIDCDPQGEGKGDRNLWTIKEYGDFELFIDWRIKETPFTNPKERVILPDGSH
                     QKDPSGELATIAVPNTDSGVFLRGQHKSQVNIWCWPVGSGEVWGYRTDARFSAEVNAG
                     ATPKKKADRPVGEWNTFHIVMKGDRLTVTLNGVEIITQAQLPGIPEKGPLALQLHPER
                     KDGEWGASLVQFRNIRIKELK"
     gene            complement(18638..20191)
                     /locus_tag="PLP58_09470"
     CDS             complement(18638..20191)
                     /locus_tag="PLP58_09470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009964424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative Na+/H+ antiporter"
                     /protein_id="HRJ08786.1"
                     /translation="MTPRLLLASLVLLACPLAAASLEDKPPFEGHAQLAHFPRPLESY
                     GDRGQESARVKIMSRAAQEPFNVAATIIFGLAVLHTFASGFFRKWAHHIEHVHHEKIE
                     RERRTAKDKPHEDAEDDVSFGATILHFLGEVEVVFGIWVLALMAAGVYFHSWHDVQLY
                     LGHDVNYAEPLFVVVIMAIAASRPVIQFAEKCVAAVASLGGGKVGAWWLAVLTLGPLL
                     GSFITEPAAMTISAMILARKIYPLRPGKSLAYGTLGLLFVNVSIGGTLTHFAAPPILM
                     VASPWKWDTAFVFSTIGWKCAISIIIGNALFYFWFRNELAELDHRAHPQEGESRLLRW
                     ADREDPVPRRVILVHTAFLAWTVLTNHYPAMFMGGFLFFIAFVEATRHHQNLIGLRSP
                     ILVGFFLGALVIHGRCQSWWIEPILTSGVPDWVLLLGSAFLTAFNDNAAITYLASQAP
                     GLTDMAKYAVVAGAVAGGGLTVIANAPNPAGLSILSRFFQGGVSALFLALGAAVPTAV
                     VITTLLVMK"
     gene            20410..21405
                     /locus_tag="PLP58_09475"
     CDS             20410..21405
                     /locus_tag="PLP58_09475"
                     /EC_number="1.2.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007412604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HRJ08787.1"
                     /translation="MPRRITYRQALREAFDEELARDPMVVLLGEEVAQYNGAYKVTEG
                     LWEKWGDKRIVDTPISEAGFIGMGVGASMLGVRPVMELMFWSFYTVAWDQIINNAGMV
                     RYMSGGKIHCPIVVRGPANGGTSVGATHSHTPENIMASFPGMKCVVPSTAYDVKGLMK
                     AAIRDNDPVMFMESTKLYGEEWEVPSNDELPEGELVIPLGVADVKREGADISLICHGK
                     AVLTCLEAARILEEEHGISAEVVDLRSIRPLDEETVLNSVAKTHRAIYVEEGKPFCGV
                     GAQIAYMIQERIFDELDAPVLRVCSLDSPAIYSPPLEALQLPTADVVVEKVLSIC"
     gene            21415..21642
                     /locus_tag="PLP58_09480"
     CDS             21415..21642
                     /locus_tag="PLP58_09480"
                     /inference="COORDINATES: protein motif:HMM:NF016167.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF433 domain-containing protein"
                     /protein_id="HRJ08788.1"
                     /translation="MDYQQHITIDPQIRRGKPCIKGTRIAVADVFDYLAGGSSKEELL
                     EDFPDLTEADVMACLAFAADRERRYAVAMAG"
     gene            21684..22016
                     /locus_tag="PLP58_09485"
     CDS             21684..22016
                     /locus_tag="PLP58_09485"
                     /inference="COORDINATES: protein motif:HMM:NF037832.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5615 family PIN-like protein"
                     /protein_id="HRJ08789.1"
                     /translation="MKLLLDENISHKIAGLIQPWFPGSQHVRDAGLKGRPDHEIWSFA
                     AANGFAILSKDGDFEAISLLRGQPPKFVWIRIGNCTRETLLQLILAHHEDLLSFDADP
                     VESMLVIS"
     gene            22068..>22437
                     /locus_tag="PLP58_09490"
     CDS             22068..>22437
                     /locus_tag="PLP58_09490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009957898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoamide acetyltransferase"
                     /protein_id="HRJ08790.1"
                     /translation="MPKIIEMPKLSDTMTEGTLAKWIVKEGDTVTNGQAVADVETDKA
                     TMEMPCFFEGTLHKIIVGAGEKAPLGAPLAVVLEEGEEAPANLDEIIAKAKAAVAAPA
                     AKAESKPASGASGGGTARKAS"
BASE COUNT         4228 a         6890 c         7007 g         4312 t
ORIGIN      
        1 gcgcgcgcgg ccgccgcgcc cgccgagacg gtggaggaca tcctcggcag cctcagcctg
       61 cgcgcgttgc tggccgcctg tgtgctttgc gcggcctcgg cggcgtatgc ctgcgcggac
      121 atgctggaca cgcgcctgga gcgcccggcc ttcgagcaaa ccctggcgga ggagctttcc
      181 tggccatgat cttccgccgt ctcagactcc gcgtgctgct gctgctggcg ctgtttggcg
      241 cggtgagcgc gggcgtgggt tttttcctcg gcatcgccgc cgccaaaggg gcgcagaaga
      301 aaaaggatga tcccgccgtg tggcggcagg cggcgctccg gcggctggag ggcctgcgcc
      361 cggacgaggc gcagaagccg cgcctcgagg cgcgcgtgga ccaggcagtg aaggatctgg
      421 ccgacctgcg cgtggagggc atccgccgcg tttgggaggt ggtggatgga gcggtgcatg
      481 acattgaggc ggagctgacc ccggaacagc gggaggcctt tgaaaaaatg aagccgcgcc
      541 cgccgaagga ggcgcggtag gcgggcgcgc ttgcctccag cgcgggcggc ggcaagttgc
      601 gctgcgatga agaagcccaa accccgcgtc tgcgccgaga tcgcgcccgg tctcgtgctt
      661 gatccgcgcc gcgcgctggt gcatcgcgcc cagggctgga tggctgtggc ggatgtgcat
      721 tacggctacg agcgcaaccg ccgccggcag ggcgcgctgc tgccggactg gggcatggcc
      781 gcctgcgcgg agactttgcg cgcgctgctg gatgagcacc agccgcgccg gctcatcctg
      841 gcgggggaca tcatggatgg cgcgggttcg gcggaggaga cggcggattt tttggagcag
      901 ctccgcccgc tcgcggaact cgtctgcctc tacggcaatc atgaccgggc gggcttgcgc
      961 aagcgcttga acctggcgcg cgcgcatcgc gaggatgggt ttatttttca tcatggccac
     1021 gagtccgtcg aggctgaggc cggactcatc cacatcaccg gccatgagca tccggccgtg
     1081 aggctgggcg acggcgcggg cctgcgcctc aagctgcccg cgctggtgcg cgagcgcctg
     1141 cccgatggcg gcgagcgctg gattttgccc gccttctccc cctgggccgc gggcggcgaa
     1201 taccactcgt cctgcgggcg catcgccacc tgggtctgcg cgccgggcag ggtgtggcgg
     1261 ctgtgacccg ctgcgggcct tgggccgatc ccgtgcttgc gcgcgcggtc tggcggtgct
     1321 ttttcacgcg ccaggcacaa ctccaaacac acacattcat gccagtctcc gcagttcttc
     1381 ttttctccgg ccagggcgcc caaaaagtgg gcatgggcca ggatctcgcc gccgcattcc
     1441 ccggagcgcg cgccttgttc gagcaggcgg acgcggctct cggcttcccg ctttcccggg
     1501 tgatgttcga ggggccgatg gaggagctga cgaagacaag ccgctgccag cccgcgcttt
     1561 acacgcacgg cctggcgctg ctgggcgcgc tacgcgagcg cgcgcccggc ctggaggtga
     1621 cggcggcggc cgggctttcc ctgggggagt tcaccgcgca cgcggcggcg ggcacgtttg
     1681 actttgccac cggcctgaac ctggtcttcc agcgcggcag ctacatggag caggcctgcg
     1741 aggccgcgcg cggcgccatg gtggcgatga tcggcggcga ggaggaggcg gtgcgcgccc
     1801 tggctgccga gtgtgatgtg gatgtggcga acctgaacgc gccgggccag atcgtgctca
     1861 gcggctgtgt ggagggaatc gaaaaggcag ccgcgcttgc caaagagaag ggcatcaagc
     1921 gcgccatccc gctgccggtg gccggcgcgt atcacagccg cctgatgcgc tccgcgcagg
     1981 aaaaactcgc cgccgctctg gagtcggccg ccatcacacc gccgggcgtg cctgtggtga
     2041 gcaatttcga ggcgcgcccg gtgacagacg cggcggacat ccgcgccaca ctcacggcgc
     2101 aggtgacggg cagcgtgcgc tgggtcgagt ccatgcgcca tctcatcgcc gccgggcacc
     2161 gccttttcat cgagcttggg ccggacacgg ccctggccgg catgctgggc aaaattgacc
     2221 gcgaggcgaa agtcatctcc gtcaaagaca ccgccactct ggatgccgcc gtggctgaat
     2281 tgggcagttg aagcagggtt gccatggccg gccgacttga accccgattt cctccggcgc
     2341 ttcaaaactc aagtgatggt gaagacgcgg acctggatgc cgtttttgcc gctcttttga
     2401 atgctgcgcg gctcgggttg cgcgaagccg ttcaaatcaa cggtgcgggc gcgtatttcg
     2461 tatttgccgg gctttaaatc gcgcagcgtg gtgctgaagc cgaccatgct gtagcgcagc
     2521 ggccatgaga gaggcttgcc gctcgttttg tcgaagccga ggatgtcctg cgggcgtgtg
     2581 ccggcaggca gcgcgctttt ccaatccggc tgcgcatcga gctttgcggg ctgccagacg
     2641 gctttttgcc attcggggtc gttatcggcc aaaacggggc ctgcgggccg cagccacgtt
     2701 tcgacatgcg agaggccgga aaggccgctg atggcccaac ccccgatgtg ggccgttttg
     2761 ccgctcacga cgacgtcatc gagatacgcg gccgttttga ggtgggactc ggggtcgttg
     2821 tttttctccg cgtaggtgtc gttcgcctga tagtcgttgg tgagccggat gtgctggagc
     2881 catttgatgg atttgaagcc gtgcgcccaa ggcacgacca tgcgcacggg gccgccgcgt
     2941 tcgagattga gcggctcgcc attgagcttg taggcgatga agaccgtcag ctcgcccggc
     3001 ggtgtctcca tgacctgcgt gtagctgagc gaggactgga agcgctgctt cgggtcgtca
     3061 ttgtgaaagc cccagaagta aacgcggcgc acgtttgaca tcgcgccgca gagccgcagc
     3121 acctcgcgca gcggaacgcc ctcccacaga ccctgaccga gcggtgtggc gatgttcagg
     3181 cactgcatgg ccttcagaaa cagcacgcga tgctttttgc cgagatcgag gatctgcgcg
     3241 tagtcgagcg tgacggcctt tccgatctgc gccttctgcg tgacgacctc gttcgtcgtt
     3301 tcatcggcgc tgatttcgag cttccacgac tcgggcgtga gtttcgcctt caccaaagcc
     3361 tcgccggtga gcgaatgggg cttcggcgtg cctcgcgaga catcctcaaa atcatccgcg
     3421 ggcgtgagaa acgagcgcgg tttgatctga gcgggttcct ccgcgtgcat cgaggtctgc
     3481 atcgctgcga gcgatccaat gccgagtttc acggcctgac ggcgggagat gggactcatg
     3541 gtcgaggata cggtgctggt gatgtttctt tggcaagact cggagaatgc cttgctcatt
     3601 cccatcactt gtccttcttc ttccccgtct tcttcccttt ctcgctcttc cacagcacga
     3661 tgtcggcgaa gtcgttggtg gagtcggggc cgtcggtgct gcggccacgg gaaatgttgg
     3721 cttggaggag gtcgaggagc tggttcacga tctcgggttt cgattcgaag aggttcgtcg
     3781 tctcgccgat gtcgtccttc atgttgtaga gctgcccctt gggccagttg gcgggcgctt
     3841 tggcctcggt gggcgaggac cagccgccgg aggcgcgggc gagcgcgagc ttccacggtc
     3901 cctggcgata ggcgaagtgg ccgctgatcg agtgatggat gacgccgtga cgcgtggaca
     3961 cgatcttttc accgttgaac gccggcagga tgctcacgct gtcctcgcaa ctgcccgcag
     4021 gcaccggctg gccgaggatc tcggcagcgg tggcgaagaa atcgacgagg cagatgagct
     4081 ggtcgttcgt ggagcctgcg gcgatcttcg ccggccagcg tgcgatgaac gggatgcgat
     4141 gcccgccgtc ccagatgtcc gccttcgagc cgcgcaggtt cgcgctgacc ttgtggccct
     4201 gctcggcgag cttgtcgatc ccggcggcct tcgaacagcc gttgtcgctg gtgaggatga
     4261 agagcgtgtt ctgcttcatg ctattgcgct ccaacgcctc cgacacggcg acgaccacgt
     4321 catcggtctg catcacgaag tcaccgtaat caccgaggcc gctgcggccc tgccactccg
     4381 gcgcgggcac gatgggcgtg tgcggtgaac cgagcggcag gtagagaaag aacggctgct
     4441 cccggccctt gcgcgagtcg atgtactcca ccgccttgcg ccgcaggcgc ggcagcatgt
     4501 gcaccacctc gtcatgctcg atgacctcgt cgttctcgat caccgcctcc atgtcgcgcg
     4561 cgtgatgaaa gccgtggtag tagtcgaagc cgcgggtgat gggcccgtcc gggatcttcg
     4621 cgccgaccgg cggcgtgtcg aactccttcg ctttgtatgg ttggccgctc ttcggatcga
     4681 ggtattcgaa attcagatgc cacttgccga tgatcgccgt gtgatagccc gcgctcttca
     4741 agaaattcgc caccgtgggc cggtccttcg cgatcaaact cggcgcgaaa ccttccacca
     4801 cgccgctttg cagcttcgtg cgccaactgt agcgccccgt catcacgccg taacgcgtcg
     4861 ggctgcacac cgacgaaccc gaatgcgcat ccgtgaagat catcccctgc gacgccagcc
     4921 tgtccgcgcc cggcgtcgcg atcttcccat gctccgggtt caggcaatgc acatcgccgt
     4981 aaccgaggtc atcgcagatg aggaagacga tgttcgggcg ctgctccgca gcgaaagacg
     5041 aactacagag gaaaaggatc agccagaggt gcttcatgtc gccgaaaacg atggcaggct
     5101 tggattctgt ccaccagaat gctgctgaca aacccgacag aggtgttcaa catctgaatt
     5161 gttaaatgac catcacacat atgtctcaag acggtatcga gattcatttc gacgagaagc
     5221 atggccatct gaggctcgcc tgtgagcccg atgcatttgc cgcttatcaa agcgtcgccc
     5281 gcgaacacct caaagacttc ccggatatcc cagtcgataa agtcatcgag atgaacatcg
     5341 tcgacacagc taccttcgtt gcccgtcgcg acgctccaaa acggcgcatt ggaggcttgt
     5401 tctttggcat tatcgtcgtg gtcattatcg ccctggctgt tatcggagct tttgcacttt
     5461 tcaccaaaat taccgcctaa acacatcctg tgaacgcaat cttgaaaacg ctcttgggtg
     5521 ggaagtctcc ggggccacgc aaccgagcaa ctggcggagc ctacaccgtg aacgaacgcg
     5581 acacggtgca gccgctcgca gagtttccgc agtcatccgt tggagctcct actccaatga
     5641 tcctcgcaag tgagcaggct ctattcgtcg cgttttactt ggagcaacgc gacccgaatt
     5701 gggacggcac aacttgtaag atggtcaccc cggacaccaa cgacgagccc gtggcggtgg
     5761 tccgctttag tggtgttacc gcacactttt tcggcccacc taatgatgaa gcatttagcg
     5821 ggcatccttt ggcggagcgt ggcttggaac cttacggggc tttcgagatt ctctcctcgt
     5881 cgtggattcg cgcacttgaa cgaatgaatt ccgttcaccc gcgtcaccat cccgacaact
     5941 tttcgtcttg tcggcatttt gtgctcacct ttcacgacac aattttcgag tgtgtcgcgg
     6001 acagcttcac tattgagcgg ccttctggaa gtttacgctc cgttgcttcc agtcttgccg
     6061 cagctctctg aactcaactg caacggccta ccatgcgttg caacgaacgc tggcgatctc
     6121 tgaggtttgg tcgttggtgc ccatcactct gccttcttct tgttcttctt tttgcccttc
     6181 ttctcgggct tgtccttgcc ggcaccggag ttgaggaact gctggtcgag tgcggcgggg
     6241 cgcgcgtctt tgaactggtc gagggcacct tgcagcttcg ctttcgcggc ggtggcgtcg
     6301 ctggagggac acatcaagct tgccaacgtt tcggtatcac atacgatacc gacatgtttg
     6361 ccgttttgga cacctgtgtt cttgtctctg cgctccggtc gagctcgggg gccagtcacg
     6421 ccgtcctcca agctgtctat gcccggcgca ttcggatcgc cctgtcagtc tccctggcca
     6481 tggagtatga agccgtgtgt tcacgccccg ggctggttcc cgcgctcacc gccggtcaga
     6541 ttcgtacggt tgtggatgtg ttgtgcgccc tggcgcatca gcagaaggtg tttttcacct
     6601 ggaggccgca cctgcccgat gccgacgacg atcatgtctt tgagttggcc gttgcagcgg
     6661 gtgcccctta catcatcaca cagaatttcc gagatttcag aggttcggac tcgctcgggg
     6721 tccagaccat cacaccagcc cgggcactta ccctgatcta gccatgtcca ccatgtccat
     6781 ccgtattcct gactccctcc atcgcggcat caaggagctt gccacgaagg acggttacac
     6841 catgaaccag ttcatcatca ccgccgcagc cgagaagctg gcggcgctga gcaccgttga
     6901 ctacctgggt gaacgtgcca agcgggccga cttcaaggag ttcgagcgca tcatggcgct
     6961 aatcccggca ggtcctcctg atccgggtga tgagcttcct tgatgcgttc tcacagcgaa
     7021 ggctcactcc gccttcttca tgttcttctt tttttgccct tcttctccgg cttgtccttg
     7081 ccggcaccgg agttgaggaa ctgctggtcg agttcggcgg ggcgcgcgtc tttgaactgg
     7141 tcgagggcgc cttgcagctt cgctttcgcg gaggcggcat cgctggcgag ggcggtggcg
     7201 gcgaggtcct gcttctccat ctcgtcggcg ttcaagtcgt agaactggcc ggtgcggtag
     7261 agtttgaagc gctggtcgaa ggcgtattca cgcacggtca tgtccttgct ctgacgcggt
     7321 gagtaccaga tgtagagcca ctcgcgcggc gtgccggttt cgcccttgag ctgcgggaga
     7381 aagctgacgc catcgacgcc ggcgggcgcc tcgacaccgg tggcggcgca gagggtgggc
     7441 aggaagtcgg cgcaactgac gaggtcggcg gagacgcggc cttttttcat gaccgcaggc
     7501 cagctggcga tgcagggcac gtgcgtgccg tgcgcgttgg tggagccctt gccgcctttg
     7561 tagtcgcggc cctggaagcg gctggtgagg ccgctgttcg tgccgttgtc gccgaggaag
     7621 aggatgagcg tgttttcgcg aaggccgagc tcatcgagct ttgacacgat gcggccgacc
     7681 atcttgtcca tgtaggcagt catgtctgcg aagtgcttcg gattctgcag cttctgctcg
     7741 ctgctgatgg tcggatcata gttcgcgctg tccggcgtgg gctggaaggg attgtgcgtg
     7801 aggatcatcg ggtagtagag gaagaagggc ttccctttgt tcttctcgat gaagccgagc
     7861 gcgaagtcgt tcaccagcgt ggggccgtac tcgcccttcg tgaagtcctt ctcgacgccg
     7921 ttgtattcga ggccgggatt cgtgtagcgc ggcgggcggc gcgtctgctg ccagaggcag
     7981 gattcctcaa agccgaagtg ctgcggggag tcgagttcgt ggccgagctg ccacttgccg
     8041 cagatggcgg tggcgtagcc cgcgtttttc atgaggtggc cgaaggtcgt ctcctcgcgc
     8101 cgcagcgtgc cgaagttgag gtagttgcgc acgttgtagc gcccggtcat gagctgcacg
     8161 cgcgtgggcg tgcacagagg ctgcgcgtgg cagttctcaa agcgcacgcc cgatgcggcc
     8221 agcttgtcga ggttcggcgt ctgatacgac tcgcccccgt tcgccgtgac gcactcgtag
     8281 ccgaagtcat cgaccatgat gaggatgacg ttggggcgct gctccgcagc gaatgacgaa
     8341 accagagccg agcaaagcga gacagccagc accgcggagc gtgtgctgct ccgaaggagt
     8401 gagcgaagcg aagcaatggc gaatgaggaa aggagcagga agagaagggc tttcatggca
     8461 tgggagcgct gggttcaacg tgaatgtcat caatttcgat ccctttttcg ccgccattct
     8521 gcatccagag cagcttgcgc ttgccggcgt tgaagttggc gcgggagaga gtcttggacc
     8581 aaagcttgtc gtccacactc attgcgacgg actcaccgtt gaacacgagt ttgacagtgt
     8641 gccaggcgtt cgcgccgaga tcggctcttt cgagcgggag aaattcgttc agccgatacg
     8701 cgccgctcac cttgtcggca ggacgctcct gcttttggcg cagcggatgg tttgggtcga
     8761 gcgaatgcga atcgacctga agttgcacgc cgtcgcgcag cagtttcacg cgcagcatgt
     8821 ggtccacgct gccgaagcca tcgtcaccat cgacgaagaa ccacagcatg ccgccctggc
     8881 cgagatggcg gtagcggaag gagatcgcga gatccttgcc gctcaccggg agatagacca
     8941 tcggcggata cttcccgccg ctgcggccgc gcgtccacag cacgccgtcg cgcacctccg
     9001 tgttcttgtt cgggatcggc gtggtgaagc gtttcgcatc gaagccggcg tcgaaggtct
     9061 ccacgatggg cgcgggtgtg aggtagtcga gtgactcgag gaaggcgtcc acctccgcca
     9121 gcgtcttgta gtagtggagc ttggcccttg cgccggctgc atctttccag cgccccggat
     9181 tgaagaagcc gtgcggctgg ccttcgtagg ctttcaactc gcagcgattg ccggcggcgc
     9241 tcatcacctt ttgaaacaac tgcacggtcg gaaacgatac cgcttcgtca ttcgtgccgt
     9301 ggaagatgat gctcggcgga agtccggcgc gcacgaaatg gtagggcgac acctcctgcg
     9361 gccggccgtc gcagcgttcg cggatgtcgg cgaatttctc ggcggtcagc aaatcaggat
     9421 gcccgtccac cggtgccagg acgacggcgg gattgaacag cagcatcgcg ttcggcacag
     9481 agctgacgcc cttgttgtta tcgtcttcaa agcctggcag cagggccgtg gcggcggcga
     9541 gatgaccgcc ggcggaccca ccgcctgcga cgatgcgatc cggatcgaca ccgagtcgct
     9601 ccgcattcgc acgcacccag cgaatcgccg ccttcgcatc gcgcacgcag tcctgcggga
     9661 agacgttgtg acggcttttc acgcgataat cgaccgagat cgccaccatg ccgcgtttgg
     9721 cgagatgcag gcattgcggc agaaactgcc ccggcgtgcc acccttccat ccgccgccga
     9781 aaaagaacac cgccgcaggc cggtgatccg tcttcgcatg atcctccggc tcgaagatgt
     9841 gcgcgttcag tttcacgtcg ccgattgtgc gatagacctc aactcgcgag ccggtcatct
     9901 ccggcggata tttccaactg atcgtcggct caggcagttc gtctgcggca cggaggctcg
     9961 tgaagaagag aagcgggatc agaaaaactc gggagcgtat ggcctggggg gtcattttgc
    10021 tttcagcgtg gcaatgaaga aggcagttgg cgtcgtgtgc ttcacgtctg ttgcaccggg
    10081 atagacgagt tcgcagggca ccttggtggc gacgcaatgc tcctgcagtt tgacgccgaa
    10141 gttcgaagtg tgcgtcggat ccttctgatc ctggccgagc gcgggcggag cggagtagat
    10201 gagataaacc gggggatcgg cggcggtcac aagcgcatag ggcgaatact ccgcgatcca
    10261 gggcagaatc ttctcgcgtc cggcgaggaa ttccgcaaac gagccgatgc caaaggcgtg
    10321 gccaccgtag cggctgttcg gcgtccactc cttcatctgc tgcgggtcga gcgtggtctg
    10381 cgcacccgtg acggcggcgc actgaagacg cgaagactcg cgggcgacag ggtcactgct
    10441 cgcggggtca gcgaggtcgt catgaaacgc gagccacaga ctcgaacacg cgcccgcaga
    10501 accgccagcc gcgccgacgc gcgtcttgtc gatgttccac tcggccgcct tgctgcgcac
    10561 gaactgcagc gcgcgtgcgg catcgtgcag cggcgctttg accggcggcg tgaccttgtc
    10621 ctccgcggcg tgcttcatga ggcggtagtt gatggccacg accgaaatgc cctgcgcgag
    10681 gagatcctcc acgctgacga acttgttcac gcgctctttc tcgccgctct gccagccgcc
    10741 gccgtgaatg acgaacacca gcggtgtcgg agtggcggac tcggccttcc agaagtcgag
    10801 gatgtgtcgc tcatgcgtgc cgtagggcac attggccatg gtcggcttca cgaccgccgg
    10861 gttggccggg gccgctttct tcttggcggg cgccttagct ttgggattgt cggcggcgga
    10921 aagagccagc gccgggaggc agagaagcag agtaatgaga gcgggtttca tgcagaggtg
    10981 aggttggggc cgtgcgaccg ggacgaaagg aacggagcaa gggggccgac catgcattct
    11041 ttgccgccga aaactcagcc ggcgacgaat gcctggtcgg cacacgatat gctttctctt
    11101 caatccgaag gtgaaacaga acgcctccga acggcgtttt aatggccccc aaacacccct
    11161 catgaatcgt cgtcatttcc ttctcagttc tcttggtgcc tcgctggcgt tgccgagcat
    11221 gtcttctctc atggcgaagt ccgtcggcgg ttcagtgcag gccgccaaag gcgcgggcat
    11281 gggcgcgaag cgtttcgtgg cgatcgggaa cctgctcggc taccagacga agagcctgtt
    11341 tccgacgacg caggggcgga attacgagaa aacgacacta ctggagccgg tgtgggatgt
    11401 tcgcgacaag atgacggtgc tgcgcggtct cgatcacggc gtgaagggcg ggcacttcgc
    11461 ggtgcattcg ttcctttccg gggtgctgca ttcggaggcg cagaatcgcc cggatggcaa
    11521 tgtgaccatc gaccagtatc tcgcggacgc ggtgggcttt gaaacgcggt ttccgtcgct
    11581 caccgtcggt tccgagggcg gcatccatgg cggctgccag atcgcgtgga cgaagtcggg
    11641 cgtgcgggtg ccgccggtga caaatccggc cgagttgttt gaaaaacttt tcatcagcga
    11701 ctcgccggag cgtcaggcgc gtcgtgcgca ggagaacaag gtgcaggcct ccatcctcga
    11761 ctccgtttta gacgaggcga accgcctttc caagaaggtg aatcaggagg acaaggccaa
    11821 actcgacgag tatctgacct ccgtgcgtga tgtggagaag cgtctcgaac tgcgtcagcg
    11881 ctggacgaac cagccgaagc cgaaagcgcc cttcgacaag cctgccaatc gcaacgccgt
    11941 ggaggacatc cctctgctgt atgagctcat cgcgctcgct ttgcagacgg acagcacgcg
    12001 catcgccacg ctggagatcg gcggcgactt catgccgcag catctcggca tcaagaagga
    12061 ctggcacggc ctctcgcatc acggcaacga ccccgaggcc atcgagagcc tcatcacgct
    12121 ggagaagtat cagatcgagc acttcggcaa gttcgtggca cgtctcgcga agatgaacga
    12181 cggcgagcgc acgctgctcg actcgacgac cgtgatgttc ggcagcggca tgggcgacgg
    12241 caattcgcac aagaactccg acctgcccat cctcctcgcc ggtggcggct acaagcacgg
    12301 cgaattccgc gaagtccccc gcgaaggcat caacaaggtg ccgctgtgca atctcttcgt
    12361 cgatatcgcc cagaagatgg gcgtcgaaac cgattcgttt ggcagcagca cgggacgctt
    12421 tgcttgatca gaggtttgaa ccgctgatta gacgctaata tgacgctgat aatgaattca
    12481 gatcattagc gtcatgttag cgtctcatta gcggttcaaa accaccgcaa cacgctcacc
    12541 gctttcgatt ttgaactcaa agaacctcat tcttacctca gcggttttag ccgccacatt
    12601 cgtcagcgca gctgacgcac cgccgaagct cgtgcagcag ttcctgggca aatactgcct
    12661 cgaatgccac gacgcggacg tgcagaaggg cgaccgctcg tttgagaagt tcgagctgcc
    12721 gttgaagtcg gtgcccgatc tcatcgaagc gcgtgacatc atcgaccagc tcacgctgaa
    12781 ggagatgccg ccgaagaagg ccgaccagcc gaaggacgac gagcgtatcg cgatgatccg
    12841 tgcgctgcgt gacggcaccg tggtcgcgta tgggaaattc cagagcaccg gcagccgcac
    12901 ggtgatgcgc cgcctttcca gccgcgagta tgaaaacacg ctcgcggtgc tgtttggtcg
    12961 tcgcgtggac acgctgggcc tcacggccgg ttttccgaag gagaagacca ccgagcacat
    13021 ggacacgatc ggcaagtcgc tcgtgacctc cggcttcctc gtcgatcagt atttccaatc
    13081 cgcgaaccgc ctcgtcgaga cgcgtctcgg caaaccggcg acgccgccga aggattggaa
    13141 gttcaacggc cactttgtgc aatacgagga actcagcggc tcgcacaaaa gcgccttcaa
    13201 ctaccgctac ctgaacattt acgagcagcc aaacagcgac acgcgtcagg gcggctacgg
    13261 ccacatcgag gacttcaagc aaggcgtgcc cgtctccggt ctctacgatc tcgaggtcga
    13321 ggccacggcg atgcatcgcg acacgcatta cgatcccgcc atcttcggca tcgacttctc
    13381 ggagcccttc atcctcggcg tcgtgcctgg cgatgtcacc gcgggccaca ttcattatcc
    13441 gcagccgatt gagcccgtgc tcgccaccgc tgtcgtgccg gacaatgagc ccaagcggct
    13501 gaaattccgc gtgtggctcg aagcagggca aacaccgcgc ttcatcttcc ccaacggccc
    13561 gtatgaatcc cgcgccgccg tcgtcacgat caacaagcgc tacaaagacg agttcaaagg
    13621 cgatgtcggc tcatccggag tcagtcgtgc gcacatttta aaggagggca agctcccgca
    13681 catccgcatc agcgagatcg ccattcatgg tcccgtgccg gaagcacctg gagccgagga
    13741 agtggccgtt tttggcaaag aaggcttcca ggaagccaaa gcgctcgatc aactgaatgc
    13801 cttcgccgag aaggcctatc gccgaccaat caccgaggag gacaaaaagc cgatccgtgc
    13861 gctttatgag aagcgaatcg ccgaaaaagt cgcgccgcgt caggccgcgc tcgatgcgct
    13921 caaattgatc ctttgctcgc cgagcttcct ttacctcagc gagatcacag acgagtcgga
    13981 aaagcatctc aaaccctacg atctcgccac acgcctctcg tatgcgcttt ggtccgcgcc
    14041 gcctgatgac gcgcttctgg ccgctgcgaa gtccggcaag ctcacggagg acgccgagct
    14101 caaaaagcac atccagcgca tgatcgccga tgaccgcatc aacggcttcg tgaacggctt
    14161 cctcgatagc tggctgaatc tgcgcgatct cggctcgcag ccgccgccgc gcgaagtcgt
    14221 gcgttcctac tacgccgaga atctgcccac ctcgatgaag atggaggcgc ggctgttttt
    14281 ccgcgatctg ctcaagaaca acggctccgt cacgcagttc atcgattgcg agcacacttt
    14341 cgtggacaag aaactcgcga aactctacga actgccggag aaggacaaac tgcgcctcgc
    14401 cgatggcttc caaaaagtca gcctcaaggg caacaaccat cgcggcggcc tgctcggcat
    14461 ggccgcggtg ctcaccgtga gtgcgaacgg cgtcgagacc tcacccgtga ctcgcggcgt
    14521 gtgggtcacg gagaattttc ttggtgtccc gccgccaccg ccgccggatg tcgtgcccgc
    14581 gattgatccc gacgtcagcg gtgccaccac cattcgcgac cgactcgcca aacaccgcgc
    14641 cgatgccgcc tgcgccgagt gccatcgcaa gatcgacccg ctcggcttca gcctcgaagc
    14701 gtatgatccc gtcggccgct ggcgcaaaaa atatcccgcc gccaacaaga aggccaaacc
    14761 cgccgaaatc gacaccaccg gcgaattccc ctccggcgaa acgtatgccg acttcaccgg
    14821 cttcaaacgc gtcatccgcg acacccgctc tgacctcttc agccgccacc tcatccgcca
    14881 gctcctcacc tacaacaccg gccgcaccat ggaactcgcc gacgacctcc ccctcgacca
    14941 gctccacgaa aaagtgaagc aacaaggcct cggcctcaac accatcatgg tcgagtgcct
    15001 gatgagtgag gtttttcggt cgaggtgagt gttatgacat gaaggaggcc gcatacgact
    15061 atcttaaact ccttgaggag catgaccata cgcgacggtg tgaataccct ccatcctttg
    15121 acctcaaagc ggctgaggaa cgctttaccc acctggcgga ggagcttatt gctgcttatc
    15181 agggatcaac atttgaaact ggggcggcaa tacaagacgc gagttttcat gggcaaatct
    15241 tccttgcatt cgaaggtctg accatcctca tccgagccag caatttcggc agattcatca
    15301 ctttcttcga cgaagaagag cacatgcttc tgccagacga gactcgttgt tggttgctcg
    15361 aactcttcat caagcatggt tatcggttta ttccgcccga agtgctttcg atgccttaca
    15421 ctggagagtg caaaggtgtc tccggctttc gagactgggg ataccgctac tttgaatgga
    15481 tttgaatttc ctgccgcgaa aactgcacag gattcaccgg cttcggaggt cttgtggcac
    15541 tgatcgcctt cgttgccgca accatttctt cgagcatgcg cagcttctcg cccaccggca
    15601 gtgcggcgag ctgacgacga cgttccacct tccgggcggt gagctggcgc atgaggtcgg
    15661 tcatggttcg aggtaggttt gctggaactt ctgccacttc tcccaaaggt cgtgacgccg
    15721 aaggataggc tcaaacagct ccgcatcaag caccccggcc tccacaaagc ggatcacacg
    15781 gtcatgatcc ttcggcctgc ctacttgcag acaaatggcc gcgaggtgtt ctgcggtgaa
    15841 aactcgcgtc gagacaccat tcacatcccg cgccacggcc tgagccagag cttcctccac
    15901 caacggcgtc ccaggcggca caaactcgac agcccagcct tcgatgatga tgaactgccc
    15961 ctgcggcgaa tagcctctcg cgagcaggta ttcgtaaatc ggccccaaag tgagcaacga
    16021 gccgccaggg gccgtgggca gcatgacgaa aacatcaagg tctttagtcg tgatcggctc
    16081 cagccagaag atggagccca cggcaccacc aatggcatag cgtccgatga cgccatcgga
    16141 ctccatctgg ttgagtacgg caaaaatttt gtccacggtc ttttctaccg cactttcagc
    16201 atctgtggaa tcacgattta attgtacttc actccagcaa ccactgccaa gccgttacca
    16261 ctcgtgtctt cgcaggcagc ggtcgttttg ggaaaccgtt ttgatgctgg gtgatgagcg
    16321 tgcaggtggc tttggggaag acagccgcag catcggccag agcgcggagt tcgcgttccg
    16381 cagtttcggg atcgctggcg tcggcgcaga cctggatgag ttcgtggcgg ccattggcat
    16441 ggcgggcaag gaagtcgatc tcgtagcccg caggcgtgcg gacccatgtg gcggtggagt
    16501 ggcggcgttc gagttcgagc aggacgagcg tctccagggc atgaccgggg ttagcacggc
    16561 ccgtgcggtc gtagagggcg atgagcgcgg tgtcgatggg gtaggctttg cgcgggttca
    16621 ccatgcgctg gcgctctgag ctggcctcca tccaaacggt gcgcacgagg aaggcgtcct
    16681 ccagatgggc gagcaagtcg tgcagcgtgt tttttgccac gggcatgccc tgcgtggtga
    16741 ggaagttgtg tattttctgc acgctgaacg ggctgccggc attcgcgagc agatggcgga
    16801 cgaggaaacg cagcgcggcc acgttcgcga cttgatggcg ctccaccaca tcacgcagca
    16861 tggcgacatc gacgtagctt tgcagcaggc gtgagcgagc gtccaaaggc aggccgagcg
    16921 actctggaaa accaccaccg ctgaggtaat ccgccagcgc cttctcaatg cgcgagcgtt
    16981 tggctggcgt gagcatcgtc acatcctccg gaaggggcac tttttgatgc gcgagcaatt
    17041 cgcggaagct gaaggggtgg atgatgacct ccaacgcccg tccgcgcatg gcggtggcga
    17101 cttcctggct gagcattttg gccgaggagc cgctgaggaa gacctcgacc ttctccgaat
    17161 cgaggatgcg ccgcacaaac gactcccagc ctttgacgag ctggatttca tccaggcaaa
    17221 gcatgacctt ctcgcgcccg cggacgcccg gatgcgtgcg gaaatactcg tcgagcacaa
    17281 aggtcagcgc atcggcctcc aggccgggca agcgctcgtc ctcgaagttc aggtacacca
    17341 gcctttctcg tggaacgcct tgattcagac gctcctgcat ctgctggaaa aggaagcacg
    17401 tcttgcctgc ccggcgcatg ccgatcaccg cctgcgcctt gcccggcagc aaggtcatgg
    17461 gaacctgccg gtgtgtgagc accggcagct cctggctcgt gccaagcgcg agcttttcct
    17521 ggagggcggc agtctggaag gcggtcattt gtctttattt gagagataat tttgcagcaa
    17581 cttgtctttt taaaagagat aattgcttgg gtgcctgtca ggcggcactc gtttacgatc
    17641 ccgtcatgat tcgcttcctg ctcttgtctg caatcatctg catcgccacg cttcgcgctg
    17701 gggaggcggg ttttgtttcg atcttcaatg gcaaggatct gagtggatgg acgactcgtg
    17761 aagccatcaa gggtgactgg aaagtcgtcg atggtgctat cgactgcgat cctcagggcg
    17821 aggggaaggg cgatcgcaat ctgtggacca tcaaggaata cggcgacttc gaactgttca
    17881 tcgactggcg gatcaaggag acgccgttca cgaatccgaa ggagcgtgtc attttaccgg
    17941 acggcagcca tcagaaggac ccgtcgggtg aactggcgac catcgccgtg ccgaacacgg
    18001 attccggtgt gtttctgcgc ggccagcaca agtcgcaggt gaacatctgg tgctggcccg
    18061 tcggctcagg cgaggtctgg ggttatcgca ccgatgccag gttctccgcc gaagttaacg
    18121 cgggcgcgac gccgaagaag aaggcggacc ggccggtcgg cgaatggaac acgtttcaca
    18181 tcgtgatgaa aggcgaccgg ctcacggtca cgctcaacgg tgtggagatc atcacgcagg
    18241 cgcagttgcc cggcattcct gaaaaaggac cgctggcgct gcaactccac cccgaacgca
    18301 aagacggcga gtggggcgca tcgctggtgc agttccgcaa catccggatc aaggagctga
    18361 agtagcttcg aacggcattc gaagaatcgt tgcagaaccc cgaagtctgg cgacttcgcc
    18421 tacatcgcta ggcgcgttcg ccacaacgcg atgcgggctt ccctcacccg ttcttgccag
    18481 tagattccga aacgcgacgg aaagcctttg caacggctta tgccggcacc ctgatcggcg
    18541 tccgatgcaa acatgatgct gttgatcttg tgctcgcgga aagagctgcc gatattcgct
    18601 agcgcaggaa taccgacaag atccggcagg ggtgctctta cttcataacc agcagtgtcg
    18661 tgatgacgac ggcagtgggc acggcagctc cgagagcgag aaagagcgcg gacacgccgc
    18721 cctggaagaa gcgcgagaga atggacaggc cggcagggtt gggcgcgttg gcgatgacgg
    18781 tcaatccgcc cccggccacc gcgccggcca cgacggcgta tttggccatg tctgtgagac
    18841 ccggagcctg cgaggccagg taagtgatgg cggcgttgtc gttgaaggca gtcaggaagg
    18901 ctgatccgag cagcagcacc caatccggca cgccgctggt gaggatgggc tcgatccacc
    18961 acgactggca gcgcccgtgg atgaccaggg caccgaggaa gaagccaacc aggatgggcg
    19021 agcgcaggcc gatgaggttc tggtgatggc gggtggcctc gacgaaggcg atgaagaaca
    19081 ggaagccgcc catgaacatg gccgggtaat ggttggtgag caccgtccag gccaggaagg
    19141 ccgtgtggac caggatcacg cggcgcggca ccgggtcctc gcggtcggcc cagcgcagga
    19201 gccggctttc cccctcctgc ggatgcgcgc ggtggtccag ctcggccagt tcattgcgaa
    19261 accagaaata aaacaaggca ttgccgatga tgatggagat ggcgcacttc cagccgatgg
    19321 tggagaagac aaacgcggtg tcccacttcc acgggctggc caccatgagg attggcggcg
    19381 cggcgaagtg ggtcagcgtg ccgccgatgg agacattgac gaaaagcagc ccgagcgtgc
    19441 cgtaggcgag cgatttgccc gggcgcagcg ggtagatttt gcgcgccagg atcatcgcgg
    19501 agatggtcat cgcggcgggt tcggtgatga aggagccgag cagcgggccg agtgtcagaa
    19561 ccgccagcca ccaggctccc actttgccgc cgccgagcga ggccacggca gccacgcatt
    19621 tttcggcgaa ctggatcacc ggccttgagg cggcgatggc catgatcacc accacgaaga
    19681 gcggctcggc gtagttcaca tcatgcccga gatagagctg cacatcgtgc caggagtgga
    19741 aataaacgcc ggccgccatc agcgccagca cccagatgcc gaagaccacc tccacctcgc
    19801 cgaggaagtg caggatggtg gcgccaaagg acacgtcgtc ctccgcgtcc tcatgaggct
    19861 tgtccttcgc ggtgcggcgc tcgcgctcaa ttttctcgtg gtggacatgc tcgatgtgat
    19921 gcgcccattt gcggaagaag ccggaggcga aagtgtgcag caccgccagg ccgaagatga
    19981 tcgtcgccgc cacgttgaag ggctcctgcg ccgcgcggga catgattttc acccgcgcgc
    20041 tctcctggcc ccggtcgccg taggattcca gcggcctggg gaaatgggcc agttgggcgt
    20101 gcccctcgaa aggcggcttg tcctccagcg aggcggcggc gagggggcag gcgagaagaa
    20161 cgagagaggc gaggagcagg cgcggggtca ttttcatcaa catgcgggag caagtcatgt
    20221 gccgccatac gatccggcgc ggcgggattc aaccggaaga atggggccgc tttgtgacag
    20281 gcgcgtggaa ggcgccggcg tgcggccagg cgctggcgct gttgttcctg ggccgtttcc
    20341 tgctcttgac tcagccgccg ccaaagccga acgatggcgc tcatttttcg acccttcaaa
    20401 acgcccccaa tgccccgccg aatcacctac cgccaagccc ttcgcgaagc ctttgacgag
    20461 gaactcgccc gcgaccccat ggtcgtcctg ctcggtgagg aagtggccca gtacaatggc
    20521 gcctacaagg tgacagaggg cctctgggaa aaatggggag acaaacgcat cgtggacacc
    20581 cccatcagcg aggcaggctt catcggcatg ggcgtcggcg cctccatgct cggcgtccgc
    20641 cccgtcatgg agctcatgtt ttggagcttc tacaccgtcg cctgggacca gatcatcaac
    20701 aatgccggca tggtgcgcta catgagcggc ggcaagatcc actgccccat cgtcgtccgc
    20761 ggcccggcca acggcggcac cagcgttggc gccacccata gccacacgcc ggagaacatc
    20821 atggccagct tccccggcat gaagtgcgtc gtccccagca ccgcctacga cgtgaagggc
    20881 ctcatgaaag ccgccatccg tgacaacgat cccgtcatgt tcatggagag caccaagctc
    20941 tacggcgagg agtgggaagt accctccaac gacgaactcc ccgaaggcga gctcgtcatc
    21001 cccctcggcg tcgccgacgt gaagcgcgaa ggcgccgaca tctccctcat ctgccacggc
    21061 aaagccgtcc tcacctgcct ggaggccgcc cgcatcctgg aggaggagca tggcatcagc
    21121 gccgaagtcg tggacctgcg cagcatccgt ccgctcgatg aggagaccgt gctcaattcc
    21181 gtggccaaaa cccaccgcgc catctacgtc gaggaaggca agcccttctg cggcgtcggc
    21241 gcccagatcg cctacatgat ccaggagcgc atcttcgacg agctcgacgc ccccgtcctc
    21301 cgcgtctgca gcctcgactc ccccgccatc tacagccccc cgcttgaggc cctccagctc
    21361 cccaccgccg atgtcgtcgt ggaaaaggtc ttgagcattt gttaagctcc gcccatggac
    21421 taccagcagc acatcaccat tgatccccag atccggcgtg gcaagccctg catcaaaggc
    21481 acccgcatcg ccgtggccga tgtgttcgac tacctcgccg gcggctcttc caaggaagag
    21541 ctgctcgaag acttccccga ccttaccgaa gccgacgtca tggcctgcct ggccttcgcc
    21601 gccgacaggg agcggcgcta tgccgtggcg atggctggct aaacagcaat tgattgattt
    21661 tcaactgccc cctgtgccgt gagatgaagc tgcttctcga cgaaaacatc tctcacaaaa
    21721 tcgcaggctt gattcagccc tggttccccg gcagccagca tgtccgtgat gccggattga
    21781 agggaagacc cgaccacgaa atctggagct tcgcagccgc aaatggcttc gccatcctct
    21841 ccaaagatgg cgatttcgag gccatttcat tgctgcgcgg ccagcctccc aagtttgtct
    21901 ggattcgcat cggcaattgc acacgcgaga ccttgctcca gttgattctt gcccatcatg
    21961 aagacctgct gtcttttgat gccgatccgg ttgagtccat gcttgtcatt tcttgacgcc
    22021 ttcccttttc acgcttccta aacctccacg ccgtcgtttt ctccgccatg ccaaaaatca
    22081 tcgaaatgcc caagctcagc gacaccatga ccgagggcac cctcgccaag tggatcgtca
    22141 aagaaggcga caccgtcacc aacggccagg ccgtcgcgga tgtggagacc gacaaggcca
    22201 ccatggaaat gccctgcttc ttcgagggca ccctgcacaa gatcatcgtc ggtgccggag
    22261 aaaaagcccc cctcggcgcg ccgctcgccg ttgtccttga ggaaggcgag gaagcccccg
    22321 ccaacctcga cgaaatcatc gccaaagcca aagccgccgt cgcagcccca gccgccaagg
    22381 ccgaatccaa gcccgccagt ggagcctccg gcggcggcac cgcgcgcaaa gccagcc
//
LOCUS       DAOMYJ010000071        21917 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Prosthecobacter sp. isolate AMR_MDS_5629
            NODE_848_length_21917_cov_22.874028, whole genome shotgun sequence.
ACCESSION   DAOMYJ010000071 DAOMYJ010000000
VERSION     DAOMYJ010000071.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867850
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prosthecobacter sp. (wastewater metagenome)
  ORGANISM  Prosthecobacter sp.
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Verrucomicrobiaceae; Prosthecobacter.
REFERENCE   1  (bases 1 to 21917)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 21917)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.30
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:34:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,742
            CDSs (total)                      :: 4,684
            Genes (coding)                    :: 4,676
            CDSs (with protein)               :: 4,676
            Genes (RNA)                       :: 58
            tRNAs                             :: 55
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21917
                     /organism="Prosthecobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_848_length_21917_cov_22.874028"
                     /isolate="AMR_MDS_5629"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1965333"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..598
                     /locus_tag="PLP58_09495"
     CDS             <1..598
                     /locus_tag="PLP58_09495"
                     /inference="COORDINATES: protein motif:HMM:NF024228.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="malectin domain-containing carbohydrate-binding
                     protein"
                     /protein_id="HRJ08791.1"
                     /translation="WLEHPHAGDGNAPRLGVEGLADSATYFRRHSSQAGAGESQPWIA
                     ASGLTGEGEIVIIPSLMKPLLVKPVKKSDDDEDKPSKDKKSESKPVSEPPKPAPLLRR
                     DFPKVRHTVRLHFADPGALPVGERVFSVAIQGKTVLESFDIAREAKTPGATLVREFPG
                     IEIGGELSIRLTAAPGSKAPPVLCGVEFLADTKVGSRE"
     gene            777..6221
                     /locus_tag="PLP58_09500"
     CDS             777..6221
                     /locus_tag="PLP58_09500"
                     /inference="COORDINATES: protein motif:HMM:NF012200.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choice-of-anchor D domain-containing protein"
                     /protein_id="HRJ08792.1"
                     /translation="MKPSLTTLAAKLRGKQNYLLPLLAAALAIPFLQRDNALDSSEED
                     SQALSPNASESSRDLSASSLQRTTTEEASAARMDEKLSVWEAFQKASHEVQLVDAAAA
                     ELPENKGVRFFAANPRQHMTARFMNDGVRLQSGLGGDWQATVAAAAVGRGGRMTSVER
                     AGAPSAAGETVNYEMSGVTEWYKNKPEGIEHGFTINEAPQVGAGGELRVDVSVSGLSA
                     KNHESGIELRNAGGEPVLGYDKLKVWDATGRELPSRMTAVADTISILVADAGAVYPVT
                     IDPIFTSYEAKLEIGELECGKPGDFLGHSVGVFGDLAAVGAPGDDSLLGTDVGAAYVF
                     ERSDTTWSIAAKLKAVDGANGDRMGGSIAVGDGFVLVGARLADLKNKPDAGAVYAFTK
                     RMGMWMQTSKLTAKDAVSGDWFGESLAVDGDTAVIGAPNFVNNRGAAYIFARAGASWK
                     QVTRLLAAGGVSNDRFGESVDIKGNTALVGATGQGGKGAAYVFGFNGTVWSQLQMLSD
                     TGGATGDFFGDAVGLALDCAVVGAPGRSSSTGAAFLFTKSGMTWSSSPVQTLTAGDGA
                     AGDMFGRSISQYGKLALIGAPGATVSSTEDRGAAYLFGPESTATWVQQSKLTAPSGVA
                     GDWFGGSVALHQTTAVVGAFQADAPGWDGGIVVDLGCAHVFRVFSSSSPKVVIKETSN
                     IEEEDAEDVENGDTLNFGTVLLGLNSSRVYVIRNQGSQPLLNLKAVITGSNANQFSIV
                     KAPPSSLEPGESFEMELVFTPTSTGSKQSTLRITSNDTSAATVDVTLLGVGDDAEEPE
                     VDDPDDQIVAVGSQARFEVRAGGSPTPSLQWRKNNAKINNKTSSVLSFRAALTDAGKY
                     DVEAKSKGGTAISAPAYLAVVDTTETNLCLPSGGKAVMKVIAAGTNLSFTWFKGTTQI
                     IQNSRITVSADGKTLTINDLEPGDNGLYRCAVSGPGGSLFGGIHYLTVVTGAPQMSQF
                     SLPDGHVSCPYNFQVPFNSSSDFKPTKFTATGLPKDLVIDPMTGRITGIPAGPGGTTH
                     SVKITAMNAKGSAVVNTSIHIHPLPCGVAGKFKGIVKRDGGLNGGFGGMIDMDINSSA
                     SISGVLTLGADKHPFTSSLSCPSQSMGAFGSITVERKGLPSLSISFHINLSTGTLTGT
                     ILDSSNTMWTFAIVAGGAFQAGSVDGMNTAARFNCPYGLTKDKFGNIYVADSANHVIR
                     RFSPSGQVLTFAGVAGMSGLANGCESEVRFNSPLGVAADAMGFIYVADTGNRVVRKIT
                     PEGKVSTLAGSGASGVADGTGSSASFVAPTGIFVASDGAIYVTDADAHNIRKITPDGV
                     VTTLAGKANVSGAKNGTGTAAEFYSPKGVTEVSPGILVVADTSNNCLRRIIIKTRAVT
                     TFAGGLGVGGDADGKVVNARFWYPTGVTADAVGNVYVADTGNGTVRRVSRHGVVTTIT
                     DSCDCERPRTAGLAGDGHHENSISQEPYGCCLSCHQYSPFYQPVGIVLSGKNLVVSDC
                     YMHVLQAGFAESACDVDFEAWRNPWGNDPYPQQSMTTMTTPYPCFNPPASSYAGTYNF
                     ILEPCDDYYDNSLTPAVLMGASIPQGNGVGMLTVNSNGTVNCTATLADGTTVTGSSIL
                     GGVYYSSSGYVPLHFMLYKNTGSVQGVHIISYGYPNDNLGHQPQTPSSPSNYVDGCMD
                     WMKISQLPPAGKNYPEGFPLHGLQVIGEQYDSNSYANLVGSFFNSGSNNALLEFFQGG
                     LSPEISKIFTFNANSTATMPDSSVSFSFNKANGKFSGTFKHGSPQMTTSYQGVFLRRI
                     YSGGGFFLHDLGSLILSGQAWFGPYYSFPD"
     gene            6348..6893
                     /gene="ssb"
                     /locus_tag="PLP58_09505"
     CDS             6348..6893
                     /gene="ssb"
                     /locus_tag="PLP58_09505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009958577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HRJ08793.1"
                     /translation="MASLNKVMLIGNLTRDPDVKFTPKGSAVCDIGLAVNRVYTNDNG
                     ERIEEVTFVDVTLWGKTAELAGKWLYKGRPVFIEGRLQLDTWEDKQSGQKRSKLRVVG
                     EQMQFLGSPQGAGDRSGGGDEGGYGSPPPQQRRQAGGGGGYQQRPAAQQRPAPQQRPA
                     AEDDFGEGPITDDVDGDEIPF"
     gene            7031..7261
                     /locus_tag="PLP58_09510"
     CDS             7031..7261
                     /locus_tag="PLP58_09510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ08794.1"
                     /translation="MKTLIPALLLSALTLGTALAKSGPPVNDLCPVDGKAVRIIYRIF
                     SERGNVAFCCTECMETWRKNPGRYPVKPRIEK"
     gene            7313..8611
                     /locus_tag="PLP58_09515"
     CDS             7313..8611
                     /locus_tag="PLP58_09515"
                     /inference="COORDINATES: protein motif:HMM:NF002461.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0006835 - dicarboxylic acid transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dicarboxylate/amino acid:cation symporter"
                     /protein_id="HRJ08795.1"
                     /translation="MHTSPARAPWWRVLYIQVLLAVAAGILLGWLFPDTGKTLKPLGD
                     GFIKLIKMMIAPVIFCTVVHGVASMGDLKKLGRVGGKTLLYFEVVSTLALVIGLVVVN
                     LLKPGAGFNVEVADLGAKAADAAREYAGKAQGLSTVDFLLGIIPGTFVGAFAGGDLLQ
                     VLFIALLAAFGLAGMGERGKSLLHGIDRVSEVFFAIMRIVVKAAPVGAFGAMAFTIGS
                     YGLESLQRLLALMLGFYITAGFFVVVVLGLIARWCGFSIFRFLAYIKEELLLVLGTSS
                     SETALPLMIQKLRGLGCAPATVGLVVPTGYSFNLDGTNIYMAMAAVFLAQATNTPLDL
                     GQQVSLLLVAMITSKGASGVTGAGFITLAATLAVVPGIPIESLALLVGIDRFMSECRA
                     LTNLIGNGVATVAISRWEGEVTPEVINANLRDPVRLPPPA"
     gene            8734..9960
                     /locus_tag="PLP58_09520"
     CDS             8734..9960
                     /locus_tag="PLP58_09520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009959489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HRJ08796.1"
                     /translation="MIFSLSSRILTTVDPKCLAKIAWNFGFKGARSVMLFKKRMEQGQ
                     FFPPFLYISILNSCNLRCQGCWVDVDKPREAISLDELNKIINDAKRHGNAFFGLLGGE
                     PFMHPELLDLLALHPDCYFQIFTNGQLITAKTAEALRKLGNATPLVSVEGNSTVSDER
                     RGNKDVLNRTLRGLRNCLDARLLTGVATSLCQTNIGDLLTREWLEKLVSMGVHYTWYH
                     TYRPVGPQINEQLALTPAQITEVRRFVTSMRARVPIAIVDAYYDHNGKALCPMATGIS
                     HHIGPTGGIEPCPIIQFAKETVRTERDIFDVFTQSEFLKDFRETAARHTRGCIVLERP
                     DLVKELALRHGAKDTTIRQTAMAEVESMKPRTSQWREEEEIPEQHWMYKVAKRFFYHD
                     FGVYEKMDPKAHSPAA"
     gene            10003..10236
                     /locus_tag="PLP58_09525"
     CDS             10003..10236
                     /locus_tag="PLP58_09525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ08797.1"
                     /translation="MSTLLEQIRALPVDEQMALAGDALESSGEGNDSPGLAPSLQVEL
                     ERRIDEHLAHPGRAATLEAARQRNNESVESRAA"
     gene            10643..10975
                     /locus_tag="PLP58_09530"
     CDS             10643..10975
                     /locus_tag="PLP58_09530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009959487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ08798.1"
                     /translation="MPDTPNLLYCRCAYAQVVPAGVKNEVLQRLCDSGASFETVSDLC
                     EMAAHRDPRLQELVAAGRLRIAACYPRAVHGLFHQAGAPLPEDTEILNMRTQGAEEVA
                     AKMLRGEN"
     gene            complement(11044..14997)
                     /gene="smc"
                     /locus_tag="PLP58_09535"
     CDS             complement(11044..14997)
                     /gene="smc"
                     /locus_tag="PLP58_09535"
                     /inference="COORDINATES: protein motif:HMM:NF014516.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein SMC"
                     /protein_id="HRJ08799.1"
                     /translation="MYLKSLTLHGFKSFADKTHFEFHKGVTGIVGPNGCGKSNVVDAI
                     RWVLGETSAKALRGDEMADVIFNGTDKRKPVGMAEVVLTMADCEQALNVDYNEVAICR
                     RVFRDGRSEYRLNGTVCRLKDIHELFSGTGIGRQAYSIMAQGQIDMLLSSKPEDRRTV
                     FEEAAGITKFKGQKKEALRKLDYTEANLLRVADVIAEVKRQMGSLQRQAAKARRYQAL
                     LDDVRMFDTHLAHKHHSEFSAEKAESENHVRMLTGQLDALQNTLQTTETEAAQTREAY
                     HAVESQINQLRQQAQELRSQVQSAEGRIGFNNERNEELQGRIRQNEEQIGQGQQLLEQ
                     QRREMADADEQVLAISANLETRRNAVHEHLSVHNAILPDRGRLDEARRAVRESIRQFE
                     GQIASADARAQSLTNQISADRQRHETLAHDHQAAAQARESSQIEFDEIQHRIHDLEQT
                     RNELEDRSKQCARDILDKRARRDALNEELNELQRAVTQRRSRVEILEQLLQKGEGLAE
                     GTQQVLKGMDNPEIYSIGVRGLLASAIEVEPQFIAAIEAALRDHLQAVLLTDSELASQ
                     ILERLSNHQLGRAALIPQDLATLRAVPDRQLVPGGSIAWAVEKVRARPGAQEITDRLL
                     SNVLIVEDLHTALRMKRQLPDAAIVTLKGELLGADGIIHGGASKEEAASMLRRQSEVG
                     TLKAELENFEYQLLEKEEAVEQLRAQLEEAQREEISLREQSQRAREGFSQLQGQLSVV
                     QRALQQATTRLESIEWDQNQITSRIQSAEAQITWQREQAGLAQEQLEGARMHEGELEL
                     EMESFLRRELESSEKLNELRTALALEESALNSLNRQKAPMAARLHELESSIQRFDQEI
                     CTWRSRIEQSAAESARFAEQIESQRGAIAAIEEHLQGRTEERAAAFERVSLLENQLVQ
                     LRQQISGLSESRSRLEVQLTRVDLRLENLVNQVQERYSFHISAFEPDYHALMLSITEQ
                     KKGRSRVEKRKATLEMETQATVEQTPGQVEESEAQETAEPSIQPEVSDSSDLSNAPGA
                     TCTALEDDEIVDLDEIEIPGEEGQPDWEFVTSIVGDLRQKLESIGPVNLDAIQEFEEL
                     EERHNFLETQHGDLVKSKEELLQVIAKINETTKTMFAETFQLVRNFFQNNFKELFGPQ
                     AKADLMLVDETDPLESGIEIIAKPPGKKLQTISLLSGGERSMTAVALLFSIYQVKPSP
                     FCVLDELDAPLDESNISRFLKMLDNFIENSQFIIVTHNKRTMGRADVIYGVTMQEFGV
                     SKPVGVRMTQEEKKPSKAEQLRLEAEVAAEEAHHPDGIPVEDAAGEDEPSPALAS"
     gene            complement(15177..15818)
                     /locus_tag="PLP58_09540"
     CDS             complement(15177..15818)
                     /locus_tag="PLP58_09540"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF012521.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HRJ08800.1"
                     /translation="MKPHLPNHPTQLYLIRHGEVEERYHKVFGGSRIDMGLSDLGRRQ
                     ARAVAAWLADTSLDGIYASPLLRVQETLAPLAQASGLKPVIIPSLREVDFGDWTGYRW
                     EGVKEHFGVSAFDWLEIIEEAGIPNGESGHELAHRVRPALEKILHDNPHRRVAVFCHG
                     GIIRVILALLLGQPLKHMAHFNIEYGSISVVEIQPEKKHAFEIELLNYCPPLA"
     gene            15934..17379
                     /locus_tag="PLP58_09545"
     CDS             15934..17379
                     /locus_tag="PLP58_09545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007417258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HRJ08801.1"
                     /translation="MTNRRRFLVQSAAASTGVFYIAKTSWAQKSPGDTLNVAVVGFGG
                     RGGSHISGYKKLDKEGVRVAALCDVDSKVLDRGVDAFDKEGKKVARHTDIRRLLENKD
                     IDAVSIATPNHWHALGTIWAVQAGKDVYVEKPVSHCVWEGRQMVKAARKYSRIVQTGT
                     QSRSSRKGIYEAVKWVRAGNLGKINVVRGLCYKRRDSIGKAEGAQPVPETVDYDLWCG
                     PGPLADLTRKRLHYDWHWSWAYGNGDLGNQGIHQMDIARWFLGEMELSPLVWSIGGRL
                     GYEDDGETANTQFIYHGYEKAPLIFEVRGLPQAAGVKDMDKYKGAGVGVIVECEKGYV
                     VVPSYSKATAYDLKDQMILEWSGSEDHFANFINACRSRRVDDLHADILEGHLSSALCH
                     TGNISHRLGAKEKPEAILERVKDYRTGTETFERMKEHLAKNGVDISKDLLTLGPVLGM
                     NPETERFTGNDAADAMLKTNYREPYVVPEIV"
     gene            complement(17384..18535)
                     /locus_tag="PLP58_09550"
     CDS             complement(17384..18535)
                     /locus_tag="PLP58_09550"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="sialidase family protein"
                     /protein_id="HRJ08802.1"
                     /translation="MPPFQPAPPAMNTRASLRLLCLCSAAALSAPAQSALDVLFDPLL
                     PMPNRQPGQPERPGRQQRGPWENDVLVHQLGKDGGAPQVAVFERAGVPTLTRLRDGRL
                     LAAHQHFPKNDPGNFDKIAVHFSSDEGLSWTPARVMQVEGLPEGMRFPFDPTLVTLPD
                     GRVRLYFTGMTGRVFSENRPRIHSAISEDGVNYRYEEGARFEIENRPVIDCAVVLHQG
                     VFHLYAPDNGGRPENGGEERPRQGAGFHATSTDGLNFTRAEDVSLPGRLRWLGNAQSN
                     GATITFFGTSDGGGDPRADGGSPRGGIFMAVSSDGQEWRLIESPGVSGADPGAAATPD
                     GGWIIAVTGPPVEGRSRPSAQPPGQNPAPDDAWLRRWALEVLLDEALKK"
     gene            18704..19909
                     /locus_tag="PLP58_09555"
     CDS             18704..19909
                     /locus_tag="PLP58_09555"
                     /inference="COORDINATES: protein motif:HMM:NF015764.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HRJ08803.1"
                     /translation="MQPAAAASQTGRLPLVSLFFFTAMPLGMWNVPLANVFAAHGRAH
                     LVPYVLATSAVAAFISPLFVGALADQRLSPTTLLRWLCLGTGAGLALICTALARGMND
                     GMILLCAQALSLVSAPLWGVTTSIVLAQLREPKREFGPIRACATFGWMGGCWVVSFVL
                     KADNSLVAGYSAAALWLGVAALTWTLPKTAPADVQAGRNWRQVLGLDALDLLKNRDHR
                     VVFITAALYSIPLAAFYPYTARQLHDLEVESVSAVLSLAQTTEILTMLLLAKVLARAR
                     LKWVFLGGIAICVGRYALNALDTRGWVMAGTTLHGFAFTLYFITAQIYLEERIDPKWR
                     VRAQALLTLLMGGVGNLLGYLGGGWWHGLCVKDGVTDWQRFWLGETALTAAVCVFFAL
                     AYRGRGARA"
     gene            complement(19962..21743)
                     /locus_tag="PLP58_09560"
     CDS             complement(19962..21743)
                     /locus_tag="PLP58_09560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009964287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase subunit alpha"
                     /protein_id="HRJ08804.1"
                     /translation="MSTTPTPTASATKTLRNAVIRLAGNSQDGIQSIGGFLARLAGRT
                     AKDVMTYMTIPSTISGGPSIFQLHLGSGEVLHPGDESDVLVAFYQHSYNDHLHALRDG
                     GICIYDSGEVAEVKSERGIHHIGIPINAATIEAIGGSARDRGKNMFVLGLLTAVFQLD
                     REKLSGIIARQFGKKDESVLRNAMLAFDAGYAYPVGDIGSFHFDEGEHKDEHRLSTDG
                     NTLMTMGLICAGVRYGAAYPITPWSPIMEQLRAELPKYGGIYVQAEDELSAVSMTIGA
                     AFAGHLAVTGSAGPGLSLKMEALSYASMAELPLIVINVQRGGPSTGLPTSVEQSDLLQ
                     AIYGSHGDCPRIVLAPQDVEDCFYIALEAGKLAREYSCPVIILSDQALSSRIEAFTEP
                     DLDKHWVEPVLDLSDRPEDFKPYPLDKITRHAPPGAKMLGGRYPHVTGLEHDEWGHPS
                     GNPKMHQKMTDKRRNKLVELAEKLPLPEIYGEQEGDVLLVGWGSSYGPIKESVNRLRD
                     EGHKVGAINLRHLHPMPARLDGIFAKFKNVVVVELNDVGVYGYGQLAMLLRAKLADPK
                     IQSLCKTDGLNFRIREIVTGVEKVLAS"
BASE COUNT         3846 a         6758 c         7008 g         4305 t
ORIGIN      
        1 ctggctggag cacccgcacg ccggcgacgg caacgcgccg cgcctcggtg tggagggtct
       61 cgccgacagc gccacttatt tccgccgcca cagctcgcag gccggcgccg gggagagcca
      121 gccctggatc gctgcctccg gtctcaccgg agaaggtgaa atcgtcatca tcccatcttt
      181 gatgaagccg ctgctggtca agccggtgaa aaagtccgac gacgatgaag acaagccctc
      241 caaggacaaa aagagcgaaa gcaaacccgt gtcggaacct ccgaaacccg cgcctctgct
      301 gcggcgtgat tttccgaaag tgcgccacac cgtgcgcctg cacttcgccg accccggcgc
      361 gctgccagtg ggcgagcgcg tgttcagcgt cgccatccag ggcaaaaccg tgctggagag
      421 cttcgacatc gcccgcgagg caaagacacc cggcgccacg ctggtgcgcg agttccccgg
      481 tatcgagatc ggcggcgagt tgagcatccg cctcaccgcc gcccctggct cgaaggcgcc
      541 gccggtgctg tgcggcgtgg agttcctcgc cgacacgaag gtgggcagcc gggagtgacg
      601 cccggcttcg aaaatacttt gcatgagttg aacttcggcc ttgcgcattc agggccaaat
      661 ttggcgcaga gatttgagaa aaaagtcttg ctcctttgag gtgaaatgcc gcaatgtctc
      721 caccagcact tcggtaattg ttccaattcc gtaattcaat tcgtcccctc catcccatga
      781 aaccgagcct aaccaccctc gctgccaagt tgcgcggcaa gcaaaattat ctgctgccct
      841 tgctggcggc ggcactcgcg attccgtttt tacagcgaga caacgcgctg gattccagcg
      901 aggaagacag ccaggcattg tcaccaaacg cttcggagtc atcgcgggat ctttcggcat
      961 cttctttgca gaggaccacc acggaggaag cttctgcggc acggatggat gagaagctct
     1021 ccgtgtggga ggctttccaa aaggcgtccc acgaggtgca gcttgtggat gccgccgctg
     1081 cggaactccc ggaaaacaag ggtgtgcgct ttttcgcggc caacccgcgc cagcacatga
     1141 ccgcgcgctt tatgaatgac ggcgtcaggc tgcagtccgg tctcggtgga gactggcagg
     1201 cgactgtcgc cgccgccgcc gtgggacgcg gtggccggat gacatctgtc gagcgcgccg
     1261 gcgcgccttc cgccgctggc gagacggtga attatgaaat gagcggcgtg acggaatggt
     1321 acaaaaacaa gcctgagggc atcgagcacg gctttaccat caacgaggcg ccccaggttg
     1381 gcgccggtgg cgagttgcgg gtggatgtct ctgtgagcgg cttgagcgcg aagaaccatg
     1441 aaagcggcat cgagctgcgg aacgccggag gcgagcctgt gctgggctat gacaaactca
     1501 aagtctggga cgccaccggc agggagctgc cttcccgtat gacggcggtc gcggacacca
     1561 tctccatcct cgtcgcggat gccggcgcgg tctatccggt gacgattgac cccattttca
     1621 cctcctacga agccaagctg gagatcggcg agttggagtg cggcaaaccg ggtgacttcc
     1681 tcggccactc agtgggtgtt ttcggcgatc tggccgctgt cggcgcgccg ggggatgaca
     1741 gcctgctggg gaccgatgtc ggcgcggctt atgtcttcga gcgctcagac accacctgga
     1801 gcatcgcggc caaactgaag gctgtggatg gcgcgaatgg tgaccgcatg ggcggctcca
     1861 tcgccgttgg cgacggcttt gtgctggtgg gagcgcgcct ggcggatttg aaaaacaagc
     1921 ctgacgccgg cgcggtgtat gccttcacca agcgcatggg aatgtggatg cagacctcga
     1981 aactgactgc caaagacgcc gtctccgggg attggtttgg cgagagcttg gcggtggacg
     2041 gcgacacggc ggtcatcggc gcgcccaatt tcgtcaacaa tcgcggcgcg gcttatattt
     2101 ttgcccgcgc gggagcctcc tggaagcagg tcacacgcct gctggccgca ggcggtgtct
     2161 cgaacgaccg ttttggcgag tcggtggaca tcaagggcaa caccgcgctg gtgggcgcga
     2221 cagggcaggg tggcaagggc gcggcttatg tcttcggttt caacggcacg gtctggtccc
     2281 aactgcaaat gctcagcgac accggcggcg ccaccggtga tttctttggc gacgcggtgg
     2341 gcctcgccct ggattgcgcg gtcgtcggcg ctccaggcag aagcagcagc acaggcgcgg
     2401 catttctttt cacgaaaagc ggcatgactt ggtcatcctc gccggtgcaa acgttgaccg
     2461 ccggggatgg cgcggcaggc gacatgtttg gcaggtcaat ttcccaatac ggcaagctgg
     2521 ccttgatcgg cgcgcctggg gcgacggtca gcagcaccga ggatcgcggc gcggcttatc
     2581 ttttcggccc ggagagcact gccacatggg tgcagcagag caaactcacc gctccaagcg
     2641 gagtcgcggg cgactggttt ggaggttcgg tggccctgca ccagaccacg gcggttgtcg
     2701 gagccttcca ggctgacgct ccgggttggg atggcggcat cgtggtggat ctcggctgcg
     2761 cgcacgtctt ccgcgtcttc tcctcatcct cgccgaaggt tgtcatcaaa gaaacaagca
     2821 acattgagga ggaggacgcc gaggatgtcg aaaatggcga cactttgaat ttcggcacag
     2881 tgctgcttgg tttgaattcg agccgggttt atgtgattcg caaccagggc agtcagcccc
     2941 tgctgaactt gaaggcggtc atcaccggca gcaacgccaa ccagttcagc atcgtcaaag
     3001 ccccgccctc ctctctcgag ccgggcgagt ccttcgagat ggagctggtt ttcaccccca
     3061 ccagcaccgg gtcgaagcag agcacgctgc gcatcaccag caatgacacc agtgcagcca
     3121 cggtggatgt gacactgctg ggtgtcggcg atgatgctga agaaccggag gtggatgacc
     3181 ccgacgatca aattgttgca gttggctccc aggcgcgctt cgaagtcaga gctggcggca
     3241 gcccgacacc cagtctgcaa tggaggaaga acaacgccaa gatcaacaac aagacatctt
     3301 ccgtgctgtc cttccgcgcg gctctgactg acgccggcaa gtatgacgtg gaggcaaaaa
     3361 gcaagggagg cactgccata tccgcgccgg cttacctcgc cgtggtggac accacggaga
     3421 caaacctttg cctgccttcc ggcggcaagg cggtcatgaa ggtgatcgcc gccgggacga
     3481 atctgtcctt cacctggttc aagggcacga cgcagattat tcaaaacagc cgcatcaccg
     3541 tgtcggcgga tggcaagacg cttaccatca acgacttgga accgggtgac aacggcctct
     3601 atcgctgcgc ggtctccggc cctggcggct cgttgtttgg aggcattcat tacctgaccg
     3661 tcgtcaccgg cgctccgcag atgtcgcaat tcagccttcc ggacgggcat gtcagttgcc
     3721 cctacaactt ccaggttccc ttcaattcct cctccgactt caaacccacc aagttcaccg
     3781 ccacagggct gcccaaggat ttggtcattg atccgatgac cgggcgcatc accggcatac
     3841 ccgccggtcc cggaggcacc acccactctg tgaaaatcac cgcgatgaac gccaagggca
     3901 gcgccgtggt gaacacctcc atccacatcc acccgctgcc atgcggagtc gcgggcaagt
     3961 tcaaaggcat cgtcaaacgc gatggcggtc tcaatggcgg ctttggcggc atgattgaca
     4021 tggacatcaa cagctcggcc agcatctcag gcgttctgac gctcggcgct gacaagcatc
     4081 ccttcaccag ttccctctcg tgcccctccc agtccatggg cgcctttggc agcatcaccg
     4141 tcgagcgcaa ggggctgcct tcgttgtcca tcagcttcca catcaacctt tccacaggca
     4201 ctctcaccgg gacgatcctg gattcctcca atacgatgtg gacgtttgcg attgtcgcag
     4261 gcggcgcctt tcaggcgggc agtgtggacg gcatgaacac ggcggcgcgc ttcaactgtc
     4321 cctacggcct taccaaggac aagtttggca acatttatgt ggcagactct gccaatcatg
     4381 tgatccgccg gttctcgccc tccggccagg ttctcacctt cgctggcgtt gccggcatgt
     4441 ccggactggc aaacgggtgc gaatccgagg tgcgcttcaa ctctcccctg ggagtggctg
     4501 ccgatgccat gggcttcatt tatgtggctg acaccggcaa ccgtgtcgtg cgcaagatca
     4561 cgcccgaggg caaggtctcc acgctggcag gatcgggtgc ctctggcgtc gccgacggca
     4621 ccggttccag cgcttcgttt gtcgcgccca ccggcatttt cgtggcctct gatggcgcca
     4681 tttacgtcac cgacgccgac gcgcacaata ttcgcaaaat cacccctgac ggcgtggtca
     4741 ccaccctggc gggcaaggcg aacgtgagcg gggccaaaaa cggcacggga acagccgccg
     4801 agttttacag tccgaaggga gtcaccgaag tcagccccgg cattctggtc gtcgccgaca
     4861 ccagcaacaa ctgcctgcgc cggatcatca tcaaaacacg cgctgtgacc accttcgcgg
     4921 gtggtctcgg tgtgggtggt gacgcggatg gcaaggtggt caacgcgcgc ttctggtatc
     4981 ccacaggtgt cactgcggac gccgtcggca atgtctatgt ggccgacacc ggcaacggca
     5041 cggtgcgccg ggtcagcagg catggcgtcg tcaccaccat caccgattcc tgtgattgtg
     5101 aaagaccgcg gacagccggt ctggcgggtg acgggcacca cgaaaacagc atttcccagg
     5161 aaccctatgg ttgctgtctg tcctgccacc agtattcgcc attctaccag cctgtgggca
     5221 ttgtgctgtc tggtaaaaat ctggtcgtgt ccgactgcta tatgcatgtg ctgcaggcgg
     5281 ggttcgcgga gtccgcttgc gacgtggatt tcgaggcttg gagaaacccg tggggcaacg
     5341 acccttatcc gcagcagtcc atgacgacca tgacgacacc gtatccttgc ttcaacccgc
     5401 ccgcgtcgtc ctatgccggc acctacaact tcattctgga gccttgcgac gactactatg
     5461 acaactctct cactcctgct gtcttgatgg gggcgtccat tccccaaggc aacggtgtgg
     5521 ggatgctcac cgtgaacagc aacggcaccg tcaattgcac agccactctg gcggacggga
     5581 cgacagtcac cggcagcagc atcctcggcg gcgtgtatta cagcagctcg ggttatgtgc
     5641 cgctgcactt catgctctac aaaaacaccg gctccgtcca gggcgtccac atcatttcct
     5701 acggttatcc caatgacaac ctgggccacc agccgcagac gccgagctcc cccagcaact
     5761 atgtggacgg ttgcatggac tggatgaaaa tctcccagct tcctccggcc ggcaaaaact
     5821 accccgaagg cttccccctg catggtttgc aggtcattgg cgagcaatat gactccaaca
     5881 gctacgcgaa cctggtcggc agcttcttca acagcggctc caacaacgcc ttgctcgaat
     5941 ttttccaggg aggcctgagc ccggagattt caaaaatctt caccttcaac gccaacagca
     6001 ccgccaccat gcccgacagc tcggtcagct tcagctttaa caaggccaat ggcaaattca
     6061 gcggtacctt caagcatggc agtccgcaaa tgaccaccag ctaccagggc gtcttcctgc
     6121 ggcgcatcta ttccggcgga ggcttcttcc tccatgatct cggctcgctc atcctctccg
     6181 gccaggcatg gtttgggcca tactactcct tccccgattg agcgggtctt catgaagttg
     6241 cggatggcgg agcgggaaac cgctccgcca tttttatttt taccgggtga cctacttgcc
     6301 ggaaaaaagg tttgcccatc cgcccggcct ctggctaaga tgccgtcatg gcctccctca
     6361 acaaagtcat gctcatcggc aatctcaccc gcgatccgga cgtgaagttc acgccgaaag
     6421 gcagcgcagt ctgcgacatt ggccttgccg tcaatcgtgt ctatacaaat gacaatggcg
     6481 agcgcatcga ggaggtgact tttgtggatg tgacactttg gggcaagact gcggagctcg
     6541 ccggcaaatg gctctataaa ggccgtcccg tgttcattga gggccgcctg caactcgaca
     6601 catgggagga caagcaatca ggccagaaac gcagcaagct gcgagttgtc ggcgagcaga
     6661 tgcagttcct cggcagcccg cagggcgccg gtgaccgctc cggcggcgga gacgagggcg
     6721 gctacggttc gccacccccg cagcagcgcc gccaggctgg agggggaggc ggctaccagc
     6781 agcgtcccgc cgcgcagcag agacccgcgc cccagcagcg cccggcagcg gaggatgact
     6841 ttggagaggg accgatcact gatgacgtgg atggtgacga gatacccttt tgaagccggg
     6901 cgccttgtgg aacgagccac gattgcgcaa caatgcgcgt gaattggcat ggcactatga
     6961 tgagggcggg tgtcctcgtt gggaagtaaa gcgcgcatgt tgccgcgcgg cagaccacct
     7021 catcccagcc atgaaaacgc tcatccccgc actcctgctc agcgccctca ccctcggcac
     7081 cgctctcgcc aaaagcggcc cgcccgtcaa tgacctctgc cccgtggatg gcaaggccgt
     7141 ccgcatcatc taccgcatct tcagcgagag ggggaacgtg gccttctgct gcacggaatg
     7201 catggagacc tggcggaaaa accccggacg ctatccggtg aaacccagga tcgaaaaata
     7261 atccgtgctt gcctgttggc gggggaacgg cgtttcatgg ccgtctcccg ccatgcacac
     7321 ttcccccgcc cgcgcgccct ggtggcgcgt cctgtacatc caggttctcc tggccgtcgc
     7381 cgccggcatt ctgctggggt ggcttttccc cgacaccggc aagactttga agccgctggg
     7441 ggacggcttc atcaagctga tcaagatgat gatcgccccg gtcatcttct gcaccgtcgt
     7501 gcatggcgtg gcttcgatgg gtgatttgaa aaagctcggc cgggtcggtg gaaagaccct
     7561 cctgtatttc gaagtcgtct ccaccctggc cctcgtcatc ggtcttgtcg tcgtgaacct
     7621 gctcaagcca ggcgcgggat tcaatgtgga ggtggccgac ctcggcgcca aagcggccga
     7681 cgccgcgcgc gagtatgccg ggaaggcgca gggcctgagc accgtggatt ttctgctcgg
     7741 catcattccc gggacgttcg tcggcgcctt tgcgggcggt gatcttttgc aggtgctctt
     7801 catcgccctg ctcgccgcgt tcggccttgc cggcatgggg gagcgcggca agtccctgct
     7861 gcatggcatt gaccgagtca gcgaggtctt ctttgccatc atgcgcatcg tggtgaaagc
     7921 cgccccggtc ggcgccttcg gcgccatggc cttcaccatt ggcagctacg gccttgagtc
     7981 cctgcaaagg ctcctcgccc tcatgctggg cttctatatc accgccgggt ttttcgtcgt
     8041 cgtggtgctc ggcctcatcg cgcgctggtg cggcttttcc atcttccgtt ttctggccta
     8101 tatcaaggag gagcttttgc tcgttcttgg caccagctcc tcggagacgg cgctcccgtt
     8161 gatgatccaa aaactgcgcg gcctgggctg tgctcccgcc acggtgggcc ttgtggtgcc
     8221 tacgggctac agtttcaacc tggatggtac gaacatctac atggccatgg ccgcggtctt
     8281 cctggcccag gccacgaaca cgccgctgga ccttggccag caggtcagcc tgctgctggt
     8341 ggccatgatc acctccaaag gcgccagtgg tgtcacagga gcgggattca ttaccctggc
     8401 ggccactctt gccgtcgtgc ccggcatccc catcgagtcc ctggcgctgc tcgtcggcat
     8461 cgaccgtttc atgagcgaat gccgcgccct gaccaatctc atcggcaacg gcgtcgccac
     8521 cgtggccatc agccgctggg agggcgaggt caccccggag gtcatcaatg cgaatctgcg
     8581 cgacccagtt cgcctgccgc cgcccgcatg aacagaggac gcagtccgcg cgcggattcc
     8641 catgagaagc ggccggtgtc tgagaagttt tgatttctct ttggcctcat gggcgttatg
     8701 acccttgaag acttccccgc ctcactcatc tgcatgattt tttccctctc ctcgcgcatc
     8761 ctcacgactg tggatccgaa atgcctggcc aagatcgcct ggaacttcgg tttcaaaggc
     8821 gcgcgctcgg tgatgctttt caaaaagcgc atggagcagg gccagttctt cccgcctttc
     8881 ctttacatct ccatcctgaa ctcctgcaac ctgcgctgcc agggctgctg ggtggatgtg
     8941 gacaagccgc gcgaggccat ctctctggac gagctgaaca agatcatcaa cgacgccaaa
     9001 aggcacggga acgccttttt tggcctgctg ggcggggagc ccttcatgca cccggaactg
     9061 ctggacctgt tggcgctgca cccggactgc tactttcaaa tcttcaccaa cggccagctc
     9121 atcaccgcca agaccgccga ggcgctgcgc aagctgggga atgccacgcc gctcgtcagc
     9181 gtggagggca acagcactgt gagcgacgag cgccggggca acaaggatgt gctcaaccgc
     9241 accctgcgcg gcctgcgcaa ctgcctggac gcgcgcctgc tcaccggcgt ggccaccagc
     9301 ctgtgccaga cgaacattgg cgatctgctc acgcgcgagt ggctggagaa gctggtctcg
     9361 atgggcgtcc actacacctg gtaccacacc taccgcccgg tgggtccgca gatcaatgag
     9421 cagctcgccc tgacaccggc gcagatcacg gaggtgcggc gctttgtcac ctccatgcgc
     9481 gcgcgggtgc ccatcgccat cgtggacgcc tattacgatc acaatggcaa ggcgctctgc
     9541 cccatggcca cgggcatcag tcaccacatc gggccgacgg gcggcatcga gccctgcccg
     9601 atcatccaat tcgccaagga gacggtgcgc acggaacggg acatctttga cgtgttcacg
     9661 cagtcggagt ttttgaagga cttccgcgag acggcggcgc ggcacacgcg cggctgcatc
     9721 gtgctggagc ggcccgacct ggtgaaggag ctggcgctgc gccacggcgc caaagacacc
     9781 acgatccgcc agacggcgat ggccgaggtg gagagcatga agccgcgcac cagccagtgg
     9841 cgcgaggagg aggagatccc cgagcagcac tggatgtaca aggtggcgaa gcgctttttt
     9901 taccacgact tcggcgtgta tgaaaaaatg gacccgaagg cgcactcgcc cgccgcgtga
     9961 ggcggagctt gcgcattgca tgacggcgga cacttttctc ccatgagcac cctccttgag
    10021 caaatccgcg cactgccggt ggacgagcag atggcgctgg ctggcgacgc cctggaatcc
    10081 agtggcgagg gaaatgattc ccccggtctc gcgccaagtt tgcaggtgga gctggagcgt
    10141 cgcattgacg agcatctcgc gcatcccggc agagcggcga ctttggaagc ggcaaggcag
    10201 cgtaacaatg agagcgttga gtctcgcgca gcataaaagg cgtgatgctt tttgagaact
    10261 gcattcacaa caaggccatg tcttttccgc cacgcgctgt caccatcctc atgttgtgcc
    10321 agatttttat tgtcacggga ggacttctgc tcacgcgcag ttttcgaaag ctccatgaca
    10381 aaactcttgg cgagactttt ggggacaagc ttcttccgtc attctccaac cttgcgatga
    10441 cctggggatg gctgctgctt ctaatcccga tgtgctggac attatattgt ctccgagctt
    10501 caacagggca cggcacgaac ggcgaggtcc gaagaatgga tttttggatt ggcctgtgcg
    10561 tcctcgcagg acttttttgg atgtgcagtt tttcggttct ggcccatatg aacgcactgc
    10621 agacaaggtc tttctagatt cgatgcccga cacccccaac ctcctctatt gccgctgcgc
    10681 gtacgcccag gtcgtgccgg ccggcgtgaa aaatgaagtg ctccagcgcc tgtgcgattc
    10741 cggcgccagc tttgaaaccg tgtccgatct ctgcgagatg gccgcgcatc gtgacccgcg
    10801 tcttcaggaa ctcgtggccg cgggcaggct gcgcatcgcc gcctgctatc cgcgcgccgt
    10861 tcacggcctc ttccatcaag ccggcgcgcc tttgccggag gacaccgaga tcctcaacat
    10921 gcgcacccag ggcgcggagg aggtggcggc gaagatgctg cgcggggaga attgatgcgc
    10981 atgaaatgaa aaccaaatca cacccggctt atcatcctgt cagggcggtg ttgctgcctg
    11041 ccattatgat gccaatgccg gcgatggctc gtcttccccg gcggcgtcct ccacgggaat
    11101 cccatccggg tgatgcgcct cctcggcggc cacttcggct tcaagacgga gctgctcggc
    11161 tttggagggc tttttctcct cctgggtcat gcgcactccc accggcttgc tgacgccgaa
    11221 ttcctgcatg gtgacgccgt agatgacatc ggcgcggccc atggtgcgct tgttgtgggt
    11281 gacgatgatg aactgcgagt tctcgatgaa gttgtccagc atcttcaaga agcgggaaat
    11341 gttcgactcg tcgagcggcg cgtccagctc gtccagcacg cagaaggggc tgggcttcac
    11401 ctggtagatg gagaagagca gggccacggc tgtcatgctg cgttcgccgc cggaaagcag
    11461 ggatatggtt tgcagcttct tgcccggcgg tttggcgatg atttcgatgc cggactcgag
    11521 cggatcggtc tcgtccacga gcatgagatc cgccttggcc tgggggccga agagctcttt
    11581 gaaattgttt tggaagaagt tgcgcacgag ctggaaggtc tcggcgaaca tggtcttcgt
    11641 ggtctcgttg attttggcaa tgacttgaag cagctcctcc ttggatttga cgaggtcgcc
    11701 atgctgggtt tcaaggaaat tgtggcgttc ttccagctcc tcgaactcct ggatggcatc
    11761 cagattaacc gggccgatgc tttcgagttt ttgccggagg tcgccaacaa tgctggtgac
    11821 gaattcccag tcgggctgcc cttcctctcc ggggatctcg atctcgtcga ggtccacgat
    11881 ttcgtcgtct tcgagggctg tgcaggtggc gccgggagcg ttggacaggt cggatgagtc
    11941 ggacacttcg ggctgaattg aaggctcagc agtctcttgc gcctccgatt cttcgacttg
    12001 tcctggtgtt tgctcaaccg tggcctgggt ttccatttcc agggtggcct tgcgcttttc
    12061 cacgcggctg cggccttttt tctgctcggt gatgctgagc atgagcgcat ggtagtcggg
    12121 ctcgaaggcg gagatgtgga agctgtagcg ctcctgcacc tggttgacga ggttttccag
    12181 gcgcaagtcc acgcgggtga gctgcacttc caggcggctg cgggactcgc tgaggccgga
    12241 gatctgctgg cgcaattgca caagctggtt ttccagcagg ctcacgcgct caaaggcggc
    12301 tgcgcgctcc tcggtgcggc cctgcaggtg ctcttcgatg gctgcgatgg ccccgcgctg
    12361 ggattcgatc tgctcggcga agcgggcgct ctccgccgcg ctctgctcga tgcgggagcg
    12421 ccaggtgcat atttcctggt caaagcgctg gatggaggac tccagctcgt gcaggcgcgc
    12481 ggccatgggg gccttctggc ggttgaggga attcagcgcg ctttcctcca aagcgagggc
    12541 ggtgcgcagt tcgttgagct tctccgagga ttcgagttcg cggcggagaa aggactccat
    12601 ctcaagctcc agctcgccct catgcatgcg cgcgccctcg agttgctcct gggccagccc
    12661 tgcctgctcg cgctgccagg tgatctgcgc ctcggcggac tggatgcggc tggtgatctg
    12721 gttctgatcc cactcgatgc tttccagtct cgtcgttgcc tgctgcagcg cgcgctgcac
    12781 cacggagagc tgcccttgaa gctgggagaa gccctcgcgc gcgcgctggc tctgctcgcg
    12841 cagggagatt tcctcgcgct gcgcctcctc aagctgggcg cgcagttgct ccacggcctc
    12901 ctccttttcg agaagctggt actcgaagtt ctccagctcc gctttcagag tgcctacttc
    12961 ggactgccgc cgcagcatgg aggcggcctc ctctttggag gcgccaccgt gaatgatgcc
    13021 gtccgcgccg agcagctcgc ctttcaatgt gacgatggcc gcgtcgggaa gctgccgctt
    13081 catgcgcagc gcggtgtgca gatcctcgac gatgagcaca ttgctcagca gacggtcggt
    13141 gatctcctgc gcgccgggtc gggcgcgcac tttttccacc gcccaggcga tggagccgcc
    13201 tgggacgagc tggcggtctg gcacggcgcg cagggtggcg aggtcttgtg gaatgagcgc
    13261 tgccctgccc aactgatgat tcgacaggcg ctcgaggatc tgcgatgcaa gctcgctgtc
    13321 ggtgagcaac acggcctgca aatgatcccg cagcgcggct tcgatggcgg caatgaactg
    13381 cggctcgact tcgatggcgg aggccagcag accgcgcacg ccgatggaat aaatctctgg
    13441 attgtccatg cctttgagca cctgctgggt gccttcggcc aggccctcgc ccttttgcaa
    13501 cagctgctcc aggatttcga cgcgcgaacg gcgctgggtg acggcgcgct ggagctcgtt
    13561 caattcctca ttgagcgcgt cgcggcgggc gcgcttgtcg aggatgtcgc gggcgcattg
    13621 cttggacctg tcctccagct cgttgcgggt ctgctccaga tcatgaatcc ggtgctggat
    13681 ttcatcaaac tcgatctggc tggattcgcg agcctgggcg gcggcctggt ggtcgtgggc
    13741 cagggtctcg tgacgctggc ggtcggcact gatctggttg gtgaggctct gcgcgcgcgc
    13801 gtctgcggag gcgatctgac cctcaaactg gcggatggac tcgcgcaccg cgcggcgggc
    13861 ctcgtccagg cggccgcggt cgggcaggat ggcattgtgg acgctcaagt gctcatgcac
    13921 ggcgttgcgg cgcgtctcca gattcgcgct gatggccagc acctgctcgt cggcgtcggc
    13981 catctcacgg cgctgctgct cgagcaactg ctgtccctgc ccgatctgct cctcattttg
    14041 acggatgcgc ccttggagtt cctcgttgcg ctcgttgttg aagccgatgc ggccctccgc
    14101 gctctgcacc tggctgcgca gctcctgcgc ctgctggcgg agctggttga tctgcgactc
    14161 cacggcgtga taggcctcgc gcgtctgggc ggcctcggtt tcggtggtct gcagcgtgtt
    14221 ctgcaaagcg tcgagctgcc cggtcagcat gcgcacatga ttttccgatt cggccttctc
    14281 ggcgctgaac tcgctgtggt gcttgtgcgc gaggtgggtg tcgaacatgc gcacgtcatc
    14341 aagcaaagcc tggtaacgcc gcgccttggc cgcctggcgt tggagagaac ccatctggcg
    14401 cttcacctcc gcgatgacat ccgccacgcg cagcaggttg gcctcggtgt agtcgagctt
    14461 gcgcagggcc tcttttttct ggcccttgaa tttggtgatg cctgccgcct cctcgaagac
    14521 ggtgcgccgg tcctcgggct tcgatgagag cagcatgtca atctggccct gcgccatgat
    14581 cgagtatgcc tgccgtccga tgccagtgcc ggagaaaagc tcgtggatgt ccttgagccg
    14641 gcacacggtg ccattgaggc ggtattcgct gcgcccgtcg cggaagacgc ggcggcagat
    14701 ggccacctcg ttgtaatcca cattcagggc ctgctcgcag tccgccatgg tcagcaccac
    14761 ctcggccatg ccgacgggtt tgcgcttgtc ggtgccatta aaaatgacat ccgccatttc
    14821 atcaccgcgc agggccttgg cggaggtttc gccgagcacc cagcggatgg cgtccacgac
    14881 gttcgacttg ccgcagccgt tggggccgac gatgccggtc acgcctttgt gaaattcaaa
    14941 gtgggtcttg tccgcgaagg acttgaagcc gtgcagggtg aggctcttga ggtacatggt
    15001 ggctggtaaa acgaattctg gcagtatcca cgatcagagg ccatgaaggc aagagcgaag
    15061 ccattgtttt cccatatctt gggtttgctt cgtatgtctt aaacaagatg tgcgattttc
    15121 tgcctgtgaa atactgtgaa caattcgctc tcttgtgaag aaacccggat cgagggtcag
    15181 gccagaggcg ggcaatagtt cagcaactcg atttcaaagg cgtgtttttt ttccggctgg
    15241 atctccacca cgctgatgct gccatattca atattgaagt gcgccatgtg tttgagcggc
    15301 tgaccaagca gcagcgcgag gatgacgcgg atgatgccgc cgtggcagaa gacagccacg
    15361 cggcggtgtg gattgtcgtg gaggattttt tccagcgctg gccgcactcg gtgggcgagc
    15421 tcgtggccgg actcgccgtt gggtatgccg gcctcctcga tgatctccag ccagtcaaag
    15481 gcgctgacgc cgaagtgctc cttgactccc tcccagcggt atccggtcca gtcgccaaaa
    15541 tccacctcgc gcaggctggg gatgatgacc ggcttcaaac cgctggcctg ggccagcggc
    15601 gccagagttt cctgcacgcg cagcaggggg ctggcgtaga tgccgtccag cgaagtgtct
    15661 gccagccagg cggctaccgc gcgcgcctgg cgtcgtccga gatcagacag ccccatgtca
    15721 atgcggctgc cgccgaagac tttgtggtag cgctcctcca cctcgccgtg gcggatgagg
    15781 tagagctggg tggggtgatt gggaaggtgg ggtttcaagt cagcgcttcc atgctgcatg
    15841 gagcgcgcgc gcgcaacccg cgagccgtgg tcaaaaagtc ccgccagctc aaagctgcaa
    15901 gccgtgcgct gggccgcgcg ttttctccgc gccatgacca accgccgccg cttcctcgtc
    15961 cagtccgccg ccgcctccac cggcgttttt tacatcgcca aaacctcctg ggcgcaaaaa
    16021 agcccgggcg acaccctgaa tgtggccgtc gtcggattcg gagggcgggg cggctcccac
    16081 atcagcggat acaaaaagct cgacaaggag ggcgtgcggg ttgccgcgct ttgtgatgtg
    16141 gactcgaagg tgctcgaccg cggcgttgac gcctttgaca aggagggcaa aaaagtcgcg
    16201 cgccacactg acatccgccg tctgcttgag aacaaggaca ttgacgccgt cagcatcgcc
    16261 acgccaaacc actggcacgc gctgggcacc atctgggccg tgcaggcggg caaggatgtg
    16321 tatgtggaaa aacccgtctc gcattgcgtg tgggagggcc ggcagatggt gaaggcggcg
    16381 cgcaaatact cgcgcatcgt gcagaccggc acgcagtccc gctccagccg caagggcatt
    16441 tatgaggcgg tgaaatgggt gcgcgcgggc aatcttggca agatcaacgt cgtgcgcggc
    16501 ctctgctaca aacggcgcga cagcatcggc aaggcggagg gcgcgcagcc cgtgccggag
    16561 actgtggact acgacctctg gtgcggcccc ggcccgctgg cggacctcac gcgcaagcgc
    16621 ctgcactatg actggcactg gtcctgggcc tacggcaacg gcgacctcgg caaccagggc
    16681 atccaccaga tggacatcgc ccgctggttt ctcggtgaga tggagctttc gccactggtg
    16741 tggagcatcg gcggacggct cggctacgag gacgatggcg agacggcgaa cacgcagttc
    16801 atttaccacg gctatgaaaa agccccgctc attttcgaag tgcgcggcct gccgcaggcc
    16861 gccggcgtga aggacatgga taaatacaaa ggcgcgggag tcggtgtcat cgtcgaatgc
    16921 gaaaaggggt atgtcgtcgt gcccagctac agcaaggcca cggcctatga cctgaaggat
    16981 cagatgattc tggagtggtc gggatcggag gatcacttcg ccaatttcat caacgcctgc
    17041 cgtagccgcc gggtggacga ccttcatgcc gacatcctgg aagggcacct ctccagcgcg
    17101 ctctgccaca caggcaacat cagccaccgg ctcggagcga aggagaagcc ggaggccatt
    17161 ctggaaagag taaaggatta ccggaccggc acggagacct tcgagcgcat gaaggagcac
    17221 ttggcgaaga acggcgtgga catctccaaa gacctgctca cgctcggccc cgtgctgggg
    17281 atgaatccgg agaccgagcg cttcaccggc aacgacgcgg cggacgccat gctcaagacc
    17341 aactaccgcg agccttacgt cgtgccggag atcgtctgaa cgctcacttc ttcaaagcct
    17401 cgtcgagcag cacctcgagc gcccagcggc gcagccacgc atcgtcggga gcggggtttt
    17461 gtccgggagg ctgcgcggat ggacgcgagc gtccctccac tggcggaccc gtcacggcga
    17521 tgatccagcc gccatccggg gtggctgccg cgccggggtc cgctccgctg acgcccggtg
    17581 actcgatcag ccgccactcc tgtccgtcgc tgctcacggc catgaaaatg ccgccgcgtg
    17641 gagatcctcc gtccgcgcgc ggatcgccgc cgccgtcgct ggtgccgaaa aaggtgatcg
    17701 tcgcgccatt ggactgagca ttgcccagcc agcgcagcct gcccggtagc gacacatcct
    17761 ccgcacgggt gaagttcagc ccgtccgtgc tcgtggcgtg gaagccggct ccctgtctcg
    17821 gtcgctcctc gccgccattt tcagggcgcc cgccattgtc cggcgcgtaa aggtggaaca
    17881 cgccctggtg cagcaccacc gcgcagtcaa tgacgggccg gttttcgatc tcaaagcgcg
    17941 cgccttcctc gtagcggtag ttcacgccat cctcggagat ggcggagtgg atgcgcgggc
    18001 ggttttccga aaagacccgc ccggtcattc cggtgaagta aagccgcacg cgcccgtcag
    18061 gcagcgtgac cagcgtggga tcaaacggga agcgcatccc ctccggcagc ccttcgactt
    18121 gcatgacccg cgcgggtgtc cacgagaggc cctcgtcgga ggaaaaatgc accgcgatct
    18181 tgtcaaagtt ccccgggtca ttcttcggga agtgctggtg cgccgcgagc aggcggccat
    18241 cacgcagcct tgtgagggtg ggcacgcccg cgcgctcgaa gacggcgact tgcggcgcgc
    18301 cgccatcctt gccgagttga tgcaccagca catcattctc ccaggggccg cgctgctgcc
    18361 ggccggggcg ttccggctgg ccgggctgcc tgtttggcat gggaagcagg gggtcgaaca
    18421 agacatccag ggcactttga gccggagcgc tcagggcggc ggcggagcac aggcagagca
    18481 ggcggagact ggcgcgggtg ttcatggcgg gaggcgcggg ttggaaggga ggcatgacgc
    18541 cgctccctcg gcttaacgcc gccggagagg gtttgcgtgt gtgccgcgtc tattttttca
    18601 aggatgcccg gcccgtgccg ccacaaactg gaagactttg cgctccctgc aaaacttgcc
    18661 tcttggagat cggcggcgtt gtgcgtagtt gcgcgcgccc cccatgcaac ccgccgcagc
    18721 ggccagccag accggccgcc tacccctggt ctctcttttc tttttcaccg ccatgcccct
    18781 gggcatgtgg aatgtgccgc tggccaacgt cttcgccgcg cacgggcggg cgcaccttgt
    18841 gccttatgtg ctggccacat cggcggtggc ggccttcatt tcgcctctgt tcgtcggcgc
    18901 gctggcggat caaagacttt ctcccaccac tctgctgcgc tggctgtgcc tggggacggg
    18961 cgcgggcctc gcgctcatct gcaccgccct ggcgcgcggc atgaacgacg gcatgatcct
    19021 gctctgcgcg caggcgctgt cactggtctc cgcgccgctc tggggggtga ccacgagcat
    19081 cgtgcttgcg cagttgaggg agccaaagcg cgagttcggc cccatccgcg cctgcgccac
    19141 ctttggctgg atgggcggct gctgggtggt gagttttgtg ctcaaggcgg acaactcgct
    19201 cgtggcgggg tattcggctg cggcgttgtg gctgggggtg gcggccttga cgtggacact
    19261 gccgaagaca gcgcccgcgg atgtccaggc tggcaggaac tggcgccagg tgctcggcct
    19321 cgacgcgctg gacctgctga aaaaccgcga ccaccgcgtg gtcttcatca ccgccgcgct
    19381 ttacagcatc ccgctggcgg cgttttaccc ctacacggcg cggcagctcc atgacctgga
    19441 ggtggagagc gtctcggcgg tgctctccct ggctcagacg acggagatcc tgaccatgct
    19501 gctgctggcg aaggtgctgg cgcgcgcgcg gctcaagtgg gtgtttctcg gcggcatcgc
    19561 catctgcgtg ggccgctatg ccttgaacgc gctcgacacg cggggctggg tcatggcggg
    19621 caccacgctg cacggattcg ccttcacgct ctacttcatc accgcgcaga tttacctgga
    19681 ggagcgcatt gacccgaaat ggcgtgtgcg cgcccaggcg ctgctgaccc tgctcatggg
    19741 cggagtgggg aatctgctcg gctacctcgg cggcggctgg tggcacggtt tatgtgtcaa
    19801 agacggcgtc accgactggc agcgcttctg gcttggcgag acggcgctca cagcagccgt
    19861 gtgcgtcttc ttcgcgctgg cgtatcgcgg caggggcgcg cgggcatgaa aaaacccgaa
    19921 gccggctgaa ggtccggact tcggggtttt ccaggcggca atcaactagc gagcactttc
    19981 tccacgcccg tgacgatctc gcggatgcgg aagttgaggc cgtcggtctt gcagagggac
    20041 tggattttcg ggtcggcgag cttggcgcgc agcagcatgg cgagctggcc gtagccataa
    20101 acaccgacgt cgttgagctc gacgacgacg acatttttga atttggcgaa gatgccgtca
    20161 agccgcgcgg gcatggggtg caggtggcgc aggttgatgg cgccgacctt gtggccctca
    20221 tcgcgcaaac gattcacgct ttccttgatg gggccgtagc tgctgcccca gccgacgagc
    20281 aggacatctc cttcctgctc gccgtagatc tccggcagcg gcagcttttc ggcgagttcg
    20341 acgagcttgt tgcggcgctt gtcggtcatc ttctggtgca tcttcggatt gccgctcggg
    20401 tggccccatt cgtcatgctc caggccggtg acgtgcgggt agcggccgcc gagcatcttt
    20461 gcgcccggag gggcgtggcg ggtgatcttg tcgagcgggt agggtttgaa atcctcgggc
    20521 cggtcggaga gatcgagcac gggctccacc cagtgcttgt cgaggtcggg ctcggtgaag
    20581 gcctcgatgc ggctgctgag cgcctggtcg ctgaggatga tgacggggca ggagtactcg
    20641 cgggcgagct tgccggcctc cagcgcgatg tagaagcagt cctccacgtc ttgcggcgcg
    20701 agcacgatgc gcgggcagtc gccgtggctg ccgtagatgg cctggagcag gtcggactgc
    20761 tcgacgctcg ttggcagtcc ggtggagggg ccgccgcgct ggacattgat gacgatgagc
    20821 ggcagctcgg ccatgctggc gtagctgagc gcctccatct tcaggctgag gccggggccg
    20881 gcgctgccgg tgacggcgag gtggccggcg aaggccgcgc cgatggtcat ggacacggcg
    20941 gaaagctcgt cctcggcctg cacatagatg ccgccgtatt taggcagctc ggcgcggagc
    21001 tgctccatga tgggggacca gggtgtgatg gggtaggccg cgccgtagcg gacgccggcg
    21061 cagatgaggc ccatggtcat caaggtgttg ccgtccgtgc tgaggcggtg ctcgtccttg
    21121 tgctcgccct cgtcgaaatg gaagctgccg atgtcaccca ccggataggc ataacctgcg
    21181 tcgaaggcga gcatggcgtt gcgcagcacg ctctcgtcct ttttgccgaa ctggcgggcg
    21241 atgatgccgg agagcttctc gcggtcgagc tggaagacgg cggtgagcag gccgagcacg
    21301 aacatgtttt tgccgcggtc gcgcgcgctg ccgccgatgg cctcgatggt ggcggcgttg
    21361 atggggatgc ctatgtggtg gatgccgcgc tcgcttttca cctcggccac ctcgccggag
    21421 tcataaatgc agatgccgcc gtcgcgcagg gcgtgcaggt ggtcgttgta actgtgctgg
    21481 taaaaggcca cgagcacatc gctctcatca ccgggatgca gcacctcgcc ggagccgagg
    21541 tggagctgga agatggaggg gccgccggag atggtggagg ggatggtcat gtaggtcatg
    21601 acatccttgg ccgtgcgccc ggcgagtcgc gcgaggaagc cgccgatgga ttgaatgccg
    21661 tcctgggagt tgccggccag gcgtatgacc gcgttgcgaa gcgtcttcgt ggcggacgct
    21721 gtgggggttg gggtggttga catggaaaag ttcgaggccg gagaaaacgc cgggttgggc
    21781 gcgctttttc ctcggctgcg tagtaggcgg gcggcggcgg cggtcaaaag ggaagttcga
    21841 atttcacttc ttcggctcgg gcggcaactg cttcgggtga ccttcgagcg caacggggga
    21901 ggtcttgtcc tcggtgg
//
LOCUS       DAOMYJ010000072        21665 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Prosthecobacter sp. isolate AMR_MDS_5629
            NODE_858_length_21665_cov_20.409718, whole genome shotgun sequence.
ACCESSION   DAOMYJ010000072 DAOMYJ010000000
VERSION     DAOMYJ010000072.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867850
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prosthecobacter sp. (wastewater metagenome)
  ORGANISM  Prosthecobacter sp.
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Verrucomicrobiaceae; Prosthecobacter.
REFERENCE   1  (bases 1 to 21665)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 21665)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.30
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:34:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,742
            CDSs (total)                      :: 4,684
            Genes (coding)                    :: 4,676
            CDSs (with protein)               :: 4,676
            Genes (RNA)                       :: 58
            tRNAs                             :: 55
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21665
                     /organism="Prosthecobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_858_length_21665_cov_20.409718"
                     /isolate="AMR_MDS_5629"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1965333"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(119..1321)
                     /gene="mraY"
                     /locus_tag="PLP58_09565"
     CDS             complement(119..1321)
                     /gene="mraY"
                     /locus_tag="PLP58_09565"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009962787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008963 -
                     phospho-N-acetylmuramoyl-pentapeptide-transferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HRJ08805.1"
                     /translation="MIYWLYELRNWLEAGQWITVDSLVYKVLNIFRYHTFRAGGAAVT
                     AFFLSVFFGGWLIRKLISLKIGQPVRTAEEVNKLYELHGKKAGTPTMGGILIVGAILV
                     STLVWAKWDNLMVWIILFATLGLGALGFWDDYLKISKKNSKGVSARAKLVWQGGVAFA
                     AGLAFCYLMPPDVLVDQASKAGQDAPILHMGGTETYRALYVPFLKEPLIADMGFFAVL
                     LFTLVIVGASNAVNLTDGLDGLATGCSLTTALAYMVFGYVCGNANYSEYLGVAHHSLA
                     NELPIIAMSLAGACLGFLWFNAHPARMFMGDTGSLALGGCIATLAIGCKQEIVLVLVG
                     GVFVMEAASVMIQVASFKTRGKRVFRMSPIHHHFELGGWHENQVIVRFWILSILFALM
                     GLASLKLR"
     gene            complement(1318..2703)
                     /gene="murF"
                     /locus_tag="PLP58_09570"
     CDS             complement(1318..2703)
                     /gene="murF"
                     /locus_tag="PLP58_09570"
                     /EC_number="6.3.2.10"
                     /inference="COORDINATES: protein motif:HMM:TIGR01143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0047480 -
                     UDP-N-acetylmuramoyl-tripeptide-D-alanyl-D-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HRJ08806.1"
                     /translation="MMKAIPLQTLLSFCPGIVRRAGDSTRAVTRVVTDSRAVQPGDVF
                     VALKGERFDAHDFIPQAAASGASAVIVSHVDEKWSLPEHCALMEAPDTLTALQNLARG
                     YRSWHKPLLIGLTGSNGKTTTKDFAAAVMSAKFQTRATAGNLNNHIGVPLTLLSLQDG
                     DECAVVEMGMNHPGEIRTLVDTALPDAAILTNVGIAHIEHMGSRDAIAWEKGTLPANV
                     PGGGTVVLPADDDYTPLIARHCQARVLTAGIESGDVSASELERVENGTRFKLDFRGES
                     AEVFLPVIGRHMVVNAALAACMGWAQGIAPEDIAAALSRACITGGRMQVLDIGGVVFI
                     NDSYNANPDSVMAGILALRTTGKGRKHAVLGRMGELGARAEEEHARIGRSAASTLDSV
                     ITVGDGEARLISQAAADTESFDPCHTAHFATHEECAAHLRQLVKPGDAVLVKGSRSTR
                     MERVLELYQQS"
     gene            complement(2704..4260)
                     /locus_tag="PLP58_09575"
     CDS             complement(2704..4260)
                     /locus_tag="PLP58_09575"
                     /EC_number="6.3.2.13"
                     /inference="COORDINATES: protein motif:HMM:NF001124.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HRJ08807.1"
                     /translation="MKLRDIVARLEQPVVSGSLDTDIRALAYDSRKAGPGSAFFALRG
                     AQADGHDFIQAAMTAGAAAIFAETAPPADAVIPWVHARSMRRALAAAAAALHDDPGRV
                     LALAGITGTNGKTTTAFLLHDLLNQGQKRAGLIGTVSWDTGARKPKPATHTTPESLEI
                     QGLLSEMRDNGCRTCVMEVSSHALDQERVHGLDFAAGIFTNLTQDHLDYHGTMEKYFA
                     AKARLFEKIAAQKTGRMVVNGDDAWGRKLITRFEGTGKVVRYGFGAGLEFRATNVRYD
                     VTGTTYELEALGRHLLVRLPLIGDFNVYNSLAALAGAHAMGLNLRESVAHLKSAPQVP
                     GRLERVTDQTRFHVYVDYAHTPDAIENALRTLRALRPRRIITVFGCGGDRDRTKRPKM
                     AAAAEAGSDVCVLTSDNPRFEDPEAIIADTIKGFARPKSHAAIADRRQAIRAALEHAQ
                     GGDIVLVAGKGHEDYQDIQGKKHPFDDRRVVRQTLNDLFHERDAEAAEREALREQEMR
                     DQDMRGPWRD"
     gene            complement(4257..6098)
                     /locus_tag="PLP58_09580"
     CDS             complement(4257..6098)
                     /locus_tag="PLP58_09580"
                     /inference="COORDINATES: protein motif:HMM:NF013100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HRJ08808.1"
                     /translation="MILAASLLVTGFSAVVWRLYDLHVRQAPRLAELAAAKFREERVL
                     LARRGTIKDFSGRLLAYDEQVHELRTNQVHLHELPTIKPCLAAVKGMTVRELTLKMSD
                     RAIFDAYHDHVSRALAAKLGRQVPEMLAVVKSKEPVQTLVREMDDEKSAEWAGYLEAM
                     QIKGVYVKPAVKRSYPAGNSMALIVGGTAPGKGGTYGIEKGRESILHGENGSVQIERN
                     KRGEEMLLYRGEMIDPRHGSGLHLTLDLALQEAVDKILIEAHNRWKATRSMAVVTEVT
                     TGTVLAMSSLPAHDRALAGQSEATNWEHFAVNKPYEPGSTYKIVAFTAALDQRKITVD
                     ERFDCHWGKYHDPVLNFDLEDLTKMGTVPARDVFTHSSNIGTYKIFKKTGQAVFLDYT
                     RRFGFGSATGCGIPGESAGKINWSNWSNTTYSSFPMGYEVEVTPLQMALAMGALANNG
                     LLMKPRLVDRVTSERGVEHLQPEVAGQVCSGRTAQMMRELLKNVVAKGTGKAAEIEGI
                     EVAGKTGTAQRFDPEMVTGYRKDGTPKKGGYRKDQWVVSFAGFAPANDPKIACVVVLD
                     NPKKSLDTDVIGGGAVAGPVFARIVEETLKQLSVRPQRPLALKGGGQ"
     gene            complement(6152..6499)
                     /locus_tag="PLP58_09585"
     CDS             complement(6152..6499)
                     /locus_tag="PLP58_09585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ08809.1"
                     /translation="MKNRRNSSGLSLPRLSALLTIIVLLLLTALSVVVNKNRITALAD
                     QQRLVEQETRLLKFEITNLDNKIGTQLLDKKRVSGVLADKALQLQEINKDARSVIYLK
                     SASIPVAQNSSPQ"
     gene            complement(6496..7515)
                     /gene="rsmH"
                     /locus_tag="PLP58_09590"
     CDS             complement(6496..7515)
                     /gene="rsmH"
                     /locus_tag="PLP58_09590"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: protein motif:HMM:NF013919.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016434 - rRNA (cytosine)
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HRJ08810.1"
                     /translation="MPWGGGKCVSRPQGGDHEPESQPPHDSEGGRQTPFYHLPVLTQQ
                     VLDFLQPRSGKLFLDGTVGGGGHAEALLQHGARVVGMDQDEEALDHVKRRLACYEDRF
                     CALRGNFRDFPKVLGEAGVSGLDGILVDIGVSSRQLDSAERGFSFQKDGPLDMRMDRG
                     ASLTAAHVVNGYELENLARVIREFGGEPHAMRIARAIVRERARQPFTSTLQLAELIER
                     ACPGRGRKHPATLTFQALRIEVNQEIAALREFLQEAPRWLKPGGRLVVISFHSTEDRE
                     TKQAFQRMSAPFLDKKEWASPRPNPDCLLRVLTRKPVEAAEAEVTLNPRARSARLRAA
                     ERLPA"
     gene            7526..7738
                     /locus_tag="PLP58_09595"
     CDS             7526..7738
                     /locus_tag="PLP58_09595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ08811.1"
                     /translation="MASEAPNHGHTPTWKNSKKRGCNAELRCPFPRRISFFWKILQKL
                     EKMFSVDDVMADLRESSLEAKVSVDN"
     gene            7916..10381
                     /locus_tag="PLP58_09600"
     CDS             7916..10381
                     /locus_tag="PLP58_09600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018969588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3656 domain-containing protein"
                     /protein_id="HRJ08812.1"
                     /translation="MIQSHLPELLSPAGNWDCARAAVANGADAIYFGLPRFNARMRAD
                     NFTAEDLPELMRFLHRHGVKGYCAFNVLIFTRELPDAAGQLQLLEQSGVDAVIVQDIG
                     LARLVKALTPRLRLHASTQMTLTSPEGLELARTLDIDQAVLARELSLRELERFHAEEN
                     PALPLEVFVHGALCVAYSGQCLTSESLGQRSANRGECAQACRMPYEMLVDGEPRDLGD
                     KRYLLSPQDLAAVREIPRLIELGIRSFKIEGRLKTPEYVAAVTRVYRKAIDSARAAPG
                     APAAFTEDDRYELEMTFSRGLYSGWMHGVNHQELVGAIYGKKRGMPAGVVRSTARDAV
                     ELESVPAHLKTGDGLAFENLHDTNDEQGGRVYGIRGRWIEFQRGRLDTSRIPPGTRVF
                     KTGDQVLEQRLRQTWQGEIPQRRKTRLDLAVSGKAGAPLLLECDAPRARVESATALQP
                     AERSPLTREILQNQLGRLGATTYELGSLDMRVEGAVMLPVSELNRMRRALVGQVSQNS
                     EAPAAAPSSAPRSSLDEMLAAVSAMRAPAGDEPPRLHVLCRTFEHVQAALDSGVTRLY
                     ADFEDIRLYGDVVGRVRAQGGASVFLATPRIQKSGEAGFFKLIARAQPCGVLIRNLGG
                     IAFFKDAGLPAIGDFSLNTANPLTAGFLKDTGLGRLTISYDLTIEQVLDLLRSAPPQW
                     FEITLHQHMPMFHMEHCAFAAFLSEGTDFTNCGRPCERHRVHLRDRVGMEHPLKADVG
                     CRNTLFNAVPQTGAQFFADLMAAGLRDYRVELLEEDAAEARRVISAYQDLLSGSSGGD
                     IWRALKARSQIGVTRGTMAGRSF"
     gene            complement(10403..10900)
                     /gene="rsfS"
                     /locus_tag="PLP58_09605"
     CDS             complement(10403..10900)
                     /gene="rsfS"
                     /locus_tag="PLP58_09605"
                     /inference="COORDINATES: protein motif:HMM:TIGR00090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0017148 - negative regulation of
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HRJ08813.1"
                     /translation="MESLETARLCAKYADEKKAADLVLLDLRGISPVTDFFVICTATS
                     NPHLRAVRDEVVERMREEHDMKPLGGDGSYESQWLVVNYPNVLVHVLSPEKREFYKLE
                     ELWGDAPRLPWEEGAPAAAEVKPPAKKAAARKAAAKKAPKKAAAKKAAAKKTPAKKAA
                     RRKDG"
     gene            11071..11919
                     /locus_tag="PLP58_09610"
     CDS             11071..11919
                     /locus_tag="PLP58_09610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ08814.1"
                     /translation="MNARNITFALVVLSLLYGIWHYHSDAGRLHAEMDSLKTSHLAEV
                     ERLTREMQSALAAQNDQHQKALQSLNDEHDKKLNELRQSQRTQMAAAYKEFENIFAGN
                     RQTIEYINLLESRVKAGQQLSRIEVEKLTAITSGIGFLQKQYQKPLAEFTALRDYLED
                     AASETAGEKPKSRFGFFKRMFSKDFREAEREQLREEGARRAFAEAQAKFDTVYASAQR
                     SMRAVNLDAEGTLKKLADLIEDKNAANAGDLGAFFDKARQALRTHQSVLDFEPDAVPQ
                     PPKVQP"
     gene            12277..12519
                     /locus_tag="PLP58_09615"
     CDS             12277..12519
                     /locus_tag="PLP58_09615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ08815.1"
                     /translation="MPPAPFKYELPTDFPRSVLREYTIESGFIGVNSRRAMKQIASSK
                     NAPDFGPEHADTFRRDLHPDLRADWQIASVEVRMWN"
     gene            12526..12900
                     /locus_tag="PLP58_09620"
     CDS             12526..12900
                     /locus_tag="PLP58_09620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007417245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four helix bundle protein"
                     /protein_id="HRJ08816.1"
                     /translation="MSEELKKRTKKFALDVIELVAGVPQVLETRHAIGQVIRSSSSVA
                     ANYRSACRGKSKADFIAKLGTVEEEADETGFCLEMLRDIAHRRRLKLETEASQELTRL
                     LDEANQLVAITVASRKTARGGE"
     gene            13172..15331
                     /locus_tag="PLP58_09625"
     CDS             13172..15331
                     /locus_tag="PLP58_09625"
                     /inference="COORDINATES: protein motif:HMM:NF016724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HRJ08817.1"
                     /translation="MNLPRHPKAKHFHGLGLIAGVQSFAELEARIVKLPDEQARGAAL
                     EVFAEACLATQRIYQAREVWPGNSMPSALRQQLRLPMADMGVDGVFVSSSEEPVCYQS
                     KFRTGRPALTWTELSTFYGLADVGARRLVFTNCDEIARVAEERLGAIFVRGSDLDRLT
                     SEDFQIIEAWLAERPATPKKKDPKPHQVVALNDIVAGLSREPRATALMACGSGKTLVA
                     LWAAERLGARTVLVLLPSLALVRQTLHEWLHETGWPDVQFLCVCSDPTVQPEEDSLLV
                     RPSDLDFAVTTQAADVRRFLERPTNSVRLVFSTYQSSQVVKEAVVGLPPFDFGVFDEA
                     HKTAGRDGQKFALALKDENLPITLRLFLTATPRHYDVAKKDKFGESKVVFSMDVPEAY
                     GQVVHRLPFSAAAKAGIITDYKVIISVVTSEMVTNEALRLGVVLVEGDEVKARQVANQ
                     IALKSAIEKYNVSKVFTFHSKVNSAKSFTSGGPEGIGTHLQGFHCAHIEGAMTTAYRE
                     RLLREFAAAPCAILSNARCLTEGVDVPAVDMVAFLSPKRSLVDIVQATGRAMRLSPET
                     GKQFGYVLVPLYVEKARGESIEQAVLRSNFDEVWKVLQGLKEQDDLLAQIIAEMRIER
                     GRIGGFDDSRFRERVELLGPELSLETLRSTITAAYLDAIGEHWFERYGQLVAYKQKHG
                     NCDMPARWDENQKLATWLVDQRVRKRDGTLEPEKSSF"
     gene            15328..16128
                     /locus_tag="PLP58_09630"
     CDS             15328..16128
                     /locus_tag="PLP58_09630"
                     /inference="COORDINATES: protein motif:HMM:NF015422.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helicase associated domain-containing protein"
                     /protein_id="HRJ08818.1"
                     /translation="MNRLSFKWNPRETNWRGVYLELIEFRKRFGHCRVPQNWKENNSL
                     AHWVKTQRMDYAKGELERDRIALLEKIGFEWKVGLGTWDERFAELCAFKQRFGHTRVK
                     VKWAENPLLGAWVVSQRYKHRRKELGEEYVQRLNAIGFDWDGRIAARPVEKAPSDPDK
                     NWREIFERFKAYATANGADVVTVVDEETKKLNRWMLYQRQAKKRGELSEARINALNEI
                     GFLWQRNARRTGARAPRTPVVEVPITRTWDEMLCMSTTRSSSTAPSTQ"
     gene            complement(16327..16539)
                     /locus_tag="PLP58_09635"
     CDS             complement(16327..16539)
                     /locus_tag="PLP58_09635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ08819.1"
                     /translation="MRTTEVRQQIDAMSDEDRFFASAYLQHLANEHDEDRKARLDNRM
                     NRMDSGRKFTLEHLTELHQNLESQGL"
     gene            complement(16595..19495)
                     /gene="ileS"
                     /locus_tag="PLP58_09640"
     CDS             complement(16595..19495)
                     /gene="ileS"
                     /locus_tag="PLP58_09640"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009962343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004822 - isoleucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006428 - isoleucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HRJ08820.1"
                     /translation="MSAEPAPKRNYKDTVRTPKTGFPMKADLVIREPERLARWEASGL
                     YQRIMKQMAGKPKFILHDGPPFANGDVHMGTALNKVLKDLIVKSKTMAGFHAPYIPGW
                     DCHGLPIEFKVVKEAKDLAPAEVRTRSEAFARGFVEVQKKSFKRLGVFGDWDNPYLTL
                     TPAYEADIMRTFAKFVEKGLVYRAKRPVLWSFGAQTALAEAEVEYKEKTSPAIYVPFA
                     INNPPPGLEGSHLVIWTTTPWTLPANLGIALHPNFDYVSGSFTNAEKAHTLKLVIATS
                     RLEAFQKETGYVLDPAHAPEKFKGSLLNGGEAQHPFLPRGSKIINALFVTDDTGTGAV
                     HMAPGHGTDDYIAGREHGLDILSPVDDAGCYTAECGLPDFVGKHVFKSNEGIIELLQS
                     KGALLGNTPYVHQYPHCWRSKTPIIFRAVEQFFIRISDFRADALKAIDTVNWLPAWGR
                     NRIHGTVESRPDWCISRQRTWGVPLPVFYDANGQAIMDAAISHKVADAVEKHGTNLWF
                     EKDDAFWSDLVGLPAGSKRCKDTLDVWIDSGSSSVAVLDRHPELHCPADVYIEGTDQH
                     RGWFQSSLMVSVAVRGAAPYKTVITNGFVVDTSGKKISKSDQGSDKAAKPMTADHYYN
                     KYGGDMVRLWAASVDYQNDVPFSEELFQQTGESYRRIRNTFRVLLGNLHDAPKSTDGA
                     DSADKTSETKSEKSVKSADLTLIDRWILERLHVITSECLAGYAAYDFRKVVSTITQFV
                     SGDLSALYIDITKDRMYCDAENSPRRRATQAAIREITETLVKLLAPILAYTADETWEF
                     LGHTDSVHLQPFPQVGVQASACPGVDSVAQSTPVSPKGFTPTVGVQASACSGTDSTLP
                     AVEDLLKARAVIQQAIEAARQAKQIGSNLEATVKLTLPEKGFTHAIFSDLTALQEFFI
                     LSDLHLTRGPDLAATVEVCTNPKCERCWRLLPDVGTHAEHPTLCGRCAEAVS"
     gene            complement(19536..21068)
                     /gene="dnaB"
                     /locus_tag="PLP58_09645"
     CDS             complement(19536..21068)
                     /gene="dnaB"
                     /locus_tag="PLP58_09645"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HRJ08821.1"
                     /translation="MAEPVSNLIQTAVAPGELPPRAQDKKQANPFGRKRDEEPSAEEL
                     LANINRALPFSDEAEKGLLSSMLQDPNERLADCRVTVPKAAFYHEANRTVYEMLLEFD
                     DKHLPIDPVTVTNALRDRALLDRVGGPAAISDLFTFVPVPSHYPFYKKIVLDKHILRQ
                     LIHACSLNIHYAYEHGKEQADEDVGTLVDRAEQRMLAVRESSGKEDDIKALSAHVAEA
                     IDSIQYMLEHPGQLRGLSTGYSKLDQLSSGLQGSEMFVIAARPSMGKTSLAMNIVEHI
                     AVDCGRPCAVFSLEMSATMLVRRLLVSRAQLSMQDLARGLLSRAQQEALAKATRELQK
                     ASIYIDETPGLDVLEMRAKARRLKKMHGIEMIMIDYLQLMTSSSKRAKDNRQNEIAEI
                     SAGVKGLAKELNVPVIVLAQLNRAVEARKGQRPVLSDLRESGSIEQDADLVGLLTRED
                     YAGAKMAVEDEEAEAKKKGQAVLILAKNRNGPTDDVPLRFIDHAMRFTERAEDEPEPE
                     SP"
     gene            complement(21144..>21665)
                     /locus_tag="PLP58_09650"
     CDS             complement(21144..>21665)
                     /locus_tag="PLP58_09650"
                     /inference="COORDINATES: protein motif:HMM:NF012546.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HRJ08822.1"
                     /translation="GERGTDLELLKKHGPPKLVEAGQKLPAIVASLQCEKARIWDPHG
                     VKAVTDHLAATLRVDTRRIYLTGLSMGGFGTWETALEHPETYAAIAPICGGAGVRWVM
                     AERIKDLPCWIFHGDQDKAVPLEFSTKIHDALIKAGSQAKLTIYPGVGHDSWTQTYEN
                     QEFWDWLLAQKRE"
BASE COUNT         3948 a         6717 c         6858 g         4142 t
ORIGIN      
        1 cgggcgccgt gcagacgcgc gaggcgcgcg gcgccgagac cgctgcggcc ggcgccgaga
       61 atggcgatgt tttgatcctg gagtgacatc aaggcttgga gaaaaggcgg tttgaagttc
      121 aacgcagttt cagggaggcg agtcccatga gggcaaagag gatggagagg atccaaaagc
      181 ggacgatgac ctggttctca tgccagccgc ccagctcgaa atgatggtgg atgggggaca
      241 ttctgaagac gcgcttcccg cgggtcttga agctggccac ctggatcatg acgctcgccg
      301 cctccatgac gaagactccg ccgacaagca cgagcacgat ctcctgcttg cagccaatgg
      361 ccagtgtggc gatgcagccg ccgagcgcta gcgagccggt gtcacccatg aacatgcggg
      421 cggggtgggc gttgaaccaa agaaagccga ggcaggcgcc cgcgagcgac atggcgatga
      481 tgggcagttc attggccagt gagtgatgcg ccacgccgag atattcggag tagttggcgt
      541 tgccgcagac gtagccgaag accatgtagg ccagggcggt cgtcagcgag cagcccgtgg
      601 ccaggccgtc gagtccgtcg gtgaggttca ccgcgttcga agcccccacg atgaccaggg
      661 tgaagagcag cacggcaaag aatcccatgt cggcgattag cggctccttg agaaaaggca
      721 catacaaagc ccggtaggtc tccgtgccgc ccatgtgcag gatgggggcg tcctgccccg
      781 ccttgctggc ctggtccacc agcacgtccg gaggcatgag gtagcaaaag gcaagaccgg
      841 ccgcgaaggc gacaccaccc tgccagacaa gcttggcgcg ggcgctgacg cctttggagt
      901 tcttcttgct gatcttcaga tagtcgtccc aaaaaccgag cgcgccgagg ccgagcgtgg
      961 caaaaaggat gatccaaacc atgaggttgt cccacttggc ccagaccagc gtggagacga
     1021 gaatggcgcc cacgatgaga atgccgccca tggtgggcgt gcccgctttt ttcccgtgca
     1081 actcgtagag cttgttgacc tcctcggcgg tgcgcacggg ctggccgatt ttcagggaga
     1141 tgagcttgcg gatgagccag ccgccgaaaa acacggacag aaaaaacgcc gtgaccgccg
     1201 ccccgccggc gcggaaggtg tggtagcgga aaatgttcag caccttgtaa accagggagt
     1261 ccaccgtgat ccattgcccg gcctcgagcc agtttcgaag ttcgtagagc cagtagatca
     1321 tgattgctga taaagttcga gcacgcgctc catgcgcgtc gagcggctgc ctttgacaag
     1381 cacggcatcc cccggcttca cgagctggcg caggtgcgcg gcgcattcct catgcgtggc
     1441 aaaatgagcg gtgtggcagg ggtcgaaaga ctccgtgtcc gccgccgcct ggctgatcag
     1501 ccgcgcctct ccgtcaccca cggtgatgac ggagtcgagg gtgctggccg cgcttcgacc
     1561 gatgcgcgcg tgctcctcct ccgcgcgcgc tcccagctcg cccatgcgcc cgagcacggc
     1621 gtgtttgcgg cctttgccgg tggtccttag cgccagaatg ccggccatga cagagtctgg
     1681 attggcattg tacgagtcgt tgatgaaaac gactccgccg atgtcgagca cctgcatgcg
     1741 cccgccggtg atgcacgccc tggaaagcgc ggcggcgatg tcctccgggg cgatgccctg
     1801 cgcccagccc atgcaggcgg ccagcgccgc gttcaccacc atgtggcggc cgatgacggg
     1861 gaggaaaact tccgcgcttt ccccgcggaa atcgagtttg aagcgcgtgc cgttttccac
     1921 ccgctcaagt tccgaggcgc tgacatcacc ggactcgatg ccggcggtga gcacccgcgc
     1981 ctggcaatgc cgcgcgatga gcggcgtgta gtcgtcatcc gcgggcagca cgacggtgcc
     2041 gccgccgggc acgttcgcgg gcagggtgcc tttttcccac gcgatggcat cgcggctgcc
     2101 catgtgctcg atgtgggcga tgcccacgtt ggtcaggatg gcggcatccg gcagggctgt
     2161 gtccaccagt gtcctgatct cgccgggatg gttcatgccc atctcgacga cggcgcactc
     2221 atcgccatcc tggagcgaca gaagagtcag cggcacgccg atgtggttgt tcaggttccc
     2281 cgccgtggcg cgggtctgga acttcgccga catgacggcg gcggcgaagt ccttggtcgt
     2341 ggtcttgcca ttgctgccag ttaggccgat gaggagcggc ttgtgccatg agcggtagcc
     2401 gcgcgccaaa ttttgcagcg ccgtcagcgt gtccggcgcc tccatcagcg cgcagtgctc
     2461 cggcaggctc catttttcat ccacatggct cacgatcaca gccgaggcgc ctgacgcagc
     2521 cgcttgcggg atgaaatcat gcgcgtcaaa cctctccccc ttcaaagcca cgaacacgtc
     2581 cccaggctga accgcgcgcg agtcagtgac cacccgcgtc accgcgcgcg tagaatcccc
     2641 cgccctgcgg acgatgccgg ggcagaagga aagcagcgtt tggagtggaa tggctttcat
     2701 catttaatca cgccaagggc ctcgcatgtc ctggtccctc atttcctgct ctcgcagggc
     2761 ctcgcgctcg gcggcttccg cgtcgcgctc gtggaagagg tcgttgagtg tctgccgcac
     2821 cacgcggcgg tcgtcgaagg ggtgtttttt gccctggatg tcctggtagt cctcgtgccc
     2881 tttgccggcc acaagcacga tgtccccgcc ctgcgcgtgc tccaaggccg cgcggatggc
     2941 ctggcggcgg tccgcgatgg cggcatggct tttcgggcgc gcgaagcctt tgatcgtgtc
     3001 ggcaatgatg gcctcgggat cctcaaaacg cgggttgtcc gaggttagga cacaaacatc
     3061 gctgccggcc tcggcggcgg ccgccatctt cgggcgcttg gtgcggtcgc ggtcgccgcc
     3121 gcagccgaag acggtgatga tgcggcgcgg ccggagggcg cgcagagtgc gcaaggcgtt
     3181 ctcgatggcg tccggcgtgt gcgcgtagtc cacatagacg tggaagcgcg tctggtcggt
     3241 gacgcgctca agacggcccg ggacctgcgg ggcgcttttc agatgagcga cgctttcgcg
     3301 caggttgagg cccatggcat gggcgccggc cagcgcggcc agggagttat agacgttgaa
     3361 gtcgccgatc agaggaaggc gcaccagcaa gtgccgtccc agcgcctcca actcataggt
     3421 ggtgccggtc acatcatagc gcacattggt ggcgcggaat tccagccccg cgccgaagcc
     3481 gtaacgaacg accttgccgg tgccctcgaa gcgggtgatg agcttgcgcc cccaggcatc
     3541 gtcgccattg acgaccatgc ggcctgtttt ttgcgcggcg attttttcaa acaagcgcgc
     3601 cttggccgcg aagtatttct ccatcgtgcc gtggtagtcg agatggtcct gggtgaggtt
     3661 ggtgaaaatg ccggcggcaa aatccagccc gtggacgcgc tcctggtcca gggcatgcga
     3721 gctgacctcc atgacgcagg tgcggcagcc attgtcgcgc atttcagaga gcagtccttg
     3781 aatctccagg gactcgggcg tggtgtgggt ggccggcttc ggcttgcgcg cgccagtgtc
     3841 ccaggagacg gtgccgatga gaccggcgcg cttctgcccc tggttgagca ggtcgtgcaa
     3901 aagaaaggct gtggtagtct tgccattcgt gcccgtgatg ccggcgagcg ccagcacacg
     3961 ccccggatca tcatgcaggg cggcggcggc ggcggccagt gcgcggcgca tggagcgcgc
     4021 atgaacccag ggaatgaccg catccgcagg cggcgcggtc tcggcaaaaa tggcggcggc
     4081 tcccgcagtc atggcggcct gaatgaaatc gtggccgtcc gcctgcgctc cgcgcagcgc
     4141 aaagaaggcc gagcccggac cggccttgcg tgaatcatag gccagcgcgc ggatgtccgt
     4201 gtcaaggctg ccggacacaa cgggctgctc cagccgggcg acgatgtccc tcaacttcat
     4261 tgaccgcctc ctttcaaggc cagcggacgc tggggtctga cgctgagttg tttcaaggtt
     4321 tcctcgacga tgcgcgcgaa gacaggcccg gccaccgcgc ctccgccgat gacgtcggtg
     4381 tccagcgact ttttcggatt gtccaacacc acgacacaag cgatttttgg atcattggcc
     4441 ggcgcgaagc cggcaaagga gacaacccac tggtctttgc gatagccgcc ttttttgggc
     4501 gtgccgtcct tcctgtagcc tgtgaccatt tcggggtcaa aacgctgcgc ggtgcctgtc
     4561 ttgccagcca cttcaatgcc ttcgatctcc gcggctttgc cggtgccttt ggcgacgacg
     4621 tttttgagca gttcgcgcat catctgcgcg gtgcgcccgg agcagacttg gcccgccact
     4681 tccggctgca aatgctcgac tcccctctca ctggtcacgc gatccacgag gcgcggcttc
     4741 atgagaagtc cgttgtttgc cagcgcgccc atggccagcg ccatttgcag gggagtcacc
     4801 tccacctcgt aacccatggg aaaactggag taggtcgtgt tcgaccagtt gctccagttt
     4861 atcttgcccg cgctttcacc gggaatgccg cagccagtgg cgctgccaaa gccaaagcgc
     4921 cgtgtgtagt cgagaaacac agcctggcct gttttcttga agattttgta ggtgccgatg
     4981 ttgctcgaat gggtgaacac atcacgcgct ggcacggtgc ccattttggt caagtcttcg
     5041 aggtcgaaat tgagaaccgg gtcgtgatac ttgccccagt ggcagtcgaa acgctcgtcc
     5101 accgtgatct tgcgctggtc aagagctgcg gtgaaagcca cgatcttgta agtggaaccg
     5161 ggttcgtagg gtttgttcac ggcgaagtgc tcccagtttg tggcttcaga ctggccggcc
     5221 agcgcgcggt cgtgggcggg cagggagctc atcgccagca ccgtgccggt ggtgacctcg
     5281 gtgaccacgg ccatgctgcg ggtggctttc cagcggttgt gcgcctcgat caggatcttg
     5341 tccacggcct cctgcaaagc caggtccaga gtcaggtgga ggccgctgcc gtggcgcggg
     5401 tctatcatct cgccgcggta aagcagcatc tcctccccgc gcttgttccg ctcgatctgc
     5461 acgctgccat tctcgccatg aagaatggac tcgcggccct tttcaatgcc ataggtgccg
     5521 cccttgcccg gcgcggtgcc gccaacgatc aacgccatgc tgttcccggc ggggtagctg
     5581 cgcttcaccg cgggcttcac atacaccccc ttgatctgca tggcttcgag atacccggcc
     5641 cactcagcgg atttttcgtc gtccatttca cggaccagtg tctgcacagg ctccttggac
     5701 ttgaccacgg ccagcatctc cggcacctgc cggcccagct tggcggccag cgccctggag
     5761 acatggtcgt ggtaagcgtc aaagatggcg cggtcgctca tcttgagggt caattcacgg
     5821 accgtcatgc ccttcacagc agccaggcag ggcttgatgg tgggcagctc gtgcagatgc
     5881 acctggttgg tgcgcagctc atggacctgc tcgtcataag caaggagcct gccgctgaag
     5941 tccttgatgg tgccgcgccg cgccagcagc acgcgctcct cgcgaaactt ggccgcggcc
     6001 agctccgcca gacgcggcgc ctggcgcaca tgcaggtcat aaagtcgcca caccacggcg
     6061 gaaaaacccg tcacgagaag gctggcggcg aggatcatgc ggcgaagctc ggctgcatgg
     6121 cggcatgggc gggctggttt ggcggctggg ttcactgggg agagctgttt tgagccaccg
     6181 gaatggaggc ggatttcagg taaatgacgg atctggcgtc cttgttgatt tcctggagct
     6241 gcagggcttt gtccgccagc acgcccgaga cccgcttttt gtcgagaagc tgggtgccga
     6301 tcttgttgtc caggttggtg atctcaaact tcagcagacg ggtctcctgc tccaccaggc
     6361 gctgctggtc cgccagcgcg gtgatgcggt ttttgttcac caccacgctg agcgccgtca
     6421 gcagcagcag cacaatgatg gtgagcagcg ccgagaggcg cggcaggctg aggccggagc
     6481 tgttgcggcg gtttttcatg cggggagtct ctctgcggcg cgcaggcggg cgcttcgcgc
     6541 gcgcgggttc agcgtcactt ccgcctccgc cgcctcgaca ggcttgcgcg tcagcactcg
     6601 cagcaggcag tccgggttcg gacgcggact ggcccactct tttttgtcga ggaatggcgc
     6661 gctcatccgc tggaatgcct gcttggtttc ccggtcctct gtcgagtgga acgaaatgac
     6721 caccagccgc cctccgggtt tcagccagcg cggagcctcc tgcaaaaact cccgcaacgc
     6781 ggcgatctcc tggttcacct cgatgcgcag cgcctgaaag gtcagtgttg cgggatgttt
     6841 gcgcccgcgc cccggacaag cgcgctcaat caattccgcc agctgcaggg tgctggtgaa
     6901 gggctgcctc gcgcgctcgc gcacgatcgc ccgcgcgatg cgcatggcat gaggctctcc
     6961 gccgaattcg cggatcacgc gcgccagatt ctccaactca taaccgttga ccacatgcgc
     7021 cgcagtcaat gaggcgccgc gatccatgcg catgtccagc gggccgtcct tctgaaacga
     7081 aaaccctctt tccgccgagt cgagctgcct gctgctcaca cctatgtcaa ccaggatgcc
     7141 atccagcccg ctgactcccg cctcgcccag caccttggga aagtcgcgaa agttcccgcg
     7201 cagcgcgcaa aacctgtcct cataacaagc cagcctgcgt ttcacatgat ccagcgcctc
     7261 ctcgtcctga tccattccga ccacccgggc gccatgctgg agcagcgcct ccgcgtgacc
     7321 tccccctccg actgtgccat caagaaatag cttgccggag cgtggctgca aaaagtccag
     7381 cacctgctgc gtcaagaccg gcaaatgata aaagggcgtc tgtctgccgc cttccgaatc
     7441 gtgcggaggc tgtgattcag gctcatgatc cccaccttgc ggacgcgaca cacactttcc
     7501 cccgccccag ggcatctgtt catccatggc ctccgaagct ccaaaccatg gccacacccc
     7561 cacctggaaa aattcaaaaa aacgcggttg caacgccgag ctgcgctgcc cgttcccgcg
     7621 caggatttct tttttctgga aaatactcca aaaattggaa aaaatgttta gcgttgacga
     7681 tgtcatggca gatcttcggg aaagcagcct ggaagccaag gtcagcgttg ataattaatc
     7741 tctgcgacct attttcgtca tcttaatatt cggaaatcca agagatgtaa atagtgcatt
     7801 tcgctttttt tggaaaatct ccaatattgt gaataaaatg attttaagtt gtgttaatga
     7861 acagtaattt gagatttctt tcctcgactg ggtttggggt tagtctcgca ggctcatgat
     7921 tcagtctcac ctccccgaac tcctctcccc cgccggcaac tgggactgcg cccgcgccgc
     7981 cgtggccaac ggggccgatg ccatttattt cggcctgccg cgcttcaatg cgcgcatgag
     8041 ggcggacaac ttcactgcgg aagacctgcc ggaactcatg cgctttctgc atcggcacgg
     8101 agtcaaaggt tactgcgcct tcaacgtgct catcttcacc cgcgaactgc cggacgcggc
     8161 aggacagctt caactcctcg aacaatccgg cgtggatgca gtcatcgtac aggacatcgg
     8221 cctcgcgcgc ctggtcaagg ccctcactcc gcgcctgcgc cttcacgcca gcacacaaat
     8281 gaccctcacc tcgccggagg ggctcgaact ggctcgcacg ctcgacattg accaggccgt
     8341 gctcgcgcgc gaactaagcc tgcgcgagct ggagcgcttc cacgcggaag aaaatccggc
     8401 gctgccgctg gaagtcttcg tccacggcgc gctctgcgtg gcctacagcg gccagtgcct
     8461 caccagcgag tccctcggcc agcgcagcgc caaccgcggc gagtgcgcgc aggcctgccg
     8521 catgccctat gagatgctcg tggatggcga gccgcgcgat ctcggcgaca agcgctacct
     8581 cctcagcccg caggacctcg ccgccgtgag ggaaatcccg cgcctcatcg aactcggcat
     8641 ccgcagcttc aagatcgaag gccggctcaa gacccccgaa tacgtcgccg ccgtcacccg
     8701 cgtgtatcgc aaggccattg actccgcccg cgccgctccc ggcgcgcccg ccgcattcac
     8761 cgaggatgac cgctacgagc tggagatgac cttctcacgc ggcctctact ccggctggat
     8821 gcacggcgtc aaccaccagg agctcgtcgg cgccatctac ggcaaaaaac gcggcatgcc
     8881 cgccggtgtc gtgcgctcca ccgcccgcga cgccgtggaa ctcgaaagcg tccccgccca
     8941 tctgaaaacc ggggacggac tcgccttcga gaaccttcac gacaccaacg acgagcaggg
     9001 cggccgcgtc tatggcatcc gcggacgctg gatcgagttc cagcgcggcc ggctcgacac
     9061 ctcccgcatc ccccccggca cgcgcgtctt caaaaccggc gaccaggtgc tcgaacaacg
     9121 cctgcgccag acctggcagg gggagattcc acagcgccgc aagacccggc tcgatctcgc
     9181 cgtcagcggc aaagcgggcg cgcccctcct gctcgaatgc gacgcgccgc gtgcccgtgt
     9241 ggagtccgcc accgctcttc aacccgcgga gcgcagtccg ctgacacgcg agattttgca
     9301 aaaccagctg ggccgtctgg gcgccaccac ctatgaactc ggatctctgg acatgcgcgt
     9361 cgaaggcgcg gtcatgctcc cggtcagcga gctgaaccgc atgcgccgcg ccctggtggg
     9421 gcaagtctcg caaaacagcg aggctccagc agccgcgccc tcctccgctc cccgttccag
     9481 ccttgatgaa atgctcgcag ccgtcagcgc catgcgcgcg cccgccggcg atgagccgcc
     9541 gcgcctgcat gtgctctgcc gcacctttga gcatgtccag gccgcgctcg acagcggcgt
     9601 cacgcgcctc tacgccgact tcgaggacat ccgcctctat ggcgatgtgg tggggcgcgt
     9661 gcgcgcgcag ggcggcgcct ccgtgtttct cgccacgccg cgcattcaga aatccggcga
     9721 ggccggcttc ttcaaactca tcgcccgcgc gcagccctgc ggcgtgctca tccgcaacct
     9781 cggcggcatc gcctttttca aagacgccgg actgcccgcc atcggagatt tttccctcaa
     9841 taccgccaac ccgctcaccg ccggtttcct caaagacacc ggacttgggc gcctcaccat
     9901 cagctacgat ctcaccatcg agcaagtgct cgacctgctg cgctccgcgc cgccgcagtg
     9961 gttcgagatc accctgcacc agcacatgcc catgtttcac atggagcact gcgccttcgc
    10021 cgcctttctc tccgagggca cggacttcac caactgcggc cgcccgtgcg agaggcaccg
    10081 cgtccacctg cgcgaccgcg tcggcatgga gcacccgctc aaggccgacg tcggctgccg
    10141 caacaccctc ttcaacgccg tgccgcagac cggcgcgcag ttcttcgcgg acctcatggc
    10201 cgcgggcctg cgcgactacc gggtggaact gctggaggag gacgccgcgg aagcgcgccg
    10261 cgtcatatcg gcctaccagg atttgctttc cggcagcagc ggcggcgaca tctggcgcgc
    10321 gctcaaggcg cgctcccaga tcggagtcac gcgcggcacc atggccggac gctcattcta
    10381 aaaaaccgtt cagccaggca ggttacccat ccttccgccg cgccgccttt ttcgcgggcg
    10441 tcttctttgc ggccgctttt tttgcggccg ctttttttgg cgcttttttc gcggcggctt
    10501 tcctggccgc agccttcttc gcgggcggct tcacttcggc ggcggcgggc gcgccctcct
    10561 cccatggcag gcgcggagcg tcaccccaga gttcctccag cttgtaaaac tcccgcttct
    10621 ccgggctgag cacatgcacc agcacattcg ggtagttcac caccagccat tggctttcat
    10681 acgagccgtc gcctcccagc ggcttcatgt cgtgctcctc gcgcatcctt tcgaccacct
    10741 cgtcgcgcac ggcgcgcaaa tgcggattcg acgtggccgt gcagatgacg aaaaagtccg
    10801 tcaccggcga gatgcctcgc aggtcgagca ggacgaggtc ggccgctttt ttttcatcgg
    10861 cgtatttggc gcacaagcgc gcggtttcga gggattccat gagcggggtt gaggcgcgca
    10921 tcaaaacgcc ggattgcccg gaaagcaagc cgccatcggg accgcaccgt tcccgccgca
    10981 aaaaacgcgg agcaaaaacc ccgcagtttc cgggaatgaa ccttgcgcat tccggccgcg
    11041 cgcgcaggag tacccgcgaa cccccttccc atgaacgcgc gcaacatcac cttcgccctc
    11101 gtcgtcctct ccctgctcta tggcatctgg cattaccact ccgacgccgg ccgcctgcac
    11161 gccgagatgg actcgctcaa gacctcccac ctcgcggaag tcgagagact cacccgggag
    11221 atgcagagcg ccctggccgc gcaaaacgac cagcatcaga aagcgctcca gtccctcaac
    11281 gacgagcatg acaaaaaact caacgagctg cgccagagcc agcgcaccca gatggccgcc
    11341 gcttacaagg agtttgagaa catcttcgcc ggcaacaggc agaccatcga atacatcaac
    11401 ctcctcgaaa gccgcgtgaa ggccggccag cagctctcca ggatcgaggt cgaaaaactc
    11461 accgccatca ccagcggcat cggcttcctc cagaagcagt atcaaaagcc cctggccgag
    11521 ttcaccgccc tgcgcgacta cctcgaggac gccgcctccg agaccgccgg cgagaagccg
    11581 aagtcccgct tcggattctt caagcgcatg ttcagcaagg acttccgcga agcggaaagg
    11641 gagcagttgc gcgaggaggg cgcgcgccgc gccttcgccg aggcgcaggc caagttcgac
    11701 accgtctatg cctccgccca gcgctccatg cgcgccgtca acctcgacgc cgagggcacg
    11761 ctgaaaaagc tcgccgacct aattgaggac aaaaacgccg ccaacgccgg ggatctcggc
    11821 gccttctttg acaaagcccg ccaggctctg cgcacccacc agagcgtgct cgacttcgaa
    11881 cccgacgccg tgcctcagcc accgaaggtg cagccgtagg atggcgggcg cgcctgccag
    11941 cgcctttcgg tttgcccatg agcggggtcc tctgttatgc agggggcgca ttcacccgcc
    12001 gccctgactt gagccatccc gacacagaag acgcgccagc gtccccggat caccgaacac
    12061 cccggcaagg acaccgccat caccgccccc ggatcacccg ccatcccggc taggatcgag
    12121 ccataaccca ccccagaatc gccaaatccg gaccaaaacc accgaaaaca ccccaactcc
    12181 aagtctccaa gtctcctctt ctcacggtct caaagtctcc cgtccccctg tcttgccatt
    12241 cgccgcactc cctgcttgga tgaggcgaat tccctcatgc cgcccgcccc gtttaaatac
    12301 gaactgccca ccgacttccc gcgctcggtc ctccgggagt acaccatcga atccggcttc
    12361 atcggcgtga actcgcgccg ggccatgaag cagatcgcct catccaagaa cgcccccgac
    12421 ttcggccccg agcacgccga caccttccgc cgcgacctcc atcccgacct ccgcgccgac
    12481 tggcaaattg cgagtgtcga agtgcgaatg tggaactgaa aagccatgag cgaagaactg
    12541 aagaaacgaa cgaagaagtt tgccctcgac gtcatcgaat tggtggctgg cgtgcctcag
    12601 gtgttggaaa cccgccacgc catcgggcaa gtcattcgtt cgtcgagttc ggtcgctgcc
    12661 aactaccgtt cagcgtgtcg tggaaaatcg aaagccgact tcatcgccaa actcggaacc
    12721 gttgaggagg aagcggacga aaccggcttc tgtctcgaaa tgctccgaga catagctcac
    12781 cgccgacggc tgaaactcga aaccgaagca tcgcaggaat tgacgcgttt gctcgacgag
    12841 gcgaaccaac tcgtggctat caccgtcgca tcacgcaaaa cagcaagggg aggcgaatga
    12901 tgaatttcga acttcaaatg acgaaccggg aaatgcagaa tggcgaagtg cgaacgtcga
    12961 accgcgtctc gttcagttcg acattcgaaa ttcagctttc ggcattcgcc ccgtccctcc
    13021 caagggcaac gccaacttcg cctgctgctc cattcgccat tcgaaattcg gacttcagca
    13081 ttcgcctcgc ctcgccccgc agggcatggc cggcttcgtc ctcgccaatg gcagcatgaa
    13141 acatgtgagc aattcattct gaaagacggc gatgaacctg ccgcgtcatc ccaaagcgaa
    13201 acattttcat ggcctcggct taattgcagg ggttcaaagt ttcgccgagc ttgaagctcg
    13261 cattgtgaaa ctgcctgacg aacaagcgag aggcgcagca ctcgaagtct tcgccgaagc
    13321 gtgtttggcg acgcagcgaa tttatcaggc tcgcgaggtt tggccgggaa actcaatgcc
    13381 ctcagcactg cgccagcaac tccggttgcc aatggcagac atgggagtgg atggagtatt
    13441 cgtcagttcg tctgaggaac ctgtctgcta tcagtcgaag ttccgcactg gacggccagc
    13501 gctcacttgg accgaactgt cgacttttta tggcttggct gatgtaggcg cgcgcaggct
    13561 cgtctttacc aactgcgatg agattgcccg cgtcgcggag gaacggttgg gagccatctt
    13621 cgtcagagga agcgacctcg accggctcac gtctgaggat ttccagatca tcgaagcgtg
    13681 gctcgcggag cgacccgcga cgccgaagaa gaaggaccca aagccgcacc aagtcgtcgc
    13741 gttgaacgac atcgtggccg gactgtcgcg cgaaccacgg gccacggcgt tgatggcttg
    13801 cggcagcggc aagaccttgg ttgcgctatg ggccgctgaa cgactcggcg cgcgcaccgt
    13861 gctcgtgctg ctgccttcgc tggcgcttgt tcgccagacg ttgcacgagt ggcttcacga
    13921 aacgggttgg ccggacgtgc agttcttgtg cgtctgttcc gacccgacag ttcagccgga
    13981 ggaagattcg ctgttggttc gtccgtccga cctcgacttc gctgtcacga cgcaggctgc
    14041 cgacgtgcgc cgctttcttg agcgcccgac caactcggtg cggttggtat tctccacgta
    14101 tcaatccagc caagtcgtca aggaggccgt cgttggcttg ccgcctttcg acttcggcgt
    14161 cttcgatgaa gcacacaaaa ctgccggacg agacgggcaa aagttcgccc tcgcgctcaa
    14221 ggacgaaaac ctgcccatta ctctccggct tttcctgacc gcgacgccgc gccactacga
    14281 cgtggcgaag aaggacaagt tcggcgaatc aaaagtcgtc ttctcgatgg acgtgccgga
    14341 agcctatggg caggtcgttc atcggctgcc attcagcgcg gcggcgaagg ccggcatcat
    14401 caccgactac aaggtcatca tctcggtcgt gacatcggag atggtcacca acgaagcgct
    14461 gcggctcggc gttgtcttgg ttgagggcga cgaagtgaag gcccggcagg tcgcgaacca
    14521 gattgcgctc aagtcagcca tcgagaaata caacgtctca aaggtgttca ccttccattc
    14581 caaggtgaac tccgcgaagt cgttcacctc cggcgggccg gaaggcatcg gcacgcatct
    14641 ccaagggttt cattgcgccc acatcgaagg cgcgatgact accgcctacc gtgaacggct
    14701 cttgcgcgag ttcgccgccg ccccgtgtgc cattctctcc aacgcgcgtt gcctcacgga
    14761 aggtgtggat gttcctgccg tggacatggt ggcgttcctc agcccgaagc gcagccttgt
    14821 ggacatcgtg caggcgacgg gccgggcgat gcgcctatcg cccgaaacgg gaaagcaatt
    14881 cggctacgtg ctcgtgccgc tttacgtcga gaaggcacgg ggcgagtcga ttgagcaagc
    14941 agttctgcgc agcaacttcg atgaggtctg gaaagtcctc caaggtctga aagagcagga
    15001 cgacttgctg gcgcaaatca tcgccgagat gcggattgag cgcgggcgca tcggtggctt
    15061 tgacgacagc cgatttcgcg agcgcgttga actgctcggg cctgagcttt cgctcgaaac
    15121 tttgcgcagc accattactg cggcgtacct cgacgcgatt ggcgaacatt ggttcgagcg
    15181 ctatggtcaa ctcgtcgcct acaagcagaa gcacgggaat tgcgatatgc ccgcgcgctg
    15241 ggatgaaaac caaaagctgg cgacttggct tgtggaccaa cgcgtccgaa agcgtgatgg
    15301 cacccttgag ccagaaaaat cgagcttctg aaccgcctca gcttcaagtg gaatccacgc
    15361 gagaccaact ggcggggcgt ctatctggaa ttgattgagt tccgaaagcg ttttggtcac
    15421 tgccgggtgc cgcaaaactg gaaggagaac aattcgctcg cgcactgggt gaagacgcag
    15481 cggatggatt acgcaaaggg agaactcgaa cgtgatcgta tcgcgttgct ggagaagatt
    15541 ggatttgaat ggaaagtcgg tcttggaacg tgggacgagc gatttgctga actgtgtgcc
    15601 ttcaaacagc gatttggtca cacccgcgtg aaagtaaagt gggcggagaa cccgcttctt
    15661 ggtgcgtggg tagtcagcca gcgatacaag caccggagaa aagagctggg tgaagaatac
    15721 gttcagcggc tgaacgccat cggctttgat tgggacgggc gaatcgccgc taggccagtt
    15781 gagaaggctc cctcagaccc ggacaagaat tggcgcgaga ttttcgagcg gttcaaagct
    15841 tacgccaccg cgaacggcgc ggacgtagtt acggtggtgg acgaggagac gaagaagctc
    15901 aatcgctgga tgctttatca gcggcaagca aagaagcggg gcgagttgag cgaggcccga
    15961 atcaatgctc tcaacgaaat cggattcctc tggcagcgca acgcgcggcg caccggcgcg
    16021 cgcgcaccgc gcactcccgt agttgaagtg ccaattaccc gcacttggga cgagatgttg
    16081 tgtatgtcca caactcgaag ttcatcaact gcgccatcta cccagtaggt gatgagaaag
    16141 ggatggaggg ctttgttgct gagccagcgg cctgatcggt cttgctcgcg gaagtcgggt
    16201 ggttcgtggg catggtctcg gaggtgctcc agatgggcca tgagtttttt gcgagtggct
    16261 gttggcgagg cgagcacgag acgcatcacg tcttcatcaa gcacgatctg tggcttgtcg
    16321 gccatgttac agcccttgcg actccaagtt ttgatgaagt tcggtcagat gttccagagt
    16381 gaacttgcgg cctgaatcca tacggttcat ccggttgtcc aaacgagctt tgcgatcttc
    16441 atcgtgctcg ttggcaagat gttggaggta agcagaagcg aagaagcggt cttcatcgct
    16501 catggcatca atctgctggc ggacttctgt cgttctcatg cgagtataat gctaggctag
    16561 gaaggattgg caatgctcaa ttgattgccc acgattatga gacggcctcc gcgcagcggc
    16621 cgcagagcgt tgggtgctcc gcatgcgttc cgacatccgg cagcaggcgc cagcagcgtt
    16681 cgcacttggg gttggtacag acttcgacgg tggcggcgag gtcggggccg cgggtgaggt
    16741 ggaggtcgct gaggatgaag aattcctgga gggcggtgag gtcgctgaag atggcgtggg
    16801 tgaagccttt ctcgggaagg gtgagcttca cggtggcttc gaggttggag ccgatctgct
    16861 tggcctgacg cgcggcctcg atggcctgct ggatgacggc gcgggctttg aggaggtctt
    16921 caacggcggg gagggtggag tctgttcctg aacaagctga agcttgaact ccaacggttg
    16981 gagtgaagcc tttaggcgac acgggagtgg actgcgctac ggagtcaact cccggacaag
    17041 ctgaagcttg aactccaact tgcgggaacg gctggaggtg gacgctgtcg gtgtggccga
    17101 ggaattccca ggtctcgtcg gcggtgtagg cgaggatggg ggcgaggagt tttaccaatg
    17161 tctcggtgat ctcgcggatg gcggcctgcg tggcgcggcg gcgggggctg ttctcggcgt
    17221 cgcagtacat ccggtccttg gtgatgtcga tgtagagcgc ggagagatcg ccggagacga
    17281 actgggtgat ggtggagacg accttgcgga agtcgtaggc ggcatatccg gcgaggcact
    17341 cgctggtgat gacgtgaagg cgctcgagga tccagcggtc aatcagagtg aggtccgcag
    17401 atttcacaga tttctcagat tttgtttctg aagtcttgtc tgcggaatct gcgccatctg
    17461 tggatttggg agcatcatgc agattgccga ggaggacgcg gaaggtgttg cggatgcggc
    17521 ggtaggattc gccggtttgc tggaagagtt cttcgctgaa ggggacgtcg ttttggtagt
    17581 ccacactggc ggcccagagg cggaccatgt cgccgccgta tttgttgtag tagtggtcgg
    17641 cagtcatggg cttggcggcc ttgtcgctgc cctggtcgct cttgctgatc tttttgccgc
    17701 tggtatcgac gacgaagccg ttggtgatga cggtcttata cggcgcggca ccgcgcacgg
    17761 cgacgctgac catgagactg ctctggaacc agccgcgatg ctggtcggtg ccctcgatat
    17821 agacatcggc ggggcagtgc agctcgggat gacggtccaa aacggcgacg gaggacgagc
    17881 cggagtcaat ccacacatcg agcgtgtctt tgcagcgctt cgagccggcg ggcaggccca
    17941 cgaggtcgct ccagaaggcg tcgtcttttt caaaccagag gttcgtgccg tgcttctcga
    18001 ccgcatcggc gaccttgtgc gagatggcgg cgtccatgat ggcctggccg ttggcatcgt
    18061 agaaaacggg cagcggcacg ccccaggtgc gctggcggct gatgcaccag tccgggcggc
    18121 tctcgacggt gccgtggatg cgattgcggc cccaggcggg cagccagttc acggtgtcga
    18181 tagccttcaa ggcatcggcg cggaagtcgc tgatgcggat gaagaactgc tcgacggcgc
    18241 ggaagatgat gggcgtcttc gagcgccagc agtgcggata ctggtgaacg taaggcgtgt
    18301 tgccgagcag cgcgcctttg ctttgcagca gctcgatgat gccttcgttg ctcttgaaga
    18361 catgcttgcc gacgaaatca ggcaatccgc actcggcggt gtagcagccg gcgtcatcga
    18421 cgggcgagag gatgtcgagg ccgtgctcgc gtccggcgat gtagtcgtcc gtgccgtggc
    18481 caggagccat gtgcaccgcg ccggtgccgg tgtcgtcggt gacgaagaga gcattgatga
    18541 tctttgagcc gcgcgggagg aagggatgct gcgcctcgcc gccattgagc aggctgcctt
    18601 tgaacttctc cggcgcgtgc gcgggatcga gcacgtaacc cgtttccttc tggaaggcct
    18661 cgagacgcga ggtggcgatg acgagcttca aggtgtgcgc tttctcggcg ttcgtgaagc
    18721 tgccgctgac gtagtcgaaa tttgggtgca gggcgatgcc gaggttcgcc ggcagcgtcc
    18781 acggcgtggt ggtccagatg acgaggtggg agccttcgag gccgggcggc gggttgttga
    18841 tggcaaacgg cacgtagatg gccggcgagg tcttctcttt gtattccact tcggcttcgg
    18901 cgagcgcggt ttgtgcaccg aaactccaca gcacggggcg cttggcacga tagacgaggc
    18961 ctttctccac aaacttcgcg aaggtgcgca tgatgtcggc ttcgtaggcg ggcgtgagcg
    19021 tgagatacgg attgtcccag tcgccgaaga cgccgaggcg cttgaaggac tttttctgca
    19081 cctcgacaaa gccacgcgca aaggcctcgg agcgcgtgcg cacctccgcc ggggcgaggt
    19141 ctttggcttc tttgaccact ttgaactcga tgggcagccc gtggcagtcc cagccgggga
    19201 tgtagggcgc gtgaaagccg gccatcgtct tcgatttcac gatgaggtcc ttgagcacct
    19261 tgttcagcgc ggtgcccatg tgcacatcgc cattcgcgaa gggcgggccg tcgtgcagga
    19321 tgaacttcgg cttgcctgcc atctgcttca tgatgcgctg gtaaaggccg gatgcctccc
    19381 acctggccag ccgctccggc tcccggatga cgagatcggc cttcatgggg aagccggtct
    19441 tcggggtgcg gacggtgtct ttgtaattgc gtttcggggc aggctcggcg ctcatgggaa
    19501 atggagcgcg gagggtatag agaggggggc gggggtcaag gactctccgg ctccggctcg
    19561 tcctcggcgc gctcggtgaa gcgcatggcg tggtcaatga agcgcagggg cacatcgtcg
    19621 gtgggaccat tgcggttttt ggcgaggatg agcacggcct ggcctttctt tttggcctcg
    19681 gcctcctcgt cctcgacggc cattttggcg ccggcgtagt cctcgcgggt gaggaggccg
    19741 acaaggtcgg cgtcctgctc gatggagccg gactcgcgca ggtcggagag cacggggcgc
    19801 tggcctttgc gggcttcgac ggcgcggttg agctgggcca ggacgatgac ggggacgttc
    19861 agttccttgg ccaggccctt cacgccggcg gagatttcgg cgatctcgtt ctggcggttg
    19921 tccttggcgc gcttgctgga ggaggtcatg agctggaggt agtcaatcat gatcatctca
    19981 atgccgtgca tcttcttgag gcggcgggcc ttggcgcgca tttccagcac gtcgaggccg
    20041 ggggtttcat caatgtagat gctggccttt tgcagctcgc gggtggcctt ggccagggcc
    20101 tcctgctggg cgcgggagag caggccgcgg gcgaggtcct gcatggagag ctgggcgcgc
    20161 gagacgagca ggcggcgcac gagcatggtg gcgctcattt ccaggctgaa gacggcgcag
    20221 ggcctgccgc agtccacggc gatgtgctcg acgatgttca tggcgaggga ggtcttgccc
    20281 atggaggggc gcgcggcgat gacgaacatt tccgacccct gcaggccgct gctgagctgg
    20341 tcgagcttgc tgtagccggt ggaaagcccg cggagctggc cggggtgctc cagcatgtac
    20401 tggatgctgt ctatggcctc ggccacatgc gcgctgagcg ccttgatgtc gtcctccttg
    20461 ccggatgact cgcgcacggc cagcatgcgc tgctcggcgc ggtccacgag cgtgcccacg
    20521 tcctcgtcgg cctgctcctt gccgtgctcg taggcgtagt ggatgttcag cgagcaggca
    20581 tggatgagct ggcggaggat gtgcttgtcg aggacgatct ttttgtagaa cgggtaatgc
    20641 gacggcaccg gcacaaaggt gaacaggtcc gagatggccg ccgggccgcc cacgcggtcg
    20701 agcagggcgc ggtcgcgcag ggcgttggtg actgtgaccg ggtcaatggg cagatgcttg
    20761 tcgtcgaact ccaggagcat ctcatagacg gtgcggttgg cctcgtggta aaaggccgcc
    20821 ttcggcactg tcacgcggca gtcggccagg cgctcgttgg ggtcctggag catgctggag
    20881 agcaggccct tttccgcctc gtcgctgaaa ggcagcgcgc ggttgatgtt cgccagcagc
    20941 tcctcggcgc tgggctcctc gtcgcgtttg cggccgaagg ggttggcctg ttttttgtcc
    21001 tgcgcgcgcg gcggcagctc gccgggggcg acggcggtct ggatgaggtt ggagacgggt
    21061 tcggccatga tgaccggcat cagatgcggc tgcgcgccgc cgtcaatctg agcatcctgt
    21121 gaataaggcc ggaccggttt tgatcactcc cgtttttggg cgaggagcca gtcccagaac
    21181 tcctggttct cgtaggtttg cgtccaggag tcgtggccca cgccgggata aatggtcagc
    21241 tttgcctggc tgccggcctt tatgagcgcg tcatggattt tcgtcgagaa ctccagcggc
    21301 acggccttgt cctggtcgcc gtggaagatc cagcagggca ggtccttgat gcgctccgcc
    21361 atcacccagc gcacgcccgc gccgccgcag atgggggcga tggccgcgta ggtctccggg
    21421 tgctccagcg ccgtctccca ggtgccaaag ccgcccatgc tcaggcccgt gaggtaaatg
    21481 cgcctcgtgt ccacgcgcag ggtcgcggcc aggtggtcgg tcaccgcttt gactccgtgg
    21541 ggatcccaaa ttcgcgcctt ctcgcattgc aggctcgcca cgatggcggg cagcttttgc
    21601 ccggcctcga ccagcttcgg cgggccgtgc tttttgagca gttccaagtc cgtgccgcgc
    21661 tcgcc
//
LOCUS       DAOMYJ010000073        21642 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Prosthecobacter sp. isolate AMR_MDS_5629
            NODE_859_length_21642_cov_20.490805, whole genome shotgun sequence.
ACCESSION   DAOMYJ010000073 DAOMYJ010000000
VERSION     DAOMYJ010000073.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867850
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prosthecobacter sp. (wastewater metagenome)
  ORGANISM  Prosthecobacter sp.
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Verrucomicrobiaceae; Prosthecobacter.
REFERENCE   1  (bases 1 to 21642)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 21642)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.30
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:34:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,742
            CDSs (total)                      :: 4,684
            Genes (coding)                    :: 4,676
            CDSs (with protein)               :: 4,676
            Genes (RNA)                       :: 58
            tRNAs                             :: 55
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21642
                     /organism="Prosthecobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_859_length_21642_cov_20.490805"
                     /isolate="AMR_MDS_5629"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1965333"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2710
                     /locus_tag="PLP58_09655"
     CDS             <1..2710
                     /locus_tag="PLP58_09655"
                     /inference="COORDINATES: protein motif:HMM:NF015722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HRJ08823.1"
                     /translation="PAAGPGAALPDEARPQRPGGKTPQPTVGADPASKKPSPLLWTLA
                     AALLATGGWLYLDRAPEGATEPEGALTALTTGWQDLLPALAATPLQSATWSATADGSL
                     TCDTPRENSTTVLHSPPIPACQVRVRFASTDFRNIVVFLPTPAGTITFGINKYRRKVG
                     PFNVTGPTMDSVPLADIVLDDGKDHEFLVEMTPARLTARIDGALAFEKDRPDYSQILS
                     PSGYPPIRGSVLGLGAYYGKVVFKACEARSPPAETEAGASAPKTLEEALLNYHWEWTH
                     APLPPDKLTFHADGTATCQRFTWKQWQITGARTARASRAVVDGKELADWEANLYFDDS
                     YSTLHAYSVKGSRHYTGRRMARRDNGHSAASKNEQASSPPPDAATKDLPFINSLGMKF
                     VPVPGTRVLMCVHETRRQDYAAFAEGNPKTDPSWRLVAFRGHQVGETDDHPVVNVSWL
                     EARAFCQWLGEKESLTYRLPRDREWSHAVGIADREDPAQEPGAIKNLVDNLWPWGGDF
                     PPKARLANLADMDLLDAVKDYRVLRGYRDGHAMTAPVMSYPANALGIHDLGGNVWEWC
                     EETYGPEFPSQRILRGGSFLTSVRDWALASHRTPRDPVSSRSLDHGFRCVLELGGEAP
                     PTPEETAAPAGVQASAVPSPAAPPARPLPAATKAEPFTNTLGMKFVPVPGTQVLFCIH
                     ETRRQDYAAYAETIPGVPANWRVAAKGGHLVSTEDDHPVVNVNQGDALAFCAWLGRKE
                     NRRYRLPTDREWSCAAGIGHLEPPHATSRSLHQGLRNLRYWGTEWPPARNNIGNYSDK
                     SYKALFPQANAIDDGRSYDDGHALIAPVMSYPPNEFGIHDLDGNVWEWCQDFFESPTS
                     ANRGLARGSYWAAYNPGQLLVTHRQTGHGRTRCADGGFRIVLEAGQ"
     gene            2962..3540
                     /locus_tag="PLP58_09660"
     CDS             2962..3540
                     /locus_tag="PLP58_09660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009960785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ08824.1"
                     /translation="MNSELPSDETCLKGTRDWPHAPPHRLAEAGVYFVTARTRGQAPL
                     FHTPRRRDWFLALLLELFHSQGWRLEAWAVLSNHYHVVAHSPAGGACSLGPLLQKLHS
                     LATKRLNTEDSTPGRGRLWQNFRETRLTYQESYLARLNYVHQNAKHHGLVTVASQWPW
                     CSAAQFKAAATPAWVRTVAGFGFDEIAKADGE"
     gene            4387..5055
                     /locus_tag="PLP58_09665"
     CDS             4387..5055
                     /locus_tag="PLP58_09665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ08825.1"
                     /translation="MKPLLWDSTETDPWTGQPYTRDSPNPNVTWDGILETGDPGYTPP
                     PASFSNRNRQRASKMKHNSYYPDGIPEQLLWLANFFNKLPAVVPVLPGALQRIFSLVQ
                     VIQEAPAYTDSIATDLGTLGSEDTAPDFNTLQPLIKAVVRADGVFIDWNWGGQRAHLS
                     MIQLQVDRGQGWTDLAFDTTPGYLDTHPRPAALTQWKYRGIYRVGDTPVGLWSAEASA
                     VVGG"
     gene            5271..13325
                     /locus_tag="PLP58_09670"
     CDS             5271..13325
                     /locus_tag="PLP58_09670"
                     /inference="COORDINATES: protein motif:HMM:NF012200.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choice-of-anchor D domain-containing protein"
                     /protein_id="HRJ08826.1"
                     /translation="MTLDETGQVEDHSFWGSEMPWADLGVVTAIAAGAYHSVALNDDG
                     TVVAWGEDNPFENVGQADVPSGLSDVEAIAAGWYHTVALKDDGTVVAWGATDGDVQYA
                     GQTDVDGLTGVKAIAAGGFQTLVLFNNGTVQQYGWGSESPPAGLTGVAAIAIGYRNAA
                     ALKTDGTVVVWGDNDHGQKTIPPGLSNVTSIAVGDEHIVALKADGSVVAWGYNFDGQA
                     TVPAGIGPAFAIAAGGDFTVALLEDTPPQIFPASRNVTAAGGAFDFSITANAGWTWDK
                     SAGSDWVTSSEAASQNGDQTFSYTVAPNTSTTPRSATITIENAVGYHSSTHTIQQEAA
                     ATGSGMVVAWGNNSSGQTNVPAGLNDVTAIAAGNAHTVALKADGTLLSWGLNISGQPA
                     GLNNVIAISAGAEHTVALKSDGTVTAWGFNGSGQTTVPAGLSNVTAIATGWDHNLALK
                     SDGTVVAWGNNSHGQRVVPAGLSGVVAVAAGWKHSVALKSDGTVVAWGYNVDGQTNVP
                     AGLNGVTAIATRGNHTLALKSDGTVVAWGSNSEGQTTVPAGLAGVTTIAAGYNHSLAG
                     KADGTVVAWGGNFYGQTTVPAGLDDVVALAGGAYHSVALVHGTTSAELSVTPTIYNAT
                     AAGGNSSFEVTASGTWSWSKSAGADWVTTSESSNQTGNQTFSYTVATNSDTAQRSAVI
                     TLTSGALTATHTINQAGTESRVIALSGDLAFGNVTVGQSATRTLTIQNTGNSPMSVSG
                     ISYPTGFSGNWSSGSIAAGGSQNVTVTFSPTAAQSYSGNVTVNSNATSGAGVMAISGT
                     GVPGGGDSNVVAWGYNADGQTDVPAGLMDAIAISAGGIHTMALRSNGTVVAWGSNTFG
                     QTNVPAGLTNVTAIAAGGNHSLALKGDGTVVAWGNNSHGQTNVPAGLVNVIAIAASSS
                     SDGTAYHSVALKSDGTVVAWGDNFSGQTNVPAGLANVTAIAAGSVHTMALKSDGTIVV
                     WGPDIEDGPGDVPPGLSGVTAIAAGASHMLALKSDGTVVAWGGWPGSTATQVPAGLSG
                     VVAIAAGGNHSMALKQDGTVVAWGEGMYGQTVPPEGLSGVAAIAAGSNHSVALIGENT
                     SPNLSLTPSFQIFAATGSVFEFAVTASGNWTWSKSGASDWVTTSEGTSQNGNQTFSYM
                     VTANPGTLPRTVVITLTSGMLTASHTIHQMGASTVGGQVIAWGSNGGGQTNVPPGMND
                     VISVSAGNFHTVALRVGGTVAAWGGNSFGETQVPAGLANVIAIAAGSFHTVALKSDGT
                     VVAWGRNTDGQCSVPAGLNNIVAIAAGSDHTLALKSDGTLVAWGANDYGQSSIPPDTV
                     NIIAIAAGAAHNVYLRSDGLMGVWGNNEEGQVNLPGGTGEVKSIAAGMFHSLAARSNG
                     SVVAWGSNWAGQTIVPAGLGDVVQVAAGAAHSLALREDGTVVAWGYDGDGQATVPPSL
                     GGVFAIATGFYHSVAVTAGDAPTAAISLAGNLSFGNVQTGQTATRVLTIQNTGNASLN
                     VTTISYPIVFLGDWNGGSIPPGGSQSVTVTFSPAAVQSYSGTITVISDAASGVNTIAA
                     SGTGIPPNEPPDWQPPVGKENSMTVYAQVENAGVRIDAPGSMLAVFEGGAVAGVASPI
                     NGPAGLFYQLTVWSNQAAVSNLPLKVYDAATNEIHDIQERVNFTANGVLGLINAPVIF
                     TVQPPVVEQVVPLVTGWNWISFNALPETPTVTAVLAGYTPQDNDLIKGVAGSATYFGG
                     SWFPASFAIQPGRMYMLRRQQAGAGALNVTGPPADESLMLNLVAGWNWLGYIPQEGRG
                     LGATLDSLTLADNDLVKSQQDGTATFFGGQWFPGTVMMQPGRGYLLRLAQAQSFAYDG
                     TNGALSAPLALEPEASGTASADPGWMAPLGKENSMTVYAAIQMDGAPVTSIGSLLAAF
                     DGAEVAGVAEVMDGPAGKLFQLTIWSDSASKPGLALKVYDAATGAVRDIAEPVDFATN
                     AILGGIATPLAFNAVPVAPSEFEFSSPVFNASGNPGSVDLEIVRVGGASPATVTLTTS
                     DGSAGMVPPFAAAVAGTDYTALNTVVNFAEGEMSKVVPVTLLPRTGKLPNRRLNAALS
                     APSGGATLGAIASAEVRLLAPDSTKPALAVTLPSASAKTVALPLTFLIFGTVGDALGV
                     DRVVLEYDGDSYSIFPAQGGAANLMALPWSIEFTPKAEGPVSLTFTAYDLAGNSTKVT
                     RSFVYERRQELVVARDVPAALETMPDKAGTVQVKALIAKDAALISKPNPGGLPASVIP
                     PSWAVKAGAQMTLTATAKTGHVFSHWEGLPAGTVQQGHVAVFAMPAQDVPGLTAVFIA
                     NPFTQGAFAALSTKPLFKGLLRPDEETTPGNDTVGVLSATLTASKGSLSGKMWMGGLV
                     VSFTGALHGDGTVWLKADKVMAPALPFAGRELAMSWSETGLAMTVTGPGGEVSEGMAR
                     PPLYSKTNPLRGSLLDVKGRQGYFTIALPAMNAEGPQGSGHAALTLLSDGTLKLAGTL
                     AEGTKVTAAGFLASGDESEVYIVLPTPGGTTKQGSLLGTLVFDETHSDSDVSSADMQW
                     FRPAALTKATVAQAYRAGWPEGIPLGLTGALYDKTATVQTTLGIAGPGRLLFQDGKLA
                     LPVEVTNFIISGSTVTKAPKTDKSFTLSFTAGTGLMKGTFTPGWTSARLPAFQGVLLG
                     KGANQGGWGFFLSNQTGDLNPESGSVLLVK"
     gene            13691..14977
                     /gene="eno"
                     /locus_tag="PLP58_09675"
     CDS             13691..14977
                     /gene="eno"
                     /locus_tag="PLP58_09675"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009962415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004634 - phosphopyruvate hydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HRJ08827.1"
                     /translation="MDDLTIVAVTGREVLDSRGNPTVEVDVMLEGGAIGRAAVPSGAS
                     TGEHEALELRDGDKKRYLGKGVLKAVDAVNNEIADCIIGSNAADQVGLDKAMLEIDGT
                     PTKSKLGANAILGASLAAAKAAATALGLPLYKYVGGPNAKVLPVPMMNIINGGAHSDA
                     PIDFQEFMIMPKGAPSFSEALRYGAEVFHALKKILHDLGLNTAVGDEGGFAPTLKSAS
                     HALEVIAQAIEKAGYKMGEDIFIALDVASSEFFDKAKSKYVFKKSDKSERTAAELVAY
                     YAELKKNFPIISIEDGCAENDWEGWAILTKELGASTQLVGDDLFVTNTKFLQKGIDQS
                     IANSILVKVNQIGSLTETLDAVELAHRHGYTAVMSHRSGETEDYTIADLAVATNCGQI
                     KTGSLSRSDRIAKYNQLLRIEQELGESAVFGGRMKV"
     gene            15001..15333
                     /locus_tag="PLP58_09680"
     CDS             15001..15333
                     /locus_tag="PLP58_09680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ08828.1"
                     /translation="MTHDDFSIEEAPPSQIERWTRTLIGVAKFCILLLAVPVIIRIHE
                     MPLAEQNAMRAKLEEMKAGRDLLLARRDMLLRQVEWIKNDTGYLEIRARDHDEVHKKG
                     EYVIRFED"
     gene            15337..15870
                     /locus_tag="PLP58_09685"
     CDS             15337..15870
                     /locus_tag="PLP58_09685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ08829.1"
                     /translation="MRFAFFLLAPLLLVQCVDPYGNPMPMFGPYTPPPYSDGREQQRS
                     QAREQDQRLYSEAYERGRADGWNDARSGMARNPARGAQGYAPAHATAYQNGYLEGFGA
                     SQPAAQPPYIPPGVQPPAQQPAPVNDPAYNQGYDYGLRDRVAGRPRDPDAHVGRYDPR
                     YRRSFERGYQDAYDSAR"
     gene            complement(16007..16909)
                     /gene="argF"
                     /locus_tag="PLP58_09690"
     CDS             complement(16007..16909)
                     /gene="argF"
                     /locus_tag="PLP58_09690"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018969639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004585 - ornithine carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HRJ08830.1"
                     /translation="MKHLLSLEELTAAEIESLLEQAAVLKKSRHASPQVLTGQQWALI
                     FSKSSTRTRVSFEVGVRELGGQSMFLSSADIQLGRGEPIRDTARVLGRMVHGAIIRTF
                     AQHDVADFAAYSGLPTINALTDDEHPCQILADLQTIAERFGSWKGRRVSFLGDGDCNV
                     ARSWIWASARLGFELVIGAPKEFQPAADFMARVPGGTVHVTDDPRAAVEGVDVLYTDV
                     WVSMGKEEEAAARIETLRPWQVNAALLGQAKPGAIVMHCLPAYRGKEITEEVLEAQAD
                     VIFDQAENRLHAQKAVLVALAKNH"
     gene            complement(16952..18253)
                     /locus_tag="PLP58_09695"
     CDS             complement(16952..18253)
                     /locus_tag="PLP58_09695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009960526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HRJ08831.1"
                     /translation="MADDTEFDPAWLEQYVAPNYGRFPIWPERGEGPWLWDREGKKYL
                     DFAGGVAVCPLGHAHPELAEALARQARTLIHVSNWYCIRQQAELARMIVEEFVRIPGK
                     CFFCNSGAEANEGMIKLARKYGAATPLADGTPRYEIITFTGSFHGRTFGAMSATAQEK
                     IHGGFGPIVPGFVYAPFNDAAALKAAITERTVAIMLEPVQGESGVQPVSAEFLRAARA
                     LCDEHDLLLLLDEVQCGLGRTGESCGWRSIVPGDEIVPDVISWAKSIGSGYPLGAFWI
                     RDREIRNPQSAIRNLPSLLGPGSHGTTYGGSPLACVAGLTTLGIILRDGLIARSATLG
                     RRIAGEVATWRHPLLTDIRAFGMMIGFELDEARLAGMEKVRASGKPPSIFAAWELLQA
                     GLLVVPAGPKVVRWLPPMNLTDAESAEGLRIFKAVLDALAA"
     gene            complement(18302..19147)
                     /gene="argB"
                     /locus_tag="PLP58_09700"
     CDS             complement(18302..19147)
                     /gene="argB"
                     /locus_tag="PLP58_09700"
                     /EC_number="2.7.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018290623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003991 - acetylglutamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetylglutamate kinase"
                     /protein_id="HRJ08832.1"
                     /translation="MNAAEKASVLLEALPYMQGFRSHTFLIKVGGSAMEDPAQVDLFL
                     RDVVFLEAVGINPVIVHGGGKAISKAMQESGLEARFINGMRVTDEETIKIVEQTLARV
                     INPGIVEKINTFGGKAVGLPGTEVFIGEKMKGDLGWVGEVNDCRTGLIQAAVAGEFVP
                     VVSPVARETASGRTLNVNADLAACALATQLKATKLIFLSDVRGVLRDPKDESTLIPTL
                     DPDSITQLKAEGVIGGGMIPKVDSSLASLRGGVGKVHLIDGRIPHALILEIFTDVGIG
                     TEIHL"
     gene            complement(19294..19524)
                     /gene="rpsU"
                     /locus_tag="PLP58_09705"
     CDS             complement(19294..19524)
                     /gene="rpsU"
                     /locus_tag="PLP58_09705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009959650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HRJ08833.1"
                     /translation="MPEVQIRKGEPVDRALKRLKTKLEMEGILEEMRRLRSHETAKER
                     TKRKARAAAKRGKIRFRFTMPKPAADAPVSPV"
     gene            complement(19623..19778)
                     /locus_tag="PLP58_09710"
     CDS             complement(19623..19778)
                     /locus_tag="PLP58_09710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ08834.1"
                     /translation="MSWEQFKRLVIRNWREKLVAVLLAFLFWFMIKSQISSGSRPLPP
                     LPPLRTE"
     gene            complement(19759..20568)
                     /gene="cdaA"
                     /locus_tag="PLP58_09715"
     CDS             complement(19759..20568)
                     /gene="cdaA"
                     /locus_tag="PLP58_09715"
                     /EC_number="2.7.7.85"
                     /inference="COORDINATES: protein motif:HMM:TIGR00159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004016 - adenylate cyclase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diadenylate cyclase CdaA"
                     /protein_id="HRJ08835.1"
                     /translation="MWNFWVQHWRDAVEIIILAVLAYHGYLFFRATRGARILTGLLML
                     LLGLALVSQLLELEVINWLLQRISVFLAVALVVLFQPELRRVLAELGSHRVFSYNRPD
                     PASLDVLIESMRQLSARRCGALFALKRDIDLKPFAETGIETDARLSAELITTIFHPKT
                     ALHDGGAIIEQGRLKAAGCVFPVTQKEVRDRAIGLRHRAAMGISEETDAIALVVSEET
                     GALSICHRGRLEHDLEPEALRARLNQILNTDADAPRDEPPTEETTRELGTI"
     gene            complement(20656..21486)
                     /locus_tag="PLP58_09720"
     CDS             complement(20656..21486)
                     /locus_tag="PLP58_09720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ08836.1"
                     /translation="MEGFLWLLTQMLVPLTAAAAVFLTLGWRWGRRSVDPTVDGLSRR
                     VDAEDQDAAALRREIESLRGVKAALHETRARLDLELKESQERETRLQKEVLRLADERP
                     AAAKPAARGSAVVDAKAKLASLKQLLQTLKERMAALRAEEDVWRQQEAASQGKGGDRR
                     ALARARKKLERVESESARLAREHAALAGQAGFLENALSTTVEQPDDLTRVSGIKEVTA
                     RHLREHGIRSLRQIAAWTEADLDAAGALLAVKHRPRREHWVEQARRLLEQDETGKPAD
                     "
BASE COUNT         3940 a         7047 c         6954 g         3701 t
ORIGIN      
        1 ccccgccgcc ggtcccggag ccgccctgcc agacgaggcc cgcccgcagc ggccaggtgg
       61 caagacgccg cagccgaccg tcggcgcaga cccagcctcg aaaaagccat ccccgctcct
      121 atggaccctc gccgcggccc tgctcgccac cggcggctgg ctttacctgg accgcgcccc
      181 cgaaggcgcg accgagccag agggagcgct cacagccctc accaccggat ggcaggacct
      241 gctgcccgcc ctcgccgcca cccccctgca atccgcgacc tggagtgcca ccgctgacgg
      301 cagcctcacc tgcgacaccc ccagggaaaa ctccaccacc gtcctccaca gcccgcccat
      361 ccccgcctgc caggtgcgcg tccgcttcgc ctccaccgac ttccgcaaca tcgtcgtctt
      421 cctccccacc cccgccggca ccatcacttt cggcatcaac aaataccgcc gcaaagtcgg
      481 ccccttcaat gtcaccggtc caacgatgga cagcgtcccc ctggccgaca tcgtcctcga
      541 cgacgggaag gaccacgaat tcctcgtcga gatgacaccc gcccgcctca ccgcccgcat
      601 agacggcgcg ctggcctttg aaaaagacag gccggattac tcccaaatcc tctccccatc
      661 cggctatccc cccatccgcg gcagcgtcct cggcctcgga gcctactatg gcaaagtcgt
      721 cttcaaagcc tgtgaagcgc gcagcccgcc tgctgaaacc gaggcgggcg cctccgctcc
      781 caaaaccctt gaggaggctc tattgaatta tcactgggag tggactcatg cgcccctgcc
      841 accagacaaa ctcacctttc atgccgatgg caccgccacc tgccagcgct tcacctggaa
      901 gcaatggcag atcaccggcg cgcgcaccgc ccgcgcaagc cgtgcggtgg tggatggcaa
      961 ggagctggca gactgggagg cgaacctcta ttttgacgat tcctacagca cccttcacgc
     1021 ctactcggtg aagggcagcc gccactacac cggaaggcgg atggcacgcc gtgacaatgg
     1081 gcactctgcc gccagcaaga acgaacaggc ttcttctccg ccgcccgacg cagccaccaa
     1141 agacctcccc ttcatcaaca gcctcggcat gaaattcgtc cccgtgcccg gcacccgcgt
     1201 gctgatgtgc gtccacgaga cccgccgcca agactacgcc gcctttgccg agggcaatcc
     1261 caaaaccgac ccgagctggc gcctcgtcgc cttccgcggc catcaagtcg gcgagacgga
     1321 cgaccacccc gtggtgaatg tgagctggct ggaggctcgc gccttctgcc agtggctcgg
     1381 cgaaaaagag agcctcacct accgcctccc gcgcgaccgc gaatggagcc acgccgtcgg
     1441 catcgccgac cgggaggacc cggctcaaga gcctggcgcc atcaaaaacc tcgtggacaa
     1501 cctctggccc tggggcggcg acttcccgcc caaggcccgt ctcgccaacc tcgccgacat
     1561 ggaccttctc gacgccgtca aagactaccg cgtcctccgc ggctatcgcg acggccatgc
     1621 catgaccgcc ccggtcatga gctaccccgc caacgccctc ggcatccacg acctcggcgg
     1681 caacgtctgg gaatggtgcg aggaaaccta cgggcccgag ttccccagcc agcgcatcct
     1741 gcgcggcggc agcttcctca ccagtgtgag ggactgggcg ctcgcctccc accgcactcc
     1801 gcgcgatccc gtcagcagcc gcagccttga ccacggcttc cgctgcgtcc tcgagcttgg
     1861 cggcgaggct cccccaacgc cggaggaaac agccgcgcct gctggtgtcc aggcttcagc
     1921 cgtcccctcg cctgccgcac ctcctgcccg gcctcttccc gccgccacca aagcagagcc
     1981 cttcaccaac accctcggca tgaaattcgt ccccgtgccg ggcacccagg tcctcttttg
     2041 catccacgag acccgccgcc aggactacgc cgcctatgcg gagaccatcc ccggcgtgcc
     2101 agccaactgg cgcgtggcgg ccaagggcgg ccacctcgtc agcaccgagg acgaccaccc
     2161 cgtggtcaac gtcaaccagg gcgacgccct cgccttctgc gcctggctcg gccggaaaga
     2221 aaaccgccgc taccgcctgc ccaccgaccg cgagtggagc tgcgccgccg gcatcggcca
     2281 cctggagccg ccgcacgcca cctcccgctc cctgcaccag ggcctgcgca acctgcgcta
     2341 ctggggcacc gaatggccgc cggcccgcaa caacatcggc aactactccg acaaaagcta
     2401 caaagccctt tttccccagg ccaacgccat agatgacggc cgcagctacg atgacggcca
     2461 cgccctcatc gccccggtca tgtcctatcc gcccaacgag ttcggcatcc acgacctgga
     2521 tggcaacgtc tgggagtggt gccaggactt tttcgaaagc cctacgagtg cgaaccgcgg
     2581 cctcgcccgc ggcagctact gggccgccta caaccccggc cagctcctcg tcacccaccg
     2641 ccagaccggc cacggccgca cccgctgcgc cgacggcggc ttccgcatcg tcctggaagc
     2701 cgggcagtag ccgccccccc tggactgcgg cggcctgccg ccgctttcgg ctcgggcagc
     2761 cctgctgccg ggagtgcgcg ctctttttcc cctcgactcc ctctctccag agacaagtgg
     2821 cggcatccgc cgcatcagtg cggccagcag ggctagcctc aggaaagcgg cagcagggct
     2881 gccgcagtcc aaggtccaag aacttcgacc agggacttcg tcgggcttga cgtccgcctt
     2941 cggtcccgat aaaaccggat catgaattcg gaactgcctt ccgatgagac ctgcctcaaa
     3001 ggcacgcggg actggccgca tgcgccgccg caccggctgg cggaggcggg ggtgtatttc
     3061 gtgacggcgc gcacgcgcgg gcaggcaccg cttttccaca caccgcggcg gcgggactgg
     3121 ttcctggcgc tgctgctgga gctgttccac tcccagggtt ggcggctgga ggcgtgggcg
     3181 gtgctctcga atcactatca tgtggtggcg cacagcccgg cgggcggggc gtgcagcctg
     3241 gggccgctcc tgcaaaagct gcacagcctg gccaccaagc gcttgaatac ggaggacagc
     3301 acgccgggcc gggggcgctt gtggcagaac ttccgggaga cgcgcctgac ctatcaggaa
     3361 agctacctgg cgcggctgaa ttatgtgcat caaaacgcga agcatcacgg tctggtgact
     3421 gtggcctcgc agtggccctg gtgcagcgcg gcccagttca aagcagcagc cacacccgcc
     3481 tgggtgcgca ccgtggcggg cttcggcttc gatgaaatcg ccaaggcgga cggagagtag
     3541 gccgaagccc ccggacgaag tccctggact gcggcagccc tgctgccgct ttcggctcgg
     3601 gcagccctgc tgccgggggt gcgctctctt ttcccctcga ctccctctct ccagaaaaca
     3661 agtggcggct gccccgcatc agtgcggcgc cagccgccac ttcttctctg gaagagcgcc
     3721 gaagcagaaa agaaaatcca cccccggcag cagggctgcc acacctcaaa gcggcggcag
     3781 gccgccgcag tccaaggcag ccgggggcgt cccccttccc gaaaaaaaat tcgccccgga
     3841 ggaaaatttt tttccagccg ctgtaaccag ccgcccggga acccctagag caccctggac
     3901 actcgtgctc cgccccgcct ttcccgccgc ttttccccct ccgctcccca tgcaactcca
     3961 cgtcccaacc cgccactgcg cctccccaga gacgccccgg cggcttcccc ggagcgtttt
     4021 gagacccgtg gaggcgggcg gacccacttt ccagcgtccc tcggacagcg cggaggcgca
     4081 cctccagact tcccagcgtc cctcggaagg tgtggaggtt cgcctccaag gcttcaacgt
     4141 tcgcctccaa agcgtggagg tgagtcacca aggcgtggag gcgcgcctcc agagcgcccc
     4201 aaacacgctt ttgaggccaa aaaacgccat tttcgccctg aaacagcgcc ttggcacccc
     4261 aaaccagccc ggcgcggcca gccacgcgaa aaaatctcac ttcgattatc aactccaccc
     4321 cccaaaaaaa cggtaggacc ctttgaacac acaccatctc cgcccccatg cccgccaccc
     4381 gcccctatga aacccctgct ctgggacagc accgaaacag acccctggac cggccagccc
     4441 tatacccggg acagccccaa ccccaacgtc acctgggacg gcatcctgga gaccggtgac
     4501 cccggctaca cccctcctcc cgcctctttc agcaaccgca accgccaacg cgcctccaag
     4561 atgaaacaca acagctacta cccagacggc atccccgagc agctcctctg gctcgccaat
     4621 ttcttcaaca agctgcccgc cgtcgtcccc gtgctccccg gcgcccttca gcgcatcttc
     4681 agcctcgtcc aggtcatcca ggaagccccc gcttatactg actccatcgc caccgacctg
     4741 ggcaccctcg gcagcgagga caccgccccc gatttcaaca ccctccagcc tctcatcaaa
     4801 gccgtggtcc gcgccgacgg cgtcttcata gactggaact ggggcggcca gcgcgcccac
     4861 ctcagcatga tccagctcca agtggaccgc ggccagggct ggaccgacct cgccttcgac
     4921 accacccccg gctacctcga cacccacccc cgccccgccg ccctcaccca gtggaaatac
     4981 cgcggcatct accgcgtcgg cgacaccccc gtcggcctct ggagcgccga ggccagcgcc
     5041 gtggtgggcg ggtgagcgtc tggccttgcc caaaaccaac aaaccctccc acttccatgc
     5101 tcgctcatct tcgattcggc atttttgcct gcctgctggc cattttatgc atacctcaac
     5161 tccaagcaca ggaccctgtc ggttcggttg tgtcctgggg cgcgaatcat gcagggccac
     5221 ccaatggcca ctttggaatc accgccatcg ccgcaggatg gacacatgtg atgactttgg
     5281 acgagacagg ccaggtggag gatcattcgt tctggggcag tgaaatgccc tgggcggacc
     5341 tgggggtcgt cactgccata gcagcgggtg cttaccactc cgtggcattg aatgatgatg
     5401 gcaccgtggt ggcatggggt gaggataatc ctttcgaaaa tgtcggccag gcggatgtgc
     5461 cttccgggtt gtccgatgtc gaggccattg ccgccggctg gtatcacacc gtcgccctca
     5521 aagatgatgg aacggtggtc gcctggggtg ccactgacgg agacgtccag tacgctgggc
     5581 agacggatgt ggacgggcta actggcgtga aggccatcgc agcaggcggt tttcagacgc
     5641 tggtgctttt caataatggc acggtgcagc agtatggctg gggaagcgaa agcccgcctg
     5701 cgggcctgac gggagtggcg gcgattgcca tcggatatcg caacgccgca gctttaaaga
     5761 cagacggcac ggtggtcgtc tggggggaca acgaccacgg ccagaaaacc attccccccg
     5821 gcctcagcaa cgtcacatcc atcgctgtag gggacgagca tatcgtcgcg ctgaaggctg
     5881 atggcagcgt ggtggcatgg ggttacaact ttgatggaca agctaccgtg cccgcgggca
     5941 tcggccccgc tttcgccatc gccgcagggg gagactttac agtggcgttg ctggaggata
     6001 ccccgccaca gattttccct gccagccgca atgtcacggc ggcaggcgga gcgttcgatt
     6061 tcagcatcac cgcaaacgcg ggttggacct gggacaaaag cgcgggcagc gactgggtga
     6121 ccagcagcga ggccgccagc cagaatggcg atcaaacctt cagctacacc gtggccccga
     6181 acaccagtac cactccgcgc agcgccacaa tcaccattga aaatgccgtg ggctaccact
     6241 ccagcacgca cacgatccag caggaggcag cggcaacagg aagcggcatg gtggtggcat
     6301 gggggaataa cagcagcgga caaacgaatg tcccggcagg actcaatgat gtcaccgcca
     6361 tcgctgcggg caacgcgcac accgtggctt tgaaggcgga cggcacgctg ttgagctggg
     6421 gtttgaacat ttcaggccag cctgcgggtt tgaacaacgt catcgcgatt tcagccggag
     6481 cagagcatac ggtggcgttg aaaagcgacg gtacggtaac ggcctggggc tttaatggca
     6541 gtggtcagac gaccgtgcct gcggggttga gcaatgtcac agccatcgca acaggctggg
     6601 atcacaattt ggctctaaaa agtgacggga cagtggtcgc ttggggaaat aacagtcatg
     6661 gccaaagagt ggtgccggcg ggtttgagcg gcgttgtggc ggtggctgcc ggatggaagc
     6721 actcggtggc attgaagagt gatgggactg tcgtggcctg gggctacaac gtggacggcc
     6781 agaccaatgt ccctgcgggg ctgaatggag tgactgccat tgcaacgagg ggcaatcaca
     6841 ctctggcact caagagtgac ggcacggtcg tggcctgggg cagcaacagt gaggggcaga
     6901 ccactgtgcc ggcagggctg gcgggtgtga ccaccatcgc cgcaggctac aaccacagtc
     6961 tggcaggcaa ggctgatgga accgtggtgg cctggggcgg gaatttttac gggcagacca
     7021 cagttcccgc cggcctggat gatgtggtgg ctttggcggg cggcgcttat cactccgtgg
     7081 cgttggttca tggaacaacc tctgccgaac tctcagtcac cccgaccatt tataatgcga
     7141 cagcggctgg cggaaattcg agcttcgagg tgaccgcatc cggaacctgg agctggagca
     7201 agagtgcggg cgctgattgg gtaaccacct ccgaatcttc taatcaaacc ggaaatcaaa
     7261 ccttcagcta cacagtagcc accaattcag acacggctca gcgcagcgct gtcatcacat
     7321 tgaccagcgg cgcgctcaca gccactcata ccatcaacca ggctggtact gaatcacgcg
     7381 tcatcgccct ctccggcgac cttgctttcg gcaatgtgac cgtgggccaa agcgcgacga
     7441 ggacgttgac gatccagaac actggcaact ccccgatgag tgtgtcaggc atcagttacc
     7501 ccacgggctt cagcggcaac tggagcagcg gcagcatcgc ggctgggggc tctcagaatg
     7561 tgacggtgac tttctcccca accgcagcgc agagctacag cggcaatgtc accgtcaact
     7621 ccaacgccac cagcggagcc ggggtgatgg caatctctgg aacaggcgtt cccggtggcg
     7681 gggactcgaa tgtcgtagcc tggggataca acgctgatgg acagacggat gtgccggcag
     7741 gtttgatgga tgccattgct atctctgctg gaggcatcca cacaatggca ttgcgaagca
     7801 atggcacggt cgtcgcctgg ggaagcaaca ccttcgggca gacgaatgtg ccggcaggct
     7861 tgaccaatgt cactgccatc gcggcaggtg gaaaccattc tctggcacta aaaggtgacg
     7921 gcacggtggt tgcctgggga aacaacagtc atgggcagac aaatgtgcct gcgggcctgg
     7981 tgaatgtgat cgccatcgcc gccagttcct cgtctgatgg gacggcgtat cattcggtgg
     8041 ctctgaaaag tgacggcact gtggttgcct ggggggataa cttctccggg caaacgaatg
     8101 tgcctgcagg tctggcgaat gtgacagcca ttgcggcagg ctccgtccac acgatggcgc
     8161 tcaaatccga tggcacgatc gttgtgtggg gaccagatat cgaggacggc ccgggtgatg
     8221 tgcctcctgg tttgagtggt gtcactgcga ttgccgcagg tgcgtctcac atgctggcgt
     8281 tgaaatccga tggcacagtg gtggcatggg gcggatggcc cggaagcacc gcgacgcagg
     8341 ttcctgccgg tctgagcggt gtggttgcca tcgcagcagg tggaaaccac tccatggctc
     8401 tcaaacaaga tggcaccgtg gtggcatggg gagagggcat gtacggccag actgttccac
     8461 ccgagggctt gagcggggtg gcggccattg ccgcaggctc gaatcacagc gtggcgctga
     8521 tcggcgaaaa cacgtctccc aacctgagct tgacaccttc attccaaatt tttgcggcca
     8581 cagggagcgt ttttgagttt gcggtgacgg cttccggcaa ctggacctgg agcaaaagtg
     8641 gggcaagcga ttgggtgact acttctgaag gcacaagcca aaatggcaat cagaccttca
     8701 gctacatggt gaccgcgaat cctggaacat taccgcgcac ggttgtcatc acattgacca
     8761 gcggcatgct cacagcctcc cataccattc atcaaatggg cgcttccacg gttggcgggc
     8821 aggtgattgc ctggggtagc aatggcggag ggcagaccaa tgtgccgcca gggatgaatg
     8881 acgtgatttc tgtctccgcc ggcaatttcc acacggtggc actgcgtgtg ggaggcaccg
     8941 tggcagcctg gggaggcaat tcctttggtg aaacccaggt gcctgctgga ttggcgaacg
     9001 tcattgccat cgctgctggt tcctttcaca cggtggcact caagagtgat ggcacggtgg
     9061 tcgcctgggg tagaaacacg gacggccaat gctccgtgcc cgccgggtta aacaatatcg
     9121 tggctatagc cgctggttct gaccacactc tcgcattgaa gagcgacggc actcttgttg
     9181 cctggggggc caatgattat ggccagtctt ccatcccgcc agacacggtg aacatcatcg
     9241 cgatcgcggc aggggcggcg cataatgttt atctgagaag tgacgggctt atgggtgtct
     9301 ggggcaacaa tgaggaaggg caggtcaatc ttccaggcgg gacaggggag gttaagtcca
     9361 tcgcggctgg catgtttcac agtttggctg cgcggagtaa tggttccgta gtggcatggg
     9421 gcagcaattg ggccggtcag acgattgtgc ccgcgggttt gggcgatgtc gtccaagtgg
     9481 cagcgggggc cgcgcattca ttggcgctga gggaggatgg cacagtggtt gcctggggat
     9541 atgacggaga tggtcaggca acggtgcctc cgtctctcgg cggcgttttt gcgattgcca
     9601 ccggttttta ccattccgtg gcagtgaccg ctggagacgc tccaaccgca gccatctcac
     9661 ttgccggaaa cctttcgttt ggaaacgtgc agacaggaca gactgcgaca cgggttttga
     9721 cgattcaaaa cacgggcaac gccagcttga atgtgacgac gatctcttat ccgatcgttt
     9781 tcctggggga ctggaacggt ggcagcattc ctccaggcgg tagccagagc gtgacagtga
     9841 ctttttctcc ggcagcggtg cagagctaca gtggaaccat cacggtgatc tctgatgcgg
     9901 caagtggcgt aaacacaatc gcggcctccg gcaccggcat cccgccaaac gagccgcctg
     9961 actggcagcc gcccgtcggc aaagagaaca gcatgacggt gtatgcccag gtggagaatg
    10021 ccggggtgcg gattgatgct cctgggagca tgctggcggt gtttgaaggt ggtgctgtgg
    10081 cgggtgtggc ctcgcccatc aatggtcccg ccgggttgtt ttaccagctc acggtctggt
    10141 cgaatcaagc ggctgtcagc aatcttccgc tgaaggtcta tgacgcggcg acgaatgaga
    10201 ttcatgacat ccaggaacgg gtgaacttca ccgccaacgg cgtgctgggt ctgatcaacg
    10261 cgccggtgat cttcacggtg cagccgccgg tggtggagca ggtggtgcca ctggtcacag
    10321 gctggaactg gatttccttc aacgcgctgc cggaaacacc gacggtgacg gctgtgctgg
    10381 cgggctacac gccgcaggac aatgatctga tcaagggcgt ggccggctcg gcgacttatt
    10441 ttggcggttc ctggttcccg gccagttttg ccatccagcc gggcaggatg tacatgctac
    10501 gtcgtcaaca ggctggcgct ggcgcgttga acgtgactgg cccgcctgcg gatgagtcct
    10561 tgatgctgaa cctggtggct ggctggaact ggctgggtta catcccgcag gaagggcgcg
    10621 ggctgggcgc gactctggac agcctgacac tggctgacaa cgatctggtg aagtcgcagc
    10681 aggatggcac ggcgactttc ttcggggggc agtggttccc tggcacggtc atgatgcagc
    10741 caggccgggg ttatctgctg cggctcgccc aggcgcagag cttcgcttat gacgggacga
    10801 atggcgcttt atcggcaccg ctggcgttgg agccggaagc atccgggaca gcaagtgcgg
    10861 atcctggctg gatggcgcct ctgggaaagg agaatagcat gacggtctat gccgccattc
    10921 agatggatgg cgcgcctgtg acgtccattg gcagtttgct ggctgccttt gacggcgcgg
    10981 aagtggctgg tgtggcggag gtgatggacg ggcctgcggg gaaacttttc cagctcacga
    11041 tctggtctga ctctgcgtcg aagccaggtc ttgcgctcaa agtctatgat gctgccacgg
    11101 gagctgtccg tgacattgca gagccggtgg actttgccac caatgcgatc ctgggcggca
    11161 ttgccacgcc tcttgccttc aacgccgttc cagtggcgcc ttcggagttt gagttcagct
    11221 ctccggtctt caatgcctct gggaatcctg ggtctgtgga tttggagatc gtccgagtgg
    11281 gcggtgcatc accggcaact gtgacgctga ccaccagcga cggcagcgcg ggcatggtgc
    11341 cgcccttcgc cgccgcagtg gccggcacgg actacacggc tctcaacacc gtggtgaact
    11401 tcgccgaagg ggagatgagc aaggtagtgc ctgtcaccct tctcccgcgc acgggcaagc
    11461 tgcccaaccg gcgcctcaac gcggccctct cggccccgag tggcggcgcc acgctcggcg
    11521 ccatcgccag cgccgaggtg cgcctgctag ccccggacag caccaagccg gctctcgccg
    11581 tcacactgcc ctccgccagc gcgaagactg tcgccctgcc cttgaccttt ctcatcttcg
    11641 gcaccgtcgg tgacgccctg ggcgtggacc gcgtggtgtt ggaatacgac ggggattcct
    11701 actccatctt ccccgcccag ggtggagcgg cgaacctcat ggcgctgccc tggtccatcg
    11761 agttcacccc caaggcggaa ggccccgtga gcctgacctt caccgcctat gacctcgccg
    11821 gcaacagcac caaagtgacc cgctccttcg tctatgagcg ccgccaggag ttggtcgtcg
    11881 cgcgcgatgt gcccgcggcg ttggagacca tgccggacaa ggcgggcacc gtccaggtga
    11941 aggccctcat tgccaaggac gccgcgctca tctccaagcc caacccgggc ggtctgcccg
    12001 cgtcggtgat cccgccaagc tgggcggtga aggcgggcgc gcagatgacc ctcaccgcca
    12061 ccgccaaaac gggccacgtt ttcagtcatt gggaagggtt gcccgccggt actgtgcagc
    12121 aggggcatgt ggcggtcttc gccatgcccg cgcaggacgt gccgggactc acggcggtct
    12181 tcatcgccaa tcccttcacc cagggtgcct ttgcggccct gagcaccaag cccttgttca
    12241 aggggctgct gaggcccgat gaagaaacca ctccgggcaa tgacaccgtg ggggtcctca
    12301 gcgccaccct cacagcctcc aagggcagcc tcagcggcaa aatgtggatg ggcgggctgg
    12361 tagtctcctt caccggcgcg ctgcacggcg acggcacggt gtggctcaaa gcggacaagg
    12421 tgatggcgcc cgccctgccc ttcgccggtc gcgagctggc catgagctgg agtgaaaccg
    12481 gccttgcgat gacagtgacc ggccccggcg gcgaggtgag cgagggtatg gcaagaccac
    12541 cgctttactc gaagacgaat cctctgcgcg gcagcctgct tgatgtgaaa gggcgccagg
    12601 gctacttcac catcgccctg cccgccatga atgccgaagg gccgcagggc agcggtcacg
    12661 cggcgctgac actgctgagc gacggcacgt tgaagctcgc cggcacgctg gccgagggca
    12721 cgaaggttac ggcggcgggt ttcctggcct cgggtgacga gtcggaggtt tacatcgtgc
    12781 tgcccacgcc gggtggaacg accaagcagg gcagcttgct gggcacgctg gtctttgacg
    12841 agacccactc ggacagcgat gtcagcagcg cggacatgca gtggttccgc cctgcggccc
    12901 tgaccaaggc gaccgtcgcg caggcctacc gcgccggctg gccggagggc atccccctgg
    12961 gactcacagg tgccctgtat gacaaaaccg ccacggtgca gaccaccctg ggcatcgccg
    13021 gaccgggccg gctgctgttc caggacggca aactggcgct ccccgtggaa gtgaccaact
    13081 tcatcatcag cggcagcaca gtgaccaagg cgccgaagac cgacaagagc ttcaccctga
    13141 gcttcactgc gggcacgggc ctgatgaagg gcaccttcac ccccggctgg accagcgcca
    13201 ggctgcccgc cttccagggc gtgctgctgg gcaaaggcgc caaccagggc ggctggggct
    13261 tcttcctcag caaccagacc ggcgacctca atcccgaaag cggcagcgtc ttgctggtca
    13321 agtaactctg ccctctctcg tcgcgactgc gacggggcag ttagcctgaa tattctggtg
    13381 tctccgcgtc atgcgccgtt ggaacggctt gggccgcatt cgacaagctc aaagatggaa
    13441 ttcgttcatc cctgcccgcc gggtttgcat catgcggcag aacgctgcgt gatgcctgcg
    13501 ggtgcgcgac ttgggtgttg tattcaaaat cagtttcggc cacgccgcgg ggggcgggtg
    13561 tgtcgttgcc gtgtcacatc tctggttgag cgccggggct ttgctgctat caagcaggcg
    13621 ctcgggcggg aaattcacct gcctgcttgc caaaaccccg aagattaagc atacttaaca
    13681 aaatcaactc atggacgatc tgaccattgt agcagtgacc gggcgcgaag ttctcgactc
    13741 gcgcggaaac cccaccgtgg aggtggatgt gatgctggag ggcggcgcca tcggacgcgc
    13801 cgccgtgccc agcggcgcca gcaccggcga gcacgaggct ctagaattgc gcgatggcga
    13861 caagaaacgc tacctcggca agggcgtgct caaggccgtg gacgcggtga acaacgagat
    13921 cgccgactgc atcatcggca gcaacgccgc cgaccaggtg gggctcgaca aggccatgct
    13981 ggagattgac ggcacgccga cgaagtccaa actcggcgcc aacgccatcc tcggagcctc
    14041 cctagccgcc gcgaaggccg ccgccaccgc tcttggcctg ccgctctaca agtatgtggg
    14101 cggcccgaac gcgaaagtgc tgccggtgcc gatgatgaac atcatcaacg gcggcgcgca
    14161 cagtgacgcg ccgattgact tccaggagtt catgatcatg ccgaaaggcg cgccgagctt
    14221 ctccgaggcg ctgcgctacg gcgcggaagt cttccacgcg ctgaaaaaga tcctgcacga
    14281 tttgggtctg aacacggcgg tgggtgacga gggcggcttt gcccccacgc tgaagagcgc
    14341 cagccacgcg ctggaagtca tcgcccaggc catcgaaaag gccggctaca aaatgggcga
    14401 ggacatcttc atcgcgctcg acgtggcctc ctccgagttc ttcgacaagg cgaagagcaa
    14461 gtatgtcttc aagaagagcg acaagtccga gcgcaccgcc gccgaactcg tggcctacta
    14521 tgcggagttg aagaaaaatt tcccgatcat ctcgatcgaa gacggctgcg ccgagaacga
    14581 ctgggaaggc tgggccatcc tgaccaaaga gctcggcgcc tccacccagc tggtgggcga
    14641 tgacctcttc gtaaccaaca ccaagttcct ccagaagggc attgaccaga gcatcgccaa
    14701 ctcgatcctc gtgaaagtga accagatcgg ctccctgact gagacgcttg acgccgtcga
    14761 actggcccac cgccacggct acaccgccgt gatgagccac cgctccggcg agaccgagga
    14821 ctacaccatc gccgacctcg ccgtggccac gaactgcggg cagatcaaga ccggctccct
    14881 ctcccgcagc gaccgcatcg cgaaatacaa ccagctcctg cgcatcgagc aggaactcgg
    14941 tgaaagcgcc gtcttcggcg gccgcatgaa agtctgacaa acccgcaccc cggcaccgcc
    15001 atgacgcacg atgatttctc cattgaagaa gcgccgccca gccagatcga gcgctggacg
    15061 cgcacgctga tcggtgtggc gaaattttgc atcctgcttc tggcggtgcc ggtgatcatc
    15121 cgcatccacg agatgccgct ggcggagcag aatgcgatgc gcgccaagct ggaggagatg
    15181 aaggccgggc gcgatctcct tctggccagg cgcgacatgc ttctgcgcca ggtcgagtgg
    15241 atcaaaaacg acaccggcta tctggaaatc cgcgcccgcg accatgatga agttcacaaa
    15301 aagggcgagt atgtgatccg cttcgaggac tgacccatgc gttttgcctt tttcctgctc
    15361 gccccgctgc ttctggtcca atgcgtggac ccctacggca atcccatgcc gatgttcggc
    15421 ccctacacgc cgccgcccta ctccgacgga cgcgagcagc agcgcagcca ggcgcgcgag
    15481 caggatcagc gcctttactc cgaggcttat gaaaggggcc gcgccgacgg ctggaatgac
    15541 gcgcgctcag gcatggcgcg caatcccgcg cggggagccc agggctacgc gcccgcccat
    15601 gccaccgcct atcaaaacgg ttatctggag ggttttggcg cctcccagcc cgccgcgcag
    15661 ccaccctaca tcccgcccgg tgtccagccg cccgcgcagc agccggctcc tgtgaatgac
    15721 cccgcctaca accagggcta tgactacggc ctgcgcgacc gcgtggcggg ccgtccgcgc
    15781 gatcccgacg cgcatgtcgg acgctacgat ccgcgctacc ggcggtcgtt cgagcgcggc
    15841 taccaagacg cctacgattc cgcacgctga ccgcggcaaa gcggcagggg ttttcatggg
    15901 gaacagggac cccgtctgtt cgcgcaggca tggatgcccg cgcgccatgc gccttctgtc
    15961 atcgtgagtc aaactcccgc tgctccgctt gtcaccaccg cgagaatcag tgattcttag
    16021 ccagggccac gagcacggct ttctgcgcgt gcaggcggtt ttcagcctgg tcgaaaatga
    16081 catcggcctg ggcttcgagc acctcctcgg tgatctcctt gccgcggtag gcgggcaggc
    16141 agtgcatgac gatggcgccg ggctttgcct gcccgagcag ggcggcattc acctgccagg
    16201 ggcgcagcgt ttcgatgcgg gcggcggcct cctcctcctt gcccatgctg acccagacat
    16261 cggtgtaaag cacgtccaca ccctccacgg cggcgcgggg gtcatcggtg acgtggacgg
    16321 tgccgccggg cacgcgcgcc atgaagtcgg cggcgggctg gaactccttc ggcgcgccga
    16381 tgacgagctc gaagccgagc ctggcgctgg cccagatcca ggagcgcgcg acattgcagt
    16441 cgccatcccc gaggaagctg acgcggcggc ctttccagga gccgaagcgc tcggcgatgg
    16501 tctggaggtc ggccaaaatc tggcaggggt gctcgtcatc ggtgagcgcg ttgatggtgg
    16561 gcaggccgct gtaggcggcg aagtccgcca catcatgctg ggcgaaggtg cggatgatgg
    16621 ccccgtgaac catgcgcccg agcacgcgcg cggtgtccct gatcggctcg ccgcgaccga
    16681 gctggatgtc cgccgaggag aggaacatgc tctggccgcc cagctcgcgc acgcccactt
    16741 cgaagctgac gcgggtgcgc gtggaggact tgctgaaaat gagcgcccac tgctggccag
    16801 tgaggacctg cggggaggcg tggcggcttt ttttcaaaac ggcggcctgc tccagcaggg
    16861 attcgatttc ggcggcggtg agttcttcga gggaaaggag gtgtttcatg cgggttcgcg
    16921 tttctcagtt cttcagttcg gggttcgcgg ttcaggcggc cagggcgtcg agcacggcct
    16981 tgaagatgcg caggccttcg gcggactcgg cgtcggtgag gttcatcggc ggcagccagc
    17041 gcaccacttt cggcccggcg ggcacgacga gcaggccggc ctggagcagc tcccaggccg
    17101 cgaagatgga gggcggcttg ccggaggcgc gcaccttttc catgccggcg aggcgcgcct
    17161 cgtccagttc gaagccgatc atcatgccaa aggcgcggat gtcggtgagg agcggatgcc
    17221 gccaggtcgc cacctcgccc gcgatgcgcc gcccaagcgt ggcggagcgg gcgatgaggc
    17281 catcccgcaa aatgatgccg agggtggtca gccccgccac gcaggccagc gggctgccgc
    17341 cataggtggt gccgtgggag ccggggccga gcagggaagg gagattacgg attgcggatt
    17401 gtggattgcg gatttcacga tcccgaatcc agaaggcgcc cagcggatag ccgctgccga
    17461 tggacttggc ccaactgatg acatccggca cgatctcgtc accgggcacg atgctgcgcc
    17521 agccgcagct ctcccccgtg cgcccgaggc cgcactgcac ctcgtcgagc aggagcagca
    17581 ggtcgtgctc gtcacacagg gcgcgcgcgg cgcggagaaa ctcggcgctc accggctgca
    17641 cgccgctttc cccctgcacg ggctcgagca tgatggcgac ggtgcgctcg gtgatggcgg
    17701 ccttcagcgc ggccgcgtca ttgaagggcg catagacaaa gccggggacg atggggccga
    17761 agccgccgtg gattttctcc tgcgcggtgg cgctcatggc cccgaaggtg cggccgtgga
    17821 agctgccggt gaaggtgatg atctcataac gcggcgtgcc atccgccagg ggtgtggccg
    17881 cgccgtattt gcgcgccagc ttgatcatgc cctcgttggc ctcggcgccg ctgttgcaga
    17941 agaagcactt gccggggatg cgcacgaact cctcgacgat catgcgcgcc agctccgcct
    18001 gctggcggat gcagtaccag ttcgagacat ggatcagcgt gcgcgcctgc cgcgccagcg
    18061 cctccgccag ctccgggtgc gcgtggccga gcgggcacac ggccacgccg ccggcgaagt
    18121 cgaggtactt tttcccctcg cggtcccaca gccacggccc ctcgccgcgc tccggccaga
    18181 tggggaagcg cccgtaattg ggcgccacat attgctccag ccatgcgggg tcgaattcag
    18241 tgtcgtctgc catcgtgtct gtctctgggg gaaaaggttc gtctgccggg gaatgcgggc
    18301 ctcacaaatg aatctcggtg ccgattccga catcggtgaa gatttccaaa atcagcgcgt
    18361 gcgggatgcg cccgtcaatg aggtggacct tgcccacgcc gccgcgcagg gaggccaggg
    18421 aggagtccac cttcgggatc atgccgccgc cgatgacgcc ctcagccttg agttgggtga
    18481 tggagtcggg atcgagcgtg ggaatgaggg tggactcgtc cttggggtcg cgcagcacgc
    18541 cgcgcacatc gctgaggaag atcagtttcg tggccttcaa ctgtgtggcc agggcgcagg
    18601 cggcgaggtc ggcgttcaca ttcagggtgc gcccgctggc cgtctcccgc gccacgggcg
    18661 agaccacggg cacgaactcg cctgccaccg ctgcctggat gaggccggtg cggcagtcat
    18721 tcacctcgcc cacccagccg aggtcgccct tcattttctc gccaatgaag acctccgtgc
    18781 ccggcagccc gacggccttg ccgccgaagg tgttgatctt ctcgacgatg ccggggttga
    18841 tgacgcgcgc cagggtttgc tcgacgatct tgatggtctc ctcgtccgtg actctcatgc
    18901 cattgatgaa gcgcgcctcc agtccgctct cctgcatggc cttggagatg gccttgccgc
    18961 cgccgtggac gatgacgggg ttgatgccga cggcttcgag gaagaccacg tcgcgcaaaa
    19021 acaggtccac ctgcgccggg tcctccatgg cgctgccgcc caccttgatg agaaaggtgt
    19081 ggctgcggaa gccttgcatg tagggcaggg cttcgagcag gacggaggct ttttcggcgg
    19141 cgttcatggc ggacgggtat ggtaaagaaa atggagaaat ggaaaagcgg gcggttgcgg
    19201 gcaggttggc ggtgccggtc agaaaccaaa aggctgcctg cttgcgcagg cagcctttgc
    19261 gggttggatt ttccctcaac gagccgggga aattcagaca ggggaaacgg gggcgtcggc
    19321 ggcgggcttg ggcatcgtga agcggaagcg gatcttgccg cgtttggcgg cggcgcgcgc
    19381 tttgcgcttg gtgcgctcct tggcggtttc gtgggagcgg aggcggcgca tttcttcgag
    19441 aatgccctcc atttcgagct tggtcttcag gcgcttgagg gcgcggtcca cgggttcacc
    19501 tttgcggatt tggacttcgg gcatggatgt cttgggatgt gtgtgctgtt tgggggaaaa
    19561 aacggcccgc agattaaggg ccgccctccg ggcgtcaacc tgaaagacgg ctaattttcc
    19621 ggtcactcgg tgcgcagggg cggcagcggc ggcagggggc gggagccgct gctgatctgg
    19681 gacttgatca tgaaccagaa caggaaggcc agcaggacgg ccaccagctt ctcgcgccag
    19741 ttacggatga cgagtctctt aaattgttcc cagctcacgg gtggtctcct cggttggggg
    19801 ttcgtcgcgc ggcgcgtccg cgtcggtgtt gagaatctgg ttgagccggg cgcgcagggc
    19861 ctccggctcc aggtcgtgct ccaggcggcc gcggtggcag atggagagcg cgccggtctc
    19921 ctcgctgacc acaagggcga tggcgtcggt ctcctcgctg atgcccatgg cggcgcggtg
    19981 gcgcagcccg atggcgcggt cacgcacttc cttctgggtc acggggaaga cgcagccggc
    20041 ggccttgagc ctgccctgct caatgatggc gccgccgtcg tgcagggcgg ttttggggtg
    20101 gaagatggtg gtgatcaact ccgcggagag gcgcgcgtcg gtctcgatgc cggtctcggc
    20161 gaagggtttg agatcaatgt cccgcttcag ggcgaagagc gcgccgcagc ggcgggcgga
    20221 gagctggcgc atggactcga tgagcacgtc gagcgaggcg gggtcgggcc ggttgtagga
    20281 gaagacgcgg tggctgccca gctcggcgag cacgcggcgc agctcgggct ggaagagcac
    20341 gaccagcgcc acggccagaa agacggagat gcgctggagc agccagttga tcacttccag
    20401 ctccaggagc tgcgacacca gcgccaagcc gagcaggagc atgagcagcc ctgtgaggat
    20461 gcgcgccccg cgcgtggcgc ggaagaagag ataaccatga taggccagca cggccagaat
    20521 gatgatctcg acggcgtcgc gccagtgctg tacccagaaa ttccacatga cgggacacgc
    20581 ttatgaccgc aggcgggcgg gcgcgcaagg gtttcatcca ggccggcgcg catttctgaa
    20641 aaaaagcgcg cgcagtcaat ccgccggctt ccctgtctca tcctgctcca gcagccgccg
    20701 cgcctgctcc acccagtgct cgcggcgggg acggtgtttg acggcgagca gggcgccggc
    20761 ggcgtcgagg tccgcctccg tccacgcggc gatctgccgc agcgagcgga tgccgtgctc
    20821 gcgcagatgg cgggcggtga cctccttgat gccgctgacg cgggtgagat cgtccggctg
    20881 ctccacggtg gtggagaggg cgttttcgag aaagccggcc tggcccgcca gtgcggcgtg
    20941 ttcgcgcgcg aggcgggcgg actctgactc gacccgctcc agttttttgc gggcgcgggc
    21001 cagagcgcgg cggtcgccgc ctttgccctg ggaggccgcc tcctgctgcc gccacacgtc
    21061 ctcctcggcg cgcagcgcgg ccatgcgctc cttcagcgtc tggaggagct gcttgaggct
    21121 ggcgagtttt gcctttgcgt ccacgacggc gctgccgcgg gcggcgggtt tggcggcggc
    21181 ggggcgctcg tcggcgaggc ggagcacttc cttttgcagg cgcgtctcgc gctcctggga
    21241 ctccttcagt tccaggtcca ggcgcgcgcg ggtctcgtgg agggcggcct tgacgccgcg
    21301 cagactctcg atctcccggc gcagcgcggc cgcatcctgg tcctcggcgt ccacgcgccg
    21361 gctgaggccg tccacggttg gatccacgct gcggcggccc cagcgccagc ccagggtgag
    21421 aaagacggcc gccgccgcag tgagcggcac gagcatttgg gtcagcagcc agagaaatcc
    21481 ttccatgaat cagggggcgg cctcctttgt ggcatggttc acggcattgc gccagtgatg
    21541 acgcaggtgg tcgaagcctt cggcaaaagt gtcctcggcc atggcggcgg ggaagtcctc
    21601 cggcagaagg gggcttcccg ccagcgcgcc gggggccagc ag
//
LOCUS       DAOMYJ010000074        21635 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Prosthecobacter sp. isolate AMR_MDS_5629
            NODE_860_length_21635_cov_21.060565, whole genome shotgun sequence.
ACCESSION   DAOMYJ010000074 DAOMYJ010000000
VERSION     DAOMYJ010000074.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867850
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prosthecobacter sp. (wastewater metagenome)
  ORGANISM  Prosthecobacter sp.
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Verrucomicrobiaceae; Prosthecobacter.
REFERENCE   1  (bases 1 to 21635)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 21635)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.30
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:34:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,742
            CDSs (total)                      :: 4,684
            Genes (coding)                    :: 4,676
            CDSs (with protein)               :: 4,676
            Genes (RNA)                       :: 58
            tRNAs                             :: 55
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21635
                     /organism="Prosthecobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_860_length_21635_cov_21.060565"
                     /isolate="AMR_MDS_5629"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1965333"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..981)
                     /locus_tag="PLP58_09725"
     CDS             complement(<1..981)
                     /locus_tag="PLP58_09725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ08837.1"
                     /translation="MKTRRHFLAGLGSAAAATWTQTLRAAPDPKKDVVIGHGSHRYKV
                     VKDWVAPGQGRYHPILNCHEMVQVKDGRLFMVGDHWDNQMLVFKPDGTILDSWGSVWP
                     GGHGLTLSEENGEEFLFVTDSGLWKSGSGGYRQTGRVAKIDLAGREIFSISHPMSVGA
                     YEPEMFFNPTEVAIAPNGDIYVVDGYGSQFVLRYDARGKFLQRFGGKDGVPAEQRLNN
                     AHGIAIDTRQGADKATVIVTSRADNCFRRFSLDGQYLGTIPVPGAMVCRPVIAGQEIH
                     AGVCWSKTPEINKLPQNPSGFTVILGADDRVISAPGGTAPVYEDGKLQPLM"
     gene            complement(1016..2254)
                     /locus_tag="PLP58_09730"
     CDS             complement(1016..2254)
                     /locus_tag="PLP58_09730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009961076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HRJ08838.1"
                     /translation="MKSPAAKKLFVMMVLEFFIWGAWLPLIWGYMGADGLKFTDLQIT
                     WVGSAFAIASVLGIFFSNQFADRHFAAEKFMSFSHLVGGLAILGMYWAKDFPTFFGLM
                     LVHSLLYVPTISVSNSIAFTHLKDAQKEFGLVRMGGTIGWILAAWPLYFILEGKTGAD
                     AIAARANIFLISGAASIALSLFSLSLPHTPAKPASKGESNFAWLKAAMFLKKPFLLIL
                     FIVTFIDATIHNGYFLLAGNFLGSDVVGIEQKWIMPVMSIGQIAEILTMAVLGWFLSR
                     LGWKTTMILGILGHAARFAVFAFMPENQTMIIAVQVLHGICYAFFFATLYIFIDAAFP
                     KDARSSAQGLFNLLVLGLGDLAAKWFFIPLQGKLTVDGVVNYKELFLWPTGLSLAAAV
                     LLLIAFFPPKQLAPPEEVTH"
     gene            2801..3304
                     /locus_tag="PLP58_09735"
     CDS             2801..3304
                     /locus_tag="PLP58_09735"
                     /inference="COORDINATES: protein motif:HMM:NF012305.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HRJ08839.1"
                     /translation="MQPTGFQKFLSAISFGLLGQPKALPRAASPSKPTTGRGSASPSQ
                     SGGSPRPPRPKREAQTVKPEEVNTERLYIGNLSYDATESDLFELFSGVGSVRNSEVVV
                     NNRTQRSKGFAFVTMGSVEEARRAVQELGGKEFMGRPLQLSGAKPIGYGDDRGDRGDR
                     DESSEAA"
     gene            complement(3583..4701)
                     /gene="gcvT"
                     /locus_tag="PLP58_09740"
     CDS             complement(3583..4701)
                     /gene="gcvT"
                     /locus_tag="PLP58_09740"
                     /EC_number="2.1.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012419499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004047 - aminomethyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system aminomethyltransferase
                     GcvT"
                     /protein_id="HRJ08840.1"
                     /translation="MSDTAALQRSPLHEKHLALGGRLIPFAGWEMPVQYSGIVDEHKA
                     VRERAGVFDISHMGQFIVSGAAALDFLNHALTNDLAELDVGQGQYTLMLNESGGVIDD
                     LIAYRIARNEYFLVVNASKITEDRAHLAALMKQRDPVDELEMRDISAQTAGLAIQGPR
                     SRAVFERVFGVESAFPERNTVLVTLHETGFMWLCGTGYTGEEGFEFFMPAATSHEWFD
                     IILDAVRAEGGLPCGLGARDTLRLEMGYPLNGSDLAPDKTPLEAGLAFFLELEKPDFT
                     GKAALVAQKESGLPCKLAAFKMTGSCPPPRPHYPVVHEGVVVGEVASGTQSPSLGCGI
                     GMAYLPPAAALIGRHIEIEIRGRRFPAEVVKKPFFKKA"
     gene            4788..5663
                     /locus_tag="PLP58_09745"
     CDS             4788..5663
                     /locus_tag="PLP58_09745"
                     /inference="COORDINATES: protein motif:HMM:TIGR00255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YicC family protein"
                     /protein_id="HRJ08841.1"
                     /translation="MNSMTGFGRGEARQGETLWQVECGSVNRKQLEVAVNLPRDLSTL
                     EGEIRAQVAAACSRGRVHVSVKRHDAEGAANIPQIHEAAVEHYLARLFSLAGRLGISA
                     EISMSELLRLPGVLGGESEEARPEEVWPHIQGALASALEQMLRMRGTEGAHLREEIEA
                     RLKGIESLLEQIAEKAPLVPEQQRAALRQRLEQAGLPLPLDDERLVKEIALFADRTDI
                     SEELSRAASHVKQFRAYLGSGEPSGRSLDFLIQEFFREFNTMGSKCNNAAIAHCVVSA
                     KTELEKIREQIQNVE"
     gene            complement(5635..7077)
                     /locus_tag="PLP58_09750"
     CDS             complement(5635..7077)
                     /locus_tag="PLP58_09750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ08842.1"
                     /translation="MNKKQALMAALIVVLAAVLARRGPADAQFHLAVFSVDVTPPPGH
                     PGMGGGIMPARSVDDPLYAKGFVLSGGGHPWVLVSFDWCEIRGTSFDKWQSALALAAG
                     TLPERVMIHSTHVHDAPVMDEDAEKLLREMEATGAWREIPAPPPDAPVQNASVCWPDF
                     NEQCIQHVAEALRASLTKARRVTHIGMGRAEVSEVAGNRRFVRPDGTVSYARGSRTTD
                     AEARAADAGTVDPRLRTLSFWEGEQALCALHGYAVHPMSVYGQGRVSADFPGLARERM
                     QREHPDIFQIYASGCSGNVTAGKWNSGGEGSREELAAKLQDAMRRAWQDTRRAPLTRA
                     DFHQAFMPLGARNTPGHTEAVLRGHLARGERPFQRSEAAMGLAWYERVRRGHQVRVPC
                     VDLGPALVLLLPAEAYVEFQIFAQECRPESFVFTLGYGECGPGYIPIERAWEEKDSNL
                     NGWTWVPPGSEAVMRTAIRAVLTPRSESAP"
     gene            complement(7130..8389)
                     /locus_tag="PLP58_09755"
     CDS             complement(7130..8389)
                     /locus_tag="PLP58_09755"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HRJ08843.1"
                     /translation="MNQDHTHGFHILYVDDEKMALRYFRQIFEDEYIIHTADNAADGY
                     RILEAYGARIGVLLTDQRMPGESGVELMERARRLNPSIVRILVTAYTDYETAVKAVNE
                     GRAYRYLHKPIDPDELGGVLREAMAHYHEMLERERLLVEKAETIRGSLMADRVAGMGI
                     MAEGLNHHLRNALTVVRAFIDLAPIKLMEEIAGRAPRDTMFWVETQNQAQAQLERIQS
                     LLSNLAHASHARKLERDEQVRLDAVIAEQTALFDGHMHDRNVSFRFEAQPDFPPILVH
                     GERFRQLWRLIFIEEITHLKPGEGFEVSAELDTDPVNGQEQAVITLTDNGGWEANDRA
                     ANLFDPFFTRSRKPDDFGVNMMACFVTVHLHGGSIEARRLEPKGLELVMRLPLNPEES
                     TRQSEDFVQRTLENEEKWRRRTEMAAA"
     gene            complement(8386..9873)
                     /locus_tag="PLP58_09760"
     CDS             complement(8386..9873)
                     /locus_tag="PLP58_09760"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HRJ08844.1"
                     /translation="MAAISSLAPASAPLDFSVARKAAPAQAAAKPVKAEMEAVFRQAE
                     KSERFEAEIRELNLRNARLGAWFVMILVPFCSVLDWLAYPQRFWEFLVLRIACSALCV
                     PLLLALDRPWAGRYHRVYPVILPLLPALAICLMIYFTRDGASPYYAGLMLCLVGTSFV
                     FHWTFREIGLTVALVLAFYLAATLPNLSLTSDQRSMGLFINNSIFILLTCVVLYFGSR
                     QHHAIRVREFVNRCKVEAQREELSRRNEELTDTLRRLRETEAQLTQSEKLASIGRLSA
                     GIVHEINNPLNFVKSAVFLLKKKTAQMPPELAGTVTTVLNDIGEGVGRVSDIVSDLRT
                     FAHPEARAAARPVNLAAAAARAARFMAKAFGDKGARLHCAVPDDLTALGDENHLIQIL
                     INLMQNGLDAQGAGAFVEVSAAPAAGSRLELRVRDGGKGIPQEHLARVFDPFFTTKEV
                     GQGMGLGLSLCYRMMQGMGGDIRVTSRPGDTCFILNLPCAAPAQS"
     gene            10387..11079
                     /locus_tag="PLP58_09765"
     CDS             10387..11079
                     /locus_tag="PLP58_09765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009958667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HRJ08845.1"
                     /translation="MIEERYQALVAKLPPDQQAWERVLQDQLGGFYLPIHKREKIAGR
                     SSAWDFVQDDPKLPRVLLIGDSVSRGYTQAVRKALAGKVNVHRAPANCGPTASGLKNI
                     EVWLGDGKWDLIHFNFGIHDRNTPIADYQQRLEQLIERMKKTGAKLMWAGSTPCPDTK
                     DGKYKSAPIVERNTAAAEVMKKHGIAIDDLFTAITPHLATMQNPDDVHFNGQGYDFLG
                     ETVAKAIEAALK"
     gene            11094..11993
                     /locus_tag="PLP58_09770"
     CDS             11094..11993
                     /locus_tag="PLP58_09770"
                     /inference="COORDINATES: protein motif:HMM:NF012363.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase fold domain-containing
                     protein"
                     /protein_id="HRJ08846.1"
                     /translation="MNRHLLVFLLLAAPALAQTAAKKKSAMGADAPPGKVYIYKESAA
                     KPRQMEIYFPPNHDPAKSKVPGMILFHGGGWGGGSLGQFRIACAYFASRGLVCATAEY
                     QMLSKADAAKLPAGETKKRVCVTDAKSAIRWFKQHAGELGIDPQRIITGGGSAGGHIS
                     ALATMNPGLNDPADPEDIDTRVVAYVWFNPAFATDDDKDPEIDVLRHLKADLPPAIAF
                     FGDMDGWKKGWDTAHAKWKSLGTKTIDLQIAPGQSHSFFNNGPWQTVTLIAADQFLVK
                     HGLLTGEPTKTMPATGEKLVPMP"
     gene            12020..12808
                     /locus_tag="PLP58_09775"
     CDS             12020..12808
                     /locus_tag="PLP58_09775"
                     /inference="COORDINATES: protein motif:HMM:NF018178.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1080 domain-containing protein"
                     /protein_id="HRJ08847.1"
                     /translation="MRFIPFLLVAFVLSVPSLAQDGFTSLFNGRDLSGWDGDPALWKV
                     ENGIVIGTNASPEAMANNSFLIWRGGTVKNFELRATVRVIGDNNSGIQYRSREMKDVA
                     SWVITGYQCDIHPAIEHTGMTYEERGRGIFGLNGKDVMLDPEGALWQLSEHAPVKVDV
                     SQWNEYVILAQGNRLQHFINGQPTSELIDHHADKRALEGLLAIQLHKGNPNRVEIKDL
                     RLKVLPEVPLVPFEPAKLPATATKVEKPRTSRPQGTGPVVPVKK"
     gene            complement(12811..12948)
                     /locus_tag="PLP58_09780"
     CDS             complement(12811..12948)
                     /locus_tag="PLP58_09780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ08848.1"
                     /translation="MAHNRRSTKYFKVDLEKRAPNYMEVKEYVSYSAKTGETYIGFQD
                     T"
     gene            complement(13003..13320)
                     /locus_tag="PLP58_09785"
     CDS             complement(13003..13320)
                     /locus_tag="PLP58_09785"
                     /inference="COORDINATES: protein motif:HMM:NF024976.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction enzyme HsdR N-terminal
                     domain-containing protein"
                     /protein_id="HRJ08849.1"
                     /translation="MASMPDARSRTHHRPSSKRPNLEPCPRKWLLETPEEAVRQEYLC
                     ILVNDYGFTLEQIDEEVFIPGPRGNKNARADFVIWRTVQERRDGKTALIVVECKADNV
                     AID"
     gene            13570..15150
                     /locus_tag="PLP58_09790"
     CDS             13570..15150
                     /locus_tag="PLP58_09790"
                     /inference="COORDINATES: protein motif:HMM:NF020976.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase D family protein"
                     /protein_id="HRJ08850.1"
                     /translation="MPPPLYPLTTPQTLNRRTFLAGSASLALATLTRGHAYGAVARAP
                     KFSAYPFSLGVASGDPAPDGFVLWTRLAPQPLLGDGGMAPEAVEVSWQVCEDEAMTKI
                     AAKGTAVATPEWGHSVHVEVEGLRPDRWYWYQFKAGSETSPTGRSRTFPAADAIPERL
                     RFAFASCQHYETGLYTAYEHMLAEAPDLVIHLGDYIYEYAGIENRVRKHVGPEITTLH
                     DYRVRLAQYKSDPALQAMHAAAPWLVTWDDHEFDNNCAGDISEEPGVSREDYLKRRAA
                     SYQAYYENMPLRRAALPKGPDMLLYRRVPCGRLADFHVLDTRQYRTDQPCGDGNKPPC
                     PESLSPDATLLGHTQRDWLFENLAKSPAQWNVLAQQVMMARVDRAAGPEVTHSMDQWP
                     GYEMERRRVLKYLHEQKIKNPVVLTGDIHSNWANNLIADFDDLDSRVVATEFVGTSIS
                     SGGDGVLKPKTLAETYAQNPFVKFHSTERGYVSCEITPKLWHTKYQGVEYVSKPGAPL
                     RTRASFVVEAGNPRLQKA"
     gene            complement(15175..16590)
                     /locus_tag="PLP58_09795"
     CDS             complement(15175..16590)
                     /locus_tag="PLP58_09795"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase"
                     /protein_id="HRJ08851.1"
                     /translation="MIRTLLAFALLCAPLLPAQERAPNVILMLVDDLGWTDLGCQGSK
                     YYETPNIDQLARDGMRFTDAYSACTVCSPTRAAVLTGKAPARLHITDWIAGHQRPYTR
                     LKSPEWTKHLPLEETTLAEHLREAGYATASIGKWHLGGAEFGPEKHGFDVNIAGYEAG
                     SPPSYFSPYKIPTLTDGPEGEFLTDRLTSEAVQFIQRNKERPFLIYLPHYAVHTPIMG
                     KPEVAAKYRAKGPAGNHHDPVYAALVESVDDSVGRLRAALKELSLDENTIFIFTSDNG
                     GLSHLVNAQGWRRGPTDNSPLRLGKGGPYEGGVRVPLIINWPAVVEAGGVNDTPVISH
                     DLAPTVLEAAGVTPGAEHVMDGVSLLPLLTQKDFLKRDAIYWHYPHYHPGSAAPHSAI
                     REGDWKLVFFHEDKRAELYNLRRDPGEMDNVADIEGDVAGRLVQALDGWLESTGAQMP
                     EDNPDFNIERAWESPRPAKKK"
     gene            complement(16615..17547)
                     /gene="trxB"
                     /locus_tag="PLP58_09800"
     CDS             complement(16615..17547)
                     /gene="trxB"
                     /locus_tag="PLP58_09800"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007414842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004791 - thioredoxin-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HRJ08852.1"
                     /translation="MENVIIIGTGCAGYTAAIYTGRANLKPLILSGSMPGGQLTTTTE
                     VENFPGFPNGIMGPELMMNMQTQAEKFGARIEYATVESVAKTADGHFDIKTEDGTVRQ
                     ARAVIIATGASPKHLGLPNEKGLIGHGLTSCATCDGAFYRNVPVCVIGGGDSACEEAM
                     FLTKFASKVYLIHRRDTLRASKIMADRALANPKIEPVWNSAVTEYLTDDKGEMRAVAL
                     EDLVTGEKSELELRCVFVAIGHVPNAAFLGGLVDRDENGYILQTRGTRTKTEGLFAAG
                     DVADHVYRQAITAAGQGCAAALEAERYLAEQGVH"
     gene            complement(17665..18105)
                     /locus_tag="PLP58_09805"
     CDS             complement(17665..18105)
                     /locus_tag="PLP58_09805"
                     /inference="COORDINATES: protein motif:HMM:NF017774.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HRJ08853.1"
                     /translation="MDGGPSHYLIVDGHSVMHAWPELRSAMRNSARRNMARLELMRVL
                     RVYQDMSGAQVVLVFDGTHQARAEEREPGGLQVIYAEAGTTADAIIERLASKYAPVHP
                     LRVVSADGMVRETVLASGADWTSPEMLRRLCEDAEEKLRGMIGG"
     gene            18205..19329
                     /locus_tag="PLP58_09810"
     CDS             18205..19329
                     /locus_tag="PLP58_09810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007417742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HRJ08854.1"
                     /translation="MPAKKINIAVVGLGFGAEFIPIWQRHPHTACHAVCQRDPKKLKA
                     VGEAFGVGKRFTDFHEMLNDPEIDAVHINSPIPDHAWMSIAALKAGKHVACTVPMATS
                     IEDCRTICELVRKTGLKYMMMETVVYAREFLFMREQLHKGKLGKLQFLQASHQQDMDG
                     WPNYWPGLPPMWYATHCVGPVAALAGAPAEYVSCFGSGTIRPELIKCYGSPFAVETAH
                     VKFKGTDLSARVIRSLFDTARQYRESIDVYGDKASIEWPLIEHEPLVMHTAKLPEHKI
                     PKHVKAPDYAKLLPKSIRDFTTKGVYDLAKKTHLSFTQGSGHGGSHPHLAHEFAMALV
                     EDRDPFPNAKQSANWTCVGLCAHESALAGGKIVKLPAFTQ"
     gene            19392..20321
                     /locus_tag="PLP58_09815"
     CDS             19392..20321
                     /locus_tag="PLP58_09815"
                     /inference="COORDINATES: protein motif:HMM:NF012958.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase-fold protein"
                     /protein_id="HRJ08855.1"
                     /translation="MSRLLPCLILASISFANGQESKKARAAVQPFSWVSPMQNAEWAR
                     KSLPATVRHTTFKSASMGIEVGCYIYLPPGYESSQERYPVVYHLHGGRPGAENKAVRL
                     ASHVDAAIQKGTIKPTIYVFPNGGPVSWYDMPEIPNGLGETVFVKELVPHIDATCRTW
                     GTREGRALEGYSQGGRGTTRIMFKHPELFLSVAPGGSGYGPEKHIQENDGYENDTLRF
                     LPVGYNAWDLAKAYAARADKPALSILVWDGTKCFNYDFNLQYSAYLKELGIAHEFLSI
                     PDVPHTATGSYDVKGDEIMRHHQKAFDAHRPAR"
     gene            20356..>21635
                     /locus_tag="PLP58_09820"
     CDS             20356..>21635
                     /locus_tag="PLP58_09820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ08856.1"
                     /translation="MTDKLTRRHSLKLLTAGSLTAGLSSRHSLPAQTPDAKPARDPWA
                     KTHDRVFLGGEFWANPMEDWRVSEGGAECLNAGPGRSVHSLTHQITRNGSFTMAVTLT
                     RLEASANDGGAGFRFSLRSEIDEHRSNCFVQRGLDAGWQGEKLILGPKTAPLPGGAAL
                     PKLRLTLAGAPQGGQCALTLTAADAATGAPLATLTHTAPAQAVLGNVSLANSYAPGGA
                     GAAKGKKGKAGAVTGGRYRFQDWTFEGDAFTVTESQRFGPILWSMYSLSDSRGPEAFV
                     MKMSALTGPLGARDNHEVELHIQKGGAWQSLGTAKLDTDAWTATFRIPNWDEKQATPY
                     KLVYQEKHTDGTTTAHEWTGTIKANPEGRPLRMAALTCQNDYGFPYAPVADNVRKLDP
                     DLVFFSGDQLYENHGGFGIIRDDAGRAILNYLRK"
BASE COUNT         3941 a         7103 c         6839 g         3752 t
ORIGIN      
        1 catgaggggc tggagtttgc cgtcctcata gacgggggcg gtgccgccgg gggcgctgat
       61 gacgcggtcg tcggcgccga ggatgacggt gaagccggag gggttctgcg ggagtttgtt
      121 gatctcgggg gtcttgctcc agcagacgcc ggcatggatt tcctgtccgg cgatgacggg
      181 gcggcagacc atggcgccgg gcacgggaat ggtgcccaga tattggccgt cgaggctgaa
      241 gcggcggaag cagttgtcgg cgcgggaggt gacgatcacg gtggctttgt cagcgccctg
      301 gcgggtgtca atggcgatgc cgtgggcgtt gttcagcctc tgttcggcgg gcacgccgtc
      361 cttgccgccg aagcgctgga ggaacttgcc gcgcgcgtcg taacgcagca cgaactggga
      421 gccgtagccg tccaccacat aaatgtcgcc attcggggcg atggcgactt cggtggggtt
      481 gaagaacatt tccggctcgt acgcgccgac gctcatgggg tggctgatgg agaagatttc
      541 ccttccggcg aggtcaatct ttgccacgcg cccggtctgg cggtagccgc cggagccgga
      601 cttccacagg ccgctgtcgg tgacgaagag gaactcctcg ccgttttcct cggagagggt
      661 gaggccgtgg ccgccgggcc agacggagcc ccaggagtcg aggatggtgc cgtcgggttt
      721 gaagacgagc atctggttgt cccaatgatc tcctaccatg aagagacggc cgtctttgac
      781 ctggaccatc tcgtggcagt tgaggatggg gtggtacctg ccctggccgg gggcgaccca
      841 gtccttgacg accttgtaac ggtggctgcc gtggccgatg actacgtcct ttttcgggtc
      901 gggcgcggcg cgcagggtct gcgtccaggt ggcggcggcg gcactgccga ggccggcgag
      961 gaaatgacgg cgggtcttca tggcggaaaa gggcggggtg gcaggcgcgg ggcggtcaat
     1021 gcgtgacttc ttccggcggg gcgagctgct tgggcgggaa gaaggcgatg agcaggagca
     1081 cggcggcggc gagggagagg ccggtgggcc agaggaaaag ctccttgtag ttcacgacgc
     1141 cgtccacagt gagtttgccc tggaggggaa tgaagaacca tttggcggcg aggtcaccga
     1201 ggccgagaac gaggaggttg aagaggccct gagcactgga gcgcgcgtcc ttcgggaagg
     1261 cggcgtcaat gaagatgtag agcgtggcga agaagaaggc gtagcagatt ccgtgcagga
     1321 cctggacggc gataatcatg gtctgattct ccggcatgaa ggcgaagacg gcaaagcgcg
     1381 cggcatggcc gaggatgccg aggatcatgg tggtcttcca gccgaggcgg gaaaggaacc
     1441 agccgagcac ggccatggtg aggatttcgg cgatctggcc gatgctcatg acgggcatga
     1501 tccatttttg ctcaatgcca accacgtcgc tgccgaggaa gttcccggcg aggagaaagt
     1561 agccgttgtg aatggtggcg tctatgaagg tgacgatgaa gagaatcagc agaaagggct
     1621 ttttgaggaa catggcggcc ttcagccagg cgaagttgct ctcgcccttc gaggcgggtt
     1681 tggcgggggt gtgcggcagg ctgaggctga acagggagag ggcgatggag gcggcgccgg
     1741 agatgaggaa gatgttggcg cgggcggcga tggcgtcggc gccggtcttg ccctccagga
     1801 tgaaatagag cggccaggcg gcgaggatcc agccgatggt gccgcccatg cgcacgaggc
     1861 cgaactcctt ctgcgcgtcc ttcaagtggg tgaaggcgat ggagtttgag acggagatgg
     1921 tcggcacata aagcagcgag tggacgagca tcaggccgaa gaaggtgggg aaatcctttg
     1981 cccaatacat gccgaggatg gccaggccgc cgacgaggtg gctgaaggac atgaattttt
     2041 ccgcggcgaa gtggcggtcg gcgaactggt tgctgaagaa gatgccgagc acggaggcga
     2101 tggcgaaggc gctgccgacc caggtgatct gcaagtcggt gaacttcagt ccgtccgctc
     2161 ccatgtagcc ccagatgagc ggcagccagg cgccccagat gaagaattcc aggaccatca
     2221 tgacgaagag ctttttggcg gcgggtgatt tcataattaa agcgcgcgag agtaggaagc
     2281 cgccgccggg aaggcaagaa gggattgcgc ccggaatcca aggttgaccg ccatgcaggg
     2341 ttcctttagt ctcggcgcgc agtcgtttgg agcgctcgtg ctccgatccc tccccaccca
     2401 gcctttgcgc catgagccac gctttgaccg tgccagatgc catgaatgga cccgcgattc
     2461 catcgcgggg agggttcgcc gtgattgaac gtcacacgca cactggaccg gccgtatggc
     2521 ggtccacgcc gcccgactgc ggcaccggac gggatggatg cgggccgcgt gtgcggtccg
     2581 gcttggttcc cggtccgctg gagatgacgc ttcccgcgtc cccgccgccg gccattttcc
     2641 atgtccgtca gaacggtgcc tgcaaccctg aaacaccacc cctgaacgaa cacccatgtc
     2701 agactcgaac tcgcaagaaa accgccgccg ccgcaatcgc ggcccccgcg gtccccgcag
     2761 tggcccgccc cgtccgggca aaaccccgcg cgcaggagct gtgcagccca ccggcttcca
     2821 gaaattcctg agcgccatca gctttggcct gctcggccag ccgaaagccc tgccgcgcgc
     2881 cgcctccccc tccaaaccca ccacaggtcg cggttccgcc tccccttcac aaagtggcgg
     2941 ctctccacgt ccgccacgcc caaaacgcga agcgcagact gtgaaaccag aggaggtcaa
     3001 caccgagcga ctctacatcg gcaacctttc ctatgatgcg acggagagcg atcttttcga
     3061 gcttttcagc ggtgtcggca gcgtgcgcaa ctcggaggtg gtggtcaaca accgcaccca
     3121 gcgctccaag ggtttcgcct ttgtgaccat gggttcggtg gaggaggccc gccgcgccgt
     3181 gcaggaactc ggcggcaagg agttcatggg ccgccccctc cagctcagtg gcgccaaacc
     3241 catcggttac ggcgatgatc gcggagaccg gggcgaccgc gatgagtctt cggaggccgc
     3301 gtaagtctgc ccgtctttca cccagcccgc aggccgcatg gtctgcgggc ttttttgtga
     3361 tcgagagcgc agtccatgcc ttcgcaggcg cggctccttg cggccgctgc ctcaaggcgg
     3421 tggagagggt gtgtttctga ggcgcacttc gcgctttcgc cagaatgggg caaccttcca
     3481 gattgcccgc gcctgcggag tgaccgccca ggcactgatg cgccccgcat tctcacgccc
     3541 caggtcggtt gcacaaaccg cccgatccta ggacaagcgc gcttacgctt tcttgaaaaa
     3601 cggctttttg accacttcgg cgggaaagcg gcggcctcgg atttcgatct cgatgtgccg
     3661 gccgatcaat gcggcggcgg gcggcaggta ggccatgccg atgccgcaac cgaggctggg
     3721 cgactgcgtg ccgctggcca cctcgcccac gaccacacct tcgtggacca ctggataatg
     3781 cgggcgcggc ggggggcagg agccggtcat tttgaaggcg gcgagtttgc agggaagacc
     3841 gctttccttt tgcgccacga gcgcggcttt gccggtgaag tccggctttt cgagttcgag
     3901 aaaaaaggcc agcccggctt cgagcggcgt cttgtccggc gcgaggtcgc tgccattgag
     3961 cgggtagccc atctccagcc gcagcgtgtc gcgcgcgccg agtccgcagg gcaggccgcc
     4021 ctcggcgcgc acggcgtcga ggatgatgtc aaaccactcg tggctcgtcg cggcgggcat
     4081 gaaaaactca aaaccctcct cgccggtgta gccggtgccg cacagccaca tgaaacccgt
     4141 ctcatgcagg gtcacgagca cggtgttgcg ctccggaaaa gcgctctcca cgccgaacac
     4201 ccgctcgaaa acggcgcggc tgcgcggccc ctggatggcg agtccggcgg tctgcgcact
     4261 gatgtcacgc atctccagtt cgtccacggg gtcgcgctgc ttcatcaacg cggcgagatg
     4321 cgcccggtcc tcggtgatct tcgaggcgtt gacgacgagg aagtactcgt tgcgcgcgat
     4381 gcggtaggcg atgaggtcgt caatgacacc gccgctttca ttgagcatga gcgtgtactg
     4441 cccctgcccg acatccagtt cggcgaggtc gttggtcagg gcatggttga ggaaatccag
     4501 ggccgccgcg ccgctgacga tgaactggcc catgtgcgag atgtcgaaga cgcccgcgcg
     4561 ctcccgcacc gccttgtgct cgtccacaat gccgctgtat tgcacgggca tctcccagcc
     4621 cgcgaacggg atcagccggc ctcccagggc gaggtgtttt tcatgcaggg gagagcgttg
     4681 gagagcggcg gtgtcggaca tgtcgcgcca aggtggcggg aggcgcggct ttgtcaaagc
     4741 acgcgacggc tgttgcgccc gcgcccggcg cctgctttga tgcgccgatg aatagcatga
     4801 ccggatttgg gcgcggcgag gcgcggcagg gcgaaaccct gtggcaggtg gagtgcggca
     4861 gtgtgaaccg caagcagctc gaggtggcgg tgaacctgcc gcgcgacctt tccaccctgg
     4921 aaggcgagat ccgcgcgcaa gtggccgccg cctgctcgcg cggccgcgtg catgtgagtg
     4981 tgaagcggca cgacgcggaa ggcgcggcga acatcccgca aattcacgag gcagccgtgg
     5041 agcactatct ggcgcgcctt ttctccctgg cgggccggct ggggatctcg gcggaaattt
     5101 ccatgagcga gctgctgcgc ctgcccggcg tgctcggcgg cgagagcgag gaggccaggc
     5161 cggaggaagt gtggccgcac atccagggcg ctctggcctc cgcgctggag cagatgctgc
     5221 gcatgcgcgg cacggagggg gcgcatctga gggaggaaat cgaggcgcgc ctcaaaggca
     5281 tcgagtccct cctggagcag atcgcggaaa aagccccgct ggtgccggag cagcagcgcg
     5341 cggccctgcg ccagcgtctt gagcaggcgg gcctgccttt gcctctggat gacgagcggc
     5401 tggtcaagga gatcgccctg ttcgccgacc gcaccgacat ctccgaggag ctttcccgcg
     5461 cggccagcca tgtgaagcag ttccgcgcct acctcggctc gggcgagcct tccgggcgca
     5521 gtctggactt cctcattcag gagttcttcc gcgagttcaa cacgatgggt tccaaatgca
     5581 acaacgccgc catcgcccac tgcgtggtca gcgccaagac ggagctggaa aagatcaggg
     5641 agcagattca gaacgtggag tgagcactgc gcggatggcg gtcctcatca ccgcctcgct
     5701 gccgggcggc acccaagtcc agccgttgag attgctgtcc ttctcctccc aggcgcgctc
     5761 tatggggatg tagcccggcc cgcattcgcc atagcccaga gtgaaaacaa aactctccgg
     5821 cctgcattcc tgcgcgaaga tttggaactc cacataagcc tccgcaggca gcagcagcac
     5881 cagcgccggg ccgaggtcca cacaaggcac gcgcacttgg tgcccgcgcc gcacgcgctc
     5941 ataccaggca agtcccatcg cggcctcgga gcgctggaag ggccgctcac cccgcgccaa
     6001 atgaccgcgc agaacggcct ccgtgtgccc tggagtgttg cgcgcgccca gcggcatgaa
     6061 cgcctggtgg aagtccgccc gtgtcaaagg cgcgcgccgc gtgtcctgcc aggcgcggcg
     6121 catggcgtct tgcagcttgg cagcgagctc ctcgcggctg ccctcgccgc cgctgttcca
     6181 tttgccggcg gtgacattgc cggagcagcc gctggcgtag atttgaaata tgtccggatg
     6241 ctcacgctgc atcctctccc gcgccaggcc gggaaaatcg gcggacaccc tgccctgccc
     6301 gtacacgctc atcggatgca cggcataacc atgcagcgcg cacagggcct gctccccctc
     6361 ccaaaaactc agcgtgcgca gcctggggtc caccgtgccc gcgtccgctg ccctggcctc
     6421 cgcgtccgtg gtgcggctgc cgcgcgcgta actgaccgtg ccgtccgggc ggacaaagcg
     6481 gcggttgcct gccacctcgc tcacctcggc cctgcccatg ccgatgtggg tgacgcgcct
     6541 ggctttggtc aatgatgccc gcagcgcctc cgccacatgc tggatgcact gctcattgaa
     6601 gtccggccag cacacggacg cgttctgcac gggcgcgtcc ggcggcggcg ccggaatttc
     6661 cctccatgcg cctgtggcct ccatctcgcg cagcagcttc tccgcgtcct catccatcac
     6721 cggcgcgtcg tggacatgag tcgagtgtat catcacccgc tccggcagcg tgcccgccgc
     6781 caatgccagg gcgctctgcc atttgtcaaa cgaagtgccg cgtatttcac accagtcaaa
     6841 actcacgagc acccacggat gtccgccgcc gctcaaaacg aagcctttgg cataaagggg
     6901 atcatccact gagcgcgccg gcatgatgcc gccgcccatg cccggatgcc ccggcggcgg
     6961 cgtcacatcc acactgaaca cggccagatg gaactgcgcg tccgccggtc cgcggcgcgc
     7021 gagcaccgcc gccagcacga cgatcagcgc ggccatgagg gcttgttttt tgttcatgcc
     7081 acactcatac gcggcatgag gtcgcaaacg tgcccgccgg cctgtcaatt caagcggccg
     7141 ccatctccgt gcgccgccgc catttctcct cgttttcgag ggtgcgctgg acgaagtcct
     7201 cggactggcg ggtggattcc tcaggattga ggggcaggcg catgacgagt tccagcccct
     7261 ttggctccag ccggcgcgcc tcgatgctgc cgccgtggag atgcacggtg acaaagcagg
     7321 ccatcatgtt caccccgaag tcgtcaggct tgcggctgcg ggtgaagaag gggtcgaaaa
     7381 gattggccgc gcggtcgttg gcctcccagc cgccattgtc ggtgagggtg atgaccgcct
     7441 gctcctgtcc gttcacagga tcggtgtcga gttcggcgct tacttcgaat ccttcccccg
     7501 gcttcaaatg cgtgatctcc tcgatgaaga tcaggcgcca gagctgccgg aagcgctcgc
     7561 catgaaccag gatgggcggg aagtccggct gagcctcaaa tcggaagctc acgttgcggt
     7621 cgtgcatgtg gccgtcgaaa agcgcggtct gctcggcgat gacggcatca agccggacct
     7681 gctcgtcgcg ctccagtttg cgcgcgtggg aggcgtgagc caggtttgaa aggagggact
     7741 ggatgcgttc gagctgcgcc tgcgcctggt tttgcgtctc gacccagaac atcgtgtcgc
     7801 gcggcgcgcg cccggcgatc tcctccatca gcttgatggg cgccaggtca atgaaggcgc
     7861 gcaccaccgt cagtgcgttg cgcagatgat ggttgagacc ctccgccatg atgcccatgc
     7921 cggcaacgcg gtcggccatc aggctgccgc ggatggtctc ggctttttcc accagcaggc
     7981 gctcgcgctc cagcatctcg tggtaatgcg ccatcgcctc gcgcagcacg ccgccaagtt
     8041 catccgggtc tatgggcttg tgcaggtagc ggtaggcgcg gccctcgttg acggccttca
     8101 ccgccgtctc gtagtccgtg taggccgtga ccaggatgcg cacgatgctc ggattcagcc
     8161 gccgcgcgcg ctccatgagc tccacgccgc tctcgccggg catgcgctgg tccgtcagca
     8221 gcacgccgat gcgcgcgccg tacgcctcca ggatgcggta gccgtcggcg gcgttgtcgg
     8281 cggtgtgaat gatgtattcg tcctcgaaaa tctggcggaa gtaacgcagc gccatttttt
     8341 catcgtccac atagaggatg tggaatccgt gggtgtgatc ctggttcatg actgggcggg
     8401 ggcggcacag ggaaggttaa ggatgaagca ggtgtctcca gggcggctcg tcacgcggat
     8461 gtcgccgccc atgccctgca tcatgcggta gcagaggctc aggccgaggc ccatgccctg
     8521 gcccacctcc ttggtggtga aaaaaggatc gaacacgcgc gccagatgct cctgggggat
     8581 gcctttgccg ccgtcgcgca cgcgcagctc cagcctgctt cccgccgccg gcgcggcgct
     8641 gacctccaca aacgcccccg ccccctgggc gtcgaggccg ttttgcatca ggttgatcag
     8701 gatttgaatc agatggttct catcaccaag agccgtcagg tcatccggca ccgcgcaatg
     8761 cagccgggcg cccttgtcgc cgaaggcctt cgccatgaag cgcgccgccc tcgcggcggc
     8821 ggcggccaga ttcaccggcc tcgccgcagc gcgcgcctcg gggtgggcga aggtgcgcag
     8881 gtccgacacg atgtccgaca cccggcccac tccctcgcca atgtcgttga gcaccgtcgt
     8941 caccgtccct gccagctccg gcggcatttg cgccgtcttc ttcttcagca gaaaaacagc
     9001 ggacttgacg aaattcagcg ggttgttgat ctcgtgaacg atgcccgcgc tcagccgccc
     9061 gatcgacgcc agcttctcgc tctgagtgag ctgggcctcc gtctcccgca ggcgccgcag
     9121 cgtgtccgtc agctcctcgt tgcgccggct cagctcctcg cgctgcgcct ccaccttgca
     9181 gcggttcaca aactcgcgca cgcggatggc atggtgctgg cggctgccaa agtagagcac
     9241 cacgcaggtc aggaggatga aaatactgtt gttgatgaac aaacccatgg agcgctgatc
     9301 gcttgtgagg gagagattcg gaagggttgc ggccaggtaa aaggcgagca ccaatgccac
     9361 cgtcagaccg atctcccgga acgtccagtg gaagacaaac gacgtgccca caaggcacag
     9421 catcagcccc gcgtaatagg gggaggcgcc atcccgcgtg aaataaatca tcaggcagat
     9481 cgccagcgcg ggcagcagcg gcagtatcac cggatacacc ctgtggtagc gccccgccca
     9541 aggccggtcc agggccagca gcagcggcac gcacaacgcc gagcaggcaa tgcgcaggac
     9601 cagaaactcc caaaatctct gcggatacgc cagccagtcg agcaccgagc aaaacggcac
     9661 cagaatcatc acaaaccacg cccccagcct ggcattgcgc aggttcagct cgcggatttc
     9721 cgcctcgaaa cgttcggatt tctccgcctg gcgaaagacc gcctccatct ccgccttcac
     9781 cggcttcgcg gctgcttgtg caggcgcggc cttgcgcgcc actgagaaat ccagtggtgc
     9841 cgaagctggc gcgagcgagg aaatagcagc cataatttat ataacagtca taaacaatag
     9901 acctctctta agccttcgca acccgcgatt cggcggaaaa ttggaaaaat cgggctgccg
     9961 ggatggatga ccgtattcgc acttgctcaa cactcgtcct ctgctccgcg ccagcgcagg
    10021 gacactcccg tgccgcaagt cgctttcagt ttgccggttc gttttgatga cagggcgttc
    10081 cttgctggaa atttcctctc tggatcatcc ttcattatga gtgtcatatt cgacaaaccc
    10141 acgatcagtg cctttgatga tctcgacgtc cgcatgaaac atggcctgtg ctgatgccgg
    10201 agacggcggc gtgaatatgg aggcgtgggc gggcgtttag agagacccat gttccgtttt
    10261 ttcagctcag ttattttaat ctcctgcctc tcttccctgt ctttggccca gaccccgacg
    10321 aatccgccca aaggagagcc caccaacgcc gcggaggccg ctgcgcagaa ggccaaagca
    10381 gatgccgtga tcgaagaacg ctatcaggcg ctcgtggcga aattgccgcc ggaccagcag
    10441 gcctgggaac gcgtgctgca agaccagctc ggcggatttt acctgccaat ccacaagcgc
    10501 gagaagatcg ccggacgctc cagcgcgtgg gatttcgtgc aggatgatcc gaagctgccg
    10561 cgcgtgctgc tgatcggcga ttcggtttca cgcggctaca cgcaggcggt ccgaaaggcg
    10621 ctcgctggca aggtgaacgt ccatcgcgcc cccgcgaact gcggccccac cgccagcggc
    10681 ctcaaaaaca tcgaggtctg gctcggcgac ggcaaatggg acctcatcca tttcaatttc
    10741 ggcatccacg accgcaacac gcccatcgcc gactaccagc agcggctgga gcagctcatt
    10801 gagcggatga agaagaccgg cgcgaagctc atgtgggccg gcagcacacc ctgcccggac
    10861 acgaaagacg gcaaatacaa gtccgcgccc atcgtggaac gcaacaccgc cgctgcggag
    10921 gtgatgaaga agcacggcat cgccatcgac gaccttttca ccgccatcac gcctcacctg
    10981 gccacgatgc agaatcccga cgacgtccac ttcaatggcc agggctatga tttcctcggc
    11041 gaaaccgtcg caaaggccat cgaggccgct ctgaagtaaa cctgatccat cgtatgaatc
    11101 gccaccttct tgtctttctg cttctagccg ctccagcgct cgcccaaacc gccgcgaaaa
    11161 agaaatccgc gatgggggcg gatgcaccac ccggcaaagt ttacatctac aaggagagcg
    11221 ccgccaaacc gcgccagatg gagatctact ttccgccgaa tcatgatccc gcgaagtcca
    11281 aggtgcccgg catgatcctt tttcacggcg gtgggtgggg tggtggctcg ctgggccagt
    11341 ttcgcatcgc ctgcgcatac tttgccagtc gcgggctcgt ctgcgccacg gcggagtatc
    11401 aaatgctcag caaggcggat gccgcgaagc tgcccgcagg cgaaacgaag aagcgcgtgt
    11461 gcgtcaccga cgcgaaaagc gccatccgct ggttcaaaca gcacgcgggc gagctcggca
    11521 tcgatccgca gcgcatcatc accggtggcg gatcggctgg cgggcatatc agcgcgctcg
    11581 ccacgatgaa tcccggcctg aacgaccccg cggacccgga ggacatcgac acccgcgtcg
    11641 tcgcctatgt gtggttcaac cccgccttcg ccaccgatga cgacaaagac cccgaaatcg
    11701 acgtgctgcg ccatttgaag gccgacctgc ctcccgcgat cgccttcttc ggcgacatgg
    11761 atggatggaa gaaaggctgg gacaccgccc acgcgaaatg gaagtccctc ggcacgaaaa
    11821 ccatcgatct ccagatcgcg cccggccagt cgcacagctt ctttaacaac ggcccctggc
    11881 aaaccgtcac gctcatcgcc gcagaccagt tcctcgtgaa gcacggcctc ctcaccggcg
    11941 aaccgaccaa gacgatgcct gccactggcg agaagctcgt gcccatgcct tgaccaacac
    12001 tccaagactc catcactcca tgcgcttcat tcccttcctt ctcgtcgctt tcgtcctttc
    12061 agtcccttcg ctcgcacaag acggcttcac ttccttgttc aacggccgcg acctctccgg
    12121 ctgggacggc gatccagcgc tctggaaggt tgaaaacggc atcgtcatcg gcacgaatgc
    12181 gtcgccggag gcgatggcga acaacagctt cctcatctgg cgcggcggca cggtgaagaa
    12241 cttcgaactg cgtgccaccg tccgcgtcat cggcgacaac aacagcggca tccagtatcg
    12301 cagccgcgag atgaaggacg tcgcgtcgtg ggtgatcact ggttaccagt gcgacatcca
    12361 tcccgccatc gagcacacgg gcatgacgta tgaggagcgc ggacgcggca tcttcggcct
    12421 gaacggcaag gatgtgatgc tcgaccccga gggcgcgctc tggcagctca gcgagcacgc
    12481 gccggtgaag gtcgatgtga gccagtggaa cgagtatgtc atcctcgcgc aggggaaccg
    12541 cctccagcac ttcatcaacg gccagcccac ctccgagctg atcgaccacc acgcggacaa
    12601 acgcgcgctg gagggcctgc tcgccatcca gctccacaaa ggcaacccca accgcgtcga
    12661 gatcaaagac ctccgcctca aagtcctgcc cgaagtgccg ctcgtcccct tcgagcccgc
    12721 caaactgccc gccacggcga cgaaggtcga aaaaccgcgc acgagccgcc cgcaaggcac
    12781 ggggccggtg gtgccggtga agaagtgaga tcatgtgtcc tgaaagccga tgtaggtctc
    12841 gccagttttc gctgaatagg agacgtattc cttcacctcc atgtagttcg gtgcccgttt
    12901 ttcgagatcg accttgaagt atttggtgct gcggcggttg tgggccacga agaatttggc
    12961 gcgttcgttg ttcgcgtagt tcgccccctg ctcgtaggtc ttttagtcga tggcgacgtt
    13021 gtccgccttg cactccacga cgatgagcgc ggtcttgcca tcccgccgct cctgcacggt
    13081 gcgccagatg acgaaatcgg cgcgggcgtt tttattgcct ctcgggcctg ggatgaacac
    13141 ctcctcgtct atctgctcca aggtgaagcc gtagtcattc accaggatgc agagatattc
    13201 ctggcggacg gcctcctcgg gcgtctccag cagccacttg cgcggacatg gctccagatt
    13261 tggccttttt gacgacggac ggtgatgggt tcggctgcgg gcatccggca tactagccat
    13321 gccgcggcag cgcggtcaag gagcggcgga ttgtcatacg gatgaaacac atcatgcatc
    13381 aagatggatc tgcggatgcg ggaggtggga tcgaaatcca ggcgataccc ggcgccgtcc
    13441 acgccatcgg ctccgttgat ggcgatcttt ggaggggaga gttttgacgg catgggcggc
    13501 ctggcagcgt gtgtggaaac ccggttttgc tgaaagtttg gctggcagca atgccttctc
    13561 tcatctgcca tgcccccgcc actttacccg ctcaccaccc cgcagacact gaaccgccgc
    13621 acgtttcttg ccggctccgc ctcactggcg ctcgccacgc tcacacgcgg tcatgcttat
    13681 ggagccgtgg cgcgcgcgcc gaaattctcc gcctacccgt tcagcctcgg tgtcgcctcg
    13741 ggtgatcccg ctccggatgg ttttgtgctc tggacccggc tcgcaccaca gcctctgctc
    13801 ggagacggcg gcatggctcc ggaggcggtg gaggtttcct ggcaggtctg cgaggatgag
    13861 gcgatgacga aaatcgccgc caagggcacg gcggtggcca cacctgagtg ggggcactcc
    13921 gtgcatgtcg aggtggaggg gctgcgccct gaccgctggt attggtatca attcaaagcc
    13981 ggctcggaga ccagccccac gggccgctcg cgcacttttc cggcggcgga cgccattccg
    14041 gagcggctgc gttttgcctt cgcctcctgc cagcactacg aaaccgggct ttacaccgct
    14101 tacgagcaca tgctggccga ggcgcccgac ctcgtcattc atctgggcga ctacatttat
    14161 gagtatgcgg gcattgagaa ccgcgtgcgc aagcatgtcg ggccggagat caccaccctc
    14221 cacgattacc gggtgcgcct ggcgcaatac aagtccgacc cggcccttca ggccatgcac
    14281 gccgccgcgc cctggctggt gacgtgggac gaccatgagt tcgacaacaa ctgcgcggga
    14341 gacatctccg aggagccggg ggtgagccgc gaggactatc tcaagcgccg cgccgcatcc
    14401 taccaggcct actatgaaaa catgcccctg cgccgggccg cacttccgaa aggaccggac
    14461 atgctgcttt accgacgcgt tccctgcggc cggctggcgg acttccatgt gctcgacacc
    14521 cgccagtacc gcaccgacca gccctgtggc gacggcaaca agcccccgtg ccccgagtcc
    14581 ctctccccgg atgccaccct gctcggccac acccagcgcg actggctttt tgaaaacctg
    14641 gccaaatccc ccgcgcaatg gaacgtgctg gcgcagcagg tcatgatggc ccgtgtggac
    14701 cgcgccgccg ggccggaggt cacgcacagc atggaccagt ggcccggcta tgagatggag
    14761 cgccgccgcg tgctcaaata cctgcacgag caaaagatca aaaatcctgt cgtgctcacc
    14821 ggcgacattc acagcaactg ggcgaacaac ctcatcgccg atttcgacga cctcgactcc
    14881 agagtggtgg ccaccgagtt cgtcggcacc tccatctcat ccggtggcga cggcgttttg
    14941 aagccgaaga cgctggccga aacctacgcg cagaatcctt ttgtgaagtt ccacagcacc
    15001 gagcgcggct atgtgagctg cgagatcacg cccaagctct ggcacaccaa ataccagggc
    15061 gtcgaatacg tcagcaagcc cggagcgccg ctgcgcacac gcgcgtcatt tgtggtcgag
    15121 gcggggaatc caaggctgca aaaggcgtga cgcgggccgg catcccgccg cgcgtcattt
    15181 cttcttcgca ggacgcggcg actcccacgc gcgctcgatg ttgaagtcgg ggttgtcctc
    15241 cggcatctgg gcgcccgtgc tttcgagcca tccgtcaagg gcctggacga gcctcccggc
    15301 cacatctccc tcgatgtcag ccacgttgtc catctccccc ggatcgcggc gcaggttgta
    15361 aagttcggcg cgcttgtctt cgtgaaagaa gacgagcttc cagtcgccct cgcgaatggc
    15421 gctgtgggga gcggcgctgc ctgggtggta atgcgggtag tgccagtaaa tggcgtcacg
    15481 cttcaagaaa tccttttgtg tgagcaaggg cagcaatgaa actccgtcca tgacatgctc
    15541 cgcaccggga gtcacgcccg cggcttcgag gacggtgggc gcgaggtcat ggctgatgac
    15601 gggagtgtca ttgactccgc ccgcctcgac gacagcaggc cagttgatga tcaaaggcac
    15661 gcgcacgccg ccttcatagg ggccgccctt gccgaggcgc agggggctgt tgtcggtggg
    15721 gccgcgccgc cagccctggg cgttgacgag gtggctgagg ccgccgttgt cgctggtgaa
    15781 aatgaagatc gtgttctcgt cgagggacag ctccttgagc gcggcgcgca ggcggcccac
    15841 gctgtcgtcc acgctttcga ccagcgcggc ataaactgga tcgtgatgat tgcccgccgg
    15901 gcctttggcg cggtatttgg cagcgacttc aggcttgccc atgatgggcg tgtggacggc
    15961 gtaatgcggc aggtagatga ggaacgggcg ctccttgttc cgctggatga actgaaccgc
    16021 ctcgctggtg aggcggtcgg tgaggaactc gccctcgggc ccgtcggtga gagtggggat
    16081 tttgtagggc gagaagtaac tcggcggcga tcccgcctca tagcctgcga tgttcacatc
    16141 gaagccatgc ttttccggcc caaattccgc gccgcccagg tgccacttgc cgatgctggc
    16201 ggtggcatag cccgcctccc tgagatgctc cgcgagggtg gtctcctcca aaggcagatg
    16261 cttcgtccat tcgggactct tgagccgcgt gtaagggcgc tgatgtccgg cgatccagtc
    16321 ggtgatgtga agccgggcgg gcgccttgcc ggtgagcacc gccgcgcgcg tgggcgagca
    16381 gacggtgcag gcggagtagg cgtccgtgaa gcgcatcccg tccctggcga gctggtcaat
    16441 gttcggtgtc tcataatact tgctcccctg gcagccgaga tcggtccagc cgaggtcgtc
    16501 caccagcatg agaatgacat tgggcgcgcg ctcctgggct gggagcagcg gggcgcagag
    16561 aagggcgaaa gcgagaagcg tgcggatcat ggcgggaaat gacgtgttgg tgtctcaatg
    16621 cacaccctgc tcagccaggt aacgctccgc ctccagcgcg gcggcgcagc cctgcccggc
    16681 ggcggtgatc gcctggcgat aaacgtggtc cgccacatcc cctgcggcaa agaggccctc
    16741 cgtcttcgtg cgcgtgcccc gtgtctgcag gatgtagccg ttttcatcgc gatccacgag
    16801 accgcccagg aacgcggcgt tcggcacatg gccgatggcg acaaagacgc acctcagctc
    16861 cagctcgctc ttttcaccgg tgacgagatc ctccagcgcc acggcgcgca tctcgccctt
    16921 gtcgtcggtc aggtattcgg tgacggcgct gttccagacc ggctcgatct tcgggttcgc
    16981 cagcgcgcgg tcggccatga tcttcgaggc gcgcagcgtg tccctgcggt ggatgaggta
    17041 aactttgctg gcgaacttgg tcaggaacat cgcctcctcg caggcgctgt cgccgccgcc
    17101 gatcacgcag accggcacat tgcggtaaaa ggccccgtcg caggtggcgc agctcgtcag
    17161 cccgtggccg atgagtccct tctcgttcgg caggccgaga tgcttgggcg aggcgccggt
    17221 ggcgatgatc accgcccgcg cctggcgcac cgtgccgtcc tccgtcttga tgtcgaagtg
    17281 cccgtccgcg gtcttcgcca cgctttccac cgtggcgtat tcgatgcgcg cgccgaactt
    17341 ctccgcctgc gtctgcatgt tcatcatcaa ctcggggccc atgatgccgt ttggaaagcc
    17401 ggggaaattc tccacctccg ttgtggtggt gagctgtccg ccaggcatgc tcccggatag
    17461 gatcagcggt ttcaggttgg cgcgtccggt gtagatggcc gcggtgtagc cggcgcagcc
    17521 ggtgccgatg atgatgacgt tttccatgag gtcgggtgtg ggaaggtggg cgaagatggg
    17581 gagtgcccgc gcgggcgcaa gacacacttt cggcgcggcc ttttgggagc gtcggcggcg
    17641 gcgggttatt ccgcgcgcgg ctgctcagcc gccgatcatg ccgcgcagct tctcctccgc
    17701 gtcctcgcac aggcggcgca gcatctccgg gctggtccag tccgcgccgc tggccagcac
    17761 cgtctcgcgc accatgccgt cggcggagac gacgcgcagc gggtggacgg gcgcgtattt
    17821 cgaggccagg cgctcgatga tggcatccgc cgtcgtgccc gcctccgcgt agatgacctg
    17881 gagtccgcct ggctcgcgct cctcggccct ggcctggtgc gtgccgtcga agacgagcac
    17941 cacctgcgcg ccgctcatgt cctgatagac gcgcagcacg cgcatcaatt ccagccgtgc
    18001 catgtttctg cgcgcggagt tgcgcatggc gctccgcagc tccggccagg cgtgcatcac
    18061 gctgtgtccg tccacgatca ggtaatgtga ggggccgcca tccatgagcg cgcaggtaag
    18121 caccgccgcg tccgatttcc cgcaaaatat gtctttccac cgccgccggg cgctggctat
    18181 aagcacagcc tcaaccacgc acccatgccc gccaagaaaa tcaacatcgc cgtcgtcggc
    18241 ctcggattcg gggctgagtt catccccatc tggcagcgcc acccgcacac cgcctgccac
    18301 gccgtctgcc agcgcgatcc gaagaagctc aaggctgtgg gagaggcctt cggtgtcggg
    18361 aagcgcttca cggatttcca cgagatgctc aatgatccgg agatagacgc cgtccacatc
    18421 aactcgccca tccccgacca cgcctggatg tccatcgccg cgctgaaggc gggcaagcat
    18481 gtggcctgca ccgtgcccat ggccaccagc atcgaggact gcaggaccat ctgcgaactc
    18541 gtgaggaaaa ccggcctcaa atacatgatg atggagaccg tggtctatgc gcgcgagttt
    18601 ctcttcatga gggagcaact ccacaaaggg aagctcggca aattgcaatt cctccaggcc
    18661 agccaccagc aggacatgga cggctggccg aactactggc ccggcctgcc gcccatgtgg
    18721 tatgccacgc actgcgtcgg ccccgtggcc gcgctggctg gcgcgccggc ggagtatgtg
    18781 agctgcttcg gctcgggcac catccgcccg gaactgatca agtgctacgg ctcgcccttc
    18841 gcagtcgaga ccgcgcatgt gaagttcaag ggcaccgacc tcagcgcgcg cgtcatccgc
    18901 agcctcttcg acaccgcgcg tcagtatcgc gagagcattg acgtctatgg cgacaaggca
    18961 tccatcgagt ggccgctcat cgagcacgag ccgctcgtca tgcacaccgc caagctgccc
    19021 gagcacaaga ttccgaagca tgtcaaagcg cccgactatg cgaaactgct gcccaagagc
    19081 atccgcgact tcaccaccaa gggcgtctat gacctggcca aaaagaccca cctcagcttc
    19141 acccagggca gcggccacgg cggcagccac ccgcatctgg cgcacgagtt cgccatggcc
    19201 ctcgtcgagg accgcgatcc cttcccgaac gcgaaacaaa gcgccaactg gacctgtgtc
    19261 ggcctctgcg cccacgagtc cgcgctcgcc ggaggcaaga tcgtcaagct ccccgccttc
    19321 acccagtgag ggcggccgcg tccgggcgga aaaccgcggc aacatcaagc aggcgtccgc
    19381 gttcccgtcg catgtcccga cttctgccct gcctcatcct cgcctccatc agtttcgcca
    19441 acggccagga gtcgaagaag gccagggcgg cggtgcagcc cttctcatgg gtgtccccca
    19501 tgcagaacgc cgagtgggcg aggaagtccc tgccagcgac ggtaaggcac accaccttca
    19561 aaagcgcctc gatgggcatc gaagtgggct gctacatcta tctgccgccg ggctatgaaa
    19621 gcagccagga gcgctacccg gtggtgtatc acctgcatgg cgggcggccg ggagcggaga
    19681 acaaagcggt gcggctggcc agccatgtgg atgccgccat tcaaaaaggt accatcaagc
    19741 cgacgatcta tgtcttcccc aatggcggcc cggtgagctg gtatgacatg ccggagatcc
    19801 cgaacggcct gggcgagacg gtgttcgtga aggagctggt gccgcacatt gacgccacct
    19861 gccgcacctg gggcacgcgc gaggggcggg cactggaggg ttactcgcag ggcgggcgtg
    19921 gcaccacgcg catcatgttc aagcacccgg agcttttcct ctccgtggcg cctggaggct
    19981 cgggctacgg gcctgaaaag cacatccagg aaaatgacgg ctacgagaat gacacgctgc
    20041 gcttcctgcc cgtgggctac aacgcctggg acctggccaa agcctacgcc gcgcgcgcgg
    20101 acaaacccgc gctgagcatc ctcgtctggg acggcaccaa gtgcttcaac tacgacttca
    20161 atctgcaata ctccgcctat ttgaaggagc tgggcatcgc gcatgagttc ctctccatcc
    20221 ccgacgtgcc gcacaccgcc accggcagct atgatgtgaa gggcgacgag atcatgcgcc
    20281 accaccagaa ggccttcgac gcccaccggc ccgcgcggtg aaataggacg gccttggcgg
    20341 cgttcccacc ggtccatgac cgacaaactc acccgacgcc acagcctcaa gctcctcacc
    20401 gccggctccc tcaccgcagg cctctccagc cgccactccc tgcccgcgca gacgccggac
    20461 gccaagcccg cgcgcgaccc ctgggcgaag acccacgacc gcgtcttcct cggcggcgaa
    20521 ttctgggcga accccatgga agactggcgc gtcagcgagg gcggcgcgga gtgcctgaat
    20581 gccgggcctg gacgcagcgt gcattccctc acccaccaga tcacccgcaa cggctccttc
    20641 accatggccg tcaccctcac ccggttggaa gcgtccgcca acgacggcgg cgccggcttc
    20701 cgcttcagcc tgcgcagcga gattgacgag caccgcagca actgcttcgt ccagcgcggc
    20761 cttgatgccg gctggcaggg ggaaaagctc atcctcgggc caaagaccgc tcccctccca
    20821 ggcggcgccg ccctgccgaa gctccgcctc accctcgccg gcgcacccca gggcggccag
    20881 tgcgccctca ctctcaccgc tgccgatgcc gccaccggcg cacccctcgc caccctcacc
    20941 cacaccgccc ccgcccaggc cgtcctcggc aatgtctccc tcgccaacag ctacgccccc
    21001 ggaggcgcgg gcgcggccaa gggcaagaaa ggcaaagccg gcgcggttac gggagggcgc
    21061 taccgcttcc aggattggac ctttgaagga gatgccttta ctgtcaccga gtcgcagcgc
    21121 ttcggcccca tcctctggtc catgtactcc ctcagcgact cccgcggccc cgaagctttc
    21181 gtcatgaaaa tgagcgccct caccggcccc ctcggcgcgc gcgacaatca cgaggtcgaa
    21241 ctccacatcc aaaaaggcgg cgcttggcaa tccctcggca cggcgaagct cgacaccgat
    21301 gcctggaccg ccaccttccg catcccgaac tgggacgaaa aacaggccac gccctacaag
    21361 ctcgtctatc aggaaaagca caccgacggc actaccacag cccatgagtg gaccggcacc
    21421 atcaaggcca accccgaagg ccgcccgctg cgcatggccg ccctcacctg ccagaacgac
    21481 tacggcttcc cctacgcccc tgtcgccgac aacgtccgca agctcgaccc cgacctcgtc
    21541 ttcttctccg gcgaccagct ctatgaaaac cacggcggct tcggcatcat ccgcgacgat
    21601 gccgggcgcg ccatcctcaa ctacctgcgc aagtt
//
LOCUS       DAOMYJ010000075        21495 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Prosthecobacter sp. isolate AMR_MDS_5629
            NODE_866_length_21495_cov_22.821875, whole genome shotgun sequence.
ACCESSION   DAOMYJ010000075 DAOMYJ010000000
VERSION     DAOMYJ010000075.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867850
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prosthecobacter sp. (wastewater metagenome)
  ORGANISM  Prosthecobacter sp.
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Verrucomicrobiaceae; Prosthecobacter.
REFERENCE   1  (bases 1 to 21495)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 21495)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.30
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:34:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,742
            CDSs (total)                      :: 4,684
            Genes (coding)                    :: 4,676
            CDSs (with protein)               :: 4,676
            Genes (RNA)                       :: 58
            tRNAs                             :: 55
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21495
                     /organism="Prosthecobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_866_length_21495_cov_22.821875"
                     /isolate="AMR_MDS_5629"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1965333"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1487)
                     /locus_tag="PLP58_09825"
     CDS             complement(<1..1487)
                     /locus_tag="PLP58_09825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ08857.1"
                     /translation="MNIRPLILAFCLPLLAGAADHAAWTHRQTVRVTQPGLTRIELDP
                     ALLDASRAASFHDLRLVSPDGVETPRIIALPRVVRPERVEAVGFKAMLAQAATVLEFQ
                     PRERDTIKEIVLRTSAVDFIKAAKVEASSDGGTWQTLGSGEVLCRQNGTERLRLPLAP
                     GLWTHFRVTLDDMRSAPVAFTGAEIVREIPELRSIPHDVAIRSRMEENGRTKLTLDLG
                     TANVLLATVRVLTPEPVFQREAELLSARATLFRLKHEDFTGEEIEIPVHRAAPAREIV
                     LTLHNGDSPPLRVEGIEAARHAVPLVFQAGAAGDWHLYIGNAQAAEPRYDIGALGDKL
                     RDASATTATAGKVEANPDFRAAATAPVVGELGSALDVSAWSYQREVLFAAPGVIELEL
                     DPAVLARSAGDLHDLRVVRDGRQIPFLVTRPDEKREITLTFSEIHDPKAPAWSKWEIE
                     MPLPNFPVSELLLDSSTPLFSRSLTLTEDAGGAQGRQQRVLGSAA"
     gene            complement(1484..4504)
                     /locus_tag="PLP58_09830"
     CDS             complement(1484..4504)
                     /locus_tag="PLP58_09830"
                     /inference="COORDINATES: protein motif:HMM:NF021606.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2339 domain-containing protein"
                     /protein_id="HRJ08858.1"
                     /translation="MEGLVILILLIVIVCIFVLPVIAIVKASGTREKAAQSERDIARL
                     REHNARLHERLERLEISVSALQTVPPAKAESRFAPPAAPVAQVTAKPVEPELVTAPLV
                     EKAPPPIPMPPPAPQTPEPVRAPVVAPVIAPPAAAKSAAPSKPVNLEQFMGVKLFAWV
                     GGLAFFLGVIFFVKLSIERGWISPELRTAIGFVIGAGLAGGGVVMHRRERYATLAQTL
                     CATGIVVLYGVTFAAHALWRIPPFDHAVAAFALMTLITAAAFLLAVRLNAQVVAVLGM
                     LGGFLTPILCSTGRDNPFGLFSYIALLDIGVLAVAKNKRWLHLTALAAAGTILMQAGW
                     MLKFFHSSQYAIGSATWVPVIVFLGFAALFSLAAWWSKQRDANDLFPAGSALALCGSA
                     MIAAFVFLGFGAITERPLLLYTFVLGINIIALRVVWHQSRVAIAQGVIGALTFLHLSI
                     WTMRELTSELLPHALGIYLAFGLLHTAFAVLMTRRDPDQPAFAGWVPLFTLVLLLMPV
                     LGLDAMSLMIWPAILIIDLLIIALAVVSGRLLPVLAALVLTLVTAAAWLFRWPGALAL
                     EMTPFLAVLGGFAVLFGIAGSLIFRKTPKAAFAPLLPVSSAVLPFLLLILATLRLKIA
                     DPSPVFGLALLLTVFLLGLVRISGITVLAPTALACVLALEWAWHQQSFHAEHATLPLL
                     WYLGFHAIFTAFPFVFQKRFATCKLPWITSAAAGIGTFSLVHRLVGQAWPNEMMGLLP
                     FAFAIAPLLSLVFVLKQHGAENPARLTQLAWFGGVALFFITLIFPIQFEKQWITLGWA
                     LEGAALIWLFRRVPHPGLRGTGAVLLAASFIRLALNPAVLSYQIRGGTPILNWQLYAY
                     SLSALAMFFAAFWLAPPSHLWGKINLRGLFCSLGGILLFLLLNIEIADAFTPVGERSI
                     TFDFSGNLARDMTYTISWALFALTLLVLGIWKRNAPTRYAGIGLLGVTLLKLFLHDLA
                     NTDSGYRIGALIGVAVIALAASFLYQRFLTADKS"
     gene            complement(4599..6200)
                     /locus_tag="PLP58_09835"
     CDS             complement(4599..6200)
                     /locus_tag="PLP58_09835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009960961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ08859.1"
                     /translation="MLQISQIKLPVDHTEARLRAAVLARLGVADGALLSLRVAQRAID
                     ARRGHVEFSLTVLVELADEEAVLIKAPPQVQPAPDDTYRELPVAVGAPPRPSGRPVIV
                     GTGPCGLFCGLLLARAGWRPVLLERGKAAGDRARDVTGFWRRGLAFDPESNVQFGEGG
                     AGTFSDGKLYTQIRDREHRIPWLLRELVAAGAPEDILIKARPHIGTDRLIKVVRHIRE
                     EIIALGGEVRFGARVSDIVVERGTMRAVTLADGAVVEGGPFVFAVGHSSRDTFAMLHR
                     HGVPLEPKPFSVGVRIEHPQRHIDRMLYGQRAGHPLLGSAPYKFVAHCPGGRSAYSFC
                     MCPGGLVVAATSEEGMVVTNGMSSYARAEANANSGFMVEVGPQDYGDSHPLAGVEYQR
                     EIERAAFRLGGGGYRAPAQLLDDFLSGRASSGAGDVRPSYQPGVVWTDLRECLPEKVV
                     LTLRQAVPLIEKKLRGFLLDDAVLTAAETRSSAPVRIPRHPGTTECTGLRNFHPAGEG
                     AGYAGGIISAAADGMRVAEALLRGN"
     gene            6331..7863
                     /locus_tag="PLP58_09840"
     CDS             6331..7863
                     /locus_tag="PLP58_09840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009961030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucan biosynthesis protein"
                     /protein_id="HRJ08860.1"
                     /translation="MIRHPSAGRMLLSAAFLAALDLHAADPGPEDVTDFPSLEAHAQA
                     LARAAHVPVEQDLDSFFAKLNYDGHRRIRFREDKALFGGAGDTFRVEFFHPGWMFKKP
                     VRFHQITDEKAEPVGFDSGLFDYGGLEVPANAGHPAGYAGFRVLAPDSFLDRRFEFLV
                     FMGASYYRAVTTELGYGISARGIAVNTVGGAPEEFPDFTRFWLARPKPGDDTFRLLAL
                     LDGPSLAGAYEFSAKPGRTTEMRVRGTLFLRQKVEMLGIAPFSSMFWYGENSHPKPYD
                     FRPEVHDSDGLQIEIENGPAIWRPLDVGRETRMSFFQAEKLKGFGLAGRDRDFANFQD
                     LEARYHQRPSVWVEPVAGFDKGAVVLVELPTGEETWDNIVAMWKPESVPQTPDEALRF
                     EYKLLWHGGHEPGGLCKVAATRRGFVMDSDDHLYVLDFEPGMAERGKAADWTPEARVR
                     VNAGAAEVLDARVMRNAESGGWRAFFKLDVPADTALLELICELHDGPLVISERWQYQW
                     RR"
     gene            7851..8429
                     /locus_tag="PLP58_09845"
     CDS             7851..8429
                     /locus_tag="PLP58_09845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ08861.1"
                     /translation="MEAVNIQFAPATGTEEEWNEAYARLADYFRSYQLHNRIRRTQLI
                     LETLRRAAEAHAKNPARTPTEHSIEQARLMLRDWLREIYRDLNLNDAQIEAAGRLGFY
                     LSGGPARWPHFFLDDENLPADMSEAMRAAVRTSGPGMQVSKMTPREIDLGIVSDVAED
                     TFDTLGRHPMLRYSILILMVGGVLGYLYLLLR"
     gene            8426..10594
                     /gene="mdoH"
                     /locus_tag="PLP58_09850"
     CDS             8426..10594
                     /gene="mdoH"
                     /locus_tag="PLP58_09850"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF003958.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucans biosynthesis glucosyltransferase MdoH"
                     /protein_id="HRJ08862.1"
                     /translation="MSVIGSLTTNLAATQGMPPPRIARTRRATFFSFVGLGTLAGVWL
                     MYVYLSEHGMRWSEWALLAAFVPLYYQLNTGFWTALIGVWLMNRPRPDPLDLWRSLTP
                     EEHEQPVTAGTAIVMPIYNEDVTRVFEGLRSIYLSLQETGHAANYDFFILSDSDQTSQ
                     WIEEESAWLELCRQLDAFGRIFYRKRRKPINRKSGNVSDFCRRWGRRYRYMVVLDADS
                     LMSGGLLNAMVRIMEKNPGVGILQTFPKQIGSETLLGRVMQFAQALYGPAFMAGLDYW
                     QCGEANFWGHNAIIRLDPFIKNCALPPLPGKEPFGGHILSHDFVEAALMRKAGYAVRL
                     LNTPRGSYEEGPPTLVDMLKRDRRWCQGNMQHFWLLFAKGWHPMSRINFLHGILSYAS
                     SLLWLVFLLLATFLAATPLPVESPQSALAARLLLGLTVMLIFLPKATILLDELITGRV
                     FKSPVMRALTFVSGLLDTVVFTLMAPVLMVFHSRFVIYTILGKGVRWVTQRRKLDSGV
                     DWVEVLLNFWPVSLLGVAWSVVAWFVSPGFLLWISPILVCLLAAIPVAVFVSGTRTGL
                     RFKLFLTPEEASPPQVLGTMRENLDEVKGRIQLQPELERFHGLMQVFLDPYINGLHVS
                     LLRRRRNIDVSRAYLNEIERRLIQEGPQSLTPQEIKAMIHDTDTVTNLHYRLWSAAEH
                     ELAPFWITAIRQYNLGSGGPFAHTLARKEPAVSEAFKAAS"
     gene            complement(10578..11051)
                     /locus_tag="PLP58_09855"
     CDS             complement(10578..11051)
                     /locus_tag="PLP58_09855"
                     /inference="COORDINATES: protein motif:HMM:NF019262.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ08863.1"
                     /translation="MNTRLLTLLSAALSAHLLVSCQHADEGSETRPAGENVLDRAALR
                     EAKSGTVSFESHVKPVLEAKCVACHQREALPGRLNLESRAAARRTGALGAFIVPGAPE
                     RSLFLTKLDEAHASVQAMPPVGETLTKDEVAILRRWIAQGADWPEGAAGMLKTRP"
     gene            complement(11117..12076)
                     /locus_tag="PLP58_09860"
     CDS             complement(11117..12076)
                     /locus_tag="PLP58_09860"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025217.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HRJ08864.1"
                     /translation="MPPPVAHLPALLSGPNRAANSLLGPFDELLEDFLSKGGPLPREY
                     GVLNRAIRHLGDAVRAGLIPAALLLEHIRSLTERRLAGTMQAQALAKKYGYSGDFEMI
                     DHIYVQKVNPDPGLRCWDLFFHAQAAPNAVRNRKRYFKNLVAEKLAGMPAGRRLDVLN
                     VASGPARDLREVFQDNEAADIHVDCVDMEGHAISYARQLCAPWLDRIEFHQRNVLRHM
                     PARGYDLAWSAGLFDYLGDRSFVHLLRAMLAVTRPGGEVVVGNFSDFNPSRDYMEMMG
                     DWRLRHRSQETLTSLACQAGIPPNRLRIFWEPEGVNYFLHARA"
     gene            12285..12914
                     /locus_tag="PLP58_09865"
     CDS             12285..12914
                     /locus_tag="PLP58_09865"
                     /inference="COORDINATES: protein motif:HMM:NF014269.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LON peptidase substrate-binding
                     domain-containing protein"
                     /protein_id="HRJ08865.1"
                     /translation="MNPIPAAPAYPLPDTLPVMVLADCHLMPCCLLPLFIFEERYRKM
                     LAHALACHRMFCIGTRLGADGDDLEIFPFSTAGLISVCKTQPDGTSHVILQGLRRIRL
                     VSWEQEKPFIVARVEPVTPSVSECVEKIQSLKQQALDLLPDIPGNAGENIRQLKARLG
                     GTTCVDSACDILAYHFVRRGECMRALLAETVVEKRWRILIRELQQMRAA"
     gene            12927..13832
                     /locus_tag="PLP58_09870"
     CDS             12927..13832
                     /locus_tag="PLP58_09870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018969037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0009037 - tyrosine-based site-specific
                     recombinase activity [Evidence IEA];
                     GO_process: GO:0006313 - transposition, DNA-mediated
                     [Evidence IEA];
                     GO_process: GO:0007049 - cell cycle [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HRJ08866.1"
                     /translation="MPHPAPPDDPLKKAFLGFLESERRCSPRTLENYRHALAEFRHRQ
                     SGFTAWLTLTAEDFRGYLFDQMKRGLGRATIRLHFAALRSFYKWLTHRQGLERNPLLD
                     VQLPKQEKKLPVVLTVPQITAMLELPLKLEKDRQAPAWAPERDAAVLELFYSTGMRLA
                     ELSALNVADLDAYSDTVRVTGKGSKQRLLPVGAPAMEAVQRYRQKAAVHDGPLFLSKL
                     RRRITTQAVADIVDKYWRKSGLPVHVTPHKLRHSFATHLLDNGADLRSVQELLGHASL
                     STTQIYTHVSTQRMKEVYEAAHPRA"
     gene            13866..14786
                     /locus_tag="PLP58_09875"
     CDS             13866..14786
                     /locus_tag="PLP58_09875"
                     /inference="COORDINATES: protein motif:HMM:NF027552.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4886 domain-containing protein"
                     /protein_id="HRJ08867.1"
                     /translation="MIPRLFLLLLLLPVGGAAETVRLLTIGNSFSRNATNHLGDLAKA
                     GGHELIHESIVVGGASLELHSEKALLHEKDPAHKDGLYTSGRSLAQHLRQERWDFVSI
                     QQASIKSHDFATYEPHGSRLAALIRSHAPGARILVHQTWAYRVDDPRFTKPSDTDGEP
                     ATQEQMYRGLTAAYDQLASLIGARVIPVGDAFFAADTDPQWGYKPGSVPFDPKTARRP
                     ALPEQTHSLHVGWRWAKARNSVKITLGMDGHHANLAGEYLGACVWYEVLFGDTPPGST
                     HLPKGLDAAHARFLQETAHHAVQARLASGQ"
     gene            15006..19787
                     /locus_tag="PLP58_09880"
     CDS             15006..19787
                     /locus_tag="PLP58_09880"
                     /inference="COORDINATES: protein motif:HMM:NF024144.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cadherin-like beta sandwich domain-containing
                     protein"
                     /protein_id="HRJ08868.1"
                     /translation="MNTSAPANILRRSLPCVLIGILMLCLPRQVEAQVPPYLLRHSIP
                     APLEAQYGGNLGFSCAQSGNLAALGAPYDDTGAQDAGVVKIFDTTTGDLVQVVPNPTL
                     GTYGYFGTAIAMEGARLVVGAPNQFAGASGAGAVYVYDLGGPTPTTPILTIPNPAPAS
                     YDSFGSALALSGDRLVVAAEWDDAGAADAGSAYVFDLGSGTPATPVFTLSNPNPAASD
                     RFGISVAVDGDRVVVGVLFADLGASNAGRAYVYDLGGMTPGTPVLSLSNPAPTADAYF
                     GRSVGISGQIVAVGAHNDRTGPQGTGSVHVYNLGSGTPETPVHAIFNPGPGSNEAFGS
                     TLVLRGKRLLVGDFVENTGATASGTAYLYELASGSPTVPAFTFNNPNPGAFDRFSWGI
                     SLADDQVLIGAYTDDKGGIDAGTAYFYDLLSGTPTLPVREFNDPGFEVLSQFGQSVAV
                     SGTLVAVGTGEKDLNIDGAGQVRIFDTSSPTPNVPVLTIPSPQPALRGRFGVAVALDG
                     TRLVVGARDMNGSVVFAGAVYVYDLGSATPGTPVQVIPNPVPSSSSNRFGRSVALSGT
                     HVVVGAPEEDTLAANAGSAYVFDLSSGTPGVPVLTLDHPTAGDLFGFSVGISGTRIIV
                     GAHENDTGASNAGSAYVYDLASGTPATPVAALNNPTPASNDFFGCTVAIEGGRAVVGA
                     YGDDTGAQNAGSVYVYDLDGATPTVPLNVINSPAVISGEEFGRALALSGGRLVVGAPG
                     NDTGANNTGIAYVYNLAGASPTVPEDTLENPAPGISDNYGFSVAIHQQRIVVGAPLSD
                     LSGMNQGAVHVFSPPSMDASLANLEVSQGTLMPGFAPGTTAYTLSLPPGTSSMTLTPT
                     AADAGAVITVNGSAVVSGQASGSLPLIFGDNLISIVVTAEDGTTQRTHTLTVTAPGFS
                     TLAFASKVIPVASNAAGSMAEVMIQRTGNLDGMASCTLTSADGSATAPAHYTAQSSLQ
                     VDFGDGQDTKQVQIPIAADAAATVARAFTITLADASTGVALGQPSAATVVILPPAAFT
                     EKVKPVVKFTAPANNAVVVDTVPVTVSGMATDNIGIHKVQVSLNNGPFVDANVASPGG
                     TSTEWNLNVTPAPGKNTLKVRSVDFKGNLSSVISRGFTHLRTLTVAINGPAGSGTVST
                     GFMPSSKRIAGKSYSITATPKPGFVFDGWTASDTAGTGITVASAELPKLAFIMQPGLS
                     LTAKFIVNPFDAQLTGTYRGLVRPDMQTDENNSTAGLVHNVVVTSKGAFSGSLLMDGA
                     KIKLAGFFDNTGDARFGPARSKTLVVTRKNKPVMQISLHLDMTGFTHRITGGVDQLDQ
                     SVLVAHSDITADRAAFGKLTEAPAALASKNGKSYTLVFAAKTQTPAIPLNLYPQGHGH
                     ASVIIKSTGAVTLSGKLADGTIFTSATTLSKHLQFPAFAQLYQMQGSFGGEITVADAG
                     QNADDLTGVMFWFRPQVNGSQWYQAGWPDGIGTDALGNRYTVPSGSSVFPGLQNLPAN
                     ADLSLSGGLLAPSILQDMLITPANKVSPVPAAALPTAVITAKTGGIAGKFRHSLGAST
                     AYQGVILQKGTRTGAHGWFLSPKPAKPDFLGESGAMRMTAK"
     gene            19894..>21495
                     /locus_tag="PLP58_09885"
     CDS             19894..>21495
                     /locus_tag="PLP58_09885"
                     /inference="COORDINATES: protein motif:HMM:NF019219.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1549 domain-containing protein"
                     /protein_id="HRJ08869.1"
                     /translation="MPRLFFIITVLLWTGRASLPGAVEDPSEFFEKRVRPLLLEHCTE
                     CHGADKQKGSLRLDHKAGWERGGDSGPALAPGNLDGSLLWRAVGYEDRDLKMPPKKRL
                     PESALADLKAWITSGAHDPRDAPPEAGGGKSGPKADGSFWSFQLPKLHAPPTVQKNDW
                     PARPLDHFILARLEQNQMTPAPDADARTLARRLHFDLTGLPPSHEEVESLAQGKVGYE
                     ERVDALLASPAFAERWTSHFMDMTRFAESSGGGRSLPFKDAWRFRDYLLESLRDGVPV
                     DRMIQEMTAGDLLPFENAAQRRRQITATGFLALGPTNYEEQDKQLLRMDIVDEQLDTI
                     GKSFLGMTIGCARCHDHKFDPVSTRDYYALAGIMRSTQTLKNYTDNVAHWIDTPLPYE
                     GELEEQMRAKEEKLAELNKQLASLKDELRDIGGANLRKKNSIAVTDLPGIVIDDSAAQ
                     RVGNWKESMLVKPFIGEGYVHDKTGDTGERTISFSPKIPVTGRYEVRVAYIGGPDRAE
                     KVRADVLHADGEETASYSTRTASMEG"
BASE COUNT         4100 a         6945 c         6802 g         3648 t
ORIGIN      
        1 catgcggcgc tgccgagcac ccgctgctgg cggccctgcg cgccgccggc atcctcggtc
       61 aaggtgagcg agcgggaaaa aagaggcgtg gaagagtcga gcagaagttc ggagaccggg
      121 aagttgggca gcggcatctc gatctcccat ttcgaccacg ccggcgcttt gggatcgtga
      181 atctcggaga aggtcagtgt gatctcgcgt ttctcatcgg gccgggtgac gagaaaggga
      241 atctggcgtc cgtcacggac cacgcgcagg tcgtggagat caccggcgct gcgcgcgagc
      301 acggcgggat cgagttccag ctcgatgacg cccggggccg cgaacaaaac ctcgcgctga
      361 tacgaccacg ccgagacatc aagcgccgac cctagttcgc cgaccacggg cgcggtggcg
      421 gcggcgcgga aatcggggtt ggcctcgact tttccggcgg tggctgtggt ggcgcttgca
      481 tcgcgcagct tgtcgccgag cgcgccgatg tcatagcgcg gctcggcagc ctgggcgttg
      541 ccgatgtaga ggtgccagtc tccggcggcg ccggcctgga acaccagcgg cacggcatgg
      601 cgcgcggcct cgatgccttc aacgcgcagc ggcgggctgt cgccattgtg aagggtgagc
      661 acgatctcgc gtgcgggagc ggctcgatgc accgggattt cgatttcctc gccggtgaag
      721 tcctcgtgct tgaggcggaa caaggtggcg cgcgcgctca gcagctcggc ctcgcgctga
      781 aagaccggtt cgggagtgag gacacgcacg gtggcgagca ggacattggc ggtgccgaga
      841 tcgagcgtca gcttcgtgcg tccattctct tccatgcgcg agcgtatcgc cacatcatgc
      901 gggatggagc gcagttccgg aatctcgcgc acgatctccg caccggtgaa ggccacgggc
      961 gcgctgcgca tgtcatcaag ggtgacgcgg aaatgcgtcc acaagccggg tgcgagcggc
     1021 aggcgcagac gctcggtgcc gttttgacgg cacaaaacct ccccgctgcc gagggtctgc
     1081 caggtgccgc cgtcactgct cgcctccact ttggcggcct tgatgaaatc cactgcgctg
     1141 gtgcgcagca cgatctcttt gatggtgtcg cgctcgcgag gctggaactc aagcacggtg
     1201 gccgcctgag cgagcatggc tttgaacccg accgcctcca cgcgctcggg gcgcaccacg
     1261 cgcgggaggg cgatgatgcg cggggtctcg acgccgtcag gactgacgag gcgcaggtcg
     1321 tgaaacgacg ccgcgcgcga ggcgtcgagc agggcgggat caagctcgat gcgcgtgagt
     1381 ccgggctgcg tgacgcgcac cgtctggcgg tgggtccatg cggcgtggtc ggcggctccg
     1441 gcgagcaagg gcaggcaaaa ggcgaggatg agcgggcgaa tgttcatgat ttgtcagcgg
     1501 tcaggaagcg ttgatagagg aaggaggccg cgagcgcgat gacggccacg ccgatgaggg
     1561 cgccgatgcg gtagccgctg tccgtgttcg ccagatcgtg caggaagagt ttcagcaggg
     1621 tgacgccgag caggccgatg ccggcgtagc gcgtgggcgc gttgcgtttc cagatgccga
     1681 gcacgagcaa ggtgagcgcg aacaaggccc acgagatcgt gtaagtcatg tcgcgggcca
     1741 gattgccgct gaaatcgaag gtgatcgagc gctcgccaac gggtgtgaag gcgtcggcga
     1801 tctcgatgtt gagcagcagg aacagcagga tgccaccgag cgagcaaaac aggccgcgca
     1861 ggttgatttt accccagaga tgacttggag gcgcgagcca gaaggcggcg aagaacatcg
     1921 ccagcgcgga gaggctgtaa gcataaagct gccagttcag gatgggggtg cctccacgaa
     1981 tttgatacga gagcacggcg ggattgagcg cgagacggat gaacgaggcc gcgagcagca
     2041 cggcgccggt gccgcgcagg ccgggatgcg gcacgcgccg gaaaagccag atcaacgccg
     2101 cgccctccag cgcccagcca agcgtgatcc actgcttctc gaactggatc gggaagatga
     2161 gcgtgatgaa aaacaacgcc acacctccaa accaggcgag ctgcgtgagc cgagccggat
     2221 tttcagcgcc gtgctgtttc aagacgaaga caaggctcaa caaaggcgcg atggcgaagg
     2281 cgaagggaag cagtcccatc atctcattcg gccacgcctg gcccacgagg cgatgcacga
     2341 ggctgaacgt gccgatgcct gccgccgcgg aggtgatcca cggcagcttg catgtggcga
     2401 accgcttctg aaacacgaac gggaacgcgg tgaagatggc gtggaagccg agataccaga
     2461 gcagcggcag cgtcgcgtgt tccgcatgga aactctgctg atgccaggcc cattcgagcg
     2521 cgagaacgca ggcgagtgcc gtcggtgcca gcacggtgat gccgctgatc cgcaccaggc
     2581 cgagcaggaa gaccgtgagc aacaatgcga ggccgaaaac cggcgaaggg tccgcgatct
     2641 tcaaccgcag cgtggccaga atgagcagca ggaaaggcag taccgccgaa ctcaccggca
     2701 gcagcggcgc gaaagcggcc ttcggcgttt tgcggaaaat caaactgccc gcgatgccga
     2761 acagcaccgc gaagccgccg agcaccgcaa ggaagggagt catctcaaga gccagcgcgc
     2821 ccggccagcg gaagagccag gcggcggcgg tgaccagcgt gagcacgagc gcggcgagta
     2881 ccggcagcag gcgtccgctg accacggcga gggcgatgat gagcagatca atgatgagga
     2941 tcgccggcca gatcatcagg ctcatggcat ctaggcccag caccggcatg agcaggagca
     3001 ctagcgtgaa caggggcacc cacccggcga aggccggttg atccggatct ctgcgcgtca
     3061 tgagcacggc gaacgcggtg tgcagcaagc cgaacgccag ataaatgccc agcgcgtgcg
     3121 gaagcaactc cgaggtcagt tcacgcatcg tccagatcga gaggtgcagg aaagtcagcg
     3181 cgccgatgac gccctgcgcg atggcgacgc gcgactgatg ccacacgacg cgcagcgcga
     3241 tgatattgat gccgagcacg aaggtgtaga gcaacagcgg acgttccgtg atggccccga
     3301 agccgagaaa aacgaacgcg gcgatcatgg cgctgccgca caacgcgagc gcggagcctg
     3361 ccgggaaaag atcgttggca tccctttgct ttgaccacca agcggcgagt gagaacagtg
     3421 ctgcgaaacc gaggaacacg atcaccggca cccaggtggc cgaaccgatg gcatactgcg
     3481 aggagtggaa gaatttcagc atccacccgg cctgcatgag gatggtgcca gccgctgcaa
     3541 gcgcggtgag atgcagccag cgtttgtttt tcgccacagc gagcacgccg atgtcgagca
     3601 gagcgatgta gctgaaaagg ccgaagggat tgtcccggcc cgtggagcaa agaatgggcg
     3661 tcaaaaagcc tcccaacatg cccagcacgg ccacgacctg cgcgttcaag cgcacggcga
     3721 gaaggaatgc ggcggcggtg atgagcgtca tcaacgcgaa cgcggcgacg gcatggtcga
     3781 atggcgggat acgccacagc gcgtgcgctg cgaaggtgac gccgtagagc acgacgatgc
     3841 ccgtggcgca cagcgtctgg gccagcgtgg catagcgctc cctgcggtgc atgaccacgc
     3901 cgccgccggc aagacccgcg ccgatgacaa aaccgatggc cgtgcgcagc tcgggtgaaa
     3961 tccagccgcg ttcgatgctg agcttcacaa agaagatgac gccgaggaag aacgccagac
     4021 ctcccaccca tgcgaacagc ttcacgccca tgaactgctc cagattgaca ggtttcgacg
     4081 gagcggcgga tttggcagca gcgggcgggg cgatcactgg agcaaccaca ggcgcgcgga
     4141 caggctcggg cgtttgcggc gcgggcggcg gcatcgggat cggcggcggc gctttttcga
     4201 caagcggcgc ggtgacaagt tccggctcca cgggctttgc tgtgacttgc gcgaccggcg
     4261 cggctggagg agcgaatcgg gactccgctt tggcaggcgg cacggtttgc aaggcgctga
     4321 cggaaatttc aagacgctcc agacgctcat gaagccgcgc gttatgctcg cgcagcctgg
     4381 cgatgtcgcg ctcgctctga gcggcctttt cacgagtgcc ggatgctttg acgatggcaa
     4441 tgaccggcag cacaaagatg cagacgatca caatcaggag gatgagaatg acgagtcctt
     4501 ccataatgcg ccgagtgtcc caggtttgtc cgggaatgga aattgatatt tccaggcggc
     4561 atcattgatc atccgtatgg acgggccatc cgcaggtctc agtttccgcg cagcagcgcc
     4621 tcggccactc tcatgccgtc agcggcggcg gagatgatgc cgccggcata gcccgcgccc
     4681 tcgccggcag ggtggaagtt gcgcaggccg gtgcattcag tggtgccggg gtggcgcggg
     4741 atgcgcacgg gggctgagct gcgtgtctcg gcggcggtga gcacggcgtc gtcaagcagg
     4801 aagccgcgca gttttttctc aatgagcggc acggcctggc ggagggtgag gacgaccttt
     4861 tcaggcaggc actcgcgcag atccgtccac acgacaccgg gctggtagga ggggcgcaca
     4921 tcccccgccc cgctggaggc gcggccggaa aggaagtcgt ccagcaactg cgccggggcg
     4981 cggtagccgc cgccgccgag acggaaggcg gcgcgctcga tctcgcgctg gtattcgacg
     5041 ccggcgaggg gatgcgagtc gccatagtcc tgcgggccga cctcgaccat gaagccgctg
     5101 ttggcattgg cctcggcgcg ggcgtagctg ctcatgccgt tggtgacgac catgccctcc
     5161 tcggaggtgg cggcgacgac gaggccgccg gggcacatgc agaagctgta ggcgctgcgc
     5221 ccgccggggc agtgcgccac gaatttgtaa ggagcgctgc ccagcagcgg gtggccggcc
     5281 cgttggccgt aaagcatgcg gtctatgtgc cgctgcggat gctcgatgcg cacgccgacg
     5341 gagaagggct ttggctccag gggcacgcca tgccggtgca gcatggcaaa ggtgtccctg
     5401 ctgctgtgtc cgacggcgaa tacgaaaggc ccgccctcca cgaccgctcc atccgccagc
     5461 gtcacggcgc gcatggtgcc gcgctcgacg acgatgtcgc tgaccctcgc gccgaagcgc
     5521 acttcgccgc cgagggcgat gatctcctcg cggatgtggc gcacgacttt gatgaggcgg
     5581 tcggtgccga tgtgagggcg ggctttgatc aaaatgtcct ccggagcgcc cgccgcgacc
     5641 agttcgcgca gcagccaggg aatgcggtgc tcacggtcgc gtatctgggt gtagagcttg
     5701 ccatcggaaa aggtgcccgc ccctccctcg ccgaactgca cgttggactc ggggtcgaag
     5761 gccaggccgc gccgccagaa gccggtgaca tcgcgcgcgc ggtcgcccgc cgccttgccg
     5821 cgctccagca aaacgggacg ccagccggcg cgagccaaaa gcagcccgca gaaaagcccg
     5881 cacgggccgg tgccaacgat gacgggcctg ccggacgggc gcggcggcgc tccgactgcg
     5941 acgggcagtt cacggtaagt atcgtccggg gctggctgaa cctgcggcgg ggcttttatc
     6001 aacaccgcct cctcatcggc cagctcgaca agaacggtga ggctgaactc cacatgcccg
     6061 cgccgcgcgt caatggcgcg ctgggccacg cgcagggaaa gcagcgcgcc atcggcaacg
     6121 cccagccgcg cgagcacggc tgcgcgcagc cgggcctccg tgtggtccac ggggagcttg
     6181 atctgggaga tttgcagcat ggcggcgctt gtttgcgcag gcagccgccc catgcaagcg
     6241 cgcttcatct gaagcactgg cacactttat gccactgctt gacggacggg cgctttatgg
     6301 cactctccgc cgccgctgtc cgcagcctat atgattcgac acccctccgc aggacgaatg
     6361 ctcctgtccg ccgccttcct ggccgccctc gacctccatg ccgccgatcc agggccggaa
     6421 gatgtcacgg attttcccag cctggaagcc catgcccagg cgctggcgcg cgccgcgcat
     6481 gtcccggtgg agcaggacct ggactcattt ttcgcaaagc tgaactacga cggccaccgg
     6541 cgcattcgct tccgcgagga caaggcgctc ttcggcggcg cgggtgacac gttccgtgtg
     6601 gagttttttc atccgggctg gatgttcaag aagccggtgc gctttcacca gatcacggac
     6661 gaaaaggcgg agcctgtggg ttttgacagc ggcctctttg actatggcgg gctggaggtg
     6721 ccggcgaacg cgggacatcc tgccggttat gccggcttcc gcgtgctggc tccggattcg
     6781 ttcctggacc ggcggttcga gttcctggtg ttcatgggag cgagctacta ccgggcggtg
     6841 acgacggagc tgggctacgg catctcggcg cgcggcatcg cggtgaacac cgttggcggc
     6901 gcgccggagg aattcccgga tttcacacgc ttctggctgg cgcggccaaa gccgggggat
     6961 gacactttcc gcctgctggc gctgctggac gggccgagcc tggcgggcgc gtatgagttt
     7021 tcggcgaagc cgggcaggac gacggagatg cgagtgcgcg ggacgctttt cctgcggcaa
     7081 aaggtggaga tgctgggcat cgctcctttt tccagcatgt tctggtatgg cgagaacagc
     7141 cacccgaagc cgtatgactt ccgtccggag gtgcatgact cggacggcct gcaaatcgaa
     7201 atcgagaacg gcccggcgat ctggcgtccg ctggatgtgg ggcgggagac gcggatgagt
     7261 tttttccagg cggaaaagct caagggcttc ggtctggcgg ggcgtgaccg ggactttgcg
     7321 aacttccagg acctggaggc gcgctatcat caaaggccgt cggtgtgggt ggagccggtg
     7381 gcgggttttg acaaaggcgc ggtggtgctg gtggaactgc cgaccgggga ggagacatgg
     7441 gacaacatcg tggccatgtg gaagccggag agcgtgccgc agacgccgga tgaggcgctg
     7501 cgatttgaat acaagctgct gtggcacggc ggccacgagc ctggcgggct gtgcaaggtg
     7561 gcggccaccc gccggggctt tgtcatggat tcggacgatc atctgtatgt gctggatttc
     7621 gaacccggca tggcggagag aggcaaggcg gcggactgga ccccggaggc gcgggtgcgg
     7681 gtcaatgccg gcgccgcgga ggtgctggac gcgcgcgtga tgaggaacgc ggagtccggc
     7741 ggctggcggg cgtttttcaa actcgacgtg ccggctgaca ccgccctgct ggagctgatc
     7801 tgcgagctgc atgacgggcc tttggtgatc tccgaacgct ggcaatacca atggaggcgg
     7861 taaacatcca gttcgcgccc gccacgggca ccgaggagga gtggaacgag gcgtacgcgc
     7921 gtctggccga ctacttccgc tcctaccagc tccacaaccg catccggcgc acgcagctca
     7981 tcctggagac tctgcgccgc gcggcggagg cccatgccaa aaaccctgcg cgcacgccga
     8041 cggagcactc catcgagcag gcgcgcctga tgctgcggga ctggttgcgc gagatttacc
     8101 gcgacctgaa tctcaacgac gcgcaaatcg aggcggcggg caggctgggc ttttacctca
     8161 gcggcggtcc ggcacgctgg ccgcattttt ttctggatga tgaaaacctg cccgcggaca
     8221 tgagcgaggc gatgcgcgcc gctgtgcgca cctccggccc tggaatgcag gtgtccaaaa
     8281 tgacgccgcg cgagattgac ctcggcattg tctccgacgt ggccgaggac accttcgaca
     8341 cgctcggcag gcaccccatg ctgcgctatt ccatcctcat cctcatggtc ggcggcgtgc
     8401 tcggctatct ctacctgctt ctgcgatgag cgtcatcggc tccctcacca ccaatctcgc
     8461 ggccacgcag ggcatgcccc cgccgcgcat cgcccgcacg cggcgcgcca cgtttttctc
     8521 ctttgtcggg ctgggcaccc tcgcgggcgt ttggctcatg tatgtctatc tctccgagca
     8581 cggcatgagg tggtcggagt gggctctgct ggcggccttt gtgccccttt actaccagct
     8641 caacacgggc ttctggacgg cgctcatcgg agtctggctg atgaaccgcc cgcgccccga
     8701 tccgctcgac ctctggcgca gcctcacacc cgaggagcac gagcagccgg tgacggcggg
     8761 cacggccatc gtgatgccga tctacaacga ggacgtcacc cgcgtgttcg agggtctgcg
     8821 ctccatttac ctctccctcc aggaaaccgg ccacgccgcg aactacgatt ttttcatcct
     8881 cagcgactcg gaccagacga gccagtggat cgaggaggaa agcgcctggc tggagctttg
     8941 ccggcagctc gacgccttcg gacgcatctt ttaccgcaag cgccgcaagc cgatcaaccg
     9001 caagagcggc aatgtgagcg acttctgccg ccgctggggc cgccgctacc gctacatggt
     9061 cgtgctggac gcggacagcc tgatgtccgg cggactgctg aacgcgatgg tgcgcatcat
     9121 ggagaagaac cccggcgtgg gcatcctgca gacttttccg aagcagatcg gctccgagac
     9181 gctgcttggg cgcgtgatgc agttcgccca ggcgctgtac ggccccgcct tcatggccgg
     9241 cctggactac tggcagtgcg gcgaggcgaa cttctggggg cacaatgcca tcatccgcct
     9301 cgatcctttc atcaaaaact gcgccctgcc gccgcttccg ggcaaggaac ccttcggcgg
     9361 ccacatcctc agtcatgact tcgtggaggc cgccctcatg cgcaaggccg gctacgccgt
     9421 gcggctgctg aacacgccgc gcggcagcta cgaggagggg ccgccgacgc tggtggacat
     9481 gctcaagcgc gaccgccgct ggtgccaggg gaacatgcag catttctggc tgcttttcgc
     9541 caaaggctgg catccgatga gccggatcaa tttcctgcat ggcattttaa gttatgccag
     9601 ctcgcttcta tggctggtct tcctgctgct ggccaccttc ctggccgcga cacccctgcc
     9661 ggtggaaagt ccgcaatccg cgctggcggc gcggctattg ctggggctga cggtcatgct
     9721 catctttctg ccgaaggcca cgatcctgct cgacgagctg atcaccgggc gcgtcttcaa
     9781 atcaccggtg atgcgcgcgc tgacctttgt cagcggcttg ctggacacgg tggtcttcac
     9841 gctgatggcg ccggtgctca tggtcttcca cagccgcttt gtcatctaca cgatcctcgg
     9901 caagggcgtg cgctgggtga cgcagcggcg gaagctggac agcggggtgg actgggtgga
     9961 ggtgctgctg aatttctggc cggtcagtct gctgggggtg gcgtggtcgg tggtcgcctg
    10021 gtttgtctca ccgggctttt tgctctggat cagccccatc ctcgtgtgcc tgctggccgc
    10081 gattcccgtg gcggtgtttg tctccggcac gcgcaccgga ctgcgcttca agctcttcct
    10141 cactccggag gaggcatcac cgccgcaagt gctcgggacg atgcgggaga acctcgacga
    10201 ggtgaaaggc cgcatccaac tgcagccgga actggagaga ttccacggcc tcatgcaggt
    10261 cttcctcgac ccctacatca acggcctgca cgtctccctg ctgcgccggc ggcggaacat
    10321 tgatgtttcc cgcgcctacc tcaacgagat cgagcggcgc ctgatccagg aagggccgca
    10381 aagcctgaca ccgcaggaga tcaaagcgat gattcacgac accgacacgg tgacaaacct
    10441 gcattaccgg ttgtggtccg ccgccgagca cgagctggcg ccgttttgga tcacggccat
    10501 ccggcagtac aacctgggat cgggcggccc cttcgcccac acgctcgcgc gcaaggagcc
    10561 ggcggtctcg gaagctttca aggccgcgtc ttgagcatgc ccgccgcgcc ctccggccag
    10621 tccgcgccct gggcgatcca gcggcgcagg atggcgacct cgtccttggt gagcgtctca
    10681 cccactggcg gcatcgcctg cacgctggcg tgagcttcgt cgagcttggt gaggaacagg
    10741 ctgcgctccg gcgcgcctgg cacgatgaag gcgccgagcg cgccggttcg gcgggctgcg
    10801 gcgcggcttt ccaggttcag cctgccgggc agtgcctcgc gctggtggca ggccacgcat
    10861 ttggcctcaa gcacgggttt gacatgactt tcaaaactca ccgtgccgct tttcgcctcg
    10921 cgcagcgcgg cgcggtccag cacgttttct cccgcgggcc gcgtctcgct gccctcatcc
    10981 gcgtgctggc atgagacgag cagatgggcc gacagcgcgg cgctgagcaa agtgagaagg
    11041 cgcgtgttca caatggaatg aggtgtgttt tttgaaacta gcctgccgaa aaggccgcgc
    11101 catgaagaaa tgcgcctcac gcccgcgcgt ggagaaagta attcactccc tccggctccc
    11161 aaaagatgcg cagccggttg ggaggaatgc ccgcctggca cgcgaggctc gtcagggtct
    11221 cctggctgcg gtggcgcagg cgccagtcac ccatcatctc catgtagtcg cggctgggat
    11281 tgaaatcgga gaaattcccc accaccacct cgccaccggg ccgggtgacg gcaagcatgg
    11341 cgcgcagcag atgcacaaag gagcggtcgc ccaggtaatc gaacagcccc gccgaccatg
    11401 caaggtcgta accgcgcgcg ggcatgtggc gcagcacatt gcgctggtga aattcaatcc
    11461 ggtccagcca cggcgcgcac aactgccgcg cataactgat ggcgtggccc tccatgtcca
    11521 cacagtcaac atggatgtcc gccgcctcgt tgtcctggaa gacctcgcgc aaatcccgcg
    11581 cgggaccgct ggcgacattg agcacatcca ggcggcgccc ggcgggcatc ccggccagct
    11641 tctcggccac caagttttta aagtagcgtt tgcggttgcg aaccgcgttc ggagccgcct
    11701 gcgcatggaa aaacaaatcc cagcagcgca gtccgggatc gggattcacc ttctgcacat
    11761 aaatatggtc aatcatctcg aagtcgccgc tgtagccgta tttcttcgcc agcgcctgcg
    11821 cctgcatcgt gcctgcaaga cgccgctccg tcagagagcg gatgtgctcc agcaacaggg
    11881 ccgccgggat cagacccgcg cgcacagcat cgccaaggtg ccggatggcg cgattgagca
    11941 cgccatactc cctgggcagc ggtccgccct tcgaaagaaa gtcctccagc aactcatcaa
    12001 agggaccgag cagggagttc gccgcacgat tcggacctga cagaagagca ggaagatggg
    12061 cgacgggtgg cggcatggca gattgttcta aaatatcctg gataaatgat atttaacaga
    12121 cactttttat taaaatatcc tgatcaatgc ggcttggcaa tttcgaaaac atccggagcg
    12181 ggatcaatcc accgcgcaac aaccccaaac ctgccctctt cccatccccg caaactcatg
    12241 tccaaacccg cgctccagcc gtaacaccca ggctatccga ttccatgaat cccatccccg
    12301 ccgccccagc ctatcccctg ccggacaccc tcccggtcat ggtgctggcg gattgccatc
    12361 tcatgccctg ctgcctgctg ccgctgttca tcttcgagga gcgctatcgc aaaatgctgg
    12421 cgcatgccct ggcctgtcac cgcatgtttt gcatcggcac ccggcttggc gctgacgggg
    12481 acgacctgga gatattcccc ttcagcaccg ccggcttgat cagcgtgtgc aaaacccagc
    12541 cggacggcac atcgcatgtc attcttcagg gcctgcgccg catccggctg gtctcgtggg
    12601 aacaggagaa gcccttcatc gtcgcgcgtg tcgagccggt cacgccctct gtcagcgaat
    12661 gcgtggagaa gatccagtcg ctcaagcagc aggctcttga tctgctgcca gacattcccg
    12721 gcaatgccgg ggaaaacatc cgccagctca aggccaggct gggcggaacc acctgcgtgg
    12781 actccgcctg cgacatcctg gcctatcact ttgtgcgcag aggcgagtgc atgcgcgctc
    12841 tcctggccga gaccgtggtg gaaaaacgct ggcgaattct gatccgcgaa ctccagcaga
    12901 tgcgcgccgc ctgaaccctc cccgtcatgc ctcaccccgc cccgccggac gatccgctga
    12961 agaaagcctt cctcggcttt cttgaatccg aacggcgctg ctcgccgcgc acgctggaga
    13021 actaccgcca cgcccttgcg gaattccgcc accgccaaag cggcttcacc gcctggctca
    13081 cactcaccgc ggaggatttt cgcgggtatc ttttcgatca gatgaagcga ggcctggggc
    13141 gcgccaccat ccggctgcac ttcgccgcgc tgcgctcctt ttataagtgg ctgacgcacc
    13201 gccaggggct ggagcgcaat cccctgctcg atgtccagct tcccaagcag gagaaaaaac
    13261 tgcccgtcgt gctcaccgtt ccgcagatca cggcaatgct cgaactcccg ctcaaactcg
    13321 aaaaagacag gcaggcgcca gcctgggcgc ctgagcgcga cgccgccgtg ctcgaactct
    13381 tctacagcac cggcatgagg ctggccgaac tctccgcgct caacgtcgcc gacctcgacg
    13441 cctacagcga caccgtgcgc gtcaccggca aaggctccaa gcagcgcctg ctgcccgtgg
    13501 gcgcgcccgc catggaagcc gtccagcgtt accggcagaa ggcggccgtg catgacgggc
    13561 cgctttttct cagcaaactg cgccgccgca tcaccaccca ggcagtcgcc gacatcgtgg
    13621 acaagtattg gagaaagtcc ggcctgcccg tccacgtcac cccacacaag ctgcgccaca
    13681 gcttcgccac ccacctgctc gacaacggag ccgacctgcg cagcgtgcag gagctgctcg
    13741 gccatgccag cctcagcacc acgcagatct acacccacgt ctccacccag cgcatgaagg
    13801 aggtctatga ggccgcgcat cccagggctt gatccggcgg taattcgatc ttgattgccc
    13861 ttctgatgat ccctcgcctg tttctgctcc tgctgctcct tcccgtgggg ggcgctgctg
    13921 aaaccgtccg cctgctcacg atcggcaaca gtttttcgcg caatgccacc aatcacctgg
    13981 gcgacctcgc caaggccgga ggtcatgagt tgatccacga atccatcgtc gttggcggag
    14041 cctccctgga actgcattct gaaaaggccc ttcttcacga aaaggatccg gcgcacaaag
    14101 acggcctcta cacgagcgga cgcagcctcg cgcagcatct gcggcaggag cgctgggact
    14161 ttgtctccat ccagcaggcc agcatcaaaa gtcatgactt cgccacctat gagccgcatg
    14221 gctcccggct cgccgccctg atccgcagcc atgcgcccgg cgcgcgcatc ctcgtgcatc
    14281 agacctgggc ttaccgggtg gatgacccgc gtttcaccaa gccctcggac acagatggag
    14341 agcccgccac ccaggagcag atgtaccgcg gcctcaccgc ggcctacgac cagctcgcca
    14401 gcctcatcgg cgcgcgagtc ataccggttg gtgacgcctt ttttgccgcc gacaccgacc
    14461 ctcaatgggg ctacaaaccc ggctccgtcc cttttgatcc aaagacagcc cggcgccccg
    14521 ccctgccgga gcagacacac tccctgcatg tgggctggcg ctgggccaaa gcgcgcaaca
    14581 gcgtcaaaat caccctcggc atggacggac accacgccaa cctggcgggt gaatatctcg
    14641 gagcctgtgt ctggtacgag gtgctctttg gcgacactcc gcctggcagc acccacctgc
    14701 ccaaaggact cgatgccgcc cacgcgcgct ttctccagga gaccgcccac cacgccgtgc
    14761 aggcccggct cgcgagcggc caatgagccg gcccgcatcc gcgcgatcag tggatgccgc
    14821 tccctgacga agtggcaaat cacctgcatg cacgggtgag cgacgcgcat tttttgcatt
    14881 ctgccagacg gattcatggc ggaaaccatg cctcggaaaa aatgcggcca tatccgacca
    14941 aagtcggata cccgcttttt cgaaacgggt caaagatgcg cgcggaacca gaacacacac
    15001 ccgacatgaa tacatccgca ccagccaaca tcctccgccg cagccttcca tgcgtgctca
    15061 tcggcatcct catgctttgc ctgccgcggc aggtcgaagc gcaggtgcct ccctacctcc
    15121 ttcgtcattc catccccgcc ccgctggagg cgcaatatgg aggcaatttg ggcttcagct
    15181 gcgctcagag cggaaacctg gccgccctgg gcgcgccgta cgacgacacg ggcgcgcagg
    15241 acgcgggggt agtgaaaatc ttcgacacga ccaccggaga cttggtgcag gtggtgccga
    15301 atccgacgct tggaacttat gggtattttg gcacagcgat cgccatggag ggtgcacggc
    15361 ttgtcgtcgg ggctccaaat cagttcgcag gtgcgagcgg cgctggagcg gtctatgttt
    15421 atgacctcgg cgggccgaca ccgaccacgc ccatattgac gatccccaac ccggcaccgg
    15481 ccagctatga cagctttggc agcgcgctgg cgctctctgg agacagactg gtggtagctg
    15541 cagaatggga cgacgcggga gcagcagatg cgggcagtgc ctatgttttt gatctgggca
    15601 gcggcactcc cgcgactccc gtcttcacac tgagcaaccc caatcctgct gcttctgacc
    15661 ggtttggaat ctcggtggcg gttgacgggg accgtgtcgt cgtgggtgtg ttgtttgcag
    15721 atttgggtgc gagcaatgcg ggcagggctt atgtgtatga tctgggcggc atgactcccg
    15781 gcacaccggt actcagcttg agcaaccccg cgcctacggc tgatgcctat tttggcagat
    15841 cggtgggcat ctccgggcag atcgtcgccg taggagcgca caatgacaga acagggcctc
    15901 aaggcactgg cagcgtgcat gtttataatc tgggatccgg aactcctgag acgcctgttc
    15961 acgcgatttt taatcccggt ccgggaagca acgaagcatt tggtagcact ctggtgctgc
    16021 gtggaaagcg gctgctggtg ggggactttg tcgaaaacac aggggccacc gcctctggga
    16081 ccgcttacct ctacgaactg gccagcggct ctcccacggt gcctgcattc actttcaaca
    16141 accctaatcc tggcgcattt gaccggttct cttggggtat atcactggcg gacgaccagg
    16201 tgctgatcgg cgcttacacg gacgacaaag gaggcattga tgcaggcacc gcctattttt
    16261 acgacctcct gtctggaacc cccacgctgc cggtgcgcga gttcaatgat ccaggctttg
    16321 aagtcctgtc tcaattcggc cagtctgtgg cggtgtcagg cacacttgtg gcggtaggca
    16381 ctggcgagaa agacttgaac attgacggcg ccggccaggt gagaatcttt gacacgagca
    16441 gcccgactcc aaatgtgccg gtgctgacga ttccctctcc gcagcccgcc ttgcgcggca
    16501 ggttcggcgt tgcggtggct ctggatggca cccggctcgt ggtcggtgcc agggatatga
    16561 atggcagcgt cgtctttgca ggcgcagtct atgtttatga tctgggcagc gccacccccg
    16621 gcacacctgt gcaggtcatc cccaatcctg ttccgtcctc ctcaagcaat aggtttggga
    16681 gatccgtggc tctctccggc acgcatgtgg tggtgggcgc accggaggag gatactttgg
    16741 ccgccaacgc cggcagtgct tatgtctttg acctttccag cggcactccc ggcgtgccag
    16801 tgctgacact tgatcatccc actgctggag atttgtttgg cttttcggtg ggcatctctg
    16861 gcacccggat catcgtggga gcccatgaaa atgacactgg cgccagcaac gccggcagcg
    16921 cctatgtgta cgacctcgcc agtggcactc ccgccacacc cgtggcggca ctcaacaacc
    16981 ctactccagc cagcaatgat ttttttgggt gcaccgtcgc cattgaaggc ggccgcgccg
    17041 tggtgggcgc gtatggcgat gacaccggcg ctcaaaacgc cggatccgtc tatgtctatg
    17101 acctcgatgg agcgaccccc acggtgccgc tgaacgtgat caacagcccc gccgtcatct
    17161 ccggcgagga gtttggcagg gcattggcgc tttccggggg caggcttgtc gtgggagctc
    17221 cgggcaacga caccggcgca aacaacacgg gcatcgcgta cgtgtataac cttgcaggcg
    17281 ccagcccgac cgtgccggaa gacactctgg aaaatccagc tcccggcatc agcgacaact
    17341 atggcttctc agtggccatc caccagcaac gcatcgtcgt cggagcgcct ttatccgacc
    17401 tcagtggaat gaatcagggg gcggtgcatg tcttcagccc tccgagcatg gacgccagcc
    17461 ttgcgaatct ggaggtcagc cagggcacgc tcatgcccgg ctttgcccct ggcacgacgg
    17521 cttacacact gtcccttccg ccgggaacca gttcgatgac tttgacaccg acggcggccg
    17581 atgccggcgc ggtcatcacc gtcaatggca gcgccgtggt ctccggccag gcaagcggca
    17641 gcctgccttt gatctttggc gacaacctca tctccatcgt cgtgactgcg gaagacggaa
    17701 cgacccagcg gacccacaca ctgactgtga ccgcgccagg gttcagcaca ctggccttcg
    17761 ccagcaaggt gatccccgtg gccagcaacg cggcaggcag catggcggaa gtgatgattc
    17821 agcgcacggg aaatctggac ggcatggcaa gctgcacgct caccagcgcg gatggcagtg
    17881 ccacggctcc cgcgcactac accgcccaga gcagcctcca ggtggatttt ggcgacggcc
    17941 aggatacaaa gcaggtgcag atacccatcg cggccgatgc ggcagccacc gtggcacggg
    18001 catttaccat cacactggcg gatgccagca ctggtgtggc gctcggacaa ccttccgccg
    18061 ccaccgtggt catcctgcct cccgcagcct tcacggaaaa agtaaagcct gtggtgaaat
    18121 tcacagctcc tgccaataac gccgtcgtgg ttgacacagt gcctgtgaca gtgagcggca
    18181 tggccacgga caacatcggc atccacaaag tgcaggtctc gctgaacaac ggcccctttg
    18241 tggacgccaa cgtggccagc cctggcggca ccagcacgga atggaatttg aatgtgacac
    18301 ccgcgccggg caaaaacacc ctcaaagtcc gctccgtgga tttcaaaggc aacctctcca
    18361 gcgtcatcag ccgcggcttc acgcatctgc gcacgctgac cgtggcgata aatggaccgg
    18421 cgggatcagg cacggtcagc accggcttca tgccgtcatc gaagcgcatc gctgggaaaa
    18481 gctacagcat cacggccact ccaaaacccg gtttcgtgtt tgatggctgg acggccagcg
    18541 acaccgccgg caccggcatc accgtcgcaa gcgcggaact gccaaagctc gctttcatca
    18601 tgcagcccgg cctcagcctt acggcaaagt tcatcgtcaa tccattcgac gcgcagctca
    18661 caggcaccta ccgcggactg gttagacctg atatgcagac tgacgaaaac aattcaaccg
    18721 ccggcctggt tcataatgtc gtggtcacca gcaaaggcgc cttttcaggc agcctcctca
    18781 tggacggagc caagatcaag ctggctggat tttttgacaa cacgggtgat gcccgcttcg
    18841 gccccgcgcg ctcgaagacg ctggtggtga cccgcaaaaa caagcccgtg atgcagatca
    18901 gcctccatct cgacatgaca ggcttcacac accgcatcac agggggtgtg gatcaactcg
    18961 accaaagtgt tcttgtggcc cattcggaca tcaccgccga ccgtgccgcc tttggcaagc
    19021 tgaccgaggc tccagcagct ctggcatcca aaaacggcaa gtcctacaca ctcgttttcg
    19081 ccgccaaaac ccagacgccc gccattccgc tgaacctgta tccgcaaggg cacggccatg
    19141 cctctgtcat catcaaatcc accggtgcgg tgactctttc aggcaagctt gcggacggca
    19201 ccatcttcac ctcggccacc accttgtcga agcatttgca gttccccgcg tttgcccagc
    19261 tctatcaaat gcagggcagc ttcggcggtg agatcactgt cgccgacgca ggccagaacg
    19321 cggacgatct caccggtgtc atgttctggt tccgcccgca agtcaacggc tcgcagtggt
    19381 accaggccgg atggccggat ggcatcggaa cggacgcgct cggcaaccgc tacacagtac
    19441 cctcaggcag cagcgtcttt ccgggcctgc aaaatcttcc cgcgaacgca gacctcagcc
    19501 tctcaggcgg actgctcgcg ccatccattc tccaggacat gctgatcacc ccggccaaca
    19561 aggtctctcc tgtgcccgcg gccgcactgc ccaccgcggt catcaccgcg aagactggcg
    19621 gcatagccgg taaattccgc cacagtctcg gggccagcac cgcctatcag ggagtgattc
    19681 tgcaaaaagg cacccggaca ggcgcgcatg gctggttcct ctcgccaaaa cccgccaagc
    19741 cagacttcct gggcgaaagc ggcgccatgc ggatgacggc gaagtgatca gccaatcaac
    19801 cacacaagca aaacgctctg acatgccatg ccagggcaaa cccggcgcgc ggggaggaaa
    19861 gacacctcgc cgcgcgcccg tttgcctccg ggaatgcccc gccttttttt catcatcacc
    19921 gttctgctct ggaccggccg cgcctctctt cccggtgcgg tcgaagatcc gtcggagttt
    19981 tttgaaaagc gggtccgccc cctgctgttg gagcactgca ccgaatgcca cggcgctgac
    20041 aaacaaaaag gcagcctcag gctcgaccac aaggcgggct gggaacgcgg gggcgactcc
    20101 ggccccgcgc tcgcgccggg gaacctggac gggagcctgc tctggcgcgc ggtcggctac
    20161 gaggatcgcg acctgaaaat gccgcccaaa aagcgcctgc ccgaaagtgc cctggccgac
    20221 ctgaaggcat ggatcaccag cggcgcgcat gacccgcgcg acgcgccccc ggaggcaggc
    20281 ggcggcaagt ctggtccgaa ggccgacggc agcttctggt cgttccagct ccccaaactc
    20341 cacgcgccgc caaccgtcca aaaaaacgac tggcccgcgc gtccgttgga tcacttcatc
    20401 ctcgccaggc tggagcagaa ccaaatgacg cccgcgccag acgcggacgc gcgcacgctg
    20461 gcgcgcaggc tgcactttga cctcaccgga ctgccgccct cgcatgagga ggtggagagc
    20521 ctcgcgcaag gcaaggtcgg ctatgaggaa agagtggacg ccttgctggc ctcccccgcc
    20581 ttcgccgagc gctggacttc ccatttcatg gacatgacgc gctttgccga atcctccggc
    20641 ggcgggcgct ccctgccgtt caaggatgcg tggcgcttcc gcgactatct tctggaaagc
    20701 ctgcgcgacg gcgttcccgt ggaccgcatg atccaggaga tgaccgccgg tgatctgctg
    20761 ccgtttgaaa acgccgccca gcgccggcgc cagatcaccg ccaccggctt cctagctctc
    20821 ggccccacca attatgagga gcaggacaag cagctcctgc gcatggacat cgtggacgag
    20881 cagctcgaca ccatcggcaa atccttcctg ggcatgacca tcggctgcgc gcgctgccat
    20941 gaccacaagt tcgaccccgt ctcgacgcgc gactactacg cgctggccgg catcatgcgc
    21001 agcacccaga ccctcaagaa ctacaccgac aacgtcgccc actggattga cactcccctg
    21061 ccctacgagg gcgaactgga ggagcaaatg cgcgccaagg aggaaaagct cgccgagctg
    21121 aacaagcagc tcgccagcct caaggacgaa ctgcgcgaca tcggcggcgc gaacctgcgc
    21181 aaaaaaaaca gcatcgccgt caccgacctg cccggcatcg tcattgacga cagcgccgcc
    21241 cagagggtcg gcaactggaa ggagtccatg ctggtgaaac ccttcatcgg cgaaggctat
    21301 gtccatgaca aaacgggcga caccggcgag cgcaccattt cattttcccc gaaaatcccc
    21361 gtcaccggcc gctacgaggt gcgcgtggcc tacatcggcg gcccggaccg cgctgaaaaa
    21421 gtgcgcgcgg atgtgctcca cgccgacggc gaggagacgg caagctattc cacccgcacg
    21481 gcgagcatgg agggg
//
LOCUS       DAOMYJ010000076        20963 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Prosthecobacter sp. isolate AMR_MDS_5629
            NODE_888_length_20963_cov_20.108380, whole genome shotgun sequence.
ACCESSION   DAOMYJ010000076 DAOMYJ010000000
VERSION     DAOMYJ010000076.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867850
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prosthecobacter sp. (wastewater metagenome)
  ORGANISM  Prosthecobacter sp.
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Verrucomicrobiaceae; Prosthecobacter.
REFERENCE   1  (bases 1 to 20963)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 20963)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.30
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:34:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,742
            CDSs (total)                      :: 4,684
            Genes (coding)                    :: 4,676
            CDSs (with protein)               :: 4,676
            Genes (RNA)                       :: 58
            tRNAs                             :: 55
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20963
                     /organism="Prosthecobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_888_length_20963_cov_20.108380"
                     /isolate="AMR_MDS_5629"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1965333"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            212..787
                     /locus_tag="PLP58_09890"
     CDS             212..787
                     /locus_tag="PLP58_09890"
                     /inference="COORDINATES: protein motif:HMM:NF018038.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ThuA domain-containing protein"
                     /protein_id="HRJ08870.1"
                     /translation="MAEKTSVTLADEWPSDEQWRSADAVVFYRKGDWSPAQAQQFDTF
                     LQRGGGATFIHWSLEAGDAAPALAHRLGLASQRKVTKYRHGPIQLVFEGQHPIARNFT
                     RAGFTDEIYWNLIPSEKTPVKPLATSEGHPQLWTQEPASGGRIFVTLGGHYSWNFDDP
                     LFRTLVLRGIAWTAKEPVDRFNNMIEAGLEP"
     gene            complement(828..2102)
                     /locus_tag="PLP58_09895"
     CDS             complement(828..2102)
                     /locus_tag="PLP58_09895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009958291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HRJ08871.1"
                     /translation="MNVEDLTAEDAESAEKPQSDSKNESLRASASFALSAVDPNERQR
                     RWRLVLGGEANESCGGLAGEDVEIDQALAALYEPEGSGSLKKQRGGTEKSAPRVARWL
                     GDIRRYFPSSVVQVMQKDALDRLGMREMLLQPEMLEAVQPDVHLVANLISLSGIIPAK
                     TKETARMVVRKVVEELMRKLAEPMRTAVTGALNRAVRNRRPRLAEIDWNRTIRANLRH
                     YQEDYQTIVPETLIGFGRKSQKTQRNIILCIDQSGSMAASVVYSSIFGAVMASLPAVR
                     TQLVVFDTAVVDLTEKLDDPVELLFGTQLGGGTDINKAVGYCQSLITDPTNTILVLIS
                     DLIEGGVEKNLLQRTNELVQSGVQFITLLALSDEGAPCYDKELAAKMSILGVPSFACT
                     PDKFPALMAAAIKKEDISFWAAKEGMNATRGK"
     gene            complement(2099..4360)
                     /locus_tag="PLP58_09900"
     CDS             complement(2099..4360)
                     /locus_tag="PLP58_09900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007413934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5682 family protein"
                     /protein_id="HRJ08872.1"
                     /translation="MTHLFGIRHHGPGCARSLVRALEELSPDCVLIEGPPEAEAVLAA
                     AVHAEIEPPVALLLYCPDDTKKASFYPFAEFSPEWQAIRFGLQRGLPVRFMDLPQVHD
                     LALRDAEVSALLSEESDSPDEVAEDDISGDPLDWLGRAAGYGDGETWWNQMVEERRDS
                     DSAGLFAAIQEAMTSVRAEIPRKRAPEAQHRESLREAHMRKTIRQAEKDGFERIAVIC
                     GAWHVPALATMPSAKSDNDLLKGLPKVKVAATWAPWTYGRLAAGSGYGAGVLSPGWYE
                     FLWRNQAAEASQRSIAWISKVARLMREEDIDCSSAHVIEAARLSDALAAMRERPAAGL
                     DEINESIRAIICMGYDAPLRLVRSRLIIGEKLGRVPPDVPTVPLQQDLEKQQRSLRIK
                     PEALAKDLDLDLRNENDLSRSHLLHRLRLLGLKWGEVKGTGRSAKGTFHEVWQLQWQP
                     EFAVSIIEASRWGHTIADAATALAIDHAEKATSLPELSALVDRVLLANLPHAVEPVTH
                     ALENLAAIAGDASQLLEAIAPLANIFRYGNVRQTDASLVGHVLDGLVTRACISLGGAC
                     ASLDDEAAAAMRKRILATHQAIKLIGKDTHLADWMPALGRLAVLGGTHGLVSGMASRL
                     LFDEQAEDADATEQRMSQALSIGNDPSPAAAWLEGFLHQSGMILLHDDRLWRILDTWL
                     ASLSDDNFTRILPLIRRTFSQFPGTERTQLGARAKHSGSTASAPTAIATEFEWNETRA
                     LKPLPVLEQILGL"
     gene            complement(4357..5124)
                     /locus_tag="PLP58_09905"
     CDS             complement(4357..5124)
                     /locus_tag="PLP58_09905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ08873.1"
                     /translation="MPVESMDDVYRLAQSVDAQTQTIPSQDGSYLYWTSNSGAQLWLQ
                     VTPKREFAGMNPHFSGPARMKVRLTAYIPSTTPSAMDGSYHGWADPVESEAEGSYPFV
                     FDAPDASAHRKRKLPEVVDLQLAAFAHELSAYESSEAYNAAQSSAELQMASESFIPSG
                     LFGEREGTKALGMMTGTILAAERKTNELTGKTFWWALVQSLGGQFDVVADEELVTAPL
                     VVGGVLQGSFWLSGQILTPPPAASSGGIFSRLFGKKS"
     gene            complement(5170..6246)
                     /locus_tag="PLP58_09910"
     CDS             complement(5170..6246)
                     /locus_tag="PLP58_09910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009958294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HRJ08874.1"
                     /translation="MNTLRQHAEQLFAHELDELKKADTRQRPTNWNLSPWAVSTYLLG
                     GKLDNGFEVSAKYIGNRRVIEIAVATLATDRALLLYGVPGTAKSWVSEHLAAAISGDS
                     TRLIQGTAGTSEEQMRYGWNYALLLSKGPSKEALVPSPLMKAMSDGRIARVEELTRIP
                     ADVQDTLITILSEKTLPIPELSSEVQANRGFNVIATANNRDKGVNELSSALKRRFNTV
                     VLPVPATEEEEIAIVSKRVAEMGKSLELPAEPPALKEVRRIVQVFRELRDGTTADGKT
                     KLKQPTGTMSTAEAISVLNSGIALAAHFGDGTLRAGDVAAGLVGAVIKDPVQDAVVWK
                     EYLETVVKERDEWKDLYRACREVI"
     gene            complement(6255..7052)
                     /locus_tag="PLP58_09915"
     CDS             complement(6255..7052)
                     /locus_tag="PLP58_09915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ08875.1"
                     /translation="MKAFLHWLRSRFQPPPASEEAMKPVAKAEPSDQTIPHEHQHTPN
                     DQDRDPRRALGRVRECAAGAGNRRSESAWAAADKAESQHCGLGALSKLEGEELSARTR
                     SLDHRNGAEHDAFFDPATRRVIKFTQPGEFGAWGGLEEYLQRLAWANEFFGDDIQVEG
                     WMHYANEPAPRVVTTQPWYRVNPDRPEPTLNEIDLYMARMGWLKAYDGAWIHREREIV
                     ISDALPKNFVIDVAGYIQPIDLIIIAPDDEQWNRLQNMARNLAQPAV"
     gene            complement(7049..8578)
                     /locus_tag="PLP58_09920"
     CDS             complement(7049..8578)
                     /locus_tag="PLP58_09920"
                     /inference="COORDINATES: protein motif:HMM:NF039502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5691 domain-containing protein"
                     /protein_id="HRJ08876.1"
                     /translation="MNALAPLATSALLGTERRAPDWPMLEGALGTLLSRIPRDSVEKA
                     LLQAAGVLGTCQLAGFLPARAGNVPAPATDDALPEDSRIDLLTSILTDGPERLQAEAF
                     IKIAAAGRQLPHRLLPRALECGRRSVALRPSLLRVLGRRGEWLAAQNDSWAYAVGGGS
                     AETLIDDVWQHGSLDQRRLYLVALRERDAAKARELVREAMSTEGAKERTAFIECLATG
                     LTMDDQDLLESTLTDKSKEARQTAARLLSTLPESRFAQRMAAHLASFVKAEKKLLRGT
                     VVTLEAPNAFDAAWKADLIEESKPKGASMGERAWWLFQIVRSVPLSWWEAQTGMNPAE
                     CLALAQKSDWKDALLQGWAEAQAAQRRVEWAEAFLGISLPPNGPLTVFDLLETLPPTL
                     RETYFLKLVTDNDAKMIALSTIVDRFIKGLPMDAPMLSPAVAVKAVKILKQRIHSGEA
                     RYDWQLRHSLVELACLLPVAAFDDLAKGWELAKEETQPFAEAIARIGIVLDQRKQLQN
                     P"
     gene            complement(8575..9975)
                     /locus_tag="PLP58_09925"
     CDS             complement(8575..9975)
                     /locus_tag="PLP58_09925"
                     /inference="COORDINATES: protein motif:HMM:NF016330.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SWIM zinc finger family protein"
                     /protein_id="HRJ08877.1"
                     /translation="MLATDQILALSPDDSSAKAAKGLLAPAKWPMLGHDEVAIWGECQ
                     GSGSKPYQVIIEVSGPSFKCSCPSRKFPCKHGLALYLLLAQKESAFTANTQPQWVNEW
                     LAARQERAEKKAEKAAQTETKPADPEAAAKREAKRLDRMINGAQDLERWMADLVKHGL
                     ADLPAKPVTFWRDAAARLVDAQASGLAAGVRQLESAVSSGEGWQQRTLLSMGRLQLLT
                     EAMQRMDALPDLLRHDVRSSAGWAMDKEEVLAANDRVADAWHVLGISHDENEKLWERR
                     VWLRGAASGRNALLLDFSHGGRRFDQVFVPGTVVKATLCFFPSASPLRALWVDEPQGA
                     QRAQSYAEDAESNEIPLRTSALSATSAVNPTWDAALESIAEALAGQPWFQRLPLMLSH
                     AIPVFRDLRWYLRDAEGREVQMRMSMDDGWQLCALSVGHPLSIFGEWLGERLRPLSAW
                     HGESTQPCWTEVTSLA"
     gene            10095..11033
                     /locus_tag="PLP58_09930"
     CDS             10095..11033
                     /locus_tag="PLP58_09930"
                     /inference="COORDINATES: protein motif:HMM:NF025465.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PmoA family protein"
                     /protein_id="HRJ08878.1"
                     /translation="MKPASLLFLALALPTFAFDITVTDKDHVAVANGGKVVAKLMMAN
                     DLSTPERHHETYKPYMHVFSADGSTRLTKGAGFNFTHHRGIFLGFSKIGYNGKSYDRW
                     HMKGGDQVVTKVAPGENAFTAHIDWQGDTTAPFLTEERQFSFTTPAKPFYLGIEMNSA
                     IKTVSGEADMNGDPEHAGAQFRPSELVDTKTTTYLFPGENIDAHKVKDLPWAAEIFTV
                     EGKTFTVVILNHPNNPKDTATSAYRDYGRFGMFPKGKASAESPFKLRYQWLIAEGDVR
                     DAAAFQDAWNTFAGKSEPVPPLTVKASEQPKPKAKK"
     gene            complement(11264..12550)
                     /locus_tag="PLP58_09935"
     CDS             complement(11264..12550)
                     /locus_tag="PLP58_09935"
                     /inference="COORDINATES: protein motif:HMM:NF014571.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2172 domain-containing protein"
                     /protein_id="HRJ08879.1"
                     /translation="MSAQVHHYTQADLVAALRGAGARAGDVVFVHASLGRLGYPERGR
                     SMADACATALDALLEAVGPAGTVLVPTYTYSIGKGEIFDPAATPSTLGDFTEHVRQHP
                     AALRSADPMLAVSGIGPEAADLLENLPRTCYGPGSLYERLEQRGAKLVMIGLGLFWAT
                     YRHYIEEKAGVPFRFKKRFTGVVRADGREGKQTWIYSCQIRQPNCAPDGVALEKMARE
                     QGICVSAKIGRGEVCVTHCADYMRLGMAELAENPWLSAKGPPLTLDELIALEDARAGA
                     SPAALRSGFFSEGDLGAALSALSGQAPVGDLVTGMQFGDFIVPEKWTCLEAVIRARDG
                     REMLSCRNDPRHVIPCSQPFEGEVSREELLRHLHVCERLEDGAPTAFLPTERDWGFAC
                     TREQLAAFQEESWQVIIRATAGFGRVSVGAPAKARE"
     gene            complement(12547..13704)
                     /locus_tag="PLP58_09940"
     CDS             complement(12547..13704)
                     /locus_tag="PLP58_09940"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF017026.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HRJ08880.1"
                     /translation="MSQPCPDVIAFPESPATHPDRWVLMNVHTRQSLGAGPEVLEVLG
                     APDTATDKTYRVWKIWRFSHLDGLLADPSRFKRDAATWGDPELVDHTALVKLLTEQCL
                     LIDDLDAYRARFDIKRSLLDRQRFGTYHQELGQQLIAFQRADPAAWWLQQKFTPDLSA
                     IRKDNLYGAVQDRFLDEWLPKRLTPGMRVLDLGCGAGTIAHKMSRLGASVLGVDPNTE
                     YIRLASERAVGDASFEALDLNTPGALDALPAASFDAVFMSDALLFYFVPYEPGKPLDL
                     QRFIADVRWLLKPGGSFWSLEPHPVFYVQPWLGDADRPFTVMTEYLHTRWRINPPLSA
                     LTRPFLESGFVVTGVEEPAARRDDAEVGKRDAAFASEFPVWLLVEFQLAGA"
     gene            complement(13701..13952)
                     /locus_tag="PLP58_09945"
     CDS             complement(13701..13952)
                     /locus_tag="PLP58_09945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0000036 - acyl carrier activity [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HRJ08881.1"
                     /translation="MNVTQTVLDYFASKGGLPGATDAEKLACSYLDAKVIDSMGIIEM
                     ISHFEDVCGVRFEADDMQSEDFQTVGGLIRVIEKLRSQA"
     gene            complement(13984..14646)
                     /locus_tag="PLP58_09950"
     CDS             complement(13984..14646)
                     /locus_tag="PLP58_09950"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF016115.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016812 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in cyclic amides
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclase family protein"
                     /protein_id="HRJ08882.1"
                     /translation="MRIIDLSYPIHEGMTTFPVHWHPVVEITQLGRFGIENRETRKIT
                     MGTHCGTHIDAPRHFIPGGPTIEQIPLEWLVGPARVLDFTPVTQPKQEFGVADFQRLL
                     GDEKPQRLILRFDWSDHWGTMKYYSDQPCITEEAGHWLVERGVRVLAMDTPQVDSPDN
                     GRTGPKDSPLHKIMLAAGVVFVEYCTNLRQLTRSEVELVVLPLNILGSDGAPARVIAI
                     ER"
     gene            complement(14650..15621)
                     /locus_tag="PLP58_09955"
     CDS             complement(14650..15621)
                     /locus_tag="PLP58_09955"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012360.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0120225 - coenzyme A binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HRJ08883.1"
                     /translation="MSAASQPRDDRSLETLAGRFPQLRADRMAWFDEPRENAAPARVL
                     FAEFHGGRVDDNRALEAAISGALAEMGLAADVEIVRAGWLPLRADGRVAHEELGARHA
                     AAVEAKKRACIEDGSFAVLPEEMKDCFKRFGTQSKIRTPCEITFPKCVSIGNWVSFGR
                     YGKIVMLPKEAFGGACGVLARRHYPELAEGFDFTGWDDDRPADLVLGDGTTLGDRYFI
                     ICTLSVEFGKHVMTASDLFVSDCHHIFEEVELPPVLLPPTKGRPVKIGDHVWIGIRCS
                     ILEGVTIGRHAVIAAHSVVKEDVPPYALAAGTPAKVRRFFAPESNDS"
     gene            complement(15630..16463)
                     /locus_tag="PLP58_09960"
     CDS             complement(15630..16463)
                     /locus_tag="PLP58_09960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HRJ08884.1"
                     /translation="MILGLTHAVLGSADMTADRAFFETRGWQPRFVQEGIPVFAGKRP
                     WMSTASQEVALAYLDSPGLPALELIAYSQPPPAGIAAPLQMIFPANRLPEVAEENATP
                     LAGVRARQYAGITCPLWVSDAAADQGCIVHHVRDMEAARRFWVDGFGFQVVEHGMGQG
                     HPACAEQAGRLFPMLPGQPVQNALSLRFPALMPQWRARLLLLPCTDAPAPALLDGPGF
                     RVVSMVSTKFGVDRARIFEQGGARASTGVMEEVIGGKKMLLDLIEGPDGVMVEIFQTL
                     A"
     gene            complement(16460..18031)
                     /locus_tag="PLP58_09965"
     CDS             complement(16460..18031)
                     /locus_tag="PLP58_09965"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I adenylate-forming enzyme family protein"
                     /protein_id="HRJ08885.1"
                     /translation="MPSQTLIQRIRGVFEENRDRVFLTESMTGRSWTYGEFFDWGQKA
                     AALLRAQGVGKGDRVALILANSAEFAAMYFGCLFAGAVAVPVNQALHTDDVRHILEHS
                     GARLLLFSRCTRPLVLQAAEKLSHMRQLRVVLGQETSFHPDDVDEAWSFDQADAAAAP
                     EPMADMDDEDALLILFTSGTTGRPKGVVHCVRSEFGNAVAFNEAMGFGKDHRFLHIWP
                     MAYSSGYLNTLMSPFMAGGSVVLARAFTAQTILAFWKPVVAHEVNTLWLSPTMMASLQ
                     RMDRDAAGAAYSRERIHTVCAGTAPLALKLKKDFEAKYGVECLESYGLSELLIIAANT
                     PSLSRLPDSVGHVLEGVDFATVDDTGARLPEGGEGEILVRTPWAMLGYLDTETGAVEP
                     PPEWFPTGDIGLRRAGGEVFITGRKKDIIIRGGQNLSPRAVRDCLLHHPAVEDAVVVG
                     LPHDFYGEEVAAALRLKPGLALADEQPSILEHCRRHLAQQAVPTLLRQFEEFPSGVTG
                     KILNHEVRRMLAQKA"
     gene            complement(18044..20302)
                     /locus_tag="PLP58_09970"
     CDS             complement(18044..20302)
                     /locus_tag="PLP58_09970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ08886.1"
                     /translation="MSKSFYLHTPVAGLEDAVSTEQGLVILGKSPDPEALLPMDLAVF
                     TARLARKLGVKGRVPPEQAALPYDAAHDNLIFDAPLAGLVTGRRMRFLRGWKDYGSCV
                     KCFNGIGLLGEHVLKELRRLQKAAPAALKADALAFLRSRKTPGREGGAPIFTEEALAA
                     VTDSDIAALDLEALAIWKTYAARPIVCSTSSNMGISLHEALRFMQSTRVELAGRSFTI
                     LNDDEGWLIIWCPDEQADFMNAEKTEHLRALETEQPPLTVLHTYINRKQRDPGALKDA
                     LDSGGYFFPTNPQSADELQNLLANSLADVARERRCTTEELLADEKSLQTLRSLGCAVE
                     GGHVVVRGGVEGGLFGLMTGYLVMLDETLPRGDTQAVSTWNQASIGAALAAAVLADLQ
                     LREPDQLPAATRAELKAVFPAIDAFLSRKQLGKDLPSRIHGVFDLANLQSLAQLLGVV
                     VERHLSGRGTAYVGLGSSSYANGNRCYEILKESLLNNGPFMGKETFHPATHTLNPIAQ
                     ALIYADDMHRAACRPEFAGMSKDERAAFIIKHARKPEPAGAAAMAGYLLSRLDDRTLS
                     IVELAAVLRLAGFDSALFLEFAGFNKDVQGTNWWLQEASEEGPFMETLARQFLQLLDR
                     DLDELCALARKERVHSRLNYRLRPLDPPAFEALNPAVNIYLTGDNTRQPKTRLVLAIL
                     ERYETTRGQLAKWVAEDQEVQNRAQEVDFITLVNVGFSRTASALRWLQEKLDAAGKSI
                     LAARISSLAQKS"
     gene            complement(20312..>20963)
                     /locus_tag="PLP58_09975"
     CDS             complement(20312..>20963)
                     /locus_tag="PLP58_09975"
                     /inference="COORDINATES: protein motif:HMM:NF012434.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ08887.1"
                     /translation="LRAVLEERGLVAQGLAFAVQGYGSAGGHLARVLQKNGLRMVAAS
                     DSTGGLHCADGLDAEALWQAREAGLTFAQMEAPGALRVSAAEVLAVRADLVIPAATAG
                     QITAEMAARLQCRMILEIANGPIRPDAEQAVVERGIEIIPDVVANAGGITMSHFEWAQ
                     NRGGALWTAEEAAARLDTRMIATASRMLQVSRDRKVSPAVAAQLLALEQLSAALAL"
BASE COUNT         3814 a         6675 c         6666 g         3808 t
ORIGIN      
        1 tgacagcgcc cttatcggca gcctcgctaa atgccggcca gccaattaag cccgctcgaa
       61 gtctgaaatc gcgaaaatcc tcgctggagc gcccaatcca ccgcccgcca ccaagccgct
      121 gcatctcctg ctcgtcagcg gaaaaaagga tcacggcctc ggcgagcacg attacccgcg
      181 ctggcggcag gtttggtcac gcctcttcac cttggcggaa aaaaccagcg tcactctcgc
      241 cgatgaatgg cccagcgatg aacaatggcg cagcgccgat gccgtcgtct tttaccgcaa
      301 aggcgactgg tcgcccgcgc aggcgcagca gttcgacacc ttcctccagc gcggcggcgg
      361 agccaccttc atccactgga gcctcgaagc cggcgatgcc gcgcccgctc tcgcccaccg
      421 gctcggcctc gcctcgcagc gcaaggtcac gaagtatcgc cacggcccca tccagctcgt
      481 tttcgaaggc cagcacccca tcgcgcgaaa cttcacccgc gccggcttca ccgatgaaat
      541 ctactggaac ctcatcccct ccgaaaaaac gcccgtgaaa cccctcgcca cctccgaagg
      601 ccatccccag ctctggacgc aggaacccgc cagcggcggc cgcatcttcg tcacactcgg
      661 cggccactac tcctggaact ttgacgatcc actcttccgc accctggtgc tgcgcggcat
      721 cgcctggaca gccaaagaac ccgtggaccg gtttaacaac atgatcgaag cgggtttgga
      781 gccgtgaagt cgcgaaagcg gtcaggaaga cttcgcgtga cggcgcatca cttgccacgc
      841 gtggcattca tgccttcctt cgcggcccag aaggagatgt cttccttttt gatcgcggct
      901 gccatgagtg cgggaaattt atccggtgtg caggcgaagc tggggacgcc gaggatgctc
      961 atcttggcgg cgagttcctt gtcgtagcag ggcgcgcctt cgtcgctcaa agcgagcagc
     1021 gtgatgaact gcacgccgct ctgcacgagt tcgttggtgc gctggaggag gtttttctcg
     1081 acgccgcctt cgatgagatc ggagatgagc acgaggatgg tgttcgtggg gtcggtgatg
     1141 aggctctggc agtagccgac ggctttgttg atgtccgtgc cgccaccgag ttgggtgcca
     1201 aagagcagtt cgacgggatc atcgagcttt tccgtcagat cgacgacggc ggtgtcgaaa
     1261 acgacaagct gggtcctcac agcgggcagc gaggccatga cagcaccgaa gatgctcgaa
     1321 tacacgacgc tggcggccat ggaaccgctt tgatcaatgc agaggatgat gttgcgctgc
     1381 gtcttctgcg atttgcggcc aaatccgatc agtgtctccg gcacgatggt ttgatagtcc
     1441 tcctggtagt gccgcaagtt ggcgcggatg gtgcgattcc aatcaatttc ggccaggcgt
     1501 gggcggcggt ttcgcacggc gcggttcagt gcgccggtga cggcggtgcg catgggttcg
     1561 gcgagcttgc gcatgagttc ctcgaccact ttgcgcacga ccatgcgcgc ggtttccttg
     1621 gtcttggcgg gtataatgcc ggagagcgag atgagattgg cgacgagatg cacgtccggc
     1681 tgcacggctt cgagcatttc gggctggagc agcatttcgc gcatgccgag gcggtcgagc
     1741 gcgtcttttt gcatgacctg cacgacgctg gaggggaagt atctgcggat gtcgcccaac
     1801 cagcgtgcga cacgcggcgc ggacttctcg gtgccgccac gctgcttttt cagactgccg
     1861 gagccttcgg gttcgtaaag cgcggcgaga gcctggtcga tctcgacatc ctcgcccgcg
     1921 agaccgccgc aggattcatt cgcctcgccg ccgaggacga gtcgccagcg gcgttggcgt
     1981 tcgttgggat caaccgcaga gagcgcgaag gacgcagagg ctcgcagaga ttcatttttt
     2041 gaatcgctct gcggcttctc tgcgctctcc gcgtcctctg cggttaaatc ttcgacattc
     2101 ataatccaag aatttgctcc agcacgggca gcggtttcag ggcgcgggtt tcgttccatt
     2161 caaactcggt ggcgatggcg gtgggagcgg aggcggtgga gccgctgtgt ttggctcgcg
     2221 cgccgagctg ggtgcgttcg gtgccgggga actgcgagaa ggtgcggcgg atcaggggca
     2281 ggatgcgcgt gaagttgtcg tcgctgagtg aggcgagcca agtgtcgagt atgcgccaca
     2341 ggcggtcgtc gtggaggagg atcattccgc tttgatggag aaagccttcc agccaagcgg
     2401 cggcaggcga aggatcgttg ccgatggaga gcgcctggct catgcgctgc tcagtggcgt
     2461 cggcgtcctc ggcctgttca tcaaagagaa gccgtgaggc catgccgctg acgaggccgt
     2521 gtgtgccgcc gagcacggcg agccggccca gcgcaggcat ccagtcggcg agatgcgtgt
     2581 ctttgccgat gagtttgatg gcttgatgcg tggcgaggat gcgtttgcgc atcgcggcgg
     2641 cggcttcgtc atcaagtgag gcacaggcgc cgcccaagct gatgcacgca cgggtgacaa
     2701 gtccatccag cacatgcccg acgagcgagg catcggtctg gcgcacgttt ccgtagcgga
     2761 agatgtttgc gagcggcgca atggcctcca aaagctgtga ggcatcgccc gcgatggcgg
     2821 caaggttttc cagcgcatgc gtcacgggct ccaccgcatg cgggaggttc gccaacagca
     2881 cacgatccac caaagcggag agttccggca gtgacgtggc cttttcggcg tgatcaatcg
     2941 ccagcgcggt ggctgcgtcg gcgatggtgt ggccccaacg gctggcctcg atgatggaaa
     3001 cggcgaactc aggctgccac tgaagctgcc agacctcgtg gaaggtgcct ttcgcgctgc
     3061 gaccggtgcc tttgacctca ccccacttga ggccgagcag tcgcagccga tgcagcaggt
     3121 ggctgcggct caaatcgttc tcgttgcgca gatcgaggtc gagatccttg gcgagtgcct
     3181 cgggcttgat tcgcaggctg cgctgctgtt tctccaaatc ctgctgcaag ggcaccgtgg
     3241 gcacgtccgg cggcacacgg cccaattttt cgccgatgat gagtcgcgag cgcacgaggc
     3301 gcagcggggc atcataaccc atgcagatga tggcgcggat ggactcgttg atttcatcga
     3361 gtccggcggc tggacgctca cgcatggccg cgagggcatc gctgagccgc gcggcctcga
     3421 tgacatgcgc ggaggagcag tcgatgtcct cctcacgcat gaggcgggcg actttggaaa
     3481 tccacgcgat ggagcgctgc gaggcctctg cggcttgatt tcgccacagg aactcatacc
     3541 agcccggcga caggacgccc gcgccgtaac cacttcccgc agcgagtcgt ccataagtcc
     3601 acggagccca tgtggcggcc actttgacct tcggcaggcc tttgaggaga tcgttgtcgc
     3661 ttttcgcgct cggcatcgtc gcgagtgctg gcacatgcca ggcaccgcaa atgaccgcga
     3721 tgcgctcaaa gccgtctttt tcggcctgac ggatggtttt gcgcatgtgg gcctcccgca
     3781 ggctttcgcg atgttgggcc tcgggtgctc gtttgcgcgg aatctcggcc cgcacgctcg
     3841 tcatggcctc ctgaatggcg gcgaaaagtc cggcgctgtc gctatcgcgg cgttcttcga
     3901 ccatctggtt ccaccacgtc tcgccatcgc cataaccggc agcacgaccg agccagtcga
     3961 gcggatcgcc ggagatgtcg tcctcagcca cttcgtcagg cgagtccgat tcttctgaaa
     4021 gcagcgctga cacttcagca tctctcaagg ccagatcatg cacctgcggg agatccataa
     4081 aacgcacagg caaaccgcgt tgcagcccga aacgaatcgc ctgccattcc ggtgaaaatt
     4141 ccgcgaaggg atagaatgag gccttctttg tatcgtcggg acagtagagc agcaaagcca
     4201 caggcggctc gatctccgca tgaaccgccg cagcgagcac ggcctccgct tccggtggtc
     4261 cttcgatcag cacacaatcc ggcgacagct cctccagcgc acgaaccaaa gatcgtgcgc
     4321 agccggggcc atgatggcgt atgccgaaga ggtgagtcat gatttcttgc cgaagagtct
     4381 gctgaaaatg ccgcctgacg aagccgctgg aggaggcgtg agtatttggc ctgaaagcca
     4441 gaaggaacct tggagcactc caccgactac caaaggagcg gtcaccagct cttcatcggc
     4501 caccacgtcg aactgcccgc cgagactttg aactagagcc caccaaaagg ttttaccggt
     4561 tagttcattg gtctttcttt ctgctgccaa gatggtgccc gtcatcatcc cgagtgcttt
     4621 ggtgccttca cgttcgccaa acagacccga tggtatgaat gactctgagg ccatttgcag
     4681 ctcggcggag ctttgcgccg cgttgtaggc ttcagaactc tcgtaagccg agagttcatg
     4741 agcaaaggca gccagctgca aatccaccac ttcgggcaat tttcttttcc ggtgagcgga
     4801 ggcgtctgga gcatcaaaaa cgaatggata ggacccttcc gcttcgcttt cgacagggtc
     4861 ggcccacccg tgataactgc catccatggc tgatggtgtg gttgacggaa tataagctgt
     4921 cagccggacc ttcattcgag ccggacccga gaagtgaggg ttcatgccgg caaactcccg
     4981 cttgggagtc acctgaagcc agagttgagc tccactgttc gaagtccagt aaaggtagct
     5041 gccatcttgc gacggtattg tctgcgtctg agcatccacg gactgcgcca gccgatagac
     5101 atcatccatc gactcaactg gcattcccaa agatgaaaag tgacttggca tactggatgg
     5161 agtcgagttc tagatgacct cccggcacgc ccgatagaga tccttccact catcgcgctc
     5221 tttcacgacg gtctcgagat actccttcca cacgacagcg tcctgcacgg ggtctttgat
     5281 cactgcgccg accaatcctg cggcgacatc gcctgcacga agggttccat caccgaagtg
     5341 ggcggcgagg gcgatgccgc tgttgagcac gctgatggcc tcggcggtgc tcatggtgcc
     5401 ggtgggttgc ttgagcttcg ttttgccatc ggcggtggtg ccgtcacgca gctcgcggaa
     5461 gacctggacg atgcggcgca cttctttgag cgcgggtggt tcggcgggaa gttcgagcga
     5521 cttgcccatc tcggccacgc gcttgctcac gatggcgatt tcctcctcct cggtcgcagg
     5581 cacgggcagc acgaccgtgt tgaagcgacg tttgagcgcg ctgctgagtt cgttcacgcc
     5641 tttgtcgcga ttgttcgcgg tggcgatgac gttgaagccg cggttcgcct gcacctcgga
     5701 gctgagttcg ggaatgggca gcgttttctc cgacaagatg gtgatgagcg tgtcctgcac
     5761 atcggccggg atgcgtgtca gctcctccac gcgggcgatg cggccatcgc tcatcgcctt
     5821 catcagcgga ctgggcacga gcgcttcttt ggaggggcct ttggaaagga gcagggcgta
     5881 gttccatccg tagcgcatct gttcctcgct ggtgccggcg gtgccttgga tcaaacgcgt
     5941 gctgtcgccg gagatggcgg cggcgagatg ctcggaaacc cagctcttcg ccgttccggg
     6001 cacgccgtag agcagcagcg cacgatccgt ggccagcgtg gcgacggcga tctcgatcac
     6061 gcgacggttg ccgatgtatt ttgcgctgac ctcgaagccg ttgtcgagct ttccgccgag
     6121 caggtaggtt gaaacagccc acggcgacag gttccagttc gtgggtcgct ggcgcgtgtc
     6181 ggcttttttc agctcatcga gctcatgggc gaagagttgt tcggcgtgtt ggcggagggt
     6241 gttcataagg tggtctagac agcaggttgg gcaaggtttc tggccatgtt ttgcaggcgg
     6301 ttccattgct cgtcatcagg tgcaatgatg atcagatcaa tgggttgaat gtagccagcg
     6361 acatcgatca cgaagttctt cggcaaggcg tcgctgatga cgatctcacg ctcgcgatga
     6421 atccaggcac catcgtaggc tttcagccag cccatgcggg ccatgtagag atcaatttcg
     6481 ttgagcgtcg gctcggggcg atccgggttc acgcgatacc agggctgtgt ggtcaccaca
     6541 cgcggcgcgg gctcgttggc ataatgcatc cagccttcca cctgaatgtc gtcgccaaag
     6601 aactcgttcg cccaggcgag gcgttgcagg tattcctcaa gccctcccca ggcaccgaac
     6661 tcaccgggct gggtgaactt gatcacccga cgcgttgcgg gatcaaagaa ggcatcgtgt
     6721 tctgctccgt ttcgatgatc gaggcttctt gtgcgtgctg agagttcttc accttcaagc
     6781 tttgatagtg ctcccaggcc gcaatgctgg gattcggcct tgtcggctgc ggcccatgcg
     6841 ctctcactcc ggcgattgcc tgctccagcg gcacattcgc ggacgcggcc aagcgctcgg
     6901 cgagggtccc gatcttgatc gttgggcgtg tgctggtgct catgaggaat agtttggtct
     6961 gatggttcag ccttggcaac gggtttcatc gcctcctcgc tggcgggcgg aggctgaaag
     7021 cgagatcgaa gccagtgcag aaatgctttc acgggttttg aagctgtttg cgctggtcga
     7081 ggacgatgcc gatgcgggcg atggcttcag cgaagggttg ggtttcctcc tttgcgagtt
     7141 cccagccctt ggcgagatca tcgaaggcgg ccacgggcag aaggcaggcg agttccacca
     7201 gcgagtggcg gagctgccag tcgtagcgtg cctcgccgct gtgaatgcgc tgtttgagaa
     7261 tcttgacggc tttgaccgcg acagcgggtg agagcatcgg cgcgtccatg ggcagacctt
     7321 tgatgaaacg atccacgatg gtgctgagcg cgatcatctt ggcgtcgttg tctgtcacca
     7381 gcttcagaaa gtacgtttcg cgcaaggtgg gcggcagtgt ttcgagaaga tcgaagacgg
     7441 tgagcggtcc gtttggaggc agcgagatgc cgaggaaggc ctccgcccat tcgacgcggc
     7501 gttgtgcggc ctgggcctcg gcccagcctt gaagcagcgc gtccttccag tcggactttt
     7561 gcgccaatgc gaggcattcg gcgggattca tgccggtttg agcctcccac cacgaaagcg
     7621 gcacactgcg cacgatttga aaaagccacc acgcgcgctc gcccatgctc gccccctttg
     7681 gcttgctctc ttcgatcaaa tcggccttcc acgccgcatc gaaagcattc ggtgcctcaa
     7741 gcgtgacgac cgtgccgcgc aggagttttt tctccgcctt cacaaatgac gcgagatgcg
     7801 cggccatgcg ctgggcgaag cgtgactccg gcaacgtcga gagcaaacgt gccgccgtct
     7861 gccgcgcttc tttgcttttg tccgtgagcg tggattccag cagatcttgg tcgtccatcg
     7921 tgagaccggt ggcgaggcat tcgatgaagg cggtgcgttc cttggctccc tcggtgctca
     7981 tcgcctcacg cacgagttca cgggccttcg ctgcatctcg ctcacgcaaa gcgactagat
     8041 aaagacgtcg ctgatcgagg ctgccgtgct gccacacgtc atcaatgagc gtctccgcgc
     8101 tgccgccacc gacagcataa gcccacgagt cattttgtgc ggcgagccac tcaccgcgtc
     8161 gccccagcac ccggagcaaa ctcggtcgta gcgccacgct gcgtcgccca cattccaaag
     8221 cgcgaggcag caagcgatga ggtaactgtc ggccagcggc ggcgattttg atgaatgcct
     8281 cggcctgcaa acgctccggg ccgtcggtga ggatactcgt gagcaaatcg atccgcgaat
     8341 cttccggcaa agcatcgtcc gtggcaggtg cggggacatt tcccgccctc gctggcagaa
     8401 aacccgcgag ctggcaggtg ccgagcacac ccgcggcttg cagcagagcc ttttccactg
     8461 aatcgcgagg gatgcgagac agcaaggtgc cgagcgcacc ttcaagcatc ggccagtccg
     8521 gcgcacggcg ctccgtgccg agaagggctg aggtggcgag tggggcgagc gcgttcatgc
     8581 gagcgaggtc acctccgtcc agcagggttg agtggattcg ccgtgccacg cgctgagagg
     8641 ccgcagccgc tcaccaagcc actcgccgaa gattgaaagc gggtgcccga cgctgagggc
     8701 gcatagctgc cagccatcgt ccatgctcat gcgcatctgc acctcccgac cttccgcatc
     8761 gcgcagatac catctgagat cgcgaaagac gggaatggca tggctcaaca tcagcggcaa
     8821 ccgctgaaac caaggctggc ctgcaagagc ctcggcaata gattcgagag cagcgtccca
     8881 ggtagggtta accgcagagg tcgcggagag cgcagaggtt cgcaggggga tttcatttga
     8941 ttctgcatcc tctgcgtaac tctgcgccct ctgcgctccc tgcggttcat ccacccaaag
     9001 cgcccgcagc ggcgacgcgc tcgggaaaaa gcaaagcgta gccttcacca ccgtgccggg
     9061 cacgaagacc tgatcaaacc gacgtccacc atgcgaaaag tcgaggagca gcgcattccg
     9121 gcccgaggcc gcgccacgca accacacgcg ccgttcccag agcttttcat tctcatcgtg
     9181 gctgatgccc agcacatgcc acgcatccgc cacgcgatca ttcgcggcca gcacctcctc
     9241 cttgtccatc gcccagccgg cgctgcttcg cacgtcgtgc cgcagcagat cgggcagggc
     9301 atccatacgc tgcatcgcct ccgtgagcag ttgaaggcga cccatgctca aaagggtgcg
     9361 ctgctgccag ccctcgcctg aagacaccgc gctctccaac tggcgcacgc ccgccgccag
     9421 tccgctggcc tgtgcatcca ccagccgcgc ggccgcatcc cgccaaaacg tcaccggctt
     9481 tgccggcaga tccgccagac catgcttcac gaggtccgcc atccagcgct ccagatcctg
     9541 cgcaccgttg atcatgcggt cgagccgctt cgcctcacgt ttcgctgccg cttccgggtc
     9601 ggcaggcttc gtttcggttt gcgcggcctt ttcagccttc ttctccgcac gctcctgacg
     9661 cgccgcgagc cactcgttca cccattgagg ctgcgtgttc gccgtgaacg cgctctcttt
     9721 ctgcgccagc aacaaataaa gcgccaagcc gtgtttgcag gggaacttgc ggctcgggca
     9781 gctgcatttg aacgacggac cgctgacctc gatgatcacc tgatacggct ttgacccgct
     9841 accctggcac tcaccccaga tcgccacctc atcatgcccg agcatcggcc acttcgccgg
     9901 agccagcaat cccttggcgg ctttcgccga agaatcgtcg ggcgaaaggg cgaggatttg
     9961 gtcggtggcg agcattcagc ggacttaaaa acgaatttca tcgcccggca agattctcat
    10021 gccgcgaacc gcattttcac cacctgacat tcgacacaaa gaatgcatgg cgacagcacg
    10081 ttgtcgccca ccccatgaaa cccgcttccc ttctcttcct cgctctcgcc ctgcccacct
    10141 tcgccttcga catcactgtc acggacaagg accacgtcgc tgtcgcgaat ggcggcaagg
    10201 tcgtcgccaa gctcatgatg gcgaatgacc tcagcacgcc ggagaggcat cacgagacct
    10261 acaagcccta catgcacgtc ttcagcgccg atggctccac gcggctgacg aagggcgcgg
    10321 gattcaattt cacgcatcat cgcggcatct tcctcggctt cagcaagatc ggctacaacg
    10381 gcaaatcgta tgaccgctgg cacatgaagg gcggcgacca agttgtgaca aaggtcgcac
    10441 cgggcgaaaa cgccttcacc gcgcacattg actggcaggg cgataccacc gcgccgtttt
    10501 tgaccgagga gcgccagttc agcttcacca cgcccgcgaa gcccttctac ctcggcatcg
    10561 aaatgaacag cgccattaaa accgtgagcg gcgaggccga catgaatggc gatcccgagc
    10621 acgccggtgc gcagttccgc ccgagcgagc tcgtggacac gaaaacgacg acctacctct
    10681 tccccggcga aaacatcgac gcccacaaag tgaaggacct gccctgggcc gcggagatct
    10741 tcaccgtgga aggcaaaacc ttcaccgtcg tgatcctcaa tcatcccaac aacccaaagg
    10801 acaccgccac ctccgcctac cgcgactacg gccgcttcgg catgttcccc aaaggcaagg
    10861 ccagcgccga atcacccttc aaattgcgct accaatggct catcgccgaa ggcgacgtgc
    10921 gggatgccgc cgccttccaa gacgcctgga acaccttcgc cggcaaatcc gagcccgtgc
    10981 cgccgctgac ggtcaaagca tctgagcagc cgaagccgaa agcgaagaaa tgacctcctg
    11041 gcggaggaat gacgaaacca gaatggcgaa tgaatcccga atgtcacaat gatccgaact
    11101 tggtgattcg agcttcctca ttgattcgct tcgttatacc tccgggcttg tttcactcgt
    11161 ctgtctcgct cggctctggt ttcgtcattt ccttcctttg agagttcgcc actccttgcg
    11221 gacacacact ccaaaacgcg gggaacgtaa gccgcgcgga aagtcattcc cgcgctttgg
    11281 caggcgcgcc gaccgaaacg cgcccgaagc cggcggtggc gcggatgatg acttgccagc
    11341 tctcctcctg aaaggcggcc aactgctcgc gggtgcaggc gaatccccaa tcgcgctcgg
    11401 tcggcagaaa ggccgtgggc gcgccatcct ccaggcgctc gcagacatgc aggtggcgca
    11461 gcaactcctc ccggctcacc tcgccttcaa aaggctggga gcatgggatg acatgacgcg
    11521 gatcattccg gcaggaaagc atctcgcgtc cgtccctggc gcggatcacg gcctccaggc
    11581 aggtccattt ttccggcacg atgaagtcgc caaactgcat gccggtcacg aggtctccca
    11641 ccggtgcctg cccggagagc gcggacaggg cggcaccgag atcgccttca gagaagaagc
    11701 cgctgcgcaa agcagcgggc gaggcgccgg cgcgcgcgtc ttccagggcg atgagttcat
    11761 ccagagtgag cggcgggcct ttggcggaga gccagggatt ctccgcaagc tccgccatgc
    11821 cgaggcgcat gtagtccgcg cagtgggtca cgcagacctc gccgcgcccg attttggcgg
    11881 agacgcagat gccctgctcg cgcgccattt tttccagcgc cacgccgtcg ggcgcgcagt
    11941 tcggctggcg aatctggcag gagtaaatcc aggtctgctt cccttcccgg ccgtccgcgc
    12001 ggacgacacc ggtgaagcgc tttttaaagc ggaagggcac gccggctttc tcctcgatgt
    12061 agtggcggta ggtggcccag aacaggccaa gtccgatcat caccagtttg gcaccgcgtt
    12121 gctcaagccg ctcatacaaa ctgccggggc cgtagcaggt gcgcggcaga ttttccagca
    12181 agtccgcggc ctccgggccg atgccgctga ctgcgagcat ggggtccgcc gaacgcagcg
    12241 ccgccggatg ctggcggaca tgctcggtaa agtcgccgag agtggagggc gtggcggcgg
    12301 ggtcgaagat ctcgcccttg ccgatggagt aggtgtaggt gggcacgagc acggtgccgg
    12361 cgggtcccac cgcctccagc agggcgtcga gcgcggtggc gcaggcgtcg gccatgctcc
    12421 tgccgcgctc cgggtagccg agacgtccga ggctggcgtg gacaaaaacg acatcacccg
    12481 cgcgcgcgcc ggcgccgcgg agggcggcga cgaggtccgc ctgagtgtag tgatgcactt
    12541 gcgcgctcat gctcccgcca actgaaactc gacgagcagc cagacgggaa actcgctggc
    12601 aaaagcggcg tcgcgttttc ccacctccgc gtcatcgcgc cgcgcggcgg gctcctcgac
    12661 gccggtgacg acgaagccgg actcgaggaa gggccgggtg agcgcggaga gcggcggatt
    12721 gatgcgccag cgcgtgtgca ggtactcggt catgacggtg aagggccgat ccgcgtcgcc
    12781 aagccagggc tgcacataaa agacgggatg cggctccagg gaccagaagc tgccgccggg
    12841 ttttaaaagc cagcgcacgt cggcgatgaa gcgctgcaaa tccagcggct tgcccggctc
    12901 gtaggggacg aaataaaaga gcagcgcgtc gctcatgaag acagcgtcga aggaggctgc
    12961 gggcagggcg tcgagagcgc ctggcgtgtt gaggtcgagc gcttcaaagc tggcgtcacc
    13021 caccgcgcgc tcggaagcca gccggatgta ctccgtgttc gggtcaactc cgagcacgct
    13081 ggcgccgagg cgcgacattt tgtgggcgat ggtgccggcg ccgcagccga gatcgagcac
    13141 gcgcattcca ggtgtgagcc gtttcggcag ccattcatcg aggaagcggt cctgcacagc
    13201 gccgtagagg ttgtccttgc ggatggcgga aaggtcgggc gtgaactttt gctggagcca
    13261 ccaggcggcg ggatcggcgc gctgaaaggc gatgagttgc tggcccagct cctgatggta
    13321 agtgccgaag cgctggcggt cgagcagact gcgcttgatg tcgaagcgcg cgcggtaggc
    13381 gtcgaggtcg tcaatgagca gacactgctc cgtgagcagc ttcaccagcg cggtgtgatc
    13441 cacgagttcg gggtcacccc aggtggcagc gtcgcgtttg aagcgggacg ggtcggccag
    13501 gaggccgtcg agatgggaga agcgccagat tttccagacg cggtaggtct tgtccgtcgc
    13561 cgtgtccggc gcgccgagca cctccagcac ctcgggtccg gcgccgagag actggcgggt
    13621 gtggacattc atgagcaccc agcggtccgg atgagtcgcc ggcgactccg gaaaggcgat
    13681 gacatccggg cagggctggc tcatgcctgg gagcggagtt tttcgatgac ccggatgagg
    13741 ccgccgacgg tctggaagtc ctccgactgc atgtcgtccg cctcgaagcg gacgccgcag
    13801 acgtcctcaa aatgggagat catctcaatg atgcccatgc tgtctatgac cttggcgtcg
    13861 aggtaggagc aggcgagttt ttcggcgtcg gtggcgccgg gcaggccgcc tttggaggcg
    13921 aagtagtcga ggacggtttg ggtgacgttc atggagtcgg gtcgggagaa aggcggggca
    13981 gtgtcagcgt tcgatggcga tgacgcgggc gggcgcgcca tccgagccga ggatgttcag
    14041 cggcagcacc acgagttcga cctcggacct cgtcaactgg cgcaagttcg tgcagtattc
    14101 aacaaaaacg actcccgcgg cgagcatgat cttgtgcagg ggcgagtcct tcgggccggt
    14161 gcgcccgttg tccgggctgt ccacctgcgg agtgtccatg gccagcacgc gcacgccgcg
    14221 ctccacgagc cagtgtccgg cctcctcggt gatgcagggc tggtcggagt agtacttcat
    14281 ggtgccccag tgatccgacc agtcgaagcg cagaatcaag cgctgcggct tctcatcccc
    14341 gagcaggcgc tggaagtccg ccacgccgaa ttcctgcttg ggttgtgtga cgggggtgaa
    14401 gtccagcacg cgcgccggcc ccaccagcca ttcgagcggg atttgctcga tggtcggccc
    14461 gccggggatg aagtggcgcg gcgcgtcaat gtgcgtgccg cagtgcgtgc ccatggtgat
    14521 cttccttgtc tcgcggttct cgatgccgaa gcggccgagc tgggtgatct cgaccaccgg
    14581 atgccagtgc accggaaagg tggtcatgcc ttcgtgaatg gggtagctga ggtcaatgat
    14641 gcgcatggtt taggagtcgt tggattcggg ggcgaaaaag cggcggactt tggcgggggt
    14701 gccggcggcc agggcgtaag ggggcacatc ctccttcacc acagagtgcg cggcgatgac
    14761 ggcgtggcgg ccaatcgtga cgccttccag aatggagcag cggatgccga tccagacatg
    14821 gtcgccgatt ttcaccggcc tgcccttcgt gggcggcagc agcaccggcg gcagctccac
    14881 ctcctcgaag atgtggtggc agtcggagac gaagaggtca gaggctgtca tgacatgctt
    14941 cccgaactcc accgacagcg tgcagatgat gaaatagcgg tcgcccagcg tggtgccgtc
    15001 cccaagcacg agatcggcag gccggtcgtc atcccagccg gtgaaatcaa agccttccgc
    15061 cagttcggga taatgcctgc gggccagcac gccgcaggct ccgccaaagg cctccttcgg
    15121 cagcatgacg atctttccgt agcggccgaa gctgacccag ttgccaatgc tgacgcattt
    15181 gggaaaggtg atctcgcagg gcgtgcggat cttggactgg gtgccgaagc gcttgaagca
    15241 gtccttcatt tcctcgggca gcacggcaaa ggagccgtcc tcaatgcagg cgcgtttttt
    15301 cgcctccacg gccgccgcgt ggcgcgcgcc cagctcctca tgcgcgacac gcccgtcagc
    15361 gcgcaaaggc agccatcccg cgcgcacgat ctccacatcc gccgcaagcc ccatctcggc
    15421 gagcgctccg ctgatggcgg cttccagcgc gcggttgtca tccacgcgcc cgccgtggaa
    15481 ctcggcgaac aggacccgcg cgggcgcggc attttcacgg ggctcgtcga accatgccat
    15541 gcgatccgcg cgcagttgcg gaaagcgccc ggccagtgtc tccaggctcc tgtcatcccg
    15601 tggttggctg gctgcgctca tggctggcat taggcgagag tctggaagat ttcaaccatc
    15661 accccatccg gtccctcgat caaatccagc agcatttttt tgccgccgat gacttcctcc
    15721 atgacaccgg tggaggcgcg cgcgccgccc tgctcaaaga tgcgcgcccg gtccaccccg
    15781 aacttcgtgc tcaccatcga gaccacgcgg aatccgggac cgtccagcaa ggccggggcc
    15841 ggcgcgtccg tgcatggcag caaaagcaaa cgcgcgcgcc attgcggcat cagagccggg
    15901 aagcggagcg agagcgcgtt ttgcacgggc tgcccgggca gcatggggaa caggcgtccc
    15961 gcctgttcgg cacaggcagg atgcccttgc cccatgccgt gctcaacgac ttgaaagcca
    16021 aacccatcca cccaaaagcg gcgcgcggct tccatgtcgc gcacatggtg tacgatgcat
    16081 ccctggtccg ccgccgcgtc cgacacccac aacgggcagg tgatgcccgc atactgccgc
    16141 gcgcgcacac cggccagcgg cgtggcattt tcctccgcaa cctcaggcag acgattcgcc
    16201 gggaagatca tctgcaaagg cgcggcgatg cccgccggcg gcggctgcga gtaggcgatc
    16261 aactccaaag cgggcaggcc ggggctatcg aggtaggcga gcgccacttc ctgcgaggcc
    16321 gtgctcatcc agggcctctt gcccgcgaag accggaatgc cctcctggac aaagcgcggc
    16381 tgccagccgc gcgtctcaaa gaatgcccgg tccgccgtca tgtcggcgct gcccagcacg
    16441 gcgtgagtca ggccgaggat catgcctttt gagccagcat gcgcctgacc tcatggttca
    16501 atatcttgcc cgtgacaccg ctcggaaact cctcgaactg gcgcagcagg gtgggcaccg
    16561 cctgctgcgc gagatgcctg cggcaatgct caaggatgga gggctgctcg tcggcaagcg
    16621 ccaggcccgg cttcaagcgc agtgccgccg ccacctcctc gccgtaaaag tcatgcggca
    16681 gaccgacaac caccgcgtcc tcgacggccg ggtggtgcag caggcagtcg cgcacggcgc
    16741 gcgggctgag gttctgcccg ccgcgaatga tgatgtcctt tttccgtccg gtgatgaaga
    16801 cctcgccgcc cgcgcgtcgc aggccaatgt cgcccgtcgg gaaccactca ggcggaggct
    16861 caaccgcgcc ggtttcagtg tcgagatagc cgagcatcgc ccaaggggtg cgcacgagga
    16921 tttccccctc gccgccctcc ggcaggcgcg cgcccgtgtc gtccacggtg gcaaaatcca
    16981 cgccctccag cacatgcccc accgagtcgg ggaggcgtga tagggagggt gtgttggcgg
    17041 cgatgatgag aagctcggac aagccgtagc tctccagaca ctcgactccg tatttggcct
    17101 cgaagtcctt ctttaatttc agcgccagcg gcgcggtgcc ggcgcagacg gtgtggatgc
    17161 gctcgcggga ataggcggcg cccgcggcgt cgcggtccat gcgctggagg gaggccatca
    17221 tggtcggcga gagccagagc gtgttgacct cgtgggcgac gaccggcttc cagaaagcca
    17281 ggatcgtctg cgcggtgaag gcgcgcgcca gcaccacgct gccgcccgcc atgaaggggg
    17341 acatcagcgt gttcaggtag ccgctgctgt aggccatggg ccagatgtgg agaaagcggt
    17401 ggtccttgcc aaagcccatg gcttcattga aagccaccgc gttgccaaac tccgagcgca
    17461 cgcaatgcac cacgcccttg ggccgcccgg tggtgcccga ggtgaaaaga atgagcagcg
    17521 cgtcctcgtc gtccatgtcc gccatcggct cgggcgcggc ggcggcgtcc gcctggtcga
    17581 agctccacgc ctcatccacg tcatccggat gaaagcttgt ctcctggcca agcacgacgc
    17641 ggagctggcg catgtgcgaa agcttctcgg cggcctgcaa aaccagcggg cgcgtgcagc
    17701 gcgaaaagag cagcaggcgc gcgccgctgt gctccaggat gtgccgcaca tcatccgtgt
    17761 gcagcgcctg gttcaccggc actgccacag caccggcaaa aaggcagcca aaatacatcg
    17821 ccgcgaactc cgccgaattc gccagaatga gcgccacgcg gtcgcccttg ccaacgccct
    17881 gcgcgcgcag cagcgcggcg gctttttggc cccagtcgaa aaactcgccg taggtccacg
    17941 agcggccggt catggactcg gtcaaaaaca cccggtcccg gttttcttca aagacaccgc
    18001 ggatgcgctg gatgagggtt tgcgaaggca tgggcgggcg ttctcaactt ttctgcgcca
    18061 gcgacgagat gcgcgcggcg aggatgcttt tcccggcggc gtccagcttt tcctggagcc
    18121 atctcaaagc cgaggccgtc cggctgaagc ccacgttcac cagtgtgatg aagtccacct
    18181 cctgcgcgcg gttttgcacc tcctggtcct cggccaccca cttcgcgagc tggccgcgcg
    18241 tggtctcgta acgctccagg atggcgagca cgaggcgtgt cttcggctgg cgcgtgttgt
    18301 cgccggtcag ataaatgttc accgccggat tcagcgcctc gaaggcgggc ggatcgagcg
    18361 gacgcaggcg gtagttcaga cggctgtgga cgcgctcctt gcgcgccagg gcgcagagtt
    18421 cgtccaggtc gcggtccagc agttgcagga actgccgggc cagcgtctcc atgaacggcc
    18481 cctcctccga ggcctcctgg agccaccagt tggtgccctg cacatctttg ttaaacccgg
    18541 cgaactccag aaacagcgcc gagtcaaagc ccgccagccg cagcaccgcc gccagctcga
    18601 cgatggagag cgtgcggtcg tccagacgcg agagcagata gcccgccatg gccgccgcgc
    18661 ccgccggttc cggcttgcgc gcgtgtttga tgatgaaagc cgcgcgctca tctttgctca
    18721 taccggcgaa ctcgggccgg cacgcggcgc gatgcatgtc atccgcgtag atgagcgcct
    18781 gggcgatggg gttgagcgtg tgcgtggccg ggtgaaaggt ctccttgccc atgaaagggc
    18841 cgttgttcag caggctctcc ttgaggatct cgtagcagcg gttgccattc gcatacgagc
    18901 tggagccgag gcccacatag gccgtgccgc gcccggagag gtgccgctcc acgaccacgc
    18961 cgaggagctg cgccagcgat tgcagattcg ccaggtcaaa gacgccgtga atgcgtgacg
    19021 gcaggtcctt gccgagctgc ttgcggctta gaaaggcgtc aatggcgggg aacaccgcct
    19081 tcaactcggc gcgtgtcgcg gcgggcagtt gatcgggctc gcgcagttgc aggtcggcca
    19141 gcaccgccgc ggccagagcc gcgccgatgg aggcctggtt ccaggtgctg acggcctgcg
    19201 tgtcgccgcg cggcagtgtc tcgtccagca tcaccaggta gcccgtcatc aggccgaaaa
    19261 gccccccttc cacgccgccg cgcaccacca catgaccgcc ctcgaccgcg cagcccaggg
    19321 agcgcagggt ttgcaggctc ttttcatccg ccagcagctc ctcggtggtg cagcggcgtt
    19381 cgcgcgcaac gtccgccagc gagttggcca gcaggttttg cagttcgtcg gcgctttgcg
    19441 gattggtggg aaaaaaataa ccgccgctgt ccagcgcgtc cttgagcgct cccgggtcgc
    19501 gttgcttgcg attgatgtag gtgtgcagca cggtcagagg cggctgctcg gtctccagcg
    19561 cgcgcagatg ctcggtcttc tccgcgttca tgaagtccgc ctgctcgtcc gggcaccaga
    19621 tgatgagcca gccctcgtcg tcattgagaa tggtgaaaga ccgccccgcc agctccacac
    19681 gcgtgctttg catgaagcgc agcgcctcgt gcagggagat gcccatgttg gaggacgtgg
    19741 agcagacgat gggccgcgcc gcgtaggttt tccagatcgc cagcgcctca aggtccagcg
    19801 cggcaatgtc ggaatccgtg acagcagcca gcgcctcctc ggtgaaaatg ggcgcgccgc
    19861 cttccctgcc cggagttttg cgcgaacgca gaaaggccag cgcgtccgcc ttcagcgcgg
    19921 cgggcgccgc cttttgcaga cggcgcagct ccttcagcac atgctcgccg agcaggccga
    19981 tgccgttgaa gcacttcacg caactgccgt agtccttcca gccgcgcaga aaacgcatcc
    20041 ggcgtcccgt caccagcccg gccagcggcg cgtcgaagat cagattgtca tgcgccgcgt
    20101 cgtaaggcag cgccgcctgc tccggcggca cgcgcccttt gacacccagc ttgcgcgcca
    20161 gccgcgcggt gaagacggcc aggtccatcg gcagcagcgc ctcgggatcg ggactcttgc
    20221 ccaggatgac caaaccctgc tcggtggata cggcgtcttc gagaccggcg accggtgtgt
    20281 gcagataaaa cgacttcgac atggatgagg attagagggc gagagcggcg gaaagctgtt
    20341 ccagggcgag caactgcgcc gccacggcgg gggagacctt ccggtcgcgc gaaacttgga
    20401 gcattcttga cgcggtggca atcattcgcg tgtcaaggcg cgcggccgcc tcctccgccg
    20461 tccacagcgc gccgccgcgg ttctgcgccc attcgaagtg gctcatcgtg atgccgcccg
    20521 cgttggcaac cacgtcggga atgatctcaa tgccgcgctc aacgaccgcc tgctccgcgt
    20581 ccgggcggat ggggccgttg gcgatttcca aaatcatccg gcattgcagc cgcgccgcca
    20641 tctccgccgt gatctggcct gccgtggccg ccgggatcac caggtccgcc ctcaccgcaa
    20701 ggacctcggc cgcggacacg cgcaacgcgc ccggcgcttc catttgagca aaagtcagcc
    20761 ccgcctcgcg cgcctgccag agcgcctccg cgtccagccc gtccgcgcag tgcagaccgc
    20821 ccgtcgaatc actcgccgcc accatgcgca gcccgttctt ttgcagcacc cgcgccaggt
    20881 gcccccccgc gctgccgtag ccctgcacgg caaaggccag cccctgcgcc acgagtccgc
    20941 gctcctccag caccgcgcgc agc
//
LOCUS       DAOMYJ010000077        20672 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Prosthecobacter sp. isolate AMR_MDS_5629
            NODE_902_length_20672_cov_21.028229, whole genome shotgun sequence.
ACCESSION   DAOMYJ010000077 DAOMYJ010000000
VERSION     DAOMYJ010000077.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867850
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prosthecobacter sp. (wastewater metagenome)
  ORGANISM  Prosthecobacter sp.
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Verrucomicrobiaceae; Prosthecobacter.
REFERENCE   1  (bases 1 to 20672)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 20672)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.30
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:34:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,742
            CDSs (total)                      :: 4,684
            Genes (coding)                    :: 4,676
            CDSs (with protein)               :: 4,676
            Genes (RNA)                       :: 58
            tRNAs                             :: 55
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20672
                     /organism="Prosthecobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_902_length_20672_cov_21.028229"
                     /isolate="AMR_MDS_5629"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1965333"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1842)
                     /locus_tag="PLP58_09980"
     CDS             complement(<1..1842)
                     /locus_tag="PLP58_09980"
                     /inference="COORDINATES: protein motif:HMM:NF019350.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HRJ08888.1"
                     /translation="MMRCLVFTLAAATLAQAGPPAWSERLAFWSRAELGRLDARVSAV
                     DAELSTLAEMALVNSCIRVGLKTGYTTAEDVRWIELTLPEPALADAVVLVPPLAKGAS
                     AVVAGYGFPIRFKLEVFDERDAPFTILDRTAEDFPNPGCYPVVARFEPRPVKRVRLTA
                     TDPWTVDGPEVLALAEMLVLSGTRNAALDAQVGSSSSRNSPRAWTRSNLTDMVTPLGL
                     PVAPQAGGTHGFHSAVAAKADEEKWLTLELPEMTALDEIRLVPARVREVPLWFDYGFP
                     ALYKVEAATRADFSDAVLLHEVTDRLHPAPGANLVCIPARGTRAKFIRVTARQLWYRR
                     SDYVFALAEMQALRGGENLAPRGTFTASDEAREAEGKEWSLSALNDGLTEDGRLIELP
                     EWFAHLEERRALEAERARLVEERETLARRAQQQLVYGSVSGMGAAALALGLMFWRQRR
                     QRRLDAQRLQDKLARDLHDEIGSNLGSITLICSMAAQPGTSAGALREDLGEIGRVAAE
                     TADSMRDMVDLLRTRRVEHEHDWLDVLHRLTERLLRGLALDCALPAAPLTHDPDPETR
                     REIYLFCKEVLHNTARHARATRARFHLTPTREGLRVEISDNGAGFDPA"
     gene            1961..4390
                     /locus_tag="PLP58_09985"
     CDS             1961..4390
                     /locus_tag="PLP58_09985"
                     /inference="COORDINATES: protein motif:HMM:NF037510.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HRJ08889.1"
                     /translation="MPPAAPHAPWTLEGRAPYGEADTRAKLIDPLLYAAGWTEEHIRR
                     EETAGGIEVIEGEARRLSKGRVDYTLRFKLSASVQPVALAYIEAKAETKDPAEGLQQG
                     IDYSGASKRFNVPFVFASNGHAFIEYDHFTGQVSPVRPMSDFPTPEALRQRYEIGKGF
                     SLDSEAARPLLIPYAGGEAVRRYYQDAAIRAVLEALARGLLHVLLSLATGAGKTFIAV
                     NLLKRIRDAGLLRRALFVCDRDELRSQALGAFRNLFGSDVAEVYEGADGKNNARNAVI
                     HIATYQTLAGDQGREARNFFARHYGAKNHFSHIIIDECHRSAWGDWKFILEDNPDAVH
                     IGLTATPRQLILGKKASAASRDEAQWRDNVEYFGEPVYEYTLSQGIEDGYLAPPEIFT
                     FDLFHEGQTEAERLRNVTRAELEAKKLKSSATGAPITSANLKEEYSPSELDERLLLPE
                     RVLALSQHLLEQLIAHGGTPEQKTILFCASDRHAEAMSEQLGNLYRDWCLAHGQEPKA
                     YFAFKCTAKSGGTDHVPDLRGSASSHFIACTVDLLSTGVDVPCVQNIAFMQYVRSPIV
                     FAQMLGRGTRLDPDSGKMMFRVFDYTEATALLGRDFLTKFKGGASRQKQKPPPAEPPA
                     VVEGVMIRIEPTGRYLTATVEGRHARVTLEQYREHIAQRLIAQAGSIADFRRLWVLPP
                     ERRSLIDSIVRGGFSPRALQVAEEAAECDLYDVLGEVGYGIVRQTRADRAYAFTYKQQ
                     PWLQLMPPPTAATVKAIANQFSLGGTEALETPHIFDAPMVVKAGGIPALRLFDEPAKL
                     LTGTKERLFAA"
     gene            4504..5610
                     /locus_tag="PLP58_09990"
     CDS             4504..5610
                     /locus_tag="PLP58_09990"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF013579.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HRJ08890.1"
                     /translation="MNVRMNGFSRDGLAFIDEEQDSSMSGTRVQPADVLLNITGASIG
                     RVCIAPDEICPANVNQHVCIIRSNGRLEPEWIAFFISQPDFQSQILGQQAGATRQALT
                     KEMIEQFELPAPDLPTQRRLATRLKSQMATVEETRRRVEEQFKHCETLRLKFSAEAFA
                     PEVLFQWEERPLLEMSKIVSGVTLGGPTAKEPTRKVPYLRVANVKDGWLMLDDVKEID
                     ATEREIGQFSLKHGDVVLTEGGDPDKLGRGTVWREEIGLCLHQNHIFRVRFDQDQVCP
                     EFAAYQFASKYGKAYFLAHAKQTTGIASINKGVLSRFPFRLPPLKTQRQIAARLREQF
                     THLRQLQNALRTQLEALDKLPGAFLREAFGSLAG"
     gene            5685..6461
                     /locus_tag="PLP58_09995"
     CDS             5685..6461
                     /locus_tag="PLP58_09995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ08891.1"
                     /translation="MQALPSGSEALLVETDAGAAYAKLPGGKEGPHILACELVGTRLA
                     ALLGLPVLDWVLMPYPGQPALKLRSGRMAQAGTAWLTRKVNGFGWGREASDLKHISNR
                     ADIAKLVILDQWTRNCDRYRPEPKLRVNENNVFLNREGAPIGQFILMAMDHTHIFTCG
                     GPLMRDMAKIDLVKDVTAFGLFPEFEGAIQRADAVAAAAALAAVTDEAIHEIVASIPA
                     DWEVDGTIRAAMEDFLRQRRDWLSKTFVSRLFPQDELALV"
     gene            6477..7286
                     /locus_tag="PLP58_10000"
     CDS             6477..7286
                     /locus_tag="PLP58_10000"
                     /inference="COORDINATES: protein motif:HMM:NF022681.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3037 domain-containing protein"
                     /protein_id="HRJ08892.1"
                     /translation="MNTPTTGYYSLIQYCPDRMRLEAANIGVLLFCPERKFLQARLAS
                     SNRRIDRFFGAEAGDLKQINAMKKILEHRLAAESQKIQDVEGLQHFAELLANEIVITE
                     PRAWLVEEPEVELAHLYEQLVGRFIKSEPGPEKLLLEKLRLQLETPRIQAVLKRDVVV
                     KVPVFGNELKVPYAYQNGRLNLIEAHEFNQQREQDIISDVLTKAAQGNLIYKHPDPEA
                     GERQLVVVASFGRAAVEHRQQVETVLRDHHVTFVPDTGLADLERQIAETAH"
     gene            7371..9086
                     /locus_tag="PLP58_10005"
     CDS             7371..9086
                     /locus_tag="PLP58_10005"
                     /inference="COORDINATES: protein motif:HMM:NF014442.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006306 - DNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-6 DNA methylase"
                     /protein_id="HRJ08893.1"
                     /translation="MPKPAKPKPKTTTRSGLDSAIWGVCDILRRSNCAGALQYVPELT
                     WILFLRILDETEEIEAMKAAVVGAEFRPSLEAPFRWRDWAAPEAPERKKLVESTTNAF
                     YDFVNTRLIPHLKALRDKPNATPRQKVMSEIMSSVDRTRVDTQRNLLDVLDKIHEISL
                     DTVDDTHVFALSQIYEGLLLKMGEKGNDGGQFFTPREVIRAIVEVIAPKVGETVCDPC
                     CGTGGFLAQAYEFMRGQLGENATGDQIETLKHKTFYGREKENLVYPIALANLVLHGID
                     EPHLWHGNTLTREEIYGGLYQNAPGQFEVIVTNPPFGGKEGEDAQTRFAYKSRATEVL
                     FLQDVMETLKTGGRCAIVLPEGILFQTNQDAFVKTKRRLLDTCDLWCVLSLPGGVFTA
                     TGAGVKTNVLFFTKGRPTEKIWYFDLSDVKVAKKTPFTRQQLAEFFKLLPDRADSERS
                     WTLDIAKRRRDAKTEADKLRATTAEPKARLKKHQEQLDLLKKAKAKPGKLEEVKKLIT
                     ACEREIREIESKAQTIEDAAFDLKAVNPNAKSTEDTRSSAELMDLIETKGMEVLELLG
                     TLRSL"
     gene            9155..10021
                     /locus_tag="PLP58_10010"
     CDS             9155..10021
                     /locus_tag="PLP58_10010"
                     /inference="COORDINATES: protein motif:HMM:NF021354.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BPL-N domain-containing protein"
                     /protein_id="HRJ08894.1"
                     /translation="MKVTSLFAFLILAVAAHGQTADAPAEKKAPVSSTKVIPPDINGY
                     KTIIAPPENPAPLKIAIFEGKGAPGGGIENVCAKVELMPGSTITRLKPEEIASGGLKG
                     YDVVVFSGGSGSAQAASLGDEGREKVREFVKDGGGYVGICAGAYLACSNFSWGLGILN
                     ASTVSSKWMRGSGYMDAEVTVDGAPILGPVEGVFKVRYNNGPIIKPGTRADLPAYRPL
                     ALFRSEVANNGTPVGVMVNSPAQAVSTFGKGRVFISSPHPENTPGLEHLIPRGIYWAA
                     GKLAEKPLVHVP"
     gene            10018..10170
                     /locus_tag="PLP58_10015"
     CDS             10018..10170
                     /locus_tag="PLP58_10015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ08895.1"
                     /translation="MRAGIHIAALLCALVAARADDDRAACKTPCTVEFSFKPGGAGAS
                     PRAAMC"
     gene            complement(10377..11063)
                     /locus_tag="PLP58_10020"
     CDS             complement(10377..11063)
                     /locus_tag="PLP58_10020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ08896.1"
                     /translation="MPAALPCHTPPAPPRIAMSTIKAAVSFKNHTAAKLSPAAQVIHD
                     KMTLNAGIFDAPPVDMATFQERITDYKARLVARASLARADVMALKAARDLLEETLNSL
                     GNYVNVVAKGDGGIVEKSGFPFYEVNRAPDTTPPGAPANLRLRHSGLPGGFIARYKPR
                     KPNSTNEVQTCAGDPNNEADWVQKGIIKGGRAEITGFPPGAVVWARVRTLGIKNIKGV
                     WSDPAQIRIL"
     gene            complement(11624..12097)
                     /locus_tag="PLP58_10025"
     CDS             complement(11624..12097)
                     /locus_tag="PLP58_10025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ08897.1"
                     /translation="MRVLLKRLVIVAVLAVVIGVIAYQLLPPSEVGRIAVAQLAKADR
                     FSFGGVGVAGSIPEREDWFFAILSSRHSARLFYDLFEQSTTTEARLYALAGLHLTDSA
                     SFRDCAARFAKEGSRVQTQGGCIASDCTAAEAVAAVERGDVERYLKLRRENVRTR"
     gene            complement(13667..15946)
                     /locus_tag="PLP58_10030"
     CDS             complement(13667..15946)
                     /locus_tag="PLP58_10030"
                     /inference="COORDINATES: protein motif:HMM:NF013109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015116 - sulfate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SulP family inorganic anion transporter"
                     /protein_id="HRJ08898.1"
                     /translation="MPHNKAPLHATLPKDLAASVVLFLVALPLCMGVAVASGSPPISG
                     ILAGIIGGIVVGALSGSHKSVSGPAAGLISITISSMAVLGSFESFLLAVLVAGVIQVL
                     LGVLRAGFVAVFFPGSVVKGLLAGIGVILILKQIPHLLGHDADPMGDKSFFQPDNENT
                     FSELFLSLIDIHPGAALVGLFSIVLVLVWDRVASLKKFPVPSAVVVIVLAVLANALFR
                     SIGGSLVIEPSHLVQVPVAGSIGDFFSMFRSPDFSQLSNPAIYTTALLIAAVASLETL
                     LNLDAVDKIDPDQRRAPPNRELVAQGAGNIACGLLGGLPLTSVIVRSSVNINAGARTK
                     LSAITQGFLLLLCVMFLPGLLNEIPLAAVAALLFMTGLRLASPALLRQMWSEGRNQFA
                     PFIITVIAIVLTDPLRGILIGLGVSVLFLLHSNLRRPLRRIRENHVGGEVLRIELANQ
                     VSFLSRPALEKTLFEVPRGGHVLLDARDTDYIDPDVLDLIADFRKDTASAHGVQVSLV
                     GFKDRYHLEDRIQFVDYSTREVQSTLTPDRVLQILKEGNERFLNKQTLIRDHSRVVNA
                     TSAGQFPMAAVLGCIDSRAPAEVVFDLGLGDVFSARVAGNIATPELLGSLEYACAVVG
                     SKLVVVLGHTSCGAVNAAVDLLAAAKKASEATPCGNLDGLVTEIQQAVDTSRLKKAGE
                     WASPAEKAAFANEMARLNVVRTIKVIRQRSATLDKLVNEGKIAIVGALYDVGTGKVDF
                     FQTPESSVQPLPLIIAAAV"
     gene            16057..16971
                     /locus_tag="PLP58_10035"
     CDS             16057..16971
                     /locus_tag="PLP58_10035"
                     /inference="COORDINATES: protein motif:HMM:NF015431.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HRJ08899.1"
                     /translation="MQSAELNYHHLRLFWEAARSGGLREAAERLDLSQPTLSAQIKTL
                     EESLGEKLFERSGRGLKLTAKGRQVMDHAGRIFALGGEMIRSLSEGGSAMSPCLNLGV
                     TQSLPKLVSWRLISKAVHEMPGLHFTCHEGQAADLVGQLVSGRLDVVLADESAPASLR
                     VRAFDRVVDRSRVVFCAAKPLARRWKADFPKSLNQAPVVLPSMHSAWRRELDRWFESK
                     KITPRIAAEFDDSALMKTAAADGLGIVPIASSVLEEAVDRYDLTPFGPPVQCCFESHL
                     ITLEKTLRHPALAALTAAVASGGKVMPV"
     gene            17013..18494
                     /locus_tag="PLP58_10040"
     CDS             17013..18494
                     /locus_tag="PLP58_10040"
                     /inference="COORDINATES: protein motif:HMM:NF013109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015116 - sulfate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SulP family inorganic anion transporter"
                     /protein_id="HRJ08900.1"
                     /translation="MNLRRDLPASLVVFLVAVPLSLGIALASGAPIIAGLIGAVAGGI
                     VTGLLAGSPLQVSGPAAGLTVVVAGLVHQFGWETMCLITACAGVVQLILGFLKVARGT
                     LVIAPAVVHGMLAGIGISIALAQIHVVFGGSPASSPIANVLAFPRQLAAMSGEAAILG
                     FLTIAILIVWKKLPVPALKAVPAPLVAVLVATVVSVIFKMDVARVDLPEKFELTQLKP
                     SSDWGAFIIAVLTVAIIASIESLLCAVATDKLHSGERSKLDKELSAQGAGNLVSGLLG
                     GLPITGVIVRSSANIMAGAASRWSAILHGVWILLFALFLGGLIETIPLAVLAGLLVHV
                     GVNLVNLHHIRDLATHNEHYIYFATVLGVTCVNLLAGVGIGVALSVFFAIRRLSLTEV
                     KMEQRGDKWHVVIEGTMTFACVPKVNLELSKIPAGAPVDIDLAVDFIDHAAFEALHGW
                     RQNHERTGGHVDIDETHEEWYKPAVEGRPRRSKSLRKSTRTAT"
     gene            18511..20631
                     /locus_tag="PLP58_10045"
     CDS             18511..20631
                     /locus_tag="PLP58_10045"
                     /inference="COORDINATES: protein motif:HMM:NF021578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na-translocating system protein MpsB"
                     /protein_id="HRJ08901.1"
                     /translation="MAGPHISPPSTAGPPLSESDTRLRETVLHAAHLLPAQGPIGVFI
                     HHNTLHAFEHLPFDDAVREGSRVFGCEPYLSEDRYRDELARGRILFAELGTVLRRDLG
                     EKAEEPLHGLSSRLEIRLAMLEHPLRSGGGHELDWFMAETDALLKARRDVSEVQRRKL
                     ITETRHWVMRRLRGSLPDVERPAWLDSLFTRFKETRIEEWSDDQWEAFTMSALWEVCG
                     EGVPAATPMIRHRDFIHALGGADTDLMVNDVLIRFCAAFLDQGIAHLPLPGRDEGFYR
                     AFCMLHARGGAASAWWLGGLRAEVERLQKENISALASIRESLDDLGVKPDETEKFLSA
                     TLLALRGWGGMIWHVEQRADRVHHAVPDGSLIDFVAARLLLERLALRAAARSSLGHEG
                     ALSSLREELALRLPSTRPACDKQRAFLIFQLAQVLGWTPEVLFHLEPMGWTVLFDEVE
                     GFDELARRRVFHLAYEHRFRVQTLDALASNTGRAARLPERPSFQAVFCIDEREESIRR
                     HVEELAPTAETFGAAGFFGVVMYYRGAAAADFVPLCPVVVRPQHWVSEKVDRRLSDEA
                     RRRSSTRRRIGMALQSFHGGSRRIVSGAFFSAALGLLATVPLVARVVFPRLTARFRGF
                     FGSFIAPPPVTRLVLERGTPQPGPGEESHGFLPEEMFNITERLLRDVGLTRSFARVIL
                     VFGHGSTSMNNPHESAHDCGAFPR"
BASE COUNT         3777 a         6486 c         6741 g         3668 t
ORIGIN      
        1 ggccgggtca aagccggcgc cgttgtcgct gatctcgacg cgcaggccct cgcgggtggg
       61 cgtgaggtga aagcgcgcgc gggtggcccg ggcgtggcgg gcggtgttgt gcagcacctc
      121 cttgcagaag aggtaaatct cccgccgcgt ctccggatcg ggatcatgcg tcaaaggcgc
      181 ggcgggcagc gcgcagtcca gcgccaggcc gcgcaggagg cgctcggtga ggcggtgcag
      241 cacatccagc cagtcgtgct cgtgctccac gcggcgggtg cgcagcaggt ccaccatgtc
      301 gcgcatggag tcggccgtct cggcggccac gcggccgatc tcgccgaggt cctcgcgcag
      361 cgcgccggcg ctggtcccgg gctgcgcggc catggagcag atgagggtga tgctgccgag
      421 gttggagccg atctcgtcat gcaagtcccg cgccagcttg tcctgcaggc gctgcgcgtc
      481 gagacggcgc tgcctgcgct gccgccagaa catgaggcca agcgccagcg ccgccgcgcc
      541 catgccgctc acgctgccat agacgagctg ctgctgcgcg cgccgggcca gtgtctcgcg
      601 ctcctccacc agccgcgcgc gctccgcctc cagcgcgcgc ctttcctcca aatgcgcgaa
      661 ccactccggc agctcgatga gcctgccgtc ctcggtcagc ccgtcattca gcgcggagag
      721 cgaccactct tttccctccg cctcccgcgc ctcgtcactg gcggtgaagg tgccgcgcgg
      781 ggccaggttt tccccgccgc gcagcgcctg catttccgcg agggcgaaga cgtaatcact
      841 cctgcgatac caaagctggc gcgccgtgac gcggatgaac ttcgccctcg ttccgcgcgc
      901 cggtatgcac accaggttgg ctcccggcgc ggggtggagg cggtcggtga cctcatgcag
      961 cagcaccgcg tcgctgaaat ccgcgcgcgt ggcggcctcc accttgtaga gcgccgggaa
     1021 gccgtagtcg aaccacagcg gcacctcccg cacccgcgcc ggcacgaggc gtatctcatc
     1081 cagcgccgtc atctccggca gctccagcgt gagccatttt tcctcatccg cttttgccgc
     1141 cacggcgctg tggaagccgt gcgttccgcc cgcctgcggc gcgaccggca ggcccagcgg
     1201 tgtgaccatg tcggtgagat tggaccgcgt ccaggcgcgc ggtgagttgc ggctgctgga
     1261 gctgcccacc tgcgcgtcga gcgccgcatt tcgtgtgccg gagagcacca gcatctcggc
     1321 cagcgccagc acctccgggc cgtccacggt ccaggggtcg gtggccgtga ggcgcacccg
     1381 tttcaccggc cttggctcga agcgcgccac cacggggtag cagcccggat ttgggaaatc
     1441 ctccgccgtg cggtccagga tggtgaaggg cgcgtcccgc tcgtcgaaga cctccagctt
     1501 gaagcggatg gggaagccat agcccgccac cacggcggac gcgcccttcg ccagcggcgg
     1561 cacgagcacc acggcatccg ccagggcagg ttcggggagt gtcagctcga tccagcgcac
     1621 atcctccgcc gtggtgtagc ccgtcttcag gccgacgcgg atgcagctgt tcaccagcgc
     1681 catttccgcc agggtggaaa gctccgcgtc caccgcggaa acgcgcgcgt ccagcctgcc
     1741 cagctccgcc cgcgaccaaa aagccagccg ctcgctccag gcgggcgggc cggcctgcgc
     1801 gagcgtggcc gcggccagcg tgaagacgag gcatctcatc atgcgtggcg ccaagcatag
     1861 cgtcatgcat ggaaaagtcg catcccctgt tttggggaga tgccgcgcgg gcgtcagcat
     1921 gagtttgcgc aaagcgtcat ggctgcttca ttcgcgcgtc atgccgcccg ccgcacccca
     1981 cgccccctgg accctcgaag gccgcgcgcc ttacggggag gcggacacgc gggcgaagct
     2041 gattgacccg ctgctttacg cggcgggctg gacggaggag cacatccggc gggaggagac
     2101 ggcgggcggc atcgaggtca tcgagggcga ggcgcggcgg ctctccaagg ggcgggtgga
     2161 ttacacgctg cgcttcaagc tcagcgccag cgtgcagccg gtggcgctgg cctacatcga
     2221 ggccaaggcg gagaccaaag acccggcgga ggggctccag cagggcattg actactcggg
     2281 cgcgtcaaag cgcttcaatg tgccctttgt cttcgccagc aacggccatg ccttcatcga
     2341 gtatgaccac ttcaccggcc aggtctcgcc ggtgcggccc atgagcgact ttcccacgcc
     2401 ggaggccttg cggcagcgct atgagatcgg caaaggcttc tcgctcgact ccgaggccgc
     2461 gcggccgctg ctcatcccct atgcgggggg cgaggcggtg cggcgctatt atcaggatgc
     2521 ggccatccgg gcggtgctgg aggcgctcgc ccgcgggctt ctgcatgtgc tgctctctct
     2581 cgccacgggc gcggggaaga ccttcatcgc ggtgaacctg ctcaagcgca tccgggatgc
     2641 cggcctgctg cgccgggcgc tctttgtgtg cgaccgcgat gagctgcgct cccaggcgct
     2701 cggggccttc cggaatcttt tcggctccga tgtggcggag gtgtatgagg gggctgatgg
     2761 caaaaacaac gcccgcaacg ccgtgatcca catcgccacc taccagacgc tggcagggga
     2821 ccagggcagg gaggcgcgga actttttcgc caggcactac ggcgccaaaa accacttctc
     2881 ccacatcatc attgacgagt gccaccgctc cgcctggggc gactggaagt tcatcctgga
     2941 agacaacccc gatgccgttc acatcggcct taccgccacg ccgaggcagc ttattctcgg
     3001 caaaaaagcc tccgccgcca gccgtgacga ggcgcagtgg cgtgacaacg tcgagtattt
     3061 tggcgagccg gtctatgaat acaccctctc ccagggcatc gaggacggct atctggcccc
     3121 gccggagatt ttcacctttg atcttttcca cgaaggccag accgaggctg agcggctgcg
     3181 gaatgtcacc cgcgccgagc tggaggcgaa aaagctcaaa agcagcgcca ccggcgcgcc
     3241 catcacctcc gcgaatctga aggaggaata cagcccgagc gagctggatg agcgcctgct
     3301 gctgccggag cgggtgctgg ctctctccca gcatctgctg gagcagctca tcgcccacgg
     3361 cggcacgccg gagcagaaga ccatcctctt ctgcgccagt gaccgccatg cggaggccat
     3421 gagcgagcag ctcggcaatc tctaccgcga ctggtgcctg gcgcacgggc aggagccaaa
     3481 ggcgtatttt gccttcaaat gcaccgccaa gtccggcggc acggatcatg tgcccgatct
     3541 gcgcggcagc gcctcctcgc acttcatcgc ctgcacggtg gatctgctca gcaccggcgt
     3601 ggacgtgccc tgcgtgcaaa acatcgcctt catgcagtat gtgcgcagtc ccatcgtctt
     3661 cgcgcaaatg ctcgggcgcg gcacaaggct ggacccggac agcggaaaga tgatgttccg
     3721 cgtgtttgac tacacggagg ccaccgcgct gctggggcgg gactttttga cgaagttcaa
     3781 aggcggcgcg tcccgccaga aacagaaacc gccgcccgcc gagccgcccg ccgtggtgga
     3841 gggagtaatg atccgcatcg agcccaccgg gcgctacctc accgccacgg tggagggccg
     3901 ccacgcccgc gtcacgctgg agcagtaccg cgagcacatc gcgcagcgcc tcatcgcgca
     3961 ggcgggcagc atcgccgact tccgcaggct gtgggtgctg ccgccggagc gccgcagcct
     4021 catcgacagc atcgtgcgcg gcggtttctc accccgcgcc ctgcaagtgg ccgaggaagc
     4081 cgccgaatgc gatctctacg acgtgctggg agaggtgggc tacggcatcg tccgccagac
     4141 gcgggcggac cgcgcctatg ccttcaccta caagcagcag ccctggctcc agctcatgcc
     4201 cccgcccacc gccgccaccg tgaaggcgat agcaaaccag ttctccctcg gcggcaccga
     4261 ggcgctggag acgccgcaca tctttgacgc gcccatggtc gtcaaagccg gcggcattcc
     4321 cgcgctgcgg ctgtttgatg aaccggcgaa actgctcacg ggaacgaaag agaggttgtt
     4381 tgcggcatga gtggaatacc aaccgtcaaa cttcgtgact gcacgaccaa aataggcagt
     4441 ggaacgactc cgagaggtgg gcatactgca tacaagcagt ctggaatccc gctcatccgc
     4501 agcatgaatg tcaggatgaa tggtttttct cgcgacgggc tcgcattcat tgatgaggag
     4561 caagactctt cgatgtcagg aactcgtgtg cagcctgctg atgtattgct caacatcaca
     4621 ggtgcatcga ttggtcgcgt ttgcatcgca cctgatgaaa tctgtccagc aaacgtcaat
     4681 caacacgtct gcattattcg aagtaatggc aggctggaac ctgaatggat cgctttcttc
     4741 atctcacagc ctgatttcca gagtcagatt ctgggacaac aggcaggggc cacgagacaa
     4801 gctcttacca aggagatgat cgagcagttt gagcttccag cgcccgatct ccccacccaa
     4861 cgccgcctcg ccacgcgcct gaaatcccaa atggccaccg tcgaagaaac ccgccgacgg
     4921 gtcgaggagc agttcaagca ttgcgaaacg ttgaggctca agttctcagc cgaagcattc
     4981 gccccagaag tcctatttca gtgggaggaa cgcccgctgc tcgaaatgag caagatcgtc
     5041 tcgggcgtga ccttgggcgg tccgacggcg aaggaaccaa caagaaaagt gccctatctt
     5101 cgcgtcgcga acgtgaagga tggttggttg atgcttgatg atgtgaagga gatagacgct
     5161 actgagagag aaattggaca gttcagtctg aagcatggcg acgtcgtctt gaccgaaggc
     5221 ggtgaccccg acaagctagg acgcggaacc gtttggcggg aagaaatcgg tctgtgcctg
     5281 caccagaatc acatctttcg agtgcgtttc gatcaagatc aagtttgtcc cgagttcgct
     5341 gcgtatcagt tcgcatcgaa atacggcaaa gcctactttc tggcgcacgc caagcagacg
     5401 acaggcatcg cttcgatcaa caaaggtgtt ctttcacgtt tcccatttcg cctcccgccg
     5461 ctgaaaaccc agcgccagat cgccgcgcgg ctgcgggagc agttcacgca cctccggcag
     5521 ctccaaaacg ccctgaggac ccagctcgaa gccctcgaca agctgccggg cgcgttcttg
     5581 cgggaggcgt ttgggagttt ggcggggtaa atcacgttct ttcgccactc atgacctttg
     5641 tgccaccgca tcatcattgg aagcccaccc gcattcgcgg tctcgtgcag gctttgccct
     5701 cgggatcaga ggcgctgctg gtggagacgg acgctggcgc ggcctatgcc aagctgcccg
     5761 gtggcaagga aggcccacac atcctcgcct gcgagctggt gggcacccgg ctggcggcac
     5821 ttttggggct gccggtgctg gattgggtgc tgatgcctta tccgggccaa ccggccctga
     5881 aactgagatc cggcaggatg gcccaggcgg gaacggcctg gctgacccgg aaggtgaatg
     5941 gctttggctg gggtcgggaa gcctcagacc tgaaacacat ctccaatcgg gccgacatcg
     6001 ccaaactggt gattctggac cagtggacgc ggaactgtga ccgctaccga ccggagccga
     6061 aactgcgcgt gaacgagaac aacgtcttcc tcaaccggga aggtgctccg atcggccagt
     6121 tcatcctcat ggcgatggat cacacgcaca ttttcacctg tggcgggccg ctgatgcggg
     6181 acatggccaa aattgatctc gtgaaggatg tgacggcgtt tggcctgttt cccgagtttg
     6241 agggcgccat ccagcgtgcg gacgccgtgg cggcggcagc ggctctggcg gcggtcacgg
     6301 acgaggcgat ccatgagatc gtggcctcca tcccggcaga ttgggaggtg gacggcacga
     6361 tccgggctgc gatggaagat ttcctgcgcc agcgccgtga ctggctgtcg aaaacttttg
     6421 tgtcccgact ttttccccag gacgaacttg cgctagtata gaccactcca accaccatga
     6481 acacacccac caccggctac tacagcctga tccaatactg cccggaccgg atgcgtctgg
     6541 aggcagcaaa catcggggtg ctgctgtttt gccccgagcg gaaattcctc caagccaggc
     6601 tggcatcgtc caaccggcgc attgaccgct tcttcggagc ggaggcgggg gatttgaagc
     6661 agatcaatgc catgaagaag attctggagc accgtctggc tgccgaatcg cagaaaattc
     6721 aggatgtgga gggacttcag cactttgccg aactgctggc gaacgagatc gtgatcaccg
     6781 agcctcgtgc gtggctggtg gaggaacctg aggtggagct ggcacacctg tacgagcaac
     6841 tggtgggccg gttcatcaag tcggaaccag gacctgaaaa gctgctgctg gagaagctgc
     6901 gactccagct tgaaacgccc cgcatccaag ccgtgctcaa gcgtgacgtc gtggtgaagg
     6961 tgccggtgtt cggaaacgaa ctgaaagtgc cctacgccta ccagaatggc cgcctgaacc
     7021 tgatcgaggc gcatgagttc aaccaacagc gggagcagga catcatcagc gacgtgctga
     7081 cgaaggccgc ccagggcaac ctcatttaca aacatcccga cccggaagct ggcgaacgtc
     7141 agcttgtggt cgtggcctcc tttggtcgtg ccgccgtaga gcaccgccag caggtggaaa
     7201 ccgtcctgcg ggatcatcat gtgacctttg tgccggacac cgggctggct gacttggagc
     7261 ggcagatcgc ggagacggcg cattgaggca ggttttagcc cctgttagtc gtcgaactac
     7321 atttagtccg acgactaaat gtagcctcca ggctgaacac cacttaatcc atgcccaaac
     7381 ccgccaaacc gaagcccaaa acgaccaccc gctccgggct ggacagcgcg atctggggcg
     7441 tgtgtgacat tctgcggcgg tcgaactgcg cgggggcgct gcagtatgtg ccggagctga
     7501 cgtggatcct tttcctccgc atcctggacg agacggagga gatcgaggcg atgaaggcgg
     7561 cggtggtggg cgcggagttc cggccctcgc tggaggcgcc gttccgctgg cgggactggg
     7621 ccgcgccgga ggccccggag cgcaagaagc tggtggagag cacgacgaac gccttttacg
     7681 acttcgtgaa cacgcggctc atcccgcacc tgaaggcgct gcgggacaaa ccgaacgcca
     7741 cgccgcgcca gaaggtgatg agcgagatca tgtccagcgt ggaccgcacg cgggtggaca
     7801 cccagcggaa cctgctcgat gtgctggaca agattcacga gatcagcctc gacacggtgg
     7861 acgacacgca cgtcttcgcc ctgagtcaga tttacgaggg cctgctgctc aagatgggcg
     7921 agaagggcaa tgacggcggg cagttcttta ccccgcgcga ggtcatccgc gccatcgtgg
     7981 aggtgatcgc gcccaaagtg ggggaaaccg tgtgcgatcc ctgctgcggc acgggcggct
     8041 ttttggcgca ggcgtatgag ttcatgcgcg gacaactcgg cgaaaacgcc accggcgacc
     8101 agatcgagac gctgaagcac aagaccttct acggccgcga gaaagagaac ctggtctatc
     8161 ccatcgccct cgcgaacctc gtgctgcacg gcattgatga accgcacctc tggcatggca
     8221 acacgctgac ccgcgaggaa atctacggcg gtctctatca gaacgcgccg gggcagtttg
     8281 aggtgattgt gaccaatccg ccctttggcg gcaaggaggg cgaggacgcg caaacgcgct
     8341 tcgcctacaa atcccgcgcc acggaggtgc ttttcctcca ggatgtgatg gagaccctga
     8401 aaactggtgg gcgctgcgcc atcgtgctgc cggaaggcat cctgtttcag accaaccagg
     8461 acgcctttgt gaaaaccaag cgccgcctgc tcgacacctg cgacctttgg tgcgtgctca
     8521 gcctgcccgg cggcgtcttc actgccaccg gagccggtgt gaagacgaac gtgctttttt
     8581 tcaccaaggg caggcccacg gagaagattt ggtatttcga cctcagcgat gtgaaggtgg
     8641 cgaagaagac gcccttcacg cgccagcaac tcgcggagtt cttcaagctc ctgcctgacc
     8701 gcgctgacag cgaacgaagc tggaccctcg acatcgcgaa acgccgccgt gacgccaaga
     8761 cagaggccga caagctccgc gccaccaccg ccgagcccaa agcccggctc aagaagcatc
     8821 aagagcagct cgatctgctc aagaaagcca aagccaagcc cgggaagctg gaggaggtca
     8881 aaaagctcat caccgcctgc gaacgcgaaa tccgcgagat cgagtccaag gcccagacca
     8941 tcgaggacgc ggcctttgac ctcaaagccg tcaatcccaa cgccaagagc accgaggaca
     9001 cccgcagcag cgctgagctg atggatttga tcgagacgaa gggcatggaa gtgctggagt
     9061 tgctgggcac tctgcggagt ctttgaggca ttttattcgc atcccctgtt ttggggagat
     9121 gtgaaggcgg ggggaagatg cgttgatggg agtgatgaaa gtcacctctc tttttgcatt
     9181 cctcattctc gccgtggcgg cgcatggcca gacggctgac gcgcctgccg agaaaaaagc
     9241 gccggtctcc agcaccaagg tcatcccgcc ggacatcaat ggttacaaaa ccatcatcgc
     9301 cccgccggag aatcccgcgc cgttgaagat cgccatcttc gagggcaagg gcgcgccggg
     9361 cggcggcatt gagaatgtct gcgcgaaggt ggagctgatg cccggctcga cgatcacgcg
     9421 gctgaagccg gaggagatcg ccagtggcgg gttgaaaggc tatgacgtcg tcgttttctc
     9481 cggcggcagc ggcagcgcgc aggcggcgag cctgggggat gagggcaggg aaaaggtgcg
     9541 cgagtttgtg aaggacggcg gcggctatgt gggcatctgc gcgggggctt acctggcgtg
     9601 ctcgaatttt tcctggggtc tcggcattct gaacgcctcc accgtctcca gcaaatggat
     9661 gcgcggcagc ggctacatgg acgcggaggt gacggtggat ggcgctccga tcctgggacc
     9721 ggtggagggc gtctttaaag tgcgctacaa caacggcccc atcatcaagc ccggcacccg
     9781 cgccgacctg cccgcctacc gcccgctggc gctcttccgc tccgaggtgg cgaacaatgg
     9841 cacgccggtg ggcgtgatgg tgaactcccc cgcgcaggcg gtttccactt ttggcaaagg
     9901 ccgcgtcttc atctccagcc cgcaccctga gaacaccccc ggcctggagc acctcatccc
     9961 gcgcggcatc tactgggccg ccgggaagct ggcggagaaa cccctcgtcc acgtgccatg
    10021 agggcgggca tccacatcgc cgccctgctg tgcgcgctcg tggcggcgcg cgcggatgac
    10081 gaccgggcgg cttgcaagac accctgcacc gtcgagttca gcttcaagcc cgggggagct
    10141 ggtgcttcac ccagggcagc catgtgttga ggatcatccc ggacggcatg tgaaaacgcg
    10201 tgcgcgcctc tgtggcgcgg ggcagtgacc cgcgaggggc gcgcattccg gcgtcactgc
    10261 cgccggctac agggggcccc gtaggcgcgc ttgccaaagc gcgatctgcg tccccgctcg
    10321 cacgcataat ccccgcactc tggcgaacgc gcctgcgggg cggccgcgct tgaggatcac
    10381 aggatgcgga tctgggcggg gtcactccac acgcccttga tgtttttgat gcccagggtg
    10441 cgcacgcgcg cccagaccac cgcgcccggc gggaagcccg tgatctccgc gcgcccgcct
    10501 ttgatgatgc ctttctgcac ccagtccgcc tcgttgttcg gatcacccgc gcaggtctgc
    10561 acctcgttgg tgctgttggg cttccgcggt ttgtagcggg cgatgaagcc gcccggcagc
    10621 ccgctgtgcc tcaggcgcag gttcgcgggc gcgccgggcg gcgtggtgtc cggcgcgcgg
    10681 ttcacctcat aaaaagggaa gccgcttttc tccacgatcc cgccatctcc cttcgccacc
    10741 acgttcacat aattgccgag cgagttcagc gtctcctcca gcaagtcacg cgccgccttc
    10801 aaggccatca catccgccct ggccaggctg gcgcgggcca ccagcctggc tttataatcg
    10861 gtgatccgct cctggaacgt ggccatgtcc acgggcggag cgtcaaagat gccggcgttg
    10921 agggtcatct tgtcgtggat gacctgcgcc gccgggctga gtttggcggc ggtgtgattt
    10981 ttgaaactga cggcggcttt gatggtgctc atggcgatgc ggggtggcgc cggcggcgtg
    11041 tggcagggca acgcggcggg catggttcag catgggtttg gaggtttccg ggtctcgccg
    11101 cctccccggc ggcaaaaaca cgcgccccgg cggacagccg cacggcggac ccgcgcggac
    11161 acaagaggga gcgctggaaa aacatgctgc cccgcatccc tcccaggctc agaaggccgg
    11221 aacccttgct ggatcgcacc gttccccatt ggaaatgccg cgttccccat tggaaatgcg
    11281 ccatccccca ttgggaatgt ggcgttcccc attggaaaaa ccgcatcttg accatcgagc
    11341 gcgttcttcc caaacgcgaa tcccatgtcc tcaccgcaga gtcatgcggc gggtggatgg
    11401 ggcggcggat cggattcatc gcggtgaaaa aatcatttca cgcgcacttc agcacgacat
    11461 gcggatgccg cgttagccaa aagaaattgg gtaatggcgc agccccgcgc tttcacgcct
    11521 ttgcctcagc ggcaagctca gcgaaccggc ccacgggacg ccacgattgc aaccacgacg
    11581 ctcacgccgg gttcgctgca gcgcatggtt agactgccaa atatcaacgt gtccgaacat
    11641 tctctctccg aagtttcagg tatcgctcaa cgtcgccacg ctcgaccgct gcgacggcct
    11701 ctgcagccgt gcagtcgctg gcgatacaac cgccctgagt ctgaacccgt gaaccctctt
    11761 tggcaaatct cgcggcacag tctctaaaac tggcgctgtc agtgaggtga aggccagcaa
    11821 gcgcgtagag tctcgcctcg gtcgtagtgc tctgctcgaa taggtcgtaa aacaatcgcg
    11881 ccgagtgccg gctggaaaga atggcaaaga accaatcctc gcgctccggt atcgaacccg
    11941 cgacgccaac tccaccgaag gaaaatctat ctgccttcgc gagctgagca acagcaatcc
    12001 ggccaacctc tgagggtgga agcaactgat acgcgatgac gccgatgaca accgctaaca
    12061 cggcaacaat caccagacgt ttcaggagca cgcgcatggc aggctaacgt tcaaactgag
    12121 gcgaccgctg gggcgggggt ggacgtcgaa tcggcggtgg atgttgaagt tgaagaaaag
    12181 catctcgggc acagagcccc ggcggttgcc tccagtgctt tgttcgcctt catttggtgg
    12241 tgattgctcg tcatacttgt ttgcgtcgat cgtctcgcgg tcgtggctgt gcctgaatcg
    12301 caaggctggt aaatccaaaa gccatccgat gctcaacgtc aaccaatgag agctgctggc
    12361 aaagtcgcat gactagcccg ggaatacgat atggcgaatg aaagtcaaaa agacccggac
    12421 tccaagatgc gttcgaaata cacttcgctc cgacctcggc gatggaaaaa tgggtgccct
    12481 cagcggatgt gtgctctaca gcagccaaag actctcgcct gatactgtcg aataagtctc
    12541 tgccttgatg ccaatcaatc tcacgagtaa ggaggtcatc ccatgtgtcc cagtatccgc
    12601 gatgtgggag gtgtggcctg aactctcgat gcaaggcaca gacccaatga acgcaccacc
    12661 gccaatcgcg acacgagcaa atggcctgcc caagctcaag taagtccttc gaggacttgc
    12721 ctgtcagcgg atgctccttc tgtggcatgg taaatggcgc tcagttgttg ggcgaacgtc
    12781 aaagagcacg cacccctatc agcgggggtg agcgtcgatc acggggttga ggttaaaact
    12841 aggaaagagc atgaaaacag ggcggctgat aggggttgcg tgtctcgact tgttcggttt
    12901 ctgttctagt catggaggtc tcccgctaga atccgtgcca aaaatccagc aaaactgtct
    12961 gctcgaaact ctgtctcgcc agtctcatgt gaccacagaa cgactgttgg aataggactc
    13021 gaccccgagt agtcgaagca gtagtagtcc ccggacccga agtcagcgaa tggaaggagg
    13081 tgctcgaacg agcgatcttc ctcatcgaag ttcgcgagcc actgctgcca tccgttcttg
    13141 aaattgtgca cgatggactc ccatgtcttc cgagggtctc gttcgtcgcg gacgggatag
    13201 atgtggacgt aatcgattcc tgtcccgttg tgctcgatca gccactcacg gaaatctggt
    13261 ggcaacctac gaccaaggtc agcttctgct gcggctattg attcctctgt ggtgccgatt
    13321 gtgcgaggcc tcttcatatc ttaccgaacg ttgtttagcc acacgaatgt atcgtcgaac
    13381 gggactcgac gagagtttgg tgtgaatgag gggggagatt cgtccgcgac atggccggag
    13441 gtgaggaatc ttttttcatg atgggaacga gccaagcgaa aaccgtgccc ggcgtcaaga
    13501 ccttggagaa gaaaatccca gcggttccgc cgcgccaggg atgttgcggc gcgccgcgtg
    13561 tttgacgcgc attccggggg cactagacgc tgatgacaag gcaccccgca ggcgcgcttg
    13621 ccatgcaggc agagcggctt tccgtttttc ccgccatgtc aggcggtcaa actgcggcgg
    13681 cgatgatcaa tggcaggggc tgcacgctgg attcgggggt ctggaagaaa tccaccttgc
    13741 ccgtgccgac atcgtaaagg gcgccgacga tggcgatttt gccctcgttg acgagcttgt
    13801 cgagcgtggc gctgcgctgg cggatgacct tgatggtgcg gaccacgttg aggcgggcca
    13861 tttcgttggc gaaggcggcc ttttcggcgg gagaggccca ctcgccggct ttcttgagcc
    13921 tgctggtgtc cacggcctgc tggatctcgg tgacgaggcc gtcgaggttg ccgcaggggg
    13981 tggcctcgct ggcttttttg gcggcggcga gcaggtccac ggcggcgttg acggcgccgc
    14041 aggaggtgtg gccgaggacg acgacgagtt tggagccgac gacggcgcag gcgtattcga
    14101 ggctgccgag cagctcgggg gtggcgatgt tgccggcgac gcgggcgctg aagacatcgc
    14161 ccagcccgag gtcgaagacg acctccgccg gagcgcggga gtcaatgcag ccgagcacgg
    14221 cggccatggg gaactggccg gcggaggtgg cgttgacgac gcggctgtgg tcgcggatga
    14281 gggtttgttt gttgaggaag cgctcgttgc cttccttgag tatctgcagc acgcggtctg
    14341 gcgtgagggt gctctgcact tcgcgggttg agtagtccac gaactggatg cggtcctcga
    14401 gatggtagcg gtctttgaag ccgacgaggc tcacctggac gccgtgcgcc gaggcggtgt
    14461 ccttgcggaa gtcggcgatg aggtcgagca cgtcggggtc aatgtagtcg gtgtcgcgcg
    14521 cgtcgaggag gacgtggccg ccgcgcggca cctcgaagag ggttttctcc agcgcggggc
    14581 ggctgaggaa gctgacctgg ttggccagct cgatgcgcag cacctcgccg ccgacatggt
    14641 tttcacggat gcggcgcagc ggccggcgca ggttgctgtg caggaggaag agaacgctga
    14701 cgccgaggcc gatgaggatg ccgcgcaggg ggtcggtcag cacgatggcg atgacggtga
    14761 tgatgaaggg ggcgaactgg ttgcggccct cgctccacat ctgccgcaga agcgcggggg
    14821 aggccaggcg caggccggtc atgaacagca gcgcggcgac ggcggcgagc ggtatttcat
    14881 tgagcaggcc gggcaggaac atgacgcaca gcagcagcag gaatccctgc gtgatggcgc
    14941 tgagcttggt gcgcgcgccg gcgttgatgt tcacggagct gcgcacgatg acgctggtca
    15001 ggggaaggcc gcccaggagt ccgcaggcga tgttgcccgc gccctgggcg acgagctcgc
    15061 ggttcggcgg ggcgcggcgc tggtcggggt caattttgtc caccgcgtcg agattgagca
    15121 gtgtctccag actggcgacc gcggcgatga gcagcgcggt ggtgtagatg gcgggattgg
    15181 aaagctggga aaaatccggc gagcggaaca tgctgaaaaa atccccgatg ctgccggcga
    15241 ccgggacctg caccaggtgg gagggctcga tgaccaggct gccgccgatg gagcggaaga
    15301 gcgcgttggc aaggaccgcc agcacgatca cgaccaccgc cgagggcacg gggaactttt
    15361 tgagcgaggc gacacggtcc cacacaagga cgaggacgat ggaaaacagg ccgacgagcg
    15421 ccgcgcctgg atgaatgtca atcaaggaga ggaacagctc ggagaaggtg ttctcattgt
    15481 ccggctggaa gaaggacttg tcgcccatcg ggtcggcgtc gtggccgaga aggtgcggga
    15541 tttgcttcaa aatcaaaatc acgccgatgc cggcgagcag gcccttcacc acgctgcccg
    15601 ggaagaagac cgccacaaag ccggcgcgca ggacgccgag cagcacctgg atgacaccgg
    15661 ccacgagcac cgcgagcagg aatgactcga aactgccgag gacagccatg ctggagatgg
    15721 tgatggagat gaggccggcc gccgggccgc tgacactttt gtgcgagccg ctcagagcgc
    15781 cgacgacgat gccgccgatg atgcccgcca ggattcccga gatgggcggt gatccagacg
    15841 cgacagccac gcccatgcac aggggcaggg cgacgagaaa gaggacgacg ctcgcggcga
    15901 ggtccttggg cagggtggca tggagcgggg ctttgttgtg gggcatggcg tgaatcaacc
    15961 tggaggttca atgcggcttg agcaacaaaa gagccgccgt ggacgtttga aggaatcaat
    16021 gaatcagatc aacacttaga tttgctcaaa tctatcatgc aatccgccga gctgaactac
    16081 caccacctgc gccttttctg ggaggccgcc cgcagcggcg ggctcaggga ggcggcggag
    16141 aggctcgacc tgtcccagcc caccctcagc gcgcagatca agactctgga ggagtcgctt
    16201 ggcgaaaagc tcttcgagcg ttccgggcgc ggcttgaagc tcacggccaa ggggcgccag
    16261 gtcatggatc atgcggggcg gatattcgcg cttggcggcg agatgatccg ctccttgtcc
    16321 gagggcggct cggccatgtc accttgcttg aatctgggcg tcacccagtc gctgccgaaa
    16381 ctggtgtcct ggcgtctgat cagcaaggcg gtccatgaaa tgcccggcct gcatttcacc
    16441 tgccatgagg gccaggcggc ggacctcgtc ggccagttgg tctccggcag gctggacgtg
    16501 gtgctggcgg acgaatccgc gcccgcctcg ctgcgggtgc gggcttttga ccgcgtggtt
    16561 gacaggtccc gggtggtctt ctgcgccgcg aagccgctgg ccaggcggtg gaaggcggat
    16621 tttccgaagt cgctgaatca ggccccggtc gtgctgccct ccatgcacag cgcatggagg
    16681 cgggagctgg accgctggtt cgagtcgaaa aaaatcacgc cccgcatcgc ggctgagttt
    16741 gacgactcgg ctttgatgaa gaccgcggcc gcggacggcc tgggcattgt tcccatcgcc
    16801 tcgtccgtgc ttgaggaggc ggtggaccga tatgacctga cgcctttcgg gccgccggtt
    16861 cagtgctgct ttgaaagcca tctcatcacc cttgagaaga ccctgcgtca tccagcgctc
    16921 gcagccctaa ccgccgctgt tgccagtggc ggtaaggtca tgcctgtctg attgactcgc
    16981 cgtcaatcca accttaaccc cagctcaaac ccatgaacct ccgccgcgat cttcccgcct
    17041 cccttgttgt cttcctcgtt gccgtgccgc tttcgctcgg catcgccctc gcctccggcg
    17101 cgcccatcat cgccgggctc atcggcgcgg tggcgggcgg cattgtcacc ggtctgctgg
    17161 cggggtcgcc attgcaggtc tcaggtccgg cggccggcct gactgtggtg gtggccgggc
    17221 tggtgcatca gtttggctgg gagacgatgt gcctgatcac cgcctgcgcg ggcgtggtgc
    17281 agctcatcct cggctttctg aaagtggcgc gcggcacgct ggtcatcgct cccgccgtgg
    17341 tccacggaat gctcgccggc atcggcatct ccatcgcgct ggcgcagatt catgtggtct
    17401 tcggcggatc gccggccagc tcgcccatcg cgaacgtgct ggcattcccg aggcaactgg
    17461 ccgccatgag cggtgaggcc gccattctcg gcttcctcac catcgccatt ttgatcgtgt
    17521 ggaaaaagct gcctgtgccc gcgctgaagg ccgtgcccgc gcccctggtc gcggtcctgg
    17581 tggcgacggt ggtgtccgtc attttcaaga tggatgtggc gcgcgtggac ctgccggaga
    17641 aattcgagct cacgcagttg aagccctctt cggactgggg cgccttcatc atcgccgtgc
    17701 tcaccgtggc gatcatcgcc agcatcgagt ccctcctttg cgcggtggcc accgacaagc
    17761 tgcactccgg cgagcgctcg aaactggaca aggagctctc cgcccagggc gcgggcaatc
    17821 tcgtctccgg cctgctcggc ggcctgccga tcacgggcgt cattgtgcgc tcctcggcca
    17881 acatcatggc gggggcggct tcgcgctggt cggccatctt gcatggcgtg tggattctgc
    17941 tgttcgccct gttcctgggc ggcctcatcg agaccatccc gctcgccgtg ctggcaggcc
    18001 tgctcgtgca tgtgggcgtg aacctggtga acctgcacca catccgcgac ctcgcgacgc
    18061 acaacgagca ctacatctac tttgccaccg tgctcggcgt cacctgtgtg aacctgctgg
    18121 ccggtgtcgg catcggcgtg gcgctttccg tgttcttcgc catccgccgc ctgtccctca
    18181 ccgaggtgaa gatggagcag cgcggtgaca aatggcatgt cgtcatcgag ggcaccatga
    18241 cctttgcctg cgtgcccaag gtaaacctcg aactttcaaa aatccccgcc ggcgcgccgg
    18301 tggacattga ccttgcggtg gacttcattg accacgccgc cttcgaggcc ctgcacggct
    18361 ggcgtcagaa ccacgagcgc accggcggcc atgtggacat tgatgaaacc cacgaggagt
    18421 ggtacaaacc cgcggtcgaa ggcaggcccc ggcgctccaa atccctgcgc aagagcacca
    18481 ggaccgcgac gtaaatcctg aaaccgaatc atggccggcc cgcacatctc cccaccatcc
    18541 accgccggcc cgcccctttc cgaatcagac acgcggctga gagagaccgt gctgcacgcc
    18601 gcgcatctgc tgccggcgca gggacccatc ggcgtcttca ttcaccacaa cacgcttcac
    18661 gccttcgagc acctgccttt cgacgatgcg gtgcgggagg gatcgcgcgt gttcggctgc
    18721 gagccctacc tctccgagga ccgctaccgc gacgagctgg cgcgcgggcg cattcttttc
    18781 gccgagctgg gcaccgtgct gcggcgtgac ctgggtgaga aggccgagga gccgctgcac
    18841 ggcttgtcct cgcgtctgga aatccgcctc gccatgctgg agcacccgct gcgcagcggc
    18901 ggggggcatg agctggactg gttcatggcg gagaccgacg ccctgctcaa ggcccggcgg
    18961 gatgtctccg aggtgcagcg ccgcaagctc atcaccgaga cgcgccactg ggtcatgcgc
    19021 cgcctgcgcg gctcgctgcc ggatgtcgag cggcccgcct ggctggacag tctcttcacc
    19081 cgtttcaaag agacccgcat cgaggagtgg agtgatgatc aatgggaggc attcaccatg
    19141 tccgcgctct gggaagtctg cggcgagggc gtgccagccg ccacgccgat gatccggcac
    19201 cgcgacttca tccacgcgct cggaggcgcg gacacggacc tcatggtcaa tgacgtgctc
    19261 atccgcttct gcgccgcgtt tctggatcag ggcatcgccc acctgccact gcccggacgc
    19321 gacgagggct tttaccgcgc gttttgcatg ctgcacgcgc ggggaggcgc ggcctcggcc
    19381 tggtggctgg gcggtctgcg cgccgaagtg gagcggctgc aaaaggagaa catcagcgcc
    19441 ctggccagca tccgcgagtc gctcgacgac ctcggcgtga agccggatga aacggagaaa
    19501 ttcctctccg ccaccctgct ggccctgcgc ggctggggcg gcatgatctg gcatgtggag
    19561 cagcgcgccg accgcgtcca tcacgccgtg cccgacggca gcctcattga tttcgtggcg
    19621 gcgcgcctgc tgctggaaag gctggccctg cgcgccgccg cgcggtcatc gctggggcat
    19681 gagggcgcgc tttcatcact gagagaagag cttgcgctcc ggctgccctc cacacgcccg
    19741 gcctgcgaca agcagcgcgc gtttttgatc ttccagctcg cccaggtgct gggctggacg
    19801 ccggaggttc tttttcatct ggagccgatg ggctggacag ttttgttcga cgaggtcgag
    19861 ggattcgacg agctggcgcg gcggcgtgtt tttcacctcg catacgagca ccgctttcgt
    19921 gtgcagacgc tcgacgcgct cgcctcgaac accggccgcg ccgcgcgcct gccggagcgg
    19981 ccgtcattcc aggccgtgtt ctgcattgac gagcgggagg agtccatccg caggcatgtc
    20041 gaggagttgg cgcccacggc ggagaccttt ggcgcggcgg gcttcttcgg cgtggtcatg
    20101 tattaccggg gcgcggcggc cgcggatttc gtgccgcttt gtccggtcgt ggtcaggccg
    20161 cagcactggg tttctgaaaa agtggaccgc cgtttgtcgg acgaggcgcg gcggcgcagc
    20221 agcacgcggc ggcgcatcgg catggccctg cagagtttcc acggcggcag ccgacgcatc
    20281 gtcagcggcg cgtttttttc cgccgcgctc ggcctgctgg ccacggtgcc gctcgtggcg
    20341 cgggtggtgt ttccacggct gaccgcgcgc ttccgcgggt tcttcggcag cttcatcgcg
    20401 ccgccgccgg tgacgcggct cgtgctggag cgcggcacgc cgcagcccgg tcccggcgag
    20461 gaaagccacg ggttcctgcc ggaggagatg ttcaacatca ccgagcgcct gttgcgggac
    20521 gtgggtctga cccggagttt cgcgcgcgtc atcctcgtct tcggccacgg ctccaccagc
    20581 atgaacaatc cgcacgagtc cgcgcatgac tgcggcgcct tcccgcgctg attataccgg
    20641 gcagcggctg cggccgggcc ggaactgcca ct
//
LOCUS       DAOMYJ010000078        20637 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Prosthecobacter sp. isolate AMR_MDS_5629
            NODE_903_length_20637_cov_21.400496, whole genome shotgun sequence.
ACCESSION   DAOMYJ010000078 DAOMYJ010000000
VERSION     DAOMYJ010000078.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867850
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prosthecobacter sp. (wastewater metagenome)
  ORGANISM  Prosthecobacter sp.
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Verrucomicrobiaceae; Prosthecobacter.
REFERENCE   1  (bases 1 to 20637)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 20637)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.30
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:34:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,742
            CDSs (total)                      :: 4,684
            Genes (coding)                    :: 4,676
            CDSs (with protein)               :: 4,676
            Genes (RNA)                       :: 58
            tRNAs                             :: 55
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20637
                     /organism="Prosthecobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_903_length_20637_cov_21.400496"
                     /isolate="AMR_MDS_5629"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1965333"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2775)
                     /locus_tag="PLP58_10050"
     CDS             complement(<1..2775)
                     /locus_tag="PLP58_10050"
                     /inference="COORDINATES: protein motif:HMM:NF012200.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choice-of-anchor D domain-containing protein"
                     /protein_id="HRJ08902.1"
                     /translation="MNPPRFLLAATAFMLALPAYSQSPEISVSGNGQNISNMAGPPSL
                     GDYRDFGSTDMNGGTVTRTFTIHNSGTDLLFGDIMILNYTHTRDTSYFINGPTGFTID
                     PGGMVNFTVTFDPSEPYLNNAEILIINNDDDEANFFFFISGYGEPPDIVVNGNGMEIA
                     DGDTTPSMTDHTDFGNVAVDDGTVTRVFAVNNFDLGTLHLTGSPRVALSGSSAFRVTS
                     QPVLATLGSFGSTEFEITFDPSEPGLATATVSIANDDYYKNPYTFVISGVGDGPKIAV
                     SGKGVNISNNDVIPSESDGTDFGNVAVTNGMEETDGTLTNIFTISNSGMVELKLTGNP
                     LVSVSGDTAFSVSGQPLSSTVAAEGGTVSFAITFDPEIDDTVSALVSIASDDQDKNPF
                     VFAVSGTGTVPEINLIGNGADIANGDVTPSNLDDTDFGKVDVDDDTTIRTFTIQNSGL
                     ATLELTGTPPVALSGSSAFTVTTQPASSSVEAEGGMVSFAVTYDPGSEDTDEAVVSIA
                     SNDDDETPFTFSIKGEGVKPRISLSGNGVNIVNGDVIPGLLDNTDFGGTPAVGGILSR
                     TFTIHNPGEGTLKLTGNPRVAISGSNAFVVFGQPTATVEPDNGSTFFSITFNPALEGI
                     ANAMVSIASNAENSPLFSFAIQGTGTGLPDTKKPTLKLVTPAGKMVSAALPLTVAGVA
                     GDNVAVDRVEVVLNGGAPLLADLGASSKPTAVPFTAQILPVVGDNTLVVTVYDPSGNS
                     TSVTRVFTFERRYLLTLVRAVPAALEAAPDKAGTLSPKAAVAKNATALTKGNPQASQV
                     LPGTLMTLTAAAKTGHIFSHWTGLPEGTQVHGNVVVFAMPEADTNVRAVFVANPFIQG
                     AFAALGPKPLFQGLLRPDEETPSGNDTVGFLSAALVPAKGSLSGKLWMDGLVVAFTGA
                     LHGDGSVWLKS"
     gene            3518..4636
                     /gene="sucB"
                     /locus_tag="PLP58_10055"
     CDS             3518..4636
                     /gene="sucB"
                     /locus_tag="PLP58_10055"
                     /EC_number="2.3.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018290471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045252 - oxoglutarate dehydrogenase
                     complex [Evidence IEA];
                     GO_function: GO:0004149 - dihydrolipoyllysine-residue
                     succinyltransferase activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA];
                     GO_process: GO:0006554 - lysine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyllysine-residue succinyltransferase"
                     /protein_id="HRJ08903.1"
                     /translation="MAEIKIPALGESVTGGQISKWHFKTGDIVKAGDVLMTLETDKVA
                     AEITAETSGELSISMAEGADVEIGTVVGSIAEGKTAPKEDKDEPKKGPPAQSENPEAP
                     AEPAPAKPAQQPASAPATPAPSPAPAKPAAPASPKAPEGRVTRKKMSPLRRKIADQLV
                     SAQQTAAILTTFNECDMSAVMALRSKLQDSFVAKHGVKLGFMSFFVKAVVEALKAVPA
                     INVRVDGDEIVQNHFHDIGVAVGTERGLIVPVLRNCDEKSFADLEKDILAYAAKAKEG
                     KIEIADLSGGVFTISNGGVYGSLLSTPILNPPQSGILGMHSIQQRPVALDGQVVIRPM
                     MYLALSYDHRVVDGKEAVTFLIRVKDCIENPARMLVGA"
     gene            4711..5682
                     /locus_tag="PLP58_10060"
     CDS             4711..5682
                     /locus_tag="PLP58_10060"
                     /inference="COORDINATES: protein motif:HMM:NF016270.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF502 domain-containing protein"
                     /protein_id="HRJ08904.1"
                     /translation="MPGTPLPGESKTAMSTPAQQASFTRHPFVWLRNKLLAGLAVALP
                     LLLTFWILQNGYHLLHGWSLPILDFFARFINEISGLTVVDTSSPTFQSVVTFVGFMIP
                     LLVFIALGVAATNVLGVRVVAAMDKLLISLPIVSFIYKSLKQVIDGFKGLGARQNFKR
                     VVYVDYPSGDMKMLGFVTGQYLDPAQGKVLAAIFIPGALSPMTGLLLVVPVEQITDAP
                     LSVEEAMKLIFSGGLVVPSTLAAPADRPDINTQPLILEDEDGGPGLEDPVSEPEVLAS
                     LPRAEDFDSGDPDILSDSEYEAAVLAGQRPRGRRFLGALSWRKKRAG"
     gene            complement(5686..6060)
                     /locus_tag="PLP58_10065"
     CDS             complement(5686..6060)
                     /locus_tag="PLP58_10065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009959135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ08905.1"
                     /translation="MIQIVFNEISAAELSRLPTQIQFQLLEALNIQPADVDDAALSRR
                     FGVLERAGKKIYRCRAGDHRIYFALADGDVRVHRVLHKNTLADFLYRSNLPGGGEDDA
                     LSQSKNFWMLIDEGASTLKQRR"
     gene            6143..6841
                     /locus_tag="PLP58_10070"
     CDS             6143..6841
                     /locus_tag="PLP58_10070"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HRJ08906.1"
                     /translation="MCKAAARGEMRFMNDLSARRPLKSRSTQWASLVSRGLLRLGVSP
                     NQVSVMSIVFAAGAARLLAGGHADCCGWAWVLAALCIQMRLLCNLMDGMLAVEGGLKT
                     PHGDLYNEFPDRLADVLILAALGYAEGTAAGIALGWLCACGALMTACIRMQGAALTGA
                     HDFSGPMAKPHRMALATVSSLVMAFLTFGGSQAAPLPWLLGVMLAGILLTIHRRLRAL
                     SRALHERAAQSSAS"
     gene            6838..7443
                     /locus_tag="PLP58_10075"
     CDS             6838..7443
                     /locus_tag="PLP58_10075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009960965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HRJ08907.1"
                     /translation="MKRRLIAALARLVTGVRLLHAAPHGAGPAIYFANHASHLDFVVV
                     WAALPAEARELASPAAAEDYWGRTALRRKVACGIFQSVLIPREGITRENNPLDRLGAV
                     LEQGRSVIIFPEGTRRQDGEVGEFKAGLYHLAKRFPNIPLVPVHLDNLSRILPKGSLL
                     IVPLIAQAHFRAPIHWREGEPKPDFLRRARAALLDESQPES"
     gene            7448..8434
                     /locus_tag="PLP58_10080"
     CDS             7448..8434
                     /locus_tag="PLP58_10080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009960964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HRJ08908.1"
                     /translation="MDRDIQILLGAVLGILVIASVTGALLSGRARSDSARQTISNLNA
                     RIRSWWVMSFVFGAVLVLGPLFTTLLFACLSFLVMREFVTLTRTERADHEVLFWAFFV
                     ILPLHYLWVGAGWHGMATIFIPVYAFAFIPFRRVLTGETRGFLMSTARIQWALLICVY
                     AVSHMPMLLALPLKNYEGQNAKLLFFFVLTAQLSDVLQYVWGKTCGRRPVAPRVSPHK
                     TWEGTLGGIGSTVLVSTCLWWATPFNPWQAALMALLICLAGFFGGLVLSAIKRDLSAK
                     DWGGSIAGHGGYMDRMDSLCFAAPLFFHLTGFYFGTGMDPAPPEWILRVLGR"
     gene            8518..9279
                     /locus_tag="PLP58_10085"
     CDS             8518..9279
                     /locus_tag="PLP58_10085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009963582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RraA family protein"
                     /protein_id="HRJ08909.1"
                     /translation="MPDTARLLSHSDLLQLKRWNTPTIYNGWEQITRLDSGAACFNLD
                     ETRDFMPQMGPMIGYAVTLVIEPSNAAHRKANPGAGQEYRRYLASVPGPKIVVVQDLD
                     KPRVIGSAWGEVSANMHRALGCVGTITDGAIRDVDEMTNAGFKALARRFCVGHAHVHP
                     VRWGCEVEVFGRRVSPGDLIHADKHGFLVIPPEDQPRLLDAARFMDTNECETVIPAAR
                     ESAGRSMVETLAALEDSVARFGANVRGKFSREGEW"
     gene            9336..10151
                     /locus_tag="PLP58_10090"
     CDS             9336..10151
                     /locus_tag="PLP58_10090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009958208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase/citrate lyase family protein"
                     /protein_id="HRJ08910.1"
                     /translation="MRASKVLAKLRAGKVARICCCGSPVAFLPALAAHFQYDGVWVDG
                     EHRAWNPREIETMIGRHHAAGVDCVWRPPTKEKNALYRLLEDGAAGLMIPHVATADEA
                     RALVQAVKFPPLGDRGFCGGGRDAGYWIGKPADYTDAANRETFLVVQIETPQALENAE
                     SIAAVPGVDVLFLGPGDMSLRLGCTPAVHDPVMLDVQKKIAAACRAHGKAWGRPVGSA
                     DDVRGIAGLGAQFIVHGSEFSALHSHLGACASDFDSVLDGICQAAAIPAGKTC"
     gene            10214..12214
                     /locus_tag="PLP58_10095"
     CDS             10214..12214
                     /locus_tag="PLP58_10095"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009964580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HRJ08911.1"
                     /translation="MISILDPAQGSTGSGLVERMRHAFSTDGKLAASPQFEYRPQQQR
                     MAELVARALERNHALVVEAATGVGKSLAYLLPAVEHALEHRRKAIICTHTINLQEQLI
                     HKDLPIVRKLTGDFHAELLKGRGNYLCPSRLKNAFSHSGDLFSTSEAAELQLIEDWFR
                     QNPGGTLSEMDFTPGARVWSLVCSEPHACTPRRCPPGSGCMYQDVRRRMAEADVLVLN
                     HTLFFTLLASADETLAEDANFIFPRDFVILDEAHTIENIAAKAFGLHLSESNVRFELG
                     RLFNPKTRKGFFQSLGEQEGIREVCQAVGLAETFFREAEAACKFSGPYGKEYRVREPG
                     LLEDTLSLPLQRVARYAQKAGDAARSDGQKLEILDIAKRLNALRATLAAFLDQTEEGH
                     VYWAERGGGDNRVLSFHSAPIDVSPRLENIFFTGKKACVFTSATLGIGDDTALSYFRN
                     RVGAHKAEAATLESPFDFQKQMRLYLVKKMPEPASPDYSAALQEKISHFLTLSQGRAF
                     VLFTSYSQMTAQADRLEEFCEDNGWRLLVQGRGMPRHQMLSEFKKDTHSILLGTDSFW
                     TGVDVPGESLSNVIITRLPFAVPDHPVTASRLEHLEEQGLNPFTEYSVPEAILKLRQG
                     VGRLIRTRQDHGIVAILDPRLLSKPYGRAFLASLPDCPTEMV"
     gene            complement(12522..13217)
                     /gene="araD"
                     /locus_tag="PLP58_10100"
     CDS             complement(12522..13217)
                     /gene="araD"
                     /locus_tag="PLP58_10100"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018970636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase AraD"
                     /protein_id="HRJ08912.1"
                     /translation="MLEELKAEVCAANRALEHSGLARLTWGNVSGIDRASGLWCIKPS
                     GVDYSALTPDLMVLLDLDGEVVEGGLRPSSDTKTHLHLYRECPRVGGITHTHSPHATA
                     FAQAGRELPCFGTTHADHFHGTVPVVRALTPAEVAEDYEHFTGVAIVERLRELGLDPL
                     EMPAVLQLHHAPFTFGRNASESLKNSIALEVCAQMALATLALSPGLGPIPDHILEKHH
                     LRKHGPGAYYGQV"
     gene            13387..16278
                     /locus_tag="PLP58_10105"
     CDS             13387..16278
                     /locus_tag="PLP58_10105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009962048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PSD1 and planctomycete cytochrome C
                     domain-containing protein"
                     /protein_id="HRJ08913.1"
                     /translation="MRLGILISWLLPAAAWCAEDARALFTRRVWPLFQEKCLACHGQD
                     EGKIKGGLDMRAPESTLKGGDSGAASLVAGKPEQSPLYLAATRAHEEWEAMPPKEADK
                     LTPEQTEWIKQWITAGAPWPDETARREIAKANAARWSAEDGVTVRTTGALSPDWAARR
                     YKPEALWAYQPVRKPEVRGTNLQGAKNHPVDDLISLRLPREMTVAGAADARAFIRRAA
                     FDLTGLPPAPAEVEQFALDYAKDADAAVLALIERLLASPHYGERMARHWLDVARYADS
                     SGFANDYERGNAWRYRDYVVRSFNADKPYDLFIKEQIAGDEMSGRQGVGTPDSELLIA
                     TGFLRMGPWELTGMEVAKIARQRFLDDVTNSVGETFLAHSLQCARCHDHKFDPVPTHD
                     YYAMQACFATTQLAERPAPFLPEENTEGFEEKRHLEARRAEYSAMLRALDDKLLKNAL
                     QWFTEKGLDPAAWNEAVAQARAGAGENRRRGGEANIFGAARSILQKRGVPEGLYPPKL
                     AGFEPEDYGNERVARKGLERLRWELERYEPYAFSVYDGLTPQVTSVYQPVRMPQDRMR
                     AGELEQTAILGGGDPFSPTTPVRPGALSVLEATSSLEFQGTQEIKGRRLKLAEWIARA
                     DNPLTTRAIVNRVWLWHFGQAIAGNPNNFGSTGKPPSHPELLDWLAAVFVEKGWSFKE
                     LHRVIMTSAAYRRSAGIDANPPSGISREDLEKHHAIFKPRRLSAEELRDAMLSITGEL
                     NPALGGIPNRPEINLEVALQPRQVMGTFAAAWVPNPLPAQRHRRSLYALKIRGLRDPF
                     MEVFNEPGPDFSCEAREVSTVTPQVFSLFNGQHVHDRALALAHRVLGEAGDTGMADAR
                     AAVMTRVFGLCFGRAPTADEAGDCLAHWQAIEARHSGGSVARPKPPLEIRRDAVEENT
                     GEKFSFQEKLHGYAEFVPDLQPADVDARTRALAEVCLVLLNSNEFAYLH"
     gene            complement(16483..19251)
                     /locus_tag="PLP58_10110"
     CDS             complement(16483..19251)
                     /locus_tag="PLP58_10110"
                     /EC_number="1.2.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009962934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004591 - oxoglutarate dehydrogenase
                     (succinyl-transferring) activity [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase E1 component"
                     /protein_id="HRJ08914.1"
                     /translation="MNASVPFRANSALLDERYQEWKSNPASVDTSWAAFFEGFELGMA
                     ELAKRAGRSAAPDAGGAALSEEALNFRMRVTNALLMFRRLGHTAAHVDPLAAAGPEVS
                     ALTLDGLGFTAAELDHEVRTQMFREGSPMRLRVMLEELRRIYCGTSGFEFMHIVAPEV
                     RDWLLERVEKRLALPPPAAREQVDALRWLLEAETFERFLHRRFVGQKRFSVEGGESML
                     VALETILEGLPALGGKEIVMGMAHRGRLSVLANFLRKPLESLFYEFSENYVPNMVAGD
                     GDVKYHLGFETVRQTRDGHEISVMLAPNPSHLEAVDPVVEGMARARQRFLGDTASRSQ
                     VIPILVHGDAAFAGQGMMAEVLNLSQLPGYQTGGTIHIVVNNQIGFTTLPADARSSRY
                     CTDVAKMIDAPVFHVNGDCPLEVMQAAKLALEFRQTFKRDVVLDIVCYRRYGHNETDE
                     PAFTQPNMARSIAAHPSTATLYRRKLIAEGVLDEAAADALQKELEDELEDGVNSLASK
                     DDGKGGNPYEGFVVEPQPAYDYNVVDTGVSREDLARIGRKLIEAPEGFHLHPTIARRF
                     LAARGKAVETGEGIDWATAESLAFGTLLTEKYGVRLSGQDVRRGTFSQRHSVLYDTET
                     RERHIPLNNIQDDQGRFCVYNSLLSEAAVLGFDYGYSLLAPGMLICWEAQFGDFVNGA
                     QVIIDQFICSAESKWQRPSHITLLLPHGYEGQGPEHSSARLERFLQLCAGGNMQVCNF
                     STPAQYFHALRRQVHRSTRKPLVVMTPKSLLRHPRCVSRLDDMAAGTHFREVLDDEQL
                     AGSPDRVARLIFCSGKVYYDLLEFREKNKIKNAAIIRVEQLYPLNYEMLKEIVARYPR
                     AQKKWIWCQEEPRNMGAFYYIRPRLEEISNHKLRYAGRERSSSPAAGSKAIHVLEQEK
                     LVEDAFSV"
     gene            19476..20303
                     /locus_tag="PLP58_10115"
     CDS             19476..20303
                     /locus_tag="PLP58_10115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ08915.1"
                     /translation="MPKLSLPPVNKKIGSSLDRQKPAGVRRHPQRLKEVKKLKIKRWV
                     AWMCLFPLGTVTAFTLAEMFWRAVIRVEFWRSEPLVFFVTGGVVWWMAYLFRWRPLHS
                     YVIGHEVSHMLMARVFGGKIYKWDHGPAGGYVETDKSNTWISLAPYIVPLYPLIVLCA
                     HLATGLFFDTHEAVTVPAGVLAFSFKPAWLFYILLGFTWWFHATYTWKTVAMEQGDLR
                     RNGEFFSMQLIFVANLAVLLVLFLASSSTPGLGLGEMGRCWLSTAAFLWRNSLGLFW"
     gene            20361..>20637
                     /locus_tag="PLP58_10120"
     CDS             20361..>20637
                     /locus_tag="PLP58_10120"
                     /inference="COORDINATES: protein motif:HMM:NF025118.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HRJ08916.1"
                     /translation="METDPAREILTVSQLTREIREVLEGRVGSVWVEGEISNHRLQTS
                     GHQYFTLKDEGSQVSCVLFRGAARMASRLRDGDQVQVFGGVSVYEPRG"
BASE COUNT         3680 a         6726 c         6472 g         3759 t
ORIGIN      
        1 cgatttgagc cacaccgagc catccccgtg cagcgcgcct gtgaaggcga ccaccagccc
       61 gtccatccac agcttgccgc tgaggctgcc cttggccggc acgagcgcgg cactgaggaa
      121 gccaacggtg tcattgccag acggcgtctc ctcatccggc cgcagcagac cctggaacaa
      181 gggcttgggc cccaacgcgg cgaacgcccc ctgaatgaaa gggttcgcca cgaacaccgc
      241 cctcacgttg gtgtccgcct ccggcatggc aaacaccacc acattcccgt gcacctgcgt
      301 cccctccggc agacccgtcc agtggctgaa aatgtggccc gtcttcgcag ccgccgtcag
      361 cgtcatgagc gtgcccggca gcacctggct cgcctgcgga ttgcccttgg tcaaagcggt
      421 ggcgtttttc gccacggccg ccttcggcga cagcgtgccc gccttgtccg gcgcggcctc
      481 cagcgccgcc ggcaccgccc gcaccagggt cagcagataa cgccgctcga aggtgaacac
      541 ccgtgtcacc gacgtgctgt tgccgctcgg atcatagacc gtcaccacca gcgtgttatc
      601 cccgaccacc ggcaggatct gcgccgtgaa tggcaccgcc gtgggcttgc tgctcgcgcc
      661 caggtcggcc agcagcggcg cgccgccatt aagcaccacc tccacgcggt ccacagcgac
      721 attatccccc gccacacccg ccactgtcag cggcaaagcc gcgctcacca tcttccctgc
      781 gggcgtaacg agcttcaagg tgggtttttt ggtatcgggc aaaccagtgc ccgtaccctg
      841 gatggcgaag gagaacagcg gagaattttc cgcattgctg gcgatactga ccatggcgtt
      901 ggcgatgccc tcaagcgcag gattgaaagt gatgctaaaa aatgtgctgc cattgtcagg
      961 ctccaccgtg gcagtaggct gcccgaaaac gacgaaagcg ttgctgccgc tgatagccac
     1021 gcgtgggtta cctgtgagtt tgagcgtgcc ctcgccagga ttatgaatgg taaaggtgcg
     1081 actcagaata cccccaacgg ctggggtgcc gccaaaatcc gtgttatcca gcaacccagg
     1141 aatgacgtct ccattgacaa tgttcacacc gttgccgctg agggatattc gtggctttac
     1201 cccctccccc ttgatcgaga aggtaaaagg ggtttcatca tcgtcattgc tggcgatgct
     1261 gacgacggcc tcgtcagtgt cctcgctgcc cggatcgtaa gtgacggcaa aactcaccat
     1321 gccaccctca gcctccacac tcgacgaggc aggctgcgtg gtcacggtga aggcactgct
     1381 gccgctgagg gccaccggcg gcgtgccagt gagttcgaga gttgccaggc cgctgttctg
     1441 aatggtgaaa gtcctaatag tagtgtcgtc gtccacatcc accttgccaa agtcagtgtc
     1501 gtccaagttg ctcggcgtga catctccgtt ggcgatgtcg gcaccgttgc cgatcaggtt
     1561 gatctcgggc accgtgccag ttccagaaac cgcaaaaaca aaaggatttt tatcttgatc
     1621 atcgctggcg atgctcacca gggcactcac agtgtcatca atctcgggat cgaaggtaat
     1681 ggcaaaactc accgtgcctc cttccgccgc gaccgtggaa gagaggggct gtcccgacac
     1741 actgaatgca gtgtctccac tgacactcac aagcgggttg ccggtaagtt tgagttccac
     1801 catgccgctg ttgctaatgg tgaagatgtt cgtcaaagtg ccgtctgtct cttccatgcc
     1861 gttagtcact gccacattgc caaagtcagt tccgtccgac tcgctgggaa tgacgtcgtt
     1921 attggagatg ttcacacctt tcccactcac ggctatcttc gggccgtcac caacccctga
     1981 gatgacaaaa gtatacgggt tcttataata gtcatcgttg gcaatgctaa ctgtcgccgt
     2041 cgcaagtccc ggttcagacg ggtcaaaggt gatttcaaat tctgtgctgc caaaagaccc
     2101 cagcgttgca aggacgggtt gggatgtgac tctgaaggcg ctgctgccgc taagcgccac
     2161 acgcgggctg ccagtcagat gcagagtgcc aagatcaaag ttattgacgg caaagacgcg
     2221 tgtcaccgtg ccatcatcca cagcgacgtt gccaaagtct gtatggtccg tcatgcttgg
     2281 cgtggtgtcg ccgtcggcaa tctccatgcc attgccgttc acgacaatat caggaggttc
     2341 gccatagcct gaaataaaaa agaaaaaatt agcctcgtca tcgtcgttgt tgatgatcaa
     2401 tatctcggca ttgtttaagt aaggttctga tgggtcaaag gtcaccgtaa aattcaccat
     2461 gcctccagga tcgatagtga agccagtcgg cccatttatg aagtaagagg tgtcgcgggt
     2521 atgggtataa ttgagaatca ttatgtcacc aaaaagcagg tcggttccgc tgttgtgaat
     2581 ggtgaatgtg cgcgtcaccg tcccgccgtt catgtcggtg ctgccgaagt ctctgtaatc
     2641 tcccagtgac ggcgggccgg ccatattgct gatgttctgg ccgttcccac tcacagaaat
     2701 ctcagggctt tgagaatagg cgggaagtgc cagcatgaac gcagtggcgg cgagaaggaa
     2761 gcggggtggg ttcataaagg gaaggaagag gtggtgagtg gtgaattgct actggctgcc
     2821 ggatagcatc aggttcggcg catgacgtgg atcaattcgt cagccttttc gatgatttgc
     2881 tgaatgtggc tctggttgta gctaaaatcc gccgtgagtg tcaacgaagt cgcgacaaag
     2941 gatcctgggc agtgatgaga gctgtcaggc cgccagaaac gacgcacctg tccaaaatgg
     3001 gggtggtgcc cccactcgaa atgagggcgg gtgccgggag cctgggagct atggcagagc
     3061 ggggtggaaa tcatgacggc gactggtgct ggtgccgccg ggaaaaacga cttccaaggg
     3121 caaataccgg ttttctgccc gtttgttact cactttctga aaaaaattcc ggacaggggg
     3181 aacaagcgcc cgagccttcc aggtcgtgga gactcgcgac cgagcctttt cccccggcgc
     3241 cgaattggag gggaaaacgg tgtcagcgtg caactggctg tcagtaggaa atgctcatga
     3301 ggctgcggca tcggtctcct ccagaagggg atgatcgctt ccggcaccct gtccggcgcg
     3361 cttcacctcc agtcgcctga gtggccgcgc gcattcgccg cggggttcat cagcagcttt
     3421 tccgccagag attccagcgg gaccacgacg cgctgttcgc ttcgctgctt ggcaatggga
     3481 aaaggggtgc tactcttggc ggctcaattc cccccacatg gccgaaatca aaatccccgc
     3541 cctcggtgaa tccgtcaccg gtggccaaat ctccaaatgg catttcaaaa ccggcgacat
     3601 cgtcaaagcc ggcgatgtgc tgatgaccct cgaaaccgac aaagtcgccg ccgagatcac
     3661 cgctgaaacc tcgggcgagc tgagcatctc catggccgaa ggcgcggatg tcgagatcgg
     3721 cacggtggtt ggaagcatcg ccgagggtaa aaccgcgcca aaggaggaca aagacgagcc
     3781 gaaaaaaggg cccccggcgc aatctgaaaa ccccgaagcc cctgccgaac ccgccccggc
     3841 caagcctgcg cagcagcctg cgtccgcacc cgcaactccg gctccgtctc cggctccggc
     3901 caaacccgcc gcgcccgcca gccccaaggc gcccgaaggc cgtgtcaccc gcaaaaagat
     3961 gtctccgctg cgccggaaga tcgccgacca gctcgtcagc gcgcagcaga ccgccgccat
     4021 cctcaccacc ttcaacgagt gcgacatgag cgcggtgatg gccctgcgct cgaagctgca
     4081 ggacagcttt gtggcaaagc acggcgtgaa gctgggcttc atgtccttct ttgtgaaggc
     4141 cgtggtcgag gccctcaagg ctgtgcccgc catcaatgtc cgcgtggatg gcgacgagat
     4201 cgtgcagaac cactttcacg acatcggcgt ggccgtcggc accgagcgcg gcctcatcgt
     4261 ccccgtgctg cggaactgcg atgagaaatc cttcgccgac ctggagaagg acatcctcgc
     4321 ctatgcggcc aaggccaagg aggggaagat cgagatcgcc gacctcagcg gcggtgtttt
     4381 cacgatctcc aatggcggcg tctatggttc gctgctcagc acgcccatcc tgaatccccc
     4441 gcaaagcggc atcctgggca tgcacagcat ccagcagcgc ccggtggcgc tggacggtca
     4501 ggtggtgatc cgcccgatga tgtatctggc gctgagctac gaccaccgag tggtggatgg
     4561 caaggaggct gtcaccttcc tcatccgcgt gaaggactgc atcgagaatc cggcgcgcat
     4621 gctcgtggga gcgtgacttt ttctccatcc gccctttgca ttgaacggaa gtcggcgaca
     4681 aagcgactat cgtctcgtat gctttaaaaa atgcccggta ctcccctccc tggggagtcc
     4741 aagaccgcca tgtccacccc cgcccagcaa gcctcgttca cgcgccaccc cttcgtgtgg
     4801 ctgcggaaca agctgctggc cggtcttgcc gtggcgctgc ctctgctgct gacgttctgg
     4861 attttgcaga acggctacca ccttcttcat ggctggagcc tgccgatcct cgacttcttc
     4921 gcccgcttca tcaacgagat ctccggtctc accgtggtgg acacctccag ccccaccttc
     4981 cagtcggtgg tcacctttgt cggcttcatg atcccgctgc tggtgttcat cgccctcgga
     5041 gtggcggcca cgaatgtgct gggcgtgcgc gtggtcgccg ccatggacaa gctgctcatc
     5101 agcctgccca tcgtctcctt catctacaag tccctgaagc aggtcattga cggcttcaag
     5161 ggcctgggcg cgcggcagaa cttcaagcgc gtggtctatg tggactaccc ctccggcgac
     5221 atgaagatgc tcggcttcgt caccggccag tacctggatc ccgcgcaggg caaggtgctg
     5281 gcggccatct tcatccccgg agcgctcagc cccatgaccg gcctgctgct cgtcgtgccc
     5341 gtcgagcaaa tcaccgacgc gccgctcagc gtcgaggagg ccatgaagct catcttctcc
     5401 ggcggcctcg tcgtcccctc caccctcgcc gcgcctgcgg acaggccgga catcaacacc
     5461 cagcccctga tcctggaaga tgaggacggc ggccctggcc tggaagaccc agtctccgag
     5521 cccgaggtgc tggcctccct gccgcgcgcg gaggactttg actccggcga tcccgacatc
     5581 ctcagcgact ccgagtacga ggccgccgtg ctcgccggcc agcgccccag gggccgccgc
     5641 ttcctcggcg cgctctcctg gcggaaaaaa cgcgcgggct gagggtcagc gccgctgctt
     5701 gagcgtgctg gcgccctcgt caatgagcat ccagaaattc ttcgactggc tcagcgcgtc
     5761 gtcctcgccg ccgccgggca ggttgctgcg gtagagaaaa tcggccagcg tgttcttgtg
     5821 cagcacgcgg tggactcgca catcaccatc ggcgagcgcg aagtagatgc ggtgatcgcc
     5881 ggcccggcag cggtagattt tttttccggc ccgttcaagc acgccgaagc gcctgctcaa
     5941 agccgcgtca tccacatccg ccggctggat gttcagcgcc tccagcagtt ggaactgaat
     6001 ctgggtgggt agccgggaaa gctccgccgc gctgatttca ttgaagacga tctggatcac
     6061 ggtccgcaga ctaggcggtg atggaggacc gtcaagacgg cgctgtccgg tcaaggccgc
     6121 ttcagccttg aagaatccag gcttgtgcaa agccgccgcg cgtggtgaga tgcgcttcat
     6181 gaacgacctc tccgcacgcc gccccttgaa gtccaggagc acccagtggg cgtcgctcgt
     6241 gtcgcgcggg ctgctgcgcc tgggggtgag tccgaaccag gtctccgtga tgagcatcgt
     6301 cttcgccgca ggcgcggcgc ggctgctggc gggcggtcat gctgactgct gcggctgggc
     6361 ctgggtgctc gccgcgctgt gcatccagat gcgcctgctg tgcaatctga tggacggcat
     6421 gctggccgtg gagggtggtt tgaagactcc gcatggcgac ctttacaacg agtttcccga
     6481 ccgtctcgcc gacgtgctca tcctcgccgc actgggatat gcggaaggga cagcggcggg
     6541 catcgcactc ggctggctgt gcgcctgcgg ggcgctgatg acggcatgca ttcggatgca
     6601 gggcgcggcg ctcaccggcg cgcatgattt tagcgggccg atggccaagc cgcaccggat
     6661 ggcgcttgcc acggtgtcaa gcctggtcat ggcgtttcta acctttggcg gctcgcaagc
     6721 cgcgccgctt ccgtggcttc tgggtgtcat gctcgccggc atcctcctca cgatccaccg
     6781 ccggctgcgc gccctgtccc gcgccttgca cgagcgcgcc gcccagtcct ccgcatcatg
     6841 aaacgccgcc tcatcgccgc tctggccagg ctcgtcaccg gagtcaggct gctgcacgcc
     6901 gcgccgcatg gcgcgggtcc ggccatttac tttgcgaacc acgccagcca tctggatttt
     6961 gtcgtcgtct gggccgcgct gccggcggag gcgcgggagc tggcatcgcc ggcggcggcg
     7021 gaggattact gggggaggac cgcgctgcgg cgaaaagtgg cctgcggcat cttccagtcc
     7081 gtgctcatcc cgcgcgaagg catcacgcgg gagaacaacc cgctggaccg cctcggcgcg
     7141 gtgctggagc aggggcggtc ggtcatcatt ttcccggagg gcacccgccg ccaggatggc
     7201 gaggtggggg agttcaaagc ggggctctac catctggcga aacgcttccc aaacatcccg
     7261 cttgtaccgg tgcatctgga caacctcagc cgcattctgc cgaaaggctc gctgctcatc
     7321 gtgccgctca tcgcgcaggc gcatttccgc gcgcccatcc actggcgtga gggcgagcca
     7381 aagccggact ttctgaggcg cgcgcgcgcg gccctgctcg acgaatccca gcccgaatcc
     7441 tgacctcatg gaccgcgaca tccagatcct cctcggcgcc gtgctcggca tcctcgtcat
     7501 cgcctcggtg accggagcgc tgctctccgg acgggcgcgc tcggactccg cgcggcagac
     7561 gatctccaat ctgaacgcgc gcatccgctc ctggtgggtc atgtcgttcg tctttggcgc
     7621 cgtgctggtg ctcggtccgc tgttcaccac cctgcttttc gcctgcctgt cctttctggt
     7681 catgcgcgag ttcgtcacgc tcacgcgcac ggagcgcgcg gaccacgagg tgctgttttg
     7741 ggccttcttt gtcatcctgc cgctgcatta tctctgggtg ggggcgggct ggcatggcat
     7801 ggccaccatt ttcatcccgg tctatgcctt tgccttcatc cccttccgcc gcgtgctcac
     7861 cggggagacg aggggcttcc tcatgagcac cgcgcgcatc cagtgggcgc tgctcatctg
     7921 cgtctatgcg gtcagccaca tgcccatgct gctggcgctg ccgctgaaaa attacgaggg
     7981 gcagaacgcc aagctgctgt tctttttcgt gctcacggcg cagctcagcg acgtgcttca
     8041 gtatgtctgg ggcaagacct gcgggcgccg gcctgtggcg ccgcgcgtca gcccgcacaa
     8101 aacctgggaa ggcacgctcg gcggcatcgg cagcaccgtg ctcgtcagca cctgcctctg
     8161 gtgggccacg cccttcaacc cctggcaggc ggcgctcatg gccctgctca tctgcctggc
     8221 cggcttcttc ggcggcctgg tgctctccgc catcaagcgc gacctcagcg ccaaggactg
     8281 gggcggctcc atcgccgggc atggcggcta catggaccgc atggactcgc tctgctttgc
     8341 cgccccgttg tttttccacc tcaccggttt ctacttcggc acgggcatgg accccgcgcc
     8401 gccggagtgg atcctgcgcg tgctgggccg ctgaggcgcg tgctccgcgc tggtcctggc
     8461 aacaaaccag aagcactccc ttgccaccgc gcgcatccgc agcctgcttc ctcaaccatg
     8521 ccagacaccg cacgcctcct cagccacagc gacctcctgc agctcaagcg ctggaacact
     8581 cccaccatct acaacggctg ggagcagatc acccgcctcg actccggcgc ggcctgcttc
     8641 aaccttgatg aaacgcgcga cttcatgccg cagatgggac cgatgattgg ctacgcggtc
     8701 acgctggtca tcgaaccctc caacgccgct caccgcaagg caaacccagg cgccggacag
     8761 gagtaccgcc gttacctcgc gagcgttcca gggccgaaaa ttgtcgttgt gcaggatctc
     8821 gacaagccgc gcgtcatcgg ctcggcctgg ggcgaggtca gcgcgaacat gcaccgcgct
     8881 ctcggctgcg tgggcaccat caccgacggc gccatccgcg atgtggacga aatgaccaac
     8941 gccggcttca aggccctggc ccgcaggttt tgcgtgggcc acgcgcatgt gcatcccgtg
     9001 cgctggggct gcgaggtgga ggtcttcgga cgcagggttt cccctggcga tttgatccac
     9061 gccgacaagc acggattcct cgtcatcccc ccggaggacc agccccgcct gcttgatgcc
     9121 gcgcgcttca tggacacgaa cgagtgcgaa actgtcatcc ccgccgcgcg cgagagcgcg
     9181 ggtcgttcga tggtggaaac cctcgccgcg ctggaggact ccgtcgcgcg cttcggagcg
     9241 aatgttcgtg gaaagttcag ccgcgaaggg gaatggtgaa gcgcggcgcg ccttttcctt
     9301 ccggtaaatc caacgcccct cacccagaca cgctcatgag agcatccaaa gtcctcgcca
     9361 aactccgcgc cggcaaagtc gcgcgcattt gctgctgcgg ctcgcccgtc gcctttctac
     9421 ccgccctcgc cgcgcatttt cagtatgacg gcgtgtgggt ggacggcgag catcgcgcct
     9481 ggaacccgcg cgagatcgag accatgatcg ggcgccacca cgccgccggc gttgactgcg
     9541 tgtggcgtcc gcccaccaag gaaaaaaacg ctctttaccg tctgcttgag gacggcgcgg
     9601 ccggactcat gattccccat gtcgccactg cggatgaagc ccgcgcgctg gtgcaagccg
     9661 tcaagtttcc cccgcttggc gaccgcggct tctgtggagg cgggcgcgat gccggttact
     9721 ggatcggcaa gccagccgac tacactgacg cggccaaccg cgagaccttc ctcgtcgtgc
     9781 agatcgaaac gccgcaggcc ctggagaacg ccgaatccat cgccgccgtg cctggagtgg
     9841 acgtgctttt cctcggcccg ggagacatgt ccctgcgcct tggctgcaca cccgcggtgc
     9901 atgacccggt gatgcttgat gtgcagaaaa aaatcgccgc cgcctgccgc gcgcacggca
     9961 aggcatgggg ccgccctgtc ggcagcgctg atgatgtcag gggcattgcc ggtcttggcg
    10021 cgcagttcat cgtgcatggc agcgagttca gcgccctcca ttcccacctc ggtgcctgcg
    10081 cctccgattt tgactccgtg ctggacggca tctgccaggc tgccgccatt cccgccggca
    10141 agacttgctg agcctcgcat ttccggcctg ccccgccttg cgcctccgct ctgccacggc
    10201 atcatgcccg cgcatgatca gcattctcga ccccgcgcag ggctccacag gctccggcct
    10261 cgtggagcgg atgcgccacg ccttttccac cgatggaaag ctggccgcct cgccgcagtt
    10321 cgagtaccgc ccgcagcagc agcgcatggc cgagctcgtc gcccgcgcgt tggaaaggaa
    10381 ccacgccctc gtcgtcgagg ccgccaccgg cgttggcaag tctcttgcct atctcctccc
    10441 cgccgtcgag cacgcgctgg agcacaggcg caaggccatc atctgcacac acaccatcaa
    10501 cctccaggag cagctcattc ataaagacct gcccatcgtc cgcaaactca ccggcgactt
    10561 ccatgccgag ctgctcaaag gccgcggcaa ctacctctgc ccttcccggc tcaaaaacgc
    10621 cttctcccac agcggcgatc tcttcagcac cagcgaggcc gccgagcttc aactcatcga
    10681 ggactggttc cgccaaaatc ccggcggcac cctcagcgag atggacttca cacccggcgc
    10741 gcgcgtctgg tccctcgtgt gcagcgagcc gcacgcctgc accccgcgcc gctgcccgcc
    10801 cggcagcggc tgtatgtatc aggatgtgcg ccgccgcatg gccgaggccg atgtcctcgt
    10861 gctgaatcac actctctttt tcaccctgct cgcctccgcc gatgagaccc tcgccgagga
    10921 tgccaacttc atcttcccgc gcgacttcgt cattctcgac gaggcccata ccatcgagaa
    10981 catcgccgcc aaagcctttg gtctccacct cagcgaatcc aacgtccgct tcgagctggg
    11041 ccgcctcttc aaccccaaga cccgcaaagg cttcttccag tccctcggcg agcaggaagg
    11101 catacgcgaa gtctgccagg ccgtcggact cgccgagacc ttttttcgcg aagccgaggc
    11161 cgcctgcaaa ttcagcggcc cctacggcaa ggaataccgc gtccgcgagc ccggcctcct
    11221 ggaggacacg ctgagcctgc cgctccagcg cgtggcgcgc tacgcgcaaa aggccggcga
    11281 tgccgccagg agcgacggcc agaaactcga aatcctcgac atcgccaaac gcctcaacgc
    11341 cctgcgcgcc accctcgccg ccttcctcga ccagaccgag gaaggccacg tttattgggc
    11401 cgagcgcggc ggcggcgaca accgcgttct ttccttccac agcgcgccca ttgatgtctc
    11461 accgcggttg gaaaacatct tcttcaccgg aaaaaaagcc tgcgtcttca ccagcgccac
    11521 tcttggcatc ggcgatgaca ccgccttgag ctacttccgc aaccgtgtcg gcgcgcacaa
    11581 agccgaggct gccacgctgg agagtccctt cgattttcag aagcagatgc ggctctacct
    11641 cgtgaaaaaa atgcccgagc ccgccagccc cgactactcg gcggcgcttc aggaaaagat
    11701 ctcccatttt ctcaccctat cccaggggcg cgctttcgtg ctcttcacca gctacagcca
    11761 gatgaccgcg caggcagacc gcctggagga gttctgcgag gacaacggct ggcgcctgct
    11821 cgtccagggg cgtggcatgc cccggcacca gatgctgtcc gagttcaaaa aagacaccca
    11881 cagcatcctg ctcggcaccg acagcttttg gaccggcgtg gacgtgcccg gcgagagcct
    11941 cagcaacgtc atcatcaccc gcctgccttt cgccgtgccc gaccatcccg tcactgccag
    12001 ccggctggag cacttggagg agcagggtct caacccattc acagaataca gcgtgcccga
    12061 ggccatcctc aagctgcgcc agggcgtcgg ccgcctcatc cgcacccggc aggaccacgg
    12121 cattgtcgcc attcttgatc cccgtcttct cagcaaaccc tacggccgcg ccttcctcgc
    12181 ctccctcccg gactgcccga cggagatggt gtgaggcggg ggactgcttg gggcttccag
    12241 ggaggacggg aaatgcggcg acacgggtag gaagatactg gattatcgaa tttcacaaca
    12301 actccggaaa ccaccttggg catcgaaatt tagtgctgga gtatgggcaa atggtgtttg
    12361 cccatactca aacgccggtt gcctgtttcc agacaaggat tgtctgtgtc caaacatggg
    12421 tttctcttgt ccattttccg ggtgcctgca tccagggacc ggtggcctgt gctgcctcaa
    12481 aaacccgcca gtgcgcagca tggcagacgc cagcagcgcg ctcaaacctg cccgtaatag
    12541 gcgcccggcc cgtgtttgcg caggtggtgc ttttccagaa tatgatccgg gatgggaccc
    12601 aggccggggc ttagggccag ggtggcgagc gccatctgcg cgcacacctc gagggcgatg
    12661 ctgtttttga gggactcgga ggcgttcctg ccgaaggtga aaggcgcgtg gtgcagttgc
    12721 agcacggcgg gcatctccag cgggtccagg cccagctcgc gcaggcgctc cacgatggcc
    12781 acgccggtga agtgctcata gtcctccgcc acctcggcgg gagtcagggc gcgcaccacg
    12841 ggcacggtgc cgtgaaagtg gtcggcgtgc gtggtgccaa aacagggcag ctcgcgaccc
    12901 gcctgggcaa aggcggtggc atgaggactg tgcgtgtgcg taatgccgcc gacacgcggg
    12961 cactcgcggt aaagatgcag gtgtgttttg gtgtccgagg aggggcgcag cccgccctcg
    13021 acgacctcgc catcgagatc gagcagcacc atcaggtccg gcgtgagcgc ggagtagtcc
    13081 actccgctcg gtttgatgca ccagaggccc gaggctcggt caatgccgct gacgttgccc
    13141 caggtcaggc gcgccaggcc gctgtgctcc agggcgcggt tggcggcgca gacttctgct
    13201 ttgagttctt cgagcatggc gttgttatgc agcggtgcgc aggcggcggc aaagggaaaa
    13261 agcgcggcgg ggcgcgagtg ctggccgcgt tttcccgctc gcccccgcat ttctggcact
    13321 gcgggcgagc ggtgcgggaa tgcagtcatg gcaatctccg cccgaccgcc cgcgttatcc
    13381 tccctgatga gacttggcat cctgatttca tggctcctgc ccgccgccgc ctggtgcgcg
    13441 gaggatgcgc gcgcgctttt cacgcggcgc gtgtggccgc tcttccagga gaaatgcctg
    13501 gcctgccacg gccaggacga gggcaaaatc aaaggcgggc tggacatgcg cgcgccggag
    13561 tccacgctca agggcggcga cagcggcgcg gcatccctgg tggccggcaa accggaacaa
    13621 agccccctct atctcgccgc cacccgcgcg cacgaggagt gggaagccat gccgccgaag
    13681 gaggcggaca aactgacgcc tgagcagacg gaatggatca aacaatggat caccgcaggc
    13741 gcgccctggc ccgacgagac ggcgcgaagg gagatcgcca aggccaacgc agcgcggtgg
    13801 agcgcggagg acggcgtcac cgtcaggacc acgggcgcgc tttctcccga ctgggccgcc
    13861 aggcgttaca agccggaggc gctctgggct taccagccgg tgcgcaaacc ggaagtccgc
    13921 ggcacgaatc tccaaggcgc gaaaaaccac ccggtggatg atctgatctc cctccgtctt
    13981 ccccgggaaa tgacggtggc gggtgccgcg gacgcgcgcg ccttcatccg gcgggcggcc
    14041 tttgacctca ccggcctgcc gcccgcgccg gcggaggtgg agcagtttgc cctggattat
    14101 gcaaaggacg cggacgccgc cgtgctcgcg ctcattgaac gcctgctggc ctccccgcac
    14161 tacggggagc gcatggcgcg gcactggctg gatgtggcgc gctacgcgga cagcagcggc
    14221 tttgccaatg actacgagcg cggcaacgcc tggcgctacc gcgactatgt ggtgcgcagc
    14281 ttcaacgcgg acaagcccta cgacctgttc atcaaggagc agatcgccgg ggatgaaatg
    14341 tccggcagac agggcgtcgg gactcctgat tccgagcttc tcatcgccac cggcttcctg
    14401 cgcatgggtc cgtgggagct gaccggcatg gaggtggcaa agatcgcccg ccagcgcttc
    14461 ctcgacgatg tgaccaacag cgtgggcgag accttcctcg cgcactccct gcaatgcgcg
    14521 cgctgccacg accacaagtt cgacccggtg cccacgcatg actactacgc catgcaggcg
    14581 tgcttcgcga cgacccagct cgcagagcgc ccggctccgt ttttgccgga ggaaaacacg
    14641 gagggcttcg aggaaaaacg ccaccttgag gcgcgccgcg cggaatactc ggccatgctc
    14701 agggcgctgg atgacaagct gctgaaaaac gcgctccagt ggttcactga aaaaggcctc
    14761 gaccccgccg cttggaacga ggccgtggcg caggcgcgcg ccggggccgg tgaaaaccgc
    14821 aggcgcggcg gcgaggcgaa catcttcggc gcggcgcgct ccatcctgca aaaacgcggc
    14881 gtgccggaag gcctgtatcc gccgaagctg gccggcttcg agccggagga ctacggcaac
    14941 gagcgcgtgg cgcgcaaggg gctggagcgg ctgcgctggg agctggagcg ctacgagcct
    15001 tacgccttca gcgtctatga cggcctgaca ccgcaggtca cctccgtcta tcagccggtg
    15061 cgcatgccgc aggaccggat gcgcgcgggc gaactggagc agacggccat cctcggcggc
    15121 ggcgacccct tctcgcccac aacaccggtg aggcctggag cgctctcggt gctggaggcg
    15181 acatcaagcc tggagttcca gggtacgcag gagatcaagg gccgccgtct caagctggcg
    15241 gagtggatcg cgcgcgcgga caatccgctg acaacgcgcg ccatcgtgaa ccgcgtctgg
    15301 ctctggcact tcggccaggc catcgcgggc aatccgaaca attttggaag caccggcaag
    15361 ccgccctcgc atccggagct gctcgactgg ctggcggcgg tgtttgtcga aaaaggctgg
    15421 tcgttcaagg agctgcaccg cgtcatcatg accagcgccg cctaccgcag gagcgctgga
    15481 atagatgcaa acccgccctc cggcatctcc cgcgaggact tggagaaaca ccacgccatc
    15541 ttcaagccgc gccggctttc cgccgaggag ctgcgggacg ccatgctcag catcaccggc
    15601 gagttgaatc ccgcgctcgg cggcatcccg aaccgcccgg agatcaacct cgaagtggcg
    15661 ctccagccgc gccaggtcat gggcaccttc gccgccgcct gggtgcccaa ccccctgcct
    15721 gcgcagcgcc accgccgcag tctctacgcg ctgaaaattc gcggcttgcg cgatcccttc
    15781 atggaggtct tcaacgagcc aggccccgac ttctcctgcg aagcgcgcga agtctcgacc
    15841 gtcacaccgc aagtcttcag cctcttcaac ggccagcatg tgcatgatcg cgccctggcc
    15901 ctggcccacc gtgtcctggg cgaggccgga gacaccggca tggcggacgc gcgcgcggcg
    15961 gtgatgacaa gggtcttcgg cctttgtttc ggccgcgctc ccacagcgga tgaggcgggc
    16021 gactgcctgg cgcactggca ggccatagag gcccggcact caggcggctc cgtggcgcgg
    16081 cccaaacctc cgctcgaaat ccgccgcgat gccgtggagg agaacaccgg cgagaaattc
    16141 agcttccagg aaaaactgca cggctacgcc gaattcgtgc ccgatctcca gcccgccgat
    16201 gtggacgccc gcacccgcgc gctggccgag gtgtgcctcg ttctgctgaa ctcgaacgag
    16261 ttcgcctacc tgcactgaaa ctcacccaac gcgggcaggg ataagtcgct gattcgacca
    16321 gacacccgaa aatgaaccat gcagcgcgtc accacgattt gagcccccgg cgtactgttc
    16381 cttgggaagg ctttccgcgt ccaatcgaag gaggacgcaa tgctccaaag ctccatcact
    16441 gccgtcgcat gacatcgcca atcgctgcgg cgcgggactt gctcacacgc tgaaggcgtc
    16501 ctcgacgagt ttctcctgct ccaggacgtg gatggccttg gagccggcgg cggggctgga
    16561 gctgcgctcg cggccggcgt agcgcagctt gtggttggag atctcctcga ggcgcgggcg
    16621 gatgtagtag aaggcgccca tgttgcgcgg ctcctcctgg caccagatcc actttttctg
    16681 cgcgcgcggg tagcgcgcga cgatttcctt gagcatctcg tagttgagcg ggtaaagctg
    16741 ctcgactcgg atgatggcgg cgtttttgat cttgttcttc tcgcggaatt ccaggaggtc
    16801 ataatagacc ttgccggagc agaagatgag gcgcgcgacg cggtcagggc tgcccgcgag
    16861 ctgctcgtcg tccagcacct cgcggaagtg tgtgccggcg gccatgtcat cgagcctgga
    16921 gacgcagcgc ggatggcgca ggaggctctt tggcgtcatg acgacgagcg gcttgcgcgt
    16981 ggagcggtga acctggcggc gcagggcgtg gaaatactgg gcgggggtgg agaagttgca
    17041 cacctgcatg ttgccgccgg cgcagagctg gaggaaacgc tcgaggcggg cgctggagtg
    17101 ctcgggcccc tggccctcgt agccgtgcgg caggaggagg gtgatgtggc tgggtcgctg
    17161 ccacttgctc tcggcggagc agatgaactg gtctatgatg acctgggcgc cgttgacgaa
    17221 atccccgaac tgcgcctccc agcagatcaa catgccgggc gccagcaggg aatagccgta
    17281 gtcgaagccg agcacggcgg cctcggagag caggctgtta tagacgcaaa agcggccctg
    17341 gtcgtcctgg atgttgttga gggggatgtg acgctcgcgc gtttcggtgt cgtagagcac
    17401 gctatggcgc tggctgaagg tgccgcgccg cacgtcctgg ccggagaggc gcacgccgta
    17461 tttttcggtc aatagcgtgc cgaaggccag ggactccgcg gtggcccagt caatgccctc
    17521 gccggtttcg acggccttgc cgcgggcggc gagaaagcgg cgcgcgatgg tggggtgaag
    17581 gtggaagccc tcgggcgcct cgatgagttt tcgcccgatg cgcgccaaat cctcacggga
    17641 cacgccggtg tccacgacgt tgtagtcata ggcgggctga ggctcgacga cgaacccctc
    17701 atacggattg ccgcctttgc cgtcatcctt ggaggcaagg ctgttgacgc cgtcctccag
    17761 ctcgtcctcc agctcttttt gcagggcgtc ggcggcggcc tcgtcgagca cgccttccgc
    17821 aatgagtttg cgccggtaaa gggtggcggt ggagggatgg gcggcgatgc tgcgcgccat
    17881 gttcggctgg gtgaaggcgg gctcgtcggt ctcgttgtgg ccgtagcggc ggtagcagac
    17941 gatgtcgagc accacgtcgc gcttgaaggt ctggcgaaat tccagcgcga gcttggcggc
    18001 ctgcatgact tccagggggc agtctccgtt cacatggaag acgggggcgt caatcatctt
    18061 ggccacgtcc gtgcagtagc ggctggagcg cgcgtcggcg ggcagggtgg tgaagccgat
    18121 ctggttgttg acgacgatat ggatggtgcc gccggtctgg tagccgggga gttgggagag
    18181 gttcagcacc tcggccatca tgccctggcc ggcaaaggcg gcatccccat gcacaaggat
    18241 ggggatgacc tggctgcgcg aggccgtgtc gcccaggaag cgctggcggg cgcgggccat
    18301 gccctcgacc acagggtcca ctgcctccag gtggctgggg ttcggcgcga gcatgacgga
    18361 gatctcatgg ccgtcgcggg tctggcgcac ggtttcaaag ccgaggtggt atttcacatc
    18421 cccgtccccg gcgaccatgt tgggcacata attttccgag aactcgtaaa agaggctctc
    18481 cagcggcttg cgcaggaaat tggccagcac gctgaggcgg ccgcggtggg ccatgcccat
    18541 gacgatttcc ttgccgccga gggctggcag gccctcgagg atggtctcca gcgcgacgag
    18601 catggactcg ccgccttcga cggagaagcg cttctgcccg acaaagcggc ggtgcaggaa
    18661 gcgctcgaag gtctccgcct ccagcagcca gcgcagagcg tccacctgct cccgtgcggc
    18721 gggcggcggc agagcgaggc gtttttccac gcgctccagc agccagtcgc gcacctccgg
    18781 ggccacgatg tgcatgaatt cgaagccgct ggtgccgcag taaatccggc gcagttcctc
    18841 cagcatgacc cgcagcctca tggggctgcc ctcgcggaac atttgcgtgc gcacttcgtg
    18901 gtccagctcg gcggctgtga atcccagccc atccaaggtc agggcggaaa cctccggacc
    18961 ggcggcggca agcgggtcca catgcgcggc tgtgtgcccc agtcggcgga acatgagaag
    19021 ggcgttggtc accctcatgc ggaaattcag cgcctcctcg gagagggcgg ccccgccggc
    19081 gtcaggcgcg gcgctccttc cggcgcgctt cgccagctcc gccatgccta gctcgaatcc
    19141 ctcgaaaaat gccgcccatg aggtgtccac ggaggcgggg ttgcttttcc attcctggta
    19201 tctttcgtcg agcagggctg aattggcccg aaagggcaca gaggcgttca tgatttgggg
    19261 ggcgggggcg cagaatgatg cggtttgata cgcttgcaac ccgtgaaggg tcggaaaagc
    19321 cggtttcctg ccacgatgcc ggttttcagg attcaagggc gatttttgag gcacccagcg
    19381 cattttcacg cggatgccgg gaatgtggat aaccacgctt gttgacagat aaaatgtgaa
    19441 gaatattaac aagtcctaaa tatccaaagc tcgtgatgcc caagctgtca ctcccacccg
    19501 tcaataaaaa aatcggcagc tccctcgacc ggcagaagcc tgcgggtgtg aggcggcatc
    19561 cgcagcgcct gaaggaggtg aagaagctca aaatcaaacg ctgggtggcc tggatgtgcc
    19621 ttttccctct cggcaccgtc accgctttta cactggccga gatgttctgg cgcgcggtca
    19681 tccgggtgga attctggcgc agcgagccgc tggtgttttt cgtgaccgga ggggtcgtat
    19741 ggtggatggc gtatctgttc cgctggcgtc cgctgcactc gtatgtgatc ggccatgagg
    19801 tgagccacat gctcatggcg cgggtgttcg gcgggaaaat ctacaaatgg gaccacggcc
    19861 cggcgggcgg ctatgtcgag acggacaagt cgaacacctg gatcagcctg gcgccttaca
    19921 tcgtgccgct ttacccgctg attgtcttgt gcgcccacct ggcaaccggg ctgttttttg
    19981 acacgcatga agcggtgacg gtgcctgcgg gggtgctggc cttcagtttc aaaccggcgt
    20041 ggttgtttta catcctgctg ggcttcacct ggtggttcca cgccacctac acctggaaga
    20101 cggtggcgat ggagcagggc gatctgaggc gcaacggcga gtttttctcc atgcagttga
    20161 tctttgtcgc gaatctggcg gtgctgctgg tgctgtttct ggcgtcatcc tcgacaccgg
    20221 gcctgggact tggtgaaatg ggacgctgct ggctgtccac ggccgcgttc ctctggcgca
    20281 acagcctggg ccttttctgg tgagaagggc gcgaattccc gctggcgggg cttggcggca
    20341 tcgccaccat ggcggcgcgc atggaaaccg accccgccag ggaaatcctc accgtcagcc
    20401 agctcacccg tgaaatccgc gaggtgctcg aagggcgtgt cggcagcgtc tgggtggagg
    20461 gcgagatcag caaccaccgc ctccagacca gcggccacca atacttcacg ctcaaggacg
    20521 agggatcgca agtgtcctgc gtgctctttc gcggagcggc gaggatggcg tcaaggctca
    20581 gggatggcga ccaggtccaa gtcttcggcg gagtctccgt ctatgagccg cgcgggc
//
LOCUS       DAOMYJ010000079        20163 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Prosthecobacter sp. isolate AMR_MDS_5629
            NODE_930_length_20163_cov_21.087677, whole genome shotgun sequence.
ACCESSION   DAOMYJ010000079 DAOMYJ010000000
VERSION     DAOMYJ010000079.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867850
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prosthecobacter sp. (wastewater metagenome)
  ORGANISM  Prosthecobacter sp.
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Verrucomicrobiaceae; Prosthecobacter.
REFERENCE   1  (bases 1 to 20163)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 20163)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.30
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:34:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,742
            CDSs (total)                      :: 4,684
            Genes (coding)                    :: 4,676
            CDSs (with protein)               :: 4,676
            Genes (RNA)                       :: 58
            tRNAs                             :: 55
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20163
                     /organism="Prosthecobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_930_length_20163_cov_21.087677"
                     /isolate="AMR_MDS_5629"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1965333"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..451
                     /locus_tag="PLP58_10125"
     CDS             <1..451
                     /locus_tag="PLP58_10125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ08917.1"
                     /translation="ALLQMQCSLELLHAVEPDLDLKAQIHTHMLHVRDLAAKRFKSVL
                     AQIAKKSPEEMKMLGPDWRTVPEWKNQKGYPNPQWGPFREIWHLTREAGESALILLMV
                     DAPALTPERRTELQNLLGSFDYLHNASCGLVYHLAAYWKARRHHLIP"
     gene            448..1119
                     /locus_tag="PLP58_10130"
     CDS             448..1119
                     /locus_tag="PLP58_10130"
                     /inference="COORDINATES: protein motif:HMM:NF018178.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1080 domain-containing protein"
                     /protein_id="HRJ08918.1"
                     /translation="MKTIFNALFFASSLALAADPTPPVTQTCQPDKLIAAEAFDKPES
                     FAKEKTPGTDGWRGGIGTWSIKDGAAYAVQEGPSEKRPNGHEAVCEHIVDLGDYVLTG
                     EFKLGPSPQIGFVCRDTNKPNLHQGRVVITPTAIWIQKMSGIAKETRREELKKIEVKL
                     DPNTWHRITIEICGDRFLAKIDDHVLEASHERFKERKGRIGFVARAEGAQFRNIALWS
                     AKPKP"
     gene            1243..1617
                     /locus_tag="PLP58_10135"
     CDS             1243..1617
                     /locus_tag="PLP58_10135"
                     /inference="COORDINATES: protein motif:HMM:NF024865.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HRJ08919.1"
                     /translation="MNHATALSAYVEALNTHAWEKIAPHVAEDAVFIFTEGTFTGHTA
                     AKAAFEKTFKLIENEVFSLHDIAWTSVTENMASCHYEFRWQGLIAGQEASGGGRGTTI
                     LRKVDDRWLIAHEHLGPYARKS"
     gene            complement(1622..3463)
                     /locus_tag="PLP58_10140"
     CDS             complement(1622..3463)
                     /locus_tag="PLP58_10140"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialidase family protein"
                     /protein_id="HRJ08920.1"
                     /translation="MKHFQPRAIWISSVFSSFAMMAACAAEPPRMLFLEDAGHDATAP
                     SGTRSGYAILRREDGSFCFYNPYAPSAEPLSLPDAKGEKHTLRPALPEFIAKSKPLIE
                     SGILNNAQTLLAADGTVRSITVKAERHSKEDAARIGLPLYLDVWYQQGNATAVSEPMR
                     TWRGYNGSQMEYQRLASGRLLVPHGSYLPHAKAVPPTGRHETVIEYSDDDGASWLLSA
                     SKLTSPCYEGYNGSNEGACEPCFEELRDGSIWMLMRTQAGCLYESISKDNGTTWSKTA
                     PTRFRTSTGPANLLRHRDGRLVLSWNNCELPPKHEGIGVYGGRDALHIAISSDDGRTW
                     QGFREIYLDHRRNDNPAASGDRGTAYPLAAYTADGQIVVLAGQGKGGRNPILVDPDWI
                     TATSAESDFSDGLAQWTTYQHIGPAKRWWRGRRIGCELIETPGEKDTRSLHVRHSAST
                     DAPSEPDTAVWNFPNGWRGELTARIRLPAGSEGAIFSLNDRFFDPSNTLGEDAAVFQA
                     RVTPENTQPDTWHTLSLRWDLGRGECEYRLDEQLISTLPLRTRTLNGVSYVRIRSTAS
                     TPDPHGIIIRRVKAQISDPKAPAVPSEEVAAWQQQYVKTIVQSWADK"
     gene            3574..4809
                     /locus_tag="PLP58_10145"
     CDS             3574..4809
                     /locus_tag="PLP58_10145"
                     /inference="COORDINATES: protein motif:HMM:NF024753.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HRJ08921.1"
                     /translation="MRILFLICLALSETAASDWPRWRGPTGDGRWNPHGVPLDLARHE
                     PGRLWSAETGGGFSGVTVAAGRVCLMDRPKTPAGTERVLCFSADTGALLWGHAWEADY
                     GSMEYASGPRASVTLREGRAYALGATGVACCLDAATGAVIWQVDTVASHGAKIPTWGF
                     AASPVLDGPRVLLHVGAQPDGSVLALDRETGALVWRGGPDRAGYCTPEIITHQGARQL
                     IIWGPEMVQSLNPDTGATLWTWPYKITYGVSIAQPWYHDGILVVSGYWHGTRALKLGG
                     QAGAVSLLWENEKDMCGLMSAPLFKDGVLYLLDKNKGLQAIEHATGRLLWSDANTLTP
                     KDRNPQMSLAWLKESESLAALLNANGELVYARLGPEGIDELARHQIIGKTWAHPAFAG
                     NRVYARSDTELVAWRLWPD"
     gene            4930..6003
                     /gene="recA"
                     /locus_tag="PLP58_10150"
     CDS             4930..6003
                     /gene="recA"
                     /locus_tag="PLP58_10150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009965965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008094 - ATP-dependent activity, acting
                     on DNA [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HRJ08922.1"
                     /translation="MAKSPSKDTAADAGTQRLAEARARNLDMAIQQIQKDFGEGSILR
                     MTGDQKIEVAVIPTGNLLIDKALGVGGFARGRIIEVYGPESSGKTTLTLTAIAQAQKA
                     GGLAAFIDVEHALDPNYARRLGVKIDELLVSQPSSGEEALRICETLVRSNALDVIVVD
                     SVAALVTRQELDGEIGDSTVGAQARLMSAALRKLTAIISKARTCVIFTNQIREKIGVM
                     FGNPETTPGGKALKFYASVRVDIRRIGAIKSSDGTVTGNRTKVKIVKNKLAPPYTEAE
                     FDIMYNEGISNVGSLLDLAMEFDILQKRGSWISYKGSQLAQGRDAAKETLKNDPAIYA
                     EIEEAVKAKLAEKGLPEGSPPEA"
     gene            6122..8638
                     /gene="mutS"
                     /locus_tag="PLP58_10155"
     CDS             6122..8638
                     /gene="mutS"
                     /locus_tag="PLP58_10155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009958798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HRJ08923.1"
                     /translation="MSADAASTPMMKQYLAMRRELPEDVLLFFRLGDFYEMFFEDAKT
                     ASPILNVALTKRGGVPMCGVPYHAAQGYIAKLIKAGKRVAIGEQTSIPVPGKIVERAI
                     SQILSAGTINDTNLLDSNRPNYLAAVFRDAKSKKIGLAYVDHTTGEFEVAEYKDATEL
                     ADELERLQASEILHSDDQRDVIEALGSPACAQAVESYLFFLDQAEHSLTQHFKVQSLD
                     GFGCQGLSAALCAAGCILQYLQFQLRRSVDHIQRLRVAQTSDVVLIDAASQSHLELVS
                     ARGGNAHTLLSALDRTCTPMGGRKLRHWVLHPLRDLDTLTQRQDMIAAFLDEAMLLSQ
                     VRTLLKEVRDLERTSSRLSQGSGNGRDLQALAVSLEVIPAIKSLLSDWSTRSILSAHL
                     AHRLHDLTALSSEIQRAIVDEPPMQIKEGGVFREGWLPELDELRNASTLGRQWIADLQ
                     AREIERTGIKSLKIKYNAVFGYFIEITKANVGSVPADYHRKQTTVNGERYITDELKRM
                     EDKILGSEERSKALEYEQFIELRGRVLQHLAQIQETAETIAVLDSLASLAETARLFNY
                     TRPVLNETRNLFIRDGRHPVLDQNLTSGERFVPNDITLEPEESRLILITGPNMAGKST
                     YLRMTALLTLMAQIGSFLPVASAEIGLVDRIFTRIGASDDIARGQSTFMVEMNETALI
                     LNNATERSLVILDEIGRGTSTFDGLSIAWSVAEHLHDKVGARTLFATHYHEITALAQS
                     RTAVKNYNVAVKEWNHQIIFLHKILPGSAEKSYGIQVARLAGLSENVIARAKEVLQQL
                     ESGHQPAESVKEVPVATSVPAKTRKKPRSDEDAGQMSLFG"
     gene            8773..9831
                     /locus_tag="PLP58_10160"
     CDS             8773..9831
                     /locus_tag="PLP58_10160"
                     /inference="COORDINATES: protein motif:HMM:NF018684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1311 domain-containing protein"
                     /protein_id="HRJ08924.1"
                     /translation="MSKSEERTDWFGDKYTVHFDDNGKKIGESREEKDWFGDKYTSHT
                     DANGKKVGESREEKGWFGDKYTSHTDANGKKVGESREEKGWFGDKYTRHADADGKKVG
                     ESHEEKDWFGDKYTRHSGAVPYVARDVSKSAPASDSAATTDDNSSSSYDDDFSSYSSS
                     DSSSGNTGSGTSSIIGIMVVVAVLLGGFIVLHSDPHATNISRPTLPQTTKPTSIAPSW
                     SQPGEVESKLLERAHAEIDHVYKTVMDTLIPAQQESLRQEEREWISWRDAEADRIARR
                     TSVGGSAYRVDRLNAMIDLVKKSGRSICAAIVPSQPFERRNWAISNHLLELPFERRFW
                     TQSELPQRAIWDSLWCFR"
     gene            10208..11497
                     /locus_tag="PLP58_10165"
     CDS             10208..11497
                     /locus_tag="PLP58_10165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009959719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1552 domain-containing protein"
                     /protein_id="HRJ08925.1"
                     /translation="MKTNPLTRRHFLRAAGTVIALPALESIGFRRFASAAPTVARPKR
                     MVFLGFGWGVTEESWYPDAKVTGADYKLPPGLAPLARHQKDFTLVQGLTNKFANEAHW
                     GSTFWLTGANRFAQPGQSFHNTISADQVAAEVLGKDTRFTSIQLGSPNAIENGHGPGL
                     SLAWDRRGKPMAGLDNPVLAYHRLFSDETMPLAQRQAMLSQKRSVLDAVLTDARRVQR
                     GLNKTDTDKLDEYFQGVRDIETRLSKDEQWLDVPKPKVPLAEPKPGLAGRDEIALMYD
                     LIVAALQTDATRVITYRQPVGSLLSSLDLKIAAHDMSHYTTGDRLEASQKRDVTQSTL
                     LAGLLDKLKAVEEPDGSSLFDHTALAYGSNIRTSHYLDNCPTILAGGGASLKLGQHVV
                     LPPNTPLCNAWLTMLHGLGIQAERHGDSSGVVKELLA"
     gene            11521..13095
                     /locus_tag="PLP58_10170"
     CDS             11521..13095
                     /locus_tag="PLP58_10170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ08926.1"
                     /translation="MIRFLALFSLSSLLCAAAPLPIMQNLDGDLSFTGSTPEESKAKL
                     EKLIDDIANGPATSLMWSIGAGSDILYYQTKVASTWGWRRTKYHDDPKWGPRIARCQA
                     ATEAGLDAPRIAGEKAKQRGLSFYPSYRMNDSHYCSDPLHYPLTGRFWMEHQHTSLGA
                     SPIPAVESYKHLLNYASEEVRAYRLAVIMEAMERYADVMDGFELDFNRFQIFFPPGTA
                     ETNAPLMTALVEAVQRQLKTTAAAQGRPMRLYVRVPPAIKNCTWAGLDVVSWMKRGLI
                     NAIIPAQVMTLSHDMPLEEFVVHARPAGIEVIGSLYGRAGYHWPFAAEHGPASYSSEV
                     SRKPSAPQFLGAALNQRHQGVSGHQFYNYILHTDPATCDALSTLDGTRRHQVTTSYFH
                     DREDTYEYRKQLPTPLEVGRAAALRLLIGEDASTATASLRLGLHGANQAYSDVALTVR
                     INGHTLHKGSAASFIVVTSGKRHGIAPSPATEAYAQWPVPVELLKHGWNELAITLTAA
                     DKPLQLVETEIAVVPH"
     gene            13100..15739
                     /locus_tag="PLP58_10175"
     CDS             13100..15739
                     /locus_tag="PLP58_10175"
                     /inference="COORDINATES: protein motif:HMM:NF019259.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1588 domain-containing protein"
                     /protein_id="HRJ08927.1"
                     /translation="MKRSLQSSIAILAVSCALSDAAELPRTQFDEKHRDFFKDYCAGC
                     HNEETQKGKLRLDDIAFGIDTLESAERWQNILNALNSGEMPPEDETQPDKARKTEFLD
                     DLAGTLVIARKVLGDSGGQITMRRLNRREYKNTIRDLLGVELDVRDLPADGGTGSFDT
                     VGASLFMSGDQIEQYHALGRRALDEHFARTAAAKETFKARTEAEVLGNQRMNNNLKRL
                     KDGHEQYAAWMAAVEEAAKLPENVKLAAELRESTKIPSMNPYDGRRVGLLFNNKWEVL
                     KGAPAPQTFGLGDAETARVQELEHIYQFQYFTDYMSLPARDTGAWLGFYFAWREAYVA
                     ADAKWPPGRYTLRMRVAANEAAPAERRFIEIGQRTPDPRDVATFTVFGAHQVTGTLEN
                     PQIIETEVNVSADSFREFALREKRPNSRAAEMGIFHDHFAKTGTGPIPAVWVDWFEIE
                     GPVQAKTAAVSFALKSQDRAGVREVIERFATRAFRSQKPDAEYLDKLLLLAETRRKAG
                     DSLEDALKEAMSVVLSSPGFLYLNEPVPEQAPRQLSPLELATRLSYFLWSAPPDDELL
                     KADLTKPEVLAAQVDRLIASEKADEFVGGFAHQWLGLDRLDFFQFDFRLHRAFDDSAK
                     AAAREEVHQTLATLLRENLSLRRLLKSDFIVINGLLADYYDLDGITGDAFRKVTLPAG
                     SPRGGLLGMAAILAMGGNGKHTSPVERGAWVLRKLLHDPPPPAPPNVPQLARLEGKPL
                     TARERILAHQEEPQCASCHRKIDPIGFGLENFDAAGKWRTEDHFEKGKVKKTWPIDPA
                     AAFHNGPAFKDYFELRDLITARSDAFARGFTESLIEYALGRPFGFTDEYLAQSIISRA
                     KAKDFVLREFIHALVSSDAFQRK"
     gene            15776..17911
                     /locus_tag="PLP58_10180"
     CDS             15776..17911
                     /locus_tag="PLP58_10180"
                     /inference="COORDINATES: protein motif:HMM:NF019223.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1549 domain-containing protein"
                     /protein_id="HRJ08928.1"
                     /translation="MNTRLIPCLAMLLIFVESHAAKVSDEAAQIDALLATHWQKHGVQ
                     PNPPATDEIFVRRVHLDLAGRIPTHRETVDFLADSAADKRARLIDRLLASEGFTMHQF
                     HFYADLFRVLSKGTYQGDAGRVTGLAYAEFMKDAARKNKPFDQLARELVTAQGVAWRN
                     GAVGYYQRDRGMPLDNLAITLRVFMGTRIECAQCHNHPFDSWTQMQFHQMAAYTYGVQ
                     TVMGVYSPAFNGMFTLNRERRERTGGSLDEEAHLRQAFNEMIAPIKHAWVSHSPQRLR
                     LPHDYQYTDAKPKDLVQPAVLMGKAVPYDAKGKPLDSFAEWLTSPENPRFTTVIANRL
                     WRKMFGAGLIEPVDEIMTSTVASQPALMEHLRRLMIVQHYDIKAFLRVIANTQAYQRE
                     VTRAELMPGELYHFTGPLLRRMTAEQMWDSLATLIHTAPDLPNVPQREEKDRFLTNSR
                     KLGDALENLTPAELLERADRTSEVFKTNAAEFKKLQQQIAEARKRDDKAAVAVFAREL
                     GELRQTEIATADAHIYQPAVMKLSQHSRQTGTSYKDIIVPGYEPHDFSTEKAAQTRHF
                     LDEAARLSIPTGQHAAYVKHREAMMRLWPRAAELDSPAPNGHPLREFGQSDREFVENA
                     NSDASVPQALVLMNGQMNTHILSRWSQVMLAIKQAKYPEDRLEAAYLALFSRKPTSEE
                     KARWSATEANTAEDLLHALINTRQFIFVQ"
     gene            17964..18377
                     /locus_tag="PLP58_10185"
     CDS             17964..18377
                     /locus_tag="PLP58_10185"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HRJ08929.1"
                     /translation="MALRFLVIALLSAFVIPHSSFAAQPLNVLFIAVDDLRQEIGCYG
                     AEHIKTPHLDQLAASGMLFERAYCQVAVCNPSRNSVLSGTRPDTTGIFDKVNKEQSVC
                     PEWHGIKAGFRRKRLISAVRQAGMRSSETLGAARF"
     gene            18523..18768
                     /locus_tag="PLP58_10190"
     CDS             18523..18768
                     /locus_tag="PLP58_10190"
                     /inference="COORDINATES: protein motif:HMM:NF013561.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family transcriptional regulator"
                     /protein_id="HRJ08930.1"
                     /translation="MSTLSLKLPDSLLLRLDRESRQRRMTKSALVRAALERELEQQPT
                     AKGASCHDLARDLAGSIKKRLPKDLATNPKHMEGFGR"
     gene            18765..19184
                     /locus_tag="PLP58_10195"
     CDS             18765..19184
                     /locus_tag="PLP58_10195"
                     /inference="COORDINATES: protein motif:HMM:NF013970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain-containing protein"
                     /protein_id="HRJ08931.1"
                     /translation="MRQTVLLDTGPLISFLAAGLGHHEWVVEQWKRLKPPMLTCEAVL
                     TEAAFLLKREGVDTDSLFALLERGVIRVALEIEDQAADLRTLMRRYRNRPMSLADACL
                     VRLSELHKDAVIFTLDDDFRIYRRHGNKVIPVLMAEN"
     gene            19318..19506
                     /locus_tag="PLP58_10200"
     CDS             19318..19506
                     /locus_tag="PLP58_10200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ08932.1"
                     /translation="MTPTLSTPVEALLREQVAKGHYPSLDRALEVAVNAVDGRRASHA
                     LESLLHEALPPPGDRVPL"
     gene            19640..19765
                     /locus_tag="PLP58_10205"
     CDS             19640..19765
                     /locus_tag="PLP58_10205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ08933.1"
                     /translation="MDELHRCSHNSTFHMSRWTKKTWCEDENHVLQDVLDEVEDA"
BASE COUNT         4199 a         6465 c         5897 g         3602 t
ORIGIN      
        1 cgcactgctc cagatgcaat gctcgctgga gcttctccat gccgttgagc ctgatttgga
       61 cctcaaagcg caaatccaca cgcacatgct tcatgtgcga gacctcgccg cgaagcggtt
      121 caagagtgtt ttggctcaga tcgcgaaaaa gagccccgaa gagatgaaaa tgctcggccc
      181 cgactggcga acggttccgg agtggaagaa ccaaaaaggc tacccaaatc cgcagtgggg
      241 cccgtttcgc gagatctggc acctcacgcg tgaagcgggc gaatcggcgc tcattttatt
      301 gatggtcgat gctccagcac tcacgcccga acggcgaacc gagctgcaaa atctgctcgg
      361 cagctttgat tacctgcaca acgcaagttg cggtctcgtt taccatctcg cggcctattg
      421 gaaggcgcgc cgacatcatc tcatcccatg aagaccatct tcaacgccct tttctttgct
      481 tcttcactag ccttggccgc tgatccaacc ccgcccgtca cgcagacctg ccaaccggac
      541 aaactcatcg ccgccgaagc ctttgataag ccggaatcct tcgccaaaga gaaaacaccc
      601 ggcaccgacg gttggcgtgg cggcatcggc acctggagca tcaaagacgg cgcggcctat
      661 gccgtgcagg aaggtccgag cgagaagcgc ccgaacggcc acgaggcggt gtgcgagcac
      721 atcgtggacc tgggtgatta tgtgctgacc ggcgagttca agctcggccc ctcgccgcag
      781 atcgggtttg tctgccgtga cacgaacaaa ccgaacctcc atcaaggccg cgtggtcatc
      841 acacccacgg ccatctggat tcaaaagatg agcggtatcg ccaaagagac gcgccgcgag
      901 gaactcaaga agatcgaggt caaactcgat ccgaacacct ggcaccgcat caccatcgaa
      961 atctgcggcg accgcttcct cgccaaaatc gacgaccatg tgctcgaagc cagccacgag
     1021 cgcttcaaag agcgcaaagg ccgcatcggc ttcgtcgcac gcgctgaagg cgcgcagttt
     1081 cgaaatatcg ccctctggag cgcgaagccc aaaccgtagc ttcgaatggc attcgaagcc
     1141 gaacgcccca aactattcga ttaacaatcg aatctactca tgaaagccat cctatgcgcc
     1201 ttcagttccc tgctcatccc gctttcagca gcagaaagtc ccatgaacca cgccaccgcc
     1261 ctctccgcct acgtcgaggc cctcaacacg catgcgtggg agaaaatcgc gccccatgtc
     1321 gcggaagacg cggtgttcat cttcaccgaa ggcactttca ccggccacac cgcggcgaag
     1381 gctgcttttg agaagacctt caagctgatc gaaaacgaag tcttcagcct gcacgacatc
     1441 gcttggacgt cagtgacgga aaacatggca tcctgtcatt atgagttccg ctggcaaggt
     1501 ctgatcgccg gtcaggaagc ctctggtggt ggtcgcggga ccacgattct gcggaaggtg
     1561 gatgatcgat ggctcatcgc gcatgaacac ctcgggccat atgcgaggaa gtcgtgatgg
     1621 ctcacttgtc cgcccagctc tgcacgatgg ttttcacata ctgctgctgc catgcggcga
     1681 cctcttccga tgggacggct ggcgctttgg gatcgctgat ttgagccttc acacgccgga
     1741 taatgatgcc gtgagggtcg ggcgtgctcg cggtggagcg aatgcggaca tagctgacgc
     1801 cattcagcgt gcgtgtgcgt agcgggaggg tggagatgag ttgttcgtcg agacgatact
     1861 cgcactcgcc gcgaccgaga tcccagcgga gtgaaagcgt gtgccaggtg tcgggttggg
     1921 tgttttccgg ggtcacgcga gcttgaaaca cggcagcgtc ctcgcccagc gtgttcgagg
     1981 gatcgaaaaa gcggtcgttg agcgagaaaa tcgcgccttc agagcctgct ggcaggcgga
     2041 tgcgggcggt gagttcgcct cgccagccgt tggggaagtt ccacaccgcc gtgtcaggct
     2101 cgctgggtgc gtcggtgctg gcggagtggc gcacatgcag gctgcgcgtg tctttttcgc
     2161 cgggagtttc gatgagttcg cagccgatgc gacgaccacg ccaccagcgc ttcgctgggc
     2221 cgatgtgttg ataggtcgtc cattgcgcaa ggccgtcgct gaagtcggac tcggcgctgg
     2281 tggcggtgat ccagtcggga tcgacgagga tgggattgcg cccacctttg ccctgcccgg
     2341 cgagcacgac gatctgaccg tcggctgtgt aagcagcgag cggataagcc gtgccgcggt
     2401 ctccagatgc ggcgggattg tcgttgcgtc gatgatcgag gtaaatctca cgaaaaccct
     2461 gccaggtgcg gccatcatcg gacgagatgg cgatgtgcag cgcatcgcgc ccgccataca
     2521 cgccgatgcc ttcgtgcttg ggaggcagtt cgcagttgtt ccagctcaaa acgagtcgtc
     2581 catcgcgatg acgcagcagg ttcgcaggcc cagtcgaggt gcggaagcgc gtgggtgcgg
     2641 tctttgacca agtggtgccg ttgtctttgg agatggactc ataaagacaa cccgcctgcg
     2701 tgcgcatgag catccagatc gagccatcgc gcagctcttc aaagcagggc tcgcaggcac
     2761 cttcgttgga gccgttgtag ccttcgtagc agggcgaggt gagcttcgag gcgctgagca
     2821 gccagctcgc gccgtcatcg tcggagtatt cgatcaccgt ctcatgacga cccgtcggcg
     2881 gcactgcttt cgcatgaggc agatagctgc catgcggcac cagcaggcga ccgctcgcga
     2941 ggcgctgata ctccatctgc gagccattgt agccgcgcca cgttcgcatt ggctcggaaa
     3001 cggcggtggc attcccttgc tgataccaca cgtcgagata cagcggcagg ccgatgcgtg
     3061 ccgcgtcctc tttactgtgc cgctccgctt tcaccgtgat gctgcggaca gtgccgtcgg
     3121 cggcgagcaa ggtctgcgcg ttgttcagga tgccgctttc gatgagcggc ttcgatttgg
     3181 cgatgaattc cggcaaggca ggccgcagcg tgtgcttttc acccttcgca tccggtaaac
     3241 tcagcggctc cgcgctgggc gcgtagggat tgtagaagca aaagcttcca tcctcgcggc
     3301 gcaggatcgc gtagcctgag cgtgtgccgc tgggcgcggt ggcatcatga ccggcatctt
     3361 cgaggaaaag catgcgcgga ggctctgcgg cgcacgcagc catcatggca aagctgctga
     3421 acacgctgga gatccaaatg gctcgtggct ggaagtgttt catgataagg cgcatgattc
     3481 tgaaacgcgg cggcatgata tgtcatgaag attttcatca ccggccaacc actccacgcc
     3541 agcaaagcgg agtggcgcgc gtattaccct gccatgcgaa tccttttttt gatctgcctc
     3601 gccctctccg agaccgcagc atccgactgg ccgcgctggc gtggaccgac gggcgatgga
     3661 cgctggaatc ctcacggcgt gcctctggat ctggcgcggc atgagcctgg gagattgtgg
     3721 agcgcggaaa ccggcggcgg cttcagcgga gtgaccgtgg cggcaggcag ggtgtgtttg
     3781 atggaccgcc ccaaaactcc cgccggcacg gagcgcgtgc tgtgtttctc cgcggacact
     3841 ggcgcgctgc tctggggcca tgcctgggag gcggactacg gcagcatgga atacgccagc
     3901 ggcccgcgcg cctcggtgac gctgcgcgag ggccgcgcct acgcgctcgg cgccaccggc
     3961 gtcgcctgct gcctggacgc ggccaccggc gcggtcatct ggcaggtgga cacggtggcc
     4021 agtcacggcg ccaaaattcc cacctggggt ttcgccgcct cgcccgtgct ggacgggccg
     4081 cgcgtgctgc tgcatgtggg cgcgcagccc gatggcagcg tgctcgccct tgatcgggag
     4141 accggcgcgc tggtctggcg cggcgggccg gaccgcgcgg gttattgcac gccggagatc
     4201 atcacacacc agggagcccg gcagctcatc atatggggac cggagatggt gcagagcctg
     4261 aatcccgaca cgggcgccac gctttggaca tggccgtaca aaatcaccta tggtgtcagc
     4321 atcgcgcagc cgtggtatca cgacggcatc ctcgtggtgt ccggttactg gcacggcacc
     4381 agggcgctca agctcggcgg ccaggcgggc gcggtgtcgc tgctttggga gaatgaaaaa
     4441 gacatgtgcg gtctgatgtc tgcgccgctt tttaaagacg gcgtgctata cctgctggac
     4501 aaaaacaagg gccttcaggc catcgagcac gccaccggca ggctgctatg gagcgatgcc
     4561 aacaccctca cccccaagga ccgcaacccg cagatgagcc tcgcctggct caaggaaagc
     4621 gagagtctcg ccgccctgct caatgccaat ggagagctcg tctatgcgcg gctggggccg
     4681 gagggcatcg acgagcttgc ccggcatcaa atcatcggca agacctgggc gcatcccgcc
     4741 tttgcaggga accgggttta tgcccgctca gacacggagc ttgtcgcgtg gcgtttatgg
     4801 ccggattgag gaggcggggc ttgccccgct ctcgcggagc gctttaaatc gccgcgcttt
     4861 ggaaaaaacc attgcgcgcg gcgatcacca ccctaagtgt tcaccggaac aaattaacca
     4921 ctctctctca tggccaaatc cccctccaaa gacaccgccg ccgacgccgg cacccagcgc
     4981 ctcgccgaag cccgcgcccg caacctcgac atggccatcc agcaaatcca gaaggatttc
     5041 ggcgaagggt ccatcctgcg catgacaggc gaccaaaaaa tcgaggtggc cgtcatcccc
     5101 accggcaacc ttttgataga caaggccctg ggcgtgggcg gtttcgcccg cgggcgcatc
     5161 atcgaggttt acgggccgga atcctccggc aaaaccaccc tcacgctcac cgccatcgcc
     5221 caggcgcaaa aggccggcgg cctggccgcc ttcattgacg tggagcacgc gctggatccg
     5281 aactacgcgc gccgtctcgg tgtgaaaatt gacgagctgc tcgtctccca gcccagttcc
     5341 ggcgaggagg cgctgcgcat ctgcgagacc ctggtgcgct cgaacgccct tgacgtcatc
     5401 gtcgtggact ccgtggccgc tctggtcacc cgccaggaac tcgacggcga gatcggcgac
     5461 tccaccgtcg gcgcgcaggc ccgcctcatg agcgccgccc tgcgcaagct caccgccatc
     5521 atctccaagg cccgcacctg cgtcatcttc accaaccaga ttcgtgaaaa aatcggcgtc
     5581 atgtttggca accccgaaac cacgcccggc ggcaaggccc tcaaattcta cgccagcgtg
     5641 cgcgtggaca tccgccgcat cggcgccatc aaaagcagcg acggcaccgt caccggcaac
     5701 cgcaccaagg tcaaaatcgt caaaaacaag ctcgccccgc cctacaccga ggccgagttc
     5761 gacatcatgt acaacgaggg catctccaat gtcggctccc tgctcgacct ggccatggag
     5821 ttcgacatcc tgcaaaaacg cggctcctgg atcagctaca agggcagcca actcgcccag
     5881 gggcgcgacg ccgccaagga aacgctgaaa aacgaccctg ccatctatgc ggagatcgag
     5941 gaggcggtga aggccaaact cgccgagaag ggcctgcccg aaggcagccc gcccgaggca
     6001 taaaccgccg ccgctgccgc aacgcggcgg tcattttcat cccgccccgg cttgatgatg
     6061 ccagactggg cgaagaaggc ttgaccgccg cccgcatgac ggccaagcct tgcgtttcac
     6121 gatgtccgcc gacgccgcca gcaccccgat gatgaagcaa tacctcgcca tgcggcggga
     6181 gcttcccgag gatgtgctgc tctttttccg cttgggtgac ttttacgaga tgttcttcga
     6241 ggacgcgaag accgcctccc ccatcctcaa tgtggcgctc accaagcgcg gcggagtgcc
     6301 catgtgcggc gtgccttacc atgcggcgca gggttacatc gccaagctca tcaaagccgg
     6361 caagcgcgtc gccatcggcg agcagaccag catccccgtg ccgggaaaaa tcgtcgagcg
     6421 ggccatctcg cagattctca gcgcgggcac catcaacgac acgaacctgc tcgactccaa
     6481 ccggccgaac tacctcgccg cggtctttcg cgatgcaaaa agcaaaaaga tcggcctcgc
     6541 gtatgtggac cacaccaccg gcgagttcga ggtggcggaa tacaaggacg ccaccgagct
     6601 cgccgatgaa ctggagcgcc tccaggccag cgagatcctc cacagcgacg atcagcgcga
     6661 tgtgatcgag gcgctcggca gcccggcctg tgcgcaggcg gtggagagct acctgttttt
     6721 tctcgatcag gctgagcaca gcctcacgca gcactttaaa gtgcagtcgc tcgatggatt
     6781 cggctgccag gggctcagcg ccgcgctttg tgccgcaggc tgcatcctgc aatacctgca
     6841 attccagctc cgccgcagtg tcgatcacat ccagcgcctc cgcgtcgcgc aaacgagcga
     6901 cgtcgtgctc atcgacgccg ccagccagag ccatcttgaa ctcgtcagcg cccgcggcgg
     6961 caatgcccac acgcttttga gcgccctcga ccgcacctgc acccccatgg gcggccgcaa
     7021 gctccgccac tgggtcctcc acccgctgcg cgatctcgac acgctcacgc aacgccagga
     7081 catgatcgcc gcttttctcg atgaggccat gctcctcagc caggtgcgca ccctgctcaa
     7141 agaagtgcgt gatctggaac gcaccagcag ccgcctcagc caaggcagcg gcaatggccg
     7201 tgatctgcag gcgttggcgg tgtcgttgga ggtcattccc gccatcaaat cgcttctgtc
     7261 agactggtca acccggtcaa tcctgtcagc ccacctcgcc catcgcctcc acgacctcac
     7321 cgcgctctct tccgaaatcc aacgcgccat cgtcgatgaa ccgccgatgc agatcaaaga
     7381 aggcggcgtg ttccgcgaag gctggctgcc ggagctcgac gagctccgca atgcctccac
     7441 gctcggccgg cagtggatcg cggatttgca ggcgcgggag atcgagcgca cgggcatcaa
     7501 gagcctcaag atcaaataca acgccgtctt cggctacttc atcgagatca ccaaggcgaa
     7561 cgtcggcagc gtgcccgccg actaccaccg caagcagacc accgtcaacg gcgagcgcta
     7621 catcaccgac gagttgaagc gcatggagga caagatcctc ggcagcgagg agcgcagcaa
     7681 ggcgctcgaa tacgagcagt tcatcgagct gcgcggccgc gtgctgcagc acctcgcgca
     7741 gatccaggag accgcggaga ccatcgccgt gctcgattca ctcgcctcgc tggccgagac
     7801 cgcgcggctc ttcaattaca cgcgccccgt gctcaatgaa acgcggaatc tcttcatccg
     7861 cgacggccgc catcccgtgc tcgatcagaa tctcacaagt ggcgagcgct tcgtgccgaa
     7921 tgacatcacg ctggagcccg aggagagccg cctcatcctc atcaccggcc cgaacatggc
     7981 cggtaagagc acctacttgc gcatgaccgc gcttctcacg ctcatggcgc agattggcag
     8041 cttcctgccc gtcgccagcg cggagatcgg cctcgtcgat cgcatcttca cccgcatcgg
     8101 cgccagcgac gacatcgcgc gcggccagag caccttcatg gtcgagatga acgaaaccgc
     8161 gctcatcctc aacaacgcca ccgagcgctc cttggtcatc ctcgacgaga tcggccgcgg
     8221 cacgagcacc ttcgacggcc tcagcatcgc gtggagcgtc gccgagcatc tgcacgacaa
     8281 agtcggcgca cgcacgctct tcgccacgca ctaccacgag atcaccgcgc tcgcacagag
     8341 ccgcacggcc gtgaagaact acaacgtcgc cgtgaaggag tggaaccatc agatcatctt
     8401 cctccacaag atcctccccg gctccgccga aaaaagctac ggcatccagg tcgcgagact
     8461 cgccggcctc tccgaaaacg tcatcgcccg cgccaaggaa gtcctccaac aactcgaatc
     8521 cggccaccag cccgccgaaa gcgtcaaaga agtccccgtg gccaccagcg tgcccgcgaa
     8581 gacaaggaag aagccccgca gcgatgaaga cgcggggcag atgagtttgt ttgggtgaaa
     8641 gtgagggttg ccaaatccat ctcgaacaga cattttgcca ctaaatcgtg ggtgatgatt
     8701 aagctgctta aaaagctcgc gttctgttaa ccatctgcgc tgttggcgcg ttcactgtta
     8761 tctcacaata ccatgagtaa atctgaggaa agaacagact ggtttggtga taaatacacc
     8821 gtccattttg atgataatgg aaaaaagatc ggtgaatctc gcgaggaaaa agattggttt
     8881 ggcgataaat acacaagtca cactgacgcc aatggaaaaa aagtcggtga atctcgcgag
     8941 gaaaaaggtt ggttcggcga taaatacaca agtcacactg acgccaatgg aaaaaaggtc
     9001 ggtgaatctc gcgaggaaaa aggttggttc ggcgataaat acacaagaca cgctgatgcg
     9061 gatggaaaaa aggtcggtga gtctcacgaa gaaaaagatt ggttcggcga taaatacaca
     9121 aggcactcag gggctgttcc ttatgtggct cgagacgttt ccaaatcagc tcccgcaagt
     9181 gattctgccg ccacaaccga cgacaattcc tcttcgagct acgacgacga tttcagctct
     9241 tattcgagta gcgattcgag cagtggcaac actggctccg gcacaagcag catcattgga
     9301 atcatggtcg tagtagctgt cttgctggga ggatttattg ttctgcactc agatccgcac
     9361 gcgacaaaca tatccagacc aaccctccca caaacgacca aaccaacatc tatagctccg
     9421 tcttggagcc aacccggcga ggtagaatcg aagctgctgg agcgtgctca tgctgaaatc
     9481 gaccatgttt acaagacagt gatggatact ttaatccctg ctcagcaaga gtcgcttcgc
     9541 caagaagaac gcgaatggat aagctggcgg gacgcagagg cagacagaat cgcccgaaga
     9601 acgtctgtcg gcggaagcgc ctatcgcgta gatcggctta atgccatgat cgatctggtc
     9661 aaaaaaagcg gacggagtat ttgcgccgct atcgtcccaa gccagccgtt cgaacggagg
     9721 aactgggcta tatcgaacca ccttctggaa ctcccattcg aaaggcgatt ctggacacaa
     9781 tcagaactcc cgcagagagc tatttgggac agcctgtggt gtttcaggtg aaccgtctgc
     9841 gtgtatctgg ggcagataca gggtgtccgt ccctctatcg cgtccccgtc cctctatcgc
     9901 gtcccattcg tgtctgtccc tttgtccctt tgtccctttg tccctttgtc cctttatcct
     9961 ctttatcctg gtccctttat cctgccttta tcgctgcttc gatcccctgg agcagcacga
    10021 cctcgccgaa atgcccgagc atcgcgatac gctgaacctc atgcgcgaaa agctgctcac
    10081 gcacctccgc tccacgcaaa caactttccg ctggttacaa accaaaggtt cagcggctgc
    10141 gtgaggctga agagaagaag gaaggaaatt gatgacgcgg tgacgacgtt tccatcgcac
    10201 cgctgccatg aaaacgaatc ctctcacccg ccgccacttt ctccgcgccg ctggcacggt
    10261 catcgcgctg cccgcgctgg aatccatcgg ctttcgccgg tttgcttctg ccgcgcccac
    10321 tgtggcaagg ccgaagcgca tggtgtttct cggctttggc tggggagtga cggaggagtc
    10381 gtggtatccc gatgctaagg tgacgggcgc tgactacaag ctgccgccgg gcctcgcgcc
    10441 tttggcacgg catcaaaagg acttcacgct cgtgcagggg ctgacgaaca agttcgccaa
    10501 cgaggcgcac tggggcagca cgttctggct cacgggggcg aatcgtttcg cgcagccggg
    10561 gcagagcttt cacaacacca tctccgccga ccaggtggcg gcggaggtct tgggcaaaga
    10621 cacgcgcttc acctccatcc agctcggcag cccaaatgcg attgagaacg gtcacggtcc
    10681 cggcctctcg ctcgcctggg accgccgcgg caaaccgatg gcaggtctgg acaatcccgt
    10741 gctggcctat caccgcctgt tttccgatga aacgatgccg ctcgcgcagc gccaggcgat
    10801 gctctcacaa aagcgcagtg tgctcgatgc cgtgctcacc gacgcgcggc gtgtgcagcg
    10861 cggtctgaac aagaccgaca ctgacaagct cgatgaatat ttccaaggcg tgcgcgacat
    10921 cgagacacgt ctgtccaagg acgagcaatg gctcgacgtg cccaagccaa aggtgccgct
    10981 cgccgagccg aagccggggc tcgcaggccg tgatgagatc gcgctgatgt atgacctcat
    11041 cgtggcggcg ctccaaaccg acgccacacg tgtcatcacc taccgccagc ctgtcggatc
    11101 acttctgagc agcctggatc taaaaatcgc cgcgcacgac atgagccact acaccaccgg
    11161 cgaccgcctg gaggcctcgc aaaagcgcga tgtgacgcag agcacgctgc tcgccggctt
    11221 gcttgacaag ctcaaagcgg tggaggaacc cgacggcagc agccttttcg accacaccgc
    11281 gctcgcctac ggcagcaaca tccgcacgag ccactacctg gacaactgcc cgacgattct
    11341 cgccggcggc ggtgccagcc tgaaattggg ccagcacgtc gtcctgccgc cgaacacgcc
    11401 gctctgcaac gcctggctga cgatgctcca cggcctcggc atccaagccg agcgccacgg
    11461 cgacagctcg ggtgtggtga aggagttgct ggcgtgacat cagccgcttt ccccaatccg
    11521 atgatacgat tcctcgccct cttttcactg agttcactgc tatgtgcagc ggcaccgctg
    11581 cccatcatgc aaaacttgga tggcgacttg tcattcaccg gcagcacacc ggaggaatcg
    11641 aaggccaaac ttgagaagtt gatagatgac atcgccaacg ggcccgcaac gtcgctgatg
    11701 tggtccatcg gagctggctc ggatattctt tactaccaga ccaaagtggc gagcacctgg
    11761 ggctggcgca ggacgaagta tcacgatgat cccaaatggg gcccccggat cgcgcgctgc
    11821 caggccgcga ccgaggctgg gctggatgcg ccacgcatcg cgggtgagaa ggcgaagcag
    11881 cgtggcctct ccttttatcc ctcctaccgg atgaacgact cgcactactg tagcgacccg
    11941 ctgcactacc cgctcaccgg tcgcttctgg atggagcacc agcacaccag ccttggcgcc
    12001 tcacccatcc ctgcggtcga gagctacaag catctgctca actacgccag tgaggaggtt
    12061 cgcgcctatc ggctcgcagt catcatggag gccatggagc gttatgccga tgtgatggac
    12121 ggcttcgagc tcgacttcaa tcgcttccaa atcttcttcc cgccgggcac agcggaaaca
    12181 aacgcgccgc tgatgacagc gcttgtcgag gcggtgcagc ggcaactcaa aaccactgcc
    12241 gcagcgcagg ggcgtccgat gaggctctat gtccgcgtgc cgcctgcgat aaagaactgc
    12301 acctgggccg gactcgacgt ggtgagctgg atgaagcgtg gcctgatcaa tgccatcatc
    12361 cctgcgcagg tgatgaccct gtcccacgac atgccgttgg aggagtttgt ggttcatgcc
    12421 cgaccggcgg gcatcgaagt catcggctcg ctttacggca gggctggcta tcactggccg
    12481 ttcgcggcag agcacggccc ggcatcctac tccagcgagg tttcacgcaa acccagtgcg
    12541 ccacagttcc tcggtgcagc tctgaaccag cgtcatcagg gcgtgtccgg tcatcagttt
    12601 tacaattaca tcctgcacac cgacccggcc acctgcgacg cactgagcac gctggatggc
    12661 acccggcgtc atcaggtaac cacgagctac ttccacgatc gggaggatac atacgagtat
    12721 cgcaaacaac tccccactcc attggaagtg ggacgcgccg ccgcactgcg actgctgata
    12781 ggagaagatg catcgactgc caccgccagc cttcgcctgg ggctgcacgg agcgaatcaa
    12841 gcatactccg acgtggcgct caccgtccgc atcaacggcc acaccttgca caaaggaagc
    12901 gccgccagct tcatcgtcgt cacgtcgggg aaacgtcatg gcatcgcgcc ctcacccgcc
    12961 accgaggcct atgcacaatg gcccgtgccg gtcgagcttc tcaagcatgg ctggaacgaa
    13021 ctcgccatca cgctgaccgc cgcagacaag ccgctgcaac ttgtcgaaac tgaaatcgcc
    13081 gtcgttcctc actgagacca tgaaacggtc gctccagtca tcgattgcca tccttgcagt
    13141 ctcttgcgca ctgtcggacg ccgctgagct gccgcgtacg cagttcgatg aaaagcaccg
    13201 cgactttttc aaagactact gcgccgggtg ccacaatgag gaaacgcaaa agggcaagct
    13261 gcggctggat gacatcgcgt ttggcatcga cacgctggag agcgcggagc gctggcagaa
    13321 tatcctcaac gcgctgaact ccggcgagat gccgccggag gatgagacgc agccggacaa
    13381 ggcacggaaa acggagtttc tcgatgatct cgcgggcacg ctcgtcatcg cacgcaaggt
    13441 gctgggtgat tccggcgggc agatcaccat gcggcggctg aaccggcgcg aatacaagaa
    13501 cacgatccgc gatctgctcg gcgtggagct ggatgtgcgc gatctcccgg cggacggcgg
    13561 cacgggcagc ttcgacacgg tgggcgcgtc gctcttcatg tcgggcgatc agatcgagca
    13621 ataccacgcg ctgggccgcc gtgcgctgga cgagcacttt gcgcggactg cggcggcaaa
    13681 ggagactttc aaagcgcgaa cggaggccga ggtgctcggc aatcagcgca tgaacaacaa
    13741 cctcaagcga ctcaaggacg gccacgagca atacgcggcc tggatggccg ctgtagagga
    13801 agccgcgaag ttgccggaga atgtgaagct cgccgccgag ctgcgagaga gcacaaaaat
    13861 cccctcaatg aacccttacg atggccgacg cgttgggctg ctcttcaaca acaagtggga
    13921 ggtgctcaaa ggcgcgcccg cgccgcagac cttcggcctt ggcgatgcgg aaacggcccg
    13981 cgtgcaggag ctggagcaca tctaccagtt ccaatacttc accgactaca tgagcctgcc
    14041 cgcgcgcgac accggcgcat ggctcggctt ctacttcgcc tggcgcgagg cgtatgtggc
    14101 cgccgatgcg aagtggccgc ccggccgcta cacgctgcgc atgcgcgtgg cggcaaacga
    14161 ggccgcgccg gcggagcgcc gcttcatcga gatcggccag cgcacgcctg accccaggga
    14221 cgtcgccacc ttcaccgtgt tcggcgcgca tcaggtcacg ggcacgttgg aaaatccgca
    14281 aatcatcgag acggaggtga atgtgagcgc ggacagcttc cgcgagttcg cgctgcgtga
    14341 aaaacgccca aactcgcgcg cggcggagat gggcatcttc cacgatcact ttgccaaaac
    14401 cggcaccggc ccgatcccgg cggtgtgggt ggactggttt gagatcgagg gacctgtgca
    14461 ggcaaaaacc gccgctgttt ctttcgcgct gaaatcgcag gaccgcgccg gagtgcgcga
    14521 ggtgatcgaa agattcgcca cccgcgcctt tcgaagtcaa aagccagacg ccgagtatct
    14581 cgacaagctc ctgctgctcg ccgaaacgcg acgcaaggcg ggtgactcgc tcgaagacgc
    14641 gctgaaagag gcgatgagtg tcgtgctctc ctcgccgggc tttctttatc taaatgaacc
    14701 ggtgccagaa caggcaccac gacagctttc accactcgaa ctcgccaccc gcctctccta
    14761 cttcctctgg agcgcgccgc cggacgacga actgctcaaa gccgacctca ccaagcccga
    14821 agtgctcgcc gcgcaagtgg accgcctcat cgccagcgag aaggccgatg agttcgtcgg
    14881 cggtttcgcg catcaatggc tcggactcga ccggctcgat tttttccagt tcgacttccg
    14941 cctgcaccgc gcctttgacg acagcgccaa ggccgccgcg cgcgaggagg tgcatcagac
    15001 gctggccacg ctgctgcgtg agaacctgag cctgcggcgg ctgctgaaat ccgacttcat
    15061 cgtcatcaac ggcctgcttg ccgactacta cgacctcgat ggcatcacgg gcgatgcctt
    15121 ccgcaaggtc acgctgcccg caggctcacc acgcggcggc ctgctcggca tggccgccat
    15181 cctcgccatg ggcggcaatg gcaagcacac cagccccgtc gagcgcggcg cgtgggtgct
    15241 gcgaaagctg ctgcacgatc caccgccgcc cgcgccaccg aatgtgccgc agctcgcgcg
    15301 gctcgaaggc aaaccgctca ccgcgcgcga gcgcatcctc gctcatcaag aagagccgca
    15361 atgcgcaagc tgccatcgca agatagatcc catcggcttc ggcctcgaaa acttcgacgc
    15421 cgccggcaaa tggcgcaccg aggaccactt cgagaagggc aaagtcaaaa agacctggcc
    15481 catcgacccc gccgccgcct tccacaacgg ccccgccttc aaagactact tcgagctgcg
    15541 cgacctcatc accgcgagga gcgatgcctt cgcacgcggc ttcaccgagt ccctcatcga
    15601 atacgccctc ggacgacctt tcggcttcac ggatgaatac ctcgctcaat caatcatcag
    15661 ccgagccaag gcgaaagatt tcgtcctgcg cgagttcatt cacgcccttg tctccagcga
    15721 tgctttccaa cgcaagtgag tgagatgctg tgttcgtctc gcgttattgc gttgaatgaa
    15781 cacgcgcctc atcccctgcc tcgccatgct tctcatcttt gtcgagtctc atgccgcgaa
    15841 agtctctgat gaagccgctc agattgatgc gctgctcgcc acacactggc aaaagcacgg
    15901 tgtgcagccg aatccgcccg ccacggatga aatcttcgtc cgccgggtgc atctggatct
    15961 cgcggggcgc atcccgacgc atcgggagac ggttgatttt ctcgcggaca gcgcggcgga
    16021 caaacgcgcg cgcttgatcg accgcctgct cgccagcgag ggcttcacga tgcaccagtt
    16081 tcacttctac gcggacctgt ttcgcgtgct gagcaagggc acctaccagg gcgatgcggg
    16141 gcgtgtcacc gggctggcgt atgcggagtt catgaaagac gccgcgcgaa aaaacaaacc
    16201 ctttgaccag ctcgcgcggg agctggtcac ggcgcagggc gtggcctggc ggaacggcgc
    16261 ggtgggctat taccagcggg atcgcggcat gccgctggac aatctcgcca tcacgctgcg
    16321 cgtcttcatg ggcacgcgca tcgagtgcgc gcagtgccat aaccatccct tcgactcgtg
    16381 gacgcagatg cagttccacc agatggcggc ctacacctac ggcgtgcaga cggtgatggg
    16441 cgtctacagc ccggccttca acggcatgtt cacgctgaac cgcgagcggc gggagaggac
    16501 cggcggctcg ctggatgaag aggcgcatct gcgccaggcc ttcaatgaaa tgatcgcgcc
    16561 gatcaagcac gcatgggtca gccacagccc gcagcgcctg cgcctgccgc acgattatca
    16621 atacacagat gccaaaccga aggatctcgt gcagcccgcc gtgctcatgg gcaaggccgt
    16681 gccttatgat gcgaagggca agcctctcga ctccttcgcc gaatggctca cctcgccgga
    16741 gaatccgcgc ttcaccaccg tcatcgcgaa ccgcttgtgg aggaaaatgt tcggcgcggg
    16801 actcatcgag ccggtggacg agatcatgac cagcaccgtg gcatctcagc ccgcgctcat
    16861 ggagcatctg cggcggctca tgatcgtaca gcactatgat ataaaggcat tcctgcgcgt
    16921 catcgcgaac acgcaagcct atcagcgcga ggtcacgcgt gccgagttga tgcccggcga
    16981 gctgtatcac ttcaccggcc cgcttttgcg ccgcatgacg gcggagcaga tgtgggacag
    17041 cctcgccacg ctcatccaca ccgcgcctga tttgccgaat gtgccgcagc gtgaggaaaa
    17101 agaccgcttt ctcaccaact cccgcaaact cggtgacgcg ctcgaaaacc tcacgcccgc
    17161 cgagctgctg gaacgcgccg accgcacgag cgaagtcttc aaaacgaacg ccgccgagtt
    17221 caaaaagctc cagcagcaga tcgccgaggc gcgaaaacgc gacgacaagg ccgctgtggc
    17281 cgtctttgcc cgcgaactcg gcgaactccg ccaaaccgag atcgccaccg ctgacgcaca
    17341 catctaccag cccgccgtga tgaaactctc gcagcacagc agacaaaccg gcacgagcta
    17401 caaggacatc atcgtgcccg gctacgagcc gcacgatttc agcaccgaga aggccgcgca
    17461 aacgaggcat ttcctcgacg aggccgcgcg cctcagcatt ccaaccggac aacacgcggc
    17521 gtatgtcaaa catcgcgagg ccatgatgcg cctctggcct cgtgccgccg aactggacag
    17581 ccccgcgccg aacggccacc cgctgcggga gtttggccaa agtgaccgcg agttcgtcga
    17641 aaacgccaac agcgacgcca gcgtgcccca ggctctcgtt ttgatgaacg gccagatgaa
    17701 cacgcacatc ctcagtcgct ggtcgcaggt catgctcgcg atcaaacagg cgaaataccc
    17761 cgaagaccgc ctcgaagccg cctatctggc cctcttcagc cgcaaaccca ccagcgagga
    17821 aaaagcccga tggtctgcca ccgaggccaa tactgccgaa gacctcctcc acgctctcat
    17881 caacacgcgg cagttcattt ttgtgcagtg aggctgcggg gcgcataggc gaagtgaccg
    17941 cctcacatct ccgtttccac tgcatggcac ttcgtttcct ggttatcgcg cttctctcgg
    18001 cattcgtcat tccacattcg tcattcgcgg cgcagccgct gaacgtgctt ttcatcgccg
    18061 tggatgattt gcggcaggag atcggctgct atggcgcgga gcacataaag acgccgcatc
    18121 tcgatcagct cgcggcatcg gggatgctgt ttgagcgggc gtattgtcag gtcgcggtgt
    18181 gcaatccatc tcgcaacagc gtgctcagcg gcacgcgccc cgacacgaca ggcatttttg
    18241 acaaagtgaa caaagaacaa agtgtttgtc ccgaatggca cgggattaag gccggttttc
    18301 gacgaaaacg gctgatttca gccgtgcggc aggctggcat gagaagctct gaaactcttg
    18361 gcgctgcaag gttttgaaat gttcggaagc gcctgcttcc ttgattcaca gcgatgtttt
    18421 accggaagac cgccttaatc ccgtgccatt cgtgtctgcc cctttatcgc catcaaaaca
    18481 ggtattgccg gagaaatcgg tgtgtattac cattcgccac cgatgagcac tctctctctc
    18541 aagctgcctg attccctgct gctgcggctc gaccgggaaa gccggcagcg acgcatgaca
    18601 aagtccgcct tggtgcgggc ggcgttggag cgcgagctgg agcaacagcc gacggccaag
    18661 ggtgcctcgt gccatgatct ggctcgtgat ctggccggtt cgatcaaaaa gcggctgccg
    18721 aaggatctgg cgacgaatcc caagcacatg gagggatttg gccggtgagg cagacggtgc
    18781 tgctggacac aggaccgctg atctccttcc tcgccgccgg tctggggcac catgagtggg
    18841 tggtggagca gtggaaacgg ctgaaaccgc cgatgctcac ctgcgaggcc gttttgaccg
    18901 aggcggcctt tctgctcaaa cgcgagggag tggacaccga ttcgctcttt gccctgctgg
    18961 aacgcggggt gatccgcgtc gcgctggaga tcgaggatca agccgccgat ctgcgcacgc
    19021 tgatgcgccg ctaccgcaac cggccgatgt cactcgccga cgcctgccta gtgcgcctgt
    19081 ccgagctgca caaggacgcc gtcattttca ccctggatga tgatttccgc atctaccgcc
    19141 gccacggcaa caaggtgatc ccggtgctga tggcggagaa ttgaccggtt tgtctgagga
    19201 atgaccataa cgcgcagcag tctgaaaatg gcggcccttc tcttttcata gccagacgca
    19261 cgaagaagcc gggccatttg agcttgctcg gtgcaggcga aggaagacga ggggcccatg
    19321 acgcccaccc tgagcacccc ggtcgaagcc ttgctccgcg agcaggtcgc caagggtcac
    19381 tacccgagcc tggatcgggc cttggaagtc gcggtgaatg ccgttgatgg ccgtcgggca
    19441 tcccacgcgc tcgaatcgct gctgcacgag gcgcttccgc ctcccggcga ccgcgttccc
    19501 ctctagcaac agcgcgggac aacggcatga agcaggctcg tcacagaaga taatctggcc
    19561 tccagcccgc cgtttgaagc atttcgacaa ctcaggccga tgcttgtcat ctcagggcat
    19621 ggtggcttcc ttgcccttca tggacgaact gcaccgctgc tcacacaaca gcacgtttca
    19681 catgtcccgc tggacgaaaa agacgtggtg cgaagacgaa aatcacgtcc tccaggacgt
    19741 tttggacgag gtcgaagacg cttgaggcga cttcgaggac ggtttggacg aggttgaaga
    19801 cgttcgaaac gactttgagg acgttttgga cgccatcgaa gacgctttag acgacttcga
    19861 ggacgtcttt gacgaggtca aaaacggcct ttcagtattg cggcatgtgc cggtaaaaaa
    19921 tcataatcaa tgacttgcat aaaccgccgc cccccccctc ctcccctcgc cagcccagcc
    19981 agcattatcg gggcgctgat ccggactcta tatgggagag acctccgtgc ctgggctaag
    20041 tttccatccc ctcattatcg gggcgctgat ccggacccgg aaaagccacc ctggtcaccg
    20101 gcccagctgg tgaaagtttc catcccctca ttatcggggc gctgatccgg accgatggcg
    20161 atg
//
LOCUS       DAOMYJ010000080        20159 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Prosthecobacter sp. isolate AMR_MDS_5629
            NODE_931_length_20159_cov_22.280442, whole genome shotgun sequence.
ACCESSION   DAOMYJ010000080 DAOMYJ010000000
VERSION     DAOMYJ010000080.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867850
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prosthecobacter sp. (wastewater metagenome)
  ORGANISM  Prosthecobacter sp.
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Verrucomicrobiaceae; Prosthecobacter.
REFERENCE   1  (bases 1 to 20159)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 20159)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.30
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:34:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,742
            CDSs (total)                      :: 4,684
            Genes (coding)                    :: 4,676
            CDSs (with protein)               :: 4,676
            Genes (RNA)                       :: 58
            tRNAs                             :: 55
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20159
                     /organism="Prosthecobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_931_length_20159_cov_22.280442"
                     /isolate="AMR_MDS_5629"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1965333"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(134..2443)
                     /locus_tag="PLP58_10210"
     CDS             complement(134..2443)
                     /locus_tag="PLP58_10210"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HRJ08934.1"
                     /translation="MSKSKPSAAPPAASRSSALPLLLGIAGFIALVVWLLLPQADHIP
                     LPVSPSTPAAKITPYVMPEEKAAFAQYAGDASCAGCHAAEHAKWKSSNHGLAERLPDP
                     ALDLAAFDPAQTFQHGSQTTEVRKNGDFFEILSLGFDGRREPWKVERVIGHDPLRQFL
                     VHGTSGRLHAMEACFDPHKGDWFNVYGDEDRKPGEWGHWTGRGMVWNQMCASCHNTRL
                     RKNYDPATDSYRTSMAQLTVSCESCHGPMKAHDDWQRAHQGEKGDPHLVKWTRDQHIE
                     NCAGCHARRGEITGDFVPGESFWDHYLLTITDPSDIYHPDGQIRDENYEFASFLSSRM
                     HHAGVRCMDCHDMHSMRTILPGNQLCMRCHTPGGFPNSPPILPEVHSFHKAGSTGNQC
                     VNCHMSQTTYMQRDPRHDHGFVIPDPLLTKEHGVPNACNKCHQDQTVDWAIAAVDQWY
                     GPKMERHTRKRAFAMAEARQGKASGRDGLLALLQSDEIPHWKASATLTLDRWISEPRV
                     QEAVAAQLDHPHPLVRQSAARVLEPALADGRIRSRVEKLLEDTARSVRVTAAWALRET
                     LDPASAAGQDLQHMLRLNSDQPSGQMQLGQYHYARRDLPKAIQHMRTAVEWDPNSPPF
                     HHDLAMLYNAAGDTPKTIAKLRDAIRLAPRHAEYHYALGLALSETGDMPGTIAALRET
                     VRLDPAFARAWYNLGLALNGENQPEEAIAALIKGEQADPRDPGIPYARATIHARLGQK
                     EAAIHAARQALSRDPAYHDAQHLLQMLQR"
     gene            2567..3169
                     /locus_tag="PLP58_10215"
     CDS             2567..3169
                     /locus_tag="PLP58_10215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009962704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HRJ08935.1"
                     /translation="MTELRLTFCALVLAALCSCVSTSGPRGQTQYLEAFHPSAVPTSA
                     LYNQDLDSYWDGDGMSGPPSVTIDLSDQKAYFYKGGQLAGVSALSTGDEKHPTITGNF
                     KIIQKNQWHKSNLYGDFVDAYGNVVVANIDVTKDKPPPGTRFEGSKMHYFMRFHGGTG
                     MHEGFLPGYPASHGCVRMPGRMAAIFYNNVEVGTPVTVRP"
     gene            3185..3532
                     /locus_tag="PLP58_10220"
     CDS             3185..3532
                     /locus_tag="PLP58_10220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009962703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HRJ08936.1"
                     /translation="MNPQELPLEISVDDTSYLLTRPGAPPPRLIDCREQDEWQVCRLE
                     GAELVPLSAFGELAPQRFTDIHEHLIIYCHHGMRSQRAAAWLRQHGFRNAQSMRGGID
                     AWADLIDPAMPRY"
     gene            3538..4125
                     /locus_tag="PLP58_10225"
     CDS             3538..4125
                     /locus_tag="PLP58_10225"
                     /inference="COORDINATES: protein motif:HMM:NF020398.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1802 family protein"
                     /protein_id="HRJ08937.1"
                     /translation="MTDSPALPAPENATGFKEWALVCDLLVQGKQTVILRKGGIHEGR
                     GGFEWKHRGFFLFPTWFHTQAEKLRGESPQTSFPPEEERTSVDVDGFATLEQVWKVTD
                     WARVESISRFHAWNEEVVRERFAYDEESCLHIALVRAWRLPGRWTFPYSKSYGGCRSW
                     VSLPAEGLDLLPQASPAMSEAEWQQTAGELRALLD"
     gene            4222..5901
                     /locus_tag="PLP58_10230"
     CDS             4222..5901
                     /locus_tag="PLP58_10230"
                     /EC_number="2.7.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018970637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008741 - ribulokinase activity [Evidence
                     IEA];
                     GO_process: GO:0019569 - L-arabinose catabolic process to
                     xylulose 5-phosphate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulokinase"
                     /protein_id="HRJ08938.1"
                     /translation="MKQYALGIDYGTNSCRSLLIDLADGAEIGSSVFNYPSGEMGVLL
                     DPKDPHVARQNPQDYLDGLIAVTHGALKQAVGKVPGFSPSQVVGIGIDTTGSTPIPVN
                     REGTPLGLLPEFKDNLNAMVWLWKDHTAHAEAAEITALAHQMRPHIIAKCGGIYSSEW
                     FWSKILRLRRCDPQVFESAFSFVEHCDWLPAVLAGDTNPLTLKRGVCAAGHKAMFSTE
                     WGGLPDKEFLAQLDPSLGDLRDRLYSEAHTSDTRAGSLCAEWAAKIGLPEGTAIAAGA
                     FDAHMGAVGAGAKEGTLVKILGTSTCDLMITPNDKPLADIPGVCGIVNGSVLPGFFGI
                     EAGQSAVGDLFLWLVNHLVPEGYGATTGEKFAAMEKALSAQKPGASGLLALDWNNGNR
                     TVLVDARLTGLLLGQTLHTEAHEIYRAYIEATAFGALTIIRRVEEHGVKIDEIINTGG
                     LSIKNAALMQIYADVIGKPMKVSQSEQTCALGAAIFGAAAAGVDSIGALQARVTAVRE
                     KVYHPDPENAAVYQELYALYRTLHDAFGTREWSGNLHHVMKRLLEIRAGQG"
     gene            complement(5922..7727)
                     /locus_tag="PLP58_10235"
     CDS             complement(5922..7727)
                     /locus_tag="PLP58_10235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009958937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arylsulfatase"
                     /protein_id="HRJ08939.1"
                     /translation="MRAFFLLALLLSFTGPVSAAPPNLVVFLADDAGWGDYGASGNKQ
                     LATPNIDSLAHGGVTLDRFYVCPVCAPTRAEFLTGRYHPRGGVRGVSTGQERLDFGEK
                     TLADAFKAAGYATGAFGKWHNGSQWPYHPMARGFDEYFGHTSGHWGEYFDAPLEENGR
                     MIRTRGYIVDVCTDRALDFIGRNSSKPFFCYIPFTTPHSPWAAPEKDWQRFKDKPLTQ
                     FATLAEQEVADETRCAYAMLENQDWNVGRVLARLKEAGVEDNTIVVYFSDNGPNSNRW
                     TGGMKGKKGQTDEGGVRSVCFIRWPSRLPAGHTVTQICGAIDLLPTLTALAGVARVGD
                     KPLDGRDLSPLLAGQTVEWSERTIFNCWNQNISARTQTHRLDNTGKLFDMIADPGQTT
                     PVNDKQPDLAARLAAEVKAWRKEMFGTDQAPASPAKAGKGKGKGGANAVDPRAFPVGW
                     REFPVTMLPARDGEPRGGVQRSSGAPNCSYFVNWTSVNDSMVWLIDVNTAGSYDVSVD
                     YTCPLTDAGSVIELEFKGSRLSGKVQPGWDPPLYTNQDTLPRPHGESTMKEFRTLSLG
                     KMRLESGQGTLTLRALEIPGKSVMDVRRVTLTLLE"
     gene            7862..8350
                     /locus_tag="PLP58_10240"
     CDS             7862..8350
                     /locus_tag="PLP58_10240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009960884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MogA/MoaB family molybdenum cofactor
                     biosynthesis protein"
                     /protein_id="HRJ08940.1"
                     /translation="MTSTILTGIITVSDRASQGHYEDLGGPALKEAALGHGWEVAAEV
                     IVPDDLERIQQAVLALEAQGCGLILTTGGTGIAERDVTPEALRGIMRVEIPGFGEVMR
                     MRSLASTPNAILSRCLAAVVGRSLVIALPGKPAGAVECLGYARDAIPHSVKLARGVPT
                     SC"
     gene            complement(8428..9657)
                     /locus_tag="PLP58_10245"
     CDS             complement(8428..9657)
                     /locus_tag="PLP58_10245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ08941.1"
                     /translation="MKLNPILTAILLASAVVPAIAKWSRLPSPVSRPTSISTVLNGTS
                     IQESSRIGQANHLILADPLKPVALNAGKSSATLKLSRQSVVHSVSFVSEGLEGRVELA
                     LSADGKAWSQQQTSAFTPLDRLVKLSAGAAQGRFLRLDFDLVRGGSLRSFQVIGTDTD
                     ANYTVKQNADGTGAPVNFAAGIGGGRLLYINPELYGARGDAARLGRLEFPESDEKYRT
                     AVYDFGQVRTLNEFGSVHSPRPVRLSVFAFDELPEKEDWRGRLAFDPSVFDTAEPVAT
                     IEDRKGTGVVRAKPKSAVKARYIAMRWEPDFNPPGFTVDDISINGFGDVSYRNGDTEV
                     DTTGDGEGDTESTVKDGEGTESFKTEDGGGEDGGSGPPMMSVAPGAISAQGAGIAGAP
                     GEEEKAKGTDPDPPTPQ"
     gene            9831..10469
                     /locus_tag="PLP58_10250"
     CDS             9831..10469
                     /locus_tag="PLP58_10250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ08942.1"
                     /translation="MMNCPHCSTTVVAGTASCPACGFSTPAIRALLGGDWVRLERITD
                     RSQRLSLRDTRHLEVCLDDFERAFPQCFLALYLGPLPDNLTPCDLGFWMLNHGAFQSL
                     QIMKQNEFGIALVLDTLGQRAGITAGYALERHLPPDFLHLLLISVGPRLRKGALASAV
                     ESVVAQTARRLKTSARPAPRPAPGQAVVLPGSGLSCFEPLRQRSDSATTLKS"
     gene            10655..11533
                     /locus_tag="PLP58_10255"
     CDS             10655..11533
                     /locus_tag="PLP58_10255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ08943.1"
                     /translation="MPSPEKPPTRLFMEDGALQSDDLNLFLQGGPEQARRPAPDPVSS
                     PLRELSPARIAGLRSLPRDTWLMDPLALLPAPQSQSLHRLLQYHAANATSRLHLLVIE
                     QDQFLPDQANPGLFAGGALSGNNVALIVCPLGETWRSRFYLGSSLREGVPAHLAAEMI
                     RDCVRDSQLVLEPEQQVQRLAVRASTWLFRLENFLKPAPAQSLRNPLREIAPAINSSA
                     EAPGNLSVLWLPAFGLAFLLSLMLWRHSRRATFPDERIWLLEEPEIVPRLGGAFCGGG
                     GAVLHHGKTGGPRSKA"
     gene            11601..13265
                     /locus_tag="PLP58_10260"
     CDS             11601..13265
                     /locus_tag="PLP58_10260"
                     /inference="COORDINATES: protein motif:HMM:NF013662.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-ubiquinone oxidoreductase-F iron-sulfur
                     binding region domain-containing protein"
                     /protein_id="HRJ08944.1"
                     /translation="MSKNLSALSFRKGIEKNVFERMVEAAEKHGTAEKNDLVHQMGQD
                     HLFGDAITLGAVSFYDFLKKENEGKKVFVCNGSACLCAGTQDKLHHTLETHFKKDEIG
                     HICCLGRCHEGAALQYQGKNYSGQSDAALGDLFRTGQGDSEDRYAVVSHLQPAILTAE
                     FKGIEDYYAPFKKLITEGNRDALAAELKDSGLRGRGGAGFPLSIKWAGCRAAEGAVKY
                     VVCNADEGDPGAYIDKYLMEKQPHSVLLGMMVAGWYAGAETGVLYIRAEYPDSVTIVN
                     QAITDLHEAGLLGQNILGTGFNFALKSIKGAGAYICGEETALLASIEGQRPEVRTRPP
                     FPVNEGLYRKPTIVNNVETFANLRAILTVGGKGYAQIGTPQSTGPKLLSLDSHFVKPG
                     IYEVAMGTPLQTVIDLAGGFKTKIKAIQIGGPLGGIVPMSHIAQLTVDFESFKKAGFL
                     LGHAGVVSIPETMPMIEYIQHLFQFTADESCGKCFPCRLGSTRGKELIAKARGDTAFK
                     IDRELLSDLLDTMEQTSLCALGGGVPLPIKNALEHFDGELKGFFRP"
     gene            complement(13285..14904)
                     /locus_tag="PLP58_10265"
     CDS             complement(13285..14904)
                     /locus_tag="PLP58_10265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007414572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate permease"
                     /protein_id="HRJ08945.1"
                     /translation="MTWSQNYDPLGSLILSSLVAALPVVVLLGLLAFWHVRAQLAALA
                     GLFAALLIAVFVYHMPAQLAGMAAVNGAAFGLFPIGWIVLNAMFVYNLSVETGQFAVL
                     QKQIAGVSSDRRVQALLIAFSFGAFIEGAAGFGAPVAITGALMIGLGFKPLEAAKLAL
                     IGNTAPVAFGSLGTPLTTLADITGLDLMQLSAMVGRQLPLFSLIVPFWLVAAQVGWRG
                     MLGVWPACLVTGLSFGVMQFVMSNYHGPWLVDIVSAVVAMAALVGFMKVWRPKSADAH
                     ANEEAAGGALGAAWKAWLPWIFLTAFVFAWGMPAVKNGLNAAFNQEIEVPLLHKAVER
                     VPPVVVHTEFEKAVFKFNALSATGTAAVLAGVLAGFCLGLGPVRIAKLYAATIWKLRV
                     PLLTISLMLALGYVTRYSGTDTTMGLMMAGTGVLFPFFSPIIGWLGVALTGSDTSSNV
                     LFGNLQQVTANQLDLSPVHMAASNSSGGVMGKMIDAQSIVVAAVATGGHPDSPDAGTV
                     LRSVFWHSVALAILMGLLVMAQAYWLPWMIE"
     gene            complement(14901..16142)
                     /locus_tag="PLP58_10270"
     CDS             complement(14901..16142)
                     /locus_tag="PLP58_10270"
                     /inference="COORDINATES: protein motif:HMM:NF014774.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heterodisulfide reductase-related iron-sulfur
                     binding cluster"
                     /protein_id="HRJ08946.1"
                     /translation="MLHSIPTDKLGPLGEPMAKAVSACVHCGFCLSACPTYRELGQEM
                     DTPRGRIVLMKEVLEGKMSFEAAQPHVDRCLGCLACEPACPSGVPYRDLISPFRALAA
                     GKMKRGWLERLRRFLAAQTVPFPGRFRLAAWLGKHVPAPSMMKPMLELIPDELPPKQT
                     WEECYPASGEKRARVALLLGCAQQVLDPDINTATIEVLTRNGVEVVIPRSQGCCGGLA
                     WHTGDLEAARSFARQNLGAFPEDVDAILTNAAGCGSAMHEYHLVLRGTPDETRAEAFR
                     HRVMDVSVFLARLGLRESLRETKLKAAYHDACHLSNAQNVRKQPRDLLKAIPGLQLLE
                     ISDAHLCCGSAGSYNMDQPDIASSLGEQKAKNIIATGAEAVVTGNIGCMTQVNLHLKK
                     LGSPIQVKHTMQVIRDAMLPS"
     gene            16277..16537
                     /locus_tag="PLP58_10275"
     CDS             16277..16537
                     /locus_tag="PLP58_10275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ08947.1"
                     /translation="MKLTLSRVLLLLVATVLFGVFTPNHQSLFAAGPPNKEPGKVPPD
                     AAKGRQLAEFIKQAKARGLKGSELARAIAEERKRLGMPPEDD"
     gene            complement(16564..17511)
                     /locus_tag="PLP58_10280"
     CDS             complement(16564..17511)
                     /locus_tag="PLP58_10280"
                     /inference="COORDINATES: protein motif:HMM:NF013714.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HRJ08948.1"
                     /translation="MIAPSTLTELRDAVLSAPRVIAVGAETKPRLSAVEAVKISTRQL
                     SGITEYEPSEFTFTALAGTPVREIIAALDAKGQYLPFDPMLVEAGSTIGGVVASGLSG
                     PGRFRYGGVRDFILGVRFVDGMGRLLKLGGKVVKNAAGFDVPKFLVGSLGRFGAIGEV
                     TFKVFPKPESTLTLQMPFSAEKMGKLANSRFEIDALDVPPGGETMLVRLAGPAKALEA
                     LAKEIGGKIIDNAVWREIRETPWSFKTDITPDQINDFKTAHVSCGGNVALSNTPPPRG
                     MTLRGDSPLWIGGRPRFAIEDTVKQALDPQNRFPTLDNN"
     gene            complement(17508..18950)
                     /locus_tag="PLP58_10285"
     CDS             complement(17508..18950)
                     /locus_tag="PLP58_10285"
                     /inference="COORDINATES: protein motif:HMM:NF014910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-linked oxidase C-terminal domain-containing
                     protein"
                     /protein_id="HRJ08949.1"
                     /translation="MSTALDRLAAVLPPERLLMRPVELAAYESDGLTAFRTRPLAVVV
                     PETTDEVIAIVRLCHELKLPFVARGSGTSLSGGSLPIAEGIVIALNRLNRILKLDPEQ
                     RIAVVEPGVVNTQVTTAAAPFGLHYAPDPSSQSICTIGGNVAFNSGGAHCLKYGMTAN
                     HMLGIKAVLATGEIVEWGGGSRERLGPDWCGLFTGNEGLFGIALEITLQLLPKAECFH
                     TVLAGYRTLEQAGDAVSAVIASGLLPGAIEIMDALALEAAVAAVHAEYPPGCEAVLIV
                     ELEGPREVVAIERERLDAILAASAPVEMRPARDAEERLAIWKGRKSAFSAVGRLSPDF
                     IVQDGVVPRRRLGEALRRIAEMSQETGLRVANVFHAGDGNLHPLILFDGREEGALHRA
                     EDLAGRILKMCVEMGGSITGEHGIGVEKRDFLPDMFTADEIDCMQRLRAAFDPLGIAN
                     PGKMFPGSEAPSLSHVGLHPLEKAGVISRE"
     gene            complement(18961..20070)
                     /locus_tag="PLP58_10290"
     CDS             complement(18961..20070)
                     /locus_tag="PLP58_10290"
                     /inference="COORDINATES: protein motif:HMM:NF012488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--glyoxylate aminotransferase family
                     protein"
                     /protein_id="HRJ08950.1"
                     /translation="MNTQFPAERILLGPGPSPVPSRVLRALGAPTLGHLDPQYVAIMD
                     EVCEMLRQVFRTKNSLTFPVSGTGMAGMECIATNLIEPGDEVIVCVNGVFGGRMKDVM
                     ERCGATVHAVEAAWGDVIQFDQIAAALDQHPKSKLVGIVHAETSTGALQPLEGLAELV
                     HSKGALLVVDAVTSLGGHELKVDDWGIDAIYSGTQKCLSCPPGLAPVSFGDRALAIMD
                     SRKTKVQSWYLDVSMLRKYYTSGGGGRVYHHTAPINMTYALREALAIVLEEGLEARIT
                     RHAQMHQRLRAGLEAMGISYVPKHSLHTLNCIHIPAGADDAGTRKRLLEEYGIEIGAG
                     LGVMAGKAWRIGLMGHGATVRNVDLVLTALQNVLK"
BASE COUNT         3655 a         6516 c         6352 g         3636 t
ORIGIN      
        1 ggcggcgcgc cggagccact ccgcgcgcgc caccactttt tgcgggtctt ggagtgttgg
       61 agcgatgacg ctgtctttcc aattctcccg cctccatgac tccaatgggg catggcttca
      121 caaaggcggc ggctcagcgc tggagcatct ggaggaggtg ctgcgcgtcg tggtatgcgg
      181 ggtcgcggct gagggcctgg cgggcggcgt ggatggccgc ctctttctga ccgaggcgtg
      241 cgtggatggt ggcgcgggcg taagggatgc cggggtcgcg cgggtcggcc tgctcgcctt
      301 tgatgagggc ggcgatggct tcctcgggct ggttttcgcc attgagggcg aggccgagat
      361 tgtaccaggc gcgggcgaag gcggggtcaa gccgcacggt ctcgcggagg gctgcgatgg
      421 tgccgggcat gtccccggtc tcgctgaggg cgagtccgag ggcgtagtgg tactcggcat
      481 ggcgcggggc gaggcggatg gcatcgcgca atttggcgat ggttttgggg gtgtcgccgg
      541 cggcattgta gagcatggcg agatcgtgat ggaagggggg ggagtttggg tcccactcga
      601 cggcggtgcg catgtgctgg atggctttgg gcaaatcccg ccgggcgtaa tgatactggc
      661 cgagctgcat ctggccgctg ggctggtcgg agttcagccg gagcatgtgc tggaggtcct
      721 gcccggcggc ggaggcgggg tcgagcgtct cgcgcaaggc ccaggcggcg gtgacgcgga
      781 cgctgcgggc ggtgtcctcc aggagtttct ccacccggct gcggatgcgg ccatccgcga
      841 gggcgggctc aagcacgcgc gcggcggact ggcgcacgag cggatgcggg tggtcgagtt
      901 gtgcggccac agcctcctgg acgcgcggct cgctgatcca gcgatccagg gtgagtgttg
      961 cgctggcttt ccagtggggg atttcatcgc tctggagcag ggccagcagt ccatcccgtc
     1021 cgctggcttt gccctggcgg gcctcggcca tggcgaaggc gcgcttgcgg gtgtggcgct
     1081 ccattttcgg gccgtaccat tggtccacgg cggcgatggc ccagtccacg gtttgatcct
     1141 ggtggcattt gttgcaggcg ttggggacgc cgtgctcctt ggtgagcaga gggtcgggga
     1201 tgacgaagcc gtggtcatgg cggggatcac gctgcatgta ggtggtctgg gacatgtggc
     1261 agttgacgca ttggttgccg gtgctgccgg ctttgtgaaa gctgtggact tcgggcagga
     1321 tgggcgggga attggggaag ccgccggggg tgtggcacct catgcagagc tggttgccgg
     1381 ggaggatggt ccgcatgctg tgcatgtcgt ggcagtccat gcagcggacg ccggcgtggt
     1441 gcatgcggct gctgaggaag gaggcgaact cgtagttctc atcgcggatc tggccgtcgg
     1501 ggtggtagat gtcggagggg tcggtgatgg tgagcaggta gtggtcccag aaggattcgc
     1561 cggggacgaa gtcgccggtg atctcgccgc gcctggcgtg gcagccggcg cagttttcga
     1621 tgtgctggtc gcgggtccat ttgacgagat gtgggtcgcc tttttccccc tgatgggcgc
     1681 gctgccagtc gtcgtgcgct ttcatggggc cgtggcagga ctcgcaactg acggtgagct
     1741 gggccatgct ggtgcggtag ctgtcggtgg cgggatcgta gtttttgcgc aggcgtgtgt
     1801 tgtggcagga ggcgcacatt tgattccaga ccatgccgcg cccggtccag tgcccccact
     1861 cgccgggttt ccgatcctcg tctccataga cattgaacca gtcgcccttg tgcggatcaa
     1921 agcaggcctc catggcgtgc aggcggccgc tggtgccgtg gacgaggaac tggcggaggg
     1981 gatcgtgccc gatgacgcgc tcgactttcc agggctcgcg gcgcccgtcg aagccgaggg
     2041 agaggatttc aaaaaagtcg ccgtttttcc gcacctcggt ggtctggctg ccgtgctgga
     2101 aggtctgcgc ggggtcgaag gcggcgaggt cgagcgcggg gtcgggcagg cgctcggcga
     2161 ggccgtggtt ggagcttttc cacttcgcgt gctcggcggc gtggcacccg gcgcaggagg
     2221 cgtccccggc gtattgggcg aaggcggcct tttcctccgg catgacatag ggggtgatct
     2281 tcgccgccgg cgtggatggg ctgaccggaa gcgggatgtg gtcggcctgg ggcagaagca
     2341 gccacaccac cagggcgatg aagcctgcga tgccgagcag caggggcagc gcgctgctgc
     2401 ggctggcggc tggcggggcg gctgacggct tggatttgct cattccaggc tattacgcgg
     2461 gagggcggcg ggtgtccaaa gtttcgtgga accattggac aggcgggagg cgttgcttca
     2521 ttctggcggg ccttttcccg ccccctgaaa aacccccgct ttttcgatga ccgaactccg
     2581 cctgactttc tgcgcgctcg tgctggccgc gctgtgctcg tgtgtgtcca cttccggccc
     2641 gcgcggccag acgcagtatc tggaggcttt ccacccgtcc gccgtgccga cctcggcttt
     2701 gtacaaccag gacctggact cctactggga tggcgacggg atgagcgggc cgccgagtgt
     2761 gacgatagac ctgagcgacc agaaggccta cttttacaaa ggcggccagc tcgcgggcgt
     2821 gtcggcgctg tccacagggg atgagaagca cccgaccatc acggggaact tcaaaatcat
     2881 ccagaagaac cagtggcaca aatccaacct gtacggggac tttgtggacg cctacggcaa
     2941 cgtggtggtg gcgaacatag acgtgacgaa ggacaagccg ccgcccggca cgcgcttcga
     3001 ggggtcgaag atgcactact tcatgcgctt ccacggcggc acgggcatgc acgagggctt
     3061 cctgcccggc tacccggcct cccacggctg cgtgcgcatg ccgggcagga tggcggccat
     3121 tttttacaac aatgtggagg tgggcacgcc cgtgacggtg cggccctgat ccgggagcga
     3181 ggtgatgaat ccgcaggaac tgccgctcga aatcagcgtg gatgacacga gctatctgct
     3241 gacgcggccc ggcgcgccgc cgccgcgcct catagactgc cgcgagcagg acgagtggca
     3301 ggtgtgcagg ctggagggcg ccgagctggt gccgctctcc gccttcgggg agctggcgcc
     3361 gcaaaggttc accgacatcc atgagcacct catcatctac tgccaccacg gcatgaggag
     3421 ccagcgcgcg gcggcttggc tgcgccagca cggcttccgc aacgcgcagt ccatgcgcgg
     3481 cggcattgac gcctgggccg acctcattga ccccgccatg ccccgctact gacgcccatg
     3541 acagactccc ctgccctgcc cgccccggaa aacgccaccg gcttcaaaga atgggcgctc
     3601 gtctgcgacc tgctcgtcca gggaaaacag acggtcatcc tgcgcaaagg cggcattcac
     3661 gaggggcgcg gcggctttga atggaagcac cggggctttt tccttttccc cacctggttc
     3721 cacacccagg cggagaagct gcgcggcgag tctccgcaga cgagcttccc gccggaggag
     3781 gagcggacca gcgtggatgt ggacggcttt gccacgctgg agcaggtgtg gaaagtcacc
     3841 gactgggcca gggtggagtc catctcccgt ttccatgcct ggaacgagga ggtggtgcgc
     3901 gagcgcttcg cctatgacga ggaaagctgc ctgcacatcg ccctcgtgcg cgcctggcgg
     3961 ctgccggggc gctggacttt tccatattcc aaaagctacg gcggctgccg ctcctgggtg
     4021 agcctgcctg cggaaggact cgatctcctg ccccaagcca gccccgccat gtccgaagcc
     4081 gaatggcagc agaccgccgg cgaactgcgc gcgctgctgg actgaggcgg acggcggcgc
     4141 cgggcgcgcg ggtgtctttt cgtggcgcgc tgatttttcg ctttcccaag ctggcgtccg
     4201 ctccctacct tggcggaccg catgaaacag tacgcactcg gcatagacta cggcaccaac
     4261 tcctgccgct ccctcctcat tgacctcgcc gacggcgcgg agatcggctc cagcgttttc
     4321 aattatccct ccggcgagat gggcgtgctg ctcgacccga aggatcctca tgtggcccgc
     4381 cagaatccgc aagactacct tgacggtctc atcgccgtga cacacggcgc tctcaaacag
     4441 gccgttggca aagtgcccgg cttctcgcca tctcaagtcg tcggcatcgg catagacacc
     4501 accggcagca cgcccatccc ggtgaaccgc gagggcactc cgcttggcct gctgcctgaa
     4561 ttcaaagaca acctcaatgc catggtctgg ctgtggaagg accacaccgc gcatgccgag
     4621 gctgcggaga tcaccgcgct cgcccatcag atgcggccgc acatcatcgc caaatgcggc
     4681 ggcatctact ccagcgagtg gttttggagc aaaatcctgc gcctgcgccg ctgcgacccg
     4741 caagtgttcg agtccgcctt cagctttgtg gagcattgcg actggctgcc cgccgtgctg
     4801 gcgggcgaca ccaaccctct caccctcaag cgcggcgtct gcgccgccgg gcacaaggcc
     4861 atgttcagca ccgaatgggg cggcctgccg gacaaggaat tcctcgcaca gctcgacccc
     4921 tcgctgggcg atctgcgcga ccgtctttac agcgaggcgc acacctctga cacacgcgcc
     4981 gggagtcttt gcgccgagtg ggccgccaag atcggcctgc ccgaaggcac cgccatcgcc
     5041 gccggcgcct tcgacgcgca catgggcgcg gtgggcgccg gcgccaaaga gggcacactg
     5101 gtgaaaatcc tcggcaccag cacctgcgac ctcatgatca cacccaacga caagcccctg
     5161 gccgacatcc ccggcgtctg cggcatcgtc aatggcagcg tgctcccggg tttcttcggc
     5221 atcgaggccg gccagagcgc ggtgggcgat cttttcctgt ggctggtgaa ccacctcgtg
     5281 cccgagggct acggcgccac caccggcgaa aaattcgccg ccatggaaaa agccctctcc
     5341 gcccaaaaac ccggcgccag cggcctgctc gcgctcgact ggaacaatgg caacagaacc
     5401 gtgctcgtgg acgcccgcct caccggcctg ctgctcggcc agaccctgca caccgaagcc
     5461 catgagattt accgcgccta catcgaggcc accgccttcg gcgcgctcac catcatccgg
     5521 cgcgtcgagg agcacggcgt caaaattgac gagatcatca acaccggcgg cctctccatc
     5581 aaaaacgccg ccctcatgca gatctacgcc gatgtcatcg gcaagcccat gaaggtcagc
     5641 caaagcgagc agacctgcgc cctcggcgcc gccatcttcg gcgcggcagc cgccggcgtg
     5701 gacagcatcg gcgccctcca agcccgcgtc accgccgtgc gtgaaaaggt gtatcacccc
     5761 gatcccgaaa acgcggctgt ttatcaagag ttgtatgcat tgtatcgaac gctgcacgac
     5821 gccttcggca cacgcgaatg gagcggcaac ctccaccacg tcatgaagcg cctgctggag
     5881 atccgcgcgg ggcagggttg agcgctctcc gatcccccgc tttactcaag cagcgtcagc
     5941 gtcacgcggc gcacatccat gacggatttg cctgggattt ccagggcgcg gagggtcagt
     6001 gttccctggc cggactccag gcgcattttg ccgaggctca gcgtgcggaa ctccttcatc
     6061 gtgctttcgc cgtgcgggcg cggcagggtg tcctggttgg tgtaaagcgg cggatcccag
     6121 cccggctgca ccttgccgga gaggcggctg cctttaaact ccagttcaat gacggaaccg
     6181 gcgtcggtca gagggcaggt gtaatccacg ctcacgtcat aagatcccgc ggtgttcaca
     6241 tcaatgagcc agaccatgct gtcattgacg ctggtccagt tgacgaaata tgagcagttc
     6301 ggagcgccac tgctgcgctg gacgccaccg cgcggctcgc cgtcacgggc gggcagcatg
     6361 gtgacgggaa actcccgcca gccgacaggg aacgcgcgtg gatcaaccgc gttggcaccg
     6421 cctttgcctt tgcccttgcc tgccttggct ggagatgccg gagcctggtc cgtgccgaac
     6481 atttccttgc gccacgcttt gacctcggct gctaggcggg cagccagatc aggctgcttg
     6541 tcattgacgg gcgtcgtttg cccgggatcg gcgatcatgt cgaaaagttt gcccgtgttg
     6601 tcgagccggt gcgtctgcgt acgggcgctt atgttttgat tccagcagtt gaagatcgtc
     6661 cgctcagacc actccaccgt ttgcccggcc agcagaggag agagatcgcg cccgtcgagg
     6721 ggcttgtcac cgacgcgggc gacaccggcc agcgcggtga gggtgggcag caggtcaatg
     6781 gcgccgcaaa tttgagtgac tgtgtgtccg gccggcagtc tcgacggcca gcggatgaag
     6841 cagacggagc gcacgccgcc ctcgtccgtc tggcctttct ttcccttcat gcctccggtc
     6901 cagcggttgc tgttcgggcc gttgtcggag aaataaacga cgatggtgtt gtcctcgaca
     6961 ccggcctcct tgagacgggc gagcacgcgg cccacattcc agtcctggtt ctccagcatg
     7021 gcgtaggcgc agcgtgtctc atccgccact tcctgctccg cgagcgtggc gaactgggtg
     7081 aggggcttgt ctttgaagcg ctgccagtcc ttttccgggg cggcccaggg cgagtggggc
     7141 gtggtgaaag ggatgtagca gaagaagggc ttcgaggagt tccgcccgat gaaatcgagg
     7201 gcgcggtcgg tgcagacatc cacgatgtaa ccgcgcgtgc ggatcatgcg cccgttctcc
     7261 tccagagggg cgtcaaagta ctcgccccag tggccggagg tgtggccgaa gtattcatca
     7321 aatccgcgcg ccatcggatg atagggccac tgactgccat tgtgccattt gccaaaggct
     7381 ccggtggcgt aacccgccgc tttgaaggcg tcggccagcg ttttctcacc aaagtccagc
     7441 cgctcctggc cggtcgagac gccgcgcaca cctccgcgcg gatgatagcg cccggtcaga
     7501 aactcggcgc gtgtcggcgc gcagaccgga cacacataaa accggtccag cgtcacgccg
     7561 ccatgcgcca gggagtcaat gttcggcgtg gcgagctgct tgttcccgga ggcgccatag
     7621 tcgccccagc cagcgtcatc ggcgagaaag accaccaaat tcggcggagc cgcggaaacc
     7681 ggaccggtga aagacaagag gagggcgaga aggaaaaaag cgcgcatgac ggctgatggg
     7741 aacagcgcaa cacggcgggg tctttcgccg attgccgagc gcgcggagcg atgccgccag
     7801 caaagcactt gagagcgggc ggcgcggcgt tgatagagcc ggaggcaatc cctccgactc
     7861 catgacctcc accatcctca ccggcatcat cactgtttcc gaccgcgcca gccagggtca
     7921 ctatgaggac ctcggcggcc cggctttgaa ggaggcggcg ctgggccacg gctgggaggt
     7981 ggcggcggag gtcatcgtgc cggacgatct ggagcgcatc cagcaggcgg tgctggctct
     8041 ggaggcgcag ggttgcggac tcattctcac cacgggcggc acaggcatcg cggagcggga
     8101 tgtcacgccg gaggcgctgc gcggcatcat gcgcgtggag attcccggtt ttggcgaggt
     8161 catgcgcatg cgctccctgg cctcgacacc gaacgccatt ctcagccgct gtctggccgc
     8221 tgtggtgggc cgcagcctcg tcatcgcact gccgggcaag cctgcgggcg cggtggagtg
     8281 cctcggttat gcgagagacg ccatcccgca ctccgtgaag ctggcgcgcg gagtgccgac
     8341 gagctgctga cgatgcgcgg cctcacaacg aaaacgcgcg gaccgtggag gcccgcgcgt
     8401 tgaaaaaatt ccggctgatc accgctctca ctggggagtt ggcggatcag gatcggtgcc
     8461 tttggctttc tcctcctcgc cgggcgctcc agcaatgccc gcgccctgcg cgctaatggc
     8521 acctggtgcc acagacatca tgggaggccc ggaaccgccg tcctctccgc caccatcctc
     8581 ggttttgaag gattcggtcc cctccccgtc cttgacggtg gactcggtgt cgccctctcc
     8641 gtcgccagtg gtatccacct cggtgtcacc attgcggtag ctgacatcgc caaatccgtt
     8701 gatcgaaatg tcatccacag tgaagccggg cgggttgaag tcaggctccc agcgcatggc
     8761 aatgtagcgc gccttcactg cgctcttcgg cttggcacgc acgacgcccg tgcccttccg
     8821 gtcctcaatg gtggccaccg gctcggcggt gtcgaacacg gaggggtcaa acgcgaggcg
     8881 tccacgccag tcttctttct ccggcagttc gtcgaaagca aacacgctca agcgcacagg
     8941 acggggcgaa tgcacggagc cgaattcatt gagcgtccgc acctggccaa aatcataaac
     9001 cgctgtgcgg tatttctcgt cggattcagg gaattccagt ctgccaagcc gggccgcgtc
     9061 gcctcgcgcg ccatagagtt caggattgat gtagagaagc cttccgccac cgatgcctgc
     9121 ggcgaagttg accggcgcac cggtgccgtc agcgttttgc ttgaccgtgt agttggcgtc
     9181 cgtgtcggtg ccgatgacct ggaaactgcg caggctgccg ccgcgaacga gatcgaagtc
     9241 gagccgaaga aagcgcccct gagcggctcc ggcactgagt ttgaccagcc tgtctagcgg
     9301 agtgaaggcg ctggtctgct gctgcgacca ggctttgcca tccgcgctga gagccagttc
     9361 caccctgccc tccaatccct cggagacaaa cgagacgctg tgaacgacgg actggcggga
     9421 gagcttcagc gtggcactgc tcttgccggc attgagggcc accggcttga gcggatcggc
     9481 aaggatgaga tggttcgcct ggccgatgcg ggaggactct tggatggagg tgccgttgag
     9541 caccgtgctg atcgaggtcg ggcggctgac cggactgggc agcctgctcc atttggcaat
     9601 ggccggaacg acggccgagg caagcaggat ggcggtaagg atggggttta atttcatgat
     9661 gccatgggaa gtggccgcct tccaaagatg ggggggaatg cggccggggt tttcgatgcg
     9721 gagtttattg tgcatggcag gcggctgtaa accccttttt ctcgacataa agccgctccc
     9781 tattgaccgc ttgcccgaaa ctcccggacg gggttaatta ccccccaagg atgatgaact
     9841 gcccgcattg ctctacgacg gtggtggctg gaaccgccag ttgcccggcg tgcggctttt
     9901 ccaccccggc catccgcgcg ctgctgggcg gggactgggt gcgtctggag cgcatcaccg
     9961 accgctctca gaggctgtcc ctgcgcgaca cccggcatct ggaggtctgc ctcgacgatt
    10021 tcgagcgcgc tttcccgcaa tgcttcctcg ccctctatct cggccccctg ccggacaatc
    10081 tgaccccttg cgacctggga ttctggatgc tgaatcacgg ggcttttcag agcctgcaaa
    10141 tcatgaaaca aaatgagttt ggcatcgccc tggttctgga cactcttggc cagagggccg
    10201 gcatcactgc gggttatgcc ctggagaggc atctgcctcc cgactttctc catctgctcc
    10261 taatttccgt tggaccaagg ctgcgcaagg gcgctctggc atcagcggtg gagtcggtgg
    10321 tcgctcaaac ggccaggcgt ctgaaaacct ccgcgcgtcc agccccgcgt ccagcccctg
    10381 gacaagccgt ggtcctgcca ggatccgggc tttcttgctt cgagccgctg cgccagcgct
    10441 ccgattctgc aaccaccctg aaatcatgaa tgcgcgctgg aaattccctg ccgtcatggc
    10501 cgccatgctg ccactgctga tgacgcacgc gcggagtcat gcatcggcgg acgaacccgc
    10561 tctgccagtc tgggatcccc ggtcaaacgc acccggctcc acaggcggct cgctggtccc
    10621 atttctgccg cctccaagtg aggctcccgc gggaatgcca agcccggaaa agcctccgac
    10681 ccgtcttttc atggaggacg gcgcgctcca gtccgatgac ctgaacctct tcctgcaagg
    10741 cggcccggaa caggcgcggc gccccgcacc agacccggtc tcatcacccc tgcgggagct
    10801 ttcacccgcg cgcatcgccg gattgcgctc cctgccgcgc gacacctggc tcatggaccc
    10861 gctcgcactc ctgccggcgc cgcaaagcca gagccttcac cgtcttcttc aataccacgc
    10921 cgctaatgcc accagccgac tccatctgct ggtcatcgag caagatcaat tccttcccga
    10981 ccaggcaaac cccggccttt ttgccggcgg cgcgttgtct ggaaacaacg ttgcactgat
    11041 cgtctgcccg ctgggcgaga cttggcgctc gcggttttat ctcggcagca gcctcagaga
    11101 gggcgtgccc gcgcatctgg cggctgaaat gatccgcgac tgtgtcagag actcgcaact
    11161 cgtcctcgaa cccgagcagc aggtgcagcg cctcgcggtg cgcgcctcca cctggttgtt
    11221 ccggctcgaa aacttcctca aaccagcgcc ggctcaaagc ctccgcaacc cgctgcgtga
    11281 aatcgctccc gccatcaact ccagcgcgga agcccccggc aacctgtccg tcctctggct
    11341 gccggcattc ggcttggcat tcctgctctc cctcatgctc tggagacact cgcgccgcgc
    11401 caccttcccc gatgaaagga tctggctgct ggaggagccg gagatcgtgc cgcggcttgg
    11461 cggcgcgttt tgcggcggcg ggggagccgt gcttcatcat gggaaaaccg gcggcccgcg
    11521 ctcaaaagcg tgagtttttt ctggccttgg ccgcgtcttc atgagtatag catttcagcc
    11581 acggctgaac ctctccaacc atgtccaaaa acctcagcgc cctatccttc cgcaaaggca
    11641 tcgaaaagaa cgtcttcgaa cgcatggtcg aagccgccga aaagcatggc acggccgaaa
    11701 agaacgatct cgtccaccag atgggccagg atcacctctt tggtgatgcc atcacgctcg
    11761 gcgctgtgag cttctacgac ttcctcaaaa aggagaacga gggcaagaag gtcttcgtgt
    11821 gcaacggcag cgcctgtttg tgcgcaggca cgcaagacaa gctgcaccac acgctcgaaa
    11881 cgcacttcaa gaaggacgag atcggccaca tctgctgcct cggccgctgc catgagggcg
    11941 cggcgctgca ataccaaggc aaaaactact cggggcagtc cgacgcggca ttgggcgatc
    12001 ttttcagaac cggccaaggt gacagcgaag accgctacgc cgtcgtttcg catctgcagc
    12061 ccgccattct gactgcggag ttcaaaggca tcgaggacta ctacgcgccc ttcaagaagc
    12121 tgatcaccga aggcaatcgg gacgcgctgg ccgccgaatt gaaggacagc ggcctccgtg
    12181 gtcgcggcgg tgccggattc ccgctttcca tcaagtgggc cggctgccgc gcggcggaag
    12241 gcgcggtgaa atatgtcgtc tgcaatgccg acgaaggcga tccgggcgcc tacatcgaca
    12301 aatacctcat ggagaagcag ccgcacagcg tgctgctcgg catgatggtg gccggttggt
    12361 atgccggagc ggagacgggc gtgctctaca tccgcgccga gtatcccgac agcgtcacca
    12421 tcgtgaatca agccatcacg gacctgcatg aggcgggctt gctcggccag aatatcctcg
    12481 gcacaggctt caacttcgct ttgaagtcca tcaaaggcgc cggagcttat atctgcggcg
    12541 aggaaaccgc gctcctcgcc agcattgaag gccagcggcc tgaggtgcgc acgcgtccgc
    12601 ccttcccggt gaatgagggc ctctaccgca agcccaccat cgtcaacaac gtcgagacct
    12661 tcgccaacct ccgcgccatc ctcacggtcg gtggcaaagg ctacgctcaa atcggcacgc
    12721 cacagtccac cggcccgaag ctgctctcgc tcgacagcca ttttgtgaag cccggcatct
    12781 acgaagtcgc catgggcacg ccgttgcaaa ccgtcatcga cctcgcgggc ggcttcaaaa
    12841 cgaagatcaa agccatccaa atcggcggcc cgctcggcgg catcgtcccg atgagccaca
    12901 tcgcccagct cacggtggac ttcgagagct tcaagaaagc tggctttcta ctcggccacg
    12961 ccggtgtcgt cagcatcccg gagacgatgc cgatgatcga atacatccag cacctcttcc
    13021 aattcaccgc cgacgaaagc tgcggcaaat gcttcccctg ccgcctcggc agcacgcgcg
    13081 gcaaggaact catcgccaaa gctcgcggcg acacagcgtt caaaatcgac cgcgaactcc
    13141 tcagcgacct gcttgacacg atggaacaga cctcgctctg cgccctcggc ggcggtgtgc
    13201 cgttgccgat caagaatgca ttggagcatt ttgatgggga gttgaagggc ttttttcgcc
    13261 cgtagttcgc gaatgccccg caattcactc gatcatccac ggcagccagt acgcctgtgc
    13321 catcaccagc agtcccatca gaatggccag cgccacgctg tgccagaaca cgctgcgcag
    13381 caccgtgccg gcatcgggac tgtccggatg accgcctgtc gccacggcgg cgacgacgat
    13441 gctctgcgcg tcaatcatct tgcccatcac acctccactg ctgttcgacg cggccatgtg
    13501 aaccggcgag agatccagtt ggttcgccgt tacttgctgc aagttgccaa agagcacgtt
    13561 cgacgatgtg tcgctgcccg tcagcgccac tccaagccag ccgatgatcg gtgagaaaaa
    13621 cgggaacagc acgcccgtgc ccgccatcat caggcccatc gtcgtgtccg tgccgctgta
    13681 gcgcgtcaca tagccgagcg cgagcatgag cgagatcgtc agcagcggca ctcgtagctt
    13741 ccatatcgtc gccgcataca gtttcgcgat gcgcaccggc cccaatccga gacagaaacc
    13801 cgccagcaca cccgcgagca ccgccgccgt gcccgtggcc gagagcgcgt tgaatttgaa
    13861 caccgccttc tcgaattccg tgtgcaccac gactggcggc acacgttcga ccgccttgtg
    13921 cagcagcggc acctcgatct cctgattgaa ggcggcgttc agtccgttct tcaccgccgg
    13981 catgccccac gcaaagacga atgccgtgag aaaaatccac ggcagccacg ccttccatgc
    14041 cgcgcccaat gcaccacccg cagcttcttc attcgcatgc gcatctgcgg acttcggcct
    14101 ccaaactttc atgaacccaa cgagcgccgc catcgccacg actgcgctca cgatgtccac
    14161 caaccatggc ccgtgatagt tgctcatcac aaactgcatc acgccaaagc tcaatcccgt
    14221 caccaggcac gccggccaca cgccaagcat gccgcgccag cctacctgcg ccgcgaccag
    14281 ccagaacggc acgatgagcg agaacaacgg caactgcctg cccaccatcg cgctgagttg
    14341 catcagatcg agccccgtga tgtccgccag cgtcgtcagt ggcgtgccta gcgatccaaa
    14401 cgccaccggc gccgtgttgc caatgagcgc cagcttcgcc gcttcgagcg gtttaaagcc
    14461 aaggccaatc atgagcgccc ccgtgatcgc caccggcgcg ccgaaaccag ccgcgccctc
    14521 gatgaacgcg ccgaagctga acgcgatcag cagcgcctga acacgccgat cactcgacac
    14581 acccgcgatt tgcttttgca gcacggcgaa ctgacctgtc tcgacgctca gattgtacac
    14641 aaacatcgcg ttcagcacga tccagccgat ggggaacagc ccgaacgccg cgccattcac
    14701 cgccgccatg cccgcaagct gcgccggcat gtgataaacg aagaccgcga tcaaaagcgc
    14761 cgcgaaaagc cccgccagtg ccgcaagctg cgcccgcaca tgccagaacg ccagcaatcc
    14821 aagcagcacc accaccggca gcgccgcgac gagcgacgaa agaataagac tgccgagagg
    14881 atcgtagttt tgggaccagg tcatgatggg agcatggcat cacggatcac ttgcatcgtg
    14941 tgtttcacct gaatcggtga tccgagtttc ttcagatgca aattcacctg cgtcatgcag
    15001 ccgatgttgc cggtgacgac tgcctccgcg ccggtcgcga taatgttctt cgccttctgc
    15061 tcgccgagcg acgacgcgat gtcgggctgg tccatgttgt agctgcccgc actgccgcag
    15121 caaagatgcg cgtcgctgat ttcaagcagt tgcaggcccg gaatggcttt caaaagatcg
    15181 cgcggctgct tgcggacgtt ctgcgcgttc gagagatgac aggcatcgtg gtaggctgcc
    15241 ttgagctttg tttcacgcaa tgattcgcgc agacccagcc tcgccaaaaa cacgctcaca
    15301 tccatcacgc gatgccggaa ggcctccgca cgcgtctcat ccggcgtccc acgaagcacg
    15361 agatggtatt catgcatcgc cgacccgcag cctgcggcgt tcgtgaggat cgcgtccaca
    15421 tcctccggga acgcgccgag attctgccgt gcaaacgaac gcgccgcctc cagatcgccg
    15481 gtgtgccatg cgagacctcc gcagcatccc tgcgagcgcg gaatcaccac ttcgacgcca
    15541 tttcgcgtca gcacctcgat cgtcgccgtg ttgatgtccg gatcgagcac ctgctgcgca
    15601 cagccgagca gcaaggccac ccgtgcgcgc ttttctccac tcgccggata gcattcctcc
    15661 cacgtctgtt tcggcgggag ttcgtccgga atcagttcca gcatcggttt catcatggag
    15721 ggtgccggaa catgctttcc aagccacgcc gccagtcgaa accgccccgg aaacggcacc
    15781 gtctgtgccg ccaaaaaccg ccgcagccgt tccagccagc cgcgtttcat cttccccgcc
    15841 gccaacgcac gaaacggact gatcaaatcg cgatacggca caccgctcgg acacgcgggc
    15901 tcgcacgcga ggcatcccag acagcgatcc acatgcggct gcgcggcctc gaaactcatc
    15961 ttgccctcca gcacctcctt catcagcacg atgcgaccgc gcggcgtgtc catctcctgc
    16021 cccagctcgc gatacgtcgg gcacgccgac agacagaacc cgcaatgcac gcaggccgac
    16081 accgccttcg ccatcggttc gcccagcggg ccgagtttgt cggttggaat ggagtgaagc
    16141 atgttgagcg tcggctttga tatcaatctt ctctgcctgc taatgttttc aattgcaacg
    16201 cccggcggtt caaaagaaaa ggcttccaga ttgtgtcacg gagtgataaa acttgaggca
    16261 agtcatccag caagccatga aactaacact gtcccgcgtc ctgctcctcc ttgtcgccac
    16321 cgtgcttttc ggcgtattca ccccgaatca tcagtcgctt ttcgcagcag gcccgccgaa
    16381 caaagaaccg ggcaaggtgc cgccggatgc tgcaaagggg cgtcaactcg ccgagttcat
    16441 caagcaggcg aaggcgcgtg gcctaaaagg gagtgaactg gcccgcgcca tcgccgagga
    16501 gcgtaagcgc ctaggcatgc cgccggagga tgactgaacc ctgtggcatc agcacggggt
    16561 ggatcagttg ttatcgagag ttggaaaacg gttctgcgga tcgagcgcct gcttcaccgt
    16621 gtcctcgatg gcaaatcgag gccggccgcc gatccacagc ggcgaatcac cgcgcagcgt
    16681 catcccgcga ggcggtggcg tgttcgagag ggcgacgttg ccgccgcagc tcacgtgtgc
    16741 agtcttgaaa tcgttgatct ggtccggtgt gatgtcggtt ttgaaactcc acggcgtttc
    16801 acgaatctcg cgccacaccg cgttgtcgat gatcttaccg ccgatctcct tcgccagagc
    16861 ttcaagtgcc ttggcaggtc cggcaagcct cacgagcatt gtttcgccac cgggcggcac
    16921 gtcgagtgca tcgatctcga aacgcgaatt ggcaagcttg cccatcttct cggccgagaa
    16981 gggcatttgg agcgtgagtg tcgattccgg cttcggaaac accttgaaag tcacttcgcc
    17041 gatagcgccg aatcgtccga ggctgccgac gagaaatttc ggcacatcaa aacccgccgc
    17101 gtttttgacg actttgccgc cgagcttcaa caaacggccc atgccatcga caaagcgcac
    17161 gccgaggatg aaatcgcgaa caccgccgta gcggaagcgc ccgggtccgc ttaatccgct
    17221 cgccaccacg ccgccgatgg tgctgccggc ctcaacgagc atcggatcga agggcaaata
    17281 ctgccctttc gcatccagcg ccgcgatgat ctcgcgcacc ggcgtgccag cgagcgcggt
    17341 gaaggtgaac tcgcttggct catattccgt gatgccgctc aactgccgcg tcgagatctt
    17401 caccgcctcc accgccgaca accgcggctt cgtctccgcg cccacggcga tcacccgcgg
    17461 tgcggagagc acggcgtcgc ggagttcggt gagggtggag ggagcaatca ttcgcgtgaa
    17521 atgacgccgg ccttttccag cgggtgcagg ccgacatggg acaacgaggg cgcttcgctg
    17581 ccggggaaca tcttgccggg attggcgatg cccagcggat cgaaggcggc gcgcagacgc
    17641 tgcatgcagt cgatctcgtc ggcggtgaac atgtcgggca ggaagtcgcg tttctcgaca
    17701 ccgatgccgt gctcgccggt gatggagccg cccatttcga cgcacatctt caggatgcgg
    17761 ccggctaaat cctcggcgcg atgcagcgcg ccttcttcgc ggccatcgaa aaggatgagc
    17821 ggatgcagat tgccatcgcc tgcatggaag acattcgcca cgcggaggcc ggtctcttgc
    17881 gacatctcgg cgatgcggcg cagtgcctcg cccaatcgac gacgcggcac gacgccatcc
    17941 tgcacgatga aatccgggga gagcctgccg acggcgctga aggcactctt gcggcctttc
    18001 caaatggcga gccgctcctc ggcatcgcgt gcgggacgca tctcaaccgg cgcacttgcc
    18061 gcaaggatcg catcaaggcg ttcacgttcg atggcgacga cttcgcgcgg gccttcgagc
    18121 tcgacgatga gcacggcctc gcatcccgga ggatattcgg catgcacggc ggccaccgcc
    18181 gcctccagcg caagcgcgtc catgatttcg atggcaccgg gcagcaatcc gctcgcgatc
    18241 acggcggaca cggcatcgcc cgcctgctcg agcgtgcgat agcccgcgag cacggtgtga
    18301 aagcattcag cctttggcaa aagctgcagc gtgatctcca acgcgatgcc gaatagacct
    18361 tcgttgccgg tgaaaagccc gcaccagtcc ggcccgagcc gttcgcggct gccaccgccc
    18421 cactcgacga tctcgccagt ggcgagcacg gccttgatgc cgagcatgtg attggcggtc
    18481 atgccgtatt tcaggcagtg cgccccgccg gagttgaagg cgacgttgcc gccgatggtg
    18541 cagatgcttt gcgaagaagg gtctggcgcg tagtgcaatc caaacggcgc ggcagccgtc
    18601 gtgacctgcg tgttcaccac acccggctcc accacggcga tgcgctgctc tggatcaagc
    18661 ttcaggatgc ggttgagccg gttcagcgcg atgacgatac cttctgcgat aggcagcgag
    18721 ccgccagaaa ggctcgtgcc gctgccgcgt gcgacaaacg gcagcttcaa ctcatggcac
    18781 agccgcacga tggcgatcac ttcatccgtc gtctccggca ccaccaccgc gagcggtcgc
    18841 gtgcgaaacg cggtgagacc gtcgctttcg taagcggcga gttccaccgg acgcatgagc
    18901 agtcgctccg gcggtaacac tgcagccaga cgatcaagcg cggtggacat gagcgaccgg
    18961 ttatttcagc acgttctgca acgccgtgag gacgagatca acgttccgca cggtcgcgcc
    19021 gtggcccatg aggccgatgc gccaggcttt gccggccatg acgccgaggc cggcgccgat
    19081 ttcgatgccg tattcttcga gcaggcgctt gcgcgtgccc gcgtcgtcgg ctccagcggg
    19141 gatgtggatg cagttgagcg tgtgcagcga gtgtttcggc acgtagctga tgcccatcgc
    19201 ctccagtccg gcacgcaagc gctggtgcat ctgcgcatgg cgtgtgatgc gggcctccag
    19261 tccctcctcc agcacgatgg cgagcgcctc gcgcaaggcg taggtcatgt tgatcggcgc
    19321 ggtgtggtga tacacgcggc cgccaccgcc ggaggtgtag tatttgcgca gcatcgagac
    19381 gtcgagatac caggactgca ccttcgtttt gcgcgagtcc atgatcgcga gcgcacggtc
    19441 gccaaagctg acaggcgcga ggccgggtgg gcagctcagg cacttctgcg tgccgctgta
    19501 gatcgcatcg atgccccagt catcgacttt caattcatga ccgccgagac tggtgacggc
    19561 atcgacgacc agcagcgcgc ccttgctgtg cacgagttct gcaagacctt cgagcggttg
    19621 cagcgcgccg gtgctggtct cggcatgcac gatgccgacg agctttgatt tcggatgctg
    19681 gtcgagcgcg gcggcgattt gatcaaactg aatgacgtca ccccaagcag cctcgaccgc
    19741 atgcacggtg gcgccgcagc gttccatgac gtccttcatg cggccgccga agacgccgtt
    19801 gacgcagacg atcacttcgt cgcccggttc gatgagattc gtggcgatgc attccatccc
    19861 cgccatgccg gtgccgctga cgggaaaggt gagcgagttc ttcgtgcgaa acacctgccg
    19921 cagcatctcg cagacctcgt ccatgatggc gacatactgc ggatcgagat gaccgagcgt
    19981 cggcgcgcca agcgcacgca gcacgcgtga aggaacagga ctggggccgg ggccgagaag
    20041 gatgcgttca gcggggaatt gggtgttcat gtgtgaagct gggaatttga aactgaggca
    20101 aagtgccgat ggagtcgagc ttttatatga tccgcgttcg cgcccccccc ccccccccc
//
LOCUS       DAOMYJ010000081        20080 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Prosthecobacter sp. isolate AMR_MDS_5629
            NODE_936_length_20080_cov_21.029413, whole genome shotgun sequence.
ACCESSION   DAOMYJ010000081 DAOMYJ010000000
VERSION     DAOMYJ010000081.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867850
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prosthecobacter sp. (wastewater metagenome)
  ORGANISM  Prosthecobacter sp.
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Verrucomicrobiaceae; Prosthecobacter.
REFERENCE   1  (bases 1 to 20080)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 20080)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.30
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:34:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,742
            CDSs (total)                      :: 4,684
            Genes (coding)                    :: 4,676
            CDSs (with protein)               :: 4,676
            Genes (RNA)                       :: 58
            tRNAs                             :: 55
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20080
                     /organism="Prosthecobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_936_length_20080_cov_21.029413"
                     /isolate="AMR_MDS_5629"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1965333"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..608
                     /locus_tag="PLP58_10295"
     CDS             <1..608
                     /locus_tag="PLP58_10295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ08951.1"
                     /translation="NRRSLPLLWAPLAIVLLALGLRWLKLESPGMTLLPNFSPWLALV
                     FTGTLLFPRALAWWVWPLLLLGVDVLSQGMDILTPKGGGFEALVMYACYAGAAWLAAR
                     WRGQAGVARTLGGVLACSLGFYIITNSVSWLVEPGYAKSLAGWVQCLTTGLPGYAPTW
                     MFLRNSLLSDMGFSLLLLAAFNAEAHARALPKLRWLAPAAA"
     gene            complement(609..2213)
                     /locus_tag="PLP58_10300"
     CDS             complement(609..2213)
                     /locus_tag="PLP58_10300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ08952.1"
                     /translation="MKAPIMADPAAETTSMTAMEQDLFPHPDPNPAGLTAETSPAWSP
                     DSSKKTNSGVFPRGIQDLADKLRKAMNDPSHLSQAFAEFCTAAYVKPEWAGAASEFLA
                     ELFEGREEHLAEMAHVPDLIIELATGHYAVTFMAASVWAAKGDVARMSRLAEALAATQ
                     SKLSGVEVVDVMLALAASLAISRYPRAEQLVNIAAPQAAPEHEEALAEARLWLAAGRA
                     VRACDQEVRDMWEQRLRRPQHPWTWEGPVERAALTQLADQVAPRMEAESLFQAVLPAG
                     WWELAMRRAEELIHYDAALRQKKCETHEDAPPPAEPPPMVIGRPIVIWRLVPFCFGGL
                     VGAWAFALGIYFGPFDLVRPGASVNEVGAAADGQTTPGGEFSQPGEEGEPPSASVSAD
                     VRQGEHPDSLWRKEESRRLLEKLPELAASGELLKHGGWEQHRAMLEGEEENLSKNSPE
                     YEKLLVWLHLSPVSDDAINTRVPGLLAALKPDSDTLDLWEKLIYPGSPNAPQIREAAR
                     RQHHENTQSWSPTQRSRLSRIGWDTP"
     gene            complement(2281..3642)
                     /locus_tag="PLP58_10305"
     CDS             complement(2281..3642)
                     /locus_tag="PLP58_10305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009963309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1552 domain-containing protein"
                     /protein_id="HRJ08953.1"
                     /translation="MTPFHETASASHQMSRRKVLRGLGTLMSLPFLESAGGRAFAAAP
                     PQKAPLRAAWLYIPNGVNVHKWFPTGEGAGYQLSETLKQIERHRNDFMAVSGLAQDKA
                     RSHGNGGGDHARATSTFLTGCMPKKTAGADIKLGVSVDQIAAEKIGHLTRLSSLELST
                     DGQRSSGRCDSGYSCAYQYNLAWKNETMPMAPEMDPRLVFERLFGYGASGARGEEGAR
                     RQRLQKSILDTVLGEAKSLQGKVSGSDRRKLDEYFESVRDIEQRIERSEKFAAQLPPG
                     VSVPEGIPESYEEHIRVMFDLLALAFQTDTTRLCTFLMAHDGSNRSFPQIGVPDAHHY
                     LSHHENDPQKLEKIAKIDQFYMRQFGYFLDKMKSVKEGDSTLLDNSMIVFGGGIGDGN
                     RHNHDDLPILLAGRAGGTWSPGKRIVLKEETPMTNLYLSMLDRLGVRADRVGDSTGVL
                     DVG"
     gene            3791..4276
                     /locus_tag="PLP58_10310"
     CDS             3791..4276
                     /locus_tag="PLP58_10310"
                     /inference="COORDINATES: protein motif:HMM:NF012355.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="plastocyanin/azurin family copper-binding
                     protein"
                     /protein_id="HRJ08954.1"
                     /translation="MPKLSKIAVIALAATVLHAQEAEVAEIELKPHASNPLGYDKTDL
                     AVKVGQKIKLTLNNTGSIVPQPHNFLLLRKGKMPDNKDVIDAVGAQANLMMGDPQGMA
                     KHYIPEASKADILAHTKLVQANGSDTIEFTAPAEEGDYAYICTFPGHWLLMRGVMHVT
                     K"
     gene            4297..4818
                     /locus_tag="PLP58_10315"
     CDS             4297..4818
                     /locus_tag="PLP58_10315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ08955.1"
                     /translation="MKHMILLLALFAGHAQTSSAEEKKVSAGGLDFVLPSAAWDSVPT
                     SSPMRAATLKISVEGAEQPLEAVFYYFGAGQGGDVEANVQRWFGQFEGKPETKREDIE
                     AGGKKVTLVTATGTYMDGAMFGPKTPKPDSMLLGSIIPGPDAPVFIKLTGPKAAVAKV
                     ADEFRALSVSPFK"
     gene            4950..5369
                     /locus_tag="PLP58_10320"
     CDS             4950..5369
                     /locus_tag="PLP58_10320"
                     /inference="COORDINATES: protein motif:HMM:NF012780.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HRJ08956.1"
                     /translation="MKRHEPVTRVMSRHLVTVHHGDPVSKIRHLVREHGVHHIPVVSG
                     DQLLGIISWSDILRVSFGDAFNTDERAVDATLDHTLTIEELMCKTPVTLPDTATVRDA
                     AKILARGEFHSVPVVNGGKLCGIVTSTDLIKHLLEQF"
     gene            5424..5909
                     /gene="ruvX"
                     /locus_tag="PLP58_10325"
     CDS             5424..5909
                     /gene="ruvX"
                     /locus_tag="PLP58_10325"
                     /inference="COORDINATES: protein motif:HMM:NF015607.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HRJ08957.1"
                     /translation="MPRILAIDHGTVRIGLALSDELELTARPLKTLDARAEPEREIAR
                     IARDKRVGRIILGMPFHLDGRRGEAAERVEKFAESLGRSLRHEIPLEFVDERLSSVEA
                     EAALSRAGITGKKQRDEVVDQLAAVVILQEYLNSQRGPEGFLLPEECQNLEWTHEGRR
                     H"
     gene            5890..6732
                     /gene="truA"
                     /locus_tag="PLP58_10330"
     CDS             5890..6732
                     /gene="truA"
                     /locus_tag="PLP58_10330"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HRJ08958.1"
                     /translation="MREDGIESKLGLHPAAPQGMRRLRLTVAYCGSGWRGWQSLAGGG
                     TIQDELTAAVKKATRIETVVHGSGRTDAGVHALAQVAHMDVPASARMSHGAWCKALNA
                     CLPMTIRVTRVEDAEPGFHARFDAVGKIYRYRIWRPEMLDPFEADRAWHVYGRLDISA
                     LRRCADLLAGRHNFVRLSANRGDMWETQRRALPEKTTRTLHRVDIAEKGDVLEIEFEG
                     DGFLYRMARLITGSMVQVARGRATEEWFASLLATHEGLQSNQMAPACGLYLVSVKYAG
                     DLRH"
     gene            complement(6776..8029)
                     /locus_tag="PLP58_10335"
     CDS             complement(6776..8029)
                     /locus_tag="PLP58_10335"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hybrid sensor histidine kinase/response
                     regulator"
                     /protein_id="HRJ08959.1"
                     /translation="MQNTHDYKRYAVLYVDDEEMALKYFQKTFGAEFRVLTAINAVEG
                     LNYIESMGEEIGVLLTDQRMPGQKGVQLLERARQLRPKMVRMMITAYADFGVSVDAIN
                     LGNIFRYISKPLQVEDMRNTLRRAMDFYMLQKERDDLLREKLSVLQSMVITDRVISLG
                     VLASGLCKNLRNPLDAVNTFLNLVPARLRQENVELDRLRDPAFWRDFHNQVLAQTQRI
                     SDSIGDLDKISSQAPADIAPEDVVAQAVDTVRAAAAAKKITLKNEVQGPLPKLHAAPD
                     RFARIFEMLLKDQVELLDPGAEITLSASVSKVSDTPVGIRFEIKDNGPGLPQEALRSI
                     FDPFHIRPDGEEKFGLDLLGVYFLVHHHGGHIEAGSSPGEGVTYTMDIPLLSSPENGA
                     TSSSRDFVTKVLMNDVLWERLLAGD"
     gene            complement(8048..10732)
                     /locus_tag="PLP58_10340"
     CDS             complement(8048..10732)
                     /locus_tag="PLP58_10340"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HRJ08960.1"
                     /translation="MPETSTVSVEELERSFNEYERPIRIRNYKLGALLAAVFMPAGAV
                     LDFFVYGWEGVVRFAPARILTTAFLAFVGVALIKFPQCRHYRALGFLVALIPLLAIAW
                     MIYAEQGTASPYYAGLNLVMLGSAILLRWSSMDSVIIFLLTFLAYILATVMHEPFQFD
                     GLYFNNLYFLFVTGVFIIAGSWYYNSIRQSEFELRWRLDINRAQLEESNQKLRELDEL
                     KSRFFANISHELRTPLTLLIAPLETLLSRGRVMAAAEMDEMLATMHGNAMRLLKLIND
                     LLDLVRLESGKIEIKKAAVPIEDFLDGIVNAVSGVASDKHIALRSWCQDGIGRVMLDA
                     DKLERICLNLIFNALKFTPAGGQVNITAEKEEGWLRIEVKDTGVGMSREQLKRIFTRF
                     WQADTSSRRKFQGMGIGLALVKELSEAHGGSVEAVSEEGKGTTMIVRLPLETAPPDAP
                     EPEPNFEAASVEQLPEDRGEWISELYRRAELFPAITSLQATMRPIETGISRRSKKPKL
                     LIADDEPDMLRFLRSQLGTFFDVLEAVDGNQVVEKATQFLPDIVLTDMMMPEKDGLQV
                     CRELRDRTSTRSIPVVLLTARADEKTKIDCLEAGASDFLAKPFSLTELCVRLKNLVDN
                     RLYQKQLVEQKQQLESAFEQIKETEALIIRNEKLASLGKLSAGLIHEINNPLNYAKQG
                     LYGLRRIQDVLPEAEKADFVETLGDIEHGVDRVARIIADLRGFSRTTREMNASFPLKS
                     VVETTMRFFGQEFKEGVGLEMDIPDDLEIRGDSNQIVQVLINFTQNALDAMRTKTYPE
                     GEGPAIRISAENLPGRTLVRFRDNGPGIPEDMRSRIFDPFFTTKDVGEGMGLGLSICH
                     RIIADHGGHVDIHSREGAYCEFVLEFPLVETNLSASEP"
     gene            complement(10732..12171)
                     /locus_tag="PLP58_10345"
     CDS             complement(10732..12171)
                     /locus_tag="PLP58_10345"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HRJ08961.1"
                     /translation="MEQNAPTPLPLNTDAAERISMVVCRNSQGAEVRATLHRLTRYMA
                     VFEVYNPYSIVQLSEVLNDFRVTMNERVVYSGRAVIANLVNTGIMLICEASLTEEGWI
                     DFVSPTADKEKLLSEFRDFLRETERIYRVSPEFKLVVADMQTLLTDLRRWMEQVELGV
                     RSLPTGDRIATERETIQQLEVPIMPAVAPLMERFESNASVIPVEQQPIHRSYIKRQLH
                     PLVLCSPFIYRTFQKPLGYAGDYEMVSMMLRDPYEGSSMFAKLLNRVFLDTAPVVAHR
                     NRITYLIERLRDETARAAGEGRVARIHNLGCGPAQEVQSFLNTDSISDEADMVLLDFN
                     DETLVNTTRVLEDIRARHRRRTGLKFEKKSVHQILKEAGRPAPGQKGYDLVYCAGLFD
                     YLSDRICRRLLEIFYDMTAPGGLVVATNVDSSNPSRNWMEYVLEWNLVYRDRESFLKL
                     APEKAPRGSVDIKADSTGVNIFLEIRKPA"
     gene            12388..13278
                     /locus_tag="PLP58_10350"
     CDS             12388..13278
                     /locus_tag="PLP58_10350"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HRJ08962.1"
                     /translation="MQLMPTNEAPVIGEPVQFESLGETICALFVSSIRPVGQPVLVVS
                     HGAGEFKENYLELASYLADRGIASLLLDMHGHGASGGRPFHVSMREWVSDISAALDYL
                     ETRVDVDPERIGALGLSSGGSAILEAAVCDSRLKALIPLDATVMNTLPAGVSLTMRLL
                     SLIGWVKRMLTGKDLRISIIKLLDEVPMASDPAINARLKVDPGKLKAFMNFPMPGAAD
                     AFFVDTIRRVPAIKAPTLVIWGADDQLDPVSTAHILFQALSCEKGLEIIEGNGHVGHL
                     DRNRVRVFEVATDWLLKYLA"
     gene            13322..14449
                     /locus_tag="PLP58_10355"
     CDS             13322..14449
                     /locus_tag="PLP58_10355"
                     /inference="COORDINATES: protein motif:HMM:NF021445.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2156 domain-containing protein"
                     /protein_id="HRJ08963.1"
                     /translation="MRLILGQEAKTLEPAEKWKLLSPALRQHGSQAMSYACLQQGMEY
                     FVHELGFIAFITVKHPVFARTPRRIALTDPVCAEEDMPALVAAFLESHPSAVFSVASE
                     FAADAMRRLGFKANCVGYEPELPVQTYNTAGNWKELDMIKRARNEARREGITIREVDI
                     ATVPEEQLQALSQNWIQGKKVNDREIWIYARRPVYQSEPDVRKFVAFDRNGRAAGYVF
                     YDPMYRDGRVFGYSANTVRCDERQFGRLATAIHMTAMDVFRPEGVDVLNLCLCPFTHL
                     QLGRYRDDLATDWFFQVSRRYGGEIYNFDGLSFHKSKYRGREKPIYFVSNSALPSNDV
                     YLAFLAADIATGYFSTMGLLARGIWKAVRGRKGRGAGGQSG"
     gene            complement(14446..15828)
                     /locus_tag="PLP58_10360"
     CDS             complement(14446..15828)
                     /locus_tag="PLP58_10360"
                     /inference="COORDINATES: protein motif:HMM:NF016830.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate porin"
                     /protein_id="HRJ08964.1"
                     /translation="MNPSLSLNFVFFFAFVVAPLCATENAEPSGGSPIERWFMQDYML
                     GDWGGLRSDLSDHGVDLEFFYVGSVPNNISGGIRHGAIYQSGMILALDLDFEKMGLWK
                     GGSFHASSVWLNGQPFSDQYVGDLNKTNLVDFPADFRVWQVYFQQHLLDEKLTLKAGL
                     LSVDRDFILPEMYNSLASINFLNQTFFFPTMAFNLYDQPFFPKGAHSLPSTPYGVPAV
                     VLKWDASQSLYLKAGVYDGYPDKTGPGTRIQLHSDEGALFFYELGWRRNMEQGAKGLP
                     TSVKAGGYYHTDEFYDIYNTIASLIPLGLSNGPTTHDGNYGLYLLAERMLWREQGPDD
                     PAQQGVTGFFRTAWAPRDRNIAEYSIDGGLVFKGLIPGRDYDTLGIAGSYMRMSRDLE
                     RAVEDINKVLGFQFAEPHDYEAVVELSYKLQLAAWWTLSPNLQYVMHPGGSGAIPNAW
                     VFALQTTLRF"
     gene            16014..19592
                     /gene="dnaE"
                     /locus_tag="PLP58_10365"
     CDS             16014..19592
                     /gene="dnaE"
                     /locus_tag="PLP58_10365"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF004226.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HRJ08965.1"
                     /translation="MPDSFVHLHVHSDFSLLDGACRISDLVKRAGKLGMPAVALTDHG
                     NLYGAIDFYMAAKKAKLKAILGCEVYLAPGSMREHKDVPGRQRASHLTLLAENLAGYE
                     NLSRLVTKSHLDGLWLDTPRIDKDTLREHREGIICLSGCMSGEINELLHSGRPEEAEH
                     SLREFRDIFGPDHFFLEFMDHGLEQQQHSTRQLVEWGKKYGLKTVATNDVHFLRREDH
                     ESHDILIRIGLGATVHDTNLRTYSPEVHFKTAEEMRALFHELPEACDTTLDIADRCNL
                     DIRLDSTSIDRYPTYHPPDGSDRNDYLRRLCEEGLIRRYGRDRALNDETLRQRLEKEL
                     SLLSEKNFTSYFLIVWDFINWAREHGIPVGPGRGSAAGSVVAYVLGITNLDPLRFELV
                     FERFLNPERVSPPDIDIDFCQTRRPEVIEYVRQKYGERSVSHIITFGTMGAKSVIRDV
                     GRVLGWSYGDADRLSKMIPTELNITLQGAEEKDKEGKTSFKPGAIDKNPELKAYVETD
                     GSAQELWRHALFLEGLTRGTGIHAAGIVIGDKPLSEFIALTRGNEGEIVSQYAMKPLT
                     ELGMLKMDFLGLKTLTVIHEAEHWVRKRVPGFDTGQVSLDDTATFELLKRGDTVAVFQ
                     MESGGMAATCKNLGPDTIEEIIAIIALYRPGPMQFIDDYIARKKGLQKVEYPHPLLEP
                     IAKETYGILVYQEQVQQAANILAGFSLGQADILRRAMGKKDAQEMARQRVIFVEGFIK
                     HRGVPEKQAHAVFDLLEKFAQYGFNKSHSAAYGLVTYHTAYLKTNYPVEFMAAVLSYE
                     ISNTDKIAFFVSECQRMGITILPPDINKSSLKFAPETFASTETPPAVPGIPNAIRYGL
                     SGIKNVGEAAMAAAIEERRKNGPFHSLDDFASRMDTRAVNKRMLEALVKCGAFDFTGE
                     PRPVMFARLDPVLAAASALQKDRQSGQGGLFDDLGAAPPQKTPARHTAVESWSQDEIL
                     AFEKELLGFYVSGHPLDKYRGHFDSPRIHRIGALDEVDTAAKPVSLFILGIIKTLDIR
                     YSKKDNRPFCTFTIEDFSGPAEVMAWSDEFEKFKDLLKPGAVLGMRVRCAKDQRGDGN
                     RVTLSGDVREAKPKKPRDPNAALPAAPATPAPAPPKPLLLRLDCRRHSQIDLDRIQEV
                     LAAHPGTLPVIFEFLRRDGTRLRFQAGDEYRVTQTRELVQALMIWM"
     gene            complement(19710..>20080)
                     /locus_tag="PLP58_10370"
     CDS             complement(19710..>20080)
                     /locus_tag="PLP58_10370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009960052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HRJ08966.1"
                     /translation="ARLGLAEHLRKRPAELSGGQQQRAAIARALAPRPRLLLLDEPTS
                     ALDPVMTGEVLDVIRELAEGGQQIVLATHEISFARKVADWVVFLAAGKVAESQSAAGF
                     FSQPATAQAREYLDGLSRYR"
BASE COUNT         3959 a         6342 c         5981 g         3798 t
ORIGIN      
        1 cgaaccgccg ctccctgccc ctgctatggg cccctctcgc catcgtgctc ctggcgcttg
       61 gcctgcgctg gctgaagctg gagtcccctg gcatgacgct gctgccgaat ttctcgccct
      121 ggctggcgct ggtgttcacg ggcaccctct tgtttccgcg cgcgctggcc tggtgggtgt
      181 ggccgctgct gctgctggga gtggatgtgc tctcgcaggg catggacatt ctcacgccca
      241 agggcggcgg ctttgaggcc ctggtcatgt atgcctgcta tgccggcgcg gcctggctgg
      301 cggcgcgctg gcgcgggcag gcgggtgtgg cgcggacgct gggcggtgtg ctggcctgct
      361 cgctgggctt ttatatcatc acgaacagcg tgagctggct cgtcgagccg ggatacgcga
      421 aaagccttgc cggctgggtt cagtgcctca ccaccggcct gcccggttat gcgcccactt
      481 ggatgttttt gcgcaattcc ctgctgagcg acatgggctt cagcctcttg ctgctggccg
      541 ccttcaatgc cgaggcgcat gcgcgcgcgc tgccaaagct gcgctggctg gctccggcgg
      601 cggcgtgatc agggagtgtc ccagccgatt ctgcttagcc gcgaccgctg agtgggcgac
      661 catgactgcg tgttctcatg atgctggcgg cgcgcggcct cgcgaatttg cggggcattc
      721 ggtgatcccg ggtagatgag tttttcccaa aggtcgaggg tgtcgctgtc gggtttcaaa
      781 gcggcgagca ggccgggaac gcgtgtgttg atcgcgtcat ctgacactgg agagagatgc
      841 agccagacga gcagtttttc atattcaggc gagtttttgg aaaggttctc ctcctcaccc
      901 tccagcatgg cgcgatgctg ctcccaaccg ccgtgtttga gcagctctcc actggcggcc
      961 agttcaggca gcttttccaa aaggcggcgg ctttcctcct ttctccaaag ggagtcggga
     1021 tgctctccct gcctgacatc cgcggaaacg gatgcagacg gcggctcccc ttcctccccg
     1081 ggttgcgaga attccccgcc cggggtcgtc tgcccgtctg cggcagcccc gacctcgttc
     1141 acagacgcgc cggggcgcac caaatcaaac gggccaaaat agattccaag cgcaaaagcc
     1201 caggcaccga ccaggccgcc gaagcagaag ggcaccagcc gccagatgac aatcggcctg
     1261 cctatgacca ttggcggcgg ctcggcggga ggaggagcat cttcgtgtgt ctcgcatttt
     1321 ttctgtctca aagcagcatc gtaatggatc aactcctcag cgcggcgcat ggcaagctcc
     1381 caccagcccg ccggcaagac ggcttggaac aaactctccg cctccatcct gggcgcgact
     1441 tggtccgcca attgtgtcag cgcggcccgc tccacggggc cttcccaggt ccatgggtgc
     1501 tgcggccggc gaagacgctg ctcccacatg tccctcacct cctggtcgca ggcgcgaacg
     1561 gcacgccccg cagccagcca gagacgagcc tcagccaaag cctcctcatg ttcgggggca
     1621 gcctgtggcg cggcgatgtt cacgagttgt tcggcgcggg gatagcggga aattgccagc
     1681 gatgccgcga gcgccagcat cacatccacc acttccacac cggagagctt ggattgtgtg
     1741 gctgccagcg cctcggcgag gcgtgacatg cgcgccacat cccccttcgc agcccagacg
     1801 gaggcggcca tgaaagtcac cgcatagtgg cctgtggcca gctcgatgat caagtccggc
     1861 acatgcgcca tttctgcgag atgttcctcg cgcccttcga acagctccgc caaaaactca
     1921 ctggcggcac ccgcccactc cggcttcaca taggcggcgg tgcaaaactc ggcgaaagcc
     1981 tgggacaaat gcgacgggtc attcatcgcc ttgcgcaatt tgtcggcgag gtcttgaatc
     2041 ccgcgaggaa agactcctga gttcgttttt ttcgaggagt caggagacca cgcaggcgag
     2101 gtttccgcag tcaagccggc aggattcgga tcaggatgcg gaaacaaatc ctgctccatc
     2161 gcggtcatgc tggtggtctc ggctgcgggg tccgccatga taggcgcttt catataacca
     2221 atgcgcgcgg cttgcaacgc tgctgttgca aaagcggccc caaaatgccg cgcgcctgcc
     2281 tcaacccacg tcgagcacgc ccgtgctgtc gcccacacga tcggcacgaa cgccgagacg
     2341 gtccagcatg gagaggtaca agtttgtcat cggggtttcc tctttcaaaa cgatgcgttt
     2401 gccgggactc caggtgccgc ctgcgcggcc ggctagcagg atgggcagat cgtcgtgatt
     2461 gtggcggttg ccgtcgccaa tgccgccgcc aaaaacgatc atgctgttgt cgagcagggt
     2521 agagtccccc tccttgacgc ttttcatttt gtcgaggaaa tagccgaact gacgcatgta
     2581 gaactggtca attttggcga tcttctccaa cttttgcggg tcgttttcgt ggtgggagag
     2641 ataatgatgg gcgtccggga cgccgatctg cgggaagctg cggttgctgc catcgtgcgc
     2701 catgagaaag gtgcagaggc gggtggtgtc cgtctggaaa gccagcgcca gcaagtcaaa
     2761 catcacacga atgtgctcct cgtagctctc agggatgccc tccggcacgg acactccggg
     2821 agggagctga gcggcgaatt tttcggagcg ctcgatgcgc tgctcaatat cgcgcacgga
     2881 ctcgaagtat tcgtcgagct tgcgacggtc gctgccgctt accttgccct gcagggactt
     2941 ggcttctccg agcaccgtgt cgaggatgct tttctgcaga cgctggcggc gagcgccctc
     3001 ctcgccgcgc gcgccggagg cgccgtagcc gaagagccgc tcgaagacga ggcgcgggtc
     3061 catctcgggc gccatgggca tggtctcgtt tttccatgcc agattatatt gataggcgca
     3121 ggagtagccg ctgtcgcagc ggccggagct gcgctggccg tcggtgctca actccaggga
     3181 ggagagacgg gtgagatggc cgatcttctc agccgcaatc tggtccacgg agacgcctag
     3241 cttgatgtca gcgcccgccg ttttcttcgg catgcagccg gtgaggaagg tggaggtggc
     3301 gcgcgcgtgg tcgccgccgc cgttaccgtg ggagcgggct ttgtcctggg ccaggccgga
     3361 gacggccatg aagtcattcc ggtgccgctc aatctgcttg agcgtctcgc tgagctggta
     3421 tcccgcccct tcgcctgtgg ggaaccattt atggacattg actccgttcg gtatgtagag
     3481 ccaagccgcg cgcagcggag ctttttgcgg cggcgcggcg gcgaaagccc tgccgcccgc
     3541 ggactcgaga aagggcagtg acatgagcgt gccgagcccg cgcagcactt tgcggcggct
     3601 catctgatga gaggcggagg cggtttcgtg aaacggagtc atgagtgggg gtgataccat
     3661 ttacgcccgg caggcgaggg tactttcccc aacttgcgga ccgcactctc cgcgccagtt
     3721 ccaacaaaaa agttgaaaac acccggaact ggctgccgat tagcctaccc ccttattcaa
     3781 tatcaatacc atgcccaaac tctccaagat cgctgtcatc gccctggctg ccaccgtcct
     3841 ccatgcccag gaggctgaag ttgccgagat cgaactcaag ccccacgcta gcaatcccct
     3901 cggctatgac aagaccgacc ttgccgtcaa agttggacag aagatcaagc tgaccttgaa
     3961 caacacgggc agcatcgtcc cgcagccgca caatttcctg ctgctcagga agggcaagat
     4021 gcctgacaac aaggatgtga tagacgcggt gggtgcccag gccaatctca tgatggggga
     4081 tccccagggc atggccaagc attacatccc ggaggcgtcc aaggccgaca tcctcgcgca
     4141 caccaagctc gtccaggcca atggctccga cactattgaa ttcactgctc ccgccgagga
     4201 gggggactat gcctacatct gcacctttcc cggccactgg ctgctgatga ggggtgtgat
     4261 gcatgtcacg aaataaattc cccaaacatc ccagccatga aacacatgat cctccttctg
     4321 gccctgttcg cgggccacgc gcaaacctcc tccgccgagg aaaaaaaagt cagcgccggc
     4381 ggcctggatt tcgtcctgcc atccgccgcg tgggactccg tgccgacgag cagcccgatg
     4441 cgcgccgcca cactgaagat cagcgtcgaa ggcgcggagc agcccctgga ggcggtcttt
     4501 tactacttcg gcgccggcca gggcggcgat gtcgaggcga atgtgcagcg ctggttcggc
     4561 cagttcgagg gcaagcccga gaccaagcgc gaggacatcg aggccggagg caaaaaagtg
     4621 accctcgtca cagccaccgg cacctacatg gacggtgcca tgttcggacc gaagaccccg
     4681 aagccggact ccatgcttct aggttccatc attcccggac ccgacgcccc tgttttcatc
     4741 aagctcaccg gcccgaaggc tgccgtggcc aaagtcgccg acgagttccg cgctctttcg
     4801 gtcagcccgt tcaaataaga cgcttcttca aactccgagg gcgggccgtc aggtccgccc
     4861 tctttttttc gggcaagaat cagccggtga acggcaaggc acgatgagca cggccgccct
     4921 gcaaaggcca gtatggcagt catccaacca tgaaacgcca cgaacccgtc acccgagtca
     4981 tgagccgcca tctggtcacc gttcaccacg gtgaccccgt ctcaaaaatc cgccacctcg
     5041 tccgcgagca tggcgttcac catattcctg ttgtcagcgg cgaccagctt ctcgggatca
     5101 tttcctggag cgacattctg cgcgtcagct ttggcgatgc cttcaatacc gacgagcgcg
     5161 ccgtggacgc cactcttgat cacacgctca ccatcgagga gttgatgtgc aagaccccgg
     5221 tcaccctgcc cgacacggcc actgtccgcg atgccgccaa aattcttgcc agaggcgagt
     5281 ttcattccgt gccggttgtc aatggcggca agctctgcgg cattgtcacc agcactgacc
     5341 tcatcaaaca cctccttgag cagttttgat ttgatcgagg gattgaagct ggaaaacggg
     5401 cgcttccggg caagaatgaa gtcatgcccc gaatcctcgc cattgaccac ggcaccgtcc
     5461 gcatcggact cgcgctgagc gacgagctgg agttgacggc ccgcccgctc aaaacgctgg
     5521 acgcgcgcgc ggagcctgag cgcgagatcg cgcgcatcgc acgggacaag cgcgtgggcc
     5581 ggatcattct cggcatgcct ttccacctgg acggcagacg cggagaggcg gcggagaggg
     5641 tcgaaaaatt tgccgagtcg ctgggcaggt ccctgcgcca tgagattccg ctggagtttg
     5701 tggacgaaag gctctcctcg gtggaggcgg aggcggcgct ttcccgcgcg gggatcaccg
     5761 gcaaaaagca gagggacgag gtggtggacc agctcgccgc agtggtgatc ctgcaggaat
     5821 atctgaattc acaacgcggg ccggagggtt ttctgctgcc ggaggaatgc cagaatctgg
     5881 agtggacgca tgagggaaga cggcattgaa agcaagcttg gcctgcaccc cgccgcgcca
     5941 caaggaatgc ggcgcctgcg cctcacagtg gcctactgcg gcagcggctg gcgcggctgg
     6001 cagagcctcg cgggcggtgg cacgatccag gacgagctga ccgccgctgt aaaaaaagcc
     6061 acccgcattg agaccgtggt gcatggctcc gggcgcaccg acgcgggtgt gcatgcgctg
     6121 gcgcaagtgg cgcacatgga tgtgcctgcc agcgcgcgca tgagccatgg cgcatggtgc
     6181 aaggcgctga acgcctgcct gccaatgacg atccgcgtca cacgggtcga ggacgcggaa
     6241 ccgggctttc acgcgcgctt tgacgcggtc ggaaaaatct accgttaccg catctggcgt
     6301 ccggagatgc tggacccttt cgaagcggac cgcgcctggc atgtctatgg ccggctggac
     6361 atctccgcgc tgcggcgctg cgcggacctg ctggcgggga ggcacaactt tgtccggctt
     6421 tccgccaacc gcggtgacat gtgggaaacg caaaggcggg ccctgccgga gaagacgacg
     6481 cgcactctgc acagggtgga cattgccgaa aagggcgatg tgctggaaat cgagttcgag
     6541 ggagacggct tcctttaccg gatggcgcgc cttatcacgg ggagcatggt ccaggtcgcg
     6601 cgcggacggg ccaccgagga gtggttcgca agtttgctgg ccactcacga ggggctgcaa
     6661 agcaatcaga tggctccagc atgcggcctc tatctggtgt cggtgaagta tgccggagac
     6721 ttgcggcatt gactgccgcg cggggcaacg ccgcctcttt cggcggactg gccgctcagt
     6781 ctcccgcgag cagtctttcc cacaggacgt cgttcatgag cactttggtg acaaagtcgc
     6841 ggctgctgga agtggcgccg ttttccggcg aacttaacag cgggatgtcc atggtgtaag
     6901 tgacgccctc acccggcgag ctgcccgcct cgatgtgacc gccgtggtgg tggacgagga
     6961 aataaactcc gagcaggtcg aggccaaatt tctcctcccc atccgggcga atgtggaaag
     7021 ggtcaaagat ggagcgcagc gcctcctggg gcaggccggg gccgttgtcc ttgatctcga
     7081 atcgaatgcc caccggtgtg tcggagactt tgctgacact ggcggagagt gtgatctcgg
     7141 cgcccgggtc aagcaactcg acctggtctt tgagcagcat ttcgaagatg cgcgcgaagc
     7201 ggtctggagc ggcgtgcagc ttgggcagcg ggccctggac ctcgtttttg agggtgattt
     7261 ttttggcggc ggcggcggcc ctcacggtgt ccacagcctg cgccacaaca tcctctggag
     7321 cgatgtcggc gggagcctgg ctgctgattt tgtcgagatc accgatcgag tcggagatac
     7381 gctgggtctg cgccagcact tggttgtgaa agtcacgcca gaaagcgggg tcgcgcaggc
     7441 ggtcgagttc cacgttttcc tgacgcaggc gggccgggac gaggttgagg aaggtgttca
     7501 ccgcgtcgag gggattgcgc aggtttttgc acaggccgga ggcgagcacg ccgaggctga
     7561 tgacgcggtc ggtgatgacc atgctctgga gcacggagag tttttcacgc agcaggtcgt
     7621 cgcgctcctt ttgcagcatg tagaagtcca tggcccggcg cagggtgttg cgcatgtcct
     7681 cgacctggag gggcttggag atgtagcgga agatgttgcc gaggttgatg gcatccacgg
     7741 agacgccgaa gtcggcgtag gcggtgatca tcatgcggac catcttgggg cggagctggc
     7801 gggcgcgttc gaggagctgc actcccttct ggccgggcat gcgctgatca gtgagcagga
     7861 cgccgatttc ctcgcccatg ctctcgatgt aattgagtcc ctcgacggcg ttgatggctg
     7921 tcaggacgcg gaattcagcg ccaaatgttt tttggaaata cttcagagcc atctcctcat
     7981 catccacata gaggacggca tagcgtttgt agtcgtgggt gttttgcatg gtgtttaggt
     8041 tttcgggtta tggctcgctg gcggaaaggt tcgtctcgac aaggggaaac tccaacacga
     8101 attcgcaata tgctccctcg cggctgtgaa tgtcaacatg accaccgtgg tcggcaatga
     8161 tccggtggca gatggacagg ccaagtccca tgccctcacc gacatccttg gtggtgaaaa
     8221 acgggtcaaa aatgcggctg cgcatatcct ccggaatgcc ggggccgttg tcacggaagc
     8281 gcacaagagt gcggccaggc agattttccg ccgagatgcg gatggcgggc ccctcgccct
     8341 ccgggtatgt cttggtcctc atggcatcga gggcgttttg ggtgaagttg atgagcacct
     8401 gcacgatctg gttgctgtcg ccgcgaattt cgaggtcatc tgggatgtcc atctccagac
     8461 cgacgccttc tttgaactcc tgcccaaaga aacgcatggt ggtttcgacc acggatttga
     8521 gaggaaatga ggcgttcatc tcgcgggtgg tgcgtgagaa gccccgcaag tcggcgatga
     8581 tgcgcgccac gcggtccaca ccatgctcaa tgtcgccgag agtctcgaca aagtcggcct
     8641 tctccgcctc cggcagcaca tcctggatgc ggcgcaggcc gtaaaggccc tgcttggcat
     8701 agttgagggg gttgttgatc tcgtggatga gaccggcgct gagcttgccg agggatgcca
     8761 gcttttcatt gcggatgatc aatgcctcgg tctccttgat ctgctcgaaa gcggattcga
     8821 gctgctgttt ctgctcgacg agctgctttt gatagaggcg gttgtccacg aggtttttga
     8881 gccgcacaca gagctcggtc agcgagaatg gcttggcgag aaaatcgctg gctccggctt
     8941 ccaggcagtc aattttcgtc ttttcatcgg cgcgggctgt gagcagcacg actgggatgg
     9001 agcgtgtgga tgtgcggtcg cgcagctcgc ggcagacctg caacccgtcc ttttcaggca
     9061 tcatcatgtc ggtgagcacg atgtcgggga gaaactgggt ggctttttcg acgacctggt
     9121 tgccatccac ggcctcaagg acatcaaaaa acgtgcctag ctgggaacga aggaaacgca
     9181 gcatgtccgg ctcgtcgtcg gcgatgagca gttttggctt tttgctgcgg cggctgatgc
     9241 ccgtctcaat gggccgcatg gtggcctgaa gggaggtgat ggcggggaag agctcggcgc
     9301 ggcggtagag ctcgctgatc cactcgccgc ggtcctcggg caattgctcg acggaggcag
     9361 cctcgaagtt cggttcgggt tcgggcgcgt ccggcggggc ggtttccaga ggcaggcgca
     9421 cgatcatcgt ggtgcccttg ccctcctcgc tgacggcttc caccgagccg ccgtgcgcct
     9481 cggacaattc cttgaccagc gccaggccga tccccatgcc ctggaacttt cggcgggagg
     9541 aggtgtcagc ctgccagaag cgggtgaaga tgcgcttgag ctgctccctg ctcatgccga
     9601 cgccggtgtc cttgacctcg atgcgcagcc agccctcctc cttttcggcg gtgatgttga
     9661 cctggcctcc ggcgggcgtg aatttgaggg cgttgaagat gaggttcaga cagatgcgct
     9721 cgagcttgtc ggcgtccagc atgacacgtc cgatgccgtc ctggcaccag gagcgaaggg
     9781 cgatgtgctt gtccgaggcc actccgctga cggcgttgac gatgccgtcg aggaagtcct
     9841 caatcggcac ggcggctttt ttgatctcga tcttgccgga ctccaggcgc acgaggtcga
     9901 gcaggtcgtt gatgagcttg agcaggcgca tcgcattgcc gtgcatggtg gcgagcattt
     9961 catccatctc cgccgccgcc atcacgcgcc cgcggctcag cagggtctcc agcggggcga
    10021 tgagcagggt cagcggagtg cgcagctcgt ggctgatgtt ggcgaaaaac cggctcttga
    10081 gctcgtcgag ttcccgcagc ttctggttgg actcctcaag ctgggcgcgg ttgatgtcga
    10141 gacgccagcg cagctcgaac tcgctttgac ggatgctgtt gtaataccag cttccggcaa
    10201 taatgaagac gccggtgaca aagagaaagt agaggttgtt gaagtaaagt ccgtcaaatt
    10261 gaaacggctc gtgcatcacc gtggcgagga tgtaggccag gaaggtgagc aggaaaatga
    10321 tgacgctgtc cattgaggac cagcgcagca ggatggccga tccaagcatc acgaggttca
    10381 aacccgcgta atagggcgag gcggtgccct gctcggcgta aatcatccat gcgatggcca
    10441 gcagggggat gagagccacg agaaagccca gcgcgcggta atggcggcat tgcggaaact
    10501 tgatcagcgc gacgccaacg aaggcgagaa aggccgtggt aaggatgcgc gccggcgcga
    10561 aacggacgac gccctcccag ccgtaaacga agaaatcgag cacggcccct gccggcatga
    10621 aaacggccgc gagcaaggct cccagcttgt agttcctgat gcggatgggc cgctcgtatt
    10681 cgttgaacga acgctccaat tcctccacgg agactgtgct tgtctctggc atcaggcggg
    10741 tttgcggatt tcgaggaaaa tgttcacacc ggtcgagtcg gccttgatgt ccaccgaacc
    10801 gcgcggggcc ttctctggag ccagtttgag gaacgattcc ctgtcacgat agacaaggtt
    10861 ccattcaagg acgtactcca tccagttccg cgaggggttg gagctgtcca cattggtggc
    10921 gaccaccagg ccgccgggcg ccgtcatgtc ataaaaaatc tccagcaagc ggcggcagat
    10981 ccggtcggaa agatagtcga acaagcccgc gcaatacaca agatcatagc ctttctggcc
    11041 gggcgccggc ctgcccgcct ctttgaggat ttgatgcacc gatttctttt cgaatttcag
    11101 cccggtgcgc ctgcggtggc gcgcgcggat gtcctccagc acgcgggtgg tgttgaccag
    11161 ggtttcgtca ttgaagtcca gcagcaccat gtccgcctcg tcggagatgg agtcggtgtt
    11221 caggaagctc tggacttcct gggctgggcc gcaaccgagg ttgtggatgc gcgccacacg
    11281 cccctctccg gcggcgcgcg cggtctcgtc ccgaagccgc tcgatcaggt aggtgatgcg
    11341 gtttcggtgc gccacgaccg gggccgtgtc caggaaaacg cggttcagga gcttggcgaa
    11401 catcgagctg ccctcatacg ggtcgcgcag catcatgctg accatctcgt agtccccggc
    11461 gtatccgagc ggcttctgga aagtgcggta aatgaagggc gagcaaagaa cgagcgggtg
    11521 cagttgacgc ttgatgtaac tgcggtggat gggctgctgc tcgaccggaa tgacggaggc
    11581 gttgctttcg aagcgctcca tcaatggagc caccgccggc atgatgggga cttcaagttg
    11641 ctggatggtc tcccgttctg tggcgatgcg gtcgcctgtg ggcagcgagc gcacccccaa
    11701 ttcgacctgc tccatccagc gccgcaggtc cgtcagtagc gtctgcatgt cagcgacgac
    11761 cagtttgaac tcgggcgaaa cgcgataaat cctctccgtc tcgcgcagga agtccctgaa
    11821 ttcagacagc agcttttcct tgtccgccgt cggcgagacg aagtcaatcc agccctcctc
    11881 ggtcagcgag gcctcgcaga tcaacatgat gcccgtgttc accagattgg cgatcacggc
    11941 cctgccagaa taaaccacgc gctcattcat ggtgacccgg aagtcattca gcacttcgga
    12001 tagctggacg atgctgtagg ggttgtacac ctcgaaaacc gccatgtaac gagtgagtcg
    12061 gtgcagcgtg gcacggactt cggcaccctg gctgttgcgg cagacaacca tgctgatgcg
    12121 ctcggctgca tcggtgttca gcggcaaggg tgtgggcgcg ttttgctcca taagcattcc
    12181 agtaagttga gtggattgag tattgcaagc gacctttgcc caagagaatg aataatttgg
    12241 cggatttccc tctcgtatta agagtccgta ttagtcgaat gtgttccaag caaggatttt
    12301 tcatgaaaca gctccatgtg cagtatcttc tcacgtttga gaaaacattt gttatatgcc
    12361 gaaataaccc cgacaattcc aatcgtcatg caactcatgc caaccaatga agcgcccgtc
    12421 atcggcgaac ccgtgcagtt tgaatccttg ggggaaacga tctgcgcgct ctttgtgtct
    12481 tccatccgcc cggtcggcca gcccgtcctg gtggtgagcc atggagcggg tgaattcaag
    12541 gaaaactacc tcgaactggc ctcctatctc gcagacaggg gcatcgccag cctgctgctg
    12601 gacatgcacg ggcatggagc cagcggcggc cgccctttcc atgtgagcat gcgcgagtgg
    12661 gtgtcggaca tctcggcggc gctggattat ttggaaaccc gcgtggatgt ggatccggag
    12721 cgcatcggag ctttgggact ttcctcgggc ggctcggcca tcctggaggc ggcggtctgc
    12781 gacagccggc tgaaggcatt gatcccgctt gatgccacag tgatgaacac tctcccggca
    12841 ggggtaagcc tgaccatgag gctgctgtcc ctcatcggct gggtcaaacg aatgctcaca
    12901 gggaaggacc tgcgcatttc catcatcaag ctgctcgacg aagtgccgat ggcttccgac
    12961 cctgcgatca atgcgcgctt gaaggtggat ccaggcaaac tgaaagcgtt catgaatttc
    13021 cccatgccag gtgctgcgga cgcctttttt gtggacacca tccgacgcgt tccagccatc
    13081 aaggcaccca cgctggtgat ttggggggcg gatgaccagc tcgatccggt tagcaccgcg
    13141 cacatccttt tccaggcgtt gtcttgtgag aaagggttgg agatcattga aggaaatggt
    13201 catgtggggc acttggatcg caatcgggtg cgtgtttttg aggttgccac agattggctt
    13261 ttaaaatatt tggcatgaca cggattgtgt gtttaccatg tgctgacaac agcctcgttc
    13321 catgcgcctc attctcggcc aagaagccaa aactctcgaa cctgcggaaa agtggaaact
    13381 gctttcaccg gcgcttcggc agcatggcag ccaggccatg tcctatgcct gcctgcaaca
    13441 gggcatggag tactttgtgc atgaactggg cttcatcgcc ttcatcacag tgaagcaccc
    13501 ggtcttcgct cgcacgccga gacgcatcgc tttgacggac cctgtctgcg cggaggagga
    13561 tatgcccgcc ctggtggcag cgtttctgga aagccatccc tcggccgttt tcagcgtggc
    13621 ctcggagttt gcggcggatg ccatgcgccg gctcggcttc aaggccaact gcgtcggcta
    13681 cgaaccagaa ctgccggtcc agacatacaa cacggcggga aactggaagg aactggacat
    13741 gatcaagcgc gcgcgcaatg aggcgcgtcg tgaaggcatc accatacgcg aggtggacat
    13801 cgccacggtg cccgaggagc agcttcaggc gctctcacaa aactggattc agggcaagaa
    13861 ggtcaatgac cgggaaattt ggatctacgc ccggcggccg gtgtaccagt cagagccgga
    13921 tgtgcgcaaa tttgtggcct tcgacagaaa tggcagggcg gccggctatg tgttttatga
    13981 cccgatgtac cgcgatggca gggtctttgg ttactccgcc aacacggtgc gctgcgacga
    14041 gcggcagttt ggacgcctgg ccaccgccat ccacatgacg gcgatggacg ttttccgccc
    14101 ggagggtgtg gatgtgctca atctctgcct ctgccccttc acccatctgc aactgggccg
    14161 ctaccgggat gacctggcga cagactggtt tttccaggtc agccggcgtt acggcggaga
    14221 aatctacaac ttcgacggac tctccttcca caaatccaaa tatcgcggac gcgagaagcc
    14281 catctacttt gtctccaaca gcgcgcttcc atccaacgac gtgtatctcg ccttcctcgc
    14341 agccgacatc gccaccggct acttctccac catgggtctg ctggcgcgcg gcatatggaa
    14401 ggctgtgcgc ggacgcaaag gacgcggggc gggcggccag tccggttaga aacgcagcgt
    14461 ggtctggaga gcgaagaccc acgcattcgg gatggcgccc gatccgccgg ggtgcatcac
    14521 atattgcagg ttcggtgaca gcgtccacca tgcggcgagc tgtagcttgt agctcagttc
    14581 aacaaccgcc tcgtaatcgt gcggctcggc gaattgaaag ccgagcacct tgttgatgtc
    14641 ctccacagcc cgctccagat cacggctcat gcgcatgtat gagccggcaa tgcccagtgt
    14701 gtcgtaatcg cggccgggga tcaggccttt gaaaaccagg ccgccgtcaa tgctgtattc
    14761 agcgatgttc cggtccctcg gcgcccaggc ggtgcggaaa aagcccgtca ctccctgctg
    14821 cgccggatca tccggcccct gctcccgcca gagcatccgc tcggccagca aatacaggcc
    14881 gtaattgccg tcatgcgtgg tcggaccgtt gctaagaccg agggggatga gggaagcgat
    14941 cgtgttgtag atgtcgtaaa actcgtccgt gtggtagtag ccgcccgctt tcacgctcgt
    15001 cggcaggccc ttggccccct gctccatgtt gcgccgccag cccagctcat agaaaaacag
    15061 cgcaccctcg tcactgtgaa gctggatgcg cgtgccgggt cccgtcttgt caggatagcc
    15121 gtcataaacg cccgctttca aataaagcga ctgcgaggcg tcccatttca ataccaccgc
    15181 aggcaccccg taaggagtcg agggcagtga atgcgcccct ttgggaaaga atggctggtc
    15241 atagaggttg aacgccatcg tcgggaaaaa gaaggtctgg ttcaggaagt tgatggacgc
    15301 cagagagttg tacatctccg gcaaaatgaa atcccggtcc acgctcagca aacccgcctt
    15361 caaagtcagc ttctcgtcga gcagatgctg ctggaaatac acctgccaca cacggaagtc
    15421 cgccggaaaa tccaccaggt tggttttgtt caggtccccc acatactggt cggagaacgg
    15481 ctgcccgttg agccagacac tcgaagcgtg gaagctgccg cctttccaca gccccatttt
    15541 ctcaaaatcc aggtccaacg ccaggatcat cccgctttga taaatggcgc catggcggat
    15601 gccgccggaa atgttgttgg gcactgaacc cacataaaag aactccaggt ccacgccatg
    15661 atcggaaaga tcggaacgca gcccgcccca gtcccccagc atgtaatcct gcatgaacca
    15721 gcgttcgatg ggcgagccgc cggagggttc cgcattctcg gttgcgcaca gaggcgcaac
    15781 aacaaaggcg aagaaaaaaa caaaattgag tgagagggaa ggattcataa atgagaaaac
    15841 ttctaacaag ttatagaacg ttatgaaaat tcacaaattc tatttttgaa aagtgaaatt
    15901 cttgtcttaa ttaataaact taatacccaa agtttcaact tcactctgcc gctctcgctg
    15961 tttccacgcc cggcttgccc ctccccccct tttgtctatg aatgaccggg ccgatgcccg
    16021 actccttcgt ccacctccac gtccactccg acttctccct gctcgacggt gcctgccgca
    16081 tcagcgacct ggtcaaacgg gcgggaaaac tgggcatgcc cgctgtggcc ctcaccgatc
    16141 atggcaacct ctatggagcc atagacttct acatggccgc caaaaaggcc aaattaaaag
    16201 ccatccttgg ctgcgaggtc tatctggcgc ccggttccat gcgtgagcac aaagacgtgc
    16261 caggccgcca gcgcgcctcc catttgactc tcctcgccga aaacctcgcc ggctacgaaa
    16321 acctctcccg cctcgtcacc aaatcccacc tcgacggcct ctggctcgac accccgcgca
    16381 ttgacaagga cacgctgcgc gagcaccgcg agggcatcat ctgcctcagt ggctgcatga
    16441 gcggcgagat caacgagctc ctccactccg gccgccccga agaggccgag cacagcctgc
    16501 gcgagttccg cgacatcttc ggaccggacc attttttcct cgaattcatg gaccacggcc
    16561 tggagcagca gcagcatagc acgcgccagc tcgtcgagtg gggaaaaaaa tacggcctca
    16621 aaaccgtggc caccaacgac gtgcatttcc tccgccgaga ggaccacgag agccatgaca
    16681 ttctcatccg catcggcctc ggcgccaccg tccacgacac caacctccgc acctactccc
    16741 ctgaagtcca tttcaagacc gccgaggaaa tgcgcgccct cttccacgag ctgccggagg
    16801 cctgcgacac caccctcgac atcgccgacc gctgcaacct cgacatccgc ctcgactcca
    16861 ccagcattga ccgctacccc acctaccacc cgcccgacgg cagtgaccgc aatgactacc
    16921 ttcgccgcct ctgcgaggag ggcctcattc gccgttacgg gcgcgaccgc gccctgaacg
    16981 atgagaccct ccgccagcgc ctcgaaaaag agctctccct cctcagcgaa aaaaacttca
    17041 ccagctactt cctcatcgtc tgggacttca tcaactgggc gcgcgagcac ggcatccccg
    17101 tcggccccgg ccgcggctcc gcggccggct ccgtcgtcgc ctacgtcctc ggcatcacca
    17161 atcttgaccc ccttcgtttc gagctggtct tcgagcgctt cctgaacccc gagcgcgtca
    17221 gcccgccgga catagacata gatttctgcc agacacgccg gcctgaagtc atcgagtacg
    17281 tccgccagaa gtacggagag cgcagcgtca gccacatcat cacattcggc accatgggcg
    17341 ccaaaagcgt catccgcgat gtcggccgcg tcctcggctg gagctatggc gacgccgacc
    17401 gcctctccaa aatgatcccc accgagctga acatcacgct ccagggcgcg gaggaaaaag
    17461 acaaggaagg caaaaccagc ttcaaacccg gcgccattga caaaaaccca gaactcaaag
    17521 cctatgtcga aaccgacggc tccgcccagg aactctggcg gcacgccctt ttcctcgaag
    17581 gcctgacacg cggcaccggc atccatgccg ccggcatcgt cattggcgac aagcccctct
    17641 ccgagttcat cgccctcacc cgcggcaacg agggcgaaat cgtctcccaa tacgccatga
    17701 agcccctcac cgagctcggc atgctcaaga tggacttcct cggcctcaaa accctcaccg
    17761 tcatccacga ggccgagcac tgggtgcgca agcgcgtccc cggcttcgac accggccagg
    17821 tctccctcga cgacaccgcc accttcgaac tcctcaaacg cggtgacacc gtcgccgtct
    17881 tccaaatgga atccgggggc atggccgcca cctgcaaaaa cctcggcccc gacaccatcg
    17941 aggaaatcat cgccatcatc gccctctacc ggcccggccc catgcagttc attgacgact
    18001 acatcgcgcg caaaaaaggc ctccaaaaag tcgaataccc ccatcccctc ctcgaaccca
    18061 tcgccaaaga gacctacggc atcctcgtct atcaggagca ggtccagcag gccgcgaaca
    18121 tcctcgccgg cttctccctc gggcaggccg acatcctccg ccgcgccatg ggcaaaaaag
    18181 acgcccagga aatggcccgc cagcgtgtca tcttcgtcga aggcttcatc aagcaccgcg
    18241 gcgtacccga aaagcaggcc cacgccgtct tcgacctgct cgaaaaattc gcccaatacg
    18301 gcttcaacaa atcccactcc gccgcctacg gcctggtcac ctaccacacc gcctacctca
    18361 agaccaacta ccccgtcgag ttcatggccg ccgtgctcag ctatgaaatc agcaacaccg
    18421 acaaaatcgc cttcttcgtc agcgagtgcc agcgcatggg catcaccatc ctcccgcccg
    18481 acatcaacaa atcctccctc aaattcgccc ccgaaacctt cgcctccacc gagaccccgc
    18541 ctgccgtccc cggcatcccc aatgccatcc gctacggcct cagcggcatc aaaaacgtcg
    18601 gcgaggccgc catggccgcc gccatcgagg agcgccggaa aaacggcccc ttccacagcc
    18661 tcgacgactt cgcctcccgc atggacaccc gcgccgtgaa caaaagaatg ctcgaagccc
    18721 tcgtcaaatg cggcgccttc gacttcaccg gcgagccccg ccccgtcatg ttcgccaggc
    18781 tggaccctgt cctcgccgcc gcatccgccc tgcaaaaaga caggcagtcc ggccagggcg
    18841 gcctctttga cgacctcggc gccgcccccc cgcaaaaaac ccccgcgcgc cacaccgccg
    18901 tcgaaagctg gagccaggac gaaatcctcg ccttcgaaaa agagctcctc ggcttttacg
    18961 tcagcggcca cccgctcgac aaataccgcg gccacttcga ctcaccccgc atccaccgca
    19021 tcggcgccct cgacgaggtg gacaccgccg ccaaacccgt cagcctcttc atcctcggca
    19081 tcatcaagac cctcgacatc cgctactcca aaaaagacaa ccgccccttc tgcaccttca
    19141 ccatcgagga cttcagcggc cccgccgagg tcatggcctg gagcgacgaa ttcgaaaaat
    19201 tcaaagacct cctcaagccc ggcgccgtcc tcggcatgcg cgtccgctgc gccaaagacc
    19261 agcgcgggga tggcaaccgc gtcaccctct ccggcgacgt gcgcgaggcc aaacccaaga
    19321 aaccccgcga cccgaacgcc gccctccccg ccgcgcccgc cactcccgcc cctgccccgc
    19381 ccaagcccct cctcctgcgg ctcgactgcc gccgccacag ccagatagac ctcgaccgca
    19441 tccaggaagt cctcgccgcc caccccggca ccctccccgt catcttcgaa ttcctccgcc
    19501 gggacggcac ccgcctccgc ttccaggccg gcgacgaata ccgcgtcacc cagacccggg
    19561 agctcgtcca ggccctcatg atctggatgt gagcccccaa accaccgagc agcaggcagc
    19621 cctaccgggc ggaaaaagcc gtttcccagc tccgccaacc ccggggttgg cgtcgtgaat
    19681 ccggggattt ttggcaggga agtgccaggt cagcggtagc gcgaaaggcc atccagatac
    19741 tcccgcgcct gcgcagtggc gggctgggag aaaaagccgg cggcgctctg gctctcggcc
    19801 acttttcccg ccgccaggaa gaccacccag tccgccacct tcctcgcaaa gctgatctca
    19861 tgcgtggcaa gcacgatctg ctgcccgccc tcggccagct cgcggatgac atccagcacc
    19921 tcgcccgtca tcaccgggtc cagcgcggaa gtcggctcgt ccagcagcag caggcgcggc
    19981 cttggcgcca gcgcgcgggc aatggccgcc ctctgctgct ggccaccgga aagctccgcc
    20041 gggcgcttgc gcaaatgctc cgccaggccg agacgggcca
//
LOCUS       DAOMYJ010000082        20004 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Prosthecobacter sp. isolate AMR_MDS_5629
            NODE_937_length_20004_cov_21.854830, whole genome shotgun sequence.
ACCESSION   DAOMYJ010000082 DAOMYJ010000000
VERSION     DAOMYJ010000082.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867850
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prosthecobacter sp. (wastewater metagenome)
  ORGANISM  Prosthecobacter sp.
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Verrucomicrobiaceae; Prosthecobacter.
REFERENCE   1  (bases 1 to 20004)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 20004)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.30
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:34:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,742
            CDSs (total)                      :: 4,684
            Genes (coding)                    :: 4,676
            CDSs (with protein)               :: 4,676
            Genes (RNA)                       :: 58
            tRNAs                             :: 55
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20004
                     /organism="Prosthecobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_937_length_20004_cov_21.854830"
                     /isolate="AMR_MDS_5629"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1965333"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1793
                     /locus_tag="PLP58_10375"
     CDS             <1..1793
                     /locus_tag="PLP58_10375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ08967.1"
                     /translation="VIFENRLAGRIISPFFSAISGSSVARGHEGGMGLNAHKRLLLGR
                     YDIASGTFTEVPVPDKGRPTPAPDPSPGFAVNLPSVAVRGGQVWLGYRYFANTLWRAA
                     VTRFDPASGRWAEPAALPESTMGQDRRQELHTGPRGGVWITWATDQRTSKASGIAEIH
                     TAQLRHDADWPLLPEEPDAQVARAPLPAPYLNEPTPPRPLEEHHVWEIGGKTYRLVWG
                     DVHRHTDISNCRTGFDGCAVEHYRYALDLAGLDFLGTSDHTDIGKIYSPYEWWHTQRL
                     VDVFHAPGAFASLYAYEREQPFPWGHRNVVFSRRGGPVVYINRATYRASPWQAALPVA
                     AGIQPILPQELWDILRRHGQPCAIVSHTGATGMGTDWTQYEQGIDNLHENAVEIFQGA
                     RVSYEGPGLPQPTVGLRQGENYTPHRLAGDEHPKPPGAITDFDAGFPKAPGYNNGVYQ
                     KALAVGHKLGVFASSDHISTHTSFGGVYVEKFTREGILAGLNARRTCAATDKIFVELS
                     CGDRPMGAVFEMQGQPELRLRIEGTAPVRRVTVVRNEQDHHVLEPGARSVQTVWKDAA
                     PVPGENRYYLRIEQADGNMAWSSPLWITVR"
     gene            complement(1899..2126)
                     /locus_tag="PLP58_10380"
     CDS             complement(1899..2126)
                     /locus_tag="PLP58_10380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ08968.1"
                     /translation="MPFDPALPATGSALDSAVMRGQLTGLKDLIDAVPAVTGAVVDSV
                     TPLPPGDSPTASVALIDGVLHFSFGLPASGG"
     gene            complement(2152..3060)
                     /locus_tag="PLP58_10385"
     CDS             complement(2152..3060)
                     /locus_tag="PLP58_10385"
                     /inference="COORDINATES: protein motif:HMM:NF014108.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ08969.1"
                     /translation="MNTRFLGLLALVWVPGVLQAAVRSSTAYSTSIEALDAGGGTASS
                     AGYTQSVTSIGLIGWVQTSADYNSQTGYVPQTSSAAIGPTGGTMVLAPASPLDASAAL
                     TVTFSGWHDDDPPLSYAVLINNVVVSPQGNSALRNFTGPGAPGAYTLKGRIYDALGGF
                     TEVAQDFTVNTAQQSWRQQYFGTTANSGDAADGFDFDEDGLVNLLEWAAGLNPTLAST
                     LTTTAARSGELIEFHYTRSLTAAAAGAVFIVEWSDTLAANDWHTAGVTQQPLGDNGTL
                     QQMQATLPAGSLGRRFVRLRVTAPPP"
     gene            complement(3128..5020)
                     /locus_tag="PLP58_10390"
     CDS             complement(3128..5020)
                     /locus_tag="PLP58_10390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007414318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 28-related protein"
                     /protein_id="HRJ08970.1"
                     /translation="MKPTTLLFAAALLFSAVLPLAAQVPQIINYQGRISSGGTPFSGT
                     GQFKLALVNGAGNTTYWSNDGTSTAGSEPTAAVALPVVNGLYLVPMGDATLPNMTAIP
                     ATVFSNADVRVRVWFNDGVTGSQQLSPDQRITSVGYAMMAGSLPGVTATGTRVGIGTG
                     NPQAELDVSGAIRLGNGTTPAVAGMMRNGSGGLEGHDGTRWRSLLHHQLSVKEFGAKG
                     DGVTDDTAALQAAIDYSSSTITQVMVPKGTYMVTNLIIKSQTYLTGAHRGGTILRALP
                     SASPGLLTIATGPVVRVHLEDLRLSGGATGPVNVGQWAIYAESVPIATAPFHGGMWDS
                     VFEKVLVTNFDNGFWFKGGDSNTLLPHQQLKFDMVRIDRTNGSGISFKTTGQCEHFKH
                     IGCMYQAVGGTTTGTNVQLLKETTDGAGPFCQVFLDCTFQNTDKAVECLRAENLTFLN
                     CWFENVARAITVGTMSRAISVQNCRFANAADGGGSGYVLVSDNSRCSFRDNNISGTTD
                     KVITAISPESYILSEGNSVVSTLSTLTSGMTKQVAVAGGGELTLNEGNTFLVNSSATP
                     VSLINSKKLPGESVFIRAHGGQIQLATGGNISLGSAASPLTVPQNQVLQLVRYDLGGT
                     WIVVGK"
     gene            complement(5437..5754)
                     /locus_tag="PLP58_10395"
     CDS             complement(5437..5754)
                     /locus_tag="PLP58_10395"
                     /inference="COORDINATES: protein motif:HMM:NF026084.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4469 domain-containing protein"
                     /protein_id="HRJ08971.1"
                     /translation="MSSITPVIDNILDFITGQQDKYTPNNMIQLRGDDLRFKLSDTTQ
                     GVFFRSGSDPEVRASMYGQNEPGVVTAAIPAALSGPLQVRIAAYINGSVRSYTYTHLI
                     TAV"
     gene            5891..7864
                     /locus_tag="PLP58_10400"
     CDS             5891..7864
                     /locus_tag="PLP58_10400"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional serine/threonine-protein
                     kinase/formylglycine-generating enzyme family protein"
                     /protein_id="HRJ08972.1"
                     /translation="MTLPLPRPAPKAAEVTGAEAEALLAAGAGLFDTRPASSSPRGWE
                     PPGVELLQAVLPQYEITALIARGGMGAVYRGTQRALKRPVAIKVLPPEMNDAGGSDLQ
                     FAARFKQEAQAMARLSHPNIVAVFDAGEVVLGRALGPPSAAGGEERRTEDSPPHQTLL
                     YFVMEFIKGTDVAQLIASEGPLDAARVVPIIAAVCEALAFAHEEGIVHRDIKPSNIMI
                     DRKGRVKVADFGLAKAVNVESTLMTRSDVAMGTPDFVAPEALIPGMAVDGRADLYAVG
                     VMLYQMLTGKIPRGRFELPSGVVPQVDKGFDAIVDKAMQADREKRYSTALEMKREVEA
                     VAAPAKAWSGDAPVATPDHRLAPAATKGRKPLLLGTAAAVILGTGAFFLMDEPTEAES
                     KAGATTAGASPLTAASKDAPFVNSLGMKFVPVPIIGGPTGGKRVLFSIWETRVKDYEA
                     FAASSKRQWDRSHELPQGAEYPAIDVSWHDAVAFCEWLTAKEQKAGLIGSQARYRLPS
                     DHEWSCAAGNEYEDAAQLPSEKTIKRPIYPWGVAWPPPEGENLRGEENAGQKAPSWLE
                     IIPAYRDPYPLTAPVGLSKPSEHGLFDLGGNAGEWCADWYDASQKERVARGGSFNAGK
                     RLLCQSDYRTHFAPETHASSSGFRVVLDEAEAR"
     gene            7962..8501
                     /locus_tag="PLP58_10405"
     CDS             7962..8501
                     /locus_tag="PLP58_10405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ08973.1"
                     /translation="MTQNTLAHRLGTTTHVSPLLMKARRLGLRVPEDLCTLAVQRGCR
                     HYWQGDEPATPLLTQEQMSDEELAMALLCIAGQYDPHAIRCGAAMLGAEGNDPVHIAR
                     LAVWERSEAVVRYVAECGAKFEPENAFWSKLLKLMPQTPAPKPGVLPHPTRFVAMTGI
                     TRRGRETVMQWVRPGQSAA"
     gene            8543..9256
                     /locus_tag="PLP58_10410"
     CDS             8543..9256
                     /locus_tag="PLP58_10410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor"
                     /protein_id="HRJ08974.1"
                     /translation="MPTTHWSLIARLKGADSREARAALDDLCRAYHYPLYCQIRRHGL
                     SHHDAEDVLQEFLLKLLRLDTFGIADAEKGRLRTFLLVALRRFLSNWRRECWRRQERE
                     VSRECLEHLAGVEQRFQLDAEAHRESPDRLYDRQWARELMSRVVERLRVLYERKGREA
                     VFEALRPGLLQGGSLAGLESGQTALSLGLSAGALRTSFNRLLEDYREALRAEILQTVE
                     SRELAKAEYEELAALFSQG"
     gene            complement(9261..9902)
                     /locus_tag="PLP58_10415"
     CDS             complement(9261..9902)
                     /locus_tag="PLP58_10415"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF013827.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HRJ08975.1"
                     /translation="MIPLYDDNPVHRFPLVTILLIAANLAVYFLWQARVGLEESVALA
                     AFVPAEFTGGARPAAWRDLLTSMFMHGGIMHLVGNLWFLWIFGDNIENECGPVRFLVF
                     YLVCGVAATLAHVWQDPASAVPLVGASGAISGVLGAYLVQHPHAPVRTLIPLGMWSRV
                     IDVPAYVFLFIWIGMQVLSQAAASGGGGVAYLAHIGGFVAGGLLILIFRRRRA"
     gene            complement(9971..11008)
                     /locus_tag="PLP58_10420"
     CDS             complement(9971..11008)
                     /locus_tag="PLP58_10420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009962846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine synthase"
                     /protein_id="HRJ08976.1"
                     /translation="MDTRPLRTSVIGSYPFPGWLEFATRHLGQFGAADIAEMQDDAAA
                     IAIQDQLAAGLDVITDGEQTRFDFNLSFYGFIEGISLETASPRRFGPPAHDQRGRHEI
                     TGTLAAPRGLGVVEEWQRLKKLAAHSGARLKVSVPGPYTLSGRLAPNAQYPDRHAITE
                     ALLPLVRAEIESLVAAGAPEICVDEPSMSCYGHREDPRRFVDIFNRTVEPARGRARLC
                     THLCFGNYKGRAVGLRRYAPLFPAFHELHCDEMHVEMASREYAELEIIGAIARHTDAA
                     VGIIDVKSYHIETPEQVAEAVRACLRHAPAQRLVFAPDCGLSQTARWAARRKLENMVA
                     GVGIVRRELGL"
     gene            complement(11104..11778)
                     /locus_tag="PLP58_10425"
     CDS             complement(11104..11778)
                     /locus_tag="PLP58_10425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018971134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HRJ08977.1"
                     /translation="MEDPLPKEVTEDLVKEASGTLPTRKTLIERLDNWSDWSSWDEFY
                     RTYSGFVFHVARKAGLSDDEANDVVQETFIGVAKNLQKKKFDTSLGSFKSFLLNQARW
                     RILDQFRRRKKQQSREANLYSDETDERRTAPIDRCADPNGMALEKLWDQEWQDKVMDL
                     ALRRVKALVSPRQYQIFSCYVLKGWSPERVKKELGVNAAQVYLAKHRVGRILKREAAK
                     LAAEDQ"
     gene            11947..12390
                     /locus_tag="PLP58_10430"
     CDS             11947..12390
                     /locus_tag="PLP58_10430"
                     /inference="COORDINATES: protein motif:HMM:NF014062.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HRJ08978.1"
                     /translation="MIAEPFVLWFLAGLALVLLEFAAPGVIIVFIGMGAWAASLTTWL
                     GWTPTLGGQMGVFSAASVVFLLGLRNLCKDWFMGMSKNGDARETDEEFLNKEARVVTA
                     ITPDQPGKVEFKGAHWNARGIGALKPGDSAVIVGREGLQLVVRPR"
     gene            12440..13384
                     /locus_tag="PLP58_10435"
     CDS             12440..13384
                     /locus_tag="PLP58_10435"
                     /inference="COORDINATES: protein motif:HMM:NF013324.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045121 - membrane raft [Evidence IEA];
                     GO_process: GO:0034982 - mitochondrial protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="stomatin-like protein"
                     /protein_id="HRJ08979.1"
                     /translation="MILEFSTLVGLGFAAIVVIAVLKGARVVPQRNAFVVERLGKYSR
                     TLDAGFHVLIPFLDSVVAKHSLKEQVIDVPSQMCITRDNIAIEIDGVLFLQVLDPAKA
                     TYGVTNYFMAASQLAQTTLRSEIGKMELDRTFEERDNINANIVDALDKACEPWGVKVM
                     RYEISNINPPQSVKDALEKQMRAERERRAQVAMSEGDREARINVAEGHKQEAIKQSEA
                     VKIRQINEAEGKAREIELLAQATAQGIALIAQAINTPGGKDAVSLRVAEQYVTEFGRL
                     AQHTNTMIIPSNLSDIGGVVAGLGTLLDRTRQAGAAQG"
     gene            complement(13406..14851)
                     /locus_tag="PLP58_10440"
     CDS             complement(13406..14851)
                     /locus_tag="PLP58_10440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009961543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1501 domain-containing protein"
                     /protein_id="HRJ08980.1"
                     /translation="MSTFPLDRRQFLSGAYGMGTIALATLLQEHGLLANPALAGAQRH
                     FDLTPKPAHGFGRAKAMISMFMQGGPSHIDLFEPKPELVKLDGKDFPGEVKYDDAAGA
                     SREVMGPQWKFKPHGRSGIEMSELVPHMGGIADEMTLIRSMHTGVNNHGQSIYALMNG
                     QIVGGRPTLGSWITYGLGSENQDLPAYVALTDPRGLPVLGVDNFSNGWLPSVYQGTVI
                     RPKEPRILNLDPPMSLKGEAQERYLNFVQRLNRDHADARPGETDLEARIQSFELAARM
                     QTAAKEAMDISQESEATKKLYGIDKPETQEFGTRCLLARRLVERGVRFVSIFTGNQTW
                     DHHSSILKSLPAACKQVDQPAAALVMDLKQRGLLDTTIVHWGGEMGRLPVIQNRAGAK
                     GRESVGRDHNTYGFSMWVAGGGFKAGHAHGATDEFGHHAVKDVVNHYDYHATVLHLLG
                     LDTKKLSYKRNGVEQVLVENPQARVVTELLA"
     gene            complement(14909..15619)
                     /locus_tag="PLP58_10445"
     CDS             complement(14909..15619)
                     /locus_tag="PLP58_10445"
                     /inference="COORDINATES: protein motif:HMM:NF018178.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1080 domain-containing protein"
                     /protein_id="HRJ08981.1"
                     /translation="MKIHLLSAALLTLSGLTACNKPAETAGAPNAAEGWEKWFVNDLG
                     DAQIVSGTATYTVENGVLTGTTTEGSPNTFLIKGPFKDFELQFEVKVDDELNSGVQVR
                     SKVAKEGDPVIEGSKSGKPPPPGRLYGPQCEIAINGTAGDFYDEARRGTWWSILTGTE
                     AVRTEEAKAAFKKGEWNRYRIVVKGDHYQSFVNEVKTADFKQPGDPEGHIGFQVHGIK
                     KETGPYKVQWREVKFKAL"
     gene            15813..16049
                     /locus_tag="PLP58_10450"
     CDS             15813..16049
                     /locus_tag="PLP58_10450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ08982.1"
                     /translation="MARPTNTFETIPMTIAVTPQIRMYLDDLVMRGSYGSSPAEAARI
                     LISEAIEWKISDKKLDLKKFILQDGEVVAVPLAA"
     gene            16061..16267
                     /locus_tag="PLP58_10455"
     CDS             16061..16267
                     /locus_tag="PLP58_10455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ08983.1"
                     /translation="MNTMQPDTLADVLLFHVMMSIETEDADRSRPEAWKSETKLEILA
                     REIVQCDPALARHLVNDGMLKLAA"
     gene            16390..17073
                     /locus_tag="PLP58_10460"
     CDS             16390..17073
                     /locus_tag="PLP58_10460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ08984.1"
                     /translation="MTRFSKLLPALILLGACSTVKKLTPDLSRIPAPPLPSFSGLKKI
                     ATILPGMPESDTASVEDPKVPFNSRQTLGYGHTLRLHVYEGSRSTKRIYNGVVMVDEK
                     GVVAFPGVGSARVGGLELPKAAEAVATAFRVGLRISRAVTVHILSVEDLPLVAITGDV
                     IKDEYIPAWEKMTVKQAVTVAGGRRLGSANRGVYLIREGHSRYFPSLEAADRAEPEPG
                     DIIHLSTDL"
     gene            17080..17568
                     /locus_tag="PLP58_10465"
     CDS             17080..17568
                     /locus_tag="PLP58_10465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ08985.1"
                     /translation="MIEHIGGNPDANPNPWSSLFKSIVILRVGAGLLLLKYHAWAALH
                     SAYDFLWEEQPWEWVNHFNGAGLPWPTLLAPASAIVLGAVALSWITGFLTRLFAFIFL
                     PCVGAFIIFAGEKGAIHVEAGWLYLLAAFTLLLFGSGAVSLDKLFHIGENWGASKKKK
                     GW"
     gene            17569..18120
                     /gene="ruvC"
                     /locus_tag="PLP58_10470"
     CDS             17569..18120
                     /gene="ruvC"
                     /locus_tag="PLP58_10470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018968831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008821 - crossover junction
                     endodeoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HRJ08986.1"
                     /translation="MPRSTASTVAAPPAASARLLALDPALRNTGWAVLEKNGRDLRAL
                     AWGVIANPPKLLHSGCLVAIREQLSDVIRVHQPAVCAIETTIFVQSYKTAIVLGTARA
                     ACLIAAAEHGLPIYEYAPREVKQAAVGRGAAQKDQVAFMIRSMLRLRETPPADAADAL
                     AVGIAHFQNAEAATVLQRESRRV"
     gene            18208..19680
                     /locus_tag="PLP58_10475"
     CDS             18208..19680
                     /locus_tag="PLP58_10475"
                     /inference="COORDINATES: protein motif:HMM:NF024753.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HRJ08987.1"
                     /translation="MRYSWTALLLLPLLVTGQESFDRLTHFSAPSPLAKDAVTTDWPR
                     FLGPSDDASTTEKPLLKDFTSGGPKLLWRVDTGEGYTSPAIAGGRLVIFHALEGRETV
                     ECLHPESGRRFWSFDYPISYQDRYGFGNGPRGSPVIAEGVVVVLGVTSMLHALDLKTG
                     RELWRRDLRKDYNVPQDFFGHGSSPLITLGKVIVQVGGKEEPFDSFEDKRERARKLAS
                     KGVSVAAFDLKTGSVVWKVEDEWGASYASPTLAEIHGKTKVLVFAGGESDPPTGGLMC
                     MDPETGALHDRFPWRADDYISATASNPVVIPGMNRVFVTTCYPKNRPLGGVMVEYDTD
                     FKAREVWRSSKIGVHWMTPVHHEGHIYAVDGERENNSRLVCVNAATGGEVWEKNLEWE
                     DAALAQAQGRSNPVRLSILRANLLRCGEDFLCLGETGSLHWLRMSAAGLEESARTQLF
                     YALNTWSMPVVSRGLLYVRQHERSLDRKSGPALLCYDLRG"
BASE COUNT         3506 a         6131 c         6777 g         3590 t
ORIGIN      
        1 cggtgatttt cgagaatcgc ctcgccgggc gcatcatctc ccccttcttc tcggccatct
       61 ccggctcctc ggtcgcgcgc ggccatgagg ggggcatggg cctgaatgcc cacaagcggc
      121 tgctgctcgg gcggtatgac atcgcctccg gcaccttcac cgaggtgccc gtgccggaca
      181 aggggcggcc cacgcccgcg ccggacccga gcccgggatt cgcggtgaat ctgccctccg
      241 tggcggtgcg cggcgggcag gtgtggctgg gctaccgtta ctttgccaac acgctctggc
      301 gcgcggcggt cacgcgcttt gacccggcct ccgggcgctg ggcggagcct gccgccctgc
      361 cggagagcac catgggccag gaccgccgcc aggagctgca caccgggccg aggggcgggg
      421 tgtggataac ctgggccacg gaccaacgca ccagcaaggc cagcggcatc gccgaaatcc
      481 acaccgccca gctccgccat gacgcggact ggcctttgct gccggaggag ccggacgcgc
      541 aggtggcgcg cgccccgctg cccgcgccct acctgaatga gccaacgccg ccccgcccgc
      601 tggaggagca ccatgtgtgg gagatcggcg gcaagaccta ccgcctggtg tggggggatg
      661 tccaccggca cacggacatc tcgaactgcc gcacgggctt cgatggctgc gcggtggagc
      721 attaccgcta cgcgctggac ctggcggggc tggatttcct gggcacctcg gaccatacag
      781 acatcggcaa gatttactcc ccctatgaat ggtggcacac gcagcgcctg gtggatgtgt
      841 tccacgcgcc gggagccttt gcctccctct acgcctacga gcgggagcag cccttcccct
      901 gggggcaccg gaatgtggtc ttttcccgcc gcggcggccc ggtggtgtac atcaaccgcg
      961 ccacctaccg cgcctcgccg tggcaggcgg cgctgccggt ggcggcgggc atccagccca
     1021 tcctgccgca ggagctgtgg gatatcctgc gccgtcacgg gcagccctgc gccatcgtca
     1081 gccacacggg tgccacgggc atgggcacgg actggacaca gtatgagcag ggcattgaca
     1141 acctgcatga gaacgcggtg gagatcttcc agggcgcgcg cgtcagctac gagggccccg
     1201 gcctgcccca gcccacggtg ggtctgcgcc agggggagaa ttacacgccg caccggctgg
     1261 ccggcgacga gcacccgaaa ccgccggggg ccatcaccga cttcgacgcg ggcttcccga
     1321 aggccccggg ttacaacaac ggcgtttatc aaaaggcgct ggccgtgggg cacaagctgg
     1381 gcgtctttgc cagcagcgac cacatctcca cccacacctc cttcggcgga gtgtatgtgg
     1441 agaagttcac gcgggagggc atcctggccg ggctgaacgc gcgccgcacc tgcgccgcca
     1501 cggacaagat cttcgtggaa ctgagctgcg gtgacaggcc gatgggggcg gtctttgaga
     1561 tgcagggcca gccggagctg cgcctgcgca tcgagggcac ggccccggtg cggcgggtga
     1621 cggtggtgcg caacgagcag gaccatcatg tgctggagcc cggcgcgcgt tccgtgcaga
     1681 ccgtgtggaa agacgccgcg ccagtgccgg gggaaaatcg ctactacctg cgcatcgagc
     1741 aggccgacgg gaacatggcc tggtcatccc cgctgtggat cacggtcagg tgaggatggc
     1801 cttggaggtc caaagcagat cacgcccgcc ggcggcggaa gatgaggatg agcaggccgc
     1861 cggcgacgaa gccgccggta tgcgcggcag ggcgggcctc agccgccgga ggcgggcagg
     1921 ccgaagctga agtggagcac gccgtcaatg agggccacgg aggcggtggg ggagtcccct
     1981 gggggcaggg gagtgacgct gtccaccacg gcgccggtga cggcgggcac ggcatctatg
     2041 aggtctttga ggccggtgag ctggccgcgc atgacggcgc tgtccagggc ggagccggtg
     2101 gcggggaggg cggggtcgaa gggcatggga gaagggagag agaaggagcc gtcacggcgg
     2161 gggcgcggtc acgcgcaggc gcacaaagcg tctgcccagg cttcctgcgg gcagcgtggc
     2221 ctgcatttgc tggagcgtgc cgttgtcgcc cagcggctgc tgggtcacgc ctgcggtgtg
     2281 ccagtcattg gcggccaaag tgtcgctcca ttccacgatg aagaccgccc cggctgccgc
     2341 tgccgtgagg ctgcgggtgt agtggaactc gatcagctct ccactcctgg ccgctgttgt
     2401 cgtcagagtg ctggccaggg tgggattgag cccggcggcc cattccagca ggttcaccaa
     2461 gccgtcctcg tcaaagtcaa agccatccgc cgcatcgccg ctgttggccg tggtgccgaa
     2521 atactgctgc cgccagcttt gctgcgccgt attcaccgta aagtcctgtg ccacctccgt
     2581 gaagccgcca agagcgtcat agatgcgccc tttcagcgtg taggcacccg gtgcccccgg
     2641 ccccgtgaag ttgcgcaagg cactgttgcc ctgcggggac accaccacat tgttgatcag
     2701 caccgcgtag ctcagcggcg ggtcgtcgtc atgccagccg gaaaacgtca ccgtcagcgc
     2761 ggcggaggca tccaacggag acgcgggtgc cagcaccatc gtgccgccgg tggggccgat
     2821 ggcggcgctg ctagtctggg gcacgtaacc agtctggctg ttatagtccg cgctggtctg
     2881 cacccagccg atcaagccga tgctggtgac ggattgcgtg tagcctgcgg agctggcagt
     2941 accgccgccg gcatccagcg cctcgatgct ggtggagtag gcggtgctgc tgcgcacagc
     3001 tgcttggagc acgcccggca cccaaaccaa ggccagcaaa ccgagaaatc gcgtgttcat
     3061 aggagagtaa gagatgcaag ccatgcgcaa caagtttggg atgcagcggc ggctcatgcg
     3121 tgactgctta tttgccaacc acaatccagg tgcctccaag gtcatagcgc accagttgaa
     3181 gcacctgatt ctgtggcaca gtcagaggtg aggcggcgga acccagggag atattgccac
     3241 ctgtcgccag ttgaatctgt cctccatgag ctctgataaa taccgattca cctggcagtt
     3301 ttttggagtt gatcaaactg acaggtgttg cgcttgaatt gacgagaaag gtgtttcctt
     3361 cgttgagggt aagttctccg ccgcccgcca ccgccacctg cttggtcatc ccagacgtca
     3421 gcgtggacag agtgcttaca acggagttcc cctcgcttaa aatataggat tccggtgata
     3481 tggctgtgat caccttgtcg gtggttccag aaatgttgtt gtcacggaat gagcagcgcg
     3541 aattgtcgga aacaagcaca taaccgctcc cgcctccatc cgccgcgttg gcaaagcgac
     3601 aattttgcac cgatatggcg cgtgacatcg tccccaccgt gatggctcgg gccacattct
     3661 caaaccagca gttgagaaac gtcaggttct ccgctctgag acactcaacc gctttgtcgg
     3721 tgttttgaaa ggtgcagtcc aggaacactt ggcaaaaggg gcccgcacca tcggtcgtct
     3781 ccttgaggag ctgcacgttg gtgcctgtcg tggtaccgcc taccgcctgg tacatgcagc
     3841 cgatatgctt gaagtgttcg cactgtcccg tggtcttgaa ggaaatacct gaaccattgg
     3901 ttctatcgat ccgcaccatg tcgaacttca attgttgatg tggcagcaag gtgttggaat
     3961 ctccgccttt gaaccagaac ccattgtcaa agttggtgac gaggaccttc tcaaacaccg
     4021 aatcccacat gccgccatga aacggcgctg tggcaatcgg cacggattcc gcataaatcg
     4081 cccattgccc cacattcaca ggcccagtgg ctccgccgga gagtcttaga tcttccagat
     4141 ggacacggac cacggggcca gtggcgatcg taagcaggcc tggtgatgca ctaggcaaag
     4201 cacgaagaat agtgccaccc cggtgggcgc cggtcagata agtttgcgac ttgatgatca
     4261 aattggtgac catgtaggtc cctttgggca ccatcacttg tgtgatggtg ctggatgaat
     4321 aatcaatcgc agcctgcaac gcagccgtgt catccgtcac cccatcgccc ttcgccccga
     4381 attctttgac gctcaattgg tggtgcaaaa gcgatctcca tcgtgttcca tcgtgtcctt
     4441 cgagaccacc actgccattc ctcatcatgc ccgcaactgc aggtgtggtg ccatttccca
     4501 gccggatcgc cccgctcaca tccagttcag cctgtggatt gcctgtgccg atccccacac
     4561 gcgtgccggt ggccgtgacc ccaggcaaag agcccgccat catcgcataa cccacggagg
     4621 tgatgcgctg gtccggggag agctgctggc ttccggtgac accatcattg aaccacaccc
     4681 gtacccgcac atccgcgttg ctgaagaccg tggcgggaat ggccgtcatg ttcggcagcg
     4741 tggcgtcccc catgggcaca aggtagaggc cgttgaccac gggtagagcc acggccgccg
     4801 ttggctcgct gcctgcggtg ctggtgccgt cattgctcca gtaggtggtg ttgccggcgc
     4861 cgtttaccag cgccagcttg aactgcccag tgccgctgaa aggcgtgccg ccggagctga
     4921 tgcgcccctg gtagttgatg atctgcggca cctgggcggc gaggggaagg acagcactga
     4981 aaagcagcgc ggcggcgaat aggagggtgg tgggcttcat gagtggggga gcaggagcgg
     5041 aatgacgaat cagagggcgg gggaggggcc gctggacccc gggggtgacg cggcgggtga
     5101 tggcacctgc gcgggctgga ccccggacct gccggagcgg gagatggcac ctgcgcgggc
     5161 cggaccccgg acctgccgga tcgggaggtg gcacctccac tgacgccacc ccggacctcc
     5221 ccgagccgag ggtggcacct gcgcgggccg gaccccggac ctccgggagc gggaggtgcc
     5281 atctcccgga gccggacccc ggacctgcgg cggctgcacc ccggacctcc ggctgccgga
     5341 ggtggcacct gtttgaaggc ttgagggtgg gggtgcatgg gcaaggacga agcgcgaatg
     5401 acgaatgacg aagggggagc ggtgctgcgc cctgcctcac acggcggtga tgaggtgcgt
     5461 gtaggtgtag ctgcggacgc tgccgttgat gtaggcggcg atgcgcacct ggagcgggcc
     5521 gctgagcgcg gcgggaatgg cagcggtgac gacaccgggt tcattttgcc catacatgct
     5581 ggcgcgcacc tcggggtcgg agccggagcg gaagaagacg ccctgggtgg tgtcgctcag
     5641 cttgaagcgc aggtcatccc cccggagctg gatcatgttg ttcggggtgt atttgtcctg
     5701 ctggccggtg atgaagtcga ggatgttatc aatgacgggg gtgatgctgc tcatgctcag
     5761 gtggcagtac ccagcgggtg gaaaatcgtt acggacttta ttttgcccaa gggaaacacc
     5821 cgccttgatg ttccggcacg aaagccgctt aatgaagcgt ttacctaccc gccgcatgga
     5881 tgaccctgat gtgaccctac ccctcccgag acccgcgccg aaggctgcgg aggtgacagg
     5941 cgctgaggcg gaggcgctgc tggccgcggg tgcggggctc tttgatacaa gaccggcctc
     6001 cagcagcccg cgtggctggg agccgccggg tgtggaactg ctgcaagcgg tgctgccgca
     6061 gtatgagatc acggcgttga tcgcgcgcgg cggcatgggg gcggtgtacc gcggcacgca
     6121 gcgggcgctg aagcggccgg tggccatcaa ggtgctgccg ccggagatga acgatgcggg
     6181 cgggagcgac ctgcagttcg ccgcccgctt caagcaggag gcgcaggcca tggcgcggct
     6241 ctcccacccg aacatcgtgg cggtgtttga tgccggggag gtggtgctgg gtagggcgct
     6301 tggtcctcca agcgccgccg ggggtgagga gcggcggact gaggacagtc cgccccacca
     6361 gacgctgctc tactttgtga tggagttcat caagggcacg gatgtggcgc agctcatcgc
     6421 cagcgagggg ccgctggacg cggcgcgcgt ggtgcccatc atcgccgcgg tgtgtgaggc
     6481 gctggccttt gcgcatgagg aggggatcgt acaccgggac atcaagccct ccaacatcat
     6541 gattgaccgc aagggccgcg tgaaggtggc ggacttcggc ctggcgaagg cggtgaatgt
     6601 ggagagcacg ctgatgacgc gcagcgatgt ggccatgggc acgccggact ttgtggcgcc
     6661 ggaggcgctg atccccggca tggcggtgga cggccgggcg gacctctacg cggtgggcgt
     6721 gatgctctac cagatgctga cggggaagat cccgcgcggg cgctttgagc tgcccagcgg
     6781 cgtggtgccg caggtggaca agggttttga cgccatcgtg gacaaggcga tgcaggcgga
     6841 ccgcgagaaa cgctactcga cggcgctgga gatgaaacgg gaggtggagg ccgtagccgc
     6901 gcctgcaaag gcgtggtcag gagacgcgcc ggttgccacc cctgaccacc ggcttgcgcc
     6961 ggcagctacg aaaggccgca aacccctcct cctcggcacc gccgccgccg tgatccttgg
     7021 cacgggtgcc ttctttctga tggatgagcc gacggaggcg gagagcaagg cgggcgcaac
     7081 gaccgccgga gccagccccc tgactgccgc cagcaaggac gcgcccttcg tcaacagcct
     7141 cggcatgaag ttcgtgcccg tgcccatcat cggcgggccg acgggcggca agcgcgtgct
     7201 cttcagcatc tgggagacgc gggtgaagga ctacgaggcg tttgcggcct cgtcgaagcg
     7261 tcagtgggac cgctcccatg aattgcctca aggtgctgaa tacccggcca ttgatgtgtc
     7321 ctggcatgat gcggtggcct tctgcgaatg gctgacggcc aaggagcaga aagcaggact
     7381 gatcggcagc caggcacgct atcgcctgcc cagcgatcat gaatggagct gtgccgcggg
     7441 caacgaatac gaggatgccg cccagctccc ctcggaaaag accatcaaac gtcctatcta
     7501 tccctggggc gtcgcgtggc cgccaccgga gggcgagaac ttgcgcggcg aggagaacgc
     7561 cggccaaaaa gcccccagct ggcttgagat catccccgcg tatcgggatc cttacccttt
     7621 gaccgcgccc gtcgggctct ccaagccgag cgaacatggg ctcttcgacc tcggcgggaa
     7681 tgcgggcgag tggtgcgcgg actggtatga cgcctcgcaa aaggagcggg tggctcgagg
     7741 gggcagtttc aacgctggca aacgtctatt gtgtcaatcc gactatcgca cccattttgc
     7801 gcccgaaacg cacgcctcct cctctggctt tcgcgtggtg ttggacgaag ccgaggcgcg
     7861 gtaggcggcg gggcttgagg aatgacgtag gcggagggag cacaggacac catgcgaaac
     7921 ctggaattgc caggcgcgtg cccagaactt atgctgaaat catgacgcag aacacgctgg
     7981 ctcaccggct gggcaccacg acccacgttt caccgctgct gatgaaggcg cggcggctgg
     8041 ggctgcgcgt gccggaggat ctctgcacgc tggcagtgca gcgcgggtgc cggcattact
     8101 ggcagggaga cgagcctgcc acgccgctgc tgacccaaga gcagatgtcg gacgaagagc
     8161 tggcgatggc actgctgtgc atcgcgggcc agtatgatcc gcacgcgatc cgctgcgggg
     8221 cggcgatgct gggtgcggag ggcaatgatc ccgtccacat cgcacgactg gccgtctggg
     8281 aacgcagtga ggcggtggtg cgctatgtgg ctgagtgtgg cgcgaagttt gagcccgaga
     8341 atgccttctg gagcaagctg ctcaaactca tgcctcagac acccgcccct aaacccggtg
     8401 tgctgccgca tccgacgcgc tttgtcgcca tgaccggcat cacacgacgc ggccgtgaga
     8461 cagtcatgca atgggtacgc cccggtcaaa gcgccgcctg acctgtctgt tcctcatgcc
     8521 tttctccgat cccagccccc agatgcccac gacgcattgg agcctgatcg cgcggctgaa
     8581 gggcgcggac agccgggagg cccgcgccgc gctggatgat ctctgccggg cctaccatta
     8641 cccgctctac tgccagatcc gccggcatgg actgtcccac cacgatgcgg aggacgtgct
     8701 gcaggaattc ctgctcaagc tgctgcggct ggacaccttt ggcatcgcgg atgcggagaa
     8761 ggggcggctg cgcaccttcc tgctggtggc gctgcggcgc tttctttcca actggcggcg
     8821 ggagtgctgg aggcggcagg aacgcgaggt gagccgggag tgcctggagc acctggcggg
     8881 ggtggagcag cgcttccagc tcgacgcgga ggcgcaccgc gaaagtccgg accgccttta
     8941 tgaccgccag tgggcgcggg agctgatgag ccgggtcgtg gaaaggctgc gtgtcctata
     9001 tgagcgcaag gggcgggagg ctgtctttga ggcgctacga ccgggcctgc tccagggcgg
     9061 cagcctggcc ggactggaaa gcgggcaaac ggcactgagc ctggggctga gcgccggggc
     9121 gctgcgcaca tcctttaacc ggctgctgga ggactaccgg gaggcgctgc gggcggaaat
     9181 cctgcagacg gtggaaagcc gggagctggc gaaggcggag tatgaggagc tggcggcctt
     9241 gttcagccag ggctgagcgc tcacgcccgc cggcggcgga agatgaggat gagcaggccg
     9301 ccggcgacga agccgccgat gtgcgccagg taggccacgc cgccgccgcc ggaggctgcg
     9361 gcctggctga gcacctgcat gccgatccag ataaagagga agacgtaggc gggcacgtca
     9421 atgacccggc tccacatgcc cagcgggatg agcgtgcgca cgggtgcgtg cgggtgctgc
     9481 acgaggtagg cgccgagcac gccgctgatg gcgccgctgg caccgacgag gggcacggct
     9541 gaggcggggt cctgccacac atgggcgagc gtggcggcga cgccgcagac gaggtagaag
     9601 acgaggaagc ggacggggcc gcactcgttt tcgatgttgt cgccgaagat ccagaggaac
     9661 cagaggtttc ccaccaggtg catgatgccg ccgtgcatga acatgctggt gaggaggtcc
     9721 cgccacgcgg cgggccgggc gccgcccgtg aactcggcgg ggacgaaggc ggctagggcg
     9781 acgctttcct ccaggccgac gcgcgcctgc cagaggaaat agacggcgag attcgcggcg
     9841 atgagcagga tggtgacgag cggaaagcgg tggacggggt tgtcgtcgta gaggggaatc
     9901 atgccgggag tgtggcgcgc ggcggtcggc gcgcaagaag gcgcggcgcc gggcatcgtc
     9961 atgacgacac tcagaggccg agctcgcggc ggacgatgcc gacgccggcg accatgttct
    10021 ccagtttccg ccgggcggcc cagcgggcgg tctggctgag gccgcagtcg ggggcgaaga
    10081 cgagacgctg cgcgggggcg tggcgcaggc aggcgcggac ggcttcggcc acctgctccg
    10141 gggtctcgat gtggtagctt ttcacgtcaa tgatgccgac ggcggcatcg gtgtgacggg
    10201 cgatggcgcc gatgatctcc agctccgcat actcgcggct agccatctcg acgtgcatct
    10261 cgtcgcaatg cagctcgtgg aaggcgggga agagcggggc gtagcggcgc aggccgacgg
    10321 cgcggccttt gtagttgcca aagcaaaggt gggtgcaaag gcgggcgcgg ccgcgggcgg
    10381 gctcgacagt gcggttgaag atgtccacaa agcggcgcgg gtcctcgcgg tggccgtagc
    10441 agctcatgct gggctcgtcc acgcagatct cgggcgcgcc ggcggcgaca aggctctcga
    10501 tctccgcgcg cacaagcggg agcagggcct cggtgatggc gtggcggtcg ggatattgag
    10561 cgttgggcgc gagcctgccg ctgagggtgt aggggccggg cacgctgacc ttgagccggg
    10621 cgccggagtg ggcggcgagc tttttgagcc gctgccattc ctccacgacg ccgagaccgc
    10681 gcggggcggc gagtgtgccg gtgatctcgt gccgcccgcg ctggtcatgg gcgggcgggc
    10741 cgaagcggcg ggggctggcg gtctccaggg agatgccctc gatgaagccg tagaaggaga
    10801 ggttgaagtc gaagcgggtc tgctcgccat cggtgatgac atcaagcccg gcggcgagct
    10861 ggtcctggat ggcgatggcg gcggcgtcgt cctgcatctc ggcgatgtcg gccgcgccga
    10921 actggccaag gtggcgggtg gcgaactcca gccagcctgg gaaggggtag ctgccgataa
    10981 cggaggtgcg caggggccgg gtgtccatgc gcgcagccta cgcggcgcgg gcggcgcggg
    11041 tcaatgcgcg gggcgcatag tgcaactcgc gcgccgggaa aggacagggc gccactggcg
    11101 gagtcactgg tcctcggcag cgagcttggc ggcctcgcgc ttgaggatgc ggccgacgcg
    11161 gtgcttggcc agatagacct gggcggcatt gacgccgagt tcctttttca cccgctcggg
    11221 actccagccc ttgagcacat agcaggagaa tatctggtat tggcgggggg agacgagtgc
    11281 tttgacgcgg cgcagggcaa ggtccatgac cttgtcctgc cactcctggt cccagagttt
    11341 ttcgagggcc atgccgttgg ggtcggcgca gcggtcaatg ggcgcggtgc ggcgctcgtc
    11401 ggtctcgtcg ctgtagagat tggcctcgcg gctttgctgc tttttgcggc ggcggaactg
    11461 gtcgagaatg cgccagcgcg cctggttgag gagaaaggat ttgaaggagc cgaggctggt
    11521 gtcgaatttc tttttctgga ggttcttggc aacgccgatg aaggtctcct gcacgacgtc
    11581 gttggcctcg tcatcactga ggccggcctt gcgggcgacg tggaagacaa agccggaata
    11641 ggtgcggtag aactcgtccc agctcgacca gtcggaccag ttgtcgaggc gttcgatgag
    11701 ggtcttgcgt gtgggcaggg tgccggaggc ctctttgacg aggtcttcgg tgacttcttt
    11761 tgggagtgga tcctccatgc ggcgattgta ggagagggtg cgcggctggc aacggcttaa
    11821 tttgagccgt gccgcacgcg ggatggcatg aggggtgggg gcggcttcgg cggggcgcgc
    11881 tggttccaat ttcgggggaa agttgtagtt gccggcggga agggaatccg ggagtctcgg
    11941 cgcgtcatga ttgccgaacc ttttgttctc tggtttctcg ccggcctggc gctggtgctc
    12001 ctggaattcg ccgcgccggg agtcatcatt gtctttatcg gcatgggggc gtgggccgcc
    12061 tccctgacaa cctggctggg atggacgccg acgctgggcg ggcagatggg ggtcttctcg
    12121 gcggcgtcgg tggtctttct gctggggctg cggaacctgt gcaaggactg gttcatgggc
    12181 atgtcgaaga acggtgacgc ccgtgaaacg gacgaggaat ttctcaacaa ggaggcgcgc
    12241 gtggtcacgg ccatcactcc cgaccagccg ggcaaggtcg agttcaaagg cgcccactgg
    12301 aacgcgcgcg gcatcggcgc gctgaagccc ggcgactccg cggtgatcgt ggggcgcgag
    12361 ggactgcaac tcgtcgtccg cccgcgctga cccgcgctgt ttttcccgtc tcatccaaac
    12421 ccaagcacac ccacccacca tgatcctcga attcagcacc ctcgtcggac tcggcttcgc
    12481 cgccatcgtc gtcatcgccg tgctcaaagg cgcccgcgtg gtgccccagc gcaacgcctt
    12541 cgtcgtggag cgcctcggca aatactcgcg caccctggac gcgggcttcc atgttctgat
    12601 cccctttctc gacagcgtgg tggcgaagca ctcgctcaag gagcaggtca tagacgtgcc
    12661 ctcgcagatg tgcatcacgc gggacaacat cgccatcgag atagacggcg tgcttttcct
    12721 gcaggtgctc gatccggcga aggcgaccta cggcgtcacc aactatttca tggccgcctc
    12781 gcagctcgcg cagaccacct tgcgttctga gattggtaaa atggagctgg accgcacctt
    12841 cgaggagagg gacaacatca acgccaacat cgtggacgcc ctggacaagg cgtgcgagcc
    12901 ctggggtgtg aaggtgatgc gctacgagat cagcaacatc aacccgccgc agtcggtgaa
    12961 ggacgcgctg gagaagcaga tgcgcgccga gcgcgagagg cgcgcgcagg tggcgatgtc
    13021 ggagggggac cgcgaggcgc gcatcaatgt ggccgaaggg cacaagcagg aggccatcaa
    13081 gcagtcggag gcggtgaaaa tccgccagat caatgaggcg gagggcaagg caagggagat
    13141 tgagctgctg gcccaggcaa cggcgcaggg catcgccctc atcgcccagg cgatcaacac
    13201 ccccggcggc aaggatgcgg tgagcctgcg cgtggccgag cagtatgtga ccgagtttgg
    13261 ccgcctggcg cagcacacca acacgatgat catcccctcg aacttgagtg acatcggcgg
    13321 tgtggtggcc ggtctcggca ccttgctcga ccgcacccgc caggcaggcg cggcgcaggg
    13381 ttgagatccg gcagggggcg ggtgctcaag ccagcagttc ggtgacgact cgggcctgcg
    13441 ggttttccac gagcacctgc tccacgccgt tgcgcttgta gctgagcttt ttggtgtcga
    13501 gacccagcag gtgcaggacg gtggcgtggt agtcgtagtg gttgaccacg tccttcacgg
    13561 catggtggcc gaactcgtcc gtggcgccgt gcgcgtggcc ggctttgaag ccgccgcccg
    13621 ccacccacat gctgaagccg taggtgttgt ggtcgcgccc gaccgattcc cggcccttcg
    13681 caccggcgcg gttttggatg acgggaagac ggcccatctc gccgccccag tggacgatgg
    13741 tggtgtcgag cagtccgcgt tgtttgaggt ccatgaccag cgccgccgca ggctggtcca
    13801 cctgcttgca ggccgcgggc aggcttttga ggatgctgct gtggtggtcc cacgtctggt
    13861 tgccggtgaa gatggagaca aaacgcacgc cgcgctcgac aaggcggcgg gccagcaggc
    13921 agcgtgtgcc gaactcctgc gtctccggct tgtcaatgcc gtagagtttt ttggtggcct
    13981 cgctttcctg gctgatgtcc atggcctcct ttgcggcggt ctgcatgcgc gcggccagtt
    14041 cgaagctctg gatgcgcgcc tcaagatcgg tctcaccggg gcgggcgtcg gcgtggtcgc
    14101 ggttcaggcg ctggacgaaa ttcaagtagc gctcctgcgc ctcgcccttg agggacatgg
    14161 gaggatcgag attcaaaatg cgcggctcct tgggccggat gacggtgccc tggtaaaccg
    14221 agggcagcca gccgttgctg aaattgtcca cgcccagcac cggcagcccg cgcgggtcgg
    14281 tgagcgccac ataagcgggg aggtcctggt tttccgaacc gaggccgtag gtgatccagc
    14341 tccccagggt gggcctgccg ccgacgatct ggccgttcat cagcgcgtaa atggactggc
    14401 cgtggttgtt gaccccggtg tgcattgagc ggatgagcgt catttcatcc gcgatgcctc
    14461 ccatgtgagg caccagctcg ctcatttcga tgccgctccg cccgtgaggt ttgaacttcc
    14521 actgcggtcc catgacttcc cggctcgcac cggctgcgtc atcgtatttc acctcgccag
    14581 ggaaatcctt gccgtccagt ttcaccagtt ccggctttgg ctcgaagagg tcaatgtggc
    14641 tgggacctcc ctgcatgaac atggagatca tcgccttggc gcggccaaag ccgtgagccg
    14701 gcttcggagt caggtcaaag tgccgctgcg ctcccgccag agcggggttg gcaaggaggc
    14761 catgctcctg gagcagggta gccagggcga tcgtgcccat gccataggca ccggaaagaa
    14821 attggcggcg gtcgagggga aatgtgctca cgatgcgcca gactacgccg cgcgacacgg
    14881 cgatctagcg ggccgggaac aaggaagctc agagcgcttt gaacttcacc tcgcgccact
    14941 gcaccttgta ggggccggtt tcctttttga tgccatgcac ctggaaaccg atgtggcctt
    15001 cggggtcgcc gggctgcttg aagtcggcgg tcttaacttc gttgacgaag ctctggtagt
    15061 ggtcgccctt caccacgatg cggtagcggt tccactcgcc tttcttgaaa gccgccttcg
    15121 cttcttcggt gcgcaccgcc tccgtgccgg tgagaatgct ccaccaggtg ccgcgtcggg
    15181 cctcgtcata aaaatcaccc gccgtgccgt tgatggcgat ctcacactgc gggccgtaaa
    15241 gacgtccggg aggcggcggc ttgccgctct tgctgccctc gatcaccggg tcgccctctt
    15301 tggccacttt gctgcgcacc tgcacgccgg agttcaactc gtcgtccacc ttcacttcga
    15361 actgcagttc gaaatccttg aacggaccct tgatcaaaaa ggtgttcggg ctgccctcgg
    15421 tggtggtgcc ggtgagcacg ccgttttcca cggtgtaagt cgccgtgccg ctcacgatct
    15481 gggcgtcgcc gagatcgttg acgaaccatt tttcccagcc ttcagcggcg tttggcgcgc
    15541 ctgcggtttc agcaggcttg ttgcaggcgg tgagtccgga gagtgtgagc agggcggcgg
    15601 agagcagatg gatcttcatg gtggcaggcg ggcatacaaa gtcgggaaac agggcaccgc
    15661 atcctgaaaa tctccctttt atttaagtta agttaaatac ctggaaatta taacgtcatt
    15721 attaaccgat aaaaaccact tcgcgcttgc gggaagtggt ggtttgcatt ttaatcacat
    15781 ttaaatccgg ttttaatttt caactcccgt caatggctcg ccccaccaac acttttgaga
    15841 ccatccccat gacgatcgcg gtcactccgc agatcaggat gtatcttgat gatctggtga
    15901 tgcggggatc atacggcagc agccccgctg aagcggcccg catcctcatt agcgaggcca
    15961 ttgagtggaa gatctcggac aagaaactgg atctgaaaaa attcatactt caagacggtg
    16021 aagtcgtcgc cgttccgctg gccgcctaac ctcgcaccct atgaacacaa tgcaacccga
    16081 cactctggcc gatgtcttgc ttttccatgt gatgatgagc atcgaaaccg aggatgccga
    16141 ccgctcccgc ccggaggcat ggaaatccga gaccaagctg gaaatactcg cacgggagat
    16201 cgtccaatgc gatcccgcgc ttgcccgtca tcttgtgaat gacggcatgc tcaaactggc
    16261 agcctgagag gcagtttctt tttgcgtcag tcaggcatgt ccagaatcaa aggccggcgt
    16321 ctttgacgtt ggccttgtca tttccaggca gggcgtccat catgcgaatc ctgattttcc
    16381 tttcgctcca tgacgcgctt ttcaaaactg ctgcccgccc tgattctgct cggggcttgc
    16441 tcgacggtca aaaaactcac tccggacctc agccgcatcc ccgcgccgcc gctgccatct
    16501 ttttccggtt tgaaaaagat cgccaccatc cttccgggaa tgccggagag cgacactgcc
    16561 agcgtcgaag atcccaaggt ccccttcaac tcccgccaga cgctcggcta cgggcacacc
    16621 ctgcgcctgc atgtgtatga aggctcccgc tccaccaagc gcatctacaa cggtgttgtg
    16681 atggtggatg aaaagggtgt cgttgctttc ccgggagtcg gctccgcccg tgtcggcggg
    16741 cttgagcttc ccaaggccgc cgaggccgta gccaccgctt tccgcgtcgg cctccgcatc
    16801 agccgggcgg tcaccgtcca cattctctcc gtcgaggacc tccctctcgt cgccatcacg
    16861 ggcgatgtca tcaaggacga atacatcccc gcctgggaaa aaatgaccgt caagcaggcc
    16921 gtcaccgtcg ccggcgggcg caggctgggc tctgccaatc gcggtgttta tcttattcgt
    16981 gagggccaca gccgctactt cccaagcctt gaagctgccg acagggccga gccggagccg
    17041 ggcgacatca tccacctttc cacagacctc tgaccagtca tgatcgaaca catcgggggc
    17101 aatcccgacg ccaacccaaa cccctggagc agccttttca aatccatcgt catcctccgc
    17161 gttggcgcgg gccttcttct cctcaaatac cacgcatggg cagcccttca ctccgcttat
    17221 gacttcctct gggaagagca gccctgggaa tgggtaaacc atttcaacgg tgccgggctg
    17281 ccctggccca ccctgctcgc ccccgcctcc gccatcgtgc ttggggctgt cgcgttgagt
    17341 tggatcaccg gcttcctaac ccgccttttc gccttcatct tcctgccctg cgtcggcgcg
    17401 ttcatcattt tcgccgggga aaaaggtgcc attcatgtcg aggccggctg gctttatctt
    17461 ctcgccgcct tcacgctgct actctttggc tccggcgccg tctccctcga caagctcttc
    17521 cacatcgggg aaaactgggg agcctcgaaa aagaagaaag gctggtaaat gccgcgctcc
    17581 accgccagca cggtcgccgc cccgccagcc gcatccgcgc gcctgctcgc gctggatccc
    17641 gctttgcgca acaccggctg ggccgtgctc gaaaaaaacg ggcgcgacct ccgcgccctc
    17701 gcctggggag tcatcgccaa cccgccgaag ctgctccact ccggatgcct cgtcgccatt
    17761 cgcgagcagc tcagcgatgt catccgcgtg catcagcccg cagtctgcgc cattgagacc
    17821 acgatctttg tccagagcta caagaccgcc atcgtcctgg gcaccgcccg cgccgcatgc
    17881 ctcatcgccg cggccgagca cggcctgccc atttatgaat acgcgccgcg cgaggtgaag
    17941 caggccgccg tcggccgcgg cgccgcgcag aaagaccagg tcgctttcat gatccgttcc
    18001 atgctccgcc tgcgcgagac tccgccagcc gatgccgccg acgcgcttgc agtcggcatc
    18061 gctcatttcc agaatgccga agctgccacc gtcctccagc gcgaatccag gcgggtgtga
    18121 tgaatcgcac cctggccgcc ctgcaccacg ggctgggcgg gcaagagcgc gcttgatgtt
    18181 ttccaacgcg gcctagcctt tgtgctcatg cgctattcat ggaccgctct gcttctcctg
    18241 ccgctgctcg tcaccgggca ggaatccttt gaccgtctga cccacttctc cgcgccctcg
    18301 cctttggcaa aggacgctgt gaccacggac tggcctcgtt ttctcggccc ctcggacgac
    18361 gcctcgacga cggaaaagcc gctgctgaaa gacttcacgt ccggcgggcc caagctgctc
    18421 tggcgggtgg acacaggaga gggttacact tcgcccgcca tcgcaggcgg tcggctggtg
    18481 atcttccacg cgctggaggg gcgtgagaca gtggaatgcc tgcatccgga aagtgggcgg
    18541 cgcttttgga gttttgatta tccgatcagc taccaggacc gctatggctt tggcaacggt
    18601 ccgcgcggaa gccccgtgat cgcggagggc gtggtggtgg tgctgggggt gacttcgatg
    18661 ctgcacgcgc tggacctgaa gacgggccgc gagctgtgga ggcgcgatct gcggaaagac
    18721 tacaatgtgc cgcaggactt ttttggtcat ggttcgtctc cgctcatcac gctaggcaaa
    18781 gtgatcgtcc aggtgggcgg aaaggaggaa cccttcgaca gcttcgagga caagcgcgag
    18841 cgcgcgcgca agctggcttc gaagggagta agcgtggcgg cctttgacct gaaaacgggc
    18901 tcggtggttt ggaaggtgga ggacgagtgg ggcgccagtt atgcctcgcc gaccctggcg
    18961 gagattcacg gcaaaaccaa ggtgctcgtt tttgccggag gcgagagtga tccaccgacc
    19021 ggcggactca tgtgcatgga tccggagacc ggcgcgctgc acgaccgctt cccctggcgc
    19081 gcggatgact acatctccgc cacggcatca aatccagtgg tcattccagg catgaaccgt
    19141 gtctttgtca ccacctgcta tccgaagaac cgcccgctcg gcggagtgat ggtggaatat
    19201 gacacggact tcaaggcgcg cgaggtgtgg cgatcgtcga agatcggtgt tcactggatg
    19261 acgccagtgc atcacgaggg gcacatctac gcggtggatg gcgagcggga aaacaacagc
    19321 cgtctcgtct gtgtgaatgc cgccactggc ggggaagtgt gggaaaaaaa tctggaatgg
    19381 gaggatgcgg cgctggcaca ggcgcagggc cggagcaatc cggtccgtct gagcatcctg
    19441 cgcgcgaatc tgctgcgctg cggggaggac tttctctgcc tcggtgagac tggcagcctg
    19501 cactggctga ggatgagcgc cgcaggattg gaggaatcgg cccggacaca gttgttttac
    19561 gcgctgaaca cctggtccat gccggtcgtc agccggggcc tgctttatgt gcgccagcac
    19621 gagcgcagtc tggacagaaa aagcggtccg gcgctgttgt gctacgactt gcgggggtga
    19681 cacatcaacg cccggcgggg agtttgcggc tggccgcatg cgccggggtc caggaattga
    19741 tgccgctcca cgcggaccaa tgtctccgcc atgtccagcc gcattcatgc atggtcgtgc
    19801 gcgtgatgag gttgtcccgc tcttttgcta tctttccggc aggcggaaat gatggctcga
    19861 cttgccgcgc tgccttggat agcttgcact catgcaaccg ccgcttctga cccgccgcac
    19921 cgccctccgc cgcactcttt ccaccacgct cagcgccagc gccgcggccg gctcaccggc
    19981 gaggctgggc tgcacgagca atgc
//
LOCUS       DAOMYJ010000083        19664 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Prosthecobacter sp. isolate AMR_MDS_5629
            NODE_959_length_19664_cov_21.320516, whole genome shotgun sequence.
ACCESSION   DAOMYJ010000083 DAOMYJ010000000
VERSION     DAOMYJ010000083.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867850
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prosthecobacter sp. (wastewater metagenome)
  ORGANISM  Prosthecobacter sp.
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Verrucomicrobiaceae; Prosthecobacter.
REFERENCE   1  (bases 1 to 19664)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 19664)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.30
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:34:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,742
            CDSs (total)                      :: 4,684
            Genes (coding)                    :: 4,676
            CDSs (with protein)               :: 4,676
            Genes (RNA)                       :: 58
            tRNAs                             :: 55
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19664
                     /organism="Prosthecobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_959_length_19664_cov_21.320516"
                     /isolate="AMR_MDS_5629"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1965333"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            197..1672
                     /locus_tag="PLP58_10480"
     CDS             197..1672
                     /locus_tag="PLP58_10480"
                     /inference="COORDINATES: protein motif:HMM:NF039513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5716 family protein"
                     /protein_id="HRJ08988.1"
                     /translation="MSQLLSQRLARSISADFFRPLARPSAPVYVDCADRLIDEAGEAG
                     RLSQRETIEIIKEVIARHPLSLLAEDEGAALRDARQRAGQFFNRLIAAGWLEDQTLGL
                     HERWAVITPGLRPLLRMLRDLAEDRVAELKTFADTLRGLCQTLEERGVLDPQIQPPDQ
                     LRSTITDLTQRLERAIEQLHAVEKIVTGFEQRQRQTATAADTLNLLYEEFGQGQHMVC
                     YDALRRGGLLTRIENARSAVSECHDDPLMRERLASGLREHYGYDDADAYDHAVAMLTR
                     LERDLGGLKRRAEAIDLRMAAFNRLSQQRYRYQTELRGRRPEIVKAYCDAINAAHAGG
                     RFSEFAGQGADFAPRCVETRFFFGTESLYKPRRTKSPVDLRFGETGASKQSEDDVLAA
                     WKERQRLALTPQRAARLVAKLLPAKGRTTSTEDITLETTDDLLDLLAAVAYEHAPALA
                     GKRVRWSVDGLRRRNGLEPHILEHDEIAGHRVERFAITREG"
     gene            1672..1995
                     /locus_tag="PLP58_10485"
     CDS             1672..1995
                     /locus_tag="PLP58_10485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ08989.1"
                     /translation="MPFDAWQMVATDYDQAMQFSQSPIELHPLMDLIRRDRVIKARRQ
                     TPEERFAEAMEMTDFAFEVMASSVKDSYPDADAQTATGILRKRLKRLRDREDRGIFVP
                     AGKQV"
     gene            2088..2549
                     /locus_tag="PLP58_10490"
     CDS             2088..2549
                     /locus_tag="PLP58_10490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ08990.1"
                     /translation="MIPRATKDIDLVIQVQEPQFTAFLKEVDSQFELDPQVYVETLTG
                     TKRYILEHRKTAFDVELFILSSDPHHLSMWSRKRLRRQVFCTRDAWTPTAEDMVIQKL
                     RWARPQDLVDADSMIVVQGDELDWPYIEHWCDLHGTRERLEKIRAEIPPME"
     gene            2633..3286
                     /locus_tag="PLP58_10495"
     CDS             2633..3286
                     /locus_tag="PLP58_10495"
                     /inference="COORDINATES: protein motif:HMM:NF025205.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4194 domain-containing protein"
                     /protein_id="HRJ08991.1"
                     /translation="MTSAPSDPLWPRSFWSDVPDSDRAALREVLAELLGRGVLLGDEG
                     GGRELYLLARDHYRTHVEDYLAPLALELIVDEERSLLQARPRTESCLLLGRFDKDETL
                     VLLALWRLYDDEISTGSQEAVIVTVDQLWAALKVYFDKISTPEKSQIEACLARIRRHR
                     LIRTLRPEGMTQAGEMQIEILPSLARVIPFDDIAAWQQRVEQYQPVKDSAEDGEAAA"
     gene            3283..6621
                     /locus_tag="PLP58_10500"
     CDS             3283..6621
                     /locus_tag="PLP58_10500"
                     /inference="COORDINATES: protein motif:HMM:NF024947.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SbcC/MukB-like Walker B domain-containing
                     protein"
                     /protein_id="HRJ08992.1"
                     /translation="MSAPRITLSRILAVNWYGYRQFIDVAGLSLITGANGSGKSALLD
                     LIQFVMLGEQQSKFNKAAAGAGSGRSLRGYCLCDTNTTGRDGAERYLRPSSVTLAALE
                     FTWPLQSGETELRRETWGARIEYESPTAKPSTIWFRAGRRLAWQDFLNSDAGPQAMQF
                     LPEDEFRTRVKRELDGDVWDRQQTYRDEMALRSHLGFDAEQMAKTLPRAMAFEPESNF
                     ERFVREFLLEPGMPDVKAVKASVDAHRRAQERLEKMYDQLERLKRISAHHQDWLTSKR
                     ESALYSHLSDALKHEEALEGLNRRRAELEDKRAEHAENLQAHEQAIRERERLEHAEES
                     ARAALGDKAFKLEEIDRRRRELDSEIERLNAASSSLHERIRAHLRRWQDWTLHASRLG
                     LQNLDEASAAISAMQSKDESKALAAARDTARAYMRLKDEANEQLQPVLARLSEHELRK
                     AALHKDLAQLREGHATPSPLLNALLSRGQKAVALGRVVEVKASAEKWWPLIETVLGIH
                     RRAVIPEDFRAAWDQAQQTQNPHEPLIHPEEAAQTTTKVLKGSLREMVETKHDLAGKV
                     LDHLLGGLVAVNKAAQLDKHERAISLDGWLKDPPRRVKLTPEKELTLGEEGLRRLRDL
                     RENELRETDAVIEEVRSDCDDLRAFLNRGAEGHLDRFTAPEGADEVQRLPKLREELRE
                     LQETWNLLATPDHEKAMENLRVQKATLKGIHERIGSLKTAITAFTLDQSRLEEAIQED
                     VFQEEQTRIARQASRMLVHGFSDEEITTRITAEHQKATSWRKSIEMAAALAIQLDTRA
                     QESRRLRDEQRRELITVHADLGDALDADAEDNAAFDQRREDLETHELERFKTAADEAR
                     KEWEDRLQHQVLDVLREKLSEADRTKRELNRAMDHEIGGWRYQLTSRADKAHSAIWTL
                     VEKGLPSGSEMELFNAAGREDIERAKAELMAAIDAADNPEDKRHQRMLDYRYYHHWDI
                     EAKPAGRGDAAAISLNKSAKKQSGGENQAPFFVATLAAFRRVYDLGRREEQQNLGLVV
                     MDEAFSKLSGDRIDDCLALARNFGLQLIMAFPEDRLPTMFQHADTVVQCRVERSYDES
                     TEQIQNIENWVVRVEGSRLAELME"
     gene            6809..8029
                     /locus_tag="PLP58_10505"
     CDS             6809..8029
                     /locus_tag="PLP58_10505"
                     /inference="COORDINATES: protein motif:HMM:NF021498.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2220 family protein"
                     /protein_id="HRJ08993.1"
                     /translation="MRWLQTLHEQWMTARKRRVEASVRAFRRDWETLLDDAGLTSAEE
                     RQAAQRDAEKLPLKLIPFRHKPRFIDKIEVPLESEAWLHAQFGSQTGAEAQQAALAVV
                     EQFSAKSHPLLPEAWTALLSRLKAEFALPHAVEPFRWREATRVEELLKLLFDLTSCEW
                     PEGTLIRDASTQIGRDSKYLEAQQSFIERALELLLGRETPLEALGIQTSNSVLHYSGP
                     LVLHFADHSKPLDLRFESTLSVAELERATHITTTAQRLLTVENRKTTFLQLTRADVTR
                     STLIVATSFPTQAVRLLLQKLPPDLPHHHFGDTDPAGWDILRKLREVSGRTVHPFHMH
                     WRPQANASPLTRRDYLILTRLFSNPSMADCRAAFEDMNAAGTKGDYEQESLGPPELEA
                     WPFYHALDRGGQPL"
     gene            8041..8817
                     /locus_tag="PLP58_10510"
     CDS             8041..8817
                     /locus_tag="PLP58_10510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012420677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase"
                     /protein_id="HRJ08994.1"
                     /translation="MNAKPLEGKTGLVFGVANKRSIAWAIAQAWAEAGAKLIFNYQGE
                     RLKDNVEELAGTFGTDTPLFPCDVSKDEEVDAFFDKVAAITPKVDLLLHSVAFAPKEA
                     LEGDFLSTSREAYLTAHNISAYSLVHLARKAAPLMTDGGSIIAMSYYGAEKVVPHYNV
                     MGVAKASLEASTRYLAYDLGKKKIRVNCISAGPVNTLAARGIAGFGSMLKHYDEHAPL
                     GRSCEPKELGATGVFLASEGAASITGQVIYVDGGYQIMGM"
     gene            complement(8953..10275)
                     /locus_tag="PLP58_10515"
     CDS             complement(8953..10275)
                     /locus_tag="PLP58_10515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018290394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HRJ08995.1"
                     /translation="MSTPDSKPAGISLSTAVAVVVANMVGTGVFTSLGFQVGGLPSGF
                     ALLVLWAVGGLCAFCGALAYGELAAAMPRSGGEYHFLARIFHPAAGFMAGWISFTVGF
                     AAPIALAAMAFGKYFGGVVPGASATVLSVFMVLVVAVVHSAGIHTAARFQNAATWMKV
                     ALILVFIGAGAALGEGHGARFTPAAEDWALVTSTPFAVSLVYVMYAYAGWNATTYIVG
                     DIREPRRNVPLSIALGTLLVTGLYLALNTVFLHAAPMDELEGRVEVGHVAAGHLFGES
                     GGRLMSGLICLGLVSSVSAMTWVGPRVAMTMGQDARLLAPLARVDRRGTPQVALALQT
                     LIVAALLMTSTFEKVLTCVQFSIQLCSFLTVLGVMVLRRTQPGLRRPVRCWGYPLTPL
                     IFMGISLWMLVYVLKERTWESLAGLGIMVSGLLVYAVSAKNGMTAKAL"
     gene            complement(10307..11734)
                     /locus_tag="PLP58_10520"
     CDS             complement(10307..11734)
                     /locus_tag="PLP58_10520"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HRJ08996.1"
                     /translation="MKHIFLLAALVLLPGFIEARPRNIVFMLLDNVGQEWFGCYGGEA
                     GVTPAIDKLAATGVRVEHCYTAPVCGPSRIVALTGRYPHSTGFRLHHDAALYSGGGLD
                     PEREILFPRLFQKAGFATCLAGKWQVNNLYDEPGVLDRHGFDEHLAWPGSLDKDKMTP
                     EQTAAFWDAVKRESVEDTVAFIQNIESRYWDPVFLRQGKRERHPGKFGTDVAREFAFD
                     FLRRHKDKPFMLYLPMVLTHGNAYPHPVTTTPLNREENRPHEEMFADMLRYADMQVDA
                     MVKELEKLGLRDDTILIVASDNGTEQSLSTRRSGRVVKGGLYKMNEAGGNVALIANNP
                     RHIPGGRVLPLADFTDLYPTFCELAGVPLDAARLPDGSSFARFLVDEKAPPHRDWILN
                     EYHTTRVVRDTRFKLYSEGPFFDANADPDEQHDLSSDSRPEVAAARAKLSAVLASLPP
                     DKPEPFPLRSQSGFKIRTELRAKRN"
     gene            complement(11771..12556)
                     /locus_tag="PLP58_10525"
     CDS             complement(11771..12556)
                     /locus_tag="PLP58_10525"
                     /EC_number="5.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047689 - aspartate racemase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid racemase"
                     /protein_id="HRJ08997.1"
                     /translation="MPARLILAALLFTSGISCSQLGMCKVAPKRMVGIFGGMGPEATA
                     NLYAEIVRLTPAKEDQDHLPTVIYSRPQVPDRTKCIESGSREIVPYIQEGVRRLERMG
                     ASLIAIPCNTVHYYHADMQKAVRVPVLNMITETVDAVEKRHPRCKVVGLLASNGTVRS
                     KLYEKAFEARGIRVVYPDAACQQTCVMNAVYSIKAGGDKRRQADLLARAADDVTRKGA
                     QVIVLGCTEIPLAFDSSRTRLPVVNATEVLALAAIREYQRGAP"
     gene            12600..13583
                     /locus_tag="PLP58_10530"
     CDS             12600..13583
                     /locus_tag="PLP58_10530"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009963446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HRJ08998.1"
                     /translation="MPGELSVDAGKSFCHATTRAEHAPVEWSIEPPDSGQRLDKHLAA
                     RLGDLSRARIQDLIREGHIKLNDRPAKASAALKAGDAVRVVIPEVAPVEIVAQDIPLE
                     VLFEDKDILVLNKPPGLVVHPAAGNPDGTLVNALLHHCDDLSGIGGEARPGIVHRLDK
                     DTSGCMVVAKNDIAHRRLTEAFSERRLAKIYLAVINGIPLEKHGRIENRIGRHPVDRK
                     RMAVLYDGAGKEAVTEWEQRSEHQGCALIQCRLLTGRTHQIRVHMRECLGHPILGDPI
                     YGHPSRQRAPAARLMLHAWKLAFQHPIHDQPLSFEARVPEEFAPWMRRNES"
     gene            13668..14108
                     /locus_tag="PLP58_10535"
     CDS             13668..14108
                     /locus_tag="PLP58_10535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009961971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5069 domain-containing protein"
                     /protein_id="HRJ08999.1"
                     /translation="MSQIVPLISSGVAGPLGVLHLPRLWQKASLAAAGKLHPDYPGCG
                     KGYDGMTLAALGIAEADFLAFIQTKPTYTQFEAWVKAYPGVKLTKADIYKHNQSITGY
                     IHADDTRAAILAACGLPDDGSVNPGAVDLNNLDDWQTFWAAEIA"
     gene            complement(14281..16293)
                     /locus_tag="PLP58_10540"
     CDS             complement(14281..16293)
                     /locus_tag="PLP58_10540"
                     /inference="COORDINATES: protein motif:HMM:NF024907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VCBS repeat-containing protein"
                     /protein_id="HRJ09000.1"
                     /translation="MKTAARLGLLLAALSAASADDATLEPLQYNNPGLTVDLGVGLWA
                     WPMPMDFDGDGDLDLVVNCPDKPYNGIYFFENATGDTAKNPMPVFKPGVRISKGAQNV
                     RVSHVDGKPVVLSPNTIHPDFLKTGIEENVSLGLATNIHPNKVRANMWHLVDYDGDGA
                     HDLIVGVGDWTEYGWDNAYDENGRWMHGPLRGYVYLLRNKGTNDKPAYDKPKKVMAGD
                     RPVETYGWPCPNFADFDGDGDLDLLCGEFLDGFTYFENTGTRTQPKYALMKRVKTDTG
                     RFLTMDLEMITPTAIDWDKDGDLDLIVGDEDGRVAFIENTGTFDNKVPLFKEPRYFQQ
                     EAHLVKFGALATPVGFDWDGDGDMDIICGNTAGHVAFIENLSGPGVEKPKWAAPKHLE
                     AGGKIIRPMAGPNGSIQGPCEAKWGYTTQTVADWDGDGLPDLILNSILGKVVWYKNTG
                     TRTAPRLAAARPVEVEWEGPQPALAYGWMRPEGKALLTQWRTTPAAVDWNHDGLTDLV
                     MLDQEGYLAFFERSKKDGRLILKHPRRVLCADEKDPRPGLLNKALGALAVRNPVAMDP
                     GQPLRLNAGIAGKSGRRKLCIMDWDGDGKLDILLNSANANFLRQTGAKDGQWHFADMG
                     LLAAVNIEGHDVSPTTVDFNGDGIPDFLGGAEDGHLYYMRNPKAAK"
     gene            16624..17364
                     /locus_tag="PLP58_10545"
     CDS             16624..17364
                     /locus_tag="PLP58_10545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ09001.1"
                     /translation="MKKLILLQNDYPSTGKSTLAHCFSHYLKQFGVAHQRVTLLEDAE
                     NSDDEHALDASTITKRRLFDLMEEAPITLLESASGMGEFFCGFYQNHHLEQSLDEEGV
                     SLSVVLPVTTEVDSFDSVIEAVETFSDHAEYTIAHLVTSCYEEDERAWDRSYAARVMD
                     MYDAVELHIPEVGFQVELELRARHLSLSQALMDPGVTETFGKDYNKWLARVTGQIDSA
                     RRYLFGEAFRPTTQPRPVKKTRSRAKAA"
     gene            17533..18531
                     /locus_tag="PLP58_10550"
     CDS             17533..18531
                     /locus_tag="PLP58_10550"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HRJ09002.1"
                     /translation="MPRLSAAILLCACATLHALDLRLPTDNDALLTGDGPAFFQFVDR
                     DFEGRKSTPWEGGQFGFVRDARRVGSRVAFARFHEGMDIKPLRRDAAGNPLDEVRAIL
                     PGAVAHVSETSAHSNYGRYIVIRHDLSEGPFFSLYAHLGEIIVVPGERVQAGQRLARM
                     GFTGAGIDQRRAHLHLELNILLSEQFPAWHAEHFRTPNHHGLYNGLNLLGLDIQSLYL
                     ARRKNPALTLGEFLRGSEAYFELEVPGAARMEILRRYPWLNEGGSAEAPSWKVRCTRW
                     GFPVSVKPGSRSVATAFATWAREDPVPHYYNTRGLINNSGQITVEGLRFARLLCGF"
     gene            18536..19537
                     /gene="tsaD"
                     /locus_tag="PLP58_10555"
     CDS             18536..19537
                     /gene="tsaD"
                     /locus_tag="PLP58_10555"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009965177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HRJ09003.1"
                     /translation="MILALESSCDETAAAICAVDGRLLAARVSSQADIHRRYGGVVPE
                     VASRNHILHARPLVEQVLSDAGAALKDISAFAATSGPGLVSSLLIGASMAKAMAVAEG
                     KPFFAVNHMEGHLLSPFMEGAGPVRANVSLIVSGGHTMLVRVRAAGDYQLLGKTRDDA
                     AGEAFDKTAKMLGLPYPGGPEMDKQAALGNPRAFAFPRSFLDGRSLEFSFSGLKTAVL
                     YELPKLDLASPAVLADVCASVQEAIIEVLVEKLALAARQCGEKLVTVSGGVSCNRGLR
                     ARLASRFAEEGLTLLLARPDLCTDNAGMIAFAALQHFLSGRASPLDADVDPNLPLVA"
BASE COUNT         3650 a         6327 c         6327 g         3360 t
ORIGIN      
        1 gtttcgcatc gtgcgcccac cagacgaccg ggctaaagcc acagtcgtcc tacgcacatc
       61 attccggcga cgcacccatg ccgcgggagt ttgatctgga ggcgtttttt gaggtcttgg
      121 gaaacgccat ttcagttggc cgctaaaact tgaatttggt tggcaacgaa tggagccgga
      181 gtactctcgg ccgtccatgt cccagcttct cagccagcgt cttgcccgca gcatctcggc
      241 ggattttttc cggccgttgg cgcggccttc ggcgccggtg tatgtggatt gtgcggatcg
      301 tttgattgat gaggcgggcg aggcggggag gctttcgcag cgggagacga ttgagatcat
      361 caaggaagtc atcgcgcggc atccgctgtc gctgctggcg gaggatgagg gcgcggcgct
      421 gcgggatgcg cgtcagcgtg cggggcagtt tttcaatcgg ctcatcgccg cgggctggct
      481 ggaggatcag acgctcgggc tgcatgagcg ctgggcggtg atcacgccgg ggctgcgacc
      541 gctgctgcgc atgctgcgcg accttgcgga ggatcgcgtg gcggagctca agacgttcgc
      601 ggacacgctg cgcggtcttt gccagacgct ggaagagcgc ggcgtgctcg atccgcagat
      661 tcagccgccc gatcagctcc gctcaaccat caccgacctc acgcagcgtc tggagcgggc
      721 gatcgagcaa ctgcatgcgg tggaaaaaat cgtcaccggt tttgagcagc gccaaaggca
      781 gaccgccacg gccgcggaca cgctgaactt gctctacgag gagttcggcc aggggcagca
      841 catggtgtgt tacgatgcgc tgcgtcgcgg tggactgctc acccgcatcg aaaatgcacg
      901 cagcgctgtc tccgaatgcc acgatgatcc gctcatgcgc gagcgtctcg ccagcggcct
      961 gcgagagcat tacggctacg acgacgccga tgcctacgac cacgcggtgg cgatgctcac
     1021 gcggttggag cgcgatctcg gcggtctgaa acgccgcgcg gaggccatcg acctgcgcat
     1081 ggccgccttc aaccgcctca gccagcagcg ttaccgctac cagacggagc tgcgcggccg
     1141 tcggccggaa atcgtgaaag cttactgcga tgccatcaat gccgcgcatg cgggcggtcg
     1201 cttctccgag ttcgccggtc aaggcgccga cttcgcgccg cgctgtgtgg agacgcgttt
     1261 cttcttcggc accgagtcgc tctacaagcc acggcgcacg aaatcgccgg tcgatctgcg
     1321 ttttggcgaa accggtgcct cgaagcaaag cgaggacgat gtgctcgccg cgtggaaaga
     1381 acgccagcgc ctcgccctca cgccgcaacg tgccgcacga ctcgtggcga agcttttgcc
     1441 agccaaaggc cgcaccacca gcaccgagga catcaccctc gaaaccaccg acgacctgct
     1501 cgatctgctc gccgccgtgg cctacgagca cgcgcctgct ctcgccggca aacgcgtccg
     1561 ctggagcgtg gacggcctcc gccgccgcaa cggcctcgaa ccgcatattt tggagcacga
     1621 cgagatcgcc gggcaccgtg tggagcggtt tgcgatcacg cgggaggggt gatgcctttc
     1681 gacgcttggc aaatggttgc tacagactac gatcaagcca tgcagttcag ccaaagcccc
     1741 atcgaactcc atccactgat ggatctcatt cggcgtgacc gcgtcatcaa agcgcggaga
     1801 caaacgccgg aagagcgctt cgccgaagcg atggaaatga ccgacttcgc tttcgaggtg
     1861 atggcgtcat cggtgaagga ttcttatccc gatgctgacg cccagacggc gaccggcatc
     1921 ctgcgcaagc ggctgaaacg cctgcgcgat cgggaagacc gggggatttt tgttcctgcg
     1981 gggaaacaag tatgaaacaa agcgagctgg ttgccttcat tcttgaagca gcggaccgcg
     2041 ctggtgtctc ctgcatggtc ggaggatcgg tggcttgcat gaactacatg atccctcgtg
     2101 ccaccaagga cattgatctc gtcatccagg ttcaagagcc gcagttcacg gccttcctca
     2161 aggaggtgga ttcgcagttt gagttggatc cgcaggttta tgtggaaacg ctgacgggaa
     2221 cgaagcgcta catccttgag catcgcaaga cagcgttcga cgtggagctt tttattctca
     2281 gcagtgatcc tcatcacctc tccatgtgga gccggaaacg gctgcgtcgg caggttttct
     2341 gcacgcgcga tgcctggacg ccaacggccg aggacatggt cattcaaaaa ctccgctggg
     2401 cgcgtccgca ggacctcgtg gatgctgaca gcatgatcgt ggtgcagggc gatgaactcg
     2461 actggccgta tatcgaacac tggtgcgatc tgcatggcac gcgtgagcga ttggaaaaga
     2521 tccgggccga gattccaccg atggagtgag ctgaggctaa gatcggaggc agcatcatcg
     2581 gtgcgccttt catcttgcca cgcccccgcc ctgttctacc tctgccgccc ccatgacctc
     2641 cgccccctcc gatccgcttt ggccgcgctc tttttggagc gatgtgccgg attcggatcg
     2701 cgcggcgctg cgggaggtgc tggcggaatt gctggggcgt ggcgtgctgc tcggcgatga
     2761 gggcggcggg cgagagctgt atctgctggc ccgtgaccac taccgcacgc atgtggagga
     2821 ttacctcgcg ccgctggcgt tggagttgat cgtcgatgag gagcgctcgc tgctacaggc
     2881 gaggccgcgc acggagtcgt gcctgctgct gggccgtttt gacaaggacg agacgctcgt
     2941 gctgctggcg ctgtggcggc tttacgatga tgaaatcagc accggcagcc aggaggcggt
     3001 catcgtgacc gtggaccagc tctgggccgc gctgaaggtg tacttcgaca agatcagcac
     3061 gccggaaaaa tcgcagatcg aggcctgcct ggcccgcatc cgccgtcatc gcctcatccg
     3121 cacgctgagg cccgagggca tgacgcaggc gggcgagatg cagatcgaga ttttgccctc
     3181 gctggcacgc gtgatcccgt ttgatgacat cgccgcgtgg cagcagcgcg tggagcagta
     3241 tcagccggtc aaagacagcg cggaagacgg ggaggcggcc gcatgagcgc tccgcgcatc
     3301 accttgagcc gcatcctcgc ggtgaactgg tatggctacc gccagttcat cgacgtggcc
     3361 ggcctctcgc tcatcaccgg cgcgaatggc agcggcaaga gcgcactgct cgatctcatc
     3421 cagttcgtca tgctcggcga gcagcagagc aagttcaaca aagccgccgc cggcgctggc
     3481 agcggtcgct cgctgcgcgg ctactgcctg tgcgacacga acacgaccgg ccgcgatggc
     3541 gcggagcgct acctgcggcc cagcagcgtc acgctcgccg cgctggagtt cacctggccg
     3601 ctgcaatccg gtgaaaccga gctgcgtcgc gagacctggg gcgcacgcat cgagtatgaa
     3661 agccccacgg cgaagccttc gacgatctgg ttccgcgccg gacgccgcct cgcgtggcag
     3721 gattttctca acagcgacgc cggaccgcag gcgatgcaat ttctgcccga ggatgaattc
     3781 cgcacccgcg tgaagcgcga gctcgacggc gatgtgtggg accgccagca gacctatcgc
     3841 gatgagatgg cgctgcgcag ccacctcggc ttcgacgccg agcagatggc gaagacgctg
     3901 ccccgcgcga tggccttcga gccggagagc aacttcgaaa gattcgtgcg cgagttcctg
     3961 ctcgagcccg gcatgccgga tgtgaaggcg gtgaaggcca gcgtcgatgc gcatcgtcgg
     4021 gcacaggagc ggctggagaa aatgtacgac cagctcgagc gcctgaagcg catctccgcg
     4081 catcatcagg actggctcac ctcgaaacgc gagtccgcgc tctactcgca cctcagcgat
     4141 gctttgaagc acgaagaagc cctcgaaggc ctcaaccgcc gccgcgccga actggaagac
     4201 aaacgcgccg agcacgccga gaacctgcaa gcgcacgaac aggccatccg cgagcgcgaa
     4261 cgcctcgaac acgctgagga atccgcccgc gccgcgctgg gtgacaaggc cttcaagctc
     4321 gaagagatcg accgccgccg ccgcgagctc gacagcgaga tcgaacggct caacgcggcc
     4381 tccagcagcc ttcacgaacg cattcgcgcc catctccgcc gctggcagga ctggacgttg
     4441 catgcgtcgc gactcggcct gcaaaatctc gacgaggcct ccgccgccat ctccgcgatg
     4501 cagagcaagg acgagagcaa ggcgctcgcc gccgcacgcg ataccgcgcg tgcctacatg
     4561 cggctgaagg acgaggcgaa cgagcagctc cagcccgtgc tcgcccgcct cagcgagcat
     4621 gagctgcgaa aggccgcgct gcacaaagac ctcgcgcagc tccgcgaagg ccacgccacg
     4681 ccctcgccgc tgctcaatgc gctgctttcc cgcggacaaa aagccgtcgc gctcggtcgc
     4741 gtggtcgagg tgaaagccag cgcggaaaaa tggtggccgc tcatcgagac cgtgctcggc
     4801 attcatcgcc gcgccgtcat tcccgaggac ttccgcgccg cgtgggatca ggcgcagcag
     4861 acgcagaatc cgcacgagcc gctcattcat cccgaggaag ccgcgcaaac caccacgaag
     4921 gtgctcaaag gctcgctgcg tgagatggtg gagaccaaac acgatctcgc cggcaaggtg
     4981 ctcgatcacc tgctcggcgg cctcgtcgcc gtgaacaagg ccgcgcagct cgacaagcac
     5041 gagcgggcga tttcgctcga tggctggctc aaagatccgc cgcgtcgtgt gaagctcacg
     5101 cctgaaaaag agctcacgct cggcgaggaa ggcctgcgcc gccttcgcga cctgcgcgag
     5161 aacgagcttc gcgagaccga cgccgtcatc gaggaggtgc gcagcgattg cgatgatctc
     5221 cgcgcctttt taaaccgcgg cgctgaaggc catctcgacc gcttcaccgc gccggaaggt
     5281 gccgatgagg tccagcgcct gcccaaactg cgcgaggaac tccgcgagct gcaagagacc
     5341 tggaacctcc tcgccacgcc ggaccacgag aaggccatgg aaaacctccg cgtgcaaaaa
     5401 gccacgctca aaggcatcca cgagcgcatc ggcagcctca agaccgccat caccgctttc
     5461 acgctcgatc agtcgcgact cgaagaagcc atccaggagg atgtttttca ggaagagcag
     5521 acccgcatcg cccgccaggc ctcgcgcatg ctcgtgcatg gtttttccga cgaggagatc
     5581 accacgcgca tcaccgccga gcatcaaaag gctacgagct ggcgcaaatc catcgaaatg
     5641 gccgcagcgc tcgccattca gctcgacacc cgagcacaag agtcccgccg cttgcgcgat
     5701 gagcagcgtc gcgagctcat caccgtgcac gccgacctcg gcgatgcgct cgatgccgac
     5761 gcggaggaca acgcggcctt tgaccagcgc cgcgaggatc tcgaaacgca tgaattggag
     5821 cgcttcaaaa ccgcggcgga tgaagcgcgg aaggaatggg aggatcgttt gcagcatcag
     5881 gtgctcgacg tgctgcgtga aaagctcagc gaggccgacc gcacgaagcg cgagctgaac
     5941 cgcgccatgg atcacgagat tggcggctgg cgctaccagc tcaccagccg tgcggacaag
     6001 gcgcacagcg ccatctggac gctcgtcgaa aaaggcctgc ccagcggcag cgagatggag
     6061 ctcttcaacg ccgccggccg cgaggacatc gagcgggcca aggccgagct catggccgcc
     6121 atcgacgccg ccgacaaccc cgaggacaag cgccaccagc gcatgctcga ctaccgctac
     6181 taccaccact gggacatcga ggccaagccc gccggccgcg gcgatgccgc cgccatcagc
     6241 cttaacaaga gcgccaagaa acaaagcggc ggtgagaacc aggccccctt cttcgtcgcc
     6301 acgctcgccg ccttccgtcg cgtgtatgac ctcggccgtc gcgaggaaca gcagaatctc
     6361 ggcctcgtcg tcatggacga agccttcagc aaactcagcg gcgaccgcat cgacgactgc
     6421 ctcgcgctcg cccgcaactt cggcctccag ctcatcatgg ccttccccga agaccgcctc
     6481 cccacgatgt tccagcacgc cgacaccgtg gtccaatgcc gcgtcgaacg cagctacgac
     6541 gaatccaccg agcagatcca aaacatcgaa aactgggtgg tgcgagtaga aggctccagg
     6601 ctcgcggaat tgatggagtg atccttggac tgcggcagcc tgctgccgct ttcgccgagg
     6661 cagccctgct gccgccgaag tccgagagac gaactttcgg agatggacca cgtcttaaac
     6721 ccgccagcag ggctggccgc acccgaaagc ggcagcaggc tgccgcagtc catattgcct
     6781 tcgcacgctt gttcgccaac ggtgcctcat gcgctggctc caaaccctgc acgaacaatg
     6841 gatgaccgcc cgaaaacggc gggtggaggc atcggtgcgg gcgtttcggc gggattggga
     6901 aacgctgctc gatgacgcag gcctcacctc tgcggaagaa cgtcaggcag cccaacgaga
     6961 cgccgagaag ctcccgctga aactcatccc cttccgccac aagccgcgat tcatcgacaa
     7021 aatcgaagtc cccctcgaat ccgaagcctg gcttcatgcc cagtttggct cgcaaaccgg
     7081 agcggaggct caacaagccg ctttggccgt ggtggagcag ttttcagcca aaagccatcc
     7141 gctgcttccc gaggcttgga ccgctctttt aagccgtttg aaggccgaat ttgcccttcc
     7201 gcatgccgtg gagccgtttc gatggcgcga ggccactcgc gtcgaagagc tcttgaagct
     7261 gcttttcgac ctcacgagct gcgaatggcc cgaaggcacg ctgattcgcg atgcgagcac
     7321 gcagatcggc cgcgactcca aatacctcga agcgcagcag agcttcatcg agcgggcgct
     7381 ggagctgctg ctcggtcgcg agacgccgct ggaggcgctc ggcattcaga caagcaatag
     7441 cgtcttgcat tacagcggcc cgctcgtgct gcacttcgcc gatcacagca agccgctcga
     7501 cctccgcttc gagtccaccc tctccgtcgc cgagctggag cgtgccacgc acatcaccac
     7561 gaccgcgcag cgactcctca ccgtcgagaa tcgcaaaacc acctttctcc agctcacgcg
     7621 tgccgatgtc acacgcagca ccctcatcgt cgccacctcg ttcccgacgc aggccgtgcg
     7681 tctcctcctg caaaaactgc cacccgacct cccccaccat cacttcggcg acaccgaccc
     7741 cgccggctgg gacatcctcc gcaaactccg cgaggtctcc ggccgcaccg tccatccatt
     7801 ccacatgcac tggcgacccc aagccaacgc ctcacctctg acccggcgcg actatctgat
     7861 tctcacacgc ctcttttcca acccttcaat ggctgattgc cgcgccgcgt tcgaggacat
     7921 gaacgctgcg ggaacaaagg gcgattatga gcaggaatcc ctggggccgc cggagctgga
     7981 agcgtggccg ttttaccacg cgcttgaccg tggcgggcaa ccgctttaga gcagcaggcc
     8041 atgaacgcca aacctctcga aggaaagacc ggtctcgttt tcggagtcgc caacaagcgc
     8101 agcatcgcct gggccatcgc ccaggcatgg gcggaggcgg gcgccaagct cattttcaat
     8161 taccagggcg agcgcctgaa ggacaacgtc gaggaactgg ccggcacctt cggcacggac
     8221 acaccgctct ttccctgcga tgtctccaag gacgaggaag tggacgcctt tttcgacaaa
     8281 gtcgccgcca tcacgccgaa ggtggacctg ctcctgcaca gcgtggcctt tgccccgaag
     8341 gaagcgctgg agggcgattt cctcagcacc agtcgcgagg cctaccttac agcgcacaac
     8401 atcagcgcct actccctcgt ccacctcgcc cgcaaggccg cgccgctcat gacggacggc
     8461 ggcagcatca tcgccatgag ttactacggc gcggaaaaag tggtgccgca ctacaacgtg
     8521 atgggcgtgg ccaaagccag cctcgaggcc agcacccgct acctggccta tgacctgggc
     8581 aaaaagaaaa ttcgcgtgaa ctgcatcagc gccggcccgg tgaacaccct ggccgcgcgc
     8641 ggcatcgccg gcttcggctc catgctcaag cactacgatg agcacgcccc cctgggccgc
     8701 tcctgcgagc cgaaggagct gggcgccacc ggtgtcttcc tcgccagcga gggcgcggcc
     8761 tccatcaccg gccaggtgat ctatgtggac ggcggctacc agatcatggg catgtgaagg
     8821 cgtgaggctt ttgatgccag tcatcgccac aaatcctgag aacagcggtt ggagtcatgg
     8881 agtgctggag cattaaaaat ccatcactcc acaattccag gcatggattc acaaatcgtg
     8941 acaacccgcg gtttaaagcg ccttcgccgt cattccgttc ttcgctgaca cagcgtaaac
     9001 cagcaggccc gagaccatga tgccgagacc ggccagggat tcccatgtac gctctttgag
     9061 cacataaaca agcatccaca ggctgatgcc catgaagatc aacggagtca gcggatagcc
     9121 ccagcagcgc accggcctgc gcaggccggg ctgagtgcgg cgcagaacca tgacaccgag
     9181 caccgtcaga aaggagcata gctggatgct gaactgcacg caggtcagca ctttctcaaa
     9241 agtcgaggtc atcagcagcg cggcgacgat gagcgtctgc agggcgagcg ccacctgcgg
     9301 cgtgccgcgc ctgtccacac gcgccagcgg cgccagcagg cgcgcgtcct ggcccatcgt
     9361 catggccacg cgcggaccca cccaggtcat ggcgctcaca ctggagacca ggcccaggca
     9421 gatcagcccg ctcatcagtc tgccgccgga ttcgccgaaa agatggccgg ccgccacatg
     9481 ccccacctcc accctgccct ccagctcgtc catgggagcg gcatgaagaa agacggtgtt
     9541 cagcgccaga tacagcccag tgacaagcag ggtgccgagc gcgatggaga gcggcacatt
     9601 ccggcgcggc tcgcggatgt cgcccacgat gtaggtggtg gcgttccagc ccgcgtaggc
     9661 atacatcaca tacacgaggc tgacggcaaa aggcgtgctg gtcaccagcg cccagtcctc
     9721 cgccgccgga gtgaagcgcg cgccgtgccc ctctccgaga gcggcgcccg ccccgatgaa
     9781 gacgagaatg agcgccacct tcatccaggt ggccgcgttt tgaaaccgcg ccgccgtgtg
     9841 aatgcccgcc gaatgcacca ccgccaccac cagcaccatg aagactgaca acaccgtggc
     9901 ggacgctccg ggcacgacac cgccgaagta tttgccaaac gccatggccg ccagggcgat
     9961 gggcgcggcg aaacccaccg tgaacgaaat ccaccccgcc atgaaacccg ccgccggatg
    10021 gaatatcctg gccagaaaat gatactcccc tcccgatctc ggcatggcgg ccgccagctc
    10081 gccataagcc agcgctccgc aaaaagcgca cagcccgccc accgcccaca gcaccagcag
    10141 ggcgaagccg gacggcagtc ctccgacctg gaagccgagg ctggtgaaaa caccggtgcc
    10201 caccatgttc gccaccacga cggcgactgc ggtggagagg gaaatgccgg cgggcttcga
    10261 atcgggagtg ctcacggcgc ggagagtttc aaaacatgtg cgggcgtcaa ttgcgctttg
    10321 cccgcagctc ggtgcggatt ttgaagccgc tctggctgcg cagggggaag ggttcgggct
    10381 tgtcaggcgg gagggaggcg aggacggcgg agagctttgc gcgtgcggcc gcgacctcgg
    10441 ggcgggagtc ggatgacaag tcgtgctgct cgtcaggatc ggcgttggcg tcgaagaacg
    10501 gaccctcgga gtagagtttg aagcgggtgt cgcgcacgac gcgggtggtg tggtattcgt
    10561 tgaggatcca gtcgcgatgc ggaggcgctt tttcatcaac cagaaagcgg gcaaaggagc
    10621 tcccgtcggg caggcgggcg gcgtcaagcg gcacgccggc aagttcgcag aaggtggggt
    10681 agagatcggt gaagtcggcc agaggcagca ctcggccgcc ggggatgtgt ctggggttgt
    10741 tggcgatgag cgcgacgttc ccgccagcct cgttcatttt gtagaggccg cctttgacca
    10801 cgcgcccgga gcggcgggtg gagaggcttt gctcggtgcc gttgtcactg gcgacgatga
    10861 ggatggtgtc atcccgcagg ccgagctttt cgagttcctt taccatggcg tccacctgca
    10921 tgtccgcgta gcgcagcatg tcggcgaaca tttcctcatg cgggcggttt tcttcgcggt
    10981 tcagcggcgt ggtggtgaca gggtgggggt aggcgttgcc gtgggtgagc accatgggaa
    11041 ggtagagcat gaagggcttg tccttgtgcc ggcgcaggaa gtcgaaggcg aactcccgcg
    11101 ccacatccgt gccgaatttg ccgggatgcc gctcgcgctt tccctggcgg aggaagacgg
    11161 ggtcccagta gcggctttcg atgttttgaa tgaaggccac ggtgtcttcc acggactcgc
    11221 gcttcaccgc gtcccaaaag gcggcggtct gctccggggt catcttgtcc ttgtccaggc
    11281 tgccgggcca ggcaagatgc tcgtcgaagc cgtggcggtc gagcacgccg ggctcgtcgt
    11341 agaggttgtt cacctgccac ttgccggcga ggcaggtggc gaagccggct ttttggaaga
    11401 ggcggggaaa aaggatctcc cgctccggat cgagcccgcc gccgctgtaa agcgcggcat
    11461 cgtgatgcag gcggaagccg gtgctgtgcg ggtagcggcc ggtgagcgcg acgatgcggc
    11521 tggggccgca gacgggcgcg gtgtagcagt gctccacgcg cacgccggtg gcggccagtt
    11581 tatcaatcgc cggagtcacc ccggcttcgc ccccgtaaca gccgaaccat tcctggccca
    11641 cattatcgag cagcatgaag acgatgttgc gcgggcgggc ttcgatgaag ccgggcagca
    11701 gaacgagggc ggcgagcagg aaaatgtgtt tcatcaaagt cgcggtgtgc gggattctga
    11761 aaagcgtgtg tcaaggcgcg ccgcgctggt attcgcggat ggcggccaga gcgagcacct
    11821 cggtggcatt gaccacgggc agccgggtgc gggagctgtc gaaggcgagc gggatctccg
    11881 tgcaaccgag cacgatgacc tgcgcaccct tgcgggtgac atcgtccgcc gcgcgcgcga
    11941 gcaggtccgc ctggcggcgt ttgtcaccgc cggccttgat gctgtacacg gcgttcatca
    12001 cgcaggtttg ctggcaagcg gcgtcaggat acacgacacg aatgccgcgc gcttcgaagg
    12061 ccttctcata gagcttgctg cgcacggtgc cgttcgaggc cagcaggccc accaccttgc
    12121 aacgcgggtg ccgcttttcc acggcgtcca cggtctcggt gatcatgttc agcacgggga
    12181 cgcgcacggc cttctgcatg tccgcatggt aataatgaac cgtgttgcac gggatggcga
    12241 tgagcgaggc acccatgcgc tccagccggc gcacgccctc ctggatgtag ggcacgatct
    12301 cgcggctgcc ggactcgatg cacttcgtgc ggtcgggcac ctgcgggcgg ctgtaaatga
    12361 ctgtcggcag gtggtcctgg tcctccttcg ccggagtcag gcgcacgatc tcggcgtaga
    12421 gattggccgt ggcctccggc cccatgccgc caaagatgcc caccatgcgc ttcggcgcca
    12481 ctttgcacat gccgagttgc gagcacgaaa tgccagaagt gaaaagcagg gcggcgagaa
    12541 tgaggcgggc gggcatggga ttgcggaatc tgattttcga gcttccaacg aaacgcccct
    12601 tgccgggtga attgtcagtg gatgcgggaa aatccttttg ccacgccacc acccgcgccg
    12661 aacatgctcc cgtggagtgg agcatcgaac ccccggacag cggccagcgc ctcgacaaac
    12721 acctcgccgc caggctcggt gacctctccc gcgcgcgcat tcaggatctc atccgcgaag
    12781 gccacatcaa actcaatgac cggcccgcca aagccagcgc cgcgctcaaa gccggggacg
    12841 ccgtccgcgt cgtcatcccc gaggtcgccc ccgtcgagat cgtcgcgcag gacatccccc
    12901 tggaggtgct cttcgaggac aaagacatcc tcgtcctcaa caagcccccc ggcctcgtcg
    12961 tccaccccgc cgcaggcaac cccgacggca cgcttgtgaa cgccctcctg caccattgcg
    13021 atgaccttag cggcattggc ggcgaggccc gtcccggcat agttcaccgg ctcgacaagg
    13081 acaccagcgg ctgcatggtc gtggcgaaaa acgacatcgc ccatcgccgc cttaccgagg
    13141 ccttcagcga gcgccgcctc gccaaaatct acctcgccgt catcaacggc atcccgcttg
    13201 aaaagcacgg ccgcattgaa aaccgcattg gccgccaccc tgtggaccgg aagcgcatgg
    13261 cagtcctgta cgacggcgcg ggcaaggagg ccgtcaccga atgggagcag cgctccgagc
    13321 atcagggctg cgcgctcatc cagtgccgtc tgctcactgg acgcacacac cagattcgcg
    13381 tgcacatgag ggaatgcctc ggccatccca tccttggcga tcccatctac ggccacccct
    13441 cccgccagcg cgcgcccgcc gcccggctca tgctgcatgc atggaagctc gccttccagc
    13501 atcccatcca cgaccagccg ctgagtttcg aggcgcgcgt gccggaggag ttcgccccct
    13561 ggatgcgccg gaatgaatct tgaccaaacc cagcgctccg ccacgttcca tgcccggcag
    13621 cggaactccc tccgccgcca agtcctaacc tcatccccac acgaaccatg agccaaatcg
    13681 tccccctcat ctcctccggc gtcgccggtc ctctcggcgt cctgcatttg ccgcgcctat
    13741 ggcaaaaagc ctccctcgcc gccgccggca agctgcaccc cgactacccc ggctgcggca
    13801 aaggctacga cggaatgacc ctcgccgccc tcggcatcgc agaagccgat ttcctcgcct
    13861 tcatccagac caaacccacc tacacccagt tcgaagcctg ggtcaaagcc taccccggcg
    13921 tcaagctcac caaggccgac atttacaagc acaaccaatc catcacaggc tacatccacg
    13981 ccgacgacac tcgcgccgcc atcctcgccg cctgcggcct gcccgatgac ggctccgtga
    14041 accccggcgc cgtggacctc aacaacctcg acgactggca gactttctgg gccgccgaga
    14101 tcgcgtaaag cccgcaaaca ttccacttcc ccgcctccgc cggcccgccc ggcggaggcg
    14161 gctttttttg ggtattgtta acgcgccccg gcgcgttgct tgctcggtcc acatccacca
    14221 catgcggcaa gttctcgcaa acatcctcaa cgttccaacg cagcactggc ggagagtcgc
    14281 tcacttcgcg gccttcgggt tgcgcatgta gtagaggtgg ccgtcttcgg cgccgccgag
    14341 gaagtcgggg atgccgtcgc cgttgaagtc cacggtggtg gggctgacgt cgtggccttc
    14401 gatgttgacg gcggcgagca ggcccatgtc ggcgaagtgc cattggccgt ctttggcgcc
    14461 ggtctggcgg aggaagttgg cgttggcgga gttgagcagg atgtcgagct tgccgtcgcc
    14521 gtcccagtcc atgatgcaga gtttgcggcg gccgcttttg ccggcgatgc cggcattgag
    14581 cctgaggggc tggccggggt ccatggcgac ggggttgcgc acggcgaggg cgccgagggc
    14641 tttgttgagc aggccggggc gggggtcttt ttcatcggcg cagaggacgc ggcgggggtg
    14701 cttcaagatg agccggccgt cttttttgga gcgctcgaag aaggcgaggt agccttcctg
    14761 gtcgagcatg acgaggtcgg tgaggccgtc gtggttccag tccacggcgg cgggggtggt
    14821 gcgccactgg gtgaggaggg ctttgccttc ggggcgcatc cagccgtagg cgagggcggg
    14881 ctgggggcct tcccattcga cttcgacggg ccgggcggcg gcgaggcggg gcgcggtgcg
    14941 ggtgccggtg tttttgtacc agacgacttt gccgaggatg gagttgagga tgaggtcggg
    15001 caggccgtcg ccatcccagt cggcgacagt ctgtgtggtg tagccccatt tggcttcgca
    15061 gggtccctgg atggagccgt tggggccggc catggggcgg atgattttgc cgccggcttc
    15121 gagatgtttg ggggcggccc atttgggttt ttcgacgccg gggccgctga ggttttcgat
    15181 gaaggcgacg tggcctgcgg tgttgccgca gatgatgtcc atgtcgccgt cgccgtccca
    15241 atcgaagccg acgggggtgg cgagggcgcc gaatttgacg aggtgggctt cttgctggaa
    15301 gtagcggggc tctttgaaga gggggacttt gttgtcaaag gtgccggtgt tttcgatgaa
    15361 ggcgacgcgg ccgtcttcgt cgccgacgat gaggtcgagg tcgccgtcct tgtcccagtc
    15421 aatggcggtg ggggtgatca tttcgaggtc catggtgagg aacctgccgg tgtcggtctt
    15481 gacgcgcttc atgagggcgt atttgggctg ggtgcgggtg ccggtgtttt cgaaataggt
    15541 gaagccgtcg aggaactcgc cgcagaggag gtcgaggtcg ccgtcgccgt cgaagtcggc
    15601 gaagttgggg cagggccagc cgtaggtctc gacggggcgg tcgccggcca tgactttttt
    15661 tggcttgtcg taggcgggct tgtcgttggt gcctttgttg cggagcagat agacgtagcc
    15721 gcgcaggggg ccgtgcatcc agcggccgtt ttcgtcatag gcgttgtccc agccgtattc
    15781 ggtccagtcg ccgacgccga cgatgaggtc gtgcgcgccg tcgccgtcgt agtccacgag
    15841 gtgccacatg ttggcgcgga ctttgttggg gtggatgttg gtggcgaggc cgaggctgac
    15901 gttttcctcg atgccggttt tgaggaagtc gggatggatg gtgttggggc tgaggacgac
    15961 gggtttgccg tccacgtggc tgacgcggac gttttgcgcg cctttgctga tgcggacgcc
    16021 gggcttgaag acgggcatgg ggtttttggc ggtgtcgccg gtggcgttct cgaagaagta
    16081 gatgccgttg tagggcttgt cggggcagtt gacgacgagg tcgaggtcgc cgtcgccatc
    16141 gaagtccatg ggcatgggcc atgcccagag gccgacgccg aggtccacgg tgaggccggg
    16201 gttgttgtat tggaggggct ccagggtggc gtcatcggcg gaggcggcgc tgagggcggc
    16261 gaggaggagg ccaaggcgtg cggcggtttt catgggttgg gcggggatgg aaacgcgagc
    16321 atggcggcgc ttttgcgcgg ggcaaaccgg gggcgctgcc gatttttggc ggcgggttga
    16381 cgatgcttga ctttgccggg cgcggcggta ggcgatgcca ggaggagcgt cggcgcggca
    16441 gccgcagggg cgggacaaac aagtgcgcgc gcaggaagca aggcgtcccg tcggtgtgcg
    16501 ggaggcgaag caacggcaac aatagggcga agaggaggcg cgaaaattat ttgagagttg
    16561 tgaattatta ttgttgcaat aatttccata tgttttaaaa tcatcggtgc attcataaaa
    16621 aatatgaaaa agctcatcct cctccaaaac gactaccctt cgactggcaa gagcaccctc
    16681 gctcattgtt tcagccacta cctcaagcag ttcggcgtgg ctcatcaaag agtgacgctt
    16741 ctcgaagacg cggaaaactc agatgacgag cacgcgctcg atgccagcac gatcaccaag
    16801 cgacggttgt tcgatttgat ggaggaggcg ccgatcactc tgctggagtc agcgagtggc
    16861 atgggcgagt ttttctgcgg cttctaccaa aaccatcatt tggagcagtc gctggacgag
    16921 gagggagtga gcctctcggt cgtgctgccg gtgaccaccg aggtggacag ctttgactcg
    16981 gtgatcgagg cggtggagac cttctcggac cacgccgagt acaccatcgc ccatctggtc
    17041 acaagctgct acgaggagga tgaacgcgcc tgggaccgca gctacgccgc gcgtgtgatg
    17101 gacatgtacg acgcggtgga actgcacatc cccgaagtcg gcttccaggt cgagctcgag
    17161 ctgcgcgcgc gccacctctc actgagccag gcactgatgg accccggcgt gacagagacc
    17221 ttcggcaagg actacaacaa atggctggcc cgtgtgaccg gccagattga cagcgcccgc
    17281 cgctatctct tcggcgaggc cttccgcccg acgacgcagc cgcgcccagt gaaaaagaca
    17341 cgctcccgcg ccaaagcggc ctgacattcc cccccaacag ccagaataaa aacccgtcgg
    17401 agcgtgaagc acccagccgt ccccccggcg gtgcgacacg ctccgatcct tttgcagccg
    17461 gcagaatcgt ccactcctgg cgcctgccac gggatttgca ccgccgcgct tccgcatcta
    17521 tcaagccgca caatgccccg cctctccgcc gcaatcctgc tctgcgcctg cgccacactg
    17581 cacgcgctgg acctgcgcct gcccacggac aacgacgctc tcctcacggg agacggcccc
    17641 gcttttttcc agttcgtgga ccgggatttc gagggtcgaa agtccactcc atgggaaggg
    17701 ggacagttcg gttttgtccg tgacgcgcgc cgtgtcggca gccgtgtcgc cttcgcccgc
    17761 tttcatgagg gaatggacat caaaccgctg cgccgcgacg cggcggggaa tcccctcgat
    17821 gaagtccgcg ccatactgcc cggcgcggtg gcgcatgtca gcgagacgag cgcgcacagc
    17881 aattacggcc gctacatcgt catccggcac gacctttccg aaggaccgtt tttcagcctc
    17941 tacgcgcacc ttggcgaaat catcgtggtg ccgggagagc gcgtgcaggc cgggcagagg
    18001 ctcgcacgca tgggcttcac aggggctggg attgaccagc ggcgcgcgca tttgcatctg
    18061 gagctgaaca tcctgctctc ggagcagttc cccgcctggc atgcggagca cttccgcacg
    18121 ccaaaccacc acggtcttta caacggcctc aacctgctcg gactcgacat ccagtccttg
    18181 tatctggcgc gccggaaaaa ccccgcgcta acgctgggcg agtttctgcg cggcagtgag
    18241 gcctacttcg agctggaagt gccgggcgcg gcgcgcatgg agatcctgcg gcgttatccc
    18301 tggctgaacg agggcggctc cgcggaagcg ccctcctgga aggtgcgttg cacgcggtgg
    18361 ggcttcccgg tttccgtcaa acccggttcg cgctcagtgg ccacggcatt cgccacctgg
    18421 gcgagggagg accccgtgcc gcattactac aacacgcgcg gcctcatcaa caacagcggc
    18481 cagatcaccg tcgagggcct gcgctttgcc cgcctgctgt gcggatttta aacgcatgat
    18541 tctggcgctt gagtcctcct gtgacgagac cgccgccgct atctgcgcgg tggacgggcg
    18601 gctcctggcg gcgcgggttt cctcgcaggc ggacatccac aggcgctacg gcggcgtggt
    18661 gccggaagtg gcttcgcgca accacatcct gcacgcgcgc ccgctggtgg agcaggtgct
    18721 gtcggatgcc ggcgcggcat tgaaggacat ctccgccttt gccgccacca gcggccccgg
    18781 cctggtcagc tccctgctca tcggcgccag catggcgaag gcgatggccg tggccgaggg
    18841 gaagccattt ttcgccgtca accacatgga agggcacttg ctctccccct tcatggaggg
    18901 cgcgggaccg gtgcgggcga atgtctccct catcgtcagc ggcggccaca ccatgctggt
    18961 gcgcgttcgc gccgccgggg attaccagtt gctgggcaaa acccgcgacg acgccgccgg
    19021 agaggccttt gacaagaccg ccaagatgct cggcctgcct tatcccggcg gtcctgagat
    19081 ggacaaacag gccgcgcttg gcaacccgcg cgcattcgct tttccacgca gctttctcga
    19141 cggccgcagc ctggagttca gcttcagcgg gctgaaaact gccgtgcttt acgaactgcc
    19201 caagctggac ctggcgagtc ccgccgtgct ggccgatgtc tgcgccagcg tgcaggaggc
    19261 catcatcgag gtgctggtgg agaagctggc gctggccgcg cgccaatgcg gggaaaagct
    19321 tgtgactgtc agcggcggag tgagctgcaa ccgcggcctg cgcgcgcgcc ttgccagccg
    19381 cttcgcggag gagggcttga cgctcctgct ggcccggccg gacctgtgta cggacaacgc
    19441 gggcatgatc gccttcgccg cgctccagca ttttttgtca ggccgcgcct cgccgctgga
    19501 cgcggacgtg gacccaaacc tgccgctcgt ggcttgagcg gggccgcttt accacacgcc
    19561 gagcttttgc agttgccggg cggcctcgcc gggccagtcg cggttggcca gcgggctggc
    19621 gggactggga tgaaggatgc gccccacgcg gaagggacgc gcca
//
LOCUS       DAOMYJ010000084        19097 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Prosthecobacter sp. isolate AMR_MDS_5629
            NODE_1000_length_19097_cov_21.374803, whole genome shotgun
            sequence.
ACCESSION   DAOMYJ010000084 DAOMYJ010000000
VERSION     DAOMYJ010000084.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867850
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prosthecobacter sp. (wastewater metagenome)
  ORGANISM  Prosthecobacter sp.
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Verrucomicrobiaceae; Prosthecobacter.
REFERENCE   1  (bases 1 to 19097)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 19097)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.30
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:34:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,742
            CDSs (total)                      :: 4,684
            Genes (coding)                    :: 4,676
            CDSs (with protein)               :: 4,676
            Genes (RNA)                       :: 58
            tRNAs                             :: 55
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19097
                     /organism="Prosthecobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1000_length_19097_cov_21.374803"
                     /isolate="AMR_MDS_5629"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1965333"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..423)
                     /locus_tag="PLP58_10560"
     CDS             complement(<1..423)
                     /locus_tag="PLP58_10560"
                     /inference="COORDINATES: protein motif:HMM:NF018530.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ09004.1"
                     /translation="MSYLQSLQASAPVAHDILLFSIVILAGIGLGRVPFGGVRLGVAG
                     VLFSGLLASHCGLEPDGKVAHFLKDFGLVLFVFALGLQMGPSFFGSLKKDGLRLNGWA
                     AALVAGGAAVAVLGAWLLGLPLPAAAGLFAGATTNTPAL"
     gene            719..2164
                     /locus_tag="PLP58_10565"
     CDS             719..2164
                     /locus_tag="PLP58_10565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009958936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase"
                     /protein_id="HRJ09005.1"
                     /translation="MAFRSALIILCAITISLPLFSAPLNVLFIAVDDLRPEIGCYGAA
                     HMQTPHLDRLAAQGMLFERAYCQVAVCNPSRNSVLSGCRPDTTGIFDNQHFLRGEMPQ
                     VLTLPQHFKNHGYTTLSLGKVFHHSDREPGDDPQSWSEPAWYHGQPYRHWFTKASLDY
                     VKQLKALPKDKQPKQLRAAPFEAADEPDEAYPDGQTAVKAIETLQRLKTEAKPFFLAV
                     GFVKPHLPFTCPRKYWDLYPADSIHMPANAARPENAPGPAFHNNYELRSYGTVPENGK
                     IPDAMAPHLIRGYRACVSFMDAQLGKVLDELDRLGLRESTLIVLWGDHGYHLGENGLW
                     TKMTNFELGTRSPLMLCAPGMKTAGQRTKALVEFVDIYPTLAELCSLPLPKQLEGASF
                     APLLEKPDREWKKAAFSQYLRPGKPPVMGRSIRTRDWRYTEWHDPKQNLVGTELYDAA
                     ESKNLADDPAYSEVVKQLAGQLKAGWKAAQP"
     gene            2401..2790
                     /locus_tag="PLP58_10570"
     CDS             2401..2790
                     /locus_tag="PLP58_10570"
                     /inference="COORDINATES: protein motif:HMM:NF015898.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HRJ09006.1"
                     /translation="MTLSRVQLAILRVLWNCEEASVAEVQEALEDRALAYTTVSTLLS
                     RLELKGAVSHRAEGRTFYYRAKVREEDVSNTMVGELVKGVFGGSPAELVSHLLETHEV
                     DADELARIKEMVANHKATRRKGGSHGR"
     gene            2780..4987
                     /locus_tag="PLP58_10575"
     CDS             2780..4987
                     /locus_tag="PLP58_10575"
                     /inference="COORDINATES: protein motif:HMM:NF017390.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M56 family metallopeptidase"
                     /protein_id="HRJ09007.1"
                     /translation="MDASLHFGAWLLTYAIHSTLVIGAVWLWVRLARPRSPELRELLW
                     KAALVGGFVTTLAQGLLPFPHWGEVLLLARQDAVSATDVVPQSVAAPEGSAAIATLEA
                     QDRLEMAARHDDLISGAIGWLKQTFVDEAGLLWLWAALVLLGLLRMGCSWFGLLRLRR
                     GATRVHHGEIAECAHRIQRRLGSRRPLEIWITPAIRGPMAAGLTRWRLFLPQAFLQRL
                     DAEQREAVIAHELAHLARGDNWWLAAGQWIACLFFFQPLNRVVRRHLRTEAEYLADRR
                     ALQVLPDEMGLVRALVALGEWLVGVGRQPVFHPLATGMGACRSTLGKRVELLLEDGLG
                     HDASASMRRWVVALAVSALLLIAMAAPRAVASNPNPQSQPNSTMKSTLTSKAAALLAG
                     TALFAAVPAQAENEKPAATATIAALPETLHGFSGHLSGKLISKDVDGGSLVMQVNKLL
                     HVWKNNKSAKPEDIVGRSLTVTNIHSRSVDVLVTLQPGDHFEIEVKHVNGDTTMDIGE
                     GMKKVSAPDASESSASASPREAMHGFRGIFVGTLVEKDQEKGTLAVKIERIARVWKQN
                     TAKNAEAAAGQTWKINGVSGKWLDTLLTLKVGDRVEVEAFHHRGDELSFVGEWLKKAG
                     DEPAKEPAAKATGGRADLNGFRGLLVGKLLSKDIEKGEVEIEVDTVKTVFKKSTAPHP
                     ESAVGQKWRIQKIAGKFLDNLLIINVGERIELAAFHNHGEAMDFPGELLRKAE"
     gene            5033..7447
                     /locus_tag="PLP58_10580"
     CDS             5033..7447
                     /locus_tag="PLP58_10580"
                     /inference="COORDINATES: protein motif:HMM:NF019223.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1549 and DUF1553 domain-containing protein"
                     /protein_id="HRJ09008.1"
                     /translation="MTRPKRKGHRALALLCLFCSPVLLAETLAPVDQRIGSEGSLKID
                     FQRHVLPLLSRAGCNGRDCHGSAQGKGGFQLSLFGYDFDADHKALTKLVEKHGVARAD
                     KDNPAASLMLKKPAREVAHKGGKRIEKGGWQYELLLRWIQQGAPAQNRQQRFERLEVA
                     PAEIVFRQSGDTAVLSVVAVWADGTREDVTPLTRFQSNNDGVAAVEATGRVTARGAGD
                     THVVAFYDNGVAAVPVMQPLPGKSAHAVAATTVDRHIQTKLAKLGITPSKLCSDEEFL
                     RRASIDMTGTLPTPTEVEAFLANTRGDKRARKIDELLQRPAYAAWWANKLCDFTGNNP
                     QQQAELGQELASQWYDWIFRRIRENQPYDKLVADIVLATGRSEGQSYEEYAAEMSAYF
                     RASKPADFTGRPTMPHYWTRRTMTKPEDKALSFAHSFLGIRLQCAQCHKHPFDQWTQQ
                     DFKQFTRFFEPVKFDIPPAARETAKQLAAAGSATMNMQSMMMSQDMQAMAMDGRVVPW
                     RELYVEKGNRPELKLHLLGDRELTLRSDEDPREKIMTWMLEKDNPWFARAFVNRVWAN
                     YFNRGIVHPPDDFNPANPPSNEALLDHLAQGFVASGYDMKWLHREIVNSHAYQRSWEP
                     NATNAGDERHFSRAVPRRIPAEVVYDSLAQIVAAVGELEEVRENLDRRAVGHLSMRMA
                     GTYAMNIFGKPARATNSDCERSADPSLLQSLFLRNDPIMQGRLDDGGWLRELETRHSS
                     SAQPPRAELEPLIREAWLRVLSRPPRTTEQERALAHFSKAASTAGGMRDLLWALLNTK
                     EFILNH"
     gene            7453..8751
                     /locus_tag="PLP58_10585"
     CDS             7453..8751
                     /locus_tag="PLP58_10585"
                     /inference="COORDINATES: protein motif:HMM:NF019040.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1501 domain-containing protein"
                     /protein_id="HRJ09009.1"
                     /translation="MNTRSTSLIPRRRLLKVGALGAGLTLSQYLRLASAGATDASTKR
                     SGILVFLRGGPSHQDMFDLKPDASAEYRGEFNPIKTNVPGVEICEHLPKLARCADQYA
                     IVRGLTHNLADHGLGSTYLLTGNRPGSLVKYPTYGSVASAELSVMPDLPAFVSIEEPL
                     EGPGFLGIQHSALATGNKPRPNQPFSVRGVTLDGGLSLTQLENRQQLSRDLDTLFRGH
                     EKLSGEITAIDEFSDKAHRILSSARARKAFDLAAEPADIAGRFGAHETGQSLLLACRL
                     IEAGVRFVTVIVDNWDTHQQNFATLKRTLLPQFDQGLSALFTTLKDKGLLDTTAVLVT
                     GEFGRTPKINGTAGRDHWPRAMFSLMAGGAVHGGQVLGATDATASEPTDGGWSPDDIA
                     ASFYRNIGIDPHREYHIDTGRPIQLIRNGKPIEQIFTGAA"
     gene            8765..10345
                     /locus_tag="PLP58_10590"
     CDS             8765..10345
                     /locus_tag="PLP58_10590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ09010.1"
                     /translation="MNRRRFLQTAAATTTSPLIAADAPRKAPLRVLYSNDLTNITSCV
                     SPFHAAREPFRSEMLDASTDEVAGLVDAHFLQPGLGVVPLWPSKVIDLKEHFAWIKQR
                     YGQKPDSFSQFVLNGGDIVRRFIDHCRAKGQAAFISLRMNDAHHKEFVDPKPGDKPGS
                     SIGMSVTRWYAEHPDHRIKPGSLRGADLVLNWAEPEVRAQKLAMLRELCANYDLDGLE
                     LDFLRFYSYFRPETPLEQRRVIMTAFVKEVRRMLGPQKWLCARVPCYLTALDELGLDL
                     KALVAAGLDMVNASSHYFTTQQHDLAAIRKMTTGAALYFELCHTIWKGDKVQAGYDVF
                     PYRRATREHLHTSAHLAYARGADGISIFNFAYYRQHGQGDGRGVFGEPPFEALKALRE
                     PQTLAKQPQHWFIASGWRSPGAKPLPVPRKLAQGKTEKFNFDLASPRGDARVRIQMDR
                     TDATDLAAHFNGEPLTATNDVSEPFAVPFPSMLGKPEELRAWLIPARMLREGVNSLQL
                     VCHGPGAANILFLDLAVA"
     gene            10444..10518
                     /locus_tag="PLP58_10595"
     tRNA            10444..10518
                     /locus_tag="PLP58_10595"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:10476..10478,aa:Val,seq:gac)
     gene            10609..11418
                     /locus_tag="PLP58_10600"
     CDS             10609..11418
                     /locus_tag="PLP58_10600"
                     /inference="COORDINATES: protein motif:HMM:NF013793.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HRJ09011.1"
                     /translation="MIAIATASQKGGVGKTTLCINLAYSLARRGWNVLLADTDPQGGV
                     GLSLARSTRSKQGFYDYLCGGGDFDKLVLATRLPELQILPAGQYDACSRRGWPAHEVP
                     ARLADILRSAELRGVDCLIMDTAAGLNGMSESVVKACDYVIIPQQAEPLAVRSVPHML
                     ETLSRYRSEGAPVKVAGILLTMVMAGNSISQKVVNELRAMLPASLMFEQNVPRLESFL
                     EASAMGVPVALMKRNPPPEALIFDQIAAEIEQRTGLPEDREAAQSHASLLD"
     gene            11381..12202
                     /locus_tag="PLP58_10605"
     CDS             11381..12202
                     /locus_tag="PLP58_10605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ09012.1"
                     /translation="MKLLKAMQVSWIDPNEVSGLLAGLEPGRPAVSHDAPAPALPVIS
                     EAEAPGWSAWISELTDPPARAPVPETPDIFPSGHESAEDGEPALAETASEKEIDSIRE
                     RLRHIREQAVIAGLLQRRGTPVENATTVQERGNAARERDAFSTADLCFDAEGELGARL
                     ETFAEWARGRFKGADILVVDEHGDLLWGPPNAAGLVLSTLMALNSARRESAGAFSSIS
                     GLMTREIAPGRNLTLIACSSSLGLLQAAVVGSGVVSEEDAAVLREGLARVAGTED"
     gene            12366..14837
                     /locus_tag="PLP58_10610"
     CDS             12366..14837
                     /locus_tag="PLP58_10610"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HRJ09013.1"
                     /translation="MLPLRGAPDLEEDADFQSALAALRGGQALVAAAKSELLLERPSW
                     SAGERAGLAALAVEARVRAGDGAAALALLEREPVQGADYWRAQALALEGRHGEAEALL
                     TRRLERGDAGEREKLLLAQLFLRGANEVQARGMALQLLNSASEEVRPKALLILAEASL
                     AMGEAQAALEALPDSGETEAAHLRARALLDLGRGEEAEAVLRGIITARRGGESLVHSA
                     SLMRVEAGLESGRAAEALEALTAFLENTPRSTLWPHAFDLLARVLEELDTPPPDVIMR
                     WMVEGGAALRQSGAAEAEVALFRGHAMLALSRWLLHEGRKQEALGLLESMAVAHPEHP
                     QAVDAMRLALQTHGALGNDTAVAALASLWRGHFEEGGASFLDFITGGSAFVRGDYEDA
                     ADDFFAAANTAATLSARRAALFNACVAALRAGELLLYQTLLGQLQIVGSGSEVAVKSG
                     DSAADLELDRSLELASGGGQGAVSALNTFLENHASHPRALEACLALAELAMLSKPPNF
                     SMSEKALSQADVLTGLDDGQRQRVAVTRLWLLERQGKLEEAAALGGEFLKNWPGSDQE
                     DEVRMKVAEVYFRLEDFAAARTGFELLAQEHPESPYAESALYFAALSAMSMLTEEGRE
                     RALQLWQEIAERAGPLAQPALHQLAQARRLAGEEAEALRLTEMLLAGPDLAVDMRRQL
                     VCEKAEMLMLLGRVKPAHFDDAAAVLRAMLEGDGLPYAWRARAGHTLATVLHAAGQSA
                     EALAACHDVVEGAPFGGPANPAEFRWYYRAGFLGIELLEAARQWESAARLAEKLGSSR
                     GERAQEAAARATKIRLDHFIWDEKK"
     gene            complement(14849..15394)
                     /locus_tag="PLP58_10615"
     CDS             complement(14849..15394)
                     /locus_tag="PLP58_10615"
                     /inference="COORDINATES: protein motif:HMM:NF014417.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination NusG
                     family protein"
                     /protein_id="HRJ09014.1"
                     /translation="MIFSDQAAWHVIHGRPKCEHLAAAVMAELPEVETYCPRIRYQKS
                     TRRGRVWFVEALFPGYFFARFAPAKSARAVRHSQNVIRIVEFGGMMSPVPDSVIMGIR
                     AEMEGREVREIHLEVRPGDEVEVAEGPMRGIKGIVNHVHDGARRVRILMEILGGENVV
                     EVDASKLLPARQPREALAGGA"
     gene            complement(15435..17063)
                     /locus_tag="PLP58_10620"
     CDS             complement(15435..17063)
                     /locus_tag="PLP58_10620"
                     /inference="COORDINATES: protein motif:HMM:NF015585.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialate O-acetylesterase"
                     /protein_id="HRJ09015.1"
                     /translation="MIHRLLPVFLLALTTLAARAEIKLPALFSNGMVFQQGRPVPVWG
                     SAGPDEDVTVVFAGQTQVTRADLDGRWRVTLDPLLVSAQPAELTVSGNGTVVVKDILV
                     GEVWLCSGQSNMQWTVAQSANPEQETAAAKYPQIRMFSVERATAMTPARDVAGAWKTA
                     TPENAAQFSAVAYYFGRHLHNALKVPVGLVNTSWGGTRVEAWTSREALEDRPCAAQLL
                     SDWDGILQSWDGAAETARFEVARAAWKEQVDKLKAENEKLPAGQKKPLPSAPRAPDDP
                     GKTPHFPAALFNTMVSPLIPYAIQGAIWYQGESNQNRAVQYQELLPTLINDWRTRWNS
                     EFSFYIVQLAGYGNRKPATSDPGVDDTWAELQEAQYLTAITLPGCGLAVTNDIGEKDD
                     IHPKNKQEVGRRLALWALAKDYGRADTVHSGPLFKNSMIEGGKVRLQFDHVGGGLKTR
                     DGGPLKHFQIAGADQKWHWAEAVVSGDEVHVSSPQVPAPAGVRYAWAAWPEGANLVNA
                     EGLPASSFRTDEFIPSTLGVTSPFQELQKAVPAR"
     gene            complement(17116..18369)
                     /locus_tag="PLP58_10625"
     CDS             complement(17116..18369)
                     /locus_tag="PLP58_10625"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009959931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent rRNA methyltransferase"
                     /protein_id="HRJ09016.1"
                     /translation="MPRPPARQPARSFQRPPAAPKPPPPGSEDWERPWAQIKYYTFHP
                     AIYPNMVATVSADAGAGDLVRVYDKEGKPFGSGWLNPKARVPLRVLHHGADSFGEDDL
                     DARVRQAARLRTQVLKPGAETDAFRAVHSDADGLPGLVVDRYADTLSIEVTTLGAWRR
                     LRRWLPLLHEELGTTRHVIHTDSDIALIESMRRADVPEADEPGPRSVKIREHGVRYLV
                     NFEEGHKTGFFCDQRDNRARLGRLARGRVLDLCTYTGGFALAARLPGGCEDVTGVDLD
                     EKAIAQARQNANLNQTRVQWVHADAFGWARQMIKNGELWDTVVLDPPKLIHHRDKQEE
                     GIFKYRDLNGLALQLVRRGGLFVTCSCSGLLGTDDFEQLVIGSAHRQGKKLQILDRTG
                     PGMDHPVMSNCPESRYLKVIWAVVV"
     gene            complement(18503..18688)
                     /gene="rpmF"
                     /locus_tag="PLP58_10630"
     CDS             complement(18503..18688)
                     /gene="rpmF"
                     /locus_tag="PLP58_10630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018970685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HRJ09017.1"
                     /translation="MANPKRRQSKSRQRLRQGANRWRAPVLKTCPECGTTVPGHVACP
                     SCGYYRGRQVLTITAEE"
     gene            complement(18702..>19097)
                     /locus_tag="PLP58_10635"
     CDS             complement(18702..>19097)
                     /locus_tag="PLP58_10635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ09018.1"
                     /translation="DLAPGDSVQAASPLAYDLRILREGDSLHVSGSLEAGFSLQCGRC
                     LERFPHRVRLENFEEEAPLENESTTDLTELVREDILLALPSYPRCEDGNVEQRECPAE
                     GKFERDDTPDADGLPGAERGIWNALDQLK"
BASE COUNT         3410 a         6031 c         6268 g         3388 t
ORIGIN      
        1 cagtgcgggg gtgttggtgg tggcgccggc aaagagcccc gctgccgccg gcagaggaag
       61 acccagcagc cacgctccca gcacggccac cgccgccccg cctgccacca gcgccgctgc
      121 ccagccgttc agccgaagcc cgtccttttt cagcgagccg aaaaacgacg gccccatctg
      181 cagccccaga gcaaagacaa acagcaccag cccgaaatcc ttcaaaaaat gcgccacttt
      241 gccatccggc tccagtccgc aatggctggc cagcagcccg ctgaacagca cgccggccac
      301 ccccaggcgg acgccgccga acggcacgcg cccaaggccg atccccgcca ggatgacgat
      361 gctgaaaagc aggatgtcat gcgccaccgg ggcgctggct tgcagggact ggaggtaaga
      421 cacgttgata gcagggcata aataatgcca gcgagacgct aatcttttgc cacaaacggc
      481 caacatcgga cggaaagtga gtttgaggcg ggatctcggc agcctttggt caaaacttca
      541 ttccatgcca ccgccgtcgc tcccatatcg gatgccatga ttgtctggcg cggcatcatc
      601 aagccaggga gggagagcat tgcggacact ttggcttaga acctcatgct catggagatc
      661 ggggcggttt gccagcctgt ttcacgaaat ggccatcctg atttctcgtt tgaggcacat
      721 ggcattccgt tccgctctca tcattctttg cgcgatcaca atatcgcttc cgctcttttc
      781 agcacctctg aatgtcctct tcatcgcggt ggatgatctg cggcccgaaa tcggctgcta
      841 cggcgcggct cacatgcaga cgcctcatct cgaccgactc gccgcgcagg ggatgctgtt
      901 tgagcgggcg tattgccagg tggcggtgtg caatccctcg cgcaacagcg tgctcagcgg
      961 ctgccggccg gacacgacgg gcatttttga caaccagcac tttctgcgcg gcgagatgcc
     1021 gcaggtgctg acactgccgc agcatttcaa gaaccacggc tacaccacgc tctccctcgg
     1081 aaaggtgttc catcacagcg accgggagcc gggcgacgat ccgcagtcat ggagcgagcc
     1141 cgcctggtat catggccagc cttacaggca ctggttcacg aaggcatcgc tcgattatgt
     1201 gaagcagctc aaagcgctgc cgaaggacaa gcagccgaaa caactgcgcg ccgcgccctt
     1261 tgaggccgct gacgagccgg acgaagccta tcccgacggg cagaccgccg tgaaagccat
     1321 cgaaaccctt cagcgcctca aaaccgaagc gaagccgttc ttcctcgccg tgggttttgt
     1381 gaagccgcac cttcccttca cctgcccgcg gaagtattgg gacctgtatc cggcggactc
     1441 gattcacatg cctgcaaatg ccgcgcggcc cgaaaacgcg cccgggcctg cgtttcacaa
     1501 caactacgaa ctgcgcagct acggcaccgt gccggaaaac ggcaaaattc ccgatgcgat
     1561 ggccccccat ctcatccgcg gctatcgcgc ctgcgtgagc ttcatggacg cgcagctcgg
     1621 caaggtgctg gatgagctgg accgcctcgg tttgcgcgaa agcaccctca tcgtgctctg
     1681 gggcgaccac ggctaccacc tcggcgaaaa cggcctctgg acgaagatga ccaacttcga
     1741 actgggcacg cgctctccgc tcatgctctg cgcccccgga atgaaaaccg ccggtcagcg
     1801 caccaaggcg ctcgtcgagt ttgtggacat ctatcccact ctcgccgagt tgtgctcgct
     1861 gccgctgcca aagcagctcg aaggcgccag ctttgcccca ttgctcgaaa agccggaccg
     1921 cgaatggaaa aaagccgcct tcagccaata ccttcgcccc ggcaagccgc cggtcatggg
     1981 ccgcagcatc cgcacccgcg actggcgcta caccgagtgg catgatccga agcagaacct
     2041 tgtcggcacc gagctttacg atgctgccga gtccaaaaat ctcgctgatg acccggcgta
     2101 ttcagaagtt gtcaaacagc tcgccgggca gttgaaagca ggctggaaag ctgcacagcc
     2161 ttgatctttc acatcatcgc ggctgataca tgctgggact gctgcaggcc actctcttga
     2221 aggtgtagtg tagggaggag gactttggtg gccggcttcg tgcgagaagg ttgccgcttt
     2281 ggtgtcgtgg tgggaagaat agcatcattt actcaccgct aaaaagacgt tgacttactt
     2341 ctacagttgt agttcaagtg gcggccaccg tccgagtcca ccatccaatc cattgcgacc
     2401 atgacactta gccgagttca acttgcgatt ctccgggttc tctggaactg cgaagaggcc
     2461 agcgtggctg aggttcagga ggcgttggaa gatcgggcgc tggcctacac cacggtatcc
     2521 acgctgctgt cacggctgga gctgaagggt gcggtgtcac accgggcgga gggacgcact
     2581 ttctactacc gcgcgaaggt gcgcgaagag gatgtcagca acacgatggt gggtgagctg
     2641 gtgaaagggg tgttcggagg aagcccggcg gagctggtga gccatctgct cgaaacgcat
     2701 gaggtggacg cagacgaact ggcgcggatc aaggagatgg tggcgaacca caaagcgaca
     2761 cgacggaagg gaggcagcca tggacgctag cctgcacttt ggagcgtggt tgctgaccta
     2821 cgcgattcat tcgacgctgg tgatcggcgc cgtgtggctg tgggtgcggc tggcgcgtcc
     2881 ccggtcgccg gagttgcgcg agcttttgtg gaaggcggcg ctggtcggcg gattcgtcac
     2941 cacgcttgcg cagggtttgc tgccctttcc gcactggggc gaggtgcttt tgctggcgcg
     3001 gcaagatgca gtgtctgcaa cggatgtcgt gccgcaatca gtggcggcac ccgagggaag
     3061 cgcggcgatt gcaaccttgg aggcgcagga ccgcttggaa atggcggccc gtcacgatga
     3121 cctgatttct ggagcgatcg gctggctcaa gcaaaccttc gtggacgagg ctggcctgct
     3181 ttggctgtgg gcggcgctgg tgttgctggg tctgctgcgc atgggctgta gctggttcgg
     3241 attgctgcgg ctgcgtcgcg gagccacgcg agtccaccac ggcgagatcg cggaatgcgc
     3301 gcaccgcatt cagagacgcc tcggcagtcg cagacccttg gaaatctgga tcacaccggc
     3361 cattcgcggc ccgatggcgg cgggtctgac gcgctggcga ttgtttctgc cgcaggcctt
     3421 tctccagcgt ctggatgccg agcagcgcga ggcggtgatc gctcatgaac tggctcatct
     3481 ggcgcgtggt gacaactggt ggctggcggc cggccagtgg atcgcttgtt tgttcttctt
     3541 ccagcccctg aaccgcgtgg tccggcgtca tctgcgcacg gaggcggaat atctggccga
     3601 caggcgtgcg ctgcaggtgc tgcctgatga gatgggcttg gtgcgcgcgt tggttgcgct
     3661 gggtgagtgg ctcgtcggcg tcggccgcca gccggtgttt catccgctgg ccaccggcat
     3721 gggcgcctgc cgctccacac tcggcaagcg ggtggagctg ctgcttgaag atggcctggg
     3781 gcatgatgca tccgccagca tgcgtcgctg ggtcgtcgcg ctggccgttt ccgccctgct
     3841 tttgattgcc atggccgcgc ctcgcgccgt cgcctcgaat ccgaatcccc aatcccaacc
     3901 caactccacc atgaaatcga cactcacatc caaagccgcc gccctgctgg cgggcaccgc
     3961 gttgtttgcc gccgttccag cgcaggctga aaacgaaaaa ccggccgcca cggcaaccat
     4021 tgccgcgctt cctgaaactc tgcacggctt cagcggtcat ctctccggca aattgatcag
     4081 caaggacgtg gatggcggtt cgctggtgat gcaggtgaac aagctgctgc atgtgtggaa
     4141 aaacaacaag tctgccaagc ccgaggatat cgtgggccgc agcctcacgg tgacgaacat
     4201 ccactcccgc tccgtggatg tgctggtgac gcttcagccg ggcgatcatt tcgaaatcga
     4261 ggtcaagcat gtgaacggcg acaccacgat ggatatcggc gaaggaatga aaaaggtatc
     4321 cgcgccggac gcgagtgaat cctcggccag cgcatcgccc cgcgaggcga tgcacggctt
     4381 ccgcggcatt tttgtcggca cgttggtcga gaaggatcag gagaagggca cgctggcggt
     4441 gaagatcgag cgaatcgccc gagtgtggaa gcagaacacc gccaagaacg ccgaggcggc
     4501 cgcaggccag acttggaaga tcaacggcgt gtccggcaag tggctcgaca ccctgctgac
     4561 gctgaaagtc ggcgaccgcg tcgaggtgga ggccttccac catcgcggtg acgagctcag
     4621 cttcgtcggc gagtggttga agaaggcggg ggacgaaccg gcgaaagaac cagccgccaa
     4681 ggcgacggga ggccgggcgg atctgaacgg cttccgcggc ctcctcgtcg gcaagctgct
     4741 ctccaaggac atcgaaaagg gcgaggtgga gatcgaggtg gacacggtga agacggtctt
     4801 caaaaagagc acggccccgc atccggagag cgcggtgggc cagaagtggc gcatccaaaa
     4861 gatcgccggc aagttcctcg acaacctgct gatcatcaat gtcggcgagc gcatcgaact
     4921 ggcggctttc cacaaccacg gagaggcgat ggatttcccc ggcgagctgc tgcgcaaggc
     4981 cgagtaacgc gacggcaacc ccgctgatct catcaccatg agggcgaaga ccatgactcg
     5041 accaaaacgc aaaggacacc gcgccctggc tttgctctgc ctgttttgca gccctgtgtt
     5101 gcttgcagaa actctcgcgc cggtggacca gcgcatcggc agcgagggct ccctgaaaat
     5161 cgactttcag cgccatgtgt tgccattgtt gagccgagcg ggatgcaacg ggcgtgactg
     5221 ccacggctcc gcgcaaggca agggcggctt ccagctctca ctgttcggct acgacttcga
     5281 cgcggaccac aaggcgctca ccaagctcgt ggaaaaacac ggcgttgcgc gtgcggacaa
     5341 ggacaacccc gctgccagcc tgatgctgaa aaaaccggca cgggaggtcg cgcacaaagg
     5401 tggcaagcgc atcgagaagg gaggctggca atatgagctg ctgctgcgct ggatccagca
     5461 gggcgcgccc gcgcagaacc ggcagcagcg tttcgaacgc ctcgaggtgg ctcctgcgga
     5521 gatcgttttc cgccaaagtg gtgacaccgc cgtgctgagc gtggtggccg tgtgggctga
     5581 cggcacgcgc gaggatgtga caccgctgac gcgctttcag agcaacaacg acggcgtcgc
     5641 cgcagtcgag gccacaggcc gtgtcacggc acgcggcgcg ggcgacacgc atgtcgtcgc
     5701 gttttatgac aacggtgtcg ccgcggttcc ggtgatgcag ccactgcctg gaaagtccgc
     5761 ccatgccgtg gcggccacga ccgtggaccg ccatatccag acaaaactcg ccaagctcgg
     5821 catcacgcct tccaagttgt gcagcgacga ggagtttttg cgccgcgcga gcatcgacat
     5881 gaccggcacg ctgcccacgc cgacggaagt ggaggcgttt ctcgccaaca cacgcggtga
     5941 caagcgcgcg cgcaaaatcg acgagctgct gcagcgcccc gcctacgccg catggtgggc
     6001 gaacaagctg tgcgacttca ccggcaacaa tccacagcag caggccgagc tcggccagga
     6061 gctcgcctcg cagtggtatg actggatctt ccgccgcatc cgcgaaaacc agccgtatga
     6121 caagctggtc gccgacatcg tgctcgccac aggacggagc gaggggcaga gctacgaaga
     6181 atacgccgcc gagatgtccg cctacttccg cgcgtcgaag cccgccgatt tcaccggacg
     6241 cccgaccatg ccgcactact ggacgcggcg cacgatgacg aagccggagg acaaggcgtt
     6301 gtcctttgcg cacagtttcc tcggcatccg cctgcagtgc gcgcagtgtc acaagcatcc
     6361 cttcgaccag tggacgcagc aggatttcaa gcagttcacc aggttcttcg agccggtgaa
     6421 attcgacatt ccgcccgccg cacgagagac ggcgaaacag ctcgccgccg ccggcagcgc
     6481 gacgatgaac atgcagagca tgatgatgtc gcaggacatg caggccatgg cgatggatgg
     6541 gcgcgtggtg ccgtggcgcg agctttatgt ggagaaaggc aaccgtcccg agctgaagct
     6601 gcaccttctc ggagatcgcg agctgacgct tcgttccgat gaagatccgc gtgaaaaaat
     6661 catgacgtgg atgcttgaga aggacaaccc gtggttcgcc cgcgccttcg tcaaccgcgt
     6721 gtgggcgaac tatttcaacc gtggcatcgt ccatccgccg gatgacttca accccgccaa
     6781 tccaccctcc aatgaggcgc tgctcgacca tctcgcgcag ggatttgtcg ccagcggcta
     6841 cgacatgaag tggctgcacc gtgaaatcgt gaacagccac gcctaccagc gcagttggga
     6901 gccgaatgcg accaacgctg gcgatgagcg ccatttcagc cgcgcggtgc cgcgccgcat
     6961 ccccgccgag gtggtgtatg actcgctggc ccagatcgtg gccgcggtcg gtgagctcga
     7021 agaggtgcgc gaaaacctcg accgccgcgc cgtggggcat ctttccatgc gcatggccgg
     7081 cacctacgcc atgaacatct ttggcaagcc cgcgcgcgcc acgaactcgg attgcgagcg
     7141 ctcggcggac ccctcgctgc tgcagagcct ctttctgcgc aacgatccca tcatgcaagg
     7201 ccgtctcgac gacggcggct ggctgcgtga gctggaaacg cggcactcct ccagcgcgca
     7261 accgccacgt gcggaactgg agccgctcat ccgcgaggcc tggctgcgcg tgctgtcgcg
     7321 accgccgcgg accacggagc aagagcgagc tctcgcgcat ttttccaaag ccgccagcac
     7381 cgccggtggc atgcgcgatc tgctttgggc gctgctcaac acgaaagaat tcatcctcaa
     7441 ccattgaatc gcatgaacac gcgcagcacg tccttaatcc cccgccgccg tcttctcaaa
     7501 gtcggagcgc tcggcgctgg gctcacgctc agccagtatc tgcggctcgc cagcgcgggc
     7561 gcgacggatg cctcgaccaa acgctcgggc atcctcgtgt ttctgcgcgg cgggccgtcg
     7621 caccaggaca tgttcgatct caagcccgac gcttccgctg aataccgcgg cgagttcaac
     7681 cccatcaaga ccaacgtgcc cggcgtggag atctgcgagc acctgccaaa gctggcacgc
     7741 tgcgcggatc aatacgccat cgtgcgcggc ctcacgcaca acctcgccga ccacggcctc
     7801 ggcagcacct acctgctcac cggcaatcgt cccggctcgc ttgtcaaata tcctacctat
     7861 ggctctgtcg cgagcgcgga gctgtccgtc atgccggatc tgccggcgtt cgtcagcatc
     7921 gaagagcctc tcgaaggccc cggtttcctc ggcatccagc acagcgcgct ggccactggc
     7981 aacaagccgc gcccgaacca gcccttcagc gtgcgtggcg tcacgctcga cggcgggctg
     8041 agcctgacgc agctcgaaaa ccgccagcag ctctcgcgtg atctcgacac gctgttccgc
     8101 ggccacgaaa aactcagcgg cgaaatcacc gccatcgacg aattttccga caaggcgcat
     8161 cgcatcctca gctccgcacg cgcacgcaaa gctttcgatc tcgccgccga gccagcggac
     8221 atcgccggcc gcttcggcgc gcacgagacc ggccagagcc tgctgctcgc ttgccggctc
     8281 atcgaggccg gtgtgcgctt cgtcacggtg atcgtggaca actgggacac gcatcagcag
     8341 aacttcgcca cgctcaagcg cacactgctg ccgcagttcg accaggggct ctccgcgctg
     8401 ttcaccacgc tgaaggacaa gggcctgctc gacaccaccg ccgtgctcgt caccggcgaa
     8461 ttcggccgca cgccaaagat caacggcacc gcaggccgcg accactggcc ccgcgccatg
     8521 ttctcgctca tggccggcgg cgccgtgcat ggcgggcagg tgctcggcgc caccgacgcc
     8581 accgcctccg agccgaccga cggcggctgg tcccccgacg acatcgccgc gtcgttttat
     8641 cgcaacatcg gcatcgatcc gcatagggaa taccacatcg acaccggccg cccgattcaa
     8701 ctgatccgca acggcaagcc gatcgagcag atcttcaccg gcgcggcttg atgcgaataa
     8761 gctcatgaac cgccgacgct ttctccaaac tgccgctgcg acaacaacat cgccgttgat
     8821 cgctgcggat gctcctcgta aagcgccgtt gcgcgtgctg tacagcaacg acctcacgaa
     8881 catcacctct tgcgtaagtc cgtttcatgc ggcgcgggaa ccgtttcggt ccgagatgct
     8941 cgatgcgtcg actgatgaag tcgcggggct ggtggacgcg cactttttgc agccgggact
     9001 cggcgtggtg ccgctgtggc cgagcaaggt gatcgatttg aaggagcact ttgcatggat
     9061 caaacagcgc tacggccaga agccggactc gttcagccag ttcgtgctca atggtggcga
     9121 catcgtgcgg cgcttcatcg accactgccg cgcgaaaggc caggcagcct tcatctcgct
     9181 gcgcatgaac gacgcgcatc acaaagagtt cgtcgatccg aagcccggcg acaaaccggg
     9241 cagcagcatc ggcatgagcg tgacgcgctg gtatgccgag catcccgatc atcgcatcaa
     9301 accgggctct ctgcgcggtg cggacctcgt tttgaactgg gcggagcccg aggtgcgtgc
     9361 gcagaagctc gccatgctcc gcgagctgtg tgcgaactac gacctcgacg gcctcgaact
     9421 cgatttcctg cgcttctaca gctacttccg ccccgaaaca ccgcttgagc aacgacgcgt
     9481 catcatgacc gccttcgtca aagaagtgcg ccggatgctc gggccgcaaa aatggctctg
     9541 cgcgcgtgtg ccatgctatt tgaccgctct cgatgagctg ggcctcgatt tgaaagcgct
     9601 cgtcgccgcc ggtctcgaca tggtgaacgc ctcctctcac tacttcacca cgcagcagca
     9661 tgatctggcc gccattcgga agatgaccac gggtgccgcg ctctacttcg agctctgcca
     9721 caccatctgg aaaggcgaca aggtgcaggc gggctacgac gtgtttccgt atcgccgcgc
     9781 cacgcgcgag catctccaca cgagcgcgca tctcgcctat gcacgcggcg cggacggcat
     9841 cagcatcttc aacttcgcct actaccgaca gcatgggcag ggcgatggcc gcggcgtgtt
     9901 cggcgaaccg ccgtttgagg cgctcaaagc cctgcgcgag ccgcaaacgc tcgccaaaca
     9961 gccgcagcac tggttcattg cgagcgggtg gcgttcaccg ggggcgaagc cgctgcccgt
    10021 gccgcgcaag ctggcgcagg gcaagacaga gaagttcaac ttcgacctcg caagccctcg
    10081 tggtgatgcg cgggtgcgga ttcagatgga tcggaccgat gcgaccgatc tggcggctca
    10141 tttcaacggg gagccgctga cagccacgaa tgatgtctca gagccttttg cggtgccgtt
    10201 tccatccatg ctgggcaagc ctgaggaact gcgcgcgtgg ctgattccgg ccaggatgct
    10261 gcgcgagggt gtgaactcgc tgcagcttgt atgccacgga ccgggcgcag cgaacatcct
    10321 tttcctcgat cttgccgtgg catgactgga tttgcgcggt ggtttttgtc gtgtttggag
    10381 attgaaaaaa tcggttgccc gcacctgcat aggcggtaac taatcggccc tcgatggaac
    10441 atcgcgcgat tagctcagtg gtagagcatc accttgacat ggtgggggtc gcaggttcga
    10501 accctgcatc gcgcaccatc cccagcggcg gaggggattt tcacaggagt gagccgtgca
    10561 agccaagaca gccttgccac cacggcatcg cgctttagca tccggccagt gatcgccatt
    10621 gccacagcaa gtcaaaaggg cggcgtgggc aagaccacgc tgtgcatcaa tctcgcctac
    10681 tccctagcca ggcgcgggtg gaacgttctc ctcgcggaca ccgacccgca gggtggcgtg
    10741 ggcctatcct tggcgcgctc cacccgctcc aagcagggct tctacgacta cctttgcggc
    10801 ggcggggatt tcgacaagct cgtgctggcc acccgcctgc ccgaactgca aatcctgccc
    10861 gccggccagt atgacgcctg ctcccgccgg ggctggcccg cgcatgaggt gccggcccgt
    10921 ctggctgaca tcctgcgctc cgccgaactg cgcggcgtgg actgcctcat catggacacc
    10981 gccgcgggcc tcaacggcat gtccgagagc gtcgtcaagg catgcgacta cgtcatcatt
    11041 ccccagcagg ccgagcctct ggcggtgcgc agtgtgccgc acatgctgga gacgctctcc
    11101 cgctaccgca gcgagggggc gccggtgaag gtggcgggca tccttttgac gatggtcatg
    11161 gccggcaaca gcatcagcca gaaagtcgtc aacgaactgc gggccatgct gcccgccagc
    11221 ctcatgttcg agcagaatgt gccccgtttg gaatcctttc tcgaagccag cgccatgggg
    11281 gtgccggtgg cgctgatgaa acgcaacccg ccgcccgagg cgctcatctt cgaccagatc
    11341 gcggcggaga tcgagcagcg cacaggcttg ccggaggatc gtgaagctgc tcaaagccat
    11401 gcaagtctcc tggattgatc cgaatgaggt ctccgggttg ctcgccggac tggagcctgg
    11461 gcggcctgcc gtgtcgcatg atgctcccgc gcccgcgctt ccggtaattt cggaggcgga
    11521 agctccgggg tggagcgcct ggatttccga acttacggac cctcccgctc gtgctccggt
    11581 gccagaaaca ccggacatct ttccttccgg acatgagtcg gccgaggacg gggagccggc
    11641 actcgccgag actgcctccg aaaaggagat tgactccatc cgcgagcgac tgcgccacat
    11701 tcgcgagcag gccgtcatcg ccggactcct gcaacggcgc gggactccgg ttgaaaacgc
    11761 cacgactgtt caggagcggg gaaatgccgc gagggagcgg gacgcattca gcaccgccga
    11821 tctttgtttt gacgcggagg gggagctcgg agccaggctg gagacattcg cggaatgggc
    11881 gcgcggccgc ttcaaaggcg cggacatcct tgtggtggac gagcatggcg acctgctctg
    11941 gggaccgcca aacgccgccg gactggtgct ctcgacactc atggcattga actcggcgcg
    12001 gcgtgaaagc gcgggggcgt tcagcagcat atcgggtctc atgacccgcg aaatagcccc
    12061 cggccgcaat ctcacgctca tcgcctgctc cagcagccta ggccttctcc aggcggctgt
    12121 tgtcggcagc ggtgttgtct ccgaggaaga tgccgctgtt ctacgggaag ggctggcccg
    12181 ggttgccggg acggaggatt gagatggcgg cagactcccg ccttgatcca ggacagcatc
    12241 gggcttttta gcctggcatg gctgacggtg gagaaagacg tttggaaaac gggccggcca
    12301 tgctgtagcc tgcgcgtctc atgcgagatg ccttgcgacc tttgatcctc tgccttttgc
    12361 ccgccgtgtt accactgcgc ggggcgccgg atttggagga agacgcggat tttcagtccg
    12421 cccttgccgc gctgcgcggc ggacaggctt tggtggctgc ggcgaagagc gagttgctgc
    12481 tggaaaggcc gtcctggtcc gcaggcgagc gggccggtct ggcggcgctg gcggtggagg
    12541 cgcgggtgcg cgcgggcgac ggggccgccg ctcttgcctt gctggagcgt gaaccggtgc
    12601 agggcgcgga ttattggagg gcgcaggcgc tggccttgga gggcaggcat ggcgaggcgg
    12661 aggctttgct gacgcggcgt ctggagaggg gcgatgcggg ggagcgggaa aaactgctgc
    12721 ttgcccagtt gttcctgcgc ggcgccaatg aagtccaggc gcgcgggatg gccctgcagt
    12781 tgctgaactc cgcctccgaa gaggtgaggc ccaaggcgct gctgatcctg gcggaggctt
    12841 cgctggcgat gggcgaggcg caggcggctc tggaggcgct gccggactcc ggagagaccg
    12901 aagcggccca tctgcgggcg cgcgccctgc tggatctggg acgcggggag gaggcggagg
    12961 cggttttgcg cggaatcatc accgcgcgcc ggggcgggga gtccctggtc cactccgcca
    13021 gtctgatgcg ggttgaggcg gggctggagt caggccgcgc tgcggaggct ttggaggcac
    13081 tgacggcctt cctggaaaac actccgcgca gcaccttgtg gccgcatgcc tttgatctgc
    13141 tggcgcgggt gctggaggag ctggacacgc cgccgccgga cgtcatcatg cgctggatgg
    13201 tggagggcgg cgccgcgctg cgccagtccg gcgcggctga ggctgaagtc gccctgtttc
    13261 gcggccatgc gatgctggcg ctgtcccgct ggctgcttca tgagggccgg aaacaggagg
    13321 cgctgggtct gttggagagc atggctgtgg cgcatcctga gcatccccag gcggtggacg
    13381 cgatgcgcct ggcccttcag acccacgggg cgctcggcaa cgacacggcg gtggccgcgc
    13441 ttgcctcctt gtggcgtgga cacttcgagg agggcggcgc gtcattcctc gacttcatca
    13501 caggcggcag cgccttcgtg cgcggggatt atgaggatgc ggcggatgat ttcttcgccg
    13561 ccgcgaacac ggccgccacg ctctccgcgc ggcgagcggc gcttttcaat gcctgtgtgg
    13621 cagcgctgcg ggcgggcgag cttttgctct accagactct gctgggacag ttgcaaatcg
    13681 tcggttcggg gtcggaggtc gcagtgaaaa gcggggacag cgccgccgac ctggagctgg
    13741 accgatccct ggagctcgcc tccggcggcg gccagggcgc ggtgtcggcg ctcaacactt
    13801 ttctcgaaaa tcacgcctcc catccccgcg ccctggaggc atgcctggcg ctggccgagc
    13861 tggccatgct gtcaaagccg ccaaatttct cgatgtctga aaaagcgctg agccaggcgg
    13921 atgtgctcac gggacttgat gacgggcagc gccagcgtgt ggcggtgaca cgcctctggc
    13981 ttctggagcg ccagggaaag ctggaggagg cggcggctct gggcggggag tttttgaaaa
    14041 actggcccgg ttccgaccag gaggacgagg tgcgcatgaa ggtggccgag gtgtatttcc
    14101 ggctggagga ttttgccgcg gcgcgcacgg gtttcgaact gctggcgcag gagcaccctg
    14161 agtcgcctta tgcggagagc gccctgtatt tcgccgccct ctccgccatg tccatgctca
    14221 ccgaggaggg gcgcgagagg gcactgcaac tctggcagga gatcgcggag cgcgcgggtc
    14281 cgctcgccca gccggcgctt caccagctcg ctcaggcgcg tcgtctggcg ggcgaggagg
    14341 ccgaggcttt gaggctgacg gaaatgctgc tggccgggcc ggatctggcg gtggacatgc
    14401 gccgccagtt ggtttgtgag aaggcggaga tgctcatgct gctcggcagg gtgaagcccg
    14461 cgcatttcga tgacgccgcc gccgttctgc gcgcgatgct ggagggggat ggcctgccct
    14521 acgcctggcg cgcccgcgcc ggtcacaccc tagccaccgt gctccatgcc gcaggccagt
    14581 ccgccgaggc gctggcagct tgtcatgacg tggtggaagg cgcgcccttc ggcggtcccg
    14641 ccaatcccgc cgagttccgc tggtattacc gcgcgggttt cctgggtatt gagctgcttg
    14701 aggcggccag gcagtgggaa tccgccgcgc gcctggctga aaaactcggc tccagccggg
    14761 gggagcgcgc gcaggaggcc gccgcgcgcg ccaccaagat ccggctcgac catttcatct
    14821 gggatgagaa aaagtgatac gcgttgtttt acgcgccgcc ggccagcgcc tcacgcggct
    14881 ggcgggccgg gaggagcttg gaagcgtcca cctcgaccac attttccccg ccgaggattt
    14941 ccatgaggat gcgcacacgg cgcgctccgt catggacgtg gttgacgatg cctttgatgc
    15001 cgcgcatcgg cccctcggcc acctccacct cgtctccagg gcgcacttcg agatggattt
    15061 ccctgacctc ccgcccctcc atctccgcgc ggatgcccat gatcaccgag tccggcaccg
    15121 gtgacatcat gccgccgaac tccacgatgc ggatgacatt ttgcgagtgt cgcacagcgc
    15181 gcgcggattt ggcgggggcg aaacgggcga agaagtagcc ggggaaaagc gcctcgacaa
    15241 accacaccct gccgcgccgg gtgctttttt ggtagcggat gcgcgggcag taggtctcca
    15301 cttcaggcaa ttccgccatc acggcggcgg ccagatgctc gcacttcggc ctgccatgaa
    15361 tgacatgcca ggccgcttgg tcggagaaaa tcacccgtca tcatggagcg ccacccgcga
    15421 ctgggcaagg cggcttatct ggctggcacg gctttttgca gctcctggaa aggggaggtg
    15481 acgccaagag tgcttgggat gaattcatcc gtgcggaagc tgctggcggg caggccctcg
    15541 gcgttgacga ggttggcgcc ctccggccag gctgcccagg cgtagcgcac gccggcgggc
    15601 gccggcacct gcgggctgct gacgtggact tcgtctccgg agacaaccgc ctcggcccag
    15661 tgccattttt ggtcggcacc ggcgatctgg aagtgcttca aaggcccgcc gtcgcgggtt
    15721 ttgagaccgc cgccgacatg gtcgaactgc aggcgcacct tgccgccctc aatcatggag
    15781 tttttgaaaa gcggtccgct gtgcacggtg tccgcgcggc catagtcctt cgccagcgcc
    15841 cagagggcga ggcggcggcc cacctcctgt ttgtttttcg gatggatgtc gtccttttcg
    15901 ccgatgtcgt tggtgacggc gaggccgcag ccggggaggg tgatggctgt caaatactgc
    15961 gcctcctgga gttccgccca ggtgtcgtcc actccggggt ctgaggttgc gggtttgcgg
    16021 tttccgtagc cggcgagctg gacgatgtaa aagctgaact cgctgttcca gcgcgtgcgc
    16081 cagtcgttga tgagcgtggg cagcagctcc tggtattgca cggcgcggtt ttgattgctc
    16141 tcaccctgat accagatggc gccttggatg gcatacggga tcaagggcga gaccatggtg
    16201 ttgaagagcg cggcggggaa gtgcggtgtt ttgccaggat catccggcgc gcgcggcgcg
    16261 gagggcaggg gctttttctg gccggcggga agtttttcgt tttcagcctt gagcttgtcc
    16321 acctgctcct tccaggcagc gcgcgccact tcgaacctgg ccgtctccgc ggctccgtcc
    16381 cagctttgca ggatgccgtc ccagtcgctc aggagctgcg cggcgcaggg ccggtcctcc
    16441 agggcctcgc ggctggtcca tgcctcgacg cgggtgccgc cccaggaggt gttgacgagg
    16501 ccgacgggga ctttgagggc gttgtgcaga tgcctgccga agtagtaggc cacggcggaa
    16561 aactgcgcgg cgttttccgg cgtggcggtc ttccatgcgc cggccacgtc ccgggcgggt
    16621 gtcatggcgg tggcgcgctc gacactgaac atgcggatct gcggatactt ggcggcggcg
    16681 gtttcctgct ccgggttggc ggactgcgcc acggtccact gcatgttcga ctggccggag
    16741 cagagccaca cctcgccgac gaggatgtct ttgacgacca cagttccgtt gccggagacg
    16801 gtcagctcgg cgggctgcgc gctgacgagc agcggatcga gtgtcacgcg ccatctgccg
    16861 tcgaggtcgg cgcgtgtgac ctgggtctgg ccggcgaaaa ccacggtcac atcctcatcg
    16921 ggaccggcgc tgccccagac ggggacgggc cgcccttgct ggaacaccat gccattgctg
    16981 aaaagcgctg gcagcttgat ctcggccctg gctgccaggg tggtcagggc gaggagaaag
    17041 acggggagca gtctgtggat catggcgggg aaaaggggaa agaaacgcgg cggctggccg
    17101 gctttgcaga aaaaatcaaa ccacgacggc ccatatcact ttcagataac ggctctccgg
    17161 gcagttcgac atgaccgggt ggtccatgcc ggggccagtg cggtcgagga tttgcagctt
    17221 cttcccctgc cggtgcgcgc tgccgatgac aagctgctcg aagtcgtccg tgccaagcag
    17281 ccccgagcat gagcaggtga cgaacaagcc gccgcggcgc acgagctgga gcgccaggcc
    17341 gttgagatcg cggtatttga aaatgccctc ctcctgtttg tcccggtggt ggatgagctt
    17401 cggcgggtcg agcaccacgg tgtcccaaag ctcgccgttt ttgatcatct ggcgcgccca
    17461 gccaaaggcg tccgcatgca cccactgcac acgcgtctgg ttgaggttgg cgttttgccg
    17521 cgcttgggcg atggcctttt catccaggtc cactccggtc acgtcctcgc agcctcctgg
    17581 aaggcgcgcc gccagggcga agccgcccgt gtaggtgcag aggtcgagca cacgcccgcg
    17641 cgccagcctg ccgaggcggg cgcggttgtc ccgctggtcg cagaaaaagc cggtcttgtg
    17701 cccttcctcg aagttcacca gatagcgaac accatgctcg cggattttca ccgagcgcgg
    17761 ccccggctcg tccgcctcgg gcacatccgc gcggcgcatg gactcgatga gcgcaatgtc
    17821 cgaatccgtg tggatgacgt gccgcgtggt gcccagctcc tcgtgcagca ggggcagcca
    17881 tcggcgcaga cgccgccatg cgccgagggt tgtcacctcg atggagagcg tgtccgcgta
    17941 acggtccacg accaggccgg gcagaccgtc cgcgtccgaa tgcaccgcgc ggaaggcgtc
    18001 tgtctcagcg cccggcttca gcacctgcgt gcgcagccgc gcagcctggc ggacgcgcgc
    18061 gtcgaggtca tcctcgccaa aggaatccgc gccatgatgc agcacgcgca gcggcacgcg
    18121 cgcctttgga ttgagccagc ccgagccgaa aggcttgccc tccttgtcgt aaacccgcac
    18181 caaatcaccg gcgccagcgt ccgccgaaac ggtggcaacc atgttcgggt aaatggccgg
    18241 gtgaaaagtg taatatttga tctgcgccca gggccgctcc caatcctcgc tgccaggggg
    18301 cggcggtttt ggagccgcgg gcggacgctg aaatgaacgg gcgggttgtc tggcgggagg
    18361 gcggggcatg gagggaatgc gagtttatac cggcttgtgt gtaatgcggg gactttttgc
    18421 cgtgactccg gcagcttgcc ggcaaaagaa caaccccgtc cggcgtgccg ggcggggttg
    18481 cgaaagtcgg aaacgggcgg gattactcct cggcggtgat ggtcagcacc tgacggccgc
    18541 ggtagtagcc gcaggagggg caggcgacat ggccggggac ggtggtgccg cattcagggc
    18601 aggtcttcag gacgggggcg cgccagcggt tggcgccctg gcggaggcgt tggcggctct
    18661 tggattgtct gcgtttggga ttggccatgt ctgtggggtg gttatttcaa ttgatcgagg
    18721 gcgttccaaa ttccgcgctc cgctccgggc agtccgtctg catcaggtgt gtcgtcacgt
    18781 tcgaatttgc cctccgcagg gcattcgcgc tgctcgacgt tgccgtcttc gcagcgcgga
    18841 tagctcggaa gggccaggag gatgtcttcc ctgaccagtt ctgtcaagtc cgtcgtggac
    18901 tcgttttcca gcggtgcctc ctcctcgaaa ttctccaggc gcacgcggtg ggggaagcgc
    18961 tccaggcagc gtccgcattg caggctgaac ccggcctcca ggctgcctga gacatgcagg
    19021 ctgtctcctt cccgcaggat gcgcaggtcg taggccagcg gcgaggcggc ctgcacactg
    19081 tcaccgggag cgaggtc
//
LOCUS       DAOMYJ010000085        18844 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Prosthecobacter sp. isolate AMR_MDS_5629
            NODE_1014_length_18844_cov_21.506626, whole genome shotgun
            sequence.
ACCESSION   DAOMYJ010000085 DAOMYJ010000000
VERSION     DAOMYJ010000085.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867850
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prosthecobacter sp. (wastewater metagenome)
  ORGANISM  Prosthecobacter sp.
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Verrucomicrobiaceae; Prosthecobacter.
REFERENCE   1  (bases 1 to 18844)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 18844)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.30
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:34:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,742
            CDSs (total)                      :: 4,684
            Genes (coding)                    :: 4,676
            CDSs (with protein)               :: 4,676
            Genes (RNA)                       :: 58
            tRNAs                             :: 55
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18844
                     /organism="Prosthecobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1014_length_18844_cov_21.506626"
                     /isolate="AMR_MDS_5629"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1965333"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..711
                     /locus_tag="PLP58_10640"
     CDS             <1..711
                     /locus_tag="PLP58_10640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ09019.1"
                     /translation="AQPGAVFRQLGAGAVLYLASDDLWRWRFQVADLYHQRLWMQLAA
                     WIAAPPFQAENDRVSIGADRLRHLPGETAEIRVRLRDADGALISSAQPRAILIHEGRE
                     IATLTLEPDPTHYGIYRATTPPLKAGAYEVAVSEGPAAARAEPRLSLRVADTGSTEWA
                     SLTMNRPLLEAVASASGGRFLREEQAATDLPNLLQAIDRRQTTVRETNLWSSWWWFGA
                     VICLLTAEWLLRKKLRLV"
     gene            806..1156
                     /locus_tag="PLP58_10645"
     CDS             806..1156
                     /locus_tag="PLP58_10645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ09020.1"
                     /translation="MIEGIQATVSAEELRELCLKHARQHEASVQQLTRELEGGKGRKK
                     KTRTRREMEDDTLNLIGYHSNKALEMKFFADHLEPGEKYMLDRNDLCQLGIIQEDREF
                     NRGWSDPFEDEIPF"
     gene            complement(1190..1642)
                     /locus_tag="PLP58_10650"
     CDS             complement(1190..1642)
                     /locus_tag="PLP58_10650"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018970631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008175 - tRNA methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (cytidine(34)-2'-O)-methyltransferase"
                     /protein_id="HRJ09021.1"
                     /translation="MLHVVLYQPEIPHNTGAVGRLCLATGARLHLIKPLGFSLEDKYL
                     KRSGLDYWQQVDVRVWEGWENFEREHGGARMAFIECQGERPHWEADFSGAGELCLVYG
                     PETRGIPPSILERHPGASWHIPMRPGARSLNLSTAVAITLYEAARQRR"
     gene            complement(1738..2694)
                     /locus_tag="PLP58_10655"
     CDS             complement(1738..2694)
                     /locus_tag="PLP58_10655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009962019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxy-5-phosphonooxypentane-2,4-dione
                     thiolase"
                     /protein_id="HRJ09022.1"
                     /translation="MADLSTSAADKKEKEFFTHVPQEAPGFFLKGSHQYDWGLKNRLS
                     RVFNPRDGRTVMLAFDHGYFQGPTTGLERVDQTILPLEPYADCLMLTRGIQRSIIPAS
                     TQKAIALRASGGTSMVSPFEEWEGEIGDKKFKLGRPGFEPLSNESTALSIEEAIRLNA
                     SVLAVQVFIGSAYERQSLKNLTDLVDAASRYGIGVMGVVAVGRAMARNAQYFRLATRI
                     MAELGANVVKCYYTDEGFDTITSCCPVPIVIAGGKKLPELDALTMCHNAIQQGASGVD
                     MGRNIFQSDAPVAMMQAVRGVVHEGLTPAQGYQMYQDLKTGG"
     gene            3132..3814
                     /locus_tag="PLP58_10660"
                     /pseudo
     CDS             3132..3814
                     /locus_tag="PLP58_10660"
                     /inference="COORDINATES: protein motif:HMM:NF013866.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
     gene            complement(3822..4286)
                     /gene="tnpA"
                     /locus_tag="PLP58_10665"
     CDS             complement(3822..4286)
                     /gene="tnpA"
                     /locus_tag="PLP58_10665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009958672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
                     /protein_id="HRJ09023.1"
                     /translation="MPQSLANILLHAVWSTKDRFAFLTDKPLRDELHRYLGGISARLD
                     CPTLIVGGVADHVHILLRLARTITVADWVKEMKRASTVWLIDQAHRDPMLSKFHWQSG
                     YGIFSVSESKTDDVRAYIANQEEHHAKHTFQDEYRRFLQAHRIEWDEKHVWD"
     gene            complement(4738..6027)
                     /locus_tag="PLP58_10670"
     CDS             complement(4738..6027)
                     /locus_tag="PLP58_10670"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HRJ09024.1"
                     /translation="MAHTINLLQTTNPDGIHSLLTQFPSPKSIKGKITIKLGNAYLYS
                     CVFPIIAAWRKSLPDGVEVEMNDNSLEEAAKRLLQNVGIVDIVEQNTEAPALVYSKTG
                     NVPLQPIVRGHSVESTVNKIYKMVDEWAGYLRDISAFRVILTELCENVLVHSGSTTPG
                     YLHAKVHRSQHGDKCEITIADSGIGIRNSYLEGTNEEVKQRLLKANASAVEIALDGLN
                     SSKPREITPGGSSHFGYGLFTIKRLIELNRGHLTVVSGDEFVTVNRFKTEKKKLQTPW
                     SGTIISLLLDLANPLPLEDVYEEEVSKIIPEPQTPPQVGTVALVETASVQPAPSKTSF
                     PLLQFGTELLTREIGLAIRAELATTLLDANVIEVDLDGIVDIMPSVADEVFGKLSLRL
                     GVEKYRSRIIFRGGTPVLHRLIDFVVAQRAKGENASS"
     gene            complement(6099..6206)
                     /locus_tag="PLP58_10675"
     CDS             complement(6099..6206)
                     /locus_tag="PLP58_10675"
                     /inference="COORDINATES: protein motif:HMM:NF012584.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ09025.1"
                     /translation="MFAAVASSSAGTVIEVCVKIGESVESKDLLVRLVK"
     gene            complement(6229..6426)
                     /locus_tag="PLP58_10680"
     CDS             complement(6229..6426)
                     /locus_tag="PLP58_10680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ09026.1"
                     /translation="MPTKNSQTETAAFDAVAASREWKQTVAETTAGMTMAERMAWFRS
                     QSSIPAIRSRAKVPRRLKAHT"
     gene            complement(6401..6880)
                     /locus_tag="PLP58_10685"
     CDS             complement(6401..6880)
                     /locus_tag="PLP58_10685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ09027.1"
                     /translation="MPDPARKLYIDTSVVGGYHDPLWMADTRLLWQQAKAGHWSLITS
                     IVAEAEIQNAPANVRQIFADTFDASNILDTSHEVEELAEAYVQAAVVPPKFADDALHV
                     AMATVHGVRLIVSWNFKHLVNVRREDGFNAVNILRGWPIVRIVSPKEIIYADQEQPD"
     gene            complement(6908..10447)
                     /locus_tag="PLP58_10690"
     CDS             complement(6908..10447)
                     /locus_tag="PLP58_10690"
                     /EC_number="6.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009960218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004736 - pyruvate carboxylase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009374 - biotin binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006090 - pyruvate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate carboxylase"
                     /protein_id="HRJ09028.1"
                     /translation="MPKKSAAKSAVSASAPPAIRPIKKLMVANRSEIAIRVMRAATEL
                     GIRTVGIYAQEDRFCPHRFKADEAYELNKDKGPLGAYLDVEGIVTLAKEKGVDAIHPG
                     YGFLSENPDFSKACEKAGIIFIGPDPKILTMMGDKTAARNVARKLNVPILEGTDEPVS
                     DRKEAIAVAKKIGFPLIIKAAFGGGGRGMRIVREAKDLEKLLDEAQTEAERAFGNGAV
                     FLEKFVGKAKHIEVQILADKHGTVLHLHERDCSVQRRHQKVIEQAPSYGVKQEIIDGL
                     CDAAVKLAEEVKYTHAGTVEFLVDHETGEWYFIEMNPRIQVEHTVTEEITSIDIVRSQ
                     ILIAQGYQMHQEPLGLPPQEKIEKSGFAIQCRITTEDPENAFTPDFGRILNYRSPGGF
                     GVRLDGALGATNAVVTPYYDSMLVKMTVFARTYQQALDRMDRGLREFRIRGVKTNIPF
                     LMNVVHHPDFKTGQATTRFIDNNPDLLKFTARKDRATKLLNYLADTIVNGNPFAKGHK
                     PEKPFTAPPIPKYDHRIAPVKGTKDLLTEMGPEKFCKDWVARQKRLLLTDTTMRDAHQ
                     SLLATRMRTFDMLAVADAVARRTPNLFSLEMWGGATFDVSMRFLREDPWERLRQIRAK
                     VPNILLQMLFRGSNAVGYTNYPDNVVKGFIKHAAQNGMDIFRIFDSLNYLPNLKAAME
                     AVREDTTSICEGTLCYTGDILDPKRDKYDLQYYVRLAKELEKMGAHMLCIKDMAGLVR
                     PFAAKKLVKALKDEVGLPIHFHTHDTSGLNASSYLEASLAGVDVVDAAISSLSGGTSQ
                     PNLNSLVAAMQHTPRDTGLDPEALQEFSDYWAAVRAFYKPFDTAEPYGTAEVYLHEMP
                     GGQYTNLKEQAIGMGLGPRWPEIAHAYAEVNQLCGDIVKVTPSSKVVGDLAIECVARG
                     VKPADIFNLTGTKWSKDVTSMFEGWLGEPFYGMEANKAADSRKKWNKLADAIVGKDGK
                     RIKGRPGTHAAKINLADVRAELKGKLKKDPTEDDVWSYLMYPDVFLKFADFRKAHGDV
                     SVLPTPAYYYGLRDKEEIHISLEEGKTLFVRLLNMTEPDQNGQQTAIFELNGYPRHTT
                     VTNKALAKNAVTKAKADPADSTQVGAPMPGMVASIAVSVGQKVKEGETLLTLEAMKMF
                     AAVASPTAGTVIEICVKIGESVESKDLLVRLVK"
     gene            10696..11490
                     /locus_tag="PLP58_10695"
     CDS             10696..11490
                     /locus_tag="PLP58_10695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009960272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L family deacetylase"
                     /protein_id="HRJ09029.1"
                     /translation="MPSALALFAHPDDIEFVAAGTLLLLQEKGWDLHYMNLCTGNGGS
                     VEMDGPTTARTRLAEAREAARILGATFYPPICDDLEVTYSTPLLRKVAAVVRQARPSI
                     VLTHSPADYMEDHQNTQRLACTGAFAHAIENFVTDPPEPSFLHDVTVYHAMPHGLCDP
                     LRKRIHAGSYVNTTRVHARKRESLAAHVSQKNWLDVSQGMDSYLVSMDEASRKVGRLS
                     GQFEYAEGWRRHLHLGFSGSEIDPLKDALGADCLINEAYEAWLQQP"
     gene            complement(11491..11982)
                     /gene="lspA"
                     /locus_tag="PLP58_10700"
     CDS             complement(11491..11982)
                     /gene="lspA"
                     /locus_tag="PLP58_10700"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: protein motif:HMM:TIGR00077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HRJ09030.1"
                     /translation="MIRLLLLLSLPLYILDQWTKLWIVREFRLHEDMREVIPGIFWLH
                     HTANTGVAFGMFNGTEYANYAFGAISLGALAFIIWIYRKGAFPGWMSRAAVALLVAGI
                     FGNLTDRLMHGYVVDFLLFDFGFPPFNPWPSFNVADSCVVSAAILLALASFTEAKLAA
                     KMP"
     gene            12011..12931
                     /locus_tag="PLP58_10705"
     CDS             12011..12931
                     /locus_tag="PLP58_10705"
                     /inference="COORDINATES: protein motif:HMM:NF025198.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4190 domain-containing protein"
                     /protein_id="HRJ09031.1"
                     /translation="MAASSTGRLDKAEGSGLNPAESSPAIMHFHITKDGQQFGPLPVD
                     EIRQRIAAGYFSEDDLAWTEGMADWRPLREALPAPLAPPPIYQTGAKPAQILPSKPSA
                     TAAARTSGLAVASLVCGLIGIVIPLVPALLAVIFGHIARSSIKRSAGALSGAGMALAG
                     VIIGYLMLFVVVLAIAASLLVPAFSVMTEKAVQIKSTNNARQLVLGMKLFAADNNGAY
                     PPDLETLFKEGYLEDRRLLECIVDRNGNQVGWDYRGAGLSDTTAGNTLVLVSGGESRA
                     GVMIAAFADSSAQVLKAEKLRLIEEQRGPP"
     gene            complement(12941..14890)
                     /gene="asnB"
                     /locus_tag="PLP58_10710"
     CDS             complement(12941..14890)
                     /gene="asnB"
                     /locus_tag="PLP58_10710"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR01536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HRJ09032.1"
                     /translation="MCGIAGFFTLPGSRPAEQMTDEVRRMTDAIISRGPDDAGAWVDA
                     GAGIALGHRRLSILDLSPLGHQPMTSACGRFVIVFNGEIYNYQDLRAELEAHGHAWRG
                     HSDTEVMLAAFAQWGVLEAARRFNGMFAFALWDRQERVLHLGRDRLGEKPLFYGRVGG
                     TLVFGSELKALRRFPGFDAAVDRASVCALLRYSYVPDPHCIYEGFHKLPPASLLSLRL
                     PDEEAKPRLWWSLREVIERGQTAPFTGTETEAMDAFESLLKRAVGQRMVADVPLGAFL
                     SGGVDSSLIVAMMQAQSTRPVKTFTIGFDVPEYNEAVFAADVARHLRTDHTEMYVTAK
                     DALDVIPLLPALYDEPFSDYSQIPTYLVCKMARQHVTVALSGDAGDELFGGYERYFVG
                     RNLWNKFAWMPAPMKRALAGLMTLPPPALLNQAGDLLKPLLPKRVRHMPFGDKLHKLA
                     EVVAAPGMETLYLTLMSHWKQPEQVVIGGHERATSITDRAGWPHVADFTHRMMHLDME
                     NYLPGDILTKVDRAAMGVSLEGRIPLLDADLIEFAWSIPFSMKVREGRGKWLMRETLY
                     RHVPKPLIDRPKRGFGVPLEHWLRADLREWAEDLLSEERLKREGFFHPAPIRQKWREH
                     LQGTRNWNFYLWDVLMFQAWLESVR"
     gene            complement(14900..17287)
                     /locus_tag="PLP58_10715"
     CDS             complement(14900..17287)
                     /locus_tag="PLP58_10715"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HRJ09033.1"
                     /translation="MPAAPPARHLVFLIRDLGRGGAQRQLTVLASSLAKRAGWEVTVV
                     HFYPGEFEAELRASGVRCVCVNKRRRWDMAGFFLRLRRVLRELRPDVLHGYLHEANLA
                     ALMMKPFCGWPLVVWGIRDSRTDADTWGWLGRLSFRLNCLCSGLADLIVANSQAGRDY
                     YLGHGYPADRFFVVPNGIKAPTDAAPPAENQGFRFGLIGRLHPAKDHATFLRAAAEAR
                     RSHPQARFVCVGGGTDTYAERMRALSAELGLEDCLEWLPATGDMAAVYGGLGAVVSAS
                     EHEGFSNVLAEAMAHGLPCIASAVGDSAFLLGDGEWTFTARDAPALAAKMRVLMDLDS
                     ESRAALGARLRERVRAEFSVERLVERTETLLGGAPVAEKSRILWITTGLGTGGAEMML
                     TQLVTRLDDHSHTVLSLTAGGKHVETLRAAGIEVHSLDMPAGRPTAGALLQLAALVRA
                     RRPALIMGWMYHGCLAALMARSLGGRRVPVVWNIRQSLYDLALEKRGSALVIRLLAKL
                     SALADVITYNSETSARQHEALGYEEAKTRLIPNGFDLEKWRPLPRPRAAGSKLRVGRF
                     GRYTAMKDYPAFVEAAALVARQMPDVEFLLAGPGVDAGNAELVECVTRHFGAAAGAVR
                     LLGERHDLPELTASLDLAVSSSAFGEGFPNVIGEAMACGVPVVATDIGDTRWVMGGAA
                     AVGALAKARETGHAHETDPRILANAPTETGVLSSLDTRGNASQCNPNSEPQTSNFEFR
                     ISPFLAASMLRILSLPEAERRALGLAGRARIEAHFSLDSVLRQYEELFSTLTFHD"
     gene            17531..>18844
                     /locus_tag="PLP58_10720"
     CDS             17531..>18844
                     /locus_tag="PLP58_10720"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HRJ09034.1"
                     /translation="MDSPAPDGKMNAPPASAPVTSHTMPVTGEADFDQTIKIKTPGAG
                     MMVFGRYKLTRVLGRGGMGVVWLADDIKLERPVALKFLPSLIGLDPAAVKELKTETRR
                     GLELSHPNIVRIYDFVDDEEAAAISMEYVDGKTLSELRLGTEHGVFSAQEISKWLLGV
                     CDALDYAHFQRKVVHRDLKPANIMFAEKGSVAKLADFGIARSLSDTMSRLSAAHTGSS
                     GTLPYMSPQQAMGERPLPTDDVYSLGATIYELLTGKPPFYRGDLPTQIHGKVPPRMAE
                     RREELEVKAPDAIPDVWETVIAMCLHKDPAMRPANAGQLAELLGLKPASSTSRAYPRI
                     PPPSAAKPEKKNLLPLALAGAGVVIAGAAYWMLSGGGEKTVPVVAAAAAANEPAASPA
                     PEKEAAPPPVQPSPAETPPSEAATAAVTQPAEPAPAPDALPAAMPA"
BASE COUNT         3428 a         6050 c         5899 g         3467 t
ORIGIN      
        1 gcccagcccg gcgcggtctt ccgccagctc ggcgccggcg cggtgctcta cctggccagc
       61 gacgacctct ggcgctggcg cttccaggtg gccgacctct atcaccagcg cctgtggatg
      121 cagctcgccg catggattgc cgcgccgccc ttccaggcgg aaaacgaccg cgtctccatc
      181 ggcgcggacc gcctgcgcca tctgccggga gagaccgccg aaatccgcgt ccgcctgcgc
      241 gatgccgatg gcgcgctcat ttccagcgcc cagccgcgcg ccatcctcat ccatgagggg
      301 cgggaaatcg ccacactgac gcttgaaccc gatcccacgc actacggcat ctaccgcgcc
      361 accaccccgc cgctgaaagc gggcgcctac gaagtggccg tgtccgaagg ccccgccgcc
      421 gcgcgcgccg aaccgcgcct ctccctgcgc gtggccgaca ccggcagcac cgagtgggcc
      481 agcctgacca tgaaccgccc gctgcttgag gcggtggcct ccgcgtccgg cgggcgcttc
      541 ctgcgcgagg agcaggccgc caccgacctg ccgaacctgc tccaggccat agaccgccgc
      601 cagaccaccg tgcgcgagac aaacctgtgg tcgagctggt ggtggttcgg cgcggtgatc
      661 tgcctgctca ccgccgagtg gctgctgcgc aaaaagctgc ggcttgtctg accctccaca
      721 ggcgaaaccc gcgccacctt ccctgttgac agtctcattg aatcaggaaa gtgacactca
      781 gccggttcaa ccagccgcac atgccatgat cgaaggaatc caagccaccg tcagcgccga
      841 ggaactgcgt gaactctgcc tcaaacacgc gcgccagcac gaggccagcg tgcagcagtt
      901 gacccgcgag ctggaaggcg gcaagggccg caaaaagaag accaggaccc gccgcgagat
      961 ggaggacgac accctcaacc tcatcggcta ccactcgaac aaggcgctgg agatgaagtt
     1021 cttcgccgac catctggagc cgggggaaaa atacatgctc gatcgcaacg acctctgcca
     1081 gctcggcatc atccaggagg accgggagtt caaccgcgga tggtcggacc ccttcgaaga
     1141 cgagattccc ttttgacacc gccgcggcag ccagccggac ggcgcgcgct cagcgccgct
     1201 gccgcgccgc ctcataaagc gtgatggcca cggcggtcga aaggttcagg ctgcgcgctc
     1261 caggcctcat ggggatgtgc cacgacgcgc ccggatgccg ctcaagaatg gacggcggga
     1321 tgccgcgcgt ctccggcccg taaaccaggc acagctcgcc cgctccggaa aaatccgcct
     1381 cccagtgcgg ccgctccccc tggcactcga tgaaggccat ccgcgcccct ccatgctccc
     1441 gctcgaagtt ctcccacccc tcccacacgc gcacatccac ttgctgccag tagtccagcc
     1501 cgctgcgttt gagatacttg tcctccaggc tgaagccgag aggcttgatc aaatgcagcc
     1561 gcgcccccgt tgccaggcac agcctgccca ccgcccccgt gttgtgcggg atttccggct
     1621 ggtagaggac gacatggagc atgggagagg cctagaccgg acttcggact tcgaaactcc
     1681 gagtcgaagt cgaaactcga ttctgggtgt cgaagtacga gtctggattt cggattttca
     1741 gcccccggtc ttgaggtcct ggtacatctg gtagccctgc gcgggcgtca gcccctcatg
     1801 caccacgccg cgcaccgcct gcatcatcgc caccggcgcg tcgctctgga agatgttccg
     1861 gcccatgtcc accccgctgg ctccctgctg gatggcgttg tggcacatcg tcaaggcgtc
     1921 gagctccggc agcttcttgc cccccgcgat cacgatgggc accgggcagc aggaggtgat
     1981 ggtgtcaaag ccctcgtcgg tgtagtagca cttcaccaca ttggccccca actccgccat
     2041 gatgcgcgtg gccaggcgga aatactgcgc gttgcgcgcc atcgcccggc ccacggccac
     2101 cacgcccatc accccgatgc cgtagcggct cgccgcgtcc accaggtcgg tgaggttttt
     2161 caacgactgc cgctcatacg cgctgccgat gaagacctgc acggccaaaa ccgaggcgtt
     2221 gagccggatg gcctcctcga tgctcagcgc ggtgctttca ttggacagcg gctcgaagcc
     2281 ggggcgcccg agcttgaact tcttgtcgcc gatctcgccc tcccactcct caaaggggct
     2341 gaccatgctc gtgccgcccg aggcgcgcag ggcgatggcc ttctgcgtgc tcgccgggat
     2401 gatgctgcgc tggatgccgc gcgtgagcat gaggcagtcc gcatacggct ccaggggcag
     2461 gatggtctgg tccacgcgct ccaggcccgt ggtggggccc tggaagtagc cgtggtcgaa
     2521 ggccagcatg acggtgcgcc cgtccctggg attgaagacc ctggagagcc tgtttttcag
     2581 gccccagtca tactggtggg agcctttgag gaagaagccg ggggcttcct gggggacgtg
     2641 ggtgaaaaat tccttctcct tcttgtctgc tgcgctggtg ctgaggtcgg ccatgatgtg
     2701 cggtgggttg aatattgagc cgccacattg gacggctcac ccgcccgcgc aagccgcgct
     2761 ttggcgcacc ctgttacagc gcattgatgc ggaatgcgct gccgtgaaaa agagcagtgt
     2821 ctttccccgg cgcatgaacc gtctgtccgg tcaccgatga ctcagtcctg caagaaagcc
     2881 aggcagccgg gccgccctcg aactgccgtc ccacaaatcg agtttgcggt cccgcagatt
     2941 gaatctgcca ccgtgcgaaa gcgctttggg ggccgctgaa aacgatgcgt ggccgcgggg
     3001 actttcgggt gacgaaaccc cggctgtacc gtgattgcaa cctgcccagg cccctttact
     3061 tctctttcct catgactctc cgcgaacctg aaatcgtcga actgaccacg cccaccggac
     3121 cgatgcgcac gatggtgctg aggccggcgg cggcggggcg ctatccgggg ttgttgtttc
     3181 actcggagat ttttcagatc acggggccga ttcatcgcac ggcggcgttt ttggcggggc
     3241 atggcttcgt cgtcgcgctg ccggagattt accatgagta tctgccggcg ggcacggtgc
     3301 tcgcgtatga tcaggcgggc tcggatgtgg gcaatcgctt gaagacggag aagcccgtgg
     3361 cggcctacga cgcggacaat cgcgcagtgc tggacttctt gaagcagcac aaggcctgca
     3421 ccggccgcct cggctccttc ggaccgtgca tcggcggcca tctcacctac cgcgccgcct
     3481 tgcagccgga tgtcggcgct gcgacctgct tttatccgac ggatctgcac aagcgcagcc
     3541 ttggcgcggg catgaatgac gactcgctcg cccgcgcggc ggggattcgc ggcgagttga
     3601 tgcttgtgtt tggccgccag gaccccatgt gcccgccgaa ggccgcgaac tcatccacca
     3661 agccctcacc accgccggcg tgaacttcac ctggcacgag gtcaatgccg cccacgcctt
     3721 cctccgcgat gaaggcctcc gctacgacgc cgaactcgcc cgccagatgc tcggcatgac
     3781 ggtggatttc ttccgcaggg tgctggccgc gtgaggcagc ctcagtccca cacatgcttc
     3841 tcatcccact cgatgcggtg ggcttggagg aagcggcggt attcgtcttg gaaggtgtgt
     3901 ttggcgtggt gctcttcctg gttggcgatg taggcgcgca cgtcgtcggt tttggattcg
     3961 ctgacggaga agatgccgta gccggactgc cagtggaact tggagagcat cggatcgcga
     4021 tgggcctgat cgatgagcca gacggtggag gcgcgtttca tttccttcac ccagtccgcc
     4081 accgtgatcg tgcgggcgag gcgcaggagg atgtgaacgt gatccgccac gccgccgacg
     4141 atgagcgtag ggcagtcgag tcgcgccgag atgccgccaa ggtagcgatg gagttcgtcg
     4201 cgcagcggtt tgtcggtgag gaaggcaaaa cggtccttcg tggaccacac cgcgtgcagg
     4261 aggatgtttg ccagggattg cggcatggtg aaaaggttag cgatagcccg gaggattcgt
     4321 caatccgcca gtttgttgca tcgaaccgca acgcggttcc gtcatgcagc ccaggagtgg
     4381 acgcgcagcg gcctctcctg ggaaaacgag gcccccaaac gcctccgcat cgcaccggac
     4441 cccaaagggg tcccgtcatg cagcccaagg gtgccgagcc ttggcgaggc tcccttgggt
     4501 tcgcgggcca catcgtcccc atcccgttcc cggaccgcaa cgcggtcccg tcaggcggcg
     4561 atgcgtggcc gcggggacgt tcgggtgacg gaaccccggt ggggttcgtg atcggggatc
     4621 ggaggtggta tgtgggcgca ggtcccagga gagcactcgc catcgctcgg cattcctggg
     4681 ctgcatgagg caagcccgtt gggcttgtgg gagttacaca ctgtaaaaat ggcaccctta
     4741 agatgaggca ttttctcctt tagcgcgctg ggcaacaaca aagtcaatca agcggtgaag
     4801 aactggcgtt cccccacgga aaataattcg gctgcgatac ttttcgaccc ccagccttag
     4861 cgacagtttc ccaaacactt cgtcagccac tgaaggcatg atgtccacga ttccatcgag
     4921 atcgacctca attacattgg catcaagaag cgtggtcgcc aattcggcac gaatagccaa
     4981 gccgatttca cgagtgagca gctcggttcc aaactgtaga agcggaaatg atgtctttga
     5041 aggagcaggc tgaactgaag ccgtttccac aagtgcgact gttcctactt gagggggcgt
     5101 ttgaggttca gggattattt tagagacctc ttcctcgtaa acgtcttcca agggcaatgg
     5161 attggcgaga tctagcagca gtgagataat tgttccggac caaggagttt ggagtttctt
     5221 cttttcggtc ttgaaccgat tgactgttac aaactcatcg ccgctaacca cagttaaatg
     5281 acctctgttc aactcgatga ggcgtttaat cgtaaaaaga ccgtatccga aatgcgagct
     5341 gccaccaggg gtgatctcac ggggtttgga agagtttaat ccatcaaggg caatctcaac
     5401 agcggaagca ttggccttga gcaatcgctg cttcacttct tcatttgtcc cttcaaggta
     5461 gctgtttcgt attccaatgc cgctgtcagc aattgttatt tcgcatttgt cgccatgctg
     5521 tgacctatgg acctttgcgt gtagatagcc cggagtggta cttccagaat gcaccaaaac
     5581 gttctcacac aattccgtga ggattacccg aaaggcggag atgtcccgca ggtagccagc
     5641 ccattcatcc accattttgt atatcttgtt taccgtgctt tcaaccgagt gcccccttac
     5701 gatcggctgc aaaggaacgt ttccagtctt tgaatacacc aaagcaggcg cttctgtgtt
     5761 ctgctcaacg atgtcaacaa tgcccacatt ttgcaaaagc cgcttggcgg cttcttcgag
     5821 tgaattgtca ttcatttcca cctcgactcc gtcgggaagt gatttgcgcc acgctgcgat
     5881 aatcggaaaa acacacgaat agagatatgc attgcccaac ttgatcgtga tctttccttt
     5941 aatggacttt ggagatggga actgagtgag cagcgagtgt atcccgtcgg gattggtcgt
     6001 ttgaaggaga ttaatagtgt gggccatgat gagaatcggt tttaacagtg tgtaaacaag
     6061 ccggcaagtt gcgaagttaa gagttcttct tcgttacttc acttcaccaa cctcaccagc
     6121 aagtccttgc tctcgacgct ttcgccgatt ttgacgcaga cttcgatgac ggtgccggca
     6181 ctcgatgagg cgacggcggc gaacattttc atgggtattg ggtgggactc acgtatgagc
     6241 tttaaggcgg cgaggtactt tggcgcggct tcgaatggcc gggatgctgg attggctgcg
     6301 aaaccaggcc atgcgctcgg ccatggtcat gcctgcggtc gtttcggcga cagtctgctt
     6361 ccattcgcgg ctcgcggcca cggcatcgaa ggcagcggtt tcagtctggc tgttcttggt
     6421 cggcataaat gatctccttg gggctgacaa tacggacgat gggccagccg cgcaagatgt
     6481 tcactgcgtt gaatccatcc tcacgacgca cattgacaag gtgcttgaag ttccaactca
     6541 cgatgaggcg gacgccgtga accgtcgcca tggcgacgtg cagggcatcg tcggcaaact
     6601 tgggcggcac gacggcagcc tgcacgtagg cttcggcaag ttcttcaacc tcatggctgg
     6661 tgtcgagaat gttagacgca tcaaaagtgt cggcgaaaat ttgccgcaca ttcgccgggg
     6721 cattctggat ttcagcctcg gcaacgatgg aggtgatcag cgaccaatgt ccggcctttg
     6781 cctgctgcca gagcagccgc gtgtcagcca tccagagcgg atcgtgataa ccaccgacga
     6841 ccgaggtgtc gatgtagagc ttgcgcgctg gatcgggcat cggtggcggt gattgtgggt
     6901 taatgggcta cttcaccagc ctcaccagca gatccttgct ctccacgctt tcgccgattt
     6961 tgacgcagat ctcgatgacg gtgccggcgg tgggggaggc gacggcggcg aacattttca
     7021 tggcttcgag ggtgagcaga gtttcgcctt ccttgacctt ttgaccgacg ctgacggcga
     7081 tgctggcgac catgccgggc atgggggcgc cgacttgggt gctgtcggcg ggatcggcct
     7141 tggctttggt gacggcgttt ttggcgaggg ctttgttggt gacggtggtg tggcgcgggt
     7201 agccgttgag ttcgaagatg gcggtctgct ggccgttttg gtccggctcg gtcatgttga
     7261 ggaggcggac gaagagcgtt ttgccctcct cgaggctgat gtggatctcc tccttgtcgc
     7321 gcaggccgta gtagtaggcg ggcgtgggca gcacggagac atcgccatgg gccttgcgga
     7381 agtcggcgaa cttcaggaag acatcgggat acatgaggta gctccagaca tcgtcctcgg
     7441 tggggtcctt cttcaatttg cccttcagct cggcgcggac gtcggcgagg ttgatcttcg
     7501 cggcgtgggt gccggggcgg cctttgatgc gtttgccgtc cttgccgacg atggcgtcgg
     7561 cgagcttgtt ccacttcttg cggctgtcgg cggccttgtt ggcctccatg ccgtagaaag
     7621 gttcgccgag ccagccttcg aacatgctgg tgacatcctt gctccacttg gtgccggtga
     7681 ggttgaagat gtcggcgggc ttcacaccac gcgcgacgca ctcgatggcg aggtcgccga
     7741 cgactttgct cgatggcgtg accttgacga tgtcgccgca gagctggttc acctcggcgt
     7801 aagcgtgggc gatctccggc cagcgcgggc cgaggcccat gccgatggct tgctctttga
     7861 gattcgtgta ctggccgccg ggcatctcgt ggagataaac ctcggcggtg ccgtagggct
     7921 cggcggtgtc gaagggttta taaaacgcac gcacggcagc ccagtagtcg ctgaactcct
     7981 gcaacgcctc gggatcgagg ccggtgtcgc gcggcgtgtg ctgcatggcg gcgacgagcg
     8041 agttcaaatt cggctgcgag gtgccgccgg agagcgagga gatggccgca tcgacgacat
     8101 cgacgccagc gagcgaggcc tcgaggtagc tcgatgcatt caggccgctg gtgtcatgcg
     8161 tgtggaagtg aatcggcagg ccgacttcat ccttcagtgc cttcacgagc ttcttcgcgg
     8221 caaaggggcg gacgagtccg gccatgtctt tgatgcagag catgtgtgct cccatctttt
     8281 cgagctcctt ggcgaggcgg acgtagtatt ggaggtcata cttgtcgcgc ttcggatcga
     8341 ggatgtcgcc ggtgtagcag agcgtgcctt cgcagatgga tgtggtgtcc tcgcgcaccg
     8401 cttccatcgc ggccttgagg tttggcagat agttgagcga gtcaaagatg cggaagatgt
     8461 ccatgccgtt ctgcgcggca tgtttgatga agcctttgac gacattgtcc gggtagttcg
     8521 tgtagccgac ggcgttcgag ccgcggaaga gcatctgcaa aaggatgttc ggcacctttg
     8581 cgcggatctg gcgcaggcgc tcccacgggt cctcgcgcag gaagcgcatg ctcacgtcaa
     8641 acgtggcgcc gccccacatt tcgagcgaga agaggttcgg cgtgcggcga gcgaccgcat
     8701 ccgcgacggc gagcatgtca aaggtgcgca tgcgcgtggc gagcagcgac tgatgcgcgt
     8761 cgcgcatcgt ggtgtcggtg agcaacaggc gcttttgtct ggcgacccag tctttgcaga
     8821 acttctccgg ccccatctcg gtgagcaggt ccttcgtgcc cttcacgggc gcgatgcggt
     8881 gatcgtattt cggaatcggc ggcgcggtga agggcttctc gggcttgtgg cccttggcga
     8941 aggggttgcc attgacgatg gtgtccgcga ggtagttcag cagcttggtg gcgcggtcct
     9001 tgcgggcggt gaacttgagc aggtcggggt tgttatcaat gaagcgggtg gtggcctggc
     9061 cggtcttgaa gtccgggtga tgcaccacgt tcatgaggaa ggggatgttc gtcttcacgc
     9121 cgcggatgcg gaactcgcgc agaccgcggt ccatgcggtc gagcgcctgc tggtaggtgc
     9181 gggcaaagac ggtcatcttc accagcatgg agtcgtagta gggcgtgacg acggcgttcg
     9241 tggcgcccag cgcaccgtcc aggcgcacgc cgaagccgcc ggggctgcgg tagttcagga
     9301 tgcggccaaa gtcgggcgtg aaggcgtttt cggggtcctc ggtggtgatg cggcactgga
     9361 tggcgaagcc gcttttctcg atcttctcct gcggcggcag gccgagcggc tcctggtgca
     9421 tctgatagcc ctgcgcgatg aggatctgcg agcggacgat gtcgatgctg gtgatctcct
     9481 ccgtcacggt gtgctccacc tggatgcgcg gattcatctc aatgaaatac cactcgcccg
     9541 tctcgtgatc gacgaggaac tcgaccgtgc cggcgtgcgt gtacttcact tcctccgcca
     9601 gcttcaccgc cgcgtcgcaa agaccgtcaa tgatctcctg cttcacgcca tagctcggcg
     9661 cctgctcgat caccttctgg tgacgacgct gcaccgagca gtcacgctca tgcagatgca
     9721 gcaccgtgcc gtgtttatcg gccaaaattt gcacttcgat gtgcttcgcc ttgccgacga
     9781 atttttccaa aaacaccgcg ccattgccga aagcacgttc cgcctcggtt tgcgcctcat
     9841 cgagcagctt ttcgaggtct ttggcctcgc gcacgatgcg catgccgcga ccaccgccac
     9901 cgaaggccgc cttgatgatc agcggaaagc cgattttctt cgccacggca atcgcttcct
     9961 tgcgatcact caccggctcg tccgtgcctt cgaggatcgg cacgttcaat ttgcgagcga
    10021 cattccgcgc cgccgttttg tcacccatca tcgtcaaaat cttcggatca ggcccgatga
    10081 agatgatgcc cgccttttcg caggccttcg agaagtccgg attctcgctg agaaagccgt
    10141 agccggggtg gatggcgtcc acgccctttt ccttcgccag cgtcacgatg ccttcgacat
    10201 cgaggtaagc gccgagcggc cctttgtcct tgttcagctc ataggcctcg tccgccttga
    10261 agcggtgcgg gcagaagcgg tcctcctgcg cgtagatgcc gacggtgcgg atgcccagct
    10321 ccgtggccgc gcgcatgacg cggatggcga tttcggagcg gttcgccacc atgagcttct
    10381 tgatcgggcg gatcgcaggc ggggcggagg cgctgacggc ggacttggcg gcggatttct
    10441 tgggcatgtg aggttgggat gaaaaagacg ggctgatttt caatggcgga cggccttttg
    10501 caaggtgggg atgatagagg cgggaagttc ggggagattc ttttgctgca agggcgtcag
    10561 tatgggggac aggcgtcccg cctgttcgca caggcaagat gcccgtgccc catattcaag
    10621 cccgtgcccc atgctctgag ttttcggagc gttcttgaca agtccggcgc attcccggac
    10681 aaaagcgccc ccgccatgcc ctccgccctc gccctcttcg cccaccctga tgacatcgaa
    10741 ttcgtcgccg caggcaccct gctgctgctc caggaaaagg gctgggacct gcattacatg
    10801 aacctctgca cgggcaatgg cggctcggtg gaaatggacg gccccaccac agcgcgcacg
    10861 cgcctggccg aggcgcggga ggccgcgcgc attctcggcg ccacgtttta tccgccgatc
    10921 tgcgatgacc tggaggtgac ttacagcacg cccctgctgc gcaaggtggc cgctgtggtg
    10981 cggcaggcgc gcccgagcat cgtgctcaca cactcgcccg ccgactacat ggaggaccat
    11041 caaaacacgc agcgcctggc ctgcacaggt gcctttgccc acgccatcga aaacttcgtc
    11101 accgacccgc ccgagccctc ctttttgcat gatgtcaccg tctatcacgc catgccgcac
    11161 ggcctgtgtg acccgctgcg caagcgcatc cacgccggct cctatgtgaa caccacgcgc
    11221 gtccatgcca ggaagcgcga gtctttggcc gcgcatgtca gccagaaaaa ctggctcgat
    11281 gtcagccagg gcatggactc ctacctcgtg agcatggacg aggcatcgcg caaggtgggg
    11341 cggctttccg gccagttcga gtatgccgag ggctggcgca ggcatctgca cctgggcttc
    11401 agcggcagcg agattgaccc gctcaaagac gccctgggtg ccgactgcct catcaacgaa
    11461 gcctacgagg cctggcttca gcagccatga tcacggcatc ttcgccgcca gcttcgcctc
    11521 ggtgaaagag gcgagcgcga gaagaatggc ggcggagacc acgcaggagt ccgccacatt
    11581 gaaggacggc cagggattga agggcgggaa accgaagtcg aagagcagga agtccaccac
    11641 atagccgtgc atgagtcggt cggtgagatt gccgaagatg cccgccacaa gcagcgccac
    11701 cgccgcgcgg ctcatccagc cggggaaggc gcccttccgg taaatccata tgatgaaagc
    11761 cagcgcgccg agcgagatgg cgccaaaggc gtaattggcg tattccgtgc cattgaacat
    11821 gccgaaggcc acgccggtgt tcgccgtgtg gtgcagccag aagatgccgg gaatgacctc
    11881 gcgcatgtcc tcatgcaggc ggaactcgcg cacgatccag agcttcgtcc actggtcgag
    11941 aatgtagagc ggcaggctga ggagaagcag gaggcgtatc atcggagggg cgatccaggt
    12001 agccggaaaa gtggccgcat caagcacagg caggctcgac aaggcggaag gcagcggctt
    12061 aaatcccgcc gaatcctctc ccgccatcat gcacttccac atcaccaaag acggccagca
    12121 gttcggtccc ctgcctgtgg acgaaatccg ccagcgcatc gccgcaggct acttctccga
    12181 ggacgacctg gcctggaccg agggcatggc cgactggcgt ccgctgcgcg aggctctgcc
    12241 cgcccccctg gctccgccgc ccatttatca aaccggcgcc aagcccgcgc agatcctccc
    12301 ctcgaagccg tccgcaaccg ccgccgcccg cacttccggt ctggccgtgg cgagcctcgt
    12361 ctgcggcttg atcggcatcg tcatcccgct ggttccggcc ctgcttgcgg tcattttcgg
    12421 ccacatcgcc cgctcctcca tcaagcgctc cgcaggcgcg ctgagcggcg cgggcatggc
    12481 gctggcgggc gtcattatag gctatttgat gctgttcgtc gtggtcctgg ccatcgccgc
    12541 ctcgctgttg gtgcccgctt tttccgtcat gacggaaaag gctgtccaga tcaaaagcac
    12601 caacaacgcc cgccagctcg tgctggggat gaagctgttc gccgccgaca acaacggcgc
    12661 ctatccgccc gacctggaga cacttttcaa agaaggctat cttgaggacc gccgtttgct
    12721 ggaatgcatc gtggatcgca acggcaatca agtcggctgg gactaccggg gtgcgggcct
    12781 gagcgacacc acagcgggga acaccctcgt cctggtcagc ggcggcgagt cgcgcgccgg
    12841 cgtgatgatc gccgccttcg ccgacagctc cgcgcaggtg ctcaaggcgg agaaactgcg
    12901 cctgatcgag gaacagcgcg gcccgccttg aggctgaccc tcacctaacc gactccagcc
    12961 aggcctggaa catcagcaca tcccagagat agaagttcca gttgcgggtg ccttgcaggt
    13021 gctcgcgcca tttctggcgg atgggggcgg ggtggaagaa gccttccctt ttcaggcgct
    13081 cctcgctgag caggtcttcg gcccattcgc gcaggtcggc gcgcagccag tgctccaggg
    13141 gcacgccgaa gccgcgcttg gggcggtcta tgagcggctt gggcacatgg cggtagagcg
    13201 tctcgcgcat gagccatttg ccgcgtccct cgcgcacttt catggaaaag gggatgctcc
    13261 aggcgaactc gatgaggtcc gcgtccagca gagggatgcg cccctccaag ctgacgccca
    13321 tggcggcgcg gtccactttg gtgaggatat cgcccggcag gtagttctcc atgtccaggt
    13381 gcatcatgcg gtgggtgaag tccgccacat gcggccagcc cgcgcggtcg gtgatgctgg
    13441 tggcgcgctc atgaccgccg atgaccacct gctccggctg cttccagtgg gacatgagcg
    13501 tgaggtaaag cgtctccatg cccggcgcgg ccaccacttc ggccaacttg tgcagcttgt
    13561 cgccaaaggg catgtggcgc acgcgcttcg gcagcagcgg cttcaggaga tcgcccgctt
    13621 gattgagcaa agccggcggc ggcagcgtca tgagcccggc cagggcgcgc ttcattggcg
    13681 cgggcatcca ggcgaatttg ttccaaagat tccgccccac gaagtagcgc tcgtaacctc
    13741 cgaaaagctc gtcgcccgcg tcgccggaca aggccacggt gacatgctgg cgcgccattt
    13801 tgcagacgag gtaggtgggg atttgcgagt agtcggagaa aggctcgtca tacagcgcgg
    13861 gcagcagcgg gatgacatcc agcgcgtcct tggccgtgac atacatctcc gtgtggtcgg
    13921 tgcgcagatg ccgcgccaca tcggcggcga agacggcctc gttgtactcc ggcacgtcga
    13981 agccgatggt gaaggtcttc accgggcgcg tgctctgcgc ctgcatcatc gccacgatga
    14041 gggaggagtc cacaccaccg gagaggaagg cgcccagcgg cacatccgcc accatgcgct
    14101 ggcccacggc gcgcttgagc aggctctcaa aggcgtccat ggcctcagtc tccgtgccag
    14161 tgaagggcgc ggtctgcccg cgctcgatca cctcccgcag cgaccaccac aggcgcggct
    14221 ttgcctcctc atcaggcagg cgcaggctga gcaacgaggc gggcggcagc ttgtgaaagc
    14281 cctcgtaaat gcagtgcggg tccggcacat aactgtacct gagcagcgcg cacaccgagg
    14341 cgcgatccac cgccgcatcg aagcctggaa aacgccgcag cgccttcagc tccgagccga
    14401 agaccagcgt gccgcccacg cggccgtaaa agagcggctt ctcgccgaga cgatcccgcc
    14461 ccaggtgcag cacgcgctcc tgccggtccc agagggcgaa ggcgaacatg ccgttgaagc
    14521 ggcgcgcggc ctccagcaca ccccattgcg caaaggcggc gagcatcacc tccgtgtcgg
    14581 agtgcccgcg ccacgcatgg ccgtgcgcct ccaactccgc gcgcaggtcc tggtagttgt
    14641 agatctcgcc attgaagacg atgacaaagc ggccgcaggc gctggtcatc ggctgatgcc
    14701 cgagcggcga gagatcgaga atggagagcc gccggtgtcc gagcgcgatg cccgcccccg
    14761 cgtccaccca cgcgcccgcg tcatccggcc cgcggctgat gatggcgtcg gtcatgcgcc
    14821 ggacctcgtc cgtcatttgc tccgccggac gcgagcctgg cagggtgaag aatccagcga
    14881 tgccgcacat gggggaagat cagtcgtgaa aagtgagtgt ggagaaaagc tcctcatact
    14941 gccgcagcac ggagtcgagc gagaaatgcg cctcgatgcg cgcgcgtccc gccagaccga
    15001 gggcgcggcg ttcggcttcg gggagggaaa ggatgcgcag catggaggcg gcgaggaagg
    15061 gcgaaattcg aaattcgaaa ttcgaagttt gaggctcgga gtttgggttg cattgggatg
    15121 cgtttccccg agtgtccagc gaactgagga cacccgtctc cgtaggcgca ttcgccagaa
    15181 tgcggggatc ggtttcatgc gcgtgaccgg tctcccgcgc tttggcaagc gcgcctacgg
    15241 cagccgcccc ccccatcacc cagcgcgtgt cgccgatgtc ggttgccacc accgggacgc
    15301 cgcacgccat cgcctcgcca atgacgttgg gaaagccctc gccgaacgcg gaggatgaca
    15361 cggcgaggtc aagcgatgcg gtgagttccg gcagatcgtg gcgctccccg agcagacgca
    15421 ccgcgcccgc cgccgcgccg aagtggcggg tgacgcattc cacgagttcg gcattgcccg
    15481 catccacacc cggcccggcc agaaggaact ccacgtccgg catctgccgc gccacgagcg
    15541 ccgccgcctc gacaaatgcg gggtagtcct tcatcgccgt gtaacgcccg aaacgtccca
    15601 cgcgcagctt gctcccggcg gctcgcggtc gcggcaaagg ccgccatttt tccagatcga
    15661 acccgttcgg gatcagccgc gtctttgcct cctcgtaacc cagcgcctca tgctggcgcg
    15721 cgctggtctc cgagttgtaa gtgatgacat ccgccagcgc ggacagcttt gccagcaggc
    15781 ggatcaccag cgccgagccg cgtttttcca aggcgagatc gtagagcgac tggcggatgt
    15841 tccagaccac gggcacgcgc cgtccgccca gactgcgcgc catcagcgcc gcgagacagc
    15901 cgtgatacat ccagcccatg atcaaagccg gacgccgcgc gcgcacgagc gccgccagtt
    15961 gcagcaaagc ccccgccgtg ggccgccctg cgggcatgtc caggctatgg acctcgatgc
    16021 ccgcggcgcg cagtgtctcc acatgcttgc cgccagccgt gagggaaagc acggtgtggc
    16081 tgtgatcgtc gagccgtgtc acgagttgcg tcagcatcat ttccgcgccg ccggtgccga
    16141 ggcccgtggt gatccagaga atgcgcgact tttccgccac cggcgcgccg ccgagaaggg
    16201 tctcggtgcg ctccaccagc cgctccacgc taaactccgc ccgcacccgc tcccgcaacc
    16261 gcgcgcctag cgccgcgcgt gactcgctgt ccaggtccat caagacgcgc atctttgccg
    16321 ccagcgctgg agcatccctg gcggtgaagg tccactcccc atcgcccagc aaaaaggccg
    16381 agtctcccac cgcgctggcg atgcagggca ggccgtgtgc catcgcctcc gccagcacat
    16441 tggaaaagcc ctcgtgctcc gaggcggaca ccaccgcgcc aagcccgcca tacaccgccg
    16501 ccatgtcgcc ggtggccggc agccattcca gacagtcctc caggccgagt tcggcggaca
    16561 aggcgcgcat ccgctccgca taggtgtccg tgccgccgcc cacgcacacg aagcgcgcct
    16621 gcggatgcga gcgccgcgcc tccgccgccg cgcgcagaaa ggtggcgtgg tccttggccg
    16681 gatgcaggcg gccgatgagg ccgaagcgga acccttgatt ctcagccggg ggcgctgcat
    16741 ccgttggtgc cttgatcccg ttcggcacca caaaaaagcg gtccgccggg taaccgtggc
    16801 cgaggtagta gtcgcggcca gcctgcgaat tcgccacgat gaggtcggcc agccctgagc
    16861 aaaggcagtt caggcggaag ctcaaccgcc ccagccagcc ccaggtgtcc gcgtccgtgc
    16921 gcgagtcgcg aatgccccag accacgagcg gccacccgca gaagggcttc atcatcagcg
    16981 cggccagatt cgcctcatgc aaatagccgt gcagcacatc cgggcgcagc tcccgcagca
    17041 cgcgccgcag ccggaggaaa aagcccgcca tgtcccaccg gcggcgcttg ttcacgcaca
    17101 cgcagcgcac cccggaggcg cgcagttccg cttcaaattc gcccggataa aaatgcacca
    17161 ccgtcacctc ccacccggcc cgctttgcca gggaggatgc cagcaccgtc aactgccgct
    17221 gcgcccctcc gcgaccgagg tcgcggatga ggaaaacgag gtgtcttgca gggggggcgg
    17281 ctggcaccgg gaacgataat cgccataaca acaccctggc aatcagggaa tcttggagct
    17341 cgtcagactg tcccaaactc cgtctgaccc gtccgcacat ccgtctgact ggacctcaag
    17401 gcgcacctcg tatgaaaatc accgcacaaa ctgtggcaga cgggccttca tcctgctccc
    17461 ggcttcgcag aaattatccg gtgacagcgc cggaggaaga aactaccatc ccgccgtttt
    17521 cccccgaatc atggacagcc ccgccccaga tggaaagatg aacgcgccgc ctgcctccgc
    17581 gccggtgacc tcgcacacca tgcccgttac gggggaggcg gattttgacc agaccatcaa
    17641 gatcaagact cccggcgcgg gcatgatggt ctttggccgc tacaaactca cccgcgtgct
    17701 ggggcgcggc ggcatgggcg tggtgtggct ggcggatgac atcaagctgg agcggcccgt
    17761 ggccttgaaa ttcctcccca gcctcatcgg cctcgatccc gccgcggtga aggaactcaa
    17821 aaccgagacc cgccgcggcc tcgagctttc ccacccgaac atcgtccgca tttacgactt
    17881 cgtggatgac gaggaggccg cggccatctc catggaatat gtggatggca aaacgctctc
    17941 cgaactgcgc ctgggcaccg agcacggcgt cttcagcgcg caggaaatct cgaagtggct
    18001 gctcggtgtc tgcgacgcgc tcgactacgc ccactttcag cgcaaagtcg tccaccgcga
    18061 cctcaagcca gcgaacatca tgttcgctga aaaaggcagc gtggccaagc tggccgactt
    18121 cggcatcgcc cgcagcctct ccgacaccat gagccgcctc tccgccgcgc acaccggcag
    18181 cagcggcacc ctgccctaca tgagcccgca gcaggccatg ggggagcgcc ccctgcccac
    18241 ggacgatgtt tactccctcg gcgccacgat ttacgagctg ctcaccggca agccgccctt
    18301 ttaccggggc gacctgccca cccagattca cggcaaggtg cccccgcgca tggccgagcg
    18361 ccgcgaggaa ctcgaggtca aagctcccga tgccatccct gatgtgtggg aaaccgtcat
    18421 cgccatgtgc ctgcacaaag accctgcgat gcgtccggcc aacgcgggcc agctcgccga
    18481 attgctcggc ctcaagcccg cctccagcac cagcagagcc tacccgcgca tccccccgcc
    18541 gtccgccgcc aagcctgaaa agaaaaactt gctgccgctc gccctggccg gagccggtgt
    18601 cgtcatcgca ggagccgcct attggatgct ctccgggggc ggggaaaaaa ccgtgcctgt
    18661 cgtggcagcg gctgccgccg ccaatgagcc ggctgcttct cccgcccctg aaaaagaagc
    18721 cgctcctcca ccggtgcagc cgtcacctgc ggagacacca ccgtccgaag ccgccaccgc
    18781 agccgtcacg cagcctgcgg agcctgcccc cgcgcctgac gcccttccgg cagccatgcc
    18841 cgcc
//
LOCUS       DAOMYJ010000086        18184 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Prosthecobacter sp. isolate AMR_MDS_5629
            NODE_1055_length_18184_cov_21.048210, whole genome shotgun
            sequence.
ACCESSION   DAOMYJ010000086 DAOMYJ010000000
VERSION     DAOMYJ010000086.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867850
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prosthecobacter sp. (wastewater metagenome)
  ORGANISM  Prosthecobacter sp.
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Verrucomicrobiaceae; Prosthecobacter.
REFERENCE   1  (bases 1 to 18184)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 18184)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.30
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:34:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,742
            CDSs (total)                      :: 4,684
            Genes (coding)                    :: 4,676
            CDSs (with protein)               :: 4,676
            Genes (RNA)                       :: 58
            tRNAs                             :: 55
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18184
                     /organism="Prosthecobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1055_length_18184_cov_21.048210"
                     /isolate="AMR_MDS_5629"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1965333"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(302..745)
                     /locus_tag="PLP58_10725"
     CDS             complement(302..745)
                     /locus_tag="PLP58_10725"
                     /inference="COORDINATES: protein motif:HMM:NF012272.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="globin domain-containing protein"
                     /protein_id="HRJ09035.1"
                     /translation="MNPHQVHLIRKSFAELGRHEHLAALVFYKRLFEIAPELRPMFKG
                     DIEAQSKKLLDMLGVLIAMLERPLGLELELKAMGARHADYGVKDGHYAIVGAALLDML
                     QTTLDKDFTPETRAAWTALYGAVEMFMKAGAKEASEEHASHVAGD"
     gene            816..1472
                     /locus_tag="PLP58_10730"
     CDS             816..1472
                     /locus_tag="PLP58_10730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007415192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HRJ09036.1"
                     /translation="MPSSTMQKKILIIEDQAPMRRNLALMLELEGYQVCVAENGRAGV
                     EAARKSRPDLILCDVMMPEMDGYGVVQALREDDSFANTPFIFLTAKSERADVRMGMNF
                     GADDYLTKPVVRDDLLAAVQTRLDRAEALQQRITDNQGFSPDFERPDLLQRAYGLTGR
                     EAEVLLWVAQGKSNSDVSAILEMSEKTVKQHLGSCFQKMGVESRSAAAVMALEALNTK
                     "
     gene            complement(1517..3016)
                     /locus_tag="PLP58_10735"
     CDS             complement(1517..3016)
                     /locus_tag="PLP58_10735"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HRJ09037.1"
                     /translation="MFLIPILSTGATPNVIIIQTDEHNFRTLGCYRALLPPDQAFIWG
                     EGVKVDTPHIDSIAARGAVCARYYATSPVCTPSRAALMTGMYPQNTGSMSNDLPMLDT
                     METFAAVLAANGYETGYAGKWHLDGDAKPGWAPERRFGFADNRFMFNRGHWKKLGMDE
                     NGPKVLSVDDRGRPVYSAEGADEKSFTTDFLMDRVIEFVRQKRAAPYCFMVSIPDPHG
                     PNTVRAPYDTMFDPSAFKQPQSALAKGARLPSFAQVLEDRFNQRQMAQYFGMVRCIDD
                     NVGRLITALRETGQFDNTMLIFTSDHGDMCGEHGRHNKGIPCEGSARIPFVIAAPGII
                     PPGTVIHQALGTVDFKPTLLGLLGLKTNRVMQGRDASALFKDGGAPEGWKDRGFVRIG
                     GGEKAAGGWVAVFTRRHKLVLSPTDDPALFDLDSDPFEMTNLFHDSARRGVIRDLANE
                     LKNYLATSGDPHAKSAPVMADISWAVSPAAEYSPPAREKARRARAERED"
     gene            complement(3139..5163)
                     /locus_tag="PLP58_10740"
     CDS             complement(3139..5163)
                     /locus_tag="PLP58_10740"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase"
                     /protein_id="HRJ09038.1"
                     /translation="MLRSVLLLFALCPSLSASSSQPNIIVILADDLGWSDTTLYGTTT
                     FYQTPNVERLAKRGMTFMRAYSASPLCSPTRSALLTGLSPARTGITTPNCHEPRVVLE
                     TTMPKTAAPNQKAIQPSPPTRLKTEYRTLAETLKGAGYATAHFGKWHLGSEPYSPLQQ
                     GFDFDLPHHPGPGPAGSFVAPWKFANFKEKSPGEHIEDRMAAEAVTWMEQQKGKPFYL
                     NYWMFSVHAPFDAKKANIEKHRGRIDPKDAQRSPTYAAMIQSMDDAIGTLLDALDRLR
                     IADNTIIIFTSDNGGNMYNEVDGTTPTSNRPLRGGKATMFEGGTRVPCVVVWPGIAEP
                     GSRSDAIIQSEDFYPTLMESLQSSHLAPRDEPSALLAKKTSDSSKAFSDSGSPQLVSR
                     SETATFDGHSILPALKGDALAGKAVFQYFPHAPGVPDWLPPAVSVHRDDWKLIRIFHG
                     GEKGAHRHLLFNLRDDLGEKNNLAAQKPELVAELDALIETFLTETKAVVPVPNPAFDP
                     AKYHPELEGIQSNKKPAKTSTATKGKARANTMLRSGWQASQHARLSAKNGALVITSTG
                     GDPYISTRDLPQDMRGPLIFTLRLSSTGAGGGLLYYCDSPGQTFHKERTVAFPIIHDG
                     QTHDQGLTLPVERLTALRLDPGNAPGTIVIEHLELRDASGLSVGLPALTP"
     gene            complement(5259..5900)
                     /locus_tag="PLP58_10745"
     CDS             complement(5259..5900)
                     /locus_tag="PLP58_10745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ09039.1"
                     /translation="MFVSASADEHKRLGEPRNCYLYSWGKGPNWITSITTEFRLSLLA
                     REMPRVDDQYFEPTDKALIWVRKENEPVITTLASVDGKKVIQVVYPEQGGFGKTIGTI
                     LVAIETSRDSGWYSPFFAAQPELYRGQFVSGKDVKFGYVATLEWSGTGSMRSHYLFDL
                     QAPHPKIVATCDAGRVRPTDYKNETEYAQALKVFDREAELLSGTIPATQGDRK"
     gene            complement(5963..7753)
                     /locus_tag="PLP58_10750"
     CDS             complement(5963..7753)
                     /locus_tag="PLP58_10750"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HRJ09040.1"
                     /translation="MKSFRLLFTLMVMASGLPLLAQPAKPNLIVFYTDDHGWSDLGIL
                     GIQKDLKTPHLDALARSGVVARSGYSTAPQCVPSRAGLLAGRFQARFGVESNQSPLDG
                     FNEQTTFAKRLQDAGYATAQFGKWHLGPAHEITRHGFTHVFSQNSQRPFAANITPDGQ
                     DRPMSDLPPAAYHIDACSQAAAAVIDRHKDQPFFLYIAYRAPHTPLDAPQRYKDRFPG
                     EMPERRRAALGMIAAMDDGVGLITGTLKKHGLTEKTLIFFIGDNGAPLKIHKVDSPLD
                     GDAGGWDGSLNDPLNGEKGMLSEGGMRVPFVIAWPGTIPGGQVYEHPVSALDVAATAV
                     ASIGNRSSEDSITNRIYGDLDGVNLLPYLTGENKAPPHNALYWRWMAQSAIREGNWKL
                     LRGGDREYLYDLANDREEKHNLAAKHPEIATRLRAKLKTWADGLNPPGMALGPMAATW
                     NDYFDHYLEGKTITPQAEKPAPAASATDGWEARNGKLTAKDGLFILTPENSAKPTFIT
                     KAKLKLAGPVTAQITLKTTATGQAALAWRLDGDKDFLPANRLPFPLQSTPDWQTHTLN
                     LPTTARIIHLRVHFPGAAEIREIELKAGKR"
     gene            7943..10963
                     /locus_tag="PLP58_10755"
     CDS             7943..10963
                     /locus_tag="PLP58_10755"
                     /inference="COORDINATES: protein motif:HMM:NF019223.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PSD1 and planctomycete cytochrome C
                     domain-containing protein"
                     /protein_id="HRJ09041.1"
                     /translation="MPRASAALSATGVALAFGMASCAFAAQPVDFGKDVLPVLSDKCF
                     HCHGPDASSRKAKLRLDLEDEVRKPRSDGTELLVPGDAAASLLIQRVTSTDPDEVMPP
                     PDFVKKLSSAEVTALTAWVEQGAKWEAHWAHQPVRRPALPPGFEHRHPVDAFIDAALA
                     KAGLTPNPRADAATLQRRAALDLTGLPPSDESDLSDLSDFIQKLLRSPHYGERMAWDW
                     LDLARYADTHGYQKDNLRSMWRWRDWVVEAFNRNLPFDQFTILQLAGDLLPQAGVEQI
                     LPTGFNRNHRLNAEAGAIDEEYRVEYVLDRVETTATAWMGLTAGCARCHDHKYDPLSQ
                     REFFALAAFFNNLDERGNDGVGLVAEPAMTLPVAGMERAVAEAESAVEKAVVALEAAA
                     LSADLDAWGAMQKQRLETGGLWRVLEPLQALGQDKGSTFTPMADGSILFGGANPLNDV
                     HEARLTVRDGFTLHQLRLEALPDASLTGGSLARSFDGAFLLSGLDVEIHGTAQKLRAF
                     QATHAADESPLHHATDADPLTGWRVPGGLRERVRAVFELAQPVQVRAGSEIVIRLRYE
                     SREEQFIIGRFRLSAGAAGLKMDADEIPQSLAEAVRLGDAESLRAAYRETAPNLAPLR
                     AARDAARATLADAKAAATTQVMVMRERPGEPRATHVLKRGLYDQPGERVEPDVPAFLG
                     LPLSDDAPRDRLALARWLVDPRHPLTARVAVNRLWQMLFGAGLVRTPEDFGFQGGRPT
                     HPELLDWLAAEFLEKGWDIQAMLRLLMTSEAYARSAAATPAMLERDPENLLLARATRF
                     RLPAPMLRDQALAVSGLLVPRIGGPPVKPYQPPGLWEAVAGVNSNTVTYIQDHDENLR
                     RRSLYTLWKRSVPPPNMMLFDAAAREVCSASRPRTNSPLQALVTLNDTTYAEAARVLA
                     GRVLSEAKDDAARISRLWRIVLARPRAPQESAHLTALLQRQLARYQADPGLAKSRVSI
                     GESPLPAHLDPVELAAWTELAEVMLNLDATLTRP"
     gene            10960..12408
                     /locus_tag="PLP58_10760"
     CDS             10960..12408
                     /locus_tag="PLP58_10760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007417956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1501 domain-containing protein"
                     /protein_id="HRJ09042.1"
                     /translation="MNHALHRRVFLQQAAGLGIGATALQTLLHGEQRGKGHGGQHGEQ
                     ASRQFTHHPPKARRIIYLCQSGAPSQMDLFDPKPAMAKRFNENLPESIRKGQRLTTMT
                     SGQTRFPVAPSIFKFSRHGRSGMEISELLPHTGSIADHICLIRSMHTEAINHDPAITF
                     LQTGFEQAGRPSLGAWLSYGLGSANADLPAFVVMLSRGSGRPNCQPLYDRLWGSGFLP
                     SRHQGVKFLATGDPVLFLQNPPGSRTEARRENLDTLAALNHLEHERLGDPETLTRIAQ
                     YEMAFRMQTSVPEVTDLSDEPESTFHLYGEQARQRGTYAANCLLARRLIEKGVRFVQL
                     YHMGWDQHNALPKQIRGQCLDTDQPSAALVKDLHQRGLLEDTLVIWAGEFGRTIYSQG
                     ELTADNYGRDHHPRCFSLWLAGAGIKPGHIHGRTDDFCYNIEADPVHIHDLNATLLHL
                     LGLDHERLTWRYQGRDFRLTDVHGHLVKGIMS"
     gene            complement(12575..13957)
                     /locus_tag="PLP58_10765"
     CDS             complement(12575..13957)
                     /locus_tag="PLP58_10765"
                     /inference="COORDINATES: protein motif:HMM:NF013621.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003951 - NAD+ kinase activity [Evidence
                     IEA];
                     GO_process: GO:0006741 - NADP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+)/NADH kinase"
                     /protein_id="HRJ09043.1"
                     /translation="MPKRIAIFGGSFNPPGLHHRAIVEKLAAAFDEVRVIPCGPRPDK
                     PGTDSIPPVYRATLADLTFGGLERVVVDLSDLEQSAFSRNHQLQTRFEPDGEVWHVVG
                     ADLVAGGGDGRSAIQLSWEKGAWLWQNAAFAVLARPGFTLDPGDLPPRCQVFDLAETA
                     SSTLIREKLAQGEGVFSLLHPRAHSYIRRYGLYRAPNPGAWARGSLEGLPFSIQCAAE
                     NAKAQSWLERLPAGRSLDTADFITVLGGDGTMLRAIREHWRLRRPFFGINAGHLGFLM
                     NAPEEVLARSFPPGDVILRQLPMLYLEVTGRDGKVSTARGFNDAWLERSTSQSAWLEV
                     RVNGIPRIPKLISDGALVATAAGSTAYARSMGASPLLADTPAWLLVGSNVMEPVHWRS
                     ALLSVDSTVEIHNLEPVQRPVQAYVDGLSLGEIVSMRARISRAAAAEVVFCASHDMAE
                     KIAAIQFHRG"
     gene            complement(14036..16384)
                     /gene="lptD"
                     /locus_tag="PLP58_10770"
     CDS             complement(14036..16384)
                     /gene="lptD"
                     /locus_tag="PLP58_10770"
                     /inference="COORDINATES: protein motif:HMM:NF016348.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0010033 - response to organic substance
                     [Evidence IEA];
                     GO_process: GO:0061024 - membrane organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS assembly protein LptD"
                     /protein_id="HRJ09044.1"
                     /translation="MISSRLRAALVLASLLMAAGARGQAFFDSLTSNLNIEGMETTYD
                     PDTGLAIAKGDVSISYADVEIRCDHASYNAATGEVIARDNVVIWQAGTTYRGDNILYN
                     VNSGELSGDAVRSSFPMDTGSFFYQSEKFETETKFIDRVEGGTTYLTTHDLADPNFRV
                     RARSFTVYPGDRIVMRKVTVFAGDTPVFYLPYLSQPLTDEVGYRFTPGYQSRWGAFLL
                     NQYGVLHGDHTLAKYRLDLRSARGVGAGADFLSLRHRENRDNFGTLTLYYMYDTDADK
                     NRTSVARNPVGEDRYRVNFQHRIYLPGPQKSTWYLDFDINKISDAHFYEDFYFNDFRE
                     TPEPDNQISLVHTDPRYVATLMARFQANDFYDVGTKLPELSVDFIRRPLWSTGLYHQG
                     SLSAGIYKDELGNPEEDFLHAELLRIRTLVPPGNTSFVTAQLEGAGFGRLHTYQELLY
                     PKTFFGWLNVVPRVGAGVTHYSDIDGSVSGLDNETKTLFHAGLDVSFKVSKNWSDLSK
                     PEWGLDGLRHVIQPYLNLSYLDASQPENFPSIDRLSPTTRPRSIDPQLFTAVDDLRSW
                     NIARLGVRNLLQTRRDYTNQARGVFTSATSEPYQTYSWAGLNTYVDFFMRDPEFDRSI
                     SNLYNELFFRPVPWVNFWLDAQLPIQGETGSFTELNQGVTFMPGRNMQISLGHQYVSN
                     SPFFRDSSLVFSRIYSRLTDNWGFGMNHIFEMDDGVMEFQSYSITRDLSSWMASIGGM
                     VRDNRGGLKEYGILFSLTLKDFPQATIPLDIDPNPSGRGGSQ"
     gene            complement(16508..17293)
                     /gene="lpxA"
                     /locus_tag="PLP58_10775"
     CDS             complement(16508..17293)
                     /gene="lpxA"
                     /locus_tag="PLP58_10775"
                     /EC_number="2.3.1.129"
                     /inference="COORDINATES: protein motif:HMM:NF003657.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008780 -
                     acyl-[acyl-carrier-protein]-UDP-N-acetylglucosamine
                     O-acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-ACP--UDP-N-acetylglucosamine
                     O-acyltransferase"
                     /protein_id="HRJ09045.1"
                     /translation="MIHPTAIVHAAARLAEDVEVGPYAVIDGPAEIGPGCRIEAHAQL
                     AGRVVLGAGTVVGRGAVIGGEPQDTGYDPATESSVVIGEKNIIREHVTIHRGSKPGSV
                     TRVGDGNFIMVGAHLGHDVELGDGNILANACLLAGHVRVGSRTFIGGGAVFHQFIRIG
                     DSCVVQGNGSFGKDIPHFCAAMRTNRVTGLNVVGLRRQGFTAAERAEIKEMFRLLFCS
                     GRNITQAVSLARGREWSGPSLRMLEFVEHPGRRGVCAWRGGED"
     gene            complement(17364..>18184)
                     /locus_tag="PLP58_10780"
     CDS             complement(17364..>18184)
                     /locus_tag="PLP58_10780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009957934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HRJ09046.1"
                     /translation="LLREQAWAAGGAGGLRHSVCKWCYKDIPLEAMCAAAKEIGIESV
                     ELLDPPDFPVLKKHGLHCAMVSFPTAQGPDGKKIGSIPHGFNRVEHHDLLVQAYEPLM
                     KTSAEAGFKNIICFSGNRDGMGDEQGLENCAAGLRRLLPLAEKLGVTLTMELLNSKVN
                     HKDYMCDRSAWGAALCEKLGSEHFKLLYDIYHMQIMEGDVIATIRARHAFFSHYHTGG
                     VPGRAEIDGTQELHYPAIVKAIRETGYKGFLGQEFIPKQPDKLASLRQAVEICS"
BASE COUNT         3295 a         5642 c         5848 g         3399 t
ORIGIN      
        1 gagcgcgatg cgcgcccgat cgtgaaggcg cactgcacgc attgccatgg cgaggaggaa
       61 atacccgaag ggggtgcgga tccgcgcccg cggccaacga ggacgtggca gtcatggtgg
      121 cgagcgtggc gaggagccgg cggcgattca ttcaaggcag tcggattcga tgcggcggtc
      181 ctttcaatga ggaggatccg gtcagcacac gaagattggc tgactttccg cgcatcgtct
      241 caccggatga cttccgccat gacagcgatg gttccgcacg ccagggttcc aggccgcccg
      301 ctcaatcacc cgcgacatgg gaggcgtgct cctccgaggc ttccttcgcg ccagccttca
      361 tgaacatctc gaccgcgcca tatagcgcgg tccaggctgc acgcgtctcg ggggtgaagt
      421 ctttgtcgag tgtcgtctgc aacatgtcca gaagcgccgc gccaacgatg gcatagtgtc
      481 cgtccttgac gccgtagtcc gcgtggcggg cgcccatggc cttgagttcg agttccagcc
      541 ccagcgggcg ctccagcatg gcgatgagca cgcccagcat gtcgagcagc tttttggact
      601 gcgcctcgat gtcgcccttg aacatgggcc gcagctccgg ggcgatctca aacaggcgct
      661 tgtaaaacac cagggccgcc aaatgctcgt ggcgtccgag ttctgcgaaa gatttccgga
      721 tgagatggac ttgatgcggg ttcattggag gcagtaaaac acatcccggc cttgaaggac
      781 atccgacttt agtcggatgg tgttttgaac aagacatgcc atcatccacc atgcagaaaa
      841 aaatcctcat catcgaagac caggcgccca tgcgccggaa cctggcgctc atgctcgaac
      901 tggaaggcta ccaggtttgc gtggcggaaa acggccgcgc cggggtcgaa gccgcgcgca
      961 aatcccggcc cgatctcatt ctctgcgatg tcatgatgcc ggagatggac ggctacggcg
     1021 tcgtccaggc actgcgcgag gacgacagct ttgccaacac tcccttcatt tttctcacgg
     1081 caaaaagcga gcgggccgac gtgcgcatgg gcatgaattt cggcgctgac gactacctca
     1141 ccaagccggt ggttcgcgac gacctccttg cagcggtgca aacacggctc gaccgcgctg
     1201 aggcgctcca gcagcgcatc accgacaacc agggtttcag ccccgacttc gagcggcccg
     1261 acctgcttca gcgcgcttac ggactgaccg gccgcgaggc ggaggtcctc ctctgggtgg
     1321 cgcagggcaa aagcaattcc gatgtgtccg ccattctcga aatgagcgaa aagaccgtca
     1381 aacaacacct tggctcctgc tttcaaaaaa tgggggttga aagccgaagc gcggccgctg
     1441 tgatggcctt ggaggcgttg aacaccaagt gatcatcaag cgctcccgcg cgcgcgctgc
     1501 gcccgatgac tccgtcctaa tcctcgcgct ccgcccttgc ccggcgcgcc ttttcacgcg
     1561 cgggcggaga gtattccgca gcgggtgaga cggcccagga aatgtccgcc atgactgggg
     1621 cggacttggc atgcgggtcg ccgctcgtgg cgaggtagtt tttcagttcg ttcgcgagat
     1681 cacggatgac gccgcgccgc gcggaatcgt gaaacaggtt cgtcatttca aaggggtcgc
     1741 tgtcgaggtc gaagagcgcg ggatcatccg tgggcgagag caccagcttg tggcggcggg
     1801 tgaacacagc cacccagccg ccggcagcct tttcaccacc gccgatgcgc acgaaacccc
     1861 ggtctttcca cccctcgggc gcgcctccat ccttgaacag ggccgaggcg tcgcggccct
     1921 gcatcacgcg gtttgtcttg aggcccagca ggccgagcag cgtgggtttg aaatccaccg
     1981 tgcccagcgc ctgatggatg accgtgccag gcgggatgat gcccggagcg gcgatgacaa
     2041 aaggaatgcg cgcgctgccc tcgcagggga tgcctttgtt gtgccggcca tgctcgccgc
     2101 acatgtcgcc gtggtcggag gtgaaaatga gcatggtgtt gtcgaactgg ccggtctccc
     2161 tcagcgcggt gatgaggcgg ccgacattgt catctatgca cctcaccatg ccgaaatact
     2221 gcgccatctg gcgctggttg aagcggtcct ccagcacctg ggcgaaggag ggcagccgcg
     2281 cgcctttcgc gagcgcggac tgcggctgct tgaaggcaga ggggtcaaac atcgtgtcgt
     2341 aaggcgcgcg cacggtgttc ggcccgtgcg ggtcggggat gctgaccatg aagcagtatg
     2401 gagcggcgcg tttctggcgc acaaactcga tcacgcggtc catcaggaag tcggtggtga
     2461 aggacttctc atccgcgccc tcggcgctgt aaaccgggcg gccccggtca tccacggaga
     2521 gcaccttggg gccgttttca tccatgccga gctttttcca atggccgcgg ttgaacatga
     2581 agcggttgtc cgcgaagcca aacctgcgct ccggcgccca gcctggcttc gcgtcaccgt
     2641 cgaggtgcca cttgcccgcg tagcctgttt cgtaaccatt cgcagcgagc acggcggcaa
     2701 aggtctccat cgtgtcgagc atgggcaggt cattgctcat gctgccggtg ttttgcggat
     2761 acattcccgt catcaatgcc gcgcgggagg gcgtgcagac cggtgatgtg gcgtagtagc
     2821 gcgcgcagac cgcgccgcgc gccgcgatgg agtctatgtg cggcgtgtcc accttcactc
     2881 cttctcccca gatgaaggcc tggtcaggcg gcagcagcgc gcggtagcag ccgagcgtgc
     2941 ggaagttgtg ctcatcggtc tggatgatga tgacatttgg cgtggctccc gtgctgagaa
     3001 tagggatgag aaacaatact gacctgcaaa gactagtgat tcgactcatg ccttctgaac
     3061 gcgcgatgtc ttttgatgtt gccgctgcct tgtcacgaca ccgtgaatac ggttatcaat
     3121 gggtttggaa acgcgaactc agggcgtgag cgcgggcagg ccaacactca gtcccgaggc
     3181 atctcgaagt tcgaggtgtt cgatgacgat ggtgccggga gcgttgccgg gatccagacg
     3241 cagtgcggta agcctttcaa ccggcaaagt gaggccttgg tcgtgggtct gcccgtcgtg
     3301 gatgatgggg aaagcgacag tgcgctcttt atggaaggtc tggccgggag aatcgcagta
     3361 atagagcagg ccgccgcctg ccccagtgct gctgaggcgc agtgtaaaaa tgagcgggcc
     3421 gcgcatgtcc tgtggcagat cacgggttga gatataggga tctccaccgg tagaggtgat
     3481 gacgagtgcg ccgttcttcg cacttagcct ggcatgctga ctcgcctgcc atccggagcg
     3541 aagcatggta tttgccctgg cctttccttt cgtggctgtt gaagttttgg caggcttttt
     3601 gttgctctgt atgccttcga gttcgggatg atacttcgcc ggatcaaacg ccgggttcgg
     3661 cacgggcacg acggccttgg tctcggtgag gaaggtttcg attaaagcat cgagttcggc
     3721 caccagttcg ggcttctgcg cggcaaggtt gttcttttcg ccaagatcgt cgcgcaggtt
     3781 gaagagcagg tgacgatgcg cgcctttttc gccgccgtgg aagatgcgga tgagcttcca
     3841 gtcatcgcga tgcacggaaa cagctggcgg cagccagtcc ggcacgccag gcgcatgcgg
     3901 gaagtattga aacaccgcct tccccgccag cgcatcgccc ttcagcgcgg gaaggatgct
     3961 gtggccgtcg aaagtagccg tctcgctccg cgagacgagc tgaggactcc cgctgtcaga
     4021 gaatgctttc gatgaatcgg atgttttctt cgcgagcaaa gcgcttggct catctcgcgg
     4081 agcgagatgg ctactttgca aactctccat caacgtcgga taaaaatctt cgctctggat
     4141 gatggcatcg ctgcggctgc cgggttccgc gatgcccggc cacacgacca cgcaaggcac
     4201 gcgcgtgccg ccttcaaaca tcgtcgcctt gccaccgcgc agcgggcggt tgctggtggg
     4261 cgtggtgcca tcgacttcgt tgtacatgtt gccgccgttg tcggaggtga agatgatgat
     4321 cgtgttgtcc gcgatgcgga gccggtcgag cgcgtcgagc agcgtgccga tggcgtcgtc
     4381 catgctctga atcatcgccg cgtaggtggg gctgcgctgc gcgtccttcg ggtcgatgcg
     4441 accgcgatgc ttctcgatgt ttgccttctt cgcatcaaag ggcgcatgca cgctgaacat
     4501 ccagtagttc aggtaaaacg gctttccttt ctgctgctcc atccacgtca ccgcttccgc
     4561 cgccatgcga tcctcgatgt gctcgccggg cgacttctct ttgaagttcg caaacttcca
     4621 tggcgcgaca aaactgcccg caggccccgg ccccggatga tgcggcagat cgaaatcaaa
     4681 gccctgctgg agcggcgaat acggctccga tccgaggtgc cacttcccaa agtgcgccgt
     4741 ggcatagccc gcgcccttca gcgtctccgc cagcgtgcga tactccgtct tcaaccgcgt
     4801 gggcgggctc ggctggatgg ctttttggtt cggagcggcc gttttcggca tcgtggtctc
     4861 caaaacgacc cgcggctcgt ggcaattcgg cgtcgtgatg cccgtgcgtg ccggactcag
     4921 ccccgtgagc aacgccgacc gcgtcggcga gcacagcgga ctcgccgaat acgcccgcat
     4981 gaaggtcatc ccgcgcttcg ccagccgctc gacattcggc gtctggtaga atgtcgtcgt
     5041 cccgtaaagc gtcgtgtcac tccagccgag atcgtccgcg aggatgacga tgatgttcgg
     5101 ctgcgacgag gaagcggaga gcgaagggca aagagcgaag agaaggagga cagagcgaag
     5161 catcgcgccc tgaaacgttg aaagccgggc agagattccg gcaaagcgtg tgtcggattt
     5221 ggagaaagcg cagccgctac gttttgatgt ctcctccatc attttctgtc accctgagtt
     5281 gctggaatag ttcctgacag caactcagct tctcgatcaa agactttcag ggcctgggca
     5341 tactcggttt cgttcttgta gtctgtagga cgcacgcgtc ccgcatcgca ggtcgccacg
     5401 atctttggat gtggtgcttg aagatcgaaa agataatgac tcctcatgga gccggttcct
     5461 gaccattcca gagttgcgac gtagccgaac ttcacgtcct tgccactcac gaactgccct
     5521 cggtagagtt ctggctgagc ggcaaaaaac ggagaatacc agcctgagtc tcggctcgtt
     5581 tcgatcgcga caagtatcgt tccaatcgtc ttcccgaagc ctccctgctc gggataaacc
     5641 acttgaatca ctttctttcc atcaaccgac gccagggtgg ttatgaccgg ttcgttttct
     5701 tttcgcaccc agatcaatgc tttgtcagtc ggctcgaaat actgatcatc cacgcgaggc
     5761 atctcgcggg caagaagtga tagccgaaac tcggtagtta tcgaggtgat ccaatttgga
     5821 cctttccccc aagagtagag ataacagttt cttggctcac cgagacgttt gtgttcgtct
     5881 gcactagcag agacaaacat cgagaggaaa aagacggcta ataggcggtg catagcgatg
     5941 tgcgacagac acggctcgtt gtttaacgct tccccgcctt caactcaatc tcccggatct
     6001 ccgccgcacc ggggaaatgg acgcggaggt ggatgatgcg ggcggtggtg gggaggttga
     6061 gggtgtgggt ttgccagtcg ggggtggatt gaagggggaa tgggaggcgg ttggcgggga
     6121 ggaagtcttt gtcgccatcg agtcgccagg cgagggcggc ttggccggtg gcggtggttt
     6181 tgagagtgat ttgagcagtg acgggaccgg cgagcttgag tttggctttg gtgatgaagg
     6241 tgggcttggc agagttttcg ggcgtgagga tgaaaaggcc gtctttggcg gtgagcttgc
     6301 cgttgcgggc ttcccacccg tcagtggcgg aggcggcggg cgcgggcttt tcagcctgcg
     6361 gggtgatggt tttgccttcg aggtagtgat cgaagtagtc gttccacgtc gcggccatgg
     6421 ggccgagcgc catgccgggt gggttcaggc catcggccca ggttttgagt ttcgcgcgga
     6481 gtcgcgtggc gatctccgga tgctttgcgg cgaggttgtg cttctcctcg cggtcattgg
     6541 cgaggtcgta gaggtattcg cggtcgccac cgcgcagaag tttccagttt ccttcgcgaa
     6601 tggcgctctg ggccatccag cgccagtaga gcgcgttgtg tggcggtgct ttgttttcgc
     6661 cggtgaggta ggggagaaga ttgacgccgt cgaggtcacc gtagattcga ttggtaatcg
     6721 aatcctcaga gcttcgattg ccaatcgaag ctactgccgt ggcggccaca tcgagcgcac
     6781 tcacgggatg ctcataaacc tgaccgccgg ggatggtgcc gggccaggcg atgacgaaag
     6841 gcactctcat gccgccctcg gacaacatgc ctttttcgcc attcagcggg tcgttgaggc
     6901 tgccgtccca gccgccggca tcaccgtcca gcggggaatc caccttgtga atcttcagag
     6961 gcgcgccgtt gtcgccaatg aagaagatga gcgtcttctc tgtgaggccg tgttttttca
     7021 atgtgccagt gatcaggccg actccgtcat ccatggcggc aatcatgccg agcgcggcgc
     7081 ggcgcctttc cggcatctcg ccgggaaacc ggtccttgta gcgctgcggc gcgtcgagcg
     7141 gcgtgtgcgg tgcgcggtag gcgatgtaga ggaagaacgg ctggtctttg tgacggtcaa
     7201 tcactgcggc agcggcctga gaacaggcgt caatgtgata agcggcgggg ggcagatcac
     7261 tcatggggcg gtcctggccg tcgggggtga tgttggcggc aaaggggcgc tgggagtttt
     7321 gggaaaagac atgagtgaag ccgtggcggg tgatctcatg cgcgggcccg aggtgccatt
     7381 tgccgaactg ggcggtggca tagccggcat cctggaggcg cttggcaaag gtggtctgct
     7441 cgttgaagcc atcgagcggg gactggttcg actccacgcc aaagcgcgcc tgaaaacggc
     7501 cggcgagcag cccggcgcgc gagggcacgc actgcggcgc ggtgctgtag ccgctccgcg
     7561 ccacgacgcc ggacctcgca agcgcgtcga gatgcggtgt tttgaggtct ttttgaatgc
     7621 cgaggattcc gaggtcggac caaccatggt catcggtgta aaaaacgatg aggttgggct
     7681 tggcaggttg tgccagcaga ggcaagccgg atgccatcac catcagagtg aaaagaagac
     7741 ggaacgattt catgacgcgc ctggaaacga atcgcgcacc gcagactctc ggcaaaccgt
     7801 gtgtcggatt cggcggcctg gagctggcac ccgggtttga aaatgaggca ttgagtctgt
     7861 gtttttccgt cactggcatg atgcgtctcg gattcgcgca tcctctgccg tataaatgct
     7921 ggaaatgaag gaatcccgcc agatgcctcg cgcctctgcc gcactctccg ccacgggtgt
     7981 ggcgctggcg ttcggcatgg catcctgcgc ttttgcggcg cagccggtgg actttggaaa
     8041 agacgtgctg ccggtgctct cggacaagtg tttccactgc cacgggccgg acgcctcgtc
     8101 gcggaaagcg aaactgcggc ttgatctgga agacgaagtg agaaagccgc gctcggatgg
     8161 aacggagctt ctcgtgccgg gggatgccgc cgccagcctg ctcatccagc gcgtcaccag
     8221 caccgatccc gatgaagtga tgccgccgcc ggatttcgtg aaaaaactca gctccgccga
     8281 ggtgaccgcg cttaccgcct gggtggagca gggcgcgaag tgggaggcgc attgggcgca
     8341 tcagcctgtg cgtcgtccgg cactgccgcc gggctttgag caccggcacc cggtggatgc
     8401 cttcattgac gccgcgctcg ccaaggccgg cctcacgccc aacccgcgcg cggatgccgc
     8461 caccctgcaa agacgcgctg cactggacct cacggggctg ccaccgtcgg acgagtcaga
     8521 cttgtctgac ctgtcggact tcatccagaa actcctgcgc agtccccatt acggcgagcg
     8581 catggcctgg gactggctgg acctcgcgcg ctatgccgat acgcacggct accagaagga
     8641 caacctgcgc agcatgtggc gctggcggga ctgggtggtg gaggcattca accgcaacct
     8701 gccctttgac cagttcacga tcctgcagct cgccggcgac cttttgccgc aggccggcgt
     8761 cgagcaaatc ctgcccaccg gcttcaaccg caaccaccgc ctcaatgccg aggccggcgc
     8821 cattgatgag gaataccgcg tcgagtatgt gctcgaccgc gttgagacca cggccaccgc
     8881 ctggatgggc ctcaccgcag gctgcgcgcg ctgtcatgac cacaagtatg acccgctcag
     8941 ccagcgcgag ttcttcgccc tggccgcctt tttcaacaac ctcgacgagc gcggtaatga
     9001 cggcgtcggc ctcgttgccg aacccgccat gacgctgccg gtggctggca tggagcgcgc
     9061 cgtggctgag gccgagtccg ccgtggaaaa agccgtggtg gctttggaag cagccgccct
     9121 ttccgccgac ctcgatgcct ggggcgccat gcaaaagcag cgcctcgaaa ccggcggcct
     9181 gtggcgcgtg ctggaaccgt tgcaggcgct cggtcaggac aaggggtcca ccttcacgcc
     9241 gatggcagac ggctccattt tgttcggcgg agcaaatccg ctcaacgatg tccacgaagc
     9301 gcgcctcact gtgcgtgacg gcttcacgct gcatcaactc cgcctcgaag ccctgcctga
     9361 tgccagcctc accggcggca gcctggcgcg cagtttcgac ggcgcgtttt tgctctccgg
     9421 tctggatgtg gaaatccacg gcaccgcgca aaaactgcgc gcctttcaag ccacgcacgc
     9481 cgcggatgaa agcccgctgc atcacgccac cgacgccgat ccactcaccg gctggcgtgt
     9541 cccaggcggt ctgcgcgagc gggtgcgagc tgtctttgag ctggctcagc ccgtgcaggt
     9601 gagggcgggc agcgagatcg tcatccggct gcgttacgag tcgcgcgagg agcagttcat
     9661 catcgggcgc ttccgcctca gcgcgggcgc ggcggggctg aagatggatg cggacgaaat
     9721 acctcagtct ctcgccgagg ccgtgcgtct gggcgatgcc gagtccctgc gcgccgctta
     9781 ccgcgaaacc gctcccaatc tcgccccgct gcgcgccgca cgggatgccg cgcgcgccac
     9841 gctcgctgac gcgaaagccg ccgccaccac gcaggtcatg gtcatgcggg agcgccccgg
     9901 cgagccgcgc gccacgcatg tgctgaagcg cggcctttat gaccagcccg gcgagcgcgt
     9961 ggagccggac gtgcccgcct tcctcggcct gcccctgtcg gatgacgcac cgcgcgaccg
    10021 cctcgccctc gcgcgctggc ttgtggaccc gcgccatccg ctcaccgcgc gcgtggcggt
    10081 gaaccgcctc tggcagatgc ttttcggcgc gggactcgtg cgcacgccgg aggacttcgg
    10141 ctttcaaggc gggcgcccca cgcatcctga actgctcgac tggctcgccg ccgagttctt
    10201 ggaaaaaggc tgggacatcc aggccatgct gcgcctgctc atgacctccg aagcctacgc
    10261 gcgctccgcc gcagccacgc ctgcgatgct ggagcgcgac ccggagaacc tcctcctggc
    10321 ccgagccacg cgcttccgtc tccccgcgcc catgctgcgg gatcaggcgc tcgccgtcag
    10381 cggcctgctc gtgcctcgaa tcggcggtcc gccggtcaaa ccgtatcaac cgcccggcct
    10441 ttgggaagcc gtggccggcg tcaacagcaa caccgtcaca tacattcagg atcatgatga
    10501 aaacctgcgc cggcgcagcc tttacaccct ctggaaacgc agcgtgccgc cgccaaatat
    10561 gatgctcttc gacgccgccg cgcgcgaggt ctgctccgct tcccgcccgc gcacaaactc
    10621 gcctttgcag gccctcgtca cgctcaatga caccacctat gccgaggccg cgcgcgtgct
    10681 cgcggggcgg gtgctctcgg aggcgaaaga cgatgccgcg cgcatcagtc ggctctggcg
    10741 catcgtgctc gcacgtccgc gcgcgccgca agaaagcgcg catctcaccg cgctgctcca
    10801 gcgccagctt gcgcgttatc aagccgaccc cggccttgcc aaaagccgcg tcagcatcgg
    10861 cgagtcccct ttgcccgcgc atctcgaccc cgtcgaactc gccgcctgga ccgagctggc
    10921 ggaagtgatg ctgaacctcg acgccaccct cacccggcca tgaaccacgc cctgcaccgc
    10981 cgtgttttcc tccagcaagc cgccggtctt ggcatcggtg ccaccgcctt gcaaacgctg
    11041 ttgcatgggg agcagcgcgg gaaagggcat ggggggcagc atggggaaca ggcgtcccgc
    11101 cagttcactc accacccgcc caaagccaga cgcatcatct acctctgcca gtccggcgcg
    11161 ccctcgcaga tggatctctt cgatcccaaa cctgcgatgg caaagcgctt caacgaaaac
    11221 ctgcccgagt ccatccgcaa aggccagcgc ctcaccacca tgaccagcgg ccagacccgt
    11281 ttccctgtgg cgccgagcat cttcaaattc agccgccatg gccgcagtgg catggagatc
    11341 agcgagctgc tgccgcacac cggcagcatc gccgaccata tctgcctcat ccgctccatg
    11401 cacaccgagg ccatcaatca cgacccggcc atcacctttc tccagaccgg cttcgagcag
    11461 gccggccgcc ccagcctcgg cgcctggctc agctatggcc tgggctcggc gaatgccgac
    11521 ctgcccgcct ttgtcgtcat gctctcgcgc ggcagcggac gaccgaattg ccagccgctc
    11581 tacgaccgcc tctggggcag cggctttttg ccctcgcggc atcagggagt gaaatttctc
    11641 gccacgggtg atccagtgct gtttttgcaa aacccgcccg gctcacgcac tgaggcgcgt
    11701 cgcgagaacc tcgacaccct cgccgcgctc aaccatctgg agcacgagcg actgggcgat
    11761 cccgagactc tcacgcgcat cgcccaatac gagatggcct tccgcatgca gacctccgtg
    11821 cccgaggtca ccgacctcag cgacgagccc gagagcacct tccacctcta tggtgaacag
    11881 gcgcgccagc gcggcaccta tgccgccaac tgcctgctcg cccgccgcct catcgagaaa
    11941 ggcgtgcgct ttgtgcagct ctaccacatg ggctgggacc agcacaacgc cctgcccaag
    12001 caaatccgcg gccagtgcct cgacactgac cagccaagcg ccgcgctcgt gaaggacctc
    12061 caccagcgcg gcctgctcga agacacgctc gtcatctggg ccggtgaatt tggacgcacc
    12121 atctacagcc agggcgagct gacagccgac aactacggac gcgaccacca tccgcgctgc
    12181 ttcagtctct ggctcgccgg tgcgggcatc aagcccggcc acatccacgg ccgcacggat
    12241 gacttttgct acaacatcga ggcggacccc gtgcatatcc acgatctaaa tgccacgctg
    12301 ctgcatctgc tcggcctcga ccacgagcgt ctcacctggc gctatcaagg ccgcgacttc
    12361 cgcctcaccg atgtgcatgg gcacttggtg aaggggatta tgagttgagg tgtgccgcac
    12421 cgtccgctca tcgaaaatca gttccgcctg cttgtgtggc agttgtgctg ttgtacaaaa
    12481 tcatcatccg tttttcgtcc cgcggatgag cattcaaatg tggcgttcgg gactcagggg
    12541 tggctctgcg cggcagtgaa taccgcctcc gaaatcaccc tcggtggaac tgaatggcgg
    12601 cgattttttc cgccatgtca tgactggcgc aaaagacaac ctcggcggcg gcggcgcggc
    12661 tgatacgggc gcgcatggag acgatctcgc ccagactaag tccgtccaca taggcctgca
    12721 cgggtcgctg cacgggttcg agattatgaa tctcgaccgt gctgtccacg gagagcaggg
    12781 cgctgcgcca gtggaccggc tccatgacat tcgagccgac gagcagccag gcgggcgtgt
    12841 cggccagcag cgggctggct cccatgctgc gggcgtaggc cgtggagccg gcggcggtgg
    12901 cgaccagggc gccgtcgctg atgagcttgg gaatgcgggg gatgccgttg actctgacct
    12961 ccagccaggc gctctggctg gtggagcgct ccagccatgc gtcattgaat ccgcgggcgg
    13021 tgctgacttt gccatcgcgc cctgtgacct ccaaatagag catggggagc tggcgcagaa
    13081 tgacatcccc tggtggaaaa gacctggcca gaacctcctc cggcgcgttc atgaggaatc
    13141 cgaggtggcc ggcattgatg ccaaagaaag gccgccgcag gcgccaatgc tcgcggatgg
    13201 cgcggagcat ggtgccgtcc ccgccaagca cggtgatgaa gtccgccgta tcgagggaac
    13261 ggccagcggg gaggcgctcc agccagcttt gcgctttggc attttcagcg gcgcactgga
    13321 tgctgaaggg caggccttcg agactgccgc gcgcccatgc gccgggattt ggggcgcggt
    13381 agagtccgta gcggcggatg taggaatgcg cgcgcggatg cagcaaggag aagacgccct
    13441 ccccttgcgc gagcttttcg cggatcagcg tgctggaggc ggtctcagcc agatcaaaga
    13501 cctggcagcg cgggggcagg tctccgggat ccagcgtgaa gccgggacgc gcgagcacgg
    13561 caaaggcggc gttttgccag agccacgcgc ctttctccca ggaaagctga atggcggagc
    13621 gcccgtcccc gccgccggcg acgaggtccg cgccgacgac atgccagact tcaccgtctg
    13681 gctcgaagcg cgtctgcaac tggtggttcc ggctgaaagc ggactgctcc aggtcggaca
    13741 agtccacaac gacacgctcc aagccgccga atgtcagatc ggcaagagtg gcgcgataaa
    13801 ccggcgggat ggagtccgtg ccgggcttgt cagggcgcgg gccgcaagga atgactcgca
    13861 cctcgtcaaa ggcggcagcc agtttttcca cgatggcgcg gtggtggagt ccgggcgggt
    13921 tgaagctgcc cccgaagatg gcgatgcgct tgggcatgat gggagagtgt ggctcgcgtt
    13981 ccgggcggcg gcaaggcgcg tgtgacgcgg cgcagtcagg ccgccgcgcg cggggtcact
    14041 ggctgccgcc tcgtccggag ggattcgggt caatgtcgag cggaatggtg gcctgcggga
    14101 agtctttgag cgtcaggctg aagaggatgc cgtattcctt caagccgccg cggttgtcgc
    14161 gcaccatgcc gccgatggag gccatccagc tcgaaaggtc gcgggtgatg ctgtaactct
    14221 ggaattccat gacgccgtca tccatttcaa agatgtggtt catgccgaag ccccagttgt
    14281 ccgtgaggcg gctgtagatg cgggagaaga cgaggctgga gtcgcggaag aaggggctgt
    14341 tgctcacata ctggtggccg agggagatct gcatgtttct gccgggcatg aaggtgacgc
    14401 cctggttcag ctcggtgaag ctgccggtct cgccctggat cgggagctgg gcgtcgagcc
    14461 agaagttgac ccaaggcacc gggcggaaaa agagctcgtt gtagaggttg gaaatggaac
    14521 ggtcgaactc ggggtcgcgc atgaaaaagt ccacataagt gttaagtccg gcccaggagt
    14581 aggtctggta aggctcgctg gtggcggagg tgaagacgcc gcgcgcctgg ttggtgtagt
    14641 cgcgccgggt ttgcagcaga ttgcgcacgc cgaggcgggc gatgttccag gagcgcaggt
    14701 catccacggc ggtgaagagc tgcgggtcaa tggagcgcgg gcgcgtggtg ggcgagagcc
    14761 ggtcaatgga ggggaagttt tccggctgcg aggcgtcgag ataggataag ttcaaatagg
    14821 gctggatgac gtggcgcaga ccgtcgagtc cccattcggg tttcgagagg tcggaccagt
    14881 ttttggacac tttgaaagac acgtcgaggc cggcgtgaaa gagcgtcttg gtctcgttgt
    14941 ccaggccgct gacactgccg tcaatgtccg agtaatgggt gacaccggcg ccgactctgg
    15001 gcaccacgtt cagccagccg aagaatgtct tcggatacag caattcctgg taagtgtgca
    15061 ggcgcccgaa gccggcaccc tcaagctggg cggtgacaaa actggtgttc ccgggcggga
    15121 cgagggtgcg aatgcgaagc aactcggcat ggaggaaatc ctcctccggg ttgcccagct
    15181 cgtccttgta gatgccggcg ctcagggagc cttgatggta gagaccggtg ctccacagag
    15241 ggcggcggat gaagtccacg gagagctccg gcagcttggt gcccacatca tagaagtcgt
    15301 tggcctggaa gcgggccatg agcgtggcca cataacgcgg gtcagtgtgg acgagggaga
    15361 tttgattgtc cggctcggga gtctcgcgga agtcgttgaa gtaaaagtcc tcgtagaagt
    15421 gggcgtcgct gatcttgttg atgtcgaagt ccagatacca ggtgctcttt tgcgggccgg
    15481 gcaggtagat gcggtgctgg aagttcaccc tgtagcgatc ctctccgacg ggattgcgcg
    15541 ccacgctggt gcggttctta tccgcgtcgg tgtcgtacat gtaataaagc gtcagcgtgc
    15601 cgaagttgtc gcggttttcc cggtggcgca gggagaggaa gtccgcgccc gcgccgacgc
    15661 cgcgcgcgga gcgcaggtcg aggcggtatt tggccagcgt gtggtcgcca tgcagcaccc
    15721 cgtactgatt gagaaggaag gcaccccagc ggctctggta gccgggcgtg aagcggtagc
    15781 ccacctcgtc ggtcagaggc tgcgagagat agggcaggta gaaaaccggc gtgtctccgg
    15841 cgaagacggt gacctttctc atcacaattc ggtcgccggg atagaccgtg aagctgcggg
    15901 cgcgcactcg gaagttcgga tcggcaagat cgtgcgtcgt cagataagtc gtgccgcctt
    15961 ccacgcgatc aatgaatttc gtctcggtct caaacttctc cgactggtaa aaaaagctgc
    16021 ccgtgtccat cggaaaggag ctgcgcacag cgtcaccgct cagttccccg gagttcacat
    16081 tgtacaagat gttgtccccg cggtaggtcg tgcccgcctg ccagatgacc acgttgtcac
    16141 gcgcgatcac ctcgcccgtc gcggcattgt agctggcgtg gtcgcagcga atttccacgt
    16201 ccgcgtaact gatgctcaca tcccccttcg cgatcgccag gcccgtgtcg gggtcatagg
    16261 tcgtctccat gccctcgatg ttcaaattgc tcgtcagcga gtcgaagaac gcctgtcctc
    16321 gcgcgcctgc agccatcagc aggctggcaa ggacgagcgc ggcgcggagt cgggaggaaa
    16381 tcatgatgcc gggaggaatg aaaatgaaat gtccgggccg ggttcgccag ccgggtcaaa
    16441 acgcgcattc cataggccag ggcctccaaa gtgacaagaa aaacgaaacg gaaatccagc
    16501 ccccgcctca gtcctccccg ccgcgccacg cgcagacgcc ccgcctcccc gggtgctcca
    16561 caaactccag catccgtagc gacgggccgc tccactcccg cccgcgcgcc agggagacag
    16621 cctgggtgat attcctgccc gagcaaaaga gcagccggaa catctccttg atttccgcgc
    16681 gctccgccgc agtgaagccc tgccggcgca ggcccacgac attcagcccg gtgacgcggt
    16741 tcgtgcgcat cgccgcgcaa aaatgcggga tgtccttgcc gaagctgccg ttcccctgca
    16801 ccacgcagga gtcgccgatg cggatgaact ggtgaaaaac cgccccgccg ccgatgaagg
    16861 tgcggctgcc cacccgcaca tggcccgcca gcaggcaggc attggccagg atgttgccat
    16921 cccccagctc cacatcgtgc ccgaggtgcg cgcccaccat gatgaaattc ccgtcgccga
    16981 cacgggtgac cgaccccggc ttgctgccgc ggtggatcgt cacatgctcg cgaatgatgt
    17041 ttttttcacc gatgaccaca gagctttccg tcgccggatc ataacccgtg tcctgcggct
    17101 ccccgccgat caccgccccc cggcccacca ccgtgcccgc gcccagcacg accctgcccg
    17161 ccagttgcgc gtgcgcctcg atgcggcagc cggggccgat ctccgccggg ccgtcaatca
    17221 ccgcgtacgg ccccacttcc acatcttcgg cgaggcgggc ggcggcatga acaatggcgg
    17281 tcgggtggat cattggcggc gagtttatcg gcgggaaaag cgcgccttca actcaaactc
    17341 cggcgccaca cgcgcgcgcc cgctcaactg caaatctcca ccgcctgacg cagggaggcc
    17401 agcttgtccg gctgtttcgg gatgaactcc tggccgagga aacccttgta accggtctcc
    17461 cggatggctt tgacgatcgc cgggtagtgc agctcctgcg tgccgtctat ctccgcgcgt
    17521 cccggcacgc cgcctgtgtg gtagtggctg aaaaaggcgt gccgggcgcg gatggtggcg
    17581 atgacatccc cttccatgat ctgcatgtgg tagatgtcat acagcagttt gaaatgctcc
    17641 gagccgagct tttcacacaa ggccgcgccc caggcgctgc ggtcgcacat gtagtccttg
    17701 tggttcacct tgctgttgag cagctccatt gtcagcgtca cgccaagctt ctccgccagg
    17761 ggcagcaggc ggcgcaggcc cgccgcgcag ttttccagcc cctgctcgtc gcccatgccg
    17821 tcgcggttgc cgctgaagca gatgatgttt ttgaagcccg cctccgcgct ggtcttcatc
    17881 agcggctcgt aggcctggac gagcaggtcg tggtgctcca cgcggttgaa accgtgcggg
    17941 atgctgccga tttttttgcc atccggcccc tgcgccgtgg ggaaactgac cattgcgcaa
    18001 tgcaggccgt gctttttcaa caccgggaaa tccggcggat ccagcagctc cacggactcg
    18061 atgccaatct ccttcgccgc cgcgcacatc gcctccaggg ggatgtcctt gtaacaccac
    18121 ttgcagactg aatggcgcag cccgcccgcg ccgccggcgg cccaggcctg ctcccggagc
    18181 agag
//
LOCUS       DAOMYJ010000087        17986 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Prosthecobacter sp. isolate AMR_MDS_5629
            NODE_1075_length_17986_cov_22.135018, whole genome shotgun
            sequence.
ACCESSION   DAOMYJ010000087 DAOMYJ010000000
VERSION     DAOMYJ010000087.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867850
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prosthecobacter sp. (wastewater metagenome)
  ORGANISM  Prosthecobacter sp.
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Verrucomicrobiaceae; Prosthecobacter.
REFERENCE   1  (bases 1 to 17986)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17986)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.30
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:34:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,742
            CDSs (total)                      :: 4,684
            Genes (coding)                    :: 4,676
            CDSs (with protein)               :: 4,676
            Genes (RNA)                       :: 58
            tRNAs                             :: 55
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17986
                     /organism="Prosthecobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1075_length_17986_cov_22.135018"
                     /isolate="AMR_MDS_5629"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1965333"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1640)
                     /locus_tag="PLP58_10785"
     CDS             complement(<1..1640)
                     /locus_tag="PLP58_10785"
                     /inference="COORDINATES: protein motif:HMM:TIGR03663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03663 family protein"
                     /protein_id="HRJ09047.1"
                     /translation="MNPRKETRLSITTFLVLVLGALVLGGFYRLPHLDRRPMHTDEAV
                     LGMKLADYWQSGHFDYDPKDYHGPGLHQVSKLWGMAAGWGDSATWTESDLRLVAALCG
                     MALLLLTLLFADALGRAGTALAMTLTAVSPMMVFYSRYFIMEMLLAMLVALSLGSFWR
                     FSQGGTRLWLVLGGCALGMQHATKETFILNAGAACCGWVAARVLVGGFSPRQNGGLQL
                     GPAKSSTRPASPWLWTAVPAVFVSVAAFSSWFQDWTAVRESVTCYLDYLERGKGSGHQ
                     KPWHYYLTLIFWRKDTLVWSEAMIGGLALAGMLHAFFGDHQRKAARQAFLVFLTVYTL
                     ALLTVYSLISYKTPWSILCAQHALILLAGAGGGAIWAALPNVMLRGLFHLLVASGLYH
                     LCSQTMRLTGTHANPQLEYSADPRNPYVYSHPPKSLFKLLDLVRSHIGGKTDTARVQV
                     IQKDSGWPLPWYWRAWPLTGWHTAPPDSLDADVIVVEADYYDAVKEKIRAEDYIERSP
                     FGLRPGVIVSVFLRKTPLAESASGTPAPAPPAPGEPLTP"
     gene            complement(1668..2498)
                     /locus_tag="PLP58_10790"
     CDS             complement(1668..2498)
                     /locus_tag="PLP58_10790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009961698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HRJ09048.1"
                     /translation="MRRLCIHTITTKPWSTEECIRNYSRAGAGGITFWRYNLEGRDPA
                     AVGRQAREAGLEVTSLCRGGFFPASTAQDRQKALDDNRRCIEEAHALGAPLIVLVCGA
                     VPGQPLDESRKQITEGIAAVLPEAEAAGVRLGIEPLHPMYADDRSAVNTMRQANDICD
                     ALDSPQMLGIAADVYHIWWDPELKEQIGLTAELGRLFAFHICDWRTPTTDLLNDRSLM
                     GEGCIPIRQISEWVDAAGFQGAREVEIFSSRWWSQDQHEWLEKITEAYRALYEIPAGA
                     "
     gene            2650..6483
                     /locus_tag="PLP58_10795"
     CDS             2650..6483
                     /locus_tag="PLP58_10795"
                     /inference="COORDINATES: protein motif:HMM:NF016260.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translocation/assembly module TamB
                     domain-containing protein"
                     /protein_id="HRJ09049.1"
                     /translation="MSESSASKSKPRRFWKWLRRFLVAGFLLLMLLAVFHKPLLLTVI
                     RVAGPKLAAGQGLELDWQVGGTLWDAFALQKVEVIGRREHWLPAAKLEELRLGYDLRA
                     LWRGEHTRVVRQVRVHGLKATADLRRLPAKPAEVTEAPKEAGSQKPTALWPQELDIQD
                     ADLDLTLADGARLLVEGLTLQAGGGQPGIFECRALRREPGGPALENLRAKIEWETLRL
                     TVADFQLPEDFLLERLVLDLAGLWCEEDEALAELRLRLGDATLELDAKVAGVLEPPMR
                     VSAEVRLDGLRSDDVKAWGLPEHAGFAKASVQARVEGDPAAPGSMSVDVSVELEGVAA
                     AGARVDDVKIHATVKDGKAALHEARVARGENVITITAGAVLPADPGDLPKTPWTAGLK
                     ASLPDARTFLDAAPPVQGSLTLTVNAAGKGPTPTSVEGVLTGTALAFEKWRLPELKAV
                     FGMDGMEARVELPDLALGQGNVIALKSSMRMEDAMPVRAEWAVKIADAALALRTAGME
                     LPAQEFGAVVESEGNAGFAVRDVEAGDFRALTAEASLRVREARHGQARLEEAALEMKA
                     REGRVELETMKLRMDEENHLDLNGGMDLQAPHAFEAKGDVTLPALVRFNELLKSLGAP
                     ELKSGSVEAALDVKGQLQPWTCEGRAGLQARRLQTAAMPHAADASLEATFAGKRADLS
                     KLEAGAGPWRLLARGVVDEKRADLAELKVWQKEALLLDGHARAPLDLMDTQEGEGGPL
                     DVRLRAEKLRLHEVLAEAGIQNVPPGVLDAELALQGRLDTLMADIQARLADLKVPGAP
                     KAFQPASVDFSGKLEAGRLGAKVIVAQPPLKDLTVEAALPVDVPALMKQPDALMKTPL
                     QASASLPESGLDFLREYAPGMVRALPAKLRLDARVTGTAEKPLIEAALDVDAPEIIWA
                     KPDLPSVRDVRARIRAKDRLVMIEDISAVLAGGTVRIGGRVDLQDAQKPAFDARLDAR
                     EALIYRDPGASLRADAAITCRGDLAAARVEGLVELVRGRIFKEIDLLPVLKLPADVPA
                     VPPDTSRQEARLELPPALHGWTFDVGVRTRDPLLISGNLANGAVSADVRLGGTGAAPL
                     LTGGANVDRLLLKLPFSMVKITKGAVTLRPERPFDPDLDIRGESRVGSNDITLFVYGH
                     STDPRTRFTSSPPMSEPDIVTLLATGTTLGGSAAELASEAASRAAVLFLTEMYRKMFN
                     KKKVVREEPPRLHLTFNPSGADRGSDSVQATYDLTENWRLTNRFTQAGRMKTLLGYVL
                     RFGKAARAVDEVKAP"
     gene            complement(6504..7817)
                     /locus_tag="PLP58_10800"
     CDS             complement(6504..7817)
                     /locus_tag="PLP58_10800"
                     /inference="COORDINATES: protein motif:HMM:NF013428.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HRJ09050.1"
                     /translation="MPYPASIPASVDLSKLGEPVYHGSVQNLHAVPDHPGYVVCETTP
                     AGSVFDVGSIFEIPGNDLNRAIFRHAIYLRLGRPETWQKVDEALLGADSLGDTWRNQL
                     RDGPLQAMLERGARTHHLGMVDAVSGGIATGGMPANPSRFNVVKRFPVIRPPQRNVMG
                     SFVFDYAQFHQSDMYVVPLEYIVRFGVTSGSSVLRKYEQLSDKERRAYERELGLSGPM
                     RAWQMLDRPVYDLTSKYEPEDRAVTKQEALLMSGLSALHFTETIKMSLLGAWAVRELL
                     SEIGLHLWDLKWEFAVDREELFFVDTLDADSFRATSTVEVDGRALIIHHNKQAMRDYY
                     RILCADWHEGVNAAKQDARQSGAPFKQVLRDGQESGKYPATPDVDTAFLEIQARKSAL
                     IKAHVLGAMDAAAIAGGLDEAGLAEAEYYRAHGKLGELLGINALP"
     gene            7947..8471
                     /locus_tag="PLP58_10805"
     CDS             7947..8471
                     /locus_tag="PLP58_10805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ09051.1"
                     /translation="MREVFDLLLRIQKRRDVSLLLIGGWAVQAHGFTRATLDVDCLSA
                     VEDEAKIAEELEKAGFVCFDEKPSFRRFQHKIDPLMILDVMRVNAATFEKMRALSESY
                     TVSGIEFRVPSLAHLIALKLHAARNEHRAQKDLTDVRELLRANPGKVAPDELRQLCRQ
                     FGTPQLAAQLEDHL"
     gene            8468..8695
                     /locus_tag="PLP58_10810"
     CDS             8468..8695
                     /locus_tag="PLP58_10810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ09052.1"
                     /translation="MNPGLDLELPFDDMPPREPVRMDADNYLAFIEFNQGILRENGLV
                     QRVLSLRAEPVAHLFTLAPARLECANQEASL"
     gene            complement(8735..10054)
                     /locus_tag="PLP58_10815"
     CDS             complement(8735..10054)
                     /locus_tag="PLP58_10815"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HRJ09053.1"
                     /translation="MFLSAAALHAAPNPVWDNPLFQKKLLGSFGFSSEVEPPMSETEM
                     ADYEQILPLIKDDPDTALQYLEKLRALPDSSARFDFLIGNLHFQNDRKQQAAADFIKA
                     IGKFPDYRQAHANLAILYAQAGAHKEAVRHFTEAIRLGAVDATNYGLLAISHLTLENF
                     TAAEEAFRLAMVLAPDIKDWEMGLMRSLYMQERFPEVVSMLDRMIAANPADDSLWTLQ
                     ANAHMGRKDMLAAAANYEVLHQMGKLETAQLSTLGDIYVNEGMLEAAADAYLRAYEKT
                     GGGDVSVPLRAAEILLAKEGFKASRGLLQKVQSAGESKLTPEDRRRVMKLEAKIGMAE
                     GREAEAVKILTQVAEQDPLDGETLLMLGGYYQKEGNNEKAAFYYETAGNIEAFEADAK
                     TRLAQLYTGMGKYAEAIPLLKRAQDLKPRDSVAKFLEDLERFMKSRR"
     gene            complement(10244..10909)
                     /locus_tag="PLP58_10820"
     CDS             complement(10244..10909)
                     /locus_tag="PLP58_10820"
                     /inference="COORDINATES: protein motif:HMM:TIGR01352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0031992 - energy transducer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HRJ09054.1"
                     /translation="MPQPHPEATTGKRKGAGLWVLVQLLFMLGYGLVLTVFVFGIIPY
                     LQIVTGGGKKDMLVRSVAAISAPPPPDSMLDEPPPPPDAEEEPPPDMQPPPDLSPASL
                     DIGGPVGGGGAAMAGNFGAAAMQNAMANFSMGDMGQKPRPVYQVQPKIPANFRNAAGR
                     IEVEFVVDTQGRVQSPRVTSSFNAALNEPVLAAIKQWRFEPGQRGGRRVPYKTKMPFK
                     FGK"
     gene            complement(10917..11324)
                     /locus_tag="PLP58_10825"
     CDS             complement(10917..11324)
                     /locus_tag="PLP58_10825"
                     /inference="COORDINATES: protein motif:HMM:NF014525.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HRJ09055.1"
                     /translation="MARKFGSSNDGEDDAAIDISPLIDCIFILLIFFIVTTTFVEETG
                     IEVDKPQAASASDLEKQSVMIAITSEGQIVYGGKEIGMTGVQPTVRRFVEKEESTPVI
                     IQVDEKAPSGLFVRVIDEAKLGGALKVSVSTRK"
     gene            complement(11357..12046)
                     /locus_tag="PLP58_10830"
     CDS             complement(11357..12046)
                     /locus_tag="PLP58_10830"
                     /inference="COORDINATES: protein motif:HMM:NF013760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HRJ09056.1"
                     /translation="MTFPLLASLTDSLPEGARAYLQSALELWDAGGWGMIPLSLTAFV
                     LYAKCGDMFYTFFLIHYRQRKYFGGRQLISRLGADSAASISAWRSAQRYLDFHEVRLR
                     DEAGYDEVMAAFEELRAHELPPLDRDLKFITVAIAAAPLWGLLGTVTGMLTTFDGLSK
                     GGGGEGTMNVVAGGISEALITTETGLMIALPGYFLHYLLTSRRARFEAFIEHLQNACG
                     QRALKRHKQHA"
     gene            complement(12077..13552)
                     /locus_tag="PLP58_10835"
     CDS             complement(12077..13552)
                     /locus_tag="PLP58_10835"
                     /inference="COORDINATES: protein motif:HMM:NF013760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015252 - proton channel activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HRJ09057.1"
                     /translation="MRHSLCLSLSLALALSAAAQTTNPLPKVAEDFQKKLDAVTRQLA
                     TTRSQIEEEKVPMTRQLNELDDKVIEARKEYDRVLRIADTRQLDVTNLRAQIKAKEDQ
                     TNYIANAVDEFIRNMETRLHISEAQRYGDTITARKNAANNANLSAEERLQARMDLLML
                     AVDRLEDVLGGTIFDGSAVEGKSGIVSEGKFLVMGPLAFFAGNDGRHMGLADLRLGSL
                     EPAILTLPPETRPEDIRTTLAAGEGSLPVDSTRGSAFKIEETKMTVWQEFLKGGPVMW
                     PIGILGAVALLVGLVKWLQLWLVRRPGHRQISALLEHLDAGRFQEAGAIVRRTGGPVG
                     RMLDAVLKHYRDPVSMMEESMFERVLDAKTRLNAYIPFIKIAAAVEPLLGLLGTVTGM
                     INTFKLITVFGTQDASTFSSGISEALLTTEWGLITAIPCLLMAAFLARMSKAIQDDME
                     KLGVRILNHRNAKDILGAAPRASEPPAKDDPAPGALPAPAV"
     gene            complement(13579..14385)
                     /locus_tag="PLP58_10840"
     CDS             complement(13579..14385)
                     /locus_tag="PLP58_10840"
                     /inference="COORDINATES: protein motif:HMM:NF023358.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3450 family protein"
                     /protein_id="HRJ09058.1"
                     /translation="MFLSRLTAAFRLLLPVFALAASVQAQTPASATKDYLALSRELLN
                     KYYETQKLLAREEADWRAGRDILTSRMALLEAQLKELAEKTAEQTKTISTNDTEREKL
                     DAQNQELKASQDLQLASIEKLETRVHALWPRLPLYLKDKLQGQYDRLPKAEMKKEDIK
                     ASTGERFVNVLVILNEMNKFNSDVLVINERRKVKDGREIEVRVIYFGLAAAYFAGSGE
                     TGDVGGILIPGKDGWEAMEDSTMAPLINEVIAMNKGEKVAGFVSLPVKVR"
     gene            complement(14462..15520)
                     /locus_tag="PLP58_10845"
     CDS             complement(14462..15520)
                     /locus_tag="PLP58_10845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ09059.1"
                     /translation="MKSRQILRAAFALILCTGWTPQSFVPRLNDAGRAGNGTTLLPVR
                     GEPLPPGKNALWCATLQMAWDKACGDFGGPIRLLPASPAAEALNRGRFNPAWIDNESV
                     LTFGGLAGDGVLDAIEKGIKTRGWPASRMVPALRKDILPGDLVFHAVLSKSLSFPEPF
                     AKLGHTRVGRKSTSWFGFHPQLLEANRLREQVRVHDYQSDENFVIELASKNSLDQLIL
                     ARIDTPPRSLDAVSKQLISRLKRAAPHALPADVLAVPNIVVDEKNSFPQIEGRKVAGS
                     GIYLRRALQTVDFRMDEKGVKLRSEASIAFGCSAHYSTGQPRLFILTPPFALLMKRTG
                     APEPYFIAWIANLDALAD"
     gene            15619..15939
                     /locus_tag="PLP58_10850"
     CDS             15619..15939
                     /locus_tag="PLP58_10850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ09060.1"
                     /translation="MTNPIHASVRDASHLESESSGVRQKISGAEFFLDAEGAVAFACA
                     GKSSHESTHEQVFAPGLPDGVQPPARAFGSAANVATKSLSGKCDKSWRSCKEGGWEKT
                     IRVM"
     gene            complement(16052..16306)
                     /locus_tag="PLP58_10855"
     CDS             complement(16052..16306)
                     /locus_tag="PLP58_10855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ09061.1"
                     /translation="MKTFGLISQLAQTRVTLPLIHIETRFRVTGEFAAVEMDQVFEQT
                     AREPLDVTPLRVQRPKASCTVERGGYSLGSMRHWQPVFSR"
     gene            complement(16317..16790)
                     /locus_tag="PLP58_10860"
     CDS             complement(16317..16790)
                     /locus_tag="PLP58_10860"
                     /inference="COORDINATES: protein motif:HMM:NF024803.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HRJ09062.1"
                     /translation="MNLSVDQLADRVTDWCARHRVVPANGQAATATTVRTLRYYRTTG
                     VLDAPAEGGGMGYGERHFLQACSVRVLQAEGLPLSRIQSLLFGRSDDELRAILTSAEQ
                     GNAPHLPEPAAAPVQPESWQTYPLGPDFLIARRPGQRITPAQISAIQAILQSSEN"
     gene            16932..17564
                     /locus_tag="PLP58_10865"
     CDS             16932..17564
                     /locus_tag="PLP58_10865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ09063.1"
                     /translation="MTSWTDFCALLKLHAEPVVLLEGRRSITPADAVKAVRMGRFLAE
                     QFPAGRFRSGNAEGSDAAFAEGVAMVDPARLEVVTPYPGHRHKARVLGADYAAPCDAD
                     RLREPELLAQTVQATPGNQRLIAQYGRSGKGGAKAAYLVRDTLKVLGIEGRLVRPVAA
                     LFWVDPSDPEAGGTGHTIRVCRQNDVPVYFQDTWGAWLPDTVRASYRARQ"
     gene            complement(17677..>17986)
                     /locus_tag="PLP58_10870"
     CDS             complement(17677..>17986)
                     /locus_tag="PLP58_10870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ09064.1"
                     /translation="PTLPVTPAVPSPSAAPIRLPAAPDPLEQLRAQYTLTVQDAAQQA
                     LNKLLFDDLPHLQRELTLLQNGSEIPDLDEPTLPSSLKQLRQNYREARARILAPKQAK
                     "
BASE COUNT         3092 a         5331 c         6242 g         3321 t
ORIGIN      
        1 ggggtgagtg gttcgccggg cgcgggcggg gcgggggcgg gtgtcccgga cgcgctttcg
       61 gccagcgggg tcttgcgcag gaagaccgag acgatgacgc ccggacgcag tccgaaggga
      121 gagcgctcga tgtagtcttc ggcgcggatt ttctccttca ccgcgtcgta atagtccgcc
      181 tccaccacga tgacatccgc gtccagggag tccggcggcg cggtgtgcca gccggtcagc
      241 ggccaggcgc gccaatacca gggcagcggc cagcccgagt ccttttgaat gacctgcaca
      301 cgcgcggtgt ccgttttgcc gccgatgtgg gagcgcacca ggtccagcag cttgaagagg
      361 ctcttcggcg ggtgcgaata gacataggga ttgcgcgggt cggcggagta ttcgagttgc
      421 ggattggcat gggtgccggt caggcgcatg gtctgcgagc acagatggta caggccggag
      481 gcgacgagaa ggtggaaaag gccgcgcagc atcacatttg gcagcgcggc ccagatggct
      541 ccgccgcccg ctccggccag caggatcagc gcgtgctgcg cgcataggat ggaccacggc
      601 gtcttgtagc tgatgagcga gtagaccgtg agcagggcca gggtgtaaac ggtgagaaag
      661 accaggaatg cctgccgcgc ggctttcctc tgatggtcgc cgaaaaaggc gtgcagcatc
      721 ccggccagcg ccaggccgcc gatcatcgcc tcgctccaca cgagggtgtc ctttctccag
      781 aagatcagtg tcaggtagta gtgccagggc ttctgatggc cgctgccctt gccgcgctcc
      841 aaataatcca ggtagcaggt cacgctttcg cgcacagcgg tccagtcctg gaaccaggag
      901 gaaaaagccg ccactgagac aaagaccgcc ggcacagccg tccacagcca ggggctggcg
      961 gggcgtgtgc tgcttttggc cgggccaagc tgcaagccgc cgttctggcg cgggctgaac
     1021 ccgcccacca gcacgcgcgc ggccacccag ccgcagcagg cggcgcccgc gttgaggatg
     1081 aaggtctcct tggtggcatg ttgcatgccc agcgcgcagc cgcccaagac cagccacagg
     1141 cgcgtgcctc cctgggagaa gcgccagaag ctgcccagtg acagcgccac aagcatggcc
     1201 agcagcatct ccatgatgaa gtagcggctg taaaagacca tcatcggcga gacagccgtc
     1261 agcgtcatcg ccagcgccgt gccggcgcgg cccagcgcgt ctgcgaacag cagcgtcagc
     1321 agcagcagcg ccatgccgca gagcgcggcc accaggcgca ggtcggactc ggtccatgtg
     1381 gcggaatcac cccagccagc ggccatgccc cagagcttgc tcacctggtg caggccgggg
     1441 ccgtggtagt ccttggggtc gtaatcgaaa tgtccgcttt gccagtagtc cgccagcttc
     1501 atgcccagca ccgcctcgtc cgtgtgcatg gggcggcggt ccagatgcgg cagccggtaa
     1561 aagccgccca gaaccagcgc gccgagcacc agcacgagaa aggtggtgat ggaaaggcgg
     1621 gtttctttgc gcggattcat gggacggcca tcatggagtc tgcgatctca tgcgccagcg
     1681 ggaatctcgt agagggcacg gtaggcttcc gtgatttttt ccagccattc atgctgatcc
     1741 tgtgaccacc agcggctgga gaaaatctcc acctcacgcg cgccctggaa accagcggcg
     1801 tccacccact cggaaatttg ccgaattggt atgcagccct cgcccatcag gctgcggtcg
     1861 ttgagcaggt ctgttgtcgg tgtcctccag tcgcagatgt gaaacgcgaa aagccggccg
     1921 agctccgccg tcagcccgat ctgctctttc aactccgggt cccaccagat gtggtacaca
     1981 tccgccgcga tgcccagcat ctgcggcgag tcgagcgcgt cgcagatgtc gttggcctgg
     2041 cgcatcgtgt tcaccgcgct gcggtcgtca gcatacatcg ggtgcagcgg ctcgatgccc
     2101 aggcgcactc ccgccgcctc cgcctccggc agcacggcgg cgatgccctc cgtgatctgt
     2161 ttgcgcgact catccagcgg ctgccccgga accgcgccgc acaccagcac gatgagcggc
     2221 gcacccagcg cgtgcgcctc ctcgatgcag cgccggttgt catccagagc tttttgcctg
     2281 tcttgcgcag tggacgcagg aaaaaagccg cccctgcaca ggcttgtcac ctccagtccc
     2341 gcctcccgcg cctggcggcc caccgcagcc gggtcgcgcc cctccaggtt gtagcgccag
     2401 aaagtgatcc ctccggctcc agcccgcgag tagttgcgga tgcactcttc ggtgctccag
     2461 ggcttggtcg tgatggtgtg gatgcagagc cgtcgcatgg gtgtggggat gtcgttggga
     2521 ttaacccagc ggcaaaaaca aacaatcacc gtctggtcaa gcctgacttg agggcggaaa
     2581 ccgcgcgccc tgtctcttgt ctgtcgcgcg aatccttgcg gcctggatca tttcaggcga
     2641 ctaacgcgcg tgtccgagtc ttccgcatcg aagtccaaac cccgccgttt ttggaaatgg
     2701 ctccgccgat tcctggtcgc gggtttcctt ctgctcatgc tgctggccgt gtttcacaag
     2761 ccattgcttt taaccgtgat tcgcgtggcg ggaccgaagc tggcggcggg ccagggtttg
     2821 gagctcgact ggcaggtcgg cggcactctg tgggatgctt ttgcgcttca aaaggttgag
     2881 gtcattggca ggcgggagca ttggctgcca gcggccaaac ttgaggaatt gcgcctaggc
     2941 tatgatttgc gcgcgctttg gcgcggcgag cacacgcggg tggtgcggca ggtgcgggtg
     3001 catggcctga aagcgacggc ggatttgcgg cggctgccgg cgaagcctgc cgaagtgaca
     3061 gaggcaccca aagaagccgg ttcccaaaaa ccgaccgcgc tttggccgca ggaactggac
     3121 atccaggacg cggacctgga cctgactctg gcagatggag cgcggctgct ggtggagggg
     3181 ctgactttgc aagcaggcgg cgggcagccc ggcatcttcg agtgccgcgc gctgcgccgc
     3241 gagccgggcg gccccgcgct ggaaaatctg cgcgccaaga tcgaatggga gacgctgcgg
     3301 ctgacggtgg cggattttca actgcctgag gattttctgc tagagaggct cgtgctcgat
     3361 ctcgccggtc tctggtgcga ggaggatgaa gcgctggctg aactgcggct gcgccttgga
     3421 gatgccacgc tggagctgga tgccaaagtg gcgggtgtcc tggagccgcc gatgcgggtg
     3481 agtgcggaag tgagactgga cggcctgcgc tcggatgatg tgaaagcgtg gggcctgcct
     3541 gagcatgcgg gctttgccaa agcctccgtg caggcgcggg tggagggcga cccagccgcg
     3601 ccgggttcca tgagcgtgga tgtctcggtt gagttggagg gcgtcgcagc ggcaggcgcg
     3661 cgtgtggatg acgtgaaaat ccacgccact gtcaaggatg gcaaagccgc cctgcatgag
     3721 gcgcgggtgg cgcgcggtga aaatgtcatc acgatcacgg ccggcgcggt tttgcccgct
     3781 gatcccggcg atctgccaaa gacgccgtgg accgccggat taaaggccag cctgccggat
     3841 gcgcggactt ttctcgatgc agcccctccc gtgcaaggct cgctgacgct gacagtcaac
     3901 gctgctggca aaggaccgac gccgacgagc gtcgagggtg tcctcaccgg cacggcgctg
     3961 gcttttgaaa aatggcggct gccggagttg aaggcggtgt tcggcatgga tggcatggag
     4021 gcgcgcgtgg agctgccaga cttggcgtta ggccagggca atgtcatcgc cttgaaatcc
     4081 tcaatgcgca tggaggacgc gatgccggtg cgcgcggagt gggcggtgaa gatcgccgac
     4141 gcggctctcg cgctgcgcac cgccggcatg gagcttccgg cgcaggagtt cggcgctgtg
     4201 gtggagtcgg aaggcaatgc cggattcgcc gtgcgggatg tggaggcggg ggacttccgc
     4261 gctctgaccg ctgaagcaag cctgagagtg cgcgaggcgc ggcacggcca ggcgcgcctg
     4321 gaggaggcgg ctttggagat gaaggcgcgc gaggggcgtg tggagttgga aaccatgaag
     4381 ctgcgaatgg atgaggagaa ccatctcgat ctgaatggcg gcatggacct tcaggcgccg
     4441 cacgccttcg aggcgaaggg ggatgtgacc ctgcccgcgc tggtccgctt caatgaactg
     4501 ctcaagagcc tcggcgcgcc cgaactgaaa agcggctcgg tggaggccgc gctggatgtg
     4561 aagggccaac tgcaaccctg gacctgcgag ggccgtgccg ggctgcaagc gaggcgcctt
     4621 caaacggcgg cgatgcccca tgccgcggat gcgagcctgg aggccacttt cgcaggcaag
     4681 cgcgcggatt tgtcgaagct ggaggccggc gcgggtcctt ggcggctgct ggcgcgcggc
     4741 gtggtggatg aaaaacgcgc cgatctggcc gaactgaaag tgtggcaaaa ggaggcgctg
     4801 cttctcgacg gccatgcgcg cgcgccgctc gatctcatgg acacgcagga gggtgaaggc
     4861 ggcccgctcg atgtgagact gcgcgccgaa aaactccgtc tgcacgaggt gctggcggag
     4921 gccggcattc aaaatgtgcc gccaggcgtg ctggacgcgg aactggctct ccaaggccgc
     4981 ctcgacacgc tgatggcgga catccaggcg cggctggcgg atttgaaagt gcccggcgcg
     5041 cccaaggcat tccagccagc cagcgtggat ttcagcggca aactggaagc gggccgtctc
     5101 ggagcaaagg tgatcgtggc gcagccgccg ttgaaggatc tgaccgtcga ggcggcgctg
     5161 cccgtggatg tgcccgcgct catgaagcag ccggacgcgc tcatgaaaac gccgctgcaa
     5221 gcgagcgcgt ccctgcccga gagcggcctg gatttcctgc gcgaatacgc gcccggcatg
     5281 gtccgcgccc tgcctgcgaa attgaggctc gatgccagag tgacaggaac cgcggagaag
     5341 cccctgatcg aagccgcgct ggatgtggac gcgccggaga tcatctgggc caagcctgac
     5401 ctgccctccg tgcgtgatgt gcgcgcccgc atccgcgcca aagaccggct cgtgatgatc
     5461 gaggacatct ccgccgtgct ggcaggaggc acggtcagga tcggcggcag ggtggatttg
     5521 caggacgcgc aaaagcccgc cttcgatgcg cggctcgacg ccagggaagc tctcatttac
     5581 cgcgaccccg gcgcttccct ccgcgctgac gcggccatca cctgccgggg cgatctggcg
     5641 gcggcgcgtg tcgagggcct ggtcgagctg gtgcgcggcc gcatcttcaa ggagatagac
     5701 cttctgcctg tgctgaaact gcccgccgat gtgcccgccg tgccgcccga caccagccgc
     5761 caggaggcca ggctggagct gccgcccgcg ctgcacggct ggaccttcga tgtgggcgtg
     5821 cgcacgcgcg atccgctcct catctcgggc aatctggcca atggagcggt ctccgccgat
     5881 gtccgcctcg gcggcacggg cgcggcgccg ctgctcacgg gcggggccaa tgtggaccgc
     5941 ctgcttttga agctgccctt cagcatggtg aagatcacca agggcgccgt caccctgcga
     6001 ccggagcggc cctttgatcc cgatctcgac atccgcggcg agtcgcgcgt cggcagcaac
     6061 gacatcacgc tttttgtcta tggccactcc accgacccgc gcacgcgttt caccagctcg
     6121 ccgccgatga gcgagccgga catcgtcacc ctgctcgcca ccggcaccac tctcggaggc
     6181 tccgccgccg aactggcctc cgaggccgcc agccgcgcgg cggtgctgtt cctcaccgaa
     6241 atgtacagga agatgtttaa caaaaagaaa gtcgtgcgcg aggagcctcc gcgcctgcac
     6301 ctgaccttca acccctccgg cgcggaccgc ggcagcgaca gcgtgcaggc cacctacgat
     6361 ctcacggaaa actggcgttt gaccaaccgc tttacccagg ccggccgcat gaagacgctg
     6421 cttggatatg tgctgcgctt cggcaaagcg gcccgggcgg tggatgaggt caaggcacct
     6481 taagggggcg gcgcttcctg gtttcacggc agggcgttga tgcccagcaa ttctccgagc
     6541 tttccatgcg cgcggtagta ttccgcctcg gccaggcccg cttcatccag gccacccgcg
     6601 atggccgcgg cgtccattgc accgagcaca tgggctttga tgagcgccga cttgcgcgcc
     6661 tggatttcga ggaaggcggt gtccacatcg ggagtggcgg ggtatttgcc ggactcctgc
     6721 ccgtcgcgca ggacctgctt gaagggcgcg ccgctctgcc gcgcgtcctg cttggccgcg
     6781 ttgacgccct catgccagtc cgcgcagaga atgcggtagt agtcgcgcat ggcctgcttg
     6841 ttgtggtgga tgatgagcgc gcgcccgtcc acctcgacgg tgctggtggc gcggaagctg
     6901 tccgcatcca gcgtgtccac gaagaacagc tcctcgcggt ccacggcgaa ctcccatttc
     6961 aaatcccata gatgcaggcc gatctccgac agcagctccc tgactgccca cgcgccgagg
     7021 agggacatct tgatggtctc ggtgaaatgc agcgcgctga ggccgctcat gagcagcgcc
     7081 tcctgcttcg tcaccgcgcg gtcctcaggc tcgtatttgc tggtgaggtc ataaaccggc
     7141 cggtccagca tctgccacgc gcgcattggc cccgagaggc ccagctcccg ctcgtaggcg
     7201 cggcgctcct tgtcgcttag ctgctcgtat ttgcgcagca ccgagctgcc actagtgacg
     7261 ccaaagcgca cgatgtactc cagcggcacc acatacatgt cgctctggtg aaactgggcg
     7321 tagtcaaaga caaaactgcc catcacattg cgctggggcg ggcggatcac cggaaagcgc
     7381 ttcaccacgt tgaagcggct cggatttgcc ggcatgcctc cggtggctat cccgccgctc
     7441 accgcgtcca ccatgcccag gtggtgcgtg cgcgcgccgc gctcaagcat cgcctgcagc
     7501 gggccgtccc tgagttggtt gcgccaggtg tcgccgaggg agtccgcgcc cagcaaggct
     7561 tcatcgacct tttgccaggt ctccgggcgt ccgagacgca ggtatatggc gtggcggaaa
     7621 atggcgcggt tgaggtcatt gccggggatt tcaaaaatgg agcccacgtc gaagacggaa
     7681 cccgccggag tcgtctcgca caccacataa ccggggtggt ccggcacggc gtgcaggttc
     7741 tgcacggagc cgtgatacac cggctcgccg agtttcgaga ggtccacaga ggcggggatg
     7801 gaggcgggat aaggcatggc gggaagagtg gcgagagttc ggcggggatg gggggatttt
     7861 caaatcattc caggctggaa acgcctctcg cgcgcaatgc ccgtcagcgc gcttgtttca
     7921 gccggcaata gctgcatggt gaccccatgc gcgaagtctt tgacctgctg ttgcgaatcc
     7981 aaaagcgtcg ggatgtttcc ttgctgctga tcggcggctg ggcagtgcag gctcatggat
     8041 tcacgcgcgc caccctggat gttgactgcc ttagcgccgt ggaggacgag gccaaaatcg
     8101 ccgaggaact cgaaaaggcg ggttttgttt gtttcgacga gaaaccctcc tttcgccgct
     8161 tccagcacaa gattgaccct ctcatgattt tggatgtcat gcgtgtcaat gcggcgacat
     8221 ttgagaagat gcgggcatta tcagaatcct acaccgtttc aggcatcgaa ttccgcgtgc
     8281 catcgcttgc gcatctgatc gctctgaaac tccatgcggc tcgaaacgag caccgcgctc
     8341 aaaaagacct gactgatgtg cgcgagctgc tgcgcgcgaa tcccggcaag gtcgccccgg
     8401 atgagctgcg ccagctttgc cggcagttcg gcacgccgca attggcggcg caactcgaag
     8461 accatttatg aatcctggcc ttgatctcga actgccgttt gatgacatgc cccctcggga
     8521 gcctgtgcgc atggatgcgg acaactatct ggccttcatt gaattcaacc aggggattct
     8581 tcgtgaaaac ggcctggtgc agcgtgtgct gagcctgcgg gccgagccgg ttgctcactt
     8641 gttcacgctc gcccccgccc gccttgagtg tgcgaatcaa gaggcgtccc tgtaaggcca
     8701 ggatgcgctc gcgtgtgctg atggaatcca agcctcacct ccggctcttc atgaagcgct
     8761 ccaggtcttc gaggaatttg gccacgctgt cgcggggctt gaggtcctgg gcgcgcttga
     8821 gcagcgggat ggcctcggcg tatttgccca tgccggtgta gagctgggcg aggcgggtct
     8881 tggcgtcggc ttcgaaggct tcgatgttgc cggcggtctc gtagtaaaag gcggccttct
     8941 cgttgttgcc ctccttttgg taatacccgc cgagcatgag cagcgtctcg ccatccagag
     9001 ggtcctgctc ggcgacttgg gtgaggattt tcacggcttc agcctcgcgg ccctcggcca
     9061 tgccgatctt ggcctccagc ttcatgacgc ggcggcggtc ctccggggtg agcttgcttt
     9121 cgccggcgga ctggaccttt tgcagcaggc cgcgggaggc tttgaagccc tccttggcaa
     9181 gcaggatctc ggcggcgcgc aggggcacgg agacatcgcc tccgccggtc ttttcatagg
     9241 cgcgcaggta ggcgtcggcg gcggcttcga gcatgccttc gttcacatag atgtcgccga
     9301 gggtggagag ctgggcggtc tccagcttgc ccatctggtg cagcacctcg taattggcgg
     9361 cggcggcgag catgtccttg cggcccatgt gggcgttggc ctgcagagtc cagaggctgt
     9421 cgtcggcggg gttggcggcg atcatgcggt ccagcatgga gacgacctcg gggaaacgct
     9481 cctgcatgta gagggagcgc atgagcccca tctcccagtc cttgatgtcg ggtgccagca
     9541 ccatggcgag ccggaaggcc tcctcggcgg cggtgaagtt ctccagcgtg aggtgggaga
     9601 tggcgagcag gccgtaattc gtggcgtcca ccgcgccgag gcggatggcc tcggtgaagt
     9661 ggcgcacggc ctccttgtgc gcgccggcct gggcgtagag gatggcgagg ttggcgtgtg
     9721 cctggcggta gtcggggaat ttgccgatgg cctttatgaa gtcggcggcg gcctgctgct
     9781 tgcggtcatt ctggaagtgc aggttgccga tgaggaagtc gaatcgggcg ctgctgtcag
     9841 gcagggcgcg gagcttttcc aggtattgca gcgcggtgtc cgggtcgtct ttgatgagcg
     9901 ggaggatctg ctcgtagtcg gccatttcgg tctcggacat gggcggctcc acctcgctgc
     9961 tgaagccgaa gctgccgagc agcttcttct ggaagagggg gttgtcccac acggggttcg
    10021 gcgcggcgtg caaggcggcg gcgctgagga acaaggcggg gatgaattgg ccgcaaaaaa
    10081 acgcaaaaga cgcaaaaagg gtgtttcgga aacagggttt aaagctccca gcatgaagaa
    10141 gacacgagag tctgtctgct ccggcttttt gcgcttcttt gagttttttg cggcgatccg
    10201 ttccgggctg ctggctgggg agactggctt tcatgagtgg aggttatttg ccgaatttga
    10261 agggcatctt ggtcttgtaa ggcacgcgcc tgccgccgcg ctggccgggc tcgaagcgcc
    10321 actgcttgat ggcggcgagc acgggctcgt tgagggcggc gttgaagctg ctggtgacgc
    10381 gcgggctctg cacgcggccc tgggtgtcca cgacgaactc gacctcgatg cgcccggcgg
    10441 cgttgcggaa gttggcgggg attttgggct gcacctggta cacggggcgg ggcttttgcc
    10501 ccatgtcacc catgctgaaa ttggccatgg cattctgcat ggcggcggcg ccgaagttcc
    10561 cggccatggc ggcgccgccg ccaccgacgg ggccgccgat gtcgagcgag gcggggctga
    10621 gatccggcgg cggctgcatg tccggcggcg gctcctcctc ggcatccggc gggggcggcg
    10681 gctcgtcgag catggagtcg ggcggcggcg gggcggagat ggcggccacg gagcgcacga
    10741 gcatgtcctt tttgccaccg ccggtgacga tctggagata ggggatgatg ccgaagacga
    10801 agacggtcag cacgaggccg taaccgagca tgaagagcaa ctgcacgagc acccaaaggc
    10861 ccgcgccttt gcgcttcccc gtggtggctt ctggatgcgg ctgcggcatg gcgcggttac
    10921 ttgcgtgtgg agacggagac tttgagcgcg ccgccgagct tggcctcgtc aatgacgcgc
    10981 acgaagaggc cggagggggc tttttcatcc acctggatga tgacgggcgt ggactcctcc
    11041 ttttcgacaa agcggcgcac ggtgggctgc acgccggtca tgccgatctc cttgccgcca
    11101 tagacgatct gcccctcgga ggtgatggcg atcatgacgc tctgcttctc caggtcgctg
    11161 gcggaggcgg cctgcggctt gtccacctcg atgccggtct cctccacgaa ggtggtggtg
    11221 acgatgaaga agatgaggag gatgaaaatg cagtcaatga ggggggagat gtcaatcgcc
    11281 gcgtcgtcct cgccgtcgtt ggagcttccg aattttctgg ccatggtgcg tgtgggtggt
    11341 ggtgcgtgcg gcggtttcag gcgtgctgtt tgtggcgctt gagcgcgcgc tggccgcagg
    11401 cgttttgcag gtgctcgatg aatgcctcga acctggcccg gcggctggtc agcaggtaat
    11461 gcagaaagta acccggcagg gcgatcatga ggcccgtctc ggtggtgatg agcgcctcgg
    11521 agatgccccc ggcgacgacg ttcatggtgc cctcgccgcc gccgcctttg gacaggccgt
    11581 caaaggtggt gagcatgccg gtgacggtgc cgagcagtcc ccacagggga gcggcggcga
    11641 tcgcgacggt gatgaatttc aaatcccggt ccagcggcgg cagctcgtgc gcgcgcagct
    11701 cctcgaaggc ggccatgact tcatcataac cggcctcgtc acgcaggcgc acctcgtgga
    11761 agtccaggta gcgctgggcg ctgcgccagg cgctgatgga ggcggcgctg tcggctccga
    11821 gccggctgat gagctgccgg ccgccgaagt atttgcgctg gcggtagtgg atgagaaaga
    11881 aggtgtaaaa catgtcgccg cacttcgcat acagcacgaa ggcggtgagg ctgagcggga
    11941 tcatgcccca gccgccggcg tcccagagtt cgagcgcgct ctgcaagtag gcgcgcgcgc
    12001 cctcgggcag ggagtcggtc agggaggcga gcagggggaa agtcatcgtc ttgccggggg
    12061 tgccgggcct ggggcgtcac acagcgggag cgggcagcgc gccgggggcg gggtcgtctt
    12121 tggccggtgg ctcggaggcg cgcggtgcgg cgccgaggat gtctttggcg ttgcggtggt
    12181 tcaggatgcg cacgccgagc ttctccatgt cgtcctggat ggccttcgac atgcgcgcga
    12241 gaaaggcggc catgagcagg cacgggatgg cggtgatgag accccactcg gtggtgagca
    12301 gcgcctcgga gatgccggag gagaaggtgg aggcgtcctg cgtgccgaag acggtgatga
    12361 gcttgaaggt gttgatcatc ccggtcacgg tgccgagcag gccgagcaac ggctccacgg
    12421 cggcggcgat cttgatgaag gggatgtagg cgttcagccg cgtcttggcg tccagcacgc
    12481 gctcgaacat ggactcctcc atcatggaca ccgggtcgcg gtagtgcttg agcacggcgt
    12541 cgagcatcct gcccacaggc ccgccggtcc tgcggacaat cgcgcccgcc tcttggaagc
    12601 gcccggcatc cagatgctcc agcaaagcgg agatctgccg gtggccgggg cggcgcacga
    12661 gccagagctg gagccatttg acgaggccga ccagcagagc cacggcaccg agaatgccga
    12721 tcggccacat gacgggaccg cctttgagaa actcctgcca cactgtcatc ttcgtctcct
    12781 cgatcttgaa ggcggagccg cgtgtggagt ccaccggcag gctgccctct ccggcggcca
    12841 gcgtggtgcg gatgtcctcg ggccgtgtct cgggcggcag cgtcaggatc gccggctcca
    12901 ggctgccgag gcgcaggtcg gccaggccca tgtgcctgcc gtcattgccg gcgaaaaagg
    12961 ccagcggccc catgaccagg aacttgccct cggagacgat gccgcttttg ccctccacgg
    13021 cgctgccgtc aaagatggtg ccgccgagca catcctccag gcggtccacc gcgagcatga
    13081 gcaggtccat gcgcgcctgc agccgctcct cggcgctgag gttggcgttg ttggcggcgt
    13141 ttttccttgc cgtgatggtg tccccgtaac gctgcgcctc ggagatgtgc aggcgcgtct
    13201 ccatgttgcg gatgaactcg tccaccgcgt tggcgatgta gttggtctgg tcctccttgg
    13261 ccttgatctg cgcgcgcagg ttcgtcacgt cgagctggcg cgtgtcggcg atgcgcagca
    13321 cgcggtcgta ttccttgcgt gcctcgatga ccttgtcgtc cagctcgttg agctgcctgg
    13381 tcatcggcac cttttcctcc tcgatctggg agcgggtggt ggcgagctgg cgcgtgacgg
    13441 cgtcgagctt cttctggaaa tcctccgcca ccttgggcag gggattcgtg gtctgcgcgg
    13501 cggcggaaag cgcgagcgcg agggaaagcg agaggcagag gctgtgtctc atcgggatca
    13561 aagaaaaagg agtgtgtttc agcgcacctt cacgggcagg gagacgaagc cggccacctt
    13621 ctcacccttg ttcatggcta tgacttcatt gatgagcggg gccattgtcg agtcctccat
    13681 ggcctcccac ccgtccttgc cggggatgag gatgccgccc acgtcgcccg tctcgccgct
    13741 gccggcgaag taggcggcgg ccaggccgaa gtaaatgacg cgcacctcga tctcgcgccc
    13801 gtccttgacc ttgcggcgct cgttgatgac gagcacgtcg ctgttgaact tgttcatctc
    13861 gttgagaatg acgagcacgt tgacaaagcg ctcgccggtg gaggctttga tgtcctcctt
    13921 cttcatctcg gccttcggca ggcggtcata ctggccttga agcttgtcct tcaaatacag
    13981 cggcagccgg ggccagagcg cgtggacgcg ggtttccagc ttctcgatgc tggcgagctg
    14041 gaggtcctgg ctggctttca gctcctggtt ctgcgcgtcg agcttttccc gctcggtgtc
    14101 gttggtggag atggtcttgg tttgctcggc ggtcttttcc gccagctcct tgagctgcgc
    14161 ctccagcagg gccatgcggc tggtgaggat gtccctcccc gcgcgccagt cggcctcctc
    14221 cctggcgagc agtttctggg tctcgtaata cttgttcagc agctccctgg acagcgccag
    14281 gtagtccttc gtggcgctgg cgggagtctg cgcctggacg gaggcggcca gggcaaaaac
    14341 gggcaaaagc aggcgaaaag cggctgtgag tcgggataaa aacacgcctg acaggtgccg
    14401 gaaaagccct gaatccaaag gcgaataacg tgacgaaaaa gacacagtgg cgtttccggc
    14461 gtcaatccgc cagcgcatcc agattcgcaa tccaggcgat gaaatacggc tccggcgcgc
    14521 cggtccgttt catgagcagg gcgaagggcg gggtgaggat aaacagcctg ggttgacctg
    14581 tggaatagtg agccgagcaa ccgaaggcga tggaagcctc gctgcggagt ttgacgcctt
    14641 tttcatccat gcggaaatcc accgtttgca gggcgcggcg caggtatatg ccgctgccgg
    14701 caaccttcct gccttcgatc tgcgggaacg agtttttttc atccacgacg atgttgggca
    14761 cggcaagcac atcggcgggc agggcgtgcg gcgcggcgcg tttcaggcgg gagatgagtt
    14821 gtttggatac ggcatccaaa ctgcgcggcg gagtgtcgat cctggccagg atgagctggt
    14881 caaggctgtt cttgctcgcc agctcgatga caaagttttc gtcggattgg tagtcatgca
    14941 cgcgaacttg ctcgcgcagc cggttcgcct ccaacagttg cgggtggaag ccgaaccatg
    15001 aggtgctttt tcgcccgacc ctggtgtgtc cgagtttggc aaagggttcc gggaaagaca
    15061 ggctcttgct cagaacagca tggaagacga ggtctccagg caggatgtct ttgcgcaacg
    15121 cgggaaccat tcttgaggcg ggccatccgc gcgtcttgat gcccttttcg attgcatcaa
    15181 gcactccatc tcctgcaagg ccgccaaatg tcagcaccga ttcgttgtca atccatgcgg
    15241 gattgaagcg tccacggttc agagcctcgg ccgccgggct tgccggcagc agacgaatgg
    15301 gaccaccgaa gtctccgcaa gccttgtccc atgccatttg cagagtggcg caccagagcg
    15361 cgttttttcc ggggggcagc ggttctccgc gcacgggcag caaggtcgtt ccattcccgg
    15421 cccggccggc gtcattgagc cggggcacaa aactctgcgg agtccatccg gtgcaaagaa
    15481 tgagcgcgaa cgcggctcgg aggatttgcc ggcttttcat gcgggtcgtg gatggttcat
    15541 gctgttcgtg gcttgagatt tcagcctgtt ggaaatgccg gtcatgcgga atgctattgg
    15601 cggcagtctt ggagttccat gactaatccc atacatgctt cggttcgaga tgccagccat
    15661 ctggaatccg agtcatctgg agtccggcag aagatttcag gtgcggaatt ttttctcgac
    15721 gcggaagggg cggttgcctt tgcctgcgcg ggaaagtcca gtcatgaatc aacccatgaa
    15781 caggtgtttg cccccgggct gccagacggc gttcagccgc cagcccgggc ttttggctca
    15841 gcagccaatg tagccactaa gagcctgtcc ggcaaatgtg acaaaagctg gagaagctgt
    15901 aaagaaggcg gatgggaaaa aactatccga gtgatgtgag cgacgaagag tgggaactcg
    15961 tcaaagcgga gctgtgcgcc gagggcaaac cgcgaggcag ggaacgtgtc gccagcgagg
    16021 catccgcgcg agcctgcttc aatgccctgc gctacctgct gaaaaccggc tgccagtggc
    16081 gcatgctgcc caaggaatac ccgccacgct cgacggtgca tgacgccttg ggccgctgga
    16141 cgcgcaacgg agtgacatcg agcggctcgc gggcggtctg ctcaaagacc tggtccatct
    16201 ccacggcggc gaactcgccc gtgacgcgga agcgggtttc gatgtggatg agcgggaggg
    16261 tgacgcgggt ttgcgcgagt tgggagatga ggccgaaggt tttcatgggg atgcggttag
    16321 ttttcggatg attggaggat ggcttggatg gcggaaatct gggcaggtgt gatgcgctgg
    16381 ccggggcggc gggcgatgag gaagtcgggg ccgagcgggt aagtctgcca gctctctggc
    16441 tgaacgggcg cggcggcggg ttcaggaaga tgcggagcgt tgccctgctc cgcactggtg
    16501 agaatggcgc gcagttcgtc atcgctgcgg ccaaacagga gtgactggat gcggctgagt
    16561 ggcaatcctt ccgcttgaag caccctcacg gagcacgcct gcagaaaatg ccgctcgcca
    16621 tagcccatgc cgccaccctc ggcgggcgca tcaaggactc ccgtcgtccg gtagtagcgc
    16681 agggtgcgga ccgtcgtggc agtggcggcc tggccgttgg cgggcacgac gcgatgtcgc
    16741 gcgcaccagt cagtgacgcg gtcggcgagc tggtccacgg agaggttcat agaatgagtt
    16801 atgttagtga cagtgttact gtcaattcaa acttgcgcat gcttgatcaa gcctcactgt
    16861 ggaagcgatt tccaaccgct gattctgagt ttcgaaaaag ctctgccctg tgatatacct
    16921 gcctcctcct catgacgtcc tggactgact tttgcgcgct tctcaaactc cacgctgaac
    16981 ccgtggtcct gcttgaggga cggcgctcaa tcacgcctgc cgatgcagtc aaagcagtgc
    17041 gcatgggcag attcctggcc gagcagtttc cagcggggcg ttttcgttcg ggcaacgcgg
    17101 agggaagcga cgcggctttc gccgagggcg tggcaatggt ggacccggcc aggcttgagg
    17161 tggtgacgcc ttacccgggg caccggcaca aagcgcgtgt gctgggagcg gattatgcgg
    17221 cgccatgcga cgccgaccgg ctgcgcgaac cggaactgct ggcgcaaacc gtgcaggcca
    17281 caccaggaaa ccagcgcctc atcgcgcagt acggcaggtc cggtaaaggc ggggccaagg
    17341 cggcttatct ggtgagggac accctcaaag tgctgggaat cgaggggcgg ctggtcagac
    17401 cggtggcggc actgttttgg gtggacccgt ctgacccgga ggccggagga accggacaca
    17461 ccatccgggt ctgcaggcaa aatgatgttc cggtgtattt ccaggacacc tggggggcgt
    17521 ggcttccgga cacggtgagg gcgagttatc gggcgaggca gtgatcactc gaagatgcga
    17581 gaagtccgga gtggcaagct ggctgtcgaa tgcgcgcctc aagcaatggg agcctgggca
    17641 atggcactgc cgggatgaag aacatccagc gcggtttcac ttcgcttgtt ttggagcgag
    17701 gatgcgggcg cgggcttcgc ggtagttttg gcggagctgc ttcagggaag aaggcagggt
    17761 gggctcgtcg aggtcgggga tttcggagcc gttttgcaga agggtgagct cgcgctggag
    17821 gtggggcagg tcgtcgaaga gaagcttgtt cagagcctgc tgggcggcgt cctgcacggt
    17881 gagggtgtat tgggcgcgga gctgctccag gggatccggc gcggcgggca ggcggatggg
    17941 ggccgccgag ggcgaaggca cggcgggcgt gaccggcagg gtggga
//
LOCUS       DAOMYJ010000088        17828 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Prosthecobacter sp. isolate AMR_MDS_5629
            NODE_1091_length_17828_cov_24.297755, whole genome shotgun
            sequence.
ACCESSION   DAOMYJ010000088 DAOMYJ010000000
VERSION     DAOMYJ010000088.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867850
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prosthecobacter sp. (wastewater metagenome)
  ORGANISM  Prosthecobacter sp.
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Verrucomicrobiaceae; Prosthecobacter.
REFERENCE   1  (bases 1 to 17828)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17828)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.30
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:34:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,742
            CDSs (total)                      :: 4,684
            Genes (coding)                    :: 4,676
            CDSs (with protein)               :: 4,676
            Genes (RNA)                       :: 58
            tRNAs                             :: 55
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17828
                     /organism="Prosthecobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1091_length_17828_cov_24.297755"
                     /isolate="AMR_MDS_5629"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1965333"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..339)
                     /locus_tag="PLP58_10875"
     CDS             complement(<1..339)
                     /locus_tag="PLP58_10875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ09065.1"
                     /translation="MLEKQRQTGGTPVPHAVTVAYSGVHQAFQLALAAQEEELLRDFL
                     CSLHDAPGKWGGCLAGMLGRARLASRSLPGLDAALTVEHPWPALWKVLRDKLVPARAA
                     DWLAVNDAFDR"
     gene            complement(452..1693)
                     /locus_tag="PLP58_10880"
     CDS             complement(452..1693)
                     /locus_tag="PLP58_10880"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HRJ09066.1"
                     /translation="MGDAHAPQPPRVASTPADHCPEAVPTPPSIAVKVLYALPHPVQY
                     QSPMIRRLVSGGVELSVVYARGEQRFFDPEFNRELAWDRPLMEGYNSLVLGPSEDRST
                     LARLLADHARECGAAAVWAHGWSQPMDLAAWNASARLRLPLMIRGDSTLPGRRGGGLH
                     QRVHRWLYARRFRRVAACLAVGSWNAAFYRAYGVPDERIFQVPYAVDNAFFQRQAREA
                     SSSREILRRSLGLEPGLPVVLFCGKLIAKKDAATLIQALSLAGPSAEARLLVVGDGEL
                     RLVLEKLAEDLIPGRARFAGFKNQSELPAIYELCDVFVIPSTYEPFGLVVNEVMNAGK
                     PVIASDRVGCWPDLVRPGVNGAVFPAGDARALAEVLRPFLLDASLRESAGRASLEIIN
                     RWSFEEDLAGIQAALEFVRGR"
     gene            complement(1597..2592)
                     /locus_tag="PLP58_10885"
     CDS             complement(1597..2592)
                     /locus_tag="PLP58_10885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ09067.1"
                     /translation="MKRELVCYHEGWFAEPAWLQRLLGWQDATLQTDAGQTRVVENSI
                     IVCPDLGSLRPELLKRLGQASGVVLFHVSDEWFRSPLEVYRHFSHVIRSYHHTALELP
                     GITQIPLGPCRDVRMGGKPRLSSERQFVWSFAGQVTATRSSMMECLRDMQPNSLRVTA
                     GAPAAQTMLEPGAYRELLRESVFVPCPMGNVNLDSFRLYEALDAGALPIVERRPWLDY
                     FTRLLGNHPLPSVTSWRHAAVLMRSFVRDKEALDRKQAEIQDWWERVQCRLGFKLAEV
                     LQESCDSVSRPLAAAPLPGMMRSRWEMLMHHNRHALLARLQITVRRLFQRRPRSL"
     gene            complement(2593..3261)
                     /locus_tag="PLP58_10890"
     CDS             complement(2593..3261)
                     /locus_tag="PLP58_10890"
                     /inference="COORDINATES: protein motif:HMM:NF016908.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FkbM family methyltransferase"
                     /protein_id="HRJ09068.1"
                     /translation="MRLSPSDSFHGEIAFCGIYEADESRAIHMLAKEQGGLLVDVGAN
                     YGYYTLLWCAARPDNRAVAIEASPRNAQALRENVARNNLTSRVRVVECAVTRETGRIS
                     FDLGSAEETGWGGIASGLEGGTISVPAERLDRLVSEPVRVLKVDCEGADAWVVEGATA
                     LLDSGNISHVFFEENVWRQERLGIAAGSAQDRLRRAGFEIQGIGKGPPFANHHASHVS
                     SGGC"
     gene            complement(3413..4561)
                     /locus_tag="PLP58_10895"
     CDS             complement(3413..4561)
                     /locus_tag="PLP58_10895"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HRJ09069.1"
                     /translation="MIHKPPSCLIVVDALDAPHGTEMFACIVARELHRRGWRLRFHTA
                     HYARKRSAWAGLVTEMEVPVWHPHFWFLTRYELPCMVSAKFFRRVSRKFAPDVIWSPT
                     TDTLAALCLRENAHTHVAPFFVHDPNDASMCPRLPAKWPEVCDLATAMSVHGTRQMQG
                     ARAAYGITRPVEVVWPGSMPPARDPVLPSFDRQPVRFGQFGRLYSMKGALFTVAAFAK
                     CLRAGCHAELHFYGDGPQRGDAWELAASLGIAEKVVFHGSYHHTEMDRLMAEVDVGLM
                     PSLYEGFGLVMLELMARGRPVISTDVGSSKEVLEAYDCGIVTPRADVDALASAMITAA
                     TEPERMRSMSLQARQSWQEHFTPRAFVDRLLAFWGRNGALLTEGEVVA"
     gene            complement(4558..5442)
                     /locus_tag="PLP58_10900"
     CDS             complement(4558..5442)
                     /locus_tag="PLP58_10900"
                     /inference="COORDINATES: protein motif:HMM:TIGR01444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FkbM family methyltransferase"
                     /protein_id="HRJ09070.1"
                     /translation="MTSDTTKNLIRECLEWRAAEHRGALLFYLHKKLPRFKGRERLLH
                     LLALRHARRRLVLVNGRGIRMSINPLEHIGYALCTKQSFEPLSLSLAVRLMAKGGVFL
                     DAGANFGLYSLTMAGIPGARCLAVDALPEAVSLLHKHRFMNPRASMEIFAAALSSLPG
                     LMRLEAPHPGNFGTGRLSESAGADALWVHATTLQVVLCAVGCPAVTLMKMDVEGHEHE
                     ALCGMNLDSSVAPPHLLLEQETGINDGAGIAKVWTLLQGKGYLPFDVLGQPLKPADHP
                     LENNVWWTRARQDVPSCV"
     gene            complement(5465..6142)
                     /locus_tag="PLP58_10905"
     CDS             complement(5465..6142)
                     /locus_tag="PLP58_10905"
                     /inference="COORDINATES: protein motif:HMM:NF016908.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FkbM family methyltransferase"
                     /protein_id="HRJ09071.1"
                     /translation="MLHLDPQCRFLGDSYEPEVWFWLKQRLQPGDVMLDVGAQFGLYA
                     MLAARVTGPFGKIHAFEPAPDSQAVMRRHLKLNNLANSVTLVPAAAGEHSGEALLHMA
                     GTHPSNTLAPTGADPVSLQPVEVRVVSLDDYCRDQRITPRVVKIDVEGWELNVLKGAV
                     ELLAAPGTIFLVEMHPYAWESAGHDAQAVADFLIAYQLELQPLTGQSDPLREYGEVWI
                     KSVSQSD"
     gene            complement(6344..7621)
                     /locus_tag="PLP58_10910"
     CDS             complement(6344..7621)
                     /locus_tag="PLP58_10910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ09072.1"
                     /translation="MFKLLESHQARLATLRRWASLSSQVAGMQMLVQGVSAVTGLLLI
                     RAMEKDAYAWFTLAASMLATLHLLADGGTSTALTSIGGRVYQNAAEFSRLMADGRRMA
                     LRLALIGIVLSAPFFWHLYTRLGAPPVTTAALLGLAIVGMAPAIIATILGVSLRLHSR
                     ARQAQGAELFGACVRLLLCGGLLWVGWADARAMFAGTIFASLCQAWMLRKNTREYTAP
                     PRASTGHQSEITSFVRALYGNHIFFCLQGQIATWLIGWLAGVEQVADLGALSRLAVLF
                     AAFGAPFTHLVAPSMARIQDTHILRRRFALAIGTAFAGTAVLLAAAAWKPGWFLWLLG
                     EKYANLGPELTLALASQGVGMIAGICWGLMVVRGWVSHAWLNILLSCLGYVLGAAFMP
                     LDTVAGVLKMNLLASMPLLCWVLLRCWRRLSIR"
     gene            complement(7643..8515)
                     /locus_tag="PLP58_10915"
     CDS             complement(7643..8515)
                     /locus_tag="PLP58_10915"
                     /inference="COORDINATES: protein motif:HMM:NF016908.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FkbM family methyltransferase"
                     /protein_id="HRJ09073.1"
                     /translation="MLSHLQVLREIWRAPGNQPRRLHAVWRAFRWFLRCHLRAGSGPV
                     LLAVFGARLYPCHTESIIAKHVMYRSEWYDWDLMQFINGYLQQGEHFLDVGANTGLHT
                     LLASTRTSHITCVEPEPSNVQRLRHTLEVNHLDGVTVLALAASDAAGEVCMEGNDVFA
                     RITSPAKNPQTVPAARLDEVLDPQTPVDLCKLDVEGAEWLALRGMTGLMQRGTLPVLA
                     FEHIGHLHAFGQNEGDFLEWLRAQGYTLGVYKHEAHTLDLSEPFTGTDLIAFTQKGRR
                     MIENRLSGMRWLKS"
     gene            complement(8517..9227)
                     /locus_tag="PLP58_10920"
     CDS             complement(8517..9227)
                     /locus_tag="PLP58_10920"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024967.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HRJ09074.1"
                     /translation="MPHDWQSWPSMAPFIEGGTLTGDDGGVEVPHSTSTPNNLTVLTH
                     WHEIIRPRRTLETGLAYGASATLFCELHQRAGISGSEHHAVDPFQQKLWKNCALRHLE
                     SCGLSAGFTHHDGLSAQVLPQLWRQGMSFGMIYLDGSHLFEEVFLDFFYAHHLLEIGG
                     LLAFDDSSHPHVRKVLRFLRRNFHNAYVEHSPFQVTRPRHAWLARIAARLLGRQQLTL
                     FRKTSHFERTWMTPLRDF"
     gene            complement(9389..10381)
                     /gene="rfbB"
                     /locus_tag="PLP58_10925"
     CDS             complement(9389..10381)
                     /gene="rfbB"
                     /locus_tag="PLP58_10925"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: protein motif:HMM:TIGR01181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008460 - dTDP-glucose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HRJ09075.1"
                     /translation="MNLLITGGAGFIGSHVIRSWMDKPEVAKLVNLDCLTYAADLGRL
                     DGIHEQHPSYVFEKSDLRDAAAVRGVVERHGITHVVHLAAETHVDRSITGPGDFLHAN
                     VLGTFHLLEAFRASDFELRISNFVHVSTDEVYGSLGSADDAFTEESPLLPNSPYSASK
                     AAADHLVRSYARTYGFPAAITRCVNNFGPGQHAEKLIPTVLRCLAERRPIPVYGDGLN
                     VREWLPVRDHADALWRVLCAGKSGAVYNIGSGHELANLDLIGRLCDLHDVAHQRPCGE
                     SRQLISFVTDRPGHDRRYAVNSDKIRADLSWHPAQDFDTALRALVLEFKLQLVQ"
     gene            complement(10387..11274)
                     /gene="rfbA"
                     /locus_tag="PLP58_10930"
     CDS             complement(10387..11274)
                     /gene="rfbA"
                     /locus_tag="PLP58_10930"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007413753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008879 - glucose-1-phosphate
                     thymidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HRJ09076.1"
                     /translation="MKGILLAGGSGSRLYPLTRVVSKQLQPVYDKPMIYYPLTVLIAA
                     GIREVCVITTPEDLPRFRQLLGDGTAWGIRIEYREQRQPRGIAEAFLIAEDFIASSNT
                     ALILGDNLFFGGDAFPRAVAEFQEGALVFAYHVQNPRDYGVVEFDAEGRAVSLEEKPA
                     TPRSNYAVPGVYLYDARASAIARALAPSARGELEITDLNREYLRQGKLQVRRLSRGTA
                     WLDAGTSSSLHEASAYVQTIEKRQGMKLGCPEEAALRRGFISLDDFETLASAMPPCEY
                     RDSLRAVSAEVRRVGVVGA"
     gene            complement(11433..11903)
                     /locus_tag="PLP58_10935"
     CDS             complement(11433..11903)
                     /locus_tag="PLP58_10935"
                     /inference="COORDINATES: protein motif:HMM:TIGR04256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GxxExxY protein"
                     /protein_id="HRJ09077.1"
                     /translation="MKPHQNTTMKFAKSADRDSRTYAIIGAAMEVHREMGCGYNELIF
                     QDALEVEFELRGIPCTREQPYHVFYKGHKLPSHLRPDFVCFGEVIVELKALSEITGRE
                     ESQVLGYLKGTGFQTGLLINFGTPSLFHQRFIHSSKWLPEAATERSTDRADFAE"
     gene            complement(12043..13806)
                     /locus_tag="PLP58_10940"
     CDS             complement(12043..13806)
                     /locus_tag="PLP58_10940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009959389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyltransferase C-terminal
                     domain-containing protein"
                     /protein_id="HRJ09078.1"
                     /translation="MIVLGLNAWHGDSAAALVKDGKLVAAAEEERFRRVKHWSGMPAQ
                     AIQWCLKDAGLTLGDVDHIAINRNPKVNNLRRALYVLRKRPSLGLILKRLQNIRRAGG
                     FEDALRRAFPGQPVKARTHHVEHHLAHLASAFHVSGFEEAVCLSVDGFGDFASSAWGM
                     GRGDDIAIDGRVYFPHSLGIFYTVITQFIGFPYYGDEYKVMGLAPYGEPKYLKEMRQI
                     VQVRADGSFRLNLKYFRHHSDDVHYTWDDCIPEVGTLYRKDALESLLGPERAPGAPLE
                     QRHRDLARSAQAMYEEAFFAMLRALHASYQCPRLALSGGCAMNSVANGKVYLNSPFQS
                     LYLPAAAGDAGGAIGSAMVVARQFHNGEPQVANRERFVMDHAYTGPQSSDEEIRALLK
                     TRAADLAAENCATQRCDDETALCQTTAQAITEGKVIGWFQGRMEWGPRALGNRSILGD
                     PRRADMKDILNLKIKRRESFRPFAPSILREHVHEWFEQDDDVPFMMQVFQVREDKRPL
                     VPATTHVDGSGRLQTVHAHTNPRYHRLISAFHALTSVPMVLNTSFNENEPVVCRPEEA
                     LDCFLRTKMDVLVLGDWMIRR"
     gene            14016..14900
                     /locus_tag="PLP58_10945"
     CDS             14016..14900
                     /locus_tag="PLP58_10945"
                     /inference="COORDINATES: protein motif:HMM:NF016777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HRJ09079.1"
                     /translation="MSHPLPRRSVLRLMLACPALAADLRASSSAAADAGESGLVDVNA
                     SLFHWPFRRLPDDEPGLLAARLRKLGFHQAWAGSYEALLHRDPDTVNSRLTDACERHG
                     GGLFRPVGCVNPALPGWEETLRRCAEKHAMHAIRLHPNYHGYTLEDPAFERLLALAAQ
                     AGLLIQLAASMEDTRTQHPLAHVPDVDLRPLPDLLSGHPQARLMLLNHRPAAAVTAPL
                     AALPNVWFDTARTEATDGVARLVALAGRERVVFGSHAPFLIPEAALIRVEEAMLAGLP
                     EADAAALLGANAARLAAR"
     gene            14927..15766
                     /locus_tag="PLP58_10950"
     CDS             14927..15766
                     /locus_tag="PLP58_10950"
                     /inference="COORDINATES: protein motif:HMM:NF016777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HRJ09080.1"
                     /translation="MPAHAIPPWSPMPPPEVLAGFEIWDSYLTQSRGAALMPSLEALM
                     PVVERFALQKLCFFADVGLGTGNAAVEKWAREHSNEIEAPLRRWPDLMLGMIQLNAGD
                     VPASMRALDRWLRDGPMKGVYFPGSAPGGLNCTHPNFDPLVARIHELGGAIMVHTWFK
                     TGEEAGPHGSTPADLVALAARHPRQTFICAHAGGNWELGIQAVRGSPNIVVETSGFDA
                     TASFIEMAARELGAERILFGSHLPSRSLGTELGKVLFADIGEEEKKLILGGNFRRLLG
                     MGR"
     gene            complement(15790..>17828)
                     /locus_tag="PLP58_10955"
     CDS             complement(15790..>17828)
                     /locus_tag="PLP58_10955"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF012403.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HRJ09081.1"
                     /translation="WQPAEDAHPGDGTILYSDRAALAAAQTALHEMRALYDPEFDGFR
                     VRMTKHFPDQFESWARRLPGGVTLTTDERLQTILADPLIARVRIEATQTVDIDWFDLR
                     MIFEVEGAELKAADIRRLIAARGGFVQLADGTWRRVKLELSEEQQALMDQAGLDFDEF
                     SHEAHRLHWRQLAGQGVKEVVNPRAWQMLTQRMEQARLEEKPPVPGGLGVTLRPYQVE
                     GFHFLSYLSLNKFGGILADDMGLGKTIQSISWILWLRGRYRKAWPCLVVCPKSVLDVW
                     ATEFAKAAPEIKVQVLRDKDELDLESLLNEQHVLVLNYAQLRGCVDLLETVKWLAIIL
                     DEGQHIKNPDSKAARAARQLKAENRLVLSGTPVENRLLDLWSLMTFAMPGALGDRKYF
                     NHHFDRRKDGTASERLHARLRPFLLRRTKSQVAKELPSRSEETLLCEMSDAQAARYKE
                     ELARAQQMVITSSGFEVLTRRRFAILQALTRLRQICCHPALADKSATGEESAKLTATL
                     ELIEELHAEGHKVLLFSQFVSMLEILRSRLEDMGVPYYWLTGATQNRAEIVSRFQEDT
                     SPAVFLLSLKAGGSGLNLTAASYVILYDPWWNPAVEAQAIDRAHRIGQTQPVMAYRMI
                     TKGTIEEKIMLLQQKKSLMSSNILGEGGFSRSLDKGDFEFLFGLEAEEAMRRED"
BASE COUNT         3462 a         5435 c         5702 g         3229 t
ORIGIN      
        1 tcggtcgaag gcatcattca ccgccagcca gtccgccgcg cgcgcgggaa ccagcttgtc
       61 ccgcagcact ttccacaatg cgggccaggg atgctcgacg gtcagtgccg catccagacc
      121 gggcaggctg cggctggcca gacgcgcgcg tcccagcatg ccagccaagc atccacccca
      181 ctttcctggc gcgtcatgca gcgagcacaa gaaatcccgc agcaactcct cctcctgcgc
      241 cgccagcgcc agttggaaag cctgatgcac ccccgaatac gccaccgtca cagcatgggg
      301 aacaggcgtc ccgcctgttt gccgttgttt ttccagcatc acgtctattc agcgatcaaa
      361 gctcgaaatt cgaatgggtt tatgagtgcc gatgagtgtt ccatcagtgg ttcaaaaatt
      421 ctcccagagc ggctgaagcc tggacaccaa ctcagcgtcc gcgcacaaac tccagcgccg
      481 cttggatgcc ggccagatcc tcctcgaagc tccagcggtt gatgatctcc aggctcgccc
      541 gccccgcgct ctcgcgcagg gaagcatcca gcaggaaggg ccgcagcacc tccgccagcg
      601 cccgcgcatc cccggcggga aaaaccgctc cattcacccc cggccgcacc agatccggcc
      661 agcagccaac ccgatcactc gcgatcacgg gtttgccagc attcatcacc tcgttcacca
      721 caaggccgaa cggctcataa gtggagggta tgacaaacac atcacacagt tcataaatcg
      781 ccggaagctc cgactggttt ttgaagccgg caaaacgggc ccggccagga atgagatcct
      841 ccgcgagctt ttccaaaacc agccttagtt cgccatcacc taccacaagc agcctggctt
      901 cggcagacgg accggcaaga cttaacgcct gaatcaaagt ggctgcgtct tttttcgcaa
      961 tgagcttgcc acaaaacaaa accaccggca gcccaggctc aagtccaaga ctccggcgca
     1021 agatttcgcg cgacgaactt gcctccctgg cttggcgctg gaagaaggcg ttgtccacag
     1081 cataaggaac ctgaaaaatt ctctcatccg gcaccccata agcacggtag aatgcggcat
     1141 tccaggagcc taccgccagg caggcggcga cgcggcgaaa acgccgggcg taaagccagc
     1201 ggtggacccg ttggtgcaat cctccgccgc gccgacctgg cagagtgctg tcgccgcgaa
     1261 tcatcaacgg cagacgcaac cgcgcggacg cattccaggc cgccaaatcc attggctggc
     1321 tccagccatg cgcccaaaca gccgccgccc cgcattcgcg cgcgtgatcc gccaggagcc
     1381 gggcgagagt ggagcggtct tccgaaggcc ccagcaccaa cgagttgtag ccttccatca
     1441 gcggcctgtc ccaggcgagc tctcgattga actcagggtc aaaaaaacgc tgctctcccc
     1501 gggcataaac caccgaaagc tcaacgcccc cggataccag gcgcctgatc atcgggcttt
     1561 ggtattgaac cgggtgtggc agcgcgtaga ggactttcac agcgatcgag ggcggcgttg
     1621 gaacagcctc cggacagtga tctgcaggcg tgctagcaac gcgtggcggt tgtggtgcat
     1681 gagcatctcc catcggctgc gcatcatgcc gggcagaggc gcggcggcga gaggcctgga
     1741 gacactgtca caagattcct gaagcacctc ggccagcttg aagccgagcc ggcattgaac
     1801 ccgctcccac caatcctgga tctccgcctg tttgcggtcc aatgcctctt tatcgcgcac
     1861 aaacgaccgc atgagaaccg ccgcgtgccg ccaagaagtc acactaggaa ggggatggtt
     1921 tcccaggagc cgggtgaaat aatccagcca cggtctgcgc tcgacaatag gcagcgcgcc
     1981 ggcgtcaagc gcctcataga gacggaaact gtccagattc acatttccca tcggacatgg
     2041 aacgaacaca gactctcgca gcaactccct gtaggctccg ggttccagca tggtctgggc
     2101 agccggcgcg ccggcggtga cacgcaggct gttaggctgc atgtcccgca ggcattccat
     2161 catggaggat cgtgtggccg tcacctggcc tgcaaagctc cacacaaatt ggcgctcaga
     2221 ggaaagccgg ggcttcccac ccatgcgcac atcgcggcac ggcccgagcg ggatctgggt
     2281 gatgccggga agctcaagcg cagtgtgatg gtaactgcga atcacatggc tgaaatgcct
     2341 gtaaacctcc agtggagaac ggaaccactc gtcactgacg tggaaaagta ccacgccaga
     2401 agcctgaccg agacgcttca acaactccgg cctcaagctg cccaaatccg ggcacacgat
     2461 gatgctgttt tccaccacgc gagtttgtcc ggcatccgtc tgcaatgtag cgtcctgcca
     2521 gcccagcagg cgctgcagcc atgccggctc ggcgaaccag ccttcatgat aacaaacaag
     2581 ctcgcgcttc attcaacacc ccccagatga gacatggctc gcatgatgat tggcaaatgg
     2641 aggccccttg cctatccctt ggatctcaaa gccggcgcgg cggagacgat cttgcgcgga
     2701 acctgccgca atgcccagcc gttcctgccg ccacacattt tcctcaaaaa aaacatgcga
     2761 aatattgcct gaatccagca gcgccgtggc tccctcaacg acccaggcgt ccgcaccctc
     2821 gcaatccact ttgagcacgc ggaccggctc gctcacaagg cggtcgagtc tttccgccgg
     2881 aacggagatc gtaccgcctt ccagcccgga ggcgatgcct ccccagccgg tttcttccgc
     2941 gcttcccaga tcaaagctga tccggcccgt ttcacgggtc acagcgcatt ccacgacccg
     3001 cactcgacta gtgagattgt tgcgcgccac attttcccgg agcgcttggg cgtttcttgg
     3061 cgaagcctcg atggcgaccg cgcggttgtc cggcctggcc gcgcaccaca gaagcgtgta
     3121 gtagccgtaa ttggcgccca catccacaag cagaccgcct tgctccttcg cgagcatgtg
     3181 tatggcgcgt gattcatccg cctcgtaaat tccgcagaag gcaatctcac catgaaacga
     3241 gtcggatggt gaaagccgca ttcgaacggt gggttccaac agaagaggcg cgtcctcaaa
     3301 ccatgccgtg caattttttc ggcggggaca aaacacccgg taatacaacg tcgcctttaa
     3361 acgccccggc aggagttgga atagacttcg cttgagcatg gcgggttgaa ttttaggcga
     3421 ctacttcgcc ctcggtcaaa agcgcgccat ttctgcccca aaaggcaaga agtctgtcca
     3481 cgaaagcgcg gggggtgaag tgctcctgcc agctctggcg cgcctggaga gacatgctgc
     3541 gcatgcgctc tggctcggtg gctgcggtga tcatggcgga agcgagtgca tccacatcag
     3601 cgcgtggtgt gacgatgccg cagtcgtagg cttccagcac ctccttgctg ctgcccacat
     3661 ctgtggaaat gaccggtctg ccgcgagcca tcagttccag catcaccagt ccgaagcctt
     3721 catagagcga gggcatcagt cccacatcca cctccgccat gaggcggtcc atctccgtgt
     3781 gatgatagct gccgtgaaag acgacctttt ccgcgatgcc cagactcgcc gcgagttccc
     3841 aggcatcgcc acgctggggg ccgtcgccat aaaaatgcag ttccgcatgg catccggcgc
     3901 gcaggcattt ggcgaacgcg gccactgtga aaagcgctcc cttcatgcta taaagcctgc
     3961 caaactggcc gaagcgcaca ggttgacggt cgaaggatgg aagtaccggg tcccgagcgg
     4021 gtggcatgct acccggccac acgacttcca ccggacgagt gataccgtag gctgcccttg
     4081 ctccctgcat ctgccgggtg ccatgcacgg acatggcagt agcgagatcg cacacttcag
     4141 gccatttcgc aggcaggcgc gggcacatgg aggcgtcgtt agggtcatgc acaaaaaacg
     4201 gcgcgacatg cgtgtgagcg ttttcccgca ggcagagcgc ggcaagcgtg tccgttgttg
     4261 gagaccagat gacatctggc gcgaacttgc ggctgaccct gcggaagaac ttggcggaga
     4321 ccatgcaggg cagctcgtag cgtgtgagaa accaaaaatg cggatgccac accggcacct
     4381 ccatctcagt caccaagccc gcccaagcgg agcgcttccg tgcatagtgc gcggtgtgaa
     4441 aacgcagccg ccagcctctg cggtgcagct cgcgcgccac gatgcaggcg aacatttccg
     4501 tcccgtgcgg tgcgtccagc gcatccacca cgatgagaca ggaagggggc ttgtgaatca
     4561 tacgcaggaa ggcacatcct ggcgggcgcg agtccaccag acattgtttt ccaatgggtg
     4621 atccgcaggc tttaacggtt ggccgagcac gtcaaaaggc aagtagcctt tgccctgtaa
     4681 tagagtccaa actttggcta ttcctgcacc gtcgttgata cccgtctcct gttcgagcaa
     4741 cagatgcggc ggcgctacgg aagagtccaa gttcatgccg catagcgcct catgctcatg
     4801 accctcgaca tccatcttca tcaatgtcac cgcaggacaa cccaccgcgc aaagcacgac
     4861 ttgaagcgtg gttgcatgaa cccacaaggc atccgctccc gctgattcgc tcagtcggcc
     4921 tgtgccaaag ttccccggat gcggggcttc cagcctcatc agtcctggca atgatgaaag
     4981 agcggcggcg aagatttcca tggatgctcg cgggttcatg aagcggtgct tgtggagcag
     5041 gctcactgct tcgggcaagg catccacagc cagacagcgc gcgccgggga tacccgccat
     5101 agtgagcgaa tagaggccaa aatttgcgcc ggcatccagg aagacgccgc cctttgccat
     5161 cagcctgact gccagggata gggagagcgg ctcaaaggac tgctttgtac atagcgcgta
     5221 gccgatgtgt tccaggggat tgatggacat acggattccg cgtccattca cgagaacgag
     5281 cctgcgccgg gcatgccgca gagccagcaa atgcagcagc cgctcccgac ctttgaaacg
     5341 cggaagtttt ttatgaaggt agaaaagcag cgcaccccga tgttccgcag cccgccattc
     5401 cagacattcg cggatgagat ttttggtggt gtcacttgtc atgatgtcaa accgtcaatc
     5461 cgcctcaatc actctgactc acggacttga tccacacctc gccatactcc cgcaaggggt
     5521 cggactggcc ggtcagcggc tgcaactcta attgataagc gatgagaaaa tccgccacag
     5581 cctgcgcgtc gtggccggcg ctttcccatg cataggggtg catctcgaca aggaagatcg
     5641 tgcctggagc tgccagaagt tccaccgctc ctttgagcac gttcaattcc cagccttcca
     5701 catctatctt caccaccctg ggggtaatcc gctgatcccg gcaataatca tccaggctca
     5761 ccacgcgcac ctcgacgggc tgcaaagaaa cggggtccgc gcccgtggga gcgagtgtgt
     5821 tgcttgggtg cgtgcccgcc atgtgcagca aggcctcgcc ggaatgctcg ccggctgccg
     5881 caggcaccag cgtcaccgag tttgcgaggt tgttcagctt taaatgcctg cgcatcaccg
     5941 cctgggaatc cggcgcgggc tcaaacgcgt ggattttgcc gaaaggccct gtcacacgcg
     6001 cagcgagcat tgcgtaaaga ccaaactgcg cgcccacatc cagcatcaca tcaccaggct
     6061 gaagacgctg cttgagccag aaccagacct cgggttcata gctgtcgccg agaaagcggc
     6121 actgtggatc gagatggagc atttgctcgc cattcaactg gcgagggatt cctctgccga
     6181 gtgtcgccat ctcaagagcc catgtgtaga ggcgcgccgg catgctttga ctgccatgcg
     6241 tttcgcctat cccgagcatg cggcgcgtgc agcggagaag tgtttccaat ggatgagtca
     6301 tcgagaagaa ggttggtggc gccagctcaa atcggggtcg cttttacctg atggaaagac
     6361 gcctccagca gcgcagcagc acccagcaaa gaagaggcat ggaggccaaa agattcattt
     6421 ttagcactcc cgccacagta tccagcggca tgaaagcggc acccagcaca tagccgagac
     6481 aggaaagcaa aatgttcagc caggcgtgac tcacccagcc gcgcacgacc atcaagcccc
     6541 agcaaatccc ggcaatcatg cccaccccct gagaggccag tgccagcgtc aattccgggc
     6601 cgagattcgc atacttctct cccagcaacc ataaaaacca acctggcttc cacgctgcgg
     6661 ccgccagcaa aacggctgtg cctgcaaacg ccgtgccgat tgccagcgca aacctcctgc
     6721 gcaggatgtg cgtgtcctgg atgcgggcca tggaaggagc gaccagatga gtgaatggcg
     6781 cgccaaaagc tgcgaagaga acggccagcc gcgagagtgc gccgaggtcg gccacctgct
     6841 cgactccggc gagccagcct atgagccagg tggcgatctg cccctggaga cagaagaaaa
     6901 tgtggttgcc atacaacgcg cgcacaaagc tcgtgatctc gctctggtgc cctgtgctcg
     6961 cccggggagg ggctgtgtac tcgcgcgtat tcttccggag catccaggcc tggcaaaggg
     7021 aggcaaagat cgtgcctgcg aacatggcac gcgcatcggc ccagccaacc cagagcaggc
     7081 ccccgcagag gagaagcctc acacaagctc cgaacagttc cgctccctgt gcttggcgtg
     7141 ctcgcgaatg caatcgcaga gatactccca ggatagtggc tataatggcg ggtgccatgc
     7201 caacaatagc gagaccgagc agagcggccg tcgtcaccgg aggagcacct agacgggtgt
     7261 agagatgcca gaaaaaaggc gctgacagca cgatgccgat cagagccagg cggagggcca
     7321 tgcgccgccc atcggccatc aggcgggaga actcggcagc gttttgatag acccgtccgc
     7381 cgatggaggt aagcgcggtg gaggtgcctc catcggccag caagtgcagc gtggcaagca
     7441 tggaggcggc gagtgtgaac catgcataag cgtctttttc catggcgcgg ataagaagca
     7501 gcccggtgac ggcgctgacg ccctgcacca gcatctgcat tccggccacc tggctggaaa
     7561 gcgaggccca acgccgcagt gtggcgagcc tggcttgatg ggattccagc agcttgaaca
     7621 ttcacgtggg tttccgcagg cttcagcttt tcaaccagcg catcccggac agccggtttt
     7681 cgatcatccg cctgcctttc tgggtgaaag cgatgaggtc ggtgcctgtg aatggctcgc
     7741 tcaaatcgag ggtgtgtgct tcgtgcttat agacaccgag ggtgtacccc tgggcgcgca
     7801 gccattcgag aaagtcgcct tcgttttgcc caaaggcgtg caagtggccg atgtgctcga
     7861 aggccagcac gggcagcgtg ccgcgttgca tgaggccggt catcccgcgc agggcgagcc
     7921 attccgcgcc ttcgacatcc aatttacaaa gatccaccgg cgtttgtgga tcgagcacct
     7981 catcaagccg tgctgcggga acagtttgcg ggttcttggc cggggatgtg atgcgtgcga
     8041 agacgtcatt tccttccatg caaacttctc cagcggcatc cgaggcggca agagcgagca
     8101 ccgtcacacc atcgaggtga ttcacttcca acgtgtggcg cagacgctgc acattgctgg
     8161 gctcaggctc tacgcaggtg atgtgactgg tgcgtgtgga ggcgagcagg gtgtgcaggc
     8221 cggtgttcgc gccgacatcc aagaagtgct caccctgctg gagatagccg ttgatgaact
     8281 gcatcaggtc ccagtcatac cattccgagc ggtacatgac atgcttggca atgatgctct
     8341 ccgtgtggca ggggtaaagg cgcgcgccaa agaccgctag cagcaccggc ccgctgcccg
     8401 cgcgcagatg gcagcgcagg aaccagcgaa aggcacgcca gacggcatga agacgacgag
     8461 gttgattgcc gggggcacgc cagatttcgc gaagaacttg aaggtgggaa agcacgtcaa
     8521 aagtcgcgca gcggagtcat ccatgtgcgc tcaaaatggg aggtcttgcg gaacagggtg
     8581 agttgctggc gtccgagaag acgcgcggcg atgcgtgcca gccaggcgtg ccgggggcgg
     8641 gtcacttgaa agggagaatg ctccacgtag gcgttgtgaa aattccgccg gaggaagcgc
     8701 aggactttgc gcacatgcgg atggctgctg tcgtcaaagg cgagcaggcc gccgatttcg
     8761 agcaggtgat gagcgtagaa gaagtcgaga aacacctcct caaaaagatg gctgccgtcg
     8821 aggtagatca tgccaaagct catgccctgc cgccaaagct gcggcaggac ttgcgcggag
     8881 agcccgtcgt gatgcgtgaa gccggcgctg agaccgcatg actccaggtg ccgcaaggcg
     8941 cagtttttcc agagcttctg ctgaaaaggg tccacggcat gatgctcgct accgctgatg
     9001 ccggcgcgtt ggtgcaactc gcaaaacaag gtggcggagg ctccataggc gagaccggtc
     9061 tccaaggtgc ggcgcgggcg gatgatttca tgccagtggg tgagcacggt gagattgttc
     9121 ggcgtcgagg tggagtgggg gacttccacg ccgccgtcat cgccggtaag tgtgccgccc
     9181 tcgatgaagg gggccatgct gggccaggac tgccaatcgt ggggcatggt ttggggatgt
     9241 gttggagtga agcctttagg cgccacagga gtggactccg ttgccggcac tcatggaaaa
     9301 gtggcttcgc agtggaagtg gccgagccgc gagggcgcga ctcggaacac gcgagggcgc
     9361 gtgtgctccc cgttctttcc tgcgggggtc attgaacaag ctgaagcttg aactccaaca
     9421 ccaaggcgcg cagggcggtg tcgaaatcct gcgctgggtg ccaggaaagg tcggcgcgga
     9481 ttttgtcgga attcaccgcg tagcggcggt cgtgaccggg gcggtcggtg acgaaggaga
     9541 tgagctggcg ggattctccg caggggcgct gatgagctac gtcatgcaga tcgcagagcc
     9601 tgccgatcag gtcgaggttc gccagttcat gcccggaacc gatgttgtaa accgcgccgc
     9661 tttttcctgc gcaaaggaca cgccaaagcg cgtcggcgtg atcccgcacg ggcagccatt
     9721 cgcggacatt gaggccatcg ccgtaaacag ggatggggcg tctttcagcc aggcagcgca
     9781 gcacggtggg gatgagtttt tccgcatgct ggccggggcc gaagttgttc acgcagcggg
     9841 tgatggcggc cgggaaaccg taggtgcgcg cataactgcg cacgagatga tccgcggcgg
     9901 ctttgctggc cgagtagggg gagttgggca aaagcgggct ctcctctgtg aatgcgtcat
     9961 cggcggagcc gagcgagcca taaacctcat cggtgctcac atgcacgaaa ttggaaattc
    10021 gaagttcgaa gtctgatgcg cggaaggctt cgagcaggtg gaaggtacca agcacgttgg
    10081 cgtggagaaa atcacccggg ccagtgatgg agcggtccac atgcgtctcg gcggcgaggt
    10141 ggacgacgtg cgtgatccca tgccgctcga cgacgccgcg cacagcagcg gcgtcacgca
    10201 ggtcggactt ttcgaaaacg tagctcggat gctgctcatg gatgccatcc agcctgccga
    10261 ggtccgccgc ataagtcagg cagtcgaggt tcaccagctt cgccacctcc ggcttgtcca
    10321 tccaggatcg gatgacatgg gagccgatga agcccgcgcc gccggtgatg aggaggttca
    10381 tgattttcac gctcccacga cgcccacacg gcgcacttcg gcggagacgg cgcgcaggga
    10441 gtcgcggtat tcgcaggggg gcatggcgga ggcgagggtt tcgaagtcgt cgaggctgat
    10501 gaagccgcgc cgcagggcgg cttcctcggg gcagccgagc ttcatgccct ggcgtttttc
    10561 gatggtctgc acataggcgg aggcttcgtg caggctgctg ctggtgccgg cgtcgagcca
    10621 ggcggtgccg cggctgagtc ggcgcacctg cagtttgccc tggcgcaggt attcgcggtt
    10681 gaggtcggtg atctccagct cgccgcgcgc ggagggcgcg agggcgcggg cgatggcgga
    10741 cgcgcgggca tcgtagagat agacgccggg cacggcgtag ttgctgcgcg gtgtggcggg
    10801 cttttcctcc aggctgacgg cgcggccttc ggcatcaaac tccaccacgc cgtagtcgcg
    10861 cgggttttgc acatggtagg caaagacgag cgcgccttcc tgaaactcag ccaccgcgcg
    10921 cgggaaggca tcgccgccaa agaaaaggtt gtcgcccagg atgagcgcgg tgttggagct
    10981 ggcgatgaag tcctccgcga tgagaaacgc ctccgcgatg ccgcgcggct gccgctgctc
    11041 gcgatactcg atgcggatac cccatgctgt gccgtcgccg agcagttgcc ggaagcgcgg
    11101 cagatcctcc ggtgtcgtga tcacgcacac ctcgcggatg cccgccgcga tgagcaccgt
    11161 cagcgggtaa tagatcatcg gcttgtcata aaccggctgg agctgcttcg acacgacgcg
    11221 ggtcagcggg taaagccgcg agccactgcc accagctagt agaatgcctt tcatgtccgc
    11281 agatttcatg gggctgtatg gtccgcagat ttcgcggatt tcacagatgg ggcaagggga
    11341 cggagaaacg cgttctcgtc ggagaagatt tgattcttgc catctcgaat ccgcagattt
    11401 ctcgggttgc gcagatgatc agaggtggtc ggttattctg cgaaatctgc gcgatctgtg
    11461 gatcgttcgg tggctgcttc tggtagccat ttggaggaat ggatgaagcg ttggtggaac
    11521 agacttggcg tgccaaagtt gataagcagt ccggtttgaa agccggtgcc tttgaggtag
    11581 ccaaggactt gagattcttc gcggccagtg atttctgaga gtgctttcaa ttccacgatg
    11641 acctcgccaa aacaaacaaa gtcaggccgc aagtgagaag gcagtttgtg gcctttgtaa
    11701 aagacgtggt agggctgctc cctcgtgcag gggatgccac gaagctcgaa ctccacttcc
    11761 aaagcgtctt ggaaaatcaa ctcgttgtaa ccgcagccca tttcacgatg cacttccatg
    11821 gcggcgccaa tgatggcgta ggtccggctg tcacggtccg cagatttcgc gaatttcata
    11881 gtggtgtttt gatgtggttt catgctgttg atggtttgag gttatttgac tgagaaataa
    11941 aatttatccg cagatctcgc ggatttcaca gaggattcag aagcatgcga atgctcgaaa
    12001 tacccgacgg gccgcctcct ctaaccttct gtgaaatctg cgtcatctgc ggatcatcca
    12061 gtcccccagt accagcacgt ccatcttcgt gcgcaggaag cagtcgagcg cttcctcagg
    12121 gcggcacacg acgggttcgt tttcgttgaa gctggtgttc agcaccatgg gcacgctggt
    12181 gagggcgtgg aatgcggaga tgaggcggtg gtagcgcggg ttggtgtggg catggacggt
    12241 ttgcaatctg cctgagccgt ccacatgcgt ggtggcgggc acgagggggc gcttgtcctc
    12301 gcgcacttgg aagacctgca tcatgaaggg cacatcgtca tcctgctcga accactcatg
    12361 cacatgctcg cgcaagatgc tgggggcgaa ggggcggaag gactcgcggc gcttgatctt
    12421 gaggttgagg atgtccttca tgtcggcgcg gcgcgggtcg ccgaggatgg agcggttccc
    12481 cagcgcgcgc ggcccccact ccatgcggcc ttggaaccag ccaatgactt tgccctcggt
    12541 gatggcctgc gcggtggtct ggcaaagcgc ggtttcatca tcacagcgct gcgtggcgca
    12601 gttctcggcg gcgagatcgg cggcgcgggt cttcagcagc gcgcggattt cctcatcgct
    12661 ggactgcggg ccggtgtagg cgtggtccat gacgaagcgt tcgcggttcg ctacttgcgg
    12721 ttcgccattg tggaattggc gcgccaccac catcgccgag ccgatggctc caccggcgtc
    12781 acccgctgcg gcgggcaggt agaggctttg gaagggcgag ttcagataca ctttgccatt
    12841 ggccacggag ttcatggcgc agccgccgga gagggcgagg cgcgggcatt ggtagctcgc
    12901 gtgcagggcg cgcagcatgg cgaaaaaggc ctcctcatac atggcctggg cgctgcgggc
    12961 caggtcgcgg tggcgctgct ccaggggcgc tccaggggcg cgctcggggc cgagcaggga
    13021 ttcgagcgcg tctttgcggt aaagggtgcc gacttcggga atgcagtcgt cccaggtgta
    13081 atgcacgtcg tcactgtgat ggcggaagta tttcaaattc agccggaagc tgccatcggc
    13141 ccgcacctgg acgatctggc gcatctcttt gagatacttc ggctcgccat acggggcgag
    13201 tcccatgacc ttgtattcat ccccataata cggaaagccg atgaactggg tgatgacggt
    13261 gtaaaagatg cccagcgagt gcggaaaata gacacgcccg tcaatggcga tgtcatcccc
    13321 ccggcccatg ccccaggcgg agctggcgaa gtcgccaaag ccatccaccg agaggcagac
    13381 cgcctcctcg aagccggaga catgaaatgc cgaggccaga tgcgccaggt ggtgctccac
    13441 atgatgcgtg cgcgctttga ccggctggcc gggaaaggcg cggcgcagcg cgtcctcgaa
    13501 gccgcccgcg cggcggatgt tttgcaggcg tttgaggatg aggccgaggc tcgggcgctt
    13561 gcgcagcaca tagagcgcgc ggcgcaggtt gttcaccttc gggttccggt tgatggcgat
    13621 gtggtccaca tccccgaggg tgagtccggc gtccttcagg caccactgga tggcctgggc
    13681 gggcatgccg gaccagtgtt tgacgcggcg gaagcgctcc tcctcggcgg cggccacgag
    13741 ttttccgtct ttcacgaggg cggcggcgga gtcgccgtgc caggcgttga gtccgagtac
    13801 gatcatgcgt tttgcgggca agctgccgcc cctggtcgcc tggaaaaacc aggcaccgcg
    13861 cggcatggga aaggttgaat tccgaatttc agaattttcg ggaagcggga ctgtaccatg
    13921 ccggccatgg gcgcaaagag caaactgccc cacgacggca cttgatcggg ctccgttgcg
    13981 aagacagcgg cgcggaagcc gtttgactcc caccgatgtc ccaccccctg ccccgccgct
    14041 cagtgctgcg cctcatgctc gcctgccccg cactggcggc ggacttgcgt gcctcgtcca
    14101 gcgctgccgc tgacgcggga gaaagcgggc tcgtggatgt gaatgccagc cttttccact
    14161 ggcccttccg ccggctgccg gacgacgaac ccggcctgct ggccgcgcgc ttgaggaagc
    14221 tcggcttcca ccaggcatgg gccggcagtt acgaggccct gctgcaccgc gatcccgaca
    14281 ccgtcaactc ccgcctgacc gatgcgtgcg agcgccacgg cggcgggctt ttccgccccg
    14341 tgggctgtgt gaatcccgcc cttcccggct gggaggagac cctgcgccgc tgcgcggaaa
    14401 agcacgccat gcatgccatc cggctgcatc cgaactacca cggctacacg cttgaggacc
    14461 cggcgttcga gcgcctgctc gcccttgccg cgcaggccgg cctgctcatc cagctcgccg
    14521 cctccatgga ggacacacgc acccagcatc cgctggccca tgtgccggat gtggacctgc
    14581 gcccgctgcc tgacctcctg agcggacacc cacaggcgcg cctcatgctg ctcaaccacc
    14641 ggcccgcagc cgccgtcacc gcgcccctcg ccgcgctgcc gaacgtctgg ttcgacaccg
    14701 cgcgcaccga agccaccgac ggagtcgcca ggctggtggc gctggcggga agagagcggg
    14761 tcgtgttcgg ctcgcatgcg ccgtttctca tcccggaggc ggcattgatc cgggtcgagg
    14821 aggcaatgct ggccggtctg ccggaggcgg atgccgctgc cctgctcgga gccaatgccg
    14881 cgcgccttgc cgcccgttga tccaccccaa cccgcgcacc cccgccatgc ccgcccatgc
    14941 cattccaccc tggtcgccga tgcctccgcc ggaggtgctg gcaggcttcg agatctggga
    15001 ctcttacctc acccagtcgc gcggcgccgc cttgatgcca tccctggagg cgctcatgcc
    15061 cgtggtggag cgtttcgctt tgcaaaaact ctgctttttc gccgatgtgg gcctgggcac
    15121 aggcaatgcc gcagtcgaaa aatgggcgcg cgagcactcc aatgaaatcg aagccccgct
    15181 gcgccgatgg ccggacctga tgctgggcat gatccagctc aatgccggcg atgtgcccgc
    15241 gtcaatgcgc gcgctggacc gctggttgcg cgacggaccc atgaagggcg tgtatttccc
    15301 tggttccgca cccggagggc tgaactgcac ccacccgaat ttcgacccgc tcgtggcgcg
    15361 catccacgag ctgggaggcg cgatcatggt gcacacctgg ttcaaaaccg gcgaggaagc
    15421 cggcccgcac ggcagcactc ccgccgacct cgtcgcgctc gccgccaggc atccgcgcca
    15481 gaccttcatc tgcgcgcacg cgggcggcaa ctgggagctg ggcatccagg ccgtgcgcgg
    15541 ctcccccaac atcgtcgtgg aaacctccgg cttcgacgcc accgccagct ttatcgaaat
    15601 ggccgcgcgc gagctcggcg cggagcgcat cctctttggc agccacctgc cctcccgctc
    15661 cctgggcacc gagctgggca aggtgctctt tgcagacatt ggcgaggaag aaaaaaaact
    15721 catcctcggc ggcaacttcc ggcggctgct gggaatgggc cggtgagctt ggggtgctga
    15781 cgcggcaact cagtcctccc tgcgcattgc ctcctcggct tcgaggccga agaggaattc
    15841 gaagtcgcct ttgtcgaggg agcgggagaa gccgccctcg ccgaggatgt tggaggacat
    15901 gaggctcttc ttttgctgca ggagcatgat tttctcctcg atagtgcctt tggtgatcat
    15961 gcggtaggcc atgacgggct gcgtctggcc gatgcggtgg gcgcggtcta tggcctgggc
    16021 ttcgacggcg gggttccacc aggggtcgta gaggatgacg tagctggcgg cggtgaggtt
    16081 caggccgctg ccgccggctt tgagggagag gaggaagacg gcgggtgagg tgtcctcctg
    16141 aaagcggctg acgatctcgg cgcggttttg cgtggcgccg gtgagccagt aatagggcac
    16201 gcccatgtcc tccagccggc tgcggagaat ttcgagcatg gagacgaact ggctgaagag
    16261 caggactttg tgcccctcgg cgtggagctc ctcgatgagc tcgagagtgg cggtgagctt
    16321 ggcgctttcc tccccggtgg cggatttgtc cgcgagcgcg gggtggcagc aaatctggcg
    16381 caggcgggtg agggcctgga ggatggcgaa gcggcggcgg gtgagcacct cgaagccgga
    16441 ggaggtgatg accatctgct gggcgcgggc gagctcttct ttgtaacgcg cggcctgggc
    16501 gtcgctcatc tcgcagagga gcgtctcctc gctgcgggag ggcagctcct tggcgacttg
    16561 ggacttggtg cggcggagca ggaaggggcg caggcgggcg tgcaggcgct cggaggcggt
    16621 gccgtctttg cggcggtcga agtggtggtt gaaatacttg cggtctccga gcgcgccggg
    16681 catggcgaag gtcatgaggc tccagagatc gagcagacgg ttctccacgg gggtgccgct
    16741 gagcacgagg cggttttcgg ccttgagctg gcgggcggcg cgggcggctt tgctgtcggg
    16801 gtttttgatg tgctggccct cgtcgaggat gatggcgagc catttgacgg tctcgagcag
    16861 atccacgcag ccgcggagct gggcatagtt gagcacgagc acatgctgct cgttgagcag
    16921 gctttcgagg tcgagctcgt ccttgtcgcg caggacctgg actttgatct ccggcgcggc
    16981 tttggcgaac tcggtggccc agacgtcgag gactgatttc gggcagacga cgaggcaggg
    17041 ccaggctttg cggtagcgcc cgcgcagcca gaggatccag gagatgctct ggatggtttt
    17101 gcccaggccc atgtcatcgg ccaggatgcc gccgaatttg ttcaggctga ggtaggagag
    17161 gaaatggaag ccctcgactt gatagggccg cagggtgacg ccgaggccgc ctggcacggg
    17221 gggcttttcc tccaggcgcg cctgctccat gcgctgggtg agcatctgcc aggcgcgcgg
    17281 gttgacgact tccttgactc cctgcccggc gagctggcgc cagtgcaggc ggtgggcctc
    17341 gtggctgaac tcgtcaaagt ccagcccggc ctggtccatg agcgcctgct gctcttcgga
    17401 gagctccagc ttcacgcgcc gccaggtgcc gtcggcgagc tggacgaagc cgcccctggc
    17461 cgcgatgaga cggcggatgt cggcggcctt caactccgcg ccctccacct cgaaaatcat
    17521 gcgcaggtca aaccagtcta tgtccacggt ctgcgtggcc tcgatgcgca cgcgggcgat
    17581 gagcgggtct gcgaggatgg tctggaggcg ctcgtcggtg gtgagggtga cgcccccggg
    17641 cagccgccgc gcccaggact cgaactgatc cgggaaatgc tttgtcatcc gcacgcggaa
    17701 gccgtcgaac tccgggtcgt aaagcgcgcg catctcatgc agcgcggtct gcgcggcggc
    17761 gagcgcggcg cggtcgctgt acaaaatggt gccatcgccc gggtgcgcgt cctcggccgg
    17821 ctgccagc
//
LOCUS       DAOMYJ010000089        17809 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Prosthecobacter sp. isolate AMR_MDS_5629
            NODE_1094_length_17809_cov_22.514138, whole genome shotgun
            sequence.
ACCESSION   DAOMYJ010000089 DAOMYJ010000000
VERSION     DAOMYJ010000089.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867850
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prosthecobacter sp. (wastewater metagenome)
  ORGANISM  Prosthecobacter sp.
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Verrucomicrobiaceae; Prosthecobacter.
REFERENCE   1  (bases 1 to 17809)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17809)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.30
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:34:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,742
            CDSs (total)                      :: 4,684
            Genes (coding)                    :: 4,676
            CDSs (with protein)               :: 4,676
            Genes (RNA)                       :: 58
            tRNAs                             :: 55
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17809
                     /organism="Prosthecobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1094_length_17809_cov_22.514138"
                     /isolate="AMR_MDS_5629"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1965333"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(163..3048)
                     /locus_tag="PLP58_10960"
     CDS             complement(163..3048)
                     /locus_tag="PLP58_10960"
                     /EC_number="3.2.1.18"
                     /inference="COORDINATES: protein motif:HMM:NF024488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exo-alpha-sialidase"
                     /protein_id="HRJ09082.1"
                     /translation="MNHNFRILHLSLALLITAATQLGAANPRHWPLDQPRSDALTIHG
                     HAESVAGVQEGALQLDGLSLIELKDSASLHAGGQGLSLTVWVNPFVLSGAQRMIVAKN
                     RYSSSEREWGVMLDRDGKLRLYLWQDGWRTVTAKTTPRTGHWHQVGVVLAREKAELWV
                     NGALEASTVLKSAIPATAAPVTLGGILDGTLKQTFFGALDDVQLFPRALTAQEMAAQY
                     KPVTATLKVPAVPPVASAPKPAGFWSVQAALDAKADRVNLIFDGKSPDRLACDTTLRA
                     MPDGSWVHVMLGGGDREPDPRNGVFLTRSLDQGETWSPMRRLDFGFPRSSDTAATVPS
                     ELMVLDGRCTMFFATHNGKFSGWKEWMIVSEDSCKTWGKPAPAPGRLHDRTFIRNHIV
                     SRDGRILLPFQHYLSGPGPLNPRNGVLISKDGGKTWSEHGNIRLSSSDTYRGWAENNI
                     VELADGRIAMIIRADRLGGVLYYAESNDGGLTWPDFAAKTSIPNPGSKATLYGLGGDS
                     VALLHNPNPLGRYPLALWISFDGMKTWPYQRVLVKESSKGPRAALNYPDGFVSDDKRW
                     LHFAFDDARYRAVRVSARLPEIPVLWDEKQTLPKSADLPVIQGARFSVIKPYEFQKDG
                     YRFLHGLALCFHKGKLYASFGHNQGGENTDTEEARFCVSEDEGRTWSAVKTMDDGGPE
                     VAVSHGVFLSHQGSLWAFMGAYTGTMQGIHTRAYRLNETSGEFEKLGVVVEGGFWPMQ
                     EPIRMGDGNWIMAGISAGGDAPAGGKHPAAVAISHGDNFLKWDLVRISQVPGLGKVWG
                     ESTVIVEGKHITNLSRYGAEAKALTSTSEDYGRTWTLMRPSNLPMATSKPYAGVLSTG
                     QRYLVCSTSADGGGRRAPLTIAVSKLGETTFSKLFVIRRALFPEGPGESHERASLSYP
                     YAIEYNGRLYVGYSNNGGNVGRIGEGRELWNNNSAELAVIPLDALAE"
     gene            complement(3063..4043)
                     /locus_tag="PLP58_10965"
     CDS             complement(3063..4043)
                     /locus_tag="PLP58_10965"
                     /inference="COORDINATES: protein motif:HMM:NF024981.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bile acid:sodium symporter family protein"
                     /protein_id="HRJ09083.1"
                     /translation="MTTGITLIVLFASLAVASGRVKWLAGLGFTFTVLAFGAAAFSFP
                     AWFIAPGGFEMKQIIAPLVQLILLCMGMTLTLGDFARVLSMPRAVIAGAALQYSIMPL
                     AGFAFARLFGLEKEVAAGLILIGSCPGGVSSNVITYLAKGNVPLSVTMTAFSTLLSPF
                     ITPFAMQWLAGAYVPVEVGPMMLSILKMIIAPLAIGFAIRRFVPRVADKLVRALPPLA
                     MLSIAVIVAITVAISRDDLIEVGLILLAASACHNAAGYALGYGAARMLGLDTRDSRTM
                     ALEVGLQNGGMATGLAFNVLHSPAAALAAATFGPWSAITSSMLASWWRKK"
     gene            complement(4216..5124)
                     /locus_tag="PLP58_10970"
     CDS             complement(4216..5124)
                     /locus_tag="PLP58_10970"
                     /EC_number="4.1.3.3"
                     /EC_number="4.2.1.41"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: protein motif:HMM:NF012904.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009436 - glyoxylate catabolic process
                     [Evidence IEA];
                     GO_process: GO:0071704 - organic substance metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HRJ09084.1"
                     /translation="MILPDKVPFGVIAPMTTPFTTEGELNAKSIRSQVEFLVRERADS
                     LAVGGSTGEGQTLDPDELRACVSVTRAAMPADMPLVASTICDSTRQAIRAIKAIEGLG
                     VDAIQLTPVHYLFRPDDEMMIEHFRVIHEETGMPLIIYNVVHWCYLSPELTVRLMNEV
                     PGVMGIKQSAGDLKLFADLMINAPKDRKIYSAVDALMYPSFCLGTPGVIAATPSGIPR
                     ATALLRDAVAKGDHVNARRLHEAILRFWNATDHYNLPACIRYAQSLQGVDAGIPRMPM
                     RPATEEQQARIKPALEAALKLAEELS"
     gene            5275..6108
                     /locus_tag="PLP58_10975"
     CDS             5275..6108
                     /locus_tag="PLP58_10975"
                     /inference="COORDINATES: protein motif:HMM:NF019349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HRJ09085.1"
                     /translation="MMNTAELRRLSELRSLLEGFAARHAASHAASSPEHLKRLRALLK
                     RLTQALRVRDYAAFREADFELHETLIEMAQVSQLKAAWRLAWDGLLHFHGQGFEDYFP
                     DARVLADEHEHLVETIALGDPAAAEDAARSHVEAVWFRVAEKSSAATEPRGDPLQRAT
                     AHLAFRLHSPLKLTDVARKVAFTSAGNLSRLFRQHHGLSFQGYLQKLRLEKAAELLAT
                     THLPVARIAKRVGYRDVSRFGQHFKRRFQTTPAEHRKTQRQPAHEPPDRRSYCAGARV
                     V"
     gene            complement(6553..7491)
                     /locus_tag="PLP58_10980"
     CDS             complement(6553..7491)
                     /locus_tag="PLP58_10980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ09086.1"
                     /translation="MQNLILQPGIRQKIEKRVDKILRDLGNPEPPLRLEEVRDLLRLH
                     VGFYQTDDDGLMQRAIHNLVMAGKQIWARPAILLDVVRKRGIKALVVPDRKRILIDET
                     LHKAKHRWAEGHETIHTVLEWHEPILHGDSEVTMKQSYLDKVEAEANYGAGQLLFLRD
                     RFTAEAVSSAPTVELVKSLAKSFNNTHASTLWRLVETAGRERPILGVMHYHPAPRFAS
                     TKNDPSNPCRHFIRSDAFASMFSATAELAVFECIKSYIRPMSGGPVGSAMVVLTDDNG
                     DEHEFSFETFNFRHECLTLAVHQAKRGKVVFAAQSL"
     gene            complement(7491..7973)
                     /locus_tag="PLP58_10985"
     CDS             complement(7491..7973)
                     /locus_tag="PLP58_10985"
                     /inference="COORDINATES: protein motif:HMM:NF024252.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HRJ09087.1"
                     /translation="MKITVTKEWFRSRAHLEEGHEIGAGSLRHIGKPALSTVPVNTTV
                     ESITFGKTIALMRRSRRWTIDRLASEAETTCEELSEIESNPDCMPEPSTVFSLAKVFH
                     VPPKAFMQKAGLAEAASSRLHEDSIRYAACSGFVEPLTADEEHVLQAVLKALVEHTEL
                     "
     gene            complement(8008..8628)
                     /locus_tag="PLP58_10990"
     CDS             complement(8008..8628)
                     /locus_tag="PLP58_10990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ09088.1"
                     /translation="MEKSDIYLQRSGTREDVDAELWDDISDLHLDLWRSTWVPMIDHA
                     KRRLKSAAVPLHKCPQDLHWDWSEKTDWSRPLLTLQRFAITCGGALQGLMLVNLTKLT
                     ARLPSQKGKDLAYIEFVSTAPWNRPEISGVQQFHGIGINMVRAAVELSRNEGFHGRIA
                     LHSLSQAASFYANTCGMSSLGQDRHYDDLDYFEMTPAQADAFCRPA"
     gene            complement(8633..8845)
                     /locus_tag="PLP58_10995"
     CDS             complement(8633..8845)
                     /locus_tag="PLP58_10995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ09089.1"
                     /translation="MADKRTMTLNLTDAEMTALEDLAMKKDLNKTVVIRQALRLYQMV
                     DQRLREGKKVFIEDEMTKAKSELMLL"
     gene            9068..9706
                     /locus_tag="PLP58_11000"
     CDS             9068..9706
                     /locus_tag="PLP58_11000"
                     /inference="COORDINATES: protein motif:HMM:NF025808.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiubiquitin domain-containing protein"
                     /protein_id="HRJ09090.1"
                     /translation="MPRRKLAARDILDQSGHGQEVVLVRDHETRNDVPFANDAEVDLA
                     EGNVFRVVARCEAGPQPSCTGAAKLAFVSDDEWEATLIGKQTGRSLKRLLGLPDDAVL
                     VRDFESPRDMPIGDDEAVEFRDGAVFACRNRKASRETKIFVNGREKVVAGKTISYAGL
                     VVLAFGTIDPNTIYTVTFKHGPPSKPEGKMSQGDVVKLQCGMHFNVTPTCKS"
     gene            9681..10856
                     /locus_tag="PLP58_11005"
     CDS             9681..10856
                     /locus_tag="PLP58_11005"
                     /inference="COORDINATES: protein motif:HMM:NF004805.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ThiF family adenylyltransferase"
                     /protein_id="HRJ09091.1"
                     /translation="MSHQPANHSPDIFRLRNEGYEVDIRGAHLLISHVPCVNSARQIE
                     FGTLVSKLEFAGDVITKSNDHVAFFIGPHPCHKDGSIMSQIQHSSQTQTLAEGVVVNH
                     SFSNKPPNGYPDYYEKMNRYAEIISAPAQSLDPSVTAKTFRVIEAKPEESVFNYLDTN
                     SSRAEIEAISAKLKGRRIGIVGLGGTGSYVLDLVAKTPVDEIHLFDADTFCQHNAFRA
                     PRAPTVEQLRTPPLKVNYLADIYSRMRRNIIPHSLALNAANVHLLRDLDFVFICMDDG
                     RPKREIVALLEAGAASFVDVGMGVQVGDGNLLGIVRVTTSTPQKRDHFGKCVSFNDAK
                     DDAYSTNIQIAELNMLNAALAVIRWKKLCGFYHDMEHEHDCTYTINGNQLLSDYAQA"
     gene            10837..11259
                     /locus_tag="PLP58_11010"
     CDS             10837..11259
                     /locus_tag="PLP58_11010"
                     /inference="COORDINATES: protein motif:HMM:NF039808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6527 family protein"
                     /protein_id="HRJ09092.1"
                     /translation="MTTLKHKFVECIPGALDEGVLYVSIAHGTAVHRCCCGCGQEVVT
                     PLTPTDWKLIFDGETVSLHPSIGSWNLPCRSHYWITRNCVEWAESWSDWRVTAAAEKD
                     RRLKERFYESLETDTDVADKAARAESRVGFWRRFWKNW"
     gene            complement(11346..11789)
                     /locus_tag="PLP58_11015"
     CDS             complement(11346..11789)
                     /locus_tag="PLP58_11015"
                     /inference="COORDINATES: protein motif:HMM:NF013970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain-containing protein"
                     /protein_id="HRJ09093.1"
                     /translation="MILSADTNLFLYAANPDSPHHEAARRFFEEEIVGERFLLCGLVL
                     VELYMQLRNPAVFKTPKTAKEATAFCDALRANPSWEFGDYEPEVAKPLWNWAAKTTTG
                     FRHIIDARLALTLRHHGVTHFATANAKHFEGFGFERAWSPASPAG"
     gene            complement(11786..12037)
                     /locus_tag="PLP58_11020"
     CDS             complement(11786..12037)
                     /locus_tag="PLP58_11020"
                     /inference="COORDINATES: protein motif:HMM:NF013561.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribbon-helix-helix protein, CopG family"
                     /protein_id="HRJ09094.1"
                     /translation="MTRTQIQFPEPLYQRLKEIAERQDWSLSEVMRKAAEHFVTRFPE
                     EPAPKKVWRFPTLDCGGDFLTDPASLRPEVDAILERSAS"
     gene            complement(12123..12317)
                     /locus_tag="PLP58_11025"
     CDS             complement(12123..12317)
                     /locus_tag="PLP58_11025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ09095.1"
                     /translation="MKYITISMDDGLSFQDRMKATDTEFQRLAREEQELRAELDARHI
                     GLNPANNLTRDELHDRDALR"
     gene            12480..13778
                     /locus_tag="PLP58_11030"
     CDS             12480..13778
                     /locus_tag="PLP58_11030"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialidase family protein"
                     /protein_id="HRJ09096.1"
                     /translation="MIPSFSRSLMVCALLAAQAVFCPAQDPVARTAAGHPAEGELKPF
                     LGAPFLEKQVVFDGDDKVREPYLGVAADGTVLIMRNYAKRLRRSTDGGRTWGEIQEVP
                     FGFLDSNFIVDEMRGELLVIRLWEGEDKLWRSKDSGQSWTEEKITVKPNEVMRWLERT
                     GLKKRGTHGQKELDGWYHLHNNASEAGITLRHGAHKGRLIVTGTFRPLAKEHPSDRKP
                     LDGIHSCAIFSDDGGETWQVSGLFPEATTEEAALVELSDGTLYFNSRSCSGFYDKAFK
                     RELRPDEILRREAWSRDGGNTWEDLRVNQTLIDGGGYDRGYGMKGGLTRLPVKGRDVL
                     IYSNADTAGGAREKLTLWASFDGGQTWPVKRLLNPGHSAYSSLGAARPGTADEGTIYL
                     LFEGGEGGRYTAMQFARLNLSWIVQGGEATGDGTVPDWAR"
     gene            13856..14284
                     /locus_tag="PLP58_11035"
     CDS             13856..14284
                     /locus_tag="PLP58_11035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009958692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5069 domain-containing protein"
                     /protein_id="HRJ09097.1"
                     /translation="MSFPLRSPRDTVGGIMVFGRILDKIRLHARGELPTGWHLGIIAG
                     KRTFDDRVCRLLGVDFDALSRRVLAGGTDDEVLEWCFKNGRRPDDEHIELFNGFMMKR
                     GWRDTASGGLEQQKQEAGLGHRDDIVTFFDLMDAEEGRAP"
     gene            14324..16087
                     /locus_tag="PLP58_11040"
     CDS             14324..16087
                     /locus_tag="PLP58_11040"
                     /inference="COORDINATES: protein motif:HMM:NF015557.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HRJ09098.1"
                     /translation="MEPYLVIGLLACALVLFATEKVSVDVVTLSLLSVLVLTGVLSAE
                     EAFAGFGREVIVMLGSIFVIGAALRETGVLDTLGHLLSRAAGGGTRRLTAGLMCGVGG
                     ISAFMNNTTVVAMFLGPVIGVARRMKVPPSRLLMPLAYASILGGTCTLIGTSTNVAVS
                     GALKQAGHGELGMFDMTPVGVMICAAGLLYMMLAGIHLIPERDKSAQAEGAANLREYL
                     SEVVILQGSPLIGQEIFSSDFSVLGFQVIHILRHGLELDLGPHSRFEEGDVVRVAGKV
                     ENLIRIKKIEGLDIMEDVSLRESGVDMRGASLAEVVLTPRSSLVGRTLRTSNFRRRSG
                     LSVLALLRGDQSLKDKMADVVLEAGDLLLVQGPYDRLKTYEENSEMVVITEHAWEISA
                     RKRGYALLGVFGLVVLASSAGWVPAAAGFLIAALFALLARCITLETAYENIDWRLLIL
                     IGGMTAFGTAMTNSGAAEMLAGFITGGLQSWGPHAVLAGFCVLTVLLTQGLSNAAAAL
                     VVLPIALRSAESLAVSPVSFGVAVMLSASISVLTPFEPSCILVYGPGKYRFADFVKVG
                     SGLVLCSLAIIIVMVPVLWPF"
     gene            complement(16097..16552)
                     /locus_tag="PLP58_11045"
     CDS             complement(16097..16552)
                     /locus_tag="PLP58_11045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009959827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="R3H domain-containing nucleic acid-binding
                     protein"
                     /protein_id="HRJ09099.1"
                     /translation="MTALTHARQILDSMLGYLGFIAKVDEVEGPEGPTLQVHTEDSKL
                     LIGRHGATMEDIQYLVNRILQKHLPDAPRIRVDIGYFRSMKEDKIVEHAREMGERVRS
                     TGQSQMLDPMNSYYRRMVHNAFADDPDVMSVSVESGSDRFKRILLKRRG"
     gene            complement(16648..>17809)
                     /locus_tag="PLP58_11050"
     CDS             complement(16648..>17809)
                     /locus_tag="PLP58_11050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009964933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRJ09100.1"
                     /translation="SSAMNSLLHDNVAGMRQIKAYAMEREEKDRFNEASGALRKATLH
                     VMRVWAVYRPGMHFLTSAGMVIVLWQGAHGLMEGRIEKGDLAAFLLLLKFFYDPVEQL
                     HQLNQILQSGRAAGERVFGILDEEPESDTAGGRELGPVTGHVRYENVGFSYGGSIPTV
                     HGITLEAKPGETVALVGPTGAGKSTLINLLTRFYEYDEGVVTIDGTPVHEVNKASLRR
                     SIGYVTQESFLFNGTLRENLLIGKRDATDEELWQALASANADAFVRRLEHGLDSRVGE
                     RGIKLSVGEKQRVSIARALLRNPPILLLDEATASVDTETERQIQAALEKLMENRTSFV
                     IAHRLSTVRHAHRIYVLDQGRIVEQGTHEELVEKDGLYAGLCRTSLMEARLP"
BASE COUNT         3256 a         5380 c         5712 g         3461 t
ORIGIN      
        1 gggcgggcgg gcaatggcgt cgagagtcat ggaagggtca tcaggtggtc agaccaaaac
       61 gggaagcctg atcacatcta tcacgactgc gctgccgcga agtggcgtgt gtccgccatc
      121 acggcggctg attctttatt gtgaggcgcc gcagtggcga agctactcag ctaatgcatc
      181 aagcggaatc accgccagct cggcgctgtt gttgttccac agctcgcggc cttcaccgat
      241 gcggccgacg ttgccgccgt tgttcgagta gccgacgtaa aggcggccgt tgtactcgat
      301 ggcgtaggga tacgaaagcg aggcgcgctc gtggctctcg ccggggcctt cggggaagag
      361 cgcgcggcgg atgacgaata atttgctgaa cgttgtctcg ccaagctttg acacggcgat
      421 ggtcaagggg gcgcgacgtc cgccgccgtc ggcgctggtg ctgcacacga ggtagcgctg
      481 gccggtgctc aagacgcccg cgtagggctt gctcgtggcc atgggcaggt tgctcgggcg
      541 catgagcgtc caggtgcggc cgtagtcctc gctggtggag gtgagggcct tcgcctcggc
      601 tccgtagcgg cttaggttgg tgatgtgttt gccctcgacg atcacggtgg actcgcccca
      661 gaccttgccg aggccgggca cctgcgaaat gcgcacaagg tcccatttca gaaaattgtc
      721 gccgtggctg atggcaacag cggcgggatg cttgccgcct gcgggcgcgt cgcccccggc
      781 ggagatgccc gccatgatcc agttgccatc gcccatgcgg atcggctcct gcatgggcca
      841 gaagccgcct tcgaccacca cgccgagctt ctcaaactcg ccgctcgttt cattcagccg
      901 ataggcccgg gtgtggatgc cttgcatcgt gccggtgtag gcccccatga aggcccacaa
      961 cgagccttgg tgggagagga acacgccgtg gctgacggca acctccggtc cgccgtcgtc
     1021 catcgttttc accgcgctcc aggtgcgccc ttcatcctcg ctgacgcaaa agcgtgcctc
     1081 ctcggtgtcg gtgttctcgc cgccctggtt gtgaccaaag ctggcgtaga gtttgccttt
     1141 gtggaagcaa agcgcgaggc cgtgcagaaa acggtagccg tccttctgaa actcgtaggg
     1201 tttgatgacg gagaagcgcg cgccttggat gacgggcagg tcggcggact tgggcagggt
     1261 ttgtttttca tcccagagca cgggaatctc cggcaggcgc gcgctcactc gcacggcgcg
     1321 gtagcgggcg tcgtcgaagg cgaaatgcag ccacctttta tcgtcgctga caaagccatc
     1381 gggatagttc aaggcagcac gcggaccttt gctcgactcc ttcaccagca cgcgctgata
     1441 gggccaggtc ttcatgccgt caaagctgat ccacagtgcc agcgggtagc ggccaagcgg
     1501 attgggattg tgcagcagcg ccacgctgtc gccaccgaga ccgtaaagcg tggccttgga
     1561 gccggggttc gggatgctcg ttttggcggc gaagtccggc caggtcaaac cgccgtcatt
     1621 cgactcggcg taatagagca caccgccgag gcggtcggcg cggatgatca tggcaatgcg
     1681 gccatcggcg agttcgacga tgttgttctc cgcccagccg cgataggtgt cgctgctgga
     1741 gaggcggatg ttgccgtgct cgctccaggt tttaccaccg tccttgctga taagcacgcc
     1801 gttgcgcgga ttgagcgggc cggggccgct gaggtaatgc tggaaaggca aaaggatgcg
     1861 gccatcgcgg gaaacgatgt gattgcggat gaaggtgcgg tcatgcaggc ggcctggcgc
     1921 gggagcgggt ttgccccacg tcttgcagga gtcctcgctg acgatcatcc attccttcca
     1981 gccgctgaat tttccgttgt gggtcgcgaa aaacatcgtg cagcgcccgt cgagcaccat
     2041 gagttcgctc ggcaccgtcg cggcggtgtc gctggatcgc ggaaagccga agtcaagccg
     2101 ccgcatgggg gaccaagttt cgccctggtc cagactgcgg gtaaggaaga cgccgttgcg
     2161 cggatcgggt tcccggtcgc ctccgccgag catgacatgg acccaggacc cgtcgggcat
     2221 ggcacgcagc gtggtgtcgc aggcgagccg gtccggactt ttgccgtcga agatgaggtt
     2281 gacccggtct gccttggcat caagcgctgc ctgtacgctc caaaacccgg ccggcttggg
     2341 tgccgaggcc acaggcggca cggcgggcac tttcagggtg gcggtcacgg gtttgtactg
     2401 cgcggccatt tcttgcgcgg taagtgcgcg ggggaaaagt tgcacgtcgt ccagggcgcc
     2461 gaaaaacgtc tgcttgagag tgccgtccag aatgccgccg agtgtgaccg gtgcggcggt
     2521 ggcaggaatg gcagacttca acacggtgct ggcctcgagg gcgccattga cccagagttc
     2581 cgccttttcc ctggcgagta cgactccgac ctgatgccag tgtccagtcc ttggtgtggt
     2641 cttcgctgtc actgtccgcc agccatcctg ccacagatag agacgcagct tgccgtcccg
     2701 gtccagcatc acgccccact cgcgctcgct gctggaataa cggttcttcg ccacgatcat
     2761 ccgctgcgca ccgctcagca caaagggatt cacccacacc gtgaggctga ggccctgacc
     2821 gcccgcatgc aaactcgcgg agtccttcag ttcgatcaag gaaaggccat cgagctgcaa
     2881 ggcgccttcc tggacacccg ccacgctttc ggcgtggccg tgaatggtca aagcatcaga
     2941 cctgggctga tcgagcggcc aatgacgtgg atttgcagcg ccaagttgag tggctgctgt
     3001 aatgagcaga gcgagagaga gatgaaggat gcggaagttg tggttcataa attggctgga
     3061 ttttatttct tcctccacca cgaagccagc atagagctgg tgatggcgct ccacggacca
     3121 aaggtggcgg cggcgagagc ggcggcgggg ctgtgcagaa cgttgaaggc gaggccggtg
     3181 gccatgccgc cgttttgcaa gccgacttcg agcgccatgg tgcggctgtc gcgggtgtcg
     3241 agaccgagca ttcgcgccgc gccgtagccg agcgcgtaac cggcggcgtt gtggcaggcg
     3301 gaggcggcca gcaggatgag gccgacttcg atcaagtcat cgcgcgagat cgccacggtg
     3361 atggcgacga tgacagcgat ggacagcatc gccagcggcg gcagcgcacg gacgagcttg
     3421 tccgccacgc gcggaacgaa gcggcggatg gcaaaaccga tcgccagcgg cgcgattatc
     3481 attttcagaa tggacagcat catcggcccg acctcgaccg gcacgtaggc ccccgcgagc
     3541 cactgcatgg caaagggcgt gatgaaaggc gagagcagcg tcgagaaggc ggtcatcgtc
     3601 acggagagcg gcacattgcc tttcgccaga taggtgatga cattggacga cacgcctccc
     3661 ggacacgagc cgatgagaat cagacccgcg gcgacttctt tttccaagcc aaaaagccgc
     3721 gcaaaggcga aacccgccag cggcatgatg ctgtattgca aggccgcgcc tgcgatcacc
     3781 gcgcgtggca tcgaaagcac gcgggcgaag tcccccagtg tgagcgtcat gcccatgcac
     3841 agcaggatga gctgcaccag cggcgcgatg atttgcttca tctcaaatcc gcccggcgcg
     3901 atgaaccacg ccggaaagct gaacgccgcc gcgccgaagg ccagcacggt gaaggtgaag
     3961 ccaagacccg caagccactt cacacgaccc gacgccaccg cgagcgaggc gaagagcacg
     4021 atgagtgtga tgccggtggt catgggattg atccttggac tgcggcagcc tgctgccgct
     4081 ttggagtggt ggcagccctg ctgccggggg tgggtttacg ttgtcagtat aggtgggtca
     4141 ttcggactgc gacggccagc aggctggcct cgggaaagcg gcagcaggct gccgcagtcc
     4201 agggcgcttc gcgctttagg acaactcctc cgccagcttc agcgcggctt cgagcgcggg
     4261 cttgatcctc gcctgctgct cctcggtggc cgggcgcatg ggcatgcgcg ggatgccggc
     4321 gtccacaccc tgcaaggact gcgcataacg aatgcaggca ggcaggttgt aatggtcggt
     4381 ggcgttccaa aaacgcagga tggcctcgtg caggcggcgg gcgttgacat ggtcgccctt
     4441 cgccacggca tcgcgcagca gcgcggtggc gcgcgggatg ccggagggcg tggcggcaat
     4501 gacgccggga gtgccgaggc agaaggacgg atacatcagc gcatccacgg cgctgtagat
     4561 cttgcggtcc ttcggcgcgt tgatcatgag gtcggcgaag agtttcaaat cgcccgcgct
     4621 ctgcttgatg cccatcacgc ccggcacctc gttcatcagg cgcacggtta gctccggcga
     4681 gagatagcac caatgtacga cattgtagat gatgagcggc atcccggtct cctcatggat
     4741 cacgcggaag tgctcgatca tcatctcgtc gtccgggcgg aagaggtaat gcaccggcgt
     4801 gagctgaatg gcgtccacgc cgaggccctc gatggccttg atggcgcgga tcgcctgccg
     4861 cgtgctgtcg caaatcgtgc tggcgaccaa aggcatgtcc gccggcatgg ccgcacgggt
     4921 tacgctgacg caggcgcgga gttcatcggg gtccagcgtc tgaccttcgc cggtggaacc
     4981 gccgaccgcg aggctgtccg cccgctcgcg caccaggaac tccacctggc tgcggatgga
     5041 cttggcgttc agttcgccct cggtggtaaa gggcgtcgtc atcggcgcga tgacaccaaa
     5101 agggactttg tcgggtagga tcatggcagg aagtcgtgtg gttgatttgg cataatggaa
     5161 tgcctgtggc gtgatgaaac ggcgccgttt ccacgttttc aatgctgccg ttccagcggc
     5221 ggtcgccttt gaccgctgcc gcatagcctg ctgctcgatt tcgcctattt gcccatgatg
     5281 aacaccgccg aactgcgccg cctctcggaa ctccgctcgc tgctggaagg ctttgccgcg
     5341 cgccatgcgg ccagccacgc cgcaagctcg ccggagcatc tcaaacgcct gcgcgccctc
     5401 ctcaagcgtc tgacccaggc gctgcgcgtg cgcgattacg cggcctttcg cgaggccgat
     5461 ttcgaactgc atgagaccct catcgagatg gcgcaagtct cccagctcaa ggcggcctgg
     5521 cggctcgcct gggacggcct gctgcatttt cacgggcagg gctttgagga ctacttcccc
     5581 gatgcgcgcg tgctggccga cgagcacgag catctcgtcg aaaccatcgc gttaggagat
     5641 cccgctgccg ccgaggacgc cgcgcgcagc catgtcgagg ccgtatggtt tcgggtggct
     5701 gaaaagtcca gcgccgccac cgagccgcgc ggcgatcctt tgcaacgcgc caccgctcac
     5761 ctcgccttcc gcctgcattc accgttgaag ctgacggacg tggcgcggaa ggtggccttt
     5821 accagcgcag gcaatctcag ccgtctcttc cgtcagcacc acggcctcag cttccagggc
     5881 tatctgcaaa agctccgcct cgaaaaggcc gccgaactgc tcgccaccac ccatctcccc
     5941 gtcgcccgca tcgccaaacg cgtcggctac cgcgatgtct cgcgcttcgg ccagcatttc
     6001 aaacgcaggt ttcagacaac tcctgcggag catcgaaaaa cccagcgcca gcctgcccat
     6061 gagccgcccg acagaagaag ctactgcgcg ggagcgcgcg tggtttgagg agcatctcaa
     6121 aggggcgggc gcatccacag atcgcttaac ttcctctcac accgcgcctt cgaaaaatcc
     6181 acccaactca actaacccca tctttgctgt ccgctttttc gaagaaagat caggtccacg
     6241 cctgcggccg tcaatccctg accctcgcca caaatcgccg cccgcagcgt gccgtcgaag
     6301 gatcatgtct tcaaagacat caccccctgc ggcatctact gcgaaggttt ccaggaagcg
     6361 accacgctgg agatcgcgtg gtttgaggag catttgaaaa ggaagtgaag agttttctcg
     6421 ttgctggtca gccgcagtca agcggagcgg cccggcaaaa ctgtggcgtg atggagacag
     6481 cgcgggcaaa gcgacgaaat caaaagaggc ggggacgaac acaaggtgcg agcggcccgc
     6541 cggaactcgc catcatagcg attgcgctgc gaagacaact ttgccgcgct tcgcttggtg
     6601 aacggcgagc gttaggcatt cgtgccggaa attgaaggtc tcgaaagaga actcgtgttc
     6661 gtcgccattg tcgtcggtaa gcacaaccat tgcggaacca accgggccgc cactcatggg
     6721 acgaatgtag ctcttgatgc actcgaacac ggcaagctcc gccgtggccg agaacatgga
     6781 agcaaaggcg tccgaacgga tgaagtggcg gcagggattc gaggggtcat tcttggtgga
     6841 ggcgaaacgc ggggcgggat ggtagtgcat cacgccgagg atggggcgtt cgcggccggc
     6901 tgtttcgacc aagcgccaaa gtgtgctggc gtgggtattg ttaaaggact tggccaacga
     6961 tttcacgagt tccacagttg gtgctgagga gactgcctcg gcggtgaagc ggtcgcgcag
     7021 gaaaagcagt tggcccgcgc cgtagttggc ttccgcttcc actttgtcga ggtacgattg
     7081 cttcattgtc acctcgctgt cgccatgcag gattggctcg tgccattcga ggacagtgtg
     7141 gatggtctcg tggccttcgg cccagcggtg ctttgccttg tggagcgttt catcaatcaa
     7201 gatgcgcttc ctgtcgggca cgaccagcgc cttgatgccg cgcttccgca cgacatcaag
     7261 caggatggcc gggcgagccc aaatctgttt tccagccatc acgaggttgt ggatggcgcg
     7321 ttgcatcaat ccgtcgtcgt ccgtctggta aaaaccgacg tgcagtcgca gtaaatcgcg
     7381 cacctcctcc agccgaagcg ggggttccgg gttaccgagg tcgcgcagga ttttgtccac
     7441 acgcttctcg attttctgcc ggatgcccgg ctggaggatg aggttttgca tcagagttca
     7501 gtgtgttcga ccagcgcctt gagcaccgct tgaagcacat gctcctcgtc tgctgtgagt
     7561 ggctccacaa acccggaaca cgccgcgtag cgaatgctgt cctcgtggag acgggaggat
     7621 gcagcctccg ccagcccggc tttctgcatg aacgccttcg ggggcacgtg gaacaccttg
     7681 gcgagactga acacggtgct cggttccggc atacagtcgg ggttggactc aatttcagac
     7741 agttcctcgc aagtggtctc cgcctcactg gcgagccggt caatggtcca gcgacgtgaa
     7801 cgccgcatca gggcgatggt cttgccgaac gtgatgcttt cgactgtggt gttgactggc
     7861 actgtgctca gcgcgggctt gccgatgtgg cgcagtgaac cggccccgat ttcgtgtccc
     7921 tcttcgagat gtgcgcggct gcggaaccat tctttggtaa cggtgatttt catgggatgt
     7981 gcaggttggt gtggtggaaa gtgaggctca cgctggacgg cagaaggcgt cggcctgggc
     8041 aggggtcatt tcaaagtaat caaggtcgtc atagtgccgg tcttggccga gcgaggacat
     8101 gccgcaggtg ttggcataga aactcgccgc ctgactcagc gaatgaagtg cgatgcgccc
     8161 gtgaaatccc tcattacggc tcagttcaac ggctgcgcgc accatgttga tcccaatgcc
     8221 gtggaactgt tgaactccgg agatttccgg gcgattccat ggagcggtgg agacgaattc
     8281 gatatacgca aggtctttgc ctttctgcga aggcaagcga gccgtcagct tggtgaggtt
     8341 caccagcatc agcccttgca acgcgccacc acaagtgatg gcaaatcgct gtaatgtgag
     8401 aagggggcgg ctccagtcag tcttttcact ccagtcccag tgcaagtcct gaggacattt
     8461 gtgaagtggc acagcggcac ttttcagtcg gcgcttggcg tggtcaatca tcggaaccca
     8521 agtgctccgc caaaggtcga ggtgcaggtc gctgatgtcg tcccaaagct cggcatccac
     8581 atcttcgcgg gtgccagagc gttggaggta aatgtcggat ttttccatcg gttcagagca
     8641 gcatgagttc ggatttcgcc ttggtcattt cgtcttcgat gaagaccttt ttcccctccc
     8701 ggaggcgctg gtccaccatc tggtaaaggc ggagcgcctg ccggatgacg accgttttgt
     8761 tgaggtcttt tttcatggca aggtcttcca gcgcggtcat ctccgcgtcg gtcagattca
     8821 gggtcattgt gcgtttgtcg gccataggat atgcattgcg tgtggatacg ataagcacag
     8881 aacatggaac gcgtccacaa attttccaca ttttagttga cagtatggct gttaaatggc
     8941 tatttaatgg cctatggata ctcattccga gaaccaacaa ggccacggca ccgccggggc
     9001 agaacatgtg caaaattgtg gctccgcacc caagtgggcc gcgctcatca acgatcggct
     9061 cttcccgatg ccccgccgca aactggccgc gcgggacatc ctcgaccagt cgggccacgg
     9121 gcaggaggta gttctcgtcc gggaccacga gacgaggaac gacgttccct ttgcaaacga
     9181 cgcggaagtg gacctcgcgg agggcaatgt cttccgagtg gttgcccgtt gcgaagcggg
     9241 accacagcca tcctgcaccg gcgcggcgaa gctggcgttt gtgagcgatg acgaatggga
     9301 agcgacgctc atcggcaaac agacgggccg ctcgctcaag cggctgcttg gcttgccgga
     9361 tgacgcggtg cttgtgcgag acttcgagtc tccgcgcgac atgcccatcg gtgacgacga
     9421 ggcagtcgag ttccgcgatg gggcggtgtt cgcctgccgc aacaggaagg cttcccgtga
     9481 gacgaagatt ttcgtcaacg gacgcgagaa ggtcgtcgcc gggaagacca tctcgtatgc
     9541 cggcctagtg gtgctggcgt tcggcaccat tgacccaaac acgatttaca cggtcacgtt
     9601 caaacacggg ccgcccagca agcccgaagg caaaatgtcc caaggcgatg tcgtgaaact
     9661 ccaatgcgga atgcacttca atgtcacacc aacctgcaaa tcatagtcct gacatcttca
     9721 ggcttcgcaa cgagggctac gaagtggata tcaggggggc gcacctcctg atttcccacg
     9781 tcccctgcgt caactcagcg aggcaaattg agttcggtac tttggtgtcc aaactcgaat
     9841 ttgccgggga cgtcatcacc aagtcgaacg accacgttgc tttcttcatc ggcccgcatc
     9901 cgtgccacaa ggacggcagc atcatgtcgc aaatccagca tagcagtcag acgcagaccc
     9961 tggcggaggg agtcgtcgtg aaccactcgt tctcaaacaa gccgccgaac ggctacccgg
    10021 actactacga gaagatgaac cgctacgcgg aaatcatctc ggcaccggcg caatcgctcg
    10081 acccatcggt gacggcgaag acgtttcgag tgattgaggc aaagccggag gaatccgtct
    10141 tcaattacct cgacaccaac tcaagccgcg cggaaatcga agccatcagc gccaagctca
    10201 aagggcggcg catcggcatc gtcggcttgg gtggcaccgg ctcctacgtg ctcgacctcg
    10261 tggcgaaaac gccggtggat gaaatccatc tcttcgacgc ggacacgttt tgccagcaca
    10321 acgccttccg tgcgccgaga gcgcccacgg tggagcaact ccgcacgcca ccgctgaagg
    10381 tgaactacct cgctgacatc tactcgcgga tgcgccgtaa catcattcca cactcattag
    10441 cactgaacgc ggcgaacgtg catcttctcc gggacttgga tttcgtcttc atctgcatgg
    10501 acgacgggag gcccaaacga gaaatcgtcg cgctgctgga ggctggcgct gcttcgttcg
    10561 tggatgtcgg catgggtgtc caagttggcg atgggaacct gctcggaatc gtccgtgtga
    10621 cgacaagcac gccgcagaag cgcgaccatt tcggcaagtg cgtatccttc aacgacgcca
    10681 aagacgacgc ctactccacg aacatccaaa ttgccgaatt gaacatgctc aacgccgcgc
    10741 tcgccgttat ccgctggaag aagctctgcg gcttctatca cgacatggag catgagcacg
    10801 attgcaccta caccatcaac gggaaccaac tactcagtga ctacgctcaa gcataagttc
    10861 gtcgagtgca tccctggcgc tctggacgaa ggcgtgctct acgtgtccat cgcgcacggc
    10921 accgccgttc accggtgctg ctgcggctgt ggccaggagg tcgtgacgcc gctcacgccg
    10981 acggattgga agctcatctt cgacggcgaa accgtgtcgc tgcatccatc catcgggagc
    11041 tggaaccttc cgtgtcgctc ccattactgg attacgcgga actgcgtcga atgggcggaa
    11101 agctggtcag actggcgcgt aactgccgca gcggaaaaag accgcaggtt gaaggagcgg
    11161 ttttacgaaa gtctcgaaac cgacaccgac gtggcggaca aagctgcccg tgccgaaagt
    11221 cgcgtgggat tttggcggcg attctggaag aactggtagt gctggcaccg cctccaggtg
    11281 ttcggattgg tggggcgtat tccgctggtg cgcttgtgtc tcgctttagc tccagctact
    11341 ttccgttagc ccgccgggct agctggactc cacgccctct caaacccaaa gccctcgaag
    11401 tgcttggcat tggccgtggc aaagtgggtg acgccgtggt gccgaagggt gagggcgaga
    11461 cgggcgtcga tgatgtggcg gaagcccgtg gtggttttgg cggcccagtt ccagagcggc
    11521 ttggcaacct cgggttcgta gtcgccaaac tcccaggagg ggttggcgcg gagagcgtcg
    11581 cagaaggccg tcgcttcctt ggcggtcttg ggcgtcttga acaccgctgg attgcggagc
    11641 tgcatataaa gctccaccag cacgaggccg cagagcagga atcgctcacc gacgatctcc
    11701 tcctcgaaga agcggcgggc ggcttcgtga tgcggcgaat ccggattggc cgcgtagagg
    11761 aagaggttgg tgtcggcgga gaggatcatg atgcgctgcg ctccaggatg gcatccactt
    11821 cggggcgcag gctggcggga tcggtgagga agtctccgcc gcagtcgagg gtagggaaac
    11881 gccagacttt cttgggcgct ggctcctccg ggaagcgggt gacgaagtgc tccgccgcct
    11941 tgcgcatgac ctccgaaagc gaccaatcct gccgctcggc gatctctttg agccgctggt
    12001 agaggggctc agggaactga atctgagtgc gtgtcatgat gcgttttata catcacgcgc
    12061 agatggctgt caatttggcg gcggcgagga tgaaggcgtc ccacaatctc ctgtttttgg
    12121 tatcaacgaa gcgcatcgcg gtcgtggagt tcgtctctgg tgaggttgtt ggcgggatta
    12181 agcccgatgt ggcgggcatc gagttcggcc cgaagctctt gctcctctct ggcaagtcgc
    12241 tgaaactcgg tatcggtagc tttcatgcgg tcctggaagg aaagcccgtc atccatgctg
    12301 atggtgatgt atttcatggc ttgggcagtt tcttgcagtt tcacagttca ggcaagcccg
    12361 tggttttctc agccaagcgg actcaccacc tgtcatgtgg gtccctttca tcgctgagcc
    12421 ggttcgttca aggcctcgaa cgcaggatgg gcatccttta gcgcgtaatg tctggtacga
    12481 tgattccttc tttctcccga tccctgatgg tgtgcgcgct gctggcggca caggcggtat
    12541 tttgcccagc gcaagatcca gtcgcgcgca cggcggcggg gcatccggcg gagggggagc
    12601 tgaagccatt tctcggcgcg ccgtttttgg agaagcaggt cgtctttgat ggcgatgaca
    12661 aggtgcgcga gccttacctg ggcgtggcgg cggatggcac ggtgctgatc atgcgcaact
    12721 atgcgaagcg gcttcgccgt agcacggatg gcgggcgcac ctggggcgag atccaagagg
    12781 tgccgttcgg gtttctcgac agcaatttca tcgtggatga aatgcgcggc gagctgctgg
    12841 tgatccgcct gtgggagggc gaggacaagc tctggcgcag caaggacagc ggccaaagct
    12901 ggacggagga aaagatcacg gtgaagccca acgaggtgat gcgctggctg gagcgcacgg
    12961 ggctgaagaa acgcggcacg cacggccaga aggagctgga tggctggtat catttgcaca
    13021 acaatgccag cgaggcgggc atcacgctgc ggcacggtgc gcacaaggga cgcctgattg
    13081 tgaccggcac ttttcgtccg ctggcgaagg agcatccctc cgaccgcaag ccgctcgatg
    13141 gcatccatag ctgcgcgatt ttcagcgatg atggtggcga gacctggcag gtgagcggtc
    13201 tttttcccga ggcgaccacc gaggaggcgg cgctggtgga gctgagcgac gggacgctgt
    13261 atttcaactc gcgcagctgc agcggctttt atgacaaggc tttcaaacgc gagctgcgac
    13321 ctgatgagat cctgcgtcgc gaagcgtgga gccgcgatgg cggcaatact tgggaggatc
    13381 tgcgggtgaa tcaaacgctg attgacggcg gcggctatga tcgcggctac ggcatgaagg
    13441 gcgggctgac gcggctgccg gtgaaggggc gcgatgtctt gatctacagc aatgcggaca
    13501 cggcgggagg cgcgcgcgaa aagctgacgc tgtgggccag ttttgatggc ggccagacgt
    13561 ggccggtgaa gcgcctgctg aatcccggcc acagcgccta ctccagcctg ggggcggcgc
    13621 gtcccggcac agcggacgag gggacaatct acctgctctt tgaaggcggc gagggcgggc
    13681 gctacacggc gatgcagttc gcgcggctga acctgtcgtg gatcgtgcaa ggcggcgagg
    13741 ccaccggcga tggcacggtg ccggactggg cgcggtgact tgtctcacgg caatgcgccg
    13801 ccatttttcc attttcgagt ttgccgtccg cggcggtttc aggctaagaa cccgcatgtc
    13861 ctttcccctg cgctcccctc gcgacactgt cggcggcatc atggtctttg gccggattct
    13921 tgacaagatt cgccttcatg cgcgcgggga gctgccgact ggctggcatc tgggcatcat
    13981 cgcggggaag cgcacttttg acgaccgtgt gtgccgtctg ctgggcgttg acttcgacgc
    14041 gctcagccgc cgcgtgctgg cgggaggaac ggacgatgag gtgctggagt ggtgcttcaa
    14101 aaacggacgc cgccccgatg acgagcacat cgagcttttc aacggcttca tgatgaagcg
    14161 cggctggcgg gacacggcca gcggcggcct ggagcagcaa aagcaggagg ccgggcttgg
    14221 gcaccgcgat gatattgtga cgtttttcga tctcatggac gcggaggagg gccgcgctcc
    14281 gtgattgagg cgggatggca tcatttttga ctcctgaact ctcatggagc cttatctagt
    14341 gatcggactg ctggcgtgcg cgctggttct cttcgccacg gaaaaagttt ccgtggatgt
    14401 ggtgacgctg agcctgctct ccgtgctggt gctcactggc gtgctcagcg cggaggaggc
    14461 cttcgccggg ttcgggcgcg aggtcatcgt catgctcggc agcatttttg tgattggcgc
    14521 tgcgctgcgc gagacgggcg tgctcgacac gctcgggcac ctgctctcgc gcgccgcggg
    14581 cggcggcacc aggcgcctga ccgccggtct gatgtgcggt gtggggggca tctccgcctt
    14641 catgaacaac accaccgtgg tggccatgtt cctcggtcct gtgatcggcg tggcgcgccg
    14701 catgaaggtg ccgccctcgc gcctgctgat gcccctggcc tacgcgtcca tcctgggcgg
    14761 cacctgcacc ctgatcggca catccacgaa tgtggcggtt tccggcgcgc tcaagcaggc
    14821 cggtcatggc gagctgggca tgttcgacat gacacccgtc ggtgtcatga tctgcgcggc
    14881 gggcctgctt tacatgatgc tcgccggcat ccacctcatc ccggagcgcg acaagtccgc
    14941 ccaggccgaa ggcgccgcca atctgcgcga gtatctctcc gaggtcgtga ttctgcaggg
    15001 ttcacccctc atcgggcagg agatattcag ctccgatttc tccgtgcttg gcttccaggt
    15061 catccacatc ctgcggcatg gtctggaact ggacctgggg ccgcactcgc ggttcgagga
    15121 gggggatgtg gtgcgtgtcg cgggcaaggt ggaaaacctc atccgcatca aaaaaatcga
    15181 gggactggac atcatggagg atgtcagcct gcgggagtcc ggcgtggaca tgcgcggcgc
    15241 cagcctggcc gaggtggtgc tgacaccgcg atcctcgctg gtggggcgca ccctgcgcac
    15301 gagcaatttc cgccgccgat ccggcctctc cgttctggcc ctgctgcgcg gggaccagag
    15361 cttgaaggac aaaatggcgg acgtggtgct ggaggcgggc gaccttctgc tggtgcaggg
    15421 gccgtatgac cggctgaaaa cgtatgagga aaactcggag atggtcgtca tcaccgagca
    15481 cgcctgggag atttcggcgc gcaagcgcgg ctacgccctg ctgggcgtct tcggtctcgt
    15541 cgtgctggcc agcagcgccg gctgggtgcc cgccgcggcg ggctttctaa tcgccgcctt
    15601 gttcgccctt ttggcgcgct gcatcacgct ggagacggcc tatgagaaca tagactggag
    15661 gctgctcatc ctcatcggcg gcatgaccgc cttcggcacg gcgatgacga actccggcgc
    15721 tgccgagatg ctggccgggt tcatcaccgg agggttgcaa agctgggggc cgcacgccgt
    15781 cctcgccggc ttctgcgtgc tcaccgtctt gctgacccag ggcctctcca acgccgcggc
    15841 ggcgctcgtc gtgctgccca tcgcgctgcg ctcggcggag tcgcttgcgg tcagtcccgt
    15901 cagctttgga gtggccgtga tgctcagcgc cagcatctcc gtgctcacac ctttcgagcc
    15961 gagctgcatc ctcgtctatg gacctggcaa ataccgtttt gcggacttcg tgaaggtcgg
    16021 cagcgggctg gtgctctgct cgctggccat catcatcgtc atggtgcctg tgctctggcc
    16081 cttttgagtt ccgccgtcag ccgcgccgct tgagcaggat gcgcttgaag cggtcgctgc
    16141 cgctttccac gctcacgctc atcacgtcag ggtcgtcggc gaaggcgttg tggaccatgc
    16201 gccggtagta gctgttcatc ggatcgagca tctgcgactg gccggtgctc cggacccgct
    16261 cgcccatctc gcgcgcgtgc tccacgattt tgtcctcctt catcgagcgg aagtagccga
    16321 tgtccacgcg aatgcgcggc gcgtcgggaa gatgcttttg caggatgcgg ttcaccagat
    16381 actgaatgtc ctccatcgtc gcgccgtggc gtccgatgag cagcttggag tcctccgtat
    16441 gcacctgcag agtggggcct tccgggcctt ccacctcgtc caccttggcg atgaaaccga
    16501 ggtatcccag catggagtcg agaatctggc gggcatgagt gagagcggtc atgcctcctg
    16561 ctatacggga aaacccgccc cctctcaacg gggaagtcgg accatagcca ggccggagca
    16621 gccggcggcg acattctccc gcctgattca gggcagccgc gcctccatca gcgaggtgcg
    16681 gcagaggccc gcgtacagtc cgtccttctc caccagctcc tcatgcgtgc cctgctccac
    16741 gatgcgcccc tgatccagaa cgtaaatccg gtgcgcgtgc cgcaccgtgc tcaggcggtg
    16801 cgcgatgaca aagctcgtgc ggttctccat cagcttctcc agcgccgcct ggatctgcct
    16861 ctccgtctcc gtgtccacac tcgccgtcgc ctcgtccagc agcaggatgg gcgggttccg
    16921 cagcagcgcc cgggcgatcg agacacgctg cttttccccc acgctgagct tgatgccgcg
    16981 ctcgcccacg cggctgtcca gcccgtgctc caggcggcgg acaaaggcgt ccgcgttcgc
    17041 gctcgccagc gcctgccaga gctcctcgtc cgtggcatcg cgcttgccga tgagcagatt
    17101 ctcccgcagc gtgccattga acagaaagct ctcctgcgtc acatacccga tgctccgccg
    17161 caggcttgcc ttgttcacct catgcaccgg cgtgccgtct atcgtcacca cgccctcgtc
    17221 gtattcataa aagcgcgtca gcaggttgat cagcgtggac ttgcccgccc ccgtcggccc
    17281 caccagagcc accgtttctc ccggcttcgc ctccagcgtg atgccatgca ccgtggggat
    17341 actccccccg tagctgaaac ccacgttttc atagcgcaca tggcccgtca ccggccccaa
    17401 ctcgcgcccg cccgccgtgt cactctccgg ctcctcatcc aggatgccga agacacgctc
    17461 ccccgccgcg cgcccgcttt gcaggatctg gttgagctgg tgcagttgct ccaccggatc
    17521 atagaaaaac ttcagcagca gcaggaaagc cgccaggtcc cccttctcga tgcggccctc
    17581 catcagcccg tgcgcgccct gccagagcac gatcaccatg cccgcgctgg tcaggaaatg
    17641 catgcccggc cggtacaccg cccacacccg catcacatgc agtgtcgcct tgcgcagcgc
    17701 gccgctcgcc tcgttaaagc ggtccttctc ctcgcgctcc atcgcgtaag ccttgatctg
    17761 gcgcatcccc gccacgttgt catgcagcag cgagttcatc gcgctgctg
//
LOCUS       DAOMYJ010000090        17769 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Prosthecobacter sp. isolate AMR_MDS_5629
            NODE_1098_length_17769_cov_23.675737, whole genome shotgun
            sequence.
ACCESSION   DAOMYJ010000090 DAOMYJ010000000
VERSION     DAOMYJ010000090.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867850
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prosthecobacter sp. (wastewater metagenome)
  ORGANISM  Prosthecobacter sp.
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Verrucomicrobiaceae; Prosthecobacter.
REFERENCE   1  (bases 1 to 17769)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17769)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.30
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:34:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,742
            CDSs (total)                      :: 4,684
            Genes (coding)                    :: 4,676
            CDSs (with protein)               :: 4,676
            Genes (RNA)                       :: 58
            tRNAs                             :: 55
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17769
                     /organism="Prosthecobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1098_length_17769_cov_23.675737"
                     /isolate="AMR_MDS_5629"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1965333"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2197)
                     /locus_tag="PLP58_11055"
     CDS             complement(<1..2197)
                     /locus_tag="PLP58_11055"
                     /inference="COORDINATES: protein motif:HMM:NF023802.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C39 family peptidase"
                     /protein_id="HRJ09101.1"
                     /translation="MPGTPDEKTIAEIKRLRLAGRALDAWALVSEMDPPEQWPARETR
                     VQGARLLDTLGASKRAERLFWREWRRSKGASDLLQDVFWTVLERRGAYLTWKWLLRHA
                     PGASAPADDLSDYEGCLAVALTRLRDFDAAEAALQRGEALDQKERWFQVLRADLLQEQ
                     DCREAALDAARAAVKQYPDYATAVRTLAELLVELERVEEALEVLRAGVARLQSKGLAA
                     QLAGLLIERGRMDEAEAALDLHERLAPLMDEELRDWHCGRRCDIASARGDHAGALRQA
                     GQVRQGFYQLVAEKLRAAAPGLRRVLLPVPFVRQHHKTCAPASLTSIARFWGREVGHL
                     DLAEEICHDGTPDHREREWAETHGWSAREFAVTVEAAERLIDAGMPFVMDTVHPGGAH
                     AQVVTGYDQFRTVLFIRDPGNPHTTEFLAAEALARQAPFGPRGFVMVPETESERLAAI
                     ELPDTEPHDLLHQTRLRLAEHDRAGARQAVELLRARHPRHRLRWQAELAAAEYDSDHQ
                     ATLEGLEELLRLFPEVENWQTRRLDLIRELRGREACLEALRVVCAKPDSHPIHWRMLA
                     RELHWEAPGIPEARRWLRRVHRARLDSVAILTEANLLWETRDFDNAAPIYRLAACMEE
                     QNEGIWMTWFRAARWTRQPEAALDMLRRRFKQQGATSAAPAVTLYQALDQMNLSHEAL
                     AVLEESLRRRPDDADHALYVAGEMLLWNRCARAREILDSISRGARAAADA"
     gene            complement(2246..4546)
                     /locus_tag="PLP58_11060"
     CDS             complement(2246..4546)
                     /locus_tag="PLP58_11060"
                     /inference="COORDINATES: protein motif:HMM:NF018743.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1343 domain-containing protein"
                     /protein_id="HRJ09102.1"
                     /translation="MRFFLFPALIMFPPPMMAQSFKAEGLRELEALLDKAVAARNPAG
                     AVLRLEHGGSSHTLVKGARALVPAREEMTPDTVFDAASLTKVVATLPCILLLAESGAL
                     HLDDPVCRHLPEFTGDGRERVRLRHLLTHTSGMKPGIPKEPAWTGYAEGVRLACESLP
                     DAPPDTVFRYSDINFILLGEVARRVSGRPLDVLARERVFEPLKMTSTRFAPPEEWRAR
                     IAPTEKDETGVMLRGMVHDPTARRMGGVAGHAGLFTCAEDLARYARMILGGGELEGAR
                     ILSGATLEQARRVQTAGTIAERRGYGWDLESAYSRPRGEVFPPGSFGHTGFTGTSLWL
                     DPHSGGFVIFLSSRLHPAGNGSVRDLYEQVGTAAARCLAGVDFTNVKAPFKARAPGEV
                     PTVLNGIDVLQREGFARLSGMRVGLVTNHTGINARRESTIDLLAAAPGVKLRALFSPE
                     HGIRGQLDQEKIGDSVDKKTGLPVHSLYGERRAPSAEQLGELDALVFDIQDIGCRFYT
                     YISTLRLCMEAAARQGVKFIVLDRVNPVGGAVVEGPALVSKEAFTATHPIAIRHGMTM
                     GELARMMNAERMTGADLEVVPVEGWRREMLFDETGLPWVNPSPNMRTLEAALLYPGIG
                     LLEFSISVGRGTDTPFEILGAPYVDDLRLAHELNKLGLPGLRFMPARFTPSASIFKDQ
                     PCGGARIEITDRRALRPVRAGLAVAAVLQRLYPGKFAVDKVGTLLNHDTLLKALKSGR
                     DARALEDMWRDETADFDGRRRGFLAP"
     gene            4897..9204
                     /locus_tag="PLP58_11065"
     CDS             4897..9204
                     /locus_tag="PLP58_11065"
                     /inference="COORDINATES: protein motif:HMM:TIGR03696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat-associated core domain-containing
                     protein"
                     /protein_id="HRJ09103.1"
                     /translation="MYQATAALAFFPEPDPLVIEVGKTGVVKVKGLPASADVVLEQVL
                     IADPSVASSDFTGTPLTGKGFVNVTFTGLKGGETLVSFIGTNNGDPVFGTTIVKVIQS
                     PGTSAQTPQVGLAGDPVNTATGEYFGLEAVDLNLGGPMPLSFTRYAASNLAQDALADS
                     PLGANRLHNFASKMLSPDDSFKQVVLPDGRAALFRMTSGKWVLASPLDVPYQLLEAGA
                     EFRFGDPHTKQIWTYDNNGRLTKIEDGKGNTHTLNYIAGKLDTVSDGLGRVIEISQPG
                     SLIESVKGMTGAEEREVTYSYTSGVLTSVTDHGGHITTYGNAAGRPTSVTRPEGNELF
                     TQIYTDGKVTSQTERGTDTSTLDYNGAQTTFTDPTGETLVDTYNAEGRLISHADEAGK
                     AITMTYDSAGRRTSVTDRLGRTTTTLYHAPSGLPAAITNAEGRKTLFAYKARTLSGIV
                     FHDLVKITYPDGASRSFVYDAKGNVTQVKDETGKTWKYTYNTRGQVLTAVNPLGGVTT
                     SEYYANGNLSQVQPPDEDATQFTYDPRFRLLEVIHPGGDKIVYAYDTKDRITSVTDER
                     GKVYAFGHDDNNRTTGLTDPDGEQTAVAFDVLDRVQSITDRLGQTASLTYDSRQLVSS
                     VTDQNGNMVSFAYDERQRLTAFNDAAGQAWTFQYDDEDRLIGISNPVDLPASLRRNLM
                     GFAMEASDPLGNTLRVLRDPMQRVTQAFDPLGRQTTRAYDKMGRLVSVTEQGTGTAKY
                     TRDALGNVTKITDPNGGSWSFAYLKSGRLSKITDPLGRNWSYTYDTRGRPSVLNLPDG
                     TLCNYSYDEAGNLTGQQFSSNNPKLDFDYDDLGRLSSTNGVTLQYDKEGRLTQCPQNG
                     KAFSATYDNGGRLATVSYHDSALTVSYYYDSRNRLTKVADSNDITLDMAYDNAGRLTS
                     LTRTPGIDSTFTYDGAGRLTGIQEGGIINLDYTLNAASEVTEVDFTAPVTPSVTPGSQ
                     LLKFGKAGEIVTPGHTYDTRGRLTSSPGRTYGWDGASRLINANGAALTYNGFGDVVTR
                     VESGQTTRFYHHYALGMAPIVYEDTPSGSDRAYVWTPDGHLLYSVDIATGDPIFYHFD
                     RMGSTLALTDKNETVTDSYAYGPFGEPLAHESPGTPSTQPFTYIGKYGVRKEGALYQM
                     RARYYDPQTARFISRDPLPPRLADPKSLNPYEYSGQNPMKFLDPMGEESDSDLGALIL
                     MLCGEIDFNAGDGKLLERKSVKTKKGMTKKGKGKTRKPTRRDVLESAQQAIESWKRRA
                     AASPPPAAGASVSGPPGGGHSGAGTFVCEGPLPKGLGGLFPGLGAPSVAVGDFDGSGR
                     PQILVSGNSGAPAQTPDQDAQGGGLWTGTGNITISNNTISSNTSSPNAAQAMVLDFMI
                     NVYVSLQAAGSELLKKFQDSGYKSKEIRDKMSAVNKALENVGKVIESLGGTRPPPPM"
     gene            9225..10448
                     /locus_tag="PLP58_11070"
     CDS             9225..10448
                     /locus_tag="PLP58_11070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ09104.1"
                     /translation="MKTILPLLTALILLPLSARAVKISYAYDAAGRMTAVNYDGASRT
                     AYGYDKNGSLLSRVSTVTPALTPPPHLAATFSGLITNASPGAANTGVITLKLLANGAY
                     SGKLTVGAVTVSFAGSFAADGGTAVIPITGKPPLTSLDITLDVLGAVPRITGTLSGTG
                     FNSEVAMEAVLYNSKTFPLPAGLVGAYTAVFLPTENTAGIPQGDGYATVTVKNTGAVS
                     LAGKLADNSKLTHGSLIHGVNVWQLYIPMHKNAGFLSGRVLFAPDPGYSDFGGFISWV
                     KPLTAGLHSGEFVTGVDIVGARYRPPLKDQRALGLMNSAPNAVFAASGGGLVTDPFTR
                     NVTLDTKNKFVIPVDAGALKLTLMPGTGLFTGSFKDGAATRAVGGVILQTLNSGSGFF
                     PGATESGLVELTPAP"
     gene            10501..11223
                     /locus_tag="PLP58_11075"
     CDS             10501..11223
                     /locus_tag="PLP58_11075"
                     /inference="COORDINATES: protein motif:HMM:NF041646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VC0807 family protein"
                     /protein_id="HRJ09105.1"
                     /translation="MHAAAKDQPHPLFDLLLTVILPSLVLETMSKPERLGPAWALVVA
                     LLMPLGFGVWCFINKRGLNFFSILGLAAVIFTGGLGLLNLSAVWFAVKEAAFPVFLGI
                     AFPLSLAWGRPLVTEMLLNPQVINHPLLERSLDTQEKRAGFQKLLRQSSWALAGTMLF
                     SALANAWLVLWFLNDVQPGSEAYTRAIGRQNWVGFIVIGIPLTAVTACLLFWLLGRVQ
                     KLTGLDRHDLMNAGQTVRRQVG"
     gene            complement(11236..11325)
                     /locus_tag="PLP58_11080"
     tRNA            complement(11236..11325)
                     /locus_tag="PLP58_11080"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(11289..11291),aa:Ser,seq:gga)
     gene            complement(11756..13390)
                     /locus_tag="PLP58_11085"
     CDS             complement(11756..13390)
                     /locus_tag="PLP58_11085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009961426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HRJ09106.1"
                     /translation="MAARWPPSDFIHPNKHTSKHHIMSAKTEGIAPNASRLLWAGFMA
                     ILAAGVGFSIRAGILGQWAEKYGFTMTELGLITGGGLVGFGVIIMLSSLIADAIGYGK
                     LMFIAFLTHLVSAVLTLSAGYAFEAGGKEAAFQCLYWGMFLFAVGNGVCEAVVNPLVA
                     TLYPKNKAHYLNILHAGWPGGLIVGGLASYAMNGAFTDGKAVTWEIQMALFLVPVVLY
                     GLMIMGQRMPGSEASEAGVGYGVMVMQLFRPLMIVLLVLQALVGYVELGTDSWIGKIT
                     GAIMADPQKGLLLFVYTSGLMFALRFFAGPIVERISPLGLLFVSAVLGFVGLQMLGNA
                     TSVVLCVVAATIYGCGKTFLWPTMLAVVSERFPKGGAITIGAVGGVGMLSAGLLGGPG
                     IGFKQDFHASAQLKDSHPAIYERYKAGNENSFVGYKTVGLDGAKVGVLDDSGKELARA
                     GEILAKENKTDANHATLAKWWEDASKSAAEDKAPVTAAGLHGGRMALKLTSYVPLAMA
                     LIYLLLILYFKVTGGYRAIHVTGEQMTGGTQGPMEA"
     gene            complement(13411..15021)
                     /locus_tag="PLP58_11090"
     CDS             complement(13411..15021)
                     /locus_tag="PLP58_11090"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HRJ09107.1"
                     /translation="MSNDGKPSMALFWGCFIALITTSYAFFSRMYLCDVRFGLDFGID
                     KVTIGALKGAGIWPFAISIILFSLIIDRIGYKIAMLFSFVCYLIYAGMAFMAYGAIQG
                     VTGDALVAAQARGYNLLYWGSVILGLGNGTVEAFINPVVATMFSKDKTKWLNILHAGW
                     PGGLVFGGLCTIALAGVAASGDWRIVLGLVLVPAVIYLVMLIGAKFPVNEREQAGVSY
                     VQMLGEFGVFGALVAFGLIFAQLEQVPELSSVFFAPYAKWIYTGVVAALFGLVTKSFG
                     RPLLAFMIVIMGPLATTEIGTDGWINSLMEEPMKAAGANPGWVLIYTSAIMMVLRFFA
                     GPIVHKLSPIGLLITSSVLAIAGLLALSKTGSSGLLIIFAAATLYGFGKTFFWPTMLG
                     ITAEQSPKGGALTLNAISGIGMLAVGILGFPFIGSLQEDTTTKLIQEKNSALVEQITV
                     DKKYVLGAYKGVDPVKAAAVTAEEDKSMIAEATKAGQFTALGRMAMFPAFMLVCYMLM
                     FLYFKSKGGYKAVDLMAPDKEGGQMEQA"
     gene            15233..16660
                     /locus_tag="PLP58_11095"
     CDS             15233..16660
                     /locus_tag="PLP58_11095"
                     /inference="COORDINATES: protein motif:HMM:NF018178.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1080 domain-containing protein"
                     /protein_id="HRJ09108.1"
                     /translation="MKKASFLLPLLAAALAPAVFAEDNVPTAGFTALFNGRDLSGWYG
                     WGTQDPADLWNMSEEERAAYKKKSIEGGLLDAKGNDKGDHVNAHWKVENGELVNDGKG
                     LYLTTDKDYGDFELWVDYKMLPKGDSGIYLRGTPQVQIWDFTETDEKAVSLGKPKGSG
                     GLWNNKNPEGKDPLKLMDKPLGEWNRFYIRMIGERVLIKLNGEVVVNNAVLENFFANR
                     KAGYLAYGKKDDKAKAAEKPPAGWMQDPVYAKGPIQLQTHGSEIRWKNVFVREIPADE
                     ANQILAQGEEGFTRLDNGQDLSNWQGAVDNYEVVEGSIVCKQGKGGDLLSKEEYGDMV
                     LRFEFKLPPAGNNGIALRTPVGGHSAKDGLEIQVIDTDGYEAKTGKELQPYQVHGSVY
                     HLLGAKRGYLRPAGEWNYQEVEVRGNQIKVTLNGTRILDANLDELDYSGVKTKPAGVE
                     RKTGHIGFAGHSDPVAFRNFRVKRL"
     gene            16810..>17769
                     /locus_tag="PLP58_11100"
     CDS             16810..>17769
                     /locus_tag="PLP58_11100"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HRJ09109.1"
                     /translation="MSDEIYPQDSSPDSPPPRTTGGNWRWEPPSPEELQKLMPGYTIE
                     KLLGRGGMGAVYRGIQMNLDRPVAIKILPPGVEKEDSSFAERFKNEAKLMAKLNHPAV
                     VSVHDFGATQGGQLYFAMEYVDGSDVSQMIAAQGKLPPDHALAITAHVCDALAAAHEL
                     GIVHRDIKPANVLINMKGQVKVADFGLAKVEEPGQHGFTKTGYAMGTPDFVAPEALML
                     GMAIDGRADIYAVGVMLYQMLTGNIPRGAFKSAAQLVPGLDPRFDAIITKAMQSARED
                     RHQSAAELRRELDVILTVPLVRQDAPVSAAIPVAQVAQAPGQRS"
BASE COUNT         3529 a         5829 c         5320 g         3091 t
ORIGIN      
        1 cagcatcagc cgcggcgcgc gcgccgcggc tgatgctgtc gaggatttct ctcgcgcgcg
       61 cgcagcggtt ccacaacagc atctctcccg ccacatagag ggcgtggtcc gcgtcatccg
      121 gccttctgcg gagagactcc tccagcacgg ccagggcctc gtgggagaga ttcatctgat
      181 ccagtgcctg ataaagcgtg acggcgggtg cggcggaggt ggcgccctgc tgcttgaaac
      241 ggcggcgcag catgtccagc gcggcctccg gctggcgggt ccagcgcgcg gcgcggaacc
      301 atgtcatcca gatgccttca ttttgctcct ccatgcacgc tgccaggcgg tagatcggcg
      361 cggcattgtc aaagtcccgc gtctcccaga gcaggttcgc ctcggtgagg atggccacgg
      421 aatcgagccg ggcgcgatgc acgcggcgca gccagcgcct ggcctccggg atgcctggcg
      481 cctcccagtg cagctcgcgc gcgagcatgc gccagtggat ggggtggctg tccggcttgg
      541 cgcacaccac gcgcaatgct tcgagacagg cctcccgccc gcgcagttca cggatgaggt
      601 cgagccggcg tgtctgccag ttttccactt ccgggaagag cctcagcagt tcctcaagcc
      661 cctccagagt ggcctggtgg tctgaatcat actcggccgc cgccagttcc gcctgccagc
      721 gcaggcggtg ccgcggatga cgcgcacgca gaagctccac ggcctggcgc gctcccgcgc
      781 gatcatgctc cgccagccgc agccgggtct ggtgcagcaa gtcatgcggc tcggtgtccg
      841 gcagttcgat ggcggccagc cgctccgact ccgtctccgg caccatgacg aatccgcgcg
      901 ggccgaaggg cgcctgccgc gcgagcgcct cggcggccag aaactcggtg gtgtggggat
      961 tgccggggtc gcgaataaaa agcacggtgc ggaattgatc gtaaccggtc accacctgcg
     1021 cgtgcgcgcc tccgggatgg accgtgtcca tgacaaaagg catgcccgcg tcaatgagcc
     1081 gctccgcagc ctccacggtg acggcgaatt cccgcgccga ccagccgtga gtctcggccc
     1141 attcccgctc gcgatggtcc ggagtgccgt cgtggcagat ttcctccgcc aggtccaggt
     1201 gccccacttc cctcccccag aagcgggcga tgctggtcag ggaagcaggc gcgcaggttt
     1261 tgtggtgctg gcgcacaaaa ggcacgggca gcagcacgcg gcgaaggccg ggagcggcgg
     1321 cgcgtaattt ttccgccacc agttgataaa aaccctgccg cacttgcccg gcctggcgca
     1381 gagccccggc atggtctccg cgcgcgctgg cgatgtcgca ccgccgcccg caatgccagt
     1441 cccgcagttc ctcatccatg aggggagcca gacgctcgtg caggtccagc gcggcctctg
     1501 cctcatccat ccgtccgcgt tcgatcaaaa gtcccgccag ttgcgcggcc agccctttgc
     1561 tttgcagacg cgccaccccc gcgcgcaaga cctccagcgc ctcttccacg cgctccagct
     1621 ccaccagcag ttctgcgagg gtccgcaccg ccgtggcata gtccgggtac tgcttcaccg
     1681 ccgcgcgagc cgcatccaat gccgcctccc tgcagtcttg ttcctggagg aggtcagcgc
     1741 gcaggacctg gaaccagcgc tccttttggt ccagtgcctc gcccctctgc aaagctgcct
     1801 ctgccgcgtc gaagtcccgc agccgtgtca acgccacagc caggcagccc tcgtagtcag
     1861 acaaatcatc ggcaggcgct gacgcgccgg gggcgtgccg cagcagccac ttccaggtca
     1921 ggtaggcgcc gcgccgctcc agcacggtcc aaaacacatc ctgcagcaga tcggacgcgc
     1981 ctttcgagcg ccgccactcg cgccaaaaga ggcgctccgc gcgcttggag gcgcccagtg
     2041 tgtccaggag gcgcgcgccc tgcacgcgcg tctcgcgtgc gggccattgt tccggcgggt
     2101 ccatttctga gacgagcgcc caggcatcga gcgcgcgccc ggcgagccgg agccttttga
     2161 tttcagcgat ggttttttcg tcaggtgttc caggcatggg ggtgtatgga ttgatgtgtg
     2221 ccgcctgcag caggccgtga gtctctcaag gcgctaaaaa gccccgccgc ctgccgtcaa
     2281 agtcggccgt ctcatcccgc cacatgtcct caagggcgcg cgcgtccctg ccggacttga
     2341 gcgccttcaa gagcgtgtcg tggttgagca gcgtgccgac cttgtccacg gcgaattttc
     2401 ccggataaag ccgctgcagg acggctgcga cggccagccc ggcgcgcacg gggcgcaggg
     2461 cgcggcggtc ggtgatctca atgcgcgcgc cgccgcaggg ctggtccttg aaaatgctgg
     2521 ccgagggcgt gaagcgcgcg ggcatgaagc gcagccccgg caggccgagc ttgttcaact
     2581 catgggccag gcggaggtcg tccacatagg gcgcgccgag gatttcaaac ggcgtgtccg
     2641 tgccgcgccc gacgctgatg gaaaactcca gcaggccgat gccgggatag agcagcgcgg
     2701 cctccagcgt gcgcatgttc ggggaggggt tgacccacgg caggccggtc tcatcgaaga
     2761 gcatctcccg ccgccagccc tcaaccggca ccacctccag gtccgcgcct gtcatgcgct
     2821 cggcattcat catccgcgcc agctcgccca tggtcatgcc gtggcggatg gcgatgggat
     2881 gcgtggcggt gaaggcctcc tttgacacga gggcggggcc ttccaccacg gcgccgccga
     2941 cggggttcac gcggtcgagc acgatgaatt tcacgccctg cctcgccgcc gcctccatgc
     3001 acaggcgcag cgtggagatg taggtgtaga agcggcagcc gatgtcctgg atgtcaaaga
     3061 ccagcgcgtc gagttccccg agctgctccg ccgacggcgc gcgcctttca ccgtaaaggc
     3121 tgtgaacggg caggccggtc ttcttgtcca cgctgtcgcc gatcttttcc tggtcgagct
     3181 ggccgcgaat gccatgctca gggctgaaca gggcgcgcag tttgacgccc ggcgcggcgg
     3241 ccagcaggtc aatcgtgctc tcccgccgcg cgttgatgcc ggtgtggttg gtgacgagtc
     3301 ccacgcgcat gcccgacaag cgcgcgaagc cctcgcgctg gagcacgtct atgccgttga
     3361 gcaccgtcgg cacctcgccg ggcgcgcggg ctttgaaggg cgccttgacg tttgtaaaat
     3421 ccacccccgc caggcagcgc gccgccgccg tgcccacctg ctcgtaaaga tcgcgcacgc
     3481 tgccgttgcc cgccggatgc agtcgcgagc tgagaaaaat gacaaagccg cccgagtgcg
     3541 ggtccagcca caagctcgtg ccggtgaagc ccgtgtggcc gaagctgccg ggcgggaaga
     3601 cctccccgcg cgggcggctg taagcgctct ccaggtccca gccgtatccg cgtctttccg
     3661 cgatggtgcc cgccgtctgc acgcggcgcg cctgctccag tgtggcgccg ctgagaatgc
     3721 gcgcaccctc cagctccccc ccgcccagga tcatgcgcgc gtagcgggcc aggtcctccg
     3781 cgcaggtgaa aaggcccgca tgcccggcca cgccgcccat gcgccgcgcg gtggggtcat
     3841 gcaccatgcc gcgcagcatg acgccggtct catccttctc cgtgggcgcg atgcgcgcgc
     3901 gccactcctc aggcggggca aagcgggtgg atgtcatttt gagcggctcg aagacacgct
     3961 cccgcgccag cacatcaagc ggacggcctg aaacccgccg cgccacctcg cccagcagga
     4021 tgaaattgat gtcgctgtag cggaaaaccg tgtccggcgg cgcgtcgggc aggctttcac
     4081 aggccaggcg cacgccctcg gcgtaacccg tccatgcggg ttccttcgga atgccgggtt
     4141 tcatgccgga ggtgtgcgtg agcagatggc gcagccgcac ccgttcgcgc ccgtcaccgg
     4201 tgaactccgg tagatgccgg cacactgggt cgtccaggtg cagtgcgccg ctctccgcca
     4261 gcagcaggat gcatggcagt gtggcgacga ccttggtgag cgaggcggca tcgaagactg
     4321 tgtcaggcgt catctcctcg cgcgcgggga ctagggcgcg cgcgcctttg accagggtgt
     4381 ggctggaacc gccgtgctcc agccggagca cggcaccagc ggggtttctc gctgccacgg
     4441 ctttgtcgag caaggcctcc agctcgcgca ggccttcggc tttgaacgac tgcgccatca
     4501 tcggcggcgg aaacatgatg agggcgggaa aaagaaagaa gcgcatgatc acagacaacc
     4561 agaccggcgt tgcggagtca atcctgggcc cggaaaggcg cgggttcttt ccattctgct
     4621 cgtcagccag ccaggcagac atcctgactg ggagatggat ccagaaataa aaagctcgct
     4681 ttggtgaggc gggatgggta ttttttcaat tatgaaaacc aggccctggt tgcactcaaa
     4741 tgcgtgctgt cccgacgcgt ttcgtgatgc tgttgcagaa gcctgggatc gccagacgcc
     4801 ggcatgaccg gcgctcacaa cccctgatcc gcacaagcca tgcaccctca ttttatcctc
     4861 ccgccgcttc gccgcttgct cttggccgcc gttgtcatgt accaggcaac cgccgcgctg
     4921 gccttttttc ctgaaccgga tccgctggtg attgaggttg gcaaaacggg cgtggtgaag
     4981 gtgaaggggc tgcctgcttc agcggatgtg gttttggagc aggtgctgat cgccgaccct
     5041 tcagtcgcca gttcggactt cacaggtaca ccactcaccg gcaaaggctt tgtgaatgtg
     5101 actttcactg gactgaaagg cggcgaaacg ctggtttcat tcattggcac gaacaatggc
     5161 gatccggtct tcggaaccac catcgtgaaa gtcatccaat cgccaggcac ctccgcccag
     5221 accccgcagg tgggtctggc gggcgaccct gtgaacacgg ccactgggga gtacttcggc
     5281 ctggaggccg tggacttgaa tctcggcggg ccgatgccgc tgtccttcac ccgttacgca
     5341 gcctccaatc tggcccagga cgctctcgcg gacagccccc tgggcgcgaa ccggctgcac
     5401 aacttcgcct cgaagatgct gtctccagac gattcattca aacaggtggt gcttcccgac
     5461 ggacgggcgg ccctgttccg aatgacctct ggaaaatggg ttctcgccag tccgctggat
     5521 gtgccctacc agctgctgga ggcgggcgct gagttccggt tcggcgatcc tcataccaag
     5581 caaatctgga cctacgacaa caatggccgc ctcaccaaga tcgaagacgg aaaaggcaac
     5641 acacatactt taaattacat cgccggaaag ctggacactg tcagcgatgg acttggccgc
     5701 gtcatcgaga tcagccagcc gggcagcctc atagaaagcg tgaagggaat gactggagcc
     5761 gaagaaagag aagtcactta cagctacaca agcggcgtgc tcacctctgt cactgaccat
     5821 gggggacaca tcaccactta cggcaatgcg gctggtcgtc ccacctccgt cacgcgcccc
     5881 gagggcaacg agcttttcac ccagatctac accgatggca aagtgaccag ccagactgaa
     5941 cgcggcacgg acaccagcac cctggattac aatggagcgc aaaccacctt caccgaccca
     6001 acgggcgaaa cgctcgtgga cacctacaac gctgagggcc gcctgatcag ccatgctgac
     6061 gaggccggca aggccatcac catgacgtat gacagcgccg gccgacgcac cagcgtcacg
     6121 gaccggctgg gccgcaccac gaccacactt taccacgccc ccagcggcct gccggcggcg
     6181 atcaccaacg cggaggggag gaaaaccctc ttcgcctaca aggcgcgcac tctgtccggc
     6241 atcgtgtttc acgatctggt caagatcacc taccccgacg gagcctcgcg cagctttgtc
     6301 tatgacgcca aaggcaacgt gacacaggtc aaggacgaga caggcaagac ctggaaatac
     6361 acctacaaca cgcgcggcca ggtgctcacg gcggtgaatc ctctcggcgg cgtgaccacc
     6421 agcgagtatt atgccaacgg caatctcagc caagtccagc cgcccgacga ggacgccacg
     6481 cagttcactt atgacccgcg cttccgcctc ttggaggtca tccaccccgg aggtgacaag
     6541 atcgtttatg cctacgacac caaggaccgg atcacctccg tgaccgatga gcgtggcaaa
     6601 gtttacgcgt ttggccatga cgacaacaac cgcaccaccg gcctgacgga tcccgatggc
     6661 gagcagaccg ccgtggcctt cgatgtgctg gatcgcgtcc agtccatcac cgaccgcctc
     6721 ggacagaccg cgtccctcac atacgattcg cggcagttgg tgtcctccgt caccgatcaa
     6781 aatggcaaca tggtcagctt tgcttacgac gagcgccagc gtctcaccgc tttcaatgac
     6841 gcggcagggc aggcctggac cttccaatac gacgacgagg accgcctcat cggcatcagc
     6901 aaccccgtgg accttccggc cagcctgcgg cggaacctca tggggtttgc catggaggcg
     6961 agcgatcctc tgggcaacac cctgcgcgtg ctgcgtgacc ccatgcaacg ggtcactcag
     7021 gccttcgatc ctctcggtcg ccagacgact cgtgcttatg acaagatggg caggctggtc
     7081 agcgtgaccg agcagggcac cggcaccgcc aagtacaccc gcgatgcgct tggcaatgtg
     7141 accaagatca ccgaccccaa cggcggctcc tggagctttg cttatctgaa gtccggcagg
     7201 ctctccaaaa tcaccgaccc gctcggcagg aactggagct acacttacga cacccgcggc
     7261 cgcccttccg tgctcaacct cccggatggc acactttgca actacagcta cgacgaagca
     7321 ggcaacctca ccggccagca gttcagcagc aacaatccca aactcgattt tgattatgac
     7381 gatctcggca ggctctcctc caccaacggg gtgacactcc agtatgacaa agaagggcgg
     7441 ctgacccaat gcccgcaaaa cggcaaagcc ttctccgcca cctatgacaa cggcgggcgc
     7501 ctggccaccg tgagctacca cgacagcgcg ctcaccgttt cctactacta cgacagtcgc
     7561 aaccggctca caaaggtcgc cgacagcaac gacatcacgc tggacatggc ctatgacaac
     7621 gcgggccgcc tgaccagcct cacccgcact ccgggcattg actccacctt cacctatgac
     7681 ggagcgggca ggctgactgg catccaggag gggggcatca tcaatctgga ctacacgctc
     7741 aatgcggcgt ctgaagtgac ggaggtggat ttcacggctc cagtgacgcc ttccgtgact
     7801 cccggctcgc aactgctgaa atttggcaaa gccggggaaa ttgtcacccc cggccacacc
     7861 tacgacacac gcggcagact cacctcctca cccggcagaa cctatggctg ggatggcgcc
     7921 tcgcgcctga tcaatgccaa tggagccgcc ctcacctaca acggctttgg tgacgtggtc
     7981 acgcgcgtgg aatccgggca gaccacccgc ttttaccatc actacgcgct cggcatggcc
     8041 cccattgtct atgaagacac cccttccggc tccgaccgtg cttatgtctg gacgccggac
     8101 ggtcatcttc tttacagcgt ggacatcgcc acaggagacc ccatttttta tcacttcgac
     8161 cgcatgggca gcaccctggc gctgacggac aaaaatgaaa cggtgactga cagctacgct
     8221 tatggtccat tcggtgagcc gctggcccat gaaagtcctg gtacgcctag cacgcagccc
     8281 ttcacctaca ttggtaaata cggggtgcgc aaagagggcg cgctttacca gatgcgtgcc
     8341 cgctactatg acccgcagac cgcgcgcttc atctccagag acccgctgcc gccgcgcctg
     8401 gcggacccca aatcgctgaa cccgtatgag tattcggggc agaacccgat gaagtttttg
     8461 gacccgatgg gggaagaaag tgacagtgac ttgggagctt taatactgat gctgtgtggt
     8521 gaaattgatt tcaatgcagg tgatggaaag cttctcgaaa ggaaaagtgt caaaacaaag
     8581 aaaggtatga caaagaaagg taaaggtaag acaaggaaac ctacgcgtcg ggatgtgctg
     8641 gaatcggccc agcaagccat cgaatcctgg aaaagacgcg cggccgcctc gccgccacct
     8701 gcagccgggg ccagcgtctc cggccctcct ggcggcgggc acagcggtgc gggaactttc
     8761 gtctgcgagg gaccattgcc caaaggactt gggggattgt ttcccggcct tggagccccg
     8821 tctgtggcgg tgggtgattt cgacggcagc ggaagacccc agatcctggt ttccggcaat
     8881 tcaggcgcgc cagcacagac tcccgatcaa gacgcacagg gcggcggcct atggactggg
     8941 actgggaata tcaccatctc caacaacacc atctccagca acacatccag tccgaacgcc
     9001 gcccaggcga tggttctgga tttcatgatc aacgtctatg tctctctcca ggcagccggc
     9061 agcgagctgc tgaaaaagtt ccaggactcc ggctacaaga gcaaagaaat ccgcgacaag
     9121 atgagcgcgg tgaacaaagc cctcgaaaac gtcggcaagg tcattgagtc cctcggcggc
     9181 acccgcccgc cgcctccgat gtgaaccttc attccttcac tctcatgaaa accattctgc
     9241 cccttctcac cgcgctgatc ctgctgcccc tctcagccag ggctgtgaaa atcagctatg
     9301 cctacgacgc cgccgggcgc atgacggcgg tgaactatga cggcgccagc cgcaccgcct
     9361 acggctatga caaaaacggc agcctgctgt cccgtgtcag caccgtcacg cccgcgctca
     9421 cgccgccgcc gcacctggcc gccaccttca gcggcctcat caccaacgca tcgccggggg
     9481 ccgccaacac cggcgtcatc accctcaagc tcctggccaa cggagcctac agcggcaagc
     9541 tcaccgtggg cgcggtcact gtctcctttg caggcagctt cgccgccgat ggcggcacgg
     9601 cggtcatccc catcaccggc aaaccgccgc taacctcact ggacatcaca ctggatgtgc
     9661 ttggcgccgt gccccgcatc accggcacgc tgagcggcac cggcttcaac tccgaagtcg
     9721 ccatggaggc cgtgctttac aacagcaaga ccttcccgct gcccgccggc ctcgtcggag
     9781 cctacaccgc cgttttcctg cccacggaaa acaccgccgg cattcctcaa ggcgatggtt
     9841 atgccacggt gacagtgaaa aacacaggcg ccgtgtccct ggccggcaaa ctggcggaca
     9901 actccaagct cacccacggc agcctgattc acggcgtcaa tgtctggcag ctttatatcc
     9961 cgatgcacaa aaacgcgggc ttcctctccg ggcgcgtcct cttcgcgcct gaccccggat
    10021 acagcgactt cggcggcttc atctcgtggg tgaaacccct cacagcgggc ctgcactcgg
    10081 gtgagtttgt caccggagtg gatatcgtcg gcgcgcgcta ccggccgccc ttgaaagacc
    10141 agcgcgcgct gggcctcatg aattccgcgc ccaacgccgt cttcgccgcc agcggcggcg
    10201 ggctcgtcac agatccgttc acgcgcaatg tgacactcga cacgaaaaac aagttcgtca
    10261 tccccgtgga tgccggcgcg ctgaagctca ccctgatgcc gggcacgggg cttttcaccg
    10321 gcagcttcaa ggacggcgcc gccacccgcg ccgtcggcgg cgtcatcctg cagaccctca
    10381 acagcggctc cggcttcttc cccggcgcca ccgagagcgg cctcgtcgaa ctcacccccg
    10441 cgccctgacc tggcgatcag cggttgagat ttcgcagcct ggcgccaagg atgcgcgcgc
    10501 atgcacgccg ccgccaaaga ccagccacat ccgctctttg atcttctcct caccgtcatt
    10561 ctaccctccc tggtgctgga gacgatgagc aagccggaac ggctcggccc cgcctgggcg
    10621 cttgtcgtcg cgctgctcat gccgctggga ttcggcgtct ggtgcttcat caacaagcgc
    10681 gggctgaatt ttttctccat cctcggcctt gccgccgtga ttttcaccgg cggactcggc
    10741 ctgttgaacc tgagcgccgt ctggtttgcc gtgaaggagg ccgccttccc ggtctttcta
    10801 ggcatcgcct tccccttaag cctggcctgg ggcaggcctc tcgtcacaga aatgctgctc
    10861 aatccgcagg tcatcaatca tccgcttctg gaacgctccc tggacacaca ggaaaaacgc
    10921 gccggctttc aaaaactcct gcgccaatcc tcctgggcac tggccggcac catgctgttc
    10981 tccgctttgg caaacgcctg gcttgtgctc tggtttttga atgacgtcca gccaggcagc
    11041 gaagcctaca cccgcgccat cggcaggcaa aactgggtcg gcttcatcgt catcggcatc
    11101 cctctcaccg ctgtcacagc ctgcctgttg ttctggcttc ttggccgcgt gcaaaaactc
    11161 accggcctgg acaggcacga tctcatgaac gccggtcaga ccgtgcgccg ccaggtaggc
    11221 tgactggcgg ggacgtggcg gacagagagg gattcgaacc ctcggtgcct tttacagcac
    11281 acacgctttc caggcgtgcc cgatcgacca ctctggcatc tgtcctcaag caggctgcat
    11341 ttcgcagcgg gcgcagactc tgccgcctgg cacggggcgt ggcaagttga ttttccatcc
    11401 gtttgttcgg caaccgctca atggcagccg caacccgtgc cgcatgatcc acccgggggc
    11461 atggagggcc ccgcccctcc cataagtccg gtgccgccaa taataaccct tcgtaccgct
    11521 cggcctgttt cagggtccgc ggtcaaaggc gcgtcattca tgctttgacg cacctcgaac
    11581 cggcgcggca cttcattttc agactcgggg atgacttcgt aaatgtaggt gggcatggaa
    11641 tgttggggtt gttcttcccc ggcacgaatg acgatagccg cgcctgctgg caagaccaaa
    11701 aatgaaaacg gcgtccccgg taagggggga cgccgattgg aggaatcaag cggaatcaag
    11761 cctccatggg tccctgagtg ccgcctgtca tttgctcgcc agtcacatga atggctctgt
    11821 agccgcccgt gactttgaaa taaagaatga gcagcaggta gatcagcgcc atcgccagcg
    11881 gcacatagga tgtgagcttc aacgccatcc tgccgccatg caggccagcg gccgtcaccg
    11941 gagccttgtc ctcagcggct gattttgacg cgtcttccca ccacttggcg agagtggcgt
    12001 ggttggcgtc tgttttgttc tctttcgcca aaatttctcc ggctcgtgcc agttctttgc
    12061 cggagtcgtc caggacaccc actttggcac cgtcaagacc cacggtttta taacccacga
    12121 agctgttttc atttcccgcc ttgtaacgtt cataaatcgc cggatgagaa tctttaagct
    12181 gtgcggatgc atgaaaatct tgcttgaaac cgatgccggg accgccaaga agcccggcag
    12241 acagcatgcc gacgccaccg accgcgccaa tggtgatggc gccacccttg ggaaagcgct
    12301 cggagacgac agcgagcatc gtcggccaga gaaaggtctt gccgcagcca taaatcgtgg
    12361 cggccaccac gcagagtacc accgaggtgg cattgcccag catttgcagc cccacaaatc
    12421 caagaaccgc gctgacaaac agcaagccaa gcggggaaat gcgctccact atcggcccgg
    12481 caaagaatcg cagcgcgaac atcaagcccg aagtgtaaac aaacagcagc aggcctttct
    12541 gcgggtcggc catgatcgcc cctgtgatct tgccgatcca ggagtccgtg cccaactcca
    12601 cataaccgac gagcgcctgc aaaacgagga gcacgatcat gagaggccta aagagctgca
    12661 tcaccatgac tccgtaaccc acgccagcct cgctcgcctc ggagccgggc atccgctgtc
    12721 ccatgatcat caaaccatat agaacgacag ggacgaggaa aagggccatc tggatttccc
    12781 aggtcaccgc cttgccatcc gtgaaagcgc cattcatggc gtatgaggcc agaccaccga
    12841 caatgagtcc gcccggccag cccgcgtgga gaatgttcag atagtgcgct ttgtttttcg
    12901 ggtatagagt ggccaccaac gggttcacaa cggcctcgca aacaccattg cccaccgcga
    12961 agaggaacat gccccagtaa aggcactgga acgcggcctc tttgccaccc gcctcaaagg
    13021 cataaccagc gctgagcgtc agcactgcgg aaacgagatg tgtcagaaaa gcgatgaaca
    13081 tcaacttccc gtaaccaatg gcgtcggcaa tcagcgagct gagcatgata atgacaccaa
    13141 agccgacaag gccaccgccg gtgatcaaac ccagttcggt catggtaaac ccatattttt
    13201 ccgcccattg gccgagaatg ccggcgcgaa tggaaaagcc gacgccggcg gcgaggatgg
    13261 ccatgaaacc ggcccagagg agccggctgg cgttgggtgc gatgccttct gtttttgctg
    13321 acatgatgtg gtgtttggat gtgtgtttgt tgggatgaat aaaatccgat ggcggccacc
    13381 gggccgccat cgggctggat cgaaccttcc ttatgcctgc tccatctggc cgccttcctt
    13441 gtccggggcc atgaggtcca ccgctttata gccgcctttg gacttgaagt agaggaacat
    13501 gagcatgtag cagaccagca tgaaggccgg gaacatggcc attctgccaa gcgcggtgaa
    13561 ctggcccgcc ttggtcgcct cggcaatcat gcttttgtcc tcctctgcgg tgacggcggc
    13621 ggctttgacg gggtccaccc ctttgtaggc gccgagcacg tattttttat ccaccgtgat
    13681 ctgttcgacc agcgcgctgt tcttttcctg gatgagcttg gtggtggtgt cctcctgcag
    13741 ggagccgatg aagggaaagc ccaggatgcc cacagccagc atgccgatgc cggagatggc
    13801 gttcagtgtc aacgcgccgc ctttgggact ctgctcggcg gtgatgccga gcatggtggg
    13861 ccagaagaaa gtcttgccga agccgtaaag cgtcgcagcg gcgaagatga tgagcaggcc
    13921 ggaactgccg gtctttgaca aggccagaag gcccgcgatg gcgaggacag agctggtgat
    13981 gagcaggccg atgggggaga gcttgtggac gatggggccg gcaaagaagc gcagcaccat
    14041 catgatggcg gaggtgtaaa tgagcaccca gcctggattt gcccctgcgg ctttcatggg
    14101 ttcttccatc agggagttga tccagccgtc tgtgccgatc tctgtggtcg ccagcggtcc
    14161 catgatgacg atcatgaagg cgagcaacgg cctgccgaag ctttttgtca ccagcccgaa
    14221 cagggccgcg acaacgccgg tgtatatcca tttggcataa ggggcgaaga aaacgctgga
    14281 aagctccggc acctgctcca actgcgcgaa gatgaggcca aaagccacca gcgcgccgaa
    14341 cacgccgaac tcgccgagca tctgcacata actgacgccg gcctgctcgc gctcgttgac
    14401 cggaaatttc gccccgatga gcatgaccag ataaatcacc gccggtacca gcacgaggcc
    14461 gagcacgatg cgccagtcgc cgctggcggc gacacccgcc agcgcaatgg tgcaaagacc
    14521 gccaaacaca agaccgcccg gccagcccgc gtggaggatg ttcagccact tggttttgtc
    14581 cttgctgaac atggtggcaa cgacagggtt gatgaaggct tccaccgtgc cattgccgag
    14641 tccgaggatc acgctgcccc agtagagcag gttgtacccg cgcgcctggg ccgccacgag
    14701 cgcgtcacct gtcacaccct ggatggctcc ataggccatg aaggccatgc ccgcgtagat
    14761 caagtagcag acaaaggaaa acagcatggc gattttgtag ccgatgcggt caatgatgag
    14821 gctgaacagg atgatggaga tggcgaaggg ccagatgcca gcccctttca gcgcgccgat
    14881 ggtgacttta tcaatgccga aatccaggcc gaaccgaacg tcgcacagat acatgcgcga
    14941 aaagaaggcg taggatgtgg tgatgagggc gatgaagcag ccccagaaca gggccattga
    15001 aggtttgccg tcgttgctca tggttgggtt ggtatgtggg atggatgttg ctttgtcggc
    15061 ccgcgcggac gtaccggccg gcagccaacg ggagaagcgg cgcgttttta tgcgggcagt
    15121 tgaggtgcga caagaaaaaa agcggcgatt ttcacaaatt cgttagtccc ctccgcgccc
    15181 ggcattcatg catgaaagaa tcccgcccgc gtgcccgact ccctttccca ccatgaagaa
    15241 agcatccttt ttgctccccc tgctcgccgc tgcgctcgcg ccggcggttt tcgctgaaga
    15301 caatgtcccc accgccggct tcaccgccct gttcaatggc agggacctct ccggctggta
    15361 tggctggggc acgcaggacc ccgccgacct ttggaacatg agcgaggagg agcgcgccgc
    15421 ttacaagaaa aaatccatcg agggcggcct gctcgacgcc aagggcaacg acaagggcga
    15481 ccatgtgaac gcgcattgga aggtggaaaa cggcgagctg gtcaatgacg gcaagggcct
    15541 ctacctcacc accgacaagg actatggcga cttcgaactc tgggtggact acaaaatgct
    15601 gcccaagggc gacagcggca tctatctgcg cggcacgccg caggtgcaga tctgggactt
    15661 cactgaaaca gacgaaaaag ccgtctccct cggcaagccc aagggctccg gcggcctctg
    15721 gaacaacaag aacccggagg gcaaagaccc gctcaagctc atggacaagc cgctgggcga
    15781 gtggaaccgc ttttacatcc gcatgatcgg cgagcgcgtg ctcatcaagc tcaatggcga
    15841 ggtcgtggtg aacaacgccg tgctggaaaa cttcttcgcc aaccgcaagg ccggctatct
    15901 ggcctatggc aagaaagacg acaaagccaa ggccgccgaa aagccgcccg caggctggat
    15961 gcaggatccg gtttacgcca aaggccccat ccagctccag acccacggca gcgagatccg
    16021 ctggaagaat gtcttcgtgc gtgagattcc ggctgacgag gcaaaccaaa tcctcgccca
    16081 gggtgaggaa ggtttcacac gtctggacaa cggccaggac ctctcgaact ggcagggagc
    16141 cgtggacaat tacgaggtcg tggagggcag catcgtctgc aagcagggca aaggcggcga
    16201 cctgctcagc aaggaggagt atggcgacat ggtgctgcgc tttgaattca agctcccgcc
    16261 cgccggcaac aacggcatcg ccctgcgcac tcccgtgggc ggtcacagcg ccaaggacgg
    16321 tctggagatc caggtcattg acaccgacgg ctacgaggcc aagaccggca aggaactcca
    16381 gccctaccag gtgcatggct ccgtgtatca cctgctcggc gccaagcgcg gctacctgcg
    16441 cccggccggc gagtggaact atcaggaagt cgaagtgcgc ggcaaccaga tcaaagtcac
    16501 cctcaacggc acccgcatcc ttgatgccaa tctcgacgaa ctggactaca gcggtgtgaa
    16561 gaccaagccc gccggagtcg agcgcaagac aggccacatc ggcttcgcgg gtcacagtga
    16621 tcccgtggcc ttccgcaact tccgcgtgaa gcggctctga gccgtcctcc cactgccttc
    16681 catcacagcc cgtccctaac cggacgggtt ttttctcatc ctgccctgac tcctccgcag
    16741 gcgcggcaaa gcaatacaga cagttttagg attgcctccc atttcatggg cgataatatg
    16801 cgcgatgcca tgtcagacga aatctaccca caagactcct cccctgactc acctccaccg
    16861 cgcaccacgg ggggcaactg gcgctgggag ccgcccagcc cggaggagct gcagaagctc
    16921 atgcccggct acaccattga gaagctgctg ggccgcggcg gcatgggcgc ggtctatcgc
    16981 ggcatccaga tgaacctgga ccggcccgtg gccatcaaga tccttccgcc cggcgtggag
    17041 aaggaggact ccagctttgc cgagcgcttc aagaatgagg ccaagctcat ggccaagctc
    17101 aaccaccccg ccgtggtcag cgtgcatgac tttggcgcca cccagggcgg gcagctctac
    17161 tttgccatgg agtatgtgga cggcagcgat gtctcgcaga tgatcgccgc gcaggggaaa
    17221 ctccctccgg accacgcgct ggccatcacc gctcatgtct gcgatgcgct ggccgccgcg
    17281 cacgagctgg gcatcgtgca tcgggacatc aagcccgcca acgtgcttat caacatgaag
    17341 gggcaggtga aggtggcgga ctttggcctg gcaaaggtgg aggagcctgg ccagcacggg
    17401 ttcaccaaga ccggctacgc catgggcacg ccggactttg tggcgccgga ggcgctgatg
    17461 ctgggcatgg ccatcgatgg ccgcgcggac atctacgccg tgggcgtgat gctctaccag
    17521 atgctgacgg ggaacatccc gcgcggggcc ttcaagtcgg ctgcgcagct ggtgccgggc
    17581 ctggacccgc ggtttgacgc catcatcacc aaggccatgc aaagcgcccg cgaggaccgc
    17641 caccagagcg ccgcggagct gcggcgtgag ctggatgtga tcctgaccgt ccccctggtg
    17701 cggcaggacg cgccggtcag cgcggccatc cccgtggccc aggtggcgca ggcccccggc
    17761 cagcgcagc
//
LOCUS       DAOMYJ010000091        17539 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Prosthecobacter sp. isolate AMR_MDS_5629
            NODE_1117_length_17539_cov_19.852437, whole genome shotgun
            sequence.
ACCESSION   DAOMYJ010000091 DAOMYJ010000000
VERSION     DAOMYJ010000091.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867850
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prosthecobacter sp. (wastewater metagenome)
  ORGANISM  Prosthecobacter sp.
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Verrucomicrobiaceae; Prosthecobacter.
REFERENCE   1  (bases 1 to 17539)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17539)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.30
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:34:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,742
            CDSs (total)                      :: 4,684
            Genes (coding)                    :: 4,676
            CDSs (with protein)               :: 4,676
            Genes (RNA)                       :: 58
            tRNAs                             :: 55
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17539
                     /organism="Prosthecobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1117_length_17539_cov_19.852437"
                     /isolate="AMR_MDS_5629"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1965333"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1392)
                     /locus_tag="PLP58_11105"
     CDS             complement(<1..1392)
                     /locus_tag="PLP58_11105"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HRJ09110.1"
                     /translation="MLELTGVSRTVPHKRGTLTVLNEASFCVPPGHLMTVIGATGSGK
                     TSLITLLAGHDAPSQGAILFQGRDCAVEAPHPNSIGHVPASDDVLCEALTVRESVMSA
                     LMLRVAGQSREERVARASHLLVGTGLETAATARVGTLTLPQKRRLKLALALVSDPALV
                     LCDEFTDGLDARSERELAALLKFVVGDHPARVVIHATQTVGNLAAYDTVVILHEGHVC
                     FHGPSRAVTHYFSIPAMDELYPRLAKRPAERWGDSWCRHRDSYYEAFKLGGLGEPATS
                     LGDEESAAARKAGAGRTGFTESRTAAAAPEPPPLPSATAQARHLMLRRWTLMRRTRAQ
                     WLRHLGVLLLCPAAAVLLVLPNRGFLEKLSADAGPEVLWPAAYTCMMALWVQILLVLM
                     MAVRAGAREIGGERPLYERERAGGLRPGAYLAAKLGFVLPLVLLQSLALGVLADMLTG
                     GLPGHALPRLLLLA"
     gene            complement(1459..2676)
                     /locus_tag="PLP58_11110"
     CDS             complement(1459..2676)
                     /locus_tag="PLP58_11110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009963846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HRJ09111.1"
                     /translation="MTPLFRKFIGINWILFLIMGGLLAFGVYAIFTATHFREDDDGLG
                     LALKWRDQLRWIGLGAPIFFAAALMDYKWLRWACLPIYLAGIGGLVYLDMYGVEVKGN
                     KAWVYIGGMSVQPSQFAILGGIIMLAVVFGELPRIWPVFRRPWLRILLAAVVAGIPGA
                     MVIKEDFGSGLVWGPVFLSMMLVGSIPFRYLITLVLGALCVTPLVYFFVLKDYQQARI
                     DTTWHMLTNQMEKVNVRGDGWVPNFVQIAVASAGFEGKRAARQVLGEDAVRTVHTSFF
                     PKTEAHSDFLFGVISEEFGFRGSVLLLTSMALLLVWCVFMAFYSRDQMGRLLIVGVVA
                     MFFAHTFQNAGMCVNLVPVIGLPMPFISYGGTFMIVTLFLMGMVQSVWVHRNISPLNK
                     KTSGGRSRRDEED"
     gene            complement(2701..3486)
                     /locus_tag="PLP58_11115"
     CDS             complement(2701..3486)
                     /locus_tag="PLP58_11115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009963847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HRJ09112.1"
                     /translation="MPLVRVENLQVHFPVRSGWLRPAGVVKAVDGVSFAVEEGRTLGL
                     VGESGSGKSTVSRALLKLIKPTGGAAWYRDREILGMSEAEFRPLRREMQMVFQDPIGS
                     LNPRMTIEAILGEPLGIHFKDMDRAGRRDVSAALLHRVGLPEDSLRRYPHEFSGGQRQ
                     RVGIARALAVRPKFLICDEPVSALDVSVQASILNLLKDLQDEFKLTYLFIAHDLAVVR
                     HMSDEILVMNGGRVVEAGPAEEVCGQPRHDYTRRLLASIPALG"
     repeat_region   3830..7109
                     /inference="COORDINATES: alignment:crt:1.2"
                     /inference="COORDINATES: alignment:pilercr:v1.02"
                     /rpt_family="CRISPR"
                     /rpt_type=direct
                     /rpt_unit_range=3900..3935
                     /rpt_unit_seq="ccgcctcaagctctcctgaatcgtggcgactgatgc"
     gene            complement(7461..7748)
                     /gene="cas2"
                     /locus_tag="PLP58_11120"
     CDS             complement(7461..7748)
                     /gene="cas2"
                     /locus_tag="PLP58_11120"
                     /inference="COORDINATES: protein motif:HMM:NF021356.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - endoribonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated endonuclease Cas2"
                     /protein_id="HRJ09113.1"
                     /translation="MRTRYLVSYDICHPKRLRRVAKAMEGYGVRLQYSVFECALDEKR
                     VNKMKAELQPILNHDEDQVLFVSLGPEDSSHGLRIEAMGLPYLARSRVTIV"
     gene            complement(7761..9587)
                     /gene="cas1"
                     /locus_tag="PLP58_11125"
     CDS             complement(7761..9587)
                     /gene="cas1"
                     /locus_tag="PLP58_11125"
                     /inference="COORDINATES: protein motif:HMM:TIGR00287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004520 - endodeoxyribonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated endonuclease Cas1"
                     /protein_id="HRJ09114.1"
                     /translation="MNSAAPIQTQDDALSLQPPLPVRRLHNFIYCQRLFYYQWVENLF
                     EENADTIAGSHAHRNVDKPSNYEEDKKVALAEGLPEGARLRSLKLESVTLGLVGVVDL
                     IEGGPEGAEVVDYKKGSAKRGQNGERVAKESDAVQVMAHALLLEEEGTRVTRGWIYYA
                     EDKRRVSVELSPELREQTLKALADAKALAASGHCPPPLVHDPRCLHCSAYPICLPNES
                     RWWAAGFEEKVDEQQMAFVFDGWPVAADASRWRPGGHALAGAATTAPEIKEAPRPDGD
                     AGEILVVQEPGAQVGQRGDMFTVSVKGEVVQKMPGHQLRAIYLYGAVQLTAQAVQTAL
                     ELDIDVAYFSPAGRFLGMLRGLPTSGVDARMGQYEFFQKEAVRLRLAREIIRAKIHNQ
                     RVLLMRNGDAPESVIRKMAELRDGVAGVEDRTQLLGYEGMAANFYFEHFPTMLKGRGE
                     AWRFDFQGRNKRPPRDPVNALLSLAYSMLAKELTGVCHAVGLDPFLGFLHQPRYGRPA
                     LALDLMEEFRPLIADSVVISLINRGELGVDDFVRSANGTFLKDAGRRAFWEGYFRRMD
                     TEVSHPEFGYKMSYRRMLEVQARQLWRYLRGEAAGYHGFTTR"
     gene            complement(9619..10014)
                     /locus_tag="PLP58_11130"
     CDS             complement(9619..10014)
                     /locus_tag="PLP58_11130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ09115.1"
                     /translation="MTLAAAFDLLREFHKRAPFLFFNGNTFAAIGRELSYALFSDLPP
                     VRKREVGSATAHYIAGVLDRTVMVQVVESLCAAADLAPGTRVRTLRGSTHGVVLRVLE
                     DGRIVWRTDSGTELIALPEALAAEEGGTA"
     gene            complement(10011..10247)
                     /locus_tag="PLP58_11135"
     CDS             complement(10011..10247)
                     /locus_tag="PLP58_11135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ09116.1"
                     /translation="MKLRLDLLKHLTTEDVIREAEATVHRFKPEPLFSTTGTGSLSSA
                     STQERAREVAHSTALTQSLEQRARQSGQSGTDKK"
     gene            complement(10290..10667)
                     /locus_tag="PLP58_11140"
     CDS             complement(10290..10667)
                     /locus_tag="PLP58_11140"
                     /inference="COORDINATES: protein motif:HMM:NF014043.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF86 domain-containing protein"
                     /protein_id="HRJ09117.1"
                     /translation="MSRHDPRLTLTQIREAARRAHLLCEGTSLDELRRDWIRLTAFER
                     QFEIIGEAIKRLPLELRERYPQHDWRGAAGLRDIVAHGYDWIDHEVLWDAVRGDFPSL
                     FTTVEEMMADLGGELPVEEAPLT"
     gene            complement(10654..10962)
                     /locus_tag="PLP58_11145"
     CDS             complement(10654..10962)
                     /locus_tag="PLP58_11145"
                     /inference="COORDINATES: protein motif:HMM:NF014021.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HRJ09118.1"
                     /translation="MKTLEELPIKIDREKIAAFCRERGIRKLSLFGSVVRDDFDPERS
                     DVDVLVEFLPGRKPGYGFVHCREALEELLGRPVDLLTGLGRRLGPLVKDDLSPIYEQA
                     "
     gene            complement(11062..11268)
                     /locus_tag="PLP58_11150"
     CDS             complement(11062..11268)
                     /locus_tag="PLP58_11150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ09119.1"
                     /translation="MSTLPQTLFTEIESAPEPVQREVLDFVVFLKTRGAAAPEGAESL
                     LPLAGRAWDADWSTPEEDAAWRDL"
     gene            complement(11328..15569)
                     /gene="cas12b"
                     /locus_tag="PLP58_11155"
     CDS             complement(11328..15569)
                     /gene="cas12b"
                     /locus_tag="PLP58_11155"
                     /inference="COORDINATES: protein motif:HMM:NF033949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type V CRISPR-associated protein Cas12b"
                     /protein_id="HRJ09120.1"
                     /translation="MNRIYQGRVTKVQVPAPAAAEETVGRKKAKKDAKPEWLDLPDGE
                     AALWRHHELFQDAVNYYTLALVALGDELPETHPFRKLRERMRQAWDEFPKRTPTSAIT
                     LGQSLRKWLGLDAKASLDDACRKIVELEKVSAALASQTAALLADDLSKTSDLGQYGRS
                     AAPRYVKPSYEGNFRDESNRKALEAVEFRLAVHEVVQRPLSEFGALSLATESQRENRG
                     SKAGEEARETLLKHVKKLKKESLLKDDEADDLTAQIKKLATDALLVIPAYEAGGDSKE
                     KSLGIPALLLGKQLGFGKAIMRCLTAAIPRPADNWQKGLDEARAAVKRGEDPLRMARG
                     ERGFVFLAFTALPAWSDAADGEPVWIEFDIAAFKEALMTINQFNEKTKEREEKKIEVK
                     AELDFMLGRNESWKPKTSTEEDDREVPVLAGDPRYEKLLALLRDMDEDRSAHALEEII
                     GPTQSALRGFGKLRATWMDLMREELDLPAEAVLQQAVTDLQREHKLDMGHTDFFLKLC
                     EKGNWDIWRDASDGEAADQLAHGWAKNVVYAAADAQELADEWKRLQESIRYTPAEPQY
                     SRRLFMFSDIKNAEGINHVSPGIVEVCVAAENDRGLLEPRRLCLGYGAPRMVRDQLME
                     GGSSQWLQPMMKAMGLDDENRATLKKEPAVELMPDWVGRKRHLHFLLNFPVDIATEKL
                     VALVGKEARWQKQMNSAWENGKLKQRFHLLWDGMEVKDKQKPDQWWWDNEDTQREGFT
                     CLSIDLGQRRAADFALLHSTPFELDGSFIRIGEAGGKVWHTRLWRYRNPDQPGQAVSG
                     LGSLRLPGEDAEVFYRGKAGTELSGKKGRLADDDDYAEARALAEKLLHAGEKAEAWIG
                     TCAADNSFPEQNDKLVRLFLGALSRYRTWHRWSWQLRPEHADRWDRVFKKEIAKLPYF
                     TEWGAIAEQEVTPANALALQLLVAEKAGELRIFLEEALVKIAGRVVPLRSNTWRWVEC
                     GSDPAGKPLHQLLSDGDKPDERPWVRGQRGLSLKRIEQLETFRRGVLSLNRLMRHRPG
                     EKPDFGTETRGETLPDPCVVLTDKIVRMKEERVNQTAHMILAVALGVRLKAPGADGKL
                     RVEQNIHGEYEPIPGREPVDFIVLEDLSRYTTDKSRARAENSRLMKWCHRAINEKVKM
                     LAEPFGIPVVEVFASFTSKFDARTGAPGFRAGEVSLQERAQWNKAVEKDPRIAAVFAQ
                     LEAAVGKGVKHPRLLIPQQLGELFIAAKPVKIGADPKTFPKIRQADINAAVNIGLRAI
                     GGPNCFHAHPRVRVEREGSKGKGSKGKRGRTSENQPASGKWLTRRENKREKAQFGSAK
                     EISFMKLSEDSALLKESKTTLMHDPLGIASYGHASIKDCEHPLLAHNAAIFSRRKNEL
                     GQPNGAVARLEWAVCEAINAARIKAWEGKGGQARS"
     gene            complement(16162..17304)
                     /locus_tag="PLP58_11160"
     CDS             complement(16162..17304)
                     /locus_tag="PLP58_11160"
                     /inference="COORDINATES: protein motif:HMM:NF012296.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HRJ09121.1"
                     /translation="MQAYSDPFREARQETGVMKCPFHGEDIAMILRHEDVRRAAKDWR
                     TFSSDAPFRVPIPSEEAVRSMRQLPIETDPPEHTDYRAIVEPFFQRPKDPAMIARVEA
                     LLAGMIDEALARDSVEVVNDFALPAQSRALTYLLNVPESEADVWIGWGIHVFKVTGGE
                     FKKGTVLEDYLHRKFDEAQAAPGEDFFSALARAEFRGRGLAREEMMGFANLAFAGGRD
                     TIIHTISCALGHLAAHPADFEFLRADPRRVTLASEEFFRVFMPLTHIGRVCPADTDVR
                     GVTVKAGQRASLCWSSANFDETVFPDPGEVRLDRKPNPHLSFGFGAHLCLGAPHARLI
                     LRTLLRLCCERVKSISILEARERVEHEAAFDRVLGYDSLTVRMEAL"
BASE COUNT         3128 a         6007 c         5181 g         3223 t
ORIGIN      
        1 ggccagcagc agcaggcgcg gcagcgcgtg gccaggcagg ccgccggtga gcatgtccgc
       61 cagcacgccc agcgccaggg actgcaggag caccagcggc agcacaaagc cgagcttcgc
      121 ggcgaggtag gcgcccggcc tcaagccgcc ggcgcgctcg cgctcgtaga gcggacgctc
      181 gccgccgatc tcccgcgccc ccgcgcgcac cgccatcatg agcacgagca ggatctgcac
      241 ccacagcgcc atcatgcagg tgtaagccgc cggccagagc acctccggcc cggcatccgc
      301 ggagagtttt tccaggaagc ccctgttcgg cagcaccagc agcacggcgg cggccgggca
      361 cagcagcagc acgcccagat gcctcagcca ttgcgcgcgg gtgcggcgca tcaaagtcca
      421 gcggcgcagc atcaggtgcc gggcctgcgc ggtggcggag ggcaaaggcg gcggttccgg
      481 cgcggcggcg gccgtcctgc tttcggtaaa gccggtccgc ccggccccgg ctttccgggc
      541 ggcggcgctt tcctcatccc cgagggaggt ggccggctct cccaggccgc cgagtttgaa
      601 ggcctcataa tacgagtcgc ggtgcctgca ccacgagtcc ccccagcgct cggcgggccg
      661 cttggccagg cggggataaa gctcgtccat cgccgggatg ctgaagtagt gggtgaccgc
      721 gcgcgagggc ccgtgaaaac agacgtgccc ctcgtgcaga atgaccacgg tgtcgtaggc
      781 ggccagattg ccgaccgtct gcgtggcgtg gatgacgacg cgcgcggggt ggtcccccac
      841 cacgaatttg agcagggcgg ccagctcccg ctcggagcgc gcgtccaggc cgtcggtgaa
      901 ctcgtcgcag agcacgaggg cgggatcgct gaccagggcg agcgcgagct tgagccggcg
      961 tttttgcggc agtgtcagcg tcccgacccg cgcggtggcc gccgtctcca ggcccgtgcc
     1021 caccagcaga tgagaggcgc gggccacgcg ctcctcccgg ctctgcccgg ccacccgcag
     1081 catgagcgcg ctcatcacgc tctcgcgcac ggtgagcgcc tcgcacagca cgtcgtcgga
     1141 ggcgggcaca tggccgatgc tgttggggtg cggcgcctcc acggcgcagt ccctgccctg
     1201 gaaaaggatg gccccctgcg agggcgcgtc atggcccgcc agcagcgtga tgagggaggt
     1261 cttgccgctg ccggtggcgc cgatcacggt catcaggtgg ccgggcggca cgcaaaagct
     1321 cgcctcgttg agcacggtca gggttccgcg cttgtgcgga acggtgcggg agacgccggt
     1381 gagttcgagc atggcgccag gaaagcggaa aacccgcgcg cgggcaagcc tcaagatgag
     1441 gcggcccggc cggggcgctc agtcctcctc atcacgcctg gagcgcccgc cggaggtctt
     1501 cttgttgagc ggcgagatgt tccggtggac ccacacgctc tggaccatgc ccatgagaaa
     1561 aagcgtgacg atcatgaacg tccccccgta gctgatgaag ggcatgggca ggccgatcac
     1621 cggcacgagg ttcacgcaca tgcccgcgtt ttggaacgtg tgcgcgaaga acatggccac
     1681 cacgcccacg atgagcagcc tgcccatctg gtcgcgcgag taaaaggcca tgaagacgca
     1741 ccacaccagc agcagcgcca tgctggtgag cagcagcacc gagccgcgga agccgaactc
     1801 ctcgctgatc acgccgaaaa gaaagtcgct gtgcgcctcg gtcttcggga agaagctcgt
     1861 gtggaccgtg cgcaccgcgt cctcccccag cacctggcgc gccgcccgct tgccctcgaa
     1921 gccggcggag gccaccgcga tctggacaaa gttcggcacc cagccgtcgc cgcgcacgtt
     1981 caccttctcc atctggttgg tgagcatgtg ccaggtggtg tcaatgcgcg cctgctggta
     2041 gtccttgagc acgaagaaat acaccagcgg ggtcacgcac agcgcgccca gcaccagggt
     2101 gatcaggtag cggaagggga tgctgcccac cagcatcatc gaaagaaaca ccggccccca
     2161 caccaggccc gagccgaagt cctccttgat caccatcgcc cccgggatgc cggccaccac
     2221 agccgccagc aggatgcgca gccacggccg ccggaacacc ggccagatgc gcggcagctc
     2281 cccaaacacc accgccagca tgatgatgcc gcccaggatc gcgaactgcg agggctgcac
     2341 cgacatgccg ccgatgtaca cccaggcctt gttccccttc acctccaccc cgtacatgtc
     2401 gaggtaaacc aggccgccga tgcccgccag gtaaatgggc aggcaggccc agcgcagcca
     2461 cttgtagtcc atcaaagccg ccgcgaagaa aatcggcgcg cccagcccga tccagcgcaa
     2521 ctggtcccgc cacttcagcg ccagacccag gccgtcatcg tcctcgcgga aatgcgtggc
     2581 cgtgaagatg gcatacacac cgaaggccag cagcccgccc atgatgagaa acaggatcca
     2641 gttgatgccg atgaatttgc ggaaaagcgg ggtcatggtc gcggcggtgg gggggaaggc
     2701 tcagccaagc gccgggatcg aggccagcag gcggcgcgtg taatcatgcc ggggctggcc
     2761 gcacacctcc tcggcggggc cggcctccac cacccggccg ccgttcatca caaggatctc
     2821 atcgctcatg tggcgcacca ccgccaggtc gtgggcgatg aacagatatg tcagcttgaa
     2881 ctcgtcctgc aaatccttca gcaggttcaa aatggacgcc tgcacgctca catccagcgc
     2941 gctgaccggc tcgtcgcaga tcaaaaactt cggacgcacc gccagcgccc gcgcgatgcc
     3001 cacgcgctgc cgctgcccgc cgctgaactc gtgcgggtag cgcctcagcg agtcctccgg
     3061 caggcccacc cggtgcagca gcgccgccga gacatcccgc ctccccgccc ggtccatgtc
     3121 cttgaagtga atgcccagcg gctcccccag gatcgcctcg atggtcatgc gcggattcag
     3181 cgagccgatc gggtcctgga acaccatctg catctcccgc cgcagcgggc ggaactccgc
     3241 ctcgctcatg cccaggattt cccggtccct gtaccatgcc gcgccgccgg tgggcttgat
     3301 cagcttcagc agcgcgcgcg agaccgtgga cttgccgctg ccgctctccc ccaccaagcc
     3361 cagcgtcctg ccctcctcca cagcgaaaga caccccgtcc acagccttca ccacgccggc
     3421 ggggcgcagc cagccggagc ggacggggaa atggacttgc aggttttcga ctcggacaag
     3481 gggcatgagc ggcggactct aacacaaccc cgccccatgc caacgcaaac accgccaatt
     3541 tccaggcgcg cggcgctgaa aagcggcaaa cccctcatgg aactgccgcc ccaaaagcgg
     3601 gacaagccgg atcagaccct gcaacctgcc cccgcagccg caaagctggc gagccccctg
     3661 cgaaaccggt tggaaacacc acttgcgcgg cgatgcccca ctcacgcgca acaccaggtg
     3721 accggggccg gcaaaaaaat tcgcgcggca tggcagtcag gccgtcggaa tacccccatc
     3781 ccattcacac gcaacaccac ggcctgactg ccatgccgcg cggctcaaag tcttgagcct
     3841 ctgatgcagg cgtggcgact gatgcgcact gaaaacggtt atggtaacgg tccgcaggac
     3901 cgcctcaagc tctcctgaat cgtggcgact gatgctgggc agggacttgt agttgtgcgc
     3961 ctcgtccatc cgcctcaagc tctcctgaat cgtggcgact gatgcagata gagacctgcc
     4021 ttttcggcat ccttcatctt ccgcctcaag ctctcctgaa tcgtggcgac tgatgcatgg
     4081 tgcgggatgc gtagtcatac tccgccttca cccgcctcaa gctctcctga atcgtggcga
     4141 ctgatgcatg tgctggatgg ggcggtgacg accctggagg aaccgcctca agctctcctg
     4201 aatcgtggcg actgatgcgc acacggcaac gggaaaaact gcaatctcaa gccgcctcaa
     4261 gctctcctga atcgtggcga ctgatgcccg ccaccgccgc cgccaagacc gcgctggaca
     4321 accgcctcaa gctctcctga atcgtggcga ctgatgcccg tctttaactc gccgaatttt
     4381 ggcgcatgac accgcctcaa gctctcctga atcgtggcga ctgatgcgcc cacgcgtcga
     4441 aaacgtagcc tccgggtgaa taccgcctca agctctcctg aatcgtggcg actgatgcac
     4501 catggaagag atcaccgtca ccatccaccc cccgcctcaa gctctcctga atcgtggcga
     4561 ctgatgcggg gtgacggtga cgttgcgggc ggtggtggcg ggccgcctca agctctcctg
     4621 aatcgtggcg actgatgcgc taggttgtag ccttttccat aaccgaactc ctcccgcctc
     4681 aagctctcct gaatcgtggc gactgatgcc cgatgctccg cgcggtcaca gcggccgcgc
     4741 cgttccgcct caagctctcc tgaatcgtgg cgactgatgc ctgcctcaat gtaaggcaag
     4801 atagcacatg cctcccgcct caagctctcc tgaatcgtgg cgactgatgc ggagatgacc
     4861 tgctgcattt gatgcaggtc ctcgcgccgc ctcaagctct cctgaatcgt ggcgactgat
     4921 gcttgcggcg tcacgatccc ccatgttttc ttctcatccg cctcaagctc tcctgaatcg
     4981 tggcgactga tgccgagcgc tgtgtgtccg tgagtggaaa gaaaacgccg cctcaagctc
     5041 tcctgaatcg tggcgactga tgcccaccgt gatctgcgcg ttgtagcgca gcacgagccg
     5101 cctcaagctc tcctgaatcg tggcgactga tgctgattag aaactaggtg aactatatta
     5161 gttcctagcc gcctcaagct ctcctgaatc gtggcgactg atgcttgctt taccgagctt
     5221 tccatacccg tagtcaccca accgcctcaa gctctcctga atcgtggcga ctgatgctga
     5281 aaaaggggaa agggagaaag agaaaaagga gtccgcctca agctctcctg aatcgtggcg
     5341 actgatgcgg ggcagtacga cggcacggaa tactctcgtc accgcctcaa gctctcctga
     5401 atcgtggcga ctgatgcacc gctcgatgta gctgcgcacc ggctcccaca ccgcctcaag
     5461 ctctcctgaa tcgtggcgac tgatgcgggg cagtacgacg gcacggaata ctctcgtcac
     5521 cgcctcaagc tctcctgaat cgtggcgact gatgcaccgc tcgatgtagc tgcgcaccgg
     5581 ctcccacacc gcctcaagct ctcctgaatc gtggcgactg atgcctggcg ctttacgcca
     5641 tctacaagct ggccgcttgc cgcctcaagc tctcctgaat cgtggcgact gatgccagac
     5701 agctacgagc ggttcactgc atccgctgcc gcctcaagct ctcctgaatc gtggcgactg
     5761 atgccactgc atgtctgctc tatcaatagc ttagggtatc cgcctcaagc tctcctgaat
     5821 cgtggcgact gatgccctat gcaggctacc acggaaacaa aaacgcagac cgcctcaagc
     5881 tctcctgaat cgtggcgact gatgcattca gggggccttg ccgagaaaag tcagtgaggt
     5941 ccgcctcaag ctctcctgaa tcgtggcgac tgatgcctcg gcgactggta aacgcccagc
     6001 accttgtcgg cccgcctcaa gctctcctga atcgtggcga ctgatgcgct gacgcggaga
     6061 aatttcaggc ctttgccggt ccgcctcaag ctctcctgaa tcgtggcgac tgatgcctcc
     6121 gagccgttgg ctgcatctga tttgttcgtc ccccgcctca agctctcctg aatcgtggcg
     6181 actgatgctg gcggtcaaga tgttcggacc agggggaaaa ctcaccgcct caagctctcc
     6241 tgaatcgtgg cgactgatgc cgcgggtcgt gccggcgctg gtggaccgct gcatccgcct
     6301 caagctctcc tgaatcgtgg cgactgatgc cccggcgctg ggtagatgac tgcaaaccgt
     6361 tctcaccgcc tcaagctctc ctgaatcgtg gcgactgatg cgtcgagcgt ctgccggcgg
     6421 atttccagga gcttgaccgc ctcaagctct cctgaatcgt ggcgactgat gcttggtcag
     6481 cacgggggcc aaagaaatac aggtcacccg cctcaagctc tcctgaatcg tggcgactga
     6541 tgcccatcgg ccaggacgac cacggttttt ccctccaccg cctcaagctc tcctgaatcg
     6601 tggcgactga tgcccttatg tcgtgatgcc tcccgagcac tacttcctgc cgcctcaagc
     6661 tctcctgaat cgtggcgact gatgccacgt tatgaacgat tacgtcgaac ccaagatgaa
     6721 ccgcctcaag ctctcctgaa tcgtggcgac tgatgcggca gcggagatag tatcccagcg
     6781 cgaagcatac ccccgcctca agctctcctg aatcgtggcg actgatgcgg cgcgctcttg
     6841 cagcacccca atttgacccg ctcccgcctc aagctctcct gaatcgtggc gactgatgcc
     6901 aacgcgcaaa ggctgctggg tgcattcgca tctccgcctc aagctctcct gaatcgtggc
     6961 gactgatgca aggcctggcc ggtgatggcc tggggggcga gatccgcctc aagctctcct
     7021 gaatcgtggc gactgatgcc agcaagcaag ccatagatgg tgccagtcag ggaccgcctc
     7081 aagctctcct gaatcgtggc gactgatgcc gcgccgccgt ggaacgccca ttctacctgg
     7141 atacctgggc cgctgcgcga gcgccattcg tggaacgacc tcactttgga gtgaatcgcc
     7201 tgttagataa agtgtgacgc ccttcgcgag cgcggtgtga gcgcaggccc ggcacttccg
     7261 cgctcgcgag gagcgccgct catcagtcat cacgatatca aagacccgtg ccgcagcacg
     7321 tattttggag agattgcccg ttccaatggc ggaggaaggg tggtcggtgg cggaggcttg
     7381 cgctttgttc gccgctccat gctcgcggtt tccgccgcgg gcgcaagcaa gatttgggcg
     7441 ggaatgccat cccgcccgac tcaaacgatc gtcacccggc tgcgcgccag atacggcagc
     7501 cccatcgcct cgatccgcag gccgtggctg ctgtcctccg gtcccaggct gacaaagagc
     7561 acctggtcct catcatggtt gaggatcggc tgaagctccg ccttcatctt gttcacccgc
     7621 ttctcatcca gcgcgcattc aaagacgctg tattgcaggc gcacgccgta gccctccatc
     7681 gccttcgcca cccggcgcag gcgcttcggg tggcagatgt cgtagctgac caggtatcgt
     7741 gttctcatgg ccgtcctcct tcagcgcgtg gtgaatccat gataacctgc cgcctccccg
     7801 cgcaggtagc gccagagctg ccgggcctgc acctccagca tgcggcggta gctcatcttg
     7861 tagccaaact ccgggtggct cacctccgtg tccatccgcc tgaagtagcc ctcccagaag
     7921 gcccgccgcc cggcatcctt caggaaggtg ccgttcgcgc tccgcacaaa gtcatccacc
     7981 cccagctccc cccggttgat cagggagatc accacgctgt ccgcgatcag cgggcggaat
     8041 tcctccatca ggtccaaagc gagcgccggg cggccatagc gcggctggtg gaggaagccg
     8101 aggaacgggt ccagccccac cgcatggcac acgcccgtca gctccttggc cagcatcgag
     8161 taagccaggg acagcagcgc gttcaccgga tcacgcggcg ggcgcttgtt gcggccctgg
     8221 aagtcgaagc gccacgcctc cccccggccc ttgagcatgg tggggaaatg ctcgaagtaa
     8281 aaattcgccg ccatcccttc gtagccgagg agctgcgtgc ggtcctccac cccggccacg
     8341 ccgtcgcgca gttccgccat cttcctgatc acgctctccg gcgcgtcgcc gttgcgcatc
     8401 agcagcacgc gctggttgtg gatcttcgcg cggatgatct cccgcgccag cctcagacgg
     8461 acggcctcct tttggaaaaa ctcgtactgg cccatgcgcg cgtccacccc gctcgtcggc
     8521 agcccgcgca gcatccccag gaaccggccc gcaggggaga aataggccac gtcaatgtcc
     8581 agctccagcg ccgtctgcac cgcctgcgcc gtgagctgca ccgctccata aagatagatc
     8641 gcccggagct ggtgcccagg catcttctgc accacctccc ccttcacgct gacggtgaac
     8701 atgtccccgc gctgccccac ctgcgccccc ggctcctgca ccaccaggat ttcccccgca
     8761 tccccgtccg gcctcggcgc ttccttgatc tcgggggccg tcgtagctgc gcccgccagg
     8821 gcgtggcctc ccggcctcca ccggctggcg tcggcggcta cgggccagcc atcaaacaca
     8881 aacgccatct gctgctcatc caccttctcc tcaaagcccg ccgcccacca gcggctctca
     8941 ttgggcaggc agattggata cgccgagcag tgcaggcagc gcggatcatg caccagcggc
     9001 ggcgggcagt ggccgctggc cgccagcgcc ttcgcatcgg ccagcgcctt cagggtctgc
     9061 tcacgcagtt ccggcgacag ctccacgctc acccggcgct tgtcctccgc gtagtagatc
     9121 cagccgcgcg tcacccgtgt gccctcctcc tccagcagca gggcatgcgc catcacctgc
     9181 accgcgtcgc tctccttcgc cacccgctcc ccgttctgcc cgcgtttcgc gctgcccttc
     9241 ttgtagtcca cgacttccgc gccctccggc ccgccttcga tcagatccac cacgcccacc
     9301 agtcccagcg tcacactttc cagcttcagg ctccgcagcc gggctccttc cggcagcccc
     9361 tccgccagcg ccaccttctt gtcctcttca tagtttgagg gcttgtccac gttccggtgc
     9421 gcatggcttc cggcgatcgt gtccgcgttc tcctcgaaga gattctccac ccactggtaa
     9481 tagaagaggc gctggcagta gatgaagttg tgcagccggc gcaccggcag cggcggttgc
     9541 agggacagcg cgtcatcctg cgtctggatc ggggcggcgg agttcatgac ggcgggtact
     9601 ctaaacccgc ctgtcaagtc aggccgttcc gccttcctcc gccgccagcg cctccggcag
     9661 ggcgatgagt tccgtcccgc tgtccgtccg ccacacaatg cgcccgtcct ccagcacccg
     9721 cagcaccacg ccgtgcgtgc tgccgcgcag cgtcctaacc cgcgtgcccg gcgccagatc
     9781 ggccgccgcg cacaggctct ccaccacctg caccatcacc gtccggtcca gcaccccggc
     9841 gatgtaatgc gccgttgccg agcccacctc acgcttccgc accggcggca gatcgctgaa
     9901 gagcgcgtag ctcagctcac gcccgatcgc cgcaaaggtg ttcccattga agaacaggaa
     9961 cggcgcccgc ttgtggaact cccgcagcaa gtcaaacgcc gccgccagcg tcatttcttg
    10021 tccgtgccgc tctgcccaga ctgccgcgca cgctgctcca gactctgagt taaagccgtg
    10081 ctgtgcgcca cctcgcgcgc acgctcctgc gttgatgcgg acgacagact gccagtccca
    10141 gttgtggaga aaagcggctc gggtttgaaa cggtgaaccg tcgcctccgc ctcccggatc
    10201 acgtcctccg tggtcaggtg cttcagcagg tcgaggcgga gtttcatcgc tcataacata
    10261 cgcacgtcag gggtttcgca aggcagggat caagtcagcg gagcctcctc caccggcagt
    10321 tccccgccca gatcagccat catttcctcc accgtcgtga acagggaggg gaaatccccc
    10381 cgcaccgcat cccatagcac ctcatggtcg atccagtcgt agccatgcgc cacgatgtcc
    10441 cgcaggccgg cggcaccgcg ccagtcgtgc tgcgggtagc gctcccgcag ctccagcggc
    10501 agccgcttga tcgcctcgcc gatgatttca aactggcgct cgaacgccgt gagccgtatc
    10561 cagtcccgcc tcagttcatc cagggatgtt ccctcacaga ggagatgcgc ccgccgcgcc
    10621 gcttcccgta tctgggtcag cgtcagccgt ggatcatgcc tgctcataga tcggcgagag
    10681 atcatccttc accagcggcc ccaggcgcct gcccagtccg gtgagcaggt ccaccggacg
    10741 ccccagcagt tcttcgagcg cttcccggca gtggacgaag ccgtaacccg gcttgcgtcc
    10801 cggcaggaat tccaccagca catccacatc actgcgctcc gggtcgaagt catcccgcac
    10861 cacgctgcca aacaggctca gcttccggat gccccgctca cggcagaacg ccgcgatctt
    10921 ctcgcggtcg atcttgatcg gcagctcctc cagcgtcttc atgccgccta gtttaagaga
    10981 tgcgtcagga ttggcaagac agggatcaag gcatcggaat ctcgtcggag cacgggatcg
    11041 caagcaccac cacctcatcg ctcacaaatc gcgccatgcc gcgtcctcct ccggggtgct
    11101 ccagtccgcg tcccaggccc ggcccgccag cggcagcagg ctctccgcgc cttccggcgc
    11161 ggcagccccg cgcgtcttga ggaagaccac aaagtccagc acctcccgct gcaccggttc
    11221 aggcgccgat tcgatctcgg tgaacaaagt ctggggcagc gtgctcatgc gcctagcata
    11281 cgggccgcac cgggattggc aaggcaggtc agagcgggat ttgatcatca ggatcgggct
    11341 tggccgcctt tgccctccca ggccttgatt cgcgcggcgt taatcgcttc gcaaacagcc
    11401 cactcaagcc gagcgacagc accattaggc tggccaagtt cgttttttcg tcggctgaag
    11461 atggctgcgt tatgcgccaa caaaggatgc tcgcagtctt tgatggatgc gtgtccgtac
    11521 gatgcaatac caagcgggtc gtgcatcaag gtcgttttgc tctccttgag caaggcggaa
    11581 tcttcggaga gcttcatgaa gctgatttct ttcgcgctgc caaactgcgc cttctcccgt
    11641 ttgttttcac gacgcgtgag ccactttccc gaagcaggtt ggttttcaga agtcctccct
    11701 cgtttcccct tgcttccctt gccctttgat ccttcgcgtt cgactcgcac tcgcggatgc
    11761 gcatggaagc aattggggcc gccgatggcg cgcaggccga tgttcaccgc cgcattgatg
    11821 tccgcctgtc gtatcttcgg aaatgtcttc ggatcggcgc caatcttcac cggcttggcg
    11881 gcaatgaaga gctccccaag ttgctgtgga atgagcaagc gtggatgctt cactcccttc
    11941 ccgacagccg cttccaattg cgcaaacacc gccgcgatcc tcggatcttt ctcaacggcc
    12001 ttgttccatt gcgcccgctc ttgcagtgaa acctcgcccg cacggaaacc cggcgcgccg
    12061 gtgcgcgcgt caaactttga ggtgaacgac gcgaacacct ccaccacggg aataccaaac
    12121 ggctcggcca gcatcttgac cttctcattg atggcgcgat gacaccattt cataagccgt
    12181 gagttctcgg cgcgggcgcg gctcttgtcg gtagtgtagc gggacaaatc ctccagcacg
    12241 atgaagtcca ccggctccct gcctgggatc ggctcatact cgccgtggat gttctgctcg
    12301 acacgcagct tgccatccgc gcctggtgcc ttgagccgca ctcccagggc cacggccagg
    12361 atcatatgcg ccgtctgatt caccctctcc tctttcatgc gcacgatctt gtcagtaagc
    12421 acgacgcagg gatcaggcaa ggtctcgcct cgcgtctcgg tcccgaagtc aggcttctcg
    12481 cccggacgat gacgcatcag gcgattgagt gaaagcacac cgcgccgaaa agtttcaagc
    12541 tgctcgatgc gcttcaacga caagccgcgc tgtcctctga cccacgggcg ttcatctggc
    12601 ttgtcgccat cggagagcaa ttggtggagc ggtttccctg ccggatcact cccgcattcc
    12661 acccagcgcc aggtgttgct tcgcaatgga accacccgac cagcgatttt gaccaacgcc
    12721 tcctccagaa agatgcgcaa ttccccggct ttctccgcca ccagcagttg cagggccagc
    12781 gcattagctg gcgtcacttc ctgctcggct atggcacccc attcagtaaa atagggcagc
    12841 ttggcaatct ccttcttaaa cacgcgatcc cagcggtcgg cgtgctcagg acgcaactgc
    12901 cacgaccagc gatgccaggt gcggtaacgg ctcaaggcgc cgagaaacag ccgcaccagt
    12961 ttgtcgttct gctcgggaaa agagttgtcc gcagcgcagg tcccaatcca tgcctcggcc
    13021 ttctcgccgg cgtgcagtag cttttccgcc agcgccctgg cctccgcgta gtcgtcatca
    13081 tccgcgagcc tgcctttctt gcccgacagt tcggtccctg ctttcccgcg gtaaaacacc
    13141 tccgcgtcct caccgggcag gcgcaaactg ccgaggcccg acaccgcctg ccccggctgg
    13201 tctggatttc ggtacctcca taggcgcgtg tgccagacct tgccgccagc ctcgccgatg
    13261 cggataaacg atccatccaa ctcgaacggc gtggaatgca gcagcgcaaa gtccgccgcc
    13321 ctgcgctggc caaggtcgat ggacaggcag gtgaatcctt cgcgctgagt gtcctcgttg
    13381 tcccaccacc actgatccgg cttctgtttg tccttcacct ccattccatc ccagagaaga
    13441 tgaaaccgtt gcttgagctt cccgttctcc caggcggagt tcatctgctt ctgccaccgc
    13501 gcttctttgc cgaccagggc aaccagcttc tctgtggcga tgtccaccgg gaagttgagc
    13561 aagaaatgca ggtggcgttt ccgccccacc cagtcaggca tcagttcaac ggcaggctct
    13621 ttctttaatg tggcgcggtt ttcatcatca agtcccatcg ccttcatcat cggttgcagc
    13681 cattgggaag agccgccctc catcaactga tcccgcacca tgcgcggcgc gccataacca
    13741 agacacagcc gtcgcggctc cagcagccct cgatcattct ctgccgcgac gcagacctcg
    13801 acaatgccgg gtgagacatg gttgatcccc tcagcattct tgatgtccga gaacatgaac
    13861 aagcggcgcg aatactgcgg ctcggctggc gtgtagcgaa tagactcctg caaccgtttc
    13921 cactcatccg ccagttcctg ggcatccgcc gccgcataca cgacattctt cgcccaacca
    13981 tgcgcaagct ggtccgcagc ctcaccgtcc gaggcatccc gccagatgtc ccaattcccc
    14041 ttctcgcaca gcttgaggaa gaaatccgtg tgccccatgt ccagcttgtg ctcccgctgc
    14101 agatccgtga ccgcttgttg cagcacggcc tccgctggca aatccagctc ctcgcgcatc
    14161 aagtccatcc aagttgcgcg cagcttcccg aagccacgca gcgcggattg agtcggcccg
    14221 atgatctctt ccaaagcatg ggcggaccgg tcctcatcca tgtcgcgcag gagagccagc
    14281 agcttttcat aacgcgggtc gcccgccaac accggcactt cccggtcatc ttcctcggtg
    14341 ctggttttgg gcttccacga ctcattgcgc cccagcatga aatcgagctc agccttcacc
    14401 tcgatcttct tctcttcccg ctccttggtc ttctcgttga attgattgat tgtcatcagc
    14461 gcctctttga aggcggcgat gtcgaactca atccagaccg gctccccatc agcggcatcg
    14521 ctccaggctg gcagcgcggt aaaggcgaga aagacgaagc cacgctcgcc gcgtgccatt
    14581 cgcaacgggt cctccccgcg cttcaccgca gctcgggctt cgtcgagccc cttttgccag
    14641 ttgtcagcgg gacgcgggat ggcggcggtc aggcagcgca ttatcgcctt tccaaatcca
    14701 agttgcttgc ctagcagcag cgccgggatg cccaaggact tctccttgct gtctccgcct
    14761 gcttcataag ccggaatcac caacaaggca tctgttgcca gcttcttgat ctgtgccgtc
    14821 aaatcgtctg cctcgtcatc tttcaacaag ctttccttct tcaacttctt gacgtgcttc
    14881 aacagtgttt cgcgcgcttc ttctccagcc ttgctgccgc gattctctcg ttgcgactcc
    14941 gttgccagcg atagcgcgcc aaattcagac aaagggcgct gaaccacttc atgaactgcg
    15001 agcctgaact ccactgcctc caatgctttc cggttggact cgtcgcggaa atttccctcg
    15061 taagagggct tcacatagcg cggtgcagcg gaccggccgt attgccccaa gtccgaagtc
    15121 ttgctcagat catccgccag caaagcggcc gtctgcgaag ctagagcggc ggaaaccttt
    15181 tcaagctcta cgatcttacg gcacgcatca tcgagcgaag ccttcgcatc cagtcccaac
    15241 cacttccgca acgattgccc taatgtgata gcggaagttg gagtccgctt cggaaactca
    15301 tcccaagcct ggcgcattcg ttctcgtaac tttctaaaag gatgggtctc gggcaactca
    15361 tcacccaacg ccacgagcgc aagcgtgtaa taattcaccg catcctgaaa cagctcatgg
    15421 tgccgccaca gcgcggcttc gccgtcgggc aggtcgagcc attccggttt tgcgtctttt
    15481 ttcgccttct ttcggccaac tgtttcttct gcggcggctg gcgcgggcac ttgcaccttc
    15541 gtgacacgtc cttggtaaat tcggttcatg gcgggaaaat cggggtttta gcggcgcgag
    15601 tttgggctgg agggtgggac aatggcgggg ggaggggcaa tgtttttttt cgtttgcggc
    15661 ggcgcggcgg ggcggggcgc gcccgcggcg gcgggttttt ttcaacattg cgggcggcgg
    15721 gcatcgtcaa aggcggcaat ccccatgaag acgaaccaca cgcgggcggc cctggcgggg
    15781 agggcggctt ccagggctgc ggcgcgcggc gagagattcc ggcgaatgat gccaaagcat
    15841 gagccttgcc cggcgcgctc cgtcttccac ggtgaaaggg tgcgattctt gatccgccgg
    15901 cacttttttc ttcacatccg cgcgtgtttt tgattcactc accgccccaa accacctgga
    15961 tgggaggcac gggggtgagg tgcgggcgct ggcacgcttg agcgctgttg ccatgacggg
    16021 ggcaaactga cgtttgcctc tacgggcggc gcggggggcg cgggtagagg aagacgtgag
    16081 tcttccccgg atgggaggcg cgggcggaat ggcgcgtctg tgccgggagc ggggcaaact
    16141 gacgtttgcc tctgcgggcg gtcacagcgc ctccatgcgc acggtgaggg agtcgtagcc
    16201 gaggacgcgg tcgaaggcgg cctcgtgctc gacgcgctcg cgcgcctcga ggatggagat
    16261 gcttttcacc cgctcgcagc acaggcgcag gagggtgcgc aggatgaggc gggcgtgcgg
    16321 ggcgccgagg cagaggtgcg cgccgaagcc gaaggagaga tgcgggttgg gtttgcggtc
    16381 gagccgcacc tcgccggggt cggggaagac ggtctcgtcg aagttggcgg aggaccagca
    16441 gagggaggcg cgctggccgg ctttgacggt gacgccgcgg acatcggtgt ccgccgggca
    16501 gacgcggccg atgtgggtga ggggcatgaa gacgcggaag aactcctcgc tggcgagggt
    16561 gacgcgcctg gggtcggcgc ggaggaactc gaagtcggcg gggtgcgcgg ccaggtggcc
    16621 gagggcgcag gagatggtgt ggatgatggt gtcgcgcccg ccggcgaagg cgaggttggc
    16681 gaagcccatc atctcctcgc gcgccaggcc gcgcccgcgg aattcggcgc gcgccagggc
    16741 gctgaagaaa tcctcccccg gcgcggcctg cgcctcgtcg aacttccggt gcaggtagtc
    16801 ctccagcacg gtgccttttt tgaactcgcc gccggtgact ttgaagacgt ggatgcccca
    16861 gccgatccag acatcggcct cggactcggg cacgttgagc agataggtca aagcgcggga
    16921 ttgcgcgggc agggcgaagt cgttgaccac ctcgaccgag tcccgcgcca gggcttcgtc
    16981 aatcatgccg gcgagcaggg cctcgacccg ggcgatcatg gcggggtcct tcgggcgctg
    17041 gaaaaacggc tcgacgatgg cgcggtagtc ggtgtgctcg ggcgggtcgg tctcgatggg
    17101 cagctggcgc atggagcgca cggcctcctc ggaggggatg ggcacgcgga agggggcgtc
    17161 ggagctgaaa gtccgccagt ctttggcggc gcggcgcaca tcctcatggc ggaggatcat
    17221 ggcgatgtcc tcgccgtgga aggggcactt catcacgccg gtttcttggc gcgcttcgcg
    17281 gaaggggtcg gagtaggctt gcatgctcgg aggccgggaa ataggcagcg gaaggcggcg
    17341 cggtcatttt ctttttgcgc gcgcgtatcc gcgcgcctgg catgaccgcc gctcatgccg
    17401 tctccagcag cctgccgatc tggccgaagt atcccgccgc catttcaaag atgcgccaca
    17461 ccacatagac gaccaccagc gcgtagcccg cctttggcag ccacgcggcg gcgcggtcca
    17521 cggcagcctc ggcgtccgc
//
LOCUS       DAOMYJ010000092        17513 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Prosthecobacter sp. isolate AMR_MDS_5629
            NODE_1120_length_17513_cov_22.136785, whole genome shotgun
            sequence.
ACCESSION   DAOMYJ010000092 DAOMYJ010000000
VERSION     DAOMYJ010000092.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867850
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prosthecobacter sp. (wastewater metagenome)
  ORGANISM  Prosthecobacter sp.
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Verrucomicrobiaceae; Prosthecobacter.
REFERENCE   1  (bases 1 to 17513)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17513)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.30
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:34:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,742
            CDSs (total)                      :: 4,684
            Genes (coding)                    :: 4,676
            CDSs (with protein)               :: 4,676
            Genes (RNA)                       :: 58
            tRNAs                             :: 55
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17513
                     /organism="Prosthecobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1120_length_17513_cov_22.136785"
                     /isolate="AMR_MDS_5629"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1965333"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..474
                     /locus_tag="PLP58_11165"
     CDS             <1..474
                     /locus_tag="PLP58_11165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ09122.1"
                     /translation="LALGLVVVGVQTGIGAARVAPPDLRLFGMSPKEFLVVPLGSIMM
                     FGALVCAGVAARRRPAAHRPLMFMASLAAVSAALGRIGPLNTWYAGTWLERGFSAFLT
                     MLLLGALVLGVKWGVTRRFDGWFASVLAALAVCSMALSLGSKTGAWDSFATFLLR"
     gene            499..1011
                     /locus_tag="PLP58_11170"
     CDS             499..1011
                     /locus_tag="PLP58_11170"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009963132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004725 - protein tyrosine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight
                     protein-tyrosine-phosphatase"
                     /protein_id="HRJ09123.1"
                     /translation="MPAASDRPFRLLFVCMGNICRSPAAEGVMRRLTREAGLAEQVEI
                     DSAGTGGWHEGSPPDDRMTAAARRRGYELTGRARQITAGDIRRFDLVLIMDEQNRRDI
                     RPWLPDGPEREKVRMFCEFCARFENDEVPDPYYGGREGFDRVLDLLEDGCAGVLEHIR
                     QWRAGAAEKP"
     gene            1069..5115
                     /locus_tag="PLP58_11175"
     CDS             1069..5115
                     /locus_tag="PLP58_11175"
                     /inference="COORDINATES: protein motif:HMM:NF024371.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3857 domain-containing protein"
                     /protein_id="HRJ09124.1"
                     /translation="MTLLLAEVETAQMTGQWIGSVLGILLLSAGLIKCLTLLRRPATS
                     KTCVSALALALAGWLGALLFQSIAFLVPEALAVVYVIGGLGMAGLMLAAIVTGITGLA
                     LYDSKVHTQGRAQAVWSLVIAVLMLTVAVTGLVLQMRLESAANKLASLSGKGAEIRSA
                     EFNCSLEIPPRWLETKPALINKLACIAARRAAPEAYVMVLGEHIDGEMDLEALLDAVK
                     ANMAATSKSVTGETEESLTVDGVPFMRRACLVENDAAKGIKLYFEQWVSTLPGYAWQL
                     HMWGPVAGRADLEPQFKRIGESFRVLDKQRVVPASAALDNVSRPQWGYRTEFNAGDWK
                     KWGVEGHEQTLADFAALRPLEAFIVLPVNLGPEPPPLEAAASGLLDRFGIPFPSDDWP
                     ARKWPNAWGEGIEITGVRSLEDGDYSYRMRVAARGPVALLIAGWHAVKNGDPEMVRKA
                     VDGVVLLPGEPGEIPGLEESQRGQYGLVCNDIGIWHHQRQQTGKAAEWFWRGFQQTQK
                     DPTILGNALDAWQQTGRAAEALEKAGEHIKKFPAHVNTQLSHARLLCDLGRDDEAADA
                     FAAALKAGVKDPDAVLRWLQYLNGAEKYALAEKTAAAWMEQSGNLDSRRWHAQTVASN
                     GGKARAIQLFEKMVEEHPEDRRLLYELAETRNDAAEYLQAAELAQGILKDTPDSARAW
                     MILGWSQMGRKWYREAKKSFEEALEKQPDSEEIQQALRRASAMLGQGSNSDIKTPIEP
                     VALPEELRELLEKNPPAAGHAAGQPVAWLLSATGYHFEKDKPLRTTWHVRVKLRDSEG
                     VSRFSSVEHNFDPLGSRVFINSVEVRDEEGRVVASAAGDAYVMDVDNGMGTHDKKLHF
                     QIPGLRPGCVVEYVISKESRYKAETFEFNRHLFSDSAADIVFVTGDVDGVAFAGNAAL
                     ADLKHLRQDRLLAWTGLHLPQSTREPFPVLMEERVPCLALGGHEGSWAEVGDKLLDKE
                     LAGRLEPEAEVTALAQRLIQGVGSEGEKIAALARHVQQAISYTAIEFGTRARRPNPAA
                     QTLGQKYGDCKDQALLLHQLLTAAGIGSHLALVSNNWRTRVELPSVDQFNHMVVHVPA
                     LGPGFLIDTTDKNLALVSLHADNLWHSHALVLERGKSRLIEPAQPAPGSARILSERKV
                     RPSGDGWEVNESLVIHGYYAAWMRGAFTGKDAQRQLQNVQNILAAHAPALRVHEFTFH
                     HLEDTGLPARLEIAYEVPGRLQDEGALRRGALPALWEQEYLGTTFVKNRASAFRFIYP
                     LQFESDLRVEGAGGIPEDALKALNVSREGRFASWKMEAAAQGGGLLKLRFRFSTQPGT
                     YDAADYPDWHEQWNSAVSQWQRPFSWKP"
     gene            5167..6750
                     /locus_tag="PLP58_11180"
     CDS             5167..6750
                     /locus_tag="PLP58_11180"
                     /inference="COORDINATES: protein motif:HMM:NF013739.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HRJ09125.1"
                     /translation="MQCDGVILGSGHNALVLACYAARAGMSIVVVEKNDEAGGGLATV
                     EHPAGSGFMHNTHSFFHRAITTMPWYQDLGLERHGARYIEPDLNVAMILPDERVLEWW
                     TDFEKTFASFAAISPRDAARLREWTERFRPIVTDILIPEAQNPPLPMEERLSKLSQSQ
                     AGRLLLETQALSPIEFVMREFEHPAVRAGLLFFNGLREVDLRQKGFGHSIPALLASPR
                     KAQMCAGGSRRLAEALCAELRGHGGRILTGWQPRSILMRGGRACGVESLTGERVEAAR
                     FVASGLNPQQTFIDLIPADAAPSSLRERAAGFEYNLLAPLFALNLNLREPPAYTAAER
                     HPELSRAFMVILGLGDVTQFQEIVSAHERGEIPRTVMWGACPTLFDPSQAPPGRHTAF
                     MWEKLPYALRGDARNWDACKEAHGRVMLDLWAKHAPNLHDAVLGSFTRSALDTERLLP
                     NMMRGDLLVGSFANGQTGFHRPFKGAGSYRTEIPGLYLCGGSTHPGGNVTGLCGFNAA
                     RVLLADLGVERGTLSVVPP"
     gene            complement(6770..7312)
                     /locus_tag="PLP58_11185"
     CDS             complement(6770..7312)
                     /locus_tag="PLP58_11185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ09126.1"
                     /translation="MKTRFKGWVISAAVVLMTVGMPEAGANDDLQALFQMGRAAYYKG
                     DMEMAYQLLKQVEAKQPNHYETKVLLAQIRTHMKTGTGSLKKTYSGVTLAKIEFVDVS
                     LEEAVEGLRGLCKTSTEGKVMPNIIIKDAELKAKTLSLQLNNIPLTEAIEYIARVAGA
                     RAVYEQHAVMLVSLAGSGTP"
     gene            7435..10761
                     /gene="mfd"
                     /locus_tag="PLP58_11190"
     CDS             7435..10761
                     /gene="mfd"
                     /locus_tag="PLP58_11190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018969780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003690 - double-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HRJ09127.1"
                     /translation="MDTVSNKAKRPRKTTRPHIPGPARERGELHALVTAAADAFTATA
                     KGAPLLEHVTPEAFPFAVAVALEMLWRKFPGRRVWVFCTNARTQDAIHAGLTIWQTPA
                     LYYPRLGQSVADALPDPDLVAERVAVLSRWHEQAEDTPRALVLCADSLDEEAPDARVL
                     ASERRPLEVGATLDMEHFLTDLDKAGYERVPVVTERGQFSRRGGIVDFFSWQAEEPLR
                     VEWFEDQIESIRAFDLHNQASIRRLERASVVLQLDDEAATGKVRDYLGKDDIILAVEC
                     VLDMPHHARVASHPETVDAPEDFSTAIHENPLGVFDASDFVLHEARRKQLALQVREWQ
                     GLGWHVVIVFHNDAERERYAELVGRTGISPVSGETGILPASEKTGQNARSTRDGLEAH
                     PTLLLGLLYRGFTVPGAKLAVLSGAEIFGRHQQVRRVRGSKLDDPELLRKARDIMREL
                     RAGDIVVHSEHGIARYGGLEERTAGGRTEEVLALHYAEGARLFVPVAQAHLVSRYVGI
                     GGKTPSLSKLGGASWQKTRKTAEKSVEEFAARMLTISAERQSIQGYAHPPDTKWQIEF
                     EDSFLYHETPDQLRCVEEIKRDMESPKPMDRLLCADVGFGKTEVAIRAAFKAVMGGKQ
                     AAILVPTTVLARQHWETFRQRVSEFPVTVEMLCRLTPKKREKDILSGVRDGTVDIVVG
                     THRVISKDVRFKDLGLVVIDEEQRFGVRHKERFKELFRLVDVLTLSATPIPRTLYLGL
                     MGMRDMSTIETPPPSKQAVQTLICPYDERVIRQAVDAEIERGGQVFFLHNRVMTIEKT
                     AQRIRELCPRARVIVGHGQMDEELLEDVMHTFVDGKADVLVCTTIIESGVDIPNANTI
                     LIDRADRFGLADLYQLRGRVGRGGTQAHAYLMLPRESVTTGDARKRVNAIKQYTGLGS
                     GFKIALRDLEIRGAGNLLGTEQSGHIAAVGFDMYCQMLKQSVARMQGRRVPRPVEVAL
                     RADFLIQSEAHMSGALPDHATPAFLPAAFIEDTHLRITAYRQLGEVMTRRELDDLEAQ
                     WRDQFGQQLPHAVQNLLTCAALRLAAAHAGISTIEIKERKLMLTRHGQLIMPGGKFPR
                     LTERQGHKQLAEALNMLRSL"
     gene            10936..13509
                     /locus_tag="PLP58_11195"
     CDS             10936..13509
                     /locus_tag="PLP58_11195"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HRJ09128.1"
                     /translation="MSLPEIAGHELQDLIGSGRCGAVYRASAGGKACAVKVFSSMAIN
                     RKALGAALQALKQLPHHGGVLTVEHFNFDRSPYYAAMPLVGSMSRDAQGRRQWRTRTL
                     EDLFGGCDPDRAWNVIYQIADALSWLHKHGIPHGNLRPCNILLEDDAETSIRLTDIAQ
                     GWVGGIHHLELTDHFVHLCPEQAENPDGVFAGYGPGWDVYAFGVIAWRLLTGSLPRGG
                     KAWAAQVETVNQKAAAGLACGIDSAALLAAVRAQPRVVWPDAPSSKWDERRRHIIERC
                     LDLNSSARWSDMREVVREFEVLESDFLLEESREQTVQERKKQASKVLVLQTTVLGLFT
                     AFVLSTAYAFLTLRRARDAETVISQKDAILEQETGARDARIVELTGERDAALKAKATA
                     DANLRHAQGGVDKFLTQLLQTPTGNELEVEFSKTQLNEALDFCNASLPALEADPALGV
                     ERLRAYGNIGRIHLQLRQHTQALAFLQKAREQSAALLKEQPSSPQAALHHQWFGRYSL
                     LLSDLQQRRGDRALSLSLLQDATRHLTEGLAADPKNRLALNECARAWMEYGLRNMQNG
                     DLAEAGKALSRVGDVLDPKVLGDKPLPDESFLLARAKFAKGLVQRDSGHTQDALDTLI
                     EAVTEMGRLVMGSSPRNQEQALLLSEAYTELSELIGKTIGSKEAREAHDQAVPILLEL
                     NRLHPAWGDVKYLLARNYGAISLLERDLGNNNDALKKKQDAIELINEVLADEPDNLRY
                     AALQARLRGEYAELMSDLGKPTAALPILTQAVESLEGLLGQDPGARLTPERKEQEIQL
                     AQMHGVLGHTLQTLKKTSEAKTAFTSAVQRWEKLSALIPGDDIIQQGLTWSKERLGKL
                     K"
     gene            13610..15193
                     /locus_tag="PLP58_11200"
     CDS             13610..15193
                     /locus_tag="PLP58_11200"
                     /inference="COORDINATES: protein motif:HMM:TIGR00813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter"
                     /protein_id="HRJ09129.1"
                     /translation="MPYLLDALVILGYFALIIGIGLSQRSKSGSVEGFTLGDRQIAWW
                     AVLASILAAEISAATFLGAPESGYSRQNWSYAQFAIGTVLARIIVSFLFIPVFYRHGV
                     ISLYEYLETRFGPATRKFASITFMFTRVLAMGTRLYVSAIILVLAVAMWQGGAVDADT
                     KFWLYAGAVVVVTLLTALYTSVGGIRAVIWTDFIQVGVLVASLGFTIPYLLMKIPGGW
                     GAVAEVVKSPVFFDFAKPAGAGAWEWIKNVFTSEYTIWAAVIGSTFVTMSTHGIDQDT
                     VQRMLTAKNRRQSALATILSGIVDLPIVSAFILIGVLLKAYYAAHPAVNLPAESREVF
                     PFFIMNEMPAGMRGLVTAGILATAMGSLSTALNALATSLSRDFILPRLPADAPESKRI
                     AVLRWSTVLFAVLIIGVGVWTAWYMAHNPKVEILPLVLGILGFTFGSLLGVFLLAVLT
                     KTRGCDSGNIIAMLCGIAAVLFLSNVLGIQSRLGIDKPFVLSFPWRITLGTFVTLAVS
                     VMFPTPHNKRHNHPAADAD"
     gene            complement(15209..16081)
                     /locus_tag="PLP58_11205"
     CDS             complement(15209..16081)
                     /locus_tag="PLP58_11205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007416735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S1-like domain-containing RNA-binding protein"
                     /protein_id="HRJ09130.1"
                     /translation="MAQLGKHNQLKIVRGSMHGVYLDGGELGEILLPMKFVPQGAALD
                     DTLEVFIYRDSEDRLVATTERPLAMAGDFAALEVVAVNPQVGAFLNWGLAKDLLLPFR
                     EQEGRARPGEKVVVHVLVDPKSDRIIASGRLARHASRQPAPWRTGSRVELLIAGKTPL
                     GWNAVIENSHLGLLFTQPGDPPPHPGLRVQGYVTSVRPGGKIDLSLNAAGYQRVAPLG
                     DRILQALKANGGRLDLDDDSPPEVIRESFGASKKAFKQALGALFKQKRIRFTRPGIEW
                     VDITRPREGDWRPR"
     gene            complement(16092..16772)
                     /locus_tag="PLP58_11210"
     CDS             complement(16092..16772)
                     /locus_tag="PLP58_11210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009962253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRJ09131.1"
                     /translation="MPLISLSEIHKTYREPGGGELRVLRGISQEIEAGETVAVVGPSG
                     CGKSTLLNIIGTLDTPDSGSYVLDGEEVSRCAPERLAALRGGKIGFIFQLHHLMPQCT
                     VLENVLLPTLALKDRPDSKAARSRGESLLASVGLAGRLDWKPAQLSGGERQRVAVVRA
                     LINAPKLILADEPTGALDEKNAESLTGLLLELCQSTGATLVMVTHHPAQAARMSRTLR
                     LHEGRLAS"
     gene            complement(16823..>17513)
                     /locus_tag="PLP58_11215"
     CDS             complement(16823..>17513)
                     /locus_tag="PLP58_11215"
                     /inference="COORDINATES: protein motif:HMM:NF023410.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="exosortase-associated EpsI family protein"
                     /protein_id="HRJ09132.1"
                     /translation="AHVAALALAAGGGMAACVLTDTGYVVAPPGIGMDLPGRHAGYES
                     VDMPMNTLERQILHEDVTIGRRFYMTPQRAILASVVMSGVIKRSLHEPQICLPGQGWM
                     IDGKEYIEVDAGLERPVKATVLSMHRDTQGADGGVKRTRALNVYWYFGSDGTTAASYD
                     EHVAVSYFDSVLKNLNHRWALVSFFAPMREQMLGMDDPFAELAVLEDVRTFIRGLVPE
                     LVAQAGRVAPP"
BASE COUNT         3057 a         5972 c         5489 g         2995 t
ORIGIN      
        1 ctggcgctgg ggttggtggt ggtgggcgtg cagacgggca tcggcgcggc gcgcgtggcg
       61 ccgccggatt tgcggctgtt cgggatgtcg ccaaaggagt ttctggtggt gccgctgggg
      121 agcatcatga tgtttggcgc gctggtctgc gcgggtgtgg cggcgcggcg gcgtccggcg
      181 gcgcaccgcc cattgatgtt catggcctcg ctggccgcgg tgtccgcggc gctggggcgc
      241 atcgggccgc tcaacacttg gtatgccggg acctggcttg agcgggggtt cagcgcgttc
      301 ctgaccatgc tgctgctggg cgcgctggtg ctgggtgtga agtggggcgt gacccggcgc
      361 ttcgacgggt ggttcgcgtc cgtgctggcg gcgctggcgg tttgcagcat ggcgctttcc
      421 ctgggttcga agacgggagc gtgggacagc ttcgccacct tcctgctgcg gtgagaagtc
      481 cgaaaacaac acaccgtcat gcccgccgcc agcgaccgcc ctttccgcct gctctttgtc
      541 tgcatgggaa acatctgccg ctcgccggcg gcggagggtg tgatgcgcag gctgacgcgc
      601 gaggcgggcc tggcggagca ggtggagata gactcggcgg gcaccggcgg ctggcatgag
      661 ggcagcccgc cggatgacag gatgactgcg gcggcccgcc gccgcggcta tgagctgacg
      721 ggacgggcgc ggcagatcac ggcgggggac atccgcaggt ttgatcttgt gctgatcatg
      781 gacgagcaga accggcggga catccggccc tggctgccgg acgggccgga gcgggaaaag
      841 gtgcgcatgt tctgcgagtt ctgcgcgcgc ttcgagaatg acgaggtgcc ggacccatat
      901 tatggcggca gggagggctt tgaccgcgtg ctggacctgc tggaggacgg ctgcgcgggc
      961 gtgctggagc acatccggca gtggcgcgcg ggcgcggctg aaaagccgtg agcaaagcgc
     1021 gcggatttgg gattgcaaat agcgccagcc tgcctctaaa cgtggcgcat gactttattg
     1081 ctcgccgagg tggagaccgc gcaaatgacc ggccaatgga tcggctccgt gcttggcatt
     1141 ttgctgcttt cggccggttt gatcaaatgc ctgactttgc tgcgccgtcc ggcgacgagc
     1201 aagacatgcg tgtcggcgct ggcgctggct ttggctggct ggctgggcgc cctgttgttc
     1261 caatccatcg cgttccttgt gccggaggcg ctggcggtgg tttatgtgat cggcgggctg
     1321 ggcatggccg gcctgatgct ggcggccatc gtcacaggca tcactggtct ggcgctttat
     1381 gactcgaagg ttcacacgca gggccgcgcg caggcggtgt ggagcctggt gatcgccgtg
     1441 ttgatgctga cggtggctgt cacgggcctt gttttgcaaa tgcggctcga aagcgcggca
     1501 aacaagctgg cttccctctc aggcaagggc gccgagatca ggagcgcgga gttcaactgc
     1561 tccctggaaa tcccgccgcg ctggctggag acgaagccgg cgctgatcaa caagctggcc
     1621 tgcatcgcgg cgcggcgcgc ggcgccggag gcttatgtga tggtgctggg ggagcacatt
     1681 gacggcgaga tggatctgga ggcgctgctc gacgcggtga aggcgaacat ggccgccacc
     1741 tcgaaaagcg tgaccggtga aacggaggag tctctcaccg tggatggcgt gcctttcatg
     1801 cgccgcgcct gcctggtgga gaatgacgcg gccaaaggca tcaagctcta cttcgagcag
     1861 tgggtgtcca cactgcccgg ctacgcctgg cagttgcaca tgtgggggcc ggtggcgggc
     1921 agggcggatc tggagcctca gttcaagcgc atcggcgaaa gcttccgcgt gctggacaag
     1981 cagcgcgtgg tgccggccag cgcggccctc gacaatgtca gccgcccgca gtggggctac
     2041 cgcaccgagt tcaacgcggg cgactggaag aagtggggcg tcgagggcca cgagcagact
     2101 ctggccgact tcgcagcgct gcgcccgctg gaggcgttca tcgtgctgcc ggtgaacctg
     2161 gggccggagc ctccgccgct ggaggccgcc gcctccggcc tgctggaccg gttcggcatt
     2221 ccctttccct ccgatgactg gccggcgcga aaatggccga acgcctgggg cgagggcatc
     2281 gagatcaccg gcgtgcgctc gctggaggac ggggactaca gttacagaat gcgggtggcg
     2341 gcgcgcgggc cggtggcctt gctcatcgcg ggctggcacg cggtgaagaa cggcgatccc
     2401 gaaatggtgc gcaaagcggt ggatggcgtg gtgctgctgc ccggagagcc gggggagatt
     2461 cccggcctgg aggaaagcca gcgcggtcaa tacggcctcg tgtgcaacga catcggcatc
     2521 tggcatcacc agcgccagca gaccggcaag gcggcggagt ggttttggcg cggctttcag
     2581 cagactcaaa aagacccgac catcctgggc aacgcgctgg atgcctggca gcagacgggc
     2641 cgggccgccg aggcgctgga gaaggctggg gagcacatca agaaattccc cgcgcatgtg
     2701 aacacgcagc tcagccacgc ccgcctgctc tgcgatcttg gccgcgatga cgaggcggca
     2761 gatgctttcg ccgccgccct caaggccggt gtcaaagatc ccgacgccgt tttgcgctgg
     2821 ctgcaatacc tcaacggcgc ggaaaaatac gccctggccg aaaagaccgc cgcggcctgg
     2881 atggagcaaa gcggcaacct cgactcccgg cgctggcacg cccagaccgt cgccagcaac
     2941 ggcggcaagg cgcgcgcgat ccagttgttc gaaaaaatgg tggaggagca tcccgaggac
     3001 cggcgtctgc tttacgaact ggcggagacg cgcaatgacg cggcggaata cctccaggcc
     3061 gccgaactgg cgcagggcat cctcaaagac acgccggaca gcgcgcgcgc ctggatgatc
     3121 ctgggctgga gccagatggg gaggaagtgg taccgcgagg ccaagaaatc ctttgaagaa
     3181 gccctggaga agcagcctga cagcgaggaa atccagcagg cgctgcgccg cgcctctgcc
     3241 atgctcggcc agggcagcaa ctccgacatc aagaccccca tcgagccggt ggcgctgccg
     3301 gaggaattgc gggagctcct ggagaaaaat ccgcccgccg ccggccatgc cgcagggcag
     3361 ccggtggcat ggctgctttc ggccacaggc taccattttg aaaaggacaa accgctgcgc
     3421 accacatggc atgtgcgcgt gaagctgcgc gacagcgagg gcgtgtcccg cttcagcagc
     3481 gtcgagcaca actttgaccc gctgggcagc cgcgttttca tcaacagcgt cgaagtgcgc
     3541 gacgaggagg gcagggtcgt cgccagcgcc gccggagacg cctatgtgat ggatgtggac
     3601 aacggcatgg gcacccatga caaaaagctg cactttcaaa tacccggcct gcgcccgggc
     3661 tgcgtggtcg agtatgtgat cagcaaggaa agccgctaca aggcggagac cttcgagttc
     3721 aaccggcacc tgttcagcga ctccgccgcc gacatcgtgt ttgtcaccgg tgacgtggac
     3781 ggcgtcgcct tcgcgggcaa tgccgcgctg gcggatctca agcatctgcg ccaggaccgg
     3841 ctgctggcat ggaccggcct gcacctgccg cagagcacgc gcgagccttt ccccgtcctc
     3901 atggaggagc gcgtgccctg cctggcgctc ggaggtcatg agggaagctg ggcggaggtc
     3961 ggcgacaaac tgctcgacaa ggaactcgcc gggcggctcg aaccggaggc tgaagtcaca
     4021 gcactcgcac aaaggctcat tcagggtgtg ggaagcgaag gcgaaaaaat cgccgcgctc
     4081 gccaggcatg tgcagcaggc catcagctac accgccatcg agttcggcac gcgcgcgcgc
     4141 cgcccgaatc ccgccgcgca gacgctcggg cagaagtatg gcgactgcaa agaccaggcg
     4201 ctgctgctgc accagctcct caccgccgcc ggcatcggca gccacctcgc gttggtgagc
     4261 aacaactggc gcacgcgggt cgaacttccc tctgtggatc agttcaacca catggttgtg
     4321 catgtgcccg cgctcggccc cggcttcctc attgacacca cggacaaaaa tctcgccctc
     4381 gtcagcctgc acgcggacaa tctctggcac agccacgcgc tggtgcttga gcgggggaag
     4441 tcccgcctca tcgagccggc gcagcccgcc cccggcagcg cgcgcatcct cagcgagcgc
     4501 aaggtgcgcc cctccggaga cggctgggag gtcaatgaaa gcctcgtcat tcacggctac
     4561 tacgcggcat ggatgcgcgg cgccttcacc ggcaaggacg cccaaaggca gttgcagaac
     4621 gtgcagaaca tcctcgcagc ccacgcgccg gccctgcgcg tgcatgagtt cacctttcat
     4681 catctcgaag acaccggcct gcccgcccgg ctggagatcg cctacgaagt gcccggccgc
     4741 ctccaggatg agggggcgct gcggcgcggc gcgctgcccg cgctgtggga gcaggagtac
     4801 ctgggcacca cttttgtgaa gaaccgcgcc agcgccttcc gcttcatcta tccgctccag
     4861 ttcgagtccg atctgcgcgt cgaaggcgcg ggcggcattc cagaggacgc gctcaaggcg
     4921 ctcaacgtct cgcgcgaagg ccgcttcgcc tcctggaaaa tggaggctgc cgcgcagggc
     4981 gggggactcc tcaaactgcg cttccgcttc tccacccagc ccggcaccta tgacgccgcc
     5041 gactacccgg actggcacga gcagtggaac agcgccgtga gtcagtggca gcgccccttt
     5101 tcctggaaac cctgacgcgc cgcctccgca ctgcaatgat gccgcccgcg cgcgcgtttg
     5161 gcctggatgc aatgcgacgg cgtcattctc ggatcagggc acaacgcgct cgtgctcgcc
     5221 tgctacgcgg cgcgcgccgg catgagcatc gtcgtggtcg agaaaaatga cgaggccggc
     5281 ggcggtctcg ccaccgtgga gcatccggcg gggtccggct tcatgcacaa cacccactcc
     5341 ttcttccacc gcgccatcac caccatgccg tggtatcagg acctcggact tgaacggcac
     5401 ggcgcgcgct acatcgagcc ggacctcaat gtggcgatga tcctgccgga cgagcgcgtg
     5461 ctggagtggt ggacggactt tgaaaagact ttcgcctcct tcgctgccat ctccccgcgc
     5521 gatgccgccc gcctgcgcga gtggacggaa cgcttccgcc ccatcgtcac agacatcctc
     5581 atcccggagg cgcaaaaccc gccgctgcct atggaggagc gcctgtcaaa gctgagtcaa
     5641 agccaggccg ggcgcctgct gctggagacg caggccctgt cgccgattga gttcgtcatg
     5701 cgcgagttcg agcatcccgc cgtgcgcgcg ggactgcttt ttttcaacgg cctgcgggag
     5761 gtggacctgc gccagaaagg cttcggacac tccatccccg cgctgctcgc cagcccgcgc
     5821 aaggcgcaga tgtgcgccgg cggctcgcgc agacttgcgg aggcgctgtg cgcggaactt
     5881 cgcgggcatg gcggccggat tctgaccggc tggcagccgc gttccatcct gatgcgagga
     5941 ggacgcgcct gcggtgtcga gtccttgacc ggcgagcgcg tcgaagcggc gcgatttgtg
     6001 gccagcggac tgaacccgca gcagaccttc attgatctca tccccgccga cgccgctcct
     6061 tcgagcctgc gcgagcgcgc cgccggcttc gagtacaacc tgctggcgcc gctcttcgcc
     6121 ctgaacctca acctgcgcga gccgcccgcc tacacagcgg cggagcggca tcccgagttg
     6181 agccgcgcat tcatggtcat ccttggcctg ggtgatgtga cgcagttcca ggaaatcgtc
     6241 tccgcccacg agcgcggcga aatcccgcgc accgtcatgt ggggcgcgtg cccgactttg
     6301 ttcgacccct cgcaggcccc gcccggcagg cacaccgcct tcatgtggga aaagctgccc
     6361 tatgccctgc gcggtgacgc gcgaaattgg gacgcctgca aggaagcgca tggccgcgtg
     6421 atgctcgacc tgtgggcgaa acacgcgccc aatcttcatg acgccgtcct cggcagcttc
     6481 acccgcagcg cgctcgacac cgagcggctg ctgccaaaca tgatgcgcgg cgatctgctc
     6541 gtgggctcct ttgccaacgg ccagacgggt tttcaccgcc ccttcaaagg cgcgggcagc
     6601 taccgcaccg aaatccccgg cctctatctc tgcggcggct ccacgcatcc cggtggcaat
     6661 gtcaccggcc tctgcggctt caatgccgcc agagtcctgc tagccgacct tggggtcgag
     6721 cgaggcacgc tttctgtggt cccgccgtga tgccttccgg catgtccggc tagggcgtcc
     6781 cgctgccggc aagactgaca agcatcaccg catgctgctc atacaccgcg cgggcgccgg
     6841 cgacgcgggc gatgtattcg atggcctcgg tgagcgggat gttgttgagt tgcaggctca
     6901 gtgtcttcgc cttcaactcg gcatccttga tgatgatgtt tggcatcact ttgccttcgg
     6961 tggaggtttt gcacaggccg cgcaggcctt ccaccgcttc ttccaggctg acatccacaa
     7021 actcgatctt cgccagcgtc acgcccgagt aagttttctt caacgaaccg gtgccggtct
     7081 tcatgtgagt gcggatttgg gccagcaaaa ccttggtttc atagtggttc ggctgcttcg
     7141 cctccacctg cttgaggagc tggtaggcca tctccatgtc gcctttgtag taggccgcgc
     7201 gtcccatttg aaacaaggct tgcaggtcgt cattcgctcc ggcttccggc attcctacgg
     7261 tcatcaagac aaccgccgca ctgatcaccc atcccttgaa tcgtgttttc atgattttgt
     7321 gagtttggtt ttcgggagca tttcatcaaa aatccgccgg aagcgcaaga tttgttttca
     7381 cggaaataat catcgcggct ttgcggcggc tgggcacgtt cttaccaccg ccctttggac
     7441 acggtttcga acaaggccaa acgcccccgc aagaccaccc gcccgcacat tcccggcccc
     7501 gcccgcgaac gtggggaatt gcacgccctc gtcacagccg cggcggatgc tttcaccgcc
     7561 acggcgaagg gcgcgccctt gctggagcat gtgacgccgg aggcgtttcc ctttgccgtg
     7621 gccgtggctt tggaaatgct ctggcgcaag tttcccggca ggcgcgtctg ggtcttctgc
     7681 accaatgccc gcacgcagga tgccatccac gcgggactca ccatctggca gacgcccgcg
     7741 ctgtattacc cgcgtctcgg gcaaagcgtg gccgatgcgc tgccggaccc cgatctcgtg
     7801 gccgagcgcg tggccgtgct ctcgcgctgg catgagcagg ctgaggacac accgcgcgcg
     7861 ctggtgcttt gcgccgacag tctggatgag gaagcgccgg acgcgcgtgt gcttgcctcc
     7921 gagcgcaggc cgttggaggt cggcgccacg ctcgacatgg agcacttcct caccgacctc
     7981 gacaaggccg gctacgagcg cgtgcccgtc gtcacggagc gcggccagtt ctcgcgccgg
     8041 ggcggcattg tggacttctt ttcctggcag gccgaggagc cgctgcgcgt ggagtggttc
     8101 gaggatcaga tcgagtccat ccgcgccttt gacctgcaca accaggcctc catccgccgc
     8161 ctggagcgcg ccagcgtcgt gctccagctc gatgatgaag ccgccactgg caaagtgcgc
     8221 gactacctcg gcaaggatga catcatcctc gccgtcgaat gcgtgctcga catgccgcac
     8281 cacgcccgtg tcgccagtca cccggaaacg gtggacgcgc ccgaggactt cagcaccgcc
     8341 attcatgaaa atcccctcgg cgtcttcgac gcgtccgact tcgtcctgca cgaggcgcgg
     8401 cgcaagcagc tcgccctcca ggtgcgcgag tggcaggggc tgggctggca cgtcgtcatt
     8461 gtctttcaca atgacgccga gcgcgagcgg tacgcggagc tggtgggtag gacaggcatt
     8521 tcgcctgtct caggtgaaac gggcattctg cccgcctctg aaaaaaccgg gcagaatgcc
     8581 cggtccaccc gcgacgggct ggaagcccat cccactctcc tgctcggcct gctctaccgc
     8641 ggcttcaccg tgccgggggc aaagctcgcc gtgctcagcg gcgcggagat cttcggcagg
     8701 caccagcagg tgcgccgcgt gcgcggatcg aagctcgacg atcccgaact gctccgcaaa
     8761 gcgcgcgaca tcatgcgcga gctgcgcgcg ggcgacatcg tcgtgcatag cgagcacggc
     8821 atcgcccgct acggcggcct ggaggagcgc accgctggcg gacgcaccga ggaggtgctc
     8881 gcgctgcatt atgccgaggg ggccaggctc ttcgtgcccg tggcccaggc gcacctcgtc
     8941 agccgttacg tcggcatcgg cggcaagacg ccctcgctca gcaagctcgg cggcgcctcc
     9001 tggcagaaga cgcgcaagac cgccgagaaa agcgtcgagg aattcgccgc gcgcatgctc
     9061 accatcagcg ccgagcgcca gagcatccag ggttatgccc atccgcccga caccaaatgg
     9121 cagatcgagt tcgaggactc cttcctctac cacgagacgc ccgaccagct ccgctgcgtg
     9181 gaggagatca agcgcgacat ggagtcccca aaacccatgg accgcctgct ctgcgccgat
     9241 gtcggcttcg gcaagaccga ggtggccatc cgcgccgcct tcaaagccgt catgggcggc
     9301 aagcaggccg ccatcctcgt gcccaccacc gttcttgccc gccagcattg ggagaccttc
     9361 cgccagcgcg tgagtgaatt ccccgtcacc gtcgaaatgc tctgccgcct caccccgaag
     9421 aagcgggaaa aggacatcct gagcggcgtg cgcgacggca ccgtggacat tgtcgtcggc
     9481 acccaccgcg tcatctccaa agacgtgcgc ttcaaagatc tcggcctcgt cgtcatagat
     9541 gaggagcagc gcttcggcgt gcggcacaag gagcgcttca aggagctctt ccgcctcgtt
     9601 gacgtgctca ccctcagcgc cacgcccatc ccgcgcaccc tgtatctcgg cctcatgggc
     9661 atgagggaca tgagcaccat tgagaccccg ccgcccagca agcaggccgt gcagaccctc
     9721 atctgcccct acgacgagcg cgtcatccgc caggccgtgg acgccgagat cgagcgcggc
     9781 ggccaggtct tcttcctgca caaccgcgtc atgaccatcg aaaagaccgc gcagcgcatc
     9841 cgcgagctat gcccgcgcgc ccgggtcatc gtgggccacg gccagatgga tgaggaactg
     9901 ctggaggacg tcatgcacac cttcgtggac ggcaaagccg acgtcctcgt ctgcaccacc
     9961 atcatcgaaa gcggcgtgga catccccaat gccaacacca tcctcattga ccgcgccgac
    10021 cgcttcggcc tggccgacct ctaccagctc cgcgggcgcg tcggtcgcgg cggcacccag
    10081 gcccacgcct acctcatgct cccgcgcgag tctgtcacca ccggcgacgc gcgcaagcgc
    10141 gtcaatgcca tcaagcaata caccggcctc ggcagcggct tcaagatcgc cctgcgcgac
    10201 ctggagatcc gcggcgcggg caacctcctc ggcaccgagc agagcggcca catcgccgcc
    10261 gtcggcttcg acatgtactg ccagatgctg aagcaaagtg tcgccagaat gcagggccgc
    10321 cgcgtcccgc gccctgtcga ggtggccctg cgcgccgatt tcctcatcca gagtgaggcg
    10381 cacatgtccg gcgccctgcc cgaccacgcc acccccgcct tcctccccgc cgccttcatc
    10441 gaggacaccc atctgcgcat caccgcctat cgccagctcg gcgaagtcat gacccgccgc
    10501 gagcttgacg atcttgaagc ccagtggcgg gaccaatttg gccagcagct ccctcatgcg
    10561 gtgcaaaacc tgctcacctg cgccgccctc cgtctcgccg ccgcgcacgc cggcattagc
    10621 accatcgaga tcaaagagcg caagcttatg ctcacccgtc acggccagct catcatgccc
    10681 ggcggcaaat ttccccgcct cactgaacgc cagggccaca aacagctcgc cgaagccctg
    10741 aacatgctgc gcagcttgtg agggatggct ttgaaccggc ctgtgccgct caacacgccc
    10801 ggcacccagc cggtttgaag ccgccatcct gccaagcgcg ccttgacccc gcgaggcacg
    10861 cgatgcacta tggcaggccg tgactgacgc ggcatccccc tcccccaacc cgatgacaaa
    10921 acccgcgcgc aaagcatgag tctgccggag atcgcaggac acgaattgca ggacctgatc
    10981 gggagcggca ggtgcggggc cgtctatcgc gccagcgcgg gtggcaaggc ctgcgccgtg
    11041 aaggtctttt cctccatggc catcaaccgc aaggcgctgg gcgcggcctt gcaggctctc
    11101 aagcaattgc cccaccacgg cggcgtgctg acggtggagc acttcaattt cgaccgcagc
    11161 ccctattacg ccgccatgcc gctcgtcggc tccatgagca gggacgccca gggccggcgc
    11221 caatggcgga cacgcacgct ggaggacctg ttcggcggtt gcgaccccga tcgcgcttgg
    11281 aacgtcatct accagatcgc cgacgcgctc tcctggctgc acaagcacgg catcccgcac
    11341 ggcaatctcc gcccctgcaa catcctcctc gaagatgacg ccgagacgag catccgcctc
    11401 accgacatcg ctcagggctg ggtcggcggc attcatcacc tggagctgac cgaccacttt
    11461 gtccacctct gcccggagca ggcggaaaat cccgacggcg tttttgccgg ctacggaccc
    11521 gggtgggatg tctatgcctt cggcgtcatc gcctggcgtc tgctcaccgg cagcctgccg
    11581 cgcggcggca aggcctgggc cgcccaggtg gagactgtga atcaaaaggc tgccgccggg
    11641 cttgcctgcg gcatagacag cgccgcgctg ctggccgccg tccgcgccca gcccagggtc
    11701 gtttggccgg acgcgccctc gtccaaatgg gacgagcgcc gccgccacat cattgagcgc
    11761 tgcctcgatc ttaactcctc cgcgcgctgg tccgacatgc gcgaggtggt gcgggaattc
    11821 gaagtgctcg agtcggattt cctgctggaa gaatcgcgcg agcagaccgt gcaggagcgc
    11881 aaaaaacagg cgagcaaggt gctcgtcctg cagaccacgg tgctgggcct gttcaccgcc
    11941 ttcgtgctca gcacggctta cgctttcctc acgctgcgcc gggcgcggga cgcggagacg
    12001 gtcatcagcc agaaagacgc catcctggag caggagacag gggcgcggga cgcgcgcatc
    12061 gtggagctga caggggagag ggatgcggcg ctgaaggcca aggccaccgc cgacgccaac
    12121 ctccgccatg cccagggcgg cgtggacaag ttcctcaccc agcttctgca aactcccacc
    12181 ggcaacgaac tcgaggtcga attcagcaag acccagctca acgaagccct cgatttctgc
    12241 aatgcctcgc ttccggcttt ggaggctgat cccgccctcg gcgtcgagcg cctgcgcgcc
    12301 tatggaaaca tcgggcgcat ccatctccag ctccgccagc acacccaggc cctcgccttc
    12361 ctccagaaag cccgcgagca aagcgccgcc ctgctcaagg agcaaccctc ctccccgcag
    12421 gccgcgctgc atcatcaatg gttcggccgc tacagcctgc tgctctcgga ccttcagcag
    12481 cgcaggggcg accgcgccct ctccctcagc cttctccagg atgccacacg ccatctcacc
    12541 gaaggcctcg ccgccgatcc caaaaaccgc ctcgcgctca atgagtgcgc ccgcgcctgg
    12601 atggaatacg gcctgcgcaa catgcaaaac ggcgacctcg ccgaagccgg caaagccctc
    12661 tcccgggtcg gcgatgtgct cgaccccaaa gtgctcggcg acaagcctct gcccgacgag
    12721 agcttcctgc tcgcccgcgc caagtttgcc aagggactcg ttcagcgcga ctccggccac
    12781 acccaggacg cgctcgacac cctcatcgaa gccgtcacgg aaatgggccg cctggtcatg
    12841 ggcagctccc ctcgcaacca ggagcaggcc ctgcttctct ccgaggccta caccgagctc
    12901 tccgaactca tcggcaaaac catcggcagc aaggaagccc gcgaggcgca tgaccaggcc
    12961 gtgcccatcc tgctcgaact caaccgcctg catcccgcct ggggggatgt gaaatacctc
    13021 ctggcccgca actacggcgc catctcactg cttgagcgcg acctcggcaa caacaatgac
    13081 gcgctcaaga aaaagcagga cgccatcgaa ctcatcaatg aagtcctggc tgacgagccg
    13141 gacaatctcc gctacgcggc tcttcaggcg cgcctgcgcg gagaatacgc cgaactcatg
    13201 tccgacctcg gcaagcccac cgccgccctg cccatcctca cccaggctgt ggagtcgctg
    13261 gagggactgc ttggccagga ccctggcgcg cgcctcaccc cggagcgcaa ggagcaggaa
    13321 atccagctcg cccagatgca cggcgtgctt ggtcacaccc tgcaaaccct gaaaaaaacc
    13381 tccgaggcca aaaccgcctt caccagcgcc gtccagcgct gggaaaaact ctccgccctc
    13441 atccccggag acgacatcat ccagcagggc ctgacgtggt ccaaggagcg acttggaaaa
    13501 ctgaagtgac ccgcgccagc agagtgcctg ccagccggct tgacggaacg gtgcgcggca
    13561 tggtcaggtt gtcctggcaa cgtgggaatg tccattccat actcgcgcca tgccctacct
    13621 tctggacgcc ctcgtcattc ttggctactt cgcgctgatc atcggcatcg gcttgtccca
    13681 acgcagcaaa agcggaagcg tggagggttt cacgctgggt gaccgccaga tcgcctggtg
    13741 ggccgtgctg gcatccattc tggcggcgga aatcagtgcg gcgacatttc ttggcgcgcc
    13801 ggagtccggc tacagcaggc aaaactggag ctacgcgcag tttgccatcg gcaccgtgct
    13861 ggcgcggatc atcgtgagct ttctgttcat ccccgttttc taccgccatg gagtcatctc
    13921 gctttacgag tatctggaga cccggttcgg gccggcgacg cggaagtttg cctcgatcac
    13981 gttcatgttc acccgcgttc tggcgatggg cacgcgtctc tatgtctccg ccatcatcct
    14041 cgtgcttgcg gtggccatgt ggcagggcgg cgcggtggat gcggacacga aattctggct
    14101 ctatgcgggg gcagtggtgg tggtgacatt gttgaccgcg ctctacacct ccgtcggcgg
    14161 catccgcgcg gtgatatgga cggatttcat tcaggtcggt gtgctggtcg cttctcttgg
    14221 attcaccatc ccgtatcttc tgatgaagat tcccggcggc tggggcgcgg tggcggaggt
    14281 ggtcaaatca cccgtgttct tcgacttcgc gaaacctgcg ggagcgggtg cctgggagtg
    14341 gattaaaaat gtgttcacca gcgagtacac gatctgggcc gcagtcatcg gcagcacgtt
    14401 tgtgaccatg agcacgcatg ggatagacca ggacaccgtg cagcgcatgc tcacggcaaa
    14461 aaaccgccgc cagagcgccc ttgccaccat tctctccggc atagtggacc tgcccatcgt
    14521 ctccgctttc atcctcatcg gcgtcctgct gaaagcctat tacgcggcgc atccagccgt
    14581 gaacctcccc gcagagagcc gcgaggtttt ccccttcttc atcatgaacg agatgcccgc
    14641 cggcatgcgc ggtcttgtca cggccggcat cctcgccaca gcgatgggtt ccctcagcac
    14701 cgcgctcaat gccctcgcca caagcctctc gcgcgacttt atcctgccgc gccttcccgc
    14761 tgacgccccc gagtcaaagc gcatcgccgt gctgcgctgg agcaccgtgt tattcgcggt
    14821 gttgatcatc ggcgtcggcg tctggaccgc ctggtacatg gcgcacaatc ccaaggtgga
    14881 aatacttccc ctcgtgcttg gcattctcgg cttcaccttt gggtcgctgc tcggcgtgtt
    14941 tctgctcgcc gtgctcacca agacacgcgg gtgcgattcc gggaacatca tcgccatgct
    15001 gtgcggcatc gcagccgtgc tgttcctcag caacgtgctc ggcatccagt cgaggctggg
    15061 cattgacaag ccctttgtgc tgtccttccc gtggcgcatc actttgggga cattcgtcac
    15121 tctggcggtt tcagtcatgt tccccacccc tcacaataaa cgccacaacc atccggcggc
    15181 ggacgctgac tgagcatcgc gccccccctc accgcggacg ccagtcacct tccctgggcc
    15241 gtgtgatgtc cacccattcg attcccggcc gggtgaagcg gatgcgcttt tgtttgaaaa
    15301 gcgcgccgag ggcttgtttg aaggcttttt tgctggcgcc gaaactctca cggatcactt
    15361 ccggcggcga gtcatcatcc aggtcaagac gccctccatt ggctttgagc gcctgcaaaa
    15421 tccggtcccc caaaggcgcc accctctggt aaccggcggc gttcagactg aggtctattt
    15481 tccctccggg gcgcaccgat gtcacatagc cttgcacacg caggccggga tgcggcggcg
    15541 gatcacccgg ctgggtgaaa agcaggccga ggtgggagtt ttcaatgacc gcgttccagc
    15601 ccagcggtgt tttgcctgcg atgagcagct ccacccggct ccctgtgcgc cagggcgcgg
    15661 gctggcggct cgcatgacgc gccagtctgc cgctggcaat gatgcggtcg ctcttcgggt
    15721 ccaccagcac atgcaccacc actttttcgc caggccgcgc gcgcccctcc tgctcccgga
    15781 aaggcagcag caaatccttg gcaagccccc agttgagaaa ggcccccacc tggggattca
    15841 ccgccacgac ctccagcgcg gcaaaatccc ccgccatcgc cagcggccgc tccgtcgtgg
    15901 ccacgagccg gtcctcggag tcgcggtaaa tgaaaacctc cagcgtgtca tccagcgccg
    15961 cgccctgcgg cacaaacttc atcggcagca gaatctcgcc aagctcgcca ccgtcgagat
    16021 aaaccccgtg catggaaccc cgcacgattt tcagttggtt atgtttgcca agttgggcca
    16081 tgcgcagcct tctacgatgc cagtcttccc tcgtgcagcc gcaaagtccg cgacatgcgc
    16141 gccgcctggg cgggatggtg cgtgaccatg accagcgtgg cgcccgtgct ttggcagagc
    16201 tccagcagca gccccgtgag agactcggca ttcttttcat ccagcgcgcc ggtcggctcg
    16261 tcggcgagga tcagcttcgg cgcgttgatc agcgcgcgca ccacggccac gcgctggcgc
    16321 tcgccgccgg aaagctgggc cggcttccaa tccagacgcc cggcgagccc caccgaggcc
    16381 agcaggcttt ccccgcgtga gcgcgcggct ttggagtccg gacggtcttt cagagccagg
    16441 gtgggcagca gcacattctc cagcaccgtg cattgcggca tgagatggtg gagctggaaa
    16501 atgaagccga tcttcccgcc gcgcagcgcc gccagacgtt ccggcgcgca gcgggagacc
    16561 tcctcgccgt cgagcacata actgccggag tcgggagtgt cgagagttcc aatgatgttg
    16621 agcagcgtgc tcttgccgca gcccgaggga cccaccacgg ccaccgtctc cccggcttcg
    16681 atttcctggc tgatgccgcg cagcacgcgc agctcgccgc cgcccggttc gcggtaggtc
    16741 ttgtggattt cggaaaggga gatcagtggc atgtcagcag aaaggtgcgc ctgcgcggcg
    16801 gggcaagcgg aattcgtcgc gactatggcg gcgccacccg gcccgcttgc gccacaagct
    16861 ccggcaccag gccgcggatg aacgtcctca catcctccag caccgccagc tccgcaaacg
    16921 ggtcgtccat gccgagcatc tgctcgcgca tcggcgcgaa gaaggacacc agcgcccagc
    16981 ggtggttcag gtttttcaaa accgagtcga aatacgacac cgccacatgc tcgtcatacg
    17041 aggccgccgt cgtcccgtcc gagccgaagt accagtaaac attcagcgcc cgcgtgcgct
    17101 tcactccccc gtccgcgccc tgcgtgtcac ggtgcatgga gagcaccgtg gctttgactg
    17161 gccgctccag tcccgcgtcc acttcgatgt attccttccc gtcaatcatc catccctggc
    17221 ccggcaggca gatttgcggc tcgtgcagcg agcgcttgat gactccgctc atcaccaccg
    17281 atgccaggat cgcgcgctgc ggcgtcatat aaaagcgccg cccgatcgtc acatcctcgt
    17341 gcaggatctg cctctccagt gtgttcatcg gcatgtccac gctctcatac cccgcgtgcc
    17401 gtcccggcag atccatgccg atccccggcg gcgccaccac atagcccgtg tccgtcagca
    17461 cacaggccgc catgccgccg cccgccgcca gcgccagagc cgccacatgc gcc
//
LOCUS       DAOMYJ010000093        17471 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Prosthecobacter sp. isolate AMR_MDS_5629
            NODE_1122_length_17471_cov_19.262000, whole genome shotgun
            sequence.
ACCESSION   DAOMYJ010000093 DAOMYJ010000000
VERSION     DAOMYJ010000093.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867850
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prosthecobacter sp. (wastewater metagenome)
  ORGANISM  Prosthecobacter sp.
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Verrucomicrobiaceae; Prosthecobacter.
REFERENCE   1  (bases 1 to 17471)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17471)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.30
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:34:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,742
            CDSs (total)                      :: 4,684
            Genes (coding)                    :: 4,676
            CDSs (with protein)               :: 4,676
            Genes (RNA)                       :: 58
            tRNAs                             :: 55
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17471
                     /organism="Prosthecobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1122_length_17471_cov_19.262000"
                     /isolate="AMR_MDS_5629"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1965333"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..548)
                     /locus_tag="PLP58_11220"
     CDS             complement(<1..548)
                     /locus_tag="PLP58_11220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ09133.1"
                     /translation="MKPDFSAQSNFRWLKTEHHDLLFWGIKPPLPWPKEDDKKIDPQK
                     HFPFERLLEGIERLRQAGTGWSEEWDAVKTFIERGDELGEALESGDMTATLRLLDELE
                     TLRPGTPYCAFNKAFALKSLGDKEAALEAAKQATQREPKLEYLWMQRANLHEELGGTE
                     HEEKAAYCFRKALGLLPNHKQAL"
     gene            complement(626..3040)
                     /locus_tag="PLP58_11225"
     CDS             complement(626..3040)
                     /locus_tag="PLP58_11225"
                     /inference="COORDINATES: protein motif:HMM:NF019219.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1549 domain-containing protein"
                     /protein_id="HRJ09134.1"
                     /translation="MNRLSSLVALLLASSVHAAVDFNHQIVPLIRKHCGECHTGDKLK
                     GGFSMNDRAALLHGSENGPVVEPGKTEQSLLLEIVSTTDEDLRMPPKGDGLSADEVAL
                     LKQWIAEGLPWEPGFAFQAPAYEPPLKPRAVALPAAVDGRDHPVDRLMDAYLKKQNLP
                     RAEPASDSTFLRRAHLDLIGLLPSQEEVEAFLKEASPDKRARLVKSLLARDVDYTEHW
                     LTFWNDLLRNDYGGTGFITGGRKQISKWLYEALVTNKPFDQFARELIAPPSDESRGFI
                     DGIKWRGEVSAGQTVEIQFAQSLGQSFLGINLKCASCHDSFIDRWKLDEAYGLAAIYA
                     EQPLEVHRCDKPVGRTAQAAWLFPELGNVDPQAPRAKRLGQLAALMTHPENGRFTRTL
                     VNRLWHRLMGHGIVHPLDAMQSEPWSTDLLDYLAHHFQQNGYDLKKTLEHIATSQTYQ
                     ARSEILSDADSAFAFKGPRAKRLSAEQFVDAVWQLTGTAPKKMDAPVFRAKPDPAAAQ
                     AIALTGQWIWGSSAAEGKTPPAGETILLRANWKLDADPVSGAAILTCDNEFTLYLNGR
                     KVTSGDNWNQVTAVALHDKLKQGNNPIVVIAKNAGKGPNAAGLYFQAQVKLADGQEAT
                     LSSDATWQFSPSANAGKEGRLGALPNNFKPVTLVKALPVWTKSLTQQGPALLAQGSTS
                     GDRMIRAALVKSDFLMRSLGRPNRDQIVSMRPGDLTTLEALDLANGQPLTTALDQGAA
                     KLATQTWPSPTALITHLYTQTLARHPTPEELATARDLLGTKPTPAPIADLLWALLMQP
                     EFLYVQ"
     gene            complement(3143..4582)
                     /locus_tag="PLP58_11230"
     CDS             complement(3143..4582)
                     /locus_tag="PLP58_11230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009965585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1501 domain-containing protein"
                     /protein_id="HRJ09135.1"
                     /translation="MNSPLLHSLSRRAFLTQTSTGVGLAALGSMLDSRAAHAAPTLAG
                     FPHRPIKAKRVIYLFQSGAPSQMDLFDPKPKMAAYFQKDLPESIRKGQRLTTMTSGQK
                     SFPIAPSIFKFAEHGESRATISELMPHIASVADELCFIKSMHTEAINHDPAITFFQTG
                     RQLAGFPSMGSWLSYGLGSENQDLPAYVVLTSFGSGRKDDQPLYDRLWGSGFLPTQHS
                     GVKFRNTGDPVLYLSNPAGMDRALRRDTLDQLSALNQHRFQQIADPEIEARIAQYEMA
                     FRMQASVPDLTDTSKEPESVLDLYGPDVKRPGSYAANCLLARRLAERDVRFIQIFHMG
                     WDHHGGLPNAIKGQCQDTDQATAGLIKDLKQRGLLDDTLIVWGGEFGRTIYSQGTLTE
                     TNYGRDHHPRCFSLFLAGGGIKPGFTLGETDDFCYNIVKDPVSIFDLHATMLHLMGID
                     HNRLTHKFQGLDARLTGVEGAKVVKQILG"
     gene            complement(4588..7761)
                     /locus_tag="PLP58_11235"
     CDS             complement(4588..7761)
                     /locus_tag="PLP58_11235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009965584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PSD1 and planctomycete cytochrome C
                     domain-containing protein"
                     /protein_id="HRJ09136.1"
                     /translation="MPATNVMCLQKSIHWFPLSLILLAASCDLPAAERVDFNRDIRPI
                     LTENCTYCHGPDANHRKADLRLDVREDAIQAGALVPGDAAKSEIIARILTTDEDDLMP
                     PPDSHKKLTPAEKELLQRWVAEGAEYQGHWAFEKPVRPAVPEIPKAKTSVRNAIDAFV
                     LARLQQEKLGFSPEADARSLARRLSLDLTGLPPAPALVEAFAADPSDAAYEALVDRLL
                     QSPAYGEHMAAQWLDFARYADSHGFQTDSSRSMWPWRDWVIRAFNDNLPFDQFTIEQL
                     AGDLLPDARLDQIVATGFHRNHRINGEGGIIDEEWRLENIIDRVDTTGATWMGLTLGC
                     ARCHDHKYDPITQREFYEMFAFFNNIEETGVIRGASNRTGGNPGPSVKVPDAQQQKEL
                     ATLKAAVTAAEAAVLAAEKTLPALLASWEPEFQRELAQSKPLWQPLEPAKVSSTHGTT
                     FTRQEDGTWLTGGPTPTHDTFIIESPLAAGLLSGIRLDVLPDPSLPAGSVGRNDNGNF
                     VLTQVEAEITAPSLAQPVKLVYTEAEADYSQKGWGIESLIPAEPAKKAKRAATKGSNR
                     GRGWAVDGNTRREPNRAMLLTSEAVPVPADATLTVRLVHRTLAKHQIGRFKLNITSAP
                     PETLTLDGDGAAPQDIRAILAIAPDKRDTKQKARLTAFFRAGFDSPVKRADAALAAAR
                     QKHTAFETALPDVMVMKELPEPRPARILIRGEYDKPGDAVTAATPAVLPPLPEGAKRD
                     RLALARWLVSPEHPLTARVWVNRQWERFFGLGLVKSSENLGVQSDPPSHPELLDWLAT
                     EFMATGWDMKRLQKLIVMSATYRQTASRAHLPAELLAKDPENRLLARGPRQRLSAEVI
                     RDQALAISGLLDPKIGGPSARPYMPEGVWDETSRYGDLRGYKPDTERGLYRRTLYTIW
                     KRTAAPPTMLLFDSPSRETCTVKRARTNTPLQALALLNEVTYLEAARVFAQRMIHEGG
                     ATPAERITWAFQQALSRPPDARESAILTEGLDRHLARYQAAPAEAEALLAQGHAPLPA
                     DLEPALLAAYTVTANVVLNLDELLNKE"
     gene            complement(7845..8735)
                     /locus_tag="PLP58_11240"
     CDS             complement(7845..8735)
                     /locus_tag="PLP58_11240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ09137.1"
                     /translation="MKPAFCLLIPACILVLCPAAVAQDAALASQLARAYGAWRAAMQS
                     GDAKAWAAAITMHRQVTTRNLIVSQRKNWPAAVFEVPLDPPDISSLRLLEADAVGETA
                     HLLFFGRVDMGGGPGVIPESVLMLKFFNERGTWKFDSSKLLRLDDQPEIAQQIKAGGR
                     LDFLDYPEFTPPGKSPPVPKICGIPDHVTGCTFQSLGYQTRMIINGHDYPVMTDHAEK
                     IFVIGGLKNGANEIILEVKPVEIPPEGIRLLQLDFFVLAGKEGRQPVRVFHYEKNDPN
                     LTGIQRLTLTITPEILSKGL"
     gene            8832..9701
                     /locus_tag="PLP58_11245"
     CDS             8832..9701
                     /locus_tag="PLP58_11245"
                     /inference="COORDINATES: protein motif:HMM:NF019474.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HRJ09138.1"
                     /translation="METPGAGPHKLLPLDRNRELLKTAREETYKVVGDVSLPVFIWEP
                     GPDKAPPYPKSVAAFFFSSGWDNGQVAQFAPHCVYFASRGMLTLAFDYRVANRNHGGP
                     LEAIADARSAIRWIRLNAVELGINPGKIIGVGGSGGAHAITSAVMLEGFDDANDAVDC
                     SPAPNALVLFNPVLDTSKHGFGLDRFPDAGVAKSANLIRAIRPGLPPMLLMHGTADRV
                     VPFAGTYEFAKKNSKKKNFCRVIEFEGQGHGFFNFNLSFEMYEATLMAMDEFFVELGF
                     LEPDPDAGLGKVD"
     gene            complement(9705..11435)
                     /locus_tag="PLP58_11250"
     CDS             complement(9705..11435)
                     /locus_tag="PLP58_11250"
                     /inference="COORDINATES: protein motif:HMM:NF028132.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ09139.1"
                     /translation="MPDLPILAAIIPTVIVALACGLRGGLTKKSRRRDAAGLLGLGFA
                     KLLLLALPLAPLIDACINAHPLARGGWPVWMALLAVTSQCVLLVSGTADILRALGLWL
                     GGMPPEITRAPFASDTFSGLWQRFCHPLRKRFGIFAGMALFLATMLLANGMRAGAMSW
                     VALHLTLPALERLRGGRSLVPSFVPLPLRAVFVILVFFLSSLLLISGGMVDAWNQWQL
                     MFGLGVTNSFTLLLDARLSTDWPLCILWLSVFSALTLTGLRRFWSRHRWTALAGGGAL
                     GLAALIAGPPLNDWPAWSAQQALVSRVKYEIFNEGGSRVVAGAGGWLYDAAELDRSTR
                     SDTPEGFAAAMLALQEQLAKKSATLLVVPVPGKLALHPEPVLPAKYAAPLQPHGLRAI
                     LERLRAAGAQVIDPAQTLWDTRRRRDSYFRRDSHWTPETMKETALIVAKHIRRHWPRL
                     ANDETPLINATIIEREHAGDLALRLAHGNAEWFEPEHATLLAIKGLDSSRDSPVLLAG
                     GDLLRVYDDPALSFGNSDGIPQSAGFAQQLSALLARPLDVADEAELLADTTRVSEKQL
                     VIWLLHAWRL"
     gene            complement(11505..14000)
                     /locus_tag="PLP58_11255"
     CDS             complement(11505..14000)
                     /locus_tag="PLP58_11255"
                     /inference="COORDINATES: protein motif:HMM:NF012492.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HRJ09140.1"
                     /translation="MLESLNTLKLPDAWQHQAVNHLRAGSDVVVSAPTGAGKTYIFEL
                     LHQSRSLTGQAVYTVPTRALANDKYAEWRDAGWNVGIATGDVSENIGAPVIVATLETQ
                     LERLVRGEGPALLVVDEYQMIADGARGSHYEAAVALAPVETRLLLLSGSVANPEDVVA
                     WLRRLGRKAEVVITRERPVPLEEVPWEALPQRMARHFEHFWPKFAASVMLADLAPLLI
                     FSPRRREAESIARRLAADLPVGDPLELTPQQRAVCGKELCNLLERRIAFHHSGLSYAA
                     RAGVIEPLAKAGQLRVIVATMGLAAGINFSVRSVHVAATTFHDGKAEHKLSPDELLQM
                     YGRAGRRGLDDKGHVVTTKQSPTLMDARPARLNRGSLLAWPIFLRVMKHAALTGGNPF
                     EAAEHFAGRLYAKVPPLLGLEENAGEGALAGGLQEAKALFGLEATEKQIFNSQGEWER
                     RANVPDEMLPLKDAWLASMERIAPALSVPGFVAKFAHGIGRVGRISKEHYGVEIPLGM
                     KHEDGASLSPTKNLRRLLKLPRKLENLPIEEIEVLHSDTIAAAILAAHPELPQRPLPR
                     LHGIVEKDRLHFAVFELSDLLVPVYRDSHDKPLHQPKERTIMVRRDTSIHPEENPASP
                     DRQPRPGSPIHAWRALGLIDAEGVPTRRGEIFSFFQGGEGLAIVAALEDEGYRVTDLI
                     THMANLRSGSKLDLPTACDSERLAATCRNTYGYINHHGYLEHGLPVDYGEGAAELLTA
                     LLHPETPDAGELRRGVAEGDISRAYIEWLSLLRHIVHAPAHPWQRWLALQEEAGRILK
                     KHGKTLRHLFHLDLPPLTNKQRQSRPRHYLKTE"
     gene            complement(14052..14459)
                     /locus_tag="PLP58_11260"
     CDS             complement(14052..14459)
                     /locus_tag="PLP58_11260"
                     /inference="COORDINATES: protein motif:HMM:NF013970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain-containing protein"
                     /protein_id="HRJ09141.1"
                     /translation="MSFRILPDSSAWIAYFRNSDVPTADLVEKLIEVEADICLCGVVI
                     TEVLQGVRSDKDHRLIQDIFNTPEYLEMDRLTFEFAAEIYRACRAKGFTIRSTVDCII
                     AATAIQHDAHLLHCDRDYEAIARFYPLRIYGGQ"
     gene            complement(14449..14757)
                     /locus_tag="PLP58_11265"
     CDS             complement(14449..14757)
                     /locus_tag="PLP58_11265"
                     /inference="COORDINATES: protein motif:HMM:NF021474.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapB family
                     antitoxin"
                     /protein_id="HRJ09142.1"
                     /translation="MSKRASKYKAKRSPDADHWIAMNEAEAKAEKEDKETMRTNIIME
                     TPLVYAAQEITGIKTKAGVVHHALKELVRRERMKQLLALRGKVAWDGDLDEMRRTREF
                     "
     gene            14865..16082
                     /locus_tag="PLP58_11270"
     CDS             14865..16082
                     /locus_tag="PLP58_11270"
                     /inference="COORDINATES: protein motif:HMM:NF024907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VCBS repeat-containing protein"
                     /protein_id="HRJ09143.1"
                     /translation="MKLPLLLAALAVPAFAATPTFKKITLTDEFVSEGAHFADFDKDG
                     HNDICAGPYIWKGPEFKERLGYTAPSEKPYDPGKGYSDYFLTYTYDFNNDGWQDILVY
                     SWPGKETWVFENPQNKPGHWTRHTIFDVTDNESPALGDMNGDGKPELICHTSSGSQPP
                     SGGRLGFAEIDWSNPLGKARFRPITPVTPENDKKYFRYTHGYGFGDVNGDGRPDLLTK
                     DGWFEQPADMKEDKDWAFHPGPFVPEGERGGSFLLVYDVNGDGRNDVITSHNAHGYGF
                     GWYEQNADGSFTPHKLMGATIEESPIGVKFSQLHAMQLADVDGDGVLDVVTGKRRWAH
                     GPLKDDEPNAPPVLYWFQIKRDGKGGAQFIPHLIDDASGVGTQVTPGDVNKDGKMDVV
                     VGNKMGVFVLLQE"
     gene            16149..>17471
                     /locus_tag="PLP58_11275"
     CDS             16149..>17471
                     /locus_tag="PLP58_11275"
                     /inference="COORDINATES: protein motif:HMM:NF012318.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HRJ09144.1"
                     /translation="MRLNALLFLTFSAVALAAKSVEEIAAEVKPSVVKISQVGREGFD
                     GLGTGFVVSADGLIATNLHVIGEARQLEVETADGRKHEVVEVTATDSHWDLALLRVAS
                     KDLQPLPLGDNSTIQQGQPIVAMGNPQGLAFSVVDGVVSAYPDLIDDIPMIRLAVPIE
                     KGNSGGPLLDREGRVLGILTLKSARTENLGFAMPVNELKRMIESPNPVPMRRWLTIGV
                     LNPKLWQPLFGSRWTQRAGIIQAATPGSGFGGRSLCLWQADTPPEVFETSVNVKLDSE
                     SGAAGLVFCADGGDRHYGFYPSGGKLRLTRFEGADVYAWTILADVPAEAYRPGEWNHL
                     RVRVDQEKITCWVNGQVILTQEDTGLRGGRAGLCKFRNTVAEFRQFRVGTDLADKPLP
                     PAVAGKVSAALEAFAQSPAAREDTLATLLDQPAASRRLLLERRRELERQ"
BASE COUNT         2985 a         5121 c         6035 g         3330 t
ORIGIN      
        1 agggcttgct tgtggttcgg cagcagtccc agggctttgc ggaagcagta ggccgccttt
       61 tcttcgtgtt cggtgccgcc gagttcctca tgcaagttcg cgcgctgcat ccagaggtat
      121 tcgagtttcg gctcgcgctg ggtggcctgc ttggcggcct ccagggcggc ttctttgtcc
      181 cccagggatt tgagcgcaaa ggccttgttg aaggcgcagt agggggtgcc gggccgcagc
      241 gtctccagct cgtccaggag gcgaagcgtc gctgtcatgt caccggattc gagggcctct
      301 cccaactcgt ctccccgctc gatgaaggtc ttcacggcgt cccactcctc gctccatccg
      361 gtgcccgcct gacgcaggcg ctcaatgccc tcaagcaacc gctcgaaggg gaaatgcttt
      421 tgggggtcga tctttttgtc gtcctccttg ggccagggca ggggcggctt gatgccccag
      481 aagagcaggt cgtgatgctc tgttttcagc cagcgaaaat tcgattgagc ggagaagtcg
      541 ggcttcatgg tcaatggcgg attctggccg ttttacccga aagacatcgc ctcaggcaag
      601 cagaatccgt caagacccag gatggctact ggacgtagag aaactcgggc tgcatgagga
      661 gggcccagag gaggtcggcg atgggggcgg gggtgggctt ggtgccgagg aggtctcggg
      721 cagtggcgag ttcttcgggg gtggggtggc gggcgagggt ctgggtgtag aggtgggtga
      781 tgagggcggt ggggctgggc caggtttggg tggcgagttt ggcggcgcct tgatcgaggg
      841 cggtggtgag gggctggccg ttggcgaggt cgagcgcttc gagggtggtc aagtcgccgg
      901 ggcgcatgga gacgatttga tcgcggttgg gacggccgag ggagcgcatg aggaaatcgc
      961 tcttcacaag ggcggcgcgg atcatgcggt cgccgctggt gctgccttgg gcgagcaggg
     1021 cgggaccttg ctgagtgagg gacttggtcc agacgggcag ggctttgacg agggtgacgg
     1081 gcttgaagtt gtttggcagc gcgccgagtc ggccttcttt gccggcgttg gcgctggggc
     1141 tgaactgcca ggtcgcgtcg ctgctgaggg tggcttcctg accgtcggcg agtttgacct
     1201 gggcttggaa atacaggccg gcggcgttgg ggcctttacc ggcgtttttg gcgatgacga
     1261 cgatggggtt gttgccctgt ttgagcttgt cgtgcagggc gacggcggtg acttggttcc
     1321 agttgtcgcc ggaggtgact ttgcggccgt ttaggtagag ggtgaattcg ttgtcgcagg
     1381 taagaatggc ggcgccgctg acggggtcgg cgtcgagctt ccagttggcg cgcaggagaa
     1441 tcgtttcacc ggcgggcgga gttttgcctt cggcggcgga gctgccccag atccactggc
     1501 cagtgagagc gatggcctgg gcagcagcgg ggtcgggttt ggcgcggaag acgggggcgt
     1561 ccattttttt cggggcggtg ccggtgagct gccagacggc gtccacgaac tgctcggcgc
     1621 tgagacgctt ggctcgcggg cctttaaagg cgaaggcgct gtcggcatcg ctgaggatct
     1681 cgctgcgcgc ctgataggtc tgcgaggtgg cgatgtgctc cagggtcttt ttgaggtcgt
     1741 aaccgttctg ctggaagtga tgggcgaggt aatcgagaag gtcggtgctc cagggctcgc
     1801 tctgcatggc gtcgagcgga tgcacgatgc catggcccat gaggcggtgc cagaggcggt
     1861 tgacgagggt gcgggtgaag cggccgtttt ccgggtgggt catcagcgcg gcgagctggc
     1921 cgaggcgctt ggcgcgcggg gcctgcgggt ccacgttgcc gagttcgggg aaaagccagg
     1981 cggcctgggc ggtgcggccc acgggtttgt cgcagcgatg aacctcgagg ggctgctcgg
     2041 cgtagatggc ggcgaggcca taggcttcat cgagcttcca gcggtcaatg aagctgtcgt
     2101 ggcaggaggc gcacttcagg ttgatgccga gaaaggactg gcccaggctt tgcgcgaact
     2161 ggatttccac agtctgcccc gcgctgacct cgccgcgcca tttgatgccg tcaatgaagc
     2221 cgcggctttc atcgctgggc ggcgcgatga gttcgcgcgc gaactggtcg aagggtttgt
     2281 tggtgacgag ggcttcgtag agccatttgc tgatctgctt gcgccctccg gtgatgaagc
     2341 cagtgccgcc gtagtcgttg cggaggaggt cgttccaaaa ggtgagccag tgctcggtgt
     2401 agtccacatc gcgggcgagc agtgacttca cgaggcgcgc gcgtttgtcg ggcgaggctt
     2461 ctttgaggaa tgcctcgacc tcctcctgag agggcagcag gccgatgaga tcgaggtggg
     2521 cgcggcggag gaaggtgctg tcgctggcgg gctcggcgcg cgggaggttt tgcttcttca
     2581 aataggcgtc catcaggcgg tccacgggat ggtcgcggcc atccacagca gctggcaggg
     2641 cgacagcgcg gggtttgagc gggggctcgt aggcgggggc ttgaaaggcg aagccgggct
     2701 cccagggcag gccctcggcg atccattgct tgagcagggc gacttcgtcg gcggagaggc
     2761 catcgccctt gggcggcatg cgcaggtcct cgtcggtggt ggagacgatt tcgagcagca
     2821 ggctttgctc cgtcttgccc ggttcaacga cggggccatt ctcgctgccg tggaggagag
     2881 cggcgcggtc gttcatggag aagccgcctt tcagcttgtc gccggtatgg cactcgccgc
     2941 agtgtttgcg gatgaggggg acgatctgat gattgaagtc caccgccgcg tggacggagg
     3001 acgcgagcag aagagcgacg aggctggaga gtcggttcac ggcctttaca acgttgcatg
     3061 ggatcagaac catccgcaaa gcatgtgtct gatccggcag tggactggat cttgtgaact
     3121 acgaccgaaa tcgcagctgc attcacccca ggatttgctt caccaccttg gcgccttcca
     3181 cgccggtgag gcgggcgtcg aggccttgga acttgtgggt gaggcggttg tggtctatgc
     3241 ccatgaggtg gagcatggtg gcgtggagat cgaagatgct gacggggtct ttgacgatgt
     3301 tgtagcagaa gtcgtcggtc tctccgagcg tgaagccggg cttgatgccg ccgccggcta
     3361 ggaagaggga gaagcagcgc gggtggtggt cgcgtccgta gttggtctcg gtgagcgtgc
     3421 cctgggagta gatggtgcgg ccaaactcgc cgccccagac gatgagggta tcatcgagca
     3481 ggccgcgctg cttgaggtcc ttgatgaggc cggcggtggc ctggtcggtg tcctggcact
     3541 ggcctttgat ggcattgggc aggccgccgt ggtgatccca tcccatgtgg aagatctgga
     3601 tgaagcgcac gtcgcgctcg gcgagacggc gggcgagcag gcagttggcg gcgtagctgc
     3661 cggggcgttt cacatcgggg ccgtaaaggt cgagcacgct ctccggctcc ttcgaggtgt
     3721 cggtcaggtc gggcacgctg gcctgcatgc ggaaagccat ctcgtactgg gcgatgcggg
     3781 cctcaatctc gggatcggcg atctgctgga agcggtgctg gttgagcgcg ctgagctggt
     3841 cgagcgtgtc gcgccgcagg gcgcggtcca taccggcggg attggagagg taaaggacgg
     3901 gatcgccggt gttgcggaat ttgacgccgc tgtgctgggt gggcaggaag ccgctgcccc
     3961 agaggcggtc gtagagcggc tggtcgtcct tgcgcccgct gccgaaggag gtcaaaacga
     4021 cgtaagcggg caggtcctgg ttttcgctgc cgaggccgta actgagccag gagcccatgc
     4081 tggggaaacc ggcgagctgg cgcccggtct ggaagaaggt gatggccggg tcatggttga
     4141 tggcctcagt gtgcatggac ttgatgaagc acaactcgtc cgccacggag gcgatgtgcg
     4201 gcatcagctc gctgatcgtg gcgcggctct cgccatgctc ggcgaacttg aagatgctcg
     4261 gcgcgatggg aaaactcttc tgccccgagg tcatcgtggt gagccgctgg cctttgcgga
     4321 tggactcggg caggtccttc tgaaagtagg ccgccatctt cggcttcgga tcgaagaggt
     4381 ccatctgcga cggcgcgccg gactgaaaga ggtagataac gcgcttggct ttgatcggtc
     4441 ggtgtggaaa gccggccagc gtgggcgcgg cgtgggcagc gcggctgtcg agcattgaac
     4501 ccaaagccgc caatccaacc ccggtggagg tttgagtgag aaatgcgcga cgcgagagcg
     4561 agtggagcag tggagagttc atcgtgctta ttctttgttg agcagttcat ccaaattcag
     4621 caccacattc gcggtgacgg tgtaggcggc gagcagggcg ggctcaaggt cggcggggag
     4681 cggtgcgtgg ccttgtgcga ggagggcttc ggcttcggcg ggggcggctt ggtagcgggc
     4741 gaggtggcgg tcgaggcctt cggtgaggat ggcggactcg cgagcgtcgg gcggacggga
     4801 cagggcttgc tggaaggccc aggtgattcg ctcggcaggg gtggcgccgc cttcatggat
     4861 catgcgctgg gcgaagacgc gtgcggcttc aaggtaggtg acttcgttga gcagggccag
     4921 ggcctgcaac ggggtgttgg tgcgggcgcg tttgacggtg caggtctcgc gcgaggggga
     4981 gtcaaagagc agcattgtcg gcggtgcggc ggtgcgtttc cagatggtgt aaagcgtgcg
     5041 gcggtagagg ccgcgctcgg tgtcgggctt ataaccgcgc aggtcgccgt agcggctggt
     5101 ctcgtcccag acgccctcgg gcatgtaagg ccgcgcgctg ggaccgccga ttttcggatc
     5161 gagcaggccg ctgatggcga gggcctgatc gcgaatgact tcggcgctga ggcgctggcg
     5221 cgggccgcgg gcgagcaggc ggttctcggg gtctttggcg agcagttcgg cgggcaggtg
     5281 cgcgcggctg gcggtctggc gataagtggc gctcatgacg atgagctttt gcagccgctt
     5341 catgtcccag ccggtggcca tgaattcggt ggcgagccag tcgagcagtt ccgggtggct
     5401 gggcgggtcg gactgaacgc cgaggttctc gctggacttg acgagcccga ggccaaagaa
     5461 gcgctcccat tgccggttca cccagacgcg ggcggtgagc gggtgttcgg gcgagaccag
     5521 ccagcgcgcg agagcgagcc ggtcgcgttt tgcaccctcg ggcagcggcg gcaacacggc
     5581 aggcgtggcg gcggtgacgg cgtcgccggg tttgtcgtac tcgccgcgga tgaggatgcg
     5641 cgcggggcgc ggttcgggca gctccttcat gaccatgacg tcgggcaggg cggtctcaaa
     5701 agcggtgtgc ttttgacgtg cggcagcgag cgcggcatcg gcgcgtttca ccgggctgtc
     5761 aaacccggcg cggaagaagg cggtcagtcg cgctttctgt ttggtgtcgc gcttgtcagg
     5821 cgcgatggcg aggatggcgc gaatgtcctg cggggcggcg ccgtcgccat ccagagtgag
     5881 ggtttccggc ggggcgctgg tgatgttgag cttgaagcgg ccgatctggt gcttggcaag
     5941 tgtgcggtga acgaggcgca cggtgagagt ggcatcggca ggcacgggaa cggcttcgga
     6001 ggtgagcagc atggcgcggt tcggttcacg gcgggtgttg ccatccacgg cccagcctct
     6061 gccgcgattg ctgcctttgg tggcggcgcg cttggctttc ttggcgggct cggcggggat
     6121 gagggactcg atgccccagc ctttttgaga gtagtcggcc tcggcttcgg tgtagacgag
     6181 tttcacgggc tgggcgaggc tgggtgcggt gatttcggct tcgacttggg tgaggacaaa
     6241 gttgccgttg tcattgcgac cgacactgcc ggcggggaga gagggatcgg gcagcacgtc
     6301 gagccggatg ccgctgagca gacctgcggc aagcggggac tcgatgatga aggtgtcgtg
     6361 cgtgggagtg gggccgccgg tgagccaggt gccgtcttcc tggcgtgtga aggtggtgcc
     6421 gtgggtggag ctgactttgg cgggttccag cggctgccaa agcggtttgc tctgcgccag
     6481 ttcgcgctgg aactccggct cccaagaggc gagcagggcc ggcagcgttt tctccgccgc
     6541 cagcacagcg gcctcggcag cggtgacggc ggcctttaaa gtcgcgagtt ctttctgctg
     6601 ctgcgcgtcc ggcactttga cgctggggcc ggggttgccg ccggtgcggt tcgaggcgcc
     6661 gcggatgacg ccggtttcct cgatgttgtt gaagaaggcg aacatctcgt agaactcgcg
     6721 ctgggtgatg ggatcgtact tatggtcgtg gcagcgcgcg cagccgaggg tgaggcccat
     6781 ccaggtggcg ccggtggtgt ccacgcggtc aatgatgttt tcgagccgcc actcctcgtc
     6841 aatgatgccg ccctcgccat tgatgcggtg gttgcgatgg aacccggtgg cgacgatttg
     6901 atcgaggcgc gcgtcgggca ggaggtcacc cgcgagctgc tcgatggtga actggtcaaa
     6961 gggcaggttg tcattgaacg cgcggatcac ccagtcccgc cacggccaca tgctgcggga
     7021 ggagtcggtc tggaagccgt ggctgtccgc gtaacgggcg aagtcgagcc actgcgcggc
     7081 catgtgctcg ccataggccg gggattgcaa aagccggtcc accagcgcct cgtaagcagc
     7141 gtcggaagga tcggcggcga aggcttcgac aagcgcgggc gcaggcggca ggccagtgag
     7201 gtcgaggctg aggcggcgag cgagcgaacg agcgtctgct tccggagaga aaccgagctt
     7261 ttcctgctga agacgggcga gcacaaaggc atcaatggca ttgcgcacgc tggtcttggc
     7321 cttggggatc tcgggcaccg ccgggcggac gggcttttca aaggcccagt ggccctggta
     7381 ttccgctcct tccgccaccc agcgctggag cagttccttt tcggctggcg tgagcttttt
     7441 gtgcgagtcc ggcggcggca tgaggtcgtc ctcatccgtg gtcaggatgc gggcgatgat
     7501 ctcgctcttg gcggcgtcgc ccggcacaag agcgcccgcc tggatcgcgt cctcgcgcac
     7561 atcgagtcgg aggtcggcct tgcgatgatt ggcgtccggg ccgtggcagt aggtgcagtt
     7621 ttccgtgagg atgggcctga tgtcgcggtt aaagtccacg cgctcggcgg cgggcaggtc
     7681 gcatgatgca gccagtaaaa tcaagctgag agggaaccaa tgtatagatt tttgcaggca
     7741 catgacgttg gtggcaggca tgtgcatcgc ctgccgggac gggatgagac gcggcaaaga
     7801 aggccatctt tccattctgc gccatacttg ccggcgcggc aaactcacaa gcccttgctc
     7861 aaaatttccg gtgtgatggt caaggtgagc cgctgtatgc cggtgaggtt ggggtcattc
     7921 ttttcataat gaaagacgcg caccggctgg cggccttcct ttccggcaag gacaaagaag
     7981 tcgagttgca agaggcggat gccttccggc gggatttcga ccggcttgac ttcgaggatg
     8041 atttcgttgg cgccattttt gaggccgcca atgacaaaga tcttttccgc gtggtcggtc
     8101 atcacgggat aatcgtggcc gttgatgatc atgcgcgtct ggtagccgag agattgaaag
     8161 gtgcagccgg tcacatgatc ggggatgccg cagattttcg gcacgggcgg ggatttgcct
     8221 ggcggtgtga actcggggta atcgaggaag tcaagacggc ctcccgcttt gatctgctgg
     8281 gcaatctccg gctggtcgtc gaggcggagg agcttgctgc tgtcgaattt ccaagttccc
     8341 cgctcgttga aaaatttcag catgaggacg ctttcaggaa tgacgcccgg tccgccgccc
     8401 atgtccacgc ggccaaaaaa aagcaggtgc gcggtctcgc cgacggcatc cgcctccagc
     8461 aggcgcaggc tggaaatgtc cggcgggtcg aggggcactt cgaagacagc ggcgggccag
     8521 tttttgcgct ggctgacgat gaggttccgt gtggtcacct gccggtgcat ggtgatggca
     8581 gccgcccatg ctttggcatc gcctgactgc atggctgcgc gccatgcgcc gtaagcgcgc
     8641 gcgagttggg aggccagcgc ggcatcctgc gcgactgcgg cggggcaaag caccaggatg
     8701 caggccggga tcagaaggca gaaagcgggt ttcatggcat tgcaatacca cgcctcgtga
     8761 catgcattcc aggaaaagat gttgctgccg gtgtgttttt cgcaaaactc gcgctttcga
     8821 cacactttga catggaaact cccggcgcag gtccacacaa gctcctcccc ctcgaccgca
     8881 atcgggaatt gctgaaaacg gcgcgcgagg aaacctacaa ggtggtgggc gatgtgagcc
     8941 tgccggtttt catctgggag cccgggccgg acaaggcgcc tccgtatcca aaatcggtgg
     9001 cggcgttctt tttcagcagc gggtgggaca acgggcaagt cgcgcagttc gcgccgcact
     9061 gcgtttactt cgcctcgcgc ggaatgctga cgctggcgtt tgactaccgt gtggcgaatc
     9121 gcaatcacgg cggaccgctg gaggcgatcg ctgatgcgcg ctcggccatc cgctggattc
     9181 gcctcaatgc cgtggaactg ggcatcaatc ccggcaagat catcggagtc ggcggcagtg
     9241 gcggcgctca tgccatcact tcggcggtca tgctggaggg cttcgatgac gcgaatgacg
     9301 cggtggattg cagccccgcg ccgaacgcgc tcgtgctttt caatcctgtg ctcgacacgt
     9361 cgaagcacgg ctttgggctg gaccgtttcc ccgacgccgg agtggccaaa agcgcgaatc
     9421 tcatccgcgc gatccgcccc ggcctgccgc cgatgctatt gatgcacggc accgccgacc
     9481 gcgtggttcc ttttgcgggc acctacgagt ttgcgaagaa gaattcgaag aagaaaaatt
     9541 tctgccgggt cattgagttc gaggggcagg ggcacggctt tttcaacttc aatctctcct
     9601 tcgagatgta cgaggccacg ctgatggcga tggacgagtt ctttgtcgaa ctgggcttcc
     9661 tggagcctga ccccgacgcc ggtctgggca aggtggactg agcttcaaag cctccacgca
     9721 tgcagcagcc agatgacgag ctgcttttca gagacgcggg tggtgtccgc gagcagttcg
     9781 gcttcatcgg ctacatcaag cggacgcgcg aggagggcgg agagctgttg tgcgaaaccg
     9841 gcgctttgcg ggatgccatc ggagttgccg aagctcagcg cgggatcgtc gtagactcgg
     9901 agcaggtcgc cgcccgcgag cagcacgggc gagtcccggc tggaatcaag ccccttgatg
     9961 gccagcagcg tggcatgttc cggctcgaac cattccgcat ttccgtgcgc aagtcgcagc
    10021 gcaaggtcgc cggcgtgctc gcgctctatg atggtggcgt tgatgagcgg tgtctcgtca
    10081 ttggccaggc ggggccagtg ccggcggatg tgtttcgcca cgatcagggc tgtctccttc
    10141 atggtttccg gcgtccagtg gctgtcccgg cggaaataac tgtcgcgcct gcggcgcgtg
    10201 tcccagagtg tctgcgcggg gtcaatcacc tgcgcgccgg cggcgcgaag gcgctcgagg
    10261 atggcgcgca ggccgtgcgg ttgcagaggc gcggcgtatt tcgcgggcag caccggctca
    10321 ggatgcagag ccagttttcc cggcacaggc acgacgagca gggtggcgct tttttttgcc
    10381 agttgctcct ggagcgcgag catggcggct gcaaagcctt cgggtgtgtc agagcgggtg
    10441 ctgcggtcca actctgcggc atcgtaaagc cagccgcccg cacctgcgac gacgcggctt
    10501 ccgccctcat tgaatatctc atatttgacg cgcgacacga gcgcctgctg cgcgctccag
    10561 gcgggccagt cattcagagg cggtccggcg atcagcgccg cgagtccgag cgcaccgccg
    10621 ccagccagag ccgtccaacg atgacgcgac caaaacctgc gcaggccggt gagcgtgagg
    10681 gcactaaaca cggagagcca caaaatgcac agcggccaat cggtgctgag acgtgcgtca
    10741 agcaggagcg tgaagctgtt tgttacgcct aggccaaaca tgagctgcca ctgattccat
    10801 gcatcaacca ttccgccgct gatcaggagc aggctcgata ggaaaaaaac gaggatgaca
    10861 aacactgcgc ggagaggcag gggcacgaaa gagggcacca ggctgcgccc gccgcgcaga
    10921 cgctccagtg ctggcagagt caggtgaaga gctacccatg acatggcacc agcgcgcatg
    10981 ccatttgcca gtagcatggt ggcaagaaag agcgccattc ccgcgaagat gccaaagcgt
    11041 tttctcaaag gatggcaaaa gcgctgccac agcccgctaa aggtgtcaga ggcgaagggg
    11101 gcacgagtga tttcaggcgg catgccgccg agccagagtc ccagcgcgcg cagaatgtcc
    11161 gctgtgccgg agacaagcaa aacacactgg ctggtgacag ccagcagggc catccagacg
    11221 ggccatccgc cgcgcgcaag cgggtgcgca ttgatgcaag catcaatcaa tggcgcaagc
    11281 gggagggcga gcagcagcag cttggcaaat ccaagaccga gaagccccgc cgcatctctt
    11341 cgcctgcttt ttttcgttag cccgccgcgc aaaccacagg ccagcgcgac gatgactgtg
    11401 ggaatgatgg cagctaggat tggcaggtct ggcacggtgc ttgcggtgga atttgaaacg
    11461 actaatttgg gaaatgagga tttcgagttt cgaaattcga tttttcactc ggtcttgagg
    11521 taatgccgcg gccggctttg gcgctgcttg ttggtgaggg gcgggaggtc gaggtgaaag
    11581 aggtgacgca gagtcttgcc gtgctttttc aatatgcgcc cggcctcttc ctggagggcc
    11641 agccagcgct gccaggggtg cgctggcgcg tggacgatgt gacgcagcag gctgagccac
    11701 tcgatgtagg cgcgtgaaat gtcaccctcg gcgactcccc ttcgcagctc gcccgcatcg
    11761 ggtgtctcgg gatggagcag ggcggtgagc agctcggcgg cgccctcgcc ataatccaca
    11821 ggcaggccgt gctccaggta gccgtggtgg ttgatgtagc cgtaggtgtt ccggcaggtg
    11881 gcggcaaggc gctcggagtc gcaggcggtg ggcagatcga gcttcgagcc gctgcgcaga
    11941 ttcgccatgt gcgtgatgag gtcggtgacg cggtagcctt cgtcttccaa agcggcgacg
    12001 atggccaagc cttcgccacc ttggaaaaag gaaaaaatct cgccgcgccg ggtgggcacg
    12061 ccctccgcgt caatgaggcc gagcgcgcgc caggcatgga ttggcgagcc ggggcgtggc
    12121 tggcggtcag gagaggctgg gttttcctct gggtgaatgg aggtgtcacg ccggaccatg
    12181 atggtgcgct ccttgggctg gtggagcggc ttgtcatggc tgtcgcgata gacggggacc
    12241 aggaggtcag agagctcgaa gacggcgaaa tgcaggcggt ctttttccac gatcccatgc
    12301 aggcggggca ggggtctctg tggcagttcg ggatgcgcgg cgaggatggc ggcggcgatg
    12361 gtgtcgctgt gcaggacttc gatttcttcg atgggcaggt tctcgagttt gcgcggtagt
    12421 tttaacaggc ggcgcaggtt tttggtgggg ctgagactcg ccccgtcttc atgcttcatg
    12481 ccgagcggta tctcgactcc gtaatgctct ttgctgatgc gccccacgcg cccgatgccg
    12541 tgcgcgaatt tggccacaaa accgggcaca ctgagcgcgg gcgcgatgcg ctccatgctc
    12601 gcgagccagg cgtctttgag cgggagcatc tcatcgggca cattcgcgcg ccgctcccac
    12661 tcgccctggg agttgaagat ttgcttctca gtggcctcga ggccgaagag cgccttggct
    12721 tcttgaaggc ctcccgccag cgcgccttcg ccggcgtttt cctccagtcc gagcaggggc
    12781 ggcaccttcg catacagccg gcctgcaaaa tgctcggcgg cttcgaaggg attgccgccg
    12841 gtcagcgcgg cgtgcttcat gacgcgcaga aagatgggcc atgccaaaag gctgccgcga
    12901 ttcaagcgcg cggggcgtgc atccatgagc gtggggcttt gcttcgtggt gacgacgtgg
    12961 cccttgtcat ccagcccgcg tctgcctgcg cggccataca tttgcagcag ctcatccggg
    13021 gagagcttgt gctccgcttt gccgtcatga aaggtcgtgg cggccacatg cacgctgcgc
    13081 acggagaaat tgatgcccgc cgcgagcccc atggtggcca cgatgacgcg gagctgcccg
    13141 gccttcgcca gcggctcaat gacacccgcc cgcgcggcat aggagaggcc gctgtgatga
    13201 aaggcaatgc ggcgctccag cagattgcac agctctttgc cacacacggc gcgctgctgc
    13261 ggcgtgagtt cgagcggatc accgacaggc aggtccgccg ccaaccgccg ggcgatgctt
    13321 tccgcctcgc gtcggcgggg tgaaaagatg agcagcggcg cgaggtccgc gagcatgacc
    13381 gaggcggcga attttggcca gaagtgctcg aagtgcctcg ccatgcgctg tggcagcgcc
    13441 tcccacggca cttcttctag cggcacgggg cgctctcggg tgatgaccac ttccgccttg
    13501 cgccccagcc gacgcagcca ggccaccacg tcttcaggat tcgccacgga gccactgagc
    13561 agcagcagcc gcgtctccac gggtgcgagc gccaccgccg cctcgtaatg actgccgcga
    13621 gcgccgtcgg cgatcatttg atactcgtcc accacgagca aagccggccc ctcgccgcgc
    13681 acgagccgct caagctgggt ctccagtgtg gcgacaatga caggcgcgcc gatgttctcg
    13741 gacacatccc cggtggcgat acccacattc cacccggcat cccgccactc ggcgtatttg
    13801 tcgttcgcca aagcgcgtgt cggcacggtg taaacggcct gccctgtcag cgagcgggac
    13861 tggtgcagca gctcgaagat gtaggtcttg cccgccccgg tcggcgcgct caccaccaca
    13921 tcactgcccg cgcgcaggtg gttgacggcc tggtgctgcc aggcgtcggg aagtttgagc
    13981 gtgttgaggg attcgagcat cttgccgcgt cagtgcatac gaacgcatga cgcggcgggc
    14041 aagcgtctcc ctcattggcc gccataaatc ctcagcggat aaaagcgcgc aatggcttcg
    14101 taatcacggt cacagtgcag caaatgcgcg tcgtgctgga tggccgtggc tgcgatgatg
    14161 cagtccaccg tgctgcggat ggtgaagcct ttggcccggc aggcacggta aatctccgcc
    14221 gcaaactcaa acgtcagccg gtccatttca agatactccg gcgtgttgaa gatgtcctga
    14281 atcaggcggt ggtctttgtc actgcgcacc ccttgcagca cctccgtgat cacaacgccg
    14341 cataaacaaa tatcggcctc gacttcgatg agtttttcca ccaggtcggc ggtgggcacg
    14401 tccgagttcc tgaagtaagc gatccaggcg ctggagtccg gcagaattct aaaactcacg
    14461 cgtcctcctc atctcatcga gatctccatc ccaggccacc ttgccccgca aggcgagcaa
    14521 ttgcttcatc cgctcccgcc ggacgagctc cttcaaggcg tggtgtacca cgccggcttt
    14581 ggtctttatg ccagtgattt cctgggcggc ataaacaagg ggcgtttcca tgatgatatt
    14641 ggttctcatg gtttccttat cttccttttc ggcttttgct tcggcttcgt tcatggcgat
    14701 ccagtggtcg gcgtcggggg acctcttggc tttgtatttg ctggctcttt tgctcataca
    14761 cactttttat ctcattcatg tgtatatcgc aactcgtttt ttcatttctc ccgcttcggg
    14821 catccatgca agattccagg tggccagctt gttccctcac gcccatgaag ttgcccctcc
    14881 tcctcgcagc cctcgctgtt ccggcttttg ccgccacgcc cactttcaag aaaatcaccc
    14941 tcaccgatga attcgtctcc gagggtgcgc acttcgcgga tttcgacaaa gacggtcaca
    15001 atgacatctg cgccgggcct tacatctgga agggaccgga gttcaaggag cgcctcggat
    15061 acaccgcccc ctccgagaag ccctatgatc cgggcaaagg ctacagcgat tatttcctga
    15121 cctacaccta cgacttcaac aacgacggct ggcaggacat cctcgtctat tcctggccgg
    15181 gcaaagaaac gtgggtcttc gaaaacccgc aaaacaaacc cggccactgg acccgccaca
    15241 ccatcttcga tgtcaccgac aacgaatccc cggcgctggg agacatgaac ggcgacggca
    15301 agcccgaact catctgccac accagctccg gcagccagcc cccctccggg gggcgcctcg
    15361 gttttgccga aattgactgg agcaatcctc tgggcaaagc ccgcttccgc cccatcactc
    15421 cagtcacccc cgagaatgac aaaaaatact tccgctacac ccacggctac ggctttggcg
    15481 atgtgaacgg cgatggtcgc cccgacctcc tcaccaagga cggctggttc gagcagcccg
    15541 ccgatatgaa ggaggacaaa gactgggctt ttcaccccgg ccccttcgtc ccggagggcg
    15601 agcgcggcgg ctccttcctg ctggtctatg acgtgaatgg cgacgggcgc aacgacgtga
    15661 tcaccagcca caacgcccac ggctatggct tcggctggta tgagcaaaac gccgacggca
    15721 gcttcacccc gcacaagctc atgggtgcca ccatcgagga aagccccatc ggtgtgaaat
    15781 tcagccagct ccacgccatg cagctcgccg atgtggatgg cgacggcgtg ctcgatgtgg
    15841 tcacaggcaa gcgccgctgg gcgcacggtc cgctcaagga cgacgagccg aacgcgccac
    15901 ccgtgctcta ctggttccag atcaagcggg atggcaaagg cggcgcgcaa ttcatccccc
    15961 acctcataga cgatgccagc ggcgtcggca cccaggtcac gccgggcgat gtcaacaagg
    16021 acggcaagat ggatgtcgtc gtcggcaaca agatgggcgt ctttgtcctc ctccaggaat
    16081 aaaccgcgcc catgatgccg ggcagaaaat tgcttctgcc cggcgtattc tggcgcatgt
    16141 tgtccgccat gcgcctgaac gccctccttt ttctcacgtt ctccgcggta gcactcgccg
    16201 ccaaatccgt cgaggagatc gccgccgaag tgaagccctc cgtggtgaaa atctcccagg
    16261 tcgggcgcga ggggttcgat ggcctcggca ccggcttcgt cgtcagcgcg gacggcctca
    16321 tcgccacaaa cctgcacgtc attggcgagg cgcggcagct tgaggtcgag accgccgacg
    16381 ggcgcaagca cgaggtcgtc gaggtcaccg ccaccgactc gcactgggac ctcgccctcc
    16441 tgcgcgtggc cagcaaagac ttgcagccac tgcccctcgg tgacaactcc accatccagc
    16501 agggccagcc catcgtcgcc atgggcaatc cccagggcct cgccttcagc gtcgtggacg
    16561 gcgtcgtctc cgcctatcca gacctcatag acgacatccc catgatccgc ctcgccgtgc
    16621 ccattgagaa aggcaacagc ggcggccccc tgctcgaccg cgaagggcgc gtgctcggca
    16681 tcctcaccct caaatccgcc cgcaccgaaa acctcggctt cgccatgccc gtcaacgagc
    16741 tcaagcgcat gatcgaatcc cccaaccccg tgcccatgcg gcgctggctc accatcggcg
    16801 tgctcaaccc caaactctgg cagcctctct tcggctcccg ctggacccag cgcgccggca
    16861 tcatccaggc cgccacaccc ggcagcggct tcggcgggcg ctcgctttgt ttgtggcagg
    16921 ccgacacacc gcccgaggtt tttgaaacct ccgtcaacgt caaactcgac agcgaatccg
    16981 gcgccgccgg cctcgtcttc tgcgccgacg gcggcgaccg ccactacggc ttctacccct
    17041 ccggcggaaa gctccgcctc acccgcttcg agggcgccga cgtctatgcc tggaccatcc
    17101 tcgccgatgt ccccgccgag gcctaccgcc ccggcgagtg gaatcacctc cgcgtccgtg
    17161 tggatcagga aaaaatcacc tgctgggtca acggccaggt catcctcacc caggaggaca
    17221 ccggcctgcg cggcggccgc gccggcctgt gcaagttccg caacaccgtc gccgaattcc
    17281 gccagttccg cgtcggcacc gaccttgccg acaagcccct cccccccgca gtcgccggca
    17341 aagtcagcgc cgccctcgaa gccttcgccc aaagccccgc cgcgcgggag gacacgctcg
    17401 ccaccctcct cgaccagccc gccgccagcc gccgcctgct cctcgagcgc cgccgcgagc
    17461 tggagcgcca g
//
LOCUS       DAOMYJ010000094        17418 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Prosthecobacter sp. isolate AMR_MDS_5629
            NODE_1125_length_17418_cov_22.142026, whole genome shotgun
            sequence.
ACCESSION   DAOMYJ010000094 DAOMYJ010000000
VERSION     DAOMYJ010000094.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867850
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prosthecobacter sp. (wastewater metagenome)
  ORGANISM  Prosthecobacter sp.
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Verrucomicrobiaceae; Prosthecobacter.
REFERENCE   1  (bases 1 to 17418)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17418)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.30
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:34:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,742
            CDSs (total)                      :: 4,684
            Genes (coding)                    :: 4,676
            CDSs (with protein)               :: 4,676
            Genes (RNA)                       :: 58
            tRNAs                             :: 55
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17418
                     /organism="Prosthecobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1125_length_17418_cov_22.142026"
                     /isolate="AMR_MDS_5629"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1965333"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(143..1891)
                     /locus_tag="PLP58_11280"
     CDS             complement(143..1891)
                     /locus_tag="PLP58_11280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ09145.1"
                     /translation="MPKINTDNNPSLEDISASLGNHNATALRGGDHSISAKTGVTARL
                     GSLWDRVSGRAEDKAQKAEAALASAFKKSTGEFDPTDEQKRMISDIVRGRHPGGVKAG
                     LDALQNQALQKKEEIAEIKNSSYPKCKQDIEDTIQQIGQCKNSREVDQSLTKLAQLQG
                     QAKAMHGKLKHESPGPVNVADQKNQALAQVEQNASLQGVEKSASVALASMAINCVDDK
                     GTEDFINSIKEDAGLDAGQQNQVLCKVASHSAKVACLSSQDMGNLARGNDPAEKFLSN
                     ITKMELQDVSKAVADKVVEKSQSVNASQLKGGHDRMPGAGTPESQNTPGLEQAYKELA
                     VSGLQQIVAESEKVKPEGKAILNAIKQGGVEAAGIKPELLNDGATPDDLGNQLVASQV
                     ALRAVSPEISKRAIALRSSADPGARAQGEMLVQTAVLIQGYDNCQRQETPEKFATGEW
                     AKAQNSTINDIREENPQLLAEYRAAGSKYTAPNQAQNQVVDHSQQQSQSQDVEVKEDV
                     GVQADIEVNEEKKQKVGQAANIVHVGGGADGKKVESPALEEGEVKVKEIVKPPQPDED
                     PSKLSFQQKRAIFSKK"
     gene            complement(2001..3149)
                     /locus_tag="PLP58_11285"
     CDS             complement(2001..3149)
                     /locus_tag="PLP58_11285"
                     /inference="COORDINATES: protein motif:HMM:NF012652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase, T2SS/T4P/T4SS family"
                     /protein_id="HRJ09146.1"
                     /translation="MSFTVDNLLGRLADSYPDREISDVQLRTGGLIYLHTNRGLEIVE
                     SLGPADADSVTAVAAALYSQQTVEIWDDPEAVGGVEKMWALLRSKRVIDFSCDQGALG
                     SPVRMRVQVHLSDHGMGITCRCLRSRITQLESLGLDPMITDSLRELMQRRFGLGLVTG
                     PTGSGKSTTLAAILDWVRRNFQKHIVTVEDPIEYRYDTQMEDPNRPGIMLPCPSLVTQ
                     QEVGRHTQSYQSGLKEVLRKTPNIILLGEIRDRETMETCIEAAQTGHFVISTLHTRGA
                     VKTIDRILEFFPKEQQHAILHRLGETLTFVLSQGLLTGFNGRVLVTEYLQNTNEAVSS
                     GIRAYDGSATSLEDALRCKGNQRWNQSLMSLYRAGRISEEVFNSNTLG"
     gene            complement(3163..5103)
                     /locus_tag="PLP58_11290"
     CDS             complement(3163..5103)
                     /locus_tag="PLP58_11290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ09147.1"
                     /translation="MRSTKRTSGYAALPAIAGIAMMLTLSLTMLFKQALVTRDQAAKT
                     QLKLDYSQREEALMRALVAVFPSRAIACMKADHAAGENWSWSEIFADAVALSGADLRL
                     TEDMVKDLRLETARQADVGDGSGAEVRSWITSLTGTAGRVTPGTTAYADVFSQSAFTG
                     RVPPLLEMSQSLQEADALRPVVTPAKRYAAQSAGLLADVTAHPVYNLIPYPNIRFGYA
                     QPGQPFVAKRNWWAFSVNYGGARQPVVRHYVLSLYEVPSQMPIEAATFAAIGQHEDGA
                     FWSMERVRIEGSVYADAMAVESAHGASRLAGRSGIEMSGPLDLGGVEVDSNFDALGVR
                     EQLQAERQSDALPVALSANSGRLAFLPLQPGNSFLLRPSAGAPSAWDDYLAGAGRCRV
                     TVEALEMISLADQTPVKLRVRFQNTAGGVSEAVLQRGVNWPTIFDEGGEAMPFQTELT
                     DNGRSCLTFKPALFDAWLLAGNGAGIATNNSLWFGVDTDAAPESIKPPDDPPAPDDMC
                     VIIRQGKDLTAWTSGISVVTPHRVYIGDDLNAVPAAQPPAGSGLADTDEFFPPLSIFS
                     AELRIGTTVVNRLVEHHGQLGTLSKTGPSAWKPLDIKLGSDDAVHSDHISADLKPLRS
                     PAELPPVHQMNWLVVIEEITQD"
     gene            complement(5100..5633)
                     /locus_tag="PLP58_11295"
     CDS             complement(5100..5633)
                     /locus_tag="PLP58_11295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ09148.1"
                     /translation="MKIRAATSRLTRRAGFTLVEVSTAMGLMLVLGSSMVVMIQQHVT
                     FMRFVQQQNFLAEEAPRTGRLIGRLFDQADHYLIYDDLDAALAGAPPVIFDGRAARLF
                     FKSAAQTVESRVIYVESGGTGALLRFHHTHPDGSDASWTISDRIQDATFAADQGILTL
                     TLHGPNGEEITYGGGAR"
     gene            complement(5630..6097)
                     /locus_tag="PLP58_11300"
     CDS             complement(5630..6097)
                     /locus_tag="PLP58_11300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ09149.1"
                     /translation="MNPPRTLHRARKHQGSVLIEVSLGLGVATVLALMLMKASLLSVG
                     SNQWTIMQTLTDAFMTRETALANRIPVADLTAPDSIWPDAGEDDPPRHEETVTLGRVA
                     GGVEITGDLIRFRVNETGGEEDAPLAIWRLHSILTYHIGGKQYVKSRATLRTL"
     gene            complement(6113..7192)
                     /locus_tag="PLP58_11305"
     CDS             complement(6113..7192)
                     /locus_tag="PLP58_11305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ09150.1"
                     /translation="MKQLAQLLTAIIFAWAQLPAQESWVRQTDLTSGLTYDLPLGPTG
                     GPFTAALPVSEQGSLFELFARGTAWDNNIYLLDTKLIRAYAPVIELVIATEDAYVRGD
                     PASSNFVRRTRADRPFGINLQVSGLVPGSTNQAERELYFSIQGRLFDTETYSGAGAEA
                     FLIHEANLQNGAQSLSPLYHELPSASLSGGCGEQIYTFIRYAADGVPDTILAQPKIEV
                     WPVATASIENVTAGQVFLDRIPSIIVRLNHLYPDSRTYVQLYTGTQALGTAGTLINGT
                     ERRFGRHYNPELEEEPTNVPQTLSISMEDLSNYAATDGIYTLEVITETPFFNRAPERL
                     LHVTFEVDRTVSSRGQITTAERSPE"
     gene            complement(7205..7759)
                     /locus_tag="PLP58_11310"
     CDS             complement(7205..7759)
                     /locus_tag="PLP58_11310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ09151.1"
                     /translation="MKLPIPTRSAVFMTGLLLATAAAAPAQTEPLMKRPVPRDVVTEE
                     ALRQEAAKTRKTSDWLLETTKPVTGRRPAAQTSPYARSIILFDGEKHTLVPFGAILHL
                     PESLQNRVISEPAGDFTFWPSFLKRNTAWLHAREVTLAMAKGDAKSAAPLLRELTGER
                     RLVVSVYRGGPISILEPAPGAGKP"
     gene            complement(7765..8340)
                     /locus_tag="PLP58_11315"
     CDS             complement(7765..8340)
                     /locus_tag="PLP58_11315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ09152.1"
                     /translation="MKTTWLLTLVLGTATCAHSDIILGAPGVPVGARIAVQVPFKINY
                     QLTNSYKFVLSGIQAKGDTLGAPGHSGTKGNGGQFHLKANVWDNNQTPREHVGNLYFR
                     DVQEFNTAPLQTLLVTPAAVFKATSYPITVTTEWQGNGNAHKPNGFFNLLGEYQKNPP
                     VPVGSLHFDVGDWLLVSAGTQPDGVATITRD"
     gene            8609..9304
                     /locus_tag="PLP58_11320"
     CDS             8609..9304
                     /locus_tag="PLP58_11320"
                     /inference="COORDINATES: protein motif:HMM:NF018178.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1080 domain-containing protein"
                     /protein_id="HRJ09153.1"
                     /translation="MPSPDMRAETPPEGFRALFNGKDLTGWHGLNPHSVVKLEGEKKA
                     AALQKMRDEFTAHWRVENGELVNDGTGPYATTDEELGDIELLIEYKTVPKADSGIYLR
                     GTPQVQIWDKNQVFDPAKPTRRPHLGSGGLFNNTPDTPGRDPLVLADREFGEWNSFRI
                     RQIGARTWVWLNDKLVVDGAVMENFWDREKPLPAKGPLMLQTHGGEIRWRNVFVREIS
                     AVDAARIQAGGQK"
     gene            9398..10786
                     /locus_tag="PLP58_11325"
     CDS             9398..10786
                     /locus_tag="PLP58_11325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ09154.1"
                     /translation="MKPTALLLSALAFSAHAAAPLDLGTRWELFVDEYLVAHKDGLSL
                     KLHEPMKREIVLTTDKPWEGPTCAYFSVIQDGEKVLMYYRGSSGGSDHSDAQVTCVAE
                     STDGIHFTRPKLGLIEAGGTQDNNVIWRGVESHNFSPFLDTNPAARPGQRFKALGGVK
                     QPGKNWHQGETPGGLYAFASADGIQWTKMRDTPVITKGAFDSQNLAFFDTTRDRYACF
                     SRIFLNKVRAVQSHHSADFLTWSDGVPHEYAEGVPWEHFYTSATVPCPTAPHLFLSFP
                     KRFMTARHKVPEHKDKGVSDAVFMSSRDGVHWDRPFLEAWVRPGPDLKNWTDRNNMTA
                     NGIVITGEGEWSLYISEHYRHPDHRIRRLTVRKQGFASMHVGAQGGSFTTKPLKVTGS
                     RLLLNYATSAAGSVQIEALDESGAVAAKSAEIYGDEFEAPLLDLSKLVGKNITLRVSM
                     KDADIYALQFAD"
     gene            10799..11854
                     /locus_tag="PLP58_11330"
     CDS             10799..11854
                     /locus_tag="PLP58_11330"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialidase family protein"
                     /protein_id="HRJ09155.1"
                     /translation="MKHLLCLLALCAPLAAQEITRFIAIDNVCAWPNLTLLPDGSINA
                     TIFGQPSHGQIAGAAECWNSKDGQFWEKRGIPAPNDPNTNRMNVAAGLAKNGDLLVLC
                     SGWTNEKQPQRPKQPDFRDDILSSWVCRSSDGGRTWSQIKDFPAPDAGWTNYIPFGDI
                     KVGEDGALHVSCYGGEFTDPTKSTKTKGYRSWHFRSDDDGKTWQRTGVIGEKSNETTL
                     LHLGGRRWMAASRYTTMEIYLSEDDGVTWSGPRVVTAKNEINGHLLRLKDGRILMAYG
                     SRVKDQYGVLAKITADEGKTWTEPVRVAHTLMSDCGYPSTVQRADGKLVTAWYSKASE
                     NHQRYHMGAAIWDAPLP"
     gene            12131..12262
                     /locus_tag="PLP58_11335"
     CDS             12131..12262
                     /locus_tag="PLP58_11335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ09156.1"
                     /translation="MTKLAEPGAFQACVCEVNAGIESMGLYYRICGEFILRKLQVAA"
     gene            complement(12268..13566)
                     /locus_tag="PLP58_11340"
     CDS             complement(12268..13566)
                     /locus_tag="PLP58_11340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007415188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-like beta-propeller repeat protein"
                     /protein_id="HRJ09157.1"
                     /translation="MRAALFLLIASALHADDWPQWLGPQRDGVWRETGIVEKFPDGGP
                     NVLWRVKIGGGYTGPAIADGKVYLMDRQVAAKSDVPGNAFARGIIPGTERVLCLDAKT
                     GAQLWEHRYDCTYTMSYSTGPRCTPLVSDGKLWTVGAEGNLFCLDATNGKVLWSHDFK
                     ADFKARTPMWGFAGHPLLDGRRLICLVGGPGSVAMAFDKDSGKELWRALDAREPGYAP
                     PTMIEQGGTKQLILWHPQSVNSLDPATGKVLWSHSWEIRAGLTIATPRLAGDFLLLSS
                     FYTSSKCFRPDGTLVWEGNSFSEKNTDTLHSLMSTPFVENGHIYGVCSYGQLRCLKLE
                     TGERVWETMAATTGGPLVRWANAFIVKHEDRFFLANELGDLIIAKMTPQGYEEISRAR
                     ILKPTTTDPRRAVVWSHPAFANQCVFMRNDEEIVCVSLAK"
     gene            complement(13628..>17418)
                     /locus_tag="PLP58_11345"
     CDS             complement(13628..>17418)
                     /locus_tag="PLP58_11345"
                     /inference="COORDINATES: protein motif:HMM:NF024298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="WD40 repeat domain-containing protein"
                     /protein_id="HRJ09158.1"
                     /translation="YAAEMRTAGNAAADPAGHARLAGLVEKWRPGNGFAPAAKRGWEW
                     HLLSSLAQEVQILTIPCPGAVKSLDWSPDGKTIHSAGYAAADSWDSSTGKKLQRHSSG
                     REARWLLASPDGLHLALVSDGRLSILPRQGGPALKEWPEAPGSVTRIAWHPAGAILAV
                     GHAGSAGKLMLWHWQMEREPDQIAVPGGWGGFVWSPSGDRLVLVRGNQPPLVIFPGEA
                     ERRMTMETPPDADITCAAWSPDGKHIAGGTRAGEVLLWDAASGKSVHKLNSPGFPVLC
                     LEWSRQGALAAGDEGGLMSVWKEPAAPGEAPLVHKGHRLGIHQVKWSQDGTRLASCSE
                     DKSVRIWPGDGSGAFQMTKTLEFLSARPQWSPDGSLLALPDRETLTLAAPDASGGWKA
                     RTKLPFKIGAVSWSPDGSRLAAVNEAPGVVMLLDAAELRETRRIQSPTPGVSTAEWSP
                     DGTMLATAGRWPKSSGLRIWSAQTGELLWEPAKAGEGAHLFQKLAWHPEGRILAAQPG
                     IGPVSFFEFDPETRTLKRFAENRDAGVWASPALRWKPDGSALLTAGLERTLDLRDPSG
                     SGMMLSLRGHTARVQGFDWSPDGSRIVSIGQDRILRWWDALSGDELLSLPLPGPLPGH
                     VSWSPDGSRILVTDQDGQARMLDALPGMISHPAADCLPALVARGEEAELKLFSFEDFD
                     PEIAWRILKVLETAPTQSQWTAASQHSRAMRFVTTLAREAPDAAAALLKPHMDSALLQ
                     LGLARSEALRGEWAAAAAHYQRAMPEAGRFPEMLYEAAIAARLAGDEALFDASFEKLL
                     AHSSNQPNNEQIACTLARTAAVAGAEERLDKDFARNLAARLLAGAPRWFKSTGALLSH
                     RLGLPESILLLAAHPTPNPATRIEPLKRALATPASAEEHLQEAEAWAASLPLDPRGGI
                     ASSRVHPTDWLEFAALWKELRTGRENPDLLPQRALLPLLATVETNHREEAARVLHAAL
                     SGGHDLSALAPESPVTGKAGILAWQQRFSPEGSTSDPGAAAARWHELRADAAEKRGNW
                     RGSAASWAAAAKAAGTAGRHVRARNAFLISKQWSEAADHHLAALAFEPEDAQLLNEAP
                     AIIRLGQGAPAHREWMARHGARLEAEGDPRSSAMRLMREALSPGTPPADTFARVRGHT
                     QAGNPSPRELPFRLMTLALASLRAGDPAAAVSFLDQDFALFADSDTVPMLHGSIRALI
                     LSHLGETAAANQTLKKVSADLDSGFGRMAARGRPGTRDWHHYIIAECLRLEAEEVLRR
                     "
BASE COUNT         2969 a         5366 c         5730 g         3353 t
ORIGIN      
        1 gctcccgtcc gctcattttt tgctgaatat ggcgcgtttt tgctggaacg acaataaacg
       61 cgcgcgctcc cgtccgctca ttttttgctg aatatggcgc gtttttgctg gaacgacaat
      121 aaacgcgcgc gctcccgtcc gctcattttt tgctgaatat ggcgcgtttt tgctggaacg
      181 acaattttga gggatcctcg tccggctgag gtggtttgac gatttctttg accttcactt
      241 ccccttcctc cagggcgggc gattcgactt tcttgccgtc cgccccaccg cctacatgga
      301 caatgtttgc tgcctggccg accttttgtt ttttctcctc gttgacctcg atgtccgcct
      361 ggactcccac gtcctccttg acctcgacgt cctgggactg gctctgctgt tgcgagtggt
      421 ccacgacctg gttctgcgct tgattgggcg cggtgtattt cgaaccagcg gccctgtact
      481 ctgccagcag ttgagggttt tcttcacgga tgtcgttgat ggtggagttt tgggctttgg
      541 cccactctcc agtggcaaat ttctcggggg tttcctggcg ctggcaattg tcatagccct
      601 ggatcagcac cgccgtttga acgagcattt cgccctgcgc ccgcgcgcca gggtcggcac
      661 tgctccgaag agcgatcgcg cgtttggaaa tttccggtga taccgcgcgc agcgccactt
      721 gggatgcgac gagctggttg ccgaggtcgt cgggagtggc tccgtcgttc agcagttcgg
      781 gcttgatccc ggcggcttca acacctccct gcttgatggc attgaggatg gctttccctt
      841 cgggtttcac cttctcggac tcggcgacta tttgctgcaa gcctgagact gccagttctt
      901 tgtaggcctg ctcaagtccg ggggtgtttt gggattcagg cgttcccgcg ccgggcatcc
      961 ggtcatgccc gcccttgagc tgggaggcgt tcacactttg agatttttcg accaccttgt
     1021 ccgccaccgc cttgctgaca tcctgcaact ccattttggt gatgttggag aggaattttt
     1081 ccgccggatc gttgccccgc gccaggtttc ccatgtcttg cgagctgagg caggcgacct
     1141 tcgcggagtg gctggccacc ttgcacagga cctggttttg ctgcccggca tccaggccgg
     1201 catcctcctt gatgctgttg atgaagtcct cggtgccttt gtcgtccacg cagttgatgg
     1261 ccatcgaggc cagcgcgact gaggcgcttt tctcgacgcc ctgaagactg gcattctgct
     1321 cgacctgggc gagcgcttga tttttctggt cagcgacatt gaccggcccg ggcgactcgt
     1381 gtttgagctt gccatgcatg gctttggctt ggccctgaag ttgggcgagc ttggtcaggg
     1441 attgatccac ctcccggctg ttcttgcact ggccgatctg ctggatcgtg tcctcaatgt
     1501 cctgtttgca cttgggatac gaggagtttt tgatttcagc gatttcctcc ttcttttgca
     1561 gggcctgatt ttgcagcgcg tccaggccgg ctttcactcc gcccggatgc ctgccgcgca
     1621 cgatgtccga gatcattcgt ttttgctcgt cggtcgggtc gaactcgccg gtggattttt
     1681 tgaatgcgct ggcgagcgcg gcctcggctt tttgcgcctt gtcctcggcg cgcccgctga
     1741 cgcggtccca tagcgagccg aggcgcgcgg tgacgccggt cttggcgctg atggagtggt
     1801 cgccgccgcg cagagcggtg gcattgtggt tgcccaggct tgcgctgatg tcctccaggc
     1861 tcgggttgtt gtcggtgttg atcttgggca tggcgtgcgg tgtggagttg tggcgctgtc
     1921 gtgttattcg cggcgccatc ccttgttagc ctgatccgcg tctgctgtga cagacttccg
     1981 tccgcgcgcg cggcccgcgt ctagccgagg gtgttggagt tgaagacctc ctcgctgatg
     2041 cgcccggcgc ggtagaggga catgagggac tggttccagc gctggttgcc tttgcagcgc
     2101 agggcgtcct ccaaggaggt ggcgctgccg tcgtaggcgc ggatgcctga ggagacggcc
     2161 tcgttggtgt tttgcaggta ctcggtgacg agcacgcgcc cgttgaagcc ggttagcagg
     2221 ccctgggaga ggacaaaggt gagtgtctcg cccaggcggt ggagaatggc gtgctgctgc
     2281 tccttgggga agaactcgag gatgcggtca atggtcttca ccgcgccgcg ggtgtgcagg
     2341 gtggagatga cgaagtggcc ggtctgcgcg gcctcgatgc aggtttccat ggtctcgcgg
     2401 tcgcggattt ccccgagcag gatgatgttg ggcgttttgc gcagcacttc tttgagcccg
     2461 ctctggtagg actgcgtgtg gcggcccact tcctgctggg tgacgagcga agggcagggg
     2521 agcatgatgc cgggccggtt cggatcctcc atttgtgtgt cgtagcggta ttcgatgggg
     2581 tcttcgactg tgacgatgtg tttctgaaag ttcctccgca cccagtcgag gatggcggcg
     2641 agtgtggtgg acttcccgga cccggtggga ccggtgacaa ggccgaggcc gaagcggcgc
     2701 tgcatgagct cgcgcaggct gtcggtgatc attgggtcga ggccgaggct ttcgagctgg
     2761 gtgatgcggc tgcgcaggca gcggcaggtg atgcccatgc cgtggtcgct gaggtggacc
     2821 tgcacgcgca tgcgcaccgg ggagccgagc gcgccctggt cgcaactgaa gtcaatgacg
     2881 cgctttgagc gcagcagcgc ccacattttc tccacgccgc cgacggcttc cgggtcgtcc
     2941 cagatttcga cggtctgctg cgagtaaagc gccgcggcca cggctgtgac gctgtccgcg
     3001 tccgcggggc cgagcgattc gacgatctcc aggccgcggt tggtgtgcag gtagatgagg
     3061 ccgccggtgc gcaactgcac gtcggagatt tcccggtcgg gataggagtc cgccagccgg
     3121 cccaggaggt tgtcaacggt gaagctcatg gcgcggaggg gttcaatcct gggtgatctc
     3181 ctcgatgacg acgagccagt tcatctgatg cacgggcggc agctcggcgg ggctgcgaag
     3241 cggcttgagg tcggcgctga tatggtcgga atgcacggcg tcatcgctgc caagtttgat
     3301 gtcgagcggc ttccaggcgg atgggccggt cttgctcagg gtgccaagct ggccatggtg
     3361 ctcgacgagg cggttgacca cggtggtgcc gatgcgcagc tcggcggaga atatggacag
     3421 cggcgggaaa aattcatccg tgtctgcgag acccgaccca gcgggtggtt gcgcagccgg
     3481 cacggcgttg aggtcgtcgc cgatgtaaac gcggtgaggt gtgacaacgg aaatgccgct
     3541 ggtccaggcg gtgaggtctt tgccctggcg gatgatgacg cacatgtcat ccggcgcggg
     3601 cgggtcgtcc gggggtttga tggattccgg ggcggcatcc gtgtccacgc cgaaccaaag
     3661 gctgttgttg gtggcgatgc ccgcgccgtt cccggccagc agccatgcgt cgaagagcgc
     3721 gggtttgaag gtcaggcagg agcggccgtt gtcagtcagc tcggtctgga agggcatggc
     3781 ctcgccgccc tcgtcaaaaa tggtgggcca gttgaccccc cgctgtaaaa cggcctcgga
     3841 gactccgccg gcggtgtttt gaaaacgcac ccgcagcttc accggtgtct ggtccgccag
     3901 gctgatcatc tcgagggctt ccacggtgac gcggcagcgg cccgcgccgg cgaggtagtc
     3961 gtcccacgcg cttggcgcgc ctgcggaggg gcgcagcaga aaggagttgc cgggttgcag
     4021 cgggaggaag gcgagccgtc cggaattggc ggacagggcc acgggcagag catcgctttg
     4081 cctttcggcc tggagctgct cgcgcacgcc gagggcgtcg aagttggaat ccacttcgac
     4141 gccgccaagg tccagcggcc cgctcatctc gatgccgctg cgccccgcca gccgggaggc
     4201 tccgtgggcg gactcgacgg ccatcgcatc cgcatagacg gagccttcga tgcgcactct
     4261 ttccatgctc cagaatgcgc cgtcctcatg ctggccgatg gctgcgaagg tggcggcctc
     4321 gatgggcatc tgcgagggca cctcgtacag ggagagcaca taatgccgca ccaccggctg
     4381 gcgcgcgccg ccgtagttca ccgagaaggc ccaccagttt ctcttggcca caaaaggctg
     4441 gccgggctgc gcgtagccga acctgatgtt tgggtagggg atgagattgt aaaccgggtg
     4501 cgcggtcaca tccgccagca ggcccgccga ttgggcggcg tagcgtttcg cgggcgtcac
     4561 cacggggcgc agggcgtccg cctcctgcag gctctggctc atttccagca agggcggcac
     4621 cctgccggtg aaggcggact gggaaaagac atcggcgtaa gcggtcgtgc ccggggtcac
     4681 ccgcccggcc gttcctgtga gggaggtgat ccagcttcgg acctcggcgc ctgagccgtc
     4741 tcccacgtca gcctggcgcg cggtctcaag ccgcaaatct ttgaccatgt cttctgtgag
     4801 gcgcaagtcc gctccggaga gcgcgacggc gtcggcgaag atttcactcc acgaccagtt
     4861 ctcccccgcc gcgtggtccg ccttcatgca ggcgatggcg cgcgagggga agacggccac
     4921 gagcgcgcgc atgagggcct cctcgcgctg gctgtagtcg agtttgagct gcgtcttcgc
     4981 cgcctggtcg cgggtgacga gggcctgctt gaagagcatg gtcaggctca gggtgagcat
     5041 catggcgatg cccgcgatgg cgggcagggc ggcgtaaccg gaggttcgtt tggtgcttct
     5101 catcgcgctc ctcctccata agtgatctcc tcgccgttcg ggccgtgcag ggtgagtgtc
     5161 aggatgccct ggtcggcggc gaaggtggcg tcctggatcc ggtcgctgat ggtccatgag
     5221 gcgtccgagc cgtccggatg cgtgtggtga aaccgcagca gcgcgccggt gccgccgctc
     5281 tccacataaa tgacgcgcga ctccacggtc tgcgcggcgg atttgaaaaa gagacgcgcg
     5341 gccctgccgt caaagatcac aggaggcgcg ccggccagcg cggcgtcgag gtcatcgtaa
     5401 atgagataat ggtccgcctg gtcaaacagc ctgccgatga gcctgccggt gcgcggcgcc
     5461 tcctccgcga ggaagttctg ctgctggacg aaacgcatga aggtaacgtg ctgctggatc
     5521 atcaccacca tgctggagcc gagcacgagc atcagcccca tggcggtgga gacctccacg
     5581 agagtgaagc ctgcgcgtct tgtgaggcgg gatgtagcgg cgcggatttt cataaggtgc
     5641 gcagggtggc gcggctcttc acatactgct tgccgccgat gtggtaggtg aggatggagt
     5701 gcagccgcca gatggcgagc ggggcgtcct cctcgccgcc ggtttcattg acgcgaaatc
     5761 ggatcaggtc cccagtgatc tccacgccgc ccgccacgcg gccgagagtg acggtttcct
     5821 catggcgcgg aggatcgtcc tcgcccgcgt cgggccagat ggagtccggc gctgtgaggt
     5881 ctgccacggg gatgcggttg gccagggcgg tctctcgggt catgaaggcg tccgtcagtg
     5941 tctgcatgat ggtccactgg ttgctgccca cggagagcag ggaggctttc atcagcatga
     6001 gagccagcac ggtggccaca cccaggccga gggagacctc gatcaaaaca ctgccctggt
     6061 gcttgcgggc gcggtggagt gtgcgcggcg gattcatggc atgttgttat tctcactccg
     6121 gcgagcgctc cgccgtggtg atctgtccgc gggatgagac ggtgcggtcc acctcgaagg
     6181 tgacatgcag caggcgctcc ggcgcgcggt tgaaaaaggg cgtctctgtg atgacctcca
     6241 gggtgtagat gccgtcggtg gcggcatagt tcgagaggtc ctccatcgag atggagaggg
     6301 tctggggaac gttggtgggc tcctcctcga gttcggggtt gtaatgacgg ccgaagcgcc
     6361 gctcggtgcc gttgatcagc gtgcccgccg tgcccagcgc ctgagtgcct gtgtaaagct
     6421 gcacataagt ccgcgagtcg ggataaaggt ggttcaggcg gacgatgatg gaggggatgc
     6481 ggtcaaggaa gacctgcccg gcggtgacat tctcaatgct ggcggtggcg accggccaga
     6541 cctcgatctt cggctgcgcg aggatggtgt ccggcacgcc atccgccgcg tagcgaatga
     6601 aggtgtaaat ttgctccccg cagcctccgc tgagactggc ggacggcagt tcgtggtaga
     6661 ggggcgagag cgactgcgcg ccgttttgca gattggcctc gtggatgaga aaagcctccg
     6721 cgccagcgcc ggagtaggtc tccgtgtcga acaagcgccc ctgaatggaa aaatacagct
     6781 cgcgctccgc ctggttggtg ctgccaggca ccaggccgga aacctggagg ttgatgccga
     6841 aggggcggtc cgcgcgggtg cggcgcacga agttcgaact ggctgggtcg ccgcgcacat
     6901 aggcgtcctc ggtggcgatg accagttcga tcacaggcgc gtaggctcgg atcagcttgg
     6961 tgtcgagcag gtagatgttg ttgtcccagg ctgtgccgcg ggcgaagagt tcgaagagcg
     7021 aaccttgctc gctgacaggc agggcggccg tgaagggccc gccggtgggt cccaagggca
     7081 ggtcgtatgt caggccgctg gtcaggtccg tctgccggac ccatgactcc tgggcgggga
     7141 gctgagccca ggcaaaaatg atggcggtca ggagttgcgc gagttgtttc atgtcgtcaa
     7201 aggctcaggg ttttccggct ccgggcgcgg gttccagaat gctgatcgga ccgccgcgat
     7261 agacggagac gaccagacgg cgttctcctg ttagctcgcg cagcaggggc gctgccgact
     7321 tggcatctcc cttcgccatg gccagggtca cttccctggc gtggagccag gccgtgttgc
     7381 gcttcagaaa tgagggccag aatgtgaagt cgccggcggg ttcggagatc acccggttct
     7441 ggagggactc cggcaaatgc aggatggctc cgaacggcac cagcgtgtgc ttttcaccgt
     7501 caaagaggat gatggaccgg gcataggggg aggtttgcgc ggcggggcgc ctgccggtga
     7561 ccggtttcgt tgtctcaagc agccagtcgc tggtttttcg cgtttttgcc gcctcctgcc
     7621 tcagcgcctc ctcggtcacc acatcgcgcg gcaccgggcg cttcatcagc ggctctgtct
     7681 gcgccggggc cgcggcggct gtggcaagga gcaggcccgt catgaagacg gcgctccgcg
     7741 tcgggatcgg gagtttcatc ggcgtcagtc tcgtgtgatg gttgccactc catcgggctg
     7801 cgtgccggcg ctgaccagca gccagtcacc cacatcgaag tgcaggctgc cgaccggcac
     7861 gggtggattt ttttggtatt cgccgagcag gttgaagaag ccgttcggtt tgtgggcgtt
     7921 gccattgccc tgccattccg tggtcacggt gatggggtag ctggtcgctt tgaagactgc
     7981 cgccggtgtc accagcaggg tttgcagcgg agccgtgtta aactcctgca catcgcggaa
     8041 gtagagattg ccgacatgct ccctgggcgt ttggttgttg tcccagacat tcgccttgag
     8101 gtgaaactgg ccgccgttgc ccttggtgcc cgaatggccc ggtgcgccca gggtgtcgcc
     8161 cttggcctgg atgccggaga gcacgaattt gtaggagttc gtcagttggt aattaatttt
     8221 gaagggcact tgcacggcga tgcgcgcgcc cactggcacg cccggagcgc caagaatgat
     8281 gtctgagtgg gcacaggtgg ctgtgccgag aaccagggtt aggagccatg ttgttttcat
     8341 gagattctca attttgaata ttttccggga cgggaagcca tttccaggat ttccaagcag
     8401 aatgtatcaa atttgagaaa atgacaatgg aaattatgga taataataaa agatccgcga
     8461 tttcacgcaa tttgctcgcc ttcgcggact ttgcgcagct ttcctttccc caattgaaag
     8521 cccgcattct tgccatcgta gcaaacctgc caaccacgca tcccgcatga aatcaccttt
     8581 cgctttcgct cttcttttac tgtccgcctt gccgtctcct gatatgcgcg ctgagacgcc
     8641 acctgagggt ttccgcgctc ttttcaatgg caaagacctg accggctggc atggcctcaa
     8701 cccccactct gtggtgaagc tggagggtga gaaaaaagcc gccgcgctgc aaaaaatgcg
     8761 ggatgagttt accgcgcact ggcgcgtgga aaatggcgag ctggtcaatg acggcaccgg
     8821 cccctatgcc accacggacg aggagttggg ggacatcgag ttgctcatcg aatacaaaac
     8881 cgtgcccaag gcagacagcg gcatctacct gcgcggcacg ccccaggtgc agatttggga
     8941 caaaaaccag gtcttcgatc ccgccaaacc cacacgccgg ccgcatctcg gttccggcgg
     9001 ccttttcaac aacacgcctg acacgccggg ccgcgatccg ctggtgcttg cggacaggga
     9061 attcggcgag tggaacagct tccgcatccg acagatcggc gcgcgcacct gggtctggct
     9121 caatgacaag ctcgtcgtgg atggcgcggt catggaaaac ttctgggacc gtgaaaagcc
     9181 cctgcccgcc aaaggcccgc tcatgctgca aacccacggc ggcgagatcc gctggcgcaa
     9241 tgttttcgtg cgcgagatca gcgccgttga tgccgcgcgc atccaggctg gcgggcagaa
     9301 gtgagttggc gggtgaggcg cggcttcatc gttccgggaa acagggaggc gaaggaatcg
     9361 tgcaagctcc gggcgaaatt cgcttatccc ttcccgcatg aaaccaaccg ccctcctctt
     9421 gtccgccctc gcattcagcg cccacgccgc cgcgccgctc gatctcggca cccgctggga
     9481 gttgttcgtc gatgaatacc tcgtcgcgca caaagacggc ctctccctca aactgcatga
     9541 gcccatgaag cgcgagatcg tgctcaccac cgacaaaccc tgggaaggcc ccacctgcgc
     9601 ctacttcagc gtcatccagg atggcgaaaa ggtgctcatg tactatcgcg gctcctccgg
     9661 cggcagcgac cacagcgacg cgcaagtgac ctgcgtggcc gagagcacgg acggcatcca
     9721 tttcacacgg ccaaagctcg gcctcatcga ggccggcggc acccaggaca ataacgtcat
     9781 ctggcgcggc gtcgagtcgc acaacttctc cccctttctc gacacgaatc ccgccgccag
     9841 gcccgggcag cgctttaaag cgctcggcgg cgtgaaacag ccgggcaaga actggcatca
     9901 aggcgagacg cccggcggcc tgtatgcctt cgcctccgcc gacggcatcc aatggaccaa
     9961 gatgcgagac acgccggtga tcacgaaggg cgcgttcgat tcgcaaaacc tcgctttctt
    10021 cgacaccact cgtgaccgct atgcctgctt cagccggatt tttctgaaca aggtccgcgc
    10081 cgtgcagagc caccactccg ccgactttct gacctggagc gatggtgtgc cgcatgagta
    10141 tgccgagggt gtgccgtggg agcactttta caccagcgcc accgtgccat gccccaccgc
    10201 gccgcatctc ttcctttcgt ttccgaagcg cttcatgacc gcgcgccaca aggttcccga
    10261 gcacaaggac aaaggcgtca gcgatgccgt cttcatgtcc agccgcgacg gcgtgcattg
    10321 ggaccgccct tttctcgaag cctgggtgcg ccccggtccc gacctcaaaa attggaccga
    10381 ccgcaacaac atgaccgcca acggcatcgt catcaccggc gagggcgagt ggagcctcta
    10441 catcagcgag cactaccgcc accccgacca ccgcatccgc cgccttaccg tgcgcaagca
    10501 gggtttcgcc tccatgcatg tcggcgccca aggcggcagc ttcaccacga agccgcttaa
    10561 agtcactggc tccaggctcc tgcttaatta cgccacctcc gccgccggca gcgtgcagat
    10621 tgaggcgctg gatgaaagcg gagcagtcgc ggccaagtcc gctgaaatct acggtgatga
    10681 gtttgaggct ccgcttcttg atctgtcgaa gctggttggg aaaaacatca cgctccgcgt
    10741 cagcatgaag gatgctgaca tctacgccct ccaatttgca gactaacctc aacccatcat
    10801 gaaacacctc ctctgcctcc tcgccctctg cgcgccgctc gccgcgcagg aaatcacccg
    10861 attcatcgcc atcgacaacg tctgcgcgtg gccaaacctc acactgctgc cggatggcag
    10921 catcaacgcc accatcttcg gccagcccag ccacggacag atcgcgggag ccgccgaatg
    10981 ctggaacagc aaggacggcc agttttggga aaagcgcggc attcccgcgc cgaacgaccc
    11041 aaacacgaac cgcatgaatg tcgccgccgg actggcgaag aacggcgacc tgctcgtgct
    11101 ctgctccggt tggacgaacg aaaaacagcc gcagcggccc aagcagccag atttccgcga
    11161 cgacatcctc tccagctggg tatgccgcag cagtgatggc ggcaggacat ggtcgcagat
    11221 caaggatttc cctgccccgg atgccggctg gacgaattac atcccctttg gcgacatcaa
    11281 agtcggcgag gatggcgcgc tgcacgtctc ctgttatggc ggcgagttca ccgaccccac
    11341 caagagcacc aaaaccaaag gctaccgctc ctggcacttc cgcagcgatg acgacggcaa
    11401 aacctggcag cgcaccggtg tgatcggcga gaagagcaac gagaccaccc tgctccacct
    11461 cggcggcagg cgctggatgg cggcctcccg ctacaccacg atggaaatct atctctccga
    11521 ggacgacggc gtcacctgga gcggcccgcg ggtcgtcacc gccaaaaacg agatcaatgg
    11581 ccacctgcta cgcctcaaag acggccgcat cctcatggcc tacggcagcc gcgtgaagga
    11641 ccagtatggc gtgctcgcca agatcaccgc tgatgaaggg aaaacatgga ccgagccggt
    11701 ccgggtcgcg cacacgctca tgtccgactg cggctacccc agcaccgtgc agcgcgccga
    11761 cgggaaactc gtcaccgcct ggtattccaa agccagcgaa aaccaccagc gctaccacat
    11821 gggcgcagcc atctgggacg cgcccctgcc ttgaaccggg aattgacccc ggcacttccg
    11881 tcttcgcacc tgcggcggca ctgaagatgg cccggcttcc gcaaaagcag ggctttgctc
    11941 acaaagccag atgctggcaa gccaggaagc ccggatgctg aaatccccgg gcgattctcg
    12001 ccatcgtcat cccagccatg agcgcaaccg ccatacccca tcatgattcg ctgcctggcg
    12061 ggccgctggt gatccagacg cagggctgca cgccgcgtct gtgggcgggc cggcgggcgc
    12121 ggattttctc gtgaccaagc tggcggagcc gggagcgttc caggcctgtg tgtgcgaggt
    12181 caatgccggc atcgagtcca tggggcttta ctaccgcatc tgcggggagt tcattctgcg
    12241 aaagctgcag gtcgcagcat aaggcggtta cttcgccagt gacacgcaca cgatctcctc
    12301 gtcgttgcgc atgaagacgc attggttcgc gaaggcggga tgcgaccaaa ccaccgcgcg
    12361 gcgtggatcg gtggtggtag gcttcaagat cctggcccgg ctgatctcct cgtagccttg
    12421 gggagtcatt ttcgcgatga tgaggtcgcc cagttcgttg gcgaggaaga agcggtcctc
    12481 gtgcttcacg atgaaggcgt tcgcccagcg cacgagcggg ccgccggtgg tggcggccat
    12541 cgtttcccag acgcgttcgc cggtttcgag cttcaggcag cggagctggc cgtagctgca
    12601 cactccgtag atgtggccgt tttcgacgaa gggcgtgctc atgaggctgt gcagcgtgtc
    12661 ggtgttcttc tcgctgaagc tgttgccttc ccaaacgagc gtgccatcag ggcggaagca
    12721 tttggagctg gtgtaaaagg agctgagcag caaaaagtct cctgcgagcc gcggcgtggc
    12781 aatggtgagg ccggcgcgga tttcccacga atgcgaccac agcactttgc cggtggccgg
    12841 atcgagcgag ttcacggact gcggatgcca gaggatgagc tgcttcgttc caccctgctc
    12901 gatcattgtc ggcggtgcat agccgggctc gcgggcatcc agcgcgcgcc agagctcctt
    12961 gccggaatcc ttgtcaaaag ccatcgccac gctgccggga ccgccaacga ggcagatcag
    13021 ccgccggcca tcaagcagcg gatgccccgc gaagccccac atcggcgtgc gcgctttgaa
    13081 gtccgccttg aagtcatgcg accacagcac cttgccgttt gtggcgtcca gacagaacag
    13141 gtttccctcg gctccgacag tccacaactt gccatcactc accagtggtg tgcagcgcgg
    13201 tccggtggag tagctcatcg tataggtgca gtcatagcga tgctcccaaa gctgcgcgcc
    13261 ggtcttcgca tcgaggcaaa gcacgcgctc cgtgccggga atgatgccgc gcgcgaaggc
    13321 attgcccggc acatcgctct ttgccgccac ctgccggtcc atgagataca ctttgccatc
    13381 cgcgatggct ggtccggtgt atccgccgcc gattttcacg cgccacagca cgttcggccc
    13441 gccatcggga aatttttcca cgatgcccgt ctcacgccac acaccatcgc gctgcggacc
    13501 cagccactgc ggccagtcgt cggcgtgaag ggcggaggcg atcaggagaa aaagagcggc
    13561 gcgcatgaat ccaacaaact cgccctcgcg catcatgttg cagacgattt tgtgatgcca
    13621 aacttggcta tcttcggaga acttcctccg cctccagtct caggcattcg gcgatgatgt
    13681 aatggtgcca gtcgcgtgtg ccgggccgcc cgcgcgcggc catgcgtcca aagccggagt
    13741 ccaaatcagc gctcactttt ttgagggttt gattcgccgc tgctgtttcg ccgagatggg
    13801 agagaatcag ggcgcggatc gagccatgca gcatcggaac cgtgtccgag tccgcaaata
    13861 aagcgaagtc ctgatccaga aaggagactg cggccgcagg gtcccccgcg cggagactgg
    13921 caagagcgag cgtcatgagg cggaaaggca gctcccttgg agatggattg cccgcctggg
    13981 tgtgcccgcg cactctggca aaggtgtccg ccggtggtgt ccctggagaa agcgcctcgc
    14041 gcatcaggcg catggctgat gagcggggat cgccttctgc ttccagcctg gcaccgtgcc
    14101 tggccatcca ctcgcggtga gctggcgcgc cctggccgag gcggatgatc gcgggtgctt
    14161 cgttgaggag ctgtgcgtcc tcaggctcaa acgcgagcgc tgcgagatgg tgatcggctg
    14221 cctcactcca ttgcttggag atgaggaacg cgttgcgcgc ccggacatgg cgcccggcag
    14281 tgcccgccgc tttcgccgca gcggcccagg aggcggcgga tccccgccag ttgccgcgtt
    14341 tttccgctgc gtctgcgcgc agctcatgcc agcgggctgc ggcggcgcca ggatcggatg
    14401 ttgagccctc gggtgaaaag cgctgctgcc aggcgaggat gcctgctttc ccagtgacgg
    14461 gagattcggg cgccagggcg gagaggtcgt gcccgccaga aagtgccgcg tgcaggacgc
    14521 gggcagcttc ctcccggtgg ttcgtttcaa cggtggcgag cagtggcagg agcgcgcgct
    14581 gggggagcag atccggattt tcacgcccgg tgcggagttc cttccaaagg gcggcaaact
    14641 ccagccagtc ggtgggatgc accctgctgc tcgcgatgcc gccacgcggg tccaagggaa
    14701 ggctggcggc ccaggcttcc gcttcctgga ggtgctcctc cgccgaggcc ggagttgcga
    14761 gagcgcgttt gagcggctcg atgcgggtgg cgggatttgg cgtgggatgc gccgctagaa
    14821 ggagaattga ttctggcagg ccgagacggt gggagagcag cgcaccggtg ctcttgaacc
    14881 agcgcggtgc gcccgcaagc aaacgcgccg ccaggttgcg ggcgaaatct ttgtccagcc
    14941 gctcctccgc gccggccacc gccgccgttc gtgcgagcgt gcaggcgatc tgctcgttgt
    15001 tgggctgatt cgagctgtgt gcgagcagct tctcgaagga ggcatcaaaa agcgcctcgt
    15061 ctcccgccag tcgggcggcg atggcggctt catagagcat ctcgggaaag cgcccggctt
    15121 caggcatcgc gcgttgataa tgcgccgccg cagccgccca ttcgccgcgc agcgcctcgc
    15181 tgcgcgcgag tcccagctgc agcaaggctg agtccatgtg cggcttcagc aacgcggccg
    15241 ccgcgtccgg cgcttcacgg gccagggtgg tgacgaaacg catggcgcgg ctgtgctggc
    15301 tggcggcggt ccactgggat tgggtgggcg ccgtttccag gaccttgagg atgcgccagg
    15361 cgatttcggg atcgaagtct tcgaaggaga acagtttcag ctccgcctcc tcaccccgcg
    15421 ccaccagcgc gggcaggcag tccgcagcgg ggtgggagat catgccgggc agcgcgtcaa
    15481 gcatccgggc ctgcccgtct tgatccgtga caaggatgcg gctgccatcc ggactccatg
    15541 acacgtggcc cggcagcggt ccgggcagcg gcagggagag gagttcgtcc ccggagagcg
    15601 catcccacca acggaggatg cgatcctgtc cgatggagac gatgcggctg ccatccgggc
    15661 tccagtcaaa gccctgcacg cgcgcggtgt gccctctcag ggaaagcatc atgccgctgc
    15721 ccgaggggtc gcgcaggtcg agggtgcgct ccagccctgc ggtgagcagg gcgctgccat
    15781 cgggcttcca gcgcagcgca ggcgaggccc agacgcccgc gtcgcggttc tctgcgaagc
    15841 gtttcaacgt gcgtgtttcg gggtcgaatt cgaagaatga caccggtccg atccctggct
    15901 gggcggccag gatgcgtcct tcgggatgcc aggcgagttt ttgaaacaaa tgcgctcctt
    15961 ccccggcctt ggcgggctcc cagagcagct ctcccgtttg tgcggaccag atgcgaagac
    16021 ccgaggattt gggccagcgg cccgccgtgg cgagcatcgt tccgtccggg ctccactcgg
    16081 cagtgctgac acctggagtg ggtgactgga tgcgccgggt ttcgcgcagc tccgccgcat
    16141 ccagcagcat caccacaccc ggagcctcat tcacggcggc cagccggctg ccgtcggggg
    16201 accatgagac ggctccaatc ttaaagggca atttcgtcct cgctttccac ccgccggacg
    16261 catccggggc tgccaaagtc agtgtctcgc gatccggcag cgccagcagc gagccgtccg
    16321 gactccactg cggacgtgcg gagagaaatt cgagggtctt cgtcatctgg aaggctccgc
    16381 tgccgtcgcc aggccagatg cggacgcttt tgtcctccga gcatgaggcc aggcgggtgc
    16441 cgtcctgcga ccatttgacc tgatggatcc ccaggcgatg gcccttgtga accaggggcg
    16501 cctcaccggg agcggcaggc tctttccaaa cagacatgag gccgccctcg tcaccggcgg
    16561 ccagcgcacc ctggcgggac cattcgaggc agagcacggg aaagcctggg gaattcaatt
    16621 tgtggacgct tttcccggaa gccgcgtccc agagaagcac ctcgcccgcc cgcgtgcctc
    16681 cggcaatatg cttcccgtcc ggactccatg cggcgcatgt gatgtcggcg tcaggaggtg
    16741 tttccattgt catccggcgc tcagcttctc caggaaagat gaccagtggt ggctggtttc
    16801 cgcgcacgag cacgagacgg tcgccggagg ggctccagac aaatccaccc cagccgccgg
    16861 gcacggctat ctgatccggt tcacgctcca tttgccaatg ccacagcatc agtttgccgg
    16921 cgctccctgc atgaccgacg gcgagaatgg cgcccgctgg atgccaggca atgcgggtca
    16981 cgctgccggg agcttccggc cactccttca atgcagggcc gccttgccgc ggcaggatgg
    17041 acagccttcc gtcgctgacc agggccaggt gcagcccgtc gggggaggcg agcagccagc
    17101 gggcttcgcg gcctgagctg tggcgctgga gcttttttcc ggtggaggaa tcccaggagt
    17161 cggcggctgc ataaccggct gaatggatgg ttttcccatc cggagaccag tcgagcgatt
    17221 tcaccgcgcc ggggcatggg atcgtcagaa tctgtacctc ctgcgcgagg ctggagagca
    17281 aatgccactc ccagccgcgc ttcgcggcgg gtgcgaagcc gtttcccgga cgccacttct
    17341 ccaccagccc ggccagccgc gcatggcccg ccgggtcggc agccgcgttg cccgccgtgc
    17401 gcatctccgc cgcataga
//
LOCUS       DAOMYJ010000095        17412 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Prosthecobacter sp. isolate AMR_MDS_5629
            NODE_1126_length_17412_cov_20.911102, whole genome shotgun
            sequence.
ACCESSION   DAOMYJ010000095 DAOMYJ010000000
VERSION     DAOMYJ010000095.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867850
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prosthecobacter sp. (wastewater metagenome)
  ORGANISM  Prosthecobacter sp.
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Verrucomicrobiaceae; Prosthecobacter.
REFERENCE   1  (bases 1 to 17412)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17412)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.30
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:34:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,742
            CDSs (total)                      :: 4,684
            Genes (coding)                    :: 4,676
            CDSs (with protein)               :: 4,676
            Genes (RNA)                       :: 58
            tRNAs                             :: 55
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17412
                     /organism="Prosthecobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1126_length_17412_cov_20.911102"
                     /isolate="AMR_MDS_5629"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1965333"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1695)
                     /locus_tag="PLP58_11350"
     CDS             complement(<1..1695)
                     /locus_tag="PLP58_11350"
                     /inference="COORDINATES: protein motif:HMM:NF019262.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ09159.1"
                     /translation="MRPIHTLFAFTLLAGSLQAQPASADPAMKAAADKVNASGALLMP
                     VAADGKAWRFTALNVAKDFTDAGLEPLIPVADKIASLDLARTKITDAGLKAVAAMKNL
                     AELHLENTGIGDAGLDHLKDLASLEYLNLYNTKVTDAGLQKLAGLAKLKALYLWQSGV
                     TKAGVAQLKARLPGAHINTGWTAEDDAKPAPFIAATPAADAKPAADAPKPAAPAPAKP
                     EEKKADAPKPAAAAKLDPALAAKAVVYRDVIAPIMAAKCVGCHGEEKKKGKLQLHDYA
                     SILKGGSEGENNVVAGKPAESLMLIRINLPADDDEHMPPEDEPQISKEELAVLKWWIE
                     TGASETATVAAAKPTPDAEAALALLLAKGLPKQDAKTTAAPAKSKPAPLTDAEKKQIT
                     EITAKIQALNATLMPLAQDTEQLRLSVINATDKFGDKELALLEPIAKHLVWVDLARSQ
                     VTDAAADTLAKMTALERLHLENTKVTDAGLAKLAALQTLEYLNLYGTKTTDAGISKLA
                     GAKGLKKLFVWQTGVTKAGAKALEGQLPGLVVNVGLSEAEIAKLTSTPPAAPPPPPA"
     gene            complement(1721..2797)
                     /locus_tag="PLP58_11355"
     CDS             complement(1721..2797)
                     /locus_tag="PLP58_11355"
                     /inference="COORDINATES: protein motif:HMM:NF019262.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ09160.1"
                     /translation="MSSFDSSSKPRMGTWALALLIILGYVTVLIAFPPRFEPVEGETS
                     SLLLFIGRFHPIFLHLPVGALGLLCLMELGCLTRSGEEKLGGAALLTLLVGSAGAVMA
                     VLAGILLSREGGYAGGNFSLHQTLAIIGTSGILLALVVRMLGMGQEERGLLHFYRALF
                     FASFGLMGLGAHFGGNMSHGSKYLTAHAPEPLKSRMIAMENWMLGFVEKPKAELETPD
                     TLPPAETPPVGATSSDKLVFQHVLMPIFEAKCNTCHNEDKTKGGLRMDTYALLMQGGD
                     SGESVIPGKPDESLAIERILLPEDDDEHMPPKGKEQMTADELALFRWWIEQGASETLK
                     MDEAKLPENLKTAAEAVMKGQTAS"
     gene            complement(2879..3274)
                     /locus_tag="PLP58_11360"
     CDS             complement(2879..3274)
                     /locus_tag="PLP58_11360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ09161.1"
                     /translation="MSFDPAFLTNTRDPRREREDEMARAWLRARLVRKCHRAATGIMI
                     AAMAAIALPWLGVSAFAWWIASGAAVVMLALAVVMFVQGGVSSGIITLVCALAALPLW
                     VHFSPQVFLTGVELWEMLSSQWRESLERP"
     gene            complement(3347..4204)
                     /locus_tag="PLP58_11365"
     CDS             complement(3347..4204)
                     /locus_tag="PLP58_11365"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013362.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RsmB/NOP family class I SAM-dependent RNA
                     methyltransferase"
                     /protein_id="HRJ09162.1"
                     /translation="MRASLPDWLDELMARELGADWPAIRDSLNQPAAVFLRVNTLKTD
                     RRSLKVRLAQEGFITETVTDIPTALRMAQRYNVFGLAAFKEGLFEVQDASSQRIAPFL
                     QVEPGQKVVDACAGAGGKALHMAALMQNKGRVIALDIHEWKLKELRKRAARAGVDVIE
                     TRPIESAKTFKRLAGHADRLLLDVPCSGLGVLRRNPDAKWKLRPEEIARLIVQQQEIL
                     QSYSRMVKPGGKMVYATCSILPAENEKQVQAFLVAQNGRWTLEEELHIDPAKTGHDGF
                     YAARLALQA"
     gene            complement(4638..6191)
                     /locus_tag="PLP58_11370"
     CDS             complement(4638..6191)
                     /locus_tag="PLP58_11370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009958013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase S10"
                     /protein_id="HRJ09163.1"
                     /translation="MMPLRPAAVLPAFFLLATSLICQAQDEAKPAPKEPAKEEAKDKA
                     KDADKPEKKSVTEHSLTINGRQVDFTTTAGLLPLKDAEGKTTADIFYIAYTRKGVDNI
                     AARPLTFSFNGGPGSSSVWLHLGLLGPRRVKLRDDGFAVPPPYELVANEYSLLDETDL
                     VFIDPVGTGYSRAAKPDEAKNFYGVNEDARSIGEFIRLYITRHARWPSPKFLIGESYG
                     TTRAAALSGELLRAHRMNLNGIMLVSTVLNFQTIWGAEGNDLPHILYLPSYAATAWYH
                     KKLPADLQTKPLADVLAEAEAFASGEFNQALLLGSSLPADKRAAVIQKMARLTGLSAE
                     FIAASDLRVPLSRFNAELLRGRRLVTGRFDSRYTSHVRDPLSNDAERDPSADAVFSAF
                     ASTFNHYVRADLKFEDDRPYNILASVGKWNWNAENQFADVSEILADSMTANPFLKVHV
                     SNGIYDMATPYYAARHTFNHLRLHPDLLKNITQDDYTSGHMMYLNLPDLKKQKEDLAR
                     FIRASVPGK"
     gene            6286..7560
                     /locus_tag="PLP58_11375"
     CDS             6286..7560
                     /locus_tag="PLP58_11375"
                     /inference="COORDINATES: protein motif:HMM:NF013015.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ09164.1"
                     /translation="MTSGILNPPRWLFVDLNSYFASVEQQLDKKLRGKPVAVVPMKTD
                     STCAIAASREAKKFGIKTGTMIRDARRMCKGLILVEACHERYVDFHHRIVREIERHYP
                     VHVIGSIDEMGCFLDDRRANEPAALDLGRRIKRGLLEKVGECITCSVGIAPNRWLAKT
                     ASDLTKPDGLEVIRLEDLPGRLEHLALTDLCGIGRNMEPRLRQAGIHSFADLWKAPPA
                     VLHRVWGGVGGDRFWHQLHGGDLDEEVVTRRTVGHSHVLAPQFRETPEAGIVARRLLL
                     KAASRMRRLGYRAREMSVSARMENSLRGEAHLRFPPVSDSHALLAHMAGLWERVLDQT
                     GRQRVKKISVTLHGLESSGTPEQLELFPALGSPVAADRARRERLSQVMDELNQHFGRD
                     SIALGFAPDKVKVFSGTKIAFTRIPDAREFQE"
     gene            complement(7572..8798)
                     /locus_tag="PLP58_11380"
     CDS             complement(7572..8798)
                     /locus_tag="PLP58_11380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009964465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-like beta-propeller repeat protein"
                     /protein_id="HRJ09165.1"
                     /translation="MKTILLSFALALSTSLQAADWPTFRGADRSDVSQETGLLKKWPA
                     KGPKQVWLNEDAGLGYAGYSIVGGTLYTMGARDAVEYVIAIDATTGKEKWSAEAGALL
                     TNGWGDGPRSTPTVDGGKVYALSGKGVLVCLDTADGKEAWRVTMESLGGKVPGWGYCE
                     SPLIEGDLVICTPGGSQGTLAAFNKNSGVKVWQSESWTDPAQYSSIIAVNHNESRQLI
                     QLTMQSVAGVNAANGSLLWKSEFPGKTAVIPTPIFQDGQVFVAAGYGVGCKSVKIGPG
                     NSVEEVYAGTDMVNHHGGVILHEGHLYGYSDRGGWTCMDFKTGAVKWTDKSLGKGAIH
                     CADGMLYLLEEKTGVVALIKASPRGWDEQGRFTLSPQTQQRNPKGMIWTHPVVSGGKL
                     YLRDQELLFCFDVSGK"
     gene            8908..9975
                     /locus_tag="PLP58_11385"
     CDS             8908..9975
                     /locus_tag="PLP58_11385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009960909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PA0069 family radical SAM protein"
                     /protein_id="HRJ09166.1"
                     /translation="MDTPANPTQMRGRGAAVNPEQRFASLHVQYDEVEAPERVATKFF
                     KDHASSVISRHSSPDLPFEASLNPYRGCEHGCAYCYARPTHEWLGFSSGVDFESRILV
                     KEDAPRILRETLSHSKYLPEKLSLSGVTDPYQPVEKRLQITRGCLAVLAECRHPVVMI
                     TKNHLITRDADHLAELARHQAAAAYISITTLDADLARKLEPRASAPRMRLEAISVLAA
                     AGVPVGVSVAPIIPGLNDSEIPAIMQAARGAGAQFAGYTVVRLPFGVKEVFATWLERH
                     FPGMKEKVLGRIAETQGRTLSHGEFGKRLKGVGVWSEQIAQLFQVSRLRAGMAHRRPE
                     VSAAAFRRPADPGGQMELFPQ"
     gene            10004..11152
                     /locus_tag="PLP58_11390"
     CDS             10004..11152
                     /locus_tag="PLP58_11390"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HRJ09167.1"
                     /translation="MRPATLHAMKTVIAAASHAKTALRLLAASLMALTAHAREPAPLD
                     AAVAARVDGAVMAEMERQELVGAAIGVLREDGIVYLKGYGLADREKKEPVTVETVFNW
                     ASNSKPLLGVAAMQLVERGKLDLDADVRVLVPEFPDKKEVITMRQLLCHQSGIPHYGP
                     VIVIPTRRGYREPMPFLDPVNSLDKFNKTPLSHPPGTRVLYSSYACILASAVMQRAGG
                     EPCHQQIMSRIARPLGMTSLQLDMERDRQPHWAAGYMKDKSGRVVPAPESAEYWKHGA
                     GGYKSNIRDFALWARAMLRQSLLSPPVEAEMLTPQKLRDGTTTDRSLGWMVDQDGARV
                     HHGGKQNEATSRLVLYPKRDHGMVVLTNCGHGDPGAITTAVYKALNER"
     gene            complement(11156..11680)
                     /locus_tag="PLP58_11395"
     CDS             complement(11156..11680)
                     /locus_tag="PLP58_11395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ09168.1"
                     /translation="MTGLIEVKVADLAQLFNSMDPSPFHERDLDHDAEEFIVSWAQEH
                     PREHDLRLLIHLSRPPAGEMASLENAKDSIRHYFDYRAGMLWREFRRLMREGRAALLI
                     GLAFIALCQVAAGTLFHGEQTWHGIAREGLTVLCWVAMWRPIEIYLYRWWPLLRLRRL
                     YQRLSVMEIEIRVG"
     gene            complement(11684..12316)
                     /locus_tag="PLP58_11400"
     CDS             complement(11684..12316)
                     /locus_tag="PLP58_11400"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009964305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HRJ09169.1"
                     /translation="MDQAPLPYAPAALEPHIDAMTMEIHFGRHHLAYINNLKAALEAA
                     SLQSNDIAALVGDLSKVPEAQRMAVRNNGGGHVNHSWFWQWMAPAGSGASGPEGKLGA
                     AIQSTLGSVDDFKKLFAEAGAKRFGSGWAWLIVRKDGSLAVTSTPNQDNPLMKGVVPE
                     GEEGTPILGLDVWEHAYYLKYQNKRPDYITAWWNVVNWAAVAKGYEAAKA"
     gene            12560..14002
                     /gene="gnd"
                     /locus_tag="PLP58_11405"
     CDS             12560..14002
                     /gene="gnd"
                     /locus_tag="PLP58_11405"
                     /EC_number="1.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009964470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004616 - phosphogluconate dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="decarboxylating NADP(+)-dependent
                     phosphogluconate dehydrogenase"
                     /protein_id="HRJ09170.1"
                     /translation="MSKKSDFGLIGLAVMGQNLVLNVESRGFQVSVYNRTTSVTDEFV
                     AKHPGKALVGCHTLEDFVQSLALPRKIHIMVKSTAVKDSDRDAVDAVIEQLIPLLDQG
                     DIVIDGGNSFYQTTERRDAYLSEKGLRFIGAGVSGGEEGARKGPSIMPGGPASTWEVM
                     KPIFESIAAKVDGEPCVIHIGPGGAGHYVKMIHNGIEYGDMQLICEAYNIFKHAGFTT
                     DEMAAIFNEWNEGDLLSYLIQISGKALEQKDPETGKPVVELIVDKAGQKGTGQWTLLN
                     AAENAVVISTINAAVEARILSSQKKQRVAASKELTGPAVNITTPKAELVKKVHDALYA
                     SKIISYAQGLDLIKTMGEKKNWGLDLGRIAAIWRGGCIIRARFLNRITDAYRTNPQLA
                     NLMLDPFFKDVLSKTQQSWREVVSIATLNGIPVPAFSASLGYYDSYRAERLPANLLQA
                     QRDFFGAHTYERLDKPEGQFFHTEWPEVIG"
     gene            complement(14374..15231)
                     /locus_tag="PLP58_11410"
     CDS             complement(14374..15231)
                     /locus_tag="PLP58_11410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ09171.1"
                     /translation="MSKPTTDQLARKSIAVLVAHVRDHQNEPLPNLSYEEVAERIGRL
                     TYKGIPFARGMGSQVFKYMGHLFEGQKLHGVSSIPHLQCLVGSKSDPLRLPEDGYEEF
                     VPGYSKLSRPERIAHIEAEKSKIIAFGDAWNLVLKRFGQETVDGLSKVSPAFTSSGES
                     PRHRALKEHVIKNPGIVGASKYAEVHPEYILPSQDRLDILFKQPKRWTAVEVKSRVSD
                     SVEGDYKRGLYQIVKYRALIDAMRRDPGHNVPVEVTVLLVLESSLPEDLQPLAEKLDV
                     EAILTRLPE"
     gene            15324..15500
                     /locus_tag="PLP58_11415"
     CDS             15324..15500
                     /locus_tag="PLP58_11415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ09172.1"
                     /translation="MNPKNSPQPVTKAIRLKPSVTDYATFVATTSKVLGTTGPASRKT
                     KPNKLVLSVANLGS"
     gene            15746..15916
                     /locus_tag="PLP58_11420"
     CDS             15746..15916
                     /locus_tag="PLP58_11420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ09173.1"
                     /translation="MDPTGQHSLFFNDAFQTLAIAALPRNCYLKDGDLLPFDVILCQP
                     DEELEAFLRLYD"
     gene            16648..17289
                     /locus_tag="PLP58_11425"
     CDS             16648..17289
                     /locus_tag="PLP58_11425"
                     /inference="COORDINATES: protein motif:HMM:NF018178.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1080 domain-containing protein"
                     /protein_id="HRJ09174.1"
                     /translation="MKHFLPLLLLALGAHAGESVSLFDGKTFAGWTGVDGKAPGPGWS
                     IVDGELRLDGAGGNLISEKEYSNFDLEWEWKLAEGGNNGVKYWVTKVGGKEWLGIEYQ
                     MLDDNKHPDAKNGAKRLTASFYDIQPPSVPTTVSPPGEWNHSRIVAKDGRLEHWLNGV
                     KVGEADITSEDWKQKLGESKFKTKEGFAPGKGRIMLTDHKDKTWFRNIRIKEL"
BASE COUNT         3170 a         5257 c         5619 g         3366 t
ORIGIN      
        1 ggcagggggg ggtggcgggg cggcaggggg agtggacgtg agtttggcga tttcagcctc
       61 gctgagtccg acattgacga cgagtccggg gagttggcct tcgagcgcct tggctccggc
      121 tttggtgacg cctgtctgcc agacaaagag ttttttcaac cccttggcac cagcgagttt
      181 ggaaatgcca gcatccgtgg ttttggtgcc gtagagattg aggtattcga gggtctgcaa
      241 ggcggcgagt ttggccaggc cggcatcggt gactttggtg ttttccaaat gcaggcgctc
      301 aagggcggtc attttggcga gggtatccgc ggcggcgtcg gtgacctggc tgcgggcgag
      361 gtccacccag acgaggtgtt tggcgatggg ttcgagcagg gcaagttcct tgtcgccgaa
      421 tttgtcggtg gcattgatga cgctcagacg cagttgctcg gtgtcctggg caagcggcat
      481 gagtgtggcg ttgagcgcct ggattttggc ggtgatttcg gtgatctgtt ttttctccgc
      541 atctgtgagc ggagcgggct tgctcttggc gggcgcggcg gtggttttgg cgtcctgttt
      601 gggcaggccc ttggcgagca ggagtgcgag ggctgcctcg gcgtcgggag tgggcttggc
      661 ggctgcgaca gtggcagtct cggaggcgcc ggtttcgatc caccacttca agacggccag
      721 ttcctccttg gagatctgcg gctcatcctc cggcggcatg tgctcgtcgt cgtccgctgg
      781 caggttgatg cggatgagca tgaggctttc ggcgggtttg ccggcgacga cattgttttc
      841 cccttcgctg ccgcctttga gaatggaggc gtagtcgtgg agttgcagct tgcctttctt
      901 tttttcctca ccgtggcaac ccacgcattt ggctgccatg atgggggcga tgacatcgcg
      961 atagacgacc gccttggcgg ccagggcggg atcgagcttg gccgcagcgg caggttttgg
     1021 ggcgtcggct ttcttttctt caggcttggc gggggcgggt gccgcaggct ttggagcgtc
     1081 cgccgcaggt ttcgcgtcag cggcgggagt ggcggcgatg aagggggcgg gtttggcgtc
     1141 gtcttcggcg gtccagcctg tgttgatgtg cgcgccgggc agcctggctt tgagctgggc
     1201 cactccggct ttggtgacgc cgctttgcca tagatagagg gccttcaatt tggcaagacc
     1261 ggcgagtttt tgcaggccgg cgtcggtgac tttggtgttg taaaggttga ggtattcgag
     1321 gctggccaga tccttgagat gatcgagtcc ggcatcaccg atcccggtgt tttcaaggtg
     1381 cagttcggcg aggtttttca tcgccgccac agctttgagg ccggcatcgg tgattttggt
     1441 gcgggcgagg tcgagcgagg cgatcttgtc cgcgacgggt atgagaggtt cgaggccggc
     1501 gtcggtgaag tctttggcga cattgagagc ggtgaagcgc caggctttgc catcagcggc
     1561 cacgggcatg agcagggcgc cggaggcgtt gaccttgtcg gcggccgctt tcatggccgg
     1621 gtcggcggat gcgggctggg cttggaggga accggccagc agggtgaagg cgaagagggt
     1681 gtggatcggg cgcataaggg ggattgggga aaaggattcg tcaggacgcg gtctgacctt
     1741 tcatgacagc ttcggcggcg gttttgaggt tttcggggag tttggcctcg tccatcttca
     1801 gggtttcgga cgcgccctgc tcgatccacc agcggaacag cgccaattcg tcggcggtca
     1861 tctgctcctt gcctttgggg ggcatgtgct cgtcgtcatc ctcagggagc aggatgcgtt
     1921 cgatggccag gctctcgtcc ggcttgcctg gaatgacgct ttctccactg tcaccgccct
     1981 gcatgaggag ggcgtaggtg tccatgcgca ggccgccttt ggttttgtcc tcgttgtggc
     2041 aggtgttgca cttggcttcg aaaatgggca tcagcacatg ctggaagacg agcttgtcgg
     2101 agctcgtcgc tcccactggc ggtgtttcgg caggcggaag tgtgtcaggt gtttccagct
     2161 ccgccttggg tttctcgaca aagccgagca tccaattttc catggcaatc atccggcttt
     2221 tcagcggctc aggcgcgtgc gcggtgaggt atttgctgcc gtggctcata ttgccgccga
     2281 agtgcgcgcc aagtcccatg aggccgaagg aggcgaaaaa cagcgcgcgg tagaagtgca
     2341 gcagcccgcg ctcctcctgc cccatgccca gcatccgcac cacgagcgcc agcaggatgc
     2401 ccgatgtgcc gatgatcgct aatgtttggt gcaggctgaa atttccccct gcatacccgc
     2461 cttcgcgtga aagcaaaata cccgccagca ccgccatcac cgcgcctgcg gagccgacca
     2521 gaagcgtcag cagtgccgcg ccgcccagct tttcctcgcc ggagcgtgtg aggcagccga
     2581 gctccatcag acaaagcagg ccgagcgcgc ccacaggcag atgcaggaaa atcggatgga
     2641 aacgtccgat gaagagcagc agggaggacg tttcaccctc caccggctcg aagcgcggag
     2701 ggaaggcgat gagcacggtg acgtaaccca ggatgatgag cagagcgagc gcccaggtgc
     2761 ccatgcgggg cttggaggag gagtcaaagg aggacatggt tgaaatgccg ggggttggcg
     2821 tgattacgga ggctggcaag cgaatcgttc actccatact tgcggaatca tgcccggatc
     2881 atggcctttc caggctttcc cgccactgac tggagagcat ttcccaaagc tcaactcctg
     2941 tgaggaacac ttgtggagaa aaatgcaccc atagcggcag cgcagccagc gcgcacacca
     3001 aggtgatgat gccgctggag acgccgcctt ggacaaacat caccactgcc agcgccagca
     3061 tcaccaccgc ggcaccgctg gcgatccacc aggcaaaggc gctgacgccc agccagggaa
     3121 gcgcgatggc ggccatggcg gcgatcatga tgcctgtggc cgcgcgatgg cacttcctga
     3181 ccagccgggc gcgcagccat gcgcgcgcca tctcgtcctc gcgctcgcgg cgcgggtcgc
     3241 gggtgttggt cagaaaggcg gggtcgaagc tcatgcgtct gtcatcacgc tcgcggggcg
     3301 ccggttgtca aatccacgcg agttttcatt cgacgaaggc gggccgtcag gcttggagtg
     3361 ccagacgcgc ggcataaaag ccgtcgtgcc cagttttggc ggggtcgatg tggagctcct
     3421 cctccagggt ccagcggcca ttttgcgcga cgaggaatgc ctggacctgc ttttcgtttt
     3481 ccgcgggcag gatggagcag gtggcataca ccatttttcc gccgggcttc accatgcggc
     3541 tgtagctctg caaaatctcc tgctgctgca cgataaggcg cgcgatctcc tccggcctga
     3601 gcttccactt ggcgtctgga ttccgccgca gcacgccgag gccggagcag ggcacatcga
     3661 gcaggaggcg gtccgcatgg ccggcgaggc gcttgaaggt cttggcgctt tcgatggggc
     3721 gcgtttcgat gacatccact ccggcgcggg cggcgcgttt gcgcagttcc ttgagcttcc
     3781 attcgtggat gtcgagggcg atgacgcggc ccttgttttg catgagggcg gccatgtgca
     3841 gcgccttgcc gccagccccg gcgcaggcgt caacgacctt ctgtcctggc tcgacttgaa
     3901 ggaagggcgc gatcctttgg gatgaggcgt cctggacctc gaagaggcct tctttgaatg
     3961 cggcgaggcc gaagacgtta taacgttggg ccatgcgcag ggcggtgggg atgtccgtca
     4021 cggtttcggt gatgaagcct tcctgcgcca ggcgcacttt aaggctgcgg cggtcggtct
     4081 tgagggtgtt gacgcgcaga aagacggcgg cgggctggtt caggctgtcg cggatggccg
     4141 gccagtccgc gcccagctcg cgcgccatga gttcgtcgag ccagtcgggc aatgacgcgc
     4201 gcacggccgg cagcgcgtct ttttgccgta ggcgggcctg gatggcgctg gcgttgatgc
     4261 catagagttc gtcgaaggcg ggcagttcgt gtccggcttg gatccaatag gcggcccaga
     4321 cctgccacag gcgctcctca gtgacgcggt cgccctggtc gtgctcggca tccggcaggc
     4381 ccgccgcgtg ccagagccag cgccaccagc ggacgatgtc atagacgccc tcggcgaaga
     4441 tgcggcggtc acggctgccc catttgcgcc ggtttttgag gacgtgctcg acgcatttgt
     4501 ccgcataacg cctctcgacg aaaatttcgc aaagggctga gacagtttcg gcgatgatga
     4561 gatgggggag tttcaggggc ggggaagggg tgggattttt ctggggcggg catcacgggg
     4621 gcctgccatt cggtgtgtca ctttccgggc acggaggcgc ggatgaagcg tgccaggtcc
     4681 tctttctgct ttttgaggtc gggcaggttg aggtacatca tgtggccgga ggtgtagtcg
     4741 tcctgggtga tgtttttgag caggtcggga tgcaggcgca ggtgattgaa ggtgtggcgc
     4801 gcggcatagt agggggtggc catgtcatag atgccattgg agacgtggac tttgagaaaa
     4861 gggttggcgg tcatgctgtc ggcgaggatt tcggagacat cggcgaactg gttttcggcg
     4921 ttccagttcc atttcccgac gctcgccagg atgttgtagg ggcggtcgtc ctcgaacttc
     4981 aaatccgcgc gcacataatg gttgaaggtc gaggcgaagg cgctgaagac cgcgtcggcg
     5041 ctggggtcgc gctcggcatc gttgctgagc ggatcgcgca catgactggt gtagcggctg
     5101 tcaaagcggc cggtgacgag gcgccggccg cgcagcagct cggcgttgaa gcggctgagc
     5161 ggcacgcgaa ggtcggaggc cgcgatgaac tccgcgctca ggccggtgag gcgggccatt
     5221 ttctggatga ctgcggcgcg tttgtcagcc ggcagggagg agccgaggag gagcgcttga
     5281 ttaaactcgc cgctggcgaa ggcctccgct tcggcaagca catccgccaa aggtttcgtt
     5341 tggagatcgg cgggcagttt tttgtgatac caggcggtgg cggcgtagct gggcaggtag
     5401 aggatgtgcg gaaggtcgtt gccctcggca ccccagatgg tctggaagtt gagcacggtg
     5461 gagacgagca tgatgccgtt gaggttcatg cggtgcgcgc gcagcagctc gccgctgagc
     5521 gcggcggcgc gggtggtgcc gtagctctcg ccgatgagga acttgggcga tggccagcgc
     5581 gcgtggcggg tgatgtaaag ccggatgaac tcgccgatgc tgcgcgcgtc ctcgttgacg
     5641 ccgtagaaat tttttgcctc atcgggcttg gcggcgcggc tgtagccggt gcccacgggg
     5701 tcaatgaaga cgaggtcggt ttcatcaagc agggagtatt cgttcgccac cagctcatag
     5761 ggcggcggca cggcgaaacc gtcatcccgc agtttcacgc ggcgcggtcc cagcaggccc
     5821 aggtgcagcc acacagagga ggatcccgga ccgccgttga aggaaaaagt cagtggcctg
     5881 gcggcgatgt tgtccacgcc cttgcgggtg taggcgatgt aaaaaatgtc cgccgtggtc
     5941 ttgccctcgg cgtccttgag cggcagcagt ccggcggtgg tcgtgaagtc cacctgccgg
     6001 ccgttaatgg tgaggctgtg ctcggtgacg cttttcttct ccggcttgtc ggcgtccttc
     6061 gccttgtctt tggcctcttc cttggcgggt tccttgggcg cgggctttgc ctcgtcctgc
     6121 gcctggcaaa tgaggctggt ggcgagcaga aagaaggcgg gcaggactgc ggctggacgt
     6181 aagggcatca tgacgcgcac gattggcggg gatcgggtgt gggtgcaaac attcttccga
     6241 taactgtttt cttgaacagt ttaaaactga gtttagcctt ggcttgtgac ctccggcatt
     6301 ttaaatcccc cgcgctggct gttcgtggac ttgaacagct acttcgccag cgtcgagcag
     6361 cagctcgaca aaaaactgcg cggcaagccg gtggcagtgg tgccgatgaa gacagactcc
     6421 acctgcgcca tcgcggcgag ccgcgaggcg aagaaattcg gcatcaaaac cggcacgatg
     6481 atccgcgacg ccaggcgcat gtgcaaaggg ctgatcctgg tggaggcgtg ccatgagcgg
     6541 tatgtggact ttcatcaccg catcgttcgc gagatcgagc ggcattaccc ggttcatgtg
     6601 atcggctcga ttgatgagat gggctgtttt ctggacgacc ggcgcgccaa tgagccggcg
     6661 gccctcgatc ttgggcggcg catcaaacgc gggctgctgg aaaaagtcgg cgagtgcatc
     6721 acctgctcgg tgggcatcgc tccaaaccgc tggctggcca agacggcgag cgaccttacc
     6781 aagccagatg gtctggaggt catccgtctg gaggatttgc caggcaggct ggaacacctg
     6841 gcattgacgg acttgtgcgg catcgggcgg aacatggagc cgcgtctgcg ccaggcgggc
     6901 attcattcat tcgcggattt gtggaaggcg ccgcctgcgg tgctgcaccg tgtttggggc
     6961 ggcgtgggcg gggaccgctt ttggcaccaa ctccacggcg gagacctgga cgaggaggtg
     7021 gtgacgcgtc gcacggtggg ccacagccat gtgctggcgc cgcagttccg tgaaacgccg
     7081 gaggccggca ttgtggcgcg taggctgctg ctaaaggcgg ccagccgcat gcgccgcctg
     7141 ggctaccgcg cgcgtgagat gagcgtcagc gcgcgcatgg agaacagctt gcgcggggag
     7201 gcgcatctgc gcttcccgcc cgtgtcagac agccacgcgc tgctggccca catggccggg
     7261 ctgtgggagc gcgttctgga ccagaccggg cggcagcggg tgaaaaaaat ctccgtcacc
     7321 ctgcacgggc tggaatcctc cggcacgccc gaacagcttg agcttttccc cgcgctcggc
     7381 agtccggtgg cggcggaccg cgcgcggcgg gagcggctct cgcaggtgat ggacgagttg
     7441 aaccagcatt tcgggcggga cagcatcgcg ctcggcttcg cgccggacaa agtcaaagtc
     7501 ttctccggca cgaagatcgc cttcacacgc atcccggatg cgagggagtt ccaggaatga
     7561 cgcgcggcgc ctcacttccc gctcacgtcg aagcagaaga gcagctcctg gtcgcgcagg
     7621 tagagttttc cgccgctgac cacggggtgg gtccaaatca tgcccttggg gttccgctgc
     7681 tgggtctgcg ggctgagggt gaagcggccc tgctcgtccc agccgcgcgg gctggccttg
     7741 atgagcgcca ccactccggt tttttcctcc agcaggtaaa gcatgccgtc agcacagtgg
     7801 atggctcctt tgccaaggct cttgtcggtc catttcaccg caccggtttt gaagtccatg
     7861 caggtccagc cgccacggtc ggaatagccg taaagatggc cttcatgcag aatgacgccg
     7921 ccgtggtggt tgaccatgtc agtgccggca tagacttcct ccaccgaatt gccagggccg
     7981 attttcaccg acttgcatcc gactccgtaa ccagcggcca caaagacctg cccgtcttga
     8041 aaaatgggcg tggggatgac ggcggtcttg ccggggaatt ccgacttcca gagcagcgag
     8101 ccattggcgg cattgacgcc ggcgacgctc tgcatggtga gctggatgag ctggcgggac
     8161 tcgttgtggt tgacggcgat gatggaggag tattgcgcgg ggtcggtcca ggactcgctt
     8221 tgccagactt tgacgccgct gtttttattg aaggcggcca gggtgccctg ggagccgccg
     8281 ggggtgcaaa tgacgagatc accctcaatg agcggactct cgcagtaacc ccagcccggc
     8341 accttgccgc cgaggctctc catggtcacg cgccaggcct ccttgccatc ggcggtgtcg
     8401 aggcagacga gcacgccttt gccgctcagg gcatagacct tgccgccgtc cacggtgggt
     8461 gtgctgcgcg ggccgtcgcc ccagccgttg gtgagcagcg ctccggcttc ggcggaccat
     8521 ttttctttgc cggtggtggc gtcaatggcg atgacgtatt cgacggcgtc gcgcgcgccc
     8581 atggtgtaaa gcgtgccgcc gacgatggag tagcccgcgt agcccaggcc ggcgtcctcg
     8641 ttgagccaga cctgcttcgg ccccttggcg ggccattttt tcagaagtcc ggtttcctgg
     8701 gaaacgtcgg agcggtcggc tccgcggaaa gtgggccagt cggcggcctg aaggcttgtg
     8761 gaaagcgcga gcgcaaagga gaggaggatc gttttcatgg tgtcggctga tgtgaacgcc
     8821 agtggaagcc tgactttgcc cggatgtaaa ggtttctctg aatcttcgca gcccttgcca
     8881 gacaggccgc gcccgccagg atgcggcatg gacacaccgg ccaatcccac ccagatgcgc
     8941 ggacgcggcg cggcggtgaa cccggagcag cgtttcgcca gcctgcatgt gcagtatgac
     9001 gaggtcgagg cgccggagcg cgtggcgacg aagtttttca aggatcacgc cagcagcgtc
     9061 atcagccggc acagcagccc ggacctgccc tttgaggcga gcctgaaccc ctaccggggc
     9121 tgcgagcatg gttgcgctta ttgttatgcg cggcccactc atgagtggct ggggttctcg
     9181 tcgggggtgg atttcgaaag ccgcatcctg gtgaaggaag acgcgccccg catcttgcgc
     9241 gagacacttt ctcattcgaa gtatttgccg gagaagctct ctctgagcgg agttactgat
     9301 ccctatcaac cggtggagaa gcggctgcaa atcactcgcg gctgcctggc cgtgctggcc
     9361 gaatgccggc atccggtggt gatgatcacg aaaaaccacc tcatcacgcg ggacgcggac
     9421 catctggccg aactcgctcg ccatcaggca gcggcggcct acatctccat caccacgctc
     9481 gacgcggacc tggcgcgcaa gctggagccg cgcgcctccg ctccgcgcat gaggctggag
     9541 gccatctccg tgctggcggc ggcgggtgtg ccggtgggcg tgtcggtggc accgatcatc
     9601 cccggcctca atgactcgga gatacccgcc atcatgcagg cggcgcgggg ggcgggcgcg
     9661 cagttcgctg gctacacggt ggtgcggctg ccctttggcg tgaaggaggt cttcgccacc
     9721 tggctggagc ggcattttcc cggcatgaag gaaaaggtgc tcggacgcat cgccgagacg
     9781 caggggcgca ccctctcgca tggcgagttt ggcaagcggc tcaaaggtgt gggtgtgtgg
     9841 tcggagcaga tcgcgcagct ttttcaggtg tcccgcctgc gcgcgggcat ggcgcaccgc
     9901 aggccggagg tcagcgccgc cgcttttcgc aggccggcgg atcccggcgg gcagatggag
     9961 cttttcccgc aatgacctgc cgcattccgt cttgaacccg ctcatgcgtc ccgccacact
    10021 tcatgccatg aagaccgtca tcgctgccgc ctcccatgcc aaaaccgctc tccgcctgct
    10081 ggccgcgagt ctgatggcgc tcaccgcgca tgcccgggag cccgcgcctt tggatgcggc
    10141 ggtggccgcg cgtgtggacg gggcggtgat ggccgagatg gagcgccagg agctggtggg
    10201 cgcggccatc ggtgtgctgc gggaggacgg catcgtttat ctcaaaggct acggactggc
    10261 cgaccgcgaa aaaaaagaac cggtcaccgt ggagaccgtc ttcaactggg catcgaactc
    10321 caaacccctc ctcggcgtgg ccgccatgca gctcgtggag cgcggcaagc tcgacctcga
    10381 cgccgatgtg cgcgtgctgg tgcccgagtt tcccgacaaa aaggaggtca tcaccatgcg
    10441 ccaactgctc tgccatcaga gcggcattcc gcattacggc cccgtcatcg tcatccccac
    10501 gcggcgcggc tatcgcgagc cgatgccctt tctcgatccg gtcaactcgc tcgacaagtt
    10561 caacaagacg ccgctttccc acccgccggg cacgcgggtt ctttattcat cctatgcctg
    10621 catcctcgcc agcgcggtga tgcagcgcgc cggcggggaa ccctgtcatc agcagatcat
    10681 gagccgcatc gcccgccctc tcggcatgac cagcctgcag ctcgacatgg agcgcgaccg
    10741 ccagccgcac tgggccgccg gttacatgaa ggacaaatcc ggccgcgtcg tgcccgcgcc
    10801 ggagagcgcc gaatactgga agcacggcgc tggcggctac aaatccaaca tccgcgactt
    10861 cgccctctgg gcgcgcgcca tgctgcgtca aagcctgctc agcccgccgg ttgaggcgga
    10921 aatgctcaca ccgcaaaagc tccgtgacgg caccactacc gaccgcagcc tcggctggat
    10981 ggtggaccag gacggcgcgc gcgtgcatca cggcggcaag cagaacgagg ccacctcccg
    11041 cctcgttctc tacccgaagc gcgatcatgg catggtcgtg ctgaccaact gcggccacgg
    11101 tgatcccggc gccatcacca cggcggttta caaggcgctc aacgaacggt gacggtcatc
    11161 ccacgcggat ttcgatctcc atgaccgaca gtctttgata gagacggcgc agcctcagca
    11221 gcggccacca gcggtagagg taaatctcga tgggccgcca catggccacc cagcacagca
    11281 cggtcagccc ctcgcgcgcg atgccgtgcc aggtctgctc cccgtgaaac aatgtccccg
    11341 ccgccacctg gcagagcgcg atgaatgcca gcccgatgag cagtgccgcg cgcccctccc
    11401 gcatgagacg gcggaactcc cgccagagca tccccgcgcg gtagtcaaag taatggcgga
    11461 tggagtcctt ggcgttttcc agggaggcca tctccccggc gggcggccgg gagaggtgga
    11521 tgagcaggcg caggtcgtgc tcgcgcggat gctcctgcgc ccagctcacg atgaactcct
    11581 ccgcgtcatg gtcgaggtcg cgctcgtgga agggcgaggg gtccatggag ttgaacaact
    11641 gcgccaggtc cgcgaccttg acctcgatca gaccggtcat gggtcaggct ttggcggctt
    11701 cgtaaccctt tgccacggcg gcccagttca ccacattcca ccacgcggtg atgtagtcgg
    11761 ggcgcttgtt ctgatacttc agataatagg cgtgctccca gacatcgagt cccaggatgg
    11821 gcgtgccttc ctctccctcc ggcaccacgc ccttcatcag cggattgtcc tggttcggcg
    11881 tcgaggtcac ggccagcgag ccgtccttcc tcacgatcag ccaggcccag cccgagccaa
    11941 aacgcttggc ccccgcctcg gcgaagagtt ttttgaaatc gtccacgctg cctaaggtgc
    12001 tctggatggc cgcgcccagc ttgccctccg gcccggacgc gccgctgccg gcgggagcca
    12061 tccactgcca gaaccacgag tggttcacat ggccgccgcc gttgttgcgc acggccatgc
    12121 gctgcgcctc gggcaccttc gagaggtcgc cgaccagcgc ggcgatgtcg ttggactgaa
    12181 gacttgccgc ctccagcgcc gccttcagat tgttgatgta ggccagatgg tgcctgccga
    12241 agtgaatctc catcgtcatc gcgtcaatgt gcggctccag cgcggcgggc gcgtagggca
    12301 gcggcgcctg gtccatgccg atgacggcgg cgtggctggt ggatgtgatg gccgagccgg
    12361 ccagtgcggc tcccgtggtg gtgatgaatg tgcggcggtt catggtgtgg gctgatgaat
    12421 acgccgccgg cgcgcaaatg tcactgcgga aagtgctcct cccctggcgg gcagggagcc
    12481 tttgattcat gcggatcatg aacaaaatcc cctttgccaa agcggggcgg gggggtatgt
    12541 ctgccgcccc aaaaatacta tgagcaaaaa aagtgacttc ggtctgatcg gcctcgccgt
    12601 catgggccaa aacctcgttc tcaacgtcga aagccgcggc ttccaggtca gcgtctataa
    12661 ccgcaccacc agcgtcaccg acgaattcgt cgccaagcac cccggcaaag ccctcgttgg
    12721 ctgccacacc ctggaggact ttgtgcaaag cctcgccctc ccgcgcaaaa tccacatcat
    12781 ggtgaaatcc accgccgtga aggacagcga ccgcgacgcc gtggacgcag tgatcgagca
    12841 gctcatcccc cttctcgacc agggcgacat cgtcattgac ggcggcaaca gcttctacca
    12901 gaccaccgag cgtcgcgacg cctacctcag cgaaaaaggc ctccgcttca tcggcgccgg
    12961 cgtctccggc ggtgaggaag gcgcccgcaa aggcccctcc atcatgcccg gcggccctgc
    13021 cagcacctgg gaagtgatga agcccatctt cgaatccatc gccgcgaagg tggacggcga
    13081 gccctgcgtc atccacatcg gccccggcgg agccggtcac tatgtgaaga tgatccacaa
    13141 cggcatcgaa tacggcgaca tgcagctcat ctgcgaagcc tacaacatct tcaagcacgc
    13201 cggcttcacc accgacgaga tggccgccat tttcaacgag tggaacgagg gcgacctcct
    13261 cagctacctc atccagatct ccggcaaggc tttggagcaa aaagaccccg aaaccggcaa
    13321 acccgtcgtc gagctgatcg tggacaaggc cggccagaaa ggcaccggcc agtggacgct
    13381 gctcaacgcc gccgaaaacg ccgtcgtcat cagcaccatc aacgccgctg tcgaggcccg
    13441 catcctttcc agccagaaga agcagcgcgt tgccgccagc aaggaactca ccggccccgc
    13501 cgtcaacatc accacgccga aggccgagct ggtgaagaag gtccacgacg ccctctacgc
    13561 ctccaagatc atcagctacg cccagggtct cgacctcatc aagaccatgg gcgagaagaa
    13621 gaactggggc ctcgacctcg gccgcatcgc cgccatctgg cgcggcggct gcatcatccg
    13681 cgcccgcttc ctcaaccgca tcaccgacgc ctaccgcacc aacccgcagc tcgccaacct
    13741 catgctcgac ccgttcttca aggacgtgct cagcaagacc cagcagagct ggcgcgaagt
    13801 ggtgagcatc gccaccctca acggcatccc cgtccccgcc ttcagcgcct ccctcggcta
    13861 ctacgacagc taccgcgccg aacgcctccc cgccaacctt ctgcaggctc agcgcgactt
    13921 cttcggcgcc cacacctacg agcgcctcga caaacccgaa ggccagttct tccacacgga
    13981 gtggccggag gtgatcggct gatccaaaag gaccgcggac actcctgtcc gctgcatcaa
    14041 ctcaaaacaa aggcggctga ggcgactcag ccgccttctt gcatcttgga ttcagtccgc
    14101 tatcctctgg cccatgcaaa ccatcgccca gaaacccgcg cgtgaaggct ggcaggccgt
    14161 gaactgctcc agcctggacg acatgcgcac gcaaaacatc cgcttttggc aaaacgcggg
    14221 cggtgccgcc atccgcaagg ctgcctggga actggtcgtc gaaacctggc aggcacagca
    14281 ccgcgatccc gatgaactcc gatttcaaag acttgcttcg gttgccactt cgagtcagcg
    14341 tcctgggcat ttaaggccta ccagatttag cgttcactcg ggcaaacgtg tgagaatagc
    14401 ctcgacatcc agcttttccg ccaaaggctg gagatcttct ggcaaagagc tttcgaggac
    14461 tagcaacacc gtcacttcta ctggcacgtt atgccccggg tcacgacgca tcgcgtcaat
    14521 caaggcacga tacttcacaa tctggtaaag acctcgcttg tagtcgcctt cgacgctgtc
    14581 cgaaacacga gacttaactt ccactgcagt ccatcgtttc ggttgtttga aaaggatgtc
    14641 cagtcggtcc tgcgatggca ggatgtattc gggatgcacc tcagcatatt tcgaagctcc
    14701 cacgattcca ggattcttta tcacatgctc cttgagagca cggtggcgag gggattcgcc
    14761 tgaactggtg aaggctggcg ataccttcga cagtccatcc acagtctcct gtccgaatcg
    14821 cttcagcaca aggttccatg cgtcaccgaa ggcaatgatc ttggacttct cagcctcgat
    14881 gtgagcgata cgctcgggtc gggacagctt gctatagcct ggaacgaact cctcgtagcc
    14941 atcctcgggc agcctcaatg gatctgattt gcttcccacg aggcattgta ggtgaggtat
    15001 gctggaaacc ccgtggagct tctgcccttc gaacaaatgg cccatgtatt tgaagacctg
    15061 gcttcccatg cctctggcga acggtatgcc tttgtatgtc agtcgaccaa ttcgttcagc
    15121 gacttcttca tagctcaggt tgggcagggg ttcattctgg tgatcgcgaa catgagcaac
    15181 aagcacggcg atgctttttc tcgccagctg gtcggtagtg ggttttgaca tgaagtagtg
    15241 gacgttagga gctgatattg atctgcgtca agcccccaaa ggaacgacgt taacgtcttc
    15301 tgagcagtac ggataccctc gtcgtgaatc ccaaaaacag tccgcagccc gtcacgaaag
    15361 ccattcgctt gaagccttcc gtgaccgact acgcgacctt tgtggccacc acttcgaagg
    15421 tgctgggcac gaccggaccc gcctcgcgaa agaccaagcc gaacaagctt gtgctctcgg
    15481 tcgcgaactt gggctcttga gcgttgagga agagtctgac tacatggact gcggcagcct
    15541 gctgccgctt ttccgaggca gccctgctgc cgtcggagtc tcctgactct cgaagcagcc
    15601 tctcggttgg cccgaaggcg agactccagg acgaaccatc gttctcgaaa actcgacggc
    15661 cagcaggctg gcctgccgaa agcggcagca ggctgccgca gtccaaggag agcagcattt
    15721 catcgcctcc ggctggacgc ggatcatgga tcccacgggc cagcacagct tgttcttcaa
    15781 tgatgccttt caaacgctcg ccatcgccgc cctgccgcga aactgctacc tgaaggacgg
    15841 agacctgctg ccctttgacg tgatcctctg ccagccggat gaggagttgg aggcgtttct
    15901 gcggctgtat gattgaggcg gctgccaacc gtctgccctg cccccagccg accgtcctgc
    15961 gctgccaaag ccgcctcccg gttctagagc caatagtttt ctggagggaa aggaaggtcc
    16021 ggagggcttg gaaaactctt ttctggagct gggggagact cccttggccc cagaaaactc
    16081 ttttttctag aatcaccacc tgaccttggc tacggacgac tcttttccgc agcccggaca
    16141 ccttccccac ccctggaaaa aactcttttc cgagggtcgg ggagatggac ctggtgctgg
    16201 aaaactcttt tccagcctca ggggagactc tcctgggtgc ggaaaactct tttccagcct
    16261 gagccttctt cgttttcaca gacggcaaaa gttgggtaaa caacccaaag ccgaacatga
    16321 acaagacaac gacaccaaga cgctcgtacg acgagcagtt caagcgcgac gccgtcgcgc
    16381 tgcttctaat acctcattgt gttgaacttc ctcgcctcag tgacccgatg aaaaacttca
    16441 cctgctttac tgcttgctgg ctgcttctga ggtggaccac ggagccgcac catcatgttc
    16501 agggatgaac tgacgcgcgg ttccgcaccg gttcgggtgc gtgacggcaa cagatgatga
    16561 catgcggcag aactctggtc cggggggctg gccttcaccg ccttcctgct tgacctccac
    16621 gagtttcaca gcctacgctc atgggttatg aaacactttc tgcccctgct tcttctcgct
    16681 ctcggcgcgc atgccggaga atccgtttcc ttgtttgacg gcaaaacctt cgcgggttgg
    16741 accggggtgg atggcaaggc gcccggccca ggctggagca tcgtggacgg cgaactcagg
    16801 ctcgacggcg cgggtgggaa tttgatcagc gaaaaggaat actccaactt cgacctcgaa
    16861 tgggagtgga agctggccga ggggggcaac aatggcgtca agtactgggt caccaaagtc
    16921 ggcggcaagg agtggctcgg catcgaatac cagatgctcg acgacaacaa gcatcccgac
    16981 gccaagaacg gcgccaaacg tctcaccgcc tccttttatg acatccagcc gcccagcgtg
    17041 ccgaccacgg tgagcccgcc cggcgagtgg aaccacagcc gcatcgtggc aaaggacggc
    17101 aggctggagc actggctcaa cggcgtgaaa gtcggcgagg cggacatcac ctccgaagac
    17161 tggaagcaga agctcggcga gagcaaattc aagaccaagg agggcttcgc gccggggaag
    17221 gggaggatca tgctcaccga ccacaaggac aagacctggt tccgcaacat ccgcatcaag
    17281 gagctttgaa aaatcgagaa tcgaaaatcg aaatacgagc ttcgatcacc ggcttcgaat
    17341 ttcgaatttc gagcttcgat tttcagactt caaacttccc ccttgcctcc cccagtcttc
    17401 tcgaagaact ct
//
LOCUS       DAOMYJ010000096        17346 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Prosthecobacter sp. isolate AMR_MDS_5629
            NODE_1132_length_17346_cov_19.285351, whole genome shotgun
            sequence.
ACCESSION   DAOMYJ010000096 DAOMYJ010000000
VERSION     DAOMYJ010000096.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867850
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prosthecobacter sp. (wastewater metagenome)
  ORGANISM  Prosthecobacter sp.
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Verrucomicrobiaceae; Prosthecobacter.
REFERENCE   1  (bases 1 to 17346)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17346)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.30
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:34:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,742
            CDSs (total)                      :: 4,684
            Genes (coding)                    :: 4,676
            CDSs (with protein)               :: 4,676
            Genes (RNA)                       :: 58
            tRNAs                             :: 55
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17346
                     /organism="Prosthecobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1132_length_17346_cov_19.285351"
                     /isolate="AMR_MDS_5629"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1965333"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..378)
                     /locus_tag="PLP58_11430"
     CDS             complement(<1..378)
                     /locus_tag="PLP58_11430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ09175.1"
                     /translation="MKTPPSTDREMTRASWLDDVLRLAGTRAGDGWEALHDALDALAV
                     RTAPVARTTWKWVVAAQGLAVLAPMLWLMRTPFEWSASLVALLSLLAVGALVGILWWV
                     RWRGMQRVWARARLLAEVARAIAS"
     gene            complement(446..1744)
                     /locus_tag="PLP58_11435"
     CDS             complement(446..1744)
                     /locus_tag="PLP58_11435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ09176.1"
                     /translation="MKPPATLFSAVHSPRLLLASAALVCLCSCGTAAYRGTMRGYSRA
                     YAQGNDELMLQNLARLANHQPAHWMQMGAVTASMNFERGLTSSTSRAERSFSLGSALG
                     LTLKADERPTFNFVPLTGKEFADRMMAPISPRVFFTLFEEGWPVDLLMRLLIERIDLG
                     DSYRSHGTLTNQPGNFSTAYLEFLQVCEAARLLQQERQLVLDISSEFSPLAEGHAVAA
                     APSSEDLMKAREKGLVWQKSGTGWVLGEMRERAVFRLRHGMLSAAGRNNVFSKPGLGT
                     AAARLLVGDFFNMLESGLRLKAPDSQERSGGDGTQARLVLRSLGGVMAAAAREQEDFE
                     RILREHGGEFLERTPECQREPVLRLVWQDVPRAELLKASVRLTHAGREYMVTDRKMTD
                     RQMCEAGSRNRDVFRLLMGLYHLVSADMEKLKFPQTLLIQ"
     gene            2105..4930
                     /locus_tag="PLP58_11440"
     CDS             2105..4930
                     /locus_tag="PLP58_11440"
                     /inference="COORDINATES: protein motif:HMM:NF019223.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1549 domain-containing protein"
                     /protein_id="HRJ09177.1"
                     /translation="MTRFCQFSACLLILPAAGAALRADDGARFFENEVRPLLVKHCYE
                     CHSLESGKQKGGLLLDRREGWQTGGDAGPSIVPGDVEKSLLIHSIRYLDEDLQMPPKS
                     RLSDAEVAVLEKWVRIGAPDPRDAALADAVRREGINIEEARRTWAFRPLQNPPVPGVR
                     LTDWPRRDLDAFILAALESQKLSPAPDAAPRALIRRLYYDLTGLPPSSEQVEAFASAP
                     DTQAMLPRIVDQLLNSTAFGEKFGRHWLDLVRYADSNGGDRNYTFYQAWRYRNYVIAA
                     FNEDRPYHEFIREQIAGDLMPWENEIQRRERLVATTFLALGPKMLTERDKEKLNLDTV
                     DEQMDTLGRAFLGLTIGCARCHDHKFDPFTQRDYYAMAGFFRSTQVVMGTRNGCVNVA
                     SWVEQPLPGPGMNDLAAKVARLELTMRLKVERDFMKKAGGKMSLQNLPLAGVLYDEED
                     AELVGEWKTSSLSSNRFGAHYIHDDKKGRGAKKAVFRGSLPETGVYEVRLAFPGKANG
                     ATNTRVIIEAFDGVHELRLDQTKDPRIGGLFEPVGRFRFEKGGRVNVIISNDDANGYV
                     FADAVQFIHENDIEREAAALAMAEGKAAGDPLLMMDSADLAKELNKQIAELKDAELAM
                     TPRDAADAGDVNLRVRGEPGQLGPVVPRNFPVVLHSGPPPAIAPGSSGRLEFARWITC
                     DENALLDRVIVNRVWAWLMGRGIVASVDNFGGQSTGPTHPGLLDHLASKFRATGGSVK
                     ALVREIALSRAYQAAAEADGPRVRADPDNRWFGRRSMRRLTAEEIRDSLLHLSGRLDP
                     QPAAATALTYGEDLDKTMSLDKERRRSVYLPVARNNQLAEMEIFDAANPEMVAGERPL
                     TTVPTQALYLLNSDFMQSQARELGMSAYARAGPVEWLYLTILGRAPGADEKLRAEKFI
                     EAGDDREETVSDLAHALLASTEFLFLE"
     gene            4976..6172
                     /locus_tag="PLP58_11445"
     CDS             4976..6172
                     /locus_tag="PLP58_11445"
                     /inference="COORDINATES: protein motif:HMM:NF019474.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HRJ09178.1"
                     /translation="MRHILPLLLVSLPLQAAEPAKVRLWPQGAPMARGQEDKDQPFVY
                     VWPAPKDKANGAAFVVCPGGGYGGLAADHEGAQVARWLNGLGVTAFVLHYRLGSQGYH
                     YPVQLMDVQRAIRHARSHAGEHGIDPARVGIIGFSAGGHLTSMAATLFDEKPAQMTND
                     AVDQLSARPDVAVPTYPVISMIDEFAHSGSRKNLLGPDNNTDEMARQVSTHLRVTPRT
                     PPVFLFQTDEDTVVPAENAVQFYLACRKHGVPAELHCYRPGPHGVGLYLGDPVLGTWS
                     GHLRDWLRDQGFFKPSPRASVSGTLTVNGTPVSWGSVIFSTQDPAAPVACARVMRGKF
                     KLDSANGPVVGKARLTVSHSAADVAGLDTADGTATTAAEQKPGSGAWVVEIQAGENTL
                     DLQLTR"
     gene            6322..7827
                     /locus_tag="PLP58_11450"
     CDS             6322..7827
                     /locus_tag="PLP58_11450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009959835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase"
                     /protein_id="HRJ09179.1"
                     /translation="MKSLLPFAALFFPCWLEAAPPNVLLILVDDLKPALGCYGDTRAK
                     TPHMDRLAARGMRFDLAYCNQAVCAPSRFNLMLGSRSTSTALYGLGSRLRERLPDAVT
                     MPQHFARHGGHRTESIGKVFHIGHGNEGDPQSFSVPHFKDKVIEYLDPASTQGGQLTR
                     EEALFTNQKLDQIRSLPRGTAFEAPAAGDEDYADGRVAAETVRRLGAAARRRKENGTP
                     FFIAAGFVRPHLPFCAPKSYWDMHDPAKLPQPEFEEHPAGSPLVARKRGGEIAAYDPV
                     PERDDAAFSPELKRRLIHGYYASTSYVDAQIGRVMDALDRLGLAEETLVVLWGDHGFH
                     LGDLGIWTKHTNYEQANRIPLVIIAPGVAKPGSSTRQLAETVDIFPTLAALAGLPAPS
                     GPQPVDGVSLEPVLRDPNARVRDHAFHCFPKQKLGRAIRTERHRLVEWKTPGAAPETA
                     EIELYDYEADPLERENLATQQPETVARLRAILARHPEALPMGPPAKKKKQP"
     gene            7973..9118
                     /locus_tag="PLP58_11455"
     CDS             7973..9118
                     /locus_tag="PLP58_11455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009962465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HRJ09180.1"
                     /translation="MTRYQLLVALLHFIVFFLGAGIGSFLNVVIYRLPRGLSVNEPRR
                     SFCPSCKYQIPWWRNIPVLSWLTLRGKCANCGAPISARYVGVEVLTGLLFYAVFRQFG
                     GDWALAAQWGPQVLCLWVFTALLVAGTFIDIEHFILPHSITLGGTLAGLVCATFVPEL
                     VGQVTHLRGFLFSLGSAALGLGSLWLVVELGKLAFGRKKYRFDKQEAWSVTQPDDTLP
                     PVVTLAGETYSWEDLFMRASDRLVISCARLKTNQSEWNNVTAELWMEKLKVRGPDGKM
                     EEYNLEGVTLLEGTTNEVVIPREAMGFGDVLFLMMIGSFCGWQAVLFTILAASVLGTL
                     VSGGTRLVGLAAWGGKLPFGPYLAAGAMLWVFYGPAAVAWYTSRLHL"
     gene            complement(9197..12250)
                     /locus_tag="PLP58_11460"
     CDS             complement(9197..12250)
                     /locus_tag="PLP58_11460"
                     /inference="COORDINATES: protein motif:HMM:NF019223.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PSD1 and planctomycete cytochrome C
                     domain-containing protein"
                     /protein_id="HRJ09181.1"
                     /translation="MVPRHIILLLLTLPALADDSVSFSHEILPLLSENCLSCHGPDEA
                     QRKADLRLDTREGALEVIVPGRPAESSLLERIVSKDADEVMPPPKSHKPPLTAEQAEL
                     LRRWIAEGAPWGRHWAFEKPVKAPMPASTPEITHPIDRLLSARLEKRGLSLAPPAPPH
                     TLARRMTFALTGLPPAEADAADFSPERLADRLLASPHFGERLAMWWLDAARYSDTDGF
                     QADATRTNWPWRDWVTRAFNDNMAFDEFTRLQFAGDLLPEATAEQKLATCFHRNHMTN
                     GEGGRDPEESRVDYVLDRVNTTGAVWLGLTLGCAQCHSHKFDPVSHADYYRMTAFFNS
                     IDEDGKAGKGAKPYLPYKSPLAPAAVAEARQLVEQRRPLEQAARSEAQVPFEHWLEAQ
                     AARVAGGFNAWHTLKGRLESSEGTLLQQDEEGIVRAGGPNPRQDDYRLAGAVGLARVT
                     GFRLEVLPDAAHTGGGLSRGEKGEFILTNVKAQVLTLGSSQMRELVMSGAVADVSADK
                     KGKKDYGDVKDTLDDDPRNGWTTEGADRAQPHTAVFALKEPWIPQPDEELLIELKHRS
                     TLGDANIGKFRVSLTDQPGEAVRSLARAPLEDLAFTGGDARRVPAALRERLFAQFLED
                     HAPYQEAKAALDRAETQLQEFTAAASSLEVMVLAERKEPRATHVLLRGEWDKHGEKVA
                     PGAPAEIAPWPESARADRLGLAEWIVSRENPLTARVIVNHLWQMLFGAGLVRTPGDFG
                     LQGERPVYPEILDWLAVEFMESGWDVKHIVRLIVTSRAFQQSSSDRPVSLSGDAAAAA
                     QSLTALDPDNRLLARGPRFRHPSWMLRDAALSASGLLNPALGGPPVRPPQPPGVWEEN
                     FMGRFTYVPSEGGARHRRTLYAFWRRSVAPTFLFDSAQRRVCEVAVTRTNTPLQALTL
                     LNDLSYLEAARALAAAAARSAQDEAARLQHLHRRVLTRAAEPRELAVLAREHQRALAH
                     YDMRPDEAAVFLEEKPGSSPDQPARAALAVIASLLLNLDEAVTQE"
     gene            complement(12411..12782)
                     /locus_tag="PLP58_11465"
     CDS             complement(12411..12782)
                     /locus_tag="PLP58_11465"
                     /inference="COORDINATES: protein motif:HMM:NF014035.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HRJ09182.1"
                     /translation="MTTALLLKCLGIGVLSGVVAALCGVGGGVVMVPAFVALLALPQK
                     TAVATSLAIIIPTALMATTQNARHGLVDWRVAVVTAVSASVFAYFGSGWMRAMSNESL
                     TRIFGAVLLVFGARMLWLGKA"
     gene            complement(12803..13987)
                     /locus_tag="PLP58_11470"
     CDS             complement(12803..13987)
                     /locus_tag="PLP58_11470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009965167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-dependent sugar dehydrogenase"
                     /protein_id="HRJ09183.1"
                     /translation="MKSILTAAALSLAFSAQAADTIKLTRIHESLETPFPVALVIPDD
                     GSGREFLALQRGQVLILPKQEGGAAKTFLDLSGREMEAANGKFEEGLNGLAFHPKFKD
                     NGLFYLAYTLQKPKRLVISEMKASTADADKADEGTERVLLEIPLVNWNHHGGNILFGP
                     DGFLYIGVGDTSKRNDELKMAQLNAVLVGKILRIDVDSREYQGRYGIPADNPFADGVN
                     ALPQIYAYGIRNPWGLHFDSQGRFWMADVGQDLWEEINLVVKGGNYGWSFREGARAFP
                     LRTDPAPEGAQLIDPVHEYHHGDGLSITGGITYTGKGAPELAGAYIYGDFVLGKIWAL
                     RLGEDGKVKSNDLLYTSPQTPPSDPKKKPMVHVKPTAFCADARGELIVLDWNGALYRV
                     GK"
     gene            complement(14072..14641)
                     /locus_tag="PLP58_11475"
     CDS             complement(14072..14641)
                     /locus_tag="PLP58_11475"
                     /inference="COORDINATES: protein motif:HMM:NF024635.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HRJ09184.1"
                     /translation="MEPAPAPAIQLTEANWRQTLGELCASLGSVSRPVLLCVCGKPGS
                     GKTTLAKIIRKEGLPGIPPRRVCVIDDGVLSVPVLWFFRKRVRFHSKERDNLKPFQPW
                     LRGRQLVVYVGASPHRRLDRCDVALRIRCPDEERLRRLVHRNEDGEQRFLKTRDVSDE
                     VMIAADHVFDMPALGTEGLAGERGAPAAA"
     gene            14970..>17346
                     /locus_tag="PLP58_11480"
     CDS             14970..>17346
                     /locus_tag="PLP58_11480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ09185.1"
                     /translation="MDFSKSPDATVMNAFDLCILRADADVDLEAAHALGSRIIARINP
                     FEIAAGSDAARAAEVLGIPMFEGVSPGSLRLDATHPHWTRLVTRVLVQKTAVRGFDGV
                     LITGLEGIGQEAERAALLESLNALRAAFPDKILLLDGAFDLAREARRTVDGLLFTGFG
                     NSAGTASARRQEQQVREAARLGMKAYVVGFADPENPGDLDNRARQVRELGGVPFFTTP
                     SMDGVNLGPLREVARRVLVLHSGPVQQTFTARFLHGSLQWLGHEVVYRDIQARESAPQ
                     AHASLRGVIFDHSLAADSGKDLAALVRHLAAAGVPVLLNSLDWLAASGQVLQAELGIE
                     TGGKMPPGLKLRPLPMESAFANPSHAEPAADCRDILAVKAPEDARLVLSLRADDRQTD
                     QVFLAPWGGVWLEPRALEKGTRIQPLSFLEAWLAGAAPAPVADTTSQDGRQLLVCHVG
                     SEGFDAITPRPGLPMAAEVMVDEVLAKYPLPFSVAVCEGDLRGWTPGHAPAEALRREV
                     AARELFSLPNVEPASATLSRPLDWTPGAGITRPLHESASDTRRGMEREVAGSLAWLHQ
                     QLTPGRTGGVPLIVWPEGAQPSREAVTFSRRMGVENAAVWAFEGATGCVLPPRSWSRA
                     DAFQTWLHDPRQGRALDASAIIRHAETLGAERWLAPVQVCLGFADAATDAALAQTRRL
                     LDWCSTRPLHPVSLSAYARLARDAEHSRVFLAGPDHWILVNAGHARTFRMPASAGVPD
                     LERCVGITGYIQHGGQIYIHTLGRQRTELRMIQSPAAQRLRLASSSGAVRWL"
BASE COUNT         2936 a         5835 c         5625 g         2950 t
ORIGIN      
        1 ggatgcgatc gcccgcgcca cctccgccag caggcgcgcc cgcgcccaca cgcgctgcat
       61 gccgcgccag cgcacccacc ataggatgcc gaccagcgcg cccaccgcca gcaggctcag
      121 gagcgcaacc agggacgcgc tccactcgaa gggcgtgcgc atcagccaca gcatcggcgc
      181 cagcaccgcc agaccctgcg ccgccaccac ccatttccag gtcgtcctcg cgaccggagc
      241 ggtgcgtact gccagagcgt ccagcgcatc gtgcaaagcc tcccagccat cccccgcccg
      301 cgttcccgcg agtctgagga catcatcaag ccatgaggcg cgtgtcattt cccggtcggt
      361 ggagggcggc gttttcatga agggtggatg gatggcttgt cttcctcggc atcccgcgca
      421 tggcgcgcca ggaggcgcgc gcattctact ggatgagcag cgtctgcgga aacttcaatt
      481 tttccatgtc ggccgacacc agatgataga ggcccatcag caggcggaag acatcgcggt
      541 tgcgcgagcc tgcctcgcac atctgccggt ccgtcatttt ccggtctgtc accatgtatt
      601 cgcggcccgc atgggtgaga cgcacggagg ctttcagcaa ctccgcgcga ggcacatcct
      661 gccacaccag cctgagtact ggctcccgct ggcattcagg agtccgctcc aggaattcgc
      721 cgccatgctc gcgcagaatg cgctcgaagt cctcctgctc ccgcgccgcc gccgccatca
      781 caccccccag ggagcgcagc accagcctgg cctgtgtgcc atcgcccccg gagcgctcct
      841 gggagtcggg agccttcaga cgcaatccgg actccagcat gttgaagaaa tccccgacca
      901 gcagcctggc ggcggctgtg cccaatcccg gttttgaaaa gacattgttc cggccggcag
      961 cggaaagcat tccatgccgc agccggaaaa ccgcgcgctc gcgcatctct cccagcaccc
     1021 agcccgtgcc actcttctgc caaacgaggc ctttttcccg cgccttcatc aagtcctccg
     1081 aggagggggc cgccgcgact gcgtgcccct cggcgagcgg gctgaactcc gagctgatgt
     1141 ccagcaccag ttggcgctcc tgctgaagca ggcgcgccgc ctcgcacacc tgcaggaact
     1201 ccagataggc ggtggaaaaa ttgcccggct ggttggtcag cgtgccgtgg ctgcggtagg
     1261 agtcgcccag gtcaatgcgc tcgatcagca ggcgcatcag cagatccacc ggccagccct
     1321 cctcgaagag ggtgaaaaac accctgggcg agatcggcgc catcatccgg tcggcgaact
     1381 ccttccccgt cagcggaaca aagttgaagg tcgggcgctc atcggccttc agcgtcagcc
     1441 ccagcgcgct gcccagggaa aacgagcgct ccgcgcggct ggtgctgctg gtgaggccgc
     1501 gctcgaaatt catcgaagcc gtcaccgccc ccatctgcat ccagtgcgcc ggctggtggt
     1561 tcgccagccg cgccaggttt tgcagcatca actcgtcatt gccctgcgcg taagccctgc
     1621 tgtaaccccg catcgtcccg cggtatgccg ccgtgccgca ggagcaaaga caaaccagcg
     1681 cggcggaagc caggagcagg cggggcgaat gaacggcgga aaaaagcgtt gctggcggct
     1741 tcattcagaa tttttgtgtt attcgtgaca gatgacggag ccgacgcagg tccacaagcg
     1801 gcacaaaagg aaaagatgac gcggactcca accagcccaa ggaaacatca ccctggactt
     1861 tgggagtcat gccttggcag aacgcatcaa ccgcatcagg aattccaaga gaaaaaacgc
     1921 cagaggcatc ggcaagcacc ccgagagcga aaattcgctc cggagaggat aagattcatc
     1981 ccggccgtcc gatgaccgga aaaggcatgc tagaaatcca gagcgacctc ccccagccaa
     2041 aaagtcgaat gtgttgctaa aacgcaagat ggcttgcggg cggaacgcta tccttcggtc
     2101 tttcatgacc aggttttgtc aattctcagc atgtcttctc attcttcccg cagcgggcgc
     2161 cgccctgcgg gcggatgatg gcgcgcgctt ctttgaaaac gaggtgcgcc cgctcctggt
     2221 caagcactgt tacgagtgcc actccctaga gtccggcaaa caaaaaggcg ggctgctgct
     2281 cgaccggcgg gaaggctggc agaccggcgg cgacgcgggg ccgtccatcg tgccgggcga
     2341 tgtcgaaaaa agccttctca tccacagcat ccgctatctg gacgaggatc tgcagatgcc
     2401 tcccaagtcg cgcctgagcg acgccgaggt ggccgtgctg gaaaagtggg tgcgcatcgg
     2461 cgcgcccgac ccgcgcgatg ccgcgctggc ggacgccgtg cgcagggagg ggatcaacat
     2521 cgaggaagcc cggcggacct gggccttccg ccccctgcaa aatccgcccg tgcccggagt
     2581 gcgtctcaca gactggccgc gcagggacct cgacgcattc atcctcgccg cgctggagtc
     2641 gcaaaaactg agtcccgcgc ctgacgcggc cccgcgcgcg ttgatccggc ggctttacta
     2701 cgacctcacc ggactgcccc cctcttcgga gcaggtggag gccttcgcca gcgcgccgga
     2761 cacgcaagcc atgctcccgc gcatcgtgga ccaactgctc aacagcaccg cctttggtga
     2821 aaaattcggc cgtcactggc tggacctcgt ccgctacgcc gactcgaacg gcggcgacag
     2881 gaactacacc ttctaccagg cgtggcgcta ccggaactac gtcatcgccg cctttaacga
     2941 ggaccgcccc tatcacgagt tcatccgcga gcagatcgcc ggagacctca tgccctggga
     3001 aaacgaaatc cagcgccgcg agcgcctcgt cgccaccacc ttcctcgcgc tcggcccgaa
     3061 aatgctcacc gagcgcgaca aggagaaact caacctcgac accgtggacg agcagatgga
     3121 cacgctcggc cgcgcctttc tcggtctgac catcggctgc gcccgctgcc atgaccacaa
     3181 gttcgacccc ttcacccagc gcgactatta tgcgatggcc ggttttttcc gctccaccca
     3241 ggtggtcatg ggcacgcgca acggctgcgt caatgtcgca agctgggtgg agcagccgct
     3301 gcccggaccg ggcatgaacg atctggccgc caaagtggcg cgccttgaac tgaccatgcg
     3361 cctcaaagtc gagcgcgact tcatgaagaa agccggcggc aaaatgtcgc tgcaaaacct
     3421 gcccctggcc ggtgtgctct atgatgagga ggacgccgag ctggtaggcg agtggaaaac
     3481 ctcgtcgctt tcctcgaacc ggttcggcgc gcattacatt catgacgaca aaaaaggccg
     3541 cggcgccaag aaggccgtct ttcgcggcag cctgccggag accggcgttt acgaagtgcg
     3601 cctggccttt cccggaaaag ccaacggcgc caccaacacg cgcgtcatca tcgaggcttt
     3661 tgacggcgtt catgagttgc ggctcgacca gacgaaggac ccgcgcatcg gaggtctgtt
     3721 cgagccggtc ggacgcttcc gctttgaaaa aggcgggcgc gtcaatgtca tcatcagcaa
     3781 cgacgatgcc aacggctacg tctttgccga cgccgtgcag ttcattcatg aaaacgacat
     3841 cgagcgcgag gccgcggcgc tcgccatggc cgagggcaag gcggcgggtg atccattgct
     3901 gatgatggac agcgcggatc tggccaaaga gctcaacaag caaatcgccg aactcaagga
     3961 cgcagaactc gccatgaccc cgcgcgacgc cgccgacgcc ggcgatgtga acctgcgcgt
     4021 gcgcggggag ccggggcagc tcggtccggt cgtcccgcgc aacttccccg tcgtgctgca
     4081 cagcgggccg ccgcccgcca tcgcgcccgg ctcaagcggc aggcttgagt tcgcgcgctg
     4141 gatcacctgt gatgaaaacg ccctgctcga ccgtgtcatc gtcaaccgcg tctgggcctg
     4201 gctcatgggc cgcggcatcg tcgccagcgt ggacaacttc ggcggccaaa gcacggggcc
     4261 aacgcatccc ggactgctcg accatctcgc ctccaaattc agggccacag gcggcagcgt
     4321 caaagcgctc gtgcgcgaga tcgcgctgag ccgtgcctac caggccgccg ccgaagcgga
     4381 cgggccgcgc gtgcgcgccg accctgacaa ccgctggttc ggccgccgct ccatgcgacg
     4441 cctcaccgcc gaggaaatcc gtgacagcct gctccatctt tcaggccggc ttgaccccca
     4501 gcccgcagcc gccaccgcgc tgacttatgg cgaggatctc gacaaaacga tgagcctcga
     4561 caaggagcgc cggcgcagcg tttacctgcc tgtcgcgcgc aacaaccagc tcgccgagat
     4621 ggaaattttc gacgcggcca accccgaaat ggtcgccggg gaaaggccgc tgaccaccgt
     4681 gcccacgcag gcgctttacc tgctcaacag cgacttcatg caaagccagg cccgcgaact
     4741 cggcatgagc gcttatgcgc gggccgggcc ggtggagtgg ctgtatctga ccatcctcgg
     4801 acgcgctccc ggcgcggacg agaagctccg cgcggagaaa ttcatcgaag caggagacga
     4861 ccgtgaggag accgtgtccg atctcgccca cgccctgctt gcctccacag agtttctttt
     4921 cctggagtga gggagtggcg cgttcatccc gcttgccaga aacaaatcac ccgccatgcg
     4981 ccacatccta ccgctcctcc tcgtctccct cccgctccag gccgcagaac ccgccaaagt
     5041 gaggctttgg ccgcagggcg cgccgatggc gcgggggcag gaggacaagg accagccttt
     5101 tgtttatgtc tggcccgcgc cgaaggacaa ggcgaacggc gcggcctttg tcgtctgccc
     5161 tggcggcggt tacggagggc tggcggccga ccacgagggc gcgcaagtgg cccgctggct
     5221 caacgggctg ggcgtcaccg ccttcgtgct gcattaccgc ctgggcagcc agggttatca
     5281 ttatcccgtg cagcttatgg atgtgcagcg cgccatccgc catgcgcgct ctcatgccgg
     5341 ggagcacggc attgatcccg cgcgcgtggg catcatcggc ttctcagccg gagggcatct
     5401 gacttccatg gcggccacgc ttttcgacga aaagcccgcg cagatgacga acgatgccgt
     5461 ggaccagctc agcgccaggc cggatgtcgc agtgcccact tatccggtga tctcgatgat
     5521 tgacgagttc gcccattccg gctcgcgcaa aaacctgctc ggtcccgaca acaacaccga
     5581 cgagatggcg cgccaggtga gcacgcatct gcgcgtgaca ccgcggacgc cgccggtctt
     5641 ccttttccag accgatgagg acactgtggt gcccgcggag aacgcggtgc agttttacct
     5701 ggcctgccgc aagcacggcg tgcccgcgga gctgcattgc taccggcccg gcccgcatgg
     5761 agtgggtctt tatctcggtg atcccgtgct cggcacatgg agcgggcatc tgcgcgactg
     5821 gctgcgtgac cagggctttt tcaagccgtc cccgcgcgcg tctgtgagcg gcacactcac
     5881 tgtcaatggc acacccgtga gctggggcag cgtgattttc tcgacgcagg atcccgccgc
     5941 tccggtggcc tgcgcgcgtg tgatgcgcgg gaagttcaag ctcgactccg ccaatggtcc
     6001 ggtggtcgga aaagcgcgcc tcactgtcag ccacagcgcc gccgatgtgg ccggcctgga
     6061 cacagcggac ggcaccgcca ccaccgccgc cgagcaaaaa cccggaagcg gcgcgtgggt
     6121 ggtggaaatc caggcgggtg agaacacgct cgatctgcaa ctgacccgct gacctggact
     6181 cgaacccatg tcttgcgaaa cgggcagcgg gacgcccgcg ccgcatgcgg ccactcgccg
     6241 cgattcatca agttgcactt tcgataggca tgcgtggctc catgcctgca acagcgcgtc
     6301 ccttgtcccg tcttcatggg aatgaaaagt ctcctgccgt tcgccgcttt gtttttcccg
     6361 tgctggcttg aagcggcgcc gccgaatgtg ctgctcatcc ttgtggatga tctgaagccc
     6421 gcgctgggct gttatggcga cacgcgcgcg aagacgccgc acatggaccg cctggccgcg
     6481 cgcgggatgc gctttgacct ggcctactgc aaccaggccg tgtgcgcgcc ctcgcgcttc
     6541 aatctcatgc tcggctcccg ctccacgagc accgcactct acggactcgg cagccggctg
     6601 cgcgagcgtt tgccggacgc ggtgacgatg ccgcagcact tcgcccggca tggcggccac
     6661 cgcacggagt ccatcggcaa ggtctttcat atcggccacg gcaacgaggg cgacccgcaa
     6721 tccttcagcg tgcctcattt caaagacaaa gtcatcgaat acctcgaccc cgccagcact
     6781 cagggcggac aactcacgcg cgaggaggct ctgttcacca accagaagct cgaccagata
     6841 cgaagcctgc cgcgcgggac cgctttcgaa gccccggcgg ccggtgacga ggactatgcc
     6901 gacgggcggg tggctgcgga gacggtcagg cggctgggcg cggcggcgcg gcgccgcaaa
     6961 gaaaatggca cgccattttt catcgccgca ggcttcgtgc gtccgcatct gccattttgc
     7021 gctccgaaat cctactggga catgcacgat ccggcgaagc tgccgcagcc ggagttcgag
     7081 gagcatccgg cgggatctcc cctggtggcg cgcaaacgcg gcggcgagat cgcggcatat
     7141 gatcctgtgc ctgaaagaga tgacgcggcg ttttccccag aactcaagcg caggctcatc
     7201 cacggctatt atgccagcac gagctatgtg gacgcgcaga tcggcagggt gatggacgcg
     7261 ctggaccgcc tcggcctggc ggaggaaact ctcgtcgttt tgtggggcga ccacggcttt
     7321 cacctcggcg atcttggcat ctggaccaag cacaccaact acgagcaggc gaaccgcatc
     7381 cctctcgtca tcatcgcgcc tggagtggcc aaaccaggct ccagcacgcg ccagcttgcg
     7441 gagaccgtgg acatcttccc cacactggcc gcgctggcgg gtcttcccgc gccgtccggt
     7501 cctcagccgg tggatggtgt gagcctcgag ccggtgctgc gcgatccgaa tgcgcgcgtc
     7561 cgcgatcatg ccttccattg ttttccaaaa caaaaactcg gccgcgccat ccgcacggag
     7621 cggcaccgcc ttgtcgagtg gaagactccg ggcgccgcgc ccgaaaccgc cgagatcgaa
     7681 ctctacgatt atgaggccga ccccctggag cgcgaaaatc tcgccaccca gcagccggag
     7741 accgtggcaa ggctgcgcgc catcctcgcg cgccacccgg aagcgctgcc aatggggccg
     7801 ccggcaaaaa agaaaaagca gccgtgacgc ggacactcct gcccgccccg ccttccctgg
     7861 agtcccgcca aatcctggga aacaagaccg ccggtgtcac ggcgcgcagc ccgcacccgc
     7921 cgccattcgc gtcttgccgg gccgcgcgga cggggccaga tttccgcagc gcatgacccg
     7981 ataccagctt ctcgtcgccc tactgcattt catcgtcttc tttcttgggg cgggcatcgg
     8041 ctcgtttctc aatgtggtga tctaccggct gccgcgcgga ctttccgtca atgaaccgcg
     8101 ccgctccttc tgcccttcgt gcaaatacca gatcccctgg tggcgaaaca ttccggtgct
     8161 gagctggctg acgctgcgcg gcaagtgcgc gaactgcggc gcgcccatct ccgcgcgtta
     8221 tgtgggcgtg gaggtgctga ccgggctgct gttttacgca gtgttccggc agttcggcgg
     8281 tgactgggcg ctggcggcgc agtgggggcc gcaggtgctt tgcctgtggg tgttcacggc
     8341 gcttttggtc gcgggcactt tcattgacat tgagcacttc atcctgccgc acagcatcac
     8401 gctgggcggc acgctggcgg gcctggtgtg cgcgactttt gtgccggagc tggtgggcca
     8461 ggtgacgcac ctgcgcggct ttcttttctc gctcggcagc gccgcgctgg gcctgggcag
     8521 cctgtggctg gtggtggagc tgggaaagct ggccttcggc agaaagaaat accgctttga
     8581 caagcaggag gcatggagcg tgacgcaacc ggacgacaca ctgccgccgg tggtgactct
     8641 ggccggggag acctactcgt gggaggattt gtttatgcgc gccagcgacc gtcttgtgat
     8701 ctcctgcgcg agattgaaga ccaatcagag cgagtggaat aatgtgaccg ccgagctgtg
     8761 gatggagaaa ctcaaggtgc gcgggccgga cgggaagatg gaggaataca acctggaggg
     8821 cgtgacgctg ctggagggca ccaccaacga ggtggtgatc ccgcgcgagg cgatgggctt
     8881 tggcgatgtg ctctttctga tgatgatcgg ctctttctgc ggctggcagg ccgtgctttt
     8941 caccatcctg gccgcctcgg tgctgggcac gctggtgtca ggcggcacgc gcctcgtcgg
     9001 actggcggcg tggggtggca agctgccctt cggcccctac ctcgccgccg gagccatgct
     9061 ctgggttttc tacggccccg cggctgtggc gtggtacacg agccggttgc atctgtagcc
     9121 gtcacttctg tgcctggtgc ccgggctttg gcgcaatttt tccgatcggc tgaagcctgt
     9181 gcatcaacac acttccctat tcctgagtca cagcctcgtc gaggttgagc agcaggctgg
     9241 cgatgacggc cagggcggcg cgcgcgggct ggtcggggga ggagccgggc ttttcctcaa
     9301 gaaacacggc ggcctcgtcc ggccgcatgt cataatgcgc caaagcgcgc tgatgctcgc
     9361 gggcaagcac agccagttcc cgcggttccg cggcgcgggt gagcacgcgc cggtgcaggt
     9421 gctgaaggcg cgccgcctca tcctgcgcgg aacgcgcggc ggcggctgcc agggcgcggg
     9481 cggcctccag gtaggaaagg tcattgagca gggtcagcgc ctgcaagggc gtgttggtgc
     9541 gcgtcacggc cacctcgcac actcggcgct gggcgctgtc gaacaaaaag gtaggcgcaa
     9601 cgctcctccg ccagaaggca tacaaggtgc gccggtggcg cgcgccgccc tcgctgggca
     9661 cataagtgaa gcggcccatg aagttctcct cccacacacc cggcggctgc ggcgggcgca
     9721 cgggcggtcc gccgagggcg ggattgagca ggccggacgc gctcaaggct gcatcccgca
     9781 gcatccagct aggatgcctg aaacgcggac cacgggcgag caggcggttg tccggatcca
     9841 gcgcggtgag cgactgcgcg gcagcggcgg catcaccgga caggctcacc ggcctgtcag
     9901 aactggattg ctggaaggcg cggctcgtga cgatgaggcg gacaatgtgt ttcacatccc
     9961 agccgctctc catgaactcc accgccagcc agtcgagaat ttcaggataa accgggcgct
    10021 cgccttgcag gccgaagtct cccggtgtgc gcaccaggcc ggcgccgaag agcatctgcc
    10081 agagatgatt cacgatcacc cgcgcggtga gcggattttc ccgcgacacg atccactccg
    10141 ccaatccgag tcggtcggcg cgcgcgcttt ccggccaggg cgcgatctcc gccggcgctc
    10201 caggggcgac tttttccccg tgcttgtccc actcgccgcg cagcaggaca tgcgtggcgc
    10261 gcggctcctt gcgctcggcg agcaccatca cctccagcga gctggcggcg gcggtgaact
    10321 cctggagttg ggtctccgcg cggtcgagag cggctttggc ttcctgatag ggcgcatggt
    10381 cttcgagaaa ctgcgcgaag agcctctccc gcaaagcggc cgggacgcgc cgcgcatcgc
    10441 cgccggtaaa ggccaggtct tccagcggcg cgcgcgccag gctgcgaacg gcctcgcccg
    10501 gctggtcggt gagggagacg cggaatttgc cgatgttcgc atcccccagt gtggagcggt
    10561 gcttgagttc gatgagcagt tcctcgtccg gctgcggaat ccacggctcc ttcaaggcaa
    10621 aaaccgccgt gtgcggctgc gcgcggtccg cgccctcggt ggtccagccg ttgcgtggat
    10681 cgtcatccag ggtgtccttc acatcgccat agtctttttt gcccttcttg tccgcgctga
    10741 catcggccac cgcgccgctc atcaccagct cccgcatctg cgagctgccg agcgtgagaa
    10801 cctgcgcctt cacgttggtc aggatgaatt cgcccttctc cccgcgtgaa agaccgccgc
    10861 cggtgtgggc cgcgtccggc agcacctcca ggcggaagcc ggtgacgcgt gccagcccca
    10921 ccgcgccggc caggcggtag tcgtcctggc gcgggttcgg cccgcccgcg cgcacgatcc
    10981 cctcctcgtc ctgctgcaaa agcgtgccct cgctggattc cagcctccct ttgagcgtgt
    11041 gccaggcatt gaagccgccg gcgacacgcg ccgcctgcgc ctccagccag tgctcgaatg
    11101 gcacctgcgc ctcgctccgc gctgcctgct ccaaagggcg gcgctgctcc acaagctggc
    11161 gcgcctccgc cacggctgcg ggtgcgagcg gcgacttgta ggggaggtag ggtttggccc
    11221 ctttccccgc cttcccatcc tcgtcaatgc tgttgaagaa agccgtcatc cggtagtagt
    11281 ccgcatggct cacgggatcg aacttgtgcg agtggcactg cgcgcagccg agtgtgaggc
    11341 cgagccagac cgcgcctgtc gtgttcacgc ggtccagcac ataatccacg cgcgactcct
    11401 ccggatcgcg cccgccctcg ccgttggtca tgtggttgcg gtggaagcat gtcgccagct
    11461 tttgctccgc cgtcgcctcc ggcagcaggt cgcccgcgaa ctggaggcgg gtgaactcgt
    11521 caaaggccat gttgtcattg aacgcgcgcg tcacccagtc acgccagggc cagttcgtgc
    11581 gcgtggcatc cgcctggaag ccgtccgtgt ccgagtagcg cgccgcgtcc agccaccaca
    11641 tcgccaggcg ctcgccgaaa tgaggcgagg ccagcaggcg gtcggccagg cgctccggtg
    11701 aaaaatccgc cgcgtcagcc tctgcggggg gcagccctgt gagggcgaaa gtcatgcgcc
    11761 gcgccagcgt gtgcggaggc gcgggcggcg ccagggaaag gccgcgcttt tccagccgcg
    11821 ccgagagcag ccggtctatg gggtgggtga tttccggcgt ggacgcgggc atgggcgcct
    11881 tcaccggctt ctcgaacgcc caatgcctgc cccagggcgc tccctcggcg atccagcgcc
    11941 gcagcagctc cgcctgctcc gccgtcagcg gcggcttgtg cgacttcggc ggcggcatca
    12001 cctcatccgc gtccttggag acgatgcgct ccagcaggct gctttccgca ggacggcccg
    12061 gcacgatcac ctccagcgcg ccctcacgcg tgtccagacg caggtcagcc ttgcgctgcg
    12121 cctcgtcagg cccgtggcaa ctcaggcagt tctccgagag caagggcagg atctcgtggc
    12181 tgaagctcac gctgtcatcc gccaaagcgg gcagtgttag cagtagcaaa atgatgtgac
    12241 gcgggaccat cggagaaaaa aacagcccca ccataacgcg cccttcaata tccgggttgc
    12301 cgccttgggc atgggggatt ctgctttgaa tgaggcggac gatacttgat tgatgcccgc
    12361 cggttatcga gggccactgc catccttgag cacccggttt cacagggcgc tcacgccttc
    12421 cccagccaca acatgcgcgc gccgaagacg agcaggacgg cgccgaagat gcgggtgagg
    12481 gattcattgc tcatggcgcg catccagccg gagccgaagt aggcgaagac ggaggccgag
    12541 accgcggtga cgacggccac acgccagtcc acgaggccgt ggcgggcgtt ctgcgtggtg
    12601 gccatgaggg cggtggggat gatgatggcc aggctggtgg ccacggcggt cttttgcggc
    12661 agcgccagca gggcgacgaa ggcgggcacc atgaccacgc cgccgccgac gccgcagagg
    12721 gcggccacga cgccgctgag gacgccgatg ccgaggcact tgagcaggag ggcagtggtc
    12781 acggtccggg tgcgggctca attcatttcc ccacgcggta aagggcgccg ttccagtcga
    12841 gcacgatgag ctcgccccgg gcgtcggcgc agaaggcggt gggcttcaca tgcaccatgg
    12901 gctttttctt cgggtcggag ggcggggtct gcgggctggt gtagaggagg tcgttgctct
    12961 tcaccttgcc gtcctcgccg aggcgcagcg cccagatttt gcccagcacg aagtcgccgt
    13021 aaatgtaggc tccggccagc tcgggcgcgc ccttgccggt gtatgtgatg ccgccggtga
    13081 tgctgaggcc gtcgccgtgg tggtattcat gcacggggtc aatgagttgg gcgccctccg
    13141 gggcggggtc cgtgcgcagg gggaaggcgc gcgcgccctc gcggaagctc cagccgtagt
    13201 tgccgccttt gacgacgagg ttgatctcct cccaaaggtc ctgccccacg tcagccatcc
    13261 agaagcggcc ctgggagtcg aagtgcagcc cccaggggtt gcggatgccg taggcgtaga
    13321 tctgcggcaa ggcgttcacg ccgtcggcga aggggttgtc ggccgggatg ccgtagcggc
    13381 cttggtactc gcggctgtcc acgtctatgc ggaggatttt gccgaccagg acggcgttga
    13441 gctgggccat cttcagctcg tcattgcgct tcgaggtgtc gcccacgccg atgtagagga
    13501 agccgtcggg gccgaagagg atgttgccgc cgtggtggtt ccagttcacg agggggatct
    13561 ccagaagcac gcgctcggtg ccctcgtccg ccttgtcggc gtcggcggtg ctggccttca
    13621 tctcgctgat gacgaggcgc ttcggcttct ggagggtgta ggccaggtag aagaggccgt
    13681 tgtccttgaa cttcgggtga aaggccaggc cgttgagccc ttcctcgaac ttgccgttcg
    13741 ccgcctccat ctcgcgcccg ctgaggtcga ggaaagtttt ggccgcgcca ccctcctgct
    13801 tcggcaggat gagaacttgc ccccgctgca gcgccaggaa ctcgcgcccg ctgccgtcgt
    13861 ccgggatgac cagcgccacg gggaagggcg tctccaggga ttcatggatg cgggtgagct
    13921 tgatggtgtc tgcggcctgg gcactgaagg ccagggagag cgcggcggcg gtgaggatgg
    13981 atttcatgcg ttggggatgg aaatagggcg ttcgcccgga aatggaaagt ctcaaacgcg
    14041 gaccggcgcg tttttccttc gtccccatgc ctcatgccgc ggcaggcgcg ccgcgctccc
    14101 ccgccagccc ctccgtgccc agggccggca tgtcgaagac gtgatccgcg gcgatcatca
    14161 cctcgtccga cacatcccgc gtcttcagga agcgctgctc gccgtcctcg ttgcggtgga
    14221 cgagccggcg cagccgctcc tcatccgggc agcggatgcg cagggccaca tcgcaccggt
    14281 ccagccggcg gtgcggggag gcgcccacat agaccacgag ctgtcggccg cgcagccagg
    14341 gctggaaggg cttcagattg tcccgctcct tcgagtgaaa gcgcacccgc ttgcggaaaa
    14401 accagagcac cggcacggag agcacgccgt cgtcaatcac gcacacccgc cggggcggga
    14461 tgccgggcag gccctccttg cggatgatct tcgccagggt cgtcttgccc gagccgggct
    14521 tgccgcacac gcacagcagc accggccggc tgacgctgcc caggctggcg cacaattcgc
    14581 cgagcgtctg ccgccagttg gcctcggtga gctggatggc aggtgcgggg gctggttcca
    14641 tgtgacgatt ctgccaccca tgcaccccct gtcaattcta gcagactctt ccagcgcaag
    14701 aggtgctgcc agggaaaagc gcgcgccaga aaggccgccc atgtcatttc agggtggcta
    14761 tggcagcggg cgaaggaggt aaggagtttg taaggctcct ctttttgatt gttttcataa
    14821 ttttgagaca ctaaaatgcg tctgttctca aatttgataa tattatgccc gaaacccacc
    14881 gatttttccc agcttccctg cttcttggct gcgcaggtct tttgctgtca gggacggcgc
    14941 tccaggcctc ccaagaggca agattgttcg tggatttttc caaaagcccg gacgccacag
    15001 tgatgaatgc ctttgatctc tgcattctcc gtgcggatgc ggatgtggac ctggaggcgg
    15061 cccatgccct cggcagccgg atcatcgcca ggatcaatcc cttcgaaatc gccgccggtt
    15121 cggatgcggc gcgggctgcg gaagtgctcg gcatacccat gttcgaaggc gtctcgcccg
    15181 gtagtctgcg gctggatgcc acccatcctc actggacgcg cctggtgacg cgggttctgg
    15241 tgcaaaagac ggcggtgcgc ggtttcgacg gagtgctcat cacggggctg gagggtatcg
    15301 gtcaggaagc ggagcgcgcg gcgcttctgg agtcgctcaa cgcgctgcgg gcggcgttcc
    15361 ctgacaaaat cctcctgctc gacggcgcct tcgatctcgc ccgcgaggcc cgccggacag
    15421 tggacggcct gcttttcacc ggctttggaa actccgccgg cacagcaagc gcgcgccgcc
    15481 aggagcagca ggtgcgcgaa gcggcgcgtc tgggcatgaa agcgtatgtc gtgggtttcg
    15541 ccgaccctga aaatcccggc gatctcgaca accgcgcgcg gcaggtccgc gaactgggcg
    15601 gcgtgccctt tttcaccacc ccgtccatgg acggcgtcaa tctcggcccg ctgcgcgagg
    15661 tggcgcgccg ggtgctcgtg ctgcattccg ggccggtgca gcagaccttc acagcgcgct
    15721 tcctgcacgg ctcgctgcaa tggctcggcc acgaggtcgt ctatcgcgac attcaggcgc
    15781 gggagtccgc gccgcaggcc catgcctcgt tgcgcggggt gatttttgac cactcgctgg
    15841 ctgctgactc cggcaaagac ctcgccgccc tcgtccgcca cctcgccgcc gcaggcgtgc
    15901 ccgtgctgtt gaacagcctg gactggctgg ccgcctccgg acaggttttg caggcggaac
    15961 ttggcatcga gaccggagga aaaatgcccc ccggattgaa gctccgcccg ctgcccatgg
    16021 agagcgcgtt cgccaatccc agccatgcgg agccggccgc tgactgtcgt gacattctgg
    16081 cggtcaaagc cccggaggac gcccgcctcg tgctctccct ccgggctgac gaccggcaaa
    16141 ccgaccaggt tttcctcgcc ccatggggcg gtgtctggct ggagccccgg gcactggaaa
    16201 aaggcacccg gatccagccg ctcagcttcc tggaagcatg gcttgccgga gccgcgccgg
    16261 cgcccgtggc ggacaccaca agccaggatg gccgccaact gctggtctgc cacgtggggt
    16321 cggagggttt cgacgccatc accccgcgtc ccggcctgcc gatggccgcc gaggtcatgg
    16381 tggacgaggt cctcgccaaa tatccgctgc ctttcagcgt ggcggtctgc gagggcgacc
    16441 tgcgcggctg gacccccgga cacgcgccgg cggaggcgct ccggcgcgag gtggccgcgc
    16501 gagagctttt ttccctcccg aacgtcgagc cggcctcggc aacccttagc cgcccgctcg
    16561 actggacccc aggcgccggc atcacccggc ctctgcacga atccgccagc gacacccgcc
    16621 gtggcatgga gcgcgaagtg gccggctccc tggcctggct gcatcagcaa ctgacacccg
    16681 gcaggacggg cggcgtgcct ttgatcgtct ggccggaggg ggcgcagccc tcccgcgagg
    16741 cggtgacctt ttcccgccgc atgggggtgg agaatgccgc cgtttgggcc tttgaaggcg
    16801 ccacaggctg cgtgctgccg ccgcgttcct ggagcagggc ggatgcgttt caaacctggc
    16861 tgcacgaccc gcgccagggc cgcgcgctgg acgccagcgc gatcatccgg cacgccgaaa
    16921 ccctgggcgc cgaacgctgg ctggcgcccg tgcaggtttg cctgggcttt gccgatgccg
    16981 ccactgacgc ggccctggcg cagacgcgcc ggctgctcga ctggtgctcc acccgtccgc
    17041 tccatcccgt cagcctctcc gcctacgcgc ggctggcgcg tgacgcggag cacagccgtg
    17101 tctttctggc cgggccggac cactggatcc tcgtcaatgc ggggcatgcg cgcaccttcc
    17161 gcatgcccgc cagcgccggc gtgccggacc tggagcgctg tgtgggaatc accggctaca
    17221 tccagcatgg cgggcagatt tacatccaca ccctgggccg ccagcgcacg gagctgcgca
    17281 tgattcaatc ccccgccgcc cagcgcctgc gcctggccag ctccagcggg gcggtgcgct
    17341 ggctgg
//
LOCUS       DAOMYJ010000097        17177 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Prosthecobacter sp. isolate AMR_MDS_5629
            NODE_1144_length_17177_cov_21.340206, whole genome shotgun
            sequence.
ACCESSION   DAOMYJ010000097 DAOMYJ010000000
VERSION     DAOMYJ010000097.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867850
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prosthecobacter sp. (wastewater metagenome)
  ORGANISM  Prosthecobacter sp.
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Verrucomicrobiaceae; Prosthecobacter.
REFERENCE   1  (bases 1 to 17177)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17177)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.30
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:34:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,742
            CDSs (total)                      :: 4,684
            Genes (coding)                    :: 4,676
            CDSs (with protein)               :: 4,676
            Genes (RNA)                       :: 58
            tRNAs                             :: 55
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17177
                     /organism="Prosthecobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1144_length_17177_cov_21.340206"
                     /isolate="AMR_MDS_5629"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1965333"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..349
                     /locus_tag="PLP58_11485"
     CDS             <1..349
                     /locus_tag="PLP58_11485"
                     /inference="COORDINATES: protein motif:HMM:NF012382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HRJ09186.1"
                     /translation="EWARGAHGDEARARLWRNIRRLARGLGIGEESAASAILPVILGD
                     EAAAMKASAQLLEQGFLVPAIRYPTVARGSARLRITLSALHEENEVEALCDAVRFLVP
                     PSERAAGSAASRR"
     gene            complement(338..841)
                     /locus_tag="PLP58_11490"
     CDS             complement(338..841)
                     /locus_tag="PLP58_11490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009964415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ09187.1"
                     /translation="MFPALRRLVLLTAALLVSSCQTTDPRTQQLIEYRRLEIAAETPG
                     NYFIGRRFHIPNTHFWGYVRRPREPWAASRLVMVNERFMRQPDRLPEIPSGDGMAYGS
                     DHNREYRLWGYLTGRRVYDPNSNMALPEFMLQRYEVKSYSPGWLFQPKERFNGSQLLR
                     AEPGSTP"
     gene            complement(911..2104)
                     /gene="lepB"
                     /locus_tag="PLP58_11495"
     CDS             complement(911..2104)
                     /gene="lepB"
                     /locus_tag="PLP58_11495"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: protein motif:HMM:TIGR02227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HRJ09188.1"
                     /translation="MFFLTPRYIKHARQLHKGVTRFIDYKRDLLAPAKLEQICGLRAE
                     LEAAMRQRDKKRIESLHEEINRVCEKALPHAPHSELGENIEVFFVAIVIALGIRGYIA
                     QPFQIPTGSMQPTLNGITAQASAEDPRPGPIGWLTSFLTSTRHIHVVSDHTGYLRDRE
                     PLTEHRFLIFMPYCKLHFRDGKTIRIMAPQRQLMDELQLGAHVNSIPVEVTDDTPDVR
                     RVYGLRGGEHIKKGQLLASGWVQNGDHVLVDKFSYHFRTPVRGEVFVFTTKHIEGIRV
                     PEEQGSQHYIKRLAGVPGDKLEVAPPKLLINGQEAGEPGFQKVMRGTYEQPVEGYRGY
                     SDPRVVGGRIDRIELGNEQYFAMGDNSYNSSDSRAWGPVPERNLVGPAMLCYWPLTRH
                     WGVIR"
     gene            2214..2924
                     /locus_tag="PLP58_11500"
     CDS             2214..2924
                     /locus_tag="PLP58_11500"
                     /inference="COORDINATES: protein motif:HMM:NF018028.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HRJ09189.1"
                     /translation="MPQYHIARDGQQIGVHSEQDVLSGLASGSFRESDLCWTEGMNGW
                     EKLGQRLPAIGAVTVTDNPYAPPAAVHLKAGPPAEYKLASLGLRFGAAMLDTLISMVL
                     LGVPYLFLIVQAGGFEEQSPDLQFTPGVIAAITALVVFGLGLLGVNIYLLATRGQSLG
                     KMWLGIRIVTHPDAEPPGFVKAFLLRAFVNGLIGGVPCLGAIYSLVDICFIFGEERRC
                     IHDLIAGTQVIEGQPPKK"
     gene            2938..3441
                     /locus_tag="PLP58_11505"
     CDS             2938..3441
                     /locus_tag="PLP58_11505"
                     /inference="COORDINATES: protein motif:HMM:NF022281.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2752 domain-containing protein"
                     /protein_id="HRJ09190.1"
                     /translation="MRLIRPFLRPWLAMLIRQRSVCRALLLMLAVIGAGYLLGLPLWP
                     CVFADVTGLPCPGCGMTRAASALLRGEWAEAWRFHPFAFAFAVIGLFAAAGAMLPRAS
                     AGALAAKVEAWEVKSRLTAIFLAVLLIFSLMRVLGFWYQPPLSRPSLFFLIQEMQGGG
                     GLRHQTE"
     gene            3459..3848
                     /locus_tag="PLP58_11510"
     CDS             3459..3848
                     /locus_tag="PLP58_11510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ09191.1"
                     /translation="MEPTSTAAAATGVGLASLAAFGVIFIINLVCWIKVLIALFKKEG
                     AGLGILGILCSIYAYIWGWMKSTELGLKKTMVWWTIAILASIVLSFVSSAAMVGVMAS
                     NPEFQKAMQEAQEKAQQLQQEQPAPQQ"
     gene            complement(3924..4643)
                     /locus_tag="PLP58_11515"
     CDS             complement(3924..4643)
                     /locus_tag="PLP58_11515"
                     /inference="COORDINATES: protein motif:HMM:NF015679.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HRJ09192.1"
                     /translation="MLISIYSISPGINAQIPASTRQLIVARAPAWTSQKAALELWQRE
                     SARAPWQKSSAQPVQVLLGRAGMAWGRGVFTPPRSSTQMKVEKDWRAPAGVFQLGSLF
                     GYAERPPSGARWPYIQVGPWDAYVDDPSNPFYNQHVRVDPRAVPPWFEKQRMRLGDAA
                     YKWMLEIRHNQGPWMQPGAGSAIFFHVRRGPDKPSAGCTTMALPDLVRLVRWVDPKAC
                     PHYVLLPETEYAALRKAWRLP"
     gene            4954..5577
                     /locus_tag="PLP58_11520"
     CDS             4954..5577
                     /locus_tag="PLP58_11520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ09193.1"
                     /translation="MKLLSISFLAALVLSSCATTSEVSLDYVPSTGQIRPGRPDFSVR
                     DFGDRRGVGTHELGTVRTQLGTPVEHVRTRVPAHQIVTNAFAHGLDARGMLSQPGGAR
                     FIVTGEVLDLYCQMLVRPYGYARVRVMLLDAATGQVLHTSVFKGERQGPAYIPGSGSP
                     VPLLRDLVSGALQDAVDRALDDPVIRNRAEGRIQDWPGRSGYEPGML"
     gene            complement(5578..6891)
                     /locus_tag="PLP58_11525"
     CDS             complement(5578..6891)
                     /locus_tag="PLP58_11525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009960000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HRJ09194.1"
                     /translation="MTSRRAFLTSATAASLAMSRLPVIGATGKKYRVALIGSGWWGMN
                     LLREAMASGRVKVTALCDVDANVLENSIEDVKSESGDEPKGYKDYRELLAGAKPEIVI
                     IATPDHWHALQTIAACQAGAHVLVEKPTGHTVNESRAMVKAARDAGVVVQVGLHRRIG
                     PHHVEAMNFLRSGKAGKIGMVRLFVAGRGGVETPKPNSRAPDHMDWDMWCGPAPVRPF
                     NTKLHPGGWRNFLDYANGTMGDWGVHWLDQVLWWSGEKGPKKVFCTGGRPIAGAPVLN
                     DQEQTTDAPDHQVATFEFEQFTAVWEHRKFSDNNNEKHKIGAFFYGENGVLHIGWRDG
                     WTYYPADAKKAVEHGDHQLQEPDGHNIALLWADLLDAIENKRAPVADIESAHRSSCLP
                     MLGMLSWKAGRSIQWDAEKQRIIGDAEASRLLSREYRAPWQYPQV"
     gene            complement(7002..8426)
                     /locus_tag="PLP58_11530"
     CDS             complement(7002..8426)
                     /locus_tag="PLP58_11530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009962811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1501 domain-containing protein"
                     /protein_id="HRJ09195.1"
                     /translation="MSSIIQPPSLVRCAGPLTRRSFMQFGLSGMATLSWPGLLKLRAE
                     NALKPKSERKSIIMVWLPGGQSHIDTYDPKPDASSEYRGPFKTISTKVPGTIFTELLP
                     MNAKIADKFTIVRSMNQSAGGHPAGTMQMFSGDKDTRDKPKPRLPDWMSVAHYLRAKE
                     GGRANPLPNYIGVPAASPEYSSPAYLGDAYAPFAVSDDPNRPNFQVPNIGLDDEAETR
                     RLSDRLALRRSLDKMERAFDREGELDALDEFEAQAATLLTNPKTRDAFDLSKEDPATR
                     DRYGRNRWGQQLLLARRLVEAGVEIVTSSLSGPLCGRVNNWDDHAVNQHQFEALRFRM
                     PTYDRCVSALIEDIYARGLDKKVLVVVTGEFGRTPKISFDRSTGAGDASGPTGTLQPG
                     RDHWPRAFTNVWAGGGIQTGGVIGASDKRGEDVVERPCNAADFLATIYHHLGIDYQKV
                     TLNDLNGRPVHIVENGRAIPELIA"
     gene            complement(8798..9124)
                     /locus_tag="PLP58_11535"
     CDS             complement(8798..9124)
                     /locus_tag="PLP58_11535"
                     /inference="COORDINATES: protein motif:HMM:NF014505.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HRJ09196.1"
                     /translation="MKPWDIYTYEFEGAGAHPAVIVSHPDRVAKADWINVLLCTTQRA
                     NRPPKETEVRLNGADGLDWETLCRCDALWLVEKSKLKGKRGTVAQVRRRQIVNKINAA
                     MGWKLV"
     gene            complement(9121..9351)
                     /locus_tag="PLP58_11540"
     CDS             complement(9121..9351)
                     /locus_tag="PLP58_11540"
                     /inference="COORDINATES: protein motif:HMM:TIGR02606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051983 - regulation of chromosome
                     segregation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system ParD family
                     antitoxin"
                     /protein_id="HRJ09197.1"
                     /translation="MNVALTHHFEDFIKQLVTSGRYNNASEVVRAALRKLQESEGESF
                     PPGSLAHLYTTQSSREEGKLARKQRIPRPDEV"
     gene            complement(9447..9875)
                     /locus_tag="PLP58_11545"
     CDS             complement(9447..9875)
                     /locus_tag="PLP58_11545"
                     /inference="COORDINATES: protein motif:HMM:NF012618.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ09198.1"
                     /translation="MRVTEIFTGRQLNLLEGHTHHVTAVAFRSDGRVLASAGAEGTVV
                     TWDMIIGERKRKIEGWAKEVTSLQFIGATNQIFTSAGDNRVRIVNDEGNEIRNIPDLP
                     DYMQAAVSTPNGTTLVAGGEDSLLRIWDSTGKALASFGAK"
     gene            complement(10196..10345)
                     /locus_tag="PLP58_11550"
     CDS             complement(10196..10345)
                     /locus_tag="PLP58_11550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system ParD family
                     antitoxin"
                     /protein_id="HRJ09199.1"
                     /translation="MRRPCGEALSITSGRHNNASEVVRAALRKVQESKGESFPPGSLA
                     HLHIT"
     gene            complement(10342..10938)
                     /locus_tag="PLP58_11555"
     CDS             complement(10342..10938)
                     /locus_tag="PLP58_11555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ09200.1"
                     /translation="MHRVTLPLVILLASCSSSEQDRASKREATPLMSQNMEQRFAASR
                     KRMADPNDRSRFDPGVQSTLARNKGSGSRITSQSYKPGAFKGSKSYSVTPGYKTTDYS
                     GKDKQSGMSGQSFARRDQVASESDDAFAAGESRLASKTARQAGQTFRDAGDTFGTSAN
                     RDVLRSQEKNNRPVFIELEENRRNPAYSEEQVRRLLGR"
     gene            complement(11000..11368)
                     /locus_tag="PLP58_11560"
     CDS             complement(11000..11368)
                     /locus_tag="PLP58_11560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ09201.1"
                     /translation="MHEAVLDLVPVSIDYSVEELGLLAELLVAFARDGHAGGTVFLQS
                     LAEKKTVEAFVPTKWMSLKLGGKVSTGGKLVIDGTTRISGTWPVDLGIFSGSNGWNAF
                     KFDGSSGYSFPSAPPKSPLW"
     gene            complement(11513..>17177)
                     /locus_tag="PLP58_11565"
     CDS             complement(11513..>17177)
                     /locus_tag="PLP58_11565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ09202.1"
                     /translation="AWEKAASLRPGDFTLARQVAELLLRAGLVAEAAVFYEKLAAQSD
                     PARRLDALHDLARIHELGDDFDKAGAALRQALTVLDFRDGRYVEFFRRLVRLHERAGL
                     LDEMRTRLEDAAGSRPPEERSLLDLARFYEFTVDQDAHVAALRALVKAVPQVMDYRWQ
                     LVRALLDHDGAREAAELLDKSMSGDSQAPALVFLRCEADLRLGDAAAAGARLQKLLAA
                     GADTDLEKQALAFAQERSLDTVIEEILRRRVERDPSRQEAVFELAAFHRARKNTAAAD
                     KLLRGFAAGAANEAERGRRLNDVSGFLAGGQDLDSAIILARELVARPDAGREQWLRLA
                     DLLAEQAEAEESAEWLEKAWAASATDEERVEVDERLFSILLGGRKTEAPARAGTAGEF
                     QLPDAFTGKGFASGGSSPQEKDPLPEAVVTRTRACLAAAEAPDASDATLFRAAWWALR
                     TAQRDAAYPFLNRLQKDAATGQPRALSLEAEKLLLELALSDQNRLLAMRVLRRLIERD
                     EPGRTRHVLRLSELMMAAEQERLATLAMNHKGWQLHGSLPPPAQEATRFLEDAWRRHP
                     DSEQILSALSQCYTLQRRHNDATALWKEAIRRAGDGSAIPLMERFAELLMRQHRQQEY
                     IEVQTAILEKEPGVKRRREIYRRFLDRLLWTENGGELPVEEMKKRLELLEKNLATLAR
                     KHPFDGFYHEALALVHERQGGHDLAFKAMKQAYYTAPDTPFSAAQLRDAALRVQDMAS
                     AIYFQKQIAATAPPAELAGESRRLVEMLEQTFQIAEADRVRRRLESRFSQDAAALESL
                     AGHYRATGQDEAERRVYEQIARVRPWDARSLFRIALKSLRLADDTAAGAQLREILDKT
                     AGQSHSPRALHPERTPLPVSDLRKDGAPGPASDVADLAGSAPGITHEETSRLRLFLNL
                     PRPEFAVLPDEVENIRLRAIEELARLRKRGGDTAALDAWVKEWSAAALPAIERLWALY
                     YCGAGPEFRALLVQVLGAPPSLEARFALVWLLLRSHGMEDALAWAGQTGLDITAQEAR
                     RRLLHACAAMLAADNAFRYSSGNLATLGASRLLPNSAVLEITRSLQDRQRYTEALELG
                     ESLRKNSPGLAADYAYFLSRIAESAERWDLAREYLGRVVHGPVRPGPYRGTYDPYIYG
                     LATASRLAMSVQEREETLNTAWKHLQSTPDSAMTRVRKTVVAGLAGAEGRAASELEDL
                     VTGDFLAARQMGEMRGILMPQGSNRHEEPMHLRSFWEETREIQAVLVQQGLAGVVQEA
                     GGRLDERLGAMTLSSRSGFEFGEWRLGQLMRRLRLSDHPTRLRLIREHLASVDMRMEV
                     SVDTLSELGGRLESNGMAREAVEVYRLLPGRAPANPEYALWLIRASEAARDTPTGLAF
                     TLQLLQAEPPMKPPQPGDEVLREKHAHFLAQDYDLKELRRLGGKPQAGRILPGRIPHS
                     VPYLRELALLCERLGREDEALNAWENLLLAFQTSLGEESPPPDPEACLHKARLLQKQS
                     LPVAALATLEQVDLGGKLNPFWRDILHLRAELLAQSGGWEAFRPLMALAVDKKTLASI
                     IHLAGLLSRHERGTEALNYLIQAERVLKDDADRFRLRFEQLRLHTADPAWTPDLGRSQ
                     VASMFRVRSRDRETLGLFLDWMNAQFHGPNRDAWTTLLRSEARAGLDRPLAALALSAF
                     AKDLPESAAADLADGWSAAREGDHTCLELGAETLLDSGRPRWAWHACQTLKDLPTLRI
                     EGRKRPLMARVAHAMGDHTAVRELFAEVVRKSFPGGSQPVEWARAFAQIGERGLAREL
                     YQAAIERNDGSHLLHTALSAAWARFLIEERDFPAAEVFLLRQHWTLPADSAALIFELY
                     QAWDRLEHLRAELPKYHLPRGTEKEVLFNAWQAVKTESLSPVLSD"
BASE COUNT         2762 a         5252 c         5895 g         3268 t
ORIGIN      
        1 ggagtgggcg cgcggcgcgc atggagacga ggcccgcgcc cggctgtggc ggaacatccg
       61 ccgcctcgcc cgcgggctgg gaatcgggga ggaatccgcc gcgtctgcca tcctgcccgt
      121 cattctgggt gacgaggcag cggcgatgaa agcgtcggcg cagttgctgg agcagggttt
      181 tctggtgccg gccatccgct atcccaccgt ggcgcgcggg tcggcgcggc tgcggatcac
      241 gctgtccgcg ctgcatgagg aaaacgaggt cgaggcgctc tgcgatgcgg tacgcttttt
      301 ggtcccgccc agtgagcggg cggcaggctc cgcagcctca aggcgttgag cccggctcgg
      361 cgcgcaggag ctggctgccg ttgaatcgct ccttgggctg gaaaagccag cctggcgagt
      421 aactcttcac ctcgtaacgc tggagcatga attcaggaag ggccatgttg ctgttcgggt
      481 catagacgcg gcgtccggtg aggtaacccc agagccggta ttcgcggttg tggtcgctgc
      541 cgtaggccat gccgtcgccg gaggggattt cgggaagacg gtcgggctgg cgcatgaagc
      601 gctcgttgac catgacgagg cggctggcgg cccagggttc gcgcggacgg cgcacataac
      661 cccagaaatg cgtgttcggg atgtggaagc ggcggccgat gaagtagttc cccggggttt
      721 cggcggcgat ctcaaggcgc cggtattcga tgagctgctg ggtgcgcgga tcggtcgtct
      781 ggcaggagct gaccaggagg gcggcggtga gaaggacaag gcggcgcagg gcgggaaaca
      841 tgcgcggatg atgggaggga gggcgcgggc gcgcaaatga aagctggcgc gcgggaaggc
      901 gcgccatttt tcaccggatc acgccccagt gtctcgtcag cggccagtag cagagcatgg
      961 ccggccccac gagattgcgc tcaggcaccg gaccccatgc gcgcgagtcg ctgctgttgt
     1021 agctgttgtc gcccatcgca aaatactgct cgtttccaag ttcgatgcgg tcaattctgc
     1081 cgcccaccac gcgcgggtct gagtagccgc ggtagccttc cacaggctgc tcataggtgc
     1141 cccgcatcac tttctggaat ccaggctctc cggcctcttg tccattgatc agcagcttgg
     1201 gcggggccac ttccagcttg tctcccggca cgcctgccag gcgcttgatg tagtgctggg
     1261 agccctgctc ctccggcacg cggatgccct cgatgtgctt ggtggtgaag acaaacacct
     1321 ccccgcgcac gggggtgcgg aagtgatagg aaaacttgtc caccaggaca tggtcgccgt
     1381 tttgcaccca gccgctggcg agcaactgcc cttttttaat gtgctcgccg ccgcgcaggc
     1441 catagacgcg ccgcacgtcc ggcgtgtcat cggtgacctc cacagggatg gagttcacat
     1501 gcgcgccgag ctggagctcg tccatcaact gccgctgcgg cgccatgatg cggatggttt
     1561 ttccatcacg gaagtgcagc ttgcagtagg gcatgaaaat caaaaagcgg tgctcggtga
     1621 gcggctcgcg gtcccgcagg taaccggtgt ggtcggagac gacgtggatg tggcgggtgc
     1681 tggtgaggaa ggaggtcagc cagccgatgg ggcctggcct ggggtcttcg gcgctggcct
     1741 gcgcggtgat gccgttgagc gtcggctgca tggagccggt ggggatctgg aagggctggg
     1801 cgatgtagcc ccggatgcca agggcgatga cgatggcgac gaagaagacc tcgatgttct
     1861 cgcccagctc gctatgcggg gcgtgcggca gcgccttctc gcacacgcgg ttgatctcct
     1921 catgcaggga ctcgatgcgc tttttgtcgc gctggcgcat ggccgcctcc aactccgcgc
     1981 gcagcccgca gatttgctcc agcttcgccg gcgccagcag gtcgcgcttg tagtctatga
     2041 agcgggtcac gcccttgtga agctgccggg cgtgcttgat gtaacggggg gtgaggaaaa
     2101 acatgcaggc cgatggtcgg ggtcctgccc gccgcgtcaa gcgcgacatg aggggcggtc
     2161 ttcatgcaaa tggggactgg acaagcgcgc cgcagtgcct ttaaaaaccg cccatgccgc
     2221 aataccacat cgccagggac ggacagcaga tcggagtcca ctcggagcag gacgtcctct
     2281 ctggactggc ctccgggagt tttcgtgaga gcgacctttg ctggactgag ggcatgaacg
     2341 gctgggaaaa gctcggccag cgcctcccgg ccatcggagc ggtgacagtg accgacaatc
     2401 cctacgcgcc gcccgccgcg gtccatctga aagccggccc tccggcggaa tacaagctcg
     2461 cctccctggg gctgcgtttc ggcgcggcca tgctcgacac gctcatcagc atggtcctgc
     2521 tcggcgttcc ctatctattt ttgatcgttc aggcgggagg gttcgaggag caaagtccgg
     2581 accttcagtt cacccccggc gtcattgccg ccatcacggc ccttgtggtt ttcggcctcg
     2641 gtctgctcgg ggtgaatatt tacctgctgg cgacgcgcgg ccagtcgctg gggaagatgt
     2701 ggctgggcat ccgcatcgtc acccatcccg acgcggagcc gccgggtttt gtgaaggcgt
     2761 ttcttctgcg cgcttttgtc aacggtctca tcggtggcgt gccctgcctg ggagcgatct
     2821 acagccttgt ggacatctgc ttcatttttg gcgaggagcg ccgctgcatt cacgacctca
     2881 tcgcgggcac ccaggtcatc gaaggccagc cgccgaaaaa gtgacgctgg cccgctcatg
     2941 cgcctcatcc ggccattcct cagaccctgg ctggcgatgc tcatccggca gcggtcggtg
     3001 tgccgggcgc tgctgctgat gctggctgtg atcggcgccg gctatttgct gggcctgccg
     3061 ctctggccct gcgtgtttgc ggatgtgacc ggcctgccat gcccgggctg cggcatgaca
     3121 cgcgccgcct ccgcgctgct gcggggggaa tgggcggagg cgtggcggtt tcatcctttc
     3181 gcgttcgcct ttgccgtcat cgggttgttc gccgccgccg gggccatgct tccgcgcgca
     3241 tcggccggcg ctctggcggc gaaagtggag gcatgggagg tgaaaagcag gctgacagcg
     3301 atttttttgg cggtgctgct catttttagc ttgatgaggg tcttggggtt ttggtatcag
     3361 cccccgttgt cccgcccatc cctgtttttt ctcattcaag aaatgcaggg tggcggcggt
     3421 cttcgtcatc aaactgaata acacatccac tcaaaactat ggaacccaca tccactgcgg
     3481 cagcagccac cggcgtcgga ttggcctctt tggccgcctt cggcgtcatc ttcatcatca
     3541 acctggtctg ctggatcaag gtgctcatcg ccctgttcaa gaaggagggc gccggcctcg
     3601 gcatcctcgg cattttgtgc agcatctacg cctatatctg gggctggatg aagagcacgg
     3661 agcttggcct gaaaaagacc atggtctggt ggaccatcgc catcctggcc agcatcgtgc
     3721 tcagctttgt cagttcggca gccatggttg gcgtcatggc ctcaaatccc gaattccaaa
     3781 aggccatgca ggaggcccag gaaaaagctc agcagctcca gcaggagcag ccggctccgc
     3841 agcagtagtc tttggcgctt cattcacaaa accgcccggt ggtgtcgcca tcgggcggtt
     3901 ttgtcgtttt gaggagagcc atttcaaggc agcctccagg ctttgcgcag cgcggcgtat
     3961 tcggtctccg gcagcagcac ataatgcggg caggccttcg ggtccaccca gcggacgagt
     4021 ctcacaaggt cgggaagcgc catggtggtg cagccggcgc tgggcttgtc cgggccgcgc
     4081 cggacgtgga aaaaaatggc gcttcccgcg ccgggctgca tccacgggcc ctggttgtgg
     4141 cggatttcca gcatccattt gtatgcggcg tcccccagtc gcatgcgctg cttttcgaac
     4201 cagggcggca cggcgcgcgg gtccacgcgc acatgctggt tgtaaaaggg attggaggga
     4261 tcatccacat aggcgtccca aggccccacc tggatgtaag gccagcgagc gccagagggc
     4321 gggcgctccg cgtaaccgaa caatgagcca agctggaaca cgccggctgg cgcccgccag
     4381 tccttctcga ctttcatctg cgtggagctg cgcggcggag tgaagacacc gcgcccccag
     4441 gccattccag cgcggcccag aagcacctgg acgggctgcg cggaagattt ttgccagggc
     4501 gcgcgggcgg actcgcgctg ccacaattcc agcgccgctt tctgcgaggt ccatgccggg
     4561 gcgcgtgcca cgatgagctg gcgggtgctg gcgggaattt gcgcgttaat tcccggagaa
     4621 atcgaataaa tcgaaattaa cacgaaaaat agccgccgga aaagccgtcc cggctgcccg
     4681 acatggcggt gcgcgcttga cgatgaatgg ctcttgtcat cacatggtat ccacaggaca
     4741 aatttttttt ggggtgtttg catttttcgc attgcctcac gactggcggg taaaatataa
     4801 cgcaaatatc aaccctgtcg ggcaattctc ttggcttcag gttttggggg tttttttcct
     4861 gagcgccatg gcccgacagg gttgttttgt ctccgctccc ggcgtggatg ccgtcttgaa
     4921 cattccgcat cacggcttat tcatcgcgcc tgcatgaagc tattgagtat ttcatttctc
     4981 gcggcgctgg tgctttcctc ctgcgcgacg acctcggagg tgagcctgga ttatgtgccg
     5041 tccacaggcc agatcaggcc gggccgtccg gatttttccg tccgtgattt tggcgaccgc
     5101 cgcggcgtgg gaacgcatga gctgggcacg gtgaggacgc agctcggcac gcctgtcgag
     5161 catgtgcgca cccgcgtgcc cgcgcaccag attgtcacga acgctttcgc ccacggactc
     5221 gacgcgcgcg gcatgctctc ccagccgggc ggcgcgcgct tcatcgtcac cggcgaggtg
     5281 ctcgaccttt actgccagat gctggtgcgt ccatacggct acgccagggt gagggtcatg
     5341 ctgctggatg ccgccacagg ccaggtgctg cacaccagtg tcttcaaagg cgagcgccag
     5401 gggccggctt acatccctgg cagcggctcg ccggtgccct tgctgcgcga cctggtgtcc
     5461 ggcgcgcttc aggacgctgt ggaccgcgcc ttggacgacc cggtgatccg caaccgcgcc
     5521 gagggaagga tccaggactg gcccggccgg agcggctacg agccgggaat gttgtgatca
     5581 aacttgcgga tactgccagg gcgcgcggta ctcgcggctg agcagtctgg aggcttccgc
     5641 gtcgccgatg atccgctgct tctcagcgtc ccactggatg ctgcgacccg ccttccagga
     5701 gagcatgccg agcatgggca ggcagcttga gcggtgcgcg ctctcaatgt cggcgacggg
     5761 cgcgcgcttg ttttcgatgg cgtccagcag gtcggcccag agcagggcga tattatggcc
     5821 gtccggctcc tgaagctggt ggtcgccgtg ctcgacggct tttttggcgt ccgccgggta
     5881 ataggtccag ccgtcccgcc agccgatgtg cagcacgccg ttctcgccgt agaaaaaggc
     5941 gccgatcttg tgcttctcgt tgttgttgtc ggagaacttg cggtgctccc agacggcggt
     6001 gaactgctcg aattcaaaag tggccacctg gtggtcgggc gcgtcggttg tctgctcctg
     6061 gtcgttgagc acgggcgccc ctgcgatggg ccgcccgcct gtgcagaaaa ctttctttgg
     6121 ccccttttca ccgctccacc acagcacctg gtccagccaa tgcacgcccc agtcacccat
     6181 ggtgccgttg gcgtagtcca gaaagtttcg ccagccgccg ggatgcagct tggtgttgaa
     6241 cggccgcacg ggcgccggtc cgcaccacat gtcccagtcc atgtgatccg gcgcgcggct
     6301 gttcggcttc ggcgtctcaa caccgccccg gccagccaca aaaaggcgaa ccatgccgat
     6361 tttgccggct ttccctgacc gtagaaaatt catcgcttcc acatgatgcg ggccgatgcg
     6421 ccggtgcagc cccacctgga ccaccacacc ggcatcgcgc gcggctttca ccatggcgcg
     6481 gctttcattc accgtgtggc ccgtgggctt ctccaccagc acatgcgcgc cagcctggca
     6541 tgcggcgatg gtctgaagcg cgtgccagtg gtccggtgtg gcgatgatga cgatctccgg
     6601 cttcgccccg gcgagcaatt cgcggtagtc cttgtagcct ttcggttcgt cgccgctctc
     6661 ggacttcaca tcctcgatgc tgttctccag gacgttggcg tccacatcgc acaacgcggt
     6721 caccttcacc cgccccgatg ccatcgcctc gcgcagcaga ttcatgcccc accatcccga
     6781 accgatcagg gccacacggt attttttccc tgtcgcaccg atgacgggca ggcgtgacat
     6841 ggcgagtgat gcggcggtgg cggatgtgag gaaggcgcgg cgggaggtca tggcgggaaa
     6901 tgcggagaca ttaaacgaca caacgcaccc gctcctgtcc agcacggatg cctgccgtgg
     6961 tcaaaaggcc gggagatcga actgaaagcg gcggcctcct gctatgcgat caactcaggt
     7021 atcgcccggc cattctccac gatgtgtacc ggacggccat tgaggtcgtt gagggtgact
     7081 ttttggtagt cgatgccgag gtggtggtaa atcgtggcga ggaagtccgc ggcgttgcac
     7141 ggacgttcga ccacgtcttc gccgcgtttg tcgctggcac cgatgacccc gcctgtctgg
     7201 atgccgccgc cggcccacac attggtaaag gcgcgcggcc agtggtcgcg accgggctgc
     7261 agcgtgcccg tggggccgct ggcatcaccc gcgccggtgc tgcggtcgaa gctgatcttc
     7321 ggagtgcggc cgaattcgcc ggtgacgacg acgagcactt ttttgtcgag gccgcgcgca
     7381 taaatgtcct cgatgagcgc ggacacgcag cggtcgtagg tcggcatgcg gaaacgaagc
     7441 gcctcgaact gatgctggtt caccgcgtgg tcgtcccaat tgttcacacg gccgcatagc
     7501 ggaccgctga ggctgctggt gacgatttcg acgccggcct cgacgagacg gcgggcgagt
     7561 agaagttgct ggccccaacg attgcggccg tagcggtcac gcgtggccgg atcttctttg
     7621 ctgaggtcaa aggcatcacg cgtcttcgga ttcgtaagca gcgtggcggc ctgggcttcg
     7681 aattcatcca gagcgtcgag ttcaccttcg cggtcaaacg cacgctccat cttgtcgagg
     7741 ctgcggcgca gcgcgaggcg gtcgctcaga cggcgggttt cggcttcgtc atcgagtccg
     7801 atgttgggca cctggaagtt gggacggttc ggatcatcgc tgacggcgaa aggcgcgtaa
     7861 gcatcgccga gatacgccgg gctggagtat tccggcgagg cagcgggcac gccgatgtaa
     7921 ttcggcagcg gattggcacg accgccttct ttggctctga gataatgcgc cactgacatc
     7981 cagtccggca gcctcggctt cggtttgtcg cgggtgtctt tgtcgccgga aaacatctgc
     8041 atcgtgcccg ctggatgacc gcccgcggat tggttcatcg agcgcacgat ggtgaacttg
     8101 tccgcgatct tcgcgttcat cggcagcagc tcggtgaaga tggtgccggg caccttggtg
     8161 ctgatcgttt tgaaggggcc gcgatactcg ctgctcgcgt ccggcttcgg gtcgtaggtg
     8221 tcgatgtgcg actgtccgcc gggcagccat accatgatga tcgacttgcg ctccgacttc
     8281 ggcttgaggg cattttctgc gcgcagcttc agcaggcccg gccagctcag cgtcgccatg
     8341 ccggaaaggc cgaactgcat gaagctgcgg cgcgtcagcg gacctgcgca acgcacgaga
     8401 gacgggggct ggatgatgga ggacattcgc tcaaacctac gcttgatggc atcagcgttt
     8461 atctcgttcg gtctgcgttg gaccatagat tccggcagta ttcccagtga cgcgaattgt
     8521 cgaagcgttc caggttgtca tgaacagtca caatcctgca cacatctctt gccaccaaat
     8581 cgcatttctt ctccaccagt tcggcaggga tcgaagtcaa gacatgcgac tttagctgtt
     8641 cgatcaatcc ctcgattcga cggagttcct gcttctcttg cgtcgaaagg ccaggtggcc
     8701 gggcttcagg aagcggtatc gcctccacat tgttcaacca gcagcgcatg gcttcaaggt
     8761 gctctgtgag ttcggaatcc atagtgtaat gtgagcgcta aacgagcttc caacccattg
     8821 ccgcattgat cttgttcaca atctggcggc ggcggacttg ggcgacggtg ccgcgctttc
     8881 ccttgagttt ggatttctcg acgagccaga gggcatcgca acgacagaga gtctcccagt
     8941 cgaggccatc cgcgccgttg aggcggactt cggtttcttt gggcggacga ttggcccgct
     9001 gggtggtgca gaggagcacg ttgatccagt cagctttggc gacgcggtcg gggtgcgaaa
     9061 caatcaccgc cggatgcgcg cccgctccct caaactcata ggtgtagatg tcccagggct
     9121 tcatacctca tccggacggg ggatgcgctg tttgcgggcg agtttgcctt cctcgcggct
     9181 gctctgggtg gtgtagaggt gggcaagcga accgggcggg aagctctcgc cctcgctctc
     9241 ctggagtttg cgcagcgctg cacggacgac ctcgcttgcg ttgttgtagc ggcctgaggt
     9301 gacgagctgc ttgatgaagt cttcaaaatg gtgggtgagc gcgacgttca tgccgccaac
     9361 tattgacatt agatgtccag agtcaacccg gaggcttggg ctgattcctt ggccagtccg
     9421 tctgaacaca gactggaaag accctgttac ttcgcaccaa acgaagccag cgccttgccg
     9481 gtgctgtccc agatccgcag caggctgtcc tcgccgccgg cgacgagggt agtgccgttc
     9541 ggcgtgctga cggcggcctg catgtagtcg gggaggtcgg ggatattgcg gatctcgttg
     9601 ccttcgtcgt tcacgatgcg gacgcggttg tcgccggcgg aggtgaagat ctgattcgtc
     9661 gcgccgatga attgcagcga ggtgacctcc ttggcccagc cttcgatctt tcgcttccgc
     9721 tcgccgatga tcatgtccca ggtcaccacg gtgccttccg cgccagcgct ggcgagcacg
     9781 cggccatcgc tgcggaatgc gacggcggtg acgtgatgcg tgtggccctc aagaaggttc
     9841 agctgcctgc ccgtgaagat ctccgtgaca cgcacgttct tgtccaaaat ctggcggcgg
     9901 cggacttggg caacggtgct cgctttccct cgagtttgga tttctcaacg agccagaggc
     9961 catcgcgccg ttgaggcgga cttcggtttc tttgggcgga cgattggccc gttgagtggt
    10021 gcaaagcagc acgttcaccc actcagcttt ggcgacgcgg tctggatgag aaactatcac
    10081 cgccggatgc gcgtccgccc cctcaaaatc ataagtgcag atgacccagg gcttcatacc
    10141 tcatcgggac tggggatgcg cttttgcggg cgagtttgcc ttccgtgcgg atgctctagg
    10201 tgatgtggag gtgggcaagc gaaccgggcg ggaagctttc gcccttgctc tcctggactt
    10261 tgcgcagcgc ggcacggacg acctcgctgg cgttgttgtg gcggcctgag gtgatggaaa
    10321 gcgcctcccc acaaggacgt ctcacctgcc cagcagccgg cggacttgct cctcgctgta
    10381 ggctgggttg cgccggtttt cctcaagctc gatgaagacg ggccggttgt ttttttcctg
    10441 gctgcgcaaa acgtcgcggt tcgcgcttgt gccaaatgtg tccccggcat cgcgaaaggt
    10501 ctggccggcc tggcgtgcgg tcttgcttgc aaggcggctc tcgcccgcgg cgaaagcatc
    10561 atccgactcg ctcgccacct ggtcccgccg ggcgaaggac tgcccgctca tgccgctctg
    10621 cttgtctttg ccggagtagt ccgtggtctt gtagccgggc gtgacgctgt agcttttgga
    10681 gcctttgaaa gcgcccggct tgtaggactg cgaggtgatg cgcgaaccgg agcctttgtt
    10741 gcgcgccagg gtggactgca cgccgggatc aaagcggctg cggtcgttgg gatcagccat
    10801 gcgtttgcgg ctggcggcga accgctgctc catattctgg gacatgagcg gcgtggcctc
    10861 gcgcttcgac gcccggtcct gctctgacga ggagcaggat gccaaaagaa tgaccaatgg
    10921 aagtgtgacg cggtgcattt caaaaatgaa tatcctgctt cgaggggatt cgaaagacaa
    10981 aaccttgcag cctctgccat caccacagcg gcgacttggg cggggcgctg gggaaggagt
    11041 agccggagga gccgtcgaat ttgaaggcgt tccagccgtt gctgccgctg aagatgccca
    11101 ggtcaaccgg ccaggtgccg ctgatccggg tggtgccgtc aatgacgagc ttgccgccag
    11161 tgctcacctt gccgccgagc tttaaagaca tccatttcgt ggggacaaag gcttcgacag
    11221 tcttcttttc cgcgaggctc tggagaaaaa cggtgccacc ggcgtgacca tcccgcgcaa
    11281 aggctaccag aagctcagcg agcaacccca gctcttcgac cgagtagtct atggacaccg
    11341 gcacgaggtc gagaactgct tcatgcacct caaagcccac aaacgtttag ccctcagagc
    11401 agagaagacg gcgtcctcgc tcgctggctt gtcgtctttg cctcatttct ccgatacatc
    11461 catacccact gatttgcaga gacaccgtct aaaccactcc cgccgctgga tttcaatcgc
    11521 tcagaaccgg agaaagcgac tccgttttga ccgcctgcca ggcattgaac aagacttctt
    11581 tttcggtgcc gcgcggcagg tggtatttgg gcagctcggc gcggaggtgc tccaggcggt
    11641 cccaggcctg gtaaagctcg aagatgaggg cggcggagtc ggcgggcagt gtccagtgct
    11701 ggcgcaagag gaagacctcg gcggcgggga agtcccgctc ctcgatgagg aagcgcgccc
    11761 aggcggcgct gagggcggtg tgcagaaggt gggagccgtc gtttcgttcg atggctgcct
    11821 ggtagagttc gcgggcgagg ccgcgctcgc cgatctgggc gaaggcccgc gcccactcca
    11881 cgggctggct gccgccaggg aagcttttgc ggacgacctc ggcaaagagt tcgcgcacgg
    11941 cggtgtggtc gcccatggcg tgggcgacgc gggccatgag ggggcgcttg cggccttcga
    12001 tgcggagggt gggcaggtct ttgagggtct ggcaggcgtg ccaggcccag cgcgggcggc
    12061 ctgagtcgag cagggtttcg gcgccgagtt cgaggcaggt gtggtcgcct tcgcgcgcgg
    12121 cggaccagcc gtcggcgagg tcggcggcgg cggattccgg caggtctttg gcgaaggcgc
    12181 tgagggctag cgcggcgagg gggcggtcaa gcccggcgcg tgcctcggag cggagcaggg
    12241 tggtccaggc gtcgcggttg ggcccatgga actgggcgtt catccagtcg aggaaaaggc
    12301 cgagggtctc gcggtcgcgg ctgcggacgc ggaacatgga ggcgacctgg gagcggccga
    12361 ggtcgggtgt ccaagcgggg tcggcggtgt gcaggcggag ctgctcgaag cgcaggcgga
    12421 agcggtcggc atcgtctttg aggacgcgct cggcctggat gagatagttc agcgcttccg
    12481 tgccgcgctc gtggcggctg agaagcccgg cgaggtggat gatggaggcg agcgtttttt
    12541 tatccacggc cagggccatg agcggacgga aggcctccca gccgcccgac tgcgccagca
    12601 actccgcgcg caggtgcagg atgtccctcc aaaaggggtt caattttccg ccaaggtcca
    12661 cctgttcgag ggtggcgagc gcggccacgg gcagggattg tttttgcagg aggcgggctt
    12721 tgtgcaggca ggcttcggga tcgggaggcg ggctttcctc cccgaggctg gtctgaaaag
    12781 cgaggagcag attttcccag gcattgagcg cctcatcctc gcggcccagg cgctcgcaga
    12841 gcagggccag ctcgcgcaga taaggcacgg agtgggggat gcggccggga aggatgcgcc
    12901 ctgcctgcgg cttgccgccg aggcggcgca gctctttgag gtcgtagtcc tgggccagga
    12961 aatgggcgtg tttttcccgc aggacctcgt ctccgggttg cgggggtttc atgggcggct
    13021 cggcctggag gagctggagg gtgaaggcga ggccggtggg cgtgtcgcgc gcggcctcgc
    13081 tggcgcggat gagccagagg gcgtattcgg ggttggcggg ggcgcgtccg ggcaggagcc
    13141 gatagacttc gacggcctcg cgcgccatgc cgttgctttc caggcggccg ccgagctcgc
    13201 tgagggtgtc cacggagact tccatgcgca tgtccacgct ggcgaggtgc tcgcggatga
    13261 ggcgcaggcg tgtggggtgg tcggagaggc gaaggcggcg catgagctgg ccgagacgcc
    13321 attctccaaa ttcgaagccg ctgcgggagc tgagcgtcat ggcgccgagg cgctcatcaa
    13381 gccgcccgcc ggcctcctgt acgactccgg ccaggccctg ctggacgagc acggcctgga
    13441 tttcgcgcgt ttcctcccag aagctgcgca gatgcatggg ctcctcgtgg cggttggagc
    13501 cctgcggcat gagaatgccg cgcatctcgc ccatctggcg cgcggccagg aagtcgcctg
    13561 tgaccaggtc ctcaagctcg ctggcggcgc ggccctcggc gccggcgagt ccggcgacga
    13621 ccgttttgcg cacgcgggtc atggctgagt cgggcgtgct ctgaagatgc ttccacgcgg
    13681 tgttgagtgt ctcctcccgc tcctggacgg acatggcgag gcggctggcg gtggcgagac
    13741 cgtagatgta ggggtcgtaa gtgccgcgat aagggcccgg ccgcacgggg ccgtggacga
    13801 ctctgccgag atattcgcgc gccagatccc agcgctcggc ggactcggcg atgcgggaga
    13861 gaaagtaggc gtagtcggcg gccaggccgg gcgagttttt ccgcaggctc tcgcccaact
    13921 cgagcgcctc cgtgtaacgc tggcggtcct ggagggagcg ggtgatctcc agcacggcgg
    13981 agtttggcag caggcgggag gcgccgagag tggcgaggtt gccgctggaa taacgaaacg
    14041 cattgtccgc cgcgagcatg gccgcgcagg catggagcag gcggcggcgc gcctcctgcg
    14101 cggtgatgtc cagcccggtc tgcccggccc aggccagcgc gtcctccatg ccgtgggagc
    14161 gcagcagcag ccagaccaga gcgaagcgcg cctccaacga gggtggagcg ccgagcacct
    14221 ggacgagcag cgcgcggaac tcggggcccg cgccgcaata ataaagcgcc cacagtctct
    14281 cgatggcggg cagcgcggcg gctgaccatt ctttcaccca tgcgtccaga gccgccgtgt
    14341 cgccgccgcg tttgcgcagc cgcgccagct cctcgatggc gcgcaggcgg atgttttcca
    14401 cctcgtcggg caggacggcg aattccggac gcgggaggtt caaaaagagg cgcagcctgg
    14461 aggtctcctc atgggtgatg ccaggagcgg agccggcgag gtcagccaca tccgaggcgg
    14521 ggccgggcgc gccatccttg cgcaggtcag agacgggcag gggcgtgcgt tctggatgca
    14581 gcgcgcgcgg gctgtgggac tggccggcgg ttttgtccaa aatctcgcgc aactgcgccc
    14641 cggcggcggt gtcatcggcg aggcgcagcg atttcaaagc gatgcggaag agcgagcgcg
    14701 cgtcccaggg gcgcacccgg gcgatctgct catagacgcg gcgctcggcc tcgtcctggc
    14761 cggtggcgcg gtaatgcccg gcgaggcttt ccagagccgc cgcgtcctgg gagaagcggc
    14821 tctccaggcg ccggcgcacg cggtcggcct cggcgatctg gaaggtctgc tcgagcatct
    14881 cgacgaggcg gcgcgactcg ccggccagtt cggcgggcgg ggcggtggcg gcgatttgtt
    14941 tttggaaata gatggcggag gccatgtcct gcacgcgcag cgcggcatcg cgaagctggg
    15001 cggcggagaa aggcgtgtcc ggcgcggtgt agtaggcctg tttcatggcc ttgaaggcga
    15061 gatcgtggcc gccctggcgc tcatggacga gggccagggc ctcgtggtaa aagccgtcga
    15121 acggatgctt gcgcgccagg gtggcgaggt tcttttccag gagttcgagg cgctttttca
    15181 tttcctcgac cggcagttcg ccgccgttct cggtccagag caggcggtcg aggaagcgcc
    15241 ggtaaatttc ccggcggcgc ttcacgccgg gttccttttc caggatggcg gtctggactt
    15301 cgatgtactc ctgctgcctg tgctggcgca tgagcagctc ggcaaagcgc tccatgagcg
    15361 ggatggcgga gccgtccccg gcgcggcgga tggcttcctt ccacagggcg gtcgcatcgt
    15421 tgtgacggcg ctggagagtg taacactggg acagagcgga gaggatctgc tccgagtccg
    15481 ggtggcgccg ccatgcatcc tccaggaagc gggtggcctc ctgcgcgggc ggcgggaggg
    15541 agccgtggag ctgccagcct ttgtggttca tggccagagt tgccaggcgc tcctgctcgg
    15601 ctgccatcat cagctcgctg agacgcagga catggcgtgt gcggccgggc tcgtcccgct
    15661 cgatgaggcg gcgcagcacg cgcatggcga gcaggcggtt ttgatcgctc aatgccagct
    15721 ccagcagcag cttttccgcc tccagggaaa gcgctcgcgg ctgtcctgtg gcggcgtctt
    15781 tttgcaggcg gttcagaaac gggtaggcgg catcccgctg ggcggtgcgc aatgcccacc
    15841 atgcagcgcg gaagagggtg gcgtcactgg cgtcgggcgc ttccgccgcc gccaggcagg
    15901 cccgtgtccg ggtcaccacg gcctcgggca gggggtcctt ttcctggggg ctggagccgc
    15961 cgctggcaaa gcccttgccg gtgaaggcat cgggcagttg aaactcgccc gccgtgccgg
    16021 cgcgcgcggg cgcctcggtc ttccggccgc cgagcaggat ggagaacagg cgctcgtcca
    16081 cctccacccg ctcctcgtcg gtggcgctgg cggcccaggc tttttccagc cattccgcgg
    16141 actcctccgc ttccgcctgc tcggcgagca gatcggccag gcgcaaccac tgctcccgcc
    16201 ccgcgtccgg acgcgccacc agctcgcgcg cgaggatgat ggcgctgtcg aggtcctggc
    16261 cgccggcgag gaagccggac acgtcgttga gcctgcggcc gcgctcggcc tcattggcgg
    16321 cgcctgcggc gaacccgcgc agcagcttgt ccgcggcggc ggtgtttttg cgcgcgcggt
    16381 ggaaggctgc cagttcgaag accgcctcct ggcgcgaggg gtcgcgctcc acgcggcggc
    16441 gcaggatttc ctcaatgacg gtgtccagcg agcgctcctg cgcgaaggcc agcgcctgct
    16501 tttcgaggtc cgtgtccgcg cccgccgcca gcagcttttg cagccgcgcg ccggcggctg
    16561 ccgcgtcacc gaggcgcaga tcagcctcgc agcgcaggaa gacgagggcg ggcgcctggc
    16621 tgtccccgct catggacttg tcgagcaatt cggcggcctc gcgcgcgcca tcgtggtcga
    16681 gcagggcgcg cacgagctgc cagcggtaat ccatgacttg cggcaccgcc ttcaccagcg
    16741 cgcgcagtgc ggccacatgc gcgtcctggt ccacggtgaa ctcgtaaaag cgggccagat
    16801 ccagcaacga gcgctcctca ggcggccggg atcccgcggc atcttccaga cgggtccgca
    16861 tttcatccag aagacccgcg cgctcgtgca ggcgcaccag ccggcggaag aactccacat
    16921 agcgcccgtc gcggaagtct agaaccgtca gcgcctgccg cagcgccgcg ccggctttgt
    16981 caaaatcatc ccccagctcg tggatgcggg ccaggtcgtg gagcgcgtcc aggcggcgcg
    17041 cgggatcact ctgcgcggcc agtttttcat aaaaaaccgc cgcctcagcg acgaggcccg
    17101 cgcgcagcag cagctccgcc acctggcgcg cgagggtgaa gtcccctggg cgcagtgagg
    17161 ccgctttctc ccaggcc
//
LOCUS       DAOMYJ010000098        17168 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Prosthecobacter sp. isolate AMR_MDS_5629
            NODE_1145_length_17168_cov_20.424999, whole genome shotgun
            sequence.
ACCESSION   DAOMYJ010000098 DAOMYJ010000000
VERSION     DAOMYJ010000098.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867850
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prosthecobacter sp. (wastewater metagenome)
  ORGANISM  Prosthecobacter sp.
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Verrucomicrobiaceae; Prosthecobacter.
REFERENCE   1  (bases 1 to 17168)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17168)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.30
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:34:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,742
            CDSs (total)                      :: 4,684
            Genes (coding)                    :: 4,676
            CDSs (with protein)               :: 4,676
            Genes (RNA)                       :: 58
            tRNAs                             :: 55
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17168
                     /organism="Prosthecobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1145_length_17168_cov_20.424999"
                     /isolate="AMR_MDS_5629"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1965333"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            274..1230
                     /locus_tag="PLP58_11570"
     CDS             274..1230
                     /locus_tag="PLP58_11570"
                     /inference="COORDINATES: protein motif:HMM:NF013530.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-binding protein"
                     /protein_id="HRJ09203.1"
                     /translation="MFIVGGTGLLGYHAAQEFLAKGWSVTTLGLPPAPPAALYPPSVA
                     VVLKDVAKATDAELRQLLRGHEGLVFAAGLDDRHLLAKPAYDKFYQANVAALKRLLSC
                     AQEAGVRRAVVLGSYFVYFHRLWPALKLAERHPYIRSRVEQEQVATSMVGLEGMVLEL
                     PYIFGAMPVPGWKPLWAPLIKYLRLAPILFYPAGGTACISATVAGRAIVAALERGEAG
                     RCYPVGQENLSWREMLTRLAWAEQRQVQVVTVPTALVTAGLLGVKLFHHLQGKEGGLN
                     LRYMAQLQTAQTFIDPEIACQALGIELDDLDDAFRKTVAACQ"
     gene            1464..1757
                     /gene="gatC"
                     /locus_tag="PLP58_11575"
     CDS             1464..1757
                     /gene="gatC"
                     /locus_tag="PLP58_11575"
                     /inference="COORDINATES: protein motif:HMM:TIGR00135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HRJ09204.1"
                     /translation="MHLSTADVEHIAELARLALTPDEIEQYQRQLSGILEHFETLKQV
                     DTSSVPPTASVLPLRTVMRADQVRPSLPTEDALANAPDREDGFFRVRAVFSEE"
     gene            1925..3379
                     /gene="gatA"
                     /locus_tag="PLP58_11580"
     CDS             1925..3379
                     /gene="gatA"
                     /locus_tag="PLP58_11580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007417080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatA"
                     /protein_id="HRJ09205.1"
                     /translation="MSDELFRLTIHQAATLLRQREISAVELTQAVLERIVAVDNAVKA
                     YLSLMPEAALESAAEADRRLAAADDPASLPPLVGIPLAIKDVILVEGAPTTCGSKMLE
                     DFVAPYEATVIGKLRRAGAVLLGKTNTDEFAMGSSTENSAFVTTRNPWDLSRVPGGSS
                     GGSAAAVAGDLAIAALGSDTGGSIRQPAALCGCVGLKPTYGRVSRYGLVAFASSLDQI
                     GPLTRTVEDAALLLNHLCGKDARDSTSLDAEAPDFTAALGRDIKGLRIGLPKEYFIEG
                     IHAGVSASVKAAVERLAALGAELVEVSLPHTDLGVATYYILAPAEASANLARFDGVRY
                     GHRSSEAGDLMEHYFKSRAEGFGPEVKRRILLGTYVLSSGYYDAYYLKAQRARTLIRR
                     DFEQAFEKVDVLVSPTSPAPAHKIGELKDNPLAAYLEDVFTIPVNLAGLPGISVPCAP
                     VEVEGVSLPVGLQIIGKPLDELSVLRVADAFERG"
     gene            complement(3416..4252)
                     /gene="accD"
                     /locus_tag="PLP58_11585"
     CDS             complement(3416..4252)
                     /gene="accD"
                     /locus_tag="PLP58_11585"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018970438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase, carboxyltransferase
                     subunit beta"
                     /protein_id="HRJ09206.1"
                     /translation="MGFFKKRTVSDLGEKKKDMPEGLWTKCPSCGESLFEQALLKNLR
                     VCTHCGYHFTISSGERIQSLVDEGSFSEMDLQLDSVDALGFKGYLEKVKAYQNKTGLK
                     EAVVTGRASIETIPVLLAIMDFRFLGASMGSVVGEKITRAIEAATAEGKAVIVFSSSG
                     GARMHEGILSLMQMAKTSGALARHAEARLPYISVLTHPTTGGVTASFATLGDIIIAEP
                     KCMIGFAGPRVVKETTHADLPPGFQTAEFMMKHGLVDMIVERKDMRATLGGLLRYVSP
                     RQ"
     gene            complement(4385..5158)
                     /locus_tag="PLP58_11590"
     CDS             complement(4385..5158)
                     /locus_tag="PLP58_11590"
                     /inference="COORDINATES: protein motif:HMM:NF039185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HRJ09207.1"
                     /translation="MRHFLHKAGDSLLDLLYPRGCFACDALLGMDAPAAGDGAWLCSG
                     CLETVVKIEAPYCSVCGEAFDGALTAPFRCWNCEERKLDFDFALSACKAGGAVRDMIH
                     AFKYHRNLAMRAPLADMMLPVFQDPRLAAEDLSQWLLMPVPLHPLRWIWRGYNQSAEL
                     CRMLSRHTGMTVAHALARIRLTRPQAGLERRSRLKNLRGIFRLRRFPRLMRGRDLKGK
                     NIFLVDDVLTTGATTHECARVLKRQAGVEKVVVITAARG"
     gene            5277..5828
                     /locus_tag="PLP58_11595"
     CDS             5277..5828
                     /locus_tag="PLP58_11595"
                     /inference="COORDINATES: protein motif:HMM:NF012257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030551 - cyclic nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HRJ09208.1"
                     /translation="MLEIKRLTVPAYGFAKNLDPERRQLLAICGQFFTFEEGEVIVNE
                     GDTHTDLYFVLEGTLMLRRRINEHSYAPIGTAKRGSTFGELNLFHPLPARTTILAVTS
                     GEIWRLSKEQFEAMIADDLVIAREALGYLCTTLALRLRRATERYANTKEEYDQLFDDL
                     VEERSLDEVLPGVEPPPEQPAAE"
     gene            complement(5850..7673)
                     /locus_tag="PLP58_11600"
     CDS             complement(5850..7673)
                     /locus_tag="PLP58_11600"
                     /inference="COORDINATES: protein motif:HMM:NF015083.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxidase family protein"
                     /protein_id="HRJ09209.1"
                     /translation="MKTNIHPFGKTILLAAAVFALGIHTATAQTRQLRPPPPRPSANS
                     TPAAPIPRADPGRPEPPRPPQTPPPRQNPPPPKTRQPLPPALPESFVMPEQFRSHDGR
                     SNHATDTERGAAGRIFIRLAPGDYADGAGEPAGAQRPSPRAISNAVSAQPAPRVNGRG
                     ASDMLWQWGQFLDHDLDETPAASPSEPFAIQVPAGDPQFDPAATGAATIGLNRSAYEV
                     VNGKREQKNAITPWIDASQVYGSDEARALALRSNDGSGRLKTSSSAHGDLLPTHSDEL
                     AALPPVPGFFVAGDVRVNEQVGLIAIHTLFVREHNFWADLYRTANPDAGEEEIYQFAR
                     LIVGSEVQAITYREFLPIILGPGALRPYRGYNPDTDPAISNEFATAAYRLGHSLLSPA
                     LLRLDAWKKEIAAGHLSLAGAFFQPGHVTSEGVDVVLRGLCLQRCQELDAWIIEDVRN
                     FLFGAPGAGGLDLASLNLQRGRDHGLPAFAAMRDAFGMRPVKTFKDISPFGATAAQLA
                     SVYAQPGDVDLWIGGLAEAKKPGAMVGPVFHRILADQFTRLRDADRFWYQNIFSKEMI
                     RMIERQTLSVILRRNTGLGREIPDKAFLAPPPPARSAAPRR"
     gene            complement(7849..8700)
                     /locus_tag="PLP58_11605"
     CDS             complement(7849..8700)
                     /locus_tag="PLP58_11605"
                     /inference="COORDINATES: protein motif:HMM:NF025600.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YHYH protein"
                     /protein_id="HRJ09210.1"
                     /translation="MPATEKPPGENEVRILRRGPSLDITSNALPDHLVGRFPNRGNPH
                     HIEAQRYDFRLPADPQPAAQTTWLHTQPAGERGPPNMPFGIALNGVLFDPGTAEFWQG
                     DRALGWNYEALGGAVPLGLDASHAHVQPGGVYHYHGLPTLLMDQLGLAADQHSPLIGW
                     AADGFPIYALRGYKDPQDPASGMKTLRSSYDLKPGERPRPPKGPGGPHDGAFVQDYVF
                     IAGHGDLDECNGRFCVTPDFPQGVYAYFLTAEWPVIPRAFRGTPVVLRGAGGPGGPGR
                     GKGSRPR"
     gene            8938..9915
                     /locus_tag="PLP58_11610"
     CDS             8938..9915
                     /locus_tag="PLP58_11610"
                     /inference="COORDINATES: protein motif:HMM:NF013292.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoaspartyl peptidase/L-asparaginase"
                     /protein_id="HRJ09211.1"
                     /translation="MKAWLFPLLLCCGFVSADESPITLVIHGGAGVGRESLTPDKEKQ
                     CRQVLEQAVREGHAVLVKDGAALEAVQTAVMILEDSPLFNAGRGAVLTAAGTAELDAS
                     VMEGRTGQAGAVAGVQGVRHPIQLAEMVMRKTPHVLMIGRGAEELARAQGLRFEDLDW
                     FITPEQVARLERARKKSAALDDNDFSLRMGTVGAVALDRQGNLAAGTSTGGLVNKRSG
                     RVGDSPVIGAGTYAENGVCAVSCTGHGEFFIRNVVAYDVAARMKYGGASLADAAEAVI
                     NEKLKKLDGRGGLISVDARGGIAMPFNSDGMFRAWIGPDNVPHVAIFQD"
     gene            complement(9980..10056)
                     /locus_tag="PLP58_11615"
     tRNA            complement(9980..10056)
                     /locus_tag="PLP58_11615"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(10020..10022),aa:Met,seq:cat)
     gene            complement(10211..13183)
                     /gene="ppdK"
                     /locus_tag="PLP58_11620"
     CDS             complement(10211..13183)
                     /gene="ppdK"
                     /locus_tag="PLP58_11620"
                     /EC_number="2.7.9.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009962814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050242 - pyruvate, phosphate dikinase
                     activity [Evidence IEA];
                     GO_process: GO:0015976 - carbon utilization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate, phosphate dikinase"
                     /protein_id="HRJ09212.1"
                     /translation="MAKKASNSSSKAVKYVYSFGAGKADGDGSMKALLGGKGANLAEM
                     SRIGLPVPPGFTITTEVCTFYYANKRSYPADLQKQMEEGIAKMEKIMACKFGDAKGMP
                     LLVAVRSGARDSMPGMMDTILNLGLNDQTVLSLASATNNERFAWDCYRRFVQMYGDVV
                     LGVQKREGEDHEPFETVIEGYKHEVYGKDIVDSDLTAADQQELVKRFKALVKERTGKG
                     FPDSPWDQLRGAAGAVFGSWMNDRAIVYRRKYNIPAEWGTAVNVQAMVYGNTGNDSGS
                     GVAFTRNPANGINEFYGEFLINAQGEDVVAGVRTPEPVAQLQAEMPKAYAELMKVRAI
                     LEKHFKDVQDVEFTIQQGKLFMLQTRNGKRTAAAALKFSMDMVKERLIDWETAILRNP
                     AEQLDQLLAPIFDHEDVRNAKALATGLPAGPGAASGKIYLNADRAAAAEQRGEKVLLV
                     RNETSPEDLRGMIAAEGILTAKGGVSSHAALVARQMGKVCICGASALEINYDKKTVTV
                     ASKTFKEGDFLSIDGTSGTVYGGQLKTAPSEIITGMLHGDKAAQGTEKFQHFNQLMKW
                     CGKVTRLQVRTNADNPEQTQNAIAFGAQGIGLTRTEHMFFEGDRIDAMREMILADNEK
                     DRRAALAKLLPYQREDFTGIFESLKGLPATIRLLDPPLHEFLPHTAKQQKELASKMGI
                     SADKIAHRVEQLHEFNPMLGHRGCRLGIAYPEITEMQARAIFEAAADVAKMKIKVKPE
                     VMIPLVGFKKELDLQTEIVLRVAKEVMAEKKVKIDYMVGTMIEVPRGALTADEIAQTA
                     EFFSFGTNDLTQTALGISRDDMGSFLMPYTENEIFKKNPFATLDQTGVGQLIKIAIEK
                     GRSTNPGIKLGICGEHGGDPDSVKFCHKVGLTYVSCSPFRVPVARLAAAQAAIEEKRA
                     AEKAANGKDVRGGSSAASSAKQNNKATNNKRNNNMAKKAAKKAPAKKAAPAKKAAPKK
                     APAKKAAPAKKAPAKKGK"
     gene            13635..14273
                     /locus_tag="PLP58_11625"
     CDS             13635..14273
                     /locus_tag="PLP58_11625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007415225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HRJ09213.1"
                     /translation="MKTFAKFQHTTRRGFTLIELLVVIVIIAILASLAIPVGNLVMDR
                     TNRLRVQKTMKDVIVAVNHYRTEYNRFPIGINDATGGDSDMAPFLTDGNGTPIINILM
                     ANTQDGNEDMNPRKIKFVDIPFARNNQFGIISQSGGANDGAPVQLVDVWGQPYSIALD
                     TNYDNLIENPDKQNNDLTISSRATDNVSASAIMWSYGKDRQQGTKDDIASWR"
     gene            complement(14359..14859)
                     /locus_tag="PLP58_11630"
     CDS             complement(14359..14859)
                     /locus_tag="PLP58_11630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009964445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase subunit E"
                     /protein_id="HRJ09214.1"
                     /translation="MAFAVPAELESKMDEVITHYPVSKRSAVLPLLHLMQEHFRFISE
                     DIVAWVAAKLGLEPIQVLEVVTFYPGFRQTAPGKYHIRVCRTLSCAMAGSFDLMEALC
                     KEAGIDRSHCDHHHPIAVSADGKFSIEFAECLASCGFGPVCMVENDFHEKVEPAKASV
                     LLARYS"
     gene            complement(14896..16155)
                     /gene="nuoD"
                     /locus_tag="PLP58_11635"
     CDS             complement(14896..16155)
                     /gene="nuoD"
                     /locus_tag="PLP58_11635"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009964446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH dehydrogenase (quinone) subunit D"
                     /protein_id="HRJ09215.1"
                     /translation="MPTVTREHEAPDTAAKAARHLESMEETAEDIVGEKLVLNMGPSH
                     PATHGVLRLVLEMDGEVITKADPDVGFLHRGDEKIAENMHYNQFVPYTDRLDYLAPLA
                     NNVAYALAVEKLMGWEVPERGQAIRVICCELARISAHLLGVGVFAMDVGAMTAFLYTF
                     TEREKIYNLCEQLTGARFTTSYTRVGGQIRDLPEGFTAAVKKFLDEVLPVIDEIDKLL
                     TRNAIFINRTQGLGVISKEDAISFGLSGPNLRGSGVDHDLRKTHPYLHYDRYEFDIPV
                     GSTGDCYDRYLVRMEEMRQSARILRQVLASLPGGPINVADAKNLLPQKERVLMKMEEL
                     IHHFIIATQGIDAPAGEVYFGAENPKGELGFYIHSKGGGVPYRLKIRSPSFVNLSILP
                     KMLKGHMLSDIPAVLGSLDFVMGECDR"
     gene            16380..>17168
                     /locus_tag="PLP58_11640"
     CDS             16380..>17168
                     /locus_tag="PLP58_11640"
                     /inference="COORDINATES: protein motif:HMM:NF040105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HRJ09216.1"
                     /translation="MSARLDASESPVVTTAQAREAADRLGVLVAGLSEIILGKEDVLR
                     LAVTCLLARGHLLFEDLPGVGKTLLSQALAQALGLRFRRVQFTSDLLPADILGANMYD
                     RQSGGFVFHPGPVFAQVLLADEINRATPKTQSALLEAMEEGRVTCDGESHALPAPFFV
                     IATQNPSTQMGTFMLPESQLDRFLMRLALGVPDRAAERSILQGRDRREALREMPELLS
                     WEAMSAMQQQARHVHVSPALLDYLQDLLAATRAEGRGLSPRGGLA"
BASE COUNT         3125 a         5088 c         5636 g         3319 t
ORIGIN      
        1 tcaacaccga gcagatgaac caggccgtct tcgccttccg gcgctacgaa gacgcatcgc
       61 tgcgctacac cggcatcgac agccacccgg ggctgaccca ctacgggctg tatgacacgg
      121 tggtggccag ggagtgacgc gcggatcgca aatcctgcca aaagacttgg gtgttttcaa
      181 aacgggttga acacacccaa gccaattcgc aggaagcttg ccgaacactg aatgcaatgc
      241 aggtgtccag aggaaaagtc tatgtcgaaa cgagtgttca tcgtcggtgg aacagggctc
      301 ctgggctacc atgccgccca ggaattcctg gcaaaaggct ggagcgttac cacacttggc
      361 ttgccgcccg cgccgccagc cgccttgtat ccaccctcgg tcgccgtagt gctcaaagac
      421 gtggccaaag ccaccgatgc cgaactccgt cagttgctgc gagggcacga aggcttggtc
      481 tttgcggctg ggctggatga tcgacatctc ctggcgaagc cagcctatga caaattctac
      541 caggcgaatg ttgcggccct caagcgattg ctctcctgtg cgcaagaggc gggcgtccgg
      601 cgggccgtgg tgctgggttc ctattttgtt tacttccatc gcttatggcc ggcattgaaa
      661 ctcgctgagc ggcaccccta catccgatcg cgggtggagc aggagcaggt agcgacttct
      721 atggtgggtc tggaagggat ggtgcttgaa ctgccgtaca tttttggggc catgcctgtt
      781 cctggctgga aaccgctttg ggcgccgctg atcaagtatt tgcgtttagc ccccatcctt
      841 ttctatcccg caggcggtac ggcctgcata tcggcgacag tcgcaggacg tgcgattgtc
      901 gccgctcttg agcgaggcga ggcaggccgc tgctatcccg tcggtcagga gaatctgagc
      961 tggcgggaga tgctcacgcg attggcctgg gcggagcagc gccaggtgca ggtggtgacc
     1021 gtgcccaccg cgcttgtgac cgcagggttg ctgggcgtca agctttttca tcacctgcaa
     1081 ggcaaagaag gagggctgaa tctgcgctat atggctcaat tgcagacggc gcagaccttt
     1141 atcgatcctg aaatcgcctg ccaggcgctg gggatcgaac tggacgatct ggatgacgcc
     1201 tttcggaaga ccgtggccgc ttgtcaatga agcggctgaa tgtgccgctg aaacctcctt
     1261 gacagcttgt ccaatacccg ttgtaccact taccactatt caaccacatg gccctgcgat
     1321 gtcaagcaca aatggcgcca acgcacgaat cgcctgattg ccgcaatccc cccatcctat
     1381 ggtaaaatgc catcggtcgt catacggccg cacaaacaaa caaacgcacc atcgcacaaa
     1441 cgaactaccg aacaagacca cctatgcacc tatccaccgc cgacgtcgaa cacatcgccg
     1501 agctggccag gctggccttg acgcctgacg agatcgagca gtaccagcgc cagctttccg
     1561 gcatcctgga gcacttcgag accctcaagc aggtcgatac ctccagcgtg ccgcccacgg
     1621 ccagcgtcct gcccctgcgc accgtcatgc gcgccgacca ggtgcgcccc agcctgccca
     1681 cggaggacgc cctggccaac gcgcccgacc gggaagacgg cttcttccgc gtgcgcgcgg
     1741 tgtttagtga ggagtagcag gtggcaggtg gcacgcatca cgcctctcgt ttcaccggat
     1801 acaaccgacg gctcttcaca cccctgcgca acgcaaaggc cttcccatcc tcgcacattc
     1861 cgggttacga gttacgcatt gcgcattaag tttcaccgaa ttcccccatg aggagaacac
     1921 accggtgtcc gacgaactgt ttcgactgac catccaccag gcggcaacgc tgctgcggca
     1981 gcgcgagatc tcggctgtgg agctgacgca ggcggtgctg gagcgcatcg tcgccgtgga
     2041 caacgcggtc aaggcttatc tcagcctgat gccagaggcg gcgctggaga gcgcggccga
     2101 ggcggatcgc aggctggcgg ccgcggacga cccggccagc ctgccgccgc tggtgggtat
     2161 cccgctggcc atcaaggacg tgatcctggt ggagggcgcg ccgaccacct gcggctcgaa
     2221 aatgctggaa gattttgtgg cgccctacga ggccacggtg atcggcaagc tgcgaagggc
     2281 cggcgcggtg ctgctgggca agaccaacac cgacgagttc gccatgggat cctccaccga
     2341 gaactcggcc tttgtgacga cgcgcaaccc gtgggatctg agccgcgtgc cgggcggctc
     2401 cagcggcggc agcgcggcgg ctgtggcggg ggacctcgcc atcgccgcgc tcggctcgga
     2461 cacgggcggt tcgatccgcc agccggccgc gctttgcggc tgcgtgggct tgaagccgac
     2521 ctatggccgc gtgtcccgct acgggctggt ggcctttgcc tcctcgctcg accagatcgg
     2581 cccactgacc aggaccgtgg aagacgccgc gctgctgctg aaccacctct gcggcaagga
     2641 cgcgcgggat tccacctcgc tcgatgctga agcgcccgac ttcaccgccg cgctggggcg
     2701 cgacatcaag ggcctgcgca tcggactgcc caaagaatac tttatcgagg ggattcacgc
     2761 cggtgtctcc gccagtgtga aagccgccgt ggagcggctg gcggccctgg gcgcggagct
     2821 ggtggaggtc agcctgccgc acactgactt gggcgtggcc acttattaca tcctggctcc
     2881 cgccgaggcc tcggcgaatc tggcgcgctt cgacggcgtg cgctacggcc accgcagcag
     2941 cgaggcgggc gacctcatgg agcactattt caagagccgg gccgagggct tcggccccga
     3001 ggtgaagcgg cgcatcctgc tcggcaccta tgtgctcagc agcggctact acgacgccta
     3061 ttacctgaag gcccagcgcg cgcgcacgct catccggcgg gactttgagc aggcctttga
     3121 aaaagtggac gtgctcgtca gccccaccag tccggctccg gcgcacaaga tcggcgagtt
     3181 gaaggacaac ccactggctg cctacctgga ggacgtgttc accatccccg tgaacctggc
     3241 cggtctgccc ggcatcagcg tgccgtgcgc gccggtggag gtggaggggg tgtcgctgcc
     3301 cgtggggctg caaatcatcg gcaaaccact ggatgagcta agcgtgctgc gcgtggcgga
     3361 tgcctttgag cgcggttgaa agtggcgcaa cccttccagg ttgcatggca tcacctcact
     3421 gccgcggact gacataacgc agcagcccgc ccagagtggc gcgcatgtct ttgcgctcga
     3481 cgatcatgtc aaccaggccg tgcttcatca tgaactcggc ggtttgaaat ccgggcggca
     3541 gatcggcgtg ggtggtctcc ttcaccacgc gtggtccggc gaagccgatc atgcattttg
     3601 gctcggcgat gatgatgtca cccagcgtgg cgaagctggc ggtgacgccg ccggtggtag
     3661 ggtgcgtgag cacggagatg tagggcaggc gcgcctcggc atggcgggcc agcgcgccac
     3721 tggtcttcgc catctgcatg aggctgagga tgccctcgtg catgcgcgcg ccgccggatg
     3781 aggaaaaaac gatgaccgcc ttgccctcgg cggtggcggc ctcaatggcg cgggtgattt
     3841 tttcccccac cacgcttccc atgctggcgc cgaggaagcg gaagtccatg atggcgagca
     3901 gcacggggat ggtctcgatg gaggcgcgcc cggtgacgac ggcttccttg aggccggtct
     3961 tgttctggta ggccttcacc ttctccaggt agcctttgaa gccgagggcg tccacggagt
     4021 cgagctgcag gtccatctcg gagaagctgc cctcgtccac gaggctctgg atgcgctcgc
     4081 cggagctgat ggtgaagtgg tagccgcagt gggtgcagac gcggaggttt ttcagcagtg
     4141 cctgctcgaa gagactctcg ccgcaggagg gacacttcgt ccacaagcct tcgggcatgt
     4201 cttttttctt ctccccaagg tcgctgacgg tgcgtttttt gaaaaatccc atgatgtctg
     4261 tgtggtggtg gaatccggct tgcgcgggac cgtccgccct ccccgcatgg caaggcgcgg
     4321 gagccgccgg gaacgggtcc gcgacgctgg ctgacgaccc ggatagaccg gaccggtggc
     4381 gattctaacc acgcgcggcg gttatcacca caactttttc aaccccggcc tgccgcttta
     4441 gcaccctggc gcattcgtgg gtggtggcgc cggtggtaag aacgtcgtcc acgaggaaga
     4501 tgtttttgcc cttcaaatca cggccgcgca tcaaccgggg gaaccgccgc aacctgaaaa
     4561 tgccgcgcag atttttcagc cgcgatctgc gctccagtcc ggcttgcggg cgggtcaggc
     4621 gtatgcgggc cagtgcgtgg gccacggtca tgccggtgtg ccgggagagc atgcggcaaa
     4681 gctccgcgct ttgattgtag ccgcgccata tccagcgcaa aggatgcaga ggcacgggca
     4741 tgagcagcca ctgcgaaagg tcttcagcgg cgagcctggg gtcttgaaac acaggcagca
     4801 tcatgtcggc cagaggcgcg cgcatggcga gattgcggtg gtatttgaag gcgtggatca
     4861 tatcgcgcac ggcgccgcca gccttgcagg cggacagggc gaagtcgaaa tcgagcttcc
     4921 gttcctcgca gttccagcag cggaagggag cggtcagcgc cccgtcaaaa gcctcgccgc
     4981 agacggaaca gtaaggggct tcgattttga ccacggtctc caggcacccg gagcagagcc
     5041 aggcgccgtc acctgcagcc ggtgcatcca tgccgagcag ggcgtcacac gcgaaacatc
     5101 cgcgcgggta aagcaggtcg aggagcgagt cgccagcctt gtggaggaag tgcctcattc
     5161 atgcctttta cgcgggcggg gctccctatg gcaagcggtg tgttttgaaa tgccaatcca
     5221 atctggagtg aaaaaattgg ctgacggcgt ggtgctccat ctgttagcct gtacccatgc
     5281 ttgagatcaa acgcctcacc gtgccggcat acggcttcgc caaaaacctc gacccagaac
     5341 gcaggcagct cctggccatc tgcgggcagt tttttacttt tgaggaaggg gaggtcatcg
     5401 tcaacgaggg tgacactcac acggacctct attttgtgct ggaaggcacg ctgatgcttc
     5461 gccgccggat caacgagcac tcttacgcgc cgatcggcac tgccaagcgc ggcagcacct
     5521 tcggtgagtt gaatctcttc cacccactgc ctgcgcggac gacgatcctg gcggtgacat
     5581 ccggggaaat ctggcggctg agcaaggagc agttcgaggc gatgatcgcc gacgatcttg
     5641 tcatcgcccg cgaggcgctg ggctacctct gcaccacgct ggccctgcgt ctgcgccggg
     5701 ccacggagcg ctatgcgaac accaaggagg agtatgacca gctttttgac gatctcgtcg
     5761 aggagcgctc cttggacgag gtgctgccgg gtgtcgaacc gccgccggag caaccggcgg
     5821 cggagtgagt caaacgcggc gcggacgggt catcggcgcg gcgcggcgct tcgcgccgga
     5881 ggtggcgggg caagaaaggc tttgtcggga atctcgcgac ccagtccggt gttccgccgc
     5941 aggatgacac tgagagtctg gcgttcgatc atgcggatca tctctttgga aaagatgttt
     6001 tggtaccaaa agcgatccgc gtcacgcagg cgggtgaact ggtccgcgag gatgcggtgg
     6061 aaaacggggc cgaccatggc gccgggcttt ttggcctcgg ccagaccgcc tatccatagg
     6121 tcaacgtcgc caggctgggc atagacagag gcgagctgcg cggcggtggc tccgaaaggg
     6181 ctgatgtcct tgaacgtctt caccgggcgc atgccgaacg cgtcgcgcat ggctgcaaag
     6241 gcgggcaggc catggtcgcg cccgcgctgg agattgagcg aggcgagatc gagaccgccc
     6301 gcgccgggcg cgccgaacag gaagttgcgc acatcttcaa tgatccacgc gtccagctcc
     6361 tggcagcgtt ggaggcaaag accgcgcaat acgacatcca cgccctcgct cgtaacatgt
     6421 ccgggttgga agaacgcgcc cgccaggctg agatggccgg cggcgatttc ctttttccac
     6481 gcgtcgagcc gcagaagcgc gggcgagagc aggctgtggc cgaggcggta ggcggctgtg
     6541 gcaaactcat tgctgatggc ggggtccgtg tccggattgt aaccgcgata aggccgcagc
     6601 gcgccagggc cgaggattat gggaagaaat tcacggtagg tgatggcttg cacttctgag
     6661 ccgacgatga ggcgtgcgaa ttgataaatc tcctcctcac cagcatccgg gttcgcagtc
     6721 cggtaaaggt cggcccagaa attgtgctcg cgcacaaaca aagtgtggat ggcgatgagg
     6781 ccaacttgct cgttcacgcg cacgtcacct gcgacgaaga agcccggcac aggcggcagc
     6841 gcggccagct catcagagtg ggtgggcagc agatcaccgt gagcgctgga tgaggtcttg
     6901 agccggccgc ttccgtcgtt ggagcgcaaa gccagcgcgc gcgcttcgtc ggagccgtag
     6961 acctgggagg cgtctatcca cggtgtgatg gcattttttt gctcacgctt cccgttcacc
     7021 acctcatagg cgctccggtt taacccgatc gtggcagcgc ccgtggctgc cggatcaaat
     7081 tgcggatcgc ccgctggaac ctggatggcg aagggctcgg acggagacgc ggccggcgtc
     7141 tcatccagat catgatccag gaactgtccc cactgccaga gcatgtcgct ggccccgcgc
     7201 ccgttgacac gaggcgcggg ctgggcggag accgcgttgc tgatggcgcg cgggctgggc
     7261 cgctgcgcgc ccgcaggctc gcccgcgcca tccgcgtaat cgcccggagc tagacggatg
     7321 aagatccgtc cggcagcgcc gcgttccgta tcggtggcat ggttgcttct gccgtcatgg
     7381 gagcggaact gctcaggcat gacaaatgac tcgggcagcg cgggcggcag cggctgtcgt
     7441 gttttcggag ggggtggatt ttgccttggc ggcggagtct gcggggggcg tggcggctcc
     7501 ggacggccgg gatcggcgcg cgggatgggc gcggctggag tggagttcgc cgaggggcgc
     7561 ggtgggggcg gcctgagctg ccgggtttga gccgtggcgg tgtggatgcc cagggcaaac
     7621 actgccgcgg ccaggagaat cgtttttccg aacggatgga tgttcgtttt catggcctgc
     7681 gaattctttg gttttggtta gtcaagcccg ggtgttccgg agcttgcaca gtcaaggaaa
     7741 agccacccct cctgaagccc ggatgaagat cgcggataaa tttttgaagc gctaaaaaca
     7801 aaacgccgca cccgaaaggc gcggcgttcc ggaggcgaag gccgggcatc agcgcggacg
     7861 cgagcccttg ccacgaccgg gtccgccggg gcctcccgcc ccgcgcaaga ccacgggggt
     7921 gcccctgaat gcgcgcggaa tgaccggcca ttcagcggtg agaaagtagg cgtagacgcc
     7981 ctggggaaag tcgggggtga cgcagaagcg gccattgcat tcgtcaagat cgccatgacc
     8041 ggcgatgaag acataatcct ggacaaaagc tccgtcgtga ggcccgcccg ggcctttcgg
     8101 cgggcgcgga cgctcaccgg gtttgagatc gtaactggag cgcagggttt tcatccccga
     8161 agccggatcc tgcggatctt tgtatccacg cagcgcatag atgggaaaac catccgccgc
     8221 ccagccgatg agaggtgaat gctgatcggc ggcaagtccc aactgatcca tcagcagagt
     8281 tggaaggccg tgataatgat agacgccgcc aggctggaca tgcgcgtggc tggcatcgag
     8341 acccagcggc accgcgccgc cgagcgcctc atagttccag ccaagagcgc ggtcaccctg
     8401 ccaaaactcc gcggtgcccg gatcgaagag tacgccgttg agagcgatgc caaaaggcat
     8461 gtttggcggg ccgcgctcac ctgcgggctg cgtgtgcagc caggttgttt gcgcggcggg
     8521 ttgcgggtcc gccggcaggc ggaaatcgta acgctgggct tcgatgtggt gcggattgcc
     8581 gcggttggga aagcggccga cgaggtgatc cggcagggcg ttggaggtga tgtcgagaga
     8641 gggtccgcgc cgcaggatgc gaacttcgtt ttcacccgga ggcttttcag tggctggcac
     8701 cagagtcagg gcggccgctt ctttcgcgcg gtgctggcgc atcggcgggc cgcctgcgcc
     8761 tggaggaccg cctggacgtt gagcgatcag gatggatgtg acaatgcagg ccaggagaca
     8821 aagagcgcgc gggttcatcc acgcactctc cccgcggcac cttaagccgt catgaagaaa
     8881 gcggggtatc gaaaaacaag gttggacgcc gccgcgccgg gtgtgcatga tgcgcgcatg
     8941 aaagcgtggc ttttccccct gctgctgtgc tgcggcttcg tttccgcgga tgaatccccc
     9001 atcaccctgg tgatccatgg cggcgcggga gtaggcaggg agagcctgac accggacaag
     9061 gaaaaacaat gccgccaagt cttggagcaa gccgtgcgcg aggggcatgc ggtgctggtt
     9121 aaggacggcg cggctttgga ggccgtgcag acggcggtca tgattttgga agactcgccc
     9181 ctgttcaatg ccgggcgcgg ggccgtgctc actgcggcgg gaacggcgga gctggatgct
     9241 tcggtgatgg agggtcgcac gggccaagcc ggcgcggtgg cgggtgtgca gggtgtgcgc
     9301 catcccattc agcttgcgga gatggtaatg cggaagacgc cgcatgtgct catgatcggc
     9361 cggggggctg aagagctggc ccgcgcgcag ggtttgcgat ttgaggatct ggactggttt
     9421 atcacgccgg agcaggtggc gcgcctggag agggcgcgga aaaaatccgc cgcgctagac
     9481 gacaacgatt tcagcctgcg catgggcacc gtcggcgcgg tggcgctcga ccggcaggga
     9541 aacctggccg ccggcacctc gaccggaggt ctggtcaaca agcgcagcgg gcgggtcggc
     9601 gactcgccgg tcattggcgc gggaacttat gcggaaaacg gggtctgcgc cgtctcatgc
     9661 accggccacg gggagttttt catacgcaac gtcgtcgcct atgatgtggc ggcgcgcatg
     9721 aaatacggtg gtgccagcct cgccgacgcg gcggaggccg tcatcaatga aaagctaaaa
     9781 aaactcgatg gccggggcgg cctgatctca gtggatgcca ggggcggcat cgccatgccc
     9841 ttcaacagcg acggcatgtt ccgtgcatgg ataggccccg ataacgtgcc gcatgtggcg
     9901 attttccaag actgacccct gcgtggaaaa cgaatgccat aaaggcgaag gccgcgcctt
     9961 ccggcgcggc cttcgatttt ggtagcgggg acaggatttg aacctgtgac cttcaggtta
    10021 tgagcctgac gagctaccgg gctgctccac cccgcgattg atggcgcaga gtcgtccgga
    10081 tcattgcgaa tgcaaccgca tttttgatgg aagcatgagg gcacgaaaaa gccctgcggg
    10141 gggattcccc gcagggcttt tgatggcgaa tcacttcgcc gtggcagagg tctcttgcga
    10201 gaccctcagc ttatttgcct tttttggcgg gagccttctt cgcaggagcg gcctttttag
    10261 cgggcgcttt cttcggagcg gctttcttgg cgggcgcggc ctttttggcg ggcgctttct
    10321 ttgcagcttt cttagccatg ttgttgttcc tcttgttgtt ggttgctttg ttgttttgtt
    10381 ttgccgagga ggcagcggaa ctgccaccac ggacatcttt tccattggcc gctttttcgg
    10441 cggcgcgctt ctcctcgatc gcggcctggg cggctgccag acgggcgacg ggaacacgga
    10501 agggactgca actgacatag gtcaggccca ccttgtggca gaacttgacg ctgtcggggt
    10561 cgccgccatg ctcgccgcag atgcccagct tgatgccggg gttggtgctg cggccttttt
    10621 cgatggcgat cttgatgagc tggccgacgc cggtctggtc cagcgtggcg aacggattct
    10681 ttttgaagat ctcgttctcg gtgtagggca tgaggaagct gcccatgtcg tctcggctga
    10741 tgccgagcgc cgtctgggtc aggtcgttgg tgccaaaaga gaagaactcc gccgtctggg
    10801 cgatctcgtc ggcggtgagc gcgccgcgag ggacttcgat catggtgccg accatgtagt
    10861 caatcttgac cttcttctcg gccatgacct ccttggcgac gcggaggacg atctcggtct
    10921 gcagatccag ctccttcttg aagccgacca gaggaatcat cacctcaggt ttcaccttga
    10981 tcttcatctt cgccacatca gcggcggctt caaagattgc gcgggcctgc atctccgtga
    11041 tttctggata cgcgatgccc aggcggcagc cgcggtggcc gagcatgggg ttgaactcat
    11101 gcagctgctc gactcggtgc gcgatcttgt cagcgctgat gcccatcttg gaggccagct
    11161 ccttctgctg cttggcagtg tgcggcagga actcgtgaag aggcgggtcc agcaggcgga
    11221 tcgttgcagg caagcctttg agggactcaa aaatgccggt gaagtcttcg cgctgataag
    11281 gcagaagctt ggccagggcg gctcgacggt ccttttcgtt atccgcgaga atcatctcgc
    11341 gcatggcgtc aatgcggtcg ccctcgaaga acatgtgctc cgtgcgggtg agaccgatgc
    11401 cctgcgcgcc gaatgcgatg gcgttctgcg tctgctcagg attgtcggca ttggtgcgca
    11461 cctggaggcg tgtgactttg ccgcaccact tcatgagctg gttgaagtgc tggaacttct
    11521 ccgtgccctg ggcagccttg tcgccgtgca gcatgccggt gatgatttcg gacggcgcgg
    11581 tcttgagctg gccgccatag acggtgccgc tggtgccatc aatgctgagg aagtcgcctt
    11641 ccttgaaggt cttgctggcg acggtgacgg tcttcttgtc gtaattgatc tcaagcgcgg
    11701 aggcgccgca gatgcagacc ttgcccatct gacgcgcgac aagcgcggcg tgggaggaga
    11761 cgccgccctt ggcagtgagg ataccctcgg cggcgatcat gccgcgcaga tcctcggggg
    11821 aggtttcgtt gcggaccagc agcaccttct caccgcgctg ctcggcagcg gcggcacggt
    11881 cggcattgag gtaaatcttg ccggaagccg cgccggggcc ggcgggaagg ccggtggcga
    11941 gcgctttggc gttgcgcacg tcctcgtgat cgaagatcgg ggcgaggagt tggtcgagct
    12001 gctcggcggg attgcgcagg atggcggttt cccagtcgat gaggcgctct ttcaccatgt
    12061 ccatggagaa cttcagcgcg gcggcggcgg tgcgcttgcc gttgcgggtc tgcagcataa
    12121 acagcttgcc ctgctggatg gtgaactcga cgtcctgcac gtctttgaag tgcttttcca
    12181 gaatggcgcg cactttcatc agctcggcgt aagccttcgg catttcagcc tgaagctgcg
    12241 cgacgggctc cggagtgcgc acaccggcga cgacgtcctc gccctgcgcg ttgatgagaa
    12301 actcgccgta aaattcgttg atgccgttgg ctggattgcg ggtgaaggcc acgccggagc
    12361 cggaatcatt gccggtgttg ccatagacca tcgcctgcac gttgacagcg gtgccccact
    12421 cggcggggat gttgtatttg cggcgataga cgatcgcgcg gtcattcatc caggagccga
    12481 acacagcgcc ggcggcgccg cgaagctgat cccacgggct gtcagggaag cccttgccag
    12541 tgcgctcttt gacgagggcc ttgaagcgct tcaccagctc ctgctggtcg gcggcggtga
    12601 ggtcgctgtc cacgatgtcc ttgccataga cctcatgttt gtagccttcg atgacggttt
    12661 caaaaggctc gtggtcttcg ccctcgcgct tctgcacgcc gagcaccacg tcgccataca
    12721 tctgcacaaa gcggcggtag cagtcccagg cgaagcgctc gttgttcgtg gcgctggcga
    12781 gggagaggac ggtctggtcg ttgaggccaa ggttgaggat ggtgtccatc atgcccggca
    12841 tggagtcgcg cgcgccggag cggacggcga cgaggagcgg catgcccttg gcgtcgccaa
    12901 atttgcaggc catgatcttc tccatcttgg cgatgccttc ctccatctgc ttctggagat
    12961 cggcggggta gctgcgcttg ttggcataat aaaacgtgca gacctcggtg gtgatcgtga
    13021 accctggagg aaccggcagg ccgatgcggc tcatctccgc gaggttggcg cctttgccgc
    13081 cgagcagcgc tttcatggag ccatcaccat ctgcttttcc tgctccgaag gaatagacgt
    13141 acttcactgc cttgctgctg ctgttgctcg ctttttttgc catagggggt tgttcttcgt
    13201 gatcgggctt gttgttcttg ttgttatgtt gtcaggagac ggcaccgata tttgtcttcg
    13261 gattatagga tgcaagcatt tgttgacaat agaaagttga gcggccacgc gacacgcgct
    13321 catcaattca tcatgaagat gccatcactg atgatgatga agagcctctc cggtcatgcg
    13381 cgcgtgacgc gccaagtctt gtctgcgcga tgaagatgcg cgctcatcat catgcgcgcc
    13441 atgcgagcgg aaacgcggct agcgctttga cgcggcggcg gttgcgcgcg aaaaaaattc
    13501 ttcgaacaat cacggaggca gccggaacaa atcttgacgg ggagcgcgga ttttgattta
    13561 acgtgtgaaa attcagcgcg tccccagcat caaggagcgc gcgagtttca gaaaaaatcc
    13621 cccatctcaa agccatgaaa acattcgcaa aattccagca cacaacccgc cggggcttca
    13681 ctctgatcga gctgctcgtg gtcatcgtca tcatcgccat ccttgcctct ctggccattc
    13741 ctgtgggcaa tctcgtcatg gacagaacca atcgcctgcg cgtgcaaaaa accatgaagg
    13801 acgtcatcgt cgccgtgaac cactacagga ccgaatacaa ccgcttcccc attggcatca
    13861 acgacgccac tggcggggat tctgacatgg ccccctttct caccgatggg aacggtacgc
    13921 cgatcatcaa catcctcatg gcaaacaccc aggacggcaa cgaggacatg aacccgcgca
    13981 agatcaagtt cgtggacatc cccttcgcgc ggaacaacca gttcggcatc atcagccaga
    14041 gcggcggcgc gaacgacggc gcgcctgtgc agttggtgga cgtctggggg cagccttatt
    14101 cgatcgctct ggacacgaac tatgacaatc tgatcgaaaa ccccgacaaa cagaacaacg
    14161 acctgaccat ctccagccgg gccacggaca atgtttcggc cagcgccatc atgtggagct
    14221 atggcaaaga ccgccagcag gggaccaagg acgacatcgc ctcctggcgt tgagccatcg
    14281 ggcgtctttc attccaagaa aaaagacggg gagaattccc cgtctttttt gtcttcacac
    14341 atatcgcccg gctcgatgtc aggaatatct ggcgagcaga acggaggctt tggcgggttc
    14401 cactttttca tggaaatcat tctccaccat gcacacgggg ccgaaaccgc agctcgccag
    14461 gcattcggcg aactcgatgc tgaacttgcc gtccgcgctc acggcgatgg ggtggtggtg
    14521 gtcgcaatgg ctgcggtcaa tgccggcctc cttgcacagc gcctccatca agtcaaagga
    14581 gccggccatc gcgcatgaga gggtgcggca gacacggatg tggtatttgc cgggcgcagt
    14641 ctggcggaag cctggataga aggtcaccac ttcgagcacc tgtatgggct ccagccccag
    14701 cttggcggcc acccaagcca cgatgtcctc gctgatgaag cggaagtgct cctgcatcag
    14761 atggagcagg ggcagcacgg cgctgcgttt ggacacgggg tagtgcgtga tcacctcgtc
    14821 catcttgctc tctaactcgg cggggactgc gaaagccatc tctctatata taatgtgcgg
    14881 gttcagggcg tgcggtcagc ggtcgcactc gcccatgacg aaatccagac tgccaagcac
    14941 ggcgggaatg tcgctgagca tgtggccttt gagcatcttc ggcaggatgc tcagattgac
    15001 gaaggagggg ctgcggattt tcagccggta ggggacgccg ccgcctttgc tgtgaatgta
    15061 aaagccgagt tcgcccttgg gattttcagc gccgaaataa acctcgcccg cgggcgcgtc
    15121 tatgccctgg gtggcgatga tgaagtggtg gatgagctcc tccattttca tgagcacgcg
    15181 ttctttctgc gggaggagat ttttcgcgtc ggccacattg atcggcccgc cgggcaggct
    15241 ggccagcacc tggcgcagga tgcgcgcgct ttgccgcatc tcctccatgc gcacgaggta
    15301 gcggtcatag cagtcgccgg tggagccgac ggggatgtcg aactcgtagc ggtcgtagtg
    15361 caggtagggg tgcgttttgc gcaggtcgtg atccacaccg gagccgcgca ggttcgggcc
    15421 ggagagacca aaggaaatgg cgtcctcttt cgagatgacg ccgaggccct gggtgcggtt
    15481 gatgaagatc gcgtttcggg tcagcagctt gtctatttca tcaatgacgg gcagcacctc
    15541 gtcgaggaac ttcttcacag cggcggtgaa gccctccggc agatcgcgta tctgcccgcc
    15601 gacgcgcgtg tagctggtgg taaagcgcgc gccagtgagc tgctcgcaca aattatagat
    15661 cttttcgcgc tcggtgaagg tgtagaggaa ggccgtcatc gcgcccacat ccatggcgaa
    15721 gacgccgact ccgagaaggt gggcggaaat gcgcgccagc tcgcagcaga tcacgcggat
    15781 ggcctgcccg cgctcgggca cttcccaccc catgagtttt tccacagcca gggcgtaggc
    15841 gacattgttg gcgagcggtg ccaggtagtc cagccggtcc gtgtagggca cgaactggtt
    15901 gtagtgcatg ttctcggcga tcttttcatc gccacggtgc aggaagccca catccggatc
    15961 tgccttggtg atgacctcgc cgtccatctc cagcaccaga cgcagcacgc cgtgcgtggc
    16021 agggtgggag gggcccatgt tgagcaccag tttctcgccg acgatgtctt cggcggtctc
    16081 ctccatggac tccaggtgcc gcgccgcttt cgcggcggtg tccggggctt cgtgttcgcg
    16141 ggtgaccgtg ggcatggatg tttgggggcg aatttgagta ttctacgccg gggaaaaccc
    16201 agcaaggcga atttgtgaaa aatttcacaa gctcggtgat gacgaagcct ggagctccat
    16261 ttctcggcca tctaaaaccg gtttgtccgc gtggcgaaac ctgccgcccc tgccagcggc
    16321 agcgttttcc gcttgcctcg gcaatgcgaa acgggcgagg gtaacccaaa ttcatgccca
    16381 tgtccgcccg ccttgatgct tctgagtctc ccgtcgtcac cactgcccag gccagggagg
    16441 cggctgaccg gctgggggtt ctggtggccg ggctgtcgga gattatcttg ggaaaagagg
    16501 atgtgctgcg cctggcggtg acctgtctgc tggcgcgcgg gcatttgctc ttcgaggact
    16561 tgcctggcgt gggcaagact ctgctctcgc aggcgttggc gcaggcgctg gggttgcgct
    16621 tcaggcgcgt gcagttcacc agtgacctgc tgccggcgga catcctcggc gcgaacatgt
    16681 atgaccgtca gtcgggtggc tttgtttttc acccggggcc ggtatttgcc caggtactgc
    16741 tggcggatga aatcaaccgc gccacgccca agacccagtc cgctcttttg gaggcgatgg
    16801 aggagggcag ggtgacttgc gatggcgagt ctcacgcgct gccggcaccg tttttcgtca
    16861 tagccacaca aaatccctcc acgcagatgg gcaccttcat gctgccggag agccaactcg
    16921 accgctttct catgcgcctg gccctcggcg tgccggaccg cgcggccgag cgctccatcc
    16981 tccagggtcg ggaccggcgc gaggcgctgc gcgagatgcc cgaactgctc tcctgggagg
    17041 ccatgtctgc catgcagcag caggcgcgtc atgtgcatgt gtcccccgcc cttctggact
    17101 acctgcaaga cctgctggcc gccacgcgcg ccgaagggcg cggcctctcc ccgcgaggcg
    17161 gcctggcg
//
LOCUS       DAOMYJ010000099        17137 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Prosthecobacter sp. isolate AMR_MDS_5629
            NODE_1150_length_17137_cov_22.807868, whole genome shotgun
            sequence.
ACCESSION   DAOMYJ010000099 DAOMYJ010000000
VERSION     DAOMYJ010000099.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867850
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prosthecobacter sp. (wastewater metagenome)
  ORGANISM  Prosthecobacter sp.
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Verrucomicrobiaceae; Prosthecobacter.
REFERENCE   1  (bases 1 to 17137)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17137)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.30
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:34:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,742
            CDSs (total)                      :: 4,684
            Genes (coding)                    :: 4,676
            CDSs (with protein)               :: 4,676
            Genes (RNA)                       :: 58
            tRNAs                             :: 55
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17137
                     /organism="Prosthecobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1150_length_17137_cov_22.807868"
                     /isolate="AMR_MDS_5629"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1965333"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..3232
                     /locus_tag="PLP58_11645"
     CDS             <1..3232
                     /locus_tag="PLP58_11645"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HRJ09217.1"
                     /translation="PAREEDGLALARFEVEAGALIRLCEAGRHEGVVPIHDFKASQTH
                     ACLVMDFIPGLNLRQWCSTHQVSLRERAALIARVARAAGWFHGLGVIHRDLKPANILV
                     HAVTRQPVIVDFSIARMEDSLTLTLTNETLGTAPYMAPEQFDRRRAPATPATDVYALG
                     ASLYELLTQVPPHPGEFAVIVQRHHDEVRPAPPSVLNPAVPRDLESIILKALSHRPAD
                     RYADGTALAEDLERFLAGEPVKARPLPLVTRLVRRARKKPALTAALASSLALLIAAVF
                     ITAHASRQSRQHELESRITQIMRETGWTEGRLMEAEAALSALHSHDPARAAHLADAFL
                     DDVEKDISHALQQPRLTSADEKWMQAAIATLRGRAPELSARLERQMQERQSRWETLVD
                     LKPPFENRAGLFPRGRQSIRGDLLFPDYEDSAGISPSVAVKEGFPTPVEISTTLVSTG
                     PFRHAAFTLVLQKARVDVGLYAAAHAPKDALAAAGGMTPDAGGGVFFILKHGAVESAA
                     HIPEAGLFEQPFHITLRADERGLQAELNQRWTLTSDPVFTFALPSEDNQFRISWARTL
                     GMSSLSIRSRQPGQPSPLEQGDRHAVAGEWSGAQASYEQLVGHPLSGPEALYKLGISQ
                     DKQGDSAAAHLSWRRLADGPPGLWRDLALYQLWRAAVLQQGTTAARPLLDRLPAPGSI
                     PPELTRAIPSADRGKLAKAYARASLGLNFLRVNTAEMDEAMKACAILGIPALETAYQL
                     AGGYHMAGADSMARELANRGLTFPSRQTATPDILGRALGLLDQWGRMDHPESVPELRD
                     RLAEWTRALPQDASFTALTLINQARIHARAGRLEAALPLLESIHALKEVEPRLVTHAM
                     LVEGAVLNALGRQEEARAAWLSGIRSASESATQNPLQLYDRIMMHHATRTWDAGVCDE
                     VINRILGKGKDGLARLTLQGMFLRAFASDPAYVGSLNAFCADPEGRAFIEDYALVRRP
                     ARELFRHWFSRMLEHFILASSLPPGCSPEDRARVRQTISQTLAWISSTEDWMEVMGGF
                     FLAWSSRAPGQMLQAPGGQPPPDLLEKMRWLLDQRSQTPLTPAGGQ"
     gene            3560..4579
                     /locus_tag="PLP58_11650"
     CDS             3560..4579
                     /locus_tag="PLP58_11650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ09218.1"
                     /translation="MQTCHLKLGALTAVIFNASSCVMAPPPGTMIQPDHAVSRTVSQA
                     DGQKWRQHLNSSPFSTMKHGPGFTEAGGTLTRMAGQVRGNAGGMTDPEMRGHSQAVAQ
                     WCLDTERDIRELRAKIAGIRLVAELAEKPAGRVADAFISENGRSTLLGSFIGWTAVNM
                     MRVAEETRQRDQTAARIMEGAHALIRAEQRICREIGVPVPDTLATNLQSVLDSETASI
                     LVGSWQAQNAGALQGMTQHGWQFSSRGTVTVRYADGRVQSGSWRVREGSLLVNWSRGG
                     GSSFFVHVKGWHQVILGGSAQQGTPVLNRFSMKSSDIPVRGPVRSFGRQGRLWINASN
                     LRIRF"
     gene            4587..5438
                     /locus_tag="PLP58_11655"
     CDS             4587..5438
                     /locus_tag="PLP58_11655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ09219.1"
                     /translation="MKSLLLKTLLLLLLPCVPSWAAGAYMRESSNGALIWNNHPAPDD
                     EATWSGGRDDEGYAHGMGTLTWWKRGQWTSKYTGWMNRGLFEGRVENHDSDGEKFAGT
                     FVRGKKQPDWRRVTTAEAVENADAANLPATDADPKEAAKEPAAEAKQTGPMTAGEKDD
                     STDTQVPADPFAHLTHEYKRHLLGAIATNLNPILAAISGGGRDKALAALEELCPASDE
                     NERMAVLIALHHWDSDELIAIRRKSGREAYIRTLYGLALLHHPPVAARPELRRVIEDF
                     LDLVSPF"
     gene            5483..5779
                     /locus_tag="PLP58_11660"
     CDS             5483..5779
                     /locus_tag="PLP58_11660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ09220.1"
                     /translation="MDINFENKARLAKVSCLVGGLAGFHVADMFGMIDLLLPGALVGA
                     VVGYAAPLVKVEAGFAVLLVSCFLILKNCNDAPGGMRAAPETHDRKYDSPPKLE"
     gene            5800..6240
                     /locus_tag="PLP58_11665"
     CDS             5800..6240
                     /locus_tag="PLP58_11665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ09221.1"
                     /translation="MTPTLLIQILDVTHESGREQMQRVRASLPQIEEIAQRHGVRALL
                     ISPHVGEGDPYDAIFAIRMNDDSYSAGRFSAFTAEVEAGTSINAQPLVHSPGNAYLQM
                     LALEQPYTDLLGEKPVNQEEIHAQSGFRRNKTGGSPPPSSTMSA"
     gene            6397..8004
                     /locus_tag="PLP58_11670"
     CDS             6397..8004
                     /locus_tag="PLP58_11670"
                     /inference="COORDINATES: protein motif:HMM:NF025642.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PcfJ domain-containing protein"
                     /protein_id="HRJ09222.1"
                     /translation="MKKHRITHGSRHRPSAARTRHREQRYLDAEVLGRDFVEDEWHTT
                     WDEIWDHHFNLCGSGCCHDHFDTTYKAVLAFLTAPEPLEARVETGVWERISVLEDGGD
                     DCRMDDEEPSLMEPEQLEEGLRLHMRAVHDDKTQVRELLRFSQAELAVLREFRTRTPS
                     GNPARGEISHPVVRMLAWLLVLEPFWLRSPVTWSMPAICDEGAVISLTRHLFVHHPVP
                     ECLLRTPLIEPGPQDGLGWFPWKWFSWLILLGGGGSVRRAASQMGWWTSGEFMRQLHL
                     APAHMTPDEGSLWAEVRRHGGSEALFQAFHDSDAFNLDPTDVPDAALAGRIPDWFRER
                     MAFRRLWQQTLEWMCRHLDELPVEDLSVILTWVSHCHAETGRGRGAAFSFKGRTSASV
                     LRAANMHADEVLAAQEFSLGRLISWQGQGWDACLTDAQEINWTVKELVSNRELAEEGR
                     AMRHCVASYARRCESGLCAVFSVRRGGIRVLTVEVGLPQKEILQIRGSCNRAPHVSEL
                     EVVNQWFAEVAAAPGKHLQTGPYSQAR"
     gene            8023..11973
                     /locus_tag="PLP58_11675"
     CDS             8023..11973
                     /locus_tag="PLP58_11675"
                     /inference="COORDINATES: protein motif:HMM:TIGR02604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="plastocyanin/azurin family copper-binding
                     protein"
                     /protein_id="HRJ09223.1"
                     /translation="MKYLSPVFAALVLCLPVSAQVEFKPGDRIAILGNALPDRAQHFG
                     WLETMLTQANADKDLVFRNLAFAGDEVHTWHRIDGFGTRDEWLTKVGADVIFAFYGFN
                     ESFKGYEGIESFKANLGKFLDETKASNYSGKGAPRIVLFSPFALQKTANPSLPSLEER
                     NTNLQNYTAAMSEVAAAKGAAFVDLYAPTAPGLPAEATLDGLYTTSTGDKAIADAAFR
                     ALTGQSAPTVNEKLRAAILDKNWEWHQRYRTVDGYNVYGGRSGLAYTAGVGGFKQNEK
                     NPEPPYISNYQTMQQEMTQRDVKTANRDLRVHAIAKGGDLVVKDDNLPPVGKVPTNMP
                     DPKEGKEFNKVPMTGMKFTPEGLIAFPDAKEAMKDIQTEPGLKLTLFADEKKFPELVN
                     PVQMAWDTRGRLWVAAWRNYPERTPTSTKGDSLLIFEDTDGDGAADKMTEFVDDLNCP
                     TGFNFYKDGVIVMQSPNLVFIEDTDGDGKGDKRTILLMGLCAADSHHETNALSYDCGG
                     ALYPSDGVFHRTQVETLYGVVRNTNGCIYRFDLNRRELERFTTDATVNPHGKIWDRWG
                     NAYFTDATGNVNTFAEASSCYLPREGGGSVKLKPFWNRPTRPSPGNHVISSRHFPEDW
                     QNNFLNTNVISFQGIWRVPLTQDGAGMKGETEFELVKADPAVYPTFRPSAISVGPDGA
                     LYFCDWSNAIIGHMQHHLRDPNRDKLHGRIYKLTYEGRPLLEPKKIHGESIENLLELL
                     KEWEDNVRQRAKIELAKHDRAQVAAAAKDWAAQLDKNDPEYEHHRLEALWTHQWVDIV
                     DTELLRQVLASPEPRARAAATRVLCYWRDRVPGALDLLKTAVNDPDMRVRMHGVRALS
                     FYRPTPDAEKALEIAYDVLKHPADDYINHVFGECQKGLQSVLKTKVLPKDPAALAEFI
                     SKTSDNDLKSLPDLEPVLIEKLTRPKLPADVRAKALEELAKITGNDSPVVVVDMLKRV
                     EAAAGKLNATADDLGKLLLAQSPGMIARHGLIIKTLAQEAKQAPVKRVCYAAYIVGDK
                     TPDNLWTAVNDGERNYLVESIALIPGSEAALRARFQPHLTALLAGGKTNGGRLRAVLA
                     ALPLTGPENAAANFQLVARHLIEGKERSAAARALLKFPRANWDAALARPVVDNVLAHA
                     KTVPAGNRTRLEYVEVIAAAKEMAALFPEADAKTALDALRGVSVDVFIVRTVHEQLRF
                     DTTRLEVKAGKPFEIVFENDDVMPHNLVIVPPGKHMEIGTAAMTMTPDKLDKQGRAYL
                     PAAFEKEVIAATKLLEPGQQETLKVRAPAQPGEYEYVCTFPGHALIMWGVLSVQ"
     gene            12099..13235
                     /gene="tgt"
                     /locus_tag="PLP58_11680"
     CDS             12099..13235
                     /gene="tgt"
                     /locus_tag="PLP58_11680"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009966088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008479 - queuine tRNA-ribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HRJ09224.1"
                     /translation="MFELLATDTTSAARRGRLRTPHGVVDTPIFMPVGTQGTVKAIHP
                     DELRSLDAHIILGNTYHLWVRPGMEIIREAGGLHAFMNWDRPILTDSGGYQVFSLAKL
                     RKIKEEGCHFNNHVDGTPMFLSPEIAMEIQATLGSDVAMLFDECTPWPCGHEDARKSL
                     ELTLRWARRCREWIDQSQPQTGGGAQLHFGIVQGSTYEDLRQRAARELVAMDFSGYAI
                     GGLSVGEPESDMMRIADWTCPLLPEGKARYAMGLGTPPQMVELIARGIDMFDCVLPTR
                     LARNGTAFTHEGTINLRNNKFARDFRPISDDTHPLCQGFSRAYIRHLINTQEVLGLRL
                     ISLHNLHFYLSLASRARQAIEQGGFAEFRAEVHARYQLRPESPE"
     gene            13232..13576
                     /gene="yajC"
                     /locus_tag="PLP58_11685"
     CDS             13232..13576
                     /gene="yajC"
                     /locus_tag="PLP58_11685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018970312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HRJ09225.1"
                     /translation="MMTSSLFITLLAQAPAGGAPQGGLLGNPLVFMVLMFVMMYFLLI
                     RPQRQRQKEHEKLVSNVKVGDHVAMNGGEHGIITSVKDKTVMLKVADNVKIEYDRSAI
                     ATVTKKSDVVEA"
     gene            13716..16145
                     /gene="secD"
                     /locus_tag="PLP58_11690"
     CDS             13716..16145
                     /gene="secD"
                     /locus_tag="PLP58_11690"
                     /inference="COORDINATES: protein motif:HMM:TIGR01129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecD"
                     /protein_id="HRJ09226.1"
                     /translation="MSPILTFFIGAAILMLLLFYNGTTVHNRRRMLGTVLIVVTSLFA
                     VLSIRNMGIKRGIDLLGGSEFVVQLKPGVDDEGQPKPVASESVQQAIGILQKRLDPEA
                     QTDLLLAPQGEDRILVQMPGVKAEEFDEVRQKIQQVAKLEFRIVHQDSQSKIAEIAAN
                     GGVKEPGWDELKYKVEKDAQGKELPSRGSELVRNRPDMEGKYVSSAFATFDAEGWKVY
                     LNLDSTGAKLFDEIAAANKGRQMAIIVDGEIISAPVLQTDHFGGTAVISGNFKEQEAR
                     VLASLLENPLENPMDILSESAVSATYGEASIRQGIKAGLAGLALVLVFMVFYYRTAGL
                     IAIAGLAVNLLLLFGAMALFQFTLTMPGIAGIALTIGMAVDANVLIYERLREEMAAGK
                     SLAGALESAFEKAFSAIFDSNITTLITAAILFALASGLVKGFAVTLTIGLLTSMFGAL
                     IVTRVLFNWFIDKGILTNLKTATIIPHKVFPILNHAPKFIVASLVITAVSFGALFVKG
                     KHSFGIDFRGGALVHLQLAEGKDLSTEELDAFVKTLKIKDEKSGAESPVGTFYTQKKG
                     SLGEGAMITLRCEFEAGTVIEAGIKGKYPAEVVAGTQVERVGALIGSELAEKSSVALG
                     LAFMFIFIFMAMRFELAFAIGTVVALVHDVMLVPGLCVLLGQEMSVIHVGALLAIAGY
                     SVNDTIIVFDRIREVIKRGGSGGIRDMMNEAISLTLSRTLLTSTTTLFPMIVLLLFGN
                     PAMLEFALPIIIGVFLGTYSSVFIAAPFVLWYARKTGRSLKRQVLEAEEEARKAQAAI
                     AAAKQAQIAGT"
     gene            complement(16272..>17137)
                     /locus_tag="PLP58_11695"
     CDS             complement(16272..>17137)
                     /locus_tag="PLP58_11695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ09227.1"
                     /translation="VLGGRPVGLRRQGSDAPPEVIPWAELAPLAEDALLRLSGRADIV
                     CEDSGAARSWQSRLERWREMDQETRAADFLLHARNSLDTAAVERAAQSLARQTRTLRR
                     MGVLLLAWCFGVISGAYRWLGDGPEVLWLAGGMVLMLWAQAATFWRVARGADSKVRHR
                     FWKTLAITFLPQHAMRAPDEIGSPLRAHPLAARSLLAESEWLRLCREFWKEARHSAAP
                     GSALEARALEEFFSAQDVPLKELEPVPDRHPGAAAWCPRCMAQFQIKDALCMDCGGLE
                     LRAFDSGDRND"
BASE COUNT         3248 a         5824 c         5113 g         2952 t
ORIGIN      
        1 ccccgcccgc gaggaggacg gcctcgcact cgcccgcttc gaggtcgagg ccggcgcgct
       61 catccgcctg tgcgaggccg ggcggcatga gggcgtggtg cccatccatg acttcaaggc
      121 cagccagacc catgcctgcc tggtcatgga ctttatcccc gggctgaacc tgcgccaatg
      181 gtgctccacc caccaggtca gcctgcgcga gcgcgccgcc ctcattgccc gggtggcgcg
      241 cgccgcaggg tggttccacg ggctcggcgt catccaccgc gatctcaagc ccgcgaacat
      301 ccttgtccat gccgtgacgc ggcagcccgt cattgtggac ttctccatcg ccagaatgga
      361 ggacagcctc accctcacgc tgacgaacga gaccctcggc accgcgcctt acatggctcc
      421 cgagcaattt gaccgccgcc gcgcccccgc caccccagcc acggacgtct atgcgctcgg
      481 agccagcctt tacgagttgc tcacccaggt ccccccgcac cccggcgagt tcgccgtcat
      541 cgtccagcgt caccatgacg aggtccgccc cgcgccgccc tccgtgctga accccgctgt
      601 gccgcgtgat ctcgaatcca tcatcctcaa agcgctctcc caccgccccg ctgaccgtta
      661 tgccgacggc accgcgctcg ccgaggacct tgagcgcttc ctcgccggcg agccggtcaa
      721 agcccgcccg ctgccgctgg tcacgcgcct cgtccgccgc gcgcggaaaa agcccgcgct
      781 caccgccgcg ctcgcatcca gtctcgccct cctcatcgcc gcggttttca tcaccgcgca
      841 cgccagccgg cagagccggc agcacgagct ggagagccgg atcacacaga tcatgcggga
      901 gacgggctgg acagaaggcc gcctcatgga ggcagaggcg gcgttgtccg ctctgcattc
      961 gcatgatccc gcgcgcgccg cgcatctcgc ggacgctttt ctcgatgacg tggaaaaaga
     1021 catctcccac gcgctccagc agccccggct cacctccgcc gatgagaaat ggatgcaggc
     1081 agccatcgcc accctgcgcg gccgcgcccc ggagctttcc gcccggctgg aacgccagat
     1141 gcaggagcgc cagtcgcgct gggagacact ggtggacctc aagcccccct tcgaaaaccg
     1201 cgccgggctc ttcccgcgcg gacgccagag catccggggg gatctgcttt ttccagatta
     1261 cgaggacagc gcgggcatct ccccgagcgt ggcggtgaag gaggggtttc ccacgccggt
     1321 ggaaatcagc accaccctgg tctccaccgg gcctttcagg catgcggcgt tcaccctggt
     1381 gctccagaaa gcgcgtgtgg atgtggggct gtacgctgcg gcccacgcgc cgaaagacgc
     1441 tctggcggcg gcgggcggca tgacgccgga cgcgggcggc ggcgtgtttt tcatcctcaa
     1501 acatggcgcg gtggaaagcg cggcgcatat ccccgaagcc gggctctttg agcagccttt
     1561 tcacatcacc ctgcgcgccg atgagcgcgg cctgcaggcg gagctcaacc agcgctggac
     1621 cctcacttcc gacccggtct tcaccttcgc cctgccctcg gaggacaacc aattccgcat
     1681 ctcctgggcc aggacgctcg gcatgagcag cctcagcatc cgcagccggc agccgggcca
     1741 gcccagcccg cttgagcagg gggaccgcca tgcggtggcg ggagagtggt ccggcgcgca
     1801 ggccagttac gagcagctcg tcgggcatcc tctcagcggg ccggaggcgc tgtacaaact
     1861 gggcatctca caggacaagc agggcgactc tgccgccgcc catctgtcct ggcggcgcct
     1921 ggcggacggg ccgcccggcc tgtggcgcga cctcgccctc taccagcttt ggcgcgccgc
     1981 agtcctgcag cagggaacca ccgccgccag accgctgctc gaccgcctgc ccgccccagg
     2041 ctccatcccg ccggaactaa cccgcgccat cccctcagcc gaccgtggaa agctcgccaa
     2101 agcttatgcg cgcgccagtc tcggcctcaa ttttcttcgc gtgaacaccg cggagatgga
     2161 cgaggcgatg aaagcctgcg ccatcctcgg catccccgcg ctggagaccg cctaccagct
     2221 cgccggcggc taccacatgg ccggggcgga cagcatggcg cgggagctgg caaaccgcgg
     2281 cctgaccttt ccctcccggc agactgccac gccggacatc ctcggcaggg cgctcggcct
     2341 ccttgatcaa tggggccgca tggatcaccc cgaaagcgtc ccggagctgc gcgaccgtct
     2401 cgccgaatgg acgcgcgccc tcccccagga cgcaagcttc accgccttga ccctgatcaa
     2461 tcaggcccgc atccatgccc gtgccgggcg gctggaggcg gcgctgccgc tcctggaaag
     2521 catccatgcg ctcaaggaag tggagccccg cctcgtcacc cacgccatgt tggtcgaagg
     2581 ggccgtgctg aatgctctgg gccgccagga ggaggcccgc gccgcctggc tctccggcat
     2641 ccgctccgcc tccgaatccg ccacccagaa cccgctccag ctttacgacc gcatcatgat
     2701 gcaccacgcc acccgcacct gggatgccgg cgtttgtgac gaggtcatca accgcatcct
     2761 gggcaagggc aaggacggcc tggcccgcct cacgctccag ggcatgttcc tgcgcgcctt
     2821 cgccagtgat cccgcctatg tcggctcgct caacgccttc tgcgctgacc ccgaaggccg
     2881 cgccttcatc gaggactacg ccctggttcg cagacccgct cgcgagctgt tccgccactg
     2941 gttctcccgg atgctggagc acttcatcct cgcctcctcc ctcccgcccg gctgcagtcc
     3001 ggaggaccgg gcgcgagtgc gccagaccat cagccaaacc ctggcatgga tctccagcac
     3061 cgaagactgg atggaggtca tgggcggctt cttcctggcc tggagcagcc gcgccccagg
     3121 ccaaatgctc caggccccgg gcggccagcc cccgcccgat cttctggaaa aaatgcgctg
     3181 gctgctcgat cagcgcagcc agaccccgct gacgcccgca ggcgggcaat gaaaatgagc
     3241 cgtgcccggc aggcacagca ccgccccagg agaatcaggg gagatgcggc tcaagtgcca
     3301 gatctccccc ccggacagcc gcctcctgcc gcgaaaaata ttttttcacc cgcactatca
     3361 tcaactccgc ccaaaaagag tagcagcaga tgacaccccg cgtgtctcca atgcactccg
     3421 cccgccgcca gaccccttcc gaaagccccc gtgccgcgca ccccggcggg cgcgcgctgc
     3481 gggaaacccg tctccaagac ccatgcgcgg cctatttcag aaaaaatcca ggcaaaatca
     3541 gatccacaaa aatcaagcca tgcagacctg ccatcttaaa ctcggcgcgt tgaccgctgt
     3601 catcttcaat gccagttcat gcgtgatggc tcccccgcca ggcaccatga ttcagcccga
     3661 ccatgccgtc tcccgcactg tctcacaggc ggacgggcaa aagtggcgtc aacatttgaa
     3721 ctcgagtccg ttctccacca tgaagcacgg ccccggtttc accgaagccg gaggcacatt
     3781 gacccgcatg gccgggcagg tgaggggaaa cgcgggcggc atgacagacc cggagatgcg
     3841 cggtcattcc caagccgtgg cgcagtggtg cctggacacg gaaagggaca tccgtgaact
     3901 gcgcgccaaa atcgccggga tcaggctggt ggctgaactc gcggagaagc ccgccgggcg
     3961 cgtggcggat gccttcatct ccgagaacgg caggtcaacc cttcttggct ccttcatcgg
     4021 ctggacggcg gtgaacatga tgcgtgtggc cgaggagacc cggcagcgcg accagacggc
     4081 ggcgaggatc atggaggggg cgcacgcgct gatacgcgcc gagcagcgta tttgccgcga
     4141 gattggcgtc cctgtgccag acacactggc caccaatctg caatcggtgc tggactcgga
     4201 gacggcatcc atccttgtcg gctcctggca ggcgcagaat gcgggcgcgc tccagggcat
     4261 gacccagcac ggctggcagt tttcctcgcg cggcaccgtg actgtccgtt atgcggatgg
     4321 ccgcgttcag agcggctcct ggcgcgtgcg ggaggggagc cttctcgtga attggagccg
     4381 gggtggaggg agcagcttct ttgtccatgt caaaggctgg catcaagtca tcctcggcgg
     4441 ctcggcccag cagggcactc ctgtccttaa ccgcttctcc atgaaatcat ccgacattcc
     4501 cgtgcggggt cccgtgcgca gtttcgggcg gcagggccgc ctttggatca acgcatcaaa
     4561 tctgcgcatc cgcttctgat atagccatga aaagtctgct tctgaaaact ttgctgcttc
     4621 tccttctgcc atgcgtaccg tcatgggcag caggagccta catgcgcgaa tcgtcaaacg
     4681 gtgcgctgat ctggaacaac catccggcac ccgacgacga agccacatgg tctggcggac
     4741 gcgacgacga aggttacgcc cacgggatgg gcacgctgac atggtggaag cgtggacagt
     4801 ggacttccaa atacacaggc tggatgaatc gcggcctctt cgaaggcaga gtggagaatc
     4861 acgactctga cggcgaaaag tttgccggga catttgtgcg gggcaaaaaa cagcctgatt
     4921 ggaggcgggt gaccactgcg gaagccgttg aaaatgcaga cgctgcgaat cttccggcaa
     4981 cggacgccga ccccaaagaa gcggccaagg aaccggccgc cgaggccaag cagaccggac
     5041 ccatgactgc gggggagaaa gatgactcga ccgacactca agttccggct gatcccttcg
     5101 cccatcttac gcatgagtat aagcgccatc tgctgggggc cattgccaca aatctgaatc
     5161 caatacttgc cgccatttcc ggagggggcc gggacaaggc gctcgccgcg ttggaggaac
     5221 tgtgcccggc gagcgacgag aacgagcgca tggcggtgct cattgccctg catcactggg
     5281 acagtgatga actaatcgcc attcggagaa aatctggaag ggaagcctac atcaggacac
     5341 tctacggcct ggcacttctg caccaccccc cggttgccgc gcgcccggag ctgcgccggg
     5401 tcattgaaga cttccttgat ctggtttcac cattctaact ttggcaccaa cagagggaca
     5461 accattttcc cgatttaaga gcatggacat caacttcgaa aacaaggcgc gccttgccaa
     5521 agtttcatgc ctggtaggcg gactagcggg ttttcatgtt gcggacatgt ttggcatgat
     5581 tgacctgctt ttgcccggtg ctctcgtggg tgcggtggtg ggttacgcgg cccctcttgt
     5641 caaggtggag gcgggttttg cagtgctgct tgtgtcgtgc tttctaatcc tgaaaaactg
     5701 caatgatgct cccggcggca tgcgcgctgc gcctgagaca cacgaccgca agtatgattc
     5761 gccacccaag ctcgaatagc cttctctcaa aattcgatca tgacacccac cctcctaatt
     5821 caaatccttg atgtcaccca tgagtccggg cgggagcaga tgcaaagggt cagggccagc
     5881 ctgcctcaga tcgaagagat cgcgcaaagg catggtgtca gagcactcct gatttcccct
     5941 catgtgggtg aaggagatcc ttatgacgcc attttcgcca tccgcatgaa tgacgattca
     6001 tactccgctg gcagattcag cgccttcacc gcagaagtgg aagccggaac ctccatcaac
     6061 gcccagcctc ttgtgcattc tccgggcaac gcctatttgc aaatgctggc gctggagcag
     6121 ccctacacag acttgctggg agaaaagccc gtcaaccagg aggaaatcca tgcccaaagc
     6181 ggcttcagaa ggaacaaaac ggggggatct ccgccgccat cctcgaccat gtccgcttga
     6241 gactggaatg gagcgcgcgc ttcaccaaga gctggctggt tatgtgaggg cgtttgatcc
     6301 gaggacagcg ttgcggtagt ttttgccgcg agaacgcgcc ccctctgcgg gcagtgttgg
     6361 cgcaggcgag cacgccaccg tattttatcc caccgcatga aaaaacaccg catcactcac
     6421 ggcagccgcc accgtccgag cgcggcgcgc acacgccacc gggagcagcg ctacctggat
     6481 gcggaggtgc tgggcaggga ttttgtggag gatgaatggc acacaacctg ggatgaaatc
     6541 tgggaccacc acttcaatct ctgcggaagc ggctgctgtc atgaccactt cgacacgacc
     6601 tacaaggcgg tcctcgcctt cctgaccgcg ccggagccgc tggaagcgcg ggtggagact
     6661 ggagtctggg agcggatttc cgtgctggag gatggcgggg atgactgccg catggatgat
     6721 gaggagccgt cattgatgga gccggagcag ttggaggaag gactgcgcct gcacatgcgt
     6781 gccgtgcatg atgacaagac gcaggtgcgg gagctgctgc gcttctcaca ggcggagctg
     6841 gctgtgctgc gggagttccg cacgcggacc cccagcggga atccggccag gggtgagatc
     6901 tcccatcctg ttgtgagaat gctggcgtgg ctcctggttt tggagccctt ctggctgcgc
     6961 tctccggtga cttggtcaat gcccgccatc tgtgatgaag gcgccgtgat ttcgctcaca
     7021 cgtcatcttt tcgtccatca tcccgtgccg gagtgcctgc tgcggacgcc tttgatcgag
     7081 cctggtccgc aggacggcct ggggtggttt ccctggaagt ggttttcctg gctgatcctg
     7141 ctgggcggcg gcggcagcgt gcgccgtgcg gcaagccaga tgggatggtg gacgagcggg
     7201 gagttcatgc ggcagttgca cctggccccc gcgcacatga cgccggatga gggcagcttg
     7261 tgggcggaag tgcgcaggca tggcggatcg gaagcgctgt ttcaggcatt ccacgacagt
     7321 gacgctttca acctggatcc caccgatgtg ccggatgccg cgctggccgg caggatcccg
     7381 gactggtttc gggagcggat ggctttccgc aggctctggc agcagaccct ggaatggatg
     7441 tgccgtcatt tggacgaact gccggttgag gatttgagcg tgattttgac ctgggtctcc
     7501 cattgccacg cggaaacagg ccgggggaga ggcgctgcct tttccttcaa ggggcggacg
     7561 tccgcgtcag tgctgcgggc ggcgaacatg cacgcggacg aggtactggc ggcgcaggaa
     7621 ttttcgctcg gtagattgat ctcctggcag ggacaagggt gggatgcgtg tctgactgac
     7681 gcgcaggaga tcaactggac ggtgaaggaa ctggtgagca accgagaact cgcggaagaa
     7741 ggccgcgcga tgcggcactg cgtggcttcg tatgcgcggc gatgcgagtc aggactgtgc
     7801 gccgtttttt cggtgcgcag aggcgggatt cgcgtcctga ccgtcgaagt gggacttcct
     7861 caaaaggaga ttctgcaaat ccgcggctcc tgcaaccgcg cacctcatgt ttctgaattg
     7921 gaggtggtaa accagtggtt tgccgaagtt gccgccgcgc caggaaagca tctccagact
     7981 ggaccgtatt cccaggcccg ctagtctgcg gctttcctga ccatgaaata tctgtcccct
     8041 gtctttgctg ccctcgtctt gtgcctgcct gtctccgccc aggtcgaatt caagcccggc
     8101 gaccgcatcg ccatcctcgg caacgccctg ccggatcgcg cccagcactt cggctggctg
     8161 gagacgatgc tcacccaggc gaatgcggac aaggacctcg tcttccgcaa cctcgccttc
     8221 gccggcgacg aggtccacac ctggcatcgc attgacggct ttggcacgcg tgacgagtgg
     8281 ctcaccaaag tgggcgcgga cgtcatcttc gccttctatg gcttcaacga atccttcaag
     8341 ggctacgagg gcatcgagag cttcaaggcg aacctcggca agttcctcga cgaaacgaag
     8401 gccagcaact acagcggcaa aggcgcgccg cgcatcgtgc tgttctcccc tttcgctttg
     8461 caaaagacgg caaatcccag cctgccgtcg ttggaggaaa ggaacacgaa tctgcaaaat
     8521 tacaccgccg ccatgagcga ggtggccgcc gcgaagggcg ccgcttttgt ggacctctac
     8581 gcccccaccg cgccgggcct gcctgccgag gccacgctcg acggccttta caccaccagc
     8641 acgggcgaca aagccatcgc cgatgccgcc ttccgcgcgc tcaccggcca gagcgcgccg
     8701 acggtgaacg agaagctgcg cgccgccatc ctcgacaaga actgggagtg gcaccagcgc
     8761 taccgcacgg tggatggcta caacgtctat ggcggccgct ccggcctggc ctacaccgcc
     8821 ggcgtcggcg gcttcaagca gaacgaaaag aaccccgagc cgccctacat ctccaactac
     8881 cagaccatgc agcaggagat gacgcagcgc gatgtgaaga ccgccaaccg cgatctgcgc
     8941 gtgcacgcca tcgccaaagg cggcgacctc gttgtgaagg acgacaacct gccgcctgtc
     9001 ggcaaagtgc ccacgaacat gcccgacccg aaagagggca aggagttcaa caaagtgccc
     9061 atgaccggca tgaagttcac accggagggc ctcatcgcct tccccgatgc caaggaggct
     9121 atgaaagaca tccagactga gccgggcctg aagctcacgc ttttcgccga cgagaaaaaa
     9181 ttccccgaac tggtgaaccc ggtgcagatg gcctgggaca cccgcggccg cctctgggtc
     9241 gccgcctggc gcaactaccc cgagcgcacg cccaccagca cgaagggcga cagcctgctc
     9301 atctttgagg acaccgatgg cgatggcgct gccgacaaga tgaccgagtt cgtggacgat
     9361 ctgaactgcc ccaccggctt caacttctac aaggacggcg tcatcgtcat gcagtcgccc
     9421 aacctcgtct tcatcgagga caccgacggc gacggcaaag gcgacaagcg caccatcctg
     9481 ctgatgggct tgtgcgcagc ggattcgcat cacgagacca atgcgctgag ctacgactgc
     9541 ggcggcgcgc tttacccgag cgacggcgtc ttccaccgca cccaggtcga gacgctttac
     9601 ggcgtcgtgc gcaacaccaa cggctgcatc taccgcttcg acctcaaccg ccgcgaactg
     9661 gagcgcttca ccaccgacgc cacggtcaat ccccacggga aaatttggga ccgctggggc
     9721 aacgcctact tcaccgatgc caccggcaac gtgaacacct tcgccgaggc atcctcctgc
     9781 tatctgccgc gcgaaggcgg cggcagcgtc aaattgaagc cgttctggaa ccgccccacg
     9841 cgtcccagcc ccggcaatca tgtcatctcc agccgccact ttcccgagga ctggcagaac
     9901 aacttcctca acacgaatgt catcagcttc cagggcatct ggcgcgtgcc cctcacccag
     9961 gacggcgcgg gcatgaaggg cgagaccgag ttcgaactcg tcaaagccga ccccgctgtg
    10021 tatcccacct tccgccccag cgccatcagc gtcggcccgg acggcgcgct gtatttttgc
    10081 gactggtcca acgccatcat cggccacatg cagcatcacc tgcgcgaccc caaccgcgac
    10141 aagctgcacg gccgcatcta caagctgacc tatgaggggc ggccgctgct ggagccgaaa
    10201 aagatccacg gcgaaagcat cgaaaacctc ctggaactgc tcaaggaatg ggaggacaac
    10261 gtccgccagc gcgccaagat cgaactcgcc aaacacgaca gggcgcaagt ggccgccgcc
    10321 gccaaggact gggccgccca actcgacaag aacgaccccg aatacgagca ccatcgtctc
    10381 gaagccctgt ggacccatca atgggtggac atcgtggaca ccgaactcct caggcaagtc
    10441 ctcgcctccc ccgaaccccg cgcacgcgcc gccgccaccc gcgtgctttg ctactggcgc
    10501 gaccgcgtgc ccggcgcgct cgacctgctc aagactgccg tcaacgatcc ggacatgcgc
    10561 gtgcgcatgc atggcgtgcg cgcattgagc ttctaccggc ccacccccga cgctgaaaag
    10621 gccctcgaaa tcgcttatga tgtgctcaag caccccgccg atgactatat caaccatgtc
    10681 ttcggcgagt gccagaaggg actgcaatcc gtgctcaaga ccaaagtcct gcccaaagac
    10741 cccgccgccc tagccgagtt cattagcaag accagcgaca acgacttgaa aagtctgccc
    10801 gacctggagc cggtgctcat cgaaaaactc acccgcccga aactgcccgc cgacgtgcgc
    10861 gccaaggccc tggaggaatt ggccaaaatt accggcaacg attcgcccgt cgtggtcgtg
    10921 gacatgctca agcgcgtcga ggccgctgct ggcaagctca acgccaccgc cgacgatctc
    10981 ggtaaactcc tgcttgcgca gtctcccggc atgatcgccc gccacggtct catcatcaaa
    11041 acactcgccc aggaggcaaa acaagccccg gtgaagcgcg tctgctacgc cgcctacatc
    11101 gtcggcgaca aaacacccga caacctttgg accgccgtca acgatggcga gcgcaattat
    11161 ctcgtcgagt caatcgccct cattcctggc agcgaggcag ccctacgcgc caggttccag
    11221 ccgcacctga cggcgctgct tgccggcgga aaaaccaacg gcggacgctt gcgcgccgtg
    11281 ctcgccgcgc tgccgctgac tgggccggag aatgcggctg cgaatttcca gcttgtcgcg
    11341 cggcatctca tcgaaggcaa agagcgcagc gccgcagcgc gtgctctatt aaaatttccc
    11401 cgcgccaact gggacgccgc tctggcccgg cccgtggtgg acaatgtgct cgcccacgcc
    11461 aaaaccgtgc ccgctggcaa ccgcacccgg ctcgagtatg tggaagtcat cgccgccgcc
    11521 aaggaaatgg ccgccctgtt tcccgaagcc gacgccaaaa ccgctctcga cgctctgcgc
    11581 ggcgtcagcg tggatgtctt catcgtgcgc accgtgcatg agcagctccg cttcgacacc
    11641 acgcggcttg aagtcaaagc gggcaaaccc ttcgagattg tcttcgaaaa cgacgacgtc
    11701 atgccgcaca atctggttat tgtcccgcct ggcaaacaca tggagatcgg caccgccgcc
    11761 atgaccatga cccccgacaa gctggacaag caaggccgcg cctacctgcc cgccgctttt
    11821 gaaaaagagg tcatcgccgc caccaagctt ctggagcccg gccagcaaga gaccctcaaa
    11881 gtccgcgccc ccgcccagcc tggcgaatac gaatacgtct gcacctttcc cggccacgcc
    11941 ctcatcatgt ggggcgtgct gagtgtgcaa taagctcgat tttttttcca gccccacgct
    12001 tagccgtccc cacgcagtgt agagggtcgt cgcgtgagac gcgcagcctt tgacaggccg
    12061 ggcatccgcc gccacactcc gcgccctttt gcccgtccat gtttgagctt ctggccactg
    12121 acaccacctc cgccgccagg cgggggcggc tgcgcacgcc tcatggagtg gtggatacac
    12181 ccatcttcat gcccgtgggc acgcagggca cggtgaaggc catccatccc gatgaactgc
    12241 gctccctgga cgcgcacatc attctcggaa acacctacca cctctgggtg cggcccggca
    12301 tggaaatcat ccgcgaggcg ggcggcctgc acgccttcat gaactgggac cggcccatcc
    12361 tcaccgacag cggcggctac caggtcttct ctttggcaaa gctgcgcaag atcaaggagg
    12421 agggctgcca ttttaacaat catgtggacg gcacgccgat gttcctcagt ccggagatcg
    12481 ccatggaaat ccaggccacc ctgggcagcg acgtggccat gctcttcgat gaatgcacgc
    12541 cctggccctg cggccatgag gacgcgcgca agagcctgga gctgaccctg cgctgggcgc
    12601 ggcgctgccg ggagtggatt gaccaaagcc agccgcagac cggcggcggc gcgcagcttc
    12661 acttcggcat cgtccagggc agcacctatg aggacctccg ccagcgcgcc gcgcgcgagc
    12721 tggtggcgat ggacttcagc ggctacgcca tcggcggcct gagcgtgggc gagccggagt
    12781 cggacatgat gcgcatcgcc gactggacgt gcccgctgct gccggagggc aaggcgcgct
    12841 acgccatggg cctgggcacg ccgccgcaga tggtggagct catcgcgcgc ggcatagaca
    12901 tgtttgactg cgtgctgccc acccggctgg cgcgcaacgg caccgccttc acgcacgagg
    12961 gcaccatcaa cctgcgcaac aacaagttcg ccagggattt ccgccccatc tcggacgaca
    13021 cgcacccgct ctgccagggc ttttcccgcg cctacatccg gcatctgatc aacactcagg
    13081 aggtgctggg tttgcggttg atttccctgc acaatctgca tttctacctg tccctcgcgt
    13141 cccgggcgcg tcaggccatc gagcaaggtg gcttcgccga attccgcgcc gaggttcacg
    13201 cccgttacca gctccgtccc gaatcccccg aatgatgaca tcctccctct ttatcaccct
    13261 tctcgcccag gcgcccgctg gcggcgcgcc tcaaggcggc ctgcttggca atccgctggt
    13321 cttcatggtg ctcatgtttg tcatgatgta cttcctgctc atccgccccc agcgccagcg
    13381 ccagaaggag catgagaagc tcgtctcgaa tgtcaaagtc ggcgaccatg tggccatgaa
    13441 cggtggcgag cacggcatca tcaccagcgt caaggacaag accgtcatgc tcaaagtggc
    13501 cgacaacgtg aagatcgaat acgaccgctc cgccattgcc actgtgacca agaagtctga
    13561 tgtcgtcgag gcctgagcgc gtgaaattcg aagttcggaa ctcgaaaacg tccgtcgtct
    13621 gccgcgttga accgccccct cattccacct ctgcccccaa ctaaccccaa cccccttcga
    13681 gcttcgattt tcggacttcg gatttcctcc tcctcatgtc acccattctg accttcttca
    13741 tcggcgcggc catcttgatg ctgctgctct tttacaatgg caccaccgtt cacaatcgcc
    13801 gccggatgct gggcactgtg ctcatcgtgg taaccagcct ctttgccgta ctctccatca
    13861 gaaacatggg catcaagcgc ggcattgacc tcctgggcgg cagcgaattc gtcgtccagc
    13921 tcaagccggg tgtggatgat gagggccagc ccaagccggt cgccagtgag tccgtgcagc
    13981 aggccattgg cattctgcaa aagcgccttg accccgaggc tcagacggac ctcctgctcg
    14041 ccccgcaggg ggaggaccgc atcctggtgc agatgccggg tgtgaaggcg gaggagtttg
    14101 acgaagtgcg ccagaagatc cagcaggtgg cgaagctgga gttccgcatc gtgcatcagg
    14161 acagccagtc aaagatcgcc gagatcgccg cgaacggcgg agtcaaagag ccgggctggg
    14221 acgagttgaa gtacaaggtg gaaaaagacg cccaaggcaa ggagctgccc tcccgtggca
    14281 gcgagctggt gcgcaaccgg ccggacatgg agggcaagta cgtcagcagc gccttcgcca
    14341 cctttgatgc cgagggttgg aaggtctatc tgaacctcga cagcaccggc gcgaagcttt
    14401 tcgacgagat tgccgccgcc aacaaaggcc gccagatggc catcatcgtg gacggcgaga
    14461 tcatctccgc tcccgtgctg cagactgacc acttcggcgg cactgccgtc atcagcggca
    14521 acttcaagga gcaggaggcg cgcgtgctcg ccagcctgct ggaaaacccg ctggagaacc
    14581 ccatggacat cctctccgag agcgccgtct ccgccaccta cggcgaggct tccatccgcc
    14641 agggcatcaa ggcaggtctc gcaggtttgg cgctcgtgct cgtgttcatg gttttttatt
    14701 accgaacggc aggactcatc gccattgccg gtctggcggt gaatcttctg ttgctgttcg
    14761 gtgccatggc gcttttccag ttcacactga ccatgcccgg catcgccggc atcgcactga
    14821 ccatcggcat ggcggtggat gcgaacgtgc tcatttacga gcgcttgcgg gaggaaatgg
    14881 ctgccggcaa atcactggcc ggggcgctgg agtccgcgtt tgagaaggcc ttctccgcca
    14941 ttttcgactc gaacattacc accctcatca cggcggcgat cctctttgcc ctggcgagcg
    15001 gcttggtgaa gggcttcgcg gtcacgctta ccatcggtct gctgacctcc atgttcggcg
    15061 cgctcatcgt cacccgcgtg cttttcaact ggttcattga caagggcatc ctgacgaacc
    15121 tgaagaccgc cacgatcatc ccgcacaagg tcttccccat cctcaaccac gcgccgaagt
    15181 tcatcgtcgc gtccctggtc atcaccgcgg tgtccttcgg ggccttgttt gtgaagggca
    15241 agcacagctt cggcattgac ttccgcggcg gcgcgctggt ccacctccag ctcgccgagg
    15301 gcaaggacct ctccaccgaa gagctcgacg cctttgtgaa gaccctcaag atcaaggacg
    15361 agaagagcgg cgccgagtcc cctgtgggca ccttttacac ccagaaaaaa ggctccctgg
    15421 gagagggtgc catgatcact ctgcgctgcg agttcgaggc cggcaccgtc atcgaggccg
    15481 gcatcaaggg caagtatccc gccgaggtcg tcgccggcac ccaggtggag cgcgtgggcg
    15541 ccctcatcgg cagcgagctg gcggagaagt cctccgtggc gctcggcctg gccttcatgt
    15601 tcatcttcat tttcatggcc atgcgcttcg agctggcctt cgccatcggc accgtcgtgg
    15661 ccctggtgca tgacgtgatg ctcgtgcccg gcctgtgcgt gctgctcggc caggagatgt
    15721 ccgtcatcca cgtcggcgcg ctgcttgcca tcgccggtta ctcggtcaat gacaccatca
    15781 tcgtcttcga ccgcatccgc gaggtcatca agcgcggcgg cagcggcggc atccgcgaca
    15841 tgatgaacga ggccatctcc ctcacactct cccgcaccct gctgaccagc accacgaccc
    15901 tcttccccat gatcgtgctg ttgctgttcg gaaacccggc catgctcgag tttgccctgc
    15961 ccatcatcat tggcgtcttc ctgggcactt attcctcggt cttcatcgcc gcgcccttcg
    16021 tgctctggta tgcgcgcaag accggccgca gcctcaagcg ccaggtgctc gaggccgagg
    16081 aggaggcgcg caaggcccag gccgccatcg ccgcagcgaa gcaggcccag atcgccggga
    16141 cctgattccg ccagtgtcgt tttggaaaaa gcccgcctct ccctcagggg aggcgggttt
    16201 tttgcggatg gcaacccgcg agggtgcgca aacatgagtc tccgtgaagg ggagttgcct
    16261 cggtcattcg gtcagtcgtt tctgtcgcct gaatcaaaag cgcgcagttc caaaccgccg
    16321 caatccatgc agagggcgtc ctttatttga aactgcgcca tgcagcgcgg gcaccaggca
    16381 gccgcgccgg ggtggcggtc cggcacaggc tccaattcct tcagtggcac atcctgggca
    16441 ctgaaaaact cctccagcgc gcgcgcctcc agtgccgagc cgggcgcggc gctgtgacga
    16501 gcctccttcc aaaactcacg gcacagccgc agccactcgg actccgcgag cagggaccgc
    16561 gccgccagcg gatgcgccct cagcgggctg ccgatctcat ccggcgcgcg catggcatgc
    16621 tggggcagga aggtgatggc gagcgtcttc cagaaccggt ggcgcacctt ggagtccgcg
    16681 ccgcgcgcca cgcgccagaa ggtcgccgcc tgcgcccaaa gcatgagcac catgccgccc
    16741 gccagccaga gcacctccgg cccgtctccc agccagcggt aagcgcccga gatgacaccg
    16801 aagcaccaag cgagcagcag cactcccatg cggcgcagcg tccgtgtctg ccgggcaagg
    16861 gattgggcgg cccgctccac cgcggctgtg tccagactgt tcctggcatg gagcagaaaa
    16921 tccgccgcgc gcgtttcctg gtccatttcc cgccagcgct ccagccttga ctgccaggag
    16981 cgcgcggccc cggagtcctc gcagacgatg tctgcgcgcc cgctcaggcg cagcagcgcg
    17041 tcctccgcca gaggggcgag ctccgcccag gggatcacct ccggcggggc gtcacttcct
    17101 tgccgacgaa ggccgacagg tcgaccaccg agtacag
//
LOCUS       DAOMYJ010000100        17061 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Prosthecobacter sp. isolate AMR_MDS_5629
            NODE_1157_length_17061_cov_20.802540, whole genome shotgun
            sequence.
ACCESSION   DAOMYJ010000100 DAOMYJ010000000
VERSION     DAOMYJ010000100.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867850
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prosthecobacter sp. (wastewater metagenome)
  ORGANISM  Prosthecobacter sp.
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Verrucomicrobiaceae; Prosthecobacter.
REFERENCE   1  (bases 1 to 17061)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17061)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.30
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:34:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,742
            CDSs (total)                      :: 4,684
            Genes (coding)                    :: 4,676
            CDSs (with protein)               :: 4,676
            Genes (RNA)                       :: 58
            tRNAs                             :: 55
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17061
                     /organism="Prosthecobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1157_length_17061_cov_20.802540"
                     /isolate="AMR_MDS_5629"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1965333"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1514
                     /locus_tag="PLP58_11700"
     CDS             <1..1514
                     /locus_tag="PLP58_11700"
                     /inference="COORDINATES: protein motif:HMM:NF012437.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HRJ09228.1"
                     /translation="SAGPPRPPGVSLGAPFIGLLDKEGNFISSSRGRQGNPLVEPRTG
                     NAGVEGEEMELRRRSLKMPWLGGRDFESVLRKQEAGYLILEAGGRPQVREVFITPLRS
                     PEDGSLLGAFAFGLPLPALAERLLYDQTRRGDNGEIMSGVYLEGRLVSSTLPEARRTV
                     VEGLLEAALAGAGDAGSEIITLIGEVRHRLIYRVLNPDSPFPKAVQVSFYPLSALDEE
                     IRGLRKGTAGLALAALLVSLGVVLAVSRGLSGPVNRLVAAVHEIEQGNFEMRVLVTPD
                     ETGRLARALNQMAAGLALQEKYRSVLNAVADRAVAERLIENREALSGELRSVSVLFCD
                     IRGFTALSERMPPQELIVLLNTHMTTMTRLAYEHGGIVDKFVGDLIMVLFGAPVSTGR
                     DAEQAVACARAMLAERRRMNESGEGMPLEVGIGIATGDVVAGCMGSDQRLSYTVLGHR
                     VNLASRLCSVAGPGEIMTDAETMRALGEGARVEAMPPMRLKGMSDPVEVFRLG"
     gene            complement(1532..2089)
                     /locus_tag="PLP58_11705"
     CDS             complement(1532..2089)
                     /locus_tag="PLP58_11705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009961353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HRJ09229.1"
                     /translation="MADTTAPELMTVKETAEYLRIPLPTVYYLVQRGQLPAVQIGGRW
                     RIKRSLLDRDVLRKEDEAGQPTVMVVDDDPALQALFKQFLKKAGFGRLVVGTGAEAIS
                     VAKKQRFDFVFLDLKLPDVPGDEVYTQLKELHPDLPIVVITGYPDSEILSRILSHGPV
                     TVIKKPLEYDQINKAVKQLGHKGAE"
     gene            2415..3395
                     /locus_tag="PLP58_11710"
     CDS             2415..3395
                     /locus_tag="PLP58_11710"
                     /inference="COORDINATES: protein motif:HMM:NF013530.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HRJ09230.1"
                     /translation="MKVIITGGGGFLGSQLCEKILERGSLSGPSGGQEPVDEIVLFDA
                     RFHLASSEPRVRQVCGDISDRDTVREVVGARADTAVFHLASMVSGECEERFDDALRVN
                     LDGGRHLFEAARAAAGRPRVVFASSIACFGGEGMEPVNTDRTKHTPQTTYGMTKAICE
                     MLVNDHTRKGHFDGRSVRLPTVIIRPGRPNAAASSWASGMFREPLKGEACHLPVRRDQ
                     CHPMTGYRTVVESFIALHEAPEMRLGSDRAYVLPAHRVTPELADRVLREVAERQKLAL
                     GPIVDAFDPRIQGIVDNWPQAVDGARAVALGMPEPPPLREIVEEYLADFG"
     gene            complement(3405..4814)
                     /locus_tag="PLP58_11715"
     CDS             complement(3405..4814)
                     /locus_tag="PLP58_11715"
                     /inference="COORDINATES: protein motif:HMM:NF014500.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HRJ09231.1"
                     /translation="MHPLLILLIGTLLVIFCIVRLRIGAFFGLMLAAFTVSFIAPGEF
                     ADKLPRVIAALGVAAGKLAVIITCAALIGKCMTESGAADRIVRTFLRMFGERRAPAAL
                     MSSGFVLSMPVFFDTVFYLLVPLARSLHLATKKNYLLYILALAGGAVATHTLVPPTPG
                     PLAAAEALNVDMGMMILMGIVVAVPSALTALALAHWMQGRHTIPMRPLHGEAPPDPEA
                     RLDDSTMLPGFFASCLPIALPVLMMASGTLVSMFAAEAPEGSWAREAAMFMKVAGDPN
                     AALMAAALASVLVYVRQRRPGAARLGREIEESMMSAGTIILITAAGGAFGAMLKEAGV
                     GPAIQKLFSEGSPPAGVMLIVMAWVISALLKTSQGSTTVAMITSSGMMAAMLDAQTLP
                     FHPVYLALAVGFGGLCVVWMNDSGFWIIARMSGLTEIEVLKTWTVLLVIVSITGLVIT
                     ALLAAVLPMPPAPVAPGAG"
     gene            complement(4884..6728)
                     /locus_tag="PLP58_11720"
     CDS             complement(4884..6728)
                     /locus_tag="PLP58_11720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009962821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CocE/NonD family hydrolase"
                     /protein_id="HRJ09232.1"
                     /translation="MIPPPLLLLPALSLFLTALHAQEVSVEKDIMVPMRDGVKLATDV
                     YRPAKDGKAVEGKLPVILTRLPYNKDGSKRFGEYFAKNGYVFVSQDCRGRYKSEGVWH
                     WMSDDGPDGVDCAVWIGRQPWSNGKIGMVGTSYVGGTQHAMALAGAPELTTIIPVDAV
                     SNCGVQSVRNAGAFEMRFWNWIMLNAAKGSRGAQDSGTQAVLQEMTDNRFHYLNNLPL
                     RPGMTPLRLAPEYEEWLVGAMSNGADNEYWKPNNILDHTAQYKDIPVYLVGGWYDSWA
                     GNTTANFAALSKTLKSDVYLIMGPWIHGAQAKSSHGQVDFGPDAAIPDELAWRKQWFD
                     HFMKGEDSGIGKDGHFATKVRIFVMGTGDGKKTGKGLLNHGGSWRSEKEWPPAKSKPT
                     KYHLGKGGALSTDTSTGSTSYTFDPKNPVPTIGGCISSGNDILLQGAWNQVGGPHVWN
                     WPNPVPLSARNDVVVFQTEPLAQDTEVTGEIEVKLWVSSTSVDTDFTAKLVDVYPPSA
                     DWPGGFDMNLTDGIVRARFRDSLKTEKLMERGRIYPVTIRLYPTSNVFKKGHRIRVDI
                     SSSNFPRFDVNPNTGEPLNDNRRVETAVNTIHHDAAHPSHIALPVAGR"
     gene            complement(6776..7432)
                     /locus_tag="PLP58_11725"
     CDS             complement(6776..7432)
                     /locus_tag="PLP58_11725"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HRJ09233.1"
                     /translation="MRGFHTRRLRQEILETLAPDDPAAIKAREELILINHVMGNHRWI
                     AGTLRHHGLAGARLIELGAGDGSLSLKLLNEGLCEARQLHGVDLAPRPGAWPVEAGWT
                     QGDLFAQPLPEGEVMLANLFLHHFEDDRLRELGARLPASARVILAAEPERRRRHALLG
                     RLLCGIAELHPVQHYDMQVSIRAGFCGDELPRALGLGAEWQVLTWGTLFGSYRMIAWR
                     "
     gene            complement(7489..8775)
                     /gene="sufD"
                     /locus_tag="PLP58_11730"
     CDS             complement(7489..8775)
                     /gene="sufD"
                     /locus_tag="PLP58_11730"
                     /inference="COORDINATES: protein motif:HMM:TIGR01981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HRJ09234.1"
                     /translation="MPTTLPNLSPTRDSSTPDWFRARSEAAWTEFQSLPLPGLKDEHW
                     RYSNAKKIELETHAPAKTPTAEQSKAALAATEGLKERAARFVFVNDTLVESDTTGLPA
                     GVVCVSFEEALKSHETALQQHFMKGDLPLGSGKFAALHLAHVKAGTVIIVPKNVVIEK
                     PVEIFHWVVGDHAAIFPHTLVVTGDHAQVSVVDHYRSLEGEGGLSIAIADLVGGSGSQ
                     ITYAACQELADDAQALHLSSITAGRDASVKSFQVQLGAAFSRSESVSDLVGQGARSDM
                     LSVSLPIGDQIVDQRTLQRHKAPHATSDLLYKNALYGQSRSIFSGLIIVDEGAHYTDA
                     YQTCRNLVNSEEAEANSMPGLEINADQVKCSHGATSGPIPDEELFYLKARGIPDQESR
                     KLIVEGFLSDVIERFGNSEVLDTLVARIDEKLERAE"
     gene            complement(8816..9838)
                     /locus_tag="PLP58_11735"
     CDS             complement(8816..9838)
                     /locus_tag="PLP58_11735"
                     /inference="COORDINATES: protein motif:HMM:NF024706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virulence RhuM family protein"
                     /protein_id="HRJ09235.1"
                     /translation="MSHLPSSAEITIFETEDGRTRVEVRFENENVWLTQKLMAELYEC
                     SADNISLHLRNIFADRELDENSVTEESSVTAADGKKYRTKLYSLEAIIAVGYRVQSAR
                     ATQFRTWATDRLKSYILKGFAIDKERFKHGTRFDTRYFDELLEEVREIRASERLAYQK
                     ITDIFATAIDYSPSTAAAEQFFATVQNKLHFAITGRTAAEIIAERADKKLPTMGLTTW
                     RRAPKGKILKSDVGIAKNYLQADELKSLNHIVDMYLDHAEFQASRGRAMRMTDWITRL
                     DAFLQFNEQEILQDKGRVSHAVAMALAEKEYADFRIRQDREFESDFDQLVKQLPKAKT
                     KRPKSE"
     gene            complement(9835..10335)
                     /locus_tag="PLP58_11740"
     CDS             complement(9835..10335)
                     /locus_tag="PLP58_11740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ09236.1"
                     /translation="MLDTVHHADQEWLTKSTVWRSGGMQGDFEVKEGRLWLLLWDVDD
                     NDQQVQLPPEDFQWHGVLELFRNLPLNEGGGRHRAEHRRLIFVDGQLLSETGSVSDVE
                     HDSHWHGDDLPTDPAQRFAWQLQTARGMLSSILANSHSVPTAAIPALKQAETSIAEAL
                     AHLEAQ"
     gene            complement(10360..10869)
                     /locus_tag="PLP58_11745"
     CDS             complement(10360..10869)
                     /locus_tag="PLP58_11745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ09237.1"
                     /translation="MAAPSSLATPELWNSLNWQTVDLVPHTASTKPIVALRCPQDAGL
                     YRITLIDSKWWALASSDVPIKASKKIADGRLESAFWNVPVVLTIGRTTSLKGRLEQHF
                     GTNDKNNRVMKRLSAAFPQLTENQIRESAVSGLLVEVCRIEDWTSRFLLERYGCSLLK
                     PFLDFEAEH"
     gene            complement(10889..12337)
                     /gene="sufB"
                     /locus_tag="PLP58_11750"
     CDS             complement(10889..12337)
                     /gene="sufB"
                     /locus_tag="PLP58_11750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009959275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HRJ09238.1"
                     /translation="MVSSPETTLELPAAVENDISDIRVDSVGDFTFPERNKFDSGFGI
                     TEKTIDYIADVKGDPDWIREFRHKALKVFQEKPMPTHWATKDLENIKFDEFRYYLSDG
                     QKPKRSWDEVPEDVKKTFDRLGIPEQERKFLAGVEAQYDSEAAYSNIKAAVEEQGVIF
                     VNSTEGLHKYPEIFKKWFGKVIPTGDNKFSALNSAVFSGGSFIYVPKGVKVKHPLQAY
                     FRINSEQFGQFERTLIIADEGAEVMYMEGCTAPKFETATLHSAVVELVAMKGAKIQYV
                     TVQNWSSNVFNLVTKRGIAHEDAEVRWIDCNIGSRLTMKYPGVIMKGKRARGEVISIA
                     LANSGQHQDTGAKMVHAADETTSNVISKSISIGEGRATYRGLVHIPKHLKGCKNNTEC
                     DALLINPNSRTDTYPAISVRGNQHATQHEASVSQVSADQIFYLMQRGLTEAEAMSLSV
                     NGFINDLVKEFPMEYSVELKRLIDLEMEGSVG"
     gene            complement(12374..12613)
                     /locus_tag="PLP58_11755"
     CDS             complement(12374..12613)
                     /locus_tag="PLP58_11755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ09239.1"
                     /translation="MTTDPIVAEVRANREAIFAECGYDLDRFNAYMREVSERLKSEGW
                     VFADRPIVRRKPVSYEAAQPESSIVREDPPASTER"
     gene            complement(12615..13082)
                     /locus_tag="PLP58_11760"
     CDS             complement(12615..13082)
                     /locus_tag="PLP58_11760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HRJ09240.1"
                     /translation="MKPSVYIETTIPSYLVARPSRDVVLAGHQQVTQDWWQQSRPNHD
                     LVISQYVIDEVSRGEHEMAQKRLSVLQGIPLLRPSMETETLARELIHRLNLPEQAKTD
                     AFHIAIAAVHGIDFLLTWNCTHINNMHMQRRIEVACREAGFTPPVIGTPEELS"
     gene            complement(13099..13857)
                     /gene="sufC"
                     /locus_tag="PLP58_11765"
     CDS             complement(13099..13857)
                     /gene="sufC"
                     /locus_tag="PLP58_11765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018970778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HRJ09241.1"
                     /translation="MSLEIKNLHAQIPGREILKGLTLTINPGEVHAIMGPNGSGKSTL
                     SKVLCGHEDYEVTSGEVLLDGENILDMAVDARSRAGLFLAFQYPHEIPGVSNANFLRA
                     ALKARLPKGEDIDAVQFYKKLYARMDALEMDRSFTSRSVNEGFSGGEKKRNEILQLMM
                     LEPKYAILDETDSGLDIDALKIVSRGVNAMRSENRGFLVITHYQRLLNYIQPDIVHVL
                     KDGRIVKTGGKELSLELEERGYDWVTEEPLAAAA"
     gene            complement(13891..14343)
                     /locus_tag="PLP58_11770"
     CDS             complement(13891..14343)
                     /locus_tag="PLP58_11770"
                     /inference="COORDINATES: protein motif:HMM:NF013629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HRJ09242.1"
                     /translation="MPPSTSRSIPKPTGLECRMAVLGTDVRLTPHRREVFEVLQGTND
                     HPTATDIFDRVKTRSPGISLATVYNCLDHLTSAGLIKQVHLERGQSRYCANLHEHVHF
                     HCETCGKVIDAHPAAEFDPAKFWNLPKGTKIIRMDIAIHGTCEACASK"
     gene            complement(14525..15811)
                     /gene="hemL"
                     /locus_tag="PLP58_11775"
     CDS             complement(14525..15811)
                     /gene="hemL"
                     /locus_tag="PLP58_11775"
                     /EC_number="5.4.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009965625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0042286 - glutamate-1-semialdehyde
                     2,1-aminomutase activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-1-semialdehyde 2,1-aminomutase"
                     /protein_id="HRJ09243.1"
                     /translation="MPSGPISTKLFELAKNHIPGGVNSPVRAFRNVGGDPFFVRRAKG
                     CRIWDVDGREMIDFVGTWGPAILGHAPVPVIEAIHSAAKEGVSFGIPNPYEVEMARTI
                     CEWVPSIEKVRMVSSGTEATMSAIRLARGFTRRHRLVKFDGCYHGHSDSLLVAAGSGA
                     LTHGHPDSAGVPPAFAELTTVLPFNDEAALEEFFDRQGHETAALIVEPYPANAGLILP
                     KPGFLQKLRDITAKHGVILIFDEVMTGFRLAKGGVQEIEGIKPDLTCLGKVIGGGLPV
                     GAFGGRADIMDHLAPLGPVYQAGTLSGNPVALAAGLAQLRELERQNGYAALEDLGRMM
                     EDAVLGILRSKGLNYRWYRRGSMFCLFFTEKEVGNLQDAKTSDLAAFRRFFHHCLDNG
                     VYFAPSQFETGFISMAHTEADMTRTAEIAAAALAAL"
     gene            15894..16613
                     /locus_tag="PLP58_11780"
     CDS             15894..16613
                     /locus_tag="PLP58_11780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ09244.1"
                     /translation="MKTLTYLFLVLLLPGAAAEAQFSKQRKSMIPPEPGTINVEDMLP
                     KPVMLKVLQEAPVYTRSALEQAIGSMAPGTVVKLIGLSDTAYRVRGRARHGDVSGWVR
                     MGDLLSPDPNLVTNLRKLHERQTQVEEMIANKQVALGMTGDEVQASLGKPARKSTKIN
                     AGGREEKLEYVVYERVPQFTTGLDPLGRPVQSVVYLKVETGNMAVTLKDNVVDSIEET
                     KGNPLGSGGVKIVPGPIFFNW"
     gene            16677..>17061
                     /gene="queF"
                     /locus_tag="PLP58_11785"
     CDS             16677..>17061
                     /gene="queF"
                     /locus_tag="PLP58_11785"
                     /EC_number="1.7.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012420914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033739 - preQ1 synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preQ(1) synthase"
                     /protein_id="HRJ09245.1"
                     /translation="MSDLNELSLLGRSEHRLPASPDEAELETFPNRTPGRNYTIHLTA
                     PDFSSLCPVTGQPDAAHVEIIYIPDERCVETKSLKFYLASYRNHAAFNEAIVNRILDD
                     LVKACAPRQARVRGRFAPRGGIQLTC"
BASE COUNT         3114 a         4775 c         5666 g         3506 t
ORIGIN      
        1 agtctgcggg gccgccgcgc ccgcccggtg tgtcgctggg cgcgcccttc atcgggctgc
       61 tggacaagga ggggaatttc atcagcagtt cgcgcggccg gcaggggaat ccgctggtgg
      121 agccgcggac ggggaacgcg ggcgtggagg gggaggagat ggagctgcgg cgcaggtcgc
      181 tgaaaatgcc gtggctgggc gggcgggatt ttgagagcgt gctgaggaag caggaggcgg
      241 gttacctgat cctggaggcg ggcgggaggc cgcaggtgcg ggaggtgttc atcacgccgc
      301 tgcgctcgcc ggaggatggc agtctgctgg gtgcgtttgc ttttggcctg ccgctgccgg
      361 cgctggcgga gcggctgctg tatgaccaga cgcgccgcgg ggacaatggc gagatcatga
      421 gcggtgtgta tttggagggg cggctggttt cctcaacgct gccggaggcg cgccggactg
      481 tggtggaggg gctgctggag gccgctctgg cgggggcggg tgacgccggg agcgagatca
      541 tcacgctcat cggggaggtg cgtcacaggc tgatctaccg ggtgctcaat cctgacagtc
      601 cttttcccaa ggcggtgcag gtgagctttt atccgctgtc cgcgctggat gaggaaatca
      661 ggggtctgag aaaagggacg gcgggtctgg cgctggcggc gctgctggtt tcgctggggg
      721 tggtgctggc ggtctcgcgc gggctgtccg gtccggtgaa caggctggtg gcggcggtgc
      781 atgagatcga gcaggggaac ttcgagatgc gtgtgctggt gacgcctgat gaaacgggcc
      841 ggctggcgcg cgcgctgaat cagatggcgg cgggcctggc gctgcaggag aagtaccgca
      901 gcgtgcttaa tgcggtggcg gaccgggcgg tggcggagcg gctgattgag aaccgcgagg
      961 cgttgagcgg cgagctgagg agtgtttcgg tgctgttttg cgacatccgc ggcttcaccg
     1021 cgctgagcga gcggatgccg ccgcaggagt tgatcgtgct gctgaacacg cacatgacga
     1081 cgatgacgcg cctggcctac gagcatggcg gcatcgtgga caagtttgtc ggcgatttga
     1141 tcatggtgct gtttggcgcg ccggtgagca cgggccgcga cgcggagcag gcggtggctt
     1201 gcgcgcgggc catgctggcg gagaggcggc ggatgaatga gtcaggggag gggatgccgc
     1261 tggaggtggg gatcggcatt gccacggggg atgtggtggc gggctgcatg gggtctgacc
     1321 agcggctgag ctataccgtg ctggggcacc gggtgaatct ggcctcgcgg ttgtgcagcg
     1381 tggccgggcc gggggaaatc atgacggatg cggagacgat gcgcgcgctg ggcgaaggcg
     1441 cgcgggtgga ggccatgccg ccgatgcggt tgaagggcat gtcggatccg gtggaggtgt
     1501 tccggcttgg ctgagagcgc tcctggcggg atcactccgc gcctttgtgg ccgagctgct
     1561 tgaccgcctt gttgatctgg tcgtattcca gaggcttctt gatcacggtg actggaccgt
     1621 gcgagaggat gcggctgagg atttcgctgt cgggatagcc ggtgatgacc acgatcggga
     1681 gatcgggatg cagttctttg agctgggtgt aaacctcgtc acccggcaca tccggcagct
     1741 tgaggtcgag gaagacgaag tcaaagcgct gctttttcgc cacgctgatg gcctcggctc
     1801 cggtgccgac cacgaggcgt ccgaatccgg cctttttgag gaactgcttg aacaatgcct
     1861 gaagcgccgg gtcatcgtca acgaccatga ccgtaggctg gccggcctcg tcttccttgc
     1921 gcagcacatc gcggtccaaa aggctgcgtt tgatgcgcca gcgcccgcca atttgcacag
     1981 cggggagctg cccgcgttgg acgagataat agacggtggg caaagggatg cggaggtatt
     2041 ccgccgtctc tttgacagtc atcagttcgg gagctgtggt gtcggccatg agggtgaagt
     2101 tctataaatt ataagtcgcc ctgaaattgc gggaatggtt gcgagtctca gaattgattc
     2161 aacgctggta tataattacc attattgcaa gccgcttttt gatattttat ggcaagacgt
     2221 tgtttcataa taaaggacat tgcttcaata cgttctcaga aatgagatat tttgcatgaa
     2281 aagccagttt atttagaata tctaaatatt cctcgatctt aaaataatga atttctagat
     2341 ttgaagtcgt cgcagcttca cacccttgaa ttttgcgctt tcacggcctc gcttgaggcg
     2401 gtacatccgg ttttatgaaa gtcatcatca ccggcggcgg cggatttctt gggtcccagc
     2461 tttgtgaaaa aatccttgag cgcggctcgc tttccggtcc gtctggcggg caggagcctg
     2521 tggatgagat cgtgctgttt gacgcccgct ttcatctggc cagcagcgaa ccgcgcgtcc
     2581 gccaggtctg cggtgacatc agtgaccgcg acacggttcg agaagtcgtc ggcgcgcgcg
     2641 cggatacggc ggtgttccac ctggcctcca tggtaagcgg cgaatgcgag gagcgcttcg
     2701 atgacgcgct gcgggtgaac ctcgacggtg gacgccatct ttttgaggcc gcgcgggccg
     2761 ccgctggaag accgcgcgtg gtctttgcca gcagcatcgc atgtttcggg ggtgagggaa
     2821 tggagcctgt gaacaccgac cgcacaaaac acacgccgca gaccacatac ggcatgacca
     2881 aggccatctg cgagatgctg gtcaacgacc acacccgcaa agggcacttt gacggacgca
     2941 gcgtcaggct gcccacggtc atcatccggc ccggaaggcc aaacgcggcg gcctcgagct
     3001 gggccagcgg catgtttcgc gagccactga aaggcgaggc atgccatctg ccggtgcgcc
     3061 gggatcaatg ccatccgatg acgggctacc gcacggtggt ggagtcattc atcgccttgc
     3121 atgaggcgcc ggaaatgcgg cttggcagcg accgcgctta tgtgctgccc gcgcatcggg
     3181 tgacacctga actggcggac agggtgctgc gcgaggtggc tgagaggcaa aagctggcgc
     3241 tcggccccat tgtggacgct tttgatcccc gcatccaggg catcgtggac aactggccgc
     3301 aggccgtgga cggggcgcgc gctgtcgctc tgggcatgcc tgagccgcct ccgctcaggg
     3361 agatcgtcga ggagtatttg gccgactttg gatgaaaatc ctggtcagcc cgctcccggc
     3421 gcgacgggag ccggcggcat cggcagcaca gcggccagca gcgccgtgat gaccagcccg
     3481 gtgatgctga caatgacaag cagcactgtc caggttttga gcacttcgat ttcggtcagg
     3541 ccgctcatgc gcgcgatgat ccaaaagccg ctgtcattca tccagactac gcacagcccg
     3601 ccaaagccca ccgccagcgc cagataaacc gggtgaaacg ggagagtctg cgcgtcgagc
     3661 atcgccgcca tcatgccgga gctggtgatc atggccacgg tggtggaacc ctggctggtc
     3721 ttcagcagcg ccgaaatcac ccaggccatg acaatgagca tcactccggc aggggggctg
     3781 ccttcggaga acagcttctg gatggccggc cccacgcccg cctccttcag catggctccg
     3841 aaagctccgc ccgccgccgt gatgaggatg atggtgcccg cgctcatcat ggactcctcg
     3901 atttccctgc caagccgcgc cgctcccggt ctgcgctgcc tcacataaac caggactgac
     3961 gccagcgcgg cagccatgag cgcggcattt gggtcacccg ccaccttcat gaacatggcc
     4021 gcctcacgcg cccaggagcc ctctggagcc tcggcagcaa acatggacac aagggtgccg
     4081 gaagccatca tgagcaccgg cagcgcgatc ggcaggcatg aggcaaaaaa acccggcagc
     4141 atcgtggaat catccagtct cgcttccggg tccggcggcg cctctccatg cagggggcgc
     4201 atggggatgg tgtggcggcc ctgcatccag tgcgccagcg ccagcgcggt caaggcgctc
     4261 ggcacagcga ccacgatgcc catcaggatc atcatgccca tgtccacatt gagggcctcc
     4321 gcggccgcca gcgggcccgg cgtcggcggc accagcgtgt gcgtggccac cgcgccgccc
     4381 gccagcgcca ggatgtagag caggtagttc tttttcgtgg ccaggtggag cgagcgggcc
     4441 agcggcacca aaagataaaa caccgtgtca aaaaaaaccg gcatagacag cacaaagccg
     4501 gaggacatca atgccgccgg agcgcgccgc tcgccgaaca tccgcagaaa ggtgcgtacg
     4561 atgcggtccg ccgcgccgct ctccgtcatg catttgccga tgagcgcggc gcaggtgatg
     4621 atgacagcca gctttcccgc ggccaccccc agggcggcga tcacgcgcgg cagcttgtcg
     4681 gcgaactcgc ccggagcgat aaaactgacc gtgaacgcgg ccagcatcag cccgaaaaac
     4741 gcgccgatgc gcaggcgcac gatgcagaaa atgaccagca aagtgccgat caggagaatc
     4801 aaaagcggat gcatgaagcg cgcaggctac ggggatccgg aaatcttggc gatgctaaaa
     4861 tgcgggcatt ccctgccgct tcatcatctt cccgccactg gcagcgcgat gtgggagggg
     4921 tgggccgcgt cgtggtggat ggtgttcacg gcggtctcga cgcggcggtt gtcgttgagc
     4981 ggctcgcccg tgttgggatt cacatcgaag cgcgggaaat tgctgctgct gatgtccacg
     5041 cggatgcggt ggcctttttt gaagacgttg ctggtgggat aaagacggat cgtcaccgga
     5101 tagatgcgcc cgcgctccat gagcttctcg gtcttgagcg agtcgcggaa gcgcgcgcgc
     5161 acgatgccat cggtgaggtt catgtcgaag ccgcccggcc agtcagccga gggcggatac
     5221 acgtccacca gcttcgcggt gaagtcggtg tccacggaag tggaggaaac ccagagcttc
     5281 acttcgatct cgcccgtgac ctcggtgtct tgcgccagag gttccgtctg aaaaacgacc
     5341 acgtcattgc gcgcggagag cggcacgggg ttcggccagt tccacacatg cgggccgccc
     5401 acttgattcc acgcgccctg gagcaggatg tcattgccgc tggagatgca gccgccaatg
     5461 gtgggcacag ggttcttcgg atcaaaggtg tagctcgtgc tgccggtgct cgtgtccgtg
     5521 gagagcgcgc cgcctttgcc gagatgatac ttcgtgggct tggacttcgc gggcggccat
     5581 tccttttcac tgcgccagga gccgccgtgg ttcagcaggc ccttgccggt ctttttgcca
     5641 tcacccgtgc ccatgacaaa gatgcgcacc ttggtggcaa aatggccgtc tttgccgatg
     5701 ccgctgtcct cacccttcat gaagtggtcg aaccattgct tgcgccaggc cagctcgtcc
     5761 gggatggccg catcggggcc gaagtccacc tgcccgtggc tgctcttcgc ctgcgcgccg
     5821 tggatccacg gtcccatgat gagatagaca tcgcttttca gcgtcttcga caacgcggca
     5881 aaattcgccg tcgtattgcc cgcccaggag tcataccagc cgccgacgag atagaccggg
     5941 atgtctttgt actgcgccgt gtggtcgagg atgttgttcg gcttccaata ctcattgtcc
     6001 gcgccattgc tcatggcgcc gacgagccac tcctcatact ccggggccag ccgcagcggc
     6061 gtcataccgg ggcgcagggg aaggttgttg aggtaatgaa agcggttgtc cgtcatctcc
     6121 tggagcaccg cctgagtgcc tgagtcctgc gcgccgcggc tgcccttggc ggcattgagc
     6181 atgatccagt tccagaagcg catctcaaac gcgcccgcgt tgcgcacgga ctgcacgccg
     6241 cagttagaca cggcatccac tggaatgatc gtcgtcagtt ctggcgcgcc tgccagcgcc
     6301 atggcgtgct gcgtgccacc cacataggaa gtgccaacca tgccgatttt gccattgctc
     6361 cacggctgcc tgccaatcca gacggcgcaa tccactccgt cagggccgtc atcgctcatc
     6421 cagtgccaca cgccctcgct tttgtagcgg ccccggcagt cctgcgagac aaagacgtaa
     6481 ccgtttttgg caaagtattc accgaagcgc ttcgagccgt ccttgttgta gggcagacgg
     6541 gtcaagatga ccggcagctt tccctccact gctttgccgt ccttcgctgg gcggtaaaca
     6601 tccgtggcca gcttcacgcc atcccgcatc ggcaccatga tgtctttttc caccgatacc
     6661 tcctgggcgt ggagcgcggt caggaaaagg gaaagcgcgg gcagcagcaa tagaggggga
     6721 gggatcatga ggcggagttt aacgggcagc gcgcgcacat gcttgcccgc aaagctcagc
     6781 gccaggcgat cattcggtat gagccaaaca aggtgcccca agtgagcacc tgccactccg
     6841 cgccaagacc aagcgcgcgc ggcagctcgt ctccgcagaa tcccgcgcgg atgctcacct
     6901 gcatgtcgta atgctgaacc ggatgcagct cggcgatgcc gcagagcagc cgaccaagca
     6961 gcgcgtgacg gcggcgtctt tccggctcgg cggcgaggat gacacgcgcc gaggcgggaa
     7021 gacgcgcgcc caactcgcgc aggcggtcgt cttcgaaatg atgcagaaac aagttcgcaa
     7081 gcatcacctc gccctcgggc aggggctgcg caaacagatc cccctgcgtc cagcccgcct
     7141 caaccggcca cgcaccgggc cgtggagcga ggtccactcc gtgcaactgc cgtgcttcgc
     7201 aaagcccctc gttcaaaagc ttcaacgaaa gcgagccgtc acccgcgccc agctcgatca
     7261 aacgcgcgcc tgccagcccg tgatggcgca gtgtgcctgc gatccacctg tgattgccca
     7321 tcacatgatt gatgaggatt agttcctccc tggccttgat ggcggcggga tcgtcaggag
     7381 caagcgtctc caaaatttcc tgccgcaacc tccgggtgtg gaagccccgc atttgccaaa
     7441 atggaaaaac ggggaaacgc gacggccgat ttgaagggca gtcacccatc actcggcgcg
     7501 ctccagcttt tcatcaatgc gcgccacgag cgtgtcgagc acctcgctgt tgccgaagcg
     7561 ctcaatcaca tccgagagga agccctcgac aatcagcttg cgcgattcct ggtcaggaat
     7621 gccgcgtgct ttgagataaa aaagctcctc gtcaggaatc ggcccgctcg tcgcgccatg
     7681 gctgcatttg acctggtcgg cgttgatctc gagaccgggc atgctgttgg cttcggcttc
     7741 ctcactgtta acgaggttgc ggcaggtttg ataagcgtca gtgtaatgcg cgccttcatc
     7801 cacgatgatg aggccgctaa agatgctgcg ggactggccg tagagggcgt ttttgtagag
     7861 caggtcgctg gtggcgtgcg gggctttgtg acgctgaagc gtgcgctgat cgacgatttg
     7921 atcgccgatg ggcaaactga cgctgagcat gtcgctgcgg gcgccctggc cgacgagatc
     7981 gctcacgctc tcgctgcggc tgaaggcggc gccgagctgc acctggaagc ttttcacgct
     8041 ggcgtcacgt ccggcggtga tgctggaaag atgcagcgcc tgggcgtcat cggccagctc
     8101 ctggcaggcg gcgtaggtga tctggctgcc gctgccgccg acgagatcgg cgatggcgat
     8161 gctgaggccg ccttcgcctt cgagactgcg ataatgatcg acgacgctga cctgcgcgtg
     8221 gtcgccggtg acgacgagcg tgtgtgggaa gatggccgcg tggtcgccaa cgacccagtg
     8281 gaagatttca acgggctttt cgatgacgac attcttcggc acgatgatga cggtgcctgc
     8341 tttgacatgc gcaaggtgca gcgccgcgaa tttgccggac ccgagcggca gatcgccctt
     8401 catgaagtgc tgctggaggg cggtttcgtg cgatttgagg gcttcctcga agctcacgca
     8461 cacgacaccg gcgggaaggc cggtcgtgtc ggactcgacg agcgtgtcat tcacgaagac
     8521 aaagcgtgcc gcgcgttctt tgagaccttc cgtggctgcg agcgcggctt tcgactgctc
     8581 ggcggtcggt gttttggccg gagcatgcgt ttcgagctcg atcttcttcg cgttcgagta
     8641 gcgccagtgt tcgtctttga ggccgggaag cgggagggac tggaattcgg tccaagcggc
     8701 ttctgagcga gcgcggaacc agtcgggcgt ggaggaatcg cgggtgggag agaggttggg
     8761 gagagtggtg ggcatgtttg aaaatgacga atggcgaatt cagaatgacg aagggtcact
     8821 ctgacttggg acgcttggtt ttggctttgg ggagttgttt gacgagttga tcaaagtcgg
     8881 actcgaactc gcggtcctgg cggatgcgaa agtcggcgta ttctttttca gcaagcgcca
     8941 ttgcaacagc atgcgacacg cgacctttgt cctggaggat ttcttgctcg ttgaattgga
     9001 ggaaggcatc tagccgggtg atccagtccg tcatgcgcat ggcacgtccg cggctggcct
     9061 ggaactcggc gtggtcgagg tacatgtcca cgatgtggtt cagcgacttg agttcgtctg
     9121 cctggagata gttcttggcg atgccgacgt ccgacttgag gatcttgccc ttgggagcac
     9181 gcctccaggt ggtgaggccc atggtcggga gttttttgtc cgcacgctcg gcgataatct
     9241 ctgctgccgt gcggcctgtg atggcaaaat ggagcttgtt ctgcaccgtg gcgaaaaact
     9301 gctctgcagc ggcggtgctg ggcgagtagt cgatggccgt ggcgaagatg tcggtgatct
     9361 tttggtaggc gaggcgctca ctggcgcgga tttcccgcac ctcctccagc aattcgtcaa
     9421 agtagcgagt gtcgaagcgg gtgccgtgct tgaaccgctc cttgtcgatg gcgaagcctt
     9481 tgaggatgta gcttttaagc cggtccgtgg cccaggtgcg gaattgtgtc gcccgggcgc
     9541 tctgcacacg gtatccgaca gcgatgatgg cttcgaggct gtagagcttg gtccggtatt
     9601 ttttgccatc ggcggcagtt accgaggatt cctcggtaac tgaattctca tcgagttccc
     9661 ggtctgcgaa gatgttccgc aggtgcaggg atatgttgtc agcgctgcac tcatacagct
     9721 ccgccatgag tttttgggta agccagacgt tttcattctc aaaccgcacc tccacacgag
     9781 tccttccatc ttcggtctcg aagatggtga tctcagcaga tgacgggaga tgactcattg
     9841 ggcttctaaa tgggctagag cctcagcaat cgaggtttca gcctgcttga gagcaggaat
     9901 ggctgctgtt ggcactgaat gggagtttgc tagaatgctg gagagcatcc cgcgtgcagt
     9961 ttgaagctgc caggcgaagc gctgggcggg atccgtcggc aaatcgtcgc catgccagtg
    10021 cgaatcgtgc tccacatcgg agacggagcc ggtttcgctc aagagctgtc catctacgaa
    10081 aatgaggcgg cgatgctcgg ctcgatgtct tcctccgcct tcgttcaggg ggagattgcg
    10141 gaagagttcg aggacaccat gccactgaaa gtcttcagga ggcagttgga cttgttgatc
    10201 gttatcatcg acgtcccaaa gcaagagcca caagcgcccc tctttgacct caaaatcgcc
    10261 ctgcatgccg cctgagcgcc aaacagtgct tttggtgagc cattcctggt ctgcgtggtg
    10321 aacggtgtcg agcatgctca taacgatggc tggctagttt tagtgctcag cttcaaagtc
    10381 taagaagggc ttgagcaacg aacaaccgta acgttcgagt agaaaacggc tggtccaatc
    10441 ttcaatgcgg cacacttcga caagaaggcc gcttacagca gactcgcgaa tttgattttc
    10501 tgttagctgg ggaaaggctg cggaaagccg cttcatgact ctattgtttt tgtcattagt
    10561 cccaaaatgt tgctcaaggc ggccttttag cgaggtcgtt cgcccgatgg tcagcacgac
    10621 tggcacattc caaaaagcgc tttccaaacg cccatcggca attttttttg aagctttgat
    10681 tggcacgtcg gatgaagcta gtgcccacca tttgctgtcg attaatgtaa ttcggtaaag
    10741 acctgcatct tgggggcacc ttaaagcaac aatcggtttt gttgatgctg tgtgaggaac
    10801 caaatcgact gtttgccaat taagtgaatt ccacaattca ggcgtagcta ggcttgaagg
    10861 tgctgccatg tgctttgagt gagacttttt aaccgacgct tccctccatc tccaggtcga
    10921 tgagcctttt caactccacg ctgtattcca tggggaattc cttcacgagg tcgttgatga
    10981 agccgttgac gctgaggctc atggcttcgg cttcggtgag gccgcgttgc atgaggtaga
    11041 agatttggtc ggcgctgacc tggctgacgc tggcttcgtg ctgggtggca tgctggttgc
    11101 cgcggacgct gatggcgggg taggtgtcgg tgcggctgtt ggggttgatg agcagggcgt
    11161 cgcactcggt gttgtttttg cagcctttga gatgctttgg gatgtgcacg aggccgcggt
    11221 aggtggcgcg gccttcgccg atgctgatgg atttgctgat gacgttgctg gtggtctcgt
    11281 cggcggcgtg gaccatcttc gcgccggtgt cctgatgctg gccgctgttc gccaaggcga
    11341 tgctgatgac ctcgccacgg gcgcgtttgc ccttcatgat gacgccgggg tatttcatgg
    11401 tgaggcggct gccgatgttg cagtcgatcc agcgcacttc ggcgtcttcg tgggcgatgc
    11461 cgcgcttggt gacgaggttg aagacgttgc tgctccagtt ttgcacggtg acatactgga
    11521 tcttcgcgcc tttcatggcg acgagttcga cgacggcgct gtgcagcgtg gcggtctcaa
    11581 atttcggcgc ggtgcagcct tccatgtaca tcacttccgc gccttcatcg gcgatgatga
    11641 gcgtgcgctc gaactggccg aactgctcgc tgttgatgcg gaaataggcc tgcagcggat
    11701 gcttcacctt cacgcctttc ggcacgtaga tgaacgaacc gccggagaac acggcgctgt
    11761 tgagcgcgga gaatttgttg tcgccggtgg ggatgacctt gccgaaccac ttcttgaaga
    11821 tctcggggta tttgtgcagg ccctcggtgc tgttgacgaa gatgacgccc tgctcctcga
    11881 cggcggcctt gatgttcgag taggcggcct cggagtcata ttgcgcctcg acgccggcga
    11941 ggaatttgcg ttcctgctcg gggatgccga gtcggtcgaa ggtcttcttc acgtcctcgg
    12001 gcacctcgtc ccaggagcgc ttcggcttct ggccgtcgct gaggtagtag cggaactcgt
    12061 cgaacttgat gttttcgagg tccttcgtgg cccaatgcgt gggcatgggc ttttcctgga
    12121 agaccttcaa ggctttgtgc cggaactcgc ggatccaatc gggatcgccc ttcacatcgg
    12181 cgatgtagtc gatggtcttt tcggtgatgc cgaagccgga gtcgaacttg ttgcgctccg
    12241 ggaaggtgaa gtcgccgacg ctatcgacgc ggatgtcgga gatgtcgttt tcgacggcgg
    12301 ctggaagttc gagggtggtt tctggggagg agaccataaa agtgaaaggt tagaggtgag
    12361 aagtcagatg tgattagcgc tcggtggagg ctggtggatc ttcgcggacg atggaggatt
    12421 caggctgtgc ggcctcataa gagacaggct tgcgacgcac gatggggcgg tcggcgaaga
    12481 cccagccttc gcttttgagc cgctcgctca cctcgcgcat gtaggcgttg aagcggtcga
    12541 ggtcatagcc gcattccgcg aagatggctt cgcggttggc gcggacttcg gcgacgatgg
    12601 gatcggtagt catgtcagct gagttcctcc ggggttccaa tcacaggcgg cgtgaaaccc
    12661 gcctcgcggc aggcgacctc gatacgccgc tgcatgtgca tgttgttgat gtgggtgcag
    12721 ttccaggtca gcaagaagtc gatgccatga acggcggcga tggcgatatg aaacgcatcg
    12781 gtcttcgcct gttcgggaag gttcagccga tggatcagtt ctcgcgccaa agtttccgtt
    12841 tccatgcttg gacgcagtag cgggatgccc tggagcacac tcaaacgctt ctgcgccatc
    12901 tcgtgttctc cgcgcgagac ttcgtcgata acgtattgcg agatgaccaa atcatgattc
    12961 ggccgcgact gctgccacca atcctgagtg acctgctgat gccccgcgag caccacatca
    13021 cgactcggcc gcgctacaag gtagctcggg atggtcgttt cgatgtacac ggagggtttc
    13081 atgagtgtga tcggtgattt atgcagcagc ggccaaaggc tcctcggtga cccagtcgta
    13141 gcctcgttct tcgagttcga gggagagctc tttgccgccg gttttgacga tgcggccgtc
    13201 tttgaggacg tggacgatgt cgggttggat gtagttcagc agacgctggt agtgtgtgat
    13261 gacgaggaag ccgcggttct cgctgcgcat ggcgttcacg ccgcgggaga cgatcttgag
    13321 ggcgtcgatg tcgaggccgg agtcggtttc gtcgaggatg gcgtatttcg gctccagcat
    13381 catgagctgg aggatttcgt tgcgcttctt ttcaccgccg gagaagcctt cattgacgct
    13441 gcggctggtg aaactgcggt ccatctccag ggcgtccatg cgggcgtaga gctttttata
    13501 gaattgcacg gcgtcaatgt cctccccctt cggcaggcgg gccttcagcg cggcgcggag
    13561 gaagttcgcg ttgctgacgc cggggatctc gtgcggatac tggaaggcca aaaagagccc
    13621 ggcgcggctg cgggcatcga cggccatgtc gaggatgttt tcgccatcga ggaggacctc
    13681 gccgctggtg acttcgtagt cttcgtggcc gcagaggact ttgctcagcg tgcttttgcc
    13741 ggagccgttc ggacccatga tcgcgtggac ttcaccggga ttgatcgtga gggtgaggcc
    13801 tttgaggatt tcgcggccgg ggatctgggc gtggaggttt ttgatttcga gggacatgaa
    13861 aattcggaat ggagaatgcg gattgcggaa ttacttggat gcgcaggctt cgcaggtgcc
    13921 gtggatggcg atgtccatgc ggatgatttt ggtgcctttg ggcaggttcc aaaatttggc
    13981 ggggtcgaac tcagcggcgg ggtgggcgtc tatgaccttg ccgcaggttt cgcagtggaa
    14041 atggacgtgc tcgtggaggt tcgcgcagta gcgggactgg ccgcgttcca ggtggacctg
    14101 cttgatgagc ccggcggacg tgaggtggtc gaggcagttg taaacggtag ccagggagat
    14161 gccgggggag cgggttttca cccggtcaaa gatgtccgtg gcggtcgggt gatcgttggt
    14221 tccctggagc acttcaaaaa cttcccgtct gtgcggggtg aggcgtacat cggtgccgag
    14281 cacggccatc cggcattcca ggccggtagg cttggggatg gaacgagaag tggaaggagg
    14341 catgagattt tagaattaga atgattctaa aatattgaaa cggacctcat gcaagagcgg
    14401 atgctggttc gcgggaaaaa ttttccgcga gctgcgcttt gaggggcggg cggaaagact
    14461 ggcaggagcg gcaccagaaa cgcaggcatg acatgcccga caaggttcca cccgggcttt
    14521 cggttcaaag cgctgccagt gctgctgccg cgatctcggc agtgcgggtc atgtcggcct
    14581 cggtgtgggc catgctgatg aagccggtct cgaactggct gggtgcgaag tacactccgt
    14641 tgtcgaggca gtggtggaaa aatctgcgga aggcggcgag atccgaggtc ttggcgtcct
    14701 ggagattgcc gacttctttc tccgtaaaaa acaggcagaa catgctgccg cggcggtacc
    14761 agcggtagtt caggcccttg gaccgcagga tgccgagcac ggcgtcttcc atcatgcggc
    14821 cgaggtcttc aagcgcggcg tagccgttct gcctttcgag ttcgcgcagt tgcgcgagcc
    14881 cggcggcgag tgccacggga ttgccgctga gagtgcccgc ctgatacacc gggccgagcg
    14941 gtgcgagatg gtccatgatg tccgcccgcc cgccaaaggc ccccacgggc agaccgccgc
    15001 cgatgacctt gcccaggcag gtgagatccg gcttgatgcc ttcgatttcc tgcacgccgc
    15061 ctttggccag tcggaagccg gtcattactt cgtcaaaaat caaaatgacg ccgtgcttcg
    15121 ccgtgatgtc gcgcagcttt tgcagaaagc ccggcttggg cagaataagg ccggcgtttg
    15181 ccgggtaggg ttcgacgatg agcgcggcgg tttcgtgccc ctgtctgtca aaaaactcct
    15241 ccagcgcggc ctcgtcattg aagggcagca cggtggtcag ctccgcgaag gcgggcggca
    15301 cgccggcgct gtccgggtgg ccgtgcgtga gcgcgccgct gccggctgcg acgagcaggc
    15361 tgtcgctgtg gccgtggtag cagccgtcaa atttcaccag cctgtgcctg cgggtgaatc
    15421 cgcgggcgag gcggatggcg ctcatggtgg cctcggtgcc gctgctgacc atgcgcactt
    15481 tttcaatgct tggcacccat tcgcaaatcg tgcgcgccat ttccacctcg taggggtttg
    15541 gaatgccaaa gctcaccccc tccttggctg cgctgtggat ggcctcaatc acgggcacgg
    15601 gcgcgtggcc gaggatggca ggcccccagg tgccgacgaa gtcaatcatc tcgcgcccgt
    15661 ccacatccca gatgcggcag cctttggcgc ggcggacgaa gaacgggtcg ccgccgacat
    15721 tgcggaaggc gcgcacgggc gagttcacgc cgccgggaat gtgatttttt gcgagttcga
    15781 agagcttggt ggagatgggg ccgctgggca tggcgggcag gcttgcacgg cggcggcgga
    15841 gagtcgaaca aaaggttttc cttgcgcgtc agaatccggt attctcaggt caaatgaaga
    15901 cgctcactta tctttttctg gtgcttttgc tccccggtgc ggccgctgaa gcccagttct
    15961 ccaagcagcg caagtccatg atcccgcccg agccgggcac catcaatgtg gaggacatgc
    16021 tgcccaagcc ggtgatgctc aaggtgctcc aggaggcgcc ggtttacacc cggtcggcgc
    16081 tggagcaggc catcggctcc atggcgccgg gcacggtggt gaagctcatc ggcctgtccg
    16141 acaccgccta ccgcgttcgc gggcgcgcgc gccacggcga tgtctccggc tgggttcgca
    16201 tgggtgatct gctttcaccg gatccgaatc tggtgacaaa cctgaggaag ctccacgagc
    16261 gccagacgca ggtggaggaa atgatcgcca acaagcaggt ggcgctcggc atgactggag
    16321 atgaggtgca ggccagcctt ggcaaacccg cgcgcaagtc caccaagatc aatgccggcg
    16381 gcagggagga gaagctcgaa tacgtcgttt atgagcgcgt gccgcagttc accaccgggc
    16441 tggacccgct cggcaggccg gtgcagagcg tggtgtacct caaagttgaa accggcaaca
    16501 tggccgtaac cctcaaggac aacgtggtgg acagcatcga ggagaccaaa ggcaacccgc
    16561 tcggcagcgg cggagtgaaa atcgtgccgg gaccgatttt cttcaattgg tgagttgaga
    16621 gttgggggcg ggccatcagg cttgcagggc gggcatgtcc cggctacttg gcgggcatgt
    16681 ccgacctcaa cgaactttca cttctgggcc gctccgagca ccgtcttccc gcctcgccgg
    16741 acgaggctga gctggagacg tttccaaacc gaacaccggg ccggaactac accatccatc
    16801 tcaccgcgcc ggacttcagt tcattgtgcc ctgtcacagg ccagccggat gccgcgcatg
    16861 tggagatcat ctacatcccg gacgagcgtt gcgtggagac gaagtcgctg aaattttacc
    16921 tggccagcta ccgcaaccac gccgccttca acgaagccat cgtcaaccgc atcctggacg
    16981 acctggtcaa agcctgcgcg ccgcgccagg cgcgcgtgcg cggacgcttc gccccgcgcg
    17041 gcggcatcca gctcacctgc g
//
LOCUS       DAOMYJ010000101        17046 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Prosthecobacter sp. isolate AMR_MDS_5629
            NODE_1159_length_17046_cov_20.510094, whole genome shotgun
            sequence.
ACCESSION   DAOMYJ010000101 DAOMYJ010000000
VERSION     DAOMYJ010000101.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867850
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prosthecobacter sp. (wastewater metagenome)
  ORGANISM  Prosthecobacter sp.
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Verrucomicrobiaceae; Prosthecobacter.
REFERENCE   1  (bases 1 to 17046)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17046)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.30
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:34:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,742
            CDSs (total)                      :: 4,684
            Genes (coding)                    :: 4,676
            CDSs (with protein)               :: 4,676
            Genes (RNA)                       :: 58
            tRNAs                             :: 55
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17046
                     /organism="Prosthecobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1159_length_17046_cov_20.510094"
                     /isolate="AMR_MDS_5629"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1965333"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            222..2234
                     /gene="acs"
                     /locus_tag="PLP58_11790"
     CDS             222..2234
                     /gene="acs"
                     /locus_tag="PLP58_11790"
                     /EC_number="6.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009965881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003987 - acetate-CoA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0019427 - acetyl-CoA biosynthetic process
                     from acetate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase"
                     /protein_id="HRJ09246.1"
                     /translation="MSQKAVSQKTAKAAKNVKSATTSTKSLLVENRVFKPAAEFSRKA
                     RISSMAHYRKMHEESLRHPEKFFAREAKELAWRKPFKKVLDWKCPNAKWFTGGQLNVS
                     ENCLDRHLDGPRKTKAAIIWEGEPGEKRVITYQQLHRDVCRFSNVLKRHKVRKGDRVV
                     IYMPMVPEAAVAMLACTRIGAVHSVVFGGFSAESIKDRVLDSGATVVITSDGGYRRGA
                     VVPLKKNVDDALKGGDTPVKTVIVLRRTGQDVHIEEGRDYWWHRELEYVDANCPAVAL
                     DSEAPLFILYTSGSTGKPKGILHSTAGYLLHAQMTCKYVFDLRDDDVYWCTADIGWVT
                     GHSYMVYGPLAMGATSLMYEGAPNWPENDRFWRIIEEYAVTVFYTAPTAIRAFMKWGD
                     DWLKKHDLSSLRLLGTVGEPINPEAWMWYHKLVGGGRCPIVDTWWQTETGGHMITPIP
                     GATPTKPGTATLPFFGVDAAIVDDLGKEVGTNEQGKLVIRKPWPSMLRGIWGDKKRYQ
                     DTYWSEFKGWYFAGDGARRDKDGYIWIIGRIDDVLNVAGHRLGTAEVESALVSHPAVA
                     ESAVVGRPDELKGQGVVCFVTVKEGVKAGRELADALKSHVRKVIGPVATPDEIRFAAA
                     LPKTRSGKIMRRLLKQIAAGETLKGDTTTLEDINVIAALAAASGED"
     gene            2780..3223
                     /locus_tag="PLP58_11795"
     CDS             2780..3223
                     /locus_tag="PLP58_11795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ09247.1"
                     /translation="MDFADLFKRFGGKCFKTGQKLDIHARETWELDHILPSTWLYPMT
                     KANACLLSKEANQNKKAAWPSHFYTNNELIELARITGADLSLLASREPVVNPEIDVDA
                     CVTRYLKVREGGNLQKRVKELRDLLDSRGLSAGLSAANKRLLGHA"
     gene            complement(3231..5690)
                     /locus_tag="PLP58_11800"
     CDS             complement(3231..5690)
                     /locus_tag="PLP58_11800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ09248.1"
                     /translation="MNAYCLGRLGRHSGKAGLKGSIDMGVQATFRGGKDEPMHGWFPY
                     LEGYSPEFVRQMLSRYAPDARNVLDPFGGVGTTPLTAASLGLQAMYCEVNPLLQQLIR
                     AKADALGMTGEERRGHAAELEKLAARLEHDCHGFPTDAALRQTYTDTFVGSEFFDPPV
                     FETVLRLRSFLDHLQCGDQAHARFAEVAATACLLKASRLIRRGDVRFKTRAELVAGTE
                     DLLEAMIRHLLLMASDLRALPAPCDSPPALLCADARNLTLLPEQDIHAVLTSPPYING
                     TNYFRNTKVELWFMRCLRHGADLGAFRARAITGGINDVTKGKTWRETPAQAEKVVAEL
                     EKSAYDRRIPIMVAGYFADMAEVMDGMDVHLVPGAPVMIDIGDSNYGGVHVPAEKVLK
                     EMFAERGYQLVEDLTLRRRASRGGRELRQAMLVFKKSTARNRALCLREETGSVPWWNT
                     RWQKFKKELPHQHGVFAKRNWGHGLHSLCSYQGKMKPSIAHFLTRIFVPESGSMLDPF
                     GGVGTLPLEAALQGMRTWTFDISPAALCIARAKLGRPSMRECDQVLGAIEAWLDEARP
                     APEDSSRAALIRFNGPLESYFEPKTFEEILKVRRWFLTRPPRTDAEALVLACLLHILH
                     GNRPYALSRRSHPITPFAPTGDFEYRALLPRLREKIARTLDAELPPEFVTGQVLRQDA
                     CGFWPQEVRELDAVITSPPFYDSTRFYLSNWMRLWFCGWEAEDFKEQPKSFVDERQKK
                     SLDIYEPVFRQARERLKPGGVFVLHLGESRKCDMAAHLRHIARRWFRVADLFSESVSH
                     CESHGIRDKGTVTAHQFLVLE"
     gene            complement(6008..6421)
                     /locus_tag="PLP58_11805"
     CDS             complement(6008..6421)
                     /locus_tag="PLP58_11805"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009964159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HRJ09249.1"
                     /translation="MAITIRIDYQGELRCEATHGPSGNHFHTDAPVDNNGKGQSFSPT
                     DLVATALATCMATVIGIKARQKGYDLAGMKVSVEKHMSADSPRRITRLPVTIEIPLPP
                     DHPDRRLLEATALGCPVHHSVHPEIDKPVTFVWNG"
     gene            6604..7770
                     /locus_tag="PLP58_11810"
     CDS             6604..7770
                     /locus_tag="PLP58_11810"
                     /inference="COORDINATES: protein motif:HMM:NF012321.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HRJ09250.1"
                     /translation="MKTHTHPILQLTLKSILALAILPLAALAKPDKDKPGKPVNDDEA
                     LVQIALLLDTSNSMDGLIEQAKTQLWKIVNEFNDAKQDGKAAVVQVALYEYGNNNLSA
                     GTNYIRLVQPFTRDLDKVSEHLFKLTTNGGQEYCGAVVREAVDKLAWDAGAGVYKAVF
                     IAGNEPFTQGPVNPNDACRAAIQRGIVVNTIHCGSQAEGENGGWRTGAALAEGKFMTI
                     DQDKAIVRVQAPQDKEIARLSIELNKTYISYGKEGPAASANQMAQDQNAAANAASGAE
                     VQRAVTKISRNYDNSGWDLVDAVKRKRLAPGALKPEELPEEFKGLSEAEIKARIEAKA
                     AERERIQAEIKKLNDEREKFIAEKAREKGESDTLDVVIGKAVREQAAKVRIEFK"
     gene            7952..8638
                     /locus_tag="PLP58_11815"
     CDS             7952..8638
                     /locus_tag="PLP58_11815"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HRJ09251.1"
                     /translation="MPTLLVIEDDSAIRRGVCDALRFSGYEVLEAAEGISGMEQAAKA
                     SFDLMLLDLVLPNHNGFEILRALREHRPGTPVIILSARGEEADRVKGLKLGADDYVVK
                     PFSVRELLARVEAVLRRSPERPKPVHCVAFPCGEADVERRELRFADGSRAELSERECD
                     LIQYLASHRGRAISREELLRRVWRVEPRHTETRTIDMHIANLRAKLRDDGSNPKFLLT
                     VRGKGYMLAV"
     gene            8660..10636
                     /locus_tag="PLP58_11820"
     CDS             8660..10636
                     /locus_tag="PLP58_11820"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HRJ09252.1"
                     /translation="MLRWLAFLLCLSVFVAAMGWISLRALGLEEERRSDAIEAQIQER
                     MRLALWRMDALTSALLIRENARPPWHYQPFYQPDDAFTADNRALGKGEVVLPSPLLAG
                     LPDLVKLHFEKTHSGSLNSPQAPEGEERERALGWYAVSPQFQQGMDSLRALSGLLARH
                     PEFAVPTPAPQPQQQGKTASATPPAKQEVSKVDQLQADVNLREQMTRNTLITQNVGAE
                     KTEQFKLPLLKRAPEPKPGAQRASPAAAAAPSSRAPGSIAGGSSAQDAASSGEGAAAL
                     SSVASRYRANADAAAASPGAVLLQEKPALAGDMRPLWLGGELLLVRTAVVGGGKRLQG
                     VWLDWPGLRDRLYDTISDLLPDASLLPVPPESASTDPAALVTLPVRLVPGVVPVEFNA
                     PGSFLKPALALAWACLVAAAAAIAFVLHRAVTLSERRAAFVSAVTHELRTPLTTFRLY
                     SEMLADDMVPDAAQRRGYLQTLCDESSRLMHLVENVLSFSRIERGRTAARLEKVPAGG
                     LLDRILPRLCQRAAQADMRLELRVEENLRACEVRVDALAVEQILFNLTDNACKYAAPD
                     CDPRLLELSAVREAGTISLTLRDYGPGLPAGQMKKLFRPFSKSATEAAHSAPGVGLGL
                     ALSRRLARELGGDLRHLPVTGRGTAFCLTLPVAG"
     gene            complement(10643..12709)
                     /gene="ftsH"
                     /locus_tag="PLP58_11825"
     CDS             complement(10643..12709)
                     /gene="ftsH"
                     /locus_tag="PLP58_11825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009963595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HRJ09253.1"
                     /translation="MSDDTPPPNDPPNRRNQEPPFNWRGFLLFAMVGLLIVSALILNP
                     NSARSLEISYRDFIDIVTEDRIDKGKDLELVQQDTTTAEHIEGFYFKGTAASSESPGA
                     GTPAQGGGSIRFKVPVSIQFQKEELKALMDEHGLVLIPRYRTNHWGAMLFSMLPIFFL
                     LLLIYFLVRQQIKTAGRGALSFGKSRAKMLSQDRNKVTFKDVAGCDEAKEEVQELVEF
                     LKDPKRFQKLGGKIPKGVLMVGPPGTGKTLLAKAIAGEADVPFFTISGSDFVEMFVGV
                     GASRVRDMFEQGKKNAPCLIFIDEIDAVGRHRGHGLGGGHDEREQTLNALLVEMDGFD
                     SQEGIIIIAATNRPDVLDPALLRPGRFDRQVTVGLPDVRGREEILTVHAKKVKLSEHA
                     DLSKVARGTPGFSGAELANVINEAALLAARKNLKAITTAELEEARDKVRWGRERRSLA
                     LSDKEKENTAYHEAGHAILIELLEHTDPLHKVTIIPRGPSLGSTMWLPEEDKHNNRKS
                     ELLDELVVAMGGRVAEEIQFGDVTNGASGDIRMASRIARSMVCAWGMSDSLGMVEYGE
                     SDSAVFLARDLGRTRNYSDAMAQKIDEEVKRLIDEAYAKARAILLQHKDKLDAIAAAL
                     LEYETLDGEHIKEIMQHGRMLNPPPPPTKAPTPPPLPKNTDSRPVSNDDAEEDGLPGG
                     LAGVPA"
     gene            complement(12796..13365)
                     /gene="rlmB"
                     /locus_tag="PLP58_11830"
     CDS             complement(12796..13365)
                     /gene="rlmB"
                     /locus_tag="PLP58_11830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009959154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HRJ09254.1"
                     /translation="MPKPVHPARQNRHTHSTASAVRMHEEDDLPALLEGKTDPLVLVL
                     DCVQDPHNLGACLRTADAAGVACVVMPKDKSAPVSETVLRVSCGGAENVPLVRVTNLA
                     RAMDRLKKLGVWLVGTADEAAQSLYELDLKGPIGIVMGAEGEGMRRLTTEHCDFLAKI
                     PMRGSVPCLNVSVAAGVCLFEAVRQRIRA"
     gene            complement(13471..14991)
                     /gene="menD"
                     /locus_tag="PLP58_11835"
     CDS             complement(13471..14991)
                     /gene="menD"
                     /locus_tag="PLP58_11835"
                     /EC_number="2.2.1.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR00173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070204 -
                     2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-
                     carboxylic-acid synthase activity [Evidence IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-succinyl-5-enolpyruvyl-6-hydroxy-3-
                     cyclohexene-1-carboxylic-acid synthase"
                     /protein_id="HRJ09255.1"
                     /translation="MNTHIIRSLLTALARMGVSEICVAAGARNAPVIAALMESEGVKL
                     WNFFEERSAAFFALGRILADRAPVAVLTTSGTAAAELLPAVIEAHYQGLPLVLVTADR
                     PRRFRGSGAPQAIEQKDLFGPYAGPCLDVETGASLSWPTRTSGRPLHINLCLEEPLDT
                     NQPGIDFLAHHGPPPPKPPKPPVFQLPALPSAVLASGLSPLEADAAAPVLARMGLPVF
                     AEATANLWGAWPDRPDLAALLHPADDAALSGLDAAHILRLGGVPASRWWRDLENRPDI
                     HVTNVSRLPFPGLARREHVQTLPWTALNALGKYRLPAATVSLPPADALPPPLGETLGE
                     MAWMRALAASIPAGAQVFLGNSLPVREFNSVVPGIAPGVSFLANRGANGIDGVVSTWL
                     GASAIVRESWLVIGDLSALYDLAAPWVLPQMPAGNRRLVVINNGGGKIFSQVASLRSL
                     SKEARHIIENRHRVTFAPWAQMWGMSCMHAQAPEQLELLPNGPVLIEITPEDSVRN"
     gene            15199..15699
                     /locus_tag="PLP58_11840"
     CDS             15199..15699
                     /locus_tag="PLP58_11840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009962941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3D domain-containing protein"
                     /protein_id="HRJ09256.1"
                     /translation="MIRHSLLCIAALTALAKADKVQIKPSSTPVPPSQVIQGVRTTAY
                     THTESDHLKYGARTAAGTALKFGQVRSAAADWSVYPVGTVFQIVGNPALYIVEDYGSA
                     LVGTGTIDLYKPDQGRVNTWGVRRVNIQIVKWGSFSKSLAILKPRESKAPHVRQMVAR
                     IEARSV"
     gene            15796..16413
                     /locus_tag="PLP58_11845"
     CDS             15796..16413
                     /locus_tag="PLP58_11845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009958784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HRJ09257.1"
                     /translation="MQQKIDIPRKSIYRLSIYQRCLSRLKENGVDTVSSEALAKAAGV
                     KPTQLRKDLTYFGQFGTRGLGYNVDMLSGTISEVLGHNRLQPVILVGVGNLGSALLRY
                     GGFKKEGFEVVAAFDLEARRIPGISIPILGMEQMQEFIRDNQVKMAILTVPASSAQSV
                     TNKMVAAGMQAILNFSPTVLDVPENVVVNSVDLAVELENLSYFIR"
     gene            16465..>17046
                     /locus_tag="PLP58_11850"
     CDS             16465..>17046
                     /locus_tag="PLP58_11850"
                     /inference="COORDINATES: protein motif:HMM:NF015084.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ09258.1"
                     /translation="MESGEPFFNKLILHLDTAHPGAWNMAADQAWLEQSSAPVLRVYG
                     WDRATVTLGYAQSLAKLAGVLPDWPVVRRWTGGGVVLHEGDFTYSVIVPASDPWSCTQ
                     TLESYRRIHGSLAAALHEHGHTGCRLARKEDVVDAPFCFVAPAVHDVIRGPLKVAGAG
                     QRRGRLGLLHQGSVQQVRLKPEFWPFWAARLARS"
BASE COUNT         3067 a         5340 c         5607 g         3032 t
ORIGIN      
        1 tgtggtacca cgagcggctc tatcccgtgg tctggacgct ccaggctctg cgcgccgccg
       61 gcctgtccgt tcgcgtgtgt gagtgtgctc ctgctgcgtc tcagggattg actccaagca
      121 gccgtgcggc acaaaaacac ttcattccgt gattgcccat ccagcatctc tgaatgtagc
      181 ctcgcccttc catcgttaat ttccgccttc tcccaaccgt catgagccaa aaagccgtct
      241 cccaaaaaac cgcgaaagcc gccaaaaacg tcaagtccgc cacgacctcc accaaaagcc
      301 tgctcgttga aaaccgcgtg ttcaaacccg ctgccgagtt ttccagaaag gcccgcatca
      361 gctcgatggc ccactacagg aagatgcatg aggagtcgct gaggcatccg gagaagttct
      421 tcgcgcgcga ggcaaaggag ctggcctggc gcaagccctt caagaaggtg ctcgactgga
      481 agtgcccgaa cgcgaagtgg ttcaccgggg gccagctcaa tgtcagtgaa aactgcctcg
      541 accgccatct cgacggcccg cgcaagacaa aggccgccat catctgggaa ggcgagcccg
      601 gcgagaagcg cgtcatcacc taccagcagc tccaccggga cgtgtgccgt ttttccaacg
      661 tgctcaagcg ccacaaggtg aggaaaggcg accgcgtggt gatctacatg ccgatggtgc
      721 ccgaggccgc cgtggccatg ctggcctgca cgcgcatcgg cgcggttcac agcgtcgtgt
      781 ttggcggatt cagcgcggaa tccatcaagg accgcgtgct cgacagcggc gccacggtgg
      841 tcatcacctc cgacggaggc taccggcgcg gcgcggtggt tccgctcaag aagaatgtgg
      901 acgacgcttt gaagggcggg gacacgcccg tgaagacggt catcgtgctg cgccgcaccg
      961 gccaggatgt gcatatcgag gagggccgtg actactggtg gcaccgcgaa ctcgaatatg
     1021 tggatgcgaa ttgtcccgca gtggcgctcg attccgaggc tccgcttttc attctctaca
     1081 ccagcggcag cacgggcaag cccaagggca ttctgcacag cacggcgggc tatctgctgc
     1141 acgcgcagat gacctgcaaa tacgtcttcg acctgcgcga cgacgatgtg tactggtgca
     1201 ccgccgacat cggctgggtc acgggccaca gctacatggt ttacggccct ttggcgatgg
     1261 gagcgacttc attgatgtat gaaggcgcgc caaactggcc ggagaacgac cgcttctggc
     1321 gcatcatcga ggaatacgcg gtcaccgttt tctacaccgc gcccaccgcc atccgcgcct
     1381 tcatgaaatg gggcgatgac tggctcaaga agcacgacct ttccagcctg cgcctgctgg
     1441 gcactgtcgg cgagccgatc aaccccgagg cctggatgtg gtatcacaag ctcgtcggcg
     1501 gcgggaggtg ccccattgtg gacacctggt ggcagacgga gaccggtggc cacatgatca
     1561 caccgatccc cggcgccact ccgaccaagc cgggaactgc cacgctgccc ttcttcggcg
     1621 tggatgccgc catcgtggac gacctcggca aggaggtcgg caccaatgag caaggcaagc
     1681 tcgtcatccg caaaccgtgg ccatccatgc tgcgcggcat ctggggtgac aagaaacgct
     1741 accaggacac ttattggagc gagttcaaag gctggtactt cgccggcgac ggagcccggc
     1801 gcgacaaaga cggctacatc tggatcatcg gccgcattga tgacgtgctt aacgtcgccg
     1861 gtcaccgtct cggcacggcc gaggtggaga gcgcgctcgt ctcgcatccc gcagtcgccg
     1921 agtccgccgt cgtgggccgt ccggacgagt tgaaaggcca gggcgtcgtt tgctttgtca
     1981 ccgtgaagga aggtgtcaaa gccgggcgcg agctggccga tgcgctgaaa tcgcatgtgc
     2041 gcaaagttat cggccccgtc gccacgccgg acgagattcg ctttgctgcc gccttgccga
     2101 agacccgctc cggcaagatc atgcgccgcc tgctcaaaca aatcgccgcc ggcgagacgc
     2161 tcaagggcga caccaccacc cttgaggaca tcaacgtcat cgccgccctg gccgccgcga
     2221 gcggggagga ttgatctgtt ttgtcatggc gagacgcggc gcaaaaaaca gcactggcat
     2281 cgcgcgcctg gaaaacctcc aagcgcacga cgcctgggcc gggttcgttt gtgttcgctg
     2341 cggattgctt aacaccgtgc gcatcggtca aaagctgctc gcaccggatg atgccttgga
     2401 aagttgcagg tggtcatgcg ggaaatgcgg cttcgagcat ggcaaagatt cggctttgcc
     2461 ggactgggac aactggcccg gggaggccac tgcggcaggt tcggctcccg cgatgcggtt
     2521 ttggcagggt ttctttcgca tccacacgga aaatccgtca tcctattgga agcaatgcaa
     2581 cacctgcgga cgagtgctgc ctttccaagc gttcagcagg cacgccggat ggggaccgct
     2641 ggagcgccaa atggagtgcc gaagctgcaa gggggcgatc aacgcggtgc tcaatccgct
     2701 gcgcacgaaa gagcagatgc aggagtcagc gtcgcgccgc agggtctccg aactgctact
     2761 caaaggcgaa aatgagagaa tggatttcgc ggacctgttc aagcgttttg gcggcaaatg
     2821 tttcaagacc ggccaaaaac tcgacattca tgcgcgcgag acctgggagt tggaccacat
     2881 tcttccctca acatggctat accccatgac aaaggccaat gcctgcttgc tcagcaagga
     2941 ggccaatcaa aacaaaaaag ccgcgtggcc gagccatttc tacaccaaca acgagttgat
     3001 cgaactggcc cgcatcacag gcgcggattt gagcttgctg gcaagcaggg agcccgtggt
     3061 gaatccggag attgatgtgg acgcttgcgt cacgagatat ttaaaggtca gggagggcgg
     3121 caatcttcaa aagcgcgtga aagaattgag ggaccttctc gattcacgcg gactttcggc
     3181 aggactctcg gcggccaaca aacgcctgct gggccatgca tgaaccaagc tcactccagc
     3241 accaggaact gatgcgcggt cacggtgccc ttgtccctga tgccgtggga ttcgcagtgg
     3301 gagacacttt ctgaaaagag gtccgccacc cggaaccagc gccgcgcgat atgacgcaga
     3361 tgagccgcca tgtcgcactt tctgctttcc ccaagatgca gcacgaaaac gccgcccggt
     3421 tttaagcgct cgcgcgcctg cctgaagacc ggctcgtaaa tgtcgaggga ctttttctgg
     3481 cgctcgtcca cgaaagactt cggttgctcc ttgaaatcct ccgcctccca gccgcagaac
     3541 cagaggcgca tccagttgga gaggtaaaac cgagtgctgt cataaaatgg cggcgaggta
     3601 atgacagcgt ccaactcgcg gacttcctgc ggccagaatc cgcaggcatc ctggcgcagg
     3661 acctgcccgg tcacgaattc cgggggcagt tccgcatcca aagtgcgggc aatcttttcc
     3721 cgcaggcggg gcagcagcgc ccggtattca aagtcacccg tcggcgcgaa gggggtgatg
     3781 gggtgggagc ggcggctcag cgcatagggc ctgttgccgt gcaggatgtg cagcaggcat
     3841 gcaagcacca gcgcctccgc gtccgtgcgc ggaggccggg tcaaaaacca gcgcctgact
     3901 ttcaagattt cctcaaaagt cttcggctca aaataggact ccagaggacc gttgaaacgg
     3961 atcaatgctg cccggctcga gtcctccggt gcaggccgcg cctcatccag ccaggcttca
     4021 atagcgccca gcacctggtc gcattcccgc atggagggcc ggccaagttt ggcgcgggcg
     4081 atgcacagcg cagcggggct gatgtcaaat gtccacgtcc tcatcccctg cagggccgcc
     4141 tccagcggga gggtgcccac tccgccgaaa ggatccagca tggagcctga ctcgggcacg
     4201 aaaatgcgcg tcagaaagtg ggcgatggag ggtttcatct ttccctggta agagcacaac
     4261 gagtgcaggc cgtgccccca atttcttttg gcgaagacgc catgctggtg gggcagctct
     4321 tttttgaatt tttgccagcg tgtgttccac cagggcacgg agccggtttc ctccctcagg
     4381 caaagcgctc tgttgcgcgc ggttgacttt ttaaacacca gcatggcctg gcgtagttcc
     4441 cggccgccgc gcgaggcgcg ccggcgcaaa gtcaggtcct ccaccaactg gtagccgcgc
     4501 tcggcaaaca tttctttgag taccttctcg gctggaacat gcacgccgcc atagttcgag
     4561 tcgccgatgt caatcatgac cggcgcaccc gggaccagat gcacgtccat gccatccatc
     4621 acctcggcca tgtcggcgaa gtagccagcc accatgatgg ggatgcgcct gtcataagca
     4681 ctcttctcca actcggccac caccttctcc gcctgcgcgg gcgtctcgcg ccaggtcttg
     4741 ccctttgtga catcgttgat gccgcctgtg atcgccctgg cgcggaaggc gcccaagtca
     4801 gcgccatgcc tgaggcagcg catgaaccag agttccacct tcgtgttgcg gaagtagttg
     4861 gtgccgttga tgtaaggcgg gctggtcagc acggcatgaa tgtcctgttc cggcagcaag
     4921 gtcagatttc gcgcgtcagc gcagagcagc gcgggtggtg aatcacacgg cgccggcagc
     4981 gccctcaagt cggatgccat caggagaaga tgcctgatca tcgcctccaa taaatcctcg
     5041 gtccccgcca ccagttcagc gcgggttttg aagcgcacat ccccgcgccg gatgagccgt
     5101 gaggctttca gcaggcacgc ggtggcagcc acttcggcaa agcgcgcgtg tgcctggtcc
     5161 ccacattgca ggtggtccag aaacgagcgc agccgcagca cggtttcaaa caccggcggg
     5221 tcgaaaaatt cgctgccgac aaaagtgtcc gtgtaagttt ggcggagcgc cgcgtcagtc
     5281 ggaaagccgt ggcagtcatg ctcaagcctg gccgccagct tttccagctc cgccgcatga
     5341 cccctgcgtt cctccccggt catgcccaga gcatcggctt tggcccggat cagttgctga
     5401 agcagcgggt tgacctcgca atacatggcc tgcaatccaa ggctggcggc ggtcagcggg
     5461 gtggtgccga caccgccgaa tggatcaagg acattcctgg catctggggc gtaacgtgac
     5521 agcatttggc gcacgaattc cggcgagtag ccctccagat atgggaacca gccgtgcatc
     5581 ggctcgtcct tcccgccccg aaaagtggct tgcacaccca tgtcaatgct gcccttgagg
     5641 ccggctttgc cggaatgccg ccctagtctg cccagacagt aggcgttcac gcttctctgc
     5701 tcgcgcgcgg ccgccttttt tacggtcgcg atggtgcgat gcccgatcat gctccggacc
     5761 tcgacgctgg acaggtgtga aactcttttc cacaactccg tatcatcggc tgggaatgcc
     5821 agagtcaggc attgacggcg gccctccccg tccactagat catggaaata aatgctcttg
     5881 aagaactggc ctgccttttc cgaagtagat accataataa gaccgcattt cgggctgttt
     5941 cacaggaggg tcaaacgaca ttttgccttc ctgcaaattg tcgccgcggc ctcgttggtc
     6001 cgcgaactca cccgttccag acaaaggtga cgggtttgtc tatctccggg tggacgctgt
     6061 gatgcaccgg acagccgagg gcggtggcct ccagaaggcg gcggtcggga tggtcggggg
     6121 gcaggggaat ctcgatggtc acgggcaggc gcgtgatgcg ccgtggcgag tcggcgctca
     6181 tgtgcttctc cacactgacc ttcatgccgg cgagatcgta gcctttctgc cgggccttga
     6241 tgccgatgac ggtggccatg caggtcgcga gcgcggtcgc caccagatcg gtgggggaaa
     6301 aggactggcc tttgccgttg ttgtccacgg gggcgtccgt gtggaagtgg ttgcccgaag
     6361 ggccgtgcgt ggcttcgcag cgcagctcgc cctggtagtc tatgcggatg gtgattgcca
     6421 tggggatgag agtgaggagt ttgtgcgggc tgcggcaggg tgcattcctg gcgcgcgcac
     6481 tttttatcgc cagcggcggc actgctcaag cctggagtga aaaaggcggc gattcaggaa
     6541 gccccgcttc tttacagccg ctttacagac gcggggccgg atgggcggga ggctggggcc
     6601 gccatgaaaa cgcacaccca tcccatcctt caactcaccc tgaaatccat cctggctctg
     6661 gccattctgc ccctggcggc tctcgccaag ccggacaaag acaagcctgg caaacccgtc
     6721 aatgatgacg aggcgctggt gcagatcgcc ctgttgctgg acaccagcaa cagcatggac
     6781 ggtctcatcg agcaggcaaa gacccagctc tggaagatcg tcaacgaatt caacgacgcc
     6841 aaacaggacg gcaaagccgc cgtcgtgcag gtggcgctct atgagtacgg aaataacaac
     6901 ctcagtgccg gcacgaacta catccgcctc gtgcagccct tcacacggga tctcgacaag
     6961 gtctcggagc atctattcaa actcaccacc aacggcggcc aggaatactg cggcgcggtc
     7021 gtgcgcgagg cggtggacaa gctggcctgg gatgctggcg cgggggttta caaagccgtc
     7081 ttcatcgcgg gcaacgagcc tttcacccag gggccggtga atccgaacga cgcctgccgc
     7141 gccgccatcc agcgcggcat cgtggtgaac accatccact gcggcagcca ggccgagggg
     7201 gagaacggcg gctggcgcac cggcgcggcc ttggccgagg gcaagttcat gaccattgac
     7261 caggacaagg ccatcgtgcg cgtgcaggcg ccgcaggaca aggagatcgc ccgcttgagc
     7321 atcgaactca acaagaccta catcagctac ggcaaggaag gtccggcggc gagcgcgaac
     7381 cagatggctc aggatcaaaa cgccgcagcc aatgccgcat ccggggccga agtgcagcgc
     7441 gcggtcacaa aaatctcccg caactatgac aacagtggct gggacctcgt ggacgcggtg
     7501 aagcgcaagc gcctcgcccc cggcgcgctg aagcccgagg aattgccgga ggagttcaaa
     7561 ggattgagtg aggccgagat caaggcgcga atcgaagcca aggccgccga gcgcgagcgc
     7621 atccaggctg agatcaagaa gctgaatgat gagcgtgaaa agttcatcgc ggagaaggcc
     7681 agggagaaag gcgagtccga cacgcttgat gtcgtcatcg gcaaggccgt gcgcgagcag
     7741 gcggcgaagg tgaggatcga gttcaagtaa aggggtgcca ccgttggagt tcatgcttca
     7801 gcatgtccgg gagcgggtgc gcggcggcgc gcgctcccgt gtcgcctgaa ggcttcactc
     7861 cagcacgggc aatcaagaga cgactgcccc gccttggagc cttgcccagg ctcgcgtttt
     7921 gaaggacact tccacgaaaa ttcaaaccat catgcccact cttcttgtga tcgaggacga
     7981 cagcgccata cggcgcggtg tctgtgacgc gctgcgcttc tcgggctacg aggtgctgga
     8041 ggccgcggag ggcatcagcg gcatggagca ggcggcgaaa gccagttttg acctcatgct
     8101 gctggatctc gtgctgccca accacaacgg cttcgagatt ctccgcgctc tgcgcgagca
     8161 ccggccgggc acacccgtca tcattctctc cgcgcgcggg gaggaggctg accgcgtcaa
     8221 agggctcaaa ctcggcgcgg atgactatgt ggtgaagccc ttcagcgtgc gcgagctgct
     8281 ggcgcgcgtg gaggccgtgc tgcgccgctc gccggagcgg cccaaacctg tgcattgcgt
     8341 ggcatttcca tgcggcgagg cggacgtgga gagacgcgag cttcgcttcg ccgacggatc
     8401 acgcgcggag ctctccgaac gtgagtgtga tctcatccag tatctggcct cgcatcgggg
     8461 gcgcgccatc tcacgggagg agctgctgcg gcgcgtctgg cgcgtcgagc cgcgccacac
     8521 cgagacgcgc accattgaca tgcacatcgc caacctgcgc gcgaagctgc gggatgacgg
     8581 ctcaaatccg aagtttctcc tgaccgttcg tggtaaagga tacatgctgg ccgtttgaaa
     8641 ctcgaagtcc ctgatcccca tgctccgctg gctcgctttc ctcctttgcc tttccgtctt
     8701 cgtggcggcc atgggctgga tcagtctgcg cgctttggga ctcgaggagg agcgccggtc
     8761 ggatgccatc gaggcgcaaa tccaggagcg catgcggctg gctctctggc gcatggacgc
     8821 gcttaccagc gcgctgctga ttcgggaaaa cgcgcgccca ccctggcatt accagccctt
     8881 ctaccagccg gatgatgctt tcacagcgga caaccgcgct ctgggcaaag gcgaggtcgt
     8941 tctgccttcg ccgctgctgg ccgggctgcc cgacctggtg aaactgcatt ttgaaaaaac
     9001 acacagcggc tccctcaaca gcccccaggc tccggagggt gaggaacgcg agcgcgcgct
     9061 gggctggtat gcggtgagtc cgcagttcca gcagggcatg gacagtctgc gcgctctttc
     9121 cggcctgctc gccaggcacc cggagtttgc cgtgcccaca ccggctccgc agccgcagca
     9181 gcaaggcaag acggcatccg ccactccacc cgccaaacag gaagtgtcga aagtggacca
     9241 actccaggcc gatgtgaacc tgcgcgagca gatgacacgc aacacgctga tcacccaaaa
     9301 cgtgggcgcg gagaaaacgg agcagttcaa gctgccgctg ctcaagcgcg cgcccgagcc
     9361 gaagccgggc gcgcagcgcg cttcacctgc cgccgccgca gcgccctcca gccgcgctcc
     9421 cggcagcatt gcaggtggca gttctgccca ggatgcagcc agctcaggcg agggcgcggc
     9481 ggcattgtcc tcggtggcgt ccaggtatcg cgcgaacgct gatgccgccg ccgcatcgcc
     9541 gggcgcggtg ctgcttcagg agaaacccgc gctggcgggt gacatgcgcc cgttgtggct
     9601 tggcggggag ttgcttctgg tgcgcaccgc tgttgtcggg ggcgggaaaa ggctgcaggg
     9661 ggtttggctt gactggccgg ggctgcggga ccgcctttat gacaccatca gtgacctgct
     9721 gccggatgcc agcctgctgc cggtgccgcc tgagtccgcc agcaccgatc ctgccgcgct
     9781 tgtcacactt cctgtcaggc tcgtccctgg ggtggtgccg gtggagttca atgcccctgg
     9841 ctcgtttttg aaacccgcgc tggcactggc atgggcctgc cttgtcgcgg cggcggcggc
     9901 catcgccttc gtgctgcaca gggcggtgac gttgagcgag cggcgcgcag cctttgtctc
     9961 ggcggtgacg cacgagctgc gcaccccttt gacgactttc cgtctttact cggagatgct
    10021 ggctgatgac atggtgccgg atgccgctca gcgcaggggt tatttgcaga cgctctgtga
    10081 cgagtcgagc aggctcatgc atttggtgga aaacgttctg tccttttcac gaatcgagcg
    10141 cggacgcaca gccgcacgtc tggagaaggt gccggccggc ggtttgctgg accgcattct
    10201 gccgcgcttg tgccagcgcg cggcgcaggc ggacatgcgg ctggagttgc gtgtggagga
    10261 aaacctgcgc gcttgcgagg tgcgggtgga tgccctggcc gtggagcaga ttcttttcaa
    10321 tctcaccgac aacgcctgca aatacgccgc gccagactgt gatccgcgtc tgttggagtt
    10381 gagcgccgtc agggaggctg ggacgatctc gctgactctg cgggattacg gccccggact
    10441 gccggcgggt cagatgaaaa agctgttccg ccccttcagt aaatcggcca ccgaggcggc
    10501 gcacagcgcg ccgggagtgg ggctcggact tgcgttgagc cggcggctag cgcgcgagct
    10561 tggcggggat ttgaggcacc tgccagtgac ggggcgcggc acggcttttt gcctgacgct
    10621 gccggtggcg gggtgagatg gctcacgccg gcacgcctgc gaggccgcca ggcaggccgt
    10681 cctcctccgc gtcgtcattg ctgaccgggc gggagtcggt gttcttgggc agcggcggcg
    10741 gtgtcggggc cttggtgggc ggcggcggcg gattgagcat gcggccatgc tgcatgatct
    10801 ccttgatgtg ctcgccgtcg agcgtctcgt attcgagcag ggctgcggcg atggcatcca
    10861 gcttgtcctt gtgctggaga aggatcgccc tggccttggc ataggcctcg tcaatgaggc
    10921 gcttgacctc ctcgtcaatt ttctgcgcca tggcgtcact gtagttgcgg gtgcggccca
    10981 ggtcgcgggc caggaagacg gcgctgtcgc tctccccgta ttccaccatg ccgaggctgt
    11041 cgctcatgcc ccaggcgcag accatgctgc gggcgatgcg cgaggccatg cggatgtctc
    11101 cgctggcgcc gttggtcaca tcgccgaact ggatttcctc cgccacgcgc ccgcccatgg
    11161 ccacgacgag ttcgtcgagc agctcgcttt tgcggttgtt gtgcttgtcc tcctcgggca
    11221 gccacatggt ggagccaagg gaggggccgc gcgggatgat ggtgactttg tgcagcgggt
    11281 cggtgtgctc cagcaactcg atgagaatgg cgtggccggc ctcgtggtag gcggtgtttt
    11341 ccttttcctt gtcgctcagc gccaggctgc ggcgctcgcg gccccagcgc accttgtcgc
    11401 gcgcctcctc gagctcggcg gtggtgatgg ccttgaggtt tttgcgggcg gcgagcaggg
    11461 cggcctcgtt gatgacgttg gcgagttccg cgccggagaa accgggggtg ccgcgcgcga
    11521 ccttgctgag gtcggcatgt tcggagagct tcactttttt ggcgtggacg gtgaggattt
    11581 cctcgcgccc gcgcacgtcc gggaggccga cggtgacctg gcggtcaaag cggccgggac
    11641 gcagcaaggc ggggtccagc acgtccggcc ggttggtggc ggcgatgatg atgatgcctt
    11701 cctgggagtc aaagccgtcc atctcgacga gcagggcgtt cagcgtctgc tcgcgctcgt
    11761 cgtggcctcc gcccaggccg tggccgcggt gccgtcccac ggcgtcaatc tcgtcaatga
    11821 agatgaggca gggcgcgttt tttttgccct gctcgaacat gtcgcgcacg cggctggcgc
    11881 cgacgccgac gaacatctcg acaaagtccg agccgctgat ggtgaagaag ggcacgtcgg
    11941 cctctccggc gatggccttg gccagcaggg tcttgccggt gcctggaggg ccgaccatga
    12001 gcacgccttt ggggatcttg ccgccgagct tctgaaagcg cttcgggtct ttgaggaatt
    12061 cgacgagctc ctgcacctct tccttggcct cgtcacagcc ggccacgtct ttgaaggtga
    12121 ccttgttgcg gtcctgcgag agcattttgg cgcggctttt gccgaagctc agcgcgccgc
    12181 gtccggcagt cttgatctgc tggcgcacca ggaagtagat gagcagcagg aggaaaaaga
    12241 tgggcagcat ggagaagagc atggcgcccc agtgattggt gcggtagcgc gggatgagca
    12301 ccaggccgtg ctcgtccatg agcgccttga gctcctcttt ctggaactgg atgctcaccg
    12361 gcaccttgaa gcggatggag ccgccgccct gggcgggagt gccggcgccc gggctttcag
    12421 acgaggcggc ggtgcctttg aaataaaaac cctcgatgtg ctccgccgtg gtcgtgtcct
    12481 gctgcacgag ttcgaggtcc ttgcctttgt ctatccggtc ctcggtgacg atgtcaatga
    12541 agtcgcggta ggagatttcg agggagcgcg ccgagttggg gttcaatatc agggcggaga
    12601 cgatgagcag cccgaccatg gcaaagagca ggaagccgcg ccagttgaag ggcggctcct
    12661 ggttgcggcg gttgggcgga tcgttcgggg gtggggtgtc gtcggacatg ggggcgttga
    12721 ttcaattacg cgcgccgggc gcttgcgccc cctgcggttg tgttttttgg actggtgcgc
    12781 gggatcgcgg tcatttcaag cgcgtatccg ctggcgcacg gcctcgaaca aacaaacgcc
    12841 cgcggccacg gagacattca ggcacggcac gctgccgcgc atggggatct tcgccaggaa
    12901 atcgcagtgc tccgtcgtca gccgcctcat gccctcgccc tcggcgccca tgacgatgcc
    12961 gatggggcct ttcagatcga gttcgtaaag cgactgcgcg gcttcatcgg cggtgcccac
    13021 gagccagacg cccagctttt tgagccggtc catggcgcgt gccagattgg tcacgcgcac
    13081 cagcggcaca ttctccgcgc cgccgcagga gacgcgcagc actgtttcgg agacgggggc
    13141 ggacttgtcc tttggcatga ccacgcaggc cacgcccgcc gcgtcggcgg tgcgcaggca
    13201 ggcgccgaga ttgtgcgggt cctgcacgca gtcgagcacg agcacgagcg ggtccgtctt
    13261 tccctcaagc agggctggca ggtcatcctc ctcatgcatg cgcaccgcgc tggcggtgga
    13321 gtgcgtgtgg cggttctggc gcgcggggtg gacgggcttg ggcatgcggg atggaagatc
    13381 ggaattgaaa aatcgaaaca cgaaaatcac aaagatggcc gcggcccgac gcgcgcgttc
    13441 ccgggcggag gtcggagcaa tgaccgcgag tcaattcctc acggagtcct cgggtgtgat
    13501 ttcgatgagc acggggccgt tcggcagaag ttcgagctgc tccggcgcct gcgcgtgcat
    13561 gcagctcatg ccccacattt gcgcccaggg ggcgaaggtg acacggtggc ggttttcgat
    13621 gatgtggcgg gcctctttgg acagggagcg gagcgaggcg acctgggaaa agattttgcc
    13681 gccgccgttg ttgatgacga ccaggcggcg gtttcccgcg ggcatctgcg gcagcaccca
    13741 gggcgcggcg agatcgtaga gggcggagag gtcgccgatg acgagccagg actcgcgcac
    13801 gatggcgctg gcgccgagcc aggtggagac gacgccgtca atgccgttgg cgccgcggtt
    13861 ggccaggaag ctcacgcccg gcgcgatgcc gggcacgacg ctgttgaact cacgcacggg
    13921 caggctgttg cccaggaaga cctgcgcgcc ggccgggatg ctggcggcca gggcgcgcat
    13981 ccaggccatc tctcccagtg tctctcccag tggcggcggc agggcatccg caggcggcag
    14041 tgacaccgtg gcggcgggca ggcggtattt gcccagggcg ttgagagccg tccacggcag
    14101 tgtctgcaca tgctcccggc gcgccagccc aggaaagggc agccgggaga cattggtgac
    14161 gtggatgtcc gggcggtttt ccaagtcccg ccaccagcgg ctggccggca cgccgccgag
    14221 gcgcaggatg tgcgcggcat caagtccgga gagcgccgcg tcatcggcgg gatgcagcaa
    14281 cgcggcgagg tccgggcggt ccggccacgc gccccagaga ttcgcggtgg cttcggcgaa
    14341 gacaggcagt cccatccggg ccagcacggg cgcggcggca tccgcctcca gggggctgag
    14401 gccggaggcg agcacggcgg agggcagcgc gggcagttgg aagacgggcg gctttggcgg
    14461 tttcggcggc ggcggtccgt ggtgcgccag gaaatcaatg ccgggctggt tggtgtcgag
    14521 cggctcctcg aggcagagat tgatgtgcag ggggcgtccg ctggtgcgtg tcggccagga
    14581 caggctggct ccggtctcga catccaggca ggggccggca tacgggccga agaggtcttt
    14641 ttgctcgatg gcctgcggcg cgccgctgcc gcggaagcgt ctcgggcggt cggcggtgac
    14701 gagcaccagc ggcaggccct ggtagtgcgc ctcaatgacg gcgggcagga gctccgccgc
    14761 cgccgtgccg gaggtggtga gcacggccac cggcgccctg tcggccagga tgcgccccag
    14821 ggcgaagaag gcggcgctgc gctcctcgaa gaagttccag agctttaccc cctcgctctc
    14881 catgagcgcg gcgatcaccg gcgcgttgcg cgcgcccgcc gccacgcaga tttccgagac
    14941 tcccatgcgc gccagcgcgg tgagcaggga gcggatgatg tgggtgttca tgcgggagtc
    15001 cggccttgtc tccaccatcc gaccccggcc ttcaaccgca aatgtcccgc gccggtctga
    15061 ctttcgggat ttccagacta agaacgccct ggagaatcgg agcttgacaa tcccggcctc
    15121 atcggcacca ttcgcgcccc tcggaccgcc agccccggct gcggtccgtg cgcgacctga
    15181 acaaccccga cccatccatt gatccgtcat tccctcctgt gcatcgccgc gctgaccgcc
    15241 ctcgccaagg cggataaagt ccaaatcaag ccgtcttcga ctccagtgcc gccgagccag
    15301 gtgatccagg gtgtgcgcac caccgcctac acccacaccg agtccgacca tctcaaatac
    15361 ggagcgcgca cggcggcggg caccgccttg aagttcggcc aggtgcgcag cgcggcggcg
    15421 gactggtcgg tgtatccggt cggcaccgtt ttccagatcg tcggcaatcc ggcgctctac
    15481 atcgtggagg actacggctc cgccctggtg ggcaccggca cgattgacct ctacaagccc
    15541 gaccagggcc gtgtgaacac ctggggagtg cggcgggtga acatccagat cgtcaaatgg
    15601 ggcagctttt ccaaaagcct cgccatcttg aagccgcgcg agagcaaggc cccgcatgtc
    15661 cgccagatgg tggcccggat cgaggcgcgc tcggtctgag cgcgcaccgc gccgcgcgtc
    15721 atggaatgga tgcgcggcgg gcctgagggc aggacgggtt attgacaatc gagccgccag
    15781 cgtgaaattt cacaagtgca gcagaaaata gacatccccc gcaagtccat ttaccgcctg
    15841 tccatctacc agcgctgcct gagccggttg aaggagaacg gcgtggacac cgtgtcttcc
    15901 gaggcgctcg ccaaagccgc cggggtcaag cccacccagt tgcgcaaaga cctcacctac
    15961 ttcggccagt tcggcacgcg cgggctgggc tataatgtgg acatgctcag cggcaccatc
    16021 tccgaggtgc tcggccataa ccgcctgcag cccgtcatcc tcgtcggcgt cggcaacctg
    16081 ggttccgcgc tcctgcgcta cggcggcttc aaaaaggagg gcttcgaggt ggtggccgcc
    16141 tttgacctgg aggcgcgccg catccccggc atcagcatcc ccatcctcgg catggagcag
    16201 atgcaggagt tcatccgcga caaccaggtc aagatggcca tcctcaccgt ccccgccagc
    16261 agcgcccaga gcgtgaccaa caaaatggtg gccgccggca tgcaggccat cctgaacttc
    16321 tcccccaccg tgctcgacgt gccggaaaac gtggtcgtca acagcgtgga cctcgccgtc
    16381 gagctggaga acctcagcta cttcatccgc tgagcgcgcg catccgcgca tgacagccgc
    16441 ccggcttgtg cttcatcttc ccgcgtggaa tccggcgaac cgttttttaa caaactcatc
    16501 ctgcacctcg acacggcgca ccccggcgcc tggaacatgg ccgcggacca ggcctggctc
    16561 gagcaaagct ccgcgcccgt gctgcgcgtc tatggctggg accgcgccac cgtcaccctc
    16621 ggctatgcgc aaagcctggc aaagctggcc ggcgtcctgc cggactggcc cgtcgtgcgc
    16681 cgctggaccg gcggcggcgt cgtgctgcat gagggcgact tcacctactc ggtcatcgtg
    16741 cccgccagcg acccgtggtc atgcacccag acgctggaga gctaccgccg cattcacggc
    16801 agcctggccg ccgccctgca tgagcacggc cacaccggct gccgtctggc ccgcaaggag
    16861 gacgtggtgg acgcgccttt ctgcttcgtg gccccggcgg tgcatgatgt catccgcgga
    16921 ccgttgaagg tggccggcgc gggccagcgg cgcggcaggc tcggcctgct ccaccagggc
    16981 agcgtgcagc aggtgcggct caagccggag ttctggccct tctgggccgc gcggctcgcg
    17041 cgctcc
//
LOCUS       DAOMYJ010000102        17043 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Prosthecobacter sp. isolate AMR_MDS_5629
            NODE_1160_length_17043_cov_20.860137, whole genome shotgun
            sequence.
ACCESSION   DAOMYJ010000102 DAOMYJ010000000
VERSION     DAOMYJ010000102.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867850
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prosthecobacter sp. (wastewater metagenome)
  ORGANISM  Prosthecobacter sp.
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Verrucomicrobiaceae; Prosthecobacter.
REFERENCE   1  (bases 1 to 17043)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17043)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.30
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:34:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,742
            CDSs (total)                      :: 4,684
            Genes (coding)                    :: 4,676
            CDSs (with protein)               :: 4,676
            Genes (RNA)                       :: 58
            tRNAs                             :: 55
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17043
                     /organism="Prosthecobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1160_length_17043_cov_20.860137"
                     /isolate="AMR_MDS_5629"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1965333"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..870
                     /locus_tag="PLP58_11855"
     CDS             <1..870
                     /locus_tag="PLP58_11855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ09259.1"
                     /translation="QAGRDHVVAEYNENSRGDRHPMRAIMTREHGYIFNPWSDGRRVM
                     TTATQGTVTYRRMKALAASDAGIAARLNMIDHRAPEELYHYAYDPDALTNLIGRADHA
                     ETQARLARMLEEWMVRTGDPMLEVFRQRGDPAVLDAYVTRQQAEAEARRAGKKGGARG
                     AKKAAAKGKSAMPPENAETKKGARGRGETGLIEIRLPESARRGGEMTAVIPHSLPAGL
                     GVRKLHVTLKDAAGKRLERKVIEITGKAEARVSFTLPPGYAGESVSVAAFVGEEYEKS
                     LQHLQSDAVPVVP"
     gene            complement(876..1862)
                     /locus_tag="PLP58_11860"
     CDS             complement(876..1862)
                     /locus_tag="PLP58_11860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ09260.1"
                     /translation="MKHLIIIHGRSTKPSGSEKERLVKKALLHGLKREHGDAAKKIET
                     GKVECSHAYYGDINNRLMIAAGQKKKSDLPGVNDPGYGSGPCELPGSYDDDLDKLLAR
                     KTTAFKKADYQKLLDEHKDRRWKDEAASVLSAVAGIFGMNDQLVAKATPDMGAYLLSR
                     KAGSEVRQRLAIHLKPALLAGDDICLVSHSMGCIVSYDTLWKYSQMSEYKDIQDTGNR
                     VSLWLTLGCPLGEPGVIKNLYDAHEPADGKYPCRIIRRWENVAAHDDFVSHDEGVADD
                     FKEMRRLGYLESIKDRRIYNFWTGSDGANPHKFYGYLDHPEVAALIAGWIAA"
     gene            2060..3913
                     /gene="glmS"
                     /locus_tag="PLP58_11865"
     CDS             2060..3913
                     /gene="glmS"
                     /locus_tag="PLP58_11865"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009962724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004360 - glutamine-fructose-6-phosphate
                     transaminase (isomerizing) activity [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HRJ09261.1"
                     /translation="MCGIVGYIGRRQASPILLDGLRRLEYRGYDSAGIALQNNGSGLN
                     FIKRAGRIDNLSKAVAEASPQGSTGISHTRWATHGAPTDENAHPHRDQSGRLALVHNG
                     VIENYQTLRDQLIAQGHTFESQTDTEVLAHLVGRYFDASDEKDGPTRLRKALQSALAK
                     VKGTYGIAAMHADVPGVLVGARLGSPLVVGLGDEEHFLASDVSAIVAHTRDVVYLNDH
                     DIVSLTEGRYDVTSMAGGEAQVKVSRVEFTADDAEKGDFPHFMLKEIYEQPKSLQNAM
                     RGRLSRDEATAILGGLNMTPQELRGVDRIILSGCGTAFHAAMVGEYLIETLARVPTEV
                     EIASEFRYRNVPADRNTLQFVLSQSGETIDTLAALREARRKGIRCLGIVNSVGSTIAR
                     ESDGGCYIHAGPEIGVAATKTFTSQVMIMNLLGLLFGRIHHLSSADGIEMIDELEAIP
                     DKITAILKQTDKIKAIAEKHANAEGMLFMGRLQNYPVAMEGALKMKEISYIYASGHPS
                     AELKHGIIALVKPDMPSIFIAPDDAVFDKNVSNIEEVLARKGPVIAVTTEGRTELERL
                     TEDVIYVPECPSYLSPLLTVIPMQLLAYHTAVARGCDVDKPRNLAKSVTVE"
     gene            3989..4825
                     /locus_tag="PLP58_11870"
     CDS             3989..4825
                     /locus_tag="PLP58_11870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ09262.1"
                     /translation="MTPLQTLPVLGAALLAALQGHAQAPPAMPAERSPDTIAAGAGEF
                     VARAVFWTEDQTPADFLDLAGQHTKARWRTLYRPPPPGPMADRVKAAFTLGSLVGETF
                     LIWQAGDAQQFRNNNQELATFCRILGLGEKMRPRLMAQAKMAEMDEWKDMRQEIVDGH
                     QEIMRTLREQMDDDLALLVDIGAWMRALEIVSKLVQETPEVDKRPLCIGSPALLADMR
                     QDFAKLTPENRRSPLLQPVIAVISELERVWGDPKIGAPTQNMVIRTHEQLRAVMEELT
                     LR"
     gene            4975..6552
                     /locus_tag="PLP58_11875"
     CDS             4975..6552
                     /locus_tag="PLP58_11875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ09263.1"
                     /translation="MKHTTTLLAAALLGLGLHHTAMAGSNNFASATDLTTHNWPPAVS
                     LAAFTSESGEPGHRPNGSNGAQKSAWWKWTAPHNGFCTVDTMGHSDDQFIYDTLIAVY
                     TGTDVNALTRVAGNDDHDSHLGYGSANSSSVTFYATEGATYHFAVDGRSAAAINASNH
                     KAQLRLRLLRARAEHRVGVISIPLEEFMHGMIQFSKTAGHTFSGKILINGKSHPFKGV
                     FGLDGYCSISIERIVPRGAAPQPPLTILLDGTQSIANGSPFTEFALISHLINGAWWGS
                     MATRQTFAKGTESGLKGRYSAAISAPDNVWKSGVMSFTATGAGVVKGASVLPDGTKTT
                     FGSFLCENDASSSWVPVYNGLYKNAGYFASRLKLTEAGATDQVTSVNVFGQHHRPEAP
                     GSLFMPDGRTFFTSVVVKGSTYTPPAAKARALGFLDGSMGAGKLSIPMSSGEINPAIM
                     ENLTLDTGNKIIFASKARKPALTLNKSTGLVTGYLLDDAGKKRSLTGVLFMDGMTPKL
                     EGHVSGATKNSFFEVIP"
     gene            6811..8085
                     /locus_tag="PLP58_11880"
     CDS             6811..8085
                     /locus_tag="PLP58_11880"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HRJ09264.1"
                     /translation="MKRTPLTHRLAMMILAASSSSASAQSTAGPERQNLLGWAEGAFT
                     IRTPSPPRTEAHVVALDGQPATTTIGVPKNAPLPHEFVIELPASTTFTSFAVPEIGEF
                     GPAKGRHVKTVEIAGSSESAESGFSPLAGFEIQIEKKAPQEFPVHSPRPVRWLKVRFL
                     DRFTPQTSPADPVLFSELMGYGAQEQKEPPEKGFNGVWTLRRGYEVSRNLIELRQDES
                     GQVTGCQVLGGQLSKISGSVEDGIVRWISTSVQGGRNVSVPSIARITSEGELHGVSSF
                     HGGLQTFSGIPAPQGATTPCSEAPESENAVSAALQAGLAAVLYGIHFDVDSDVLRPDA
                     APALEQILEALEMNPSIAVIIEGHTDSDGSDTHNLDLSKRRAAAVVRWLAGRKVDAAR
                     LEASGKGESTPIADNASAVGRAMNRRVEVRPR"
     gene            8557..8784
                     /locus_tag="PLP58_11885"
     CDS             8557..8784
                     /locus_tag="PLP58_11885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ09265.1"
                     /translation="MTSKQKHHRLKLVLEKTPPILEASKPASDGLAKLVWAIGLTTGF
                     MILAWRLPETALDAGIVTRAMKYALHKLTRE"
     gene            8893..9342
                     /locus_tag="PLP58_11890"
     CDS             8893..9342
                     /locus_tag="PLP58_11890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007416624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HRJ09266.1"
                     /translation="MTPECILLIGIQAAGKSTFAAERFAQTHVILNLDTLRTRKREER
                     LLLECLEQRRSFVVDNTNVSRAERARYLPLAGEAGYRRVGYAFEAAIGPALERNARRE
                     RVVPPYAVQATRNRLQWPALDEGFDELHFVRLAGNGFRIEPWEPHEI"
     gene            9332..10732
                     /locus_tag="PLP58_11895"
     CDS             9332..10732
                     /locus_tag="PLP58_11895"
                     /inference="COORDINATES: protein motif:HMM:NF016341.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008193 - tRNA guanylyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA(His) guanylyltransferase Thg1 family
                     protein"
                     /protein_id="HRJ09267.1"
                     /translation="MKFDDLDREMRRFETERDWQVPEGVFIIARMDGRGFTRLTKEVM
                     RFEAPYDERFRDAIAATARHLMNCGFRALHAFCQSDEISLLLHPRDGTFQRKERKLLS
                     VLAGEASAAITHALGRPACMDSRLCLLPDSARVADYFRWRMEDARRNCLNSHACWLLR
                     RLGRDATAAHNEIEGLGVEEKMALLAGHGIVFDELPAWQRRGFAVEWREEEKAGMDPR
                     TGTATVTTRRRLQTILDLPDGAEYGQLILTLLRSVPDDESRMHHLAGTLTHFQVPAPI
                     EDFYDDGMLRVHIERKPGTRLAHGFCFLQHPSGRRSWQGWFKEGVRSGQWVYWKDTDE
                     AHVGEVTEFGDAEYTPMHSRPLDGAALSPERVILISEEYGYRSWWWWPDRPMAEIICW
                     WKDLPTVEQWSLSGPVLPGSTIEVAEDESAAFHQKWMDHLHIRLHLHEDDDSVLHLPG
                     GETVLHQGHPEAALQL"
     gene            10815..12209
                     /locus_tag="PLP58_11900"
     CDS             10815..12209
                     /locus_tag="PLP58_11900"
                     /inference="COORDINATES: protein motif:HMM:TIGR00225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HRJ09268.1"
                     /translation="MMHFPAMRVSLAAALVFLPILCLRAQEPAPDAEPETAPALKVPP
                     KAAPPAATAKPPPEDAYRQMELLTRAMEMIRQNYVDEDKVTFAELVEGALEGMLRRLD
                     PHCEYMGRTLFEDMQREHSDTSEGIGITVALREGALTIITVREDGPAGRAGVLAGDQM
                     VRINDVLTDSVGVAEAIQLLQGKSGEALKLTIRRPGTRQFLEFSMEREVLAETSVHDA
                     MLLTPKLAGGAKVGYARISQFTQSTARDLSAALDELESADMQAFVLDLRNNPGGLLDS
                     AVAVCGEFLPAGTVVVTTEGRIASQNPPPYRTPARDGKKPRRYPVAVLVNHASASASE
                     IVAGALQDLKRAIVVGTTSFGKGSVQSILPMKNGAAMRLTTAKYYTPSHRTIHEHGVT
                     PDILSNLTPAEEQRVAKWRTQHGTGEAAAIEIANLGDKQLERAVDALKGVLVFDAFGS
                     GEAALAPRALPAGQ"
     gene            12225..13421
                     /locus_tag="PLP58_11905"
     CDS             12225..13421
                     /locus_tag="PLP58_11905"
                     /inference="COORDINATES: protein motif:HMM:NF024968.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HRJ09269.1"
                     /translation="MRPQRILILVENLPVPLDRRVWQEACALRDAGHEVTVICPRMRG
                     HTQPEEKLDGIQIYRHWITGEAGGITGFLGEYASALCGEFLCALKAWRRTGFDVIHLC
                     NPPDLIFLVALPFKLLAGVRVIYDVHDLWPEMFEAKFARRGLLYRAVRLAERCTLALA
                     DAVIATNESVRAVVKKRGRKSDDEVFTVRTAPDKMDTSAPEDPALKKGRPHLVGYIGV
                     MGSADGVDYLLRAAHHILHTRQRQDVQFLLMGGGPEHERLLALREELGLAAHVDMPGR
                     VSQAFLCTALRTMDLGAACDPINAYNDHCTMNKTLEYMAFGKAQVMFGIHEGRESAGE
                     AALYVMENSPEQLGDAILELLDDPPRRHRMGQLGHQRLHGPLSWPHSVRQLLRAYARA
                     LAGKAP"
     gene            complement(13530..13808)
                     /locus_tag="PLP58_11910"
     CDS             complement(13530..13808)
                     /locus_tag="PLP58_11910"
                     /inference="COORDINATES: protein motif:HMM:NF016876.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HRJ09270.1"
                     /translation="MPTHSKNDNAGAWYEERRQRLGVEFTEAVEAGVAAILKTPDRCQ
                     QVGGGVRIFRLKRFPYYIFYRYNERGQHVRIVAVMHHKRRPDYWRERL"
     gene            complement(14799..15149)
                     /locus_tag="PLP58_11915"
     CDS             complement(14799..15149)
                     /locus_tag="PLP58_11915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ09271.1"
                     /translation="MKTTLRFIPLVAVVVLAFSPFSKARDPEFVALAGWVNTPELNAK
                     EGRRVHTILTEHKIENVAVGSAGMTLNVLKPRAAEGRKLLAKAILNEGLRIVLYDEHG
                     KMVSPETVLGQKKQ"
     gene            complement(15292..15663)
                     /locus_tag="PLP58_11920"
     CDS             complement(15292..15663)
                     /locus_tag="PLP58_11920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ09272.1"
                     /translation="MKPLALLAILLLATAAFSMAPPMRRENAPQVSAMKAIETATAAL
                     ASKPGKYYCAKVILQEASMSPDPLFAARHWQLVFYEEGRERTVDAGGKETFGDIELNV
                     SAAGEIFGMRQLFSGPFPKNP"
     gene            complement(15798..16229)
                     /locus_tag="PLP58_11925"
     CDS             complement(15798..16229)
                     /locus_tag="PLP58_11925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ09273.1"
                     /translation="MKRRNLIRAILSIIALGFFTLGGAYIYHFRPLVSAAPWKARVTH
                     GTFGATDASVYRMLFSPNRLFIELPKTHRDRYWWFALDTDRKAVGVPNGPRREPYLHQ
                     NHDMNLGVGLQAQKIEDTWTVDWADRGVTFTNSNLKIELTQ"
     gene            complement(16378..>17043)
                     /locus_tag="PLP58_11930"
     CDS             complement(16378..>17043)
                     /locus_tag="PLP58_11930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ09274.1"
                     /translation="AIVCSVFVAVANDNQTKRELPGWGTAVNPDGDCTFFVADDALLI
                     SVPGSRPHDLAADINTTNAPRVLQSVRGDFTIQVMIEGRFAPGGDSTKAGRTGYNGAA
                     LVALFDDRNVVTLARAVLQRPGEDAMPYANFEIRVAGELQRIGLTGDHPLPKEGPIFL
                     RLERRGSKFLAGVSVDGISWDVLEPKQIPADWPKELQTGVAAISTSKDEFNPRFSKLQ
                     LLK"
BASE COUNT         3198 a         5657 c         5187 g         3001 t
ORIGIN      
        1 caggcggggc gggaccatgt ggtggcggag tacaatgaga actcgcgcgg ggaccggcac
       61 ccgatgcgcg ccatcatgac gcgcgagcac ggctacattt tcaatccatg gtcggacggc
      121 cggcgcgtga tgaccacggc cacgcagggc acggtgacct accggcggat gaaggcgctg
      181 gcggcctcgg acgcgggcat cgcggccagg ctgaacatga tagaccaccg cgcgccggag
      241 gagctttacc attacgccta tgacccggac gcgctcacga acctgatcgg ccgggcggac
      301 catgcggaga cgcaggcaag gctggcgcgg atgctggagg agtggatggt gcgcacgggg
      361 gatccgatgc tggaggtctt ccgccagcgc ggcgaccctg cggtgctcga cgcctacgtc
      421 acgcgccagc aggcggaggc ggaggcgcgc cgcgcgggga agaaaggcgg ggcccggggc
      481 gcaaaaaaag ccgccgccaa gggcaaatcc gccatgccgc cggagaatgc tgaaacgaaa
      541 aagggcgcgc gcggcagggg cgagacgggc ctcattgaga tcaggctgcc ggagtcggcg
      601 cggcgcggcg gggaaatgac ggcggtcatc ccgcactcgc tgcccgccgg gctgggggtg
      661 cggaagctgc atgtgacgct gaaggacgcc gccgggaaaa ggctggagcg caaggtcatc
      721 gagatcaccg gaaaggcgga ggcgcgggtg agcttcacac tgccgcccgg ttatgccggg
      781 gagagcgtga gcgtggcggc ctttgtcggc gaggagtatg agaagagcct ccagcacctc
      841 cagtcagacg cggtgccggt ggtcccttag gggtgtcagg ccgcgatcca gccggcgatg
      901 agggcggcga cttcgggatg gtcgaggtag ccgtagaatt tgtgcggatt ggcgccgtcg
      961 ctgccggtcc agaagttgta gatgcggcgg tctttgatgg actcgaggta gcccagcctg
     1021 cgcatttcct tgaagtcatc ggccacgccc tcgtcatggg agacgaagtc gtcatgggcg
     1081 gccacgtttt cccagcggcg gatgatgcgg cagggatatt tgccgtcagc gggctcgtgg
     1141 gcgtcataga ggtttttgat gacgccgggc tcgccgagcg ggcagccgag ggtgagccag
     1201 agggagacgc ggttgccggt gtcctggatg tccttgtact cgctcatctg ggagtatttc
     1261 cagagggtgt cataggagac gatgcagccc atgctgtggg agacgaggca gatgtcgtcg
     1321 ccggcgagga gggcgggctt gaggtggatg gccaggcgct ggcgcacctc ggagccggct
     1381 ttccgggaga gcaggtaggc gcccatgtcc ggggtggctt tggcgacgag ctggtcgttc
     1441 atgccgaaga tgccggccac ggcgctgagg acggaggcgg cctcgtcctt ccagcggcgg
     1501 tctttgtgct cgtcgagcag cttttggtag tcggcttttt tgaaggcggt ggttttgcgc
     1561 gccagcagct tgtccagatc gtcgtcataa ctgccgggaa gctcgcaggg gccggagccg
     1621 tatccggggt cgttgacgcc gggcaaatcg ctctttttct tctggccggc ggcgatcatg
     1681 aggcggttgt tgatgtcgcc gtagtaggcg tgggagcact cgacctttcc ggtctcgatt
     1741 tttttggcgg cgtcgccgtg ctcgcgcttg aggccgtgga ggagggcttt tttgacgagc
     1801 ctttctttct cgctgccgga gggtttggtg gagcgcccgt ggatgatgat gaggtgtttc
     1861 atggggaggg gaggcgggct ttgccgcgcg gcgcaaggaa agacgccggg gcccgcgccg
     1921 gcaagagcga atccccgcat tccgagtgtc acaaaattcg attcccgaat tttgattttc
     1981 gaactttgag gttcccactg gcattccaac ggctatgggt gcccccgtcg cccagtggcg
     2041 atcctttcaa acaaactcta tgtgcggcat tgtaggctac atcggcaggc ggcaggccag
     2101 ccccatcctt ctggacgggc tgcgccggct ggaatatcgc ggttatgact cggcgggcat
     2161 cgccctccag aacaacggca gcggcttgaa cttcatcaag cgcgcgggac gcattgacaa
     2221 tctgagcaag gcggtggccg aagccagccc ccagggcagc acgggcatct cccacacccg
     2281 ctgggccacc cacggcgcgc ccacggatga aaatgcccac ccccaccgcg accaaagcgg
     2341 caggctggcg ctcgtccaca acggcgtcat cgaaaactac cagacgctgc gcgaccagct
     2401 catcgcccag gggcacacct tcgaatccca gaccgacacc gaggtgctgg cccatctcgt
     2461 cgggcgctat tttgacgcct cggatgaaaa agacggcccc acccgcctgc gcaaggcgct
     2521 ccagtccgcg ctcgccaaag tcaagggcac ctacggcatc gccgccatgc atgcggacgt
     2581 gcccggcgtg cttgtcggcg cgcggctggg cagcccgctc gtggtcggcc tcggcgatga
     2641 ggagcatttt ctcgcgagtg atgtctccgc catcgtcgcc cacacgcgcg acgtggttta
     2701 cctcaacgac cacgatatcg tctccctcac cgagggccgc tacgatgtca cctccatggc
     2761 cgggggcgag gcgcaggtga aggtcagccg cgtcgagttc accgccgatg acgccgagaa
     2821 gggcgacttc ccccacttca tgctcaagga gatctacgag cagcccaagt ccctgcaaaa
     2881 cgccatgcgc ggccgccttt cccgcgacga ggccaccgcc atcctcggcg ggctgaacat
     2941 gaccccgcag gagctgcgcg gcgtggaccg catcatcctc agcggctgcg gcaccgcctt
     3001 tcacgcggcc atggtcggcg agtatctcat cgagaccctc gcgcgcgtgc ccaccgaggt
     3061 ggagatcgcc tccgagttcc gctaccgcaa cgtgcccgcg gacaggaaca ccctccagtt
     3121 cgtgctcagc cagagcggcg agaccattga caccctggcc gccctgcgcg aggcgcggcg
     3181 caagggcatc cgctgcctcg gcatcgtcaa cagcgtcggc agcaccatcg cccgcgagag
     3241 cgacggcggc tgctacatcc acgccggccc ggagatcggc gtcgccgcca ccaagacctt
     3301 cacctcccag gtgatgatca tgaacctgct cggcctgctc ttcggccgca ttcaccacct
     3361 ctccagtgcc gacggcatcg agatgattga cgagttggag gccatcccgg acaagatcac
     3421 cgccatcctc aagcagacgg acaagatcaa agccatcgcc gaaaagcacg ccaatgccga
     3481 gggcatgctc ttcatgggcc ggctgcaaaa ctaccccgtg gccatggagg gcgcgctgaa
     3541 gatgaaggag atcagctaca tctacgccag cggccatccc agcgccgagt tgaagcacgg
     3601 catcatcgcc ctggtgaagc ccgacatgcc ctccatcttc atcgcgccgg atgacgccgt
     3661 gtttgataaa aatgtcagca acatcgagga ggtgctcgcc cgcaaaggcc ccgtcatcgc
     3721 cgtcaccact gagggccgca ccgagctgga gcgcctcacc gaggacgtca tctatgtgcc
     3781 cgagtgcccc agctacctca gcccgctgct caccgtcatc cccatgcagc tcctggccta
     3841 ccacaccgcc gtcgcccgcg gctgcgatgt ggacaagccg cgcaacctgg cgaaaagcgt
     3901 gaccgtggag tgatggcggg gatcgcgctt gaaagttgaa cattccggct ttccaaagca
     3961 tcctcccctc gctcatccct ctcccctcgt gacacctctc cagaccctgc ccgtgctggg
     4021 cgcggcgctc cttgccgcgc tgcaaggcca cgcgcaggcc ccgcccgcga tgccggcgga
     4081 gcgttcaccc gacaccatcg ccgccggcgc cggggagttt gtggcgcggg cggttttctg
     4141 gacggaggac cagaccccgg cggatttcct cgatctcgcc ggccagcaca ccaaggcgcg
     4201 ctggcgcacc ctttaccgcc cgcccccgcc cggccccatg gccgaccgcg tgaaggccgc
     4261 gttcaccctc ggcagcctcg tgggggagac cttcctcatc tggcaggcgg gtgacgcgca
     4321 gcagttccgc aacaacaacc aggagctcgc caccttctgc cgcatcctcg gtctcggtga
     4381 aaaaatgcgc ccgcgcctca tggcccaggc gaagatggcc gagatggacg agtggaagga
     4441 catgcgccag gagatcgtgg acgggcacca ggagatcatg cgcactctgc gcgagcagat
     4501 ggatgacgac ctggccctgc tcgtggacat cggcgcctgg atgcgcgcgc tggagatcgt
     4561 ctccaagctc gtgcaggaga ccccggaagt ggacaagcgc cccctctgca tcggctcccc
     4621 cgccctgctc gccgacatgc gccaggactt cgccaagctc acccccgaaa accgccgctc
     4681 tccgcttctc cagcccgtca tcgccgtcat cagcgagctc gagcgcgtct ggggcgaccc
     4741 caaaatcggc gcccccaccc agaacatggt catccgcacc cacgagcagc tccgcgcggt
     4801 gatggaagag ctgactttga ggtgagtttt ccaggcggtt cgcggccgcg ggcgcatccg
     4861 actttagtcg gataaaaaaa gcgccgcgca tccgaccaaa gtcccatacc cgctccggcg
     4921 cggcgggtgc aggctgcggt tgtcatgaag acccaaacac acacccgcca ccgcatgaaa
     4981 cacaccacca cactcctcgc cgccgccctg ctcggccttg gcctccacca caccgccatg
     5041 gccggctcca acaacttcgc ctccgccacg gacctgacca cccacaactg gccccccgca
     5101 gtctcgctgg ccgccttcac cagcgagagc ggcgagcccg ggcaccgccc gaacggcagc
     5161 aatggagcgc agaaaagcgc ctggtggaaa tggaccgccc cgcacaacgg cttctgcacc
     5221 gtggacacga tgggccactc cgacgaccag ttcatttatg acaccctgat cgcggtttac
     5281 accggcacgg acgtcaacgc gctgacccgc gtggctggca acgacgacca tgacagccat
     5341 ctgggctacg gctcggcgaa ctcttccagc gtcacctttt acgccaccga gggcgccacc
     5401 taccacttcg ccgtggacgg ccgcagcgcc gccgccatca atgccagcaa ccacaaggca
     5461 cagctgcggc tgcgcctgtt gcgcgcccgg gccgagcacc gcgtcggcgt catcagcatc
     5521 ccattggagg agttcatgca cggcatgatc cagtttagca agacggcggg tcacaccttc
     5581 agcggcaaga tcctgatcaa cggcaagtcc caccccttca aaggcgtctt cggcctcgac
     5641 ggctactgct ccatctccat cgagcggatc gttcccagag gagccgcgcc ccagcctccc
     5701 ttgacgatcc tcctggacgg cacgcagagc attgccaacg gctcgccctt cacggagttc
     5761 gccctgatct cgcatttgat caacggcgcg tggtggggtt cgatggcgac gcggcagact
     5821 ttcgccaaag gcaccgagag cggactcaag gggcgctact cggcggccat cagcgccccg
     5881 gacaacgtct ggaaaagcgg agtcatgtcc ttcaccgcca ccggcgcggg agtcgtcaaa
     5941 ggcgccagcg tcctgccgga cggcaccaag accactttcg gcagcttcct ctgtgagaat
     6001 gacgcctcca gctcctgggt gcccgtttac aacggactct acaaaaacgc gggctacttc
     6061 gcctccaggc tgaagctcac cgaggcggga gccaccgacc aggtgacctc ggtgaatgtc
     6121 ttcgggcagc atcaccgccc cgaggctccc ggcagcctct tcatgccgga cgggaggaca
     6181 ttttttacca gcgtggtggt caaaggctcc acctacaccc cgccggcggc caaggcgcgc
     6241 gcgctcggct tcctcgacgg ctccatgggc gccggcaagc tcagcatccc catgtcctcc
     6301 ggcgagatca accccgccat catggaaaac ctcaccctcg acaccggcaa caaaatcatc
     6361 ttcgcctcca aagcccgcaa acccgccctc accctcaaca agtccaccgg cctcgtcacc
     6421 ggctacctcc tcgatgacgc cggaaaaaaa cgcagcctca ccggcgtgct cttcatggac
     6481 ggcatgacgc ccaagctgga gggccacgtc agcggcgcca ccaagaactc cttcttcgaa
     6541 gtcatcccct gatcctcgca caccccctcc ccctcaaggc tcccgcggca ccgcctgcgg
     6601 gagccttttc cttgggtggg gatcgaagtt cgaaacccgg agctcacgaa acccgtctcc
     6661 ttccgcgcgg ggcggatgga agcactcgca tggaaagaaa cccccggcgg cacgtgactg
     6721 cccggccccg gccatgtcag gcggcttgac atgtttcctc atcaccgcct tgaatgacag
     6781 gcggcgtgaa aaaaacaccc gccatgcctt atgaaacgga ccccgctcac gcatcgcctg
     6841 gccatgatga ttctggcagc ctcctcctcg tctgcttccg cgcaatccac cgccggcccc
     6901 gagcgtcaaa acctcctcgg ctgggccgaa ggcgccttca ccatccgcac cccgagccct
     6961 ccgcgcacgg aggcccatgt cgtggccctc gacggccagc cggccaccac caccatcggc
     7021 gtgccaaaaa acgcgcccct gccgcacgaa ttcgtgatcg aactgcccgc ctccaccacc
     7081 ttcaccagct tcgccgtgcc cgagattggc gagttcggcc ccgccaaggg caggcatgtc
     7141 aagaccgtcg agatcgcagg ctcgtcagaa agcgccgaaa gcggattctc acccctggcc
     7201 ggcttcgaga tccagatcga aaaaaaagcc ccgcaggaat tccccgttca ctcgccgcgc
     7261 cccgtgcgct ggctcaaagt ccgctttctg gaccgcttca cgccccagac cagcccggcg
     7321 gacccggtgc tcttttccga actcatgggc tacggcgccc aggagcagaa ggagcccccc
     7381 gaaaaaggct tcaacggtgt ctggaccctg cgcaggggct acgaagtctc gcgcaacctg
     7441 atcgagctgc gccaggatga aagcggccag gtcaccggct gccaggtgct cggcggccag
     7501 ctcagcaaaa tcagcggatc ggtggaagac ggcatcgtcc gctggatcag caccagcgtg
     7561 cagggcggca gaaatgtcag cgtcccctcc atcgccagga tcaccagcga aggcgagctg
     7621 cacggcgtct cctcctttca tggcggcctt cagacattct ccggcatccc cgccccgcaa
     7681 ggcgccacca ccccctgctc cgaggcgccc gaatctgaaa acgccgtcag cgccgccctc
     7741 caggccggac tcgccgccgt gctttacggc attcatttcg atgtggactc cgatgtcctg
     7801 cgcccggacg ccgcgcccgc cctggagcag atcctcgaag ccctggaaat gaacccctcc
     7861 atcgccgtca tcatcgaagg ccacaccgac tccgacggct cggacaccca caacctcgac
     7921 ctctccaagc gccgcgccgc ggcggtcgtc cgctggctcg ccggcagaaa ggtggacgcc
     7981 gcccgcctcg aagcctccgg caaaggcgaa agcacaccca ttgccgacaa cgcctccgcc
     8041 gtcgggcgcg ccatgaaccg gcgtgtcgaa gtgcgcccgc gctgaatccc ccagcctcca
     8101 tccagcttct ccgcatcacc ggcaatcccg ccgtcgcaga cgcgcagcgg gggatttttg
     8161 gacagaaccc tgaccggtcg aagcataagc tgacgccccg gaatgctccg ccgtccgatg
     8221 caggcggatg ccccggcatg tggaagcggc gtcccgccgc tcggcgggtg gagtggacgg
     8281 ggtgtgcgtt ttttatccca agcacggagt gggaagtcag acgtttttcc cacttctggg
     8341 ctggagaaaa cggggttttt caggtgcgcg gggaggtggc gaactggcac ggagacgacg
     8401 ggcttgtgtc aggcatggtt acctcccgca cttggcactc tgggagccgg tagccagctt
     8461 gtccgcgaac ccgccccccc ccatggctaa gcctccgcta ctgccgctgg accccttggg
     8521 tctcggggcg ggggttggaa aagtgtaact cctgttatga cctcaaaaca aaaacatcat
     8581 cgtctcaaac tggtgttgga gaaaacgcct ccaattctcg aagcttccaa acctgcctct
     8641 gatggcttag ccaagctcgt ttgggccatc ggtctgacca ctggctttat gattcttgct
     8701 tggaggctgc ccgaaactgc tcttgatgca ggcatcgtta cccgcgccat gaaatatgcg
     8761 cttcacaagc tcacccgcga atgacgtccg cagagccgca ttctcttaga catgctccct
     8821 cctcatcagc tcatccacct atgggacgtg aacaacgagg gccgcatcgc ctgattctca
     8881 ctctccctga acatgactcc cgaatgcatc cttctcatcg gcatccaggc ggcggggaag
     8941 tccacgttcg cggcggagcg ctttgcgcag acgcacgtca tcctgaacct ggacacgctg
     9001 cgcacgcgaa aacgcgagga gcggctgctg ttggagtgcc tggagcagcg gcgctcgttc
     9061 gtcgtggaca acacgaatgt ctcccgcgcc gagcgggcgc gctatctgcc gctggccggg
     9121 gaggcgggct accgccgcgt cggttatgcc ttcgaggccg ccatcggccc cgccctggaa
     9181 cgcaacgcgc gacgggagcg cgtggtgccg ccttatgccg tgcaagccac ccgcaaccgg
     9241 ctgcaatggc ccgctctcga tgagggtttt gacgaactgc attttgtccg ccttgccgga
     9301 aatggctttc gcatcgaacc ctgggaacct catgaaattt gacgatctgg accgcgagat
     9361 gcggcgcttt gagacagagc gggactggca ggtgccggag ggtgtcttca tcatcgcccg
     9421 catggacggg cgcggcttca cccggctgac caaggaggtg atgcgttttg aggcgccgta
     9481 tgacgagcgt ttccgcgacg ccatcgcggc caccgcgcgg catttgatga actgcggctt
     9541 ccgcgccctg cacgccttct gccagagcga tgagatcagc ctgctgctgc acccgcgcga
     9601 tggcacgttt caacgcaagg agcgcaaact gctctccgtg ctggccggcg aagccagcgc
     9661 cgccataact catgcgctgg gccggcccgc ctgcatggac agccggctct gcctgctgcc
     9721 ggactcggcg cgggtggcgg actatttccg ctggcgcatg gaggacgcgc ggcgcaactg
     9781 cctgaactcg cacgcctgct ggctgctgcg ccgcctggga cgggacgcca ctgccgctca
     9841 taatgagatt gaaggcctcg gtgtcgaaga gaaaatggcg ctgcttgccg ggcatggcat
     9901 cgtgtttgat gaactgcccg cctggcagcg gcgcggtttt gcggtcgagt ggcgggagga
     9961 ggaaaaagcg ggcatggacc cccgcacagg cactgcgacc gtgaccaccc gccggaggct
    10021 gcagacgatt ctggatctgc cggatggtgc ggaatatggg cagttgatcc tgactctttt
    10081 gcggagcgtg cccgatgacg aaagccggat gcatcatctg gcaggcacgc tgacgcactt
    10141 tcaggtgccc gcaccgattg aagacttcta tgatgacgga atgctgcggg tgcatatcga
    10201 gcgcaagcca ggcacgcgtt tggcgcacgg cttttgcttt ctccagcatc cctcggggcg
    10261 ccggtcatgg cagggttggt tcaaagaggg cgtacgctcc ggtcagtggg tgtattggaa
    10321 ggatacagat gaagcacatg tgggcgaggt gaccgaattc ggagatgctg aatacacgcc
    10381 tatgcatagc cgtccgcttg acggagcggc tctgtctcct gaaagagtca tcctgatcag
    10441 cgaggagtat ggatatcgct cctggtggtg gtggccggac cggcccatgg cagagatcat
    10501 ttgctggtgg aaggatctgc caactgtcga gcaatggtct ttgagcggcc ctgttttgcc
    10561 aggcagcaca attgaggttg ccgaagatga atcagccgcc ttccatcaga aatggatgga
    10621 tcatctgcat atacggcttc atctccacga ggatgacgac agtgtgctgc acctgcccgg
    10681 cggagagaca gtattgcacc aaggacaccc cgaagccgcg ctccaactgt aaagcaaaga
    10741 ccgccgcgca tcattgccgc ggacacctcg ttcccgctgt aaggggcgca ttttccgcta
    10801 tccactctcg cctcatgatg cactttccgg ccatgcgtgt ttccctggca gccgcccttg
    10861 tttttctccc gatcctttgc ctccgggcgc aggagcccgc gccggacgcg gagcctgaaa
    10921 ccgcgcccgc cctcaaagtc ccgcccaagg ccgccccgcc cgccgcaact gcgaaacctc
    10981 cgcccgagga tgcctaccgg cagatggagc tgctcacacg cgccatggag atgatccgcc
    11041 agaattatgt ggacgaggac aaagtcacct tcgccgaact cgtcgagggc gcgctggagg
    11101 gcatgctgcg ccgcctggac ccgcattgcg aatacatggg ccgcacgctg ttcgaggaca
    11161 tgcagcgcga gcacagcgac acctccgaag gcatcggcat caccgtggct ctgcgggaag
    11221 gcgcgctcac catcatcacc gtgcgcgagg acggccccgc cgggcgcgcc ggtgtgctgg
    11281 ccggagatca aatggtgcgc atcaatgacg tgctcaccga cagcgtcggc gtggccgagg
    11341 ccatccagct cctccagggc aaatccggcg aggcattgaa gctcaccatc cgccgccccg
    11401 gcacccggca gttcctcgag ttcagcatgg agcgcgaagt gctggccgag acctccgtgc
    11461 atgacgccat gctgctcacg ccaaaactcg ccggcggcgc caaagtcggt tacgcgcgca
    11521 tttcccagtt cacccagtcc accgcgcgcg acctctccgc cgcgctcgat gagctggagt
    11581 ccgccgacat gcaggccttt gtgctcgacc tgcgcaacaa ccccggcggc ctgcttgaca
    11641 gcgccgtcgc cgtctgcggc gagttcctgc cagcgggcac cgtcgtcgtc accaccgagg
    11701 gccgcatcgc ctcccaaaat cccccgccct accgcacccc cgcgcgcgac ggcaaaaaac
    11761 cccgccgcta ccccgtggcc gtgctcgtca atcacgccag cgcctccgcc tccgagatcg
    11821 tcgccggcgc cctccaggac ctgaagcgcg ccatcgtcgt cggcaccacc agctttggca
    11881 aaggctccgt gcaaagcatc ctgcccatga aaaacggcgc cgccatgcgc ctcaccaccg
    11941 ccaaatacta cacccccagc caccgcacca ttcacgagca cggcgtcacc ccggacatcc
    12001 tctccaacct cacccccgcc gaggagcagc gcgtggccaa gtggcgcacc cagcacggca
    12061 ccggcgaggc cgccgccatc gagatcgcca acctcggcga caaacagctc gagcgcgccg
    12121 tggacgccct caaaggcgtg ctcgtcttcg atgccttcgg ctccggcgag gccgccctcg
    12181 ccccccgcgc cctgcccgcc ggacagtgaa aaaaggccgc acccatgagg ccgcagcgca
    12241 tcctcatcct cgtcgaaaac ctgcccgtgc cgctcgaccg ccgcgtctgg caggaagcat
    12301 gtgctttgcg ggatgccggg cacgaagtca ccgtcatctg cccccggatg cgcggccaca
    12361 cccagcccga ggaaaagctc gacggcatcc agatctaccg ccactggatc accggcgagg
    12421 ccggcggcat caccggcttc ctcggcgaat acgccagcgc cctctgcggc gagttcctct
    12481 gcgccctcaa ggcctggcgg cgcaccggct tcgatgtcat ccacctctgc aacccgcccg
    12541 acctcatctt cctcgtcgcc ctgcccttca agctgctcgc cggcgtccgc gttatctatg
    12601 acgtgcatga cctctggccc gagatgttcg aggccaaatt cgcccgccga ggcctgctct
    12661 accgcgccgt ccgcctcgcc gagcgctgca ccctggccct ggcggacgcc gtcatcgcca
    12721 ccaacgaaag cgtgcgcgcc gtggtgaaaa agcgcgggcg caaaagcgat gacgaagtct
    12781 tcaccgtccg caccgcgccg gacaaaatgg acacctccgc gcccgaggac cccgcattga
    12841 aaaaaggccg cccccacctc gtcggctaca tcggcgtcat gggcagcgcc gatggtgtgg
    12901 actacctgct gcgcgccgcg catcacatcc tccacacccg ccagcggcag gacgtgcaat
    12961 tcctcctcat gggcggcggc cccgagcacg agcgcctgct cgccctgcgg gaagagctcg
    13021 gcctcgccgc ccatgtggac atgccaggcc gcgtcagcca ggcctttctc tgcaccgccc
    13081 tgcgcaccat ggacctcggc gccgcctgcg accccatcaa cgcctacaac gaccactgca
    13141 ccatgaacaa aaccctcgaa tacatggcct tcggcaaagc ccaggtcatg ttcggcatcc
    13201 acgagggccg cgaatccgcc ggcgaggccg cgctttacgt catggaaaac tcccccgaac
    13261 agctcggcga cgccatcctc gagctcctcg acgatccccc ccgccgccac cgcatgggcc
    13321 agctcggcca ccagcgcctg cacggcccgc tcagttggcc ccacagcgtc cgccagctcc
    13381 tccgcgctta tgcaagggcg ctggctggca aagcccctta gtttgtgcaa cgtcaaggat
    13441 gagcgacacg ccgccccaga ctccgcaacc agacaagacg tttacggcgt gttcgctcca
    13501 tccgtttgat ctgcctcgtg tggtcgctgt cagagcctct cacgccagta gtccggccgt
    13561 cttttgtggt gcatgacagc gacgatgcgg acgtgctgtc ctcgctcgtt gtagcggtag
    13621 aagatgtaat acggaaagcg tttgagccgg aagatgcgaa cgcctccgcc cacctgctgg
    13681 cagcggtcgg gagttttgag gatggctgcc acgccagcct caacagcctc ggtgaattcc
    13741 acacccaggc gctggcgtct ctcctcatac caggcacctg cattatcatt cttcgagtgc
    13801 gtcgggatga aactcgaacg tcatgactgt gaacgcctga gaacggactc acggacacga
    13861 cggagcgcct cctcgcctgg gacgccctga actgcgccag actccatctc gttagccctg
    13921 cgaatggcaa cggcgagctg tgtctcaaag acggacgggg cgtgcggcac actctcaagc
    13981 agccgctcag cgagcacaat ccggctctct ccggtcaggc aaagggcgtc ctcaaacaca
    14041 cggtcaacgg taattgtcat ggcgggaggg tagtgccagt cgaaaggaag gcaaacagcc
    14101 agtctggctc aagcccgcaa caggtgcttg ttggaagttg ggattgaaga tctgctcgct
    14161 gctcacggga gtgttgttga gcaggccgaa cgcttcggat caaacgacgg cgagcgggag
    14221 tcgttgctga cacgacagat agccttcgag ccgttgcctg catccgtgtt gttcggcgat
    14281 tagttgagtt tcattttttg cgcgcgacgt tacaaaccac cgttgttccg tgctgttcga
    14341 ggcggacatg aactccgggc tgatccgcag tggtggtgcc agacgcggga aagaaatcga
    14401 agcgccgaag gtctggaggg acatcgtaat gcgtgccaag tggcggcggc gcaaaggctg
    14461 tcgggtcgta gccaaccttt cgcatggctg tgtgagcgag agccaatgcg gttgcgtcgt
    14521 ctcggagggc gagcggcttt gctgctagac tgacggtgcg ccagcgatag tcccaaagag
    14581 tccatgaatt gaataaaacg taagccagaa gaagcgtccc caaaacgcca attgcaatgg
    14641 gtgctgtccg attggtgctc aatggccgtt gcatcgcttg ttgtggttag ccgaacgttt
    14701 cggatcaggc gacggcgagc gcaagacgtt gctgacacga cagatcgcct tcgagccgtt
    14761 gcctgcatcc gttttgttcg gcccttctgt tgctgtgttc attgcttctt ctgtccaaga
    14821 acagtttccg ggctgaccat cttgccatgc tcatcatata gcacgatgcg aagtccttca
    14881 ttcaggatcg cctttgccag aagctttcga ccttctgccg cccggggttt cagcacgttc
    14941 agagtcattc cggcgctgcc tactgcgaca ttctcgatct tgtgctcggt taggatggtg
    15001 tggactcttc ggccttcctt tgcgttcaat tccggtgtgt tcacccaccc agcgagggca
    15061 acaaactcag gatctcttgc tttgctgaat ggactgaagg cgaggacaac gacagcaacc
    15121 aacgggataa atcgcagtgt ggttttcatg attcgtagat tcttggagag catgacgatg
    15181 tggcacgctg tttcttggcc gaacgcttcg gatcaggcga cggcgagcgg aaagcgtcga
    15241 tgacacgaca gatgccatac gagccgttgc ctgcatccgt tttgttcggc gtcacggatt
    15301 cttcggaaaa ggcccggaaa atagctgccg catgccgaag atctctccgg ctgcgctcac
    15361 atttaattcg atgtcaccga atgtctcttt gccgcctgca tcaacggtcc gttcacgacc
    15421 ttcttcgtaa aatacgagtt gccaatggcg ggcggcaaat aggggatcag ggctcataga
    15481 tgcctcctga agaatgacct tcgcgcagta gtatttccca ggcttgctgg ctagcgctgc
    15541 tgtcgcggtt tcaatagctt tcatggcaga gacttgcggc gcgttctcgc gtcgcattgg
    15601 tggagccatc gaaaaagcag cagtagcgag aagaagaatc gcaaggaggg ctagtggttt
    15661 catgattttg gaagccttgt ggcgagtgcc gccgaacgtc tcggatcagg cgacggcgag
    15721 cgagaagcgt tgatgacacg acagatgcca ttcgagccgt tgcctgcatc cgttttgttc
    15781 ggcttgttga tggggggtca ttgggtcagt tcaatcttca agttgctgtt cgtgaatgtc
    15841 acacctcggt cagcccaatc aacagtccag gtgtcttcaa tcttctgggc ttgcaagcct
    15901 actccgaggt tcatgtcgtg attctggtgg aggtatggct ctcgtcgtgg gccgttcggg
    15961 acgccgacag ctttgcgatc ggtgtcgagt gcgaaccacc agtagcggtc tcgatgtgtt
    16021 ttcgggagtt caatgaagag tcgattcgga ctgaagagca ttcggtaaac gctcgcgtca
    16081 gttgctccga aagtgccgtg agtgacgcgg gctttccaag gcgcagcgct gacgagaggg
    16141 cgaaagtgat aaatgtatgc gccacccaag gtgaagaagc cgagcgcaat gatggagaga
    16201 atcgctcgaa taaggtttcg tctcttcatg gcgcttggtt cttagccgaa cgaccccaag
    16261 ctcagcgacg gcggagggct ggcgctgccg ctgcggcaag gcggcggggg gcgcaggcgg
    16321 tgggagcagc ggctgtgaca gcccggagcc gttcgctgca gcgcatggtt cggcgactta
    16381 cttgaggagt tggagcttcg agaaccgggg attgaattca tctttggagg tgctgatggc
    16441 tgcaacgccc gtttgaagtt ctttgggcca atctgccgga atttgctttg gttcgagaac
    16501 gtcccaactg attccgtcca cgctcacgcc agcaagaaac ttcgagccgc gtcgttcgag
    16561 acgaagaaat atcggccctt cttttgggag tgggtggtcg ccagtgagtc cgatgcgctg
    16621 gagttccccg gcaactcgga tctcgaagtt ggcatacggc atagcgtcct cgccggggcg
    16681 ttgaaggacg gcgcgtgcca aagtgacaac gttccggtcg tcgaatagcg caacgagggc
    16741 cgctccgttg tagcctgtcc gtccggcttt ggttgaatcg ccgccggggg caaagcgacc
    16801 ctcgatcatc acttgaattg tgaagtcgcc gcggacggat tgaagcaccc gcggcgcatt
    16861 tgtggtgttg atgtctgcgg ctaaatcgtg cgggcgtgaa cctgggacgc tgatgagtag
    16921 agcgtcgtcc gcaacgaaga atgtgcaatc gccgtctgga ttgaccgcag tgccccaacc
    16981 gggaagctcg cgcttggtct ggttatcgtt cgccactgcg acgaataccg agcagacgat
    17041 cgc
//
LOCUS       DAOMYJ010000103        16967 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Prosthecobacter sp. isolate AMR_MDS_5629
            NODE_1166_length_16967_cov_21.299018, whole genome shotgun
            sequence.
ACCESSION   DAOMYJ010000103 DAOMYJ010000000
VERSION     DAOMYJ010000103.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867850
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prosthecobacter sp. (wastewater metagenome)
  ORGANISM  Prosthecobacter sp.
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Verrucomicrobiaceae; Prosthecobacter.
REFERENCE   1  (bases 1 to 16967)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16967)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.30
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:34:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,742
            CDSs (total)                      :: 4,684
            Genes (coding)                    :: 4,676
            CDSs (with protein)               :: 4,676
            Genes (RNA)                       :: 58
            tRNAs                             :: 55
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16967
                     /organism="Prosthecobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1166_length_16967_cov_21.299018"
                     /isolate="AMR_MDS_5629"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1965333"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1437)
                     /locus_tag="PLP58_11935"
     CDS             complement(<1..1437)
                     /locus_tag="PLP58_11935"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HRJ09275.1"
                     /translation="MRTSLPSTQRWLAIRTQFVIDAGIVFFSVWLASAIRFEDLWPDA
                     MARYWPALVAGSLALPASVYILGLYSLQGAHLRKRERVALLCVAFVACLLVLLAMGSL
                     NFSSRIGRGVMAWQAPIGLALLLAHHGALVQRGLSARRRMAIVIGESRDELALRILQG
                     VSPPQTDVVGYFSPQPLGGALSTLHLGTCRDIVRQMDEREIDALICSDPHLRHPDMSS
                     ILRRVCFSGHPVLSLTDVLEEAYGAVPLSLVNMEWLVHASAMPRREYVRKLKRLFDVG
                     CSLVFGVVLALPLLAGMVLVRLTSKGPVFYSQVRSGRHGRTFKVHKLRTMRVDAEANG
                     AQWAAGRKDPRLTPVGGLLRTFRLDEIPQLWNVLTGDMSFVGPRPERPEFVEKLAQDI
                     PFFEERLLVQPGLTGWAQVCYPYGASVEDARRKLEYDLYYMKHMSMMLDFFILLDTVR
                     TVLRGGAARRGAEALKRIEQLAGEPEPEP"
     gene            complement(1584..1892)
                     /locus_tag="PLP58_11940"
     CDS             complement(1584..1892)
                     /locus_tag="PLP58_11940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ09276.1"
                     /translation="MSRLNVGLRLTNGWLRHPNDGIDPASAGTAGGPCARAGSHQKAW
                     GDAAATGTQLDAAIATRARSVAGVPQLKMTADSEYNPGQMQEIVHNSKALLQALQSGG
                     "
     gene            2214..3029
                     /locus_tag="PLP58_11945"
     CDS             2214..3029
                     /locus_tag="PLP58_11945"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF019846.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HRJ09277.1"
                     /translation="MKDLVENRRHITLTDKLRLALLIVRENGLLWCLCFAVYYVCSSV
                     SKRAFDRMDRLRREKGIPGMNSRALNKAIWEAWDWSAGGEEWSPSEEWKQAVMTDVLD
                     KHIPQGASVLEIGPGGGRWTGRLIGRAASFLGVDISESCVEVCSKKFGGKPGVAFMVG
                     SGCDLKGVPDASVGALWSFDVFVHINEPEVAAYADEFKRVFKPGAVGVIHHGSVGGKL
                     GGWRSNITHESMLELLKKRGFVIRESFKEWTTGGTTHQAGLYEDVITVFTLPA"
     gene            3026..3853
                     /locus_tag="PLP58_11950"
     CDS             3026..3853
                     /locus_tag="PLP58_11950"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014414.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HRJ09278.1"
                     /translation="MSAPATTRSPQALRHHYEVEKELAARLRASTREERTALFGKLYG
                     ELFERVPDHPRHTRKGSDQARERGVRAQMRLVGQVLKPGDVLVEFAPGDGWLSNAAAA
                     TAAEVIGIDISDQRDLSKPLPPNVRHVVYDGYHVDLPDACADVVFSYQFLEHLHPDDV
                     PAHFELARRLLKPGGCYVFDTPHRYSGPHDISRFFTDSLDCFHFQEWTHREMRRVLAA
                     HGFSTTWVFRMGRVQKGIFNLLVDALELLLLPLPHKLRRALCGRIFPSVALVAVRDS"
     gene            3850..4983
                     /locus_tag="PLP58_11955"
     CDS             3850..4983
                     /locus_tag="PLP58_11955"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HRJ09279.1"
                     /translation="MSAEKTSGGMLRVLHVLDSLAPGGLENGVVNVARRLHGRGFDIH
                     AACLRFRGDFAERMPEPDKVVVLGKTGGFSIKAVRALRAQMRATGADLLHSHNLGTLI
                     YAVAATLGGRVLPIVHGEHGQLQPQDLTRKRLWQRRLFFPLCRQVHVVSASVRDNLRD
                     LGLDPRRRILVTPNGVDSARFSPPEDKSAAKAALGLGAGDVVAGIVGRLVALKRHRLL
                     FDALSLLAAEMPALRLLVVGDGGAERDELIAAMKQHPHAERIRWMGHQNELEHCYRAM
                     DLLAAPSEIEGLSNAVLEAMSCAVPVLAHSACGNAEVIDPAVNGYLSDLRGAEDMASA
                     LRAALADPADLSKRGLAARRRVLERYSMEAMEDAYRRLYRGEC"
     gene            5072..8209
                     /locus_tag="PLP58_11960"
     CDS             5072..8209
                     /locus_tag="PLP58_11960"
                     /inference="COORDINATES: protein motif:HMM:NF037016.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HRJ09280.1"
                     /translation="MLLAGLIAQAGEVALDPSFGAGGVVLPSFPSLPSIEASGEGRCV
                     AVQADGKMVVAGHSLAGGRRILAVLRVLPDGTLDPAFGASGVALPFPSAADDQAACVR
                     LQPDGKILVAGRASAAGSSDMLVARLLADGSVDTSFATAGRLALDFDGQDDEAFALNF
                     DGSGRLLIAGTANDGGMLSAAVARCDADGAPDAAFGDQGRATFKILGAVENHGRDVVA
                     QAGGRIVLCGHASISNRDWFALYGFTSAGVADETFGTEGYQITAVGSGATIYSRAQSM
                     VLLGDGKMVLAGSGTVSGSERAALARYSEEGLLDETFGTGGSVTTSAGSGETSARCVA
                     LLTDGGLLVGGTLQGATSRFVALRYLPSGALDTAFGASGIRLAAFTGYEADARALAVD
                     ANGLTLAGTLAGDVVSSIGLARLTLGGNLDNSFSGDGRQEINLQRGQPISQARAVACQ
                     PDGKVVIAGSVATEEGFDVALLRRMPDGSADAGFGTAGRVILSLGPEDDFVWKMLLQT
                     DGRILVGGTTFDNGTEKAFVARFNSDGTLDAGFGTGGVVKDQAGPADSGIYALALQPD
                     GKIVGAGYAYNSGGTRFHFAAWRFTTAGALDTTFNSTGRHVSFVINNTNRDDFATAVA
                     VQADGKILTAGTAYTLADQSTANFGMIRFQASGALDSGFGSGGKVNTPFSGLTSQARS
                     VALQVDGKIVVGGYVESGGVGGAASFASSRYLAGGTLDGSFGTGGQAVVSLGSPYDQS
                     YDMVLDSEGRVLLAGTTFNGSTRIALLRLTTAGLPDQDFDGDGIAAISAGGDLLAYGM
                     AFQSDGRLLIAGAAGAGAMAARLTIAFDANTPPVAVDDTQFVLPGAGVAVEVLANDSD
                     ADNDALEILSFTQPASGGVVSQLGNSLFFAAALNFTGASFTYTVSDGAGGTDTAAVFM
                     TPVMTYAQWRVARFGAQANDPQIAGEDADPDFDGLDNLTEYALGRHPLQAETEAWATL
                     DVAAGRLVLTYMRWMAAVDVEVTPEFCTDLTGWDAQGVVVEELGDDGIMKTLRATGPL
                     PDLPGRQFGHLLITQ"
     gene            complement(8199..8963)
                     /locus_tag="PLP58_11965"
     CDS             complement(8199..8963)
                     /locus_tag="PLP58_11965"
                     /inference="COORDINATES: protein motif:HMM:NF015338.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HRJ09281.1"
                     /translation="MTCNRGQQGSQSQQGFKNSTRPDILVLQEAAGRSRGLAKSPENA
                     EFTHSASVGEHTILSRFPVLGSSILPAGAAPRQARAVRFVVNWFGRKISVYSVHLRTP
                     RDALRAQWPGALVYGVLGLPGTVWGERRKHYQAFWDGQIADAKMILEAAREDPLPVIL
                     AGDFNAPHTGHIRHLLTRELSDAHEEAGSGCGFTFPGVTRNPLSLGGPWLRIDYIFFN
                     HEWSALQCVTERDRPSQHRAVMARLVLRDDPPSVTE"
     gene            complement(9385..9603)
                     /locus_tag="PLP58_11970"
     CDS             complement(9385..9603)
                     /locus_tag="PLP58_11970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ09282.1"
                     /translation="MLAILTVFSMICTAIWLKPGPMVDNEDATWKADGGDSSRLTPGL
                     PVADDPDFLPEMSSDLEIRLEKSGKESP"
     gene            complement(9722..9880)
                     /locus_tag="PLP58_11975"
     CDS             complement(9722..9880)
                     /locus_tag="PLP58_11975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ09283.1"
                     /translation="MLLLLTTIAGLTGCSTYPFGHSHNGSYYGGQGSRTAPGTYVPKE
                     LRREHDLF"
     gene            10188..10487
                     /locus_tag="PLP58_11980"
     CDS             10188..10487
                     /locus_tag="PLP58_11980"
                     /inference="COORDINATES: protein motif:HMM:NF024788.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLDc N-terminal domain-containing protein"
                     /protein_id="HRJ09284.1"
                     /translation="MRTVIQRFFSFTAENVVPEIYAALGLVYLILLFVTLASVSKTQT
                     NGKLAWFMIVLFLPMLGMYFYAFWSLIKADYSFLTRLGFARSRSAPFREVQKSAP"
     gene            10521..12230
                     /locus_tag="PLP58_11985"
     CDS             10521..12230
                     /locus_tag="PLP58_11985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ09285.1"
                     /translation="MSHSLPSISRLKCPHHSLRPVLLALAGVWGLAHGQMIEPMLPVQ
                     PSQPEGFFPTLSDMSVLRGNDYPRSLTRGNMGEEGPDLRMQAGSGLFHYTGPEANFYQ
                     DYGAFFRPLDHVLGVFTPNDAWTLENQRVSNLSMSLDGRLGALTRRFSPENAHVKLGL
                     LYFDVLWFGGGVVYSDFHGGRNFEGDNEDGWTGYIDVAVRGLLRITDTIYVSAVADLM
                     YLPFENELALRFGNHNVPAVFFRINYGESFGPWDFLLYSEFRGVPGLDFYADADSPAI
                     DRAGRYYFGFNNVRTNEFQSDEQAYFSNRLGFKATRPVFDEQWRLGLEMDHTDFWRTF
                     GFEEHRKREHAAIWLGYEGSVLPFAPHLTYDIFSMDGYDSIWHRAYLRLNGRLTENLH
                     WTGMGGYRFTTGTTAEANRFIWDVGLDHTLTKNTRQFLNVGENFFNNEFLPETRTARY
                     ARYTLEQRIDKTFAIRAFAQYSDREQLNDELELRDRWGIGMSLIYQPLDFTSVRGTLM
                     YESIEKSGDFGDVDRWLYRLELQQQLGFRLTGRIFYQYEDRQGLNNDFKEHLTGLSMR
                     RYF"
     gene            12282..13190
                     /locus_tag="PLP58_11990"
     CDS             12282..13190
                     /locus_tag="PLP58_11990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ09286.1"
                     /translation="MKTVTHPRQNQRIQRQSRLLAGGLLLAAASIPFAADAQTYLALP
                     DKGVETQKDVPADFHKYFGQRINSWRDRQRQIAKLDLDADMNQDGVLSNTDPADSGSF
                     ESTPPGVVLGVGELTRMVIRLIPYRVDFDGEVVVTLEVEGINRGARTGEFETLDEEIA
                     SMGHVRVWRNKDKKDLLLDSRDPTKRVVEFTTDYKTYPYNLPLAVPRFFFVEGVNSSP
                     LYSGDVRILVTVSHRPPGQAPYAQTTSAKNVEPEVQAPPERVGIKSFRTSFDHVLMTV
                     TPNHVPKEFINGNNEGVWVAPPGKSY"
     gene            13435..14028
                     /locus_tag="PLP58_11995"
     CDS             13435..14028
                     /locus_tag="PLP58_11995"
                     /inference="COORDINATES: protein motif:HMM:NF014606.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis/export family
                     protein"
                     /protein_id="HRJ09287.1"
                     /translation="MIAQTQGEAPKPVNTMVFAPGDTLELFVKEDVTLNGSYQVREGG
                     YIVIPRAGRIQVAGLDRNMAEARVKEILEKTQLAVATVLVERTSKFPVSAPGTTPIAA
                     MPKVMVFITGAVPRSGVHHIPVNGTRPIGAYEALLITGGTGKFAREDRVEIYRTDAQG
                     KRQRAVFDLKAVRQGAAEDPPIGEGDIIHVPEKVFGF"
     gene            14140..16437
                     /locus_tag="PLP58_12000"
     CDS             14140..16437
                     /locus_tag="PLP58_12000"
                     /EC_number="2.7.10.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045226 - extracellular polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis tyrosine autokinase"
                     /protein_id="HRJ09288.1"
                     /translation="MNPRQSSKSQKGKLDSSKLIRLLLQFEDYWRLMALLFALGLLSG
                     AGYFVYARATFESTALIRINQFVDAAKAASGAQRDNNIALMRSLSKQLTSDFYQLEAA
                     KKLGVATASTTAVDLRASLIPAIRIGFLDQSHIQLRVISYRPRIVREFPQAMIEAYEG
                     ARKRLRNEYRDKAIARYVDELADVRAKVTSQLDSRLKFEEESALASAQIELERLSDVP
                     VQQVRVRYKLQEMDGVRQVLKDQGGTLDTVGTLALLTSMTSDKDDPLSVGRIVRRTGD
                     PRTSAPATFQTPEMAKEYNQIVVQPDMVEGIEPWRELDKKKRSLEEKMAQSRAKFLDD
                     HPEMIKLREELRETVSALELELQVARRAFDLEYEKLKEQLVELEAKLPDYYKAAKSYD
                     EKKLAYELMEKGQLAWDKAYEQLSRQIEGLEFGSDESAVSLEFRGFTDIRSEAPVSPN
                     KSKLLMLGCLLGLGMAGGVPFLLKQMDSTVTDLNEFEETLGIPGIGLVPLTDPEVLEE
                     VNRSQTVGSKVPNALLENFRLIRSSILLNRSPMGEARVIMLTSARPGEGKTTVSANVA
                     WAFSSMGEKTLLIDCDLRRGRVHNVAGVPNLPGLTRLLTGQATLEECLVKSPAENLWL
                     LPRGQVIAGTTELLNTEVFSQLLDSLRKQFSRIILDTPPVLGLSETAFLQNHADGVVL
                     VVRSASTPRSDVTAAFQQLEKLGGHFFGFVLNRVDFSKRSNHYDYYYYSSNYYDSNWE
                     EEEKEDREADERPGRKKRRGSSQAA"
     gene            complement(16453..16800)
                     /locus_tag="PLP58_12005"
     CDS             complement(16453..16800)
                     /locus_tag="PLP58_12005"
                     /inference="COORDINATES: protein motif:HMM:NF024248.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HRJ09289.1"
                     /translation="MSTPATPPTLPAAPPQVIPADTVYAALGEPTRRRILQILSDGQP
                     RTAGVLAGMVSKRLDATLKHLVGLRKAALVITADNTVDGRRQLYLLNPVIPVKPSAAG
                     GRELDFGYCLMRC"
BASE COUNT         3190 a         5069 c         5463 g         3245 t
ORIGIN      
        1 cggctccggc tccggctccc cggcgagctg ctcgatccgc ttgagcgcct ccgcgccccg
       61 gcgcgccgcg cctccgcgca gcaccgtgcg caccgtgtcc agcaggatga agaagtcgag
      121 catcatggac atgtgcttca tgtagtagag gtcatactcc agcttgcgcc gcgcgtcctc
      181 caccgacgcg ccgtacgggt agcagacctg cgcccagccc gtcagtccgg gctgcaccag
      241 caggcgctcc tcgaagaagg ggatgtcctg cgccagcttc tccacaaact ccggccgctc
      301 cgggcgcggc cccacgaagc tcatgtcacc cgtcagcaca ttccagagct gcgggatctc
      361 atccaggcgg aaggtgcgca gcagcccgcc caccggcgtc aggcgcgggt ccttgcgccc
      421 ggcggcccac tgcgcgccat tggcctccgc gtccacgcgc atggtgcgca gcttgtggac
      481 cttgaaagtg cgcccgtggc ggccgctgcg cacctgggag taaaagaccg gccccttcga
      541 agtcagccgc accagcacca tgcccgccag cagcggcagg gccagcacca ccccgaaaac
      601 cagcgagcag cccacatcga agaggcgctt gagtttgcgc acatactcgc ggcgcggcat
      661 cgcgctggca tggaccagcc actccatgtt caccaggctc agcggcaccg ccccgtaggc
      721 ctcctccagc acatcagtca ggctcagcac cgggtggccg gaaaagcaca ccctgcgcag
      781 gatggaggac atgtccgggt ggcgcagatg cgggtcggag cagatcagcg cgtcaatctc
      841 gcgctcgtcc atctgccgca caatgtcccg gcaggtgccc agatgcagcg tcgagagcgc
      901 gccgcccagc ggctgcgggc tgaagtagcc caccacatcc gtctgcggcg ggctcacccc
      961 ctggaggatc ctcagcgcca gctcgtccct gctctcgccg atcacgatcg ccatccgccg
     1021 ccgcgccgag aggccgcgct gcaccagcgc gccgtggtgc gccagcagca gcgccaggcc
     1081 gatgggcgcc tgccaggcca tcacccccct gccgatgcgc gaggaaaaat tcagcgaccc
     1141 catcgccagc agcaccagca ggcaggccac aaaagccacg cacaacagcg ccacccgctc
     1201 ccgcttgcgc aggtgcgccc cctgcagcga gtagaggccg aggatgtaaa ccgacgccgg
     1261 cagcgccagc gaccccgcca ccagcgccgg ccagtagcgc gccatcgcgt caggccagag
     1321 atcctcgaag cggatggccg aggccagcca gaccgagaaa aacacgatcc ccgcgtcaat
     1381 gacgaactgc gtgcggatgg ccagccatcg ctgcgtgctg gggagtgagg ttctcatgcg
     1441 tcgggagagt cgcggggggg aaatgctagc ggatgactcg caggatgcaa gccagggctg
     1501 ggaacgtttt tgatccccgg atcaagcagg ccgcgtgccg gagcgccccc tggcattgtc
     1561 aaccccgcgc gccgccgcgc cgctcacccg ccggattgca gcgcctggag cagcgctttg
     1621 gagttgtgga cgatttcctg catctggccc ggattgtatt cagaatcagc cgtcatcttc
     1681 agttgcggca cccccgccac gctgcgcgcc cgcgtggcga tggcggcatc aagctgcgtg
     1741 ccagtagccg ccgcgtcacc ccatgccttc tggtgggagc ctgctctggc gcatggtcct
     1801 ccagcagtac ctgcggatgc ggggtctatt ccatcgttcg gatgccgcag ccagccgttg
     1861 gtcagccgca acccaacatt caaacgcgac attacagcta tggtttgagg cagtattacc
     1921 ctacacctcc atcgaactgc ccgcctcatc cttcgagcta ccggctggcc tggcaccctt
     1981 catccggcaa gaccgtgaag taacgagccg agcagcccga cgcgggagga accttgggag
     2041 gctgcgcttt tcaagcggga cacgggctgt gttccgttgg ttgcctcatt tttgatcccg
     2101 ctttcctgga aatccagcag gcgcggtccg cgccgctggc gctgcccgtt tgaccgtccg
     2161 ccgcattcgt gctaggggcg gcgcttgcga ttcctgcaag ctccctctcc cgcatgaaag
     2221 acctcgtcga aaaccgccgc cacatcacgc tgaccgacaa attgcggctg gctctgctca
     2281 tcgtaaggga gaacggcctg ctgtggtgct tgtgcttcgc ggtttattat gtttgcagct
     2341 cggtctcgaa gcgcgctttt gaccggatgg accggctgcg gcgggagaag ggcattcccg
     2401 gcatgaacag ccgtgcgctg aacaaggcca tctgggaggc gtgggactgg agcgcgggcg
     2461 gggaggagtg gagcccctcg gaggagtgga agcaggcggt gatgacggac gtgctggaca
     2521 agcacatccc gcagggggcg tcggtgctgg agatcggccc cggcggcggg cgctggacgg
     2581 gccggttgat cgggcgcgcg gcctcttttc tgggcgtgga catttcagaa tcctgcgtgg
     2641 aggtgtgctc gaagaaattc ggcggcaagc ctggcgtggc gttcatggtc ggctcgggat
     2701 gcgatctgaa aggcgtgccg gatgcctcgg tgggcgcgct gtggagcttc gatgtcttcg
     2761 tccacatcaa cgagccggag gtggcggcct acgcggacga gttcaagcgc gtgttcaagc
     2821 cgggcgcggt gggggtgatc caccacggca gcgtgggcgg caagctgggc ggctggcgca
     2881 gcaacatcac gcacgagtcc atgctggaac tgctgaagaa gcgcggtttc gtcatcaggg
     2941 agtccttcaa ggagtggacc accggcggga cgacgcacca ggcgggcctc tatgaggatg
     3001 tgatcacggt tttcaccctg ccggcatgag cgcgcccgcc acgacacgca gtccgcaggc
     3061 gctgcggcac cactatgagg tggaaaagga gctggccgcc cggctgcgcg cctccactcg
     3121 cgaggagcgc acggctcttt ttggcaaact ctacggcgag ctttttgagc gtgtgccgga
     3181 ccacccgcgc cacacgcgca aggggtccga ccaggcgcgt gagcgcggcg tgcgggcgca
     3241 gatgcggctg gtggggcagg tgctcaagcc gggggatgtg ctggtggagt tcgcgcccgg
     3301 tgacggctgg ctgagcaatg cggcggcggc cacggcggcg gaggtcatcg gcattgacat
     3361 ctcggaccag cgtgacctga gcaaacccct gcctcccaac gtgcgccatg tggtgtatga
     3421 cggctaccat gtggacctgc cggacgcctg cgcggatgtg gtcttcagct accagttcct
     3481 ggagcatctg cacccggacg atgtgccggc gcatttcgag ctggcgcggc ggcttttgaa
     3541 gccgggcggc tgttatgtct tcgacacgcc gcaccggtat tcggggccgc atgacatctc
     3601 gcgctttttc accgactcgc tggactgctt tcacttccag gagtggacgc accgcgagat
     3661 gcgccgggtg ctggcggcgc acgggttttc cacgacatgg gtgttccgca tggggcgcgt
     3721 gcagaagggc atcttcaacc tgcttgtgga cgcgctggag ctgctgctcc tgccgctgcc
     3781 gcacaagctg cgccgcgcct tgtgcgggcg gatttttccc tcggtggcgc tggtggctgt
     3841 gagggactca tgagcgcgga aaaaacatcc ggcggcatgc tgcgcgtgct gcatgtgctc
     3901 gattcgctgg ctccaggcgg cctggaaaat ggcgtggtca atgtggcgcg caggctgcac
     3961 gggcggggtt ttgacattca cgcggcctgc ctgcgctttc gcggtgactt cgcggagcgg
     4021 atgccggagc cggataaagt ggtggtgctg ggcaagacgg gtggcttttc catcaaggcg
     4081 gtgcgggcgc tgcgtgcgca aatgcgcgcg acgggcgcgg acttgctgca cagccacaat
     4141 ctgggcacgc tgatctacgc ggtggcggcc acgctcggcg ggcgcgtgct gcccatcgtg
     4201 catggggagc acgggcagct ccagccgcag gatctgacgc ggaagcgcct ctggcagaga
     4261 cggctgtttt ttcccctctg ccggcaggtg catgtggtct cggccagcgt gcgggacaat
     4321 ctgcgcgatc tgggcctcga cccgcggcgg cgcatcctgg tgactccgaa cggggtggat
     4381 agcgcgcgct tttcgccgcc ggaggacaag tctgccgcga aggccgcgct cggcctgggc
     4441 gccggggatg tggtggcggg catcgtgggc aggctggtgg cattgaagcg gcaccggctg
     4501 ctttttgacg cgctgagcct gctggcggcg gagatgcccg cgctgcgcct gctggtggtg
     4561 ggggatggcg gcgcggagcg cgatgagctg atcgccgcca tgaagcagca ccctcatgcg
     4621 gagcgcattc ggtggatggg gcatcaaaac gagctggagc attgctaccg ggcgatggat
     4681 ttgctggcgg cgccctcgga gatcgagggc ctgtcgaacg cggtgcttga ggccatgtcc
     4741 tgcgcggtgc ctgtgctggc gcactcggcc tgcggcaacg cggaggtgat tgatccggcg
     4801 gtgaacggct atctttcgga tttgcgcggc gcggaggata tggcttcggc gctgcgggca
     4861 gctttggctg accctgcgga tttgtcaaag cgcggtcttg cagcccggcg gcgcgtgctg
     4921 gagcgctatt cgatggaggc aatggaggac gcttaccgcc ggttgtatcg aggggagtgc
     4981 tgagatcagg cgtgttttga cttttccgtc ctggcacgga cactgcaaag actcgaaagt
     5041 catgccgatg atccggactt tgtattcatc catgctgctc gcaggcctca tcgcgcaggc
     5101 cggcgaggtg gcgctggacc cctcctttgg cgcaggcggg gtggtgctgc cgtcctttcc
     5161 cagcctgcca tccatcgagg cttccggcga ggggcgctgc gtggcggtgc aggcggatgg
     5221 aaagatggtg gtggccgggc attccctggc cgggggcagg cgcatcctgg cggtgctgcg
     5281 ggtgctgccg gacgggacgc tggaccccgc gtttggcgcg agcggcgtgg cgctgccttt
     5341 cccatccgct gcggacgacc aggccgcgtg cgtgaggctc cagccggacg ggaaaatcct
     5401 cgtcgccggc agggcaagcg ccgccggcag cagcgacatg ctggtggcga ggcttctggc
     5461 ggatggctcg gtggacacat cctttgccac ggcaggcagg ctggcgctgg atttcgacgg
     5521 gcaggatgac gaggcctttg ccttgaactt cgacgggtca ggccgcctgt tgatcgccgg
     5581 gacggccaat gatggcggaa tgctctccgc cgctgtcgcg cgttgcgacg cggatggcgc
     5641 gcctgacgcc gccttcggag accaggggcg ggccaccttt aaaatcctcg gcgcggtgga
     5701 aaaccacggg cgcgatgtgg tggcgcaggc aggggggcgc attgtcttgt gcggtcatgc
     5761 cagcatttcg aatcgcgact ggttcgctct gtatggtttc accagcgccg gggtggcgga
     5821 tgagaccttc gggacggaag gctaccagat caccgccgtg ggcagcggcg ccacgattta
     5881 cagccgcgcc cagagcatgg tgctgctggg ggatggaaaa atggtgctgg cgggcagcgg
     5941 cacggtctcc ggaagcgaac gcgcggctct ggcgcgctac agcgaggagg gcctgctgga
     6001 tgaaactttc ggcacggggg gatctgtgac gacctccgcc ggttccggtg agaccagcgc
     6061 gcgctgcgtg gcgctgctga cggacggcgg cctgctggtc ggtggcacgc tgcagggcgc
     6121 cacatcccgg tttgtggcgc tgcgctattt gcccagcggc gcgctggaca ctgccttcgg
     6181 cgccagcggc atccggcttg cggctttcac cggctatgag gcggacgcgc gcgcgctggc
     6241 ggtggatgcg aacggcctga cactggcggg aacgctggcc ggtgatgttg tctccagcat
     6301 cggccttgcg cgcctgacac tggggggcaa tctggacaac agcttctccg gtgatggcag
     6361 gcaggagatc aatctgcagc gcggccagcc gatttcccag gcaagggcgg tggcatgtca
     6421 accggatggg aaggtcgtga tcgcgggcag cgtggccact gaggaggggt tcgatgtcgc
     6481 cctgctgcgc cggatgccgg atggcagcgc ggatgcgggt ttcggcacgg cggggagggt
     6541 gattttgtcc ctggggcccg aggatgattt tgtctggaag atgctgcttc agacggacgg
     6601 caggattctc gtcggcggca cgacttttga caacggcacg gagaaggcgt ttgtggcccg
     6661 cttcaacagc gacggcactt tggacgccgg cttcgggacc ggcggggtcg tgaaagacca
     6721 ggcgggaccg gcggactccg gcatttacgc gctggctctg cagcctgacg gaaagatcgt
     6781 cggggcaggg tatgcctaca attccggcgg cacgcgcttt catttcgcgg cgtggcgttt
     6841 cacgaccgcc ggcgcgctgg acacgacctt caacagcacg ggccggcatg tcagtttcgt
     6901 catcaacaac accaaccgcg atgattttgc gacagcggtg gctgtgcagg cggacgggaa
     6961 aattttaacc gcaggcactg cctatactct cgcggatcaa agcaccgcga attttgggat
     7021 gatccgcttc caagccagcg gggcgctgga ctcaggcttt ggctcggggg gcaaggtcaa
     7081 cacgcctttc agcggattga cctcgcaggc caggtccgtg gcgttgcagg tggatgggaa
     7141 aatcgtcgtc gggggctatg tcgagagcgg gggtgtcggc ggggcggcat cgtttgcgtc
     7201 gtcgaggtat ctggcgggcg gcaccctgga tggctctttc ggcaccggcg ggcaggcggt
     7261 ggtgtcactc ggcagcccct acgatcagtc gtatgacatg gtgctggact ccgaggggcg
     7321 ggtgctgctc gcgggcacca ctttcaacgg ctccacacgc atcgcgctgc tgcgcctgac
     7381 gacggcgggc ctgcctgatc aggattttga cggcgatggc attgccgcga tttcagcggg
     7441 aggcgatctt ttggcgtatg gcatggcgtt tcaatccgac ggcaggctgc tcatcgccgg
     7501 ggccgcgggg gcgggtgcga tggccgcgcg ccttaccatc gccttcgatg ccaacacacc
     7561 acccgtggcc gtggatgaca cgcagtttgt gctgcccggc gccggtgtgg ccgtcgaggt
     7621 gctcgccaat gacagcgacg cggacaatga cgcgctggag attctctctt tcacacagcc
     7681 tgcgtcgggc ggtgtcgtca gccagttggg gaacagtctg tttttcgccg cagcgctgaa
     7741 tttcacagga gccagtttca cctacacggt cagtgacggc gcgggcggca cggacacggc
     7801 ggcggttttc atgacgccag tgatgaccta cgcgcaatgg cgagtcgccc gcttcggcgc
     7861 gcaggcgaat gatccgcaaa tcgccggcga ggacgcggac cccgacttcg acggcctgga
     7921 taatctgacc gaatatgccc tgggcaggca tccgctacag gcggagacgg aggcgtgggc
     7981 gactctggat gtggcggctg gccgtctggt gctgacctac atgcgctgga tggcggctgt
     8041 ggatgtcgag gtgacgccgg agttttgcac ggatttgacg ggatgggatg cgcagggtgt
     8101 cgttgtggag gaattggggg atgacggcat catgaagaca ttgcgcgcca ccggccccct
     8161 gcctgatttg cctgggcggc agttcgggca tttgttgatc actcagtgac ggacggggga
     8221 tcgtcgcgca agacaagccg cgccatgacg gcgcgatgct gcgaggggcg gtcgcgctcg
     8281 gtaacgcatt ggagggcgct ccactcgtgg ttgaaaaaga tgtagtcaat gcgcagccac
     8341 ggcccgccca ggctgagcgg attgcgtgtc acgcctggaa aagtgaatcc gcagccgctt
     8401 cccgcctcct catgagcgtc actcagttcg cgcgtcagca aatggcggat gtgcccggtg
     8461 tggggcgcgt tgaagtcgcc tgcgaggatg acaggcagag ggtcctcacg agcagcttcg
     8521 aggatcattt tcgcatcggc aatctgacca tcccaaaagg cctggtaatg tttgcgccgt
     8581 tccccccaga cggttcccgg caagcccagc actccgtaaa ccagagctcc gggccattgc
     8641 gcgcgcaggg cgtcgcgagg cgtgcgcaga tggactgaat agacggagat tttccgccca
     8701 aaccagttca cgacaaaacg aaccgccctg gcctggcgcg gcgctgcgcc tgcgggcagg
     8761 atggaactcc ccagcaccgg aaagcggctc aaaatcgtgt gctctccaac gctggccgag
     8821 tgggtgaact cggcattttc cggggatttg gcgagtccgc gtgaacgccc cgccgcttct
     8881 tgcaggacca ggatgtctgg acgtgtcgag tttttaaagc cctgctgact ttgacttccc
     8941 tgctgccccc ggttgcaggt catgacgcgc aaaaccgccc cctcggagga ggatgttttg
     9001 tgaaactgcc agcctgaaag catcagggat gccgcaacgc cggcaaacat cgttccagcc
     9061 atccagagac gcccgaaatg gaatgtggac cacagcagga atggcagtgg aatgaaccac
     9121 aatgaaggcg gcgcatacaa tagcagggca gtggcgacat ttttttcacc ggcccacttc
     9181 agaacgagag gcagaagtat cagggcaacc aggtaaatta gaaggcatgt cctgccaatc
     9241 acgccgattt ttttgcggaa agtcgtcact gcttcgggag aaaagacggg ttcatttccc
     9301 ggtcatgcac agggagcgag ctcgccatat tttccaaagg ggcatgaacc tggatgcaac
     9361 cggatttttc ggtagctgtc ccgatcaagg ggattccttg ccactcttct caaggcgaat
     9421 ttccaaatcc gagctcattt caggaagaaa atcggggtca tctgcgacag gcagtcccgg
     9481 cgtgagtctg gaggagtctc caccatccgc cttccaggtc gcatcctcgt tgtcaaccat
     9541 aggcccaggc ttgagccata tggccgtgca gatcatgctg aatacggtga gtattgccag
     9601 catgagcaat gattccaaaa tagtgaggcc ggacgatgag aggcggctgc ttgacagccg
     9661 tcctgaggga aagcgcatga gagaaaaacc gggggagtct tacggcgtgt tgggagcgcg
     9721 ctcaaaagag atcatgctcg cgtcggagtt ccttgggaac ataggtgcca ggagcggtgc
     9781 ggctgccctg gccgccataa taactgccat tgtggctgtg cccgaaaggg taggtggagc
     9841 atccggtcaa gccggcgatt gtggtcagaa ggagtagcat gaagagtttc atgaaggtca
     9901 tgggagaacg aatgttgatc acaggcggag aatccgcgcc attttgcagg cgtattgtga
     9961 ggcattgggt cgcgaaatgc aagcctcatc tcccaagaca tcgcgaaccc gctcgtccac
    10021 gggctggttt gaaggagttt ggaaccgctc aagcgtgggt cacttggaag atgcgccgtc
    10081 agcggcggag ttgttgacag atgtgtctaa aaagaagcct aaattgagag agaaaatctt
    10141 ggacaacttc gcatcccgcg ccacatttga gcggcccatc ctcccgaatg cgcacagtca
    10201 tccaaagatt tttttcgttc accgctgaaa atgtggtgcc tgaaatctat gccgccctgg
    10261 ggttggtcta tctcatcctg ctttttgtca cgcttgctag tgtctcaaag acccaaacaa
    10321 acggcaaatt ggcatggttt atgatcgttc ttttcctgcc aatgctcgga atgtattttt
    10381 atgcgttctg gagcctcatc aaagccgatt actcattttt gacacgcctt ggtttcgcgc
    10441 gctccaggag cgcgcctttc agggaagttc aaaagtccgc gccctgacac gttgaatttg
    10501 agctgtattc ccaaactctc atgagccatt cactcccaag tatatcacga cttaaatgcc
    10561 cgcatcattc attacggccc gttttgcttg ccctggcggg tgtttggggg ctggcgcatg
    10621 gccagatgat tgagccgatg cttcctgtcc agccctcaca gccagaaggt tttttcccga
    10681 cactcagcga catgtccgtg ctgaggggca atgattaccc tcgaagcttg acgcggggca
    10741 acatggggga ggaggggccg gatctgcgca tgcaggctgg cagtggattg tttcattaca
    10801 ccgggcccga agccaacttt taccaagatt acggcgcatt tttccgtccc ctggatcatg
    10861 tgttgggagt cttcacgccc aatgacgcct ggacacttga gaatcagcga gtgagcaacc
    10921 tgtcaatgtc gctcgacgga cgtttgggtg ctctaacgcg ccgtttttcg cccgaaaacg
    10981 cgcacgtcaa actcggcctg ctttattttg atgtgctttg gtttggtgga ggcgtggtgt
    11041 attcggattt ccacggtggc cgcaattttg aaggtgataa tgaagacggt tggacggggt
    11101 acattgatgt ggcggtgcgc ggtttgcttc gcatcacaga caccatttat gtctcagccg
    11161 tggccgactt gatgtacctg ccgttcgaaa acgagttggc gttgcgtttc ggtaatcata
    11221 atgtacccgc cgtgtttttc aggatcaact acggcgagtc ttttggcccc tgggattttt
    11281 tgttgtacag cgagtttcgc ggagtccctg gactggattt ttacgcagac gcggattcac
    11341 cggcaattga tcgcgcaggc cggtattatt ttggatttaa taatgttagg acaaacgaat
    11401 ttcaaagtga tgagcaggca tatttttcaa atcgccttgg ttttaaggcc actcgccctg
    11461 tgttcgacga acagtggcgg ctgggattag agatggatca cacggacttc tggaggacgt
    11521 ttggctttga agaacatcgc aagcgcgagc atgcggcaat ctggcttggg tatgaagggt
    11581 ctgtcctgcc ctttgcccct catctcacat atgacatctt cagcatggat ggctacgata
    11641 gcatttggca ccgtgcatac ctgcgtttga acggtcggct cacggaaaac cttcattgga
    11701 ccggtatggg tggctaccgt ttcaccacag gaacaactgc agaggccaat cgtttcatat
    11761 gggatgtggg tcttgatcac acactcacca aaaacacccg tcagttcctg aatgttgggg
    11821 aaaatttctt caataacgag ttccttcctg aaacccgtac cgcacgttat gcgcgttaca
    11881 ctcttgagca gcgcatagac aaaacctttg ccattcgagc tttcgcacaa tattcagatc
    11941 gcgagcagtt gaacgatgaa ctggaattaa gagatcgctg gggtatcggg atgtcgttga
    12001 tttaccaacc gctggatttc accagtgtca ggggcaccct catgtatgaa agtattgaaa
    12061 aaagtgggga cttcggagat gtggatagat ggttgtacag gcttgaactc caacagcagc
    12121 ttggcttccg gctcactggg cggatattct accaatatga agaccgccag gggctgaaca
    12181 atgacttcaa agaacacttg accggacttt ccatgagaag gtacttttaa ttcgaatttc
    12241 cccgcatcat cactctcgct caccctccac acctaaaaac aatgaaaaca gtgactcacc
    12301 cgcgtcaaaa tcaacgaatc cagcggcaaa gccggcttct ggccggaggt ttgcttctgg
    12361 ctgctgcatc aattcctttt gcggcagacg ctcaaaccta cctcgccctg cccgacaagg
    12421 gcgtggaaac acaaaaagat gttcccgccg actttcacaa gtacttcggt caacggatta
    12481 attcctggcg tgatcgccag cgccagatcg ccaagctgga tctggatgcc gacatgaatc
    12541 aggacggcgt cctcagcaac accgaccccg cagatagcgg cagttttgag tccactcctc
    12601 ccggtgttgt gcttggagtt ggcgaactca cacgcatggt gatccgtttg atcccctatc
    12661 gagtggattt cgatggtgaa gttgttgtca cactcgaagt cgagggcatc aatcgcggtg
    12721 ccagaactgg cgagttcgag actttggacg aggaaatcgc gtccatgggt catgtccgtg
    12781 tttggagaaa caaggacaag aaggatttgc tgctggattc aagggacccg acaaagcgcg
    12841 tcgtcgaatt caccaccgac tacaaaacct acccgtacaa cctgcccctt gcggtgccgc
    12901 gcttcttttt tgtcgagggt gtaaattctt ccccgcttta ttccggggat gttcgcattc
    12961 tggtgactgt ttcgcatcgt cctcccggac aggcccctta cgcgcagacg accagcgcta
    13021 aaaatgtcga gcctgaggtt caggctccgc cggaaagagt gggcatcaaa agcttccgca
    13081 ccagctttga ccatgtgctc atgacagtca ctcccaatca tgtgcccaag gagtttatca
    13141 atggcaacaa tgaaggcgtg tgggttgccc ctcccggcaa aagttactga tacccggaga
    13201 caggtgagtc attcgcattg agtcaatcgc cctgatcgtg tgtttccgcc ggcttgctgg
    13261 cggttgacat ggatcggggc gattcttttc aatcaaaaca agcctcttcc ttccaaatcg
    13321 cagaacatgg gacagcattt cttcaaatcc ggcattcttg caacggtcat accctgtgtg
    13381 atagggggat gctcgaactc cccgcaggag caggatcccc tgccgcctcc ggcgttgatc
    13441 gcccagacgc agggagaggc gccgaagcca gtgaacacaa tggtgttcgc tccgggggac
    13501 actctcgagc tttttgtcaa agaggatgtc acgctcaatg gcagttatca ggtgcgcgag
    13561 ggcgggtaca ttgtcattcc gagagcggga cgaatccagg tcgccgggct tgatcggaac
    13621 atggcggagg caagggtgaa ggaaattctc gaaaaaaccc agctcgcggt ggcgacagtg
    13681 cttgtcgaac ggacttcgaa gttcccggtt tccgcgccag gcaccacgcc gatagccgcc
    13741 atgcccaagg tcatggtttt catcacgggt gctgtgccca ggtccggtgt tcatcatatt
    13801 ccggtcaacg gcacacgacc cattggcgcc tacgaagcgc tattgatcac gggtggtacc
    13861 ggcaaattcg cgcgcgagga tagggtggaa atctatcgaa ccgacgcgca ggggaaacgc
    13921 cagcgcgcgg tgttcgacct caaagcggtc aggcagggcg ctgccgaaga ccctccgatc
    13981 ggggagggcg acatcattca tgtgccggaa aaagtcttcg gtttttgaga tgccgcatga
    14041 ctttggcggc gcgggcatca tccgggatga tccgcgcgcc agcctcaata aagagagcgt
    14101 catgcttgaa aacgtcccga tgaaccgctt tgatgtttca tgaaccccag gcagtcctcc
    14161 aaatctcaaa aaggcaagct cgactcctcg aagttgatcc gcctcctgct tcagtttgag
    14221 gattactggc gcttgatggc gctcctcttc gcccttgggc tgctttccgg cgccggttac
    14281 tttgtgtatg cgcgcgccac cttcgagtcc accgcgctga ttcgaatcaa ccagtttgtt
    14341 gacgcggcaa aagcggcctc gggcgcgcag agggacaata acattgccct gatgcgctcc
    14401 ctgtcgaagc aactgacctc ggatttctac caactggagg cggccaaaaa gcttggcgtg
    14461 gccaccgcct cgaccacggc ggtggacttg cgggccagct tgatccccgc catccgaatc
    14521 gggtttctcg accagagtca catccagctt cgcgtcatct cctacaggcc gcgcatcgtg
    14581 cgcgagtttc cgcaggccat gatcgaggcc tacgagggcg cgcgcaagcg cctgcgcaac
    14641 gaatacaggg acaaagccat cgcccgttat gtggacgagc tggcggatgt ccgcgccaaa
    14701 gtcacctcgc agttggactc gcgtctgaaa ttcgaggagg agagcgccct ggccagcgcg
    14761 cagatcgagc ttgagcgcct cagtgacgtg ccggtgcagc aggtgcgcgt gcgctacaaa
    14821 ctccaggaga tggacggcgt gcgccaggtg ctcaaggacc agggcggcac tttggacacc
    14881 gtgggcacac tggcccttct gaccagcatg acctccgaca aggacgaccc gctgagcgtg
    14941 gggcgcatcg tccgccgcac gggcgacccc aggacatcgg cgccggccac attccaaaca
    15001 ccggagatgg ccaaggagta caaccagatc gtcgtccagc ccgatatggt cgagggcatc
    15061 gagccctggc gcgagctgga caagaaaaaa aggtcccttg aggaaaaaat ggcgcaaagc
    15121 cgcgccaagt tcctcgacga ccatccggag atgatcaagc tgcgcgagga gctcagggag
    15181 accgtctcgg cgctggaact ggagttgcag gtggcccgca gggcgtttga tctggagtat
    15241 gaaaagctca aggagcagtt ggtcgagctg gaggcgaagc tgccggacta ctacaaggcg
    15301 gccaaaagct acgacgaaaa gaagctggcc tacgagttga tggagaaggg ccagctcgcc
    15361 tgggacaagg cctacgagca gttgtcccgg cagatcgagg gcctggagtt cggatccgac
    15421 gagtccgccg tgagcctcga gttccgcggc ttcaccgaca tccgcagcga ggcgcccgtc
    15481 tcgcccaaca aatccaagct gctcatgctc ggctgcctgc tcgggctggg gatggcgggc
    15541 ggcgtgccct tcctgctcaa gcagatggac agcacggtca cggacttgaa tgagttcgag
    15601 gagaccctgg gcatccccgg catcggcctg gtgccgttga cggacccgga ggttctggag
    15661 gaggtgaacc gctcccagac cgtcggctcc aaggtgccca acgccctgct ggaaaacttc
    15721 cgccttatcc gcagcagcat ccttttgaac cgcagcccca tgggcgaggc gcgcgtcatc
    15781 atgctcacca gcgcgcgccc cggcgagggc aagaccaccg tctccgccaa cgtggcctgg
    15841 gccttctcct ccatgggcga gaagaccctg ctcattgact gcgacttgcg acgcggccgc
    15901 gtccacaacg ttgccggcgt gcccaacctg cccgggctca cccgcctgct caccggccag
    15961 gccacgctgg aggagtgcct ggtcaagtcc cccgccgaaa acctctggct gctgccgcgc
    16021 gggcaggtca tcgccggcac gaccgagctg ctgaacacgg aggtcttctc ccagttgctc
    16081 gacagcctgc gcaagcagtt ctccagaatc atcctggaca cgccgcccgt gctcggcctg
    16141 agcgagacgg ccttcctgca aaaccacgcc gacggcgtcg tgctcgtcgt caggtccgcc
    16201 tccaccccgc gcagcgatgt caccgccgcc ttccagcagt tggaaaagct cggcggccac
    16261 ttcttcggct tcgtgctcaa cagggtggac ttctccaagc gcagcaacca ctacgactac
    16321 tactactact cctcgaacta ctacgactcc aactgggagg aggaggagaa agaggaccgg
    16381 gaagccgacg agcgccccgg gcggaaaaag cgccgcggca gcagccaggc ggcgtgaaac
    16441 cggccccgcc tctcagcagc gcatcaggca gtagccaaag tccagttcgc ggcccccggc
    16501 ggccgagggc ttcacaggga tcaccgggtt cagcaggtaa agctggcggc gaccgtccac
    16561 cgtgttgtcg gcggtgatca ccagcgccgc cttccgcagc cccaccaagt gcttcagcgt
    16621 ggcgtccagc cgcttgctca ccatccccgc cagcaccccc gccgtccgcg gctggccgtc
    16681 cgagaggatc tgcaaaatcc tccggcgcgt cggctcgccc agcgccgcat acaccgtgtc
    16741 ggccgggatg acctgcggcg gagccgccgg cagggtgggg ggcgtggcag gagtgctcat
    16801 ggcgggattt tgaccgaacc ggccgcccct ggcaagtccc gcgcaacatt cgcaggccgg
    16861 gcgcgggctc catccaccgg gaaaaacccc gccgcccgtc caggagcaga ttccgaaccc
    16921 gcgccaaaga cctttccgcc accccggcag cctgccctgc ctccgcg
//
LOCUS       DAOMYJ010000104        16879 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Prosthecobacter sp. isolate AMR_MDS_5629
            NODE_1176_length_16879_cov_22.357466, whole genome shotgun
            sequence.
ACCESSION   DAOMYJ010000104 DAOMYJ010000000
VERSION     DAOMYJ010000104.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867850
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prosthecobacter sp. (wastewater metagenome)
  ORGANISM  Prosthecobacter sp.
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Verrucomicrobiaceae; Prosthecobacter.
REFERENCE   1  (bases 1 to 16879)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16879)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.30
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:34:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,742
            CDSs (total)                      :: 4,684
            Genes (coding)                    :: 4,676
            CDSs (with protein)               :: 4,676
            Genes (RNA)                       :: 58
            tRNAs                             :: 55
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16879
                     /organism="Prosthecobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1176_length_16879_cov_22.357466"
                     /isolate="AMR_MDS_5629"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1965333"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..223
                     /locus_tag="PLP58_12010"
     CDS             <1..223
                     /locus_tag="PLP58_12010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ09290.1"
                     /translation="NQCHRSTDKLRDALLPGLQRFWQDAQAVLRWVGRPWQHDQANAS
                     CPRQNPIESSGWYRRPPEWWIEASSRQKT"
     gene            429..749
                     /locus_tag="PLP58_12015"
     CDS             429..749
                     /locus_tag="PLP58_12015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ09291.1"
                     /translation="MGTEALVETRIFTAGIESNFPPPRSPIVEDEFALPLDAHQNDIM
                     IIPQPQPWPNQAKATLSRAAVFTGGWLPLKDSNLEQQSQKAVRCELEAFYRKAFSGKS
                     GSLD"
     gene            complement(760..4719)
                     /locus_tag="PLP58_12020"
     CDS             complement(760..4719)
                     /locus_tag="PLP58_12020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ09292.1"
                     /translation="MPKRQWPASPSEAWQRFKRLVGRVVSTPVGLVVRIKPGHFGSLV
                     SKKPLDGKPKGFVYGFNSPAQGRTALESGSVNDEQVAGYEPERAAMVESVPEILSAPD
                     VVLRPKTDSAKLLFFKGSVPGTSNSRVVVTMDTKTGELGFLSVHPKNIKEGFLKQHDI
                     VYMRPKNNGNAAPDGGSASTPVSKAPSAASQTPSQDGPKQAAPQQESGDNAAAGGPGA
                     SSVKEWAKTTFGQRLRSDGRLRESWRQTIGGQYRVESEAEWQARANTFIEEHGLEGAF
                     ALMMDPDSGLSPSDQVALGLQLILSTDAAIRAAERAGDAARVEMLDELLYETSEHVEL
                     LGTRLGQGVRVFGMWSRMSAEGVLGTFERKVDEAREKDMAVKVGGKPEDIAAEVTAAA
                     AEELADTTAEALGETTSEQLADMHRQLDELRRQLAEATAAAEAARHEAATAPTPAEAA
                     KAEQDAQRAEKTRQDLQRKVKRAEARRETKAEQAGKPRPPREKKPKPVKAEEIAKNWI
                     ERLARIHSDTQGWGSGQHVNAMTQLIRDYIKGENPDVMSDFAGMAGALGVPETMAEQL
                     QRQLDTERKAIAAVARERAILRLVAQMTPRLATKETKARIPRFVAKLLDAHELGALSR
                     PDFLDAYAEAFELPRFDAEMVKRVRALIDRQRAAPEGFLKQQATTDLMGELAKFRGIG
                     AMDIGTAFWYANILSGLSTQAINLWGNATHLMLKALTIGATHSPRESWQFLRGLFDGA
                     RRGGLEAMAALRDGTVPYRGDLKFSQGQVLELIHSDSPQTWGQRWKNGLALGRFVFRA
                     LAAGDALFYHTAREGRAHLEAARYASTRQRLEGGSFADYLAEQLNHGPERFAQAMEQA
                     RAELEAAGKPALHRALERRAWEILEQQRPPDLNEEALRFGAVTTFTQEPEGTMGAVAA
                     LINDAHRRLSLPSPWGPVRVLTPFIPFVNIVANVTSMAMDYTPVGILRGALGRHARHV
                     LDRSQKDFTPWEAKQRLMSGILGTAGTGLAFAWAYAMREQDDDEVPFMIYGMGPGTKA
                     RREQMPQGWKPFTVKVGGSYWSYSETPLALVFAVAGSTLDYLRYNPNGREERALGAAS
                     YALATSPQALLKTGVLSSVNDLFDLIEGNRDGKQFAARFASGFIPGQALLRDVSELIH
                     GDKIDDASLAAALLKDVPIIRPLVGKPALNVFGEPVQLDTLQRLPIMKRIVTGQGDDP
                     DARWLAQNKLWVAGLDKELALGKYLAEPDKARLKNADWRAERVRTLGRAAGDVLTEDE
                     KHAFVKAAGPRIRRAVREMKGLQQAYPQMSTEQMQNQLNAKIVAQRRLAMRGVLGLE"
     gene            complement(5021..5422)
                     /locus_tag="PLP58_12025"
     CDS             complement(5021..5422)
                     /locus_tag="PLP58_12025"
                     /inference="COORDINATES: protein motif:HMM:NF012974.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DnaB-like helicase N-terminal domain-containing
                     protein"
                     /protein_id="HRJ09293.1"
                     /translation="MFPQAPDAEEALLGAMLLDMPGVFDAHGRRLKDELFHNELNRLL
                     AHAMVDLHVSALPVDMVSVTNWLRKDGQLERVGGPARVAGCFLALSSSMGAAMAGYYL
                     DILEGMAARRMLLRHGDGGATDASGGGCRAD"
     gene            complement(5433..6380)
                     /locus_tag="PLP58_12030"
     CDS             complement(5433..6380)
                     /locus_tag="PLP58_12030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ09294.1"
                     /translation="MNYLNLNLIELRDAAFAAATDVERGVWLGLMLHCTAMENGGVMA
                     DAADWSERKWMMTCGVSLPLLADQDSELWWWEGDDLHVWGYPHEAQNRVNAGREGGKK
                     GGRPRKKNPPENPPENPPENPPENPKGKEWKEKEGKEKGKGMEGRSESGPAENILLAL
                     EVDEASPDEPDQLEWSGDQGWQGLTDTLSAELSAAYPACDLKRQFAAMDQWLKSNPAK
                     ARKSNWRRFATNWLSREQDRGGDLRGSNPLPAKPGFGLEKKEGGAAAADPDWDWRELH
                     VEVYGAPAAGRWGELAKSVRFELRQAWEQKNGRGGAADA"
     gene            complement(6377..6640)
                     /locus_tag="PLP58_12035"
     CDS             complement(6377..6640)
                     /locus_tag="PLP58_12035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ09295.1"
                     /translation="MSEELTSKAKLPPRLAVLVCEARPEGLYPLTRYDLREDGHMEVA
                     AGRSKAALGLQVLQDAVAVCFKTCGELAGEQAIKRLLEAEKRA"
     gene            complement(6633..6890)
                     /locus_tag="PLP58_12040"
     CDS             complement(6633..6890)
                     /locus_tag="PLP58_12040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ09296.1"
                     /translation="MIPMTIGLLTAGALGALGMWLGWNMGRALGLEMLERAARTERQA
                     LALVELCRKLREQADLALAEAIVERARDDEGEEWKGGADRE"
     gene            complement(6907..7203)
                     /locus_tag="PLP58_12045"
     CDS             complement(6907..7203)
                     /locus_tag="PLP58_12045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ09297.1"
                     /translation="MSRSEMALVARRTAEAGELLHGIREAAGTLQARLTKLRGMATPE
                     PLDEAKVKILEALACVNRAELLVNLPGECPATVPRSGAVWLAAGAVGGRGTGEF"
     gene            complement(7203..8273)
                     /locus_tag="PLP58_12050"
     CDS             complement(7203..8273)
                     /locus_tag="PLP58_12050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ09298.1"
                     /translation="MNELMGIYEPETDYRAEKLKREIAEAKKKGLILMAGQPAPAEAG
                     AAERAPEALAAQGHLRMSFEEVTPTKAEFWLRQNKGNRRLKRDVVEGYARQMRRGEWM
                     LLPPGIVFDVNGTLLNGQHTLNAVIKAGVTVRMVVTRGVPVALEGKADVRAASVMDSG
                     VPRNAADQMTIQHGVAAGASVIGAMVRSIVNAMLPRRTRRLAVSEIMTVYRMFEAELK
                     WMAENRSKERGLKQAGVMAAFTLAQRWPGESTGALVREMWVILVNDAGYLRARPECAA
                     LAHLHEWLTSECGLLMTRRDDRALLPLTLQVLRCCLGGDGVTDASSLERIRETGEALE
                     TLLPSAETSEVRKLFGVEGGAA"
     gene            complement(8266..9054)
                     /locus_tag="PLP58_12055"
     CDS             complement(8266..9054)
                     /locus_tag="PLP58_12055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ09299.1"
                     /translation="MAAPKNHPRWWSERFVAGLHRLHMKGVPCKTVAGACGVRNAASV
                     RQAFLARGLPVRVWESGHFQATHKPRTWVQVLGIIGRQTRMCVPAELRYDWRKFWTLA
                     QKQKFLREMRAHLGIPELVVRLEGARVFDAACPVARQMMAEVNGGLPSRLHRMRLKAA
                     TRGVLFEGQLWFWAEKHGFVNGARTKTLYHHIYERYHAPVPPGMRVRFLDGDPMHLEP
                     GNLYLASADEVCRENHAKALERTAAKRMTAMLNLQKTKGGPNHE"
     gene            complement(9054..10454)
                     /locus_tag="PLP58_12060"
     CDS             complement(9054..10454)
                     /locus_tag="PLP58_12060"
                     /inference="COORDINATES: protein motif:HMM:NF012372.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA cytosine methyltransferase"
                     /protein_id="HRJ09300.1"
                     /translation="MKARNIKAADLFCGAGGTSTGAQMAADACGVRLELTAINHWDVA
                     IETHSANHPGARHLCADVNDVRPETFFKRGELDWLFASPECTHFSKARGGLPVDDQRR
                     CGARRVLDWAERIYPARIWVENVQEFLDWGPLDVKGRPIVKHRGALFQEWVTCLRTLG
                     YRVEWRIQNAANFGAPTTRQRLIVQAARRGTRLVWPDATHRKPDATGDLFTELPAWLS
                     ARDHVIDWSIPCPSIFGRKKPLSNNTLRRIEAGLRAQGIEAFLVQSNFGSDARPNRVR
                     GIDEPMGTVVGGQSWGLAQPFIVTLRGTGANAGYTARPVGEPLSTVACCGSHHALITP
                     CLIPQQSDGRARPVTEPVPTVSTAGAIGLMQPMLVEYYGTGTHASCDEPLNTVTCKPR
                     FGLAVPLEGGGEGLLDIGYRMLRWRENARAQSFPDSYEFRGRTEEMIHKQIGNAVPPK
                     LAEAHARAALMERRAA"
     gene            complement(10451..11602)
                     /locus_tag="PLP58_12065"
     CDS             complement(10451..11602)
                     /locus_tag="PLP58_12065"
                     /inference="COORDINATES: protein motif:HMM:NF019146.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage Gp37/Gp68 family protein"
                     /protein_id="HRJ09301.1"
                     /translation="MAENSKIEWCDHTFNPWRGCTKVSPGCAHCYAETLSRRNPAVLG
                     EWGKGKPRVLASNDMWRQPLKWAVAAQNSRVLNGAIWRAEANVTTWDNRDIVERTVLI
                     KLATSSRRELTRELPLSEWEALPCPRPRVFCASLADWLDDEVPIEWLARLLELIFSTP
                     HLDWLLLTKRPQNWAGRILEVEKMARQSYCGEPRTTPDFDWKDAGAFYEWITDWTTGN
                     YIPANVWIGTSVEDQARADERIPRLLSIPAKVRFLSCEPLLGPVDLHSPHIKVTTWRV
                     GEYFDGPPPLNGIHWVIVGGESGPGARPMHPDWARSLRDQCEAASVPFFFKQWGEWSS
                     GNLADGPVFLGESGEFEDSPRRRVGKKRAGRLLEGREWNEFPNLEGGGR"
     gene            complement(11595..12458)
                     /locus_tag="PLP58_12070"
     CDS             complement(11595..12458)
                     /locus_tag="PLP58_12070"
                     /inference="COORDINATES: protein motif:HMM:NF014342.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DHHA1 domain-containing protein"
                     /protein_id="HRJ09302.1"
                     /translation="MKTTPIFVLYHANCPDGFAAAWAAWMTIGNAATYMPVNYGEPLP
                     DLPDGAMVIMVDFSYPRPILEALAERCQVTVLDHHPTAQEALRGLDFATFDLQKSGAV
                     LAWEHFHGTDVPEMLLYIQDRDLWQWKLPASREINAGLWKGTPREFGAWKTIHALWHS
                     GVTTAKERLIQAGEILATYDALTVDQLCKHPHWMRVLNWTVPAVASPVLQSEIGHRLL
                     ELHPTAPFAAIYMTLDDGTFAYSLRARHDDFDVSVVAKEFKGGGHKAAAGFRAIDRFT
                     VHAKKEGREDG"
     gene            complement(12548..12865)
                     /locus_tag="PLP58_12075"
     CDS             complement(12548..12865)
                     /locus_tag="PLP58_12075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ09303.1"
                     /translation="MRTDTFQSGNGALSAQLKDPAESRDVREIEGLLCFQREQLTELE
                     ALYETLRGRLADVCCPVPEAPKPKSETPLSSTVLGNLINNTNERLMTLNQRLQFLLEE
                     LQL"
     gene            complement(13019..13432)
                     /locus_tag="PLP58_12080"
     CDS             complement(13019..13432)
                     /locus_tag="PLP58_12080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ09304.1"
                     /translation="MNTRYSITLTGSGHSGDEPNSIEQRLRKLLQQLDSNGHSIETAS
                     LLVGGKQVALKIHDRTIPVPLEKESQSVPDTANPALHVNTLLREVHEGVMTLLERTAP
                     KPTKQPARKATEKKEGPPPAESVDPGNDSGSFTDF"
     gene            complement(13647..14249)
                     /locus_tag="PLP58_12085"
     CDS             complement(13647..14249)
                     /locus_tag="PLP58_12085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ09305.1"
                     /translation="MSEEITTELGFAVSVAETEEARGRWPDLTRLEQRRPDILGAIVW
                     FLARGVSVKTITRALKVSPCTVNRVRHDPKWKVAVVSESNGVVQKLDEILALTVDEVL
                     AQAREGNLPSIFDLKLLFDIRQLLSGGVTQRVEVKISADEEEFARFLAQARALPSPPN
                     GMVFDAEFLPQSRDVTPAETPDRANTKEGQTDLESVSNGQ"
     gene            complement(14821..15270)
                     /locus_tag="PLP58_12090"
     CDS             complement(14821..15270)
                     /locus_tag="PLP58_12090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ09306.1"
                     /translation="MNSKPLSEAIVNAASLLLTEGSKGRCGYWALREGHIHAKVPAGV
                     KVPEWMHFVIFEHVDWNDDLMKPSVSTVACFSGCEPALARARQMLVCLRAAEQGRTGK
                     VSVKFSLVVELHLAGGDMGACGWLNCETLEVTIFDHAKPKLEEVLEA"
     gene            complement(15473..15712)
                     /locus_tag="PLP58_12095"
     CDS             complement(15473..15712)
                     /locus_tag="PLP58_12095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ09307.1"
                     /translation="MNTIAEIFRPEELRTARTEAIDETLKEMRTFARKAPDPAKRIAL
                     SELEKLLRDTRALWVTAGLVNPEPEGARGDAAMAA"
     gene            15881..16207
                     /locus_tag="PLP58_12100"
     CDS             15881..16207
                     /locus_tag="PLP58_12100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ09308.1"
                     /translation="MNRAVVSFHLTGARPIRDDHLSAYCRCLDAEEKARLVSAWLRDA
                     LAPEDAATVLDPGTHRLQEEVRAWRPGLTDRQKSQLEFWASRLVADPDLAEIFDIITR
                     RAGWQG"
     gene            complement(16243..16395)
                     /locus_tag="PLP58_12105"
     CDS             complement(16243..16395)
                     /locus_tag="PLP58_12105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ09309.1"
                     /translation="MPPTIYPGRQEPKRSLFDVGWDFCYNALGFIVHITAWTLPLWLL
                     TKCVGG"
     gene            complement(16467..>16879)
                     /locus_tag="PLP58_12110"
     CDS             complement(16467..>16879)
                     /locus_tag="PLP58_12110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ09310.1"
                     /translation="VDLRRRLVRHDAAQELHAPRGLGGATPVPSGRSMKDHPERRLET
                     AARRGNLHAAAALAGIPMAAGGVMESFLDGPFQGFQNGRVFRLTNGTLWEQQEMKSSL
                     GGGLVNKVMILQTSEGTFMQLDGSGERVRVRQVR"
BASE COUNT         2919 a         6057 c         4593 g         3310 t
ORIGIN      
        1 caatcaatgc caccgcagca ccgacaagct gcgcgacgcg ctgctgccgg gcttgcaacg
       61 cttctggcag gacgctcaag ccgttctcag atgggttggt cgcccctggc agcatgatca
      121 agcaaacgct tcgtgtccgc gccaaaatcc gattgaatcg tcaggatggt atcggaggcc
      181 gcccgagtgg tggatagagg cctccagtcg tcagaagacg tgacgttcat tgctgacgat
      241 ccgggcgacg catgcatcgc ggtcttggat ccgtatcgct ccccgcccga ttacgggctt
      301 ttcgcagtat gggtagcgcc tgagcattgc gtccgaggcg ttggagacgc cctgatcgcg
      361 tcagtgatcg cacaggcgcg gaggcaggca gccggcagac tggtgcttca gatagccatg
      421 cataactttt gggcacggaa gctttggttg agacacggat tttcacggct ggaatcgagt
      481 ccaattttcc accgccacgc tcgcctattg tcgaggacga gtttgcgctg ccattggatg
      541 cccatcagaa tgacatcatg atcattccac agccacaacc atggcctaat caagccaagg
      601 cgacactgag tcgagcggcg gttttcaccg gcgggtggct acccctcaag gactcgaacc
      661 ttgaacaaca gagtcagaag gccgtgcgct gtgagttgga agccttttat cgaaaagctt
      721 tcagcggaaa atcaggctcg ctagactgaa tgctagacat cactcaagcc ccaaaacccc
      781 tcgcatcgcc agccgccttt gcgccacgat cttcgcgttg agctggttct gcatttgctc
      841 ggtgctcatc tgcgggtagg cctgctgcag gcctttcatc tcgcgcaccg cgcgccggat
      901 gcgcggcccg gcggctttca caaaggcgtg cttctcgtct tcggtcagca cgtctcccgc
      961 cgcccggccc agggtgcgca cgcgctcagc gcgccagtcg gcgttcttga ggcgcgcctt
     1021 gtccggctcg gccaggtatt tgcccagcgc cagttccttg tcgagcccgg cgacccagag
     1081 cttgttctgc gccagccagc gcgcgtccgg gtcatctccc tggccggtca cgatgcgctt
     1141 catgatcggc agccgctgca gcgtgtcgag ctgcacaggc tcgccaaaaa cattcagcgc
     1201 gggcttgccc accagcggac ggatgattgg cacgtctttc agcagcgccg ccgccagtga
     1261 ggcgtcatct atcttgtcgc cgtggatcag ctccgacaca tcgcgcagca gcgcctggcc
     1321 tggaatgaaa ccactggcaa agcgagcggc gaattgtttg ccgtcccggt taccttcgat
     1381 gaggtcaaag agatcgttca cgctgctcag cacgcccgtc ttcagcagcg cctgtggcga
     1441 tgtggccagc gcgtagctgg ccgctcccag cgcgcgttcc tcgcggccgt tcgggttgta
     1501 gcgcagatag tccagcgtgc tgcctgccac ggcaaagacc agcgccagcg gcgtctcgga
     1561 gtagctccaa tagctgccgc caaccttcac cgtgaaaggc ttccaccctt gcggcatctg
     1621 ctcgcggcgc gccttcgttc cgggccccat gccgtagatc atgaacggca cctcgtcatc
     1681 atcctgctct cgcatggcat acgcccaggc gaatgcgagt cccgttcccg cagttcccag
     1741 gatgccgctc atgagccgtt gcttggcctc ccacggcgtg aaatctttct ggctcctgtc
     1801 cagcacatgc cgcgcgtgcc tgcccagcgc gccgcgcagg atgcccaccg gggtgtaatc
     1861 catcgccatg cttgtcacgt tcgccacgat gttgacgaag ggaatgaagg gcgtcaacac
     1921 cctcaccggc ccccaaggcg agggcaggga aagccgccgg tgcgcgtcat tgatgagcgc
     1981 cgccaccgct cccattgtgc cctccggttc ctgcgtgaat gtggtcaccg cgccgaaccg
     2041 cagcgcctcc tcattcaggt ccggcggccg ctgctgctcc aggatctccc acgcccgccg
     2101 ttccagcgcg cgatgcagcg ccggcttgcc tgccgcctcc agctccgcgc gcgcctgctc
     2161 catcgcctgg gcaaagcgct ccggcccgtg gttgagctgc tcagccaggt agtcggcaaa
     2221 gcttccaccc tccagccgtt ggcgagtgct cgcgtagcgg gccgcctcca gatgcgcgcg
     2281 cccctcccgc gctgtgtggt aaaacaaagc atcccccgcc gccagcgcgc ggaagacgaa
     2341 gcgccccagg gccagcccgt tcttccagcg ctggccccat gtctgcgggc tgtcgctgtg
     2401 gatcagctcc agcacctggc cctggctgaa tttcagatct ccacggtagg gcaccgtccc
     2461 gtccctcaga gccgccatgg cctccagtcc gccgcgccgc gccccgtcga agagaccgcg
     2521 caggaattgc cagctctccc ttggggagtg cgtggcaccg atggtgagcg ctttcagcat
     2581 caggtgcgtg gcgttgcccc acaggttgat ggcctgcgtg ctcagcccgc tcaggatgtt
     2641 ggcataccaa aaagccgtgc cgatgtccat ggcaccgatg ccccggaact tcgccagctc
     2701 tcccatcaga tccgtggtgg cctgctgctt caggaagccc tccggcgccg cccgctgccg
     2761 gtcaatcagc gcgcgcaccc gcttcaccat ctccgcgtca aagcgcggca gctcgaaggc
     2821 ttccgcgtag gcgtccagga aatccggccg gctcagggcg ccgagttcgt gcgcgtccag
     2881 cagcttcgcc acaaagcgcg ggatgcgcgc cttcgtctcc ttcgtcgcca gcctcggcgt
     2941 catctgcgcc accagcctca ggatggctcg ttcccgtgcc actgccgcga ttgccttgcg
     3001 ctcggtgtca agctggcgct ggagctgctc agccatcgtt tccggcactc ccagcgcgcc
     3061 ggccatgccg gcaaaatcgc tcatcacatc cgggttttca cccttgatgt agtcgcggat
     3121 cagttgcgtc atcgcgttca catgctggcc gctgccccag ccttgcgtgt cgctatggat
     3181 gcgcgccagc cgctcgatcc agttctttgc gatctcctcc gccttcaccg gcttgggctt
     3241 cttctcgcgc ggcgggcgcg gcttgcccgc ctgttccgcc ttcgtctcgc gcctggcctc
     3301 cgcgcgcttc actttgcgct gcagatcctg gcgcgtcttc tccgcgcgtt gcgcgtcctg
     3361 ttccgccttt gccgcctccg cgggtgtggg ggcggtggca gcctcatgcc gcgccgcctc
     3421 cgctgccgct gtcgcctcgg ccagttgcct gcgcagctcg tcgagctggc gatgcatgtc
     3481 cgccagttgc tcgctcgtcg tctcacccag ggcctcggca gtggtgtcag ccagctcttc
     3541 ggcggcggca gcggtcacct cggcggcgat gtcttccggc ttgccgccca ccttcacggc
     3601 catgtccttc tcccgcgcct cgtccacctt gcgttcgaag gtgcccagca caccctccgc
     3661 gctcatccgg ctccacatgc caaagaccct cacaccctgc cccaggcgcg tgcccagcag
     3721 ctccacgtgt tcgctcgtct catagagcag ctcgtcgagc atctccacgc gcgccgcgtc
     3781 tcctgcgcgc tcagccgccc ggatcgccgc gtccgtcgag aggatgagct gcaggcccag
     3841 cgccacttga tcactcgggc tcagtccgct gtccgggtcc atcatcaggg cgaatgcacc
     3901 ctccagcccg tgctcctcaa tgaaggtgtt cgcccgcgcc tgccactccg cctcgctctc
     3961 cacacggtat tgcccgccga tggtctggcg ccagctctcc cgcagtcgcc cgtcactgcg
     4021 cagccgctgg ccgaaggtgg tcttcgccca ctcctttacg gagctggcac cgggaccgcc
     4081 ggcggcggcg ttgtcgccgg attcctgttg cggggccgcc tgtttaggcc catcttggct
     4141 tggagtctgt gaggcggcgg atggcgcttt actcaccggc gtgcttgcag agccgccatc
     4201 cggcgctgca ttcccattat tctttgggcg catgtaaaca atgtcatgct gcttcagaaa
     4261 tccctctttg atgttcttgg gatggacact gagaaaccca agctcacctg tcttggtatc
     4321 catcgtgacg acaacacggg agtttgaagt gccaggtact gaacccttga agaacagcag
     4381 cttggccgaa tcggtctttg gccgcagaac cacatccggc gcggaaagga tttccggcac
     4441 actctccacc atcgcggctc gctcgggctc ataaccggcc acttgctcgt cattgacact
     4501 gccagactcc agcgctgtcc gcccctgtgc gggagaattg aaaccataaa caaaaccttt
     4561 gggtttgcca tccagaggct tctttgaaac cagtgagccg aagtgcccgg gcttgatcct
     4621 caccaccaaa cctaccggtg tgctcaccac cctgcccacc agacgcttga atctttgcca
     4681 cgcctcactc gggctggctg gccattgacg tttgggcaag cttgattccg tcaccaaatc
     4741 catcacagat tgcatcacgc cagcatcagc gctgtccgcc accaccggaa gtgtgtctgg
     4801 cgcatttcca ggtttcgcgt ttgctgcttc ggcctctttg ctggcattct catccgcagt
     4861 ctccaccacg aaaatgtctg tctcactggc atgggtggac agcgtttgtc tagctgcctg
     4921 ctcattggcc ggatcaaacg gcactgccgc aacttggctg ccatcatccc gcctcagcac
     4981 aagcattggc tgcgcgggag aggacggctc ctgcgcagcc tcagtcggcg cggcatccac
     5041 ccccggacgc atcagtcgcc ccgccgtcgc catgccgcag cagcatccgc cgcgccgcca
     5101 tcccctccag gatgtccaag taataccctg ccatcgccgc gcccatcgag gatgacagcg
     5161 ccaaaaaaca ccccgccaca cgcgccggcc cgccaaccct ctccaactgc ccgtccttcc
     5221 tcagccagtt cgttaccgac accatgtcca ccggcagcgc cgacacatgc agatccacca
     5281 tcgcatgagc cagcagtcgg ttcagttcgt tgtgaaacaa ctcatccttc agccgcctcc
     5341 cgtgcgcgtc aaacacgcca ggcatgtcca gcagcatggc acccagcagc gcttcctcag
     5401 catccggcgc ctgcggaaac atggcagaaa catcaggcat ccgccgcgcc cccccttcca
     5461 tttttttgtt cccaagcctg cctcagctca aagcgcaccg acttcgccaa ctcaccccag
     5521 cgccccgccg ccggtgcccc gtaaacctcg acatgcagct cccgccaatc ccaatcagga
     5581 tccgccgccg cagcgccccc ttcttttttt tccagcccaa aaccgggctt cgcaggcaac
     5641 ggattcgaac cgcgcaaatc tccgcccctg tcctgctcac ggctcagcca gttcgtcgcg
     5701 aaccgccgcc agttgctctt gcgcgccttc gccgggttgc tcttcagcca ttgatccatc
     5761 gccgcaaact gccgtttcaa gtcacacgcc ggatacgccg cggacaactc agcgctcagc
     5821 gtgtccgtca gaccctgcca gccttgatca cccgaccatt caagctgatc gggttcatct
     5881 ggggaagctt catccacctc cagcgccagc aaaatattct ccgccgggcc ggattcactc
     5941 cggccttcca ttccttttcc tttttctttt ccttcctttt ctttccattc ctttccttta
     6001 gggttttcgg gagggttttc gggagggttt tcgggagggt tttcgggagg gtttttcttc
     6061 cttggtcggc ctcctttttt gccaccttcc cggcctgcat tgacacgatt ttgcgcctca
     6121 tgaggatacc cccagacatg cagatcatcc ccctcccacc accacaactc tgaatcctgg
     6181 tccgccagca aaggcagact cacaccacaa gtcatcatcc attttcgctc ggaccaatcc
     6241 gccgcgtccg ccatcactcc gccgttctcc atcgccgtgc aatgcagcat cagcccgagc
     6301 cacaccccgc gttccacgtc cgtcgccgcc gcaaaggcgg catcgcgcag ctcgatcaag
     6361 ttcaggttca gataattcat gctcgttttt cagcctccaa aagtcgtttg atcgcctgct
     6421 cacctgcgag ctcgccgcag gttttgaagc acaccgccac agcatcctgc agcacttgca
     6481 gtcccagagc cgccttggac ctgcccgctg ccacctccat gtgcccatcc tcgcgcagat
     6541 cgtagcgcgt cagtgggtaa agaccctccg gccgcgcttc gcacaccagc accgccagcc
     6601 gcggcggcag cttcgccttg cttgtcagtt cttcactcac ggtcggcacc tcctttccat
     6661 tcctcgccct cgtcatcccg cgcgcgctcg acaatggcct ccgccagcgc cagatccgcc
     6721 tgctcccgca gcttgcggca aagctctacc aaagccagag cctgcctttc cgtgcgcgca
     6781 gcccgctcaa gcatttccag acccagggca cggcccatgt tccagcccag ccacatcccc
     6841 agcgccccca gggcgccggc cgtcaaaagt ccaatcgtca tcgggatcat gtcagtgtgt
     6901 gttgtttcaa aattcacccg tgcccctgcc gcccacggct ccagccgcca gccaaacagc
     6961 cccggaccgg ggcacggtcg ccgggcactc acccggcaaa ttcaccagca gctccgcgcg
     7021 gttcacgcac gccagagcct ccaaaatttt cactttcgcc tcatcgagag gctccggcgt
     7081 cgccatgccg cgcagctttg tcagccgcgc ctgcagagtg cccgccgcct cgcggatgcc
     7141 atgcagcagc tcgccggcct ccgccgtccg ccgcgccacc aacgccattt cactccggct
     7201 catcacgccg cccccccttc caccccgaaa agtttccgca cctcggaggt ctccgcagac
     7261 ggcagcagcg tctccagcgc ctcgcccgtc tcgcggatcc tctccagact gctcgcatcc
     7321 gtcaccccgt ccccgcccag gcagcagcgc agcacctgca gcgtcagcgg cagcagcgcc
     7381 cggtcatccc gccgcgtcat cagcagcccg cactcggagg tcagccactc gtgcaaatgc
     7441 gccagagccg cgcactcagg ccgcgcgcgc agatacccgg catcattgac caggatcacc
     7501 cacatctcac gcaccagcgc ccccgtgctt tcccccggcc accgctgcgc cagcgtaaag
     7561 gccgccatca ccccggcctg cttcagaccg cgctccttgc tccggttctc cgccatccac
     7621 tttagctcgg cttcgaacat ccggtaaacc gtcatgatct cgctcaccgc cagccgccgc
     7681 gtccgccgcg gcagcatcgc attcacgatg ctgcgcacca tcgcaccgat cacgctcgcc
     7741 ccggccgcaa cgccgtgctg gatcgtcatc tggtccgccg cattcctcgg cacgcccgag
     7801 tccatcacgc tcgccgcgcg cacgtccgcc ttgccttcca gcgccacagg cacgccgcgc
     7861 gtcaccacca ttcgcacggt gactcccgcc ttgatcaccg cgttcagcgt gtgctgcccg
     7921 ttcagcagcg tcccattcac gtcaaacacg atgcccggcg gcagcaacat ccactccccg
     7981 cgccgcatct gccgcgcgta gccttccacc acatcccgct tcagccgccg gttgcccttg
     8041 ttctgtctca gccagaactc agccttcgtc ggcgtcacct cctcaaaact catgcgcaga
     8101 tgcccctgcg ccgccagcgc ttccggcgcc cgttctgccg cccctgcctc cgctggcgcg
     8161 ggctgtccgg ccatcaggat caagcccttc ttcttcgcct ccgcgatctc ccgcttcagc
     8221 ttctccgcgc ggtagtccgt ctccggttca tagatcccca tcagttcatt catggttcgg
     8281 gcctcctttc gttttctgta gattcagcat cgccgtcatc ctctttgccg ccgtccgctc
     8341 cagggccttc gcatgattct cccggcatac ctcatccgcg ctcgccaagt aaagattccc
     8401 cggctccagg tgcatcgggt caccgtccag gaacctcacc cgcattcccg gcggcaccgg
     8461 cgcgtgatac cgttcataga tatggtggta cagagtcttc gtgcgcgcgc cgttcacaaa
     8521 gccgtgcttc tctgcccaga accaaagctg cccctcgaag agcaccccgc gcgtggccgc
     8581 cttgagccgc atcctgtgca accggcttgg cagcccgcca ttcacctcgg ccatcatctg
     8641 ccgtgccact gggcaggccg cgtcaaacac ccgcgccccc tccagcctca ccaccagctc
     8701 cgggatgccc aggtgtgcgc gcatctcgcg caaaaacttc tgcttctgcg ccagcgtcca
     8761 gaatttacgc cagtcataac gcagttccgc cggcacgcac atccgtgtct gccgcccgat
     8821 gatccccagc acctgcaccc aggttctcgg cttgtgagtc gcctgaaaat gtccggactc
     8881 ccacaccctc accggcaatc cccgcgcaag aaaagcctgc cgcacactcg ccgcattcct
     8941 caccccgcac gcgcccgcca ccgttttgca cggcacgccc ttcatgtgca gcctgtgcag
     9001 ccccgccaca aagcgctcac tccaccaccg aggatgattt ttcggcgccg ccatcacgcc
     9061 gccctccttt ccatcagagc tgcccgcgcg tgtgcttcgg ccagtttcgg aggcacggca
     9121 ttgccgatct gcttgtgaat catctcctcc gtgcgtccgc ggaactcgta gctgtccggg
     9181 aagctctgcg cccgcgcgtt ctcccgccat cgcagcatcc ggtagccgat gtccagcagt
     9241 ccttccccgc cgccctccag cggcaccgca agcccaaagc gcggcttgca tgtcaccgtg
     9301 ttcagcggct cgtcacaact cgcgtgcgtc cccgtcccgt aatactccac cagcatcggc
     9361 tgcatcagcc cgatggcccc ggcggtggag acagtgggca ccggctctgt cacaggccgc
     9421 gcgcgtccat cgctctgctg ggggatcagg catggcgtga tgagcgcgtg atggctcccg
     9481 cagcacgcca ccgttgacaa aggctcgccc acgggccgcg ccgtgtagcc cgcattcgct
     9541 ccagtcccgc gcagggtcac gatgaaaggt tgcgcaagcc cccagctctg cccgcccacc
     9601 acggtcccca ttggctcgtc aatgccccgc acacgattcg ggcgcgcgtc gctgccgaag
     9661 ttgctctgca ccaaaaacgc ctcgatgccc tgcgctctca ggccggcctc gatgcggcgg
     9721 agagtgttgt tcgacaacgg ctttttgcgc ccaaagatcg acgggcaagg aatgctccag
     9781 tcaatcacat gatcacgcgc gctcagccaa gctggcagct cggtgaacag atcgccggtc
     9841 gcatctggct tgcggtgggt ggcgtcgggc cacaccagcc gcgtgccacg gcgggcggcc
     9901 tgcacaatga gccgctggcg cgtcgtcggg gctccgaagt tggcggcatt ctggatgcgc
     9961 cattcgacgc ggtagccaag ggtgcgcaga caggtgaccc actcctgaaa cagtgcgccg
    10021 cgatgcttca cgatcgggcg gcccttcacg tccagcggtc cccagtccag gaactcctgg
    10081 acattctcca cccagattcg cgctggataa atgcgctctg cccagtccag cacgcgccgc
    10141 gctccgcacc ggcgctgatc gtccaccggc aaaccgccgc gtgccttgct gaaatgggtg
    10201 cattctggcg aagcaaagag ccagtccagc tccccgcgtt tgaagaacgt ctcaggccgc
    10261 acatcgttga catccgcgca cagatgacgc gcacccggat ggttcgcgct gtgcgtctca
    10321 atcgccacgt cccaatgatt gatcgcggtc agctccagcc gcacgccgca ggcatccgcc
    10381 gccatctgcg ccccggtcga agtcccgccc gctccgcaga acagatccgc tgctttgatg
    10441 ttccgcgcct tcatcgtcca cccccttcca gattcggaaa ttcattccac tcgcggcctt
    10501 ccagcaaacg gcccgctctt tttttcccga cgcgccgcct gggagaatct tcaaactcgc
    10561 cggactcgcc aagaaatacc ggaccatctg cgagattgcc agacgaccat tcaccccact
    10621 gcttgaagaa gaacggcaca gaggcggctt cgcactggtc ccgcagactg cgcgcccaat
    10681 cggggtgcat cggtctcgct cccggcccgg actcgccgcc gacgatgacc cagtgaatgc
    10741 cgttgagtgg cggagggcca tcaaaatact cgccgacacg ccacgtcgtc accttgatgt
    10801 gaggactgtg cagatccacc ggacccagca gcggctcgca gctcagaaac cgcaccttgg
    10861 ccgggatgct cagcaagcgc ggaatgcgct catccgcccg cgcttggtct tccacagagg
    10921 ttccgatcca gacgttggcg gggatgtagt tgcccgtagt ccaatccgtg atccattcat
    10981 aaaatgcccc ggcgtctttc cagtcaaagt caggcgtggt gcgtggttcg ccacaataag
    11041 actgccttgc cattttctcg acctcaagaa tgcgtccagc ccaattctgc ggccttttcg
    11101 tcagcaaaag ccagtcgaga tgcggcgtgc taaaaatcaa ctctagcaga cgggcaagcc
    11161 attcaatcgg cacctcgtca tccagccagt ccgcgaggct ggcgcagaaa acgcgcgggc
    11221 ggggacaggg tagcgcctcc cattcagaga gcgggagctc gcgcgtcagt tcacggcggc
    11281 tgcttgtggc gagcttaatc agcaccgtcc gctctacgat gtcgcgattg tcccatgtcg
    11341 tcacattcgc ctccgcgcgc caaattgcgc cgttgagcac cctcgaattt tgcgccgcca
    11401 cagcccactt caacggctgc ctccacatgt cattactcgc cagcacgcgc ggcttccctt
    11461 tgccccattc acccagcacc gccggattcc gccggctcag cgtctccgcg taacagtgcg
    11521 cacagccggg agataccttc gtgcaacccc gccacgggtt gaaggtgtgg tcgcaccatt
    11581 caattttgga gttctcagcc atcttcccgg ccctcctttt ttgcgtgaac cgtgaagcgg
    11641 tcaatcgccc ggaatccggc cgcggccttg tgcccgccgc ccttgaactc cttcgccacc
    11701 acagacacgt cgaagtcgtc atgccgcgct cgcagcgagt aggcaaacgt cccgtcatcc
    11761 agcgtcatgt agatcgccgc gaacggcgcc gtgggatgca gctccagcag ccggtgaccg
    11821 atctcgcttt gcagcaccgg actggcgacg gccggcaccg tccagttcag cacgcgcatc
    11881 cagtggggat gcttgcagag ctgatcaacg gtcagtgcat cgtaggtggc gaggatttct
    11941 cccgcctgaa tgaggcgttc cttcgccgtc gtcacacccg agtgccatag cgcgtggatc
    12001 gtcttccaag ccccgaactc gcgcggcgtt cccttccaca gcccagcgtt gatctcgcgc
    12061 gatgccggca gcttccattg ccacaagtca cgatcctgga tgtacagcag catctccggc
    12121 acgtcggtgc cgtggaagtg ctcccaggcg agcaccgcgc cgcttttttg caggtcaaac
    12181 gtggcgaaat ccagccctcg cagcgcctcc tgtgccgtgg ggtgatggtc cagcaccgtc
    12241 acctggcagc gctcagccag cgcctccagg atggggcgcg gataactgaa gtccaccatg
    12301 atcaccatcg ccccgtcagg cagatcaggc agcggttccc cgtaattcac aggcatgtaa
    12361 gtggcggcat tgccaatcgt catccaagcc gcccaggcag ccgcaaagcc gtcagggcag
    12421 ttggcgtggt aaagcacaaa aatcggtgtc gtcttcatgg tcgtgttgtt ggtggatgcc
    12481 cgcgccggcc tccatcgcgc gccactccca gggcaacccg gcagagtgcg cggcgcgggc
    12541 aaagggttca gagctgcaat tcctccagca ggaattgcag cctctggttc aaggtcatca
    12601 gccgttcgtt cgtgttgttg atcaaattac ccagcaccgt cgagctcagc ggcgtttcgg
    12661 acttcggttt gggcgcctca ggcacagggc agcacacgtc ggccaagcgc cctcgtagcg
    12721 tttcatagag tgcctctaac tcagtgagct gttcgcgctg gaagcacagc agcccttcaa
    12781 tttcgcggac atcgcgcgac tcagcggggt ctttaagttg cgccgacagc gcgccgttgc
    12841 cgctttggaa tgtgtctgtt ctcatggtgt gtgtgttttt ggtttggcct cctccccagg
    12901 cagcacccgc cgcctggaga gagggcggat cgttcagccc tcagcggcgg gcgtggattc
    12961 gggcgtcggc gtgtcggcgg caggctcctg gccgccgctt tccggctcgg gcggagtctc
    13021 agaagtcggt gaagctgccg gaatcattcc cagggtccac ggactcggcg ggcggcggcc
    13081 cctctttttt ttcagtggcc tttcgcgcgg gctgcttcgt cggcttcggc gcggtgcgct
    13141 ccagcagcgt catcaccccc tcatgcacct cccgcagcag cgtattgaca tgcagcgccg
    13201 ggttcgccgt atctggcact gactggcttt ccttttcaag cggcacaggg atagtcctgt
    13261 cgtgaatttt cagcgccact tgtttgccgc caacaagcaa gctggcggtc tcaatcgagt
    13321 ggccattgct atcgagttgt tgcaagagct tgcggagccg ctgctcaatc gagttgggtt
    13381 cgtccccact gtgaccagag ccagtgagtg ttatgctgta acgtgtgttc atcggtggtg
    13441 tgttgtgttt ggtttatccg tgttgtcacg ggaagggtct gccgcgccgg atggccggcg
    13501 cggcattgtc aaaatttttg ccgcgtctga atgaatgagg ggggggtgag catctcgcgc
    13561 ggcgagatac ccccccccca tatttcgatg atcaggaggc gtttcggcat gtctagctat
    13621 gctgttcatc attgattttc agtatgttat tgaccgttag aaacagattc taaatctgtt
    13681 tgcccttctt ttgtgttcgc tcgatcaggt gtttcagcgg gcgtgacatc ccgcgattgc
    13741 ggcaaaaact ccgcgtcaaa taccattcca ttaggaggtg agggcagcgc cctggcttgg
    13801 gccaggaacc tcgcaaactc ctcttcgtct gccgatattt ttacttccac ccgctgagtc
    13861 actccacctg acaagagttg gcggatgtcg aagaggagct tcagatcgaa tatgctcggc
    13921 agattgcctt cgcgagcctg ggccagcacc tcatccaccg tgagagctag aatttcatcc
    13981 agcttctgga ctaccccgtt ggattccgat actacagcca ctttccactt tggatcgtgc
    14041 cgcaccctgt tcaccgtgca tggtgacact ttcagcgccc tcgtgatcgt cttcaccgag
    14101 accccacgcg ccagaaacca aacgattgcg cccaggatgt ctggccgccg ctgctccagg
    14161 cgagtcagat caggccagcg tccccgcgcc tcttccgtct cagcgactga gacggcgaat
    14221 ccaagctccg tggtgatctc ctcgctcatc gttcagcgtc tccagttgca gttgcagttg
    14281 cccgctgaga aaaaaaagag gcgcgccggc ggcgaagctt gagcctttca ggcaccatct
    14341 ctgcatcagc gcgcgtcact ctcaccaggt gcccaaactt cgtcactggc agtacaggct
    14401 tgccatcgcg gcacatttgc cgtgtccacc catacaccgt gggttccggg aaaccgtgca
    14461 gcgcggccac ctcgcgtatc gtcagctttt ggacagggcc acacggcctg ccgcgcttgc
    14521 gtttgggaga tggagcggga gagtccatgg aaaaaagaaa ggcgcggcgt caggcggcag
    14581 ttggccaaat cagcccctcg cgggcaaaca ccgtgtcagc ggcatggcag cacatggccc
    14641 gatagtgctc ccactcttgc ggtgttaatg tgcatgcctt gccgtcgcag gggaccacat
    14701 acagatcaga tcccggatga ctcccagcta tcacggcaaa gcgctccaca gcagcccctg
    14761 ccaggaaaaa aaggagctcc ggcgaaacgc ggggaagctc tcgtgttggc gccggcacac
    14821 tcatgcttcc agcacctcct caagtttggg cttcgcgtga tcaaagattg tcacctccag
    14881 cgtctcgcaa ttcagccagc cacatgcacc catgtcgcca ccagccagat gcagctcgac
    14941 gaccagggaa aacttgaccg ataccttgcc cgtcctgccc tgctcggcag cccgcagaca
    15001 caccaacatc tgcctggcgc gcgcgagggc tggctcacag ccggaaaaac aagccaccgt
    15061 cgagactgag ggtttcatca agtcatcgtt ccagtccacg tgctcaaaga tcacgaaatg
    15121 catccactca ggcaccttca cgcccgccgg caccttagcg tgaatgtgcc cctcgcgcag
    15181 tgcccagtaa ccacagcggc ccttggagcc ttccgtcagt agtagcgagg cagcgttcac
    15241 gatggcttcg gagagcggct tgctgttcac gccgccacgc cagcgtcacc gccagcgccc
    15301 tcccgttgga gttgccgcac acgtcgcagc gcccgcgccg cagtctctct cgcgcgagca
    15361 cggccctcag cggtcacata caaggccgcg cgcgccttcg cgcggctcgc tgcgtcaagc
    15421 cacaaggcga gcagggcggc atgcacctcc gccggtgttt tgaatgacac cctcacgccg
    15481 ccatcgccgc gtcaccgcgc gcgccctctg gttcggggtt gacgaggccc gcagtcaccc
    15541 acagcgcgcg cgtgtccctc agcagcttct ccagctcaga gagcgcgatg cgctttgccg
    15601 gatcgggagc cttgcgggca aacgtccgca tttccttcaa tgtctcgtca atggcctcag
    15661 tccgcgccgt gcgcagttcc tcgggccgaa agatttcagc gatggtgttc atggcgctga
    15721 attgatacaa tacgcaacat gcaacgcaac gaatatttgc gaaaatatgt aatttgatgc
    15781 agagtgttgc atggatcaaa ctccgcttgc cgccaaagtg ctctcagctt gcctcgaatc
    15841 tcaccaatgg gatcagtcag ccattgctaa acaatccggc gtgaaccgcg ccgtcgtctc
    15901 gtttcacttg accggcgcgc gccccatccg ggacgaccat ctttcagctt actgccgctg
    15961 tctggacgcc gaggaaaaag cgcgcctcgt ctccgcctgg ttgcgggacg cgctcgcccc
    16021 tgaggatgcc gccaccgtgc tcgatccagg cacgcaccgc ctccaggagg aggtgcgcgc
    16081 ctggcggccc ggcctcacag accgtcaaaa atcccagctc gaattttggg ccagccgcct
    16141 cgtcgcagat cccgatctcg ccgaaatctt cgacatcatc acccgccgcg caggctggca
    16201 aggctaaaaa aactcatcga gtaaagctcg attcagcgcc cttcagccgc ccacacactt
    16261 cgtcagcagc cacaacggca gcgtccatgc tgtgatatgc acgatgaaac ccagagcatt
    16321 gtagcagaaa tcccagccca catcgaagag agaccgtttc ggttcctgcc ggcctgggta
    16381 gattgtcggc ggcatggcca aaatggtgaa gagcggcgca aaaagcgccg ctaatacaag
    16441 ccagccccac catggcacct ccgcaatcat cgcacttgcc tcacgcgcac acgttcgccg
    16501 ctgccatcga gctgcatgaa agtcccctcg gaggtttgca gaatcatcac cttgtttacc
    16561 aggccaccgc caagggaact tttcatctcc tgctgctccc agagggtgcc atttgtcagg
    16621 cggaagacac gcccgttttg gaagccctga aacggcccgt ccagaaacga ttccatcaca
    16681 ccgcccgccg ccatcgggat cccagccagc gccgccgcag cgtgcagatt cccgcgccgc
    16741 gccgccgtct ccagccggcg ttccgggtgg tccttcatgg agcggccact tggcaccgga
    16801 gtcgccccgc ccagcccgcg cggagcgtgc agttcctgcg cagcatcatg ccgcacaagc
    16861 cgccgtcgga gatctacac
//
LOCUS       DAOMYJ010000105        16804 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Prosthecobacter sp. isolate AMR_MDS_5629
            NODE_1186_length_16804_cov_21.026330, whole genome shotgun
            sequence.
ACCESSION   DAOMYJ010000105 DAOMYJ010000000
VERSION     DAOMYJ010000105.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867850
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prosthecobacter sp. (wastewater metagenome)
  ORGANISM  Prosthecobacter sp.
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Verrucomicrobiaceae; Prosthecobacter.
REFERENCE   1  (bases 1 to 16804)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16804)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.30
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:34:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,742
            CDSs (total)                      :: 4,684
            Genes (coding)                    :: 4,676
            CDSs (with protein)               :: 4,676
            Genes (RNA)                       :: 58
            tRNAs                             :: 55
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16804
                     /organism="Prosthecobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1186_length_16804_cov_21.026330"
                     /isolate="AMR_MDS_5629"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1965333"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(269..2098)
                     /locus_tag="PLP58_12115"
     CDS             complement(269..2098)
                     /locus_tag="PLP58_12115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ09311.1"
                     /translation="MPGLHERMTLFITGGLAPKAMERQLRREAGPLVVLEHDTLLERG
                     RYPQVAEWLTLSDLAGFRDIEALRAQVALMFSEYLRQRPELGDQPGDSELRKAFLRGA
                     FLEGCFTRVYHMRLRELLWARHRFDRLVVAPGSGVNFAFWRAVAAEEGMEIIVLEPEW
                     KGRGLGRKIERWLCKRAQKKARSSIQPSLQPSILEGVPDDTGLPLAVCASRRVAPLLA
                     QEKEPCGFRVIQAGMKDLDPPDPAFEAAERERFSTWWRRWKTEVLPVVCAPDAKPCLA
                     PLRDLFERMGEEETAGTYPRVSALRARARAWLESRAPAAVIADMQMHEDEAVWSLAAA
                     QAGIPVIAYSYDCLALPDITHSPDILLMDGVRGRSRTLRAGYPAERMIDVRCHRIPAA
                     PPRTAAEIDAAFTARRPLVLSADTMAVINDPQEGLRGYRLLVEAARRLPEMDFVVKFH
                     PLRTPKSEERSFLGMDENEIQVKTRFIRALRPPRNFRLAPPEASMQDWLEKASVLVNT
                     ISLTGQEAFHFGIPVVFLCRLDADFITFPDMEKWMTPLLAENADQLVDALRRLATDAD
                     FRHAQARQQHHYQDQCFWPAGTSLAAGIRQAARLAAELGPFPR"
     gene            2482..2811
                     /locus_tag="PLP58_12120"
     CDS             2482..2811
                     /locus_tag="PLP58_12120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ09312.1"
                     /translation="MSLPLRKSLFVSPVNWRAPRALLLAAFLIEHAAIDAGEDDERVF
                     TQLEFIERAHHLAQRGEVEEERPSRMPAHPVHRFLRARGGVPLLVGERMRGLFTRNRR
                     RSTSGDF"
     gene            complement(2828..5509)
                     /locus_tag="PLP58_12125"
     CDS             complement(2828..5509)
                     /locus_tag="PLP58_12125"
                     /inference="COORDINATES: protein motif:HMM:NF028199.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heparinase II/III family protein"
                     /protein_id="HRJ09313.1"
                     /translation="MQIDPVEAPGGDLQVVAGLVVGLKSDEQVQESGAGALRADLREG
                     SERAAFTFWHRKTYLTMLEPDPKPPGWMDNRDYQHPNVLPFREGTGHRIKLVVWPEGE
                     GSRVRLFVDFMDRTVEEHLLPERIRAGVVKLFTMRGGNEPEVRQTSRFSEVRFAPVSS
                     EEARQLPSMAESVLRALDFNHPAIQPVSKAIQQGRMDEAKALLLRHMRTRREPKGPSL
                     DEVASMVLHPNWQKISDEAVAGRYATIGYFDGFVDAWTDTNGESHRWVLQKEPLQLNW
                     ARDNGHLNRHFHWVSMARAWQEKSDPKYPRQFSAEVLDWVSREPFFWERCPTVGKLNL
                     MDGTTFRWGYMNTSNIGRRLEMSWWPAYEVFRKSPDFSDEAHIAMLLGMLRQARLIMN
                     PSSFAAHDDGGAHTTLALLQTALLLPEFAESAEWKATAMKRWDVMLGAQFHPDGSHAS
                     LSTGYNWASITAIENYIRLFQSLGETPPEKYLAIHEKAVEYTMLLTTPTQGNIDLNDG
                     GWGLFDDRFRTATEWFPKRRDFEWMATKGAKGSPPDKTSVYFPNAGHYVMRTGWGPEE
                     KYLFFGVGPWGASHGKFDALNIYAQFGPHLLIRNAGRGSYSGIGNTKHAGRSLSFNTL
                     SPDWAQENSIPQWKQDMAIAFNPPKRRWVDNEHFSYAEGTFDYGWHNATEHIRGKWLR
                     QVIFVKGGDARKEGYHIVFDTVEPADSKPRTWRHPWQLGLNAGNIAIRESDRSTTAIT
                     PGAALQILPVGEMTPRMIQGQEKPELLGWRIYDTTANPWPVPTYEWQAEGTFSRAWVI
                     QMQADESEWPVESVEAHPAASPGELRFTIHRRDGGIDHVIRRLLGQPSIEFQGEKISG
                     DVAVIRTDAKGSKRDRLEVKDGEDSVARTSPQHPSKH"
     gene            complement(5928..7457)
                     /locus_tag="PLP58_12130"
     CDS             complement(5928..7457)
                     /locus_tag="PLP58_12130"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HRJ09314.1"
                     /translation="MKPLLHFLCALCVLDGKLLAAESPKPNFIVIFTDDQGWADLGSQ
                     GVREDLKTPHLDALAASGVRCTAGYITAPQCSPSRAGLLTGRYQQRFGLDAISDTPMP
                     VSELTIAERLKLAGYVSGMVGKWHLDASPSSGDWIKRNPKSVVRTPGQPLRIREPARL
                     AFSPAGQGFDEFYMNNRERRWANFRADGTSLKPEGEWLTETTGDRLDDETAGALAFLE
                     RNHAKPFFLYLAYSGPHTPLEATPERLARFPGEMPERRRTALAMLATIDDGVGRIMKT
                     LRKHGVEENTLIVFTSDNGAPLKKTMPDSPLTTEPNGKQSDIGGWDGSMNAPQFGEKG
                     MLTEGGIRVPFLWSWKGVIPAGQVFDHPVSSLDIAATVLKVAGGSLRGSAAAPEDAAK
                     AAATLDGVNLLPHLKGEVATPPHDALYWRFWKQAAVRAGDWKLIHLGDGTDLLFDLSQ
                     TDSETKNLAAQHPEKAAELRAKLETWTQQLRPPGIPKNGIQRERHWYEHYFQHPAPSK
                     P"
     gene            complement(7454..8509)
                     /locus_tag="PLP58_12135"
     CDS             complement(7454..8509)
                     /locus_tag="PLP58_12135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ09315.1"
                     /translation="MKFFFTVWLLLGTVLLHAAETPCQLLADTAFAEGFGAAFIYGKR
                     GEDGVIRGYRDIAPWLIHLIPDGPVKTLPVFKTHPWDFQEGLHHNYTNQRGEFVRELH
                     AHRLVVNHVLEVNTSEKLQFAQFNNDGLAKDDPQRDTKLVKRITSDRYGTLRIHYNSQ
                     NEIRNAAIAHTAQWARDTWPHFLLNQRFETPLPLADYDKLDFKVSFEVDAMQRLSPWP
                     GANRSSMNLNFMFHLKHKTDPEQKLFVGMMLFTSSEAKYRTHLGVEQHGQVFYRESVT
                     RDQPKPEFGQKRTITRELREMIRDALKQAQEKQPALSTNPDDFTLSNFSIGLEGMGHW
                     TTECEFSGLSLIGTPRP"
     gene            complement(8516..12244)
                     /locus_tag="PLP58_12140"
     CDS             complement(8516..12244)
                     /locus_tag="PLP58_12140"
                     /inference="COORDINATES: protein motif:HMM:TIGR02604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PmoA family protein"
                     /protein_id="HRJ09316.1"
                     /translation="MKPTFFILCLAAAVSLQARTSFDAKDADLNALPTAPKGFEVQLW
                     AKEPLVSNPCAMAFDAKGRLFVGMGPQWRAPRPDSPKDMVVVLEDRDGDGVAESKKVF
                     AEGFNSVQSIAWRGRELWVANSPDLTVVRDTDGDDVADEYVKVFTDLGNIEHCLHGLN
                     WGPDGCLYLSKGNSKGISLDGDAPKEPGRVAPKAFRELWGYPGPKGAPDLPPPSEVFT
                     RETYRATYQDPADDWGQTGGILRYDPATPSLTIHSRGYRNPWDIAFDSAFNWLGTDND
                     QTGGDRVFMPFQHAHFGWGHPWSPAWPGEGHLPTAPNSGQIIEGSYTGIVFADTPHFP
                     ESHRGVWFIGDWMTKKIYLYRPEWNGALNVPQGGRYEDFVVGGKSLFRPTDIAMGPDG
                     VLWVLGWGRDYGGTFDEQGIQNNEGRVYRIVAKDRPLVQSKRPAKPPAEWSFDELLAD
                     LGSWIPAWQIDARDELVRRGEVSVGPLLGVLEKPASQAQETWAVWTLAKINVNEVPPK
                     NDNVVLQMIRAGCTEPHDYITDPNPRYRLAAIEAMAAHGQPNGRILNRLISETDPVVY
                     HAGWRTIMAHATEPAMRALATDRNAGIRRAGVLMLMEKLLITEAEVLRLLQDSDESIR
                     QLAALWLSKVKGIEPGAAKDSGIPDAFPLAQNLRAESKHRYLSGTVRQGEPHYTDRAY
                     AIDKFPAFLAGTSMIRTPNADDGSGGDTLLSFDAPLDVTVYVAHDERVKAKPAWLTGF
                     GDSDSVITSTDKHSIFRLFAKDFPAGRITLGGNTADGKPGGKSHYFVILVPKPPDPSG
                     KVATLDEALAALATADPNRGEALFLANGGAGCAACHTMNGRGHAFGPDLTGAGDRFDA
                     RHILDSMLNPNAIITEGFSMMSVTMKTGGPQTGVLREQSGLHLTLAQPGGGLVKLERK
                     RIAKEEMHPVSMMPPFGAILNAQQLAELAAFLLSQKAAPKTGFHLQQHDDHFEVVLDG
                     QRIATYQFRHDKVLRPVWINLVTPGRRQVTRNYPPRVPDDVDPGYKAESGGIIHPHIH
                     TGVWLGFGDIDGHDYWRNTARIEQLELIGVKSSADRLSFEVLNRFLTTDGQREVCRQR
                     VRYELARHPQGWKLDLAAEFFNDERDFYFGDQEESGLGVRVASPLRVQGGSGRITNSL
                     GEVNYAGTWGHEAAWWDYSGTLDGKPCGIFVQPHATNPRPCWGHTRDYGVMVLNPFPR
                     QPKESREPYVKTVVKKGESFRLGYTVIVHEGAFQPARP"
     gene            complement(12241..13710)
                     /locus_tag="PLP58_12145"
     CDS             complement(12241..13710)
                     /locus_tag="PLP58_12145"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase"
                     /protein_id="HRJ09317.1"
                     /translation="MMRLVIFLLTLCSPLFAAAQPNIVLIFADDLGYGDLGCFGSTKI
                     KTPHLDKMAAEGLRLTSFYAQAVCGPSRAALMTGCYPIRIGEPGNKKNQHTVMHPKEV
                     TMAEVLREAGYATACIGKWHLGEHAKDSPTGFDPATMPNGQGFDYFYGTPLFNGATVN
                     VADSKFRSTILRNEQVVTPAVESWDHITQDYTREALDFIRQQREKPFFLYLAHNMPHI
                     PLGASEKFKGKSAGGLYGDAVEEMDWSCGEIINTLRELGLAENTLVIFTSDNGPWVET
                     KRGMEPDGKAFIPRDHSGNADPLRGYKMLTWEGGLRVPCVAWWPGKIPAGTSSDAITS
                     TIDLLPTFASIAGGKTPSDRVIDGRDLTPLLRDPATTASPHEAFFYYCFTHLQAVRSG
                     KWKFVRKRPAHPPWVGWSGRFAGNAVNEFSLYDLDNDIGETRNVAAEHPEVLDRLRQF
                     AHEARMDLGDYDHTGSGARFFDGPAPARKAPGKGKGKAK"
     gene            complement(13787..15379)
                     /locus_tag="PLP58_12150"
     CDS             complement(13787..15379)
                     /locus_tag="PLP58_12150"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HRJ09318.1"
                     /translation="MKPCLFLLALCPALLAAQPNVVFIYCDDLGFGDLACHGHPRIQT
                     PNLDRMAKEGTDFWSFTVVNPVCSPSRTGIVTGQFPSRWGVHQHFASHEQNVARNMPD
                     WLDPKAPLLPRVMKEAGYKTAHFGKWHLSGGNIEGAPQPSAYGYDDAAVWTGPGAHVF
                     DGMKYEFAKGEGSAHDKQAASWISVAATDHALRFIREAKGAPFYLNLWLHETHHLVSA
                     TEDDKKPYPDVPEPQRTYYAAVSRADRQVGRVLDLLDELNIANNTLVMFSSDNGPENS
                     HAQPGQKFYHSQGSTGGLRGRKRSLLMGGVNVAFLARWPGVVPAGRVDKETALAGVDV
                     FPTVLAATGAKAPAGYVSDGEDMLAAFKGGTQPRTKPVFWWWQGGHSGDDWPAFAMRD
                     GPHVLILDETKQRAELYDVIADRFQSNNLAAADPERVKRMTAAIDEWFATLPKNVDPA
                     LRAKAAPKPAAKPSKPAEITPETRLRAFKNWDKNQDGHLTLEEYRNGLAKKDNVENRF
                     KNFDKNSDGKVTREEFAGKAAP"
     gene            complement(15376..>16804)
                     /locus_tag="PLP58_12155"
     CDS             complement(15376..>16804)
                     /locus_tag="PLP58_12155"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HRJ09319.1"
                     /translation="PNIILMMGDDHGWEETGYNGHPHVKTPVLDEMAATGLKFERFYA
                     AHPSCSPTRASFLTGRHPNRMGTFAPGWSFRPEEITSAHLLGKAGYHCAHLGKWHVGA
                     VKKESPVSPLAMGFHECLSHDNFFEMNPSLSRNGGPPEVFKGESSEILIREAIASIGR
                     AQKEGKPFLQVIWFGSPHEPYSGLPEDLALYDDLPAKYTKKVKLTSNETGGPTTRPQG
                     EVLRERYAEITAMDRAIGTLRKHLAQNGLRENTLLFYCGDNGTSPDAALGFPHRGAKA
                     TFYEGGTLVPGLIEWPARIPEPRSTSVRASTSDLLPTVAAIAGQTLPDRPLDGMDLAA
                     VLDGKMTARPGALHFWEYRPAGGKKGEMKPYIAPELQQGTTPLVKKMGNKATRDFTNF
                     HHPVISEEDYLGARSIIEGDHKLVIHEQKGGAAKVELFDLKADPAEKTNLAEKQPDLV
                     KSLQSKLRAWQESVLKSLTGADYQP"
BASE COUNT         2826 a         5015 c         5609 g         3354 t
ORIGIN      
        1 aaaagtgtaa cccatgtgcc cggtcaatct gttacccatc tgcccggtcc gaacctcctc
       61 ccggcccgaa cccccgtttc cctgtctgtc tggatggggt cggcttcatc gggaggccag
      121 agcagacagg agtgtctgca tcacggctgg agcactcttg gcggccgcgt ccaacattgg
      181 agtgaagtta gctggcacgg gagaggactc ctctgcggac gttcatcgaa caagctgaag
      241 cctggaccaa acgagacacc gtggcacgtc agcgcgggaa ggggccaagc tcggcggcga
      301 ggcgcgcggc ctgacggatg ccggcggcga ggctggtccc cgcgggccag aagcactggt
      361 cctggtaatg atgctgctgg cgtgcctggg cgtggcggaa gtccgcgtcc gtggcgaggc
      421 ggcgcagcgc gtccacgagc tggtcggcgt tttccgccag caggggtgtc atccatttct
      481 ccatgtcggg aaaggtgatg aagtcggcgt cgaggcggca gagaaagacg acggggatgc
      541 cgaaatgaaa cgcctcctgg ccggtgaggg agatggtgtt gacgagcacg gaggcctttt
      601 ccagccagtc ctgcatggag gcctccggcg gcgcgaggcg gaagtttcgc ggcgggcgca
      661 gggcgcggat gaagcgtgtc ttcacctgga tctcgttctc atccatgccc aggaagctgc
      721 gctcctcgga cttcggcgtg cgcagcgggt ggaacttcac gacgaaatcc atctccggca
      781 gccggcgcgc ggcctcaacc agcaggcggt agccgcgcag cccttcctgc gggtcgttga
      841 tgacggccat ggtgtcggcg ctgagcacca gcgggcggcg agcggtgaag gcagcgtcaa
      901 tctcagccgc tgtgcgcggc ggcgccgcgg ggatgcggtg gcagcgcacg tcaatcatgc
      961 gctcggcggg gtaaccggcg cgcagcgtgc ggctgcgccc gcgcacgccg tccatgagca
     1021 gaatgtccgg gctgtgggtg atgtccggca gcgccaggca gtcgtagctg taggcgatga
     1081 ccggaatgcc ggcctgcgcg gcggcgaggc tccagacggc ctcgtcctcg tgcatctgca
     1141 tgtcggcgat gacggcggcg ggcgcgcggc tctccagcca ggcgcgggcg cgggcgcgca
     1201 gcgcggagac gcggggataa gttcctgccg tttcctcctc ccccatgcgc tcaaagagat
     1261 cgcgcaatgg agccaggcag ggcttggcgt ccggcgcgca gacgacgggc aggacctccg
     1321 tcttccatct ccgccaccag gtggaaaaac gctcgcgctc ggcggcttcg aaggcggggt
     1381 ccggcgggtc gaggtccttc attccggcct ggatgacacg gaagccgcaa ggttccttct
     1441 cctgcgcgag aagcggcgcg acgcggcggc tggcgcacac ggccagaggc agcccggtgt
     1501 cgtccggcac gccttcgaga atggagggct gaaggctggg ctgaatggag gaccgggctt
     1561 tcttctgcgc gcgcttgcag agccaccgct cgatttttcg ccccagtccc ctgcccttcc
     1621 actcaggctc aaggacgatg atttccatcc cctcctccgc ggccacggca cgccagaagg
     1681 cgaaattcac cccgctgccg ggcgcgacca cgaggcggtc aaaacggtgc cgcgcccaca
     1741 gcagctcgcg cagccgcatg tgatagacac gggtgaagca gccctccagg aaagcgccgc
     1801 gcaggaaggc cttgcgcagt tccgagtcgc cgggctggtc gcccagctcg gggcgctggc
     1861 gcagatactc ggagaacatg agcgcgacct gcgcgcgcaa ggcttcgatg tcgcggaagc
     1921 cggcgaggtc ggagagcgtg agccattctg cgacctgcgg gtagcgtccg cgctccagca
     1981 gggtgtcatg ctccagcacg acgagaggcc ccgcctcgcg ccgaagctgc cgctccatgg
     2041 ccttcggagc caggccgcca gtgatgaaca acgtcatgcg ctcatgaaga ccgggcatgg
     2101 cgcaaaagca aacatcaaca gcctccagga tcatgcgccc gaagccggcg gtggcgcgga
     2161 tgaagacttg ccaattgcct ccccgaaggc ggccaaccgc acccggctac aaacgaaacc
     2221 ccagccgcgc atggtcggca ggaggcgtca tgtccatttc ggttcaaatc agtccagttt
     2281 ggccggttct ggccgatgtg agtcgattcg gcggcgatgt ggcagccgtg acgactgcac
     2341 caaataggct ggcttcactg ccgaatagcg gcgaggcgat ccgccaggcg cctcgcgatt
     2401 tccggatgct cgcggatcac tttcttcgtc tagcgagggt catgggtgag gtggtagagc
     2461 tgctacccgg gtgcatcggg cttgagtttg ccgttgagga aatcgctgtt cgtctcgccg
     2521 gtgaattgga gggcaccgcg tgcgctgctg ctggccgcct tcttgattga gcatgccgcc
     2581 attgacgcgg gtgaggatga cgagcgtgtt ttcactcagc ttgagtttat cgagcgtgcg
     2641 catcatctcg cccagcgtgg cgaggtagag gaagaaaggc cttcccgcat gccggcgcat
     2701 ccagttcacc gctttcttcg cgcgcgtggt ggcgtgccgc tcctcgtcgg agagcgcatg
     2761 cgcggccttt tcaccagaaa tagacgacgc tcaacgtctg gcgacttttg agcaatgccg
     2821 ggctgattca atgtttgctt gggtgctgcg gtgatgttcg agcgaccgag tcttcgccat
     2881 ccttcacctc caatcgatct cgtttggagc ctttggcgtc cgtgcgaatc accgcgacat
     2941 cgccagagat tttttcgccc tgaaattcga tggacggctg gcccaggagg cggcggatca
     3001 catggtcgat gccgccgtcg cggcggtgga tggtgaaacg cagttcaccc ggagacgcgg
     3061 cgggatgtgc ctctacggac tccaccggcc attcggattc atcggcctgc atttggatga
     3121 cccacgctcg gctgaaggtt ccctcggcct gccattcgta ggtcggcacg ggccagggat
     3181 tggcggtggt gtcgtagatg cgccagccga gcagttcggg tttctcctgg ccttggatca
     3241 tgcgcggggt catctcgccg acagggagga tctgcaaggc cgcgccggga gtgatggcag
     3301 tggtgctgcg gtcggactcg cggatggcga tgttgcctgc attcaggccg agctgccacg
     3361 ggtgccgcca ggtgcgcggc ttgctgtcgg cgggttcgac ggtgtcgaaa acgatgtggt
     3421 agccttcttt gcgggcatcg ccgcctttga caaagatcac ctgccgcagc cacttgccgc
     3481 ggatgtgctc ggtggcgttg tgccagccgt agtcgaaggt gccttcggcg tagctgaagt
     3541 gctcgttgtc cacccagcgg cgcttgggtg ggttgaaagc gatggccatg tcctgcttcc
     3601 actgcgggat gctgttctcc tgcgcccaat ccggcgagag ggtgttgaag gaaagcgatc
     3661 tgccggcgtg cttcgtgttg ccgatgccgc tgtagctgcc gcgtccagca ttgcggatga
     3721 gcagatgcgg gccgaactgc gcgtagatgt tgagcgcgtc aaacttgccg tgactggcac
     3781 cccacggtcc gacaccgaag aagagatact tctcctccgg cccccagccg gtgcgcatca
     3841 cataatgacc ggcattgggg aaataaacgg acgttttgtc cggcggcgat cccttcgcgc
     3901 ccttcgtcgc catccattcg aagtcgcggc gcttcgggaa ccactcggtg gcggtgcgaa
     3961 aacggtcgtc gaacaagccc caaccgccgt cattgagatc aatgttgccc tgggtcggtg
     4021 tcgtcaggag catcgtgtat tcgaccgcct tctcgtgaat cgcgaggtat ttctccggcg
     4081 gagtttcgcc gaggctctgg aacaagcgga tgtagttttc gatcgcggtg atggaggccc
     4141 agttgtagcc cgtgctcagg ctggcatggc tgccgtcggg atgaaactgc gcgccgagca
     4201 tcacgtccca gcgtttcatc gcggtggcct tccactccgc gctctccgca aactccggca
     4261 gcagcagcgc ggtctgcagc agcgccagcg tggtgtgcgc gccgccgtca tcatgcgcgg
     4321 cgaagctgct cgggttcatg atcaaacgcg cctgccgcag catgcccagc agcatggcga
     4381 tgtgcgcctc gtcgctgaaa tcgggcgatt tccggaacac ctcgtaggcc ggccaccacg
     4441 acatttccaa acggcggccg atgttgctgg tgttcatgta gccccagcgg aaggtggttc
     4501 catccatgag gttgagcttt cctaccgttg ggcagcgctc ccagaaaaac ggctcgcgcg
     4561 acacccagtc gagcacctcg gcgctgaact ggcgtggata tttgggatcg cttttttcct
     4621 gccaggcacg ggccatcgac acccaatgga aatgccggtt gagatggccg ttgtcacgcg
     4681 cccagttgag ctgcagtggc tccttctgca aaacccagcg atgtgactcg ccattcgtgt
     4741 ccgtccaggc gtccacgaag ccgtcgaagt agccgatggt ggcgtaacgc cccgccaccg
     4801 cctcgtccga gattttttgc cagttcgggt gcaacaccat gctggccact tcatcgaggc
     4861 tcggcccctt cggttcacgg cgcgtgcgca tgtgtcgcaa gagaagtgct ttcgcttcgt
     4921 ccatccgccc ttgttggatg gccttgctga ccggttggat cgcagggtga ttgaaatcca
     4981 aggctcgcag cacgctctcc gccatggaag gaagctgccg cgcttcttcg gagcttactg
     5041 gggcaaaccg cacctccgag aaacggctcg tctgccggac ctccggttca ttgccaccgc
     5101 gcatcgtgaa aagcttcacc actccggcgc gaatccgctc cgggagcaga tgctcctcca
     5161 ccgtccggtc catgaaatcc acgaacagcc gcacgcgcga tccttcgccc tccggccaaa
     5221 ccaccagctt gatgcggtgt ccggtgcctt cgcgaaacgg cagcacattg ggatgctgat
     5281 agtcgcgatt gtccatccag ccgggcggct tcggatctgg ctccagcatc gtcagataag
     5341 tcttccgatg ccagaacgtg aacgccgccc gctcgctgcc ttcgcgcaga tccgcccgca
     5401 gcgcgccggc tccgctctcc tgcacctgct cgtcgctttt caatcccacc accaatcccg
     5461 ccaccacctg aagatctccg ccaggggcct ccactggatc gatttgcatc tccaccgcga
     5521 atgactccgc cgcaggatca atgccttcga cttccgattc gctccatgga gctgtccggg
     5581 ctgtgagtgt gactggttgg gactcctcct gagcggaggc agagagcgag aggcaagtga
     5641 tggcggtaaa gacagcggtg agtgtgcggg aagcgatttc aaggctgccg aaccccaacg
     5701 ccctttttct tccgcgcaat aaggcaggag accccgatga ctcacggtgt ctttcgcact
     5761 cgcctttcat atgaaacatc atctggattg ggttgcggcg tgacggcttg ggaatctttg
     5821 agaaaagctt cgatcttggt gtccatagcg gcgacgcggt cgggatgccg cgcggcgaag
     5881 accggagcag acagcagtat gatgaagaca agtgatgcag gaggctttca tggcttggac
     5941 ggggccggat gctgaaagta atgctcatac caatggcgct cgcgctggat gccgttcttc
     6001 gggatcccgg gcggccggag ctgctgggtc caggtttcga gtttggcgcg gagttcggca
     6061 gctttttcag gatgctgcgc agcgagattc ttggtctcgg aatcggtctg gctgaggtcg
     6121 aagagtaagt cggtgccgtc gccgaggtgg atcaacttcc aatcgcccgc acggacggcg
     6181 gcttgcttcc aaaagcgcca gtagagggcg tcgtggggcg gtgtggcgac ctcgcctttg
     6241 agatgcggga gcaggttgac gccgtccaaa gtggctgcgg ctttagccgc atcctctggg
     6301 gctgctgcgg agccgcgaag cgaaccccca gctactttca ggaccgtggc ggcgatgtcg
     6361 agcgacgaca ccggatgatc aaagacttgt cccgccggaa tgacgccttt ccacgaccag
     6421 aggaagggca cgcggatgcc gccctcggtg agcatgcctt tttcgccgaa ctgcggagcg
     6481 ttcatggatc catcccatcc gccgatgtct gactgtttgc cattgggctc ggtggtcagc
     6541 ggtgagtccg gcatggtctt cttcaagggc gcgccgttgt cgctggtgaa gacgatgagc
     6601 gtgttttctt cgaccccatg tttgcgcaag gtcttcatga tgcggcccac gccgtcatca
     6661 atcgtggcga gcatggccag cgcggtgcgg cggcgttcgg gcatctcgcc ggggaatcgc
     6721 gcgaggcgtt ccggcgtggc ctccagcggc gtgtggggtc cgctgtaggc gaggtagagg
     6781 aagaagggct tcgcgtggtt gcgttcgagg aaggcgagtg ccccggcagt ttcgtcatcg
     6841 agccggtcgc cggtggtttc ggtgagccac tcgccttcgg gcttcaagct ggtgccgtcc
     6901 gcgcggaagt tcgcccagcg gcgctcccgg ttgttcatgt agaactcgtc gaaaccctgt
     6961 cctgcgggtg aaaaggcaag tcgcgccggt tcccggatgc gaagtggctg gcccggcgtg
     7021 cggacgacgc ttttcggatt gcgtttgatc caatcgcctg acgacggact ggcatcgaga
     7081 tgccacttgc cgaccatgcc gctgacgtag cctgccagct tgaggcgttc cgcaatcgtt
     7141 agctcggaca caggcatggg cgtgtccgaa atcgcatcca ggccgaagcg ctgctggtag
     7201 cgccccgtga gcaaaccggc gcgagagggc gagcactgcg gcgcggtgat gtaaccggcg
     7261 gtgcaacgca ccccggaagc ggcgagggca tcgagatgcg gcgttttcaa atcttcgcga
     7321 acaccctggc tcccgaggtc ggcccagcct tgatcgtcgg tgaagatgac gatgaagttg
     7381 ggcttcggtg attcggcggc gaggagtttg ccatcgagga cacagagggc gcagaggaaa
     7441 tggaggaggg gtttcatggt cttggagttc cgatcaggct cagcccgctg aactcgcatt
     7501 cagtcgtcca atgccccatg ccttcgagac cgatgctgaa gtttgagagg gtgaaatcgt
     7561 cgggattggt cgaaagcgcg ggctgcttct cctgcgcctg cttcagcgca tcgcggatca
     7621 tctcgcgcag ctcgcgggtg atggtgcgct tctggccaaa ctcgggcttc ggttggtcgc
     7681 gggtgacgct ttcacgataa aacacctgtc catgctgttc gacgccgagg tgggtccgat
     7741 atttcgcctc gctgctggtg aagagcatca tgccgacgaa tagcttctgc tcgggatcgg
     7801 tcttgtgctt gaggtggaac atgaagttga ggttcatgct ggagcgattg gctccgggcc
     7861 agggtgagag ccgctgcatt gcatccacct cgaagctcac cttgaaatcg agcttgtcgt
     7921 aatcggcgag cggcagcggc gtctcgaagc gctggttgag gaggaagtgc ggccaggtgt
     7981 cgcgtgccca ctgcgcggtg tgggcgatgg cggcgttgcg gatctcattc tgcgagttgt
     8041 aatgaatgcg cagcgtgccg taacggtcgc tggtgatgcg tttgacgagc ttggtgtcgc
     8101 gttgcggatc gtctttggcg agtccgtcgt tgttgaactg ggcgaactgg agtttttccg
     8161 acgtgttgac ctcaaggacg tgattcacca cgaggcggtg agcgtgcagc tcacgcacga
     8221 actcgccgcg ttggttggtg tagttgtggt ggagtccttc ctggaagtcc caggggtgcg
     8281 ttttgaacac cggcagcgtt ttgacaggac cgtctggaat aagatgaatc agccacggag
     8341 caatgtcgcg atagccacgg atgacaccat cctcgccacg cttgccgtag atgaaggcgg
     8401 caccaaagcc ctcggcgaag gcggtgtcgg cgagcagttg gcaaggtgtt tccgctgcgt
     8461 gaagcaagac ggtgccaagg agcaaccaga cggtgaaaaa gaatttcatg ggggatcaag
     8521 gacgggccgg ttgaaacgct ccttcgtgca cgatgaccgt gtagccgagg cggaagctct
     8581 ctcccttttt caccacggtc ttcacgtagg gttcgcggct ctctttgggc tggcggggga
     8641 agggattgag aaccatcacg ccgtagtcgc gggtgtggcc ccagcagggg cgcgggttgg
     8701 tggcgtgggg ctgaacgaag atgccgcagg gctttccatc cagggtgccg ctgtagtccc
     8761 accacgcggc ttcgtggccc caggttccgg cgtagttgac ttcgccgagg ctgttggtga
     8821 tgcggccgct gccgccctgc acgcgcaggg gcgacgcgac gcgcacgccg agtccgcttt
     8881 cctcctgatc gccgaaatag aagtcgcgct cgtcgttgaa gaactccgcc gcgagatcaa
     8941 gcttccagcc ctgcggatgt cgcgcaagtt cgtagcgcac gcgctggcgg cagacttctc
     9001 tttggccgtc ggtggtgagg aagcggttga ggacttcgaa cgagagtcga tcggcagagg
     9061 acttcactcc aatcagttca agctgctcga tccgcgccgt gttgcgccag tagtcgtggc
     9121 cgtcgatgtc gccgaagccg agccacacgc cggtatggat gtgcgggtgg atgatgccac
     9181 cgctttccgc cttgtagccg ggatcaacgt cgtccggcac gcggggcggg tagttgcggg
     9241 tgacttgtcg ccgtccgggc gtgacgaggt tgatccacac cggacgcagc accttgtcgt
     9301 gacggaactg gtaggtggcg atgcgctggc catcgaggac gacctcgaaa tgatcgtcgt
     9361 gctgttgaag gtgaaagccc gttttcggcg cggctttctg actcaggaga aaggcagcca
     9421 gctcggcgag ctgctgcgca ttcaaaatcg cgccaaaggg cggcatcatc gagacgggat
     9481 gcatctcttc cttggcgatg cgtttgcgct ccagcttcac caaaccaccg ccgggttgcg
     9541 ccagcgtgag gtgcagtccg ctctgctcgc gcagcacgcc ggtctgcgga ccgccggttt
     9601 tcatggtcac gctcatcatt gagaagcctt cggtgatgat cgcgttgggg ttgagcatcg
     9661 agtcgaggat gtgccgcgcg tcgaagcggt cgcccgcgcc ggtcaggtcg ggtccgaaag
     9721 cgtggccgcg tccgttcatc gtgtggcagg ccgcgcatcc ggcaccgccg ttggcgagga
     9781 agagtgcttc gccgcgattt ggatcggcag tcgcgagagc ggcgagagct tcatccagcg
     9841 tcgcgacttt gccggaggga tcgggcggtt tgggcacgag gatgacgaag tagtggcttt
     9901 tgccgcccgg cttcccgtcc gccgtgtttc cgccgagcgt gatccggcct gcggggaagt
     9961 ctttggcaaa gaggcggaag atgctgtgtt tgtccgtgct cgtgatgacg ctgtcgctat
    10021 cgccgaatcc agtgagccac gcgggttttg ccttcacccg ttcgtcgtgc gccacataaa
    10081 cggtgacatc gagcggcgcg tcgaagctga gcaaagtgtc cccaccgcta ccgtcgtcgg
    10141 cgttcggtgt gcggatcatc gaggtgcctg cgaggaaggc gggaaatttg tcgatggcat
    10201 aggcgcggtc ggtgtagtgg ggctcgcctt ggcgcacggt gccgctcagg tagcggtgct
    10261 tggattctgc acggaggttt tgcgccaggg gaaaggcatc gggaatgccg ctgtccttcg
    10321 ccgcaccagg ctcgatgccc ttgaccttgc tgagccaaag cgcggcgagc tggcggatgg
    10381 attcgtccga gtcctgcagc agccgcaaca cctcggcctc ggtgatgagg agtttttcca
    10441 tcaacatgag cacgccagcg cggcggatgc cagcgttgcg atcggtggcg agtgcgcgca
    10501 tggcgggctc cgtagcgtgc gccataatgg ttcgccaacc agcatggtaa acaacgggat
    10561 cggtttcgct tatgagccga tttaagattc ttccattcgg ctgaccatgc gcggccattg
    10621 cctcgatcgc ggcaagcctg tatcgcggat ttggatcggt gatgtagtcg tgtggttcgg
    10681 tgcatcctgc ccgaatcatc tggagaacaa cgttgtcgtt tttgggcggg acctcattga
    10741 cattgatctt cgccagcgtc cacaccgccc acgtttcctg cgcctgactc gccggtttct
    10801 ccagaacccc cagcagaggc cccacactta cttcaccgcg tcggaccagc tcgtcccgcg
    10861 catcgatttg ccaggccgga atccatgagc cgagatcggc gagcagttcg tcgaagctcc
    10921 attccgcggg cggcttcgca gggcgcttcg attgcaccag cggtcggtct ttggcgacaa
    10981 tgcgatacac gcgaccttcg ttgttttgaa tcccttgctc atcaaaggtg ccgccgtagt
    11041 cgcgtcccca gccgagcacc cacaacacgc cgtcgggtcc catggcgata tcggtggggc
    11101 ggaaaagcga cttacccccg accacgaagt cctcgtagcg cccgccttgc ggcacgttga
    11161 gcgcgccgtt ccactcgggc cggtagaggt agattttttt ggtcatccag tcgccgatga
    11221 accacacgcc gcgatgcgac tcggggaaat gcggtgtgtc ggcgaagacg atgccggtgt
    11281 agctgccctc gatgatctgg ccgctgttcg gcgcggtggg caggtggcct tcgccgggcc
    11341 aggccgggct ccatggatgg ccccagccga agtgcgcgtg ttgaaagggc atgaacacgc
    11401 ggtcgccgcc ggtctggtcg ttgtcggtgc cgagccagtt gaaggccgag tcgaaggcga
    11461 tgtcccacgg attgcggtag ccgcgcgagt ggatcgtcag cgaaggcgta gcgggatcgt
    11521 aacgcaggat gccgccggtc tggccccagt cgtcggccgg gtcctggtaa gtggcgcggt
    11581 aggtctcgcg cgtgaacact tccgatggcg gcggcagatc cggtgcgccc ttcggccccg
    11641 gataacccca caactcgcgg aacgcctttg gcgcgacgcg gcccggctcc ttcggcgcat
    11701 caccatcgag actgatgccc ttcgagttgc ccttgctcag gtaaaggcag ccgtcaggac
    11761 cccagttcag gccgtgcagg caatgctcga tgttgccgag gtcggtgaag actttcacat
    11821 actcgtccgc cacgtcgtcg ccatcggtgt cgcgcaccac ggtgagatcc ggcgagttcg
    11881 ccacccacag ctcgcgtccg cgccaggcga tgctctgcac gctgttgaag ccctcggcga
    11941 agactttctt tgactccgcc acgccgtcgc catcgcgatc ttcgaggacg acgaccatgt
    12001 ccttcggtga atcgggacgt ggtgcccgcc actgcggccc catgccgacg aacaaccggc
    12061 cctttgcatc gaaagccatc gcgcagggat tcgagacgag tggctccttc gcccagagct
    12121 gcacctcaaa acctttcggc gcggtcggca gcgcgttcaa atcggcgtcc ttcgcgtcga
    12181 aagaggttcg tgcttgaagg ctgaccgccg cggccaggca tagaatgaaa aaggtgggtt
    12241 tcatttcgcc tttccctttc cttttcctgg tgccttccgc gccggcgcgg gtccgtcgaa
    12301 aaagcgcgcg ccgctgccgg tgtggtcgta gtcgccgagg tccatgcgcg cctcgtgggc
    12361 gaactggcgg aggcggtcga ggacttcggg atgttcggcc gcgacgtttc tggtttcgcc
    12421 gatgtcgttg tcgagatcgt agagcgagaa ctcattcacc gcattgccgg cgaagcgtcc
    12481 gctccagccg acccagggcg gatgcgcggg gcgtttgcga acgaacttcc acttcccgct
    12541 gcgcacggcc tggaggtggg tgaagcagta gtaaaaaaag gcttcgtgcg gggaagcggt
    12601 ggtggccgga tcgcgcaaca gcggcgtgag gtcgcggcca tcaatgacgc ggtccgacgg
    12661 cgtctttcct ccggcgatgg aggcaaaagt gggcagcaga tcaatggtgg aggtgatggc
    12721 atcgctggag gtgccggcgg ggatttttcc cggccaccag gcgacgcatg gcacacgcag
    12781 accgccttcc caggtcagca ttttgtagcc gcgcaaggga tcggcgttgc cggaatgatc
    12841 gcgcgggatg aatgccttgc cgtccggctc catgccgcgt ttcgtctcga cccaagggcc
    12901 gttgtcggag gtgaaaatca ccagcgtgtt ctccgcgagg ccgagttctc gcaaggtgtt
    12961 gatgatttcg ccgcaactcc agtccatctc ctcgaccgcg tcgccgtaaa ggccgcccgc
    13021 acttttgcct ttgaacttct ccgacgcgcc gagcgggatg tgcggcatgt tgtgcgcgag
    13081 gtagagaaag aacggcttct cacgctgctg acggatgaaa tcgagcgcct cgcgggtgta
    13141 gtcctgcgtg atgtgatccc acgactcgac cgcgggcgtg acgacctgct cattgcgcag
    13201 gatcgtgctg cggaatttgg agtcggccac gttcaccgtg gcgccattga acagcggcgt
    13261 gccgtagaag tagtcgaagc cttgtccgtt cggcatcgtg gccggatcaa acccggtggg
    13321 tgagtccttc gcatgttcgc cgaggtgcca cttgccgatg caagcggtgg cgtagcctgc
    13381 ctcgcggagg acttcggcca tcgtgacctc cttcgggtgc atgacggtgt gctggttctt
    13441 cttattgccc ggttcgccga tgcggatcgg atagcagccc gtcatcagcg ccgcccgcga
    13501 cggtccgcac accgcctgcg cataaaaact cgtcagccgc agcccttccg cggccatctt
    13561 gtcgagatgt ggtgtcttga tcttcgtgga accgaagcat ccgagatcac catagccgag
    13621 gtcatcggcg aagatgagga cgatgttcgg ctgcgccgct gcgaagagcg gggaacagag
    13681 agtaaggaga aaaatgacaa ggcgcatcat gcagcctgaa acgcgggtga aacactgaac
    13741 ttcagtccgc gatgctcatc cgacagcctg aaatccacat gccgcgtcat ggtgccgctt
    13801 ttccggcaaa ctcttcgcgg gtcactttgc catcgctgtt cttgtcgaag tttttgaagc
    13861 ggttctcgac gttgtccttc ttcgcgaggc cgttgcggta ttcttcgagc gtgaggtggc
    13921 cgtcctggtt cttgtcccag tttttgaagg cgcgcaggcg ggtctccgga gtgatttcgg
    13981 cgggcttgga aggctttgcg gctggtttgg gcgctgcttt ggcccgcagc gcgggatcga
    14041 cgtttttggg cagtgtggca aaccactcgt cgatggcggc ggtcatgcgc ttcacacgct
    14101 cggggtcggc ggcggcgagg ttgttggatt ggaaacgatc cgcgatgaca tcgtagagct
    14161 ccgcgcgctg cttcgtctcg tcgaggatga gcacatgagg gccgtcgcgc atggcaaagg
    14221 cgggccagtc atcgccgctg tggccgccct gccaccacca gaagacgggc ttcgtgcgcg
    14281 gttgcgttcc gcctttgaaa gcggcgagca tgtcctcgcc atcggaaacg tagcctgccg
    14341 gagccttcgc gccagtggcg gcgagcacgg tggggaagac atccacgccc gcgagcgcgg
    14401 tttccttgtc cacacggccc gcaggcacca caccgggcca tcgagcgagg aaggccacgt
    14461 tcacgccgcc catgagcagg ctgcgcttgc gaccacgcag gccgcccgtg ctgccctggc
    14521 tgtgataaaa cttctgcccc ggctgcgcgt ggctgttctc gggcccgttg tcgctggaga
    14581 acatgacgag cgtgttgttc gcgatgttca gttcgtccag caggtcgagc acgcggccca
    14641 cctgacggtc cgcgcgcgaa acggcggcat agtaggtgcg ctgcggctcc ggcacatcgg
    14701 ggtaaggctt tttgtcgtcc tccgtggccg agacgaggtg atgcgtctca tgcagccaga
    14761 gattgaggta aaacggagcg cccttggcct cgcggatgaa gcgcagcgcg tggtcggtgg
    14821 ccgccacgct gatccatgag gcggcttgct tgtcatgcgc tgagccttcg cctttggcga
    14881 actcatactt catgccatca aagacatgcg cccccggtcc ggtccacacg gcggcgtcat
    14941 cgtagccata ggcggagggc tgcggcgcac cttcgatgtt gccgccggag aggtgccatt
    15001 tgccaaagtg ggcggtcttg tagccggcct ccttcatcac gcgcggcagc agcggtgctt
    15061 tcggatcgag ccagtcgggc atgttccgcg ccacgttctg ctcatgcgag gcgaagtgct
    15121 gatgcacgcc ccagcgcgag ggaaactgcc ccgtgacaat gcccgtgcgg ctgggcgagc
    15181 acaccggatt caccaccgtg aagctccaga agtccgtgcc ctccttcgcc atccggtcga
    15241 ggttcggcgt ctggatgcgc ggatgcccgt ggcaggccag atcgccaaaa ccgaggtcgt
    15301 cgcagtagat gaagacgaca ttcggctgcg ccgcgaggag cgcagggcag agagcgagga
    15361 ggaaaaggca aggcttcatg gctgatagtc ggctccggtc aggcttttga gcacgctttc
    15421 ctgccaggct cgcagcttgc tttgcagcga tttcacgagg tccggttgtt tttcggcgag
    15481 gttggttttt tcagcgggat cggccttcaa gtcgaagagt tcgacttttg ccgcgccgcc
    15541 tttttgctcg tggatgacga gcttgtgatc accttcgatg atggagcgcg cgccgaggta
    15601 atcttcctcg ctgatgacgg gatggtggaa gttggtgaag tcccgtgtgg ccttgttgcc
    15661 catctttttc accagcggcg tggttccctg ctgaagctcg ggagcgatgt agggcttcat
    15721 ctcgcccttt ttccctccgg ctgggcgata ctcccaaaaa tgcagcgcac cgggccgtgc
    15781 cgtcatcttg ccatcgagca ccgctgccag gtccatgcca tcgagcggac gatccggcag
    15841 cgtctgcccc gcaatggcgg cgacggtggg cagcagatcg ctggtgctgg cgcgcacgct
    15901 ggtgctgcgc ggctccggga tgcgcgcggg ccattcaatc aggcctggaa cgagggtgcc
    15961 accctcgtaa aacgtggcct tggcgccgcg atgcggaaaa ccgagcgctg catcggggga
    16021 ggtgccgttg tcgccgcagt agaagagcag cgtgttctcg cgcaggccgt tttgcgcgag
    16081 gtgttttctc agggtgccga tggcgcggtc catggcggtg atctcggcgt agcgctcgcg
    16141 cagcacctcg ccctgcggac gtgtggtggg gccgccggtt tcgttggagg tcagcttgac
    16201 tttcttcgtg tatttggcgg gcaggtcgtc ataaagcgcc agatcctccg gcaggccgct
    16261 gtagggctcg tgcggggaac cgaaccagat gacttgtaag aagggtttgc cctctttttg
    16321 cgcgcgtccg atggaggcga tggcctcgcg gatgaggatc tcggagcttt cgcctttgaa
    16381 gacctccggc ggcccgccat tgcgcgatag tgaggggttc atctcgaaga agttgtcgtg
    16441 tgaaaggcac tcgtggaaac ccatcgccag cgggctcacg ggcgactcct tcttcaccgc
    16501 gccgacatgc catttgccga gatgggcgca gtggtagccc gccttgccca aaagatgcgc
    16561 gctggtgatc tcctccgggc ggaaggacca gcctggggca aaggttccca tgcggttcgg
    16621 gtggcgtccg gtgaggaaac tcgcgcgtgt gggcgagcag ctcggatgcg cggcgtagaa
    16681 gcgctcaaac ttcagccctg tggccgccat ctcgtcgagc acgggcgttt tgacatgcgg
    16741 atgcccgttg tagcctgtct cctcccagcc gtggtcgtcg cccatcatga ggatgatgtt
    16801 cggc
//
LOCUS       DAOMYJ010000106        16735 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Prosthecobacter sp. isolate AMR_MDS_5629
            NODE_1195_length_16735_cov_23.356475, whole genome shotgun
            sequence.
ACCESSION   DAOMYJ010000106 DAOMYJ010000000
VERSION     DAOMYJ010000106.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867850
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prosthecobacter sp. (wastewater metagenome)
  ORGANISM  Prosthecobacter sp.
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Verrucomicrobiaceae; Prosthecobacter.
REFERENCE   1  (bases 1 to 16735)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16735)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.30
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:34:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,742
            CDSs (total)                      :: 4,684
            Genes (coding)                    :: 4,676
            CDSs (with protein)               :: 4,676
            Genes (RNA)                       :: 58
            tRNAs                             :: 55
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16735
                     /organism="Prosthecobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1195_length_16735_cov_23.356475"
                     /isolate="AMR_MDS_5629"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1965333"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..196
                     /locus_tag="PLP58_12160"
     CDS             <1..196
                     /locus_tag="PLP58_12160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ09320.1"
                     /translation="LAGGGGFFAGRSHTPPTPEAMVENASKPRQQSPWAQYRMSFDPA
                     GSGVQVVRVDTDKLQNKSLR"
     gene            292..1704
                     /locus_tag="PLP58_12165"
     CDS             292..1704
                     /locus_tag="PLP58_12165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ09321.1"
                     /translation="MLAILLSGCLPEDRVWWSPNGDIAVVATGNMVQLATVDGHRSRP
                     LNLGKQGDLFKSVSWLRDGSGFVAQRTRIVAKWDELRALIPAEEIRSVETMLPLIIPM
                     LEAAERLGDETKTLEDLIASLPEGQMARFAHAARRKFEQEPERVEKLLLALPEGAKLA
                     GEFGKPESGYEISELCVFKLDGEAVTEAKALVCSLLRPALMPRMSPRHDALAFLRLDE
                     DGESAALEVIPLDGGAGQTVARLASGAFDWTPDGQSLVFMAPLGGEGEKLQSIHRVSV
                     IDGNGALANPGKPVTLATAVTLNRPVVQVLADGRVLFASQPVTLPATGTGPELSPLLF
                     LIAADGSAVTPVPTAPGDLPVNLGFFTASPDGKRVAVVESETDAVAVVEIATGKTQII
                     SPPHPSWQCRTMPAWKSATELTFAALHDSAPAWMLWREDAPLRCISESWPKSSTANWL
                     EQKKDSAAHPAPVSKLDPAR"
     gene            1701..4106
                     /locus_tag="PLP58_12170"
     CDS             1701..4106
                     /locus_tag="PLP58_12170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009961397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PSD1 and planctomycete cytochrome C
                     domain-containing protein"
                     /protein_id="HRJ09322.1"
                     /translation="MKHAILLLFTTSALANDTAFFEAKIRPVLAKHCYECHSAEAGKS
                     KGGLMLDTKHGIREGGETGPAVVPGDAAKSLLLAAIKHSDPDLEMPPKEPKLPDSVIA
                     DFESWIKAGADDPRETAAKAAERPPVDVESGKKFWAYQKPVMPKNAAQGIDGFIREKL
                     AEAGLKPAPRAEAGVLKRRLSFDLVGLPPSDGADESDLSDYEKQVDALLQSPRFGERW
                     GRHWLDVARYAESNGRESNLTFPHAWRFRDYVIDAVNADIPFDRFITEQIAGDLLPTK
                     DDAERARLLTATGLLAMGPKGLNEQNKAQFAADVADEQLDTVTRAVLASSIACARCHD
                     HKTEPFSMEDYYGLAGIFKSTQTFYGNWIDSENNNHGHLIRLPDLPGQTIVGKSLPEA
                     RVKQLQTDLAKLNAEDKEMNDYIAKAKAENRDISGEFNKLLGNALRIIWSRGGVEGAL
                     EAVDEKGRALPLCMGAKDGKIHDSALYERGEIAHPGKIVPRGFPRVMDLKIAVPKEQS
                     GRLELAKWLTSRENPLAARVMVNRVWRHLFGTGLVRTVDNFGFSGERPSHPELLDYLA
                     IRFMDGGWSVKKLVKEIVMSETYRQGSDGVMEHGIHAQNSNTPVLHDSIDPDNRLLSH
                     ANKRRLDAEVIRDSMLAVSGLLDTSRRPGSLVAELDGQSVSLIGFNTKLPTDLDGSHR
                     RSIYLPVVRDHLPDVLEQFDVANPNLVTGDRDVTNVPLQGLYLLNGPFVQEMAAALAK
                     RIESAPNRVQRAFEQCFNRRPDAQETALAEKFLQTTSADDSKLLAAFCQSLLASAEFR
                     FVD"
     gene            complement(4121..4540)
                     /locus_tag="PLP58_12175"
     CDS             complement(4121..4540)
                     /locus_tag="PLP58_12175"
                     /inference="COORDINATES: protein motif:HMM:NF013970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HRJ09323.1"
                     /translation="MFILDTNHLRELTDGTVLGEKLRQRILASKTGVVTSIISADESL
                     RGWLALIARAASPERESEAYSRLNETISILGDLVRLPYDLDAAARFQSFRKAGVRIGS
                     KDLKIACIALEYDATVLTRNTRDFAVVPGLKMENWLD"
     gene            complement(4548..4745)
                     /locus_tag="PLP58_12180"
     CDS             complement(4548..4745)
                     /locus_tag="PLP58_12180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ09324.1"
                     /translation="MKPVEMETTPLESAAERARRKQAAARELIRKSGRDGSGVFGRLP
                     DDEMTREAWALGEAWRKSEAP"
     gene            4816..6195
                     /locus_tag="PLP58_12185"
     CDS             4816..6195
                     /locus_tag="PLP58_12185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007413557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ09325.1"
                     /translation="MSIRVVIFSILAFVPSFAHAETPKPCRIEVVEKGSGWPVPLIEL
                     RTTHQMRFITDNAGVIAMDAPELMGRQIWFDVIGHGYEVPKDGFGMRGVRITPEHGET
                     LRVEVNRVNIAKRIGRITGAGLFAESQKLGSNLDWQDSGIHGSDSVQNAVHRGRLFWA
                     WGDTTMPNYPLGIFDMSSATTAIQPLTSLEPPLRLRLDYFTDDKGRQRGVAKMPGSGP
                     TWVTAYVSLPDKTGTQRLVGSYVKIKPPLDAYESGLCVWNDETSRFDLLRVLWTKSDA
                     APKQTPMPEGHPAFWKDEQGKEWVLFGNPLPTLRCPATFEAWQDSSTWEVLKPQETLA
                     STDGGKVKPHSGSIAWSEFRKRWVTVFMENFGKPSVFGELWYAEADEPTGPWSTAVKV
                     LTHENYTFYNPRIHPEFTPAGSPVLFFEGTYTKEFAKHAHATPRYDYNQILYRLDLDD
                     PGLKPATMP"
     gene            6192..7589
                     /locus_tag="PLP58_12190"
     CDS             6192..7589
                     /locus_tag="PLP58_12190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007416781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1501 domain-containing protein"
                     /protein_id="HRJ09326.1"
                     /translation="MNTHPFSRRHMLTTMSSGIGWLAFSALAQARGGLASKTPHFAAR
                     AKRVIFLTMRGGPSHVDLFDYKPELAKRDGKTATLGRDSAGAKLFGAVHPFGQYGQSG
                     QWMTTLYPKLAKHADDLCIINSMNTELPNHAQAFVQMHTGSFQFVRPSIGAWTLYGLG
                     TENENLPGFITLNPPADNGGAQNYGCAFLPAICQGTKIGGSQLPDLYAALLKKDVAPG
                     PPMKNIENKNVPRDLQRAQIDLIRGLNERKMERDGFHPEIEGAIESFELAFRMQSEVP
                     DVLDLRGEPEHILKLYGIGSGKDQFARQCLLARRMAEAGVRFIEIASGVQWDHHNRLR
                     EQLAANCAATDQPIAALLTDMKARGMLKDTLVVWAGEFGRTPYAQAGDGRDHNHKGYS
                     IWMAGGGVKGGLRYGATDEIGYKAALNPMHIHDWHATMLHLLGLDHTKLTYNYGGRNF
                     RLTNVSGEVAKGILA"
     gene            7668..9056
                     /locus_tag="PLP58_12195"
     CDS             7668..9056
                     /locus_tag="PLP58_12195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009959857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HRJ09327.1"
                     /translation="MENSSLYPTSRRDFLKTTGKTVAGLSALSGIQIPYVHAQGDDTI
                     NVALVGCGGRGTGASSNAMGVKQRTRLIAMADVQENRLEASFNGLSNKHPDRFSVSAD
                     AKFIGFDAYKQAIDMLKPGDVVVLATPPAFRWVQFKYAIEKGVNVFMEKPICVDAPSG
                     KRMLELGKQADAKGLKVGVGLMCRHCRVRGELFDRIQQGEIGDIILGRAYRLQGPVGT
                     CFTQPRNPETDPSELIYQIKNFHSFLWASGGAFSDFNIHNIDEICWMKNDFPIEAKAT
                     GGRTDRGDYVDQNFDHYSVEYTFRDGTKFWLEGRNAMKTHNEFASYVHGTKGMAVVST
                     AGHFPSKAMTFKGQNKDPKNIIWRGKQPEPNPYDLEWQDLIDAIVNGKPYSEVERGAK
                     ASLVTVMGRMSAHTGQLVTYDQLMAHDHEFAPDADKLSLDGPAPLKADANGKYPIPYP
                     GRHGMYEYVVGA"
     gene            9147..9503
                     /locus_tag="PLP58_12200"
     CDS             9147..9503
                     /locus_tag="PLP58_12200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ09328.1"
                     /translation="MNQTQREALFDLLTLSIYADSHISLTEEALIESAFIAEGWESEY
                     PKNLFIEKSFARAREAAESDAAAKDYIASRAKAFTDASSQAEAVGVVSNILSRDGLTS
                     EENRFIAALKSALPAL"
     gene            complement(9466..10386)
                     /locus_tag="PLP58_12205"
     CDS             complement(9466..10386)
                     /locus_tag="PLP58_12205"
                     /inference="COORDINATES: protein motif:HMM:NF016946.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008488 - gamma-glutamyl carboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0017187 - peptidyl-glutamic acid
                     carboxylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTTM domain-containing protein"
                     /protein_id="HRJ09329.1"
                     /translation="MKHDALKATLPGKIFLSTEDERVYAAVRISLALAALWNLVMLWP
                     LRVTLLAEEGSVPQAAAAEHSEMCTVLLFHFFPSAGGVTLLMLLAAAAMLLLLAGVLP
                     RLAAFSVFLWQMSLMERAPLGAAGWDMVLRSFSFLVLVSPLGKCWSLLAIIGRRSSVP
                     ARVARYGLVLMRLQVLVVYWQTVVLKLANPNPYWWNGEFLPYFLLSEYSRWPGAWVAE
                     YADALALGTWAALLAEAVVPVLLLIRRTRFYGMALGMILHGLITLAAPQIGPFMVVMA
                     ATYAAFLTGEDVRRIGQCLTALAARFSARR"
     gene            complement(10383..10991)
                     /locus_tag="PLP58_12210"
     CDS             complement(10383..10991)
                     /locus_tag="PLP58_12210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ09330.1"
                     /translation="MPAAAPQSNRDEPESHPPARGAGVLRDGVILVFASIHALKLLAG
                     VIPEASRPVPVPHELAHAYEALTGSRQNWTMFETIPNYHKFRVRLVITNEAGQERELG
                     PLMPGFQSWPDPAPARLFVQYDRLFPGNQSAVMREACLRRLDDELRGRGLVATGERWH
                     LETLEDFTRHLVHIGRDGRLYERRVNRHEPPPAPAGQHMEDR"
     gene            complement(11134..11502)
                     /locus_tag="PLP58_12215"
     CDS             complement(11134..11502)
                     /locus_tag="PLP58_12215"
                     /inference="COORDINATES: protein motif:HMM:NF012241.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HRJ09331.1"
                     /translation="MTTTLEKPARAEADENLLKPLYRINGNEHAYEVRVELPGVPKSG
                     VSIDLEDNILTIRGKRAAGVPDGWKTLHSEIPALDFLLRLRLNTPVDEESLKAVVENG
                     VLSVHLPVKEAARPRRIEIQ"
     gene            complement(11539..11970)
                     /locus_tag="PLP58_12220"
     CDS             complement(11539..11970)
                     /locus_tag="PLP58_12220"
                     /inference="COORDINATES: protein motif:HMM:NF012241.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HRJ09332.1"
                     /translation="MKITRFNPSLNLGRVADFDQWVRNPFAAFPLMGQLLGDFAPAFT
                     SGRPATDVHEDKDNYYARFELPGVKKEDLKVEIHDRLLNVSAERKEKNGEQESSFTLC
                     RSISVPEGVNAEGISARLEDGILVVTLPKQEHRKPKLIEIA"
     gene            12306..15692
                     /locus_tag="PLP58_12225"
     CDS             12306..15692
                     /locus_tag="PLP58_12225"
                     /inference="COORDINATES: protein motif:HMM:NF019223.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PSD1 and planctomycete cytochrome C
                     domain-containing protein"
                     /protein_id="HRJ09333.1"
                     /translation="MNKKHPWIWLGALVCASAVFSSHAAAPKKRGGVDTSSLPEPAGR
                     PVKYFGDVHPLLAEHCLSCHGADKQKGGLRLDSREAALKGGSSYGPAIIPGNSAESPF
                     IQFIAHLEPDMEMPPDEEMLTEDQIAVLRAWIDQGAKWPAKAGVAEGGESTLGNQELF
                     FKKAATHWAFQPVPVLKAAQLADGPARIDALVAAKLGKKNLARSPRADARTLMRRIHF
                     DLTGLPPAPEEVERFSAEFARDADAAVSALVDGLLASPHFGERWGRYWLDLARYADTQ
                     DFRAQQDLRYPFAWTYRDYVVTAFNADKPYDRFLQEQIAADQLGLEKDDPTLAALGFM
                     TVGPRFRGRVDEIINDRIDVVTRGLMGMTVACARCHDHKYDPIPTADFYAMYGVFAST
                     QDAEELPEIALKQAEPTPEQRADYEAKKGAAKQALEAFVQDLKDKAVADVLAKPELYF
                     DALCQMEIRKTADVRKLITGNKMMETALTPISRQWSELRRREDWQASSVLGPLARVAS
                     ASADRKAGIIEGILENGRVPGSRAEVQPALLAALRAAKPQDEESLVRTYGKVLAEAVK
                     KPEGPLGEVLAAFKDKGGWLDFPLDSVEGAYRLLGKGRADLNKLHSAVSEVEATHPGA
                     PPRAMAVADRAKPVTQAIFVRGDPQRRGDTVERRFLEVLDPSKKPYPADRSGRRELAE
                     QITARDNPLTGRVWANHVWRHLLGEPLVKTVGDFGLQAEPPSHPELLDWLAAALVDRG
                     WSTKKLIRDVMLSATYQQSSADRPEAAAVDADNTLLWRANRRRMDFEAMRDAMLATSS
                     QLQLTLGGRAESLSAEPFSGRRTLYGYVDRVNLDPLFTTFDFPSPDIASPERSETLVP
                     QQALFALNDGFIVSQARALAKNARQAAGTSESGMVDWLYRHVFYRQPTNEESALAQAF
                     LKETAGISGASARGSWLYGYGSADPAVPRAQTFKRLPYFDPATKRYQGGRVYPHPELS
                     HVSLSATGGHPGRNLANAAIRRWVAPYDGEVSIGGEFALNRQGGDGVRARIISSRQGL
                     LGEWTVGEQPAKTLVEKHSVKRGEILDFAVDCVGHPSRDAYRWAPTLQLLIRAEDAPT
                     DVQTVWDAQADFKAPPPPKLQPLEQMAHALLMTNEFLFVD"
     gene            complement(15723..>16735)
                     /locus_tag="PLP58_12230"
     CDS             complement(15723..>16735)
                     /locus_tag="PLP58_12230"
                     /inference="COORDINATES: protein motif:HMM:NF013375.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HRJ09334.1"
                     /translation="DAGGAVARLAGIYGPGRSFVLKNLLEGKSGVETAEGAPDGRFIN
                     QIHRDDAAAALARIVTQEITGVFNVVDDAPMPQRACLEKLAFLFSLPRPRDTAPDPGR
                     KRGWTHKAVSNARLRAAGWTPLYPDYFAALRDDPDLASSILQLVSEGGAASLPRAENI
                     VLIGLMGCGKTSVGRTVANMLGFQFLDTDHLIIEEAGRPIPAIFASEGEAGFRLRESA
                     ALRRLIGRRHCVIATGGGIITQARNRPLLQHLGFITWLEADPELLARRTAGNNDRPLL
                     RGGDESPLEKLRRLLAERGPLYKSLADLRIQTGELSQPETAYGVAESARIFFSGRRRL
                     AS"
BASE COUNT         3252 a         5291 c         5328 g         2864 t
ORIGIN      
        1 gctggcgggc ggcggcggat ttttcgcggg gcgcagccac acaccgccca ctcccgaggc
       61 catggtggaa aatgcgtcaa aaccgcgcca gcagagtcct tgggcacaat accgcatgtc
      121 ctttgatcca gcgggctccg gcgtgcaggt ggtgcgcgtg gacacggaca agctccaaaa
      181 caaatccctg cgatgaatgc cgccaagcgc gatttgaact caagcatggg gcacaggcat
      241 cccgcctgtg ctgaacaggc gggacgcctg ttccccatgc tctttccgcc catgcttgcc
      301 attctgctct ccggctgcct gccggaggac cgcgtttggt ggtcgccaaa cggtgatatc
      361 gcggtcgtcg ccacaggaaa catggttcaa ctcgcgacgg tggacggcca tagatcgcgt
      421 ccgctgaatc tcggaaagca gggcgatctc ttcaaaagcg tgagctggct gcgcgatggc
      481 agcggctttg tggcgcagcg cacgcgaatc gtcgccaaat gggacgaact gcgcgccttg
      541 atcccggcgg aggagattcg atccgtggaa acgatgctgc ctctcatcat ccccatgctt
      601 gaagcggcgg agcgcctggg agatgagacg aagacgcttg aagacctgat cgccagcctg
      661 ccggaggggc agatggcacg cttcgcacac gctgcgcgac ggaaattcga gcaggagcct
      721 gagcgcgtcg agaaactgct cctcgcgctg ccggaggggg cgaaactggc cggggaattc
      781 gggaaacctg agtcgggtta tgagatcagc gagctgtgcg tgttcaaact ggacggtgag
      841 gcggtgacag aggccaaagc tctcgtctgc agcttgctac ggcctgcatt gatgccgcgc
      901 atgtctccaa ggcatgacgc gctggcgttt ctgcgtctcg acgaggatgg cgaaagcgcg
      961 gcgctcgagg tcatcccact cgatggcggc gcggggcaga cggtggcgcg tctggcgagc
     1021 ggagcgtttg actggacgcc ggacggccag tcgctcgttt tcatggcacc gctcggtggc
     1081 gagggagaaa agctgcaaag catccaccgc gtcagcgtca ttgacgggaa tggagcactc
     1141 gcaaaccctg ggaagccggt cacgctggcc acggcggtca cactgaaccg ccctgtggtg
     1201 caggtgctgg cggacgggcg cgtgctcttc gccagccagc cggtcacgct gcccgcgacc
     1261 ggcacggggc cggagttgtc accgctcttg ttcctcatcg ccgccgatgg cagcgcggtg
     1321 acaccggtgc cgaccgcgcc gggcgatctg ccggtaaatc tcggcttctt caccgcctcg
     1381 ccggatggca aacgcgtcgc cgtggtggag agcgagacag acgcggtggc cgtggtcgag
     1441 atcgccaccg gcaaaacgca aatcatctcg ccaccgcatc cgtcgtggca gtgccgcacg
     1501 atgcccgcgt ggaaatcagc caccgaactg acctttgccg cgttgcacga cagcgcgccc
     1561 gcatggatgc tgtggcgcga ggacgctcct ctgcgctgca tcagtgaatc gtggccgaaa
     1621 tccagcaccg cgaactggct ggagcaaaaa aaagacagtg ccgcccatcc cgccccggtc
     1681 tccaaactcg atcccgcccg atgaaacacg cgatcctcct tctcttcacc accagcgcgc
     1741 tcgcgaacga cacggccttc tttgaggcca aaatccgtcc cgtgctcgcg aagcactgct
     1801 acgagtgcca ctccgccgag gcgggcaaat caaagggcgg gctcatgctc gacacgaagc
     1861 acggcattcg tgaaggcggc gagacagggc cggcggtggt gccgggcgac gcggccaaaa
     1921 gcctgctgct cgccgcgatc aagcacagcg accccgatct cgaaatgccg ccgaaggagc
     1981 cgaaactgcc tgattcggtc atcgcggact ttgaatcgtg gatcaaggcc ggcgcggatg
     2041 atccgcgtga aacagccgcg aaagcggcgg agcgtccgcc ggtggatgtg gagagcggaa
     2101 agaagttttg ggcctatcaa aagcccgtga tgccgaaaaa cgccgcgcag ggtatcgacg
     2161 gcttcattcg agaaaagctg gctgaggccg gtttgaagcc cgcgcctcgt gcagaagctg
     2221 gtgtgctgaa aaggaggctt tctttcgatc ttgtgggtct tccgccgtcc gacggggcag
     2281 acgagtccga cctgtcggac tatgaaaaac aagtggacgc gctcctccaa agtccgcgct
     2341 tcggcgagcg ctggggccgc cactggctgg acgtcgcgcg ctatgccgaa tcgaacggcc
     2401 gcgaatccaa cctgaccttc ccgcacgcgt ggcgcttccg cgattacgtc atcgacgcgg
     2461 tgaacgcgga cattcccttc gaccgcttca tcacggagca gatcgcagga gatctgctgc
     2521 ccaccaaaga tgacgcggaa cgcgcgcgcc tgctcacagc caccggactt ctcgccatgg
     2581 ggccgaaggg gctcaatgag cagaacaaag cgcagttcgc cgctgatgtg gccgatgaac
     2641 agctcgacac cgtgacacgc gccgtgctcg ccagcagcat cgcctgcgcg cgctgtcacg
     2701 atcacaagac ggaacccttc agcatggagg actactacgg cctcgcgggc atcttcaaaa
     2761 gcacgcagac cttttacggg aactggattg actccgaaaa caacaaccac ggccacctca
     2821 tccgcctgcc ggatttaccg gggcagacca tcgtcggcaa atcgctgccg gaggcgcgtg
     2881 tgaaacagct ccagaccgat ctggcgaagc tgaacgccga agacaaggag atgaacgact
     2941 acatcgcgaa ggccaaggcg gagaaccgcg acatcagcgg cgagttcaac aaactgctgg
     3001 gcaacgcgct gcgcatcatc tggtcgcgcg gcggcgtgga gggcgcgctg gaggcggtgg
     3061 atgaaaaggg ccgcgccctg ccgctgtgca tgggcgcgaa ggacggcaaa atccacgatt
     3121 cagccctcta cgagcgcggc gagatcgcgc atcccggcaa gatcgtgccg cgcggattcc
     3181 ctcgcgtgat ggatttgaaa atcgccgtgc ccaaggagca aagcggccgc cttgagctgg
     3241 cgaagtggct caccagccgc gaaaacccgc tcgccgcgcg tgtgatggtg aaccgcgtgt
     3301 ggcggcatct ctttggcaca ggcctggtgc gcaccgtgga caacttcggc ttcagcggcg
     3361 agcgccctag tcatcccgaa ctgctcgatt accttgcgat ccgtttcatg gacggaggct
     3421 ggtcggtgaa gaagctcgtg aaggagatcg tgatgtcgga gacttacaga caggggagtg
     3481 atggagtgat ggagcatggg attcatgccc agaactccaa cactccagta ctccacgact
     3541 ccatcgaccc cgacaaccgc ctcctctcgc acgcaaacaa gcgccggctc gatgcggagg
     3601 tgattcgtga ctcgatgctc gcagtttcag gcttgttgga cacctcgcgg cgtcccggat
     3661 cactcgtggc ggaactggat gggcagtcgg tgtcattgat cggcttcaac acgaagctgc
     3721 cgaccgatct cgacggctca catcgtcgct cgatttatct gccggtggtc cgcgatcatc
     3781 tgccggatgt tttggagcag tttgatgtgg caaatccgaa tctcgtcacg ggtgatcgcg
     3841 atgtgacgaa cgtgccgctg cagggcctgt acctgctcaa cggccccttc gtgcaggaaa
     3901 tggccgccgc gctggccaaa cgcatcgaat cagctccgaa ccgtgttcag cgtgcttttg
     3961 agcagtgttt caatcgccgt cccgatgcgc aagaaaccgc cctcgccgaa aaattcctcc
     4021 aaaccacctc cgctgacgat tccaagctgc tggcagcctt ctgccagtca ctgctggcga
     4081 gcgcggagtt tcgatttgtt gattgaacac tgaaatgaac tcaatccagc cagttttcca
     4141 ttttcaaacc tgggacaaca gcaaagtccc tcgtgttgcg tgtcagcacg gtggcgtcgt
     4201 actcaagcgc tatgcaggcg attttcagat ctttggagcc gatgcgaacg cccgctttgc
     4261 gaaaagactg gaaccgggcc gctgcatcca ggtcgtaggg aagtcgaacg aggtcgccga
     4321 gaatgctgat ggtttcgttg agtctggaat aagcctcgga ttcgcgttcc ggcgatgctg
     4381 ccctcgcaat cagcgccagc cagccacgaa ggctttcatc cgcgctgatg atcgaagtca
     4441 caacaccggt cttcgaagca aggatgcgct ggcggagctt ttcgcccagc acggtgccgt
     4501 ccgtgagctc ccggaggtga ttggtgtcca ggatgaacat gtgtcattca gggagcctct
     4561 gacttgcgcc atgcctctcc gagcgcccag gcctcacggg tcatctcgtc atctgggagc
     4621 ctgccaaaca caccgctgcc gtccctgccg ctttttcgga tcagttcccg tgcggcggcc
     4681 tgcttgcgcc tggcgcgctc ggcggcgctt tcgaggggcg ttgtctccat ttcgactggt
     4741 ttcatgctgc gaacctaacc gttgggagct gttttccaat cttattctcc cgacgccttc
     4801 cgcccacctg cccccatgag catacgcgtt gtcattttct cgattcttgc ctttgtaccg
     4861 tcgtttgcgc acgcggaaac accaaaaccg tgccgcattg aggtggtgga gaaaggcagc
     4921 ggctggccgg tgccgttgat cgaactgcgc accacgcacc agatgcgctt catcaccgac
     4981 aacgcgggcg tcatcgcgat ggacgcgccg gaactgatgg gccggcagat ttggttcgat
     5041 gtcatcggcc acggctacga ggtgccgaag gatggttttg gcatgcgtgg tgtgcggatc
     5101 acgccggagc atggcgagac gttgcgggtt gaggtgaacc gcgtgaacat cgcgaaacgc
     5161 atcggcagga tcaccggcgc gggcttgttc gcggagagcc agaaactcgg cagcaatctg
     5221 gactggcagg actcgggcat acatggttcc gacagcgtgc agaacgcggt gcatcgcggc
     5281 cgcctcttct gggcctgggg cgacacgacg atgcccaatt atccgctggg cattttcgac
     5341 atgagcagcg ccacgaccgc gatccagccg ctgacctcgc tggagccgcc gctgcgattg
     5401 aggctcgatt acttcaccga tgacaaaggc aggcagcgcg gcgtggcaaa gatgcccggc
     5461 agcggcccga catgggtcac agcgtatgtg agcctgccgg acaaaacggg cacgcagcgt
     5521 ctcgtcggca gttatgtgaa gatcaagccg ccgttggacg cctacgagtc cggattgtgc
     5581 gtgtggaatg acgaaacgag ccgctttgac ctgctgcgcg tgctttggac gaaatcggat
     5641 gccgcgccaa agcaaacgcc catgccggag gggcatcccg ccttctggaa agacgagcaa
     5701 ggcaaggagt gggtgctgtt tggcaatccg ctgccgaccc tgcgctgccc ggcgacgttc
     5761 gaggcctggc aggattcatc gacgtgggaa gtcttgaagc cgcaggagac gcttgcgtcc
     5821 acggatggcg gcaaagtcaa accacacagc ggttcgatcg cgtggagtga gtttcgcaag
     5881 cgctgggtga ccgtgttcat ggagaacttt ggcaagccgt ccgtctttgg cgagctctgg
     5941 tatgccgagg ccgatgagcc gaccgggcca tggagcacgg cggtgaaagt gctgacacac
     6001 gagaactaca ccttctacaa tccgcgcatt catcccgagt tcacgcccgc cggatcgccc
     6061 gtgctgttct tcgagggcac ctacacgaag gagttcgcca aacacgcgca cgccacgccg
     6121 cgctacgact acaaccagat cctctaccgc ctcgatctcg atgatccggg gctcaaaccc
     6181 gccacaatgc catgaacacg catcccttct cacgccgtca catgctgacc acgatgtcct
     6241 ccggcatcgg ctggctggct ttttccgcgc tggctcaagc aagaggcggt ctggcctcga
     6301 agacgccgca tttcgcggca agagcgaagc gcgtgatctt cctcacgatg cgcggcgggc
     6361 cgtcgcatgt cgatttgttc gactacaagc ccgagttggc gaagcgcgac ggcaaaacgg
     6421 cgactttggg acgtgattcg gcgggggcga agctgtttgg cgcggtgcat cctttcggcc
     6481 agtatggcca atctggtcaa tggatgacga cattgtatcc gaagctggcg aagcacgcgg
     6541 acgacctgtg catcatcaat tcgatgaaca ccgaactgcc gaatcatgcg caggcgtttg
     6601 tgcagatgca cacgggcagt ttccagttcg tgcggccttc catcggtgcg tggacactct
     6661 acggcctggg cacggagaat gagaatctgc ccggcttcat cacgctgaat ccaccggcgg
     6721 acaatggcgg cgcgcagaat tacggctgcg cgtttctgcc cgcgatctgc cagggaacga
     6781 agatcggcgg atcgcagctt cccgatttgt atgcggcgct tttgaagaag gacgtggccc
     6841 cggggccgcc gatgaaaaac atcgagaaca aaaacgtgcc gcgtgatctc cagcgcgcgc
     6901 agatcgacct catccgtggc ctgaacgagc gcaagatgga gcgcgacggc tttcatcccg
     6961 agatcgaggg tgcgattgaa tcgtttgaac tcgcgtttcg catgcagagc gaggtgcctg
     7021 acgtgctcga cctgcgcggc gagccggagc acattttgaa gctctacggc atcggcagcg
     7081 gcaaggatca attcgcacgg cagtgcctgc tggcgcgacg catggcagag gcaggtgtgc
     7141 gtttcatcga aatcgcctcc ggcgtgcaat gggatcacca caaccgcctg cgcgaacagc
     7201 tcgccgcaaa ttgcgcggca accgaccagc ccattgcagc cttgctcact gatatgaaag
     7261 ctcgcgggat gttgaaggat acgcttgtcg tctgggcggg cgaatttggg cgcacgccat
     7321 atgcgcaagc cggggacggc cgagatcaca accacaaagg ttactccatc tggatggccg
     7381 gcggcggcgt gaaaggcggt ctgcgctatg gcgcgacgga cgagatcggc tacaaggccg
     7441 ccctgaaccc catgcacatc cacgactggc acgccacaat gctgcacctg ctcggcctgg
     7501 atcacacgaa gctcacctac aactacggcg gccggaactt ccgcctcacc aacgtgagcg
     7561 gcgaagtggc gaaggggatt ctggcatgaa caggccgcct cttgtgagca gggaggggca
     7621 agttgtgctt gcgtaatcgc gttgtttttg cgcattctgc aagccttatg gaaaattcat
     7681 ccctctaccc cacgtcccga cgcgattttt tgaagaccac cggcaaaacc gtcgccggac
     7741 tttccgcgct ttcgggcatc cagatcccct atgttcacgc ccagggggat gacaccatca
     7801 atgtggccct agtgggctgc ggcggtcgcg gcaccggcgc ctccagcaat gccatgggcg
     7861 tcaaacagcg cacgcgcctc atcgccatgg ccgatgtgca ggaaaaccgc ctcgaagcca
     7921 gcttcaacgg cctcagcaac aagcaccccg accgcttctc cgtctccgct gacgccaagt
     7981 tcatcggctt cgacgcctac aagcaggcca ttgacatgct caagcccggc gacgtggtcg
     8041 tgctcgccac cccgcccgcc ttccgctggg tgcagtttaa atacgccatc gaaaaaggcg
     8101 tgaacgtctt catggaaaag cccatctgcg tagacgcccc cagcggcaag cgcatgcttg
     8161 agctgggcaa gcaggcggac gccaagggcc tcaaggtcgg cgtcggcctc atgtgccgcc
     8221 actgccgcgt gcgtggcgag ctgttcgacc gcatccagca gggcgagatc ggcgacatca
     8281 tcctcggccg cgcctaccgc ctgcaaggcc ccgtgggcac ctgcttcacc cagccgcgca
     8341 acccggagac cgaccccagc gagctgatct accagatcaa aaacttccac tccttcctct
     8401 gggcctccgg cggcgcgttc agcgacttca acatccacaa cattgacgag atctgctgga
     8461 tgaagaacga cttccccatc gaggccaagg ccaccggcgg ccgcaccgac cgcggcgact
     8521 atgtggacca gaacttcgac cactacagcg tcgagtacac cttccgcgac ggcaccaagt
     8581 tctggctcga aggccgcaac gccatgaaga cgcacaacga gttcgccagc tacgtgcatg
     8641 gcaccaaggg catggccgtg gtctccaccg ccggccactt cccctccaag gcgatgacct
     8701 tcaaaggcca gaacaaggac ccgaaaaaca tcatctggcg cggcaagcag cccgagccga
     8761 acccctacga cctggagtgg caggacctca ttgacgccat cgtcaacggc aagccctaca
     8821 gcgaggtcga gcgcggcgcc aaggccagcc ttgtcaccgt catgggccgc atgtccgccc
     8881 acaccggcca gctcgtcacc tacgaccagc tcatggcgca cgaccatgag ttcgccccgg
     8941 atgcggacaa gctcagcctc gacggccccg cgccgctgaa agccgatgcc aacggcaagt
     9001 accccatccc ctaccccggc cgccacggca tgtacgagta cgttgtcggc gcctgatgcg
     9061 ccgcttggcg cggcatggat gaggtctgcg actcattcat gccgcggccc gattttgttt
     9121 gattcagcac cacactttcc accctcatga accagacaca gcgcgaggcg ctctttgacc
     9181 ttctgactct ctccatctac gcggactcgc acatttctct gaccgaggag gcgctcatcg
     9241 aatcggcctt catcgcggaa ggctgggagt cggagtatcc gaagaatttg ttcatcgaaa
     9301 agtccttcgc ccgcgccagg gaggcggcgg agtcggacgc ggcggcaaaa gattacatcg
     9361 cctccagggc gaaagcattc accgatgcgt ccagccaggc ggaggcggtc ggagtcgtgt
     9421 ccaacatcct gtcgcgggat ggattgacgt ccgaggaaaa ccgcttcatc gccgcgctga
     9481 aaagcgcgct gccagcgctg tgaggcattg cccgatgcgc cggacatcct cccctgtgag
     9541 gaaagccgca taagtcgcgg ccatcaccac catgaacggg ccgatctgcg gcgctgccag
     9601 ggtgatcagt ccatgcagga tcatgccaag cgccatgcca taaaagcgcg tcctgcggat
     9661 gagcagcaac acaggcacga ccgcctccgc cagcaaggcg gcccaggtgc ccagagccag
     9721 cgcgtccgcg tattccgcca cccaggctcc aggccagcgc gagtattccg acaggaggaa
     9781 gtagggcaga aactcgccgt tccaccaata tggattgggg ttcgccagct tgagcaccac
     9841 ggtctgccag taaaccacca gcacctgcag gcgcatgagc accaggccgt agcgcgcgac
     9901 ccgcgccgga acgctgctcc gccgcccgat aatcgccagc agggaccagc atttccccag
     9961 agggctgacc agcaccagga agctgaagga acgcagcacc atgtcccacc ccgccgcccc
    10021 cagcggcgcg cgctccatga gggacatctg ccagagaaaa acagaaaacg ccgccaacct
    10081 cggcagcacg cctgccagca gcagcagcat ggcggcggca gccagcagca tgagcagggt
    10141 cactccgccg gcggatggaa aaaaatgaaa gagcagcacg gtgcacattt cgctatgctc
    10201 cgctgcggcc gcctgcggca cagaaccctc ctcggcgagc agtgtgacgc gcaaaggcca
    10261 gagcatgacc agattccaca acgccgcgag ggccaggctg atgcgcacgg cggcgtaaac
    10321 gcgctcatcc tcggtggaga ggaagatttt ccccggcagg gtggctttga gcgcgtcgtg
    10381 cttcatcggt cctccatgtg ctgcccggct ggcgcgggcg gcggctcatg acgattcacg
    10441 cgcctttcat aaagacgacc gtccctgccg atatgcacga gatgccgggt gaagtcctca
    10501 agggtttcca gatgccaacg ctcgccggta gcgacgagtc cgcgcccgcg caattcgtcg
    10561 tccagccggc gcaggcacgc ctcgcgcatg accgcggact ggttgccggg aaaaagccga
    10621 tcatactgga caaagagacg cgcgggggcc gggtcgggcc atgactgaaa gcccggcatc
    10681 aacggaccaa gttcgcgctc ctgcccggcc tcgttcgtga tgacgagtcg aacccgaaat
    10741 ttgtgatagt tgggaattgt ctcgaacatc gtccaatttt gccggctgcc agtcagcgcc
    10801 tcataagcgt gggccagctc gtgcggcacg gggacgggcc gcgacgcctc gggaatgacc
    10861 cccgccagca gcttcagcgc atggatggag gcaaaaacga ggatgacccc atcccggagc
    10921 acccccgcgc cgcgcgcggg aggatggctc tccggctcat cgcggttgga ttggggagct
    10981 gcggcgggca tgacgcgcca tggaagccca tgcacaaggg tttcgtcaag caggcaacgc
    11041 acccggaacc tgtttgcagg cggcatcgcg ggatgaaaaa tggcgcggca ctcctgcgag
    11101 cgccgcgcca tgcggatcag gcaaccgtgt agattattga atctcaatcc gccgcggtct
    11161 ggcggcctcc ttgaccggga gatgcacgct gaggacgccg ttttccacga ctgccttcag
    11221 gctctcctcg tccacgggtg tgttcagacg cagacgcagg aggaagtcga gggccggaat
    11281 ctcgctgtgc aaggtcttcc agccgtcggg cactccggcg gcgcgtttgc cgcggatggt
    11341 gagaatgttg tcctccaggt caatcgagac gccggacttc ggtactccgg gcagttcgac
    11401 gcgtacttca taggcgtgct cgttgccgtt gatgcggtaa agcggcttga gcaggttttc
    11461 gtccgcttcg gcgcgggcgg gtttttcaag ggtggtggtc atggcttgga tgggctggat
    11521 gttcgcggaa acagatgctc aggcgatttc gatgagcttg ggtttgcggt gctcctgctt
    11581 cggcagggtc acgacgagga tgccgtcctc gagcctggct gagatgccct cggcattgac
    11641 gccttccggc accgagatcg agcggcagag tgtgaaggac gactcctgct cgccgttttt
    11701 ctccttgcgc tcggcgctga cattgagcag gcggtcgtgg atttcgacct tgaggtcctc
    11761 cttcttcacg ccaggcagtt cgaaacgggc atagtagttg tccttgtcct catggacatc
    11821 cgtggcggga cggcccgagg tgaaagcggg agcgaagtct cccagcagtt gccccatcag
    11881 cgggaaggcg gcaaaaggat tgcgcaccca ctggtcgaag tcggcgacgc ggccaagatt
    11941 aagcgatgga ttgaagcgtg tgattttcat aagagttgaa tttgggtttg aggttagtca
    12001 gatgacattt tcatattcct gcacgggcca tgccaaaggc acctcaaggc ttaaaactct
    12061 ctcgaaatca aaggattggg ttgattgacc gaaaatggac ccggggattt tttgggaatg
    12121 aatgacacaa aaaagaaacc aatttttcac ttccaaaaga cgtcttgtct caacttcaaa
    12181 tgaagttcca tttgggaaga cgacccggag tttaaatgca gcagcaggcg ggcggagact
    12241 cgctttgcag gtgcgaagcg ccgtgcaata cccgacgcat gaccgcgttt ccgggggtaa
    12301 accccatgaa caagaagcat ccctggatct ggctgggcgc gctggtgtgc gcctcggctg
    12361 ttttttcctc ccacgcggcc gcgccgaaga agcgcggcgg cgtggacacc tcgtccctgc
    12421 cggaaccggc gggcagaccg gtgaagtatt tcggcgacgt gcatccgctg ctggcggagc
    12481 actgcctttc ctgccacggc gcggacaagc aaaagggcgg gctgcgcctg gactcgcggg
    12541 aagcggcgct gaagggcggc agctcttacg ggccggccat catccccggc aacagcgcgg
    12601 agagcccgtt catccagttc atcgcgcacc tggagccgga catggagatg ccgccggatg
    12661 aggagatgct cacggaggac cagatcgccg tgttgcgcgc gtggattgac cagggcgcga
    12721 agtggccggc caaggcgggc gtggcggagg gcggggagtc cacgctggga aatcaggagc
    12781 tgtttttcaa gaaggctgcc acgcattggg ccttccagcc ggtgccggtg ctgaaggcgg
    12841 cgcagctcgc ggatggcccg gcgcgcattg acgcgctggt ggccgcgaag ctgggtaaaa
    12901 aaaatctggc gcgctctccc cgcgccgatg ctcgcacctt gatgcggcgc attcattttg
    12961 acctcaccgg cctgccgccc gcgccggagg aggtggagcg tttcagcgcc gagttcgcac
    13021 gggacgcgga cgccgccgtg tcggcgctgg tggacgggct actggcctcc ccgcacttcg
    13081 gcgagcgctg gggccgctac tggctggacc tggcgcgcta cgcggacacg caggacttcc
    13141 gcgcgcagca ggatctgcgc tatcccttcg cgtggacgta tcgcgactat gtggtgaccg
    13201 ccttcaatgc tgacaagccg tacgaccgct tcctccagga gcagatcgcg gcggatcagc
    13261 tcggcctgga gaaggacgat ccgacactgg cggcgctcgg attcatgacg gtggggccgc
    13321 gcttccgggg gcgggtggat gagatcatca atgaccgcat tgacgtggtg acgcgcggcc
    13381 tcatgggcat gacggtggcc tgcgcgcgct gccatgacca caaatatgac cccatcccga
    13441 cggcggattt ttatgcaatg tatggcgtct tcgccagcac gcaggatgcc gaggagctgc
    13501 cggagatcgc gctcaagcag gcggagccga cgccggagca gcgcgcggat tacgaggcga
    13561 aaaaaggcgc ggccaagcag gcgctggagg cctttgtgca ggacctgaag gacaaggccg
    13621 tggcggatgt gctggcgaag ccggaactct atttcgacgc gctctgccag atggagatcc
    13681 gcaagacggc ggacgtgcgc aagctcatca ccggcaataa aatgatggag accgcgctca
    13741 cacccatcag ccgccaatgg agcgaactcc gccggcggga ggactggcag gccagttcgg
    13801 tgctcggccc gctggcgcga gtggcgagcg cctcggcgga caggaaggcg ggcatcattg
    13861 agggcatttt ggaaaatggc cgcgtgcccg gcagccgcgc cgaagtgcag cccgcgctgc
    13921 tcgccgccct tcgcgcggcc aagccgcagg atgaggagtc gctcgtgagg acctacggca
    13981 aggtgctggc cgaggccgtg aagaaaccag agggaccgct cggcgaagtg ctcgcggcat
    14041 tcaaagacaa gggcggctgg ctggacttcc cgctcgactc cgtggagggc gcttaccgcc
    14101 tgctgggcaa gggccgcgcg gatttgaaca agctgcactc cgccgtctcc gaagtggagg
    14161 ccacgcatcc cggagcacct ccgcgcgcca tggccgtggc cgaccgcgcc aagcccgtga
    14221 cgcaggccat tttcgtgcgc ggtgatccgc agcgcagagg cgacaccgtg gagcgccgct
    14281 tcctggaggt gctcgatccc tccaaaaagc cctaccccgc cgaccgcagc ggccgccgcg
    14341 agctggcgga gcagatcacc gcgcgggaca atccgctcac cggccgcgtg tgggccaacc
    14401 atgtgtggcg ccacctgctg ggcgagccgc tggtaaaaac cgtgggagac ttcggcctgc
    14461 aagccgagcc gcccagccac cccgagctgc tcgactggct ggccgccgcg ctcgtggacc
    14521 gcggctggtc cacgaagaag ctgatccggg atgtgatgct ctccgccacc taccagcaaa
    14581 gcagcgccga ccggcccgag gccgccgccg tggatgccga caacaccctg ctctggcgcg
    14641 ccaaccgccg ccgcatggac ttcgaggcca tgcgggatgc catgctggcc accagcagcc
    14701 agctccagct cacactcgga ggccgcgccg agagcctctc cgccgagccc ttcagcggca
    14761 ggcgcacgct ttacggttat gtggaccgcg tgaatctcga cccgcttttc accaccttcg
    14821 acttcccctc gccggacatc gccagccccg agcgctccga gaccctggtg ccgcagcagg
    14881 cgctcttcgc cctcaatgac ggcttcatcg tcagccaggc gcgtgctttg gcaaagaatg
    14941 cccggcaagc cgcaggcacc tcggaaagcg gcatggtgga ctggctctac cgtcatgtgt
    15001 tttaccgcca gcccactaac gaggagtcgg cgctggcgca ggcctttttg aaagagacag
    15061 ccgggatctc aggagcctcc gctcgcggct cctggctcta tggctatggc agcgccgatc
    15121 ccgccgtgcc gcgcgcgcag accttcaagc gcctgccgta tttcgatccc gccacgaagc
    15181 gctaccaagg cggccgcgtc tatccgcacc ccgagctcag ccatgtgagc ctgagcgcca
    15241 ccggggggca tccgggccgg aatctcgcca acgccgccat ccgccgctgg gtggctccct
    15301 atgatggcga ggtgtccatc ggcggcgagt tcgcccttaa ccgccagggt ggagatggcg
    15361 tgcgcgcgcg catcatttcc tcgcgccagg gtttgttagg cgagtggacc gtgggtgagc
    15421 agcccgcgaa gacgctcgtg gagaagcaca gcgtgaagcg cggggaaatc ctcgacttcg
    15481 ccgtggactg cgtgggccac ccgagccgcg acgcctaccg ctgggcaccg acattgcagc
    15541 tcctcatccg cgccgaggac gcccccaccg acgtgcagac cgtctgggac gcccaggccg
    15601 acttcaaagc cccgcccccg ccgaaattgc agcccctgga gcaaatggcg cacgcgctgc
    15661 tgatgacgaa cgagtttctt tttgtggact gaagctcttg gcccgaagcg ctttccgcat
    15721 tctcatgatg ccagacgccg gcgcccgctg aaaaagatgc gcgcactttc ggccacgccg
    15781 taggctgtct ccggctggct gagctcgcca gtctggatgc gcaggtcggc cagcgacttg
    15841 taaagcggtc cgcgctcggc gagcaggcgg cgcagctttt ccagcggcga ctcatccccg
    15901 ccgcgaagca gcgggcggtc attgttgccg gcagtgcggc gggcgaggag ttcggggtcg
    15961 gcctccagcc aggtgatgaa gcccaagtgc tgaagcaggg ggcggttccg cgcctgggtg
    16021 atgatgccgc cgccggtggc gatgacgcag tgccgccgcc cgatgagccg gcgcagcgca
    16081 gcggactcgc gcaatcggaa accagcctcg ccctcgctgg cgaagatggc ggggatgggg
    16141 cggccggcct cctcgatgat gagatgatcc gtgtcgagaa actgaaagcc gagcatgttc
    16201 gcgacggtgc gcccgacgga ggtcttgccg cagcccatga ggccgatgag gacgatgttt
    16261 tccgcgcgcg gcagactggc cgcgccgccc tcgctcacga gctggaggat gctggaggcg
    16321 aggtcgggat cgtcgcgcag ggcggcgaag tagtcggggt aaagcggcgt ccagccggcg
    16381 gcgcgcaggc gcgcgttgga gacggctttg tgcgtccagc cgcgcttgcg tccagggtcg
    16441 ggcgcggtgt cgcggggacg cggcaggctg aaaagaaagg ccagtttttc caggcaagcg
    16501 cgctgcggca tgggcgcgtc atccaccacg ttgaagacgc ccgtgatctc ctgcgtgacg
    16561 atgcgggcga gcgcggcggc ggcatcgtcg cggtggattt gattgatgaa gcgcccgtcg
    16621 ggcgcgccct ccgcggtctc gacgccggat ttgccctcca gcaggttttt caaaacgaag
    16681 gagcgccccg gcccgtaaat gcccgccagc cgcgccaccg caccacccgc gtcca
//
LOCUS       DAOMYJ010000107        16705 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Prosthecobacter sp. isolate AMR_MDS_5629
            NODE_1199_length_16705_cov_20.504985, whole genome shotgun
            sequence.
ACCESSION   DAOMYJ010000107 DAOMYJ010000000
VERSION     DAOMYJ010000107.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867850
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prosthecobacter sp. (wastewater metagenome)
  ORGANISM  Prosthecobacter sp.
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Verrucomicrobiaceae; Prosthecobacter.
REFERENCE   1  (bases 1 to 16705)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16705)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.30
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:34:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,742
            CDSs (total)                      :: 4,684
            Genes (coding)                    :: 4,676
            CDSs (with protein)               :: 4,676
            Genes (RNA)                       :: 58
            tRNAs                             :: 55
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16705
                     /organism="Prosthecobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1199_length_16705_cov_20.504985"
                     /isolate="AMR_MDS_5629"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1965333"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..630)
                     /locus_tag="PLP58_12235"
     CDS             complement(<1..630)
                     /locus_tag="PLP58_12235"
                     /inference="COORDINATES: protein motif:HMM:NF024867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HRJ09335.1"
                     /translation="MLTELFLRDFRCFTECRLEPHPGLTLLVGRNAQGKTSLLEAACV
                     LLRLQSPRTSARGDLIRLGASTCLVEGVLGGLRLRCAQNTTARRLAVDGAVCGRSGDY
                     LEQSGRVVWMDHRDMNLLRGGGEHRRRFLDFAASQLYPDYLKALRGYERALRGRNHVL
                     KKDAVISWRQADAFARVMAGFAGTLVERRAQLVAALRPQRRTFVAWLSDL"
     gene            684..1973
                     /gene="hisS"
                     /locus_tag="PLP58_12240"
     CDS             684..1973
                     /gene="hisS"
                     /locus_tag="PLP58_12240"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018969008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004821 - histidine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006427 - histidyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HRJ09336.1"
                     /translation="MASFRTVKGFRDLFPQECALRNHITETWRAVARRYGFIEYEAPL
                     IESTDLYKKKSGDEITAQLFCFEDKGGRDISLRPEVTPSLARMAAARQRDFKKPLKWF
                     EMGSCFRYEEPQEGRTREFIQFNADILGDASPGADAELIALAVDVMLEFGLRKEDFLI
                     RLSNREIWNQYLKEQGISEEHSAAFLQIVDKIERAKPEETAAKLAAIGLSLDHVRAFM
                     RATDENHPAFAALRADLTARGLWEHVKIDASIVRGLAYYTGTVFEVFDLKHGLRAVAG
                     GGRYDKLCALISDGAVDMPAAGFAMGDVVLGILLSKTPSAQCRITDYLNAQHGVDAYV
                     VVADEAQRAPALAAVQALRRAGARVDFSMAAQKVGKQFQAAENARARLALVFGAEYPQ
                     VIVKDLVSREQGEVPAGSLAAHVLAALQEPLRGPLIA"
     gene            2062..3234
                     /gene="tyrS"
                     /locus_tag="PLP58_12245"
     CDS             2062..3234
                     /gene="tyrS"
                     /locus_tag="PLP58_12245"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012419199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004831 - tyrosine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006437 - tyrosyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HRJ09337.1"
                     /translation="MLPVTEQLEILKRGTVTIHSEKELAEKLARGKPLRVKLGVDPTS
                     PDIHLGHAVALRKLRQFQELGHQIVLIIGDFTAMIGDPSGRSTTRPPLTYEDVVANAK
                     TYTDQAFLVLDKEKTEVVFNGQWFKEMPFMEVIKLNARVTLQQMLQREDFKNRVANGT
                     EVRLHEIQYPIMQGWDSVVVRADVEIGGSDQLFNILVGRDLQKEEGQEPQVCMTLPLL
                     EGLDGVKKMSKSLGNYVGLTDAPKDMFGKLMSIPDELMAKYYLLVLGETMDAAMHPME
                     AKKQLADKCVAVYHGGEAARECREDWDIRFSKRDLSSAELPSFTPGERRDVLGVVQHA
                     FQSVFGQAVSGGDVRRLIQGGSIQLNGDKLTDPKAEPAWESGAVLKLDKKRSVRLA"
     gene            complement(3288..4220)
                     /locus_tag="PLP58_12250"
     CDS             complement(3288..4220)
                     /locus_tag="PLP58_12250"
                     /inference="COORDINATES: protein motif:HMM:NF014375.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin B12-binding domain-containing protein"
                     /protein_id="HRJ09338.1"
                     /translation="MSSTIQVASLRSGVTPHVIRIWERRYNALCPERTCTNRRLYCDE
                     DIHRLSLLRQLTECGHRIGGIARLSTAELESQLEKSRSRPVAAAASFTVDPALESPAE
                     YVRACLDAARAYDSERLHRLLQRARIQFGQRCCVHQIICPLIHEIGDAWQLGSLRPGH
                     EHIATAVVREFLMTPVPGSQVAASAPEIVVATPAGEVHELGALLAAVSARDLGWRVTY
                     LGPNLPTEEIAACARNRCARAVALSVVYPMHCPVIQEKLRKIRGLLPESIALIVGGRA
                     AESYSRQIQDPGILWAHDLPTLDQLLVRTASLSR"
     gene            4342..5736
                     /locus_tag="PLP58_12255"
     CDS             4342..5736
                     /locus_tag="PLP58_12255"
                     /inference="COORDINATES: protein motif:HMM:NF019292.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside recognition domain-containing
                     protein"
                     /protein_id="HRJ09339.1"
                     /translation="MLNHIWALLLLTGLVVAGWFGRFSGEAGVTASALAITRTAVMDI
                     ALPLAGMMMFWLGVMRLMEKAGLLELAARAVAPMMRRLFPDVPAGHPAMSSMIMNLAA
                     NMLGLGNSATPLGLKAMTHLQELNPRKQTASNAMVTFLAMNTAAFTLLPSSVINYLTA
                     AGVREAHQVIVPTILATACTTLTAILMARALQGLPVFRAAPDEGDAAAEAAEGSAAAP
                     QPAGRKTRWLVVTLAVLFGGGLLLEFSPPQARERVLETTGLSRVMERAGQKAAARQAP
                     APVEKAEEPPAWRRLMNAGSGAAIPLLLVVTLGVALTRGVKVYEEFVEGAKEGFAMAT
                     RIMPFLVAMLVALAVFRSSGALLILEMILAPVLAFAGFPMDLLPLALMRPLSGSGSVG
                     VLNEILTLPEAGETLKFTAAIMFGSTETTFYVLAVYFGSVGIRKIRHAILAGLAADAV
                     GIIMSVVIGRLMFG"
     gene            complement(5774..7879)
                     /locus_tag="PLP58_12260"
     CDS             complement(5774..7879)
                     /locus_tag="PLP58_12260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007415434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HRJ09340.1"
                     /translation="MRALLILCLMSALSSHAQTIAYPGTLRQDVTDQYHGVKIPDPYR
                     WLEDDNSEETKAWVKAQNAVTFDFLKSIPRRGDIRARLEKLWNYERFGVPWEEGGRWF
                     LTRNSGLQNQGVLLTADSPDAEPRVLLDPNTLSEDGTTSLTVTAPSRDARFLVYGLSK
                     AGSDWQEFRVKDIATGRDGPDVLEWIKFSGASWARDGSGFYYSRYPRPKEGAALTEAN
                     KNQKVYFHKLGTPQSADLLVYERPDQPDWGLHARVTDDGAYLILTVTHGTDPKNRVFY
                     RSLKDKDAPVVELLADFDADYSFLDNAGALFYFRTDLDAPRYRVIAIDTSRPQRVHWR
                     EIVPQQEDKLEGASIVGGQLLCEYLKDARSEMRAHDLDGALIRSIELPGIGTVGGFGG
                     RMDQKQIFYAFTSFTEPGAIYRYDVETGRSFLHRRPRVDFDPALYETRQIFAASKDGT
                     RVPMFIVHKKGVKLDGQNPCLLYGYGGFNISLTPAFSVSRVVWLEMGGIYAQANLRGG
                     GEYGAEWHQSGTRLRKQNVFDDFIACAEWLQKEKFTSPAKLAIQGGSNGGLLVGACMT
                     QRPELFGAALPSVGVMDMLRFHLFTIGWAWKSDYGSSENEAEFKALRAYSPLHNLKPG
                     TRYPATLVKTADHDDRVVPAHSFKFAARLQECQAKDGPPVLIRIETSAGHGAGTALKK
                     VIEETADTYAFLLRVLGMK"
     gene            8167..8538
                     /locus_tag="PLP58_12265"
     CDS             8167..8538
                     /locus_tag="PLP58_12265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009963260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymer-forming cytoskeletal protein"
                     /protein_id="HRJ09341.1"
                     /translation="MTSSKNILANDVEIKGSIKFSHDLIIDGKIEGEVHSDGSLTVGE
                     NALIKGEIKTRSVILFGKVEGNISVSERCELKSNAILVGDIEAGTLSIEEGATFMGAS
                     KVGRKPEPKPAPAGVNRPPQP"
     gene            8555..9709
                     /locus_tag="PLP58_12270"
     CDS             8555..9709
                     /locus_tag="PLP58_12270"
                     /inference="COORDINATES: protein motif:HMM:NF016408.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymer-forming cytoskeletal protein"
                     /protein_id="HRJ09342.1"
                     /translation="MFTGLFGRKSDRPPPPKNQIEVECPACGAYQYEPKMVVSTFCRK
                     CGVHLTVTKRGVTASNATRSGVGMPDPWAKEAAHAPKPPEKPASSPATEAALVMDETA
                     GELRQPVSEDEAAEGGFGVFLKQQLAGAQPATTGHNGSHAPAQDEPAAEAELVPADEA
                     EEGSGADGAAVKRPQSNSPPPTPAPPPMNEGTLQKMRDAGIYRNHYFKDAHCFECGHK
                     FKVNRSLRAVTCANCGAAIPMEDVEINMLTDRPIKTRGDVIIRKRGHVRAEYIQCREL
                     RCFGTFESNVQAEGDVVFRANGRITGRIECRRLIIEKGSDVSLLNEARAAEIEVHAKV
                     AGSLVSTGRLLIGANGSVDGDVTARSVSIEPGGELNGAMNILSGVIKNAD"
     gene            9775..10392
                     /locus_tag="PLP58_12275"
     CDS             9775..10392
                     /locus_tag="PLP58_12275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018969584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1287 domain-containing protein"
                     /protein_id="HRJ09343.1"
                     /translation="MIRFLTLLLLLASCHASQEEQAAASRARLPDDGSFASRLCLAAL
                     ERTRHQVRYDPAYVRIPYPGGDVPADTGVCTDVVVRSYRALGIDLQKEVHEDMRRAFS
                     AYPKNWGLTAPDTNIDHRRVPNLQTFLKRRGAALPVTQNAADYLPGDLVTCTVAGRLP
                     HIAIVVPAPDDGPEPWIVHNIGQGPRLEKRLFEFPITGHFRWRGK"
     gene            complement(10503..15554)
                     /locus_tag="PLP58_12280"
     CDS             complement(10503..15554)
                     /locus_tag="PLP58_12280"
                     /inference="COORDINATES: protein motif:HMM:NF020390.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1800 family protein"
                     /protein_id="HRJ09344.1"
                     /translation="MRLLSCLCLLLPAALRAEFATVWTLGVHDGTPNGFGSVWGIFPS
                     PGSASVLDDDYYFAGDYSDPVGVVAAAEPVNQADNALASGDNSASQRYHFMLSAGQAA
                     GAARVRFILHHCWGGTPADRGYGTHEFTVKLNGAVIGTRTASTYGALVVEADATPNTG
                     ANTIEVIRSGGSADGWVHIDALAFEIDPTATLDADSDGLPRWWEETHGLSDTNPADAA
                     LDLDGDDLTHLQEFARGTNPRLADTDGDGLSDAVETNTGVYAGISELGTNPLLADTDG
                     DTLSDGDEWLTHHTSPLLADSDADGAPDAWELRTGHDAMSAASTPPVFPDAVAVNFVS
                     DVNPHNALPWLAPAGLSPQLHWNNTMPLTSWNTTSGNTMHIESPQQGVLVNSAGAPTT
                     MTLAWTSPNHVWINGNTGAPDRMIMDAYLVVSDSTPASVTIGGIPFAGYDVILYAGGS
                     YDGAVGYARLNDLPATDRWFITGSRPPETRFTEPHGSTPAKPWRGNAIRWRNVTGATA
                     SVKLFPHDNHELGIHGIQIVDSATDSDGDGMTDSWELTHRLRPDLNDAALDPDGDGLS
                     NSGEYARGTDPHNPDTDGDGLTDNVERNTGVFFSHLNTGSNPLIADTDGDGLSDGEEL
                     ARLPSPTNPLLADTDSDGMDDAEEIRLGRDPLFAESAGAFLPQVTTSPRTFLWQVENV
                     QIIRDHTRGHHSGLQWGDDTLLRLTLRNSAFPNQDAFWVALRVVSGRITRYFYSNYES
                     AFSHPDNNSWDNWESDWNEPPTDLAPALGFSGHGSVDMSDRLRFRIEGSSPGSRTNWT
                     FTFSIFNQDTNTTVASTTFASCALALSVHNNSATWQDQSGAPGIYSLYQNDGVRVFIH
                     SARLDTTTAFAAWKDSDNDGMTDAWENLHGFDKNNPADASLDADNDGLTNLQEFLAGT
                     NPHNSDSDNDGAPDGVEVAAGSDPRLGSSLPPWYHGPPAGVSGADLNGNGIPDAWELW
                     LSRFDLAALDDDDGDGMSNADEAAAGTDPFDPYSRLWVDTTRAGSDLVVAWPLLALKH
                     HRLWQNDSLSPATWTPAPGVPLAFGNEYRQTVAGALTGAQPRFFKATVADLDTDGDGV
                     SDWAEVSVLGSDRLAADSARAPAPVDSNDDGQPDTALSGDHATLLEKLHGASPAGGFP
                     GGAGGGISRSQAARFLTQASFGPTLEDIEQVQSLGFEGWINAQRAASPTLHSDYIRSI
                     WQDMMTQRANPNYVRSEVVAGEDRDPFLFGGNMKTAFARAAIQGPDQLRQRVAFALSQ
                     ILVTSRRDANLENRCLGMADYYDIFVRHAFGSYHDILMEVTLHPVMGRYLSHVGNQKA
                     RPEINQYPDENYAREVMQLFTIGLWRLNPDGTRQLDSQGQPIPTYSNAEITQLARVLT
                     GLWFGGHYWGGGGWTETDYATPMTMHADRHDFGKKTLLDGFVIPARTPTHAEAMRDIQ
                     DAIRHLFEHPNTAPFVSRQLIQFLVTDNPTPAYVARVATVFTSDDYGQRGNLFSVVRA
                     ILLDPEARGPSRPADYGRLKEPVIRAIALGRAFGMKQVPDLLWWDWNDFYNDSRQEPG
                     YSPSVFNFYRPDYRAPGLLTQQGKAGPVFQITDSYSSISFPNRLWQMVMEGFSYWEEY
                     RFPIDLSRERSLAAQPERLVDHLNTLFCAGEMTRGTRALILQAVNAIPAAETAARARV
                     AAYLALVCPEGAVMR"
     gene            15723..>16705
                     /locus_tag="PLP58_12285"
     CDS             15723..>16705
                     /locus_tag="PLP58_12285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007414351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HRJ09345.1"
                     /translation="MPAPASPSFPDFRADFPILGQQVHGHPLIYFDNAATSQKPRQVI
                     EALTRYYERDNANVHRGLHELSMRATDAFEAARARAARFIGAASPDEIIFTRGTTEGV
                     NLAAQAWCARHLQPGDVILTTGMEHHSNLVPWQLAAQCHQASLRHIPVLDDGTLDMDA
                     LDSLLDGPVRLLAFVHVSNSLGTINPAREICARARALGITTFLDGAQAAGHMPVDVRG
                     IGCDFYAFSGHKMCAPTGIGVLYARREVLEEMPPWQGGGEMILSVALDSSTYKAPPTR
                     FEAGTPDIAGAIGLAAAMDYLDGIGLEAIQAHGEALTRRAWDGLSALPGIRL"
BASE COUNT         2875 a         5304 c         5659 g         2867 t
ORIGIN      
        1 caggtcggac agccaggcga caaaggtgcg gcgttggggg cgcagggcgg ccaccaactg
       61 cgcgcgtctt tccaccaggg tgccggcaaa gccggccatc acccgcgcga aggcgtccgc
      121 ctgccgccag gagatgaccg cgtctttttt gagcacatga ttccggccgc gcagcgcgcg
      181 ctcgtagccg cgcagggcct tcaaataatc ggggtaaagc tggctggcgg cgaagtcgag
      241 gaagcgccgc cggtgctcgc cgccgccgcg cagcagattc atgtcgcggt ggtccatcca
      301 caccacgcgc ccgctttgct ccagatagtc gcccgagcgc ccgcacaccg cgccgtccac
      361 ggcaagacgg cgcgcggtgg tgttttgcgc gcagcggagg cgcaggccgc cgagcacgcc
      421 ctccaccagg caggtggagg ctcccaggcg gatgaggtct ccgcgggcgg aggtgcgcgg
      481 ggattgcagg cgcagcagca cgcatgcggc ctccaggagg gaggtcttgc cctgcgcgtt
      541 gcgccctacg agcagggtca gcccgggatg cggctccaga cggcactccg tgaagcagcg
      601 gaagtcacgc agaaagagct cggtgagcat ctggaaaaac ggttgaactc gcaagcgcgg
      661 ggggcatctg tcccgccctt ctaatggcct ccttccgcac tgtcaaaggc ttccgcgacc
      721 tcttccccca ggagtgcgcg ctgcgcaacc acatcactga aacctggcgc gccgtggcgc
      781 gccgttacgg cttcatcgag tatgaggcgc cgctcattga gagcaccgac ctctacaaga
      841 agaagagcgg cgacgagatc accgcgcagc ttttctgctt cgaggacaaa ggcgggcggg
      901 acatttccct gcgcccggag gtgacgccct cgctcgcgcg catggccgcg gcaaggcagc
      961 gcgactttaa aaagccgctc aagtggttcg agatgggctc ctgcttccgc tacgaggagc
     1021 cgcaggaggg ccgcacgcgc gagttcatcc agttcaatgc cgacatcctt ggcgacgcct
     1081 cgcccggagc cgacgcggag ctcatcgcgc tcgccgtgga cgtgatgttg gagttcggtc
     1141 tgaggaagga ggacttcctc atccgcctca gcaaccgcga aatatggaac cagtatctca
     1201 aggagcaggg catctccgag gagcacagcg ccgccttcct ccagatcgtg gacaagatcg
     1261 agcgagcgaa gcccgaggaa accgcggcga agctggcagc catcggcctc agcctcgacc
     1321 atgtgcgcgc cttcatgcgg gccacggatg aaaaccatcc cgcctttgcc gccctgcgcg
     1381 ccgacctcac cgcgcgcggc ctgtgggagc atgtgaaaat agacgcctcg atcgtgcgcg
     1441 ggctggccta ctacacaggc accgtcttcg aggtcttcga cctcaaacac ggcctgcgcg
     1501 ccgtggcggg aggtggacgc tatgacaagc tctgcgcgct gatcagtgac ggcgcggtgg
     1561 acatgccggc tgccggtttt gccatggggg atgtggtgct cggcattctg ctctcgaaaa
     1621 caccctccgc ccagtgccgc atcaccgact atctcaacgc ccagcatggc gtggacgcct
     1681 atgtcgttgt cgccgatgaa gcgcagcgcg cccccgcgct cgccgccgtg caggctctgc
     1741 gccgcgcggg agcgcgtgtg gacttcagca tggccgcgca aaaagtaggc aagcaattcc
     1801 aggccgcaga gaacgcccgc gcccgtctcg ctctcgtctt cggcgccgag tatcctcaag
     1861 tcatcgtgaa agacctcgtc agccgcgagc agggcgaggt gcccgccggc tcccttgccg
     1921 ctcatgtttt ggccgccctg caagaacccc tgcgcggacc gctcattgcg tgaaagcccg
     1981 aagcccgaaa ttcgaacttc gaaattcgaa gcaatgactc gaacttcgat tttcgaactt
     2041 cgaatctcaa acttccctcc catgctcccc gtcaccgaac aactcgaaat cctcaagcgc
     2101 ggcaccgtca ccatccacag tgagaaggag ctggcggaaa agctcgcgcg cggcaagccc
     2161 ctgcgtgtga agctcggcgt ggaccccacc tcgccggaca tccatctcgg ccatgccgtg
     2221 gctttgcgga agctgcgcca gttccaggag ttggggcacc agatcgtgct catcatcggc
     2281 gacttcaccg ccatgatcgg cgaccccagc ggccgcagca ccacgcgtcc gccgctgact
     2341 tacgaggacg tggtcgccaa tgcgaagacc tacaccgacc aggccttcct cgtgctcgac
     2401 aaggaaaaga ccgaggttgt tttcaacggc cagtggttca aagagatgcc cttcatggag
     2461 gtcatcaagc tcaacgcccg cgtcaccctc cagcagatgc tccagcggga ggatttcaaa
     2521 aaccgcgtcg ccaacggcac cgaggttcgc ctgcatgaaa tccagtatcc catcatgcag
     2581 ggctgggaca gcgtggtggt gcgcgcggat gtcgagatcg gcggcagcga ccagcttttc
     2641 aacattctgg tgggccgcga cctgcaaaag gaggagggcc aggagccgca ggtgtgcatg
     2701 accctgccgc tgctggaggg gctggacggc gtgaagaaaa tgtccaagag cctcggcaac
     2761 tatgtcggcc tcaccgacgc gcccaaggac atgttcggca agctcatgag catcccggac
     2821 gagttgatgg cgaagtatta cctgctcgtc ctcggcgaga cgatggacgc cgccatgcac
     2881 cccatggagg cgaaaaagca gctcgccgac aaatgcgtgg ccgtctatca cggcggggaa
     2941 gccgcccgcg agtgccgcga ggactgggac atccgcttca gcaagcgcga cctctcctcc
     3001 gccgagctgc cgtctttcac tcccggtgag cggcgggatg tgctcggcgt ggtccagcac
     3061 gctttccaga gtgtctttgg ccaggccgtt tccggggggg atgtgcgccg cctcatccag
     3121 ggtggcagca tccagctcaa cggcgacaag ctcaccgacc ccaaggccga gccggcctgg
     3181 gaaagcggcg cggtcctcaa gctggacaaa aaacgcagcg tgcggctggc ctgagctcat
     3241 gtggtggagt gagggcgcga atgcgagccg gtgatccccg caccgactca acgcgacaga
     3301 ctcgccgtgc gcaccagcag ttgatccagg gtgggcaggt cgtgagccca gaggatgccg
     3361 gggtcttgaa tctgccgtga gtaggattcg gcggcgcgtc cgccgacgat gagagcgatg
     3421 ctctccggca gcaggccgcg aattttgcgc agcttctcct gaatcaccgg acagtgcatg
     3481 ggatagacaa cgctcagagc cacggcccgc gcgcagcggt tgcgcgcgca ggcggcgatc
     3541 tcctctgtgg gcaggttggg gccgaggtag gtcacgcgcc agccgaggtc gcgcgcggat
     3601 acggcagcca gcagggcgcc cagctcatgc acctcccccg cgggcgttgc gacgacgatc
     3661 tccggcgctg aggcggccac ctggctgccg ggcacgggtg tcatcaaaaa ctcccgcacc
     3721 acggcggtgg cgatgtgctc gtggcccggt ctcaagctgc ccaactgcca ggcgtctcct
     3781 atctcatgaa tgagcgggca gatgatttgg tgcacgcagc agcgctggcc gaactggatg
     3841 cgcgcgcgct gcagcaggcg gtgcaggcgc tcgctgtcat aggcgcgcgc ggcatccaga
     3901 caggcgcgca catattccgc cggcgactca agggcaggat ccacggtgaa gctggccgcc
     3961 gccgccactg gccggcttct ggatttttcc aactggcttt ccagctccgc cgtgctgagc
     4021 cgggcaatgc cgccgatgcg gtgcccgcac tcggtgagct ggcgcagcag cgagagccgg
     4081 tggatgtcct cgtcgcaata aagccggcgg ttcgtgcagg tgcgctccgg acacagcgcg
     4141 ttgtaacgcc gctcccagat gcggatgacg tgcggtgtga ctccgctgcg cagactggcg
     4201 acttggatgg tggatgacat gtgcggctga ctgtgggcag cgcggcgagg gaaggtcttg
     4261 ccagagggcg ccgccgcgct ttacggtcaa ttctacgccg tgctttccgc gccgggcgca
     4321 ctcatctcaa cacctttttc catgctcaac cacatctggg ccctgctgct gctcaccggt
     4381 cttgtcgtcg ccggatggtt cggacgcttc tccggcgagg ccggcgtcac cgcctccgcg
     4441 ctcgccatca cccgcaccgc cgtgatggac atcgccctgc cgctggccgg catgatgatg
     4501 ttctggctgg gggtgatgcg gctgatggaa aaggccggcc tgctggagct ggccgcgcgc
     4561 gccgtggctc cgatgatgcg gcggcttttt cccgacgtgc cagccgggca tccggccatg
     4621 agctccatga tcatgaacct ggccgccaac atgctcggcc tgggcaacag cgccacacct
     4681 ctcggcctca aggcgatgac gcacctgcag gagctcaacc cgcgcaagca gactgccagc
     4741 aacgccatgg tgacctttct ggccatgaac acggcggcct tcaccctgct gccctccagc
     4801 gtcatcaact acctcaccgc cgccggggtc agggaggcgc accaggtcat cgtgcccacc
     4861 atccttgcca ccgcctgcac cacgctcacc gccatcctca tggcgcgcgc gctgcagggg
     4921 cttcccgtgt tccgcgccgc tcccgacgag ggtgacgccg ccgcggaggc agcggaggga
     4981 agcgccgccg cgccccagcc cgcggggcgc aaaacccgct ggctcgtcgt gacgctggcc
     5041 gtgcttttcg gcggcggcct gctgctggag ttcagcccgc cgcaagcgcg ggaaagagtg
     5101 ctggaaacca ccggcctgag ccgggtcatg gagcgcgcgg gacagaaagc cgccgcccgc
     5161 caggcgccag cgccggtgga aaaggcggag gagccgcccg cgtggcggcg gctgatgaac
     5221 gccgggtccg gcgcggccat tccgctgctg ctggtggtga cgctcggcgt ggcgctcaca
     5281 cgcggcgtga aggtttacga ggagttcgtc gagggcgcca aggagggctt cgcgatggcg
     5341 acgcgcatca tgccctttct ggtggcgatg cttgtggcgc tggccgtttt ccgcagctcg
     5401 ggcgccctgc tgattctgga gatgatcctg gcgccggtcc tcgcattcgc cggcttcccc
     5461 atggatctgc tgcccctcgc actgatgcgg ccgctgagcg gcagcggatc ggtcggcgtg
     5521 ctcaatgaaa tcctcaccct ccctgaggcg ggcgagaccc tcaagttcac cgccgccatc
     5581 atgttcggct ccacggaaac caccttctat gtgctcgccg tgtatttcgg ctccgtcggc
     5641 atccgcaaaa tccgccacgc catcctcgcc ggcctcgccg ccgatgccgt cggcatcatc
     5701 atgtccgtgg tcatcggcag gctgatgttt ggatgagccg gcagcagcgc cggggaaggc
     5761 tggaggcggg gagtcacttc atgcccagca cgcgcaggag gaaggcgtat gtgtcggcgg
     5821 tctcctcgat gacttttttc agcgccgtgc ccgcgccgtg gccggcgctc gtctcaatgc
     5881 ggatgagcac gggcgggccg tctttggcct ggcactcctg gaggcgcgcg gcgaatttga
     5941 agctgtgcgc gggcaccacg cggtcgtcgt ggtccgccgt cttcacgagt gtggccgggt
     6001 agcgtgtgcc gggcttgaga ttgtgcagcg gtgagtaggc gcgcagggct ttgaactcgg
     6061 cctcgttctc gctgctgccg tagtcgcttt tccatgccca gccgatggtg aagaggtgaa
     6121 agcggagcat gtccatgacc cccaccgagg gcagggcggc tccgaacagc tccgggcgct
     6181 gcgtcatgca cgcgccgacg agaaggccgc cgttgctgcc gccctggatg gcgagctttg
     6241 cgggcgaggt gaacttttcc ttttgcagcc attcggcgca ggcgatgaag tcgtcgaaga
     6301 cgttctgctt gcgcagcctg gtgccggact ggtgccactc cgcgccgtat tcgccgccgc
     6361 cgcgcagatt tgcctgcgcg taaatgccgc ccatctccag ccagaccacg cggctgacgg
     6421 agaaggcggg tgtgaggctg atgttgaagc cgccgtagcc gtagagcagg caggggtttt
     6481 ggccgtcgag cttgacgccc tttttgtgga cgatgaacat cggcacgcgc gttccgtcct
     6541 tgctggcggc aaagatctgc ctcgtctcat aaagggcggg atcgaagtcc accctggggc
     6601 gccggtgcag gaagctgcgg ccggtttcga catcatagcg gtagatggcg ccgggttcgg
     6661 tgaagctggt gaaggcgtaa aagatctgct tttgatccat gcgcccgccg aacccgccca
     6721 cagtgccgat gccgggcagt tcgatggagc ggatgagcgc gccgtccagg tcgtgcgcgc
     6781 gcatttcgga gcgggcgtct ttgaggtatt cgcagaggag ctggccgccg acgatggacg
     6841 cgccctccag cttgtcctcc tgctgcggga cgatctcgcg ccagtggacg cgctgcgggc
     6901 gggaggtgtc aatggcgatg acgcggtaac gaggcgcgtc gagatcggtg cggaagtaga
     6961 aaagcgcgcc ggcgttgtcg agaaaactgt agtcggcgtc gaagtcggcg agcagctcga
     7021 cgacaggcgc gtccttgtcc ttcaagctcc ggtagaagac gcggtttttc gggtcggtgc
     7081 cgtgcgtgac ggtgagaatg aggtaggcgc cgtcatcggt gacgcgggcg tgcaggcccc
     7141 agtcaggctg gtcgggccgc tcatagacaa ggagatcggc ggattgcggc gtgccgagtt
     7201 tgtggaaata gaccttctgg tttttgttcg cctcggtgag cgcggcgcct tcttttggcc
     7261 gcggatagcg gctgtagtaa aagccggagc cgtcgcgcgc ccaggacgcg ccgctgaatt
     7321 tgatccactc gagcacatca ggcccgtccc tgccggtggc gatgtctttg acgcggaact
     7381 cctgccagtc gctgccggct tttgacaggc cgtaaacgag gaagcgcgcg tcacggctgg
     7441 gcgcggtgac ggtgagggag gtggtgccgt cctcggagag cgtgttgggg tcgagcagca
     7501 ctcttggttc ggcgtccggg gagtcggcgg tgagcagcac gccctggttt tgcaggccgg
     7561 agttgcgcgt gaggaaccac ctgccgccct cctcccaggg cacgccgaac cgctcgtaat
     7621 tccagagctt ctccagccgc gcgcggatgt ccccgcgccg ggggatggac ttcaggaaat
     7681 caaaggtgac ggcgttttgc gccttcaccc aggccttggt ctcctcgctg ttgtcatcct
     7741 ccagccagcg gtagggatcg gggattttga cgccatggta ttgatccgtg acatcctgcc
     7801 ggagggtccc ggggtaggcg atggtctggg cgtgtgagct gagagcggac atgaggcaga
     7861 gaatgagcag ggcgcgcatg gccgcgcatc atgcagggcg cggaggcggg cgcaacatgc
     7921 cgccgcggcg cgaagcaaag acggcggggc cgcggcggaa aaataatcca gagcagaatt
     7981 gacactctcg cggcgcggcg gctatagttt aactctatgt taaggcaaat catcggacaa
     8041 cccactgaag gagccaagga acccgcgcgc acgcctgcgg caccagccgc cgagcgcgct
     8101 ccgatcctct cctctccctc cgcccccgcg tcacaacccc accgctctgt ctcctcatcc
     8161 cccaatatga cctccagcaa aaacatcctc gccaacgacg tcgagatcaa aggctcgatc
     8221 aaattctccc acgacctcat cattgacggc aagatcgagg gcgaagtcca ctccgacggc
     8281 agcctcactg tcggcgagaa cgcgctcatc aaaggggaga tcaaaacccg ctccgtcatc
     8341 ctcttcggca aggtcgaggg gaacatctcc gtctccgagc gctgcgaact caagagcaat
     8401 gccatccttg tcggcgacat cgaggcaggc acgctctcca ttgaggaagg ggccactttc
     8461 atgggcgctt ccaaagtcgg acgcaagccc gagccaaagc cggctcccgc aggcgtgaac
     8521 cgccccccgc aaccctgatt gagcaccaag ccctgtgttc acgggtcttt tcggcaggaa
     8581 aagcgaccgc ccccctccac ccaagaatca aatcgaggtg gagtgcccgg cgtgcggcgc
     8641 ttatcaatac gagccgaaaa tggtggtctc caccttctgc cgcaagtgcg gcgtccatct
     8701 cactgtcacc aaacgcggtg tgaccgcctc caacgccacc cgctccggag tgggaatgcc
     8761 cgacccctgg gcgaaggagg ctgctcacgc tccgaaaccg ccggaaaaac ccgcttcatc
     8821 acccgccact gaggccgccc tcgtcatgga cgagaccgcc ggcgagttgc gccagcccgt
     8881 cagcgaggac gaggccgccg agggcggttt cggcgtgttc ttgaaacagc agcttgccgg
     8941 agcgcagccc gcgaccaccg ggcacaacgg cagccatgct cccgcgcagg acgaacccgc
     9001 cgcggaagct gagctggtcc ccgccgatga agctgaagag ggaagcggcg cggacggcgc
     9061 tgcggtcaaa cgcccgcagt caaactcccc tccgcccact cccgctccgc ccccgatgaa
     9121 cgagggcact ctgcaaaaaa tgcgggacgc gggaatctac cggaaccatt atttcaagga
     9181 cgcgcactgc tttgaatgtg gccacaagtt caaggtcaac cgctccctgc gcgccgtcac
     9241 ctgcgccaac tgcggcgccg cgatccccat ggaggatgtg gagatcaaca tgctcaccga
     9301 ccgcccgatc aagacccgcg gcgatgtcat catccgcaag cgcggccatg tccgcgcgga
     9361 atacatccag tgccgcgagc tgcgctgctt cggcactttt gaatccaacg tccaggccga
     9421 gggcgatgtc gtgttccgcg ccaatggcag gatcaccggc aggatcgaat gccgccggct
     9481 catcatcgaa aaaggctcgg atgtctccct gctcaacgaa gcccgcgcag cggagatcga
     9541 ggtccatgcc aaggtcgccg gcagcctggt ctcgacaggc cgcctgctca tcggcgccaa
     9601 cggctctgtg gatggcgatg tcaccgcgcg ctccgtctct attgagccgg gcggcgaatt
     9661 gaacggcgcc atgaacattc tcagcggcgt catcaaaaac gcggactgag ttttgccgca
     9721 gcggcgtttc cccgcgcaat gaacgcccgg actgccagcg gtctccaagc aggaatgatc
     9781 cgcttcctca cgctcctgct cctgctcgca tcctgccacg ccagccagga ggaacaggcc
     9841 gccgcctccc gcgcccgcct gcccgatgac ggctcctttg ccagccggct gtgcctggcc
     9901 gcgctagagc gcacgcgcca tcaagtccgc tacgaccccg cctacgttcg catcccttat
     9961 cctggcggtg acgtgcctgc cgacacaggc gtttgcaccg atgtcgttgt ccgaagttat
    10021 cgcgctctcg gcattgatct gcaaaaggag gtgcatgagg acatgcgccg cgccttttcc
    10081 gcctacccga agaactgggg actcacagct cccgacacca acatagacca ccgccgcgtg
    10141 ccgaatctcc agaccttcct caagcgccgg ggcgcggcgc tgcctgtcac gcaaaacgcc
    10201 gccgactacc tgcccggcga cctcgtcacc tgcacggtgg ccggacgcct gccccacatc
    10261 gccatcgtcg tgcccgcgcc cgacgacggc cccgaaccgt ggatcgtcca caacatcggc
    10321 cagggcccgc gccttgagaa acgcctcttc gagttcccca tcaccggtca tttccgctgg
    10381 cgggggaagt gaggaaaagt ttgtcctccc cttcggatga attatcccat gcccattgaa
    10441 tccgtgtgat ccgtggattt gaccctgtcc cggatttatg atccatgcat ccagtcactg
    10501 cgtcagcgca tcaccgcgcc ttccgggcag acgagggcga ggtaggcggc gacgcgggcg
    10561 cgggcggcgg tttcggcggc gggtatggca ttgactgcct gcaagatgag agcgcgagtg
    10621 ccgcgcgtca tttctccggc gcagaagagg gtgttgaggt ggtccacgag gcgctcgggc
    10681 tgggcggcga gggagcgctc gcggctgagg tctatgggga agcggtattc ctcccagtaa
    10741 ctgaagccct ccatcaccat ctgccagagg cggttgggaa aggagatgga ggagtagctg
    10801 tcggtgatct ggaaaacggg gccggctttg ccctgctggg tgagcagtcc gggggcgcgg
    10861 tagtcggggc ggtagaaatt gaagacgctg ggggagtagc cgggctcctg gcggctgtca
    10921 ttgtagaagt cgttccagtc ccaccagagg aggtccggca cctgcttcat gccaaaggca
    10981 cggccaaggg cgatggcgcg gatgacgggt tccttgagcc ggccatagtc ggcgggacgg
    11041 ctggggccgc gcgcttcggg gtcgagcagg atggcccgga cgacggagaa tagattgccg
    11101 cgctggccgt agtcgtcact ggtgaagaca gtggcgacgc gtgcgacata ggcgggagtg
    11161 ggattgtcag tgacgaggaa ctggatgagc tggcggctga caaatggggc ggtgttcggg
    11221 tgctcgaaga ggtggcggat ggcgtcctgg atgtcgcgca tggcctcggc gtgagtgggg
    11281 gtgcgggcgg ggatgacgaa gccgtcgagc agggtctttt ttccgaagtc gtgccggtcg
    11341 gcatgcatgg tcatgggtgt ggcgtaatcg gtctccgtcc agccgccgcc gccccagtaa
    11401 tggccgccga accagagacc ggtcagcaca cgggcgagct gggtgatctc ggcattggag
    11461 taggtgggga tgggctggcc ctggctgtcg agctggcggg tgccgtcagg attcagccgc
    11521 cacaggccga tggtgaagag ctgcatgacc tcgcgggcgt agttttcgtc cggatactgg
    11581 ttgatctccg ggcgggcttt ttgattgccg acatggctga ggtaacggcc catgacgggg
    11641 tggagtgtga cctccatgag gatgtcatga taacttccga aggcgtggcg gacgaagatg
    11701 tcgtaatagt cggccatgcc gaggcagcgg ttctccagat tggcatcccg gcgggaggtg
    11761 acgaggattt gggaaagggc aaaggccacg cgctggcgga gctggtccgg cccctggatg
    11821 gcggcgcggg cgaaggcggt cttcatgttg ccgccgaaga ggaagggatc gcggtcctcc
    11881 ccggcgacga cctcgcttcg gacgtagttt gggttggcgc gctgagtcat catgtcctgc
    11941 cagatggagc ggatgtagtc ggagtggagg gtgggcgatg cggcgcgctg ggcgttgatc
    12001 cagccttcga agccgagcga ctgcacctgc tcgatgtcct ccagcgtcgg gccgaaactg
    12061 gcctgggtga ggaagcgcgc ggcctggctg cgggagatgc cgccgcccgc gccgccgggg
    12121 aagccgcctg cgggcgaggc gccgtggagt ttttccaaaa gggtggcgtg gtcgccgctg
    12181 agggcggtgt ccggctggcc gtcgtcattg ctgtccacag gtgccggggc gcgggcgctg
    12241 tcagcggcga gacggtcgga gccgaggacg ctgacctcgg cccagtcgct gacgccgtcg
    12301 ccatcggtgt cgagatcggc gacggtggct ttgaaaaagc gcggctgtgc gccggtgagc
    12361 gcgcccgcca ccgtctggcg gtattcattg ccaaaggcga gcggcacgcc gggcgcgggt
    12421 gtccaggtgg cgggggagag gctgtcattt tgccagaggc ggtggtgctt gagagcgagc
    12481 agtggccagg cgacgacgag gtccgacccg gcgcgggtgg tgtccaccca aaggcgggag
    12541 taaggatcaa aaggatcagt gccggcggcg gcctcgtcgg cattgctcat gccgtcgcca
    12601 tcgtcatcgt cgagggcggc gagatcgaag cggctaagcc agagttccca ggcgtccggg
    12661 atgccgttgc cattgaggtc ggccccgctg acgccggcgg gcgggccgtg ataccagggt
    12721 ggcaggctgg agccgagccg gggatcactg ccggctgcga cctcaactcc gtcaggcgcg
    12781 ccgtcgttgt cactgtcgga gttgtgagga ttggttccag caaggaattc ctggaggttg
    12841 gtgaggccgt cgttgtcggc gtcgagactg gcgtcggcgg ggttgttttt gtcaaaaccg
    12901 tgcaggttct cccaggcgtc ggtcatgccg tcgttgtcgc tgtccttcca tgcggcgaag
    12961 gcggttgtgg tgtcgagcct ggcgctgtgg atgaagacac ggacgccgtc attttgataa
    13021 aggctgtata tccctggagc gccgctttga tcctgccagg tggcactgtt gttgtggacg
    13081 ctcaaggcga gggcgcaact ggcaaaggtg gtgctggcga cggtggtgtt cgtgtcctgg
    13141 ttgaagatgg aaaaggtgaa ggtccagttc gtgcgcgagc cggggctgct gccctcgatg
    13201 cggaagcgca ggcggtcgct catgtccacg gagccgtgac cgctgaagcc gagcgcggga
    13261 gcgaggtcag tcggcggctc attccagtca ctttcccagt tgtcccaact gttgttgtcc
    13321 gggtggctga aggcggactc gtagttcgag taaaagtagc gggtgatgcg gccggagacg
    13381 acgcgcagcg ccacccagaa ggcgtcttga ttcgggaagg cggagttccg cagggtgagg
    13441 cgcagcagag tgtcatcccc ccattgcagg ccgctgtgat ggccgcgggt gtggtcgcgt
    13501 atgatctgca cattttccac ctgccagagg aaggtgcgcg gcgaggtggt gacttgcgga
    13561 agaaaagcac cggcgctttc ggcaaagagc gggtcgcgac cgaggcggat ctcttccgcg
    13621 tcgtccatgc cgtcgctgtc ggtgtcggcg agcaatgggt tcgtggggga gggcagtcgc
    13681 gccaattcct caccatcact gagaccatcg ccatcggtgt cggcgatgag cgggttggag
    13741 ccggtgttca aatggctgaa aaaaacgccg gtgtttcttt cgacattgtc ggtgagaccg
    13801 tcgccgtcgg tgtcggggtt gtgcggatcg gtgccgcggg cgtattcgcc ggagttggaa
    13861 aggccgtcgc cgtcggggtc gagggcggcg tcattgaggt cgggccgcag gcggtgggtg
    13921 agttcccagg agtcggtcat gccgtcgccg tcggagtcgg tggcgctgtc cacgatctgg
    13981 atgccgtgga tgccgagttc gtgattgtcg tggggaaaga gcttcacgct ggcggtggcg
    14041 ccggtgacat tgcgccagcg gatggcattg ccgcgccagg gcttggcggg agtggagccg
    14101 tgcggctcgg tgaagcgggt ctccggcgga cggctgccag tgatgaacca gcggtcggtg
    14161 gcgggcaggt cattgaggcg ggcataaccc accgcgccgt cataactgcc gcccgcgtaa
    14221 agaatgacat cgtagccggc gaaagggatg ccgccgatgg tgacggaggc gggcgtggag
    14281 tcactcacga cgaggtaggc gtccatgatc atccggtcag gcgcgccggt gttgccattg
    14341 atccacacat ggttcgggga ggtccaggcc agggtcatgg tggtgggcgc gccggcgctg
    14401 ttgaccagca caccctgctg cggggactca atgtgcatgg tgttgcctga ggtcgtgttc
    14461 cagctcgtga gcggcatcgt gttgttccaa tgaagctggg gcgagagacc ggcgggggcc
    14521 agccagggca gggcgttgtg gggattgaca tcggagacga aattcaccgc cacagcgtcg
    14581 gggaagacgg gcggcgtgct ggcggcgctc atggcatcgt gccccgtgcg cagctcccag
    14641 gcatcaggcg cgccatcggc atcggagtcg gcaaggagtg ggctggtgtg atgcgtcagc
    14701 cattcatcgc cgtcggagag cgtgtcgcca tcggtgtcgg cgaggagggg attcgtgccg
    14761 agttcggaaa tgccggcata gacgccggtg ttcgtctcca ccgcgtcgga aaggccgtcg
    14821 ccgtccgtgt cggcgaggcg ggggttggtt ccgcgcgcga actcctggag atgggtgaga
    14881 tcgtcgccat cgaggtcgag cgcggcatcg gcggggttgg tatcgctcag gccgtgggtc
    14941 tcctcccacc agcgcggcag tccgtcgctg tcggcatcga gggtggccgt gggatcaatc
    15001 tcgaaagcca gcgcgtcaat gtgaacccag ccatcggcgc tgccgccgga gcggatgacc
    15061 tcgatcgtat tggcacccgt gtttggcgtg gcatcagcct cgacgacaag cgctccgtat
    15121 gtggaggcgg tccgcgtgcc gatcaccgcg ccgttgagct tcaccgtgaa ctcgtgcgtg
    15181 ccatatccac gatccgccgg cgtgcctccc cagcaatgat ggaggatgaa ccgcacccgc
    15241 gcggcccccg cggcttgccc ggcgctgagc atgaaatggt agcgctggct ggcggaattg
    15301 tcgccgctgg ccagggcatt gtccgcctga ttcaccggct cggcagcggc cacgactccc
    15361 acgggatcgg agtagtcccc ggcaaagtaa tagtcgtcat ccagaaccga ggcgctgccc
    15421 ggagatggaa aaatgcccca gactgagcca aacccgttcg gagtcccgtc atgaaccccc
    15481 agtgtccaca ccgtggcaaa ctccgcgcgc aaggccgccg gcagcagcag gcagaggcat
    15541 gacagtaaac gcacggcaaa aaacatgact gccggagtgt aaatccccgg cgccagcgcg
    15601 gcgacttttt ttcagcggca tcctgcaggc cgccgcaaaa gtcgaaattc gagtttcgaa
    15661 gttcccccat cagcgcccat tgtgatcgcg gagcgtctcc agcacctctc agactctgcc
    15721 ccatgccagc acccgccagc cccagcttcc cagatttccg cgccgacttc cccatcctcg
    15781 gccagcaggt ccacgggcac ccgctcatct attttgacaa cgccgccacc agccagaagc
    15841 cgcgccaggt catcgaggcc ctcacccgct actacgagcg ggacaacgcc aacgtccacc
    15901 gcggcctgca cgagttgagc atgagggcca ccgacgcctt cgaggcggcg cgcgcgcgcg
    15961 cggcccgttt catcggcgcg gcgagccccg acgagatcat cttcacgcgc ggcaccaccg
    16021 agggtgtgaa cctcgccgcc caagcctggt gcgcgcgcca cttgcagccg ggggatgtga
    16081 tcctcaccac cggcatggag caccacagca acctcgtgcc ctggcagctc gccgcccagt
    16141 gccaccaggc cagcctgcgc cacatccccg tgcttgatga cggcacgctc gacatggacg
    16201 cgctcgactc gctgctcgac ggcccggtca ggctcctcgc cttcgtccat gtctcgaact
    16261 ctctcggcac catcaacccc gcgcgcgaga tctgcgcccg cgcccgcgcg ctcggcatca
    16321 ccacttttct ggacggcgct caggccgcag ggcacatgcc cgtggatgtg cgcgggatcg
    16381 gctgcgattt ctacgccttc tccgggcaca aaatgtgcgc ccccaccggc atcggcgtcc
    16441 tgtatgcccg gcgggaggtc ttggaggaaa tgccaccctg gcagggcggc ggcgagatga
    16501 tcctcagcgt ggccctcgac agcagcacct acaaagcgcc gcccacgcgc ttcgaggccg
    16561 gcacccccga catcgccgga gccatcggcc tggcggcggc gatggactac ctcgatggca
    16621 tcgggctgga ggccatccag gcgcatggcg aggcgctcac ccgccgcgct tgggacggct
    16681 tgagcgcgct gcccggcatc cgcct
//
LOCUS       DAOMYJ010000108        16673 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Prosthecobacter sp. isolate AMR_MDS_5629
            NODE_1202_length_16673_cov_25.452341, whole genome shotgun
            sequence.
ACCESSION   DAOMYJ010000108 DAOMYJ010000000
VERSION     DAOMYJ010000108.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867850
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prosthecobacter sp. (wastewater metagenome)
  ORGANISM  Prosthecobacter sp.
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Verrucomicrobiaceae; Prosthecobacter.
REFERENCE   1  (bases 1 to 16673)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16673)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.30
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:34:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,742
            CDSs (total)                      :: 4,684
            Genes (coding)                    :: 4,676
            CDSs (with protein)               :: 4,676
            Genes (RNA)                       :: 58
            tRNAs                             :: 55
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16673
                     /organism="Prosthecobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1202_length_16673_cov_25.452341"
                     /isolate="AMR_MDS_5629"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1965333"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..350)
                     /locus_tag="PLP58_12290"
     CDS             complement(<1..350)
                     /locus_tag="PLP58_12290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ09346.1"
                     /translation="MKHIPLLTLALASALPCMTGAQENGKPAGISAAEFHSRLTRDAA
                     PLPDGGQLKMSYADVVDKILPSVVTIFSKGGKSGAPDVEDIPPNLRPFFYHFFGVPPP
                     GEDEEEPAPRGRRGA"
     gene            complement(499..1464)
                     /locus_tag="PLP58_12295"
     CDS             complement(499..1464)
                     /locus_tag="PLP58_12295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009965096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HRJ09347.1"
                     /translation="MIRTLSLLVAALFAVSASQAQNIRAGIIGLDTSHVLAFTKTLNA
                     EPQKPEVMGVRMVAAYAQGSKDIESSVKRVPEYTEKVREMGVQIVGSIDELLDLVDVV
                     FLETNDGRPHLEQLRPCLAAGKPVFIDKPVAGSLRDAITIFKEAKAAAVPVFSSSSLR
                     FGKDSQAVRAGSIGRVKEARTHSPASLEPSHPDLFWYGIHGVESLFTVMGKGCVSVKR
                     GATGEGKIQVTGTWEGGRNGTFTEGKGYGGTAIGEKGEAPVGSYDGYDPLLFSAVHFF
                     RTGVAPVTPEETLEIYAFMEAADESKRRGGAEVTLKEVMEKASAP"
     gene            1687..3054
                     /locus_tag="PLP58_12300"
     CDS             1687..3054
                     /locus_tag="PLP58_12300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009963229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HRJ09348.1"
                     /translation="MKKIPLTSRRKFLAQSAAALGFPSIIPSSVLGQNAPSNQITMAV
                     FGWGMMGPGNTNKFMAEADCRVLAACDIDKRNLEKAVGTINGYYKNTDCKGYHDYREV
                     MARKDIDTVMLALPDNWHALTAIEAAKNGKDIYGEKPLARTIAEQQAIVRAVKRYGRI
                     WQTGSWQRSEDNFRIGAEIVRNGLIGRLTHVEVGLPSGHNDFAKTGDKRSVTPPPPEL
                     DYEMWIGPSAMQDYIECRVHKNWRWDYNIGGGQLLDWIGHHCDIAHWGMDMDGSGPLE
                     VTPVQVDMPPRTDIWNTATKYRAEARYEGGILMTLAGGHEDIKMGTKWIGADGWVYVN
                     RGGAYDSSNPALKQIIKKREGDKVVESAKAPKLGDDVIKTRLYETPGHHRNFLDCVKS
                     RKPTVTPAETAHRSAVPGHLALITFLTGRALKWDPAKEEILGGDTEAAKLLGREYRAP
                     WKLEA"
     gene            complement(3891..4322)
                     /locus_tag="PLP58_12305"
     CDS             complement(3891..4322)
                     /locus_tag="PLP58_12305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ09349.1"
                     /translation="MPFDPSSNPAHSRRCALTAFIASMMIVGCAGAGFRYQDSFADLN
                     RKELTTDSIVQTLGEPARISRATGETKEGPPADTAYRYYFATRDGYPGAKIFYFYRGR
                     WTGENVLGGERRNEIFRLLDPSKKTDKKLIDEWEAEHPHVW"
     gene            complement(4425..4775)
                     /locus_tag="PLP58_12310"
     CDS             complement(4425..4775)
                     /locus_tag="PLP58_12310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HRJ09350.1"
                     /translation="MVEPLYEREADAVPGPFYVVKDQCIICDFPPELAPKCLKMKDAP
                     CNADKYCHVYKQPQTQEELDTVIEAMAGSCVQAIRYCGSDLALLNRLVSLGMAHLCDA
                     TEEARQNKRMESNG"
     gene            5278..7716
                     /locus_tag="PLP58_12315"
     CDS             5278..7716
                     /locus_tag="PLP58_12315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ09351.1"
                     /translation="MTISPDQYEKLGTFYLGRGYDLGAKQLQDDLVLYDSKDLVTHGV
                     VLGMTGSGKTGLCLALLEEAAMDGIPLIAVDPKGDIGNALLTFPNLSAEDFQPWVNED
                     DARRKGVSVEQLADSQARMWSKGLGEWGQDAARIRRLRETVDMAIYTPGSSAGLQVSI
                     LSSLECPGPELMEDTEILAERIESTVSSLLGLMGVDADPVQSHEHILLSNIIGHCWRA
                     GQSVSLAHLVRHIQQPPMRAIGVVDVETFLPEAKRSALAMKLNNLIASPGFASWLEGE
                     PLDIQRMFYTAEGRPRVTIFNIAHLGDNERMFFVSLLMNQLLGWMRRQQGTTSLRALF
                     YMDEIFGYLPPTAMPPSKKPMMILLKQARAFGLGLLLATQNPVDLDYKALSNIGTWWI
                     GRLQTERDKARLLDGLAGAAGEKFDRKAMETAISGLGNRVFLMNNVHEDGPAVFHVRW
                     VMSYLCGPLARDQIKRLMDPLRPAKAAAPAAEDDGFLPPGATAAPAAERKTHKPKLPE
                     TAAEYFLPTDTPADSLCYVPAILRSATVQFEDTRRKISGSSRITLVNPIDTAGQRVLW
                     DKFVELPRDSEPARWHTAQVEGAEFADLPGAALKSSTYTSIKKDYADWVYANHSIEVG
                     FSPLLNTGSNPGEKLDEFRARVAHTARELRDQAIEELREKTAKSLKTLQDREMRAQIK
                     VDAQKSQATSAKLSTAVSLGSSLLGAFFGRKSGIGSLMKTTTVTGAARVMRESQDVAN
                     AQTELARVQEDIALLEKQTGEETASIRGQYDPATLALETLKLTPVKSRIQVDAAGIVW
                     LPHERAGDSLRPAF"
     gene            7851..15017
                     /locus_tag="PLP58_12320"
     CDS             7851..15017
                     /locus_tag="PLP58_12320"
                     /inference="COORDINATES: protein motif:HMM:NF020390.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1800 family protein"
                     /protein_id="HRJ09352.1"
                     /translation="MPPPNPPPAMSSRLTQPLVSSWKTVAAALCLLPVLHLQAITDFN
                     KWPAGQEPNGQTNGPDGLDDVWQSIFNAWNIDPQGDEDGDGCSNLIESIAGTNPFLAG
                     DCLRMGDTIIAASTVVFKVNAKAGKKYTVRSDDNPNGAFTAVEPLLTPVAAPHFLAAS
                     DNPNLTLSIAKNGGTEKFYKVEVSDHDTTGGGVSDWAKLKLGMNPALADSNDDGISDA
                     EEIIEELQIPDEVTIIASKSFASEDGPDSGTFTVSRSRSLLGAAANYSVSGTAVAGVD
                     YNRTPVGTVTFAPGVKTATIHVNVLPDSGVEGSESVTATLTTTTGEAYPPPSIGFPDS
                     ATVIIQNNTAPTGTGLLGRYYDHASTIYAHGANFGFTANYAYTRAGTSPDFTGTTVVT
                     PTAGSPSGNLANLQVGHVVRLTFLGGNLNTAAYNHLDYTVTAKTATNFTVQMPPGASL
                     PANSSSTCNYSIQSILHPPVIERVDPTVAFEWMGGTPNGNSISPGNLADNWSSVWEGY
                     LHPTTTGNHRLQLDADDKARVLLDLNGNGVFDLPGEQIVEHGWDTAATASPEDGVADD
                     ETIGTFKISANHALTIPASAAQRYKMRVEMVETTGEARCRLQWSINNGTFANIPSANM
                     FTHTQGMTSNYDFNRLTTGSPMTGTITVRLNAHGFSVGSPVELAFSSGVLFTPANGNF
                     HGSYTVATVNSANQFVVNISAPSLPASSNNSGGGFILNRPASTTAGLLNWVWQNSSFS
                     GAPGRVGTNTNGTNDSNNGLYGTGTPDDALINPDSFSVRWTGQMQPQFTEEYTISVFA
                     DDGSRLLINGVEQDLKMLPSANTGGSTYSYDSATGDAVVTYTNAIIKPGSIVAGEVLR
                     FDPANGVLALGNGSTYTYDSVTGDAVINYSNLTNVNPGGFVVGETIEVDPTNGTATSL
                     ALLPYQITAATATTFTVNFGAGVFESGTGSVNVSDNSDRVVTAATATTFTVNFGAGKH
                     LNGTGTMNFNFVNKPVKPWASNGNERYVRLPLVGGVRYDIQLEYYESGGFCRCWLYWM
                     SPSQPKQVIPTERLYPSSDPVIAPASHVSPTDATALVGGNFSHAVEGSNGASVSLSGA
                     PAWLTFANGVLSGTPPPGSAGIYQILITLTNAAGTSTSVLNLTVEQSGGSVEREVWTG
                     IAGTSVSSIPVGTTPNSTNTLTSLAAQTDFGDNYGARIRGYITAPVTGNYYLWVAASN
                     SAELWISNDDDPINSFKRASVLSGHSGGWSWNAEPSQKSAWLALKQGKRYYFEVLHKA
                     GTGAGDNLAVGWSKPGENESAPAEIVPGHVLTPFVPPAPGSTPGTLYVATMLSQGGAI
                     TNGVGTATLRLSEDETVAHVAFTHNGLTGPVTDWHVHSDPFLTHQSAIIYDGTEPLPG
                     DGPQPDGTHKWNIVTAGTLSAAEIIELLKQGKAYINLHTAEYPNGEIRGNFTLANGSR
                     TFTPPPPPPMWADDSNTNAGAARFLTQASFGPNVADITALKALTATGGSAHYPPSRYE
                     TWIDNQFTQPWSDHLTEVLRTEQSSAQGGAFEEILTFNSWWRRSISGADQLRQRLAFA
                     LSEIHVVSGQGPLDNRAEALSYFYDKLGENAFGNFRAILETTTLTPTMGRYLDMLRND
                     KPDLSTGRIPNENYAREIKQLFSVGLYRMWPDGTLMLNSKDSPIDTYTQSEIVGYSHV
                     FTGWDYGYDGFNRTALNAPANWMRQMRPTPARHFTGPKLLLNNEVLPGLRTLGGQPLD
                     PYANHISAQFNDPAYKALPDYELAVSHDQLFNHPNVGPFICRQLIQRLVTSHPSRDYL
                     YRVVQKFNDNGSGVRGDMKAVIKAILLDYEARSPQMLQIPAYGKQREPVLRVAAAARA
                     FRKAPWSGTYSQSATDPTDPRRIYITTNDPHGFPTGTSNQLLEFTSGNPAPWTGVYSV
                     AQTGANTLRATATGWATGTYSIPANSTTCTITMSNHWIQVGNQFFIDFYTNTSGTANG
                     SAIDQQVYTVATNPNPSSGGNNGSSFTFEIPLAQTSGTLRTGSCMIPRFSPGSYQYSN
                     NNTGVPVDQQGTYNRLVTMDTANNTHHELNVGDQVQLNFYGGNPTPNDAVVTVHSIVD
                     ENTWTFLANNTAMGLGNNAQGFNSVYQFPLKSLPLTRNGNVGARPSTFSMGNTTLELD
                     QAPINSPTVFNFYLPDFKFPGALASQGITTPEFQETAETSVIRQANFIYNGAFGGGLE
                     NISSFNNGSNALLMSFSDWTTGTSAPDLGLGVAVTPTVPWTHNQNIARLIDHLSVLLT
                     ADQLSAQARQIIRDFVSLPISSISTGNPCTITTARSHNLTTGTTVCISGVTNGTYNGS
                     TTLLNSTTTGYSATVTGETTFTIPVNCTSAPNASGLANAHVSTVVYNQGSNSATNPSI
                     ASRKDRIRSIIHLILTSPDFTIQR"
     gene            15024..16511
                     /locus_tag="PLP58_12325"
     CDS             15024..16511
                     /locus_tag="PLP58_12325"
                     /inference="COORDINATES: protein motif:HMM:NF019040.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1501 domain-containing protein"
                     /protein_id="HRJ09353.1"
                     /translation="MNPPKAPNILSTRRRVLRNAAYASMGATAIGSALRDLRLINSAM
                     AAGPITDYKALVCLFLAGGNDSNNWIVPTDTPTYDAYASIRGNLALPSASLLTLRTGP
                     GGSDPAYVDGDGHTLGFHPTCQELRTLFGEGKLAAVMNVGTLVRPTTRAQYLANPTFY
                     RPPQIFSHSDQVTQWQTSIPDQPPITGWGGRVADLLNSVANPSGNISMSVSLNGNNTF
                     EIGNLVSQYHVSQTGAVTLSGALMAGSQPRVKAMKDLIALSHPNLQRQAYSGVLDSAI
                     VTGDLLNSSIAATIEVAQGGTWVWNTAFPATGLGNQLKMIARLIQARGPAAFNMNRQV
                     FFCSVGGYDTHTAQVTDPLNAANQFNPTTGTHTNLLNEVSECMFAFQRAMEQLGVSDK
                     VTTFTASDFSRTFPTNSQGSDHGWGAHHLIMGGAVKGGETYGSLPTFAINGPDDTGTG
                     RWIPTLAVDQYSATLAKWFGLDSNEIAAVFPNLSRFSSSDLGFML"
BASE COUNT         3305 a         5798 c         4689 g         2881 t
ORIGIN      
        1 gcgccgcggc gtccgcgcgg cgcgggctcc tcctcatcct cgccgggagg cggcacgccg
       61 aagaagtgat aaaagaaggg ccgcagattt ggcgggatgt cctccacgtc aggcgcgccg
      121 gatttgccgc ctttggagaa gatggtgacg acgctgggca ggatcttgtc caccacgtcg
      181 gcgtagctca ttttgagctg gccgccgtcg ggcagcgggg cggcgtccct ggtgagacgg
      241 ctgtggaatt cggcggcgga gatgccggcg ggtttgccgt tttcctgggc gcctgtcatg
      301 cagggcagcg cggaggcgag ggcgagtgtg agcaggggga tgtgtttcat ggtcgtgggt
      361 cagggtttcg ttaaaacgct gtggcagtgc ctaaagacgc gccgcgcggg cttccgttga
      421 aaactttcgc gcatacaaaa cggcggagcg gcggcgttac aagcgccttc gcagccccgc
      481 aaaatgatgc cgccctcctc acggagcgga cgctttttcc atgacctcct tgagcgtcac
      541 ttccgcgcct ccgcggcgtt tgctctcgtc ggccgcctcc atgaaggcgt agatttccag
      601 cgtctcctcc ggcgtcaccg gcgccacgcc cgtgcggaag aaatgcaccg ctgagaaaag
      661 cagcggatca tagccgtcgt agctgcccac cggcgcctcg cccttttcgc cgatggccgt
      721 gccgccgtag cccttgccct cggtgaaggt gccattgcgt ccgccctccc aggtgccggt
      781 gacctggatt ttgccctccc cggtcgcgcc gcgtttcacg gacacgcagc ctttgcccat
      841 gacggtgaaa aggctctcca cgccgtggat gccataccag aacaaatccg ggtgcgaggg
      901 ttccagcgag gccgggctgt gcgtgcgcgc ctctttcacc ctgccaatgg accccgcgcg
      961 cacggcctgg ctgtccttgc cgaatcgcag cgatgaggag gaaaaaaccg gcactgcggc
     1021 cgccttcgcc tctttgaaga tcgtgatggc atcccgcagg ctgccggcga cgggcttgtc
     1081 tatgaacacc ggcttgcccg ccgccagaca ggggcggagc tgctccagat gcgggcggcc
     1141 gtcattggtc tccagaaaga ccacgtccac gaggtcgagc agctcgtcaa tgctgcccac
     1201 gatctgcacg cccatctccc tcaccttctc cgtgtattcc ggcacccgtt tgacgctcga
     1261 ctcgatgtcc ttcgacccct gcgcgtacgc ggccaccatg cgcacgccca tcacctccgg
     1321 cttctgcggc tccgcgttca gcgtcttcgt gaaagccagc acatgcgagg tgtccagccc
     1381 gatgattccc gcgcggatgt tttgagcctg ggaagcggac accgcaaaga gcgcggcaac
     1441 gagaagagag agagttcgga tcatggtggc aggcaattac ggccagggca cccccgcctt
     1501 tcattcaccg gcaggggcgc gccatgcagg cacccgggtg ccgggcgctt gtggaaaacc
     1561 cggaacgggt tttggccttt gaaaacaggg aatgcgggcg ctccttttcg ggttgcgcgc
     1621 cgccacgggc ggagctgttt tttgactcga cacaaagcct ccgccccgcc tacaaatcgg
     1681 ctgcgtatga aaaaaatccc cctcaccagc cgccgcaagt tccttgccca gtccgccgcc
     1741 gcgctgggct ttccctccat catcccctcc agcgtgctcg gccagaacgc gccctcgaac
     1801 cagatcacca tggccgtctt tggctggggc atgatggggc cgggaaacac gaacaaattc
     1861 atggccgaag ccgactgccg ggtgctcgcc gcctgcgaca ttgacaagcg caacctggag
     1921 aaggccgtgg gcacgatcaa cggctattac aaaaacaccg actgcaaagg ctaccatgac
     1981 taccgcgagg tgatggcgcg caaggacatt gacaccgtca tgctggccct gccggacaac
     2041 tggcacgcgc tcaccgccat cgaggccgcg aaaaacggca aggacatcta tggcgagaaa
     2101 cccctcgccc gcaccatcgc cgagcagcag gccatcgtcc gcgcggtgaa gcgctatggc
     2161 cgcatctggc agacgggctc ctggcagcgc agcgaggaca atttccgcat cggcgcggag
     2221 atcgtgcgca acggcctcat cggcaggctc acccatgtcg aggtcggcct gccatccgga
     2281 cacaacgact tcgccaaaac cggcgacaaa cgctccgtca caccgccgcc gccggagctg
     2341 gattatgaaa tgtggatcgg cccctccgcc atgcaggact acatcgagtg ccgcgttcac
     2401 aaaaactggc gctgggatta caacatcggc ggcggccagc tcctcgactg gatcggccat
     2461 cattgcgaca tcgcccactg gggcatggac atggacggct ccggcccgct ggaggtcacg
     2521 cccgtgcagg tggacatgcc gccgcgcacc gacatctgga acaccgccac caaataccgc
     2581 gccgaggccc gctatgaggg cggcattctc atgaccctcg ccggcggcca tgaggacatc
     2641 aaaatgggca ccaaatggat cggcgccgac ggctgggttt acgtcaaccg cggaggcgcc
     2701 tacgacagct cgaaccccgc gctcaagcag atcatcaaaa aacgcgaggg cgacaaagtc
     2761 gtcgagtccg ccaaggcgcc caagctcggt gatgatgtca tcaaaacccg cctctacgag
     2821 acaccgggcc accaccgcaa cttcctcgac tgtgtgaaga gccgcaaacc caccgtcacc
     2881 cccgccgaga ccgcgcaccg cagcgccgtg cccgggcacc tggcgctcat caccttcctc
     2941 accggacgcg ccctcaaatg ggaccccgcc aaggaggaaa tcctcggcgg cgacaccgag
     3001 gccgccaagc tcctcggccg cgaataccgc gcgccgtgga agctggaggc gtgagggaaa
     3061 aaggtgcgcc acaggcgggc tgtccccagc ccgccgccag cggaggtgca gggtacaaag
     3121 ggcgcggcag gcatggttca gcgcggggtt ggaggttacc gggtttcgcc gcctccccgg
     3181 cggcaaaaac acgcgccccg gcggacagcc gcgcggagga cccgcgcgga catgagaggg
     3241 agcgctggaa aaacttgctc ccccgcttcc ctcccaggtt cagaaggccg gaacccttgt
     3301 tggatcgcac cgttccccat tggaaatgcc gcattcccaa ttggaaatgc gccgtccccc
     3361 attggaaatg ccgcgttccc aattggaaat gcgccgtccc ccattgggaa agcagcattc
     3421 cccattgaga aagcgccgtc ctcaattggg aaagaggcaa tccccattgg aaacgcgatg
     3481 tccctcattg ggagtgtggc gttccccatt ggaaacgagc cgtcccccat tggggaaacc
     3541 gcattcccca ttggaaacac ggcatctgaa acatcgagtg cgcgcttccc aatcgtgaat
     3601 cccatgtcct caccgcagaa acatgaggcg ggtgaatgag gcggcggatc ggattcatcg
     3661 cggtaaaaaa atcatttcac gcgcaattca gtacgacatg cggatgccgc gttagccaga
     3721 agaaattgag tagtggcgca gcccagcgct ttcacgcctc agcctcagcg tcaaaggata
     3781 caggacccct gaccgggggc gagccttcga ctgcggattg aggttgtggt ggtcatgacg
     3841 gctggaactc gcggcgggtc aggggttttg tgctccgact tgttgggcat ttaccatacg
     3901 tgggggtgtt ccgcctccca ttcatcgatc aatttcttgt cggttttctt cgatgggtcg
     3961 aggagtctga aaatttcatt tcggcgctcg ccaccaagga cgttctcacc ggtccagcgg
     4021 ccgcggtaaa agtagaaaat cttggcaccc gggtagccgt cgcgggtggc gaagtagtat
     4081 cgatatgcag tgtccgccgg tggtccttct ttggtctcac cagttgcccg actgatcctc
     4141 gcaggctcac cgagggtttg gacaatcgaa tcagtcgtga gttctttgcg gttcagatcg
     4201 gcaaaggagt cttggtagcg aaagccagcg cccgcacaac ctacgatcat catcgaagcg
     4261 atgaatgccg tcagcgcgca ccttctcgaa tgcgctgggt tggaggacgg atcaaaaggc
     4321 atttttcggc ccaacgtcaa gcatgaggcg cggctgaccg ggagcgagcg ctcgatgcgg
     4381 gacaggggtt gaaattgggg agaatcattc gaactcgcgg cgggtcagcc gttgctctcc
     4441 atgcgcttgt tctgccttgc ttcttcggta gcgtcgcaaa ggtgtgccat acctaaagaa
     4501 acgaggcgat tcaaaagggc cagatctgat ccgcagtagc gtattgcctg cacgcatgac
     4561 cccgccatcg cttcaattac ggtatccaat tcttcctgcg tctgcggctg cttgtagacg
     4621 tggcagtatt tgtcggcgtt acatggggca tccttcatct tgaggcactt cggcgctaac
     4681 tcaggaggaa aatcgcaaat gatgcactga tccttgacga cataaaacgg gcctggaaca
     4741 gcgtcagcct ctcgctcata gagcggctcg accatctgtt gtattgactg attgtccacg
     4801 actaaggcag cggatacttt ttggcagaac gttgtttaga cacacgaatg tatcgtcgca
     4861 cgggactcga cgagagtttg gtgtggatga ggaggaagat tcgtccgcga cggggccggg
     4921 agtgagaaat cttttttcat gatgggaacg agccaagcga aaaccgtgcc cggcgtcaag
     4981 accttggaga agaaaatccc agcagctccg ccgcgccagg gatgttgcgg cgcgccgcgt
     5041 ctttgacgcg cattcgcgcc tgccggggtg gggcggctgg tcctccagcc gccgcgcgcg
     5101 tgcgccaagg gtgcgaatga cctggcaggg tggttcggac ccgcagcctg cgcacgcggc
     5161 gcgttgggga caacgcgccc tggcgcgttt gccaatgaag cgcggggttg gcttcctgct
     5221 cgctgatctc gaatttcgaa tttcggtttt cgaatttctc gtctctaatc ccctgtgatg
     5281 accatctccc ctgaccaata cgaaaagctc ggcacctttt acctcgggcg cggttacgac
     5341 ctcggcgcga agcagctcca ggatgacctc gtgctctacg actcgaagga tctcgtcacg
     5401 cacggcgtcg tgctgggcat gacgggttcc ggcaagacag gcctttgcct cgctttgctg
     5461 gaggaggcgg cgatggatgg catcccgctc atcgctgtgg acccgaaggg cgacatcggc
     5521 aacgcgctgc tcacctttcc caatctcagc gcggaggact tccagccctg ggtcaatgag
     5581 gacgacgcgc ggcgcaaggg tgttagcgtc gagcagctcg cggattccca ggcgcggatg
     5641 tggagcaagg gcctcggcga atggggccag gacgccgcgc gcatccgccg cctgcgcgag
     5701 acggtggaca tggccatcta cactcccggc agcagcgccg gtctccaggt cagcatcctc
     5761 agctccctgg agtgccccgg ccccgagctg atggaggaca cggagattct cgccgagcgc
     5821 atcgaaagca ccgtctcctc gctgctcggc ctcatgggcg tggatgcgga ccccgtgcag
     5881 tcgcacgagc acatcctgct ctcgaacatc atcggccact gctggcgcgc ggggcaaagc
     5941 gtgagcctgg cgcatctcgt gcgccacatc cagcagccgc cgatgcgcgc catcggtgtc
     6001 gtggatgtgg agaccttcct gccggaggcc aagcgctccg cgctggcgat gaagctgaac
     6061 aacctcatcg cctcgcccgg attcgcctcc tggctggagg gagagccgct cgacatccag
     6121 cgcatgtttt acaccgccga gggcaggccg cgcgtcacca tcttcaacat cgcccacctc
     6181 ggcgacaatg agcgcatgtt ttttgtctcg ctgctcatga accagctcct cggctggatg
     6241 cgccgccagc agggcaccac ctccctgcgc gcgctcttct acatggacga gatcttcggc
     6301 tacctgccgc cgacggcgat gccgccttcg aagaagccga tgatgatcct gctcaagcag
     6361 gcgcgcgcct tcggcctcgg cctgctgctc gccacgcaaa accccgtgga cctcgattac
     6421 aaggcgctct cgaacatcgg cacctggtgg atcggccgcc tccagaccga gcgtgacaag
     6481 gcgcgcctgc tcgacggcct ggccggcgcc gccggggaaa aattcgaccg caaggccatg
     6541 gagaccgcca tctccggtct cggcaaccgc gtcttcctca tgaacaacgt ccacgaggac
     6601 ggccccgccg ttttccatgt ccgctgggtc atgagctacc tctgcggccc cctggcgcgc
     6661 gaccagatca agcgcctcat ggacccgctg cgccccgcca aggccgccgc gcctgccgcc
     6721 gaggacgacg gctttttgcc ccccggtgcc actgctgcac ctgcggcaga aaggaaaacc
     6781 cacaagccca agctgcctga aactgccgcg gaatacttcc tgcccaccga cacccccgcc
     6841 gactcgctct gctatgtgcc tgccattctg cgcagcgcca cggtgcagtt cgaggacacg
     6901 aggcggaaga tctccggctc cagccgcatc actctcgtga accccataga caccgccggg
     6961 cagcgcgtgc tgtgggacaa gttcgtcgag ctgccgcgcg actcggagcc cgcgcgctgg
     7021 cacactgccc aggtcgaggg agccgagttc gccgacctgc ccggcgcggc cttgaagtcc
     7081 agcacctaca ccagcatcaa gaaagactac gccgactggg tctatgccaa ccacagcatc
     7141 gaagtcggct tcagcccgct gctgaacacc ggctcgaatc ctggtgaaaa gctcgatgaa
     7201 ttccgcgccc gcgtggccca caccgcgcgc gagctgcggg accaggccat tgaggaattg
     7261 cgcgagaaga ccgccaaatc cctcaaaacc ctccaggacc gggaaatgcg cgcgcagatc
     7321 aaggtggacg cccagaagtc ccaggccacc agcgccaagc tctccaccgc cgtttccctc
     7381 ggcagcagcc tgctcggcgc cttcttcgga cgcaaaagcg gcatcggctc cctgatgaaa
     7441 accaccacgg tgaccggcgc cgcccgcgtc atgcgcgagt cgcaggacgt ggccaacgcc
     7501 cagaccgaac ttgcccgcgt gcaggaggac atcgcccttt tggaaaaaca gaccggggag
     7561 gaaaccgcca gcatccgcgg gcaatacgat cccgccaccc tcgcgctaga aacgctcaag
     7621 ctcacgcccg tgaagtcccg catccaggtg gacgccgccg gcatcgtttg gctgccgcat
     7681 gagcgcgcgg gggactccct gcgcccggca ttctgacacc gaaaggactg cctcagagtc
     7741 tcttgcgcca gcccccgccc aaaatacccg ttgacggaaa gccgcgcttt ttgttaaaac
     7801 cgcgaaaata aatcccgggg aaaattcaaa aaatccgccg gggttttgtc atgcccccac
     7861 ccaacccgcc tcccgccatg tccagccgcc tcacccagcc cctcgtttcc tcgtggaaaa
     7921 ccgtggccgc cgcgctctgc cttttgcccg tgctccacct gcaagcgatc acggatttca
     7981 acaaatggcc ggcgggtcag gaacccaacg ggcagaccaa tggaccggac ggactggacg
     8041 acgtctggca gtccatcttc aatgcctgga acattgatcc tcagggggat gaggacgggg
     8101 acggctgcag caatttgatc gaatccattg ccggaacaaa cccttttctg gcgggggact
     8161 gcctcagaat gggcgacacg atcattgccg ccagcacggt ggttttcaaa gtgaacgcca
     8221 aggccgggaa aaaatacacg gttcgcagcg atgataatcc gaatggggcg ttcaccgcgg
     8281 ttgagccgtt gctgactcca gtggcggcgc cacattttct ggccgcgtcg gacaatccga
     8341 acttgactct cagcatcgca aaaaacggcg gcaccgagaa attctacaaa gtggaagtca
     8401 gcgatcatga caccaccggc gggggagttt ccgactgggc caagctgaaa ctcggcatga
     8461 accccgccct ggcggacagc aatgacgacg ggatcagcga tgcggaggag attattgagg
     8521 aacttcaaat cccggacgag gtgacgatca ttgccagcaa gtccttcgct tccgaggacg
     8581 gcccggactc aggcaccttc acggtcagcc gcagccgcag cctgctcggt gccgcggcca
     8641 actattcggt cagcggcact gccgtcgccg gggtggatta caaccgcacg cctgttggaa
     8701 cggtgacatt cgcccccgga gtgaagacgg ccaccatcca tgtcaacgtt ctgcccgact
     8761 ctggcgtgga aggcagcgag tcggtgaccg ccacactcac caccacgacc ggggaggcct
     8821 atccgccgcc ctccatcggc ttccccgaca gcgccacggt gatcattcaa aacaacaccg
     8881 ctcccacggg caccggactg ctggggcggt attatgatca cgccagcacc atttacgccc
     8941 acggggccaa ttttggtttt acggccaact atgcttacac gcgcgcgggc acgagccctg
     9001 atttcacggg aaccaccgtc gtgacaccaa ccgctggcag cccttcgggc aaccttgcga
     9061 atctgcaggt ggggcatgtg gtgaggctga cattccttgg cggcaatctg aacactgcgg
     9121 cttacaacca tctggattac acggttaccg ccaagacggc gacaaatttc accgtccaga
     9181 tgcctccggg tgcgagcctg cccgccaaca gcagcagcac ctgcaattac agcattcaat
     9241 ccatcctgca cccgccagta atcgagaggg ttgaccctac tgtggccttc gagtggatgg
     9301 gcggcacgcc gaacggcaac tccatcagcc ccggcaatct ggcggacaac tggtcctccg
     9361 tctgggaggg ttacctgcac cccacgacga cggggaatca ccgcctgcaa ctggacgcgg
     9421 atgacaaggc ccgcgtgctc ctggatttga acggcaatgg tgttttcgac ctgcctggcg
     9481 agcagatcgt cgagcacggc tgggacacgg cggccaccgc cagccctgag gatggtgtgg
     9541 cggatgacga gaccatcggc acctttaaaa tcagcgccaa tcacgccctc accatcccgg
     9601 catcggcggc tcagagatac aaaatgcgcg tggagatggt ggagacgacc ggggaggccc
     9661 ggtgccgcct gcaatggagc atcaacaacg gcaccttcgc caacatcccg tcggcgaaca
     9721 tgtttaccca cactcagggg atgacgtcga actacgattt caatcgcctg accacgggaa
     9781 gtcccatgac aggcacgatc acggtgcggc ttaacgcgca cgggttctcg gtcggcagcc
     9841 ccgtcgagct ggccttttcc tccggagtgt tgttcacccc cgcgaacggc aatttccacg
     9901 gctcctacac agtggccaca gtcaacagcg ccaatcagtt tgtcgtgaac atctccgcgc
     9961 cgtcactgcc tgccagctcc aacaattccg ggggtggttt catcttaaac cggccggcca
    10021 gcaccacagc gggtctgctc aactgggtct ggcaaaactc aagcttctca ggggctccag
    10081 gccgtgtcgg caccaacacc aacggcacca atgacagcaa caacggcctc tacggcaccg
    10141 gcacgccgga tgacgcgctg atcaatccgg acagcttctc cgtgcgctgg accgggcaga
    10201 tgcagccgca gttcaccgag gagtacacca tcagcgtgtt tgcggatgac ggctccaggc
    10261 ttctcatcaa cggcgtcgag caggatctca agatgctgcc ctccgccaac acgggcggca
    10321 gcacatattc gtatgacagc gcaactggag acgcggtggt cacttacacg aatgcgatca
    10381 tcaagcccgg cagcattgtg gctggcgagg ttttgcgctt tgaccccgcc aacggagtcc
    10441 tggcgctcgg caacggcagc acctacacct atgacagcgt gaccggggac gcggtcatca
    10501 attattccaa cctgaccaat gtcaacccgg gcggttttgt cgtcggcgag acgatcgagg
    10561 tggacccgac caacggcacg gccaccagcc tggcgctgct tccttatcaa atcaccgccg
    10621 ccaccgccac caccttcacc gtgaactttg gcgcgggcgt cttcgagtcc ggcaccggca
    10681 gcgtcaacgt cagcgacaat tcggaccgcg tggtaaccgc cgccactgcc accaccttca
    10741 ctgtgaactt cggtgctggc aaacacctca acggcacggg caccatgaac ttcaacttcg
    10801 tcaacaagcc cgtcaagccc tgggcctcga acggcaacga gcgctatgtc cgactgccgc
    10861 tggtcggcgg ggtgcgttac gacatccagt tggaatatta tgaaagcggc ggtttctgcc
    10921 gctgctggtt gtattggatg agtcccagcc agccgaagca ggtcataccc acggagcgtc
    10981 tctatccctc cagtgatccg gtcatcgcgc cggcctctca tgtcagcccg acggatgcga
    11041 cggcgcttgt gggcggcaat ttctcgcatg ccgtggaagg gagcaacggc gcttcggtga
    11101 gtcttagcgg ggcgcctgcc tggctgacct ttgccaatgg tgtcctcagc ggcacgccgc
    11161 ctcctggatc ggcggggatt tatcaaatcc tcatcacact gacgaacgcc gcaggcacca
    11221 gcacctcggt cttgaatctc accgtcgaac aatccggagg ctccgtcgag cgcgaggtct
    11281 ggaccggcat cgccggcacc tcggtctcca gcattccggt tggcaccacg ccgaacagca
    11341 ccaacaccct cacctccctc gcggcgcaga ccgattttgg cgacaactac ggcgcgcgca
    11401 tccgcggtta catcaccgcc cccgtgaccg gcaactacta cttgtgggtc gcggccagca
    11461 actccgccga actttggatt tccaacgacg acgatcccat caattccttc aagcgggctt
    11521 cggtgctcag cggccatagc ggtggctggt cctggaatgc cgagccttcc caaaaatccg
    11581 cgtggctggc cctcaaacag ggcaaacgct attattttga agttctgcac aaagcaggca
    11641 cgggagccgg tgacaacctc gccgtcggct ggtccaaacc aggtgaaaac gaaagcgctc
    11701 cagcggagat cgtccccggc catgtgctta ctcccttcgt cccgcccgcc cccggttcga
    11761 cccccggcac cctttatgtg gccaccatgc tttcccaggg tggtgccatc accaacggcg
    11821 ttggcaccgc caccctccgg ctcagcgagg acgagaccgt cgcccacgtc gccttcaccc
    11881 acaacggcct caccggcccg gtcaccgact ggcacgtcca ctccgacccc ttcctgaccc
    11941 atcagtccgc catcatttat gacggcaccg agcccttgcc gggcgacggc ccgcagcctg
    12001 acggcaccca caaatggaac attgtcaccg cagggacgct ctccgccgcc gagatcatcg
    12061 agctgctcaa gcagggcaag gcctacatca acctgcacac cgccgaatat cccaacggcg
    12121 aaattcgcgg caacttcacc ctcgccaacg gctcccgcac cttcacgccg ccgccgccgc
    12181 cgcccatgtg ggccgatgac agcaacacga acgcgggcgc ggcgcgtttc cttacccagg
    12241 ccagcttcgg tccgaatgtc gccgacatta ccgcgctgaa ggcgctcacc gccaccggcg
    12301 gctccgccca ctatccgccc agccgttatg agacctggat tgacaaccag ttcacgcagc
    12361 cctggtcaga tcatctcacg gaggttctgc gcaccgagca atccagcgcc cagggcggtg
    12421 ccttcgagga aattctcact ttcaattcct ggtggcgcag gtccattagc ggcgcggacc
    12481 agttgcgcca gcgtctggct ttcgccctca gtgaaattca tgttgtctcc ggccaggggc
    12541 cgctcgacaa ccgcgccgag gccctctcct acttctatga caagctcggt gagaacgctt
    12601 tcggcaactt ccgcgccatt ctagagacca ccacgctgac ccccaccatg ggccgttacc
    12661 ttgacatgct gcgcaacgac aagcctgacc tttccaccgg ccgcatcccg aacgaaaact
    12721 acgcccgcga gatcaagcag ctcttctccg tcggtctcta ccgcatgtgg ccggacggca
    12781 cgctcatgct gaactccaaa gacagcccga ttgacactta cacccagagt gaaatcgtcg
    12841 gctactccca tgtcttcacc ggctgggact acggctatga cggcttcaac cgcaccgccc
    12901 tcaacgcccc ggccaactgg atgcgccaga tgcgccccac cccggcccgt cacttcaccg
    12961 gtcccaagct tctgctcaac aacgaggtgc tccccggcct gcgcaccctt ggcggccagc
    13021 cgctcgaccc ctacgccaac cacatcagcg cccagttcaa cgatcctgct tacaaggccc
    13081 tgcctgacta tgagctggct gtctcccacg accagctctt caaccacccg aacgtcggcc
    13141 ccttcatctg ccgccagctc atccagcgcc tcgtcaccag ccatccttcc cgcgactacc
    13201 tctaccgcgt cgtccagaag ttcaatgaca acggctcagg cgtgcgcggt gacatgaagg
    13261 ccgtgatcaa ggccatcctc ctcgattatg aggcgcgcag cccccagatg ctgcaaatcc
    13321 ccgcctacgg caagcagcgc gagcctgttc tccgcgtcgc cgccgccgcc cgcgccttcc
    13381 gcaaggctcc ttggtccgga acctactccc agagcgccac agatcccacc gatccgcgcc
    13441 ggatctacat tactaccaac gatcctcacg ggttccccac cggcacgagc aaccagcttt
    13501 tggagttcac ttccggcaat ccggcaccct ggacgggagt ttactctgtg gcacagacgg
    13561 gtgccaacac tctcagagcc accgccaccg gctgggcaac aggcacctac agcatccctg
    13621 caaattccac cacctgcacg atcaccatgt ccaaccattg gattcaggtc ggcaatcaat
    13681 tcttcattga tttctacacc aacaccagcg ggacggctaa cggctccgcc atcgaccagc
    13741 aagtgtacac cgttgccacc aaccccaatc cgagcagcgg cggcaacaac ggctcaagct
    13801 tcacttttga aattcccctc gcgcagacat ccggcacgct gcgcactggc agctgcatga
    13861 tcccgcgctt ctcgcccggc agttaccaat attccaacaa caacaccggc gtgccggtgg
    13921 accagcaggg cacttacaac cggctggtga ccatggacac ggccaacaac acccaccacg
    13981 agctgaacgt gggcgaccaa gtgcagctca acttctacgg aggcaacccc acgcccaacg
    14041 acgccgtcgt caccgtccac agcatcgtgg atgagaacac ctggaccttc ctggccaaca
    14101 acaccgcgat gggtctcggc aacaacgccc agggcttcaa cagcgtctat cagttcccgc
    14161 tcaagtccct gcctctcacc cgcaatggca acgtgggtgc gcgccccagc accttctcca
    14221 tgggcaacac cacgctggag cttgaccagg cgccgatcaa ctcgcccacg gtgttcaact
    14281 tctacctgcc cgacttcaag ttcccgggtg cgctcgcctc ccagggcatc accacgccgg
    14341 agttccagga gaccgccgaa accagcgtca tccgccaggc caacttcatc tacaacggcg
    14401 ccttcggcgg cggtttggag aacatcagca gcttcaacaa cggctccaac gccctgctca
    14461 tgagcttcag cgactggacc accggcacaa gcgcccctga cctcggcctt ggcgtggcag
    14521 tcacgcccac cgtgccctgg acccacaacc aaaacatcgc ccggttgatc gaccacttga
    14581 gtgtcctgct cacagccgac cagttgtccg cccaggccag gcagatcatt cgtgattttg
    14641 tctccctgcc tatcagctcc atcagcactg gcaacccctg caccatcacc acggctcgat
    14701 ctcataatct gaccacaggc acgactgtct gcatcagcgg tgtgaccaac ggcacctaca
    14761 acggcagtac tacgcttctc aacagcacca ccaccggcta ctccgccaca gtgaccgggg
    14821 agacgacctt caccataccg gtgaactgca cctccgcgcc aaacgcctcc ggccttgcca
    14881 acgcccacgt ctccaccgtc gtctacaacc aggggtccaa ctccgcgacc aatccttcca
    14941 tcgccagccg caaggaccgc atccgctcca tcatccacct gatcctgacc agccccgact
    15001 tcaccatcca gcgctaaaag cttatgaatc ccccgaaagc tccgaacatc ctctccacgc
    15061 gccgccgcgt gctgcgcaac gccgcttacg ccagcatggg cgccaccgcc atcggcagcg
    15121 cgttgcgcga tctgcgcctc atcaactccg ccatggcggc cgggccgatc acggattaca
    15181 aggcgctggt ctgcctcttc ctggccggcg gcaacgactc caacaactgg atcgtgccca
    15241 ccgacacgcc gacgtatgac gcctatgcgt ccatccgcgg caatttggcg cttccctcag
    15301 cctcgctgct gactctgcga acgggacccg gcggcagtga tcccgcgtat gtggacggag
    15361 acggccacac cctgggtttt caccccacct gccaggaatt gcgcaccctg ttcggcgagg
    15421 gcaagctggc cgctgtcatg aacgtgggca ccctggtccg ccccaccacc cgcgcgcagt
    15481 acctcgccaa ccccactttt taccgcccgc cgcagatctt ctcccacagc gaccaggtca
    15541 cccagtggca gacctccatc cccgaccagc cgcccatcac cggctggggc gggcgcgtgg
    15601 cggatctgct gaactccgtg gccaacccgt cggggaatat ctccatgtcc gtctccctga
    15661 atggcaacaa caccttcgaa atcggcaact tggtctctca gtaccatgtt tcccaaactg
    15721 gtgctgtcac gctcagcggg gccctgatgg ccggcagcca gccgcgtgtg aaggcgatga
    15781 aggacctcat cgccctcagc cacccgaacc tccagcgcca ggcctacagc ggcgtgctcg
    15841 acagcgccat cgtcaccggc gacctgctca acagcagcat cgccgccacc atcgaggtcg
    15901 cccagggcgg cacctgggtt tggaacacgg cgttccccgc caccggcctg ggcaaccagc
    15961 tcaaaatgat cgcccgcctc atccaggcgc gcggccctgc cgccttcaac atgaaccgcc
    16021 aggtcttctt ctgctccgtc ggcggttatg acacgcacac cgcccaggtg accgacccgc
    16081 tcaatgccgc caaccagttc aaccccacca ccggcaccca caccaacctg ctcaacgagg
    16141 tcagcgagtg catgttcgcc ttccagcgcg ccatggagca gctcggcgtc tccgacaagg
    16201 tcaccacctt caccgccagc gacttctccc gcaccttccc caccaacagc cagggcagcg
    16261 accacggctg gggcgcgcac cacctcatca tgggcggcgc ggtcaaaggc ggcgaaacct
    16321 acggcagcct tcccaccttt gccatcaacg ggcccgacga caccggcacc ggccgctgga
    16381 ttcccaccct ggccgtggac cagtactccg ccacgctggc caaatggttc ggcctggaca
    16441 gcaatgaaat tgccgccgtc ttccccaacc tgagccgctt ctcatccagc gacctcgggt
    16501 tcatgctgtg accatggcgc ggaagctctg gcccgttctc ctcgtcctcg ccgtcgcggc
    16561 ggttttcctc ctctggcggg gcggggaaaa gcccgccgcc cccgccaagc cagccgctcc
    16621 cgcgcggacg gcggccaatg agcctgcaaa tcccctgccg ccggtggcga gag
//
LOCUS       DAOMYJ010000109        16585 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Prosthecobacter sp. isolate AMR_MDS_5629
            NODE_1209_length_16585_cov_24.981972, whole genome shotgun
            sequence.
ACCESSION   DAOMYJ010000109 DAOMYJ010000000
VERSION     DAOMYJ010000109.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867850
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prosthecobacter sp. (wastewater metagenome)
  ORGANISM  Prosthecobacter sp.
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Verrucomicrobiaceae; Prosthecobacter.
REFERENCE   1  (bases 1 to 16585)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16585)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.30
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:34:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,742
            CDSs (total)                      :: 4,684
            Genes (coding)                    :: 4,676
            CDSs (with protein)               :: 4,676
            Genes (RNA)                       :: 58
            tRNAs                             :: 55
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16585
                     /organism="Prosthecobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1209_length_16585_cov_24.981972"
                     /isolate="AMR_MDS_5629"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1965333"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            81..452
                     /locus_tag="PLP58_12330"
     CDS             81..452
                     /locus_tag="PLP58_12330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ09354.1"
                     /translation="MNEKIAAIETARMSLDELRPHPRNPRKHPEPGSKMWEIMKRSLD
                     RSYFEPLVWNRRNGCLVSGHLRLKVLKEMGYAQADVSVVDLAEGSANLRSSDNIEQIV
                     ITNDPDTLAFHTAWMEELPAV"
     gene            449..1519
                     /locus_tag="PLP58_12335"
     CDS             449..1519
                     /locus_tag="PLP58_12335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ09355.1"
                     /translation="MNELTPDLAAKLIGADLRNLVKKVSDGQSLTQAEREMMERAVID
                     GTLPQELQEARLAALARAYVLGKSLTKSQAAELSSVIPVGKPIAKRLTRQTYRYKLSH
                     YVPILGIDKDAHGHPAKDPERKIKRWIEKGRQQVPPDLPPLDAPAQMAEWWRRNMEWR
                     VPEYLLRLENSAGEQTGSKPAEPCVEPASVDRPAVTPSASDVEAPVNGEKIEAPMMFD
                     DAAEMTADIGLNQIRSLVSTLYKRMQTAFEAGRISEGQSYMRQWKEAVAMLRQWEKDI
                     VKIQEGKGEVLRTRVINTELGRIFNIISQSFFNSLMGLLDVHAPQIPAQERRQLALQQ
                     RDKCFAHLKSGRFSAIYQPGHE"
     gene            1512..3458
                     /locus_tag="PLP58_12340"
     CDS             1512..3458
                     /locus_tag="PLP58_12340"
                     /inference="COORDINATES: protein motif:HMM:NF017673.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase large subunit family protein"
                     /protein_id="HRJ09356.1"
                     /translation="MSDLFQRAQAMRYAGSPENDFIVSLAGDYMQETPTEPVWQWAAR
                     NVYLDEKMTSEPGYFDPTKTPWTKEWQELPMRPEVREAVAMKSSRTGFSEGSLNILRW
                     MVKHWPGNALYSINSDKKAREVAERRILPSIERTAGAQLSEDPDDNTLSKISLRNMDI
                     LISGSGSSGPFMEIWYRLIVLDELSKHAKRADGTTTYDQAKSRQNDVADGLLMALSVP
                     EEAGGIIDLKYTNGTQKKFLVPCPRCERRIELLFDYLLADHCKDGDHRYNLDLVVAET
                     VYQCQLCGQPIEEKEKYAMVNEGIWVPTSNDKRRRSPAGDYVPPEPGVESYQISDLYS
                     LHKRVTWGMLKKLWLQAYVINPTDEAKKYVITNHGGLPWEQEATNVTKESIEALKAGR
                     IEEQRVKAADGTESTIKVHLGQPFRMAYRDGEFNARLPFCPAMLFMTTDKQFDYLKYL
                     VFAWSNSGESYLVDCGRLQDEDQLWRLRTREYKVEGRKRPRHIDSGLIDCGHRRDEVY
                     KACLLHQMQPCIIHPGGWKIFPARGSGENEGHAGKLLMLKKDYLDSHEITVRVFYDHG
                     IKNQFYLGQIQKRDEPRLWLPVDLPSAISSEWTAEIYDKAEKKWKHDKQKRGPNDYGD
                     CGKMQFVGKIEFGGALQNIHWPDD"
     gene            3492..3710
                     /locus_tag="PLP58_12345"
     CDS             3492..3710
                     /locus_tag="PLP58_12345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ09357.1"
                     /translation="MATNFQKHQLDVMAQAKRLCEDAGCLVKVERLGVLIIVMAEYGE
                     KEAEQLFLEATEPRQDRGGEEGRRGGQG"
     gene            3836..4195
                     /locus_tag="PLP58_12350"
     CDS             3836..4195
                     /locus_tag="PLP58_12350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ09358.1"
                     /translation="MARIDTHASGVPETTTNPELLIQGFLRQARLQVAPVAWLNERHA
                     EALSAVAAGDEFVTSTTYQGQASAALRGVPATMLLQIYEACLQRLEAEESAASGIYES
                     PGSVRHADFSGFPSTLG"
     gene            4192..5799
                     /locus_tag="PLP58_12355"
     CDS             4192..5799
                     /locus_tag="PLP58_12355"
                     /inference="COORDINATES: protein motif:HMM:NF016991.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HRJ09359.1"
                     /translation="MMSTNDKRRVILGPQGDVIRRLEAKDPALEAVGMYRSFQGTDVG
                     SRDRGYVYVPTLQTLKEVDEWSHVELMRRGRYIYNSGGGLVHRGVNGVARMVCGTGLF
                     PYPGSPNKAWNAKVRRLWMARCENKNTFDLSRKFTCGSAQQGIVRAKIKDGDMAPVLA
                     RDESGRLRCMFYEAHQIGQGGKREAPGERWHHGVRLGRHNEPLAYRVVSVDDIGGGQT
                     EVEVPAGNVLFCATYERFGQVRGLSRFYPVINKVLDRGEILHALTKGIKMREQIGFAI
                     EQQLAVQMIGGPGGWGAGIPQPTSQVALPTGEKITLEKFFGAGEARELKPGQTFKLVE
                     SDHPNENVREHLETLTRDVAWALGYSPELLWNITQLGGANTRFIMADAQSQIESEQQE
                     LVEQFLGPWYLAWLRDMIEAGELEDVESWELHSWVLPKRLTVDFGRDGKLHIEQAKRG
                     HITLKALHGFQGDQWEQEVDQYLDERLYIKEGMAARGLTWAEAFPEIGAGTTAAQEQL
                     DDDEAARQAEETQAALQRAVNKPPIRI"
     gene            5845..6798
                     /locus_tag="PLP58_12360"
     CDS             5845..6798
                     /locus_tag="PLP58_12360"
                     /inference="COORDINATES: protein motif:HMM:NF013506.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S49 family peptidase"
                     /protein_id="HRJ09360.1"
                     /translation="MHSETLHLPHLARQFYFEPLALEESAMLACHMYLWPRITGQAAD
                     LSPLSEAKESNPGSIKYGGGLAHMRKRMAEPNMQSNGPFAAPTILDPAYYWTVEGKPS
                     MAVIPINGMISKGISPFMEFCMGAVNPDRISHALRQAVEAKGIKQIVLDIGSPGGRTT
                     FTPELAAQVKAAAATRGKTVYAFTDTMIASAAEWLASQADEVIMTASAQVGSIGTYLA
                     FLNPKVAMQMQGYSLELFSKGTHKGLGLPGRDLTQADREYLQGTVDSLNAQFVSAVKA
                     GRPKASEEALTAAKMYDGPDAVRHGLADGIVGSWDEFVALV"
     gene            6876..7526
                     /locus_tag="PLP58_12365"
     CDS             6876..7526
                     /locus_tag="PLP58_12365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ09361.1"
                     /translation="MVSLLILAVLCAPLLLAVPAEPGLALCGIPALMLPLRTPADDSG
                     EGGGGGPVNVADALAKLEDKTLPLSQRLSVAAAALRGIDPTQQLAGTKLKLDEASAEV
                     ARLKSELDGSRMQLAAREKDVADLEARVAELEKAHADLAAKEQDLEKRAAAKAQERMA
                     SLGFPAGKLPAPSADDSGLKDVPKTEAQLTEALVKCKTQKERSALLREYRASVHAA"
     gene            7575..8741
                     /locus_tag="PLP58_12370"
     CDS             7575..8741
                     /locus_tag="PLP58_12370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ09362.1"
                     /translation="MSNTIASELQLTTVLDSALVAFRRAILPLQQFATSYSNVPLRGD
                     NTMAIPYYPLATSDSVTRASGGSYKALATSTTTQAKKVVINKNKVQAISFTSEERSRQ
                     PMFNPEMHGRLKGEKLAFDIIADIFSPVRHSVFTGATISATTAGNFDENDVADLAQKC
                     MEDYWPEVGRSLILNPAFHFNLLKQGQIIDASKSADPLAFREATIRRILGFEELGTAG
                     LPTNNGTAFAVLGEADDDTFTAAAHGLLDGDRVIFPTLTGGTGLTAATTAYFVRDAAT
                     NTFKVSATIGGAAVNFTSDVTAGTCRRYENIVGVAAVPSALLVGFAPVEPTPAVRSQL
                     FDYRVVEDRYSGIVLEYKHIGYGDTDEEVQVIEAHYGDALGETAAMKLITTPLS"
     gene            8771..9160
                     /locus_tag="PLP58_12375"
     CDS             8771..9160
                     /locus_tag="PLP58_12375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ09363.1"
                     /translation="MKLALLIGLDAEKRSGFPLTPVAVPFDEALRAFKGMVAAGVAPA
                     PHVQLWSSNGLVKQARFKKVQAPKAAKGSKTELLKRISELEARVVELEAQTPTGAAQT
                     PPLTSLEKEPEPSLEDPAPDSPLVPKT"
     gene            9263..9529
                     /locus_tag="PLP58_12380"
     CDS             9263..9529
                     /locus_tag="PLP58_12380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ09364.1"
                     /translation="MKLEELTQDLDRKNGRRVIRVTDAGGDERVTMTLEFESNTSLPD
                     EAPRYGPVDHPPANSKVREYALELLAALHKGVWRLDLTGGPGAA"
     gene            9559..9858
                     /locus_tag="PLP58_12385"
     CDS             9559..9858
                     /locus_tag="PLP58_12385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ09365.1"
                     /translation="MQRGRAASPFTGEVTVIMGATRHRVCGLVSSGDNKAQVDEFGLE
                     HVRTLEAHLPKCRLPRRPDASLDAVEYLGRSYKLASIKGGDACSDVWVISAVAPF"
     gene            9901..10359
                     /locus_tag="PLP58_12390"
     CDS             9901..10359
                     /locus_tag="PLP58_12390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ09366.1"
                     /translation="MDFLTATQAGTPDVEIPKVAFDGGSVPTPLCIVVVAREAQEKKT
                     TVRRMEITVNLITWLKAADAGAASVDTQTTREDAGEIVRGLEDRLRDDAAWNTFIAGV
                     AAGENAARLQGWSIVCRRFEGVGSPMREAETRKLTYPIKLRYSLAVSRGV"
     gene            10398..10886
                     /locus_tag="PLP58_12395"
     CDS             10398..10886
                     /locus_tag="PLP58_12395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ09367.1"
                     /translation="MKHLLSAALLALALLLPTAALCADVSVTAGNVRTSDYAVIAPGV
                     AGETITAGQVLFLSSADDRYYLADVNAAGETLIAGIAVSGAAAGQRLLVCIEDPDFTP
                     GFTLSTSAPVYVASATAGGIAPVADVTTGWYCTVIMVAKSTTKAAFRARGLASGSAAT
                     AP"
     gene            10927..11388
                     /locus_tag="PLP58_12400"
     CDS             10927..11388
                     /locus_tag="PLP58_12400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ09368.1"
                     /translation="MPFDPVQVYGALPAYDRREFAESGFLTETVKFTPEQEVKQKKAH
                     AVAALGQVAQVQIWRGSLAIEIAGALVADANGLVHGLPAGYVGQAVTTCAHFAAGTAE
                     VPAIARHGYTRDPEKLLLIETASTDLGEEEPAATLGLRYFPFVDATPLLPA"
     gene            11512..12366
                     /locus_tag="PLP58_12405"
     CDS             11512..12366
                     /locus_tag="PLP58_12405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ09369.1"
                     /translation="MALTPQRAANTLAITETRLAASLRALGFPYTAETTIHERTGAAV
                     IQFLFGLSSLRFPALKLPQMLQAWRSGEMAREDPLHVLHVMMTAHECYDALLRMMKDH
                     VSHCLHLRSGGVLTVYRPGAAAPPPGARLVWTNDLNLAAACGVVGVPVLSIDGSAGQH
                     RFGLAPCGYRVLLSDGGWQAHDTATLIARDPVREDEWRLRMEATQPLHPLCLAYDALH
                     NRAQLKAELKGQGRAPMILIDEPARVIPGAHKGACLAVRQALVSADAPGHVMDQVTRH
                     MQAPPMSW"
     gene            12391..12993
                     /locus_tag="PLP58_12410"
     CDS             12391..12993
                     /locus_tag="PLP58_12410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ09370.1"
                     /translation="MKRRTQSTSAALPDPPHESESPGISAPASPPPARTGADDDSKAR
                     AAAAQREQLWDEEGTRSFAGLPLLPWSRERHTLAARLVALDLPAPALAELVRIREMLD
                     ARKDAPFVPLSFAVDLAAYLPTAEKILWLATHEADEINALRTRPTLLLQTVSEWSVSV
                     ISPELFEPACLLAHRLTSEHLRVQPMPMPNKKGKGRDSGN"
     gene            12996..13241
                     /locus_tag="PLP58_12415"
     CDS             12996..13241
                     /locus_tag="PLP58_12415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ09371.1"
                     /translation="MPATGAEYYALLSRALPGTPLHVLRWQVSLHEARALIHASGILE
                     GGSYIWPDPELSSRGRAMLRVRETLRRVRAGEWEVDL"
     gene            14488..14691
                     /locus_tag="PLP58_12420"
     CDS             14488..14691
                     /locus_tag="PLP58_12420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ09372.1"
                     /translation="MPLDDAETVNRISQILFGYREAPESEIFTRLEYLVEREKSINLH
                     DAVKGGKLKIAIEALHEALEGLA"
     gene            14842..15579
                     /locus_tag="PLP58_12425"
     CDS             14842..15579
                     /locus_tag="PLP58_12425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ09373.1"
                     /translation="MSLVIHSEAHSLAFARLVSTSKKAAVDLVRQEFGLLLTEVAKVT
                     PPYSEKMSGKKAEVQGRAAVAADIRALYGTPGDAYDAISAPAAKAAFWFLHQSGDNAA
                     ASQILRAETGTGLSPFDGGTVHGRRRPGNRRRRQRRVVYYVADTDALDVYIAAEQAHV
                     WWLASGWAPALRALGRRLPYGVERHSAPGTMRAVITDQRIELVAVDSVAFASRVRDIE
                     RQIQFALKIRTGAMQRSWDHFTRNVRL"
     gene            15588..>16585
                     /locus_tag="PLP58_12430"
     CDS             15588..>16585
                     /locus_tag="PLP58_12430"
                     /inference="COORDINATES: protein motif:HMM:NF040129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tape measure protein"
                     /protein_id="HRJ09374.1"
                     /translation="MAAIEASLRLEIAQYQAAMARARGEIDRLKARAKSGGAGLSKDL
                     FGGVMRGAAALGVTLGTARIASGIFDSVVAMESLEKMLRTTEGSAEAATQRMAELREV
                     ARLPGVGFEQAVRADVRLRSIGLTADDSKNAIIEFGNALALVGGTRQDLDGVVLALGQ
                     ISAKGKVSAEEINQIAERIPQVRAVMRAVFGTADTEAIQAMKLEANDFIRLLVAGLAE
                     LQRAQKGLQDELADISTMGMEMANDIGGELVRTLIPAFQDVLSVIAANREGLQSFGAV
                     AADVFSAVADILEATSIIGYAGAASIIGGENFFDAHRARAEEVRRANERAAPAPAA"
BASE COUNT         3268 a         4877 c         5446 g         2994 t
ORIGIN      
        1 ggtataacgt cccaccccct ttttttgcgc gcagtatcaa aattcaaaac agcccttttt
       61 tttgacactg cgccagcggg gtgaatgaaa agattgctgc tattgagacg gccaggatga
      121 gcctggatga gctgcgcccg catcctcgga atccgcgcaa gcatccggag ccaggctcga
      181 aaatgtggga gattatgaag cgttccttgg ataggagcta cttcgagcca ttggtctgga
      241 accggaggaa tggctgcctg gtctctggtc acctgcggct caaagtgctg aaagaaatgg
      301 ggtatgcgca agctgacgtg agcgtggtgg atctggccga gggcagcgcc aacctgcgca
      361 gttcggacaa catcgagcag attgtcatca caaatgatcc agacacgctg gcctttcata
      421 ctgcgtggat ggaggaactc cccgccgtat gaatgagctg actccagatc tggcggcgaa
      481 attgatcggg gcggatctgc gcaacctggt caagaaagtg tcggacgggc agtcgctgac
      541 acaggcggag agggagatga tggagcgcgc ggtgattgac ggcacgctgc cgcaggagct
      601 gcaggaagcc aggctggcag cgctcgcgcg cgcttacgtc ctgggcaagt cgctgacaaa
      661 aagccaggcg gccgagttgt cgtcggtgat cccggtgggg aagcctattg ccaagcgtct
      721 cacgcggcag acataccgtt acaagctgag ccactacgtg ccgattctgg gaattgataa
      781 agacgcgcat gggcaccccg ccaaagatcc ggagcggaaa atcaagcggt ggatcgaaaa
      841 gggccggcag caggtgccgc cagacctgcc gccgcttgat gcgcctgcgc agatggcaga
      901 gtggtggcgg cgaaacatgg agtggcgcgt gccggaatac ctgttgaggc tggaaaactc
      961 ggcgggcgag cagacgggca gcaaacctgc tgaaccttgc gtcgagccgg ctagtgtgga
     1021 tcggccagca gtcacaccct cggcctcaga cgtggaggcg ccagtgaatg gcgaaaaaat
     1081 cgaggcgccg atgatgttcg acgatgcggc cgagatgacc gccgacatcg ggttgaatca
     1141 gattcgcagc ctggtctcga ctctctacaa gcggatgcaa accgcatttg aagccggccg
     1201 catttcggag gggcagagtt acatgcgcca gtggaaagaa gctgtggcca tgctgcgcca
     1261 atgggagaaa gacatcgtca agatccagga aggcaagggc gaggtgctgc gaacgcgcgt
     1321 gatcaacact gagttggggc gcatcttcaa catcatcagc cagagcttct tcaactcttt
     1381 gatggggctg ctcgatgtgc atgcgccaca gatcccggcc caggagcggc ggcagctcgc
     1441 gctgcagcag cgggacaaat gttttgccca cctcaagagc ggacgattct ccgccatcta
     1501 ccagcccggc catgagtgac cttttccagc gcgcccaggc catgcgatat gcaggctcgc
     1561 cagaaaacga ttttatcgtc agtctagccg gcgactacat gcaagagacg ccgacagaac
     1621 cggtttggca atgggcagcg cggaacgtct atctcgacga aaaaatgacc agtgagccgg
     1681 gttactttga cccgacgaag acgccctgga caaaagagtg gcaggagctg ccgatgcgcc
     1741 cggaggtgcg tgaggccgtg gcgatgaaat caagccgcac aggcttttcc gaaggatcgc
     1801 tgaacatcct ccggtggatg gtgaagcact ggccgggcaa cgcgctttac agcatcaatt
     1861 ccgacaagaa ggcgcgcgag gtggcggagc ggcgcattct cccttccatc gagcgcaccg
     1921 caggggcgca gttgagcgaa gacccggatg acaacacgct gtcgaagatc tcgctgcgca
     1981 acatggacat cctcatcagc ggctccgggt catcggggcc gttcatggaa atctggtatc
     2041 ggctcatcgt gctggacgaa ttgtcgaagc acgccaaacg ggcggatggg acgaccacgt
     2101 atgaccaggc gaagagccgt cagaacgatg tggcagatgg tctgctgatg gctctttctg
     2161 tgcccgaaga agcgggcggg atcattgatc tcaaatacac gaacggcacc caaaaaaagt
     2221 ttttggtgcc gtgtccgcgc tgcgagcggc gcatcgagct actgtttgat tatttgttgg
     2281 ccgatcactg caaggatggg gatcaccgct acaacctgga cctcgtggtg gcggagacgg
     2341 tgtatcaatg ccagctctgc gggcagccca tcgaagagaa ggagaagtat gcgatggtca
     2401 atgaaggcat ctgggtgccg acgtcgaatg acaagcgccg gcgctcgccc gcgggcgatt
     2461 acgtgccgcc ggagccagga gtggaaagct accagatcag cgacctctac agcctgcaca
     2521 agcgcgtgac ctggggcatg ttgaagaagc tctggctgca ggcttacgtg atcaatccca
     2581 ccgacgaagc caagaagtat gtcatcacga atcacggcgg cctgccctgg gagcaggaag
     2641 ccaccaacgt gacgaaggag agcatcgagg cgctgaaggc gggccgcatc gaggagcaac
     2701 gcgtgaaggc ggcggatggc acggaaagca cgatcaaggt gcatctcggc cagccgttcc
     2761 gcatggccta ccgcgacggc gagttcaatg cccgcctgcc cttctgcccg gccatgttgt
     2821 tcatgacgac ggacaagcag ttcgattatc tgaagtatct cgtctttgca tggagcaact
     2881 cgggagagag ctacctggtg gactgcggcc ggctccagga cgaagaccag ctttggaggc
     2941 tgcgcacgcg ggaatacaag gtcgagggaa ggaagcggcc gcgtcacatt gatagcgggc
     3001 tcatagactg cgggcaccgg cgcgatgagg tttacaaggc gtgcctgctg catcagatgc
     3061 aaccgtgcat catccatcct ggtggatgga aaatcttccc cgcgcgtgga tcgggtgaga
     3121 atgaaggtca tgccggcaag ctgctcatgc tcaagaagga ctacctcgac tcgcacgaaa
     3181 tcacggtgcg ggtcttctac gaccacggta tcaaaaatca attttacctg gggcagatcc
     3241 aaaagcggga cgagccgcgt ctgtggcttc cggtggatct cccgagcgcg atctcgtccg
     3301 agtggacggc ggagatctat gacaaggcgg agaagaagtg gaaacatgac aagcagaaac
     3361 gcggcccgaa cgattacggg gactgcggta agatgcagtt cgttgggaag atcgaatttg
     3421 gcggcgctct gcaaaatatt cactggccgg atgattgaca gccgttgccg ttttgcttgc
     3481 gtagaaacat tatggcaacg aactttcaga agcatcaact cgacgtgatg gcgcaggcca
     3541 agaggctctg cgaagacgcc gggtgtctgg tcaaggttga acgcttgggc gtgctgatca
     3601 ttgtgatggc cgagtatggc gaaaaagaag ccgagcagct cttcctcgaa gccaccgaac
     3661 cccgccagga tcgaggcggg gaggaagggc ggcgcggcgg gcaagggtga ggccaaggcg
     3721 cgcacttcgg aacaggcgcg cgccgcagtg aatgcgaggt gggagaagtt tcgaaagcgc
     3781 gtggcgggct ggaaggaaga agccgcgccg gagcaggccg agccttgcaa cgaagatggc
     3841 tcggattgac actcacgcat ccggcgtgcc agaaacgacg acaaaccccg agctgctgat
     3901 tcaaggattt ttgcgccagg cgcgccttca agtggcgcct gtggcctggc tgaatgagcg
     3961 ccatgcggaa gcgctctcgg cggtggcggc gggggatgag tttgtgactt caacgactta
     4021 ccaagggcaa gccagtgcgg ccttgcgcgg cgtgcctgca acgatgctgt tgcaaatcta
     4081 cgaggcatgc ctgcagcggc tggaggccga ggaatcggcc gcctcaggca tttatgaatc
     4141 tcccggttca gtccgccacg cggacttcag cggctttccc tcgaccctcg gatgatgagc
     4201 acgaacgaca agcgccgcgt cattctcggg ccgcagggcg atgtcatccg gcggctcgag
     4261 gcgaaggatc ccgcgttgga ggctgtgggc atgtatcgca gcttccaggg aaccgatgtg
     4321 ggcagccgtg accggggtta tgtctatgtg ccgacgctgc agacgctgaa agaggtggat
     4381 gagtggtcgc atgtggagct gatgcggcgc gggcgctaca tctacaattc cggcggcggc
     4441 ctggtgcatc gcggcgtcaa cggggtggcg cgcatggtgt gcggcacggg gctcttccct
     4501 tatcctggct cgccaaacaa agcctggaac gccaaggttc gacggctgtg gatggcgcgg
     4561 tgcgagaaca aaaacacgtt cgatctctcg cggaaattca cctgcggctc tgcccagcag
     4621 ggcattgtgc gggccaagat caaagacggc gacatggcgc ctgtgctggc tcgtgacgag
     4681 agcggccggc tgcgctgcat gttttacgag gcgcatcaaa tcggccaggg cggcaaacgc
     4741 gaggcgcccg gcgagcgctg gcatcacggg gtgaggctgg gacggcataa tgagccgctg
     4801 gcgtatcgtg tcgtgagcgt ggatgatatt ggcggcgggc aaaccgaggt ggaagtgccg
     4861 gcgggcaatg tgctcttttg cgccacgtat gagcgcttcg gtcaggtgcg cggcttgagc
     4921 cgtttttacc ccgtcatcaa caaggtgctc gaccgtggcg agatcctgca cgcgctcacc
     4981 aagggcatca agatgcgcga gcagatcggc tttgccatcg agcagcagct cgccgtgcaa
     5041 atgatcggcg gccctggcgg ctggggagct ggcattcccc agcccacctc gcaagtggcg
     5101 ctgcccacag gggaaaagat cacgttggaa aagtttttcg gcgccggcga ggctcgcgaa
     5161 ctgaagccgg ggcagacctt caagcttgtc gaatccgacc accccaatga aaacgtccgc
     5221 gagcacctgg agacgctgac gagggatgtg gcctgggcgc tgggttacag tcccgagttg
     5281 ctctggaaca tcacccagct tggcggcgcg aacacgcgct tcatcatggc ggacgcgcag
     5341 agtcagatcg agagcgagca gcaggagctc gtcgagcagt tcctcgggcc ctggtatctg
     5401 gcatggctgc gtgacatgat tgaggcgggc gagctggagg atgtcgaaag ctgggagctc
     5461 catagctggg tgctgccgaa acggctgacc gtggacttcg gccgagacgg caagctgcac
     5521 atcgagcagg caaagcgcgg tcacatcacg ctgaaggcgt tgcacggctt ccagggggat
     5581 cagtgggagc aggaggtgga tcaatacctc gacgagcgcc tctacatcaa ggaggggatg
     5641 gccgcgcgcg gcttgacgtg ggccgaggcc ttccccgaga tcggcgccgg cacaaccgcc
     5701 gcgcaggagc agctcgacga cgatgaggcc gcgcgccagg cggaggaaac ccaggccgcg
     5761 ctgcaacggg cggtgaacaa accgccgatt cgaatctaaa actttcccag cgcggaattc
     5821 cgcgctggga aaaaagtgaa cattatgcac tctgaaactc tccatctccc gcaccttgcc
     5881 cggcagtttt actttgagcc gctggcgctc gaagaatccg ccatgctggc ctgtcacatg
     5941 tatctgtggc cgcgcatcac gggtcaggcg gcggatttgt cgcctctctc ggaggccaag
     6001 gaaagcaacc ctggcagcat caaatacggc ggcgggctcg cccacatgcg caagcgtatg
     6061 gccgagccga acatgcaaag caacgggccg tttgccgcgc cgacgattct tgatccggcg
     6121 tattattgga ccgtggaagg caagccctcg atggcggtga ttcccatcaa tggcatgatc
     6181 agcaagggca tctcgccctt catggaattc tgcatgggcg cggtgaatcc cgaccgcatc
     6241 agccatgcgt tgcgccaggc ggtggaggcc aagggcatca agcaaatcgt gctcgacatc
     6301 ggctcgcctg gcgggcgcac caccttcacg cccgagctgg cggcgcaggt gaaggcggct
     6361 gccgccacgc ggggcaagac cgtgtatgct ttcacggaca ccatgatcgc ctccgccgcc
     6421 gagtggctgg cgagccaggc ggatgaggtg atcatgacgg cctcggcgca ggtgggcagc
     6481 atcggcacgt atcttgcctt cctgaatccc aaggtggcga tgcagatgca gggctatagc
     6541 ctcgagctgt tcagcaaggg gacgcacaag ggcctggggc tgccggggcg ggatctgaca
     6601 caggcggatc gcgagtatct ccagggcacc gtggacagcc tcaacgccca gtttgtcagc
     6661 gcggtcaaag cgggccgtcc caaggccagc gaggaagcac tcacggcggc aaagatgtat
     6721 gacgggccgg atgcggtgcg ccacggcttg gcggacggca tcgtggggag ctgggatgag
     6781 tttgtggcgc tggtttgaca cagcgcgcag ggcgtcctcg aaacgacgcc atgaaacaca
     6841 ccctctcctc cattcgccgc ggccgctggc tgcatgtggt ctccctgctc attctcgccg
     6901 tgctgtgcgc gccgctgctg ctggcggtgc ctgctgagcc tggccttgcc ctgtgcggta
     6961 tccccgccct gatgctgccg ctgcgcacgc cagcggatga cagcggcgaa ggtggcggcg
     7021 gcggccctgt caatgtggcc gatgcccttg ccaagctcga agacaagacg ctcccgctga
     7081 gtcagaggct gagtgtcgcg gcagcagcgc tgcgcgggat tgatccgacg caacaactcg
     7141 ccggcacaaa gctaaagctc gacgaggcct cggcagaagt ggcgcgcctg aaaagcgaac
     7201 tggacggctc gcggatgcaa ctcgccgcgc gggagaagga cgtggcggat cttgaggcgc
     7261 gtgtggctga gttggagaaa gcccatgcgg atctggcggc caaggagcag gaccttgaga
     7321 agcgagctgc agccaaggct caggagcgca tggccagcct gggcttcccc gccggcaagc
     7381 tgcctgcgcc gagcgcggat gactcggggc tgaaggacgt gccgaaaacg gaagcgcagc
     7441 tcacggaggc gctcgtcaag tgcaagacgc aaaaggaacg cagcgcgctg ttgcgcgaat
     7501 accgggcaag cgtccatgcc gcctgatttg acacttcgtc tttcacgatc ccgcaaacct
     7561 agccttccac tctcatgagc aataccatcg cctctgaact ccagctcacc acggtcctgg
     7621 actccgccct tgtggccttc cgccgcgcca tcctgcctct ccagcaattt gccacctctt
     7681 acagcaacgt gccgctgcgg ggggacaaca cgatggcaat tccttactat cctctggcca
     7741 ccagcgacag tgtgacgcgt gcgagcgggg gcagctacaa ggcgctggcc acctccacca
     7801 cgacgcaggc caagaaggtc gtgatcaaca agaacaaggt gcaggccatc agcttcacga
     7861 gtgaggagcg ctcccgccag ccgatgttca accccgagat gcatggccgc ctgaaaggcg
     7921 aaaagctggc cttcgacatc attgccgata tcttctctcc tgtgcgccac agcgtcttta
     7981 ccggcgccac catcagtgcc accacggcgg gcaatttcga tgaaaacgac gtcgccgact
     8041 tggcgcagaa atgcatggag gactactggc ccgaagtggg ccgcagcctc atcttgaacc
     8101 cggccttcca tttcaacctg ctgaagcagg ggcagatcat tgacgcctcg aagagcgcgg
     8161 atcctctggc gttccgtgag gccaccattc gccgcattct tgggtttgag gagcttggca
     8221 ctgccggcct gccgaccaac aacggcaccg ctttcgcggt cctgggggag gctgatgatg
     8281 acaccttcac ggcggcggcg cacggtttgt tggatggtga cagggtgatt tttccgactc
     8341 tgacgggcgg cacgggtctc acggcggcga ccacggctta tttcgtgcgt gatgccgcca
     8401 ccaacacgtt taaggtgtcc gccaccatcg gcggcgcggc agtcaacttt acttcggacg
     8461 tgacagccgg cacctgccgt cgttatgaaa acatcgtcgg tgtggcggcg gtgccgagcg
     8521 ctctcctggt gggctttgct cccgtcgaac cgactccggc ggtgcgcagc cagcttttcg
     8581 actaccgcgt cgtcgaagat cgttactccg ggattgtgct tgaatacaag cacatcggat
     8641 acggtgacac agacgaggaa gtccaagtca tcgaggcaca ctacggcgac gcgctcggag
     8701 aaaccgccgc catgaagctc atcaccacgc cgctttcctg agccggctga accctcttct
     8761 ttgatcgcgt atgaagctcg ccctcctcat cggcctggac gctgaaaagc gttcaggctt
     8821 tcctctcaca ccagtggcgg tgcctttcga cgaggctctg cgtgccttca agggcatggt
     8881 cgccgccggt gtcgcacccg cgccgcatgt gcagctctgg tcgagcaacg gcctggtcaa
     8941 gcaggcgcgc ttcaaaaaag tgcaagcgcc aaaggcggcg aagggttcga aaaccgagct
     9001 gctaaagcgc atctccgaac tggaggcgcg cgtcgtcgaa ttggaggcac agaccccaac
     9061 tggcgcggca caaactccgc cgctgaccag cttggaaaaa gaaccggagc cttccttgga
     9121 agaccccgcg ccggacagcc ctctcgttcc aaagacctga atttcctcaa gcggttcctc
     9181 cccgtttgtt ggtgtgactg aagcccgctg gttgggttgg ccggcgggct tctttgcgtt
     9241 ttgacatcac caccgggata tcatgaagct ggaggaactg acccaagacc tggaccggaa
     9301 aaacggccgc cgcgtgatcc gtgtcacgga cgctggcggt gatgagcgtg tcacgatgac
     9361 gctcgaattt gaaagcaaca ccagcctgcc ggatgaggct ccgagatatg ggccggtgga
     9421 tcatccgccc gccaacagca aggtgcgcga gtatgctctc gaactgctcg ccgcgctgca
     9481 caagggggtc tggcgtctgg atctgacggg agggcctggc gctgcgtgaa tgccaggcag
     9541 gcggcttttc agaaggcgat gcagcgcggg cgcgcggcat cgccttttac gggcgaggtg
     9601 acggtgatca tgggcgcgac ccggcatcgt gtgtgcgggc tggtgagcag cggtgacaac
     9661 aaggctcagg tggatgagtt tgggctggag catgtgcgca cactggaggc tcatttgccc
     9721 aaatgccggc tgcctcggcg gccagacgcg tcgctggatg cggtggagta tttggggcga
     9781 agttacaagc tcgcgagcat caaggggggt gatgcctgtt ccgatgtgtg ggtgatttcg
     9841 gcggtagcgc ctttctgagt tatggacaac acgcctgatt ttttcatcgc gaaactgctc
     9901 gtggattttt tgaccgccac gcaggccggc acaccggacg tggagattcc gaaggtggcg
     9961 ttcgacggcg gctcggtgcc gacgccgttg tgcatcgtcg tggtggccag ggaggcgcag
    10021 gaaaaaaaga ccacggtccg gcgcatggaa attacggtca acctcatcac ctggctgaag
    10081 gcggcggatg cgggcgcggc ctcggtggac actcagacaa cgcgcgagga tgccggtgag
    10141 atcgtgcgcg ggctggagga tcgcctgcgc gatgatgctg cgtggaatac gttcattgcg
    10201 ggcgttgcgg cgggagagaa tgccgcgcgg ctccagggtt ggagtattgt ttgccggcgt
    10261 tttgagggcg tgggttcgcc gatgcgcgag gcggaaacgc ggaagctcac ttacccgatc
    10321 aagctccgtt attcgctcgc cgtgtcgcgc ggcgtttgac atcgcgcgca tggcgaagct
    10381 cgaaaaataa tcccgccatg aaacatctcc tctctgctgc tctcctcgct ctcgccctcc
    10441 tgctgcccac ggcggctctc tgtgccgatg tctccgtgac ggccggtaat gtgcgcacgt
    10501 cggactatgc ggtcatcgct cccggcgttg cgggtgagac gatcaccgct ggtcaggtgc
    10561 ttttcctttc cagcgccgat gaccgatatt acctggcgga cgtgaacgcg gcgggtgaga
    10621 cattgatcgc cggtattgct gtgagtggcg cggcggcggg gcagaggctt ttggtttgca
    10681 ttgaggatcc cgactttact ccgggcttca cgctttccac ttcggctccg gtgtatgtgg
    10741 cctcggcgac tgcgggcggg atcgctccgg tggcggatgt taccacgggc tggtattgca
    10801 ccgtcatcat ggttgccaag tccaccacga aggcggcatt tcgcgcgcga ggtctggcct
    10861 cgggcagtgc cgccacagcc ccctgatgct ccttttggct cggtaaccca ttcacttctt
    10921 ttcgttatgc cctttgatcc tgtccaagtt tacggcgccc tgccggcgta tgatcgccgc
    10981 gagttcgctg agtcgggttt tcttaccgaa accgtgaaat tcactcctga gcaagaggtc
    11041 aagcaaaaga aggctcacgc cgtcgcggct ctcggccagg tggcgcaggt gcagatctgg
    11101 cgcggctcgc tcgccatcga gatcgctggc gcgctggtgg cggatgcaaa tggcctggtg
    11161 catggcctgc cagccggcta tgtcggccaa gctgtgacga cctgcgcgca tttcgccgcc
    11221 ggcaccgccg aggtgcccgc catcgcgcgg cacggctaca cgcgcgatcc tgagaagctg
    11281 ctcctgatcg agaccgcctc cactgacctc ggcgaggaag aacctgccgc cactctgggc
    11341 ctgcgttact ttccgtttgt ggacgccaca ccgctcctgc ccgcctgatc cctacccata
    11401 caaaacatga gcggcgcgcc gggcgtcgcg gtcatgagat gctgacgagc ctctcctgcc
    11461 gaagccgaaa ggcgcccggc gcgctttctt acccgaacct tccccgaact tatggcactc
    11521 actccgcagc gcgccgcaaa tacgctggcg attactgaaa cccggctggc cgccagtctg
    11581 cgcgcgctgg gcttccccta caccgccgag acaacgattc acgagcgcac tggcgctgcg
    11641 gtgattcagt ttctttttgg cctgtcgagt ctccgatttc cggccttgaa actgccgcag
    11701 atgctccagg cctggaggtc aggcgagatg gctcgggagg atccgctgca cgtgctgcat
    11761 gtcatgatga cggcgcatga atgttacgac gcgctgctgc gcatgatgaa agaccatgtc
    11821 agtcactgcc tgcatctgcg cagcggtggc gtgttgacgg tctatcggcc tggcgcggct
    11881 gcaccgcccc ctggcgctcg gttggtgtgg acgaatgatc tcaatctggc ggcggcctgc
    11941 ggcgtggtcg gggtgccggt gctgagcatt gacggcagcg cggggcagca tcgttttgga
    12001 ctcgctccct gcggataccg agtgctgctc tcggacggtg gctggcaggc acatgacacc
    12061 gccaccctca ttgcccgcga cccggtgcgg gaggacgaat ggaggctgcg gatggaagcc
    12121 acgcagccgc tgcatcccct gtgcctggcc tacgatgcgt tgcacaatcg cgcgcagctt
    12181 aaggccgaac tcaagggcca ggggcgcgcg ccgatgatcc tcattgacga gcctgcgcgt
    12241 gtgatccccg gcgcgcacaa gggcgcgtgc ctggcggtgc ggcaggctct ggtgagcgcg
    12301 gacgcgccgg gccatgtgat ggatcaggtg acccgccata tgcaggcccc accgatgtcg
    12361 tggtgacatt tttttccgaa cccgaacccc atgaaacgcc gaacccaatc cacctctgcc
    12421 gccctgcctg accctcctca tgagagtgag tcgccgggca tctccgcgcc cgcctctccg
    12481 ccgcctgccc gcaccggtgc cgatgatgac agcaaggcgc gcgcggccgc cgcgcagcgc
    12541 gagcagctct gggatgagga gggcacgcgc agctttgccg gactgcctct gctgccctgg
    12601 agtcgagagc gccacactct ggcggcgcgg ctggtggcgt tggatctgcc cgctccggcg
    12661 ttggccgagc tggtgcgcat ccgcgagatg ctggatgcgc gcaaggacgc tccgttcgtg
    12721 cctctgtcgt tcgcggtgga cctggctgcc tatctgccca cggcggaaaa gatcctctgg
    12781 ctggccactc acgaggcgga tgagatcaac gccctgcgca cccggccaac gctgctgctg
    12841 cagacggtgt ctgagtggtc ggtcagcgtg atctcgccgg agctttttga gccggcgtgt
    12901 ttgctggcgc atcgtctcac cagtgagcat ctgcgggtgc agccgatgcc aatgccgaac
    12961 aaaaaaggga agggtcgtga ctcgggaaac tagccttgcc cgccacggga gccgagtatt
    13021 acgcgctact gtcgcgggca ttgcccggca ctccgctgca cgttctgcgc tggcaggtga
    13081 gtctccacga ggcccgcgcg ctgatccatg ccagcggcat cctggagggt ggcagctaca
    13141 tctggcccga ccccgagctg agcagccgcg gtcgcgccat gctgcgcgtg cgcgaaaccc
    13201 tgcgccgcgt gcgcgcggga gagtgggagg tggatttgtg agggcgaacg cccttgcggg
    13261 cttttgccag cgcacgtaaa gacggctggc cgcgctgccc cgagtgtggg aagcgactcc
    13321 ggccctgcga aggaaacatg cgtgaaacag gaacttcacc ccctcaacaa tggcactgca
    13381 tcccagctgg tgggcgaact caatagcccg aacatccaaa ctcacacacc gcatgagcgc
    13441 ccctgactcc gcttccatct ccgacacaac cgctcatgcg gttgtggtgc agtgcccttg
    13501 ttcttggtgg gtcgttggct ggcatcgctt tgccgcccgc cgtctcttgg atgacacatt
    13561 accggctact gacgcggcga ccataaaagc attgatgcgc caccggaaag ccatttgccg
    13621 cctcgatcag acgaacgacg aagctctgcg atccgcgccg acagcggacg tggggcgcag
    13681 aaaggaatct ggaatatgag caagactttg aacgcagagt ggggcgcgga ttcgcagcag
    13741 cgctttgtta gacgaccagg cagaaacata accgtctctg gtgtgcgctg gcgctggcga
    13801 atcgggaaag gtggcggatg cgtggcatac tccgaaacag gagaacgccg atgcgagcag
    13861 gcgtggcgca tcaaaggcca aaaccacccg gacattttcg gtcgcgggca atggaagaaa
    13921 acgagtgacg gcatgttgct accctcagag gtcgcggcgt ggctgtcgtc taacactgaa
    13981 tacaccaacc cggagggctg ccaatgacga ccgcggacac gaaagactcc acccagcccg
    14041 cagggttggg tgccatgact ggttcggctg tttacgtgtc gtttcgggaa atgcttcgcc
    14101 gtgggtttcg atgcgggacg ccagagccca gcagtgatgt tgcaaagaaa cccaaaaagc
    14161 tggggaaatt ccgtcgcaag aaaggctccc gtgtgtgggg attttcgtca tggaattggg
    14221 aggcagatca cggtgattac atggtgcgac acttggatgg aacgctttgg cgactggagg
    14281 aatccagggg acgaaacaaa gcggcagcag gcgaactaac tccacgcaag attccccctc
    14341 ccgttgctgg agctattgcg gagattcgcg gaaatgacgt gttttgggtc atgccgaacg
    14401 atcaaactgt gccgaccgct ggggcgctcg gcgcatccga ataaacccaa gaccaaccgc
    14461 cccagcggtt ggcaccagtg ccgtgttatg ccactagacg acgccgaaac cgtaaaccgc
    14521 atctctcaaa tcctctttgg atacagggag gcgcccgagt ccgaaatctt cacgcgactg
    14581 gagtatctgg tcgagcgcga aaagtcgata aacctgcacg acgccgtaaa aggcggaaag
    14641 ttgaaaatcg ccatcgaggc gctgcatgag gcgctggagg gattggcata acagttgaat
    14701 taaccccacc agcattctga attaatatga acaccttaga attcgtcgaa caccgagctg
    14761 atcaaccgcg ccccaggcgc ggtttgatcc tgcggtgggc ccggcggcgg tttgacaccg
    14821 cccgcccatt aagccgaacc catgtctctc gtcatccact ccgaagctca cagcctagcc
    14881 tttgccaggc tggtgagcac ttcaaaaaaa gctgcggtgg atttggttcg gcaggagttt
    14941 gggctgctgc tcacagaggt ggcaaaagtc acgccgccct actccgagaa gatgagtgga
    15001 aaaaaggcgg aggttcaagg ccgcgcagcc gtggcggcgg acatccgggc cctttacggc
    15061 acgccggggg atgcgtatga cgccatctct gcgccggcgg ccaaggcggc gttttggttt
    15121 ttgcaccagt caggggacaa tgcggcggcg tcgcagattc tgcgcgcgga gactggcacg
    15181 ggcctctccc ctttcgatgg cggcacggtg catggccggc ggcgtccggg taatcgccgg
    15241 cggcggcaac ggcgcgtggt ctattacgtg gcggacaccg atgcgctcga tgtctacatt
    15301 gcggccgagc aggcgcatgt gtggtggctg gcctccggct gggcaccggc gctgcgcgct
    15361 ctggggcggc gtctgccgta tggcgtggag agacattccg cacccggcac gatgcgcgcg
    15421 gtgatcaccg accagcgcat tgagctggtg gccgtggaca gcgtggcttt cgcgtcccgt
    15481 gttcgggaca ttgagcggca gatccagttt gccctgaaga tccgcaccgg tgccatgcag
    15541 cgcagttggg atcattttac ccgcaatgtc agactctgag ttagaccatg gccgccattg
    15601 aagcatctct ccggctcgaa atcgcccagt atcaggcggc gatggctcgc gcgcgcggag
    15661 agattgaccg gctgaaggcc cgcgccaaaa gcggcggcgc gggcttgtcg aaggatttgt
    15721 tcggcggcgt catgcgcggc gcggcggcgc tgggtgtcac cctgggcacg gcgcggattg
    15781 ctagcggtat ctttgactcg gtcgtggcca tggagtcgct ggaaaaaatg ctccgcacca
    15841 ccgagggcag cgccgaggcc gccacacaac gcatggctga gctgcgcgag gtggcgcggc
    15901 tgccgggcgt gggctttgag caggcggtgc gcgcggatgt gcgcctgcgc tccatcggct
    15961 tgacggcgga tgacagcaaa aacgccatca ttgaatttgg caacgcgctg gcgctcgtcg
    16021 gaggcacgcg ccaggacttg gatggcgtgg tgctcgcgct cgggcagatc agcgccaagg
    16081 gcaaggtcag cgccgaggag atcaaccaaa tcgccgagcg catcccccag gtgcgcgccg
    16141 tcatgcgggc tgtcttcggc accgccgaca cggaggcgat ccaggccatg aagctggagg
    16201 ccaatgattt catcagattg ctggtcgctg gcttagccga gctgcagcgc gcgcaaaagg
    16261 gcctgcagga tgagctggcg gacatctcga ccatgggcat ggagatggcc aatgacatcg
    16321 gcggcgagct ggtgcgcacc ctgatccccg cgttccagga cgtgctctct gtcattgccg
    16381 ccaatcgcga agggctgcaa tccttcggtg ccgtggcggc ggacgtgttt tcagcggtgg
    16441 ccgacatcct ggaggcgacc agcatcatag ggtatgccgg cgcggcctcg atcatcggtg
    16501 gcgaaaactt tttcgacgct catcgtgcgc gggctgagga ggtgcggcgc gccaatgaga
    16561 gggccgcgcc cgcgcccgca gccag
//
LOCUS       DAOMYJ010000110        16412 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Prosthecobacter sp. isolate AMR_MDS_5629
            NODE_1223_length_16412_cov_19.404169, whole genome shotgun
            sequence.
ACCESSION   DAOMYJ010000110 DAOMYJ010000000
VERSION     DAOMYJ010000110.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867850
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prosthecobacter sp. (wastewater metagenome)
  ORGANISM  Prosthecobacter sp.
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Verrucomicrobiaceae; Prosthecobacter.
REFERENCE   1  (bases 1 to 16412)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16412)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.30
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:34:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,742
            CDSs (total)                      :: 4,684
            Genes (coding)                    :: 4,676
            CDSs (with protein)               :: 4,676
            Genes (RNA)                       :: 58
            tRNAs                             :: 55
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16412
                     /organism="Prosthecobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1223_length_16412_cov_19.404169"
                     /isolate="AMR_MDS_5629"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1965333"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..417)
                     /locus_tag="PLP58_12435"
     CDS             complement(<1..417)
                     /locus_tag="PLP58_12435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ09375.1"
                     /translation="MHRLLAALSILTCLPCPALRAQQLDLEQIFEDRNLGPVNELLGT
                     GEYELCSRVCEAAIQRGMKSPQWRVMRLKALMLLGRETEARDEAQLAVKTFPGDLELL
                     MLQHDNALRLGRRDIADEALKAVNDAARAKPAKDRAA"
     gene            547..3363
                     /locus_tag="PLP58_12440"
     CDS             547..3363
                     /locus_tag="PLP58_12440"
                     /inference="COORDINATES: protein motif:HMM:NF017044.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HRJ09376.1"
                     /translation="MPRPHALLCLLSLSLLTPVLLRGQWPQDGSDLKADPKAVFGTLD
                     NGLRYIVLPNDEPPGRASLRIYMDAGSLMEEDDQQGMAHFLEHMAFNGSRNFPGGEMV
                     EYFQRLGMAFGADTNAHTSFKETVYMLELPKVEPKLVTEGLQLFRDYLDGMNLDAAEI
                     DRERGIILSEKLSRDSIEYRTMIAGYKFALPESLLPERLPIGIEETLKTMQRPRFADF
                     YEKWYTPKRAMIVAVGDFKDVPAVEAEIQKQFADAKPRRGDAPDPQLGKISTGRGLIA
                     KLHTEMQAKSVDISIENPRPAKNKPDSAAARREKMVRDLADAMLNQRFSKLVKAEGSP
                     ILAGESYSYEYLEFVEVIGVMAQCAPENWQAALTLLETELRRAVEHGFTDAEFDEAKA
                     GLLKNAQLRAAQADTRKSRDLASGIVSVMAAKKVFTHPSADLARVKAAAESLTREECH
                     RAFVETWSSPDRQLFVGGNLKLEGDAEAQILATYKASQEKPVEAPAQEQTAAFAYADP
                     GPPGKVASEKVDAGLEITQAVLANNVRVNIKPTPFEKGSVRVTLNFGGGKLTAPLDKP
                     GLIPFANSTFIAGGLEAHSADDLRRLFASRTVSTDFSVGDEAFLLAGRTTADDLEAQL
                     QLLAAYLKAPGYREEAQRQFQNNLEPMYVQLRHTAEGVMNDKVVAFIHGGDPRFTFPD
                     IEEMRKRSLAELKAWLTPALTRDWLEVSITGDVEAKAALDAVTRTLGALPARSGEKPA
                     YEKERQIAFPKGPQTRDFPFQTEIPKAISILYWQTGDMRDIQRTRRLSMLASILDDRL
                     RLKVREELGETYSPACYHVANDTFTGYGYMTAMIECKPEQAAALAKLVTEIAAGVAAG
                     PITPDEFERAQKPLMTQIEQMRRDNRYWSQNVVRNCQEHPERLDWASSMVSDFGSVTL
                     EELQKLAATYLGAGRAVAVKILPQ"
     gene            3437..3844
                     /locus_tag="PLP58_12445"
     CDS             3437..3844
                     /locus_tag="PLP58_12445"
                     /inference="COORDINATES: protein motif:HMM:NF019013.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030638 - polyketide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ester cyclase"
                     /protein_id="HRJ09377.1"
                     /translation="MPHSSAALIEAATAALFERGELDAVETYFSPDYLVHLTGGEQVP
                     GHAGLRSVLALYKRAFSGLRAEVVILAAEGGRVAWQRTFRAVHEAAFKGFPASGRELV
                     WRDMGASLIRGGVIVEEWVLTDLAERLLLARKS"
     assembly_gap    4299..4398
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <4399..4633
                     /locus_tag="PLP58_12450"
     CDS             <4399..4633
                     /locus_tag="PLP58_12450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ09378.1"
                     /translation="PKSDWHFPKSGPHFPKSGPHLPKSGWHFPKSDWHFPKSGPHFPK
                     SGSHFPKSGWHFPKSETHLSVRNEKAPSRDLLP"
     gene            complement(4773..5378)
                     /locus_tag="PLP58_12455"
     CDS             complement(4773..5378)
                     /locus_tag="PLP58_12455"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: protein motif:HMM:NF013670.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HRJ09379.1"
                     /translation="MTAALPRRLWLLLLWLACAGAAQALSFSTVVLDPGHGGRDGGAV
                     WNKLVEKNLCLDVAKRVETLLKKKGLKVVMTRRTDTFVELGDRAWIANRQPRSIFVSI
                     HFNASRNTAIRGVEVFYRSTNGRALASRILTALRARLKTTSGRGLVHGDLKVLRATTM
                     PAVVVEAAYISNKAEAARCATAAHRQAIAEAIAAGILAARG"
     gene            complement(5446..6288)
                     /locus_tag="PLP58_12460"
     CDS             complement(5446..6288)
                     /locus_tag="PLP58_12460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ09380.1"
                     /translation="MSTTPKLLVELAGASGVGKTTAAPILARRLREILGMENVAALPE
                     KDLPRRQRRWRHMQRRLWILSHPADVLAAWRASRARPVIARFSVWLDLFSTHGIAWRA
                     LGGEVRTALIDQGFLRLSMTPAHAPFLPGHLLPGLVIQLVADPAVLEVRRITRDKVRH
                     KLYEGPQRLANARRSRQLMSTLPEAELRAMLGQFGGKFCNPPLSSEEIGQILRSSAGI
                     PDAPATQGFIRCHPAVLAALQARGVRVVQVDNSRQDGIEPVIEQCLQIILAHSHAPAQ
                     GAPS"
     gene            complement(6418..8661)
                     /locus_tag="PLP58_12465"
     CDS             complement(6418..8661)
                     /locus_tag="PLP58_12465"
                     /inference="COORDINATES: protein motif:HMM:NF019219.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1549 domain-containing protein"
                     /protein_id="HRJ09381.1"
                     /translation="MKNRLLLPLLALLLGAAAQAMSPDDLRAKSAEIDRLILAKLEKE
                     NLQPNAPASDEVFVRRIHLDIAGRIPTLKETTDFLADSDPAKREKLIDRLLASDGYQQ
                     HFFNFWSDLLRLKSQSVGGGQSLPAGYAYADWLRESLKTNKPYDQMVRELVSADGKTY
                     ENGAVGFYIRDYNMPLDNMAVTTQIFLGTSMVCAQCHNHPFDKWSQMDYYQMAAHTYG
                     MTGSNGLSNPLLADAFYGRSGKKNKEMMAGAGKKDMGKAMTEILRPLRYNTVLDQTDK
                     RTLRLPHDYQYSDAKPKEAIEPVIPASFSKDGKITREGMKPVEAYAQWMASKDNPRFT
                     LVIANRLWKKAMGMGLIEPVDEITDSTVPSNPQLMAFLEQTMKDVNYDMKAYLRVVFN
                     SASYQRAAHTQDVQLGEQYHFPGPLLRRMSAEQIWDSIVTLHKPGADVPSADARVERT
                     VAIRRIEWLDRALNALSSQELAAGAAKIAALQKQLAAEVRAAQEKLVKAQENNDEKAI
                     REARRVVSTQRSRINEAAQDIVYTMGFKKFAELAREGKLKEQVDDVDFAREITALIKS
                     RQDEGLTIDDALNIVARQQKARLNEQQKARLQRDAETFNAATKQDKSQLLSWERMRDT
                     VMVRAADLRSPAPNGHFLRTFGQSDRELVDNGNQEASVTQALALLNGGTFNDLLNPYT
                     VISRALERAKTPDAAADTIFLSLFSRKATDEEKGLLAPVIEGDAEKGRGDALWVALNT
                     RQFYFIE"
     gene            8864..9928
                     /locus_tag="PLP58_12470"
     CDS             8864..9928
                     /locus_tag="PLP58_12470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ09382.1"
                     /translation="MRTSARFFLLALVFANVSAQACSVPVFRYALEHWPADPFQVLVF
                     HHGPLSGGDQAALREAEKREGARPNVIFRTVDLNDEAPGDLRRLFESQQGARTPWMVA
                     RFPSSSGIPVPVVSQAFTPGALKVLLDSPVRAEVVKRISSGESAVWLLLESGDKAADD
                     AAEKLLQERLDYLASVMSLPKLDDSDIANGLVSVAEEDLRLDFSLLRIRRDDAAETAL
                     VRMLLLSEEGLEELDEPMVFPVFGRGRALYALAGAGIRQEMIESAAAFLVGKCSCQVK
                     EQNPGVDLLMTADWEAAAKASPVLDRELPTLRELVPAQPESSTIEAVADGGSFPAWRA
                     WSLGMGVLVLAGIGFRLLRK"
     gene            9988..10908
                     /locus_tag="PLP58_12475"
     CDS             9988..10908
                     /locus_tag="PLP58_12475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009963677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HRJ09383.1"
                     /translation="MNQARISSRRHFLMQSGLVAAASVLPAARRAAASPDIPHALAGR
                     LYKTLKIGMVKTKGTLTERFKTAKAAGFMGIEMGFPGDDVRDTKAAIAASGLPVDGSV
                     CGQHWQVRHTSPDPEVRARALENLKQALRDTREVGGHSVLLVVGKGEDGPENEIWPRS
                     VENIAKAIPLAAELGVQIVIENVWNQFLYNHEGGADQTADKFVKYVDEFRSPWVGMQF
                     DIGNHWKYGSMGDWIRQLGHRVYKLDVKGFSRAEGKFTPIGEGDIDYADVRKALHEIN
                     FHGWLAAEVAGGDLEHLTGVARQMDAAFGL"
     gene            complement(10940..12646)
                     /locus_tag="PLP58_12480"
     CDS             complement(10940..12646)
                     /locus_tag="PLP58_12480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ09384.1"
                     /translation="MKNHPASFARLETLEARLAPAGIVALNLSGSLLTITGDAFGNEI
                     GISEAGGMWTVEALPGSATEFSLNRGPLLSSVTFAAPASIRANLGAGDDVLLLSGLTM
                     SGYLTVNAGDGSDTLDLTSTFINGAVTAGMGNGDDVFTAGGDLFFGRGLNVNLGAGAD
                     TFELNATSLLANAAITAKGAGTPVDLQSFTLAAADGLVKGAVTLSATGNAPADFIIGD
                     LPDDLLTVTGALNLSAGAGEDHVFLSGTLDIAGMLNIRLGNGVNLVRSDDLGDLFARG
                     LFYGGGSGTDELILLGRDLDLATTLTFNGGAGTNRLELDQTGFTTIGGALTYNGGAGV
                     DVLLIGGADTLVGGLVAMNAGAGENAFGLNSVLASVGSVRFTGGAGNDVVDIGENTGA
                     SDLVTVRGAVNVNTGAGSADVLVRDADIHGALNITTNSPFGGIDLVRILDSDVRGAMM
                     TRMNGGADSDVIVRDSIFDRNATIHTGNGDDLVEFDTDTDVSSIFSVFHGYVRVYLGA
                     GNDIFLAGSNPAVNTVGNDFRGYVDVHGGAGYDRVYFMDPAYNNIFPGGEPLAFTTEE
                     VY"
     gene            complement(12709..15372)
                     /locus_tag="PLP58_12485"
     CDS             complement(12709..15372)
                     /locus_tag="PLP58_12485"
                     /inference="COORDINATES: protein motif:HMM:NF024893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA-like C-terminal region-containing protein"
                     /protein_id="HRJ09385.1"
                     /translation="MNEFLRRVIRLAAASALLAVIVYAGTDAFGRQWRDFVTGQLAER
                     GLHMDFRRLVLDPFGGLMARDVRLYSRSDRQELLVSVDRLRMDVEFGRLVERRFEVQG
                     FELSKARLSIPVKGQDEDGADTMIEVEDLSARAFLRERRLELRHAEGTISGIRLRIAG
                     EMLLPSKDEDKKPEEKTSFSVEERIKVLRNHQQRIQKGLDWLRRFQFASPPQLSLDLR
                     GEMDRLAEMNLRVVFEADDLSYGAYTCRELVAEAEYRAGVVDLTRLFLKDPTGLVNAN
                     ANWRMGSPEVRFHLNSSADLPALALAFLDNDNLREVVFYESPNLALDGVWHVLGDLSA
                     HKRPVRLNGSLDCGRFSTRGAVFDGLSAKFGVAPEGVYIRDIVLRHRTGTLSAQALSH
                     EEQGFRYRAVLRMDPNAFIPFAQMEKTREIIRRFEFNPASNIFFELEGAGPKPDLQQC
                     LNSGRGELRNFKYRGVEMELLTADVEFKHPVQHYRNVRLRRTEGAAEAAHVQVDDEEK
                     WVRLTGVKSGVDPVAVTSCFAPKTAANIAKYRLPEDTRVELDGVIFHKDGMRSDFQVR
                     FEHPTGTGKYSVLGEEHTIHSPEGRLHIKGGVLNFDINGRVHGGAMNAKGGVWIIEGR
                     DDFNVAVKAARFPYRVFDKPAPFEKVTADVSGKGGTIRFDTRAALLGGSFSIKGTLDE
                     SRDPMPYTGEMRLENVSLQRLSQLYSKDSATQGDVTGHFRFEGRQDDWKALKGEGAAI
                     ILNGNLYSVPILGPLTPLLDSVLPGKIKDYNVATEANCTFQVAGGLVTTSNFEALTNL
                     FKLASSGSINFIEDKVDLTAQVRVRGLPGIVFRPFSELLEYRGEGSIKNTQWSPSILK
                     GTQRETESSPKAAPSRRLTLPLFNRGEAGKP"
     gene            complement(15410..>16412)
                     /locus_tag="PLP58_12490"
     CDS             complement(15410..>16412)
                     /locus_tag="PLP58_12490"
                     /inference="COORDINATES: protein motif:HMM:NF036541.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DR2241 family protein"
                     /protein_id="HRJ09386.1"
                     /translation="VVDAYMEEPPLVSDWAELTRAKHVVVVPFFIADGLHSFQDIPVL
                     LGMESEPGKALSEMDVFRQNPIEMRGRKLYYSGAIGTEPLMTDVILDQVRDFDLRHGI
                     ADVNTEPPTGTGITAALESWLGEGRNVIGQLRITAAADGYDLRHVEDGEAVELRTCTE
                     PEAAREWARLDAEGAFRPIKTAPNLRRGWRLLLPDVEAVRIALDCLYPAAPGMALAWQ
                     EGRLRPGPLRVLLGRQTGMYRYANSITDEQAGEIIGRCCETRTRCLRRIVFPMDDSQS
                     LPGPAMQKLGGAAGRAPGVDEARAIPLLCMEACSHIVSAARKQARENHQKQEQGGGD"
BASE COUNT         2795 a         5295 c         5282 g         2940 t
ORIGIN      
        1 ggcggcgcgg tccttggcgg gtttggcgcg cgcggcgtcg ttgacggcct tgagcgcctc
       61 gtcagcgatg tcgcgcctgc ccaggcgcag ggcgttgtca tgctggagca tgagcagctc
      121 caggtctccg gggaaggtct tcaccgcgag ctgcgcctcg tcccgcgcct cggtctcgcg
      181 ccccagcagc atgagcgctt tcaggcgcat gacacgccat tggggcgact tcatgccgcg
      241 ctggatggcg gcctcgcaga cgcgcgagca gagctcgtat tcgcccgtgc cgagcagctc
      301 gttcaccggc cccagattgc gatcctcgaa gatctgctcc aggtccagtt gctgcgcgcg
      361 cagggcgggg caggggaggc aggtcaggat ggaaagcgcg gcgagcaggc ggtgcatgac
      421 acagagaacg cgcgccggga gcggattcga aaggggaaaa ttcctgtttg ccgcgcgccc
      481 cgccctgcgg cagatgcgcc cggcgcgcaa aaccgccgcg ccattcacgt tcgtatgcct
      541 tccaccatgc ctcgtccgca cgccctcctc tgcctcctct ccctcagcct tctcacaccc
      601 gtcctgctgc gcggccagtg gccgcaggac ggcagcgatc tcaaagccga tcccaaggct
      661 gtcttcggca cccttgacaa cggcctgcgc tacatcgtcc tgcccaatga cgagccgccc
      721 ggacgcgcca gcctgcgcat ttacatggat gccggctcgc tcatggagga ggacgaccag
      781 cagggcatgg cgcacttcct ggagcacatg gccttcaacg gctcgcgcaa tttccccggc
      841 ggcgagatgg tcgagtactt ccagcgtctc ggcatggcct tcggcgccga caccaacgcc
      901 cacacctcct tcaaagagac cgtctatatg ctcgagctgc ccaaggtgga gccgaagctc
      961 gtcacggagg gccttcagct cttccgcgac taccttgacg gcatgaacct cgacgccgcc
     1021 gagattgacc gcgagcgcgg catcatcctc agcgaaaaac tcagccgcga ctccatcgag
     1081 taccgcacca tgatcgccgg ctacaagttc gccctgcccg agtccctgct gcccgagcgc
     1141 ctgcccatcg gcatcgagga gaccctcaag accatgcagc gcccgcgctt cgcggacttc
     1201 tatgagaaat ggtacacccc gaagcgcgcg atgatcgtgg ccgtcggcga cttcaaagac
     1261 gtgcccgccg tcgaggccga gatccagaag cagttcgcag acgccaagcc ccgccgtggt
     1321 gacgcgcccg acccgcagct cggcaagatc agcaccggcc gcggcctcat cgccaagctc
     1381 cacaccgaga tgcaggccaa gtccgtggac atctccatcg agaacccgcg ccccgccaaa
     1441 aacaagcccg acagcgccgc cgcccgccgg gagaaaatgg tgcgcgacct cgccgacgcc
     1501 atgctcaacc agcgcttctc caagctcgtc aaagccgaag gctcccccat cctcgccggc
     1561 gagtcctaca gctacgagta tctcgaattc gtcgaggtca tcggcgtcat ggcgcagtgc
     1621 gcccccgaga actggcaggc cgccctcacc ctcctggaga ccgaactgcg ccgcgccgtc
     1681 gagcacggct tcaccgacgc cgagttcgac gaggcaaagg ccggcctgct caagaacgcg
     1741 cagctccgcg ccgcccaggc tgacacgcgc aagtcgcgtg acctcgccag cggcatcgtc
     1801 agtgtcatgg ccgccaaaaa ggtcttcaca catccctccg cggatctcgc gcgggtgaaa
     1861 gcggcggcgg agtcgctgac cagggaggag tgccaccgcg cctttgtcga gacctggagc
     1921 agcccggacc ggcagctctt tgtcggcggc aatctcaagc tcgaaggcga tgccgaagcg
     1981 cagatcctcg ccacttacaa ggccagccag gaaaagcctg tggaagcccc cgcgcaggag
     2041 cagaccgccg ccttcgccta tgccgatccc ggcccgcccg gcaaagtggc gagtgaaaaa
     2101 gtggacgccg ggctggagat cacccaggcc gtgctcgcca acaacgtccg cgtcaacatc
     2161 aagcccaccc cctttgagaa aggcagcgtc cgcgtcaccc tcaacttcgg cggcggcaag
     2221 ctcaccgccc ctctcgacaa gcccggactc atccccttcg ccaacagcac cttcatcgcc
     2281 ggcggcctcg aggcgcacag cgccgacgac ctccgccgcc tcttcgccag ccgcaccgtc
     2341 agcaccgact tcagcgtcgg cgacgaagcc ttcctgctcg ccggacgcac cacggcggac
     2401 gatctcgaag cccagctcca gctcctcgcc gcctacctca aagcgcccgg ctaccgcgag
     2461 gaagcccagc gccagttcca gaacaacctc gaacccatgt acgtccagct ccgccacacc
     2521 gccgaaggcg tcatgaacga caaagtcgtc gccttcatcc acggcggcga cccgcgtttc
     2581 accttccccg acattgagga aatgcggaag cgcagcctcg ccgagctcaa agcctggctc
     2641 acacccgctc tcacccgcga ctggctggag gtctccatca ccggcgatgt cgaggccaaa
     2701 gccgccctcg acgccgtcac ccgcaccctc ggcgccctgc ccgcgcgctc cggcgaaaag
     2761 cccgcttatg aaaaagagcg ccagatcgcc ttccccaaag gcccgcagac gcgggacttc
     2821 cccttccaga ccgagatccc caaagccatc tccatcctct actggcagac cggcgacatg
     2881 cgcgacatcc agcgcacccg ccgcctctcc atgctcgcct ccatcctcga cgaccgcctc
     2941 cgcctcaaag tccgcgagga gctcggcgag acctacagcc ccgcctgcta ccatgtggcc
     3001 aatgacacct tcacaggcta cggctacatg accgccatga tcgagtgcaa gcccgagcag
     3061 gccgccgccc tcgccaagct cgtcaccgag atcgccgccg gagtcgccgc cggccccatc
     3121 acccccgacg agttcgagcg cgcgcaaaag cccctcatga cccagatcga gcagatgcgc
     3181 cgcgacaacc gttactggtc ccaaaacgtc gtgcgcaact gccaggagca ccccgagcgt
     3241 ctcgactggg ccagcagcat ggtcagcgac ttcggctccg tcaccctcga ggagctgcaa
     3301 aagctcgccg ccacctacct cggcgccggc cgcgccgtgg ccgtcaaaat cctgcctcag
     3361 taaagcgggg caaccttccc ggttgcccga tgattcaaaa gcgcgccccg gacggtcgca
     3421 ccaccccgcg cccatcatgc ctcacagctc agccgccctc atcgaagccg ccactgcggc
     3481 gctgttcgag cgcggcgagt tggatgccgt ggagacgtat ttctccccgg actacctcgt
     3541 ccacctcact ggcggggagc aagtgcccgg ccatgccggc ttgcgctccg tgctcgcgct
     3601 ctacaagcgc gccttcagcg gcctgcgcgc ggaggtcgtc atcctcgcgg cggagggcgg
     3661 gcgtgtcgcc tggcagcgca ccttccgcgc cgtgcatgag gcagccttca aaggcttccc
     3721 cgccagcggg cgagagctgg tctggcggga catgggcgcc agcctcatcc gtggcggcgt
     3781 catcgtcgag gaatgggtgc tcaccgacct cgccgagcgc ctgctcctgg cccgcaaaag
     3841 ctgaccggcc gccgatggcg acggcattga ccgcactgac ggcattgatc ctgctggcat
     3901 tgaccctgct cccggcgcgc aaaagctgac cggccgccgg actccggcgc tgggaaccag
     3961 ccgcagccag cgcggtcctc cctccacccc gctggcacgg cagcccggag gtttgcgcca
     4021 tcttgcgcgc gcgctcatcc gccaggggcc gttgcccgcc cgccaccctc cctatgccgc
     4081 tggcccaggg agcggagccg ttcggcaaag ggactccgcc cacaaaggcc ttccttttcc
     4141 ggctggcact tccccaagtc cggctcgcac ttccccaagt ccggctcgca cttccccaag
     4201 tccggctcgc acttgcccaa gtccggcgcg cactttccca agtccggctc gcacttcccc
     4261 aagtccggct cgcacttgcc caagtccggc tggcacttnn nnnnnnnnnn nnnnnnnnnn
     4321 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4381 nnnnnnnnnn nnnnnnnncc ccaagtccga ctggcacttt cccaagtccg gcccgcactt
     4441 ccccaagtcc ggcccgcact tgcccaagtc cggctggcac ttccccaagt ccgactggca
     4501 ctttcccaag tccggcccgc acttccccaa gtccggctcg cacttcccca agtccggctg
     4561 gcatttcccc aagtctgaga cgcatttgtc cgtccgaaat gaaaaagcac cgtccaggga
     4621 cctgctcccg taacaagagg ccggggcact cgccgctatg acgcgcggca tccttgtgtg
     4681 gggatggttt cgaaaagaag ggggcgcatc ctccggcaat ggctcacgcc gctcatcccg
     4741 ggcggctgcg gagggcgcgg tgaggcgggt tttcatccgc gcgcggccag gatgccggcg
     4801 gcgatggctt cggcgatggc ctgcctgtgc gcggcggtgg cgcagcgggc ggcctcggct
     4861 ttgttgctga tgtaggccgc ctcgacgacg acggcgggca tggtggtggc gcgcaggact
     4921 ttgaggtcgc catgcacgag gccgcggccg ctggtcgtct tcaggcgcgc gcgcagggcg
     4981 gtgaggatgc gggaggcgag cgccctgccg ttcgtgctgc ggtaaaagac ctcgacgccg
     5041 cggatggcgg tgttgcggct ggcgttaaaa tggatgctga cgaagatgga gcgcggctgg
     5101 cggttggcga tccaggcgcg gtcgccgagt tcgacgaagg tgtcggtcct gcgcgtcatg
     5161 acgactttga gtcctttctt tttgagcagg gtttcgacgc gtttggcgac atccaggcag
     5221 aggtttttct ccacgagctt gttccagacc gcgccgccgt ccctgccgcc gtgtccagga
     5281 tcaaggacca cggtgctgaa gctgagcgcc tgggcggcgc cggcgcaggc gagccagagg
     5341 agaagcagcc acaggcggcg gggaagtgcg gcggtcatgc cggggagaat gcggcatgac
     5401 ggggtgattt gcatcccaaa tcgtcccccg ccaggaacga agcgttcagg atggcgcgcc
     5461 ctgagccggc gcatgggaat gcgccagaat gatttgcagg cattgctcaa tgacgggttc
     5521 gatgccgtcc tggcgcgagt tgtccacctg gacgacgcga acgccgcgcg cctgaagcgc
     5581 ggcgagcacg gcgggatggc agcggatgaa accctgggtg gcaggggcat cagggatgcc
     5641 ggcgctggag cgcaggattt gcccgatttc ctcagagctg agcggcgggt tgcagaattt
     5701 gccgccgaac tgcccgagca tcgcgcgcag ctctgcctcc gggagggtgg acatgagctg
     5761 gcggctgcgc cgggcgttgg cgagcctctg cggcccttcg taaagcttgt gcctgacctt
     5821 gtcccgggtg atgcggcgga cttcgagcac ggcggggtcg gcgacgagct ggatgacgag
     5881 tccgggcagc agatggccgg gcaggaacgg cgcatgcgcc ggggtcatgg acaggcgcag
     5941 aaagccctgg tcaatcaggg ctgtccgaac ctctccgccg agggcgcgcc aggcgatgcc
     6001 atgcgtggaa aaaagatcga gccagacgga gaagcgcgcg atgacgggcc tggcgcggct
     6061 ggcccgccag gcggcgagca catcggccgg gtggctgagg atccagagtc tgcgctgcat
     6121 gtgccgccag cggcgctgac ggcggggcag gtctttttca gggagggcgg ccacgttttc
     6181 catgcccagg atttcgcgca ggcggcgggc caggatgggg gcggcggtgg tttttcccac
     6241 gccgctggcg cctgcgagtt cgacgaggag tttgggagtg gtggacaagt gagcggtgac
     6301 gaaaatgcca cgcatgtgat agccacccgt ttgcgcgggt gcaaccggct tgtcatgcaa
     6361 acaaaaaggc ggcggctccg ggagccgccg ccagagggtg tgagataatc ccgggattta
     6421 ctcgatgaag tagaactggc gggtgttcag cgccacccag agggcgtcgc cgcggccttt
     6481 ctccgcatcg ccctcgatga cgggtgccag cagccctttc tcctcatcgg tggccttgcg
     6541 gctgaagagg gagaggaaga tggtgtcggc ggcggcatcg ggcgtctttg cgcgctccag
     6601 ggcgcgggag atgacggtgt aggggttcag gaggtcgttg aaggtgccgc cgttgagcag
     6661 ggccagggcc tgggtgacgc tggcctcctg gttgccgttg tccacgagtt cgcggtcgct
     6721 ctggccgaag gtgcgcagga agtggccgtt gggggcgggg ctgcgcaggt cggcggcgcg
     6781 gaccatgacg gtgtccctca tgcgctccca ggagagcagc tgggatttgt cctgcttggt
     6841 ggcggcattg aaggtttcag catcgcgctg aagccgggct ttttgctgct cgttgaggcg
     6901 ggctttctgc tggcgggcga cgatattgag ggcgtcgtca atcgtgagtc cctcgtcctg
     6961 ccgggacttg atgagtgcgg tgatctcgcg ggcgaaatcc acatcgtcca cctgctcctt
     7021 gagtttgccc tcgcgcgcca gttcggcaaa ttttttgaag cccatggtat agacgatgtc
     7081 ctgggcggcc tcattgatgc ggctgcgctg ggtgctgacc acgcggcggg cctcgcggat
     7141 ggccttctcg tcgttgtttt cctgggcctt gacgagtttt tcctgggcgg cgcggacttc
     7201 agcggcgagc tgcttttgca gggcggcgat tttggcggca ccggcggcga gttcctggct
     7261 tgagagggcg ttgagggcgc ggtcaagcca ctcgatgcgg cggatggcga cggtgcgctc
     7321 gacgcgggcg tcggcgctgg gcacatccgc gcctggcttg tgcagggtga cgatgctgtc
     7381 ccagatctgc tcggcgctca tgcggcggag aagggggccg ggaaagtggt actgctcgcc
     7441 aagctggaca tcctgcgtgt gcgcggcgcg ctggtaggag gcggagttga agacgacgcg
     7501 caggtaggcc ttcatgtcat agttcacatc cttcatggtc tgctcaagga aggccatgag
     7561 ctgggggttg ctgggaacag tggagtcggt gatctcgtcc acgggttcga tgaggcccat
     7621 gcccatcgcc tttttccaca ggcggttggc gatcaccagg gtgaagcgcg ggttgtcttt
     7681 ggaggccatc cactgcgcgt aggcttcgac gggcttcatg ccttcacggg tgatcttgcc
     7741 gtccttggaa aaagaggcgg ggatcaccgg ctcgatggct tccttcggct tggcgtcgct
     7801 gtattgataa tcatgcggga ggcgcagggt gcgcttgtcc gtctggtcga gcacggtgtt
     7861 gtagcgcagg gggcgcagga tttcggtcat ggccttgccc atgtcctttt tgccggcgcc
     7921 ggccatcatc tccttgttct ttttgccgga gcgcccgtag aaggcgtcgg cgaggagggg
     7981 gttggaaagg ccgttgctgc cggtcatgcc gtaggtgtgg gcggccatct ggtagtaatc
     8041 catctgcgac cacttgtcga aggggtggtt gtggcactgg gcgcagacca tgctggtgcc
     8101 gaggaagatc tgcgtggtca cagccatgtt gtcgagcggc atgttgtagt cgcggatgta
     8161 gaagccgacc gcgccgttct cataagtctt gccgtcggcg ctgacgagct cgcgcaccat
     8221 ctggtcataa ggcttgttgg tcttgaggga ctcgcgcagc cagtcggcgt aggcgtagcc
     8281 cgcgggcaga ctctggccgc cgccgacgga ctggcttttc aagcggagga ggtcggacca
     8341 gaagttgaaa aagtgctgct ggtagccgtc ggaggccagc aggcggtcaa tgagcttctc
     8401 ccgcttggcg gggtcggaat cggcgaggaa atccgtggtc tctttaagcg tggggatgcg
     8461 gcccgcgatg tcgaggtgga tgcggcggac gaagacttcg tcgctggccg gggcgttggg
     8521 ctggaggttt tccttttcca gtttggcgag gatcaggcgg tcaatctcgg cggatttggc
     8581 gcggagatca tcgggagaca tggcttgcgc ggcggctccg aggagaagag cgagaagcgg
     8641 cagcaggagg cggtttttca tgtggcaggg ggggtgtttt tgcggcgcgg ataacggggc
     8701 ttcatggagc ctctttcacg cgcatgaaaa aaagcggcct cccatgcgga ggaagagctg
     8761 acgggtgatt tcatgccagg ctccggcatg cgcgcggcca gcgcatccag ccgcatggca
     8821 tcgttttggt acatcatggc cgttgcttcc gtaaacttgc ctcatgcgaa cctccgcgcg
     8881 ttttttcctc ctcgcccttg tctttgccaa cgtctccgcg caggcctgct cagtgcctgt
     8941 tttccgctat gcgctggagc actggcccgc ggaccctttc caagtgctgg tttttcatca
     9001 tgggccgctc tcaggcggtg atcaggcggc cttgcgtgag gcggagaaaa gggagggggc
     9061 gcgccccaat gtcatcttcc gcacagtgga tctcaatgac gaggcgccgg gggatttgcg
     9121 ccgtttgttt gaatcgcagc agggtgcgcg gacgccgtgg atggtggcgc gttttccatc
     9181 gtcttcgggt attcctgtgc cggtggtctc gcaggccttc actcccgggg cgctcaaagt
     9241 cctgctcgac tcgcccgtcc gcgcggaagt ggtgaagcgg atatccagcg gggagagcgc
     9301 cgtgtggctg ctgctggagt cgggagacaa agctgcggat gacgctgcgg aaaaactgct
     9361 ccaggagcgg ctggattatc tggccagcgt gatgagcctg ccgaagctcg atgattctga
     9421 catcgccaat ggtcttgttt ccgtggccga ggaggacctg cggctggatt tttccctgct
     9481 gcgcatccgc cgggacgacg cggccgagac tgcgctggtg agaatgctgc tgctgagcga
     9541 ggagggtctg gaggaactgg acgagccgat ggtgttcccg gtgttcggcc ggggtcgcgc
     9601 gctgtatgcg ctggccggcg ctggaatccg ccaggagatg atcgagtccg ccgcggcctt
     9661 cctcgtgggc aagtgctcct gccaggtcaa ggagcagaac ccgggggtgg acctgctgat
     9721 gaccgccgac tgggaggctg ccgccaaggc ttcccccgtg cttgaccggg agcttcccac
     9781 gctgcgcgaa ctggtgcccg cccagcctga atcgagcacc atcgaagcag tggcggacgg
     9841 agggtctttt ccggcgtggc gggcatggtc tctgggcatg ggcgtgctgg tgctcgcagg
     9901 aatcgggttc aggctattga ggaaatgaca tccgcgcatg gccgctcgcg cgcaggattt
     9961 gagcgcgccg cggcgtaatt gtcggccatg aatcaagccc gcatttcatc ccgccgtcac
    10021 ttcctcatgc aatccggcct ggtggccgcc gcttccgtgc tgcccgccgc tcgccgggcc
    10081 gcggcctcgc cggacatccc tcatgcgctc gcgggccgtc tttataaaac cctgaagatc
    10141 ggcatggtga aaaccaaggg cacgctcacc gagcgtttca aaaccgccaa agccgccggt
    10201 ttcatgggca tcgagatggg ctttcccggt gacgatgtgc gcgacaccaa ggctgccatc
    10261 gccgcctccg gcctgccggt ggatggcagc gtgtgcgggc agcactggca ggtgcgccac
    10321 accagccccg atcctgaagt ccgcgcgcgc gctttggaaa atctgaaaca ggcgctgcgc
    10381 gacacccgcg aggtgggcgg tcacagcgtg ctgctcgtcg tcggcaaagg cgaggacggc
    10441 cctgaaaatg aaatctggcc gcgctcggtg gagaacatcg ccaaagccat tccgctggcc
    10501 gccgaattgg gagtgcagat cgtcatcgaa aatgtctgga accagttcct ctacaatcat
    10561 gaagggggcg cggaccagac ggcggacaag tttgtcaaat atgtggacga gtttcgctca
    10621 ccctgggtgg ggatgcagtt cgacatcggc aaccattgga aatacggcag catgggagac
    10681 tggattcgcc agctcggcca ccgggtgtac aagctcgatg tgaaggggtt ctcgcgcgcc
    10741 gagggcaagt tcacgccgat tggcgaggga gacattgact atgccgatgt gcgcaaggcc
    10801 ctgcacgaga tcaactttca cggatggctg gccgccgagg tggctggagg cgacctggag
    10861 cacctcaccg gggtggccag gcagatggac gcggccttcg gattgtgatt ccagttgccc
    10921 gccgttcgga aggcgcgcgt caataaactt cttccgtggt gaaagccagg ggctctccgc
    10981 cggggaagat gttgttgtag gcagggtcca tgaaatagac ccggtcgtag cccgcgccgc
    11041 cgtggacatc cacatatccg cggaagtcgt tgccgacggt gttcacggcg gggttgctgc
    11101 ccgccaggaa gatgtcgttg ccagcgccga gataaaccct cacataaccg tggaagacgc
    11161 tgaaaatgga ggagacatcg gtgtcggtgt cgaactcgac aagatcgtct ccattgcccg
    11221 tgtgaatggt cgcattgcgg tcgaagatgc tgtcgcgcac gatcacatcg ctgtcagccc
    11281 cgccgttcat tcgtgtcatc atggcgccgc gcacgtcgct gtcgaggatg cgcacgaggt
    11341 caatgccgcc gaagggagag ttggtggtga tgttgagggc gccgtggatg tcggcgtcgc
    11401 gcacgagcac gtcggcgctg ccggcgccgg tgttcacatt caccgcgccg cgcacggtga
    11461 caagatcgga ggcgccggtg ttttcgccga tgtccaccac gtcgttccca gcgccgccgg
    11521 tgaagcgcac tgagccgacg ctggcgagca cggaattcag tccgaaggcg ttctcccccg
    11581 cgcccgcgtt catggccacc aaaccgccca cgagcgtgtc cgctccgccg atgagcagga
    11641 catccacgcc ggcgccgcca ttgtaggtca gcgcgccgcc gatggtggtg aagcccgtct
    11701 ggtcgagttc gaggcggttg gtccccgcgc cgccgttgaa ggtgagcgtg gtggcgaggt
    11761 caagatcccg tccaagcagg atcagctcat ccgtgcccga tccgccgccg taaaacaagc
    11821 cgcgcgcgaa caggtctccg aggtcgtcgg agcgcaccag gttcactccg ttgccaaggc
    11881 ggatgttgag catcccggca atgtcgagcg tgccgctgag aaagacatga tcctcgccgg
    11941 cgcctgcgct tagattcaaa gccccagtga ctgtgagcag gtcatcgggc aggtcgccga
    12001 tgatgaagtc ggcgggcgcg ttgcctgtgg cgctgagggt gaccgcgccc ttcaccaaac
    12061 cgtcggcggc ggcaagggtg aagctttgga ggtcaaccgg tgtgcccgcg cctttcgctg
    12121 tgatggcggc attggcaagc aggctggtgg cgttcaactc gaaggtgtca gcgcccgcgc
    12181 cgaggttcac attgagtccc cgtccaaaaa acaaatcacc gccggctgtg aatacgtcgt
    12241 cgccgttccc catgcccgca gtgaccgcgc cgttgatgaa ggtggaggtc aggtcaagcg
    12301 tgtcactgcc atcaccggca ttgacggtca gataaccgct catggtcaag ccagacagaa
    12361 gcagcacatc gtcacccgcg ccgagattcg cgcggatgga ggcgggcgcg gcaaaggtca
    12421 cgctggagag caggggacca cgattgaggg agaactcggt ggccgagccg ggcagcgcct
    12481 ccacggtcca catgccgccc gcctcgctga tgccgatctc attgccgaaa gcgtcgcccg
    12541 tgatggtgag caggctgccg ctgaggttca gcgccacgat cccggcggga gcaaggcgtg
    12601 cttcgagagt ttcgagtcgc gcaaatgagg cagggtggtt tttcatgaag gcggggtttt
    12661 gaagattgac gctccgcccc ggcttctatt caacgcaggg cgcgccgttc atggtttccc
    12721 ggcctcgccg cggttgaaca aaggcagggt cagccgcctg gacggcgcgg cctttggcga
    12781 ggattccgtt tcgcgctggg tgcctttgag gatggaagga ctccattggg tgttcttgat
    12841 ggagccctcg ccgcggtatt cgagcagctc gctgaacggg cggaagacaa tgcccggcag
    12901 cccgcgcacg cgcacctggg cggtgaggtc caccttgtcc tcgatgaaat tgatgcttcc
    12961 cgatgaggca agtttgaaga ggttggtcag tgcctcaaag tttgatgtgg tgacaagtcc
    13021 tcccgccact tgaaaagtgc agttggcctc ggtggccaca ttgtagtctt tgatctttcc
    13081 cggcagcacg ctgtcgagca gcggagtgag tgggccgaga atgggaacgg agtaaaggtt
    13141 gccattgaga atgatggccg cgccctctcc tttgagagcc ttccagtcat cctgcctgcc
    13201 ttcgaaacgg aagtggccgg tgacatcgcc ctgggtggcg ctgtctttgg aatacaactg
    13261 cgaaaggcgc tgaaggctca cattttccaa cctcatctct ccagtgtatg gcatcgggtc
    13321 gcgcgactcg tcgagagtgc ctttgatgga gaacgatccc ccaagcagcg cggcacgagt
    13381 gtcgaagcgg atcgttccgc ccttgcccga aacatccgcc gtcactttct caaagggcgc
    13441 gggcttgtcg aagaccctgt aggggaacct ggcggccttt acggcaacgt taaagtcatc
    13501 acgtccttca atgatccaca cgccgccctt ggcgttcatc gccccgccgt ggacacggcc
    13561 gttgatgtcg aagttcagta ctcctccttt gatgtgaagg cggccttcgg gtgagtgaat
    13621 ggtgtgctcc tcgcccagaa cgctgtactt tccagtgccg gttggatgct caaagcgcac
    13681 ctggaagtcg ctgcgcatgc cgtccttgtg aaaaatcacc ccgtccaatt ccacccgcgt
    13741 gtcctcaggc aggcggtact tggcgatgtt cgcggccgtc tttggcgcga aacagctggt
    13801 gaccgccacg gggtcaacgc ccgacttcac acccgtgagg cgcacccact tttcctcgtc
    13861 gtccacctgc acatgagccg cctctgccgc gccctcggtc cggcgcagcc gcacgttgcg
    13921 gtagtgctgg acgggatgtt tgaactcgac atcggcggtc agcagctcca tctccacgcc
    13981 ccggtatttg aagttgcgca gctcgccgcg cccgctgttg aggcattgtt gaagatcggg
    14041 tttcggcccg gcgccttcga gttcaaaaaa gatgttcgag gccggattga actcgaagcg
    14101 gcggatgatc tccctggtct tctccatctg cgcgaaggga atgaaagcgt tcgggtccat
    14161 gcgcagcacg gcgcggtagc ggaagccctg ctcctcatgc gagagcgcct gcgcgctcag
    14221 cgtgccggtc cggtggcgca ggacgatgtc ccggatgtaa acaccctcag gcgccacgcc
    14281 gaacttggcg gagaggccgt caaagacagc tccgcgagtg ctgaagcggc cgcagtcgag
    14341 gctcccgttc aagcgcaccg ggcgcttgtg cgcggacagg tcgccaagca catgccagac
    14401 accatccagc gccaggttcg gactctcata aaacacgacc tcgcgcaaat tgtcgttgtc
    14461 gaggaatgcc agcgccaggg cggggaggtc cgcgctggaa ttgagatgga acctcacctc
    14521 gggcgatccc attcgccagt tggcgttcgc attgaccagg ccggtcgggt ccttgagaaa
    14581 cagccttgtc aaatcaacca cgcccgccct gtattccgcc tcggccacca actcgcggca
    14641 tgtgtaggcg ccgtagctca ggtcgtccgc ctcaaacaca acacgcaagt tcatttcggc
    14701 cagccggtcc atctcaccgc gcaggtccag agacagttgc ggcggactgg caaactggaa
    14761 gcgccgcagc cagtccagcc ctttttgaat tctctgctgg tggttgcgca gcactttgat
    14821 gcgctcctcc acggagaagg aggttttttc ctcgggcttt ttatcctcgt cttttgacgg
    14881 cagcagcatc tctcccgcga tgcggagccg tatgccggag atcgtgcctt ccgcatggcg
    14941 cagttccagg cgcctctccc gcaaaaaggc gcgcgcgctg agatcctcca cctcgatcat
    15001 ggtgtcggcg ccgtcctcgt cctgtccttt cacgggaatg ctcagcctgg ctttgctgag
    15061 ctcgaacccc tgcacctcga agcgccgctc caccagcctg ccgaactcca catccatgcg
    15121 cagcctgtcc accgagacga gcagctcctg gcggtcggag cgtgaataga gccggacatc
    15181 ccgcgccatc agcccgccaa aggggtcgag cacaagacgg cggaagtcca tgtgcaggcc
    15241 gcgctcggcc aactggccgg tcacgaaatc ccgccactgc ctgccgaagg cgtccgtccc
    15301 cgcatagacg atcacggcca gtagagcgct ggccgcggcc agccttatga cgcgccggag
    15361 gaattcgttc atcggcttca gcatagctcc cgcgtcatgg ctggcaacct cagtccccgc
    15421 cgccttgctc ctgtttctgg tgattctccc gagcctgctt gcgcgccgcc gacacgatgt
    15481 ggctgcatgc ttccatgcat agcagcggga tggcccgcgc ctcatccaca ccgggcgcgc
    15541 gtcctgccgc tccgcccagc ttttgcatgg caggccccgg cagtgattgc gagtcatcca
    15601 tgggaaacac gatgcgccgc aggcagcgtg tgcgggtctc acagcagcgc ccgatgattt
    15661 cacccgcctg ctcgtctgtg atgctgttgg cgtagcgata cattcccgtc tgccgcccca
    15721 gcaatacacg cagcggaccg gggcgcagcc ggccctcctg ccatgccagc gccatgcccg
    15781 gagccgcggg gtagaggcag tccagcgcga tccgcaccgc ctccacatcc ggcagcagca
    15841 gacgccagcc gcgccgcagg ttcggcgcgg tcttgatcgg acggaaggct ccctcagcgt
    15901 ccagccgcgc ccattcacgc gccgcttccg gctccgtgca tgtcctcaat tccacagcct
    15961 caccatcctc cacatggcgc agatcatagc cgtccgccgc cgcggtgatc ctcaactgcc
    16021 cgatcacatt ccgcccctcg cccagccagc tctccagcgc cgccgtgatg ccggtgccag
    16081 tgggtggctc tgtgttcacg tcggcaattc cgtgacgcag gtcaaagtcg cgcacctggt
    16141 ccaggatcac atccgtcatc agcggctcgg tgccgatggc gccgctgtag tagagtttgc
    16201 gcccgcgcat ctcgatcgga ttttggcgga agacatccat ctcgctgagc gcctttccgg
    16261 gctcgctctc catgcccagc agcaccggga tgtcctgaaa gctgtggaga ccgtcggcga
    16321 tgaaaaacgg caccaccacc acatgcttcg cgcgcgtcag ctccgcccag tccgacacca
    16381 ggggcggctc ctccatgtaa gcgtccacca cc
//
LOCUS       DAOMYJ010000111        16379 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Prosthecobacter sp. isolate AMR_MDS_5629
            NODE_1225_length_16379_cov_22.754104, whole genome shotgun
            sequence.
ACCESSION   DAOMYJ010000111 DAOMYJ010000000
VERSION     DAOMYJ010000111.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867850
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prosthecobacter sp. (wastewater metagenome)
  ORGANISM  Prosthecobacter sp.
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Verrucomicrobiaceae; Prosthecobacter.
REFERENCE   1  (bases 1 to 16379)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16379)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.30
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:34:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,742
            CDSs (total)                      :: 4,684
            Genes (coding)                    :: 4,676
            CDSs (with protein)               :: 4,676
            Genes (RNA)                       :: 58
            tRNAs                             :: 55
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16379
                     /organism="Prosthecobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1225_length_16379_cov_22.754104"
                     /isolate="AMR_MDS_5629"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1965333"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..368
                     /locus_tag="PLP58_12495"
     CDS             <1..368
                     /locus_tag="PLP58_12495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009964444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="NADH-ubiquinone oxidoreductase-F iron-sulfur
                     binding region domain-containing protein"
                     /protein_id="HRJ09387.1"
                     /translation="GVIIMDDSRSMSWVINNLNAFYAHESCGQCTPCREGSLWMKKIS
                     DRIVAGTASPDDVDTLETVANQIEGKTICAFGEACSWPTQSFIQKFRDELKADCKPEL
                     AGQPVAGEALLAASGLAAR"
     gene            complement(390..1451)
                     /locus_tag="PLP58_12500"
     CDS             complement(390..1451)
                     /locus_tag="PLP58_12500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="terpene cyclase/mutase family protein"
                     /protein_id="HRJ09388.1"
                     /translation="MDRLTFSLAAVFLMLAPGGKARETTPAETPKAAFPQGSPDEPLR
                     GELSLAKAAESLDGTALAWVRKHKCGSCHTGWPYVSSRALLTREAPADALAEVRAFFE
                     GRITNWDADEQVKKHQHREIVGTAAALAMHDAQSTGRLHPVTRQALDRMWALQRKDGA
                     WNWPKCGWPPFEVDDYYGAVLAAVGAGHAPENYARSDAAKAGVDKMRAYLKKTPAPNL
                     HHRAWLLWAAVRLDGLLSDEEKQVIIRDLLALQRVDGGWSMESLGPEWTGREGGKSNP
                     NAPSDGYGTGLVVLVLREAGLPAGHAALQRGVKWLSTNQRESGRWFTRSLNGVPQHFI
                     SDTATVFAVLALKACASGE"
     gene            1623..3128
                     /gene="rseP"
                     /locus_tag="PLP58_12505"
     CDS             1623..3128
                     /gene="rseP"
                     /locus_tag="PLP58_12505"
                     /inference="COORDINATES: protein motif:HMM:TIGR00054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HRJ09389.1"
                     /translation="MQWLTQLGFIGETLYFLILIFEVLMVFNLMILVHEWGHFLAARW
                     RGLKVEAFYIWFGKPLWRKTINGVEYGLGSIPAGGFVKLPQMAPMDAIEGESTSGEPL
                     PPIKPLDKIIVAFAGPLFSFGLACLFALLVSWLGKPQTEPVVTTTIGYVEEDSPAAKA
                     GLLPGDVVRRIDGAEIRRFEGMVDSIRWAVIASEGKEIVFEVERPGEGMKTIPVEADW
                     QEQEPVAWWQGIFKRPPLREVGIMGKGTPMVAEVTANSPADDAGLKPMDELLTVDGQV
                     LYSTTQFMNYLKDKKDRPIKVTIRRKGAEMELELTPRVPDKWESEEKPRVMIGIVFHP
                     TGERKLAYPGVYEQVSDASRAMFSMITKLFTPASDISPGHMSGPIGIGRVYYNLLQDP
                     AALLQVLWFSVVLNINLAILNMLPFPVLDGGHITMAIGEAIRRKPLHSRALEYVQTAC
                     ALLLFSFVIFVTLKDAGDLFLGRRDDGKGTKKEDIGWLPKDQRGKPAAEAK"
     gene            3186..5375
                     /locus_tag="PLP58_12510"
     CDS             3186..5375
                     /locus_tag="PLP58_12510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009962806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1549 and DUF1553 domain-containing protein"
                     /protein_id="HRJ09390.1"
                     /translation="MLRKAFLFICIATAGLAAEPLSFVRDIQPILTKAGCNAGACHAK
                     AITGQRGFRLSVLGFEPEEDYEAIVKQGKGRRVFPPAPEESLLITKGAAIVPHTGGKH
                     LEPGSEFYQTLVRWIAEGMPYTQPNEPVLKEIAVEPSRITLKPKTAQQLKVTARYSDG
                     SSRDVTKLALFEANDTAMATATDSGLVNTLDLPGNVAVMVRFGGKVSVCSVLIPLGAP
                     VESLPPAKNFIDQLIFANLKQIGIPPSPICDDSTFLRRVSLDIGGRLPTMEETKAFLA
                     DKSPDKRDRAIEALINSPDYADYFANKWTALLKNQRTEAADITANFAFHAWMRDNLLA
                     NTPYDQLVRQILASTGTIVSNPPVAWYKRVKEPTTQVEDVAQLFLGVRMQCAQCHHHP
                     FERWTQAEYYHMAAFFSQIGRKPTAIAGEDLIFHKRGTAQTEHRKTRVMLKPAGLGEP
                     PLDIAPDDDPRLALADWMAQKKNPFFAKALVNRYWKHFFKRGLIEPEDDLRDTNPPTN
                     PELLDALAKHFTDNNFDLKSLVRVIAQSHTYQLSAMPNEHNAIDRQAFSHFYPKRLTA
                     EVLLDSIDMVTASKTDFADLPPGTRAISLPDNSYTRASPFLKVFGRPDNTSVCECERV
                     SSASLAQSLHLMNASDVKAKLTASGGRAEQLTKAEMPEPKRIRELYLAAFSREPTADE
                     VQIAETHLLKPRIDATGKPLDSQRAKRNGYEDLLWAILNTKEFLFNH"
     gene            5382..6095
                     /locus_tag="PLP58_12515"
     CDS             5382..6095
                     /locus_tag="PLP58_12515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ09391.1"
                     /translation="MSSRRSNQIGVLLSAWVVLCCAMPGCHRDSAPAQIDLKIIHPDF
                     PKPVFISAPWNCTLPNFEPLRFPPQKEFTAPADAEILSLHKPVTSSRSPSKGQNEWIT
                     DGDAFCGGNGEYAVFFENLPCWVQIDLGSERRIDHVWLWHDQGALFRRATPEVFEDVI
                     VQLSNDVDFGSGVTTLFNSDDDGSCGQGKGTDPTYMETNHGRGVPAKGQTARYVRIWG
                     GKAYSGGPCRFTEVTVWGR"
     gene            6155..6682
                     /locus_tag="PLP58_12520"
     CDS             6155..6682
                     /locus_tag="PLP58_12520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ09392.1"
                     /translation="MRAHTSKDRIDQLMRQMGAAVKSEGSVYFTGGVTAVLMGWRETT
                     IDVDLKAEPEPRGFFECLPRLKDALDINIELAAPDQFVPALPGWQNRSRFIAQHGQLG
                     FYHYDFYGQALSKLERGHPRDHHDVECMRRDGLVKGERLRELFESVEDQLIRYPAINP
                     IQLRAAVMDFCAEVT"
     gene            6691..6996
                     /locus_tag="PLP58_12525"
     CDS             6691..6996
                     /locus_tag="PLP58_12525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ09393.1"
                     /translation="MLDLHDLPAADMIREGLADLAARRESVASYLVQIGSLKMRRCGI
                     EVAVSDEDALDADHRLYALLGREHGNDAHRRYNSLLRELVSFERALVSRHERLQKAR"
     gene            7003..8082
                     /locus_tag="PLP58_12530"
     CDS             7003..8082
                     /locus_tag="PLP58_12530"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HRJ09394.1"
                     /translation="MTRRDTLALTLAALTTPARASNSAMQNAFQLIQTQIDNGTLHHA
                     VLHVQKGSEVTEKAFGKAKPDSMFLLGSITKPLTAAAVMVLVDRGEVKLSDAAVKVLP
                     EFNEGARKDITIEQLLTHTSGLPDQLEDNNALRAHHAPLAEFVKGAVKTPLLFAPGTR
                     YHYQSMGILLAAEIVERVTKTPLPKFLQEHVFTPLGMKRTVLGLGAYSKADMVPMQTE
                     HAAAEAGGGDPNAREWDWNSDYWRNLAAPWGGAHSTAADIANFLRCFMHPDGKALREA
                     TARLMITDHTPKLEAHRGIGFMVGPAGLGKGCSALAFGHSGSTGTLAWADPTTDTSFA
                     LLTSLPKNVSGALVLYPVSDMISPL"
     gene            8138..10309
                     /locus_tag="PLP58_12535"
     CDS             8138..10309
                     /locus_tag="PLP58_12535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009962808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PPC domain-containing protein"
                     /protein_id="HRJ09395.1"
                     /translation="MFPRLICLLSVFAVSALAQQVTLPLPRLLTIMPMGGQAGQSVEV
                     TITGENIEDVTELAFSTTKITAQPVAGAVNKFTVSIAADAPVGVYDARVMSRLGVSSV
                     RAFSVNKLPEVVRTKANNTLETALPLTVGSICNATMTKRAVDFYSFQGVKGQAVAIDC
                     AAVGIDSRLTPVLILADGKGADLKVNRTGGMIDFTPPADGSYIIKVSDLTYQGGERHF
                     YRLALQKSPAEPQPQTQTVSAMSWPPAGLAASAAAKEVEPNNKDAQKISLPCDIGGAF
                     FPAADVDTYEFTAKKGETWWVEVASERLGRNTDPFVLVQQVKDGKFTDVAELYDIAPP
                     MKVTSNGYSYDGPPYDAGSPDVLGKFEVKEDGTYRLQVRDLFGGTRSDPHNVYRLLVR
                     QAAPDFSLAAWAVHMTLRNGDRASLSKPMALRQGDARAFEVVVQRRDGFDGEIEIAME
                     GLPQGVSAAGLKIAKGKTYGHLIVTADEKAPRGFSLAKITGKAVINGKEVTHPVRLAS
                     MEWPVKDAKGEIPAPRLMADIPVSVTDSEQAPLSITLAENKVFEAKAGETLKIPLKLA
                     WRNEFNGTSIKVKAYGEGFSAIKEFDIPLKAATHELALNLAELKIAPGDYTFALQSLG
                     ICKYRYNPAAVPLAEAEQKKAEAALAAAAAEAKKIAATDAEAAKKAAEKQKAAEAAMT
                     AATTRMKSVTTAANPTDTVEILISQPIRVSVKPAAPTTAAK"
     gene            complement(10316..11668)
                     /locus_tag="PLP58_12540"
     CDS             complement(10316..11668)
                     /locus_tag="PLP58_12540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009960877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1501 domain-containing protein"
                     /protein_id="HRJ09396.1"
                     /translation="MPSRRDMLRGSALGFANLAFAGLCAAEAGVQRRSPFDVRAPHFP
                     ARAKRVIFLAMRGAPSQHDLFDYKPRLKELEGQPSEVRGRKYVGLPWEFQQHGQSGQW
                     ISSLLPNLARHADDLCVIKSMKTDDQTHTAGFVQLHTGSARFVRPSLGSWVLYGLGTE
                     NQNLPGFITIRPTVQFGGAQNYGCAFLPASYQGTPIWDPQRENGGVQDIANRRLTRDL
                     QRTQLDLVQEYNAIAAKRDPADTRLDGVIESYELAFRMQVALPEVVDLSKESAATKAA
                     YGIGTTATDGFGRQCLLARRLLESGVRFVEVMDEDWDHHFNLKESLAARCAAIDQPIG
                     ALLDDLKRLGMLDETLVIWGGEFGRTPDNDRADGRNHNSEGFTIWMAGGGVRAGMSYG
                     ETDELGYRAVSNRVHIHDLHATILHLLGFNHTRLTYRHNGREFRLTDVFGDVKHAIIG
                     "
     gene            complement(11689..13482)
                     /locus_tag="PLP58_12545"
     CDS             complement(11689..13482)
                     /locus_tag="PLP58_12545"
                     /inference="COORDINATES: protein motif:HMM:NF019223.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1549 and DUF1553 domain-containing protein"
                     /protein_id="HRJ09397.1"
                     /translation="MTGLPPRWEDVETFEKDASPKAFENLVDRLLRSPRFGERWGRHW
                     LDVARYAETNGRDQNAAYPHAWRYRDYVIDAFNNDVPYDRFIAEQLAGDLLPAKSEPE
                     RDRLMTATGFLAIGGKLMFESNPERFFMEMADEQIQATSLAFMGLSVACARCHDHKFD
                     PITSRDYYALAGIFRSTDVRMGFEYYFPGRPYSPAVITGGAEGRKMAEAHEAHQVIFK
                     QAQREAGQLRNELRQRPAGEREAPEGKALQRRLDEAQARQIAIGKEAPPLPPYVMAVV
                     DRDKPADCHVLEKGEIDSPGELAPRGFPAVLPRGPGIPANASGRLQLAQWLTRPDHPL
                     TARVMVNRIWLHLFGRGLVDTPDNFGTTGSPPSHPELLDELAIRFSEKGWSVKSLIRD
                     IVLSRAYQAGSDADAANQETDAANVYLWRMAPRRLDSEAIRDALLSCAGRLEFTKPES
                     SFNERSSNVQDGSIFRPCRSVYLPVGRENVTSYQRTFDFADPTTCTGLRNETTVPSQA
                     LFFMNSFFVQQQSEFLAKLLITFNEDDDARIQRIFRTVLSRPAAPEELAATHRFLKKL
                     QSQSPAMSRSFTPWTLVCQAVFGSNDFRYLR"
     gene            complement(13439..13711)
                     /locus_tag="PLP58_12550"
     CDS             complement(13439..13711)
                     /locus_tag="PLP58_12550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ09398.1"
                     /translation="MHLHARIPLAMSLLMAADSHAAAETLWSLSAPQRKTAPKTSDAE
                     WSGSATDRWVKAAMEKQGLHPVAHAHPTGFVRSDRSSATMGGRRDF"
     gene            13850..16123
                     /locus_tag="PLP58_12555"
     CDS             13850..16123
                     /locus_tag="PLP58_12555"
                     /inference="COORDINATES: protein motif:HMM:NF019262.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ09399.1"
                     /translation="MKLRSLLLLSCVPAALQADELEFYRDVYPFLKGNCISCHNKTTT
                     KADLNMETPELMIKGGEGGPSLIPGKSAESLIVQASLHANDMEMPPPNNKSGAVNLTP
                     EQIQTLKLWIDQGAKSTKPQERTVVLQAFAASVDPIYCLAISKDGRHAACGRSNRIFV
                     YDLATRQLVAQVGDPAEKNGAAHRALVQSLSFSPDGTRLASGSFREVKLWKLNLAKPL
                     ANSAKSSSAPASPDLIKKIAAAGKVAVLSNAMSADGKQVVTGCNDGSVRVWDAASLKP
                     VIELRGSVATTKKMAELDWTIAAQTLEQTFQKSEITRMEAQDKALDVLLGKANDAITT
                     MKKVLPEKQKAVPPTTEAKVAAQKAVDELTAKFASAKDAAVEKELKTAQDKLITAKMT
                     EVSALAAVSAAESNAKDAEADVTRITASKAANAKAVSAANVAVTAAKTAQDKAAADLA
                     ALKQSLTKTAAKSIAVSFSADALRVASMFDDGTLRVWAAATGTPIEESSATTAATTTI
                     TSSPDGTFVATKAVTQTVGTLPRWTLERKIDQKGVFADRVNAVRFSPDGKTLATGGGE
                     LSRGGDIIFLDVATGKATQTWKEKHSDTVLCLDFSPDGKKLASGAADKIVRVTEIATG
                     KQLNLLEGHTHHVTSVAFRSDGRVLATAGAEGAVVTWDMIIGERKRKIEGWSKEVTSL
                     QFIGATNQIITSAGDNRVRIVNDEGNEIRNIPDLPDYMQAAVSTPNGTTLVAGGEDSL
                     LRIWDSTGKALASFGAK"
BASE COUNT         3191 a         5149 c         5138 g         2901 t
ORIGIN      
        1 gcggcgtcat catcatggac gacagccgca gcatgagctg ggtcatcaac aacctcaacg
       61 ccttctacgc ccacgagagc tgcggccaat gcaccccctg ccgcgagggc agcctgtgga
      121 tgaaaaagat cagcgaccgc atcgtcgccg gcaccgccag cccggacgac gtggacaccc
      181 tggagaccgt ggccaaccag atcgaaggca agaccatctg cgccttcggc gaagcctgct
      241 cctggcccac ccagagcttc atccaaaagt tccgcgacga gctcaaggca gactgcaagc
      301 ccgagctcgc cggccagccc gtcgccgggg aagccctcct cgccgcctcc ggcctggccg
      361 cgcggtgaga ggccgccccg cgccaaggct cattcgccgg aggcgcacgc cttgagcgcc
      421 agcacggcga agacggtggc ggtgtcgctg atgaagtgct gcggcacgcc gttgagcgag
      481 cgggtgaacc agcggccgga ctcgcgctga ttcgtggaga gccatttcac gccgcgctgg
      541 agcgccgcat ggcccgcagg cagtcccgcc tcgcgcagca cgaggaccac caggccggtg
      601 ccgtagccgt cgctgggtgc attcgggttg gatttcccgc cctcgcggcc tgtccattcc
      661 gggccgaggg attccatgct ccagccgcca tccacgcgct ggagggcgag caggtcgcgg
      721 atgatgacct gcttttcctc atcgctgagc agcccgtcga gccgcactgc ggcccagagc
      781 agccaggcgc ggtgatgcag gttgggcgcg ggcgtctttt tcaagtacgc gcgcatcttg
      841 tccacaccgg ccttcgcggc atcgctccgc gcgtagtttt cgggcgcatg ccccgcgccg
      901 acggcggcca gcacggctcc gtaataatca tccacctcga acggcggcca gccgcacttc
      961 ggccagttcc acgcgccgtc tttgcgctgc agcgcccaca tgcggtcgag cgcctggcgc
     1021 gtcacgggat gcagcctgcc ggtggactgc gcatcgtgca tcgccagcgc cgccgcggtg
     1081 ccgacgatct cgcggtgctg gtgctttttc acctgctcat cggcgtccca gttcgtgatg
     1141 cggccctcga aaaaggcgcg cacctccgcc agcgcgtccg caggcgcttc ccgcgtcagc
     1201 aacgcgcgcg aactcacata cggccagccc gtgtggcagg agccgcactt gtgcttccgc
     1261 acccaggcca gcgccgtgcc atccagcgac tccgcggcct tcgcgagcga gagctccccc
     1321 cgcagcggct cgtctggcga tccctgggga aaggctgcct ttggcgtctc tgcgggcgtc
     1381 gtttcccgcg ccttgccacc cggcgcgagc atcaggaaaa cggcggcaag gctgaatgtg
     1441 aggcggtcca ttgggcaatg catggcgtcc atgctgaacg gcaggcggag cgccaacttg
     1501 cgccgcgtga tcgcggcccc gggcggacgc gcctgaaaac aatcgcactc ggcgtttgcc
     1561 ctcggggcgg cgggtgttag ctttgcggcc cgcgcgcatc cggcgcggct ttttttaccc
     1621 ctatgcaatg gctcacccaa ctcggtttca tcggcgagac gctctatttc ctgatcctca
     1681 tcttcgaggt gctgatggtc ttcaacctga tgatcctggt gcatgagtgg ggccatttcc
     1741 tggcggcgcg ctggcgcggg ctgaaggtgg aggcgtttta catctggttc ggcaagccgc
     1801 tgtggcggaa gacgatcaac ggcgtggaat acggcctggg cagcatcccg gcgggcggtt
     1861 ttgtgaagct gccgcagatg gcgccgatgg acgccatcga gggcgagtcc accagcggcg
     1921 agccgctgcc gccgatcaag ccgctggaca agatcatcgt ggccttcgcg gggccgctgt
     1981 tcagcttcgg gctggcctgc cttttcgccc tgctggtgtc ctggctgggc aagccgcaga
     2041 cggagccggt ggtgacgacg acgatcggct atgtggagga ggacagcccg gcggcgaagg
     2101 cggggctgct gcctggggat gtggtgcgcc ggattgacgg cgcggaaatc cgccgcttcg
     2161 agggcatggt ggacagcatc cgctgggccg tcatcgccag cgagggcaag gagatcgtgt
     2221 tcgaggtgga gcgccccggc gagggcatga agacgatccc ggtggaggcg gactggcagg
     2281 agcaggagcc ggtggcgtgg tggcagggca ttttcaaacg cccgccgctg cgggaggtgg
     2341 gcatcatggg caaggggacg ccgatggtgg cggaagtgac ggcgaacagc cctgcggatg
     2401 acgcgggact gaagccgatg gacgagctgc tgacggtgga cggccaggtg ctctacagca
     2461 cgacccaatt catgaattac ttgaaggaca aaaaggaccg gcccatcaag gtgacgatcc
     2521 gccgcaaggg cgcggagatg gaactggagc tgacgccgcg tgtgccggac aagtgggaat
     2581 ccgaggaaaa gccgcgcgtc atgattggca tcgtttttca tccgacgggg gagcggaagc
     2641 tggcctatcc cggcgtctat gagcaggtgt cggatgcctc gcgggcaatg ttcagcatga
     2701 tcacgaagct gttcacgccc gcctcggaca tcagccccgg ccacatgagc gggcccatcg
     2761 gcatcgggcg cgtgtattac aatctgctgc aggaccccgc cgctcttctc caggtgctgt
     2821 ggttcagcgt ggtgctgaac atcaacctgg ccattctgaa catgctcccc ttccccgtgc
     2881 tggacggcgg ccacatcacc atggccatcg gcgaggccat ccggcgcaag ccgctgcact
     2941 cgcgcgcgct ggagtatgtg cagacggcgt gcgcgctgct gcttttcagc ttcgtcattt
     3001 tcgtcacgct caaggacgcg ggcgaccttt tcctcggccg gcgggatgac ggcaagggca
     3061 cgaagaagga ggacatcggc tggctgccga aggaccagcg cggcaagccc gcagcggagg
     3121 cgaagtgagg cgtggcggct ttgtccggca tgaaaaaccg ccgcttcatc gcgtaggttt
     3181 ccatcatgct tcggaaagcc ttcctcttca tttgcatcgc cacggccggt ctcgccgcgg
     3241 agcctttaag cttcgtgcgg gacatccagc ctattttgac gaaggccggc tgcaacgcgg
     3301 gcgcttgtca tgcaaaggcg atcaccggtc agcgcggctt tcggctcagc gtgctcggtt
     3361 ttgagccgga ggaggactac gaggccatcg tgaagcaggg caaaggccgc cgcgtgttcc
     3421 cgccggcgcc ggaggaaagc ctgctcatca ccaaaggcgc tgccatcgtg ccgcacacgg
     3481 gcggcaagca cctcgaaccc ggctccgagt tttatcagac gctcgtgcgc tggatcgccg
     3541 agggcatgcc ttacacgcag ccgaacgagc cggtgctcaa agaaatcgcc gtcgagccct
     3601 cacgcatcac gttgaagccg aaaaccgcgc agcagctcaa agtcaccgct cgctactccg
     3661 acggcagcag ccgcgatgtg acgaagctcg cgctgtttga ggccaacgac accgccatgg
     3721 ccaccgcgac cgacagcggc ctcgtgaaca cgctcgatct gccgggcaat gtcgccgtca
     3781 tggtgcgctt cggcggcaaa gtgagcgtgt gcagtgtgtt gatcccgctc ggtgcaccgg
     3841 tcgagtcgct gcctccggcg aagaacttca tcgaccagct catcttcgcc aatttgaagc
     3901 agatcggcat cccgccctcg cccatctgcg atgacagcac cttcctccgc cgcgtctcgc
     3961 tcgacatcgg tggccgtttg ccgaccatgg aagaaacgaa ggccttcctc gccgacaaat
     4021 cgcccgacaa acgcgaccgg gccatcgaag cgctaatcaa ctcgcccgac tacgccgact
     4081 acttcgcgaa caaatggacc gcgctgctca aaaaccagcg caccgaggcc gccgacatca
     4141 ccgcgaactt cgccttccac gcctggatgc gcgacaacct gctcgcgaac acgccttacg
     4201 atcaactcgt ccgccagatc ctcgccagca ccggcaccat tgtttcgaat ccacccgtcg
     4261 cctggtataa acgcgtcaaa gaacccacca cgcaggtcga ggacgtcgcg cagcttttcc
     4321 tcggcgtgcg catgcagtgt gcgcagtgcc atcatcatcc cttcgagcgc tggacgcagg
     4381 ccgagtacta tcacatggcc gccttcttca gccagatcgg ccgcaaaccg accgccatcg
     4441 ctggtgagga cctcatcttc cacaagcgcg gcaccgcgca gaccgagcat cgcaaaaccc
     4501 gcgtcatgct caagcccgcc ggcctcggcg aaccaccgct cgacatcgcg cccgatgacg
     4561 atccgcgtct cgcgctcgcc gattggatgg cgcagaagaa aaaccctttc ttcgccaaag
     4621 cgctcgtgaa ccgctactgg aagcacttct tcaaacgcgg cctcatcgag cccgaggacg
     4681 acctccgcga caccaatccg ccgacgaacc ccgaactgct cgacgctttg gcaaaacact
     4741 tcaccgataa caacttcgac ctcaaatccc tcgtccgcgt catcgcgcag agccacacct
     4801 accagctcag cgccatgccg aacgagcaca acgccatcga ccgccaggcc ttctcccact
     4861 tctacccgaa acgcctcacc gccgaggtcc tgctcgacag catcgacatg gtcaccgcgt
     4921 ccaaaaccga cttcgccgac ctcccacccg gcacccgcgc catttcactg cccgacaaca
     4981 gctacacccg cgcctcgccc ttcctcaaag tcttcggtcg tcccgacaac accagcgtct
     5041 gcgaatgcga acgcgtctcc tccgccagtc tcgcgcaaag cctgcacctc atgaacgcga
     5101 gcgacgtcaa agccaagctc accgccagcg gcggccgcgc cgagcaactc accaaagccg
     5161 agatgcccga gcccaaacgc atccgcgaac tctacctcgc cgccttctcc cgcgaaccca
     5221 ccgccgacga agtccaaatc gccgaaaccc acctcctcaa gccccgcatc gacgccaccg
     5281 gcaagcccct cgattcccaa cgcgccaaac gcaacggcta cgaagacctc ctgtgggcca
     5341 tcctgaacac gaaggagttc ttgtttaatc attgaggtgc catgagcagc cggagatcga
     5401 atcagattgg ggtgctctta tccgcatggg tggtgctttg ttgcgccatg cctggatgcc
     5461 accgggacag cgcacccgca cagattgacc tgaaaatcat tcatcctgat ttccccaagc
     5521 cggttttcat ttcagcgccc tggaactgca ctctgccaaa ctttgagccg ctgcggtttc
     5581 ccccgcaaaa ggagttcaca gctcccgcgg atgcggaaat tctttctctc cacaaacctg
     5641 tcacctcgtc cagatcacct tcaaaaggac aaaatgaatg gatcactgac ggtgatgctt
     5701 tttgcggagg aaatggcgag tatgccgttt tcttcgagaa tctgccgtgc tgggttcaga
     5761 ttgatctcgg ctcagaacgc cgaatagatc acgtctggct ctggcatgat caaggagcgt
     5821 tgttccgcag ggcgaccccc gaagtcttcg aagatgtcat cgtgcagctc tcgaacgacg
     5881 tggattttgg atctggcgta accacgctgt tcaattccga cgacgacggc tcctgcggcc
     5941 agggcaaagg cactgatccg acctacatgg agaccaatca tggccgtgga gtgcccgcaa
     6001 agggccaaac agctcgatat gtccgtatct ggggcggcaa agcctattcc ggcggcccct
     6061 gtcgcttcac cgaggtgacg gtgtggggcc ggtgatgcgg gcccatgccg aagaaaaggt
     6121 tttggctgcc acttgtgaca ctataacttt cgtcatgcgt gcccacacca gcaaagaccg
     6181 gatcgaccag ctcatgcgcc agatgggcgc ggcggtgaag tcggaaggta gtgtgtattt
     6241 caccggtgga gtcacagctg tgctgatggg ctggcgtgaa acgaccattg atgtcgattt
     6301 gaaggccgag ccggagccgc gaggcttctt tgagtgtctg ccacggctca aagacgcact
     6361 cgacatcaac atcgagctcg ccgcacccga tcagttcgtg cctgcgttgc ccggttggca
     6421 gaacagaagc cgtttcatcg ctcaacacgg ccaacttgga ttctaccact acgatttcta
     6481 cgggcaggca ctctccaagt tggagcgcgg tcatccgcgt gatcatcacg atgtcgaatg
     6541 catgaggcgg gatggtctgg tgaagggtga gcggctccgg gaactgtttg agagcgtcga
     6601 agatcagttg atccgctacc ctgccatcaa cccaattcag cttcgggccg ccgtgatgga
     6661 cttttgcgcc gaagtgacct aacctttcac atgcttgacc tccacgatct ccccgctgcg
     6721 gacatgatcc gcgaaggcct cgccgatctg gcggcgcgcc gtgagagtgt ggcgtcgtat
     6781 ctcgtgcaga tcggcagcct caaaatgcgg cgatgcggca tcgaagtggc cgtctcggac
     6841 gaagatgcgc tcgacgccga tcatcgactc tatgctctgc taggccgcga acacggcaac
     6901 gatgcccacc ggcgctacaa ttcgctgctg cgcgaactcg tcagtttcga gcgtgcgctc
     6961 gtctcacggc atgaacgcct gcaaaaagcg cgttaagcct gcatgacccg ccgcgacacc
     7021 ctcgctctca ctttggctgc gctcacgaca ccggcgcgtg ccagcaactc cgccatgcaa
     7081 aacgcctttc agctcatcca gacgcaaatc gacaacggca cgctgcatca cgcggtgctg
     7141 catgtgcaaa agggctcgga agtgacagag aaggcctttg gcaaggcgaa gccggattcg
     7201 atgttcctgc tcggttcgat tacaaaaccg ctcacagccg cagccgtaat ggttttggtg
     7261 gatcggggtg aggtgaagct ttccgacgcc gcggtgaagg ttctgccgga gttcaacgaa
     7321 ggcgctcgaa aggacatcac catcgagcaa ctgctcacgc acacctccgg cctgccggac
     7381 cagcttgagg acaacaacgc actgcgggca caccacgcgc cgctggcgga gtttgtgaag
     7441 ggcgcggtca aaacgccgct gctcttcgcg ccgggcacgc ggtatcacta tcaaagcatg
     7501 ggcatcctcc tcgcggcgga aatcgtcgag cgggtgacga agacgcctct gccgaagttc
     7561 ttgcaggagc atgttttcac gccgctgggc atgaaacgca cggtgttggg cctcggagcc
     7621 tattccaagg ccgacatggt gccgatgcaa accgagcacg ccgctgccga ggcaggcggc
     7681 ggtgatccga acgcccgcga gtgggactgg aacagcgatt actggcgaaa cctcgccgcg
     7741 ccgtggggtg gcgctcacag caccgcagcg gacatcgcca actttctgcg ctgcttcatg
     7801 catcccgatg gcaaagcgct gcgcgaggcc accgcacggc tcatgatcac cgatcacaca
     7861 cccaaactcg aagcgcatcg cggaatcggc ttcatggtcg gccctgccgg tctcggcaaa
     7921 ggttgctccg ccctcgcctt cggccacagt ggctccacgg gcacgctggc ctgggctgat
     7981 ccaacgacag acacgagctt tgccttgctg acgtcactcc caaagaatgt ctcgggcgcg
     8041 ctggtgctgt atccagtctc tgacatgatt tcgccgcttt gagcaagact gcgggacgcg
     8101 ccaagaacag ccggtttcgt cgcgtagttt tgcggtcatg tttccgcgac tcatctgcct
     8161 cctctccgtc tttgccgtct ccgcgctcgc gcagcaggtc acgctgccgt tgccgcgact
     8221 gctcacgatc atgcccatgg gcggtcaggc cgggcagagt gtggaggtga cgatcacggg
     8281 tgaaaacatc gaggacgtga cggagctggc cttctccacc acgaaaatca cggcgcagcc
     8341 ggtggcgggt gcggtgaaca agttcacggt gagcatcgcg gcggatgcgc cggtgggtgt
     8401 ttacgatgcg cgggtgatgt cgcggctcgg ggtgtcgtcg gtgcgggcgt tttcggtgaa
     8461 caaactgccc gaagtggtgc gcacgaaggc gaacaacacg ctcgaaacgg cgctgccgct
     8521 caccgtcggt tcgatctgca acgcaacgat gacgaagcgt gcggtggatt tttattcgtt
     8581 ccagggcgtg aaggggcagg cggtggcgat tgattgcgcg gcggtcggga tcgactcgcg
     8641 gctcacgccg gtgctcatcc tcgcggatgg caagggcgcg gacctcaagg tgaatcgcac
     8701 aggcggcatg atcgacttca caccgcccgc cgatggcagc tacatcatca aggtgagcga
     8761 tctgacctat cagggcggcg aacggcattt ttatcggctc gctttgcaaa agagccccgc
     8821 ggagcctcaa ccgcagacac aaaccgtcag cgcgatgtcc tggccgcctg ccgggctcgc
     8881 ggcgagcgcg gcggcgaagg aagtggagcc gaataacaag gacgcgcaga agatttcact
     8941 gccctgcgac atcggtggcg cgtttttccc ggcggcggat gtcgatacat atgagttcac
     9001 cgcgaagaag ggcgagacgt ggtgggtgga ggtcgcgagc gagcgtcttg gccgcaacac
     9061 cgatcccttc gtgctcgtgc agcaggtgaa ggacggcaaa ttcaccgatg tggccgagtt
     9121 gtatgacatc gcgccgccga tgaaggtcac gagcaatggc tacagctacg acgggccgcc
     9181 ttacgatgcc ggttcgccgg atgtgctcgg caaattcgag gtcaaagagg acggcaccta
     9241 tcgtctgcaa gtgcgcgatc tgtttggcgg cacgcgcagc gatccgcaca acgtgtatcg
     9301 actgctcgtg cggcaggccg cgccggattt ctcgctcgcc gcgtgggcgg tgcacatgac
     9361 gctgcgcaat ggcgaccgcg cctcgctttc gaagccgatg gcgctgcgtc agggcgatgc
     9421 gcgggcgttt gaggtcgtgg tgcagcgtcg cgatggcttt gatggcgaga tcgagatcgc
     9481 gatggaaggc ttgccgcagg gtgtcagtgc cgccggtttg aaaatcgcga agggcaagac
     9541 ctatggtcat ctcattgtca cggcggatga aaaagcgccg cgtggcttct cgctggccaa
     9601 aatcaccggc aaggccgtta tcaatggcaa ggaagtcacg catcccgtgc gtcttgcgag
     9661 catggaatgg cccgtgaagg acgcgaaggg cgaaatcccc gcgccgcggc tcatggcgga
     9721 cattccggtg tccgtgaccg attccgagca ggcgccgctg tcgatcacgc tggctgaaaa
     9781 caaggtcttc gaggccaaag ccggcgagac gctgaaaatc ccgctcaagc tcgcctggcg
     9841 gaacgagttc aacggcacct cgatcaaggt gaaggcctac ggcgaaggct tcagcgccat
     9901 caaggagttc gatatcccgc tgaaggccgc cacgcatgag ctggcgctga atctggccga
     9961 attgaagatc gcgccgggcg attacacctt cgcgctgcaa agcctcggca tctgcaaata
    10021 ccgctacaac cccgctgcgg tgcctttggc cgaggcggag caaaagaaag ccgaggccgc
    10081 cctcgccgcc gcagctgccg aagccaaaaa gatcgccgcc acggatgccg aggccgcgaa
    10141 gaaagccgcc gagaagcaaa aggctgctga agcggcgatg acggcggcga cgacccggat
    10201 gaaatcggtc accaccgctg cgaatcccac ggacacggtc gagattctca tctcgcagcc
    10261 gattcgcgtg agcgtgaagc ccgccgcgcc gaccacggcg gcgaagtgaa gcgcgtcatc
    10321 cgatgatggc gtgtttcaca tcgccgaaca cgtcggtgag gcggaattcg cggccgttgt
    10381 ggcggtaggt gagcctggtg tggttgaatc cgaggaggtg caggatggtg gcgtgcagat
    10441 cgtggatgtg gacgcggttg ctcacggcgc ggtaaccgag ttcgtcggtc tcgccgtagg
    10501 acatgccagc acgcacgccg cctccggcca tccagatcgt gaagccttcg gaattgtgat
    10561 tgcggccgtc ggcgcggtcg ttgtcgggcg tgcggccgaa ttcaccgccc cagatgacga
    10621 gcgtttcatc gagcatgccg agacgtttga gatcgtcgag cagcgcgccg atgggctgat
    10681 cgatggcggc acagcgagcg gcgagacttt ccttcaaatt gaagtggtgg tcccagtcct
    10741 cgtccatgac ctcgacgaaa cgcacgcccg actccagcag gcggcgtgcg aggaggcact
    10801 ggcggccaaa gccgtccgtc gcggtcgttc cgatgccgta agcggccttg gtggcggcgc
    10861 tttccttcga caaatccacg acctccggca gcgcgacctg catgcggaag gcgagttcat
    10921 aggattcgat gacgccgtcg agccgcgtgt cggcgggatc gcgtttcgcg gcgatggcgt
    10981 tgtattcctg cacaagatcg agttgcgtgc gctgcaagtc gcgggtgagg cgacggttgg
    11041 cgatgtcctg cacgccgccg ttctcgcgct gcggatccca gatgggcgtg ccctgatacg
    11101 aagcgggaag aaaggcgcag ccatagttct gcgcgccgcc gaactgcacg gtgggccgga
    11161 tggtgatgaa gccgggcagg ttttgattct ctgtgcccag gccgtaaagc acccacgaac
    11221 ccagcgaagg gcgcacaaaa cgcgcggagc ctgtgtggag ctgcacaaag ccggcggtgt
    11281 gcgtctggtc gtcggtcttc atgctcttga tgacgcacag gtcatcggca tgacgcgcaa
    11341 gattgggcag cagcgaggag atccactggc cgctctggcc gtgctgttgg aattcccacg
    11401 gcaggccgac gtatttgcgc ccgcgcacct cgctcggctg gccttccagt tccttgagcc
    11461 gcggtttgta gtcaaaaaga tcgtgctgag agggcgcgcc acgcatggcg aggaagatca
    11521 cgcgtttggc acgcgccgga aaatgcggcg ctcgcacatc aaacggcgag cgacgctgca
    11581 cgcccgcttc cgcggcgcac aggccggcga acgcgagatt tgcaaaccca agcgcgctgc
    11641 cgcgcagcat gtcgcggcgg ctgggcatga agagtggagt tcgcggattc atcgaagata
    11701 acggaaatca ttgctgccaa acaccgcctg gcacaccagc gtccagggcg tgaaggatcg
    11761 cgacatcgcc ggcgactggc tttggagttt tttcaggaag cgatgcgtgg cggcgagctc
    11821 ctccggcgct gccggacggc tcagcaccgt gcggaagatg cgctggatgc gggcgtcgtc
    11881 gtcttcgttg aaagtgatga ggagtttggc cagaaactcg gactgctgtt gcacgaagaa
    11941 gctgttcatg aagaacagcg cttgcgacgg cacggtggtt tcattgcgca gcccggtgca
    12001 ggtggtggga tcggcaaagt cgaaggtgcg ctggtagctg gtgacgtttt cgcggcccac
    12061 cggcagataa acggagcggc agggacgaaa gatggaaccg tcctgcacat tggaggagcg
    12121 ttcattgaac gaagactccg gcttggtgaa ctcaagacgg ccggcgcagg agagcaacgc
    12181 gtcgcggatc gcctccgagt cgaggcggcg cggcgccatg cgccacagat atacattggc
    12241 agcatcggtt tcctgattgg ccgcatccgc gtcgctgcca gcctgatagg cgcggctcag
    12301 gacgatgtcg cggatgagcg atttcacgga ccagcctttt tcactgaagc ggatggcgag
    12361 ttcgtcgagc aactcagggt ggctcggtgg cgatcccgtc gtgccgaagt tgtccggcgt
    12421 atcgaccagt ccgcggccga acagatgcag ccagatgcga ttcaccatca cgcgggcggt
    12481 gagcggatga tcaggtcgtg tgagccattg cgcgagttgc aagcggccgc tcgcattcgc
    12541 cggaatgccg gggccgcgcg gcagcacggc gggaaaacca cgcggcgcga gctcgcccgg
    12601 cgagtcgatc tcgcctttct ccagcacatg gcagtccgcc ggtttgtcac gatccaccac
    12661 ggccatcacg taaggcggca gcggcggcgc ttctttgcca atggcgatct gtcgtgcctg
    12721 cgcctcgtcg aggcggcgtt gcagtgcttt gccctcgggc gcttcgcgtt caccggcggg
    12781 tcgttgccgc aattcattgc gaagttggcc ggcttcgcgt tgtgcctgct tgaagatgac
    12841 ttgatgcgcc tcatgggcct ccgccatctt cctgccttcc gctccgccgg tgatgaccgc
    12901 gggcgaatag ggcctgccag gaaagtagta ttcaaatccc atgcgcacgt ccgtgctgcg
    12961 gaagatgccc gccagcgcgt agtagtcgcg gctggtgatg ggatcgaact tgtgatcatg
    13021 gcagcgtgcg caggccacgg acaggcccat gaacgcgagg ctcgtcgcct ggatttgctc
    13081 gtcggccatc tccatgaaaa agcgctcggg attgctctcg aacatcagct tgccgccgat
    13141 ggcgaggaag ccggtggccg tcatcaggcg gtcgcgttcc ggttccgatt ttgcaggcag
    13201 cagatcaccc gcaagctgct cggcgatgaa gcggtcatac ggcacgtcat tgttgaacgc
    13261 gtcgatgacg tagtcacgat aacgccacgc atgcggataa gcggcgtttt gatcgcgacc
    13321 gttcgtctcc gcatagcgcg ccacatccag ccagtgccgg ccccagcgtt cgccaaagcg
    13381 cggcgaccgc agcaagcggt ccaccagatt ttcaaaagcc ttgggcgagg catccttttc
    13441 aaaagtctcg acgtcctccc atcgtggcgg aagaccggtc agatcgaacg aaacccgtcg
    13501 gatgagcgtg cgccacggga tgaaggccct gcttctccat cgccgccttc acccaacggt
    13561 cggtcgccga gcccgaccac tcggcatcgc tggtcttcgg agcggtcttt cgctgcggag
    13621 ccgacaacga ccacagcgtc tccgccgcgg catgggaatc tgccgccatc agcaggctca
    13681 tagccagggg gattcgtgcg tgcaggtgca tgggacgtgg ttggaacgtg cgacgggagc
    13741 ccgtgtttag aacactccat gtgtacgcca ttctttccgt gatacccacg tttcctgctg
    13801 aatgctccgg cctttttgag ccgtaggttt gccagtctca tccacattcg tgaagcttcg
    13861 ttctctactt ctcctgtctt gcgttccggc ggccctccag gccgacgagc ttgagttcta
    13921 ccgcgatgtt tatccgtttc tgaaagggaa ctgcatctcg tgccacaaca agacgacgac
    13981 gaaggccgat ctaaacatgg agacgccgga gttgatgatc aaaggcggcg agggtgggcc
    14041 gtcactcatt ccggggaaaa gcgcggagag cctcatcgtg caggcttcgc tgcacgcgaa
    14101 tgacatggag atgccaccgc cgaacaacaa gtctggcgcg gtgaatctca cgcccgaaca
    14161 gatccaaaca ctgaagctgt ggatcgatca gggcgcgaaa agcacaaagc ctcaggagcg
    14221 cacggttgtg cttcaggcct tcgccgcgag cgtggatccc atctactgct tggcgatttc
    14281 caaagatggc cgccatgccg cctgtgggcg atcgaaccgc atcttcgtgt atgatctcgc
    14341 cacgcgtcag cttgtggcgc aggtgggcga tcctgcggag aaaaacggtg cggcgcatcg
    14401 cgcactggtg cagtcgctgt ccttcagccc cgatggcact cgcctggcga gcggcagttt
    14461 tcgcgaggtg aagctctgga agctgaattt ggcaaagccg ttggcgaact ctgcaaagtc
    14521 atcgtccgct cccgccagtc ccgatttgat caagaaaatc gccgccgcag gcaaagtggc
    14581 cgttttgagc aatgcgatgt cggcggatgg caagcaggtc gtcaccggct gcaatgatgg
    14641 atccgtgcgt gtgtgggatg cggcgtcgct gaagccggtt atcgagctgc gaggcagtgt
    14701 ggcgaccaca aagaagatgg cggagttgga ctggaccatc gccgcgcaga cgctcgagca
    14761 gacgtttcaa aagagcgaaa tcacccgcat ggaagcgcag gacaaggcgc tcgacgtgct
    14821 gctcgggaag gcgaacgacg cgatcaccac gatgaagaaa gtgctgcctg agaaacagaa
    14881 ggcggtgccg ccgaccaccg aggccaaggt cgccgcgcag aaggctgtgg atgagctcac
    14941 ggcaaaattc gcctccgcga aagatgcagc cgtcgagaaa gaactcaaaa ccgcccagga
    15001 caagctcatc accgcaaaga tgaccgaggt ttccgctctc gctgctgttt ccgccgctga
    15061 gagcaatgcg aaggacgccg aggccgatgt gacgcgcatc accgcatcga aggcggcgaa
    15121 tgccaaagcc gtctcggctg ccaacgtggc cgtcaccgct gcgaaaaccg cgcaggacaa
    15181 ggccgccgcc gatctcgcgg cgctgaagca gtcgctgacc aaaactgccg ccaaatccat
    15241 cgccgtctca ttttccgccg acgcccttcg tgtggcttcg atgtttgacg atggtacgtt
    15301 gcgcgtgtgg gccgccgcca ccggcacgcc gattgaagaa agcagcgcga ccaccgctgc
    15361 gaccaccacg atcacatcat cgcccgacgg cacctttgtc gccacgaagg ccgtcacgca
    15421 gaccgtgggc acgctgccgc gttggacgtt ggagcgcaag atcgaccaga aaggtgtgtt
    15481 tgccgatcgt gtgaatgccg tgcgcttcag tccggatggc aaaacgctcg ccaccggtgg
    15541 tggcgagttg tcgcgcggcg gcgacatcat tttcctcgat gtcgccaccg gcaaagccac
    15601 gcagacttgg aaggaaaaac acagcgacac ggtgctctgc ctcgatttct cgcccgatgg
    15661 caagaagctg gcgtcgggcg ctgcggacaa gatcgtgcgc gttactgaga tcgccacggg
    15721 caaacaattg aacctgctgg aaggtcacac gcatcacgtc accagcgttg ctttccgcag
    15781 cgatggccgc gtgctcgcca ccgcgggcgc ggaaggcgct gtggtgacgt gggatatgat
    15841 catcggcgag cgaaagagaa agatcgaagg ctggagcaag gaggtcacct cgctgcagtt
    15901 cattggcgcg accaaccaga tcatcacctc cgccggcgac aaccgcgtcc gcatcgtgaa
    15961 cgacgaaggc aacgagatcc gcaatatccc cgacctcccc gactacatgc aggccgccgt
    16021 cagcacgccg aacggcacta ccctcgtcgc cggcggcgag gacagcctgc tgcggatctg
    16081 ggacagcacc ggcaaggcgc tggcttcgtt tggtgcgaag taacagggtc tttccagtct
    16141 gtgttcagac ggactggcca aggaatcagc ccaagcctcc gggttgactc tggaaatctc
    16201 ataccaaacc gattcactca tcgcagcatg atggcgggca ggctgtaacg ggagcatcat
    16261 ggcacgagga gccatccaac tggggcggct ggcagaagac gcgctgcatc tcaaagaact
    16321 cgcagcgagc gatgctgagg cagtgcgtgt cgtcatccgc gacaacgccg ggttccatc
//
LOCUS       DAOMYJ010000112        16315 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Prosthecobacter sp. isolate AMR_MDS_5629
            NODE_1232_length_16315_cov_19.084317, whole genome shotgun
            sequence.
ACCESSION   DAOMYJ010000112 DAOMYJ010000000
VERSION     DAOMYJ010000112.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867850
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prosthecobacter sp. (wastewater metagenome)
  ORGANISM  Prosthecobacter sp.
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Verrucomicrobiaceae; Prosthecobacter.
REFERENCE   1  (bases 1 to 16315)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16315)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.30
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:34:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,742
            CDSs (total)                      :: 4,684
            Genes (coding)                    :: 4,676
            CDSs (with protein)               :: 4,676
            Genes (RNA)                       :: 58
            tRNAs                             :: 55
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16315
                     /organism="Prosthecobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1232_length_16315_cov_19.084317"
                     /isolate="AMR_MDS_5629"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1965333"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(139..1455)
                     /locus_tag="PLP58_12560"
     CDS             complement(139..1455)
                     /locus_tag="PLP58_12560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009958974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1552 domain-containing protein"
                     /protein_id="HRJ09400.1"
                     /translation="MKHLHRRQFLRDLGVSAAALPFLTGLPGVTGAPAPQKRQRLVIM
                     FSPNGTLPGEFWPDQEGAGFSFKSILQPLESFKDRMLILHGIANKVRGDGDSHMRGMS
                     CLLTCDELLKGNIMGGGGNPAGWAGSISIDQEIKNFLQSRPDTRTRFGSLEFGVAVPD
                     RADPWTRMCYAGANQPVAPMDDPHQVLKKVYGKMKDKESLVSILDDVRADLKRVSARL
                     SARDKALLDQHMELVRGLEKDLEAADKMGALSHPVPDVDPSIELVNDNTPQISRIQID
                     LLVNSLTNNMARVATLQYMRSVGMAQMRWLGVEEGHHSLSHDPDDNKDSYEKLKKINT
                     WFAGEFAHLAKRLAETPEPAGGGSMLDNTLLVWTNELGKGNSHTLDNIPFVMVGGGFG
                     FKMGRSLKFDKAAHNRLWLAIARAMGHDLQTFGKKELCEGGPLVLG"
     gene            complement(1533..4703)
                     /locus_tag="PLP58_12565"
     CDS             complement(1533..4703)
                     /locus_tag="PLP58_12565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009962160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1553 domain-containing protein"
                     /protein_id="HRJ09401.1"
                     /translation="MKILLSALVLTSPALFGAAGKAEFNRDIRPILADACFHCHGPDP
                     GTRKAGLRLDTEAGFFTAKEGETPTVIKGKPEDSPLYQRLITKDEDDIMPPPESHKEL
                     TPEQIAKVKAWIEEGAPWQAHWSLIAPQRPAVPEVRSSKFEIRNPVDAFVLAKLQEAG
                     LEPAPEADAHTLVRRLSLDLTGLPPTPDLIQRYCQPGQSGQSRLTDTQYSALIDELLK
                     SPAYGEHRARYWLDAARYADTHGLHFDKYREIWPYRDWVVSAFNRNQPFDQFTIEQIA
                     GDLLPQPNEQQLIATGFQRCNITTNEGGTIDEENLANYAADRVQTLGWVYMGLTTNCS
                     QCHDHKFDPISQRDYYSLAAFFRNTTQKPKDGNVKDGLGPVLVLPDAKDRPRWDALPA
                     EIATAKTKLDENRKLARPEFDTWLTSAKPEDLDKDIPAQGMVAHVPLNEGAGSEVTGV
                     CGSAKTFKATGEVSWKPDGKIGPAPVMKPNGTFDIGDAGDFEKDKPFSYGAWVRAGKL
                     GISGGILARMDEKGAYRGWDLWQNDRNYAVHIIHRWPDDALKVSTAKPTVRPGVWQHV
                     FVTYDGSGKAEGVKIYIDGEAQPLKTEVNALKNTIRTATPTRIGQRSHVQVFHEGSVQ
                     DVRIYERLLRPAEIQTLAKVGPLREMLASAKRGPKQVDALFAHYLATRHQGWIAADKT
                     HKALEAEQAAIKNRSPMTHVQEEKMDSQPMANILMRGQYDQVGEQVPAEVPAALGKLP
                     QGAPRNRLGLAQWLVSAENPLTARVTVNRLWQEIFGQGLVKTSEDFGIMGMPPTHPEL
                     LDWLAVEFRESGWDVKRLVKLIVSSSTYRQAANTTPEKLAKDRDNSLLSRGPRFRMDA
                     EMIRDYALAASGTLSPRMGGPGTYPYQPENVWEVVGMGTEKYAQDKGENLYRRTLYNF
                     WKRMAPPASLDVFNAPSRETSCVRRDRTNTPLQALVVMNDVQFVEASRNLAQRVLASA
                     EKDDARLDELALRLLCRPLKPAERQVLSASLADLQTHYAAHPEDAKALITVGETPPDA
                     KLPAPALAAWTMVCNQVMNLDEVLNQ"
     gene            complement(4786..5115)
                     /locus_tag="PLP58_12570"
     CDS             complement(4786..5115)
                     /locus_tag="PLP58_12570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ09402.1"
                     /translation="MPRPRPMELHRHPSGLYYSRQFADYLRSKQAEEEARHPGEILSL
                     EYVRCREGEQAGASWLRLAWVSLFSKMAEQCLDIEAIRIALHRQTQRGLKNRLLHYAD
                     GQVLVKR"
     gene            complement(5235..6818)
                     /locus_tag="PLP58_12575"
     CDS             complement(5235..6818)
                     /locus_tag="PLP58_12575"
                     /inference="COORDINATES: protein motif:HMM:NF013188.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HRJ09403.1"
                     /translation="MTELALLLLLAAIGHGLSRWTGLPVIPLLIGLGMAFSFSGLAAH
                     PGAEGGVEDDPLFFVLELGLTVLVFASGVELNPQRFRRQRRAVLWVGLLQFTVMGVVG
                     WAGALALGFAGMPAVYLAFAVSASSTIVVLRQLQVSQQSFEPHGRMVVGVLLLQDALT
                     IVTLVALTHLGAGVAPLALGLLYAALMAAAAWLLQWRGARWLIERCRSDEEAMLLVVL
                     SVLFVFLGASGLAGLPLVVGAFLAGYGLSSFPVSGLVGGLLQSLADFFRAIFFVALGA
                     LVVVPQPGAVGVAAVLALLVLVITPPLVAAVAEWMGLTSRSAIESGLLLAQTSEYSLV
                     LGLVGLRFGHIDAEVFAIVAMMAVATMTCTPLLARESVARRLMRLHPLRRRRRLPALE
                     CREHVLVLGLGSAGMWVLKPLQSAGVPILVVDDDPIVIAELDKKGVPCLRGDGADEHT
                     LERIGARHARLIIASMRRVSDAQKVLAHAKGVPVLARVFEDHEAEAIRRLGGIPVSNS
                     EAAVEQFMKWFQAGQKTAA"
     gene            complement(6815..8494)
                     /locus_tag="PLP58_12580"
     CDS             complement(6815..8494)
                     /locus_tag="PLP58_12580"
                     /inference="COORDINATES: protein motif:HMM:NF013188.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HRJ09404.1"
                     /translation="MHEETFIDELGWIVVGATLFAFLARRIGMPSIVAYLLAGLGLGP
                     ATGLVEMSAELSLISEIGIALLLFLVGLELSFDKIRDVGKVAVVAGIGQVVFTAAGGL
                     ILCWLLGFPLMEAVFLSVGLTFSSTVVVVKLLDEKGELDSLYGRIAVGIFLVQDLVAI
                     LILTFLAGLGGGAGGGVALDAGAVLKGLGGAFGGMILLLAAALLAARYVLPKPFAWSA
                     RRPEVLLIWSLSWCFALVMGAHALHLSLEIGAFLAGISLAQLPYNEDLHRRVHPLMNF
                     CIAVFFVSLGVKMEIGEAVAEWPTVTVLSLFVLIGNPAIFLWIIARMGYGLRTTFYTS
                     VTVAQISEFSFIVVALGVSSGLVGEGVMSLTALVGLVTITFSAYMIRFSRPLYEWVVR
                     LGLHKPFVPRRLWQVDEQYAAEEAFAPHGLEGHIIVVGMNSLGRRLARELTAAGHTVV
                     AVDTDPHKLAGLPCRTELGNVGYRAVLDHLCLRRARLLVSALRIDATNDLLAYRCREA
                     GVPCAMHVHDVREMDQLLEIGTDYLMISKVDGVKRLNTQLKQMGCLPGKTP"
     gene            8599..9471
                     /locus_tag="PLP58_12585"
     CDS             8599..9471
                     /locus_tag="PLP58_12585"
                     /inference="COORDINATES: protein motif:HMM:NF012993.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrilase-related carbon-nitrogen hydrolase"
                     /protein_id="HRJ09405.1"
                     /translation="MVLDIQTYDPGFVCDSAREYARHVTKRVLDSWDEGADLVLLPEF
                     LWMGLEPHTPDEPEGSALERVARLFREELLPWLRAALARPGKAVVLGTCPCAVEGGIR
                     NRAVIFHDGKETHQDKLHLTPWEKEFTPGGSVRLWEFGGLLVAVIICLDIEIPELAAR
                     LRGLGVDLILCPSATETILGVERVDRCASARAVELGCFVAVAHLTGRARSDLIDENVG
                     RAAFYQPSQHVFRHTPRWIEGPVHDSGCHALRVTVDKEALKKMRRMRAETNPSLLGKD
                     LAGLSRGIPVECAR"
     gene            9489..9860
                     /gene="mscL"
                     /locus_tag="PLP58_12590"
     CDS             9489..9860
                     /gene="mscL"
                     /locus_tag="PLP58_12590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009962060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="large conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HRJ09406.1"
                     /translation="MLNEFKKFILKGNVVDLSTGVIIGAAFGNIVTAFTKGIVEPLIK
                     LASGGTDPNVTLKLWIFDVGVVINALIGFLITATVIFFVIVKPVNKLLSMMKKEEAAA
                     PPPPTPADIALLTEIRDLLKK"
     gene            complement(9935..10915)
                     /locus_tag="PLP58_12595"
     CDS             complement(9935..10915)
                     /locus_tag="PLP58_12595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ09407.1"
                     /translation="MKTYPLTTLLAAAGFGVLIAPGQTPAQQPYQPAPQYQRPPGYSA
                     PPAYPQQQQPQYSQPVPRTEYVSPMEFLPTFGRKFGDMFRRLFYGDKPPSGYQQGGYP
                     QYGGRSLDQAPGYYQSQPRQYQQAQPQYYQPQYAPPQYQQPQYAQPQTPRYETPPAQQ
                     QRPPAQGTPAQTRPAQPQSPPVTSKSKSPASSTKTGAATPAKKYTPPTITRPSESKSA
                     GTPPSTAGAAKTDTPPKTDTASAARTEYFPLPGSQPKDTPKDATASAGKPKSDSPPAA
                     NTGAFLKGKRTAKEGRVTSPYPPYQELDVTGLGSGSLALDPTTNKVFEVP"
     gene            complement(11057..11914)
                     /locus_tag="PLP58_12600"
     CDS             complement(11057..11914)
                     /locus_tag="PLP58_12600"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012705.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004659 - prenyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phytoene/squalene synthase family protein"
                     /protein_id="HRJ09408.1"
                     /translation="MSDTALTPAQIARKAKSNLALALACLPAQRRHDMIAFYAFCRVV
                     DDIADSPHLSEAEKEGRLHEWKRCVSEGRAPGHPVLDEVVVLPRKYGFPRAWLAEIID
                     GVASDIGRVRYETYEELLAYCYKVASVVGLVSAEIFGCQNPASRDYAIKLGYALQLTN
                     IMRDVGQDARETGRIYLPLEDLRKFSVTESEILDGRHNQRFVRLMDFEHERAAKLFKD
                     AEKMLPADDRRHLLPAAMMARIYSEILEKLRRERYQVFTRRCRLHPLRKGWILCSYLA
                     RAWMGRRGR"
     gene            12330..13817
                     /gene="dnaA"
                     /locus_tag="PLP58_12605"
     CDS             12330..13817
                     /gene="dnaA"
                     /locus_tag="PLP58_12605"
                     /inference="COORDINATES: protein motif:HMM:TIGR00362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA];
                     GO_process: GO:0006275 - regulation of DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HRJ09409.1"
                     /translation="METNPDECPPAPNHHSHNGAASTQELFDVVPGGWDRICEILHRK
                     LGEDNFSRCFAGTSARVTGGGRLVISVPNPIHQLWIESNFQGVLNDAAAEVLGTAARI
                     EFSVALESAQPFSKDAAPQDVKAARVVGTRQGEPRNQAPSGIPEPVSERALIEAGLNP
                     KYNFDSFVVGANCSYSAAVARAVAEKPGRIYNPLFLHGATGLGKTHLMQAIGQEVLMR
                     KKRAVVRYVTSEQFTNEFVEAIKKQNFTLFRQKYRKVDLLLIDDVHFFGGKDSTQEEF
                     FHTFNELFNNARQIVLASDRPPAEMKNLEARLVSRFDWGLTTQIQTPDLETRTAILRH
                     KVAGFNVTLAPWIIEFIAQRVRTNVRKLEGALMRVASRVSLEGDIPNEVALAAFLDDV
                     IEDEPAKSVTVDRVQRAVATQYDLRVSDLTGPRRPKNIAEARQVAMYLTRQMVKLPLI
                     QIGEEFGGRDHGTVIHACKVVSKRMHQKDDFRIMVDRLSAKLLEA"
     gene            13954..14172
                     /locus_tag="PLP58_12610"
     CDS             13954..14172
                     /locus_tag="PLP58_12610"
                     /inference="COORDINATES: protein motif:HMM:NF026183.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-binding protein"
                     /protein_id="HRJ09410.1"
                     /translation="MHLHGDRMVRRAFVPGGRSTLQLKDVRLICELAESLGFCSPTLE
                     NCRAQWETFVHEKGGGDLDHSGFFRMSE"
     gene            14831..15220
                     /locus_tag="PLP58_12615"
     CDS             14831..15220
                     /locus_tag="PLP58_12615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ09411.1"
                     /translation="MIEALIEIFFEFGLQIVIELFAEWGTRSTVRRREGGPPPAWLAG
                     VGYFVLGGLLGGASLLVFPAHWVTDPGLRLVNLLVTPVIAGLLMSALGAWRRSRGQEL
                     LRLDKFTHGWLFALALALVRHFGARAA"
     gene            15274..>16315
                     /locus_tag="PLP58_12620"
     CDS             15274..>16315
                     /locus_tag="PLP58_12620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ09412.1"
                     /translation="MPRKFIFLLALATSMTASAQEMRVFTNSQGVAIKAALVSVAGGQ
                     ATIRREDGAQFTVAVTAFSAADQEYIRQWKPAAAPSAPSARPSAPGPNDALDAAALNA
                     LAGHELFGDSPLWDAPAEDAAKRLGLDRESQTSTQSSYRSYPPDDYRLFGARPHSVAL
                     YAEDGKTASLSLVYANKGDLFSARGGSEDHFDKDTPPAEAAKIVKKAMDADLKTIAET
                     LSAKLGEPKKERFGEGKAGRMNMQRWDWRGHSILLAEAEGEYVGVQVVPAAFADAGGK
                     SVRVSDAAIRERAKANVQTRPNGDVVIQDIPMVDQGPKGYCVPATAERAMRYLGVAAD
                     MYVLANAGETGYG"
BASE COUNT         2753 a         4884 c         5769 g         2909 t
ORIGIN      
        1 gggagggtac gcgcggcggg cgcggcttcg cgcaacgcct gcggcggccg cgcccgcaag
       61 ggcgtggtgg tgcgggtgtg aggggcgctg cttttgccaa tccccaccgc cttgcggcga
      121 cggctacgcc gcggagcctc agccgaggac gagggggccg ccttcgcaga gctccttttt
      181 gccgaaggtc tggaggtcat gccccatggc gcgggcgatg gcgagccaga ggcggttgtg
      241 ggcggctttg tcgaacttga ggctgcggcc cattttgaag ccgaagccgc cgccgaccat
      301 gacgaagggg atgttgtcga gggtgtggct gttgcctttg ccgagctcgt tggtccagac
      361 gaggagggtg ttgtcgagca tggatccccc gccggcgggc tcgggggtct cggcgaggcg
      421 tttggcgagg tgggcgaact cgccggcgaa ccaggtgttg atctttttga gcttttcgta
      481 gctgtccttg ttgtcgtcgg ggtcgtggga gaggctgtgg tggccttcct cgacgccgag
      541 ccagcgcatc tgggccatgc cgacgctgcg catgtattgc agggtggcga cgcgggccat
      601 gttgttggtg agggagttga cgaggaggtc tatctggatg cggctgatct ggggggtgtt
      661 gtcgttgacg agttcgatgc tggggtccac gtcgggcacg gggtgggaaa gcgcgcccat
      721 tttgtcggcg gcttcgaggt ctttctccaa accgcggacg agctccatgt gctggtccag
      781 gagggctttg tcccgcgcgc tgagcctggc ggagacgcgc ttgaggtcgg cgcgcacgtc
      841 atcgaggatg ctgacgaggc tttccttgtc cttcattttg ccatagacct ttttgagcac
      901 ctggtggggg tcgtccatgg gggcgacggg ctggttggcg ccggcgtagc acatgcgggt
      961 ccaggggtcg gcgcggtcgg gcacggcgac gccgaactcg agggagccga agcgggtgcg
     1021 ggtgtcgggg cggctttgga ggaagttttt gatctcctgg tcaatggaga tgctgccggc
     1081 ccatccggcg gggttgccgc cgccgcccat gatgttgccc ttgagcagct cgtcgcaggt
     1141 gagcaggcag ctcatgccgc gcatgtggct gtcgccgtcg ccgcggactt tgttggcgat
     1201 gccgtggagg atgagcatgc ggtccttgaa ggactcgagc ggctggagga tggacttgaa
     1261 gctgaacccg gcgccctcct ggtcgggcca gaactcgccg ggcagggtgc cattgggcga
     1321 gaacatgatg acaaggcgct ggcgcttctg aggcgcgggc gcgccggtga cgccgggcag
     1381 cccggtgagg aagggcaggg cggcggcgga gacgcccagg tcgcggagga attgacggcg
     1441 gtggaggtgc ttcatgaggg gtgaggaagc tacggcagcc ggagggctgt cttgcctgtg
     1501 aaatgacgcc tccgggtgcg ggagggatgc tgctattgat tgagcacctc gtcgaggttc
     1561 atgacctggt tgcagaccat ggtccaggcg gcgagggcgg gggcgggaag tttcgcgtcg
     1621 ggcggggtct cgccgacggt gatgagggct ttggcgtcct cggggtgcgc ggcgtagtgc
     1681 gtttgcaggt cggcgaggct ggcgctgagg acctggcgct cggcgggctt gagcgggcgg
     1741 cagagcaggc gcagggcgag ttcatcaaga cgggcgtcgt ctttttcggc ggaggcgagg
     1801 acgcgctggg cgaggttgcg ggaggcttcg acgaattgca cgtcgttcat gacgacgagg
     1861 gcctggagcg gggtgttcgt gcggtcgcgg cgcacgcagc tcgtctcgcg gctgggtgcg
     1921 ttgaagacat cgaggctggc gggcggggcc atgcgcttcc agaagttgta aagggtgcgg
     1981 cggtagaggt tctcgccttt gtcctgggca tacttttcgg tgcccatgcc gacgacttcc
     2041 cagacgtttt caggctggta ggggtaggtg ccggggccgc ccatgcgggg ggagagggtg
     2101 ccgctggcgg cgagggcgta gtcgcggatc atctcggcat ccatgcggaa gcgcgggccg
     2161 cggctgagca gggagttgtc ccggtctttg gcgagttttt ccggcgtggt gttggcggcc
     2221 tggcggtagg tggagctgct gacgatgagc ttgacgagcc gcttcacatc ccagccgctc
     2281 tcgcggaact cgacggcgag ccagtcgagc agctcggggt gcgtgggcgg catgcccatg
     2341 atgccgaagt cctcgctggt cttgacgagg ccctggccga agatctcctg ccagaggcgg
     2401 ttcacggtga cacgggcggt gagcggattt tccgcgctga cgagccactg ggcgaggccg
     2461 aggcggttgc gcggcgcgcc ttgcgggagc ttgccgaggg cggcggggac ttcggcgggc
     2521 acctgctcgc cgacctggtc atactggccg cgcatcagga tgttcgccat cggctggctg
     2581 tccattttct cctcctggac gtgggtcatg gggctgcggt ttttgatggc ggcctgctcg
     2641 gcttcgaggg ctttgtgcgt cttgtccgcc gcgatccagc cctgatgacg cgtggcgagg
     2701 tagtgggcaa agagggcgtc cacctgcttc ggcccgcgct tggcgctggc gagcatctcg
     2761 cgcagcgggc cgactttggc gagggtctgg atttccgccg ggcgcagcag gcgctcgtag
     2821 atgcgcacgt cctgcacgct gccctcgtgg aagacctgca catggctgcg ctggccgatg
     2881 cgtgtggggg tggcggtgcg gatggtgttc ttgagcgcgt tgacctcggt cttcaaaggc
     2941 tgcgcctcgc cgtctatgta aatctttacg ccttccgctt tgccgctgcc gtcataggtg
     3001 acgaagacgt gctgccagac gccggggcgc acggtgggct tggcggtgct gactttgagc
     3061 gcgtcgtccg gccagcggtg gatgatgtgg acggcgtagt tgcggtcgtt ctgccagagg
     3121 tcccagccgc ggtaggcgcc cttttcatcc atgcgggcga gaatgccgcc gctgatgccg
     3181 agcttgcccg cgcgcaccca ggcgccgtag ctgaagggct tgtctttctc aaagtcgccc
     3241 gcgtcgccga tgtcgaaggt gccgttcggc ttcatgacag gcgcggggcc gatcttgccg
     3301 tcgggtttcc agctcacctc gccggtggct ttgaaggtct tcgcggagcc gcagacgccg
     3361 gtgacttcgc tgccggcgcc ttcattgagc ggcacatgcg ccaccatgcc ctgggcgggg
     3421 atgtctttgt cgagatcctc gggcttggcg gaggtgagcc aggtgtcgaa ctcgggccgg
     3481 gccagtttgc ggttctcgtc gagcttcgtt ttggcggtgg cgatctcggc gggcagggcg
     3541 tcccagcgcg ggcggtcctt ggcatcgggc agcacgagca cgggtccgag gccgtccttc
     3601 acgttgccat ccttgggctt ctgcgtggtg ttgcggaaaa aagcggcgag cgagtagtag
     3661 tcgcgctggg agatggggtc gaacttgtgg tcgtggcact gggagcaatt ggtggtcagg
     3721 cccatgtaca cccagccgag ggtctgcacg cggtcggcgg cgtagttggc gaggttttcc
     3781 tcatcaatgg tgccgccctc gttggtggtg atgttgcagc gctggaagcc ggtggcgatg
     3841 agctgctgct cgttgggctg cggcagcagg tcgccggcga tctgctcgat ggtgaactgg
     3901 tcaaagggct gattgcggtt gaaggcggag accacccagt cgcggtaagg ccagatctcg
     3961 cggtatttgt caaagtgcag tccgtgcgtg tcggcataac gcgcggcatc cagccagtag
     4021 cgggcgcggt gctcgccgta ggcggggctt ttcagcagtt cgtcaatgag ggcgctgtat
     4081 tgggtgtcgg tgagacggga ctggccggat tgacctggct gacaatagcg ctggatcaga
     4141 tcgggcgtgg gtggcagtcc ggtgaggtct aacgataggc ggcggacaag agtgtgcgcg
     4201 tcggcttcag gcgcgggttc gaggccggcc tcctggagtt ttgccaggac gaaggcatcc
     4261 acagggtttc gaatttcgaa tttcgagctt cgaacttccg gcactgccgg cctttgcggg
     4321 gcgatgagcg accaatgcgc ctgccaggga gccccttcct cgatccaggc tttgaccttg
     4381 gcgatctgct ccggcgtcag ctccttgtgc gactcgggcg gcggcatgat gtcatcctca
     4441 tcctttgtga taaggcgctg gtagagcggg ctgtcctcgg gcttgccttt gatgacggtc
     4501 ggcgtctcgc cctccttggc ggtgaaaaag cccgcttccg tgtcgaggcg cagcccggct
     4561 ttgcgcgtgc ccgggtccgg cccgtggcag tggaagcagg cgtccgccag gatggggcgg
     4621 atgtcgcggt tgaactcggc cttgcccgcc gcgccgaaga gggcggggga ggtgagcacg
     4681 agggcggaga ggaggatttt catatcaatc acggcggcaa aaatacgagt tgcgcgtcct
     4741 tgtgtttgca gcggcggcgg gattatccat cggcggggcc gggagtcagc gtttcacgag
     4801 cacctggccg tcggcatagt gcaggaggcg gtttttcaag ccgcgctgag tctggcggtg
     4861 gagggcgatg cgaatggctt cgatgtcgag gcattgctcg gccattttac tgaacaggct
     4921 cacccaggcc aggcgcagcc aggaggcgcc ggcctgctcg ccctcgcggc agcgcacata
     4981 ctccagggag aggatttcgc cgggatgccg cgcctcctcc tccgcctgct tcgagcgcag
     5041 gtagtcggca aactggcgcg agtaatacaa cccgctggga tgacggtgca gttccatcgg
     5101 acggggacgc ggcattctgg cgcgccaaca gcggctgcca agaaaaaatg gctgtcaaag
     5161 atgaagttcg gatatgcgcg ccaggggggc atttcacctc tggcggggct ttgcatggaa
     5221 acccggacgc cgggtcaggc ggcggttttc tgcccggcct ggaaccactt catgaactgc
     5281 tcgaccgcag cctcggaatt ggagacgggg atgccgccga ggcggcggat ggcctcggcc
     5341 tcgtggtcct cgaagacgcg cgccaggact ggcacgcctt tggcatgggc caggactttt
     5401 tgggcgtcgg acacccggcg catggaggcg atgatgaggc gggcatgacg cgcgccgatg
     5461 cgctccaggg tgtgctcgtc ggcgccgtcg ccgcgcaggc agggcacgcc ttttttgtcc
     5521 agttcggcga tgacgatggg gtcgtcatca acgacgagga tgggcacgcc tgcggattgc
     5581 aggggtttga gcacccacat gccggcggag ccgagtccaa gcacgagcac atgttcccgg
     5641 cactcgagcg cgggcaggcg tctgcggcgg cgcaggggat gcaggcgcat gaggcggcgc
     5701 gccacggact cgcgcgccag cagcggcgtg caggtcatgg tggccacggc catcatcgcg
     5761 acgatggcga agacctccgc gtcaatgtgc ccgaagcgca gccccacgag gccgaggacg
     5821 agggaatatt cgctggtctg cgccagcagc aggccgctct cgatggcgga gcggctggtc
     5881 agccccatcc actcggccac ggcggccacc agcggcgggg tgatgacgag cacgagcagc
     5941 gccagcacag cggccacgcc gaccgcgccc ggctgcggca cgaccacgag cgcgccgaga
     6001 gccacgaaga agatggcgcg gaaaaagtcg gccagggatt gcagcaggcc gccgaccagg
     6061 ccgctgacgg ggaagctgga aagaccgtag cccgcgagaa aggcgcccac cacgagcggc
     6121 aggccggcca ggccggacgc gccgagaaag acgaacagga cggagagcac caccagcagc
     6181 atggcctcct cgtcactgcg gcagcgttca atgagccagc gcgcgccgcg ccattgcagc
     6241 agccaggcgg cggcggccat gagggcggcg taaaggaggc cgagcgcgag cggcgccaca
     6301 cccgcgccga gatgggtgag ggcgacgagg gtgacgatgg tcagcgcgtc ctgcagcagc
     6361 agcacgccga cgaccatgcg cccgtgcggc tcgaaggact gctggctcac ctggagctgc
     6421 cgcagcacca cgatggtgga gctggcggac acggcgaagg ccagataaac ggccggcatt
     6481 cccgcgaagc cgagcgccag cgcgccagcc cagcccacca cgcccatgac ggtgaactgg
     6541 agcagaccga cccagagcac cgcgcggcgc tggcggcgga agcgctgggg gttgagctcc
     6601 acgccggagg cgaagacgag cacggtgagg ccgagttcga ggacgaaaaa aagcgggtca
     6661 tcctccaccc cgccctccgc gcctggatgc gcggccagcc cgctgaagga gaaggccatg
     6721 cccaggccga tgagcagggg gatgacgggc agccctgtcc agcgggagag gccgtggccg
     6781 atggcggcga gcagcaggag cagggcgagt tccgtcatgg ggttttgccg ggcaggcagc
     6841 ccatttgttt gagctgggtg ttgaggcgct tcacgccgtc cactttggag atcatcagat
     6901 agtcggtgcc gatctccagg agctggtcca tctcgcgcac atcatgcaca tgcatggcgc
     6961 agggcacgcc ggcctcgcgg cagcggtagg ccaggaggtc gttggtggcg tctatgcgca
     7021 gggcggagac cagcaggcgg gcgcggcgca ggcagaggtg gtcgagcacg gcgcggtagc
     7081 cgacatttcc cagctcggtg cggcagggca ggccggcgag cttgtgcggg tccgtgtcca
     7141 cggcgacgac ggtgtggccg gcggcggtga gctcgcgcgc gaggcggcgg ccgaggctgt
     7201 tcatgccgac gacgatgata tgcccctcca gcccgtgggg cgcgaacgct tcctcggcgg
     7261 cgtattgctc gtccacctgc cacagccgcc gggggacgaa gggcttgtgc aggccgaggc
     7321 ggaccaccca ttcatacagg ggcctgctga agcggatcat gtaggcgctg aaggtgatgg
     7381 tgaccaggcc gacgagcgcg gtcagggaca tgacgccctc ccccaccagc ccggaactca
     7441 cgccgagtgc cacgacgatg aatgagaact cgctgatctg cgccaccgtg acgctggtgt
     7501 agaaagtggt gcgcagtccg tagcccatgc gggcgatgat ccacagaaag atggccggat
     7561 tgccaatgag cacaaagagg gaaagcacgg tgaccgtggg ccactccgca accgcctcgc
     7621 cgatctccat cttcacgccc agggagacga agaagacggc gatgcagaaa ttcatcagcg
     7681 gatgcacgcg gcggtgcagg tcctcattgt aggggagctg ggccaggctg atgccggcaa
     7741 gaaaggcgcc gatctccagg gagaggtgca gcgcgtgcgc gcccatgacc agggcaaagc
     7801 accacgagag gctccagatg agcagcacct ccggccgccg cgccgaccag gcgaagggct
     7861 tcggcagcac ataacgcgcc gccagcagcg ccgccgccag cagcaaaatc atgccgccaa
     7921 aggcgccgcc gagtcccttg agcacggctc ccgcatccag ggccacgccg ccgcccgcgc
     7981 cgccgccgag gccggcgagg aaagtgagga tgaggatggc cacgaggtcc tgcaccagga
     8041 agatgcccac ggcgatgcgc ccgtagaggc tgtccagctc gcctttttca tcaagcagct
     8101 tcaccaccac caccgtgctg ctgaaggtga ggccgacgga gaggaaaacg gcctccatga
     8161 gcgggaagcc gagcagccag cacaggatga ggccgcctgc ggcggtgaag accacctggc
     8221 cgatgccggc gacgacggcg acttttccca catcccggat tttgtcgaag ctcagctcca
     8281 gaccgacgag gaaaagcagc agggcgatgc cgatttccga gatgagcgag agttcggcgg
     8341 acatctccac cagccccgtc gccggcccga gccccagtcc cgcgagcagg taggcgacga
     8401 tggagggcat gccgatgcgc cgcgccagga acgcgaacaa cgtcgctccc accacgatcc
     8461 agcccagttc gtcaatgaag gtttcttcgt gcatttccct cttcgtggta ggcgcgagga
     8521 gggatgttct caacccgcaa agccgtgaca ggtggatttt ttgagtgcgc gttcatcatc
     8581 cagggctttg ctgcgcgcat ggttctcgac atccagacct atgatcccgg cttcgtttgc
     8641 gattccgcga gggaatacgc ccggcatgta acgaagcggg tcttggattc atgggatgaa
     8701 ggcgcggacc tggtgctgct gccggagttt ctgtggatgg gcctggagcc gcacacgccg
     8761 gacgagcctg aaggatccgc tcttgaacgg gtggcgcggc tcttccggga ggaactgctt
     8821 ccctggctgc gcgctgcttt ggcacgccct ggcaaggcgg tggtgctggg cacatgcccg
     8881 tgtgctgtgg agggcggcat tcgcaacagg gcggtgattt ttcacgatgg gaaggagacg
     8941 catcaggaca aactgcacct gacgccgtgg gagaaggaat tcacgcccgg cggcagtgtg
     9001 cggttgtggg agttcggcgg gctgctcgtg gcggtgatca tctgcctgga catcgagatt
     9061 cctgagctgg cggcgcgtct gcgcggattg ggggtggacc tcattctgtg cccgagcgcc
     9121 acggagacga tccttggcgt ggagcgtgtg gaccgctgcg cctcggcgcg ggcggtggag
     9181 ctgggatgct tcgtggccgt ggcgcatctg acagggcgcg cgcgctcgga tctcatcgac
     9241 gagaatgtgg ggcgcgcggc attttaccag ccctcgcagc atgtcttccg ccacacaccg
     9301 cgctggattg aggggccggt tcatgacagc ggctgccatg cgctgagggt gactgtggac
     9361 aaggaggcgc tgaaaaagat gcggcgcatg agagccgaaa cgaatccctc cctgcttggc
     9421 aaagatttgg ctggtctttc ccgcggcatt ccggtggaat gcgcccgata aatcaaacca
     9481 tcctccccat gctcaacgaa ttcaaaaagt tcatcctcaa aggcaacgtc gtggacctct
     9541 ccaccggtgt catcatcgga gccgccttcg gcaacatcgt caccgccttc accaagggca
     9601 ttgtggagcc gctgatcaag ctggccagcg gcggcacaga tccgaatgtc accctgaagc
     9661 tgtggatttt cgacgtcggt gtcgtgatca acgcgctcat cggctttctc atcacggcca
     9721 cggtgatctt tttcgtcatc gtcaagccgg tgaacaagct gctgtccatg atgaagaagg
     9781 aggaggctgc cgcgccgccg ccgcccactc cggcggacat tgcattgctc acggaaatcc
     9841 gcgacctgct gaagaagtaa ggacgcgaaa agcccgggga gggtttccct gctccgggct
     9901 tttttgcgac tggcgagact ggcgaaagaa gggatcaggg cacctcgaag actttgttgg
     9961 tcgtcgggtc aagcgcgagc gagccgctgc cgaggccggt gacatccaac tcctggtagg
    10021 gagggtaggg gctggtgacg cggccttcct tggcggtgcg tttgcctttc aaaaaggcgc
    10081 cggtgttggc ggcgggtggt gagtcgcttt tcggtttgcc ggcggatgcg gtcgcgtcct
    10141 ttggcgtgtc cttgggctgg gagccgggca gcgggaagta ttccgtgcgc gcggcgctgg
    10201 cggtgtcggt tttgggcggc gtgtccgttt tcgcagcgcc cgccgtggag ggaggcgtgc
    10261 ccgcgctttt gctttccgac gggcgggtga tggtaggcgg ggtgtatttc ttcgcgggag
    10321 ttgcggcacc ggtcttggtt gaggatgccg ggcttttcga tttggaggtc acgggtgggg
    10381 actgcggctg tgccggccgg gtttgggctg gcgttccctg cgcgggcggg cgctgctgct
    10441 gcgcaggggg tgtctcgtaa cgcggagtct gcggctgcgc gtactggggt tgctgatact
    10501 gaggcggcgc gtactgcggc tggtagtatt gcggttgagc ctgctggtat tgccggggct
    10561 ggctttgata ataacccgga gcctggtcca ggctcctgcc tccatattgc ggatagcctc
    10621 cctgttgata gccggagggc ggcttgtccc cataaaacag gcggcggaac atgtcgccaa
    10681 acttgcgtcc gaaggtgggc aggaattcca tgggactgac atactccgtc cgcggaacag
    10741 gctggctgta ttgaggctgc tgctgctggg gatacgcagg cggcgcggag tagcccggag
    10801 gcctttgata ctgcggcgcg ggttggtagg gctgctgggc cggggtctgg ccgggtgcga
    10861 tgagaacgcc aaatccggct gctgccagca aggtcgtcag ggggtaggtc ttcatgttgt
    10921 taagctctct taagttttcg ggggcatcaa gccttttgtt tgcgctcatg caggagcgcc
    10981 tgccggccat gcctgattgg ggggggattt gtgacgcggc gggcgctggg aatgttcagc
    11041 cgtcttgcaa aaggattcag cgtccgcgcc gccccatcca ggcccgggct aggtaggagc
    11101 acaaaatcca gcctttgcgc agggggtgca ggcggcagcg tcgcgtgaag acttggtagc
    11161 gctcgcgtct gagtttctcc aaaatctcgc tgtaaatgcg cgccatcatg gcggcgggca
    11221 gcagatgccg ccggtcgtcg gcgggcagca ttttttcggc gtccttgaaa agtttcgcgg
    11281 cgcgctcgtg ctcgaagtcc atcaacctga caaacctctg gttatgccgg ccgtcgagga
    11341 tttccgactc ggtgacggag aactttctca agtcctccag cggcaggtag atgcggcctg
    11401 tctcgcgcgc gtcctgcccc acgtcgcgca tgatgttggt gagttggagc gcgtagccga
    11461 gcttgatggc gtagtcgcgc gaggcggggt tctggcagcc gaagatttca gcgctaacga
    11521 ggccgaccac ggacgccact ttgtagcagt atgcgagcag ctcctcatag gtctcgtaac
    11581 gaacgcgtcc gatgtcgctg gccactccgt caatgatctc cgccagccag gcgcgcggga
    11641 aaccgtattt gcgcggcagc acgaccacct catcaagcac gggatggccc ggcgcgcggc
    11701 cctcgctgac gcagcgtttc cattcatgaa gccggccctc cttttcggct tcgctgagat
    11761 gggggctgtc ggcgatgtcg tccaccacgc ggcagaaggc gtaaaaggcg atcatgtcat
    11821 gccgccgctg cgcgggcaga caggcaagcg cgagggcgag attggacttc gccttgcggg
    11881 cgatctgtgc cggagtgagc gcggtgtcgg acatgaaaag cgtgtgtgcc gccggaatgt
    11941 gaatgacact gcgaaggccg ctgtgaacaa tctggcgctg tgtctaaaaa gagccaaagc
    12001 acgcttctgg caagctccaa tgagacggcg cggcgcatga tgaggccgga ggaaaaaggt
    12061 gccgcgcgcg ggtgttcgca aggtgttcac gagttgttca ccgggtcatg aacaagcctg
    12121 cggacgaagc ttttaaaaat tctggaatga cgcggaacgt tccacatcgg agttttaaaa
    12181 acatgacaag attttcttgt gaatgatggc gttttcgcca tacaagtgag cagattttaa
    12241 aaacaaattc cggcgctgaa ttcactctcg aatcagcgcc aaatttgtcc cgattcccga
    12301 acgcatcccg aagcctcaac accaccccta tggagaccaa tcccgatgaa tgtcctcccg
    12361 ctccaaacca ccactcccac aacggcgcag cttcgacgca agagcttttc gatgtagtgc
    12421 ccggcggctg ggaccgcatc tgcgaaatcc tccaccgcaa actcggcgag gacaatttca
    12481 gccgctgttt cgccggcacc agcgcgcgcg tcaccggagg cggcaggctc gtgatttccg
    12541 tgccgaatcc gattcatcaa ttgtggatcg agagcaattt tcagggcgtg ctcaatgacg
    12601 ccgccgccga ggtgctcggc accgccgcgc gcatcgagtt cagcgtggcg ctggaaagcg
    12661 cgcagccttt ctccaaggac gcggcgccgc aggatgtgaa ggccgcgcgc gtggtcggca
    12721 cgcgccaggg cgagccgcgc aaccaggcgc cgtccggcat cccggagccg gtgtcggaga
    12781 gggcgctgat tgaggcgggg ctgaatccga agtacaactt cgacagtttc gtggtcggcg
    12841 ccaactgcag ctactccgcc gccgtggcgc gcgccgtcgc ggaaaagccg ggccgcattt
    12901 acaacccgct cttcctgcac ggcgccaccg gactcggcaa gacgcatctc atgcaggcga
    12961 tcggccagga ggtgctcatg cgcaaaaaac gcgccgtggt gcgctacgtc acctccgagc
    13021 agttcaccaa cgagtttgtc gaggccatca agaagcagaa cttcaccctc ttccgccaga
    13081 aataccgcaa ggtggacctc ctgctcattg acgacgtgca tttcttcgga ggcaaggaca
    13141 gcacgcagga ggagttcttc cacaccttca acgagctgtt caacaacgcg cgccagatcg
    13201 tcctggccag cgaccgtccg ccggcggaaa tgaagaatct cgaagcccgt ctcgtgtccc
    13261 gtttcgactg ggggctgacc acgcagatcc agacaccaga tctcgagacg cgcaccgcca
    13321 tcctccggca caaggtggcc ggcttcaacg tgacactggc gccctggatc atcgagttca
    13381 tcgcccagcg cgtgcgcacc aacgtgcgca aactggaggg cgccctcatg cgcgtcgctt
    13441 cgcgcgtgtc tttggaagga gacattccaa acgaggtcgc cctcgccgcc ttcctcgacg
    13501 acgtgatcga ggacgagccg gccaagagcg tcaccgtgga ccgggtgcag cgcgcggtgg
    13561 ccacacagta tgacctgcgt gtcagcgatc tcaccggccc gcgccgtccg aaaaacatcg
    13621 ccgaggcgcg ccaggtggcg atgtatctca cgcgccagat ggtcaaactg ccgctcattc
    13681 aaattggcga ggaattcggc gggcgcgacc acggcacggt cattcatgcg tgcaaagtcg
    13741 tgtcaaagcg catgcaccag aaggatgact tccgcatcat ggtggaccgc ctcagcgcga
    13801 agctgctgga ggcgtgagtt gaacacgaag cgggaagccg cggatgttgg tccgcagttt
    13861 tccggcagtg gtggtcgggc atgaaccaag gggattcaat aattgcgggc gtaactctgc
    13921 gggtcattca tcgcgagagc ggcctggagc gtcctgcacc tgcatgggga ccgcatggtg
    13981 cggcgcgcct tcgtccccgg cgggcgctcc accctccagc tcaaggacgt gcgcctcatc
    14041 tgcgagctgg cggagtccct cggcttctgc tcccccactc tcgaaaactg ccgcgcgcag
    14101 tgggagacct tcgtccatga aaaaggcggc ggcgacctgg accactccgg tttcttccgc
    14161 atgtctgagt gagcgtagcc gcaggcgcaa gcccgtggcc tccgcgcctg tagctgccgc
    14221 cgcctgtagc ttccgccgca aggcggtgga gtgcggaggg aaggggaccc cacagctcga
    14281 gcggggcctg aaaatcaccc gtttgcgcaa gtgactgaca ttcagtcggg aaaatgcgct
    14341 ccctggctct ccaaagcgtg tttttgaagg ttgggaggcc atttttcatg ctttggaggc
    14401 gcgtctccac gctttggtga cgcaccacca cgccgtccga gagacgctga gaagttcgga
    14461 ggcgcaccac cacgccgtcc gagagacgcc gggaagtttg gaggcgcacc tccacgccgt
    14521 ccgagagacg ccgggaagtc tggaggcgca tctccacgcc gtccgaggga cgctgagaag
    14581 ttcggaggcg cacctccacg ccgtccgagg gacgctgaga agttcggagg cgcacctcca
    14641 cgccgccgag ggacgccggg aagtttggag gttgacctcc acgccgtccg agagacgctg
    14701 agaagtctga aagttcacct ccgcgccgtc caaaatccgc tgacctgccg tctccaccgc
    14761 ctgcggagtc ctgatgacgg cggctcggtc atgattccat atccaaacaa ccagagtcag
    14821 ccgcacgctc atgatcgagg ctctcattga aatcttcttc gagttcggtc tccagatcgt
    14881 catcgagctg ttcgccgaat ggggcacccg cagcactgtc cgccggcgcg agggcggccc
    14941 gcctcctgcc tggctggccg gtgtgggtta cttcgtcctg ggcgggctgc tcggcggggc
    15001 cagcctgctt gtttttcccg cgcattgggt gacggatccc ggcctgcgcc tggtgaattt
    15061 gctcgtcact ccggtcatcg ccggactgct catgtccgcc ctcggcgcgt ggcggcgcag
    15121 ccgggggcag gagttgctgc ggctggacaa gttcacccac ggctggctgt tcgccctcgc
    15181 cctggcgctg gtgcggcact tcggagcgcg ggccgcctga ggccgcggct ttttttcttg
    15241 caggccgggg acgcgcgcat ttagcctccg cccatgccgc gtaaattcat cttcctgctc
    15301 gccctcgcca ccagcatgac cgccagcgcg caggagatgc gcgtcttcac caacagccag
    15361 ggggtggcca tcaaagccgc gctcgtcagc gtggccggcg gccaggccac catccgccgg
    15421 gaggatggcg cgcaattcac cgtggccgtc accgccttca gcgcggcgga ccaggaatac
    15481 atccgccagt ggaaacccgc cgccgcgcct tccgcaccgt ccgcgaggcc ctccgcgccc
    15541 gggccgaacg acgctctgga cgccgccgcg ctcaatgccc tggccggtca tgagcttttc
    15601 ggcgacagcc cgctctggga cgcgccggcg gaggacgcgg cgaagcgcct cggactcgac
    15661 cgcgaatcgc agacctccac gcagagcagc taccgctcct atccgccgga cgactaccgc
    15721 ctgtttggcg cgcggcccca ttccgtggcg ctctacgccg aggatggcaa aacagccagc
    15781 ctctccctcg tctatgccaa caaaggcgac ctgttcagcg cgcgcggcgg cagcgaggat
    15841 cacttcgaca aggacacgcc acccgccgag gcggcgaaga tcgtcaaaaa agccatggac
    15901 gccgatttga aaaccatcgc cgaaaccctc agcgccaaac tcggcgagcc gaagaaagag
    15961 cgcttcggcg agggcaaggc cgggcgcatg aacatgcagc gctgggactg gcgcggccac
    16021 tccatcctgc tggccgaggc cgagggcgaa tatgtgggcg tgcaggttgt gcccgccgcg
    16081 tttgccgacg cgggcggcaa gtccgtgcgt gtttccgacg cggccatccg cgagcgggca
    16141 aaggccaacg tgcagacccg cccgaacggc gacgtggtca tccaggacat ccccatggtg
    16201 gaccaggggc cgaaaggcta ctgcgtgccc gccaccgccg agcgcgccat gcgctacctg
    16261 ggcgtggccg ccgacatgta tgtgctggcc aacgccggcg agaccggcta cggcg
//
LOCUS       DAOMYJ010000113        16282 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Prosthecobacter sp. isolate AMR_MDS_5629
            NODE_1234_length_16282_cov_20.022555, whole genome shotgun
            sequence.
ACCESSION   DAOMYJ010000113 DAOMYJ010000000
VERSION     DAOMYJ010000113.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867850
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prosthecobacter sp. (wastewater metagenome)
  ORGANISM  Prosthecobacter sp.
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Verrucomicrobiaceae; Prosthecobacter.
REFERENCE   1  (bases 1 to 16282)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16282)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.30
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:34:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,742
            CDSs (total)                      :: 4,684
            Genes (coding)                    :: 4,676
            CDSs (with protein)               :: 4,676
            Genes (RNA)                       :: 58
            tRNAs                             :: 55
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16282
                     /organism="Prosthecobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1234_length_16282_cov_20.022555"
                     /isolate="AMR_MDS_5629"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1965333"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..349)
                     /locus_tag="PLP58_12625"
     CDS             complement(<1..349)
                     /locus_tag="PLP58_12625"
                     /inference="COORDINATES: protein motif:HMM:NF025713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="abortive infection family protein"
                     /protein_id="HRJ09413.1"
                     /translation="MLEAVLDELELKLSGEATEHHGELSRQYKSVAKQLGIDAGRKDL
                     DDAFKKVCTGLVNVVDGLATVRNKMSDAHARKTKPSPHHALVIANASRTVASFLIESY
                     LYQRSRGKLSGGSR"
     gene            complement(565..1827)
                     /locus_tag="PLP58_12630"
     CDS             complement(565..1827)
                     /locus_tag="PLP58_12630"
                     /inference="COORDINATES: protein motif:HMM:NF019222.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1552 domain-containing protein"
                     /protein_id="HRJ09414.1"
                     /translation="MSTFTFQKHLSRRAMLRGAGISLGLPLLEAMTPAFAAVKETKQA
                     KRFVGMSLALGLHNPNLVPEGSGRTYKPSRYLAGIQDLREDFTVVSGSSHPGVTGGHT
                     AEGSIFSACPNARGSTTRNTISLDQLMAKHLGHETRFPSLVLNTNSQTSPSYTENGTM
                     IPAENSVMKLFTRLFVNDTPAEQERQAELIRQGRSVMDVVGAEAKSLQRELGAGDRDK
                     LDAWFTSVRDLEQRLAANEAWTHKPKPKVALKPPTQIPRDNEVEVDRIFLDIVHMALA
                     TDSTRFITLHVTGNNVQGIEGVDESYHTLSHHGMDADKLEQLALVEQGVINTWAAFLR
                     KLKTDKLLDDTMVLLTSNLGNASSHDNKNMPVLFAGGGFKHGQHLAFDQKDNYPLPNL
                     YLSMLHRLGMQDESFATSTGGMRGLEAA"
     gene            1982..5305
                     /locus_tag="PLP58_12635"
     CDS             1982..5305
                     /locus_tag="PLP58_12635"
                     /inference="COORDINATES: protein motif:HMM:NF012493.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-related protein"
                     /protein_id="HRJ09415.1"
                     /translation="MTTRFFTNSSENTLLKKFSGIFEHNPDIERFDALIGYLRASGYF
                     AIRPHLDKVPQIRLLVGINVDEIMEQYHQKGRLFLADAGRAMREFRDGLSSDIAAARY
                     TLDVEKGILQFAGDVASKKIEIRAHPTKRLHAKIYLFIPKGFNEHKPGSVITGSSNLT
                     AAGLGVDEVGSNYEFNVLSHDYQDVKFASDEFEKLWAEGVHVLPKVVSDITQKSHLRD
                     DLSPFELYIKLLIEFFGPAIEYDPNSETDLPEGFMRLAYQMDAVKQGFLMLQRHHGFF
                     LSDVVGLGKTIIAVLIAKKFFYHNGFPAHLSRVLVVAPPALLDGWQRTLDKFDLQYVE
                     LHSSGSLHKIRQPDRFDLVIVDEAHKFRNDTAESYDELQRICKTPTRRRLKDNLLARK
                     KVILVSATPLNNRPNDIRNLVGLFQDLKDSTLEVSNLQHFFARRQKEYEQALKAPTIQ
                     ATRHEVARIYEEVRNRVVKPLTIRRTRTDLTESELYASDLDAQGIRFPAVKGPHKLLY
                     RLSPALEVLYDNTLSALSSGLTYNRYRAIGHLTQEKKHKYQNADRLSSQLAQIMKVLL
                     LKRLDSSFHAFTQSLMRFQQATWAMVKMFASGKIFIAPALNVSEYIVEEREDELEAKI
                     LELQESDPTLEICKPDEFDPTFLPGLKADLEKLNALVEAWKEVDEDPKLDEFLVRLKT
                     DLLNPSINHAAKVKAGSPRLVVFSESKETTNYLKVELAKHGFKSVLTIDSSNRKEQMT
                     VVRANFDANVPLEAKTKGGEQQADDYQIIISTEVLAEGVNLHRANVIVNYDTPWNSTR
                     LMQRIGRVNRIGSTASQVHVFNFFPTAQVDEGIELQRKAIAKLQAFHSALGEDSQIYS
                     TEEEVTNFGLFEAAELIEDRDETLAYLDELRKFRSSNPDRFREIRNLPLRARVGRKDR
                     HRSGHTLTYIRNHRRDAFYYSNSGEVPEELSFVEAARIFKAVAGEKAVPLHEQHHDHV
                     NAAIAQFKETLQQEAAIGQVVDHQMGPNESKALRFLAVFINHPTLATDEEKFLLRAAQ
                     AAIKKATFARLQRDLNALEKAHKKTPLQASTLLDKTLEILRRFPLQDNVDEQVEDARG
                     GLSTAELAPSIILSESFAL"
     gene            complement(5376..5513)
                     /locus_tag="PLP58_12640"
     CDS             complement(5376..5513)
                     /locus_tag="PLP58_12640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ09416.1"
                     /translation="MSACAVVGLGLLKTKLTVALLTGAVCFGTERLNNGFDGMEGITI
                     L"
     gene            5944..6396
                     /locus_tag="PLP58_12645"
     CDS             5944..6396
                     /locus_tag="PLP58_12645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007415703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HRJ09417.1"
                     /translation="MPQSLANLYIHLIFSTKERFPYLTAEVRPDLHAYMATVLANLNS
                     PAVLINSVADHVHVLFNMGRTVTLAQAVEDVKKSSSKWIKTQGASFATFAWQAGYGGF
                     SVSESNVPKVANYIRNQEEHHRVKTFQEEYREFLAKHNIQYDERYVWD"
     gene            6666..6905
                     /locus_tag="PLP58_12650"
     CDS             6666..6905
                     /locus_tag="PLP58_12650"
                     /inference="COORDINATES: protein motif:HMM:NF015630.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0175 family protein"
                     /protein_id="HRJ09418.1"
                     /translation="MTITLPDMPGLASLGEPAILLDLACGAYAAGHLSRQSAADVAGL
                     SRFDFDEALYARRISNFTEEALAQDLETFRVLSLR"
     gene            6942..7214
                     /locus_tag="PLP58_12655"
     CDS             6942..7214
                     /locus_tag="PLP58_12655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ09419.1"
                     /translation="MSIQQIPVAEFCEHAMDALQSVEKNHVLVELVRDGQIIAYLSPA
                     PQPSGNTGTLADWMGTGAGYTLAAGESLDDPTFAAEEWEDFPESGQ"
     gene            7233..7628
                     /locus_tag="PLP58_12660"
     CDS             7233..7628
                     /locus_tag="PLP58_12660"
                     /inference="COORDINATES: protein motif:HMM:NF013970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HRJ09420.1"
                     /translation="MTYLLDTNAWLHWFHRPAEMSAAARQMLNGERTVALSPMSIVEV
                     AQKHHKGRLGLPVPLDQWVRQSLPAGRVRLLPLTAEIALRAYQWPDDFHGDPADRIIA
                     ATAVLHRLTLVTSDEKLIARPDVTTLSTR"
     gene            7632..11894
                     /locus_tag="PLP58_12665"
     CDS             7632..11894
                     /locus_tag="PLP58_12665"
                     /inference="COORDINATES: protein motif:HMM:NF024352.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TaqI-like C-terminal specificity
                     domain-containing protein"
                     /protein_id="HRJ09421.1"
                     /translation="MPAPIALQSQLRLPYSRDTYQAILTDLFPSGSLHLLQHPEHIAA
                     PCEFVTRTQQLGSVQLSDGNQIALLEVEVEDQVQLARNRVALRNFVARFIDEAATQAV
                     LAVFHQPGKTDWRLTYASKRSQLDEDTLEITSIQTAPRRFTFLLGESEPCRTAAGRLA
                     EIRERGEHLTLEDVEKAFSVERLNKEFFKLYKEHYDRFVAHLLAPERVKAVRTAFGVP
                     TLRDDDEASTAAAQDKADKPIRDFVKRLLGRLVFLHFLQKKGWMGCPVGVQPSGAQGK
                     SAKAYTPAWTGGDTDFLHTYYQLAKAKGEAGQFHSRWLLPLFFEALNQDHDERPGHLF
                     APTGTRIPYLNGGLFEASEVEQTLQCRLARQSEGERPLAAKAPHSGECAYDTSLLDFP
                     AAHFEALLDFFGQYHFTIDENDPEDHEVGIDPEMLGHIFENLLEDNKDKGAYYTPKAI
                     VSYMARQSLLHYLQTHLGEHPELVSLLNEKDLTRLEKNGFVHTHAKRIAELLDAVKVC
                     DPAIGSGAFPIGVLQEILWTRLALDWTLNTPEDRARLKRQIIQNSIHGVDLDPGAVEI
                     ARLRFWLALVVDEDQPRPLPNLDYKIHRADSLIEYIRGEPVHLGKVSAEDKRTHGAIQ
                     SLIVAKQSLFTAQRVPEKRAARFALYRALAEVAIAEFTWLRNEMGLIANDAERASQLD
                     SGLKEFTYRLKLIDSAAKESAKIQDRILADLRAWFDDEKKPTFLWQLHFGEVFAQGGF
                     DIVIANPPYVRHETISDSAPLLRKRFSVAASRADLLIFFYEQSVNLLKPGGTLTFITS
                     NKYFRAGYGSKLRPFLSSKLRLHSLIDFGDAPVFDAIAYASILIGSRAAPASGHDISV
                     CNWPQDAPIHQLLTVLNQNAFAVAQKSLDPNGWSLEKRDSLALLAKLRASGKPLADYV
                     GGRFYYGIKTGLNEAFVIDEATRDRLIKEDKRSAQLIKPFLRGRDVKRWRVDHQKLYL
                     IAFQFGFHDQLKGYPAILRHLNQFESELKARGQCTTSRGNSEEGQHHWLELDNNPKEE
                     YLACFDGPKIVVPAIERNAPFAVDTGGHYSNDKTSIAVTQEPHTLCAILNSAPLWWII
                     KQVAASKQNGYFEFKPMYVRPLPIPPASAADKARLSELAQACAAAAQRGDDETLAVHE
                     AEIDQIVYRLFDLTDEEVALIESSLAPSQARKPVLKGTHGLPKKKEALFRRLRGVESA
                     YFTIDTAERIAAETMPEISAGTLKQYLSEGMEQGHFHDAGRGWYSRLPVAARLTAEPV
                     QKWLDVIRSSFPLLDFTLWSTAHWNPWLRHLISRPTVFLHCDPVNLPVLHEHLVAQGG
                     DFRRNPTVAEIAKNPLGPETVVLRPLLKGSPLGENGLASVEHCLVETWNENRAFHLLD
                     AEELRQATLRWLSSERVQVPVLLRYASSRGLGREWFGDLFTN"
     gene            11918..13042
                     /locus_tag="PLP58_12670"
     CDS             11918..13042
                     /locus_tag="PLP58_12670"
                     /inference="COORDINATES: protein motif:HMM:NF020419.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="HRJ09422.1"
                     /translation="MSAPPRFIEPAHYAQERLREACNQLQISQLPLLETCLHAFELLG
                     RLACSGLPFLFKGGTSLLLHSPVFRRISTDIDIVTPVAGDELLGLLKAVGQGAPFVGF
                     EEQDRGLRGQPQRRHFRYLYQPVMGGPPQPLLLDVVEDDFATLTVEEKLVVQPWFQPQ
                     REALVKVQTVETLLGDKLAAFAPRTTGVPYRKLRPDGSEVDGDLLQIAKQFYDVATLY
                     DLSPMPHTCLSAWLEHVKRESRYRETSFTPEAVLRDTFQACLGITMMNFPPKRRHQDT
                     AQLTQGINALQNHVISGSLSHTSLFEMAGKVAHLTTLLYASSDILAEKPKPPSDVLEL
                     KDLSISGDLRFLNPIKGTSPRGLFYWSKAAEHAPVEWIKS"
     gene            13027..13230
                     /locus_tag="PLP58_12675"
     CDS             13027..13230
                     /locus_tag="PLP58_12675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ09423.1"
                     /translation="MDQKLMAAIHQNGRLWHTRDEAIRLFTRWLGFRRTGSLIEETAR
                     SLINGLLREGSLEKNGPDEIRRA"
     gene            complement(13231..15564)
                     /locus_tag="PLP58_12680"
     CDS             complement(13231..15564)
                     /locus_tag="PLP58_12680"
                     /inference="COORDINATES: protein motif:HMM:NF019259.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1592 domain-containing protein"
                     /protein_id="HRJ09424.1"
                     /translation="MPVSFPQGARLALLSAAAPLAGAALPGPLAIFLDQHCMECHDAD
                     VKKGGLDLAALPFDLKDAAAFEKWRHVFERVHSGEMPPKKRERPPHADLEGFLSGLRH
                     PLLEADQADIAANGRVRSRRLTRVEYEHTMHDLLGGDIPLKTLLPEDRASHGFETVAD
                     GQQLSHHQLARYLDVADLALDDAFDRAMKGDATYAKHHTPEMLLKNRGGNYRGPDLRN
                     GESITWPVTLQFFGRTPTWAPASGWYRVTLRGVRGINPTSDGAVWGTLRSGHCESNAP
                     LLYMIGLVEATATPRDMTFEAWIEEGHRLELKPNDGTHKRAPTGAKGGNVSFAGRNLE
                     KEGYPGIAHRGIDIERIYPVANAEGVRKKLFGGDGLEALKAGNLDKLVHRFAARAFRR
                     PVTEAQSAPYRQIAAKSLAEGDSVPDALRAAYRAILCSPRFLSFIEAPGPLDDHAIAS
                     RLSFALWCSMPDQELLELANQNKLHEPATLAQQVERLLSHAKARRFIASFTDQWLKLN
                     QIDFTTPDTRLFRDFDSVVQESMLQETRAYFTELLRGDLSITNLIDSDFAFLNGRLAR
                     HYQADLDLKSGEGLQKVSLKAEAKRGGLVTQGAILKVTADGTSTSPVVRGVFINERIL
                     GNHIPPPPPGVPAIEPDIRGATSIRDQLDKHRNNESCASCHQTIDPPGFALENYDPVG
                     IWRKGYGKDGRGAKVDPSGLTPEGEPFTDLAAWQQIQTKRPEPLARGFATHFLTYATG
                     APPRFSDEAALDDIAKKATNIRSIIREVVLSEVFRRK"
     gene            complement(15632..15913)
                     /locus_tag="PLP58_12685"
     CDS             complement(15632..15913)
                     /locus_tag="PLP58_12685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ09425.1"
                     /translation="MTKNQIHEWHDRLEDGRRRYVRGYWNSREWTFRVLLPESDDWER
                     VERPDKDLWLALRDVLFRKYQRKRLPFKHLASVDAVLEEMGVPVEKAAE"
     gene            15948..>16282
                     /locus_tag="PLP58_12690"
     CDS             15948..>16282
                     /locus_tag="PLP58_12690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ09426.1"
                     /translation="MQAVPPPAAPAAPAALPRLLLYLAAVMAGGALLAWPLFALGKGF
                     ASWLPGSSWADFGPALWLADTAQRAHFPRYFNRAVLVCALVFMWPLLRSLRVDGALLP
                     AWRPVAAGIR"
BASE COUNT         3134 a         5225 c         4720 g         3203 t
ORIGIN      
        1 cacgtgaacc gcccgacagc ttcccgcgcg agcgttggta gaggtacgac tcgatcagga
       61 acgaagccac cgtccggctg gcgttcgcga tcacaagggc gtgatgtgga gaaggcttgg
      121 tcttgcgggc gtgagcgtcg ctcatcttgt tgcgaacggt ggcgaggccg tcgacgacgt
      181 tgaccaggcc ggtgcacacc ttcttgaacg cgtcgtcgag gtccttccgg ccagcgtcga
      241 tgcccagctg tttggcgacc gacttgtact gcctgctcag ctcgccgtga tgttccgtgg
      301 cctcgccgga gagcttgagc tcgagctcgt cgagtacagc ctccagcatg cgcgcggctg
      361 cacggcgcgg acgaccgcct cggcagcatc gaaccaggaa aagacgcctc ccttttcgct
      421 gccaccggcg acatcctcga catccgcagc caggtcaggc atctctggat cagcggtgtc
      481 gagcaaccgc tcgacacccg ccacacacgg cttgccgaga aataccgctc acgtccgaaa
      541 ctggaataga cggcgcctgc ccgctcaggc cgcttcgaga cctctcatcc cgcccgtgct
      601 tgtggcgaag ctctcatcct gcatgccgag acggtgcagc atgctcaaat agaggttggg
      661 cagcggatag ttgtcctttt gatcgaaagc gaggtgctgg ccgtgtttga agccgccgcc
      721 ggcgaagagg acgggcatgt tcttgttgtc gtggctgctg gcgttgccga ggttcgaggt
      781 gagcagcacc atcgtgtcgt cgaggagctt gtcggtcttc agtttgcgca agaaggcggc
      841 ccaggtgttg atcacgccct gctcgacgag ggcgagctgc tccagcttgt ccgcgtccat
      901 gccgtgatgg ctgagggtgt ggtagctctc atcgacgcct tcgatgccct gcacgttgtt
      961 gcccgtgacg tgcagcgtga tgaagcgcgt gctgtccgtg gccagcgcca tgtgtacgat
     1021 gtcgagaaag atgcggtcca cctccacctc gttgtcacgc gggatctgcg tcggcggctt
     1081 cagggcgacc ttcggcttcg gtttgtgcgt ccaggcctcg ttcgcggcca gccgctgctc
     1141 caggtcacgc acactggtga accatgcgtc cagcttgtct cggtcccctg ccccgagctc
     1201 acgctggagg gatttcgcct ccgcgccgac cacatccatc acgctgcggc cctggcggat
     1261 cagctccgcc tgccgctcct gctcggcggg cgtgtcgttg acgaaaagcc gtgtgaagag
     1321 tttcatcacg ctgttctccg ccgggatcat cgtgccgttc tccgtgtagc tcgggcttgt
     1381 ctgcgagttc gtgttcaaaa cgagcgatgg aaaccgcgtc tcatgcccca aatgcttcgc
     1441 catgagctgg tcgagcgaga tcgtgttccg cgtcgtcgag ccacgcgcat taggacaggc
     1501 cgaaaaaatg cttccctccg ccgtgtgacc gcccgtgacc cccggatgcg atgaacccga
     1561 caccaccgtg aaatcctccc gcagatcctg aatacccgcg agataacgcg aaggcttgta
     1621 agtcctcccc gagccctccg gcacgagatt cggattgtgc agccccaacg ccaagctcat
     1681 gccgacgaag cgctttgcct gcttcgtctc cttcaccgcc gcaaacgccg gagtcatcgc
     1741 ctccaacaac ggaaggccga gagaaatgcc cgctccgcgc agcatggcac ggcgggagag
     1801 gtgtttttga aaggtgaagg tgctcattgg ggggcgagaa ggcaacgagc ccaacagaac
     1861 agtctttccg cattgttccc gcaacatcgc gcacataaca cagcactcac aggcttgccc
     1921 ccttgccatc aatccttctc cctgcttgga tgaagcaatc ccggcctgaa tcgtttcccg
     1981 aatgaccacc cgtttcttca ccaactccag cgaaaacacc ctgctcaaga agttctcggg
     2041 catctttgag cacaatccgg acatcgagcg gtttgatgct ttgatcggct atcttcgcgc
     2101 ctccggttac ttcgccattc gaccgcatct cgacaaagtt ccgcagatcc gcctgctcgt
     2161 gggaatcaac gtggatgaga tcatggagca gtatcatcaa aaggggcgtt tgtttctggc
     2221 cgatgctgga cgtgccatgc gggagtttcg tgatggtttg tcgtcggaca tcgccgccgc
     2281 gcgttacacg ctggatgtgg agaagggcat tcttcagttt gccggggatg tggcgtcgaa
     2341 gaagatcgag attcgcgctc atcccacgaa gcgcctacac gccaaaatct acctcttcat
     2401 ccccaagggc ttcaacgaac acaaacccgg ctccgtcatc accggttcct ccaatctgac
     2461 cgctgcaggg ctcggggtgg atgaagtggg cagcaactac gagttcaacg tcctttccca
     2521 cgactatcag gacgtgaagt tcgcttccga tgagtttgag aagctttggg ccgaaggtgt
     2581 ccatgtgctt ccgaaggttg tctccgacat cacgcaaaag agccacctgc gagatgatct
     2641 ctcgcccttc gaactctaca tcaagctgct catcgagttc ttcggaccgg cgattgagta
     2701 tgatccgaac agtgagaccg acctgcccga gggcttcatg cggcttgcct atcagatgga
     2761 cgcggtgaag cagggcttct tgatgctcca acgtcatcat gggttcttcc tgtccgacgt
     2821 ggtcggtctc ggaaagacca tcatcgcggt gctcatcgcc aagaagttct tttatcacaa
     2881 cggcttcccc gcgcatctct cccgcgtgct cgtcgtggcc ccgcccgctc tgctggacgg
     2941 ctggcagcgc acgctggaca agttcgactt gcagtatgtc gaactgcaca gcagcggcag
     3001 ccttcacaaa atccgtcagc cggaccgctt cgacctcgtc atcgtggatg aggcgcacaa
     3061 gttccgaaac gacaccgccg aatcctacga cgagcttcag cgcatctgca agacgccgac
     3121 acgccgtcgc ttgaaggaca atctactagc ccgcaagaag gtcatcctcg tgtccgccac
     3181 accgctgaac aaccggccca atgacatccg caatctggtc ggcctgttcc aagacctcaa
     3241 agactccacg ttggaggtga gcaatcttca gcacttcttt gctcgtcggc agaaggaata
     3301 tgagcaagcc ttgaaggcac ccaccatcca ggcgacgcga catgaggtgg cacgtattta
     3361 tgaagaggtt cgtaatcgtg tggtgaagcc ccttaccatc cggcgcaccc gcacagacct
     3421 cacagagagc gagctttacg cttctgactt ggatgcccaa ggcatccgct tcccagccgt
     3481 caaaggtccg cacaagctgc tctaccgtct ctctccagcc ctggaagtgc tttatgataa
     3541 cacactgtcc gcactcagtt ccgggctcac ctacaaccgc taccgcgcga tcggccacct
     3601 gactcaagag aagaagcaca agtatcaaaa cgctgaccgc ctctcctccc agcttgcaca
     3661 aatcatgaag gtgctgcttt tgaagcggct cgacagcagc ttccacgcct ttacccagtc
     3721 cctcatgcgg ttccagcaag ccacctgggc gatggtgaag atgttcgcat ccgggaaaat
     3781 cttcatcgcc ccggccttga acgtctccga gtacattgtt gaggagcgcg aggatgaact
     3841 ggaggctaaa atcctcgagt tgcaggaatc ggaccctacc ctggaaatct gcaagcctga
     3901 tgagtttgat cccaccttcc ttcctggcct caaggctgat ctggagaagc tcaacgcact
     3961 cgtagaggcg tggaaggagg tcgacgagga tcccaagctc gacgagttcc tcgtgcggtt
     4021 gaaaaccgat ctgctcaatc cctccatcaa ccacgcggcc aaggtcaaag ccggctcgcc
     4081 ccgcctcgtc gtcttttccg agtcgaaaga aacaacgaac tacctgaagg tcgagttggc
     4141 gaagcatggc ttcaaaagtg tgctgacaat cgactcctcg aaccgaaagg aacagatgac
     4201 cgtcgtgcgg gcaaatttcg atgcgaatgt gcccttggaa gcaaagacga aaggcggaga
     4261 gcagcaggcg gacgactacc agatcatcat ctcgacagaa gtgctcgccg aaggcgtgaa
     4321 cctgcatcgg gcgaatgtga tcgtgaatta cgacacaccc tggaactcca cccgccttat
     4381 gcagcgcatt ggccgtgtga accgcatcgg cagcaccgcc tcgcaggtcc atgtcttcaa
     4441 tttcttcccc accgcgcagg tggatgaggg catcgaacta cagcgcaagg ccatcgccaa
     4501 gctccaggcc tttcactccg cccttggcga ggacagccag atttactcca ccgaggaaga
     4561 ggtcaccaat ttcggcctgt tcgaggcggc ggaactcatc gaggatcggg acgaaacctt
     4621 ggcatatctg gatgaactgc gaaaattccg ttccagcaac cctgaccgct tccgggagat
     4681 tcggaacctg ccgctgcgcg cgcgtgtggg gcgcaaggac cgccatcgca gcggccacac
     4741 tctcacctac attcgcaatc accgccggga tgccttttac tattccaaca gcggcgaagt
     4801 gccggaggag ctgagctttg tggaggccgc tcgcattttc aaagcagtgg caggagagaa
     4861 agccgttccg cttcatgagc agcatcacga tcatgtgaac gctgccatcg cccaattcaa
     4921 agagaccctc cagcaagaag ccgccatcgg gcaggtggtg gatcatcaga tggggccgaa
     4981 cgagtccaag gccctgcgct tcctcgccgt cttcatcaat catcccaccc tcgccaccga
     5041 tgaggagaag ttccttcttc gcgccgcgca ggcagcgatc aagaaagcca ccttcgcccg
     5101 gctccagcgc gacttgaatg ccttggaaaa ggcccacaag aagacgcccc tccaagcatc
     5161 gacgctgctc gataagacgc tcgaaatcct ccgccggttc ccgcttcagg acaatgtgga
     5221 tgagcaggtc gaggatgcgc ggggcggcct ttccaccgcc gagctcgccc cttccatcat
     5281 cctgtcggaa tccttcgctc tatgaggcta tgaatttgcc cacaccaggc cccgagggat
     5341 tcgagaggtt gctttcccaa gccctcgaac tacgttcaca atatcgttat gccctccatc
     5401 ccgtcgaagc cattgttcag cctttccgtg ccgaagcaga cagcccctgt cagcaaggcc
     5461 acggtgagct tcgttttgag caggccgagg cccactaccg cgcaggcgct caagggttac
     5521 gactatctga aatcccacag ctcctgcggc agctccggga gttaagccgt ctgtccacag
     5581 tggaccgtgc ggggcgcaag gccctgcgcc agtgggcgca gtctctttct aagcgccaac
     5641 ggcgcggtct atcgcagccc agggcaacgc cctgggacag gatgttcgaa aaagcccgga
     5701 gccctgaaag ggcggcctaa ttccaaggca ccgagcgccc aaggtgcggt tgatcgtgtc
     5761 acaacatccg gtaaattgga tcgcccattc agggctacga tctgttcgcc tcgtcatccc
     5821 agggcgttgc cctgggctac gataagccgc gccgttggcg ctgagaatct gtgcgccaaa
     5881 ggtgcaaact atcgtagcga agggcaacgc cctggctgaa agggcggcct aactgctgac
     5941 gccatgccgc aatccctcgc caatctttac atccacctga tcttctccac gaaggaacgg
     6001 tttccctatc tgacggcgga ggtgcgccct gaccttcacg cctacatggc gacggtgttg
     6061 gcaaacctga actcccccgc cgtgctcatc aactcggtgg ccgaccatgt gcatgtcctt
     6121 ttcaacatgg gccgcacggt cactttggcg caagcagtgg aagacgtgaa gaagtcgtcc
     6181 tccaaatgga tcaagacgca gggcgcgtcg tttgccacct ttgcatggca ggcgggttac
     6241 ggtgggtttt ccgtgagcga gtccaacgtg ccgaaggtgg cgaactacat ccgtaatcag
     6301 gaggaacatc atcgggtgaa gacctttcag gaggagtacc gggagttcct cgccaaacac
     6361 aacattcaat atgatgaacg ctatgtgtgg gattaggccg ccccttcagg gctccgggct
     6421 gtttgccccg caaacccagg gcgttgccct gggctgcgat agcgcgtccc tttgggactc
     6481 catgaacaag atgtccacat tcgaccatct cgactggcag cccctcatcg ccgtctggag
     6541 agatcgcttg cggcgctttg atccccttcg tcattctcca ttgctcattc gtcatttaaa
     6601 acccacgcgg acgccattcc gccttccagc ttggcgcttc gtcttctttt tgctaacctc
     6661 tccccatgac catcaccctt ccagacatgc ccggcctcgc ttccttgggt gagcctgcca
     6721 tccttctcga tctcgcctgt ggtgcttatg ccgctggcca cctttcccgc caatcggcag
     6781 cggatgtggc aggcctgagc cggttcgatt tcgatgaggc tctgtatgcc cggcgcatct
     6841 cgaacttcac ggaggaggcg ctggcccagg atttggaaac ctttcgtgtg ctcagcctcc
     6901 gttgattgac gttttctgcc cgtcccacta ttctctcaac catgagcatc caacaaattc
     6961 cagtcgccga gttttgcgag cacgcgatgg acgccctgca atccgtcgag aagaaccatg
     7021 tgctcgtcga gcttgtgcgt gatggacaga tcatcgccta cctgtccccc gcgcctcagc
     7081 cgagcggcaa caccggcacc ctggcggact ggatgggcac cggcgcgggc tacaccctgg
     7141 ctgcgggcga aagcctcgat gatcccacct tcgccgccga ggagtgggag gatttccctg
     7201 aatctggcca gtgattcgag ccgcggctcc ccatgaccta cctcctcgac acgaatgcct
     7261 ggctgcactg gtttcatcgc ccggcggaga tgtccgctgc ggcacggcag atgctgaatg
     7321 gtgagaggac ggtcgccttg tcgcccatga gcatcgtgga ggtggcgcaa aaacatcaca
     7381 aaggcaggct tggcctcccg gtgccgctgg atcagtgggt caggcaatcc ctgccagcag
     7441 gccgtgtccg cctccttccc ctcaccgctg agatcgctct gcgggcttac cagtggccgg
     7501 atgatttcca tggcgatcct gccgaccgca tcatcgcggc cacggcggtg ctgcaccggc
     7561 tgactctcgt cacctcggat gaaaagctca tcgcccgccc ggatgtgacc acgctttcca
     7621 cccgttgatt catgcccgct cccatcgctc tccagtccca gctccgtctg ccttattcca
     7681 gggacaccta tcaggccatc ctcaccgatc ttttcccttc cggcagcctg catctccttc
     7741 agcatccgga gcacatcgcc gccccgtgtg agttcgtcac ccgcacccag cagctcggca
     7801 gcgtccagct ttcagacggt aatcaaatcg cgctcctgga ggtcgaggtc gaagatcagg
     7861 tccagctcgc ccgcaaccgc gtcgccctac ggaacttcgt cgcgaggttc atcgacgaag
     7921 ccgccaccca ggccgtgctg gccgtcttcc accagccggg aaagacggac tggcggctca
     7981 cctacgcttc gaagcgctcc cagctcgatg aggacacgct ggagatcacc agcatccaga
     8041 ccgccccgcg ccgcttcacc ttccttctcg gcgaaagcga gccctgccgc accgccgcgg
     8101 gccgtctcgc ggaaatccgt gagcgtggcg agcacctcac gctggaggac gtggagaagg
     8161 ccttctccgt cgagcgcctc aacaaggagt tcttcaagct ctacaaagag cactacgacc
     8221 gcttcgtggc ccacctgctc gctcccgagc gtgtgaaggc cgtgcgcacc gccttcggcg
     8281 tgcccaccct tcgcgatgat gatgaggcat cgaccgccgc cgctcaggac aaggccgaca
     8341 aaccgatccg cgacttcgtg aagcgtctgc tcggccgcct cgtcttcctg cacttccttc
     8401 aaaagaaggg ctggatgggc tgccccgttg gcgtccagcc ttcaggcgct cagggaaaat
     8461 cggctaaagc ctatacacct gcctggaccg gcggtgacac cgatttcctc cacacctact
     8521 accagctcgc caaggccaaa ggcgaggccg ggcagttcca ctctcgctgg ctcctgccgc
     8581 tcttctttga ggcgctgaac caagaccacg acgaacgccc cggccacctc ttcgccccca
     8641 ccggcacccg catcccgtat ctgaatggcg gtctctttga ggcttctgaa gtggagcaga
     8701 cgcttcaatg taggctcgct cgccagagcg agggagaaag gccgctcgct gccaaggcac
     8761 cgcattctgg cgaatgcgcc tacgatacct ctctcctcga cttccccgcc gctcactttg
     8821 aagccctgct cgacttcttc ggccaatacc acttcaccat cgacgagaac gaccccgagg
     8881 accacgaggt cggcattgat cccgagatgc tgggccacat ctttgaaaac ctcctggagg
     8941 acaacaagga caagggcgcc tactacaccc cgaaggccat cgtctcctac atggcccgcc
     9001 agagcctgct gcactacctg caaacccacc tcggcgagca cccggagctg gtgagccttc
     9061 tcaatgagaa agacctcacc cgcctcgaaa agaacggctt cgtccacacc catgccaagc
     9121 gcatcgccga gctgctggat gccgtgaagg tctgtgatcc cgccatcggc tccggggcct
     9181 tccccatcgg cgtgcttcag gagatcctct ggacccgcct cgcactcgac tggacgctga
     9241 acacgcccga ggatcgcgcc cggctgaaac gccagatcat ccaaaacagc atccacggcg
     9301 tcgatctcga ccccggtgcc gtcgagatcg cccgcctgcg cttctggctc gcccttgtcg
     9361 tcgatgaaga ccagccccgc ccgctgccca atctcgacta caagatccat cgcgccgaca
     9421 gcctcatcga atacatccgt ggagagcctg tgcatctcgg caaagtcagc gccgaggaca
     9481 aacgcaccca cggggccatc cagagcctga tcgttgccaa acaaagcctt ttcaccgctc
     9541 agcgcgtgcc ggaaaagcgt gccgcccgct tcgcgctata ccgagccctc gcagaggtcg
     9601 ccatcgccga gttcacctgg ctgcgaaacg agatgggcct catcgccaac gatgccgaac
     9661 gcgcctccca gctcgatagc ggcctgaaag agttcactta ccgcctcaaa ctcatcgaca
     9721 gcgccgcgaa ggagtccgcc aaaatccagg accgcatcct cgccgacctt cgcgcctggt
     9781 tcgatgacga gaaaaagccc accttcctct ggcagcttca ttttggcgaa gtcttcgccc
     9841 aaggcggctt cgacatcgtc atcgccaatc cgccgtatgt ccgccacgag accatcagcg
     9901 actccgctcc gctgctgcgg aagagattca gcgtcgccgc cagccgggcc gatctgctca
     9961 ttttcttcta cgagcagtcg gtgaacctgc tcaagccggg cggcacgctc accttcatca
    10021 cctccaacaa atacttccgc gccggctatg gctcgaaact tcgtccgttc ctgtcatcca
    10081 agctcaggct gcacagcctt atcgactttg gtgacgcacc tgtcttcgat gccattgcct
    10141 atgcctccat cctcatcggc agccgcgcgg ctccagcgag cggacatgac atctccgtct
    10201 gcaactggcc gcaagatgct cccattcatc aattgctcac cgttttgaat caaaacgcct
    10261 tcgctgtggc gcagaagagc ctcgatccga acggctggag cctggaaaaa cgcgattcac
    10321 tggcactact ggccaaactc agagcgagtg gcaagccgct ggccgactat gtgggagggc
    10381 gtttctatta cgggatcaaa acaggcctca atgaggcctt tgtgattgat gaggccactc
    10441 gtgaccgcct catcaaagaa gacaaaaggt ctgctcagct tatcaaaccg ttcctgcgcg
    10501 gcagggacgt gaaacgctgg cgtgtggatc accagaaact ctacctcatc gccttccaat
    10561 ttgggtttca cgaccagttg aagggctatc cagccatctt gagacatttg aaccagtttg
    10621 agtctgaact gaaggctcgt ggtcaatgca cgacttctcg cggtaatagc gaagaagggc
    10681 agcaccactg gctggaactc gataacaacc ccaaagaaga gtatttggcc tgttttgacg
    10741 ggccgaagat cgttgttcca gccattgagc ggaacgctcc gttcgctgtg gatactggag
    10801 gtcactactc aaacgacaaa acgagtatcg ctgtcactca agaaccgcac acgctctgtg
    10861 ccatcctgaa ttcagcgcct ttgtggtgga tcatcaaaca agtcgccgcc tccaaacaga
    10921 acggctactt cgagtttaag ccgatgtatg tccggccact tcccatcccg cccgccagtg
    10981 ctgccgacaa ggcacggttg agcgaactgg cgcaggcctg cgcagcggcg gcgcagcggg
    11041 gcgatgacga gacgctggcg gtgcatgagg cggagatcga ccagatcgtc tatcgcctct
    11101 ttgacctcac cgacgaggaa gtggccctca tcgagtcctc cctggctcct tctcaagccc
    11161 ggaaacccgt gctaaaaggc acgcatggat tgcccaaaaa gaaggaggcg cttttccggc
    11221 gcctgcgggg agtcgagtcc gcctacttca cgatcgatac agccgaaaga attgctgctg
    11281 agacaatgcc ggagatcagc gctggcacgc tgaagcagta tctctctgaa ggcatggaac
    11341 aggggcattt tcacgatgcg gggcgcggct ggtattcccg cctgccggtg gcggcccggc
    11401 tgaccgctga gcctgtgcag aagtggttgg atgtgatccg ctcgtccttt cccctgctgg
    11461 acttcacact ttggagcacc gcgcactgga acccttggct gcgccacctc atcagccgcc
    11521 ccaccgtctt tctgcactgt gaccccgtga acctgcccgt gctgcatgag catctggtgg
    11581 cgcagggcgg tgactttcgc cggaacccca ccgtcgccga gatcgccaaa aacccgctgg
    11641 gccctgaaac cgtggtgctg cgcccgctgc tgaagggcag cccgctgggc gaaaacgggc
    11701 tggcgagcgt ggagcactgc cttgtagaaa cttggaatga gaaccgggcc tttcacctcc
    11761 tggacgctga ggagctgcgc caggccacgc tgcggtggtt gagttccgaa agggtgcagg
    11821 tgcccgtgct gctccgctac gcatcttctc gcgggttggg gcgagagtgg tttggcgatc
    11881 tattcaccaa ctgaaaataa acgcctattt tcagataatg agcgctccgc caagattcat
    11941 cgagcccgcc cactacgccc aggaacgcct tcgggaggca tgcaaccagt tgcaaatcag
    12001 ccaactgccc ctcctggaga cctgtttgca cgcttttgag ctgctgggcc gtttggcctg
    12061 ctccgggctg ccctttttgt tcaaaggcgg cacctctctc ctgctgcaca gtcctgtctt
    12121 ccgccgcatc tccacggaca tcgacatcgt gacgccagtg gccggggatg agctgctggg
    12181 actcctgaaa gctgtcggcc agggggctcc cttcgtaggc ttcgaggagc aggacagggg
    12241 cctccgtggg cagccgcagc ggcggcactt tcgctaccta taccagccgg tgatgggcgg
    12301 acctccccaa cctctcctcc tggatgtggt ggaggatgat ttcgccaccc tgacagtgga
    12361 ggaaaagctt gtcgtgcagc cctggtttca gccacaaagg gaggcgctgg tgaaggtgca
    12421 gacggtggaa accctgttgg gagacaagct ggccgccttt gctccccgca ccactggcgt
    12481 gccctaccgc aaactccgcc ccgatggcag tgaagtcgat ggcgacctgc tccagatcgc
    12541 caagcagttc tatgatgtgg cgacgctcta cgatctgtca cccatgccgc acacttgcct
    12601 gtcggcgtgg ctggagcatg tgaagcggga aagccggtat cgcgaaactt ccttcacgcc
    12661 cgaagcggtg ctgcgagata cctttcaagc ctgtctgggc atcacgatga tgaattttcc
    12721 tccaaagcga cggcaccagg acaccgccca gctcacccaa ggcatcaacg cgctccaaaa
    12781 tcatgtcatc tcaggcagcc tgagccacac cagcctcttc gagatggccg ggaaagtggc
    12841 ccacttgacc acactgctgt atgcatcgtc tgacatcctg gctgaaaaac ccaagcctcc
    12901 cagtgatgtg ctggaactga aagacctctc catttcgggt gacctgcgat ttctcaaccc
    12961 catcaagggc acctccccgc gaggactctt ttattggagc aaggcggctg agcatgctcc
    13021 tgtggaatgg atcaaaagct gatggccgcc atccatcaaa acggccgcct ctggcacacc
    13081 cgcgacgaag ccatccgcct cttcacccgc tggctcggct tccgccgcac cggctccctg
    13141 atcgaagaaa ccgcccgctc cctcatcaac ggcctcctcc gcgaaggcag cctcgaaaag
    13201 aacggccccg acgaaatccg aagagcatga tcacttcctc cgaaacacct cgctcaaaac
    13261 cacctcgcgg atgatggagc ggatgttggt ggctttcttc gcgatgtcat cgagcgccgc
    13321 ttcatcgctg aagcgtgggg gggcgccggt ggcgtaggtg aggaagtggg tggcgaagcc
    13381 gcgggcgagt ggctcggggc gtttcgtttg gatttgctgc caggcggcga ggtcggtgaa
    13441 gggttcgcct tcgggggtga gtccgctggg atcaactttg gctccgcggc cgtctttgcc
    13501 gtagcctttg cgccagatac cgacggggtc gtagttttcg agcgcgaagc cgggcggatc
    13561 aatggtttga tggcaactgg cgcagctttc gttgttgcga tgcttgtcga gctggtcgcg
    13621 gatgctcgtg gcaccgcgaa tgtccggctc gatggcgggc acgccgggcg gtggcggcgg
    13681 gatgtgattg ccgaggatgc gctcgttgat gaagacaccg cgcacgacgg gcgaggtgct
    13741 ggtgccgtcg gcggtgactt tgaggatcgc cccctgcgtg acgaggccgc cgcgtttggc
    13801 ctcggccttg agcgagactt tttggaggcc ttcgccgctc tttaagtcga gatcggcctg
    13861 gtagtgcctc gcgagacgtc cgttcaggaa ggcgaagtcg gaatcgatga ggttcgtgat
    13921 gctcaaatca ccacgcagca gctcggtgaa gtaggcgcgg gtttcctgga gcatggattc
    13981 ctgcaccacc gagtcgaaat cgcggaacag gcgcgtgtcg ggcgtggtga agtcgatttg
    14041 attgagcttg agccactgat cggtgaagga ggcgatgaag cgtctggctt tggcgtgcga
    14101 aagcaagcgc tcgacctgct gcgcgagtgt tgccggctcg tgaagcttgt tttgattcgc
    14161 gagttcgagc agctcttgat ccggcatgga gcaccaaagg gcgaaactga gccgcgaggc
    14221 gatggcgtgg tcatccagcg gcccaggagc ctcgatgaag gacaaaaagc ggggcgagca
    14281 caagatggcg cggtaagcgg cgcggagcgc gtcaggcacc gaatcgccct cggcgagcga
    14341 tttggcggca atttggcgat acggggcgct ttgggcctcg gtgacaggcc ggcgaaaggc
    14401 gcgcgcggcg aagcgatgga cgagtttgtc gaggtttccg gctttgaggg cctccaggcc
    14461 gtcgccgccg aaaagtttct tccgcacgcc ctcggcattc gcgacgggat aaatgcgctc
    14521 aatgtctatg ccgcgatgcg cgatgccggg atagccttct ttttcgaggt tgcggcccgc
    14581 aaagctcacg ttgccgcctt tcgcgccggt gggggcgcgt ttgtgcgtgc cgtcgttcgg
    14641 tttgagttcc aggcggtggc cttcctcgat ccatgcctcg aaagtcatgt cgcgaggcgt
    14701 cgccgtggcc tcgacgaggc cgatcatgta gagcagcggc gcgttcgact cgcagtgccc
    14761 ggagcgcagc gtgccccaca ccgcgccgtc ggaggtggga ttgatgccgc gcacgccgcg
    14821 cagcgtgacg cgataccagc cgctcgccgg cgcccaggtg ggcgtgcggc caaagaattg
    14881 cagcgtgacc ggccaggtga tgctctcgcc attgcgcaga tcgggtccgc gatagttgcc
    14941 gccgcggttt ttgaggagca tctcgggcgt gtgatgcttc gcgtaggtgg catcgccttt
    15001 catcgcgcgg tcgaaagcgt catcgagagc cagatcggcc acgtcgaggt aacgcgcgag
    15061 ctggtgatgc gagagctgct gtccgtccgc gacggtttca aagccgtgcg aggcgcgatc
    15121 ctccggcagc agcgtcttga gcgggatgtc gccgccgaga aggtcgtgca tcgtgtgctc
    15181 atactcgacg cgggtgagcc tgcggctgcg cacgcgcccg tttgcggcga tgtcggcttg
    15241 atcggcctcg agcaacggat gcctgagtcc gcttaaaaag ccctccagat ccgcgtgcgg
    15301 cgggcgttcg cgctttttcg gcggcatctc gccggagtgg acgcgctcaa acacatgccg
    15361 ccatttttcg aaggctgccg cgtccttcag gtcaaaaggc agggccgcga gatcgaggcc
    15421 gccctttttc acatcggcat cgtggcactc catgcaatgc tggtcgagga aaatggcgag
    15481 cggtccgggc agcgcggcgc cagccagcgg cgcggcggct gacagcaggg cgaggcgggc
    15541 tccttgggga aaagagacgg gcatgatcga aagagggggc tgatgggaac gccagacccc
    15601 gccgtgtcta tcggcgcgcg ccgcgagccc gtcactccgc cgccttctcc accggcacgc
    15661 ccatttcctc cagcacggcg tccacggagg cgagatgctt gaagggcagg cgcttgcgct
    15721 ggtatttgcg gaacaggaca tcccgcaggg ccagccagag gtctttatcc ggcctctcca
    15781 cgcgctccca atcatcgctc tcaggcagca gcacgcggaa ggtccactcg cgggagttcc
    15841 agtaaccgcg gacgtagcgg cggcgtccgt cttcgaggcg gtcatgccat tcgtggattt
    15901 ggtttttggt cattgagagc gggcagaatg cggtggatat gcggcccatg caagccgtcc
    15961 ctcctcccgc cgctcccgcc gctcccgccg cgctgccgcg cctgctgctt tacctggcgg
    16021 cggtgatggc cggcggcgca ttgctggcct ggccgctgtt cgcgctggga aaaggctttg
    16081 cctcctggct gcccggctcg tcctgggcgg acttcggccc ggcgctgtgg ctggcggaca
    16141 cggcgcagcg cgcgcacttc ccgcgctact tcaaccgcgc cgtcctggtc tgcgcgctgg
    16201 tcttcatgtg gccgctgctc cgctccctgc gcgtggatgg cgcgctgctg ccggcttggc
    16261 ggcccgtggc ggccggcatc cg
//
LOCUS       DAOMYJ010000114        16264 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Prosthecobacter sp. isolate AMR_MDS_5629
            NODE_1237_length_16264_cov_20.223888, whole genome shotgun
            sequence.
ACCESSION   DAOMYJ010000114 DAOMYJ010000000
VERSION     DAOMYJ010000114.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867850
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prosthecobacter sp. (wastewater metagenome)
  ORGANISM  Prosthecobacter sp.
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Verrucomicrobiaceae; Prosthecobacter.
REFERENCE   1  (bases 1 to 16264)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16264)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.30
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:34:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,742
            CDSs (total)                      :: 4,684
            Genes (coding)                    :: 4,676
            CDSs (with protein)               :: 4,676
            Genes (RNA)                       :: 58
            tRNAs                             :: 55
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16264
                     /organism="Prosthecobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1237_length_16264_cov_20.223888"
                     /isolate="AMR_MDS_5629"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1965333"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(66..989)
                     /locus_tag="PLP58_12695"
     CDS             complement(66..989)
                     /locus_tag="PLP58_12695"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRJ09427.1"
                     /translation="MLRFFASRLIQGGIVILAVLCITFVLLKRAPGSPLESERNIPEH
                     IRAQKMAQLGLDQPEIVQLWRHIRGFATFQFPVSEKNGGRTVDEIILQGFPVSALVGC
                     AALVIALGLGIPMGALAAVRPNTLEDRACMMAATAGICTPSLVLGPLMALIFGLKLRW
                     FNASGWYDADDWVLPALTLGIIYSASIARLTRAGLRETLAQEFIRTARAKGASETAVV
                     FRHAFKLACLPVLNFLGPAAAGLLSGSIVTEVVFQLPGLGRHFVGSAVNRDYELAMAC
                     AAFYAVLIVGFNLLVDAIQALLNPRIGLHKA"
     gene            1131..3749
                     /locus_tag="PLP58_12700"
     CDS             1131..3749
                     /locus_tag="PLP58_12700"
                     /inference="COORDINATES: protein motif:HMM:NF012471.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004181 - metallocarboxypeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M14 family zinc carboxypeptidase"
                     /protein_id="HRJ09428.1"
                     /translation="MHLPSRLLAALSLAALVSSRAAEPEPRALEYYLPVAASNYDPAV
                     PTPQQALGFQVGEWHLHHHELVDYLGKVAAQSDRVTLTEYARSHGRRPLVYLAITAPE
                     NHARLEDIRKAHLARVESPEKDADISGAPIVVWMGYGVHGNEPSASNASVLLAYHLAA
                     ARDAQTADFLKKAVVLLEPCLNPDGFERFADWTNSHRGRHPSARRADREHQEGWPSGR
                     SNYYWFDLNRDWLPAVHPESQGRLRLFHDWQPNIVTDYHEMGNVNRSYFFQPGIAKMV
                     HPLTPKQNQALTEKMAQEHAKALDAIGSLYYTGESYDDYYIGKGSTYPDVNGSVGVLF
                     EQASSRGYHQDTEHGRLDFPFTIRNQITTSLSTLRSAHALRAEFLAYQQGFYRDALKE
                     AAASKTQAFIFSAPGDSARLEAFQTLLARHQIQSHRPAQDVKAGDRTFAAADSLVVPA
                     RQRQFRLITAMFETRTKFESNIFYDVSAWHLPSAFGLLHAGLESAPAPAAAPLVKAAG
                     ALTGVEKAYAYVFSWEPRHAPRALWRLIEAKARCWAATRAFSTPDADGKKTLWPHGSI
                     VVPVAVQDRLSSEELRQLMETIAKEDTLEVRALGTGYHGGVPDLGSPTFQPLENNGVL
                     LVTGAGAGSVTAGEIWHHFDQVWQTPLTMAEGGQLTTTLLNSFSTVILASTPFTSLPV
                     GARSALQSWVSRGGCLIAIGSSAGSLAAQDWCKIELAKAAVDTPPKDKPTQETLPYED
                     AAERRTGRELNGVIVEARYDRTHPLAWGFGPRPLIQLMRDGATFLKPASSSYLNPLQY
                     TDAPLVSGSASKENLEAMKAVTPVQVKVVDSGRVILLTDNPVFRGHWLGTEKLLANAI
                     FFGPHVSTTGGDEDEH"
     gene            complement(4034..4378)
                     /locus_tag="PLP58_12705"
     CDS             complement(4034..4378)
                     /locus_tag="PLP58_12705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ09429.1"
                     /translation="MIPPLDTCHPFEPVPAELQRLVTVRDQLLGLCRTLDAQIRRLQS
                     TAETAPKRPCAALFERPPALAHVMDEPAGRVVLPPALPEALEPEFKDTALSELNLALT
                     RAFNEVTHRAAA"
     gene            4592..5947
                     /gene="rimO"
                     /locus_tag="PLP58_12710"
     CDS             4592..5947
                     /gene="rimO"
                     /locus_tag="PLP58_12710"
                     /EC_number="2.8.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009964121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008172 - S-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0018339 -
                     peptidyl-L-beta-methylthioaspartic acid biosynthetic
                     process from peptidyl-aspartic acid [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     RimO"
                     /protein_id="HRJ09430.1"
                     /translation="MIKVGLVSLGCAKNLIDSEIMVGHLQQAGMTMTPEAELADVLII
                     NTCSFIDMAKKESVGAIHEAVDAREESKKRKRQKIIVAGCLSQRFREELPALMPEVDA
                     FIGLDQITQVAPIIQNLVGVRDHEQENLVTKGPQYIPDYDTPRFRLTPKHFAYIKIAE
                     GCNHPCSFCIIPKIRGRHRSRSQESIVKEARQLIESGVKEINLISQDTTYFGMDKWEG
                     DRPKPTSGVDSSKGESLSTLIRELNAIPGDFWIRLLYTHPAHWSDDLIAAIAESPKVA
                     RYIDMPLQHISDNMLKLMRRETDSAHIRGLLKRIRAGIPGIAIRTTFIVGFPGETDAD
                     FQELVQFIEDEKFERAGVFNYSREEGTRADKMEGHLHHMTRKSRYEKAMLAIQRNIEH
                     VNQDLLGKTLRVLVEEPGVARGEMDAPDIDTTVFVPKRLPAGQFAEVKIKDWRGYDLV
                     A"
     gene            6132..7394
                     /gene="nusA"
                     /locus_tag="PLP58_12715"
     CDS             6132..7394
                     /gene="nusA"
                     /locus_tag="PLP58_12715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009962573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor NusA"
                     /protein_id="HRJ09431.1"
                     /translation="MISELKSVFEYYDKEKGIDREKMVDALQLALLAASKKSIGPARE
                     LRIVIDPDKGTIKAWARLIVVETVSNPWEEIAIEKARRVKKDAQVGDELDLEVTPKNM
                     GRIAAQTAKQTMLQRLRMAEKENLYDEFKDRTGDVVNGTIRRFDKSDVVVDLGKFEGV
                     MPSKERVSTEDYTPGDRMRFYVKAVEKDGTRGPEIILSRAHVNFVRRLFEFEVSEIGD
                     RTVEIASIAREPGYRTKVAVHSADDKVDPVGACVGMRGARVKNIVRELNNEKVDIIKW
                     SPDPATFIREALKPVKVLTIQVAPDKSSARLTVSDEDLSKAIGRRGQNARLTTRLVGM
                     ELNIERDEHAAEVFEGQMDTAAHELETALGINLDTARKLIQGGLGDIGMLMQVELEDI
                     SDALNGDEVLAAQVHERIQSYIPKGAQP"
     gene            7473..9758
                     /gene="infB"
                     /locus_tag="PLP58_12720"
     CDS             7473..9758
                     /gene="infB"
                     /locus_tag="PLP58_12720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009962571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HRJ09432.1"
                     /translation="MPSRPSSSKSSKAAVEDPLEEEDEVAEGKPRKKADKKVLSLIEE
                     DTPKPAKRGSAKNQAVLPHIGAKPAAAPEPAAPSEPKTSEPAKKTLDDQKKAALNLFE
                     EDEKPKTKKRQAAAASTALPPISKLKESGPIQAGPVTPPAETPVPAQAPAAEEPSPEF
                     ELTEDGKKIIHLKTPIIVKDLAEKMGLRPFKIIADLITLKVFVPNAEKAIEIDVAEKV
                     CEKHGFFLEREKRGKPPPRVEEPPVREPEPLPEPKPEEKDTRELRAPIITFMGHVDHG
                     KTSLLDAVRKTQVVEGEAGGITQHIGAYSVFHEGKPITFIDTPGHAAFSAMRARGANV
                     TDIVVLIIAADDGIMPQTREALNHAKAAGVTLMVAINKCDLPAANVMRVKSQLQDMGL
                     APVDWGGEIECMEVSAKTGQGLDNLLETMALQAEVLELKADPKAPPRATVIESSMVPG
                     KGPVATVIVRQGTLKPGQPFICGPHWGKTRALINDRGEHIQAVKPGMPVEVIGFSDMP
                     HVGDELVVMDSERNAKKLSEERHEENRQKKLASTRRSTLEHLFATIEEGNKKALKLVL
                     KADAQGSVEAILGALDEIKSDKINLDVLHSDVGPVTESDVVLASASDAIVIGFNVKVE
                     NKALNVAKNDSVQIKLYSIIYELVDQVREAMSGLLDPLTREKVLGHARVKQVFKVHRG
                     YVGGSIVIDGRIDRKQRARVLRGGQPVYDGSIETLRRFQDEVPEVRNGLECGIKLQGF
                     SDYEEGDIIECYELEKFQQTL"
     gene            9822..10172
                     /gene="rbfA"
                     /locus_tag="PLP58_12725"
     CDS             9822..10172
                     /gene="rbfA"
                     /locus_tag="PLP58_12725"
                     /inference="COORDINATES: protein motif:HMM:TIGR00082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HRJ09433.1"
                     /translation="MSLRIERVGELIKREVSMILERDFAFKDCIVTVHDAEPTVDLKE
                     CFVYVGILGPKHRHADIVEQLNKNRGAIQRPLYKRVTLRSSPHLVFRLDDSVERGVRI
                     LNAIDNLPPPAGEA"
     gene            10218..11006
                     /locus_tag="PLP58_12730"
     CDS             10218..11006
                     /locus_tag="PLP58_12730"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HRJ09434.1"
                     /translation="MPAETRDWYDTPLHYDIIFDDDTPREADFLEAMWVEHGPSGPPG
                     RVLEPACGSGRLVLEMARRGWSAAGFDGNASMLEFARGRLAAAGMGALLWQDRMESFR
                     VPGRARFDLAHCLVSTFKYLLTEDHAVSCLRRVASVLKPGGIFVLGLHLTEYGRATPD
                     HERWVAERGGIHVTCNTRTWPADPRARLEAVRTRLRVRQGGREHLQETRWHFRTYSAA
                     ELHRLLRQVPELRLAACHDFTYDAAAPLRLDGSHLDAVLVLRRR"
     gene            complement(11057..12589)
                     /locus_tag="PLP58_12735"
     CDS             complement(11057..12589)
                     /locus_tag="PLP58_12735"
                     /inference="COORDINATES: protein motif:HMM:NF015585.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialate O-acetylesterase"
                     /protein_id="HRJ09435.1"
                     /translation="MTLLKPNSLAALLLSTAVPALADVTLPALFSDHMVMQADVNAPV
                     WGWADAGEEVTVSLAGHQATARTGADGRWRTNLPPMKSRAEPLTLTVEGKNKLVIQDV
                     LIGEVWLASGQSNMAFQFSRGQYAQAETEAAALPQVRMFTVKAQSTRAPQERCEGAWV
                     VATPETVGAFSAVAWFFGKELHGRLGAPVGMINSSWGGTDIAAWTSEEAQAGVPALKE
                     AMEAWKARAAAFDAAKAEAAHEKRLAAWKEAVRKAKAEGRPAPRGPRKEAHPDVSQNR
                     PANLFNGMISPLIPYALRGAIWYQGEHNCATVEKASLYATQLPLLIQDWRARWDKELA
                     FAWVQLPNFEQTAPRPLVREAMLKSLSVPNTGMAVTIDAGEANDNHPKDKKTVGGRLA
                     LWALAKVYGADVPAWSGPLPAGHEVRGGEVVLGFEHTTGGLAARGGELRGFMIAGTDK
                     VWKPGTARIEGGKVVVSSAEVAAPVAVRYSWASNPDGNLFNGAGLPASPFRTDDWEIT
                     VP"
     gene            complement(12826..15018)
                     /locus_tag="PLP58_12740"
     CDS             complement(12826..15018)
                     /locus_tag="PLP58_12740"
                     /inference="COORDINATES: protein motif:HMM:NF037959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused MFS/spermidine synthase"
                     /protein_id="HRJ09436.1"
                     /translation="MSNSPLSSRAQAGVLLGSLSLLSAFLLFQVQPVISKFILPWFGG
                     SPGVWTTCMVFFQVLLFAGYAYAHVLTRLRPRAQAVLHSALLLGAAVCLPISPDEVWK
                     PEGGEDPSARILLLLAATVGLPYFVLSSTSPLAQVWFTRAVPGGLPWRLYALSNIGSL
                     AALLTYPFVIEPRMEVMTQTRVWSVAFVFFAALSLWLAWRNGRVPESAPAPAAETAEE
                     ETRESQGLRLARALWNSAGRRLLWVLLPALASVLLLAATNHVCQDVAVVPFMWVVPLS
                     LYLLTFIICFEHERWYARPLWAVLAAAAVVAALCYDSVDSNWSFKPFEWVWWEAPWKG
                     RVWTIPFGAWMNYRVEVTVCFGAMFLTCMVCHGELARLKPEPARLTEFYLLMSAGGAL
                     GGLAVSLGAPRVFVTYLEWPLGLLAGLGLAALALAGAFLSLRRLWLGAPLALAVLGAG
                     GWGVYELASDKLRVEPRVERVRNFYGTIYIDKDWDTGLENDYVTLVHGGIIHGMQNLG
                     ADYRQEPVTYYGHHTGIGRALDSIKARPGARVGIVGLGSGTAACYAQQGQAWRFYEIN
                     PEIVRLAEKHFTYLADARTRGAEVSIHTGDARLTLEREEAQKFDVLLLDAFSGDAIPM
                     HLLTREAFDIYARHMAPEGIIVVHVTNSYLTLAPVVERQAEALGWGVTRIMTQSEGDH
                     DSTDYVMVTKNAQFLKDTPVEEPEDPEPEGIPLWTDRYHNLFQILMKE"
     gene            15175..16041
                     /locus_tag="PLP58_12745"
     CDS             15175..16041
                     /locus_tag="PLP58_12745"
                     /inference="COORDINATES: protein motif:HMM:NF012546.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HRJ09437.1"
                     /translation="MNRLLVLLITLACALSAAAQKKPDAVDEMAALLKPDRVEVYKEA
                     GGRRLEMHIFEPEKKSVPVPCYLVIHGGGWAGGEPKRMYPFAAHYARQGMVGVSLQYR
                     LYNTKTGVSVFDCVKDARSAMRWLAAHSDGLGIDPKKIIVSGGSAGGHLAASTALFDQ
                     VNDETGDAALTVTPAALILLFPVIDTSAEGYGNARIGERWRELSPAHNVRPGLPPTLT
                     FHGTGDTVTPYAGARAFHEAMLEKGNRSMLITHEGGRHGYLMSDSKLFADTMAKSDAF
                     LRELGLLPVPAK"
     gene            16051..>16264
                     /locus_tag="PLP58_12750"
     CDS             16051..>16264
                     /locus_tag="PLP58_12750"
                     /inference="COORDINATES: protein motif:HMM:NF013348.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HRJ09438.1"
                     /translation="MPDAPADSFAWHILPPDGTPLLLAFSGGRDSVAMLDILLAAGRW
                     PLVLCHFNHGLRGRESGADARFARAQA"
BASE COUNT         3023 a         5737 c         4899 g         2605 t
ORIGIN      
        1 ccaggggccg gggtgcgcgc gggcggcgtg ctccggcgcg gcgtccatgg gttgtgcgct
       61 ggggctcatg ccttgtgcag tccgatgcgg gggttgagca gcgcctggat ggcatccacc
      121 agcaggttga agccgacgat gagcacggcg taaaaggccg cgcaggccat cgcgagctcg
      181 tagtcgcggt tcacggcgga gccgacgaag tggcgtccga gtccggggag ctgaaagacg
      241 acctcggtga cgatggagcc gctgagcagc cccgccgccg ccgggccgag gaaattgagc
      301 acgggcaggc aggcgagctt gaaggcatgg cggaagacga ccgcagtctc ggaggccccc
      361 ttggcccgcg cggtgcggat gaactcctgc gccagagtct cgcgcagccc ggcgcgcgtg
      421 agcctggcga tggaggcgct gtagatgatg ccgagggtga gcgcgggcag cacccagtcg
      481 tcggcgtcat accagccgga ggcgttgaac cagcgcagct tgaggccgaa gatcagggcc
      541 atgagcggcc ccagcacgag gctgggcgtg cagatgccgg cggtggcggc catcatgcag
      601 gcgcggtcct ccagcgtgtt cgggcgcacc gccgccagcg cgcccatggg aatgcccagc
      661 cccagcgcga tgaccagcgc cgcgcacccg acgagcgcgc tgacggggaa gccctggagg
      721 atgatctcat ccacggtgcg cccgccgttt ttctcggaca cgggaaactg gaaggtggcg
      781 aagccgcgga tgtgccgcca gagctggacg atctccggct ggtcgaggcc gagctgggcc
      841 atcttttgcg cgcggatgtg ctcggggatg ttgcgctcgc tctccagggg gctgccgggc
      901 gcgcgcttga gcagcacgaa ggtgatgcag agcaccgcca ggatgacgat gcccccttgg
      961 atgagtcggc tggcgaaaaa gcggagcatc agggaaaaat ggcggccctt gcataaccgg
     1021 ggcgcgccgc gcgcgcaaga ctcggaattc gcccctccgc cgccgtgttc atgcccgcgc
     1081 ggaaaaagaa ggaaagaccg ccgccgctgt gccatttctc tccgcccgcc atgcatctcc
     1141 cgtcacgcct cctcgccgcg ctttcactcg ccgccctcgt ctcatcccgc gccgcggagc
     1201 cggagccgcg cgcgctggaa tactacctgc cggtggcggc ctcgaactac gaccccgccg
     1261 tgccaacgcc gcaacaggcg ctcggcttcc aggtcggcga gtggcatctg catcatcatg
     1321 agctggtgga ctatctcggc aaggtggccg cgcagtcgga ccgcgtgacg ctgaccgaat
     1381 acgcgcgctc ccatgggcgg cggccgctgg tttacctcgc catcaccgcg ccggagaatc
     1441 atgcccggct ggaggacatc cgcaaagccc acctcgcccg tgtcgagtcg ccggaaaaag
     1501 acgcggacat cagcggcgcg cccattgttg tctggatggg ctacggtgtg catggcaacg
     1561 agcccagcgc ctcgaacgcc tccgttttgc tagcctacca cctcgccgcc gcgcgcgacg
     1621 cgcagacggc ggattttttg aaaaaggccg tcgtgctttt ggaaccctgc ctgaaccccg
     1681 acggcttcga gcgcttcgcc gactggacca acagccaccg cggccgccac cccagcgcgc
     1741 gccgcgcgga ccgcgagcat caggagggct ggcccagcgg gcgctcgaac tactattggt
     1801 tcgaccttaa ccgcgactgg ctgcccgccg tccacccaga gtcccaggga cggctgcggc
     1861 ttttccatga ctggcagccg aacatcgtca ccgactatca tgagatgggc aatgtgaacc
     1921 gcagttactt cttccagccc ggcatcgcca agatggtgca tccgctgaca ccgaagcaaa
     1981 accaggccct gaccgaaaag atggcgcagg agcacgcgaa ggcgctcgat gccatcggct
     2041 ccctctacta cacgggcgaa agctacgacg actactacat cggcaaaggc tccacttacc
     2101 cggatgtgaa cggctccgtc ggcgtgctct tcgagcaggc cagctcgcgc ggctaccacc
     2161 aggacaccga gcacgggcgg ctggacttcc ccttcaccat ccgcaaccag atcaccacca
     2221 gcctctccac cctgcgctcc gcccacgccc tgcgcgccga gttcctcgcc taccagcagg
     2281 gtttttaccg cgacgcgctc aaggaagccg ccgcgtctaa aacgcaggcg ttcattttct
     2341 ccgcgcccgg cgactccgcc cggctggagg ccttccagac cctgctcgcg cgccatcaaa
     2401 tccagtccca ccggccggcg caggatgtga aagcgggcga ccgcaccttc gccgccgccg
     2461 actcgctggt cgtgcccgcg cgccagcgcc agttccgcct catcaccgcg atgttcgaga
     2521 cgcggacgaa gttcgagagc aacatctttt atgacgtgtc cgcctggcat ctgccctccg
     2581 cctttggcct gctccatgcc gggctggaaa gcgcgcccgc ccccgccgcc gccccgctcg
     2641 tgaaagccgc cggagccctc actggtgtgg agaaagccta cgcctacgtt ttctcctggg
     2701 agccgcgcca cgcgccgcgc gccctttggc ggctcattga ggcaaaggcc cgctgctggg
     2761 ccgccacgcg cgccttttcc acccccgatg ccgatggcaa aaagaccctc tggccgcatg
     2821 gcagcatcgt cgtgcccgtg gccgtgcagg accggctttc atccgaagag ctgcggcagc
     2881 tcatggagac catcgccaag gaagacaccc tggaggtgcg cgcgctgggc accggctacc
     2941 acggcggcgt gcccgacctc ggcagcccca cttttcagcc gctcgaaaac aacggcgtgc
     3001 tgctcgtgac aggtgccggt gccggctccg tcacagcggg cgagatctgg caccattttg
     3061 accaggtgtg gcagacaccg ctgaccatgg ccgagggcgg gcagctcacc accacgctgc
     3121 tgaacagctt ctccaccgtc atcctcgcca gcacgccctt caccagcctg cccgttggtg
     3181 ccaggagcgc gctgcagagc tgggtctcgc gcggtggctg cctcattgcc ataggctcct
     3241 ctgcaggctc cctcgccgcc caggactggt gcaagattga actcgccaaa gccgccgttg
     3301 acaccccgcc caaggacaag ccgacccagg agacgctgcc ctatgaggac gccgccgagc
     3361 gccgcaccgg gcgcgaactc aacggcgtca tcgtcgaggc ccgatacgac cgcacccacc
     3421 ctctggcatg ggggttcggc ccgcgaccgc tcatccagct catgcgggat ggcgccacct
     3481 ttctcaaacc cgcctccagc agctatctga acccgctgca atacaccgac gcccccctcg
     3541 tctcaggctc cgcctccaag gaaaacctcg aagccatgaa agccgtcacc cccgtccagg
     3601 tcaaagtggt ggacagcggc cgcgtcatcc tcctcaccga caaccccgtc ttccgcggcc
     3661 actggctcgg caccgaaaaa ctgctcgcca acgccatctt cttcggccct cacgtcagca
     3721 ccaccggcgg ggacgaggat gagcactgaa cccgcgcgcg cggctgagaa atggagtgcg
     3781 ggcactcatg tccgcagcgg gcttcttttc agcagccggg agtgtccagt ccacggcaag
     3841 ccgccaccgg tgccttgagg ggatgtcata ccgcacatca tcacgatttg tgactccctt
     3901 cctggagtgt gggagtcgtg gagtgatgga tttccagtgc cgcagcactc catgactcca
     3961 cttcggattt cacaatctgt ggtgacgcca ggtatgcgac actggcacac cccgtcatcg
     4021 ccacccgccg ggatcaggcg gcggcgcggt gggtgacttc gttgaaggcg cgggtcaggg
     4081 cgaggttgag ttcgctcaag gcggtatctt tgaactccgg ctccagcgcc tccggcagtg
     4141 ccggtggcag gacgacacgc cccgcaggct cgtccatgac atgggccagg gcgggcgggc
     4201 gctcgaacaa ggctgcgcaa ggccgctttg gcgcggtttc ggcggtgctt tggagccgtc
     4261 tgatctgcgc gtcgagtgtg cggcaaaggc caagcaattg atcccgcacg gtcacgagcc
     4321 gttggagttc ggctggaacc ggctcaaagg ggtggcaggt gtcgagtggc ggaatcatag
     4381 attttataaa tttaagaatg aaatctgtaa atttcaaaaa gatttacccc attcaccgca
     4441 tcttgtgggg ggagaccggc gtgccctttc ggcaatgggg aatggggctg cgagtgtctt
     4501 tgggagggat ggggaggcgt gttggatggc gaattccggc tcatcttctg ctttcgcacc
     4561 ccgcattctc ccgtattctc gccgcccatt tatgatcaaa gtcggtctcg tctccctcgg
     4621 ctgcgccaag aacctcattg actccgagat catggtcggc cacctccagc aggcgggcat
     4681 gacgatgacg ccggaggctg agctggcgga tgtgctcatc atcaacacct gctccttcat
     4741 tgacatggcc aagaaggaaa gcgtgggcgc gattcatgag gcggtggacg cgcgcgagga
     4801 gtcgaagaag cgcaagcggc aaaagatcat cgtggcgggc tgcctttccc agcgtttccg
     4861 tgaggagctg cccgcgctga tgcctgaggt ggatgctttt attggcctgg accagatcac
     4921 ccaggtggcg ccgatcatcc aaaacctcgt tggcgtgagg gatcacgagc aggagaacct
     4981 ggtcaccaaa ggcccgcagt acatcccgga ctacgacacg ccgcgcttcc gcctgacgcc
     5041 gaagcacttc gcctacatca agatcgccga agggtgcaac cacccgtgct cgttttgcat
     5101 catcccgaag atccgcggcc gccaccgcag ccgcagccag gagagcatcg tcaaagaggc
     5161 gcgccagctc attgaaagcg gcgtgaagga gatcaacctc atctcccagg acacgacgta
     5221 ctttggcatg gacaagtggg agggggaccg gccgaagcca acctccggcg tggacagcag
     5281 caagggcgag tcactttcca cgctgatccg cgagctgaat gccatcccgg gggatttctg
     5341 gatccgcctg ctctacacgc acccggcgca ctggagtgac gacctcatcg cggccatcgc
     5401 ggaatccccc aaggtggcgc gctacattga catgccgctc cagcacatca gtgacaacat
     5461 gctcaagctc atgcgccgtg agacggacag cgcgcacatc cgcggcctgc tgaagcgcat
     5521 ccgcgcgggc atccccggca tcgccatccg cacgaccttc atcgtgggtt tccccggcga
     5581 gacggacgcg gatttccagg agctggtgca gttcatcgag gatgagaaat tcgagcgcgc
     5641 gggggtcttc aactactccc gcgaggaggg cacgcgcgcc gacaagatgg agggccacct
     5701 gcaccacatg acgcgcaagt cgcgctatga gaaggccatg ctcgccattc agcggaacat
     5761 cgagcatgtg aaccaggacc tgctgggcaa aaccctgcgc gtgcttgtcg aggagccggg
     5821 cgtggcgcgc ggtgagatgg acgcgccgga cattgacacc accgtctttg tcccgaagag
     5881 gctgcccgca ggccagttcg ccgaggtgaa aatcaaagac tggcgcggtt acgacttggt
     5941 ggcctgactg ggaaggggag tgatgccggc gggcggatga gcgaagaatt ttctgcaaac
     6001 ggtttttcag cttgccgaaa gacttccggg aactagactg gccgttcctt aaccgggcga
     6061 agcgctaatt ttttggcagg agtggggttt tccccgctcc ttttttgtct ggatcaggct
     6121 ttgacacttt tatgattagc gaactgaaat ccgtcttcga atactacgac aaggaaaagg
     6181 gcattgaccg tgagaaaatg gttgatgccc tccagttggc gctgctggcg gcatcgaaga
     6241 aaagcatcgg ccccgcgcgt gaactgcgaa tcgtgattga ccccgacaaa ggcaccatca
     6301 aggcatgggc gcggctcatc gtggtggaga ctgtttcgaa tccctgggag gagatcgcca
     6361 tcgagaaggc gcgccgggtc aaaaaggatg cgcaggtcgg cgatgagctc gatctggagg
     6421 tcactccgaa aaacatgggc cgcatcgccg ctcaaaccgc caagcagacc atgcttcagc
     6481 gcctgcgcat ggctgagaag gaaaatctct acgacgagtt caaggaccgc accggcgatg
     6541 tcgtcaatgg caccatccgc cggttcgaca agtcggacgt ggtcgtggat ctgggcaagt
     6601 tcgagggtgt gatgccttcc aaggagcgcg tgagcaccga ggattacact cccggcgacc
     6661 ggatgcggtt ttacgtcaag gctgtcgaaa aagacggcac ccgcggtcct gagatcatcc
     6721 tctcccgcgc gcatgtgaat tttgtgcgcc ggctttttga attcgaagtc agcgagatcg
     6781 gcgaccgtac ggtcgagatc gccagcatcg cgcgcgaacc cggttaccgc accaaggtgg
     6841 ccgtccacag cgcggatgac aaggtggacc cggtcggcgc ctgcgtcggc atgcgcggcg
     6901 cgcgagtgaa gaacatcgtg cgcgagctga acaatgagaa ggtggacatc atcaaatgga
     6961 gtcccgaccc ggccaccttc atccgcgagg cgctcaagcc ggtcaaggtg ctgaccatcc
     7021 aggtcgcccc cgacaaaagc tcggcgcgcc tgaccgtcag cgatgaggac ttgtcgaagg
     7081 ccatcggccg ccgcggacag aacgcccgcc ttaccacgcg cctggtgggc atggagttga
     7141 acatcgagcg cgacgagcac gccgccgagg tctttgaagg ccagatggac accgccgccc
     7201 acgagcttga gacagccctc ggcatcaatc tcgacaccgc gcgcaaactc attcagggag
     7261 gtcttggcga catcggcatg ctcatgcagg tcgaactcga agacatctcc gacgccctca
     7321 acggcgacga agtgctcgcc gcccaggttc acgaaagaat ccagtcctac attcccaagg
     7381 gagcccagcc atgagcctcc cggtttccaa tttttcccgt caacaaacct ttttgaccgc
     7441 accgcccgcc gttcgcacgc attcaccctc tcatgcccag ccgtccctca tcctcgaaat
     7501 ccagcaaagc cgccgtcgaa gaccctcttg aggaggaaga cgaagttgcg gagggcaagc
     7561 cgcgcaaaaa ggcggataaa aaagtgctct cgctgatcga ggaagacacg cccaagcccg
     7621 cgaaacgcgg gtcggcaaaa aaccaggctg tgctgccgca catcggcgcc aagcccgccg
     7681 ccgctccgga gcctgcggct ccgtccgaac caaagacctc cgaacccgcc aaaaaaaccc
     7741 tggatgacca gaaaaaggcg gctttgaacc tgttcgagga ggacgagaaa ccgaagacga
     7801 aaaaacgcca ggcagcggcc gcgtccacag ctctcccgcc catctccaaa ctcaaggagt
     7861 ccggccccat ccaggccggt ccggtgacgc cgcccgcgga gacgccagtc cccgctcagg
     7921 ctcctgccgc cgaggagccg tccccggagt tcgaactcac cgaggatggg aaaaaaatca
     7981 tccacctcaa aacaccgatc attgtcaagg atctggcgga gaagatgggc ctgcgtcctt
     8041 tcaaaatcat cgccgacctg atcacgctca aagtcttcgt tccaaatgcc gagaaggcca
     8101 ttgagataga tgtcgcggag aaagtctgcg aaaagcacgg cttcttcctg gagcgcgaga
     8161 aacgcggcaa gcccccgccc cgcgtcgagg agccgcccgt gcgcgagcct gagccgctgc
     8221 ccgagcccaa gcccgaggaa aaagacaccc gcgagctgcg cgcccccatc atcacattca
     8281 tgggccatgt ggaccacggc aagacctccc tgctcgacgc cgtgcgcaag acccaggtcg
     8341 tcgagggcga ggccggcggc atcacccagc acatcggcgc ctacagcgtc ttccatgagg
     8401 gcaagcccat caccttcatt gacacacccg gccacgccgc cttctccgcc atgcgcgcgc
     8461 gcggcgccaa tgtcaccgac atcgtcgtgc tcatcatcgc cgctgacgac ggcatcatgc
     8521 cgcagaccag ggaggcgctg aaccacgcca aggccgccgg tgtcaccctc atggttgcca
     8581 tcaataagtg cgacctgccc gccgccaacg tcatgcgcgt caaatcccag cttcaggaca
     8641 tgggactcgc ccccgtggac tggggcggcg agatcgaatg catggaagtc tccgccaaga
     8701 ccggccaggg actcgacaac ctcctcgaaa ccatggccct ccaggccgag gtgctcgaat
     8761 tgaaggccga tccgaaggcc ccgccgcgcg ccaccgtcat cgagtcgtcc atggttcccg
     8821 gcaagggtcc cgtggccacc gtcatcgtcc gccagggcac cctcaagccc ggccagccat
     8881 tcatttgcgg tccgcactgg ggcaagaccc gcgccctcat caacgaccgc ggagagcaca
     8941 tccaggccgt caaacccggc atgcccgtcg aagtcatcgg cttcagcgac atgccccacg
     9001 tcggcgacga gctcgtcgtc atggacagcg agcgcaacgc caagaagctc agcgaggagc
     9061 gccatgagga gaaccggcag aaaaagctcg cctccacccg ccgctccact ctggagcatc
     9121 ttttcgccac catcgaggag ggcaacaaga aggccctcaa gctcgtgctc aaagccgacg
     9181 cccaggggtc cgtcgaggcc atcctcggcg cgctcgacga gatcaagagc gacaagatca
     9241 acctcgatgt cctccactcc gatgtcgggc ccgtcaccga gtccgatgtc gtgctcgcca
     9301 gcgcctccga cgccatcgtc atcggcttca atgtcaaggt ggagaacaag gccctcaatg
     9361 tcgccaagaa cgacagcgtc caaatcaagc tctactccat catttacgag ctggtggacc
     9421 aggtccgcga ggccatgtcc ggcctgctcg acccgctcac ccgcgagaaa gtgctcggcc
     9481 acgccagggt caagcaggtc ttcaaggtac accgcggcta tgtcggcggc tccatcgtca
     9541 ttgacgggcg cattgaccgc aagcagcgcg cccgcgtcct gcgcggcggc cagcctgtgt
     9601 atgacggctc catcgagacc ctccgccgct tccaggacga ggtgcccgag gtccgcaacg
     9661 gcctcgaatg cggcatcaag ctccagggat tcagcgacta cgaggagggc gacatcatcg
     9721 agtgctacga gctggagaaa ttccagcaaa ccctctaacc cccgcctggc ggtgtgccgg
     9781 tggctcccgc cccgccgccg gcggtcatcc ccatccttcc catgtcactc cgaatcgaac
     9841 gcgtcggcga gctgatcaaa cgcgaggtca gcatgatcct tgagcgcgac ttcgcgttca
     9901 aggactgcat tgtcaccgtg catgacgccg agcccaccgt ggacctcaag gaatgcttcg
     9961 tttatgtcgg catcctcggt cccaagcacc gccacgccga cattgtcgag cagctcaaca
    10021 aaaaccgcgg cgccatccaa aggccgctct acaagcgcgt caccctgcgc agctccccgc
    10081 acctcgtctt ccgccttgat gattccgtcg agcgcggcgt gcgcatcctc aacgccatag
    10141 acaacctgcc gccgcccgcc ggggaggcgt agcctggccg gcccgtttct cccgacgcgg
    10201 gtcgttcccc atccatcatg cccgccgaga cacgcgactg gtacgacacg cccctgcact
    10261 acgacatcat cttcgatgac gacacaccgc gcgaggcgga cttcctggag gcaatgtggg
    10321 tggagcacgg accttccggc ccgccagggc gtgtcctgga gcccgcctgc ggcagcggca
    10381 ggctcgtgct ggaaatggcc cgccgcggct ggagcgcggc cggctttgac ggcaatgcga
    10441 gcatgctcga gttcgcccgc ggccgcctcg ccgccgccgg gatgggcgcg cttctgtggc
    10501 aggaccgcat ggagagcttc cgtgtgccgg gacgcgcgcg ttttgacctg gcccactgcc
    10561 ttgtcagcac cttcaaatac ctgctcaccg aggatcacgc cgtctcctgc ctgcggcgcg
    10621 tggcctccgt gttaaagcct ggcggcattt ttgtgctcgg ccttcacctc accgaatacg
    10681 ggcgcgccac gcccgaccac gagcgttggg tggccgagag gggcggcatc cacgtcacct
    10741 gcaacacccg cacctggccc gctgatccac gcgcgcgtct ggaggccgtg cgcacccgtt
    10801 tgcgcgtccg ccagggcggg cgcgagcacc tccaggagac gcgctggcac ttccgcacct
    10861 acagcgccgc cgaattgcac cgtctgctgc gccaggtgcc cgagctgcgg ctcgccgcct
    10921 gccacgactt cacctatgac gccgccgcgc ctctgcggct cgacggctcc cacctggatg
    10981 ccgtgctcgt cctgcgccgc cgctgagcct ggctggcgca gacctccagg ttgccgccct
    11041 tcatcaagcc cctctctcac ggaaccgtga tttcccagtc atccgtgcgg aagggcgagg
    11101 ccggcaggcc cgcgccgttg aaaagattcc cgtccgggtt cgaggcccaa ctgtaccgca
    11161 ccgccacagg tgccgccacc tcagcgctcg aaaccaccac tttgcccccc tcgatacgcg
    11221 ccgttcccgg cttccacacc ttgtccgtgc ccgcgatcat gaagcctctc agctccccgc
    11281 cgcgagccgc cagtccgccc gtcgtgtgct caaagcccag caccacctcg cctccgcgca
    11341 cctcatgccc ggcgggcagc gggccgctcc aggcgggaac gtccgcgcca tagaccttcg
    11401 ccagcgccca aagcgccagc cggccgccca ccgtcttttt gtccttcggg tggttgtcat
    11461 tggcttcgcc ggcgtcaatc gtcaccgcca tgcccgtgtt cggcacgctc aggcttttga
    11521 gcatcgcctc gcgcaccagc gggcgcggcg cggtctgctc gaagttcggc agttgcaccc
    11581 aggcaaaggc cagctccttg tcccagcgcg cgcgccagtc ctggatcagc aaaggcagtt
    11641 gcgtggcata cagcgacgcc ttttccaccg tcgcgcagtt gtgctccccc tggtaccaga
    11701 tggccccgcg cagcgcgtag gggatgagcg gcgagatcat gccattgaag agattggcgg
    11761 ggcggttttg agacacatcg ggatgcgcct ccttgcgcgg gccgcgcggc gctggcctgc
    11821 cctccgcctt cgctttcctc accgcttcct tccaagccgc cagccgcttc tcatgcgccg
    11881 cctccgcctt ggccgcgtca aaggccgccg ccctcgcctt ccacgcctcc atcgcctcct
    11941 tgagcgccgg cactccggcc tgcgcctcct cgctcgtcca ggcggcgatg tccgtcccgc
    12001 cccaggaact gttgatcatg cccaccggcg cgcccagcct cccgtgcagc tccttgccaa
    12061 agaaccaggc cacggcggag aaagccccca ccgtctccgg cgtggccacc acccatgcgc
    12121 cctcgcaccg ctcctgcggc gcgcgtgtgg actgcgcctt cactgtgaac atccgcacct
    12181 gcggcagcgc cgccgcctcc gtttccgcct gcgcatactg gccgcggctg aactgaaacg
    12241 ccatgttgga ctgccccgag gccagccaca cctcgccgat gagcacatcc tggatcacaa
    12301 gtttgttttt gccctccact gtcagagtca gcggctccgc ccggcttttc atcggcggca
    12361 gattcgtcct ccagcggccg tccgctcccg tccgcgccgt cgcttgatga ccggcgagcg
    12421 agaccgtcac ctcctcgccc gcgtccgccc agccccagac aggagcgttc acatccgcct
    12481 gcatcaccat gtggtcggaa aaaagcgcag gcagcgtcac atccgcaagc gcgggaacgg
    12541 cggtggagag gagcagggcg gcgagtgagt tcggtttcag aagagtcatg gcaaagcgtc
    12601 gggtggagtt gaatcgagcc catgatgacg cggcaaaagc ccccgtcttg cacgcggcaa
    12661 acagtcccgg acgcgcaggc ccgtttttgc agttgcacgc cgggcgggaa tggcacgccg
    12721 tcccggcaaa atcgcacgcc tatttcccgc aaaggcatgc cgtcctggca aaacagcacg
    12781 ctgtttttcc gcgccggcag gcgtgtgacc tggtggcgcg ggccatcact ccttcatcaa
    12841 gatctggaac aggttgtggt agcggtccgt ccacagcggg atgccttccg gctccgggtc
    12901 ctctggctcc tcgacggggg tgtccttgag gaattgggcg ttctttgtca ccatcacata
    12961 gtccgtgctg tcatggtcgc cttccgactg ggtcatgatg cgcgtcacgc cccagcccag
    13021 cgcttcggcc tggcgctcca ccaccggggc cagggtcagg tagctgttgg tcacatggac
    13081 gacgatgatg ccctccggcg ccatgtgccg cgcatagata tcaaaggcct cccgcgtcag
    13141 caggtgcatc ggtatggcgt cgccgctgaa ggcgtccagc agcagcacgt cgaacttttg
    13201 cgcctcctcc cgttccaggg tcaggcgcgc gtcgcccgtg tgaatgctca cttccgcccc
    13261 gcgcgtccgc gcgtcggcca ggtaagtgaa gtgcttctcc gccaggcgga cgatctccgg
    13321 gttgatctca taaaagcgcc acgcctgccc ctgctgcgcg taacaagccg ccgtgcccga
    13381 acccaggccc acgatgccga cccgcgcgcc cggcctggcc ttgatgctgt ccagcgcccg
    13441 gccgatgccc gtgtggtgcc cgtaatacgt caccggctcc tgccggtagt cggcccccag
    13501 gttctgcatg ccgtggatga tgccgccatg caccagggtc acataatcat tctccaggcc
    13561 cgtgtcccag tccttgtcaa tgtagatggt gccgtaaaag ttccgcaccc gctccaccct
    13621 cggctccacc cgcagcttgt cagaggccag ttcgtaaaca ccccagccgc ccgcgcccag
    13681 cactgccagc gccagcggcg cgcccagcca gagccggcgc agggagagaa aagcccccgc
    13741 cagcgccagc gccgccaggc ccaggcccgc cagcaggccc agcggccact ccaggtaggt
    13801 cacgaacacc ctcggcgcgc ccaggctcac tgccagtccg cccagcgccc cgcccgccga
    13861 catcagcagg taaaactccg tcagccgcgc cggctccggc ttcagccgcg ccagctcgcc
    13921 gtggcagacc atgcaggtga gaaacatcgc cccgaaacac accgtcacct cgacccggta
    13981 gttcatccat gcgccaaagg ggatcgtcca gacacgcccc ttccacgggg cctcccacca
    14041 cacccattca aagggtttga agctccagtt cgagtccacc gagtcgtagc acagcgcagc
    14101 caccacagcc gccgccgcca gcaccgccca cagcgggcgc gcgtaccagc gctcatgctc
    14161 gaagcagatg atgaaggtca gcaggtaaag gctcagcggc accacccaca tgaagggcac
    14221 caccgccaca tcctggcaga catggttcgt cgccgccagc agcagcacgc tggccagcgc
    14281 gggcagcagc acccagagca ggcgccgtcc cgccgagttc caaagcgcgc gcgccagcct
    14341 cagcccctgg ctttcccgtg tctcctcctc ggcggtctcc gccgccgggg caggcgcgga
    14401 ctcggggacg cgcccgttcc tccacgccag ccagagggaa agcgccgcga aaaacacaaa
    14461 cgccaccgac cacacccgcg tctgcgtcat gacctccatg cgcggctcga tgacgaacgg
    14521 atatgtcagc agcgccgcca gcgagccgat gttcgagagc gcatagagcc gccacggcag
    14581 cccgcccggc accgcgcgcg tgaaccacac ctgcgccagc gggctggtgc tgctgagcac
    14641 gaaatacggc agccccaccg tcgccgccag cagcagcaaa atccgcgccg acggatcctc
    14701 gccgccctcc ggcttccaca cctcgtccgg ggagatcggc aggcacactg cggcccccag
    14761 cagaagcgcc gagtgcagca ccgcctgcgc gcgcgggcgc agccgtgtca gcacatgcgc
    14821 gtaggcatag ccggcaaaaa gcagcacctg gaaaaacacc atgcatgtcg tccacacccc
    14881 cggactgccc ccgaaccacg gcaggatgaa tttgctgatc accggctgca cttgaaacag
    14941 caggaacgcg ctcagcaggc tcagcgagcc gagcaggaca cccgcctgcg cgcgggagga
    15001 aaggggggag ttggacatgc ggcgcgcagg ctttcacttt cccggcggaa tggaaataaa
    15061 aaaatcagct tcttccctcc cgcgcatccc cgctccgtga atttgccctt gaaatcggac
    15121 cggcctccat tgtgtggaag gagccgtggc agccgccgcg ttgactttgc cgccatgaac
    15181 cgcctgcttg tcctcctcat caccctcgcc tgcgcgctgt ccgccgcggc ccagaagaaa
    15241 cccgatgccg tggatgaaat ggctgcgctg ctgaagccgg accgcgtcga agtgtacaag
    15301 gaggcgggcg gcaggcgtct ggagatgcac atcttcgagc cggagaaaaa aagcgtcccc
    15361 gtgccctgct acctcgtcat ccacggcggc ggctgggccg gcggcgagcc gaagcgcatg
    15421 tacccctttg ccgcgcatta cgcgcgccag ggcatggtgg gcgtcagcct gcaataccgc
    15481 ctctacaaca ccaagaccgg agtcagcgtc tttgactgcg tgaaggatgc gcgctccgcc
    15541 atgcgctggc tggccgccca ttcggacggg ctgggcattg acccgaaaaa gatcatcgtc
    15601 agcggcggct ccgcgggcgg gcacctcgcc gcctccaccg ccttgtttga ccaggtcaat
    15661 gacgagaccg gcgatgccgc gctcaccgtc acccccgccg ctctcatcct gcttttcccg
    15721 gtgatagaca cctccgccga aggctacggc aacgccagga tcggtgagcg ctggcgcgag
    15781 ctttcacccg cccacaacgt ccgccccggc ctgccgccca ccctcacctt ccacggcacc
    15841 ggcgacaccg tcacccccta cgccggcgcg cgcgccttcc acgaggccat gctggaaaag
    15901 ggcaaccgca gcatgctcat cacccatgag ggcggcaggc acggctacct catgtccgac
    15961 tccaaactat tcgctgacac catggccaag tctgacgcct ttttgcgcga gctgggcctg
    16021 ctgcccgtcc cggcgaagta aaaccaagcc atgcccgacg cccctgccga cagcttcgcc
    16081 tggcacatcc tgccgccgga tggcaccccg ctgctgctgg ccttctccgg cgggcgggat
    16141 tccgtggcga tgctggacat cctgctcgcc gcagggcgct ggccgctcgt gctctgccac
    16201 ttcaaccacg gcctgcgcgg gcgcgagtcc ggcgccgacg cgcgttttgc ccgcgcgcag
    16261 gcgc
//
LOCUS       DAOMYJ010000115        15630 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Prosthecobacter sp. isolate AMR_MDS_5629
            NODE_1293_length_15630_cov_22.220032, whole genome shotgun
            sequence.
ACCESSION   DAOMYJ010000115 DAOMYJ010000000
VERSION     DAOMYJ010000115.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867850
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prosthecobacter sp. (wastewater metagenome)
  ORGANISM  Prosthecobacter sp.
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Verrucomicrobiaceae; Prosthecobacter.
REFERENCE   1  (bases 1 to 15630)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15630)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.30
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:34:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,742
            CDSs (total)                      :: 4,684
            Genes (coding)                    :: 4,676
            CDSs (with protein)               :: 4,676
            Genes (RNA)                       :: 58
            tRNAs                             :: 55
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15630
                     /organism="Prosthecobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1293_length_15630_cov_22.220032"
                     /isolate="AMR_MDS_5629"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1965333"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..920)
                     /locus_tag="PLP58_12755"
     CDS             complement(<1..920)
                     /locus_tag="PLP58_12755"
                     /inference="COORDINATES: protein motif:HMM:NF025598.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4340 domain-containing protein"
                     /protein_id="HRJ09439.1"
                     /translation="MKKILILLVVLAALVVTAVVYQKQQNATLNTAATSGVKSRELLL
                     PGLDPTAVQKIRIKDSKAEVNVSINEDRKGARLAERGGYSASIERVGRVLNELREQKI
                     ASKQIVGKGAWSEIEVQPPGDNAEGVGTQVEIYGAENKLLASFVLGKQLDITGGVSST
                     SFSGGNQRFIRIPEDGDTIWVVSNTFYDIEAKPESWLDKAFMDVQKIREIAVTGPDEK
                     ENWKVGRKDESATDYVLLDAKTGEALDGPKLSVSSLLSTPTFNDVHAKDKAGELLKGA
                     TKARITTFDGFAYDVQVAKQSKDGGDKYYL"
     gene            complement(962..2929)
                     /locus_tag="PLP58_12760"
     CDS             complement(962..2929)
                     /locus_tag="PLP58_12760"
                     /inference="COORDINATES: protein motif:HMM:NF021351.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GldG family protein"
                     /protein_id="HRJ09440.1"
                     /translation="MNLNSKEGTAGITALLVIAIVVAVNFLVGGLGLFDFRLDLTEHK
                     LYTLSDGTRNILDRLNPDKPVTIRYYVTTEDRVMPPILKTHARTVQDLLLEFQKAAKG
                     KVILEKLAPNPNTEDEDRAREDDLQGMQVNAEGDNIYLGMAIQSAAQKEVLPFLNPNE
                     ETALEYNIARAVQKVSKNTKTVIGVMSAMPVMGSPMFPFQRQRGQEPWILIQRLRMDY
                     EVRDIPMTSDTIDSDINTLLVIHPADITEQAEYAIDQYLLKGGKVIAMVDPQNWIAQA
                     YSGQPNPMTGQPGGVINTASDLPNLLKAWGVTYDKGLVVADVNYRSMMQGRQNPVALK
                     LPKEAINKDERITADLQSLLMMSAGALETESRDGLAFTALATSSEASEKIDTTAAEKL
                     RRESLTNFNPSGRRQQLALMVSGKFKTAFPGGKPKGPAESGETGGPQDDPAAPKAQSE
                     PEKPADAAEKKDETPKDDGTVKESVNNEGMVVLFSDADMLYDAFCVQQDPMTGGLIAT
                     NSNLPLMLNAIEVLSGGGDLLKVRSRASTQRPFTKMDELRENVERGYRPKLKGLETEL
                     NEIAQQIGPLRVKDGALIIDPAQVKKMEELERKQTDIKKEIREIKKTQNKQIAFTESI
                     ITLLNVLAVPLFVIAVGLLLAIRRRIATAAV"
     gene            complement(2972..3730)
                     /locus_tag="PLP58_12765"
     CDS             complement(2972..3730)
                     /locus_tag="PLP58_12765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007414856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HRJ09441.1"
                     /translation="MNKRDLDNTLAIFKREFLSYFNSPVLYVIVVIYLLASMGFTFFF
                     GRFLDTDNASLTQPFFFWHPWIYIVLAPAVGMRLWSEEHRLGTFELLMTMPLSPWQAI
                     IGKFVAAALVWLIGLTLTFPVVITVYWLGSPDLGPIITGYIASYLYALGCLAVTSAAS
                     AYTRSQVVCFIVSVALCILLTLIGYPGIVDTVVRTVPASLESIVRLISYLSFMDHFFE
                     MSKGKFIFRDVLYFLSVIVTALIITHLGLRSKRA"
     gene            complement(3777..4496)
                     /locus_tag="PLP58_12770"
     CDS             complement(3777..4496)
                     /locus_tag="PLP58_12770"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HRJ09442.1"
                     /translation="MQNLRKTFGSKVAVDNVSFTVEKGQVLGFLGPNGAGKSTSMRMV
                     TGYFRPTAGSISIGGVDMLEDPETAKRSIGYLPENAPLYSDMTVASFLGFCAEMRGLN
                     GAARSRAIDRVLELCFLENVRNQSVDTLSKGYRHRTCFAQSIIHDPDVLILDEPTDGL
                     DPNQKHEVRGLIKRMGQNKAIIFSTHILEEVEAACSRAIIIDRGRVVADGTPDELKKS
                     VPGCGTLDEVFRAITRPDTVK"
     gene            5029..6438
                     /locus_tag="PLP58_12775"
     CDS             5029..6438
                     /locus_tag="PLP58_12775"
                     /inference="COORDINATES: protein motif:HMM:NF012320.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tubulin beta chain"
                     /protein_id="HRJ09443.1"
                     /translation="MKVNNTIVISIGQAGNQIAASFWKTICLEHGIDPTTGQLVQGKA
                     PAGNWSAFFSQLGESSSGSFVPRAVMVDLEPSVIQQIKATSGSLFNPANLISRTEGAG
                     GNFAVGYLGAGREVLPEVIARLDHEIGKCDNVGGVIVLHAVGGGSGSGLGALVIESIK
                     EKYPETPVLSCAILPSPQISSVVTEPYNTVFALNSLRRSADACLIFDNEALFELAHRK
                     WNIESPTVDDLNLLITEALTGLTASMRFSGFLTVEISLRELLTNLVPQPSLHFLMCAF
                     APLTPPDRSKFEEMGIEEMIKSLFDNGSVFAACSPMEGRFLSTAVLYRGIMEDKPQAD
                     AALAAMREKLPLTYWIPTAFKIGYVEQPGVSHRKSMVLLANNTEIARVLDRICHNFDK
                     LWQRKAFANWYLNEGMTEEQINALRASAQELIQSYQVAEESGAKAKVQDEGETLARPA
                     AAPAKAGGMSLRDLVDRRN"
     gene            6496..7776
                     /locus_tag="PLP58_12780"
     CDS             6496..7776
                     /locus_tag="PLP58_12780"
                     /inference="COORDINATES: protein motif:HMM:NF012320.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tubulin beta chain"
                     /protein_id="HRJ09444.1"
                     /translation="MREILSIHVGQCGNQIADRFWRLALREHGLTEAGTPREGANTAA
                     NTNMEVFFHKVRDGKYVPRAVLVDLEPGVIARIEGGDMAQLFDESSIVRKIPGAANNW
                     ARGYNVEGERVIDQIMNVIDAAAEKAPGLQGFLLTHSIGGGSGSGLGSLVLERLRQAY
                     PKKRIFTFSVAPSPLISDSAVEPYNAVLTLQRILDNADAAVLLDNEALFRIAKSKLQR
                     APNYMDLNHIIALIMSSVTASLRFPGKLNTDLSEFVTNLVPFPGNHFLTASFAPMRAP
                     GQEGQVRINFPDLARETFAQDNFTAAIDWNQGVYLAASALFRGDVRAREVDENMASIR
                     KSLNFASYMPASGGLKLGHAEVAPEGFASSGLALVNHTGIAAVFERLIKQFDIMFDNH
                     AYTHWYENAGVSRDMMAKARDTISNLAQSYRDAS"
     gene            7804..8577
                     /locus_tag="PLP58_12785"
     CDS             7804..8577
                     /locus_tag="PLP58_12785"
                     /inference="COORDINATES: protein motif:HMM:NF037660.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HRJ09445.1"
                     /translation="MDSSQIERLIATASRSVEEARRLAYHDSSKLGQLVEQISVLADL
                     RQKNGDFRKAESLYREALYRAQELRQPDPALVTGIHSLLAHLYDRWGRPDHAADYYQK
                     ALASAEAAGILDNENVAIIKNNLAMLFKQMRDYPKAERYYREALEIFRHSDGEHSPRV
                     ASVLNNLGVLYYTNMDVEHAQQMHEHALSIRESLGNDQMDPADLSQTYINLGAVYKAT
                     GDFRKAEDCVDRAKRIRAGMNGFHPNPRRAAALLVDKTL"
     gene            8574..8810
                     /locus_tag="PLP58_12790"
     CDS             8574..8810
                     /locus_tag="PLP58_12790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ09446.1"
                     /translation="MNPAFENLRQLLRRRLEIIADHALRESDPAGQLRRLQEVSEAIQ
                     AEHEKLRPALHPRLNHFLTQSSLQKALDWIEQEG"
     gene            8855..9907
                     /locus_tag="PLP58_12795"
     CDS             8855..9907
                     /locus_tag="PLP58_12795"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HRJ09447.1"
                     /translation="MTAASLTRRDTLGLLLGAPLAAAGKDAPVFIQSTRIAKAAEYSA
                     KRKGLALLVRQHGKLLHESYPNGAKSGETRRIYSGTKAFWGLAMLAAVEDGILSLDEP
                     AAAVIHEWRGKADKEKITLEQLMDFTCGLERCLRIHEDGLENRDRMAIGRPVLAAPGK
                     RFIYGPSAMQVAHQMLKRRLAGRWRNETPARYLERRVLRPLGLGPQRYIPDAAGNPLL
                     ASGFMMTPAQWARMGEVILAKGAPVLKKPASWELATEGSAANPAYSFGFWNNRKAADK
                     NAREFDIEDMLEVDWDKQGWTRACVCRDAPPDLVACIGSGYQRLFAVPSMSLVIVRQG
                     TNARFSDGDFLRLLLG"
     gene            10007..10081
                     /locus_tag="PLP58_12800"
     tRNA            10007..10081
                     /locus_tag="PLP58_12800"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:10039..10041,aa:Gly,seq:gcc)
     gene            10136..10984
                     /locus_tag="PLP58_12805"
     CDS             10136..10984
                     /locus_tag="PLP58_12805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009962471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HRJ09448.1"
                     /translation="MFVWSKLSSAKWGDAWEERFGGNPHFSLVITSVPGRPTIRVELY
                     AEKEKPLLAVQRDFGGSVREIKSRNWAALTTPVTPPLQVRGRLVICAARTPAEVAKAA
                     KEHPGREVIAIPADMAFGTGHHATTATVLRLLVDAATRLKGARWTMADLGCGSGILAI
                     AAAKLGAAKVWGCDFDPQAVKVAKENLQRNATPKVKLVRADVLKWQPKEQYDIVAANI
                     FFDILEAGFPQFVRAVKPGGILMASGILKEQAPHCLQVAAGLGIRWDKVLAKGKWVTA
                     IGTRVS"
     gene            11012..12604
                     /locus_tag="PLP58_12810"
     CDS             11012..12604
                     /locus_tag="PLP58_12810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018289905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HRJ09449.1"
                     /translation="MPDPRPKPDLMAKLRDVPHTPGVYVMRDRLHSVIYVGKARDLRK
                     RLGNYFTPARSRLADRKTRALIASIWDFDVHQVRSEPEALLLEGRLIKDFRPKYNISF
                     RDDKRFLLVRVHFQDDFPRFSLTRLKKDDGARYFGPFAHSGALRTTINWLNKKFGLRV
                     CRPLRPGEHDYKHCSNDIIKNCSAPCIGRVSREDYHKRVQQACSYLEGKGLRDLLSVL
                     EEEMQKAAARLDFEKAAELRDMVEDLRKTLSPARTFTRGARAKVVSTLDPMADVRELQ
                     DALRLERPPLVMECFDIANIGVTHCVASMVRFANGVPDNASYRRYRIRAVSGQNDFVS
                     MAEVIRRRFSRILLEGRRAMGDDADFTEETALEAMRRLEKSKFEIRDSKFVTLPDLVI
                     VDGGKGQLGMAVEELQRLGLGGLPIIGLAKENEEIHRPGESAPLILSHDTGALKLLQR
                     IRDEAHRWANGYHQLLLARRVSESLLDDCPGISQARKAALLKAFGSVARLRKASQEDI
                     AAIPGISATLAATITDFLRSRE"
     gene            12620..12907
                     /locus_tag="PLP58_12815"
     CDS             12620..12907
                     /locus_tag="PLP58_12815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ09450.1"
                     /translation="MKPTIYEQVTRSAFWTLWSLTVILALYCICDWKFQNYLSTDPRT
                     YIPETAKFLLDVTAKALLLTTLFVVRRNKIMSLCGMAGGLLWLAYLALPRY"
     gene            13053..13655
                     /locus_tag="PLP58_12820"
     CDS             13053..13655
                     /locus_tag="PLP58_12820"
                     /EC_number="1.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018970067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016646 - oxidoreductase activity, acting
                     on the CH-NH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HRJ09451.1"
                     /translation="MELDLTGPLREDAYMILAGLVTPRPIALTTTVDPEGRVNAAPFS
                     FFNVLGDSPPIVGLCPGDRAPGVPKDTARNIRLTGEFVVNLVDEALAEAMNLCAASLP
                     PGENELLHAGLTALPSSVVRPPRIAEAPASLECRRHSILEIGGNRLIIGEVLRVHVRE
                     SILDPATWLVNPAHYHPIGRMAAPHWYSRTRDMFELRRPR"
     gene            14132..14779
                     /locus_tag="PLP58_12825"
     CDS             14132..14779
                     /locus_tag="PLP58_12825"
                     /inference="COORDINATES: protein motif:HMM:NF025006.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase regulatory-like
                     domain-containing protein"
                     /protein_id="HRJ09452.1"
                     /translation="MGKACSIFAVCLLLSAAPALADGIVKGRVALDQPRPPEVKPGYK
                     PETKKPVQGEDAPRALVWLERADGAHPKTRAGEVLRIRQNGYQFRPALAAVQTGSSAE
                     FPNEDDEFHNVFSYSRARRFDLGRFRKDEPSPRITFDKPGLVKIYCEIHQHMRCRLLV
                     LDTPWFTTTAADGSFTLPKLPAGDYTLKALLPSEKTRETRLTLREGQTLTVDLAR"
     gene            14793..>15630
                     /locus_tag="PLP58_12830"
     CDS             14793..>15630
                     /locus_tag="PLP58_12830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ09453.1"
                     /translation="MTFRAKLQQTILGVVAGTTAAVLVVAQSQNSASYRALVDTMFHE
                     RMATFRQEQDSRLADAKKQAARLAESVRLFAALEEGDPETYNVAADELRLAELDFFRL
                     ASRESGLMPPPGQSRAGFLTAEQESALASALALAARAMPEEETEAHAGFVALPSANGP
                     ELYRVLAMQIRNFDTTVGTLFLGQSVARFAAPGDDGDAPGLMTLFHGEDFFIAPRVEA
                     RVLREVGVVMQSGTGEAGPGGFSFFIGGVEHKAAAHRLNEGSAFAPAWLVSVFSLADL
                     EAR"
BASE COUNT         2813 a         5222 c         4774 g         2821 t
ORIGIN      
        1 ctgaggtaat atttgtcgcc gccgtccttt gactgtttgg cgacctgcac atcataggca
       61 aagccgtcga aggtggtgat cctcgccttg gtcgcgcctt tgagcagctc gccggctttg
      121 tctttggcat ggacgtcgtt gaaggtggga gtggagagca gactgctgac ggagagcttg
      181 gggccgtcca gcgcctcgcc ggtttttgcg tcgaggagga catagtcggt ggcgctctca
      241 tctttgcgcc cgactttcca gttttccttt tcatcgggac cggtgacggc gatctcccgg
      301 atcttctgca cgtccatgaa tgccttgtcg agccaggact ccggcttggc ctcaatgtcg
      361 tagaaagtat tgctgacgac ccagatggtg tcgccatcct cggggatgcg gatgaagcgc
      421 tgattgccgc cgctgaagga ggtgctgctg acgccgccag tgatgtccaa ctgcttgccg
      481 agcacaaagg aggcgagcag cttgttttca gcgccataga tttccacctg agtgccgaca
      541 ccttcggcat tgtcgccggg aggctggact tcgatctcgg accaggcgcc ttttccgacg
      601 atctgcttgc tggcgatttt ttgctcgcgc agctcattaa gcacgcggcc gacgcgctca
      661 atgctggcgg agtaaccgcc gcgctctgcc agcctggcgc ctttgcggtc ctcgttgatg
      721 gagacgttga cctcggcctt ggagtccttg atgcggattt tctggactgc ggtggggtcg
      781 aggccgggca gcagcagttc ccggcttttc acgccgctgg tggcggcggt gttcagagtg
      841 gcgttttgct gtttttggta aaccacggcc gtgacgacga gcgcggccag cacgaccagg
      901 aggatgagga tcttcttcat gggttttgcg tgtggggtaa aaagtgcggt tcaaacgagg
      961 gtcagactgc cgcggtggcg atgcgccggc ggatggccag gaggaggccc accgcgatga
     1021 caaaaagggg aacggcgagc acgttgagca gggtgatgat ggattcggtg aaggcgatct
     1081 gcttgttctg ggtctttttg atctcgcgga tttccttttt gatgtcggtc tgcttccttt
     1141 ccagctcctc catttttttg acctgggcgg gatcaataat caaggcgccg tccttgacgc
     1201 gcagcggtcc gatctgctgg gcgatctcgt tgagctccgt ttcgaggcct ttgagcttgg
     1261 gccggtagcc ccgctccaca ttctcgcgca gttcgtccat tttggtgaag ggcctctggg
     1321 ttgaggctcg gctgcggacc ttgagaaggt ctccgccacc gctgaggact tcgatggcgt
     1381 tgagcatgag gggcaggttt gaattggtgg cgatgaggcc gcctgtcatc gggtcctgct
     1441 gcacgcagaa ggcgtcgtag agcatgtcgg cgtccgagaa caggacgacc atgccctcgt
     1501 tgttgacgga ctctttgacc gtgccgtcat ctttgggagt ctcgtccttt ttctccgccg
     1561 cgtccgcggg cttttcaggc tcgctctgag ctttgggcgc tgcgggatca tcctgggggc
     1621 cgccggtttc gccgctttca gcggggcctt tgggcttgcc gccggggaag gcggtcttga
     1681 atttgccgct gaccatgagg gcgagctgct ggcggcggcc gctgggatta aagttggtga
     1741 gcgactcccg gcgcagtttt tcggccgcgg tggtgtcaat cttctcgctg gcctcggagc
     1801 tggtggctag cgcggtgaat gcgaggccgt ccctgctctc ggtttccagc gcgccggcgg
     1861 acatcatgag cagggactgc aagtcggcgg tgatgcgctc gtccttgttg atggcctcct
     1921 tgggcagctt gagtgccacg ggattttggc ggccctgcat catggagcgg tagttcacgt
     1981 cggcgacgac gagacccttg tcataggtga ctccccaggc tttgaggagg ttgggcaggt
     2041 cggacgctgt gttgatgacg ccgccaggct gaccggtcat ggggttgggc tggccgctgt
     2101 aggcctgggc gatccagttt tgcgggtcca ccatggcaat cactttgccg cctttgagca
     2161 aatattggtc aatggcatat tcggcctgct cggtgatgtc ggcaggatgg atgacgagca
     2221 gggtgttgat gtcggagtca atggtgtcgc tggtcatggg gatgtcccgc acctcgtagt
     2281 ccatgcgcag acgctggatg aggatccacg gctcctggcc gcgctggcgc tggaagggga
     2341 acatggggga gcccatgacg ggcatggcgc tcatgacgcc gatgacggtc ttggtgtttt
     2401 tggagacctt ctggacggcg cgggcgatgt tgtattcgag cgcggtttcc tcgttggggt
     2461 tgaggaaggg aagcacctcc ttctgcgcgg cgctctggat ggccatgccg aggtagatgt
     2521 tgtcaccttc ggcgttgacc tgcatgccct ggaggtcgtc ctcgcgggcg cggtcctcat
     2581 cctcggtgtt gggattgggg gcgagctttt cgaggatgac ctttcccttg gcggcttttt
     2641 ggaactcaag gaggaggtcc tgcacggtgc gggcgtgggt tttgagaatg ggcggcatga
     2701 cgcggtcctc ggtggtgacg tagtagcgga tggtgaccgg cttgtctgga ttgaggcggt
     2761 cgaggatgtt ccgcgtgccg tcggagaggg tgtagagttt gtgctcggtg aggtcgagcc
     2821 ggaagtcgaa aaggccgagg ccgccgacga ggaagttcac ggccacgaca atggcgatga
     2881 ccagcagggc cgtgatgccc gcggtgcctt ctttgctgtt cagattcatg ttgagttcgc
     2941 ggttggggtt ctcagttcac ggatgaaaaa atcaggcgcg cttcgagcgc aggccgaggt
     3001 gggtgatgat aagggctgtg acgatgacgg agaggaagta gagcacgtcg cggaagatga
     3061 acttgccctt cgacatttcg aagaagtggt ccatgaagct caaatagctg atgagacgca
     3121 cgatgctctc cagcgaggcg gggacggtgc gcaccacggt gtcaacgatg cctgggtaac
     3181 cgatgagagt gagcaggatg cacagggcca cggaaacgat gaagcaaacc acctggctgc
     3241 gggtgtaggc gctggcggcg ctggtgacgg ccaggcagcc tagcgcgtac aagtagctgg
     3301 cgatgtagcc tgtgatgatg gggccgaggt ccgggctgcc cagccagtaa accgtgatca
     3361 ccaccgggaa ggtgagcgtg aggccgataa gccagacgag ggcggcggcg acgaatttgc
     3421 caatgatggc ctgccagggt gaaagcggca tggtcatgag cagctcgaag gtgccgagac
     3481 ggtgctcctc agaccagagc ctcatgccga cagccggggc cagcacaatg taaatccatg
     3541 gatgccagaa gaagaacggc tgggtaaggg aggcgttgtc agtgtcgagg aacctgccga
     3601 aaaagaaggt gaagcccatc gaggccagca ggtagatcac cacaatgacg tagagcacgg
     3661 gcgagttgaa gtaggagagg aactcccgct tgaagatcgc cagggtgttg tccaggtcgc
     3721 gtttgttcat gattgatttg gaatggggga aaggggttgc gctgcggggt gcggtgtcac
     3781 ttcacggtgt ccggccgggt gatggcgcgg aagacttcgt caagcgtgcc gcaaccggga
     3841 acggattttt tcaactcatc aggcgtgccg tccgcgacga cgcggccgcg gtcaatgatg
     3901 atggcgcgcg agcacgcggc ctccacctcc tccaggatgt gggtggaaaa gatgatggct
     3961 ttgttttgcc ccattcgctt gatgagaccg cgcacctcgt gcttctggtt cggatcgaga
     4021 ccgtcggtgg gttcgtcgag gatgagcaca tccgggtcgt gaatgatgct ctgggcgaag
     4081 caggttcggt ggcggtagcc tttggaaagg gtgtccacgc tctggttgcg gacgttttcc
     4141 agaaagcaaa gctccagcac gcggtctatg gccctgctcc tggcagcgcc gttcaggccg
     4201 cgcatctccg cgcagaagcc gaggaagctg gccaccgtca tgtcggagta aagcggcgcg
     4261 ttttccggca ggtaaccgat gctgcgtttc gcagtttccg ggtcttcgag catgtcaact
     4321 ccgccaatgc tgatgctccc ggcggtgggg cggaagtagc ccgtgaccat gcgcatggag
     4381 gtggatttgc cggcgccgtt ggggccaagg aagccgagca cctggccttt ctcgacagtg
     4441 aaggagacgt tgtccaccgc caccttgctg ccgaacgtct ttcggaggtt ttgcacttgg
     4501 atcatactgt cactctgagg ggttgagggt tggttggttt gggagcgccg tgtgacacag
     4561 cggcccaatt gcgcgttcaa aaaacgcgcg gcccgcttta tggtcgccgg acgcttgttt
     4621 gcaaccggat tttccgtcag ttcgccagtg ggaatgagaa ccggcgccga tgaatattgg
     4681 aagaagcgcg ctcctatcgc ttcaatgatc cagcagcccc gctctacagc agaagctcct
     4741 tggatatggg ttcatgacag tcgctttctt gtcatgatgg tcatgcggca atgacgcttc
     4801 attcagccgc caaatcgccc ggccttgccc ggcacttccc tcccttcctg tttgaaaaac
     4861 agtcaaaaaa cacatggcgg gttgaacgga aactgctgca agcactggaa ttccaaagaa
     4921 agtttcttga ttcaaagagg ccgctttgat aaatttttca aattcgagaa cttaatttgc
     4981 aaaaattctc accactgaca gattccccat aattattaaa catcccgaat gaaggtcaac
     5041 aacaccatcg tcatctctat cggccaagcc ggaaaccaaa tcgccgcatc tttttggaaa
     5101 accatctgcc tggagcatgg tattgaccca accacgggcc agcttgtcca ggggaaggcc
     5161 cccgcaggca attggagcgc ttttttctcc cagcttggcg aatcttccag cggcagtttt
     5221 gtgccgaggg cggtgatggt tgacctggag ccaagtgtca tccagcagat caaggcgacg
     5281 agcggctccc tcttcaaccc cgccaatctc atctcccgca ccgagggcgc gggcggcaat
     5341 ttcgccgtcg gctatctggg cgctggccgg gaagtattgc ctgaggtcat cgcgcgtctg
     5401 gaccacgaga tcggcaaatg cgacaacgtc ggcggtgtca tcgtgctgca tgcggtgggt
     5461 ggcggctccg gctccggtct cggcgcgctg gtcatcgagt ccatcaagga aaaatacccg
     5521 gaaacccctg tcttgagctg cgccattctg ccttcgccgc aaatttcctc ggtggtcacg
     5581 gagccctaca acacggtttt cgccctcaac agtctgcgcc gctccgccga cgcctgcctc
     5641 atcttcgaca atgaggcgct ctttgagctg gcccaccgga agtggaacat cgaaagcccc
     5701 acggtggacg atttgaacct gctcatcacc gaggcgctca ccggcctcac cgcctccatg
     5761 cgcttcagcg gcttcctcac ggtggaaatc agcctgcgcg agctgctcac caaccttgtg
     5821 ccgcagccca gcctgcactt cctcatgtgc gccttcgccc cgctgacgcc gcccgaccgc
     5881 agcaagttcg aggaaatggg catcgaggag atgatcaagt ccttgttcga caacggctcc
     5941 gtctttgccg cctgctcgcc gatggagggg cgcttcctct ccaccgccgt gctgtaccgc
     6001 ggcatcatgg aggacaaacc ccaggctgac gccgcgctcg ccgcgatgcg cgaaaaactg
     6061 ccgctcacct actggattcc caccgccttc aaaatcggct acgtcgagca gcccggcgtc
     6121 tcccaccgca agagcatggt cctgctggcg aacaacaccg agatcgcccg cgtgctcgac
     6181 cgcatctgcc acaactttga caaactctgg cagcgcaaag ccttcgccaa ctggtacctc
     6241 aacgagggca tgaccgagga gcagatcaac gccctgcgcg cctccgccca ggagctcatc
     6301 cagagctacc aggtggccga ggaaagcggc gccaaggcca aagtccagga cgagggcgag
     6361 accctcgccc gccccgccgc cgcgccagcc aaagccgggg gcatgagcct gcgcgacctc
     6421 gtggaccggc gcaactgacc cgcgaccgca tccccgcaca ccccatcatt caagaaaaac
     6481 cagaggagac acacagtgag agaaattctg agcatccacg tcggacaatg cggcaaccag
     6541 atcgccgacc gcttttggcg gctggccttg cgcgagcacg gcctcaccga ggcgggcaca
     6601 ccgagggagg gggccaacac cgccgccaac accaacatgg aggtcttttt ccacaaggtg
     6661 cgcgacggca aatacgtccc ccgcgccgtg cttgtggacc ttgagcccgg agtcatcgcc
     6721 cgcatcgagg gtggagacat ggcgcagctc tttgatgaaa gcagcatcgt ccgcaaaatc
     6781 cccggcgccg ccaacaactg ggcgcgcggc tacaacgtcg agggcgagcg cgtgattgac
     6841 cagatcatga acgtcattga cgccgccgcc gaaaaagcgc ccggcctcca gggattcctg
     6901 ctcacccact ccatcggcgg cggctccggc tccggcctcg gctccctcgt cctcgagcgc
     6961 ctgcgccagg cctatccgaa aaagcgcatc ttcaccttct ccgtggcccc ctcgccgctg
     7021 atctccgact ccgctgtcga gccctacaac gccgtgctca cccttcagcg catcctcgac
     7081 aacgccgacg ccgccgtgct gctcgacaac gaggcgctct tccgcatcgc caagtccaag
     7141 ctccagcgcg cccccaatta catggacctc aaccacatca tcgctctcat catgagctcg
     7201 gtgaccgcgt ccctgcgctt ccccggcaag ctcaacaccg acctcagcga gttcgtcacc
     7261 aacctcgtcc ccttccccgg caaccacttc ctcaccgcca gcttcgcccc catgcgcgcg
     7321 cccggccagg aggggcaggt gcgcatcaac ttccccgacc tcgcccgcga gaccttcgcc
     7381 caggacaact tcaccgccgc cattgactgg aaccagggcg tctatctcgc cgccagcgcc
     7441 ctcttccgcg gcgacgtgcg cgccagggag gtggacgaaa acatggccag catccgcaag
     7501 tccctcaact tcgccagcta catgcccgct tccggcggcc tcaagctcgg ccatgccgag
     7561 gtcgcccccg aaggcttcgc ctccagcggt ctcgcgctgg tcaaccacac cggcatcgcc
     7621 gccgtcttcg agcgcctcat caagcagttc gacatcatgt tcgacaacca cgcctacacc
     7681 cactggtatg aaaatgccgg cgtctcccgc gacatgatgg ccaaagcccg cgacaccatc
     7741 tccaacctcg cccaatccta ccgcgacgcc agttgaggcc gccgcgcttt tccctcccca
     7801 gccatggact ccagccagat cgaacggctc atcgccaccg cctcccgcag cgtcgaggag
     7861 gcgcgccgtc tggcctatca tgactcctcc aagctcggcc agctcgtcga gcagatcagc
     7921 gtcctggccg acctgcggca gaaaaacggc gacttccgca aggccgagtc cctttaccgc
     7981 gaggcccttt accgcgccca ggagctgcgc cagccggacc ccgcccttgt caccggcatc
     8041 cactccctcc tggcccacct ctacgaccgc tggggccgcc ccgaccacgc cgccgactac
     8101 taccagaaag ccctcgccag cgccgaggcc gccggcatcc tcgacaacga aaacgtcgcc
     8161 atcatcaaga acaacctcgc catgctcttc aagcagatgc gcgactaccc gaaagccgag
     8221 cgctactacc gcgaggccct cgaaatcttc cgccactccg acggcgagca cagcccccgc
     8281 gtcgccagcg tgctcaacaa cctcggcgtc ctctactaca ccaacatgga tgtcgagcac
     8341 gcccagcaga tgcacgagca cgccctcagc atccgcgaaa gccttggcaa cgaccagatg
     8401 gaccccgccg acctctccca gacctacatc aacctcggcg ccgtttacaa agccaccggc
     8461 gacttccgca aggccgagga ctgcgtggac cgcgccaagc gcatccgcgc cggcatgaac
     8521 ggcttccatc ccaatccccg ccgcgccgcc gccctgctcg ttgacaagac gctgtgaacc
     8581 cggccttcga aaacctccgc cagctcctgc gccgcaggct cgaaatcatc gccgaccatg
     8641 ccttgaggga aagcgacccc gccggacagc tccgccggct tcaagaggtt tccgaggcca
     8701 tccaggccga acacgaaaag ctccgccccg ccctgcaccc ccgcctcaac cacttcctca
     8761 cccagtccag cctgcaaaaa gcgctcgact ggattgagca ggaggggtga gcctgcccgg
     8821 ttgcgccagg ccccgacccg gcgcaccgtt ccgcatgacc gcagcaagtc tcacacgacg
     8881 cgacacactc gggctgttgc tcggcgcgcc attggctgcc gccggcaagg acgctccggt
     8941 tttcatccaa agcacccgta ttgccaaagc ggctgaatat tcggcaaaac gcaagggcct
     9001 ggccctgctc gtgcgccagc atggaaagct gctgcacgag agttatccga acggggcgaa
     9061 gtccggcgag acgcgccgca tttacagcgg caccaaggcc ttctggggcc tcgccatgct
     9121 cgccgccgtg gaggacggca ttctctcact ggatgaaccc gccgccgccg tcattcacga
     9181 gtggaggggg aaggcggaca aggaaaagat cactctcgaa cagctcatgg atttcacctg
     9241 cggcctggag cgctgcctgc gcattcacga ggacggtctg gaaaaccgcg accgcatggc
     9301 catcgggaga cccgtgctcg ccgcgcccgg caagcgcttc atctacggcc cctcggccat
     9361 gcaggtggcg caccagatgc tgaagcgccg cctcgccggt cgctggcgga atgagacgcc
     9421 cgcgcgctac ctggagcgcc gcgttttgag gccgctcggc ctcggccccc agcgctacat
     9481 ccctgacgcg gcggggaatc cgctgctggc ctccggcttc atgatgacac ccgcgcagtg
     9541 ggcgcgcatg ggggaggtca tcctggcaaa gggcgcgccg gttttgaaaa agcccgcctc
     9601 ctgggaactc gccaccgaag gctcggcggc caatccggcc tacagcttcg gcttctggaa
     9661 caaccgcaag gcggcggaca aaaacgcccg cgagttcgac atcgaggaca tgctggaggt
     9721 ggactgggac aagcagggct ggacccgcgc ctgcgtctgc cgggacgcgc cgcccgacct
     9781 cgtcgcctgc atcggcagcg gctaccagcg gctctttgcc gtcccctcca tgagcctcgt
     9841 catcgtgcgc cagggcacca acgcgcgctt ctccgacggc gacttcctcc gcctcctgct
     9901 cggataaggc gcccggtcct ccaagcgccg catcccttgg ggaaaaacgc caactttgcg
     9961 cttgcgccag cacgaagttg tttcaccatg tcgccccaac gcaaaagcgg gtgtagctca
    10021 gtggtagagc acttccttgc caaggaagat gtcgcgcgtt cgaatcgcgt cacccgctcc
    10081 aagttctcca aatcaaagga cttgccccgc atccaccacg cgcttcccat gccgcatgtt
    10141 cgtctggtcc aaactctcct ccgccaaatg gggtgacgcc tgggaagagc gcttcggcgg
    10201 caatccccat ttcagcctcg tcatcaccag cgtgcccggc aggcccacca tccgcgtcga
    10261 gctttacgcc gaaaaagaaa agcccctgct cgccgtccag cgcgacttcg gcggcagcgt
    10321 gcgcgagatc aaaagccgga actgggccgc cctcaccacg cccgtcacac cgccgctcca
    10381 ggtgcgcggc aggctcgtca tctgcgccgc gcgcaccccc gccgaggtgg caaaggccgc
    10441 taaggagcac cctggcaggg aggtcatcgc catcccggcg gacatggcct tcggcaccgg
    10501 gcaccacgcc accaccgcca ccgtgctgcg cctgctggtg gacgccgcca cccggctcaa
    10561 aggcgcgcgc tggaccatgg ccgacctcgg ctgcggcagc ggcatcctcg ccatcgccgc
    10621 cgccaagctc ggcgccgcca aagtctgggg ctgcgacttc gacccccagg ccgtgaaggt
    10681 tgccaaagag aacctccagc gcaacgccac ccccaaggtc aaactcgtcc gcgccgacgt
    10741 gctcaagtgg cagccgaagg agcaatacga catcgtcgcc gccaacatct tcttcgacat
    10801 cctcgaggcc ggcttccccc agttcgtccg cgctgtgaag cccggcggca tcctcatggc
    10861 ctccggcatc ctcaaagagc aggcccctca ttgcctccaa gtcgccgccg gcctcggcat
    10921 ccgctgggac aaagtgctcg ccaaaggcaa gtgggtcacc gccatcggca cgcgcgtatc
    10981 ctaagtttcg gaaaaacccc gccgactccc catgccagac ccgcgcccca agcccgacct
    11041 catggccaag ctgcgcgacg tgccccacac gccgggtgtc tatgtcatgc gcgaccgcct
    11101 gcacagcgtc atctacgtcg gcaaagcgcg cgacctgcgc aagcgcctcg gcaactactt
    11161 cacccccgcc cgctcccgtc tcgccgaccg caagacccgc gccctcatcg ccagcatctg
    11221 ggacttcgat gtccaccagg tccgcagcga gcccgaggcc ctcctgctcg aaggccgcct
    11281 catcaaggac ttccgcccca aatacaacat cagcttccgc gacgacaagc gcttcctcct
    11341 cgtccgcgtc cacttccagg acgacttccc ccgcttctcc ctcacccggc tgaaaaagga
    11401 cgacggcgcg cgttacttcg gccccttcgc ccactccggc gccctgcgca ccaccatcaa
    11461 ctggctcaac aaaaaattcg gcctccgcgt ctgccgcccc ctccgccccg gcgagcacga
    11521 ctacaagcac tgctccaacg acatcatcaa aaactgctcc gccccctgca tcggccgcgt
    11581 ctcccgggag gactaccaca agcgcgttca gcaggcctgc tcctacctcg aaggcaaggg
    11641 cctgcgcgac ctcctctccg tgctggagga ggagatgcag aaagccgccg cccggctcga
    11701 cttcgaaaaa gccgccgagc tgcgcgacat ggtcgaggac ctgcgcaaga ccctcagccc
    11761 cgcgcgcacc ttcacccgcg gcgcccgcgc caaagtcgtc tccaccctcg accccatggc
    11821 cgacgtgcgc gagctgcagg acgccctccg cctggagcgg cccccgctcg tcatggagtg
    11881 cttcgacatc gccaacatcg gcgtcaccca ctgcgtcgcc agcatggtcc gcttcgccaa
    11941 cggcgtgccc gacaacgcca gctaccgccg ctaccgcatc cgcgccgtca gcggccagaa
    12001 cgactttgtc agcatggccg aggtcatccg ccgccgcttc tcccgcatcc tcctggaggg
    12061 ccgccgcgcc atgggggacg acgccgactt caccgaagag accgccctcg aggccatgcg
    12121 ccggctggaa aagtcgaaat tcgaaattcg agattcaaag ttcgtcaccc tgcctgacct
    12181 cgtcatcgtg gacggcggca aaggccagct cggcatggcc gtggaggagc tgcagcgcct
    12241 cggcctcggc ggcctgccca tcatcggcct cgccaaggaa aacgaggaaa tccaccgccc
    12301 cggcgaatcc gccccgctca tcctctccca cgacacgggt gccttgaagc tgctccagcg
    12361 catccgggac gaggcccacc gctgggccaa cggctaccac cagctcctcc tggcccggcg
    12421 cgtctccgag agcctgctcg atgactgccc cggcatcagc caggcgcgca aagccgccct
    12481 cctcaaagcc ttcggctccg tcgcccgcct gcgcaaagcc agccaggaag acatcgccgc
    12541 catccccggc atcagcgcca ccctcgccgc caccatcacg gactttttgc gctccaggga
    12601 gtgagccccg acaccggtca tgaagcccac catttacgag caagtcacgc ggagcgcgtt
    12661 ttggaccctt tggtcgttga ctgtgatcct cgcgctctac tgcatctgcg actggaaatt
    12721 tcaaaactac ctgtccaccg atccgcgcac ttacattcct gagacggcca aattccttct
    12781 cgacgtcaca gcgaaagcac tcctgctgac cacgttgttt gtggtgcgtc gaaacaaaat
    12841 catgagcttg tgtggcatgg caggtggatt gctctggctg gcctatctgg ctctgccacg
    12901 gtattgaagg aatactcctg gcgaaggccg gcggccttcc gcatcaaagc gaaggctagc
    12961 ccgcccagcg ggctactctg gttcaatttc catttttcag ttttcagttt tcagttttca
    13021 gttttccatt ttccatccat tgcccattgc ccatggaact cgacctcacc ggccctctcc
    13081 gcgaagacgc ttacatgatc ctcgccggcc tcgtcacccc gcgccccatc gccctcacca
    13141 ccacggtgga cccggagggg cgggtgaatg ccgcgccgtt cagctttttc aatgtgctgg
    13201 gggattcgcc gcccatcgtc ggcctctgcc ccggcgaccg cgcgcccggc gtgcccaagg
    13261 acaccgcgcg gaacatccgc ctcacgggcg agttcgtcgt caatctcgtg gatgaggccc
    13321 tggcggaggc gatgaacctc tgcgccgcct ccctgccgcc gggggagaat gagctgctcc
    13381 acgccgggct caccgcgctg ccctccagcg tggtgcgccc gccgcgcatc gccgaggcgc
    13441 ccgccagcct ggagtgccgc cgccacagca tcctcgagat cggcgggaac cggctcatca
    13501 tcggcgaggt gctgcgcgtg catgtgcggg agagcattct cgatcccgcc acctggctgg
    13561 tgaacccggc gcattaccac cccatcggcc gcatggccgc cccgcattgg tacagccgca
    13621 cgcgggacat gttcgagctg cgccgcccgc gctgaggcgg ggaggagttc gcgttggagt
    13681 tcaagcttta gcttgtccac tgacctcccg cagcgaagcc cgctcccgtg tcgcctaaag
    13741 gcttcactcc aacgccgcaa cgggggcgcg cgcttctgtg gcacatgaag gctccacacc
    13801 gactcagcgc gccacatttc cgccaaagcg cgcttccctt tcccggcgcg cgcggtttga
    13861 atgccgggca ttatctcctt gggcacgatt gcggagtcgt gcggcttgtg gggagtctcc
    13921 cctggcacga ttgcggagtc gcgcggcagg tggggaggct gccttgacag tgaaaaagag
    13981 tcgctcggag gcagggcagg ctgccggggt ggcggaaagg tctttggcgc gggttcggga
    14041 gcttctgcac gccggccctc accgcgccgc cctccagcgg gggtgtgatc cgcccttggc
    14101 aaaacgcgcg gatgcggcag ggtcgggcgg catgggaaaa gcgtgctcca ttttcgccgt
    14161 ctgcctgctg ctaagcgcgg cgcccgcgct cgcggatggc atcgtcaaag gccgcgtggc
    14221 gctggaccag ccgcgcccgc cggaggtgaa gccgggctac aaaccggaaa cgaagaagcc
    14281 tgtgcagggt gaggacgcgc cgcgagcgct ggtgtggctg gagcgcgcgg acggggcgca
    14341 tccgaaaacg cgcgcggggg aggtgctgcg catccgccag aacggctacc agttccgccc
    14401 ggcgctggcg gcggtgcaga cggggtccag cgcggagttc ccgaacgagg atgacgagtt
    14461 ccacaatgtc ttctcctact cgcgcgcgcg ccgctttgac ctgggccgct tccgcaagga
    14521 cgagccgagc ccgcgcatca ccttcgacaa gccggggctg gtgaagattt actgcgagat
    14581 ccaccagcac atgcgctgcc gcctgctggt gctggacacg ccctggttca ccaccacggc
    14641 ggcggacggc tccttcacgc tgccgaagct gcccgcgggc gactacacac tcaaagcgct
    14701 gctgccctcg gaaaagacgc gggaaacgag gctgaccctg cgcgagggcc agacgctgac
    14761 agtggacctg gcgcgctgag aactcccgcc gcatgacctt ccgcgccaag ctccagcaga
    14821 ccatcctggg tgtcgtggcc ggcaccacgg cagccgtgct ggtggtggcg cagagccaga
    14881 acagcgcctc ctaccgcgcg ctggttgaca ccatgttcca tgagcgcatg gcgaccttcc
    14941 gccaggagca ggactcgcgg ctggcggatg cgaaaaagca ggccgcgcgg ctggcggagt
    15001 ccgtgcgcct cttcgccgcg ctggaggagg gcgatcccga aacttacaat gtggcggcgg
    15061 atgagctgcg gctggcggag ctggacttct tccgcctggc ctcccgcgaa tcgggcctga
    15121 tgccgccgcc ggggcaaagc cgcgccggat ttctcaccgc agagcaggag tcggcgctgg
    15181 cctcggctct ggccctggca gccagggcca tgccggagga ggagactgaa gcgcacgcgg
    15241 gcttcgtggc cctgccctcg gcaaacgggc ctgagcttta ccgcgtgctg gccatgcaga
    15301 tccgcaactt cgacacgacg gtgggcacgc tcttcctcgg ccagtcggtg gcgcgcttcg
    15361 ccgctccggg agacgacggg gacgctccgg gcctgatgac gctgtttcat ggcgaggatt
    15421 ttttcatcgc gccgcgcgtg gaggcgcgcg tgctgcggga ggtgggcgtg gtgatgcaat
    15481 ccgggacagg agaggcgggg ccgggaggct tttcgttttt catcgggggc gtggagcaca
    15541 aggcggcggc gcaccgtctg aacgagggct cggcttttgc cccggcatgg ctggtgagcg
    15601 tcttctccct cgcggacctg gaggcgcggc
//
LOCUS       DAOMYJ010000116        15336 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Prosthecobacter sp. isolate AMR_MDS_5629
            NODE_1319_length_15336_cov_21.668674, whole genome shotgun
            sequence.
ACCESSION   DAOMYJ010000116 DAOMYJ010000000
VERSION     DAOMYJ010000116.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867850
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prosthecobacter sp. (wastewater metagenome)
  ORGANISM  Prosthecobacter sp.
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Verrucomicrobiaceae; Prosthecobacter.
REFERENCE   1  (bases 1 to 15336)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15336)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.30
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:34:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,742
            CDSs (total)                      :: 4,684
            Genes (coding)                    :: 4,676
            CDSs (with protein)               :: 4,676
            Genes (RNA)                       :: 58
            tRNAs                             :: 55
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15336
                     /organism="Prosthecobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1319_length_15336_cov_21.668674"
                     /isolate="AMR_MDS_5629"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1965333"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2291
                     /locus_tag="PLP58_12835"
     CDS             <1..2291
                     /locus_tag="PLP58_12835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ09454.1"
                     /translation="RFPVNTALKAGAYTVRARLEAGGVSSTRALDFHIVPRLPAVRMP
                     VIMWGAGSDEIPRLKDIGFTHFIGLGAQLGEIWAQKKDAPPGDADFIARNRAALDAAL
                     AAGLGVVASVSPARLFEGKPEFHRVDREGRPFPRATICASMPELPPFFENVGRSLARA
                     HGSHPAFTTVLVNTEVRDGSRPSFNAVDRENYRAFAGADIPAEVDQRTGVDWTRLKDF
                     PVDRVVPDDHPILKYYRWFWTVGDGWNALHSALHKGVKSVSSRQWTFFDPAVRQPSIS
                     GAGGTVDVLSHWTYTYPDPQRIGLCADQLLAMSTASGRGQKVMKMTQLIWYRSQTAPV
                     KPGRPENPVAWEDQDPDAAYITIAPMHLREAFWAKIARPVQGIMYHGWQSLVPVTNSS
                     SGYRFTNPNTMHVLKELIHEVVEPLGPALMKIPDERHEVAFLESFTSQVFARRGGHGY
                     NGTWSADAWLALQHAHVPVDILFEETLLKDGLNGRRILVMTECDVLSQSVLSKIREWQ
                     AKGGKILADEHLCPALKADFVIPSFKRSKNAAEDKARVLDLAAQISGQTGVFGLSPGP
                     QADTPEVILRARRAGDARYLFAVNDRREAGSYVGQHGLVLENGLPTRAMLAWPQDAVH
                     VYDLTRARQVIPQREDEGRLRWPCELGPCDGRLYMLTPKPLLSLKLDAPDSAKPGHSA
                     TLAVTLTTTQEAPLNAVVPVEVRVRDANGRPAEGSGHYATEGGRLSVTLDIAPNEDPG
                     SWEIRVRELASGMESASWMRVE"
     gene            complement(2305..4737)
                     /locus_tag="PLP58_12840"
     CDS             complement(2305..4737)
                     /locus_tag="PLP58_12840"
                     /inference="COORDINATES: protein motif:HMM:NF014325.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-binding protein"
                     /protein_id="HRJ09455.1"
                     /translation="MEWVLPFDKTGYRDVVEADEAKFTPGAEWSFAALSQKAHEVPLP
                     PPPPPERPDKQTDEKHRQECYRQAGRHLAENADVLVALWDGIYGGGMGGTGDTLAYAV
                     SQECQQIRKQKGRTPLRVVWLAVPRQSNPHPATEAFTLGAIQPARLARFSGLAGRKLF
                     WPVTRALLLAALFFFSVFFSLAGYRESVDACERDTISMLDHSILALSHLVMEGLTEDT
                     GLGGGGAWQVRLGRWLAVGFAGLGLAAFFEKVFGVWAWLRRWFTAHFLPHDLICGLGW
                     RGRALVENGSRRRPTLVIEQQPDEAARDLCRRAGAMLIEGDAGDPAILRQAGIARLRH
                     AFVCSQDDETNMRIVHELAKLGGGGGIFFGRGKGAPLGRKTSDDQALVCGVSLKDRAT
                     AQILKDVLPENHGLDLRIFNAETVTARMLLERHPLDRFAASPDAAGARVVLIGESLMA
                     WELFEQFLQLGHFEKGRPLSIAWLSPDPQAARDRLVARHPVYSAAEREGQPCAFPEWV
                     WLEKQVLPVVLFLKHPSSERALVDLFEGGLALRAKKEVTSVFIALESAPESAALAALL
                     APVLERQRAALKRDITLTLYYNTPEDVYRDDIEQALNRDHPTLPVVVFSDFMGDCSKK
                     AVRGDEIDRVARRLNGIYAFGLGKSLGMPIEDFDGKCVDAWSGISEADKESSRQAAAH
                     AHVKARVRKRLIGKGQTAAAAEEALARIEHDRWCAYSLLKGFRPLTRIPSAVGAGFAA
                     SESEKTDILRWFEDKTFKNSCRRARIHADLVPFDDFDTLFDAEQAAKEKKKDMDQIQA
                     LNLLLNGLRAAD"
     gene            complement(5150..6256)
                     /gene="hemW"
                     /locus_tag="PLP58_12845"
     CDS             complement(5150..6256)
                     /gene="hemW"
                     /locus_tag="PLP58_12845"
                     /inference="COORDINATES: protein motif:HMM:TIGR00539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051989 - coproporphyrinogen dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM family heme chaperone HemW"
                     /protein_id="HRJ09456.1"
                     /translation="MIEHLYFHIPFCHRVCPYCSFYKHTPGATPMTGFVEALLAELDR
                     QNDAHDLKPRTLYFGGGTPTALSETHLENLLSGLRSRLDFSGVAEFSVEANPRTVTPS
                     KADMMRRLGVTRVSLGIQAWDAETLHTLGRDHEPDEAGETWRVLQQAGFSSLNMDLMF
                     SIPGQPAEVWRRTVEKAVTLQPDHISAYNLNYEEDTEFFQRLRRGQYHEDDSRDAEHF
                     YLALDVLETAGYEHYEISNYARPGHRSLHNESYWLGRDYLGFGPGAFSTVADFRWKNV
                     ADTAEWMRRASSGNSAAEPAEEINDAARRVERFGLELRTARGLPLNLVLPAQRPMLET
                     LEKEGLLLVAGGRVRLTRAGKPLVDSVAVALMGN"
     gene            complement(6325..6741)
                     /gene="atpC"
                     /locus_tag="PLP58_12850"
     CDS             complement(6325..6741)
                     /gene="atpC"
                     /locus_tag="PLP58_12850"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009958353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F1 subunit epsilon"
                     /protein_id="HRJ09457.1"
                     /translation="MPLKLEIVTPEARIFSDEVDTVVLPGYEGEMGVLPAHAPLVTTL
                     LPGELRITKAGKTTEMAVGEGLVEVTGNTTRVLTDMAVHSDDIDEKAVEEALERARHS
                     LESLKHGEHQEEVAAVMAMIQRSTAQLHVKRKRRTV"
     gene            complement(6840..8297)
                     /gene="atpD"
                     /locus_tag="PLP58_12855"
     CDS             complement(6840..8297)
                     /gene="atpD"
                     /locus_tag="PLP58_12855"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018970036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HRJ09458.1"
                     /translation="MSNIGKIVQVIGPVVDVDFSASGKLPEIYNSLEITYDLGGKNSR
                     LVCEVQQHLGDGWVRSVAMSSTEGLKRGMSVTDTGAPITVPVGEEVLGRIFNVTGDAI
                     DDQPSPKTEKRYPIHRPAPALVDQDPSAQILETGIKVIDLICPFTKGGKVGAFGGAGV
                     GKTVVIMELINNIAKGHGGYSVFAGVGERTREGNDLYHEMIESDVIKVKKNGHDIERN
                     AQGGYICEPGSKVALVYGQMNEPPGARLRVALSALSMAEYFRDEKNQDVLLFVDNVFR
                     FSQAGSEVSALLGRTPSAVGYQPTLAAEMGAMQERITSTKSGSITSFQAVYVPADDLT
                     DPAPANTFAHLDSTVVLERSLAELGIYPAVDPLASVSKALAPEIVGEEHYRVARGVQR
                     VLQRYKDLQDIIAILGMDELSDDDKLTVFRARKLQRFLSQPFHVAEIFTGTPGQYVSV
                     KDTVKGFAEILDGKHDDVPEANFYMKGGIDTVPKS"
     gene            complement(8334..9215)
                     /gene="atpG"
                     /locus_tag="PLP58_12860"
     CDS             complement(8334..9215)
                     /gene="atpG"
                     /locus_tag="PLP58_12860"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009958351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F1 subunit gamma"
                     /protein_id="HRJ09459.1"
                     /translation="MPSTRDIRRRIKSVKNTAQITKAMQLVAAAKMKKAQDQAANGRA
                     YAELMNKILVALKESAEEGVHPYFSEGKGSKTLVLVMATDKGLCGALNTNLLKKLINT
                     RIEGEVEFVTIGRKASQGLSRLRKTLIADFPVKDPAKFAEVRVVGKFIQEKFLSGEYK
                     TVLVAFNNFINTVTQVPSIEQILPVNPVTLGGKRGFDETPGEINAAELPQYTFEPGAS
                     VVFETVLPQYVNGTLYQMLLEARASEHSSRMVAMKNATDNAKQMLKDLSLEYNKLRQA
                     AITNELLEITTAKMALE"
     gene            complement(9257..10786)
                     /gene="atpA"
                     /locus_tag="PLP58_12865"
     CDS             complement(9257..10786)
                     /gene="atpA"
                     /locus_tag="PLP58_12865"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009958350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HRJ09460.1"
                     /translation="MSNILQEIESQIAGLKTAVTKSNVGVVREIGDGAAKIEGLSDVM
                     LNEMIEFPGGLYGLALNLEEAEVGCVLLGSGEHIKAGDEVRTTGRLLSVPVGKGLLGR
                     VVNTLGEALDGKGEIKGDAQYPVEKIAPGIITRKSVSVPVQTGIMPIDAMIPIGRGQR
                     ELIIGDRSTGKTTIAVDTIISQAKQNKAAEQGKLQGHKPLYCIYVAIGQKQSNVARVV
                     KTLEDAGAMEYTVIVNASASDSAVNQYLAPYTGCAIGEWFMDQGMDALIVFDDLSKQA
                     VAYRQVSLVLKRPSGREAYPGDVFYLHSRLLERSARVNENYGGGSLTALPIIETQAGD
                     VSAYIPTNVISITDGQIFLETDLFYQGIRPAISVGLSVSRVGSAAQTKAIKKVSGTTK
                     LDLAQFRELAAFAQFGSDLDAATKAKLDRGARIVELFKQQQYQPKSLPVMVISLYAMQ
                     KGYFDDVAVDRIKECQGKLEEYLDTRKAPLLEKLANEKALDNVEADIKSALDDFKASW
                     K"
     gene            complement(10848..11243)
                     /locus_tag="PLP58_12870"
     CDS             complement(10848..11243)
                     /locus_tag="PLP58_12870"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007417686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HRJ09461.1"
                     /translation="MKISKEARRTSRQLFRACMVDGKLDESRVRLVVSQVAASKPRGY
                     IAILDSFQGMVKTELENQRALVESATPLTPDLQASLKTSLCQKYRRDLALEFQVRPEL
                     LGGIRVKVGSDVWDGSVKARLEALKASLS"
     gene            complement(11272..11775)
                     /locus_tag="PLP58_12875"
     CDS             complement(11272..11775)
                     /locus_tag="PLP58_12875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009958348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F0 subunit B"
                     /protein_id="HRJ09462.1"
                     /translation="MLEEISAQFGLSWGKFIAQALIFLIVYNILKKFAFGPILAMLEQ
                     RRQRIADGEAKLEKIAKDLAEAEKNAQAIIDKANADADRQIKEAGENARALAEKRQQD
                     AVHEAGQIIAKAREAAQLEHEQLMSQLKREFGRMVSEATSRVTGKVLNTDDQARINQE
                     TTAQVSL"
     gene            complement(11873..12115)
                     /locus_tag="PLP58_12880"
     CDS             complement(11873..12115)
                     /locus_tag="PLP58_12880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018290650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HRJ09463.1"
                     /translation="MITEIVSMGAEVASASGGVTGNIVGGLAGLGAALGVGMIGSKAA
                     EAVGRNPGAFGNILTISIIGMALAEGLGIIAFFAVK"
     gene            complement(12191..13072)
                     /gene="atpB"
                     /locus_tag="PLP58_12885"
     CDS             complement(12191..13072)
                     /gene="atpB"
                     /locus_tag="PLP58_12885"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000276 - mitochondrial
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_component: GO:0045264 - plasma membrane
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HRJ09464.1"
                     /translation="MLTTTPDSVSTLPLLASGVHSYASELFGAGFLSFFTNSVFTALV
                     VIGLLLWFSRKATSNMSLVPHKAQNFFELIVEFLFNQVEAVVGKKMAPKAFPLLATLF
                     IFILVTNWLGLFPGVGTIGWGPGTGPLSVKEVTEPLFRPATADLNMTLAMALIFFAFW
                     IYWTISEVGVWGFLVHTFGPKGGLRGLMGMVMIVIFFCVGLIELFSILLRNVTLPMRL
                     YGNIFAGESVLHTIGGILDGKGAVVSFLGSVFLPMPFYFMELLVGLLQAMVFTLLCAV
                     YISLSTAHDDSHGEEHH"
     gene            complement(13231..14019)
                     /locus_tag="PLP58_12890"
     CDS             complement(13231..14019)
                     /locus_tag="PLP58_12890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009958213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HRJ09465.1"
                     /translation="MQAAPTLFDLTGQRVWVIGGAGWLGQATVLTLAEMGASVLCADL
                     PGRSAAFLEKSSFAGDITTADLDAHDTDAIPRFVESHLASHGTPVGLVNLTYASTAKR
                     LADLTAAEFDQVNHGNLTSTFILTRAVAERMAAAGLGSIVLFSSMYGSVAPDERIYAG
                     AMNPNPVEYGVNKAGIRQMARYLAMHYGPHGVRCNSVSPGPFPNPSIQSAQPAFMRRL
                     VEKVPLGRVGVPVEIAGAVAFLLSDAASFITGIDLPVDGGWTAW"
     gene            complement(14025..>15336)
                     /locus_tag="PLP58_12895"
     CDS             complement(14025..>15336)
                     /locus_tag="PLP58_12895"
                     /inference="COORDINATES: protein motif:HMM:NF019223.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF1553 domain-containing protein"
                     /protein_id="HRJ09466.1"
                     /translation="ADGEETASYSTRTASMEGLQFATLGTWRFEADGQGFVLITNAGA
                     DGVVTVDAVQFLPADADMPAMPALTAASDMEKEVKARMTRVQREIKEIEKEGPTRAEA
                     MSVTEPDQPEDCRVHIRGNIRNLGAPVPRGFIQAALKGPVPAIPQDQSGRLQLAQWIT
                     SREQPLTARVFVNRVWQWIFGAGIVRSSDNFGVTGELPSHPELLDTLAIRFMEDGWNL
                     KRLVKDMVMSRAYRMDSQAATPAADPDNRLLSRMNRKRLDAECIRDAMLAASGTLDDR
                     WGGPNVADAKAVDSNDTGVQNLEYNYPFSDHRRSVYAAAFRNVRHPLFEVFDFADINQ
                     PIARRETGTIAPQALYLMNHPQVIELARSAADQVWKSQPPEHGLRLAWRRSLSRDPDN
                     DELRLAADYLDASISGNATGDEPRDAWARLIQTLWATPEFRFLR"
BASE COUNT         2740 a         4527 c         5138 g         2931 t
ORIGIN      
        1 cgcgctttcc agtgaacacc gcgctgaagg ctggagccta caccgtgcgc gcgcggctgg
       61 aggcgggcgg cgtgtccagc acgcgcgcgc tggattttca cattgtcccg cgccttcccg
      121 ccgtccgcat gccggtgatc atgtggggcg cgggcagcga tgaaataccg cgcctcaaag
      181 acatcggttt cacccacttc atcggtcttg gtgcgcagct cggcgagata tgggcgcaaa
      241 agaaggacgc gcctccagga gacgccgatt tcatcgcgcg caaccgcgcc gcgcttgacg
      301 ccgcgctggc agcggggctt ggcgtggtgg ccagtgtttc gcccgcccgc ttgttcgagg
      361 gcaagccgga attccaccgc gtggaccgcg agggcaggcc cttcccacgc gcgacgatct
      421 gcgcctcgat gccggagctg ccgccgtttt ttgagaacgt tgggcgctcc ctggcgcgcg
      481 cccatggcag ccatcccgcc ttcaccaccg tgctcgtcaa caccgaggtc cgagacggtt
      541 cgcgcccatc cttcaatgcc gtggaccgtg aaaactaccg cgccttcgcc ggcgcggaca
      601 tccctgcgga ggtggatcag cgcaccggtg tggactggac caggctcaag gatttcccgg
      661 tggatcgtgt cgtgcccgac gaccatccca tcctgaaata ctaccgctgg ttttggaccg
      721 tgggcgatgg ctggaacgcg ctccatagcg ccctgcacaa aggcgtgaaa tccgtcagca
      781 gccgacagtg gaccttcttc gatcccgccg tgcgccagcc ctccatcagc ggcgcgggcg
      841 gcacggtgga tgtgctctcg cactggacct acacctaccc ggatccgcag cgcatcggtc
      901 tttgcgcgga ccagctctta gccatgagca cggcgtcagg gcgcggccag aaggtcatga
      961 aaatgaccca gctcatctgg tatcgaagcc agaccgcgcc tgtgaagccg ggcaggccgg
     1021 agaatcccgt ggcctgggag gaccaagatc ccgacgccgc ttacatcacc atcgccccca
     1081 tgcacttgcg cgaggcattc tgggcaaaga tcgcccgccc tgtgcagggc atcatgtacc
     1141 acggctggca gtcgctggtg cccgtgacga acagcagcag cggctaccgc ttcaccaatc
     1201 ccaacacgat gcatgtgctc aaggaactca tccacgaggt cgtggagccg ctcggtcctg
     1261 cgctgatgaa gatccccgac gaaaggcacg aggtggcttt cctggagagc ttcacctccc
     1321 aagtcttcgc ccgccgcggc ggtcatggct acaatggcac ctggtctgcc gatgcatggc
     1381 tggcgctcca gcacgcgcat gtgcctgtgg acatcctgtt tgaggaaacc ctgctcaagg
     1441 acggcctcaa cgggcgcaga atcctcgtca tgaccgagtg cgacgtgctc agccaaagcg
     1501 tgctgagcaa aatccgcgag tggcaggcca aaggcggcaa aatcctcgcc gacgagcacc
     1561 tctgccccgc tttgaaagcc gactttgtca tcccttcctt caagcgctcc aaaaacgccg
     1621 ccgaggacaa ggcgcgcgtg cttgatctcg ccgcgcaaat cagcgggcaa accggcgttt
     1681 tcggtttgtc cccaggaccg caggctgaca cgccggaggt catcctgcgc gcgcgtcgcg
     1741 ccggcgacgc tcgctacctt ttcgccgtca acgaccggcg cgaggcgggc agctatgtcg
     1801 gccagcacgg cctggtgctg gaaaacggcc tgcccacgcg cgccatgctc gcctggccgc
     1861 aggatgcagt gcatgtgtat gatctcaccc gcgcgcgcca ggtcatcccg cagcgcgaag
     1921 atgaaggccg cctgcgatgg ccctgcgagc tcggcccctg tgacgggcgc ctttacatgc
     1981 tcacccccaa gccgctgctc agcctcaagc tcgacgcgcc cgactccgcc aagcccggcc
     2041 acagcgccac actcgccgtc acactcacca ccacccagga agcgcctttg aacgccgtcg
     2101 tcccggtcga ggtgcgtgtg cgcgatgcca atggcagacc cgccgagggc agcggccact
     2161 acgccaccga aggcggcagg ctcagcgtca cgctcgacat agcccccaac gaagaccccg
     2221 gctcgtggga aatacgcgtc cgcgaacttg cctccggcat ggagtccgcg agctggatgc
     2281 gcgtggaatg agcgccgccg cgcttcagtc cgccgcgcgc agcccgttga gcaaaagatt
     2341 gagggcctga atctgatcca tgtccttttt cttctccttc gccgcctgct ccgcgtcaaa
     2401 aagagtgtcg aagtcgtcaa agggcaccag gtccgcatgg atgcgggcac gccggcatga
     2461 gtttttaaaa gtcttgtcct cgaaccagcg caggatatcc gtcttttcgg attcgctggc
     2521 cgcaaaaccc gcgcccactg cggaggggat gcgcgtcagc gggcggaagc ctttcaacaa
     2581 gctgtaggcg caccaacggt catgctcgat gcgcgccagc gcttcttcag ccgccgccgc
     2641 cgtttgacct tttccgatca gtcgcttgcg cacgcgcgcc ttcacatgcg cgtgggccgc
     2701 cgcctggcgg ctggattcct tgtcggcctc ggaaatgccc gaccatgcgt ccacgcactt
     2761 gccgtcaaaa tcctcgatgg gcatgccaag gcttttccca aggccgaaag catagatgcc
     2821 gttcaagcgg cgcgccacgc ggtctatctc gtcaccgcgc accgccttct ttgagcagtc
     2881 gcccatgaaa tcggagaaga cgacgaccgg cagggtgggg tggtcgcggt tgagcgcctg
     2941 ctcgatgtca tcgcgataca catcctccgg cgtgttgtag tagagagtca gggtgatgtc
     3001 ccgtttgagc gccgcgcgct gccgctccag cacaggcgcg agcagtgccg ccagagccgc
     3061 ggactccggc gcgctttcca gggcgatgaa gaccgaggtc acctccttct tcgcgcgcag
     3121 ggcaagcccg ccctcaaaaa gatccaccag cgctcgctcg ctggaaggat gtttcaggaa
     3181 gagcaccacc ggaagcacct gcttctcaag ccagacccac tctggaaaag cgcagggctg
     3241 gccctccctc tccgcggctg agtagactgg atgacgcgcc accagcctgt ccctcgcggc
     3301 ctgcggatca ggtgaaagcc aggcgatgct caaggggcgg cctttctcga agtgtccgag
     3361 ttggagaaac tgttcgaaca gctcccaagc catcaatgac tctccgatga gcaccacacg
     3421 cgcgccagca gcatccgggg aggcggcgaa acggtccagc ggatgccgct ccagaagcat
     3481 ccgcgcggtc acggtctcgg cgttgaagat gcgcaagtcc agcccgtggt tttccggcag
     3541 cacatccttc aagatttgcg ctgtggccct gtccttgagg gagacaccgc agaccagcgc
     3601 ctggtcgtcg cttgtcttcc tccccaacgg agcgcctttg ccacgcccga aaaatatgcc
     3661 gccgccgccg cccagtttcg ccagctcatg cacgatgcgc atgttggtct cgtcatcctg
     3721 ggagcagaca aaagcatggc gcagcctggc gatgcccgcc tggcgaagga tggctggatc
     3781 accggcgtca ccttcgatca gcatggctcc ggcgcgtcgg cacaggtcgc gcgcggcctc
     3841 gtcgggctgc tgctcgatga ccagcgtcgg tctgcgccgg ctgccattct ccaccagcgc
     3901 ccggccgcgc cagcctagac cgcagatcag gtcatgcggc agaaaatgcg ccgtgaacca
     3961 gcgtctcagc caggcccaaa caccgaagac tttttcaaag aacgccgcca ggccaagccc
     4021 cgcgaagccc accgccagcc agcgcccaag ccgcacctgc catgccccgc cgccgcccag
     4081 ccccgtgtcc tccgtcagtc cctccatcac cagatgactc agcgccagga tggaatggtc
     4141 cagcatgctg attgtgtcac gttcgcaggc atccacgctc tcacgatagc cagccaggct
     4201 gaagaacacc gagaagaaaa acagcgccgc cagcagcagc gcccgcgtca ccggccagaa
     4261 gagtttgcgc cctgccaaac cggaaaagcg cgccagccgc gcaggctgga tggcgccgag
     4321 cgtgaacgcc tcggtggccg gatgcggatt cgactggcgc ggcaccgcga gccacaccac
     4381 gcgcagtgga gtccgtccct tctgcttgcg tatttgctgg cactcctggg acaccgcata
     4441 ggccagcgtg tcaccggtgc cgcccatgcc gccgccgtaa atgccatccc agagcgccac
     4501 aagcacatcc gcgttttccg ccagatgtct ccccgcctgc cggtagcact cctggcggtg
     4561 cttttcatcg gtctgtttgt ccgggcgttc cggcggcggc ggcggcggca gcggcacctc
     4621 atgcgccttc tgcgacagcg cggcaaagct ccattccgct ccgggagtga attttgcctc
     4681 atccgcctcc accacatccc gataccctgt tttgtcaaaa ggcaggaccc attccaatcg
     4741 cggtgtttta ccctccaccg cgccctccaa gaaaatcttt gccgccattt ggtccgcgcc
     4801 agccgatagc gaagtcaaaa caacgggatt gccagttatg cgtgcctgct cgtcttgcca
     4861 ccactttccc agcgctttct ggatggtcgg aaaatcacct gcattgaggt cacgatgtcc
     4921 aatcaaagcg tatctgcggc tcataaaggg atgtggaatt cctgccagca tgaggaaccg
     4981 acgcccgtgc cacaatccaa atcttggtca cgctgaaaaa atcatgcgtg ccagccgggg
     5041 cggcgtccaa attgcatccg agggtccgca atcttcccgg tttgaagttg ctcccccgcg
     5101 cttgaggcgc tggcattgct ccttcgcgcg cgaggcgcgc tgatcgcact cagtttccca
     5161 tcagtgccac agccacagag tccacaagcg gcttgcccgc gcgggtcagg cgcacgcgcc
     5221 caccggccac caagaggagg ccctcctttt ccagagtctc cagcatgggg cgctgagcgg
     5281 ggaggacaag gttcagcggc agtccccgcg cggtgcgcag ttccagaccg aagcgctcca
     5341 cgcgcctggc cgcgtcgttg atttcctcag caggctcggc agcgctgtta cccgatgatg
     5401 cgcggcgcat ccattcggct gtgtccgcca cgttcttcca gcggaagtct gccactgtgg
     5461 aaaaagcccc cggcccgaag ccgaggtagt cgcgccccag ccagtagctc tcgttgtgca
     5521 gggaacggtg accggggcgg gcgtagttgg aaatctcgta gtgctcgtag ccggcggtct
     5581 ccagcacgtc gagcgccaga taaaaatgct ccgcatccct ggagtcgtcc tcgtggtatt
     5641 gcccgcgccg cagccgctga aaaaactcgg tgtcctcctc gtagttcaga ttgtaggccg
     5701 agatgtggtc cggctggaga gtgacggctt tttccaccgt gcgacgccag acctccgcag
     5761 gctggccggg gatggaaaac atgaggtcca tgttcagact ggaaaatccg gcctgctgga
     5821 gcacacgcca ggtttctccg gcctcgtcag gctcatggtc gcgcccgagg gtgtgaagcg
     5881 tttcggcgtc ccaggcctgg atgccgaggc tgacgcgggt aactccgagc cgccgcatca
     5941 tgtccgcctt ggaaggggtg acggtgcgcg gattggcctc gacgctgaat tcagccacgc
     6001 cagagaaatc caaacgcgag cgcagaccgg agaggaggtt ttccaggtgc gtctcactga
     6061 gcgcggtggg cgtgccacca ccgaaataga gagtgcgcgg tttgaggtca tgcgcgtcgt
     6121 tttgacggtc caactccgcc agcaaagcct caacgaagcc tgtcatcggt gtcgctcccg
     6181 gtgtgtgctt gtaaaagctg cagtaggggc agacgcggtg gcagaagggg atgtggaagt
     6241 agaggtgctc gatcatccgc gccacaagga cggcagaatg cgcgggccgt cacgcggtag
     6301 tttaagcggg accgccttgc gggatcaaac cgtgcggcgc ttgcgcttga cgtggagctg
     6361 ggcggtgctg cgctggatca tggccatgac ggcagccact tcctcctggt gctcgccgtg
     6421 tttgaggctt tccagggagt gcctggcgcg ctccagggct tcctcgacag ccttttcgtc
     6481 aatgtcgtcg gaatggacgg ccatgtcggt tagcacgcgc gtggtgtttc cagtgacttc
     6541 caccaaacct tcgccaactg ccatctcggt ggtcttgccg gctttggtga tgcgcaattc
     6601 gcctggcagg agggtcgtca ccagcggggc gtgcgcgggg agcactccca tttcgccctc
     6661 gtagccgggt aggacgacgg tgtccacctc atcggaaaag atgcgggctt caggggtgac
     6721 gatttcgagt ttgaggggca tgtgggaagt tggcggttct ttgttcctgg ccgcaggttg
     6781 agggtgcttt cgtatgaggg cggaaccagc aactcacaac gatgaaccag gaacggtgat
     6841 caagacttgg gcacggtgtc aatgccgccc ttcatgtaga agttggcctc aggcacgtcg
     6901 tcgtgcttgc cgtcgaggat ttcggcgaaa cctttgacgg tgtctttgac ggagacgtac
     6961 tggccggggg tgccggtgaa gatttcggcc acatggaagg gctggctgag gaagcgttgg
     7021 agcttgcggg cgcggaagac ggtgagcttg tcgtcatcgg agagctcgtc catgccgaga
     7081 atggcaatga tgtcctgcag gtccttgtaa cgctgaagca cgcgctgcac gccgcgggcg
     7141 acgcggtagt gctcctcacc gacgatttcc ggggccagcg ccttcgagac ggaggcgagc
     7201 gggtccacag cggggtaaat gcccagctcg gcgagcgagc gctccagcac gacggtggag
     7261 tcaaggtggg cgaaggtgtt ggccggggcg gggtcggtaa ggtcgtccgc cggcacataa
     7321 acggcctgga aggaggtgat ggaaccgctt tttgtggagg tgatgcgctc ctgcatggcg
     7381 cccatttcgg cggccagggt gggctggtaa cccacggcgg agggcgtgcg gcccagaagg
     7441 gcggatacct cggaaccggc ctgggagaaa cggaagacgt tgtccacgaa gagaagcacg
     7501 tcctggttct tttcgtctcg gaagtactcg gccatcgaga gcgcggaaag ggcgacgcgc
     7561 agacgggcgc cgggaggctc gttcatctgg ccgtaaacaa gggccacttt ggagccgggt
     7621 tcgcagatgt aaccaccctg cgcgttgcgc tcaatgtcgt gcccgttctt tttcaccttg
     7681 atgacgtcgg actcgatcat ctcatggtaa aggtcgttgc cctcgcgcgt gcgctcgccc
     7741 acgccggcga agacggagta accgccgtgg cctttggcga tgttgttgat gagctccatg
     7801 atgacgacgg tcttgccgac accggcccca ccgaaggcgc cgaccttgcc gcccttggtg
     7861 aaggggcaga tgaggtcaat gaccttgatg ccggtctcaa gaatctgcgc ggagggatcc
     7921 tggtccacga gcgcgggagc tgggcggtgg atggggtagc gcttttcggt cttgggggag
     7981 ggctggtcgt caatggcgtc accggtgacg ttgaagattc ggcccaggac ttcctcaccg
     8041 acgggcacgg tgatgggtgc gccggtgtcg gtgacggaca tgccgcgttt caaaccctcg
     8101 gtggaggaca tggccacgga acgcacccag ccgtcgccga ggtgctgctg cacctcgcag
     8161 acgaggcggg agtttttgcc gccgagatca taggtgatct ccagcgagtt gtagatttcg
     8221 ggcagcttgc cgctggcgga gaaatccacg tccaccacgg gaccgatgac ttgaacgatt
     8281 ttgccgatgt tgctcataga ggtgattgga aaggtgaagt ttgaggcgcg ggattactcg
     8341 agagccatct tggcggtggt gatttcgagg agttcgttgg tgatggcggc ctggcgcagc
     8401 ttgttgtatt cgaggctgag gtccttgagc atctgcttgg cgttgtcggt ggcgtttttc
     8461 atggccacca tgcggctgct gtgctcggag gcgcgggctt ccagcagcat ctggtaaagc
     8521 gtgccgttga cgtattgcgg cagcacggtt tcgaaaacga cggaggctcc gggttcgaag
     8581 gtgtattgag ggagttcagc ggcgttgatc tcgccggggg tttcgtcaaa gccgcgcttg
     8641 ccaccgagag tcacgggatt caccggcagg atctgctcga tggagggcac ctgggtgacg
     8701 gtgttgatga agttgttaaa agccacgagc acagtcttgt actcgccgct aaggaacttc
     8761 tcctggatga atttgccgac gacacggact tcagcaaatt tggcggggtc cttgacaggg
     8821 aagtcggcga tgagcgtctt gcgcaggcgg gacaggccct gggaggcttt gcgcccgatg
     8881 gtcacaaact ccacctcgcc ctcaatcctg gtgttgatga gttttttgag aaggttggtg
     8941 ttcagcgcgc cgcacaagcc cttgtccgtg gccatgacga ggacgagggt tttggagcct
     9001 ttgccttcgg agaaataggg gtggactccc tcctcggcgc tctccttgag cgccacgagg
     9061 attttgttca tcaactcggc gtaggcgcgg ccgttggcgg cctgatcctg ggcttttttc
     9121 atcttcgcag ccgcgacgag ctgcatggcc ttggtgatct gggccgtgtt cttgaccgat
     9181 ttgattcggc ggcggatgtc gcgggtgcta ggcatgattt gaaatttgaa atttcagatt
     9241 tgaaatggcc gcgccctcac ttccaggagg ctttgaagtc gtcgagggcg gatttgatgt
     9301 cggcttccac gttgtcgagg gccttttcgt tggcgagttt ttcgagcagg ggggctttgc
     9361 gggtgtcgag gtattcctcg agcttgccct ggcattcctt gatgcggtcc acggccacgt
     9421 catcgaagta acctttctgc atggcgtaga ggctgatgac catgacgggc aggctcttgg
     9481 gctggtattg ctgctgcttg aagagctcca caatgcgcgc gccgcggtcg agcttggcct
     9541 tggtggcggc gtcgaggtcg gagccgaact gggcgaaggc ggcgagttcg cggaactggg
     9601 cgaggtccag cttggtggtg ccggagactt tcttgatggc tttggtctgc gcggcggaac
     9661 ccacgcggga gacggaaaga ccgacggaga tggcggggcg gatgccctga tagaagagat
     9721 cggtttcgag gaaaatctga ccgtcggtga tggagatcac gttggtcggg atgtaggcgg
     9781 acacgtcgcc ggcctgggtc tcgatgatcg gcagggcggt cagggagccg ccgccgtagt
     9841 tttcattcac gcgggcggaa cgctcaagaa ggcggctgtg gaggtagaac acgtcacccg
     9901 gataggcttc gcgaccggac gggcgcttga gcacgaggga aacctggcgg taagcgacgg
     9961 cttgcttgga aaggtcgtcg aagacgatga gcgcgtccat gccctggtcc atgaaccact
    10021 cgccgatggc gcaaccggtg tagggggcca ggtattggtt caccgcactg tcggaggcgg
    10081 aagcattgac gatgacggtg tactccatgg cgccggcgtc ttcgagggtc ttgacgacac
    10141 gggcgacgtt tgactgcttc tggccgatgg cgacgtagat gcagtagagg ggtttgtgcc
    10201 cctggagctt gccctgctcg gcggctttgt tttgcttggc ctgggaaatg atggtgtcca
    10261 cggcgatggt ggtcttgccg gtggagcggt ctccgatgat caattcgcgc tggccgcgtc
    10321 cgatggggat catggcgtca atgggcatga tgccggtctg aacgggaacg gagacggatt
    10381 tgcgggtgat gatgccgggt gcgatttttt cgaccgggta ttgcgcatca cctttgatct
    10441 cacccttgcc gtcaagagcc tctccaaggg tgttgacgac gcggcccagc aggcccttgc
    10501 cgacggggac ggagagcagg cggcccgtgg tgcggacttc gtcgccagcc ttgatgtgct
    10561 cgccggagcc gagaagcacg cagccgacct cggcttcctc aaggttgagg gccaggccgt
    10621 aaagaccgcc ggggaattcg atcatttcgt tgagcatgac atcgctcaag ccttcgatct
    10681 tggcggcgcc gtcaccgatc tcgcggacga cgccgacgtt ggatttggtg accgccgttt
    10741 tgaggccggc gatttgggat tcaatctcct ggaggatgtt gctcatgggt gctgcttgtt
    10801 tggggaaggg gtggggccgc taggacggcg aatggacagg gaaggattca ggacaggctg
    10861 gctttgaggg cttcgaggcg ggctttgacg gagccgtccc agacatcgga gccgactttg
    10921 acgcggatgc cgccgaggag ttcgggtctg acctggaact caagggcgag gtcgcgcctg
    10981 tatttttggc aaaggcttgt tttcaggctg gcctggaggt cgggtgtgag tggggtggcg
    11041 ctttcgacta gtgcgcgctg gttttccagt tcggtcttca ccatgccctg gaatgagtcg
    11101 agaatggcga tgtacccgcg gggcttggaa gcggcgactt ggctgaccac gaggcgcacg
    11161 cgggattcgt cgagctttcc gtcaaccatg caggcgcgga aaagctggcg ggaggtgcgg
    11221 cgtgcttcct tgctgatttt catggtcggt taatcggggg aagatggggg attacagcga
    11281 aacttgggcg gtggtttcct gattgatgcg ggcctggtcg tcggtgttga ggaccttgcc
    11341 ggtgacgcgg gacgtggcct cggagaccat gcgtccgaat tcacgcttga gctgggacat
    11401 gagctgctcg tgctcaagct gcgcggcctc gcgggctttg gcgatgatct ggcccgcctc
    11461 gtgaacggcg tcctgctggc gtttctcggc gagtgctcgg gcgttttctc cggcttcctt
    11521 gatctgacgg tccgcgtcgg cgtttgcctt gtctatgatg gcctgggcgt tcttctcagc
    11581 ctcggcgagg tctttggcga ttttttcaag cttggcctcc ccgtcggcga tgcgctgacg
    11641 gcgctgctcc agcatggcca gaatggggcc gaaggcgaac ttcttgagaa tgttgtaaac
    11701 gatgaggaag atcagcgcct gggcgatgaa cttgccccag gacaggccga attgggcgga
    11761 gatttcttcc agcatgagtt tgtgaggctg agtgagtggg tgactgattt ttgaaggggg
    11821 caccgggccg cgcgaggcgg cccggtgccg gaatcgtggc gttcgccgcg cgttatttga
    11881 cggcgaagaa ggcgatgata ccgagacctt cggcaagggc catgccgatg atgctgatgg
    11941 taaggatgtt gccgaaagcg ccggggttgc gaccgacggc ctcagcggcc ttcgagccga
    12001 tcatgcccac gccgagggcg gcgccgagac cggcgagacc gccgacgatg ttgccggtga
    12061 cgccgccgga ggcggaggcg acttccgcgc ccatggagac aatttcagtg atcatagtgt
    12121 gtgtgttctt ggtttgttgt ttgctcaccc ccgtccacgc tcgcggcatg gtcccggagg
    12181 caaattcgga tcagtggtgc tcctcgccgt ggctgtcgtc gtgcgcggtg gaaagactga
    12241 tgtaaaccgc gcagaggagg gtgaacacca tggcctggag caggccgacg agaagctcca
    12301 tgaaatagaa gggcatgggc aggaagacac tgccaaggaa actcacaacg gcgcctttcc
    12361 cgtctaggat gccgccgatg gtgtgcagca cgctttcacc ggcgaagatg ttgccgtaaa
    12421 gccgcatggg cagggtgaca ttccgcagaa ggatggagaa aagctcgatc aagccgacgc
    12481 agaagaagat gacgatcatg accatgccca tgaggcccct gaggccgccc ttcgggccga
    12541 aggtgtgaac gaggaaaccc cagactccca cttccgagat ggtccagtag atccagaacg
    12601 cgaagaagat gagcgccatg gcaagcgtca tgttcaggtc cgccgtcgcg ggacggaaca
    12661 gcggttcggt gacctctttg actgagagcg gcccggtgcc aggcccccag ccgatggtgc
    12721 cgacgccggg gaaaagcccc agccagtttg tgaccaggat gaagatgaag agggtggcta
    12781 gaagggggaa tgccttcgga gccatctttt tgccgacaac ggcctcgacc tggttgaaaa
    12841 gaaactcgac gatgagttcg aagaagttct gcgccttgtg cggcaccagg gacatgttgc
    12901 tggtggcctt gcgggagaac cagagcagca aaccgatgac gaccagggcg gtgaagaccg
    12961 agttggtgaa gaaactgaga aaaccggcgc cgaacaactc cgaagcgtaa ctgtggacgc
    13021 cgctggcgag gagcgggagc gtggagacgg aatcgggagt ggttgtcagc atgagtttca
    13081 cggacggagc cggacaggga aggtgattta gccggagcct tgggagaggc aagaggtatt
    13141 tgtgaaattt ttcacaaagt cggcgaagtc ttgattttac aaggcttcga gctccagtct
    13201 aaccgcaaac agcaacccgg cgcacggaga tcaccaggcc gtccagccgc cgtccacggg
    13261 caggtctatg ccagtgatga agctggcggc gtcactgaga agaaaggcca ccgcgccggc
    13321 gatctccacc ggcactccaa cacggccaag aggcactttt tccacgagcc tgcgcatgaa
    13381 agccggctgg gcgctctgaa tggaggggtt tggaaaaggg ccgggggaga cggagttaca
    13441 gcgcacgccg tgagggccgt agtgcatggc gaggtagcgc gccatctggc ggatgccggc
    13501 tttgttgaca ccgtattcga cagggttggg gttcatcgcg ccggcgtaga tgcgctcgtc
    13561 cggcgccacg ctgccataca tgctggagaa gagcacgatg ctgcccagtc cggcggcggc
    13621 catgcgctcg gcgacggcgc gggtgaggat gaaagtgctg gtgaggttgc cgtggttgac
    13681 ctggtcgaac tcggcggcgg tgaggtcggc gaggcgcttg gcggtggagg cgtaggtgag
    13741 gttgacgagg cccacgggcg tgccatggct ggcgaggtgg ctttcgacaa agcgcggaat
    13801 ggcgtcggtg tcgtgcgcgt cgaggtcggc ggtggtgatg tcgcccgcga aggaggattt
    13861 ttccaggaag gcggcgctgc gaccgggcag gtcggcgcag agcacgctgg cgcccatttc
    13921 cgcaagggtg agcaccgtgg cctgaccgag ccagccggca ccgccgatga cccagacgcg
    13981 ctgaccggtg aggtcgaaga gtgtgggggc ggcttgcatg gggattagcg gagaaaacgg
    14041 aactccggcg tggcccagag tgtctggatg aggcgcgccc aggcatcgcg cggctcgtcc
    14101 ccggtggcgt tgccggagat ggaggcatcc aggtagtcgg cggcgaggcg caactcgtcg
    14161 ttgtcgggat cacggctcag ggagcgccgc caggctaggc ggaggccatg ctccggtggc
    14221 tggcttttcc atacctggtc cgctgcggaa cgcgcgagtt cgatgacctg cggatggttc
    14281 atcaaataaa gagcctgggg agcaatggtg ccggtttcgc gtctggcgat gggctgattg
    14341 atgtcggcaa agtcgaagac ttcaaagagc gggtggcgca cgttgcggaa ggcggcggca
    14401 tagacgctgc ggcggtggtc gctgaaagga tagttgtact ccaggttttg cacgccggtg
    14461 tcattggagt ccacagcttt ggcgtccgcg acgttcgggc cgccccagcg gtcatcgagc
    14521 gtgccagagg cggcgagcat ggcgtcgcgg atgcattcgg cgtcgagtcg cttgcggttc
    14581 atgcgtgaga gcaggcggtt gtccgggtct gcggcgggag tggcggcctg gctgtccatg
    14641 cggtaagcgc ggctcatgac catgtctttg acgaggcgct tcaaattcca gccgtcctcc
    14701 atgaagcgga tggcgagcgt gtcgagcaac tccgggtggg agggaagctc gccggtgacg
    14761 ccgaaattgt cgctgctgcg cacaatgccg gctccgaaga tccactgcca gacgcggttg
    14821 acgaagacgc gcgcggtgag cggctgctcc cggctggtga tccattgggc gagctggaga
    14881 cgcccgcttt gatcctgcgg gatggcgggc acggggcctt tgagcgcggc ctggatgaag
    14941 ccgcgcggca cgggcgcgcc gaggttgcgg atgttgcccc ggatgtggac gcggcagtcc
    15001 tccggctggt ccggctcggt cacggacatg gcttccgcgc gagtggggcc ttccttttca
    15061 atctccttga tctccctttg cacccgggtc atgcgcgctt tgacctcctt ttccatgtcg
    15121 ctggcagccg tcagcgcggg catggccggc atgtccgcgt ccgccggcag gaactgcacc
    15181 gcgtccaccg tcaccacgcc gtcggctccc gcgttggtga tgagaacaaa gccctgtccg
    15241 tccgcctcga agcgccaggt gccgagggtg gcgaattgca gcccctccat gctcgccgtg
    15301 cgggtggaat agcttgccgt ctcctcgccg tcggcg
//
LOCUS       DAOMYJ010000117        15272 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Prosthecobacter sp. isolate AMR_MDS_5629
            NODE_1325_length_15272_cov_18.278241, whole genome shotgun
            sequence.
ACCESSION   DAOMYJ010000117 DAOMYJ010000000
VERSION     DAOMYJ010000117.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867850
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prosthecobacter sp. (wastewater metagenome)
  ORGANISM  Prosthecobacter sp.
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Verrucomicrobiaceae; Prosthecobacter.
REFERENCE   1  (bases 1 to 15272)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15272)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.30
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:34:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,742
            CDSs (total)                      :: 4,684
            Genes (coding)                    :: 4,676
            CDSs (with protein)               :: 4,676
            Genes (RNA)                       :: 58
            tRNAs                             :: 55
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15272
                     /organism="Prosthecobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1325_length_15272_cov_18.278241"
                     /isolate="AMR_MDS_5629"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1965333"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1228
                     /locus_tag="PLP58_12900"
     CDS             <1..1228
                     /locus_tag="PLP58_12900"
                     /EC_number="2.3.1.-"
                     /EC_number="3.6.1.57"
                     /inference="COORDINATES: protein motif:HMM:NF024698.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="bifunctional
                     UDP-2,4-diacetamido-2,4,6-trideoxy-beta-L-altropyranose
                     hydrolase/GNAT family N-acetyltransferase"
                     /protein_id="HRJ09467.1"
                     /translation="FDAAFGEALARAARRLLVVDDLGLPVPGAAALVLNQNITADAGL
                     YPPAAGGAGLLLGCRYALLRREFRAPPPVRDFAEPCRRLLVTLGGSDPQQVTGRVIAA
                     LRTLPGMEVRIIVGAVNPRLEELRALCAESGGRLEILAGVRDMVPLMDWAQLAISAGG
                     TSVLELASRALPTLLITVADNQAAICALLAEAGVMRHAGWHHELDVSTLAAMMEGLAE
                     DGPRRARMAELGRALVDGLGAVRVAGEMLARQAAEGIHLRRATLDDARLLLDWANDPV
                     TREVSFQSGVIAWETHLPWLERRLASAQSLLLIAEESAGVPLGMVRFDIGDTAAAGAT
                     ISINLAPAQRGRGLGPALILLGCRELRRAHGEMEIHAFIKPGNAASLRAFERAGFQPA
                     APADVAGQAALRMIQR"
     gene            1232..2287
                     /gene="pseI"
                     /locus_tag="PLP58_12905"
     CDS             1232..2287
                     /gene="pseI"
                     /locus_tag="PLP58_12905"
                     /EC_number="2.5.1.97"
                     /inference="COORDINATES: protein motif:HMM:TIGR03586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudaminic acid synthase"
                     /protein_id="HRJ09468.1"
                     /translation="MSDLCIHIGARAIGPGHPCYIIAEMSANHGQSLDQALRIVHAAK
                     EAGADAIKLQTYTADTLTLDCDHPCFRIGAGTLWEGRNLHQLYQEAFTPWDWQPRLKA
                     EAEKLGMDCFSTPFDATAVDFLEGMGVLCHKIASFEIVDLPLIRRVAATGKPVIMSTG
                     MATLAEIEEAVTTARAAGCRELALLKCNSGYPAPATEMNLRTIPHLSEAFGVPAGLSD
                     HTLDLAVPVAAVTLGACILEKHFTLDRSVPGPDNAFSLEPAEFAATVKAVRTAEAALG
                     RVHYAPSAREAPSRAFRKSLFVCADIAEGQPFTEENVRCIRPGDGLHSRHLDQVLASR
                     ARRALKRGTPLTLADLA"
     gene            complement(2448..5015)
                     /locus_tag="PLP58_12910"
     CDS             complement(2448..5015)
                     /locus_tag="PLP58_12910"
                     /inference="COORDINATES: protein motif:HMM:NF012311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HRJ09469.1"
                     /translation="MVRMDVSRIVVSALVMLMTWPYPVAADSKADLHAACEKGDVERV
                     RLLLNDADANAANEHGETPLMLAAKTGNFELCRALLWAGARATTSDNSGKKVRDHLKM
                     ESAGISPVNLLLRCYAYVQTHGIPARTRPSRPELVIISDNFIDHDNRDLKARYWVNQA
                     ELKGKAGVDDDQNGFVDDVHGWNASADEPLRVPLLANVADKAQEDLLRRFVTLLNRDD
                     DPGYGGDEWKDQTLEDLEGAYENPLVRQLGFDVLEESGVDLDDRVFTRMLAAASHGTH
                     VAGIVLKSSGGKALLHGMMHGRFQMPKEARSELVNLARRLAPRTISYEQFLLKMRQSL
                     LDNALKEGRRRSAYLRTTGAGVVNMSWGQTKTFFLRTAMQLQNIYREHGLDPDSIDSY
                     TCPAGLDLCGDLGFELLVNAAAELALLFHENPDVIFVVSAGNDAEDNDEELPSPAYLS
                     RFFPNVITVASVDEDNELSSFSNYGSASVQVAAPGEKILSTLIASQQGFMSGTSMASP
                     AVAGVAARIRAAHPRLTAADVRLILERSVNKLGNLESEVASGGVMDAEAALALAADWT
                     AGSRADLTLAALARPRKPVDEGILASEKDEGTNGHRSGEVSATAEDKGARVTAIGGFA
                     DQWRVVMKHGIGFLRQVVHPVGELPIGWIRDKWNDGWEITSIGGENEHWRVVMSHTGK
                     PGREQKLVGLDFDTGSLDDLQSIGWRITSHAGFVNSWVFVLTRDTGWKEQLYPLPGSF
                     DDSRRKWLHEHLAQGWRITSLAGDDNEKDRSQDSWSVVITRGTNLGEQVFTGPAAWPE
                     AWISANAKNGFQISNCSGFDDHWVVVMTKGSPSLPQTISEGGAWDDAWVQRLWKAEE"
     gene            5223..5996
                     /locus_tag="PLP58_12915"
     CDS             5223..5996
                     /locus_tag="PLP58_12915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009964672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HRJ09470.1"
                     /translation="MFSLSDKTVVITGAGSGIGRAVAVIFARQGAHVEVLDLSAGAAE
                     ETVAEITTAGGSARATACDVGEAETVRAVFAEITARRPRLDVLVNNAGIAHIGTLLTT
                     QEADMDRLYRINIKGVFNCAQAAVERMVAQGGGVVLNLCSIAAQMGLADRFAYSMTKG
                     AVLTMTYSIAKDFIHQGVRCNCICPARVHTPFVDGYLARTYPGREAEMFEKLSAAQPI
                     GRMARPDEVAALALYLCSDEAGFVTGCSYPLDGGAVNLR"
     gene            complement(6003..7694)
                     /locus_tag="PLP58_12920"
     CDS             complement(6003..7694)
                     /locus_tag="PLP58_12920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009963395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDSL-type esterase/lipase family protein"
                     /protein_id="HRJ09471.1"
                     /translation="MRRLLSLVFCLAAATLTAAPPEQRRILFLGDSITYAGTYIQIIE
                     AALIAQHPDKEYELLNLGLASETVSGLSEEGHAGGKFPRPNLHERLDRVLAAVKPQLV
                     IACYGMNCGIYHPLSEERARAYQQGMEKLRGKAAAIGASVLHLTPPVFDPAPIAARLL
                     PAGLDAYPQPYSGYNDVLSAYAQWLLDQRAQGWQVLDVHNAMKTALAAARGADPAFTF
                     AKDGVHPDAAGHLVMARPLLEHWGLKTTADGLPDHAFGADIFNLIKQKQALLRDAWLT
                     ETRHLRPGVKAGLPLSEAQAKARELDAKARELARTAHSAAFPGTVSEWQGFTRHDLTT
                     QAGLSAVVVAPRQAAPGRPWVWHGEFFGHKPAPDAALLARGFHIAYLKVPDLLGSPRA
                     VAHWNGFYAEMTGRYGLAPKAALVGLSRGGLYCYNWAAANPEKVSCIYADAPVCDFKS
                     WPGGKGKGKGDPKNWERVLKLWAFPDEAAALAARVNPVDNLAPLAKAGVPLLHVYGDA
                     DEVVPWEENTGVIAERYRALGGSITLIAKHGIGHHPHGLEDSAPIVEFIARHAAR"
     gene            complement(7740..9260)
                     /locus_tag="PLP58_12925"
     CDS             complement(7740..9260)
                     /locus_tag="PLP58_12925"
                     /inference="COORDINATES: protein motif:HMM:NF020039.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMP-30/gluconolactonase/LRE family protein"
                     /protein_id="HRJ09472.1"
                     /translation="MKPTTLLTTCLLTLAASAAPAAEWVSLFDGKTFTGWTFKGGKAE
                     YKIENGEITGVSVPKTPNSFLCTEKLYGDFILELEFKVDPNLNSGIQFRSNSLPDYKN
                     GQVHGYQCEIDPSDRGWTAGIYDEGRRGWLNNLANNDAARFAFKQGDWNHVRIEAIGD
                     RLRTFLNGVPAADLRDDMTKQGFIALQVHGVPDKFAGLQVRWRNIRLQENPAAGTPEA
                     PSAAALASVLPENPEVKKLAEGFTFTEGPALGPDGKIWFSDIPNERIHIYDPATGRTT
                     LHREQTGRANGLMLTPAGTLLACEGGARRITRQEGAGEVTVISDSFDGKPYNAPNDLD
                     IDGKGGIYFTDPGYGRKPEEMPLGVEGVYYATPDKDRTKPAKVVRVIDDLVRPNGVLV
                     SNDKKTLHVIDNGANSLWAYDIQGDGKVANGRKLCVFDPAEQRGGDGLTSDEHGNLYV
                     AANGIYVISPEGKLLGVIQVPERPANCVFGAKGTRTLYITAKTGFYSVQVNADGRR"
     gene            9347..10243
                     /locus_tag="PLP58_12930"
     CDS             9347..10243
                     /locus_tag="PLP58_12930"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRJ09473.1"
                     /translation="MAVLEVRNLTKRWRSDRLALDDVSFDLRQGEILGLLGHNGAGKS
                     TILGITLGMVRPDGGEVRIGGHSVQQERAQALQSIGAIYEAAHFYDYLTGWQNLRVLC
                     SLSGWWDEAEARQVLKLVNLGERAAHKTGTYSHGMRQRLALAQALLPRPKVLLLDEPT
                     DGLDPEGIREFREFVLRLRAEFGMTLLINSHLLAEVEQMCDRCVILKQGKKMYEGPVP
                     SQEKKTRQYALKTQDPVKAREALARAGASFDPVGGLVELPPHLTGPEMLRLIVESGTR
                     VESWQPHKPTLEEFYLGLTGAG"
     gene            complement(10227..11315)
                     /gene="hflC"
                     /locus_tag="PLP58_12935"
     CDS             complement(10227..11315)
                     /gene="hflC"
                     /locus_tag="PLP58_12935"
                     /inference="COORDINATES: protein motif:HMM:TIGR01932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0045861 - negative regulation of
                     proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protease modulator HflC"
                     /protein_id="HRJ09474.1"
                     /translation="MKPAASLLVLIAAVFLYILGMAALYTVGETEQIVITQFGKPVGE
                     PVKDAGLHVKLPFIQKVNRFEKRLLPWDGVSTAMPTRDKVYIEVNTFARWRISDPRLF
                     FEKLRDERGAISRLNDILGSETRNVIARHDFIEAVRTTKDRVVPREAAAGASIADARI
                     GVLPPIQKGRAVLEKEIFERSRDKVTIFGIELMDVRFKRINYNSSVSQTIYQRMISER
                     TQIAQRYKSEGAGEAAKILGQRERDLQTIESEAYRKVQEIEGAADAKATSIYAAAYDS
                     TSDARQLFHFLKTMETWKKSLTPDTSLILSTDSDLLRLLKSPDAPPSAPPATAANESG
                     DPLRKLPTLLQLASPASAETSGSLTPRP"
     gene            complement(11312..12310)
                     /gene="hflK"
                     /locus_tag="PLP58_12940"
     CDS             complement(11312..12310)
                     /gene="hflK"
                     /locus_tag="PLP58_12940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009959663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0045861 - negative regulation of
                     proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsH protease activity modulator HflK"
                     /protein_id="HRJ09475.1"
                     /translation="MPSPNTPQSPFSGPPNLQVNTTQILLGLAALLVIIGALSSFFQV
                     PAEAVGVVQRFGKYHDTAQPGLNFKLPFGIDRVTVLETRRQLKLEFGYGTGGATNEMQ
                     FNPDRQDQMLEKNMVTGDLNAAVVEWVVQFTISDARDYLFNFLAPQQTLRDMSESVMR
                     EVVGDRTVDEVLTIGRQDIETRAFERLAELTRTLKMGVQIDQVQLGNVNPPVEVKNSF
                     DEVNRAQQEKETAINQASSEYNRVIPEARGKAEQSISQAEGYATKRVNEAEGDAARFT
                     AVLAEYQKAPEVTRRRIYLETLGEVLPAIPGKVIVDETLPQFLPLMHLQQPEPVSP"
     gene            complement(12371..13561)
                     /locus_tag="PLP58_12945"
     CDS             complement(12371..13561)
                     /locus_tag="PLP58_12945"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HRJ09476.1"
                     /translation="MTVLWVNRREWRQPGPVINITVHNAWSFAAIGFESHLHLADGQP
                     SDTADDLREFYDLPPLPAFHVKRYRHTTLLPGIGGKAGVYSQTAAFARRLARRGRVAV
                     FTRDSSFLPWMRLLQADARIRCFYELHDLYADLTWRQEIDRVKHLRERWIERLLLPRL
                     GGLVCITRDMGAMYRHLFPRTPVISLPLGTKPAPPVDSEIKRLKRTVFYVGHMHGAKG
                     VSFLSGCAASLAAHGVRVEFWGGYEKDAAALTRSAAEKGLQDWIHAVPFQPPARLHEA
                     LAARASLGVVMLADTYYNRHLTCPVKALDYLSHGIPALGTALPSVREVLGDAGIWIKA
                     DDQQGFVSEAMRLLDCPATYSEAVNRARERAEEITWQNRARALADFARNRFEPAHGWQ
                     DRER"
     gene            complement(13568..14698)
                     /gene="holA"
                     /locus_tag="PLP58_12950"
     CDS             complement(13568..14698)
                     /gene="holA"
                     /locus_tag="PLP58_12950"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR01128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HRJ09477.1"
                     /translation="MPAPKKSASAASQIHVVLGTDDARVKEAAMQIVRRLTPPDAGEF
                     ANEIIEGNADNAEHAGRICGQVIQALQTLPFFGGAKVVWLRAANFLGESQTGKAQDSV
                     EGFESILDVIEAGLGPEVRFVLSASSIDKRRTAWKRLGKLANIEIFDKPDTSKQGWEG
                     PVLAHAQKIARSLGLVFESGALELLVQMAGDDTRQLENEIEKLDLYLGERRRCGIRTV
                     RGLVSQSRAGVLWDLGNAIGKRDLPRALSLLDVLLYQGQNAVGVLLAAIVPRVRSMLM
                     ARELASKHRVNRGNYNAFCTSLEALPPAVTAHIPRKKDGTGFNAYPIFLALAEAASFT
                     LDELEGALCSCLDANLKLVTTQLDNKVVLERLLVGMLSPARA"
     gene            complement(14834..>15272)
                     /locus_tag="PLP58_12955"
     CDS             complement(14834..>15272)
                     /locus_tag="PLP58_12955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ09478.1"
                     /translation="LVSNNNAPASGAPRQPRKTGVISAMARSQSWDSPAFEPLSFLMT
                     DSPAAPPPRPAARRASLASIIHGGDAGAENDSALAPDDLTRIRGITPGIQQKLRSLGV
                     RRYEHIASWTPQQVRDISRHLAFKDRIEREDWTGQARDLAAVR"
BASE COUNT         2373 a         4468 c         5617 g         2814 t
ORIGIN      
        1 ctttgacgcg gctttcggcg aggcgctggc gcgggcggcg cggcggctgc tggtggtgga
       61 tgatctgggg ctgccggtgc cgggggcggc ggctctcgtt ttgaatcaaa acatcacggc
      121 ggatgcgggg ctgtatccgc cggcggctgg cggtgccggg ctgctgctgg gctgccggta
      181 tgcgctgctg aggcgggagt tccgcgcgcc gccgccggtg cgggactttg cggagccgtg
      241 ccggcgcctg ctggtgaccc tgggcggcag tgatcctcag caggtcacgg gcagggtcat
      301 cgccgcgctg cggacgctgc cggggatgga ggtgcgcatc attgtcggcg cggtgaatcc
      361 gcgtctggag gagctgcggg ctctgtgcgc ggagagcggc gggcgtttgg aaatcctggc
      421 cggggtgagg gacatggtgc cgctgatgga ctgggcgcag ctcgccatca gcgcgggcgg
      481 gacgagcgtg ctggagctgg cctcgcgcgc tttgccgacg ctgctgatca cggtggcgga
      541 caaccaggcg gcgatctgcg cgctattggc ggaggcgggg gtgatgcgcc atgccggctg
      601 gcatcatgag ctcgatgtgt caacgctggc tgccatgatg gaggggctgg ctgaggacgg
      661 gccgcgcaga gccaggatgg cggagctcgg gcgcgcactc gtggacggcc tgggcgcggt
      721 gcgggtggcg ggggagatgc tcgcgcgcca ggcggcggag ggcattcact tgcggcgcgc
      781 gactctggat gacgcgcggc ttttgctcga ctgggcaaat gaccctgtga cgcgggaggt
      841 gtccttccaa tccggggtca tcgcctggga gacgcatctg ccctggctgg agcgccgcct
      901 ggcctcggcg caaagtctgc tgctgatcgc cgaggaaagc gcgggcgtgc cgctgggcat
      961 ggtgcgcttt gacatcgggg acacggcggc agcaggcgcc acgatctcga tcaacctggc
     1021 gccggcgcag cgggggcgcg gcctgggccc ggcgctgatt ttgctcggct gccgggagct
     1081 gcgccgggcg catggggaga tggaaatcca cgccttcatc aagcccggca atgcggcctc
     1141 cctgcgcgcg ttcgagcggg cgggtttcca gccggccgcg cctgcggatg tggctggaca
     1201 agccgcgctg cgcatgattc agaggtgagg catgagcgat ctctgcatcc acatcggcgc
     1261 gcgcgccatc ggccccgggc acccctgcta catcatcgcc gagatgtcgg ccaaccacgg
     1321 ccagtcgctg gaccaggcgc tgcgcatcgt ccacgcggcg aaggaggcgg gggcggatgc
     1381 catcaagctc cagacctaca cggcggacac gctgacgctg gactgtgacc atccctgctt
     1441 ccgcatcggc gcgggcacgc tgtgggaggg gaggaatctg caccagcttt atcaagaggc
     1501 cttcacgccc tgggactggc agccgcgcct gaaggccgag gcggaaaagc tgggcatgga
     1561 ctgcttttcc acgcccttcg acgccactgc ggtggatttc ctggagggca tgggcgtgct
     1621 ctgccacaag atcgcgtcct tcgagatcgt ggacctgccc ttgatccgcc gtgtggcggc
     1681 cacggggaag ccggtcatca tgtccaccgg catggccacg ctggcggaga tcgaggaggc
     1741 ggtgaccacc gcgcgcgcgg cgggctgccg ggagctggcg cttttgaaat gcaacagtgg
     1801 ttatcccgcg ccggccacgg agatgaatct gcgcacgatc ccgcatctgt cggaggcctt
     1861 tggcgtgccg gcggggctct ccgaccacac gctggacctg gccgtgccgg tggctgccgt
     1921 gacgctgggg gcctgcattt tggaaaagca cttcacgctg gaccgcagcg tgccggggcc
     1981 tgacaacgcc ttttccctgg agcctgcgga gttcgccgcc acggtgaagg ccgtgcgcac
     2041 cgcggaggcc gcgctgggcc gcgtccatta cgcgccctcc gcgcgggagg cgccgagccg
     2101 cgccttccgc aagagcctct tcgtgtgcgc cgacatcgcc gaagggcagc ccttcacgga
     2161 ggaaaatgta cgctgcatcc gccccgggga cggcctgcat tcgcggcatc tcgatcaagt
     2221 gctggcctcc cgcgcgcgcc gtgctctgaa gcggggcacg ccgctgacgc tcgcggactt
     2281 ggcgtgaaag tggcgtcggg tggctgcggt cattaaagat ggcggcagtg gctggaactg
     2341 gggcgctccc atgcatgatc ctgggcgatt ggaaattctt ccgccccgga agtggctgaa
     2401 tgatcgtggc aagaagggtt tgctgcggag acgggacggt cttgacctca ttcctccgcc
     2461 ttccaaagac gttgaaccca cgcgtcatcc catgcgccgc cttcgctgat cgtttgcggc
     2521 aaggaggggc tgcccttggt catcaccaca acccagtggt cgtcgaagcc tgagcagttg
     2581 gagatttgaa atccattctt cgcattcgcg gagatccacg cttcgggcca ggcggcgggg
     2641 ccggtgaaga cttgctcgcc aaggttggtg cctcgcgtga tgacgactga ccaactgtcc
     2701 tgggagcggt ccttttcatt gtcgtcgccg gcgagagagg tgatgcgcca gccctgggcg
     2761 agatgctcgt ggagccactt tcgccgcgag tcgtcgaatg acccgggcag cgggtagagt
     2821 tgttctttcc aacccgtgtc ccgcgtcagg acgaaaaccc acgaattgac aaagcccgcg
     2881 tgactggtga tgcgccagcc gatgctctgg agatcgtcaa gggagcctgt gtcaaaatcg
     2941 agtccgacga gtttttgctc gcggcctggc ttgccagtgt gggacatgac gacgcgccag
     3001 tgctcgttct caccgccgat gctggtgatc tcccagccgt cattccactt gtccctgatc
     3061 cagccgatgg gcaattcccc caccggatga acgacctgcc gcaggaaacc tatgccgtgt
     3121 ttcatgacca cgcgccattg atccgcgaag ccgccgatgg cggtgacgcg cgcgcctttg
     3181 tcctccgcag tggcggagac ttcgccggag cggtgcccgt tcgttccctc atctttctcc
     3241 gaggctaaaa tgccctcatc cactggtttc ctgggcctgg cgagcgcggc gagagtaagg
     3301 tccgcccttg atccggctgt ccaatccgct gcgagtgcca aggctgcctc cgcgtccatc
     3361 accccgccgg aagccacttc ggactcaagg ttccccagct tgttgacgga gcgctccaga
     3421 atgagccgca catccgccgc cgtgagccgc ggatgtgcgg cgcggatgcg cgcggccacg
     3481 ccggcaacgg cgggcgaggc catggacgtg ccggacatga agccctgctg gctggcgatg
     3541 agggtggaga gtatcttctc acctggcgct gccacctgca cgctggcgga gccgtagttg
     3601 gagaaagagg aaagctcgtt gtcctcgtcc acggatgcca cggtgatgac attcgggaaa
     3661 aagcgcgaga ggtaggcggg cgacggcagt tcctcgtcat tgtcctccgc atcattcccg
     3721 gctgagacaa cgaagatgac atccggattc tcatggaaga gcagcgccag ctcggcggcc
     3781 gcattcacca gcagctcgaa accaagatcg ccgcacagat cgaggccggc cgggcaagtg
     3841 tagctgtcaa tcgagtccgg gtccaggcca tgctcccggt agatgttctg gagctgcatc
     3901 gcggtgcgca ggaaaaaggt ctttgtctgc ccccagctca tgttcaccac gccggctccc
     3961 gtggtcctta aataggcgct gcggcggcgt ccctccttga gcgcgttatc caaaaggctc
     4021 tggcgcattt tcagtagaaa ctgctcatag ctgatcgtgc gcggcgcgag ccgccgcgcc
     4081 agattgacga gttccgaacg ggcctccttc ggcatctgga agcgcccgtg catcatgccg
     4141 tgcagcagcg cctttccacc gctgcttttc agcacgatgc cggcgacatg cgtgccgtgc
     4201 gaggcggccg caagcatgcg cgtgaagacc ctgtcgtcga ggtccacgcc gctttcctcg
     4261 agcacgtcaa agccgagctg gcgcacgagc ggattctcat aggcgccctc aaggtcctcc
     4321 aatgtctgat ctttccattc atcgcctcca tagccgggat catcatcgcg gttgaggagt
     4381 gtcacaaaac ggcgcaggag gtcctcctgc gccttgtccg ccacattcgc cagaagcgga
     4441 acgcgcaggg gttcgtcggc ggaggcgttc cagccatgca catcatccac gaaaccgttt
     4501 tgatcatcat ccacgccggc ttttcccttc aactcggctt ggttgaccca gtagcgcgct
     4561 ttcaggtcgc ggttgtcgtg gtctatgaag ttgtcgctga tgatgacgag ctccggtctg
     4621 gaaggcctcg tccgcgcggg tattccatga gtctgcacat aagcgtagca gcgcagcagc
     4681 aggtttaccg gggagatgcc cgctgattcc atcttcaaat gatcgcgcac ctttttgccg
     4741 gaattgtcgg acgtcgtcgc ccgcgccccg gcccatagga gggcgcggca caactcgaag
     4801 ttgcccgttt tcgcggcgag catgagcggc gtctcgccat gttcgttggc ggcgttcgca
     4861 tccgcgtcat tcagcagaag ccgcacgcgc tccacatctc ccttttcaca ggcggcatgc
     4921 agatcggcct tggaatccgc cgcgaccgga taaggccagg tcatcagcat gacaagcgcg
     4981 gagacaacaa tacgggagac atccatgcgg accattccag catgaatccc actctagtgc
     5041 cagcttgatc tgtccggcac cgccggactc ggcgccggcc tccccggcgc ggcggcgcgg
     5101 gcatagcacc gatgctggct ggctgcggtc attgaagatg ccggcagatt cagggaggcc
     5161 cgcggaggag tggtcaaaag tttcccttgg cagggggcgc tggctttcca tgatcggcgg
     5221 acatgttttc cctttcagac aagacggtgg tgatcacggg cgcgggttcg ggcatcggca
     5281 gggcggtggc ggtgatcttc gcccgccagg gcgcgcatgt ggaggtgctg gatctctcgg
     5341 cgggggcggc ggaggagacg gtggcggaaa tcacgacggc gggcggcagc gcgcgcgcca
     5401 cggcctgtga cgtgggggag gcggaaacgg tgcgggcggt ttttgctgaa atcacggcca
     5461 ggcggccaag gctggacgtg ctggtgaaca atgcgggcat cgcgcacatc ggcacgctgc
     5521 tgacgacgca ggaggcggac atggaccggc tctaccgcat caacatcaag ggtgtcttca
     5581 actgcgcgca ggcggcggtg gagcggatgg tggcgcaggg cgggggggtg gtgctgaacc
     5641 tgtgctccat cgcggcgcag atggggctgg cggaccgctt cgcctattcg atgacgaagg
     5701 gcgcggtgct gacgatgact tactcgatcg ccaaggactt catccaccag ggagtgcgct
     5761 gcaactgcat ctgcccggcg cgagtccaca cgccgtttgt ggacggctac ctggcgcgca
     5821 cttatccggg ccgggaggcg gagatgtttg agaagctcag cgcggcgcag cccatcgggc
     5881 gcatggcgcg gcctgacgag gtggcggcgc tggcgctcta cctgtgcagt gacgaggcgg
     5941 gcttcgtcac cggctgctcg tatccgctgg acggcggggc ggtgaatttg cgctgagccg
     6001 cctcaacgcg cggcgtggcg ggcgatgaat tcgacgatgg gggcgctgtc ctccaggccg
     6061 tgcggatggt ggccgatgcc gtgcttggcg atgagggtga tgctgccgcc gagggcgcgg
     6121 tagcgctcgg cgatgacgcc ggtgttttcc tcccagggca cgacctcgtc ggcgtcgcca
     6181 tacacatgca ggagggggac gccggctttg gccaggggcg cgaggttgtc cacggggttg
     6241 accctggcgg cgagggcggc ggcctcgtcg gggaaggccc agagcttgag cacgcgctcc
     6301 cagtttttgg gatcgccttt tcctttgccc ttgccgccgg gccagctttt gaaatcacag
     6361 accggagcgt ccgcgtagat gcaggagact ttttccgggt tcgcggcggc ccagttgtag
     6421 caatacaagc cgccgcggct gagaccgacg agggcggctt tcggggcgag tccgtatctg
     6481 cccgtcattt cggcataaaa gccattccag tgcgccacgg cccggggtga gccgagcagg
     6541 tcgggcactt tcaggtaggc gatgtggaag cctctggcga gcagtgccgc atccggcgcg
     6601 ggcttgtggc cgaagaactc gccgtgccag acccaggggc ggcccggcgc ggcttgcctg
     6661 ggtgcgacga ccacggcgga aagtccggcc tgggtggtga gatcatgccg tgtgaagccc
     6721 tgccactcgg agacggtgcc ggggaaggcg gcggagtgtg ccgtccgcgc gagttcgcgg
     6781 gccttggcgt cgagttcgcg ggctttggcc tgcgcctcgg agagcggcag gccggctttg
     6841 acgcctgggc gcaggtggcg ggtctcggtg agccaggcgt cccggagcag agcctgcttt
     6901 tgcttgatga ggttaaagat gtccgcgccg aaggcatggt cgggcaaacc gtcggcggtg
     6961 gttttcagcc cccaatgctc caaaagcggg cgcgccatca cgaggtggcc ggcggcatcg
     7021 ggatgcacgc cgtctttggc gaaggtgaag gcggggtcgg cgccgcgggc tgcggccagg
     7081 gcggtcttca tggcgttgtg gacatcgagc acctgccagc cttgcgcgcg ctggtcgagc
     7141 agccactggg cgtaggcgga gagcacgtca ttgtagccgc tgtagggctg cggataggcg
     7201 tcgagaccgg cgggcagcag gcgcgcggcg atgggcgcgg ggtcgaatac cggcggggtc
     7261 aaatgcagga cacttgcgcc gatggcggcg gcttttccgc gcagcttctc catgccctgc
     7321 tggtaggcgc gggcgcgctc ctcgctcaga gggtggtaga tgccgcagtt catgccatag
     7381 caggcgatga cgagctgcgg cttcacggcg gcgagcacgc ggtccaggcg ctcgtgcaga
     7441 ttcggacgcg gaaacttgcc gccggcgtgg ccctcctcgg agaggccgga gaccgtctcg
     7501 ctggcgaggc cgagattcag cagctcgtat tccttgtccg ggtgctgcgc gatgagcgcc
     7561 gcctcgatga tctgaatgta ggtgcctgcg taggtgatgc tgtcgccaag aaaaaggatg
     7621 cgcctctgct ccggcggggc ggcggtgaga gtggcggcgg cgaggcagaa gacgaggctg
     7681 agaaggcggc gcatgggttg tggaatacgc gccgctttcc ggcgagtcat caggatttgt
     7741 cagcgccggc cgtcggcgtt gacttggacg ctgtagaagc cggtcttcgc cgtgatgtaa
     7801 agcgtgcgcg tgcctttggc gccgaagacg cagttggcgg ggcgctcggg cacctggatg
     7861 acgccgagca gcttgccttc ggggctgatg acgtagatgc cgttggcggc cacgtagagg
     7921 ttgccgtgct cgtcgctggt gagaccgtcg ccgccgcgct gctcggcggg gtcgaagacg
     7981 cagagtttgc ggccgttggc cactttgccg tcgccctgga tgtcgtaggc ccagaggctg
     8041 ttggcgccgt tgtctatgac gtggagggtc tttttgtcgt tggagacgag cacgccgttg
     8101 gggcgcacga ggtcgtcaat gacgcggacg actttggcgg gcttggtgcg gtctttgtcg
     8161 ggcgtggcgt aatagacgcc ttcgacgccg aggggcatct cctcgggttt gcggccatag
     8221 ccggggtcgg tgaagtagat gccgcctttg ccatctatgt cgaggtcgtt gggggcgttg
     8281 tagggcttgc cgtcgaagct gtcgctgatg acggtgacct cccccgcgcc ctcctggcgg
     8341 gtgatgcggc gggcgccgcc ttcgcaggcg agcagggtgc cggcgggggt gagcatgagg
     8401 ccgttggcgc ggccggtctg ctcgcggtgc agggtggtcc ggccggtggc ggggtcataa
     8461 atgtggatgc gctcgttggg gatgtcgctg aaccagattt tgccgtccgg gccgagggcg
     8521 gggccttcgg tgaaggtgaa gccttcggcg agttttttca cttccgggtt ctccggcagc
     8581 acgctggcga gggcggcggc ggagggcgct tctggagtcc cggcagcggg gttttcctgg
     8641 aggcggatgt tccgccagcg cacttgcagg ccggcgaatt tgtccggcac gccgtggact
     8701 tggagggcga tgaagccctg cttcgtcatg tcatcccgca ggtcggcggc gggcacgccg
     8761 ttgaggaagg tgcgcaggcg gtcgccgatg gcctcgatgc ggacgtggtt ccagtccccc
     8821 tgcttgaagg caaagcgggc ggcgtcgttg ttggcgaggt tgttgagcca gccgcggcgg
     8881 ccttcgtcat aaatgccggc ggtccagccg cggtcgctgg ggtcaatctc gcactggtag
     8941 ccgtggactt ggccgttttt gtaatcgggc aggctgttgc tgcggaactg gatgccggag
     9001 ttcaggttgg ggtccacttt gaactccagc tccaggatga agtcgccata gagcttctcg
     9061 gtgcagagga agctgttcgg cgtcttcggc acgctgacgc cggtgatctc gccgttttcg
     9121 attttatact cggccttgcc gcctttgaag gtccagccgg tgaaggtttt gccgtcaaaa
     9181 agggagaccc actcggcggc gggcgcggcg ctggcggcga gggtgaggag gcaggtggtg
     9241 agaagcgtgg tcggtttcat ggcggggctg agtgtgcgcg tctggcgcgg gggcgcaatc
     9301 ttttccgctt tgcgcctttg ccgcgtccgt ctatagacag cgccgcatgg ccgtcctcga
     9361 agtccgcaac ctcaccaaac gctggcgcag cgaccggctg gcgctggatg atgtcagctt
     9421 tgatttgcgc cagggggaga tcctcggcct gctcggccac aacggcgcgg gcaagagcac
     9481 catcctcggc atcaccctcg gcatggtgcg tccggacggc ggggaggtgc gcatcggcgg
     9541 ccactccgtg cagcaggagc gcgcgcaggc gctgcaaagc atcggcgcca tctatgaggc
     9601 cgcgcatttt tacgactacc tcaccggctg gcagaacctg cgcgtgctgt gctccctctc
     9661 cggctggtgg gacgaggccg aggcgcgcca ggtgctgaaa ctggtcaatc tcggcgagcg
     9721 cgccgcgcac aagacaggca cctacagcca cggcatgagg cagcggctgg ccctggccca
     9781 ggcgctgctg ccgcgcccga aggtgctgtt gctggacgag ccgacggacg gtctcgaccc
     9841 ggagggcatc cgcgagttcc gcgagttcgt gctgcggctg cgcgccgagt tcggcatgac
     9901 cctgctcatc aactcccacc tgctggccga ggtggagcag atgtgcgacc gctgcgtgat
     9961 cctcaagcag ggcaaaaaga tgtacgaggg gcctgtgccc tcgcaggaaa aaaagacgcg
    10021 gcagtatgcg ctcaagaccc aggatcccgt gaaagcgcgg gaggcgctgg cccgcgcggg
    10081 ggcgtccttt gacccggtgg gcggcctggt ggagctgccg ccgcacctca cagggccgga
    10141 gatgctgcgg ctcatcgtgg agagcggcac gcgcgtcgag tcctggcagc cgcacaagcc
    10201 gacactggag gagttttatc tgggcctcac gggcgcgggg tgaggctgcc ggaggtttca
    10261 gcgctggcgg gactggcgag ctggagcagg gtggggagct ttcgcagggg atcgccgctt
    10321 tcgttcgcgg cggtggccgg cggcgcggag ggcggcgcgt cgggggactt gagcaggcgc
    10381 aggaggtcgc tgtcggtgct gaggatgagg ctggtgtcgg gagtgaggga ctttttccag
    10441 gtctccatgg ttttgaggaa gtggaaaagc tgcctggcat ccgaggtgga atcataagcg
    10501 gcggcgtaga tgctggtggc tttggcgtcg gcggcaccct cgatctcctg gactttgcgg
    10561 taggcctcgg actcgatggt ctgaaggtcg cgctcgcgct ggccgaggat tttggcggcc
    10621 tcgccggcgc cctcggattt gtagcgctgg gcgatttggg tgcgctcgct gatcatgcgc
    10681 tggtaaatgg tctgggagac ggaggagttg tagttgatgc gcttgaagcg cacgtccatg
    10741 agctcgatgc caaagatggt gaccttgtcg cgggagcgtt cgaagatttc tttttcgagg
    10801 acggcgcggc ctttctgaat gggaggcagg acaccgatgc gcgcgtcggc gatggaggcg
    10861 cctgcggcgg cctcgcgcgg gacgacacgg tccttggtgg tgcggacggc ctcaatgaag
    10921 tcgtggcgcg caatgacgtt gcgggtctcg ctgccgagga tgtcgttgag gcgggagatg
    10981 gcgccgcgct cgtcgcggag cttttcaaag aagaggcggg gatcgctgat gcgccagcgg
    11041 gcgaaggtgt tgacttcgat gtacaccttg tcgcgcgtgg gcatggcggt ggagacgccg
    11101 tcccagggca ggaggcgctt ttcaaagcgg ttgacctttt ggatgaaggg cagcttcaca
    11161 tgcaggccgg catccttgac gggttcgccg acgggtttgc cgaactgggt gatgacgatc
    11221 tgctcggtct cgcccacggt gtagagggcg gccatgccga ggatgtaaag aaagacggcg
    11281 gcgatgagga cgaggaggga ggcggcgggc ttcatgggga cacgggttcg ggctgctgca
    11341 gatgcatgag gggaagaaac tggggaaggg tttcatccac gatgaccttg ccgggaatgg
    11401 cggggaggac ctcgccgaga gtttcgaggt aaatgcggcg gcgtgtgact tcgggggctt
    11461 tttgatactc ggcgagcacg gcggtgaagc gcgcggcgtc gccttcggcc tcgttgacgc
    11521 gcttggtggc gtaaccctcg gcctggctga tgctctgctc ggccttgccg cgcgcctcgg
    11581 ggatgacgcg gttgtactcg ctgctggcct ggttgatggc ggtttccttt tcctgctggg
    11641 cgcggttgac ctcgtcgaag ctgtttttga cctcgacggg cggattgacg ttgccgagct
    11701 ggacctggtc aatctggacg cccattttaa gagtgcgggt gagctccgcc agccgctcga
    11761 aggcgcgggt ctcgatgtcc tggcggccga tggtgaggac ctcgtccacg gtgcggtcgc
    11821 cgacgacctc gcgcatgacg gactccgaca tgtcccgcag ggtctgctgg ggggcgagga
    11881 agttgaagag gtagtcgcgc gcgtcggaga tggtgaactg gacgacccat tcgacgacgg
    11941 cggcgttgag gtcgccagtg accatgtttt tctccagcat ctggtcctgg cggtcgggat
    12001 tgaactgcat ctcgttggtg gcgccgccgg tgccgtaacc gaattcgagt ttcaactggc
    12061 gccttgtttc caggacggtg acgcggtcaa tgccgaaggg cagtttgaag ttcaagccgg
    12121 gctgcgcggt gtcatgatac ttgccgaagc gctggacgac gccgacggcc tcggcgggca
    12181 cctggaaaaa gctggagagc gcgccgatga tgacgaggag agcggccagg ccgagcagga
    12241 tctgggtggt gttgacctgc aaattcggcg gcccggaaaa gggggactgc ggcgtgttgg
    12301 gtgagggcat ggggcaaggc tagctgtgat tgagtgtgcg gcaagccggg atgtgcggat
    12361 ggtgcttcca tcagcgctcg cgatcctgcc agccatgcgc tggttcgaag cggtttcggg
    12421 caaagtccgc cagggcgcgc gctcggttct gccaagtgat ttcctccgcg cgctcacggg
    12481 cacggttcac ggcctccgaa taggtggcgg ggcagtcgag caggcgcatg gcttcggaaa
    12541 cgaaaccctg ctggtcatcc gccttgatcc agatgccggc gtctccgagc acctcccgca
    12601 cggatggcag cgctgtgccg agcgccggga tgccgtgcga gaggtagtcg agcgctttga
    12661 ccgggcaggt gaggtgccgg ttgtaatagg tgtcggcgag catgaccacg cccaggctgg
    12721 cgcgggcggc gagggcctcg tgcagacgcg ccggaggctg gaaaggcacg gcgtgaatcc
    12781 agtcctgaag tcctttctcc gccgcggacc gggtgagcgc ggcggcatcc ttttcataac
    12841 cgccccagaa ctcgacacgg actccgtgcg cggccaggga tgcggcgcat ccggaaagga
    12901 aggagacgcc ctttgccccg tgcatgtggc cgacataaaa gacggtgcgc ttgagccgct
    12961 tgatctcgct gtccaccggc ggcgcgggct ttgtgcccag cggaagggag atgacaggcg
    13021 tgcgcggaaa gagatgcctg tacatggccc ccatgtcgcg cgtgatgcag accaggccgc
    13081 cgagcctggg cagcagcagg cgttcgatcc agcgctcccg caggtgtttg acgcggtcta
    13141 tttcctgccg ccaagtgagg tcggcgtaaa ggtcgtgcag ttcgtaaaag cagcggatgc
    13201 gcgcgtcggc ctgcaggagc cgcatccagg gcagaaagct ggagtcgcgt gtgaagacgg
    13261 ccacgcgccc gcgcctggcg agccggcgcg cgaaggccgc ggtttgggaa tagacgcccg
    13321 ctttgccgcc gattccgggc aggagcgtgg tgtggcggta gcgcttgacg tggaaggctg
    13381 gcagcggcgg caggtcgtaa aactcgcgca ggtcatcggc ggtgtcggaa ggctggccgt
    13441 ccgcgaggtg caggtgggac tcgaatccga tggcggcgaa ggaccatgcg ttgtgcaccg
    13501 tgatgttgat gaccggcccc ggctgccgcc attcacgtcg gtttacccag agcacggtca
    13561 tgcgggatca ggcgcgggca ggggagagca tgcccacgag aaggcgctcc agcacgactt
    13621 tattgtcgag ctgcgtggtg acgagcttga gattcgcgtc gagacaggag cagagggcgc
    13681 cttcgagttc gtcgagagtg aagctggcag cctcggcgag cgcgaggaaa atgggatagg
    13741 cattgaagcc ggtgccgtcc tttttgcgcg ggatgtgcgc ggtgacggcg ggcggcagcg
    13801 cctcgaggct ggtgcagaag gcgttgtaat tgccgcggtt gacgcggtgc ttggaggcca
    13861 gttccctggc cattagcatg ctcctcacgc gcgggacgat ggcggccagg agcacgccga
    13921 cggcgttctg cccctggtag agcaggacgt cgagcaggct caaggcgcgc ggaagatcgc
    13981 gtttgccgat ggcgttgccg aggtcccaga gcacgcccgc gcggctttgc gagaccaggc
    14041 cgcgcacggt tcgtatgccg cagcggcggc gctccccaag gtagaggtcg agtttttcga
    14101 tctcgttttc gagctgtctt gtgtcgtctc ccgccatctg gacgagcagt tccagggcgc
    14161 cgctttcgaa gaccagaccc aggctgcggg cgattttttg ggcatgggcc aggacaggcc
    14221 cctcccagcc ctgcttgctg gtgtctggct tgtcgaaaat ctcgatgttt gccagcttgc
    14281 cgaggcgctt ccaggcggtg cggcgcttgt ctatggagct ggcgctgagc acgaacctca
    14341 cctccgggcc cagaccggct tcgatgacgt cgagaatgct ttcgaagccc tccaccgagt
    14401 cctgcgcttt gccggtctgg gattcgccga gaaagttggc ggcgcgcagc cacacgacct
    14461 tggctccgcc aaagaagggc agcgtctgca gcgcctggat gacctggccg caaatcctgc
    14521 cggcgtgctc ggcattgtcg gcgttgcctt cgatgatttc gttggcgaac tcgcccgcgt
    14581 ccggcggggt gagcctgcgc acgatctgca tggcggcttc cttgacgcgc gcgtcatcgg
    14641 tgccgaggac cacatgaatc tggctggcgg ctgaggcgga ttttttcggg gctggcatgg
    14701 agcgcagaga acagaggatt cgcgggacga aaagaaaatc ctccggttcc ttgtgacttc
    14761 tccgcctcat gacggcgcgg ggcggctgtg cgcggcgatg agcagggccg ttgtgccagt
    14821 gacggctcgg ttttcagcgc acggcggcca ggtcccgggc ctggccagtc cagtcctcgc
    14881 gctcgatgcg gtccttgaag gcgaggtggc gcgagatgtc ccgcacctgc tggggtgtcc
    14941 atgaggcgat gtgctcgtag cggcggacac cgagggagcg gagtttctgc tggatgccgg
    15001 gagtgatgcc gcgaatgcgg gtgagatcgt cgggcgcgag ggcggagtcg ttttcagcgc
    15061 ccgcgtctcc gccgtggatg atggaggcca gactggcgcg gcgcgccgcc ggacgcggcg
    15121 gcggcgcggc gggagagtca gtcatgagaa agctgagcgg ctcgaaggcg ggggagtccc
    15181 acgactggct gcgcgccatg gcgctgatga cgccggtttt gcgcggctgc ctgggcgcgc
    15241 cggaggcggg ggcattgttg ttggagacca gc
//
LOCUS       DAOMYJ010000118        15081 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Prosthecobacter sp. isolate AMR_MDS_5629
            NODE_1343_length_15081_cov_19.630041, whole genome shotgun
            sequence.
ACCESSION   DAOMYJ010000118 DAOMYJ010000000
VERSION     DAOMYJ010000118.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867850
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prosthecobacter sp. (wastewater metagenome)
  ORGANISM  Prosthecobacter sp.
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Verrucomicrobiaceae; Prosthecobacter.
REFERENCE   1  (bases 1 to 15081)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15081)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.30
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:34:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,742
            CDSs (total)                      :: 4,684
            Genes (coding)                    :: 4,676
            CDSs (with protein)               :: 4,676
            Genes (RNA)                       :: 58
            tRNAs                             :: 55
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15081
                     /organism="Prosthecobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1343_length_15081_cov_19.630041"
                     /isolate="AMR_MDS_5629"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1965333"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..229
                     /locus_tag="PLP58_12960"
     CDS             <1..229
                     /locus_tag="PLP58_12960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ09479.1"
                     /translation="PRVPPAAPKPPPGPPPAPKLPPPAPAAAEMEQRLRHLEKRNHAL
                     RHELHLAKLRGRMKARSARVRKRGLCTAGSA"
     gene            complement(317..1195)
                     /locus_tag="PLP58_12965"
     CDS             complement(317..1195)
                     /locus_tag="PLP58_12965"
                     /inference="COORDINATES: protein motif:HMM:NF013961.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase family protein"
                     /protein_id="HRJ09480.1"
                     /translation="MSPAALPTPALTGMTLRVRHLTRFHYEGVVTDSYNDARLCPVSD
                     PLQRCASFDLAIRPRVPVHTHHDFYLNRVDHFELHEAHENLEVEAVSVVETRPDPRGP
                     APSGIGPDALHAPGVSENYFDYVVESRFIPQSVAIWREAMDVIEGGASDLWADAVRLG
                     RHVHASFAYDPDWTHVHTDAAEALKDRRGVCQDYAHVMIALCRAKGIPARYVSGYFFN
                     DKTGDENEASHAWMEVFLPGHGWKAWDPTHGREADTRYVKLAIGRDYADAKPLSGRFR
                     GTASRHIEVSVQVRRQ"
     gene            complement(1264..2364)
                     /locus_tag="PLP58_12970"
     CDS             complement(1264..2364)
                     /locus_tag="PLP58_12970"
                     /inference="COORDINATES: protein motif:HMM:NF016086.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-E domain-containing protein"
                     /protein_id="HRJ09481.1"
                     /translation="MPPTPPPQEEVARHKQANIMLSRVAGSLYWMSRYLERAENLARL
                     VDVNLQILLDFGQVDDETLKEHWLPILRSTHDEDLFFQLYETADSESVTEFLTFRPEN
                     PNSLLSCISSARENARQVRDQISTEMWEVLNEAYHFLKGRDAVKVWQDGASEFYDQIK
                     RYSHLFQGITVSTFSRSEGFEFIQFGKYLERADKMTRLLDMKYHILLPKVTDVGGAVD
                     AAQWQAVLRSASAVEAYRRFYVADILFAKVIEFLIFQESFPRSLIFCLQQMDHFAHLI
                     ADIPQGEYRNDSERRFGRFLNNLNFTTTKDIIRQGLHEFLDEVQQEINTFGAHLYNTF
                     MFTPPVDLQAEIRFHQQQEQQQQMMMMISPAA"
     gene            complement(2407..3867)
                     /locus_tag="PLP58_12975"
     CDS             complement(2407..3867)
                     /locus_tag="PLP58_12975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007413147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="circularly permuted type 2 ATP-grasp protein"
                     /protein_id="HRJ09482.1"
                     /translation="MLFENYQPEDFFDEMFSADGAVRPHYRGVADALNEVEPAEFRNK
                     QAAVEAAFMRGGVTFTVYSDSQGTERIFPFDCVPRVISAEEWDVVEKGLIQRLTALNL
                     FLHDIYHEQKIIKDRVIPPQYLLSAKHFRREFMHFEVPKDVYVHICGTDLIRDRDGRY
                     LVLEDNLRCPSGASYMLENRAALKRTFPELFQASGVRPVSNYPGELLKVMQHCAPKNF
                     DAKDAPQCVLLTPGVFNSAYFEHAFLARQMGIPIVEGRDLVVRDFKVFMRTTAGLVKV
                     DVIYRRIDDDFLDPSVFREDSLLGVPGLVNACRAGNVSLANSIGTGVADDKVVYYFVP
                     HMIKYYLGEDPILPNVDTYLASEPKDCAHILANLDKLVVKSANEAGGYGMLMGPWASK
                     EEIEEFRAQIEANPRNFIAQNPVSLSRHPTWVGDHFEGRHIDLRPYILCGEKIVVTPG
                     GLTRVALRKGSLVVNSSQGGGSKDTWVLRHGSETGV"
     gene            complement(3963..6350)
                     /locus_tag="PLP58_12980"
     CDS             complement(3963..6350)
                     /locus_tag="PLP58_12980"
                     /inference="COORDINATES: protein motif:HMM:NF019262.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ09483.1"
                     /translation="MMKRFLFLLASTPIFAATVDYEKQLLPLLKDNCLPCHNKTTTKG
                     GLNMETVDLMLEGGDNGPSITRGDGAKSLLHQAAAGEWDSEMPPKNNKVSAVPLNSGQ
                     LALLKQWIDEGAHHAGRQERVITWEPLPAGFAPVYATAITRDARFAAAARGNQVSIYH
                     PPTARLMTRLTDDALIQSGLYQKPGVAHRDVVPALAFSPDGQRLVTGSFREVKIWRLV
                     PVNPPSNPARQAAAPGKFKFAQTASGTITLQDSASGKDLREIKHGAAIHAAALSPDGQ
                     KLATAGADHKLKLWESATGRLIREITGDLATASQLSAATFTADRTALEAAWQTESVKK
                     AEKEAADLNARLTKGKELETSAQKTLEDKRRDMEAKTAAKAKAGADLKALESPPPQPT
                     PASENEAAPAPAKPGEAAAKKLAEARESAAKADTEAKAAQEALKRAETAVQDAAQEIK
                     LVTGLIATAGDAVKAAKAALEKSRKEQEAAAAAKAVSAKSDAEAVRGVLALVFSPDGS
                     QIASLDTQNHIRLWSVETGMALASSALKMTLAWPDARGPVAGSSRVQFLTSNEAMQWK
                     LERVLGSGDAKSPVTDRVNALAFSPDGKTLAVGSGEPSRSGDISLWNMGDGALKARWD
                     EAHLDSVLALEFSPDGRLLASGGADKALRVFDAATGNLVKNLEGHTHHVLGVSWRADG
                     RLLASAGADAVVKIWDWTTGERRQNITGWDKEVTAVRWLGGTDTLATTSGDGKVRVLT
                     SAGAELRSLPGAQDFMNTLCTTAEGSVLAAGGDDGTLRLWHLPTAKETGVFAVTR"
     gene            complement(6357..8393)
                     /locus_tag="PLP58_12985"
     CDS             complement(6357..8393)
                     /locus_tag="PLP58_12985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ09484.1"
                     /translation="MKLLPLFILTPALALAQALNFPTPVITSIHPLGGQPGKTLDLTL
                     KGADLNGLRGVHFSPDPGAPVEAKPKLDAKGQPVANTLTLALPAEVKSGLYELRVIGD
                     FGLSNPRVFQISAQPVADSPGTNDKPETALKITPDTAAHGVFKSAAPHWFEFTGKAGQ
                     RVFAAFDGAAFDVRTRLNGSLHDASGRETARLRDGWLAATLPANGVYRLKVHDLMHGT
                     GDDFAYRLILTTGPVVYSSHHSPGDESRILHGWNLPGSETLPSLQVRPGPPLERLVTD
                     TTNAEKLIASSPLPVFHFDGETEPASAANGQPAALSIGQTRGGWFPAHGSAHVYDLAF
                     KKGDRFVIEVISALHGQPTDPHLLIENLKKDTAGAGTLTPQAEVNDMPALVPAPSVAR
                     VPLLDPAYAFEAKADGVFRISISDPLNAANGRRHPYTLRVRSLDDPDTRAAIAMHALP
                     PRAAATGPHVIAPANLWRGGISVIEVALPLRTALSGPVEITTRVPLPAGVTSLGGFAG
                     KGQRMACLAFQAAGDAPPGHAILTDLPPALHLTRTVADTNREFLFTRLGGPPVLAVVD
                     QPAPARLELPAITEVKAGEKVEIIVKAHRAEACTEALVLKVIGLGDPAKAPAVTIPAK
                     AAEARLVVDAKALALAPGEHGFILQGPAVMPVGKNAPKPASFLVHSNPARLRVK"
     gene            complement(8461..10662)
                     /locus_tag="PLP58_12990"
     CDS             complement(8461..10662)
                     /locus_tag="PLP58_12990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009962806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1549 and DUF1553 domain-containing protein"
                     /protein_id="HRJ09485.1"
                     /translation="MRNPSCVIPLAALCLSLPAFAEEKQRRLNFANDIVPILTKGGCN
                     SGGCHGKASGQNGFKLSLLGFEPQEDYEHIVKEARGRRIFPAAPEQSLLLTKGTAQLP
                     HGGGKKLDPESEDYQDLVRWIREGMPYGSPDDPKMARISVEPAALTLPLKARQQLKVL
                     AHYTDGSTRDVTRRALYEANEKSMAETTETGEVQLLDLPGDVAVMVRYQGKVATFRAT
                     VPLGAPVESLPPESNFVDRHVFAKLQTIGMPPSPLADDATFLRRVTLDLTGRLPTVDE
                     MKDFLASKEAGKRAAKVDQFLDSTEYAEYFANKWSALLRNQRTQPLYARGTYLFWSWL
                     RDAMADNMPFDQFARQVIAASGSLDQHPPTAWYRQVKEPKQQMEDVAQLFLGTRMQCA
                     QCHHHPFEKWSQEDYYGLAAFFANVARKPAGDRYEELIYHKRGTAQMTNIRTKQPVMP
                     TSLGAESVKLKPEQDPRHVLADWMSAKDNPFFAHTLVNRYWKHFFNRGLVEPEDDMRE
                     TNPPVNPELLHALAADFVESGYDMKRLIRTITTSTTYQLSAVPNEHNEKDRQNFSRFY
                     PKRLTAEVLYDAVNTLLEAESKFAGQAPGTRAVALPDNSYNQTTYFLTVFGRPDSSSA
                     CECERTQEASLAQSLHLLNAADIQTQLSREGGRADKLAKDNRPDDDKVGDLYHIALSR
                     DPNAREMKLALSHLAKKTEGKTGDALPVARREAFEDILWALLNTKEFLFNH"
     gene            10781..12199
                     /locus_tag="PLP58_12995"
     CDS             10781..12199
                     /locus_tag="PLP58_12995"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arylsulfatase"
                     /protein_id="HRJ09486.1"
                     /translation="MHRILAPFLALVLCCESSAAKPNLIWIMADDLGWGDLGCYGQKV
                     ITTPNLDRMAKEGLRFTHFYAGATVCAPSRSVLMTGLHHGHTRVRGNAGQQNPAAQAL
                     KPGDVTVASVLKKAGYQTALIGKWGLGNAGAAESGLPRKHGFDYFYGYLSQHHAHNHF
                     PDYLWRNETKEPLSNVIVPVGEDGAGYATEAVHYADDLFADDALKFVSENKARPFFLY
                     WSMVIPHANNERGRILGDGAHVPDYGPYADKDWPNQDKGQAAMITRLDGYVGRMMEHL
                     KSLGLAENTLVIFTSDNGPHNESKHDLERFDPNGPYTGIKRSLTDGGIRVPFIAWWPG
                     KVKPGESAHTGYFPDFMPTAAELAGAPAPEKTDGISLVPVLTGRADEQKKHEFLYWEF
                     HEGGFKQAALYQGRWKGIRMGGPDAPVQLFDQQNDVAEETNVADKHPDIAAKIGAYLK
                     TARTPLDEWEPRWQAPGKKGGK"
     gene            12287..14236
                     /gene="speA"
                     /locus_tag="PLP58_13000"
     CDS             12287..14236
                     /gene="speA"
                     /locus_tag="PLP58_13000"
                     /EC_number="4.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009962444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008792 - arginine decarboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biosynthetic arginine decarboxylase"
                     /protein_id="HRJ09487.1"
                     /translation="MQRRPPSKAPAWTVQESAALYGIREWGHGYFDVSAKGEVVVNLK
                     DGKKTKPVSIAEIVKGLRERGTQLPVLIRFGDLLRWRIDELNEGFATAIREGKYQGVY
                     RGVYPIKVNQQQEVIDEITRYGRKYHYGLEAGSKPELIAALAHMHDPEAYIVCNGYKD
                     EEFIDLALYAQKMGLQVILVLEMPSELALILERSRKMGVRPTLGVRFRLSAESAGHWS
                     GSGGDASVFGLNISQLMGVVDQLREHGMLDCLRMLHYHQGSQIPNIRAIRQAVTEATR
                     VYCGLVHEGARMGILDLGGGLAISYDGFKGATSASSNYGTKEYCADVIEAITEVTAEA
                     GVPHPDLITESGRAIVAYYSVLVINILDVNRFEPSRSGFELDKDSPQLLRNLADLHQE
                     RSKDPAKLTRDRVQEIYNDAVYYRDKLRSEFNYGKVALRERAQGEEMYWDIMTWISGK
                     LDSVGHDGSQMERMSTVMTDYYYGNFSVFQSLPDLWAIDQIFPVMPIHRLKEKPTRNA
                     VLSDITCDSDGKIARFAHSGEICGSLPLHDPDFEKNGDYMLGIFLVGAYQETLGDLHN
                     LLGDTNVVSVSLEGGKLKYRREQEGDSVSEVLSYVEYDPKDLANRFRNLAESAVVSKR
                     ITAAERREIMGAYDAGLRGYTYFET"
     gene            complement(14639..>15081)
                     /gene="folK"
                     /locus_tag="PLP58_13005"
     CDS             complement(14639..>15081)
                     /gene="folK"
                     /locus_tag="PLP58_13005"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR01498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003848 -
                     2-amino-4-hydroxy-6-hydroxymethyldihydropteridine
                     diphosphokinase activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HRJ09488.1"
                     /translation="ELLLARLPESARLTARAGWYETEPVDCAPGTQAFLNSVIEIEAD
                     LSPAEMHAHLQAVEQAMGRPAVREKNAPRTLDLDLLYAGGAVSADPALLLPHPRLHQR
                     RFVLAPLAEARPRLVLPGFEQSVSGLLAALPDDPSGVRFMGPWR"
BASE COUNT         2558 a         4594 c         5251 g         2678 t
ORIGIN      
        1 gcccagagtc ccgcccgccg ctccgaagcc tcctcccggc ccgccgcctg cgccgaagct
       61 gcccccgccc gcgcccgccg cggcggagat ggagcaaagg ctgcgccatc tggagaagcg
      121 caaccacgcg ctgcggcacg agttgcatct ggcgaagctg cgcggacgca tgaaggcgcg
      181 ctccgcccgc gtgaggaagc gcgggctttg cacggctggc tcagcctgaa atgcggacgg
      241 gcgcggattt ttaacgactc tgaagccgcc cccagcccct tgaacccgcg tcgtccgcgc
      301 ggacaggatg caaggctcac tgcctgcgca cctgcacgct gacctcaatg tgcctgctgg
      361 cggtgccgcg gaagcggccg ctcaatggct tggcatccgc gtagtcgcgg ccgatggcga
      421 gcttcacata acgggtgtcg gcctcgcggc cgtgggtggg atcccaggct ttccagccgt
      481 gccccggcag gaagacctcc atccaggcgt gggaggcctc gttttcatcg ccggtcttgt
      541 cattgaaaaa gtagccgctg acgtaacgcg ctggaatgcc cttggcgcgg cagagggcga
      601 tcatcacatg cgcgtagtcc tggcacacgc cgcggcggtc tttgagcgcc tccgcggcgt
      661 ccgtgtggac gtgcgtccag tccgggtcgt aggcgaagct ggcgtggaca tggcggccga
      721 gcctgacggc atcggcccag aggtcggaag cgccgccctc gatgacatcc atggcctcgc
      781 gccagatggc gacgctctgc gggatgaagc gggactcgac gacataatca aagtagttct
      841 cgctgacgcc gggcgcgtgc agggcatcgg gtccgatgcc ggacggggcc gggccgcgcg
      901 ggtcgggccg ggtctcgacg acggagacgg cctcgacctc aagattctca tgagcctcgt
      961 gcagctcaaa gtggtccacc cggttcagat aaaagtcatg atgggtgtgg acgggcacgc
     1021 gcgggcggat ggcgaggtca aaggaggcgc agcgctggag cggatcggag acggggcaga
     1081 gacgcgcgtc gttgtagctg tcggtgacga cgccctcgta atgaaaacgg gtgaggtgac
     1141 gcacgcgcag cgtcatgccg gtgagggcgg gcgtcgggag ggcggctggg gacaattcgg
     1201 ttggctgggt ttgtcgtcag aatgaaaggg aatcacggga atgcagcccg ttttttcact
     1261 ccctcaggcc gcgggcgaaa tcatcatcat catctgctgc tgctgctcct gctgctggtg
     1321 gaagcgtatc tccgcctgca aatccacggg cggggtgaac atgaaggtgt tgtacaagtg
     1381 cgcgccgaat gtgttgatct cctgctgcac ctcgtcgagg aactcgtgca ggccctggcg
     1441 gatgatgtcc ttcgtggtgg tgaagttcag gttgttgagg aagcggccaa agcgccgctc
     1501 ggagtcgttg cggtactcgc cctgcgggat gtcggcgatg agatgggcga agtggtccat
     1561 ctgctggagg cagaagatga gggagcgcgg gaagctttcc tggaaaatga ggaactcgat
     1621 gaccttggcg aagaggatgt ccgccacata aaagcggcgg taggcctcca ccgcgctggc
     1681 ggagcgcagc acggcctgcc actgcgccgc gtccacggcg ccgccgacgt cggtgacctt
     1741 cggcagcagg atgtggtact tcatgtcgag caggcgggtc attttgtccg cgcgctcgag
     1801 gtatttgccg aactggatga actcgaagcc ctcgctgcgc gagaaggtgg agacggtgat
     1861 gccctggaag agatgggagt agcgtttgat ctggtcgtag aactcgctgg cgccgtcctg
     1921 ccagaccttc acggcgtcgc ggcctttgag gaagtggtag gcctcgttga gcacctccca
     1981 catttcggtg gagatctggt cgcgcacctg gcgggcgttt tccctggcgc tggagatgca
     2041 ggagaggagc gagtttggat tttcgggacg gaaggtgagg aactcggtga cgctctcgct
     2101 gtcggcggtc tcgtagagct ggaagaacag atcctcgtca tgggtggagc gcaggatggg
     2161 cagccagtgc tcctttagcg tctcgtcatc cacctggccg aaatcgagca ggatctgcag
     2221 gttcacatcc acgaggcgcg cgaggttttc cgcgcgctcc aggtagcggc tcatccagta
     2281 caagctgccg gccacgcggg agagcatgat gtttgcctgc ttgtgccggg cgacttcttc
     2341 ttggggcgga ggggtgggtg gcatgggagt gtctcggttc gcggttggcg gtgactggag
     2401 atgaaatcaa acaccggtct ccgagccgtg ccgcagcacc caggtgtcct tcgagccgcc
     2461 gccctgggag ctgttgacga cgagggagcc cttgcgcagc gccacgcggg tgagaccgcc
     2521 gggggtgacg acgatttttt ccccgcacag aatgtagggc cggaggtcaa tgtggcggcc
     2581 ctcgaaatga tcgcccaccc aggtggggtg gcggctcaga gagacggggt tttgcgcgat
     2641 gaagttgcgc gggttggcct cgatctgcgc gcggaactcc tcgatctcct ccttgctggc
     2701 ccagggtccc atgagcatgc catagccgcc ggcctcgttg gcggacttca ccaccagctt
     2761 gtcgagattg gccaggatgt gcgcgcagtc cttcggctcg gaggcgaggt aggtgtccac
     2821 gttcggcagg atggggtcct cgccgaggta gtatttgatc atgtgcggga cgaagtaata
     2881 gaccaccttg tcatccgcca caccggtgcc gatgctgttg gccaggctga catttcctgc
     2941 gcggcaggca ttcaccaggc cgggcacgcc gagcagcgag tcctcgcgga agacggaggg
     3001 gtcgaggaag tcgtcatcaa ttcggcggta gatgacatcc accttcacca gcccggcggt
     3061 ggtgcgcatg aagaccttga agtcgcgcac cacgaggtcg cgcccctcga cgatggggat
     3121 gcccatctgg cgcgccagga aggcgtgctc gaagtaggcg ctgttgaaca cgcccggcgt
     3181 cagcagcacg cactgcggcg cgtccttggc gtcgaagttt ttcggggcgc agtgctgcat
     3241 taccttgagc agctcgccgg ggtagttgct caccggacgc acgccgctgg cctggaacag
     3301 ctcggggaag gtgcgcttca gggcggcgcg attttccagc atgtagctgg cgccggaggg
     3361 gcagcgcaga ttgtcctcaa gcacgaggta gcgcccgtcc ctgtcacgaa tcaaatccgt
     3421 gccgcagatg tggacataaa cgtccttggg cacctcgaag tgcatgaact cgcgccggaa
     3481 gtgtttcgcg ctcagcagat actgcggcgg gatgacgcgg tccttgatga tcttctgctc
     3541 gtggtagatg tcgtgcagga aaaggttcag ggcggtgagc ctttggatga gccccttctc
     3601 caccacgtcc cactcctcgg cggagatgac gcgcggcacg cagtcgaagg ggaagatgcg
     3661 ctcggtgccc tgggagtcgg agtacacggt gaaggtgacg ccgccgcgca tgaaggcagc
     3721 ttccaccgcc gcctgcttgt tgcggaactc ggcgggttcg acctcgttca gggcatcggc
     3781 cactcctcgg taatgcggcc gcacggcccc gtcggcggag aacatttcat cgaaaaaatc
     3841 ctccggctgg tagttttcga aaagcatgtc ggtcggtcgc taaaaggttg gtggtgtttg
     3901 cataagcagg actggggcca acttccagcg gggaattcaa aggccgccac accaccccag
     3961 gctcagcgcg tgacagcaaa cacgccggtc tctttcgcgg tcgggagatg ccagaggcgc
     4021 agggtgccgt catcaccgcc cgctgccagg acggaacctt cggcggtggt gcagagggtg
     4081 ttcatgaaat cctgcgcgcc gggcagcgac ctcagctcag cgccagcgct ggtgagcacg
     4141 cgcactttgc cgtcgccgga ggtggtggcg agggtgtcgg tgccgccgag ccagcgcacg
     4201 gcggtgactt ccttgtccca tccggtgatg ttttggcgcc gctcgccggt ggtccagtcc
     4261 cagattttca ccaccgcgtc cgcgccagcg ctggcgagca ggcggccatc ggcgcgccag
     4321 gagacgccga ggacgtgatg ggtgtggcct tcgaggtttt tcaccagatt gccggtggcg
     4381 gcgtcaaaca cgcgcagggc tttgtccgcg ccgccgctgg cgagcagcct gccgtcagga
     4441 gagaactcga gcgcgagaac gctgtcgaga tgcgcctcat cccagcgcgc cttcaatgcg
     4501 ccatcgccca tgttccacaa gctgatgtct ccggagcggg agggttcgcc gctgccgacg
     4561 gcgagcgttt tgccatcggg ggaaaaggcg agggcattga cgcggtcggt gacgggtgac
     4621 ttggcatcgc cgctgccgag gacgcgttcg agcttccact gcatggcctc gttggaagtg
     4681 agaaactgaa cgcgggatga acccgccacg gggccgcgcg cgtcaggcca tgcgagtgtc
     4741 attttcagcg ccgacgaagc cagtgccatg cctgtttcca ctgaccagag gcggatgtgg
     4801 ttttgcgtgt cgagggatgc gatttgggat ccatcgggcg agaagacgag ggcaagcaca
     4861 cctctgacgg cctcagcgtc gctcttggcc gaaacggctt tggcggctgc ggcggcttcc
     4921 tgctccttgc ggcttttctc cagggctgcc ttggcggctt tcacagcatc tccggcagtg
     4981 gcgatgaggc cggtgacgag tttgatctcc tgggccgcgt cctgcacggc ggtctcggcg
     5041 cgcttgaggg cttcctgcgc ggctttggct tcggtgtccg ctttggctgc ggactcgcgg
     5101 gcttcggcga gttttttggc ggcggcttcg ccgggcttgg cgggggcggg agcggcttcg
     5161 ttttcggagg ctggggtcgg ctggggtggc ggtgattcca gggctttcaa gtctgcgccg
     5221 gctttggctt tggccgctgt tttggcttcc atatccctgc gtttgtcttc gagggttttt
     5281 tgggcgctgg tttcgagttc cttgcctttg gtcaggcggg cgttgaggtc ggcggcttct
     5341 ttttcagcct tcttcacgct ctcagtttgc caggcagcct caagcgcggt gcggtcggcg
     5401 gtgaaggtgg cggcgctgag ctggctggcg gtggccaggt caccggtgat ctcgcggatg
     5461 agcctgccgg tggcggattc ccagagcttg agtttatggt ccgcgccggc ggtggcgagt
     5521 ttttggccgt cgggactgag cgctgcggca tggatggccg cgccatgctt gatctcacgc
     5581 aggtctttgc cgcttgcgct gtcctgaagc gtgatcgtcc cggaggcggt ttgagcaaac
     5641 ttgaacttgc ccggcgcggc ggcctggcgc gctggatttg acggcgggtt gacgggcacg
     5701 agccgccaga ttttgacctc gcggaagctg ccggtgacaa gtcgctggcc gtccgggctg
     5761 aaggcgagcg cgggcacgac atcgcggtgc gccacgccgg gcttttgata gaggccgctt
     5821 tggatcaggg cgtcgtcggt gaggcgggtc atgagccgcg cggtgggcgg atgataaatc
     5881 gagacctggt tgccgcgcgc ggcggcggcg aagcgcgcgt cgcgcgtgat ggcggtggca
     5941 tagacgggcg cgaatccggc gggcagaggc tcccaggtga tgacgcgctc ctgcctgccg
     6001 gcgtggtgcg cgccctcgtc aatccattgc ttgagcaggg cgagctggcc gctgttaagc
     6061 ggcacggcgc tgaccttgtt gttcttcggc ggcatctcgg agtcccactc gccggcggcg
     6121 gcctggtgaa gcaggctttt ggcgccatcg ccgcgcgtga tggaggggcc gttgtcgccg
     6181 ccttcgagca tgaggtccac ggtctccatg ttgaggccgc ccttggtggt ggtcttgttg
     6241 tggcagggca ggcagttgtc cttcagcagg ggcagcaact gcttttcata atccaccgtg
     6301 gcggcgaaga tgggcgtgga ggcgaggaga aaaagaaaac gcttcatcat ggcggatcac
     6361 ttcacgcgca gacgcgcggg gttcgagtgg acgaggaagg aggcgggctt gggcgcgttt
     6421 ttgccaactg gcatgacagc cgggccttgc aggatgaagc catgctcgcc aggggcaagc
     6481 gcgagcgcct tggcatcaac cacgagcctg gcctccgcgg ctttggcggg gatggtgacg
     6541 gcgggggctt tggcgggatc accaaggccg ataactttta aaaccagcgc ctcggtgcag
     6601 gcttcggcgc ggtgcgcttt gacaatgatc tcgacttttt caccggcttt gacctcggtg
     6661 atcgcaggca actccaaacg ggcgggcgct ggctggtcca caaccgcgag cacgggaggg
     6721 ccgccgaggc gggtgaaaag gaactcgcga ttggtgtcgg ccacagtgcg ggtcagatgc
     6781 agcgcgggtg gcaaatcggt gaggatggca tggccaggag gagcgtcgcc ggctgcttga
     6841 aaggccagac aggccatgcg ctggcctttg ccggcgaagc cgccgaggct ggtgacgccg
     6901 gcgggcaatg gcacgcgggt ggtgatctcc accgggccgc tgagcgcggt gcgcaggggc
     6961 aaggccacct cgatgacact gatgccgccg cgccagagat tggcgggcgc gatgacatgc
     7021 ggccctgtgg ctgcggcgcg cggcggcagg gcgtgcatgg cgatggcggc ccgggtgtct
     7081 gggtcatcca gggagcggac gcgcaaggtg tagggatggc ggcggccatt ggcggcattg
     7141 agcggatcac tgatggaaat gcggaagacg ccgtcggctt tggcctcgaa agcataggcg
     7201 gggtcgagca agggcacgcg cgcgacggat ggcgcgggca ccaacgctgg catgtcgttg
     7261 acctcagcct gcggggtgag tgtcccggcg cctgcggtgt cttttttgag gttctcgatg
     7321 agcagatgcg gatcggtggg ctggccgtga agcgcggaga tgacctcgat gacaaagcgg
     7381 tcgccctttt tgaaggcgag gtcatagacg tgcgcgctgc catgagcggg gaaccatccg
     7441 ccgcgggtct ggccgatgct caatgccgca ggctggccgt tggccgcgga cgcgggttca
     7501 gtttcgccgt caaagtggaa gacgggcagc ggactgctgg cgatgagttt ttcggcgttt
     7561 gtggtgtcgg tgacgagtcg ctccagtggc gggccggggc ggacttgcag agacgggagc
     7621 gtttcgcttc cgggcagatt ccagccgtgg agaatgcggg actcatcacc cggagagtga
     7681 tggctgctgt acacgaccgg gccggtggtg agaatgaggc ggtaggcgaa gtcgtcgccg
     7741 gtgccgtgca tgaggtcgtg gactttgagc cggtaaacac cgttggcggg caatgtggcg
     7801 gccagccagc cgtcgcgcag acgcgcagtc tcgcgtccgc tggcgtcgtg caggctgccg
     7861 ttgagccgcg tgcggacatc gaaggcggct ccatcaaagg cggcaaacac gcgctggcct
     7921 gccttgccgg tgaactcgaa ccaatgcggc gcggcgcttt tgaagacgcc atgcgcggcg
     7981 gtgtcgggcg tgattttcaa agcggtttcc ggcttgtcat tcgtgcctgg cgagtccgcc
     8041 acgggctgcg cgctgatttg aaacacacgc ggattggaga ggccgaagtc gccaatgaca
     8101 cgcagctcat acagaccgga cttgacctcg gcgggcaggg cgagggtgag tgtgtttgcc
     8161 accggctggc ctttggcgtc gagtttgggt ttggcctcaa cgggcgcgcc ggggtcgggt
     8221 gagaaatgca cgccgcgcag gccattgaga tcggcgcctt tgagagtgag gtccagcgtc
     8281 tttcccggct ggccgccgag gggatgaatg gaggtgatga ccggtgtggg gaagttcagc
     8341 gcctgggcaa gcgcgagcgc gggagtcaga atgaaaagcg ggagcagctt catgaatggg
     8401 tatggagaac aggcgtcccg cctgttccgg gcacaggcgg gacgcccgtg ccccatgctg
     8461 tcagtggttg aagaggaatt ccttggtgtt cagcagcgcc cataggatgt cctcaaaggc
     8521 ctcgcggcgc gcgacgggca gggcgtcgcc ggttttgcct tcggtctttt tggcgaggtg
     8581 ggagagcgcg agcttcattt cccgcgcgtt cgggtcgcgg ctgagggcga tgtgataaag
     8641 gtcgcccact ttgtcatcat cgggccggtt gtctttggcg agcttgtcgg cgcggccgcc
     8701 ttcacgcgag agctgggtct ggatgtcggc ggcgttgagc aggtgcaggc tctgcgccag
     8761 gctggcctcc tgggtgcgct cgcactcgca ggcggaggag gagtcggggc ggccgaagac
     8821 ggtgaggaaa taggtggtct ggttgtagct gttgtccggc agggccacgg cgcgggtgcc
     8881 gggcgcctgc ccggcgaatt tgctctcggc ttcgagcagg gtgttcaccg cgtcatacag
     8941 cacctcggcg gtgaggcgct tgggatagaa gcggctgaaa ttctgccggt ccttctcgtt
     9001 gtgctcgttc ggaacggcgc tgagctggta ggtggtggag gtggtgatgg tgcggatgag
     9061 gcgcttcatg tcatagccgc tctccacgaa atcggctgcc agagcgtgca gcagttcagg
     9121 gttcacgggc gggttcgtct cgcgcatgtc gtcctccggc tcgaccaggc cgcgattgaa
     9181 aaaatgcttc cagtagcggt tcacgagcgt gtgggcgaag aaggggttgt ccttcgcgct
     9241 catccaatcg gcgaggacgt gcctcgggtc ctgctcgggc ttcagcttca cgctttccgc
     9301 gccgaggctg gtgggcatga cgggctgctt ggtgcgtatg ttggtcatct gcgcggtgcc
     9361 gcgcttgtga taaatcagct cctcatagcg gtcgcccgcg ggcttgcgcg cgacgttggc
     9421 gaaaaaggcg gccagcccgt agtagtcctc ctggctccat ttttcaaagg ggtggtgatg
     9481 gcactgcgcg cactgcatgc gcgtgccgag gaagagctga gccacgtcct ccatctgctg
     9541 cttcggctcc ttcacctggc gataccacgc ggtgggcgga tgctgatcca ggctgcccga
     9601 ggcggcgatg acctggcgcg cgaactggtc gaagggcatg ttgtcggcca tcgcatcgcg
     9661 cagccaggac cagaaaagat aagtgccgcg cgcgtagagc ggctgggtgc gctggttgcg
     9721 cagcagggcg ctccatttgt tggcgaagta ttcggcgtat tcggtggagt cgaggaactg
     9781 gtccaccttc gctgcgcgtt tgcctgcctc tttggaggcg aggaaatcct tcatttcatc
     9841 cacggtcggc aggcgtccgg tgaggtctag cgtcacgcgg cggaggaaag tggcgtcatc
     9901 ggccaagggc gagggcggca tgccgatggt ctgcaatttg gcaaagacat gccggtccac
     9961 gaagttgctc tcaggcggca aagactccac cggcgcgccc agcggcacgg tggcgcggaa
    10021 ggtggccacc ttgccctggt agcgcaccat cacggccaca tcaccgggca ggtcgaggag
    10081 ttgcacttca ccggtttcgg tagtctcggc catggacttt tcattggcct cgtaaagcgc
    10141 gcgccgggtc acgtcgcgtg tggagccgtc ggtgtaatgg gcgagcactt tgagctgctg
    10201 gcgggctttg agcggcaggg tcaaagcggc gggctcgacg ctgatgcggg ccattttggg
    10261 atcatccggg ctgccataag gcatgccttc gcggatccag cgcacgaggt cctgatagtc
    10321 ctcggattcg ggatcgagct tcttgccgcc accatgcggg agctgggcgg tgcctttggt
    10381 gagcagcagg ctttgctccg gcgcggcggg gaagatgcgg cgcccgcgcg cctccttgac
    10441 gatgtgctcg tagtcctcct gcggctcgaa gccgagcagg gagagcttga agccgttttg
    10501 tccgctggcc ttgccatggc agccgccgct gttgcagccg cccttggtga ggatgggcac
    10561 gatgtcgttg gcgaagttca gccggcgctg tttctcctcc gcaaaagcgg gcagtgagag
    10621 acacagggcg gccaggggga tgacgcatga cgggtttcgc atggcgagaa cctacggctg
    10681 caatggcggg attcttctcg gcggacaatg acgcgcgcag tgcccggcat ggacaagcat
    10741 ggcaagacgg cccgcctttt ctcgttccca tccgcccgtc atgcatcgaa ttctcgcccc
    10801 cttcctcgcc cttgtccttt gctgcgaatc ctccgccgcc aaacccaatc tcatctggat
    10861 catggcggat gatctgggct ggggcgatct cggctgctat gggcaaaaag tcatcaccac
    10921 gccgaatctc gaccgcatgg cgaaggaggg cctgcgtttc acgcactttt acgcgggcgc
    10981 gacggtctgc gcgccttcac gcagcgtgct catgacgggc ctgcaccacg gccacacccg
    11041 cgtgcggggc aacgcggggc agcaaaaccc ggcggcgcag gctttgaagc ccggagacgt
    11101 gaccgtggca tccgttttga agaaagcagg ctaccagacg gcgctcatcg gcaaatgggg
    11161 cctcgggaat gccggcgcgg cggagagcgg cctgccgcgc aaacatggct tcgactactt
    11221 ttacggctac ctcagccagc atcacgcgca caaccacttc cccgactatc tctggcgcaa
    11281 tgagaccaag gagccgctct ccaatgtcat cgtgccggtc ggcgaggatg gcgcgggcta
    11341 cgccacggag gcggttcatt acgcggacga tctttttgcc gatgacgcgc tgaaattcgt
    11401 cagcgagaac aaggcgcggc cctttttcct ctactggagc atggtgatcc cgcatgcgaa
    11461 caacgagcgc gggcgcatcc tgggcgacgg cgcgcatgtg cccgactacg gaccctatgc
    11521 ggacaaggac tggccgaacc aggacaaggg ccaggcggcg atgatcacgc gcctcgacgg
    11581 ctatgtgggc cgcatgatgg agcatctgaa gtcgctcggc ctcgcggaga acacgctcgt
    11641 catcttcacc agcgacaacg gcccgcacaa tgagagcaag cacgacctgg agcgcttcga
    11701 ccccaacggc ccctacaccg gcatcaagcg cagcctcacg gacggcggca tccgcgtgcc
    11761 cttcatcgcc tggtggcctg gaaaagtgaa gccgggcgag tccgcgcaca ccggctactt
    11821 tcccgacttc atgcccaccg ccgccgaact cgccggagcg cccgcgccgg agaagacgga
    11881 cggcatcagc ctcgtgcctg tgctcaccgg cagggcggac gagcagaaga agcacgagtt
    11941 tctctactgg gagtttcacg agggcggctt caagcaggcc gcgctgtatc aaggccgttg
    12001 gaaaggcatc cgcatgggcg gcccggacgc gcccgtgcag ctctttgacc agcaaaacga
    12061 cgtggcggag gaaaccaacg tcgcggacaa gcaccctgac atcgccgcga agatcggcgc
    12121 gtatctgaaa acagcccgca cgcctctcga cgaatgggag ccgcgctggc aggcccccgg
    12181 aaagaaaggc ggcaagtgag cctcggcgcg cgcgggccgg agcgcgcggc atgtttcact
    12241 tttcatcccg gagattgcgc ccatactcgc gcccctccgc ctgaacatgc aaagacgccc
    12301 tccttccaaa gcccccgcct ggaccgtcca ggaaagcgcc gccctttacg gcatccgtga
    12361 atggggccac ggctacttcg acgtgtccgc gaagggcgag gtggtggtca acctcaagga
    12421 cggcaagaag accaagccgg tctccatcgc cgagatcgtc aaaggattgc gcgagcgcgg
    12481 cacgcagctc cccgtgctca tccgttttgg cgacctgctg cgctggcgca ttgacgagct
    12541 caacgagggc ttcgccaccg ccatccgcga gggcaaatac cagggcgtgt accgcggtgt
    12601 ctatcccatc aaggtgaacc agcagcagga ggtcattgac gagatcaccc gctacgggcg
    12661 caaataccac tacgggctgg aggccggcag caagccggag ctcatcgccg cgctcgcgca
    12721 catgcacgac ccggaggcct acatcgtctg caacggctac aaggacgagg agttcattga
    12781 cctggcgctc tacgcgcaaa agatgggcct ccaggtcatc ctcgtgctgg agatgcccag
    12841 cgagctggcc ttgatcctgg agcgctcgcg caaaatgggc gtgcggccca cgctcggcgt
    12901 gcgcttccgc ctcagcgccg agagcgccgg gcactggagc ggctccggcg gggacgccag
    12961 cgtcttcggg ctgaacatca gccagctcat gggcgtggtg gaccagttgc gcgagcacgg
    13021 catgctcgac tgcctgcgca tgctgcacta ccaccagggc tcgcagatcc cgaacatccg
    13081 cgccatccgc caggccgtca ccgaggccac gcgcgtgtat tgcggcctcg tccacgaagg
    13141 cgcgcgcatg ggcatcctgg acctcggcgg cggcctcgcc atttcctatg acggcttcaa
    13201 gggcgccacc tccgcctcca gcaactacgg caccaaggaa tactgcgccg acgtcattga
    13261 ggccatcacc gaggtcaccg ccgaggccgg tgtgccgcac ccggacctca tcaccgagtc
    13321 cggccgcgcc atcgtggcct actactccgt gctggtcatc aacatcctgg atgtgaaccg
    13381 cttcgaaccc tcccgctccg gcttcgaact cgacaaggac tccccgcaac tgctgcgcaa
    13441 cctggccgac ctccaccagg agcgctcgaa ggaccccgcc aagctcaccc gcgaccgcgt
    13501 gcaggagatc tacaacgacg ccgtctatta ccgcgacaag ctgcgcagcg agttcaacta
    13561 cggaaaggtc gccctgcgcg agcgcgccca gggcgaggag atgtactggg acatcatgac
    13621 ctggatctcc ggcaagctcg actccgtggg ccacgacggc agccagatgg agcgcatgag
    13681 caccgtgatg accgactact attacggcaa cttcagcgtc ttccaaagcc tgccggacct
    13741 ctgggccatt gaccagatct tccccgtcat gcccatccac cggctcaagg aaaagcccac
    13801 gcgcaatgcc gtgctctccg acatcacctg cgacagcgac ggaaagatcg cccgcttcgc
    13861 gcacagcggc gagatttgcg gcagcctgcc gctgcacgac ccggacttcg aaaaaaacgg
    13921 cgactacatg ctcggcatct tcctcgtcgg cgcctaccag gagaccctcg gcgacctgca
    13981 caacctgctc ggtgacacaa acgtggtcag cgtcagcctg gagggcggca agctcaaata
    14041 ccgccgcgag caggagggtg acagcgtctc cgaggtgctc agctatgtcg agtacgaccc
    14101 caaggacctc gccaaccgct tccgcaacct ggcagagagc gccgtcgtct ccaaacgcat
    14161 caccgccgcc gagcgccgcg agatcatggg cgcctacgat gccggtctgc gcggctacac
    14221 ttatttcgag acctgagcgc gctgtccccg atgaccgggg accgggctcc gatgaaaagg
    14281 gacgcgtccc cgccgggccg ggacagggcc ccgacagccg gggaccggtc cccgatgaaa
    14341 agggacgcgt ccccgccggg ccgggacagg gccccgacag ccggggaccg gtccccgatg
    14401 aaaagggacg cgtccccgcc gggccgggac agggccccga cgaccgggga ccggtccccg
    14461 atgaacaggg acgcgtcccc gccgggccgg gacagggccc cgacgaccgg ggaccagtcc
    14521 ccgatgaaca gggacgcgtg ctccacagga taagacaagt cccccatggc cggggacacc
    14581 gctttggccg cctgcctccg cggagcggga gtccagcgcg ggcgaaacac ccccaaactc
    14641 accgccacgg ccccatgaaa cgcaccccgg agggatcatc cggcagcgcc gccagcagcc
    14701 cgctcacgct ttgttcaaag cccggcagca ccagccgcgg gcgcgcctcg gccagcggcg
    14761 ccagcacaaa gcggcgctga tgcaggcgcg ggtgcggcag cagcagcgcg gggtccgcgc
    14821 tgaccgcgcc gcccgcgtag agcaaatcca gatccagcgt gcgcggcgcg tttttctccc
    14881 gcaccgccgg gcggcccatg gcctgctcca ccgcctgcaa atgcgcgtgc atctccgccg
    14941 gggagaggtc cgcctcaatc tcgatgacgg agttcaaaaa agcctgcgtg cccggagcgc
    15001 agtccaccgg ctccgtctca taccagcccg cccgggcggt gagccgcgcg ctttccggca
    15061 gccgcgccag cagcagctcc a
//
LOCUS       DAOMYJ010000119        14979 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Prosthecobacter sp. isolate AMR_MDS_5629
            NODE_1354_length_14979_cov_21.917247, whole genome shotgun
            sequence.
ACCESSION   DAOMYJ010000119 DAOMYJ010000000
VERSION     DAOMYJ010000119.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867850
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prosthecobacter sp. (wastewater metagenome)
  ORGANISM  Prosthecobacter sp.
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Verrucomicrobiaceae; Prosthecobacter.
REFERENCE   1  (bases 1 to 14979)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14979)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.30
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:34:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,742
            CDSs (total)                      :: 4,684
            Genes (coding)                    :: 4,676
            CDSs (with protein)               :: 4,676
            Genes (RNA)                       :: 58
            tRNAs                             :: 55
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14979
                     /organism="Prosthecobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1354_length_14979_cov_21.917247"
                     /isolate="AMR_MDS_5629"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1965333"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..364)
                     /locus_tag="PLP58_13010"
     CDS             complement(<1..364)
                     /locus_tag="PLP58_13010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ09489.1"
                     /translation="MSIPPSLIQLTPPSTNTTGWQPVIGQETLDLLGHFAPKLDESER
                     DALQDSTVRILSQCAMPGAAAEVKTGLVVGYVQSGKTMSFTTVAALAADNNCPLIIVI
                     TGTTKPLFDQSSERLDRDL"
     gene            complement(361..1905)
                     /locus_tag="PLP58_13015"
     CDS             complement(361..1905)
                     /locus_tag="PLP58_13015"
                     /inference="COORDINATES: protein motif:HMM:NF024977.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HRJ09490.1"
                     /translation="MPSDTVNIQPGVNVLSVLRHLNYKPWYALGEFVDNAVQSALERW
                     EQLQELHGGKYRLKVEIELDPNDGGRITVRDNAAGIAVADYQRAFRTAEVPPNRKGLS
                     EFGMGMKSAGFWFSSNWSVRTKAVGEEHTGRIEFDLGKITAENLEELPVSFSAGQPHE
                     HFTELVLSHPQKMPAGRTLAKIKSHLTGIYRQFIRDGRLELWFRGDLLTFPEPPILNA
                     PHPKTPNAKPVVWRKEIDLTLTGGKRAHGFVAIREEASVAEAGLALFRRNRLIVGSGD
                     EGYRPESIFGQANDYRYQRVFGELHLEGFAVSHTKDGFQWEELEDEFQLKLKNMIDSE
                     PLPILAKAKEHRVRPKTSDIRRGAETAVSSTAAVLQSKAASVIQEQRDQPPENVVTPV
                     SLPPAAETAASKQFILDFKDQQWDVTIELANDPAIGEWLGVSDQPRAGQTRILKLRLN
                     CGHPFMERFAGSEAEQIEPLLRIAAAMAIAEIVARDQGYQHTSAVRRHVNELLTSALS
                     STTAIP"
     gene            complement(1886..3244)
                     /gene="dcm"
                     /locus_tag="PLP58_13020"
     CDS             complement(1886..3244)
                     /gene="dcm"
                     /locus_tag="PLP58_13020"
                     /EC_number="2.1.1.37"
                     /inference="COORDINATES: protein motif:HMM:NF012372.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003886 - DNA
                     (cytosine-5-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA (cytosine-5-)-methyltransferase"
                     /protein_id="HRJ09491.1"
                     /translation="MKFIDLFAGLGGFHVGLTNLGHECVFACELDEGLQDVYEKNFGI
                     RPAGDLRKVKNTDIPPHDILCAGFPCQPFSKAGAQDGFGCSRNGTLFDEVARIVEHRR
                     PEFVILENVPNLKKHNGGKTWQLIYERLQELGYATEANLYSPHEFGIPQIRQRILIVA
                     RRKSLGDFSWPERPKDKKPQLTSILDKKPDDAKPLSSQVTKCLEVWQEFLDLIPADEH
                     LPSWPIWSMEFGATYPFEDTTPHAVGADGLREYRGCHGVKLSSKTLEDPFDGLPSHGR
                     TPQDLFPSWKRYFIRANRAFYQKHKARFVNWLPKILEFPPSLQKFEWNCQGEPRDVWK
                     LVIQFRASGVRVKRPDTSPSLVAMTTTQVPIIGWERRYMTARECSRLQSLDSLEHLPE
                     TETRAFKALGNAVNAKLVEFIAGRLIPTEDKPLPTKVSAKVLHSSSSLHKTRPAHAIR
                     HR"
     gene            complement(3306..3467)
                     /locus_tag="PLP58_13025"
     CDS             complement(3306..3467)
                     /locus_tag="PLP58_13025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ09492.1"
                     /translation="MKRCLLFLLLAASAMAADTQNWPQFRGAAHDPIDGLHDLLLALL
                     NTHEFTVNH"
     gene            complement(3508..5451)
                     /locus_tag="PLP58_13030"
     CDS             complement(3508..5451)
                     /locus_tag="PLP58_13030"
                     /inference="COORDINATES: protein motif:HMM:NF019607.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-dependent sugar dehydrogenase"
                     /protein_id="HRJ09493.1"
                     /translation="MPPPLPALAYAMEEALGGLSFSMPVAVVSAPGEKDRLFVVEKTG
                     RIQEVTRLDEPMPEKREFANLIERPDGRLDDKGECGLLGLAFHPDFARNGRYFVYYSL
                     RIGGQLHQRVSCFEGGQEQPLITQLDPAGNHNGGDLHFGPDGYLYISVGDGGGANDAF
                     DNARFINKGFHAAILRIDVDKKPGSLPPNPHPAVHAGTYTVPADNPFIGATTHHGEKI
                     DPASVRTEIWATGLRNVWRFSFDPPTGRLFAADVGQNLYEEVNLIEKGGDYGWSHREG
                     LHAFDLGPGKDAVPPGFAPKVPIYEYPRTTGLSITGGCVYHGTKFPEFKDAYICADYA
                     FGRVIVLREKNGRWEDEIIAFEPAITGISADPRDGELLFCNMAKGKIMWLAEIEPELE
                     GVLFHENFEDTSVDARGWYDGKSVHIVPGGMRGNCIEYEWTDGSDSVTGSSAKRHLFE
                     ATDEVFIRYYLKLSKGWGWSGRSYHPHLTHFMTTENSKWHGPAASHLTLYIEPVNGKL
                     RLAAQDIQNKDAPHGLTQGPLKGGYNGQMFDSADELFTDDSWHCIEAQFKLNTLDLQN
                     DKPNADGIVRGWFDGKLVIDRTDVILRSTDFPNMKFNQFLMAPYFGPGLLPHAQKLWI
                     DEMAVGTKRIGPLPRERRPAKAP"
     gene            complement(5564..6271)
                     /locus_tag="PLP58_13035"
     CDS             complement(5564..6271)
                     /locus_tag="PLP58_13035"
                     /inference="COORDINATES: protein motif:HMM:NF020296.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
                     /protein_id="HRJ09494.1"
                     /translation="MTLEETMSILAAKGNAATKRTYMRHGAPEPLFGVRIGDLKPVQK
                     LIKGDQNLAMQLYATGNSDAMYLAGLVANGGRMPRALLNRWAKQATWHLISGCTVPWV
                     ASEHPQGFEIAMKWIEASEERVAVSGWSTLAAILAVTPDDELPIREIASLLTRVAKTI
                     RSSPNRVRYAMNNFVICCGTYVAPLAGKALAVAGEIGPVDVDMGNTDCQVPDAVSSIH
                     KSRRGLPVAPKRKTTRC"
     gene            6367..7692
                     /locus_tag="PLP58_13040"
     CDS             6367..7692
                     /locus_tag="PLP58_13040"
                     /inference="COORDINATES: protein motif:HMM:NF012428.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HRJ09495.1"
                     /translation="MFQRSQELFERAQRSIAGGINSGIRKMEAPVPLYFDHGKGCRLW
                     DVDGNEYMDFQTGQGALLYGHAPEGMAEAISAQARKGTHWAAQCELEIEVAERLQKMI
                     PGAERVRFNNSATEVVLSAFRLARAHTGRPLILRFEGHYHGWGDEGLVGFAPPPDRWG
                     DEENPARLHPSQGVIPEVLEQFVVARWNDIEHLRRRVDEHRGRIAAIVFEPVLCNTGC
                     MEPVPGLLQTIRELCDRDGMMMIADETITGFRFGAGGAQTHYGVVPDLTILGKAIGGG
                     VPFAAMAGKEASFAKILSGEVVHAGTLNANPLCLSAARWCLDQILALGDSHPAGIMAL
                     GKKMMSGLRALADQHGIPLRPQGPGLVFHASMLKPGAGEGAITSYRDYVKRHDAPRWA
                     HLRRCLLESGVRAIERGLWFLSLAHTEADVEEALRRTDAAFARHAAEWK"
     gene            complement(7700..9643)
                     /locus_tag="PLP58_13045"
     CDS             complement(7700..9643)
                     /locus_tag="PLP58_13045"
                     /inference="COORDINATES: protein motif:HMM:NF024627.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HRJ09496.1"
                     /translation="MRRFALSLAASSCLGFSQAAQYHVSPDGLDTHDGSSSHPWRTLT
                     HAAAQLQPGDTLLVRGGSYAERLVLNGKNGTESQPVTIRNAEGHAPVVDGQTLTVPSG
                     GIQGLVAFLNCSHLRFEGIEVANFVTSAAARIPAGIHIEGAGSGLQIRGCKVRRIWQS
                     STNGSANGFGIAVYGTSSAPLDGLVIDGCEVHDLRTGQSESVVLNGNVTNFAVINNTV
                     HDCNNIGIDFIGYEGSAPEGFDRARNGVCAGNTVYNIDSAYNPGYGGGFTSGGGDRSA
                     AGIYVDGGRDIIIERNHCHHCNFGAELASEDASGFTEGIILRNNLFHHNMNAGLIMGG
                     YDRLRGRTRNCKILNNTFYRNDTDENWSGQVAIQFYFEDNIFQNNIVWASPVTKQMIV
                     HYVTGGTAAQRAFPAGNVFDNNLYFVEGAESEAEFGLNPTGSGTSQGNQSYYGLAAWR
                     TAVGGDAASAFHDPGFVTPAPSASPVAADFKITGASFARDKGDPGFVPAPGEKDFFGA
                     SRVAGGRVDAGMHEFMTPWQSWRDSHFQKPDGGPGAEALSDPDFDLVANLIEYSQGMN
                     PSQPDAHLAPFAMSQGGHLRFQYRKAAPGLTYQVRVSTDLPGTSPWPLASGPEMNDGA
                     GVYWRDFPLDGDRLFVKLEVSLP"
     gene            9724..10455
                     /locus_tag="PLP58_13050"
     CDS             9724..10455
                     /locus_tag="PLP58_13050"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HRJ09497.1"
                     /translation="MNLQDQVCVITGAARGIGLAAAKALVTRGARVVLADLDAAALAE
                     AVEALPEGRALAVQADVTHAEEMERVVVETRARWDEIAVWINNAGLARHRWITDYNEG
                     EIDLMLAVNLKGTILGSQAALRAMRPQRRGHILNIISTASLRGIPSETVYCAAKWGVR
                     GFTQGLAEEAAPHRIRVTAILPGGVDTAFWDDASERAAPKQLFLKPEHIADGITRCIE
                     MDDFCVPRELVLRSLDDSDFSVRPE"
     gene            complement(10461..11084)
                     /locus_tag="PLP58_13055"
     CDS             complement(10461..11084)
                     /locus_tag="PLP58_13055"
                     /inference="COORDINATES: protein motif:HMM:NF015510.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA];
                     GO_process: GO:0044874 - lipoprotein localization to outer
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein carrier protein LolA"
                     /protein_id="HRJ09498.1"
                     /translation="MRLVCLFLLALLPAAAQQPAPKPLPPVLLQWAEAQKNLGDMEVP
                     FVQTRTTPALKEPAVTPGRFWRFADGCFRWELGRPAATVLVHDSQEFRVRESADDEWR
                     ALDAEDPRWRMWSRFLSGRESSPEELRRHFLVDAEQQTADVTAVSLRPKAPFLRKHLR
                     QLDLQISPQTSRLVQLRVLQGDGSTLLMTFDAPRPVNSADKALLLAR"
     gene            complement(11175..11804)
                     /gene="thiE"
                     /locus_tag="PLP58_13060"
     CDS             complement(11175..11804)
                     /gene="thiE"
                     /locus_tag="PLP58_13060"
                     /EC_number="2.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009964935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004789 - thiamine-phosphate
                     diphosphorylase activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HRJ09499.1"
                     /translation="MKDRILLARLYGIVDLGYTCPTQVVEMTRALCEGGVDILQLRAK
                     GLPPAEVERLARKMLPVTHDHDVPLIINDHIPVAATLGGVGVHVGQDDDSVARARAAL
                     GLDAVVGKSTHSLEQAAAARMEGADYIGFGPLYATGTKPDYTPIGLGGIAAVHGMVDV
                     PVFCIGGVNLPRLPEILAAGARRVVVVSAFLMAADVRSEVMRVKAMLPG"
     gene            complement(11840..13123)
                     /locus_tag="PLP58_13065"
     CDS             complement(11840..13123)
                     /locus_tag="PLP58_13065"
                     /inference="COORDINATES: protein motif:HMM:NF024753.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HRJ09500.1"
                     /translation="MKSTLLSSLLALTCALHAADWPRFLGPNGAAVVKDADVPLTWSD
                     SENIRWKFETPGPGSSSPIVSGGRVFLTCWSGYGDKEGAGDISKLKRHLLCLDLEKGV
                     KLWEAVIPSMAGEDPWQGFITEHGYATHTPVTDGAHVFVFFGKTGAMAFDMEGNQVWH
                     TPLGSGSGRNRWGSGGSPVLHGDMLIVNASDESRAFFALDKKTGKEIWKADGDAVGMA
                     YGTPSLIESGGRTDLVVALAEEVWGMNPETGKLRWFAAHRLPGNVSPTIVHDATTAYL
                     YGGYPTAGSVAVKLGGKGDVTQSHIVWSKSTSSYIPTPILHEGRLYVVNDAGFALCVD
                     AATGEDIYRERVIDSGGGRGRGKPFYASPVLVGDRLYCVSRRGGTYVIAARPEYQKLA
                     HNVLSLDDSQFHGTPAVLPDGLLLRSDKALYRIGK"
     gene            13243..13986
                     /locus_tag="PLP58_13070"
     CDS             13243..13986
                     /locus_tag="PLP58_13070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018968964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA pseudouridine synthase"
                     /protein_id="HRJ09501.1"
                     /translation="MLRAVNTAFTVLDEGADWLVVDKPAPLQIHPGTPDGTPTLWHGL
                     RDLLAYEIANDGQVGIINRLDRETSGVVLVAKTQAAARRFGIAMQERRIRKKYLAVVR
                     GWPEWSVLELDAPILRQGEVEHSAIWVRQRVHPEGAPCRTDFRLLKKVTHPAAGPLSL
                     VEAAPLTGRMHQIRAHLAHLGHPVLGDKIYGPDPQCYLDFIETGWTPELERRLLFARQ
                     ALHSHWLEVADFHPAWTAPMPPEMARWVE"
     gene            14048..14773
                     /locus_tag="PLP58_13075"
     CDS             14048..14773
                     /locus_tag="PLP58_13075"
                     /inference="COORDINATES: protein motif:HMM:NF018178.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1080 domain-containing protein"
                     /protein_id="HRJ09502.1"
                     /translation="MKMLLSLFLLLVSFTAQGAEGFMPIFNGKDLAGWVVPEGDNGHW
                     KVLDGVIDYDACSEAPGKDKNLWTAGEYKDFTLKIDWRLKETTGLYPMPVVLPDGTHK
                     LDENGREILIPTPNADSGIYLRGQGKSQINIWCWPVGSGEVYGYRMDKSMPPEVRAGV
                     TPKVKADKPVGEWNTFEITLKGDRLTVVLNGQTVIENAQLPGIPESGKIGLQHHGGRN
                     AKTGELSAASSLIQFRNISIREL"
BASE COUNT         2823 a         4524 c         4738 g         2894 t
ORIGIN      
        1 gcaggtcgcg atcaagcctc tcactcgact ggtcaaacaa cggctttgtc gtgcctgtga
       61 tgacgataat cagagggcag ttgttgtccg ctgctagtgc cgcaaccgtg gtgaatgaca
      121 tcgttttgcc gctctgcaca tatcccacaa cgaggcccgt cttcacctcg gcagccgccc
      181 ctggcatcgc acactgcgag aggatgcgga cggttgagtc ttgaagtgca tcacgctctg
      241 actcatcgag ttttggggca aaatgcccaa gcagatcaag cgtttcctgt ccgattactg
      301 gctgccaacc tgtggtattt gtcgaaggag gcgtgagttg gatgagagag ggcgggatgc
      361 tcatggaata gcggtggttg agcttaatgc agacgtgagt aattcgttca catgccgtcg
      421 gacggctgac gtatgctggt agccttgatc gcgagctaca atttcggcga ttgccatcgc
      481 agcagcgatt cttagtagag gttcgatttg ttctgcctca cttcctgcga aacgttccat
      541 gaacggatga ccgcaattca ggcggagctt gaggatgcgg gtttgtcctg cccgaggctg
      601 gtcactgact cccagccatt ctccgatagc aggatcattc gccaactcga tcgtcacatc
      661 ccattgctgg tctttgaaat ccaggatgaa ctgcttgctc gcggccgtct cagccgccgg
      721 cgggaggctc actggagtca cgacattctc aggtggttga tcgcgttgct cctggatgac
      781 actggctgct ttcgattgaa gaactgctgc tgtggatgac acggcggttt ctgcaccgcg
      841 tctaatgtcc gaggttttgg gccgcaccct gtgttccttg gcctttgcga gtatcggcag
      901 cggctcgctg tcgatcatgt tcttgagttt gagttggaat tcgtcctcaa gctcctccca
      961 ttggaacccg tctttggtat gactgacggc gaagccctcc aggtgcagtt cgccaaacac
     1021 acgttggtat ctgtagtcgt tggcttgtcc aaaaatgctt tcggggcgat acccttcgtc
     1081 cccactcccc acgataagcc ggtttcggcg aaagagcgcg agtcccgcct ctgcgacact
     1141 cgcctcctca cgaatcgcca cgaagccatg cgcccgtttc ccgccagtaa gggtgaggtc
     1201 gatctctttt ctccagacga ccggtttggc attaggggtc ttgggatgag gtgcgttcag
     1261 aatcggaggc tcggggaaag tgagcagatc accacgaaac cagagttcca aacggccatc
     1321 gcgaatgaac tgccggtaaa tgcctgtgag atgagacttg atcttcgcga gcgttcggcc
     1381 agcaggcatt ttctgcgggt gggagagtac aagctcggtg aagtgctcat gcggctgacc
     1441 agcgctgaaa ctcaccggca gttcttccaa attctcagca gtgattttgc cgaggtcgaa
     1501 ttcgatacgt ccagtgtgtt cttcaccaac agctttcgtt cgcacagacc agtttgaaga
     1561 aaaccagaac ccagcacttt tcatccccat gccgaactct gagagtcctt ttcgattcgg
     1621 cggcacttcg gctgtgcgaa aggcgcgctg atagtcagca accgcgattc cggctgcatt
     1681 gtccctcaca gtaattctac caccgtcatt cggatcgagt tcaatctcga ctttgagccg
     1741 atacttgcca ccatgcagtt cttgaagctg ttcccagcgc tcaagcgcac tctgaactgc
     1801 attgtccacg aattccccca gcgcatacca aggtttgtag tttaagtgcc gaagaactga
     1861 gaggacattt actcccggct ggatgttaac ggtgtcggat ggcatgtgca gggcgagttt
     1921 tgtgaagtga tgaagacgag tggagaactt tcgccgacac cttcgtcggc aaaggtttgt
     1981 cttccgttgg gatcagtcgt cctgcgatga actcgactag tttggcattc actgcgttac
     2041 ctagggcttt gaaggctctt gtttccgtct cgggcaagtg ctctagagaa tcgagggatt
     2101 gaagacgcga gcactcgcgg gccgtcatgt aacggcgctc ccatccgatg atgggcactt
     2161 gcgtcgttgt catggcaacc agtgaggggc tggtatcggg gcgcttcacg cgcacccccg
     2221 acgcacggaa ctggatcacc agtttccaca cgtcacgagg ctcgccctga cagttccact
     2281 caaacttttg gagggaggga gggaactcaa gaatcttggg cagccaattc acaaaccttg
     2341 ccttgtgctt ctggtagaac gcccggttcg cccgaatgaa ataccgcttc cacgaaggaa
     2401 acaaatcttg aggtgtgcgt ccgtgggagg gaaggccgtc gaacgggtct tctaacgttt
     2461 tgctactgag cttcactcca tggcacccgc gatattccct caaaccatcg gctccgacag
     2521 catgtggagt cgtgtcctca aaaggataag tagcaccgaa ctccatcgac caaatgggcc
     2581 atgacggcag gtgttcatct gccggtatga ggtccaagaa ctcctgccag acctccaggc
     2641 atttcgtgac ttgagaactg agcggcttgg catcatcagg cttcttgtcg agtattgaag
     2701 tcaattgagg cttcttgtct ttcggacgct cgggccacga gaaatctcct agcgacttcc
     2761 ggcgtgccac aatcaaaatg cgctggcgaa tctgaggaat gccgaactca tgcggcgaat
     2821 acaagttggc ctctgtcgcg tagccaagct cttgaaggcg ctcatagatg agctgccatg
     2881 tcttgcctcc gttgtgtttt ttgaggttgg gcacattctc caagatcaca aactcagggc
     2941 ggcggtgttc gacaatgcgc gccacctcat caaagagcgt tccgttccgc gagcagccaa
     3001 agccgtcctg agcacctgcc ttggagaacg gctggcacgg gaatcccgcg caaaggatgt
     3061 cgtgaggcgg tatgtctgtg tttttgacct tcctcaaatc acccgcaggc cgaatgccga
     3121 agttcttctc gtaaacgtct tgaaggcctt catccagttc acacgcgaac acgcattcgt
     3181 gccccaagtt ggtcagccca acatggaatc cccccagccc ggcgaataga tcaatgaatt
     3241 tcattcaacc ttaatggcat ttagccgctc gcgtcgaaaa aggcaattgg cgttgcggca
     3301 aaaaatcaat gattcacggt gaactcgtgc gtgttcagca aggccaggag cagatcgtgc
     3361 aggccgtcga tcggatcatg tgccgcgccg cgaaactgcg gccagttctg cgtgtcagcg
     3421 gccatcgctg atgctgcgag cagaagaaaa aggaggcagc gtttcatagt ggtgatgagt
     3481 tggagtgaaa caagccgacg gctgccatca cggagccttg gccggtcttc tttcgcgtgg
     3541 tagcggaccg atccgcttcg ttccgacggc catctcatcg atccatagct tctgcgcatg
     3601 cggcagcagg ccggggccga agtagggggc catgaggaac tggttgaact tcatgttcgg
     3661 aaaatcggtg gagcgcagga tgacgtccgt gcggtcgatg accagtttgc catcaaacca
     3721 gccgcgcacg atgccgtcgg cattgggctt gtcgttttgg agatcgagcg tgttcagctt
     3781 gaactgggcc tcgatgcaat gccacgagtc gtcggtgaac agttcgtccg cgctgtcgaa
     3841 catctgaccg ttgtagccgc ctttgagcgg tccttgcgtc aaaccatgcg gcgcgtcctt
     3901 gttctgaatg tcctgcgctg cgagccgcag tttgccgttc accggctcga tgtagagtgt
     3961 gagatggctg gctgctggtc catgccattt cgagttctcc gtcgtcatga agtgcgtgag
     4021 atgcggatgg tagctccgcc cgctccagcc ccagcctttc gagagtttga ggtagtagcg
     4081 gatgaaaacc tcgtccgtgg cctcgaacag atgccgtttg gcggaagagc ccgtcacgct
     4141 gtcgctgcca tcggtccatt catactcgat acagttccca cgcatgccac caggcacgat
     4201 gtggacgctt tttccatcat accagccgcg tgcatcgacc gacgtgtctt cgaagttctc
     4261 atggaacaac acgccttcta gctccggctc gatctccgcc agccacatga tctttccctt
     4321 cgccatgttg cagaagagca gctctccgtc gcgtggatcg gcgctgatgc cggtgatggc
     4381 cggctcaaaa gcgatgatct catcctccca gcggccgttt ttctcgcgca acacgatcac
     4441 acggccaaac gcgtagtccg cgcagatgta cgcgtctttg aactccggaa acttcgtgcc
     4501 gtgataaacg cagccgccgg tgatgctgag accggtggtg cgtggatact cgtaaatggg
     4561 caccttcggt gcgaaaccgg gcggaaccgc gtctttaccc ggtccgaggt cgaacgcgtg
     4621 caacccctcg cgatggctcc agccgtaatc gccgcctttt tcgatcagat tgacctcctc
     4681 gtaaagattc tggcccacat cggccgcgaa gagccttcct gtcggcggat cgaagctgaa
     4741 gcgccacacg ttccgcagtc ccgtggccca gatttccgtg cgcacgctcg cagggtcgat
     4801 cttttcgccg tgatgcgtcg tcgcgccgat gaagggattg tccgcgggca ccgtgtaggt
     4861 gccggcatgc accgcgggat gcggattcgg cggcaggctg ccgggtttct tgtccacgtc
     4921 gatgcgcagg atcgcggcgt ggaagccttt gttgatgaag cgtgcgttgt cgaaggcgtc
     4981 gttcgctccg ccgccatcgc ccacgctgat gtagagataa ccatcaggcc cgaaatgcag
     5041 atcgccgccg ttgtgattcc ccgctggatc gagttgtgtg atcagcggct gctcctggcc
     5101 accctcaaag cacgatacgc gttgatgaag ctgcccgccg atgcgcaggc tgtaatagac
     5161 gaagtagcgg ccattgcgcg cgaagtcggg gtgaaatgcg agacccagca gcccgcactc
     5221 gcccttgtca tcgagcctgc catcgggtcg ttcgatgagg ttggcgaact cacgtttctc
     5281 aggcatcggc tcatcgaggc gcgtcacctc ctggatgcgg ccggtctttt ccacgacaaa
     5341 cagccggtcc ttctcgcccg gagcgctcac cacggccacc ggcattgaaa agctcagccc
     5401 gcccagcgcc tcctccatcg catacgccaa agccgggagc gggggtggca cgggcggcgg
     5461 ctttttgacc ttgggaattt gtgtctgcgc gtctgcagac accgcacaca acagagacag
     5521 aattgcggga gtgagtttca tgtcattttg tttcacccgc agtttaacac cgggttgttt
     5581 tacgcttcgg agccacaggg agaccgcgac ggcttttgtg gatgctggaa accgcgtcgg
     5641 gaacctggca gtcggtattc cccatgtcca cgtcgacggg accgatttca cccgcgacag
     5701 ccagggcttt tccagcaagt ggagccacat aggttccgca gcagatgaca aaattgttca
     5761 ttgcgtagcg aacccggttg ggggacgagc ggattgtctt cgcaacccga gtgagcaggg
     5821 atgcgatttc tcgaatgggc agttcgtcat ctggagttac cgcgaggatg gcggccaggg
     5881 tggaccagcc ggaaacagcg acacgctcct cggaggcttc aatccacttc atggcaattt
     5941 caaacccctg aggatgctca gaggcaaccc acgggactgt gcagcctgaa atcagatgcc
     6001 atgtggcctg ctttgcccag cgattcagca gggcgcgggg catcctgccg ccattggcaa
     6061 caagacccgc aagatacatc gcatcagaat tgcctgtggc gtagagttgc atggcgagat
     6121 tctggtcgcc tttgatcagt ttttggaccg gcttcaaatc accgattcga acgccaaaaa
     6181 gaggttccgg agctccgtgg cgcatgtatg tgcgctttgt tgcggcgttg cctttggcag
     6241 ccagaatgct catggtttct tcaagtgtca tatgggtttc cttttcgtgc ggtcgctgaa
     6301 ttttgaaggc gtttgaggat acgatgtgga aagaacgcgc ccagttgcct gcttatttgt
     6361 ctcctcatgt tccaacgctc ccaagaactc ttcgaacgcg cgcagcgcag catcgccggc
     6421 ggcatcaaca gcggcatccg caaaatggag gcgccggtgc ctctgtattt cgaccacggc
     6481 aaaggctgcc ggctctggga tgtggacggc aatgaataca tggacttcca gaccggccag
     6541 ggcgcgctgc tatacggcca tgcgccggag ggcatggcgg aggcgatctc cgcgcaggcg
     6601 cgcaagggca cgcattgggc ggcgcagtgc gagctggaga tcgaagtggc ggagcggctg
     6661 caaaagatga tccccggcgc ggaacgggtg cgcttcaaca actcggccac cgaggtagta
     6721 ctctccgcct tccggttggc ccgcgcccac acggggcggc cgctgatttt gagattcgaa
     6781 ggccattacc acggctgggg agatgaaggc ctcgtcggct tcgcgccacc gccggaccgg
     6841 tggggggatg aggagaatcc ggcgcggctg catccgagcc agggtgtgat ccctgaggtg
     6901 ctggagcagt tcgtggtggc taggtggaac gacatcgagc acctgcgccg ccgggtggac
     6961 gagcatcgcg ggcgcatcgc ggccattgtc tttgaaccgg tgctgtgcaa cacgggctgc
     7021 atggagccgg tgccggggct gctgcaaacc atccgcgaac tgtgcgaccg cgacggcatg
     7081 atgatgatcg ccgacgagac gatcaccggc ttccgcttcg gcgcgggcgg cgcgcagacg
     7141 cattacggcg tggtgccgga cctcaccatc ctcggcaagg ccatcggcgg cggcgtgcct
     7201 tttgcggcga tggcgggaaa agaggcgtct tttgccaaaa tcctcagcgg cgaggtggtc
     7261 cacgcgggca ccttgaacgc gaacccgcta tgcctcagcg cggccaggtg gtgcctggat
     7321 caaatcctcg cgctgggtga tagccacccc gccgggatca tggcgctcgg aaaaaaaatg
     7381 atgtccggcc tgcgcgcgct ggcggaccag cacggcatcc cattgcgccc gcaagggccc
     7441 ggcctcgtct tccatgcctc catgctgaag ccgggagccg gggaaggcgc catcaccagc
     7501 tatcgcgatt atgtgaagcg gcacgacgcg ccgcgttggg cgcacctgcg gcgctgcctg
     7561 ctggagagcg gcgtgcgcgc catcgagcgc ggtctctggt tcctcagcct ggcgcacacg
     7621 gaggcggatg tggaggaggc gctgcggcga accgacgcgg ccttcgcgcg ccatgcggcg
     7681 gagtggaaat aagacgcgct caaggcaggg acacctccag cttcacaaac aggcgatccc
     7741 cgtccagagg gaagtcccgc cagtaaacac ccgcgccatc gttcatctcc ggtccagatg
     7801 ccaggggcca cggacttgtt ccaggcaagt ccgtggagac gcgcacctga tacgtcaatc
     7861 ccggcgcggc tttgcgatat tgaaagcgca gatgcccgcc ctggctcatg gcaaaggggg
     7921 ccagatgcgc gtccggctgg ctggggttca tgccctgcga atactcgatg agattggcca
     7981 cgaggtcgaa gtccggatca ctcagcgcct ccgcgcccgg gccaccgtcc ggcttttgaa
     8041 aatgcgagtc gcgccaggac tgccagggcg tcatgaattc gtgcatgcca gcgtccacgc
     8101 gtccgcccgc cacccgcgag gcgccaaaga agtccttttc cccaggcgcc gggacaaatc
     8161 caggatcacc cttgtccctg gcaaaagacg ccccggtgat tttgaagtcc gccgccactg
     8221 gactggcgga cggcgcgggt gtgacgaagc ccggatcgtg aaaggcgctg gccgcatcac
     8281 ctccgacagc cgtcctccag gcggcgaggc cgtaataaga ctggttgccc tggctggtcc
     8341 cggagcctgt cggattgagg ccaaactcag cctccgactc ggcaccttcg acaaagtaga
     8401 gattattatc gaagacattg cccgccggga aggctcgctg ggccgccgtg ccgccggtga
     8461 cataatgcac gatcatctgc ttcgtcaccg ggctggccca gacgatgttg ttttgaaaga
     8521 tgttgtcttc gaaataaaac tggatggcca cctggccgga ccagttttca tccgtgtcgt
     8581 tgcggtaaaa agtgttgttc aagattttgc agttccgggt gcgcccgcgc aggcggtcgt
     8641 agccgcccat gatgaggccc gcgttcatgt tgtgatggaa gaggttgttg cgcaggatga
     8701 tgccctcggt gaagccggag gcgtcctcgc tggccagctc cgcgccgaag ttgcagtggt
     8761 ggcagtggtt gcgctcaatg atgatgtccc tgccgccgtc cacatagatg cccgccgccg
     8821 agcggtcgcc gccgccgctg gtgaaaccgc cgccgtagcc gggattatag gcggagtcta
     8881 tgttgtaaac ggtgttcccc gcgcagacgc cgttccgggc gcggtcaaac ccctcaggcg
     8941 cgctgccctc gtagccgatg aagtcaatgc cgatgttgtt gcagtcatgc acggtgttgt
     9001 tgatgacggc gaagtttgtg acattgccgt tgagcaccac ggactcgctc tgccccgtgc
     9061 gcaggtcgtg gacctcgcag ccatcgatca ccagaccgtc aagcggcgcg gacgaggtgc
     9121 catagacggc gatgccgaag ccgttggcgg aaccattcgt gctgctctgc cagatgcggc
     9181 gcactttgca tccgcggatt tgcagtcccg aacccgctcc ctcgatgtga atgccggccg
     9241 ggatgcgcgc ggcggcggag gtgacgaagt tcgccacctc gatgccctcg aagcgcaggt
     9301 gggagcagtt taaaaaagcc acgagtccct ggatgccgcc cgatggcacg gtcaaagtct
     9361 ggccgtcaac caccggcgcg tgcccttccg cgttgcggat ggtgacaggc tgcgactcgg
     9421 tgccgttttt gccgttcagc acgagacgct ccgcatagga gccgccgcgc acgagcagcg
     9481 tgtcacccgg ctggagctgc gccgccgcgt gggtgagcgt gcgccagggg tgtgaggagg
     9541 atccgtcatg ggtgtcgagt ccgtccgggc tgacatgata ctgcgcagcc tgggaaaaac
     9601 cgagacatga agaagcggca agtgacaagg cgaagcgacg catgcgccgg agtctaggcc
     9661 gcgcggcgca tccaggcaac cccgccgctt gtcattcatc cggcacgcgc catcatcgcg
     9721 cgcatgaatc ttcaagacca ggtctgtgtc atcaccggag ccgcgcgcgg catcggcctc
     9781 gcggcagcca aagcgctcgt cacacgcggc gcgcgagtcg tgctcgcgga cctcgatgcc
     9841 gccgcgctgg ctgaagccgt ggaggccttg ccggaaggcc gggcgctggc cgtgcaggcg
     9901 gatgtgacac acgcggagga aatggagcgc gtggtggtcg agacgcgcgc gcgctgggac
     9961 gagattgccg tctggatcaa caacgccggc ctcgcgcgcc accgctggat caccgactac
    10021 aacgagggcg agatagacct catgctggcg gtgaatttga agggaaccat cctcggctcc
    10081 caggccgcgc tgcgcgccat gcggccgcaa aggcgcggcc acatcctcaa catcatctcc
    10141 accgccagcc tgcgcggcat cccgagcgag acggtgtatt gcgcggcgaa gtggggcgtg
    10201 cgcggcttca cccagggcct ggccgaggag gccgcgccgc accgcatccg cgtcaccgcc
    10261 atcctgcccg gcggcgtgga caccgccttc tgggacgacg cctccgagcg cgccgctccg
    10321 aaacagcttt tcctcaagcc cgagcacatc gccgacggca tcacgcgctg catcgagatg
    10381 gacgacttct gcgtgccgcg cgagctggtg ctgcgctcgc tggatgactc ggacttctcc
    10441 gtgcggccgg agtgaggcgc tcagcgcgcc agcagcaggg ctttgtcggc gctgttgaca
    10501 gggcgcggcg cgtcgaaggt catgagcagg gtggagccgt ctccttgcaa cacgcggagc
    10561 tgcaccaggc gcgaggtctg cggggagatt tgcagatcga gctggcgcag gtgcttgcgg
    10621 agaaagggag ccttgggacg caggctgaca gcggtgacat ctgcggtctg ctgctcggca
    10681 tccacgagga agtggcgccg cagttcctcc gggctggact ccctcccgct gagaaaacgc
    10741 gaccacatgc gccagcgcgg atcctcggcg tcaagcgcgc gccattcgtc atcagcgctc
    10801 tcccgcacgc ggaactcctg cgaatcatga acgagcacgg tggcggcggg ccgccccaac
    10861 tcccagcgaa agcagccgtc ggcaaaacgc cagaatcggc ccggtgtcac ggccggttct
    10921 ttgagcgcgg gcgtggtgcg cgtctggacg aagggcacct ccatgtcgcc caggtttttt
    10981 tgcgcctccg cccactggag gagcacgggt ggcagcggct ttggcgcggg ctgctgggcg
    11041 gccgcaggca ggagtgcgag caggaaaagg caaacgaggc gcatgggcag tggggggagg
    11101 ctcgaaattc gaaaaaacag tgatcgaagc tcaagcatcc atcttcggat ttcgagcttc
    11161 gaatttcgat tttttcagcc tggcagcatg gctttcacgc gcatgacctc ggagcgcaca
    11221 tcggcggcca tgaggaaggc ggagacgacg acgacgcggc gcgcgccggc ggccaggatt
    11281 tcggggagcc ggggcagatt gacgccgccg atgcagaaga cgggcacatc caccatcccg
    11341 tgaacagcgg cgatgccacc caggccgatg ggtgtgtagt caggcttggt gccggtggcg
    11401 taaagcgggc cgaagccgat gtaatccgcg ccctccatcc gcgcggcggc ggcctgctcc
    11461 aggctgtggg tggacttgcc caccacggcg tcaagcccca gggcggcgcg cgcgcgggcc
    11521 accgagtcgt catcctggcc gacatgcacg cccacaccgc cgagggtggc ggccacgggg
    11581 atgtggtcgt tgatgatgag cggcacatcg tggtcatgcg tgacgggcag catctttctg
    11641 gccaggcgct cgacttcggc gggcggcagc cccttggcgc gcaactgaag gatgtccacg
    11701 ccgccctcgc acagggcgcg ggtcatttcc accacctggg tggggcaggt gtagccgagg
    11761 tccacgatgc cgtagagccg ggccaggagt atgcggtcct tcatgcccga gttttagccg
    11821 ccgaaagcgg gaaggcaact cacttgccga tgcggtagag ggctttgtcg ctgcgcagca
    11881 gcaggccgtc aggcagcacg gccggagtgc cgtggaactg ggagtcgtcg aggctcagga
    11941 cgttgtgggc cagtttttga tactccggcc tggcggcgat gacgtaggtg ccgccgcgtc
    12001 ttgaaacaca gtagagccgg tctccgacga gcactggtga ggcataaaat ggcttgccgc
    12061 gcccgcgccc gccgccgctg tctatgacgc gctcgcggta gatgtcctcc ccggtggcgg
    12121 cgtccacgca gagggcgaac ccggcgtcgt tgacaacgta aagccggcct tcgtgcagga
    12181 tgggggtggg gatgtaactg ctggtgcttt ttgaccagac gatgtggctc tgcgtcacat
    12241 cccccttgcc gccgagtttg acggcgacac tgccggcggt ggggtagccg ccgtagagat
    12301 aggcggtggt ggcatcgtga acgatggtgg gggagacatt ccccggcagg cggtgggcgg
    12361 cgaaccagcg tagtttgcca gtctccgggt tcatgcccca cacttcctcg gccagggcga
    12421 cgacgaggtc ggtgcgcccg ccggattcga tgagggaggg ggtgccatag gccatgccga
    12481 cagcatcacc atccgccttc cagatttctt tgcccgtttt tttgtcgagc gcgaaaaacg
    12541 cgcggctttc atcgctggcg ttgacgatga gcatgtcccc gtgcaggacg gggctgccgc
    12601 cggagcccca gcggttgcgg cccgatccgc tgccgagcgg ggtgtgccag acctggttgc
    12661 cctccatgtc gaaggccatg gcgccggttt tgccgaaaaa aacgaagaca tgcgcgccat
    12721 cggtgaccgg tgtgtgcgtg gcgtagccat gctcggtgat gaagccctgc caggggtcct
    12781 ccccggccat ggaggggatg acggcctccc agagtttcac gcccttttcc aggtcgaggc
    12841 agagaagatg gcgcttgagc ttggagatgt cgccggctcc ctccttgtcc ccgtagccag
    12901 accagcaggt gaggaagacg cgcccgccgc tgacgatggg gctggaggag ccgggaccgg
    12961 gcgtttcgaa tttccaacgg atgttttccg agtcggacca ggtgagaggc acatcggcgt
    13021 ccttgacgac agcggcgcca ttcgggccga gaaagcgcgg ccagtcagcc gcatgaagcg
    13081 cgcacgtcag cgcgagcagg gaggaaagca gggtggattt catcgcgggc gtgttcaaca
    13141 cagcggacgc agcgaggttg cgaaagcaag tcaggcgtgg cagtcaggcg tggcatttcg
    13201 actttaaaaa ttcgaaactc gaaacttcca tcttccctcc ctgtgctccg cgccgtcaac
    13261 accgcgttca ccgtcctcga cgagggcgcg gactggcttg tggtggacaa gcccgcgccg
    13321 ctgcagatcc accccggcac gcccgatggc acgcccacgc tctggcacgg gctgagggat
    13381 ctgctggcct acgagatcgc caacgacgga caggtgggca tcatcaaccg gctggaccgc
    13441 gagaccagcg gcgtggttct ggtggccaag acgcaggcag ccgcgcggcg cttcggcatc
    13501 gccatgcagg agcggcgcat ccgcaaaaaa tatctggccg tggtgcgcgg ctggccggag
    13561 tggagcgtcc tggagctgga cgcgcccatc ctgcgccagg gcgaggtgga gcactccgcc
    13621 atctgggtgc ggcagcgcgt gcatccggag ggcgcgccct gccgcacgga cttccgcctt
    13681 ttgaaaaaag tgacgcatcc cgcggcaggc ccgctctccc tggtggaagc cgcgccgctc
    13741 acgggccgga tgcatcaaat ccgcgcgcat ctggcccacc tcggccatcc cgtgcttggc
    13801 gacaaaatct acggacccga cccgcagtgt tacctggatt tcatcgagac tggctggacg
    13861 ccggagttgg agcggcggct gcttttcgcc cgccaggcgc tgcactcgca ctggctggaa
    13921 gtggcggact tccaccccgc ctggaccgcg cccatgcctc cggaaatggc gcgctgggtg
    13981 gagtgaggcg tgtctgccaa gattcgacgc aagccagccc gtgccagcca acgttatggc
    14041 aactgccatg aagatgctcc tctccctgtt cctcctgctt gtctctttca ccgcgcaagg
    14101 cgccgaaggt tttatgccga tcttcaacgg caaagacctc gccggctggg tcgtccccga
    14161 gggagacaac ggccattgga aggtgctcga cggcgtcatt gactacgacg cgtgcagcga
    14221 ggcgcccggc aaggacaaaa acctgtggac cgcgggggag tacaaggact tcaccctgaa
    14281 aattgactgg cggctcaaag agaccaccgg gctctatccc atgcccgtcg tgctgccgga
    14341 cggcacgcac aaactcgatg aaaacggcag ggagatcctc atccccaccc ccaacgccga
    14401 cagcggcatc tacctgcgag gccagggcaa atcccagatc aacatctggt gctggccggt
    14461 cggctccggc gaggtctatg gctaccgcat ggacaaatcc atgccgccgg aggttcgtgc
    14521 gggagtgacc cccaaagtga aggccgacaa gcctgtgggc gagtggaaca ccttcgagat
    14581 caccctgaag ggcgaccgcc tcaccgttgt gctcaacggc cagaccgtga tcgagaacgc
    14641 gcaactgccc ggcattcctg aaagcggaaa aatcggcctc cagcaccacg gcgggcgcaa
    14701 cgccaaaacc ggcgagcttt ccgccgcctc cagcctcatc cagttccgca acatctccat
    14761 cagggagctt tgagcggcgg aacaggatgc tcctatgcgg acggcggctt gagtggccgc
    14821 cgggtgaatg aacgcggcgg ttcctcgtct cggtaatgtc ccgcctctca attccgcatg
    14881 aacgcagact ccgcgacatc caactcccct cgttccgcag gccgcaggct gctcgctcta
    14941 gcctgggcgc accgggcgcc ggcggcgggg gtgctggcc
//
LOCUS       DAOMYJ010000120        14973 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Prosthecobacter sp. isolate AMR_MDS_5629
            NODE_1357_length_14973_cov_22.759083, whole genome shotgun
            sequence.
ACCESSION   DAOMYJ010000120 DAOMYJ010000000
VERSION     DAOMYJ010000120.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867850
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prosthecobacter sp. (wastewater metagenome)
  ORGANISM  Prosthecobacter sp.
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Verrucomicrobiaceae; Prosthecobacter.
REFERENCE   1  (bases 1 to 14973)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14973)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.30
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:34:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,742
            CDSs (total)                      :: 4,684
            Genes (coding)                    :: 4,676
            CDSs (with protein)               :: 4,676
            Genes (RNA)                       :: 58
            tRNAs                             :: 55
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14973
                     /organism="Prosthecobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1357_length_14973_cov_22.759083"
                     /isolate="AMR_MDS_5629"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1965333"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..840
                     /locus_tag="PLP58_13080"
     CDS             <1..840
                     /locus_tag="PLP58_13080"
                     /inference="COORDINATES: protein motif:HMM:NF027890.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family efflux transporter periplasmic
                     adaptor subunit"
                     /protein_id="HRJ09503.1"
                     /translation="PGAVAARVPQLAKAKADVESAKAQLAKARRDLERTILRAPYAGQ
                     VLEQTADVGQMVGPGTTLGQIFAVDYVEVRLPLPERESQFLSLPQPFRDGAARSGGKA
                     LLKATVAGKPAQWEGRVVRVESALDETTRQVMAVVQVDDPYGRREDGTPPLTIGAFVE
                     AELAGEPLRDVFVIPRAAVRAGNEIILIDRPQNTLRRRVVEPLVSNSRHIVVDSASVS
                     APKEGDVLCLTPIPFPADGARVLPTIDGQVESPPVGKKAAKQPEDGEPKPKAPVVSQN
                     DGK"
     gene            898..1347
                     /locus_tag="PLP58_13085"
     CDS             898..1347
                     /locus_tag="PLP58_13085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ09504.1"
                     /translation="MKNTLFILLLTALLTSCSAGFRREWNTALKTGPQPGVAGAWEGT
                     WKSHVNGHHGRLRAVVSAPKNDTGDHDFHYHATWARILSGGFRAEHRVTPLRGGGSSF
                     KGSHQMPDWAGGLYTYEGTVKGDSFEACYECGKDKGVFSMRRAPQAR"
     gene            complement(1316..2122)
                     /locus_tag="PLP58_13090"
     CDS             complement(1316..2122)
                     /locus_tag="PLP58_13090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001296333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA threonylcarbamoyladenosine dehydratase"
                     /protein_id="HRJ09505.1"
                     /translation="MTESWFQRFGGIGRLYGAAALPHLHAAHVCVVGVGGVGSWVVEA
                     LARSGLGALTLVDMDDVCVTNTNRQLPALAGTVGWPKVRVLEERVRAINPECRVAAVA
                     EFLLPGSADRILAPGFDVIVDAVDDTGIKALIIAKCRALGIPVVVSGSAGGRRDPTQI
                     RAADLGLAGADPLLQQVRRLLRSEHGFAKSTDGRPMDWGVPCVYSTEKAVYPQADGTC
                     SPERDGGGQGLRLDCAAGFGAAAFVTGAFGFALAAEVLKIITAPAARASC"
     gene            complement(2183..2464)
                     /locus_tag="PLP58_13095"
     CDS             complement(2183..2464)
                     /locus_tag="PLP58_13095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ09506.1"
                     /translation="MRLILLLLIVVLAGLAFTNPNEADFREHVRQQEGLAGTLGLVVA
                     DLVSGGKKGGIQRDNYIFASKFYLGGDGILPREDIAWGIGGQFIEINRR"
     gene            complement(2522..3763)
                     /locus_tag="PLP58_13100"
     CDS             complement(2522..3763)
                     /locus_tag="PLP58_13100"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HRJ09507.1"
                     /translation="MPAPRPSFWKWYVCGLLLLATTINYMDRQTLSNAAVRVTREFGL
                     SQEQYGDLELAFGWAFAAGSLVFGFLADRFRVYWLYPIVLAGWSLMGIASAHTRDYPE
                     LMICRVLLGFFEAGHWPCALKTTFALLRPEDRTMGNSVLQSGASIGAVVTPPIMIWLM
                     TEDVSSWRLAFQVIGAAGLVWVVLWFASIRRVELDVVAPMEKAAFEGLLGILLGRRFW
                     ALAALIFGAQTCWHLFRVWLPKFMQEGRGYSETEALAFNSAYYIGTDIGCVAAGIISL
                     WLARRFGMTAHNARRLVYAGACVLTSFSLLVLWLPKGWALMAAILVVGAGALALFPCY
                     YSFVQELSDRHVGRLTGLLSTWVWAVSSPMHKLFGKLVDETKSFDLGMAMAGLAPWLG
                     VVAMWLLWDRSGVKTTPQAGG"
     gene            3886..4734
                     /locus_tag="PLP58_13105"
     CDS             3886..4734
                     /locus_tag="PLP58_13105"
                     /inference="COORDINATES: protein motif:HMM:NF016777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HRJ09508.1"
                     /translation="MIFDVHSHAWNFPADFSEDFITQAGRARPGVKVDLSATYEAYRA
                     AAPDDTHTIVFGGKARLSGLWVDDKTVAEYVGRDPARLTGFLSVDPTRPGWENEMRAG
                     HEELGLRGIKLLPMYAGFSPDDPALEPLWIYAEANGLPVLLHMGTTFIAQAPLAFTLP
                     RLIEPVAQRHPGVKIILAHLGHPYEGECIVTIRKHENVFTDVSALHYRPWQFYHSLML
                     AQEYGVWHKLLFGTDFPFTTVNASIGGIRGLNDMLEGTRLPRLDAEQIEAMIHRDSLA
                     LLGLRH"
     gene            4781..6394
                     /locus_tag="PLP58_13110"
     CDS             4781..6394
                     /locus_tag="PLP58_13110"
                     /inference="COORDINATES: protein motif:HMM:NF013884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HRJ09509.1"
                     /translation="METPRANSRRHDLDALRAFAMLLGIALHAALSFTGGPWMVQDTR
                     TSGLFHLMVGAVHGFRMPLFFLLSGFFTAMLWRKRGLRALLAHRFKRVFLPLVLGSVT
                     IVPVMFLLGGVSAKSGARQEESKPATLWHACAAGDEDLVRGFLGDGADVNAPDPLLGA
                     TPLTHASLFGRENVARLLLERGADANKTNRDGGTALHAAAFLGRAGIVRMLLGAGADP
                     RVRNERGDTALDAARTDLRATQFLARILRVNVEMDALQAGRAETRRLLGGDASEEKPR
                     PLLGVWLFLRHMPVFAHLWFLWFLCWMFAGFAMCARLADGIGWRPAPRARVTSAWRYL
                     WLVPLTLIPSWLMAQAGQNFGPETSSSILPPAHLLAYYAVFFGFGALWHDSMDTAGRL
                     GRCWWLTLPLALLLVYPAGLAATLGGAKLRGAAVLLQVLYAWLMCVAMIGLFRRFVSG
                     ERGWVRYLSDASYWMYLAHLPLVVAAQMWARDWPLPAGMKFLLVCAGVFALLLASYRW
                     LVRHTWIGALLNGRKERPPMSQPAVTGSP"
     gene            complement(6367..7725)
                     /gene="miaB"
                     /locus_tag="PLP58_13115"
     CDS             complement(6367..7725)
                     /gene="miaB"
                     /locus_tag="PLP58_13115"
                     /EC_number="2.8.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009958233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /protein_id="HRJ09510.1"
                     /translation="MPRVYIKTYGCQMNERDTEQVSQMFVERGYTLTPTDEDADVVLI
                     NTCSVRDQAEQKALGKMGMARRRRVPLVTGFMGCMAQSRGPELVEKSGVDLVVGTQKY
                     HRVVDYVDRIIRAKEERQMDDERYSIVDVGEEEGSQDTIRDHTLKERQGSAFVSIMQG
                     CNMKCTFCIVPYTRGGERARPIAGIVEEVRRLADRGVKEVTLLGQIVNLYGRHEFPVV
                     DGKSPFVQLLEAVHEVPGIQRIRFTSPHPIGYKKDLIEAFTRLPKLAEHVHLPVQSGS
                     DEILKKMHRPYTAGKFADLVERIRAARPGIAVTTDVIVGFPGETDAHYQETRALFERV
                     RFDNAFVFRYSKRRGTPAAEMDESVQVPERVKEERNQDLLALVNSHALPRYDELVGQN
                     VEILCEGPSKTTETRLMGRTRTNKIVVFEGSAERHTGEIFDVRVLSHHHFTLYGDPVT
                     AG"
     gene            7842..9134
                     /gene="lysA"
                     /locus_tag="PLP58_13120"
     CDS             7842..9134
                     /gene="lysA"
                     /locus_tag="PLP58_13120"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012419772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008836 - diaminopimelate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HRJ09511.1"
                     /translation="MHHFHYSQGGLFVEDVSLQSLAEKHGTPLYVYSKGTITSHFTRL
                     DAALSGLDHLICYAVKANSNLAVLATIARLGGGFDIVSGGELYRVIKAGGDPRKCTFA
                     GVGKTREEIAYALDQGIYCINAESEAELRHINQIAGELGKKAPVAVRVNPNVDAKTHA
                     KITTGKSENKFGVDFENILDVYARAAAECQHLELRGLQMHIGSQLTSVEPFVEAVRKV
                     VPLVQEVKARHGIQFFSIGGGIGINYRQSLDSGPESWWQENENLHPLTIQAYADAVTP
                     LLQPLGLRILCEPGRFMVGNAGALITRVLYEKRGAAKIFKIVDAGMNDLIRPTLYEGW
                     HQIVPLKQPATGEVEKADIVGPICETGDFLAQDRELPPVQAGDYLAVLSAGAYGFTMA
                     SNYNTRPMPAEILVDGAKASVVRERQTLDDVLKGERVE"
     gene            complement(9150..10199)
                     /locus_tag="PLP58_13125"
     CDS             complement(9150..10199)
                     /locus_tag="PLP58_13125"
                     /inference="COORDINATES: protein motif:HMM:NF014523.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MlaD family protein"
                     /protein_id="HRJ09512.1"
                     /translation="MNSERKTEVLVGLFLFVGMLMLGGIILQFGGLREWFTDSYRLRV
                     SFPNASGIKEGSPVYLGGSKVGKVLRHPQLNETFTGIIVDLKINEDVDIPADSAFAIG
                     SAGLMGDALIEIRPGSEGATAFIPHDHAEIIEGVKSGGLSDLQSTAEEVGKKVDLVLD
                     DLRAALKDIRESVSKINKDALSDATITDFKDSMEHLSQTMKRVDEKVLGDENAGMLKS
                     TLADLQTAAASFKTSSKNVEEGTAKIGLMLDKLEPAIAKVDKVVTSAGDTLQSIKSAA
                     DSLALTARNLNSGKGILAALMNDPALKTDLHDLIANLKRNGVLFYRDSAARERAKEEA
                     QPGPVRQGPRPLFGR"
     gene            complement(10209..10973)
                     /locus_tag="PLP58_13130"
     CDS             complement(10209..10973)
                     /locus_tag="PLP58_13130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009965212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRJ09513.1"
                     /translation="MPGTDSSVPFIRVTGLKKSFGAQKTLQGVDLDICHGETLVLIGP
                     SGEGKSVLLKHIIGLLHPDEGRVELDGVNLCSMTERQLLIARQRMGYLFQNAALFGSL
                     TVAENVAFPLKEAGLRDQKQIADRVHEALDLVELSAHKDKMPVNLSGGMRKRAGIARA
                     IVTRPECILYDEPTAGLDPVVTDVIDHMIQRMQKRFRVTSIVITHDMSSVFRIADRVV
                     MLKNGVVSFNGTPGELRASPEPEIQNFIAGRSGVIA"
     gene            complement(10977..11783)
                     /locus_tag="PLP58_13135"
     CDS             complement(10977..11783)
                     /locus_tag="PLP58_13135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009965211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRJ09514.1"
                     /translation="MSAFAEMLALPGRSFLNFLSYLGQLGRLVADLAHSIFRGRLRIL
                     QMAEQIVGIGYGSQAVVVVTGAFTGAVFTAQSYFKFKDFGIETTIGGIVSVSLCRELA
                     PVLAGLMVTGRVGAAMAAEIGTMKVNEEIDALRVMGVHPVDYLVQPRFFAMMISMPLL
                     IAESIVFGLAASALVSSLAFHIPFTWFWTHVVDHTNHTDLIFGMTKGFFFGILIVLIS
                     CHQGLTAANGAVGVGLGTTRAVVFSSLALLVVNFFLTMLLNYFFPLGTAL"
     gene            complement(11835..12284)
                     /locus_tag="PLP58_13140"
     CDS             complement(11835..12284)
                     /locus_tag="PLP58_13140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009959176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HRJ09515.1"
                     /translation="MSEHLPSLHPASQDWDGFLNSVIDEFGCTTGTLHRLDAADRHLK
                     LVAHRGIPPQLMPVIQSIPIGKGIAGAAAERLEPVELCNLQTDTSGVAKPGAKQTQVQ
                     GSLAVPVLDGDRLCGTLGIGKLTAYEFTPEEKEKLIAIGREVAARLA"
     gene            12350..12979
                     /locus_tag="PLP58_13145"
     CDS             12350..12979
                     /locus_tag="PLP58_13145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ09516.1"
                     /translation="MNLRDSIFAHLWLLLALPVMAASPAGQAVVGRTVYHPDNSRTET
                     VRDMNVREMTETTYNSANALISKKVFLLNEKGEPLQGNIYDGRGNLVARAQCLYDEFG
                     RRKEDRLSNMKGELFQRIVHEYDASGKALKPKVMNLSQSPTIRPAAIDFTTPPGGVPA
                     GVSPDAGSPSSRFAPTQIPASSQQPASTPSSGPKEEKKPNFFRRLFKKE"
     gene            complement(13125..13721)
                     /locus_tag="PLP58_13150"
     CDS             complement(13125..13721)
                     /locus_tag="PLP58_13150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ09517.1"
                     /translation="MRRRLNPLSERNIKRIDTRPDSAKQTHGFQVCISRNGGLHTKHF
                     SDSLYNGMKGSLKAARAYRDQILSEMPEIEASRIAHSHNAKNKSGHVGISYRKSKNSK
                     GKLVRHIAVSVRAERGKTVSRVYAYTKDNHDEKLAEAIAFREEMLRQRLEREGGLARI
                     VKRQNLAVKKTATGKPAAAPAPRKRTSRKTAASQKRGL"
     gene            complement(13812..14264)
                     /gene="dtd"
                     /locus_tag="PLP58_13155"
     CDS             complement(13812..14264)
                     /gene="dtd"
                     /locus_tag="PLP58_13155"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012420025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacyl-tRNA deacylase"
                     /protein_id="HRJ09518.1"
                     /translation="MRVIIQRSLEASVSIGGEVAGNISHGLVILAGVEDADTEEDAEW
                     LAGKIAAMRIFADEEGKMNRSVIEAGGGVLVVSQFTLHASTRKGNRPGFTRAARPEKA
                     VPLYEHFLAALERQLGARVERGVFGADMRVALVNDGPVTIQMDSRARE"
     gene            14345..>14973
                     /locus_tag="PLP58_13160"
     CDS             14345..>14973
                     /locus_tag="PLP58_13160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009962466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenoylcysteine decarboxylase"
                     /protein_id="HRJ09519.1"
                     /translation="MRFLITAGPTREPLDPVRYLSNRSSGKMGCALASAALEAGHEVV
                     LVSGPVALEPPPGAQITRVETARQMFEAVRDQISGCEAAIFCAAVADYRPVSVAGQKI
                     KKTGERLVLELERTEDILGSARSIFGFRGFLAGFAAETTNLLEHAHDKLRRKGCDLVI
                     ANDVSRAGIGFDSAENEVILCLPGAREIPLPRQTKAGLARAIIAFITET"
BASE COUNT         2593 a         4688 c         4979 g         2713 t
ORIGIN      
        1 ccgggcgcgg tggcggcgcg ggtgccgcag ctcgccaaag cgaaggccga tgtcgagtcc
       61 gcgaaagccc aactcgccaa agcgcgccgc gacctggagc gaaccatcct ccgcgcgcca
      121 tacgcggggc aggtgctgga gcagacggcg gatgtcgggc agatggtggg gccgggcacg
      181 acgctggggc agatttttgc cgtggattat gtcgaggtgc gcctgccgct gccggagcgc
      241 gagagccagt ttttgtcact gccacagcct ttccgagacg gcgcggccag atcgggagga
      301 aaggcgctgt tgaaagccac cgtcgccgga aagcccgcgc aatgggaggg ccgcgtggtg
      361 cgtgtggaga gcgcgctgga cgagacgacc cgccaggtca tggccgtggt gcaggtggac
      421 gatccctatg gccgccgcga ggacggcaca ccgccgctga cgatcggagc tttcgtcgag
      481 gccgagctcg cgggggagcc tctgagggat gtctttgtca ttccacgcgc cgccgtgcgc
      541 gccggcaacg agatcatcct catagaccgc ccgcaaaaca ccctgcggcg ccgcgtcgtg
      601 gagccgctgg tcagcaactc caggcacatc gtggtggaca gcgcctcagt ctccgcgccc
      661 aaggaaggcg atgtactctg cctcacgccc atccccttcc ccgccgacgg cgcgcgcgtg
      721 ctgccgacca tagacggcca ggtggaaagc ccgcccgtgg gcaaaaaggc cgcgaagcag
      781 ccggaggacg gcgagccgaa gccgaaagcg cccgtcgtca gccagaatga cgggaagtga
      841 gcgcgtcccg tgatttctcc ggtgaaaaat tcaccgccgg cgcgttcctg ttccgccatg
      901 aaaaacaccc tgttcatcct tcttctcacc gccctgctca cctcctgctc ggcgggtttc
      961 cggcgtgaat ggaacacagc cctcaagacc ggtccgcagc ccggcgtggc gggcgcctgg
     1021 gagggcacat ggaaaagtca tgtgaatgga catcacggac gcctgcgggc ggttgtctcc
     1081 gcgccaaaaa acgacaccgg cgatcatgac ttccactatc acgccacctg ggcgcgaatc
     1141 ctcagcggag gcttccgcgc ggaacatcgc gtgactcctt tgcgcggcgg cggcagcagc
     1201 ttcaagggca gccaccagat gcccgactgg gccggcggac tctacacgta tgagggcaca
     1261 gtgaaggggg acagctttga ggcatgctac gaatgcggca aagacaaggg agtcttcagc
     1321 atgaggcgcg cgccgcaggc gcggtgatga tttttaatac ctccgccgcc agggcgaaac
     1381 caaacgcgcc ggtgacaaaa gccgcagcgc caaacccggc agcgcaatcc agtcgcagac
     1441 cctgcccgcc tccatcacgt tcaggagagc aggtgccatc cgcttgcgga tagaccgcct
     1501 tttccgtgga atacacgcag ggcactcccc aatccatggg acgcccatcc gtgcttttgg
     1561 caaagccgtg ctccgatcgc agcaggcggc gcacctgctg aagaagcggg tcggccccgg
     1621 ccaggccgag atctgccgcc cggatctgtg tggggtcgcg ccggccgccg gccgagccag
     1681 agacgaccac cggaatgccc agtgcgcggc attttgcgat gatgagcgct ttgatgcccg
     1741 tgtcatccac agcgtccacg atgacgtcaa acccaggcgc aaggatgcgg tccgcgctgc
     1801 ccggcagcaa aaactccgca acggcggcga cccggcattc aggattaatg gcgcgcacgc
     1861 gctcctccag cacgcgcacc ttcggccaac ccaccgtgcc ggcaagcgcg gggagttggc
     1921 ggtttgtgtt tgtgacacag acatcgtcca tgtccaccag agtcagcgca cccaggccgc
     1981 tgcgcgccaa agcctccacg acccaggagc caaccccgcc cacaccgacc acgcacacat
     2041 gcgccgcgtg caggtgcggc agcgccgccg cgccataaag ccggccaatg ccgccaaacc
     2101 tttgaaacca ggattccgtc atgggccttt gacataccca caaagaatgc ccgggccagc
     2161 gcttcgtcag tctggaccgc gctcagcggc ggtttatttc gatgaactgc ccgccaatgc
     2221 cccaggcgat gtcctcgcgc ggaaggatgc cgtcgccgcc aaggtaaaat ttgctcgcaa
     2281 agatgtagtt gtcccgctgg atgccgcctt ttttgccgcc tgagaccagg tccgcgacca
     2341 ccagcccaag cgtgccggcc agtccctcct gctgccgcac atgctcgcgg aaatcggcct
     2401 cgttgggatt ggtgaaagcc agtccggcaa gaacgacgat caggagcagg aggatgagtc
     2461 tcatgcgcgc atcatgacgc cgtgcgactg ttttcaccag caagttttcc gacagatcgg
     2521 ctcacccgcc ggcctgaggc gttgttttaa ctccgctcct gtcccacagc agccacatcg
     2581 ccaccacgcc cagccagggc gccaggccgg ccatcgccat gccgaggtcg aaggacttcg
     2641 tctcatccac cagcttgccg aataatttgt gcatgggcga tgacactgcc caaacccagg
     2701 tgctcaggag tcctgtgagg cgccccacat ggcggtcgga aagctcctgc acgaagctgt
     2761 agtagcaggg gaacagggcc agggcgcccg cgccgacgac caggatggcc gccatcagcg
     2821 cccagccctt cggcagccag agcaccagca ggctgaagga ggtcaaaacg cacgcgccgg
     2881 catagaccag gcgtcttgcg ttgtgcgccg tcatgccgaa gcgccgcgcc agccagagcg
     2941 agatgatgcc cgccgcgacg cagccgatgt ccgtgccgat gtaatacgcc gagttgaacg
     3001 ccagcgcctc cgtctcgctg tagccgcgcc cctcctgcat gaacttcggc agccagacgc
     3061 ggaatagatg ccagcatgtc tgcgctccga agatcaaggc ggcaagcgcc cagaagcgtc
     3121 gtcccagcag gatgcccaac agcccctcga atgcggcttt ttccatcgga gccaccacgt
     3181 ccagctccac cctgcggatc gaggcgaacc acaacaccac ccacacgaga cccgcggcac
     3241 cgatgacctg gaaggccagc cgccagcttg aaacatcctc cgtcatcagc cagatcatga
     3301 tcggcggcgt caccaccgcg ccaatggagg cgccgctttg cagcacgctg ttgcccatcg
     3361 tgcggtcctc cggacgcagc agggcgaatg tggtttttaa tgcgcagggc cagtgcccgg
     3421 cctcgaaaaa cccaagcagc acgcggcaga tcatcagctc cgggtaatcc cgcgtgtgcg
     3481 ccgaggcgat gcccatgagc gaccagcccg ccagcacgat gggatagagc cagtacaccc
     3541 ggaaccggtc cgccagaaac ccgaacacga gtgatcccgc ggcaaatgcc cagccgaaag
     3601 ccagctcaag gtccccatac tgctcctggg aaaggccaaa ctccctggtc acgcgcaccg
     3661 ccgcgttcga cagcgtctgg cggtccatgt agttgatcgt cgtggccagc agcagcaggc
     3721 cgcagacgta ccatttccaa aatgaggggc gtggggcggg catcgccatt ggtacggaaa
     3781 aaatgcgccc gtcacagcgg ctttccgccg aatccttgaa ccgctgcttg aatcttcgtt
     3841 gtatgaaacc accatgaccc gtctttctga ctgcgtcttc cgctcatgat cttcgacgtt
     3901 cattcccatg cctggaactt ccccgccgat ttttcggagg acttcatcac gcaggcgggc
     3961 cgtgccagac caggcgtgaa agtggacctg agcgcgactt acgaagcgta tcgcgcggcg
     4021 gctccagatg acacgcacac gatcgttttc ggcggcaagg caaggctcag cggcctctgg
     4081 gtggatgaca aaaccgtggc ggaatatgtg gggcgagatc ccgcgcggct gaccggcttt
     4141 ttatccgtgg acccgacccg gcccggatgg gaaaatgaaa tgcgcgccgg acacgaggag
     4201 ctgggcctgc gcggcatcaa actgctgccg atgtatgccg ggttcagccc tgatgatccc
     4261 gcgctggagc cgctatggat ctatgcggag gcaaacggac tgccagtgct gctgcacatg
     4321 ggcaccacct tcatcgcgca ggcgccgctg gccttcactc tgccgcgctt gatcgaaccg
     4381 gtggcgcagc gccatccggg agtgaaaatc atcctggctc atctcggtca tccttacgag
     4441 ggcgagtgca tcgtcacgat ccgcaagcac gagaatgtct tcaccgatgt aagcgcgctg
     4501 cattaccgcc cgtggcagtt ctatcactcg ctcatgctcg ctcaggaata cggtgtgtgg
     4561 cacaagctgc tctttggcac ggacttcccc tttaccacgg tgaatgccag catcgggggc
     4621 atccgcgggc tcaatgacat gctcgaaggc acgaggctgc cgaggctcga tgctgagcag
     4681 atcgaggcga tgatccaccg cgacagcctc gcgctgctgg gactgcggca ctgagaaaag
     4741 tgttaccggc cgcacggacg ggcgtttgaa ccgcgtccga atggaaactc cccgcgcaaa
     4801 cagccgccgc catgatctcg acgccctgcg cgcttttgcg atgctgctgg gcatcgcgct
     4861 gcatgcggcg ctctccttca ccggcgggcc gtggatggtg caggacacgc gcacgagcgg
     4921 cttgtttcat ctgatggtgg gcgcggtgca cgggttcagg atgccgctgt ttttcctgct
     4981 cagcggcttc ttcaccgcca tgctttggcg caagcgcggg ctgcgggctt tgctcgcgca
     5041 ccgcttcaag cgcgtgttcc ttccgcttgt gctcggctcg gtgacgattg tgcctgtgat
     5101 gttccttttg ggcggcgtct ccgcgaaatc cggcgcgcgc caggaagagt caaagccggc
     5161 cactctctgg cacgcctgcg cggcgggtga cgaggatctg gtgcgcgggt tcctcggcga
     5221 tggcgcggac gtcaacgccc ccgaccctct gctgggcgcg actccgctca cccatgccag
     5281 cctctttggc cgggaaaatg tggcgcggct gctgctggag cgcggcgctg acgctaacaa
     5341 aaccaaccgc gacggaggca ccgcccttca tgcggccgcg tttctgggca gggctggcat
     5401 cgtgcgcatg ctgctgggcg cgggcgctga tccgcgggtg agaaacgagc gcggagacac
     5461 ggcgctcgat gccgcgcgga ctgacttgcg cgccacgcag tttctggccc gcatcctccg
     5521 ggtgaatgtg gaaatggacg ccctgcaggc tggacgcgca gagacgcgcc gcctgctcgg
     5581 cggggatgcc agcgaggaga aaccgaggcc gcttcttgga gtctggctgt ttttgcggca
     5641 catgccggtc ttcgcgcatc tttggtttct ctggttcctg tgctggatgt tcgccggttt
     5701 tgccatgtgc gcgcgactgg cggacggcat cggctggcgg cccgctccgc gcgcacgggt
     5761 cacctccgca tggcggtatc tctggctggt gccgctgacc ctgatcccat cctggttgat
     5821 ggcccaggcg gggcagaatt tcggaccgga aacctcgtcg agcatcctgc cgcccgcgca
     5881 tctgctggcc tattacgcgg tcttctttgg ctttggcgcg ctgtggcatg acagcatgga
     5941 cacggccggc cgcctgggcc ggtgctggtg gctgacactg cccctggcct tgctgctggt
     6001 ttaccccgcc gggctggcgg ccactttggg cggggccaag ctgcgcggcg cggcggtgct
     6061 gctgcaggtg ctttatgcct ggctgatgtg cgtggcgatg atcggcctgt tccgccgctt
     6121 tgtgtccggg gagcgaggat gggtgcgcta tctttccgac gcctcctact ggatgtacct
     6181 ggcgcatctg ccgcttgtcg tcgccgcgca gatgtgggcg cgtgactggc cgctgccagc
     6241 gggaatgaag ttcctgctcg tctgcgcggg agtcttcgca ctcctgcttg ccagctaccg
     6301 ctggcttgtc cggcacacat ggatcggcgc gctgctcaac ggacgcaagg agcgccctcc
     6361 catgtctcaa cccgctgtca cgggatcacc gtaaagcgtg aagtggtggt ggctgagcac
     6421 gcgcacgtcg aaaatctcgc cggtgtggcg ctccgcgctg ccttcgaaga cgacgatctt
     6481 gttggtgcgg gtgcggccca tgaggcgggt ctcggtggtc ttgctggggc cttcgcagag
     6541 gatttccaca ttttgtccca ccagctcatc gtagcgcggc agggcatggg agttgaccag
     6601 ggccagcaga tcctggttgc gctcctcctt gacccgttcg ggcacctgga cggactcgtc
     6661 catctccgcg gctggcgtgc cgcgccgctt cgaatagcgg aagacgaagg cgttgtcgaa
     6721 gcgcacccgc tcgaacagcg cgcgagtctc ctggtaatgc gcgtccgttt cacccggaaa
     6781 gccgacgatg acatccgtgg tgaccgcgat gcccggacgc gcggcgcgga tgcgctcgac
     6841 gaggtcggca aacttccccg ccgtgtacgg acggtgcatt tttttcagaa tctcatcact
     6901 gccgctctgc accggcagat gcacatgctc ggccagcttg ggcaggcggg tgaaggcctc
     6961 gatgaggtcc ttcttgtagc cgatgggatg cgggctggtg aagcggatgc gctgaatgcc
     7021 cggcacctcg tggacggcct caagcaactg cacgaacggg cttttgccat ccacgactgg
     7081 aaactcgtgc cgtccgtaga ggttcacgat ctggcccagc aacgtcacct ctttgacgcc
     7141 ccggtcggcg agacggcgca cttcctcgac gatgccggca atgggccgcg cacgctcgcc
     7201 gccgcgcgtg tagggcacga tgcagaaggt gcacttcatg ttgcagccct gcatgatgct
     7261 gacaaaggcg ctgccctgcc tttccttgag ggtgtggtcg cggatggtgt cctgcgagcc
     7321 ttcctcctcc cccacatcca cgatggaata gcgctcgtca tccatctgcc gctcctcttt
     7381 ggcccggatg atccggtcca cataatccac cacgcggtgg tacttctgcg tgccgaccac
     7441 gaggtccacg ccggattttt ccaccagctc cggcccccgg ctctgtgcca tgcagcccat
     7501 gaatccagtg accaatggca cgcgccgccg ccgcgccatg cccatctttc ccagcgcctt
     7561 ctgctccgcc tggtcgcgca cggagcaggt gttgatgagc accacatccg cgtcctcatc
     7621 ggtgggcgtc agggtgtagc cgcgctcgac gaacatctgc gagacctgct cggtgtcgcg
     7681 ctcgttcatc tggcagccgt aggttttgat atagactcgg ggcatgaggt ggcggccaga
     7741 atacgggcat aacggctttt gacaaccgct ccaggccgga cgggcggggt tcatggccag
     7801 gcttgcgcgc gcctcctttc cgcttatgga gcggccctct tatgcaccat tttcattact
     7861 cccagggcgg gctgtttgtc gaggacgtgt ctctccagtc attggcggaa aagcacggca
     7921 cgccgctcta tgtgtactcg aagggcacca tcaccagtca tttcacccgg ctggatgccg
     7981 cgctctcagg actggaccac ctcatctgct atgcggtgaa ggcgaactcg aacctggccg
     8041 tgctggccac catcgccagg ctcggcggcg gctttgacat cgtctcgggt ggcgagcttt
     8101 accgcgtcat caaggcggga ggtgatccgc gcaaatgcac ctttgccggc gtgggcaaga
     8161 cgcgcgagga gatcgcctat gctctcgacc agggcatcta ctgcatcaat gccgagtccg
     8221 aggccgaact gcggcacatc aaccagatcg ccggggagct gggcaaaaaa gctcccgtgg
     8281 ccgtgcgcgt gaaccccaac gtggacgcga aaacccacgc caaaatcacc accggcaaga
     8341 gcgagaacaa gttcggcgtg gactttgaaa acatccttga tgtctatgcc cgcgccgccg
     8401 ccgaatgcca gcacctggag ctgcgcggcc tgcaaatgca catcggctcc cagcttacca
     8461 gcgtcgagcc cttcgtcgag gccgtgcgca aggtcgtgcc cctcgtccag gaggtgaagg
     8521 cgcggcatgg catccagttt ttcagcatcg gcggcggcat cggcatcaac taccgccaga
     8581 gcctcgactc cggccccgag agctggtggc aggaaaatga aaacctgcat ccgctcacca
     8641 tccaggccta cgcggacgcg gtgacgcccc tgctccagcc cctcggcctg cgcattctct
     8701 gcgagccggg ccgcttcatg gtcggcaacg cgggcgcgct catcacccgc gtgctctacg
     8761 aaaagcgagg cgcggccaag atattcaaga ttgttgacgc gggcatgaac gacctcatcc
     8821 ggcccacgct ctacgagggc tggcaccaga tcgtgccgct caagcagccc gccacaggag
     8881 aagtagagaa ggcggacatc gtcggtccta tctgcgagac gggagacttc ctggcacagg
     8941 accgcgagct gccgcccgtg caggcggggg actacctcgc cgtgctcagc gccggcgcgt
     9001 acggcttcac catggcctcc aactacaaca cccgccccat gcccgcagaa atcctcgtgg
     9061 atggagccaa agcctccgtc gtgcgcgagc gccagactct ggatgacgtg ctcaaggggg
     9121 agcgggtgga gtaaaagcag cgcccggtct cagcgtccga acagaggacg cggcccctga
     9181 cgcacagggc cgggctgcgc ttcctccttg gcgcgctcgc gtgcggcgct gtcgcggtag
     9241 aagaggacgc cgttgcgctt gaggttggcg atgaggtcgt gcaggtcggt cttgagcgcg
     9301 gggtcgttca tgagggcggc gaggatgcct ttgccggagt tgaggttgcg cgcggtgagg
     9361 gcgaggctgt cggcggcgct tttgatggat tggagggtgt ccccggcgct ggtgacgacc
     9421 ttgtccactt tggcgatggc gggttcgagc ttgtcgagca tgaggccgat cttggcggtg
     9481 ccttcctcga cgtttttcga ggaggtcttg aagctggcgg cggcggtttg gaggtcggcg
     9541 agggtggact tgagcatgcc ggcgttttcg tcgccgagca ctttttcatc cacgcgcttc
     9601 atggtctggc tgaggtgctc catgctgtct ttgaagtcgg tgatggtggc gtcggagagg
     9661 gcgtctttgt tgatcttgga gacggattct cggatgtctt tgagggcggc gcggaggtcg
     9721 tcgaggacga ggtccacttt tttgccgact tcctcggcgg tgctctggag gtcggagagg
     9781 ccgccgcttt tgacgccttc gatgatttcg gcgtggtcgt gggggatgaa ggcggtggcg
     9841 ccttcgctgc cggggcggat ctcgatgagg gcgtcgccca tgaggccggc ggagccgatg
     9901 gcgaaggcgg agtcggcggg gatgtccaca tcctcgttga tcttgaggtc cacgatgatc
     9961 ccggtgaagg tttcgttgag ctgggggtgg cggaggactt tgccgacttt ggagccgccg
    10021 agatagacgg gggagccttc cttgatgccg gaggcgttgg ggaaggagac gcgcaggcgg
    10081 tagctgtcgg tgaaccattc gcgcaggccg ccgaattgca gaatgatgcc gccgagcatg
    10141 agcatgccga cgaagaggaa gaggccgacg aggacttcgg ttttgcgttc ggaattcatg
    10201 gacgggggtc aggcgatgac gccggagcgg ccggcgatga agttctggat ttctggttcg
    10261 ggggaggcgc gcagttcgcc gggggtgccg ttgaagctga caacgccgtt tttgagcatg
    10321 acgacgcggt cggcgatgcg gaagacgctg ctcatgtcgt gggtgatgac gatgctggtg
    10381 acgcggaagc gcttttgcat gcgctggatc atgtggtcta tgacgtcggt gacgacgggg
    10441 tcgaggccgg cggtgggctc gtcgtagagg atgcactcgg ggcgggtgac gatggcgcgg
    10501 gcgatgccgg cgcgctttct catgccgccg gagaggttga cgggcatttt gtccttgtgg
    10561 gcggagagtt cgacgaggtc gagggcttcg tggacgcggt cggcgatttg tttttggtcg
    10621 cgcaggccgg cttccttgag ggggaaggcg acgttttcgg cgacggtgag gctgccgaag
    10681 agggcggcgt tttgaaagag gtagcccatg cgctggcggg cgatgaggag ctggcgctcg
    10741 gtcatggagc agaggttgac gccgtcgagc tcgacgcggc cctcgtcggg gtggaggagg
    10801 ccgatgatgt gcttgaggag gacgcttttg ccctcgccgc tggggccgat gagcacgagg
    10861 gtctcgccgt ggcagatgtc gaggtccacg ccctggaggg tcttttgggc gccgaagctt
    10921 tttttcaagc cggtgacgcg gatgaagggg acgctggagt cggtgccggg catgggtcag
    10981 agggcggtgc cgagggggaa gaagtagttc agcagcatgg tgaggaagaa attcaccacg
    11041 agcagggcca gggaggagaa gacgacggcg cgggtggtgc cgaggccgac gccgacggcg
    11101 ccgttggcgg cggtcaggcc ctggtggcag gagatgagca cgatgaggat gccgaagaag
    11161 aagcccttgg tcatgccgaa gatgaggtcg gtgtggttgg tgtggtccac gacgtgggtc
    11221 cagaaccagg tgaaggggat gtggaaggcg aggctggaga cgagggccga ggcggcgagg
    11281 ccgaagacga tggactcggc gatgagcagc ggcatggaga tcatcatggc gaagaagcgc
    11341 ggctgcacga ggtagtccac ggggtggacg cccatgacgc gcagggcgtc tatttcctcg
    11401 ttcaccttca tggtgccgat ctcggcggcc atggcggcgc cgacacggcc ggtgaccatg
    11461 aggccggcga gcacgggggc cagctcgcgg cagagggaga cgctgacgat gccgccgatg
    11521 gtggtctcga tgccgaagtc tttgaattta aaatacgact gtgctgtgaa caccgcgccg
    11581 gtgaaggctc cggtgaccac gacgacggcc tgcgagccgt agccaatgcc gacgatttgc
    11641 tcggccattt gcagaatgcg cagtctgccg cggaagatgg agtgggcgag gtcggccacc
    11701 agcctgccca actgcccgag gtaactcagg aagttcaaaa acgagcgccc aggcagggcc
    11761 agcatctcgg caaacgcgga catcggacgc gcatcatgcc acgccctggg gggatggcaa
    11821 cccgcgcgcg gtgctcaggc cagccgagcg gccacttcgc ggccgatggc gatgagtttt
    11881 tccttttcct ccggcgtgaa ctcgtaggcg gtgagcttgc cgatgccgag cgtgccgcaa
    11941 agccggtcgc catcgaggac gggcacggcc aggctgccct gcacctgggt ctgcttcgcc
    12001 cccggcttgg ccacgccgct ggtgtcggtc tggaggttgc acagctccac gggctccagc
    12061 cgctccgccg ccgctccggc gatgcctttg ccgatgggga tggactggat gacgggcatc
    12121 aactgcggag ggatgccgcg atgggctaca agcttcaaat gccggtcggc ggcatccagc
    12181 cggtgcaggg tgccggtggt gcagccgaac tcgtcaatga cgctgttgag aaagccgtcc
    12241 caatcctggc tggcgggatg gaggctgggg agatgttcgc tcatgacggg aaatgatctt
    12301 ggcttggcgg cggggcggga ataggatggg gcgtattttt ccaggattca tgaatctacg
    12361 cgactccatt tttgctcatt tatggctgct tttggcgctg ccggtcatgg ccgccagccc
    12421 ggcgggtcag gccgtcgtcg gccgcacggt ttaccacccg gacaactcac gcaccgagac
    12481 cgtgcgcgac atgaacgtgc gggaaatgac cgagacgacc tacaattcgg cgaacgcgct
    12541 catctcgaaa aaagtcttcc tgctcaacga aaagggcgag cccctgcaag gcaacatcta
    12601 cgacgggcgc ggcaacttgg tggcccgcgc ccagtgtctt tacgacgagt tcggccggcg
    12661 caaggaggac cggctgtcga acatgaaggg cgagcttttt cagcgcatcg tccacgaata
    12721 tgacgccagc ggcaaggcgc tgaagcccaa ggtgatgaac ctctcgcaaa gcccgacgat
    12781 ccgcccggcg gcgattgact tcacaacgcc gccgggcggg gtgcccgccg gtgtgtcacc
    12841 cgatgcgggc agcccttcct cgcgtttcgc cccgacgcaa atccccgcct cctcccagca
    12901 gcctgcgtcc accccgtcct ccggtccgaa agaggagaaa aagccgaact tcttcagacg
    12961 gctgttcaag aaggagtgat cgttcgttga cccggcgtcc ggcacgcgcg caaggtgtgc
    13021 gtgcgacaca ggcattcttg tctgttcgga gcgggcagtg ttcccaggtg gatcagggcg
    13081 gacaagagcg tccgcgtcac gcatggcgtg ctttaacgcc cgcgtcagag cccgcgcttc
    13141 tggctggcgg cggttttgcg ggaggttctc ttccttgggg cgggcgcggc ggcgggtttg
    13201 ccggtcgcgg tcttttttac cgccagattc tgccgcttga cgatcctggc cagccccccc
    13261 tcgcgctcca gacgctggcg cagcatctcc tcgcggaagg cgatggcctc ggccagtttt
    13321 tcatcgtggt tgtccttggt ataggcatag acgcgggaaa ccgtcttgcc gcgctcggcg
    13381 cgcacggaga cggcgatgtg acgcacgagc ttgcccttgg agttcttgga cttgcggtag
    13441 gagatgccga cgtggccgct tttgtttttc gcgttgtggc tgtgggcgat gcggctggcc
    13501 tcgatctcag gcatctcgga gaggatctgg tccctgtagg cgcgcgcggc ttttagggag
    13561 cctttcatgc cgttgtagag gctgtccgag aaatgcttgg tgtggagtcc gccgttgcgc
    13621 gagatgcaga cctggaaacc atgcgtctgc ttcgcggaat cgggccgggt gtcaatgcgt
    13681 ttgatgttgc gttcggaaag gggattgagt cggcggcgca ttcaggaatc agaggttttg
    13741 ggggggagcc tcaagtttgg acggtggcaa cctgcgcgca agatgaaagt gcctttgcag
    13801 ccatgcgcct gctattctct ggcgcgcgag tccatttgaa tggtgaccgg tccgtcattg
    13861 accagcgcca cccgcatgtc cgcgccgaag acgccgcgct ccacccgcgc gccgagctgc
    13921 ctttccagcg cggccagaaa gtgctcgtaa agcggcacgg ccttctccgg ccgcgcggcg
    13981 cgcgtgaagc ccggccggtt gcccttccgc gtgctggcgt gcagcgtgaa ctggctgacg
    14041 acgagcactc cgccgccagc ctcgatgacg ctgcggttca ttttcccctc ctcgtccgcg
    14101 aagatgcgca tggcggcgat cttccccgcc agccattccg cgtcctcctc cgtgtcggca
    14161 tcctccaccc cggccaggat gaccaggcca tgggaaatat tccccgccac ctcgccgccg
    14221 atgctgacac tggcttccag ggagcgttgg atgatcacgc gcatggggcg cgatgatgga
    14281 gagggcgcgg cttttgtcca cgcgcgcgtt gacggcggcg gcccgcgcgt tgaatggaaa
    14341 ttccatgcgc tttctcatca ccgcaggccc cacgcgcgag cctctcgacc cggtgcgtta
    14401 cctctcgaac cggtcctccg gcaaaatggg ttgcgcgctg gcctccgccg ctctggaagc
    14461 cgggcatgag gtcgtgctcg tcagcggccc tgtggcgctg gagccgcccc ccggagcgca
    14521 aatcacccgg gtcgagacgg cgcggcagat gttcgaggcg gtgcgcgacc agatctccgg
    14581 gtgcgaggca gccatcttct gcgccgcagt ggctgactac cggcccgtct ccgtggccgg
    14641 gcagaaaatc aaaaagaccg gcgagcgcct cgtgctggag cttgagcgca cggaggacat
    14701 cctgggttcc gcgcgctcca tcttcggctt ccgcggtttt ctggcgggct tcgcagcgga
    14761 aaccacaaac ctgctggagc acgcccatga caaactgcgg cgcaaaggct gcgaccttgt
    14821 catcgccaac gatgtctcgc gcgcgggcat cggctttgac agcgcggaaa acgaagtcat
    14881 cctctgcctt cccggcgcgc gtgaaatccc cctcccgcgc cagacgaagg ccgggctcgc
    14941 gcgcgcgatc atcgccttca tcacagaaac cca
//
LOCUS       DAOMYJ010000121        14925 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Prosthecobacter sp. isolate AMR_MDS_5629
            NODE_1364_length_14925_cov_20.203026, whole genome shotgun
            sequence.
ACCESSION   DAOMYJ010000121 DAOMYJ010000000
VERSION     DAOMYJ010000121.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867850
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prosthecobacter sp. (wastewater metagenome)
  ORGANISM  Prosthecobacter sp.
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Verrucomicrobiaceae; Prosthecobacter.
REFERENCE   1  (bases 1 to 14925)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14925)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.30
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:34:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,742
            CDSs (total)                      :: 4,684
            Genes (coding)                    :: 4,676
            CDSs (with protein)               :: 4,676
            Genes (RNA)                       :: 58
            tRNAs                             :: 55
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14925
                     /organism="Prosthecobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1364_length_14925_cov_20.203026"
                     /isolate="AMR_MDS_5629"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1965333"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..516)
                     /locus_tag="PLP58_13165"
     CDS             complement(<1..516)
                     /locus_tag="PLP58_13165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ09520.1"
                     /translation="MSETTAAPATGRFIRAALVIFPAGTIILCILSFGIWWWKRERVE
                     QRGYQYALALRRDLSESGLSRHSSILHEALSRADPQRFQAVAAYLESSMGAENMGYHV
                     QRGQRVRVGDMEVSDIDVELTGRQRPREVVLLLVPYGFAARAAEESRALAMMMSLAHA
                     ITGEARVRTLRL"
     gene            653..2203
                     /locus_tag="PLP58_13170"
     CDS             653..2203
                     /locus_tag="PLP58_13170"
                     /inference="COORDINATES: protein motif:HMM:NF015585.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialate O-acetylesterase"
                     /protein_id="HRJ09521.1"
                     /translation="MRTPALISLLAALALQARAELKLPAIIGDNMVLQQKQANPLWGW
                     DTPGTGVTVKFAGQTKSATAGADGKWTVKLDPLPANANPAVLSIQGSTKRDLQNVLVG
                     EVWICSGQSNMQWSVNASWDFELETATAKHPLIRLISVPQVGTQEPQQDFKGEWKECS
                     PQTVGGFSAVGFFYGRVLHQVLGVPVGLINNAWGGSAAEAWVRRDVLEKDARFKGLIE
                     GWVAREKQLASEEARLAHEKAMAAWKEKADAAKAAGQPFTQRAPQSPQATLAGNARPG
                     NIYNGVLLPTIGYGVKGAIWYQGESNASRAHEYGHLFPLMIQHWRDEWKQGDFPFYWV
                     QLADFKAEKPEPGDSDWAELRESQTKTQNAVPNSGQAVIIDLGEANDIHPKNKRDVAE
                     RLARIALARDYGMDIRWRSPEFKAAEFKEGKAIVTLDTFGSSLRTVDVSEVKGFALCG
                     EDRKWVWAEAKIAGRDKVEVSAKTVPNPVAVRYAWADNPVCNLFSIEGLPVTPFRSDD
                     FPMITAPK"
     gene            2344..2544
                     /locus_tag="PLP58_13175"
     CDS             2344..2544
                     /locus_tag="PLP58_13175"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009959829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HRJ09522.1"
                     /translation="MKAELVEQALHIVKDPPILINMVSKRVKQLTTGRAPLVERRAGL
                     READYALLEIIQGKITTTDLEE"
     gene            2578..3045
                     /gene="smpB"
                     /locus_tag="PLP58_13180"
     CDS             2578..3045
                     /gene="smpB"
                     /locus_tag="PLP58_13180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018971026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HRJ09523.1"
                     /translation="MSEEIATNRKALRDYHILDRYEAGVELRGTEVKSIRQGRLNITD
                     AFARVERGQVWLYNMDIQPYDKASFEQHEPRRARRLLLHKREILKLFAHTEQKGLTLP
                     VLRAYWKGSRIKVELGVGKGKTKGDQRIDLKEKAVRRETQRVVSSFNRKGKQG"
     gene            complement(3052..3729)
                     /locus_tag="PLP58_13185"
     CDS             complement(3052..3729)
                     /locus_tag="PLP58_13185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007417289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRJ09524.1"
                     /translation="MPASSSPLIAQDVHRTYVLGGHELRVLRGVELEVAAGEKVFLCG
                     QSGAGKTTLLYVLGGLEKPTRGDVLVHGKSLYHGPAAARARARNQTMGFVFQHYFLLP
                     ELTALENVLLPAMIGGRKAGDRARELLDRVGLAGRMDHLPTELSGGEQQRVAIARALI
                     NDPGILYADEPTGNLDAGTGAGVMDMLMGVVGEAGKTLVVVTHDLALAKRGDRLLRLK
                     DGGLVPG"
     gene            complement(3765..4685)
                     /locus_tag="PLP58_13190"
     CDS             complement(3765..4685)
                     /locus_tag="PLP58_13190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009959202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HRJ09525.1"
                     /translation="MSQEAPLIAAIEAGGTKFVCGIGRDPHQLLDVIRIPTTTPAETL
                     EAVSHWLSKMKVAHGPLAAIGVGSFGPVDLNPASETYGSITTTPKPGWQNTDLVNFFK
                     NRFKVPVGFDTDVNAAVLGEYLWGAGQGMDPLVYLTVGTGVGGGVLVHGRLLHGLMHP
                     EIGHLMVPPPQNSLAVQREGFCPFHKHCVEGYVSGPALAHRWGVRADSLPADHPAWEE
                     VACTMGHALMNLTLALCPRRIILGGGVMSQPHLVPLIRGKLRQHMNGYLSVPQFGRDL
                     EEFVVPPGLGARSGLLGALALGKMAFDARH"
     gene            complement(4682..5602)
                     /gene="metF"
                     /locus_tag="PLP58_13195"
     CDS             complement(4682..5602)
                     /gene="metF"
                     /locus_tag="PLP58_13195"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009959205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004489 - methylenetetrahydrofolate
                     reductase (NAD(P)H) activity [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase [NAD(P)H]"
                     /protein_id="HRJ09526.1"
                     /translation="MHIADILSQQKPTLSFEFFPPKTADASEALYQTIGELEALQPSF
                     VSVTYGAGGSTRELTHDLVVRIQKTTRLDPVPHLTCVCHSEGDIQAILERYAQAGVSN
                     ILALGGDPPRNLEGYERKNDAFQHAADLVRFIKKFSDSGAHPDRRGFGIGVAGFPEGH
                     PATPNRVLEMEHLKAKCDEGADYICTQLFFDNHDFLDFRDRCRLAGVHIPIIAGIMPI
                     TSAAGMRRMAELAAGARYPAKLLRAIQRCGNDEEAVRRVGVHYATEQCLDLLGHGVDG
                     IHFYTLNKSQATREIYASLGIRDSADVRKP"
     gene            complement(5659..6831)
                     /locus_tag="PLP58_13200"
     CDS             complement(5659..6831)
                     /locus_tag="PLP58_13200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ09527.1"
                     /translation="MSAALHLAFLHVACQRAQTWLLAGVLGLMMAVPFSLRVLLSAAG
                     DQMRSRAELTPQVLGARGSALDLLFTALYFKRQPLPRIEMRVVREARDAGQGSVIPLH
                     ARFHCQGAPVVGTELDYFEFRGLRLDGGRWFARLGDCVVGARVAAERGLKPGGNVFTS
                     QEQVFDLAGSHPLKMRVTGVLALSGTADDGAVFADIKTAWLIEGLAHGHDDLAEEDTL
                     REEAGNTVANAALRTFQEVTDENLGSFHFHGDPDSFPVSAAVVLPRGKKAEAILAGRY
                     LDERLPVQLIRPLDEFQSLMDTLFRLERLVLMVLGLVSAGALAAAALVFALSFRLRRR
                     EFRTLEDIGVPRSSLLASRALEILLTGALGVVIAILLASLAAWRAGEWAALLLAGG"
     gene            complement(6828..7484)
                     /locus_tag="PLP58_13205"
     CDS             complement(6828..7484)
                     /locus_tag="PLP58_13205"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HRJ09528.1"
                     /translation="MASLSAENLRFRHPGGGFQLCLESVEAGPGKALAVLGPSGSGKS
                     TLLRLLAGLLSPGAGRVCVDGEDLRAMTPAQTRALRLRRMGLVFQDFALLDHLTVAEN
                     LLLPARFLGLCMTGARERARELAERLEVASFWDRPAARLSQGERQRVAVARALAHGPD
                     LVFADEPTASLDSGRCGLVTDLLLRTCRESGGVLVMATHDARAAAIFPANLNLGDARA
                     "
     gene            complement(7524..8441)
                     /locus_tag="PLP58_13210"
     CDS             complement(7524..8441)
                     /locus_tag="PLP58_13210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009961036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HRJ09529.1"
                     /translation="MIKLTGIADEAGASLDVQIRAHKQLGWDSIEMRVVELDGVKGNL
                     HEVPEAAFDKICEKLAQAGMGVSGFGSLIGNWAKKITDDFSITEAEINRAIPRMQRLG
                     TKLIRVMSYAVCKNDDGSDAAEQHVDERVRRMREINKRFADAGLTVVHENCMNWGGMS
                     PAYVRRMMEAVPEMKWVFDTGNPVFIDDRDRPGHKQDSWEMYQAVKPNMVHVHVKDGR
                     WIKEKGDAEYTFPGEGDGQTERIMKDLVESGYNGRISIEPHVAVVFHGAGAADDLSPD
                     EKARLQYDSYIKYGTMLEAMLRRLGAPLA"
     gene            complement(8478..10334)
                     /locus_tag="PLP58_13215"
     CDS             complement(8478..10334)
                     /locus_tag="PLP58_13215"
                     /inference="COORDINATES: protein motif:HMM:NF013440.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GreA/GreB family elongation factor"
                     /protein_id="HRJ09530.1"
                     /translation="MNSEAQKLVAAGKLAAADGEKLSQLEPGTFCRHKSWGVGKVAEW
                     DLLGDKIILDFEGKPGHGLKISFAITSLEVLPQDHFLARRLADLEGLKQMARENASAL
                     VEIALKSAKGGRMLLGELENLLKPHVLTEGEYKKWWEGAKKTLKNARHIVVPAKRTEP
                     LILRSSEEKAGDGMIRTYLGARDMKGKLAALALIQKDLDLFEDPARELLPALQDISET
                     VRKAWRLHLKDSLQIILLRDEVIESIQGAALPLGSMKIADLISEARAQLTEAVGGLPV
                     SLLGRMYRAFPDAFPERAWVPEILNHLTRTGGRAVAEIATVLDANDELDVLAEFLKKA
                     VRNRALTTDLLIWICKERKGLAESVFDIDLANAILDAIEHDHVQGGPKRTGRLSDLLS
                     DDKTLIHELVIETEEDEVRRFAKRIINTSVFDELTRRSLMARIIKARPEMEVLMAENA
                     GPREDNSLIVSWESLERKKKELEELVTVKIPQNKHDIQIARAEGDLRENGGYKAAKEQ
                     QSVLLRLQNKMERELRLARGTDFAGAATDKAGIGTIVDYEAPGGGRTETLTILGAWDG
                     DVENNIVSYLSEIAKAFIGKAPGDTAEIPSESGAPKPVKILAVRPFNTSAAA"
     gene            10485..10904
                     /locus_tag="PLP58_13220"
     CDS             10485..10904
                     /locus_tag="PLP58_13220"
                     /inference="COORDINATES: protein motif:HMM:TIGR04073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exosortase system-associated protein, TIGR04073
                     family"
                     /protein_id="HRJ09531.1"
                     /translation="MKNRIALTILSGLTLCGMALADIQSPPGHHYNWSRKLSRGVGNL
                     LYGWTEPFTVWQRVNRAEGAHAAASDFFIEGFKRTVVRLGYGLYEVATFPQPTWKCTY
                     RPPYYKKEVLDPWWGYTEFSPQIGFASHMDYTRVQGW"
     gene            complement(10961..12370)
                     /locus_tag="PLP58_13225"
     CDS             complement(10961..12370)
                     /locus_tag="PLP58_13225"
                     /inference="COORDINATES: protein motif:HMM:NF015013.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid II flippase MurJ"
                     /protein_id="HRJ09532.1"
                     /translation="MPAGAQGIRYRTLHNGRGSILSSLRALAGRGAGSRMVSGFLLVA
                     VLTIVAKVAAFLKDAAVARFFGTGDELDAFLMAFGLLAFACSLAGGALPEAFLPVYAG
                     LRNRTGFRRAQRFAVQSAVLHGGILLVMTGAAWLAAPGLILRAARGFPPEKQELAVAM
                     LRGLLPFLLCFGMSFQLAAWLRAEKRFAAATSAPMLVPLTMLVLLLAQGAEADVWTLV
                     HGTCLGAALHLMVLAACTAGGLRLGMRRAFLPDPGLRQVRRSTAHYLLAGVVFSSAVV
                     VDQIMASWLEPGSVAVLGYSEKVCSIILAVTAAPAADVLFPYFADKVARQDWEGVRRQ
                     LLASAGLIISLALPAALLLCWLGPWVVGLLFERGSFTEDDTRRVGGVLRYAALQIPFY
                     ILGGLASRVVVAMQATRFVLMLSFLGLAGNAALNWLLMWRMGVAGIALSTVLVQMAAS
                     LLACRYVLAQIRRKLRAAE"
     gene            complement(12411..13649)
                     /locus_tag="PLP58_13230"
     CDS             complement(12411..13649)
                     /locus_tag="PLP58_13230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007417873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HRJ09533.1"
                     /translation="MRSNQWRKLRGLLMHLATTEGFYARKFREAGVRVTSMGRLEEFV
                     AQTPFTTKEELLADRLEHPPFGTNLTRHPAQYTRFCQTSGTSSGQPMAWLDTQESWEA
                     MLECWRRVYEAAGLAKGRDRIFFAFSFGPFLGFWTAFEAAARDYLALPGGGLSSQARL
                     EMMARYGATVLCCTPTYALRLGEMIGEASGVERMALRVTKIIVAGEPGGSIPEVRARI
                     EKLWDASVHDHHGMTEVGPVTFESADLPLCLQVIEDAYLAEIIDPATGREVEEGTQGE
                     LVLSTLDRAACPLLRYRTGDWVRKRMVNNRLMLEGGVLGRLDEMVVVRGVNIYPSAVE
                     AVVRQFPEVAEFMVEQRKVDDMDEIELLVEAPEAVSKSLLKRLENKLRDTFSLRIPAS
                     LALPGSLPRHEFKARRWRKV"
     gene            complement(13756..14430)
                     /locus_tag="PLP58_13235"
     CDS             complement(13756..14430)
                     /locus_tag="PLP58_13235"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009959980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004750 - D-ribulose-phosphate 3-epimerase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HRJ09534.1"
                     /translation="MTHETPLILPSLLAADWSRIGAEVGRAESAGARWLHLDVMDGAF
                     VDNISFGPQMVRTVRGLTGMYLDVHLMIHRADHYLERFLQAGADNITIHAEARYETSV
                     AETLRRIRAAGRHCGLALHPDTPFEAALPFIAEIDLLLVMTVVPGFGGQPFMEKETMP
                     KLAQARDYRAAHGLSYHLEVDGGIYAHTAPVAKAHGANLFVCGTSFFGPADAGAAMAE
                     LAGSVA"
BASE COUNT         2756 a         5058 c         4659 g         2452 t
ORIGIN      
        1 caggcgcagg gtgcgcacgc gcgcctcgcc ggtgatggca tgcgccaggc tcatcatcat
       61 ggcgagcgcg cggctttcct ccgccgcgcg cgcggcaaag ccatacggga cgagaagcaa
      121 gaccacctcg cgcggacgct gtctgccggt gagttcgaca tcaatgtccg aaacttccat
      181 gtcgccgaca cggacgcgct ggccgcgctg gacgtgatat cccatgtttt ccgcgcccat
      241 ggaggactcc aggtaggcgg ccacggcctg gaaccgttgc gggtcggcgc ggctgagcgc
      301 ctcgtgcagg atgctggaat ggcggctgag gccggactcg gagaggtcgc gacgcagggc
      361 aagggcgtat tggtagccgc gctgctccac gcgctcgcgc ttccaccacc agatgccaaa
      421 ggacaggatg cacaggatga ttgtcccggc ggggaaaatg accagggcgg cgcggatgaa
      481 gcgtccggtg gcgggtgctg cggtggtctc agacatggga aagggggata cgacactcag
      541 gagctgccgg ccagtgtttt caacgcgcgg cctgcccgcg ccgggaacgc tccaactccc
      601 tcatgatagg atcgcctgcg gacatgcgtc ttttacgccc cttcccaaca tcatgcgcac
      661 tcccgccctc atctccctgc tggccgcgct cgccctccag gcgcgcgccg aactgaaact
      721 ccccgccatc atcggcgaca acatggtgct gcagcaaaag caggccaacc cgctctgggg
      781 ctgggacact cccggcaccg gggtgacggt gaaattcgcc ggccagacca agtcggccac
      841 agcaggcgcc gacggcaaat ggaccgtcaa actcgacccg ctccccgcaa acgcgaaccc
      901 ggcggtgctt tccatccagg gcagcacgaa gcgcgacctg caaaacgtgc tcgtcggcga
      961 ggtgtggatc tgctccgggc agtcgaacat gcagtggagc gtcaacgcct catgggactt
     1021 cgaactcgaa accgccaccg ccaagcatcc cctcatccgc ctcatctccg tgccgcaggt
     1081 cggcacccag gagccgcagc aggacttcaa gggcgagtgg aaggagtgct cgccgcagac
     1141 cgtgggcggc ttcagcgccg tcggcttctt ttacgggcgt gtgctgcacc aggtgctcgg
     1201 tgtgcccgtg ggcctcatca acaacgcctg gggcggcagc gccgcggagg cctgggtgcg
     1261 ccgggatgtg ctggaaaaag acgcccgctt caaaggcctg atcgaaggct gggtggcgcg
     1321 tgagaaacag ctcgcctccg aggaggcgcg gctcgcgcat gaaaaagcca tggccgcctg
     1381 gaaggaaaaa gccgacgctg ccaaggcggc cggccagccc ttcacccagc gcgccccgca
     1441 aagcccgcag gccaccctcg ccggcaacgc ccgccccgga aacatctaca acggcgtcct
     1501 gctgccaacc atcggctacg gcgtcaaagg agccatctgg taccaaggcg agagcaacgc
     1561 ctcccgcgcc catgaatatg gccacctttt cccgctcatg atccagcact ggcgcgatga
     1621 gtggaagcag ggcgacttcc cgttttactg ggtgcagctc gccgacttca aagccgagaa
     1681 acccgagccc ggcgacagcg actgggccga gctgcgcgag agccagacca agactcaaaa
     1741 cgccgtgccc aacagcggcc aggccgtcat cattgatctc ggcgaagcca acgacatcca
     1801 ccccaaaaac aaacgcgacg ttgccgagcg cctcgcccgc atcgccctgg cgcgtgacta
     1861 cggcatggac atccgctggc gcagcccgga gttcaaagcc gccgagttca aggaaggcaa
     1921 agccatcgtc accctcgaca ccttcggatc gagcctgcgc accgtggacg tgagcgaggt
     1981 caaaggcttc gccctctgcg gagaggaccg caagtgggtc tgggccgagg caaagatcgc
     2041 cggcagggac aaagtggagg tcagcgccaa gaccgtgccg aaccccgtgg ccgtgcgtta
     2101 tgcgtgggcg gacaacccgg tgtgcaatct tttcagcatc gagggcctgc ccgtcacgcc
     2161 cttccgcagt gacgacttcc cgatgattac cgcgccgaag tgagcggcga agccaggcgc
     2221 ggcggaattt gtcgaaaatt caacatggcg gggccaaatt ccgccttgcc agccagacgg
     2281 atgccattac tgtccgcccc ccttcccgcc tgggttataa ttccaggcgg gcccgtcacg
     2341 ccgatgaaag ccgaactcgt tgaacaagcc ctgcatatcg tcaaagaccc gcccatcctg
     2401 atcaacatgg tctcgaagcg ggtgaagcag ttgaccaccg gacgcgcgcc tctggtggag
     2461 cgccgggccg gcctgcgcga ggcggactac gccctgctgg agatcattca gggcaaaatc
     2521 accaccaccg acctggagga gtgatccccg gccaggcggc ccgaaccccg ccgccccatg
     2581 tctgaagaga tcgccaccaa ccggaaagca ctccgggatt accacatcct cgaccgatat
     2641 gaagcgggcg tggagctgcg cggcacggag gtcaaatcca tccgccaggg aaggctgaac
     2701 attaccgacg ccttcgcgcg ggtggagcgc ggccaggtgt ggctgtacaa catggacatc
     2761 cagccctatg acaaggccag ctttgagcag cacgagccgc gccgcgcgcg caggctgctg
     2821 ctgcacaagc gggagatttt gaaactcttc gctcacaccg agcagaaggg gctgacgctg
     2881 ccggtgctca gagcgtattg gaaaggctcg cgcatcaaag tggagctggg cgtgggcaaa
     2941 ggcaaaacca agggcgacca gcgcattgac ctcaaggaga aagccgtgcg gcgcgagacc
     3001 cagcgcgtgg tctccagctt caaccgcaag ggaaagcagg gttgactgag atcagcccgg
     3061 cacgagtccg ccgtccttga ggcgcaggag ccggtcgccg cgtttcgcca gggccaggtc
     3121 atgggtgacg acgacgagcg tcttgcctgc ctcgccgaca acgcccatga gcatgtccat
     3181 gacaccggcg ccggtgccgg cgtcgagatt gccggtgggt tcgtcggcgt agaggatgcc
     3241 gggatcgttg atgagtgcgc gggctatggc gacgcgctgc tgctcgccgc cgctgagctc
     3301 ggtgggcaga tggtccatgc gtccggccag accgacacga tcaagcagtt ccctggccct
     3361 gtccccggcc ttgcgcccgc cgatcatggc gggcagcagc acattttcca aggcggtgag
     3421 ctccggcagc aggaaatagt gctgaaagac gaagcccatg gtctggttgc gcgccctggc
     3481 gcgcgcggcg gcggggccgt ggtagaggga tttgccgtgg acgagcacgt ctccgcgggt
     3541 gggtttctcc aggccgccga ggacataaag cagcgttgtc ttgccggcgc cggactggcc
     3601 gcagaggaag actttctccc cggcggccac ctcgagttcc acgccacgca gcactctcaa
     3661 ctcatgcccg ccgagcacat aggtgcggtg aacgtcctgg gcgatgagcg gggaggatga
     3721 ggcgggcatg gcggggagga agggcgggca atctctgacc gcgctcagtg ccgcgcgtca
     3781 aacgccattt ttcccagcgc cagcgcgccg agcaggccgg agcgcgcgcc aagcccgggc
     3841 gggacgacga actcctcaag atccctgcca aattggggca ctgagaggta gccgttcata
     3901 tgctggcgca gcttgcccct gatcagcggg acgagatgcg gctggctcat gacgccgccg
     3961 cccagaatga tgcgccgggg acaaagagcc agtgtcagat tcatcaaggc gtggcccatg
     4021 gtgcaagcca cctcctccca ggcggggtgg tcggcaggca gcgagtcggc tctcactccc
     4081 cagcggtgcg ccagcgcggg accgctgacg tagccttcga cacagtgctt gtgaaacggg
     4141 cagaagccct cccgctgcac agccaggctg ttttgcggag gcggaaccat gagatggccg
     4201 atctccgggt gcatgagtcc gtgcagcagg cgtccatgca caagcacgcc tccgcccacg
     4261 ccggtgccaa cagtgaggta aacgagcggg tccatcccct gccccgcgcc ccagagatac
     4321 tcgccgagca cggcggcgtt cacatccgtg tcgaagccga cgggaacctt gaagcggttt
     4381 ttgaaaaagt tcaccaggtc agtgttctgc cagccgggtt tcggagtggt ggtgatgctg
     4441 ccgtaggtct cgctggcagg attcaagtcc accggaccga aggatcccac gccgatggcc
     4501 gccagggggc cgtgcgccac cttcattttc gacagccagt ggctcacagc ctccagggtc
     4561 tcggcaggcg tggtcgtggg aatgcggatg acatccagaa gctggtgcgg atcacgaccg
     4621 atgccgcaga cgaacttggt tccgcccgcc tcgatggcgg cgatgagcgg cgcttcctgg
     4681 ctcatggctt gcgcacgtcc gccgagtcgc ggatgccgag gctggcgtag atttcgcgcg
     4741 tggcctggga tttgttgagg gtgtagaaat ggatgccgtc cacgccgtgc ccgagcaggt
     4801 ccaggcattg ctcggtggcg taatgcacgc ccacgcgccg cacagcctcc tcgtcattgc
     4861 cgcagcgctg gatggcgcgc agcagtttgg cagggtaacg cgccccggcg gccagctccg
     4921 ccatgcgcct catgcctgcg gcgctggtga tgggcatgat gccggcgatg atggggatgt
     4981 ggacgcccgc gagacggcag cggtcgcgga agtcgaggaa gtcgtgattg tcgaaaaaca
     5041 actgcgtgca gatgtaatcg gcgccctcgt cgcatttggc tttcagatgc tccatctcca
     5101 gcacgcggtt cggcgtggcg ggatgtccct ccgggaaacc ggccacgcca atgccgaagc
     5161 cgcgcctgtc tggatgcgct ccactgtcgc tgaatttttt gatgaagcgc acgaggtctg
     5221 ccgcgtgctg gaaggcgtcg ttttttcttt catagccttc caggttgcgc ggcgggtcgc
     5281 ctccgagcgc caggatgttg ctcacgcccg cctgcgcata gcgctccagg atggcctgga
     5341 tgtcgccctc gctgtggcag acgcaggtga gatgcggcac gggatcgagc ctggtggttt
     5401 tttgaatgcg caccaccagg tcgtgagtca gctcgcgcgt ggatccgccc gcgccgtaag
     5461 tgacgctgac aaaggagggc tgcaatgcct ccagctcgcc gatggtctgg tagagcgcct
     5521 cggacgcgtc cgccgtcttg ggcgggaaga actcaaaaga aagcgtcggc ttttgctggg
     5581 aaagaatatc ggcgatgtgc attcgtctgc gggtggcgaa tagaacccgg acaccttcga
     5641 acggcaagca cggaaagctc acccgcccgc cagcagcagg gccgcccatt ccccggcccg
     5701 ccaggcggcc aatgatgcca gcaggatggc gatgacgacg cccaaagcgc cagtcagtag
     5761 aatttccagc gccctggagg ccagcagcga ggagcgtgga acaccgatgt cctccagcgt
     5821 ccggaactcc cgccgccgca gccggaagga cagagcgaaa accagcgccg cagccgccag
     5881 cgcgcccgcc gagacaaggc cgagcaccat caggacaagc cgctccagcc tgaacagcgt
     5941 gtccatcagc gactggaact catccagcgg gcggatcaac tggacgggca gcctttcatc
     6001 caggtaacgc ccggccagga tggcctccgc cttcttgccg cgcggcagca ccaccgccgc
     6061 gctcaccgga aacgaatccg gatcgccatg aaaatggaag ctgccaaggt tttcgtccgt
     6121 cacctcttgg aaagtgcgca acgctgcgtt cgcaaccgtg ttgcctgcct cctcccgcag
     6181 tgtgtcctcc tccgccagat cgtcatggcc gtgcgccagc ccctcgatga gccacgcggt
     6241 tttgatgtcg gcaaacacag ccccgtcatc cgccgtgccc gaaagagcca gcacacccgt
     6301 cacccgcatt tttagaggat gactccccgc cagatcaaaa acctgctcct gcgaggtaaa
     6361 cacattcccg ccaggcttca aaccgcgctc agccgccaca cgcgcgccca ccacgcaatc
     6421 ccccagccgc gcaaaccagc gcccgccgtc caaccgaagc ccccggaact cgaagtaatc
     6481 cagctccgtg cccacgacgg gggcgccctg gcaatgaaaa cgcgcgtgca gcgggatcac
     6541 gcttccctgc cccgcgtcgc gcgcctcccg caccacgcgc atctcgatgc ggggcagcgg
     6601 ctgccttttg aaataaagcg cggtgaacag aagatcgagc gcgctgccgc gcgcccccag
     6661 cacctgcgga gtcagctccg cacgcgaacg catttgatcc ccggcggcgg acagcagaac
     6721 gcgcagcgaa aacggcaccg ccatcatgag acccagcact cccgccagca gccaggtctg
     6781 ggcgcgctgg caggccacat gcaggaaagc cagatgcagc gccgcgctca tgcccgcgca
     6841 tcccccaggt tcagattcgc cgggaagatc gccgccgccc gcgcgtcatg ggtcgccatc
     6901 accagcacac cgccgctttc ccggcaggtc cgcaacagca ggtctgtcac cagtccgcag
     6961 cgcccgctgt ccagtgaagc cgtcggctcg tcagcgaaaa ccaaatccgg cccgtgcgcc
     7021 agcgcgcgcg ccacagccac ccgctggcgc tcgccctggg aaaggcgcgc ggctggccga
     7081 tcccaaaaag aagccacctc cagacgctcc gccaattcac gcgcccgctc ccgcgccccg
     7141 gtcatgcaaa ggcccaaaaa ccgcgcgggc agcagaagat tttccgccac cgtgagatga
     7201 tccagcaaag cgaagtcctg aaacaccagc cccatccggc gaagtcgaag cgcgcgcgtc
     7261 tgcgcgggag tcatcgcgcg caaatcctcc ccgtccacac acacccgccc cgctccggga
     7321 ctcagaagtc ccgcgagcag gcgcagcaga gtggacttgc cggagccgct cggccccaga
     7381 acagccaaag ccttccccgg acccgcctcc acactttcca agcagagctg gaatccgccg
     7441 ccgggatgac ggaagcgcaa attttcagcg gaaagtgaag ccatgacgac agcggctccc
     7501 cggccggact gcggacaacc cggtcaagcc agcggcgcgc cgaggcggcg cagcatggct
     7561 tcgagcatgg tgccgtattt gatgtagctg tcgtattgca gcctggcctt ctcgtcggga
     7621 gagagatcat ccgccgcgcc ggcgccgtgg aagacgactg ccacatgcgg ctcgatgctg
     7681 atgcggccgt tgtaaccgct ctcgacaagg tctttcatga tgcgctcggt ctggccgtcg
     7741 ccctcgccgg gaaaggtgta ttcggcatcg cccttttcct tgatccagcg accgtctttg
     7801 acgtgaacat ggaccatgtt cggcttgacg gcctggtaca tctcccagga gtcctgcttg
     7861 tggccgggac ggtcgcgatc gtcaatgaag acggggttgc cggtgtcgaa gacccatttc
     7921 atctcgggca cggcctccat catgcggcgc acataggcgg ggctcatgcc gccccagttc
     7981 atgcagttct catgcacgac ggtcagcccg gcatcagcga agcgcttgtt gatctccctc
     8041 atccggcgca cgcgctcgtc cacatgctgc tcggcggcgt cgctgccgtc gtcgtttttg
     8101 cagacggcgt agctcatgac gcggatgagc ttggtgccca agcgctgcat gcgcgggatg
     8161 gcgcggttga tctcggcctc ggtgatgctg aagtcatcgg tgattttttt ggcccagttg
     8221 ccaatgaggg agccgaagcc gctgacgccc atgccggctt gggctagctt ttcgcagatt
     8281 ttgtcaaagg cggcctcggg cacctcgtgg aggttgcctt tgacgccgtc cagttcgacg
     8341 acacgcattt cgatgctgtc ccagccgagc tgtttgtggg cgcggatttg cacatccagg
     8401 gaggctcctg cctcgtcggc gatgccggtg agtttgatca tgtcgggttc agggttggag
     8461 tttggggaaa agaggggtca ggcggcggcg ctggtgttga aaggacgaac cgcgaggatt
     8521 ttcaccggct tgggagcgcc tgattcggag gggatttcgg ccgtgtctcc gggtgccttg
     8581 ccgatgaagg ctttggcgat ctcggagaga taagagacga tgttgttctc cacatcgcca
     8641 tcccaagcgc ccaggatggt gagagtctcg gtccgaccgc cgccgggcgc ctcgtaatcc
     8701 acgatggtgc caatgccggc cttgtccgtg gcggcaccgg cgaaatcggt gccgcgggcg
     8761 aggcgcagtt cgcgctccat tttgttttgc aggcgcagaa ggacggactg ctgctccttg
     8821 gcggctttgt aaccgccgtt ttcgcgcagg tctccctcgg cgcgggcgat ttggatgtcg
     8881 tgcttgttct gggggatttt gacggtgacc agttcctcga gttctttctt tttcctctcc
     8941 aggctctccc aggagacaat gaggctgttg tcctcgcgcg ggccggcatt ttcggccatg
     9001 agcacctcca tttcgggccg cgccttgatg atgcgcgcca tgagggagcg gcgggtcagc
     9061 tcgtcaaaaa cggaggtgtt aatgatgcgc ttggcaaagc ggcgcacctc gtcctcctcc
     9121 gtctcaatga ccagctcgtg gatgagggtt ttgtcgtcgg agagcaaatc gctgagacgg
     9181 cccgtgcgtt tggggccgcc ctggacgtgg tcgtgctcga tggcgtcgag gatggcgttg
     9241 gccaggtcaa tgtcgaagac ggactcggcc aggcccttgc gctccttgca gatccagatg
     9301 agcaggtcgg tggtgagggc gcggttgcgg acggcttttt tcagaaactc ggccagcacg
     9361 tcgagctcgt cgttggcatc aagcacggtg gcgatctcgg ccacggcgcg gccgccggtg
     9421 cgggtgaggt ggttgagtat ttccggcacc caggcgcgtt cggggaaggc gtccgggaag
     9481 gcgcggtaca tgcggcccag caggctgaca ggcagtccgc ccacagcctc ggtgagctgg
     9541 gctcgcgcct cgctgatgag gtcggcgatt ttcatcgaac ccagcgggag cgcggcaccc
     9601 tggatggact cgatgacctc gtcccgcagc agaatgatct gcaggctgtc cttcaaatgc
     9661 aggcgccagg ctttgcggac cgtttcggaa atgtcctgaa gcgcgggcag cagttccctg
     9721 gccgggtctt cgaagaggtc gaggtctttc tggatgagag ccagggcggc cagtttgccc
     9781 ttcatgtcgc gggcgcccag gtaggtgcgt atcatgccgt cgccggcctt ttcctcggaa
     9841 ctgcgcagga tgagaggttc cgtgcgtttg gcgggcacca cgatgtggcg cgcatttttg
     9901 agcgtcttct tcgccccctc ccaccatttt ttatactcgc cctccgtcaa cacatgcggc
     9961 ttgaggaggt tttccagctc gcccaggagc atcctgcccc ccttcgcgga cttgagggcg
    10021 atctcgacga gagcgctcgc attttccctg gccatctgct tgaggccttc gaggtcggcc
    10081 agcctgcgcg ccaggaagtg atcctggggc agcacctcca gcgaggtgat ggcgaaggaa
    10141 attttcaggc cgtgccccgg cttgccctcg aaatcgagga tgattttgtc ccccagcaga
    10201 tcccactcgg ccaccttgcc cactccccag cttttgtgcc ggcaaaaggt gccgggttcc
    10261 aactgggaga gtttttcgcc gtctgcggct gccagcttgc cggcagcgac caatttttga
    10321 gcctctgaat tcatgggaat ccgatcataa agcgcgcgcc gaaggtggca aggactcctt
    10381 gtcctggaat tcccgcgccc cggcgaaaaa tccccccttg tgaaaaaaaa cgagaatctt
    10441 tcttgccagg aaggccgcgc gtttgctatt tctcaataac ccgcatgaaa aatcgcatcg
    10501 cactgacaat cctctccggc ctgacgctct gcggcatggc ccttgccgac atccaatccc
    10561 ctcccggaca ccactataac tggagccgca agctgagccg tggtgtgggc aacctcctct
    10621 atggatggac ggagcctttc actgtctggc agcgtgtgaa ccgcgctgaa ggcgcccatg
    10681 ccgccgcgtc cgacttcttc attgagggct tcaagcgcac cgttgtccgc ctcggctacg
    10741 gcctctacga ggtggccacc ttccctcagc ccacctggaa atgcacctac cgcccgcctt
    10801 attacaagaa ggaagttctc gatccatggt ggggctacac ggagttttct ccgcaaatcg
    10861 gtttcgcctc ccacatggat tacacccggg tgcagggttg gtgatcgaac accagaattc
    10921 acaaagcgcg gacccaggtc cgcgcttttt ttgcgcccgt tcattccgcc gcgcgcaatt
    10981 tccgccggat ttgcgccagc acatagcggc aggcgagcag cgaggccgcc atctgcacaa
    11041 gcacggtcga aagcgcgatg cccgccacgc ccatgcgcca catcagcagc cagttcagcg
    11101 ccgcgttccc cgccaggccg aggaatgaca gcatgaggac gaagcgcgtg gcctgcatcg
    11161 ccactaccac gcgcgaggcc aggccgccca ggatgtaaaa ggggatttgc agcgccgcgt
    11221 agcgcagcac cccgcccacg cgccgcgtgt catcctccgt gaacgagccg cgctcaaaga
    11281 gcagacccac cacccacggc cccagccagc agagcagcag ggcggcaggc aaggccaggg
    11341 aaatgatcag gcccgcgctg gccagcaact ggcggcgcac cccctcccag tcctggcgcg
    11401 ccaccttgtc cgcgaaatag gggaaaagca catccgccgc cggagccgcc gtcaccgcca
    11461 ggatgatgga acagaccttt tccgaatagc ccagcaccgc cacgctgccc ggctccagcc
    11521 aggaggccat gatctggtcc accaccaccg cgctgctgaa aaccaccccc gccagcagat
    11581 aatgcgccgt gctcctgcgc acctggcgca ggccggggtc gggcagaaaa gcgcgcctca
    11641 tccccaggcg caaaccgccc gcggtgcagg ccgcgagcac catcaggtgc agcgctgccc
    11701 ccaggcaggt gccatgcacc agcgtccaca catccgcctc cgctccctgc gccagcagca
    11761 ggacaagcat cgtcagaggc accagcatgg gcgcgctcgt cgccgcggcg aatcgcttct
    11821 ccgcgcgcag ccaggctgcg agttggaagc tcatcccgaa gcagagcaaa aacggcagca
    11881 gcccgcgaag catcgccacc gccagctcct gcttctccgg cgggaagccg cgcgccgcgc
    11941 gcagaatcag ccccggcgcg gccagccagg ccgcgccagt catcaccagc aggatgccgc
    12001 catgcagcac cgcgctctgc accgcgaatc tctgcgcccg gcggaagccc gtcctgttcc
    12061 tgagtccggc gtagacgggc aaaaacgcct ccggcagcgc tccgccggcc agggaacagg
    12121 cgaaggccag cagaccgaaa gccatcaaaa aagcgtccag ctcatccccc gtgccgaaga
    12181 agcgcgccac cgccgcgtcc ttcaaaaacg ccgccacctt cgccacgatc gtcagcaccg
    12241 ccaccagcag aaagcccgac accatgcgcg aacccgcgcc ccgtcccgcc agggcgcgca
    12301 gggagctgag aatggagccg cgcccgttgt gcaaagtgcg gtaccgtatg ccctgcgcgc
    12361 ctgccggcaa ggattccggg gagatttgtt caagcccccc tgccctgccg tcacaccttc
    12421 cgccagcgcc tggccttgaa ttcatgcctc ggcaggctgc cgggcagggc cagggacgcc
    12481 ggtatgcgca gggagaaagt gtcgcgcagc ttgttctcca ggcgtttaag cagggacttc
    12541 gacaccgcct cgggcgcctc caccagcagt tcgatctcgt ccatgtcatc cactttgcgc
    12601 tgctccacca tgaactcggc cacctccggg aactgccgca ccaccgcctc caccgcgctg
    12661 gggtagatgt tcaccccgcg caccaccacc atctcgtcca gccgccccag cacgccgccc
    12721 tccagcatga gccggttgtt gaccatgcgc ttgcgcaccc agtcgccggt gcggtagcgc
    12781 agcagcgggc aggccgcgcg gtccagcgtg ctcagcacca gctccccctg cgtcccctcc
    12841 tccacctccc gccccgtcgc cgggtcaatg atctccgcca gataagcgtc ctcgatcacc
    12901 tgcaggcaca gcggcagatc agccgactca aaagtcacag gccccacctc cgtcatgccg
    12961 tggtggtcat gcacggacgc atcccacagc ttttcgatgc gcgcgcgcac ctccggaatg
    13021 ctgccgcccg gctcgccggc cacgatgatt ttcgtcacgc gcagcgccat gcgctccacg
    13081 ccgctggcct cgccgatcat ctcgccgagg cgcaaggcgt aggtcggcgt gcagcacagc
    13141 accgtggcgc cgtagcgggc catcatctcc agccgcgcct ggctggaaag cccgccgccc
    13201 ggcagcgcca ggtaatcccg cgccgcggcc tcgaacgccg tccagaagcc caggaaaggt
    13261 ccgaacgaga aagcgaagaa aatgcggtcc ctgcccttcg ccagccccgc cgcctcatag
    13321 accctgcgcc agcattccag catggcctcc cagctttcct gcgtgtccag ccaggccatc
    13381 ggctggccgc tgctggtgcc gctggtctgg cagaagcgcg tgtactgcgc cggatgccgc
    13441 gtcagattcg tgccgaaggg cggatgctcc agccggtccg ccagaagctc ctcctttgtg
    13501 gtgaaaggtg tctgcgccac aaactcctcc agccgcccca tcgaggtcac gcgcacgcct
    13561 gcctcccgga atttgcgcgc gtaaaagccc tccgtcgtcg ccaggtgcat cagcaggccg
    13621 cgcagcttgc gccattggtt ggaacgcaag cgcgcacggt ctatcagggc ggattcatgc
    13681 ggcacatggc cagacttggc gagcgcgcca gcggggtcaa ccctgtttgc gcggcaacgc
    13741 gccgtcccgc gccggtcacg ccaccgagcc cgccagctcc gccatcgccg cgcccgcgtc
    13801 cgccgggccg aaaaacgagg tgccgcagac aaacaagttc gccccgtgcg ccttcgccac
    13861 cggcgcggtg tgcgcgtaaa tgccgccgtc cacctcaaga tgatagctca atccatgggc
    13921 ggcccggtaa tcgcgcgcct gcgccagctt cggcatcgtc tccttttcca tgaatggctg
    13981 cccgccgaaa cccggcacca ccgtcatcac cagcagcagg tctatttccg cgatgaacgg
    14041 cagcgccgcc tcgaaaggcg tgtccgggtg cagggccagg ccgcagtgcc tgcccgccgc
    14101 gcggatgcgc cgcagcgtct cggccacaga ggtctcgtag cgcgcctcgg cgtggatcgt
    14161 gatgttgtcc gcgccggcct gcaaaaaccg ctccagataa tgatccgcgc ggtggatcat
    14221 cagatgcaca tccagataca tgcccgtcag cccgcgcacc gtccgcacca tttgcggccc
    14281 gaaggagatg ttgtccacaa aggccccgtc catcacatcc agatgcagcc agcgcgcgcc
    14341 ggcgctttcc gctcgcccga cttcggcgcc tatccgcgac cagtcggcgg cgagcagcga
    14401 ggggaggatc aggggtgtct catgggtcat gacaggccat caagcacgcg ccccgccccc
    14461 atgcaagggc ggggcgaaaa tgacggatgg atttcaatgg cacaacgccg caaaaccaga
    14521 aacaaacccg aatgccttcc acacaaaaac ccgccgggac ccatgcccgg ccgaccttgg
    14581 aaaaggcaat gggggactca cgaaaacacg cttttgcctg tgccccatgc tctgagtttt
    14641 cggcattcaa ccgcttgccc tgttgggcgg ctggcgcgcg ccggactgcc tgcccggtgc
    14701 ggttgcggaa agattgcctg gggctggcgg cggcgattcg ctcgcgcggg catcattcct
    14761 gcttatctag gcgggatatg acgccaaccg aaccgctgcg ggagcatggc ttccgatttc
    14821 ttgaggatga actccgcttc atcatggacg cttttgccaa tgtgctgcgc cggctgggaa
    14881 accccggcct ggcctcgcgc ctgccgtggg cggggcagtg cgggg
//
LOCUS       DAOMYJ010000122        14917 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Prosthecobacter sp. isolate AMR_MDS_5629
            NODE_1366_length_14917_cov_18.232136, whole genome shotgun
            sequence.
ACCESSION   DAOMYJ010000122 DAOMYJ010000000
VERSION     DAOMYJ010000122.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867850
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prosthecobacter sp. (wastewater metagenome)
  ORGANISM  Prosthecobacter sp.
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Verrucomicrobiaceae; Prosthecobacter.
REFERENCE   1  (bases 1 to 14917)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14917)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.30
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:34:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,742
            CDSs (total)                      :: 4,684
            Genes (coding)                    :: 4,676
            CDSs (with protein)               :: 4,676
            Genes (RNA)                       :: 58
            tRNAs                             :: 55
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14917
                     /organism="Prosthecobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1366_length_14917_cov_18.232136"
                     /isolate="AMR_MDS_5629"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1965333"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(133..615)
                     /gene="coaD"
                     /locus_tag="PLP58_13240"
     CDS             complement(133..615)
                     /gene="coaD"
                     /locus_tag="PLP58_13240"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009962353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004595 - pantetheine-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HRJ09535.1"
                     /translation="MRRAIYPGSFDPITNGHLDVLHRAAGLFDELIAAVAHDNSKQSL
                     FTLEERVALLSDATAHIPNLRVLPFKGLLVDFARQQSAVALVRGLRAVSDFEFEFQLA
                     LMNRKLEPNLETLFLMPREELTYISSRLVKEIARLGGNVNQFVPPNVTAALKTKLDLP
                     "
     gene            772..1374
                     /gene="aat"
                     /locus_tag="PLP58_13245"
     CDS             772..1374
                     /gene="aat"
                     /locus_tag="PLP58_13245"
                     /EC_number="2.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012421000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008914 - leucyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl/phenylalanyl-tRNA--protein transferase"
                     /protein_id="HRJ09536.1"
                     /translation="MIDPAQLLSAYCEGAFPMGNEDGSLHWFRPRWRGILPVREFHVP
                     RRFLRDLKKSPFEVRWNTAFGEVMRGCAEREETWITDVILDSYQELHRIGMAHSAEVW
                     RDGELRGGVYGVAIGGAFFGESMFSREPQASKTALTHLQWRLKERGFILHDTQWTTPH
                     LAQFGGYEIPCAEYLELLHRAIRLPVTFDDRSAPFLVAAR"
     gene            complement(1387..2220)
                     /locus_tag="PLP58_13250"
     CDS             complement(1387..2220)
                     /locus_tag="PLP58_13250"
                     /inference="COORDINATES: protein motif:HMM:NF013247.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class IV"
                     /protein_id="HRJ09537.1"
                     /translation="MNKTPHIWLNGVILPAAEARISPFEHGLLVGDGVFETLVMLRGR
                     VIGGHEHWQRLERSCARMGLPCMTEAEFLSAIGEVARANGLADARVRVTLTSGDGPLG
                     SDRGAGRGTRLAAATPLNTWPPVEKVRLVPWTRNENGALAGVKSISYAENVLALAEAK
                     KHGCGEALLASTRGELCEGTGSNIFVVAGGALMTPPLSSGCLAGITRLLVLRACGRAG
                     IPCAEQAMPAAVLDTCDEAFLTSSTRHVHPIAEVNGRALAAPGPVTLAVRAAYSELIG
                     L"
     gene            2355..2942
                     /locus_tag="PLP58_13255"
     CDS             2355..2942
                     /locus_tag="PLP58_13255"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009962062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HRJ09538.1"
                     /translation="MLRFESFHGSALAPHLDALGALRISVFRDYPYLYDGSVDYEREY
                     LKCYLDCPESLVVLVFDGGQAVGATTAMPLAFEGPEFQAPFLKAGMDVAEICYFGESI
                     LLREWRGRGIGRTFFQMREAHARALGLRRAAFCAVDRPPEHPLRPADYRPLDGFWTSQ
                     GYTKHPELRATFVWKESGEQAESPKTLTFWLKELR"
     gene            3122..5476
                     /gene="pbpC"
                     /locus_tag="PLP58_13260"
     CDS             3122..5476
                     /gene="pbpC"
                     /locus_tag="PLP58_13260"
                     /inference="COORDINATES: protein motif:HMM:TIGR02073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009274 - peptidoglycan-based cell wall
                     [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 1C"
                     /protein_id="HRJ09539.1"
                     /translation="MERIAHRDGPRAGFRILPAAASAARKGLAVVVVCVSGYALLPRT
                     ELYPPEFRFSRALEDRDGRLLHLALTPDGKYRRCTPLAEISPALIEATLTLEDRHFYG
                     HPGVNPLSLLRAAWGAVSGSAKGGGSTITMQLARLRHGLRTRTPWGKAWQIVRAVQME
                     RHHSKDEILEAYLNQAPYGGNVEGAGAAALLWCGKTARELTLREAVTLAVLPQSPTTR
                     RPRMSGENASLAAAQHRLWERLRVERGLPADPLDAVFTLWQARPAPREAPHLARRLFT
                     LHEPHDRAASGAPVRCTLSVEKQRGVERALQDYVERKREVGITNACALLVHAPSREVL
                     AYAGSSRFLDPSIQGQVDGVTARRSPGSALKPFIYALALQEGLIHPRTLLRDGRTAFG
                     AYNPENFDRNFAGPISAQDALFHSRNIPAVALAQKLAPPGLYGFLKAAGVALPRPAGH
                     YGLALPLGGGEVSMEELAELYSLLADDGRARRLSLQPGGELEHQSRPLITAEARHLTR
                     MMLSAREADPAMDDPSVMWKTGTSHGFRDAWAAGIRGNYVLVVWIGNFNGKANPAFIA
                     RECAAPLLFEVFRHLRLPARADPPPPGVARVELCAVSGQLPVPHCRHRARGGFIPGVS
                     PITPCAIHREVLVDAASGLRVARDDGSRALRREVFEFWPPDLLEMFRQAGVPRRTPPP
                     LEFDSDSLAAADIAAPPKILSPRAALVYTLRASEPARQTIPLRADTAPGVARVFWFAG
                     PRFLGASDPATPLLWSASPGRWQVHVLDDHGRGAAVEARVEMVP"
     gene            5656..8880
                     /gene="secA"
                     /locus_tag="PLP58_13265"
     CDS             5656..8880
                     /gene="secA"
                     /locus_tag="PLP58_13265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009960958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HRJ09540.1"
                     /translation="MFNWIIKKIIGTKNQRTVRRLQPVVAEINRIEAELQNLPDEALR
                     EKCAAWKEQFRAFHTPRFLGGVSLRIASEEEVDACLRHVAGYFAALQKHFPSLAGDYL
                     AESSWNGSDLDDKKARIDRGREDWNEIQPRFAAIEAKMLDDLLPEVYAVVKNAARRLC
                     GQEHSVCDTPLKWNMVHFDVQLIGGAALHRGMIAEMATGEGKTLVGTLPVTLNALTGR
                     GVHVITVNDYLARRDSEWMGYLYKFLGLSVGCIQNDQPSHVRREQYACDITYGTNSEF
                     GFDYLRDNGMASSREQQVQRGHYFAIVDEVDSVLIDEARTPLIISGPVAGADSTHQYE
                     RYKPLVEQLVRRQQTLCNSLVTEAKELAAAGDLEEAGRKMYQVHVGQPKNRALMRCME
                     DPELRRAMEKAELKMYEDTQKKELFALKEDLFFSMDPKSHDADLSEKGRNFLNPNEPD
                     AFVLPDLATLYSEIDGDTSLTDAQKLAKKNELQDRLSHQGQRIHQISQLLRAYCIYEK
                     DVEYVVEENKVIIVDSQTGRKMPGRRWSDGLHQAVEAKEGVQIDAETQTLATITIQNY
                     FRLYQKLGGMTGTAETDAAEFHDIYGLDVLTIPTNRPVKRLDQNDSIYKTRREKYAAV
                     IAMIHERHAKGQPMLVGTASVEASETVSRMLKLQKIPHSVLNAKYHRQEAEIVARAGQ
                     KGAVTISTNMAGRGTDIKLGEGVPDLGGLFVLATERHESRRVDRQLRGRCARQGDPGE
                     SKFFLSFEDDLMRNFGAADRMTKIMERFGMKEGEELQHPWLNRSVETAQKRVEQRNYT
                     WRKRVLEYDDVMNQQREVIYERRNDVLNSNDPRLLINDAVKEGIEERLEEFLPKDAGN
                     DMEPDHAGLLHWVNTTFPIGLRELDDAFKALDYQAQCDWIREKVLHAYDAKVAGANPQ
                     ALQEIEKMILLNAIDRLWQEHLYALDALKEGISLRSYGQKDPLIEFKQEAFSIFSELM
                     RSINGEVLGNLFKSTQQLAAFEQFLAQLAMQQQQGGAAAGQPPQQRKPAGEPRPHDPA
                     KEGPKLILPSAAPAPQKPKYSAGRNDPCPCGSGKKFKQCCGRVA"
     gene            complement(9202..9354)
                     /locus_tag="PLP58_13270"
     CDS             complement(9202..9354)
                     /locus_tag="PLP58_13270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ09541.1"
                     /translation="MMRCQNHNSLKINLSLNFTPIDYIETFYNRSRKPASTQEKKRAC
                     TQTRHA"
     gene            9579..11189
                     /locus_tag="PLP58_13275"
     CDS             9579..11189
                     /locus_tag="PLP58_13275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ09542.1"
                     /translation="MSAPAALSEITLGSTSCSYLPAKSLKTTTQLSSVLASSPRIRFV
                     GKVGGVAFDAVAVPDSGLVIKSLSLDLDTARPDGSRLVLTVNGRPVKTRIHDWELLPT
                     IRYADSDSTSCFTLFGELQDEEEGRRLRGDGARILNYDDAFQDSLMGLRMFQLDILLL
                     GDDFATDLPKEDGEYILGAGEKSPDLSTAKSLHEKYEEARLELDLKGIREDFDTEFQS
                     YVITDQSVDIRFVIKDGQLEITGEPFYYFWKDDEDDLNGRYQAAQAEEAVKAGVLKPG
                     DGFDELRAMIIKERKADQERKDSVKLKVARELKVISDTGTLEDLEQMETEADEKYEAA
                     LNKAVYEIAKAEGKVREGETPESFAERIFEEYMEADASVENAILERNQNWRNKAAENI
                     PEKKAEHAAFKKQMDDINVQVAGINAGPDAAYAALEDKLDEIRDKARQTVSEFQDSDE
                     ATGKYLKEFSAAHAQFVDTLAAMNPAVWNAGRTVMRYGAFFRYCRAHHSDAWSAFMQK
                     LGGNEGKLETKPAVKTPTVMKFSPPAGQ"
     gene            11197..13359
                     /locus_tag="PLP58_13280"
     CDS             11197..13359
                     /locus_tag="PLP58_13280"
                     /inference="COORDINATES: protein motif:HMM:NF025056.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003953 - NAD+ nucleosidase activity
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="toll/interleukin-1 receptor domain-containing
                     protein"
                     /protein_id="HRJ09543.1"
                     /translation="MSAESRTEPLVFVSFASGDDEAREELGRELHRQGIKAYDYSIPH
                     EGDQVGLEIVSQLSEKIEQAPFFIALVSSASTNPVKHIVHGEVRHAFRRTSLPEKNRC
                     VCVVLRGRRGDGSSYDPPNYWKLALAALNNYNASFPDFAIKVRGLNKTDPNCHGVVCF
                     RSRGTVADFDFSGTLEDKEAWPAVCHEAELYFEDGTWNSLLAALPPTSSFALGSLARK
                     NAWMGIYSHVQGRAWPHVDNVAPGCFNRVVMTVCNRLGVSYTRPVSPDSRLPFYDQLK
                     KEVAAMPLNQSISVLGQLDYFESRIMVGSWHDALVHIRSLLALLRQNNARSRFAEIIE
                     GVCLLRLGRSKDALSIYEGLMDRPDENICIMRAKALTAEGRHAEALAAARRAVEICDA
                     KHDPAALAHQEWLTLAVGSQNRAGAESFLADDAAWLREVSLANARAPHIFRSAPENLK
                     TEDRLKLRCLEAHWLKLAGRRSEALTKLENLLAEPVVSEVVRLAAGDLEGLPDQLTNR
                     FYARLADAGIYLDSPLEERIGEDGMTRITANGNREEFWLREQDRVYFFKQQQPWHYTI
                     RYLDKGTAMLRYELRLLPRREDDMALWHAALLELEDTLECLQDLHLCRHVIREWAEFG
                     GLHAAWRVFLRSRLWFPDDPYLLNDGAAIAAAAEDGEALEHLASEAERAARARFARCQ
                     PAGTEWTPAELLQAWAESQNLLGRDALSAAVHRFLNDA"
     gene            13510..13821
                     /locus_tag="PLP58_13285"
     CDS             13510..13821
                     /locus_tag="PLP58_13285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ09544.1"
                     /translation="MFLEHEAELLVLLQFPHFGPDGLLQPFVRDLSDEIFDLRHVSAP
                     IPAEDFFRWRNFFLDAGGGIPLFFSVNSSHEQEFAFGLPDGFQPPAPAYGSTASGQER
                     R"
BASE COUNT         2801 a         4666 c         4727 g         2723 t
ORIGIN      
        1 gcaggtcgta ggccagcggc gaggcggcct gcacactgtc accgggagcg aggtcaaaaa
       61 acgcgggagg cagggtgccg gcgacatggc ggccctcggc ggggagggtg cgcagttcga
      121 tgtgaaaagg gttcatggca ggtcgagctt ggttttcagg gcggcggtga cattcggcgg
      181 cacgaactgg ttcacattgc cgcccaggcg ggcgatttcc ttgacgaggc ggctgctgat
      241 gtaggtcagc tcctcgcgcg gcatgagaaa gagagtctcc aggtttggct ccagcttgcg
      301 gttcatgagc gcgagctgga actcgaattc gaaatcgctc accgcgcgca ggccgcgcac
      361 cagcgccacg gcggactgct gccgggcgaa atccaccagc aaccccttga agggcagcac
      421 gcgcaggttg gggatgtgcg cggtggcgtc ggagagcagg gccacccgct cttcgagggt
      481 gaagaggctt tgtttggaat tgtcatgagc cacggcggcg atgagttcgt caaaaagccc
      541 cgccgcgcgg tggagcacgt cgaggtgtcc gttggtgatc gggtcgaaac tgcctggata
      601 aatggcgcgg cgcatgacgg cagtttagcg gggcattcag tggcggcaag ggcgcaaggt
      661 gcggcgctat tggtgtgatg cgctccatgc cgcgggtctt tgccgccctg ctcggagatg
      721 attgcaggca gggcgttgca aagacagtcc gccatcgctt tattccccgc catgattgac
      781 cccgcccaac tgctcagcgc ctattgcgaa ggcgcgtttc ccatgggcaa tgaggacggc
      841 tccctgcact ggttccgccc gcgctggcgc ggaatcctgc ctgtgcggga gtttcatgtg
      901 ccgaggcgct ttttgcggga cttgaaaaaa agccccttcg aggtgcgctg gaacaccgcg
      961 tttggcgagg tcatgcgcgg ctgcgcggaa cgggaggaga cctggatcac cgatgtcatc
     1021 ctggacagct accaggagct gcaccggatc ggcatggctc acagcgccga ggtctggcgt
     1081 gatggcgagc tgcgcggcgg cgtttatggc gtggccatcg gcggggcgtt tttcggcgaa
     1141 agcatgttca gccgcgagcc gcaggcgtcg aagaccgcgc tcacgcatct gcaatggcgg
     1201 ctcaaggagc gcggcttcat cctccatgac acgcagtgga ccacgccgca tctggcgcag
     1261 ttcggcggtt atgagatccc gtgcgcggag tatctggaac tgctgcaccg cgccatccgg
     1321 ctgccggtca cttttgacga tcggtccgcg ccttttctgg tggcggcgcg ctgagcctga
     1381 aaagcatcac aacccgatga gttccgagta agccgcccgc accgccagcg tcacaggtcc
     1441 gggcgcggcc agcgcgcgtc cgttcacctc cgcgatggga tgcacatggc gcgtcgagga
     1501 ggtcaggaaa gcctcgtcgc aagtgtcgag cacggcggcc ggcatggcct gctccgcgca
     1561 ggggatgccc gcgcgcccgc aggcgcgcag gaccagcagg cgcgtgatgc ccgccaggca
     1621 gccggaggaa agcggcggcg tcatcagagc gccgcccgcc accacaaaga tgttcgaccc
     1681 ggtgccctcg cacagttcgc cgcgagtact ggccagcagc gcctcgccgc agccgtgttt
     1741 ttttgcctcg gccagggcca gcacgttctc ggcgtaggag atgcttttca ctccggccag
     1801 cgctccgttt tcatttcgcg tccagggcac gaggcggacc ttttccaccg gcggccaggt
     1861 gttcagcggc gtggcggcgg ccagccgggt gccgcggccg gccccgcggt ccgagccgag
     1921 cgggccgtct ccactggtga gcgtgacgcg cacgcgggcg tcggccaggc cgttggcgcg
     1981 cgccacttcc ccgatcgcgc ttagaaactc cgcctccgtc atgcagggca gccccatgcg
     2041 cgcgcaggag cgctccagcc tctgccagtg ctcatgcccg ccgatgacac gcccgcgcag
     2101 catgaccagt gtctcgaaga ctccgtcacc caccagcaat ccatgctcga atggcgaaat
     2161 gcgcgcctcc gccgccggga ggatgacgcc gttgagccag atgtgcggtg ttttattcat
     2221 ggcagtttat tcatggaccg ggcgcgtttc ttttgcatcc gggtttttgc gcggggcggc
     2281 aaagactgcc cgttttgaaa ttcgaacctc gaatttcgca agacatcgga tgggtgtggt
     2341 ggcaaagcag cgccatgctc cgcttcgaat cctttcacgg ctccgcgctg gctccgcatc
     2401 tcgacgccct cggcgcgctg cgcatctccg tttttcgcga ctacccttat ctttatgacg
     2461 gttctgtgga ctatgagcgc gagtatttga aatgctacct ggactgcccg gaaagcctcg
     2521 tcgtgctcgt cttcgacgga gggcaggccg tcggcgccac cacagccatg ccgctggcgt
     2581 ttgaaggtcc ggagttccag gcgcctttcc tcaaggccgg aatggatgtg gcggagatct
     2641 gttattttgg cgaaagcatc ctgctgcgcg aatggcgcgg gcgcggcatt ggcaggacct
     2701 tctttcaaat gcgcgaggcc cacgcccgcg ccctggggct gcgccgcgcc gcattttgcg
     2761 ccgtggaccg cccgccggaa catcccttgc gtccggcgga ctatcgtcca ttggacggat
     2821 tctggacaag ccagggttac acgaagcatc cggaactgcg cgccaccttt gtgtggaagg
     2881 aaagcggtga gcaggccgag tcgccaaaaa cgctgacctt ctggctcaag gagctgcgtt
     2941 gacatgccgg cgctccatgc gccggccttc cgctggatgc gctcatgccg gagcattgtg
     3001 ccgcgcaagt ccaggcggca tcctggctca ggcaggcggt aggaggatcg ttttccccga
     3061 atctgcggag ttgtgtgaaa accgcttgtc atttccccgc caggatcaca ctccagtccg
     3121 catggaacgc attgcccatc gtgacggccc gcgcgctgga ttccgcattc tgccggcggc
     3181 ggcctccgcg gcgcggaaag gccttgccgt ggttgtggtg tgcgtgtcgg gttacgcgct
     3241 gctgccgcgg acggagcttt acccgcctga gttccggttt tcacgcgcat tggaggacag
     3301 ggacggccgg ctcctgcatc tggcgctgac gccggatggc aaataccggc gctgcacgcc
     3361 tttggcggag atcagcccgg cattgatcga agccacgctg acactggagg accggcactt
     3421 ttacgggcat ccgggagtca acccgctctc gctgctgcgc gcggcctggg gggcggtgtc
     3481 aggctcggca aagggcggcg gctcgacgat cacgatgcag ctcgcgcggc tgcggcacgg
     3541 tctgcgcacc cgcacgccct ggggcaaggc ctggcagatc gtgcgcgcgg tgcaaatgga
     3601 gcgccatcat tccaaggatg aaatcctgga agcctacctg aaccaggctc cctacggcgg
     3661 caatgtggag ggcgcgggag cggccgccct gctttggtgc ggcaagaccg cgcgcgagct
     3721 gactctgcgc gaggccgtga ccctggccgt gctgccgcag agtcccacca cgcgccgccc
     3781 gcgcatgtcg ggtgaaaatg ccagcctcgc ggccgcgcag caccggctct gggagcgcct
     3841 gcgcgtggag cgcggactgc ccgccgaccc gctcgacgcg gtcttcactc tctggcaggc
     3901 acggcccgcg ccgcgggagg cgccgcatct ggctcggcgg cttttcaccc tgcacgagcc
     3961 gcatgaccgc gcggcttccg gcgcgccggt gcgctgcact ttgagcgtgg aaaagcagcg
     4021 cggcgtggag agggcgctcc aggattatgt ggagcgcaag cgcgaggtgg gcatcaccaa
     4081 tgcctgcgcg ctgctggtcc acgcgccctc gcgcgaggtg ctcgcctacg cgggttccag
     4141 ccgctttctc gacccctcca tccagggcca ggtggatgga gtgacggcgc ggcgctcgcc
     4201 gggctccgcg ttgaagccgt tcatttacgc gctggctttg caggagggct tgatccaccc
     4261 gcgcacgctc ctgcgggacg ggcgcacggc cttcggagct tacaacccgg agaacttcga
     4321 ccgcaacttc gccggcccca tctccgccca ggacgcgctc ttccacagcc ggaacattcc
     4381 cgccgtggcg ctggcgcaaa agctggctcc tccgggattg tacggctttc tcaaagcggc
     4441 gggtgtggcc ctgccgcggc cggctggaca ctacggcctc gctttgccgc tgggaggggg
     4501 cgaggtctcg atggaggagc tggcggagct gtattcgctg ctggcggatg acggccgcgc
     4561 ccgccgcctg agcctgcagc ccggcggaga gctggagcat caaagccgcc cgctcatcac
     4621 cgcggaagcg cgccacctga cgcgcatgat gctcagcgcg cgcgaggcgg acccggccat
     4681 ggacgacccc tccgtgatgt ggaagacggg cacctcccac ggcttcaggg atgcctgggc
     4741 ggcgggcatt cgcggcaatt atgtgctggt ggtgtggatc gggaatttca acggcaaggc
     4801 caacccggca ttcatcgcgc gggaatgcgc cgcgcccctg ctcttcgagg tcttccgcca
     4861 tctgcgcctg cccgcgcgcg ccgatccgcc cccgccgggt gtcgcccgtg tggagctctg
     4921 cgccgtgtcc ggtcaactgc ccgtgccgca ctgccgccac cgcgcgcgcg gtggcttcat
     4981 ccccggagtc tcgcccatca cgccttgcgc catccaccgc gaggtcctgg tggacgcggc
     5041 atcgggcctg cgcgtggcgc gtgatgacgg cagccgcgcg ctgcggcggg aggtctttga
     5101 attctggccg ccagatcttt tggaaatgtt caggcaggcc ggcgtgccgc gccggacacc
     5161 gccgccgctt gagttcgact cggactccct ggccgccgcc gacatcgccg cgccgccgaa
     5221 aattttgtcc ccgcgcgcgg cgctggttta cactctgcgt gcatcggaac cggcccggca
     5281 gacgatcccg ctgcgcgcgg acaccgcgcc cggggtggcc cgcgtctttt ggttcgcagg
     5341 gccgcgcttt ctgggcgcca gcgatcccgc cacacccctg ctctggagcg cctccccggg
     5401 cagatggcag gtgcatgtgc tcgacgatca cgggcgcggc gcggcggtgg aggcgcgggt
     5461 ggagatggtg ccgtgagggt cgtggggaac gggcgtcccg cttgttcaaa aacacgggcg
     5521 gtttatctcc ctttccttgc cttgcagaca tcccgcgagc gcaaaagccc tcgccccttg
     5581 ccaaagcggc catccgcttc attgtcccct ccctttccgc ccgggcggcg gcatttcgtc
     5641 ttttcaccct ctgtcatgtt taactggatc atcaaaaaaa tcatcggcac caaaaaccag
     5701 cgcaccgtga ggcgcctgca gccggtcgtg gccgagatca accgcatcga agccgaactc
     5761 cagaacctgc ctgatgaggc cctgcgggaa aaatgcgccg cctggaaaga gcagttccgc
     5821 gctttccaca ccccgcgatt tctcggcggc gtctccctgc gcatcgcctc ggaggaggag
     5881 gtggacgcct gcctcaggca tgtggccggc tacttcgccg cgctgcaaaa acacttcccc
     5941 tccctcgccg gcgactacct cgccgaatcc tcctggaacg gttcggacct ggatgacaaa
     6001 aaggcccgca ttgaccgcgg ccgcgaggac tggaatgaaa tccagccgcg ctttgccgcc
     6061 attgaggcga agatgctcga tgacctcctg cccgaggtct atgccgtggt gaaaaacgcc
     6121 gcccgccgcc tttgcggcca ggagcacagc gtgtgcgaca cgccgctgaa gtggaacatg
     6181 gtccacttcg acgtgcagct catcggcggc gccgccctgc atcgcggcat gatcgcggaa
     6241 atggccaccg gcgagggcaa gacgctcgtc ggcaccctgc ccgtcaccct caacgccctc
     6301 accggtcgcg gcgtccacgt catcaccgtc aacgactacc tggcacgccg cgactcggag
     6361 tggatgggct atttgtacaa gttcctcggc ctcagcgtcg gctgcatcca gaacgaccag
     6421 ccctcgcatg tccgccgcga gcagtatgcc tgcgacatca cttatggaac caacagcgag
     6481 tttggcttcg actacctgcg cgacaacggc atggcctcca gccgcgagca gcaggtgcag
     6541 cgcgggcact acttcgccat cgtggacgag gtggactccg tgctcattga cgaggcgcgc
     6601 acgcccctca tcatcagcgg ccctgtcgcc ggagcggaca gcacgcatca gtacgagcgc
     6661 tacaagcccc tcgtggagca gctcgtgcgc cgccagcaga ccctctgcaa cagcctcgtc
     6721 accgaggcca aggaactcgc cgccgccgga gacctcgagg aggccgggcg caagatgtac
     6781 caagtccacg tcggccagcc gaaaaaccgc gccctcatgc gctgcatgga ggaccccgag
     6841 ctgcgccgcg ccatggagaa ggccgagctg aaaatgtacg aggacacgca gaagaaggag
     6901 ctctttgccc tcaaggaaga tctgtttttc tccatggacc cgaagtccca cgacgccgac
     6961 ctcagcgaga agggccgcaa tttcctcaat ccaaacgagc cggacgcctt cgtgctgcct
     7021 gacctcgcca cactttactc cgagatagac ggcgacacct cgctcaccga cgcgcagaag
     7081 ctagccaaga aaaacgagct ccaggaccgc ctctcccacc agggccagcg catccaccag
     7141 atcagccagc tcctgcgcgc ctactgcatc tatgagaaag acgtcgagta cgtcgtcgag
     7201 gaaaacaaag tcatcatcgt ggacagccag accggccgca aaatgcccgg ccgccgctgg
     7261 agtgacggcc tgcaccaggc cgtggaggcc aaggagggcg tgcagattga cgccgagacc
     7321 cagaccctgg ccaccatcac catccaaaac tacttccgcc tctatcaaaa gctcggcggc
     7381 atgaccggca ccgccgagac cgacgccgcc gagttccacg acatctacgg cctcgacgtg
     7441 ctcaccatcc ccacgaaccg cccggtgaag cgcctggacc agaacgacag catctacaaa
     7501 acccgccgcg agaaatacgc cgccgtcatc gccatgatcc acgagcgcca tgccaagggc
     7561 cagcccatgc tcgtcggcac cgccagcgtc gaggcctccg agacggtcag ccgcatgttg
     7621 aagctgcaaa aaatccccca ctccgtcctc aacgccaaat accaccgcca ggaggccgag
     7681 atcgtcgccc gcgccggcca gaagggcgcc gtcaccatct ccaccaacat ggcaggccgc
     7741 ggcactgaca tcaaactcgg cgagggcgtg ccagacctcg gcggcctctt cgtgctcgcc
     7801 accgagcgcc acgagtcccg ccgcgtggac cgccagttgc gcggacgctg cgcgcgccag
     7861 ggcgatcccg gagagagcaa attcttcctc agcttcgagg acgacctcat gcgcaacttc
     7921 ggtgccgccg accgcatgac gaagatcatg gagcgctttg gcatgaagga gggcgaggaa
     7981 ctccagcacc cctggctcaa ccgcagcgtc gagaccgcgc agaagcgcgt cgagcagcgc
     8041 aactacacct ggcgcaagcg cgtgctcgaa tacgacgacg tgatgaacca gcagcgcgag
     8101 gtcatctacg agcgccgcaa cgacgtgctc aacagcaacg acccccgcct tttgatcaac
     8161 gacgccgtca aagaaggcat cgaggagcgc ctcgaggagt tcctgcccaa ggacgccggc
     8221 aacgacatgg agcccgacca cgccggcctc ctgcactggg tgaacaccac cttccccatc
     8281 ggcctgcgcg agctggacga cgccttcaaa gcgctcgact accaggccca gtgcgactgg
     8341 atccgcgaaa aagtcctcca cgcctacgac gccaaagtcg ccggcgccaa cccccaggcg
     8401 ctccaggaga tcgagaaaat gatcctgctc aacgccattg accgcctctg gcaggagcat
     8461 ctctacgcgc tcgacgccct caaggagggc atctccctgc gcagctacgg ccagaaagac
     8521 ccgctcatcg agttcaagca ggaggccttc tccatcttct ccgaactcat gcgcagcatc
     8581 aacggcgagg tcctcggcaa cctcttcaaa agcacccagc agctcgccgc cttcgagcag
     8641 ttcctggccc agctcgccat gcagcagcag caaggcggcg ccgcagccgg ccagccgccc
     8701 cagcagcgca agcccgccgg agaaccccgc ccgcatgatc ccgcgaagga aggccccaaa
     8761 ctcatccttc ccagcgccgc gcccgccccc cagaagccca agtacagcgc cggccgcaac
     8821 gacccctgcc cctgcggcag cggcaaaaaa ttcaagcaat gctgcgggcg cgtcgcctga
     8881 gcctggatgc ggccccatcc gcggcaggca caggcatgac caccgtccaa atgagccgcc
     8941 atccgccgcc gggtttcccc agagcggaaa aagcgcgcat ccccaacccg gcccaccagg
     9001 cacgccgcca ctgagggcgt gactggctgg aaatgaagcg cccccgactc cggcgcagca
     9061 ccgccgaagc gggaaaacat tgcactgttt tgccagaaca gtgaaccgtc gcggaaaaac
     9121 tttgcatcgt tttgccagga ctttgcaagg ttttgccagg aaagggtgcc atcgcgggaa
     9181 aacggtgcgc tttttttctg ctcaagcgtg cctcgtttgc gtgcaggcgc gctttttttc
     9241 ctgggtggac gccggtttgc ggctgcggtt gtagaaggtc tcgatgtagt cgatcggtgt
     9301 gaaattgagc gataggttga tcttcagcga gttgtgattt tggcatctca tcattcgatt
     9361 attcacaatc ccttgaaaga atagcttgct gacaaaactc ggctcacctg ctataaagcc
     9421 gctgatattt ggataattca aaaaagtatt tcattcggaa aggaaacact taacgcgaat
     9481 ctctcatgaa cactcgaatc agtccgtctg gaaccttttt gcgggacttc tcaagctggt
     9541 tgaaccgtta ccgaatttta accgcaggca ttttgatcat gtcagcgcct gccgcgctct
     9601 cggagatcac attgggcagc acgtcgtgca gctacctgcc tgccaagtcc ttgaagacga
     9661 caacccagtt gagcagtgtc ctcgcctcgt ctccacgcat caggttcgtt ggcaaggtgg
     9721 gtggagtggc ttttgacgcg gtggctgttc ctgattccgg tctcgtgatc aaaagcctgt
     9781 ctcttgactt ggacactgcg agaccggatg gttcaaggct ggttctgacc gtaaacggtc
     9841 gtccggtcaa aacgcggatt catgattggg aactcctgcc caccatccgt tacgctgaca
     9901 gtgactcgac aagctgcttc actctctttg gcgaacttca agatgaggag gagggacgaa
     9961 ggctgcgggg tgacggcgcc cgtatcttaa attatgacga cgcattccaa gactctctca
    10021 tgggactgag aatgttccaa ctcgacatcc tcctgctcgg tgacgacttt gccaccgacc
    10081 tgccgaagga ggacggcgaa tacatcctgg gtgcgggtga aaagtccccc gacctttcaa
    10141 cggccaagtc tttgcatgaa aaatatgagg aagcacgcct cgaactggat ttgaaaggca
    10201 tccgggagga cttcgacacg gagtttcaat cttacgtcat caccgatcag tcggtggaca
    10261 tccggtttgt catcaaggac gggcagcttg agatcacggg cgagcctttt tattattttt
    10321 ggaaggatga cgaggacgat ttaaatggcc gttaccaggc ggcccaggcc gaagaggctg
    10381 tcaaggcggg cgtgctcaag ccaggggatg ggtttgatga actgcgtgcc atgatcatca
    10441 aggagcgcaa ggctgatcag gagcgaaaag actccgtcaa actcaaagtc gcgagagaat
    10501 tgaaagtaat ctcagacacc ggcactctgg aggacttgga acagatggag actgaggcgg
    10561 acgaaaagta tgaagcggcc ctgaacaagg ctgtttatga aatcgccaaa gcggaaggca
    10621 aagtgcgcga gggggagact cccgaatctt ttgcggaacg catcttcgaa gagtacatgg
    10681 aagcggacgc ctcagtggaa aacgccatcc tggagcggaa ccagaattgg aggaacaaag
    10741 cggctgagaa cattccggag aagaaagccg agcacgcggc tttcaaaaag cagatggacg
    10801 acatcaatgt ccaagtcgct gggatcaatg ccgggcctga tgcggcgtat gcggctttgg
    10861 aggacaaact ggatgagatc agggacaagg ccaggcagac agtctcggag ttccaggact
    10921 cggacgaggc gaccggcaag tatttgaagg aattcagcgc cgctcatgcg caattcgtgg
    10981 atactctggc tgcgatgaat cccgccgttt ggaacgccgg gcgcacggtc atgcgttacg
    11041 gtgccttctt ccgctactgc agggcgcatc attctgatgc ctggtccgct ttcatgcaga
    11101 agttgggggg gaatgaaggc aaactggaga ccaagcctgc ggtcaagaca ccgacggtga
    11161 tgaaattcag ccctccggcg ggtcaataat cacgccatga gtgctgagag tcgcacggag
    11221 cctctcgtgt ttgtcagttt cgcgtccggc gatgacgaag cgcgcgagga gttgggacgt
    11281 gaactgcaca ggcagggtat caaggcctac gactacagca tccctcacga aggcgaccaa
    11341 gtgggattgg agatcgtgtc ccagcttagc gagaaaatcg aacaagcgcc gtttttcatc
    11401 gcgctggtct catcggccag caccaatcct gtgaagcaca ttgtgcatgg agaggtgcgg
    11461 cacgcttttc gccgcacatc tctgccggaa aaaaacagat gcgtttgcgt ggtactgcgc
    11521 ggtcggcgag gggacggttc ttcctatgac ccgccgaatt attggaagct ggcgcttgcc
    11581 gcccttaaca actacaacgc cagtttcccc gattttgcca tcaaggttcg cgggctgaac
    11641 aaaaccgacc cgaactgtca tggcgtggtc tgttttcgct cgcggggaac agtggccgat
    11701 ttcgattttt ccggaacact tgaagacaaa gaggcatggc cggcggtttg ccatgaggcg
    11761 gaactttact ttgaggatgg gacatggaat tcgcttctgg ccgcgctgcc gcccacttca
    11821 tcttttgccc tgggttccct ggccagaaag aacgcttgga tgggcattta cagccacgtt
    11881 caaggccgcg cgtggcctca tgtggacaat gtcgcgccag gctgcttcaa ccgtgtggtg
    11941 atgacagtct gcaaccggct gggagtttcc tacacaagac ccgtttcgcc ggactcaagg
    12001 ctgcctttct acgaccaact caagaaggag gtggcggcca tgccgctgaa tcagagcatc
    12061 agcgtgctgg gacagctcga ctacttcgag tcgcggatca tggtcggctc ctggcacgac
    12121 gcgctggttc acatacgatc cctgctcgcg ctgttgcgtc aaaacaacgc gcgcagccgt
    12181 ttcgccgaga ttattgaagg agtctgcctt ctgcgcctgg ggcgctcgaa ggacgcgctg
    12241 agcatctacg aggggctgat ggacagaccg gatgaaaaca tctgcatcat gcgagcgaag
    12301 gcgctgaccg ccgagggccg ccatgccgag gcgctcgccg ccgccaggcg tgccgtcgag
    12361 atctgcgatg ccaagcacga tcccgccgcc ctggctcatc aggaatggct cacgctcgca
    12421 gtgggcagcc aaaaccgcgc gggcgcggaa agtttccttg ccgatgacgc cgcctggctt
    12481 cgggaggtga gtctggccaa tgccagggca ccccatattt tccgaagcgc gcctgaaaat
    12541 cttaaaaccg aggaccgtct gaaactgcgt tgtctcgaag cacactggtt gaagctcgcg
    12601 ggaagacgct ccgaggcgtt gacgaaactc gaaaatctgc ttgcagaacc cgtcgtttca
    12661 gaagtcgtga ggctggctgc gggagatctt gaagggctgc cggaccaact gaccaaccgc
    12721 ttttatgcca ggctggctga tgccggcatt tacctggaca gcccgctgga ggaacggatt
    12781 ggagaggacg gcatgacacg catcaccgcc aacggcaacc gggaggaatt ctggctgcgc
    12841 gagcaggacc gggtatattt tttcaagcag caacagccct ggcactacac catccgttac
    12901 ctcgacaagg ggaccgccat gctgcgctat gaattgcgtc tacttccccg ccgcgaggat
    12961 gacatggcgc tctggcacgc cgcgttgctg gaattggaag acacgctgga atgcctgcag
    13021 gacctgcacc tttgccgcca tgtcatcaga gagtgggcgg agtttggcgg cctccacgcc
    13081 gcgtggcgtg ttttccttcg ctcccggctc tggttccccg acgatcccta tctgctcaat
    13141 gatggcgccg cgatcgcggc ggcagcggag gatggggagg cattggagca tctggcgtcc
    13201 gaggcggagc gcgcggcccg tgctcggttc gcgagatgcc agcctgccgg gactgagtgg
    13261 actccggcgg agcttttgca ggcttgggcg gaatctcaga atctcctggg ccgggacgct
    13321 ttgtccgctg cggttcaccg cttcctgaat gacgcatgat ccagccttga ttcttgcctg
    13381 cttgttccaa aaccaaccag aagcccaatc ttatgattcg tacagaagtt cattcccgct
    13441 ctcgcacttc ttcgccacca gctcgccctc cttcaccggc agcttcggca ggcgaaccgt
    13501 ctccggcagg tgttcctcga acacgaggcc gaacttcttg ttcttcttca gttcccgcac
    13561 ttcggccccg atggcctact tcagccgttc gtccgggatt tgagcgatga aatcttcgat
    13621 cttcgccatg tcagcgcgcc catcccggct gaagattttt tccggtggag gaattttttt
    13681 ctcgacgctg gaggcgggat tcctttattt ttttcggtaa attccagcca tgaacaggag
    13741 tttgccttcg ggctgccaga cggctttcag ccgccagccc cggcttatgg ctcaacggcc
    13801 agcggccaag agaggcgcta agcgcggtca ggggatttta tcggggaggc atggcgcgcc
    13861 attgtgggcg gttgccgggc tggcgcaagc ggggatgaac tcacgggggg agggtagggg
    13921 cggtttcccc ggcgctggag aattgttctc gggcactgga gagtcgtttc cgggctctgg
    13981 agaatcgttt tcagtcgctg gagaatcgtt ctcgggcgct gggaaatcgt tctcggactc
    14041 tggagaatcg ttctcgggct ctggagaatc gttttcgggt tctggagaat tgttttcagg
    14101 ctctggagaa tcgttttcgg gttctggaga atcgttttcg ggttctggag aatcgttttc
    14161 ggacgctgga gaatcgtttt cgggcggcct gaaaggcatt tggaaacacg ggaggagcct
    14221 cgttaaatcc atgaaaatca acattttgcc atgccgacct ggtccagcgg agtgacttgg
    14281 tcctccggcg ccctttgggg gccggccacg gggccgaatc cctttcaaaa ccgccaaaag
    14341 ctgccaggca tctgcgtcac gggttcggga acttctcctg gacgggcggc ggggtttttc
    14401 ggctcatccg ggaagtgggt gcggagtttg ggttccgcgc ggcagccttt ggactgcggc
    14461 agcctgctgc cgctttcgga ggaagacgtg agtcttcccg gatgggaggc gcgggcggaa
    14521 tggggcgtct gtgccgggag cggggcaaac tgacgtttgc ctctacgggc ggcgcggggg
    14581 cgcgtgtaga ggaagacgtg agtcttcccc ggatgggagg cacgggcgga atggggcgtc
    14641 tgtgccgggg gcggggcaga ctgacgtttg cctctacggg ggggcgcggg ggcgcgggta
    14701 gaggaagacg tgagtcttcc cggatgggag gcgcgggcgg aatggggcgt ctgtgccggg
    14761 agcggggcaa actgacgttt gcctctacgg gcggcgcggg ggcgcgggta gagaaagacg
    14821 tgagtcttcc ccggatggga ggcgcgggcg gaatggcgcg tctgtgccgg gagcggggca
    14881 aactgacgtt tgcctctacg ggcggcgcgg gggcgcg
//
LOCUS       DAOMYJ010000123        14842 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Prosthecobacter sp. isolate AMR_MDS_5629
            NODE_1378_length_14842_cov_24.629404, whole genome shotgun
            sequence.
ACCESSION   DAOMYJ010000123 DAOMYJ010000000
VERSION     DAOMYJ010000123.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867850
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prosthecobacter sp. (wastewater metagenome)
  ORGANISM  Prosthecobacter sp.
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Verrucomicrobiaceae; Prosthecobacter.
REFERENCE   1  (bases 1 to 14842)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14842)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.30
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:34:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,742
            CDSs (total)                      :: 4,684
            Genes (coding)                    :: 4,676
            CDSs (with protein)               :: 4,676
            Genes (RNA)                       :: 58
            tRNAs                             :: 55
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14842
                     /organism="Prosthecobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1378_length_14842_cov_24.629404"
                     /isolate="AMR_MDS_5629"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1965333"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..391
                     /locus_tag="PLP58_13290"
     CDS             <1..391
                     /locus_tag="PLP58_13290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ09545.1"
                     /translation="AVLTTWAVRERNLLRERVAELTPRASQVLEQKRAWQEAAPAADP
                     GISPMQVLLDVMQPEVSTEISMTNFEWTPERVLMRGRTPTPALALEYATGIKDAEPLA
                     RFTWETPAPVIASDNSATFELKGEVRP"
     gene            388..960
                     /locus_tag="PLP58_13295"
     CDS             388..960
                     /locus_tag="PLP58_13295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ09546.1"
                     /translation="MKKSERILLVIFTLVFLVVVGGGLLAFGLKNYRELTAETGRLRD
                     RLAEMHQALAESAVWQERSAWLDEKMPRFASRQEASAHLLENLQSTAENAALALGPRE
                     FIEPQRLDGEEDAPQGFYDKATVRLTLDQAPENALFTWMHALQSSGEFQGVTRLSITP
                     SGQGKTVNAEIEVTQFYLEKPHAKVTRRDR"
     gene            978..1505
                     /locus_tag="PLP58_13300"
     CDS             978..1505
                     /locus_tag="PLP58_13300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ09547.1"
                     /translation="MRAALLFPLLITAARAGDALVKPPPLEHYAALWERSLFTTRDLP
                     TPDSPAGPLFTDNLSLAGVYEVNGAVVAVLVDRASSNVMEARIGSENSAGIKIRQIEP
                     GETMDKTRVQLQKGDQAGWVGFDNSQPTSPSSPQPALESRPAVPARELPPSPLLPSIS
                     GRPPSSSGDVPLPPP"
     gene            1555..1947
                     /locus_tag="PLP58_13305"
     CDS             1555..1947
                     /locus_tag="PLP58_13305"
                     /inference="COORDINATES: protein motif:HMM:NF023178.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3307 domain-containing protein"
                     /protein_id="HRJ09548.1"
                     /translation="MLDLVEFPPATLLAAAQVFFALCIGHAVADFPLQGEYLAIGKNR
                     RFLLRLADPARPASIWVACMGAHCLIHAGMVWIITGSALLALIEFALHCLIDIAKCEG
                     VTGFNTDQLLHLACKALYVAAAWFGWLA"
     gene            2003..3196
                     /locus_tag="PLP58_13310"
     CDS             2003..3196
                     /locus_tag="PLP58_13310"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018970656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HRJ09549.1"
                     /translation="MPVPLLDVNAQNHPLESELLEAFTRVLRHGRFIMGPEMETFEKE
                     VADMLGCRHALAVSSGTDALLLGLMALDLQPGDEVLCPAFTFFATAGAVSRLGAVPVF
                     TDVCPVCFNMDVSDARAKITPRTRAVIPVHLFGQSADMDALLALAKDNNLRVLEDGAQ
                     AIGATYKGRACGTMGDFGAFSFFPSKNLGGFGDGGMLVTNDDSLAEYARVQRVHGMKP
                     KYYHHHVGGNFRLDTIQCALLNVKLKHYATYTAQRQANAAHYAEALSKLPGVVTADPA
                     HCKCPASQEAWLTANTARLILPAAYPHNTHIWNQFTLRVPGEGRRDALRNHLQAAGIG
                     CEIYYPLTLDQQPCFAHLPESSRTGCGVAHRMAGEVLSLPIYGELTEVQRGEVIAAIA
                     SWLKH"
     gene            complement(3332..4108)
                     /locus_tag="PLP58_13315"
     CDS             complement(3332..4108)
                     /locus_tag="PLP58_13315"
                     /inference="COORDINATES: protein motif:HMM:NF014035.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HRJ09550.1"
                     /translation="MTLNDWLTQHTLFTGDAHVMLMAGAAALCIGLSKSGLSGTATLN
                     VVLMAQAFGARESVGLVLPLLIVADFMGYFINRHGGGWRQVLPMAPPAIAGVILGWLL
                     LGHIDNQAARVVIGWVIIALLCFKLLLDARRQDFIALTEHRGFAWTMGLGAGVTTMLA
                     NAAGPVMTVYLLSRRMEKKDHLGVFSRFFLFINLFKVPFSANLGIIHPRSLATNLVML
                     PAVVLGILLGWQILKRIPQKPFEHTLFVLTLVAAVWMLLG"
     gene            complement(4118..4717)
                     /locus_tag="PLP58_13320"
     CDS             complement(4118..4717)
                     /locus_tag="PLP58_13320"
                     /inference="COORDINATES: protein motif:HMM:NF015679.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HRJ09551.1"
                     /translation="MKAVHLLLIAASALSLTACQIMPLGSTKEVVRRAIYVLPADSSS
                     SPLYVWHGTGTPGPVRVTIDLSQQKAYIFRNNQNIAWSYVATGRSGFRTPTGTFRISE
                     KVVDKRSNKYGSIVNANGVVINGSATAGIHRVPSGGRFVGAKMPYWMRLTGNGVGMHA
                     GPIPHPGSPASHGCIRMPYPMAQKLYAIAPYGTPVTIVP"
     gene            complement(4785..5969)
                     /locus_tag="PLP58_13325"
     CDS             complement(4785..5969)
                     /locus_tag="PLP58_13325"
                     /inference="COORDINATES: protein motif:HMM:NF024907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FG-GAP and VCBS repeat-containing protein"
                     /protein_id="HRJ09552.1"
                     /translation="MRTLALSVIFLPSLALSQVQAQFRELEIDAEVGIGYGIALADVD
                     GDGRTDILLADKDAIVWYQNPAWQKHDIARKLTDRDHVCIAARDIDGDGKAEVAVGAQ
                     WNPGDTENSGAVFYMKPPADRTQLWTPVQLTHEPTTHRMRWVRNRAGRHDLVVAPLHG
                     RGNKNGEGAGVRLLAYHVPENVDGTWNTTLIHDSMHMTHNFEVVPAPAHEAETLLLAG
                     REGIVRLTPGDSGWRSQWVSNHTEAGLAGAGEIRWGAFAGGKPWIVTIEPMHGHELVL
                     YTPAADGPKDGLWQRRVLDDTLVDGHALACADYLGLNNRQIAVGWRSAQKVGPKVGVK
                     LFYTSKEDGNGWQQHLVDDNGMACEDLMPADLDGDGDTDLVAAGRRTKNLKIYWNLRK
                     AP"
     gene            6193..12510
                     /locus_tag="PLP58_13330"
     CDS             6193..12510
                     /locus_tag="PLP58_13330"
                     /inference="COORDINATES: protein motif:HMM:NF012387.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="immunoglobulin domain-containing protein"
                     /protein_id="HRJ09553.1"
                     /translation="MHFPFVHAFHPRLIAMSAAVLLLTPAAWAIPPAAPTNVKASVSL
                     KVANGTTALAHDKYLVTWTDNAVDETGYRLEIRFGNVGQFLTLALLEPNTEGVLWTPT
                     ASQFSAVAGQIIQFRVIAFKYNAAAVELRSGPIQTLTYPSTTGNISKPTGLTAVNVGD
                     GGVRLEWNDTSNAETYHQVFYKKSSEGTYQKSFYIEHNVSDVALLSGRLPPSKLPHLL
                     TGARANAAVHFEPGATYNFRVRSVVRLNQDTGNFHNASEARNAPYDSDLSDPVSITIP
                     ALSPPTELSGSVVDQSSIRLNWVDNSSNETGYKIEYKAIGTSTWTTLGSANGAAPLNS
                     TSYTVNIGSGVTLDWRVSAVYSDPVSGSVVATSATSNEYPIGTTFPGPGNLTATDTGY
                     SGKILLEWQDNAEAETNYEISARVKGTTTWRYVTTLAADARRAHVSQAEFDGASLSDF
                     PKGEALEFLVEAVVYVRDSNGNAISRVTAQDGATVEATARHGFTSRPYHPISEGVFFT
                     YTATTSNADERVSWNVTGLPDGVTFDSMTGIISGTPAEAGHFPSVMTATFSDGHVANA
                     ILNLRVLPASSEPQTAAAIQNVTLAPGVQFFLPLADKFSDTDSKAAARLRTTLGEIDI
                     LLYPDLAPQSVQNFLHYINNGGYNGVVFHRSVPDFIIQGGSYRAFQAPDYFVKLDASR
                     PSPLNEPGISNLRGTVAWAKLPGTADSATLDFFFNLGNNAENLDNQNEGFAAFGRVVG
                     AGMDLVDDIAGFPIGTYRNFNAAGGTNAALDKRVNLVDFSESGQPFLSGKEALDAVPM
                     NTEAENAPLDMDVNQTVRILQAAQISPLGYSVPANTAPDVVSAFVSGGNLVIQGLKEG
                     VSSITVQAADLDGHLVTQTFTVTIVRNYKAPVITKQPVSVAVLPGADAKLAVTATGTG
                     PLNYQWMKDGQDINGENSAKLTVAAVDAGKTGFYAVRVWNNSLTVLSNVVRVDLRAPP
                     VFTTHPVAKVVEAGKPLELEVNGTGAPVPAISWLRSGKVVAKQTSTKLSIPSAKIADG
                     GVYTARASNVVAKNVSSQPAEVLVVDKGAKLAFAPAGRKLALQAQAAAGPGLLHQWLR
                     NGGLLLTDTDNVTGVQGPVLTIKALGFGDLGSYTCQVLSADGSLTAETGPWKVSIPQK
                     PVLQNFTAATAYVGLDYEFTPAFGGDANNSVASFSIKGLPGGLKYDTATARIFGRPTH
                     PGVFTLTVTGKNPAGTSAPVTGMLIVTPMPEPAVGTFVGQVAASSTLNGGKGGRMDFT
                     VTDGGLVSGKLTVGKDAFSFKGTMRQTPGSSLATGEAVIARKGGALPVTFTFSIFALQ
                     GFADSGNFEGTLSDGVTSAQTVGYRKVFHATWNPYSLPQSYNLGLNLANVEDVDKDAI
                     PQGSGFLRVVTNTSGDASITGRLADGTTLTCSSPLGGVLAGGQNIRFLVYQSLYKHTG
                     AIVGEPGILVLQLAPAGVNDIWFRVSGQLRWTRDAQTAAAERAYKPGFGPVILDALGM
                     TYTPPASGSPVFRLPKSGEDNARMEFEHGGIEHAATDPDVPLLTITNANGVSIAAANN
                     PGGVKLTITPGTGLLSGSFTVEDDPAVTPKRGATFHGIIVPQIPDFQIQQTDQIGNTT
                     PVEVPGSPARGIGHFLLAQKPSAGPPATTAKTSPILSGIFRLLPAPITINTQPQGATK
                     DPGENHTFTVAVTAPGGTVTYQWRKNGLSITGATNATLVLTGISEGNEGTYDVVISTE
                     YSRVVSDPAVLNVNNIISEVIITRSPSDEVLDEGGSVTFTAVANGSGTLSYQWRKDGD
                     DIEGATSSTYIIDELMAVHSGQYSVRVTSALTPGGFVSNVLDLQVATGVVNPLLARLP
                     ATTDVSIGSSVTFTVTPDGRPPHFFTWFKDGELIEGASNAPNYIIHFVTQESAGNYSV
                     LVSNALTPEGVYTNTVPLGVDDKVSNVVATRAPDEEAVAESTAVEFTVTHSGAGPFTY
                     QWYKNDVPIDNATGISHSLPAALEADSGNYRVRVFNASTPEGVLSDPVPLVVAQPVSS
                     VSAALTLPLEGPIVEGGTFRITATANGSEPRTYQWYRGGVPIDGATGSQLTISGATPE
                     DHDGEYAVSVSNPVTNEEFSNNVAIQVQPEP"
     gene            12940..13137
                     /locus_tag="PLP58_13335"
     CDS             12940..13137
                     /locus_tag="PLP58_13335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ09554.1"
                     /translation="MKTALSPDPPQDFLDDPYAHPLADSYRFFANLTLESFFAHGNNL
                     ATVMQVLKREPDEYYPAPERP"
     gene            complement(13188..>14842)
                     /locus_tag="PLP58_13340"
     CDS             complement(13188..>14842)
                     /locus_tag="PLP58_13340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009961489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transglutaminase family protein"
                     /protein_id="HRJ09555.1"
                     /translation="ASWRQGIHAGQGKPERRKLPPLEPEENDPSSPTGTRFPRLQESA
                     SWIIRTALCFEPREGRLHVFMPPVAATEDYLDLISAIEDVAATLAIPIVIEGTSPPYD
                     PRLHVIKVTPDPGVIEVNMHPVKTWGDLVENTTALYEEARLTRLGTEKFMVDGRHTGT
                     GGGNHIVVGGESPRHSPFLRRPDLLKSLIGYWHNHPSLSYLFSGLFIGPTSQHPRVDE
                     ARNDALFELELAFKELDRQTATGGFTPPWLVDRIFRNLLVDATGNTHRSEFCIDKLFD
                     PGSSTGRLGLVELRSFEMPPHAEMSLAQHLLLRAALARFWQQPYEAPLVRWGTELHDR
                     FLLPHFVWQDFSDVIEDMADFGYPLSADWFAPHFEFKFPTVGSITGRGVHLDLRTALE
                     PWHVLGEDGAPGGTVRYVDSSLERIQVKTAGLVNNRHLITCNGRIVPLHPTGTVGEYV
                     AGVRYRAWQPPNCLQPTIGVHSPLVFDIVDTWNQRSIAGCTCHVAHPGGRSYDTFPVN
                     SYEAESRRLARFFPGGHTPGKMQPVAVPPVPEFPLTLDLRRG"
BASE COUNT         2755 a         5068 c         4489 g         2530 t
ORIGIN      
        1 ggccgtgctt accacctggg ccgtgcgcga gcggaacctg ctgcgcgagc gcgtggctga
       61 gctgactccg cgcgcctcgc aggtgctgga gcaaaagcgc gcctggcagg aggccgcgcc
      121 cgcggccgat ccgggcatct cgcccatgca ggtgctgctg gatgtgatgc agccggaggt
      181 ctccaccgag atttccatga caaacttcga gtggactccc gagcgcgtgc tgatgcgcgg
      241 ccgcactccg acacctgcgc tggcgctgga gtatgccacg ggcatcaagg atgccgagcc
      301 actggcgcgc ttcacctggg agacaccagc cccagtcatc gccagcgaca acagcgccac
      361 cttcgaactc aaaggggagg tgcggccatg aaaaagagcg agcgcattct cctggtcatc
      421 ttcaccctcg tcttcctcgt cgtggtcggc ggcgggctgc tggccttcgg cctgaaaaac
      481 taccgcgagt taaccgccga gaccgggcgc ctgcgggacc gccttgccga aatgcaccag
      541 gcccttgccg agagcgccgt gtggcaggag cgcagcgcat ggctggatga aaaaatgccg
      601 cgcttcgcct cccgccagga ggcctccgcg catctgctgg aaaacctgca aagcacggcg
      661 gaaaacgccg cgctggccct cggcccgcgc gagttcatcg agccccagcg tctcgacggc
      721 gaggaggacg cgccgcaggg cttttacgac aaggccacgg tccgtctcac gctcgatcaa
      781 gcgcctgaaa atgccctgtt cacctggatg cacgcgctgc aaagctccgg cgaatttcaa
      841 ggcgtcaccc gtctttccat cacgccctcc ggccagggca aaaccgtgaa cgccgagatt
      901 gaggtcaccc agttctacct ggaaaaaccc cacgccaaag tcacaaggag ggacagatga
      961 tgagcatgca tcttgccgtg cgcgccgcgc ttcttttccc tctcctcatc accgcagccc
     1021 gcgccgggga cgcgctggtg aagccgccgc cactggagca ctacgccgcc ttgtgggagc
     1081 gcagtctctt caccacgagg gaccttccca ctcccgactc tcccgcaggt ccgctcttca
     1141 ctgacaacct ctccctcgcc ggcgtctatg aggtcaacgg cgcggtggtc gccgtgctgg
     1201 tggaccgcgc cagctcgaac gtcatggagg cgcgcatcgg ttccgagaac agcgcgggca
     1261 tcaagatccg ccagatcgag cccggcgaga cgatggacaa gacgcgggtg cagctccaaa
     1321 agggcgatca ggccgggtgg gtcggctttg acaacagcca gccaacctcc ccttccagcc
     1381 cgcagcctgc attggagtcg cgcccagcag tccccgcgcg cgagttgccc ccttccccac
     1441 ttctgcccag catttccggc cgtccgccct catcctccgg tgacgtgccg ctgccgccgc
     1501 cctgaattga ttttcaagtt gcgatgacag cccgtggatg gaagtctgaa aaccatgctt
     1561 gatctcgtgg agttcccccc tgccacactg ctcgccgccg cccaggtctt ctttgctctc
     1621 tgcatcggcc acgcggtggc ggattttcct ttgcagggtg aatacctcgc catcggcaaa
     1681 aaccgccgct ttctcctccg cctggctgat cccgcgcgac cggcgagcat ctgggtggcc
     1741 tgcatgggcg cgcactgcct catccacgcg ggcatggtgt ggatcatcac cggttcggcg
     1801 ctgctggcct tgatcgagtt cgccctgcac tgcctcatag acatcgccaa atgcgagggt
     1861 gtcaccggct tcaacactga ccagttgctg cacctcgcct gcaaggcgct ttatgtcgcc
     1921 gccgcgtggt tcggctggct ggcgtgaagg tttgccttct tgcccgttga ccggcggcgc
     1981 ttccacttga acttgcccgc ccatgcccgt cccgctgctc gacgtcaacg cccaaaacca
     2041 cccgctcgaa tccgaactgc tcgaagcctt cacccgcgtg ctgaggcatg ggcgcttcat
     2101 catgggaccg gagatggaga ccttcgaaaa agaggtcgcg gacatgctcg gctgccgcca
     2161 tgccctcgcc gtctcctccg gcacggacgc gctcctgctc ggtctcatgg cgctcgatct
     2221 ccagccgggt gacgaagtgc tctgcccggc tttcacattc ttcgccacag cgggcgcggt
     2281 ttcccggctc ggcgcggtgc cggtgttcac ggatgtctgc cctgtctgtt tcaacatgga
     2341 tgtgagcgac gcccgcgcca agatcacccc tcgcaccagg gccgtcattc ccgtgcatct
     2401 cttcggccag agcgccgaca tggatgcgct gctggcgctc gcaaaagaca acaacctgcg
     2461 cgtgctggag gacggcgccc aggccatcgg cgccacttac aagggccgcg cctgcggcac
     2521 catgggcgac ttcggcgcct tcagcttctt ccccagcaaa aacctcggcg gctttggcga
     2581 tggcggcatg ctcgtgacga atgacgactc cctggccgaa tacgcccgcg tgcagcgcgt
     2641 gcatggcatg aagccgaagt actatcatca ccatgtcggc ggcaatttcc gtctcgacac
     2701 gatccagtgc gccctgctca acgtgaagtt gaagcactac gccacctaca cggcgcagcg
     2761 ccaggccaat gccgcgcact acgccgaggc gctgtcaaag ctgccgggcg tcgtcacggc
     2821 cgatcccgcg cattgcaaat gccccgcctc ccaggaagcc tggctgacag ccaataccgc
     2881 gcgcctcatc ctgcccgcgg cctacccgca caacacgcac atctggaatc aattcaccct
     2941 gcgcgtgccg ggtgagggcc ggcgcgatgc cctgcggaac cacctccagg ccgccggcat
     3001 cggctgcgaa atctactacc cgctcacgct tgatcagcag ccctgctttg cccatctgcc
     3061 ggaaagctcg cgcaccggct gcggtgtggc gcaccgcatg gcgggggaag tgctgagcct
     3121 gcccatctac ggcgagctga ctgaagttca gcgcggcgaa gtcatcgccg ccatcgcctc
     3181 gtggttgaag cattaaaatc aaaatgcttg cgggatgagg ctgtctcaaa gcctgtccag
     3241 catttgcgac aaaacctgga gaagcagtga aattcggcgg atggaaaaat tccatatctc
     3301 atccaacgca agcctgcgcg cggattccat gctagccgag aagcatccag acggcggcaa
     3361 cgagcgtgag cacgaagaga gtgtgctcga agggtttctg cgggatgcgc ttcaaaattt
     3421 gccatccgag caggatgcct agcacgacgg cgggcagcat gacgagattg gtggccaggg
     3481 agcgcggatg gatgatgccg aggttggctg agaagggaac tttgaaaagg ttgataaaaa
     3541 gaaagaagcg gctgaagacg ccgagatggt cttttttctc cattcgccgg gagagcagat
     3601 agacggtcat gacaggaccg gcggcgttgg cgagcatggt ggtgacaccg gcgccgaggc
     3661 ccatggtcca ggcgaagccg cggtgctcgg tgagggcgat gaagtcctgg cggcgcgcgt
     3721 cgagcagaag cttgaaacag agcagggcga tgatgaccca gccgatgacg acccgggcgg
     3781 cctggttgtc aatatgaccg agaagcagcc agccgaggat gacgcccgcg atggcgggcg
     3841 gagccatggg cagcacctgc cgccagccgc cgccgtggcg gttgatgaag tagcccatga
     3901 agtcggcgac gataagcagg ggcagcacga gcccgacgga ttcgcgcgcg ccgaaggcct
     3961 gggccatgag cacgacattg agggtggcag tgcctgaaag gccgctcttg gaaaggccta
     4021 tgcagagcgc ggcggctccg gccatcagca tgacatgtgc gtccccggtg aaaagggtgt
     4081 gctgggtcag ccagtcgtta agggtcaagc cgcaggatca ggggacaatg gtcacgggcg
     4141 tgccgtaggg agcgatggcg tagagcttct gcgccatggg gtagggcatg cggatgcagc
     4201 cgtgggaggc aggcgagccg gggtgcggga tgggacccgc atgcatgccg acgccattgc
     4261 cagtgaggcg catccagtag ggcattttgg cgccgacaaa cctgccgccg gatggcacgc
     4321 gatggattcc ggcggtggcg ctgccattaa tgacgacgcc attggcgttg acgatgctgc
     4381 cgtatttgtt cgatcgcttg tccacgacct tctccgagat gcggaaggtg ccggtcggtg
     4441 tgcggaatcc gctgcgcccg gtggccacat aactccaggc gatgttttga ttgttgcgga
     4501 agatgtaggc cttctgctgg ctgagatcta tcgtcaccct caccggtccc ggcgtgccgg
     4561 tgccgtgcca gacataaagc ggggaggaag atgaatccgc cggcagcaca taaatggctc
     4621 tgcgcacgac ttctttggtg gaaccaagcg gcatgatctg gcaggcggtc aggctcaggg
     4681 cggacgctgc gataaggagg agatggacag ccttcatgac cgcggattct tgtgaaaaat
     4741 cagagaaaac gcaactcgct ggaatggtgg cgggccaagt taaatcatgg tgctttcctc
     4801 aaattccagt aaatcttgag attcttggtc ctccgacctg ctgcaacgag gtctgtgtcg
     4861 ccatcgccat ccaaatcggc gggcatgagg tcctcgcagg ccatgccatt atcatccacg
     4921 aggtgctgct gccagccgtt gccgtcctcc ttgctggtgt aaaacagttt caccccaacc
     4981 ttggggccga ccttttgcgc ggaacgccag ccgacggcga tctggcggtt gttcagaccg
     5041 agataatcgg cgcaggccaa tgcgtggcca tccacgaggg tgtcatccag cacgcgccgc
     5101 tgccacaggc cgtccttggg gccgtccgct gctggcgtgt aaagcacaag ctcgtgcccg
     5161 tgcatgggct cgatggtgac aatccagggc ttgcctccgg caaaggcacc ccagcgaatt
     5221 tccccggcgc cggcgagacc ggcttcggtg tgattcgaca cccactgcga gcgccagccg
     5281 ctgtcgccgg gggtgaggcg gacaatgccc tccctgcccg ccaggagcag cgtctccgcc
     5341 tcatgagcgg gggcgggcac gacctcgaaa ttgtgcgtca tgtgcattga gtcatggatg
     5401 agagtggtgt tccaggtgcc gtccacattt tccggcacat gataggcgag caaacggacg
     5461 cccgcaccct cgccgttctt gttgccgcgc ccgtgcaggg gggctacgac gaggtcgtgg
     5521 cgcccggcgc gattgcgtac ccagcgcatt cggtgggtgg tgggctcgtg cgtgagttgc
     5581 acgggagtcc agagctgagt tcggtccgcc gggggcttca tgtaaaagac cgctccgctg
     5641 ttttcggtgt cgcctgggtt ccactgcgcg ccgacggcca cctcggcctt gccgtcgccg
     5701 tcaatgtcac gcgcggcgat gcagacatgg tcgcggtcgg tgagtttgcg ggcgatgtcg
     5761 tgcttctgcc aggcggggtt ttgataccag actatggcgt ccttgtcggc caggagaatg
     5821 tcggtcctgc cgtcgccatc cacatccgcc agggcgatgc catagccgat gccgacttcg
     5881 gcgtctattt ccagttcgcg gaactgcgcc tgaacctggg acagagccag ggaggggaga
     5941 aaaatcacgg agagggccag ggtgcgcata ggccgtgatc atgcgcgcat ccggggggat
     6001 tttgccagca aaagctgcgg gaggttgaga aatctatgcc caaggtcggt ccgcagtctc
     6061 atcaaaaagt gacaatgtgc ataaaaagaa ttgacgatgt aatgacaaaa tcgctagcct
     6121 gatcgaacaa atcccccctt tttcggtgtc ccacactgga atcaaccggt ctgtgaacca
     6181 gccattcccg ccatgcattt ccccttcgtc cacgctttcc atccgcgcct gatcgccatg
     6241 agcgcggctg ttttgctgct gacgcccgct gcctgggcca taccgcccgc agcccctacc
     6301 aatgtcaaag ccagtgtcag tctgaaggtc gccaacggca ccacggcgct ggcgcatgac
     6361 aaatatcttg tcacctggac cgacaacgcc gttgacgaga caggctaccg tcttgagata
     6421 cgcttcggca atgtggggca gtttctgacc ctggctttgc tggaacccaa caccgagggg
     6481 gtgctgtgga cacccacggc aagtcagttc tcggcggtgg cgggccagat cattcagttc
     6541 cgggtcatcg ccttcaaata caacgcggcg gcggtcgagt tgcgctccgg accgatccag
     6601 actctcactt atccctcgac tacgggcaat atcagcaaac cgacaggact gacggcggtg
     6661 aatgtgggtg acggcggtgt caggctcgaa tggaatgaca ccagcaacgc tgaaacctac
     6721 catcaggtgt tttacaaaaa gagttcggag ggcacatatc agaagtcgtt ttacattgag
     6781 cacaacgtct ccgacgtggc gctgctctcg ggccggttgc ctcccagcaa gctgcctcac
     6841 ctgctgaccg gagcacgggc aaacgccgcc gtgcattttg aacccggggc aacctataac
     6901 ttccgcgtgc gctccgtcgt gcgcttgaac caggacacgg gcaacttcca caatgccagc
     6961 gaggcgcgca acgcccccta cgactccgat ctctccgacc cggtcagcat caccattccg
     7021 gcgctttctc cgcccacgga gctctccggc tccgtggtgg accagtccag catccggctg
     7081 aactgggtgg acaactcctc caacgagacc ggctacaaga ttgaatacaa ggccatcggc
     7141 acttccacat ggaccacgct cggatcggcc aatggagcgg cgccgctcaa cagcacctcc
     7201 tacaccgtca acatcggctc cggagtcact ctcgactggc gcgtcagcgc cgtttacagc
     7261 gatcccgtct caggctccgt ggttgcaact tcggcaacaa gcaacgaata ccccatcggc
     7321 accacctttc ccggcccggg taatctgacc gccaccgaca caggttactc cgggaagatc
     7381 ctcctcgagt ggcaggacaa cgccgaggcc gaaaccaact atgagatatc cgcccgcgtc
     7441 aaaggcacca cgacctggag gtatgtcacc accctggccg ccgacgcccg ccgcgcgcat
     7501 gtcagccaag ccgagtttga cggcgccagc ctctccgatt tccccaaagg cgaggctctg
     7561 gagttcctcg tcgaggcggt tgtctatgtg cgcgacagca atggcaacgc catcagccgc
     7621 gtgaccgccc aggatggggc cactgtggag gccaccgcgc gccacggctt cacgagccgt
     7681 ccgtaccatc ccatctccga aggcgtcttc ttcacttaca cagccaccac ctcaaatgcg
     7741 gacgagcgcg tctcctggaa tgtcaccggc ctgcccgatg gcgtcacctt cgacagcatg
     7801 accggcatca tcagcggcac accggcggag gcggggcact tccccagcgt gatgacggcc
     7861 accttttccg acggccatgt ggccaatgcc attctcaacc tccgcgtcct gccagcctcc
     7921 agcgagccgc agaccgccgc agccattcaa aacgtcaccc tcgcgcccgg ggtccagttc
     7981 ttcctgccgc tggcggacaa gttcagcgac accgattcca aggccgccgc ccggctgcgc
     8041 accacccttg gggaaattga catcctgctg tatcccgacc tcgccccgca gtcggtgcag
     8101 aacttcttgc actacatcaa caacggcggc tacaacggcg tcgtcttcca ccgctccgtg
     8161 cctgacttca tcatccaggg cggcagctac cgcgcttttc aggcgccgga ttatttcgtc
     8221 aagctggacg cctcccgccc ctccccgctc aacgagcccg gcatctccaa cctgcgcggc
     8281 accgtcgcct gggccaagct gccaggcacc gccgacagcg ccacgctcga cttcttcttc
     8341 aacctgggga acaacgctga aaacctcgac aaccaaaacg agggcttcgc cgcattcggc
     8401 agggtcgtcg gcgctggcat ggacctggtg gacgacatcg ccggatttcc catcggcacc
     8461 taccggaatt tcaacgccgc cggcggcacc aatgccgccc tcgacaagcg cgtcaacctt
     8521 gtggacttca gcgagtccgg ccagcctttc ttgtctggca aggaggccct cgacgccgtg
     8581 cccatgaaca cagaggctga aaacgccccg ctcgacatgg atgtgaacca gaccgtgcgc
     8641 atcctccagg ccgcgcagat ctcccctctc ggctacagcg ttcctgccaa caccgccccg
     8701 gatgtcgtct ccgcctttgt cagcggtggc aatctcgtca tccagggcct caaggagggc
     8761 gtgtccagca tcaccgtcca ggccgccgat ctcgacggcc acctcgtcac ccagaccttc
     8821 accgtcacca tcgtgcgcaa ctacaaggcg cccgtcatca ccaagcagcc tgtctccgtc
     8881 gccgtgctgc ccggcgcgga cgccaagctc gccgtcaccg ccacaggcac cggcccgctc
     8941 aactaccaat ggatgaagga cggccaggac ataaatggtg agaactccgc aaaactgacc
     9001 gtcgccgccg tggatgcagg caagaccggt ttttacgccg tccgcgtctg gaacaactcc
     9061 ctcaccgtgc tgagcaacgt cgtccgcgtg gacctgcgcg cgccacccgt cttcaccacc
     9121 caccccgtgg cgaaggtcgt cgaggccgga aaaccactgg agctggaggt taacggcacc
     9181 ggcgcgcccg tgcccgccat ctcctggctg cgctccggca aggtcgtggc caagcagacc
     9241 tccaccaagc tcagcatccc ctcagccaag atcgccgacg gcggcgttta caccgcccgc
     9301 gccagcaatg tcgtggccaa aaacgtcagc agtcagccgg ctgaggttct cgtcgtggac
     9361 aaaggcgcca agctcgcctt cgcccctgcc gggcggaagc tggcgctcca ggcccaggcc
     9421 gccgccggcc ccggcctact tcatcagtgg ctcaggaacg gtggactctt gctcaccgac
     9481 accgacaatg tcaccggcgt ccagggtccc gtgcttacca tcaaagccct gggctttggc
     9541 gacctcggca gctacacctg ccaggtgctc agtgccgatg gcagcctcac cgccgaaacc
     9601 ggcccgtgga aagtcagcat cccccaaaag cccgtcctgc aaaacttcac cgccgccacc
     9661 gcctacgtcg gcctggacta cgagttcacc cccgcctttg gcggagacgc caacaacagc
     9721 gtcgcctcct tctccatcaa gggactgccc ggcggcctca agtatgacac cgccaccgcc
     9781 cgcatcttcg gcaggcccac ccaccccggc gtcttcaccc tcaccgtcac cggcaagaac
     9841 cccgccggca ccagcgcgcc cgtcaccggc atgctcatcg tcacccccat gcctgagccc
     9901 gccgtaggca cctttgtcgg ccaggtcgcc gcctccagca ccctcaacgg cggcaaagga
     9961 gggcgcatgg acttcaccgt caccgacggc ggcctggtca gcggcaagct caccgtcggc
    10021 aaagacgcgt tcagcttcaa aggcaccatg cgccagacac ccggcagctc cctggccaca
    10081 ggcgaggccg tcatcgcgcg caaaggcggc gccctgcctg tcaccttcac cttttccatc
    10141 ttcgccctcc agggcttcgc cgactccggc aacttcgagg gcactctcag cgacggagtc
    10201 accagcgccc agaccgtcgg ctaccgcaaa gtcttccacg ccacctggaa tccctacagc
    10261 ctgccccaga gctacaacct gggcctcaac cttgccaatg tggaggatgt ggacaaggac
    10321 gccatccccc agggctccgg cttcctacgc gtcgtcacca acaccagtgg cgatgccagc
    10381 atcaccggac gcctggctga cggcaccact ctcacctgca gctccccgct cggcggcgtg
    10441 ctggcaggag ggcaaaacat ccgatttctc gtctatcagt ccctttacaa gcacaccggc
    10501 gccatcgttg gcgaacccgg catcctcgtc ctccagctcg cccccgccgg cgtcaatgac
    10561 atctggttcc gcgtctccgg ccagctccgc tggaccaggg acgcgcagac cgccgccgcc
    10621 gagcgcgctt acaagcccgg atttggaccc gtcatcctcg acgcactcgg catgacctat
    10681 accccgcccg ccagtggcag ccctgtcttc cgcctgccca agtccggcga ggacaatgcc
    10741 cgcatggagt tcgagcacgg cggcatcgag cacgccgcca ccgacccgga cgtgcccctg
    10801 ctcaccatca ccaatgccaa tggcgtctcc atcgccgccg ccaacaatcc cggcggcgtc
    10861 aagctcacca tcacccccgg caccggtctg ctctcaggca gcttcaccgt cgaggacgat
    10921 cccgccgtca ccccgaagcg cggcgccacc ttccacggca tcatcgtgcc gcagatccct
    10981 gacttccaga tccagcagac cgaccagatc ggcaacacca cccctgtcga agtgccaggt
    11041 tcccccgccc gcggcatcgg ccacttcctc ctggcccaga aaccctccgc cggcccgccc
    11101 gccaccaccg ccaagacctc ccccatcctc tccggcatct tccgcctcct ccccgcccca
    11161 atcaccatca acacgcagcc ccagggtgcc accaaggacc ccggtgagaa ccataccttc
    11221 actgtcgccg tcaccgcccc cggcggcacc gtcacctacc aatggcggaa aaacggcctc
    11281 agcatcaccg gcgccaccaa tgccacgctc gtcctcaccg gcatcagcga gggcaacgag
    11341 ggcacctacg atgtcgtcat cagcaccgag tacagccgtg tcgtcagcga ccccgccgtg
    11401 ctgaacgtca acaacatcat ctccgaggtc atcatcaccc gcagcccctc cgacgaggtg
    11461 ctcgacgagg gcggcagcgt caccttcacc gccgtggcca atggcagcgg caccctgtcc
    11521 taccaatggc gcaaggacgg cgacgacatt gagggcgcca ccagttccac ctacatcatt
    11581 gatgaactca tggccgtcca ctccggccag tactccgtgc gcgtcaccag tgccctgacc
    11641 cccggcggct ttgtcagcaa tgtcctcgac ctccaggtcg ccaccggcgt cgtcaatccc
    11701 ctgctcgccc gcctgcctgc caccacggat gtctccattg gctcctccgt caccttcacc
    11761 gtcaccccgg acgggcgccc tccgcacttc ttcacctggt tcaaagacgg cgagctcatt
    11821 gagggcgcca gcaacgcccc caactacatc attcacttcg tcacccagga atcggccggc
    11881 aactattccg tgctcgtctc caacgccctc actcccgagg gtgtctatac caacaccgtt
    11941 cccctcggcg tggatgacaa ggtctccaac gtcgtcgcca cccgcgcccc ggacgaagag
    12001 gccgtcgccg agagcactgc cgtcgaattc accgtcacgc actcgggtgc cggccccttc
    12061 acctaccagt ggtataagaa cgatgtcccc atagacaacg ccacaggcat cagccactcc
    12121 ctgcccgccg ctctcgaggc cgacagcggc aactaccgcg tccgcgtctt caatgcctcc
    12181 accccggaag gagtgctcag tgatcccgtt ccgctcgtcg tcgcacagcc cgtcagcagt
    12241 gtcagcgccg ccctcaccct ccccctcgaa ggccccatcg tcgaaggcgg caccttccgc
    12301 atcaccgcca ccgccaacgg ttccgagccg cgcacctacc agtggtacag aggcggagtt
    12361 cccatagacg gtgccaccgg cagccagctc accatcagcg gcgccacccc cgaagatcac
    12421 gacggcgaat acgctgtcag tgtcagcaac cccgtcacca acgaggagtt cagcaacaac
    12481 gtcgccatcc aggtccagcc cgaaccgtga ccccgcccgc acccaccgcg tcaccgaagc
    12541 cgggatgaac gccgctgaag ctgcgtacgc ctttgccttg cggggcctgc cagtgctttg
    12601 acaacgcgcc gttgttcctt cgcagcacgc cgccgcgctt tgacagtgac aacccgcgta
    12661 aggctgcgcc tggcgtggcg tgcgctgaga tggagacgct gcgctggggc caggcatgga
    12721 ggagtttgtc ctcccggtga ttgtgactct ggatgagccg ggcaagctcg cggaggagcc
    12781 gggctcgccg gagatccgcg agcaccgcat atacttctgg gacaagggca tcgagacccc
    12841 gtcctctgac tgtaaacctg cgcagatcgg gacttccaac tcaagccctt cgacgagcag
    12901 gagcagaaca acgatgaaga tgacgagggc gagggcgggg tgaagaccgc cctgtcaccg
    12961 gacccgccgc aggatttcct ggacgacccc tatgcccatc cgctggcgga cagctacagg
    13021 ttctttgcca atctgacgct ggagagcttc ttcgcccacg ggaacaacct cgccacggtg
    13081 atgcaggtct tgaagcggga gccggatgag tactaccccg cgccggagcg gccatagcag
    13141 gccggcctgg agagccaccc gaggaatggc agcccgccgc ttcggcgtca accccggcgc
    13201 aaatccagag tgagggggaa ctcgggcacc gggggcactg cgacgggctg cattttcccc
    13261 ggtgtgtggc cgccggggaa gaagcgcgcc aggcggcggc tctcggcctc gtagctgttc
    13321 acggggaagg tgtcgtagct gcgcccgccg gggtgggcca catggcaggt gcagccggcg
    13381 atgctgcgct ggttccaggt gtccacgatg tcgaaaacga gcgggctgtg gacgccgatg
    13441 gtgggttgca ggcagttcgg cggctgccag gcgcggtagc ggacgccggc gacgtattcg
    13501 ccgacggtgc cggtggggtg cagagggacg atgcgcccgt tgcaggtgat gaggtggcgg
    13561 ttgttgacga ggccggcggt tttgacctgg atgcgctcca gactgctgtc cacatagcgg
    13621 acggtgccgc cgggggcgcc gtcttcaccg agcacgtgcc agggctcgag ggcggtgcgc
    13681 agatccagat gcacgccgcg gccggtgatg ctgccgacgg tggggaattt gaactcaaaa
    13741 tgcggggcga accagtcggc ggagaggggg tagccaaagt cggccatgtc ttcgatgaca
    13801 tcgctgaagt cctgccagac gaagtgggga aggaggaagc ggtcgtgcag ctcggtgccc
    13861 cagcggacga gcggcgcctc gtagggctgc tgccagaagc gggcgagcgc ggcgcgcagc
    13921 agcaggtgct gggccaggct catctcggcg tgcgggggca tctcgaagga gcgcagctcg
    13981 acgaggccga gccgcccggt gctgctgccg gggtcaaaga gcttgtctat gcagaactca
    14041 ctgcggtggg tgttgccggt ggcgtccacg aggaggttcc gaaagatgcg gtccacgagc
    14101 cagggcgggg tgaagccgcc ggtggcggtc tggcggtcga gctctttgaa ggcgagctcg
    14161 agctcgaaga gggcgtcatt gcgcgcttca tccacgcgcg ggtgctggct ggtggggccg
    14221 atgaagaggc cgctgaagag gtagctgagg ctggggtggt tgtgccagta gccgatgagg
    14281 gattttaata aatcgggccg gcgcaggaag ggggagtggc gcggactctc gccgcccacc
    14341 acgatgtggt tgccgccgcc ggtgccggtg tggcggccgt ccaccatgaa tttttcggtg
    14401 ccgaggcggg tgaggcgcgc ttcctcataa agggcggtgg tgttttcgac gaggtctccc
    14461 caggttttga cggggtgcat gttcacctcg atgacgccgg ggtcgggggt gactttgatg
    14521 acatggagcc tcgggtcata aggcggggat gtgccttcga tgacgatggg gatggcgagg
    14581 gtggcggcca cgtcttcgat ggcggagatg aggtcgaggt agtcctcggt ggcggccacg
    14641 ggcggcatga agacatgcag gcggccctcg cgcggctcga agcagagggc ggtgcggatg
    14701 atccaggagg cggactcttg caggcggggg aaacgggtgc ctgtggggct ggaagggtca
    14761 ttttcttctg gctccagcgg gggcagcttc cggcgctcgg gcttgccctg gccggcgtgg
    14821 atgccctgac gccaggaggc gg
//
LOCUS       DAOMYJ010000124        14703 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Prosthecobacter sp. isolate AMR_MDS_5629
            NODE_1392_length_14703_cov_20.071068, whole genome shotgun
            sequence.
ACCESSION   DAOMYJ010000124 DAOMYJ010000000
VERSION     DAOMYJ010000124.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867850
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prosthecobacter sp. (wastewater metagenome)
  ORGANISM  Prosthecobacter sp.
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Verrucomicrobiaceae; Prosthecobacter.
REFERENCE   1  (bases 1 to 14703)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14703)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.30
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:34:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,742
            CDSs (total)                      :: 4,684
            Genes (coding)                    :: 4,676
            CDSs (with protein)               :: 4,676
            Genes (RNA)                       :: 58
            tRNAs                             :: 55
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14703
                     /organism="Prosthecobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1392_length_14703_cov_20.071068"
                     /isolate="AMR_MDS_5629"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1965333"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..229)
                     /locus_tag="PLP58_13345"
     CDS             complement(<1..229)
                     /locus_tag="PLP58_13345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ09556.1"
                     /translation="MNAGRKRWVFKGGLAKQRADTILTIQSDFARTRSVCLILLPGAT
                     NPSENGLSVLPKALQARQQRVAQLVGAAVALI"
     gene            complement(290..1207)
                     /locus_tag="PLP58_13350"
     CDS             complement(290..1207)
                     /locus_tag="PLP58_13350"
                     /inference="COORDINATES: protein motif:HMM:NF020123.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HRJ09557.1"
                     /translation="MKTRPALLTALVLLTLPLTAQDAPDPRAMMQRMFNPQATKEELL
                     EALKEAGRAGLSRQKVIEAKLIWGLRRQDADWLVRMLPELEILAKNFDPQDSAGLKSA
                     DDVRGFMEYAKALQALEKKDDAAFKTHILEAAWSAPGQAQLFFQLVEKTRREAKMANL
                     KMDLGLVLTTSSGEATTLKDQLAGKKALLIDFWASWCGPCMQLMPALKKKAAELAPHG
                     IVVMGMNKDDENAESVAEKIRDEQGIEFPWLVEPRERPFTQLLEIESIPRMVLVAPDG
                     AILFNGHPQDPALYDALQKLDPAVKKPEA"
     gene            1312..2241
                     /gene="miaA"
                     /locus_tag="PLP58_13355"
     CDS             1312..2241
                     /gene="miaA"
                     /locus_tag="PLP58_13355"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: protein motif:HMM:TIGR00174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0052381 - tRNA dimethylallyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HRJ09558.1"
                     /translation="MPAPFFITGPTASGKSALAVALAERVGGEIVNADAFQLYEGMDL
                     LTAKPSSAEMRRVPHHLYSVLPLTQSCDAQRYRRMALPVIEGISARGLTPIVVGGSGL
                     YIKALSHGLAELPAADPALRARLAALSHEEKRAQLLRLDPDAGVNVPLANPRYVDRAL
                     EVCLLTGQPQSRLRRTFDATPPAGAGVSLVWPRPVLHARIEQRARQMLEAGLLEEVRH
                     LPPTGLTSDKAIGLREMRAHLAGEMPLDEAAALMTQATRQYAKRQLTWFRRESWLQSV
                     CLPADAAAESALALILHHFPCPLPPQQPPSTSA"
     gene            2199..3302
                     /gene="aroB"
                     /locus_tag="PLP58_13360"
     CDS             2199..3302
                     /gene="aroB"
                     /locus_tag="PLP58_13360"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009963591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003856 - 3-dehydroquinate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HRJ09559.1"
                     /translation="MSASTPAATVHVSLGARSYDVEVGRGLLSRIGVVAREKFPGSAR
                     TAVITDSNVAPLYAEAVLASLRAAGITPRLITVPAGETSKSLPHAETVCAEMARSGLD
                     RKSFAIALGGGVIGDLGGFCASIYQRGIPYIQMPTTVLSQVDSSVGGKTGVNLPDAKN
                     MVGNFHQPAHVIADVDTLSSLPRREWNEGFAEIIKHACIRDAAMLPAIEQAGRGGGEL
                     VELIRRNIAIKARIVEADEHETTGLRALLNFGHTLGHAIEAAAGYGGMLHGEAIALGL
                     RAALYLSTRLSGLSESEAARVLDLLRLYQLPLVLEKTFPPEEILRITRMDKKFESGKI
                     RFVLLKQLGEAFVSSEVTEAHLLEALEELDSAL"
     gene            3360..5084
                     /gene="araD"
                     /locus_tag="PLP58_13365"
     CDS             3360..5084
                     /gene="araD"
                     /locus_tag="PLP58_13365"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009961833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinonate dehydratase"
                     /protein_id="HRJ09560.1"
                     /translation="MTRKTSEQLRSYRWYGRDELRAFGHRSRAKQAGWGAEDYAGKPV
                     IGILNSWSEQNSCHMHLKLTADAVRRGILQAGGHPMEIPVLSAGEQLTKPTAMFHRNL
                     LAMETEEVLRANPLDAAVLLGGCDKSTPGLLMGAFSMDIPVIYMPCGPMIKGNWRGET
                     LGSGSDVWKYWDEKRAGNLTWEEWCEIEDGIARSPGHCMTMGTASTLTALAETLGLSI
                     PGASSIPAVDSAHLRIAAAAGRQIVENAWLDLKPSALVTERSFENAIAVDMALGGSTN
                     AIVHLIAMAGRLGIPLPLEKFDEISRRVPLLANLRPAGKYLMEEFYHAGGIKALLHVM
                     RDLLHMDERTITGLTLGENVAGCEIHDTDVIREPDNALQPDGGTAILRGNLCPNGAVI
                     KHAAATPALCQHTGPALVFDSYPQMKAAIEDMDLDVTPDTVLILRNAGPVGAPGMPEW
                     GQLPIPKKLILQGVRDMVRLSDSRMSGTSYGACVLHIAPESAVGGPLALVQNGDLIEL
                     DVAARRLHLHVSDEELAKRRARWQPAPPRYHRGYARLFVEHVTQADEGADFDFLQHGP
                     PVPEPEIF"
     gene            complement(5229..7058)
                     /locus_tag="PLP58_13370"
     CDS             complement(5229..7058)
                     /locus_tag="PLP58_13370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ09561.1"
                     /translation="MQPDIIIIKALQSILKAGKSAVIVALMASQTWGAGGLDLTPPLP
                     GLGGGTFSPQGGFQLTAGQIAVIRQRASFRPDFSPQARNSRPTLVLLIHGRTARPGDE
                     PGVFDPPLPQTGLSNRPGTVGYSRFYWDFPFVSAMLGGAGSLFTMDGGGANRLMGPAA
                     WKLARIENDRISNQFAFGADPAPLRGRFTGTAAALVRADGSIALGQMAGQVLREIRAL
                     RDAFAAYAGREPYVVLAGHSKGGLVIRYLLSNPEGQVAGYRLSPEERLFIEELRNDTR
                     FAMTISTPHTGSPVADVFQEVRAAIDNAEGFANQVVEAWNQTRSVAALLGITLPEAVP
                     VDFSAAKEAISNGEDDLGHLSTAFVNGLNNGPLHPKNMARTDGTRIPLYLYGGRSPGG
                     VFFQLGRTDGADVRDFAGRLESSIPDIRVAAASAGGLMGLDYVIHNGVFMDWGRILNG
                     GTGAKPLDLVRRSYVVYFPGMRPRMSQPGERPLSLFEGVPTYYLRNAADQETDNDGMV
                     GIDSALGIGLFSGPVAIEERLARRQVVNAEMMEPFDHTIMRDENGRIDQRGGWYRMYS
                     GAWNFTNHFDIMKRPELGIEARRLLSGAGPHAGAGALSVWPAR"
     gene            complement(7304..8512)
                     /gene="purT"
                     /locus_tag="PLP58_13375"
     CDS             complement(7304..8512)
                     /gene="purT"
                     /locus_tag="PLP58_13375"
                     /EC_number="2.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018969920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate-dependent phosphoribosylglycinamide
                     formyltransferase"
                     /protein_id="HRJ09562.1"
                     /translation="MPRLVMMGGMSRIGTPLSSSATKAMLLGSGELGKEVVIELQRLG
                     VEVIAVDRYANAPAMQVAHRSHVISMLDGEALRRLVEVEKPGLIIPEIEAIATGTLVE
                     LEAEGFTVVPTARAAQLTMNREGIRRLAAEELGLKTSPYIFAQTEEEFRAAIEKIGMP
                     CVVKPIMSSSGKGQSVVKTAADIKPSWKYAQEGGRAGKGKVIVEGFVEFDYEITMLTV
                     RHAGGTSFCAPVGHTQIKGDYRESWQPHPMSHKALESAQEMAGKITGALGGRGLFGVE
                     LFIKGDDVIFSEVSPRPHDTGLVTLISQDLSEFALHVRAILGLPIPNIHQHGPSASCA
                     VLVEGESSMVQFGALDQVLAEPDTQLRLFGKPSVSGQRRMAVTLARAENINTARAKAH
                     RAATAMEIRL"
     gene            complement(8540..9169)
                     /locus_tag="PLP58_13380"
     CDS             complement(8540..9169)
                     /locus_tag="PLP58_13380"
                     /inference="COORDINATES: protein motif:HMM:NF020893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HRJ09563.1"
                     /translation="MRHSLTLLVILLLAGIILPFLIWGEAFESMLSLEGARAWMENYG
                     SWAWLAGVALLVADIVLPIPGTVVMSALGWKYGWLLGGCIAAAGSMLSGAAAYFLCRR
                     LGHGAAVWIAGEEGLKRGGVLFERHGGWLVALSRWTPVLPEAVACLAGLCGMRRRVFF
                     PALACGSLPLGFAFAAIGHMGHESPALALTLSAVAPAALWLAALRRLRK"
     gene            complement(9169..10422)
                     /locus_tag="PLP58_13385"
     CDS             complement(9169..10422)
                     /locus_tag="PLP58_13385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018970280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence/damage-inducible protein A"
                     /protein_id="HRJ09564.1"
                     /translation="MRIELVNTGTELCIGDVINTNAAWIGQRMAALGLEVARQTIVPD
                     GGAVREAIAEAAARSDVVLVSGGLGPTNDDVTRESAADLLGLELRQDAAVTSHLDAYF
                     SKRGKPVTSATLRQAMVPAGARVLMNPFGTAPGLYFPAHLGAARGWKAHLFLLPGPPR
                     ELKPMVENEVEPRLRALLPDGESRRVLYLRLTGIGESDIVEAVEKQLEAVPRLELGYC
                     IGKGDVDVRLAGPQETVDAGARIVRERLGEFILSEDRRVIEEVVVHALAARGEWLATA
                     ESCTGGAIANRVTDVSGSSAVFGHGLVTYANEAKQKHLGVPADLIETHGAVSAEVARA
                     MAEGCLAAAGADHALAVTGIAGPAGGTEEKPVGTVWVALASKGCETAARRFFYPGARD
                     RFKLLTSQAALEMLRRRLYGLPLPG"
     gene            complement(10501..10926)
                     /gene="ndk"
                     /locus_tag="PLP58_13390"
     CDS             complement(10501..10926)
                     /gene="ndk"
                     /locus_tag="PLP58_13390"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009959563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004550 - nucleoside diphosphate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0006165 - nucleoside diphosphate
                     phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HRJ09565.1"
                     /translation="MAQETSLLLLKPDCVAQGLNGEVLKRLEAEGFRVRGIKMIQLTD
                     ELLKEHYAHIADKPFFPDVAGFMKSKPVIAVALGGENVISHVRDLLGPTDSKAAPKGT
                     IRGDFGSDKMTNVVHASDSPEAAAIELRRFFKDGEIFNY"
     gene            11117..12607
                     /locus_tag="PLP58_13395"
     CDS             11117..12607
                     /locus_tag="PLP58_13395"
                     /inference="COORDINATES: protein motif:HMM:NF021351.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GldG family protein"
                     /protein_id="HRJ09566.1"
                     /translation="MPGTDTEPAATETPDMPRRWGIGLNVLLQVLLAAAIFAGVNRLS
                     YRHHTRWDLSPQQSYTLSPVTLNYLSRLPKDVFIANVFARDGKLYGDVEALLESYRAN
                     GRGRIRVRSIDPLRDIERAEELKADTGLALDQNGVVIRSGSRTRFIREEEMVVRGPGT
                     EAVRPVSEFRGEDAVTSAIINVVEGEQRKFFVIVGKGARTEAALVDAMTAIGELGRQQ
                     NFQLLPLNLAEVSRVPEDADGVVLAGIRYDFTAREMAMLKDYWEGRRAGLLVLLDPEA
                     ETPRLNAFLGMHGVRPRADRVLYAESTSTGRRKEFSVRAVFDNESPITLPLSTSNTTL
                     PGQTQSLDLTFDDPYLQNQNIVVHPLIGASERYWGERNYLEEMPVADEADTREDIFLA
                     ASVERGSVLEESLRVDTCRMVVVANSALLDQKTALGVNRDFVSASLNWLINRDRLMGI
                     TPKVKKNYRIQLTPRQGELIFWMTTCVFPALVLVLGLMVWASRRAS"
     gene            12617..14431
                     /locus_tag="PLP58_13400"
     CDS             12617..14431
                     /locus_tag="PLP58_13400"
                     /inference="COORDINATES: protein motif:HMM:NF025598.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4340 domain-containing protein"
                     /protein_id="HRJ09567.1"
                     /translation="MNPRSTLLLFLLAAALGAVILGVEKYLPSTLELREMKRGPVKFD
                     TRLVTAIEIESGGDGISLALRDGRWRVRRPFDDLADPERVLKLLAEIPAIGWIQRVRQ
                     DEFDGDGWAKTSLDKPRHHIRLLAGEREILDLAVGAVSPVEGAHYLSVRQPGRTDGTA
                     SYVSRTTLPDLLKTKPEDWRDAKLLRLAAAKVISLKLGQNGGQIELARAGDQRPWALV
                     KPLRARVGKEKVDGLLSTLLNLEITGVLDAPPAPAGEDTAGSSLKITASTSGPPEEVF
                     EITLTRPGEDAAQTRAVCGHRGFVFTVRSDTLKDLWSQPNDLRDRMLARIDGQNVSAI
                     RIESLLFPEVGLEKKDGSWYLSRHQKWEPANGERVLRFFEALNTHDILEFTADSAANL
                     VPYGLDKPFLKVRWTESGAEHSLLFGKDAEGAAFFAKHGDEPSIYRIEASLLPSIPQD
                     SIKWKGLGALRFSQFALRRITLAAGSAPPTVLDYDPSTAQWTGQRAGRDITGMIDRVK
                     ADTLAGKLAKFTVQDWSADITAAIHALRQPAVQVRVTLGEPGVSTGPTRETLLKFAPT
                     QEGMDTALWFGQVDNGPDVFYITRTALMDLLAPVFKPE"
BASE COUNT         2552 a         5311 c         4442 g         2398 t
ORIGIN      
        1 caatcaatgc caccgcagca ccgacaagct gcgcgacgcg ctgctgccgg gcttgaagcg
       61 cctttggcag aacgctcaag ccgttctcag atgggttggt cgcccctggc agcaggatca
      121 aacaaacgct tcgtgtccgc gcaaaatccg attgaatcgt caggatggta tcagcccttt
      181 gtttcgcaag cccacccttg aagacccagc gtttccttcc ggcattcatt gcctgggtcg
      241 ctctgctgaa tcagatgccg ctcgcccatt gagcaggatt caatacccgt cacgcctccg
      301 gcttcttgac cgcaggatcc agcttttgca gcgcgtcata aagcgcggga tcctgcggat
      361 gaccgttgaa gaggatggct ccatccggcg ccacgagcac catgcgcggg atgctttcga
      421 tctccaaaag ctgtgtgaag gggcgctcgc gcggctcgac gagccaggga aactcgatgc
      481 cctgctcatc gcggattttc tcggccacgg actcggcgtt ctcgtcgtcc ttgttcatgc
      541 ccatgaccac gatgccgtgc ggagcgagct cggcggcttt ctttttcagc gcgggcatga
      601 gctgcatgca cgggccgcac caggaggccc agaagtcaat gagcagcgct tttttgccgg
      661 caagctggtc cttcagcgtg gtggcctcgc ccgaggacgt ggtgagcacc aggccgaggt
      721 ccattttcag attcgccatc ttcgcctcgc ggcgggtttt ttccacgagc tggaaaaaga
      781 gctgcgcctg ccctggagcc gaccacgccg cctcaaggat gtgggttttg aaggcggcat
      841 cgtctttttt ctccagcgcc tgcagcgcct tcgcatactc catgaagccg cgcacatcgt
      901 cggcggactt cagcccggcg ctgtcttgcg ggtcgaaatt cttcgccaga atctccagct
      961 cagggagcat cctcaccagc cagtccgcat cctgccggcg caggccccag atgagcttgg
     1021 cctcaatgac cttctgcctt gagaggcccg cgcggcccgc ctctttgagc gcctccagca
     1081 gttcctcttt cgtggcctgc ggattgaaca tgcgctgcat catcgcccgt ggatcaggcg
     1141 cgtcctgggc ggtgagtggc agggtgagga ggacgagtgc ggtcagcagg gcggggcggg
     1201 ttttcatggc gaaaaaatca aaacgtgcgc ggcggcgtca tgttgccctc cttccgcgcc
     1261 ttggctagtg atgaaatgcc acgccccgcg cgcacccccg ccacgcaaac catgcccgcc
     1321 ccctttttca tcaccggccc caccgccagc ggaaaatccg ccctggccgt ggcactcgcc
     1381 gagcgcgtcg ggggggagat cgtgaacgcg gatgccttcc agctctatga aggcatggac
     1441 ctgctcaccg ccaagccctc cagcgccgaa atgcgccgcg tgccgcacca tctctacagc
     1501 gtgctgccgc tcacgcagag ctgtgatgcc cagcgctacc gccggatggc cctgcccgtg
     1561 atcgagggca tctccgcgcg cggcctcacg cccatcgtcg tcggcggctc cggcctttac
     1621 atcaaggccc tctcccacgg cctcgccgag ctgccagccg cagaccccgc cctgcgcgcc
     1681 cgtctggccg ccctcagcca tgaggaaaaa cgcgcgcaac tcctgcggct cgaccccgat
     1741 gccggggtca acgtgccgct cgccaatccc cgctatgtgg accgcgccct ggaagtctgc
     1801 ctgctcaccg gccagcccca gtcccggctg cggcgcacct ttgacgccac accacccgca
     1861 ggcgccggcg tcagcctcgt gtggccgcgc ccagtgctgc acgcgcgcat cgagcagcgc
     1921 gcgcgccaga tgctggaggc cggcctgctg gaggaagtcc gccatctgcc acccaccggc
     1981 ctcacttcgg acaaagccat cggcctgcgt gagatgcgcg cgcatctggc cggtgagatg
     2041 cctttggatg aagccgccgc gctcatgacc caggccaccc gccagtatgc gaaacgccag
     2101 ctcacctggt tccgccgtga gtcctggctg caaagcgttt gcctgcccgc cgatgccgcc
     2161 gcagaatccg cgctcgccct catcctgcac catttcccat gtccgcttcc accccagcag
     2221 ccaccgtcca cgtcagctta ggcgcccgca gttacgatgt ggaggtcggg cgcgggctgc
     2281 tcagccgcat cggcgtggtg gccagggaaa aattccccgg cagcgccagg accgccgtca
     2341 tcaccgactc gaatgtcgcc ccgctttatg ccgaggccgt gctcgccagc ctgcgcgccg
     2401 cgggcatcac cccgcgcctc atcaccgtgc cggcgggtga gaccagcaaa tccctcccgc
     2461 acgccgagac cgtctgcgcg gagatggcgc gctccggcct ggaccgcaaa agcttcgcca
     2521 tcgccctcgg cggcggcgtc atcggcgacc tcggcggctt ttgcgccagc atctaccagc
     2581 gcggcatccc ctacatccag atgcccacca ccgtgctctc ccaggtggac agctccgtgg
     2641 gcggcaagac cggggtgaac ctgcccgacg ccaaaaacat ggtcggcaac ttccaccagc
     2701 cggcccatgt catcgccgat gtggacaccc tctccagcct gccccggcgc gaatggaacg
     2761 agggcttcgc cgagatcatc aagcacgcct gcatccgcga tgccgccatg ctgcccgcca
     2821 ttgagcaggc cggccggggc ggcggcgaac tcgtggagct catccggcgg aacatcgcca
     2881 tcaaagcccg catcgtcgaa gccgacgagc acgagaccac cggcctgcgc gccctgctga
     2941 acttcggcca caccctcggc cacgccatcg aggccgccgc cggttatggc ggcatgctgc
     3001 atggcgaggc catcgccctc ggccttcgcg ccgcccttta cctctccacc cgtctgagcg
     3061 gcctgtcgga aagtgaagcc gcccgcgtgc tcgacctcct gcgcctctat cagttgccct
     3121 tggtcttgga aaaaaccttc ccgccggagg aaatcctgcg catcacccgc atggacaaaa
     3181 aattcgagtc cggcaaaatc cgcttcgtgc tcttgaagca gctcggcgag gcctttgtca
     3241 gcagcgaagt caccgaggcg cacctgctgg aagccctgga ggagctcgac tcagcccttt
     3301 gagaaaaccc gcgccctttg cctgacccgg atcaacccct ccgccaccct ccacttatca
     3361 tgacccgcaa gacctccgaa caactccgct cctaccgctg gtatggccgt gacgaactcc
     3421 gcgccttcgg ccaccgctcc cgtgccaagc aggccggctg gggggcggag gattatgcgg
     3481 gcaagcccgt catcggcatc ctcaactcct ggtccgagca gaactcctgc cacatgcacc
     3541 tgaagctcac cgccgacgcc gtgcgccgcg gcatcctcca ggccggcggg cacccgatgg
     3601 agatccccgt gctctccgcc ggcgagcagc tcaccaagcc caccgccatg tttcaccgca
     3661 acctcctcgc catggagacc gaggaagtgc tgcgcgccaa cccgctcgac gccgccgtgc
     3721 tgctcggcgg gtgcgacaag tccacccccg gcctgctcat gggcgccttc agcatggaca
     3781 tccccgtcat ttacatgccc tgcggcccca tgatcaaagg caactggcgc ggcgagaccc
     3841 tcggctccgg cagcgatgtc tggaagtact gggatgaaaa acgcgccggc aacctcacct
     3901 gggaggaatg gtgcgagatc gaggacggca tcgcccgcag ccccggccac tgcatgacca
     3961 tgggcaccgc ctccaccctc accgccctcg ccgagaccct cggcctcagc atccccggtg
     4021 catcctccat ccctgctgtg gacagcgccc acctgcgcat cgccgccgcc gctggccgcc
     4081 agatcgtgga aaatgcctgg ctcgatctca agccctcagc ccttgtcacc gagcgctcct
     4141 ttgaaaacgc catcgccgtg gacatggccc tgggcggcag caccaacgcc atcgtccacc
     4201 tcatcgccat ggcaggccgc ctcggcatcc cgctgccctt ggagaaattc gacgaaatct
     4261 cccgccgtgt gccactgctc gcgaatctgc gccccgccgg caaatacctc atggaggagt
     4321 tttatcatgc gggcggcatc aaagccctgc ttcacgtcat gagggatctc ctccacatgg
     4381 acgagcgcac catcaccggc ctcacgcttg gtgaaaacgt cgccggctgc gagatacatg
     4441 acacagacgt gatccgcgag ccggacaacg ccctccagcc cgacggcggc accgccatcc
     4501 tgcgcggcaa cctctgcccg aacggagccg tcatcaaaca cgccgccgcc acacccgccc
     4561 tctgccagca caccggcccc gcgctcgtgt tcgacagcta cccgcagatg aaggccgcca
     4621 ttgaggacat ggacctcgac gtcaccccgg acaccgtgct catcctgcgc aatgccggcc
     4681 ctgtgggcgc gcccggcatg cccgagtggg gccagctccc catcccgaaa aaactcatcc
     4741 tccaaggcgt gcgcgacatg gtgcgcctca gtgacagccg catgtcaggc accagctacg
     4801 gcgcatgcgt gctccacatc gcccccgaga gcgccgtcgg cggccccctg gccctggtcc
     4861 aaaatggcga cctcatcgag ctcgacgtcg ccgcccgccg cctccacctc catgtcagcg
     4921 atgaggaact ggccaaacgc cgcgcccgct ggcagccagc tccgccgcgc tatcaccggg
     4981 gttatgccag gctcttcgtc gagcacgtca cccaggccga tgaaggcgcc gacttcgact
     5041 tcctccagca cggcccgcct gtgcccgagc cggagatctt ttgagaccaa ccgccaagaa
     5101 tctttcggcc ttgcgactga agtagaacaa aaatgcgtct ccagacatgg agcagccaag
     5161 cactgtcact tctgacaaaa gccccgtggc cgatcagcca cggggcttgc ttctcttcca
     5221 cctctccatc aacgggcagg ccagacactg agagcgcccg cgccggcatg aggtccggca
     5281 ccactcagca ggcggcgcgc ctcaatgcca agttcaggcc gtttcatgat gtcaaaatgg
     5341 ttcgtaaaat tccatgcccc ggaatacatg cgataccagc ccccgcgctg atcaatgcgg
     5401 ccattttcat ccctcatgat ggtgtggtcg aaaggctcca tcatctccgc attgaccact
     5461 tggcggcggg ccaggcgttc ctcgatggcc acagggccgg aaaacaatcc aatacccagc
     5521 gctgagtcaa ttcccaccat gccgtcgtta tcagtctcct ggtcagcagc attgcgcagg
     5581 tagtaagttg gaactccttc gaaaagtgac aaaggccgct ctccaggctg agacatgcgc
     5641 gggcgcatcc ccggaaagta taccacataa gaacggcgca cgagatccag cggtttggct
     5701 ccggtgccgc cattcaggat gcgaccccag tccatgaaca cgccgttgtg aatcacataa
     5761 tccagcccca tcaaaccgcc tgcggaagcc gccgcgacgc ggatgtcagg gatgctgctc
     5821 tccagacggc cggcaaagtc gcggacatcg gcgccgtccg tgcggccaag ctggaagaaa
     5881 accccgccgg gactgcgtcc gccgtagagg tagaggggga tgcgtgtgcc atctgtccgg
     5941 gccatgtttt ttggatgcag cggaccgttg ttcagcccgt tcacaaaggc ggtgctcagg
     6001 tgtcccagat catcttcccc gttgctgatc gcttccttgg cggcgctgaa atccaccgga
     6061 acagcttcgg gcagggtgat gcccaacaag gcggcgacgg agcgggtctg gttccaggcc
     6121 tccacgacct ggttggcaaa gccctcggca ttgtctatgg ccgcgcgcac ctcctgaaag
     6181 acatccgcca cggggctgcc cgtgtgagga gtgctgatgg tcatcgcaaa gcgcgtgtca
     6241 ttgcgcagct cttcaatgaa aagcctctcc tccgggctga gccggtagcc tgccacctgg
     6301 ccctccgggt tgctcaggag gtaacggatg accagcccgc ccttgctatg ccctgccagc
     6361 accacataag gctcgcgtcc ggcataagcg gcgaacgcat cgcgaagcgc cctgatttcc
     6421 cgcaagacct ggcccgccat ctgtccaagg gcgatggagc cgtccgcacg cacgagcgcg
     6481 gccgccgtgc cggtgaaacg tccgcgcagt ggggcggggt cagccccaaa ggcgaactgg
     6541 ttggaaatgc ggtcgttttc aatccgggcc agcttccatg ccgccggtcc catcaggcgg
     6601 ttcgcgcctc ctccgtccat ggtgaacaaa ctgccggctc cgcccagcat ggcggagaca
     6661 aaggggaaat cccagtaaaa gcgcgagtag ccgaccgtgc ctggacggtt gctgagaccg
     6721 gtctgcggaa gaggtgggtc gaaaacaccg ggctcgtccc caggacgggc agtgcgaccg
     6781 tggatgagca gcacgagagt cgggcgggag ttgcgcgcct gcggactgaa gtccggacga
     6841 aaactggccc gctggcggat aacggcaatt tgtccggctg tgagctggaa gccgccttgt
     6901 ggcgaaaacg tgccgccgcc aaggccggga agcggtggtg tcagatcgag acctccggcc
     6961 ccccaggttt gactggccat gagggcgacg atgacggcgc tcttgccagc tttgaggatg
     7021 gattggagcg ctttgatgat gatgatgtca ggttgcatga tgattcaggg tgtgagtgtt
     7081 ggcgggatcg gaatcgtttg ttccgtgtcc tcatccccct gatgcgggtc gctcagcgaa
     7141 cttgcgcaaa tgctgaaaaa aacctcaatc tgcctcccgt cattcttcgg aagtcacgtg
     7201 acatcccgtc gggggatgtc gttggagagc actttcagac cacgacatgg cggactgtgc
     7261 gcggttgaaa aggtgtggat attttcaggt cagccttgct aactcacagc cggatctcca
     7321 tcgccgtggc ggcccggtgg gctttggcgc gggcggtgtt gatgttttcc gcgcgggcca
     7381 gcgtcacggc catgcggcgc tggccgctga cacttggctt gccgaacaaa cgaagctgcg
     7441 tgtccggctc ggccagcact tgatcgagcg cgccgaactg caccatgctc gactcgccct
     7501 ccaccaggac cgcgcagctc gcgctggggc cgtgctggtg gatgttcggt atcggcaggc
     7561 cgagaatggc gcgcacatgc agggcgaact cggacaagtc ctgcgagatc agcgtcacca
     7621 gcccggtgtc gtgcggacgc ggggacacct cgctgaagat cacgtcatcg cccttgatga
     7681 aaagctccac gccaaacagc ccgcgcccgc cgagcgcccc ggtgatcttt cccgccatct
     7741 cctgcgccga ctccagcgcc ttgtgcgaca tgggatgcgg ctgccaggac tcgcggtagt
     7801 cgcccttgat ctgggtgtgg cccaccggcg cgcaaaagct ggtgccgccg gcgtggcgca
     7861 cggtgagcat ggtgatctcg taatcaaact cgacgaaacc ctcgacgatg accttcccct
     7921 tccccgcgcg cccgccctcc tgcgcgtatt tccaggatgg cttgatgtcg gcggcggttt
     7981 tcaccacgct ctgccccttg ccgctgctgc tcatgatcgg cttcaccacg cagggcatgc
     8041 cgattttttc aatcgccgcg cggaactcct cctccgtctg cgcgaaaatg tagggggagg
     8101 tcttcaagcc cagctcctcg gcggccaggc ggcggatgcc ctcgcggttc atggtgagct
     8161 gcgcggcgcg cgcggtgggc accacggtga agccctccgc ctccagctcc accagcgtgc
     8221 cggtggcgat ggcctcgatc tccgggatga tgaggccggg cttctccacc tccaccagcc
     8281 ggcgcagcgc ctcgccatcc agcatggaga tcacatggct gcggtgcgcc acctgcatgg
     8341 cgggcgcgtt cgcgtagcgg tccacggcga tgacctcgac gccgaggcgc tgcagttcga
     8401 tgacgacctc cttgcccagc tccccggagc cgaggagcat ggctttggtg gcggaggagg
     8461 aaagcggggt gccgatgcgg gacatgccgc ccatcatgac aaggcgcggc acgcgcgcaa
     8521 gagacgcggc ccggagcgct cactttcgca gccgtctcaa agccgccagc cacagcgccg
     8581 caggcgccac ggcgctgagg gtgagcgcca gcgcggggct ttcatgcccc atgtgcccga
     8641 tggcggcaaa ggcgaagccg agcggcagcg agccgcaggc cagcgcggga aaaaagacgc
     8701 gccgcctcat gccgcacagc cccgccaggc aggccaccgc ctcgggcaaa acaggcgtcc
     8761 agcgcgagag ggccacgagc cagccgccgt gccgttcaaa cagcaccccg ccccttttca
     8821 aaccctcctc ccccgcgatc cacaccgccg ctccatggcc cagacggcgg cagagaaaat
     8881 acgcggcggc ccccgaaagc atggaccccg ccgcggcgat gcagcccccc aggagccagc
     8941 cgtatttcca gcccagcgcg gacatgacca cggtgccggg gatgggcagc acaatgtcgg
     9001 ccaccagcag cgcgacgccc gccagccagg cccaggagcc gtagttctcc atccatgcgc
     9061 gcgcgccctc caggctcagc atggactcaa acgcctcccc ccagatgagg aagggcagga
     9121 tgatcccagc cagcagcagg atgaccagaa gtgtgaggga gtgacgcatc agccgggcag
     9181 cggcagacca tacaggcggc ggcgcagcat ttccagcgcg gcctgggagg tgagcagctt
     9241 gaagcggtcc cgcgcgcctg gataaaagaa gcgccgcgcc gccgtctcgc atcccttcga
     9301 ggccagcgcc acccacaccg tgccgacagg cttttcctcc gtgccgcccg cgggtccggc
     9361 gatgccggtg accgccagcg cgtgatccgc ccccgccgcc gccaggcagc cctcggccat
     9421 ggcgcgcgcc acttccgcgc tcaccgcgcc atgagtttca atgaggtccg ccggcacgcc
     9481 gaggtgcttt tgcttggcct cgttggcata agtcaccaag ccgtgcccga agacagccga
     9541 ggagccgctg acatcggtga cgcggttcgc aatcgccccg ccggtgcagg attcggcggt
     9601 ggccagccac tcgccgcgcg cggccagcgc atgcaccacc acctcctcga tcacacggcg
     9661 gtcctcggac aaaatgaatt cccccagccg ctcgcgcacg atccgcgcgc cggcgtccac
     9721 agtctcttgc ggccccgcca gccgcacatc cacatcgccc ttgccgatgc agtagcccag
     9781 ctccaggcgc ggcacggctt cgagctgttt ttccaccgcc tccacgatgt cggactcgcc
     9841 gatgccggtg agccgcagat acaggacgcg gcggctctcc ccgtcgggca gcagcgcgcg
     9901 caggcgcggc tcgacctcgt tctccaccat cggcttcaac tcccgcggcg ggccgggcag
     9961 cagaaaaagg tgcgccttcc atccgcgcgc cgccccgaga tgagccggga agtaaagccc
    10021 gggcgctgtg ccgaatggat tcatcaacac ccgcgcaccc gcgggcacca tggcctggcg
    10081 cagcgtggcg gaggtcacag gtttgccgcg cttggaaaag taggcgtcca gatggctcgt
    10141 caccgccgcg tcctgccgca gctccagtcc aagcaaatcc gcggcggact cgcgcgtcac
    10201 gtcgtcattg gtcgggccga gcccgccgga aacgagcacg acatcggagc gcgcggcggc
    10261 ctcggcgatg gcctccctca ccgcgccgcc gtcggggacg atggtctggc gcgccacttc
    10321 gagaccgagc gcggccatgc gctgcccgat ccatgcggcg ttcgtgttga tgacatcacc
    10381 gatgcatagc tcggtgccgg tgttgacgag ttcgatgcgc atgggcgtgt gagtttggca
    10441 gggcacgctc gtgccgcctg tccatccgca tgaggaacga gcgcgcggcg cgcggcggca
    10501 tcaatagttg aagatttcgc cgtccttgaa gaagcgcctc agctcgatgg ccgccgcctc
    10561 gggcgagtcg gaggcatgga ccacgttggt cattttgtcc gagccgaaat caccccggat
    10621 ggtgcctttc ggcgcggcct tcgagtccgt ggggccgagc aggtcgcgca catgggagat
    10681 gacattttca ccgccgaggg ccacggcgat gacgggcttg ctcttcatga agccggccac
    10741 gtcggggaag aagggcttgt cggcgatgtg ggcgtagtgc tccttcaaaa gctcgtcggt
    10801 gagctggatc atcttgatgc cgcgcacgcg gaagccctcg gcctccaggc gcttgaggac
    10861 ttcgccattg agtccttggg cgacgcagtc gggcttgagc aggagaaggg aggtttcttg
    10921 agccatgagg ggtgtttttt tatatgggaa aaaaggggcc ggatagtgct gtccgcgccc
    10981 tggagagtca aggtggaaga tggcgcggcg ccgcgtcccg caaagcagcc gaaggaaggc
    11041 gcgcgacttt tgaaaagaga gttcggcggt tgccctccgg gtaggatggg ctttgttggc
    11101 ccctcccatt ttgccaatgc ccggaactga caccgaaccc gccgcaaccg agacgccgga
    11161 catgccccgg cgctggggca tcggcctgaa tgtgctgctg caggtgctgc tggcggccgc
    11221 catcttcgcc ggggtgaacc gcctcagcta ccgccaccac acccgctggg acctcagccc
    11281 gcagcagagc tacaccctca gcccggtgac attgaactac ctctcccggc tgcccaagga
    11341 tgtcttcatc gccaatgtct tcgcccgcga cggcaagctc tacggggatg tcgaggccct
    11401 gctggagtcc taccgggcga acggacgcgg gcgcatccgc gtgcgctcca ttgacccgct
    11461 gcgcgacatc gagcgcgccg aggagctcaa ggccgacacc ggcctcgcgc tcgaccagaa
    11521 cggcgtggtc atccgctccg gcagccgcac ccgcttcatc cgcgaggagg agatggttgt
    11581 gcgcggaccc ggcacggagg ccgtgcggcc cgtgagcgag ttccgcggcg aggacgccgt
    11641 gacctccgcc atcatcaatg tcgtcgaggg cgagcagcgg aaatttttcg tcatcgtcgg
    11701 caagggcgcg cgcaccgagg cggccctggt ggacgccatg accgccatcg gcgagctggg
    11761 ccgccagcag aatttccagc tcctgccgct caacctggcc gaggtcagcc gcgtgcccga
    11821 ggacgccgac ggcgtcgtcc tggcaggcat ccgctatgat ttcaccgcgc gcgagatggc
    11881 catgctcaaa gactactggg agggcaggcg cgcgggcctt ctcgtgctgc tcgaccccga
    11941 ggccgagacc ccgcgcctga acgccttcct cggcatgcat ggcgtgcgcc cgcgcgccga
    12001 ccgcgtcctt tacgccgaga gcaccagcac cggccggcgc aaggaattct ccgtgcgggc
    12061 ggtcttcgac aacgagtcac ccatcactct tccgctttcc acctcgaaca ccaccctgcc
    12121 cggccagacg cagtcccttg atctcacctt cgacgacccc tacctgcaaa atcaaaacat
    12181 cgtcgtccac ccgctcatcg gcgcctccga gcgctactgg ggcgagcgca actacctcga
    12241 ggagatgccc gtcgccgacg aggccgacac gcgcgaggac atcttcctcg ccgcctccgt
    12301 cgagcgcggc agcgtgctgg aggagagcct gcgcgtggac acctgccgca tggtcgtggt
    12361 ggcgaactcc gcgctcctcg accagaaaac cgccctcggc gtgaaccgcg actttgtctc
    12421 cgccagcctg aactggctca tcaaccgcga ccgcctcatg ggcatcaccc ccaaggtcaa
    12481 aaagaactac cgcatccagc tcaccccgcg ccagggcgag ctcatcttct ggatgaccac
    12541 ctgcgtcttt ccagcccttg tcctcgtgct cggcttgatg gtctgggcct cccgccgcgc
    12601 ctcctgaccc gccgccatga acccgcgctc gaccctgctg ctcttcctgc tcgccgccgc
    12661 gctcggcgcc gtcatcctcg gcgtcgaaaa atacctcccc tccaccctcg agctgcggga
    12721 gatgaagcgc gggccggtga aattcgacac gcgcctcgtc actgccatcg agatcgagtc
    12781 cggcggcgac ggcatcagcc tcgccctccg ggacgggcgc tggcgtgtgc gccgcccctt
    12841 tgacgacctg gccgaccccg agcgcgtcct caaactcctg gccgaaatcc ccgccatcgg
    12901 ctggatccag cgcgtgcgcc aggacgagtt cgacggcgac ggctgggcca aaacctccct
    12961 cgacaaaccc cgccaccaca tccgcctcct ggccggggag cgggagatcc tcgacctcgc
    13021 cgtcggcgcc gtctccccgg tcgagggcgc tcattacctc tccgtccgcc agccagggcg
    13081 cacggacggg accgccagct acgtctcccg caccaccctg cccgacctct tgaagacaaa
    13141 gcccgaggac tggcgcgacg ccaagctcct gcgcctggcc gccgccaaag tcatcagtct
    13201 caagctcggc cagaacggcg ggcagatcga gctggcgcgc gccggagacc agcggccctg
    13261 ggcgctggtc aagcccctgc gcgcgcgggt cggcaaggaa aaggtggacg gcctgctctc
    13321 caccctcctc aacctggaga tcaccggcgt gctcgacgcc ccgccagcgc cagccggcga
    13381 ggacaccgcg ggctcgtctt taaagatcac cgcctccacc tccggcccgc cggaggaggt
    13441 ctttgagatc acgctgacca ggccgggtga ggacgccgcc cagacacgcg ccgtctgcgg
    13501 ccaccgcggc ttcgtcttca ccgtgcgctc ggacacgctc aaggatctct ggtcgcagcc
    13561 caatgacctg cgcgaccgca tgctcgcccg cattgacggc cagaatgtct ccgccatccg
    13621 gatcgagtcc ctccttttcc ccgaggtcgg actggagaaa aaggacggct cctggtactt
    13681 gagccgccat caaaagtggg agcccgccaa cggcgagcgc gtgctgcgct tcttcgaggc
    13741 gctcaacacc catgacatcc tcgaattcac cgccgactcc gccgccaacc tcgtccccta
    13801 cggcctcgac aagcccttcc tgaaagtgcg ctggaccgag tccggcgcgg agcacagcct
    13861 gctctttggc aaggacgcgg agggcgccgc ctttttcgcc aagcacggtg acgagcccag
    13921 catctaccgc atcgaggcct ccctcctccc ctccatcccg caggacagca tcaaatggaa
    13981 gggcctcggc gccctgcgct tctcccagtt cgccctgcgc cgcatcaccc tcgccgccgg
    14041 ctccgccccg cccaccgtgc tcgactatga tccctccacc gcccagtgga ccggccagcg
    14101 cgccgggcgt gacatcaccg gcatgataga ccgcgtcaaa gccgacaccc tcgccggaaa
    14161 gctcgccaaa ttcaccgtcc aggactggtc ggcggacatc accgccgcca tccacgccct
    14221 gcgccagccc gccgtccagg tccgcgtcac cctcggcgag cccggcgtca gcaccggccc
    14281 cacccgcgag accctgctga aattcgcccc cacccaggag ggcatggaca ccgccctctg
    14341 gttcggccag gtggacaacg gccccgacgt cttctacatc acccgcaccg ccctcatgga
    14401 cctcctggcc cccgtcttca aaccggagtg aggcgccagc gctggacgcg gcagtgaaaa
    14461 cgcccggctt gagcgccgga gaaaaacctt cggggtggtt tcttggtgcc tccgggcatc
    14521 ctcgccgcag ccttcgggtg gttcatcggt gcctccaggt gccctcgccg cagccttcgg
    14581 ggtggttcac cgggcctccg ggcaccctcg ccgcagcctt cggggtggtt catcggtgcc
    14641 tccgggcacc ctcgccgcag ccttcggggt ggttcaccgg gcctccgggc accctcgccg
    14701 cag
//
LOCUS       DAOMYJ010000125        14672 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Prosthecobacter sp. isolate AMR_MDS_5629
            NODE_1397_length_14672_cov_19.245878, whole genome shotgun
            sequence.
ACCESSION   DAOMYJ010000125 DAOMYJ010000000
VERSION     DAOMYJ010000125.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867850
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prosthecobacter sp. (wastewater metagenome)
  ORGANISM  Prosthecobacter sp.
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Verrucomicrobiaceae; Prosthecobacter.
REFERENCE   1  (bases 1 to 14672)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14672)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.30
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:34:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,742
            CDSs (total)                      :: 4,684
            Genes (coding)                    :: 4,676
            CDSs (with protein)               :: 4,676
            Genes (RNA)                       :: 58
            tRNAs                             :: 55
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14672
                     /organism="Prosthecobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1397_length_14672_cov_19.245878"
                     /isolate="AMR_MDS_5629"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1965333"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            115..1599
                     /locus_tag="PLP58_13405"
     CDS             115..1599
                     /locus_tag="PLP58_13405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ09568.1"
                     /translation="MELPKDIQVVIEPRDSAVEALAAHIRASGHAFSMFDAARLVLAD
                     GERFQARFICAPERAQGLFQTPANGGLYLTREEALSHVLRGAGIEQYYRCEEIELEEP
                     KGDFKAVGVCGISGELLGPPNHHSFQTALLRLHRERFSNLPLEDYKRRVRTETGEEVV
                     AKWKEQQRKGQRWIYIKEQSGDAEPVAFAGRAEMEAHFRRTHGDDACAEVREAVFTGQ
                     PDKTRISHALVLLARNAVDAARKHLFEMSQKLAGGFERRHLKLFKRRAGKLFVCRVKP
                     RPVDPGVVFSERVSRIVEILKSTQGIAINDLVEKLAPAKSSDVSIPGAAPENTETAQD
                     EPVSAENAASPAVRHQLTDEQISVLKDIRWLTNEGYVIEYSDGMVFLGVQGEPASAKP
                     KTDKPAPEPKAVPVAEEQPVSAADVLAESAEAVTHDAASVTDDAAAGEPAPEATAPVE
                     EALEEVVTAEVPVEDTGSSSATETVEEETTASSEEAPASVEAKD"
     gene            complement(1664..4204)
                     /locus_tag="PLP58_13410"
     CDS             complement(1664..4204)
                     /locus_tag="PLP58_13410"
                     /inference="COORDINATES: protein motif:HMM:NF024973.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BatD family protein"
                     /protein_id="HRJ09569.1"
                     /translation="MTGRLRFFLLLLFAAAASGARAQQVRAYVEPAIVKPNQITTFII
                     AVQDGQINSFNNQLRLPLQIQQTSVASTSQQFSIVNGRQSSSIRLSWGVMPVEPGEFV
                     IPSQDLVVNGQTVSTNEVKLIVEQGGPPPGAGGAHDELEPILQIEPGRKEIYQGEVMQ
                     LNCSLYMPRQTQLRRLGLVEIEKSDFAIARFPQQSDQTMTSINGVGYYVLTFRSTLSS
                     LRTGDLKIGPASMEVLVEVPVERGARQNIFPPGFPQPFFNVQTEPRKLTVKSQTVTLK
                     VLPLPEEGRPANFSGAVGDFTLSATATPTTLTVGDPVAVDLVISGSGNFDALTEPQMQ
                     SNGGWKPYPAKRYSIEGNLDQNQTPTLERKIGFSQVFIPEAVHNEVPPFEISFFNSEK
                     RQYITLRTEPIPLAMTPAPVTAATESAQGASAIAPLPIPAADPQPDITDIVIQPRPQA
                     RWLAPTAVLWLQSRAFWTAQAAPVGMLAFAVVLAWVRRRKEALQAGRAGEIRKAWRSL
                     ESARQLPDSGFLHQAAQFIQVAHGLHDVRDPELKKILDRWQNGNFAATPAPPLTSSER
                     AGMFESLVRLLRASLAKTAVLLFLILSMRAAAAVEAAGEESPDSVYQAAIAELEQGNF
                     SKAQYLAESLTKKDPPVLGADLFELIGHARYRQEDPGRAALWYQRARLIDARSPELRQ
                     NLRHLHEQHRFLTFGESSTLSEWSLWLTLNEWLVLASVGLWLVMLPLAWRVLSGRRTQ
                     TWPVTVSVLGLILLVPAGAFAALRPQSALRVADISIITPPDVRAYTAATTTAGTVMDL
                     PPGSQVRVLEKRGAWTYVEIPSRPENLRGWVENRVLIPYWPWDSSLIP"
     gene            complement(4210..6150)
                     /locus_tag="PLP58_13415"
     CDS             complement(4210..6150)
                     /locus_tag="PLP58_13415"
                     /inference="COORDINATES: protein motif:HMM:NF012321.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HRJ09570.1"
                     /translation="MNLTFEDPKLLWWLLTLPALALLRLWTGWKAGRVVGRMTAPRLR
                     EDLVVGVSRWRAGLIFTLQILALGCFILALARPRWGEDRQVQIESGRNVILAMDTSRS
                     MLANDITPDRLTRAKLAAQDLMGALKTDRVGLIAFSGHAYLQAPLTTDHEAILEAIDS
                     LDFTSVPRGGSEIGRALKLAIETFDKNPARNHGLILFSDGGEPDAEIRGYAEQAAKKN
                     ILVLTVGVGTESGALIPDPDPDRQGEFVRDRQGNVVRTRLEEASLVEIARLTRGRYLK
                     LGSQPLAASVVRELLSALQAQANEAREIVKPIERFYWPLSIGVALLMIAWFIRPSPPR
                     RLPAIPALAALAFLISPPAQAADSTFWAALFGKKDDPVAKARAAFEKGDHENAAKLLD
                     RSLREKLPENLRPGYAQALGYSAHQLKDYDRAVAGFSTALENTEPKVQERAHQGLAHS
                     LYDQGDRALAKQPKFTLRAWRDSVRHFDAALEKNPEDEAMRENREFVKKRLDELQQQI
                     AQQEQQGQKGQKGDKQKGEKGEKGEEGEEGDEEDGESGQDGEKDAKGKDRSRKESLGK
                     KDGEGEDEELPEGQIQAANKGEEGDEGEEQQQAEAGEEGEDEATGFSRNEARAFLRTY
                     ADDQKKAQIVRPRDPAVNGKDW"
     gene            complement(6155..7180)
                     /locus_tag="PLP58_13420"
     CDS             complement(6155..7180)
                     /locus_tag="PLP58_13420"
                     /inference="COORDINATES: protein motif:HMM:NF012321.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HRJ09571.1"
                     /translation="MNIPFIHDHIWASPLWLWALALLPVLAWWRGRIGRAPAVVFPTA
                     FLLREMGFKAKARHGGLTFGLVLLSLASAIIALARPQKVISHDEEKSEGIAICLTVDV
                     SLSMLIEDFYIDGRPVNRLIAAKRVMRDFIRGRTSDRVGIVAFAGAPYYPCPMTLDRE
                     WLEANLERVQTGIMEDGTAIGSGIAAAARRLDRENNPSKVIILLTDGANNSGKLSPQD
                     AAKLSATLGQKIYTIAIGTPGVHMIPLPNGRVITSGRQEFDEGTLMEVARIGSGQFYM
                     AQDLNALQDIFDTIDQLEKTEIKKRSIIETQERFFYPLLAAAALLALALLLRLTLLNA
                     APVAVAT"
     gene            complement(7177..7707)
                     /locus_tag="PLP58_13425"
     CDS             complement(7177..7707)
                     /locus_tag="PLP58_13425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ09572.1"
                     /translation="MWIFLAQQAQPKGPPLQPLPHPDLPPVELPPPGYPAWLYIAGAL
                     LVLVMLALIIWLLMRPARGIPVEPKRPFHHALSALREILARAPGQKPGDTSAQVSAVL
                     RTYFWERYHIPAPFRTTKELFQDAAIPATSQRLRRYSALADLWDRLSFAPVPATADEA
                     VKLVEQALAYLEEDRP"
     gene            complement(7707..8618)
                     /locus_tag="PLP58_13430"
     CDS             complement(7707..8618)
                     /locus_tag="PLP58_13430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009962241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HRJ09573.1"
                     /translation="MPIITETDDEKLARILKRVRRIELITRGMVKETLGGQYHSRFKG
                     QGIEFDDFREYQAGDDVRFLDWNVTARMNEPFVRKYIEERELTVMLVVDVSGSGDYGS
                     QEDSKRERAAEVAAVFAFSAVQNQDKVGLILVSDRVEHYLPARKGSAHALRILRDILT
                     IHPRSRRTNLTPALDTALKRIAHRALVVMVSDFHMPDTAWEASLRSLAAKHDVVAAEI
                     RDPQEWQLPDAGRVCLQDPESGEQFILNTSDPAVRRRYAEVISGRQEELTRLLRKNGV
                     ERINVRLDEDYAPAMKAYFRARRRRKR"
     gene            complement(8619..9329)
                     /locus_tag="PLP58_13435"
     CDS             complement(8619..9329)
                     /locus_tag="PLP58_13435"
                     /inference="COORDINATES: protein motif:HMM:NF018154.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zeta toxin family protein"
                     /protein_id="HRJ09574.1"
                     /translation="MPEPHFWIIAGPNGAGKTTLVRDGALAAALSSCEFINPDALTLE
                     YLKEQGISTWAEAETKPDQLKDTFLRAAADSQKLLEQRIAEGGHVAVESVLSTKKYCV
                     LVERVHKAGGRFMLVYVMLHSPHLSRVRINQRSLEGGHDVPSHKLESRWKASLELLPW
                     FANRADQFWIMDNSEAAAGGAGRLVFTGSRHQIRLHGVPASPTRRVASEILTQHSRQA
                     PKGQWRVDLDDSNQTFHI"
     gene            complement(9322..9576)
                     /locus_tag="PLP58_13440"
     CDS             complement(9322..9576)
                     /locus_tag="PLP58_13440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ09575.1"
                     /translation="MSTPSVQEISDKPNLGPVTLGGHLYPADPASPAFTELLMDCFNR
                     GRNAAAARQAQTEALLHSKQAPPLPEKARKLKAKTVKAHA"
     gene            complement(9627..10628)
                     /locus_tag="PLP58_13445"
     CDS             complement(9627..10628)
                     /locus_tag="PLP58_13445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012420813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HRJ09576.1"
                     /translation="MSESAPPTAVPDQETIARASAWVAPLRSEIARVLVGQTELVDRL
                     LVALLTNMHVLLEGVPGLAKTLAVRTLASALHADFKRIQFTPDLLPADVIGTMVYHPK
                     EGNFTPRLGPIFANLVLADEINRAPAKVQSALLEAMQERQVTIGENTYKLPDPFMVLA
                     TQNPIDQEGTYTLPEAQLDRFLFKVRVVYPTPAEERRVLDAMATSAPKLDVSQVTQAK
                     DIVASRAVVNAIHMDEKIRDYIVSIIQATRTPDAVAPHLKLLIRCGASPRGTINLALA
                     SKALAFLNGRNFVIPQDIKDLAPDILRHRILLSYEAEAEGVTSEEVIKQLLDKLPVP"
     gene            10789..11412
                     /locus_tag="PLP58_13450"
     CDS             10789..11412
                     /locus_tag="PLP58_13450"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HRJ09577.1"
                     /translation="MIRNLILDWSGTLADDLGAVVEATNHVLAHFGRSALTREEFRAV
                     FRLPYTEFYQEMLPHAPLAELQQVYLERFPEDHPVPMIEHAEDFLRHAAESGRRMFVL
                     SSAPLEHVSEQARRNGVLGCFEHLHCGVIDKCGHIHELLGKHGLDASETAFVGDMRHD
                     IHAGKSAGVTTIATATGYESVPVLLTAGPDVLVRNLSFLTRMFRLPS"
     gene            11409..11774
                     /locus_tag="PLP58_13455"
     CDS             11409..11774
                     /locus_tag="PLP58_13455"
                     /EC_number="4.1.2.25"
                     /EC_number="5.1.99.8"
                     /inference="COORDINATES: protein motif:HMM:NF014233.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004150 - dihydroneopterin aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006760 - folic acid-containing compound
                     metabolic process [Evidence IEA];
                     GO_process: GO:0046656 - folic acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin aldolase"
                     /protein_id="HRJ09578.1"
                     /translation="MMNTKPPTDEIHLRGLDLPARVGVPDEERAAWQNLRLDATLRLA
                     RRFEDMGDDIRMTPDYAAVAAFARSVAAERPRRLIETLAAEIAERLLSEFPLTGVTLD
                     LRKRVLHGCDEVFVRLTRP"
     gene            11891..12499
                     /locus_tag="PLP58_13460"
     CDS             11891..12499
                     /locus_tag="PLP58_13460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009962923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HRJ09579.1"
                     /translation="METSSEQTKGGGAAEDRELVERAQAGDTRAFDELVRKYTPKLYG
                     MVYNMTSNREDTADLLQEIFAKAYRSLKRFLGKSSFYTWIYSIAVNMTLNYLKKRGRY
                     AKVSLDDVDSGIQNDPEFIKITTANRDTVREVNIHELQKRLNDAMMKLSEDHRTVVTL
                     YDIQGLQHNEISKILGVSEGTVRSRLFYAHRLLQSYLEDFVK"
     gene            12544..13068
                     /locus_tag="PLP58_13465"
     CDS             12544..13068
                     /locus_tag="PLP58_13465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ09580.1"
                     /translation="MMNEFENIQSLIRLKRHETPPEDFVEDFLARFKERQRAELLRQS
                     ARGLLWERISTFMDDMFSPRWTTAAATAAVAVIAAWGALSLVNGGGGGHAELAIAPLP
                     EVIPLQPVLAVESDLIKAEPAARTLEIQAIQLSFNGEVLAFEAPADRLSELAGLGTAS
                     FPVASEILPVSFGN"
     gene            complement(13075..13998)
                     /locus_tag="PLP58_13470"
     CDS             complement(13075..13998)
                     /locus_tag="PLP58_13470"
                     /inference="COORDINATES: protein motif:HMM:NF012692.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HRJ09581.1"
                     /translation="MTLHSTFPSDRLAIGIDLGGTQVKGALVDRESGEILRTALLPTR
                     DGEFENETPVFAMQVAGIIAELETGGARLPVGLAAPGLAAPHGRCITWMPGRMHGLEG
                     FDWPEFLQRETRVLNDAQAALLGEAWTGAAKGCRDVFMVTLGTGVGGAVLTGGRLLTG
                     ASGRGGHLGHMCVDVNAPKDVFNTPGSLEAMIGNATVRERSGGRFASTLELVQAAQSG
                     DEAAMRAWHDSVRKLAAALVSLVNVFDPEVIILGGGIASGAGTALLEPLERWLDEYEW
                     RPGGLKVPLKMASAGEWAGALGVVRGLRSEE"
     gene            complement(14046..>14672)
                     /locus_tag="PLP58_13475"
     CDS             complement(14046..>14672)
                     /locus_tag="PLP58_13475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009961729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HRJ09582.1"
                     /translation="ERAVALAVEAHGRLDALYHVAGGSGRKMGDGPLHEMTDAGLRHT
                     LALNLESLMLSNRAAVRQFLRQGDGGCILNMGSVLGWSPSPEFFASHAYAAAKAGILG
                     FSKAIASHYARDDIRVNVIAPALVETPMSRRAAGDEEILHFVALKQPLDGGRAGVPED
                     VTGAALFLLSRAARYITGQVLAVDGGWTVSEGREESSKLETRNSKSET"
BASE COUNT         2562 a         4451 c         4900 g         2759 t
ORIGIN      
        1 aggggggaat tccgcccgcg aggcgagcgc cgcgagggcc agggaggagg gcagcgtgac
       61 aggcgcgaag gccagcgcca tcaaggcgag caagggcatc agccacgcga gcggatggag
      121 ctgccaaagg acatccaggt ggtcatcgag ccgcgcgatt cagccgtcga ggcgctggcg
      181 gcgcacatcc gcgccagcgg gcacgccttc agcatgttcg atgccgcgcg tctcgtgctg
      241 gcggatgggg agcgctttca ggcgcgcttc atctgcgcgc ctgagcgcgc gcaggggttg
      301 ttccagactc cggcgaacgg gggcctctac ctcacgcgcg aggaggctct ctcccatgtg
      361 ctgcgcggcg cgggcatcga gcagtattac cgctgcgagg agatcgagtt ggaggagccg
      421 aagggggact tcaaagcggt cggcgtctgc ggcatctccg gggagctgct tggcccgccg
      481 aatcatcaca gcttccagac cgctctcctg cgcctgcacc gcgagcgttt ttccaacctg
      541 cccttggagg actacaagcg gcgcgtacgg accgagacgg gcgaggaggt ggtggcgaag
      601 tggaaggagc agcagcgcaa gggtcagcgc tggatttaca tcaaggaaca gtccggggac
      661 gcggagcccg tggcatttgc cggccgcgcc gagatggagg cgcacttccg ccgcacgcat
      721 ggcgatgacg cctgcgccga ggtgcgcgag gctgtcttca ccggccagcc ggacaagacg
      781 cgcatttccc acgcgctcgt tttgctggcg cgcaacgcgg tggacgcggc gcgcaagcat
      841 ctcttcgaaa tgtcgcaaaa actcgcgggc ggtttcgagc gccggcattt gaaactcttc
      901 aagcgcaggg ccgggaagct ctttgtctgc cgcgtgaagc cgaggccggt ggacccgggt
      961 gtcgtgtttt ccgagcgcgt ctcgcgcatc gtggagattc tcaaatccac ccagggcatc
     1021 gccatcaacg accttgtgga aaaacttgcc cccgcgaaat cctcggatgt gagcatcccc
     1081 ggtgccgccc cggaaaatac ggagactgcg caggacgagc cggtctcagc cgaaaacgcc
     1141 gcgtcccccg cagtcagaca ccaactcacc gacgagcaaa tctccgtgct caaggacatc
     1201 cgctggctga ccaacgaagg ctacgtcatc gaatattccg acggcatggt cttcctcggt
     1261 gtccagggcg agcctgcatc ggcgaagccg aagacggata agcccgcacc cgaacccaag
     1321 gccgtgcctg tggctgagga gcagcctgtg tctgccgcgg acgtgctggc tgagagcgcc
     1381 gaagccgtca cacatgatgc cgcgagtgtc actgacgacg cggctgccgg ggagcctgcg
     1441 ccggaggcga ctgcgccagt tgaggaagcg cttgaagagg tcgtcaccgc agaagttccc
     1501 gtcgaagaca ccggatcatc atccgctacc gagactgtgg aggaggagac gactgccagc
     1561 agcgaggaag ctccggcttc cgtcgaagcc aaggactgag cctgaaaacg cggaccagtc
     1621 agaaggccgc ggatttccct attttacgca gcgcccttcc atctcagggg atgagggagc
     1681 tgtcccaagg ccagtagggg atgagcaccc ggttctcgac ccagccgcga aggttctcgg
     1741 gtcgtgaggg aatctcgacg taggtccagg ctccgcgctt ttcaagcacg cgcacctgac
     1801 tgcccggtgg gaggtccatg acggtgcccg ccgtggtcgt tgccgctgtg taggcgcgca
     1861 catcgggcgg ggtgatgatg gaaatgtccg ccacacgcag ggcgctctgg ggacgcagcg
     1921 cggcgaaggc tccggcggga acaagcagga tgaggccgag cacggaaacg gtgacgggcc
     1981 aagtttgtgt gcggcgtccg ctgagcacgc gccaagccag cggcagcatg acgagccaaa
     2041 gcccgacgct ggccaggaca agccactcgt tgagcgtgag ccagaggctc cattcggaga
     2101 gcgtggagct ttctccgaaa gtcaaaaaac ggtgctgctc gtgcagatga cggaggtttt
     2161 gccgcagttc gggtgagcgc gcgtcaatga ggcgggcgcg ctgataccat agggcggcgc
     2221 gtccagggtc ctcctgccgg tagcgggcgt ggccgatgag ttcgaacaaa tccgctccga
     2281 ggacaggcgg gtcttttttg gtcagacttt cggcgaggta ttgagccttg ctgaaatttc
     2341 cctgctccag ttcggcgatg gcggcctggt aaacagagtc ggggctttct tccccggcgg
     2401 cttcgacggc tgctgccgcg cgcatggaga ggatcaaaaa caacaagacg gcagtctttg
     2461 ccagcgacgc gcgcaggagg cggacgagac tctcaaacat gcccgcgcgc tcagagctgg
     2521 tcaggggcgg ggcgggagtt gcggcgaagt ttccattctg ccagcggtcg aggatttttt
     2581 tcaattcggg atcacgcacg tcatgcagac cgtgggcgac ctggatgaac tgcgccgcct
     2641 ggtgcagaaa cccggagtcg ggcagttggc gcgcggattc cagagagcgc catgctttgc
     2701 gtatctcccc ggcgcgtccg gcttgcaggg cctctttgcg ccggcgaacc catgcgagca
     2761 cgacagcgaa ggcgagcatg ccgacaggag cggcctgcgc agtccagaaa gcgcggcttt
     2821 gcagccacaa gacggcggtg ggagccagcc acctggcctg gggacgcggc tggatgacga
     2881 tgtcggtgat gtcaggctgg ggatcggcgg cggggatggg gagcggggcg atagcactgg
     2941 caccctgggc ggattcggtg gccgcagtga cgggcgcggg cgtcatggcc agcgggatgg
     3001 gttcggtgcg cagggtgatg tattgtcttt tctcggagtt gaagaagctg atttcgaaag
     3061 gcgggacctc gttgtgaacg gcctcgggga tgaagacctg ggagaagccg atcttgcgct
     3121 ccagagtggg tgtctggttt tgatccagat tcccttcgat ggagtagcgc ttggccgggt
     3181 agggtttcca gccgccgttg gattgcatct ggggctcggt gagggcgtcg aagtttccgg
     3241 agccggaaat gacgaggtcc acagccacgg ggtcgcccac ggtgagggtg gtgggtgtgg
     3301 cggtggcgct gagtgtgaag tcgcccacgg cgccgctgaa gttggcgggc cgcccttctt
     3361 cgggcagggg caggactttg agggtgacgg tctggctttt gacggtgagc ttgcgcggct
     3421 cggtctggac gttgaagaag ggctggggga agccgggcgg gaaaatgttc tgacgggcgc
     3481 cgcgctcgac gggcacctcg acgagcactt ccatggaggc ggggccgatt ttcaaatcac
     3541 cggtgcgcag ggacgagagg gtgctgcgga aagtcagcac atagtagccg acaccgttga
     3601 tggaagtcat ggtctggtcg ctctgctggg ggaagcgggc gatggcgaag tcgctctttt
     3661 caatttcgac aaggccgagc ctgcggagct gggtctggcg cggcatatag agggagcagt
     3721 tgagctgcat gacctcgcct tgatagattt ctttgcggcc gggctcgatt tgcaggatcg
     3781 gctccaactc atcatgcgct ccaccagcac ccggaggcgg acctccctgc tcgacgatga
     3841 gcttgacctc gttggtggac actgtctggc cgttgaccac aagatcctgc gatgggatga
     3901 cgaactcgcc gggctcgacg ggcatgacgc cccagctcag gcggatggag gaggactggc
     3961 ggccgttgac gatgctgaac tgctgcgagg tggaggcaac ggaggtctgc tgaatttgca
     4021 gcggcaggcg gagctggttg ttgaaggaat tgatctgccc gtcctgcacc gcgatgataa
     4081 acgtggtgat ctggttcggc ttcacgatgg ccggctccac ataagcgcgc acctgctggg
     4141 cgcgggcgcc ggacgcggcg gcggcaaaga ggagaaggag aaaaaatcga agtctgcctg
     4201 tcatgatggt caccagtctt ttccgttcac cgcaggatca cgcgggcgca cgatctgcgc
     4261 tttcttttgg tcgtcggcat aggtgcggag gaaggctcgg gcctcgttgc ggctgaagcc
     4321 ggtggcctcg tcctcgccct cctcgccagc ctcggcctgt tgttgctcct ccccttcgtc
     4381 gccctcctcg cctttgttgg cggcctggat ctggccttcg ggcagttcct catcctcgcc
     4441 ttcgccgtct tttttgccga ggctttcctt gcggctgcgg tccttgcctt tggcgtcttt
     4501 ttcaccgtcc tggccggact cgccgtcctc ttcgtcgcct tcctctcctt cttccccctt
     4561 ctcgcctttt tcacctttct gcttgtcgcc cttttgtcct ttctggcctt gctgctcctg
     4621 ctgggcgatc tgctgctgaa gctcgtcgag gcgttttttc acgaactcgc ggttctcgcg
     4681 catggcttcg tcctccgggt ttttttccag ggcggcgtcg aagtggcgga cggagtcgcg
     4741 ccaggcgcgc agggtgaact tgggctgctt ggcgagggcg cggtctccct ggtcataaag
     4801 gctgtgcgcc aggccctggt gggcgcgctc ctggaccttg ggttcggtgt tttccaaggc
     4861 ggtgctgaag ccggccacgg cgcggtcgta atccttgagc tggtgcgccg agtaaccgag
     4921 ggcctgcgcg tagccggggc ggagattttc ggggagcttc tcgcgcaggc tgcggtcgag
     4981 gagtttggcg gcgttttcgt ggtcgccctt ttcaaaggcg gcgcgcgcct tggcgacggg
     5041 gtcgtctttt ttcccaaaca gcgcggccca gaaggtgctg tccgccgcct gcgcgggcgg
     5101 gctgatgaga aaggccagcg ctgccagagc cgggatggcg ggcaggcggc gcgggggtga
     5161 ggggcggatg aaccaggcga tcataagaag ggcaactcct atagacagag gccagtagaa
     5221 acgttcgatg ggcttcacaa tttcccgcgc ttcattggcc tgggcctgga gggcggaaag
     5281 cagctcgcgg accaccgagg cggcgagagg ctgggagccg agtttcaaat acctgccgcg
     5341 ggtgaggcgt gcgatttcca ccagcgacgc ctcctccagc cgtgtgcgca cgacattgcc
     5401 ctgacggtcg cgcacaaact cgccctggcg gtcgggatcg ggatcgggaa tgagggcgcc
     5461 gctttcggtg ccgacgccga cggtgagcac gaggatgttt ttcttcgcgg cctgctcggc
     5521 gtagccgcgg atttcggcgt cgggctcgcc gccgtcgctg aagaggatga gaccgtggtt
     5581 gcgggcgggg tttttatcga aggtctctat cgccagcttc agcgcgcggc cgatctcgct
     5641 gccgccgcgc gggaccgagg tgaagtcgag cgagtcaatg gcctccagga tggcctcgtg
     5701 atcggtggtg agcggggcct ggaggtaggc atgaccggaa aaggcgatga gaccgacgcg
     5761 gtcggtttta agcgcgccca taaggtcctg cgcggcgagc ttggcgcggg tgaggcggtc
     5821 tggggtgatg tcgttcgcca gcatggagcg cgaggtgtcc atggccagga tgacattgcg
     5881 cccggactcg atctgcacct gccggtcctc cccccagcgc ggccgcgcga gggcgaggat
     5941 gaagcagcct agggcgagga tttggagggt gaaaatgagg cccgcccgcc agcgcgagac
     6001 accgacgaca agatcctcgc gcaggcgcgg cgcggtcatc cggcccacta cccggcccgc
     6061 cttccagccg gtccagagac gcagcagcgc cagggcgggc agggtgagca gccaccagag
     6121 cagtttgggg tcttcaaagg tgaggttcat cgtgtcaggt ggccacggcg accggggcgg
     6181 cattcaacag ggtgaggcgc agcagcagag ccagggcgag cagcgcggcg gccgcgagca
     6241 ggggataaaa gaaacgctcc tgggtctcga tgatgctgcg ctttttgatc tcggtctttt
     6301 cgagctggtc aatggtgtcg aagatgtcct ggagggcgtt gaggtcctgg gccatgtaaa
     6361 actggccgct gccgatgcgc gccacctcca tgagcgtgcc ttcgtcgaac tcctgcctgc
     6421 cgctggtgat gacgcgcccg ttgggcagag ggatcatgtg aacgccgggc gtgccgatgg
     6481 cgatggtgta gattttctgg ccgagcgtgg ccgagagctt ggcggcgtcc tgcgggctga
     6541 gtttgccgct gttgttggcg ccgtcggtga gcaggatgat gaccttgctg gggttgttct
     6601 cgcggtccag gcggcgcgcg gcggcggcga tgccggatcc gatggcggtg ccgtcctcca
     6661 tgatgccggt ctgcacgcgc tccaggttcg cctccagcca ctcgcggtcg agtgtcatgg
     6721 ggcaggggta atacggcgcg ccggcaaagg cgacgatgcc gacacggtcg ctcgtgcggc
     6781 cgcggatgaa gtcgcgcatc acacgcttgg cggcgatgag gcggttcacc gggcggccgt
     6841 caatgtagaa gtcctcaatg agcatggaga gcgagacatc caccgtgagg cagatggcga
     6901 tgccctcgga tttctcctca tcatgcgaga tcaccttttg cggccgggcc agcgcgatga
     6961 tggccgaggc caggctcagc agcaccaggc cgaaggtgag tccaccgtgc cgcgccttgg
     7021 ccttgaagcc catctcgcga agcaggaagg ctgtggggaa aaccaccgca ggcgcgcgcc
     7081 cgatgcgtcc gcgccaccag gccaggacgg gcagcaaagc cagcgcccag agccagagcg
     7141 ggcttgccca gatgtggtca tggatgaatg ggatgttcat ggccggtcct cctccagata
     7201 cgccagggct tgctcgacga gtttcaccgc ctcatccgca gtggcgggca ccggggcgaa
     7261 ggagagcctg tcccacaaat ccgccagcgc cgagtagcgg cgcaggcgct gggaggtggc
     7321 ggggatggcg gcatcctgga aaagctcctt cgtcgtgcgg aagggtgccg ggatgtgata
     7381 gcgctcccaa aaatacgtcc gcagcacggc ggagacctgg gcggaggtgt cccccggttt
     7441 ttgacccgga gcgcgggcca gaatctcgcg cagggcgctc aaggcatgat gaaatggccg
     7501 cttcggctcg acaggaatac cgcgcgccgg gcgcatgagc agccagatga tgagagccag
     7561 catcaccagc acaagcagcg cgccagcgat gtaaagccaa gcgggataac ccggcggcgg
     7621 cagttccacc ggcggtaggt ccgggtgggg caggggttgc aggggcgggc ctttgggctg
     7681 cgcttgctgg gccaggaaaa tccacatcac ctttttctcc tcctgcgtgc ccggaagtaa
     7741 gccttcatgg ccggggcgta atcctcatca aggcgcacgt tgatgcgctc gacgccgttt
     7801 ttgcgcagca ggcgggtcag ttcctcctgc ctgccgctga tgacctccgc gtagcggcgg
     7861 cgcacggcgg ggtcgctggt gttgaggatg aactgctcgc cgctctccgg gtcttgcagg
     7921 cacacgcgtc cggcgtcggg gagctgccac tcctgcgggt cgcggatttc agcggccacc
     7981 acgtcatgct tcgcggccag ggagcgcagg ctggcctccc aggcggtgtc gggcatgtgg
     8041 aaatccgaca ccatgaccac cagcgcgcgg tgggcgatgc gcttgagcgc cgtgtccagc
     8101 gcgggcgtga ggtttgtgcg ccggctgcgc gggtggatgg tcagaatatc gcgcaggatg
     8161 cgcagggcgt gcgcgctgcc tttgcgcgcg ggcaggtagt gctccacgcg gtcgctgacg
     8221 aggatgaggc ccaccttgtc ctggttctgc accgcgctga aggcaaagac ggcggccacc
     8281 tcggcggcgc gctcgcgctt gctgtcctcc tggctgccgt agtcgcccga gccgctgaca
     8341 tccaccacga gcatgactgt cagctcgcgt tcctcgatgt atttgcgcac gaagggctcg
     8401 ttcatgcgcg cggtcacatt ccagtcgagg aagcgcacgt catcaccggc ctgatactcg
     8461 cggaagtcgt cgaactcgat gccctggccc ttgaagcgcg agtggtattg cccgcccagc
     8521 gtctccttca ccatgccgcg cgtgatcagc tcaatgcgcc gcaccctttt gaggatgcgc
     8581 gcgagctttt cgtcgtcggt ttcggtgatg atgggcattt agatatgaaa tgtctggttt
     8641 gagtcatcaa gatcaaccct ccattggcct ttcggagcct gcctggaatg ttgtgtcagg
     8701 atttccgatg ccactcggcg tgtgggagac gccgggacac cgtgaagccg tatttgatga
     8761 cgggaaccgg tgaaaaccag ccggccggca ccgcccgcag ccgcctctga attgtccatg
     8821 atccaaaatt gatccgcgcg gttggcaaac caaggcagca actccaacga agccttccat
     8881 cgcgattcga gcttgtggct aggcacatcg tgccctcctt cgagacttct ctgattgatg
     8941 cgaactcggg aaagatgagg cgaatgcagc atcacataca caagcatgaa gcggccaccc
     9001 gctttgtgga cacgctccac caatacgcag tattttttcg tgcttagcac ggactcgacg
     9061 gccacatgcc ccccttcggc tatacgctgt tcgagcaatt tctgagaatc ggcggcggca
     9121 cgtagaaaag tgtctttcaa ctgatcgggc ttggtttccg cttcggccca ggtggaaatg
     9181 ccctgttcct tcaagtactc caaggtcaat gcgtcaggat tgatgaactc gcaggaagac
     9241 agtgcggccg ccagcgcgcc atcacgcacc agtgtggtct tacccgcgcc attcggaccg
     9301 gcgatgatcc aaaaatgcgg ttcaggcatg agctttcaca gtcttggcct tgagtttccg
     9361 cgctttttca ggcagaggag gggcctgctt ggagtgaagc agggcctctg tttgcgcctg
     9421 tctcgccgct gccgcgtttc tgccgcgatt gaagcagtcc atcagcaatt ctgtgaatgc
     9481 gggtgacgca gggtcggcgg gatagagatg accgcctagg gtcaccgggc cgaggtttgg
     9541 cttgtcggaa atttcctgga cggatggcgt gctcatgatc gtcggttttg ctgggaattt
     9601 agttcttgct gctcgtgcgg caagcctcaa ggcaccggca gcttgtcgag cagttgcttg
     9661 atgacctcct cgctcgtcac gccttcggcc tcggcctcgt aggagagcag gatgcggtgg
     9721 cggaggatgt ccggggcgag gtctttgatg tcctggggaa tcacgaagtt gcggccgttg
     9781 aggaaggcga gcgctttgga ggcgagggcg agattgatgg tgccgcgcgg ggaggcgccg
     9841 cagcggatga gcagcttcaa atgcggggcc acggcgtcgg gcgtgcgcgt ggcctggatg
     9901 atgctgacga tgtagtcgcg gattttttca tccatgtgga tggcgttcac cacggcgcgg
     9961 ctggccacga tgtctttggc ctgggtgacc tggctgacat caagtttcgg cgcggaggtg
    10021 gccatggcgt cgagcacccg gcgttcctcg gcgggggtgg gatagacgac gcggactttg
    10081 aagagaaagc ggtcgagctg cgcctcgggc agggtgtagg tgccttcttg atcaatcggg
    10141 ttttgcgtgg ccagcaccat gaaggggtcc ggcagcttgt acgtgttttc gccgatggtc
    10201 acctgcctct cctgcatggc ttcgagcagg gcgctctgca ctttggcggg ggcgcggttg
    10261 atttcgtcgg cgagcaccag attggcgaaa atggggccga ggcgcggggt gaaatttccc
    10321 tccttgggat gatacaccat ggtgccgatg acatcggcgg gcagcaggtc gggtgtgaac
    10381 tggatgcgtt tgaagtcggc gtgcaaagcg ctggcgagcg tgcgcacggc gagcgttttg
    10441 gcaaggccgg gcacgccttc gagcagcaca tgcatgttgg tcagcagcgc caccagcaag
    10501 cggtccacca gttcggtctg gcccaccagc acccgggcga tttccgacct cagcggggct
    10561 acccaggctg aggctcttgc gatggtttcc tggtcgggga cggctgtggg aggtgcggat
    10621 tcagacatgt ggtgaggaaa aactcaacgg ggggtgggca agagtcaagg aaggagactc
    10681 ccgcaacatg aacgccacga cgcccttgtt ttgccgcaaa aactctgttc cctcgccatg
    10741 cggtcaaaaa ttgccacaag ggcgggtccg cgcacatata gagaatccat gatccgcaat
    10801 ctcatcctcg actggtcggg caccctggcc gacgacctgg gggctgtcgt cgaggccacc
    10861 aatcatgtgc tcgctcattt cgggaggtcc gccctgacgc gggaggagtt ccgtgcggtg
    10921 ttccgcctgc cctacacgga gttttatcag gaaatgctgc cgcacgcgcc cctggccgag
    10981 ctgcagcagg tgtatctgga gcgttttccc gaagaccatc ccgtgccgat gatcgagcac
    11041 gcggaggatt tcctgcggca cgcggcggag tccggccggc gcatgttcgt gctcagcagc
    11101 gcgcccctgg agcatgtcag cgaacaggcc cggagaaacg gagttcttgg ctgcttcgag
    11161 catctgcact gcggtgtcat tgacaagtgc ggccacattc atgaactgct cggaaagcac
    11221 gggctggacg cctccgagac cgctttcgtc ggcgacatgc gccatgacat ccacgcggga
    11281 aagtcagccg gcgtcaccac catcgccacg gccacgggtt acgagtccgt tcctgttctg
    11341 ctgaccgccg gtccggatgt cctggtgcgg aatctttcct ttctcacccg catgttccgc
    11401 ctgccgtcat gatgaatacg aagccgccta ccgatgaaat ccatctgcgc ggactggacc
    11461 tgccagcgcg cgtcggcgtg ccggatgagg agcgtgccgc atggcagaac ctgcggctgg
    11521 atgccaccct gcgtctcgca aggcgcttcg aggacatggg ggacgacatc agaatgacgc
    11581 cggattacgc cgctgtggcc gcttttgcgc gcagtgtcgc ggcggagcgc ccgcgcaggc
    11641 tcattgagac tcttgccgct gagatcgcgg agcgtttgct ttcggagttt cctctgaccg
    11701 gcgtcactct cgatctgcgc aagcgggtgc ttcacggatg cgacgaggtt tttgtgaggt
    11761 tgacccgacc gtgacatgcg gcgtatcatc cgataattcc gaaaaccaag caatccttgt
    11821 actcccgcaa actccgatgg atggtcttct cccagaaaca tgcgcccctg tgagggcgga
    11881 cagcggcatt atggagacct catccgaaca aaccaagggc ggcggcgcgg cggaggatcg
    11941 agaactggtg gagcgcgccc aggccggtga cacccgggcc tttgacgagc ttgtgcggaa
    12001 gtacactccg aagctctacg gcatggttta caacatgacc tccaaccgcg aggacacagc
    12061 ggacctgctc caggagatct ttgccaaggc ttaccgctcc ttgaagcgct ttcttggcaa
    12121 gtcctccttc tacacctgga tctattcgat cgcggtgaac atgactctca actaccttaa
    12181 aaagcggggg cgttacgcca aggtcagcct ggacgacgtg gactccggca ttcaaaacga
    12241 cccggaattt atcaagatca ccaccgcgaa cagggacacc gtccgggagg tgaacatcca
    12301 cgaacttcaa aaaagattga acgatgccat gatgaagctg tcggaggacc accggaccgt
    12361 ggtcacgctc tatgacatcc agggtcttca acacaacgag atcagcaaga ttctcggtgt
    12421 gtccgaaggc actgtgaggt cgcggctttt ctacgcccac cgccttctgc aaagttatct
    12481 cgaagatttt gtgaaatgaa cattctctga cttcttccca atccacccgc tcccacactt
    12541 gccatgatga acgaattcga gaacatccag agcctgatcc gcctcaaacg ccacgagacg
    12601 ccgcccgaag atttcgtcga ggacttcctc gcccgcttca aggagaggca aagggcggaa
    12661 ctcctccgtc aatcggcgcg ggggctgctc tgggagcgta tcagcacttt catggacgac
    12721 atgttttccc cgcgctggac aaccgccgcc gcgacagccg ctgtggcagt cattgcggca
    12781 tggggcgctc tgagtctggt taatggcggt ggtgggggac atgcggagtt ggcgattgcc
    12841 cctctgccgg aagtcattcc gctccagcct gtgctcgctg tggagagtga tttgatcaag
    12901 gcggagccgg cagcgcgcac tcttgagatt caggccattc aactcagctt caacggcgag
    12961 gtcctggcct tcgaagcacc cgccgaccgt ctgagcgaac tcgccggcct tgggactgcc
    13021 tctttccctg tggcctcgga gattcttcca gttagtttcg gaaactgagg ccgctcattc
    13081 ctccgacctt aatcccctca ccacaccgag agcgccggcc cattcgccgg cgctcgccat
    13141 ttttagcggc accttcaggc cgcctggacg ccattcatac tcgtccagcc agcgctcgag
    13201 cggctccagc aaagccgttc ccgcgccgga tgcgatgccg ccgcccagga tgatgacttc
    13261 cggatcgaac acattgacca gcgagaccag tgctgcggcg agttttctga ccgagtcatg
    13321 ccatgcgcgc atcgccgctt catcgccgga ttgcgcggct tgcacgagtt ccagcgtgct
    13381 cgcaaatcgt cccccgctgc gctcccgcac ggtggcattg ccaatcatgg cctccaggct
    13441 gccgggggtg ttgaagacat ccttcggcgc gttcacatcc acgcacatgt ggcccaggtg
    13501 gccgccgcgc ccgctcgcgc ccgtgagcag ccgcccgcct gtgagcaccg cgccgcccac
    13561 gcccgtgccc agcgtgacca tgaagacatc ccggcagccc ttcgccgcgc cggtccatgc
    13621 ctcgccaagc agggcggctt gcgcgtcatt gagcacccgc gtctcacgct gtaaaaactc
    13681 cggccagtca aagccctcca gcccgtgcat ccgccccggc atccaggtga tgcagcgccc
    13741 gtgcggcgcg gccagccccg gcgcggccag gcccacgggc agacgcgcgc ctcctgtctc
    13801 caactccgcg atgatcccgg ccacctgcat ggcaaagacg ggcgtctcgt tttcaaactc
    13861 cccgtcccgt gtcggcagca gcgccgtgcg caggatctcg ccgctctccc ggtccaccag
    13921 cgcgcccttc acctgcgtgc cgcccaggtc aatgccaatg gccagccggt cggaggggaa
    13981 tgtggagtgc aatgtcatgg ggcggggtgt tgaagactac cgttcgaaat tcgaagcccc
    14041 gtgcttcatg tttcggactt cgagtttcga gtttcgagtt tcgaactttc ctcccgcccc
    14101 tccgacaccg tccagccccc gtccaccgcc agcacctggc ccgtgatgta gcgcgccgcg
    14161 cgtgacagca aaaacagcgc agcccccgtc acatcctcag gcacgccagc gcgcccgcca
    14221 tccagcggct gcttcagcgc cacaaagtgc aggatttcct catcccccgc cgcccgcctt
    14281 gacatcggcg tctccaccag cgccggggcg atcacgttca cgcggatgtc atcccgcgcg
    14341 tagtggctgg cgatggcctt gctaaagccc aggatgcccg ccttcgccgc tgcataggca
    14401 tgactcgcga aaaactccgg ggaaggggac caccccagca cactgcccat gttcaagatg
    14461 cagccgccgt ccccctggcg cagaaattgc cgcaccgccg cccggttcga gagcatcagc
    14521 gactccaggt tcagtgcaag cgtgtgccgc aggcccgcgt ccgtcatctc gtgcagcggc
    14581 ccgtcgccca ttttacgacc gctgccgccc gccacatggt agagcgcgtc cagccgcccg
    14641 tgcgcctcca ccgccagcgc caccgcccgc tc
//
LOCUS       DAOMYJ010000126        14521 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Prosthecobacter sp. isolate AMR_MDS_5629
            NODE_1407_length_14521_cov_23.910756, whole genome shotgun
            sequence.
ACCESSION   DAOMYJ010000126 DAOMYJ010000000
VERSION     DAOMYJ010000126.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867850
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prosthecobacter sp. (wastewater metagenome)
  ORGANISM  Prosthecobacter sp.
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Verrucomicrobiaceae; Prosthecobacter.
REFERENCE   1  (bases 1 to 14521)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14521)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.30
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:34:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,742
            CDSs (total)                      :: 4,684
            Genes (coding)                    :: 4,676
            CDSs (with protein)               :: 4,676
            Genes (RNA)                       :: 58
            tRNAs                             :: 55
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14521
                     /organism="Prosthecobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1407_length_14521_cov_23.910756"
                     /isolate="AMR_MDS_5629"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1965333"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1055)
                     /locus_tag="PLP58_13480"
     CDS             complement(<1..1055)
                     /locus_tag="PLP58_13480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ09583.1"
                     /translation="MKNNFLIPLLVIANLVTVAQALAQAPVIDREPYRKLYDINGDLT
                     GVEFPDPDVPEHHPAPQFNFPTTLRKDASSLINAESIIHLAIDASGPGLSYQWKKGNT
                     NLVNMNDGTRVIDGANGPELRVFPVTVGDFGIYRCVVSNSSGTVTSIPVRITVLDVFT
                     PSTNIIAASSSATASLSVTVNSATAVTKFRWWKMVGAAVDPINDTMISDGGAFSGATK
                     NVLKIKTPDGSNAGTYYCVVTAYNTFMYSGPKNLIVVPAPESRLVQLGAPLEFIAMPQ
                     GPAALLAQLTYQWRKDNAPLGSETNSTLSIASAQLADQGSYSVDVTHPDAGTVTSLPG
                     TATVIDSSTAYKVVVQG"
     gene            complement(1094..2446)
                     /locus_tag="PLP58_13485"
     CDS             complement(1094..2446)
                     /locus_tag="PLP58_13485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ09584.1"
                     /translation="MQPTFHQPETFARKLAIVMAVASAVAVSAQANLTKLPHRESVPV
                     GLESKISGASIVSVRGIHNSEALLSGPTAGVHVAGGKSEVVLKFPRQAVLGSLSFVND
                     GMEGEFRCFSSVDNATWIALGVNQIQADDRQVLINPGSCQGKFVKLEFNALKSGTIRC
                     FRILGDETDADYQVTQNINGVGVPVNFADGIGGGRMIYATVGGDGYAEKMIVYDLGQP
                     RQLTEIGSVHSRKTVQLKVFGFEMLPEVENWKGRRVLDVKSVQQGANLIAEMSDAGEG
                     IIQMTLGKPVTARYVALNWRSDKDLSDFISYDVALIGTAVVSHDDKLDTVASLASGAV
                     GEKNQTVAQALGGEASEVNNPKARNNEGLVDEDQSEFVTASAPRSLRQYTEARDIIAG
                     MGFAGAGGGIGGMSFRKAAGLGSGGRAKADEGGEEAAEDEEFVGPPVYLEWSRAPSAP
                     "
     gene            complement(2614..3474)
                     /locus_tag="PLP58_13490"
     CDS             complement(2614..3474)
                     /locus_tag="PLP58_13490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ09585.1"
                     /translation="MPDFSGLIESFQRIPSTTYVTAGGAAMVAFLAGLAVARGVVRQI
                     LGMISLGVSVMAGIYVFQHRAEVFGSIGAGMSTDRLLMLSAGAGLLTYFLCRGIAHLL
                     AGLGVLSLLGGLTGWKGGVLSLIPSGFLLWASALLLRLLGSVYGMENAAEVQKQGEKI
                     SGRLSAWLHHMSQQVDRSSLGSLAVRMDPFDMRARANLSRLLILWPDGRVWQQIAARG
                     PQTAQALNHSEIIALGKDAKVRQAIERQDFAGLMQLPQIREAAANPELEPFLRGLALE
                     QAMDAIVYQR"
     gene            complement(3723..5102)
                     /locus_tag="PLP58_13495"
     CDS             complement(3723..5102)
                     /locus_tag="PLP58_13495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009963496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1501 domain-containing protein"
                     /protein_id="HRJ09586.1"
                     /translation="MTASSDFSRRAALKAAACGFGSVAFSGISTAAALPRGLHHAPRA
                     KRVIFLFMHGGPSQVDTFDYKPRLQAEDGRKLPFAPAKNLDPSATSQAKLLGSPWKFR
                     QHGESGLWGSELFPQVSKRLDDICVIRSMQSKGQSHGQAVCMIHTGADNFVRPSVGAW
                     VSYGLGAENADLPAFVSISPPATHGGPRNYGSAFLPAAHQGVTVGRAGKLAKDAGFRF
                     LRSDSDAGRQQRKMELIQAMNRGHYERVRHAPVEGMIQSMELAFRMQSAAPAVMDIEG
                     EPEHIKTLYGIGDEVTDNYGRQCLLARRFAEAGVRYIQVSTPNVWDQHSNLVSGHEKN
                     AAAVDRPIAGLLVDLKQRGLLEDTLVVWGGEFGRTPIVQGANGRDHNPQGFTMWLAGG
                     GVKGGLAYGETDDYGYYAVRDKVHMHDLHATILHLMGIDHLRLTYRHAGRDFRLTDVY
                     GEVVAGIMA"
     gene            complement(5219..12343)
                     /locus_tag="PLP58_13500"
     CDS             complement(5219..12343)
                     /locus_tag="PLP58_13500"
                     /inference="COORDINATES: protein motif:HMM:NF024777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HRJ09587.1"
                     /translation="MIFFRLAFAFSFLVVSAVAQVTTTGFNNAFRPEAPGTLLSHPVT
                     NGSEPIGRTTSINYLNGWIIVGAEQPGSRDGSDWLMRVYDIADPANPVRRFPSDFGLA
                     YEGDFWYFGNVGVHAHGTMQWDSLLLPQVMRVQGFGGLVERGGTNGIPQLSQVPIGYD
                     RGSMAGPWEVNALWYNSPDANIDIRQAYLTGSGFVAFNTVASLDHVGQFGGGDWHPML
                     FGDLLIMARSGVAGHDGVVVYRLEYHNFDDPVRANRSITPHFVGSLDGGFQGYWPNLF
                     SDGTGLYVIGSTTDILIGADITQAADPAGDGSVHVAASLTVPNFTNASYPVYQDNFGF
                     IHNRKIDMTRFLAGDANPIVLTLNETNPPRPPGAPALPGGAIVGVDTSQMSLPLGNLW
                     LTGGYPIPGTNQGLGVWVHQQEPDTAPPQVSYHIPQTNRVNYPRHAPLSFLLHEHSRA
                     GGPRNGIDFTVRPVGEGDVLGAFVPGFLIHDFAGVLTFTPDAGLAADTTYQVDFLSDP
                     GTETGFRDAAGNYIEPYSFRFATGGGINATAPPVIGSVTADAYQPAPSQQVTVTVAAT
                     GDPVLEYRFNFDGTWSDWDTDNFASHTYTSAGRPRVLVQVRDGHGSIVHGSLRLLVIT
                     PPPAGPRPTQSSTLAVGDDAGTRRVWSVNPDANTVTVVNAVTGTKEDEYAVGADPRGI
                     ARDVNGRYWVTCHDSDEIRVLNADGSAHATIPLAYGSAPHGIAPSPDGTLLYVTMFGS
                     GQVHRYSAAAPGDAPLTVTGMDTPRAVAISADGSRVLVTRFISPELHAEVREFNALLT
                     AVRTFTLSSANTLDGGDRAAGVPNYLAGIAISPDGTRAAIVSKQDNTLRGTFFGVGNL
                     THETTVRAVISFLDLNSNAEIPHSRRDFDNSDSPSAVTYTPLGDMLLVTHQGNNRVVG
                     MDALNLAPLTEQIVQGSMITQPAVIAFEVGTGLAPQGVLMDGTSNRLFTQDFMGRSVT
                     VRNAAPLLLQNQTSLPLVATTPAVDTELLTAGVLLGKQIFYNAADPRMSADSYISCAT
                     CHIDGGHDGRVWDFTGRGEGLRRTTDLRGRSGLGHGAVHWSGNFDEIQDFEHDIRGPF
                     GGTGFLNLTPQQFAGQHPSPASGKTGLSADLDALAAYVSSLTPEHTPRSPHRNANGML
                     TAAAVAGQAVFTAQNCATCHSGTSFTNNALMNVGTQSALSGSRLGAVLPGIDTPSLHG
                     LHATRVYLHHGQAATLGDVFDYAGGSWLQGAAGEFFGGGTAPFNDDPAQGGGGFNRGY
                     FNGSAAFLGGTDGVSGVRFTNVDGGTGGMARIALRYVRQYGGGTAHVSINGAQQTIGV
                     ERQFPDNGWQVSGWRWLVIEAALNAGTTNTIEVLRGNGDLFLNGILVANASQLVIAQP
                     HHVVESLPSGERDNLLAYVRQIDGRDAAGVPLPPPASPSPQPPGIVSEPADTMLAAGN
                     ALHFVVAVSGTGPFDFVWRRGVTVVGTNSPELHIASVNAGDAGSYTVEVTNAHGDVTT
                     TPAQVTVNDALSVVTTSLPVATVGAAYSTNLAAAGGVGTRDWSITGGVLPLGMSLSSG
                     GVLSGTVNAPARAIFTVQVSDSSGSASRELQLDVQPAGGFVNDPDLILHYTFDEGGGT
                     QVWDISPAGNNHATTVGNAHWVPGGRFGGAYGPADASATLNRFFPANQGDLDFVPRGD
                     AFTVSVWVRSTFVGGYRTIIGKDDGQISGYTQMRLWATNPLTNVQGANGGQFGGQLAT
                     TPALNDGQWHLLTMTSFNDAGTWRTRVYYDDGMLFNQFNTGPGGTTPGLLRIGDTTLG
                     GNSWNGQLDDLRIYRRALSPSEVAQLYSPPVGPTVNIALAPGQQADSARPYVEFDATF
                     SEPVTGLTVDDFTHSGTHALLMTLVEGTQYRLRIAGYTAGVVSAQLPAARVTAVSDGE
                     PNNASNIASVTYSPPVDDDLAPLSDEFEDAATLADWQRNYLVEGWTGANKLEVWDIDT
                     SREGHMRLVPYASSWYQPYTGAYAFKNVTGDFVATMRMHAARRGGLPGRPVSTYSLGG
                     IMVRTPRGFTNAAPNPDPGPGVVLPWPPSGYTTPWTPDSENYIFLSYGYADAAMWGNV
                     PDTWYNEVKTTINGVSTLYATQSGIPVNTDLVTLQVVRSGNVFVVLRRHGEGGPWIIE
                     NRFTRNDMPATLQLGITTYTDWPSASAQDAFHHNRTVNTGGNPDLVADADYFRLRRPA
                     TAVSPATLQSLPVTGQGGAPVMLTGTGLETALGDSMNTAHQPGQTFDDWLRDHLTPAQ
                     LILSALTSPAADANGNGIGNAVEFALGTTDSSPLHLEILGPPEARVARVTTTRNALAR
                     GMTLVIESNTSLDPGGWTALATSVDGGEPAGTASINETGDALRTLTVETPTGGQVLFV
                     RARVVMTTSP"
     gene            12524..14095
                     /locus_tag="PLP58_13505"
     CDS             12524..14095
                     /locus_tag="PLP58_13505"
                     /inference="COORDINATES: protein motif:HMM:NF015585.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialate O-acetylesterase"
                     /protein_id="HRJ09588.1"
                     /translation="MRLPSLPLSTLLVSLTWTARAELRVPAIFGDHMVLQQKQANPVW
                     GWDTPGTEVTVQFAGQTKSAKAGPDGKWTVKLDPAPANTRPATLSIQGSSARQFQDVL
                     VGEVWVCSGQSNMGFTLSGTWDAELDKAQARHPRLRLITVPRVGTQEPQDDFKGAWQP
                     CTPENAGAFTAVGYHFGRMLHQMLDVPVGLINNAWGGSACEAWVSRDVLEKDGRFGEM
                     MARWRQTEQSFTQEGFDKQMEAHKAKAAAWLKERQQALKSGARFTKAAPRAPQNPMTG
                     QHRPGNLYAGVLHPTIGYGIKGAIWYQGETNAARAHEYGHLFPLMIQHWRSEWKQGDF
                     PFYWVQLADYLAEKPEPGDSSWAELRESQTKTQNAVKNGGQAVIIDLGEANDIHPKNK
                     RDVAERLARIALARDYGVDIRWRSPEFKSAEFQGGKAVVTLDTFGSSLRTVDVSEVKG
                     FALCGEDRKWVWAGAKLLGKDKVEVSAKGVPNPVAVRYAWSDNPACNLFSVEGLPVTP
                     FRSDDFPMITAPAAQ"
     gene            complement(14161..>14521)
                     /locus_tag="PLP58_13510"
     CDS             complement(14161..>14521)
                     /locus_tag="PLP58_13510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ09589.1"
                     /translation="ALAAGGDAVARGVEGVGRGAAGFDDRLGDEVDAVAVRAGGEDAS
                     LWLGQLKTMIMNGVKLQSWHTPHMRNHALLLMNQAHHHLANLIFDLMASAIALQSALS
                     DSAWNVIQPRRLQEAWS"
BASE COUNT         2841 a         4606 c         4357 g         2717 t
ORIGIN      
        1 ttgccctgaa ccaccacttt gtaggctgtc gagctatcaa tgacagttgc agttcccggc
       61 aaagatgtca ccgttcccgc gtctggatgt gtgacatcca cagaatacga tccttggtcg
      121 gccaattgtg cgctcgctat gctcagtgtt gaatttgttt cagaaccaag gggggcattg
      181 tctttgcgcc actgatatgt caactgcgcc agtagtgctg cgggtccttg aggcatggca
      241 atgaattcaa gcggggcacc gagctgcaca agtctggatt caggcgctgg aacaacgatt
      301 aaattcttgg ggccgctgta catgaaggtg ttgtaggccg tcaccacaca gtaataggtg
      361 ccggcattgc tgccatccgg ggttttaatt ttgagcacat tttttgtcgc cccggaaaat
      421 gcgccgccat ccgaaatcat tgtgtcgttg atcgggtcca cggctgcacc taccattttc
      481 caccatctga atttggtaac cgccgtggcg gaatttacag tcacggagag agatgcggtg
      541 gcggaagatg atgcagcaat gatatttgtg gatggcgtga acacatcaag aaccgtaatt
      601 cgaacgggta tgctggtcac tgtcccagaa ctgttgctaa ccacgcatct gtagattcca
      661 aagtcaccca cagtcacagg aaaaaccctt agttcaggcc cgttggcccc gtctatcaca
      721 cgggtcccgt cgttcatgtt caccaaattg gtattgcctt ttttccattg ataagacaag
      781 cccggacccg atgcatcaat cgccaaatga atgatgcttt cagcattgat cagagaactg
      841 gcgtccttcc gcagtgtggt cggaaaattg aattgcggag cagggtgatg ttcagggaca
      901 tctggatcgg gaaattccac gcccgtcagg tctccattga tgtcgtatag ctttcgatag
      961 ggttcgcgat caatgaccgg agcctgagca agggcctgcg caaccgtcac aagattggct
     1021 attaccagaa gtggaatgag aaagttattt ttcacagcgt tgaagttggt tggggtgatt
     1081 tctgtttagt ttctcatgga gcactggggg cgcgagacca ttccaagtaa acgggcggtc
     1141 ccacaaattc ctcatcttca gcagcttcct cgcctccctc atccgccttg gcacgaccac
     1201 cactcccaag gcccgcagcc ttcctgaatg acatgccgcc aattccacca cccgctcccg
     1261 caaatcccat tccagcaatg atgtcccgag cctctgtgta ttgacgaagc gagcgaggcg
     1321 cgcttgcggt gacaaattca gactggtctt catcaacaag gccctcgttg ttccgcgcct
     1381 ttggattatt gacttcggat gcctctccac ccaaagcctg tgcgactgtt tgattttttt
     1441 ccccaacagc ccctgaagcg agactggcga ctgtgtccaa tttatcatca tgactcacaa
     1501 cggccgtccc gatcagcgcg acatcatagg aaatgaagtc ggacaagtcc ttatctgaac
     1561 gccagtttaa tgcaacatac cgggctgtaa caggtttgcc cagggtcatt tgaatgatgc
     1621 cttcacccgc atctgacatt tccgcgatca gatttgcgcc ttgctgcacg cttttgacat
     1681 ccaggacacg ccttcccttc caattttcca cctcgggcaa catttcaaaa ccaaacactt
     1741 tgagttgcac cgttttgcgt gaatgaacgg agccaatttc cgtcaattga cgaggctggc
     1801 caagatcgta aacgatcatt ttttcggcgt agccgtcccc gcccactgtc gcataaatca
     1861 ttcgcccgcc tccaatgccg tccgcgaaat taaccggcac acccacgcca ttgatgtttt
     1921 gcgtcacttg gtaatccgca tccgtctcgt cacccaggat gcgaaagcag cggatcgtcc
     1981 cgctttttaa tgcattgaat tcaagcttga cgaatttacc ctgacaactg cccgggttga
     2041 tcaggacttg tcggtcatca gcctgaatct gattcacgcc aagggcaatc catgttgcgt
     2101 tgtccacgct tgagaagcat cgaaactccc cctccattcc atcattcacg aaagacaagc
     2161 tgccaaggac cgcctggcgc ggaaacttca gaacgacttc acttttgcct cccgccacat
     2221 gaacccccgc agttgggccg ctgagaagcg cttcgctgtt gtggataccg cgcacggaga
     2281 caatcgaggc accggaaatt tttgattcca gtcccactgg cacagactca cgatgaggca
     2341 gcttggtcaa atttgcctgg gcgctgactg ccacagcgga tgccaccgcc atcacgatgg
     2401 cgagtttgcg ggcaaaagtt tctggttggt gaaacgtggg ctgcatattt tttgatttaa
     2461 ttattaaccg cttaaggaga accaatccgc tgaattgatg caagaaaaaa ttccgattct
     2521 tttataattt attgatttta aatggtttaa gaagcgttgc ttcaccgtct gccgggaagg
     2581 atttcgccgc aatttcacat tctttttcct ctttcatcgt tgatatacga tcgcgtccat
     2641 tgcctgttcc agtgccaatc cccgaaggaa tggctcaagt tcagggtttg ccgcagcctc
     2701 gcggatctgg ggaagctgca tcagtcccgc aaagtcctgc ctttcaatgg cctggcgcac
     2761 cttggcgtct tttccaagcg cgatgatctc ggaatgattg agtgcctgcg ctgtctgtgg
     2821 tccccgggct gcgatttgct gccagacgcg accgtccggc cagagtatca atagacgaga
     2881 gagattggcg cgtgcgcgca tgtcgaaagg gtccatcctc acggccagcg aacctaggct
     2941 gctgcggtcc acctgctggc tcatatggtg cagccaagcg gagagcctgc cgctgatttt
     3001 ttcgccttgt ttctgaactt cggcggcatt ttccattcca tacacactgc cgagcagacg
     3061 caacagcaac gcggatgccc agagcagaaa acctgatgga atcaagctga gaacgccccc
     3121 tttccagccc gtcaatcccc caaggaggct cagcaccccc agccccgcca ggagatgcgc
     3181 aatgcctctg cacaaaaaat atgtcagcag ccccgcaccg gcggacagca ttaaaaggcg
     3241 gtccgtgctc attccagcgc cgatgctgcc gaagacctcc gcccgatgct gaaacacata
     3301 gatgcccgcc atgaccgaaa cccccaggct gatcatgccc agaatttgcc tgaccacacc
     3361 gcgagccacc gccaatccgg ccagaaatgc caccatggca gcaccgcccg ctgtcacata
     3421 ggtcgtggac gggatgcgct ggaagctttc gatgaggccg ctgaagtctg gcatgggcgt
     3481 tgggagcaat gctccacggc catcatcaaa cccccggcgg tcccgacccg caagccgatt
     3541 atcaagccgg gccatgtagc cgaagccgca ccatcgatca ccacgcaagg tttggggcgc
     3601 acagtggtcc aattacaccc ggaccctttt tccttggagg acccgcttct gcgtccgcga
     3661 ttgcgccgtt ccacagcatc ggcctgcaag acggagtcct ccaactatcc gatccgccgg
     3721 actcacgcca tgattccggc caccacctct ccatacacgt cggtgaggcg gaaatcccgg
     3781 cccgcatggc ggtaggtgag ccgcaggtgg tcaatgccca tgagatgcag aatcgtcgcg
     3841 tgcaggtcat gcatgtgaac cttgtcccgg acagcatagt agccgtaatc atccgtctcg
     3901 ccgtatgcca gcccgccttt cacacctcca ccagccagcc acatggtaaa tccctgcggg
     3961 ttgtggtcgc ggccgttggc gccctgcacg atgggcgtgc ggccgaactc gccaccccag
     4021 accacgagcg tgtcctccag gagtccgcgc tgtttcaaat ccaccagcag tccggcgatg
     4081 ggccgatcca cggcggcggc gtttttttca tggcccgaga ccagattcga atgctggtcc
     4141 cagacattcg gcgtcgaaac ctggatgtaa cgcacacccg cctcggcaaa acgccgcgcc
     4201 agcaagcact ggcggccata gttgtccgtg acttcatcgc cgatgccgta aagcgtcttg
     4261 atgtgctccg gctcgccctc aatgtccatg actgcgggcg cggcactttg cattcggaag
     4321 gccagttcca tgctttgaat catgccctcc accggcgcat ggcgaacgcg ctcgtaatgc
     4381 ccccggttca tcgcctggat cagctccatt ttgcgctgct gccttcccgc atcggagtcg
     4441 gatctcaaaa agcggaaacc ggcgtccttc gccagcttgc ccgctcgccc cacggtgacg
     4501 ccttgatgcg ctgcgggcag aaaggcggag ccataattgc gcggcccgcc atgcgtggcc
     4561 ggaggactta tgctgacaaa agcgggcagg tcggcgtttt ccgcgcccag gccgtagctc
     4621 acccaggcac cgaccgaggg acggacaaaa ttatccgcgc ccgtgtggat catgcacacc
     4681 gcctgcccgt ggctttgccc cttgctctgc atggagcgga tcacgcagat gtcatcgagc
     4741 cttttcgaaa cctgcgggaa cagttcgctg ccccacaatc cgctctcccc atgctgccgg
     4801 aacttccacg gcgacccgag cagcttagcc tggcttgttg ctgaaggatc caggttcttg
     4861 gctggcgcga atggcagctt cctgccatct tccgcctgca ggcgcggctt gtaatcgaag
     4921 gtgtcgacct gcgacggtcc gccatgcatg aaaagaaaaa tcacccgttt cgcccttggc
     4981 gcatgatgca gcccgcgcgg cagcgccgcc gctgtgctga tgcctgaaaa cgccaccgag
     5041 ccaaaaccac aggcggcggc cttcagggcg gcacggcggg aaaaatcaga cgaagcagtc
     5101 atgaggatgg acgaatggaa gaccagacac aggcagtgtc atgcaatccg tactacgggc
     5161 aaagctccaa attgcatgtc acatgccgca caccctctcc ggccagccgc cttttttttc
     5221 acggactcgt cgtcatcacc acacgggcac ggacaaaaag gacttgcccc ccggtcggag
     5281 tctccaccgt gagagtgcgc aatgcatccc cggtctcgtt gatgcttgcc gttcccgctg
     5341 gctcacctcc atccacactg gtcgcgagcg cggtccagcc cccggggtcc aggctggtgt
     5401 tggactcaat gaccagggtc atgccccgag cgagcgcgtt gcgagtcgtg gtcacgcgcg
     5461 ccacccgcgc ctcaggcggg ccgaggatct ccaagtgcag ggggctggaa tcagtcgtgc
     5521 caagggcaaa ctccaccgcg ttgccaatgc cgttgccatt ggcatccgcg gcaggcgaag
     5581 tcaaagcgga aaggatgagc tgggcgggag tcaaatgatc acgcagccag tcgtcaaagg
     5641 tctgacccgg ctgatgcgcc gtgttcatgc tgtcgcccag cgcagtttca agaccagtgc
     5701 cggtgagcat gaccggcgcg ccgccctgac cggtgacggg caggctttgc agcgtggcgg
     5761 ggctcacggc ggtggcgggc cggcggaggc ggaaatagtc ggcatcggcc acgaggtcgg
     5821 gattgccgcc tgtgttcacg gtgcggttgt gatggaaggc atcctgcgcg gaggcggagg
     5881 gccagtcggt ataggtggtg atgccgagtt ggagcgtagc gggcatgtca ttgcgcgtga
     5941 agcggttttc gatgatccac ggaccgcctt cgccatgccg gcgcaggacg acaaagacat
     6001 tgccggagcg cacgacctgg agcgtgacga ggtccgtgtt cacggggatg ccgctttgcg
     6061 tggcgtagag cgtggagacg ccgttgatgg tggtcttcac ttcgttgtac caggtgtcgg
     6121 gcacgttgcc ccacatcgcc gcgtcggcgt agccgtaaga gaggaagatg tagttttccg
     6181 agtccggcgt ccacggcgtg gtgtagccgc tgggcggcca gggcagcacg acgccggggc
     6241 cgggatcggg gttcggcgcg gcattggtga atccacgcgg cgtgcgcacc atgatgccac
     6301 cgaggctgta ggtggagacg ggccgtccag gcagaccacc acggcgcgcg gcgtgcatgc
     6361 gcatcgtcgc cacgaagtca ccggtgacat ttttgaaggc atacgcgccg gtgtagggct
     6421 ggtaccaact gctggcatac ggcacgagcc gcatgtggcc ttcgcgcgaa gtgtcaatgt
     6481 cccacacctc cagcttgttc gcaccagtcc agccttcgac gaggtaattg cgctgccagt
     6541 ccgcgagcgt ggccgcgtct tcaaactcat ccgagagcgg cgcgaggtcg tcatccaccg
     6601 gcggcgagta agtgacggag gcgatattgg acgcattgtt cggctcgcca tcgctcacag
     6661 ccgtgacgcg cgcggcggga agctgcgcgg agacgacacc cgccgtgtaa ccggcgatgc
     6721 gcagacgata ctgcgtgcct tccacgagcg tcatgagcag ggcgtgcgtg ccgctgtgcg
     6781 tgaaatcatc cactgtcagg cccgtgactg gttcagaaaa ggtcgcgtca aactccacat
     6841 atggacgggc gctgtccgcc tgctgtcccg gtgccagcgc gatgttcacc gtggggccga
     6901 cgggcgggct gtaaagctgt gccacctcgc ttggtgacaa agcacggcga taaatgcgca
     6961 ggtcgtcgag ctgtccgttc caggagttgc cgccgagcgt ggtgtcgccg atgcgcagca
     7021 ggccgggcgt ggtgccaccg gggccggtgt tgaactggtt gaacagcatg ccgtcatcgt
     7081 aatagacgcg tgtgcgccag gtgcccgcgt cgttgaagct ggtcatcgtc aggagatgcc
     7141 actggccgtc gttcagcgcg ggcgtggtgg cgagctggcc gccaaactgc ccgccattcg
     7201 cgccctggac attcgtcagc gggttcgtcg cccacaggcg catctgcgta tagccgctga
     7261 tctgtccgtc atccttgccg atgatcgtgc gatagccgcc cacgaaggtg ctgcgcaccc
     7321 agacggaaac cgtgaaggca tcgccgcgcg gaacaaagtc caagtcacct tgattcgcgg
     7381 ggaagaagcg gttcaacgtc gcgctcgcat ccgccggacc ataagcgcct ccgaaacgcc
     7441 cgcccggaac ccagtgcgcg ttgcccaccg tggtcgcgtg gttgttgcca gcgggtgaga
     7501 tgtcccacac ctgcgtgccg ccgccttcgt caaaggtgta gtgcaggatg agatcgggat
     7561 cgttcacaaa gccgcccgca ggctgcacat cgagctgcaa ttcacgcgag gcgctgccag
     7621 agctgtcact gacttgcacg gtgaaaatcg cgcgtgccgg agcattcacg gtgccactaa
     7681 gcacgccgcc gctgctcagg ctcatgccga gaggcaaaac accgcctgta atgctccagt
     7741 cgcgtgtgcc aaccccgcct gcggcggcca gattcgtgct gtaagccgcg cccacggtcg
     7801 ccaccggcag cgaggtcgtg accacgctca gcgcgtcatt caccgtgacc tgcgcggggg
     7861 tcgtggtcac atcgccatgc gcattggtga cctccaccgt gtagctgcca gcatcgccag
     7921 cgttcacact ggcgatgtgc agctccgggc tgttggtgcc gaccaccgtc actccgcgac
     7981 gccacacaaa atcaaacggt cccgtgccgc tcacggcgac gacaaaatgc agcgcgttgc
     8041 ccgcggccag catggtgtcc gcaggctcgc tcacgatgcc cggcggctgt ggcgatggcg
     8101 aagcgggcgg cggcaaaggc acgccagcgg cgtcgcgccc gtcgatctgc cgcacatagg
     8161 ccagcaggtt gtcgcgttca cccgacggca ggctttccac cacatgatgc ggctgcgcga
     8221 tgacgagctg cgaggcgttc gcgacgagga tgccgttcaa gaagaggtcg ccattgccgc
     8281 gcagcacttc aatcgtgttt gtggttcccg cgttcagcgc cgcctcgatc accagccagc
     8341 gccaaccgct gacctgccag ccattgtccg ggaactgccg ctccacgccg atggtctgct
     8401 gcgcgccgtt gatgctcaca tgcgcggtgc ccccgccgta ttgccgcaca taacgcagcg
     8461 caatgcgcgc catgccaccc gtgccgccgt ccacattggt gaaacgcacg ccagacacgc
     8521 cgtccgtgcc gccgaggaag gcagcggagc cgttgaagta gccgcggttg aacccgccgc
     8581 cgccttgcgc gggatcatca ttgaacggcg cggtgccgcc gccaaaaaat tcgcccgccg
     8641 caccctgaag ccacgaaccg cccgcataat cgaagacatc gccgagcgtc gccgcctggc
     8701 cgtgatgcag atacacgcgc gtggcgtgca ggccgtggag acttggtgtg tcaatgccgg
     8761 gcagcaccgc tccaagccgc gagccgctga gcgccgactg cgtgcccacg ttcatgagcg
     8821 cgttgttcgt gaaactcgtg ccgctgtggc atgtggcgca gttttgcgcg gtaaaaacgg
     8881 cctgcccggc cacggcggcg gcagtgagca ttccgttcgc atttcgatgc gggctgcgcg
     8941 gcgtgtgctc gggcgtcaaa gacgacacat aggccgccaa cgcatcgaga tcggcgctga
     9001 ggcccgtttt tccgctcgct ggcgagggat gctgcccggc gaactgctgc ggagtgagat
     9061 tcaaaaagcc cgtgccgccg aagggaccgc ggatgtcgtg ctcgaagtcc tgaatctcgt
     9121 caaaattccc gctccaatga accgcgccat gccccaggcc gctgcgacca cgcagatcgg
     9181 tggtgcggcg cagaccttcg ccgcgaccgg tgaagtccca cacacgcccg tcgtggccgc
     9241 cgtcgatgtg gcaggtggcg caggagatgt agctgtccgc gctcatgcgt ggatcggcgg
     9301 cgttgtagaa gatttgtttg ccgagcaaca cgcctgcggt gagcagctcg gtgtccaccg
     9361 ccggagtggt ggcgaccagt ggcagcgagg tttgattttg cagcaggagc ggcgcggcgt
     9421 tccgcactgt cacactgcgg cccatgaagt cctgcgtgaa gagccggttt gatgttccat
     9481 ccatcaaaac accctgcggc gccagtccgg tgccgacttc aaacgcgatg accgccggct
     9541 gcgtgatcat ggagccttga acgatctgct ccgtgagcgg cgcgaggttc agcgcgtcca
     9601 tgccgaccac gcggttgttc ccctgatgcg tcacgagcag catgtccccc agcggcgtgt
     9661 aagtcaccgc actggggctg tcgctgttgt caaagtcacg gcgcgagtgc gggatctcgg
     9721 cgttgctgtt caaatcgagg aaggagatga ccgcgcgtac ggtcgtctca tgcgtgagat
     9781 tgcccacacc gaagaaggtg ccgcgcagag tgttgtcctg cttgctcacg atggccgcac
     9841 gggtgccatc cggcgagatg gcaatgcccg cgaggtagtt cggcacaccc gccgcgcggt
     9901 cgccgccatc gagcgtgttc gccgagctga gcgtgaaggt gcgcaccgcc gtcagcaagg
     9961 cattgaactc ccgcacctcc gcgtgcaact ctggagaaat gaagcgcgtg accagcacac
    10021 gcgaaccatc cgcgctgatg gcaacggcac gcggtgtgtc catgcccgtc accgtcagcg
    10081 gcgcatcacc cggagccgcc gccgaatagc gatgcacctg cccggagccg aacatcgtga
    10141 cgtaaagcag cgtgccgtcc ggcgagggcg cgatgccatg tggcgcggag ccgtaggcca
    10201 gcgggatcgt cgcatgagcg ctgccatcgg cattcaacac gcggatttca tcgctatcat
    10261 ggcacgtcac ccagtagcgc ccgttcacat cccgcgcgat gccgcgcggg tcggcaccga
    10321 cggcatattc gtcttctttt gtgccggtga cggcattgac cacggtgacc gtattggcgt
    10381 ctggattgac gctccacact cgccgcgtgc ccgcgtcatc ccccacggcc agcgtgctgc
    10441 tttgcgtcgg acgcggtccg gcaggtggtg gtgtgatgac cagcagccgc agcgagccgt
    10501 ggacgatgct gccatgtccg tcccgcacct gcaccagcac gcgcgggcgg ccggcgctcg
    10561 tgtaggtgtg gctggcgaag ttgtccgtgt cccagtccga ccaggtgccg tcaaagttga
    10621 agcgatactc caacaccgga tcccccgtgg ccgccacggt caccgtcacc tgctgactgg
    10681 gcgcgggttg atacgcatcc gccgtcacac tgccgatcac aggcggcgcg gtggcattga
    10741 tcccgccacc agtggcaaag cggaacgagt acggctcgat gtagttcccc gccgcgtcgc
    10801 gaaagccggt ctccgtgccc ggatcactca aaaagtccac ctgatacgtc gtgtccgccg
    10861 ccagcccggc atccggcgtg aaggtcagca cgcccgcgaa gtcgtggatg agaaagccgg
    10921 gcacaaacgc gcccagcaca tccccttccc ccacggggcg cacagtgaag tcaatgccat
    10981 tgcgcggacc gcccgcgcgc gagtgctcgt gcagcagaaa gctcagcggc gcgtggcgcg
    11041 ggtaattcac gcggttcgtc tgcgggatgt ggtagctcac ctgcggcggc gcggtgtccg
    11101 gctcctgctg atgcacccac acgcccaggc cttgattcgt gccagggatt gggtagccgc
    11161 ctgtgagcca cagattgccc agtggcagcg acatctgcga ggtgtccacg cccacgatgg
    11221 ccccgcccgg cagcgcgggc gctcccggcg ggcgtggcgg gtttgtttcg ttcagcgtca
    11281 gcacgatcgg gttcgcatcg cccgccagga agcgcgtcat gtcgatcttg cggttgtgga
    11341 tgaagccgaa attgtcctga tacaccggat acgaggcatt ggtgaaattc ggcaccgtca
    11401 gactcgccgc cacatgcacg gagccatccc ccgccgggtc cgcagcctgc gtgatgtccg
    11461 cgccgatgag gatgtccgtc gtcgagccga tcacataaag ccccgtgcca tcgctgaaca
    11521 agttcggcca gtagccctgg aagccgccat ccagcgagcc gacaaagtgc ggtgtgatgg
    11581 agcggttcgc gcgcaccggg tcatcaaagt tgtgatactc caggcgatac accaccacgc
    11641 cgtcatgccc cgccacgccg ctgcgcgcca tgatcagcag atcgccaaag agcatcggat
    11701 gccagtcccc gccgccgaac tggcccacat gatccaggct ggccaccgtg ttgaaagcga
    11761 caaagcccga gcccgtcagg taggcctggc ggatgtcaat gttcgcgtcc ggcgagttgt
    11821 accacagcgc gttcacctcc cacggccccg ccatgctgcc gcggtcgtag ccgatgggca
    11881 cctggctgag ctgcgggatg ccgttcgtgc cgccgcgctc caccagcccg ccgaagccct
    11941 gcacccgcat cacctgcggc agcagcagag agtcccactg catcgtgcca tgtgcatgca
    12001 cgcccacatt gccgaagtac caaaagtcac cctcatacgc cagcccgaag tccgagggga
    12061 agcgccgcac cggattcgcc ggatcggcga tgtcatacac gcgcatcagc cagtccgacc
    12121 cgtcccgaga gcccggctgt tccgcgccca cgatgatcca gccgttgagg tagttgatcg
    12181 aagtcgtccg cccgatcggc tccgaaccgt tcgtgacggg atgcgaaagc agcgtccccg
    12241 gcgcctccgg gcggaaggcg ttgttgaagc cggtggtcgt cacctgcgcg accgcgctga
    12301 cgacaagaaa ggagaatgca aatgcgaggc ggaaaaaaat catggcagac ctttcatggt
    12361 tgaggctggc gggcggtgat aaagatggct ccgaaatatc acttttccat cacttgcgag
    12421 aagtgttctt cccgcgcgag ccttggctga gccgccggat ggcaggcaga ttccttgcag
    12481 agaggtcgaa atgccggaca ccgcgcgcgc gttcaatcca gccatgcgtt tacccagcct
    12541 gccgctttcc accctgctcg tttccctcac ttggaccgcc cgtgccgaac tgcgtgtgcc
    12601 cgccattttt ggcgatcaca tggtgcttca gcaaaagcag gccaaccccg tctggggctg
    12661 ggacacaccg ggcacggagg tgacggtgca attcgccggc cagacaaagt ccgccaaggc
    12721 cgggccggat ggcaaatgga cggtgaagct ggacccggcg cccgcgaata ccaggccagc
    12781 cacgctttcc atccagggca gcagcgcgcg ccagttccag gacgtgctcg tcggagaagt
    12841 gtgggtctgc tcagggcagt cgaacatggg cttcaccctc agcggcacct gggacgcgga
    12901 gctggacaag gcgcaggcgc ggcatccacg gctgcggctc atcaccgtgc cgcgtgtggg
    12961 cactcaggag ccgcaggacg acttcaaggg agcctggcag ccatgcactc cggaaaacgc
    13021 gggcgcattt accgccgtgg gttatcactt tgggcggatg ctgcatcaaa tgctcgatgt
    13081 tcccgtgggc ctcatcaaca acgcctgggg cggcagcgcc tgcgaagcct gggtgagccg
    13141 ggatgtgctg gaaaaagacg ggcgctttgg cgaaatgatg gcgcgctgga ggcagaccga
    13201 gcagagcttc acccaggagg gcttcgacaa gcagatggag gcgcacaagg ccaaagccgc
    13261 cgcttggctc aaagagcggc agcaggcgct gaaaagcggc gcgcgcttca ccaaggccgc
    13321 cccgcgcgcg ccgcaaaacc ccatgaccgg ccagcaccgt cccggcaatc tttacgcagg
    13381 agtgctgcac cccaccattg gctacggcat caagggcgcc atctggtatc agggcgagac
    13441 caatgccgcc cgcgcccatg aatatggcca ccttttcccc ctcatgatcc agcactggcg
    13501 cagcgaatgg aagcagggcg actttccctt ctactgggtg cagcttgccg actaccttgc
    13561 ggaaaagcca gagcccggcg acagctcatg ggccgagctg cgcgagagcc agacgaaaac
    13621 gcaaaacgcc gtcaagaacg gcggccaggc cgtcatcata gacctcggcg aggccaacga
    13681 catccacccc aaaaacaagc gcgacgtggc cgagcgcctc gcccgcatcg ccctggcgcg
    13741 cgactacggc gtggacatcc gctggcgcag ccccgagttc aaatccgccg agttccaggg
    13801 cggcaaggcg gtggtcacac tcgacacctt cggctccagc ctgcgcaccg tggacgtgag
    13861 cgaggtcaaa ggcttcgccc tctgcggcga ggaccgcaag tgggtgtggg ccggggcaaa
    13921 actcctcggc aaagacaaag tggaggtcag cgccaaaggc gtgccgaatc ccgtggccgt
    13981 gcgttacgcg tggtcggaca atccggcgtg caatcttttc agcgtcgagg gcctgcccgt
    14041 cacgcccttc cgcagtgacg acttcccgat gatcaccgcg ccggcagcgc agtgacgggc
    14101 ttattgtgat ctggcaaagg aaggcggcag gcaaaccggt tcgtttggtt ggaaaaatcc
    14161 tcaagaccac gcctcctgca gtctccttgg ctgaattaca ttccatgcag aatctgaaag
    14221 ggcggattgg agcgcgatgg cagaggccat gagatcgaaa atcaaattcg ccaagtgatg
    14281 atgcgcttga ttcatcaaga gcaaggcatg gttccgcatg tgcggagtgt gccaggactg
    14341 cagtttcact ccgttcatga tcatggtctt gagctgtccg agccataatg atgcgtcctc
    14401 accacccgct cgaacagcta ccgcgtccac ttcatcgccg agacgatcgt caaagcccgc
    14461 ggcaccgcgc ccgacacctt cgaccccgcg cgcgaccgcg tcaccgccag ccgccagggc
    14521 a
//
LOCUS       DAOMYJ010000127        14357 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Prosthecobacter sp. isolate AMR_MDS_5629
            NODE_1417_length_14357_cov_21.222137, whole genome shotgun
            sequence.
ACCESSION   DAOMYJ010000127 DAOMYJ010000000
VERSION     DAOMYJ010000127.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867850
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prosthecobacter sp. (wastewater metagenome)
  ORGANISM  Prosthecobacter sp.
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Verrucomicrobiaceae; Prosthecobacter.
REFERENCE   1  (bases 1 to 14357)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14357)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.30
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:34:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,742
            CDSs (total)                      :: 4,684
            Genes (coding)                    :: 4,676
            CDSs (with protein)               :: 4,676
            Genes (RNA)                       :: 58
            tRNAs                             :: 55
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14357
                     /organism="Prosthecobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1417_length_14357_cov_21.222137"
                     /isolate="AMR_MDS_5629"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1965333"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..803
                     /locus_tag="PLP58_13515"
     CDS             <1..803
                     /locus_tag="PLP58_13515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ09590.1"
                     /translation="FKEAAELIERMLSLHPDLDRINASNLHFHAAQCWAFAGEKDNAL
                     THLELAQHHTQVGGLLWDSYVDGTAAFLKGDKRELLNAHEHLAEKADDLNKPNLCVLD
                     RLVANFGKSYAEAYETDGQDHKKLSADCFNRAWELLDKKDRTKEDDERMVSLAHASLA
                     HWRMRDDCTDRNLSIGYWQLSRVYAVLGQGNNAERYGGLCLRISGKEPPFYLGYAHEA
                     LARAALLNERKELFERHMTEAKTLAAKVTDADEKKALEDDLAQLAWKK"
     gene            807..1352
                     /locus_tag="PLP58_13520"
     CDS             807..1352
                     /locus_tag="PLP58_13520"
                     /inference="COORDINATES: protein motif:HMM:NF024698.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HRJ09591.1"
                     /translation="MRAEAKSLHTGRLRLEPVHVAHAAEMFPLLSDTRIYEHLHFKPP
                     ATLAELEERYRRQEAGVSPDGVERWHNWIVRLEADGGACAGFVQATLHTETTGDFGYI
                     FGPAFWGRGIAFEACALALEALWADTRLARLFATVDRHNARSLALLSRLGFHRVSSKE
                     YPHGRVLPSDDIFHLPNPPPS"
     gene            1349..2260
                     /locus_tag="PLP58_13525"
     CDS             1349..2260
                     /locus_tag="PLP58_13525"
                     /inference="COORDINATES: protein motif:HMM:NF014305.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HRJ09592.1"
                     /translation="MKRLLASIFLASILSTHAREWTSSDGRKLQADFVSATATHVTLK
                     IAGKDTLVPLNRLSAADQAFVKEQATKLVVAKKIEGPFASLITGDWALSEHKNLPFAL
                     YAAADLDASKTYPLILALHGKSDNNENGLQVGGWMKSFIKEERYKKNPCIIAAPLCYQ
                     PYGGTGGGWSDKPGTEAVALVKELLKSLPIDKGRVYCVGYSMGGFGTCHLINTETRLF
                     AAGVAVAGCTGPETAGTFKKVPLWLYHAADDQTVEVKYSRDLWEELKRDKECKYTEYP
                     TGGHGIAGKVFEEATLHEWLFAKGVKP"
     gene            complement(2261..4555)
                     /locus_tag="PLP58_13530"
     CDS             complement(2261..4555)
                     /locus_tag="PLP58_13530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ09593.1"
                     /translation="MSLTGVATAQQRMEVSRDLWISAYSKEVEGNNGGSHRLKLKGIQ
                     EFFLIDFDPAPLRGKKITKAQLHVHLASPEAPLRRTTVSTISQDWVEGAGSSYAKTPG
                     ASSFVWAKTGELRWGNNEPDITSVVCGEGGSLWGFGEASAPDAEGWQVIPIAPEVAQA
                     RLEGWSHGFYVIDDIGSEYTRDGNAVKFTNFVNRYVSSREDKRIHKPYFTLWLEDGAV
                     EAPKVVNQVNPVAAATLPPVPAISAVKPTLPSKDIFGRELASTHFFAAKGETIGFSVA
                     GKALIKAPDLGVKLYSMPKVAGLTDPLVPGDGDETFIDLHVPKNTPAGDLKGTITVNG
                     TAMPFTITVWNFTLPDRLSFLPQMNAYGLPGHARDYYRLAHEHRVVLNQLPYGWTGKV
                     DEPAPKLGGDGKWDWTKFDAEFGPLLDGSAFADLPRGAVPVDAYYLLLNENWPMKHDE
                     HFKGGYWIESAFDEAYWTQFRAMAAEIAKHLEAKGWTEPMFEFYLNNKVYFKKDDWRK
                     CTAAWIFDEPVHTQDFWAIRHYGREFWNAVAPYPKVNLTYRADISRPQWQRELLDHCV
                     NVEVVSGVLRDYWPRIHRRAEVCGNLYYMYGSANAIGTPNIANAAWCVEAWSLGADGV
                     VPWNTIGKDDAWQTPDELSVLYPTANGPVPSLRLKTFRAGQQLVEYLTQYCAVSGDSR
                     ESVMAALRAIPGLSATLVKKNEEDAGKSQFGQDTQPAFEALRMRLGAYLDAKAPAPKD
                     RWHDPRPARPDLKKAREIVPLAVP"
     gene            4667..6055
                     /locus_tag="PLP58_13535"
     CDS             4667..6055
                     /locus_tag="PLP58_13535"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase"
                     /protein_id="HRJ09594.1"
                     /translation="MPRTLIFLCGLLATLQTNAAPNLLLIIADDCTYRDMEVYGGQAK
                     TPHLNKLASEGMKFTRCFQAAPMCSPTRHCLYTGLYPVKSGAYPNHTMAYDWVKSIAT
                     YLNEAGYTSHLSGKTHINPKSVFPFEYSSLKGDNNPDPAVFAEVLKSSAQNGKPFLFI
                     AASNEPHTPWNKGDPSAYPPSSIKLPPVLVDSPDTREGFSKYLAEITYFDSQVGELVG
                     LLDQSPLRDNTLVIVLSEQGNSLPFAKWTCYDAGLASACIARWPSQVKAGTVSDALVE
                     YVDVTPTFLDAAGVAQPDILDGRSFLPVLKGEATAHKTHVFGLQTTRGINAGSDFYGI
                     RSVRDGRYRYIRNLTPDAIFQNAATGDATFKTWRQMAAAGDVHARRLVHDYQHRPAEE
                     LYDTENDPWNRVNLIADPNFASVRDELRSQLDTWMKQQGDEGQATEMKALERMPRSSK
                     SEGKKKAKKGKA"
     gene            complement(6136..7476)
                     /locus_tag="PLP58_13540"
     CDS             complement(6136..7476)
                     /locus_tag="PLP58_13540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009958878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HRJ09595.1"
                     /translation="MDLLVNPTESSLLRFTTAGSVDDGKSTLIGRLLYDSKSIFEDQL
                     LAVEESSKRRGDAHVNLALLTDGLRAEREQGITIDVAYRYFATPKRKFIIADTPGHIQ
                     YTRNMVTGASTADLAIILVDARLGVIEQTMRHTYLASLLRINQIVLAVNKMDLVDFDE
                     DTYHKIIADYMAFAQGLDYTPNIRPIPISALNGDNVVERSEKTDWYDGPSLLEHLETV
                     QVHAETAADKARFPVQWVIRPISDADDSKLGNLHDYRGFAGRMASGTFRVNDEVTVLP
                     SGMRTRITGIHTFSGEQQEAIPQLSYSVTVADEIDTSRGGMIVQASEPVFTESEFDAM
                     ICWFSDKKTLKPRGRFHLRHTTNDVRAIVTDVLYKVNISTLEKSQESQEFALNDIGCV
                     RIRCSSPIFFDSYAENRITGSFILVDEQTNNTVAAGMILKPVRVEEFEDAAAAI"
     gene            complement(7516..7809)
                     /locus_tag="PLP58_13545"
     CDS             complement(7516..7809)
                     /locus_tag="PLP58_13545"
                     /inference="COORDINATES: protein motif:HMM:NF016876.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HRJ09596.1"
                     /translation="MRVQLVNGAQRDFTGGLRWYRKRSDWAADGFAEEVNNAITRILQ
                     DPARNRQIRPNVRTIRIKRYPYSLIYLIHPGTIIIVAIAHDKRRPGCWTRRLD"
     gene            complement(7806..8069)
                     /locus_tag="PLP58_13550"
     CDS             complement(7806..8069)
                     /locus_tag="PLP58_13550"
                     /inference="COORDINATES: protein motif:HMM:NF021253.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="addiction module protein"
                     /protein_id="HRJ09597.1"
                     /translation="MNATVPSPVDELLEAAKRLSNREQGEFLDRFLDLNYEGDDTDEE
                     QPLPPEWMAEIERRVAEVEAGTAILRDWQDVLEEARESLQRPA"
     gene            complement(8096..9004)
                     /gene="cysD"
                     /locus_tag="PLP58_13555"
     CDS             complement(8096..9004)
                     /gene="cysD"
                     /locus_tag="PLP58_13555"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009958879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004781 - sulfate adenylyltransferase
                     (ATP) activity [Evidence IEA];
                     GO_process: GO:0019419 - sulfate reduction [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase subunit CysD"
                     /protein_id="HRJ09598.1"
                     /translation="MSAITHLQFLESEAIYVLRETAAQFSNPALLFSGGKDSIVMAWL
                     ARKAFYPARMPFKLLHVDTGHNFPEAMTYRDWFVNEIGAQLIVGSVQKSIDEGRVQEE
                     KGPYASRNKLQTVTLLDSVEEHQFDACLGGGRRDEEKARAKERFFSHRDEFGQWDPKN
                     QRPELWNIFNGRKHFGEHFRVFPLSNWTEMDVWQYIREENIPLPTLYFAHKRRVFERH
                     GQILDAETGFIPILEEEKAHVAERLIRFRTVGDATCTGAVESSASTLDEIIAEVAAAR
                     QTERGTRADDKRSETAMEDRKKEGYF"
     gene            complement(9039..9689)
                     /locus_tag="PLP58_13560"
     CDS             complement(9039..9689)
                     /locus_tag="PLP58_13560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009958880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenosine phosphosulfate reductase family
                     protein"
                     /protein_id="HRJ09599.1"
                     /translation="MTEDQLQALNDRFLSASPREILEWALGQGNAIVTTNFRPYEAVI
                     LHLATEIQPEIPVLWVDHGTNLPETYRFAEACRQRLGLNLKPYLPLMTSAHWLALNGG
                     AVPMPDEVERVESFSRIMKLEPFERGMRELAPAVWITALRREQNPQRAATLRPVMWDA
                     KFNCLKINPILDWTPVEMEAHLTEHQLPNEKTYYDPAKGDEKHECGLHAKLVTDKA"
     gene            9804..10862
                     /locus_tag="PLP58_13565"
     CDS             9804..10862
                     /locus_tag="PLP58_13565"
                     /inference="COORDINATES: protein motif:HMM:NF021632.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase assembly
                     protein OpcA"
                     /protein_id="HRJ09600.1"
                     /translation="MPPAEDLLSRLGAEVPLPKIDRALKDLWADDETKTRASLINFAI
                     YSEAPSTLARNNNLLDKITAEHACRALLILCLPDRPQRARAWINALCRPYQGRQEVCS
                     EQISFLLEGGDAAQVQNVVFAHLDSDLPLVVWWQGGLTQNFEERFYSRIDTLIMDSST
                     WAEPAHEFARLAAVRADRGALLDVRDLSWTRSHFLRTALASCFQDANARTHLPLIEGI
                     EITHSRDQRSAALMLAGWIAHRLGAGLAEDQSGLVFTKPGGGLLKALLVEKDDGCALQ
                     ALKLNGPGIQVAVTREGASAFVHLCASCEGHAHEEMLPADVVGEAELISEQLSRAGGS
                     THYSTVLPMMEKLLARAG"
     gene            complement(10870..11862)
                     /locus_tag="PLP58_13570"
     CDS             complement(10870..11862)
                     /locus_tag="PLP58_13570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ09601.1"
                     /translation="MLAGMSGGGGRDALGALRESSAWPWHQQQMDALWRQHEAGRGQR
                     VRAWAAREIGDLQRHRALFYPFSGPDYLFAQELFPHSETYILCGLEPAEPLPDLARMS
                     AGEVAAGLQGLRTSLSSIMNAGYFITKDMRQDLQSTRFRGTLPVLLAFLARSGAVVDS
                     VDIVRLDGTGAPVLSGAAGGTAPGLMIRYRHGLGGLKRLFYFRQDLANGSMRAGSPLL
                     NFVARQGAPPVLLKSASYLMHDGRFSVIKNFILRNSAGIVQDPSGVPWRDLAASGLDL
                     RLYGDYQGTLGIFSQQPDLRAAYQSGRWPAQPVDFGFGYLFRPSNTSIIVARRR"
     gene            complement(12154..>14357)
                     /locus_tag="PLP58_13575"
     CDS             complement(12154..>14357)
                     /locus_tag="PLP58_13575"
                     /inference="COORDINATES: protein motif:HMM:NF025264.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="immunoglobulin domain-containing protein"
                     /protein_id="HRJ09602.1"
                     /translation="TATVIDSSTAYKVVVQGKNGVFVVPVISKTQTFQWYRNGNPLAA
                     SAKYVGVTSAKFTVKATNVDDAGEYECRTTIGGDTVILSRPVLIVVQPPASKVVAVGG
                     QLELEALHPYGGAMSVTPALFQYQWFKNKVLIPGAESKIYEIPTAVGNDRGAYTCMVT
                     YPSGGSGLSGVANVTVLNTNPFVVNVNAGKSAKLSLKSVLFAGKNVSFQWKRDGNPLV
                     ESLKYVGVNKSSLTITGCSLADADDYECEVSYPGSSLSATVDLVVFSAPEIVNLVFPS
                     AIVGGQFIYQIEFSPDPLYVPEKFAASPLPKGLTINKSTGLIEGVPTVSGSFDVLVTV
                     TNDVGPSTETATLVVNPLPAHLVGNFVAPLPRMPAGQFADLGGRFDMIVTATGAASGK
                     VTVGPLAFSFKGTISVIGTDGNATAEASLLAPRAGGDLTITFDLGTDDYLTDGEITGA
                     GNTVPFEGWRNTAPGAGLQGLYNFAIGLTAGDMGVDSIPQGSGYGSFTVSPTGALNIA
                     GSTSDGESITSASFVGPDGEIMFFQSLYKTVAKGSILAEGQIDDSLAGNPLGGEGTWS
                     RPENPSSTHRRYKDGFGPVDVIIAGGLYAPGANLLGATAADLTFEEGGIELASRNPNR
                     SFSVASGSTLPVPSSSTPALTVFKGVNLATGAFNGSASLADDDPTTPGVVKADEYKRT
                     LSFKGLVVPIGGELVGYGFFVIPQIPTLAPPTTTSTSPELSGSVLFEKTAP"
BASE COUNT         2897 a         4442 c         4216 g         2802 t
ORIGIN      
        1 gcttcaagga ggcagcggag ttgatcgaga ggatgctctc gctgcacccg gatctggacc
       61 gcatcaacgc gtcaaacctc catttccacg ccgcccagtg ctgggccttt gccggcgaaa
      121 aagacaacgc gctgacgcat ctcgaactcg cgcagcatca cacgcaggtc ggcgggctgc
      181 tctgggacag ctatgtggat gggaccgcgg cgtttttgaa aggcgacaag cgcgagcttc
      241 tcaatgccca cgagcacctg gcagaaaagg ccgacgacct taacaagccc aacctctgcg
      301 tgctcgaccg cctggtggcg aattttggca agtcgtatgc cgaggcgtat gaaaccgacg
      361 gccaggatca caagaaactc agcgcggatt gtttcaatcg tgcatgggaa ctgctggaca
      421 agaaggatcg caccaaggag gacgacgaac gcatggtctc gctcgcccat gcctcgctgg
      481 cgcactggcg gatgcgcgac gactgcacgg accggaacct gagcatcggc tactggcagc
      541 tttcacgcgt gtatgccgtg ctgggccagg gaaacaacgc ggagcgctac ggcgggctgt
      601 gtttgcgcat cagcggcaag gaaccgccgt tttatctcgg ctacgcgcac gaagcgctgg
      661 cacgagccgc tttgctgaat gaacggaagg agctcttcga acgccacatg accgaagcga
      721 agacgctggc ggcgaaagtc accgatgcgg atgagaaaaa agcgctggag gatgatctgg
      781 cgcagctcgc gtggaagaag tgaatcatgc gtgctgaggc aaaatcactt cacaccgggc
      841 gtctccgcct cgaacccgtg catgtggcgc atgcggcaga gatgttcccg ctcttgtccg
      901 acacgcggat ttacgaacac ctgcacttca agccgcccgc gacgctggca gagctggagg
      961 agcgctaccg gcggcaggag gcgggagttt cgccggatgg cgtcgagcgg tggcacaact
     1021 ggatcgtgcg tttggaggcg gatggcggcg cctgcgccgg ttttgtgcag gcgacgctgc
     1081 acacagagac gacgggcgat ttcggctaca tcttcggccc ggcgttctgg ggtcgcggaa
     1141 tcgccttcga ggcctgcgcc ttggctttgg aggctttgtg ggctgacaca cggctcgcac
     1201 ggttgtttgc gacggttgac cggcacaatg cgcgatctct ggcattgctc tcgcgcctcg
     1261 gttttcaccg tgtcagctcc aaggaatacc cgcatggccg cgttttgcca tcggacgaca
     1321 ttttccacct ccccaaccct ccaccatcat gaaacggctc cttgccagca tcttcctcgc
     1381 ttccatcctc tccacccacg cgcgcgaatg gacctcatcc gacggtcgca agttgcaggc
     1441 ggattttgtg tcggccacgg ccacgcatgt gacgctgaaa atcgccggga aggacacgct
     1501 ggtgcctctg aaccggcttt cggccgctga tcaggcgttt gtcaaagagc aggccacaaa
     1561 actggtcgtg gccaaaaaga tcgaaggacc cttcgcatcg ctcatcaccg gcgactgggc
     1621 gctgtcggaa cacaaaaacc tgcctttcgc gctttacgcc gccgcggatc tcgacgcctc
     1681 aaagacctac ccgctgatcc tcgcccttca cggcaagagc gacaacaacg agaacggcct
     1741 gcaggtcggc ggttggatga agagcttcat caaagaggag cgctacaaga agaacccgtg
     1801 catcatcgcc gcgccgctct gctaccagcc ctatggaggc accggaggcg gctggagcga
     1861 caaaccaggc acggaggccg tggcgctcgt caaagagctg ctcaaatctc tccccatcga
     1921 caagggacgc gtctattgcg tcggctactc gatgggcggc tttggcacct gccatctcat
     1981 caacacggag acgcgcctgt tcgccgccgg agtcgccgtc gcaggctgca ccgggccgga
     2041 aaccgcaggc accttcaaaa aggtgccgct gtggctttac cacgcggccg atgatcaaac
     2101 cgtcgaggta aaatactccc gcgatctatg ggaagagctg aagcgcgaca aagagtgcaa
     2161 atacaccgaa tacccgaccg gcggccacgg catcgcaggc aaggtcttcg aagaagccac
     2221 gctgcacgaa tggctcttcg caaaaggcgt gaagccgtaa tcacggcacc gccagcggca
     2281 caatctcgcg cgccttcttc aaatccggcc gcgcaggccg cggatcatgc cagcgatcct
     2341 tcggtgcggg tgctttggca tcgagatagg caccaaggcg catccgcagc gcttcaaagg
     2401 caggctgcgt gtcttggccg aactgcgatt tgccggcgtc ttcttcgttc ttcttcacca
     2461 aggtggcgct gaggcccgga atggctcgta gcgcggccat gacgctctcg cgactgtcgc
     2521 cgctgacggc gcagtattgg gtgagatact cgacgagttg ctgaccggcg cggaaggttt
     2581 tcaggcgcag gctgggcacc gggccgttgg cggtggggta gagcacggag agttcgtcgg
     2641 gcgtttgcca cgcatcgtct ttgccgattg tgttccaggg caccacgcca tccgcgccga
     2701 gcgaccaagc ctcgacacac cacgcggcgt tggcgatgtt cggtgtgccg atggcgttcg
     2761 cgctgccata catgtagtag agattgccgc agacctccgc gcgacgatga atgcgcggcc
     2821 agtagtcacg cagcacgccg ctgacgactt caacattcac gcagtggtcg agcagttcgc
     2881 gctgccactg cgggcgggaa atgtcggcgc gataggtcag attcaccttc ggataaggcg
     2941 cgacggcatt ccagaattcg cggccgtagt ggcggatggc ccagaagtcc tgggtgtgca
     3001 ccggttcatc gaaaatccag gccgcggtgc atttgcgcca gtcgtccttt ttgaagtaca
     3061 ccttgttgtt gaggtagaat tcaaacatcg gctcggtcca gcccttcgct tcgagatgct
     3121 tcgcgatctc cgccgccatc gcgcggaact gcgtccagta ggcctcatca aacgcactct
     3181 cgatccagta accgcctttg aagtgctcgt cgtgcttcat cggccagttc tcgttcagca
     3241 gcaggtaata cgcgtccacc ggcaccgcgc cacgcggcag atcggcgaag gcgctgccat
     3301 cgagcagcgg gccgaactcc gcatcgaact tcgtccaatc ccacttccca tcgccgccga
     3361 gcttcggcgc gggctcgtcc accttgcccg tccagccata cggcagttga ttcagcacca
     3421 cgcgatgctc atgcgcgagc cggtagtagt cacgcgcatg ccccggcagg ccgtaggcgt
     3481 tcatctgcgg caggaatgac aagcgatccg gcagcgtgaa gttccaaacg gtgatcgtga
     3541 acggcatagc ggtgccattc actgtgatgg ttcctttcaa atcgcctgcg ggcgtgtttt
     3601 tcggcacatg caggtcgatg aacgtctcat cgccatcacc cggaacgagc ggatcggtca
     3661 atccggccac ttttggcatc gagtagagct tcacgcccaa atcaggcgct ttgatcagcg
     3721 ccttgcccgc gaccgaaaac ccgatcgttt cgcccttcgc ggcaaaaaag tgcgtcgagg
     3781 ccagttcgcg gccgaaaatg tctttcgaag gcaaagttgg cttcacggcc gaaatggccg
     3841 gaacaggcgg caaagtggct gcggcgaccg gattgacctg attgacgact ttcggtgcct
     3901 ccaccgcgcc gtcttcgagc cagagcgtga agtagggctt gtgaatgcgc ttgtcctctc
     3961 gactcgaaac gtagcgattc acgaaattgg tgaacttcac cgcgttgcca tcgcgtgtgt
     4021 attcgctgcc gatgtcgtcg atgacgtaaa agccgtgtga ccagccctca agccgtgcct
     4081 gcgccacttc gggcgcaatg gggatgacct gccagccctc cgcatccggc gcggaggcct
     4141 cgccaaagcc ccacagcgag ccgccttcac cgcagacgac gctggtgatg tccggctcgt
     4201 tgttgcccca gcgtagctcg cccgtcttcg cccatacaaa ggaactcgcg cccggtgtct
     4261 tcgcgtagct gctgccggcg ccttccaccc agtcctgcga gatcgtggag accgtcgtgc
     4321 gccggagcgg tgcctccggc gacgcgagat gcacatgcag ttgagctttg gtgatcttct
     4381 tcccgcgcag cggcgccgga tcaaagtcga tgaggaagaa ctcctggatg cccttcagct
     4441 tcagccgatg cgatccacca ttgttcccct ccacctcctt cgagtaagcc gagatccaca
     4501 aatcccgcga cacctccatt cgctgctgtg ccgtggcgac acctgtcaaa ctcatcaaac
     4561 caaggaaaac gaatcgagtc atcggtcgtc actacgattc caagcccgtt caccttgcta
     4621 atcggtcgcc tgcaatgtct tccctgcctc cacgttcaca gtctccatgc cccggactct
     4681 cattttcctt tgcggattgc tagcgaccct gcaaaccaat gccgcgccaa acctgctgct
     4741 catcatcgcc gatgactgca cttaccgcga catggaggtc tatggcgggc aggccaaaac
     4801 gccgcacctg aacaagctcg ccagcgaggg catgaaattc acccgctgct tccaggccgc
     4861 gcccatgtgc tcacccacgc ggcactgcct ctacactggc ctttatccag tcaaatccgg
     4921 cgcctatccg aatcacacaa tggcctacga ctgggtcaaa agcatcgcca cctacctgaa
     4981 cgaggcaggc tacaccagcc atctctccgg caaaacccac atcaatccca agtcggtttt
     5041 ccccttcgaa tactccagcc tcaaaggcga caacaaccca gatcctgccg tcttcgccga
     5101 agtgctcaaa tccagcgcgc aaaacggcaa gcctttcctc tttatcgccg cctccaacga
     5161 gccgcacacc ccgtggaata aaggtgatcc ctccgcctac ccgccctcct ccatcaagct
     5221 gccgcctgtg ctggtggact cgcccgacac gcgcgagggc ttttccaaat acctcgccga
     5281 gatcacctac ttcgactccc aggtcggcga actcgtgggc ttgcttgacc aaagcccgct
     5341 tcgcgacaac acgctcgtca tcgtcctcag cgagcagggc aacagccttc cattcgccaa
     5401 atggacctgc tatgatgccg gtctagccag cgcctgcatc gcccgctggc cctcccaggt
     5461 caaagcggga accgtcagcg acgcgctcgt cgagtatgtg gacgtcaccc ccaccttcct
     5521 tgatgccgcc ggtgtcgcgc agccggacat cctcgatggg cgcagcttcc tgcccgtgct
     5581 caaaggagaa gccaccgcgc acaaaacgca tgtcttcggc ctgcaaacca cgcgcggcat
     5641 caatgccggc agcgactttt acggcatccg cagcgtgcgc gacggtcgct accgctacat
     5701 ccgcaacctc accccggacg ccatctttca aaacgccgcc accggcgacg ccaccttcaa
     5761 aacttggcgg caaatggccg ccgccggaga cgttcatgcc cgacgcctcg tccacgatta
     5821 ccagcaccgc cccgccgaag aactctatga caccgagaac gacccatgga accgagtcaa
     5881 cctcatcgcc gaccccaact tcgcctccgt gcgcgacgaa ctccgctccc aactcgacac
     5941 ctggatgaag cagcagggcg acgaaggcca ggccacagaa atgaaggcgt tggaacgcat
     6001 gcccaggagc agcaaaagcg agggcaaaaa gaaagcgaag aagggcaagg cgtgagtgga
     6061 cacattccct gatttctcct gcggatgcaa aggacaggac gagactgcgc ggagtgacaa
     6121 ataaaaattt tgggatcaaa tcgccgcggc ggcgtcctcg aattcctcca cccgcaccgg
     6181 cttcaagatc atgccggcgg caacggtgtt attcgtctgc tcgtccacga ggatgaagct
     6241 gccggtgatg cgattctcgg catacgagtc gaagaaaatg ggggaggagc agcggatgcg
     6301 cacgcagccg atgtcgttga gggcgaactc ctggctctcc tgggacttct ccagggtgct
     6361 gatgttcact ttgtaaagca cgtcggtgac gatggcgcgc acgtcgttgg tggtgtggcg
     6421 cagatggaag cggccgcgag gcttgagggt ctttttgtcg ctgaaccagc agatcatggc
     6481 gtcgaactcg gactcggtga agacaggctc gctggcttgg acgatcatgc cgccgcggct
     6541 ggtgtctatc tcatccgcca cggtgacgct gtagctgagc tgcgggatgg cctcctgctg
     6601 ctcgccggag aaggtgtgga tgccggtgat gcgcgtcctc atgccggagg gcagcacggt
     6661 gacctcgtcg ttcacgcgga aggtgccgct ggccatgcga ccggcgaagc cgcggtagtc
     6721 gtgcaggttg ccgagcttgc tgtcatccgc gtccgagatg gggcggatga cccattgcac
     6781 ggggaagcgg gccttgtcgg cggcggtctc ggcgtggacc tggacggtct ccagatgctc
     6841 cagaaggctc gggccgtcat accagtcggt cttttcggag cgctcgacga cgttgtcgcc
     6901 attgagcgcg ctgatgggga tggggcggat gttcggcgtg tagtccaggc cctgggcgaa
     6961 ggccatgtag tcggcgatga ttttgtgata tgtgtcctcg tcgaagtcca cgagatccat
     7021 cttgttcacc gcgagcacga tctggttgat gcgcagcagg ctggccaggt aggtgtggcg
     7081 catggtctgc tcgatgacgc cgaggcgcgc gtccacgagg atgatggcga ggtccgccgt
     7141 ggaggcgccg gtgaccatgt tgcgcgtgta ttgaatgtgc cccggcgtgt cggcgatgat
     7201 gaacttccgc ttcggcgtgg cgaagtagcg gtaggccacg tcaatggtga tgccctgctc
     7261 ccgctcggcg cgcaggccgt cggtgagcag cgccaggttc acatgcgcgt caccgcgccg
     7321 cttcgagctt tcctcgacgg ccaggagctg gtcttcgaag atggatttcg agtcgtaaag
     7381 caggcggcca atgagcgtgc tcttgccatc atccacgctg ccggcggtgg tgaagcggag
     7441 aagggaggat tcggtgggat tgacgaggag gtccatgagg tattgggggc ggtggatgcg
     7501 ggcggatttg aaggctcagt cgagacggcg ggtccagcag ccggggcgac gtttgtcgtg
     7561 tgcgatggcg acgatgatga tggttcctgg gtggatcagg tagatgaggc tgtaggggta
     7621 gcgtttgatg cggatggttc ggacattggg ccggatctgt cggttgcgag caggatcctg
     7681 gaggattcgg gtgatggcgt tgttgacttc ctcagcaaag ccgtccgcag cccagtcact
     7741 tcgcttgcga taccagcgca agcccccggt gaagtccctc tgagcgccgt tgaccaactg
     7801 gactctcatg cggggcgttg gagtgattct cgtgcttcct caagaacatc ctgccagtcg
     7861 cgaaggatgg cggttccagc ttcgacttcg gccacccggc gctcgatctc ggccatccat
     7921 tcggggggga gaggctgctc ttcatcggta tcgtctccct cgtagttcag atcgagaaag
     7981 cggtccagga actcgccttg ctcacgattg gacaggcgct tcgcggcttc caagagttcg
     8041 tcaacggggc ttgggacggt ggcgttcatg ggcaaatgat aacataagat tgaaatcaga
     8101 agtacccctc ctttttccgg tcctccatgg ccgtctcgct gcgcttgtcg tcggcgcggg
     8161 tgccgcgctc ggtctggcgg gcggcggcga cttcggcgat gatttcatcg agcgtgctgg
     8221 cgctgctttc cacggcgcct gtgcaggtgg cgtccccgac ggtgcggaag cggatgaggc
     8281 gctcggcgac gtgggctttc tcctcctcca aaatcggaat gaagccggtc tcggcatcca
     8341 ggatctggcc gtggcgctcg aagacgcggc gtttgtgcgc gaagtagagg gtgggcagcg
     8401 ggatgttttc ctcgcggatg tactgccaca cgtccatctc ggtccagttg ctcaggggga
     8461 agacgcggaa gtgctcgccg aagtgtttgc gaccgttgaa gatgttccaa agctcgggcc
     8521 gctggttttt tggatcccac tggccgaatt catcgcggtg gctgaagaag cgctctttgg
     8581 cgcgggcctt ttcctcgtcg cgacgaccgc cgccgaggca ggcgtcgaac tggtgctcct
     8641 cgacgctgtc gagcagggtg acggtttgca gcttgttgcg gctggcgtag gggcctttct
     8701 cctcctgcac gcggccctca tcaatgctct tctgcacgct gccgacgatg agctgcgcgc
     8761 cgatttcgtt gacgaaccag tcgcggtagg tcatggcctc ggggaagttg tggccggtgt
     8821 ccacatgcag cagcttgaag ggcatgcgcg cggggtagaa ggccttgcgc gccagccagg
     8881 ccatgacgat ggagtctttg ccgccggaga aaagcagggc gggattggag aactgggcgg
     8941 cggtctcgcg caggacgtag atggcctcgg actcgagaaa ttgcaggtgc gtgatggcgg
     9001 acatggatcg gaaactggca gggacgaatg gcgagaaatc aggctttgtc agtcaccagt
     9061 ttggcgtgca ggccgcactc gtgcttttcg tcgcccttgg cggggtcgta gtaggttttt
     9121 tcgttgggga gttggtgctc ggtgaggtgg gcctccatct ccacgggggt ccagtcgagg
     9181 atggggttga tcttcaggca gttgaacttt gcatcccaca tcacgggccg gagcgtggcg
     9241 gcgcgctggg gattttgctc gcggcgcagg gcggtgatcc acacggcggg ggccagctcc
     9301 ctcatgccgc gctcgaaggg ctccagcttc atgatgcggc tgaaggattc cacgcgttcc
     9361 acttcgtccg gcatcggcac cgcgccgccg ttgagcgcca gccagtgggc gctggtcatg
     9421 agggggaggt agggcttcaa attcagcccc aggcgctggc ggcaggcttc ggcaaagcgg
     9481 taggtctccg gcaggttcgt gccgtggtcc acccagagca cgggaatttc cggctggatc
     9541 tcggtggcca ggtgcaggat cacggcctcg taggggcgga aattcgtggt cacgatggca
     9601 tttccctgcc ccagcgccca ttcgaggatt tcacgcgggc tggcggataa aaagcggtcg
     9661 ttgagagctt gaagttgatc ttcggtcatg attcacccct atttccgata aattcactat
     9721 gcaatggcga attttgccaa aaatgccccg tcccgccggc aaactccgcc tttactcaat
     9781 tccaggggaa tctaatctgc tcaatgcccc ctgctgaaga cctgctttcc cgcctcggtg
     9841 ccgaggtgcc tttgccgaag attgaccgcg ccctcaagga tctgtgggcc gacgacgaga
     9901 ccaagacgcg cgcatcgctg atcaacttcg ccatttacag cgaggccccc tccaccctgg
     9961 cgcggaacaa caacctgctc gacaaaatca ccgccgagca cgcctgccgc gcgcttttga
    10021 tcctttgcct gccggacagg ccccagcgcg cgcgcgcctg gatcaacgcg ctctgccgcc
    10081 cctatcaagg ccgccaggag gtgtgcagcg agcagatttc ctttctgctc gaaggcgggg
    10141 atgccgcgca ggtgcaaaac gtcgtcttcg cccatctcga ctcggatctg cccctcgtcg
    10201 tctggtggca gggcgggctg acgcagaatt tcgaggagcg cttttacagc cgcatagaca
    10261 cgctcatcat ggacagcagc acctgggcgg agcccgcgca tgagttcgcc cgcctggccg
    10321 ccgtgcgtgc ggaccgcggc gcgctcctgg atgtgcggga cctaagctgg acgcgcagcc
    10381 attttctgcg caccgcgctg gcctcctgct tccaggacgc gaacgcgcgc actcacctgc
    10441 cgctcatcga gggcatcgag atcacccact cccgggacca gcgcagcgca gcgctcatgc
    10501 tcgccgggtg gatcgcccac cgcctcggcg ccgggctggc ggaggaccag agcggcctag
    10561 ttttcaccaa gcccggcggc ggcttgctga aagccctcct ggtcgaaaaa gacgacggct
    10621 gcgcgctcca ggcattgaag ctgaacggcc ccggcatcca ggtcgccgtc acccgcgagg
    10681 gcgcctccgc cttcgtccac ctgtgcgcct cctgtgaggg ccacgcgcac gaggaaatgc
    10741 tgcccgccga tgtcgtcggc gaggccgaac tcatctccga gcagctcagc cgcgccggcg
    10801 gcagcaccca ctactccacc gtgctgccca tgatggagaa actgctcgcc agggccgggt
    10861 gaagccccct caccgccgcc gcgccacaat gatgctcgtg ttcgacgggc ggaaaagata
    10921 gccgaagccg aaatccaccg gctgcgccgg ccagcgcccg ctctggtagg ccgcgcgcag
    10981 gtccggctgc tggctgaaga tgcccagagt gccctggtag tccccataca gccgcaggtc
    11041 cagcccggag gccgccaggt cccgccacgg cacgccggaa ggatcctgca cgatgcccgc
    11101 cgaatttcgc aggatgaagt ttttgatcac cgaaaagcgc ccgtcatgca tcagatagga
    11161 cgcgctcttc aacaacaccg gcggcgcgcc ctgccgcgcc acgaagttca gaagcgggct
    11221 gcccgcgcgc atgctgccat ttgccagatc ctgccggaaa taaaacagcc gcttcaagcc
    11281 ccccaacccg tgccggtagc ggatcatcag ccccggcgcc gtcccgcccg ccgcgccgct
    11341 gagcaccggc gcacccgtgc cgtccagccg cacgatgtcc acactgtcca ccaccgcccc
    11401 gctgcgcgcc aggaaggcca gcaacaccgg cagcgtgccg cggaaacgcg tggattgcag
    11461 atcctgccgc atgtctttcg tgatgaagta gcccgcgttc atgatgctgc tcagggaggt
    11521 gcgcagcccc tgcaggcccg ccgccacctc gcccgcgctc atgcgcgcca gatccggcag
    11581 cggctccgcc ggctccagcc cgcacaggat ataagtctcc gaatggggaa acagctcctg
    11641 cgcaaacaga taatccggcc cgctgaacgg ataaaaaagc gcccgatgcc gttgcaggtc
    11701 tccgatctcc cgcgccgccc acgcccgcac cctctgcccg cgccccgcct catgctgccg
    11761 ccagagcgcg tccatctgct gctggtgcca gggccatgca gaggactccc gcagcgcccc
    11821 aagcgcatcc cgcccgccgc cgcccgacat cccagccaac atccgcgcca ccgcattgat
    11881 cgagctagac tgccacggca cattaagttc cctcgctccg gaaaaaagac tgccaccgcg
    11941 tgccgactcc gctctcctga cagtcatctg gctgagtcca tgataactgt cacatgatat
    12001 cagaacaaat agagccaaga atgactggaa aaacagtcgt ttcaaaaggg cgctttgatt
    12061 catgaactta attcatacct tcccaaaaca tatgtaaaaa acaaaaactc cccgccattt
    12121 ctgaagggga gcctttggtt cattcaattc gagtcaagga gccgtctttt caaacaacac
    12181 agaacctgaa agttccggag atgtggatgt tgtagtgggc ggtgcaagcg tgggtatctg
    12241 aggaatcacg aagaaaccgt agcccacaag ttctccccct attggaacca ccaaaccttt
    12301 gaaactcaga gtgcgtttgt attcatcagc cttaacgaca ccaggagttg tcgggtcatc
    12361 atcagccagg gaagcgctgc cgttaaaagc tcctgtcgcc aaattaacac ccttaaacac
    12421 tgtgagagcg ggtgttgacg aactgggaac aggcaatgtg cttccagatg cgactgagaa
    12481 acttctattc gggttacgag acgcaagctc gattccacct tcctcaaaag tcaaatcagc
    12541 agcagtggct ccgagaaggt ttgcaccagg tgcgtaaagc ccgcctgcga taatcacatc
    12601 cacaggccca aaaccatcct tgtagcggcg atgggtggag gagggatttt caggacggga
    12661 ccaagttccc tcgccaccca gcggatttcc ggcaagggaa tcatcaattt gcccttccgc
    12721 cagaatggag ccttttgcca cagtcttgta aagagactgg aaaaacatga tttcaccatc
    12781 tggacccaca aaagaggcgc ttgtgattga ttcaccatcc gaagttgaac ctgcaatatt
    12841 caaagcccca gttggagaca ctgtgaaact gccatatccc gatccttgag gaatggaatc
    12901 aacccccatg tcaccagcag tcagtccgat ggcaaagttg tagagacctt gcagacccgc
    12961 gccaggcgct gtgtttctcc aaccttcgaa tggcacagtg ttacccgcgc ccgtgatctc
    13021 accatccgtc aggtaatcat cggttccaag atcgaacgtg attgtcaagt ccccaccggc
    13081 tcttggtgcc aataggctcg cctcagcagt ggcgttgcca tctgttccta tgacagatat
    13141 ggttcccttg aaactaaatg ccagaggccc gactgtcact ttgccagacg cggcacctgt
    13201 tgcggtcaca atcatgtcaa atcgtccacc gaggtccgca aactgccccg caggcatgcg
    13261 cggcaaaggt gccacaaagt ttccaaccag atgcgccgga agtggattga ccacgagagt
    13321 ggcggtttct gttgaaggtc cgacatcgtt agtcacagta acaagaacat caaagcttcc
    13381 cgagacggtt ggcacgcctt cgattaatcc cgtgcttttg ttgatagtca atcctttagg
    13441 aagcggggat gccgcaaatt tttccggaac ataaagaggg tcaggactga attcgatttg
    13501 ataaatgaac tgacctccga caattgcgga aggaaagact aggttcacaa tttctggtgc
    13561 cgagaacaca accaaatcca ctgtggcgct taatgacgaa ccaggataac tcacttcgca
    13621 ttcatagtcg tcagcgtctg ccagcgagca accagttata gtgagagatg acttattcac
    13681 tcccacatat ttaaggcttt caacgagcgg attgccatca cgcttccatt ggaatgaaac
    13741 atttttccca gcgaatagca cagattttaa agacagcttg gctgacttcc ctgcattcac
    13801 attgacaacg aatggattcg tattcagcac cgtcacattg gcaactccac tcaaccctga
    13861 tccgcctgat gggtaggtca ccatgcaggt gtatgcgcca cggtcatttc ccactgcagt
    13921 tggaatttca tatatcttgc tctccgctcc agggatcaat actttgtttt taaaccattg
    13981 atattgaaaa agcgctggag tgaccgacat ggcgccacca taagggtgaa gggcttccaa
    14041 ctccaactga ccaccgactg ccactacttt ggaggcgggc ggttgcacca cgatcaaaac
    14101 aggccttgag agtataaccg tatctcctcc aatggttgtt cggcattcat attctccagc
    14161 atcgtcaaca ttggttgctt tgacagtaaa tttggcactc gtaactccaa catatttggc
    14221 actcgctgcc aaggggtttc catttcggta ccattggaat gtttgtgttt tactgatgac
    14281 aggcacaaca aacacaccat ttttgccctg aaccaccact ttgtaggctg tcgagctatc
    14341 aatgacagtt gcagttc
//
LOCUS       DAOMYJ010000128        14284 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Prosthecobacter sp. isolate AMR_MDS_5629
            NODE_1426_length_14284_cov_22.228126, whole genome shotgun
            sequence.
ACCESSION   DAOMYJ010000128 DAOMYJ010000000
VERSION     DAOMYJ010000128.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867850
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prosthecobacter sp. (wastewater metagenome)
  ORGANISM  Prosthecobacter sp.
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Verrucomicrobiaceae; Prosthecobacter.
REFERENCE   1  (bases 1 to 14284)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14284)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.30
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:34:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,742
            CDSs (total)                      :: 4,684
            Genes (coding)                    :: 4,676
            CDSs (with protein)               :: 4,676
            Genes (RNA)                       :: 58
            tRNAs                             :: 55
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14284
                     /organism="Prosthecobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1426_length_14284_cov_22.228126"
                     /isolate="AMR_MDS_5629"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1965333"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(114..1133)
                     /locus_tag="PLP58_13580"
     CDS             complement(114..1133)
                     /locus_tag="PLP58_13580"
                     /inference="COORDINATES: protein motif:HMM:NF019845.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HRJ09603.1"
                     /translation="MKSVIAIHPGHLEILDTPVPVPGPYQALVRTERACICNRTDSEL
                     LHGRFPGMEDRFPFALGHESVGVVVEAGGKARSFAVGDRVVGGLVFDLQMEGVDSGWG
                     GFGEFTLATDHETMVADGVADEAHGWVEVHEIQTRVDADIPPEEAVLLCTWREVLGAF
                     RDFHLQPGDDVLIYGAGPVGLSFVKLGRLFGLGWIGIVDRHEEKRQKALALGANAAFA
                     PGQAAPPRKLDAVVDAVGSSKILLEGLPLLRRGGSHCIYGVLTEPVLHIEKARADFNF
                     NLLVHQWPTRRFEKECQPQLCQWIREGRLTSAEFITHRFPLGQIREAFDEVGRRTVIK
                     ALIEY"
     gene            1207..2004
                     /locus_tag="PLP58_13585"
     CDS             1207..2004
                     /locus_tag="PLP58_13585"
                     /inference="COORDINATES: protein motif:HMM:NF013757.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HRJ09604.1"
                     /translation="MKSTLEDHAAIRLDDNSAAPGTERTLAILELLGRHRLGLNLTEI
                     ARELDLPVNSVFRITGVLLARGYLQRREDDRRFVLTNKLFDLARPQVREKSLVVCALE
                     ALKELRDRTGETVQLLTAVNHKMTVLEQCVSSQAIKVSSTVGLRVPMYSCAPGKAVLA
                     FLPPAELEAFFAAVELKQFTPNTLATRESLQAELARSRKRGYATDLAEGLEGIHCAAA
                     PVLDDHHYPVAAITVMAPAFRLKKDRFAELGRACVEAAETIRRRLLA"
     gene            2109..5807
                     /locus_tag="PLP58_13590"
     CDS             2109..5807
                     /locus_tag="PLP58_13590"
                     /inference="COORDINATES: protein motif:HMM:NF019223.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1553 domain-containing protein"
                     /protein_id="HRJ09605.1"
                     /translation="MLSALLCTPSPAATPAQIEFFEARIRPVLAQECYECHSESGKRK
                     GGLLLDSRPGWQEGGDSGPAIVPGDAASSLLIQSIRHAHSDLQMPKNGAKLDVNIIAD
                     FERWITEGAPDPRDTAPTPEQLRADTDWNAVLQRRKQWWCFQPLQTQPPAKPLSPEQL
                     AAEIDRQLDEKSHAAGLTPAQPADNATLHRRLSYMLTGLPPDPSHPPDPTALLTSQAC
                     AEKWSRHWLDWLRYAETHGSEGDTPIPYAWRYRDYVIRAFADDVPYFQMVREAVAGDL
                     LPNPRIKDGVNESALGIGQLRMVLHGFSPTDSLDELVTWTDNQIETVSKAFQALTVSC
                     ARCHDHKFDAISQADFYALYGILTSTRPAIIDVNAPGIGEAERADLQHLKKQIQSAVA
                     RAWMKALPEKTEGGESPITLPATTHHWDLRKEKNWFTDGNGLRQGATAPGEFSIALEG
                     GRVIANLHPGGLFTDLISTADRAVLMSPRFRCEGGTLWFRVAGGGGAVAKYVVQNYPR
                     TGTIHKARELKTDRDAVLGWHKLDLEYWKGDDIHIELATAADRPAQAEFDARSWFGIT
                     EAFITHSSDNPRGPGIPSKPGQDAVRAWLAGTLTDGQAEALNRALQSGQLPNQLSAIP
                     EAAALVEKYRLLEAKLPRPTRAPGVLEGDARDAALFVRGNHKQPADLVPRRFLDGINP
                     VPFETKQSGRLELAAHLTDPQNPLTARVIVNRLWHHVFGRGLVATTDNFGRLGQTPTH
                     PELLDFLAAQFIADGGSMRRFIHALVSTRAFARSASASAADLARDPDNLHLARWTVRR
                     LEAEAIRDSILHLSGKLDATPFGQPVPGTAPRRSVYVQVIRNQLDPFLTAFDMPVPSA
                     PRGARDVTNVPAQSLALLNDPAIQTWAADWAARTETQLAPEQRVRLMFQQALAREPEP
                     NELQASLRFVESHLTEARARQDRIIALRRQVEVLLASVRSVGSVRSAPSKVLAPLAEW
                     TFESDLTDTQGRLPLTLSGAARLENGALVLDGSSMAQTGSLPKTLTAKTLEAWVQLDN
                     LTQRGGGVITVQGKDGVVFDSIVFAEKQPGHWVAGSDHFMRSEPFNCPAETEAANRIV
                     HLAVVYEADGTVRGYRDGEPYGRAYRKAPGAVFEAESSQILLGCRHGKPSGNRGLAAR
                     IHRARLYDRALTEEEIAQTARLENLPVTDHALLSALPPEQRAQVQKLRAELQNLEAQA
                     PNESTPEATAWQSLALSLLNLKELIYLR"
     gene            6004..6462
                     /locus_tag="PLP58_13595"
     CDS             6004..6462
                     /locus_tag="PLP58_13595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ09606.1"
                     /translation="MIPLPPDFSDFIRLLNRHRVRYVVVGGYAVAFHGYPRYTGDIDF
                     FVEKSARNSVALQKVFRDFGFVPPPEASLFLEDKRIVRIGFPPMRVEVSNHIDGVTFA
                     ECHENRIQARVAGIRVPFIDLERLRINKQACGRPKDMEDLRQLFGEAPQD"
     gene            6494..7897
                     /locus_tag="PLP58_13600"
     CDS             6494..7897
                     /locus_tag="PLP58_13600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009963616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1501 domain-containing protein"
                     /protein_id="HRJ09607.1"
                     /translation="MNRRHLLARASNGFGLAALAGLMAENAPAAVVTHRSKRFIPRAR
                     NVIFCYMSGGVSHIDSFDPKPLLGKYAGKPPPVPTARTQFNNNGVVQPSHWAFHRHGQ
                     SGLPVSELFPHMASVADELCVIRSMTAKFSEHAQGNFFMHTGFPFLGYPSAGAWTSYG
                     LGTESRDLPGYVVLQSGGALAPHGGVGLFGNGFLPAQHQASIIRVDETEPVRNIRPRE
                     AAVLQRRRLDFIGELDQGFLGELGAHREVEAAIQNYEMAWRMQSAVPDLCDLSGETEA
                     TKKLYGMDGPDSTRKAYGMQCLLARRLVERGVRFIELSCLPQQLGAGQAPNPWDQHGQ
                     IKEGHGNMAWQVDQPIAGLIKDLKSRGLLDSTLVVWAGEFGRTFFAQGSDGRDHNPYG
                     FSLWMAGGGVRGGMTLGETDDLGYHAVEKVQTVYDLWATVLYLLGVDHEQLTYLFSGR
                     NFRLTDVHGHVMREILI"
     gene            8004..10919
                     /locus_tag="PLP58_13605"
     CDS             8004..10919
                     /locus_tag="PLP58_13605"
                     /inference="COORDINATES: protein motif:HMM:NF013862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HRJ09608.1"
                     /translation="MNDTSSDQVPAHHLWVELSTRTTAETAHQHSGDEEAAMRSVHFL
                     SGKTRELMAGHPAAMEFNRLAERLLNEMLRPCMASWHGWMTPDPNLRDKDGQPVAKFR
                     DPQARQLFRAELGDLHGRLLAWQRVFDLLRRGLARDEQGVSLDDWSRPTNQRLALLAN
                     PDDKPAKSGPNLGAVLPLGIGGQVEVAGDVKMSEIAKAEIAEIHHRRAVLKLVRPEDK
                     KSAVWNVAGLALSGGGIRSATFCLGITQMLARHGFLHRFDYLSTVSGGGYLGAFLSTA
                     LGTRQEGDKSRTTPESAADKLKDVFERTEGIESGLVRHLRNNSKYLLNGGLGAKLEIT
                     GLLISGVLWNLLMLLPLPLILAWVAHVGQEWIWGAQIVSGSFKEPDWAKGCAADLAIL
                     FGGVLALGWFTQPLVRMLSHGRDPKDKSMLARRALEVALPWVGLAFAFMVFVNCVPTL
                     FYGYEILRQEYKALGLQWLKGTLSADVLGVSAGGAFSAALALVVAKLAPHRERLRRLA
                     VRLLVLSGPLLLLAIFLAVGNRMGLGRTVVEPEVVPVFVTMIPTVLAQYSQEYLADLS
                     TKWSPAWVLTVAIGIVLWGWLGVNINTLAPHIYYRNRLCDCYMVRRIEPDAEKPGLWT
                     RLVRRLSGEKVHGRTETLQRVRFSSLNSDLAAPYHLVNMTLNAPSSTNKNLRGRGSDF
                     FVASRRYCGSPLTGYKKTDEITSVDPHFDLGTAMAVSGAAASTSMGWKSLPNFRFLMT
                     LFNVRLGYWLRWGGKPGLWHRLANTPGPVYLFREMFAVMDERCRYLNLSDGGHIENLG
                     VYELLRRQAKFIVCVDGGQEPGMECSDLTRLQRYAEIDLGIRLHFDLHDLSPMPDGCS
                     RAYGLLVKVDYEPGESRPAAEAKVGWMLYLKLAMTGAEPAHVLDYKRDHPDFPHQTTG
                     DQIFDEAQFEAYRQLGECAMRSFFHDKLFDAGADTSLDDWFHHLACRLLPDNDDAYG"
     gene            complement(11011..11532)
                     /locus_tag="PLP58_13610"
     CDS             complement(11011..11532)
                     /locus_tag="PLP58_13610"
                     /inference="COORDINATES: protein motif:HMM:NF012436.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin"
                     /protein_id="HRJ09609.1"
                     /translation="MTLDPKLTKLLNEQINNEMASSYTYLAMSAWFEQTPYSGFASWM
                     FNQSREETMHALKFYQYVVDRDAVVELEPISKPKHKFKSPLDVFEQSLKQEQLVTKQI
                     NDLYDVAEQVKDHAAKNLLLWFLNEQMEEEKTVRDMLDRLKLAGNDPASLLVLDREAG
                     QRQGAASKNEAAE"
     gene            11716..13095
                     /locus_tag="PLP58_13615"
     CDS             11716..13095
                     /locus_tag="PLP58_13615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007415165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1501 domain-containing protein"
                     /protein_id="HRJ09610.1"
                     /translation="MFTFTDKGSVTTCDGVTRRDFLSAGTLSAIGLTLPSFAKLKAEG
                     KVDPKKSNKACIMIFNLGAPSQQDLWDMKPDAPSEIRGPFKPIRTKSNAFEISEILPM
                     HAKIADKFSLVRSCYHTAAAVHDTGHQMMQTGRLFTGGVITPHVGSALEFLQGRRSDL
                     PAHIILPETMGPTGGNMPHGQDAGFLGKAYDPFVLNANPSEKDFKVPDLLPPKEISEV
                     RLERRKQLRELVDSSVRNFEYSEQAKLMDSNFESAYRIMTSTQAREAFDLTKEPQKVR
                     ERYGMNRFGQSCLLARRLVERGVRFVTVNTFITVFGEITWDIHGSKPFTSIEGMRDIV
                     APMYDQGYSALIEDLLQRGMLDDTMVTCLAEFGRTPKINPAGGRDHWPNCWTVNFAGG
                     GVKGGEVIGKSDDIGGYPTERPVAPKEIVATIYQAMGIDLHTELPGPQGRPFPVVDFG
                     TQAIKELFA"
     gene            13143..14159
                     /locus_tag="PLP58_13620"
     CDS             13143..14159
                     /locus_tag="PLP58_13620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ09611.1"
                     /translation="MNSVPQTRRKWELGIAGLLILVTAVCSGWMCGIFGNVPPYPQEF
                     HDQEVRRRIETANKLQEQSIPRGEVHYPWYNWEPQQLDTLRDRLSWESGKISIQPVAY
                     MNGVEPPKPGQISVFLINDTDEQLQVPAQDGDIYLKLEVNLEGKWQRAQCHGDSWCGN
                     SYGTRTLAAHSYVIIQGYQPTPGRRAKVRYAIHNGASDRLTSAKFDGLYAPDDVAMSA
                     YDSLAMREAELSKLRKVLLRQDTPTLMPGSYTIDMMRRSAWYGILRGGHDRDAALKIA
                     EEVLAYDPTLEPRKADLGQLMDSWHAALEHETRGRVMIHGFTVSKRLVQNTSHQAHQN
                     NGTP"
BASE COUNT         2782 a         4768 c         4084 g         2650 t
ORIGIN      
        1 gccaggagca cgatggcgag aggggcccat agcaggggca gggagcggcg gttcggggaa
       61 atcatggacg accagaataa aggcggatac gccgcgcgca acctccgcgc cggtcaatac
      121 tcgatgagcg ccttgatcac cgtgcgccgc ccgacttcgt caaaggcctc gcggatttgc
      181 cccaaaggaa accggtgcgt gatgaactcc gccgaggtta gccgtccctc ccgaatccac
      241 tggcagagct gcggctggca ttctttttcg aagcggcgcg tgggccactg atggacgagc
      301 aggttgaaat tgaaatccgc gcgcgctttc tcgatgtgca gcaccggctc ggtgagcacg
      361 ccatagatgc agtgggagcc gccccggcgc agcagcggca ggccttcgag caaaatcttc
      421 gagctgccca cggcgtccac cacggcgtcc agcttgcgcg gcggcgctgc ctgccccggc
      481 gcaaaggccg cattcgcgcc taaggccagc gctttttgcc gcttctcctc atgccggtcc
      541 acgatgccga tccagcccag cccgaacaga cgccccagtt tcacaaaact cagccccacc
      601 ggccccgcgc catagatcag cacatcatcg cccggctgaa gatgaaagtc ccggaacgcg
      661 cccagcacct ccctccatgt gcagagcagc accgcctctt ccggagggat gtccgcatcc
      721 accctcgtct ggatttcatg cacctccacc cagccatgcg cctcatccgc cacgccatcg
      781 gccaccatcg tctcgtggtc cgtggccagg gtgaactcgc caaaaccgcc ccagcccgag
      841 tccacgccct ccatttgcag atcaaacacc agcccgccca ccacgcgatc cccgaccgca
      901 aagctgcgcg cctttccccc cgcctccacc accacaccca cgctctcatg cccgagcgcg
      961 aagggaaagc ggtcctccat cccagggaag cgcccgtgca gcagctcgct gtccgtgcgg
     1021 ttgcagatgc aggcccgctc cgtgcgcacc agcgcctggt aggggccggg cacaggcacg
     1081 ggtgtgtcga ggatttcgag atggcccgga tgaatggcga tgacggattt catgcgccgc
     1141 atcagagacg atctttgaat ggcgcgcaac aattctgcat ttggaaacgt ttctggattt
     1201 ggaataatga aaagcactct cgaagaccac gccgccatcc ggctcgacga caactccgcc
     1261 gcgcccggca cggagcgtac gctcgccatt ctggagctgc tcggacggca ccgcctcggc
     1321 ctgaacctca ccgagatcgc ccgcgagctg gacctgcccg tcaactccgt cttccgcatc
     1381 accggcgtgc tgcttgcgcg cggctacctc cagcggcgcg aggatgacag gcgctttgtg
     1441 ctgacgaaca aactcttcga tctcgcccgc ccccaggtgc gggagaaaag cctcgtcgtc
     1501 tgcgccctcg aagcgctcaa ggaactacgc gaccgcaccg gcgagaccgt gcagctcctc
     1561 acagccgtga atcacaagat gaccgtgctg gagcagtgcg tctcctccca ggccatcaaa
     1621 gtcagcagca ccgtcggcct gcgcgtgccc atgtattcct gcgcgcctgg caaggccgtg
     1681 ctcgcctttc tgccgcccgc cgagctggag gcgttcttcg ccgcggttga gctgaaacaa
     1741 ttcacaccca acactctcgc cactcgcgag tctctccagg ccgaactcgc acgctcccgc
     1801 aaacgcggct acgccaccga ccttgctgaa ggcctcgaag gcatccactg cgccgccgcg
     1861 cccgtgctcg acgatcatca ttaccccgtc gccgccatca ctgtcatggc cccggccttc
     1921 cggctgaaaa aagatcggtt tgccgaactc ggccgcgcct gcgtcgaagc cgccgaaacc
     1981 atccgcagga ggttgctggc atgagagccg aacatcaggc agttggagtt caagctttag
     2041 cttgttctgg aaaaccctcg cagcgaagca cgctcccgtg tcgcctaaag gcttcactcc
     2101 aactttttgt gctctctgcg ctcctttgca ccccatcccc cgccgccacg cccgcccaga
     2161 tcgagttctt cgaggcccgc atccgtcccg tgctcgcgca ggagtgctac gagtgccaca
     2221 gcgagtccgg caagcgcaaa ggcggtcttc tgctcgactc ccgccccggc tggcaggagg
     2281 gcggcgatag cggcccggcc attgtccctg gtgatgccgc ctcctcgctc ctcatccaaa
     2341 gcatccgcca cgcgcattcg gacctccaga tgcccaaaaa cggcgccaag ctcgatgtca
     2401 acatcatcgc cgatttcgaa cgctggatca ctgaaggcgc gcccgacccg cgcgacaccg
     2461 cccccacacc cgagcagcta cgcgccgaca ccgattggaa cgccgtgctc cagcgccgca
     2521 agcagtggtg gtgcttccag ccgctgcaaa cccagccccc cgccaaaccc ctctcccccg
     2581 aacaactcgc cgccgagata gaccgccagc tcgacgaaaa aagccacgct gcgggcctca
     2641 cccccgccca acccgcagac aatgccaccc tccatcgccg cctcagctac atgctcactg
     2701 gattgccgcc tgatccgtca catccgcctg atccgaccgc tcttttgacc agccaagcct
     2761 gcgccgaaaa atggtcccgc cactggctcg actggctgcg ctacgccgag acccacggca
     2821 gcgagggcga cacccccatc ccttatgcct ggcgctatcg cgactatgtg atccgcgcct
     2881 ttgcagatga cgtgccgtat ttccaaatgg tgcgcgaagc cgtcgccggt gatcttttgc
     2941 caaacccgcg catcaaggac ggcgtcaacg aaagcgccct cggcattggc cagcttcgca
     3001 tggtgcttca cggcttctca cccactgatt cgctcgatga actcgtcacc tggaccgaca
     3061 accagattga gaccgtcagc aaagccttcc aagcgctcac cgtctcctgc gcgcgctgcc
     3121 acgaccacaa attcgacgcc atcagccagg ccgactttta cgcgctctac ggcatcctca
     3181 ccagcacccg gcccgccatc attgacgtga atgcccccgg catcggggag gccgagcgcg
     3241 ccgacctcca gcacttgaaa aagcaaatcc agtccgccgt cgcccgcgct tggatgaaag
     3301 ccctgccgga aaaaaccgaa ggcggcgagt cgcccatcac tttgcccgcc actacgcatc
     3361 actgggatct gcgcaaagaa aaaaactggt tcaccgatgg caacggcctt cgccagggag
     3421 ccaccgcgcc gggcgagttc agcattgcgc tcgaaggcgg ccgtgtgatt gcgaacctcc
     3481 atcccggagg ccttttcacc gacctcatct ccaccgccga ccgcgccgta ctcatgtcgc
     3541 cacgcttccg ctgtgaaggc ggcacgcttt ggttccgcgt tgccggtggc ggcggcgcgg
     3601 tggcgaaata cgtcgtccaa aactaccccc gcaccggcac catccataaa gcgcgcgaac
     3661 tcaagaccga cagggacgcc gtgctcggct ggcacaagct cgatctggag tactggaagg
     3721 gtgacgacat ccacatcgag ctcgctaccg ctgccgaccg gcccgcacag gccgagttcg
     3781 acgcccgctc ctggttcggc atcaccgagg ccttcatcac gcactcaagc gacaacccgc
     3841 gcggccccgg cattccctcc aaaccgggac aagacgccgt gcgcgcctgg ctcgccggca
     3901 ccctcaccga cggacaggcc gaagccctca accgcgccct gcaatcgggt cagctgccca
     3961 accaactctc cgccatcccc gaagccgccg ctctggtcga aaaataccgc ctcctcgaag
     4021 ccaagctgcc ccgtcccacc cgtgcccccg gcgttctcga aggcgacgcg cgcgatgccg
     4081 cgctttttgt tcgcggcaat cacaagcagc ctgccgacct tgtgccacgc cgctttctcg
     4141 atggcatcaa ccccgtgcct tttgagacca agcaaagcgg ccgcctcgag ctcgccgccc
     4201 atctcaccga tccgcaaaat ccgctcaccg cccgcgtcat cgtcaaccgc ctctggcacc
     4261 atgtcttcgg ccgcggcctc gtcgccacca cggacaactt cggccgcctc ggccagacgc
     4321 ccacgcatcc cgagctgctc gatttcctcg ccgcccagtt catcgccgat ggcggcagca
     4381 tgaggcgctt catccacgcc ctcgtcagca cccgcgcctt cgcccgcagc gccagcgcct
     4441 ctgcagccga tctcgcccgc gatcccgaca acctccacct cgcccgctgg accgtgcgac
     4501 gccttgaagc cgaggccatt cgcgacagca tcctgcacct ttcaggcaag cttgatgcca
     4561 ctcccttcgg ccagcccgtg cccggcaccg cgccgcgccg cagcgtctat gtgcaggtca
     4621 tccgcaacca gctcgatccc ttcctcactg ccttcgacat gcctgtgccc tccgcaccgc
     4681 gcggcgcgcg agatgtgacc aacgtgcccg cgcaatcgct cgccctgctg aacgatcccg
     4741 ccattcaaac ctgggccgcc gactgggcag cgcgcaccga gacccagctt gcgcccgagc
     4801 agcgcgtccg cctcatgttc cagcaggcac tcgctcgtga acccgagcca aacgaactcc
     4861 aggccagtct ccgttttgtg gaaagccacc tcaccgaagc acgcgcccgc caggaccgca
     4921 tcatcgcgtt gcgccgccaa gtggaggtcc tgcttgcatc ggtccgatcc gtcggctccg
     4981 tcagatcagc cccatccaaa gtcctcgcgc ccctcgccga gtggaccttt gaaagcgacc
     5041 tcaccgacac ccaaggccgc ctgcctctga ccctctccgg tgcggcccgc ctcgaaaacg
     5101 gcgcactcgt cctggatggc agctccatgg cccagaccgg ctcgctgccc aaaaccctca
     5161 ccgccaaaac cctcgaagcc tgggttcaac tcgacaacct cacccagcgc ggcggcggag
     5221 tcattaccgt gcagggcaag gatggcgtgg tgtttgactc catcgtcttc gccgaaaaac
     5281 agcccggcca ctgggtcgcc ggcagcgacc acttcatgcg cagcgaaccc ttcaactgcc
     5341 ccgccgaaac cgaagccgcc aaccgcatcg tccacctcgc cgtcgtctat gaagccgacg
     5401 gcaccgtgcg cggctatcgc gatggcgaac cttatggccg cgcctatcgc aaagcccctg
     5461 gcgccgtctt cgaagccgaa agcagccaga ttctcctcgg ttgccgccac ggcaagccca
     5521 gcggcaaccg cggcctcgcc gcccgcatcc accgcgcccg cctctacgac cgcgccctca
     5581 ccgaggaaga aatcgcccaa accgcccgcc tcgaaaacct ccctgtcacc gaccacgctc
     5641 tcctctccgc cctgccaccc gagcaacgcg cccaagtcca gaaactccgt gccgagctgc
     5701 aaaacctcga agcccaagcc cccaacgaaa gcacccccga agccaccgcc tggcaaagcc
     5761 tcgcgctgtc gctcttgaac ctgaaggaat tgatttacct gcgatgaagc cagagccttt
     5821 tcaatcgccc tcgccgtggt atgctcgcca tcatgcagca gcagccacgg cttcagaaag
     5881 tcatgaccac cttcgcctcc caccaggaaa gggagaaagc ggagcgggcc gaatggcgtg
     5941 ccatgacacc cgttcagcgc ctgcaagccg tcgaaaccat gcgccagatc aaccacccgg
     6001 cgtatgatcc cgctacctcc agacttcagc gattttatac ggttgcttaa ccgccaccgc
     6061 gtccgctatg tggtggtggg aggttacgcc gtggccttcc acggctatcc cagatacaca
     6121 ggagacatcg acttctttgt cgagaagtcg gcccgcaact ccgtggctct gcaaaaggtt
     6181 ttcagagact ttggattcgt cccaccaccc gaagcctcgt tgtttctgga agacaaacgc
     6241 attgtgcgca tcggcttccc tccgatgcga gtcgaggtct ccaatcacat tgacggtgtc
     6301 acgtttgcgg aatgccatga aaatcgcatc caagcgcgcg tggcgggaat tcgtgttcca
     6361 ttcattgatc tggaacgcct tcgcatcaac aagcaagcct gcgggcggcc aaaggatatg
     6421 gaagaccttc ggcagctttt tggcgaagca ccacaggact gatccatcgc tccaccaacc
     6481 acgctatttt acgatgaacc gccgccacct cctcgcccgc gcctccaatg gctttggtct
     6541 cgccgcgctc gccggactca tggccgagaa cgcgcctgcc gccgtcgtca cgcaccgctc
     6601 caagcgtttc atcccccgcg cgcgcaacgt catcttttgc tacatgagcg gcggagtctc
     6661 gcacattgac agctttgatc cgaagccgct gctggggaaa tacgcgggca agccgccgcc
     6721 cgtgcccacc gcgcgcacgc agttcaacaa caacggcgtc gtgcagccct cgcactgggc
     6781 cttccatcgt cacggccagt cggggctgcc cgtgagcgaa ctctttccgc acatggccag
     6841 cgtggccgac gaactctgcg tcatccgcag catgaccgcc aagttcagcg agcacgcgca
     6901 gggaaacttc ttcatgcaca ccggcttccc ctttctcggt tacccgagcg cgggcgcgtg
     6961 gaccagctat ggcctcggca ccgagtcgcg cgacctcccc ggttacgtcg tcctgcaaag
     7021 cggcggcgcg ctggctccgc atggcggcgt cggcttgttc ggcaacggat tcctgcccgc
     7081 gcagcatcag gcctccatca tccgcgtgga cgagaccgaa cctgtgcgca acatccgccc
     7141 acgcgaagcc gccgtgcttc agcgacgccg tctcgatttc atcggcgaac tcgatcaagg
     7201 cttcctcggc gaactcggcg cgcatcgcga ggtcgaggct gccatccaaa actacgagat
     7261 ggcctggcgc atgcagtctg ccgtgcctga tctctgcgac ctctccggcg aaaccgaggc
     7321 cactaagaag ctctacggca tggacggtcc cgacagcacc cgcaaagcct atggcatgca
     7381 gtgcctgctc gcccgccggc tcgtcgaacg cggcgtgcgt ttcattgagc tgagctgcct
     7441 gccgcagcag ctcggcgccg ggcaggcgcc caacccctgg gaccagcacg gacaaatcaa
     7501 agaaggccac ggcaacatgg cctggcaggt ggaccagccc atcgcgggtc tcatcaagga
     7561 cctcaaatcc cgcggcctgc tggacagcac gctcgtggtc tgggcgggtg agtttggccg
     7621 caccttcttt gcccagggca gcgacggacg cgaccacaat ccctacggct tcagcctctg
     7681 gatggccggc ggcggcgtgc gcggcggcat gaccctcggc gagaccgacg acctcggcta
     7741 ccacgccgtg gagaaggtgc agaccgtgta tgacctgtgg gccaccgtgt tgtaccttct
     7801 cggcgtggac cacgagcagc tcacctatct cttcagcggc cgcaacttcc gcctcaccga
     7861 tgttcatggg cacgtgatgc gcgaaatatt aatctgatca tcggccatgc cattgctgtt
     7921 ctgtcggcat gtcttttcca gccttcaagc tttagatttt ggcaaaaccg agcatgagtg
     7981 ctgatacacc atcatctcag gccatgaatg acacatcctc cgatcaggtg cctgcccatc
     8041 atctctgggt cgagctttcc acgcgcacta cggctgagac tgctcatcaa cattcagggg
     8101 atgaggaggc ggcaatgaga agcgtgcatt ttttgtccgg caaaacgagg gagctgatgg
     8161 ccgggcatcc ggcggccatg gaattcaatc gcctggcgga gcggctgctg aatgagatgc
     8221 tgcggccttg catggcgagc tggcatggct ggatgacacc ggacccgaat ctgcgggaca
     8281 aggacgggca gccggtggcg aaattccgcg atccgcaggc gcggcagttg ttccgagcgg
     8341 agttgggtga tctgcacggc agactgctgg catggcagcg agtctttgat ttgctcagga
     8401 ggggcctggc gagggatgag cagggggtgt cccttgatga ttggtcccgc cccacgaacc
     8461 agagactggc gctcttggca aatcccgatg acaagccggc gaaatccggt ccgaacctcg
     8521 gcgcggtcct gccgttgggc atcggtggcc aggtggaggt ggctggcgat gtgaaaatgt
     8581 ctgaaatcgc gaaagcggaa atcgcggaga ttcatcaccg gcgcgcggtt ttgaaactgg
     8641 tacggccaga ggataaaaaa tcggcggtat ggaatgtggc gggcctggcg ctctctggcg
     8701 gcggcatccg ctcggcgact ttttgcctcg gcatcacgca gatgctggca agacacggct
     8761 ttttgcatcg cttcgactac ttgtccacgg tctccggcgg cggttatttg ggcgcgtttt
     8821 tgagcacggc tctcggcacg cggcaggagg gggacaaaag caggacgact cctgaaagcg
     8881 cggctgacaa actgaaggat gtgtttgaga ggacggaggg catcgagtcc gggttggtgc
     8941 ggcatctgcg caacaacagc aaatacctgc tcaatggcgg tctgggtgcc aagctggaga
     9001 tcacggggtt gctgatcagc ggggtgttgt ggaatctgct catgctgctg ccactgccgc
     9061 tgatcctggc atgggtggca catgtcgggc aggaatggat ctggggggcg cagatcgttt
     9121 ccggtagttt caaagaaccg gactgggcaa agggctgtgc tgcggatttg gcgattcttt
     9181 ttgggggtgt gctggccttg ggctggttca ctcagccgct ggtgaggatg ctttcccacg
     9241 ggcgcgatcc gaaggacaag tccatgctgg caagacgcgc gctggaggtg gcgctgccat
     9301 gggttggact tgcatttgct ttcatggtct ttgtgaactg tgtgcccaca ctgttttatg
     9361 ggtatgaaat tctgcgccag gagtataagg cgctgggcct gcaatggctc aaagggactc
     9421 tctctgcgga tgttctgggc gtttccgcgg gtggtgcttt ctccgcggct ctggctctgg
     9481 tggtggcaaa gctggccccg catcgcgaga ggctgcgcag gctggcggtg cggctgctgg
     9541 tcttgagcgg gcctctgctg ctgcttgcca tttttctggc ggtgggaaat cgcatggggc
     9601 ttggccgaac ggttgtggag ccggaggtgg tgccagtttt tgtgaccatg atcccgacgg
     9661 ttctggctca gtattcccag gaatacctgg ccgacctttc cacaaaatgg tcgcccgcgt
     9721 gggttttgac cgtggcgatt gggatcgtgc tctggggctg gttgggggtg aacatcaaca
     9781 ctttggctcc gcatatttat taccggaacc ggctttgcga ttgctacatg gtccggcgca
     9841 tcgaaccgga tgcggagaag ccggggttat ggacccgcct cgtgcgccgg ctttccggtg
     9901 agaaggtgca tgggcgcacg gagacactgc agcgagtgcg cttttccagt ctgaattcgg
     9961 acctcgccgc gccttatcat ttagtcaaca tgactctcaa cgctccctcc agcacgaaca
    10021 agaacttgcg cgggcgcggc agtgattttt tcgtcgccag ccggagatac tgcggctcgc
    10081 cgctcacggg ttacaaaaag acggatgaga tcacctccgt ggacccgcat ttcgacctcg
    10141 gcacggccat ggcggtctcc ggcgcggcgg cctccacgag catgggctgg aaaagccttc
    10201 cgaactttcg ctttttgatg acgctcttta atgtgcgtct cggctactgg ctgcgttggg
    10261 gcggcaagcc gggtctctgg caccggctgg cgaacacacc gggtccggtg tatttgttcc
    10321 gggagatgtt tgcggtgatg gatgagcgct gccgttactt gaatctctcc gatggcggtc
    10381 acatcgagaa tctcggcgtc tatgagctgc tgcgccggca ggcgaagttc attgtctgtg
    10441 tggacggagg acaggagccc ggcatggaat gctcggatct cacccgcctc cagcgttatg
    10501 cggagataga cctgggcatc cggctgcatt ttgatctgca tgatctctcc ccgatgccgg
    10561 acggctgctc ccgcgcttac gggctgctgg tgaaggtgga ctatgaaccc ggcgagagcc
    10621 ggcccgctgc cgaggccaag gtggggtgga tgctatatct gaagctggca atgacgggcg
    10681 cggagcccgc gcatgtgctg gattacaagc gcgatcatcc cgacttcccg caccagacca
    10741 cgggggatca gattttcgac gaggctcagt ttgaggctta ccgccaactc ggggaatgcg
    10801 cgatgcggag ttttttccac gacaagcttt tcgacgccgg cgctgacacc agtttggatg
    10861 actggttcca tcatctggcc tgccgtcttt tgccggacaa tgacgacgct tatggttaaa
    10921 cgcatcatgt cacgaataaa gagcggctgt tgagacatca acagccgcga ttatgaaacg
    10981 gagcggtgtg cctggggtgc gggtccgcgg ttattccgcg gcttcatttt tgctagccgc
    11041 accctggcgc tgccccgctt cacggtcgag cacaagcaag ctggcggggt cgttgcctgc
    11101 gagcttgagg cggtcaagca tgtcgcgcac cgttttttcc tcctccatct gttcgttgag
    11161 gaaccaaagc agcaggtttt tcgcggcatg gtctttgacc tgctccgcca cgtcatagag
    11221 atcattgatc tgcttggtga cgagttgctc ctgcttgagg ctctgctcga aaacatcaag
    11281 aggagatttg aatttgtgtt tgggtttgga gatgggctcc aattccacaa cggcgtcgcg
    11341 gtccaccaca tactggtaga acttcagcgc gtgcatcgtc tcctcgcggc tttgattgaa
    11401 catccagctc gcgaagccac tgtagggcgt ctgctcgaac caggccgaca tggcgaggta
    11461 ggtgtagctg gaggccattt cgttgttgat ctgctcgttg agcagcttgg tgagcttggg
    11521 atcgagggtc atggtgtgag ggcagggttc agtgccgaat acaacctgat aaacgctcca
    11581 aaggcaactc cgggatgctt tccaatgctg atcctgctct cgcctcaaac agccaagact
    11641 tccagcggca tcgtccattt agaacaccgg ggccgtggcg tattatccga gtccgaactc
    11701 aacccccacg cgcccatgtt caccttcacc gacaaaggtt ccgtcaccac ctgcgatggc
    11761 gtcacgcgcc gcgatttcct ttccgccggc actttgagcg ccatcgggct cacgctgccg
    11821 agttttgcca aattgaaggc ggaaggcaaa gtggacccga aaaaaagcaa caaggcctgc
    11881 atcatgatct tcaacctcgg tgcgccgagc cagcaggacc tgtgggacat gaagccggac
    11941 gcgccgagcg agattcgcgg ccccttcaag ccgatccgca cgaagagcaa tgccttcgag
    12001 atttccgaaa tcctgccgat gcatgcgaag atcgcggaca agttcagcct cgtgcgttcc
    12061 tgctaccaca ccgccgccgc ggtgcatgac acggggcacc agatgatgca gacaggccgt
    12121 cttttcaccg gaggcgtcat cacgccgcat gtcggcagtg cgttggagtt cctgcaaggc
    12181 cgccgcagcg atctgcccgc gcacatcatc cttccggaga cgatgggccc caccggtggc
    12241 aacatgccgc acggccagga cgcgggcttc ctcggcaagg cgtatgatcc tttcgtcctg
    12301 aacgcgaatc cgtcggagaa ggacttcaag gtgcccgatc tgctgccgcc gaaggaaatc
    12361 agcgaggtgc gtctcgaacg tcgcaagcag ctccgcgagc tggtggacag cagcgtgcgc
    12421 aatttcgaat acagcgagca ggcgaagctc atggacagca atttcgagtc cgcctaccgc
    12481 atcatgacca gcacgcaggc ccgcgaggcc ttcgatttga cgaaggagcc gcaaaaagtc
    12541 cgcgagcgct acggcatgaa ccgctttggc cagagctgcc tgctcgcgcg tcgtctcgtc
    12601 gagcgtggcg tgcgtttcgt caccgtgaac accttcatca ccgtgtttgg cgagatcacc
    12661 tgggacatcc acggctccaa acccttcacc agcatcgaag gcatgcgtga catcgtcgct
    12721 ccgatgtatg accagggcta cagcgccctg attgaggacc ttctccaacg cggcatgctc
    12781 gacgacacca tggtcacctg cctcgccgaa ttcggccgca cgccgaagat caatcccgcc
    12841 ggcggtcgcg accactggcc gaactgctgg accgtgaact ttgccggcgg cggcgtgaaa
    12901 ggcggcgaag tcatcggcaa atccgacgac atcggcggct atcccaccga gcgccccgtc
    12961 gccccgaaag aaatcgtcgc cacgatctac caggccatgg gcatcgacct ccacaccgaa
    13021 cttcccggcc ctcaaggcag acctttcccc gtcgtcgatt tcggcaccca ggcgatcaag
    13081 gagctgtttg cgtgatttca atctggagcg cggacgccct cgtccgcttt cacctcttcg
    13141 tcatgaactc agtgccacaa acacgtcgaa aatgggaact cggcatcgcc gggctgctca
    13201 ttctagtcac ggcggtctgt tctggttgga tgtgcggcat tttcgggaac gtgccgccct
    13261 atccccaaga gttccatgac caagaagttc gtcggaggat cgaaacagcg aacaagcttc
    13321 aagagcagtc cattccacga ggagaggtgc attacccatg gtataactgg gagccacaac
    13381 aactcgatac gttgcgggat cgcttgagct gggaaagtgg taagataagc attcaaccgg
    13441 tcgcctatat gaacggcgtc gagcctccga aaccagggca gatctccgtg tttttgatca
    13501 atgacactga cgagcaactt caggttccgg cccaagatgg agacatctac ctcaaactag
    13561 aagtgaatct ggaggggaag tggcaacgcg ctcaatgtca tggcgacagt tggtgcggga
    13621 acagctacgg aaccagaacg ttggctgcac acagttacgt gatcatccaa ggataccagc
    13681 caacacctgg ccgacgagcc aaagtgcggt acgccatcca caatggcgcg tcggatcgcc
    13741 tgacatcggc gaagttcgat ggactttacg cccctgatga cgtggcaatg agcgcgtatg
    13801 attcgctggc tatgcgtgag gctgaactct ccaagctgcg caaggttttg ctaaggcagg
    13861 acacacccac ccttatgccg gggagctaca cgatagacat gatgaggcgt tcagcttggt
    13921 atggcatcct gcgaggcggt catgaccgtg acgcggctct caagattgca gaggaggttc
    13981 ttgcttatga cccgactcta gagcctcgga aagccgacct cggtcaactg atggactcct
    14041 ggcatgccgc attagaacat gaaactcggg gtcgagtgat gattcacgga ttcactgttt
    14101 cgaaacgtct cgttcaaaac acatcgcatc aagctcacca aaacaacggc acgccgtaag
    14161 ccgcgaactc cgagtcacac gccaccaaat gaaacccgcc cgtcaatgcc tgcgcgatga
    14221 tcatccggtc gaatggatcg cgatgatgca tgggcaggct gtcgagccgg agaatatcgg
    14281 gcag
//
LOCUS       DAOMYJ010000129        14207 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Prosthecobacter sp. isolate AMR_MDS_5629
            NODE_1434_length_14207_cov_22.077869, whole genome shotgun
            sequence.
ACCESSION   DAOMYJ010000129 DAOMYJ010000000
VERSION     DAOMYJ010000129.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867850
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prosthecobacter sp. (wastewater metagenome)
  ORGANISM  Prosthecobacter sp.
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Verrucomicrobiaceae; Prosthecobacter.
REFERENCE   1  (bases 1 to 14207)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14207)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.30
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:34:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,742
            CDSs (total)                      :: 4,684
            Genes (coding)                    :: 4,676
            CDSs (with protein)               :: 4,676
            Genes (RNA)                       :: 58
            tRNAs                             :: 55
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14207
                     /organism="Prosthecobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1434_length_14207_cov_22.077869"
                     /isolate="AMR_MDS_5629"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1965333"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..258)
                     /locus_tag="PLP58_13625"
     CDS             complement(<1..258)
                     /locus_tag="PLP58_13625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ09612.1"
                     /translation="MMKRLLLTLLLPLALRAQVLVESLPEPGVQPQVAVTAAGVVHLV
                     YLLGDPAACDVRHTTRPAQGGPWSAPVTVNSVPRSAVAAGTI"
     gene            complement(509..757)
                     /locus_tag="PLP58_13630"
     CDS             complement(509..757)
                     /locus_tag="PLP58_13630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ09613.1"
                     /translation="MKTKLTTLLTTALLGLALASCSTSGGAKPYPFDVCIVSGNKLGS
                     MGTPITKVHQGQQIKFCCEPCVEEFDETPAKFMAKLPQ"
     gene            complement(811..2946)
                     /locus_tag="PLP58_13635"
     CDS             complement(811..2946)
                     /locus_tag="PLP58_13635"
                     /inference="COORDINATES: protein motif:HMM:NF012612.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multicopper oxidase domain-containing protein"
                     /protein_id="HRJ09614.1"
                     /translation="MKRFLLPALFGPSMAALACDVCQTSRIPASSRVVEYDLHIREQT
                     VSPAGKAVRGFTINGGIPGPTLRFREGEWARIRVHNHLKRETTSTHWHGLLLPNEQDG
                     VPHVTTPPILPGKTHTFEYELRHSGTYWYHSHTHLQEQSGVYGSIVVEPRGGEPVRAD
                     REQVLVLSDWTNFNPHEVMRMLMRGSDYFGLMRRNAQSVLGAWQRGNLKDYFQREWDR
                     MMPMDVSDVGYHAFLINGQRQTLIEGRPGERVRLRIINASAASYFYLHSSAGPLTIVA
                     ADGPPVQPVQVERLFMAIAETYDVIITIPPSGQYELRATTQDGSGHASAFFGSGALKP
                     ASDPPRPNLYQMNEMLRLALEEQKDDPRASLNLPRPGSPYRVLKAVHDTTLPAQLPRR
                     KITMHLTGDMNRYIWGFDGKTIAQEPYVRIKKGEVIELELVNDTMMHHPIHLHGHFFR
                     LLMGNGARSPLKHTVDVPPMSRRIIEFEANEEKDWMFHCHILYHMMSGMARVFRYEDE
                     VGANASLRPHTTSHDSPRSLAAAPADHSLHHAAGLGEHSHDMAYVWGAASLQSHMSEG
                     LLTWMNPKNDLLLAWEVGWEGVDNPQYEIDALYQRYFNLNFQAFAGYRLTNDPDAKDR
                     GLIGFNYRLPLMTWANLSLDTEGDARLTLSKRLQLTPRLGVWGEVFYDTGTEWEWTAG
                     ADYTLTRHTSLTLSYHSDFGLGAGMLFRF"
     gene            complement(3048..3398)
                     /locus_tag="PLP58_13640"
     CDS             complement(3048..3398)
                     /locus_tag="PLP58_13640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ09615.1"
                     /translation="MTLLRSIVCLFALLAQAWPVLAMPRTAELEACAMECCAAMSLCE
                     CAEPSAPAEPANAPPVSGRKWAPQAVWVPVEVAGPLPRSPRALNEGASRWAESSFENQ
                     PQVRLAVLFCSFLN"
     gene            3573..4664
                     /locus_tag="PLP58_13645"
     CDS             3573..4664
                     /locus_tag="PLP58_13645"
                     /inference="COORDINATES: protein motif:HMM:NF014035.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HRJ09616.1"
                     /translation="MSIQAPRKQHLWVWFLWLAGFYGVWTWLVFGNDRWGDVTAHWPI
                     ALAMAAGSYVAGSTPMGGGTVGFPILVLLFGLPASLGRDFSFAVQSIGMTSASIFILS
                     RRQPLAWSMLKGSVFGAVLGLPAGLFFIAPYVPELWIKLVFAVIWCSFGVLHLYRIGE
                     IASHDGMTEFDERWDFKVGTVLGIFSAATVASVTGVGIDMVLYATLVLLCRADLKIAI
                     PTSVIIMAFTSLLGVLVKNLTTGMQPGVYENWLAAAPVVALGAPLGVFMVNLIGRKPT
                     LIFVAVLCVGQFVWTCHTERAALGIPGFILALAAVGGCLLVFERLRVWGGVLVGEAKA
                     KHAAGSSAADGRQEDATHSKEAAATCLQG"
     gene            complement(4847..5287)
                     /locus_tag="PLP58_13650"
     CDS             complement(4847..5287)
                     /locus_tag="PLP58_13650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ09617.1"
                     /translation="MKTKLSLLVTLITSLAFSHEGVELGPNGGRIVEFSNNETLHGEV
                     TVKGGTIHIAVLGKDMKPVAMDKQSLTATTGDRAKPVKLEVTKDAKGFVLPIVKPGEW
                     LILRYKDNADAKAVTARFEYNTATCDGCQAPEWLCKCKPDAEKK"
     gene            complement(5284..8427)
                     /locus_tag="PLP58_13655"
     CDS             complement(5284..8427)
                     /locus_tag="PLP58_13655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009963327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HRJ09618.1"
                     /translation="MLNSLIRFSLHHRPLILMLALVTLVFGYQALTQLPVEVLPDMTK
                     PTVTILTEAPGLAPEEVEALVTQRLEAAVQGVGGLDRLRSNSDVGLSLIFAEFGWGMD
                     IYRARQLVQERMQGMLSTLPEGVKPGLTPVSSLMGEILLVGLRCSKKPGEPGYISPMD
                     LRTLADWTLRRRLQSISGIAEVLTIGGGVKQIQVQPDPYRLAAFGITLAEVEEAVGRA
                     AGNATSGYLQAGPQEIMVRNLGMTTSLDDLAKTVIKTVGSRPVLISDVAEVRHAVQTM
                     RGDASVNGNMGVILSIDKAPGFDTLTLTQQIEAALEEFRPTLPEGVTVEVLFRQGDFI
                     EHAIGNLKEAIRDGAIMVTIILFLFLLSLRTTAITLMAMPLSFAVTILTFKAFGVSVN
                     SMTLGGLAVAIGMVVDDAIVDVENVFRRLRESGAGTPARKEGAHRGQECPHHLETIAS
                     ASSEVRNSILYATVLIILVFLPLLGLEGIEGRLFTPIAIATITSMAASFVVSLTVIPV
                     LCSLLLKAGGKAHDDGFVIRAMKWIVQRTFLKVALGAPMLVLAFAGVLLIAALMLYPM
                     MGKDFLPAFNEGSATISLVSAPGTSLAQSNEIGEVGVRLLQSIKEVKSVGRRAGRAER
                     DDHVMPVSVNEFDVEFHESGRPREVVFAEIRRLLSAIPGTFVNVGQPIGHRLSHMLSG
                     VSARIAVKIFGPDLDVLREKGAQVRDLGQSIPGLTDVNLEAQVPIPQIKIEVNRERAL
                     AYDLQPGDINAQVSTLLGGKVMAELREGQRTMDLALRLPESWRDSPEKIGNLLIETHS
                     GQLIPLNLVADIREGKGPNVINRENTQRRIVIGANTSERDLESLVRQWEKLVREKVKL
                     PEGCFLRFEGEFQAQQAATKRIAFYFALVLLAITILLFGYFRSLSLALQVMLNIPLAL
                     MGGLALTWLLLDNISIATLVGFIAVGGVAARNGIMMISHYLHLMKHEGETFGRPMIVR
                     GTLERLVPVLMTALAAGIALIPLLLAAHEPGKEILHPVAVVIVGGLVSSTLLDLMVTP
                     AVFFLFGKPPTQTPTHP"
     gene            complement(8421..9521)
                     /locus_tag="PLP58_13660"
     CDS             complement(8421..9521)
                     /locus_tag="PLP58_13660"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HRJ09619.1"
                     /translation="MKPIFCLLLLVTGAFAATPDIILDDVAVSNMNLEFAEAEETVFE
                     ETVFALGSIEVLPGKRGVVSSRIPGRAFSVLVIPHQEVDAGAEVAWVESRQPGDPPPV
                     VKLEAPIAGFVSKVNLAQGQPVSPDDALIEILDLSTVEAVAHVPQHFAAKLKLDQKAH
                     IRLQALPDKVFEAKLVHIAVEADVATGTIEAAFHVTNPDKLLRPGMKAEFSIVVSERE
                     NVMSIPRGAVQGDPAQRFVYVKDYELKNAFVKALVVLGAQNDRFVEVVSGLLPGDEVV
                     TRGAYALAFAGKGSVSLKEAMDAAHGHPHNEDGTEMTKEQQAAEHGDHDHDHEGAHNE
                     WSTLTTFFAATSGLLLLLLIVTAFKRREVVAC"
     gene            complement(9528..9878)
                     /locus_tag="PLP58_13665"
     CDS             complement(9528..9878)
                     /locus_tag="PLP58_13665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009963329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3147 family protein"
                     /protein_id="HRJ09620.1"
                     /translation="MTYYLLKLFLSAGVIVAVTEIAKRNNAAASIIHSLPLTSLLAFI
                     WLYSETKDSALIGRHAFGTFWFVLPTLPMFLLMPWLVKKLGGFWPALGAGIVLTVILY
                     FLTMALLKKAGVEL"
     gene            complement(9900..11105)
                     /locus_tag="PLP58_13670"
     CDS             complement(9900..11105)
                     /locus_tag="PLP58_13670"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HRJ09621.1"
                     /translation="MKPPSLFLLAWACASPSLALTLSLDEVASRARTHHPALKAARLA
                     VEEARGRQLGAGRLANPSLGYDFQNQSNLSPQTGVVSLDQSFPLTRRLSLEKKLTSQV
                     VAAAELEVRDAERRLIAEAKSQAVRLLTLSRQRQLRQQQMELAQKLADFASDRAEAGE
                     VSPLDARQVQLDAQKLRIESRLLEAASVAVLGQLKPMLGLRSEEPLTLTGGLPALELP
                     PASSWQQRPDYRLAQTKAEAAQTGQQLARARRIPDVTAGIFGAHEMQDVTSAQRESTG
                     FIGFRFSIPLPLWNRNQGEITETAASAERARLEAEALAAQIHGEAGTARREMEANASI
                     VREMRDTLVPLAVQHSAAMEKAYENGQADLLSVLRSRDQRLQLESAALDAERDFHLAR
                     IRYEAATSP"
     gene            complement(11213..11629)
                     /locus_tag="PLP58_13675"
     CDS             complement(11213..11629)
                     /locus_tag="PLP58_13675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ09622.1"
                     /translation="MLTATLAVAWAQTFGIHHGWLCDCGGVERITLVDHCHGPHSHDC
                     HEDEDHDVPHHHEDGEDEDTHRHTAVVDDLVAKHHEAYSAQAPVLLALASVAWEPGLF
                     RLNPACRRREEPPDKGRRDGPREWPQILTRTIVLRV"
     gene            complement(11739..13550)
                     /locus_tag="PLP58_13680"
     CDS             complement(11739..13550)
                     /locus_tag="PLP58_13680"
                     /inference="COORDINATES: protein motif:HMM:NF024240.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HRJ09623.1"
                     /translation="MRPLFTALILALPVSAAELTVDVCIYGATPGGIAAAVAAARSGS
                     TVLLLEPTARIGGLLASGLSHTDYHSRESLTGSFLEFSKRVKAHYERTYGADSQQVKD
                     SHEGVFGEPRVNLLVLEQMLAEQERVTLRKGAALKSVQVARAVISQAEFAQGAETFMV
                     RAAVFVDGSYEGDLMAAAGVRYHVGREGRDVYGESLAPEKSDKELQAYNFRFCATDDP
                     ANRAPIQAPKGYRREDFLDVLPILESGKIQSVFGYPSGCVVKAQPPALPNRKYDINDV
                     SRGLIRLSMPGANLAWPEGDAAARQAIFDEHLRYNAGLLFFLANDEAVPARFREPASQ
                     WGWCKDEFTETGHLPPQLYVREARRMTGRHVYVQQDSEHAPGDARAKLFKDAIAMGDY
                     GNNCHGTSHKGPLIGGSHGGEFYNPVPPYQVPYGALVPNELENLLVPVAVSSSHVGFC
                     ALRLEPIWMSLGQAAGHAAAMAAATKLAVDAVPVGELQKRLHKDGSATLYVSDVPPGH
                     PHFEAVQWWGTAGGLHGLNPMPEKPGQRGKNLHGQYFEANPGHALEPLKPLDRDTHDR
                     WLILANELDAYSSQLEKAPTRIDFILAARSAAEKAAR"
     gene            complement(13618..>14207)
                     /locus_tag="PLP58_13685"
     CDS             complement(13618..>14207)
                     /locus_tag="PLP58_13685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="peptidoglycan-binding domain-containing protein"
                     /protein_id="HRJ09624.1"
                     /translation="APAAPPAMTPAAAPAGLPADIANVVATSPPLIVQEKPPEPEVIT
                     PDKFVGPVIPPQAPVTEPAAGYWTVEQMYPTPPYSGYSANGKKHLLYRAQGLLKEKGL
                     YTSTLDGKEGKNTHNAILLFQVRSGLAANGFLDVPTLIAMGLAGESDKPDWTGGSSVA
                     SGGYRKRSTKSAEEPNFFQRATKSVGKLFGGGDKK"
BASE COUNT         2411 a         4385 c         4806 g         2605 t
ORIGIN      
        1 gatggtgccc gcggcgacgg cggagcgcgg cacgctgttc acggtgacgg gcgcggacca
       61 ggggccgccc tgcgcagggc gcgtggtgtg gcggacatcg caggccgcgg ggtcgcccag
      121 gagatagacc agatgcacca cgcctgccgc cgtcaccgcc acctggggct ggacgcccgg
      181 ctccggcagc gattcgacga ggacctgggc gcgcagggca agcgggagca gcagggtaag
      241 aagcaggcgt ttcatcatgg cggcatggag ggttgcatga gcacgtctcc ccatgccgga
      301 cctttaaacc cgaacgccgc gattgatcca ggccatattc cggaaatccc ggccccaagc
      361 ggctcgctgg ctggagattc ggtgttttca aatacagtcc cccagcacat cgaaccgcct
      421 gggactcagg ctttgcagaa tctgtctctg actgaatcac ggcgttcggg tccaaggatc
      481 ccgcacggga agacggaggg ggccgccatt actgcggcag cttggccatg aacttggccg
      541 gggtttcatc gaactcctcc acgcagggtt cgcagcagaa tttgatctgc tggccctggt
      601 ggaccttggt gatgggcgtg cccatgctgc caagcttgtt gccgctgacg atgcagacgt
      661 caaacggata cggcttggcg ccgccggagg tggagcagga ggccagcgcg aggccgagca
      721 gcgcggtggt gaggagggtg gtcagtttgg ttttcatgat gttgggtctg tgggtttggg
      781 gtttgttttt ttcaatgacg gcgtgcgcca tcaaaagcgg aagagcatgc cggcgccaag
      841 gccgaagtcg ctgtgatagg agagggtgag ggaggtgtgg cgggtgagcg tgtagtcggc
      901 gcctgccgtc cactcccact cggtgccggt gtcataaaac acctcccccc agacgccgag
      961 gcgcggggtg agctggaggc gcttcgagag ggtgaggcgc gcgtcgccct cggtgtcgag
     1021 gctgagattg gcccaggtca tgagcggcag gcggtagttg aagccgatga ggccgcgatc
     1081 cttcgcatcg gggtcgttgg tgaggcggta gccggcaaaa gcctggaagt tgaggttgaa
     1141 gtagcgctgg tagagcgcgt cgatctcata ctggggattg tccacgccct cccagcccac
     1201 ctcccaggcg agcaggaggt cgtttttcgg attcatccag gtgagcagcc cctcgctcat
     1261 gtgggactgg agggaggccg cgccccagac gtaggccatg tcgtgcgagt gctcgccgag
     1321 gccggcggcg tgatgcaggc tgtggtctgc aggcgcagcc gccagactgc ggggtgaatc
     1381 gtggctggtt gtgtgaggcc gcaaactggc gtttgcgcct acttcatcct catagcggaa
     1441 gacgcgggcc atgccggaca tcatgtggta gaggatgtgg cagtggaaca tccagtcctt
     1501 ctcctcgttc gcctcgaact cgatgatgcg ccggctcatg ggcggcacgt ccacggtgtg
     1561 cttcagcggt gagcgcgcgc cattgcccat gagcaggcgg aagaaatggc cgtgcagatg
     1621 gatggggtgg tgcatcatcg tgtcgttgac gagctccagc tcgatgacct cgcccttttt
     1681 gatcctcaca tagggctcct gcgcgatggt tttgccgtcg aagccccaga tgtagcggtt
     1741 catgtcgccg gtgaggtgca tcgtgatctt ccggcgcggc agctgcgcgg gaagggtggt
     1801 gtcatgcacg gccttcagca cccggtaggg cgatccgggc cggggcagat tcagggaggc
     1861 gcgcggatcg tccttctgct cctccagcgc cagccgcagc atctcgttca tctggtagag
     1921 gttgggccgc ggcgggtccg aggcgggctt cagtgcgccc gacccgaaaa acgccgaggc
     1981 atggccgctg ccatcctgcg tggtggcgcg cagctcgtac tggccgctgg gcgggatggt
     2041 tatgatgaca tcgtaggtct ccgcaatggc catgaagagg cgctccacct gcaccggctg
     2101 caccggcggg ccgtccgccg ccacgatggt caaaggcccg gcggaggaat ggaggtagaa
     2161 gtagctcgcc gcgctggcat tgatgatgcg caggcgcacg cgctcgccgg gtctgccctc
     2221 gatgagtgtc tggcgctggc cgttgatgag gaaggcgtga tagcccacgt cggagacgtc
     2281 catcggcatc atgcggtccc actcgcgctg gaagtagtcc ttcaaattcc cgcgctgcca
     2341 ggctccgagc acggactgcg cgttgcgccg catcagcccg aagtaatcgc tgccccgcat
     2401 gagcatgcgc atgacctcgt gcgggttgaa gttggtccag tcggagagca ccagcacctg
     2461 ctcgcggtcc gccctcaccg gctcgccccc gcgcggctcg acgacgatgc tgccatagac
     2521 gccgctctgc tcctggaggt gcgtgtgcga gtggtaccag taggtgccgc tgtgccgcag
     2581 ctcgtattca aaggtgtgcg tctttcccgg caggatgggc ggcgtggtga catgcggcac
     2641 gccgtcctgc tcattgggca gcagcaggcc gtgccagtgg gtggaggtgg tctcccgctt
     2701 gaggtggttg tgaacccgga tgcgcgccca ctcgccctcg cggaagcgca gcgtggggcc
     2761 gggaatgccg ccattgatgg tgaagccgcg caccgccttg cccgccgggc tgacggtttg
     2821 ctcgcggatg tgcagatcgt attccaccac gcgcgaactg gcggggatgc gtgaagtctg
     2881 gcagacgtcg caggccagcg cggccatgga aggcccgaat agggcgggaa gaagaaagcg
     2941 tttcatggga atcgagtcgt tgcggttcac ggaaccaagc ccgcatgcgc tgatgtccct
     3001 cagggaccgc gaggcgcggg cacaatgcgt ctgtcagaga cgccggctca attcaggaag
     3061 gagcagaaaa gcaccgccag ccggacctgc ggctgattct cgaagctgct ttccgcccag
     3121 cgcgatgcgc cctcattcaa agcccgtgga gagcgtggaa gtggtcctgc cacttccaca
     3181 ggcacccaaa ccgcctgcgg cgcccatttg cgaccgctga cgggcggggc attggccggc
     3241 tcagcaggcg cggagggctc cgcgcattcg cacaggctca tggcggcgca gcactccatg
     3301 gcgcacgctt ccagttccgc cgtgcgcggc atcgccagca caggccaggc ctgggccagc
     3361 agcgcgaaaa ggcagacgat ggagcgaagc agggtcatgg gttcatccgt gctgacgcaa
     3421 ggttggagaa agagttacac cttttattcc cccatgcctc ctttttccgg ctcgccatga
     3481 aggatgccgt gccgggtgat gcccgctcat catgaagtcc atgcccaaat gctatttccg
     3541 gtatcgcact ccggcgctta atgactgccc gcatgtccat acaagctccg cgcaaacagc
     3601 atctctgggt ctggtttctc tggctcgccg gtttctacgg cgtctggacc tggctggtct
     3661 tcggcaatga ccgctggggg gatgtgaccg cgcactggcc gatcgccctt gcgatggcgg
     3721 cgggcagtta tgtggcgggc tccaccccga tgggcggagg tacggtgggc tttccgatcc
     3781 ttgtgctgct cttcggcctg ccggccagcc tgggacgtga cttcagcttc gcagtgcagt
     3841 ccataggcat gaccagcgcc agcattttca tcctctcacg ccgccagccg ctggcttggt
     3901 ccatgctcaa agggtcggtg tttggcgcgg tgctcggttt gccggcaggc ctgtttttca
     3961 tcgctcccta tgtgccggag ctgtggatca agctcgtttt tgccgtcatc tggtgcagct
     4021 ttggcgtgct gcacctttat cgcatcggcg agatcgcctc ccacgacggc atgacggaat
     4081 ttgacgagcg ctgggacttc aaggtcggca cggtgctggg catcttttcc gcagccaccg
     4141 ttgcctcggt gacaggcgtg ggcattgaca tggtgctcta cgccacgctc gtgctgctct
     4201 gccgcgcgga cttgaagatc gccatcccca cctcggtcat catcatggcc ttcacctcgc
     4261 tgctcggcgt gcttgtcaaa aacctgacca ccggcatgca gccgggcgtg tatgagaact
     4321 ggctggcagc ggcgccggtg gtggcgctgg gcgcgccgct cggcgtgttc atggtcaatc
     4381 tcatcggacg caagccaacc ctgatcttcg tggccgtgct gtgcgtgggc cagtttgtct
     4441 ggacctgcca caccgagcgc gccgcgctgg gcatcccggg cttcattctc gccctggccg
     4501 ctgtcggcgg ctgcctgctc gtttttgaga ggctgcgtgt ctggggcggc gtgctcgtcg
     4561 gcgaggccaa agcgaagcac gcggctggat catccgccgc ggatggcagg caggaggacg
     4621 ccacccactc gaaagaagca gccgcaacct gtctgcaagg ctgagctggg cggtctcacc
     4681 tgcgcccgcg ccgcggatga agcgctcatt ttttccggga atgcagtgcc agtgtgcggc
     4741 tggctcggag aactgacagg ttcacaaggc tgcgggcgga cgacattcgg attcatccag
     4801 ggagggaagc cccagatcat gcggaatgaa ttggtggccg gggggatcac ttcttctccg
     4861 cgtccggctt gcacttgcac agccactccg gggcctggca cccgtcgcag gtggcggtgt
     4921 tgtattcaaa gcgggcggtg acggccttgg cgtcggcgtt gtctttgtag cgcaggatga
     4981 gccactcgcc gggtttcacg atgggaagga caaagccctt cgcgtccttc gtgacctcca
     5041 gcttcaccgg cttcgcgcgg tcgccggtcg tggcggtgag ggactgcttg tccatggcga
     5101 cgggcttcat gtccttgccg agcacggcga tgtgaatcgt gccgcctttc accgtgacct
     5161 cgccgtgcag ggtttcgttg ttgctgaatt cgacgatgcg gccgccgttc gggccgagtt
     5221 cgacgccttc atggctgaag gcgaggctgg tgatgagggt gacgaggagg ctgagtttgg
     5281 ttttcatggg tgggttggtg tttgggtggg tggtttgccg aagagaaaaa agacggcggg
     5341 tgtgaccatg aggtcgagca gggtgctgct gacgaggccg ccgacgatga cgacggccac
     5401 gggatgcagg atttccttgc ccggctcgtg cgcggcgagc agcagcggga tcaaagcgat
     5461 gccggcggcc agcgcggtca tgagcacggg cacaagccgc tccagcgtgc cgcgcacgat
     5521 catggggcgg ccgaaggttt cgccctcatg cttcatcagg tgcaggtaat ggctgatcat
     5581 catgatgccg ttccgcgccg ccacgccgcc gacggcgatg aagccgacga gggtggcgat
     5641 gctgatgtta tccaggagca gccaggtgag cgcgaggccg cccatgagcg cgagcgggat
     5701 gttcagcatg acctgcaagg cgagcgagag actcctaaag tagccgaaca gcaggatggt
     5761 gatggcgagc agcacgaggg caaagtagaa ggcgatgcgc ttcgtggcgg cctgctgcgc
     5821 ctgaaactcg ccctcgaagc gcaggaagca gccttcgggc agtttcacct tctcgcgcac
     5881 gagcttttcc cactggcgca cgaggctttc caggtcgcgc tcggaggtgt tggcgccgat
     5941 gacgatgcgg cgctgggtgt tttcgcggtt gatgacgttc ggacctttgc cttcgcggat
     6001 gtcggccacg aggttgagcg ggatgagctg gccgctgtgc gtctcgatga gcaggttgcc
     6061 aattttctcc ggcgagtcgc gccacgactc cggcaggcgc agggcgaggt ccatcgtgcg
     6121 ctggccctcg cgcagctcgg ccatgacctt gccgccgagc agcgtggaga cctgcgcatt
     6181 gatgtcaccg ggctggaggt cgtaagcgag ggcacgctcg cggttcacct cgatcttgat
     6241 ctgcgggatg ggcacctggg cctccaggtt cacatcggtg agtccgggga tggactggcc
     6301 cagatcgcga acctgcgcgc ctttctccct cagcacgtcg agatccgggc cgaagatctt
     6361 cacggcgatc cttgccgaga cgccgctgag catgtggctg agccgatggc caatgggctg
     6421 gccgacattc acaaaggtgc ccggaatggc ggagagcagc ctgcgaatct ccgcgaagac
     6481 gacctcacgc ggcctgccgc tctcgtggaa ctcgacatcg aactcattca cgctcaccgg
     6541 catgacgtgg tcatcccgct cggcacgtcc ggcgcggcgg cccacgcttt tgacttcctt
     6601 gatgctctgg agcaggcgca cgcccacctc gccaatctca ttggattgcg ccagggaggt
     6661 gccgggagcg ctcacgagcg agatggtggc gctgccttca ttgaaggcgg gcaggaagtc
     6721 cttccccatc atcggataca gcatcagcgc ggcgatcaac agcacgccgg caaaagccag
     6781 gacgagcatg ggtgcgccaa gcgccacctt gagaaaggtg cgctgcacga tccacttcat
     6841 cgcgcggatg acaaaaccat cgtcgtgggc ttttccgccc gctttgagca gcagcgagca
     6901 caaaaccggg atcaccgtga gcgaaacaac aaacgacgcc gccatgctgg tgatcgtggc
     6961 gatggcaatc ggcgtgaaaa gccgcccctc gatgccttcc agaccgagca acggcaggaa
     7021 gacgaggatg atgagcacgg tggcgtaaag gatggagttt cgcacttcgc tggaagcgga
     7081 ggcgatggtt tcaaggtgat gcgggcactc ctgcccgcga tgagcgcctt ctttgcgggc
     7141 aggagtgccc gcaccacttt ctcgcagacg gcggaagacg ttctccacat ccacgatggc
     7201 atcatccacc accatgccga tggcgacggc gaggccgccg agcgtcatgg agttcacact
     7261 cacgccgaag gccttgaagg tcaggatcgt gacggcaaag gacaacggca tcgccatgag
     7321 tgtgatggcc gtggtgcgca ggctgagcag gaacaggaag aggatgatcg tgaccatgat
     7381 ggccccgtca cggatggcct ccttcagatt gccgatggcg tgctcgatga agtcgccctg
     7441 gcggaacagg acctcgacgg tcacaccttc cggcagcgtg ggccgaaact cttccagcgc
     7501 ggcctcaatc tgctgcgtga gcgtcagcgt gtcaaaaccg ggcgctttat caatgctgag
     7561 gattacgccc atgttgccat tcacgctggc atcgccgcgc atcgtctgca cggcgtggcg
     7621 cacctccgcc acatcgctga tgagcaccgg gcggctgccc acggtcttga tgacggtttt
     7681 ggcgaggtca tcaaggctgg tggtcatgcc gagattgcgc accatgatct cctgcggccc
     7741 ggcttgcaga taaccgctcg tcgcattccc cgccgcacgg cccaccgcct cctccacctc
     7801 cgccaaagtg atgccaaagg cggccaggcg gtaaggatcg ggctgcacct ggatctgttt
     7861 caccccgccg ccgatggtga gcacctccgc gatgccgctg atgctctgca aacgacgccg
     7921 cagcgtccag tccgccaggg tgcgcaagtc catgggcgag atgtaaccgg gctcgcccgg
     7981 ctttttcgag cagcgcagcc ccacgagcag aatctcgccc atcagcgatg aaaccggcgt
     8041 caggccgggt ttcaccccct cgggcaaggt ggagagcatg ccctgcatgc gctcctgcac
     8101 gagctggcgg gcacggtaga tgtccatgcc ccagccaaac tccgcaaaga tgagcgacag
     8161 gcccacatcc gagttcgagc gcagcctgtc gagcccgccg acgccctgta ccgccgcttc
     8221 gagccgctgt gtgacgagcg cctccacctc ctccggcgcg aggccgggcg cctcggtgag
     8281 gatcgtcacc gtcggcttcg tcatgtccgg cagcacctcc accggcaact gggtgagtgc
     8341 ttgataaccg aacaccaacg tcaccagcgc cagcatcaat atcagaggcc ggtgatgcag
     8401 ggagaagcgg atgagagagt tcagcatgcc accacctccc ggcgtttgaa ggcggtgacg
     8461 atcaacagca gcagcagcaa gccgctcgtg gccgcgaaaa aggtcgtgag tgtgctccac
     8521 tcgttgtgag caccttcgtg atcatgatcg tggtccccgt gctccgcggc ttgctgctcc
     8581 tttgtcatct ccgtgccgtc ctcgttgtgc ggatgaccgt gagcggcatc catggcttct
     8641 ttgaggctca cgctgccttt gcccgcaaag gcgagcgcat aggcaccgcg tgtgacgact
     8701 tcatcgccgg gcagcaggcc gctgaccact tcgacaaagc ggtcgttctg cgccccgagc
     8761 acgaccagag ccttcacgaa ggcatttttc agctcatagt ccttcacata gacgaagcgc
     8821 tgcgcgggat cgccctgcac ggcgccgcgc gggatggaca tcacgttctc acgctcgctc
     8881 acgacgatgc tgaactccgc cttcatgccg ggacgcagca gcttgtctgg attcgtgacg
     8941 tgaaacgccg cctcgatggt gccggtggcc acatccgcct cgacggcgat gtgaacgagt
     9001 ttggcctcga agaccttgtc cggcagcgcc tggagccgga tgtgggcttt ctggtccagt
     9061 ttcagcttgg ccgcgaaatg ctgcggcaca tgcgccacgg cctcgacggt gctgagatcg
     9121 aggatttcga tcaacgcgtc gtccggcgac accggctggc cttgcgcgag gttcaccttc
     9181 gagacgaatc cggcgatggg agcctccagc ttcaccacgg gcggcggatc gcccggctgg
     9241 cggctttcca cccacgccac ctccgccccg gcatccactt cctgatgcgg gatgaccagc
     9301 acggaaaagg cccggcccgg aatgcggctg ctgacgacgc cccgtttccc cggcagcacc
     9361 tcaatgctgc cgagcgcaaa aaccgtctcc tcaaacacgg tctcttccgc ctcggcgaat
     9421 tcgaggttca tgttcgagac cgccacatca tcaaggatga tgtccggcgt ggcagcgaag
     9481 gccccggtca cgagaagcag aaggcaaaag atgggtttca tgggctttca gagctcgacg
     9541 cccgcctttt tcagcagggc catggtgagg aagtagagga tgaccgtgag cacgatgccg
     9601 gcgcccagcg cgggccagaa gccgccgagc tttttcacca gccaaggcat gagcagaaac
     9661 atcggcagcg tcggcaggac gaaccagaag gtgccgaaag cgtgacgccc gatgagagcc
     9721 gagtccttcg tctcgctgta gagccagatg aaggcgagca gcgaagtgag cggcagcgag
     9781 tggatgatac ttgcggcggc gttgttgcgc ttggcgattt ccgtgacggc gacgatgacg
     9841 ccggcggaga ggaagagctt aaggaggtaa taggtcatgg tgttttggga tggatgggtt
     9901 cagggcgagg tggcggcctc gtagcggatg cgcgcgaggt gaaagtcgcg ctcggcatcc
     9961 agggcggcgg attcgagctg gaggcgctgg tcgcgggagc gcagcacgct gaggaggtcc
    10021 gcctgcccat tttcataggc tttctccatt gcagccgagt gctgcacggc caggggcaca
    10081 agcgtgtcgc gcatctcgcg cacaatggag gcgttggcct ccatctcgcg gcgcgcggtg
    10141 ccggcctcgc catggatctg tgccgcgagg gcttcggctt ccaaacgggc gcgctcggcg
    10201 ctggcggccg tttcggtgat ctcgccctgg ttgcggttcc acaacggcag cgggatggag
    10261 aaacggaagc cgatgaagcc tgtgctctcc ctttgcgcgg aggtcacatc ctgcatctca
    10321 tgcgcgccaa agatccccgc agtgacatcc gggatgcgtc tggccctggc gagttgctgg
    10381 ccggtctgcg cggcctccgc cttcgtctgg gcaaggcggt aatccggccg ctgctgccag
    10441 gatgatgcgg gcggcagctc cagcgcgggc aagccgcccg tgagggtcag cggctcttcg
    10501 gagcgcaggc cgagcatggg cttgagctgg cccagcaccg ccacgcttgc ggcctccagc
    10561 agacgggact cgatgcgcag cttctgcgcg tcgagctgca cctgcctggc atccagcgga
    10621 gagacctcgc cggcctcggc acggtccgag gcaaagtccg ccagcttttg cgccagctcc
    10681 atctgctgct ggcggagctg gcgctgcctg gagagcgtca gcagccgcac cgcctggctt
    10741 ttcgcctcgg caatgaggcg gcgctcggcg tccctgacct ccagctcggc ggccgcgacc
    10801 acttgggagg tcagcttctt ttccaaactc agccgccgtg tgagcgggaa ggactgatcc
    10861 agcgaaacca cgcctgtctg cgggctgagg ttgctctggt tttgaaaatc atagcccagg
    10921 gagggattcg cgagccggcc cgcgccgagc tggcgtcccc gcgcctcctc cacggccagg
    10981 cgcgcggctt tgagggcggg atgatgcgta cgcgctcggg atgcgacttc atcgagagac
    11041 agagtgagcg ccaaagaggg gctggcgcag gcccatgcga gcaggaaaag agaaggtggt
    11101 ttcatggcga aaagagttca gtgacccggg catggaaatc ccatgcggga aggcgggcgg
    11161 acgcatcaca gccattcggc catccgtcgt gggatggctg gctgcgcgcg tttcaaactc
    11221 tcagcacaat cgttctcgtc aggatctgcg gccactcgcg cggcccgtca cgccggcctt
    11281 tgtcaggcgg ttcttccctg cgccggcacg ccggattcag gcggaaaagt cctggctccc
    11341 atgccaccga ggccagcgcc agaagcacag gcgcctgcgc actgtatgcc tcgtgatgct
    11401 tggccactag atcatccacc acggcagtgt gcctgtgcgt gtcctcatcc tccccgtcct
    11461 catgatgatg cggcacgtcg tgatcctcat cctcgtggca gtcatgggag tgcgggccgt
    11521 ggcagtggtc caccaaagtg atccgctcca cgccgccgca gtcgcacaac catccgtgat
    11581 gaatgccaaa ggtctgcgcc caggccaccg ccagagtggc ggtgagcatc agcagggcat
    11641 ggaccgcgca gcgcattgtt ttgaaacttg ccggtccgtt tcaccgctgg caagcggaaa
    11701 gtctcgccgt cagttcgccg cgcgggtttg ctccgtcttt accgcgccgc tttttccgcc
    11761 gccgagcggg cggcgaggat gaaatcaatc cgagttggcg ccttctccaa ctgactgctg
    11821 taagcgtcca gctcattggc caggatcagc cagcggtcat gggtgtcgcg gtccagaggc
    11881 ttgaggggtt ccagggcatg gccggggttg gcttcgaagt actggccgtg caggtttttg
    11941 ccgcgctggc ccggcttttc cggcatggga ttcagcccgt gaagtccgcc cgccgtgccc
    12001 caccactgca cggcctcgaa gtgcggatgc cccggcggca catcgctgac ataaagggtg
    12061 gccgagccgt ccttgtgcag gcgcttttgc agttcgccca ccggcaccgc gtccacagcg
    12121 agctttgtcg ccgccgccat ggccgccgca tgacccgccg cctgacctag agacatccag
    12181 atgggttcca gccgcagggc gcagaagccg acatgcgagg atgacaccgc cacgggcacc
    12241 agcaggtttt ccaactcgtt gggcacgagc gcgccgtagg ggacttggta aggcggcacg
    12301 ggattgtaaa attccccgcc atgactgccg ccgatgagag ggcctttgtg gctggtgccg
    12361 tggcagttgt tgccgtagtc gcccatggcg atggcgtcct tgaagagttt ggcgcgcgcg
    12421 tcgccgggcg cgtgctcgct gtcctgctgc acatagacgt ggcggccggt catgcgtcgc
    12481 gcttcgcgca cataaagctg cgggggcagg tggccggtct cggtgaactc gtccttgcac
    12541 cagccccact gcgacgctgg ctcgcggaac ctggccggca ccgcctcgtc attcgccagg
    12601 aaaaagagca gcccggcatt gtagcgcagg tgctcgtcaa agatggcctg ccgggcggcg
    12661 gcatcaccct cgggccaggc gaggttggcg ccgggcatgg agaggcggat gagtccgcgc
    12721 gagacatcgt tgatgtcgta tttccggttg ggcagcgcgg gcggctgcgc tttgacgacg
    12781 catcccgagg ggtagccgaa gacgctttga attttccctg attcgaggat gggcagcacg
    12841 tcgagaaaat cctcccgccg gtagcctttc ggcgcctgga tgggcgcgcg gttggcggga
    12901 tcgtcggtgg cgcagaagcg gaagttgtag gcctgaagtt ccttgtcgct tttctccggc
    12961 gccagtgact cgccatagac atcacgccct tcgcgcccga catggtaacg cacccccgcc
    13021 gcggccatca ggtcgccctc gtagctgccg tccacgaaaa cggccgcgcg aaccatgaaa
    13081 gtctccgcgc cctgggcgaa ctccgcctgg ctgatgaccg cgcgcgcgac ttgcacagac
    13141 ttgagcgccg cgcctttgcg cagggtgacg cgctcctgct ccgcgagcat ttgctccagc
    13201 acgagcaggt tcaccctggg ttcgccaaag acgccttcat ggctgtcctt cacctgctgc
    13261 gagtccgcgc cataggttcg ctcgtagtgc gccttcacac gcttgctgaa ttccaggaac
    13321 gagccggtca gggactcgcg gctgtgataa tccgtgtgcg agaggccgga ggcgagcagc
    13381 cccccgatgc gcgcggttgg ctccagcaaa agcaccgtcg agcccgagcg cgccgccgcc
    13441 acagccgccg cgatgccgcc gggcgtggct ccgtaaatgc agacgtccac ggtcaactcg
    13501 gcggcggaca cgggcagggc gaggatcagg gcggtgaaaa gcgggcgcat ggcgcatggg
    13561 agaccgcccg ccagcgcggc gcaagtggca acaggtgcgg gcaaagaaac cgcgccgtta
    13621 ttttttgtcg ccgccgccga agagtttgcc cacggacttg gtggcgcgct ggaagaaatt
    13681 gggctcctcg gcggacttgg tgctgcgctt gcggtagccg ccggaggcga cggagctgcc
    13741 accggtccag tcaggcttgt cggactcgcc cgccaggccc atggcgatga gtgtgggcac
    13801 gtcgaggaag ccgttggcgg ccaggccgct gcggacttgg aaaagcagga tggcgttgtg
    13861 ggtgttcttg ccctccttgc cgtctagggt ggaggtgtag aggccttttt ccttgagcag
    13921 gccctgcgcg cggtagagca ggtgcttctt accgttcgcg ctgtagccgg aataaggcgg
    13981 cgtgggatac atctgctcca cagtccagta gccggcggcg ggctccgtca cgggggcctg
    14041 cggcggaatg acaggcccca cgaatttgtc gggcgtgatg acctccggct cgggcggctt
    14101 ttcctgcaca atgagcggcg gcgaggtggc gacgacattg gcaatgtccg ccggcaaccc
    14161 ggcaggcgcg gcggcgggtg tcatggccgg cggcgcggct ggcgcgg
//
LOCUS       DAOMYJ010000130        14148 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Prosthecobacter sp. isolate AMR_MDS_5629
            NODE_1445_length_14148_cov_22.869723, whole genome shotgun
            sequence.
ACCESSION   DAOMYJ010000130 DAOMYJ010000000
VERSION     DAOMYJ010000130.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867850
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prosthecobacter sp. (wastewater metagenome)
  ORGANISM  Prosthecobacter sp.
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Verrucomicrobiaceae; Prosthecobacter.
REFERENCE   1  (bases 1 to 14148)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14148)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.30
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:34:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,742
            CDSs (total)                      :: 4,684
            Genes (coding)                    :: 4,676
            CDSs (with protein)               :: 4,676
            Genes (RNA)                       :: 58
            tRNAs                             :: 55
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14148
                     /organism="Prosthecobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1445_length_14148_cov_22.869723"
                     /isolate="AMR_MDS_5629"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1965333"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..229
                     /locus_tag="PLP58_13690"
     CDS             <1..229
                     /locus_tag="PLP58_13690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ09625.1"
                     /translation="PARRPPAAMQPSSEAAERAAQMAAVFRRRQMMSNLGWTLACLIA
                     LGALAWIVWRDHYQNAATEPPKVESMENSAQ"
     gene            complement(337..3768)
                     /locus_tag="PLP58_13695"
     CDS             complement(337..3768)
                     /locus_tag="PLP58_13695"
                     /inference="COORDINATES: protein motif:HMM:NF013866.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative Ig domain-containing protein"
                     /protein_id="HRJ09626.1"
                     /translation="MKTPFAILCLLFCVLTAQAQTLPGFSFNTGDNSFTYDDPDDPGA
                     VVTGYIRKPTTAAASGQAILICHGKGGSAANFNSQHAANFVQWGYYCIVPQLSHAGAG
                     GGTSSNEGYCPENSRIGRACLKILADVPTVDTTRVAMFGHSMGAYFTGGFCGENPSPY
                     PALRAAILGAGGSQGTGGSSTNALPTQAEVAGITAPMLLFHGTQDTSYPRSQDLKASL
                     DANSVPNRLLLYQAVPHGIMDANQKRADVYSIGRAWFTTHGVLSFAGNTAPTISAPAS
                     VTVTAGVASSPVAVTIGDGQTAASALTVEAFSLDADVAGTTTPPVPAYSGRLNNSGLV
                     WGGSGANRTLTITPAAGVTGTVEVALVVTDGTVSTGQLATVHYLQVTITSGAPPLTLA
                     YNTGGTRINVNRNATPGSSLTATGGTAPYSWALVSGSLPAGITLNSNGTFSGTSTASG
                     GHTFRARATDTLGATGEADFSLWVNATRQGTVTPITFNGPVTGAPVTFSLYLPPGYSG
                     GSASYPIVYHLHGIGGTHDGPQITTVPVSHEAAVSAGLIEPCIIVFPDGYNDSFWADA
                     INVVKPAETNIMQEIIPHMEANYRVRPGGNHRVIQGFSMGGFGAAKFATKFPASFACC
                     AVYDGAMLTWTQMQQRHANQTANIFNNSMAAYNDHSAFHWVVQNAATLRDSMPFRDAV
                     GDLVSENQAWKAALDAQSIPSGYVETGLPHSVGPLLDAQGANMWEFIAAALAAAADDF
                     TISFPGGGAELAASTGSAMNVTLSASGGTAPYTWSVLSGALPAGVTLSSAGVLSGTPT
                     ATGTFTFTVQVSDSGGGTDTQQLVLTVNAPPAITPQASRDVNGNIVLAWPTTIGAWYQ
                     VEVSDDLMNWLPLGVAVAATQTAMSWTDNGTLTGTPPATDDRRFYRVRDFGVFTVNVS
                     GNNFTYTDAQRSVTGVLRTPTGSGSFPAVIINHGTSGTAAGYAGARANEMLPWGLVCI
                     GANLTHQDANGPEVDLGHSPENHARIEACLAVLASRGYVDMNRVVMFGNSRGGFTTAG
                     SASLLHSRIKAIGICAGGVLEDGDGSDVSYPSVSEASGITAPALIFHGSNDTVVPPAN
                     SLRLQNLLSDLGVTNSRILHATTGTASHNLHQDATVWPQVLGAWQAWLVTHGVVP"
     gene            complement(3778..6531)
                     /locus_tag="PLP58_13700"
     CDS             complement(3778..6531)
                     /locus_tag="PLP58_13700"
                     /inference="COORDINATES: protein motif:HMM:NF012958.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase-fold protein"
                     /protein_id="HRJ09627.1"
                     /translation="MKYSALFHTSLAAALLMPAFGDEPQRTPRDPSGGIFFYAKEGYD
                     SNTAPVANPDVLGNWVQFYWSEIEKEKGRYDWSLIESRMKPWVDAGKKVAFRVYWIGS
                     GYWKNPAARTATPQSVWDDGAKFAKHADSGTEIPLPWDPIYQRRALAFLTALAERYDA
                     NPNVLFFDVTPGAETNPYRFVAFNQRTPEFKDQYAATPASDGRKYSDELWLATVRGWI
                     DATDRIFKHLPLVVTLNVGTLNLGDARNDYSVEIGQYAVDHGFYVGQNGLHGASYIED
                     SGRKEAFLKWSKQTRIVFETLGDAGTSTQYGKKPLGSLREIVDAANRVNAHYLLPYPV
                     DVLKGTKGQADYDPEFAAALAHGARTLGQGGKTTTQETSQVAPGNRARDSEPSWLMPP
                     VKGPNLHYKTFDSKAAGQKVSYLLYLPPGYETSGQRRYPVVYWLHGMGGSQQGVPAMT
                     ARLTSAIEAGKTPPLIMVFVNGMIRSSYVDSADGKIPVETVSIQELIPHVDATYHTLA
                     TRAGRMIEGFSMGGGGAAKWGFKHPELFGSISIIDGALHKPDDPTAGRLASSFQTIYG
                     GDRAYFEASNPWNLAQKNADKVKGRTPVRIVTSTKGLGPLNKEFSGLLTRLAIAHEFH
                     AIPDAPHSPGPLYEGLGDENWTFYQSAFSTAPTAPKVSQAAITLTAPKSVSLAANTTK
                     GFQLDGERWTYRDGDFAMSGILLKPEGRGPFPAVLISHGLGGSAESFGMNKAREMVKW
                     GFVCIAPGYTHTGGARGAAKGAPQPADYGASAENIRRAKTCLELASQMPEVDAKRLFA
                     YGHSMGGFVTIGLAASEPGLLKAAAITGSGVAPRAGYPAPSTEAAAKIRTPFLMLHGA
                     ADNVVRPEQSESLKKVLDQNKVPNDRLVADGQGHPIDQTMREEVFRLIREWFTKHDAP
                     KAK"
     gene            complement(6528..8069)
                     /locus_tag="PLP58_13705"
     CDS             complement(6528..8069)
                     /locus_tag="PLP58_13705"
                     /inference="COORDINATES: protein motif:HMM:NF012958.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase-fold protein"
                     /protein_id="HRJ09628.1"
                     /translation="MNRQDELFAAYLDGTITAAEERELAAWMAGSAEHARAFVEACLR
                     EQQLTAAVRASARRRSVEIVHQRAAEPPPSSRPSKAFRRLLPLAAAAAVVAGWMGWQV
                     WDPSPVAAYLSGRLGGVTVERAGEKLSANEHQPLSEGDLIQTRTGGAIVSFAGEDTRF
                     DLGADARVRVRSLRGRKEFQLLSGTVSAEVAPQRRGAMLWLTDDARAEIVGTKFTLAA
                     AGWFTRLDVTEGAVRFSRHGDSEPTLVKAEQFAAADVQGTTGACSIDEFETAPWSVPG
                     RRTPGFEHVTFRSNRLDREIGVNVMLPPGYDRDRRGGYPVLYLLHAMNGDEHSEAARL
                     GTALRSAMEDRRLPPCLVVAPNSGPAFPREPMFANVMVARELTRFVDSRYPTRAQRGM
                     RVLCGLGYGAQQAVILSLFHTDVFALGCAVDDTLRGGSPGFRRLGIHHRPRPRIQPQL
                     LLLHTDDHGDDARALADFLNETGVETDVTALAARHLDDEALPTVFVEALATRLQLLWN
                     RPNAR"
     gene            complement(8066..8596)
                     /locus_tag="PLP58_13710"
     CDS             complement(8066..8596)
                     /locus_tag="PLP58_13710"
                     /inference="COORDINATES: protein motif:HMM:TIGR02989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HRJ09629.1"
                     /translation="MLSDAQQELFTRLWTSVQPAVSGYIHALVRDDPVADDLTQETAL
                     ALLRTFSSYDPQRPFLPWALNTAKFQILGHRRDEARCKVLFDGDLLDRITENWGDTAP
                     QISGEQAALGECLETLAPHARKLLRLRYDEALDSTQIARHLATTAAAVRMALQRVREQ
                     LRICVERRLQTRGGLT"
     gene            complement(8686..10203)
                     /locus_tag="PLP58_13715"
     CDS             complement(8686..10203)
                     /locus_tag="PLP58_13715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ09630.1"
                     /translation="MRRSLSCRLKASLQLATLLLFPLSLSAIEPGYEALDLYTASSPS
                     SSRDTTWKPGDGVTLEVSGMAWIGPDRLGVTVRKGEVWFLDGVLGEDPAKITYHRFAS
                     GLHEPLGLLQDGDSYLVAQRAEVTRLRDTDGDGSADEYLTYARGWNVTGNYHAYTYGP
                     ERDGEGRLWITANLGMGERANNKLPWHGWAMLADGKGGVIPMAAGLRSPCGLGANLEG
                     DMFVTDQQGTWVAATPVHHLRKGVFYTNQEALPTLDHPDSPIKLTAPLPKDCAYPEAL
                     RAAPVMAPATVWLPYNKMGRSGTDIQVIDQKGKFGPFDGQLLVGEFTNSAISRVFLEK
                     VGGEYQGACFPFLSGFPAAVVRMAFAPDGSLFVGMTNRGWSSLGNRSYGITRVRQKGA
                     IPFSICEMRARPDGFELALTEPADPATAASPASYTLESFTFTYSGAYGSDEIEKRPHT
                     IESAAVSPDGLTVRLKVAGLRELYVHELRASGLRSKQGEPLLFPDAYYTLNKIPR"
     gene            10792..10998
                     /locus_tag="PLP58_13720"
     CDS             10792..10998
                     /locus_tag="PLP58_13720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ09631.1"
                     /translation="MKSDAHEGATLNYIHHNPVKHGYVGKWTDWPWSSAREYVNEVGR
                     EAALEHWKAYPVDEYGAGWDDASL"
     gene            complement(11109..13223)
                     /locus_tag="PLP58_13725"
     CDS             complement(11109..13223)
                     /locus_tag="PLP58_13725"
                     /inference="COORDINATES: protein motif:HMM:NF012264.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HRJ09632.1"
                     /translation="MRSQTSIFDFRFSILLILATSLHAEPIVTGFERFHNGEPTREGG
                     ALLYNELGCANCHGGDTGLPARQGPVLADLKSRANAAWIAEFLTDPQSAKPGTMMPAL
                     FDGLPAKEKAAQIQAVTHYLMTLKPVGKPVKPKAARHANAERGSARFHQVGCVACHAP
                     TPDFHPAHGTPKPEDFTSRPVPLPDLKKKYSLLTLAAFLENPDLVRPDGRMPHLGLNA
                     EDAMDIACHLMDYRPSDPREAPGLPTLKLNAGLAKKGAEIVAKLNCASCHSLTAEDKP
                     VTVALQKNEGGCLSSKPAAGRPHYDLSDRQRAALNAFLSGKDQPAFTTTQQTHLTLQA
                     LNCYACHDRDGIGGPDVARNRYFVGDEGIADAGRLAPPLTGIGQKLRADWLESVFLGE
                     GRVRPYLQTKMPKYPAHAKTLTALLKKTDKKELPKLHPEGDLAAGQKLTGILGGMNCI
                     TCHVWGDKPSLGIQALDISQLDKRLTPEWFRDYMLNPQGYRPGTLMPPLWPGGQSMLK
                     EVLGGDTEKQLAAIWEFIKKGDGLPEGYPAHVANAFELIPQDRPIIQRTFLNHVGSQA
                     ILAGFPGGIHIAYDGDKGRPALAWRGRFFDANSTWFVRAAPFEDPLEKEVFAWPQGSE
                     NDAPGYRGYRLDKAGNPTFLTRVDGVEVEETYRVEEGKLHRTVTWAQGQKEPAWTHPE
                     GLEVTDSAANVNHQRHFVYSWK"
     gene            complement(13289..14119)
                     /locus_tag="PLP58_13730"
     CDS             complement(13289..14119)
                     /locus_tag="PLP58_13730"
                     /inference="COORDINATES: protein motif:HMM:NF013429.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HRJ09633.1"
                     /translation="MKYLLSSALYGDMKLAQVLPEVAPAGCAGIDIWGKQHATHREEV
                     DEMGVDAFEALLKKHGVQLVCSTRYPLGPFGLQPEMPMVKRLGGSLIVCASKGPKNVT
                     GAEAKAGIKEFLELMKPHADAAAEHGITLAIENHGNALLYSPDSIRYWAELNTHPALG
                     VAFAPHHLHDHIEDMPKLIRELGAKNLPFIYFQEYGIGSKQTVAKEIELQQLPGYGTL
                     DYKPILAAMRDVGFNGYVEIFMHPTPRGIPMLPTPGEITAAINKSRDHIEGLLKEVNA
                     "
BASE COUNT         2455 a         4292 c         4742 g         2659 t
ORIGIN      
        1 cccggcgcgc cgtccgcccg cagccatgca gccctcatcg gaagcagccg agcgcgccgc
       61 gcaaatggcc gctgtgtttc gccggcggca gatgatgagc aatctgggct ggaccctcgc
      121 ctgcctcatc gccctcggcg cgctggcgtg gatcgtgtgg cgggaccatt atcagaacgc
      181 cgcgactgag ccgccgaagg tagagagcat ggaaaacagc gcgcaatgag gatgtgattc
      241 gcattccgcc ccagtggcgg ggctggttca catgcggccg cgtctgcctg cgccccggcc
      301 agggaaacgg acatcaatct ttcgtcgcca cgccgttcat ggcaccacgc catgcgtcac
      361 cagccatgcc tgccacgcgc cgagcacctg cggccagaca gtggcgtcct ggtgcagatt
      421 gtgggaggcg gtgccggtgg tggcgtggag aatgcggctg tttgtcacgc cgaggtcgga
      481 gagcaggttt tgcaggcgca gcgagttggc gggcggcacc acggtgtcgt tgctgccgtg
      541 gaagatgagc gccggcgcgg tgatgccgct ggcttcgctc acgcttgggt aggagacatc
      601 gctgccgtca ccgtcctcca gtacgccgcc cgcgcagatg ccgatggcct tgatgcgcga
      661 gtgaagcagc gacgcgctgc ccgcagtggt gaagccgcct cggctgttgc caaacatcac
      721 cacgcgattc atgtccacat agccgcgcga ggcgagcacg gcgaggcagg cctcgatgcg
      781 ggcgtgattt tccggcgagt gtcccagatc gacctccggg ccgttcgcgt cctgatgcgt
      841 gaggttcgcg ccgatgcaca caaggcccca gggcagcatc tcattggcgc gggccccggc
      901 gtagcccgcc gcggtgccgc tggtgccgtg attgatgatg acggcgggaa aggagccgct
      961 gcccgttgga gttcgcaaga cgcctgtgac gctgcgttgc gcgtcggtgt aggtgaagtt
     1021 gttgcccgac acattgaccg tgaagacgcc aaagtcgcgc acacgataaa aacggcggtc
     1081 atcggtggcg ggcggcgtgc cggtgagcgt gccgttgtcc gtccagctca tcgccgtctg
     1141 cgtggcggcc acggccacgc caagaggcag ccagttcatc aaatcatcgc tgacctccac
     1201 ctgataccat gcgccgatcg tcgtcggcca tgcgaggacg atgtttccat tcacatcgcg
     1261 cgatgcctga ggtgtgatcg ctggcggcgc gttcaccgtc aaaacgagct gctgcgtgtc
     1321 cgtgcctccg cctgagtcgc tgacctgcac ggtgaacgtg aacgtgcccg tcgccgtcgg
     1381 cgtgccgctc aagacgcctg cgctgctgag cgtcacaccc gctggcaacg cgccgctgag
     1441 gacactccag gtgtagggtg cggtgccgcc gctggcgctg agcgtcacat tcatcgcact
     1501 accggtgctc gccgcgagtt ccgcgccgcc gccggggaag ctgatggtga aatcatcagc
     1561 ggcagcggcc agcgcggcgg cgatgaattc ccacatgttc gcgccctgcg cgtcgagcaa
     1621 cgggccgacc gaatgcggca gtcccgtctc gacatagccg gagggaatgc tctgcgcgtc
     1681 gagcgcggcc ttccacgcct ggttctcgct caccagatcg cccaccgcgt cacgaaacgg
     1741 catggagtcg cgcagcgtgg ccgcgttttg caccacccaa tgaaaggcgg aatggtcgtt
     1801 gtaggctgcc atgctgttgt tgaagatgtt cgcagtctgg ttcgcgtggc gctgctgcat
     1861 ctgcgtccac gtcagcatcg cgccgtcata caccgcgcag caggcgaagc tggccgggaa
     1921 cttcgtcgcg aacttcgccg cgccgaagcc gcccatcgag aatccctgga tcacgcgatg
     1981 attgccgccg gggcgcacgc ggtagttcgc ctccatgtgc ggaatgatct cctgcatgat
     2041 gttcgtctcg gcgggtttca ccacgttgat ggcgtccgcc cagaacgagt cgttgtagcc
     2101 atcgggaaag acgatgatgc acggctcgat gaggcccgcg ctcaccgccg cctcatggct
     2161 caccggcacc gtggtgatct gcgggccgtc gtgcgtgccg ccgatgccgt gcagatgata
     2221 aacgatggga taactcgcgc tgccgccgct gtagcccgga ggcagataca ggctgaacgt
     2281 caccggcgcg cccgtgacgg gtccgttgaa ggtgatcggc gtgaccgtgc cctggcgcgt
     2341 ggcattcacc cacagtgaaa aatccgcctc gcccgtcgca cccagcgtgt ccgtggcccg
     2401 cgcgcgaaac gtgtggccgc cggaggccgt gctggtgcct gaaaacgtgc cgttgctgtt
     2461 cagcgtgatg cccgcaggca gcgatcccga cacgagcgcc catgaatagg gcgcggtgcc
     2521 gcccgtcgct gtcaaagaac tgcccggcgt ggcattgcgg ttcacattga tgcgcgtgcc
     2581 gcctgtgttg taggccagcg tcagcggcgg agcaccgctg gtgatcgtca cttgaagata
     2641 atgcaccgtc gcgagctgcc ccgtgctcac cgtgccgtcg gtgacgacga gcgcgacctc
     2701 gaccgtgcct gtcacacctg ccgcaggtgt gatcgtgagc gtgcggttcg cgccgctgcc
     2761 gccccacacg aggccgctat tgttcagccg gcccgaatac gcgggcaccg gcggtgtggt
     2821 cgtgcctgcg acatcggcat cgaggctgaa tgcctccact gtgagcgcgc tggccgctgt
     2881 ttgaccatcg cctatcgtca ccgccacggg cgacgatgcc acgcctgccg tcaccgtcac
     2941 ggaagcaggc gcgctgatcg tcggcgcggt gtttccagcg aaggaaagca cgccatgcgt
     3001 ggtgaaccac gcgcgaccga tggaatacac gtccgcgcgc ttctggttcg catccatgat
     3061 gccgtgcggc accgcttgat acagcagcag ccggttcggg accgagttcg cgtcgagact
     3121 ggccttcaaa tcctgcgagc gcggatagct cgtgtcctgc gtgccgtgaa acagcagcat
     3181 cggcgcggtg atgcccgcca cctcggcctg cgtgggcagc gcgttcgtgg aactgccgcc
     3241 cgttccctgc gatccgccag cgccgaggat ggccgcgcgc agcgcgggat acggcgaggg
     3301 attctcgccg caaaagccgc cggtaaaata cgcgcccatg ctgtggccga acatcgccac
     3361 acgcgtcgtg tccaccgtcg gcacatccgc gagaatcttg aggcacgcgc gaccgatgcg
     3421 gctgttctcc gggcagtagc cctcgttgct cgaagtgccg ccacccgcgc ccgcatggct
     3481 gagttgcggc acgatgcagt aataacccca ctgcacaaaa ttcgccgcgt gctgcgagtt
     3541 gaagttcgcc gcgctgccgc ccttgccgtg gcagatgagg atcgcctgcc ccgaggccgc
     3601 cgccgtcgtc ggtttgcgga tgtagcccgt caccaccgcg cccggatcat cgggatcgtc
     3661 gtaggtgaac gagttgtcgc ccgtgttgaa gctgaagccc ggcagcgttt gcgcttgcgc
     3721 cgtcaacacg cagaacaaca ggcagagaat ggcaaaaggt gtcttcatga cagcacttca
     3781 cttcgccttg ggagcatcgt gcttcgtgaa ccactcgcgg atgaggcgga agacttcttc
     3841 gcgcatggtt tgatcaatgg gatggccttg gccatcggcc acgaggcggt cgttgggcac
     3901 cttgttttga tcgagcacct tcttgagcga ttccgactgc tcggggcgca ccacattgtc
     3961 cgcagcaccg tgcagcatca aaaagggcgt gcggattttc gctgccgcct ccgtcgaggg
     4021 cgcgggatag cctgcgcgcg gtgcgacgcc gctgccggtg atcgccgcag cttttagcag
     4081 gcccggttct gacgcggcga ggccgatggt gacaaagccg cccatgctgt gaccgtaggc
     4141 gaagaggcgt ttcgcatcca cctcgggcat ctggctcgcg agttcgagac aggtcttcgc
     4201 acggcggatg ttttccgcgc tcgcgccgta gtcggcgggc tgtggcgcgc ccttcgccgc
     4261 gccgcgcgcg ccgccggtgt gtgtgtaacc cggcgcgatg cacacaaaac cccacttcac
     4321 catctcgcgc gccttgttca tgccaaaact ctccgcgctg ccgccgaggc cgtggctgat
     4381 gagcacagcc ggaaacggtc cccttccctc cggcttgagc aaaatgccgc tcatggcgaa
     4441 atcaccgtcg cgataagtcc aacgttcgcc atcgagttgg aagcctttcg tggtgtttgc
     4501 ggccaaagac accgatttgg gcgctgtaag ggttatggca gcctgcgaga ccttcggggc
     4561 cgttggagct gtggaaaaag cgctctgata aaacgtccag ttttcatcac cgaggccctc
     4621 gtagagcggt ccgggcgaat gcggcgcgtc gggaatggcg tgaaactcgt gcgcgatggc
     4681 gaggcgcgtc agcagcccgc tgaactcctt gttgagcggg cccagcccct tcgtgctcgt
     4741 gacgatgcgg accggcgtgc ggcccttcac cttgtccgcg ttcttttgcg cgagattcca
     4801 cggattgctg gcctcgaagt aggcgcggtc gccgccgtag atcgtctgga acgagctcgc
     4861 gagccgtcca gcggtcggat catccggctt gtgcagcgcg ccgtcgatga tcgagattga
     4921 gccgaagagt tcaggatgct tgaagcccca tttcgccgcg ccaccgccgc ccatggagaa
     4981 accctcaatc atgcggcccg cgcgcgtggc gagcgtgtga tacgtcgcat ccacatgcgg
     5041 gatcaattcc tggatgctca ccgtctccac cggtattttt ccatcggcgg agtccacata
     5101 gctgctgcgg atcatgccgt tgacgaagac catgatgagc ggcggcgtct tgcccgcttc
     5161 gattgcgctc gtgagcctcg ccgtcatcgc gggcacgcct tgctgcgaac cgcccatgcc
     5221 gtgcagccag tacacgaccg gatagcgccg ctgccccgat gtctcgtaac ccggcggcag
     5281 ataaagcaga tagctcacct tctgccccgc cgctttgctg tcgaaggtct tgtaatgcag
     5341 atttgggccc ttgaccggcg gcataagcca gctcggctcc gaatcacgag cacgatttcc
     5401 cggcgcgact tgcgacgtct cctgcgtcgt cgttttgcct ccctggccga gcgtgcgcgc
     5461 accatgcgcc aatgcggctg caaactccgg atcgtaatcc gcttggcctt tcgtgccttt
     5521 gagcacatcc accggatacg gcagcaaata atgcgcgttc acgcggttcg ccgcgtccac
     5581 gatctcgcgc agcgatccca gcggcttctt gccatattga gtgctcgtgc ccgcgtcgcc
     5641 gagcgtctcg aagacgatgc gcgtctgctt cgaccatttc aggaacgcct ccttgcgccc
     5701 gctgtcctcg atgtagctcg cgccgtgcag gccgttctgg ccgacgtaga agccatgatc
     5761 caccgcgtat tggccgatct cgacggaata atcgttgcgc gcatcgccga ggttcagcgt
     5821 gccgacattc agcgtcacca cgagcggcag gtgtttgaaa atcctgtccg tcgcatcgat
     5881 ccacccgcgc acggttgcca gccacagctc gtcggagtat ttgcggccat cgctcgcggg
     5941 cgtggcggca tattgatctt tgaattcggg cgtgcgctgg ttgaaggcga cgaagcgata
     6001 cgggttcgtc tccgcgccgg gcgtcacgtc gaaaaagagc acattgggat tcgcgtcgta
     6061 gcgctccgcc agcgcggtga gaaaggcaag cgcgcgccgc tggtagatcg ggtcccacgg
     6121 cagcggaatc tccgtgcccg agtccgcatg cttcgcgaac ttcgcgccgt cgtcccacac
     6181 cgactgcggc gtggccgtgc gcgcggcggg attcttccag tagccgctgc cgatccaata
     6241 cacgcgaaac gccaccttct tccccgcgtc cacccacggc ttcatgcgcg actcgatcag
     6301 gctccagtcg tagcggccct tttccttctc gatctcgctc cagtaaaact gcacccagtt
     6361 cccaagcacg tcaggattgg cgacaggcgc ggtgttcgag tcgtagccct ccttggcata
     6421 aaaaaagatg ccgccggagg ggtcgcgcgg tgttcgctgg ggttcgtctc cgaaggcggg
     6481 catgagcagc gcggctgcca ggctggtgtg aaaaagggcg gagtatttca tcgggcgttg
     6541 ggtcggttcc agaggagttg gaggcgcgtt gccagggcct cgacgaacac ggtgggcagc
     6601 gcttcgtcgt ccagatggcg cgcggcgaga gcggtgacat cggtctcgac gcccgtttca
     6661 ttcagaaagt ccgccagggc gcgggcgtcg tcgccgtggt cgtcggtgtg cagcaaaagc
     6721 aattgcggct ggattctcgg acgcgggcga tgatggatgc cgagacggcg gaagccgggg
     6781 ctgccgccgc gcagcgtgtc atccaccgcg cagccgagcg cgaagacatc ggtgtggaaa
     6841 agggaaagga tcacggcctg ctgcgcgcca tagccgagcc cgcacagaac tctcatgcca
     6901 cgctgcgcgc gggtggggta acggctgtcc acaaagcggg tcagttcgcg tgcgaccatc
     6961 acattggcaa acatcggctc gcgcggaaac gcggggccgc tgttgggggc gaccacgagg
     7021 cagggcggca gccgccggtc ctccatggcg gagcggaggg cggtccccag acgcgccgcc
     7081 tcactatgct cgtcgccatt catggcgtgc agcagataca gcacaggata gccgccgcga
     7141 cggtcgcggt catagccagg tggcagcatc acattgacgc cgatctcgcg gtccagccgg
     7201 ttgctgcgaa aggtgacatg ctcgaatcca ggcgtgcggc gtccaggcac actccacgga
     7261 gccgtctcga attcatcaat ggaacacgcg cccgtcgtgc cttggacgtc ggccgcggcg
     7321 aactgctcgg ctttgaccag agtcggttcg ctgtcgccat gccgggagaa acgcaccgcg
     7381 ccctcggtca catcgagacg ggtgaaccac cctgcggcgg cgagggtgaa cttggtgccg
     7441 acgatctccg cgcgggcgtc atcagtgagc caaagcatcg cgccccggcg ctgcggcgcc
     7501 acctccgcgc tcaccgtgcc gctgagcaat tggaattcct tgcgcccgcg caacgagcgc
     7561 acccgcaccc gcgcgtcggc gcccaggtcg aagcgcgtgt cttcgccggc aaaactcacg
     7621 atggcgccgc cggtccgggt ttgaatgagg tcgccctcgg aaagcggttg gtgctcgttc
     7681 gcggaaagct tctcaccagc gcgctccacc gtcacaccac cgaggcggcc gctgagatac
     7741 gcggcgaccg gcgatggatc ccagacctgc catcccatcc aaccggccac gacagcagcg
     7801 gcagccgcca gcggcagaag acggcggaaa gccttcgagg gacgtgaaga tggcggcggc
     7861 tctgccgccc tctggtggac gatttcaaca gaacgccgcc gagcggaggc gcgcaccgcg
     7921 gcggtgagtt gctgctctcg cagacaggct tcaacgaaag cgcgggcgtg ttcagcgctg
     7981 ccggccatcc acgcggccag ttctctttct tccgccgcgg tgatcgttcc atcaaggtag
     8041 gcggcgaaca gttcgtcctg gcggttcatg tgagacctcc tcttgtttgc agacgacgct
     8101 ccacgcagat gcgcagttgt tcacgcacgc gctgcagagc catccgcacc gccgcggcag
     8161 tggtggcgag atggcgcgcg atctgggtcg agtccagggc ctcgtcatag cgcaggcgca
     8221 gcagtttgcg ggcgtgcggc gccagtgttt cgaggcactc gccgagcgcc gcctgctcgc
     8281 cgctgatttg cggggcggtg tcaccccagt tctccgtgat gcgatccagc agatcaccgt
     8341 cgaagagcac cttgcaacga gcctcgtccc ggcgatgacc gaggatctgg aatttggccg
     8401 tgttcagcgc ccaaggcaga aaggggcgct gcgggtcgta agaggaaaag gtgcgcagca
     8461 aggccagcgc ggtttcctgg gtcaggtcgt ccgcgaccgg gtcgtcccgg accagcgcgt
     8521 gaatgtagcc agaaaccgcc ggctggaccg aagtccagag tcgggtgaaa agttcctgct
     8581 gcgcgtcgga gagcatgtgt gcgccctatc tttccaggac gcggacacag tgtaacagcg
     8641 tttttgattt tgtttcggcc cgtggatgcc tggcgaaaat gaaactcagc gaggaatctt
     8701 gttcagcgtg taataggcgt ccggaaaaag cagcggctcg ccttgcttcg agcgcaggcc
     8761 ggaagcgcgc agctcgtgga cataaagctc gcgcaatccg gcgactttga ggcgcacggt
     8821 gaggccgtcg ggcgagacgg cggcggactc gatggtgtgc gggcgctttt cgatctcgtc
     8881 gctgccgtag gcgcccgagt aggtgaaggt gaagctctcc agcgtgtaac tggccggact
     8941 cgcggcggtg gcggggtcgg cgggctcggt gagagcgagt tcgaagccgt ccggcctcgc
     9001 gcgcatctcg cagatggaaa aggggatggc acccttttgc cgcacgcggg tgatgccgta
     9061 gctgcggttg ccgagggagg accagccgcg gttggtcatg ccgacaaaca ggctgccatc
     9121 cggcgcgaag gccatgcgca cgacggcggc ggggaagccg ctgagaaaag gaaagcaggc
     9181 gccttgatac tcgccgccga ccttttccaa aaacacgcgg ctgatggcgg agttggtgaa
     9241 ctcgcccacg aggagctggc cgtcgaaagg gccgaacttg cccttttggt caatgacctg
     9301 gatgtcggtg ccgctgcggc ccattttgtt gtacggcagc cagaccgtgg ccggagccat
     9361 gactggagcg gcccgcaggg cctcgggata agcgcagtcc ttgggcagcg gggcggtgag
     9421 tttgatcgga gagtccgggt ggtcgagcgt gggcagcgct tcctggttgg tgtaaaagac
     9481 gcctttgcgc agatgatgca ccggcgtggc ggccacccag gtgccttgct ggtccgtgac
     9541 aaacatgtcg ccctccaagt tcgcgcccaa tccgcaaggg ctgcgcagac ccgccgccat
     9601 ggggatgacg ccgcctttgc catcagccag catggcccag ccgtgccatg gcagcttgtt
     9661 gttggcgcgc tcgcccatgc cgagattcgc ggtgatccaa aggcggcctt cgccgtcgcg
     9721 ctcggggccg taggtgtagg cgtggtagtt gccggtgaca ttccatccgc gcgcataggt
     9781 gaggtattcg tcggcgctgc cgtcgccgtc cgtgtcgcgc aggcgcgtca cttccgcgcg
     9841 ctgggccacc aggtaagaat caccgtcctg gagcagcccg agcggctcat gcagcccgga
     9901 ggcgaaacga tggtaggtga tcttggcggg atcctcaccg agcacgccat ccagaaacca
     9961 aacctcgccc ttgcgcacgg tgacaccgag gcggtcgggt ccgatccatg ccatgccgct
    10021 gacttcgagc gtgacgccat cgccgggttt ccacgtcgtg tcgcgcgagc tggaggggga
    10081 tgaggccgtg tagagatcga gcgcttcata gcccggctcg atggcggaga gggagagcgg
    10141 gaagagaagg agggtggcaa gttggagtga agcctttagg cgacacgaga gagaacgtcg
    10201 caacgcagct ttcccgggac aagctgaagc ttgaactcca acgggatgaa ctccaacagt
    10261 tggagtgaag cctttaggcg acatggaagc gcgctccgct gcgggagttt cccagaacaa
    10321 gttgaagctt gaactccaac ggtttgaaaa tgaatcttga cgatctgcat cgctcgaaac
    10381 agccttggat tcatgtaccg ctggcggagg ctgtccgagg atgaacggag gattgaactg
    10441 gcctttcgca agcgccagca gcggccctgg catagtttga agcatcgcga cttgggagcg
    10501 ggcgcttatc tcatcacggc atcctgccac gagcactctc ccatcattgg gcactcggcg
    10561 gaaagaatga cgcactttgc ggaatctctg ctgcagactc ttgatgaggc cgccgatgag
    10621 acgaaggcgt gggtggtgct gccgaatcat tatcatgttc tggttatttc aaaggctgtc
    10681 ttggaagtgc tgaaagcatt ggggaaattg cacggaagga cttctttcga atggaacgga
    10741 gaggagggga agcgagggcg caaagtgtgg ttcaacgcgg ctgagacacg catgaaatca
    10801 gacgctcatg aaggcgcgac gctgaactac atccatcaca accctgtgaa acatggttat
    10861 gtgggaaagt ggacggactg gccgtggtcg agcgcgcggg agtatgtgaa cgaagttggc
    10921 agggaagccg cgctggagca ctggaaagcc tacccggtgg acgaatatgg tgctggctgg
    10981 gacgacgcct cgctttaaac cttggagtga gtgttggagt gaagtcttta ggcgacacgg
    11041 gagtgcgctc cgctgcgggc ggtcattgaa caagctgaag cttgaactcc aacagcttga
    11101 actccggctt atttccatga atagacaaag tggcgttgat ggttgacgtt ggcggcggag
    11161 tcggtgactt cgagtccttc ggggtgcgtc caggcgggct ctttttgccc ctgcgcccag
    11221 gtgacggtgc ggtgcagttt gccttcctcg acgcgataag tttcctcgac ttcgacgcca
    11281 tccacgcggg ttaaaaaggt ggggttgccg gctttgtcca gccgatagcc gcgatagccg
    11341 ggcgcgtcat tttcggagcc ttggggccag gcgaagactt ctttttcgag cgggtcttca
    11401 aacggcgcgg cgcgcacgaa ccaggtggag ttggcatcga agaaacgccc gcgccaggcg
    11461 agcgccgggc ggcctttgtc gccatcgtag gcgatgtgga tgccgccggg gaaaccggcg
    11521 aggatggcct gggagccgac gtggttgagg aaggtgcgct ggatgatggg ccgatcctgc
    11581 gggatgagtt cgaaggcgtt cgcgacatgc gcagggtagc cttcgggcag gccgtcgcct
    11641 tttttgataa actcccaaat ggcggcgagc tgcttctcgg tgtcgccgcc gagcacttcc
    11701 ttgagcatgg actggccgcc gggccaaagc ggaggcatca gcgtgccggg acggtagcct
    11761 tgcgggttga gcatgtagtc ccggaaccac tcgggcgtga ggcgtttgtc gagctgggag
    11821 atgtccagcg cctggatgcc gagcgaaggc ttgtctcccc acacatggca ggtgatgcag
    11881 ttcatgccgc caagaattcc ggtgagtttc tggccggcgg cgaggtcgcc ttcgggatgc
    11941 agcttcggca gttccttttt gtcggtcttc ttcaacaggg cggtgagggt tttggcatga
    12001 gccggatact tcggcatctt ggtttgcagg taaggccgca cgcgtccctc gccaaggaag
    12061 acagactcca gccagtcagc gcgcagcttt tggccgatgc ctgtgagcgg cggcgcgagg
    12121 cggcccgcgt cggcgatgcc ttcatcgccg acgaagtagc gatttcgcgc cacatcgggt
    12181 ccgccgatgc cgtcgcgatc atggcaggcg tagcaattca gcgcctggag tgtgagatgc
    12241 gtctgctgcg tggtggtgaa ggcgggctgg tctttgcccg agagaaaggc gttgagcgcg
    12301 gcacgctggc ggtcgctcaa atcatagtgc ggacggcctg cggcgggctt ggaggaaagg
    12361 cagccgccct cgtttttctg gagcgccacg gtcacgggtt tatcctcggc agtgagactg
    12421 tggcaggagg cgcagttgag tttggcgacg atctccgcgc cttttttggc taggcctgca
    12481 ttgagcttga gagttggcag gcccggagcc tcgcgcggat cactgggacg gtagtccatc
    12541 aggtgacagg cgatgtccat ggcgtcctcc gcgttgaggc cgaggtgggg catgcggcca
    12601 tcggggcgga ctagatcggg gttttcgaga aaggcggcca gggtcagcag gctgtacttt
    12661 ttcttcaaat ccggcagcgg caccgggcgc gaggtgaagt cctccggctt cggcgtgcca
    12721 tgagcgggat gaaaatccgg cgtgggcgca tggcaggcca cgcagccgac ctggtgaaag
    12781 cgcgcgctgc cgcgctcggc gttggcgtgg cgcgcggctt taggtttgac gggcttgccc
    12841 acaggtttga gcgtcatcag ataatgcgtc accgcctgga tctgcgcggc tttttcttta
    12901 gcgggcaggc cgtcaaaaag cgcgggcatc atagtgccgg gcttggcgct ctgagggtcg
    12961 gtgaggaatt cggcgatcca ggcagcgttg gcgcggctct tcagatccgc aagaacgggt
    13021 ccctggcgtg cgggcaggcc ggtgtcgccg ccgtggcagt tggcacagcc gagttcgttg
    13081 tataaaagcg cgccgccttc gcgggtcggc tcgccgttgt ggaaacgttc gaagccggtg
    13141 acgatgggct cggcgtggag gctggtggcg aggatgagta aaatcgaaaa tcgaaaatcg
    13201 aaaatcgaag tctgacttct catgagggcg attttctgga gatgcgtgaa aattttcgaa
    13261 gttcgggctt cgaatttcga gcttttcctc atgcgttcac ctccttgagc agtccttcga
    13321 tatggtcgcg ggatttgttg atggccgcgg tgatctcacc cggcgtgggc agcatgggga
    13381 tgccgcgcgg ggtggggtgc atgaatatct cgacgtaacc gttgaagccg acatcgcgca
    13441 tagctgcgag gattggtttg tagtcgagcg tgccgtagcc aggaagctgc tgaagctcga
    13501 tctccttcgc cacggtctgc ttgctgccga tgccgtattc ctggaagtag atgaagggca
    13561 ggttcttcgc gcccagctcg cggatgagct ttggcatgtc ctcaatgtga tcgtgcagat
    13621 gatgcggcgc gaaggctact cccagggcgg gatgcgtgtt cagctcggcc cagtagcgga
    13681 tggagtcggg cgagtagagc aatgcgttgc cgtggttctc gatggcgagc gtgatgccgt
    13741 gctcggccgc agcatccgcg tggggtttca tgagttcgag gaactccttg atgcccgcct
    13801 tggcctcggc tccggtgacg tttttcggtc ccttggaagc gcacacgatg aggctgccgc
    13861 cgagcctttt caccatcggc atctccggct gcaagccgaa ggggccgagc ggatagcgcg
    13921 tcgagcagac gagctgcaca ccgtgttttt tcagcagggc ctcaaaagca tccacgccca
    13981 tctcatccac ctcctcgcga tgcgtggcgt gctgtttgcc ccagatgtcg atccctgcgc
    14041 aaccggcggg tgccacctcc ggcagcactt gagccagttt catgtcgcca tacagcgcgg
    14101 aggagagaag gtatttcaat tgcagcggcg ccttgaaact ggctcaag
//
LOCUS       DAOMYJ010000131        13959 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Prosthecobacter sp. isolate AMR_MDS_5629
            NODE_1458_length_13959_cov_22.246404, whole genome shotgun
            sequence.
ACCESSION   DAOMYJ010000131 DAOMYJ010000000
VERSION     DAOMYJ010000131.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867850
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prosthecobacter sp. (wastewater metagenome)
  ORGANISM  Prosthecobacter sp.
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Verrucomicrobiaceae; Prosthecobacter.
REFERENCE   1  (bases 1 to 13959)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13959)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.30
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:34:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,742
            CDSs (total)                      :: 4,684
            Genes (coding)                    :: 4,676
            CDSs (with protein)               :: 4,676
            Genes (RNA)                       :: 58
            tRNAs                             :: 55
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13959
                     /organism="Prosthecobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1458_length_13959_cov_22.246404"
                     /isolate="AMR_MDS_5629"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1965333"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            569..724
                     /gene="infA"
                     /locus_tag="PLP58_13735"
     CDS             569..724
                     /gene="infA"
                     /locus_tag="PLP58_13735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012420273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HRJ09634.1"
                     /translation="MFRVKLKNGHEVLAHISGKMRKRFIRLVIGDEVKIEMSPYDMDK
                     ARIVYRL"
     gene            730..1278
                     /locus_tag="PLP58_13740"
     CDS             730..1278
                     /locus_tag="PLP58_13740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009965114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease H-like domain-containing protein"
                     /protein_id="HRJ09635.1"
                     /translation="MARDIVYFDLETRRTANDVGGWGNKHRMGISVAVTYSTRLGQYR
                     IFEEEETADLIHQLVRADLVVGFNHVSFDYEVLMGHTIFDFRDQVRDLDLLVDLEKKL
                     GHRLKLEAVASASLGTGKTADGLDALKWWREGRLADIAEYCAYDVKVTKCVHEYGAAH
                     GFVRYLDRNNREQKVAVDWSLD"
     gene            complement(1289..2269)
                     /locus_tag="PLP58_13745"
     CDS             complement(1289..2269)
                     /locus_tag="PLP58_13745"
                     /inference="COORDINATES: protein motif:HMM:NF025341.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartyl protease family protein"
                     /protein_id="HRJ09636.1"
                     /translation="MTRLTPLFFCLLLTACAVTHSHGPVPARVSRALEKTAMTRAEFE
                     AARSTRSAPRGGSVVVPFKLEDSTPVFAARVNGMEVPMILDTGAASTMLHAATALRSG
                     VRILESKAGVVAMQGVIGQEQARIGLLSRLEAGGWMLEGQPCLVRMHENRVPLEDGEP
                     HNLLGFDVLARHCSHVTLDYPKEQAVFAFGGLYQPQRGVHTARAPFETLLGAPFIRVS
                     SGGKSWQAVVDTGSFNGVEISQEVAEMLGVEKQGRPVEGIVLVSVGGTVTSEKAGLRT
                     LRLAEARLFGETWRGAQVDISPGPPRVGGFFLKDYRVTFDFRRRCVWLEW"
     gene            2372..3607
                     /locus_tag="PLP58_13750"
     CDS             2372..3607
                     /locus_tag="PLP58_13750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007417460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HRJ09637.1"
                     /translation="MRTALTAVLLLSSLPLAAENWPQWRGPRMDGTSHDQGFPVAADS
                     QSLAWKVSLPGAGHASPIVWGDRVFTVTAVAETQQRMLLCHDRADGKLLWQSVVLTSP
                     MEGKHRLNSHASSTPATDGERVYTAFLDGSESVVSAHDFTGKPVWQVRPGVFASKHGF
                     CSSPILFEDKVIVNCDHDGPGYIVALSRADGRELWRIQRPNQTRSYCVPLIREMAGRT
                     QMVLSGSKCVASYDPRTGALLWIMDGPTEQFVASLVQDDKSGLLLMTGGFPEHHILAI
                     KPDGTGDVTRSHIQWRTNKGVAYVPSPICESGWFLVVSDSGIGHCFDAATGEIVWEER
                     MREHHASLVSAEGRVYFLNGFGTLRAVRPGKSYELLAESEAGEKVMASPALSEGQIFI
                     RGEKNLICLGKRRQSAAAR"
     gene            complement(3697..4716)
                     /locus_tag="PLP58_13755"
     CDS             complement(3697..4716)
                     /locus_tag="PLP58_13755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009959803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HRJ09638.1"
                     /translation="MSFPAEPAASLPETATEAEVAGLLGGARERIFKEVGKVIVGQHE
                     VVDQMLITLLAGGHCLITGAPGLAKTLLVKTVADVFDLSFHRIQFTPDLMPSDITGTE
                     ILEDTAEGRQLVFNKGPVFASMILADEINRTPPKTQAALLEAMQEHQVTVAGNTLRLP
                     EPFFVLATQNPIEMEGTYPLPEAQLDRFMLNIVIDYLGEDEEVAVVTRTTGVKGEPVQ
                     RLFTGAQLQAFQQLVKKVPVAEDVARYAVRLAAASRPKRPGAPDFVNEWVSWGAGTRA
                     SQNLVLGGKTRALIQGRAHVTFDDIRALAQPVLRHRILVNYRAEAEGVTVEKVIARLL
                     ETVKQ"
     gene            complement(4855..8454)
                     /locus_tag="PLP58_13760"
     CDS             complement(4855..8454)
                     /locus_tag="PLP58_13760"
                     /inference="COORDINATES: protein motif:HMM:NF038134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choice-of-anchor M domain-containing protein"
                     /protein_id="HRJ09639.1"
                     /translation="MKIPRILAALGLLLSAQALPAHEGHSHGLSSYTAGHGDIGVHWH
                     DGELELHMHLDAGSVVNGAVLAAGIEADPDETLIVASDAAKQTATAAISAGTGVAEGV
                     PFWVLPASNNPALPFLGFATEELDPAEWNSNIHFKVKDVHSPSGSGHFSVYQSDGLGG
                     FDFLVSTALGGLTEDDRISLSPGTHDHFFIAFSEPGLWEVTVQAEGEHGAEGVVESVE
                     VTFLFQVGPFRWTAGHGDIGLAYEEGGELELHLHLHEGAVVNGAAQEADSEPPADQTV
                     IVVSETAQQVATAAIASGAGVSEGGTVWILPTAPNAELPFLGFGTEELEPAEWESDLT
                     FSLVSVSSPSGTGHFSVYQSDGLGGFDFVMSTALGGITEDDHFEMPAGTHDHSFVAFS
                     EPGLWAVTLKASGEHHDDGEVESDEVTFYFEVAEETAGRIALSQAAYTYNQGSTLATV
                     TLNRTGAVTATSVTVNTSNGTAQTTNPPFAAGVAGTDFTALTAPATTVDFAEGETSKT
                     VDIALLPRTGNVPNTRFNVTLSNPTNDVSLGLSSAEVRVLANDSMNPTVRIAAPAANA
                     SLSLAAPVETRGTAGDKNGVSRVEVVLNGGEPIETELSEDSRPNAVYWHADIIPAEGA
                     NTLEVTSFDMSGNASTTATRSFTFSRRWLLAVGRFELHHDDDDHAHDGDDGHGHGDGS
                     GHLDPGDEAGTLMLVTLPRTAFTALTPRDSHLQSSQVLPGTIVALAAKAKSGYLFSHW
                     EGVPAGANIQGATIAFVMPAANVPRIRAVFAENPFFGLGTRASFLGLLHPEHEEDATT
                     LDSVGQVTGTVVTNTGAFSGKVFIGGETRSFVAVVYASGAVMFNHDGELEPEFEFDGR
                     HMTMDFHDGEFHVHIGGDHDDHHHGDIASDPEDEEIHVAGTLLPAAFSKTSPAPAELP
                     GLFTLSLPSKDQSLPESDYPRGDGFANLTLSSDGKLRTVATLADGTKFTASTELVVEH
                     HHDDHGDPLPADPGEHEDEDHIIAPLFAQLRTPGGGPKDLGGTFAGELAFDTHEEDSD
                     VHGEDLVWIRPAVTELAGTTAAARATQLYTGGWPEGITVDAVGAHYDATIPVQDTLGL
                     GSASPTGNGQLHFAGGKLASDVLVTDFNITGSTAAKLPPTNRSFTLSFTPATGAFKGT
                     FTPNWEDKARALPVFQGILIQKGASQGGYGWFQSNRVGDLDPEAGGVTLGAQE"
     gene            complement(8540..10258)
                     /locus_tag="PLP58_13765"
     CDS             complement(8540..10258)
                     /locus_tag="PLP58_13765"
                     /inference="COORDINATES: protein motif:HMM:NF038134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choice-of-anchor M domain-containing protein"
                     /protein_id="HRJ09640.1"
                     /translation="MSSFILGFGVALPAAEATPPMDILYGHFEIHADYVLTPGNPDAG
                     WQLNVSYNKNDNFNDRTQIVRLDPETTTIIASPRTGMFDNGNPILITSAVSRLGPVGA
                     PLWFMPQNNVLGTPFMGARAVMAPGIFQTFFNGNYSPSATGSISLRLVSVTGTGPDAG
                     GQFGLWESDGQTLLFYFGPQTNNLIPTLPPNAHSHFNWGFTKLGSYFLTIEALGRLNP
                     QHGGQLTSTQKVFRFAVPFSSRLQGQATVRAGFVPAGKNFHLLVEDAADNVAYTPTQG
                     FLEASAAASGEAQTTLPGAARQMPLTFSTAGSPVANLVGLAPALAAQGLPAGALDGDA
                     VKLRLLSVSGPGHFAVLNADGTGLLMNSADGVDAADEITLSSGADLEALAVFTANGLY
                     RVTMELAGTQGGEPVKSGPMVLAFGANLTAAYTYAQWRDSFERTHGLPANTLANTRAD
                     YDKDGLSNGAEFQLFWHGCDPVKGDAGLLPKGRPEGDAAVMDFLRDTYKDTLNEKTFQ
                     QSPSTSPDMQTWTTRNARVTGRALETCETCAEAGNAYGRVMLRRLRVLDAPGERRFFR
                     FVFKPD"
     gene            10373..12619
                     /locus_tag="PLP58_13770"
     CDS             10373..12619
                     /locus_tag="PLP58_13770"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HRJ09641.1"
                     /translation="MLTAFLACHASFAQAQTEPLDLPPGLIELDMETGAPLDAAAAVM
                     PGEPELLQEVVVEAERVSAASGLTVGRAMRVAGIALQPVTEMSGEKLARRAQGTLGGT
                     LAWEPGVTSAYFGPGSSRPIIRGFDGFRVKMLRDDLSTMDLSDTSPDHGIGLEPLLAE
                     SLEVHRGPSALLYGNSAIGGAVNTRSRTLTRELPLSALTGGLDSRFESVSSGWAQTGY
                     FTLTHAPWVLQLTGSARESEDVRIPGRARTEAYEKAERPRVSNPGAGTVSPIPNPDGV
                     LPNTAHRSRSFSAGLSFLPDGPFWAGVSWSYFDSFYGLPYIFSGDATDFFGDSFIDLA
                     QSRFDLELGLDLDSGPLKKIQLRLAQSDYEHDELFEGRGRDSGAEFIETAMSKNTVEG
                     RLDFLHGGFGERLDGILGVHGFDEDFDASRLVVPPPAEFRVPSYFQTENLGAYLLEKL
                     RLFENWQLQLGARWERQRIVDDSLAGIVKARAQSDESLSHSLGLTWSTENLPGVKKLS
                     LTGIASKTERIPTATERYAFWNNAGLGRFLVGGDLDGTPLGLEKSTGFELGLAAEWER
                     ATLRLNGFHYDYENFIFLQEIPVFIQRAVEYIEREATIQGFEAELDLRLWQSGSRALQ
                     LTLMGDYVNGHNDTDDEPLPRIPPLRAGGRIEFTTSRLTLGLEARHSFAQDRLKPEPR
                     GELPTEGYTLVNADASWRLSKGRHDVLLTLQVTNLLNQDARQHTSFRKDVAPLPGIGL
                     VAGVRWTF"
     gene            complement(12764..>13959)
                     /locus_tag="PLP58_13775"
     CDS             complement(12764..>13959)
                     /locus_tag="PLP58_13775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009965039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015930 - glutamate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016638 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors [Evidence IEA];
                     GO_process: GO:0006537 - glutamate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glutamate synthase-related protein"
                     /protein_id="HRJ09642.1"
                     /translation="GTIALIALGCVYVRQCHLNNCPVGVATTDPKFRSKFKGKPEHVV
                     NFFNGVAHEVRGIMASLGVAKMDDLIGRPEFLRQRQVPGHKKASKIDLSRVLKDVGKD
                     LGQDAPRICLMNQNDGLDAHPLDDRIIQNAQFAIRDKAKVTPVRYKIKNTFRNIGTKL
                     SGEIAFHHGNHGLPPGSVDVTCEGSAGQSFGTFLCGGIKLTLVGEANDYVGKGLCGGE
                     IIIKPSPKAHPSFKSWENSILGNTVMYGATSGRLHAAGCAGERFCVRNSGATAVVEGI
                     GDHGCEYMTGGMAVVLGSFGKNFGAGMSGGVAYLLDESGAFHQLHNPEMIKGEPVTDP
                     EDINQLRKLITDHLEFTESQRAKDILDAWDTWLPKFVKVTSKAEPVQVPPEEPVAVEP
                     EAVKA"
BASE COUNT         2379 a         4544 c         4555 g         2481 t
ORIGIN      
        1 gggggggggg ggggggatgc gaagtggaat tcatgcggcc ggaggcggtg gttgaatttt
       61 gcggtttatt cctgacggcg ctgatttcat ccgatctcgg cgcttgtggc aagagtgatt
      121 tttggggggg atttgggttg gggccttgct catgcttccc tcataaggcc aggcgacacc
      181 cagacggaaa tccacatgcc gcgcctaact cccgcagcct tcgcgcatta tcgcggcaaa
      241 ttttccgtca tgtcacggcg aagccgcaga aggaaaataa gcggttgtca aagacgatga
      301 tggggacact cttggctcct cgttcttttt tccgtttccc tcccgacacc gcatgtccca
      361 tcaacgcccc tccaaccaag gcaaccgcaa ccgacctccc gcgcgcaagt cgagcgggcc
      421 gccgcgcggc attgtgacat cgcgccccgc tcctccacca cctcctcctc cgccgcgccc
      481 cgccgcgccg aaggaggagg tggagctgaa gcagaaggag gaggccatcg agcttgaggg
      541 cgtgatctcc gccgtgctcg ccggcaccat gttccgggtg aagctcaaaa acggccacga
      601 agtcctggcc cacatctccg gcaaaatgcg caaacgtttc atccggctcg tcatcggcga
      661 cgaggtgaag atcgaaatgt ccccctacga catggacaag gcgcgcatcg tttaccgact
      721 gtgaccggca tggccaggga catcgtgtat ttcgatcttg agacccggcg caccgccaat
      781 gatgtcggcg gctggggcaa caagcacagg atgggcatct ccgtggctgt cacctacagc
      841 acaaggctcg gccagtaccg catcttcgag gaggaggaaa ccgccgacct aatccaccag
      901 ctcgtgcgcg ccgacctcgt cgtcggcttc aaccatgtca gcttcgacta cgaggtgctc
      961 atgggacaca ccatcttcga cttccgcgac caggtgcgcg atctcgacct gctcgtggac
     1021 ctggagaaaa aactcggcca ccgcctcaag ctcgaggccg tggcctccgc cagcctcggc
     1081 accggcaaga ccgccgacgg gctcgacgcc ctcaagtggt ggcgcgaggg caggctggcc
     1141 gacatcgccg aatactgcgc ctacgatgtg aaggtcacca aatgcgtcca cgaatacggg
     1201 gccgcccacg gctttgtgag atacctcgac cgcaacaacc gcgagcaaaa agtagccgtg
     1261 gactggagtc tcgactgacc ccggccgctc accactccag ccacacgcaa cggcggcgga
     1321 agtcaaaagt caccctgtag tccttgagga aaaagccgcc cacgcgcggc gggccgggcg
     1381 aaatgtccac ctgcgcgccg cgccaggttt cgccaaagag acgcgcctcc gccaggcgca
     1441 gcgtgcgcag ccccgccttc tccgaggtca ccgtgccgcc cactgacacc agcacgatcc
     1501 cctccaccgg ccggccctgc ttttccaccc ccagcatctc cgccacctcc tggctgatct
     1561 ccaccccgtt gaagctgccc gtgtccacca cggcctgcca ggacttgcct ccagatgaga
     1621 ctctgatgaa aggcgcgccg agcagcgtct cgaagggcgc cctggccgtg tgtactcccc
     1681 tttgcggctg gtacagcccg ccaaaggcaa acacggcctg ctcctttggg tagtccagag
     1741 tcacatggga gcaatgccgg gccagcacat caaagccgag caggttgtgc ggctccccgt
     1801 cctccagggg aacgcggttc tcgtgcatcc gcaccaggca gggctgcccc tccagcatcc
     1861 agccacccgc ctccagccga ctcagcagcc cgatgcgcgc ctgctcctgg ccgatgactc
     1921 cctgcatggc caccacgccc gcctttgact ccaggatgcg cacacctgag cgcagggccg
     1981 tggccgcgtg cagcatcgtg gacgccgcgc ccgtgtccag gatcatgggc acctccatgc
     2041 cattgacccg cgccgcgaac acaggcgtgg agtcctccag cttgaaggga accaccacag
     2101 agcccccgcg tggagcggac cgggtgctcc gcgcggcctc aaactccgcc cgcgtcatcg
     2161 ccgttttctc cagcgcccgg ctcactctgg cgggcaccgg gccatgactg tgcgtcaccg
     2221 cgcaggcagt caggagcagg cagaaaaaaa gaggggtgag ccgtgtcacg cgagcgcctg
     2281 tatcaaagcc gcgccgccgg cgcaacggca aagcagcccg cccgcgcgca agactcttgc
     2341 aggcgcggcg cgccccggta ggctccgcgc catgcgcact gccctcaccg ctgtccttct
     2401 gctttccagc ctgcctttag ccgctgaaaa ctggccgcag tggcgcggac cgcgcatgga
     2461 cggcacctcg catgaccagg gttttcccgt cgccgcggat tcgcagagcc tcgcctggaa
     2521 agtcagtctc cctggcgcgg gtcacgcctc gcccatcgtc tggggggacc gcgtcttcac
     2581 cgtcactgcg gtggccgaga cccagcagcg gatgctcctt tgccatgacc gcgcggatgg
     2641 aaagctgctc tggcagtccg tggtgctcac ctctcccatg gagggcaagc accgcctcaa
     2701 cagccacgcc tccagcacgc ccgccaccga tggcgagcgc gtttacaccg cctttctcga
     2761 cggctccgag tccgtcgtct ccgcgcacga tttcaccggc aagcctgtct ggcaggtccg
     2821 ccccggcgtc ttcgccagca agcacggctt ctgctcctcc cccatcctct ttgaggacaa
     2881 agtcatcgtg aactgcgacc acgacggccc cggctacatc gtcgccctct cccgcgccga
     2941 cgggcgcgag ctctggcgca tccagcgccc gaaccagacg cgcagctact gcgtgcccct
     3001 catccgcgag atggccgggc gcacccagat ggtcctcagc ggcagcaagt gcgtcgccag
     3061 ctatgacccg cgcacaggcg ccctgctctg gatcatggac ggccccaccg agcagttcgt
     3121 cgcctccctc gtccaggatg acaaatccgg cctcctgctc atgaccggcg gcttccccga
     3181 gcatcacatc ctggccatca agccggacgg caccggcgat gtgacccgca gccacatcca
     3241 atggcgcacg aacaaaggcg tcgcctatgt gccctccccg atttgcgaga gcggctggtt
     3301 cctcgtcgtc tccgactccg gcatcggcca ctgcttcgac gccgccaccg gcgagatcgt
     3361 ctgggaggag agaatgcgcg agcaccacgc ctccctcgtc agcgccgagg gccgcgtcta
     3421 tttcctcaac ggcttcggca ccctgcgcgc cgtccgcccc ggcaaaagct acgaactgct
     3481 ggccgagagc gaggcgggcg agaaagtcat ggcctccccc gccctcagcg agggccagat
     3541 cttcatccgg ggcgaaaaaa acctcatctg cctcggcaaa cgccggcaaa gcgccgccgc
     3601 gcggtaagtc tcatccaacc ctccagtgcc tgtgccgcca gaagcgatcc tcagcttccg
     3661 gagtgccatc actttacgcg gggaaagcgg cgcgcttcac tgcttcaccg tctccagcag
     3721 ccgggcgatg actttctcga ccgtgacgcc ctcggcctcg gcgcggtaat tgacgaggat
     3781 gcggtggcgc agcacaggct gggccagggc gcggatgtca tcgaaggtga cgtgggcgcg
     3841 gccctggatg agggcgcggg tcttgccgcc gagcacgagg ttctggctgg cgcgcgtgcc
     3901 ggctccccag ctcacccatt cgttgacgaa atccggcgcg ccgggacgct tcgggcgcga
     3961 ggcggcggcc aggcgcaccg catagcgcgc cacatcctcg gcgacgggga cttttttcac
     4021 gagctgctgg aaggcctgga gttgcgcgcc ggtgaagagt cgctgcaccg gctcgccctt
     4081 cactccggtg gtgcgggtga ccacggccac ctcctcgtcc tcacccaggt agtctatgac
     4141 gatgttgagc atgaagcggt cgagctgggc ctcgggcaac gggtaggtgc cctccatctc
     4201 gatggggttc tgcgtggcga gcacgaagaa gggctcgggc aggcgcaggg tgttcccggc
     4261 cacagtgacc tggtgctcct gcatggcctc aagcaacgcg gcctgggtct tcggcggggt
     4321 gcggttgatc tcgtcggcga ggatcatgct ggcaaagaca gggcctttgt tgaagacgag
     4381 ctggcggccc tcggcggtgt cctccaggat ctcggtgccg gtgatgtcgg agggcatgag
     4441 atcgggcgtg aactggatgc ggtggaagct gaggtcaaaa acgtcggcca cggtcttcac
     4501 cagcagcgtc ttggccaggc cgggcgcgcc ggtgatcagg cagtggccgc ccgccagcag
     4561 ggtgatgagc atctggtcca ccacctcatg ctggccgacg atgaccttgc ccacctcctt
     4621 gaagatgcgc tcccgcgctc cgcccagcaa tccggcgacc tcggcttcgg tggctgtttc
     4681 aggaagggag gcggcgggtt cggcggggaa ggacatgacg ggttcttatc aaacagcgcc
     4741 gggcgtgcca agctatcaac ggaggcgcgc gggattcagg agggcggagc gagtctgtgg
     4801 catccaagaa aaaaagcccc ggcagcggac tgccggggct ttgttttttg tgaatcactc
     4861 ctgcgcgccg agggtgacgc cgccggcttc ggggtcgaga tcgccgacgc ggttgctttg
     4921 gaaccagccg tagccgccct ggctggcacc cttctggatc aggatgccct ggaagacggg
     4981 cagcgcgcgg gctttgtcct cccaattcgg cgtgaaggtg cctttgaacg cgccggtcgc
     5041 cggagtgaag ctcagcgtga agcttcgatt cgtcggcggc agtttggccg cggtgctgcc
     5101 ggtgatgttg aagtcggtga cgaggacatc agaggcgagc ttgccacccg caaagtggag
     5161 ctggccgttg ccagtcggcg aggcggaacc gaggccgagc gtgtcctgca cggggatcgt
     5221 ggcgtcgtag tgcgcgccga cggcgtccac ggtgatgcct tccggccaac ccccggtgta
     5281 gagctgggtg gcgcgggcgg cggcggtggt gcctgccagc tcggtgactg cgggcctgat
     5341 ccagacgagg tcctcgccat gcacgtcgct gtcctcttca tgcgtgtcga aggctaactc
     5401 gcccgcgaag gttccgccga ggtctttcgg tccgccgccg ggggtgcgca gttgggcgaa
     5461 gagcggggcg atgatgtggt cctcgtcctc atgctcgcct ggatcggcgg gcagggggtc
     5521 gccatgatca tcgtgatgat gctcaaccac cagctcggtg ctggcggtga acttggtgcc
     5581 gtcggccagg gtcgccacgg tgcgcagctt gccatcagag gacagtgtga gattggcgaa
     5641 gccgtcaccc cggggataat cactctccgg cagggactgg tctttcgagg gcagggagag
     5701 ggtgaaaagg cccggcagtt ccgcgggagc ggggctggtc ttgctgaacg cggcgggcag
     5761 cagggtgccc gcgacgtgga tttcctcgtc ttcaggatca ctggcgatgt cgccatgatg
     5821 atgatcatca tggtcgccgc cgatgtggac gtggaattca ccgtcatgga agtccatcgt
     5881 catgtggcgt ccgtcgaatt cgaactctgg ttcgagttcg ccgtcatgat tgaacatcac
     5941 cgcgccgctg gcataaacca ccgccacgaa agagcgggtc tcgccgccga tgaagacctt
     6001 gccggagaat gcgccggtgt tggtgacgac ggtgccggtg acctggccga cactgtcgag
     6061 cgtggtggca tcctcctcat gctcgggatg cagcaggccg aggaagctgg cgcgcgtgcc
     6121 gaggccaaag aaggggtttt cggcgaacac ggcccgaatg cgcgggacat tggcggcggg
     6181 catgacaaag gcgatggtcg cgccttggat attggctccc gcaggcacac cctcccagtg
     6241 gctgaaaaga tagccagact tggctttggc cgccagagcg acaatcgtgc ccggaagcac
     6301 ctgggagctt tgcagatgac tgtcacgcgg agtcagcgcg gtgaaggcag tgcggggaag
     6361 ggtgacgagc atgagggtgc cggcttcatc accaggatca aggtggccac ttccgtcacc
     6421 atgaccgtgg ccatcatcgc catcatgggc gtggtcgtca tcatcgtgat gaagctcaaa
     6481 acgccccacc gccagcagcc agcgccggct gaaggtgaag ctgcgcgtgg cggtggtgct
     6541 ggcgttgccg ctcatgtcga aggaggtcac ttcgagcgtg ttcgcgcctt cggcagggat
     6601 gatgtcagca tgccaataga ctgcgttcgg acgtgaatcc tcgctcagct cagtttcgat
     6661 gggctcgccg ccgttgagca cgacctcgac gcgcgagacg ccgtttttgt ctcccgccgt
     6721 gccgcgcgtt tcaaccggcg cggccaggct caggctggcg ttggccgccg gcgcggcgat
     6781 gcgcacggtg gggttcatgg agtcgttcgc gagcacgcgc acttcggcgc tggagaggcc
     6841 gaggctgaca tcgttggtgg ggttgctcag ggtgacgttg aagcgggtgt tcggcacatt
     6901 gcccgtgcgc ggcagcagcg cgatgtccac cgtcttactc gtctctccct cggcgaagtc
     6961 caccgtggtg gcgggagcgg tcagcgcggt gaagtccgtg ccagcgacac cggcggcgaa
     7021 gggcgggttg gtcgtctggg cggtgccgtt gctggtattc acagtcaccg aggtggctgt
     7081 gacggcaccg gtgcggttca gcgtgacggt ggcgagcgtg gatccttgat tgtaggtgta
     7141 agccgcctga ctcagggcga tgcgaccggc ggtttcctcc gccacttcga agtagaaggt
     7201 gacttcgtcc gactccactt cgccatcgtc gtgatgctcg cccgaagctt tcaacgtcac
     7261 agcccacaga ccgggctcgc tgaaggccac gaaagagtga tcgtgagtgc cggcgggcat
     7321 ttcgaaatga tcgtcctcgg tgatgccgcc gagcgcggtg gacatgacga agtcgaagcc
     7381 gccaaggccg tcagattgat acacggagaa gtgcccggtg ccgctcggcg agctgacgct
     7441 gaccagggag aatgtcagat cgctttccca ttcagcgggc tccagctcct cggtgccaaa
     7501 gcccaggaag ggcagctcgg cattgggcgc ggtgggcaga atccacaccg tgccaccctc
     7561 gctgacacct gctccgctgg cgatggccgc ggtggccacc tgctgcgccg tttcggagac
     7621 gacgatgacg gtttgatccg ctggcggctc gctgtccgcc tcctgggccg cgccgttcac
     7681 caccgcgccc tcatgcaggt gcagatgcag ttccaactca ccgccctcct cataggccag
     7741 accgatgtcg ccatgacccg ccgtccagcg gaaggggccg acttggaata ggaaggtcac
     7801 ttccacagac tccacaacgc cttccgcgcc atgctcgcct tcagcctgga cggtgacttc
     7861 ccagagaccc ggctcgctga aggcgatgaa aaagtggtcg tgcgtacccg gactgaggct
     7921 gattcgatca tcctctgtca atccgccgag agcggtggaa acaaggaagt caaaaccgcc
     7981 gagaccatca gactgataga ccgagaaatg cccgctgccg ctgggtgaat ggacatcttt
     8041 gactttgaaa tggatgttac tgttccattc agcagggtcc agctcctcgg tggcgaagcc
     8101 cagaaacggc agggcgggat tattcgaggc gggtagaacc caaaatggaa cgccttccgc
     8161 cacgccagtg cccgcggaaa tggcggccgt ggcagtctgc ttggcggcgt ctgaggcgac
     8221 gatgagcgtt tcgtcagggt cggcttcgat gccagcagcg agcaccgcgc cattgaccac
     8281 ggagcccgca tcaaggtgca tgtgcagttc aagctcccca tcgtgccaat gcacaccgat
     8341 gtctccgtga ccagcggtgt aggaggagag gccgtgggag tggccttcat gggcggggag
     8401 cgcctgcgcg gagagcagca ggccgagagc ggcaaggatg cgtgggattt tcatggcggg
     8461 gcgcacatga agtctgatgc aactcttttg caacagcaaa aacgcaaata tattgcgtta
     8521 gaaacccacc cttccggggt cagtcgggct tgaagacgaa tcgaaaaaag cgcctctccc
     8581 ccggtgcgtc gagcacgcgc agtcgccgca gcatgacgcg cccgtaggca ttgcccgcct
     8641 ccgcgcaggt ctcgcaggtc tccagagcac ggcccgtcac acgcgcgttg cgcgtggtcc
     8701 aggtctgcat gtccggactt gtgctggggc tttgctggaa ggtcttttcg ttgagcgtgt
     8761 ctttgtaagt atcacgcaag aagtccatga cagcagcgtc gccttcaggt cggcctttcg
     8821 gcagcagacc ggcatctcct ttcaccggat cacagccatg ccaaaagagc tgaaactccg
     8881 caccgttgct caggccgtcc ttgtcgtagt ccgcgcgcgt gttggccagc gtgttcgcgg
     8941 gcaggccgtg cgtgcgctcg aagctgtcgc gccactgcgc gtaggtgtag gcggcggtga
     9001 ggttggcacc gaaggccagc accatggggc cgcttttcac cggctcaccg ccttgagttc
     9061 ctgccaattc catcgtcact cggtagagcc cgttcgccgt aaaaaccgcc agcgcctcta
     9121 ggtccgcgcc gctgctgagt gtgatctcat ccgcagcatc cacaccatcc gccgagttca
     9181 tgagcaagcc tgtgccgtcc gcgttcagca cggcgaagtg gcccggcccc gacacggaga
     9241 gcaggcgcag cttcaccgca tccccgtcca gagcaccagc aggcaggcct tgcgcggcga
     9301 gcgcgggcgc gaggccgacg aggttcgcga cggggctgcc tgcggtggaa aaagtcagcg
     9361 gcatctgacg cgcggcaccg gggagcgtgg tttgcgcttc tcccgaagcg gcggcggagg
     9421 cttcgaggaa tccctgggtg ggcgtgtagg cgacgttgtc cgccgcgtcc tccaccagca
     9481 gatggaagtt ttttcccgcc ggaacaaacc ccgcgcgcac cgtagcctgc ccttgcagcc
     9541 ggctggagaa aggcacggca aagcggaaga ccttctgtgt cgaggtgagc tgcccgccat
     9601 gctgcgggtt cagcctgccc aacgcctcaa tggtgaggaa gtagctgccc agcttggtga
     9661 agccccagtt gaaatggctg tgcgcgttcg gcggcagggt ggggatgaga ttgttcgtct
     9721 gcgggccaaa gtaaaacagc agcgtctgac cgtcactctc ccagagtcca aactggccgc
     9781 ccgcgtctgg tccagtgccc gtcacgctca ccaggcgcag ggagatgctg cccgtggcgg
     9841 agggggagta gttgccattg aagaaggtct gaaaaatacc gggggccatg acggcgcgcg
     9901 cgcccatgaa gggcgtgccg aggacattgt tttgcggcat gaaccacagc ggcgcgccca
     9961 cgggaccgag gcgggacacc gcgctggtga tcaagatggg attgccattg tcaaacatcc
    10021 ccgtgcgcgg cgaggcgatg atggtggtcg tctccgggtc cagccgcacg atctgggtgc
    10081 ggtcgttgaa gttgtcgttc ttgttgtagg agacattcaa ttgccagccc gcgtccggat
    10141 ttcccggagt cagcacatag tcggcatgga tctcgaaatg cccgtagagg atgtccatcg
    10201 gcggcgtggc ctctgctgct ggcagggcca ctccgaaacc gagaatgaaa ctggacatcc
    10261 gcaaggagag catccttgat aaatgcaaat atattgcaaa gctcaagcct tgttttcacg
    10321 cgcacgccct ctctttgctt tgctggagcc gtcatcccgc acggcccgcc ttttgctgac
    10381 cgcgtttctc gcgtgtcatg cttcttttgc gcaggcgcag acggagcctt tggacctgcc
    10441 gccgggcctc atcgagctcg acatggaaac cggcgcgccc ctggatgccg ccgccgccgt
    10501 catgccgggc gagcctgagc tgttgcagga agtcgtggtg gaggcggagc gggtcagcgc
    10561 ggcctcgggg ctcaccgtcg ggcgggccat gcgtgtggcg ggcatcgcgc tgcaaccggt
    10621 cacagagatg agcggggaaa agctggcccg ccgcgcgcag ggcacgctgg gggggacgct
    10681 cgcgtgggag ccgggcgtca cctccgcata cttcggcccc ggctccagcc ggcccatcat
    10741 tcgcggcttc gatggcttcc gggtgaaaat gctgcgcgac gatctcagca cgatggacct
    10801 ctccgacacg agcccggacc acggcatcgg cctggagccg ctgctggccg agtccctgga
    10861 ggtgcatcgc ggtccgtccg cgctgctgta tgggaactcc gccatcggcg gcgcggtgaa
    10921 cacccgcagc cgcaccctga cacgggagct gccgctttcc gcgctcacgg gcgggctcga
    10981 ctcccgcttc gagagcgtct ccagcggctg ggcgcagact ggatatttca cgctgacgca
    11041 cgcgccttgg gtgctccagc tcaccggcag cgcgcgcgag tccgaggacg tgcgcatccc
    11101 cggccgcgcg cgcacggagg cgtatgaaaa agccgagcgc ccgcgcgtct cgaaccccgg
    11161 cgcgggcacc gtgagcccca tcccgaaccc ggacggtgtc ctgcccaaca ccgcccaccg
    11221 ctcccgcagc ttttccgccg gcctttcctt cctgccggac ggccccttct gggccggcgt
    11281 ctcctggtct tatttcgaca gcttttacgg cctgccctat atcttctccg gcgacgccac
    11341 cgacttcttt ggcgactcct tcattgacct ggcgcagagc cgcttcgatc tcgagctcgg
    11401 cctcgatctc gactctggcc cgctgaaaaa aatccagctc cgcctggctc agagcgatta
    11461 cgagcacgac gagcttttcg aaggccgcgg gcgcgactcc ggcgcggagt tcatcgagac
    11521 cgccatgtca aaaaacaccg tcgaaggccg gctcgatttc ctgcacggcg gcttcggcga
    11581 gcggctggat ggcatcctcg gcgtgcatgg ctttgacgag gacttcgacg ccagccgcct
    11641 cgtcgtgccg ccgcccgcgg agttccgcgt gccctcgtat ttccagaccg aaaacctcgg
    11701 cgcctatctc ctggaaaagc tgcgcctctt cgagaactgg cagctccagc tcggcgcgcg
    11761 ctgggagcgg cagcgcatcg tggacgactc cctggccggc attgtcaaag cccgcgcgca
    11821 gtccgatgag agcctcagcc actccctcgg cctgacctgg agcacggaaa acctgcccgg
    11881 cgtgaaaaag ctcagcctca ccggcatcgc ctcgaagacc gagcgcatcc ccaccgccac
    11941 cgagcgctac gccttttgga acaacgccgg cctcggccgc ttcctcgtcg gcggtgatct
    12001 ggatggcacg ccgctcggac tggaaaaatc caccggcttc gagctcggcc tcgccgccga
    12061 gtgggagcgc gccaccctgc ggctgaacgg cttccactac gactacgaga acttcatttt
    12121 cctccaggaa atccccgtct tcatccagcg cgccgtcgag tacatcgagc gggaggccac
    12181 catccagggc ttcgaggcgg agctggatct gcgtctttgg caaagcggca gccgcgcgct
    12241 ccagctcacg ctcatgggcg actacgtgaa cggtcacaac gacaccgatg acgagcccct
    12301 gccgcgcatc ccgccgctgc gtgccggcgg ccgcatcgag ttcaccacca gccgcctcac
    12361 cctcggcctg gaggcgcgcc acagcttcgc gcaagaccgg ctgaagcccg agccgcgcgg
    12421 cgagctgccc acggagggct acaccctcgt caatgccgac gcctcatgga gactctcgaa
    12481 agggcggcat gatgtgctgc tcaccctgca agtgacgaac ctgctgaatc aagacgcgcg
    12541 ccagcacacc tccttccgca aagacgtggc tcccctgccc ggcatcggcc tcgtggccgg
    12601 ggtgcgctgg acgttttgag cctgcgcatt ccccgccgac accaaaacgc ccgttttttg
    12661 gtcctcccac gggcgtggcg acaccaaacg ctcgtttttt ggtcctccca cgggcgtggc
    12721 gacactaaaa tcctcctttt tggtactccc acgcgcaggg gcgtcaggct ttcacggctt
    12781 cgggctcgac ggcgacgggc tcctccgggg gcacctgcac gggctcggcc ttgctggtga
    12841 ctttgacgaa cttcggcagc caggtgtccc aggcatcgag gatgtctttg gcgcgctggc
    12901 tttcggtgaa ttcgaggtgg tcggtgatga gcttgcggag ctggttgatg tcctcgggat
    12961 cggtgacggg ctcgcctttg atcatctccg ggttgtggag ctggtggaag gcgccgctct
    13021 cgtcgagcag gtaagccacg ccgccgctca tgccggcgcc gaagtttttg ccgaagctgc
    13081 cgagcacgac ggccatgccg ccggtcatgt attcgcagcc gtggtcgccg atgccttcga
    13141 cgacggcggt ggcgccggag ttgcgcacgc agaagcgctc gccggcgcat ccggcggcgt
    13201 gcaggcggcc gctggtggcg ccgtacatga cggtgttgcc gaggatgctg ttttcccagg
    13261 atttgaagct ggggtgggct ttggggctgg gcttgatgat gatctcgccg ccgcagaggc
    13321 ccttgccgac gtagtcgttg gcctcgccga cgagggtgag cttgatgccg ccgcaaagga
    13381 aggtgccgaa gctctggccg gcgctgccct cgcaggtgac gtccacgctg ccggggggca
    13441 gtccgtggtt gccgtggtgg aaggcgatct cgccgctgag tttggtgccg atgttgcgga
    13501 aggtgttttt gatcttgtag cgcacgggcg tgaccttcgc cttgtcgcgg atggcgaact
    13561 gggcgttctg gatgatgcgg tcgtcgagcg ggtgggcgtc gaggccgtca ttctggttca
    13621 tgaggcagat gcgcggggcg tcctggccga ggtccttgcc cacgtccttg agcacgcggc
    13681 tgagatcaat cttgctggct tttttgtggc cgggcacctg gcgctggcgc aggaactcgg
    13741 ggcggccgat gaggtcgtcc attttggcga ctccgaggct ggccatgatc ccgcgcacct
    13801 cgtgggcgac gccgttgaag aagttgacga cgtgctcggg cttgcccttg aatttggagc
    13861 ggaatttggg gtcggtggtg gcgacaccga cggggcagtt gttgaggtgg cactggcgca
    13921 catagacgca gccgagggcg atgagggcga tggtgccga
//
LOCUS       DAOMYJ010000132        13946 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Prosthecobacter sp. isolate AMR_MDS_5629
            NODE_1462_length_13946_cov_19.332589, whole genome shotgun
            sequence.
ACCESSION   DAOMYJ010000132 DAOMYJ010000000
VERSION     DAOMYJ010000132.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867850
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prosthecobacter sp. (wastewater metagenome)
  ORGANISM  Prosthecobacter sp.
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Verrucomicrobiaceae; Prosthecobacter.
REFERENCE   1  (bases 1 to 13946)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13946)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.30
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:34:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,742
            CDSs (total)                      :: 4,684
            Genes (coding)                    :: 4,676
            CDSs (with protein)               :: 4,676
            Genes (RNA)                       :: 58
            tRNAs                             :: 55
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13946
                     /organism="Prosthecobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1462_length_13946_cov_19.332589"
                     /isolate="AMR_MDS_5629"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1965333"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            190..1278
                     /gene="pheA"
                     /locus_tag="PLP58_13780"
     CDS             190..1278
                     /gene="pheA"
                     /locus_tag="PLP58_13780"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: protein motif:HMM:NF012998.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate mutase"
                     /protein_id="HRJ09643.1"
                     /translation="MSLEEARQKIDAIDQQLIHLLNERAEMVHKIGEIKKKDGLDIYV
                     PGREEKLLRKLCELNTAQNGKLTEKAIRAIFREIMSAALALENSLKIAYLGPAGSWTH
                     QVAINKFGNSVSYVAEASTEGVFARVAAQETDYGVLPIEHSTEGAVHHTLDRLVDSPL
                     QIYAEILWKVETVVMSKTDAAQVTVIFGHPQMLAQCRPWLARCFPAARLVESASASLA
                     ATHAETEPGAAALGTPLGAELHALKVLAASPGEHTSRARFIILGRRSGAPTGHDNTML
                     MVHARDRVGALLEVLQIFARRNVNVRQIENRPVPGDCEGVQARFFLEVSGHHEEAPIQ
                     ESLADLAALGSTVKVLGSYPAPGWIEER"
     gene            1325..2017
                     /locus_tag="PLP58_13785"
     CDS             1325..2017
                     /locus_tag="PLP58_13785"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009966067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HRJ09644.1"
                     /translation="MKKNKLVVITGCTRGLGRAMVGEFIRHGWTVAGCARTAEAVQKL
                     QAAHPAPHFFSVCDVADEARVAGFCKEALAECGVPDLLLNNAAVVNTSAPLWEVGAEE
                     FGRVVDVNIKGTASMMRHFMPAMLARGSGVVVNFSSGWGRSTSPEVAPYCATKHAIEG
                     LSMAVAQETGGRVAVIPLNPGIIDTEMLRSCFGDGAAHYPGPEKWAVKAVPFLIRLGP
                     GDNGRPLTAPGG"
     gene            2154..2765
                     /locus_tag="PLP58_13790"
     CDS             2154..2765
                     /locus_tag="PLP58_13790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009966066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HRJ09645.1"
                     /translation="MPASPAPEPAEIPDTELVKRSQAGDSRAFDLLVTRYRGRIYAMT
                     YHMIQNETEAWDLAQEAFIKAWRALPGFKMDSAFYTWLYRIAHNVTYDWLRKKKIQGD
                     GEFDDSRTEHKIAAGAETAPYADAAPDEALKNAELGEKIRAAIAQLSADHRQVIIMRE
                     IEGMAYDEIAAAIPCSIGTVMSRLFYARKKLQELLKDTYENAK"
     gene            2749..3375
                     /locus_tag="PLP58_13795"
     CDS             2749..3375
                     /locus_tag="PLP58_13795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ09646.1"
                     /translation="MKTPSEDHDLIRWLDDELPPLERERFVTRMNADPILRGEAEAMR
                     ELARELRAHLPKTLPVPHADFFNSQIQVRIAREENPSRASRTPAGSWLEWFRLPGLAL
                     GAAAAALVFGATFAWQHESLKNSLVLTTYAPDPGVTAQTWFSDDAQATVLMLDGLEEM
                     PADRPLVGFLREANAERQMLAVALQNEHGTPHARPRPDAVPLTANLGE"
     gene            3372..3857
                     /locus_tag="PLP58_13800"
     CDS             3372..3857
                     /locus_tag="PLP58_13800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ09647.1"
                     /translation="MRILAFICCLCAMTHPGQGIAARPDRGQPAIEGKVWGALIHATA
                     ASSDAAKTSATAPVIRDLQQRLAKVFPEHGRFEIIGEHLQDVFREYESWVVPSKDIFL
                     KVDSKGPAPGGGINLHLQFWRDQQVLVKTDTILKPGSPLFIGGPKWREGRLIFVLVLQ
                     E"
     gene            complement(3903..4841)
                     /locus_tag="PLP58_13805"
     CDS             complement(3903..4841)
                     /locus_tag="PLP58_13805"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HRJ09648.1"
                     /translation="MSVTIAFTSLSPADHEAVARLLHRSLVDWYESRLRQGARFGESH
                     EPFLLFPQVYESLDAGECVTARDEATGEMLGVCFSHERETHVAVGIVATAPEAAGRGI
                     ARGMMQAVLEKARRLGKPARLVSSLLNLDSFSLYTRLGFVPGVIFQDMLITVPDTGMA
                     VPAPQGVEQVREARTDEAGRIADFEEALQGIRRDKDIAFFLRNEVGSWRVLVKEGADG
                     ELSGFLALSTHPSFTMIGPGVCADEESAAALLWSALDGLRGRTLVFLVPCAAAGLVRM
                     AYGWGARNVELHVAQSTAPVAGARGIVFPTFMPESA"
     gene            complement(4862..6268)
                     /locus_tag="PLP58_13810"
     CDS             complement(4862..6268)
                     /locus_tag="PLP58_13810"
                     /inference="COORDINATES: protein motif:HMM:NF013567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HRJ09649.1"
                     /translation="MSEPSSSRRHFLKTAAAATAVSTFNIVPRHVLGGPRFVPPSEKV
                     NVAIVGAGGQGRVNLRQLLQLDDVQVIAVADAAESFSLENFYYKGMGGRLPTKADIEK
                     HYAEKTPNFRCADYEDFRVMLKKEKAVDAVLCATPDHLHAYVSCVAMRAGKHVYCEKP
                     LTHNIWESRHVAKVAAETGVATLLGSQGHSSLGTRETIEYIQDGAIGTVREIHSWVGA
                     KRWNPTLTGKPADTPPVPAGLNWDLWLGPREERPFHPAYFPVAWRDFWAFGTTGIGDF
                     GCHDLNTAVRAFDLPLPERVQAFAAGVMDSEIAPHGSIIHYLFPAGEKRPPMKLTWYD
                     GGLMPEKPEALGAFPLPRRGTLYVGDKGVIQCDGSGGAPRLFPDVLRASYQKPEPRLK
                     RSAGHHRDWIDACKGGPAASSPFDYSAHLTEIVLLGVLSLRTGKEIVWDAANMKATGL
                     SAADAFIRESYRPGWEVV"
     gene            6267..8342
                     /locus_tag="PLP58_13815"
     CDS             6267..8342
                     /locus_tag="PLP58_13815"
                     /inference="COORDINATES: protein motif:HMM:NF027452.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4838 domain-containing protein"
                     /protein_id="HRJ09650.1"
                     /translation="MAGEYGARQSRLSVKWRAKPLSGKNPARSAFLRLMPSRVFILLA
                     GAGLSIHAADLVLVDKDTPPAPIVIFKDAPPRTREAAEVLADYIAKISGQKPPVIEGA
                     PEPLPERAIWVGAQPAVKTLFSQTDFDFKHPEEILIAANEKHVVIAGRDRWDPERLDI
                     KTKEGVIKGVQQEYGTINAVYTFLQDQLGVRWFWPGELGEDVPKSERVSISAPFEYRH
                     HPQMRARGTVFNYCRLGNKGYGKSQEWTMRQRLQLCSLSLEGGHGFGDWWERYHQKYP
                     VIFALQPDGTRSGFPNPHNAKLCMSNPKVWELWLEGVEEQLRQDPHRTIFNASPNDGW
                     ASGHCVCDNCTAWDHPDGEPRVFNWFKRNETRPATSDRDVTFANTLGGLLKQKYPGKD
                     YRVMMLSYGHSRPAPVKARPADNVIMTVVANFFGRTGLVDRGSTRGDTYRQQFEAWAK
                     IVPSMFWRPNTGSPAGWQQGLPDIHVAQTIRDFKDVAAANCIGIYIDGVWEHWSTVGP
                     LYYIMAQLAWNPAADADAILADYYTRAFGPAAPQVREYFEALEKERMAFTAKNGEAGV
                     FTFPQLYTADLLSESQARLDRAAAAVPADSIHARRVAFIQAGLTYTRMMVENIVLMDR
                     YWKKKDAAIAAQVRKNWAVIEEHVAAHPYAINWGPVRPTTPRMAGLHPDSAPPKKPKQ
                     VRVNDLDLN"
     gene            8355..9788
                     /locus_tag="PLP58_13820"
     CDS             8355..9788
                     /locus_tag="PLP58_13820"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase"
                     /protein_id="HRJ09651.1"
                     /translation="MIQALLTSLTCLTLAYGSAAASKPNIVLIFADDLGWQEPGFAGS
                     DFLETPRLDQLAREGMVFRHAYASAGNCQPSRACMLSGQYTPRHGVYAVGSTDRGPKT
                     LMRMIPVPNRPNLPPDTMMLGESLKAAGYATGFFGKCHMKDSKTGKSEHAGFDVIQHS
                     KPGLNSDDPADPKAIFSITNAACEFIEANRERPFFAYIPHYAVHSRLEGRAETLARFK
                     NKAPGKLGHDHAMLAACLSDLDTGIGMVLDKLKAIGLEENTLVVLTSDNGGTHVSNEP
                     LRGKKGGYYEGGIRVPMIARWPGVTTPGSTCDTPVSNVDFYPTFLAAAGASAPASPLD
                     GESLLPLFKGQGALQRQALFWHFPGYLDDPVPRGRDPNFRTRPVTAMRKGEWKLHLYH
                     EEWQLDGGREKLATNRAAELYHLAADPGEHTDLAATDTAKRDELLEDLLAWMKSTSAP
                     LPQQTNPEYNSKSSGKGGKKKKQAGKD"
     gene            10078..10614
                     /locus_tag="PLP58_13825"
     CDS             10078..10614
                     /locus_tag="PLP58_13825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018970140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5069 domain-containing protein"
                     /protein_id="HRJ09652.1"
                     /translation="MKHYIWDSYFVELFDACVDEYDEGNRDFEAWFSDEDQEFLKSIG
                     CREREFFDFVEDNVTSGGEDPTAITALLVAAVRRDFFLAIQKGVPSACIVQPSELPAK
                     TAEMDGIPWLPRIIAKARAKLRGEMDPDTMFGCGGDRAFLSKFDIHPADFLRVVWAAG
                     DEDEKILRHVRERQAAIA"
     gene            complement(10621..11334)
                     /locus_tag="PLP58_13830"
     CDS             complement(10621..11334)
                     /locus_tag="PLP58_13830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ09653.1"
                     /translation="MKSRLPHVILAAVLCSGAARSVEPPRDGIHDDARAFGAQTRQVL
                     AWEIAGASARAGCAFWVSVDSYNGSGRNIRRHARDLRRAWSGDADAVLLAYERSSNQL
                     GFSFSPGLWEKHTPAALAVLVHEAGASMSRTDTSLEERLSGAVSVLLEGMRRLEEGRI
                     RSARPLSIADRRLAAYFTPVLAAGALLFFAAGMIARRGPGMDTRESCFPEVQVGCRFG
                     APHGGGVVAVAAPRNGTHD"
     gene            complement(11382..13478)
                     /locus_tag="PLP58_13835"
     CDS             complement(11382..13478)
                     /locus_tag="PLP58_13835"
                     /inference="COORDINATES: protein motif:HMM:NF013283.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BamA/TamA family outer membrane protein"
                     /protein_id="HRJ09654.1"
                     /translation="MVTRSQSGKLIAALHGCLLLWLGIAPAMRGAEPEPAGEPFRLRE
                     GSVSLRVHGIPGTAWEGLEQMLKDQLTLHGGGITAPLADDLAFFTRQHLLRAGWPEAE
                     VRWNIENDRIHLRAETGRPARVGALTWTGGRVLPEDEMRRFLLRPALEKEGADKRHPL
                     WVDSDLNSGAALLTRRLRSEGYLLAEAALTPASEPGADGLRALAIEVKPGPQFVFGGV
                     ALDGAPDELSGEMSREIAQTPGQPFNEAAVQALERRLNHIAVSRGWLSSVTSSEYHLG
                     SQGGTVDVTLRMAPGARARVARALPHEGFSRGARRVLEAGFREAEGEWYSSKDTDFLF
                     RRALDTGMFARLDVEPVIQPGNPEQPALADLVISGEETKPRTLGFEAGYDTFLGPQAG
                     VNYRNTNLRDTGVTLATELNWSAAGPLGFVSLTDPAVFNSSHSGSVRLAVETFGLYEY
                     TRYGSSLHAELARRLTPFFSYTAFFGASVNSISTDTLSSEETGPGTYTLATLGGSMML
                     DFRDSPVLPTKGWFLSARLETTLDVMGSSVGFLRTDLRGAWHLPVTKKSRLTLGGALM
                     SIQGAAAEELPIDARVFNGGPNSVRAFAERELGPLTTGGTPLGGTSALLGTLEFSHEI
                     YPNLEFAVFGDIGSLGRGSNTSALDISTDFRQAAGAGLRYKLPFGPVRVDYGHNTSRR
                     SGEKSGMLHVTVGFSF"
     gene            complement(13584..>13946)
                     /locus_tag="PLP58_13840"
     CDS             complement(13584..>13946)
                     /locus_tag="PLP58_13840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018970155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S24 family peptidase"
                     /protein_id="HRJ09655.1"
                     /translation="IAAGYAENQEQQADGSISVDVNTLGLARGSRVFALKVRGESMIG
                     ASICDGDVVILEAREPRPRDIVAALIDGETTLKRYLVSDGKPYLKAENPAFPDLIPVR
                     NLEVQGVMQTLIRQVRRD"
BASE COUNT         2606 a         4697 c         4348 g         2295 t
ORIGIN      
        1 cgcgtgaagc tgccctcgcc ggaatccggg caggcctccc cggcagcgga ggagaagcag
       61 ctttccctgg cccccgtcag tgatgacgga gaagcgaacg cccaggcccc ggctgacgag
      121 ccatccgccg ccgccgcgga tgccgcgccc tcctccggcg agtgacccct atccccaccc
      181 gcgtgactca tgtccctcga agaagcacgc cagaaaattg acgcgataga ccagcagctc
      241 atccacctgc tgaacgagcg cgccgagatg gtgcacaaga tcggcgagat caaaaaaaag
      301 gacggcctcg acatctatgt gccgggtcgc gaggaaaagc tgctgcgcaa gctttgcgag
      361 ttgaacaccg cgcaaaacgg caagctcacc gagaaggcca tccgcgccat tttccgcgag
      421 atcatgagcg ccgccctggc gctggaaaac agcctcaaga tcgcctacct cggccccgcg
      481 ggctcatgga ctcaccaggt ggcgatcaac aagtttggca acagcgtctc ctatgtggcc
      541 gaggccagca cggagggagt cttcgcccgc gtggccgcgc aggagacgga ctacggtgtg
      601 ctgcccatcg agcactccac cgagggcgcg gtgcatcaca cgcttgaccg gctggtggac
      661 tcgcccttgc agatttacgc ggagattttg tggaaggtgg agaccgtcgt catgtcgaag
      721 accgacgcgg cgcaggtcac tgtgattttc ggccatcccc agatgctggc gcagtgccgc
      781 ccctggctgg cgcgctgctt tccagccgcg cgtctcgtcg aaagcgcctc cgccagcctc
      841 gccgccactc atgcggagac ggagcctggc gccgccgcgc tcggcacgcc tctgggagcg
      901 gagctgcacg cgctcaaggt actggccgct tcgccaggag agcacacttc ccgcgcgcgc
      961 ttcatcatcc tcggccggag aagcggcgcg cccactgggc atgacaacac catgctcatg
     1021 gtccacgcgc gcgaccgtgt cggcgcgctg ctggaggtgc tgcagatctt cgcgcggcgg
     1081 aatgtgaatg tgcgccagat cgagaaccgt cccgtgcctg gcgactgcga gggcgtgcag
     1141 gcgcgctttt tcctggaggt gagcggtcat catgaggaag cgccgatcca ggaaagcctg
     1201 gccgacctcg ccgctctggg ctccacggtg aaagtgctcg gcagctaccc cgcgcctggg
     1261 tggatcgagg agcgttgagg caaagcgtga caggctccag gcttgaggcg catatattcc
     1321 gctcatgaaa aagaacaaac tcgtcgtcat caccggatgc acgcgcggtc tgggacgcgc
     1381 gatggtggga gagttcatcc gccatggctg gaccgtggcc ggctgcgcgc gcacggcgga
     1441 ggcggtgcaa aaactgcaag cagcgcatcc cgctccgcat tttttctccg tctgcgatgt
     1501 ggcggacgag gcgcgggtgg cgggcttttg caaagaagcg ctggctgagt gcggcgtccc
     1561 cgacctgctg ctgaacaacg ccgccgtggt caacaccagc gcgccactct gggaagtcgg
     1621 cgcggaggag tttggccggg tggtggatgt gaacatcaaa ggcaccgcca gcatgatgcg
     1681 ccatttcatg cccgccatgc tcgcgcgcgg gtcgggcgtg gtggtgaatt tctcctccgg
     1741 ctgggggcgc tccacctcgc cggaggtggc cccgtattgc gccaccaagc acgctattga
     1801 gggactctcc atggccgtcg cgcaggaaac cggcggcagg gtggcggtca tccccctgaa
     1861 tcccggcatc atagacacgg agatgctgcg cagttgcttc ggggacgggg cggcgcacta
     1921 tccggggcct gaaaaatggg ccgtaaaagc agtgcccttt ttgatcagac tcggccccgg
     1981 tgacaatgga cgcccgctca ctgcgccagg tggctgaata gacaccgccc gcgccacttg
     2041 gctgccgctt cggccggagg cgaaatctgc gctttgacgc tgaggtgttt tgcgtgagat
     2101 tggcctgaat aagcgttccg gcgctgccgt ctccacctgt gacatcccag aacatgcccg
     2161 cctcccctgc gcctgagccg gccgaaattc cggacaccga gctggtcaag cgcagccagg
     2221 cgggcgattc gcgggcattc gacctgctcg tgactcgtta ccgggggaga atttacgcca
     2281 tgacttatca catgatccag aatgaaaccg aggcgtggga cctcgcgcag gaggctttca
     2341 tcaaagcctg gcgcgccctg ccgggcttca agatggactc ggccttctac acctggctct
     2401 accgcatcgc gcacaatgtc acctacgact ggctgcggaa aaagaaaatc cagggcgatg
     2461 gcgaattcga cgactcccgc accgagcaca aaatcgccgc gggagccgag acggcccctt
     2521 atgccgacgc cgcaccggac gaggcgctca agaatgccga gctcggcgaa aaaatccgcg
     2581 ccgccatcgc ccagctcagt gccgaccacc ggcaggtcat catcatgcgc gagatcgagg
     2641 gcatggctta tgatgagatc gccgccgcca ttccatgctc gatcggcacc gtcatgagcc
     2701 gcctttttta cgcccgcaag aaactgcaag aactcctcaa ggacacctat gaaaacgcca
     2761 agtgaagacc atgatctcat ccgctggctg gatgacgagc tgccgccgct ggagcgcgag
     2821 cgcttcgtca cccgcatgaa cgccgacccc atcctgcgcg gcgaggccga ggccatgcga
     2881 gagctggcgc gcgaactgcg cgcgcacctg cccaaaaccc tgcctgtgcc gcatgccgac
     2941 ttcttcaaca gccagatcca ggtccgcatc gcgcgggagg aaaacccctc ccgcgcctcc
     3001 cggacgccgg ccggctcatg gctggaatgg ttccgcctgc ccggcctcgc tctcggcgcc
     3061 gccgcggcag ctctcgtttt cggcgccacc tttgcatggc agcatgagtc attgaagaac
     3121 agcctcgtgc tcaccactta cgcgcctgat cccggcgtca ctgcccagac atggttctct
     3181 gatgacgccc aggccaccgt gctcatgctc gacggactcg aggaaatgcc cgccgatcgt
     3241 ccgctcgtgg gcttccttcg cgaagcgaac gcggagcgtc agatgctggc cgtggccctg
     3301 caaaacgagc acggcactcc gcatgccagg ccgaggccgg acgcagtccc gctcaccgca
     3361 aaccttggcg aatgagaatc ctggctttca tctgctgcct gtgcgccatg acccatccgg
     3421 ggcagggtat cgcggcccgt ccagaccgcg gccagccggc cattgagggg aaagtctggg
     3481 gtgcgctcat ccacgccacc gccgcatcgt ctgacgccgc aaaaacctcc gccaccgcgc
     3541 cggtcatacg cgacttgcag cagcgcctag ccaaggtctt ccccgagcat ggccgcttcg
     3601 aaatcatcgg cgagcacctg caggatgtct tccgcgagta tgaatcctgg gttgtgccct
     3661 cgaaagacat ctttctcaag gtggactcca aaggccccgc ccccggcggc ggcatcaatc
     3721 tccacctcca attctggcgc gaccagcagg tgctcgtcaa aaccgacacc atcctcaaac
     3781 ccggcagccc cctcttcatc ggcggcccca agtggcgcga aggccgcctc atcttcgtgc
     3841 tcgtgcttca agagtgatcg ttgccaccac tctccagatg aagaaaacgg cggcaaaaaa
     3901 gcctacgccg actcaggcat gaaggtggga aagacaatgc cgcgcgcgcc cgccacaggc
     3961 gcggtggact gtgccacatg cagctccaca ttgcgcgcgc cccagccgta ggccatgcgc
     4021 acaagaccag ccgccgcgca tggcacgagg aaaacgagcg tgcgtccgcg cagcccgtcc
     4081 agcgcgctcc agagcagggc cgccgcgctt tcctcatccg cgcacactcc ggggccgatc
     4141 atcgtgaacg agggatgcgt gctcagcgcc agaaagccgc tcaattcccc gtccgctccc
     4201 tccttcacca gcacccgcca cgagcccacc tcgttgcgca ggaagaaggc gatgtccttg
     4261 tcgcgccgga tgccttgcag cgcttcctca aagtccgcga tgcgccccgc ctcgtccgtg
     4321 cgcgcctcgc gcacctgctc cacaccttga ggcgcgggca ctgccatgcc ggtgtccggc
     4381 acggtgatga gcatgtcttg aaaaatgacg ccagggacaa agccaagccg ggtataaagc
     4441 gagaaggagt ccagattcag caggctggaa acaagacgcg ccggcttgcc gagtcggcgc
     4501 gctttttcca gcaccgcctg catcatgccg cgcgcgatcc cgcgccccgc cgcctcgggc
     4561 gcggtggcca cgatgcccac agccacatgc gtctcgcgct catgggagaa gcacacgccg
     4621 agcatctcgc cagtggcctc atcccgcgcg gtgacgcatt caccggcatc cagcgactca
     4681 tacacctgcg gaaaaagcag gaagggctcg tggctctcac caaagcgcgc cccctggcgc
     4741 agacggctct cataccagtc caccagggag cggtgcagca gccgtgccac ggcctcgtgg
     4801 tcggcggggg aaagggaagt gaaggcgatg gtgacggaca tgggcttggg gtgcgggtgg
     4861 ctcagacgac ttcccaaccc ggacgatagc tctcgcgaat gaacgcatcc gcagcggaca
     4921 gcccggtggc tttcatgttc gcggcatccc agacgatttc cttgcccgtg cgcagggaga
     4981 gcacgccgag cagcacgatc tcggtgaggt gcgcggaata atcaaacggg ctgctcgccg
     5041 cagggccgcc tttgcaggcg tcaatccagt cgcgatgatg cccggcggag cgcttcaagc
     5101 ggggctcggg tttttgatac gaggcgcgca gcacgtccgg gaacagacgc ggcgctcctc
     5161 cgctgccatc gcattggatc acgcccttgt caccgacata caaggtcccg cgcctcggca
     5221 aaggaaaggc accgagcgcc tccggcttct ccggcatgag gccgccgtca taccaggtga
     5281 gcttcatcgg cgggcgcttt tctccagccg ggaagaggta atgaatgatc gagccatgcg
     5341 gggcgatctc gctgtccatc accccggcgg caaaggcctg cacgcgctcc ggcagcggca
     5401 ggtcaaaggc gcgcaccgcc gtgttcaagt catggcagcc gaagtcgcct atgcccgtgg
     5461 tgccaaaggc ccaaaagtca cgccatgcca ccgggaagta ggccggatgg aacggacgct
     5521 cctctcgcgg tccgagccag agatcccagt tcagcccggc gggcacgggc ggtgtgtcgg
     5581 ccggcttgcc agtcagcgtg ggattccacc gcttcgcccc gacccaggag tgaatctccc
     5641 gcactgtgcc gatggctcca tcctggatgt attcgatcgt ctcccgcgtg cccagcgagg
     5701 agtgcccctg gctgcccagc agagtggcca cgcctgtctc ggcggccacc ttcgccacat
     5761 ggcgggactc ccagatgttg tgcgtgagcg gtttctcgca gtacacatgc ttgcccgcgc
     5821 gcatcgccac gcaggagaca taagcgtgca gatggtcggg tgtggcgcac agcacggcgt
     5881 ccaccgcctt ctcctttttc agcatcacac ggaagtcctc ataatccgcg cagcggaagt
     5941 tcggcgtctt ttccgcatag tgcttctcga tgtccgcctt cgtcggcaga cgcccgccca
     6001 tgcccttgta ataaaagttt tccaaactga acgactccgc cgcgtccgcc acggcgatca
     6061 cctgcacatc atcaagctgg aggagctggc gcaggttcac cctgccctgc ccgcccgcgc
     6121 ccacgatggc cacattcacc ttctcgctag gtggcacaaa gcgcggcccg cccagcacat
     6181 gacgcggcac aatgttgaag gtggacaccg ccgtggcggc tgccgcggtt ttgaggaaat
     6241 gacgacgact ggaggatggc tcggacatgg ccggagaata cggcgcgagg caaagccgtt
     6301 tgtcggtgaa gtggcgggcg aagccgctgt cgggcaagaa tcccgcgcgt tccgcgttcc
     6361 tgcgcctcat gccatctcgt gtctttatat tgcttgcagg ggccggcctc tcaatccacg
     6421 ccgccgatct tgtccttgtg gacaaagaca ctccgcccgc tcccatcgtg attttcaagg
     6481 acgcgccacc gcgcacgcgg gaggctgcgg aagttctcgc tgactatatc gcgaagatca
     6541 gcggccaaaa gccgccagtg atcgaaggcg cgcccgagcc gctaccggag cgtgccatct
     6601 gggtcggtgc gcaacccgcc gtgaagacgt tgttttcaca gacggacttc gatttcaaac
     6661 accccgagga gatcctcatc gcggcgaacg aaaagcatgt cgtcatcgcc gggcgcgacc
     6721 gctgggatcc ggagaggctg gacatcaaaa ccaaagaggg cgtcatcaag ggcgtgcagc
     6781 aggagtatgg cacgatcaac gcggtgtaca ccttcctgca ggaccaactc ggcgtgcgct
     6841 ggttctggcc gggggaactg ggcgaggatg tgccaaaaag cgagcgtgtc tcgatcagcg
     6901 cgccgtttga gtatcggcat catccgcaga tgcgcgcgcg cggcacggtc ttcaattact
     6961 gccgcctcgg caacaaaggc tacggcaagt cgcaggagtg gacgatgcgc cagcgattgc
     7021 agctttgctc gctctccctt gaaggcggtc acggctttgg cgactggtgg gagcggtatc
     7081 accagaaata tccggtaatc ttcgcgctcc agcctgacgg cacgcgcagc ggcttcccga
     7141 atccgcacaa tgccaagctc tgcatgtcga accccaaggt gtgggaactg tggctcgaag
     7201 gcgtcgagga acaactccgg caggacccgc accgcaccat cttcaacgcc tcgccgaacg
     7261 acggctgggc cagcggccat tgtgtgtgcg acaactgcac cgcctgggat caccccgatg
     7321 gcgagccgcg cgtcttcaac tggttcaagc gaaacgaaac gcgccccgcc accagcgacc
     7381 gcgatgtcac cttcgccaac acgctcggcg ggttgttgaa gcagaagtat cccggcaaag
     7441 actatcgcgt catgatgctc agctacggcc actcccgccc cgcgccggtg aaggcgcgtc
     7501 cggcggacaa tgtcatcatg accgtggtag cgaacttctt cggccgcacc ggcctcgtgg
     7561 atcgcggctc gacgcgtggc gacacctacc ggcagcagtt tgaggcatgg gcaaaaatcg
     7621 tgccctccat gttctggagg cccaacaccg gcagccccgc cggctggcag cagggactgc
     7681 cggacatcca tgtggcgcag acgatccgcg atttcaaaga tgtggccgcc gcgaactgca
     7741 tcggcatcta cattgacggc gtgtgggagc actggagcac cgtcggcccg ctctactaca
     7801 tcatggcgca gctcgcatgg aatcccgccg ccgatgcgga cgccatcctt gccgactact
     7861 acacccgcgc cttcggcccc gccgcgccgc aggtgcgcga gtacttcgag gctctcgaaa
     7921 aagagcgcat ggccttcact gccaagaacg gcgaggccgg cgtcttcacc tttccccagc
     7981 tctacaccgc cgatttgctg agcgaatccc aggcgagact cgaccgcgcc gctgccgccg
     8041 tgcctgccga ctcgattcac gcccggcgcg tcgccttcat ccaggccggt ctcacttaca
     8101 cccgcatgat ggtggaaaac atcgtcctca tggaccgcta ctggaaaaag aaagatgccg
     8161 ccattgccgc gcaggtgagg aaaaactggg ccgtcatcga agaacacgtc gccgcgcacc
     8221 cttacgccat caactggggc cccgtccgcc ccaccactcc tcgcatggcc ggcctccacc
     8281 cggacagcgc tccgccaaag aagcccaagc aagtccgggt gaatgatctc gaccttaact
     8341 aacctcaacc tctcatgatc caagcattgc tgacctcctt aacctgcctc acgcttgcat
     8401 acggctcagc ggccgcgtca aagccgaaca tcgtgctaat cttcgccgat gacctcggct
     8461 ggcaggagcc gggatttgcc ggatcggatt tcctggagac tccgcgtctc gaccagctcg
     8521 cgcgggaagg catggtgttc cgtcatgcct acgcgtcggc gggaaactgc cagccgagcc
     8581 gcgcctgcat gctctccggc caatacacgc cgcgtcacgg cgtgtatgcg gtgggttcca
     8641 cagatcgcgg ccccaagacg ctcatgcgca tgatcccggt gcccaaccgc ccaaacctgc
     8701 cgccggatac gatgatgctg ggcgaatccc tgaaggccgc cggctacgcc acgggcttct
     8761 ttggcaaatg ccacatgaag gactcaaaga ccggcaagtc cgagcacgcg ggttttgatg
     8821 tgatccagca ctccaagccc ggcctcaaca gcgacgatcc cgccgacccc aaggccatct
     8881 tttccatcac caacgcggcg tgtgagttca tcgaggcaaa ccgcgagcgg ccctttttcg
     8941 cctacatccc gcactacgcc gtgcattcgc gtctcgaagg ccgggcggag acgctggcgc
     9001 gcttcaaaaa caaagcaccc ggcaagctcg gccacgatca cgccatgctc gccgcctgtc
     9061 tctccgatct cgacaccggc atcggcatgg tgctcgacaa actcaaggca atcgggctgg
     9121 aggagaacac actcgtcgtc ctcacctccg acaacggcgg cacgcatgtc agcaacgagc
     9181 ccctgcgagg caaaaaaggc ggctactacg agggcggcat ccgcgtgccg atgatcgccc
     9241 gctggcccgg cgtcacgaca cccggcagca cctgcgacac acccgtctcc aacgtggact
     9301 tttaccccac cttcctcgcc gcagccggag catccgcccc cgcctcaccg ctggatggag
     9361 agagcctgct gcccttgttc aaaggccagg gcgcgctgca aaggcaggcg cttttctggc
     9421 acttcccagg ttatcttgac gaccccgtgc cacgcggacg cgaccccaac ttccgcacgc
     9481 gtcccgtgac ggccatgcgc aagggcgagt ggaagctgca cctttaccat gaggaatggc
     9541 agctcgacgg cgggcgtgaa aagctcgcca ccaaccgcgc cgcagagctt tatcatctcg
     9601 ccgccgaccc cggcgagcat accgacctcg ccgccaccga caccgcgaaa cgcgacgaac
     9661 tgctcgaaga cctgctcgcc tggatgaaat ccaccagcgc tccgctgccg cagcagacaa
     9721 acccggaata caattccaag tcatcaggca aaggcgggaa gaagaaaaag caggccggca
     9781 aggattgatc tcaaaaacag ccaccgcttt gcctcttttg cggattttac ttttcaattc
     9841 tccagtctga ttgcggagtc atccgtcctt cggttttgtg tttgggatct gccgaacact
     9901 tttctctttc gcaccccccg gagcctggga cctcaatgcc tgatgcatga gggcggaaca
     9961 gcgtcgcggc gtcagctcgg cacgactccg acaccatgcg caacggttca ggccggcctg
    10021 gaaaaaagcg ggaatttccg cgtgccatcg ccctgcggcg cgccaaagat gggcgcgatg
    10081 aagcactaca tttgggactc ctacttcgtg gaactctttg acgcctgcgt ggacgagtat
    10141 gacgagggca accgcgattt cgaagcctgg ttcagcgacg aggaccagga gtttttgaaa
    10201 tccatcggct gtcgtgagcg cgagtttttc gactttgtgg aggacaatgt gacctccggc
    10261 ggcgaggacc cgacggccat caccgcgctg ctggtggcgg cggtgaggag ggactttttt
    10321 ctggcgatcc agaagggcgt gccgagcgcg tgcatcgtgc agccttcgga actgccggcc
    10381 aagacggcgg agatggacgg catcccctgg ctgccgcgca tcatcgccaa ggcgcgcgcc
    10441 aagctgcgcg gcgagatgga ccctgacacc atgttcggct gcggaggcga cagggctttc
    10501 ctctcgaagt tcgacattca tcccgccgac tttctgcgcg tggtctgggc cgccggggat
    10561 gaagatgaaa aaatcctgcg ccatgtgagg gagcgccagg cggccatcgc ctgacccggc
    10621 tcaatcgtgc gtgccattcc gcggcgccgc cacagccacc acaccgccgc cgtgcggggc
    10681 gccgaagcgg cagccaacct ggacttcagg aaaacaagac tcgcgcgtgt ccatgcccgg
    10741 gccgcggcgc gcgatcatgc cggcggcgaa gaacagcagc gcgcccgccg ccagcaccgg
    10801 cgtgaaataa gccgccagtc tgcggtcggc gatggaaagc gggcgcgcgg acctgatgcg
    10861 cccctcctcc agccttctca tcccctccag cagcacgctg acggctcctg acaggcgctc
    10921 ctccaggctg gtgtccgtgc gcgacatgga ggcgcccgcc tcatgcacca gcaccgcgag
    10981 cgcggccggc gtgtgctttt cccacaggcc gggtgaaaag gagaagccaa gctggttgct
    11041 ggagcgctca taggccagga gcaccgcgtc ggcatcgccg ctccaggcgc ggcgcagatc
    11101 gcgggcatgg cggcggatgt tgcggcctga tccgttgtag gaatccaccg agacccagaa
    11161 ggcgcagccc gcgcgcgccg atgccccagc gatttcccat gccagcacct gccgggtctg
    11221 cgcgccgaag gcccgcgcgt catcgtggat gccatcccgc ggcggctcca cggaccgggc
    11281 cgcgccggag cagagaacgg cggcgaggat gacgtgcggg aggcgggact tcatggaggc
    11341 gcggttttca caccgtgacg ccggaatgat aatccggagg atcaaaaaga aaacccgacg
    11401 gtgacatgaa gcatgccgct tttttcgccg gagcggcggc tggtgttgtg gccgtaatcc
    11461 acgcgcacgg ggccgaaggg cagcttgtag cggagtcccg cgccggcggc ctggcggaag
    11521 tcggtggaga tgtcgagggc gctggtgttg ctgccgcggc cgaggctgcc gatgtcgcca
    11581 aaaacggcga attcgaggtt gggatagatt tcatggctga actccagcgt gcccagcagc
    11641 gcggaggtgc cgccaagcgg agtgccgccc gtggtcagcg ggccaagctc gcgctcggcg
    11701 aaggcgcgca cgctgttcgg gccgccgttg aagacacgcg cgtcaatggg cagctcctcc
    11761 gctgcggcgc cctggatgct catgagcgcg ccgccaaggg tcaggcggga ttttttcgtg
    11821 accggcagat gccatgcgcc gcgcaggtcg gtgcgcagaa agcccacgct cgatcccatc
    11881 acatccagcg tggtctccag ccgcgcgctg aggaaccagc ccttcgtcgg cagcaccggg
    11941 ctgtcgcgga agtcgagcat catgctgccg ccgagcgtgg ccagggtgta ggtgcccggt
    12001 ccggtctcct cggagctgag cgtgtccgtg gagatggagt tcaccgaggc tccaaagaaa
    12061 gcggtgtacg agaagaacgg cgtgagcctg cgcgccagct cggcatgaag gctcgatccg
    12121 tagcgggtgt attcgtagag gccgaaggtc tccacagcca ggcgcacgga gccggagtgg
    12181 gatgagttga agacggccgg atccgtgagg ctgacgaagc cgagcggacc cgccgcgctc
    12241 cagttcagct cggtggccag ggtgacgccg gtgtcgcgca ggttggtgtt gcggtagttc
    12301 acgccggcct gcgggccgag gaatgtgtcg taacccgcct cgaagccgag cgtgcgcggc
    12361 tttgtctcct cgccggagat gacgaggtcc gccagcgcgg gctgctccgg attcccgggc
    12421 tggatgaccg gctccacatc cagccgcgcg aacatgccgg tgtccagagc gcggcggaaa
    12481 aggaaatccg tgtccttgct ggaataccat tcgccctcgg cctcgcggaa ccccgcttcc
    12541 agcacacgcc tggctccccg gctgaagccc tcatgcggca gagcgcgcgc cacgcgggcg
    12601 cgcgcgcccg gcgccatgcg cagggtcaca tccaccgttc cgccttggga accgagatgg
    12661 tattccgagc ttgtcaccga actcagccag ccgcggctga cggcgatgtg gttgagcctg
    12721 cgctccagcg cctggacggc ggcctcgttg aatggctggc caggagtttg cgcgatctcc
    12781 cggctcattt ccccgctcaa ttcatcaggg gcgccatcga gagccactcc gccaaagaca
    12841 aactgcggcc cgggtttgac ctcgatggcg agcgcgcgca agccgtccgc gccaggttcg
    12901 gaggccggcg tgagcgcggc ctccgccagc aggtagccct ccgagcgcag gcgccgggtc
    12961 agcaaggccg cgccgctgtt cagatcggaa tccacccaga gcgggtgccg cttgtccgcg
    13021 ccctcctttt ccagggcggg ccgcagcagg aagcgccgca tttcatcctc cggcagcacc
    13081 ctgccgcctg tccaggtcag cgcgccgaca cgcgccggcc ggccggtctc cgcgcgcaga
    13141 tggatgcggt cgttctcaat gttccagcgc acttcagcct ccggccatcc tgcgcgcaaa
    13201 agatgctggc gggtgaaaaa ggccaggtcg tcggccaggg gggcggtgat gccgccgcca
    13261 tgcagggtga gctggtcctt gagcatttgc tccagccctt cccaggcagt ccctggaatg
    13321 ccgtgaaccc tcagcgagac actcccctcc cgcagccgga aaggctctcc cgccggttca
    13381 ggttcggcac cccgcatggc tggggcgatc cccagccata gcaaaaggca gccgtgcagc
    13441 gcggcgatga gttttcctga ctggctgcgg gtgaccatgc gctggcgttt cgcccgcgcg
    13501 cgccgccttg tcaaatgtca cgcgccgcgc aaatctggcg ggcgtgtttg ccgctccggg
    13561 aatcgccgcg aacgagaggg ggatcaatcc cgccgcacct gccggatgag cgtctgcatg
    13621 acgccctgca cctcaagatt ccgcaccggg atgagatccg gaaaggcggg gttctcggct
    13681 ttgaggtaag gttttccatc tgagacgagg tagcgtttca gggtcgtttc gccatcaatg
    13741 agcgcggcca cgatgtcgcg cggacgaggc tcgcgagctt cgaggatgac cacatcgcca
    13801 tcgcagatgc tggcgccgat catggactcg ccgcgcactt tgagggcgaa gacgcggctg
    13861 ccgcgcgcca gaccgagcgt gttcacatcc acggagatgg agccgtccgc ctgctgctcc
    13921 tggttttcgg cataaccggc ggcgat
//
LOCUS       DAOMYJ010000133        13871 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Prosthecobacter sp. isolate AMR_MDS_5629
            NODE_1472_length_13871_cov_20.717646, whole genome shotgun
            sequence.
ACCESSION   DAOMYJ010000133 DAOMYJ010000000
VERSION     DAOMYJ010000133.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867850
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prosthecobacter sp. (wastewater metagenome)
  ORGANISM  Prosthecobacter sp.
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Verrucomicrobiaceae; Prosthecobacter.
REFERENCE   1  (bases 1 to 13871)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13871)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.30
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:34:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,742
            CDSs (total)                      :: 4,684
            Genes (coding)                    :: 4,676
            CDSs (with protein)               :: 4,676
            Genes (RNA)                       :: 58
            tRNAs                             :: 55
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13871
                     /organism="Prosthecobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1472_length_13871_cov_20.717646"
                     /isolate="AMR_MDS_5629"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1965333"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            236..1024
                     /locus_tag="PLP58_13845"
     CDS             236..1024
                     /locus_tag="PLP58_13845"
                     /inference="COORDINATES: protein motif:HMM:NF014691.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HRJ09656.1"
                     /translation="MKRKLTEKDIQTIHGLAFEGKKRPTPYRDGQNVIRNSASGAITY
                     LPPEAKEVAGLMGELVAWINDEIESRDLPVPVIAALAHYQFATIHPYYDGNGRTARLL
                     TTLILHRCGYGLKGIYSLEEYYARHLRGYYDALSVGPSHNDHLGRAEADVSGFVQYFC
                     EGMADAFAKVAARAEASSLSAPQDKAALLRELRPLQRQALGLFAKHRIVTSNELASYL
                     GLSPRQGRDQCAKWVSEGFVAVANASKKGRSYRLAERFEGAIVE"
     gene            1050..1565
                     /locus_tag="PLP58_13850"
     CDS             1050..1565
                     /locus_tag="PLP58_13850"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HRJ09657.1"
                     /translation="MSQTFIVEPLSPAKHCREEFDCGIEALNDFLRLRARKEMEAGTS
                     ACFVIVPEDKPTVIAGYYTLSAATVLRTALPESVTKKLPRYNELPATLLGRLARDLRF
                     KGQQIGERLMASMLHRSVRASEEVASWAIVTDPKDEKARAFYASFGFQPLTESRQFIT
                     MKSAAQWISAR"
     gene            complement(1552..1845)
                     /locus_tag="PLP58_13855"
     CDS             complement(1552..1845)
                     /locus_tag="PLP58_13855"
                     /inference="COORDINATES: protein motif:HMM:NF020265.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1778 domain-containing protein"
                     /protein_id="HRJ09658.1"
                     /translation="MPAVATARRSNRLVARVTSDDKALLERAAGLEGCSVAVFVISHV
                     RAAAEEIVRRHDTVRLSQTESRRFVEAILAPVKAPTKRMRTALDLHRKTVTER"
     gene            complement(1905..4103)
                     /locus_tag="PLP58_13860"
     CDS             complement(1905..4103)
                     /locus_tag="PLP58_13860"
                     /EC_number="1.1.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009966005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004450 - isocitrate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent isocitrate dehydrogenase"
                     /protein_id="HRJ09659.1"
                     /translation="MSAKIIYTLTDEAPLLATYSLLPIVKAFTKAAGIEVETRDISLA
                     GRILAQFGQQDDDLSYLGKLCLEPTTNIIKLPNISASVPQLKAAIAELQSKGYKLPDL
                     PENPQTDEEKEIKAKYAKVMGSAVNPVLREGNSDRRAPKAVKDYAKKHPHKMGTWSAS
                     SKTTVGTMGKDDFFSNEKSVTVNDVTEVSITFTGKDGTQKVLLPKLALKAGEIMDATK
                     MSKKALVAFFEQQIAKAKADGVLFSLHMKATMMKVSDPIIFGHAVSVFFKDLVAKHAA
                     TLAEIGVNFNNGFGDLIAKLDKLPADKKAEIEADIQAAYASGPALAMVNSDKGITNLH
                     VPSDVIVDASMPAMIRGGGKMWDAAGKEQDTLAVIPDSSYAGIYQATIDFCKKNGALD
                     PKTMGSVPNVGLMAQAAEEYGSHNKTFEAPADGTIKVTDTAGNVLFEHSVEQGDIWRA
                     CQTKDAPVQDWVKLAVNRARASQTPAVFWLDKNRAHDAQIIAKVEKYLKDHDLTGLDI
                     RILPPAEACTFSLERIAKGLDTISVTGNVLRDYNTDLFPILEVGTSAKMLSIVPLMNG
                     GGLFETGAGGSAPKHVQQFCEENYLRWDSLGEFFALAASFEHLADTFKNPKAKVLADT
                     LDAANGKFLENDRSPGRKLGTIDNRGSHFYLCLYWAQELAAQTADADLAKTFKPIAEA
                     LTTNETKIVAELLAVQGQKADVGGYYKPDNAKADAALRPSATLNGILVGG"
     gene            4244..4783
                     /locus_tag="PLP58_13865"
     CDS             4244..4783
                     /locus_tag="PLP58_13865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009965646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione peroxidase"
                     /protein_id="HRJ09660.1"
                     /translation="MKTLLTLSITAALAATACADGLYDIPLKDIDGKDTSLKAYEGKV
                     LLIVNVASRCGNTPQYNQLQALHQEFAKHGLSVLGFPCNDFGAQEPGSNDEIKEFCSS
                     KYKVTFPMFDKVVVKGADKHPLYEALSGKDSPFPGDVKWNFGKFLVGKDGKILARFEP
                     RTKPDAPEVIEAIKGALGK"
     gene            5009..6808
                     /locus_tag="PLP58_13870"
     CDS             5009..6808
                     /locus_tag="PLP58_13870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009964396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HRJ09661.1"
                     /translation="MSTVAVRSAALPPAIPGLVRRFRQPKKNIPQTKQDRDFILQAVR
                     AYAEETRLVPPVPIEELQEHTDKILAIHGIDPAYRDYTGVLLANETWRESLATVPFEK
                     RLLLMPKCLRVESKCPAPFDEFGLLCKQCGLCSIQDFQNEAEKLGYAVLVAEGSAIVM
                     SLIQTGKIEAIVGISCLPVLERTFPYVEAAAIPAVAVPLLQDDCIDTTVDVDWVWDYI
                     HLTSEDKTRRLNLNALHDEVKTWFTRDALDALLGKPTCQTEEIARDWLARAGKRWRPF
                     LTIAAYQALREDPEGPVSDAVKLAAVSVEAFHKASLIHDDIEDNDAERYGEPTLHSEH
                     GIPVALNVGDLLIGEGYRLLAMMEAPSHLRSACLKVAAEGQRELCRGQGAELLWNNHP
                     LALSNTQVLEIFRSKTAPAFEVALKIGAALASRIEDTEDALHAYSENLGIAYQIRDDL
                     DDLGDDSAAATNVSIRPSIILALLRERGKGQIKDTMEALWNGQSTELPEKAAIRQWAT
                     ETGAYDKATLMLETYKEAAIRSLQEVELPNLKGLLRRVIGKIFNELEIKGWCREFEQQ
                     QQNTAEREALRAEAAKAAEHLVPKVAVAAEAAV"
     gene            6916..7785
                     /locus_tag="PLP58_13875"
     CDS             6916..7785
                     /locus_tag="PLP58_13875"
                     /inference="COORDINATES: protein motif:HMM:NF019474.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HRJ09662.1"
                     /translation="MKMLPIFLLMLAAGTSAAETTTFKLWPSGAPDPAGYESKPEEEI
                     KREKDDGIRRVTNVSEPTLTFYPSSASDGTTVLVCPGGGYSILALDHEGTAVCEFLNL
                     QGYNAALLKYRVPRRDPANPSREPLQDAQRAMSLLRLRAGELGIKPDRIGILGFSAGG
                     HLAVMTALHANERTYKQDAALDAPDATPNFCIPVYPAYLVGKDDTFTLLPEVKVTPKS
                     PPMCLIHAHDDKGVTSASASALLYLEYKKLGLPCELHIYTQGGHGFGMKPKDMPVEDW
                     PFRVVEWMQAMGW"
     gene            7878..9287
                     /locus_tag="PLP58_13880"
     CDS             7878..9287
                     /locus_tag="PLP58_13880"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase"
                     /protein_id="HRJ09663.1"
                     /translation="MTPRLFRLLLPALLFAACSHAAPNIVYIISDDHAWTDYGFMGHK
                     QIETPNLDKLAARSAVFTRGYVPTALCRPALATFASGMYAHQHKVSGNDPAPLDTNTP
                     AKAKGKGKGAETAEYKALREKLIARMDSQPSLARLLGAQGYLSHQSGKWWEGGHQRGG
                     FTHGMTRGFPQPGGRHGDDGLKIGREGMEPVFEFIDHALAEKKPFFIWYAPFMPHTPH
                     TPPDRLFQKYKAKGIESDFVARYYAMVEWFDETCGQLMDRIEQKGLLENTLFVYVADN
                     GWIQSADNPGYAPRSKQSANEGGTRQPIMFSWPGVIQPGMRGEQLCSSIDMMPTALAA
                     AGAPIPEQLPGINLLPVLKSGAASPRTECFGETFAHDVADIDKPEATLLYRWVVEGKW
                     KLLLTYDGRLDRYAGSHPRTEKRPQLFDLLADPHEDKNLAKDNPEVVARLARRLQDWW
                     PVTGRQVLTQWTDAPGEWK"
     gene            9330..10100
                     /locus_tag="PLP58_13885"
     CDS             9330..10100
                     /locus_tag="PLP58_13885"
                     /inference="COORDINATES: protein motif:HMM:NF018178.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1080 domain-containing protein"
                     /protein_id="HRJ09664.1"
                     /translation="MKNLDILVTALCLLTAAHAQEEGFKSLFNGKDLAGWDGNPELWS
                     VQDGCITGKTNGPDHLSYNQFLIWRGGVLKNFELRAKIKVTGNNTGIQYRSKELPENG
                     KWSIGGYQCDVHPATPNNAMMYEERGRGIIAQNGQGVVVDPSGVKWLASEHDPVQADV
                     AEWHEYIVIAKGNHLVHKLDGKVTVDLLDFEEGRRALEGLLAFQIHRGPAMTVQIKDV
                     LLKELPEGGVIDFANHPVPSDAQIIEAKPPKAKGKKAK"
     gene            10194..11573
                     /gene="pgtP"
                     /locus_tag="PLP58_13890"
     CDS             10194..11573
                     /gene="pgtP"
                     /locus_tag="PLP58_13890"
                     /inference="COORDINATES: protein motif:HMM:TIGR00881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate transporter protein PgtP"
                     /protein_id="HRJ09665.1"
                     /translation="MTVLAGFIQHRRPMISILKPAPHVERLPAGRIDSEYSRLRRRVF
                     AGIFIGYAGYYLVRKNFSLAIPDILQHYPQYTKAELGTAMTGLSIAYGFSKFLMGSVS
                     DKSNPRFFMPLGLLLACAILAVFGLVREVYASLTLMITLQTLNGWVNGMGWAPCGKTM
                     VHWFSTRERGRAVSVWNVAHNVGGALVAVIALWGVMIFGDWGAKFYFNALVAAAIALL
                     ALVLMRDTPQSCGLPPIEEHKNDYPPDYSHEHERTFTFREIFFGHVFNNKLLWAIAVA
                     NAFVYFVRYGVVDWIPTYMQTAKNFSFKESTAAWVCFEWAAVPGTILCGWVSDKVFRS
                     RRAPATILFMIPTLLGLVVYGLNRQGPLWVDIAALITIGFFIYGPVMLIGLQALDMVP
                     KKAAGTAAGFTGFFGYVFGSAIAGTGVGWLADHWGWSGVIAAMCACCLLAIVFSALTL
                     HHRAESSRA"
     gene            11685..12119
                     /locus_tag="PLP58_13895"
     CDS             11685..12119
                     /locus_tag="PLP58_13895"
                     /inference="COORDINATES: protein motif:HMM:TIGR00738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HRJ09666.1"
                     /translation="MRLSLFSDYALRVMMFAALKDAPFNLSEVAEAYDISRNHLVKVV
                     NHLAKLGYLETRRGRGGGITLAMPAEQTRVGLLLRRTEGSPPIVECFDPARNTCRING
                     HCRLKGLLAGAMESFYASLDAHTLRDLVCGPPGARMISVLLS"
     gene            12116..13405
                     /locus_tag="PLP58_13900"
     CDS             12116..13405
                     /locus_tag="PLP58_13900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ09667.1"
                     /translation="MKLRALFLCLALPPLLCLRAEDPYEQAPILYSQTQPRDAAQRLE
                     KLMREGKADVDRTDAWTVMRGLMRHLQIPEESQVMVFSKTSKQNDRIHPQTPRVVYFG
                     DEAYIGYCLGGSIEVAAVDPRLGPVFYLLDPYVDEKQPLHFQRDASCLSCHGGVFSPG
                     VPGVLVRSVFPGPEGHPIMSQGSTVVDSTTPFESRWGGWYVTGRHGDSHHRGNVTATE
                     LPDNSCDMPVEKGANVTSLAAFFDTSAYPRATSDIVALMVLEHQTGVQNTLTRANHGA
                     LRAMHMQTSLQRELGEPVLEEPMGTALRIIEHCAEDVLDALLFKDEAPLPEGGIEGDG
                     AFQAAFLKKARRNADGRSLRDFQLHTRLFKYRCSYMIHSLTFEHLSPPLKQRVLARLA
                     AVLDGADTSGRHDHLGASERRHIRAILGDLLPPPVDG"
     gene            13591..>13871
                     /locus_tag="PLP58_13905"
     CDS             13591..>13871
                     /locus_tag="PLP58_13905"
                     /inference="COORDINATES: protein motif:HMM:NF014634.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008887 - glycerate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0031388 - organic acid phosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HRJ09668.1"
                     /translation="MRVLIACDKFKGTLSAGQVARIIREGLAASIPDAEFDLCPIADG
                     GEGTTEAVITATGGEWVEHPTEDARGRPRTARYGWLPAGGGAVMEMSMV"
BASE COUNT         2573 a         4548 c         4247 g         2503 t
ORIGIN      
        1 cgaacacccc cgccaaaaac ggccgcgcct tcaccgccgc ctcccgccat gccagtgtca
       61 ccccgcgctg atggccattg aggcttcgcg ggtggcggtg gaccggctgc cggtgacgca
      121 gcagagatcg cttcgctgcg ggagtcggca cggttgctga cgacgcacta ctcgacgcag
      181 atcgagggga accgactgac gctgccgcag gtggaggagg tgatcgcggg cggcgctgaa
      241 gcggaagctc accgagaagg acatccagac gattcatgga ctggccttcg agggcaaaaa
      301 gcggcccacg ccttatcgcg acggtcaaaa cgtgatccgc aacagcgcca gcggtgccat
      361 cacctacctg ccgccggagg caaaggaggt cgcggggctg atgggcgagc tggtggcgtg
      421 gatcaatgac gagatcgagt cccgcgacct gcccgtgccg gtcatcgccg cgctggcgca
      481 ctaccagttc gccacgattc acccttacta tgatgggaat gggcgcaccg cgcggctgct
      541 gacgacgctg atcctgcacc gctgcggcta cgggctgaag ggcatctact cgctggagga
      601 atattacgcc cggcatctgc gcggctacta cgacgcgctc tcggtgggtc cgagccacaa
      661 cgaccacctc ggtcgtgccg aggcggatgt gagcggcttc gtgcaatact tctgcgaagg
      721 catggccgat gcttttgcca aggtcgccgc ccgtgccgaa gcctccagcc tgtccgcgcc
      781 gcaagacaaa gccgcgctgc ttcgcgaact gcgaccgctg caacgccagg ccctcggcct
      841 cttcgcgaag catcgcatcg tcacctcgaa cgaactcgcc tcctacctcg gcctctcacc
      901 ccgtcagggc cgcgatcagt gcgccaaatg ggtgtcagaa ggttttgtgg ccgtcgctaa
      961 tgcttcaaag aagggacgaa gctaccggct ggcggagagg tttgagggtg cgattgttga
     1021 gtaatccagc cgaaaggcta aacagctaca tgagccaaac cttcatcgtc gagcctctca
     1081 gtccggcaaa acactgccgg gaggagtttg attgtggaat tgaggcattg aatgacttcc
     1141 tccggcttcg cgcccgcaag gagatggagg ccgggacttc cgcctgcttc gtgatagtgc
     1201 cggaggacaa gccaacagtg attgccggtt actacacgct gtcagcagcc acggtgctgc
     1261 gaactgccct gccagaatcg gtgaccaaaa agcttccgcg atataatgaa ctgccagcga
     1321 ccttgctggg ccgtttggct cgcgatttga ggttcaaagg acagcaaatc ggcgaaaggc
     1381 tcatggcaag catgctgcat cggtcagtga gagcttccga ggaagtcgcc tcgtgggcca
     1441 ttgtgaccga tcccaaggat gaaaaggcac gggcctttta tgccagcttt ggctttcaac
     1501 ccctgacgga gtcccgccaa ttcatcacaa tgaagtcagc ggcgcaatgg atcagcgctc
     1561 ggtgaccgtc ttgcgatgca ggtccaaagc cgtgcgcatg cgcttggtgg gtgctttgac
     1621 gggtgcgagg atggcttcca caaagcgacg cgactcggtt tgactcaatc tcaccgtgtc
     1681 atgtcgccgg acgatttctt ccgccgcagc acgaacatga gaaatcacga acacagcgac
     1741 ggaacaacct tccaatccag ccgcacgctc cagcaaagcc ttatcgtccg atgtaactcg
     1801 ggcaacgagg cgattcgagc ggcgggcagt ggcgacggca ggcatggcgt ttgtgtgcca
     1861 aagtgtgcca cacagtctgc tggccgcaag agtggatttt ggcctcaccc ccccaccaag
     1921 atcccattca gcgtcgcgct ggggcggagt gcggcatcgg ctttggcgtt gtcgggtttg
     1981 tagtagccgc cgacgtcggc tttttggcct tggacggcga ggagttcggc gacgatcttg
     2041 gtctcgttgg tggtgagggc ttcggcgatg ggtttgaagg tcttcgcgag gtcggcgtcg
     2101 gcggtttggg cggcgagttc ctgggcccag tagaggcaga ggtagaagtg gctgccgcgg
     2161 ttgtcgatgg tgccgagttt gcggccgggg ctgcggtcgt tttcgaggaa tttgccgttc
     2221 gcggcgtcga gggtgtcggc gaggactttg gccttcggat tcttgaaggt gtcggcaagg
     2281 tgctcgaagg aggcggcgag ggcgaagaac tcgccgaggc tgtcccagcg gaggtagttc
     2341 tcctcgcaga actgctgcac atgcttcggc gcggagccgc cggcgccggt ttcaaagagg
     2401 ccgccgccgt tcatgagcgg gacgatggag agcatcttgg cggacgtgcc gacttcgagg
     2461 atggggaaga ggtcggtgtt gtagtcgcgg aggacgttgc cggtgacgga gatggtgtcg
     2521 aggcctttgg cgatgcgttc caaagagaag gtgcaggcct cggcgggcgg gaggatgcgg
     2581 atgtcgagcc cggtgaggtc gtggtctttg aggtattttt cgaccttggc gatgatctgg
     2641 gcgtcgtggg cgcggttttt atcgagccag aagacggcgg gggtctgcga ggcgcgggcg
     2701 cggttgacgg cgagcttcac ccaatcctgc acgggcgcgt ctttcgtctg gcaggcacgc
     2761 cagatgtcgc cctgctcgac gctgtgctca aagaggacat tgcctgcggt gtcggtgact
     2821 ttgatggtgc cgtcggcggg ggcttcgaag gtcttgttgt ggctgccgta ttcttcggcg
     2881 gcctgggcca tgaggccgac attcggcacg ctgcccatgg tcttcggatc gagagcaccg
     2941 ttctttttgc agaagtcgat cgtggcctgg tagatgccgg cgtagctgct gtccgggatg
     3001 actgccaacg tgtcctgctc cttgccggcg gcgtcccaca tcttgccacc gccgcggatc
     3061 atggcgggca tggaggcatc gacgatgacg tcgctcggga cgtggagatt ggtgatgcct
     3121 ttgtcgctgt tcaccatggc caaggcgggg ccgctggcgt aggcggcctg gatgtcggcc
     3181 tcgatctcgg cttttttgtc ggcggggagc ttgtcgagct tcgcgatgag gtcgccgaag
     3241 ccgttgttaa agttcacgcc gatctcggcg agcgtggcgg cgtgtttggc gacgaggtct
     3301 ttgaagaaga cgctgacggc gtggccgaag atgatgggat cgctgacctt catcatggtg
     3361 gccttcatgt ggagcgagaa gagcacgcca tcggccttcg ccttggcgat ctgctgctcg
     3421 aagaaggcga cgagggcctt cttgctcatc ttggtggcgt ccatgatctc gccggctttg
     3481 agggcgagct tggggaggag gactttctgg gtgccgtctt tgccggtgaa ggtgatgctg
     3541 acctcagtga catcattgac ggtcactgac ttctcgttgg agaagaaatc gtctttgccc
     3601 atggtgccga cggtggtctt cgaactcgcg gaccaggtgc ccatcttgtg cgggtgcttc
     3661 ttggcgtagt ctttgacggc cttcggcgca cggcggtcgc tgtttccttc acgcagcacg
     3721 ggattcacgg cgctgcccat gaccttggcg tatttcgctt tgatctcctt ttcctcgtcg
     3781 gtctgcggat tttcgggcag atcgggcagt ttgtagccct tcgactgcaa ctcggcgatg
     3841 gcggccttga gctgcggcac ggacgcggag atgtttggca gcttgatgat gttcgtggtc
     3901 ggttcgaggc aaagtttgcc gaggtagctc aaatcatcgt cctgctggcc gaactgggcc
     3961 aaaatgcggc cggcgagcga gatgtcgcgc gtttcgacct cgatgcccgc cgccttggtg
     4021 aaagccttca caatgggcag cagcgaatac gtggcgagga gcggggcttc gtcggtgagg
     4081 gtgtagatga ttttggcgga catgtctatg tgcggggggg atgagcgcct gagtgctagc
     4141 gccagcgcgc gggaagcgca acgcggaagg cgtttccggt ccaaaatgca tcccgccact
     4201 ggagatgaaa cccttccgcc ccgccgctcg ttattcagtt cccatgaaga cacttctcac
     4261 cctctccatc accgccgccc tcgccgccac agcctgcgcg gacggactct acgacatccc
     4321 cctcaaggac attgacggca aggacaccag cctcaaagcc tacgagggca aagtcctgct
     4381 catcgtcaac gtggcttcgc gctgcggcaa cacgccgcag tacaaccagc tccaggccct
     4441 gcaccaggag ttcgccaagc acggcctgtc cgtccttggc ttcccctgca acgacttcgg
     4501 cgcgcaggag ccgggcagca acgatgagat caaggagttc tgctcctcga aatacaaggt
     4561 tacattcccc atgttcgaca aggtcgtggt caaaggcgcg gacaaacacc cgctctacga
     4621 ggcgctcagc ggcaaggact cgcccttccc cggtgatgtg aagtggaact tcggcaaatt
     4681 cctcgtcggc aaggacggca aaatcctggc ccgttttgag ccgcgcacca agcccgacgc
     4741 gccggaagtc atcgaggcca tcaaaggcgc gctcggcaaa taatgcggat gagccgcatt
     4801 gactccacgc gaaaaaaaag cgcggcctga caggccgcgc ttttttagtg gtaccaaggc
     4861 ccgcagcgca atttccgccc actgaggcgc aatccttgca agaccaccat cccgcgccac
     4921 ggttacagaa agacctccga agaccgattc tcaaaatgcc ccggcttgag aatccccccc
     4981 ttggagcttc gaatttcatt ttcactccat gtccaccgtc gccgtccgct ccgccgccct
     5041 gccgcccgcc attcccggac tggtacgccg tttccggcag cccaagaaga acatcccgca
     5101 gaccaagcag gaccgtgact ttatactcca agccgtgcgc gcctatgcgg aggagacccg
     5161 gctcgtgccg cccgtgccca ttgaggaact ccaggaacac acggacaaaa tcctcgccat
     5221 ccacggcata gaccccgcct accgggacta caccggtgtg ctgctcgcca acgaaacctg
     5281 gcgcgagtcc ctggccacag tgccatttga aaaacgcctc ctgctcatgc ccaagtgcct
     5341 gcgggtggag agcaaatgcc ccgcgccttt tgacgaattc ggcctgcttt gcaagcagtg
     5401 cggcctctgc tccatccagg acttccaaaa cgaggccgaa aagctgggct acgccgtcct
     5461 tgtcgccgag ggcagcgcca tcgtcatgtc tctcatccag acgggcaaga ttgaggccat
     5521 cgtcggcatc tcctgccttc ccgtgctcga acgcaccttc ccttatgtcg aggccgccgc
     5581 cattcctgcc gtggctgttc ccctgctcca ggatgactgc atagacacca cggtggatgt
     5641 ggattgggtc tgggactaca tccacctgac cagcgaggac aaaacccgcc gcctcaatct
     5701 caacgccctc cacgacgagg tcaaaacctg gttcacccgc gacgcgcttg atgcgcttct
     5761 tggcaagccc acctgccaga cggaggagat tgcccgcgac tggctggcgc gcgcgggcaa
     5821 acgctggcgc cccttcctca cgatcgccgc ctatcaagcc ctccgcgagg accccgaagg
     5881 ccctgtttct gacgctgtga aactcgcagc cgtctccgtc gaggcgtttc acaaagcctc
     5941 cctcattcac gacgacatcg aggacaacga tgcagagcgc tacggcgagc cgacactcca
     6001 ctccgagcac ggcatcccgg tcgcgctgaa tgtgggggat ctcctcatcg gcgaaggcta
     6061 ccgcctgctc gcgatgatgg aggccccctc gcacttgcgc tccgcctgcc tcaaagtcgc
     6121 cgcggaaggc cagcgcgagc tatgccgcgg tcaaggcgcc gaattgcttt ggaacaacca
     6181 cccgctggcc ctcagcaaca cccaggttct ggaaatcttc cgcagcaaga ccgcccccgc
     6241 ttttgaagtc gccctcaaga ttggcgccgc tctcgccagc cggatcgagg acaccgagga
     6301 cgcccttcat gcttacagcg agaatctcgg catcgcctac cagatccgcg acgacctgga
     6361 tgatcttgga gacgactccg ccgccgccac gaacgtcagc atccggccca gcatcatcct
     6421 cgccctcctg cgcgagcgcg gtaaaggcca gatcaaagac acgatggaag ccctttggaa
     6481 cggccagtcc accgagctgc cggagaaagc cgccatccgt cagtgggcca ccgagaccgg
     6541 cgcatacgac aaagccaccc tcatgctcga aacctacaag gaggccgcca ttcgcagcct
     6601 tcaagaggtg gaactgccaa atctgaaagg ccttctgcgc cgtgtcattg gcaagatttt
     6661 caacgagctg gagatcaaag gctggtgccg tgagttcgag cagcagcagc aaaacaccgc
     6721 cgaacgcgaa gccctgcgcg ctgaagccgc caaagccgcc gagcatctgg tgcccaaagt
     6781 ggccgtcgca gcagaagccg cagtgtgatc aggcggaatc gtgagccccc cgcggatctc
     6841 actcccggac gtaaagccga tgtgacggaa attcatcttg ccagaaggca tgaccgccgc
     6901 gcaggatgag tggccatgaa aatgcttccg atatttctcc tcatgctcgc cgccggcacg
     6961 tccgccgccg agaccacgac attcaaactc tggccctccg gcgcgcccga cccggcggga
     7021 tatgaatcca agccggagga ggagatcaag cgcgaaaagg acgacggcat ccgccgcgtg
     7081 accaacgtct cggagccgac cctgaccttc tacccctcgt ccgcttccga cggcaccacg
     7141 gtgctggtct gccccggcgg tggttacagc atcctggcac tcgatcatga gggcacggcg
     7201 gtgtgcgaat ttctgaacct gcaaggctac aacgcggcgc tgctcaaata ccgcgtgccc
     7261 cggcgcgatc cggccaaccc ctcacgcgag ccgctgcagg acgcgcagcg cgccatgagc
     7321 ctgctgcggc tgcgcgcagg tgagctgggc atcaagcccg accgcatcgg catcctcggc
     7381 ttctcggccg gcgggcacct cgccgtcatg accgcgctgc acgccaacga gcggacttac
     7441 aagcaggacg cggcgctcga cgcgccggac gccacgccaa acttctgcat cccggtctat
     7501 cccgcgtatc tcgtcggcaa ggacgacacc ttcaccctgc tgccggaagt caaagtcaca
     7561 cccaaatcgc cgcccatgtg cctcatccac gcgcatgacg acaagggcgt gaccagcgcc
     7621 agcgccagcg ccctgcttta cctggagtac aaaaagctgg gcctgccctg cgagctgcac
     7681 atctacaccc agggcgggca cggcttcggc atgaagccca aggacatgcc cgtggaggac
     7741 tggcctttcc gcgtcgtcga gtggatgcag gcgatgggtt ggtgagtccg cgcaggccgc
     7801 ttgccggatg cgacacattt tttgccggtg agacgggcag cggcgcgggc gttgtcttgc
     7861 gcgcaccgca tccagccatg acgccccgcc tttttcgtct cctcctgccc gccctcctct
     7921 ttgccgcgtg ctcccacgcc gcgccgaaca tcgtctatat catctccgac gaccacgcgt
     7981 ggaccgacta cggcttcatg gggcacaagc agatcgagac gccgaacctg gacaagctcg
     8041 ccgcgcgcag cgccgtcttc acccgcggat atgtgcccac cgcgctgtgc cgcccggcgc
     8101 tcgccacctt tgccagcggc atgtacgccc accagcacaa ggtcagcggc aatgatccgg
     8161 ccccccttga cacaaacacc ccggccaaag cgaagggcaa aggcaagggc gcggagacag
     8221 cggaatacaa ggctttgcgg gaaaagctga tcgcccgcat ggactcgcag ccctcgctgg
     8281 cgaggctgct cggcgcgcag ggatatctca gccaccagtc cgggaaatgg tgggagggcg
     8341 gccaccagcg cggcggcttc acccacggca tgacgcgcgg ctttccccag cccggcggca
     8401 ggcatggcga tgacggcctg aagatcgggc gcgaggggat ggagccggtg tttgagttca
     8461 ttgaccacgc gctcgcggag aaaaagccct tcttcatctg gtacgcgccc ttcatgccgc
     8521 acacgccgca cacgccgccg gaccggcttt tccagaaata caaggccaag ggcatcgagt
     8581 ctgactttgt ggcgcggtat tacgccatgg tggagtggtt tgacgagacg tgcggccagc
     8641 tcatggaccg catcgagcag aaggggctgc tggaaaacac cctcttcgtc tatgtggccg
     8701 acaacggctg gattcagagc gctgacaatc ccggctacgc gccgcgctcg aaacagtccg
     8761 ccaacgaggg cggcacgcgc cagcccatca tgttcagctg gccgggcgtc atccagccag
     8821 gcatgagggg ggagcagctc tgctccagca ttgacatgat gcccaccgcc ctcgccgccg
     8881 ccggagcacc catcccggag caactgcccg gcatcaacct gctgcccgtg ctcaaatccg
     8941 gggccgcaag cccgcgcacc gaatgtttcg gcgaaacctt cgcccatgac gtggcggaca
     9001 ttgacaagcc cgaggccacc ctgctctacc gctgggtggt ggaggggaaa tggaagctgc
     9061 tgctgaccta cgacggcagg ctcgaccgct acgccggcag ccatccgcgc acggaaaagc
     9121 gcccgcagct cttcgatctg ctggctgatc cgcatgagga taaaaacctc gccaaagaca
     9181 accctgaggt cgtcgcccgc ctcgcccggc gcctgcagga ctggtggccc gtcaccgggc
     9241 gccaggtgct cacccaatgg acagacgctc ccggcgagtg gaaataagcc ccgcttttga
     9301 tccgctcaaa caaaccccgt ttccacatca tgaaaaacct cgacatcctc gtcaccgccc
     9361 tctgccttct caccgccgcc catgcgcagg aggaaggctt taaatccctg tttaacggca
     9421 aagacctcgc cggctgggac ggcaatccgg agctgtggag cgtgcaggac ggctgcatca
     9481 ctggcaagac aaacggcccc gatcatctga gctacaacca gttcctcatc tggcgcggcg
     9541 gcgtgctgaa aaacttcgag ctgcgcgcga agatcaaggt caccggcaac aacaccggca
     9601 ttcaataccg cagcaaggag ctgcctgaga acggcaagtg gtccatcggc ggctaccagt
     9661 gcgatgtgca tcccgccact ccgaacaacg ccatgatgta cgaggagcgc gggcgcggca
     9721 tcatcgctca aaacggccag ggcgtcgtcg tggacccctc gggcgtcaaa tggctggcct
     9781 ccgagcacga tcccgtgcag gcagatgtgg ccgagtggca tgagtacatc gtcattgcga
     9841 aaggcaacca cctcgttcac aagctcgacg gcaaggtgac ggtggacctg ctcgatttcg
     9901 aggagggcag gcgcgcgctc gaaggtctgc tggcctttca aatccaccgt ggcccggcga
     9961 tgacggtcca gatcaaggac gtgctgctca aggaactgcc cgaaggcggt gtgattgact
    10021 tcgccaacca ccccgttccc agcgacgcgc agatcatcga ggcgaagccc cccaaagcga
    10081 agggcaaaaa ggcgaagtga tccggctggc gtcatgcgct gccggcgaca cacgcggata
    10141 aggcctcgat ccggcaaggc cgggacgcgc cccgcttccc tgaaggaaga gtcttgaccg
    10201 ttcttgccgg cttcatccag catcgccgcc cgatgatttc cattttaaaa cccgccccgc
    10261 atgtcgagcg cctgccggcc ggccgcattg actcggaata cagccggctg cggcgccggg
    10321 tgttcgccgg catcttcatc ggctacgccg gttactattt ggtgagaaaa aacttctccc
    10381 tggccatccc cgacatcctc cagcactacc cccagtacac caaggcggag ctgggcaccg
    10441 ccatgaccgg gctgagcatc gcctacggct tctcgaagtt cctcatgggc agcgtttccg
    10501 acaaaagcaa cccgcgcttc ttcatgcctc tcggcctgct gctggcctgc gccatcctcg
    10561 cggtcttcgg cctggtgcgc gaggtctatg catccttgac gctcatgatc acgctccaga
    10621 cgctcaacgg ctgggtgaac ggcatgggct gggcgccctg cggcaagacg atggtccact
    10681 ggttcagcac gcgcgagcgc gggcgcgccg tctccgtctg gaatgtggcg cacaacgtcg
    10741 gcggggcgct cgtggccgtc atcgcgctct ggggtgtcat gattttcggt gactggggcg
    10801 ccaaatttta cttcaacgcc ctcgtggccg ccgccatcgc cctgctcgcc ctcgtcctca
    10861 tgcgtgacac gccccagagc tgcggactgc cgcccatcga ggagcacaaa aacgactacc
    10921 cgcccgacta cagccacgag cacgagcgca ctttcacttt tcgcgaaatc ttcttcggcc
    10981 acgtcttcaa caacaagctg ctctgggcca tcgccgtggc gaacgcgttt gtctatttcg
    11041 tccgctacgg tgtcgtggac tggatcccca cctacatgca gacagcgaag aatttcagct
    11101 tcaaggagtc caccgccgca tgggtctgct ttgagtgggc cgccgttccc ggcaccattc
    11161 tttgcggctg ggtctcggac aaggtcttcc gctcccggcg cgctccggcc accatccttt
    11221 tcatgatccc caccctgctc ggccttgtcg tttacgggct gaaccgccag gggcctttgt
    11281 gggtggacat cgccgcgctc atcaccatcg gctttttcat ctacggcccg gtcatgctca
    11341 tcggattgca ggcccttgac atggtgccga aaaaggccgc cgggaccgcc gcgggcttca
    11401 ccggattttt cggttacgtc ttcgggagcg ccatcgccgg cacgggcgtc ggctggctcg
    11461 ccgaccactg gggctggagc ggtgtcatcg ccgccatgtg cgcatgctgc ctgctggcca
    11521 tcgtcttcag cgccctgacc ctccaccacc gcgccgaaag ctcccgtgca tgagctcggg
    11581 aacggcgcag cggcgccaag ggaggaaacg ggccgcgcaa aatacgcgca tggattgttg
    11641 catcgcgaat gcagatttgg aacccggccc gcgttgatct ccccatgcgc ctgagtctgt
    11701 tcagtgatta tgccctgcgg gtgatgatgt tcgccgcctt gaaggacgcc ccgttcaatc
    11761 tgagcgaggt ggctgaggcc tatgacatct cgcgcaatca ccttgtcaaa gtcgtcaacc
    11821 acctggccaa gctcggctat ctggagacgc ggcgcggacg cggcggcggc atcactctgg
    11881 cgatgccggc agagcaaacc cgcgtgggcc tgctgctgcg ccgcaccgag ggcagcccgc
    11941 ccatcgtcga gtgcttcgac cccgcccgca acacctgccg catcaacggc cactgccgac
    12001 tgaagggcct gctggccggc gccatggaga gcttttacgc ctcgctcgac gcccatacgc
    12061 tgcgcgacct ggtctgcggc ccgcctggcg cgcggatgat ctccgtcctg ctgtcatgaa
    12121 actccgcgcc cttttcctct gcctcgccct gccgccgctg ctgtgcctgc gcgcggagga
    12181 tccgtatgag caggctccca ttctttactc gcagacccag ccgcgcgacg ccgcccagag
    12241 gctggaaaag ctcatgcgcg aaggcaaggc cgatgtggac cgcaccgacg cgtggacggt
    12301 gatgcgcggc ctcatgcgcc atctgcaaat cccggaggag tcccaggtca tggtcttctc
    12361 caagaccagc aagcaaaacg accgcatcca tccgcagact ccgcgcgtcg tctatttcgg
    12421 cgacgaggcc tacatcggct actgcctggg cggctccatc gaggtggccg ccgtggatcc
    12481 gcggctcggc cccgtctttt acctgctcga cccttatgtg gatgaaaagc agccgctcca
    12541 ctttcagcgc gacgcgagct gcctgagctg ccacggcggc gtcttctcac ccggtgtgcc
    12601 cggcgtgctg gtgcgctccg tcttccccgg ccccgaaggc caccccatca tgagccaggg
    12661 cagcaccgtg gtggacagca ccacgccctt tgagagccgc tggggcggct ggtatgtcac
    12721 cggacgccat ggcgactccc accaccgagg caatgtcacc gccaccgagc tgcccgataa
    12781 ttcctgcgac atgccggtgg aaaaaggcgc gaatgtcacc agcctcgccg cattcttcga
    12841 cacctccgcc tatccccgcg cgacgagcga catcgtcgcc ctcatggtcc tggagcacca
    12901 gaccggtgtg caaaacaccc tgaccagggc caatcacggc gccctgcgcg ccatgcacat
    12961 gcagaccagc ctccagcgcg agctagggga gcccgtgctc gaagaaccca tgggcaccgc
    13021 cctgcgcatc atcgagcact gcgccgagga tgtgctcgac gcgctgctgt tcaaagacga
    13081 ggccccgctg cccgagggcg gcatcgaggg cgacggcgcc ttccaggccg ccttcctgaa
    13141 aaaagcgcgc cgcaacgccg acggccgctc cctgcgcgac ttccagctcc acacccgcct
    13201 cttcaaatac cgctgcagct acatgatcca ttccctcacc ttcgagcacc tcagccctcc
    13261 tttgaagcag cgcgtgctgg cccggctcgc cgccgtgctt gacggcgccg acaccagcgg
    13321 gcgccacgat catctcggcg ccagcgagcg ccgccacatc cgcgccatcc tcggagacct
    13381 gctgccgcct ccggtggatg gctgagaggg atgttggatt gcggatttca gaatgtggaa
    13441 tgggcggttg gcgggtgaat gagcgggcca tcggccattg acgctggcat ggatggggaa
    13501 cgggacttca aatccacatt ccgcaatccg cattccgcgc cccccaactt tcccgttgtc
    13561 cggccgggtg gctgctgata tgcggcgttc atgcgtgtcc tcatcgcctg cgacaaattc
    13621 aagggaaccc tctccgccgg ccaggtggcg cggatcattc gcgagggcct cgccgcgtcc
    13681 atcccggatg cggagtttga cttgtgcccc atcgcggacg gcggagaggg cacgaccgag
    13741 gcggtgatca ctgccaccgg cggtgaatgg gtggagcacc ccaccgagga cgcgcgcggg
    13801 cgtccgcgga ccgcgcgcta cggctggctg cccgcaggcg gcggcgcggt gatggagatg
    13861 agcatggtca g
//
LOCUS       DAOMYJ010000134        13867 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Prosthecobacter sp. isolate AMR_MDS_5629
            NODE_1473_length_13867_cov_19.808862, whole genome shotgun
            sequence.
ACCESSION   DAOMYJ010000134 DAOMYJ010000000
VERSION     DAOMYJ010000134.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867850
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prosthecobacter sp. (wastewater metagenome)
  ORGANISM  Prosthecobacter sp.
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Verrucomicrobiaceae; Prosthecobacter.
REFERENCE   1  (bases 1 to 13867)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13867)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.30
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:34:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,742
            CDSs (total)                      :: 4,684
            Genes (coding)                    :: 4,676
            CDSs (with protein)               :: 4,676
            Genes (RNA)                       :: 58
            tRNAs                             :: 55
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13867
                     /organism="Prosthecobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1473_length_13867_cov_19.808862"
                     /isolate="AMR_MDS_5629"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1965333"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..196)
                     /locus_tag="PLP58_13910"
     CDS             complement(<1..196)
                     /locus_tag="PLP58_13910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ09669.1"
                     /translation="MDDAELQLRLCACRPHGQDADDPLMRAALDSLPRHPGLEAWHQQ
                     QTDFDRAMCRHVGQAPVPARL"
     gene            complement(189..725)
                     /locus_tag="PLP58_13915"
     CDS             complement(189..725)
                     /locus_tag="PLP58_13915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007413405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HRJ09670.1"
                     /translation="MDFEQLVGEHYQPLYRFAHSLAQNEADAADLTQQTFLRWATRGF
                     QLRERSKAKTWLFTTLYREFLSSRRRGRRFPHLELDDAESELPSVAPRVVEEMDSGAA
                     LDALSRLDDTFRAPLTLFYLQQHSYQEIADILGVPIGTVMSRLSRGKAQLKKLLSTLS
                     DAGAKVIPFEPEQRERHG"
     gene            complement(766..900)
                     /locus_tag="PLP58_13920"
     CDS             complement(766..900)
                     /locus_tag="PLP58_13920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ09671.1"
                     /translation="MKAILILALAATGLALSSCASCKECCAKKTDCATCKSGTCTHKH
                     "
     gene            complement(1025..3676)
                     /locus_tag="PLP58_13925"
     CDS             complement(1025..3676)
                     /locus_tag="PLP58_13925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009958427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1553 domain-containing protein"
                     /protein_id="HRJ09672.1"
                     /translation="MRRLSILLALSAPLSSGAEESLTFEKNVRPILKTHCFHCHGEDG
                     ETKGGLDVRLARFLLQGGKSGPAIQPGDAAGSHLVEMLQSGDMPKGKAKLRDAEIATI
                     EQWISQGAKTARAEPEKLGPEHAFTDEERAWWAFQPIRKPALPPFRAPQSTIRNPVDG
                     FIAAKLEENGLEFSPQADAATLLRRLSFAITGLPPAPEEVEAFVKASAVSPEGAFGAA
                     LARLLASPAYGERWGRHWLDIAGYADSDGYTDKDLERKWAWKYRDYVVAALNKDKPFD
                     EFIREQLAGDEMVPQPHKNLAPEAIEKITATGFLRMAADGTGAMNDNAARNSTIADTI
                     KIIGSAFYGMTLDCAQCHDHRYDPITQADYYRLRAVFEPGFDTKNWRTPAGRLVSLLT
                     DSQRAEAAKIEEEAKKVDAERLKKQEEFISEVLEKELAKAEENLRDTLRAAYRTEAKK
                     RTAEQTALLKKHPRVNQLSAGSLYLYDVTFKTRHADTLKKMTEDANAIRATKPAEEFL
                     HAFTEAPKKADAIPATFIFHRGDIEQPKDKVRPGDLTVLAGRRQVEVPEKDPALPTSG
                     RRLAFAKSLTDGKHPLVARVIVNQVWKRHFGKGLVNTPHDFGQLGERPSHPELLDWLA
                     AEFMEKGWSLKHLHSLILTSRVWQQSSLRDEKRDLIDPDNRLLSRMNVQRLEAETLRD
                     SFLAVSGRLNANAGGQPVPVTYSEEGQTIIGVDTRDTAGRQTGKYVSLNGEEYRRSLY
                     IQARRSMPLEMFAAFDAPAMTEANCAARPVTTVSPQSLLLMNNTTMREHAQDFARRVL
                     AEAGDDVEKQAARAWSLAYGRSPSMADLHESSAFIQAQTAHYQEHPAKLERSIGPAEK
                     EDAPPALLGLAALCHALLSSNGFLYVD"
     gene            complement(3749..5017)
                     /locus_tag="PLP58_13930"
     CDS             complement(3749..5017)
                     /locus_tag="PLP58_13930"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018968880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004350 - glutamate-5-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HRJ09673.1"
                     /translation="MTDSEIQQAILDMGRRARAAAHELVKLTTAKKNAILLKMADEIE
                     AREAGILAANEKDLERACANGLGSAMVDRLTLNPKRLKAIADAVREVAALPDPVGELL
                     SEWTRPNGIRIRKVRVPIGVIGIIFESRPNVTSDAASLCFKTGNATLLRGGSEAIDSN
                     IALAAALQAGGERAGLPKDSIQLIPFTDRASVEHMARMDQHMDLIIPRGGKGLIEKVV
                     ATARMPVIKHYDGVCHIYVHADADLEMAASLVINSKCQKPGVCNALETLLVDAAVAAE
                     FYPIVGARLTEAQVEIRADETARALLNVPSVPATEEDWRAEYLDLILAVKTVSGLDEA
                     VTHINTHGSHHTDSIVTRDRAAAERFQHEVDSAVVLHNASTRFNDGGEFGFGAEIGIS
                     TDKLHARGPMGLAELCSYKYVVDGAGQVRG"
     gene            complement(5103..6320)
                     /locus_tag="PLP58_13935"
     CDS             complement(5103..6320)
                     /locus_tag="PLP58_13935"
                     /inference="COORDINATES: protein motif:HMM:NF024753.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HRJ09674.1"
                     /translation="MRRLLFLLALPALHAADWPQFLGPMRDARAAADEPAPPASFARE
                     PEVLWKKSAGSGFAGPVVSGGKLILFHREGGDMTTEALDARTGAVLWRSVYVTDYVDS
                     FGFDNGPRAVPAIADGRVLTHGPEGRVTALDLATGRELWAYDTAAKAGSEQGFFGRAP
                     SPLVIGEKVFIAAGGEIGGQPAGVIALDVKTGAPVWHSADDEAGYASPAHEGNHLLCW
                     MRNRLWLLDAPSGRVLHSLPLRSRMDASVNAATPLPCGGGRWFVSAGYGVGANLFEIT
                     SKQELKEVWSRQDALDCHYATPVFHDGHLYGFHGRQETGMKLRCIRLRDGATAWEAPE
                     NLPGGTLLLVKDRLLIVTEQGELWIAPATPERFEKSMSVQILRAGHRSHAAYADGVLH
                     ARDSEQIVAVPLR"
     gene            6430..6933
                     /locus_tag="PLP58_13940"
     CDS             6430..6933
                     /locus_tag="PLP58_13940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009964236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="8-oxo-dGTP diphosphatase"
                     /protein_id="HRJ09675.1"
                     /translation="MQQDRTPPDWNHWQPTMRATLLFIHDLDLGRVLLIRKKRGLGAG
                     KVNGPGGKLDPGETPAECAVRETREELGVTALDPAKRGELWFQFVDGLALYVDVFLAT
                     RWQGEPFETDEAAPLWTAVDAIPFDEMWADDRFWLGGMLTGEKNFTGKFLFDGDDMLT
                     NEMTWHA"
     gene            6988..8508
                     /locus_tag="PLP58_13945"
     CDS             6988..8508
                     /locus_tag="PLP58_13945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009964613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arylsulfatase"
                     /protein_id="HRJ09676.1"
                     /translation="MLRHLLPCLALALTALPAAEKPNIIVIIADDLGYGDLGCYGAKT
                     IPTPHLDRLAAGGLRFTSGYCSASTCTPTRFSLLTGTYAFRQERTGIAPPNAPAIIKP
                     GTETVASLLKKAGYQTAAIGKWHLGLGDPAPDWNGELKPGPLEIGFDHCFLLPTTNDR
                     VPQVFVHDHRVPDLDPADPLWVGDKAPAGEHPTGVTHRHTLKMDWSHGHNQTIHNGIS
                     RIGFYTGGHKARFRDEDLADEWVRRSSTWIESCKDRPFFLYLASHDIHVPRIPHERFQ
                     GTTPHGFRADSVVQLDWCVGEVMKTLERLHLTDKTLVIFCSDNGPVLDDGYKDDAVEK
                     LGAHQPAGPYSGGKYSVLEGGTRTPFIVHWGGRVKPGVSDEVVCTVDLPASLAALAGA
                     PLPESACLDSQNLLPALLGEPGAKGRDHLVQQDNGVSGNFGLRAGEWKLVRMKKPGRT
                     QAKVTLKGRAENPPMHALYHLPADPGEEKDVSAEHPEKTRELIEKLDTILAAGRSR"
     gene            8775..9362
                     /locus_tag="PLP58_13950"
     CDS             8775..9362
                     /locus_tag="PLP58_13950"
                     /inference="COORDINATES: protein motif:HMM:NF018257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfocyanin-like copper-binding protein"
                     /protein_id="HRJ09677.1"
                     /translation="MRQLLPILPFLAAAALHSQTPPPAGHEHGGVTIPGLKHELFEGV
                     REEDLLRPGNDPKSLRVTLVATFNAANYGMNFNGWSHGKAVLSVPLGWRVDVTFINPS
                     PIPHSAIVIEKADTKKLQVAEPWFEGGATPKHLTGQTVSKAEFSFTPDEEGEYALACG
                     FPAHAVAGHWVALNVIAADGQPALQLGDKEPVVVK"
     gene            complement(9369..10541)
                     /locus_tag="PLP58_13955"
     CDS             complement(9369..10541)
                     /locus_tag="PLP58_13955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009961666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terpene cyclase/mutase family protein"
                     /protein_id="HRJ09678.1"
                     /translation="MKSTPALAFALALPQLALSQAATQPARHESLKQEIRLAYDRGLA
                     FLRAQQDARTGQWGDAEPVAFTGLAAASFLLNPERGPDSPAPVEAEKGIAFLLKNVQP
                     DGGIYVKARANYNTSLALMALLLHPKLENEEVMLAARRFIIGQQNDFDEKGKTDNPFD
                     GGVGYGTPKPGVPAHADLSNTHFALEALHHAQALLADKGDAGKNEPQLNFSAAIKFIE
                     NCQNRPETNKAGWVSDDPKDRGGFIYSPGETRGGETKTADGRTALRSYGSISYAGLLS
                     FIYAGLDKNDPRVKAVLDWLSENYTLDENPGLGAEGLFYYYHTMAKGLAVAGVDFIKT
                     RDGRVIDWRADLAQKLLNLQKEDGSWANDAGRWMESDRVLTTSYILMALARVHASL"
     gene            complement(10538..11341)
                     /locus_tag="PLP58_13960"
     CDS             complement(10538..11341)
                     /locus_tag="PLP58_13960"
                     /inference="COORDINATES: protein motif:HMM:NF040466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdjY domain-containing protein"
                     /protein_id="HRJ09679.1"
                     /translation="MPRFAVIFLLLPLATLRAQESAPDGARQLAEAQARLKKISASEY
                     DLDGIRINAATREVRIPSKVELRQAPIEYLLVHETGKTHETVLTTAVSPTAVQVALLL
                     VNYQAATEGMLARVPPEERPKIWREEPPATPGANRVLISAEWEEKGKLKSLPLAQWMQ
                     NSDTREPPPDLDHWIFNGSYVDERGFIGEHEGSIIAVWLDRGALFNSTAEGAWDDQRW
                     ISLPKNIPEEGTPVAVVIKPVESPGPEAPKQGAKSPPSSSPLPSKQTPP"
     gene            complement(11491..12183)
                     /locus_tag="PLP58_13965"
     CDS             complement(11491..12183)
                     /locus_tag="PLP58_13965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009962067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metallopeptidase"
                     /protein_id="HRJ09680.1"
                     /translation="MTLMLILFFGTLALSGLASLRVRSAYNRYSQVRASSGLTGAEVA
                     ARILALNNIRDVTIHPTEGHLTDHYDPAHRRLVLSEENYHGTSVAALGVAAHEAGHAI
                     QHKQLYAPLKWRMAAVGITGIASQIVMWVPLIGMMGGLIPYHFAITIMTVAMGILMLF
                     QLVTLPVEFDATARAKSVLAGTGAVAPGTEFQAMSKVLDAAALTYVAAFVSSLAYFLY
                     YLLRMTAGSREE"
     gene            12353..13078
                     /locus_tag="PLP58_13970"
     CDS             12353..13078
                     /locus_tag="PLP58_13970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ09681.1"
                     /translation="MEAAALPTSEEVIVCRVTRWYFRRMGILAGMLFLMGMYFLYDGK
                     YGYPAANKVAEEKEWFEQVLLKSYDEAKAAGAEEAWAAGAAAKGWPTGKNGEPPRWIS
                     HAAAKGWPEKPHKYTDREIAEQFWWGGGTLCAALAVAVVLLLNRKKTLRAETEHWTTP
                     EGVTVRYADVFRVDKRPWDQKGLAYAWHKDAAGREKRAVIDDLKYQGAARVLDRILAG
                     FKGELIEKIPDPPDDAETTPPQA"
     gene            13174..13416
                     /locus_tag="PLP58_13975"
     CDS             13174..13416
                     /locus_tag="PLP58_13975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007418888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HRJ09682.1"
                     /translation="MADNIQDKVKDIIVEQLGVNPEQVTPEAKFIEDLGADSLDTVEL
                     VMAFEEEFGIDVPDEEAEKLQSVGDVIRYVEENSAE"
     gene            complement(13455..>13867)
                     /locus_tag="PLP58_13980"
     CDS             complement(13455..>13867)
                     /locus_tag="PLP58_13980"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: protein motif:HMM:NF014033.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008817 - corrinoid adenosyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_process: GO:0009235 - cobalamin metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ATP:cob(I)alamin adenosyltransferase"
                     /protein_id="HRJ09683.1"
                     /translation="ETREVVATAQPWLITLMGELATPPGLEGKYAATHPVFGADKTAW
                     LDDWVARLESGGALQFKGWALPGEAGVMSGALADVARTVCRRAERCVADLAGTDQQVP
                     NPEIARFLNRLADVLWLLARWEEKTVSEKQSGGL"
BASE COUNT         2368 a         4397 c         4665 g         2437 t
ORIGIN      
        1 gcagccgcgc cggcacgggg gcctgcccca catgccggca catggcgcgg tcaaagtccg
       61 tctgctgctg gtgccatgct tcaaggccgg ggtggcgcgg cagggaatcg agggccgcgc
      121 gcatcagcgg gtcatcggcg tcctggccgt gggggcggca ggcgcagagt cggagctgga
      181 gttcggcgtc atccatggcg ctcgcgttgt tcaggttcga aggggatcac tttggctccg
      241 gcatcggaga gggtggagag gagttttttg agctgggctt tgccgcgtga tagtcgtgac
      301 atgacggtgc cgatgggcac gccgaggatg tcggcgatct cctgatagct gtgctgctga
      361 aggtagaaaa gcgtgagcgg agcgcggaag gtgtcgtcaa gccgggaaag ggcgtcgagc
      421 gccgcgccgc tgtccatttc ctcgacgaca cgcggcgcga cggagggcag ctcggactcc
      481 gcatcgtcca gttcaagatg cggaaagcgc ctgccgcgcc tgcggctgga gaggaattcg
      541 cggtaaagcg tggtgaaaag ccaggtcttg gccttggagc gctcgcggag ctggaagccg
      601 cgcgtggccc agcgcagaaa cgtctgctgg gtgagatccg ccgcgtcagc ctcgttttgc
      661 gcgaggctgt gggcaaagcg gtagagcggc tggtagtgct cgccgaccag ttgctcaaaa
      721 tccatgacat ttcaggccgg gcgcaccgcg cggcgaccgg cgtgtttagt gtttgtgagt
      781 gcaggtgccg cttttgcagg tggcgcagtc ggtttttttc gcgcagcact ctttgcagga
      841 ggcgcaactg gagagagcaa gaccggtggc ggcaagggcg aggatgagga tggctttcat
      901 gatgtgtgtt ggcatgtgtt tggcgcggcc cggattgaaa ggacgcacga gggcatgagg
      961 acgcggagcg tgaattattc cgtgggcttg aaaaaaatca cagcccccgc ggcaaactcc
     1021 agactcaatc cacataaaga aacccgttcg agctgagcag ggcgtggcag agggcggcga
     1081 ggccgaggag ggcgggcgga gcgtcttcct tctccgccgg gccgatggag cgctcaagtt
     1141 tggcgggatg ctcctggtag tgcgcggtct gcgcttgaat gaaggcggag gactcgtgca
     1201 aatcggccat gctgggggag cgtccatagg cgaggctcca ggcgcgagcg gcctgctttt
     1261 ccacgtcatc accggcctcg gccagcacgc gccgggcgaa gtcctgcgcg tgctcgcgca
     1321 tggtggtgtt gttcatgagc aggaggctct gcgggctgac ggtggtgacg gggcgggcgg
     1381 cgcagttggc ctcggtcatg gcgggggcgt cgaaggcggc gaacatctcc agcggcatgg
     1441 agcggcgcgc ctggatgtag agactgcggc ggtattcctc gccgttgagg ctgacgtatt
     1501 tgccggtctg gcgtccggcg gtgtcgcggg tgtccacgcc gatgatggtc tggccctcct
     1561 cgctgtaggt gacgggcacg ggctggccgc cggcattggc gttgagcctg ccggaaacgg
     1621 cgaggaagga atcccgcagc gtctcggcct ccaggcgctg cacgttcatg cggctgagca
     1681 ggcggttgtc ggggtcaatg aggtcgcgct tttcatcgcg cagggagctt tgctgccaga
     1741 cgcggctggt gaggatgaga ctgtgcagat gtttgaggct ccagcctttt tccatgaact
     1801 cggcggcgag ccagtcgagc agttcggggt ggctggggcg ctcgccgagc tggccgaagt
     1861 cgtgcggtgt gttgacgagt cctttgccga agtggcgttt ccagacctgg tttacgatga
     1921 cgcgcgcgac gagcgggtgc ttaccgtcgg tgagggattt ggcaaaggcc agccggcgcc
     1981 cgctggtggg cagggcgggg tctttttcgg ggacctccac ctggcgccgg cccgccagca
     2041 cggtaaggtc gccaggccgc actttgtcct tcggctgctc aatgtctccc ctgtggaaga
     2101 tgaaggtggc agggatggca tcggcttttt ttggcgcctc ggtgaaggcg tggagaaatt
     2161 cctcggcggg tttggtggcg cggatggcgt tggcatcctc ggtcatcttt ttcagagtgt
     2221 cggcatgcct ggtcttgaaa gtcacgtcgt agagataaag cgagccggca ctgagctggt
     2281 tgacacgcgg atgttttttg agcagcgcgg tttgctcggc ggtgcgtttt ttggcctcgg
     2341 tgcggtaggc ggcgcgcagt gtgtcgcgca aattctcctc ggctttggcg agctcttttt
     2401 cgagcacttc ggagatgaac tcctcctgct tcttgaggcg ctcggcgtcc actttcttcg
     2461 cctcctcctc gatcttcgcg gcctcggcgc gctgcgagtc ggtgagcaat gacacgagcc
     2521 tgcccgcggg tgtgcgccag ttttttgtgt caaagccggg ctcaaagacg gcgcgcaggc
     2581 ggtagtagtc ggcctgggtg atggggtcgt agcggtggtc gtggcactgc gcgcagtcga
     2641 gcgtcatgcc gtaaaaggcg ctgccgatga tcttgatggt gtcggcgatg gtggagttcc
     2701 gcgcggcgtt gtcgttcatg gcgccggtgc cgtcggcggc catgcgcagg aagccggtgg
     2761 cggtgatttt ttcgatggct tccggcgcga ggtttttgtg aggctggggc accatctcat
     2821 cgcccgcgag ctgctcgcgg atgaactcgt cgaagggctt gtccttgttc aacgcggcca
     2881 ccacatagtc gcggtatttc caggcccact tgcgctccag atccttgtcc gtgtagccgt
     2941 cgctgtcggc atagccggcg atgtccagcc agtgccggcc ccagcgctcc ccataggcgg
     3001 gcgaggccaa gagccgcgcc agcgccgcgc cgaacgcgcc ctccggcgac accgccgagg
     3061 ctttgacaaa ggcctccacc tcctccggcg cgggcggcag cccggtgatg gcaaaggaca
     3121 gccggcgcag cagcgtggcc gcgtccgcct gcggggaaaa ctccagccca ttctcctcca
     3181 gcttcgcggc gatgaagcca tccacgggat tgcggatggt ggattgcgga gcgcgaaaag
     3241 ggggcagcgc tggtttgcgg atgggttgga aggcccacca tgcgcgctcc tcatcggtga
     3301 aggcatgctc ggggcccagc ttctccggct ccgcgcgcgc ggtcttcgcg ccctgcgaga
     3361 tccattgctc gatggtggcg atctccgcgt ccctcagctt cgccttgccc ttcggcatgt
     3421 caccggattg caacatctcc acgagatggc tgccagcggc gtcacccggc tggatggcgg
     3481 gacctgactt gccgccttgc agaaggaagc gcgccagtcg cacatcaagt ccgcctttgg
     3541 tctcgccatc ctcgccatgg cagtgaaaac agtgcgtctt cagaatgggg cgtacatttt
     3601 tttcaaaggt caacgactcc tccgcgcccg acgagagcgg ggcggacaag gcgagaagaa
     3661 tggaaaggcg gcgcatgatg ggatgggcat acgggcagga gacgcgcgct cttgcgagcg
     3721 caggagcgcg gcagttttcg cagccttgtc atccccgcac ctgtcccgcg ccatccacca
     3781 catatttgta gctgcatagc tcggccagtc ccatggggcc gcgcgcatgc agcttgtccg
     3841 tgctgatgcc gatctccgcg ccgaagccga actcgccgcc gtcattgaag cgggtgctgg
     3901 cgttgtgcag caccacggca ctgtccacct catgctggaa gcgctccgcc gcagcgcggt
     3961 cgcgcgtgac gatgctgtcc gtgtggtggg agccgtgtgt gttgatatgc gtgacggcct
     4021 cgtccaggcc ggagacggtt ttcacggcca ggatgaggtc cagatactcc gcgcgccagt
     4081 cctcctccgt ggcgggcacg gagggcacgt tgagcagggc gcgcgcggtt tcgtcggcgc
     4141 ggatctccac ttgcgcctcc gtcagccgcg cgccgacgat tgggtaaaac tccgccgcca
     4201 ctgccgcgtc caccagcagc gtctccagcg cgttgcacac gccgggtttc tgacacttcg
     4261 agttgatcac aaggctcgcc gccatttcaa gatcggcatc ggcgtggaca tagatgtggc
     4321 agacgccgtc gtaatgcttg atgacgggca tgcgagcggt ggccacgact ttctcgatca
     4381 gccccttgcc tccgcgcgga atgatgaggt ccatgtgctg gtccattcgc gccatgtgtt
     4441 ccacgctggc gcggtcggtg aaggggatga gctggatgct gtccttcggc agcccggcgc
     4501 gctcgccccc ggcttggagg gccgcggcga gggcgatgtt cgagtcaatc gcctcgctgc
     4561 cgccgcgcag cagggtggcg ttgcccgtct tgaagcagag ggaggccgcg tcgctcgtca
     4621 cattggggcg ggactcgaaa atgatgccga tgacgccgat gggcacgcgc actttgcgta
     4681 tgcggattcc gttgggtcgt gtccactcgc tgagcagctc gcccacgggg tcgggcagcg
     4741 cggccacctc gcgcacggcg tcggcgatgg ctttgagccg ttttggattc aaagtcagcc
     4801 ggtccaccat ggcgctgccc aggccgttgg cgcaggcacg ctccaggtcc ttttcattcg
     4861 ccgccagtat gcccgcctcg cgcgcctcga tctcgtcggc catcttcagc aggatggcgt
     4921 tttttttcgc cgtggtcagc ttcacgagtt catgcgcggc ggcgcgggcg cggcggccca
     4981 tgtcgaggat ggcttgctgg atttcagagt cggtcatgag ggcgcgcagc atagtcagcc
     5041 gcgccggggc atccagtgga atgagctttt gcgaagcctc caggcacgcg ggcaaaggtc
     5101 gttcagcgca ggggcacggc cacgatctgc tcgctgtcgc gcgcatggag gacgccgtcg
     5161 gcatatgcgg catggctgcg gtggccggcg cggaggattt gcacggacat ggatttctca
     5221 aagcgctccg gcgtggcggg cgcgatccac aactcgccct gctcggtgac gatgagcagg
     5281 cggtctttga ccaggagcag cgtgccgccg ggcagattct cgggcgcttc ccaggcggtg
     5341 gctccgtccc taaggcggat gcagcgcagc ttcatgccgg tttcctggcg tccgtggaag
     5401 ccgtaaagat ggccatcgtg gaaaacgggc gtggcatagt ggcagtcgag cgcgtcctgg
     5461 cggctccaga cctccttcaa ctcctgcttg gaggtgatct cgaaaagatt cgcgccgaca
     5521 ccgtagcccg cggagacaaa ccagcgcccg cctccgcatg gcaggggagt ggcggcattg
     5581 accgaggcat ccatgcgcga gcgcaggggc agcgaatgca gcacgcggcc ggagggcgcg
     5641 tcgagcagcc agaggcggtt gcgcatccag cagaggagat ggtttccctc atgcgcgggg
     5701 gaggcgtagc cggcctcgtc gtccgcgctg tgccagaccg gcgcgccggt tttcacatca
     5761 agcgcgataa cgcccgccgg ctggccgccg atctcgccac ccgcggcgat gaacaccttt
     5821 tccccgatga ccagcggcga gggcgcgcga ccgaaaaaac cctgctccga acctgccttc
     5881 gccgccgtgt cataggccca caactcccgc cctgtggcca ggtccagcgc cgtcacccgg
     5941 ccctccggcc cgtgagtaag gacgcgcccg tcagcgatgg cgggcacggc gcgcgggccg
     6001 ttgtcaaagc cgaagctgtc cacataatcg gtaacataaa ccgatctcca gagcaccgcg
     6061 ccggtgcggg catcaagcgc ctcggtggtc atgtcgccgc cctcgcggtg aaaaaggatg
     6121 agcttccccc cggaaaccac aggccccgcg aaaccggacc cggcggattt tttccacagc
     6181 acctccggct cccgcgcgaa gctggcgggc ggagccggct cgtcagcggc ggcgcgcgcg
     6241 tcacgcatgg gtccgaggaa ctgcggccag tcggcggcat gaagggcggg cagggccagg
     6301 aggaaaagca ggcggcgcat gatgggatag gtgtcggatg aatgaacgga tgggcgcgcc
     6361 cgccgcgggg cacaagttcg gaataaggct ccttgcctcc cggcccggct cttgctaggt
     6421 ggcgggcaga tgcagcaaga ccgcacccct cccgattgga accactggca gccgaccatg
     6481 cgcgccacgc tgcttttcat ccatgacctg gatctcgggc gcgtgctgct catccgcaaa
     6541 aagcgcggac tcggcgccgg caaggtcaac ggtcccggcg gcaagctcga ccccggcgag
     6601 acacccgccg agtgcgccgt gcgcgagacc cgcgaggagc tcggcgtcac cgcgctggat
     6661 cccgccaaac ggggcgagct ttggttccaa ttcgtggacg gcctggccct ttatgtggat
     6721 gtcttccttg ccacgagatg gcagggcgaa ccgttcgaga ccgacgaggc cgcgccgctc
     6781 tggaccgccg tggacgccat cccgttcgac gaaatgtggg ccgacgaccg cttctggctc
     6841 ggcggcatgt tgacggggga gaaaaacttc accggcaaat tcctcttcga tggcgatgac
     6901 atgctgacga atgagatgac ctggcacgct tgagcacggc ggcttttttg atagccaaaa
     6961 gcgccccagt ctgttctcat cgccgccatg cttcgtcacc tcctgccctg cctcgccctc
     7021 gcgctcaccg cccttcccgc ggcggaaaag cccaacatca tcgtcatcat cgcggacgac
     7081 ctcggctacg gcgacctcgg ctgctacgga gccaaaacca tccccacacc gcacctcgac
     7141 cgtctcgccg ccggcggcct gcgcttcacc agcggctact gcagcgcctc cacctgcacg
     7201 cccacccgct tctccctgct caccggcacc tacgccttcc gccaggagcg caccggcatc
     7261 gccccgccaa acgcccccgc catcatcaag cccggcaccg agaccgtcgc ctccctgctg
     7321 aaaaaagccg gctaccagac cgccgccatc ggcaaatggc atctcggcct tggcgacccg
     7381 gcgcccgact ggaacggcga gttgaagccc ggcccgctgg agatcggctt cgaccactgc
     7441 ttcctcctgc ccaccaccaa cgaccgcgtg ccccaggtct tcgtccacga ccaccgcgtg
     7501 ccggacctcg accccgccga cccgctctgg gtcggcgaca aagcccccgc cggggagcac
     7561 cccaccggcg tcacgcaccg ccacacattg aaaatggact ggagccacgg ccacaaccag
     7621 accatccaca acggcatcag ccgcatcggc ttctacaccg gcggccacaa ggcgcgcttc
     7681 cgcgatgagg acctggccga cgaatgggtg cggcgctcca gcacctggat cgagtcctgc
     7741 aaagaccggc ctttcttcct ctacctcgcc agccacgaca tccatgtgcc gcgcatcccc
     7801 cacgagcgct tccagggcac cacgccgcac ggcttccgcg ccgactccgt cgtccagctc
     7861 gactggtgtg tcggcgaggt catgaaaacc ctcgaacgcc tccatctcac cgacaagact
     7921 ctcgtcatct tctgctccga caacggcccc gtgctggacg acggctacaa ggacgatgcc
     7981 gtggaaaagc tcggcgcgca ccagcctgcc gggccttaca gcggcggcaa atacagcgtg
     8041 ctggaaggcg gcacccgcac ccccttcatc gtgcattggg gcggacgcgt gaaacccggc
     8101 gtctccgacg aggtcgtctg caccgtggac ctgcccgcca gcctcgcagc gctcgccgga
     8161 gcgcccctgc cggagagcgc ctgcctcgac agtcaaaacc tgcttcccgc cctgctcggc
     8221 gagcccggcg ccaaaggacg cgaccacctc gtccagcagg acaacggcgt cagcggaaac
     8281 ttcggcctgc gcgccggcga gtggaagctc gtgcgcatga aaaagcccgg gcgcacccag
     8341 gcaaaagtca cgctcaaagg gcgcgcggaa aacccgccca tgcacgccct ctaccacctg
     8401 cccgccgatc caggcgagga aaaagatgtc agcgccgaac acccggagaa aacccgcgaa
     8461 ctcatcgaaa aactcgacac catcctcgca gccggccgaa gccgctgacg cctgcgcgcg
     8521 gcgagggtga tgaaggacat gcgtcaatgc ggcggaatat acaaagcgtc accacctttt
     8581 gtgaattcaa gccattggag ttatgaagtg ctggagtatt gcaaatccaa cactccaaga
     8641 ctccatcgct ccaaacaagg aatcacaaat catgatgacg cggggcatat cccgcgattt
     8701 ggcagggatg tccgcacgtc cgcggcgcga aacactcgcc tcgacagcgc gtaaactccc
     8761 ggttatcccc atccatgcgc caactccttc cgatccttcc ctttctcgcc gccgccgctt
     8821 tgcattccca gacacctcca cccgccgggc atgagcatgg cggcgtcacg attcccggac
     8881 tgaagcacga gctgtttgag ggcgtgaggg aggaggatct gctccggccc ggcaatgatc
     8941 cgaagagtct gcgcgtcacc ctcgtggcca ctttcaacgc ggccaactac ggcatgaact
     9001 tcaacggctg gtcccacggc aaggccgtgc tcagcgtgcc gctcggctgg cgggtcgatg
     9061 tgaccttcat caatcccagc cccattccgc acagcgccat cgtcattgaa aaggcggaca
     9121 cgaaaaaact ccaggtggcc gagccgtggt tcgagggcgg cgccactccg aagcatctca
     9181 ccggtcagac ggtgtcgaag gcggagttca gcttcacccc tgacgaggag ggcgagtatg
     9241 cgctggcctg cggcttcccc gcgcacgcgg tggcgggtca ctgggtggcg ctcaatgtca
     9301 tcgccgccga cggccagccc gccctgcagc tcggcgacaa ggagccggtg gtggtgaagt
     9361 gagccgcctc aaagactggc gtggacgcgc gccagcgcca tgaggatgta gctggtggtg
     9421 agcacgcggt cgctttccat ccagcgtccg gcgtcgttgg cccaggagcc gtcctctttt
     9481 tgcaggttga gcagtttttg cgcgaggtcg gcccgccagt ctatgacgcg gccgtccctg
     9541 gttttgatga aatccacccc ggcgacggcg agtcccttgg ccatggtgtg gtagtaataa
     9601 aagaggccct ccgcgccaag gccggggttt tcatcaaggg tgtagttttc gctgagccag
     9661 tcgagcaccg ctttcacgcg cggatcgttt ttgtcgagtc cggcgtagat gaagctgagc
     9721 aggccggcgt agctgatgct gccgtagctg cgcagggcgg tgcggccgtc ggcggttttg
     9781 gtctcgccgc cgcgtgtctc gccggggctg tagatgaagc cgccccggtc cttgggatca
     9841 tcgctgaccc agccggcctt gttggtctcg gggcggttct ggcagttttc gatgaacttg
     9901 atggcggcgg agaagttgag ctgcggctcg tttttcccgg cgtcgccttt gtcggccagg
     9961 agggcctggg cgtggtggag cgcctccagg gcgaagtggg tgttggaaag gtcggcgtgg
    10021 gcgggcactc cgggcttggg ggtgccgtag ccgacgccgc cgtcgaaggg gttgtcggtc
    10081 ttgcccttct cgtcgaagtc attctgctgg ccgatgatga agcggcgcgc ggcgagcatg
    10141 acctcctcgt tctccagctt cggatgcagg agcagggcca tgagcgcgag ggaggtgttg
    10201 tagttggcgc gggccttcac atagatgccg ccgtcgggct gcacgttctt cagcaggaag
    10261 gcgatgcctt tctccgcttc aaccggcgcg ggcgagtcgg gcccgcgctc cggattcagg
    10321 agaaaactgg ccgccgctag gccggtgaag gccaccggct cggcatcccc ccactgcccg
    10381 gtgcgcgcgt cctgctgcgc gcgcaggaag gcgagaccgc ggtcataagc gaggcggatt
    10441 tcctgtttca aggactcatg ccgcgcgggc tgggtggcgg cctgggagag ggctagctgc
    10501 ggcagagcca gcgcgaaggc gagagcggga gtggatttca tggcggtgtt tgtttggaag
    10561 ggagcggtga ggatgagggc ggggatttcg cgccttgttt cggagcttcg ggcccggggc
    10621 tttccaccgg cttgatgaca acggcgacgg gcgtgccctc ctccgggatg tttttcggca
    10681 gggagatcca gcgctggtcg tcccaggcgc cctcggcggt cgagttgaaa agggcgcccc
    10741 ggtccagcca gacggcgatg atgctgccct cgtgctcgcc gatgaagccg cgctcgtcca
    10801 cataggagcc gttgaagatc cagtgatcga ggtcgggagg cggctcgcgc gtgtcgctgt
    10861 tttgcatcca ttgagccagc ggcaggcttt tcagcttccc cttctcctcc cactcggcgg
    10921 agatcaggac cctgttggcg ccgggcgtgg cgggcggctc ctcgcgccag atcttcgggc
    10981 gctcctccgg cggcaccctg gccagcatgc cctcggtggc cgcctggtag ttcaccagca
    11041 gcagcgccac ctgcacggcg gtggggctga cggcggtggt gagcaccgtc tcgtgcgttt
    11101 tgccggtctc atgcacaagc aggtactcga tgggcgcctg gcgcagttcc accttcgagg
    11161 ggatgcgcac ctcgcgcgtg gctgcgttga tccggatgcc gtcgaggtca tactcgctgg
    11221 cggagatttt tttcagccgc gcctgcgcct cggcaagctg gcgcgcgcca tcgggcgcgg
    11281 actcctgggc gcgcagggtg gcgaggggga gcaaaagaaa gatgaccgcg aaacgtggca
    11341 tgggccggag catagacgca ttctctggag gaacaaacgc cgcctcgcat tcccggctct
    11401 ccagcgggcg gacggacaaa tcccgcccga aaacaaaacc gggcggacac aaagcgcccg
    11461 cccggtggaa acaaagccgc gccggtttcc tcattcctca cggctgccgg cggtcatgcg
    11521 cagcagatag tagaggaagt aggccaggct gctgacaaag gcggccacat aggtcagcgc
    11581 ggcggcgtcg agcaccttgc tcattgcctg gaactccgtg ccgggggcca cggcgccggt
    11641 gcctgccagc acgcttttgg cgcgggcggt ggcgtcgaac tcgaccggca gggtcaccag
    11701 ttggaagagc atgaggatgc ccatggccac ggtcatgatg gtgatggcga agtggtaggg
    11761 aatgagtccg cccatcatgc cgataagagg cacccacatg acgatctggc tggcgatgcc
    11821 ggtaatgccc accgccgcca tgcgccactt cagcggcgcg taaagctgct tgtgctggat
    11881 ggcatggccg gcttcatgag cggccacgcc gagcgccgcc acagaggtgc cgtggtagtt
    11941 ttcctcggag agcaccaggc ggcggtgcgc ggggtcatag tggtcagtta ggtggccctc
    12001 ggtcgggtga atcgtcacat cccggatgtt gttgagggcc aggatgcgcg cggctacctc
    12061 ggctccggtg aggccggagg aggcgcgcac ctgcgagtag cgattgtaag cgctgcgcac
    12121 acgcagggag gcaaggccgg agagggccag ggtgccaaag aagagaatga gcatgagtgt
    12181 catgggattt gagggggtga tttttcgggt gatacgactc tgcgctcata tcgagcgttg
    12241 aaaagcggtc atggggaaaa tcacacccgt tttggaacag agcgcggagc ggaccctgca
    12301 tggatgaaaa tcggaggttg cgcggcctgc ggcgcggcat tgactcgcgc gcatggaagc
    12361 cgccgccctg ccaacctccg aagaagtcat cgtctgccgc gtcacgcgct ggtatttccg
    12421 ccgcatgggc atcctggcag gcatgctttt tctgatgggc atgtacttcc tctacgacgg
    12481 caaatacggc taccccgcgg ccaacaaggt ggcggaggaa aaggagtggt tcgagcaggt
    12541 gctgctgaaa agctacgacg aggcgaaggc cgccggcgca gaggaggcat gggccgccgg
    12601 ggccgccgcc aaaggctggc caacgggaaa aaatggcgag cctccgcgct ggatctcgca
    12661 cgccgcggcg aagggctggc cggagaagcc gcacaagtac acggaccgcg agatcgcgga
    12721 gcagttctgg tggggcggcg gcacgctctg cgcggcgctg gcagtcgcgg tggtgctgct
    12781 gctcaaccgc aaaaaaacgc tgcgcgctga aacggagcac tggaccacgc cggagggtgt
    12841 gaccgtgcgc tacgcggatg tcttccgcgt ggacaagcgc ccctgggacc agaaaggcct
    12901 ggcctacgcc tggcacaagg acgccgccgg gcgtgagaaa cgcgccgtca ttgacgacct
    12961 caagtaccag ggcgccgcgc gcgtgctcga tcgcatcctg gcgggcttca aaggcgagtt
    13021 gatcgagaaa atcccggacc ctccggacga cgcggaaacc accccgccgc aggcctgaca
    13081 aagcctgttt gacaaccagg aaaaaagagc gaattcgccc ttgccaaaca aaatcaagcc
    13141 gctacctctc cgccgcacat ccccttcccc attatggcag acaacatcca agacaaagtc
    13201 aaagacatca tcgtcgaaca gctcggcgtg aaccctgagc aggtgacccc cgaagccaag
    13261 ttcatcgagg atctcggcgc tgactccctg gacaccgtgg aactcgtgat ggccttcgag
    13321 gaggagttcg gcattgatgt ccccgacgag gaggctgaaa agctccagtc cgtgggcgat
    13381 gtcattcgtt acgtcgagga aaactccgcc gagtaacctc ccactcgcat cctttcaatc
    13441 cggtcaggcg ggcttcacag cccgcctgat tgtttttcgg acactgtctt ttcctcccag
    13501 cgcgccagca gccagagcac atcagccagg cggttcaaaa aacgcgcgat ctccgggttc
    13561 ggcacctgct ggtccgttcc cgccaggtcg gccacgcaac gctccgcgcg ccggcacacc
    13621 gtgcgcgcca catccgccag cgcgccggac atcacacccg cctcgccggg cagcgcccag
    13681 cctttgaact gcaaggcgcc cccggactcc aggcgcgcca cccagtcatc cagccaggcc
    13741 gttttgtccg cgccgaagac cggatgcgtg gcggcgtatt ttccctcaag gccaggcggc
    13801 gtcgccagct cccccatcag tgtgatgagc cagggctgcg cggtcgcgac gacctcccgc
    13861 gtctccc
//
LOCUS       DAOMYJ010000135        13836 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Prosthecobacter sp. isolate AMR_MDS_5629
            NODE_1479_length_13836_cov_19.493288, whole genome shotgun
            sequence.
ACCESSION   DAOMYJ010000135 DAOMYJ010000000
VERSION     DAOMYJ010000135.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867850
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prosthecobacter sp. (wastewater metagenome)
  ORGANISM  Prosthecobacter sp.
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Verrucomicrobiaceae; Prosthecobacter.
REFERENCE   1  (bases 1 to 13836)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13836)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.30
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:34:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,742
            CDSs (total)                      :: 4,684
            Genes (coding)                    :: 4,676
            CDSs (with protein)               :: 4,676
            Genes (RNA)                       :: 58
            tRNAs                             :: 55
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13836
                     /organism="Prosthecobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1479_length_13836_cov_19.493288"
                     /isolate="AMR_MDS_5629"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1965333"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(264..806)
                     /locus_tag="PLP58_13985"
     CDS             complement(264..806)
                     /locus_tag="PLP58_13985"
                     /inference="COORDINATES: protein motif:HMM:TIGR01656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HRJ09684.1"
                     /translation="MKRPAVFFDRDGVVNVSPGAGYVLRWEDFHFAPGIIEALRLCRE
                     RGYALILATSQQGVGKGLMTLAGLEEIHARMQACLAREEAAFDGIHACTCLASDPACT
                     CRKPSPEMLLRAAAEHGLELSRSWLVGDADRDIQMALNAGVARTVRIGHEPPCAVRAE
                     HTLADTSGLPELLRAALPEV"
     gene            complement(803..1945)
                     /gene="ribD"
                     /locus_tag="PLP58_13990"
     CDS             complement(803..1945)
                     /gene="ribD"
                     /locus_tag="PLP58_13990"
                     /EC_number="1.1.1.193"
                     /EC_number="3.5.4.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009965968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA];
                     GO_function: GO:0008835 -
                     diaminohydroxyphosphoribosylaminopyrimidine deaminase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HRJ09685.1"
                     /translation="MSDDTDQIDRHWMRHALDEGRRGVGLTSPNPPVGAVIVIEKEGM
                     IRGGNRLTGEIIGRGWHRRAGGPHAEIEALRDAAENGRDVRGATIYITLEPCSTQGRT
                     GPCTEALAAAGLRRVVWGARDPNPQHAGRAEALLREAGLEVTTGVLELECSGLIRPFA
                     KWVTTGMPYVIAKAAQSLDGRLTRPPGESQWLTSEAARAHGRSLRREVDAVLVGAETV
                     RQDNPRLTFRPGDANHATGPQPPKDEEFHAAEAARPQPWRVVLSRSGRLPAGAHLLTD
                     EHKDRTLVLPGPDLPSALRELAGRGIVSVLIEGGGDVLNQAFQHRLVDEVQWYVAARL
                     CGAGVPVLGRTSEWTASVELKNPQYSFFADDFCVRATPLWPNSSPA"
     gene            complement(1980..2858)
                     /locus_tag="PLP58_13995"
     CDS             complement(1980..2858)
                     /locus_tag="PLP58_13995"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009959946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HRJ09686.1"
                     /translation="MAALQTTLGIIYDYDQTLSPTYMQDETLFPHFGINPAQFWKRSR
                     ELVDEQGYDGELAYLKCMLDYLEMDRPTNDELRALGAKLRYYKGVPDVFTELNNVLTS
                     QHRLLGIKIEHYIVSSGLKALLDGSSLAPMVKRIFGCEFGEDAEGRISFPKRVISHTT
                     KTQYLFRINKGMLDPHEDVNDHMDPELRPIPFQNMIYVGDGPTDVPCFTLMKRYGGHA
                     IAVYNPDETNRSSFRKCYQLSALADRVKHIAPADYRPGSHLRLLLEEMILDIADGIVR
                     RKKLEIEEGTVSAPHF"
     gene            complement(2963..4720)
                     /locus_tag="PLP58_14000"
     CDS             complement(2963..4720)
                     /locus_tag="PLP58_14000"
                     /inference="COORDINATES: protein motif:HMM:NF019436.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ09687.1"
                     /translation="MKTIIHHLRHSSARILCGSLLALSLTGIPAVAQIGGVKVSTTTL
                     ITQQQADSISSRFSTTPDFAPAERNSRPTLVLLIHGGTSAPNSPLPFVEPEDDEKRPG
                     TVGYSRFYFDFPFVSGILGSTRLYTLRGEELDSANWKSEFLSSERSDQFALANDPSNT
                     ASRATTNAVGLVRLDGSRGIGEMSRTALREIRALVDEFARHSNRRPFVVLVGHSKGGL
                     VTRYLMSEPQGNVAGINLTADERSAIRRLRNQVRYCVTISSPHTGSPLPDYAIEFRES
                     SAAVVQGMVQAAWNTVRAAAATRGIPLPAASPVNVTTDIIRLMGNPDDLGHLTTQFWN
                     TMNNGPLHPSRMVRDDGTRIPFFLYGGRTPGAEFFATANMLGLGGPALNTLLPSHPQS
                     QVNHMTQALIGLDYALHNVVGGDWGRIRLAGAASKNLDIVRRAWPVFGIPNRMSNPGE
                     RVFLLGVEGAPTYYLRNQGDNETDSDGMVSIDSALGIGLFTGPSTIENLQVLNVPVPA
                     TVMEPWERNVRSPAPGQAAANAPFGSWYRMYSGPWNMQNHTTIVKRRELGVELNRLLR
                     IAGPLASATSGTTSIWPAR"
     gene            4890..5495
                     /gene="msrB"
                     /locus_tag="PLP58_14005"
     CDS             4890..5495
                     /gene="msrB"
                     /locus_tag="PLP58_14005"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033743 - peptide-methionine (R)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HRJ09688.1"
                     /translation="MKNRLPVLITLALLLHAACAEEKNSTSEKPMPKPEVTLTEEEWK
                     KRLTDEQYRVTRKAGTERPFGAIYEEFEKQGEGTYYCVCCGVELFTSKEKFHSGCGWP
                     SFYDASTAKNVLERPDHSHGMMRVETLCKRCGAHLGHVFEGESLSAHTPTKRRFCING
                     VALHYVPKGGEAPKLLELGTAAVEKKKEEVAREAGVEVKKP"
     gene            5550..6179
                     /locus_tag="PLP58_14010"
     CDS             5550..6179
                     /locus_tag="PLP58_14010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ09689.1"
                     /translation="MQRTAIIRICVLAFLALLAWWGWLKVRGDDAMTFVYVIAVGAAA
                     GFLVVKYVVPWFGDAVGTALFSSGEMVGEDASMKAAAKLAQGDYEGAIAEHEKSLAEN
                     PAQVFPVGEIAKICADKLNDPARALRVLRERLAGQEWKEDDAAFLRFRMVDIQLEKLR
                     DYAAAKTLLEGIIQDYPDSRHRANAYHRVNEVEQMEMKEAMSRRGQSPA"
     gene            6205..8583
                     /locus_tag="PLP58_14015"
     CDS             6205..8583
                     /locus_tag="PLP58_14015"
                     /inference="COORDINATES: protein motif:HMM:NF015714.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComEC/Rec2 family competence protein"
                     /protein_id="HRJ09690.1"
                     /translation="MSRFWQNFRAGPMLMLAFAAAAGIAGRDFWGGWWSVALAVMAGL
                     IFVPDLRRGRFPLSAALVALLFSMLHGWRLSGSFEHPLLAELRSMPEGESRAVLRGSL
                     LPLHDGAEAGQGLAVCHFHSMKPAGDDWRKTRGWVRVQLPPKFDLAVAGEYEIEGVLQ
                     APAPPLNPGQFDFAAFCLRKGWVAQMRASRVVLVERRRFAPRFAFIKAAESCRAWIIA
                     RLSHGIPEGSPDAAVILAMTLGISDAAGEEVEDAFRGSGTLHVFAISGLHVMMLAGIF
                     LWAARALGYQRGVVLVIALLFGYAFITGWRPSAARASVMASMLLMAPLFNRRNRLGNT
                     LGASALLLLLADTQNLFMPGFQLTFGVLLGIALLAVPMMEKLKPWYEMDPFLPPQVAS
                     RWQRWSVKARTWLAGLLTVSLSAWTGSLPFMLGHFDTITPVGLLANLLLVPMAGMCLS
                     CACASLMLSLVQLSWLSALANQANALLASLMVWTAGLFAGLPGASFSVDLRWEKSAAP
                     VELRVFHLPNGGQAAHLRGGDRHWLLDTGNERAWRSVLQPWFRSQGINRLDGLFLSHA
                     DIAHVGAAPLALDSMRPPKFHTSLLEPWPFDPPESSLRRLERLTPPGGPRWHRLALEA
                     ALTLGADHPVHVLVLHPAHGDRHEKADDRGLILMLARDGVRVLWMNDAGFATARRLLQ
                     RGTDLRCDILIQSQHKTDKDGLADLLPAASPQVVILGSPEPITTPDARLHLKNLCMQS
                     GIQLLETGVAGSVGMEFEQGAVLLKCFHDGREIRLVPANRNTSASSGLPAED"
     gene            complement(8560..9102)
                     /locus_tag="PLP58_14020"
     CDS             complement(8560..9102)
                     /locus_tag="PLP58_14020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009961010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavoprotein"
                     /protein_id="HRJ09691.1"
                     /translation="MSRIVLGITGSIAAHKAGDLASQLVKAGHEVTCVPTRSALEFVT
                     PATLATLSRRPVVVDLFAEKEGWQPGHIQLADEADLLLVAPATANILAAMAHGFANDA
                     LTAIALATRAPVLLAPAMNGRMWEHPATMANVETLQKRGARFIGPAEGMLACGYEGTG
                     RMSPVEEIVAAACSILGGKA"
     gene            9189..9686
                     /locus_tag="PLP58_14025"
     CDS             9189..9686
                     /locus_tag="PLP58_14025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ09692.1"
                     /translation="MSFFKRAEVLTLLALGAGAAVWVLAPDGGDGGEDEGQSPVSEPV
                     SELQILRCTLERDFGNARLDVEVRHRNSSPRPLILAPPDVRLLTEDGVEIPPFILPAE
                     RPPQIAARSTQEARLRYWLEAGHLEGELILDIRGLKAWVKSPVPLDLRDLENRKPRTW
                     QGRIE"
     gene            9778..11424
                     /locus_tag="PLP58_14030"
     CDS             9778..11424
                     /locus_tag="PLP58_14030"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HRJ09693.1"
                     /translation="MQPRRTNLLLLLLVLTPLALLAWLGTYLIRDAARSTDSAMQAVL
                     AERLAAADHQLVDDLRHLAEQLGQLAASAAPDASAAALGTHPWVVEAWASHAGGETRV
                     ALDKGTHTPSPGDDAASRSISLRQALAGYGPVDAADENQPFLDIQSTEPDRSRQTVWM
                     TFVKNSGYDIKGTRKHAAAPPSGLSGWHVTDGDFIYWLLDADKSLVCARLDSRQMREA
                     LYQRLPPPGLPEYPGRMELRTITGIALHTSGRQLWGSGGAPAAERKCSPPLSQWVLAY
                     SPAEKEFPQPYLFPILLGVSSGCLLVMALAWTFSRENERELRLAQQRVSFVNQISHEL
                     KTPLTNIQLYTEMAANRVEEAGDAIARRQLRVVEAETSRLDRLIQNVMNYARQQRDRL
                     SVIPREIVLDEVVGRAVGNWRLLLENKGLKISAGFGGPASMSADPDAIEQILGNLLSN
                     VDKYASHGKWVSIRTETAEGCARVIVEDRGPGIPAGKRRQIFEPFERLRSDLNEGVSG
                     TGIGLTISRELADLHGGTLEVCPQYKEGARFILTLPIKKP"
     gene            11421..12104
                     /locus_tag="PLP58_14035"
     CDS             11421..12104
                     /locus_tag="PLP58_14035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009964246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HRJ09694.1"
                     /translation="MKILIAEDDTHTREALREVLGMEGYEVFTASDGLQAVDFFRAVR
                     PDFVCLDVMMPGQNGYEACKQIRRLDESVPILFLTAKSEEIDTVLGLELGADDYMTKP
                     FGVKEIIARIRAITRRAAARGGIKNSDGEFQMDDLRIVASELRAYRGSTEIQLSPRDL
                     KVLRLLHERRGKVVDRNTLADEVWGVDYFPESRALDQHISQLRKRIEADPASPRIIRT
                     VHGAGYRYE"
     gene            12228..12959
                     /locus_tag="PLP58_14040"
     CDS             12228..12959
                     /locus_tag="PLP58_14040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ09695.1"
                     /translation="MHRFLIVLILSTLAADVSAQTPPPKPSPPNDGPGGKDKPPFRGD
                     GPKDRGGDWRGGFPGFGRPPMRHDGFDQLPEDERRKVREALDKVWHLPEVISARDEAM
                     RANEKMRDTIRECLKKSDPEAAAIVERLEPREPFDFRKLPPLPPTDSEAFPLALVNRM
                     GSELEAFSRPDRREETRGLHLRIMDRPEIKAALDRLRQTRGEERLQAMHELRKAYREA
                     VGAEFQAARERRAAEGGGVPPKKPD"
     gene            complement(13105..>13836)
                     /locus_tag="PLP58_14045"
     CDS             complement(13105..>13836)
                     /locus_tag="PLP58_14045"
                     /inference="COORDINATES: protein motif:HMM:NF025720.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4407 domain-containing protein"
                     /protein_id="HRJ09696.1"
                     /translation="DRLDALKRQVQQEQADQFVGQQNAIRETLKSQIDALLMQMKGLQ
                     DDIARLSADEQERIAGIRAEPRRDILTQTLALHHLFENGTEGGRFALIAYLVLCALFM
                     LVDTIPLVVKFFSKPGPYDTLLDCDEAKYDRERLAFLRGFDRYMKQLVDSPFLHITQN
                     RPLERALIEGVDRSRAAKAFLEHLMELEQAFQEKMRVERERLEAGGITGKARMLEEMA
                     ETFYADLRQRMEAFFRDDRRTPVQA"
BASE COUNT         2326 a         4305 c         4686 g         2519 t
ORIGIN      
        1 cgatcgggcg aagtgcggcc tgcgcgcggc cagcgggtcc gccgccagcg cgccgtcctg
       61 catcagtagc gaggccagcg ccgcgccgcc cagcccgcat gagctggtgg ccaaaaaatc
      121 gcggcgagtc tgggtgaggt attcctgcgg ggacatgggc gggacattta cgcgccacgg
      181 cacgcgcttc gtgcgcggag tatgccttcg cgcattccgt ccggggcggg ccaaagaaat
      241 gtcgtccgcg tttttgtggc gtgtcagact tccggcagcg ccgcgcgcag cagctccggc
      301 agcccgctcg tgtctgccag tgtgtgctcc gcccgcaccg cgcagggcgg ctcatgcccg
      361 atgcgcacgg tgcgcgccac accggcgttg agcgccattt gaatgtcccg gtcagcatcc
      421 ccgacgagcc agctccggga aagctccagc ccgtgctcgg cggcggcgcg caggagcatc
      481 tcgggactcg gcttccggca ggtgcaggcg gggtcggagg ccaggcaggt gcaggcgtgg
      541 atgccgtcaa aggccgcctc ctcccgcgcc aggcaggcct gcatcctggc gtgaatttcc
      601 tccaggccgg ccagcgtcat cagccccttg cccacaccct gctggctggt ggccaggatc
      661 agcgcgtaac cccgctcccg gcacagccgg agcgcttcga tgatgcctgg agcaaagtga
      721 aaatcctccc agcgcagcac ataacccgcg ccgggcgaga cattcaccac gccgtcacgg
      781 tcaaaaaaca cagcgggtct tttcatgcgg gggaggaatt cggccacagt ggagtggcgc
      841 gcacgcaaaa atcatccgcg aagaatgaat attgcgggtt tttcagttcc accgaggccg
      901 tccattccga cgtccggccc agcaccggca cgcccgcgcc gcacaagcgc gccgccacat
      961 accactggac ctcatccacc aggcggtgct ggaaggcctg attgagaaca tccccgccgc
     1021 cctcgatgag cacgctcacg atgccccgcc cggccagctc cctgagcgcc gaaggcaggt
     1081 ccggccccgg cagcaccagc gtgcggtcct tgtgctcgtc ggtgagcaga tgcgccccgg
     1141 cgggcagcct gccgctgcgg ctgagcacca cgcgccaggg ttgcggccgc gcggcctcgg
     1201 cggcatggaa ttcctcatcc ttcggaggct gcgggccggt ggcatggttg gcgtcccccg
     1261 ggcggaaagt caggcgcgga ttgtcctgcc gcactgtttc cgcgccgacc agcacggcat
     1321 ccacctcccg gcgcagactg cgtccgtgcg cgcgcgccgc ctcgctcgtc agccactggc
     1381 tttcccctgg cgggcgcgtc aggcgcccgt ccaggctctg cgccgctttg gcgatcacat
     1441 aaggcatgcc tgtggtgacc catttggcaa agggccggat cagccccgag cactccaact
     1501 ccagcactcc cgtagtcacc tccaggcccg cctccctcag cagcgcctcg gcgcgccccg
     1561 cgtgctgcgg attcgggtcc cgcgcccccc acacaacgcg gcgcaggccg gcggccgcca
     1621 acgcctccgt gcagggcccc gtgcgcccct gcgtcgagca gggctccagg gtgatgtata
     1681 tcgtggcgcc gcgcacatcc cggccattct ccgccgcgtc ccgcagcgct tcgatctccg
     1741 catggggacc acccgcacgg cggtgccagc cccggccgat gatttcccct gtcaggcggt
     1801 tcccgccccg gatcatccct tccttctcga tgacaatcac ggcgcccacc ggcgggttcg
     1861 gacttgtcag ccccactccc cgccgcccct catccagcgc atggcgcatc cagtggcggt
     1921 caatttgatc tgtgtcatca ctcatggcgc gatcattagc ccctgttcgg aagtcgccgt
     1981 caaaaatgcg gggcggagac ggtgccttcc tcgatttcga gctttttgcg ccggacgatg
     2041 ccgtcggcga tgtcgaggat catttcctcc agcagcaggc gcaggtggct gccggggcgg
     2101 tagtcggcgg gggcgatgtg cttgacgcgg tccgcgaggg cggagagctg gtagcatttg
     2161 cggaagctgg agcggttggt ctcgtcggga ttatagacgg cgatggcgtg gccgccgtag
     2221 cgcttcatga gggtgaagca gggcacgtcg gtggggccgt cgcccacata gatcatgttt
     2281 tggaatggga tgggccgcag ctcggggtcc atgtggtcgt tcacgtcctc gtggggatcg
     2341 agcatgcctt tgttgatgcg gaagaggtac tgggtcttgg tggtgtggct gatgacgcgc
     2401 ttggggaagc tgatgcggcc ttcggcgtcc tcgccgaatt cgcagccgaa gatgcgcttg
     2461 accatggggg cgagggagct gccgtcgagc agggctttga ggccggagga gacgatgtag
     2521 tgctcgatct tgatgccgag gaggcggtgc tggctggtga ggacgttgtt gagctcggtg
     2581 aagacgtcgg gcacgccttt gtagtagcgg agcttggcgc cgagggcgcg cagctcgtcg
     2641 ttggtggggc ggtccatctc caggtagtcg agcatgcact ttaaataggc cagctcgccg
     2701 tcgtagccct gctcgtccac aagctcccgg ctgcgcttcc agaattgggc ggggttgatg
     2761 ccgaagtggg ggaagagcgt ctcatcctgc atgtaggtgg gactgagcgt ctggtcgtag
     2821 tcgtagatga tgccgagggt ggtctggagg gcggccatgg ggaaggctac gcgcaaaacg
     2881 cgctgacaac agcgggaata tggcgtgaaa acacaaaccc cgcgaccggg cggccgcggg
     2941 gtttggtttt gattagcggg actcaacgcg ccggccagat gctggtggtg ccgctggtgg
     3001 cgctggcgag cgggccggcg atgcgaagaa ggcggttcaa ttcgactcca agctcgcggc
     3061 gcttcacgat ggtggtgtgg ttttgcatgt tccaggggcc ggaatacatg cgataccagg
     3121 agccgaacgg ggcgttggcg gcggcttggc cgggggcggg cgaccttaca ttgcgctccc
     3181 agggttccat cacagtggcg ggcacaggga cattgagcac ttgcagattt tcgatggtgg
     3241 acggcccggt gaagagaccg atgccgaggg cggagtcaat gctgaccatg ccgtcgctgt
     3301 cggtttcatt gtcgccctgg ttgcgcaggt agtaggtggg agcgccctcg acgccaagga
     3361 ggaaaacacg ctcaccgggg ttggacatgc ggttcgggat gccgaagaca ggccaggcgc
     3421 ggcggacgat gtcgaggttt ttgctggcgg caccggcgag acggatgcgg ccccagtcac
     3481 caccgacgac gttgtgcagt gcgtagtcga gaccgatgag cgcctgggtc atgtggttga
     3541 cttgggattg agggtgactg ggcagcaggg tgttgagagc cgggccgccc aagccgagca
     3601 tgttggctgt ggcgaagaat tcagcgcctg gagtgcggcc gccgtagagg aagaagggga
     3661 tgcgggtgcc gtcatcgcga accatgcggg aggggtgcag ggggccgttg ttcatggtgt
     3721 tccaaaactg cgtggtgagg tggccaaggt cgtcagggtt gcccatgagg cggatgatgt
     3781 cggtggtgac attcacagga gacgcggcgg gcagtggaat gccccttgtg gcagcggcgg
     3841 cacgcacggt attccaggca gcctgcacca tgccctggac gacggcggcg ctgctttcgc
     3901 ggaattcgat agcgtaatca ggcagcggac tgccggtgtg cggagaagaa atggtgacac
     3961 aataacgcac ttggtttcgc aggcgacgga tggcggaacg ctcgtcggcg gtgaggttga
     4021 tgccggccac gttcccttgg ggctcgctca tcaggtagcg ggtgacgagg ccgcctttgc
     4081 tgtggccgac gaggacgaca aagggacggc ggttgctatg gcgggcgaac tcgtccacca
     4141 gtgctcggat ttcccgcagc gcggtccgtg acatctcgcc aatgccgcgc gagccgtcga
     4201 gacgaacgag gcccacggcg ttggtcgtgg cacgactggc ggtgttgctt gggtcgttgg
     4261 cgagagcgaa ctggtcggag cgctcgctgc tcagaaattc gcttttccag ttggcggagt
     4321 caagctcctc tcctctgagc gtgtagagcc gcgtgctgcc gagaatgccg gagacgaagg
     4381 gaaaatcgaa gtagaagcgg ctgtaaccga ccgtgccggg acgcttttcg tcatcttctg
     4441 gctccacgaa aggcagcggt gagtttggag cggaggtgcc gccgtggatg aggaggacga
     4501 gggtggggcg gctgttgcgc tcggcaggcg cgaagtccgg cgtggtgctg aagcgggagc
     4561 tgatactgtc cgcctgctgc tgggtgatga gcgtggtcgt ggagactttc acgccgccga
     4621 tctgcgccac ggctggaatg ccggtaaggg aaagcgccag caggctgccg cagaggatgc
     4681 gtgcggagga gtggcggagg tggtggatga tggttttcat gggtgtggag gagtggtgat
     4741 gtttgaattt ttcgggaggg cttttgcctc ccttcaccac gcagatgcgc ggcccgaggc
     4801 ggacttgcgc agaatccgaa aaaaaatccg agcggcattc ccatgtccgg ggcaaacatg
     4861 gtggaatctg gttcgttatg atgtttgcga tgaaaaaccg cctccccgtt cttatcactc
     4921 tcgccctcct cctgcatgca gcctgcgcgg aggaaaaaaa ctccacttca gaaaaaccca
     4981 tgcccaaacc cgaagtcacc ctcaccgaag aagaatggaa aaaacgcctc acagacgagc
     5041 aataccgcgt gacgcgcaaa gcaggcaccg agcggccctt cggcgccatt tatgaggagt
     5101 tcgaaaagca gggggagggc acctattact gcgtgtgctg cggggtggag cttttcactt
     5161 cgaaggagaa attccactca ggctgcggct ggccttcctt ctacgacgcg tccacggcga
     5221 agaacgtgct ggagcgtcct gaccactccc acggtatgat gcgcgtggag acgctgtgca
     5281 agcgctgcgg cgcgcacctg gggcatgtgt tcgagggcga gtccctctcc gcgcacacgc
     5341 ctacgaagcg gcgcttctgc atcaatggtg tggcgttgca ttatgtgccc aagggcgggg
     5401 aggcgccgaa gctgctggaa ctgggcaccg cagccgtgga gaaaaaaaag gaggaggttg
     5461 ccagggaggc gggcgtggaa gtgaaaaagc cctgatttgc gcgaaagaca ggcgtgtcat
     5521 tttcccaaag tgccgggtaa actgcgatca tgcagcgcac cgccatcatc cgtatctgcg
     5581 tcctcgcttt tctggccctg ctcgcctggt ggggctggct gaaggtgcgg ggtgatgacg
     5641 cgatgacttt tgtctatgtc atcgcggtcg gggccgcagc gggtttcctg gtggtgaagt
     5701 atgtggtgcc ctggttcggc gacgcggtgg gcacggcgct gttttcctcg ggcgagatgg
     5761 tcggggagga tgcctcgatg aaggctgcgg cgaaactggc tcagggcgac tatgaagggg
     5821 ccatcgccga gcatgagaaa tcgctggcgg aaaatccggc gcaggtgttt cccgtcggcg
     5881 agatcgccaa aatctgcgcc gacaagctaa atgaccccgc gcgtgccctg cgtgtgctgc
     5941 gggagcggct ggccgggcag gagtggaagg aggatgacgc ggcttttctg cgcttccgca
     6001 tggtggacat ccagctcgaa aagctgcgcg actacgccgc cgcgaagacg ctgctggagg
     6061 gcatcatcca ggattatccg gacagccgcc accgcgccaa cgcctaccac cgggtcaatg
     6121 aagtggagca gatggagatg aaggaggcca tgtcgaggcg cggccagtcg cccgcctgag
     6181 gcatgaaccg agcgcgcctg gatcatgagc cgcttctggc agaatttccg ggcggggccg
     6241 atgctgatgc tcgcgttcgc ggcggcggcg ggcatcgcgg gcagggactt ttggggcggg
     6301 tggtggagtg tggcgctggc tgttatggcc ggcttgattt ttgtgccgga cttgaggcgc
     6361 ggacgcttcc cgctctctgc cgcgctggtg gcgctgctgt tctcgatgct gcatgggtgg
     6421 cgcttgtccg gcagcttcga gcatcctctg ctggcggagc tgcgttcgat gccggagggc
     6481 gaaagcaggg cggtgctgcg cggaagcctg ctgccgctgc atgatggcgc tgaggcaggg
     6541 caggggctgg ccgtttgtca tttccactcg atgaagccgg cgggtgatga ttggcggaaa
     6601 acgcgaggct gggtgcgcgt gcagttgccg ccgaaatttg atctggccgt cgcaggggaa
     6661 tatgagatcg agggcgtcct gcaagcgcct gcgccgccgc tgaatccggg gcagttcgac
     6721 ttcgccgcat tctgcctgcg caaaggttgg gtggcgcaaa tgcgcgcgtc acgggtcgtg
     6781 ctggtggagc gccgccgttt tgcgccgcgc ttcgccttca tcaaagcggc ggaaagctgc
     6841 cgcgcctgga tcatcgcccg gctttcgcac ggcattccag aggggagtcc ggacgcggcg
     6901 gtgatcctgg cgatgaccct gggcatctcc gacgcggcag gcgaggaggt ggaggacgcc
     6961 tttcgcggca gcggcacgct gcacgtcttc gccatcagcg ggctgcatgt gatgatgctg
     7021 gcgggcattt ttttatgggc ggcgcgcgct ctggggtatc aaagaggtgt cgtgctcgtc
     7081 atcgcgctgc tttttggcta cgccttcatc accggctggc gcccctcggc ggcgcgcgcc
     7141 tccgtgatgg cctcgatgct gctcatggcg ccgcttttca accgccgcaa ccgcctggga
     7201 aacacactcg gagcctccgc cctgctgctc ctgctggcgg acacgcagaa tcttttcatg
     7261 ccgggatttc agctcacttt cggagtgctg ctggggatcg cgctgctggc cgttccgatg
     7321 atggaaaaac tcaaaccctg gtatgagatg gacccttttc tgccgccgca ggtggccagc
     7381 cggtggcaga ggtggagtgt caaggcgcgc acctggctcg ccggtctgct gaccgtctcc
     7441 ctctccgctt ggacgggcag cctgcctttc atgctggggc actttgacac catcacgccc
     7501 gttgggttgc tggccaatct gctgctggtg ccgatggcgg gcatgtgcct ttcctgcgct
     7561 tgcgccagcc tgatgctgag tcttgtccag ctttcatggc tttcggcgct ggccaaccag
     7621 gccaatgcgc tgctcgcctc gttgatggtg tggacggcgg ggctgttcgc cggtcttccg
     7681 ggggccagtt tcagcgtgga cctgcgctgg gagaaatccg ccgcgccggt ggagttgcgc
     7741 gtctttcatc tgccgaatgg cggccaggcc gcgcacctgc gcggcgggga ccgtcactgg
     7801 ctgctcgaca cgggcaatga acgggcatgg cgctcggtcc ttcagccgtg gtttcgcagc
     7861 cagggcatca accgccttga tggcttgttt ctgagccacg cggacatcgc ccatgtcggc
     7921 gcggcgcccc tggctctcga ttcgatgcgt ccgccgaaat tccacaccag cctgcttgaa
     7981 ccctggcctt tcgatccgcc ggagagcagt ttgcggcggc tggagcggct gactccacca
     8041 ggcgggccgc gctggcacag gctggcgctt gaggccgccc tcacactggg cgcggatcat
     8101 ccggttcatg tccttgtcct gcatccagcg cacggcgacc ggcatgagaa agcggatgac
     8161 cgcggcctga tactcatgct cgcccgcgac ggcgtgcggg tgctgtggat gaacgacgcg
     8221 ggtttcgcca ccgcccggcg gctgctgcag cgcggcacgg acctgcgctg cgacattctc
     8281 atccaaagcc agcacaaaac cgacaaggac ggtctcgccg acctgctgcc cgccgcatcc
     8341 ccgcaggtgg tcattcttgg cagtccggag ccaatcacga cacctgacgc gcgtttgcat
     8401 ttgaaaaatc tctgcatgca gagcggcatc caactgctgg agaccggggt tgcaggcagc
     8461 gtgggcatgg agttcgagca gggggccgtg cttttgaaat gcttccacga cggccgggaa
     8521 atccggctgg ttcccgcgaa tcggaacact tccgcgagtt caggccttcc cgccgaggat
     8581 tgagcacgcc gccgccacaa tctcctccac cggcgacatg cgtccagtgc cctcatagcc
     8641 gcaggccagc attccctccg ccggcccgat gaagcgcgca ccgcgttttt gcagcgtctc
     8701 cacattggcc atcgtggccg gatgctccca catgcggccg ttcatggccg gggcgaggag
     8761 caccggcgcg cgcgtggcga gcgcgatggc ggtgagcgcg tcattggcga agccgtgcgc
     8821 catggccgcg aggatgttcg ccgtggctgg cgcgacgagc aggaggtctg cctcgtcggc
     8881 gagctggatg tgccccggct gccagccctc cttttccgcg aacaaatcca ctaccaccgg
     8941 cctgcgcgag agcgtggcca gcgtcgccgg tgtgacgaac tccagcgccg agcgagtcgg
     9001 cacgcaagtc acctcgtgcc ccgccttcac gagctggctg gccagatccc ccgccttgtg
     9061 ggcggcgatg gagcctgtga tgccgaggac gatgcgtgac atggttgcgt gacggctaaa
     9121 aggcaccgca cgcgcctgaa tgcaattttt cagtttgcat tccgcgcgct ttcacagcac
     9181 gccagttcat gagctttttc aaacgcgccg aagtcctcac cctgctcgcc ctgggtgctg
     9241 gtgccgctgt ctgggtgctc gcgccggatg gcggggacgg cggcgaggat gaggggcagt
     9301 caccggtttc ggagccggtt tccgaactgc aaatcctccg ctgcaccctg gagagggact
     9361 ttggaaacgc gcgcctcgat gtggaggtgc gccaccgcaa cagcagcccc cggcccctga
     9421 ttttggcccc gccggacgtc cgtctgctca cggaagatgg agtggaaatc cctcccttca
     9481 tcctgcctgc tgaaaggcct ccgcaaatcg ccgcccgctc gacgcaggag gcgcggctgc
     9541 gctattggct ggaggccggc cacctcgaag gcgagctcat tctcgatata cgcggcctca
     9601 aagcctgggt caaaagcccc gtgcctctcg atttgaggga tttggagaat cgcaagccga
     9661 ggacatggca gggcaggatc gaataacagc gcggcccgcc gggggcaatg cggaggcatt
     9721 ctccgcagat tcgcgctttt gcagtcctgg catgggatta tggtacgggt cactgcaatg
     9781 cagccgcgcc gcaccaatct tctgctcctg ctcctcgtcc tgacgcctct ggccctgctg
     9841 gcatggctgg gcacctattt gatccgggac gcggcccgca gcacggactc cgccatgcag
     9901 gcggtgctgg cggagcggtt ggcggcggcg gaccaccaac tcgtggacga tctccgccac
     9961 cttgccgagc agctcggtca actcgccgca tcagccgcgc ctgatgcctc ggcggcggcc
    10021 cttggcacgc atccctgggt ggtcgaggca tgggcctcgc atgccggggg cgaaacgcgg
    10081 gtggctctcg acaaaggaac gcacactccc agccctggag atgatgccgc gagccggtca
    10141 atctcgttgc ggcaggccct tgccggttat ggaccggtgg atgccgcgga tgaaaaccag
    10201 ccgtttttgg acatccagtc cactgagcct gaccgcagcc ggcagacagt gtggatgacc
    10261 ttcgtgaaaa acagcggtta tgacatcaag ggcacccgca agcacgccgc cgctccgcct
    10321 tcaggcctct caggatggca tgtcaccgac ggggatttca tttactggct gctggatgct
    10381 gacaaatccc tggtctgcgc caggctggac agccgccaga tgcgcgaggc actctaccag
    10441 cggctgccac cacccggact gcccgagtat cccggccgca tggagttgcg cacgatcacc
    10501 ggcatcgccc tgcacacatc ggggaggcag ttgtgggggt cgggcggcgc gccggcggcc
    10561 gagcgcaaat gctcgccgcc gctcagccaa tgggttctgg cttactcgcc ggcggagaag
    10621 gagttcccgc agccctatct ttttcccatt ctgctgggag tcagttccgg ctgcctgctg
    10681 gtcatggcat tggcgtggac cttttcgcgc gagaacgagc gggagctgcg cctggcccag
    10741 cagcgcgtca gctttgtgaa ccagatctcc catgagctga agacaccgct gacaaacatc
    10801 cagctctata ccgagatggc cgccaatcgt gtcgaggagg cgggggatgc catcgcccgc
    10861 aggcagctcc gtgtcgtcga ggccgagacc tcccgcctgg accgtctcat ccagaacgtc
    10921 atgaactacg cccgccagca gcgcgaccgg ctctccgtga ttccgaggga gatcgttctc
    10981 gacgaggtgg tcgggcgcgc cgtcggaaac tggcggctgc tgctggagaa caagggtctc
    11041 aaaatcagcg ccggctttgg cggcccggca tccatgtccg ctgatcccga cgccatcgag
    11101 caaatcctcg gcaacctcct ctccaacgtg gacaaatatg cctcccacgg gaaatgggtc
    11161 tccatccgca ctgaaaccgc cgagggatgc gcgcgtgtca tcgtcgagga tcgcggcccc
    11221 ggcattcccg ccggcaagcg ccgccagatc ttcgaaccct tcgagcgtct gcgctccgac
    11281 ctcaatgagg gcgtcagcgg caccggcatc ggactcacca tctcgcgcga gcttgccgat
    11341 ctgcacggcg gcaccctgga agtctgcccg caatacaagg aaggcgcccg cttcatcctc
    11401 accctgccaa tcaaaaaacc atgaaaatcc tcatcgccga agatgacacc cacacccgcg
    11461 aagccctgcg tgaagtcctc ggcatggagg gctacgaggt cttcaccgcc agcgacggtc
    11521 tccaggctgt ggatttcttc cgcgccgtgc gcccggactt tgtctgtctg gatgtcatga
    11581 tgcccggcca gaacggctac gaggcctgca agcagatccg ccgccttgac gagtccgtcc
    11641 ccatcctctt cctcaccgcc aaatccgagg agatagacac cgtgctcgga ctcgaactag
    11701 gcgccgacga ctacatgacc aagcccttcg gtgtgaagga gatcatcgcc cgcatccgcg
    11761 ccatcacccg ccgcgccgcc gcgcgcggcg gcattaaaaa cagcgacggc gaattccaga
    11821 tggacgacct gcgcatcgtc gcctccgaac tccgcgccta ccggggctcc accgagattc
    11881 agctcagccc gcgcgatttg aaggtgctgc gcctgctcca tgagcgccgg ggcaaagtgg
    11941 tggaccgcaa caccctcgcc gacgaggtct ggggtgtgga ttacttcccg gaaagccgcg
    12001 cgctggatca gcacatctcc caactgcgca agcgcatcga ggccgacccc gccagcccgc
    12061 gcatcatccg caccgtccac ggcgccggat accgctacga gtaagtcttc ccatccgttc
    12121 ctggcggcgg cgcgccgctg gaaagcgcaa cttttcccat gcctccgtgt ttccacccgc
    12181 cagaaccgcg cttgcaaggg cgcggcgcct ccatgaactt cactgacatg caccgctttc
    12241 tgatcgtctt gatcctgtcc acgctggccg cggatgtttc cgcgcagaca ccgcccccca
    12301 aaccgtcccc tcccaatgac ggaccgggcg gcaaggacaa gcctcctttt cgcggggatg
    12361 gtcccaagga tcgcggcggc gactggcgcg gcggattccc aggcttcggc cgcccgccca
    12421 tgcgccatga cggcttcgat caactgcccg aggatgagcg ccgcaaagtg cgtgaggcgc
    12481 tcgacaaagt ctggcatctg ccggaggtca tctccgcgcg cgacgaggcc atgcgcgcca
    12541 atgaaaaaat gcgcgacacc atccgcgagt gcctgaaaaa gagcgatcca gaggccgccg
    12601 ccatcgtcga gcgccttgag ccgcgcgagc cgtttgactt ccgcaagctg cctcccctgc
    12661 cgcccacaga ctcggaggct tttcccctgg cgctggtgaa ccgcatgggt tccgaactgg
    12721 aggctttctc acgccctgat cggcgtgagg agacgcgcgg cctgcacctg cgcatcatgg
    12781 acaggcccga aatcaaagcc gccctcgacc gtttgaggca gacccgcggc gaggagcgac
    12841 tccaggccat gcatgaactg cgcaaagcct accgggaggc tgtcggcgcg gagttccagg
    12901 ccgcccgcga gcgccgtgcc gccgaaggcg gaggagtgcc gccgaagaag cctgactgac
    12961 ttgccgcgcc gccgcctgga cttgaccatc ccttcctgga gtgttggagt tgtggtgtgc
    13021 tggatttcca acaatccatg acttcgactg ctgatttcac aatggttggt gacgccgggc
    13081 atggcgaaac gtccggctgg aaactcacgc ctgcacagga gtgcggcggt cgtcgcggaa
    13141 gaaggcttcc atgcgctggc ggaggtcggc atagaaggtc tctgccattt cctccagcat
    13201 gcgggctttg ccggtgatgc ccccggcttc caggcgctcg cgctcgacgc gcattttttc
    13261 ctggaaggcc tgctccagtt ccatgaggtg ctcgaggaag gctttggcgg cgcggctgcg
    13321 gtccacgcct tcaatgaggg cgcgctcaag ggggcggttt tgggtgatgt ggaggaaggg
    13381 gctgtccaca agctgcttca tgtagcggtc gaagccgcgc aggaaggcga ggcgctcgcg
    13441 gtcgtatttg gcctcgtcgc agtcgaggag ggtgtcgtag gggccgggtt tggagaagaa
    13501 tttgacgacg agggggatgg tgtccacgag catgaaaagg gcgcagagga cgaggtaggc
    13561 gatgagggcg aagcggccgc cttcggtgcc gttttcaaag aggtgatgaa gggcgagggt
    13621 ctgggtcagg atgtcgcgcc gtggctcggc gcggatgccg gcgatgcgct cctgctcgtc
    13681 ggcggagagg cgggcgatgt cgtcctggag gcccttcatc tgcatgagga gggcgtctat
    13741 ctgggacttc aaggtctcgc ggatggcgtt ttgctggccg acgaactggt cggcctgctc
    13801 ctgctggacc tggcgcttga gggcgtcgag ccggtc
//
LOCUS       DAOMYJ010000136        13801 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Prosthecobacter sp. isolate AMR_MDS_5629
            NODE_1488_length_13801_cov_20.520151, whole genome shotgun
            sequence.
ACCESSION   DAOMYJ010000136 DAOMYJ010000000
VERSION     DAOMYJ010000136.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867850
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prosthecobacter sp. (wastewater metagenome)
  ORGANISM  Prosthecobacter sp.
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Verrucomicrobiaceae; Prosthecobacter.
REFERENCE   1  (bases 1 to 13801)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13801)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.30
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:34:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,742
            CDSs (total)                      :: 4,684
            Genes (coding)                    :: 4,676
            CDSs (with protein)               :: 4,676
            Genes (RNA)                       :: 58
            tRNAs                             :: 55
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13801
                     /organism="Prosthecobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1488_length_13801_cov_20.520151"
                     /isolate="AMR_MDS_5629"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1965333"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..182)
                     /locus_tag="PLP58_14050"
     CDS             complement(<1..182)
                     /locus_tag="PLP58_14050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ09697.1"
                     /translation="MPDLLPIFEIREAVVSALSDAATPNMLIKAPTGSGKSTQVPQFV
                     LDSGLLREGRRCVVLQP"
     gene            453..1289
                     /locus_tag="PLP58_14055"
     CDS             453..1289
                     /locus_tag="PLP58_14055"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF016041.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sterol desaturase family protein"
                     /protein_id="HRJ09698.1"
                     /translation="MELFREQTLLLISTPLILAAILLEIFVTHFRGLRAYTWRETLTN
                     VYLASLNGGLDLLLRGVLVLPVLAWCHSQSAFEWQRGWIYWLVLFVLEDLAYYALHYA
                     DHHCRIFWAVHVTHHSSEEFNLSTGFRSSVFQPVYRTLYFAPLALLGFQPLDILLMYA
                     ATQIYGSLIHTERIGRLGWLEHVLVTPSHHRVHHARNGPYLDKNMGMCLILWDKIFGT
                     FQEELPQEKPLYGLTKRLENRGPVNIVFHEWRDIWRDLKTSPLPWRRRIGHLLRGPGW
                     RP"
     gene            complement(1334..2587)
                     /locus_tag="PLP58_14060"
     CDS             complement(1334..2587)
                     /locus_tag="PLP58_14060"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HRJ09699.1"
                     /translation="MPEPATLLILALLGAVLWAWRREKSWRRSVMEVTRAAGLETFEA
                     GRLAARFTGLIEAESALAKEEYLRRLFESLLNEIRQGVAIVDDHQRVKFCNRSLGHLM
                     HKSSIRRGRTLLEELGDHQISALVGEALAGEKRRVKEIEHQSISSVGAVSVRHYLVEA
                     APLPAKAESGAWLMIYDITEQTMTEQIRKDFVANASHELRTPLTLINGYIESMQSGVV
                     KDEAGRARCLSVMERHGKRILRIIEDMLTISRLENGPAALNIEPFNVRACVQDALDHL
                     APMIEGRGGTRIELDFPPDGGTLHGDRFYWDQVFTNLLENSLKENPNPGLHIRVQGKW
                     SDGHCTLKVTDNGIGIPAHDLPFVFKRFYRGHKHHSPEIKGTGLGLSIVRRTVEAHGG
                     IIELESTPGIETAFTMKVPLHAAAA"
     gene            complement(2672..3382)
                     /locus_tag="PLP58_14065"
     CDS             complement(2672..3382)
                     /locus_tag="PLP58_14065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018970863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HRJ09700.1"
                     /translation="MSKILVVDDEPDISELITLHLMREGHECVCITNGLQVLNAVIEQ
                     EPDLVVLDLMLPGQDGMSVFKRLRADSRTRGVPVIMLTARAQVTDKINGLELGADDYL
                     TKPFSPRELALRITAILRRTKKVTHSSEVRTGPFLLDRKNMKCFHNDQPVDLTTTEFK
                     LLAVLMENDAAVHTRAELLREVWGYSDDVATRTLDTHIKRLREKLGDAGRHIITVRGT
                     GYQFLADPSAQEAAHVRA"
     gene            3557..4570
                     /locus_tag="PLP58_14070"
     CDS             3557..4570
                     /locus_tag="PLP58_14070"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HRJ09701.1"
                     /translation="MRLLLITDTYPPDINGVARTLSTLARGLAARGHVVEVVTTLAEE
                     PGAASGSPGRHVMASMPLPGYPGLRIGFATTWQMQALFASFMPDALYVATETPLGIAS
                     IRAARKLGIPVVSGFHTNFQTYLEDYALPGLESVAQGLLRALHNQTARTLTPSADTAE
                     MLEKWGVKNVGVLGRGVDTELFHPARRSADLRREWGAGEDAPVAVHVGRVAAEKNLDL
                     LIRAFAAFREEQPGAPCVVVGDGPKLKWMQSAHPGILYAGTRTGEDLARHYASADVFI
                     FPSTTETFGNVVLEALASGVPPVAFDYAGPRQLVKPGVNGWLAPFADDEAFLNQTRAA
                     TRA"
     gene            4731..5606
                     /locus_tag="PLP58_14075"
     CDS             4731..5606
                     /locus_tag="PLP58_14075"
                     /EC_number="3.6.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009959193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008758 - UDP-2,3-diacylglucosamine
                     hydrolase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-2,3-diacylglucosamine diphosphatase"
                     /protein_id="HRJ09702.1"
                     /translation="MSEELEDENGHGPDTKKTKLRARTVFISDVHLGMPDCKAAQVSH
                     FIRNTRCDKLVLNGDIIDVWHLRRLGGWNKSHTHFIRTVLRKMEKENTQIIYLRGNHD
                     DLLDRFIPISLDNFLITDEHIHKTSGGDYLVVHGDGFDHVTTNHPWIAKLGGIGYNIL
                     LRVNRLFNWWRRVRGKESFSLSRWVKLKVKSAVSFVGRYEEQLQELARAKGCKGIICG
                     HIHSPANKIIGDTHYLNSGDWVESLTAIIEHDSGEFEVITYDQFCERTSREPKGGADV
                     AVVAGQADGKAPPPM"
     gene            5612..6313
                     /gene="phoU"
                     /locus_tag="PLP58_14080"
     CDS             5612..6313
                     /gene="phoU"
                     /locus_tag="PLP58_14080"
                     /inference="COORDINATES: protein motif:HMM:TIGR02135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0051049 - regulation of transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HRJ09703.1"
                     /translation="MQDHILSSFDQDLGSLREQVLIMASSARKNLACAMRGLMERDTD
                     LCNAAIAADQDVNELEKSIDRLGMQILLKFQPTAHDLRQVMGTIRIANNLERISDQAG
                     SIAKRARAINLLPEMPEISLIQPVYHLCARNLEAGLQAFIDGDVESAALIRQKDKELD
                     AAEKNLDRALIQVMEARHSPIEGYLHLIFIARFLERIGDHCKNICEDTIFIEKAEDVR
                     FDKTKRAETAAPASW"
     gene            complement(6411..6782)
                     /gene="rplT"
                     /locus_tag="PLP58_14085"
     CDS             complement(6411..6782)
                     /gene="rplT"
                     /locus_tag="PLP58_14085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009960241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HRJ09704.1"
                     /translation="MPRATNAPASRKRRKRTLAKAKGFRGFRSTHFRYAKDAVRKAMT
                     YAYRDRKVRKRTFRNLWIQRINAAARAAGITYSRMMEGLKHAGIEIDRKVLADIAVTD
                     ESAFAALAAQAKAALEKKAAS"
     gene            complement(6905..7267)
                     /gene="rpmI"
                     /locus_tag="PLP58_14090"
     CDS             complement(6905..7267)
                     /gene="rpmI"
                     /locus_tag="PLP58_14090"
                     /inference="COORDINATES: protein motif:HMM:TIGR00001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HRJ09705.1"
                     /translation="MPCQLFFAESAGLSWLPDACRPRSRRVRTRREYAQKPLAVGGIA
                     CIVPAPMSKNSGLSKTRKAVSKRFKVTATGKVLRRKQGKRHILQNKSRKRKRNLGKVA
                     LVAEVDKANVLASMPYSR"
     gene            7301..7375
                     /locus_tag="PLP58_14095"
     tRNA            7301..7375
                     /locus_tag="PLP58_14095"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:7333..7335,aa:Val,seq:cac)
     gene            7445..8845
                     /locus_tag="PLP58_14100"
     CDS             7445..8845
                     /locus_tag="PLP58_14100"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase"
                     /protein_id="HRJ09706.1"
                     /translation="MSPRFAALLFLLPLPCSATPVENVLFIVSDDLRASVAGCYGDAV
                     CATPNMDRLAARGVVFERAYCQGTVCGPSRASFMRGRYYGGPGVTLGEHFIAQGRTTA
                     RVGKIFHMRVPGDIIAGTDGEDVPACWSERHNAQGMEAHTPGDYACLNLNIFTTELEG
                     RQSTGDPHRPFVTVSYEGDGSDQPDAKAAAKSVELLRKFSAEKKPFLLATGFVRPHYP
                     MVAPRPYFDRYPHAKIELPHVPEKDLDDIPPSGISSSNSEKNGLAKYSDNQRRMWSGY
                     YAAVTFMDAQLGRILDEVDRLGLAESTAIVFTSDHGYHLGDHTFWQKANLHEQVTRVP
                     LIIAAPGVRPGRSRSLAQLVDIFPTLCELTGAPVPEGVQGKSLVPVLHDNTARIHEAA
                     FSYDKGHHALRSDRWAYMRYTDGSEELYDMEQDPGQFTNLAGQAGVSAVVEKLRGQLQ
                     AYAPPSAGPKRKKKQK"
     gene            8870..10750
                     /locus_tag="PLP58_14105"
     CDS             8870..10750
                     /locus_tag="PLP58_14105"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HRJ09707.1"
                     /translation="MTRLHAVLLAAAMPAHAEPPRPNILWLTAEDMSPNLGCYGDADA
                     ITPRLDAFAKEAVRYDRAFATAPVCSPARSCLITGMFATSLGTQRLRSAFPIPAEFGP
                     FTERLREAGWHCVNNVKTDYNLRDEPAFIRRAWEESSPTAHWRSRREGQPFFAVFNFM
                     TTHQSRTSVWPHEEFEREIGSKLSPGERHDAAHVTLPPFYPDTAEARRAWARYHDCVT
                     RMDTQAGEILDQLEADGLAEDTIVFFFADHGMGMPRGKRCLHDSGLRVPLLVRFPKKW
                     AQLAPGAPGSATERLVSFVDFAPTVLSLCGVKAPAHFQGRAFLGADAAPPREFVHGAR
                     DRVDEAFDLSRSVRDGRWLYIRNFMPHLSWMQPEGYSDASTFRQELKHLAAQGRLEGG
                     AGFYAAPSRSLEELYDTRADPHQLRNLAHTPEHETTLARMRAGLRRWQMETRDAGFLT
                     EPQMWERLARDETPWQIARDDSRYPLARLLEAADAVGRKDAQERQRQWLRDPHDAVRY
                     WAVTGLFARETLTKADIQALREVLQDSSTIIRVEAASALARQGETGEALPVLTAALRS
                     ESVETRLHAARALELAGAVAIPTREAMKSALDAAREAEKSGDTFSMFIRFSLEAALRR
                     ED"
     gene            10797..11366
                     /locus_tag="PLP58_14110"
     CDS             10797..11366
                     /locus_tag="PLP58_14110"
                     /inference="COORDINATES: protein motif:HMM:NF012787.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin domain-containing protein"
                     /protein_id="HRJ09708.1"
                     /translation="MKLPAIIFFLLCSLPSTPAHAQELTLPDCDSKPHAPLDSGGKAA
                     VVLFFVSPFCPTTKSFMAEINQISGDHAEKARFFLIHSDPAVTPEVAFQHAEMSGVRM
                     TVLLDTEQKLARHAGARVTPETVVFSPQGAVLYRGRVNDLYLGPTKRQRAATTRDLRD
                     ALEAVFAGRPVPEPQHEAQGCKISGIPQP"
     gene            complement(11415..>13801)
                     /locus_tag="PLP58_14115"
     CDS             complement(11415..>13801)
                     /locus_tag="PLP58_14115"
                     /inference="COORDINATES: protein motif:HMM:NF012485.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="secretin N-terminal domain-containing protein"
                     /protein_id="HRJ09709.1"
                     /translation="ASADPAEREAIRPEDAIKPGGGVELQFPNTPLSQILLVYEDLTG
                     LKIIRDANAEQATVSIETTGELPKDKAIMFIEKSLLLNGYSFVPAGEGMVKLLSEAKK
                     PQTEGAPFIESAFDLPETDQVVNHVVILKHLNTEDAQKAIDQVIPRHSYGLVHPVPNA
                     KALVITENSNTIRSILALLERIDNKPAPTISRTIQLTRSDAEDVKKALEEILGLSESG
                     SNSSGGSRGSGARPAVPNPGAAQMGIVPQGETPQAASASAAGGGTASSTEETPPKIHA
                     IVRRNCLLVMASPETMETIQTLVDELDAASDLRNFVSRTLKYLGVEAALGIISDAISR
                     TEGGENGGAGGGVNSLGQSGAGATNTQTNSGGLFGNNNSNTGGGGLFGNNNNSGGFGS
                     GLGGGLGGGGFGGGGSGFGGGGGNLQPLRPNNGPRSLVIGKTLLISDPVANSVFASGP
                     PEHLRILNEILDELDRRPQQILISAVIGEAQYGKEGSIGIEYLIRARERQGSGFTRGL
                     AGQLGQAAVLPLDPRTAVPLADLALRSGLTFYGGVREGLDLIVHTLQTSSDFKVISRP
                     SVFTMNNTAANISSGSSFPIATSTQGLIGGGTTGTGLLSNVQYQDVVLSLNIVPLINS
                     DDELTLQISQENSEVAGSTVIAENTYPILTKQQLNTVVMCKNNSTVLLGGLIRESDED
                     EKTNVPILGSIPFIKHLIGTTRKEDNRRELLIMLQPSIIEGMHDIPPGVRHAPGSSPF
                     GEETTRFISQEKSPVYEQVPVERRNRVMELARKLFKRPQNAQTEAAVVRRPGAR"
BASE COUNT         2359 a         4326 c         4583 g         2533 t
ORIGIN      
        1 ggctgcagca ccacacagcg ccgcccctcc cgcagcaggc ccgagtcgag cacgaactgc
       61 ggcacctggg tggacttgcc cgagccggtc ggcgccttga tcagcatgtt cggcgtggcc
      121 gcgtcactca gcgcggagac gacggcttca cggatttcaa agatgggcag caggtcgggc
      181 atgaggcgcg cagttcatgc gcgcggagga cgggcttgtc aaaacggggg atttcggaat
      241 gcaatgccaa gccagtcaga agccattctt gggcggcccg aatgccaaat gaatcattca
      301 attgcggcac cggctgttgc cggggaacga agacaaggcg ggctttgagc ctgccgctca
      361 cggtgcattt gagacgggga aaccggtgct tttctggaca tcgccctcat ttgtggcaca
      421 cctgatgccc tccaccgacc gccccctgcc tgatggaact tttccgcgaa cagactctcc
      481 tgctgatcag cacaccgctg atcctcgccg ccattctgct ggagatattc gtcacccact
      541 tccgcggcct gcgcgcctac acctggcgcg agacgctgac gaatgtctat ctggcgtccc
      601 tcaacggcgg gctggacctg ctgctgcgcg gagtgctcgt gctgcccgtg ctggcttggt
      661 gccacagtca aagcgccttt gagtggcagc gtggctggat atactggctg gttttgttcg
      721 tgctggagga cctggcctat tacgcgctgc actacgcgga ccaccactgc cggatttttt
      781 gggcggtgca tgtcacgcac cactcctcag aggagtttaa tctcagcacc ggcttccgtt
      841 cctcggtctt ccagccggtc tatcgcacgc tttacttcgc gccgctggcg cttctcggct
      901 tccagccgct ggacatcctg ctcatgtatg ccgccacgca gatttacgga agcctgatcc
      961 acaccgagag gatcggcaga ctgggctggc tggagcatgt gctggtgaca cccagccacc
     1021 accgcgtcca ccacgcccgc aacggccctt atctcgacaa aaacatgggc atgtgcctca
     1081 tcctctggga caagatcttc ggcacattcc aggaggagct gccgcaggaa aagccgctct
     1141 acggcctgac caaaaggctc gaaaaccgcg gccctgtgaa cattgtcttc cacgagtggc
     1201 gggacatctg gcgcgatctg aaaaccagcc ccctgccctg gcgccggcgc atcggccacc
     1261 tgctgcgcgg ccccggctgg cggccttgag cagaacttcc gttttttccc aatctggcca
     1321 gtcggcggag gaatcatgcg gcggcggcgt ggagcggcac tttcatggtg aaggcggtct
     1381 cgatgcctgg agtgctctcc aactcgatga tgccgccatg cgcctcaacg gtgcggcgga
     1441 cgatgctgag gccaaggccg gtgcccttga tctccgggga gtgatgcttg tggccgcggt
     1501 agaagcgctt gaaaacgaag ggcaggtcgt gcgcggggat gccgatgccg ttgtcggtga
     1561 ctttgagcgt gcagtgaccg tcgctccatt tgccctggac tcggatgtga aggccggggt
     1621 tgggattctc tttgagggag ttttcaagga ggttggtgaa gacctggtcc cagtaaaagc
     1681 ggtcgccatg aagggtgccg ccgtcggggg ggaagtcgag ttcgatgcgc gtgccgccgc
     1741 ggccttcgat catgggggcg agatggtcga gggcgtcctg gacgcaggcg cggacgttga
     1801 atggctcgat gttgagggcg gcggggccgt tttccaggcg ggagatggtg agcatgtcct
     1861 cgatgatgcg cagaatgcgc ttgccgtgcc tttccatgac ggaaaggcag cgggcgcgtc
     1921 cggcctcgtc cttgacgaca ccgctctgca tggactcgat gtagccgttg atgagggtga
     1981 ggggggtgcg cagctcgtgg gaggcgttgg cgacgaagtc cttgcgtatc tgctcggtca
     2041 tcgtctgctc ggtgatgtcg taaatcatga gccaggcgcc gctctcggcc ttggcgggca
     2101 ggggggcggc ctcgacgagg tagtggcgga cgctgacggc gccgacactt gagatgctct
     2161 ggtgctcgat ctctttaacg cggcgttttt ccccggccag ggcctcgccg acgagggcgc
     2221 tgatctggtg gtcgcccagc tcctcaagca gggtgcggcc gcggcggatg gagcttttgt
     2281 gcatgaggtg gccgaggctg cggttgcaga atttcacgcg ctggtggtcg tccacgatgg
     2341 cgactccctg gcggatttca ttgagcaggg actcgaagag gcggcggaga tactcctctt
     2401 tggcgagggc ggactcggcc tcgatgaggc cggtgaagcg ggcggcgagc ctgccggcct
     2461 cgaaggtctc cagcccggcg gcgcgggtga cttccatgac gctgcgcctc cagctttttt
     2521 cacgccgcca tgcccagagc accgcgccga ggagcgcgag gatgagcagt gtggcgggtt
     2581 ctggcatggg cggtgctggc cgtggaaagt ctggccgccg gcgctttttt caaggcggga
     2641 tgatccgcgc ggcggcgcgc ttttttcacc ttcatgcacg gacgtgggcc gcctcctggg
     2701 cggacggatc agccaggaac tggtatccgg tgccgcggac ggtgatgatg tgacggccgg
     2761 cgtcgccgag tttttcgcgc aggcgcttga tgtgggtgtc gagcgtgcgg gtggccacat
     2821 cgtcggagta gccccagacc tcccgcaaaa gctcggcgcg ggtgtggacg gcggcgtcgt
     2881 tttccatgag cacggccagg agcttgaact cggtggtggt gagatccaca ggctggtcgt
     2941 tgtggaagca cttcatgttc ttgcggtcga gcaggaaggg gccagtgcgg acctcggagc
     3001 tgtgggtgac ttttttggtg cggcgcagga tggcggtgat gcgcagggcg agctcgcgcg
     3061 gggagaaggg cttggtgagg tagtcgtcgg cgccgagttc gaggccgttg atcttgtcgg
     3121 tgacctgggc tcgggcggtg agcatgatga cgggcacgcc gcgggtgcgg ctgtcggcgc
     3181 ggaggcgctt gaagacggac atgccgtcct ggccggggag catgaggtcg agcacgacga
     3241 ggtcgggctc ctgctcgatg acggcgttga ggacctgcag gccgttggtg atgcagacgc
     3301 actcatggcc ttcacgcatg aggtgaaggg tgatcagctc cgaaatgtcg ggttcgtcat
     3361 ctacgaccag gattttgctc ataaggacgc tccgagtaca ctggcggcag ggctgtcaac
     3421 aaacataaat tggtgacgac tccgacacat gctaccctga aaccagcgcc gggaaagcgt
     3481 gtcgtgacac attcgtcaca atgcggagct ttgtcggggc gtgtttctgc tctctgtaac
     3541 tcattccgcg cccgccatga ggctcctgct catcaccgac acctacccgc cagacatcaa
     3601 cggggtggcg cgcactctct ccaccctggc gcgcgggctc gcggcgcgcg ggcatgtggt
     3661 ggaggtggtg acgacgctgg cggaggagcc tggcgcggcg tccgggtctc ccggccggca
     3721 tgtgatggcg tccatgccgc tgccgggtta tccggggctg cgcatcggct tcgcgacgac
     3781 atggcagatg caggcgcttt ttgccagctt catgccggac gcgctgtatg tggccacgga
     3841 gacgccgctg ggcatcgcca gcatccgcgc ggcgaggaag ctgggcatcc cggtggtgtc
     3901 aggcttccac acgaacttcc agacctacct cgaggactac gcgctgccgg ggctggaaag
     3961 cgtggctcag ggcctgctgc gcgcgctgca caaccagacg gcgcgcacgc tgacgccaag
     4021 cgcggacacg gcggagatgc tggagaagtg gggcgtgaaa aacgtgggcg tgctggggcg
     4081 cggcgtggac acggagcttt tccaccccgc gcgacgcagc gcggacctgc gccgcgagtg
     4141 gggagcgggg gaggatgcgc cggtggcggt gcatgtgggg cgggtggcgg cggaaaagaa
     4201 tctggatctg ctcatccgcg ccttcgccgc cttccgcgag gagcaacccg gcgcgccatg
     4261 cgtggtggtg ggggacgggc cgaagctgaa gtggatgcag tcggcgcatc caggcatcct
     4321 ctatgcggga acgcgcacgg gggaggacct ggcgcggcac tacgccagcg cggatgtgtt
     4381 tatcttcccc agcacgacgg agacgtttgg caatgtggtg ctggaggcgc tggcctccgg
     4441 ggtgccgccg gtggcgtttg actatgcggg gccgcgccaa ctggtgaagc ctggagtaaa
     4501 tggctggctg gcacccttcg ctgatgatga ggcttttttg aaccagaccc gcgccgcgac
     4561 ccgcgcgtag aatgccgcgc cgatgcatga ggccgcgcgc gccgcggcgc tggagctggg
     4621 ctggcagcgt gtcattgagc agtttgagca ggaactgctg agcgcgcccc ccgccggacc
     4681 ggctgccagt gcttgacgcg catgattttc accacccttt gaccgccatc atgtccgaag
     4741 aactcgaaga cgaaaacggc cacgggccgg acacgaagaa aaccaagctg cgcgcgcgca
     4801 cggtcttcat ctcggacgtg catcttggca tgcccgactg caaagcggcg caggtctcgc
     4861 atttcatccg caacacgcgc tgcgacaagc tggtgctcaa cggcgacatc attgatgtgt
     4921 ggcacctgcg ccggctcggc gggtggaaca aaagccacac gcacttcatc cgcaccgtcc
     4981 tgcgcaagat ggagaaggag aacacgcaga tcatctacct gcgcggcaac cacgacgacc
     5041 tgctggaccg cttcatcccc atctcgctcg acaacttcct catcaccgac gagcacatcc
     5101 acaaaacttc cggcggcgac tatctcgtgg tccacggcga cggcttcgac catgtgacca
     5161 cgaaccaccc ctggatcgcg aagctgggcg gcatcggcta caacatcctc ctgcgcgtca
     5221 acaggctgtt caactggtgg cggcgggtgc gcggcaagga gagcttttcc ctcagccgct
     5281 gggtgaagct gaaggtgaag tcggcggtga gcttcgtcgg ccgctatgag gagcagctcc
     5341 aggagctggc cagggccaaa ggctgcaaag gcatcatctg cggccacatc cacagccccg
     5401 cgaacaagat catcggcgac acccattatc tgaattccgg cgactgggtg gagtcgctca
     5461 ccgccatcat cgagcatgac agcggcgagt tcgaagtcat cacctacgac cagttctgcg
     5521 agcgcaccag ccgcgagccg aaaggcggcg ccgacgtggc cgtcgtcgcc ggccaggcgg
     5581 acggcaaagc cccgccaccc atgtgacccc catgcaagac cacatcctgt ccagttttga
     5641 ccaagacctg ggaagcctgc gcgagcaggt gctcatcatg gccagcagcg cgcgcaaaaa
     5701 cctggcctgc gccatgcgcg gcctgatgga gcgcgacacc gacctgtgca acgcggccat
     5761 cgccgccgac caggatgtca acgagctgga gaagagcatt gaccgcctgg gcatgcagat
     5821 cctgctgaaa ttccagccca ccgcgcacga cctgcgccag gtcatgggca cgatccgcat
     5881 cgccaacaac ctggagcgca tctcggacca ggcgggcagc atcgccaagc gcgcgcgcgc
     5941 catcaacctg ctgccggaaa tgcccgagat cagtctcatc cagcccgtgt atcacctctg
     6001 cgcgcgcaat ctggaggccg gcctccaggc gttcattgac ggggatgtcg agtccgcggc
     6061 tctcatccgc cagaaggaca aggagctgga cgcggcggaa aagaacctgg atcgcgcgct
     6121 catccaggtc atggaggcgc gccattcgcc gatcgagggt tacctgcacc tcatcttcat
     6181 cgcgcgcttt ctggagcgca tcggcgacca ctgcaaaaac atctgcgagg acaccatttt
     6241 catcgagaaa gcggaggatg tgcgctttga caagacgaag cgcgccgaga ccgccgctcc
     6301 cgcgtcgtgg tgactcgagc gcgggcgctc gtcaaacaaa cgggctgcga aggcaaaaaa
     6361 aacgccgccc ttgcgggcgg cggttgatcc aggaagccag gggggatgaa tcaggaggcg
     6421 gcctttttct ccagcgcggc cttggcctgg gcggccaggg cggcaaaggc ggactcgtca
     6481 gtcacggcaa tgtcggcgag aaccttgcgg tcaatctcga tgccggcgtg cttgagaccc
     6541 tccatcatgc ggctgtaggt gatgccggcg gcgcgggccg cggcgttgat gcgctgaatc
     6601 cacaggttgc ggaaggtgcg cttgcggacc ttgcggtcac ggtaggcata cgtcatcgcc
     6661 ttgcggacgg cgtccttggc gtaacggaag tgggtggaac ggaagccgcg gaatcccttg
     6721 gctttggcga gtgtgcgctt gcgccgcttg cggctggcgg gtgcgttggt ggctctgggc
     6781 atgtgtgtgt ttctgcgagc aggctgttgt tggagattcg atgaccggcc tcacctgttc
     6841 atagaccccg cggggcgggg ccagaccgga gcatcgtggc ccccggcggg ggccgtggag
     6901 caggtcagcg cgagtaaggc atgctcgcga ggacgtttgc tttgtccact tccgccacga
     6961 gggcgacttt gccgagattg cgtttccgtt tgcggctctt gttttgcagg atgtggcgct
     7021 tgccttgttt gcggcgcagc accttgcccg tcgcggttac tttgaaccgt ttggagacag
     7081 cttttctcgt tttagacaaa cctgagtttt tggacatagg ggcggggaca atgcaggcga
     7141 tccccccgac ggcaagcggt ttttgggcat attcacggcg cgttctcacc ctgcgcgacc
     7201 gcggacggca agcgtccggc aaccaggaaa gcccggcact ttccgcaaaa aacaactggc
     7261 agggcaaggg gcggcatgta ttctcggccc ctctccaagg gcgcgcttag ctcagtggta
     7321 gagcactgcc ttcacacggc aggtgtcgca ggttcgaacc ctgcagcgcg caccattttt
     7381 ccggcaaaaa gccggcctcc acgcagcctt ggaagaagca cgccgcgcga gcgttccagt
     7441 cgccatgtcc cccaggttcg ccgcgcttct tttcctgctt cccctgccct gctccgccac
     7501 accggtcgaa aacgtgctct tcatcgtctc cgacgacctg cgcgccagcg tggccggctg
     7561 ctacggcgac gccgtgtgcg cgacgccgaa catggaccgc ctggcggcgc gcggcgtggt
     7621 ctttgagcgc gcctactgcc agggcacggt gtgcgggccc tcgcgcgctt ccttcatgcg
     7681 cgggcgttac tatggcgggc cgggtgtcac gctgggagag catttcatcg cccaggggcg
     7741 gacgacggcg cgggtgggga agattttcca catgcgcgtg ccgggtgaca tcatcgccgg
     7801 cacggatggc gaggatgtgc ccgcgtgctg gtcggagcgg cacaatgccc aaggcatgga
     7861 ggcgcacacg ccgggtgact acgcctgcct gaacttgaac atcttcacca ccgaactcga
     7921 aggccgccag tccacgggtg atccgcaccg gcccttcgtc accgtcagct atgaggggga
     7981 cggctccgac cagccggatg caaaggcggc ggcgaagtcc gtggagctgc tgcgcaagtt
     8041 cagcgcggag aagaagccct tcctgctcgc cacgggtttc gtgcgcccgc attaccccat
     8101 ggtggcgccg cgtccgtatt tcgaccgcta cccgcacgcg aaaatcgagc tgcctcatgt
     8161 gccggagaag gatctcgacg acatcccgcc gtccggcatc tccagctcga actcggagaa
     8221 aaacggcctg gccaaatatt cggacaacca gcgccgcatg tggtccggct attatgcggc
     8281 ggtgactttc atggacgcgc agctcgggcg catcctcgac gaggtggacc gcctcggcct
     8341 ggcggagagc acggccatcg tcttcaccag cgaccacggc taccacctgg gcgaccacac
     8401 cttctggcaa aaggccaacc tgcacgagca ggtcacgcgc gtgccgctca tcatcgccgc
     8461 gccgggcgtg cggccgggca ggagccgctc cctcgcgcag ttggtggaca ttttccccac
     8521 gctctgcgag ctgaccggcg cgcccgtgcc ggagggagtg caggggaaaa gcctcgtgcc
     8581 ggtgctgcac gacaatacgg cccgcattca cgaggccgcc ttctcgtatg acaaaggcca
     8641 ccacgccttg cgctcggacc gctgggccta catgcgctac acggacggct ccgaggagct
     8701 gtatgacatg gagcaggacc ccggccagtt caccaacctc gccgggcagg cgggcgtgag
     8761 cgcggtggtc gagaaattac gcggacaatt gcaggcgtat gcgccacctt cagccggacc
     8821 gaaacgcaag aagaagcaaa agtaacgccg taaccaagcg atgcgttttt tgacccgact
     8881 tcatgccgtg ctgctggcag cggcgatgcc ggctcacgcg gagccgccgc gcccgaacat
     8941 cctctggcta accgccgagg acatgagccc gaatctcggc tgctacggtg atgcggacgc
     9001 catcacgccg cgcctcgacg ctttcgccaa agaggccgtg cgttacgacc gcgccttcgc
     9061 caccgcgccg gtgtgctcgc ccgcgcgctc ctgcctgatc accggcatgt ttgccaccag
     9121 cctcggcacg cagcggctgc gctcggcttt tccgattccc gcggagttcg gccccttcac
     9181 cgagcgactg cgcgaggcgg gctggcattg cgtgaacaac gtgaagaccg attacaattt
     9241 gcgcgatgag cccgccttca tccgccgcgc ctgggaagaa agctcgccca cggcgcactg
     9301 gcgctcgcgg cgcgaagggc agccgttttt tgccgtcttc aacttcatga ccacgcacca
     9361 gtcccgcacc agcgtgtggc cgcacgagga gttcgagcgt gagatcggct ccaagctctc
     9421 gccgggcgag cggcatgatg ccgcgcatgt cacgctgccg ccgttttacc cggacacggc
     9481 ggaggcccgc cgcgcctggg cccgctacca cgactgcgtc acgcgcatgg acacgcaggc
     9541 gggggagatt ctcgaccagc tcgaagccga cggactcgcg gaggacacga tcgtgttttt
     9601 cttcgccgac cacggcatgg gcatgccgcg cgggaagcgc tgccttcacg actccggcct
     9661 gcgcgtgccg ctgctcgtcc gttttccgaa aaagtgggcg cagctggcgc cgggcgcgcc
     9721 cggctcggcg acggagcgcc tggtgagctt cgtggacttc gcacccaccg tgctcagcct
     9781 gtgcggagtc aaagcgcccg cgcatttcca gggccgcgcc ttcctcggcg cggatgccgc
     9841 gccgccgcgc gagttcgtgc atggcgcgcg cgaccgcgtg gatgaggcct tcgatctctc
     9901 ccgcagcgtg cgcgacgggc gctggctcta cattcgcaac ttcatgccgc acctttcctg
     9961 gatgcagccc gagggttact ccgacgcgtc caccttccgc caggagctga aacacctcgc
    10021 cgcgcagggc cggctggagg gcggcgcggg attttatgcc gcgccgtccc gatctctgga
    10081 ggagctctat gacacacggg ccgatccaca ccaactccgc aacctcgccc acacgccgga
    10141 gcatgagacg acactggcgc ggatgcgcgc cgggctgcgc cgctggcaga tggagacgcg
    10201 ggacgcgggc ttcctcaccg aaccgcaaat gtgggagcgt cttgcccggg atgaaacgcc
    10261 ctggcaaatc gcgcgcgatg actcgcggta tcctcttgcc cgcctgctcg aagcggcgga
    10321 tgccgtcggg cgcaaggatg cccaggagcg ccagcgccag tggttgcgcg accctcatga
    10381 cgccgtgcgc tactgggccg tgaccggcct tttcgcccgc gagactttga caaaggcgga
    10441 catccaggcg ttacgcgagg ttttgcagga ctcatccacc atcatccgtg tcgaagccgc
    10501 gtccgccctg gcgcggcaag gcgagaccgg ggaggcattg ccggttctca ccgccgctct
    10561 gcgctccgaa tccgtggaaa caaggcttca cgcggcccgc gcacttgaac tcgccggtgc
    10621 tgtcgccatt cccacgcgcg aagcgatgaa gagcgccctt gatgcggcgc gcgaggccga
    10681 gaaatccggc gacacattct ccatgttcat ccgcttcagc ctcgaagccg cgctgcgtcg
    10741 cgaggattga agagtttgcg caaacggcat cctcgcgctc ccgtgcgcgc ctccgcatga
    10801 aactgcccgc catcatcttc tttctgcttt gctccctgcc ctccacaccg gctcacgcgc
    10861 aggaactgac gctgcccgac tgcgactcca aaccgcacgc gccgctggat tccggcggca
    10921 aggcggccgt ggtgctgttt ttcgtctcac ccttctgccc gacgacgaaa tccttcatgg
    10981 ccgagatcaa ccaaatctcc ggcgaccatg cggagaaggc gcgctttttc ctcatccact
    11041 cagaccccgc cgtcacgccc gaagtggcgt tccagcacgc ggagatgtcg ggtgtgagga
    11101 tgacggtgct gctcgacacg gagcaaaagc tggcccgtca tgccggcgcg cgcgtcacac
    11161 ccgagacggt ggtcttctcg ccgcaggggg cggtgctcta tcgcggccga gtcaatgacc
    11221 tatacctcgg ccccaccaag cgccagcgcg cggccaccac gcgggatttg cgagacgcgc
    11281 ttgaggccgt ctttgcaggg aggcccgtgc ctgagccgca gcacgaggcg caaggctgca
    11341 aaatcagcgg aatcccgcag ccgtgagatt ttcctgcgcg ccttcccgcg gacaatgttc
    11401 acctcgccac attattatct ggcccccgga cggcgcacga cggccgcctc ggtttgagcg
    11461 ttctgggggc gcttgaaaag tttgcgcgcg agttccatga ctcggttgcg gcgttcgaca
    11521 ggcacctgct cgtaaaccgg gcttttctcc tgcgagatga aacgcgtggt ctcctcaccg
    11581 aacggactgc tgccaggcgc gtggcgcaca ccaggtggga tgtcatgcat gccttcgatg
    11641 atgcttggct gaagcatgat gagcaactca cgccggttgt cctcttttcg agtggtgcca
    11701 ataagatgtt tgatgaacgg aatgctgcca aggatgggca cattggtttt ttcgtcctcg
    11761 tcactctcgc ggatcaagcc gccaaggagc acggtggagt tgtttttgca catgacgact
    11821 gtgttgagct gctgcttggt cagaatgggg taggtgttct cggcgatgac cgtgctgccg
    11881 gccacctcgc tgttctcctg ggagatttgc agggtcaact cgtcgtcgga gttgatgagg
    11941 ggcacgatgt tcaggctgag cacgacatcc tgatactgga cgttggaaag cagtccggtg
    12001 cctgtggtgc cgccgccgat gagaccctgg gtggatgtgg cgatgggaaa cgaggagccg
    12061 ctgctgatgt tggccgccgt gttgttcatg gtgaagacgg agggccgcga gatgaccttg
    12121 aaatcggagc ttgtctgcag cgtgtggacg atcaagtcga ggccttcgcg cactcctccg
    12181 taaaaagtca ggccggagcg cagcgcaaga tccgcaagcg gcacagcagt gcgcgggtcc
    12241 aaagggagca cggctgcctg gccgagctga cctgccaggc cccgggtgaa gcccgaaccc
    12301 tggcgctcgc gagcgcggat gaggtattca atccctatgg agccttcctt gccatactgc
    12361 gcctctccaa tgaccgctga aatgagaatc tgctgcggac ggcggtccaa ttcgtcgaga
    12421 atttcattca gaatgcgcag gtgctcgggc ggcccggagg cgaagacgga gttcgccaca
    12481 ggatcgctga tgagcagcgt tttgccgatg acgagcgagc gcgggccgtt gttggggcgc
    12541 agcggctgaa gattgccgcc gcctccacca aagccactgc ctccaccgcc aaaaccgcct
    12601 ccgccgagtc cgccgcctaa tccactgcca aaacctccgc tgttattatt gttgccaaaa
    12661 aggccgccgc cgccggtgtt gctgttgttg ttgccgaaca agccgccgga gtttgtctgt
    12721 gtgttggtgg cgccggcgcc actctgcccc aggctgttca cgccgcctcc ggcgccgccg
    12781 ttttccccgc cctcggtgcg ggagatggcg tcgctgatga tgccgagcgc tgcttcgacg
    12841 ccaagatatt tgagcgtgcg cgagacaaaa ttcctgaggt cggaagccgc gtcgagctcg
    12901 tccacgagcg tttgaatggt ctccatcgtc tccgggctgg ccatgacgag caggcagttg
    12961 cgccggacga tggcgtggat cttgggcggt gtctcctcgg tggacgaagc cgtgccgccg
    13021 cctgccgctg aggctgacgc ggcctgcgga gtctcgccct gcggcacgat gcccatttgc
    13081 gccgccccag gattcggaac ggcggggcgg gcgcctgagc cgcggctgcc gccactggaa
    13141 ttggagccgc tctcggacag tccaaggatt tcctccagcg cctttttcac atcctccgca
    13201 tccgagcgtg tgagctggat ggtcctggag atggtgggcg cgggtttgtt gtctatgcgc
    13261 tccagcagcg ccaggatgga gcggatggtg ttgctgtttt cggtgatgac cagcgccttg
    13321 gcgttcggca cgggatgcac aagaccgtag ctgtggcggg ggatgacctg gtcaatggcc
    13381 ttctgcgcgt cctcggtgtt gaggtgtttg agaatgacga cgtgattgac cacctggtcg
    13441 gtctccggca gatcgaaggc ggactcgatg aagggcgcgc cctcggtctg gggttttttg
    13501 gcctcggaga gcagcttcac catgccttcg ccggcgggca caaaggagta tccattcaaa
    13561 agcaggctct tctcgatgaa catgatggcc ttgtccttcg gcagctcgcc agtggtctcg
    13621 atggagacgg tggcctgctc ggcattggca tcccggatga tcttcagccc cgtgagatcc
    13681 tcgtaaacga gcaggatctg cgaaagcggt gtgttcggaa actgcagttc cacgccgccg
    13741 ccgggtttga tggcgtcctc gggccggatg gcctcgcgct cggcggggtc ggcgctcgct
    13801 g
//
LOCUS       DAOMYJ010000137        13656 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Prosthecobacter sp. isolate AMR_MDS_5629
            NODE_1507_length_13656_cov_22.040291, whole genome shotgun
            sequence.
ACCESSION   DAOMYJ010000137 DAOMYJ010000000
VERSION     DAOMYJ010000137.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867850
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prosthecobacter sp. (wastewater metagenome)
  ORGANISM  Prosthecobacter sp.
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Verrucomicrobiaceae; Prosthecobacter.
REFERENCE   1  (bases 1 to 13656)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13656)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.30
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:34:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,742
            CDSs (total)                      :: 4,684
            Genes (coding)                    :: 4,676
            CDSs (with protein)               :: 4,676
            Genes (RNA)                       :: 58
            tRNAs                             :: 55
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13656
                     /organism="Prosthecobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1507_length_13656_cov_22.040291"
                     /isolate="AMR_MDS_5629"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1965333"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1119)
                     /locus_tag="PLP58_14120"
     CDS             complement(<1..1119)
                     /locus_tag="PLP58_14120"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HRJ09710.1"
                     /translation="MAVPNSSTFPSLQELSAWLPAFEVLRRLESGRDSAVYLARQTAL
                     ERLVDIRVMRNPGGQEESLMLARLRARAKLVHPNCVAVYDVGKSPAGLWYLISEHIEA
                     RAIAEQIQGREITPKTAFTLALPLCSALQAVHDHGLHHGALSPRTVLITSAGQLKLTG
                     IGMARLETGELTWIHGDTSSKAADIRALGATLHEMFARQPPSPDGRTARELPPAFALV
                     LRRCLETDPARQYTSAAQAGHALLDARQAKPKKEAAAPKAADAPPAKDFSQPAATPPA
                     RRPVPPPPQPIQRQGRTFMQKLDAFLWDGLKVGLHLTVTLVSIGSLVALYLFKDRIVI
                     EERIERPGALPPNAAAQIEEPPIPAAVMGALPAAPTLPV"
     gene            1337..3133
                     /locus_tag="PLP58_14125"
     CDS             1337..3133
                     /locus_tag="PLP58_14125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018289958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004783 - sulfite reductase (NADPH)
                     activity [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent assimilatory sulfite reductase
                     hemoprotein subunit"
                     /protein_id="HRJ09711.1"
                     /translation="MSKASVEDIKLTSAGLRGQLAELFADTSTPNIPEDSNILLKHHG
                     SYQQDDRDRRAALVKAKQEKAWSFMIRSKMPGGRISAKQWMIHDELCTKAFGNMRLTN
                     RQGIQLHGVLKGGLKEVIHNVCHSGLSTMGACGDVVRNTMGPAAPVKDAVHADTQRLT
                     EEISQRFLWRSSAYADIWLDGEKVEPEWIKDPAVNPAVREATQAPEGDDPIYGKVYLP
                     RKFKIGVAIQPCNDVDVFSQDVGFVPHVVEGRVEGYTITVGGGFGMSHGQLATRPFLA
                     QPLLYVKRAHVVDACEAIVTTQRDHGNRAERKHARLKYTVQSMGIEAFRAEVVRRLPG
                     IETFPPKELKFDSVEDTLGWHEQGDGKLYCAVYVSMGRIVNSENGPQYRGAFAEIATR
                     LDLPFIVTPNTNLIIADVAPEQKEQVNAILAKHGVVHADEAGFTRARKVAHACVALPT
                     CGLALSESERALPGFMDQFDAMLQELGIVDEPILFRMTGCPNGCGRPYNADFGFVGRA
                     PNKWAMFVGGSIRGDTLAGLEFKSVTGEEIPGKVRAILEAFIAERQPGEIFADWFART
                     RTKGAEPTSEQFHIELAERAAKLAGEKAGEAG"
     gene            3180..3938
                     /locus_tag="PLP58_14130"
     CDS             3180..3938
                     /locus_tag="PLP58_14130"
                     /inference="COORDINATES: protein motif:HMM:NF014670.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="creatininase family protein"
                     /protein_id="HRJ09712.1"
                     /translation="MQLAEMTWPEVAALPRHTPVVFPVAALEQHGRHMPLFTDSLLMG
                     EIARRTEEELRGSALFAPLQWLGNSHHHLDFPGTLSAGPRVYLDLLCGLLENFIAHGF
                     TRLLILNGHGGNDVPGRQAIFEVRQRHRERKDLLLLFATYWNLAPHAHEAHPGLSQRQ
                     MGHACEWETSMILRLSPHLVKGHAQAVEVPFGCPFEPAARGWTMPDRSAPGHVGDPRA
                     ASAEKGEALFQAFNAATVAMLRRMIAWDGKSWEG"
     gene            complement(3958..5244)
                     /locus_tag="PLP58_14135"
     CDS             complement(3958..5244)
                     /locus_tag="PLP58_14135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009959449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetylhomoserine
                     aminocarboxypropyltransferase/cysteine synthase"
                     /protein_id="HRJ09713.1"
                     /translation="MKLETLCLHGGQQPDPATGARAVPIYRTASYVFKNTEHAANLFA
                     LRELGNIYTRLMNPTTDVLEKRVALLEGAPEMGGLGVASGTSAVFYSIINLAQAGDNI
                     VSARNLYGGTYTQFNDILPALGITVRFVDSNDPENFRKAADDKTRAFFCETVSNPALE
                     IADLEALSKIARENGVPLIVDSTFSTPYLTRPLDHGADIVVHSLTKWFGGHGAGIGGV
                     VVDSGKFNWAAGKHPLYTTPDHSYHGLRWGMDLPAPLLPLAYILRMRTVPLRNLGACI
                     APDNSWFLLQGIETLPLRMERHCENALAVARHLKKHPRVTWVRFPGLEDDPQHAASRK
                     YLRGKGGSMVVFGIKGGREAGARFIDSLKLFSHLANVGDAKSLAIHSASTTHSQLNEE
                     QQAAGGITPDLVRLSIGLEHMDDILADLDQALEAAA"
     gene            complement(5307..6095)
                     /gene="rph"
                     /locus_tag="PLP58_14140"
     CDS             complement(5307..6095)
                     /gene="rph"
                     /locus_tag="PLP58_14140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012419449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004549 - tRNA-specific ribonuclease
                     activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HRJ09714.1"
                     /translation="MPRTDGRLPDQLRPVEIFLDIAPHATASVLIGFGRTRVICAATI
                     QDEVPRWMKVQGVPGGWLTAEYSMLPYSTLDRKERDSSRGRPDGRGIEIQRLIGRSLR
                     AVVDLKKLGQRTLCIDCDVLQADGGTRTASITGACIAAAIAFNRLLEKGKLTQQPLTK
                     KVAAISAGILKGETLLDLCYEEDRDADVDCNLVLTEDGEFVEIQGSGEEATFTQAQMD
                     AMLVHGRKGVMELCALQQKAIEGGMKPAAPADLQNLADFYGKKS"
     gene            complement(6167..6991)
                     /locus_tag="PLP58_14145"
     CDS             complement(6167..6991)
                     /locus_tag="PLP58_14145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ09715.1"
                     /translation="MDLQKFLFLAAAVVFGLACRSFDNRYLLKLGWLSYLGASYLAGF
                     FLTGSHVAGAFGVSLWFLLPWVEIVGYVRFLRFPERSEVKHRFAPSQEVFPELEELTE
                     DMIEEGFEKVEDTGWNWGGADHFVRLFAHEERRAQAAITQVRFEKIDIVFSYVSVTSR
                     ALDGRIFTTTNYPFAPAMRFVPGQIVNREVHLDTLGEMMSSHEDFLDAHGLGQEDLEE
                     ADPENLASVIERDLGAQIEHNLKIGVLEPAGEDGVRYSWRGCFYLWFEAVKDMVRV"
     gene            complement(7083..7991)
                     /locus_tag="PLP58_14150"
     CDS             complement(7083..7991)
                     /locus_tag="PLP58_14150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009966135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="manganese/iron ABC transporter ATP-binding
                     protein"
                     /protein_id="HRJ09716.1"
                     /translation="MHATAPPLSIEVADVTVAYANGHIALRDATFSLGAGTICALVGV
                     NGSGKSTLFKSVMGFLRPAKGRVRIAGMEVAQARKRQLVAYVPQAEEVDWTFPVSVWD
                     VVMMGRYGHMNFLRIPRAEDRRLALECLKRVGMADFAERQIGELSGGQKKRVFLARAL
                     AQQGRVILLDEPFTGVDVQTEAAIIELLRALRDEGHIILVSTHNLGSVPEFCDHVVLI
                     NRTVLASGPTEEVFTEDNLSRAFGGVLRQFHFEHSTIQDHDGRTVKLLTDDERPLVFG
                     KGGHLEYTERQGREGIVERREKQEGA"
     gene            complement(7997..8896)
                     /locus_tag="PLP58_14155"
     CDS             complement(7997..8896)
                     /locus_tag="PLP58_14155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009966136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding protein"
                     /protein_id="HRJ09717.1"
                     /translation="MKHLPPLLATALCLASCGPAPEAPADGKKRVLTTFTILQDMAQN
                     VAGDAAVVESITKPGAEIHDYEPTPMDLVKAQRADLVLWNGLGLERWFEKFLQQVKDA
                     PGVVLSEGVEPMGIGEGPYTGKPNPHAWMSPANAVIYVENIRKALVKLDPANEAAYSA
                     NAAAYTEKIRAVDEPVRKTLAAIPEEQRWLVTCEGAFSYLTRDYGLKELYLWPINADE
                     EGTPQQVRKVVDTVREKKIPAVFTESTISDKAMLQVAKETGARFGGTLHVDSLTEADG
                     PAPTYLQLLQANADTIVKAFTQP"
     gene            complement(8905..9696)
                     /locus_tag="PLP58_14160"
     CDS             complement(8905..9696)
                     /locus_tag="PLP58_14160"
                     /inference="COORDINATES: protein motif:HMM:NF024946.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter"
                     /protein_id="HRJ09718.1"
                     /translation="MLAYLRFTACLSTVLAAMHARASEPARPMSTDRPDTTESPYTVP
                     AGMFQIEASFFDYSRDSADGNRLREWSWSEINFKAGLARNVDLQVILSAWNESVLTRR
                     GGSERLSGVSDVTVRLKTNLLGNDEGRSAFGLMPYVTIPSGSGVSADAWQGGLIAPFA
                     FEVNDRVSLGLMAEADLVYDEDTGGHDLQWLHSATVGIALTEPLGMYLELVGIAGEDT
                     NYQGIFNAGLTFAVSDDLVFDAGVRIGLNRPAPDFGVFTGVSFRF"
     gene            9828..10274
                     /gene="mntR"
                     /locus_tag="PLP58_14165"
     CDS             9828..10274
                     /gene="mntR"
                     /locus_tag="PLP58_14165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009966139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator MntR"
                     /protein_id="HRJ09719.1"
                     /translation="MPAKRQETTAHVHSPAVEDYLEQIHNLIAGKGYARVVDIAKNLG
                     ISQASVTNMIQKLDAEGYVVYERYRGVVMTEAGRKIGQEIARRHEVLTRLLSGFGLDS
                     ATVHEDVEGMEHHMSRQTLDVLTLLMEELEANPDLLKKLRRKLARA"
     gene            10303..11133
                     /locus_tag="PLP58_14170"
     CDS             10303..11133
                     /locus_tag="PLP58_14170"
                     /inference="COORDINATES: protein motif:HMM:NF015338.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HRJ09720.1"
                     /translation="MRRHSLSGFRFIVPVLLALAASLSGSSAQEAGRALRVLCYNIHY
                     GQGNDGEYDLARLAEVIKAAKPDLVGLQEVDVGVRRSGKAHQVLRLAELTGMAARYGP
                     TQHYEGGLFGNAVLTRLHILDVAIHPLPYTEATLEKTTYPRGAIAVTVRAPDGQPLRF
                     ISTHFQHNVEEDRVAEAHAINELFAKPGDTLRTILAGDLNAKPDAEPIRVLEGRWINA
                     LDPAAAPTAPSHKPVSRIDYILSRPEAAFRLVSSQVVPDDMASDHRPVLAVFELAGKK
                     "
     gene            11193..12071
                     /locus_tag="PLP58_14175"
     CDS             11193..12071
                     /locus_tag="PLP58_14175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ09721.1"
                     /translation="MRTNPLFFFAILCQLPALAGAQEVEKYVEINPGTLPVILLAPHG
                     GSLKPEGVAERRFGKFLTDANTADLTRRIADAMLEKYGARPVSILCLMHRSRVDCNRE
                     LEEASQGDPVAAAVWERFHGAAAEACKRVSQMHGAGLVLDIHGHRHDPARVELGYLLG
                     AATLDLDDAALDSGDALSRSSIRALVEKSGRPLSEILRGPRSLGSFLEAQGIAAIPGP
                     ALPSPKGADYFSGSYNIQAHGSRDGGTVSAIQVECPWEGVRDTPEKRTEFARKLATAL
                     GGYFEAHFGAGLGSKK"
     gene            complement(12060..>13656)
                     /locus_tag="PLP58_14180"
     CDS             complement(12060..>13656)
                     /locus_tag="PLP58_14180"
                     /inference="COORDINATES: protein motif:HMM:NF012869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRJ09722.1"
                     /translation="RVFDEVIPSRDLAALGRLCAWLAAAFLLREVVAAFRAWSAGVFE
                     QRMNHELRARLHRKLLQMPLCWHKRRKTGDQLARMMDDAPAAQRAVLEGVEQGLTALL
                     QILVCAGVMFDTSPRYALVILLPVPFIAAGGWIFSRLLAPRAARAREAGARVGSFLHE
                     TLAGIRLIKSHTAETARQHGFEDVSAAAGRGQRRLAAAWALYSPLMCLMGGLGLVLLL
                     GTGARGVLEGEITSGELLKLILLLGFFYEPVARLHGVNQTLVSGLAGAARVFEVLDAE
                     GAEAGRLDSPPHAARGEIRFKNVTFGYDEGMPVIHGISLHVPAGQTCAIVGVSGCGKS
                     TLFDLLAGFYRPWSGVIEMDGVSLAGRDLAALRARTAYVMQDAFLFSGTLRANLLLAR
                     PDAGDAELWAALREARAEDFVKARPDGLDTEVGERGVLLSGGERQRLALARAFLKDAP
                     VLLLDEATSAVDAESERHIQEALERLCESRTCMVIAHRLSTVARADVIHVMSAGRVIA
                     SGSHAELLEGCPHYRHLASLSFL"
BASE COUNT         2517 a         4558 c         4217 g         2364 t
ORIGIN      
        1 gaccggcagg gtgggagcgg caggcagggc gcccatgacg gcggcgggga tgggcggctc
       61 ctcgatctgc gcggccgcat ttgggggcag ggcgccgggc cgttcgatgc gctcctcgat
      121 gacgatgcgg tctttgaaca aatacaatgc cacgaggctg ccgatgctga ccagggtgac
      181 ggtgaggtga agaccgactt tgagcccgtc ccaaaggaag gcgtcgagct tctgcatgaa
      241 ggtgcggccc tggcgctgga tgggctgcgg cggtggcggc acgggacggc gcgcgggcgg
      301 ggtggcggca ggctgagaaa agtctttcgc cggaggagcg tcggcggcct tgggtgcggc
      361 ggcttctttt ttgggctttg cctggcgggc gtcgagcagg gcatgaccag cttgcgcggc
      421 actggtgtat tggcgggcag gatcggtttc cagacagcgc cggagaacga gggcaaaggc
      481 gggcggcagt tcgcgcgcgg tgcggccatc cggggatggc ggctgacggg cgaacatttc
      541 atgcagagtg gccccgagag cccgaatatc ggcggctttg ctgctggtgt cgccatgaat
      601 ccaggtcagc tcgccggtct ccagccttgc catgccgatg ccggtgagct tcaattgtcc
      661 cgccgaggta ataagcacgg tccgcgggct gagcgcgccg tggtgcaggc cgtggtcatg
      721 gaccgcctgc agcgcggagc aaagcggcag cgccagggtg aaggcggtct tgggcgtgat
      781 ctccctgcct tgaatctgct cggcaatggc gcgcgcctcg atgtgctcgc tgattaggta
      841 ccagagtccg gcgggcgatt tgcccacatc gtagaccgcc acgcagttcg gatgcaccag
      901 cttggcgcgc gcccgcagcc gggccagcat gagggactcc tcctgaccgc cgggattccg
      961 catgactcgg atgtccacca gccgctccaa agccgtctgc cgcgccagat aaacagcgct
     1021 gtcccggccc gactccaggc gccgcagcac ttcaaaggct ggcagccagg ccgacagctc
     1081 ctggagagag ggaaaagtgc tggaattggg gacggccatt caaaattctg gactggaatg
     1141 cgaattttag cgcagatcac cggccaggcg agcacgcatc ttgggtctat gcagattgaa
     1201 aatgaggaat ttcagtcgga tgacaatttc catataaaac agatagacaa tatggtatta
     1261 atgcttgcct gtccttgcat tcgaggtaca ctctgcgaga ctgcccgcct ttttccaccc
     1321 tcaagcaccc acccccatga gcaaagcctc cgttgaagac atcaagctca cctccgccgg
     1381 cctccgcggc cagttggccg agctgttcgc cgacaccagc acaccgaaca tcccggagga
     1441 cagcaacatt ttgctcaaac accacggctc ctaccagcag gacgaccgcg accgccgcgc
     1501 cgcactcgtg aaagccaagc aggaaaaagc ctggagcttc atgatccgca gcaagatgcc
     1561 cggaggccgc atttcggcga agcagtggat gatccatgac gaactctgca cgaaagcctt
     1621 cggcaacatg cgcctgacga accgccaggg catccagctc cacggcgtgc tcaaaggcgg
     1681 gctgaaagaa gtcatccaca acgtctgcca cagcggcctg agcaccatgg gcgcctgcgg
     1741 tgacgtggtg cgcaacacga tgggaccggc cgcgccggtc aaagacgccg tccatgccga
     1801 cacgcagcgc ctgaccgagg agatcagcca gcgctttttg tggcgctcca gcgcttacgc
     1861 cgacatctgg ctcgacgggg aaaaagtgga gccggaatgg atcaaggacc ccgcggtaaa
     1921 ccccgccgtg cgcgaggcga cccaggcccc cgaaggcgac gaccccatct acggcaaggt
     1981 ctatctgccg cgcaaattca agatcggcgt ggccatccag ccctgcaatg acgtggatgt
     2041 tttctcccag gacgtgggct ttgtcccgca tgtggtggag ggccgggtgg aaggctacac
     2101 catcaccgtc ggcggcggct tcggcatgag ccacggccag ctcgccacgc gccccttcct
     2161 cgcccagccg cttctgtatg tgaagcgcgc ccatgtggtg gacgcctgcg aggccatcgt
     2221 caccacccag cgcgaccatg gcaaccgcgc cgagcgcaag cacgcgcgtc tcaaatacac
     2281 ggtgcagagc atggggatcg aagccttccg cgccgaggtc gtgcgccgtc tgccaggcat
     2341 cgagaccttc ccgccgaagg agctgaaatt tgacagtgtt gaggacaccc tcggctggca
     2401 tgagcagggg gatggcaagc tttactgcgc cgtctatgtg agcatgggcc gcatcgtgaa
     2461 ctccgaaaac ggcccgcaat accgcggcgc cttcgccgag atcgccaccc ggctcgacct
     2521 gcccttcatc gtcacgccaa acacgaacct catcatcgcc gatgtggcgc cggagcaaaa
     2581 ggagcaggtc aatgccatcc tggcaaagca cggtgtcgtc cacgccgacg aggccggctt
     2641 cacccgtgcc cgcaaggtgg cccacgcctg cgtggccctg cccacctgcg gcctcgcgct
     2701 gagcgaatcc gagcgcgccc ttcccggctt catggatcag tttgacgcca tgctccagga
     2761 actcggcatc gtggatgagc cgatcctttt ccgcatgacc ggctgcccga acggctgcgg
     2821 ccgcccctac aacgccgact tcggcttcgt gggccgcgcg ccgaacaagt gggccatgtt
     2881 cgtcggcggc agcatccgcg gcgacacgct cgccggcctc gaattcaagt ccgtcaccgg
     2941 cgaggagatc cccggcaaag tccgcgccat cctcgaagcc ttcattgcgg agcgccagcc
     3001 gggggaaatc ttcgccgact ggttcgcccg cacccgcacg aagggggcag agccgacttc
     3061 cgagcagttc cacatcgaac tcgccgagcg cgccgcgaag ctcgccgggg agaaggcagg
     3121 cgaggcgggc tgaggcatcc ttgctgaatc atcgcgcgct ggcgcggcgt tgaatctcca
     3181 tgcaactcgc cgagatgacc tggcctgagg tggccgcttt gccaaggcac acccccgtgg
     3241 tcttccccgt cgccgccctg gagcagcacg ggcgccacat gccgctcttc accgactcgc
     3301 ttctcatggg cgagatcgcc cgccgcacgg aggaggagtt gcgcggaagc gcgctcttcg
     3361 cgccgctgca atggctgggg aactcgcatc atcacttgga tttccccggc acgctctccg
     3421 ctggtccgcg cgtctatctc gacctcctct gtggtctgct ggaaaatttc attgcccacg
     3481 gcttcactcg gctgctcatc ctcaatggtc atggtggcaa cgacgtgcca ggccgtcagg
     3541 caatcttcga agtccgacaa aggcaccgtg agcggaagga cttgctcctg ctctttgcca
     3601 cctactggaa tctggcgcct catgctcatg aggcccatcc cggcctttct caacgacaaa
     3661 tgggacacgc ttgcgagtgg gagacctcga tgattttgcg tttgtctccg catctggtca
     3721 aaggccacgc acaagctgtg gaagtacctt ttggctgccc cttcgaaccc gccgcgcgcg
     3781 gctggactat gccggatcgc agcgcgcccg gacatgttgg tgatcctcgg gcggcttcgg
     3841 cggaaaaggg cgaggcactg tttcaggctt tcaatgcggc caccgtcgcc atgctgcggc
     3901 gcatgattgc ctgggacggg aaaagctggg agggttaaac ccgggaacgg cagcagttca
     3961 agccgctgcc tccaacgcct ggtcgaggtc ggcaagaatg tcgtccatgt gctccaagcc
     4021 gatgctcaag cgaaccaagt caggcgtgat gccccctgcg gcctgctgct cctcattgag
     4081 ctggctgtgc gtggtgctgg cgctatggat ggcgagcgac ttggcatccc ccacattggc
     4141 gaggtgggag aagagcttca ggctgtcaat gaagcgcgcc ccggcctcgc gtccgccttt
     4201 gatgccgaag acgaccatcg agccgccctt gccgcgcagg tatttccggc tggccgcgtg
     4261 ctgcgggtca tcctccaggc cgggaaagcg cacccaggtc acgcgcggat gctttttcag
     4321 atgccgtgcc acggccaggg cgttctcgca gtgccgctcc atgcgcagcg gcagagtctc
     4381 gatgccctgg agcaggaacc acgaattgtc cggagcgatg cacgcgccca ggttccgcag
     4441 cggcaccgtc ctcatgcgca ggatgtaggc cagcggcagc agcggcgcgg gcaggtccat
     4501 gccccagcgc aggccgtggt agctgtggtc cggcgtggta tagagcggat gttttcccgc
     4561 tgcccagttg aatttgccgc tgtcaaccac cacgccgccg atgccggcac cgtggcctcc
     4621 gaaccacttc gtcaacgaat gcaccacgat gtccgcgcca tgatccagcg ggcgcgtgag
     4681 gtagggcgtg ctgaaggtgc tgtccacgat gaggggcacg ccgttttcgc gggcgatctt
     4741 tgagagtgcc tccaaatcgg cgatttccag cgccgggttc gacaccgtct cgcagaaaaa
     4801 agcccgtgtc ttgtcatccg ccgccttgcg gaaattctcc gggtcattgc tgtccacaaa
     4861 tctcaccgtg atgcccagcg cgggcaggat gtcattgaac tgcgtgtaag tgcctccgta
     4921 aaggttgcgg gcggagacaa tgttgtcacc cgcctgcgcc aggttgatga tggagtaaaa
     4981 caccgcgctg gtgccgctgg caacgcccag tccacccatc tccggcgcgc cttccagcaa
     5041 agccacgcgc ttctccagca cgtccgtcgt cggattcatc agccgggtat aaatgttccc
     5101 cagttcgcgc agcgcgaaaa ggttcgccgc atgctccgtg tttttgaaga catagctggc
     5161 ggtgcggtag atcggcacgg cgcgcgcgcc ggtggctggg tcgggctgct ggccgccgtg
     5221 aaggcagagg gtttcaagtt tcatggccgg gttgggatgg tggaaaaaat cgcgcctgct
     5281 attttgtgcg gcttttgccg gcccgctcag cttttcttgc cgtaaaagtc cgccaggttt
     5341 tgcagatcgg cgggtgcggc gggtttcatc ccgccctcta tggccttctg ctgcagggcg
     5401 cacaattcca tcacgccctt gcgcccatgt acaagcatgg catccatctg tgcctgggta
     5461 aaggtggcct cctcgccgct gccctggatt tcgacaaact cgccgtcctc ggtcagaacg
     5521 aggttgcagt ccacatcggc gtcgcggtcc tcctcgtaac agaggtcgag cagcgtctca
     5581 cccttgagga tgccggcgct gatggcggcg acttttttgg tcagcggctg ctgggtgagc
     5641 ttgccctttt ccagcaggcg gttgaaggcg atggcggccg cgatgcacgc gccggtgatc
     5701 gaagcggtgc gcgtcccgcc gtcggcctgg agcacatcac agtcaatgca cagagtccgc
     5761 tggccgagtt tcttgaggtc caccacggcg cgcaggctgc gtccgatgag gcgctgaatt
     5821 tcaatgccgc gaccgtccgg cctgccgcgc gagctgtccc gttctttgcg atcgagggtc
     5881 gagtacggca gcatcgagta ctcggcggtg agccagcctc cgggcacgcc ctgcactttc
     5941 atccagcgcg gcacctcgtc ctggatcgtg gccgcgcaga tgacgcgcgt gcggccgaag
     6001 ccgatgagca cggaggcggt ggcgtgcggg gcgatgtcga gaaaaatctc gaccgggcgg
     6061 agctggtcgg ggaggcggcc gtcggttctg ggcatggcgg cccttagcac aaggccgcgc
     6121 gggctgccaa gcgggaatgc ggtcgcgctt caaacagcgt cctggatcag actcgcacca
     6181 tgtccttcac cgcctcgaac cacaggtaaa aacagccgcg ccacgagtag cgcacgccgt
     6241 cctcgccagc cggctccagc acgccgattt ttagattgtg ctcgatttgc gcgccaagat
     6301 cacgctcgat gacagacgcc agattttccg ggtcagcctc ctccaggtcc tcctgaccca
     6361 ggccatgcgc gtcgagaaag tcctcgtggc tgctcatcat ctcacccagc gtgtccaaat
     6421 gcacttcccg gttcacaatc tggccgggga cgaaacgcat cgccggggca aaagggtagt
     6481 tcgtggtggt gaaaatgcgc ccgtccaacg cgcgcgaggt gacgctgaca taggagaaaa
     6541 caatgtctat cttctcaaac cgcacttgcg tgatggcggc ctgcgcgcgc cgttcctcat
     6601 gcgcaaaaag ccgcacaaag tgatccgcgc cgccccaatt ccagcccgtg tcctccacct
     6661 tctcaaaccc ctcctcgatc atgtcctccg tcagttcctc caactccgga aagacctcct
     6721 gcgagggggc gaagcggtgc ttcacctcgc tgcgctccgg gaaccgcaga aaccgtacat
     6781 aacccacgat ctccacccac ggcagcagga accacaagga caccccgaac gccccggcca
     6841 catggctgcc ggtcaaaaag aagcctgcca gataagaagc acccagatag gacagccagc
     6901 ccagcttcag gagatagcgg ttgtcaaaac tgcggcaagc aaggccgaaa acgacggcgg
     6961 cggccagaaa aagaaatttt tgcagatcca ttgttagatt cggcgtgccg gagccggccc
     7021 gattggtttt tcatggtagc cccttcccgc aaggcggcaa ccctttctcc gcctcccggc
     7081 tgtcaggcgc cctcctgttt ctcacggcgc tccacaatcc cctcgcggcc ctgacgctcg
     7141 gtgtattcga gatggccgcc tttgccgaag acgagcggac gctcatcatc cgtgagcagc
     7201 ttgaccgtgc ggccgtcgtg gtcctggatg gtgctgtgct cgaagtggaa ctgccgcagc
     7261 acgccgccga aggcgcggct gagattgtcc tcggtgaaga cctcctcggt ggggccggag
     7321 gctagcacgg tgcggttgat gagcaccaca tgatcacaaa actcgggcac gctgcccagg
     7381 ttgtgcgtgg agaccagaat gatgtggcct tcatcccgca gcgcacgcag cagctcgatg
     7441 atggccgcct ccgtctgcac atccacaccg gtgaaaggct cgtcgagcaa gatcacgcgc
     7501 ccctgctggg ccagcgcgcg cgccaggaag acgcgctttt tttgcccgcc ggaaagctcg
     7561 ccgatctgcc gctccgcgaa gtccgccatg cccacgcgct tcaggcattc cagcgccagg
     7621 cgccggtcct ccgcgcgcgg gatgcgcagg aagttcatgt ggccgtagcg gcccatcatc
     7681 accacatccc acacactcac ggggaaggtc cagtccacct cctccgcctg cggcacatag
     7741 gccacgagct ggcgtttgcg cgcctgcgcc acctccatgc ccgcgatgcg cacccgccct
     7801 ttggcgggcc gcagaaagcc catgaccgac ttgaatagcg tgctcttgcc gctgccgttc
     7861 acgcccacca gcgcgcagat ggtgcccgcg ccgaggctga aggtggcgtc ccgcagcgcg
     7921 atgtgcccat tcgcataagc cacagtcacg tcggcgacct caatggagag cggcggggcg
     7981 gtggcgtgca tggatttcaa ggctgcgtga acgccttcac gatggtgtcg gcattggctt
     8041 gcaacaactg caaatacgtc ggtgcaggtc cgtcagcttc ggtcagcgag tccacatgaa
     8101 gcgtgccgcc aaagcgcgcg ccggtctcct tggccacctg gagcatggct ttgtcgctga
     8161 tcgtgctttc cgtgaagacg gcggggatct tcttctcccg caccgtgtcc accaccttgc
     8221 gcacctgctg cggcgtgcct tcctcgtcgg cattgatggg ccacaaatag agttccttca
     8281 aaccgtagtc gcgcgtgagg taggagaacg cgccctcgca ggtgacgagc cagcgctgct
     8341 cctcaggaat agcggcgagg gtcttccgca ccggttcgtc cacagcgcgg attttctccg
     8401 tgtaagcggc ggcgttggcg ctgtaggcgg cctcgtttgc gggatcgagc ttcaccagcg
     8461 ccttgcggat gttttcgaca tagatgacag cgttggcggg cgacatccag gcgtgcgggt
     8521 ttggtttgcc cgtgtaggga ccctcaccga tgcccatcgg ctccaccccc tcgctgagca
     8581 ccacgccggg cgcgtccttc acctgctgga ggaatttttc aaaccagcgc tccaggccga
     8641 gcccgttcca cagcaccagg tcggcgcgct gcgctttcac gaggtccatc ggtgtcggct
     8701 catagtcgtg gatctccgcg ccgggcttgg tgatggactc gacgacggcg gcgtcaccgg
     8761 ccacgttttg cgccatgtcc tgcaggatgg tgaaggttgt taggacgcgc tttttgccat
     8821 cggcaggcgc ttcaggcgcg gggccgcagg aggcgagaca aagcgcggtg gcgagcaggg
     8881 gggggaggtg tttcatgttg ggaattaaaa gcgaaagctg actcctgtga agacgccgaa
     8941 gtccggagcg ggccggttga gtccgatgcg gacacccgcg tcgaagacga gatcatcact
     9001 cactgcaaat gtgaggcctg cattgaaaat gccctggtag tttgtgtcct ctcccgcgat
     9061 gccgaccagc tccagataca tgcccagcgg ctccgtcagc gcgattccca cagtagccga
     9121 gtgcagccac tgcaggtcat gaccgcccgt gtcctcgtca tagaccaggt ccgcctcggc
     9181 catgagaccg agcgagacgc ggtcattcac ctcaaaggca aagggagcga tcaaaccgcc
     9241 ctgccaggcg tccgcgctca caccactgcc gcttggaatc gtcacatagg gcatgagacc
     9301 aaaggcggac ctcccctcgt cattgcccag gaggttggtc ttcaagcgga cggtcacatc
     9361 gctgactccg gagagccgct cactgccgcc gcggcgggtc aaaacggatt cattccaggc
     9421 gctgaggatg acttgcagat ccacattccg cgccagtccg gctttgaaat tgatctcgct
     9481 ccatgaccac tcccgcagac ggtttccgtc cgccgaatcg cgcgaataat cgaaaaagct
     9541 ggcctcgatc tggaacatgc cggcgggcac cgtgtaaggg gactcggtcg tgtccggacg
     9601 gtccgtgctc atggggcgcg caggctccga cgcgcgagcg tgcatggcgg cgaggactgt
     9661 ggaaaggcag gcggtgaaac ggagataggc gagcatgata aagttagacc cagctaaaaa
     9721 gatcagcgcc gccaatctgg caagcgaaaa gttagcctgg cctaaaacta ttttctccaa
     9781 agcaaaattt cgtttcccgc accggcccct gctgctatcg gtccccgatg cccgccaagc
     9841 gccaggaaac cacagcccat gtccactcgc ccgccgtcga ggactacctc gagcagattc
     9901 acaatctgat cgcggggaaa ggttacgccc gcgtggtgga catcgccaaa aacctgggca
     9961 tctcccaggc cagcgtcacc aacatgatcc agaagctcga tgccgagggt tatgtggtct
    10021 atgagcgcta ccggggcgtg gtgatgaccg aggcgggaag gaaaatcggc caggaaatcg
    10081 cccgcaggca cgaggtgctc acgcggctgc tttccggctt cgggctggac tccgccacgg
    10141 tgcatgagga cgtggagggc atggagcacc acatgagccg ccagactctg gatgtgctca
    10201 cgctcctgat ggaggaattg gaggccaatc ctgatctttt gaaaaagctc aggcgcaagc
    10261 tggcgcgcgc atgagcaggc ctctgatgcc cgtatcgctg gcatgagaag acacagcctt
    10321 tccggtttcc gcttcatcgt ccccgtcctc ctggcgctcg ccgcgtcgct ttccggctcg
    10381 tccgcgcagg aggcgggccg cgctctgcgc gtgctctgct acaacattca ctacggccag
    10441 ggcaatgacg gcgaatacga tctggcacgt ctggcggagg tgatcaaagc cgccaaaccc
    10501 gatctcgtcg gactccagga agtggacgtt ggcgtgcgcc gctccggcaa ggcgcaccag
    10561 gttctgcggc tcgcggagct gaccggcatg gccgcgcgct acggccccac acagcactat
    10621 gagggcggcc ttttcggcaa cgccgtgctc acccgactgc acatcctcga cgtggccatc
    10681 catcccctgc cctacaccga ggccacgctg gaaaagacca cctatccgcg cggtgccatc
    10741 gccgtcaccg tgcgcgcgcc ggacgggcag ccgctgcgtt tcatcagcac gcatttccag
    10801 cacaatgtcg aggaggaccg cgtggccgag gcccatgcca tcaacgaact cttcgccaaa
    10861 cccggcgaca ctctgcgcac catcctggca ggcgacctca atgccaaacc tgacgccgag
    10921 cccattcgcg tcctcgaagg acgctggatc aacgcccttg atcccgccgc cgccccgacc
    10981 gcgccctcgc acaagccggt ttcgcgcatt gactacatcc tctccaggcc ggaggcggcc
    11041 ttccggctgg tgagttccca ggtcgtcccc gatgacatgg catccgacca ccggcccgtg
    11101 ctggcggtgt ttgaattggc cgggaaaaag taaacgcctc cccaaaatgg gtggctcgac
    11161 attccggcgc gcatgttcca gccttggcgt gcatgagaac caaccctctt ttctttttcg
    11221 ccattctgtg ccagctgcca gccctcgcgg gcgcgcagga ggtggaaaag tatgtcgaaa
    11281 tcaaccccgg caccctgccc gtcatcctgc tggctccgca tggcggctcg cttaaacctg
    11341 aaggcgtggc cgagcgccgg tttggcaaat ttttaaccga cgccaacacc gccgacctga
    11401 cccgccggat cgccgatgcc atgctggaga aatacggcgc gcgccccgtc agcattctct
    11461 gcctcatgca tcgctcgcgc gttgactgca accgcgagct ggaggaggcc tcgcagggcg
    11521 accccgtggc cgccgccgtg tgggagcggt ttcacggcgc agccgccgaa gcgtgcaagc
    11581 gcgtcagtca aatgcacggc gcgggtctcg tgctggacat ccacggacac cggcacgacc
    11641 cggcccgcgt ggaactcggc tacctgctcg gagccgccac gctggacctc gacgacgccg
    11701 cgctcgactc cggtgacgcg ctctccaggt ccagcatccg ggcccttgtg gaaaagtccg
    11761 gcagaccgct ctcggaaatc ctgcgcggcc cgcgaagcct cggctctttt cttgaggcgc
    11821 aaggcatcgc cgccatcccc ggcccggccc tgccctcccc caagggcgct gactacttca
    11881 gcggcagcta caacatccag gcccacggct cgcgtgatgg cggcacggtc agcgccattc
    11941 aagtcgaatg cccttgggag ggagtgcgcg acacgccgga gaagcgcacg gaattcgcgc
    12001 gcaagctcgc caccgccctc ggcggctatt tcgaggcgca ctttggcgcc ggtctcggct
    12061 caaagaaatg acagcgaggc cagatggcgg taatgcgggc agccctccag cagctccgcg
    12121 tggctgccgc tggcgatgac acgccccgcg ctcatcacat ggatcacatc cgcgcgcgcc
    12181 accgtggaca gtcggtgcgc gatgaccatg caggtgcggc tttcacacag ccgctccaga
    12241 gcctcctgga tgtgcctctc cgactccgca tccacggcgg aagtcgcctc gtccagcagt
    12301 agcacgggcg cgtctttcag aaacgcccgc gccagcgcca gccgctgcct ttcaccgccg
    12361 ctgagcagga ctccgcgctc gcccacctcc gtgtcgaggc catccggcct ggctttgaca
    12421 aaatcctccg cgcgcgcctc gcgcagagcc gcccacaatt ccgcgtcacc ggcatcaggc
    12481 cgcgccagca gcaggttcgc ccgcagcgtg ccggagaaaa gaaaagcgtc ctgcatcaca
    12541 tacgccgtgc gcgcgcgcag agcggccagg tcccgcccgg cgagagacac tccatccatc
    12601 tcgatgacac ccgaccacgg gcggtaaaaa cctgccagca aatcaaagag cgtggacttt
    12661 ccgcagccgc tgacaccgac aatggcgcat gtctgcccgg cgggcacatg caggctgatc
    12721 ccgtggatca caggcattcc ctcgtcataa ccaaaagtca cgtttttgaa acggatctcg
    12781 ccgcgcgcgg catgcggcgg ggagtcgagc ctgcccgcct ccgcgccctc cgcgtccagt
    12841 acttcaaaca cacgcgccgc tcccgcaagg ccgctgacca gggtttggtt cacgccgtgc
    12901 aaacgcgcca ccggctcgta aaaaaagccg agcagcagga tgagcttgag cagctcgccg
    12961 ctggtgattt ccccttccag tacgccgcgc gcccctgtgc ccagcagcag caccagcccc
    13021 aggccgccca tcaggcacat cagcggactg tacaacgccc atgccgccgc cagccgccgt
    13081 tgtccgcgcc ccgcagccgc gctcacatcc tcaaacccgt gctgacgcgc cgtctccgcc
    13141 gtgtggcttt tgatcagccg gatgccggcc agcgtctcgt gcaggaatga gcccacccgc
    13201 gcgcccgcct ccctggcccg cgccgcgcgc ggagccagca gccgggagaa aatccagccg
    13261 cccgcggcga tgaagggcac aggcagcagg atgacgagcg cataacgcgg actcgtgtca
    13321 aacatgaccc cggcgcagac aaggatttgg agcagcgcgg tcagcccctg ctccacgccc
    13381 tccagcaccg cgcgctgcgc cgccggcgcg tcatccatca tgcgcgcgag ttgatcgccg
    13441 gttttgcgcc gcttatgcca gcagagcggc atctggagga gcttccggtg cagccgcgcg
    13501 cgcagctcat gattcatgcg ctgctcaaag acacccgccg accaggcgcg aaaagcggcc
    13561 accacctcgc gcagcaaaaa cgcggcagcc agccaggcgc agagccgccc cagcgcggcc
    13621 aggtcccggc ttgggatcac ctcgtcaaag acccgc
//
LOCUS       DAOMYJ010000138        13463 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Prosthecobacter sp. isolate AMR_MDS_5629
            NODE_1532_length_13463_cov_20.939290, whole genome shotgun
            sequence.
ACCESSION   DAOMYJ010000138 DAOMYJ010000000
VERSION     DAOMYJ010000138.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867850
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prosthecobacter sp. (wastewater metagenome)
  ORGANISM  Prosthecobacter sp.
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Verrucomicrobiaceae; Prosthecobacter.
REFERENCE   1  (bases 1 to 13463)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13463)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.30
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:34:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,742
            CDSs (total)                      :: 4,684
            Genes (coding)                    :: 4,676
            CDSs (with protein)               :: 4,676
            Genes (RNA)                       :: 58
            tRNAs                             :: 55
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13463
                     /organism="Prosthecobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1532_length_13463_cov_20.939290"
                     /isolate="AMR_MDS_5629"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1965333"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(10..273)
                     /locus_tag="PLP58_14185"
     CDS             complement(10..273)
                     /locus_tag="PLP58_14185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ09723.1"
                     /translation="MSEENTPTPPPPSSPKTSAVPLKKETVRITLRARPEGGATVPRE
                     ATAPVSPVEGGLPPADTPKRSTGPVQLPSAPLPPPAGRWPGRQ"
     gene            complement(374..1228)
                     /locus_tag="PLP58_14190"
     CDS             complement(374..1228)
                     /locus_tag="PLP58_14190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009965151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L family deacetylase"
                     /protein_id="HRJ09724.1"
                     /translation="MTVTFRHPKHDVFIPDQTDPWTALRRVTHLGIVSHQDDLEIAAY
                     HGITECFHSEDQWFGGVVVTDGGGSPRKGDYASHTDEEMRQVRLLEQRKAAYVGEYGF
                     MAQLGYPSDEIKKQSDGPVVEDLKFILEHAQPGTLYLHNPCDRHDTHVATFLRCLEAL
                     RALPRELRPQKVYGCEVWRKLDWLLTGDKVMLRVDKHPHLLRPLLGVFDSQISGGKRY
                     DLAEEGLRHANATYFDSHTTDKTSLLTFAMDLTPLVHDDSLDVDDFTLGFVRRLEQDV
                     AQRLKKFR"
     gene            1325..2032
                     /locus_tag="PLP58_14195"
     CDS             1325..2032
                     /locus_tag="PLP58_14195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012420868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HRJ09725.1"
                     /translation="MSTASVAGLLPAHLMPDPEWTDPSSSEPASSGEAELSAFANDEE
                     NVRLMLRVKEGDARAFEKLVELHQGTVIGTACRMLGNLDDAHDIAQQVFIRVWKSAPR
                     YEPTAKFTTWLFTILRNLVFNETRRRGRRHEVPLENENDDDPPRQHADHTAPAPDQIT
                     QQVELEDALDRAIAALPEKQRMAVILRRHEEMPYEQICEILRMSLPALKSLLFRARNE
                     LRKHLASHLDDSPGSDA"
     gene            complement(2073..2147)
                     /locus_tag="PLP58_14200"
     tRNA            complement(2073..2147)
                     /locus_tag="PLP58_14200"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2112..2114),aa:Glu,seq:ttc)
     gene            complement(2223..3551)
                     /locus_tag="PLP58_14205"
     CDS             complement(2223..3551)
                     /locus_tag="PLP58_14205"
                     /inference="COORDINATES: protein motif:HMM:NF016311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase N-terminal domain-containing
                     protein"
                     /protein_id="HRJ09726.1"
                     /translation="MRLLFSLLIYNLLLPAALLAMLPGALAKMRRRGGHWQDLAQRLG
                     FLPPDKRAALDALPPRRVWVHAVSVGEAGIAMKLIPALLARAPDLGVLLTTTTPTGHA
                     LAAEFATRHAGRVVVLYSPVDLPLIGAWPLRLARPEQIILVEAELWPNLAAAACLAGV
                     RMSLVNARLSARSERRFRRFGFFIRPVFRLLDLVCVQEPGDRERFAGLGVREGRAHLT
                     GSIKFDPQGAEADAAQVESLRAVMERCGIRGSRPVLLLASTHPGEEKMLAGMWLRLRE
                     KFGGLSLIIVPRHVERAAEIVAELRLLGISARRRTGADTRMDAAETPAPVLLVDTTGE
                     LRAWQCLATVVVVGKSFLAVGGQNPAEAVMAGKPVLFGPRMENFEPLVELLLRHEGAL
                     QVPDELELERQLGALLAAPEKCRRLGAAGFAALQAHAGATSKTVSLLLRE"
     gene            3711..5153
                     /locus_tag="PLP58_14210"
     CDS             3711..5153
                     /locus_tag="PLP58_14210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018970804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HRJ09727.1"
                     /translation="MRPLLLCLIAMHCALGSTGRPPNFVMIFIDDMGWGDLSCFGNED
                     AATPNLDRLAREGVRFEHFYVNSPICSPSRCALTTGQYPQRWSITSFLNNRADNDRRG
                     VAQWLDPRAATLPGILRQNGYATGHFGKWHLGGQRDVDDAPPITAYGFDESLTNFEGM
                     GAKLLPMTLKPGDTAPGRIWQDAERLGGPVVWMRRSEITAGFVNAAIPFINRAAAAGK
                     PFYINLWPDDVHGPWWPPTSQWGDGSRRRLYLSVLEEMDRQLGPLLEHIRATPALRDN
                     TLVLVCSDNGHETGAGSGGPFRGHKATLHEGGIRSPLIAWGPGLIAESRAGTTNTTSV
                     FAAFDIAPSLLAIAGIAANAGSGVAPSSFDGEDISPLLLGRSTVSRRAPLFWRRPPDR
                     KHAYGSGPLPDLAVRKDEWKLLCDFDGSNSGLYHLMDDPGEISNLAARHPDIADRLTR
                     AVLDWNASMPKDNGQALGKEAPPAPKKKKK"
     gene            5293..6300
                     /locus_tag="PLP58_14215"
     CDS             5293..6300
                     /locus_tag="PLP58_14215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018970017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HRJ09728.1"
                     /translation="MESPYAPPPADEPAAEPRLPAATAVFQRIRDSIARVFVGQDEVI
                     HQVLAALLAGGHVLLEGKPGLGKTHLVLALSRTFGAAFRRIQFTPDLMPSDVTGHTLY
                     DLGSQSFRVRKGPVFTQLLLADEINRAPAKTQSALLEVMQETQVTIDGETHALTPPFM
                     TFATQNPIEQEGTYPLPEAQLDRFLLKVLIDYPDAAEEAAIVKAVTAEAGGRGLSPAH
                     LQPVCSTGDILAAQREAAAVEAVDSVVNYAVELVRATRKHGAIEIGAGTRGAISLIRV
                     AKACALLDGRAYITPGDVKRAALPVLRHRVSLTPEVTISGQDVDQVLETVIRGVEAPR
                     A"
     gene            complement(6319..7356)
                     /locus_tag="PLP58_14220"
     CDS             complement(6319..7356)
                     /locus_tag="PLP58_14220"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009958807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclopropane-fatty-acyl-phospholipid synthase
                     family protein"
                     /protein_id="HRJ09729.1"
                     /translation="MLNLNDLLAKDLLPDAAIRLGIRQRLAATLRRHESLDPEARQAA
                     LMSHIAGLKAGPVAVETDAANEQHYEVPTRFYQLCLGPHLKYSSGYWPEHGTTFDDSE
                     ALMLRMTCERAALQDGQRVLELGCGWGSLSLWMAEHYPNSRITSVSNSRTQKAHIDAE
                     AARRGLANLEVVTANMIHYQGAGEGVFDRCVSVEMFEHMKNYQELMGRIASWLKPGGR
                     LFVHIFTHREFAYHYEAANESDWMARHFFTGGQMPSDDLLLYFQDDLKIEDHWIVNGT
                     HYSRTSEAWLARMDANKAEIMTLFRETYGADQAVKWRAYWRIFFMACAELWGWKDGAE
                     WFVSHYRFVKP"
     gene            complement(7377..7715)
                     /locus_tag="PLP58_14225"
     CDS             complement(7377..7715)
                     /locus_tag="PLP58_14225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009958809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1475 family protein"
                     /protein_id="HRJ09730.1"
                     /translation="MILALRVFFVTVLVSMLAVTAWASGQCALWAIPRGVAAHPWFIA
                     TLFDAYWGFLAFYCWVFYKETALTSRLGWLLGILLLGNIAISIYALIQLLRVPLDARL
                     EDVLLRRRAA"
     gene            complement(7743..9035)
                     /locus_tag="PLP58_14230"
     CDS             complement(7743..9035)
                     /locus_tag="PLP58_14230"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009958811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclopropane-fatty-acyl-phospholipid synthase
                     family protein"
                     /protein_id="HRJ09731.1"
                     /translation="MNSTLTLDSAQAEAIPATASSAEGGFYERLFFFAMRSMRHGCLR
                     IVYPGGRVRLLGDGVSAPQAEMRLRDPRAFFRRCILFGNIGFGESHTAGEWDTDDIAA
                     VITWFIRSITRSQGARASSSRLPFTNLLRLGSRLWHFGRRNHESNSRRNISEHYDLGN
                     DFYALWLDPTMTYSAARFEHAEQSLEDAQRAKYEALCRKLQLKPEDHVLEIGCGWGGF
                     CTHAAREHGCRVTAVTISKEQFDYATARVLREGLADRVTIQLQDYRHVEGRFDKICSI
                     EMLEAVGGEFLDTWFAKCHELLKPEGLLAFQAITTPDCRHAALRKGVDWIQKHIFPGS
                     LLLSVNQLNRAANRTSDLFMHALEDLGSGYARTLKLWHDAFHARLDEVRVMGFSEAFI
                     RKWSYYLKYCEAAFATRNISVIQAVYTRPNNESLHRAW"
     gene            complement(9032..9853)
                     /locus_tag="PLP58_14235"
     CDS             complement(9032..9853)
                     /locus_tag="PLP58_14235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009958814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1365 domain-containing protein"
                     /protein_id="HRJ09732.1"
                     /translation="MPAPDSSTVPFSALYECEVTHRRLRPKEHHFAYRVFYLWLDLDE
                     LDAFSESLALFKRNRWSLFSFFDSDHIPKGPASARENILAVMREAGVDTEEVARVRLL
                     TFPRVLGHIFNPVCFYYAYDAEENPVCAVAEVTNTFHEQKPYVLTGMDWKDRFRLVTP
                     KHFYVSPFSGLDLEFDFKLRLPGEALEIHVDERDDAGLVLLSVLTGKRRELTDASLLA
                     CAVKYPLLTLRVVFLIHWHALRLWMKKLAVHRKAADPHLQTGVYRPHRSIAPTQT"
     gene            10002..>13463
                     /locus_tag="PLP58_14240"
     CDS             10002..>13463
                     /locus_tag="PLP58_14240"
                     /inference="COORDINATES: protein motif:HMM:NF012361.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HRJ09733.1"
                     /translation="MSSEQRKAFPFSEFEPKWQGEWEASKAYRTPNPGDADFDASKPK
                     YFVLDMFPYPSGNGLHVGHPEGYTATDIIGRFKKMNGFNVLHPMGWDAFGLPAEQYAI
                     KTGTHPRVTTEANVANFTKQLKSLGFAYDWDREVNTTDPGYFKWTQWIFLKLYNSYVG
                     DDGKAHPISELEAQGLSREEIDHRRLAYVSEAPVNWCPQLGTVLANEEVVDGKSEVGG
                     FPVERRPMRQWMLRITSYAQRLIDELDGLDWPESIKLLQRNWIGRSEGAQVQFRIADC
                     GLPNDETITVFTTRPDTLFGATYMVLAPEHSLVDAIAAAEWPANTPDTWKGDPSAIRN
                     PQSAIAWYREKTARKSDLERTELAKEKSGVFTGAYAINPVNNERIPIWIADYVLMGYG
                     TGAIMAVPAHDERDFEFAKKFELPIKQVVAAAGKAWGTGVSPVQSQSTGGMPVPHAVP
                     HAVPHALPSSKYLNDRTEIEKKRRNLPHWQQDGKQYFLTWRLADSLPVEKLRELHDLR
                     QQASLLPEDQRQEHDARIFETTSAWLDEGHGSCILKRPELRQIVTTAFSHFDGKRYDL
                     DAYVVMPNHAHVLVTLKPGEDLSKILHSWKSYTAKEINKLLGEEGAVWQDENFDRLIR
                     SREHEWFVRDYIWKNPSEAGLREGEFDWFERTGETPVPHAETLPESAFCENGIAVNSG
                     FLDGLPTPEAKKKITVWLEQNGFGKGTTNFKLRDWLFSRQRYWGEPFPIVWENGQHRS
                     LPESELPLLPPTLEDFKPTGTPEPPLSKATEWVQYSATAKRELNTMPQWAGSCWYYLR
                     YCDARNSERFVGEAAEKYWMGGGKPGGVDLYVGGTEHAVLHLLYARFWHKVLFDLGYV
                     STPEPFQRLVNQGLILGELEYTLYRKPDGTPLSYVANYMPQLAESLRWETLDPEEVER
                     CPQGLGYVWKQDKSIYVEAKSHKMSKSRGNVINPDDIIRDYGADALRLYEMFMGPLEQ
                     VKPWSTAGVVGQSRFLARIWRLVMEVDDEGVWSLSKALQDVPANKQLTKALHETIKKC
                     GEDIEKLSFNTAISAMMVCTTAFTGAEVKPAAAIVTFLKVLSPFAPHLAEELNAQIRA
                     KFPALAAKRFLSDETWPAYDPAALIEDEVEVVFQVNGKLRDKARVPIQATKEEIEKIA
                     LASARVQE"
BASE COUNT         2548 a         4335 c         4200 g         2380 t
ORIGIN      
        1 gctacttgct cactgcctgc ctggccagcg gccagcgggc gggggaaggg gcgcggaggg
       61 aagctggaca ggaccggtgg agcgcttcgg cgtgtccgcg ggaggcaggc cgccttccac
      121 tggagacaca ggggcggtgg cctcgcgcgg cacagtggct ccgccttcgg ggcgcgcgcg
      181 cagggtgatg cgcacggttt cttttttcag gggcacagcc gaggttttcg gcgagctggg
      241 cggcggcggg gtgggcgtgt tttcttcgct catgtgggtg tcaggggaga atgagtgtga
      301 atatttcggc gattttgttt atttagcggg gcggcgcgtc ccgcgtcaat gccatttctc
      361 gccggcggga catttagcgg aattttttca gcctctgcgc cacatcctgc tccagcctgc
      421 gcacgaagcc gagggtgaag tcgtccacgt cgagcgagtc gtcatgcacc agaggagtca
      481 agtccatggc gaaggtgagc aggctggttt tgtccgtggt gtgggagtcg aagtaggtgg
      541 cgttggcgtg gcgcaggccc tcctcggcga ggtcgtagcg tttgccgccg ctgatctggc
      601 tgtcgaagac tcccagcagc gggcgcagca ggtgcgggtg cttgtccacg cgcagcatga
      661 ccttgtcccc ggtgagcagc cagtccagtt tgcgccagac ttcgcagcca tagacttttt
      721 gcgggcgcag ttcccgcggc agcgcgcgca gcgcctccag gcagcgcagg aaagtggcca
      781 catgggtgtc atggcggtcg cagggattgt gcaggtaaag cgtgcccggc tgcgcgtgct
      841 cgaggatgaa tttcaagtcc tccaccacag ggccgtcgct ttgcttcttg atctcgtcac
      901 tcggataacc gagctgggcc atgaagccgt attcgcccac ataggcggcc ttgcgctgct
      961 cgagcagccg cacctgccgc atctcctcgt cggtgtggct ggcatagtcg cccttgcgcg
     1021 ggctgccgcc gccgtcggtg acgaccacgc cgccgaacca ttggtcctcg ctgtgaaagc
     1081 actcggtgat gccgtggtag gcggcgatct ccaggtcgtc ctgatgcgag acgatgccca
     1141 gatgggtcac gcgccgcagc gcggtccacg ggtcggtctg gtcggggatg aaaacgtcgt
     1201 gcttgggatg gcggaaggtc acagtcatcc atcaagcaca gtctgcgcgg gcttgcaacg
     1261 cggcagaaaa aagaccgccg cgctcttgcg caactccgcc gcgcgggcgt ggttgatagc
     1321 cggcatgagc accgctagcg tggcggggct gctgcccgcc catctcatgc cggacccgga
     1381 atggaccgat ccatcctcaa gcgagcccgc ctcctccggg gaggcggagt tgagcgcgtt
     1441 cgccaacgac gaggaaaacg tccggctcat gctgcgcgtc aaggagggtg acgcgcgcgc
     1501 tttcgaaaag ctcgtcgagt tgcaccaggg aaccgtcatc ggcaccgcct gccggatgct
     1561 cggcaacctt gacgacgcgc atgacatcgc ccagcaggtc ttcatccggg tctggaaaag
     1621 cgcgccgcgc tacgagccca ccgccaagtt caccacctgg cttttcacca tcctgcgcaa
     1681 cctcgtcttc aatgaaaccc gcaggcgcgg tcgccgccat gaggtgcccc tggagaatga
     1741 gaacgacgac gatccgccgc gccagcacgc cgaccacacc gcgcccgctc ctgaccagat
     1801 cacccagcag gtggaattgg aggacgcgct ggaccgcgcc atcgccgcgc tgccggagaa
     1861 gcagcgcatg gccgtcattc tgcgcaggca tgaggagatg ccatacgagc agatatgcga
     1921 gattttgagg atgtccctgc cggccttgaa gagcctgctg ttccgcgcgc gcaacgagct
     1981 gcgcaagcat ctggcctccc atcttgacga ctcgccaggc tcggacgcat gaaaacgaaa
     2041 agcccgcctg gtatggcggg cttgatcatt gctggcgacc cctacgagaa tcgaactcgt
     2101 gtcgcatccg tgaaagggat gtgtcctaac cgctagacga aggggtcatg gctcggttga
     2161 gcgggcggtg attgtgccga catcgggcgt gcgcgcaact gaaaaattgg atttctccaa
     2221 agtcattcgc gcagcaaaag cgacactgtt ttgcttgtcg cgccggcatg ggcctgaagc
     2281 gcggcgaagc ctgcggcacc cagtctgcgg catttctccg gcgcggcgag tagggcgccc
     2341 aattgcctct ccaattcgag ctcatcgggc acttgcaacg cgccctcatg gcgcaggagc
     2401 agttccacca gcggctcgaa gttttccatg cgcgggccga acagcacagg cttgccagcc
     2461 atcaccgcct ccgccggatt ctgccctcca acggccagaa aactcttgcc caccacgacc
     2521 acggtggcca ggcactgcca ggcgcgcagc tcgccggtgg tgtccaccag caggaccggc
     2581 gccggcgttt ccgcggcatc catgcgcgtg tcagcgccgg tgcgcctgcg cgcgctgatg
     2641 ccgagcagcc tcaactccgc gactatctcc gccgcgcgct cgacatgtcg cggcacgatg
     2701 atgagcgaca gaccgccgaa cttttctcga agccgcagcc acatgccggc cagcattttt
     2761 tcctcccccg gatgcgtgct ggccagcagc agcaccggcc gggagccgcg gatgccgcag
     2821 cgctccatga ccgcgcgcaa agattcaacc tgcgccgcgt ccgcctccgc gccctgcggg
     2881 tcaaatttga tgctgcccgt gagatgcgcg cggccttccc tgacaccgag tccggcgaag
     2941 cgctcgcgat cccccggctc ctgcacgcag accaggtcca gcaagcggaa aaccggccgg
     3001 atgaaaaagc cgaaccggcg gaaacgccgc tcggagcgcg cggacaggcg cgcgttcacc
     3061 aggctcatgc gcacacccgc gaggcatgcc gccgcggcaa ggttcggcca aagctccgcc
     3121 tccacgagaa tgatctgctc cggtctcgcc aggcgcaaag gccacgcccc gatcagcggc
     3181 aggtccaccg gcgagtagag caccaccacc cgccccgcat gacgcgtggc gaactccgcg
     3241 gccagcgcgt ggccggtcgg cgtcgtcgtg gtcaggagca cccccaggtc cggcgcgcga
     3301 gccagcagtg ccgggatcag cttcatggcg atgcccgcct cccccacgct caccgcgtgg
     3361 acccagacac ggcgcggcgg cagggcgtcc agcgcggcgc gtttatccgg cggaagaaat
     3421 ccgagacgct gcgccaggtc ctgccagtgt ccgccgcgtc tgcgcatctt ggcaagcgcg
     3481 cccggcagca tcgccagcag ggcggcgggc agcagcagat tataaatgag caggctgaaa
     3541 aggaggcgca tgaatacggt caagctcaag cagccggcgg cgcgcgggtc aaggctggat
     3601 cgcgcgctgc agccgccggt tgcgccgccg gtgcggggtg ttgcatccgc gataggtgag
     3661 agtgtctcaa gaacgcgcaa caaggcccgg cttcccgcgt cttcattccc atgcgccctc
     3721 tccttctctg tctcatcgcc atgcattgcg cccttggctc caccggccgg ccgcccaatt
     3781 tcgtgatgat tttcatagat gacatgggtt ggggcgacct ctcctgcttc ggcaacgagg
     3841 acgccgccac gcccaatctc gaccgtctcg cgcgggaggg cgtgcgcttc gagcatttct
     3901 atgtcaactc gcccatctgc tccccgtccc gctgcgctct caccaccggg cagtatccgc
     3961 agcgctggtc catcacctct ttcctcaaca atcgcgccga caatgaccgc cgcggtgtcg
     4021 cccagtggct cgacccgcgc gccgccaccc tgcccggcat cctccgtcaa aacggctacg
     4081 ccaccgggca cttcggcaaa tggcacctcg gcggccagcg cgatgtggac gacgccccgc
     4141 ccattacagc ctatggcttt gacgaaagcc tcaccaactt cgaaggcatg ggcgccaagc
     4201 tcctgcccat gacgctcaag cccggagaca ccgccccggg acgcatctgg caggacgccg
     4261 agcgtctcgg cggaccggtc gtctggatgc ggcgcagcga gatcaccgcc ggctttgtca
     4321 acgcggccat ccctttcatc aaccgcgccg ccgccgccgg caaaccgttc tacatcaacc
     4381 tctggcccga tgatgtccac ggcccctggt ggccgcccac gagtcaatgg ggggacggct
     4441 cccgccgccg tctctacctt tccgtccttg aggaaatgga ccgtcagttg ggccccttgc
     4501 tggagcatat ccgcgccaca cccgccctcc gcgacaacac cctcgtgctc gtctgctccg
     4561 acaacggcca cgaaacggga gccggcagcg gcggcccctt ccgcgggcac aaggccacac
     4621 ttcatgaagg cggcatacgc tccccgctca tcgcatgggg gccgggcctc atcgccgaat
     4681 cccgggccgg aaccaccaac accacttccg tctttgccgc cttcgacatc gctccctccc
     4741 tcctcgccat cgccggcatc gcggcaaacg cgggctccgg tgtcgcgcct tcgagtttcg
     4801 acggcgagga tatttctccg cttcttctcg gcaggagcac cgtttcgcgt cgtgctccgc
     4861 ttttctggcg gcgaccgccc gaccgcaagc acgcctatgg ctccggcccg ctgccagacc
     4921 tcgccgtgcg caaggatgaa tggaagctcc tttgcgactt cgacggctca aactccgggc
     4981 tgtaccatct catggacgat cccggtgaaa tctccaatct cgccgcgcgc catcccgaca
     5041 tagccgaccg cctcactcgc gccgtgctcg actggaacgc ctccatgccc aaggacaacg
     5101 gccaggcgct cggcaaggaa gcgccgcccg cgcccaagaa gaaaaaaaag tgacattcca
     5161 agccatgccc gggccgtgat cgcggccttc atccgccaga ctcgcccaac ctggtgaggt
     5221 gaggcatggc cgtgaaggtt tttcgaattt cgaatttcga actccgcatt ttctctctat
     5281 gggatgccgc gcatggaatc gccctacgcc ccgcctcccg ctgatgaacc cgccgcagag
     5341 ccccggctcc cagcggccac cgccgtcttc cagcgcattc gcgacagcat cgcgcgggtg
     5401 tttgtcgggc aggacgaggt catccaccag gtgctcgcgg ccctgctggc gggcgggcat
     5461 gtcctcctgg aggggaagcc gggccttggc aagacgcatc tcgtgctggc gctctcgcgc
     5521 acgtttggcg cggcttttcg ccgcatccag ttcacgccgg acctgatgcc ctcggatgtc
     5581 accgggcaca cgctatacga cctgggcagc cagagcttcc gcgtgcgcaa aggccccgtc
     5641 ttcacccagc tcctgctcgc cgacgagatc aatcgcgcgc ccgccaagac acagtccgcg
     5701 ctgctggagg tgatgcagga aacccaggtc accattgacg gcgagaccca cgcgctgacg
     5761 cctcccttca tgaccttcgc cacgcagaac cccattgagc aggagggcac ctatccgctg
     5821 ccggaggcgc agctcgaccg cttcctgctc aaggtcctca ttgactaccc cgatgccgcc
     5881 gaggaggcgg ccatcgtcaa agccgtcact gccgaagcgg gcgggcgcgg cctttctccc
     5941 gcccatttgc agcccgtctg ctccaccggt gacatcctcg ccgcccagcg cgaggcagcc
     6001 gccgtggagg cggtggacag cgtggtgaat tacgcggtgg aacttgtgcg cgccacgcgc
     6061 aagcacggcg ccattgagat cggtgcgggc acgcgcggcg ccatcagcct catccgcgtg
     6121 gccaaggctt gcgccctgct tgacgggcgc gcctacatca ctccgggtga tgtgaagcgc
     6181 gccgccctgc cggtgctgcg gcaccgcgtt tccctgacgc cggaggtgac catcagcgga
     6241 caagatgtgg accaggtgct tgagacggtg atccgtggag tcgaggcgcc tcgcgcatga
     6301 ttccacgccc gcgctgcctt acggcttcac gaaccggtag tgggagacaa accactcggc
     6361 gccgtccttc cagccccaca gctcggcgca ggccatgaaa aagatgcgcc agtaggcccg
     6421 ccatttcaca gcctggtccg cgccataggt ctcgcggaaa agcgtcatga tctccgcctt
     6481 gttcgcgtcc atgcgcgcga gccatgcctc tgaggtgcgg ctgtaatgcg tgccgttgac
     6541 gatccaatgg tcctcgatct tgagatcgtc ctggaagtag agcagcaagt catccgaagg
     6601 catctggccg ccggtgaaga agtggcgcgc catccagtcg ctctcgttcg cggcctcgta
     6661 gtggtaggcg aactcgcggt gggtgaatat gtggacgaag agcctgcctc ctggcttcag
     6721 ccaggaggcg atgcggccca tgagttcctg gtagtttttc atgtgctcaa acatttccac
     6781 cgagacgcag cggtcgaaga cgccctcacc cgcgccctgg tagtgaatca tgttcgcggt
     6841 gacgacctcc agattggcca gtccgcgccg ggcggcctcg gcgtcaatgt gcgccttttg
     6901 cgtgcgcgag ttggagacgc tggtgatgcg cgagtttgga taatgctccg ccatccacaa
     6961 cgagagcgat ccccagccgc agcccagctc caggacgcgc tgcccatcct gcaacgccgc
     7021 tcgctcgcag gtcatgcgca gcatgagggc ctccgaatca tcaaacgtgg tgccgtgttc
     7081 cggccagtag ccgctgctgt atttcaaatg cggaccgagg cagagctggt aaaagcgcgt
     7141 gggcacctcg taatgctgct cgttggccgc gtctgtctcg acagccaccg gtccggcctt
     7201 caaccccgcg atgtggctca tgagcgcggc ctggcgcgcc tctgggtcca ggctttcatg
     7261 cctgcgcaga gtggcggcga ggcgctggcg gattcccaaa cggatggccg cgtccggcag
     7321 caggtctttg gcgaggaggt cgttgaggtt cagcatcgga ataataggtc ggcagctcag
     7381 gcggcgcggc ggcgcaggag cacatcctcc aggcgcgcat cgaggggaac gcggagtagc
     7441 tggatgagcg cgtagatgga gatcgcgatg tttcccagca gcaggatgcc aagcagccag
     7501 ccgaggcgcg aggtcagggc tgtctccttg taaaagaccc agcaataaaa agccaggaag
     7561 ccccaatagg cgtcgaacag cgtggcgatg aaccagggat gcgcggccac cccgcgcggg
     7621 atggcccaca aggcgcactg gccggaggcc catgcggtga ccgccagcat ggagacgagc
     7681 acggtgacaa agaagactcg cagggccagg atcatggggt tggattcttc ttcaaaccgg
     7741 tctcaccagg cacggtgcag gctttcattg ttcgggcggg tatagaccgc ctggatgacg
     7801 ctgatgttcc gcgtggcgaa ggccgcctcg cagtacttca agtagtagct ccacttgcgg
     7861 atgaacgcct cgctgaagcc catgacgcgg acctcgtcga gcctcgcgtg aaaagcgtcg
     7921 tgccagagct tgagcgtgcg cgcgtagccg gaaccgagat cctccagcgc gtgcatgaac
     7981 aaatcgctcg tgcggttggc ggcccggttg agctggttca cgctgagcag cagggagccg
     8041 gggaagatgt gcttttggat ccagtccacg cccttgcgca acgccgcatg gcggcagtct
     8101 ggcgtggtga tggcttggaa tgccagcaag ccttcgggtt tgagcagctc gtggcacttg
     8161 gcgaaccagg tgtccaggaa ctcgcccccg acggcttcca gcatctcgat ggagcagatt
     8221 ttgtcgaacc tgccttcgac atgccggtag tcctggagct ggatggtgac gcggtccgcg
     8281 agaccctcgc gcagaacgcg cgcggtggcg taatcaaact gctccttgga gattgtcacc
     8341 gctgtgacgc ggcagccatg ctcgcgcgcc gcgtgcgtgc agaagccgcc ccagccgcag
     8401 ccgatctcca gcacatgatc ctccggcttg agctggagct tccggcacag cgcctcgtat
     8461 ttggcgcgct gcgcgtcttc cagactctgc tccgcgtgct cgaaccgcgc ggccgagtaa
     8521 gtcatcgtcg ggtcgagcca gagcgcgtag aagtcattcc ccaggtcgta gtgctcggag
     8581 atgttgcgcc ggctgttgga ctcatggttg cgccgtccga agtgccaaag cctgctgccc
     8641 aggcgcagca agttggtgaa tggcagcctg ctggacgagg cgcgcgcgcc ctggctgcgg
     8701 gtgatgctgc ggatgaacca ggtgatgacg gcggcgatgt cgtccgtgtc ccattcaccc
     8761 gcggtgtggg actcgccaaa gccgatgttg ccaaacaaaa tgcagcggcg gaaaaaggcg
     8821 cgcgggtctc gcaggcgcat ctccgcctgg ggcgcggaga cgccgtcacc gagaaggcgg
     8881 acgcgtcccc cgggatagac gatgcgcagg cagccgtgcc gcattgagcg catggcgaag
     8941 aagaacagcc tctcgtaaaa tccaccctcg gcggaggatg cggtggcggg tattgcctcc
     9001 gcctgggcgg agtcgagcgt cagcgtggag ttcatgtctg cgtgggagcg atggagcggt
     9061 gcgggcggta aacacccgtt tggagatgcg ggtcggcagc cttgcggtga acggccagtt
     9121 ttttcatcca cagccgcagg gcgtgccagt gaatgagaaa gacgaccctc agcgtgagca
     9181 gcggatactt tacggcgcag gccagcaacg aggcgtcggt gagttcgcgc cgtttgccgg
     9241 tgaggacgct gagcagcacg agtccggcgt cgtcgcgctc atccacatgg atttcgagag
     9301 cctcgcccgg caggcgcagt ttgaagtcga attccagatc caaaccggag aaaggcgaga
     9361 cataaaaatg cttcggcgtg acgaggcgaa aacggtcttt ccagtccatg cccgtcagca
     9421 catagggctt ctgttcgtga aaggtgttcg tgacctcggc caccgcgcac acgggatttt
     9481 cctccgcgtc gtaggcatag taaaagcaga cgggattgaa gatgtgaccc agcacgcgcg
     9541 gaaaggtcag cagccgcacg cgcgcgactt cctccgtgtc cactccggct tcgcgcatca
     9601 cggcgaggat gttttccctg gcgctggccg ggccttttgg gatgtggtcg gagtcgaaga
     9661 aggaaaacaa gctccaacgg ttccttttga aaagtgccag actttccgag aaagcgtcca
     9721 gttcgtcgag atcaagccag agataaaata ctcggtaggc aaaatggtgc tcctttggcc
     9781 tcagccggcg gtgcgtcacc tcgcactcat agagggcgga gaatgggacg gtgctggagt
     9841 ctggggcggg cactcccggc atttcgtccg ccgcgtggat ttggattcaa aattgcggga
     9901 aggactcccg cccaggttgt tccgtggcgc gccgcgccct cgtgatcact gacatccaag
     9961 gttgcgcaaa tcttcgcccg cgccttcttc cgttccctca tatgtccagc gagcagcgca
    10021 aagccttccc tttctccgag tttgaaccga agtggcaggg cgagtgggag gccagcaagg
    10081 cctaccgcac gcctaacccc ggtgatgccg acttcgatgc ctcgaagccg aagtacttcg
    10141 tgctcgacat gttcccctat cctagcggca acggcctcca cgtcggccac ccggagggct
    10201 acaccgccac ggacatcatc ggccgcttca agaagatgaa cggcttcaac gtgctgcacc
    10261 cgatgggctg ggacgctttt ggcctgcctg cggagcagta tgcgatcaaa accggcacgc
    10321 atccccgcgt gaccacagag gcgaacgtcg ccaacttcac caagcagctc aaatcgctcg
    10381 gctttgccta cgactgggac cgcgaggtga acacgaccga ccccggctat ttcaagtgga
    10441 cgcagtggat cttcctcaag ctctacaact cctacgtcgg cgacgacggc aaggcgcatc
    10501 cgatcagcga actcgaagcc cagggcctct cacgcgagga gatcgaccat cgccgcctcg
    10561 cctacgtcag cgaagcgccg gtgaactggt gcccgcaact cggcaccgtg ctcgccaatg
    10621 aagaagtcgt ggacggcaaa agcgaagtcg gcggtttccc cgttgagcgc cgccccatgc
    10681 gccagtggat gctgcgcatc acgtcgtatg cccagcgcct catcgacgaa ctcgacggcc
    10741 tcgactggcc ggaaagcatc aagctgctcc agcggaattg gatcggccgc agcgagggcg
    10801 cgcaagtgca atttcggatt gcggattgcg gattgccgaa tgacgagacg atcaccgtct
    10861 tcaccacacg gcccgacacg cttttcggag ccacctacat ggtgctcgcg cccgagcatt
    10921 cgctggtgga tgccattgcc gctgcggaat ggcccgcgaa cacacctgac acctggaagg
    10981 gcgacccatc cgcaatccgc aatccgcaat ccgcaattgc gtggtatcgc gaaaagaccg
    11041 cgcggaagag cgatctggag cgcaccgagc tggcgaagga gaaatccggc gtcttcaccg
    11101 gcgcttacgc catcaatccg gtgaacaacg agcgcatccc catctggatc gccgattacg
    11161 tcctcatggg ctacggcacc ggagccatca tggccgtgcc cgcgcatgac gagcgtgact
    11221 ttgagttcgc gaagaagttt gagctgccga tcaagcaagt ggttgcggca gcggggaaag
    11281 catggggaac aggcgtctcg cctgttcagt ctcagagcac aggcgggatg cctgtgcccc
    11341 atgctgtgcc tcatgctgtg cctcatgctt tgcccagcag caaatacctc aacgaccgca
    11401 ctgagatcga aaagaagcgc cgcaacctcc ctcactggca gcaggatgga aaacaatact
    11461 tccttacctg gcgtctggct gacagcctgc ctgtggaaaa attgcgcgag ctgcatgacc
    11521 tgcgccagca ggcatcacta ctgccagagg atcagaggca ggagcatgat gcccggatct
    11581 ttgaaacaac aagcgcatgg ctggatgagg ggcatggctc ctgtattctc aaacggcctg
    11641 agctacgcca gatcgttacc actgcattct ctcactttga cggcaaacgc tacgatctgg
    11701 acgcttatgt ggtgatgcca aaccatgcgc atgtgctcgt gacattgaag ccgggagagg
    11761 atttgtccaa aattctgcat tcttggaaaa gctatacggc caaggagatc aataaactgc
    11821 tgggtgagga gggcgctgtc tggcaggatg agaactttga ccggctgatc cgcagcagag
    11881 agcacgagtg gtttgttcgg gattacattt ggaaaaaccc ttcagaggca ggtctgcgtg
    11941 agggggagtt tgattggttt gagagaacag gcgagacgcc tgttccccat gctgaaacac
    12001 tgcccgagtc tgcattctgt gaaaacggca ttgccgtgaa ctccggcttc ctcgatggcc
    12061 tgcccactcc cgaagcgaag aagaaaatca ccgtatggct ggagcagaat ggtttcggca
    12121 aaggcacgac caacttcaag ctgcgtgact ggctcttcag ccgtcaacgc tactggggcg
    12181 agccgttccc gatcgtctgg gagaacggcc agcatcgcag ccttcctgaa agtgagctgc
    12241 cgctgctgcc gccgacgttg gaagacttca aacccaccgg cacgcccgaa cctccgctgt
    12301 cgaaggccac ggagtgggtc caatactccg ccaccgcgaa gcgcgagctc aacacgatgc
    12361 cgcagtgggc gggttcgtgc tggtattacc tgcgttattg cgatgcgcgg aacagcgagc
    12421 gctttgtcgg cgaggccgcg gagaagtatt ggatgggcgg cggcaagccc ggcggcgtcg
    12481 atttgtatgt cggcggcacc gagcacgccg tgctgcacct gctttacgca cgtttctggc
    12541 acaaggtgct ctttgatctc ggctacgtca gcacgccgga gccattccag cgtctggtga
    12601 atcaaggcct catccttggc gaactcgaat acacgctgta tcgcaagcca gacggaaccc
    12661 cgctttccta cgtcgcgaac tacatgcccc aattggctga atcactccgt tgggaaactt
    12721 tagatcccga agaagtggaa cggtgcccgc aaggcttggg ttatgtttgg aagcaagaca
    12781 agtcgatcta tgttgaagcg aaatcacata agatgtcgaa aagcaggggc aatgtgatta
    12841 acccagacga catcattcgt gactatggtg cagacgcctt gaggttgtat gagatgttca
    12901 tgggcccgct ggagcaggtg aaaccctgga gcacagccgg tgtagtcggg cagtctcgct
    12961 tccttgcccg catatggcgt ctggtgatgg aagtcgatga cgaaggcgtg tggagccttt
    13021 ccaaggccct gcaagacgtg cctgcgaaca agcagctcac caaagcgctg cacgagacga
    13081 tcaagaagtg cggcgaggac attgagaagc tcagcttcaa caccgccatc agtgcgatga
    13141 tggtttgcac cacagccttc accggagctg aagtgaagcc cgcggcagcc atcgtgacct
    13201 tcctcaaggt gctcagcccc ttcgcgccgc atctcgccga agaactcaac gcgcagatcc
    13261 gcgccaaatt ccccgccttg gccgcgaaac gcttcctcag cgacgaaacc tggcccgcct
    13321 acgatcccgc cgccctcatc gaggacgagg tcgaggtcgt cttccaggtg aacggcaagc
    13381 tgcgcgacaa agcccgcgtg ccgatccagg ccacaaagga ggagatcgaa aaaatcgccc
    13441 tcgccagcgc ccgcgtgcag gaa
//
LOCUS       DAOMYJ010000139        13383 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Prosthecobacter sp. isolate AMR_MDS_5629
            NODE_1544_length_13383_cov_22.329907, whole genome shotgun
            sequence.
ACCESSION   DAOMYJ010000139 DAOMYJ010000000
VERSION     DAOMYJ010000139.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867850
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prosthecobacter sp. (wastewater metagenome)
  ORGANISM  Prosthecobacter sp.
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Verrucomicrobiaceae; Prosthecobacter.
REFERENCE   1  (bases 1 to 13383)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13383)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.30
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:34:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,742
            CDSs (total)                      :: 4,684
            Genes (coding)                    :: 4,676
            CDSs (with protein)               :: 4,676
            Genes (RNA)                       :: 58
            tRNAs                             :: 55
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13383
                     /organism="Prosthecobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1544_length_13383_cov_22.329907"
                     /isolate="AMR_MDS_5629"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1965333"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1328
                     /locus_tag="PLP58_14245"
     CDS             <1..1328
                     /locus_tag="PLP58_14245"
                     /inference="COORDINATES: protein motif:HMM:NF012581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="proton-conducting transporter membrane subunit"
                     /protein_id="HRJ09734.1"
                     /translation="IHVVQPGGWPFPLGISLVGDALSALMVAVCALVGLAGAVYLAGE
                     KREDIEARGLHPLLLILLGGVNGAFLTGDAFNLYVWFEVMLIASFVLLGLCGGRASVE
                     ASVKYVIINLAASILFLIALGLLYGKAGTLNFADLHEKLGAPAAAAGTQSAAVLMLVA
                     FGIKAGAFPLFMWLPASYPALPAALGAVFAGLLTKVGVYAMMRVFHLVFAVHEAWMQP
                     VLWWAAALTMLVGVFGAASQMEIKSVLSFHIVSQIGYMVAGIALGTRAALAATVFYIV
                     HHILVKANLFLLGGVIGQRCGGTGLKHCGGLYASAPWFAFLFLIPAMSLGGIPPLSGF
                     WAKYSLLREALTLGDGWITFVALATGLLTLYSMTKIWKEAFWKSPPADMPAPGPWPRW
                     SLAACLLLAAATVALSLHPQPLLEMAQNAARALFEPALYVEAVKKGGAP"
     gene            1325..1660
                     /locus_tag="PLP58_14250"
     CDS             1325..1660
                     /locus_tag="PLP58_14250"
                     /inference="COORDINATES: protein motif:HMM:NF014012.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter subunit E"
                     /protein_id="HRJ09735.1"
                     /translation="MILLRWLCFLLTYTWDVARSSLQVARDVLSPRPRIAPAFVRVPL
                     RPAGDLKLLLLSNLITFTPGTVFVDVSPDARECVIHTLYGGDSGENVARLSRDIARLQ
                     DQLDLLLPS"
     gene            1657..1965
                     /locus_tag="PLP58_14255"
     CDS             1657..1965
                     /locus_tag="PLP58_14255"
                     /inference="COORDINATES: protein motif:HMM:NF015993.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015075 - monoatomic ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="monovalent cation/H+ antiporter complex subunit
                     F"
                     /protein_id="HRJ09736.1"
                     /translation="MNLEEITTQLCGLMLGTSVLLIFWRFWRGPTDADRVLAIDLAAV
                     VIAAAMIVNMVRSGEAVFLDAILLMGGVLFFSTIAFARALEVRNQKRRIREASHDPRP
                     "
     gene            1949..2257
                     /locus_tag="PLP58_14260"
     CDS             1949..2257
                     /locus_tag="PLP58_14260"
                     /inference="COORDINATES: protein motif:HMM:NF015302.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monovalent cation/H(+) antiporter subunit G"
                     /protein_id="HRJ09737.1"
                     /translation="MTLALEILKDLLLLAGAFFVLVSAIGLARMDGFPARMQAAGKAA
                     AMGLGLLLAAVALDAGTWVVALKGVAAMVLLFLTLPIASHALMRTWWRQEGGGDDTAR
                     "
     gene            2330..3658
                     /locus_tag="PLP58_14265"
     CDS             2330..3658
                     /locus_tag="PLP58_14265"
                     /inference="COORDINATES: protein motif:HMM:NF013741.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HRJ09738.1"
                     /translation="MTGIFFELGLLFVLLLANGVFAMAEIAVVSSRKARLQTRVNAGS
                     SGARTALELLEDPGRFLSTVQVGITLVGTLAGASSGAALIARLTPVLAQAPFLSEYWA
                     GMLATILVVSFITFLSVVIGELVPKRLAIHSPEDAAARLSPPMNLLSRLAGPVVRLLD
                     ASSGLLARLLGVRPGEEPGVSEDEVRALMHQGTLSGVFKPGEQRMVEGVLELDDLVAA
                     DVMTPKNQIIWVDLDEPDDENEKLMAESGHTHFPAYRDTRDNVLGMISVKALWAARRG
                     ASAGKTLESLLTEPVFVSETMPCPRIIEEFRRRRRHLALVADEFGGVSGLVTLHDLME
                     SVLGSMPDLDQRAKPQVRQLDDGSWLVDAMIAIEDAAARTGFTVPQDEIDEGLYRTLS
                     GFLLHRFGHVPQEGEKLQHDGFEFEITDMDRQRMDKVTIRGKAAVPAQAA"
     gene            3838..5742
                     /locus_tag="PLP58_14270"
     CDS             3838..5742
                     /locus_tag="PLP58_14270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose oxidase"
                     /protein_id="HRJ09739.1"
                     /translation="MKRKFIIQSLAAMLLAVAMPMTAQVPQLIQFQGRIAVAGTNFDG
                     SGQFKFALVNADGSTTYWSNDNSSTAGGQPAAAVTLPVVKGLYSVLLGDATLPNMTVV
                     PSSVFNNGDVHLRVWFNDGVNGFHHLTPDQRIAAVGYAMMAANVPDGSITGAKIAPGA
                     VGASQLAPDAVMGSLDAAGVGFVPSGGIILSDQENNTALLDAGYVKINGTVPKPAWTE
                     LAESEIFRYEGETVIWTGSEVIYWGGTDLFFGDITNSGERFNPSTNTWTPMSDEGAPF
                     PRPFDEVLWTGSQMIVWGGEDIDLGGPANTGAIYDPEADTWTEMSTEGAPLLMFPEVF
                     WAGDRMIVWGGWDPIDGIPTNTGAMYDPETDAWTEISDVNAPVARDSAIILWTGTEMI
                     VWGGFDIDGFPDDTGARYNPSTNTWTTMSFVGAPALMYGFENPPVVFWSGTEMLIWGL
                     SGFDEDLGFPIYAGGRYNPATNTWAAISTEDAPEPFSFDAYDFKPRTAWTGTELIIWC
                     GKDNDGGARYNPATDTWTPISLNQAPDQTSGNSRTIWTGTELLNWGGWFNLSSEYNSG
                     SRYNPETDTWKKISSTDAPFAYDPVPSVVWTGSQMIVMPHIDPGLGAPEPAFSYTPSE
                     SDFFFIYQRP"
     gene            5739..8396
                     /locus_tag="PLP58_14275"
     CDS             5739..8396
                     /locus_tag="PLP58_14275"
                     /inference="COORDINATES: protein motif:HMM:NF037015.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HRJ09740.1"
                     /translation="MNSRRLLFSLSLACAVVTGLRAETRTGGPYQLHADHVGSGGGKA
                     AGSIYIQDSTLGSIGGTSNADGMLSKVGYIAQLYEVVSLEITAAPATVDERGTRQLAS
                     TALLDDGTLLANLAGSTTWSVLSGPITGISSGGLASAGTVSENTLASVQGSWSGFSDT
                     LDLTVLDIFDDRTPVSLAANLHTVFQGASSVEITLTRPSGEGPASVTLAASNGTNSAM
                     PPFTGGVVGKDYQALNTVVQFAANELVKTQTVTLIPRTGKVPNIRFNVALNSPGPGAT
                     LGAITQAEIQILANDTTKPTLTLKTPAAGTVSSVLPVLLTGTVGDALGIERLEYSLNG
                     STPEDLMLGASTKATAIPFSAEIEPQEGANSLTLTAYDLRGNSTTLVRDFIFERRYVL
                     TLLRNVPAGVAAEPDKAGTLALAAVPTKAASTLTKGPAPQTSHVVHGSQVTVTASAKT
                     GHILSHWTGLPAGAQTLGNVVSFAMPEADVPGLTAVFVANPFPALVGTKKPVYQGLLE
                     PDGMTPANNGTVGMVTGTLTPAKGTLSGKIWLDGKSTGFVAHLHGNGSVWFLVEKKLS
                     TQFPFLGRSLEMSWSDDGLDMEVSGPAGTSEGVAKMPAYSKTQTVGAGLLDALGKQGY
                     YTVALPAKEQSPAKDTDTYPQGTGYKTLTLLADGTFKLAGVLADGTKITAASYLVEGD
                     EAAVFIQLPIPSTSAKDGSFSGMLVFDPANDDSDVSALDMRWYRPAVVEGTTVATQLY
                     TAGWPDGVVLDAVGALYDKNLDFQTVLALGAPDVDGNAALIFKDGKLVTPPDLLVVPD
                     INITLSKVAKIPATNKTFTLTLTQAKGAFNGTFTPNWTQENKTLPKFQGVVLQKGANK
                     GGYGYFVSNRLTDLDPESGSVALKASSGL"
     gene            complement(8516..9880)
                     /gene="glmM"
                     /locus_tag="PLP58_14280"
     CDS             complement(8516..9880)
                     /gene="glmM"
                     /locus_tag="PLP58_14280"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: protein motif:HMM:TIGR01455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008966 - phosphoglucosamine mutase
                     activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HRJ09741.1"
                     /translation="MSDKRQFFGTDGVRATANRHPMTPEFVLRLGQAAARVLTAGHEG
                     HERPRCVIGRDTRASGEMLEAALIAGLNSVGVDAVLAGVLPTPAVAMLAARTGADFGI
                     IVSASHNPFEDNGIKFVHGNGRKLNDATESAIEKIVLGSDTEPARPEGRGIGRVSRLA
                     DSVELYVAHAVASMGGTRLDGMRVALDNAHGAASLTSAEALERLGADVQVFHSEPDGF
                     NINEECGCTHSEELERLVRQSQAQAGIAHDGDADRIALCDEEAIALDGDELMAVAAES
                     MLRKGTLKQNILAVTIMSNYGLDDLVSRLGGRVIRTNVGDRYVLEQMHERGLNLGGEQ
                     SGHIIFGDWATTGDGLIAGLQILKIMKETGEPLSQLRKCLKKFPQSSRNLRVRSKPPI
                     TELVEAQKIIKETEKKLGDYGRVLLRYSGTESLIRLLVEGRDVEYLEAQADKIASAVL
                     AQIG"
     gene            complement(9914..10936)
                     /locus_tag="PLP58_14285"
     CDS             complement(9914..10936)
                     /locus_tag="PLP58_14285"
                     /inference="COORDINATES: protein motif:HMM:NF012798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HRJ09742.1"
                     /translation="MEASEKLAPYGKTLREALDFYLAHLGRVQRPCTVAELVDDLLTL
                     KGREKKSQRNQNDLRLRLERFARDFEGRQVASVTADEISSWLHDLNLSAVSVSNYRRV
                     LGVLFSRALSMGLSERNPVAASMKPNEIEGEIGILTPGETARLLAFAHDEIRAVIALG
                     LFAGFRHAELMKLDRREVDLRGGNATVKAAKAKSARRRIILIWENLAAWLAPVAKLAG
                     SVWPEKHQRGRTLFEEARRAAGSGDEGSETPEEKAAGLKLKPWPHKVMRHSFASCHLA
                     KWRNAASLALELGHTGTALIFSNHRELVRPDEGAACWNISPSNGRRCGASWKAWHECE
                     VASPPL"
     gene            complement(11102..12121)
                     /locus_tag="PLP58_14290"
     CDS             complement(11102..12121)
                     /locus_tag="PLP58_14290"
                     /inference="COORDINATES: protein motif:HMM:NF015131.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome-c peroxidase"
                     /protein_id="HRJ09743.1"
                     /translation="MKKTTLHSLMLAAFSAATVHADAFSDSYLAMFRPLPAEAPSPQN
                     ELTEAKINLGRMLYYEDRISKGGKISCNSCHQLDKYGQDNLPFSPGHDGSLGGRSSPP
                     TYNAALHIAQFWDGRAPTVEEQAKGPVLNPIEMGMPDADFVVKVLKSIPGYVEAFKAA
                     FPGEADPVTYDNFGKAIGAFERKLLTPAPWDDYLKGRKDALTAEQVKGYETFAKSGCV
                     TCHNGPAVGGSMYQKLGLVKPWPELKDLGRFEATKQESDKHFFKAPSLRNITETGPYL
                     HDGSVKSLEEMVKKMASHQLGKELTDEEVSSIVTFLGALKGRIPADYIAKPALPPSGP
                     DTPKA"
     gene            12282..12977
                     /locus_tag="PLP58_14295"
     CDS             12282..12977
                     /locus_tag="PLP58_14295"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HRJ09744.1"
                     /translation="MFARFLIALSLAAAAMLAAQQGSAPLREVIAKLMEAQDLKNAGK
                     FTDALEKLDEVEALAPDTPHLPNLRGTIYLAPQLRDLDKAEELFTRAIELNPGDYIPR
                     FHLAEVSYVRHDWQAARAAFQKLLDDYPKTPMAIRHMVVFKRLVCEVKLDRFDEAEKT
                     LKEHFTFMDDTAAYYYGHAAIAFGRRDEAKAKEWIGRAEGIFKKEDRSTYEDTLMEAR
                     WLPNIGLPPLEKR"
     gene            complement(13025..>13383)
                     /locus_tag="PLP58_14300"
     CDS             complement(13025..>13383)
                     /locus_tag="PLP58_14300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ09745.1"
                     /translation="HRHRLHHLRHALARHPLPGAVRVSSRAASTGASSSVVRSSNIGA
                     VAKAASRCSTAPVIPSTRRCSPPARAENPAQKSAGISGRPATSTSPPAARATRTRSTP
                     EESTLQRASRPSGSSA"
BASE COUNT         2289 a         4375 c         4141 g         2578 t
ORIGIN      
        1 gcatccatgt ggtgcagccc ggcggctggc cttttccgct gggcatcagc ctcgtgggcg
       61 acgcgctcag cgcgctcatg gtggccgtgt gcgccctggt cggactggcg ggcgcggttt
      121 atctggccgg ggaaaagcgc gaggatatcg aggcgcgcgg cctgcacccg ctgctgctca
      181 tcctcctggg cggcgtgaac ggcgccttcc tcaccgggga cgccttcaac ctgtacgtct
      241 ggttcgaggt catgctcatc gcctccttcg tgctgctcgg cctctgcggc gggcgcgcct
      301 cggtggaggc ctcggtgaag tatgtcatca tcaatctcgc cgcctccatc ctcttcctca
      361 tcgccctcgg cctgctgtat ggaaaggcgg gcacgctgaa cttcgccgac ctgcatgaaa
      421 agctcggcgc tcccgcggcc gcggccggca cgcaaagcgc cgccgtgctc atgctcgtgg
      481 ccttcggcat caaagccggc gccttccccc tcttcatgtg gctgcccgcc tcctaccccg
      541 cgctgcccgc cgcgctgggc gccgtcttcg ccgggctgct gacgaaagtg ggcgtgtatg
      601 ccatgatgcg cgtcttccac ctcgtcttcg cagtccatga ggcatggatg cagcccgtgc
      661 tgtggtgggc ggccgcgctc accatgctcg tcggagtctt tggcgccgcc tcgcagatgg
      721 aaatcaagag cgtgctgtcc ttccacatcg tcagccagat cggctacatg gtcgccggca
      781 tcgccctcgg cacccgcgcg gcactggcgg ccaccgtctt ttacatcgtc catcacatcc
      841 tggtgaaggc gaacctcttc ctgctcggcg gcgtcatcgg ccagcgttgc ggcggcaccg
      901 ggctgaagca ctgcggcggc ctctacgcca gcgcgccgtg gttcgccttt ctctttctca
      961 tcccagccat gtccctcggc ggcatccccc cgctctccgg cttctgggcc aaatactccc
     1021 tgctgcgcga ggcgctcacg ctcggggacg gctggatcac cttcgtcgcc ctcgccaccg
     1081 gcctgctcac cctctactcc atgacgaaaa tctggaagga agccttctgg aaaagccctc
     1141 cggcagatat gcccgccccc ggcccctggc cccggtggtc cctggccgcc tgcctgctgc
     1201 ttgccgccgc caccgtggca ctctccctcc atcctcagcc gctcctggag atggcccaaa
     1261 acgccgcccg cgccctcttc gagcccgccc tctatgtcga agccgtgaag aaaggaggcg
     1321 cgccatgatc cttctgcgct ggctctgctt tctgctcact tatacctggg atgtcgcccg
     1381 ctccagcctc caggtcgcgc gggacgtgct ctcgccgcgc ccgcgcatcg cccccgcctt
     1441 cgtgcgcgtg ccgctgcggc ccgctggtga cttgaagctg ctgctgctct ccaacctcat
     1501 caccttcacc cccggcacgg tcttcgtgga tgtctccccc gacgcccggg aatgcgtcat
     1561 ccacaccctc tacggcggcg actccgggga aaacgtcgcc cgtctcagcc gcgacatcgc
     1621 ccgcctgcag gaccagctcg accttctttt accctcatga acctggagga aatcaccacc
     1681 cagctctgcg gcctcatgct cggcacctcc gtcctgctca tcttctggcg cttctggcgc
     1741 ggccccacgg atgccgaccg tgtgctcgcc atagacctgg ccgccgtcgt catcgccgcc
     1801 gccatgatcg tgaacatggt ccgcagcggc gaggccgttt ttctcgacgc catcctgctc
     1861 atggggggcg tgctcttttt cagcaccatt gccttcgccc gcgccctgga ggtgcggaac
     1921 caaaaacgcc gcatccggga ggcctcccat gaccctcgcc cttgaaatcc tcaaagacct
     1981 gctgctcctg gcaggtgcct tcttcgtcct ggtctccgcc atcggcctcg cgcgcatgga
     2041 cggcttccca gcgcgaatgc aggccgcggg caaagccgcc gccatgggcc tcggcctcct
     2101 gctcgccgcc gtcgccctcg atgccggcac ctgggtcgtc gccctgaaag gagtcgccgc
     2161 catggtcctg ctctttctca ccctccccat cgccagccac gccctcatgc gcacctggtg
     2221 gcggcaggag ggtggcgggg acgacacggc gcgctgatgc gcatccgccc ggcaatacag
     2281 cgaccatgcc cccttgcgcc cgccttattg cgggcttgga tgcgggcgga tgacgggaat
     2341 ctttttcgaa ctgggtttgt tgtttgtttt gctgctggcc aatggcgtct tcgccatggc
     2401 cgagatcgcc gtggtctcat cgcgcaaggc gcggctgcag acccgcgtca acgccggcag
     2461 cagcggcgcc aggaccgcgc tggagctgct ggaggacccc ggccgcttcc tgtccaccgt
     2521 gcaggtcggc atcaccctgg tcggcacttt ggcgggcgcc tcaagcggcg cggcgctcat
     2581 cgcccggctc accccggtgc tggcccaggc gccttttctg tctgaatact gggccggaat
     2641 gctggccacc atcctcgtcg tgtctttcat caccttcctc tccgttgtca tcggcgagct
     2701 ggtgcccaag cgcctcgcca tccattcccc ggaggatgcc gccgcccgtc ttagcccgcc
     2761 gatgaacctg ctctcccgcc tggccggccc cgtggtccgg ctgctggacg cttccagcgg
     2821 cctgctggcg cgcctcctgg gcgtgcgccc cggcgaggag cccggcgtct cagaggacga
     2881 ggtccgcgcc ctcatgcacc agggcaccct gagcggagtc ttcaagccgg gggagcagcg
     2941 catggtggag ggcgtgctcg aactcgacga ccttgtcgcc gccgatgtca tgacgccgaa
     3001 aaaccagatc atctgggtgg acctcgacga gcccgacgat gaaaacgaaa agctcatggc
     3061 ggaaagcggc cacacccact ttcccgccta ccgtgacacg cgcgacaacg tgctgggcat
     3121 gatctccgtc aaagccctct gggccgcgcg gcgcggggcc tccgccggca aaacgctgga
     3181 gtccctgctc accgagccgg tctttgtctc cgagacgatg ccctgcccgc gcatcattga
     3241 ggagttccgc cgccgccgcc ggcatctggc gcttgtcgcg gacgagttcg gcggcgtgag
     3301 cggccttgtc accctgcacg atctcatgga gtccgtgcta ggctccatgc ccgacctcga
     3361 ccagcgcgcc aagccccagg tgcggcagct cgacgacggc tcctggctgg tggacgccat
     3421 gatcgccatc gaggacgcgg ccgcccgcac cggcttcacc gtcccgcagg atgagatcga
     3481 cgagggactc taccgcaccc tctccggctt cctcctgcac cgcttcggtc atgtgccgca
     3541 ggaaggcgaa aagctccagc atgacggctt cgaattcgaa atcaccgaca tggaccgcca
     3601 gcgcatggac aaagtgacga tccgtggaaa agcggcggtc cccgcgcagg cggcatgatt
     3661 ccgcgatgcc ctgcggcata aatctgatga aactctggga ggacgaagac ggtccggacg
     3721 atcgaaaatg agccaaaaat aaaatgccgc attttttttg taacaacctt tggcaaaagc
     3781 ggtatctagg gatgaggcca catgccttct tccacgtcac gcacctccca ccgtcccatg
     3841 aaacgaaaat tcatcatcca atccctcgcg gctatgcttt tggcggtcgc gatgcccatg
     3901 accgcccagg ttccacaact tattcaattc cagggccgta tcgccgttgc aggaaccaat
     3961 ttcgacggca gcggccagtt taagtttgcc ctggtgaatg ccgatggcag caccacctac
     4021 tggagcaatg acaatagcag cacggctggc gggcagcccg cagccgccgt caccctgccg
     4081 gttgtgaagg ggctttactc cgtactcctt ggggatgcga ctctgcccaa catgaccgtg
     4141 gtccccagca gcgtcttcaa caatggtgat gttcatctgc gcgtatggtt caatgacggc
     4201 gtcaatggat tccaccatct caccccggat cagcgcatcg ccgcagtcgg ctatgcgatg
     4261 atggcggcca atgtgcccga tggcagcatt acaggcgcta aaatcgcccc tggcgcggtg
     4321 ggcgcgtccc agctcgcgcc tgacgcggtg atgggcagcc ttgacgcggc tggggtcggc
     4381 ttcgtcccga gtggcgggat catcctttcc gaccaggaaa acaacacggc actcttggac
     4441 gcgggttatg tgaagatcaa cggcaccgtt cccaaacccg cctggaccga gctggcggag
     4501 tcggagattt tcaggtatga gggagagaca gtgatttgga caggcagtga agtcatctac
     4561 tggggtggta cggatttatt ttttggcgat atcaccaatt caggcgagcg gttcaacccc
     4621 tcgacaaaca cctggacacc gatgtcggat gagggagcgc catttcctcg cccctttgat
     4681 gaagtgttgt ggacgggttc ccagatgatc gtttggggtg gtgaggatat tgaccttgga
     4741 ggccccgcca acacaggcgc gatatacgat cctgaggctg acacatggac ggagatgtcc
     4801 accgaaggcg ctcctcttct catgttcccg gaggttttct gggctggtga caggatgatt
     4861 gtctggggcg gctgggatcc cattgacggc attcccacca acaccggcgc gatgtatgat
     4921 ccggaaacgg atgcttggac agaaatctcc gacgtcaacg ccccggttgc cagggattca
     4981 gcgatcattc tatggactgg cacggaaatg attgtgtggg gaggtttcga cattgatggt
     5041 ttccccgatg acaccggcgc gcgctacaat ccctccacaa acacatggac caccatgtca
     5101 tttgttggtg ccccagcttt gatgtatggt tttgaaaatc cgcctgttgt gttttggtca
     5161 ggcacagaaa tgctcatctg gggcctgtcg ggttttgatg aggatttggg attcccaatc
     5221 tacgcgggtg gccggtacaa tcccgccacc aacacctggg cggccatctc caccgaggat
     5281 gcgccggaac cttttagttt tgacgcatac gacttcaaac cccgcaccgc ctggacgggg
     5341 acggagttga tcatctggtg cggtaaagac aacgatggcg gtgcccgcta caatccggcg
     5401 acggacacct ggactccgat cagcctgaat caggcgcctg accagacgtc cggcaactcc
     5461 aggactatct ggacagggac tgaactgctg aactggggag gctggtttaa cctgagcagt
     5521 gagtacaact ctggcagccg ctacaacccg gagacggaca cctggaaaaa gatcagctcc
     5581 acagatgcgc ccttcgctta tgatccggtg ccttccgttg tctggactgg ctctcagatg
     5641 atcgtcatgc cgcatattga tccaggtttg ggagctcccg aacccgcttt cagctacaca
     5701 ccttcggaat ccgacttctt cttcatctat caacggccat gaactccagg agacttcttt
     5761 tcagcctctc cctcgcttgc gccgtcgtga ccggtctgcg cgcggaaacg cgcacgggcg
     5821 gtccctacca gctccatgcg gaccatgtgg gttccggcgg tggcaaggcg gcaggctcta
     5881 tttacatcca ggactcaacc ctgggcagca tagggggcac gtcgaatgcg gatggtatgc
     5941 tcagcaaggt gggctacatc gcccaacttt acgaggtggt gtctttggaa atcaccgccg
     6001 ctccggcgac ggtggatgaa aggggcaccc gccaactggc ttccacagcc ttgcttgatg
     6061 acggcacgct gctggcaaac ctggctggct cgacgacgtg gtcggtcctt tcagggccca
     6121 tcactggcat cagctccggt ggtctcgcct ccgcaggcac tgtgagtgaa aacacactgg
     6181 ccagcgtcca gggatcctgg tccggcttca gcgacacgct ggacctgacg gtgctggaca
     6241 tcttcgacga caggacaccc gtgtcgctgg cggcgaatct gcacaccgtc ttccagggcg
     6301 cgtccagcgt ggagatcaca ttgacccgcc cgtcgggcga ggggcctgcg agcgtcacgc
     6361 ttgccgccag caatggcacc aactccgcca tgcccccctt cacagggggc gtggttggga
     6421 aggattacca ggctctgaac acggtggtgc agtttgccgc caacgagctg gtcaaaaccc
     6481 agaccgtcac gctcattcca cggaccggca aggtgcccaa catccgattc aatgtggctc
     6541 tcaactcacc cggacccggg gccactttgg gggcgatcac ccaggcggag attcaaatcc
     6601 tggccaatga cacgaccaaa cccaccctca ctctgaagac acccgccgca ggcactgtca
     6661 gctccgtgct gccggtgctg ctcactggca ctgtcggtga cgcgctcggc attgaacggc
     6721 tggaatactc cctcaatggc tcaaccccgg aggatctgat gcttggcgct tccaccaagg
     6781 ccaccgccat ccccttctcg gcggagatcg agccccagga gggagccaac agcctgaccc
     6841 tcaccgcata cgatctgcgt ggcaacagca ccacgctcgt ccgcgacttc atcttcgagc
     6901 gccgttatgt gctcaccctc ctgcgcaacg tgcctgcggg agtggccgcc gagcctgaca
     6961 aagccggcac cctggcgctg gccgccgttc ccaccaaggc ggcatccact ctgaccaagg
     7021 gtcccgcccc gcagacatcg catgtggtgc atggatccca ggtcaccgtg acggcctcgg
     7081 cgaaaaccgg ccacattctc agccactgga ccggcctccc ggccggagcg cagaccctgg
     7141 gaaatgtggt cagctttgcc atgccagagg ccgacgtgcc cggcttgacc gccgtgtttg
     7201 tggccaatcc cttcccggct ctcgtcggca cgaaaaagcc ggtctatcag ggcctgctcg
     7261 agccagacgg catgacgcct gccaacaacg gcaccgtggg catggtcacc ggcaccctga
     7321 caccggccaa gggaaccctc agtggcaaaa tctggctgga cggcaaatcc accggcttcg
     7381 tcgcgcatct gcatggcaat ggcagcgtct ggttcctggt cgagaaaaag ctctccaccc
     7441 agttcccctt cctcggacgc tccctggaga tgtcctggtc ggatgacggg ttggacatgg
     7501 aggtcagcgg ccccgccggg acaagcgaag gtgtggcaaa aatgcccgcc tacagcaaga
     7561 cccagaccgt cggcgccgga ttgctcgacg ctctcggcaa gcagggctac tacaccgtgg
     7621 cattgcccgc caaggagcag tcccctgcca aagacaccga cacctacccg cagggcaccg
     7681 gctacaagac gctcaccctc ctggctgacg gcaccttcaa gctggccggc gtgctggcgg
     7741 acggcaccaa gatcaccgcc gccagttatc tcgtcgaggg cgacgaggcg gctgttttca
     7801 tccagctacc catccccagc acctccgcca aggatggcag cttctcaggc atgctggtct
     7861 ttgacccggc caatgacgac agcgacgtca gcgccctgga catgcgctgg taccgccccg
     7921 ccgtcgtcga aggcaccacc gtcgccaccc agctttacac cgcaggctgg cctgacggtg
     7981 tcgtgctcga tgccgtcggc gctctctacg acaagaacct cgacttccag accgtgctcg
     8041 ccctcggcgc gccggatgtg gatggcaacg cggcgctgat cttcaaagat ggcaagctcg
     8101 tcacgccgcc agacctgctc gtggtgcccg acatcaacat cacactgagc aaggtggcga
     8161 agatccccgc cacgaacaag accttcacgc tcactctgac ccaggccaag ggcgccttca
     8221 acggcacctt cacacccaac tggacccagg agaacaaaac cctgcccaag tttcaggggg
     8281 tcgtgctgca aaaaggcgcg aacaaaggcg gatacggcta cttcgtcagc aaccgcctca
     8341 ccgacctgga tccggaaagc ggcagtgtcg ctctcaaggc gtcgtccggt ctttgatcct
     8401 ggcccgcgcc actcacggcg gggatgccac acggcgtccc cgcctttttt tgccttggtg
     8461 agggtggggc aatcgtctcg attgcccatg caactgggac ggttgcacca cttggctacc
     8521 cgatctgcgc cagcacggcg gaggcgattt tgtcggcttg ggcttcgagg tattcgacgt
     8581 cgcggccctc cactagcagg cggatgaggg actcggtgcc ggagtagcgc agcagcacgc
     8641 ggccgtagtc gccgagtttt ttctcggtct ctttgatgat cttctgcgcc tcgaccagct
     8701 cggtgatggg cggcttggag cgcacgcgca ggttgcggga gctttggggg aattttttca
     8761 ggcatttgcg gagctggctg agcggctcgc cggtctcctt catgattttc aagatttgca
     8821 ggccggcgat gaggccgtcg ccggtggtgg cccagtcgcc aaagatgatg tggccgctct
     8881 gctcgccgcc gaggttcagc ccgcgctcgt gcatttgctc cagcacatag cggtcgccca
     8941 cattggtgcg gatgacgcgg ccgccgaggc gggagacgag gtcgtcgagg ccgtagttgc
     9001 tcatgatggt gacggcgagg atgttttgct tgagcgtgcc tttgcgcagc atggactcgg
     9061 cggccacggc catgagctcg tcgccatcca gggcgatggc ttcctcgtcg cacagggcga
     9121 tgcggtcggc gtcgccatcg tgggcgatcc cggcctgcgc ctggctttgg cggacgaggc
     9181 gctccagctc ctcgctgtgg gtgcagccgc actcctcatt gatgttaaag ccgtccggct
     9241 cggagtgaaa gacctgcaca tcggcgccga gacgctccag ggcctcggcg ctggtgaggg
     9301 aggcggcgcc gtgggcgttg tcgagcgcga ctctcatgcc gtccaggcgg gtgccgccca
     9361 tgctggcgac ggcgtgggcg acgtaaagtt cgacgctgtc ggccaggcgg ctgacgcggc
     9421 caatgccgcg cccctcgggc cgggccggct cggtgtcact gccgaggacg atcttctcga
     9481 tggcggactc ggtggcgtcg ttgagtttgc ggccgttgcc gtggacgaat ttgatgccgt
     9541 tgtcctcgaa ggggttgtgg gaggcgctga cgatgatgcc aaagtccgcc ccggtccgcg
     9601 cggccagcat ggccacggcg ggagtgggca gcacgccggc gagcacggca tccacgccca
     9661 cggagttcag cccggcgatg agcgcggcct ccagcatctc cccggaggcg cgcgtgtccc
     9721 gcccgatgac gcaacgcggg cgctcatgcc cctcatgccc ggcggtgagc acgcgcgcgg
     9781 cggcctggcc gagccggagc acgaactccg gcgtcatggg atggcggttg gcggtggcac
     9841 ggacgccgtc ggtgccgaag aattggcgtt tgtctgacat gggcggcgga gttagcgcag
     9901 gaggccgggg atgtcaaagc ggcggggaag cgacctcgca ttcgtgccag gccttccagc
     9961 ttgcaccaca tcgccggccg ttacttgggg agatgttcca gcaggccgcg ccctcatcgg
    10021 ggcggacaag ctcccggtgg ttgctgaaaa tcagcgctgt tcccgtgtgc ccgagttcca
    10081 gggcgagaga ggcggcattc cgccacttgg ctaggtggca ggaggcgaag ctatggcgca
    10141 tgactttgtg aggccagggc ttgagtttca agccagcggc cttttcctcc ggggtttcgc
    10201 tgccttcatc gccagagcct gccgctcgcc gtgcctcctc aaacaaggtt ctgccgcgct
    10261 gatgcttctc aggccagacg gagccagcga gctttgccac aggtgcaagc catgccgcca
    10321 gattctccca aataagaatg atccgccgcc ttgcactctt tgctttggcg gctttcacgg
    10381 tcgcgttgcc cccgcgcaga tcaacctccc gccggtcgag tttcatcaac tcggcatgtc
    10441 gaaacccggc aaagaggccc agggcgatca cagcgcggat ttcatcgtga gcaaaggcga
    10501 gcaggcgcgc tgtttctcca ggagtcagga tgccaatctc tccctcaatc tcgttcggct
    10561 tcatggaagc tgcgacaggg tttctttcgc tcaagcccat gcttaatgcc ctgctaaaca
    10621 acacacccag cacgcgccga taattgctca cggacaccgc cgagagattc agatcatgca
    10681 gccatgacga aatttcatcc gccgtcacgg aagccacctg cctgccttca aagtcccttg
    10741 cgaaccgctc caatctcaat cgcaaatcat tctggttgcg ttggctcttc ttttcgcggc
    10801 ctttgagggt cagaaggtca tccaccaact ctgccaccgt gcagggccgt tgcacgcggc
    10861 ccagatgggc aaggtagaag tccagagcct cccgcagcgt cttgccatag ggtgccagct
    10921 tttcggaagc ctccatgatc aaagcccggt ccaggtccgt gatgagtgct gccgcccgcg
    10981 tgccgatatt ggcaaccccc ttgcgggttc gagtccggcc atcggcactg agtattcggc
    11041 agcaaaacgc cggggggttc ccaccgggcg tttgcttttg gaggatgact caaagttcgg
    11101 atcacgcctt cggagtgtcc ggtccgctcg gtggcagcgc gggcttggcg atgtaatcgg
    11161 cggggatccg gcctttgagc gcgccgagga aggtgacgat gctggagacc tcctcgtcag
    11221 tcagctcctt gccgagctgg tggctggcca tctttttgac catttcctcc agcgatttga
    11281 cagagccgtc gtgcaggtag ggaccggttt cggtgatgtt gcgcaggctt ggggctttga
    11341 agaagtgctt gtcactctcc tgcttggtgg cctcgaaacg gccgaggtct ttgagttccg
    11401 gccagggttt cacgaggccg agcttttgat acatggagcc gccgaccgcc gggccgttgt
    11461 ggcaggtgac acagccggac ttggcgaagg tttcgtagcc tttgacctgc tcagccgtga
    11521 gggcgtcctt cctgcctttc agatagtcgt cccagggtgc cggggtcagc agcttgcgct
    11581 cgaaggcgcc gatggccttg ccgaagttgt cgtaggtgac cgggtcggcc tcgccgggga
    11641 aggcggcttt gaatgcctcg acatagccgg ggatggattt cagaaccttg accacgaagt
    11701 cggcgtcggg catgcccatc tcgatggggt tgagaacggg ccccttggcc tgctcctcga
    11761 ccgtcggggc gcgtccgtcc cagaattggg cgatgtgcaa agccgcattg taggtgggcg
    11821 gggagctgcg tccgcccagg ctgccgtcat gtccggggga gaagggcagg ttgtcctggc
    11881 cgtatttgtc gagctggtgg caactgttgc aggaaatctt gccgcctttg gagatgcggt
    11941 cctcataata gagcatgcgc cccaggttga tctttgcctc ggtcagctcg ttttgcggcg
    12001 agggtgcctc cgccggcagg gggcggaaca tcgcgagata agagtcggag aaggcgtcgg
    12061 cgtggacagt ggcggcggag aacgccgcca gcatgaggga atgcagggtg gtttttttca
    12121 tgggttaaat cggaccgcaa tttacgaggc gttgatgcga tccgttgcaa acttcatgcc
    12181 gcgattcttg tcattttgcg cgcggggatg gttttgacgc ggtatcgctg cccgcgccgc
    12241 tttttctttg cctcagcagc ccccggcact actctggacg catgtttgcc agattcctca
    12301 tcgccctctc ccttgccgcg gcggccatgc tcgcggccca gcagggttcc gctcctctca
    12361 gggaagtcat cgccaaactg atggaggcgc aggatttgaa aaacgccggc aaattcaccg
    12421 acgcgctgga aaagctcgac gaggtggagg cgctggcgcc ggacacgccg catctgccga
    12481 acctgcgcgg cacgatctat ctggcccccc aactgcggga cctggacaag gcggaggagc
    12541 tcttcacccg cgccattgag ttgaatccgg gcgattacat cccgaggttt catctggccg
    12601 aggtcagcta tgtgcggcat gactggcagg ccgcccgcgc cgcctttcag aagctgctgg
    12661 acgactatcc caagacaccc atggccatcc gccacatggt tgttttcaaa cggctcgttt
    12721 gcgaggtgaa gctggaccgc ttcgatgagg cggaaaagac tctcaaagag cacttcacct
    12781 tcatggatga caccgccgcc tattactacg gccacgccgc catcgccttc ggcaggcgtg
    12841 acgaggccaa ggcgaaggaa tggatcggcc gcgccgaggg catcttcaaa aaagaggaca
    12901 ggtccaccta tgaggacacc ctcatggagg cgcgctggct gccgaatatc ggcctgccgc
    12961 cactggaaaa aaggtgagcc atgtggcgga tctcggcgga cacggggggc acgttcacag
    13021 atgcctacgc gctcgacccg gaagggcggg aggcgcgctg caaggtgctc tcctcggggg
    13081 tgctgcgtgt gcgtgtggcg cgcgcggcgg gtggcgaggt ggaggtggcg ggcctaccgg
    13141 agatgccggc ggatttctgg gcggggttct cggcgcgggc agggggtgaa caacgccgcg
    13201 tgctggggat gacgggcgcg gtgctgcatc tcgacgcggc ttttgccacg gccccgatgt
    13261 tgctcgacct gacgacggag gaggaggcgc ctgtgctggc ggcgcggctg ctcacgcgta
    13321 cggcgcccgg aagtggatgt cgggcgagag cgtggcgaag atgatgcagt cgatgtcggt
    13381 ggc
//
LOCUS       DAOMYJ010000140        13316 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Prosthecobacter sp. isolate AMR_MDS_5629
            NODE_1554_length_13316_cov_21.799186, whole genome shotgun
            sequence.
ACCESSION   DAOMYJ010000140 DAOMYJ010000000
VERSION     DAOMYJ010000140.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867850
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prosthecobacter sp. (wastewater metagenome)
  ORGANISM  Prosthecobacter sp.
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Verrucomicrobiaceae; Prosthecobacter.
REFERENCE   1  (bases 1 to 13316)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13316)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.30
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:34:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,742
            CDSs (total)                      :: 4,684
            Genes (coding)                    :: 4,676
            CDSs (with protein)               :: 4,676
            Genes (RNA)                       :: 58
            tRNAs                             :: 55
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13316
                     /organism="Prosthecobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1554_length_13316_cov_21.799186"
                     /isolate="AMR_MDS_5629"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1965333"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(13..2109)
                     /locus_tag="PLP58_14305"
     CDS             complement(13..2109)
                     /locus_tag="PLP58_14305"
                     /EC_number="4.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF017076.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_function: GO:0016849 - phosphorus-oxygen lyase activity
                     [Evidence IEA];
                     GO_function: GO:0032555 - purine ribonucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HRJ09746.1"
                     /translation="MNVHHAPAGFAQRSIVLLLLGVVMAALWSMAREGAWTSAPLLQM
                     EWRVGDHVHQAGRLTETDADIVVLGIDDASLSLESAFPEDVQASRPLQLIEKGWIWPR
                     EVYAHVLDRLISAGAEKVVLDLMFATPTPGHPEYDEALRAALERHGDKVVLGADIETR
                     MVRGSPLENLVMPAQELVPQTRPFDARIGFLTYWPDADNVVRQVRAEISLTGEEADRM
                     PSLPANALGASFPAPGLGFHTPRFGSPAAYAPLSLHEVFVPDIWESNYVKPGVFAGKT
                     VFIGPTARHFQDTVMTPVGELYGVQVHAQVCAALKAGALLRECGPAPIRAAIILAALL
                     AALLVVVLKKPLLALVVLIGGGIGALALQQRAFDSWNLILPMLAPVLAWGSTGLFALA
                     WDFVLERRQKDKLRSAITRYFSPDMAEEILRKPGSYFRTLGGVQREITVLFSDLRGFT
                     SLSEKHPPDRLVRQLNQYLERMVEITFQRQGSIDKFIGDAIMAVWGRIRDGADDAALA
                     EDAVNAIATALKMRAGLDELNRQWSAEGSPPLAIGIGIHQGPAIVGNMGCAAKMEFTA
                     IGDSVNLSARLESATKQYGVDLIVSQPVAEKAGGHFRFRSADLVVVKGKKQPVQIHAV
                     LGDLSTPAPAGLGDYEEGVSLFRLGAFDKARLSFEMAAVCGLDDELTRLYLKRCDILI
                     AQPPESWDGVWVLKEK"
     gene            2272..3258
                     /locus_tag="PLP58_14310"
     CDS             2272..3258
                     /locus_tag="PLP58_14310"
                     /inference="COORDINATES: protein motif:HMM:NF021589.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HRJ09747.1"
                     /translation="MPLKPTLSWSSRLSPAWIAVPACLIGMMTAHAQTIDPSRLGEAG
                     SLPYRGTFATDEEEMQRRDLGEENGFATRSRADDDIGQQLILKETPKNRPWRAMLDEF
                     LLWSNNAANVASGEVSDWMWGQRIGIGWQQRIASHWFADLSLTQGMYRYERSNALDFQ
                     AMEAMATILDVEPRLWNVVFVGSFGFTRITNNEFQDSLLNSLSGRFGAQRLITFDRRN
                     SLQLALSGDWDLTNDVDQVFRDEYSFDLAYKYKIMRDLSLSLAYRMTYFNYRKVDRAD
                     LLHVVGATLTYTPKEWMEIYATASYALNESDISFFDYETVSGGAGLGMKVKF"
     gene            3274..5232
                     /locus_tag="PLP58_14315"
     CDS             3274..5232
                     /locus_tag="PLP58_14315"
                     /inference="COORDINATES: protein motif:HMM:NF016648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FecR domain-containing protein"
                     /protein_id="HRJ09748.1"
                     /translation="MKPLPLFLMLAVPALLEAAALKEAEVTRIINDVRLVPPRQTPAA
                     ARVGDKLRGETALATGAQSRAELRFPDQTLTRLGANSVFRLDARSRDVKLEQGVMLMQ
                     VPKQLGGATVRTAAITAAVTGTTIMMEYDPQGHVKIIALEGEVDVYLNERRSELRTLR
                     AGDMLITRPDATSLPAPVQVDLQRLRRTSKLLDAREFSALGNEKQLSEALQDQQKKKE
                     DGELLETAYVLQGRGTSITLSSEARLELLSRLPPEQRRFDFIPGTTQVDGTAQIDTRG
                     IISANNNSLSPGIVTALGAPFNADRDGSFSEFAYGIPPRFTAFDTAYEELGTWSLFKF
                     DELFIAGTPQINSSKGPRNLVFASRGDVNLRTTTLPEFPTANATGVLTLNDALDALAI
                     TTVNGDITLDPGFLISGTDQAVFLSAQGGTANVNIEGPPAGVDGASIAVPFGKLYVYA
                     GTDVVIRKAIVQADEVRLQSVRDIRLDQAEVKARSLIEMIAQNGISISNSSSLRALAE
                     TGSPSFLLEAQGPQGDIVMNGATLAGEAIRAQTLGTDGRLILGNTSINADQLVKLYAH
                     GSNGSIQFVDDVTLNSPQTILAARRVTVNNGVNVTVGQPAGLDIYTDVPQFNTPGFGD
                     FVSPGQTPITFTPRENLHPFESPQKPAF"
     gene            5398..7581
                     /locus_tag="PLP58_14320"
     CDS             5398..7581
                     /locus_tag="PLP58_14320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ09749.1"
                     /translation="MAGKQQSFFRSLLGVCVGIASIGVIAAEEPDNDAASANAAEVGK
                     AVSFTLAPLHDRDYFKVKSPGAGTLRFRVTNELRQHKSLHPWWSEESGREFRGGKWDR
                     RVAAGEVCVFAVRSSYYDFNEVTNDEVIEGVFEFTPEHSTSEPNDTAETAEVAEAGKA
                     VSFTLMPLHDRDCFKVKSPGAGTLRFRVTNELRQHKSLHPWWVEAGGNEVRGGKWDRR
                     VEAGGEYIFAVRSNYYDFNEVTNDEVIEGVFEFTPEHSPSEPNDTAETAEVVEVGKAV
                     SFTLMPRHDRDYFKVKSPGAGTLRFRVTSQLQKHQGLHVWWGEAGGNEVRGGKWDRRV
                     TSGEFCVFAVRSSYYDFNEVTNDEVIEGVFEFTPEHSPSEPNDTAETAEVVEVGKAVS
                     FTLMPRHDRDYFKVKSPGEGTLRFRVTSQLQKHQGLHLWWGEEGGNEVRSGKWDRRVV
                     AGVEYTAAVRSSYYDFNETTNDEVIEGVFEFVPDALPGEPNDTPEQAQEVEMGKPFEV
                     VFMPRHDVDFFKVTAKQDGIVRLRRLDKTETHPGLHLGWAREDGSALEGSWDHRVLAA
                     RPQAFGLRSSYHSFNESASELPVTLMAEFVPEPDAPETGDMPENARAVEPGTPFTLAL
                     TPSYDRDWFRVTAPHEGRLELRFITQPPGWVAQSWQRPGSDEPLAEEKPKVAAGDTLL
                     LGIQSRGWSSEQILQAVIQYSDGAREGPPQTRRWTFKMNRSPPAP"
     gene            7578..10985
                     /locus_tag="PLP58_14325"
     CDS             7578..10985
                     /locus_tag="PLP58_14325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ09750.1"
                     /translation="MKRTSWLIVIAAVILALWLLRQCRAPEEKTSPPPEVVFIIPEEV
                     TVGRDYNQRVLVGVKNNGPGMALLRLRSGTFPDMPSGFVGRGTDDWEVPGTVLEIPPG
                     EVWQAPLLVHANAARQDEYQVTLSALHQGVVAGEGVLTVRLAKPKLEIEATWLEVEHP
                     LDRARLRRVLRLRNIGAPVADLSLHFEREGKSADHDMHWQPVVERTRLADSESMDVMT
                     GPRLYPGFEKLAGELVIRGLNQEIRLPYQTGVPEGKSVFVTLSRTTGFASNNGNRCTN
                     QSESTYRMPPVHGTPSPSGGGGGFSGGSTGGGGRIVSATPAATPKTGEDAPKDEGKED
                     EDDDGFGFLGIDLPAGGGADKDAPEVVEESSETKDGKKKKADAKDEETEKKKEEVADA
                     TGSVPDGDFAASLLTRLNDGIMANLDSNARAGACGTLEAMLTDHANPLLPMRTLLALN
                     ETPSPSEPAPDKAGDEKKDKEKEDEKDEEKKSKSDASSDASKGSKPSRPSSVASRVDR
                     GKPRTHMVRRPLKDGAEFIDFGFGFGGTVWRRNSTRTSGPISQPRLGPSPVKDGPALA
                     AYTRPDKNGGSVAELLDPASGRVIPLSSSGKISDSPVTVLNDGGVDAIHREDGVLKRT
                     RLGEDLALNRAEGWPSGEKTGPILAAETRADGKAALLTREDAGRIMLRQPEGVREFRG
                     KAAALAFAGRESLVALQGEDGSISVLDAATGETRHTVPGTGHGAPSLITREDGGVRLS
                     YMRPVPAADPGAGGHEAGGHFAAEYKNGRWSAPTRLFTPEAPVENAAVAVEFTPPFAS
                     AHYKPMDIGFEVNGRKVAEVKGRTPSGRYLYRAPVESLNYQNGPPPDDQPPSPNAVTV
                     RSRGIGPGNFHIAQKCAMYSKHDWLQDCLVAADPAEADTLARLGTTDVRHNVVDLVLA
                     SNGTELPRSLQPGQQTPVRIGVFNAGDLPSKPARLKALGNDSEVGGADIPALAPFTGT
                     QAVVQLKAPAGWLPGAPLKLSVSIDHEGDADPSTNQLDFYLFRELDKAIAGPEAASAV
                     DLASLPPGSLTEAPAGGKDQPFESALSPARRWFRVPIPESGDLQIEVNGPDASLVDRL
                     DLFDTEGRALHPESDRWSARGSHLHLRVGLPDGAGLSADTRLRLWWE"
     gene            11007..>13316
                     /locus_tag="PLP58_14330"
     CDS             11007..>13316
                     /locus_tag="PLP58_14330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ09751.1"
                     /translation="MKYHALLLFLAAASPLAAQEPANPPAAPKAESKAEAKPVSFAGE
                     WEIHVGGRATGIVIEIAQSGGTLTGTYKEIPANNRYGIRPGFDFLNASTSGSGSMTVR
                     KGDVTKGKPVEKSQAPLKFTVEEEGRRLACVVESNEIRESERQFQLVRQQEIKSLRLF
                     TRVPGGHENAGAVIEETPMVVVIEMTGDTRRPVEVTLTSGATTLKLTASPQDVAEGED
                     GLPPGYLMTPAFLPTFGMALGVKPAPAPAEEAPPGREATEEDWKELHGNWRTLYKDGV
                     LGNVTGWAEVNEFGRLTLSYQPPGGGAPEALRLVSARVNGEVSGEPVEGAADAPPVKL
                     PKLRLEFEGRGVTSPKIEPAPKESMEKFSLDPETPEPITIKAGGFEAALPLARRGVAD
                     FRKVTLELAAIEPTGALIWDWHYKAERATQRDREGLGRVGLMLPEEKESSTAKMSGRE
                     AWMRDKPQILAAYALEDQLAVDPNITWEDRPVPKFRVGEKTRRLDSKGATDTLGISGN
                     LNRTIFIIASDLPPEENGDLRLEAAGKGGGVRQYRVLARGTEADATDAALREAWKKHR
                     AALQAAADRSAPEGVPAVAPQSDAVEKGVGVLANAILEPRATPGPQSFMLNGAEGSWQ
                     LLYGDATGRFMISRVLAESQAAGDGVAAESGELEGSEVFFVPESIRLSVEVETEIKGI
                     QEIPAVIGVQRTGVTPEFKKLVLKPDPNDALTYLSEPVELVREADYERYKDQPGALVL
                     LAKRGDVLRAQVADDSGYFLQPLTAQASIE"
BASE COUNT         2516 a         4354 c         4317 g         2129 t
ORIGIN      
        1 gttgcgcgtg cgtcattttt ccttgagcac ccagacgccg tcccaggact cgggcggctg
       61 ggcgatgagg atgtcgcagc gtttgaggta gaggcgggtg agctcgtcgt cgagaccgca
      121 gacggcggcc atctcgaagc tgaggcgggc tttgtcgaag gcgccgaggc ggaagaggga
      181 gacgccctcc tcgtagtcgc cgaggccggc gggcgcgggc gtggagaggt ctccaaggac
      241 ggcgtggatt tgcacgggct gctttttgcc tttgacgacg acgaggtcgg cgctgcggaa
      301 gcggaaatgc cccccggctt tctccgcgac gggctggctg acgatgaggt ccacgccgta
      361 ctgcttggtg gcgctttcga ggcgggcgct gaggttcacg gagtcgccaa tggcggtgaa
      421 ctccatcttg gcggcgcagc ccatgttgcc gacgatggcg gggccctggt ggatgccgat
      481 gccgatggcg agcggggggg agccttcggc ggaccattgc cggttcagct cgtcaaggcc
      541 ggcgcgcatt ttcaaggcgg tggcgatggc gttgacggcg tcctcggcga gcgcggcgtc
      601 gtcggcgccg tcgcggatgc ggccccacac ggccatgatg gcgtcgccga tgaacttgtc
      661 tatggagccc tggcgctgga aggtgatctc gaccatgcgc tcgaggtatt ggttgagctg
      721 gcgcacgagc ctgtcgggcg ggtgcttctc cgaaaggctg gtgaagccgc gcaggtcgga
      781 gaagaggacg gtgatctcgc gctgcacgcc gccgagggtg cggaagtagg agccgggctt
      841 gcgcaggatt tcctccgcca tgtccgggga gaagtagcgc gtgatggcgg agcgcagctt
      901 gtccttctgc cggcgctcca gcacaaagtc ccacgccagg gcaaagagac cggtgctgcc
      961 ccatgcgagc acgggcgcga gcatgggcag gatgagattc caggagtcga aggcgcgctg
     1021 ctgcagcgcc agcgcgccga tgccgccgcc gatgaggacg accagggcga gcaggggctt
     1081 tttcaacacc acgacgagca gcgccgccag cagcgcggcg agaatgatgg cggcgcggat
     1141 gggcgcgggg ccgcattcgc gcagcagcgc gcccgctttc agcgcggcgc agacctgggc
     1201 gtggacctgc acgccataca actcgccgac cggagtcatg acggtgtcct ggaaatgccg
     1261 cgcggtgggg ccgatgaaga cggtcttgcc ggcgaagact cccggcttga cgtaattgct
     1321 ctcccagatg tccggcacaa agacttcgtg gagggacaac ggcgcgtatg cggcggggct
     1381 tccgaagcgc ggcgtgtgga agcccagccc gggcgcgggg aaggacgcgc caagcgcgtt
     1441 cgccggcagg gagggcatgc ggtccgcctc ctctccggtg agggaaatct ccgcgcgcac
     1501 ctgccgcacc acattgtccg catccggcca gtaggtgagg aagccgatgc gcgcatcgaa
     1561 gggccgcgtc tgcgggacaa gctcctgcgc gggcatgacg aggttttcca aagggctgcc
     1621 gcggaccatg cgcgtctcga tgtccgcgcc gagcaccacc ttgtccccgt gccgctccag
     1681 ggcggcgcgc agcgcctcgt cgtattcggg atggccgggc gtgggcgtgg cgaacatcaa
     1741 gtcgagcacc actttctccg cgccagcgct gatcaggcgg tcgaggacat gcgcatagac
     1801 ctcgcgcggc cagatccagc ccttttcgat gagctgcaaa ggccgggatg cctgcacatc
     1861 ctcgggaaaa gcgctctcca ggctgaggct ggcgtcgtct atgccgagca cgacgatgtc
     1921 cgcgtcggtc tccgtcagcc gcccggcctg atggacatgg tcgccgacgc gccactccat
     1981 ctgaagcagc ggcgcggaag tccacgcccc ctcccgcgcc atgctccaaa gcgcggccat
     2041 gaccactcct agaagcagca agacgatgct gcgctgggca aagcctgcgg gcgcgtggtg
     2101 gacattcatg tggaatagct gtgggcaatc attctgccaa cacacggcat ggaaaagcct
     2161 tcacttttaa aaactttctc agtaatgagt gtagaaaccg aaaattttaa gaaagcggat
     2221 ttgattttta ttaccgattt tatcataatt acagcgaact ctcccccaaa gatgcctctc
     2281 aaacccacgc tgtcttggtc ttcccgcctt tccccggcat ggatcgcggt cccggcctgc
     2341 ctcatcggca tgatgaccgc tcatgcccag accattgacc cctcccgcct cggcgaggcc
     2401 ggcagcctgc cgtatcgcgg cacctttgcc acggacgagg aggaaatgca gcgccgggat
     2461 ttgggtgagg agaacggctt tgccacacga tccagggcgg atgacgacat cggccagcag
     2521 ctcattctca aggagactcc gaaaaaccgc ccctggcgcg ccatgctcga cgagttcctg
     2581 ctctggtcga acaacgccgc caatgtggcc tccggggaag tctccgactg gatgtggggc
     2641 cagcgcatcg gcatcggctg gcagcagcgc atcgccagcc attggttcgc ggacctcagc
     2701 ctcacgcagg gcatgtaccg ttacgagcgc tccaacgcgc tcgatttcca ggccatggag
     2761 gccatggcca ccatcctcga tgtggagccg cgcctctgga atgtggtctt cgtcggctcc
     2821 ttcggcttca cccgcatcac caacaacgaa ttccaggaca gcctgctcaa cagcctctca
     2881 ggccgctttg gcgcgcagcg cctcatcacc ttcgaccggc gcaacagcct ccagctcgcg
     2941 ctcagtggcg actgggacct gaccaacgac gtggaccagg tcttccgcga cgagtactcc
     3001 tttgacctcg cctacaaata caaaatcatg cgcgacctct ccctctccct ggcctaccgc
     3061 atgacctact tcaactaccg caaagtggac cgcgccgacc tcctccacgt cgttggcgcc
     3121 accctgacct acacaccaaa agaatggatg gaaatttacg ccaccgcgag ttatgccttg
     3181 aacgagtccg acatcagttt cttcgattac gagaccgtca gcggcggcgc gggtcttggc
     3241 atgaaagtca aattttaagc cctccctcct cacatgaaac cactcccgct tttcctcatg
     3301 ctggcggtgc ccgcgctgct cgaagccgcg gctctgaaag aggccgaggt cacccgcatc
     3361 atcaatgacg tgcgccttgt gcccccgcgc cagacccctg ccgcggcgcg ggtgggcgac
     3421 aagctgcgcg gtgagaccgc cctcgccacc ggcgcgcaat cacgcgccga gctgcgcttc
     3481 cccgaccaaa ccctcacccg tctcggcgcg aactccgtct tccgcctcga tgcgagatca
     3541 cgcgatgtga agctggagca gggcgtcatg ctgatgcagg tgcccaagca gctcggtgga
     3601 gccaccgtcc gcaccgccgc catcaccgcc gccgtcaccg gcaccaccat catgatggag
     3661 tacgacccgc agggacatgt caaaatcatc gcgctcgaag gcgaggtgga cgtctatctc
     3721 aacgagcgcc gcagcgagct gcgcaccctg cgcgcggggg acatgctcat cacccgaccc
     3781 gacgccacct ccctgcccgc gcccgtgcag gtggacctcc aacgcctgcg ccgcacctcg
     3841 aagctgctcg acgccaggga attctccgcg ctcggcaacg aaaagcagct cagcgaagcg
     3901 ctccaggacc agcagaagaa aaaggaggac ggcgagctgc tggagactgc ctatgtactc
     3961 cagggccggg gcacctccat cacgctgagc agcgaggcgc gcctggagct gctcagccgc
     4021 ctgccgccag agcagcggcg ctttgacttc atccccggca ccacgcaggt ggacggcacc
     4081 gcgcagatag acacgcgcgg catcatcagc gccaacaaca acagcctcag cccgggcatc
     4141 gtcaccgccc tcggcgcgcc cttcaacgcg gaccgcgacg gcagcttcag cgagttcgcc
     4201 tacggcatcc cgccgcgctt caccgccttt gacaccgcct acgaggagct cggcacctgg
     4261 tcccttttca agttcgacga gctcttcatc gccggcaccc cgcagatcaa ctcatccaaa
     4321 ggcccgcgca acctcgtctt cgccagccgc ggcgatgtca acctccgcac caccaccctg
     4381 cccgagttcc ccaccgccaa cgccaccggc gtcctcaccc tcaacgacgc cctcgacgcc
     4441 ctcgccatca ccaccgtcaa tggcgacatc accctcgacc ccggcttcct catcagcggc
     4501 accgaccagg ccgtcttcct ttccgcccag ggtggcaccg ccaatgtcaa catcgagggg
     4561 cctcctgcgg gcgttgatgg cgcctccatc gccgtgccat tcggcaaact ctacgtctat
     4621 gccggcaccg atgtcgtcat cagaaaagcc atcgtccagg ccgacgaggt ccggctccag
     4681 tccgtgcgtg acattcgcct cgaccaggct gaggtcaaag cccgctcgct catcgagatg
     4741 atcgcccaaa acggcatcag catcagcaac tcctccagcc tccgcgccct cgccgagacc
     4801 ggcagcccct ccttcctcct ggaggcccag ggaccgcagg gagacatcgt catgaacggc
     4861 gccaccctcg ccggcgaggc catccgcgcc cagaccctcg gcaccgacgg ccgcctcatc
     4921 ctcggcaaca ccagcatcaa cgccgaccag cttgtcaagc tgtacgccca tggcagcaac
     4981 ggctccatcc agttcgtgga cgacgtcacc ctcaacagcc cgcagactat cctcgccgcg
     5041 cgccgcgtca ctgtgaacaa tggcgtcaat gtcaccgtcg gccagcccgc cggactcgac
     5101 atctacaccg acgtgcccca gttcaacacc cccggcttcg gcgatttcgt gtcccccggc
     5161 cagaccccca tcaccttcac ccccagagaa aacctccacc ccttcgagtc cccgcaaaag
     5221 cccgcctttt gatctgagta tcgcgtccgg acgaccggac agtcatgccg ctggagtgtt
     5281 ggagtcttgg aatgttggag ctgtggcggg ccatgcacgc cattgctgca tgactccatg
     5341 acgcaggtga ctgtaatttt tctggcgcgg agcggctgct gtcattactc tgcgggcatg
     5401 gccgggaagc agcagtcttt cttccgctcc ttgttgggag tgtgcgtcgg aattgcatcc
     5461 atcggggtca tagcggctga ggagccggac aatgacgcgg ccagcgcgaa tgcggcggag
     5521 gtgggcaagg cggtgagttt cactctcgcg cctttgcatg accgggatta cttcaaagtg
     5581 aagtcgcccg gtgccggcac gctgcgtttt cgcgtcacga atgaactgcg gcagcacaag
     5641 agcctgcatc cctggtggag cgaggaaagc ggcagagaat ttcgcggagg aaagtgggac
     5701 cgcagggtgg cggccggcga agtttgcgtt ttcgcggtgc gaagcagcta ctatgacttc
     5761 aacgaggtga ccaacgacga ggtgatcgag ggcgtcttcg agttcacgcc ggagcattcg
     5821 accagcgagc cgaatgacac agcggagacg gccgaggtgg cggaggcggg caaggcggtg
     5881 agcttcactt tgatgccttt gcatgaccgg gactgcttca aagtgaagtc gcccggcgcc
     5941 ggcacgctgc gttttcgcgt cacgaacgaa ctgcggcagc acaagagcct gcatccctgg
     6001 tgggtggagg cgggcgggaa tgaagtgcgc ggcggcaaat gggaccggcg tgtggaggcc
     6061 ggcggggaat acatcttcgc cgtgcgcagc aactactacg acttcaacga ggtgaccaac
     6121 gacgaggtca tcgagggcgt cttcgagttc acgccggagc actcgcccag cgagccgaat
     6181 gacacggcgg agacggccga agtggtggag gtcggcaagg cggtaagttt caccctgatg
     6241 ccgaggcatg accgggatta cttcaaggtg aagtcaccgg gtgccgggac gctgcgtttt
     6301 cgcgtgacca gccagctgca aaagcaccag ggcctgcatg tgtggtgggg ggaggcgggc
     6361 gggaatgaag tgcgcggtgg taaatgggac cgccgggtga cgagcggcga gttctgcgtg
     6421 ttcgcggtgc gcagcagcta ctatgatttc aacgaggtga ccaacgacga agtcatcgag
     6481 ggggtcttcg agttcacgcc ggagcactcg cccagcgagc cgaatgacac ggcggagacg
     6541 gccgaagtgg tggaggtcgg caaggcggta agtttcaccc tgatgccgag gcatgaccgg
     6601 gattacttca aggtgaagtc gcccggtgaa ggcacgctgc gttttcgcgt gaccagccag
     6661 ctgcaaaaac accagggcct gcatttgtgg tggggggagg aaggcggcaa cgaggtgcgt
     6721 tccggcaaat gggaccggcg ggtggtggcc ggggtggaat acacagccgc cgtgcgcagc
     6781 agctactatg atttcaatga gaccaccaac gacgaggtca ttgagggcgt cttcgagttt
     6841 gtgcccgacg cgctgccggg cgagccgaac gacacaccgg agcaggcgca ggaggttgaa
     6901 atgggaaagc ccttcgaggt tgttttcatg ccgagacatg acgtggattt tttcaaggtg
     6961 acggcaaagc aggacggcat cgtgaggctg cggcgtctgg acaagacgga gacgcatccg
     7021 gggctgcacc tcgggtgggc gcgtgaggac ggcagcgcgc tcgaagggtc gtgggatcac
     7081 cgcgtgctgg cggcacgccc gcaggccttc gggctgcgca gcagctatca cagtttcaat
     7141 gagagcgcct cggagctgcc tgtgaccctc atggccgagt ttgtgcctga gccagacgcg
     7201 cctgagacag gcgacatgcc tgaaaatgcg cgcgcagtgg agcccggcac gccattcacc
     7261 ctggcgctga ccccatccta cgaccgcgac tggttccgcg tcaccgcgcc tcatgagggc
     7321 aggctggagt tgcgcttcat cacgcagccg cctggctggg tggcgcaaag ctggcaacgc
     7381 ccgggcagtg acgagccgct ggccgaagaa aagccaaagg tggccgccgg tgacacgctg
     7441 ctgctcggca tccagtcgcg cggctggtct tcggagcaga tcctccaggc cgtgatccaa
     7501 tattcggacg gagcgcgtga aggccctccg cagacacgcc gctggacttt taaaatgaac
     7561 cgcagcccgc ccgcaccatg aaacgaacct cctggctcat cgtcatcgcc gccgtcatcc
     7621 tggccttgtg gctgctccgc caatgccgcg cgccggagga aaaaacatca ccgccgccgg
     7681 aggtcgtctt catcattccg gaggaggtga ccgtgggtcg ggactacaac cagcgcgtgc
     7741 ttgtcggtgt gaagaacaac gggccgggca tggcgctgct gcggctgcgc tccggcactt
     7801 ttccggacat gccgagcggc ttcgtcgggc gcggcacgga cgactgggag gtgccgggca
     7861 ccgtgctgga gatcccgccc ggcgaggtct ggcaggcgcc gctgctcgtg catgccaatg
     7921 ccgcgcgtca ggacgagtat caagtcactc tcagcgcgct gcatcagggc gtggtcgctg
     7981 gagagggtgt gctgacggtg cgcctggcca aaccaaagct cgaaatcgaa gccacctggc
     8041 ttgaagtcga gcatccgctg gaccgcgccc gtctgcgccg ggtgctgcgc ctgcgcaaca
     8101 taggcgctcc agtggcggat ttgtccctgc atttcgaacg ggagggaaag agcgcggacc
     8161 atgacatgca ctggcagccg gtggtggagc ggacgcgcct tgccgattcg gagagcatgg
     8221 atgtgatgac cgggccgcgg ctttatcccg gctttgagaa gctggcggga gaactggtca
     8281 tccgcggact caaccaggaa atccgcctgc cgtatcaaac aggcgtgccg gaggggaagt
     8341 cggtcttcgt gacactctcg cgcacgacgg gatttgcctc gaacaacggc aaccgctgca
     8401 ccaaccagtc cgagagcact taccgcatgc cccctgtgca tggcactccc tcaccgtcgg
     8461 gcggaggtgg cggtttcagc ggcggttcaa ccggcggtgg tggcaggatc gtctccgcca
     8521 cgccggcagc cacgcccaag accggagagg acgcgccaaa ggatgagggc aaagaagatg
     8581 aggacgacga tggcttcggc tttctcggca ttgacctgcc cgctggcggc ggcgcggaca
     8641 aggatgcacc cgaggtggtg gaggaatcct ccgaaaccaa ggatggcaaa aagaagaagg
     8701 ctgatgccaa agatgaagaa accgagaaaa agaaagagga ggtcgcggac gcaaccggtt
     8761 cggtgccgga tggcgacttc gccgcctccc tgctcacgcg cctgaacgac ggcatcatgg
     8821 ccaacctcga ctccaacgcg cgcgcgggcg cctgcggcac gctggaggcg atgctgaccg
     8881 accacgccaa cccgctcctg ccgatgcgca ccctgctggc actgaatgag acgccttctc
     8941 cgtctgagcc agctccagac aaggctggtg atgaaaagaa agacaaggag aaggaggacg
     9001 agaaggacga ggaaaaaaaa tccaaaagcg atgccagttc cgacgcatcc aaagggtcca
     9061 agccctcgcg cccgagttcg gtggccagtc gtgtggacag gggcaagccg cgcacgcaca
     9121 tggtgcgccg tccgctcaag gatggagcgg agttcattga tttcggcttc ggttttggcg
     9181 gcacggtctg gcggcgcaac tccacgcgca cttccggccc catctcccag cctcggctcg
     9241 gcccctcgcc cgtgaaggac ggtcccgcgt tggccgccta cacgcgaccg gataaaaacg
     9301 gcggcagcgt ggccgaactg ctcgatcccg ccagcgggcg cgtcattcca ctgagttcgt
     9361 caggcaaaat atccgactca cccgtgaccg tgctcaatga cggaggcgtg gatgccatcc
     9421 accgcgagga tggcgtgttg aagcggacgc gcctgggtga ggatctggcc ctcaatcgcg
     9481 cggaaggctg gccctcaggt gagaaaaccg gccccatcct tgccgccgaa acccgcgccg
     9541 acggcaaggc ggccttgctc acgcgtgaag acgccggcag gatcatgctg cgccagccgg
     9601 aaggcgtgcg cgaatttcgc ggcaaggccg ccgcgctggc ttttgcaggc cgcgagtccc
     9661 tcgtggccct gcagggcgag gacggctcca tctccgtgct cgacgccgcc acgggcgaga
     9721 cacggcacac ggtgcccggc accggccacg gcgcgccgag cctcatcacg cgcgaggatg
     9781 gaggcgtgcg gctctcttac atgcgcccgg tgcccgccgc cgatcccggc gcgggcgggc
     9841 atgaggcggg cgggcatttc gcggcggaat acaaaaacgg ccgctggagt gctccgacgc
     9901 gtcttttcac cccggaggca ccggtggaaa atgccgccgt cgcggtcgag ttcacgccgc
     9961 cctttgccag cgcgcattac aagcccatgg atattggatt cgaggtcaac ggccgcaaag
    10021 tggccgaggt caaagggcgc acgccgtcag gtcgctatct ctaccgtgcg ccggtggagt
    10081 cgctgaatta tcaaaacggc ccgccgccgg atgaccagcc gccgagtccg aatgcggtca
    10141 cggtgcgcag ccgcggcatc ggcccgggca attttcacat cgcgcaaaag tgcgccatgt
    10201 attcgaagca cgactggctc caggactgtc tggtggccgc tgatccggcg gaggcagaca
    10261 cgctcgcgcg gctcggcacc acggatgtgc gccacaatgt ggtggacctc gttcttgcct
    10321 cgaacggcac cgagctgccg cgcagcctcc agcccggaca gcaaacgccg gtgcgcatcg
    10381 gcgtgttcaa cgccggcgac ctgcccagca aaccggcgcg tctcaaagcg ctgggcaacg
    10441 acagcgaggt cggcggcgcg gacattccgg cgctggcccc tttcaccggc acgcaggccg
    10501 tggtgcagct caaagcgccc gcaggctggc tgcccggcgc gcctttgaaa ctcagcgtca
    10561 gcattgacca tgagggtgat gccgaccctt cgaccaacca gctcgatttc tacctgttcc
    10621 gcgagctgga caaagccatc gccgggcctg aagccgccag tgcggtggac ctcgcgtccc
    10681 tcccgccggg cagcctcacc gaagcgcctg ctggcggcaa agaccagcct ttcgaatccg
    10741 cgctctcccc cgcgcgccgc tggttccgcg tgcccatccc ggagagcgga gatttgcaga
    10801 tcgaggtcaa cggccccgac gcatctcttg tggaccgcct ggacctcttc gacaccgagg
    10861 gacgcgcgct gcatcccgag agcgaccgct ggtcggcgcg cggctctcat ttgcatttgc
    10921 gggtcggtct tccagatgga gccggcttga gcgccgacac ccgcctgcgg ctctggtggg
    10981 aatgagtttg aagcccgaca catgccatga aataccacgc cctgctcctt ttcctggccg
    11041 ccgcctctcc tctggccgcg caggagcctg caaatccacc cgccgccccg aaagcggaat
    11101 ccaaagccga agcaaaaccg gtctccttcg ccggtgaatg ggaaatccac gtcggcggcc
    11161 gtgccaccgg catcgtcatc gagatcgcgc agagcggcgg cacgctcacc ggcacctaca
    11221 aggagattcc cgccaacaac cgctacggca tcaggccggg ttttgacttc ctcaacgcca
    11281 gcacctccgg ctccggcagc atgaccgtgc gcaagggcga cgtgacgaag ggcaagccgg
    11341 tggagaaaag ccaggcgccg ctgaaattca ccgtggagga ggaggggcgg cgtctcgcct
    11401 gcgtggtgga gagcaacgag atccgcgagt ccgagaggca gttccagctc gtgcgccagc
    11461 aggagatcaa aagcctgcgc ttgttcacac gcgtgcccgg cgggcatgag aacgcgggcg
    11521 cggtcatcga ggagacaccg atggtcgtcg tcatcgagat gaccggcgac acgcgccgtc
    11581 ccgtggaggt cacgctcacc tccggtgcca ccaccttgaa gctcaccgcc agcccccagg
    11641 atgtggccga gggcgaggat ggcctgcctc ccggctacct gatgacgccg gcctttctgc
    11701 ccaccttcgg catggccttg ggcgtgaagc cggcaccagc tcccgcagag gaggctccgc
    11761 cggggcgcga ggccacggag gaggattgga aggagctgca cggcaactgg cgcacgctct
    11821 acaaggacgg cgtgctgggc aatgtcaccg gctgggcgga agtgaacgaa tttggccgtc
    11881 tcacgctctc ctatcagccg cccggcggcg gcgcgccgga ggctctgcgg ctggtttcgg
    11941 cgcgtgtgaa cggcgaggtg tccggcgaac ccgtggaagg agcagccgac gctccacccg
    12001 tgaagctgcc caagctccga ctcgaattcg aagggcgcgg cgtcacctcg cccaaaatcg
    12061 aacccgcgcc gaaggagagc atggaaaaat tcagcctcga ccccgaaacg ccggagccca
    12121 tcacgatcaa ggccggtggt tttgaagccg cgctgccact ggcgcggcgt ggcgtggcgg
    12181 atttccgcaa ggtgacgctc gaactcgccg ccatcgaacc caccggcgcg ttgatttggg
    12241 actggcacta caaggccgag cgcgccaccc agcgtgaccg cgagggcctg gggcgcgtgg
    12301 gtttgatgct gccggaggaa aaagagtcct ccaccgccaa aatgtcaggg cgtgaggctt
    12361 ggatgcggga caagccgcaa atcctcgccg cctacgccct cgaagaccag ttggccgtgg
    12421 acccgaacat cacttgggaa gaccgtccgg tgccaaagtt tcgtgtgggg gagaaaacgc
    12481 gccgtctcga cagcaaaggc gccacggaca ctctgggcat cagcggcaac cttaaccgca
    12541 cgattttcat cattgccagc gacttgccgc cggaggaaaa cggagacctg aggctggagg
    12601 cggcgggaaa aggtggtggc gtgcggcaat accgtgtcct ggcgcgcgga acggaagccg
    12661 acgccaccga tgccgcgctc cgagaggcgt ggaaaaaaca ccgcgccgcc ttgcaggctg
    12721 cggcggaccg ctccgcgccg gaaggcgtgc ctgcggtcgc accccaatcc gacgcggtgg
    12781 aaaaaggcgt gggcgtgctc gcgaacgcca tcctggagcc gcgcgccact ccggggccgc
    12841 agtcattcat gctgaatgga gccgaaggaa gctggcagct tctttatggc gatgccaccg
    12901 gccgcttcat gatttcccgc gtgctcgcgg agagccaggc cgccggggac ggtgttgccg
    12961 ccgaaagcgg cgaattggaa ggctccgagg tctttttcgt gcccgagagc atccgcctca
    13021 gcgtcgaggt ggagacggag atcaaaggca tccaggaaat tcccgccgtc atcggcgtgc
    13081 agcggaccgg cgtgacgcca gagttcaaga aactcgtcct caagcccgac cccaacgacg
    13141 cgctcaccta tctttccgag ccggtcgagc tggtgcggga ggcggactac gagcgctaca
    13201 aagaccagcc tggcgcgctc gtcctgctcg cgaagcgcgg tgatgtgctg cgcgcgcaag
    13261 tcgccgatga cagcggctat ttcctccagc cgctcactgc gcaggcctcc atcgag
//
LOCUS       DAOMYJ010000141        13315 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Prosthecobacter sp. isolate AMR_MDS_5629
            NODE_1555_length_13315_cov_21.951810, whole genome shotgun
            sequence.
ACCESSION   DAOMYJ010000141 DAOMYJ010000000
VERSION     DAOMYJ010000141.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867850
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prosthecobacter sp. (wastewater metagenome)
  ORGANISM  Prosthecobacter sp.
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Verrucomicrobiaceae; Prosthecobacter.
REFERENCE   1  (bases 1 to 13315)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13315)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.30
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:34:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,742
            CDSs (total)                      :: 4,684
            Genes (coding)                    :: 4,676
            CDSs (with protein)               :: 4,676
            Genes (RNA)                       :: 58
            tRNAs                             :: 55
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13315
                     /organism="Prosthecobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1555_length_13315_cov_21.951810"
                     /isolate="AMR_MDS_5629"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1965333"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..285
                     /locus_tag="PLP58_14335"
     CDS             <1..285
                     /locus_tag="PLP58_14335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ09752.1"
                     /translation="NIAADLLFPAPVKGGGGFGLAAAAAVMPIVFAGAPIVNTITAML
                     IHPPEGGWSKLPLPFIAGCVLAAGGAFLVAKFAPSNTGTPAHSTHAQPGK"
     gene            329..1669
                     /locus_tag="PLP58_14340"
     CDS             329..1669
                     /locus_tag="PLP58_14340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009962462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CCA tRNA nucleotidyltransferase"
                     /protein_id="HRJ09753.1"
                     /translation="MLDTAVQIIQTLRAAGHEALLAGGCVRDFLLGKDPKDYDVATSA
                     TPEQVAALFPGARTVGAHFGVVIVRLGEDHVEVATFRTDGSYKDGRRPQTVTFSTPEE
                     DAKRRDFTINGIFRDPVNERVIDFVGGLEDLRAGVLRAIGDPHLRFEEDRLRLLRAVR
                     FATVLDFQIEPETWRAVRSHAAGIGDVSAERIRDELVKIFLHPHRLRGFDLLVGSGLM
                     AHVLPEILTLQGVEQPPQWHPEGDVFVHTRLMLSLLPEQVSLPLVLSVLLHDIAKPAT
                     FTVDETGRIRFNGHDKLGAEMSAEILRRLKFPNDVIEPVTTIVEHHMGFKDVKKMRKS
                     TLRRMMARPTWEDELALHRVDCLGSNGLLDNYEFMVERREEFTRQPVLPLWLVNGGDL
                     IKAGFEPGPELGKALTAVQNAQLDGVVTTREEALAWVAENTGRMPGSHDSGGDA"
     gene            complement(1671..3638)
                     /locus_tag="PLP58_14345"
     CDS             complement(1671..3638)
                     /locus_tag="PLP58_14345"
                     /EC_number="5.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009961069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016868 - intramolecular transferase
                     activity, phosphotransferases [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-sugar mutase"
                     /protein_id="HRJ09754.1"
                     /translation="MTLEAQLQAAADADLILKSTLENSLALLSASKSQVYRDSIQELV
                     DAKDWSELDDRFYQTLKFGTGGLRGRTIGMVVTKAELGSASPGQKPDHPCVGTNAMNF
                     YNVGRATRGLVSYIKKYRAEAALPGRPAIVISHDTRLFSEDFARHAARIATENGVDVH
                     LFDGCRATPEMSLAVRHLRADAGVMLTASHNPMHDNGFKVNFNDGAGIVEPHASGIIR
                     EVMSITDEEYTPLPEAEQGRLVMLGAEMDEEYLGKVQSMMLQPELLRNEKARALKIVF
                     TALHGTGGVLVPVLLRRLGFNFLTVPEQDVRDGRFPTVDSPNPENAPALQMAVDLADK
                     EGADIVIGTDPDCDRMGVGVRDAGGKMILLTGNQTGSLMCWYRLKTMFDLGILNESNR
                     KNAVILKTFVTSPMQDAIAARFGVPCVNTLTGFKWISSKLAKYEAALPEDIRAKYRDL
                     SAAESRSARLQHSKFLVFGGEESYGYMGDDFSRDKDGNGAVVMFAELAAYAASRGLTV
                     AGLLDEVYCETGYFLEVNKSKFFEGAAGAAKIAKLADSYGANPPTQVDGCVVARVRDF
                     RKPGIVDEEGEPVSTEKMLFVDLADGRSFAVRPSGTEPKIKYYMFARKAPAEGTRLSS
                     EELAAAKTSVRASLDSMWSWLEKDIESRLME"
     gene            complement(3758..5257)
                     /gene="xylB"
                     /locus_tag="PLP58_14350"
     CDS             complement(3758..5257)
                     /gene="xylB"
                     /locus_tag="PLP58_14350"
                     /EC_number="2.7.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009960013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004856 - xylulokinase activity [Evidence
                     IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0005997 - xylulose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0042732 - D-xylose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xylulokinase"
                     /protein_id="HRJ09755.1"
                     /translation="MYFLGIDSGTQSTKAIVLDLDTGGILASGHSAYDLIEGLPPGHL
                     EQHPQTWVDAVRDCVRQCLEKLGAERARIAGIGVSGQQHGLVALDADGLPVRPAKLWC
                     DTSTQAQCEEIAHHFGGQPGVIALAGNAMLPGYTIPKLLWMKQQEPENFARTASILLP
                     HDYLNFWLTGVKRMEYGDASGMGILNVRTREWCRELCDYIDPRVRSMLPPLGSSRAVH
                     GRLRPELAAEWGLSGGVIVSAGGGDNMMGAIGTGNIKPGVITASFGTSGTLYGVAGSP
                     VVDGQGEVAAFCDSTDQWLPLVCTMNVTVVTEQVRGMFGWDIRQLEAAVAGAPAGADG
                     VTFLPYLNGERTPNLPKGSGVMHGLRPANMTPAHLARAAVEGATLGLAYGLKRFRDLG
                     MNPAEIRLTGGGSKSGVWRQIAADCFNAEVVTLSTSEGAALGAAIQAAYALANEKTPA
                     VTYEELCSRLVALDETTRCRPDPARAALYAAQLERQMELTSRLNQTGWL"
     gene            5378..6295
                     /locus_tag="PLP58_14355"
     CDS             5378..6295
                     /locus_tag="PLP58_14355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007416163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HRJ09756.1"
                     /translation="MSAAIQEAPASAAPAAEKLTKKILTADHPTWCPGCGDFAVLAAF
                     YKVLEKLQYPHEKIVCVAGIGCSSRFPYFVNSHGIHFIHGRALPLATGISLSRPDVHV
                     FVFGGDGDGFSIGGNHLNHAARKNVKLTYVIMDNFVYGLTKKQTSPTSPQGFKSKTDP
                     TGSIDRPINPMKQLLASGATFIARTHAAQVKHMMDTFERAILHDGFSVVECLSECVMF
                     YAGSFDDSTPRKGGVYDLIDETQHDVKDDVAAFKLADLPSPGKFGVFYEVSRPTKNAL
                     EQKWIDSTQAKLAGTSHKEILRKRLEVMR"
     gene            6327..7004
                     /locus_tag="PLP58_14360"
     CDS             6327..7004
                     /locus_tag="PLP58_14360"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009961750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HRJ09757.1"
                     /translation="MRLLLSPLLLAAAAIAQDSPAPPPPSEYQGRVIAQTMHWQGAGW
                     LIRNKREREEGAERMREELRLKPGMTVCDLGSGNGYHTLPMAEKVGPEGRVYAVDIQP
                     EMLEMLKQRAEARMLENIECIINSETDPKIPAGSCDMILLVDVYHEFSHPEEMLAGIR
                     KALKPDGVVVLVEFRAEDESVPIKPEHKMTKAQINKELEHNGFKLAREFDGLPWQHMM
                     FFGLAEK"
     gene            complement(7011..7967)
                     /locus_tag="PLP58_14365"
     CDS             complement(7011..7967)
                     /locus_tag="PLP58_14365"
                     /inference="COORDINATES: protein motif:HMM:NF019262.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ09758.1"
                     /translation="MLSTARILLIIAAAAPVHAAVDFQKQILPVLEKKCMECHKAPFE
                     EDGRLKKPKADLRLDAAWAMLKGGETGAALVPGDLKKSYMFEVVTLPEDEDMFMPPEG
                     DPLTKDEIALLKEWIESGADFGGWTGNMEGAPKDAVAAKPATPQVREHEELYAMLEKD
                     LKPAPSDALEKARAAGAQVAPLKVDGALLRADFLTGVTKSDDEKVAALAGLKDHIARL
                     DLGRTVISDEALRTVATFTRLVALDLRQTQVTDAGLAALAKLDKLQTLNLFGTSVTDA
                     GVKHIADIKSLKQVYLFQSKVTPAGIKQLQAAIPGVKVTGGQ"
     gene            8095..9393
                     /locus_tag="PLP58_14370"
     CDS             8095..9393
                     /locus_tag="PLP58_14370"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007417314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008878 - glucose-1-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase"
                     /protein_id="HRJ09759.1"
                     /translation="MPHRIQTGAILRNSTLAIIMGGGAGTRLFPLTKDRAKPAVPLAG
                     KYRLVDIPISNCINSGVRQVFVLTQYNSASLNRHISRTYNFDQFQRGFVEVLAAQQTP
                     EGERWYQGTADAVRKNLRYFLEGDHEYYLILSGDQLYRMDFRQVMSQHLASKADITIA
                     TLPVNAQDATGFGIMKTDTEGRIHEFVEKPKDPEVLKSLEMPEATLRALSLPATEPRY
                     QASMGIYVFNRKTLVECLDNELMDFGKNIIPGALKKYHVNAYNFQGYWEDIGTIRSFF
                     NANLDLCKLVPQYDFFDSTAPIFTHARFLPATKINGARIREALISDGCIITDAHVETA
                     VVGVRAIIESGTTIRETIIMGADFYAGAAGTDPNHAPPGIGRNCRIERAIIDKNVHIG
                     DNVVITPEGKPDHMDGENFYIRDGIVVVPKGAVIPAGTWI"
     gene            9408..10265
                     /locus_tag="PLP58_14375"
     CDS             9408..10265
                     /locus_tag="PLP58_14375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009963918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HRJ09760.1"
                     /translation="MDAALPSLADFLAEPIARRALLACLMIGFANGFVSAFVVLRKSA
                     LKIGTLSHALLPGIALTVLIFGLVAWSVLAGAVIAALIVGLGSIFLSRTSRLDQDTSM
                     GILYTTAFAGGYLILTQLNVRQKLDEWLFGSIVGMADSDLWIAFGISAFAVLALTALQ
                     RPLLIHLFEPNIAASLGVPVRFLNYATFTITILVLISSLQAVGCILSVGLLVMPAATV
                     YLFTDNARTLFWGGGIIGAVGPVAAFFISYPLGWSVSATIIVVLGALFLAAYILSPRY
                     GLFSKRGRV"
     gene            complement(10355..11392)
                     /locus_tag="PLP58_14380"
     CDS             complement(10355..11392)
                     /locus_tag="PLP58_14380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009960275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HRJ09761.1"
                     /translation="MKKHNVGIIGYGWAATAHIEAINSTRQAQVTAIYSSRPLDSAEL
                     SAKHGGSIKAYQDVDAMLADPDIHVVDITSYPSQHRNQAVAAANAKKHIILEKPMANS
                     LQEVREIAAAASANGVKGCVCFECRFSGQFQSTKALIDEGVLGELHYGEVDYYHGIGP
                     WYGQFRWNIGKKDGGSALLTAGCHALDALLMVMGGEVDTVTSFSTRSKSEIFAPYEYD
                     TSSVTLVKFKNGSVGKTAAIVDCLQPYYFHTHLVGSQGSLLDNKFHSNKLKTNKAAWS
                     QLSMKMLDSGDVSDHPYATQFQAFFDALDEGRDMPLTSFTESLRSFEVIFASDRSAEQ
                     GGRPVSISEMR"
     gene            complement(11468..12826)
                     /locus_tag="PLP58_14385"
     CDS             complement(11468..12826)
                     /locus_tag="PLP58_14385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009964134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HRJ09762.1"
                     /translation="MTTPFMNRRQFSQATGSAAIITAATTLRAQQKADSQETLRIGLV
                     GCGGRGTGAAAQALGAEYNGKLVAMADVEAGQIEKSIASLSQKFPDRVDVKPDKQFVG
                     LDSYKQLIDSGVDVVLLASPPGFRPVHLMAAVDAGKHIFSEKPMAVDTIGYHLAMAAV
                     NKAREKKLSVVAGFCWRYSISRGEAFKRVLDGQIGRLASVYSTYHTGPVKPMPPASAR
                     PPGMSDVEWQVRNWYNFSWLSGDSLVEQAVHSVDKICWAMGDKPPMSCIGTGGRQIPA
                     EDGNIFDHFHAAYEWENGLVCNMASRQIKGCQGHNQDIIRGEKGVLVIGKGGVPFIDG
                     EKRWRYKGEEKNMYDLEHEALFQSIRKGEAINDGDRMMLSTLVAIMGREAAYTGQLIT
                     WDQMLACAQDLAPDDLKWGDSFTPSAMPMPGITKFLLPEPPKPEEAPASKEGEKPAQK
                     KA"
BASE COUNT         2377 a         4245 c         4179 g         2514 t
ORIGIN      
        1 aacatcgctg ccgatctcct gttcccggca ccggtcaagg gtggcggcgg attcggcctt
       61 gcggcggcgg cggcggtcat gcccatcgtt ttcgcgggcg cgccgattgt gaacacaatc
      121 acggccatgc tcatccaccc gcccgaaggc ggatggtcca aacttcccct gcccttcatc
      181 gcgggctgcg tgctggcggc gggcggcgct ttcctggtgg caaagttcgc cccttccaac
      241 acgggaacgc cggcgcacag cacgcacgct cagcccggca agtgactttt tccccggacg
      301 cgccgccgca gcgcggcgcg ctcatctgat gctcgacacc gccgttcaaa tcatccagac
      361 cctgcgcgcg gcgggtcatg aggcgctgct ggcgggcggc tgcgtgcggg acttcctgct
      421 cggcaaggat cccaaggact acgatgtggc caccagcgcc acgccggagc aggtggcggc
      481 gctttttccc ggcgcgcgaa cggtgggggc gcatttcgga gtggtcattg tccggctcgg
      541 ggaagaccac gtcgaggtgg ccaccttccg caccgacggc agttacaaag acggacgccg
      601 cccgcagacg gtgacttttt ccacgccgga ggaggacgcg aagcgcaggg acttcaccat
      661 caacggcatc ttccgcgacc ctgtgaatga gcgtgtcatt gattttgtgg gcggcctgga
      721 ggacctgcgc gcgggcgtgc tgcgcgccat cggcgatccg catctgcgct tcgaagagga
      781 ccgtctgcgc ctgctgcgcg cggtgcgttt tgccacggtg ctggatttcc aaatcgagcc
      841 ggagacatgg agggccgtgc gctcccacgc ggctggaatc ggcgatgtca gcgccgagcg
      901 catccgcgac gagctggtga aaattttcct gcatccgcat cggctgcgcg gttttgacct
      961 gctggtcggg agcggcctca tggcgcatgt gctgccggaa atcctgaccc tgcagggcgt
     1021 ggagcagccc ccgcaatggc atcccgaggg ggatgtcttt gtccacacac gcctcatgct
     1081 cagcctgctg ccggagcagg tctcgctgcc gctggtgctc tcagtcctgc tgcacgacat
     1141 cgccaaaccc gcgactttca ccgtggacga gacgggccgc atccgcttca acggccatga
     1201 caaactgggc gcggagatga gcgcggagat tttgaggagg ctgaaatttc ccaacgatgt
     1261 catcgagcct gtgaccacga ttgtggagca ccacatgggc ttcaaagacg tgaaaaagat
     1321 gcgcaagagc accctgcgcc gcatgatggc ccggcccacc tgggaggacg agctggccct
     1381 gcaccgcgtg gactgcctcg gctcgaacgg gctgctggac aattacgagt tcatggtcga
     1441 gcggcgggag gagttcaccc gccagccagt gctgccgctg tggctggtga acggtggcga
     1501 cctcatcaaa gcgggttttg aacccggccc tgagcttggc aaggcgctca ccgcggtgca
     1561 gaacgcgcag ctcgacggcg tggtgacgac gcgcgaggag gctctggcct gggtggcgga
     1621 gaacacgggc agaatgcccg gctcacatga ctcaggcggg gacgcctgag ctactccatc
     1681 aggcgggatt cgatgtcctt ttccaaccag gaccacatgg aatcgaggga cgcgcggacg
     1741 ctggttttgg cggcggcgag ctcctcgctg gaaagccggg ttccctccgc gggtgccttg
     1801 cgggcgaaca tgtagtactt gatcttcggc tcggtgccgc tgggccgcac ggcgaagctg
     1861 cgcccgtcgg cgaggtccac gaaaagcatc ttctcagtgg agaccggctc gccttcctcg
     1921 tccacaatgc cgggctttcg gaagtccctc accctggcca ccacacaacc gtccacctgg
     1981 gtcggaggat tggcgccgta gctgtcggca agtttggcga tctttgccgc gccggcggcg
     2041 ccctcgaaga acttgctttt gttcacctcc aggaagtagc cggtctcgca atagacctcg
     2101 tcgagcaatc cggcgacggt gagtccgcgg ctggcggcat aggccgccag ttcggcgaac
     2161 atgacgacgg cgccgttgcc atccttgtcg cggctgaagt catcgcccat gtagccgtag
     2221 ctctcctcgc cgccgaagac gaggaatttg ctgtgctgga gacgcgccga gcgggattcg
     2281 gcggcgctga ggtcgcggta tttggcgcgg atgtcctccg gcagcgcggc ctcatatttg
     2341 gccagcttgg agctgatcca cttgaagccg gtgagcgtgt tcacacacgg cacgccgaaa
     2401 cgcgcggcaa tggcatcctg catggggctg gtgacgaaag ttttcaaaat gacggcgttt
     2461 ttcctgttgg actcgttgag gatgccgagg tcaaacatcg tcttgagacg gtaccagcac
     2521 atgagcgagc cggtctggtt gccagtgagc aggatcattt tccctcccgc gtcccgcaca
     2581 cccacgccca tgcggtcgca gtccgggtcg gtgccgatga cgatgtccgc gccctccttg
     2641 tcagccaggt ccacggccat ttgcaaagcc ggggcatttt caggattggg cgagtccacg
     2701 gtggggaagc gcccgtcgcg cacgtcctgc tcgggcacgg tgaggaaatt gaagccgagg
     2761 cgcctcagca aaaccggaac gagcacgccg cccgtgccat gaagcgcggt gaagacaatt
     2821 ttgagcgcgc gcgccttttc attccgcaaa agctcaggct gaagcatcat ggactgcact
     2881 ttgccgagat attcctcgtc catctccgcg ccgagcatga ccagcctgcc ctgctccgcc
     2941 tccggcaggg gcgtgtactc ctcgtcggtg atggacatga cctcgcggat gatgccgctg
     3001 gcgtgcggtt cgacgatgcc agcgccgtcg ttgaaattca ccttgaagcc gttgtcgtgc
     3061 atggggttgt gcgaggcggt gagcatgacg cccgcgtcgg cgcgcagatg gcgcacggcc
     3121 aggctcatct cgggagtggc gcggcagccg tcaaaaagat gcacatccac cccgttctcg
     3181 gtggcgatgc gcgcggcgtg tctggcgaag tcctcggaga aaagccgcgt gtcgtggctg
     3241 atgacgatgg ccggcctgcc cggcagcgcg gcttcggcgc gatacttttt gatgtagctg
     3301 acgagaccgc gcgtggcgcg tccgacattg tagaagttca tggcgtttgt ccccacgcac
     3361 ggatgatccg gcttctgacc ggggctggcg gagccaagct cggccttggt gacaaccatg
     3421 ccgatggtcc gcccgcgcaa tccgcctgtg ccgaacttca aggtctgata aaagcggtcg
     3481 tccagctcgc tccagtcctt cgcgtccacc agttcctgga tgctgtcccg atagacctga
     3541 cttttgctgg cggacagcag ggccaggctg ttttccagag tggatttgag gatcaggtcc
     3601 gcgtcagcgg cggcttggag ttgagcttcg agagtcataa agagaggccg agaatagcgg
     3661 cggcgggcga cttggcaatc accgaagcgg cccggaaatc acgcgctcaa aaacgccctc
     3721 cgaaacgatg tggaagaacc tttcccgccg gactacttca aagccatccg gtctggttca
     3781 ggcggctggt cagctccatc tggcgctcga gctgcgcggc gtaaagcgcg gcgcgggcgg
     3841 ggtcgggcct gcaacgggtg gtctcgtcga gcgccaccag gcgggagcaa agctcctcat
     3901 atgtgacagc gggcgttttt tcattcgcca gcgcgtatgc ggcctggatg gcggcgccga
     3961 gcgcagcgcc ctcgctggtg ctgagtgtga cgacctcggc attgaagcag tccgcggcga
     4021 tttgacgcca gacgccgctc ttgctgccgc cgccggtgag gcgtatctcg gcggggttca
     4081 tgcccagatc gcggaaacgt ttcaaaccgt aggccaggcc cagcgtggcc ccttccaccg
     4141 ccgcgcgcgc cagatgggcg ggtgtcatgt tggcggggcg caaaccgtgc atcacgccgc
     4201 tgcctttggg cagattcggc gtgcgctcgc cgttgagata tggcagaaag gtcaccccat
     4261 ccgcgccagc gggcgcgccg gccaccgcgg cttcgagctg ccggatgtcc cagccgaaca
     4321 tcccgcgcac ctgctcggtg acgacagtga cattcatcgt gcagacaagc ggcagccact
     4381 ggtccgtgct gtcgcaaaaa gccgccacct ctccctggcc gtccaccacg ggcgagccgg
     4441 cgacgccgta aagcgtgccg ctggtgccga agctggcggt gatcacgccc ggcttgatgt
     4501 tgccagtgcc gatggcgccc atcatgttgt caccgccacc cgcgctgacg atgacgcctc
     4561 ccgaaaggcc ccactccgcc gccagctccg gacgaagccg gccatgcaca gcgcgcgagg
     4621 agcccagagg cggcagcatg gagcgcacgc gcggatcaat atagtcgcac agctcgcggc
     4681 accactcgcg ggtgcgcaca ttcaggatgc ccatgccgga ggcgtccccg tattccatgc
     4741 gcttcacgcc ggtgagccag aaattcagat aatcatgcgg cagcagaatg gatgcggtgc
     4801 gggcaaaatt ttccggctct tgctgcttca tccacagcag cttcgggatc gtgtaacccg
     4861 gcagcatggc attgcccgcc agggcgatga cacccggctg gccgccgaaa tgatgggcga
     4921 tctcctcgca ctgcgcctgg gtggaggtgt cgcaccacag cttggccggg cgcaccggca
     4981 ggccgtccgc atccagcgcc accagcccgt gctgctggcc gctgacaccg atgccggcga
     5041 tccgggcacg ctccgcgccc agcttttcca ggcactggcg cacgcagtcg cgcacggcat
     5101 ccacccaggt ttgcggatgc tgctccagat ggccgggcgg cagcccctcg atgagatcat
     5161 acgcgctgtg accggaggcc aggatgccgc cggtgtcgag gtcgagcacg atggccttgg
     5221 tgctctgggt gccgctgtca atgccgagga aatacatgat ggagtgctgt gagttggggc
     5281 ggcatcctag acgcgcccgg cgaggcgcgg caaatgaaga ttcgtgattg acccgcgcga
     5341 agaaaatcgt ttagctaggc caccaaccca ccaccccatg tccgctgcca tccaggaggc
     5401 gcccgcctcc gccgcacccg ccgccgaaaa gctcaccaag aaaatcctca ccgccgacca
     5461 tccgacctgg tgccccggat gcggtgactt cgccgttctg gccgctttct acaaggtcct
     5521 ggagaagctg cagtatccgc atgaaaaaat cgtctgcgtg gcgggcatcg gctgctcctc
     5581 gcgcttcccg tatttcgtca acagccacgg cattcacttc atccatggcc gcgcactgcc
     5641 gctggccacc ggcatttccc tgagccggcc ggatgtgcat gtgttcgtct ttggcggcga
     5701 cggcgacggc ttctccatcg gcggcaacca tctgaaccac gcggcgcgca aaaatgtgaa
     5761 gctgacgtat gtgatcatgg acaactttgt ctatggcctg accaagaagc agaccagccc
     5821 caccagcccg cagggcttca agtccaagac cgacccgacg ggcagcattg accgcccgat
     5881 caaccccatg aagcagctcc tggccagcgg agccacattc atcgcccgca cccacgccgc
     5941 gcaggtcaag cacatgatgg acaccttcga gcgcgccatt ctgcacgacg gcttctccgt
     6001 ggtggagtgc ctcagcgagt gcgtcatgtt ttacgccggc tccttcgatg acagcacgcc
     6061 gcgcaaaggc ggtgtctatg acctcataga cgaaacccag catgacgtga aggatgacgt
     6121 cgcggcattc aaactggccg acctgccctc gccgggcaaa ttcggcgtct tctacgaagt
     6181 cagccgcccg acgaaaaacg ccctggagca gaagtggata gacagcaccc aggcaaaact
     6241 ggccggcacc agccacaagg aaatcctgcg caagcggctc gaggtcatgc gctgacctga
     6301 agccgcgcgc ggcccgtcgc gatcccatgc gcctgctcct ttcccccctt ctgctcgccg
     6361 ccgccgcgat cgcgcaggac tcccctgccc cgccgccgcc ctccgagtat caaggccggg
     6421 tcatcgccca gaccatgcac tggcagggcg cgggctggct gatccgcaac aagcgcgagc
     6481 gcgaggaggg cgcggagcgc atgagggagg aattaaggct caaacccggc atgaccgtct
     6541 gcgacctggg cagcggcaac ggctatcaca ccctgccaat ggcggaaaaa gtggggcctg
     6601 aaggcagggt ctatgccgtg gacatccagc ccgagatgct ggaaatgctc aagcagcgcg
     6661 cggaagcgcg gatgctggag aacatcgaat gcatcatcaa ctcggagacg gatccgaaaa
     6721 tacccgccgg ctcgtgcgac atgattctgc tcgtggatgt gtaccacgag ttctcccatc
     6781 cagaggaaat gctggcgggc atccgcaagg cgctgaagcc ggacggcgtg gtggtgctgg
     6841 tggagttccg cgccgaggac gagagcgtgc cgatcaaacc ggagcacaaa atgaccaagg
     6901 cgcagatcaa caaggagctg gagcacaacg gcttcaaact cgcgcgcgag tttgacgggc
     6961 tgccctggca gcacatgatg ttttttggcc tcgctgaaaa gtgatgccgc tcactgcccg
     7021 cccgtgactt tgacgccggg gatggctgcc tggagttgct tgatgcccgc gggtgtgacc
     7081 ttgctctgga agagataaac ttgcttgagc gatttgatgt ccgcgatgtg cttgacgccc
     7141 gcgtcggtga cgctcgtgcc gaacaggttg agtgtctgca gcttgtcgag cttcgccagc
     7201 gcggcgagac cggcgtcggt gacctgtgtc tggcgcaggt ccagcgccac aaggcgggtg
     7261 aaagtggcga ctgtccgcaa cgcctcatcg ctgatgacgg tgcgccccag gtccagccgg
     7321 gcaatgtgat ccttcagtcc ggccagcgcg gcgacttttt catcgtcgct cttggtgaca
     7381 ccggtgagaa agtcagcccg cagcagcgcg ccatccactt tcaacggggc gacctgcgcg
     7441 cctgcggccc tggctttttc cagggcgtcg gaaggcgcgg gtttgaggtc cttttcgagc
     7501 atggcataca gttcctcgtg ctcgcgcact tgcggggttg cgggcttcgc ggccaccgcg
     7561 tccttgggcg cgccttccat gttccccgtc cagccgccga agtccgcccc ggactcgatc
     7621 cactccttca gcagggcgat ctcgtccttg gtcagcgggt cgccttccgg cggcatgaac
     7681 atgtcctcat cctcaggcag ggtgacgact tcaaacatgt agcttttctt caaatcacca
     7741 ggcacgagcg ccgccccggt ctcgcctccc ttgagcatgg cccatgcggc atcaaggcgc
     7801 aggtcggcct tcggcttctt gagccgcccg tcctcctcaa agggcgcctt gtggcattcc
     7861 atgcactttt tttccagcac gggcaggatt tgtttctgaa aatccacggc ggcatgaact
     7921 ggagcggcgg cggcaatgat gagcaagata cgggctgtcg aaagcatggc ttggggaggg
     7981 ttttgccttt cagtgggtca tgtcggtttt acccgcctga aaaacaggcg agagaaacgc
     8041 ttgcctccag gtcttgtttc agggaaaact cagccacaac ccgtctccat gtccatgccc
     8101 caccgaatcc aaaccggcgc catcctgcgg aactccacac tcgcgatcat catgggaggc
     8161 ggcgccggca caaggctctt ccccctgacc aaggaccgcg ccaagccggc cgtgccgctt
     8221 gcggggaaat accggctggt ggacatcccg atcagcaact gcatcaactc aggcgtgcgc
     8281 caggtctttg tgctcaccca gtacaacagc gcctcgctca accggcacat ctcccgcacc
     8341 tacaacttcg accagttcca gcgcggcttc gtggaggtgc tggcggcgca gcagactccc
     8401 gagggcgagc gctggtacca gggcaccgcc gacgcggtgc gcaaaaatct gcgctacttt
     8461 ctcgaaggcg accatgagta ctatctcatc ctcagcgggg accagcttta ccgcatggac
     8521 ttccgccagg tcatgagcca gcatctggca tccaaggcgg acatcaccat cgccaccctg
     8581 cctgtcaatg cccaggacgc cacaggcttc ggcatcatga agactgacac cgagggacga
     8641 atccatgaat tcgtggaaaa acccaaggat cccgaagtgc tcaagagcct tgagatgcct
     8701 gaggcgacac tgcgcgcgct gagcctgccc gccacggagc cgcgctacca ggcatccatg
     8761 ggcatttacg tcttcaaccg caaaactctg gtcgagtgcc tcgacaacga gttgatggac
     8821 tttggcaaaa acatcatccc cggcgcgctc aagaagtatc atgtgaacgc ttacaacttc
     8881 cagggctact gggaggacat cggcaccata cgctcgtttt tcaacgccaa cctggacctc
     8941 tgcaagctgg tgccccagta cgatttcttc gactccacag ctcccatatt cacgcatgcc
     9001 cgcttccttc cagccaccaa gatcaacggc gcccggattc gcgaggcgtt gatctccgac
     9061 ggctgcatca tcaccgacgc ccatgtggag accgccgtcg tcggcgtgcg cgcgatcatt
     9121 gagagcggca cgaccattcg tgaaaccatc atcatggggg cggatttcta cgcgggagcc
     9181 gccggcaccg atccgaacca cgcgcctcca ggcatcgggc gcaattgccg cattgagcgc
     9241 gccatcattg ataaaaacgt ccacatcggc gacaacgtgg tcatcacccc cgaaggcaag
     9301 ccggaccaca tggacggaga gaatttctac attcgtgacg gcattgtcgt ggtccccaaa
     9361 ggcgctgtga tacccgccgg cacctggatt tgagctttcc acgctgaatg gatgccgcgc
     9421 tgcccagcct tgccgatttt ctggccgaac ccatcgcccg ccgggcgctt ctggcgtgtt
     9481 tgatgatcgg attcgccaac ggtttcgtca gcgccttcgt ggtgttgaga aagtcggcct
     9541 tgaagatcgg cacgctatcc cacgctctgc tgccgggcat cgccttgacg gtcctcatct
     9601 ttggcctggt ggcctggagc gtgctggcgg gcgcggtcat cgccgcgctc atcgtcgggc
     9661 tgggctccat cttcctctcg cgcacctcgc gcctggacca ggacacctcg atgggcatcc
     9721 tctacaccac cgcctttgcc ggcggctacc tgatcctcac ccagctcaat gtgcgccaga
     9781 agctggatga atggctcttt ggcagcatcg tgggcatggc cgacagcgat ttatggatcg
     9841 ccttcggcat cagcgccttc gccgtcctgg ctctcaccgc gctgcaaagg ccgctgctca
     9901 tccacctctt cgagccgaac atcgccgcca gcctcggcgt gcccgtgcgc tttctgaatt
     9961 acgccacttt caccatcacc atcctcgtcc tgatctcctc gctccaggcc gtgggctgca
    10021 ttctcagtgt cggtcttctg gtcatgcccg cggcgacggt ttacctcttc accgacaacg
    10081 cgcgcacgct gttctggggc ggcgggatca tcggagccgt cggtccggtg gccgcttttt
    10141 tcatcagcta tcccctgggc tggagcgtca gcgccaccat catcgtcgtg cttggcgcct
    10201 tgttcctggc cgcctacatc ctcagtccgc gctacgggct tttcagcaaa cgcgggcgcg
    10261 tctgaggatg ggacgctgac actggcaccc caaaaaaagc caggtgtgcc tgcctcgcat
    10321 ggctgcgcca tacgggcgtg ccgggtgtgc gggatcaccg catctcgctg atgctcacgg
    10381 gcctgcctcc ctgctcggcg ctgcggtcgg aggcgaagat gacctcgaag ctgcgcaggg
    10441 actcggtgaa gctggtcagc ggcatgtccc tgccctcgtc gagcgcgtcg aagaaggcct
    10501 ggaactgggt ggcgtagggg tggtcgctca catcgccgga gtcgagcatc ttcatggaga
    10561 gctggctcca agcggccttg ttggtcttga gcttgttgct gtggaatttg ttgtcgagca
    10621 gactgccctg gctgccgacc agatgggtgt ggaagtagta gggctgaagg cagtccacaa
    10681 tggcggccgt cttgccaacg gagccgtttt tgaacttcac gagcgtgacg ctggaggtgt
    10741 catactcgta aggcgcgaag atttcgcttt ttgagcgtgt gctgaagctg gtgacggtgt
    10801 ccacctctcc gcccatgacc atgagaaggg cgtcgagcgc gtggcagccg gcggtgagca
    10861 gggcgctgcc gccgtccttt ttgccgatgt tccagcggaa ctggccgtac caggggccga
    10921 tgccgtggta gtagtccacc tcgccgtagt gcagctcgcc gaggacgccc tcgtcaatga
    10981 gcgccttggt ggactggaac tggccggaga agcggcactc gaagcagacg cagcctttga
    11041 cgccattggc agaggccgcg gcggcgatct cgcgcacctc ctggagcgag ttcgccatcg
    11101 gcttctccaa aatgatgtgc ttcttcgcgt tggcggcggc cactgcctgg ttgcgatgct
    11161 ggctggggta gctggtgatg tccaccacat ggatgtcagg gtcggcgagc atggcgtcca
    11221 cgtcttgata cgccttgatg ctgccgccgt gcttggcgct gagctcggcg ctgtcgagcg
    11281 ggcgggacga gtagatggcg gtgacctgcg cctgccgggt ggagttgatc gcctcaatgt
    11341 gggcggtggc cgcccaaccg taaccgatga tgcctacgtt gtgttttttc atgatgaatc
    11401 aaagtgtgct ggcggcggac gcgggccgcc gggttctcat tcattccagg acagttcagc
    11461 gcgcttgtca cgcctttttc tgcgcgggct tttcaccttc cttggatgcc ggggcttctt
    11521 ccggcttggg cggctccggc aggaggaatt tcgtgatgcc cggcatgggc atggccgagg
    11581 gggtgaaact gtcgccccac ttcaaatcat ccggcgccag atcctgcgcg caggcgagca
    11641 tttggtccca ggtgatgagc tggccggtgt aggcggcctc gcggcccatg atggcgacga
    11701 gggtggagag catcatgcgg tctccgtcgt tgatggcctc ccccttgcgg atggactgga
    11761 agagcgcctc gtgctcaagg tcgtacatgt tcttttcctc gcctttgtag cgccagcgct
    11821 tttcaccatc aatgaaggga acgccgccct tgccgatgac aagcacgccc ttctcgccgc
    11881 ggatgatgtc ctgattgtgg ccctggcagc ctttgatctg gcgggaggcc atgttgcaga
    11941 cgagaccgtt ttcccactca taagcggcat ggaagtggtc gaagatgttg ccgtcctcgg
    12001 cggggatctg ccgcccgccg gtgccgatgc agctcatggg cggcttgtcg cccatggccc
    12061 agcagatttt gtccacgctg tggacggcct gctccaccag gctgtcaccg ctgagccagg
    12121 agaagttgta ccagttgcgc acctgccact ccacatcact catgcccggc gggcgcgcgc
    12181 tggcgggagg catgggtttg accggcccgg tgtggtaggt ggaatagacg ctggccagcc
    12241 tgccgatctg gccatccagc acgcgtttga aagcctctcc gcgtgaaatg ctgtagcgcc
    12301 agcagaaacc ggccacgaca ctaagcttct tttccctcgc cttgttcacc gccgccatcg
    12361 ccaggtggta gccaatggtg tcaacggcca tgggtttttc gctgaaaatg tgctttcccg
    12421 catccaccgc agccatcaaa tgcaccggac gaaatcccgg agggctggcc agcagcacca
    12481 cgtccacgcc gctgtcaatg agctgcttgt aggaatccag accgacaaac tgcttgtccg
    12541 gcttcacatc cacgcggtcg gggaatttct gcgagaggct ggcgatgctc ttttcgatct
    12601 gaccggcctc cacgtcggcc atcgccacca gtttcccgtt gtactcggcg cccagagcct
    12661 gcgccgccgc acccgtgccg cgcccgccgc agcccacgag gccgatgcgc agcgtctcct
    12721 gggagtcggc tttctgctgc gccctcaggg tcgtggcggc ggtgatgatg gcggctgagc
    12781 ccgtggcttg cgaaaactgg cggcggttca tgaagggtgt ggtcatgcga ggcaagaaat
    12841 cgcggaaggc gcggaagacc tttcagagga aaaccatctg tccgcgaacc cggacgcatg
    12901 aacttgaagt gcgcaagagc acatgaccgc cccccaagac gtttcctgca gttcggacat
    12961 gaattcgaga gggcggcgag ggcttcggtg acatcaagga atgagccggt gtccataagg
    13021 cccaaaaaac ctgcggcccg gaagcgaatg atgagtgtcg taagccgtct tcagccatga
    13081 aagcggaacg gtgaaaaaag ttgcacaatg acaccatttc ctggctcgcc ttcgtgcgcg
    13141 gagaaagatg cctgcggcgc atcatctcac ggaagcacat gcttttgcca cggaagctgt
    13201 ggctttttcc acggaaacac gcgcatcggc ggcggcggcg cgggcccctg cggggggctt
    13261 gtgcgatgcg tgagggttgc cagccagttc atggagccac tctccggcaa accgg
//
LOCUS       DAOMYJ010000142        13300 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Prosthecobacter sp. isolate AMR_MDS_5629
            NODE_1557_length_13300_cov_27.828237, whole genome shotgun
            sequence.
ACCESSION   DAOMYJ010000142 DAOMYJ010000000
VERSION     DAOMYJ010000142.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867850
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prosthecobacter sp. (wastewater metagenome)
  ORGANISM  Prosthecobacter sp.
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Verrucomicrobiaceae; Prosthecobacter.
REFERENCE   1  (bases 1 to 13300)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13300)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.30
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:34:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,742
            CDSs (total)                      :: 4,684
            Genes (coding)                    :: 4,676
            CDSs (with protein)               :: 4,676
            Genes (RNA)                       :: 58
            tRNAs                             :: 55
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13300
                     /organism="Prosthecobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1557_length_13300_cov_27.828237"
                     /isolate="AMR_MDS_5629"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1965333"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(369..896)
                     /locus_tag="PLP58_14390"
     CDS             complement(369..896)
                     /locus_tag="PLP58_14390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ09763.1"
                     /translation="MKHVILIPVFYALTCPLLAGESALTKTYQPLDGLGAGSIVISQV
                     TCHEWYSHSGQPTSIGLISAANVPPTNNPKEAIENLNLASVCGVRFSCGDMDLTSELT
                     LDATAFAIPERFDHPREDIVRASLECLRRCLPDKLRQTPVTLKSSNENEAWLAEIVRQ
                     FNMHDRSKVFFTPAQ"
     gene            complement(908..3208)
                     /locus_tag="PLP58_14395"
     CDS             complement(908..3208)
                     /locus_tag="PLP58_14395"
                     /inference="COORDINATES: protein motif:HMM:NF019259.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1592 domain-containing protein"
                     /protein_id="HRJ09764.1"
                     /translation="MPRAFFSHHCEKCHSGEKPKGKFDITKLGTDFSDASNREHWQRV
                     LEQIQSGDMPPEDKPRPPEQDANTAMKWIRGQVDAIELTRRAKEGRVVLRRLNRAEYA
                     NTMRDLLGVEVDLADLLPPDTSTNGFDNNAELLHTSSHLLRNYLDAADRVLDEAIASK
                     PKPWILNKRFDIKDERTVKPNGSVYRHVPDGVAIFAAWESANIRVTMWNFRSHVRGRY
                     RFRISAYAIQNEGKPVTYRVTAGTLKEVTEERLVGYFSVPQDKPTVIEFTEQLEPENR
                     IRILAEGLPATPPQVQQVGAENYKGPGLVVQWVDIEGPLLESWPPPSHRALFGDLKQE
                     LVERERYEVVSAQPEADAERLLTDFARRAFRRPVSSREIQPFLARVRSALENGRSFEQ
                     AMRLGFKGILVSPDFLFLRERGPRLNDFELASRLSYFLWSSMPDEELLKLAAANQLHE
                     PEVLRGQLDRLLRDPKSRSFTENFTGQWLKLRAIDDTLPDRTLYPEYDDILKTAMLKE
                     TKLFFDEVLSQDLPLTNFVHSDFTFLNERLAKHYRIPGVEGMDMRKVTLPAGSHRGGL
                     LTMGSILKVTANGTTTSPIIRGSWVLERILGTPPPKPPPDVEAIEPDIRGATTIREQL
                     AKHRNVESCASCHKKIDPPGFALESFDVIGGWRTHYRATGEPRIIDGRRRRYWDGPAV
                     DPGDVTPDGRRFENINDYKQLLIENKEQLARNLAEKLLAYGTGAAPTSTDDTLIEKII
                     SRVRARNFGFKSLIHEVVQSDLFQTK"
     gene            complement(3288..4142)
                     /locus_tag="PLP58_14400"
     CDS             complement(3288..4142)
                     /locus_tag="PLP58_14400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ09765.1"
                     /translation="MLLVEKFEEGTLEALKPRWNEIENKDAQVLEIVNDHPAASSGSK
                     CLQVTATLGENTGGHLFKRLDPIQTRVFSRFYVKFAEDAPYLHHFSGLGGYNPPTNWP
                     QGSAGKHAAGDTRFSARIEPNGSYGTLAAPGGWMFYTYWHDMKPSADGGFWGNGLWPE
                     GKPGPPRGQWQCVEIMVKCNDIGQSNGELALWLDGKLTTHIGPGTKTGKWTGEGFDVL
                     KDGPQTFEGFRWRTVPELAVSYFMLSHYVTENAYRQNNVANPPKTNRVWFDDIVIATE
                     YVGPISKR"
     gene            complement(4349..4594)
                     /locus_tag="PLP58_14405"
                     /pseudo
     CDS             complement(4349..4594)
                     /locus_tag="PLP58_14405"
                     /inference="COORDINATES: protein motif:HMM:NF021041.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="pectate lyase"
     gene            complement(4815..6626)
                     /locus_tag="PLP58_14410"
     CDS             complement(4815..6626)
                     /locus_tag="PLP58_14410"
                     /inference="COORDINATES: protein motif:HMM:NF012471.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M14 family metallopeptidase"
                     /protein_id="HRJ09766.1"
                     /translation="MKRQALALPILALACVCLSAFTPDEFLPPAPPWSGASEALIVPQ
                     GHPWITPCEQSGMTDSPPYAETVAWLEKLAAASPLVRVITFGRTAQGRPLCAVIASRE
                     GISEAAKLPANGKPTVLAQGGIHSGEIDGKDAGMMLLRDIAFGGKGTLLDHANLLFIP
                     VLNADGHERRSIWNRPNQRGPIHQGWRTTAQNLNLNRDYMKAQAPEMAAVLKLIQESK
                     PALYLDIHVTDGVDYQYDITYGFQGWDGGFAWSPKIGQWLDQTLRPALDADLKTAGHI
                     PGPLVFAKNKRDLSQGITLKPYDPRFSTGYGDLRHLPTLLIENHSLKPFRQRVLGTCI
                     LIESCLRIVAQHRDDLQQRISEDEAARPQKISLNWNRAADHQTTMNFAGIASDNSNSP
                     ASGTQEVRWLGTPQDHPKLPVHHLTKPGLTVTRPKAYFIPVTKPDILSVLASHGIRME
                     PLKEDTTLTLDHYRLMDPVVSATPFEGRHTMTTQVKTETGKHTLPAGTMRVSTDQPLG
                     DLAIALLEPEHLDSLAAWGFVPEILQRTEYIEGYVIAPLAERMMENDPKLKAEFEARL
                     ATDPAFAKDPEARLQWFYERSPFYDLEYLRYPIAIER"
     gene            complement(6631..9561)
                     /locus_tag="PLP58_14415"
     CDS             complement(6631..9561)
                     /locus_tag="PLP58_14415"
                     /inference="COORDINATES: protein motif:HMM:NF013123.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HRJ09767.1"
                     /translation="MKAPLTAIALILFFCQLPAQSQGPEIILNQASADIFPQGWRSGR
                     VNASAQPLLDTEVERLRGIVKRALGKYPPALLETTLKKVHGLGHLEYHGVATGGTRSA
                     SAIYVVCKPHYSDAAVERIIHAEYSSVLFQKFAQNFDAGAWQRQNAEGSTYLGSGVAA
                     VKAGKVSGQATPELQEQGFISEYAKASIEEDFNSHVARIFMGDETYWQTLEKHPRLKA
                     KTGLLIAFYQKLDPSMNPAKFDAIRKEASKLTLDRIFTDKEFEEEKMETFQWSKLGAH
                     YFILEKPAEGKEGKDLVRHDAATGARTVIIPAREFTPDGEKKPLTVSDFAFSMDEQRL
                     LIFTDTKKVWRKNTRGDYWLLDLATKKLAKLGGDAAPSTLMFAKFSPDGSRVAFVQKN
                     NLYMQNLADMKITALTADDSGNLINGTSDWVNEEELGLRDCFRWSPDGTRILFWQFDT
                     SEVKRFTLVNQTDGSYPRLTTFPYPKAGEKNSATRLGVVPASGGAVTWLKVPGDPREH
                     YLPSAEWTPDGGRILMQQFNRLQNSLLVMLADPASGEVAPLFTETDPAWVENRNSEPR
                     WLGGEFLWVSERSGWRRVLRVDLTGQTLPVTPDGQDVIEVTALDAKGGWLYYLASPDN
                     ATQRHLHRASLDGMEEQRITPAGQPGTHGYSCSEDAAWAVHTRSSMTSPPVVDLVSLP
                     KHETVRKLKTQSALKEKLANMRLPKTEFLRVDIGDGITLDGWMMSAADPGSKSKHPLL
                     MHVYGEPAGQTVKDSWGGQKTLWHWMLAQQGYIVASVDTRGTPAPRGRDWRKSVYRQL
                     GIINAREEAAAVRMLLQRHDYLDPQRVGIWGWSGGGSSSLDAIFRYPDLYHTAIAVAP
                     VPDRRLYDTIYEERYMGLPQDNEEGYKDGSPITHAANLQGNLLIVHGTGDDNVHYQGV
                     EKLMDALIANNKSFTVMPYPNRDHSINTGKGTTRHLHELMTRFLNQHLPVTKSAPPAP
                     DPKP"
     gene            complement(9600..12869)
                     /locus_tag="PLP58_14420"
     CDS             complement(9600..12869)
                     /locus_tag="PLP58_14420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009961087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional proline dehydrogenase/L-glutamate
                     gamma-semialdehyde dehydrogenase"
                     /protein_id="HRJ09768.1"
                     /translation="MSPSQPVIRHASTSHPGTDGEAKTAKQAIQLAEQLLKRALGQQS
                     AGEKREAERLGRLMNDPQGKAFTFAMVDEVFRSHDASASAKRWRRLVALHGLPQYLPL
                     ADRALMGIGAAGSLILPNVVMKAVAARLRADSARVILPREREPLHRYLASRKEAGFRV
                     NLNHLGEAVLGEEEARRRFEAVLAHLADPAVEYISVKISAIFSQIHLIAWDETLHAIQ
                     DRLRPLYRAAQKDGKFVNLDMEEYRDLALTLAAFRGLLDEPEFQQLRAGIALQAYLPD
                     SFSAMQELTQWARRRVAAGGAPVKIRLVKGANLAMEKVEAEMHGWHPAPYATKAETDA
                     NFRRLLDHGCRPENAMAVRLGVASHNLFDVALALTLREQNGVRDHVEIEMLEGMANHQ
                     ARAVRDAAGAMLLYAPAVRSHDFLSAMAYLVRRLDENTAPENFLRDMFAMRPGSPEWE
                     NQKRRFIQGWRDRHIVSADSRRSRPLELPASGFHNAPDTDFTQPSARAALQEAIHTWQ
                     PVPLPALPDLETTLRTACSAQNDWELLGVAGRAAIIHRAADIMEQQRFAALACLRADG
                     KKAAGDADGEVSEAIDFARYYARTAGAPAGVAAEAMGVIAVVSPWNFPYAIPAGGVLA
                     ALMAGNSVILKPARATGQTAWLLINQLWQAGVPREVLHFFPCDGQAGRKMLTDERVAA
                     CILTGGYETARKFQSWRPSLPLYAETSGKNALVITAQADRELAIKDLVRSAFGHSGQK
                     CSAASLAILEAEVYDDPNFRRQLRDAAASLHAGPATDPRSIVTPLVNPPGRDLCRALT
                     TLDAGEEWLLEPKQLSDDPCLWSPGIKLGVKPGSWFHLTECFGPVLGVMRADSLAQAT
                     EWQNATDFGLTAGIHSLDPDEISWWRDRVRAGNLYINRPITGAIVRRQPFGGWKKSCI
                     GPGAKAGGPNYVMSFCRLREANGATPDYAQAWQNHFAKDHDPSALACESNIFRYRPCR
                     GVILRLDTPDEHFIKLARLASETTGAPLALSIRSEESDTAFIARLPALAKQAEFLRTL
                     SAPGDEVLHAAFEAGLNWINAPILCNGRVELARWLREQSVSQTLHRYGQITAQNGAPR
                     S"
     gene            13014..>13300
                     /locus_tag="PLP58_14425"
     CDS             13014..>13300
                     /locus_tag="PLP58_14425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009961086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HRJ09769.1"
                     /translation="MKTTQARKLSRRDAIFAQAPALEAAEFLFDTVGDVLFCVKDRER
                     RYVAANEAFIRRAGLVHRSDLLGRTAREVFPPILAAGYEQQDDEVFHRGATV"
BASE COUNT         2445 a         3768 c         4431 g         2656 t
ORIGIN      
        1 aaaagcccag cggcgcgcag atgcagatca tgcgccgcgg tgtcggcgaa gcgctcgcgg
       61 catgaccgac ggaaagcttg tccagccacg ggagagccag cgagatgccc gtggtgcgga
      121 ggaaatggcg gcgggtgttc atgagacaat gagacaatga gacaatgaga caatgagaca
      181 atgagacaat gagacaatga gacaatgaga caatgagaca atgagacaat gagacaatga
      241 gacaatgaga caatgagaca atgagacaat gagacaatga gacaatgaga caatgagaca
      301 atgagacaat gagacaatga gacaatgaga caatgagact gggagaagag gagatatgga
      361 gagctggttt attgagctgg cgtgaagaag actttgctcc ggtcatgcat gttaaactga
      421 cggacgatct cagctaacca agcttcgttc tcattcgatg actttagagt gacaggagtt
      481 tgacgcagtt tgtctggaag gcagcgcctc aggcattcca gggaagcccg aacgatatct
      541 tcacgcggat ggtcgaatcg ctccgggatt gcaaacgcgg tggcatccaa agttaactcg
      601 gaggtcagat ccatatctcc acagctaaac cggactccac aaactgaggc gaggttcaga
      661 ttttctatcg cttcttttgg gttgttggtc ggcgggacgt tggctgcaga aatcaagcca
      721 atggaagtgg gctgaccgct gtgtgaatac cattcatgac aagtgacctg agaaatgaca
      781 atcgaacccg caccgagtcc atccagcggc tgataagtct tagttagcgc agactcacct
      841 gccaataggg gacaagtcaa tgcgtagaag actgggataa ggatgacatg tttcatgttt
      901 gtagaagtca cttcgtctga aagagatcgc tctgcacgac ctcgtggatc aacgacttga
      961 agccaaagtt cctggcgcgg acgcgagaga taattttctc gatcagggtg tcatcggtgg
     1021 acgtgggggc ggcaccggtg ccgtaggcga ggagtttttc ggcgaggttg cgggcgagtt
     1081 gctctttgtt ctcgatgagc agttgcttgt agtcattgat gttttcaaag cggcggccgt
     1141 cgggtgtgac atcaccggga tcgacggcgg ggccatccca gtagcgacgg cggcggccat
     1201 caatgatgcg tggctcgccg gtggcgcggt agtgcgtgcg ccagccgccg atgacatcaa
     1261 agctctccag cgcgaagccg ggtggatcga tcttcttgtg gcagcttgcg cagctttcga
     1321 cgttgcgatg cttggcaagc tgctcgcgga tggtggtggc accgcggatg tccggctcga
     1381 tggcttcgac atccggcggc ggtttcggcg gcggtgtgcc gaggatgcgc tccagcaccc
     1441 aggagccgcg gatgatgggc gaggtggtcg tgccattcgc tgtgaccttt aaaatgctgc
     1501 ccatggtgag caggccgccg cgatgactgc ccgcaggcag cgtgactttg cgcatgtcca
     1561 tgccctccac gccggggatg cggtaatgct tcgcgagccg ttcattgaga aaagtgaagt
     1621 cagagtggac gaagtttgtc agcggcaggt cctggctcag cacttcatca aagaagagct
     1681 tggtctcctt cagcatcgcg gttttgagga tgtcgtcata ctccgggtag agcgtgcgat
     1741 ccggcagcgt gtcatcaatg gcgcggagtt tgagccactg accggtgaag ttctccgtga
     1801 aggatctcga tttcggatca cgcagcaggc gatcgagctg gccgcgcaac acctcgggct
     1861 cgtgcagttg gttggcggcg gccagtttta aaagctcctc gtccggcatc gagctccaca
     1921 ggaagtaaga gagccgactc gccagctcga aatcattgag gcgcggtccg cgttcgcgca
     1981 ggaagaggaa gtcgggtgaa accaagatcc ctttgaagcc aaggcgcatg gcctgctcaa
     2041 agctgcgtcc gttttccaag gcactgcgca cacgcgcgag gaagggctgg atctcccgcg
     2101 aggacacggg ccgacgaaaa gcgcgacgag cgaaatccgt gagcaagcgc tctgcatccg
     2161 cctcgggttg tgcggagacg acctcgtagc gctcgcgctc cacaagttcc tgtttgagat
     2221 cgccgaacaa ggcgcggtga ctcggcggcg gccaggattc gagcagcggg ccttcgatgt
     2281 ccacccactg caccaccagg ccggggcctt tgtaattctc cgctcccacc tgctgcacct
     2341 gcggcggcgt ggcgggcaga ccttccgcga gaatgcggat gcggttctcc ggttccaact
     2401 gctcggtgaa ctcgatcacc gtcggcttgt cctgcggcac ggaaaaatac ccgaccaacc
     2461 gctcctcggt cacctctttc agcgtgccgg cggtgacgcg gtaggtgacg ggcttgccct
     2521 cgttttgaat ggcataggcg gagatgcgga aacgatacct gccgcgcaca tggctgcgga
     2581 agttccacat cgtcacgcgg atgttcgccg actcccacgc cgcaaaaatg gccacgccgt
     2641 ccggcacatg gcgatacacg ctgccgttcg gcttcacggt gcgctcgtcc ttgatgtcga
     2701 agcgcttgtt cagaatccac ggcttcggtt tgctggcgat ggcctcgtcg agcacgcggt
     2761 cggcggcgtc gagatagttg cgcagcagat gagaggaggt gtgcagcagc tccgcgttgt
     2821 tgtcgaagcc attggtcgag gtgtcaggcg gcagcaggtc ggccaaatcc acttccacgc
     2881 cgagcaaatc gcgcatcgtg ttcgcatact cggcacgatt cagccgccgc agcaccacac
     2941 gaccttcctt ggcgcgacga gtcagttcaa tcgcgtccac ctgaccacgg atccacttca
     3001 tggcggtgtt ggcgtcctgc tccggtggac gcggcttgtc ctcgggtggc atgtcaccgc
     3061 tttggatctg ctcgagcacg cgctgccagt gttcgcgatt gctggcatcg ctgaaatccg
     3121 tgcccagctt cgtgatgtcg aacttgccct tcggtttctc gcctgagtgg catttctcgc
     3181 agtgatgaga gaagaaggcg cgaggcatct gcccagcagc ttgcacagct ccaaagagga
     3241 tcagtgccag aacagccgtg ctgaagcgcg aagaagacat ggacgtatca acgcttcgaa
     3301 atcggcccga catactcggt ggcgatcacg atgtcatcga accatacacg attggtcttg
     3361 ggtggattgg cgacgttgtt ttggcggtag gcgttttcgg tgacgtagtg gctgagcatg
     3421 aagtagctga cggcgagctc ggggacggtg cgccagcgga agccttcaaa ggtctgcggg
     3481 ccgtccttga gcacgtcgaa gccttcgccg gtccattttc cggttttggt gccggggccg
     3541 atgtgcgtgg tgagcttgcc atcgagccac agggcgagct cgccgttgct ctggccgatg
     3601 tcgttgcatt tcaccatgat ctcgacgcac tgccactggc cgcgaggcgg gccgggtttg
     3661 ccttcgggcc aaaggccgtt gccccagaag ccgccgtcgg cgcttggctt catgtcgtgc
     3721 cagtaggtgt aaaacatcca gccgccgggc gcggcgagcg tgccgtagct gccattcggc
     3781 tcgatgcggg cgctgaagcg tgtgtcgccc gcggcgtgct tgccggcgct gccttgcggc
     3841 cagttggtgg gcggattgta gcctccgagg ccgctgaagt gatgcagata aggcgcgtct
     3901 tcggcgaact tcacatagaa gcgcgaaaag acacgggtct ggatgggatc gaggcgcttg
     3961 aaaagatgcc ccccggtatt ctcgccgagc gtggcggtga cttggaggca tttggagccc
     4021 gaactggccg ccggatggtc gttcacgatt tcgagcactt gggcgtcctt gttctcgatt
     4081 tcgttccaac gcggcttcag ggcctccagc gtgccttctt caaatttttc tacgaggagc
     4141 acggcgggat gaatcttgat atcagtgtcg ccggggtgga accgggagat gccaaagcct
     4201 tccggcagcg gctcacgcgc gcttgccaga gtggaaatca aacacgcaga cagagagacg
     4261 cggcacaggg ggatcatgcc acctttacgt ctgcgtgatc ccaaggcttg cagtcgccca
     4321 gcccagacca ttcgcagcat ggtgagactc agtgattcgt ggcaagcccg tttttccgca
     4381 gcacatgagg tgtgtcactg ctgggcgtgc tggatgcgcc acttggggta atccgctgca
     4441 gcaggctggc ggcccaggtg ccatgatagg cataaccgtt gcggcgctcg cagccaatct
     4501 tattgaagtc gtaacggaag aaggagtcca agtctaggca tggcgcgcgg ttcgtttcca
     4561 gctcataaaa gctcgcccgc agaagtgggc ttgagggtct atgaagattt gcctgcggat
     4621 gcgcccggcg cgcgaactgc gcgaagacac cgactgagca tcatcgagat cagccagcgc
     4681 atcggttgag acgggtgcaa atactgcatc cacgtcctca agaaacacac agggaccgcg
     4741 cccccgaaat atcggaaagt gaaacgggct tgatgattga ctgttgctac aagaccaaag
     4801 ggtgcggata aatatcaccg ctcaatcgct attggatacc gcaggtattc caggtcatag
     4861 aacgggctgc gctcatagaa ccattgcagg cgggcttcag ggtctttcgc gaaggcgggg
     4921 tcggtggcga gcctggcctc gaactcggct ttgagtttcg ggtcgttctc catcatgcgc
     4981 tcggcgagcg gggcgatgac gtagccttcg atgtattcgg tgcgctggag gatttcgggc
     5041 acgaaacccc aggcggcgag ggagtcgagg tgctccggct ccagcaaagc gatggcgaga
     5101 tcgcccagcg gctggtcggt ggacacgcgc atcgttccgg caggcagggt gtgcttgccg
     5161 gtttcggtct tgacctgggt ggtcatggtg tggcggcctt caaagggcgt ggcggagacc
     5221 accgggtcca tcaggcggta atgatcgagc gtgagggtgg tgtcttcctt gagcggctcc
     5281 attcggatgc cgtgcgaggc cagcacgctg aggatgtcag gcttggtgac ggggatgaag
     5341 taggccttcg ggcgggtgac ggtgaggcct ggtttggtaa ggtgatgcac agggagcttg
     5401 gggtgatcct gcggggtgcc aagccagcgc acttcctgcg tgccggaggc gggcgagttg
     5461 gaattgtcgc tggcgatgcc ggcgaagttc atcgtggtct ggtggtcggc ggcacggttc
     5521 cagttgaggc tgattttttg cggccgcgcg gcttcgtctt cggagatgcg ttgttggagg
     5581 tcatcgcgat gttgggccac gatgcgcagg caggactcga tgaggatgca ggtgccaagc
     5641 acacgctggc ggaagggctt caaagagtga ttctcgatga gcagcgtggg cagatgccgc
     5701 aggtcgccgt agccggtgga gaaacgcggg tcgtagggct tcaaggtgat gccctgtgaa
     5761 aggtcgcgtt tgtttttggc aaagacgagc ggtccgggga tgtggcccgc cgtcttcaag
     5821 tcggcgtcca atgctgggcg cagcgtttga tccagccact ggccgatttt tggcgaccag
     5881 gcgaagcctc catcccagcc ttggaagccg taggtgatgt cgtattgata gtccacgccg
     5941 tcggtgacgt ggatgtcgag atagagcgcg ggtttggatt cctggatgag cttcaagacg
     6001 gcggccatct ccggcgcctg agccttcatg tagtcgcggt tgaggttgag gttctgcgcc
     6061 gtggtgcgcc agccttgatg gatggggccg cgctggttgg ggcggttcca gatggagcgg
     6121 cgctcgtggc cgtcggcgtt cagcacggga atgaaaagca ggttggcatg atcaagcagc
     6181 gtgcccttgc cgccgaaggc gatgtcgcgc agcagcatca tgcccgcgtc cttgccgtca
     6241 atctccccgg aatgaatgcc accttgcgcg agcacggtgg gcttgccgtt ggcggggagt
     6301 ttggccgcct cggagatgcc ttcgcgactg gcgatgacgg cgcaaagcgg acggccttgc
     6361 gcggtgcggc cgaaggtgat cacgcgcacc agcggcgagg cggcggccag tttttccagc
     6421 caggcgacag tctccgcgta aggcggtgaa tcagtcatgc cgctttgctc gcatggcgtg
     6481 atccagggat ggccctgcgg cacgatcagg gcctcgctgg cacccgacca tggtggcgcg
     6541 ggcggcagga actcgtccgg cgtgaaggcg gacaggcaga cacaggcgag cgcgaggatg
     6601 ggcagtgcga gggcttgtcg tttcatgtgc tcagggtttg ggatcgggcg caggcggcgc
     6661 gctttttgtc acaggcagat gctggttcag aaaacgcgtc atcaactcgt ggagatgacg
     6721 cgtggtgccc ttgccggtgt tgatggaatg gtcgcggttc gggtagggca tgacggtgaa
     6781 ggacttgttg ttcgcgataa gcgcgtccat cagcttttcc acgccctggt aatgcacgtt
     6841 gtcgtccccc gtgccatgaa cgatcaagag gttgccttgc aaattcgcgg cgtgggtgat
     6901 gggcgagccg tccttgtagc cctcctcgtt gtcctgcggc aggcccatgt agcgctcctc
     6961 gtaaatcgtg tcatagaggc ggcggtccgg cacgggggcg acggcaatcg cggtgtggta
     7021 aagatcggga taacgaaaaa tggcgtccag gctgctgctg ccgcccccgc tccagcccca
     7081 gatgccgaca cgttgtggat cgagataatc atggcgctgc aaaagcatcc tcaccgcggc
     7141 ggcctcctcc cgcgcattga taatgccaag ctggcggtaa acggacttcc tccagtcccg
     7201 tccgcgcggc gcgggcgtgc cgcgcgtgtc cacgctggcg acgatgtagc cctgctgagc
     7261 gagcatccag tgccagagcg tcttctggcc gccccacgaa tctttgacag tctggcccgc
     7321 gggctcgcca tagacatgca tgagcagagg gtgctttgac ttggagccgg gatccgccgc
     7381 gctcatcatc cagccgtcca gagtgatgcc gtccccgatg tccacgcgta gaaactcggt
     7441 ctttggcagc ctcatgttgg ccagcttttc cttcaatgcg gactgtgtct tcagcttgcg
     7501 cacggtctca tgtttcggca gcgagacgag gtcaacgacg ggaggcgagg tcatgctgga
     7561 gcgggtatgc acggcccagg cggcatcctc agagcatgag tagccgtgcg tccccggctg
     7621 gccggcgggt gtgatccgct gctcctccat gccatccagg ctggcgcggt gcaggtggcg
     7681 ttgcgtggca ttgtccggcg aggccagata ataaagccag ccgcctttgg catcgagggc
     7741 tgtcacttcg atcacatcct ggccgtcagg tgtcaccggc agcgtttggc cggtcaaatc
     7801 cactcgcaac acccgacgcc agccgctgcg ctcgctcacc cacaggaatt cgccgcccag
     7861 ccagcgcggt tcgctgttcc tgttctcgac ccaggctggg tccgtttcgg tgaacagtgg
     7921 agccacctcg ccggatgccg gatcagcgag catgacgagc aggctgtttt gcaggcggtt
     7981 gaactgctgc atgagaatcc tgcccccgtc cggcgtccac tcggcggaag gcaggtagtg
     8041 ctcgcgcggg tcgcctggaa ccttcagcca ggtcaccgcg ccgccgctgg caggcaccac
     8101 tccaaggcgt gtggccgagt ttttttcacc ggctttgggg taaggaaagg tggtgaggcg
     8161 cgggtagctg ccatccgtct ggttcacgag cgtgaagcgc ttcacctcac tcgtgtcgaa
     8221 ctgccagaat aagattcgtg tgccgtcagg actccagcgg aaacaatccc gcagccccag
     8281 ttcctcctcg ttcacccagt ctgaggtgcc gttgatcaga ttgccggagt cgtcggcagt
     8341 cagcgcggtg attttcatgt ccgccaggtt ctgcatgtac aggttgtttt tttgcacaaa
     8401 ggccacgcgt gagccgtcgg gcgagaactt ggcaaacatc aaggtcgagg gcgcggcatc
     8461 accgccgagc ttggcgagct tctttgtcgc gagatcgagt agccagtagt caccgcgcgt
     8521 atttttgcgc cagacctttt tcgtgtcggt gaaaatcagg agccgctgct cgtccattga
     8581 gaaggcgaag tctgaaactg tgagcggctt cttttcccca tccggcgtga actcgcgcgc
     8641 tgggatgatg accgtgcgcg ctccagtggc tgcgtcgtgc ctcaccaaat ccttgccttc
     8701 ctttccctca gccggctttt ccaggatgaa gtaatgcgcg ccgagcttgc tccattgaaa
     8761 agtctccatc ttctcctcct cgaactcctt gtcggtgaaa atgcggtcca gcgtgagctt
     8821 cgacgcctcc ttgcggatcg catcaaactt tgccgggttc atgctcgggt cgagcttctg
     8881 gtaaaaggct atcagcaggc ccgtcttggc tttgaggcga ggatgcttct ccaatgtctg
     8941 ccagtaagtc tcatcgccca tgaaaatgcg ggcgacgtgc gagttaaaat cctcctctat
     9001 gctcgccttg gcatactcgc tgataaaacc ttgttcttgg agttctggcg ttgcctggcc
     9061 agagaccttg cccgccttca ccgccgccac accactgccc agataagtgg aaccctccgc
     9121 attctgccgc tgccaagccc cggcatcgaa gttttgcgcg aacttttgaa acagcacact
     9181 ggagtattcc gcatggatga ttcgctccac cgccgcgtca ctgtaatggg gcttgcagac
     9241 cacatagatg gcgctggccg agcgcgtgcc gcctgtggcc acaccgtgat actccagatg
     9301 ccccaggcca tgcaccttct tcagcgtcgt ttccagcagg gctggtgggt acttgcctaa
     9361 cgcgcgcttc acgatgccgc gcagccgttc cacctccgtg tccagaagag gttgcgcgct
     9421 ggcgttgacg cgtccgctcc gccagccttg ggggaagatg tccgccgagg cttgattgag
     9481 aatgatctcc ggcccttgag actgagccgg caactggcag aagaataaaa tgagagcgat
     9541 cgcagtcagg ggggctttca tggcgggata ggcaacgagg ggacggtaat gaatgcgaat
     9601 caagagcggg gcgcgccgtt ttgggcggtg atctgcccgt agcgatgcag ggtctggctc
     9661 acgctttgct cgcgcagcca gcgggcgagc tcgacgcgcc cgttgcagag gatgggagcg
     9721 ttgatccagt tcaaacctgc ctcaaacgcg gcatgaagca cctcgtcccc cggtgcggac
     9781 agcgtgcgga gaaactcggc ttgctttgcc aaggcgggca gcctggcgat gaaagcggtg
     9841 tcgctttcct cggagcgaat gctgagcgca agcggcgcgc ccgtggtctc ggaggccagg
     9901 cgggccagct tgatgaagtg ctcgtcgggt gtgtcgagcc gcaggatgac gccgcgacag
     9961 gggcggtagc ggaagatgtt cgattcacat gccagcgcgc tcgggtcgtg atccttggcg
    10021 aagtggtttt gccatgcctg cgcatagtca ggagtcgcgc cgttcgcctc acgcagacgg
    10081 cagaagctca tcacatagtt cggcccgccg gctttggctc cggggccgat gcaggacttc
    10141 ttccagccgc cgaagggctg gcgccgcacg atggcgccgg tgatggggcg gttgatgtag
    10201 aggttgcctg cgcggacacg atcacgccac catgagatct catcgggatc gagcgagtga
    10261 atgcccgcgg tgagtccgaa gtcggtggcg ttctgccatt ccgtggcctg cgcgagcgag
    10321 tccgcgcgca tgactccgag cacggggccg aagcattccg tgaggtggaa ccaggagccg
    10381 ggtttgacac cgagcttgat gccgggagac cagaggcagg gatcgtcgct gagttgtttc
    10441 ggctccagca gccattcctc accggcatcg agtgtggtga gcgcgcggca caggtctctg
    10501 ccgggtgggt tgacaagagg ggtgacgatg ctgcgtggat cggtggctgg accggcatgg
    10561 aggctggcgg cggcatcgcg aagctggcgg cggaagttgg ggtcgtcata gacctccgct
    10621 tcgagaatgg cgaggctggc ggcgctgcat ttctggccgc tgtgaccgaa cgcggaacgc
    10681 acgaggtctt tgatggctag ctcgcgatcc gcctgcgcgg tgatgacgag cgcgtttttg
    10741 ccactggtct cggcataaag cggcagggag ggacgccagc tttggaactt gcgcgcggtc
    10801 tcatagccgc cggtgaggat gcaggccgcg acgcgttcat cggtgagcat tttccggccg
    10861 gcctggccgt cacaggggaa gaaatgcagc acctcgcgcg gcacgccggc ttgccagagt
    10921 tggttgatca ggagccaggc ggtctgcccg gtggcacggg cgggtttgag gatgacactg
    10981 tttcccgcca tcaaggcggc aagcactccg ccggccggaa tcgcataagg gaagttccag
    11041 ggagaaacga cagcgatgac gcccatggct tccgctgcaa cgcctgcggg cgccccggca
    11101 gtgcgggcgt agtagcgtgc gaagtcaatg gcctcagaga cttcgccatc cgcgtctccc
    11161 gccgcttttt tcccgtccgc acgcaggcag gccagggcgg cgaagcgttg ctgctccatg
    11221 atatcggcgg cgcggtggat gatggcggcg cggccggcga cgccgaggag ttcccagtca
    11281 ttctgggcag agcaggcggt gcgcagggtg gtttcgagat cgggcagggc agggaggggg
    11341 actggctgcc aggtgtgaat ggcttcttga agagcggcgc gggcggatgg ctgggtgaag
    11401 tcagtatcgg gggcgttgtg gaaacctgat gcgggcagtt ccagagggcg gctgcggcgt
    11461 gagtctgctg agacgatgtg gcggtcgcgc cagccttgga tgaagcgccg tttctggttc
    11521 tcccactccg gtgagccggg gcgcatggcg aacatgtcgc gcaggaaatt ttccggcgcg
    11581 gtgttttcat ccagacggcg gaccagatag gccatggcgc tgaggaaatc atggctccgc
    11641 accgcgggcg cgtacaggag catggcgccg gcggcatcgc ggacggcgcg ggcctggtgg
    11701 ttggccatgc cttccagcat ctcgatctcc acatggtccc gcacgccatt ttgctcgcgc
    11761 agtgtgaggg cgagggcgac atcgaaaaga ttgtggctgg cgacgccaag gcgcacggcc
    11821 atcgcgttct ccggccggca gccgtggtcg agcaggcggc ggaagttggc atcggtctcc
    11881 gccttggtgg catacggcgc ggggtgccag ccgtgcatct cggcctcgac cttttccatc
    11941 gcgaggttcg cgcctttgac gaggcggatc ttgaccggcg cgccaccggc ggcgacgcgc
    12001 cgcctggccc attgagtcag ctcctgcatg gccgaaaacg agtccggcag ataagcctgg
    12061 agggcgatgc ccgcgcgaag ctgctgaaac tccggctcat ccagcagacc gcgaaaggcg
    12121 gcgagggtca gcgcgaggtc acgatactcc tccatgtcga gattcacgaa cttgccgtct
    12181 ttttgcgcgg cgcggtaaag cgggcgcagc ctgtcctgaa tggcgtgcag cgtctcatcc
    12241 caggcgatga gatggatctg gctgaaaatg gcggagattt tcacggagat gtactccacc
    12301 gccgggtccg cgagatgcgc gagcacggcc tcgaaacggc gccgcgcctc ctcctcgccc
    12361 agcacggcct cgccgagatg gttgaggttg acgcgaaatc ccgcttcttt gcgcgaggca
    12421 aggtagcggt gcagcggctc cctttctctg ggcaggatga cacgcgcact gtccgccctc
    12481 aggcgcgccg ccacggcctt catgacgaca ttcggcagga tcaggctgcc tgccgcgcca
    12541 atgcccatga gcgcccggtc cgccagcggc agatactgtg gaaggccatg cagtgccacc
    12601 agccttctcc atcgtttggc ggaggcactg gcgtcatggc tgcgaaaaac ctcgtccacc
    12661 atggcaaaag tgaaggcttt gccttgcgga tcattcatga gcctgccgag tctctccgcc
    12721 tcgcgctttt caccggcaga ctgctgccca agcgccctct tcaacaactg ctccgccagt
    12781 tggatggctt gtttggccgt ctttgcctcc ccatcggtgc cgggatggga agtcgaagca
    12841 tggcggatca cgggctggct gggagacatg gccccatgat gccggaaaag cgcgcccagg
    12901 tcgtgtcatt tttcgcttcg gcgcatgacg aaaaaagcca aagccgcagg ctgtgctgga
    12961 gctttcagcc ggcttcctgc aagatggggc tgttgccaaa cccgcttcgc gccatgaaga
    13021 ccacccaagc ccgcaagctg tcccgacggg acgccatctt tgcccaggca cccgcgctgg
    13081 aggccgccga gtttctgttc gacacagttg gggatgtgct tttttgcgtg aaggaccgcg
    13141 agcgccgcta tgtggcggcg aacgaggcat ttatacgtcg tgccgggctg gtgcatcgga
    13201 gcgatctcct ggggcgcacg gcgcgcgagg ttttcccccc cattctggcc gctggctatg
    13261 agcagcagga tgacgaggtg ttccacaggg gagcgaccgt
//
LOCUS       DAOMYJ010000143        13163 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Prosthecobacter sp. isolate AMR_MDS_5629
            NODE_1578_length_13163_cov_21.374428, whole genome shotgun
            sequence.
ACCESSION   DAOMYJ010000143 DAOMYJ010000000
VERSION     DAOMYJ010000143.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867850
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prosthecobacter sp. (wastewater metagenome)
  ORGANISM  Prosthecobacter sp.
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Verrucomicrobiaceae; Prosthecobacter.
REFERENCE   1  (bases 1 to 13163)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13163)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.30
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:34:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,742
            CDSs (total)                      :: 4,684
            Genes (coding)                    :: 4,676
            CDSs (with protein)               :: 4,676
            Genes (RNA)                       :: 58
            tRNAs                             :: 55
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13163
                     /organism="Prosthecobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1578_length_13163_cov_21.374428"
                     /isolate="AMR_MDS_5629"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1965333"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..654
                     /locus_tag="PLP58_14430"
     CDS             <1..654
                     /locus_tag="PLP58_14430"
                     /inference="COORDINATES: protein motif:HMM:NF019349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FCD domain-containing protein"
                     /protein_id="HRJ09770.1"
                     /translation="TVCGKLAALARHDLQNEDGWLPTERELSTQLGVSRSVVREAAKR
                     LELQGLIEIRQGSGMKFVDKLHKPLSSALNLLVPDEKRRLVHLTEIRQALEPENARLA
                     ATRATASQKRALRQTQNLLEQTLDFEEQIQADMDFHRLLAEASGNPIAALLMHSLSDL
                     MQASLAHGYRRVTPAQAIKQHSGILQAIEKRDPAAAARAMQRHLLSAFADLGLEHSH"
     gene            662..2062
                     /locus_tag="PLP58_14435"
     CDS             662..2062
                     /locus_tag="PLP58_14435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009964656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1501 domain-containing protein"
                     /protein_id="HRJ09771.1"
                     /translation="MNASLEFSRRQFLGQMTTGLTGVALSRLFSAESKTQPHFTPKAK
                     QVLQIFCPGAASHVDLWDHKPMLEKYDGTPLPGAAEVTFQGKNGNLMKSPWSFRAAGQ
                     SGKRISSMLTHMARHVDDIAFIHSMSSRTNTHGPGCINMNSCFTREGFPSAGSWVSYA
                     LGSLNENLPTYIAIQDLRGEPPNGKANWSNGFLPAQYQAVAMAAQQPLRNLARPAAIS
                     SDEEKATRDFLQFINSQHAAAHPGNSELQARMAAYEMAAKMQLTAPEVSDLSGEPQHV
                     HDLYGTGDPNKLKAAYAKNCLLARRFLEQGVRYVSLYCASRASAVDGLLNWDAHKTLK
                     ADYERHCPIFDQPTAALLTDLKQRGMLNDVLVVWCTEFGRMPTHQQGTSGRDHNPDAF
                     TTWMMGAGIKGGISHGETDEFGRRAAVDVATVYDFYATVLHLLGLDHEKLTYYHNGAK
                     RRLTDVHGHVLTPLLA"
     gene            2091..3911
                     /locus_tag="PLP58_14440"
     CDS             2091..3911
                     /locus_tag="PLP58_14440"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arylsulfatase"
                     /protein_id="HRJ09772.1"
                     /translation="MTHKLHFILFAFLAVVCGQSAGEKPNILLIITDDQGYGDLSIHG
                     NTHLATPHLDKLAGTGVQFERFYVNSFCAPTRAALLTGRWPLRTGCHGVTHNREAVRP
                     SETTLAEALRPAGYRSACIGKWHNGEQHPFTPQGQGFDEFFGFNNGHWNDYFDAVLLR
                     GTRHEATQGYIADVLTDEAMKFISANKSGPFFCYLSYNTPHSPYQVPDKYFDKFKAKG
                     LENTLAAFYGMCENIDDNVGRLLAHLEAEHLAKNTIVLFLTDNGGTAGVKTYNAGMRG
                     GKTSVHEGGSRVPLFIRWPAANWTPHVVKPITSHTDLYPTLMDLCGVQAPSGPPVDGT
                     SLRPLLEKPDAAGWPERTLFTHNPINETNKYPGAVRTQKYRLVREITGPAGGSKAKAN
                     DASAKPWQLYDMENDPGQTNDIASEHPEIVKDLAARYDAWFADISKEGLQRYPLPVGH
                     LEHNPVELHAPQAYFDPPLQFASGPGFANDWLTAWTDAETKVWWEIEASEAGDYELEL
                     AYGASPADAGSRIRLYAGNSAIEVTLPAAEAKEIPLPHRDEAGKARYRNRDWATLKAG
                     TLNLPKGPAKITLEPLSKPGAQVMDLKHLQMKLQPKAPEK"
     gene            3908..5533
                     /locus_tag="PLP58_14445"
     CDS             3908..5533
                     /locus_tag="PLP58_14445"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HRJ09773.1"
                     /translation="MKALPFLVAFFAILGGQSSAAKPNVLFILADDLGWGDLRCYGNP
                     RIDTPVLDSLAQEGARLTAHYSPSPLCSPARAGFLTGRYNHRTGAVDVPSNRGLDRLD
                     LSEKTFGDYFRHAGCATALIGKWHNGLYCRDYLPHQRGFDLFHGFPNGGQDYWKWNLL
                     RNDDAVPHDGRYLSDALNDETLKFITAHKEKPFAIFLAHHAPHSPLQAPEPLVEKYRQ
                     RLGKDASEAVAVTYAMIEAMDTGLGRVFQTLKDNGQWENTIIVFTSDNGPVLTKDPKL
                     GPQQRYNGGFSGEKQHVLEGGIRVPCIVAWPGRIPAGQVIATPVHGCDWLPTLFSLTQ
                     TDAPAGAKPFDGQNIMPLLMGQPAPALAGRTLLFQRNRYAPVAHCNAAIRQGRWKLYW
                     PGDDSSLKKDSARDNPSYQRGITHPHWEMPLDRQLDAPTSAPQPAPRLYDLDADPAEQ
                     KNLASEHPEIVRALAQKHDAWFAEIIPEWRQSRARLLEHDRAYWEDRPTPDPAAIFDG
                     FWLWKSAPQPVNPKTADPLKVFRGYWNTSQNDE"
     gene            5584..8433
                     /locus_tag="PLP58_14450"
     CDS             5584..8433
                     /locus_tag="PLP58_14450"
                     /inference="COORDINATES: protein motif:HMM:NF019223.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1553 domain-containing protein"
                     /protein_id="HRJ09774.1"
                     /translation="MKSAIPAILIIFACHPSFAADEGIAFFEQKIRPVLIEHCYACHS
                     AEAKKLKGNLYLDSKAGWQKGGDSGEPAILPGKPEESLIIRTVQHLEEDMEMPPKKPK
                     LPDAVIADLVAWVKMGAPDPREGVKVEAKRADKTWWSLQPNNTDLNTAGYIDDHIAAK
                     LKEKGLDFNPPADPRTLIRRMTYDLHGLPPTPEEVEAFVKDCETNTRANKDLIDRLLA
                     SPRYGERYGRHWLDVIRFGESKGFERNVVIDDLWPFRDYVIRSLNEDKTFDQFITEHI
                     AGDVIGKDNPDVEVGSAFLVAGPYDDVGNQDLVAQANIRAATLDDIVTATGGAFLGLT
                     INCARCHDHKFDPVPAEDYYRIKSAFEGVTHERRVIATAEARKAHADALRPLNAELAK
                     LNTERNAHEKSIEARAKAAAASFTHTRPKVDAHGTEEVFAPVTARHLRLVIRNFTTFD
                     QGQTPRPSGGKLTEFEVYDTSGNNVALASHGTTAEGARSAVAEDFPEAYGPQYCIDGK
                     LGEAWFIGSPPILTLTFAKPETVTRINFINARGERGTDESKVRGATPAEYEVQVSMDG
                     ETWTTVASDEGREPATPAHALAKARQSVITAEENARLSDLQKQTAAVQAKINRVPPLP
                     LFWAGTYSQPKEPTRLHKGGDPMKPGDVIVPASLKVLSEVTPAYELPADAPEGERRLA
                     LAKWITQDNPLTARVLANRVWQWHFGTGIVDTPSDFGFLGSQPTHPELLDYLAARLVA
                     NGWRLKELHREILLSKTYQQSAAHRQDGAQADAASRLLWRFPPRRLSAEEVRDTMLAV
                     AGKLQLEPMGGPGFRLYKFTQNNVCTYFPLDTHGPDTYRRAVYHQNARASVVDILNDF
                     DLPDIAFAAPKRANTTTPLQALTLLNHSFTLDMADALARRVDGPEAVVTAYHLLFQRK
                     PNAAESSAAQGLIARHGLKSLCRALLNSNELLHVD"
     gene            8473..9783
                     /locus_tag="PLP58_14455"
     CDS             8473..9783
                     /locus_tag="PLP58_14455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ09775.1"
                     /translation="MKSALCLLPLIAALHAAEPPVCEWVFSAGGSVNDKARAVATDAA
                     GNVFVASECTGDASFGSLQHKSAGGMDMCLVKLDPQGRPLWVSSIGGSKTDRAYGVIA
                     DSAGNAYVTGHFESTDAVVNGMTLPNAGGYDVFTAKFSPEGKVLWLRVKGGEGYDYGH
                     GIAIDSKGKLVVTGAINRQIFCTKYDADGNELWHRVTSGSVSGSGHGIAVDGQDHIII
                     GGNAAGSGAFGKTAIESKTTAALALKLTPDGESVWASLIPGAPSALYHEITCDSQGRV
                     WGVGMFKGSVSVAGGTFQSTGENDNDGLIVHLDAAGRVQWARHLRGPGTDYCLGVATD
                     NQGTAFVCGDFNKDTSLAGHALATRGSGDIFIAAFDAQGGLTWVVQAGGKLNDSAYPL
                     TFRAPAELIIAGSCATSALFGQHEVSHAGAGDLYAAKWRHVSKP"
     gene            9855..11216
                     /locus_tag="PLP58_14460"
     CDS             9855..11216
                     /locus_tag="PLP58_14460"
                     /inference="COORDINATES: protein motif:HMM:TIGR00879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0051119 - sugar transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar porter family MFS transporter"
                     /protein_id="HRJ09776.1"
                     /translation="MPANTPSDPAKLNGYLLGNSLIAALGGLLFGFDTAVISGTTDAL
                     KAVYDLDDNLLGFTVASALIGTILGALFASRPADAIGRRGTLAWLGVFYFVSAAGCAF
                     AWDWWSFIFFRFLGGLAVGGASVVSPLYTAEISPASWRGRMVAVTQFNVVLGILLAYL
                     SNWMIGRLQLGGTEWRWMFGVEAFPALVFFLLVFRTPQSPRWLVAKGRLDEARAVIAR
                     CGTDGGGVDEEIREIQRSLDLEHHSLRERFYSRRYLKPILLAVALAAFNQLSGINAVL
                     YYTPSIFKMAGAGMDSAMLQSVIVGATMFVFTIAAMLVIDRVGRRRLMLLGSIGYILG
                     LAGAAYAFYAHVEGPLLLCSLLVFIAAHGFGQGAVIWVFISEIFPNRVRARGQALGSF
                     THWAMNAAISWTFPLIAAQAGWMAFAFYALCMAGQLVWVVFIMPETKGVSLEKIQEKL
                     GIE"
     gene            11244..12587
                     /locus_tag="PLP58_14465"
     CDS             11244..12587
                     /locus_tag="PLP58_14465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009958671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HRJ09777.1"
                     /translation="MTAYPAAAFIFPLLFLQALDAQPARPNFLVIFTDDHGYGDVSTY
                     HESDVRTPHIDQLAAEGMLFTTMRANCTVCSPSRAALLTGRHPDRAGVPGVIRTQPEN
                     SWGFLDPAVPTLPGELRKAGYHTAIIGKWHLGLESPNTPNERGFDHFHGFLGDMMDSY
                     TTHLRHGRNYMRLNQQAIQPEGHATDLFTEWAAAYLKGGHRPQGRPFFLYLAYNAPHF
                     PIEPPAAWLAKVRARAPGMDPKRAANAAFVEHLDDRIGRVLAALKEAGLEDDTLVVFT
                     ADNGGSLPHAQNNDPWRDGKQSHYDGGLRVPFMVRWPGHVAPGSRSDYAGLNFDIFPT
                     FLELAGLQASAELDAVSLLPVLKGGVIGKPRDLYFVRREGGSAYGGKSYEALIRGDWK
                     LLQNDPWSPLELYNVKDDPLEKNNLAGKAPKVFNELSTALRTHIQRGGAVPWQRP"
     gene            12646..>13163
                     /locus_tag="PLP58_14470"
     CDS             12646..>13163
                     /locus_tag="PLP58_14470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ09778.1"
                     /translation="MIPGWRSSIFHLSRDREWLKRAALIIVAGGFALALVILGVTPAR
                     HDAETPDPLEQALRAEWQELRRAPEPRPRALAGWLRRVTGQMRHLSEVLETAPATWES
                     YQKKGWLLDYDTRPLLEKHTPEPGQRRLLEDFLSARLASDDSGRAAAGARLAEAAELG
                     QPLAGELHAALL"
BASE COUNT         2573 a         4766 c         3594 g         2230 t
ORIGIN      
        1 accgtctgcg gcaagcttgc cgcgctcgcc cgccacgacc tgcaaaacga ggatggctgg
       61 ctgcccaccg agcgcgagct ttccacgcag ctcggcgtca gccgcagcgt cgtgcgcgag
      121 gccgccaagc ggttggaact gcaagggctc atcgaaatcc gccagggcag cggcatgaag
      181 ttcgtggaca agctgcacaa gccgctcagc agcgcgctca acctcctcgt gcccgatgaa
      241 aagcgccgcc tcgtccacct caccgagatc cgccaggctc tggagccgga aaacgcgcgc
      301 ctcgcggcca cccgtgccac ggcatcacaa aagcgcgcgc tgcgccagac gcagaacctc
      361 ctggagcaga cgctcgactt tgaggagcaa atccaggctg acatggactt ccaccgcctg
      421 cttgccgagg cctccggcaa ccccatcgcc gcgcttctca tgcactcgct ctcggacctc
      481 atgcaggcca gcctcgccca cggctaccgc cgagtcaccc cagcccaggc tatcaagcag
      541 cattcaggca ttctccaagc cattgaaaaa cgcgaccccg cagccgcagc ccgcgccatg
      601 cagcgccatc tgctttcggc ttttgccgat ctaggcctcg aacactccca ttgataccgc
      661 catgaacgca tccctggaat tttcccgccg ccagttcctc ggccagatga ccaccggact
      721 caccggtgtg gccctctccc gccttttttc cgccgagtcc aaaacccagc cccacttcac
      781 acccaaggcg aagcaggtgc tgcaaatctt ctgtcctggc gccgcctccc acgtggatct
      841 gtgggatcac aaaccgatgc tggagaaata tgacggcact ccattgcctg gagcggcgga
      901 agtcacgttc cagggcaaaa acggcaacct catgaagtcg ccctggtctt tcagggccgc
      961 cggccagagc ggcaaaagga tctcctccat gctgacgcac atggcgcggc atgtggacga
     1021 catcgccttc atccactcaa tgtccagccg caccaacacc cacggccccg gctgcatcaa
     1081 catgaactcc tgcttcaccc gtgagggctt tccttcagcc ggttcatggg tcagctacgc
     1141 gctggggtca ctgaatgaaa acctgcccac ctacatcgcc attcaggacc tccgtggcga
     1201 gccgccgaac ggaaaggcga actggagcaa cggcttcctc cccgcccagt atcaggctgt
     1261 cgccatggcg gcacagcagc ctttgcgaaa ccttgcgcgc cccgccgcga tcagcagcga
     1321 tgaggaaaag gccacgcgtg atttcttgca gttcatcaac agccagcacg ccgccgcgca
     1381 tcccggcaac agcgaactcc aggcccgcat ggccgcctac gagatggccg cgaaaatgca
     1441 gctcaccgcc cccgaagtca gcgatctctc cggcgaacct caacacgtcc acgatcttta
     1501 cggcaccggc gaccccaaca aactcaaggc cgcctacgcg aagaactgcc tgctcgcccg
     1561 ccgcttcctg gagcagggtg tgcgctacgt cagcctctac tgcgcctccc gcgccagcgc
     1621 cgtggacggc ctgctgaact gggacgctca caaaactctg aaggccgatt acgagcgtca
     1681 ttgccccatc ttcgaccagc ccaccgccgc cctgctcacc gacctcaaac aacgcggcat
     1741 gttgaatgac gtgctcgtcg tctggtgtac cgaatttggc cgcatgccca cgcaccagca
     1801 gggcaccagc ggccgcgacc acaaccccga cgccttcacc acctggatga tgggcgccgg
     1861 aatcaaaggc ggcatcagcc acggcgaaac cgacgaattt ggccgccgcg ccgccgtgga
     1921 tgtcgccacc gtctatgact tctacgccac cgtgctgcac ctgctcggcc tcgatcacga
     1981 aaagctgacc tactaccaca acggcgcaaa gcgccgcctc accgacgtcc acgggcacgt
     2041 cctgacaccc ctgctggcat aaagcacggc acgcatctct caagcatccc atgactcaca
     2101 aacttcactt catcctcttt gcgttccttg cggttgtttg cggccaatcc gccggcgaaa
     2161 agcccaacat cctgctcatc atcacggatg accagggcta cggcgacctg tccatccatg
     2221 gcaacacgca cctggcaact ccgcacctcg acaagctggc tggaacgggc gtgcagtttg
     2281 agcgtttcta cgtcaactca ttctgcgccc ccacccgcgc cgccttgctg acagggcgct
     2341 ggccgctgcg caccggctgt cacggagtca cccacaatcg cgaggccgtg cgcccgtcgg
     2401 aaaccaccct cgccgaggcg ctgcgtcccg cgggctatcg ctccgcctgc atcggaaagt
     2461 ggcacaatgg cgagcagcat ccgttcaccc cgcagggcca gggattcgac gaattcttcg
     2521 gcttcaacaa cggccattgg aatgattact tcgacgccgt cctcctgcgc ggcaccaggc
     2581 acgaagccac ccaaggttac atcgccgacg tgctcacgga tgaggcgatg aagtttatct
     2641 cggcaaacaa atccgggcct tttttctgct acctctccta caacaccccg cactcaccct
     2701 atcaggtgcc ggacaagtac ttcgacaaat tcaaagccaa aggcctcgaa aacaccctgg
     2761 ccgcctttta cggcatgtgc gaaaacattg acgacaatgt gggccgcctc ctcgcgcatc
     2821 tggaagcgga gcatctcgca aaaaacacca tcgtcctttt cctcaccgac aacggcggca
     2881 ccgcaggtgt gaagacctac aacgcaggga tgcgcggcgg caagaccagc gtgcatgagg
     2941 gcggatcgcg cgtgccgctg ttcatccgct ggcctgcggc caactggacg ccccatgtgg
     3001 tcaagcccat cacctcccac accgatcttt atcccaccct gatggacctc tgcggtgtgc
     3061 aagccccctc cggtccgcct gtggacggca cctcgctgcg cccgctgctg gaaaaacccg
     3121 atgccgccgg ctggccggag cgcaccctgt tcacccacaa tcccatcaac gagaccaaca
     3181 agtatcctgg agccgtgcgc acccagaaat accggctcgt gcgtgaaatc accggccccg
     3241 ccggaggctc caaagccaag gccaacgacg ccagcgccaa gccctggcag ctctacgaca
     3301 tggagaatga tcccggccag accaacgaca tcgccagcga gcatcccgaa atcgtcaaag
     3361 accttgccgc tcgatacgac gcctggtttg ccgacatctc caaagaaggg ttgcaacgct
     3421 acccgttgcc cgtcggccat ctcgaacaca accccgtgga actgcacgcg ccgcaggcct
     3481 actttgatcc gcccttgcag ttcgccagcg gccccggctt cgccaacgat tggctcaccg
     3541 cctggaccga tgccgagacc aaagtctggt gggagatcga agccagcgaa gcaggcgact
     3601 acgaactcga actcgcctat ggagcttccc cagctgacgc tggatcacgc atccggctct
     3661 acgctgggaa cagcgccatc gaagtcacct tgccagccgc cgaagccaaa gaaatcccgc
     3721 ttccccaccg cgacgaagct ggcaaagccc gctatcgcaa ccgcgactgg gccacgctga
     3781 aagccggaac tcttaacctc ccaaaaggcc ccgcgaagat cactctcgaa ccgctttcca
     3841 aacccggcgc ccaggtcatg gacctcaaac acctccagat gaaactccag ccaaaagccc
     3901 ccgagaaatg aaggccctcc ctttccttgt cgcgttcttt gcgatccttg gcggccaatc
     3961 ctccgctgca aagcccaacg tcctcttcat tctcgccgat gacctcggct ggggcgatct
     4021 gcgctgctat ggcaatccga ggatagacac ccccgtgctc gactcgctgg cacaggaagg
     4081 cgcgcgattg accgcgcatt actcgccctc gcccctctgc tcgcccgcgc gggcaggttt
     4141 cctcacgggc cgttacaacc accgcaccgg cgcggtggat gtgccgagca atcgcggcct
     4201 ggaccgtctc gatctctctg aaaaaacctt tggcgactac ttccgccatg cgggctgcgc
     4261 caccgcgctc attggcaagt ggcacaatgg cctctactgc cgcgattatc tgcctcatca
     4321 gcgcggcttc gatcttttcc acggctttcc caatggcggc caggactact ggaaatggaa
     4381 tctcctgcgc aatgatgacg ccgtgccgca cgacggacgc tacctctccg acgcgctcaa
     4441 cgacgaaacc ctgaagttca tcaccgctca caaagagaaa cccttcgcca tcttcctcgc
     4501 gcatcatgcg ccgcacagcc cgctgcaagc gcccgaaccg ctcgtggaaa aatatcgcca
     4561 gcgacttggc aaggatgcca gcgaggccgt ggccgtcact tacgccatga tcgaggccat
     4621 ggacaccgga cttgggcgtg tgtttcagac cctcaaagac aacgggcagt gggaaaacac
     4681 gatcatcgtc ttcaccagcg acaacggccc cgtgctcacc aaagacccca agctcggccc
     4741 gcagcaacgc tacaatggcg gcttctcggg agaaaagcag catgtgctcg aaggcggcat
     4801 ccgggtgccc tgcatcgtcg cctggcccgg ccgcatcccg gcaggtcagg tcatcgccac
     4861 gcccgtgcat ggctgcgact ggctgcccac gcttttttcc ctcacccaaa cagacgcgcc
     4921 cgcaggagcc aagcctttcg atggtcagaa catcatgccg cttctcatgg gccagcccgc
     4981 acctgcgctg gccgggcgca ccctcctttt ccaacgcaac cgctacgctc ccgtcgccca
     5041 ctgcaacgcc gccattcgcc agggccgctg gaagctctac tggcccggcg acgactcctc
     5101 actcaaaaaa gacagcgccc gtgacaaccc ctcctaccag cgcggcatca ctcatccgca
     5161 ctgggagatg ccgctcgacc gccagctcga cgcccccacc tccgcgccgc agccagcccc
     5221 gcgtctctac gatctcgatg ctgatcccgc cgagcaaaaa aacctcgcct ctgagcatcc
     5281 cgaaatcgtg cgggcactcg cacaaaaaca cgatgcctgg ttcgcggaaa tcatcccgga
     5341 atggcggcaa tcccgcgccc gcctccttga gcacgaccgc gcctactggg aagaccgccc
     5401 cacacccgac cccgccgcca ttttcgacgg attctggctt tggaaatccg cgccgcagcc
     5461 cgtcaacccc aaaaccgccg acccgctcaa agtcttccgc ggttactgga acacttccca
     5521 aaacgacgag taactccccg cgaacgaatg cttcctggcc tcaccatttg cttccctcga
     5581 ttcatgaaat ctgccattcc agcgatcctg atcatcttcg cttgtcatcc ttctttcgcc
     5641 gcggacgaag gcatcgcctt cttcgagcaa aagatccgac ctgtgctgat cgagcattgt
     5701 tatgcctgtc acagcgccga ggcgaagaag ctgaagggca atctctacct cgactccaag
     5761 gccggttggc aaaaaggcgg cgacagcggc gagcctgcga tccttcccgg caaacccgag
     5821 gaaagcctta tcatccgcac cgtgcagcat ctcgaagagg acatggagat gccgccgaag
     5881 aagcccaagc tgccggatgc cgtaatcgcc gacctcgtgg cctgggtgaa gatgggcgct
     5941 ccagaccctc gtgaaggcgt gaaagtggag gccaagcgcg cggacaaaac gtggtggtca
     6001 ctgcaaccca ataacactga tcttaacacg gctggttaca ttgacgacca catcgccgcc
     6061 aagctcaaag aaaagggact cgacttcaac ccacccgccg atccgcgcac gctgatccgc
     6121 cgcatgacct acgacctgca cggtctgccg ccgacgccgg aggaagtgga ggcgtttgtc
     6181 aaagattgtg agaccaacac ccgcgccaac aaagacctca ttgaccggct gctagccagc
     6241 ccgcgctatg gcgagcgcta cggccgccac tggcttgacg tgatccgctt cggcgagtcc
     6301 aaaggcttcg agcgcaacgt cgtcattgat gacctctggc ccttccgcga ctacgtaatc
     6361 cgctcgctca atgaggacaa gaccttcgac cagttcatca ccgaacacat tgccggcgat
     6421 gtcatcggca aggacaaccc cgatgtcgaa gtcggcagcg ctttcctcgt ggccggtcct
     6481 tatgatgacg tgggcaatca agacctggtg gcacaggcaa acatccgcgc cgccacgctg
     6541 gatgacatcg tcactgccac cggcggcgcc tttctcggcc ttaccatcaa ctgcgcccgc
     6601 tgccacgacc ataaattcga tcccgtcccc gccgaggatt actaccgcat caagtccgcc
     6661 tttgaaggtg tgacacacga gcgccgcgtc atcgccaccg ctgaggcgcg caaagcgcac
     6721 gctgacgcgc tgcgcccgct caatgccgaa ctcgccaaac tcaatacgga acgcaatgcc
     6781 cacgaaaaga gcatcgaagc ccgggccaag gccgccgctg ccagcttcac ccatacccgg
     6841 cccaaagtgg acgcgcacgg cacggaggaa gtcttcgccc cggtcaccgc gcgccacttg
     6901 cgcctggtca tccgcaactt caccaccttc gaccaggggc aaacgccaag gcccagcggc
     6961 ggcaagctca ctgagttcga agtctatgac accagcggaa acaacgtcgc gcttgccagt
     7021 catggcacca cggcggaagg cgcgcgctcc gccgtggctg aggatttccc agaagcctac
     7081 ggcccgcaat actgcattga tggcaagctc ggcgaagcct ggttcatcgg cagcccgccc
     7141 attctcacgc tcacctttgc caagccggaa accgtcaccc gcatcaactt catcaacgcc
     7201 cgcggggagc gaggcacgga cgagagcaag gtgcgcggag ccacgcccgc cgagtatgaa
     7261 gtccaggtct ccatggacgg tgaaacctgg accaccgtgg cctccgacga aggccgcgag
     7321 cctgccactc ccgcccacgc gctggccaaa gcccgccagt ccgtcatcac cgccgaggag
     7381 aatgcacggc tctcggactt gcagaaacaa accgccgccg tgcaggcaaa aatcaaccgc
     7441 gtgccgccgc tgccactctt ctgggccggc acttactccc agcccaaaga gcccacacgc
     7501 ctccacaaag gcggcgaccc catgaaacct ggcgatgtca tcgtgcccgc aagcctcaaa
     7561 gtcctcagcg aagtcacccc cgcctacgag ctgcccgccg acgccccgga aggcgagcgc
     7621 cgtctcgcgt tggcaaaatg gatcacccag gacaatcccc tcaccgcccg cgtgctcgcc
     7681 aaccgcgtct ggcagtggca ctttggcacc ggcatcgtgg atactccaag tgacttcggg
     7741 ttcctcggca gccagcccac acatcccgaa ctgctcgact acctcgccgc ccggctcgtc
     7801 gccaacggct ggcgtttgaa ggaactgcac cgggagattt tgctctcgaa gacgtatcag
     7861 caaagtgccg cgcatcgcca ggacggcgcg caagccgacg ccgccagccg cctgctctgg
     7921 cgctttccgc cccgccgcct cagcgccgaa gaagtgcgcg acacgatgct cgcggtcgcg
     7981 ggcaaactgc aactcgagcc catgggcggc cccggcttcc gcctctacaa gttcacgcag
     8041 aacaacgtct gcacttactt ccccctcgac acccatggcc ccgacaccta tcgccgcgcc
     8101 gtctatcatc aaaacgcccg cgccagcgtc gtggacatcc tcaacgactt cgacctgccc
     8161 gacatcgcct tcgccgcgcc gaaaagagcc aacaccacca ccccgctgca agccctcacc
     8221 ctgctcaacc acagcttcac gctcgacatg gcggacgcgc ttgcaaggcg ggtggacgga
     8281 cccgaggcag ttgtgaccgc ctatcacctt cttttccagc gcaaacccaa cgccgccgaa
     8341 tccagcgccg cccaaggctt gatcgccagg catgggctga aatcactctg ccgtgcgctt
     8401 ctcaactcca atgagctgct gcatgtggat tagccgcgcg aacgggcagc cggggaccgt
     8461 actcctgccc ccatgaaatc cgccctctgt ctcctcccgc ttatcgccgc cctgcacgct
     8521 gccgagccgc ctgtttgcga atgggttttc agtgctggcg gcagcgtcaa tgacaaggcg
     8581 cgcgcggtcg ccacggatgc cgccggcaat gtcttcgtgg cctccgaatg caccggcgac
     8641 gcgtcctttg gcagtctcca gcacaagagc gcgggcggca tggacatgtg ccttgtgaag
     8701 cttgacccgc agggcaggcc gctgtgggtc agcagcattg gcggcagcaa gaccgaccgc
     8761 gcttacggcg tcatcgcaga ttcggcggga aacgcctatg tcaccggcca ctttgaaagc
     8821 accgacgccg tggtgaatgg catgaccctg cccaacgcgg gcggttacga tgttttcacc
     8881 gccaagttct cccctgaagg aaaagtgctc tggttgcgcg tgaaaggcgg agaaggctac
     8941 gattacggcc acggcatcgc cattgactca aagggaaaac tcgtcgtcac cggcgcgatc
     9001 aaccgccaga ttttctgcac caaatacgac gcggatggca atgaactctg gcaccgcgtg
     9061 acttcaggct cagtttcagg cagcgggcac ggcatcgcgg tagacgggca ggatcacatc
     9121 atcatcggcg gcaatgcggc gggctcagga gccttcggca aaacagccat cgaatcaaaa
     9181 acgaccgccg cgctggcgct gaaactcacc cccgatggcg agtccgtctg ggccagcctg
     9241 atcccgggcg cgccgagcgc gctgtatcat gagatcacct gcgattcgca aggccgcgtc
     9301 tggggtgttg gcatgttcaa aggcagcgtg agcgtggcgg gcgggacgtt tcaaagcact
     9361 ggagaaaatg acaacgacgg cctcatcgtg catctcgacg ctgcgggccg cgtgcaatgg
     9421 gcgcgccatc tgcgcggccc tggcaccgac tactgcctgg gcgtggccac ggacaatcag
     9481 ggcacggcct ttgtgtgcgg tgatttcaac aaagacacct ccctggccgg tcacgccctg
     9541 gccacgcgcg gcagcggcga catctttatc gcggcctttg atgcccaagg cggcctcacc
     9601 tgggtcgtgc aggcaggcgg caagctcaac gacagcgcct accctctcac cttccgcgct
     9661 ccggccgaac tcatcatcgc cggttcctgc gcgacatccg ccttgttcgg gcagcatgaa
     9721 gtttcacatg ccggagcagg cgacctgtac gccgcgaagt ggcgtcacgt ctccaaaccc
     9781 tgatctcgcg gcgtcccgga cagaccgcgt gaccgggcgc gttcatctcg atccgccttc
     9841 aacatccacc aaccatgccc gcgaacacgc catccgatcc cgccaagctc aacggctatc
     9901 ttctgggcaa ctccctcatc gccgcgctcg gcggcctgct tttcggcttc gacaccgccg
     9961 tcatctccgg caccaccgac gcgctcaagg cggtctatga tctggatgac aacctgctgg
    10021 ggttcaccgt cgccagcgcc ctgattggca ccatcctcgg cgcgctcttc gccagccgtc
    10081 cggcggacgc catcggccgg cgcggcaccc tggcctggct cggcgtgttt tacttcgtct
    10141 ccgccgcggg ctgcgcgttc gcatgggatt ggtggtcctt cattttcttc cgtttcctcg
    10201 gcggcctggc ggtgggcggc gcctcggtcg tctcgccgct ttacacggcg gaaatttccc
    10261 cggcatcctg gcgcgggcgc atggtggcag tcacccagtt caatgtcgtg ctgggcatcc
    10321 tgctggccta cctctcgaac tggatgattg gccgcctgca gctcggtggc acggagtggc
    10381 gctggatgtt cggcgtcgag gcattccccg cgcttgtgtt tttcctgctc gtctttcgca
    10441 cgccgcagag tccgcgctgg ctcgtcgcca aaggccgact cgacgaggcc cgcgccgtca
    10501 tcgcgcgctg cggaaccgac ggcggcggcg tggatgagga gatcagggaa atccagcgct
    10561 cgctggatct ggagcaccac agcttgcgcg agcggtttta cagccgccgc tatctgaagc
    10621 ccatcctgct ggcggtggcg ctggccgcct tcaaccagct ttccggcatc aatgccgtgc
    10681 tctattacac tccaagcatt ttcaaaatgg ccggcgccgg catggactcc gccatgctcc
    10741 agtccgtcat tgtcggcgcc accatgtttg tcttcaccat cgccgccatg ctcgtcattg
    10801 accgcgtcgg aaggcggagg ctgatgctgc tggggtccat cggctacatt ctgggactgg
    10861 ccggcgccgc ttatgctttt tacgcccatg tggaggggcc gctgctgctt tgcagcctgc
    10921 ttgtgttcat cgccgcgcac gggttcggcc agggcgcggt gatctgggtc ttcatcagcg
    10981 agatcttccc caaccgcgtc cgcgcccgcg gccaggcgct cggcagcttc acacactggg
    11041 ccatgaacgc cgccatctcc tggactttcc cgctcatcgc cgcgcaggcg ggctggatgg
    11101 ccttcgcctt ttacgcgctg tgcatggcgg gccagctcgt ctgggtggtt ttcatcatgc
    11161 ccgagaccaa gggagtctcg ctcgaaaaaa tccaggaaaa gctcggcatc gagtgatcct
    11221 ccaccgccct cgtcaacctc cgcatgaccg catatccagc cgccgctttc atcttcccgc
    11281 ttctcttttt gcaagcgctg gacgcccagc cagccaggcc gaacttcctc gtcattttca
    11341 ctgacgacca cggctacggc gatgtctcca cctatcacga gtccgatgtc cgcacgccgc
    11401 acattgacca gcttgcggcg gagggaatgc ttttcaccac catgcgggcg aactgcaccg
    11461 tctgctcccc ttcccgcgcc gccctgctca ccgggcgcca cccggaccgc gccggcgtgc
    11521 ccggcgtcat ccgcacgcag ccggagaatt cctggggctt tcttgacccc gccgtgccca
    11581 cgctgcccgg cgagctgagg aaggccggct accacaccgc catcatcggc aaatggcacc
    11641 tcggcctcga atccccgaac acacccaacg agcgcggctt cgaccacttc cacggctttc
    11701 tcggagacat gatggacagc tacaccacgc atctgcggca tggccggaac tacatgcggc
    11761 tcaaccagca ggccatccag ccggagggtc atgccaccga tcttttcacc gaatgggccg
    11821 ccgcttactt gaaaggcggg caccgcccgc agggcaggcc cttcttcctt tacctggcct
    11881 acaacgcgcc gcacttcccc atcgagcctc ccgctgcctg gctcgccaaa gtcagggcgc
    11941 gcgcgccggg catggacccg aaacgcgccg ccaatgccgc ctttgtcgag catcttgacg
    12001 accgcattgg ccgcgtgctc gccgcgctca aggaagccgg gttggaagac gacaccctcg
    12061 tcgtcttcac cgccgacaat ggcggctccc tgccccacgc tcaaaacaac gacccctggc
    12121 gcgacggcaa gcagagccat tacgacggcg gcctgcgcgt gcctttcatg gtccgctggc
    12181 cgggccatgt cgccccaggc tcccgcagcg attacgccgg gctcaatttc gacatcttcc
    12241 ccaccttcct ggaactcgcc ggcctccaag cctccgcgga gctcgacgcc gtcagcctcc
    12301 tgcccgtcct caaaggcggc gtcatcggga aaccgcgcga tctgtatttt gtccgccggg
    12361 agggtggctc ggcctacggc ggtaaaagct acgaagccct gatccgcggc gactggaagc
    12421 tgctgcaaaa cgatccctgg tcgcccctcg aactctacaa tgtcaaagac gatcctctgg
    12481 agaagaacaa cctggccggc aaagcgccga aagtgttcaa cgagctttcc accgctttgc
    12541 gaacgcacat ccagcgcggc ggcgccgtgc cctggcagcg tccctgatca ctcagccggt
    12601 ttcaaaacac atgacgaaac cgcccggacg ctccagcatg ggctcatgat tccgggctgg
    12661 cgctcatcca ttttccatct cagccgcgac cgcgagtggc tcaaacgcgc tgcattgatc
    12721 atcgtcgccg gcggcttcgc cctcgcgctg gtcatcctcg gtgtcacccc cgcgaggcat
    12781 gatgcggaga cacccgaccc gctggagcag gcgctgcgcg ccgagtggca ggagttgcgc
    12841 cgcgcgccgg agccgcgccc gcgcgccctg gcagggtggc tgcgcagagt caccgggcag
    12901 atgcggcatc tgagcgaggt gctggagacc gctcccgcca cctgggagtc gtatcagaaa
    12961 aaaggctggc tgcttgacta cgacacgcgt ccgctgctgg aaaaacacac tccggagccc
    13021 ggtcagcggc ggcttttgga ggactttctc agcgccaggc tcgcctccga tgactccggg
    13081 cgcgccgcag ccggcgctcg cctggcggaa gcggcggaac tcgggcagcc gctggcgggc
    13141 gaactccacg ccgccctgct cat
//
LOCUS       DAOMYJ010000144        13131 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Prosthecobacter sp. isolate AMR_MDS_5629
            NODE_1583_length_13131_cov_21.146834, whole genome shotgun
            sequence.
ACCESSION   DAOMYJ010000144 DAOMYJ010000000
VERSION     DAOMYJ010000144.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867850
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prosthecobacter sp. (wastewater metagenome)
  ORGANISM  Prosthecobacter sp.
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Verrucomicrobiaceae; Prosthecobacter.
REFERENCE   1  (bases 1 to 13131)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13131)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.30
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:34:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,742
            CDSs (total)                      :: 4,684
            Genes (coding)                    :: 4,676
            CDSs (with protein)               :: 4,676
            Genes (RNA)                       :: 58
            tRNAs                             :: 55
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13131
                     /organism="Prosthecobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1583_length_13131_cov_21.146834"
                     /isolate="AMR_MDS_5629"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1965333"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..416)
                     /locus_tag="PLP58_14475"
     CDS             complement(<1..416)
                     /locus_tag="PLP58_14475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009965421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HRJ09779.1"
                     /translation="MLSLPSYLAGKAVMTDLWIDVFNPYNNEQVGRVAAIRRDHLEAA
                     IEAHLKNHEPLTRYERSRILNKTRDLLEANREELARLITAEAGLCLRETRYEVGRACD
                     VFAFAAMEALRDDGQTFSCDISPTGKARKIFTLREPL"
     gene            complement(423..1445)
                     /locus_tag="PLP58_14480"
     CDS             complement(423..1445)
                     /locus_tag="PLP58_14480"
                     /inference="COORDINATES: protein motif:HMM:NF028222.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HRJ09780.1"
                     /translation="MNSARVQLFHGPGKPFQMGSAAWPEDLHPGEVLVQISLATICGS
                     DLHTTEGRRTAPTPCILGHEAVGHVVSSARDGVGYGQRVTWTLADSCGHCAPCTRWHL
                     PQKCEHLFKYGHAALSDGSGLNGCYASHIVLRPGTEIMTVPDALPDAIAAPANCALAT
                     IVNALEALPDPCDSALVQGGGLLGIYACAWLRHRGVQEVFCTDLSPQRLAMVAEFGGI
                     PVPHGEKMPQVDLVLEVTGSSAVIPDGVKALRPGGHYVWAGMVHPETQLDLTGEAVLR
                     KCLTLRGIHNYRPEHLAAGLDFLAQNHRQMPFKKLTSPALPLEQLNEGFDLTRSRQWQ
                     RVAIQP"
     gene            complement(1442..2713)
                     /locus_tag="PLP58_14485"
     CDS             complement(1442..2713)
                     /locus_tag="PLP58_14485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009965423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HRJ09781.1"
                     /translation="MKATHHHSPDFLSARRWSLGAVMFCYLFYYTGRQTFGFAIPGIQ
                     AELGMDKQTLGWISAAMLWAYAAGQMINGNLGDKFGGRRMMFLGAFSSFALNWLTSFG
                     VGFKSLATAWGMNGLAQSMGWAPGSRLVSNWFGAHERGRAFGLYVLAAGLSSVLSFIT
                     SLVVLDVLQLDWRWIFRLPVVLMLLGGLVVWIVARDKPADAGFPDTHENEKVFDPDAV
                     KESSWQRYAAALKNGRLLLAGFAIGFQNTVRYGLLIWVPVYFLGDDFKADPLGKWISI
                     ALPIGMALGAVTSGWISDRFCDSRRSGVIASFMILAAVAAMGMYLLPPGHRLGVPVLF
                     LAGFFAYGPQSAFWALAPDLMGRECAGTAVGVMNFFAYAMAGLGEPFIGWMIQHNPFA
                     TTPGVESVALVFPLVAAFAVCSAILAFLIRR"
     gene            complement(2771..3997)
                     /gene="phnA"
                     /locus_tag="PLP58_14490"
     CDS             complement(2771..3997)
                     /gene="phnA"
                     /locus_tag="PLP58_14490"
                     /EC_number="3.11.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009965424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047400 - phosphonoacetate hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0019636 - phosphonoacetate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonoacetate hydrolase"
                     /protein_id="HRJ09782.1"
                     /translation="MFTVNHRTYNPPAKPVVVVCLDGSADEYLDCAVACGRMPNLAKM
                     SVNGWRGFARAAMPTFTNVNNSSIVTGVPPSVHGIGGNFFFDTSTGEEVMMNSSKFLR
                     ADTIFPHAQRAGRKVAVVTAKEKLRDIFASGLISEGGIAFSSEKAKHAVVETHGIGDV
                     EALVGPTPEIYSGDASLYVLKAGVAMLRDGRADFLYLSTTDYMQHKHAPEDPDVLDFY
                     AAIDVEIGHLLALGAVIGITADHGMCAKQKPDGSPNVIYLESELVEQFGEGFRVILPI
                     TDPYVAHHGALGSFAQVHLPEGMDAGKVRDWLLLRPGVTECHERATAARLMELPEDRM
                     GDLVVASGRDVVLGRTPAYHDLSALAGHLRSHGGRYEEMVPLIFSEPLNAKYAARAGS
                     DVRNFDIFEITCNGTH"
     gene            complement(4010..4213)
                     /locus_tag="PLP58_14495"
     CDS             complement(4010..4213)
                     /locus_tag="PLP58_14495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ09783.1"
                     /translation="MLALLELVIAVYQACTATFHMRVALRWMLSSRYRAEIRKDQETL
                     AKVGFGFVFLALLLTVIALLFVF"
     gene            complement(4244..5596)
                     /locus_tag="PLP58_14500"
     CDS             complement(4244..5596)
                     /locus_tag="PLP58_14500"
                     /EC_number="3.1.3.1"
                     /inference="COORDINATES: protein motif:HMM:NF020976.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004035 - alkaline phosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase D family protein"
                     /protein_id="HRJ09784.1"
                     /translation="MIPTFMFGSFSRMLMTPTSSLPRRDFLTGALALGASTLARGADE
                     APAMLGPILGHSDETSAMVWMRAAAAGEFTLEVTPEEGGSPVVMKKVADEKDDLCLHW
                     KVAGLQPGARHRYRILQNGKVIAADAGQILSTAPAAGKPAKVRLAISSCAKEDAGSRA
                     VWNRMASENVDAVVLIGDTPYIDSTELEKQARRHREFAAVPEYQALLKSRPSWWTWDD
                     HDFAGNDASGLAAGKENTRLVFTRYRPQKSYGDGTGGIYTSFRRGPVEVFLLDTRWFS
                     MTEPSFAGSHKPTLLGAKQWEWLQKGLLASTAPFKLLACGVIWDDKENTESDDWGTYK
                     HELTAIQKFIGENKIPGIILIGGDIHASRVLKYKTAKTVGYDLVQFIASPIHGSTIPS
                     LNVYHPDLVRSAVEPNVFLRVDADSTVTPATFHAALINKNGETVFSYHLTEEDLKPKA
                     "
     gene            5704..6258
                     /locus_tag="PLP58_14505"
     CDS             5704..6258
                     /locus_tag="PLP58_14505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009965427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HRJ09785.1"
                     /translation="MNLVELSQRIKQRRLELGLSLEEVAAAAGQTRSWLSKVENFRIT
                     PSLPALARVAEALRAPLSVLLEGLDSKPQIVLTKKKDRRAIQRDPQNSDIVYHSLTHG
                     RASRRMNPFVLDVPAGGGRSISRPHEGEEFLIVLKGRVTLEYGKESYDLDEGDTLYFD
                     ADTPHRLHNPHKKPAQVMCVFLEK"
     gene            6350..7519
                     /locus_tag="PLP58_14510"
     CDS             6350..7519
                     /locus_tag="PLP58_14510"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009961093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent rRNA methyltransferase"
                     /protein_id="HRJ09786.1"
                     /translation="MPAITVQPRARLFHGHVWVYGVEIKARFGDPQPGDVVQLQDVRG
                     KQLGSAIYNPQSQITARLFSYRRQGLDLDFFTRRIQRALRVREEAGVDTALCRVVWSE
                     SDGLPGVVVDRYGDFIVLQTLTLAMARRQDLIIQALVDVFQPRGIIQRNEGAVRKAEG
                     LDQVKGVVWGETPEPYVVRHAGCSFHADLIEGQKTGLYLDQLDNYTHVSRLARDRRVL
                     DCFTNQGGFAQACALAGAAEVTAVDISETAVAQAEKNAAISGAGVRFITANCFDFLKE
                     RESSGAEYDLIILDPPSFTKTKSSVKDALRGYKEIHLRALKMLKPGGIIATFSCSHHV
                     SKGEFHASLVEAGVDVKKTLRRVATYTQRPDHPILASIQETEYLTGYAYEVVAAW"
     gene            complement(7763..8968)
                     /locus_tag="PLP58_14515"
     CDS             complement(7763..8968)
                     /locus_tag="PLP58_14515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009962442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="saccharopine dehydrogenase family protein"
                     /protein_id="HRJ09787.1"
                     /translation="MAKVLIIGAGGVGRVVVHKCAQLPGVFSEILLASRTKSKCDAIA
                     AELSRPIQTAAVDADNVPELAALIRGFQPEVVVNVALPYQDLAIMDACLETGVHYIDT
                     ANYEPRDVAKFEYHWQWAYQDKFKSAGLTALLGCGFDPGVTNVFTAHALKHHFTSIDT
                     LDIIDCNAGDHGKAFATNFNPEINLREVTANGRYWENGSWVETKPLEIKRSFDFPDGI
                     GPKNIYCLYHEELESLTKHIPMRRARFWMTFGDQYIKHMEVLVNVGMTRIDPVMHKGV
                     PIIPIEFLKTLLPEPGTLGPDTKGKTCIGNWIEGTAKDGAFKRLYIYNIKDHEDCYRE
                     TNSQGVSYTTGVPAMIAAKLLLTNPAEYRQPGVWNLEQLNPDPFMTDLNTHGLPWIES
                     FPAHPLPGE"
     gene            9097..10626
                     /gene="gguA"
                     /locus_tag="PLP58_14520"
     CDS             9097..10626
                     /gene="gguA"
                     /locus_tag="PLP58_14520"
                     /inference="COORDINATES: protein motif:HMM:NF040905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HRJ09788.1"
                     /translation="MMILAARRLTKKFPGVTALREVSFDLREGEIHALCGENGAGKST
                     LIKLLSGIHPHGSYEGSFEAGGVEARFAGIADAARAGIAVIYQELALVEEMTVAENIF
                     LGCEPVRWGGFMDWPRMQREAAALLEKFKVALDPAARVADLGVGQKQLVEIVKALAKN
                     SKILILDEPTAALAEHEVRVLLDILRDLRARGIACIYISHKLDEVFAIADRVTVLRDG
                     SSISTRETRATDKARVIQEMVGREIGDLFPRRTAAPGAVLLRARGLSVAGSHGVPRLK
                     DISFELRAGEVLGIGGLMGAGRTELLMHLYGAWGARLAGTVEINGDRLDGLPPARVLR
                     AGLALVSEDRRRYGLVIEQEIGFNLSLSSLAQFTRAGFVSRARETLKNREFFDSLRVK
                     ATGLDAVVGRLSGGNQQKVVLGKALMTGPKVVMLDEPTRGIDVGAKLEIYEIINQLTE
                     AGKAVLLVSSELTELIGMSDRILMLHEGRIGGEFSRAQATQENLMEAAMGHAAAPVMQ
                     A"
     gene            10698..>13131
                     /locus_tag="PLP58_14525"
     CDS             10698..>13131
                     /locus_tag="PLP58_14525"
                     /inference="COORDINATES: protein motif:HMM:TIGR02603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme-binding protein"
                     /protein_id="HRJ09789.1"
                     /translation="MRLPLFCLALCSLVAAAPPTEKRPKEAAPELRMLVPGFEIASLP
                     LKLTNLVNLQYRHDGVLVALGYNGNIHLLRDTDGDGLEDKADLFWQGKGRITAPIGMD
                     LAPKGSPHGDAVYFACKSKVMMVADRDGDGKAEHEQTIAEGWPLAFAGIDTASVCVDP
                     KDGAVFFGLGVRLYNNAYEFDAFGKAQNDLSSERGAILRIAPDFKSREKLCTGVRWPI
                     CLRFNEHGDLFCTDQEGATWLPNGNPFDELLHIERGRHYGFPPRHPRHLPGVIDEPSV
                     HDYGPQHQSTCGFRFNQGVNGGPHFGPDFWKGDALVTGESRGKIYRTKLIKTPAGYVA
                     HNSIIACLNQLAVDLTVTPRGDLLVATHSGDPDWGTGPEGTGHIFVIRYRDKAAPQPV
                     IACATAPDRFEIKHSSAVNSVQEVKIERGLFTKPGDRFETMWPGYEVIKLQRSIPVTE
                     VKAEEVWHYQDHTVITVPKQATSEHFTLQLADDNEIGIEPFGIEIWPGEFDAAWLPHL
                     DPEVSRAVLGRHMPDIHAGHTTTLRTRLDLTGMLQPAIQPGAALDYEYEAEEVTIYLS
                     NTQTAFEALIGGKKFSSQPGQISHDLIHTLVPQRNVPLDLQISFKADAMPDFRITWRT
                     KQDPSRDRAFPLRRFLLPWARPEETPGIATALPRPEIQGGDWQHGRELFFSDRALCSK
                     CHAIRGSGGKMAPDLSNLACRDYKSVFRDIHDPNASLNPEYLTHEVTMKDGTRHSAVM
                     RDEGGEVVLGLGAGVEVRVKRDDIASQKALKTSLMIPQMDQVLGETDFRDLMAFLLTE
                     PPLMKIYSKAEKV"
BASE COUNT         2506 a         4052 c         4216 g         2357 t
ORIGIN      
        1 agcggctcgc gcagggtgaa aatcttgcgc gctttgccgg tgggcgagat gtcgcaggag
       61 aaggtctggc cgtcatcacg cagggcttcc atggcggcga aggcgaacac atcgcaggcg
      121 cggcccacct cgtaacgagt ctctctgagg caaagccctg cctcggcggt gatgagccgc
      181 gcgagttcct cacggttggc ttcgagcagg tcgcgcgttt tgttcagaat gcggctgcgc
      241 tcgtagcgag tgagcggttc gtgattcttg agatgggctt cgatggcggc ttccaagtga
      301 tcgcgcctga tggctgcgac gcggccgact tgctcgttgt tgtaaggatt gaaaacgtca
      361 atccacaagt ctgtcatgac ggcttttccg gcgaggtaac tggggaggga gagcatgatg
      421 aatcaaggtt gaatggcgac ccgctgccat tggcggctgc gggtgaggtc gaagccttcg
      481 ttgagctgct ccagcggcag cgccgggctg gtcagttttt taaagggcat ctgccggtga
      541 ttttgggcaa ggaagtcaag tccggcggcg aggtgttcgg gacggtagtt gtggatgccg
      601 cgcaaggtga ggcatttgcg caagacagcc tcgccagtga gatcgagctg ggtttcagga
      661 tgcaccatgc cggcccagac gtagtggccg ccgggacgca gggctttgac gccatcgggg
      721 atgacggcag agctgccggt gacttcgagc acgaggtcca cctgcggcat tttttctccg
      781 tgcggaaccg gaatgccgcc aaactccgct accatcgcga gacgctgcgg gctgagatcc
      841 gtgcagaaga cctcctgcac ccctcgatgg cgcagccagg cgcaggcgta gatcccaagc
      901 aggccgccac cttgcacgag ggcgctgtcg cagggatcgg gcagggcttc gagcgcgttg
      961 acaatggtgg ccagcgcgca attggccggc gcggcgatgg catccggcaa cgcgtcaggc
     1021 acggtcatga tctcggtgcc ggggcgcagc acgatgtggc tggcgtagca gccgttcaaa
     1081 ccgctgccat cgctgagcgc ggcatggccg tatttgaaaa ggtgctcgca cttctgcggc
     1141 aggtgccagc gtgtgcaggg agcgcaatgg ccgcaggaat cggcaagtgt ccacgtcact
     1201 cgttgtccat agccgactcc atcgcgtgcg ctgctgacga catgtcccac tgcttcatgg
     1261 ccgagaatgc agggcgttgg cgcggtgcgt cggccctcgg tggtgtgcag gtcagagcca
     1321 cagatggtcg cgagcgagat ctgcacgagc acttcgccgg gatggagatc ttcgggccat
     1381 gccgcgcttc ccatttgaaa tggcttgccg ggaccgtgga aaagctggac gcgggcggag
     1441 ttcatcggcg gattaaaaag gcgagaatgg cgctgcacac ggcgaaggcg gcgacgaggg
     1501 ggaagacgag ggcgacgctt tccacgccgg gagtggtagc gaagggattg tgctggatca
     1561 tccagccgat gaagggctcg ccgaggccgg ccatggcgta ggcgaagaag ttcatcacgc
     1621 cgacggcggt gccggcacat tcgcggccca tcaagtccgg tgccagcgcc caaaaggcgg
     1681 actgcggtcc ataagcaaag aagccggcta ggaacagcac gggcacgccc agccgatggc
     1741 ccggagggag caggtacatg cccatggcgg cgacagcggc gaggatcatg aagctggcaa
     1801 tgacgccgct gcggcgcgag tcgcagaatc tgtcggagat ccagccgctg gtcacggcac
     1861 ccagtgccat cccgatgggc agtgcgatgc tgatccattt gccaagcgga tcggctttga
     1921 agtcgtcacc aaggaaatag acaggcaccc agatgagcag gccgtagcgc acggtgtttt
     1981 gaaaaccgat tgcgaatccg gcaagcagca gccttccgtt tttcagcgcc gctgcgtaac
     2041 gctgccagga ggattctttg acggcatcgg gatcgaagac cttttcattc tcgtgcgtgt
     2101 ccgggaagcc agcatcggca ggcttgtccc gcgcgacgat ccacaccacc agtccgccga
     2161 gcagcatcag gaccaccggc agccggaaga tccagcgcca gtcgagctgg aggacgtcca
     2221 gcaccaccaa ggaggtgata aatgagagca cggaggacaa gccggccgct agcacataaa
     2281 ggccgaaggc gcgcccgcgt tcgtgcgcac cgaaccagtt cgacaccaat cggctgccgg
     2341 gtgcccagcc catgctttgc gccagaccgt tcatgcccca ggcggtggcg agcgatttga
     2401 aaccgacgcc gaaactggtg agccagttca gggcaaagga gctgaacgca ccgaggaaca
     2461 tcatgcggcg gccgccgaat ttgtcgccga ggttgccatt gatcatctgc ccggcggcgt
     2521 aggcccagag catggcagcg ctgatccagc cgagggtttg tttgtccatg ccaagctccg
     2581 cctggatgcc gggaatggcg aagccgaaag tttggcgacc cgtgtaataa aacaggtagc
     2641 agaacatcac cgcacccagc gaccaacggc gggcgctgag gaagtctggg gagtgatggt
     2701 gagtggcttt catagggtgg ggcaagcttc cagcttgcca gtgagtgcaa cctggaaggt
     2761 tgcaccacca tcaatgcgtg ccgttgcagg tgatctcaaa gatgtcgaag ttgcgcacgt
     2821 cgctgccggc gcgggcggca tacttcgcat tgagcggctc gctgaagatg agcggcacca
     2881 tttcctcata gcggccgccg tggctgcgga gatggccggc gagcgcgctg aggtcgtgat
     2941 aggcgggcgt gcggcccagg acgacatcgc gtccgctggc gacgacgagg tcgcccatgc
     3001 gatcctcggg cagctccatc aaacgcgcgg cggtggcgcg ttcgtggcat tcggtgacac
     3061 cgggacggag cagcagccag tcgcgcacct tgcccgcgtc catgccctcg ggcagatgca
     3121 cttgggcgaa ggatccgagc gcgccgtgat gcgctacata aggatcggtg atgggcagga
     3181 tgacgcggaa gccctcgccg aactgctcca cgagttcgct ttcgagatag atcacgttcg
     3241 ggctgccatc cggcttttgt ttggcgcaca tgccgtggtc ggcggtgatg ccgatgacgg
     3301 cgccgagggc gagcaggtga ccgatttcga catcaatggc cgcgtagaaa tcgagcacat
     3361 cgggatcttc cggcgcatgc ttgtgctgca tgtagtcagt ggtgctcagg tagaggaaat
     3421 cagcgcggcc atcgcgcaac atcgccacac cggccttcaa aacatagagg ctggcgtcgc
     3481 cgctgtagat ttccggcgtg gggccgacga gcgcctcgac atcgccgatg ccgtgggtct
     3541 ccacgaccgc gtgtttggct ttctcggatg aaaaagcgat gccgccctcg ctgatgaggc
     3601 cgctggcgaa aatgtcgcgc agtttctcct tcgcggtgac cacggccacc ttgcgcccgg
     3661 cgcgctgggc gtgggggaaa atagtgtcgg cgcggaggaa cttggaagag ttcatcatca
     3721 cctcctcgcc cgtgctggta tcgaagaaga agtttcctcc gatgccatgc acgctgggcg
     3781 gcacgccggt gacgatgctg ctgttgttca cgttggtgaa ggtcggcatg gcagcgcgcg
     3841 caaagccccg ccagccgttc acgctcatct tcgcgaggtt cggcatgcga ccgcacgcga
     3901 cggcgcagtc gaggtattca tccgccgaac catcgagaca gacgacgacg acaggcttgg
     3961 cgggcggatt gtaggtgcgg tggttgacgg tgaacatggc gaggtggtgt tagaaaacaa
     4021 aaagcagggc gatgacggtc aacagcaggg cgagaaagac gaaaccgaac ccgactttgg
     4081 ccagggtctc ctggtccttg cgaatctcgg cacgatagcg cgaggaaagc atccaacgaa
     4141 gcgccactct catgtggaag gttgccgtgc aggcctgata gactgcgatg acgagttcca
     4201 gaagagccag cataaaagag ggaggttttg ttccaacaga ggatcaggct ttgggcttca
     4261 aatcctcctc ggtcagatgg tagctgaaga cggtctcgcc attcttgttg atgagcgcgg
     4321 cgtggaaggt ggcgggcgtg acggtgctat cggcgtccac gcggaggaag acgttgggtt
     4381 ccacggcgct gcggacgagg tcggggtgat agacgttcag ggaggggatc gtgctgccgt
     4441 ggatggggga ggcgatgaac tgcacgaggt cgtagccgac ggttttggcc gttttgtatt
     4501 tgagcacgcg gctggcgtgg atatcgccgc caatgaggat gatgccgggg attttatttt
     4561 cgccgatgaa cttctggatg gccgtcagct cgtgcttgta ggtgccccag tcgtcggact
     4621 ccgtgttttc cttgtcatcc cagatgacgc cgcaggccag cagcttgaag ggtgcggtgg
     4681 aggcaagcag gcctttttga agccattccc attgtttcgc accgagcagg gtgggtttgt
     4741 gcgaaccggc aaaggagggc tccgtcatgg aaaaccaccg ggtgtcgagc aggaagacct
     4801 ccaccggccc gcgccggaaa ctcgtgtaga tgccgccggt gccatcgccg tagcttttct
     4861 gcggccggta gcgggtgaaa acgagacgcg tattttcttt gcctgccgca agcccgctgg
     4921 catcgttgcc cgcgaaatcg tgatcatccc acgtccacca gctggggcgc gatttcagga
     4981 gtgcctgata ctccggcacg gcggcgaact cacgatgccg ccgcgcctgc ttctccagct
     5041 ccgtgctgtc aatgtaaggt gtgtcgccga ttaaaacgac ggcatccaca ttctccgacg
     5101 ccatgcggtt ccacaccgcg cggctgcccg cgtcctcctt ggcgcatgag ctgatggcga
     5161 gccgcacttt cgcgggtttg ccggctgccg gagccgtgga caatatctgc ccggcatcgg
     5221 cggcgatcac cttgccattt tgcaggatgc gataccgatg cctggcgcct ggctggaggc
     5281 cggccacctt ccagtgaagg cagagatcgt ctttttcatc cgccaccttc ttcatcacca
     5341 cgggcgatcc gccctcctcc ggcgtgactt ccagcgtgaa ctcaccggcg gccgccgcgc
     5401 gcatccagac catggcggag gtttcgtcgg aatgtccgag gatggggccg agcattgcgg
     5461 gggcttcatc cgcgcctctt gcgagcgtgg aggcacccag cgccagtgct ccggtcaaaa
     5521 aatcacgacg aggcagggaa gaagttggcg tcataagcat gcgggagaac gacccaaaca
     5581 tgaaggtcgg aatcatcctc agagcatgat tttacccgcc acgattttgt cgtgactcca
     5641 tcgctagcca gaaacatgtt gcctgacaag aaacttttga gacaattaag caacacatct
     5701 ttcatgaacc tcgtcgaact ctcccagcgc atcaaacaac gccgcctcga actcggcctc
     5761 agtctggagg aggtcgccgc cgccgccggg cagacgcgga gctggctctc gaaggtggaa
     5821 aacttccgca tcacgccctc gttgcccgcg ctggcccgcg tggccgaggc gctgcgcgcg
     5881 ccgctctccg tgctgctgga ggggctcgac tcgaagccgc agatcgtttt gaccaaaaag
     5941 aaggaccgcc gcgccatcca gcgcgatcca cagaattcgg acattgtcta tcacagcctc
     6001 acccatggcc gcgccagccg ccgcatgaac ccgttcgtgc tggatgtgcc cgccggcggc
     6061 ggccgcagca tctcgcgccc gcatgagggc gaggaattcc tcatcgtgct caaaggcagg
     6121 gtcacgctcg agtatggcaa ggagagctat gatctcgacg agggcgacac gctttacttc
     6181 gacgccgaca ccccgcaccg cctgcacaac ccgcacaaaa aacccgcgca ggtgatgtgc
     6241 gtgtttttgg agaagtgatg tccaccagcc cgcttgaatt gcggctggca ttcccggcaa
     6301 atccggcgca gctatggccc cgttttcagc catcccattt catccagcca tgcccgccat
     6361 caccgtccag ccccgcgccc gtctttttca tggtcatgtc tgggtctatg gcgtggagat
     6421 caaggcgcgc ttcggcgatc cgcagccggg ggatgtggtg cagctccagg atgtgcgcgg
     6481 caagcagctc ggcagcgcca tttacaatcc gcagtcgcag atcaccgcgc gcctcttttc
     6541 ctaccgccgc caggggctgg acctggactt cttcacccgg cgcatccagc gcgcgctgag
     6601 ggtgcgcgag gaggccggcg tggacaccgc gctctgccgc gtggtctgga gcgagtcgga
     6661 cggcctgccc ggcgtcgtcg tggaccgcta cggcgacttc atcgtgctgc agacgctcac
     6721 gctcgccatg gcgcggcggc aggacctcat catccaggcg ctggtggatg tcttccagcc
     6781 gcgcggcatc atccagcgca atgagggcgc cgtgcgcaag gccgaggggc tggatcaggt
     6841 caaaggcgtg gtctggggcg agacacccga gccttatgtg gtgcggcacg ccggctgctc
     6901 cttccatgcc gacctcattg aggggcaaaa aaccggcctc tatctggatc agctcgacaa
     6961 ttacacccac gtctcacgcc ttgcgcgcga ccggcgcgtg ctggactgct tcacaaacca
     7021 gggcggcttt gcccaggcct gcgccctggc cggcgcggcg gaggtgacgg cggtggacat
     7081 cagcgagacc gccgtggcgc aggcggagaa aaacgccgcc atctccggcg cgggcgttcg
     7141 tttcatcacg gcaaactgtt ttgacttcct caaagagcgg gagtcctccg gcgcggagta
     7201 tgacctcatt atcctcgacc cgccctcctt cacgaagacg aagtcctccg tcaaagacgc
     7261 cctgcgcgga tacaaggaga tccacctgcg cgcgctgaaa atgctcaagc ccggcggcat
     7321 catcgccacc ttcagttgca gccaccacgt cagcaaaggc gagttccacg ccagcctcgt
     7381 cgaggccgga gtggacgtga aaaagaccct gcgccgcgtg gccacctaca cccagcggcc
     7441 ggaccacccc atcctcgcca gcatccagga gacggaatac ctcaccggtt atgcgtatga
     7501 ggtcgtggcg gcttggtgag aggtttgtca tggcagcggc aagccagacc gggagcgggc
     7561 tacaccaagc cgttgcgtga catcagattc ttggaggtcg agttttttga tcaaccgaca
     7621 ccatccttta cagccatttg cctgcgcaaa aacgcgcgcg actgcgtgaa cacggtctcc
     7681 aaagaaaggg gaagccatat atgcaatctg ccgtcacgcg cgttcgggaa acagcattcg
     7741 accagttcct atgcagcacg cctcactcac ccggcaaggg gtgggcggga aagctttcga
     7801 tccagggcag gccgtgagtg ttgaggtcgg tcatgaaggg gtcgggattg agctgctcca
     7861 gattccagac accgggctgg cggtattcag cgggattggt gaggagtagc ttcgcggcga
     7921 tcatggcggg cacgccggtg gtgtagctga cgccctggct gttggtctcg cggtagcagt
     7981 cctcgtggtc tttgatgttg tagatgtaga ggcgcttgaa ggcgccgtcc ttggccgttc
     8041 cctcgatcca gttgccaatg caggtcttgc ccttggtatc gggccccagc gtgccgggct
     8101 caggcagcag ggttttcaga aactcgatgg ggatgatcgg cacgcctttg tgcatgacag
     8161 ggtcaatgcg cgtcataccg acgttcacga gcacttccat gtgtttgata tactggtcgc
     8221 caaaggtcat ccagaagcgt gcgcgacgca tggggatgtg cttcgtgaga ctctccagct
     8281 cctcgtggta gaggcagtag atgtttttcg ggccgatgcc gtccgggaag tcgaagctgc
     8341 gcttgatctc aagcggcttc gtctccaccc aggagccgtt ttcccagtag cgcccgttgg
     8401 cggtgacctc gcgcaggttg atctcggggt tgaagttggt ggcaaaagcc ttgccgtggt
     8461 cgcccgcgtt gcagtctatg atgtcgaggg tgtctatgct ggtgaagtgg tgcttcaagg
     8521 cgtgcgccgt gaacacattg gtgacgcccg ggtcgaagcc gcagccgagc agggcggtga
     8581 ggccggcgga tttgaatttg tcctggtagg cccactgcca gtggtattcg aacttggcga
     8641 catcgcgcgg ctcgtagttc gccgtgtcaa tgtaatgcac gccggtctcc aggcaggcat
     8701 ccatgatggc caggtcctga tagggcagcg ccacattgac gacaacctca ggctggaagc
     8761 cgcggatcag cgccgccagc tccggcacat tgtccgcgtc caccgccgcg gtctggatcg
     8821 ggcgggaaag ctccgccgct atggcgtcac acttcgactt ggtgcgggag gccagcagga
     8881 tttcactgaa cactccggga agctgcgcgc atttgtggac gacgaccctg ccgacgccgc
     8941 cggcgccgat gatgagaact tttgccatag ggcgcgcagg gtggcgtctc gcacggcgga
     9001 gtcaagcggc gcggtgtcaa ctttggggca cacgagcgga caggcgcggc cgcattcatc
     9061 ctggcgcgcg cggcgcggtt cgttatcatc acgcgcatga tgattctcgc cgcgcgccga
     9121 ctgaccaaga aattccccgg agtcaccgca ttgagggagg tttccttcga tctgcgcgaa
     9181 ggcgaaatcc acgcgctgtg cggcgagaac ggcgcgggaa aaagcacgct catcaagctg
     9241 ctctccggca tccatccgca cggcagctac gagggcagct ttgaggcggg cggcgtggag
     9301 gcgcgtttcg ccggcatcgc cgacgcggcg cgcgcgggca tcgccgtcat ttaccaggag
     9361 ctggcgctgg tggaggaaat gacggtggcg gagaacatct ttctcggctg cgagccggtg
     9421 cgctggggcg gcttcatgga ctggccgcgc atgcaacgcg aggccgcggc tctgctggaa
     9481 aaattcaagg tggcgctgga ccccgccgcg cgcgtggctg atctcggcgt gggccagaag
     9541 cagctcgtgg agatcgtgaa ggccctggcc aagaactcga aaatcctcat cctcgacgag
     9601 ccgaccgccg cgctggcgga gcatgaggtc cgtgtgctgc tcgacatcct gcgggatctg
     9661 cgcgcgcgcg gcatcgcctg catctacatc agccacaagc tcgacgaggt cttcgccatc
     9721 gcggaccgtg tcaccgtgct gcgcgacggc tccagcatca gcacgcgcga gacgcgcgcg
     9781 acggacaagg cgcgtgtcat ccaggaaatg gtgggccgcg aaattgggga cctttttccc
     9841 cgccgcaccg ccgcgcccgg agccgtgctg ctgcgcgcgc gcggcctcag cgttgccggc
     9901 tcgcacgggg tgccccggct gaaggacatc agcttcgagc tgcgcgcggg cgaggtgctc
     9961 ggaatcggcg gcctgatggg cgcggggcgc acggaactgc tcatgcacct ctatggcgcc
    10021 tggggcgccc ggctggccgg caccgtggag atcaacggcg accggcttga cggcctgccg
    10081 cccgcgcgcg tgctgcgcgc cgggctggcg ctggtgagcg aggaccgccg gcgctacggc
    10141 ctggtcatcg agcaggagat cggcttcaat ctttccctct cctctctggc acagttcacc
    10201 cgcgcgggtt tcgtgagccg cgcgcgggag acgctgaaaa accgcgagtt ttttgactcg
    10261 ctgcgggtca aggccaccgg cctcgacgcc gtggtgggca ggctctccgg cggaaaccag
    10321 cagaaggtgg tgctgggcaa ggcgctgatg acagggccaa aggtcgtcat gctcgacgag
    10381 ccgacgcgcg gcatagatgt gggcgcgaaa ctggaaatct acgagatcat caaccagctc
    10441 accgaggccg gcaaagccgt gctgctcgtc tccagcgagc tgaccgagct gatcggcatg
    10501 agcgaccgca tcctcatgct ccacgagggc cggatcggag gcgaattcag ccgcgcgcaa
    10561 gccacccagg aaaacctcat ggaggccgcc atgggccacg ccgcggcacc tgtcatgcag
    10621 gcttgaaagc acgaccaggg tgggcggttc gttttgcgtc agggataatc cttgtccacg
    10681 cgccccgttg taaactcatg cgcctcccgc ttttctgcct cgctctttgc tcgctcgtcg
    10741 ccgccgctcc tcccacggaa aagcggccga aggaggccgc gccggagctg cgcatgctcg
    10801 tgcccggctt tgagatcgct tccttgccgc tgaagctcac aaacctcgtc aatctccaat
    10861 accgccacga cggcgtgctc gtcgcgctcg gttacaatgg caacatccac cttctgcgtg
    10921 acacggatgg cgacgggctg gaggataagg ccgatctctt ctggcagggc aaaggcagga
    10981 tcaccgcgcc catcggcatg gacctcgcgc cgaaaggctc gccgcatggc gacgcggtct
    11041 actttgcctg caaaagcaag gtcatgatgg tcgccgaccg cgatggtgat gggaaggctg
    11101 agcatgagca aaccatcgcc gaaggctggc cgctcgcctt tgccggcatc gacaccgcga
    11161 gcgtgtgtgt ggatccgaaa gacggcgcgg tcttcttcgg cctgggcgtg cgtctttata
    11221 acaacgccta cgagttcgat gccttcggca aggcgcaaaa cgacctcagc agcgagcgcg
    11281 gggccatcct gcgcatcgcg ccggacttca aaagccgcga gaaactctgc accggcgtcc
    11341 gctggcccat ctgcctgcgc ttcaacgagc acggcgacct tttctgcacc gatcaagaag
    11401 gcgcgacctg gctgccgaac ggcaatccct ttgacgaact gctccacatc gagcgcggca
    11461 ggcactacgg cttccctccg cgtcatccac ggcatctgcc cggcgtgatt gatgagccca
    11521 gcgtccatga ctacggcccg cagcaccaga gcacctgcgg cttccgcttc aatcaaggcg
    11581 tcaacggcgg cccgcacttc ggcccggact tttggaaagg cgatgcgctt gtcaccggcg
    11641 agtcacgcgg caaaatctac cgcaccaagc tcatcaagac accggcgggc tacgtcgcgc
    11701 acaacagcat catcgcctgc ctcaaccaac tcgccgttga tctcaccgtg acaccgcgcg
    11761 gcgacctcct cgtcgccacc cacagcggcg atcccgactg gggcaccggt cccgaaggca
    11821 ccggccatat cttcgtcatc cgctaccgag acaaagccgc cccgcagccc gtgatcgcct
    11881 gcgccaccgc gccggatcgc tttgagatca agcacagcag tgccgttaat tcggttcaag
    11941 aagtcaaaat cgagcgcggc ctcttcacca agcccggcga ccgctttgaa accatgtggc
    12001 ccggctatga ggtcatcaaa ctccagcgct ccataccggt gactgaagta aaagcggagg
    12061 aagtctggca ttatcaagat cacacagtca tcaccgtgcc caaacaggcc acctccgagc
    12121 acttcaccct ccaacttgcc gacgacaacg aaattggcat cgaacctttc ggcatcgaaa
    12181 tatggcccgg agaatttgat gccgcatggc tgccacatct cgatcctgaa gtctcccgcg
    12241 ccgtgctcgg caggcacatg cctgacattc acgcaggcca caccaccacc ctccgcaccc
    12301 gcctcgacct caccgggatg ctccagcccg ccatccagcc cggagccgcg ctcgactacg
    12361 aatacgaggc cgaagaagtc acgatctacc tctccaacac gcaaaccgcc tttgaagccc
    12421 tcatcggcgg caagaagttc agctcacagc ctgggcagat cagccacgac ctcattcaca
    12481 cgctcgtgcc gcagcggaat gtgccgctcg atttgcagat cagcttcaaa gcggatgcca
    12541 tgcccgactt ccgcatcacc tggcgcacga agcaggaccc ctcgcgtgat cgcgcctttc
    12601 cgttgcgacg ctttttgctg ccgtgggcca gaccggagga gacgccgggc atcgccaccg
    12661 cgttgccgag gccggaaatc cagggcggcg actggcagca cggtcgcgag ctgtttttca
    12721 gcgatcgcgc cctgtgctcc aaatgccacg ccatccgcgg cagcggcggc aagatggctc
    12781 ccgacctcag caacctcgcc tgccgcgatt acaaaagcgt cttccgcgac atccatgatc
    12841 cgaacgcctc gctgaacccc gaatacctca cgcacgaggt caccatgaag gacggcacgc
    12901 ggcacagcgc cgtcatgcgc gatgaaggcg gcgaagtcgt gctcggactc ggcgcaggcg
    12961 tcgaagtgcg agtgaagcgc gacgacatcg cctcgcaaaa agccctcaag acctcgctca
    13021 tgatcccgca gatggaccag gtgctcggcg agacggattt tcgtgatttg atggcctttt
    13081 tgctcaccga gccgcctttg atgaaaattt actcgaaggc tgaaaaagtc c
//
LOCUS       DAOMYJ010000145        13121 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Prosthecobacter sp. isolate AMR_MDS_5629
            NODE_1586_length_13121_cov_21.040793, whole genome shotgun
            sequence.
ACCESSION   DAOMYJ010000145 DAOMYJ010000000
VERSION     DAOMYJ010000145.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867850
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prosthecobacter sp. (wastewater metagenome)
  ORGANISM  Prosthecobacter sp.
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Verrucomicrobiaceae; Prosthecobacter.
REFERENCE   1  (bases 1 to 13121)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13121)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.30
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:34:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,742
            CDSs (total)                      :: 4,684
            Genes (coding)                    :: 4,676
            CDSs (with protein)               :: 4,676
            Genes (RNA)                       :: 58
            tRNAs                             :: 55
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13121
                     /organism="Prosthecobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1586_length_13121_cov_21.040793"
                     /isolate="AMR_MDS_5629"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1965333"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..260)
                     /locus_tag="PLP58_14530"
     CDS             complement(<1..260)
                     /locus_tag="PLP58_14530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009963809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HRJ09790.1"
                     /translation="MDLRHLRYFQAVAEEMNFSRASKRLRIAQPALSRAVQEIEHELG
                     ARLIERGRRTLSLTSAGEVLLHEAAADGSLISSMSIFWCAAIG"
     gene            complement(375..716)
                     /locus_tag="PLP58_14535"
     CDS             complement(375..716)
                     /locus_tag="PLP58_14535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009962330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family protein"
                     /protein_id="HRJ09791.1"
                     /translation="MIPAKTILLLLLSNVFMTFAWYAHLRDLRSKPLMLAIALSWGIA
                     FFEYCFQVPANRIGHAVFTLPQLKILQEVITLTVFVPFAVYYMRTPLTWNYLWAGLCL
                     MGAVYFIFKPQ"
     gene            987..1829
                     /locus_tag="PLP58_14540"
     CDS             987..1829
                     /locus_tag="PLP58_14540"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015411.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HRJ09792.1"
                     /translation="MNPPVQQRPGLGIIGMGLMGGALMRMTGAARGWDIDPARCVHAG
                     SAAEVFANCAVVMLCLPDSHIASAVLAAAETRPGQIIVDTTTGDPGVMAALGAALAEK
                     GCHYLDATISGSSVQLLRRDVLVMAGGDADVVERCREIFDTFASEVIHAGPCGAGARM
                     KLVTNLVLGLNRAALAEGLAFAEALGVEPESALRAMRAGNAYSRAMDVKGEKMIRRDY
                     TPQARLSQHLKDLRLMQNCAGERGMDLPLARTHRALLEKAEGMGLGSLDNSAILEVLR
                     LHRE"
     gene            1826..3073
                     /locus_tag="PLP58_14545"
     CDS             1826..3073
                     /locus_tag="PLP58_14545"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HRJ09793.1"
                     /translation="MNPAPLNRRARHAVLAAAFLGLVFDGVELGLMPVASLSVSQSLL
                     GEAYTPTLGGDWFARFTAALMLGAAIGGIALGALGDRVGRTRAMGVSILFYSLFAAMG
                     SLARSQEQMLLLRFLVGLGVGGMWPNGIALVSECWPRASRPVVSGVMMAGLNAGILLL
                     SQLARHWPVTPESWQWIFKLAGVPALLGIVVLLALPESPQWLAARAARKTPVTPLREL
                     FQPGMARVTLAGILISAVPMVGAWAASKWMIPWADKVAGGADAGYKAATQGWWALGAV
                     CGSFMGAQLAARMGRRRSYLLISICATALTLAMFQLTAPLRPGFHAVVCAQGFAATLF
                     FGWLALYLPELFPTRVRATGSGLAYNSGRFATAAGVLLAGFLFSALGGDYARVGAWCA
                     LIYAFGIIAIWFAPDTGKTPRPE"
     gene            complement(3077..4564)
                     /gene="lysS"
                     /locus_tag="PLP58_14550"
     CDS             complement(3077..4564)
                     /gene="lysS"
                     /locus_tag="PLP58_14550"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009963802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HRJ09794.1"
                     /translation="MQETEHTESELLQFRRAKLDELQKRGIAAFGGRFEVTNDPGTLK
                     RQLTEGIVAEGVEVRIAGRVLSRREMGKATFFDVGDITGRIQCYLSKGDVGDDAYALF
                     IHQMDIGDFVGVEGRTFVTKRGERSVHVAALTPLSKALRPLPDKWHGVTDKEIKYRQR
                     YLDLVSNERSGQLFVTRSKMIAEIRAYLHGRDFLEVETPMMQDVPGGAAARPFETHFN
                     ALDQKMYLRIAPELYLKRLLVGGFNRVFELNRNFRNEGLSRRHNPEFTMLEAYWAYAD
                     FEQMADLVEGMICALAEKFCGGLVIEHKDEEGGVTRTIDLTRPWKRAPYRELVRGAAG
                     DDWFSLDKEAKVARAQSLGVQEIHAGDEHYELDQKVFEKLVEEKSFNPVFVTHVPKEL
                     VPLAKQNAQDDSVVDVYELVINGQEISPGYSELNDPIVQRERLEHQAGEEVQKIDEEF
                     ILALEHGMPPAGGIGIGIDRLIMMLTGAESIRDVILFPQLKRKEA"
     gene            complement(4633..5409)
                     /locus_tag="PLP58_14555"
     CDS             complement(4633..5409)
                     /locus_tag="PLP58_14555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009960534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HRJ09795.1"
                     /translation="MRLQDQIILITGSTTGIGAAMARRFAQEGARVIVHGRDTARGEA
                     LRQELGTDRAAFIAGDLADAAHPDELAAAAVAAFGRLDALVNNAALTTRARITETDLV
                     FFDRMVAVNLRAPFMLIRALLPALKESQGSVLNIGSILAHCGQSNLPVYSMTKGGLMT
                     LTRNLANSLGRDRVRVNQMNVGWTLTENEYHTKVGDGLPPDWPDHLPPVAAPFGRLLS
                     PEDIAEAAVYWVSSASSPVSGSVFEIEQYPLIGRIADQEG"
     gene            5531..6421
                     /locus_tag="PLP58_14560"
     CDS             5531..6421
                     /locus_tag="PLP58_14560"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HRJ09796.1"
                     /translation="MTRLRADLMLLACAAVWGVAFICQKRAMAHIGPFLFIALRAWLA
                     CAALAPLAWREWKKEGARVNPRRFLRLSLLAGLAFFGGAAFQQAGLVTATVTNAGFLT
                     ALYVVITPFLAWAFMRQKPGPQVWAAAVLSFGGTWLLGGGSISALSSGDVLVAVSAVL
                     WALHVVLVGLAAGGGMAAAFTGAQFVVVGTLAGLAAAFLEEAGFDAIWQAGPDILYVG
                     VLSSALTFTLLAVALRATRPSEAAVILSTESLFAAFGAYVLLGERLTTTGWLGAAAIF
                     AATLLVHAPVRATRRTQPPG"
     gene            complement(6423..9251)
                     /locus_tag="PLP58_14565"
     CDS             complement(6423..9251)
                     /locus_tag="PLP58_14565"
                     /EC_number="6.5.1.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA ligase"
                     /protein_id="HRJ09797.1"
                     /translation="MPPLIEVTHRRGIYLPEADLWLDPHFGVERAFISHAHSDHFARH
                     RLTFCSEVTRHIMRARYGEKETAEFQMLPLRQDLEIHGWRLRLYPAGHIVGSAMLHLT
                     RLSDGATLLYTGDYKLRHGLSAEPCELPRADVLVMECTFGLPQFRFPPHAETEAAVLR
                     WVRETLEDGGIPVLLGYSLGKAQEILRILAGSGYPVMLHKSVLEMTQAVAPVIGALPE
                     HRLFDAAEASGHVLVFPPSANRSQPLRKLKVCRTAMLSGWAMQPGARYRYQVDEAFPL
                     SDHADYDELLQTVETVNPKRVWLVHGSTREFAADLRARGWEAWTLEKEDQLEFLKFED
                     RSSWGTGVLPVLHAAPDAEPSSKFHPSDFAAWALTCARTAAEASRLKKTALLAEYLRS
                     LPPDELALAARYCSGLVFDPAAGEAPLNTGWAVFRRALQELAGISEAEYRVISRSQAD
                     AGRTAFLMLGMRGAQAPMNTPLTLTGTDAFFRHLREARGPVLKTRLLRECLAASDAET
                     GSWLVRLMTGELRMGSREGLIEEAIAQAFDQDADSVREAAMLCGDIGRAAQLAQAGRL
                     HEAAPRLGVPVKVMLASPEETAEDIWERLGEDRGSKFEARNSKTEESTVSAIAPFPVL
                     QTSNFEFRTSPTVWLEDKFDGIRAQLHRGANRAEIYTRDLKPVGFQFPEILDAARMMR
                     DEVILDGEIIAYAEDKKLSFFDLQKRLGRRDQADLFLPSDIRVRYVVFDVLWRNGASL
                     LERPLSERRAELEAVALPEPMGVIEIWQAASPFDIEAAFHAARRRGNEGLIAKDPAAA
                     YRPGRRGKSWLKLKKAFATLDVVVVKAEQGHGKRSHVLSDYTFAVRDEAGALRVIGKA
                     YSGLTDAEIEELTAHFTSNTLEQKGRVRTVLPDTVLEIAFDSIQPSKRHDSGLALRFP
                     RIKAIRRDKTPGEIDTLETARRLAGLPG"
     gene            complement(9370..11805)
                     /locus_tag="PLP58_14570"
     CDS             complement(9370..11805)
                     /locus_tag="PLP58_14570"
                     /inference="COORDINATES: protein motif:HMM:NF020076.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VIT domain-containing protein"
                     /protein_id="HRJ09798.1"
                     /translation="MEAAHSPATPAPQKRRSRFLLVFGVLWPCFTLILEWSTRACASQ
                     YFDPLPTWTHLLLTGLVPLACWLARRNEDEGTTPAWQRAALGAAWVVASFYLFCFWGL
                     TLLAGFGVVFSIMLLATGGSLMFFLLPLATLGPFWGWVSLWKANRRLGPAPDASVWNR
                     QASAWGALAALLLLPAAEMPLWLTRTAAVRYEKAPAPEKAGWMSMLRTPGGEKILRGM
                     AYGGTQAAGSPGEWIMSGRTGVRGWDMAGLSLPSETAQALYYRVHGRLHSDLPPPKKL
                     SVLDMERGRWLPSELTESFDWDDQRGGDVVGARIRGLRMESSRMDWHLDTPTRLGYGE
                     WTLVFHNSRGDAQEARCQILLPPGGFVSRLTLWVNGEPREAAFSTQAKVKAAYREVVV
                     RQRRDPVLVNMTGPDRVMCQCFPVPANGDMQIRIGITAPLADAGLRMPVFLERNFAVP
                     DSSRHAVWVQSARDFTSSDPRPPVREAGQLTWRHDLSDNDLKDLVFRFPDDTPLPEAV
                     WCADPWPQQGRPGYFSGAWRTVTDSAPKSLIVVVDTSLALRPRAGDISRAISTLAEKA
                     PVTVLACTDSGWEEIPWEKSAQEWSGATFLGGRNNAPALEEALNRARSSGGAVVWLHG
                     PQPFEFEKHTGLEQIMERSVTPPVLHACALVHGPNRLLERLHRHRALQPSGRCADETE
                     LLSHMRRITGQTARRRLEIARQEAQPAGERVWDHLARLGVFQEVLAASQGPLPDPAPM
                     SLLAASYQLVTPLSGAVVLETEEQYKRAGLEPVDPNSTPQIPRIIVPEPSRLLLLFLA
                     CSLMLCRRRRHSV"
     gene            complement(11853..>13121)
                     /locus_tag="PLP58_14575"
     CDS             complement(11853..>13121)
                     /locus_tag="PLP58_14575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ09799.1"
                     /translation="RQVAVGWRVRNGTCRAGADMPGLSGTAYLAPGQMSTIIPVPVYR
                     DYVFEGEESFTVELTDSPQAGAIVDGASNTIVIVEDEDFFVPRPGAYCGLLDTEGWGA
                     LVKCTVTGEGRASGTVDFRGGTYRFTGGFDARGELVAAFARTSRPSIGLRLRFAEGWA
                     RCAASLRDPEGEWADGHIRYLPYSKASPAPQAGRHTHFCEGIGGGSVPVPVTGVSVVS
                     TDGSVRFVSRVADGQSATFASRIGQSDPDAGISGEAAFVAPLYGKTGNLYGTCDFGLG
                     PQQPGGETTLGWLKPWRPKDALYPAMPYQTIACQTVPFTPPPAGETVTQFTDGSVRFL
                     NSGTLLDGTSNTLLISEKNQVTITEPGNPLGCSIKIDAKTGSFSGKIKPPGSTKFINF
                     YGVMHQGAYNYGLGFFPGDQRAGEVVIADF"
BASE COUNT         2146 a         4220 c         4403 g         2352 t
ORIGIN      
        1 ccgattgccg cgcaccagaa gatgctcatc gaggagatca ggctgccgtc ggccgccgcc
       61 tcgtgcagca gcacctcccc cgccgaggtc agcgagagcg tccgcctccc gcgctcgatg
      121 agccgcgcgc ccagctcgtg ctcgatctcc tgcaccgcgc gcgagagcgc cggctgcgcg
      181 atgcgcaggc gcttcgacgc gcgcgagaaa ttcatctcct cggccaccgc ctggaagtaa
      241 cgcagatgtc gcaggtccat gcccgccttg tacacgccgg gaccgcgccc ggcaagccgc
      301 acgtcactcc tcccgccagt cttggtcccg cgctcaattc ccgcttcctg gcgcatcccc
      361 cctgacggcc cggcttattg cggcttgaaa atgaaataga ccgcgcccat caggcacagt
      421 cccgcccaga ggtaattcca ggtcagcggc gtcctcatgt aataaaccgc gaagggcacg
      481 aagaccgtca gcgtgatgac ttcctgcaaa attttcaact gcggcagtgt gaagaccgcg
      541 tgtccgatgc ggttcgccgg gacctggaag cagtactcga aaaacgcgat cccccagctc
      601 agcgcgatgg ccagcatcag aggtttcgag cgcaggtcgc gcagatgcgc gtaccaggcg
      661 aaggtcatga aaacatttga aagaagcagg agcaggatcg tcttggcggg gatcatgggc
      721 gcggggatgg cgcggaggat gccccggcgc gcgcaaaacg ccagcggaag cccgcgctcc
      781 ccctggatgt gccatgccgc ctttgcggac ggcgcatcct ggcgcactca agagactgca
      841 tcctgaattt tttggcggga atagcgtgga cagcgcgcgt ttgtttccag catcatcccg
      901 tcatgcgctg cggctttgtc attcggtctt cagcggatga cccgcctcgc gattgataga
      961 aaatgatcac cccgccttgc tccaccatga atccgcccgt ccagcaacgc cccggcctgg
     1021 gcatcattgg catgggcttg atgggcggcg ccctgatgag gatgaccggc gcggcacgcg
     1081 gctgggacat tgatcccgcg cgctgtgtcc atgcgggttc ggcggcggag gttttcgcga
     1141 actgcgccgt ggtcatgctt tgcctgcccg attcgcacat cgccagcgcc gtgcttgccg
     1201 ctgcggagac gcgtcccggc cagatcattg tggacaccac cacaggtgat cccggcgtga
     1261 tggccgccct gggcgcggcg ctcgcggaaa aaggctgcca ttatctcgat gccaccatct
     1321 cgggcagcag cgtccagctt ttgcggcgcg acgtgttggt gatggccggc ggggacgcgg
     1381 atgtggtgga gagatgccgg gaaattttcg acacgttcgc gagcgaggtg atccacgccg
     1441 gaccctgcgg cgcgggcgcg cggatgaagc tcgtcaccaa cctcgtgctc ggcctcaacc
     1501 gcgccgcgct ggccgagggg ctggcctttg ccgaagcctt gggcgtggaa cccgaatccg
     1561 ccctgcgggc aatgcgcgcg ggcaacgcct actcccgcgc catggacgtg aagggggaga
     1621 aaatgatccg gcgcgactac acgccccagg cccgcctctc gcagcatttg aaggatttgc
     1681 gtctcatgca gaactgcgcc ggggagcgcg gcatggacct gccgctggcg cggacgcatc
     1741 gcgccctgct tgaaaaagcg gagggcatgg gcctgggaag tctcgacaac agcgccatcc
     1801 tcgaagtcct gcgccttcac cgcgaatgaa tcccgcccct ctcaaccgcc gcgcgcgtca
     1861 tgccgtgctc gccgccgctt ttctcggcct tgtctttgac ggggtggagc tggggctgat
     1921 gccggtggcc tcgctttccg tctcgcaaag cctgctggga gaagcctaca cgcccacact
     1981 gggcggcgac tggtttgcgc gcttcaccgc cgcgctcatg cttggcgcgg ccatcggcgg
     2041 catcgcgctc ggcgctcttg gcgaccgcgt cggccgcacg cgggcgatgg gtgtcagcat
     2101 tctgttttat tctttgttcg ccgccatggg cagcctcgcg cggagccagg agcagatgct
     2161 gctcctgcgc ttcctcgtgg gcctcggtgt cggcggcatg tggccgaacg gcatcgcgct
     2221 cgtctcggaa tgctggccgc gcgcgtcccg gcccgtggtg tccggcgtga tgatggccgg
     2281 gctgaacgcg ggcatcctgc tgctttcgca actggcgcgg cactggccgg tgacgccgga
     2341 gtcatggcag tggattttca aactggcggg cgtgccggct ctcctgggca ttgtggtgct
     2401 gctggcactg ccggagtcgc cgcagtggct ggccgcgcgt gctgcgcgga aaacgcccgt
     2461 cacgccatta agggagcttt tccagccggg catggcgcgc gtcaccctgg cgggcatcct
     2521 catcagcgcc gtgcccatgg tcggagcctg ggcggccagc aaatggatga tcccctgggc
     2581 ggacaaggtg gccggcgggg cggacgctgg ttacaaggcg gccacgcagg gctggtgggc
     2641 gcttggcgct gtctgcggca gcttcatggg ggcgcagctt gccgcgcgga tggggcgccg
     2701 ccgtagctac ctgctcatca gcatctgcgc caccgcgctg accctcgcca tgttccagct
     2761 caccgcgccg ctgcggccgg gctttcatgc ggtggtctgc gcgcagggct ttgcagccac
     2821 tctgtttttc ggctggctgg ccctctatct gccggagctt ttccccaccc gcgtccgcgc
     2881 caccggcagc ggtttggcct acaactcggg ccgctttgcc acggctgccg gggtgctgct
     2941 ggcaggcttc ctgttttccg cgcttggcgg cgactacgcg cgtgtcggcg catggtgcgc
     3001 cttgatctac gccttcggaa tcatcgccat ctggttcgcg ccggacaccg gcaaaacacc
     3061 gcggccagaa tgaccttcat gcctccttgc gcttgagctg cgggaagagg atgacatccc
     3121 ggatgctttc ggcgccggtg agcatcatga tgaggcggtc aatcccaatg ccgatgccgc
     3181 cagcgggagg catgccgtgc tcaagggcga ggatgaactc ctcgtctatc ttctgtacct
     3241 cctcgcccgc ctggtgctcc aggcgctcgc gttggacgat ggggtcgttc agctcggagt
     3301 agccggggct gatttcctgg ccgttgatga cgagttcata aacatccacc acgctgtcat
     3361 cctgcgcgtt ttgcttggcc agcggcacga gctccttggg cacgtgggtg acgaagacgg
     3421 ggttgaagct cttttcctcg acgagctttt cgaagacttt ctggtccagc tcatagtgct
     3481 cgtctccggc atggatctcc tgcacgccga ggctctgcgc gcgcgccact ttggcctcct
     3541 tgtcgaggct gaaccagtcg tcgccagccg cgccgcgcac cagctcgcgg tagggcgcgc
     3601 gtttccaggg gcgggtgagg tcaatggtgc gagtcacgcc gccctcctcg tccttgtgct
     3661 cgatgacgag gccgccgcag aatttctccg ccagcgcgca gatcatgccc tcgacgaggt
     3721 ccgccatctg ctcgaagtcg gcataggccc agtaggcctc gagcatggtg aactcgggat
     3781 tgtggcggcg gctgaggccc tcgttgcgga agttgcggtt cagctcgaag acgcggttga
     3841 agccgccgac gagcagccgc ttcaaataca gctcgggggc gatgcgcagg tacatcttct
     3901 ggtcgagcgc gttgaagtgc gtctcgaagg ggcgcgcggc ggcgcctccg ggcacgtcct
     3961 gcatcattgg agtctcgacc tccaggaagt cccgcccgtg caggtaggcg cggatttcgg
     4021 cgatcatttt gctgcgcgtg acaaagagct ggccgctgcg ctcgttggag acgaggtcca
     4081 ggtagcgctg gcggtatttg atctccttgt cggtgacgcc gtgccatttg tccggcagcg
     4141 ggcgcagcgc cttggagagc ggggtcagcg cggccacatg cacggagcgc tcgccgcgct
     4201 tggtcacaaa ggtcctgccc tcgacgccga cgaagtcgcc gatgtccatt tgatgaatga
     4261 acagcgcgta ggcatcgtcg cccacgtcac ccttgctcaa gtagcactgg atgcggcctg
     4321 tgatgtcgcc cacgtcaaag aaggtggcct tgcccatctc gcgccgcgaa agcacacgcc
     4381 cggcgatcct cacctccacg ccctcggcga cgatgccctc agtgagctgg cgcttgagcg
     4441 tgccgggatc gttggtgact tcaaacctgc cgccgaacgc cgcgatgccg cgtttctgca
     4501 attcatcgag cttggctcgg cgaaattgga gcagttcgga ttcggtgtgt tcggtttctt
     4561 gcatggtctg tcgtcaggtg ggcaggctct ttagccgcat cccgcctgtt tgccagggca
     4621 aaaaagtcac cctcaaccct cctgatccgc gatgcggccg atgaggggat attgttcgat
     4681 ctcgaagacg ctgccgctca ccgggctgct ggcgctgctg acccaataga cggcggcctc
     4741 ggcgatgtcc tcgggcgaga gcaggcggcc aaagggcgct gccacaggcg gcagatggtc
     4801 cggccagtcg ggaggcaggc cgtcgccgac cttggtgtgg tattcgtttt cggtgagcgt
     4861 ccagccgacg ttcatctgat tgacgcgcac acggtcgcgg ccgagcgagt tggccagatt
     4921 ccgcgtcagg gtcatgagac cgccctttgt catggagtac acgggcaggt ttgactggcc
     4981 gcagtgcgcg aggatgctgc cgatgttcag cacgctgccc tggctttcct tcaaagccgg
     5041 cagcagcgct cggatgagca tgaagggcgc gcgcaggttc acagccacca tgcggtcaaa
     5101 gaagacgagg tcggtttccg tgatgcgcgc gcgggtggtg agcgcggcgt tgttcaccag
     5161 ggcgtcgagc cggccaaagg cggcgactgc ggcggctgcg agctcatccg ggtgggcggc
     5221 atctgcgaga tcacccgcga tgaaggccgc gcggtccgtg cccagctcct ggcgcagggc
     5281 ctcaccgcgc gcggtgtcgc gcccatgcac aatgacacgc gcgccttcct gcgcgaagcg
     5341 gcgcgccatg gccgcgccga tgcctgtcgt gctgccggtg atgaggatga tctggtcttg
     5401 gagtcgcatg agaaatgggg ggagacgggc cgggagagat agccggagcg gcggggcggg
     5461 caaatgattc gctgccttca aaatgtccgc cggcatttgt atttccacac gcccgcgcga
     5521 aagaagcctg atgacccgac tccgagccga cttgatgctg ctggcctgcg ccgccgtttg
     5581 gggcgtcgcg ttcatctgcc agaagcgggc catggcgcac atcggtcctt ttcttttcat
     5641 cgccctgcgc gcgtggctgg cctgcgcggc gctggcgcca ctggcctggc gggagtggaa
     5701 aaaggaaggc gcgcgtgtga acccgcgccg ttttctccgg ctaagtctgc tggcagggct
     5761 ggccttcttt ggcggcgcgg catttcagca ggcgggactg gtgacggcga cggtgacgaa
     5821 cgcgggcttc ctcactgcgc tttatgtggt catcactccc tttctggcct gggcattcat
     5881 gcgccaaaag ccggggccgc aggtgtgggc ggcggcggtt ttgtccttcg gcggcacctg
     5941 gttgcttggc ggcggttcca tcagcgcgct gtcatccggt gatgtgctgg tggcggtttc
     6001 ggccgtgctt tgggcgctgc atgtcgtgct cgtcgggctc gcggcgggcg gcggcatggc
     6061 ggcggcattc acgggcgcgc aatttgtcgt cgtgggcacc ctggccggac tggcggccgc
     6121 attcctggag gaggctggct tcgatgccat ttggcaagcc ggtcccgaca ttttgtatgt
     6181 gggcgtgctt tccagcgcgc tgaccttcac gctgctcgcc gtggctttgc gcgccacgag
     6241 gccatccgag gcggcggtca tcctctccac cgagtcgctg ttcgccgcgt tcggagctta
     6301 tgtcttgtta ggcgagcgtt tgaccacgac gggctggctg ggcgcggcgg ccatctttgc
     6361 cgccactttg ctcgtgcatg cgccggtgcg cgccacacgg cgcacgcagc cgcccggatg
     6421 actcaccctg gcagacccgc cagccggcgc gcggtttcca gagtgtctat ctcgccgggt
     6481 gtcttgtccc ggcggatggc tttgatgcgc gggaaacgca gggccaggcc gctgtcatgg
     6541 cgcttgctgg gctggatgga gtcgaaggcg atctccagca ccgtgtccgg cagcacggtg
     6601 cggactcggc ctttttgctc gagtgtgttc gaggtgaaat gcgcggtcag ttcctcaatc
     6661 tcggcatcgg tgaggccgga gtaagccttc ccaatcacgc gcaaagcgcc cgcctcgtca
     6721 cggacggcaa aggtgtagtc acttaaaaca tggctgcgct tgccatgtcc ctgctcggct
     6781 ttgaccacca ccacatccag cgtggcaaag gccttcttca gtttcagcca ggatttgccg
     6841 cgccggcccg ggcggtaggc cgcggcgggg tctttggcga tgaggccctc attcccgcgg
     6901 cggcgcgcgg catgaaaagc agcctcaatg tcgaatggcg aggccgcctg ccagatctca
     6961 atgacgccca tcggctccgg caaagccacc gcctcaagtt ccgcgcggcg ctcgctcagc
     7021 gggcgctcca gcaggctcgc gccgttccgc cacagcacat caaagaccac atagcgcacg
     7081 cggatgtcgc tgggcaggaa aaggtccgcc tgatcccggc gtcccagccg tttttgcaga
     7141 tcgaaaaacg agagcttttt gtcctcggca taggcgatga tctcgccatc caggatgacc
     7201 tcgtcgcgca tcatccgcgc ggcatccagg atctcgggaa attgaaaacc caccggcttc
     7261 aaatcccgcg tgtaaatctc cgcgcggttc gcgccccggt gcaactgcgc gcggatgccg
     7321 tcgaacttgt cctccagcca cacggtgggg gaagttcgaa attcgaagtt cgaagtttga
     7381 agcacgggaa acggcgcaat ggcggagact gtggactctt cggttttcga atttcgagct
     7441 tcgaatttcg aacctcgatc ctcccccagt ctctcccaaa tgtcctccgc cgtctcctcg
     7501 ggactcgcca gcatcacttt gaccggcaca ccgagcctcg gcgcggcctc gtgcagccgg
     7561 cctgcttgag cgagttgcgc ggcgcggccg atgtcgccgc agagcatggc ggcctcgcgc
     7621 acgctgtcgg catcctgatc aaatgcctgc gcgatcgctt cctcgatgag tccctcgcgc
     7681 gatcccatgc gcaactctcc ggtcatgagc cgcacgagcc aggaacccgt ctccgcatcc
     7741 gaagcggcca ggcactcgcg cagcaggcgc gtcttcagca ccgggccgcg cgcctctcgc
     7801 aggtgtcgaa aaaaagcgtc cgttccggtc agtgtcagcg gggtgttcat cggtgcctga
     7861 gcgcccctca tgccgagcat gagaaaagcc gtgcgccccg cgtccgcctg cgagcgggag
     7921 atgacgcgat actccgcctc gctgatgcca gccagctcct gcaaggcgcg ccggaacacc
     7981 gcccagcccg tgttcagcgg agcctcgcca gcggcggggt caaagaccag ccccgagcaa
     8041 tacctggccg ccagagccag ttcgtccggc ggcaaagagc gcaggtattc cgcgagcagc
     8101 gcggtctttt tcagtcgcga agcctccgcc gcggtgcgcg cgcaagtgag cgcccaggct
     8161 gcgaaatccg aggggtgaaa tttgctggac ggctcagcat ccggcgcggc atggagaaca
     8221 ggcaggacgc ctgttcccca tgaacttcga tcctcgaact tcagaaactc caactggtcc
     8281 tccttttcga gagtccaagc ctcccatcca cgcgcgcgca aatcggcggc gaactcccgc
     8341 gtggagccat gcaccagcca cacgcgcttt ggattcaccg tctccaccgt ttgcaaaagc
     8401 tcgtcatagt ccgcgtgatc actgagtgga aaagcctcgt ccacttggta acgataccgc
     8461 gctcccggct gcatggccca gccgctgagc atcgccgtgc ggcagacctt gagcttgcgc
     8521 aaaggctggc tgcggttcgc gctgggagga aagacgagca catgcccgct agcttcggcg
     8581 gcatcaaaga ggcgatgctc aggcagcgcg ccaatgacgg gcgccacggc ctgtgtcatc
     8641 tccagcacgg acttgtgcag catcacgggg taacccgaac ccgccaggat gcgcaggatt
     8701 tcctgggcct tgcccaatga gtatccgagc agaacgggaa tgccgccatc ctccagcgtc
     8761 tcgcgcaccc agcgcagcac ggcggcctcc gtttccgcgt gcggcgggaa gcggaactgt
     8821 ggcagaccaa aggtgcattc catgaccagc acatccgcgc gcggcagctc gcagggttcg
     8881 gcgctcaggc cgtggcgcag cttgtaatcc cccgtgtaga gcagcgtggc gccatcgctg
     8941 aggcgcgtga gatgaagcat ggcagatccc acgatgtgcc ccgccgggta aagccgcagc
     9001 cgccagccat ggatctccag gtcctgccgc agcggcagca tctgaaattc cgcggtctcc
     9061 ttctccccat accgggcgcg catgatgtgg cgtgtgacct ccgagcaaaa cgtcagccgg
     9121 tgccgcgcga agtggtcgct gtgcgcatga gaaataaatg cccgctccac gccaaaatgc
     9181 gggtccagcc acaaatccgc ctcgggcaga taaatgccgc ggcggtgggt gacttcgatc
     9241 agagggggca tggtttaaga aatccgagca tacgcgcggc gagggcggga gggcagagca
     9301 ttccaggctt gagagacatc gtccgacctt cctgggtgaa tctgatgcgc catgcatgaa
     9361 caagtcttgc tacacagagt ggcggcggcg tctgcacagc atgagggagc aggcgaggaa
     9421 aagcagcagc agccgcgagg gttccgggac gatgatccgg gggatttgcg gtgtggagtt
     9481 tgggtccacg ggttcgaggc cggcgcgctt gtactgctcc tccgtctcca gcaccacggc
     9541 acccgagagc ggggtcacaa gctgatacga agcggccagc agggacatgg gcgcggggtc
     9601 gggaaggggg ccctgcgagg ccgcgagaac ttcttgaaag acaccgagcc gcgccagatg
     9661 atcccatacg cgctctcccg caggctgcgc ttcctggcgg gcgatctcaa gcctgcgccg
     9721 cgctgtctgc cctgtgatcc ggcgcatgtg ggagagaagc tccgtttcat cggcgcagcg
     9781 gcctgaaggt tgcaaggcac ggtgcctgtg caggcgctcc agcaggcggt tggggccgtg
     9841 aaccagggcg caggcgtgca ggacgggcgg cgtgacggag cgctccatga tttgctcgag
     9901 tccggtgtgt ttttcgaact cgaagggctg cggcccgtgc aaccagacga ctgcgccgcc
     9961 gctgctcctg gcgcggttga gtgcctcctc cagagcgggg gcgttgttgc gtccgccgag
    10021 aaaggtcgcg ccgctccatt cctgggcgga tttctcccaa gggatttcct cccagcccga
    10081 gtccgtgcag gccagcacgg tgaccggcgc tttctccgca agggtggaga tggctctgct
    10141 gatgtcaccg gcgcgcgggc gcagggctag cgatgtgtcc acgacaacga tgaggctttt
    10201 cggcgcggag tcagtcacag tccgccatgc gcccgagaaa taaccgggcc tgccctgctg
    10261 aggccaggga tccgcgcacc acactgcctc cggcaatgga gtgtcgtcgg ggaatcggaa
    10321 gaccaggtct tttagatcgt tgtcagagag gtcgtgacgc caggtgagct ggccggcctc
    10381 gcgcacggga gggcgcggat cactgctggt gaagtcgcgc gcgctttgca cccagactgc
    10441 gtggcgggag gagtccggca cggcgaagtt tctttcgaga aacacgggca tgcgcagtcc
    10501 cgcatccgcc agcggcgcgg tgatgccgat gcggatttgc atgtcgccat tcgcaggcac
    10561 cggaaagcac tggcacatga cccggtcggg gccggtcatg ttgaccaata cagggtcgcg
    10621 cctctggcgg acaaccacct cccggtatgc ggctttgact ttggcctggg tggaaaatgc
    10681 ggcttcgcgc ggctctccgt tcacccagag cgtgaggcgg gagacgaatc cgcccggcgg
    10741 cagcaggatc tggcagcgcg cctcctgcgc gtcaccacgg ctgttgtgaa agacgagggt
    10801 ccactcgcca tagcccaggc gtgtgggcgt gtcgagatgc cagtccatgc gggagctctc
    10861 catgcgcagt ccgcggatgc gcgcgcccac cacatcaccg ccgcgctggt catcccagtc
    10921 gaatgactcg gtgagttccg agggcagcca gcggcctcgt tccatgtcga gcacggagag
    10981 ttttttcggc ggcggcaggt cactgtgaag ccggccgtgg acgcggtaat agagcgcctg
    11041 cgcggtttcg ctgggaaggg acagtcccgc catgtcccag cccctgactc ccgtgcggcc
    11101 gctcatgatc cactcgcccg ggctgcctgc ggcttgagtg ccgccgtagg ccattccgcg
    11161 caggattttc tcgccgccgg gagtgcgcag catgctcatc cagccggctt tttccggggc
    11221 tggcgccttt tcataacgca cagcggcggt gcgcgtgagc cagagcggca tctcagcggc
    11281 gggcagcagc aggagcgcgg ccagagcgcc ccaggccgaa gcttggcggt tccagacaga
    11341 ggcatccggc gcgggtccca gacgccggtt tgccttccac agagacaccc agccccagaa
    11401 tggtccgagt gtggcgagcg gcagcaggaa aaacatgagg ctgcccccgg tggccagcag
    11461 catgatcgaa aagacaacgc cgaaccccgc cagcagcgtc agcccccaaa agcagaacaa
    11521 ataaaagctc gccaccaccc aggccgcgcc aagcgccgcg cgctgccagg cgggagtcgt
    11581 tccctcatcc tcgttcctgc gcgccagcca gcacgcaagg ggcaccaagc ccgtcagcag
    11641 cagatgcgtc caggtcggca gcggatcaaa atactgcgag gcgcacgccc tcgtgctcca
    11701 ctccaggatg agtgtgaagc agggccagag cacgccgaag acgagcagaa aacgcgagcg
    11761 ccgcttttga ggagcgggtg tggctgggga atgggcggcc tccatgccgc gatgctaacg
    11821 cacacgctgt cagcgggtca atttttccag catcaaaaat ccgcgatgac cacctcgccg
    11881 gcgcgctggt cgccggggaa gaagccgagg ccgtagttgt aggcgccctg gtgcatgacg
    11941 ccgtaaaagt tgatgaactt cgtcgagccg ggcggtttga tcttgccgct gaaagagccg
    12001 gttttcgcgt caatcttgat ggagcagccg agagggttgc cgggctcggt gatggtgact
    12061 tggttcttct cggagatgag cagggtgttg ctggtgccgt ccagcagagt gccgctgttg
    12121 aggaagcgca cgctgccatc tgtgaactgg gtcacggttt ctccggcggg cggcggggtg
    12181 aagggcacgg tctggcaggc gatggtctgg tagggcatgg cggggtagag cgcgtccttg
    12241 ggccgccagg gcttcagcca gccgagcgtg gtctcaccgc cgggctgctg ggggccgagg
    12301 ccgaaatcgc aggtgccgta gaggttcccg gttttgccat agagcggcgc gacgaaggcc
    12361 gcctcgccgc tgatcccggc gtcggggtcg gactggccga tgcggctggc aaaggtggcg
    12421 ctctggccgt cggcgacccg gctcacaaaa cgcacgctgc cgtccgtgct gacgacgctg
    12481 acgccggtga cgggcaccgg cacggagccg ccgccgatgc cctcgcagaa gtgcgtgtgg
    12541 cgcccggcct gcggcgcggg gctggccttg gagtagggca ggtagcggat gtggccgtcg
    12601 gcccattcac cctcgggatc ccgcagggag gcggcgcagc gcgcccagcc ttcggcaaag
    12661 cgcaggcgca ggccgatgga gggccggctg gtgcgggcaa aggcggccac cagctccccc
    12721 ctggcgtcaa agccgccggt gaagcggtag gtgccgccgc ggaagtccac cgtgccgctg
    12781 gcccggccct cgccggtgac ggtgcatttg accaatgcgc cccagccctc cgtgtcgagc
    12841 aggccgcaat aggcgcctgg ccggggcaca aaaaagtcct cgtcctccac aatgacgatc
    12901 gtgttgctgg cgccgtccac gatggctccg gcctgggggc tgtccgtcag ctcgacggtg
    12961 aaggattcct ctccttcaaa aacataatcg cggtaaaccg gcaccgggat gatggtgctc
    13021 atctgtccgg gagccaggta ggcggtgccg gagaggccgg gcatgtccgc gccggcgcgg
    13081 caggtgccgt tccgcacgcg ccagcccacg gccacctggc g
//
LOCUS       DAOMYJ010000146        13104 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Prosthecobacter sp. isolate AMR_MDS_5629
            NODE_1588_length_13104_cov_21.438041, whole genome shotgun
            sequence.
ACCESSION   DAOMYJ010000146 DAOMYJ010000000
VERSION     DAOMYJ010000146.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867850
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prosthecobacter sp. (wastewater metagenome)
  ORGANISM  Prosthecobacter sp.
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Verrucomicrobiaceae; Prosthecobacter.
REFERENCE   1  (bases 1 to 13104)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13104)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.30
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:34:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,742
            CDSs (total)                      :: 4,684
            Genes (coding)                    :: 4,676
            CDSs (with protein)               :: 4,676
            Genes (RNA)                       :: 58
            tRNAs                             :: 55
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13104
                     /organism="Prosthecobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1588_length_13104_cov_21.438041"
                     /isolate="AMR_MDS_5629"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1965333"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1213)
                     /locus_tag="PLP58_14580"
     CDS             complement(<1..1213)
                     /locus_tag="PLP58_14580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009965680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HRJ09800.1"
                     /translation="MTNPASVLIVDDEKHTRDGLRLSLEDDFDCYVAADAAEALEHLR
                     HDQIDVMLTDLRLGQDDGMKLLEEALALPQPPVCIMMTAYGSVDTAVEAMRRGAYHFV
                     TKPLNLDEVELLIKRALRSRHLEKENTALKQQVGQKFSIEGIIGKSDALKPVLETIQQ
                     VAPTRATVLIEGESGTGKELAARAIHHLSGRPAAKLVTVHCAALSPQILESELFGHEK
                     GAFTGAQERRIGRFELADGGTLFLDEIGEIDASTQVKLLRALGEQTIERVGSAKPVKI
                     NVRVIAATNRDLGKMVEEGKFREDLYWRLRVVTLQMPPLRARKGDIPVLAEHFLKELS
                     AANGKPFKPLGEDALPALMSYDWPGNVRELRAAIEHGVVMSNTTRVMAKHLPAYLSQP
                     GGLGWRVPPASQ"
     gene            1307..2668
                     /locus_tag="PLP58_14585"
     CDS             1307..2668
                     /locus_tag="PLP58_14585"
                     /inference="COORDINATES: protein motif:HMM:NF019040.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1501 domain-containing protein"
                     /protein_id="HRJ09801.1"
                     /translation="MLTLSGKSHGILCDGMARRDFLRIGGLAMGGLTLPDLLKAEAEA
                     RTGRSYKSIIMIYLCGAPPHQDMWDLKMDAPLEIRGPYKPIPTNVPGIQISEHAPRLA
                     KMMDKLVPIRSMWGSPTGAHDSFICYTGRAPNPAGGAAPTGAQSDHPSFGSVISSLQG
                     QADPAIPAFVGLAPKAGHPPYGSPGHPGYMGPAHSAFRPNGAGVEDLKLNNVSVARLD
                     DRRSLLEGFDQFRRDVDHSGLVESMDSFNQQAFNVLTSSKLLDALDLEKESPKTRERY
                     GQGDPKNYGDGAPLNLEHFLMARRLVEAGARVVTLNFGRWDFHDNNYPQLLRHLPLFD
                     QGMSALVQDLHDRGLDKDVAVVAWGEFGRTPTLNAQGGRDHWPRVGGALLAGGGFKTG
                     QVIGATDRLAGEPTERPVHFGEVFASLYQFMGIDPHKTTVNDLTGRPQYLVDGWQAMP
                     ELI"
     gene            2675..4120
                     /locus_tag="PLP58_14590"
     CDS             2675..4120
                     /locus_tag="PLP58_14590"
                     /inference="COORDINATES: protein motif:HMM:NF019040.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1501 domain-containing protein"
                     /protein_id="HRJ09802.1"
                     /translation="MPLPHPAHPLSRREILQAGAVGLLGLGMNQLTSLRALAAPGSTP
                     QPRAKSVIYIFLSGGLAQHESFDMKPDAPMEVRGEFKPIRTRTPGTHICEHLPRLARI
                     SEKWSLVRSLTHGNSEHSQGHHVMLTGRSDVPPDFDPTKPKKSDHPSMAALASALLPR
                     RKDNLPPSIVLPDKIVHRTGRTLAGQFAGTLGSQHDPWFLEMAPYHPNHYGAYPGYLF
                     HHERGAETDDTLTFRAPHLSLPQGLSLDRVLDRVSLRDSLETQTENLAALAGDPAFDT
                     YREAAVSLLGNRQVHDAFSLDKVPPEKLDRYGRHSFGWSLLMARRLIESGVRMVQVNL
                     GNNESWDTHQAAWRNLKDYLLPPMDQAVSALIEDLDASGLLDDTLIVMGSEFGRTPKI
                     SKITATALPGRDHWGHVQSVLLAGGGIQGGRVIGSSDKIGGYPASDAQIPENLAATIY
                     EALGLPREIMWHDFTGRPHPLYMGSPIAGLV"
     gene            4181..5524
                     /locus_tag="PLP58_14595"
     CDS             4181..5524
                     /locus_tag="PLP58_14595"
                     /inference="COORDINATES: protein motif:HMM:NF019040.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1501 domain-containing protein"
                     /protein_id="HRJ09803.1"
                     /translation="MLSIPGSSHRGFCDGKTRRDFLKIGGLAMGGLSLPELLRAEASQ
                     NQKPGHKAVIMVFLPGGPSHQDILDLKPHAPSEIRGEFKPIGTNVDGIQICEHLPRLA
                     RMMDRCTIIRSMADCDSSHDAFQCLTGRSARQQPPGGWPAFGSIVSRLQGTVDKAMPP
                     FVGLSPKMGHMPWARNGEPGYLGVAHAPFEPNRGGGTDDMTLRRMTLDRLHDRAGLLK
                     SFDQLRRDLDASGLMEGMDAFNEQALGVLTSPKLLQALDLTREDSRIAERYGKGENKN
                     RDDGGPRLTDHFLAARRLVEAGTRVVTLGFSRWDYHSNNFGQLREDLPLLDSALSALL
                     TDLRERGMDKDVAVVVWGEFGRTPVINAQGGRDHWPRVSCAMLAGGAFRHGQVIGATD
                     KVAGEATERPVAFGEVFATLYHHLGIDVGKVTLDDFSGRPQYLIEGAHQPLAELV"
     gene            5602..6540
                     /gene="deoC"
                     /locus_tag="PLP58_14600"
     CDS             5602..6540
                     /gene="deoC"
                     /locus_tag="PLP58_14600"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007416619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004139 - deoxyribose-phosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0009264 - deoxyribonucleotide catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HRJ09804.1"
                     /translation="MPAAKAPASFRRLFDEVGTVDHVGIEERVAKYTTRSIKKKSKVF
                     GLRLAVTMVDLTTLEGKDTPGKVASLCQKALTPHDEGDIPPTAAVCVYPAMVRHAKKH
                     VGGTKVKVASVATAFPSGQAPLKTRLAEVRAAVNDGADEIDMVINRGAFLSGEFGLMQ
                     DEIAAVVEACGETTLKVILEVSELETYDNIRAASFLAMRAIRPGDFIKTSTGKTSSNA
                     TLGNNQVMLEAIRDHFLATGVPIAMKPAGGIRTAKQALHFLIAVKETLGDEWLNNRRY
                     RFGASALLNDLVRQLEKERTGAYQAPWNFSEATEGY"
     gene            6553..8046
                     /locus_tag="PLP58_14605"
     CDS             6553..8046
                     /locus_tag="PLP58_14605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007416618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HRJ09805.1"
                     /translation="MPKTLSPSKPKTNPARELVFGDLWEFDPAPETADPKLKKKYDIF
                     INGRFVPSRSGKYFATINPATEVKIADIALADASDVDAACSAAQRAFITWGRLPGKER
                     GKYLYRIARLLQDHAREFAVAETMDGGKPIKESRDFDVPMAAAHFFYHAGWADKLEFL
                     APGRQLAPHGVVGQVIPWNFPLLMLAWKIAPALAAGNCVVLKPAETTSITALKFGEIC
                     QEAGLPNGVVNIVSGAGETGAAVINHPLTSKVAFTGSTEVGKIIMRSLAGTDKKLTLE
                     LGGKAANIVFDDAPLDQAVEGIVNGIFFNQGHVCCAGSRLLVQESIADELLAKLKRRI
                     ATLRVGDPLDKNTDLGAINSAEQLAKISDLVKSGVEEGAEKHDNGCKLPAKGFFFRPT
                     IFANVSMSHRIAREEIFGPVLSILTFRTLEEAVEKANNTAYGLSAGVWTDKGSRILKM
                     STLLKAGVVWANTYNKFDPTSPFGGFKESGFGREGGRQGLLDYLTVH"
     gene            8060..8458
                     /locus_tag="PLP58_14610"
     CDS             8060..8458
                     /locus_tag="PLP58_14610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007417346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GxxExxY protein"
                     /protein_id="HRJ09806.1"
                     /translation="MNTNTHECFSDELLHGKLVYSIVGAAFEVLKGLRHGLHEKPYEN
                     AMVVELRLRGHALDQQRRFEVAYKGEHVGEFVPDLIVDGAVVVDAKVIDRITNLERGQ
                     MLNYLRITGLRVGLIINFHKPKLEWERVIL"
     gene            8488..9354
                     /locus_tag="PLP58_14615"
     CDS             8488..9354
                     /locus_tag="PLP58_14615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007417977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HRJ09807.1"
                     /translation="MSRLHITKTPKCYVGGAFIRSESGRVTELHDASGAFFANIPQCT
                     RKDLRNAVEAAAKAGPGWAKRSAFNRGQILYRLAEMMEARGVELADSITLSGVSKAEA
                     SREVAAAIERVVHYAGWTDKYEQVLGSVNPVASAHFNFTVTEPMGIVGILAPDEAPVL
                     GLLSLILPAITSGNSVVALASTTQPYPSILLGEMLATSDLPGGVVNLLTGVRSELIPT
                     FATHEHLRALSGVANADERKALRLGAAESVKRVHLRKAEEPIDWFADKMQGAEEIRAL
                     VEFKTTWHPVGG"
     gene            complement(9392..10465)
                     /locus_tag="PLP58_14620"
     CDS             complement(9392..10465)
                     /locus_tag="PLP58_14620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009963216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HRJ09808.1"
                     /translation="MTETTTAIHKFSCPACGAEAVWTPSKQALVCPYCGTESPAELKA
                     DGSLVEESDLAAALRAIPDDERGWKAERKSVRCQSCQAISVFDAAHVAKNCDFCGSPA
                     LLPLNDTGAPIRPGSLLPFKVSQSQVRENIRRWYGSHFWARRDLKDKALTDTLHGLYL
                     PYWTFDAHADCPWQAEAGYHYYTRDSQGRQQRRTRWESASGRVKHSFDDVLVPASKGV
                     HSKLLKGLEPFPTTTDLVPYDAGYLSGWVVEQYQLDLIQAAKHSRERMDDELRSMCAA
                     RVPGDTHRNLRISPAYTGQTFKHVLLPVWLLSYTYGKKNYQVTVNGATGRITGEYPLS
                     WVKIAIAVILALLVVVLIAQFSK"
     gene            10601..11707
                     /locus_tag="PLP58_14625"
     CDS             10601..11707
                     /locus_tag="PLP58_14625"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HRJ09809.1"
                     /translation="MRIQRIPSLLLPVFISFPLLAAAAGLRAAAPHVTGGKPLPPLPE
                     GAFTLVVIPDTQSYAGKGCKATPDSTDSVTNSNLEAQVQWITANREAQNIVFVTHVGD
                     IVDQNNDEQWSVAKRHLDQLRGVAPFALTVGNHDMTSNGDARLFQRHFPAASFADQPW
                     YLESYRHDRRDQHVSADNVNSAQVFSAGGLDFLHMSLECNAPDDVLAWADAVLRRHPE
                     RLAIITTHMDLGIENKPATTEGYIHDPKGRMRWTKIHGERGNSGAWMWGKLYRKHANL
                     RFILCGDQSRVTALRREDRADDGHVIHSLLSDYMSQPVLRLMRFLPAEQRVDVLTYEV
                     GRGFLVESALHVPETGQHQFSLHLPLRPGRAPSS"
     gene            complement(11752..12735)
                     /locus_tag="PLP58_14630"
     CDS             complement(11752..12735)
                     /locus_tag="PLP58_14630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009965405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1080 domain-containing protein"
                     /protein_id="HRJ09810.1"
                     /translation="MKITHLLAALFLTSSAIAADNPYLGRWALELPGGRAGWLGVEEK
                     DGALTASVLWGGGSVVPAQGAEIKDGKLLVTRLSNAKRKNKAGGEINEKVTETLTVQV
                     SGDALTGATVKAKDDGKEFGQGEFTGKRIPALPPRPDLAKIKFGMPVSLFNGSDLSGW
                     RLVNEADLNGWKVEDGVLMNRVVKEPGKHFGNLRTGAEFEDFNLTLEVRTQEASNSGI
                     YLRGIYEVQVMESFGKPVDSHHMGALYSRITPSVAAEKPVGEWQTLDITLVDRHLTVI
                     LNGTTIIDNQPVLGCTGGALTSDESKPGPIFLQGDHTNVDYRNMLLRPVLK"
     gene            12874..>13104
                     /locus_tag="PLP58_14635"
     CDS             12874..>13104
                     /locus_tag="PLP58_14635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ09811.1"
                     /translation="MTESEATPDNPAQPSAAQVDLSQLRMMPAWVAGMSSLANPAKYE
                     EREEREERRGQRPGGRDRRDGPPPRRDGPPPRR"
BASE COUNT         2411 a         4290 c         4076 g         2327 t
ORIGIN      
        1 gctgcgaggc gggcggcacg cgccagccga gcccgccggg ttgggagagg taggcgggca
       61 ggtgttttgc catgacgcgg gtggtgttgc tcatgacgac gccgtgctcg atggcggcgc
      121 gcagctcgcg cacgttgccg ggccagtcgt agctcatgag cgcgggcagg gcgtcctcgc
      181 cgaggggctt gaagggtttg ccgttggcgg cggacagttc tttgagaaaa tgctcggcca
      241 gcacggggat gtcgcctttg cgcgcgcgca gcggcggcat ttggagggtg acgacgcgca
      301 gccgccagta gaggtcctcg cggaatttgc cctcttcgac cattttgccg aggtcgcggt
      361 tggtggcggc gatgacgcgc acgttgatct tgaccggctt ggcgctgccg acgcgctcga
      421 tggtctgctc gccgagggcg cgcagcagct tcacctgcgt ggaggcgtct atctcgccga
      481 tttcgtcgag gaaaagcgtg ccgccgtccg ccagctcgaa gcgcccgatg cgccgctcct
      541 gcgcgccggt gaaggcgccc ttctcatggc cgaagagctc gctttccaaa atctgcggcg
      601 agagcgccgc gcaatgcacg gtgacgagct tggcggcggg cctgccgctg agatggtgga
      661 tggcgcgggc cgcgagctcc ttgccggtgc cgctttcccc ctcgatgagc acggtggcgc
      721 gtgtgggcgc cacctgctgg atggtttcga ggacgggctt gagcgcgtcg gatttgccga
      781 tgatgccctc gatggagaat ttctgcccga cctgctgctt gagcgcggtg ttttccttct
      841 ccagatggcg gctgcgcagg gcgcgtttga tgagcagctc cacctcgtcg agattcagcg
      901 gcttggtgac gaaatggtag gccccgcgcc gcatcgcctc caccgccgtg tccacgctgc
      961 cgtaggcggt catcatgatg cagacgggcg gctgaggcag agcaagcgcc tcctccagca
     1021 gcttcatgcc gtcgtcctgg ccgagtcgga gatcggtgag catcacgtct atctggtcat
     1081 ggcgcagatg ctccagcgcc tcggccgcgt ccgcggccac ataacagtcg aagtcatcct
     1141 ccagtgacaa ccgaagcccg tcacgggtgt gtttttcatc atccacaatg aggacagagg
     1201 cgggattggt catggtgctg ccgcatcttg gacaaggcgc tgaaaaagtc aaaacgtctc
     1261 acttttccat ctgaaagcct gcctgtgtca gccgcgtatg tggcccgtgc tcactctctc
     1321 cggcaaatcc cacggcatac tctgcgacgg catggcccgt cgtgatttcc tgcgcatcgg
     1381 cggcctcgcc atgggcggcc tcacgctgcc tgacctcctc aaagccgagg ccgaggcaag
     1441 gacaggccgc agctacaagt ccatcatcat gatctacctt tgcggcgcgc cgccgcacca
     1501 ggacatgtgg gatctgaaaa tggacgcgcc gctggaaatc cgcggcccct acaagcccat
     1561 ccccacgaac gtgcccggca tccaaatctc cgagcacgcg ccgcgcctgg cgaagatgat
     1621 ggacaagctg gtgcccatcc gcagcatgtg gggctcaccc accggcgcgc acgactcctt
     1681 catctgctac accggccgcg cgccgaaccc cgccggcgga gccgcgccca ctggcgcgca
     1741 gtcggaccat ccttccttcg gctctgtcat ctcctctttg caaggccagg ccgaccccgc
     1801 catccccgcc tttgtcggac tcgcgcccaa ggccggccac ccgccttacg gctcgccggg
     1861 acatccaggt tacatggggc ccgcgcattc cgccttccgt ccgaacggcg cgggagtgga
     1921 ggatttgaag ctgaacaatg tctccgtcgc ccgcctggac gaccgccgct cgctgctcga
     1981 aggtttcgat caattccgcc gcgatgtgga ccacagcggc ctggtggaga gcatggactc
     2041 tttcaatcaa caagccttca acgtgctgac ctccagcaaa ctgctcgacg ccctcgacct
     2101 ggaaaaggag tcgccaaaaa cccgcgaacg ctacggccag ggcgacccga aaaactacgg
     2161 cgacggcgcg ccgctgaacc tggagcactt cctcatggcc cgccgcctcg tcgaagctgg
     2221 cgcgcgcgtg gtgacgctga acttcgggcg ctgggacttc cacgacaaca actacccgca
     2281 actgctgcgc cacctgccct tgttcgatca aggcatgagc gcgctggtgc aggacctgca
     2341 tgatcgcggt ctcgacaagg acgtggcggt ggtggcctgg ggcgagtttg gccgcacccc
     2401 caccctcaac gcccagggcg ggcgcgatca ctggccgcgc gtgggcggcg ccttgctggc
     2461 cggcggcggt ttcaaaaccg gccaggtcat cggcgccacc gaccgcctcg cgggcgagcc
     2521 caccgagcgc cccgtgcatt tcggcgaggt cttcgccagc ctctaccagt tcatgggcat
     2581 tgacccgcac aaaaccaccg tcaacgacct caccggccgc ccgcaatacc tcgtggacgg
     2641 ctggcaggcc atgccggagc tgatttgaaa atccatgccc ctcccgcacc cagcgcatcc
     2701 gctcagccgc cgtgagattc tccaggcggg ggcggtgggt ctcctggggc tggggatgaa
     2761 tcaactcacc agcctgcgcg ccctggccgc tcccggttcc acgccgcagc cgcgcgccaa
     2821 gtctgtcatc tacatcttcc tctccggcgg attggcgcag catgagagct tcgacatgaa
     2881 gcccgacgcg cccatggaag tgcgcggcga gttcaagccc atccgcaccc gcacgcccgg
     2941 cacccacatc tgcgagcatc tgccgagact ggcgcgtatt tcggaaaaat ggtcgctggt
     3001 ccgctcgctc acccacggga acagcgagca ctcccagggc catcacgtca tgctcaccgg
     3061 acgcagcgat gtgccgccgg actttgaccc gaccaagccg aaaaagtccg accaccccag
     3121 catggccgcc ctcgccagcg ccctgctgcc gcgccgcaag gacaacctgc cgccctccat
     3181 cgtcctgcct gacaaaatcg tccaccgcac cggacgcact ctggcgggcc agttcgccgg
     3241 cacgctcggc tcccagcacg atccctggtt tctggagatg gcgccctacc atcccaacca
     3301 ctacggcgcc tatcccggct acctcttcca tcacgagcgc ggcgccgaga ccgacgacac
     3361 actcaccttc cgcgctccgc atctttccct gccgcagggc ctctcgctgg accgcgtgct
     3421 cgaccgcgtc tcgctgcgcg actctctgga aacgcagaca gaaaaccttg cagccctcgc
     3481 aggcgatccc gcttttgaca cctaccgcga agccgccgtc tccctgctcg gcaaccgcca
     3541 ggtccacgat gccttcagcc tcgacaaagt gccgcccgaa aagctggacc gttacggcag
     3601 gcacagcttc ggctggtccc tgctcatggc gaggcggctc atcgaaagcg gcgtgcgcat
     3661 ggtgcaggtg aacctcggca acaacgaaag ctgggacacc caccaggccg cctggcgcaa
     3721 cctcaaagac tacctcctgc cgcccatgga ccaggcagtc agcgcgctca tcgaggacct
     3781 cgacgccagc ggcctgctgg atgacacgct catcgtcatg ggcagcgagt ttggccgcac
     3841 gccaaagatt tccaaaatca ccgccaccgc cctgcccgga cgggaccact ggggccatgt
     3901 gcaaagcgtg ctcctcgccg gtggcggcat ccaaggcggg cgcgtcatcg gcagcagcga
     3961 caagatcggc ggctaccccg ccagcgacgc gcagattcct gaaaatctcg ccgccaccat
     4021 ctatgaagcc ctcggcctgc cgcgcgagat catgtggcac gacttcaccg gccgcccgca
     4081 cccgctctac atgggctcgc ccattgctgg gctggtgtga ttttccactc cggcaagacg
     4141 ccggccgggc accgcgtatt ctgccaacca cttcccggcc atgctctcca tcccaggctc
     4201 ctcccatcgc ggtttttgcg acggcaaaac gcggcgcgat ttcctcaaga tcggcggtct
     4261 cgccatgggc gggctttccc tgccggagct gctgcgcgcc gaggcctctc aaaatcaaaa
     4321 gcccgggcac aaggccgtca tcatggtctt cctgcccggc ggcccctcgc accaggacat
     4381 cctcgatttg aagccgcacg cgccctcgga gattcgcggc gagttcaagc ccataggcac
     4441 caatgtggac ggcattcaaa tatgcgagca ccttccgcgt ctggcgcgca tgatggaccg
     4501 ctgcaccatc atccgctcca tggccgattg cgacagcagc catgacgcct ttcagtgcct
     4561 cacagggcgc agcgcgcgcc agcagccgcc cggaggctgg cccgcctttg ggtccatcgt
     4621 ctcccgcctg caaggcaccg tggacaaagc catgccgcca tttgtcggcc tctcaccgaa
     4681 gatggggcac atgccctggg cgcgcaatgg cgagccgggt tacctgggcg tggcgcacgc
     4741 gcccttcgag ccgaaccgcg gcggcggcac cgatgacatg accctgcgcc gcatgacgct
     4801 tgaccgcctg catgaccgcg ccggtttgct caagagcttc gaccaactgc gccgcgacct
     4861 cgacgcatcc ggtctcatgg agggcatgga cgccttcaac gagcaggcgc tcggcgtgct
     4921 caccagcccg aagctgctgc aagcgctcga cctcacccgc gaggacagcc gcatcgccga
     4981 acgctacggc aagggcgaga acaaaaaccg cgacgacggc ggcccccgcc tcaccgacca
     5041 tttcctcgcc gcgcggcgtc tcgtcgaggc aggcacgcgc gtcgtcacgc tcggcttcag
     5101 ccgctgggac taccacagca acaacttcgg ccagctccgc gaggacctgc ctctgctcga
     5161 cagcgccctc agcgccctgc tcaccgacct gcgggagcgc ggcatggata aggacgtggc
     5221 cgtggtcgtg tggggagagt ttggccgcac cccggtcatc aacgcccagg gcggacgcga
     5281 ccactggccg cgcgtctcat gcgccatgct cgccggcggc gccttccgcc acggccaggt
     5341 catcggcgcc accgacaaag tggccggtga ggccaccgag cgccccgtgg cctttggcga
     5401 ggtctttgcc acgctctatc atcacctcgg cattgatgtg ggcaaagtca ccctcgacga
     5461 cttcagcggc cgcccgcaat acctcatcga aggcgcgcac cagccgctgg ccgagttggt
     5521 gtgaaaggct gagcaaatcc tcgcgcctgc gttaccatgc gcccttgact cccccggcta
     5581 ggccgctgtt tactcgcgcc catgcccgcc gccaaagcgc ccgccagttt ccgccgactg
     5641 ttcgatgaag tcggcaccgt ggatcatgtc ggcatcgagg agcgcgtggc caaatacacc
     5701 acgcgcagca tcaaaaagaa atcgaaggtc ttcggcctgc gtctcgccgt gacgatggtc
     5761 gatctgacca cgctcgaagg caaggacacg ccgggaaagg tcgcctcgct ctgccagaag
     5821 gcgctcactc ctcatgacga gggtgacatt cctccaactg cggcagtgtg cgtttatccc
     5881 gcgatggtga ggcacgcgaa gaagcatgtc ggcggcacga aggtgaaggt cgcctcggtg
     5941 gcgacggctt ttccatcggg acaagcgccg ctcaaaacac gcctcgccga agtccgcgcc
     6001 gccgtgaacg atggcgcgga tgaaattgac atggtcatca atcgcggagc tttcctcagt
     6061 ggcgagttcg gcctcatgca ggatgagatc gccgccgtgg tcgaagcctg tggtgagacg
     6121 acgctgaagg tcatcctgga agtcagcgaa ctggaaacct acgacaacat ccgcgccgcc
     6181 agcttcctcg ccatgcgtgc gatccgtccg ggcgacttca tcaagaccag caccggcaaa
     6241 acatcctcca acgccacgct cggcaacaat caggtcatgc tggaggccat ccgcgatcat
     6301 ttcctcgcca ccggcgttcc catcgccatg aagcccgccg gcggcatccg cacggcgaag
     6361 caggcgctgc atttcctcat cgccgtcaaa gagaccctcg gtgacgaatg gctcaacaac
     6421 cgccgttacc gcttcggcgc gtccgccttg ctcaacgatc tcgtgcgcca gctcgaaaaa
     6481 gaacgtaccg gagcctacca agccccgtgg aacttcagcg aggcgacgga aggctattga
     6541 tcccgttttt gtatgccgaa gacactctcc ccatccaaac ccaagaccaa tcccgcccgc
     6601 gaactcgtgt ttggcgatct gtgggagttt gatccggcac cggagacggc ggatccgaag
     6661 ctgaagaaga aatacgacat cttcatcaat ggtcgcttcg ttccgtcgcg ctccggcaag
     6721 tacttcgcga cgatcaatcc ggccacggag gtcaaaatcg ccgacattgc gctcgcggat
     6781 gcttcggatg tcgatgcggc ctgctcggca gcgcagcggg ccttcatcac ctggggccgt
     6841 ttgcccggca aagaacgcgg gaagtatctc taccgcatcg cacggctgct gcaggatcat
     6901 gcgcgggagt tcgccgtcgc cgagacgatg gatggcggga agccgatcaa ggaatcgcgt
     6961 gacttcgacg tgccgatggc cgcagcgcat ttcttttatc acgcgggttg ggcggacaaa
     7021 ctggagtttc tcgcgccggg tcggcaactc gcccctcacg gcgtcgtggg gcaggtgatt
     7081 ccatggaatt ttccgctgct catgctcgcg tggaagatcg cacctgcgct cgcggcgggg
     7141 aattgcgtcg tcttgaagcc ggcggagacc accagcatca ccgcgctgaa gtttggcgag
     7201 atttgccagg aagctggatt gccaaatggc gtcgtgaaca tcgtcagcgg tgcgggcgag
     7261 accggcgcgg cggtgatcaa tcatccgctc acctcgaaag tggccttcac cggcagcacg
     7321 gaggtcggga agatcatcat gcgcagcctc gcgggcacgg acaagaagct cacgctcgaa
     7381 ctcggcggca aggccgcgaa catcgtgttt gacgacgcgc cgctcgacca ggccgtcgag
     7441 ggcatcgtga acggcatctt cttcaatcaa ggccacgtct gctgcgcggg ctcgcgactg
     7501 ctcgtgcagg aaagcatcgc cgatgaattg ctcgccaaac tgaagcgccg catcgccacg
     7561 ctgcgagtcg gcgatccgct cgacaaaaac accgacctcg gtgccatcaa cagcgccgag
     7621 cagctcgcca aaatttccga cctcgtcaaa agcggtgtcg aggaaggcgc ggagaagcac
     7681 gacaacggct gcaaactgcc cgcgaagggc ttcttcttcc gtcccacgat cttcgcgaac
     7741 gtcagcatga gccaccgcat cgcccgcgag gagattttcg gccccgtgct cagcatcctc
     7801 accttccgaa cgctcgaaga agccgtcgaa aaagccaaca acaccgccta cggcctcagc
     7861 gccggcgtct ggaccgacaa aggcagccgc attctcaaaa tgagcacgct cctcaaagcc
     7921 ggcgtcgtct gggccaacac ctacaacaag tttgatccca cgagcccgtt cggaggattc
     7981 aaagagagcg ggtttggccg cgaaggcggg aggcaggggc tgctggatta tctcaccgtt
     8041 cactgaagta aaaccacgaa tgaacacgaa tacacacgaa tgtttttctg atgagctgct
     8101 gcatggcaaa ctcgtgtact cgattgtggg cgctgctttt gaggttttga aagggttgcg
     8161 gcatggcctg catgaaaagc cgtatgagaa cgcgatggtg gtggagctgc gactgcgcgg
     8221 gcatgcgctt gatcagcaga gacgctttga agtcgcgtat aaaggcgagc atgtcggcga
     8281 gttcgttccc gacctcatcg tggatggagc cgtggtcgtg gacgccaagg tgattgaccg
     8341 catcaccaat ctggaacgcg gccagatgct caattacctt cgcatcacag gtctgcgcgt
     8401 cggcctcatc atcaattttc acaagcccaa gctcgaatgg gagcgtgtca ttctctgatc
     8461 caatccattc gtggttcaaa aaatcttatg tcccgccttc acatcaccaa aacccccaaa
     8521 tgctacgtcg gcggtgcttt cattcgcagc gaaagcgggc gtgtgaccga gttgcatgac
     8581 gcttctggcg cgtttttcgc caacatcccg cagtgcacgc ggaaggacct gcgcaatgcc
     8641 gtggaagccg ccgcgaaggc ggggccgggg tgggcgaaga ggtcggcgtt caatcgcggg
     8701 cagatcttgt atcgcctggc ggagatgatg gaggcgcggg gcgtcgaact ggcggacagc
     8761 atcacgttga gcggtgtttc gaaggcggag gcctcgcgcg aggtcgcggc ggcgatcgag
     8821 cgcgtggtgc attacgcggg ctggacggac aaatacgagc aggtgctcgg cagcgtgaat
     8881 ccggtggcct cggcgcactt caatttcacc gtgacggagc cgatgggcat cgtcggcatc
     8941 ctcgcgccgg atgaagcgcc cgttttgggc ctgctgtcgc tgattttgcc cgcgatcacg
     9001 agcgggaaca gcgtcgtggc gctcgccagc acgacgcagc cgtatccgag catccttttg
     9061 ggcgaaatgc tcgccacgag cgacctgccc ggcggcgtgg tgaacctgct cactggcgtg
     9121 cgcagcgagt tgatccccac gtttgccacg cacgagcatc tgcgcgccct cagcggcgta
     9181 gcgaatgccg acgaacgcaa ggcgctgcgc ctcggcgcgg ccgaaagcgt gaagcgcgtg
     9241 cacttgcgca aggccgagga gcccatcgac tggttcgcgg acaagatgca gggcgcggag
     9301 gagattcgtg cgctggtcga gttcaagacg acgtggcatc cggtgggcgg ataaatgtct
     9361 tgaagcggaa atggttcacg ctgcccaaac ctcactttga gaactgggcg atcagcacca
     9421 ccacgagcag cgcgaggatg acggcgatgg cgatcttcac ccagcttagc ggatactcgc
     9481 cagtgatgcg tcccgtggca ccgttgacgg tgacttgata atttttcttc ccgtaggtgt
     9541 agctaagcag ccacacgggc agcagcacat gcttgaaggt ctggccggtg taggcgggag
     9601 agatgcggag gttgcggtga gtgtcgccag gcacgcgcgc ggcgcacatg gagcgcagtt
     9661 cgtcatccat tcgctcgcgc gagtgcttgg cggcctggat aaggtccagt tgatactgct
     9721 ccaccaccca gccggagagg tagccagcat cataggggac gaggtcggtg gtcgttggga
     9781 aaggctcgag gcctttgaga agctttgaat gcacgccttt ggacgcgggg acgagcacgt
     9841 cgtcaaagct gtgtttgact ctgccgctgg cgctttccca tcgggtgcgg cgctgctggc
     9901 ggccctggct gtcgcgtgtg taatagtggt aacccgcctc cgcctgccag gggcagtcgg
     9961 catgggcgtc aaaggtccag taaggcaggt agagtccgtg cagcgtgtcg gtcagcgcct
    10021 tgtccttgag atcacgtcgt gcccagaagt ggctgccata ccagcggcgg atgttctccc
    10081 gcacctggct ttgggagact ttgaagggca gcaggctgcc gggacggatg ggtgcgccgg
    10141 tgtcattcag cggcagcagg gcgggggagc cgcagaagtc gcagtttttg gcgacatggg
    10201 cggcgtcgaa aacggaaatg gcctgacagc tctggcagcg cacggatttg cgctccgcct
    10261 tccatccgcg ctcgtcatcg ggaatggcgc gcagggcggc ggccagatcg ctttcctcca
    10321 cgaggctgcc gtcggccttg agttcggcgg gggattcggt gccgcagtaa gggcagacaa
    10381 gcgcctgttt cgagggcgtc cacaccgcct ccgcgccgca ggctggacag gagaatttat
    10441 gaatcgcggt cgtggtttcc gtcattggct gccattctgc cgctgccggt tcggcgatca
    10501 aattcaaact cattcgattc gtcgtgatcc gcgcgaacta ccagcgtggc ggaccgttaa
    10561 acacaggtca tttcacgctc catccagccc ggccgctcac atgcgcatcc aacgaatccc
    10621 gtccctcctc cttccggttt tcatttcatt cccactgctc gcggctgccg ccggtttgcg
    10681 ggctgcggcg ccgcatgtga cgggaggcaa acccctgccg cccctgcctg agggggcctt
    10741 cactttggtg gtgatcccgg acacgcaatc ctacgcgggc aaaggctgca aagccacgcc
    10801 tgacagcacg gattcagtga ccaacagcaa cctggaggcg caggttcagt ggatcaccgc
    10861 caaccgcgag gcgcagaaca ttgtcttcgt cacccatgtc ggcgacatcg tggaccaaaa
    10921 caacgacgag caatggtcgg ttgcgaagcg gcatctcgac caactgcgtg gcgtggcgcc
    10981 gttcgcgctc acggtgggca atcatgacat gaccagcaat ggcgacgcgc ggctgttcca
    11041 gcggcatttt cccgccgcga gcttcgcgga tcaaccctgg tatctcgaat cctaccgcca
    11101 tgaccgcagg gatcagcacg tctcggcgga caacgtcaac agcgcgcagg tgttcagcgc
    11161 cggcggcctt gatttcctgc acatgagcct ggagtgcaac gcgccggatg atgtgctggc
    11221 ctgggcggac gcggtgctgc gcaggcaccc ggaaaggctc gccatcatca ccacccacat
    11281 ggacctcggg atcgagaaca aacccgcgac cacggagggc tatatccatg atccgaaggg
    11341 ccgcatgcgc tggacgaaaa tccacgggga gcgcggcaac agcggcgcgt ggatgtgggg
    11401 caagctttac cgcaaacatg ccaatctgcg cttcatcctc tgcggagacc aaagccgcgt
    11461 caccgccctc cgccgcgagg acagggctga tgacggccac gtcattcatt ccctgctgag
    11521 cgactacatg agtcagccgg tgctgcgtct catgcgtttt ttacctgcgg agcagcgcgt
    11581 ggatgtgctg acttatgagg tggggcgggg ttttctcgtg gagagcgcgc tccatgtgcc
    11641 ggaaaccggc cagcatcagt tttcgctgca tcttcctctg cggccgggcc gcgccccgtc
    11701 gtcatgatga tgcgaggcgc aaggcgcgga gagaagccaa attcggcggg atcacttgag
    11761 cacggggcgc agcagcatgt tgcggtagtc cacgttggta tggtcgccct ggaggaagat
    11821 gggcccgggc tttgactcgt cgctggtcag ggcgcctccg gtgcagccga gcacgggctg
    11881 gttgtctatg atggtggtgc cgttgaggat gacggtgagg tggcggtcca cgagggtgat
    11941 gtcgagggtc tgccattcac cgacgggttt ttcggcggcg actgagggcg tgatgcggct
    12001 gtaaagagcg cccatgtggt gcgagtccac gggtttgcca aagctctcca tgacctgcac
    12061 ctcatagatg ccgcgcaggt agatgccgct gttgctggcc tcctgggtgc ggacttcgag
    12121 agtcagattg aagtcttcga actctgcgcc ggtgcgcaga ttgccaaagt gtttgccggg
    12181 ctccttgacg acgcggttca tgagcacgcc gtcctccact ttccagccgt tgaggtcggc
    12241 ttcgttgacc agccgccagc cgctgagatc actgccgttg aaaagcgaca ccggcatgcc
    12301 gaacttgatt tttgccaggt ctggacgcgg cggcagcgcc gggatgcgtt tgccagtgaa
    12361 ctcgccctgg ccgaattcct tgccgtcatc ctttgccttc acggtggcac cggtaagcgc
    12421 gtccccgctg acttgcacgg tcagcgtctc ggtgactttt tcgttgatct ccccgccggc
    12481 cttgttcttg cgtttggcat tggagaggcg tgtgacgagc agtttgccat ccttgatctc
    12541 cgccccctga gcgggcacga cactgccgcc gccccagagc acgctggcgg tgagcgctcc
    12601 gtccttttcc tcgacgccga gccatccggc gcgtccgccg ggcagttcca aagcccagcg
    12661 tccgagatac ggattgtccg cggcaatggc agaggaggtg agaaacagcg cggcgaggag
    12721 atgtgtgatt ttcatttggg tcggcagttt ggctggcaat gcagatgtag ccggaagatc
    12781 gcggatgaaa agaggagatg tcactcacgc gccgcatgaa caatgttggc gacagcgaag
    12841 aagtacgcca tactgcgcgc ccatctcccg aacatgaccg aatctgaagc gactcccgac
    12901 aaccctgcgc agccttccgc cgcgcaggtg gacctctccc aactgcgcat gatgcccgcc
    12961 tgggtcgccg gcatgagcag ccttgcgaac ccggccaagt atgaggagcg cgaggaacgg
    13021 gaagaacgcc gcggccagcg gcccggcggc agggaccgca gagacggtcc gccaccccgc
    13081 agagacggtc cgccgccgcg ccgt
//
LOCUS       DAOMYJ010000147        13053 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Prosthecobacter sp. isolate AMR_MDS_5629
            NODE_1598_length_13053_cov_19.717187, whole genome shotgun
            sequence.
ACCESSION   DAOMYJ010000147 DAOMYJ010000000
VERSION     DAOMYJ010000147.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867850
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prosthecobacter sp. (wastewater metagenome)
  ORGANISM  Prosthecobacter sp.
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Verrucomicrobiaceae; Prosthecobacter.
REFERENCE   1  (bases 1 to 13053)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13053)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.30
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:34:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,742
            CDSs (total)                      :: 4,684
            Genes (coding)                    :: 4,676
            CDSs (with protein)               :: 4,676
            Genes (RNA)                       :: 58
            tRNAs                             :: 55
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13053
                     /organism="Prosthecobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1598_length_13053_cov_19.717187"
                     /isolate="AMR_MDS_5629"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1965333"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            266..5329
                     /locus_tag="PLP58_14640"
     CDS             266..5329
                     /locus_tag="PLP58_14640"
                     /inference="COORDINATES: protein motif:HMM:TIGR02604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ThuA domain-containing protein"
                     /protein_id="HRJ09812.1"
                     /translation="MKRHLCALLLSALCALPASAAEPLRVFIRAGAKSHGPGAHDFPQ
                     FLKDWVPMLNERGAKAEGGLEFPTKEQLDRTDVLVLHAQEAGNIKIGEERKNLMEFLK
                     RGGGLVVIHAAAVSRDHDWFKTIIGGSWKFGQTKWLEAPLSLYFTDRENPITRHISNF
                     DLDDEIYYDMEILPEAKVLAAAYTPKAIDTGGKGNKEAQERAAEAVAKKKGVNIYDIQ
                     PQVWTYEKAWGSGVSPDPSTGGTPVPRTTTYRAFTCIPGHWYKNFSHNGLRAMILRGI
                     AWAGKRDNVDELCKPEELGDALRYVEGGAPRPQDLPKHLEVHPEFNLSLVASEPLINN
                     PMNIDWDEKGRLWVVETPEYPNGLRQANVESWMDSGSLKPGQYARDPEDRISILEDTN
                     GDGVMDKKTVFADKIELATSSVFYKNGVIVCAAPDIWFFEDTNGDDKADKRTKLYTNL
                     GIRDTHAVINNLRWGLDGWIYATHGYSSSDNVTSGDGSKNFGLIGSGVVRFKPDGSAF
                     EQYCSKGGNTWGLDITWDGQVFYTQPTSGVLFHHVVLPEYVLAKGKLPGVTSSNGLLA
                     REPTYPAMHWEQQAYVQIDQVGSYTAAAGCAIYEGGAWPEKWNYGYFTTEPTLNIVSH
                     FMVEPDGVTYQAKREPGREQTEFIRSKNLWFRPIEVRVGPDGALYVVDFCNQAVIHND
                     TRGPTHGPANAAVRPDRDHYYGRIWKVQHKEAKKVEVVKMDNDNLQPLQNIIAEAKNK
                     HQSLTAKRLLQQKGTDYRVVSSSMTANPLSYFELASHAADPAQRMQMMTMYAYATDDW
                     TRSAIIAGASGNPVDLIPPSLAFGEAEKLVSMVEALIPAAVKQDGVAGVSKLMTACAN
                     ADRKADPLKTAILRGISQQFNEAPELTPALTDSLKKLLAQPALAAATLPLVTKWDKGG
                     VLAGEVKTQLAKLEATLSDNTAPAEARINAARALIAVGGANTEVGVKPLGDTETPEEL
                     QAAIIQALDEKGDAGELVASLNRLKPRLQQMAFEAILKRPEATRALLDAVKAGSIDRA
                     AFAPGDVARLRSHPNKPLARRANELFQVNNAAKAAIIAKLTPEVEKPGNAENGKLLYS
                     AACAVCHKLGDLGMAVGPPLDGMGAHGPAELLIHIVDPNREVDPSFWAFNISTKKGEL
                     LQGVITSENNATVTLATQAGVREIAKSDIEKRENTGRSLMPEGLDGLGAEALRDILTF
                     ICGEASKQFRIISLADAATASSSDGVFAGPAPNQGQVRLKKFGDFKVEGVPFFIPSPE
                     KATAGNIIVLKGGPAPDNHSQTFPARVELALNVETKKLQLLSGISGWGWPAVRDEAPA
                     LKATIVYASGEKEEFTFINGVHFSDYIRPTEVPGSRYVEGITDGQQMRLLTLELTKKG
                     PAKTLILESPAGNKTAPVIAAITADIEGRAGFQPATSGPGILPGKAKGPGKKKAGKMP
                     ATPESGGTPELQPKSGGQGDGPLVPASTPQWEPGKTKVLVIGGGSSHNFKDFFGTTDV
                     ATLKAAGFSVHYTEDRDQAATELANADVAVISVNRKFFDTAAYRKALFDFAAAGKGII
                     MLHPGTWYGYANWPELNAQIVGGGARGHDKIHPFDVKKLKDHPIMEGVPASFTVEDEL
                     YYINAEPEKIPAGTAPIEVLAETSPSDKFQKSHPSVWITEHPKARIVGIALGHDERTH
                     DLEPYRKLLANAVKWGAGK"
     gene            complement(5366..6367)
                     /locus_tag="PLP58_14645"
     CDS             complement(5366..6367)
                     /locus_tag="PLP58_14645"
                     /inference="COORDINATES: protein motif:HMM:NF017181.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ09813.1"
                     /translation="MAVPNLTNILKRILSKPTAPFHEYHVRAEIEALLKDCPHVKLKH
                     DKFGNLLATYKNGKSKSKPTWVLGAHMDHPGFVRVPGGGKNDFDFLGGVSQTQVESGI
                     ARGLRGKTQGAIATWKFPVKIGHTKIEATACDDLVGCATIVATFWELASLGISTTFHA
                     AFTRAEEVGFLGAWHLAQKWPFGKDDVFLSIETSRPVNGAVMGGGPVVRVGDRLSIFD
                     SEGCAVLMTTAKEQGIRVQRCLLDAGACEASAMQAAGHRSCGISIPLANYHNLDADGQ
                     IAPEHVMMSDVRSLIDLLKALVATRHDGIGERTIRERVELRSEEYAAHLKAANKLCK"
     gene            6411..7367
                     /locus_tag="PLP58_14650"
     CDS             6411..7367
                     /locus_tag="PLP58_14650"
                     /inference="COORDINATES: protein motif:HMM:NF015251.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HRJ09814.1"
                     /translation="MKKLRYFCETCLVASAAWLLPRMPRGLILALARGIGSLAYLLDA
                     RGRRTALENLRAAFGDAFSEKRRAAVARGSYQNFARTFLDLFWSARLTRENWPEHVTI
                     HHGAEDAEARARRVGALWVTPHFGNFELASLFWGFRDFKFTVVAQDFKNPALTGIFKR
                     LREHSGHTVIPQQGAMLRLMKTLKRQGHAALLTDLNVEPGRAATVIECFGLKTCVTTL
                     HVQLARRLGLAIIPGVCLPMPDGRYEVHIFEAIEPADGQTDRQLAQRVWDHFEKAIRE
                     HPERWLWMYKHWRYQPARGRDPAYPDYAKANGDFWKMVEGTS"
     gene            7419..7865
                     /locus_tag="PLP58_14655"
     CDS             7419..7865
                     /locus_tag="PLP58_14655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ09815.1"
                     /translation="MTRFFEILGRFDHEGDCQKLLYAPPAQRLTFRETVLYKIEFDGD
                     EAALESFVRQVLLDPVSQDLRDGSTPAFDKASFTLEYGMKGGALDLEKETILGYYRAL
                     ENPGFALQKLTLRRRVYVFGDGAVPAPFIRDICNPAIHTWEVRQAA"
     gene            7932..9011
                     /locus_tag="PLP58_14660"
     CDS             7932..9011
                     /locus_tag="PLP58_14660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009962408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--glyoxylate aminotransferase family
                     protein"
                     /protein_id="HRJ09816.1"
                     /translation="MSKHIKLYIPGPVEVSPATYEAMCQPMIGHRGKGFQDLYAEIQP
                     MLRQLFGTRQQVFLSTSSAWGVMEGSIRNLVKKKVLNCCNGAFSDKWLDVSRRCGKEA
                     EAYQVEWGRPILADEVDKRLATGEFDAVTFIHNETSTGVLSPIAEIAALKRKYPDVMF
                     ITDSVSGFTTVPVSFDELGLDVLLTGSQKAFALPPGLALFTASEAAMERAAGIHDRGY
                     YFDFLEFRSNGEKNMTPSTPCISLIYGLRHQLQQMLAEGLDNRYARHARLNGMVHDWV
                     RRNGFEFFAPEGYRSKSLTCVANNRNIDVAAFIGLLKSNHSLIIDGGYGKLKGRTFRI
                     SNMGDETDASISAVIAALDDSLAKL"
     gene            9171..9467
                     /locus_tag="PLP58_14665"
     CDS             9171..9467
                     /locus_tag="PLP58_14665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ09817.1"
                     /translation="MPFRPPGQCPVCGEWVPRGAAACDDCGACAKSGWKDDAETYDGL
                     DLPDEDFDYDDFIKREFDNRARQLGGPTREQLWRWVGLAVFIVMVLGYLISVFR"
     gene            9477..10292
                     /gene="lpxI"
                     /locus_tag="PLP58_14670"
     CDS             9477..10292
                     /gene="lpxI"
                     /locus_tag="PLP58_14670"
                     /EC_number="3.6.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009963428.1"
                     /note="LpxI, functionally equivalent to LpxH, replaces it
                     in LPS biosynthesis in a minority of bacteria; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-2,3-diacylglucosamine diphosphatase LpxI"
                     /protein_id="HRJ09818.1"
                     /translation="MSAPDFSHLALIAGNGIYPGTMVAAARRAGVRRLVAAAFVDETR
                     PELASQVEAIEWFRVGQLGKMIAFFKREGVSQAVMVGQIAPKNLFDLRPDLRTLLMLA
                     RVPKRNAESLFGAIAGELAKDGITLLPATTFLEDLMPAAGHVAGPQIKKRRWDDARYG
                     FDIAKESSRLDIGQTVVVKNGTVLAVEAFEGTNEAVKRGGALGRGGATMVKVSKPDQD
                     MRFDVPVIGPDTIRTAAAAGVDLIAIEAERTLLLGREELVRECQTLKVALIAL"
     gene            10383..11153
                     /locus_tag="PLP58_14675"
     CDS             10383..11153
                     /locus_tag="PLP58_14675"
                     /inference="COORDINATES: protein motif:HMM:NF023218.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HRJ09819.1"
                     /translation="MKMPRLISRRLFTTGLASCLGGTRGGLAAEAAAGKKGWGGADAE
                     LHKLFKVHWHYNWMPRGTSTQEVEFVPLIKGTPALNQAQVIVETPGVKHLLGFNEPER
                     ESQGNVSVSEALDLWPKLEAIAEKAGLRLGSPATSSDKPGMDWFEAFMEQAKRRKLRM
                     DFVALHWYRGRDASAFESFVTAISRSHRLPVWVTEFNGWSGGEEENHEFLKGTLRFME
                     RSRIVERYAYFNPPKGAPHSLLAADGSLTRMGKLYSET"
     gene            complement(11175..12101)
                     /locus_tag="PLP58_14680"
     CDS             complement(11175..12101)
                     /locus_tag="PLP58_14680"
                     /inference="COORDINATES: protein motif:HMM:NF016777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HRJ09820.1"
                     /translation="MNRRRLLQATAAAALLPSTQRAAPAATRVIDTHTHFYDPARPQG
                     VPWPPKNSPLHRTVLPADWLAVAAPHGARETVVVEASQWVEDNQWVLDLAEKEKSIVG
                     LVGNLDPNDAQFAANLRRFAANPLFRGIRWRGDLVRLDENRDKVLAGARLLAEHGLEL
                     DLNGPASALEHAARLAAEVPDLRVVINHLGASGDPRALKPEWREAVRAVAAGPNVFMK
                     VSALVEQVRDADGKAPDDAAFYLPILDHLWDCFGEDRLIYGSNWPVSDRGAPYETVFK
                     IVSDYFGSKGREAAEKYFWRNSLKAYRWIERR"
     gene            12208..>13053
                     /locus_tag="PLP58_14685"
     CDS             12208..>13053
                     /locus_tag="PLP58_14685"
                     /inference="COORDINATES: protein motif:HMM:NF016408.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymer-forming cytoskeletal protein"
                     /protein_id="HRJ09821.1"
                     /translation="MFDPFTTLQETGFSQGADSARPGGWTPELIAALDWMRVAELARA
                     IAGHNGCELAGSRVLEDASVVFAMLERPTTAHPQRALVKTAGWNEWGATPESVRRFST
                     EVATAKNTRGILIAPGGFSPAALLAAQAARIEAVDAAALHAVLMRLPAERSDFFFTIA
                     TAGDCRRPSCPVCLRKLERSGKDDAAAQDEHRTIRAEGLVADPVECGTLSVPAGAEVT
                     FLYEVRARRVHIAGHAQGDFACEGTVIIEPGGILTGTVTARAVEVREGGELRGQFRIL
                     EGALRP"
BASE COUNT         2508 a         4332 c         4026 g         2187 t
ORIGIN      
        1 catccgttga ctgatcatcg accccagcgc cgccgcgcca atgccggtgg cgccgcgccc
       61 aaacaaggcg cggcgggtga gctgggcacg atgatcagtc aacggatgca tgatgaatgg
      121 cggaaaaaac gcaattccac gaggcgatat tccaccgcgc atttgcagta tgcgcgcggc
      181 cgcggcgcat gtgcattccc gatagcgcgc cgtgcgaaaa cgggcttccc agcgggcgtt
      241 cgtttgtcat acgctatttc cagccatgaa acgccacctt tgcgccctcc tcctttccgc
      301 tctctgcgcg ctgcccgcct ctgccgcgga gccgctgcgt gtcttcatcc gcgccggtgc
      361 caagtcgcac ggcccgggcg cgcatgactt cccgcaattt ctcaaagact gggtgcccat
      421 gctcaacgag cgcggcgcca aggccgaagg cggcctggaa ttccccacca aagagcagct
      481 cgaccgcacc gacgtgctcg tcctgcacgc gcaggaggct gggaacatca agatcggcga
      541 ggagcggaag aatctgatgg agttcctcaa gcgcggcggc ggtctggtcg tcatccacgc
      601 ggcggcagtc tcgcgcgatc atgactggtt caagaccatc atcggcggct cgtggaagtt
      661 cggccagacg aagtggctgg aggcgccgct gagcctgtat ttcaccgacc gcgagaatcc
      721 catcacccgg cacatctcga acttcgatct cgacgacgaa atctactacg acatggagat
      781 cctccccgag gcgaaggtgc tcgccgccgc ttacacgccc aaggcgattg acaccggcgg
      841 caagggcaac aaagaggccc aggagcgcgc cgctgaagcc gtggcaaaga agaagggcgt
      901 caacatctac gacatccagc cgcaggtgtg gacctatgag aaagcatggg gatcaggcgt
      961 ctcgcctgat ccatccacag gcgggacgcc tgtgccccga acaaccacct accgtgcctt
     1021 cacctgcatc cccggccact ggtacaaaaa cttttctcac aacggcctgc gcgcgatgat
     1081 cctgcgcggc atcgcctggg ctggcaagcg cgacaacgtg gacgagctgt gcaagcccga
     1141 ggaactcggc gatgccctgc gctacgtcga aggcggcgcg ccgcgcccgc aggacctgcc
     1201 gaagcacctg gaggtgcatc ccgagtttaa cctctccctc gtcgcctccg agccgctcat
     1261 caacaacccg atgaacattg actgggatga aaaaggccgg ctctgggtgg tcgagacgcc
     1321 cgagtatccg aacggtctgc gccaggccaa tgtcgagtcc tggatggact ccggctcgct
     1381 caagcccggc caatatgcgc gcgatcccga ggaccgcatc agcattctcg aagacaccaa
     1441 tggcgacggc gtcatggaca aaaagaccgt ctttgccgac aagatcgagc tggccaccag
     1501 cagcgtgttt tacaaaaacg gcgtcatcgt ctgcgccgcg ccggacatct ggttcttcga
     1561 ggacaccaac ggcgacgaca aggccgacaa gcgcacgaag ctctacacca acctcggcat
     1621 ccgcgacacg cacgccgtca tcaacaacct gcgctggggc ctcgacggct ggatctatgc
     1681 cacgcatggg tattcgagca gcgacaacgt cacctccggc gacggcagca aaaacttcgg
     1741 cctcataggc agcggcgtcg tccgcttcaa acccgatggc agcgcgtttg agcaatactg
     1801 ctccaaaggc ggcaacacct ggggtctcga catcacctgg gacggccagg ttttctacac
     1861 ccagcccacc agtggcgttt tgttccatca cgtcgtcctg cccgagtatg tgctcgccaa
     1921 aggcaagctg cccggcgtga ccagcagcaa cggcctgctt gcccgcgagc ccacttaccc
     1981 cgccatgcac tgggagcagc aggcctatgt gcagatcgac caggtcggca gctacaccgc
     2041 cgccgccggt tgcgccatct atgagggcgg agcctggccg gagaaatgga actacggcta
     2101 cttcaccacc gagcccacgc tgaacatcgt cagccacttc atggtcgagc ccgatggcgt
     2161 cacctaccaa gcgaagcgcg agcctggccg cgagcagacc gagttcatcc gcagcaagaa
     2221 cctctggttc cgccccatcg aggtgcgcgt cggtcccgac ggcgcgcttt atgtcgtgga
     2281 cttctgcaat caggccgtca tccacaacga cacccgcggc cccacccacg gcccggcgaa
     2341 tgccgccgtc cgccccgacc gcgaccacta ctacggccgc atctggaaag tgcagcacaa
     2401 ggaggcgaag aaggtggagg tggtgaagat ggacaacgat aatttgcaac cgctccagaa
     2461 catcatcgct gaagccaaga acaagcacca gagtctcact gccaaaaggc tgttgcagca
     2521 aaagggcacc gactatcggg ttgtctcgtc gtcgatgacg gcgaacccct tgtcatattt
     2581 cgaactcgct tcacatgcgg cggacccggc acagcgaatg caaatgatga caatgtatgc
     2641 ctatgcaacg gacgactgga ctcgatcagc catcattgct ggcgcatcag gaaatccagt
     2701 ggatttaatt cccccgtctc tcgcatttgg agaagccgaa aagttggtgt ccatggttga
     2761 ggcgctcatc cccgcagccg tgaaacaaga tggagttgct ggtgtgtcaa agctcatgac
     2821 tgcgtgtgcc aatgccgaca ggaaggctga tccgttaaag accgccatcc tacgcggcat
     2881 ctctcagcag ttcaatgaag ctccggagct cactcctgct ctcaccgact cattgaaaaa
     2941 actcctcgcc cagcccgcgc tcgccgcagc cacgttgccg ctcgtaacga agtgggacaa
     3001 aggcggtgtg ctcgccggtg aggtcaaaac gcagctcgcc aagcttgagg ccacgctttc
     3061 cgacaacacc gcgcccgccg aagcccgcat caacgccgcg cgcgccctca tcgcagtggg
     3121 cggtgcgaat acagaagttg gagtgaagcc tttaggcgac acagagacgc ccgaagaact
     3181 acaggcggcc atcattcaag cattggatga aaagggagac gctggagagc tcgtggcgtc
     3241 attgaatcgg ctgaagccca gactccaaca aatggccttc gaagccatcc tcaaacgccc
     3301 cgaagccaca cgcgccttgc tggacgccgt gaaggccggc agcattgacc gcgcggcttt
     3361 cgcccctggt gatgtcgcac gcctgcgctc gcatccgaac aagccgctgg cccgccgtgc
     3421 caacgagctg ttccaggtca acaacgccgc caaggcagcc atcatcgcca agctcacgcc
     3481 cgaggtggaa aagccgggca atgccgaaaa tggcaagctg ctttactccg ccgcctgcgc
     3541 cgtctgtcac aagctgggcg atctcggcat ggccgtcggc ccaccgctcg atggcatggg
     3601 tgcgcacggc cccgccgagc tgctcatcca catcgtggac ccgaaccgcg aggtggaccc
     3661 gagtttctgg gccttcaaca tcagcaccaa aaaaggcgag ctgctgcaag gcgtcatcac
     3721 cagcgagaac aacgccaccg tcaccctcgc cacccaggcc ggtgtgcgcg agatcgccaa
     3781 gagcgacatc gaaaaacgcg agaacaccgg acgcagcctc atgcccgaag gtctcgacgg
     3841 cctcggcgcc gaggctttgc gcgacatttt gaccttcatc tgcggcgagg cctcgaagca
     3901 gttccgcatc atctcactgg ccgatgccgc caccgccagc agcagcgacg gcgtctttgc
     3961 cggccccgcg ccgaaccagg gccaggtgcg cctgaagaag ttcggcgact tcaaagtgga
     4021 gggcgtgccc ttcttcatcc cctcgcctga gaaagccacg gctggaaaca tcatcgtgct
     4081 caaaggcggt cccgcgcccg acaaccactc gcagaccttc cccgccaggg tcgaactggc
     4141 cttgaacgtc gagaccaaga agctgcaact gctcagcggc atctccggct ggggctggcc
     4201 cgccgtgcgc gatgaagcgc ccgcgctcaa agccaccatc gtctatgcca gcggcgaaaa
     4261 agaggagttc accttcatca acggcgtaca tttctccgac tacatccggc ccaccgaagt
     4321 gcccggctcc cgctatgtgg agggcatcac cgacggccag cagatgcgcc ttctcaccct
     4381 ggaactgacg aagaaaggcc ccgcgaaaac gctcatcctc gaaagccccg ccggcaacaa
     4441 aaccgccccc gtcatcgccg ccatcaccgc cgacatcgaa ggtagggcgg gctttcagcc
     4501 tgccacgagt ggaccgggca ttctgcccgg caaagccaaa ggccccggca aaaagaaagc
     4561 gggcaaaatg cccgccacac ccgagtcagg cgggacgcct gagctacaac ccaaatcagg
     4621 aggccagggc gatggcccgc tcgtccccgc cagcacgccg caatgggagc ccggcaaaac
     4681 caaggtgctc gtcatcggcg gcggctcctc ccacaacttc aaagacttct tcggcaccac
     4741 cgacgtcgcc acgctcaaag ccgccggctt cagcgtccac tacaccgagg accgcgacca
     4801 ggccgccacc gaactggcca atgccgacgt ggctgtcatc agcgtgaacc gcaaattttt
     4861 cgacaccgcc gcctatcgca aagcgctctt cgatttcgcc gccgccggca agggcatcat
     4921 catgctccac cccggcacct ggtatgggta cgccaactgg ccggagctga acgcgcagat
     4981 cgtcggcggc ggcgcgcgcg ggcatgacaa gatccatccc ttcgatgtca aaaagctcaa
     5041 ggaccatccc atcatggaag gcgtgcccgc cagtttcacc gtcgaggacg agctctacta
     5101 catcaatgcc gagccggaaa aaatccccgc cggcaccgcc cccatcgaag tgctggccga
     5161 gaccagcccg agcgacaaat tccaaaaatc gcacccaagt gtctggatca ccgagcatcc
     5221 gaaagcgcgc atcgtcggca tcgccctcgg tcacgacgag cgcacgcatg acctggagcc
     5281 atacagaaag ctgctcgcca acgcggtgaa gtggggggcg ggcaagtgag gttgggaaag
     5341 ccggcccggc cgttccactt gctgctcact tgcaaagctt gttcgccgct ttgagatggg
     5401 cggcatactc ctcgctgcgc agctcgacgc gttcgcggat ggtgcgctcg ccgatgccgt
     5461 cgtggcgggt ggcgacgagg gctttgagga ggtcgatgag ggagcgcacg tcgctcatca
     5521 tgacgtgctc gggggcgatc tggccgtcgg cgtcgagatt gtggtagttg gccaggggga
     5581 tggagatgcc gcaactgcga tgtccggctg cctgcatggc gctggcttcg caggcgccag
     5641 cgtcgagcag gcagcgctgg acgcggatgc cctgctcttt ggcggtggtc atgaggacgg
     5701 cgcagccctc gctgtcgaag atggaaaggc ggtctccaac acggacgacg gggccgccgc
     5761 ccatgacggc gccgttgacg gggcggctgg tctcgatgga gagaaagacg tcgtctttgc
     5821 cgaagggcca cttctgcgcc aaatgccacg cccctagaaa gcccacctcc tccgcgcggg
     5881 tgaaggcagc gtgaaaggtg gtgctgatgc cgaggctggc cagttcccag aaggtggcga
     5941 cgatggtcgc gcagccgacg aggtcgtcac acgcggtggc ctcaattttg gtgtggccga
     6001 ttttgaccgg gaatttccag gtggcgatgg caccctgcgt cttgccgcga agtccgcgcg
     6061 cgatgccgga ctcgacctgc gtctgggaga cgcccccgag gaagtcgaag tcgtttttgc
     6121 cgccgccggg aacgcggaca aagccggggt ggtccatgtg ggcgccgagc acccaggtgg
     6181 gcttgctttt gcttttgccg ttcttgtagg tggccaggag gttgccgaat ttgtcgtgct
     6241 tgagcttcac atgcgggcaa tccttgagca gcgcctcgat ttccgcgcgg acatgatatt
     6301 catgaaacgg ggcggtgggc ttgctcagaa ttcgcttgag aatgttcgtc aggttgggca
     6361 ctgccatgcg ggcacgctag cggccttttt ccatccgaca aacgacaaag gtgaagaaac
     6421 tccgatattt ctgcgaaacc tgcctggtgg cttccgccgc ctggcttctg ccgcgcatgc
     6481 cgcgcggctt gattctggcg cttgcgcgcg gcatcggctc gctggcttat ctgctggatg
     6541 cgcgcggacg ccgcacggct ttggagaatc tgcgcgcggc atttggtgat gccttttctg
     6601 aaaagcggcg ggcggctgtc gcgcgcggat cctaccagaa ttttgcgcgc acttttcttg
     6661 atcttttctg gtccgcgcgg ctcacgcggg aaaactggcc cgagcatgtg acgatccacc
     6721 acggcgcgga ggatgccgag gcacgggcgc ggcgggtggg cgcgttgtgg gtgacgccgc
     6781 acttcgggaa tttcgaactg gccagcctgt tttggggatt ccgggatttt aagttcaccg
     6841 tggtggcgca ggacttcaag aacccggcgt taaccggcat cttcaaacgc ctgcgcgagc
     6901 actccgggca caccgtcatc ccccagcaag gggccatgct gcggctgatg aaaacgctga
     6961 aacgccaggg ccacgcggcc ttgctcacgg acttgaacgt ggagcccggg cgggccgcca
     7021 cggtcatcga gtgttttggc ctgaaaacct gtgtgacgac gctgcacgtc caactcgccc
     7081 gccggctcgg gctggcaatc atcccgggcg tctgcctgcc catgccggac gggcgctacg
     7141 aggtgcatat cttcgaagcc atcgaacccg cggacgggca gacggaccgg cagctggccc
     7201 agcgtgtttg ggatcacttt gaaaaggcca tccgcgagca tcctgagcgc tggctctgga
     7261 tgtacaagca ctggcgctac cagcccgcgc gcggcaggga ccccgcttat ccggattacg
     7321 caaaggccaa tggcgacttt tggaagatgg tggaagggac ctcgtgaaca acatccggtt
     7381 tgcgccgccg cgctttccgg ctagaaactg ccagcccgat gacccgcttt tttgaaattc
     7441 ttggccgatt cgaccacgag ggcgactgcc aaaaactgct ctacgcaccc ccagcgcaga
     7501 ggctcacttt ccgcgagacc gtgctctaca aaatcgagtt cgatggagat gaagccgcgc
     7561 ttgagtcttt cgtccgccaa gtgctgctcg accctgtcag ccaggatctg cgggacggct
     7621 ccacgccggc ttttgacaag gcatccttca ccctggaata cggcatgaag ggcggcgcgc
     7681 tcgatctcga gaaggagacc atcctcggct actaccgcgc gctggagaat cccggctttg
     7741 ctctgcaaaa gctcacactc cgccgccggg tttacgtttt cggagacggt gccgtgcccg
     7801 ctccattcat ccgcgacatc tgcaaccccg ccatccacac ctgggaagtg cggcaggccg
     7861 cgtgagtccc gccaggagca tccgccgccc ctttcatcgc gtgtcttttg accgcccaaa
     7921 tttcatcccc catgagcaaa cacatcaaac tctacatccc cggccccgtc gaagtcagcc
     7981 ccgccactta tgaggccatg tgccagccga tgatcggcca ccgcggcaag ggcttccagg
     8041 atctttacgc cgagatccag cccatgctgc gccagctctt cggcacgcgg cagcaggtct
     8101 tcctcagcac ctcctcggcc tggggtgtca tggagggcag catccgcaac ctcgtgaaaa
     8161 agaaggtgct caactgctgc aacggcgcct tttccgacaa gtggctggat gtctcccggc
     8221 gctgcggcaa ggaggcggag gcatatcaag tggagtgggg ccggcccatc ctcgccgatg
     8281 aggtggacaa aaggctcgcc acgggtgagt tcgacgccgt cacattcatc cacaacgaga
     8341 cctccaccgg cgtgctcagc cccatcgccg agatcgccgc gctgaagcgg aaatacccgg
     8401 atgtgatgtt catcaccgac agcgtctcgg gcttcaccac ggtgccggtg agctttgacg
     8461 agctcggcct ggatgtgctg ctcaccggct cgcagaaggc cttcgctctg ccgcccggcc
     8521 tggccctttt caccgccagc gaggccgcca tggagcgcgc cgccggcatc catgaccgcg
     8581 gatattattt cgacttcctg gagttccgct ccaacggcga aaagaacatg acgcccagca
     8641 cgccctgcat cagcctcatt tacggcctgc gccaccagct tcagcaaatg ctcgccgaag
     8701 ggctcgacaa ccgctacgcc cgccacgcgc gcctcaacgg catggtgcat gactgggtgc
     8761 gccggaacgg tttcgaattc ttcgcgcccg agggctaccg ctccaagtcc ctcacctgcg
     8821 tggcgaacaa ccgcaacatt gacgtggcgg ccttcatcgg cctgctcaag agcaaccaca
     8881 gcctcatcat tgacggcggc tacggcaagc tcaagggcag gaccttccgc atctccaaca
     8941 tgggcgacga gaccgacgcg agcatcagcg ccgtcatcgc cgcgctggac gactctttgg
     9001 cgaagctgtg aggggttttg aaccactaat aaacactcat gagcactaat atcagaagcg
     9061 cttcgcgcgg aaagagtcag tggtcccgcc aaaacccgcc ggcgcgaagc gccaaacatt
     9121 agtgctcatt agcgtccatt agtggttcca aaacaccccg cccgaaagcc atgcccttcc
     9181 gacctccagg ccagtgcccc gtgtgtggcg agtgggtgcc gcgcggcgcg gcggcttgcg
     9241 acgactgcgg agcctgcgcc aagtccggtt ggaaggacga tgccgaaacc tatgacggcc
     9301 tggatttgcc cgacgaagac ttcgattacg acgacttcat caagcgcgag tttgacaacc
     9361 gcgcgcggca gcttggcggc cccacgcggg agcagctctg gcgctgggtg gggctcgccg
     9421 ttttcatcgt catggtgctg ggttatctga tctccgtctt ccgctgactt caccgcatga
     9481 gcgcgcccga cttctcccac ctcgccctca tcgccggcaa cggcatctac cccggcacca
     9541 tggtcgccgc cgcgcgccgc gccggtgtgc gcaggctggt ggccgccgcg tttgtggacg
     9601 agacccggcc cgagctcgcc agccaggtgg aggccatcga gtggttccgc gtggggcagc
     9661 ttgggaaaat gatcgccttt ttcaaaagag agggagtcag ccaggcggtg atggtcggcc
     9721 agatcgcccc gaagaacctc ttcgacctcc ggcccgatct gcgcaccctg ctcatgctgg
     9781 cgcgtgtgcc gaagcgcaac gccgagagcc tctttggagc catcgccgga gagctggcca
     9841 aggacggcat caccctgctg cccgccacca cgtttctgga ggacctcatg cccgccgccg
     9901 gtcatgtggc cgggccccag atcaaaaagc gccggtggga cgacgcccgc tacggtttcg
     9961 acatcgccaa ggaaagcagc cggctcgaca tcggccagac cgtggtggtg aaaaacggca
    10021 ccgtgctcgc cgtcgaggcc tttgaaggca ccaacgaggc cgtcaagcgc ggcggcgccc
    10081 tcggccgcgg cggcgcgacg atggtcaaag tctccaagcc ggaccaggac atgcgcttcg
    10141 atgtccccgt catcggcccc gacaccatcc gcaccgccgc cgccgccggc gtggacctca
    10201 tcgccattga ggcggagcgc acgctgctgc tggggagaga ggagttggtc agggagtgcc
    10261 agactttgaa ggtggcgctc atcgccctct gatcccaggc gccgccaccc acaaaggccg
    10321 ccccatgcag agtcgcaaaa gttcttttgc caccgcatgc cattcatcct agcctgagca
    10381 tcatgaaaat gccccgcctc atcagccgcc gacttttcac cactggtctc gcctcctgcc
    10441 tgggcgggac acgcggcggc ctggccgccg aggcggcggc ggggaaaaaa ggctggggag
    10501 gcgcggacgc ggagctgcac aagctcttca aagtccactg gcactacaac tggatgccca
    10561 gggggacctc gacccaggag gtggaatttg tgcccctgat caaagggacc cccgcgctca
    10621 accaggccca ggtcattgtc gaaacacccg gcgtcaagca cctgctcgga ttcaacgagc
    10681 ctgagcggga gagccagggc aatgtgtcgg tgagcgaagc gctggacctc tggccgaagt
    10741 tggaggccat cgctgaaaaa gccggtctcc ggctcggctc gccggccacg tcctcggaca
    10801 agccgggcat ggactggttc gaggctttca tggagcaggc gaagcggcgc aagctgcgca
    10861 tggacttcgt ggcgctgcac tggtaccgcg gccgcgatgc ctccgccttt gaatcttttg
    10921 tgaccgccat ctcacgctcc catcggttgc ccgtctgggt gacggagttc aatggctgga
    10981 gcgggggcga ggaggagaac cacgagtttc tcaaaggaac gctgcgcttc atggagcgca
    11041 gccgcatcgt cgagcgctac gcgtatttca atccgcccaa aggcgctccg cacagcctgc
    11101 tggccgccga cggcagcctc acccgcatgg gcaaactcta cagcgaaacc tgattatgtc
    11161 tgtgcctttc acattcaccg tcgctcgatc cagcggtagg ctttgagtga gtttctccaa
    11221 aaatacttct ccgccgcctc gcggcctttg gagccaaagt aatcactgac aatcttgaag
    11281 acggtctcat aaggcgcgcc gcggtcggag acgggccagt tgctgccgta gatgaggcgg
    11341 tcctcgccga agcagtccca caggtggtcg agaatgggca ggtaaaaggc cgcgtcgtcc
    11401 ggcgctttgc catccgcgtc cctcacctgc tcgacaaggg cggagacctt catgaagaca
    11461 ttcggcccgg ccgccacggc gcggacggct tccctccact ccggcttcaa agcgcgcgga
    11521 tcaccggagg cgccgaggtg gttgatgacg acgcgcaggt ccggcacttc ggcggcgagg
    11581 cgcgctgcgt gctccagcgc ggaagccggc ccgttgaggt cgagctccag cccgtgctcc
    11641 gccagcaggc gcgccccggc cagcaccttg tcgcggtttt catccaggcg caccagatcg
    11701 ccgcgccagc ggatgccgcg aaacaggggg ttggccgcaa agcgccggag gttggcggca
    11761 aactgcgcgt cgttcgggtc caggttgccg acgagaccga cgatggattt ttccttttcc
    11821 gccaggtcga gcacccattg attgtcttcg acccattggc tggcctcgac gacgacggtc
    11881 tcgcgcgcgc cgtggggcgc ggccacggcc agccagtcgg cgggcagcac ggtgcggtgc
    11941 agcggcgagt ttttcggcgg ccagggcacg ccctgggggc gcgcgggatc ataaaaatgc
    12001 gtgtgggtgt caatgacgcg cgtggcggca ggcgcggcgc gctgggtgga ggggagcagc
    12061 gcggctgcgg cggtggcttg gagaagtcgg cggcggttca tgcggggtgg agttaacggc
    12121 gcgcatggcg cggctttgca ggcgcggcca caggcgcgct tgtgcgcggg ccaaagcgtt
    12181 tcattcttgc cgctgtcacc gctccggatg ttcgacccct tcaccaccct ccaggaaacc
    12241 ggcttctcac agggcgcgga ctccgcgcgc ccgggcggct ggacgccgga gctgatcgcc
    12301 gcgctggact ggatgcgcgt ggcggagctg gcgcgcgcca tcgccgggca caacggctgc
    12361 gagctggccg gctcgcgcgt gctggaggat gcctccgtgg tcttcgccat gctggagcgt
    12421 cccaccaccg cgcatcccca gcgcgctctg gtgaaaaccg cgggctggaa tgaatggggc
    12481 gccaccccgg agagcgtgcg ccgtttttcc accgaggtgg ccacggcgaa aaacacgcgc
    12541 ggcatcctca tcgcgccggg aggcttcagt cccgccgcgc ttctggccgc gcaagccgcg
    12601 cgcatcgagg cggtggatgc cgcggcgctg cacgcggtgc tcatgaggct gcccgccgag
    12661 cgcagtgatt tctttttcac catagccacc gccggggact gccgccggcc cagctgcccc
    12721 gtctgcctca ggaaactcga gcgctccggg aaggatgacg ccgccgcgca ggacgagcac
    12781 cggaccatcc gcgccgaggg cctggtggcc gatcccgtgg agtgcggcac gctgagtgtt
    12841 cccgccggcg ccgaggtgac ctttctctac gaggtgcgcg cgcgccgtgt ccacatcgcc
    12901 gggcatgcgc agggcgactt cgcctgcgag ggcaccgtca tcatcgagcc gggcggcatc
    12961 ctcaccggca cggtgacggc gcgcgcagtc gaagtgcgcg agggcggcga gctgcgcggg
    13021 cagttccgca tcctggaggg agcgctgcgg ccc
//
LOCUS       DAOMYJ010000148        12886 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Prosthecobacter sp. isolate AMR_MDS_5629
            NODE_1626_length_12886_cov_18.196477, whole genome shotgun
            sequence.
ACCESSION   DAOMYJ010000148 DAOMYJ010000000
VERSION     DAOMYJ010000148.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867850
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prosthecobacter sp. (wastewater metagenome)
  ORGANISM  Prosthecobacter sp.
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Verrucomicrobiaceae; Prosthecobacter.
REFERENCE   1  (bases 1 to 12886)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12886)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.30
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:34:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,742
            CDSs (total)                      :: 4,684
            Genes (coding)                    :: 4,676
            CDSs (with protein)               :: 4,676
            Genes (RNA)                       :: 58
            tRNAs                             :: 55
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12886
                     /organism="Prosthecobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1626_length_12886_cov_18.196477"
                     /isolate="AMR_MDS_5629"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1965333"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..192
                     /locus_tag="PLP58_14690"
     CDS             <1..192
                     /locus_tag="PLP58_14690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ09822.1"
                     /translation="SNQTHFFANPIHFSAKPTCFSAKPTCFSRNPICFSAKPIHFFVN
                     QTCFFGNLSCFSAKPSCFS"
     gene            666..1436
                     /locus_tag="PLP58_14695"
     CDS             666..1436
                     /locus_tag="PLP58_14695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ09823.1"
                     /translation="MADTFHTKAGNDYRDALTEWLQNTRLISEAVYRLYGDYQKDGRV
                     VFSDPSWPKPKSQGEFPLSQFTIQPIPLGVRMCDLLKSSWSAQFVQLESLWEQYLQDV
                     VLELRHKDVAIFEPFCEREYMASLVRDVLMGSIEDIEEIKNEAATRFATGITRLAWRD
                     QWKQLKRLEVGLTDAAEKEPWFSQLDTYFEMRNCVIHRASRPSQLLHQKTSYYSSKNI
                     QVIEIWPQHLDYYRKQFIACLAYVEERIKAKYTKQKQR"
     gene            complement(1687..2499)
                     /locus_tag="PLP58_14700"
     CDS             complement(1687..2499)
                     /locus_tag="PLP58_14700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ09824.1"
                     /translation="MTPAQAYDAILQPARPPLRDRSEEAARRALECAEALAGSVQGES
                     FGWHRRQKREGLLDDQLALLRHFARQDTAPGEPIGQGGEHEVWHTENGTHVSKFTIHN
                     QFGYVVDQENDNRANKLRLRPALPSEYLMRLGTQNAVFGDAITLQGIRAGSIPSIITA
                     QPYADQGRPSQADVDAFLWQSGFIRLPDEMMMGQFSHKPFWWRPAGSILVGDSNPENY
                     SRISDDIIVPIDVISHPFPRSLIEQTARQNGVSLHHLVAQDARQREVFDHRQ"
     gene            complement(2871..4268)
                     /locus_tag="PLP58_14705"
     CDS             complement(2871..4268)
                     /locus_tag="PLP58_14705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009964087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HRJ09825.1"
                     /translation="MSTETQPTRREFLGKTAAAATVTSVTSVAATGLTVAQSANVAGS
                     DRIKVGLIGSGGRGSGAANQALSTKQEGVVLHAICDAFKEKADGALSNLRTKHADKVQ
                     VDDSRIFTGLDGYKGVIDSCDLVILTTPPGFRPYHFEAAVNAGKHVFMEKPVAVDSPG
                     VRKVLEMAKVADEKNLKVVVGLQRRYQNCYLEALKQVKEQGIIGDIVSGQVYWNGGGI
                     WFRDKQEGQSELMYQINNWYFFTWLCGDHINEQHIHNIDVANWFIGGHPESAQGMGGR
                     MQRVEKKYGQIYDHHYVEFTYANGVRVNSQCRHQAGVYNAVREEFTGTKGKLYLDNGA
                     QKTYALDHKGNTIWRYRPARAAADAKGKGKRVVTGDPDPYQTEHDILQAAIRGNTPKN
                     DAYYGAESTLSAVMGRMATYSGKEIKWDEALNSKVQHMPAIVTAETEPPAKPDGEGGY
                     PVAIPGVQVPGAEVI"
     gene            complement(4308..5162)
                     /locus_tag="PLP58_14710"
     CDS             complement(4308..5162)
                     /locus_tag="PLP58_14710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009964088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HRJ09826.1"
                     /translation="MKIGFNLLLWTGHVTDENFPVLEKLKAAGYDGVEIPIFDVSNPA
                     HFAGIGRVLKDNGLECTAVTVLPDEAHNAISPVAANRQGAIDHLKRVLECAHNAGVQT
                     LCGPYYQVLGQFTGKYPSEEELSGAAEVHRAIAPVAEAAGVKCAIEALNRFEAHLLNT
                     MEQAAAYVKRVGHPNFGTMYDTFHANIEEKNPITAIETVFATGKLNHVHISENDRGTP
                     GRGHINITGQIQHLKKIGYDGWMTIEAFGGALPDLAAATRVWRDFFPSTEQVYLEGIE
                     VIRKALAA"
     gene            5353..5856
                     /locus_tag="PLP58_14715"
     CDS             5353..5856
                     /locus_tag="PLP58_14715"
                     /inference="COORDINATES: protein motif:HMM:NF013630.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HRJ09827.1"
                     /translation="MLKTARPYLVLLPLVMLAACENMQKAPSNYDPYVSNYGNDGGYN
                     PYPGQPGYAQSGGAPAAPSYDLPPAPVEADPYAFSTSGSTTPKPSGASASTPKKPATT
                     ASKSSTSSKSKAAPKKAVTSGTHTVVKGDTLYGIALKRKTTVAKIKAANGLKSDLIRP
                     GQKLKIP"
     gene            5940..6614
                     /locus_tag="PLP58_14720"
     CDS             5940..6614
                     /locus_tag="PLP58_14720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ09828.1"
                     /translation="MQAPPSSPPSAAVSSSPVRRQTSWRRKVLIVLGVLALGCGIAAA
                     ASAWWVKRNLYASPMRPVSLSVAEQTDLDAKLKALESSPAPAAPSAEQERTLVITARE
                     INAYLAGQGLGETFKVDLGRDSIAVTTIAPIPEDSGLPLLAGATLRVRLSLTLAMDPG
                     QKLRLAVNDVRVGCVPLPNAWLGDIKGVNLVSEGVEGDPAMQRFFAGIQSAEVAPDGI
                     RIILNE"
     gene            6781..7716
                     /locus_tag="PLP58_14725"
     CDS             6781..7716
                     /locus_tag="PLP58_14725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ09829.1"
                     /translation="MKLKSMTSVITKSALVLATCASLNAFAGTSAPAKGYVAPQPEPD
                     GALFDTLGASVEVGYDTRYYFRGLYRADNTVSAGINLSVPLTEQVSLGLGARYISTVE
                     TTAATPGPFGQGDFDFSELALSTGLNYDAGFATLGLVFNHYEYFDGFSGTNGGIQAPN
                     SGEFNVSSAQEVGLTAATSLGPVNVYAGWYYDFRIGGSYAEVGADLPIEITSWLSIVP
                     GFRTGYGFDYYTLPQNGSRAGGWTHVIPTISMPIKLTKSATLTPYVAYNISLDSRHDA
                     AKGSSTGLAGAFPTGINANDQDTEFFGGVRLSVSF"
     gene            7958..8857
                     /locus_tag="PLP58_14730"
     CDS             7958..8857
                     /locus_tag="PLP58_14730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ09830.1"
                     /translation="MKLKSMTSVITKSAMVLAACASLSAFAGPVAPSGKGTVAPLPEP
                     DGALFDTLGAELAVGYDTRTYYRGLWFADNAVWTSLNLSIPLTEQVSLGLGALYLSTV
                     ETTMDAVNGPGVPNNGVEDFDYSELDLMASLSYDAGFATFGLVYTHYEFWDGYSGTNF
                     GLPNGAGEGNVSGADEVGITFATSVGPVNLAGGYFYDFRIGGSYFELGGDLPIEITSW
                     LSLVPAVKVGYGIDYYTSGVNPAAPPSGFTHVLPTLSAPIKLTKSATLTPYIAYNISL
                     GARHALNTQDSEIFGGVKLGVTF"
     gene            8960..10642
                     /locus_tag="PLP58_14735"
     CDS             8960..10642
                     /locus_tag="PLP58_14735"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: protein motif:HMM:NF013113.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HRJ09831.1"
                     /translation="MATPLNWNSSSLTRGWQRGVNAFFWGLGFKPEDFDKAQIGIGTP
                     LLDGNICNIHAHELARLIKQGCDEAGLIGFPFGVSPVSDNITQGNIGGAASLCSRNLM
                     ANGAEMVCTSHCYDALIGLHHCDKNGPAFAMALARTNYPGLIVNGGSIMPGCHDGRPT
                     SILDVYDAAAREKQGTMSYEESEQIIRTACPGAGGCGIAASFNTWGIALEAMGLSLPD
                     TSSIPAIDSAKRAECLRVGRAVRLLLERGIRPRDIITKASLSNAMTAIAAAGGSTNGV
                     LHILAVAREAGVDFTLRDVQAICRRTPVLCNFAPRGRGTMNDLHRLGGTTMFLKHLHG
                     TGLLDGSCLTVTGATLAENLAGAPPVPFPNELIAPLDTPFKAYADIQICFGNISPHGM
                     VFKVSSLDEPCFTGTAICFTDSKGVSDAAAAGRIRPGHIVVIRGCGPVALGMPEMHVA
                     SAALAVPELYGKVALLSDTRVSGVSGGAVGVHCSPEAAVGGPIARVRDGDEIEFDLLA
                     GTIEVKADLDARGGGAPPGPPRHPFGYLADFAATVTQAHEGCVPAWVLQKRQ"
     gene            10700..11728
                     /locus_tag="PLP58_14740"
     CDS             10700..11728
                     /locus_tag="PLP58_14740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ09832.1"
                     /translation="MNSAWSIRLVRALFFALFVFTGITIALGFQQPAWKGALTGAAVM
                     GLLLALDALFARFTLRDFSHATFGLAIGLFCAWLITRIGVFQLAYFQTLPEGDTMQNV
                     VELLIYASMAFFGVTFALRSDRDQFAFLIPYVRFRRDASEGEPLLLDAGIIVDGRIPE
                     VAQTGFLGGMMVVPRFVLEELRHLSESADSLQSARGRRGLEVMERLRALGGQRLSIHE
                     DPPAAAASRETEDARLVTLARELNARLLTNDTSLAKVARLRGIIVLSFQDLSLALQPQ
                     FAPGEELQLTLVKPGKDKHQAVGYLPDGTMIVVNQAASFIGQTVDVVISGTLPTSAGR
                     LIFAEMKR"
     gene            complement(11731..12690)
                     /locus_tag="PLP58_14745"
     CDS             complement(11731..12690)
                     /locus_tag="PLP58_14745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ09833.1"
                     /translation="MRLRDPWLARSLACLLLAGQARGIVLYDTDDPGANTVAPAGIYE
                     SAGWGWQGLFGSFLGTMIGPRHFVTAQHIGPQGGTFISSALFNGVADAAYHVDATANG
                     GAGYWDIAGTDLRVYQIQETFSSYAPLYDGADETGLEMIVFGRGGPRGAEVTLGGDLK
                     GWLHTAADGTPRWGLNTVDGIANTGFGPLLMAGFDAVAGVDEGTLSAGDSGGAVFVLD
                     GGVWKLAGINFSVDGLFDTNAVTGDGGEFAAALFDKGGLYQGSDGGGWSLVADTAEDA
                     PSMMYASRIAPNVAEIQSITLVPEPAGAFLLMLAGLAGLGRRR"
BASE COUNT         2297 a         4223 c         3814 g         2552 t
ORIGIN      
        1 agcaaccaga ctcatttttt tgccaaccca attcattttt ctgccaagcc gacttgtttt
       61 tctgccaagc cgacttgttt ttctcgtaac ccgatttgtt tttctgccaa acccattcat
      121 ttttttgtca accaaacttg tttttttggc aacctgagtt gtttttctgc caaaccgagt
      181 tgtttttctt gaagacccca tggttttgca agcggtccaa tctggcaaca acgcagcccc
      241 aggtagggcg gctggtcccc cagccgccgc gcgcgcaagt gcaggctacg agcggcgctg
      301 acaggtcatt cgcaccctca gcccgcgcgc gcggcgcgtt gaggaaagag cgacatgcca
      361 agccccttca ccgccttggc cgtgcccctc cgccccttgg aagagacgat ccccacgctg
      421 ccccaccaca cttcccgaag gtcaagaacc ccgaaccaac ccgatattct gtgtacctat
      481 cagttccttg gaaatcctga atgatgaagc aagcccaaac atcccaaaag ccgaagcaaa
      541 tgcccaaaaa ccttgtcatt caaggcctcc agccgcatca tcgcccgaaa atgaacccca
      601 aaatatcgcc gccaagcgat atcgcattac actaacaaca ctgttagccc aaccaacgga
      661 atccaatggc agacaccttc cacaccaaag cgggaaacga ttacagagac gcactcactg
      721 agtggctcca gaatacccgc ttaatcagcg aggccgtcta tcgactttac ggggactatc
      781 agaaggacgg gagagtcgtc ttcagtgatc cgtcgtggcc taagcccaag agccaaggtg
      841 agtttccgct ttcacagttc actatccagc cgatacctct cggggtgagg atgtgtgatc
      901 tattgaagtc ttcatggtct gcgcagttcg tccagctaga gtcactttgg gaacagtatt
      961 tgcaggatgt tgttttggag ttgcgccaca aagatgtggc catctttgaa ccattttgtg
     1021 agcgagagta tatggcttcg ctggttcgtg atgttctgat gggatcgatt gaggacatcg
     1081 aggagatcaa gaacgaggcg gccacgcgct tcgccacggg cataacccgc ctcgcttggc
     1141 gagatcagtg gaagcaactc aagcgccttg aagttggtct cactgatgca gcggagaagg
     1201 agccttggtt ctcacagttg gatacatatt ttgagatgcg gaactgtgtg attcatcgcg
     1261 ctagcaggcc atcacagcta ttgcaccaga agaccagcta ctattcctcg aagaatattc
     1321 aggtgatcga gatttggccc cagcatctcg attactatcg caagcagttc atagcctgcc
     1381 ttgcatacgt tgaagaacgc atcaaagcaa aatacaccaa acaaaaacag cgctaacaat
     1441 ggcgctgctg gacaacggga ggggcgcttg ttgtcgtatc agagcccacc acaatttgaa
     1501 catcacccac ttcgttcatg ctctccgccg cccctcccgt gccagagctt gatcgttagg
     1561 ccaagaatgg aactgtgaac cagaccgtaa gccgccttcg ccgtcgggct tggcgggggc
     1621 tcggtgactt ggccttcgtg tgcccgcaga ccaccgcctt gcggcggcgg ccacattcag
     1681 cggcggctac tggcggtggt caaagacttc gcgctgccgg gcgtcctgtg ccacgaggtg
     1741 gtgcaggctg acgccgtttt ggcgggcggt ctgctcgatc aggctgcggg ggaaggggtg
     1801 ggaaatgaca tctatcggca cgatgatgtc gtcggaaatg cggctgtagt tctcgggatt
     1861 ggagtcgccg acgaggatgc tgccggcggg ccgccaccag aagggcttgt gggagaactg
     1921 ccccatcatc atctcatcgg gcaggcggat gaagccggac tgccagagga aggcgtccac
     1981 gtccgcctgg gaagggcggc cctggtccgc gtaaggctgg gcggtgatga tggaggggat
     2041 gctgccggcg cggatgccct ggagggtgat ggcatctcca aacacggcgt tctgcgtgcc
     2101 gaggcgcatg aggtattcag agggcagggc ggggcgcagg cggagcttgt tggcgcggtt
     2161 gtcgttttcc tggtcaacga cgtagccgaa ctggttgtgg atggtgaact tggagacgtg
     2221 ggtcccgttc tcggtgtgcc acacctcatg ctcgcccccc tggccgatgg gttctccggg
     2281 cgcggtgtcc tgccgcgcga agtgccgcag gagggctagc tggtcatcga gaagcccctc
     2341 gcgtttctgg cggcgatgcc agccgaaact ctcgccctgg acactgccag cgagggcttc
     2401 ggcgcattca agcgcgcggc gagcagcttc ttcggatcgg tcacgaagcg gcgggcgggc
     2461 gggctggagg atggcgtcat aggcttgggc gggtgtcacc ttgggcggaa gggtaaaggc
     2521 tggctccagg ctgtcaacgc ccgtcgcagc ggcgtgtgct gtggctgcgg gcgcgagccc
     2581 gtggcatccg aatgtctcgc ggccttcgtg cgctcccaag ccgtaggttg ggaatgtccg
     2641 gcgtatgaga cgtggtttgc agtttgatga tcttcgccgg acttcccgac tgggttcgag
     2701 cgtctcttgg ccttcgcacg ccctcaaacc gtcgggaagt ttggcgatct cgtcttgtga
     2761 gatagcggag ttttctccgc caaacattcc caacctactt ttgcaccgcc caacaaaaaa
     2821 ccgccggaca tgacgggcat gtccggcggt gtggattgct gaatgaaggc tcagatgact
     2881 tcggctccgg ggacctggac gccggggatg gcgacggggt agccgccttc gccgtcgggc
     2941 ttggcggggg gctcggtctc ggcggtgacg atggcgggca tgtgctggac tttggagttc
     3001 agggcttcgt cccatttgat ctccttgccg gagtaggtgg ccatgcggcc catgacggcg
     3061 ctgagggtgg actcggctcc gtagtaggcg tcgtttttcg gggtgttgcc gcggatggcg
     3121 gcctggagga tgtcgtgctc ggtctggtag gggtcggggt cgcccgtgac gacgcgtttg
     3181 cctttgcctt tggcatcggc ggcggcgcgg gcgggccggt accgccagat ggtgttgcct
     3241 ttgtgatcga gggcgtaagt tttttgagcg ccgttgtcga ggtagagctt gcctttggtg
     3301 ccggtgaact cttcgcgcac ggcattgtaa acgccggcct ggtggcggca ctggctgttg
     3361 acgcggacgc cgttggcgta ggtgaactcg acgtagtggt ggtcgtagat ctggccgtat
     3421 tttttttcca cgcgctgcat gcgtccgccc atgccctggg cgctctcggg gtggccgccg
     3481 atgaaccagt tggcgacgtc gatgttgtgg atgtgctgct cgttgatgtg gtcgccgcag
     3541 agccaggtga agaagtacca gttgttgatc tggtacatga gctcggactg gccttcctgc
     3601 ttgtcacgga accagatgcc gccgccgttc caatagacct ggccggagac gatgtcgccg
     3661 atgatgccct gctctttgac ctgcttgagg gcctcgaggt agcagttctg gtagcggcgc
     3721 tggaggccga cgacgacttt gaggtttttc tcgtcggcga ctttggccat ttcgaggact
     3781 ttgcggacgc cggggctgtc cacggcgacg ggtttttcca tgaagacgtg cttgccggcg
     3841 ttgacggcgg cttcgaagtg gtaggggcgg aagccgggag gggtggtgag gatgacgagg
     3901 tcgcaggagt ctatgacgcc tttgtagccg tcgagcccgg tgaagatgcg ggagtcgtcc
     3961 acctggactt tgtcggcgtg cttggtgcgg aggttggaga gggcgccgtc ggctttttct
     4021 ttgaaggcat cacagatggc gtggaggacg acgccttctt gcttggtgct gagggcttgg
     4081 ttcgctgcgc cggagccgcg gccgccggag ccgatgaggc cgactttgat gcggtcgctg
     4141 ccggcgacgt tggcggactg ggcgacggtg aggccggtgg cggcgacgga ggtgacggag
     4201 gtgacggtgg ctgcggcggc ggttttgccg aggaattcgc ggcgggtggg ttgggtttcg
     4261 gtgctcatgg ggatggggct gggtgtgtga agtgtggggt gggtggctca ggcggcgagg
     4321 gctttgcgga tgacttcgat gccttcgaga tagacctgct cggtggaggg gaagaagtcg
     4381 cgccagacgc gggtggcggc ggcgaggtcg gggagggcgc cgccgaaggc ttcgatggtc
     4441 atccagccgt cgtagccgat ttttttgagg tgctggattt gcccggtgat gttgatgtgg
     4501 ccgcgcccgg gtgtgccgcg gtcgttttcg gagatgtgga cgtgattgag cttgccggtg
     4561 gcgaagacgg tctcgatggc ggtgatgggg ttcttttcct cgatgttggc gtggaaggtg
     4621 tcgtacatgg tgccgaagtt cgggtggccg acgcgcttca cataggcggc ggcctgctcc
     4681 atggtgttga ggaggtgggc ttcgaagcgg ttgagggcct cgatggcgca cttgacgccg
     4741 gcggcctcgg ccacgggggc gatggcgcgg tggacttcgg cggcgccgct gagctcttct
     4801 tcggaggggt atttgccggt gaactggccg agcacttgat aataggggcc gcagagggtc
     4861 tgcacgccgg cgttgtgggc gcattcgagc acgcgcttga ggtggtcaat ggcgccctgg
     4921 cggttggcgg cgacggggct gatggcgttg tgcgcttcat cgggcaggac ggtgacggcg
     4981 gtgcattcga ggccgttgtc cttgagcacg cgaccgatgc cggcaaagtg ggcgggattg
     5041 ctcacgtcga agatggggat ctcgacgccg tcgtagcctg cggctttgag tttttcgagc
     5101 acggggaagt tttcatcggt cacatgaccg gtccagagga ggaggttgaa gccaattttc
     5161 atgaaatagg aagcgcagac tagagatgcc gcgcgggaat cgcaagactt ggttccaacg
     5221 aaatcctgcg cgctgttttt catggcttgc gccacgggcg gagcattcag ccgccttgtg
     5281 ttggggcatt ttgccttgat caaggcgggc gtgaatccat acttggccgg atttcccact
     5341 tctcctctac ccatgctgaa aaccgcccgc ccgtatctgg tcctcctgcc cctggtcatg
     5401 ctcgccgcct gcgagaacat gcaaaaagcg ccctcgaatt acgatccata cgtctcgaac
     5461 tacggcaacg acggcggcta caatccttat cccggccagc cgggctacgc ccaaagcggg
     5521 ggcgcccctg cggcaccgag ctatgacctg ccgcccgcgc cggtagaagc cgacccttac
     5581 gctttcagca cttccggatc cacgacaccg aagccgtcgg gcgcctccgc cagcacgccg
     5641 aaaaagcccg ccaccacggc gtcgaagtcc tccacctcgt cgaaatccaa ggccgctccc
     5701 aaaaaagccg tcaccagcgg cactcacacc gtggtcaagg gggacaccct ctacggcatc
     5761 gccctcaagc gtaaaaccac cgtcgccaaa atcaaagccg ccaacggttt gaaatccgac
     5821 ctcatccgcc ccggccagaa gctgaaaatc ccctgatctg gcgctgcaaa aaaagccgga
     5881 atttggagga acaggaggct cgaacaaagg gtagatgagc gcgtctcata ctcatcgtca
     5941 tgcaagcacc accctcctct ccgccatcag ccgcagtcag ctcgtcaccc gtccgccgcc
     6001 agacatcctg gagacggaag gtgctcatcg tcctcggtgt tctcgctctg ggctgcggca
     6061 tcgcagccgc cgccagcgct tggtgggtga agcgcaatct ttacgcttca cccatgcgcc
     6121 ccgtctcact cagtgtggcg gagcagacag atctggacgc caaacttaaa gcgctggagt
     6181 cctcccccgc accagcggct ccatccgccg agcaggaacg caccctggtg attacagcgc
     6241 gcgaaatcaa cgcttatctg gcaggccagg gactcggaga gaccttcaaa gtggacctgg
     6301 gacgcgacag catcgccgtc actaccatcg ctcccattcc tgaggacagt ggtttgcccc
     6361 tcttggcggg cgccaccctg cgcgtgcgcc tttccttgac cctggcgatg gatcccggcc
     6421 aaaaactgcg cctcgccgtc aatgatgtgc gggtgggctg cgtgcccctg cccaatgcct
     6481 ggctgggtga catcaaaggc gtgaatctcg tcagtgaagg cgtggaaggc gatccggcta
     6541 tgcagcgctt tttcgccggg attcaaagtg ccgaggtagc ccctgacggc atccggatca
     6601 tcttgaacga gtagccctcc cagggggcca gaacggatga gatgcggatt gttaataacc
     6661 cggtaattta tggaaaatcg cgcttggcaa tcaatgagtt gtcatctagc gtgatccgat
     6721 tggtggaagg tctcccggac caacctccat caaccaccaa ccaaaccaac cgaaccaacc
     6781 atgaaattga aaagcatgac ctccgtcatc acgaagtcgg cgctcgtcct ggccacctgc
     6841 gccagcttga acgccttcgc cggaacatcc gcccctgcca aggggtacgt tgctccgcaa
     6901 cccgaacccg atggtgctct ctttgacacc cttggcgctt ctgtggaagt gggctatgac
     6961 acccgctact acttccgcgg tctctaccgt gctgacaaca ccgtcagcgc cggcatcaac
     7021 ctcagcgtgc cgctcaccga gcaggtttcg ctgggtcttg gcgcccgcta catcagcact
     7081 gtcgagacaa ccgcagccac tcctggcccg ttcggtcagg gagatttcga tttttccgaa
     7141 ctcgccctgt ccaccggatt gaactatgac gccggtttcg cgacccttgg cctcgtgttc
     7201 aaccactacg agtacttcga cggcttctcc ggcaccaacg gtggaatcca ggcgcctaat
     7261 tccggtgaat tcaatgtcag cagcgctcag gaagtgggtc tcaccgcagc cacaagcctc
     7321 ggtcctgtga atgtgtacgc gggctggtat tacgacttcc gcatcggcgg ttcctatgcc
     7381 gaagtcggag ccgacctgcc tatcgaaatc acctcctggc tgagcatcgt ccccggcttc
     7441 cgtacgggct atggattcga ctactacaca ctgcctcaaa acggctctcg tgctggtggc
     7501 tggacccatg tcattccgac catttcgatg ccgatcaagc tgaccaagag cgccactctg
     7561 acgccttatg tcgcttacaa catctccctc gacagccggc atgacgctgc caaaggaagc
     7621 agcaccggcc tcgctggagc gtttcccaca ggcatcaacg ccaacgacca ggacaccgag
     7681 ttcttcggcg gcgtgcgtct gagcgtcagc ttctaattca cgtcggaact ctcgaaagag
     7741 aaattccttc attcctgact cccggccagt gctcctggcc gggagttttt tgtttgccgg
     7801 cagtgcatga cagacgctgg agaaagggcg gaaaatattc tgaaattatt ggaattggca
     7861 cccggtgttg gcacgcgaaa tgcttttctt aaatcggtgg tggctggtct cccggacctc
     7921 catccaccac atcaacaaac caaatacacg acccaacatg aaactgaaaa gcatgacctc
     7981 cgtcatcacg aaatcagcca tggttctcgc cgcctgcgcc agcctcagcg ccttcgccgg
     8041 ccctgtcgcc cccagcggca aaggaaccgt ggcccctctg cccgaacccg atggtgctct
     8101 ctttgacacc ctgggcgcgg aactcgccgt cggctacgac acccgcacct actaccgcgg
     8161 cctgtggttc gccgacaacg ccgtctggac cagcctgaat ctgagcatcc cgcttaccga
     8221 gcaggtttcg cttggccttg gcgctctcta cttgagcacg gttgaaacta ccatggacgc
     8281 tgtcaatgga cccggtgtgc cgaacaatgg tgtcgaagac ttcgactact ccgaactcga
     8341 cctcatggcc agcctcagct atgacgccgg cttcgccacc tttggcctgg tttacactca
     8401 ctatgagttc tgggatggct attccggcac caacttcggt cttccgaacg gtgctggcga
     8461 aggcaacgtc tccggcgccg acgaagtggg catcactttt gccacctccg ttggtccggt
     8521 gaatcttgcc ggcggttatt tctatgactt ccgcatcggc ggctcctact tcgagcttgg
     8581 cggagacctc cccatcgaaa tcacctcctg gctgagcctc gtgcccgccg tcaaagttgg
     8641 ctacggcatt gattactaca ccagcggtgt caatcctgcg gctcctccca gcggcttcac
     8701 tcatgtgctg ccgaccctct cggctccgat caagctgacc aagagcgcca ccctgacccc
     8761 ctacatcgcc tacaacatct ccctcggtgc gcgccacgcg ctcaacaccc aggatagcga
     8821 gatcttcggc ggcgtgaagc tcggcgtgac cttctaatct gacagtttcc cgcgaaactg
     8881 cctttccgcc cccggatgct tcagcgtccg ggggtttttg tttggatgtg gagcgatttg
     8941 cccgctatat gcggccagca tggcaactcc cctgaattgg aactcaagct ccctcacccg
     9001 cggctggcag cgcggcgtca acgccttctt ctggggcctc ggattcaagc ccgaggactt
     9061 cgacaaagcg cagatcggca taggcacccc gctgcttgat ggcaacatct gcaacatcca
     9121 cgcccatgag ctcgcccggc tcatcaagca gggctgcgac gaggccggcc tcatcggctt
     9181 tcccttcgga gtctccccag tcagtgacaa catcacccag ggcaacatcg gcggcgcggc
     9241 ctccctttgc tcccgcaatc tgatggcgaa cggcgccgag atggtctgca cctcgcattg
     9301 ttacgacgcc ctcatcggac tgcatcattg cgacaaaaac ggccccgctt ttgccatggc
     9361 cctggcgcgc acgaactacc ccggcctcat cgtcaacggc ggcagcatca tgcccggctg
     9421 ccacgatggc cggcccacca gcatccttga tgtctatgac gcagccgcca gggaaaagca
     9481 gggcaccatg agctacgagg agtccgagca aatcatccgc accgcctgcc ctggcgcggg
     9541 cggctgcggc atcgccgcct ccttcaacac ctggggcata gcccttgagg ccatgggcct
     9601 cagtctgccg gacacctcct ccatccccgc catagactcc gccaagcgcg ccgagtgtct
     9661 gcgcgtcgga cgcgccgtgc gcctgctcct ggagcgcggt attcgtccgc gcgacatcat
     9721 caccaaagcc tccctctcca acgccatgac cgccattgct gccgcaggcg gctccaccaa
     9781 tggcgtcctg cacatcctcg ccgtcgcccg cgaggccggc gtggacttca ccctgcgcga
     9841 tgtccaggcc atctgccgcc gcacccccgt gctgtgcaac ttcgcacccc gcggacgcgg
     9901 caccatgaac gaccttcacc gcctcggcgg caccaccatg ttcctgaagc acctccacgg
     9961 caccggcctg ctcgacggct cctgcctcac cgtcaccggc gccaccctgg cggaaaacct
    10021 cgccggcgcg ccgcccgtgc ctttccccaa cgaactcatc gcccccctcg acactccctt
    10081 caaagcctac gccgacatcc agatctgctt cggcaacatc tccccgcacg gcatggtctt
    10141 caaagtctcc tccctcgacg agccctgctt caccggcacc gccatctgct tcaccgacag
    10201 caaaggagtc tccgatgccg ccgctgcggg caggatccgc cccggtcaca tcgttgtcat
    10261 ccgcggctgc ggtcccgtgg cactcggcat gcccgagatg catgtggcca gtgccgcgct
    10321 cgccgtgccc gagctttatg gaaaagtggc cctgctctcc gacacccgcg tttctggtgt
    10381 ctccggcgga gccgtcggcg tgcattgcag ccccgaggcc gccgtcggcg gccccatcgc
    10441 ccgcgtgcgg gatggcgacg agatcgagtt cgacctgctc gccggcacca tcgaggtcaa
    10501 agccgatctc gacgcccgtg gtggcggcgc cccgcccggg ccgccgcgcc atcccttcgg
    10561 atacctggcc gacttcgccg ccaccgtcac ccaggcgcat gagggctgcg tgcccgcgtg
    10621 ggttttgcaa aaacggcagt gagcgtcttt tgtctcgtca gccagcgtcc cgccggttaa
    10681 aagtccggcc aagcatctca tgaactccgc atggtccatc cgcctggtca gggcgctgtt
    10741 tttcgcgctc tttgtcttca ccggcatcac catcgccctc ggcttccagc agccggcctg
    10801 gaaaggcgcg ctcacaggcg ccgccgtcat ggggctgctg ctcgcgctcg acgccctctt
    10861 cgcccgcttc accctgcgcg acttctccca cgccaccttc ggccttgcca tcggcctctt
    10921 ttgcgcctgg ctcatcaccc gcatcggcgt cttccagctc gcctacttcc agaccctccc
    10981 cgagggcgac accatgcaga atgtcgtcga gctgctcatc tacgcctcca tggccttctt
    11041 cggcgtcacc ttcgccttgc gcagcgaccg cgaccagttc gccttcctca tcccctatgt
    11101 gcgcttccgc cgcgacgcct ccgagggcga gcccctcctg ctcgatgccg gcatcatcgt
    11161 ggacggccgc atccccgagg tggcccagac cggcttcctc ggcggcatga tggtcgtccc
    11221 gcgcttcgtc ctagaggagt tgcgccacct ttccgagtcc gccgacagcc tccagtccgc
    11281 gcgcgggcgg cgcggactcg aagtcatgga aagactccgc gccctcggcg gccagcgtct
    11341 ttccattcat gaggacccgc ccgcagccgc cgcctccagg gagaccgagg acgcccgcct
    11401 cgtcaccctc gcccgcgagc tcaacgcccg cctcctgacc aacgacacca gcctcgccaa
    11461 agtcgcccgc ctgcgcggca tcatcgtcct cagctttcag gacctctccc tcgccctcca
    11521 gccgcaattt gcccccggag aggagctcca gctcaccctc gtcaagcccg gcaaggacaa
    11581 gcaccaggcc gtcggctacc tgcccgacgg caccatgatc gtcgtcaacc aggccgcctc
    11641 cttcatcggc cagaccgtgg atgtcgtcat ctccggcacc ctgcccacca gcgccgggcg
    11701 cctcatcttc gccgagatga agcgctgatc tcagcgcctc cgccccagcc ccgccagccc
    11761 cgccagcatc aggagaaaag cccccgccgg ctccggcacc agcgtgatgc tctgaatctc
    11821 cgccacattc ggcgcgatgc gcgaggcgta catcatcgag ggggcatcct ccgccgtgtc
    11881 cgccaccagg ctccagccgc cgccgtcgct gccctggtaa aggccgccct tgtcaaacag
    11941 cgccgccgcg aactcgcccc cgtcacctgt caccgcgttc gtgtcgaaca gcccgtccac
    12001 gctgaagtta atgcccgcca gcttccacac acccccgtcc agcacaaaca ccgccccgcc
    12061 cgagtccccc gccgaaagcg tcccctcatc cacgcccgcc accgcgtcga accccgccat
    12121 cagcagcggc ccgaagcccg tgttcgcgat tccgtccacc gtgttcagcc cccagcgcgg
    12181 cgtgccatcc gccgccgtgt gcagccagcc cttcaagtcc ccgcccagcg tcacctccgc
    12241 cccgcgcggg ccgccgcgtc cgaacacgat catctccagc ccagtctcat ccgccccgtc
    12301 ataaagcggc gcgtaactgc tgaaagtctc ctggatttga taaacccgca ggtccgtgcc
    12361 cgcgatgtcc cagtagcccg cgcccccgtt cgccgtcgca tccacatggt aggccgcatc
    12421 cgccaccccg ttgaaaagcg cgctgctgat gaaggtcccc ccctgcgggc cgatgtgctg
    12481 cgccgtcacg aaatgccgcg gcccgatcat cgtccccagg aacgagccga acagcccctg
    12541 ccagccccag cccgcgctct cataaatccc cgccggagcc accgtgttcg cccccggatc
    12601 atccgtgtca tacagcacga tcccgcgcgc ctgccccgcc agcagcaggc aggccaggga
    12661 ccgggcgagc cagggatcac gcaacctcat gccgaaaaac atatccgctt ccccgctgca
    12721 tgccaagcgt catccggttt ggtgctcttc ccacccctca ggattccgtc tcaaaatccc
    12781 cgcgcgcatg accgcactgc agagggagga tccgttggcg ggttgggcgg ggtcaaagat
    12841 gggaaaaagg gcggtggagg gtggggtaac ggagggggat gtgagg
//
LOCUS       DAOMYJ010000149        12867 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Prosthecobacter sp. isolate AMR_MDS_5629
            NODE_1630_length_12867_cov_19.859038, whole genome shotgun
            sequence.
ACCESSION   DAOMYJ010000149 DAOMYJ010000000
VERSION     DAOMYJ010000149.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867850
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prosthecobacter sp. (wastewater metagenome)
  ORGANISM  Prosthecobacter sp.
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Verrucomicrobiaceae; Prosthecobacter.
REFERENCE   1  (bases 1 to 12867)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12867)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.30
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:34:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,742
            CDSs (total)                      :: 4,684
            Genes (coding)                    :: 4,676
            CDSs (with protein)               :: 4,676
            Genes (RNA)                       :: 58
            tRNAs                             :: 55
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12867
                     /organism="Prosthecobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1630_length_12867_cov_19.859038"
                     /isolate="AMR_MDS_5629"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1965333"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(658..1572)
                     /locus_tag="PLP58_14750"
     CDS             complement(658..1572)
                     /locus_tag="PLP58_14750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009959246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HRJ09834.1"
                     /translation="MKHLLALALLATAVHADIGLISPADAKKLLDHPDPAQRPIVLDT
                     RGGYKDYFRGHLPQAHHINFDTLRGTDQAVPVQYLPDTLTKALLKRAGADKDRLHLVY
                     ATGATLPNDEILSTSMVVYVLEKFGIQNIRVVDGGLAGWVKAGLKPVREYFGNPPGSL
                     PWRGDKSIGVDVKTVRAHAGKPGNLLVDARPLNEFQGNDDVWLRKGHIPGAISFHWAR
                     LMDGANTHQFKSTAQMKRELQSAGITPDKDIICYCGTSREGSLLYFTLKHVLGYPKVR
                     LYEGSWKEYVWLEGQSLPAERGGKAAGK"
     gene            complement(1585..2271)
                     /locus_tag="PLP58_14755"
     CDS             complement(1585..2271)
                     /locus_tag="PLP58_14755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ09835.1"
                     /translation="MKTNRAHILTAALLAAASTAHGTPTGLNNIPTADTVPHRTVAVQ
                     AFSSFGGANQFAANGPGQHSFWLGFKTGWDFGPLDLEWGMDSPIAPDLTGPLLFQTKV
                     NFSPWENGLLALGVANIAFTDHERWSDPFSYAVLAHDFGLLRGHAGYGRQTGGDTLLL
                     GVDRTWKLLDRNFNLNADLVQTRDQRGWLPSVGAKYDLSKHIVLETWTNFPDQGSVSF
                     IAKINFVFTF"
     gene            complement(2268..3212)
                     /locus_tag="PLP58_14760"
     CDS             complement(2268..3212)
                     /locus_tag="PLP58_14760"
                     /inference="COORDINATES: protein motif:HMM:NF013543.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter"
                     /protein_id="HRJ09836.1"
                     /translation="MLILLFSAVLIVAYANGANANFKGVASLFGSGTASYRTAVLWAA
                     VTTAAGCVAATLLTGAMLKAFSGKDLVPDEMAAQPVFLAAVAAAAGLAGMLATRLGFP
                     VSTTHMLTGALLGAGWAAGDVHVGKLWESFVKPLLLSPVLAVAAGGGVYLVLKTLRLA
                     PDHRTRALDALHFLSAGAVCFARGLNDTPKMAALLAGIGWLQGSAGMMLIAAAMALGG
                     LISARQVAETLAHKITGMNPGQGFAANLATAVLVTTASLHALPVSTTHVSVGALLGIG
                     ITTRQAKWRTALPVLAAWVVTLPVSALLAALIFRLLSP"
     gene            3458..4732
                     /locus_tag="PLP58_14765"
     CDS             3458..4732
                     /locus_tag="PLP58_14765"
                     /inference="COORDINATES: protein motif:HMM:NF024765.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alginate export family protein"
                     /protein_id="HRJ09837.1"
                     /translation="MKTLAAASDRISESPAYAHALAGSLEWLDFGIESRTRYEQRWND
                     YATPGLLSDDALVTRNLLWLGITEKLDPLRLFVEAEDSRRFFSDRTADPNIETGLELI
                     QAFAQLYWEDALAGLPLRINAGRMAFDFVDRRLVARNRNRNTISAFDGLRVRLGEDAT
                     RWELDAFALHPVERIFDARDQAAENIWLAGLAAHWRGSSPHFIAEPYWLWLNQDNDRT
                     TPMRRDIHTLGFHAFGRLGKEEAWDYDASLAWQWGTAQDSRHRAWAAHLEAGRTFDAP
                     WKPRLALWLNYASGDRTPGDGSNERFDPLFGASYAMYGFSGWFVWRNMLNTAVRLTFQ
                     PAARLRCELLHRAYWLASDTDAWTRGLRRDRTGGSGGFIGQAAEARLVWLVCGNLDFD
                     LACSHFLPGGFTSGTGDAPAAFFFQAAATLKF"
     gene            complement(4922..6085)
                     /locus_tag="PLP58_14770"
     CDS             complement(4922..6085)
                     /locus_tag="PLP58_14770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009959272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HRJ09838.1"
                     /translation="MPSPARYIMIGGFLGAGKTTTVGRLARHLSDQGLKVGLITNDQA
                     GGLVDTKLLRGQGFATEEIAGGCFCCRFNTLVDAAARLNDATKPDVFIAEPVGSCTDL
                     VATVTYPLRRMYGDAFTIAPLAVLVDPVRARRVFGLEEGGTFSSKVAYIFKKQLEEAD
                     LIVISKSDLLDDAQREELRAVLQKEFPLAQVVAASPRQESGLDELFATLMSGEQARRN
                     PMAVDYEVYAEGEALLGWLNATVTLSCDDEFEANDFLKKLAAHVQAKLQRQGVEIAHF
                     KMTFSPDDGIAGELASVNLVRSDYIPELGMELDEPSTGGQLIVNLRAEAEPAALVEAV
                     KAGLEAVSAGFFGLRATLDHEEHFCPGKPVPTYRDGELVMETKGGCQPGSGCC"
     gene            6159..6497
                     /locus_tag="PLP58_14775"
     CDS             6159..6497
                     /locus_tag="PLP58_14775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009964267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HRJ09839.1"
                     /translation="MKCIKMPARKTPPKFDCLSAMKALGEPTRLRLVRQLISGPKPVT
                     ELCESLGATAYNVSKHLRVLKEAGLLEMQKQGQQRIYDLASAFREKLSRNSMTLDLGC
                     CQFHFDKLPE"
     gene            complement(6583..6999)
                     /locus_tag="PLP58_14780"
     CDS             complement(6583..6999)
                     /locus_tag="PLP58_14780"
                     /inference="COORDINATES: protein motif:HMM:NF012314.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HRJ09840.1"
                     /translation="MKRILALAAALSLPLLAADFPKGSPAFLTDFKTATETAKKEGKP
                     LVVVFSAVWCPPCQAMKKTVYPSKEVAALHDKFIWAYLDTDVEANAAAAQKYGVNGIP
                     HIQFLGADGKSLGNQVGGTSASAFAGTLEQVLAKAK"
     gene            complement(7064..8845)
                     /locus_tag="PLP58_14785"
     CDS             complement(7064..8845)
                     /locus_tag="PLP58_14785"
                     /inference="COORDINATES: protein motif:HMM:NF012869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRJ09841.1"
                     /translation="MPETPPSKKLWDRSAWRDAGFFQRYLRPHYNVFIPALIALAFTG
                     GLTVVFIKELAEIAGRGLGGASGPEWMAELDGRLWFLVALVGTQAFVAFWRILLFAKA
                     SERALASLRRETFGKIIRLPMAALNIRRAGELASRLANDVEAMRETLVVTIPMLIRHT
                     VMFSICLALILSMSVKLSLFMIGTIPVVIVLIAVFGARIRRLTRAAQDNLAATQVVVD
                     ESLQSIISVKAFRNEAHELARYDKNLGEYLRTVIRAAAPRASFIAFIIFAFSVALILV
                     TWFAMRMLESGEIAREELTQFAALSGMIAASFMQFPELVAQFQRALGATERVREILAG
                     ETEPDDTDDAPKERFKGRIEMRGVSFAYPSRPDVTVLRDFELHADAGQRIALVGPSGS
                     GKSTGVSLLFRFYDPTGGAVLIDGRDIRGLPLTTLRRNLALVPQEVLLFGGSIRENIA
                     YGRPEAAEDEITAAARKANAHDFITALPDGYDTVVGDRGTQLSGGQRQRIAIARAILA
                     DPAILILDEATSSLDAESERLVQDALDKLMENRTSIIIAHRLSTVRRCHQILVMSGGT
                     VIERGTHEELVGKTGGLYNSLAKLQLE"
     gene            9127..10800
                     /gene="rpsA"
                     /locus_tag="PLP58_14790"
     CDS             9127..10800
                     /gene="rpsA"
                     /locus_tag="PLP58_14790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018968917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HRJ09842.1"
                     /translation="MSAATLAEMIAGSFRELHEGSIVKGRILEIKPQVVLVDIGYKSE
                     GAIPANEFEDEDIQVGDEIEVLLEKLENDEGMVVLSKEKAAYKQNWEKIASVFRDGGL
                     VKGKVKSVVKGGLMVNVGVEAFLPGSQIDIIPPKDLNEYVGKVFEFKIVKINDDRKNI
                     VLSRREVIEAERAEQRQKFLDSVAPGDKVEGVVKNITDFGVFVDLNGMDGLLHITDMS
                     WGRLNHPTELVGIGQRLNLVILEVNRDKERVSLGLKQLQNNPWENIETRYPIGQRVKG
                     KVTKLVAYGAFVEVEEGVEGLVHVSELSWVKRIARPSDVLQVGQELEAVVLGINKDER
                     KISLGVRQLDTNPWDDIDERYPVGTKFTRPIRNLTAYGAFVELEEGIDGMIHVSDLSW
                     TRKVNHPSEMLKKGDEVETVVLGIDKANQRISLGMKQIETDPWSEIDNRFKVGDIVKG
                     KVAKIASFGAFVELEGDIDGLIHISQLSEDHVAKVKDVINVGDPVEARVIKVDKVERR
                     VGLSIKAMAYSDAEIQKESAAFEALRPSTDLVGLEQAFKFATEEWRPGQ"
     gene            complement(10889..12298)
                     /locus_tag="PLP58_14795"
     CDS             complement(10889..12298)
                     /locus_tag="PLP58_14795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009958163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1501 domain-containing protein"
                     /protein_id="HRJ09843.1"
                     /translation="MNRFPWQPLQSRRAMLRQASAGFGYLALRGVLGAEAASNPLAAR
                     KPHFPARAKRVIFLFMKGGPSHVDTFDPKPLLDRDHGKPLPFALPRVTFAKTTTLFKS
                     PWKFKRHGQSGLPVSDLFPHVARCADDLCVIRSMHGTNPAHGGALLKLHTGSDTFVRP
                     SMGAWVSYGLGTENSDLPAFVTICPTLAHGGVNNWGAAFLPAWAQGTPLGNASVPSDQ
                     AVVRHIRGPGSGTTVQRLQLELAAKMSRGHLSGHADAALEARIDSFELAYRMQTAMPE
                     LQDLSGETEATRKLYGLDSPVTANFGRQCLMARRFAERGVRFIQVTHSDSDVQWDQHG
                     NLIKGHTKNAAEVDKPIAGLLHDLKARGLLEDTLVLWGGEFGRTPVAQGTADGRDHNP
                     EGFTMWMAGGGVKAGHAHGATDDYGYYAVENKVHVHDMHATLLHILGLDHERLTFRHA
                     GRDFRLTDVEGHVVKDILG"
BASE COUNT         2326 a         4039 c         4250 g         2252 t
ORIGIN      
        1 attcccgccg cgccttagct tccgccgcaa ggcggtggag tgtggaggga aggggacccc
       61 acagcccgag cggggtctga aaatcacccg tttgcgcaag tgactgatat tcagtcggaa
      121 aaaggtgctc ctgggctctc caaagcgtgt ttttgaaggc tgggaggcca tttttcatgc
      181 cgtggaggcg cgtctccacg ctttggtgac gcacctccac gccgtccgat ggacgctgag
      241 aagttcggag gcgcacctcc acgccgtccg agagacaccg ggaagtttgg aggttcacct
      301 ccgcgccgtc cgagagacgc tgagaagttt ggaggcgcac ctccacatcg tcggcgggcc
      361 tccccaggtg gggcgcttgg tcctccaagc gccgcgtgcg cacgcgcagt ttccgggtgg
      421 cgcggcacag catcaagcgc acccgcagct ccgtacgcgg cgcgttgagg acaacgcgcc
      481 ctaccccaaa ccccaccgcc ttgcggcggc ggccacggcg tagctgcggg cgcaagcccg
      541 tggcaggtgc gtgcgcggag aaggcatccg ctccctcccc caccgccttg tggcggcggc
      601 tacggcgtag ctgcgggtgc aagcccgtgg ccggggtgga agcgcgcagg cgtcagatca
      661 cttcccggcg gctttgcctc cgcgctcggc gggcagggac tggccttcga gccagacgta
      721 ttccttccac gaaccctcgt agaggcgcac cttcgggtag ccgagcacgt gcttgagggt
      781 gaagtagagc aggctgccct cgcgggaggt gccgcagtag cagatgatgt ccttgtccgg
      841 cgtgatgccc gcgctttgca gctcgcgctt catttgggcc gtggacttga actggtgcgt
      901 gttcgcgccg tccatgagcc gcgcccagtg gaagctgatg gctccgggga tgtggccttt
      961 gcgcagccac acgtcgtcat tgccctggaa ctcgttcagc ggacgggcgt ccacgagcag
     1021 gttgccgggt ttgccggcat gggcgcgcac ggttttcaca tccacgccga tgcttttgtc
     1081 gccccgccag ggcaggctgc cgggcgggtt gccaaagtac tcccgcacgg gtttgagccc
     1141 ggccttcacc cagccggcca ggccgccatc cacgacgcgg atgttttgga tgccgaattt
     1201 ctccagcaca tagacgacca tgctggtgct gaggatttcg tcattcggca aggtggcgcc
     1261 ggtggcatag acgaggtgga ggcggtcttt gtccgctccg gcgcgcttga gcagggcttt
     1321 ggtgagggtg tcgggcaggt attgcacagg cacggcctgg tcggtgccgc gcagggtgtc
     1381 gaagttgatg tggtgcgcct ggggcaggtg gccgcggaag tagtctttgt agccgccgcg
     1441 cgtgtcgagc acgatggggc gctgggcggg gtcgggatgg tcgagcagct ttttggcgtc
     1501 agccggtgag atgaggccga tgtcggcatg gacggcggtg gcgagcaggg cgagtgcgag
     1561 gaggtgtttc atggtgggtg gggatcagaa ggtgaagacg aagttgatct tggcgatgaa
     1621 gctgacgctg ccctggtcgg gaaagttggt ccaggtctcc aggacgatgt gcttgctgag
     1681 gtcgtacttg gcgccgacgg agggcagcca gccgcgctgg tcgcgggttt gcacgaggtc
     1741 ggcgttgagg ttgaagttgc ggtcgagcag tttccaggtg cggtccacgc cgagcaggag
     1801 ggtgtcgccc ccggtctgcc tgccgtaacc ggcgtggccg cgcagcaggc cgaagtcgtg
     1861 ggcgagcacg gcgtagctga atggatcgct ccagcgctca tgatcggtga aggcgatgtt
     1921 ggccacgccg agcgcgagca gaccattttc ccaggggctg aaattgacct tggtctggaa
     1981 cagcagcggc ccggtgagat cgggtgcgat ggggctgtcc atgccccatt cgagatccag
     2041 cgggccaaag tcccagccgg tcttgaagcc gagccagaag gagtgctggc cggggccgtt
     2101 ggcggcgaac tggttggcgc cgccgaagct gctgaaggcc tgcacggcca cggtgcggtg
     2161 tggcacggtg tcggcggtgg ggatgttgtt gaggccggtc ggcgtgccgt gggcggtgga
     2221 ggcggcggcg aggagcgcgg cggttaggat gtgagcacgg ttggttttca tggtgagaga
     2281 aggcggaaga tcagcgccgc gagcagcgct gagacgggca gggtgacgac ccaggcggcg
     2341 agcacgggca gtgcggtgcg ccatttggcc tgccgggtgg tgatgccgat gccgagcagc
     2401 gcgcccacgg agacatgcgt ggtggagact ggcagcgcgt gcaggctggc ggtggtgacg
     2461 agcacggcgg tggcgagatt cgcggcgaag ccctggccgg ggttcatgcc ggtgatcttg
     2521 tgcgccagcg tctccgccac ctggcgcgca ctgatgagcc cgcccagcgc catggcggcg
     2581 gcgatgagca tcatgcccgc gctgccctgc agccagccga tgcccgcgag cagcgcggcc
     2641 atcttcggcg tgtcattcag cccgcgcgcg aagcacaccg cgccggcgct gaggaaatgc
     2701 agcgcgtcca gcgcgcgcgt gcggtggtcc ggcgcgaggc gcagcgtttt cagcacgaga
     2761 taaacaccac cgcccgccgc cacggcgagc acggggctga gcagcagcgg cttcacaaag
     2821 ctctcccaca gcttgccgac atgcacgtcc cccgccgccc agcccgcgcc gagcagggcg
     2881 ccggtgagca tgtgcgtggt ggagacggga aagccgagcc gcgtggccag catgcccgcg
     2941 agccccgccg cggcggccac ggcagcgaga aagacaggct gagcggccat ttcatccggc
     3001 acgaggtcct tgccggaaaa agccttcagc atcgcgcccg tcagcagcgt ggcagccacg
     3061 cagcccgccg ccgtcgtcac cgccgcccag agcacggcgg tccggtagct ggccgtgccg
     3121 ctgccaaaga gcgaggccac gcctttgaaa ttggcgttgg ccccgttcgc gtaagccacg
     3181 atgagcacgg cgctgaacag gaggatgagc acggcgctgc ttttgaatgc atttgcccgt
     3241 tttggcaaac tttaatttgc caatattggc aaacaacttc gtcaggctgg cctcatctcg
     3301 cttttttaca gcgcggcgga tttcatgaaa ggaaagcgtt ccgatgctct tgccagattc
     3361 acagcccctc tcatccttga ggcggacctt cgtccgactg ggaatggccg tggctgctgg
     3421 ctgggcctcc tgcggacagg cggaggattt cagtccggtg aaaacgctcg ccgcggcatc
     3481 ggatcgcatc tcggaaagcc ccgcctatgc tcatgcgctg gcgggcagcc tggagtggct
     3541 ggacttcggc atcgagtcgc gcacgcgtta cgaacagcgc tggaacgact atgccacgcc
     3601 cggactgctc agcgatgacg cgctggtgac acgcaacctg ctctggctcg gcatcacgga
     3661 aaagctcgac ccgctgcgcc tctttgtgga ggcggaggat tcccgccgat ttttctccga
     3721 cagaactgcg gatccaaaca tcgagaccgg tctggagctg atccaggcct tcgcccagct
     3781 ttactgggag gacgcgctgg ccggcctgcc tctgcgcatc aacgccggac gcatggcctt
     3841 tgacttcgtg gaccgccggc tggtggcgcg gaaccggaac cggaacacca tcagcgcctt
     3901 cgacggcctg cgcgtccggc tcggggaaga tgccacgcgc tgggagctgg atgcctttgc
     3961 cctgcatccg gtggagcgca ttttcgacgc gcgtgatcag gcggcggaga acatctggct
     4021 tgcgggcctg gccgcgcact ggcgcggcag ctcgccgcat ttcatcgccg agccttactg
     4081 gctgtggctg aaccaggaca atgacaggac cacacccatg cggcgcgaca tccacacgct
     4141 cggctttcat gccttcggac ggctgggcaa agaggaagcc tgggactacg atgccagcct
     4201 ggcctggcag tggggcacgg cgcaagacag ccgccaccgc gcctgggccg cgcacctgga
     4261 ggccggacgc acctttgacg ccccatggaa gccccggctg gccctgtggc tgaactacgc
     4321 cagcggcgac aggacgcctg gcgatggcag caacgagcgc ttcgatccgc tcttcggcgc
     4381 ctcctatgcc atgtacggct tcagcggctg gttcgtctgg cgcaacatgc tgaacacggc
     4441 tgtacggctg acgtttcagc cagccgcccg tctcaggtgc gagttgctgc accgcgccta
     4501 ctggctggcc tccgacacgg atgcctggac gcgcggactg cgccgcgacc gcacgggcgg
     4561 cagcggcggc ttcatcggcc aggcggcgga ggcgcgcctg gtctggctcg tgtgcgggaa
     4621 cctggacttt gacctggcct gctcgcactt cttgcctggc ggcttcacat ccggtacggg
     4681 cgacgcgccc gccgcgttct tttttcaggc cgccgcgacg ctgaagtttt gaggcgcggc
     4741 atcatgcctc agccgttttc atgcgatgga aaaccacgaa tgagcacgaa ttttggaggc
     4801 atcatgtcag gtgagctgaa gcgccgggag atcacccctg ggtgatgccc ccatccctat
     4861 cttctccgca tggattcgtg tccattggtg tccattcgtg gttcaactgc atcgttacgg
     4921 ctcagcagca gccgctgccg ggctggcagc cgcctttggt ttccatcacc agctcgccat
     4981 cccggtaggt gggcacgggc ttgccagggc agaagtgctc ctcatgatcc agcgtggccc
     5041 gcagcccgaa gaagcccgcg ctgaccgcct ccagccccgc tttcacagcc tcgaccagcg
     5101 ccgcgggctc cgcctcggcg cgcaggttca cgatgagctg gccgccggtg ctgggctcgt
     5161 ccagctccat gcccagctcg gggatgtagt cgctgcgcac gaggttcacg ctcgccagct
     5221 cgcccgcgat gccgtcgtcc gggctgaagg tcatcttgaa atgcgcgatc tccacgccct
     5281 ggcgctgcag cttcgcctgc acatgggcgg cgagcttttt gaggaagtcg ttcgcttcaa
     5341 attcatcgtc gcagctcagc gtcacggtgg cattcagcca gccgagcagc gcctcgccct
     5401 cggcatacac ctcgtagtcc acagccatgg gattccgccg cgcctgctcg ccactcatca
     5461 gtgtggcgaa aagctcgtcg agcccgctct cctgccgcgg cgaggcggca acgacctggg
     5521 ccagcgggaa ctctttctgc aaaaccgcgc gcagttcctc gcgctgcgcg tcatcgagca
     5581 gatcgctctt gctgatgacg atgagatcgg cctcttcgag ctgctttttg aaaatgtagg
     5641 ccaccttgga agaaaacgtg cctccctcct ccagaccaaa gacgcgcctg gcgcgcaccg
     5701 ggtccaccag caccgccagc ggcgcgatgg tgaaggcatc cccatacatg cggcgcagcg
     5761 gatacgtcac ggtcgcgacg agatccgtgc agctccccac cggctcggcg atgaagacat
     5821 ccggcttggt ggcgtcattg agccgcgcgg cggcatccac cagcgtgttg aagcggcagc
     5881 agaagcaccc gcccgcgatc tcctccgtgg caaagccctg gccgcgcagc agcttcgtgt
     5941 ccaccaggcc gcccgcctgg tcattggtga tgaggccgac cttcagcccc tgatcgctca
     6001 ggtgccgggc caggcggccg acggtggtcg tcttaccggc tccgaggaag ccgccgatca
     6061 tgatgtaacg cgcaggtgag ggcatgaggt ttgtttgccg ggaggcgcaa gtacgcaagc
     6121 ctcaaccgcg cttcttgcgc aaaaatgccg cgcgtagtat gaaatgcatc aagatgcccg
     6181 cccgcaagac tccgcccaag ttcgactgcc tttccgccat gaaagccctt ggcgagccga
     6241 cacgtctgcg gctggtgcgc cagctcatca gcgggcccaa gccggtgacg gaattgtgcg
     6301 agagcctggg cgccacagct tacaacgtct ccaagcatct gcgcgtgctc aaagaggcgg
     6361 gattactgga gatgcaaaag cagggccagc agcgcatcta tgatctggcc tccgcgtttc
     6421 gggaaaagct ctcgcgcaac tccatgacgc tcgatctcgg ctgctgccag tttcacttcg
     6481 acaagctgcc ggaataagac gatccgggat cagcggcatg cgcgctcgaa aaaaaagccg
     6541 gcggtttccc gccggctggt gtgagactcg gagcggcctg gatcatttgg ccttggccag
     6601 cacctgctcc agcgtgcccg cgaaggctga agcgctggtt ccgccgacct ggttgccgag
     6661 gcttttgccg tcagcgccaa ggaactggat gtgcgggatg ccgttgacgc cgtatttctg
     6721 agccgcagcc gcgttggcct cgacatccgt gtccaggtag gcccagataa acttgtcatg
     6781 cagggcggcg acttccttgc tgggatagac ggtctttttc attgcctggc acggagggca
     6841 ccagacggcg gaaaaaacca cgaccagggg cttgccctct tttttcgcgg tctcggtggc
     6901 ggttttgaaa tcggtgagga aggcggggct gcctttgggg aaatccgcgg cgaggagtgg
     6961 cagggacagg gcggcggcga gtgcgaggat gcgtttcata agtgtgtgtg gattggggtg
     7021 acgcggagaa gacgcgcggc ggctgaattt tattccattg ggatcattcg agctggagct
     7081 tcgccagcga gttgtaaagt ccgccggtct tgccgacaag ctcttcgtgc gtgccgcgtt
     7141 cgatgacagt gccgccggac atgacgagga tttgatggca gcggcgcaca gtgctgagac
     7201 ggtgggcgat gatgatgctg gtgcggtttt ccatgagctt gtccagcgcg tcctgcacga
     7261 gccgctcgct ctcggcatcg aggctgctgg tggcctcgtc gaggatgaga atggcgggat
     7321 cggccaggat ggcgcgggca atggcgatgc gctggcgctg cccgccggag agctgcgtgc
     7381 cgcggtcgcc gacgacggtg tcgtagccat ccggcagggc ggtgatgaag tcgtgggcgt
     7441 tggctttgcg cgcggcggcg gtgatttcgt cctccgcagc ctccggtctg ccataggcga
     7501 tgttttcgcg gatgctgccg ccgaagagca gcacctcctg cggcacgagc gcgagattcc
     7561 ggcgcagcgt ggtcagcggc aggccgcgaa tgtcgcgtcc gtcaatgagg accgccccgc
     7621 cggtcggatc ataaaaacgg aacaagagcg agacgcccgt ggacttgccc gatccgctgg
     7681 ggccgacaag cgcgatgcgc tgcccggcat ccgcatgaag ctcaaagtcg cgcagcacgg
     7741 tgacatcggg ccgggaggga taagcaaagc tgacaccgcg catctcgatc cgtcccttga
     7801 aacgttcctt cggagcatcg tccgtgtcat ccggctcggt ctccccggcc aggatttcac
     7861 gcacgcgctc ggtggctccg agtgcgcgct gaaactgcgc caccagttcg ggaaactgca
     7921 tgaaggaagc ggcgatcatg ccgctgaggg cggcaaactg cgtgagctcc tcgcgggcga
     7981 tctcgccgct ctccagcatg cgcatggcaa accaggtgac gagaatgagc gccacactga
     8041 aggcgaagat gatgaaggca atgaaggagg cgcgcggggc ggcggcgcgg atgaccgtgc
     8101 gcaggtattc acccaggttc ttgtcatagc gcgccagctc gtgggcctca ttgcggaagg
     8161 ctttgacgct gatgatgctc tgcaaactct cgtccaccac gacctgggtg gccgccaggt
     8221 tgtcctgagc cgcgcgtgtc agccggcgga tgcgcgcgcc aaaaacggcg atgagcacga
     8281 tgaccaccgg gatggtgccg atcatgaaca gggagagctt cacgctcatg ctcaaaatga
     8341 gcgccaggca gatggagaac atcaccgtgt gccggatcag cattgggatc gtcaccacca
     8401 gcgtctcgcg catggcctca acgtcattgg ccagacgcga ggccagctcg cccgcgcggc
     8461 ggatgttcaa ggcggccatc ggcagacgga tgattttgcc gaaagtctcc cgccttagag
     8521 aggccagcgc gcgctcggat gccttggcga agagcaggat gcgccagaag gcgacaaagg
     8581 cctgcgtgcc gacaagggcg actagaaacc acagcctgcc atccagctcc gccatccact
     8641 ccggcccgct cgcgccgccg agcccccggc cggcgatttc ggccagttcc ttgatgaaaa
     8701 ccaccgtcag gccgcccgtg aaagcaagcg cgatgagggc gggaatgaag acattgtaat
     8761 gcgggcgcag atatcgctgg aagaagccgg cgtcacgcca ggccgagcga tcccagagtt
     8821 tcttcgaagg tggtgtttca ggcatcagga gcgctgaacg tggcggctgt cacacaaatg
     8881 gcaaggcgcg agagactgtg acgctcaact gccgggattc ccgaccggga ccggcggcat
     8941 gaccgccccg ccgggctgcc cgtttttccc ggcatctttc cccttgccaa tcagtggtct
     9001 cgtccgagaa tggcggccct tttgtccggc ggccctctgg ccgcacggga catcagcccg
     9061 cgccggccct caggagtgga aggtcatgcg cggttccccc accagcactc ccaaccacac
     9121 acaacaatga gcgcagctac actcgctgaa atgatcgcag gatcgttccg cgaacttcac
     9181 gaaggatcca tcgtcaaagg ccggatcctt gaaatcaaac cccaagtcgt cctcgtggac
     9241 atcggctaca agtccgaagg agccatcccc gccaacgagt tcgaagacga agacatccag
     9301 gtcggtgacg aaatcgaagt tctcctggag aaactggaaa atgacgaagg catggtcgtc
     9361 ctctcgaagg aaaaagccgc ctacaagcag aactgggaaa agatcgcctc cgtcttccgc
     9421 gacggcggcc tggtcaaagg caaggtcaag tccgtcgtca aaggcggcct catggtcaac
     9481 gtcggcgtcg aagccttcct gcccggcagc cagattgaca tcatcccgcc gaaggacctc
     9541 aacgaatacg tcggcaaggt tttcgaattc aagatcgtca agattaacga cgaccgcaaa
     9601 aacatcgtcc tctcccgccg cgaggtcatc gaggccgagc gcgccgagca gcgccagaag
     9661 ttcctcgact ccgtggcccc cggcgacaag gtcgaaggcg tggtcaaaaa catcaccgac
     9721 ttcggtgtgt tcgtggacct caacggcatg gacggcctgc ttcacatcac cgacatgtcc
     9781 tggggccgcc tcaaccatcc caccgaactg gtgggcatcg gccagcgcct caacctcgtc
     9841 atcctcgagg tcaaccgcga caaggagcgc gtctcactcg gcctcaagca gctgcagaac
     9901 aacccctggg agaacatcga gactcgttac cccatcggtc agcgcgtcaa gggcaaggtc
     9961 accaagctcg tcgcttacgg tgccttcgtc gaagtggagg aaggtgtgga aggcctcgtt
    10021 cacgtctccg aactctcctg ggtcaagcgt atcgcccgtc cttccgatgt cctccaggtc
    10081 ggccaggaac tcgaagccgt cgtccttggc atcaacaaag acgagcgcaa gatcagcctc
    10141 ggcgtgcgcc agctcgacac caacccgtgg gatgacattg acgagcgcta cccggtgggc
    10201 accaagttca cccgcccgat ccgcaacctc accgcttacg gcgcctttgt ggagctggag
    10261 gaaggcattg acggcatgat ccacgtctcc gacctgagct ggacacgcaa agtcaatcat
    10321 ccgtctgaaa tgctcaagaa aggtgacgag gttgagactg tcgtgctcgg cattgacaag
    10381 gccaaccagc gcatcagcct cggcatgaag cagatcgaaa ccgatccctg gagcgaaatt
    10441 gacaatcgct tcaaggttgg cgacatcgtc aagggcaagg tggccaaaat cgccagcttt
    10501 ggcgccttcg tcgagcttga gggcgacatt gacggactca tccacatctc ccaactcagc
    10561 gaggatcatg tggccaaggt caaggatgtc atcaatgtcg gcgaccctgt cgaagcccgc
    10621 gtcatcaaag tggacaaggt ggagcgccgc gtcggcctct ccatcaaggc catggcctac
    10681 agtgacgccg agattcagaa ggaaagcgcc gccttcgaag ccctccgccc cagcaccgac
    10741 ctcgtcggcc tggagcaggc attcaagttc gccaccgagg aatggcgccc cggtcaataa
    10801 accggacctg ccgatccggt caaaatcacg gagggacgcc gcgaggcgtc cctctttttt
    10861 tatccgcaga ggcgccgtct accgagaatc agcccagaat gtctttcacc acatggcctt
    10921 ccacatcagt gaggcggaag tcgcgcccgg catggcgaaa ggtcaggcgt tcatggtcca
    10981 gacccaggat gtgcagaagg gtggcatgca tgtcatggac atgaaccttg ttttccaccg
    11041 catagtagcc gtaatcgtcc gtggcgccgt gggcatgacc ggccttcact ccgcctcccg
    11101 ccatccacat ggtgaagccc tcgggattgt ggtcgcgccc gtccgccgtg ccctgagcca
    11161 ccggcgtgcg gccaaactcg ccgccccata gaacgagcgt gtcctccagc agtccgcgcg
    11221 ctttaaggtc atgaagcaga cctgcgatgg gcttgtccac ttcggcggcg tttttggtgt
    11281 ggcccttgat gagattgcca tgctggtccc actgcacatc cgagtcgctg tgggtgacct
    11341 gaatgaaacg cactccgcgc tcggcgaagc ggcgcgccat caaacactgg cggccaaagt
    11401 ttgccgtcac cgggctgtca aggccgtaaa gcttccgcgt ggcctcagtc tctccgctca
    11461 ggtcctgcag ctccggcatc gcggtctgca tccggtaggc aagctcgaag gagtcaatgc
    11521 gcgcctctag cgcggcatcg gcatggcctg acaaatgacc ccggctcatc tttgccgcca
    11581 gttccagttg caggcgctgt acggtcgtgc ccgaaccagg cccgcgaatg tggcgcacca
    11641 cggcctgatc agagggcacg ctggcattgc caaggggggt gccctgcgcc caggctggca
    11701 gaaaagccgc gccccagttg ttcaccccgc cgtgggccag cgtggggcag atggtcacaa
    11761 aagcgggtag atcggaattc tcggtgccca ggccgtaact cacccaggct cccatgcttg
    11821 gtcgcacaaa ggtgtcgctt cccgtgtgca acttcagcag cgcaccgccg tgcgccggat
    11881 tggtcccgtg catggagcgg atgacgcaca aatcgtcggc acagcgcgcc acatgcggaa
    11941 agagatcact caccggcagg ccgctctgac cgtgccgctt gaacttccag ggcgatttga
    12001 aaagcgtcgt cgtctttgca aaggtcaccc tcggcagcgc gaacggcagc ggcttgccgt
    12061 ggtcgcggtc caggagcggc ttcggatcaa aggtgtccac atgcgaagga ccgcccttca
    12121 tgaacagaaa gatcacccgc ttggcacgcg ccgggaaatg cggtttccgc gccgccagcg
    12181 gattcgacgc cgcctccgca cccagcacgc cgcgcagcgc cagataacca aaacccgccg
    12241 atgcctgccg aagcatggca cggcgcgact gcaaaggctg ccaggggaaa cggttcacgc
    12301 cggaggatac gccgggaggc tggagacctt tcgacgatgg gaagcccgaa cctcgcgcct
    12361 caacctcccc atgcccgcgc aaccgcattt tgcaccggca tctgccggta aaacttgcct
    12421 cctgctgcgg cattttaaaa agcgcgggcc cgctgcgttg cgggacgcgt ccccgtcgcg
    12481 cctgagggct tcactcctgc gaggaagcag tgccatgagc atcaattcgg cggacctgtg
    12541 gctggcaaag ccggggttgg cttgggcgtc gcagtggcgg gtgcggacgg gatgccgggg
    12601 gcaggcggct gagtagcgag gctctggaca ggcgcacgca gcgggacggg gcggctgttc
    12661 ccctgtgttg gcggagcagg gcggggagcg ggtgtgccgc tcgtcgcctg ctggaccatg
    12721 ggactcatct ctgttttcga cgagctggca ttccctggtg aggctggcag cacacgcaca
    12781 gcggggatgg gggcaggagc cgtgacgcgg ggtttgggcg cggcgggggc aggcgtggct
    12841 gcgcgtgctg cgggcgcgct tgaggct
//
LOCUS       DAOMYJ010000150        12842 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Prosthecobacter sp. isolate AMR_MDS_5629
            NODE_1634_length_12842_cov_21.173536, whole genome shotgun
            sequence.
ACCESSION   DAOMYJ010000150 DAOMYJ010000000
VERSION     DAOMYJ010000150.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867850
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prosthecobacter sp. (wastewater metagenome)
  ORGANISM  Prosthecobacter sp.
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Verrucomicrobiaceae; Prosthecobacter.
REFERENCE   1  (bases 1 to 12842)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12842)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.30
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:34:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,742
            CDSs (total)                      :: 4,684
            Genes (coding)                    :: 4,676
            CDSs (with protein)               :: 4,676
            Genes (RNA)                       :: 58
            tRNAs                             :: 55
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12842
                     /organism="Prosthecobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1634_length_12842_cov_21.173536"
                     /isolate="AMR_MDS_5629"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1965333"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..918)
                     /locus_tag="PLP58_14800"
     CDS             complement(<1..918)
                     /locus_tag="PLP58_14800"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HRJ09844.1"
                     /translation="MSTSSQGSKPSSGWQPPSLEEMRAMLPQYEFESLLGRGGMGAVY
                     KAVQVSLDRPVAIKVLPGDLLGDEDVQFAERFKNEARTMARMSHPGIVNVYDFGQTRA
                     GLLYFVMEFIDGTDVAQMISTQGRLPQEYALAVTTHVCDALNYAHDHGIIHRDIKPAN
                     ILISRDGSVKVADFGLAKQSDPGQSGLTKTNMAMGTPDFVAPEALIAGIPLDGRADLY
                     AVGVMLYQMLTGEIPRGMWVMPGKKLGTDPRFDAIIAKAMQTDRESRYQSAAELRRDL
                     DTLLTTPRASAPAAAASPAPQAAARPASRP"
     gene            complement(1063..1722)
                     /locus_tag="PLP58_14805"
     CDS             complement(1063..1722)
                     /locus_tag="PLP58_14805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ09845.1"
                     /translation="MKENGGHVYGITFDERLLAIDGLGAVGPPVFEVSLAGRGTAGVT
                     RLDHARNQADGHAWLLMSKPAGILLYDVEADTMTTLSTTGNEAVFDPDDPTWIAFVEV
                     PAGESDSEVFIAKIGEDGLEQTRRLTFNSHDDKLPVWITVPQGLPEIKLSRQSPSSLR
                     AAWPLWAAHGFLQTCDDLDSWEDEPFSTSNAVYEIQKSFHLPSLPDRLFFRLSAESSE
                     N"
     gene            complement(1903..2847)
                     /gene="hemC"
                     /locus_tag="PLP58_14810"
     CDS             complement(1903..2847)
                     /gene="hemC"
                     /locus_tag="PLP58_14810"
                     /EC_number="2.5.1.61"
                     /inference="COORDINATES: protein motif:HMM:TIGR00212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004418 - hydroxymethylbilane synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylbilane synthase"
                     /protein_id="HRJ09846.1"
                     /translation="MSKTHLILGTRGSELALAQTRTVTAGLQAGHPGLDIRREIIQTS
                     GDKRQDLRFSEFNQAAGVDKGIFIKELENALQSGAVDAAVHSLKDVPSDLAPGFVIAA
                     VLPRAPVEDVLVTAAPHTLESLPPGARVGTSSVRRAAQLRWLRPDLEIVEIRGNVPTR
                     VKKVTGPDALDAVLLAAAGLLRLGLMQGDRIGIEGMTLHALILDEARFLPAAGQGAIA
                     IECRQDDEESIRLVRALNHEETEARVTAEREFLRLLGAGCQTPVGARTWITGGTLHMA
                     VRVFNEADLSAPPVEKQASHPVREPRALAAGLARGLEK"
     gene            complement(2896..3726)
                     /locus_tag="PLP58_14815"
     CDS             complement(2896..3726)
                     /locus_tag="PLP58_14815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ09847.1"
                     /translation="MKPLLAILLAGCAATAAAQDGWLWIDDGLARLGVDLKAGACIGW
                     FSRSGGENLLNTFDAGRYLQQSYYGDEDGSDWNGKPWRYNPVQGGSWRNEPSAVIEQR
                     AEAGLIYAKTRPRHWATGKVLSEVVMEQWLRLENGAARMKFRVTHNGGTSHAPRHQEL
                     PALFVQPVLDTLVFAGAAGALVKKQPGFPNEYFRPGKGWAAWVDARDQGIGIWCPHAE
                     QLTCYRVRAGDRGDCSYLAPLRTFALTPGMVFEYETALVIGALEEMRARLEKMKPVRW
                     "
     gene            complement(3748..4980)
                     /locus_tag="PLP58_14820"
     CDS             complement(3748..4980)
                     /locus_tag="PLP58_14820"
                     /EC_number="2.6.1.83"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009961032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LL-diaminopimelate aminotransferase"
                     /protein_id="HRJ09848.1"
                     /translation="MAYINENYNKLKAGYLFPEIARRVKAFTEANPEAAKRLIRCGIG
                     DVTEALPQAVRDAMHKAVDEMGDRNSFRGYGPEQGYDFLRNAIADNDFKARGINVKAD
                     EIFISDGSKCDTGNILDIFGAGNKIAITDPVYPVYVDTNVMAGNTGEADASGAYAGLH
                     YLKCTPENGFVPAIPDEAVDLVYLCYPNNPTGATATRAQLEAWVKYARANGTIILYDA
                     AYEAFIQDPEVPRSIFEIEGARDCAIEFRSFSKNGGFTGVRCAVVVIPKGLMGKKKDG
                     TQQAIHPLWSRRHSTKFNGCSYIVQRGAEAIYSPEGKAQVAALIEHYMGNAKLLVEAC
                     RKAGLTVYGGVNAPYVWVGCPDGLTSWQMFDKMLNEANVVITPGSGFGSAGEGCFRIS
                     AFNSRANVEEVCKRIAAL"
     gene            complement(5065..6630)
                     /gene="metG"
                     /locus_tag="PLP58_14825"
     CDS             complement(5065..6630)
                     /gene="metG"
                     /locus_tag="PLP58_14825"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009964315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HRJ09849.1"
                     /translation="MKPYFITTAIDYTNGRPHIGHAYEKVLADVMARFQRLNGREVYF
                     LTGVDQHGQKVQKSAEKEGLTPQQFVEGVTGHFTALWEKLGVRYDGWAATVDERHKRV
                     VCAILQKLHDAGQLYKKSHTGFYSVRQEQFLTDKERGPDGSFGEEWGEVVELREENWY
                     FKLSEHVEWLKGFIRSHPDFIFPANRAKDVLNALDGEPQDLCISRPVERLSWGIPLPF
                     DEKFVNYVWFDALTNYISFAGYLAEECGNEGLPDFSKLWPAEAQVIGKDILFPPHALY
                     WTTMLRALGFTDGQMPRLIVHGWWNIKGAKMSKSLGNVIDPDHLAGIFTPDGLRYYLM
                     RDIATGQDADFSEERILMSYNKELAGGLGNLLNRSLNMAHKYRAGKLAPGAYDDAENQ
                     ALRQTVAAALPAYSSAMDGWAIHDGIAAAWKIVTHANAYVDSTKPFSLAKDPAQAARL
                     DSVLHHLAEALTHASVLLDPVMPVAMQAARAQIGWCLPVGFVLADLKWGLLGDAHQLG
                     TPVPLFPRLETAA"
     gene            6806..8632
                     /locus_tag="PLP58_14830"
     CDS             6806..8632
                     /locus_tag="PLP58_14830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ09850.1"
                     /translation="MPAPPTIDPDAPDTRKPFLPLRPLVALWHLLLPPTQAHADRQTK
                     KARWIAGGVVAALALGVAGLGFAYARPLKDAYKNWRTERLLAEARRLMDDKKYFNAIT
                     KVQQAVSLSPSNVNAVRLNSVFLTAMKRPEAIYFLDKLDQLGATTLDDRKTRVQAYMN
                     LHRGRDASALMEELLAEHAPDEDLMRLAGLVWGESRNKEQLLKPLKTYAENHPEDSAH
                     SLRLARVQTESGDAGETVEGIRRAWRVAEEDGETGLAAIEFLASFESLPPDETARLIE
                     RLRRHPMAGARHQAEALKRQTRLDPARRPQHVQEVLSQARGKKREELVPHVRWLVDQK
                     EFAQVLALVSEEEAKSYQPLLENYLTALTMLGRFDDLERLVADPGVNKLLNETMKAFY
                     RAHLAFVTRKPPREFREALVLARAAAEVEKRGEICLRLAEYAEARGHPDIAQDAYRSA
                     SQMARTERAGHLGLLRASAANGNTALFLESARAAVERWPDDPLFVEQLAYAHLIVGHQ
                     VENSIAELTRLLEQRPDDPGRLLMLALAHWRLRDAGTSIEVLGKADVAGLTPGQRAVY
                     AALAKGQADEEWGRRRAEAIRGIDRQAAMLPEERQFLERALR"
     gene            complement(8657..10090)
                     /locus_tag="PLP58_14835"
     CDS             complement(8657..10090)
                     /locus_tag="PLP58_14835"
                     /inference="COORDINATES: protein motif:HMM:NF025885.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HRJ09851.1"
                     /translation="MAAAPETRILIVDTDPDFLSWAAGHLKAPNVSITAVERAEEALA
                     HYQKTPHDLVLAEVRLPGTSGIELLKRLRQTDPNAMVILFTGIASTSHVIEAMRLGAY
                     DVLGKEKLTYELRTAVETALSSSEARRVTRSQAADAPVDSLQETIIGRSSAMQEVFKM
                     IGRVSRSDAPVMITGESGSGKELVARAIHKFSQRAQKDFVGINITSIPDNLMESELFG
                     HEKGSFTGAVNQRVGRFEQCDGGTLFLDEIGDMPLAVQSKLLRVLQEGEFCRVGSNQV
                     LKCDVRILAATHKDLETAVSKGAFREDLFYRLNVVRIHIPPLRERREDVRLLAEFFLR
                     RQSARRRGVVMRFTEDALALLEAYDWPGNVRELENTIQRACALCNSDVLLPSDIPLGS
                     TGARFANPATTITRMRDALSGLVHIAESSPDIELLPWVTRELARIAHRHFEEDAARAA
                     KFLGIPAADIAAHLEDGQAKKAVKKGK"
     gene            10265..11620
                     /gene="rsmA"
                     /locus_tag="PLP58_14840"
     CDS             10265..11620
                     /gene="rsmA"
                     /locus_tag="PLP58_14840"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: protein motif:HMM:TIGR00755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000179 - rRNA
                     (adenine-N6,N6-)-dimethyltransferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HRJ09852.1"
                     /translation="MNPQKPSKNPKRIISGNDVSPRKSMGQNFLVDPQLARWIADQIE
                     PDDTAFLVEPGPGLGAMTGHLEGRARHLLLIEKDDSLAPRLAGRFAGRADVTVLHADA
                     ARVDMRPWYRHGDVRVIGNLPYSVGGEILKMMLTPPTPVTRAVFMLQKEVCQRIAARA
                     GQDGCGAVSVLVQRDWDVEFLRVVPPEVFQPRPKVDSAVIRLTPRDPATLPVCDRRLF
                     DRLVRLGFGQRRKQMKNLLPEPPEGWQALAGALDKPVTMRAEELSLEEWVRLCRWYEQ
                     RLEADAGQKATEMFDVVNERNEVTGQLTRGEVHARGLLHRAVHVFVINSRGEVFLQKR
                     SHLKDVSPLKWDSSAAGHLDAGESYAACAIREVREENGIEIHDTELAALLPAGAHTDF
                     EFVELRIARHNGPMRCPPEEIACGEWFAPEVISDWVAARPQDFARGFVSCWHEWMRAR
                     A"
     gene            complement(11680..>12842)
                     /locus_tag="PLP58_14845"
     CDS             complement(11680..>12842)
                     /locus_tag="PLP58_14845"
                     /inference="COORDINATES: protein motif:HMM:NF040163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF5722 domain-containing protein"
                     /protein_id="HRJ09853.1"
                     /translation="APHQKGIGVPPLSDARHEIFDLGIRHATVNIVVSSLLRAAPAPG
                     WEPWEFEGRVYYKNERALLGHDTTLRLLAEKQVIASAILLVSNGRAADGAPRSPMVHT
                     EAEPRGIYSIPNLSAETPARLYRAVLHLMAERWSREDGAHGRVTNWILHNEVDQAATW
                     TNMGAQPLARYLETLMRSARLTHHTARLFDPHARVFISLTHHWTRKSGGAGTYIVRDM
                     LEMFAEMARAEGDFEWGVAYHPYPQDLRNPDAWKDDGLTNDFDTPLITPRNIAVLPAF
                     LDQPRFHFQGAPRGILLSEQGFNTPTLSEADQRRQVAGLIYMFRQIRPLKAVEAFHLH
                     RYHDMPEQEGGLRLGIITETGARKLGWEAYKAIGTEREVEFGKLADEVMDAP"
BASE COUNT         2182 a         4028 c         4406 g         2226 t
ORIGIN      
        1 gggacgtgag gcgggccgag cggcggcttg cggagcgggg gaggcggcgg ccgcaggggc
       61 gctggcgcgg ggtgtggtga gcagggtgtc gagatcacgc cgcagttcag ccgcgctctg
      121 gtagcgggac tcgcggtctg tctgcatggc tttggcgatg atggcgtcga agcgcgggtc
      181 ggtgcccagt ttcttgccag gcatgaccca catgccgcgc gggatttccc ccgtgagcat
      241 ctgatagagc atcaccccca ccgcatacag gtccgctcgt ccgtcaagcg ggatgccggc
      301 gatcaaggcc tccggcgcca caaagtccgg cgtgcccatc gccatgttgg tcttcgtcag
      361 tccgctctgg ccggggtcgc tctgctttgc cagaccgaag tcggccacct tcacgctgcc
      421 gtcgcggctg atgaggatgt tcgccggctt gatgtcgcgg tggatgatgc cgtggtcgtg
      481 cgcgtagttc agcgcgtcgc agacatgagt ggtgacggcc agcgcgtact cctgcggcag
      541 cctgccctga gtggagatca tctgcgccac atccgtgccg tctatgaact ccatcacaaa
      601 ataaagcagc cccgcccggg tctggccgaa atcgtacacg ttcacgatgc ccggatggct
      661 catccgcgcc atcgtgcgcg cctcgttttt aaagcgctcg gcgaactgca catcctcgtc
      721 acccagcaga tcaccgggca gcaccttgat ggccaccggc cggtccaggg aaacctgcac
      781 cgccttgtaa accgccccca tgccgccgcg acccagcagc gattcaaact catactgcgg
      841 cagcatggcc cgcatctcct ccagcgacgg cggctgccag ccggaggagg gtttggagcc
      901 ttgggaggag gtgctcatga gttaatccgc aaaataaaca gcaggacgct aaccaggaac
      961 acccaggcaa ggcaagaaag ttttgttttc ctgcaaagag agcggacccg gcacctccct
     1021 tcgtgttcct cgccccgatg ggcatgacgc gcgctcccgc tctcaattct ccgaagactc
     1081 cgcgctcagg cggaaaaaga gcctgtcagg cagagaaggc aggtggaaag acttttggat
     1141 ttcatagacc gcgttcgaag tcgaaaacgg ctcgtcctcc caggagtcga ggtcatcgca
     1201 tgtctgcaaa aaaccgtgcg ccgcccacaa cggccaggcc gccctcaggc tcgacgggga
     1261 ttgccggctc agcttgatct caggcagccc ctgcgggacg gtgatccaca caggcagctt
     1321 gtcgtcatgg ctgttaaagg tcagccgccg ggtttgctcc aatccatcct ctccaatttt
     1381 ggcgatgaag acctcggagt cactttctcc cgcaggcacc tcaacaaacg cgatccaagt
     1441 tggatcatcg ggatcaaaga ccgcctcatt ccccgttgtc gagagcgtcg tcatcgtatc
     1501 cgcctccaca tcatagagca ggatgcccgc aggcttgctc atcagcagcc aggcatgacc
     1561 gtccgcctga ttccgggcat ggtccagccg cgtcacaccc gccgtccccc gcccagccag
     1621 ggacacctcg aagaccggcg gccccacggc acccagacca tcaatcgcca gcagcctctc
     1681 atcgaaggtg atcccgtaaa catgcccgcc gttctccttc acacgcgacc agcagcgcgc
     1741 ccggcaagac gcctgcggat tttgttaaat aaaaaaattc ctgggtgagg agattccaga
     1801 caaattcagg gctcaggagc agggcagcga gtgcgtcact tgacgcaccc ctttgaaaac
     1861 aaagcgttgc taagcagccg gcgcggccgg gtgaaatgga tttcacttct ccagaccgcg
     1921 cgccagcccg gcggcgaggg cgcgcggttc gcggacgggg tggctggctt gcttttccac
     1981 gggaggtgcg gagaggtcgg cttcgttgaa gacgcggacg gccatgtgca gggtgccgcc
     2041 ggtgatccag gtgcgcgcgc cgacgggggt ctggcagccg gcgccgagca ggcgcaggaa
     2101 ctcgcgctcg gcggtgacac gagcctcggt ctcctcatga ttcagagcgc gcacgaggcg
     2161 gatggattcc tcgtcgtcct ggcggcattc gatggcaatg gccccctgcc ctgccgccgg
     2221 gaggaagcgg gcttcatcca ggatgagagc gtgcagggtc atgccctcga tccctatgcg
     2281 gtccccctgc atcaagccga ggcggagcag cccggcggcg gcgagcagga cggcatcgag
     2341 cgcgtcgggg ccggtgactt ttttcacgcg ggtgggcacg ttgccccgaa tttcgacgat
     2401 ctccaggtcc ggcctgagcc agcggagttg ggcggcgcgg cgcacactgc tggtgcccac
     2461 acgcgcgccc ggcggcagac tctcaagtgt atgaggggcg gctgtgacga gcacgtcttc
     2521 gacgggagcg cgcggcagca cggcggcgat gacaaaaccg ggagccagat cagatggcac
     2581 gtctttgaga ctgtgaacgg cggcatccac ggcaccggat tgcagggcgt tttccagctc
     2641 cttgatgaaa atgcccttgt ccactccggc ggcctggttg aactcggaga agcgcaggtc
     2701 ctggcgcttg tcgccgctgg tctggatgat ctcccgccgg atgtcgagac cggggtggcc
     2761 ggcctgcagg cccgcggtga cggtgcgcgt ctgggcgagc gccagttcac tgccgcgtgt
     2821 gcctagaatg aggtgggtct tggacatggg gagggaggtt ttcacgcaaa cggggccgga
     2881 cgcaaaggaa tgctgtcacc agcggacggg tttcattttc tcaaggcgcg cgcgcatttc
     2941 ctcaagcgcg ccgatgacga gagcggtctc gtactcaaag accattccgg gtgtcagcgc
     3001 gaaggtgcgc aacggcgcga ggtaggagca gtcgccccgg tcgccggcgc gcacgcggta
     3061 gcaggtgagc tgctcagcgt gcgggcacca aatgccgatg ccctggtcgc gcgcgtccac
     3121 ccaggcggcc cagcctttgc cggggcggaa gtattcgttg ggaaatccgg gctgcttctt
     3181 gaccagggcg cccgccgcgc cggcgaagac gagggtgtcg agcacaggct ggacaaagag
     3241 ggcgggcagc tcctgatgac gcggggcgtg gctggtgccg ccgttgtggg tgacgcggaa
     3301 tttcatccgc gccgcgccat tctccaggcg cagccattgc tccatgacca cctcggacaa
     3361 taccttccca gtggcccaat ggcggggacg tgttttggca tagatcagtc cggcctcggc
     3421 acgttgttca atgacagcgg acggctcgtt cctccaggag ccgccttgca cgggattgta
     3481 gcgccaaggc ttgccgttcc aatcgctgcc atcctcgtcg ccgtagtagg actgctggag
     3541 gtagcggccc gcatcgaagg tgttgagcag gttttccccg ccggaacgcg agaaccagcc
     3601 gatgcacgcg cccgccttga ggtccacgcc aagacgcgcc aagccatcgt caatccagag
     3661 ccagccatcc tgggcggctg cggtggcggc gcatccggcc aggaggatgg caagaagggg
     3721 cttcatgaga gcggcggcat tcgccggtca cagcgcggcg atgcgcttgc agacttcctc
     3781 gacgttcgcg cggctgttga aggcgctgat gcggaagcag ccttcgccgg cgctgccgaa
     3841 gccgctgccg ggggtgatga ccacgttcgc ttcgttgagc atcttgtcga acatctgcca
     3901 gcttgtaagg ccgtccgggc agccgaccca gacgtaaggc gcgttcactc cgccatagac
     3961 ggtgagtccg gccttccggc aggcttcgac gagcagcttc gcattgccca tgtagtgctc
     4021 gatgagcgcg gcgacctgcg ccttgccttc gggactgtag atggcctcgg caccgcgctg
     4081 cacgatgtag ctgcagccat tgaacttcgt gctgtgacgg cggctccaaa gcgggtggat
     4141 ggcctgctgc gtgccgtctt tctttttgcc catcagtcct ttcggaatga cgaccacggc
     4201 gcagcgcacg ccggtgaaac cgccgttctt cgagaagctg cggaactcga tcgcgcaatc
     4261 gcgggcacct tcgatctcga agatggagcg cggcacttcg ggatcctgga tgaaggcctc
     4321 gtaggcggcg tcataaagga tgatcgtgcc attggcgcgg gcgtatttca cccaggcttc
     4381 aagctgggca cgcgtggcgg tggctccggt gggattgttc gggtagcaga gatagacgag
     4441 gtccaccgcc tcgtcgggga tggcgggcac gaagccgttc tccggcgtgc atttgaggta
     4501 gtgcagcccg gcgtaggcgc cgctcgcgtc cgcctcgccg gtgttgccgg ccatgacgtt
     4561 ggtgtccaca tagacgggat agacggggtc ggtgatggcg atcttgttcc ccgcgccgaa
     4621 gatgtcgagg atgttgccgg tgtcgcactt gctgccgtcg gagatgaaga tctcgtcggc
     4681 cttcacattg atgccgcggg ctttgaaatc gttgtcggca atcgcattgc gcaggaagtc
     4741 gtaaccctgc tccgggccgt agccgcggaa gctgttgcgg tcgcccatct catccacggc
     4801 cttgtgcatc gcgtcgcgca cggcctgcgg cagcgcctcg gtcacatcgc cgatgccgca
     4861 gcggatcagg cgcttcgcgg cctcagggtt cgcctcggtg aaggctttga cgcgccgggc
     4921 gatttcgggg aagaggtaac cggctttgag cttgttgtag ttttcgttga tgtaggccat
     4981 ggcgggaaaa acgggccgcc agtgtggcga gtcggcgcgg ggatgcaata ccgaaggaca
     5041 tcccgaaatg gccaatccgc cgcatcacgc ggcagtctca agcctgggga aaagcggcac
     5101 gggggtgcct agttggtgcg cgtcaccaag caggccccat tttaaatccg ccaggacgaa
     5161 gccgacgggc aggcaccagc cgatctgggc gcgagctgcc tgcatggcca cgggcatcac
     5221 gggatcaagc agcacgctcg catgggtgag ggcctcggcc agatgatgca gaacgctgtc
     5281 gagacgcgcg gcctgggccg ggtctttggc gagcgaaaag ggcttggtgc tgtccacata
     5341 ggcgttcgcg tgcgtgacga ttttccacgc ggcggcgatg ccgtcatgga tggcccagcc
     5401 atccatcgcg gacgaatacg cgggcagcgc cgcggccacg gtctggcgca gcgcctggtt
     5461 ttcggcgtcg tcataggcgc ccggcgcgag cttcccggcg cggtatttgt gcgccatgtt
     5521 caagcttcgg ttgagcaggt tgccgagacc gccggcgagc tccttgttgt agctcatgag
     5581 gatgcgctcc tcgctgaagt cagcgtcctg gccggtggcg atgtcgcgca tcaggtagta
     5641 gcgcaggccg tcgggcgtga agatgccggc gaggtggtcg ggatcaatga cgttgccgag
     5701 gctcttgctc atcttggcgc ctttgatgtt ccaccagcca tgcacgatga ggcgcggcat
     5761 ttgcccgtcg gtgaagccga gcgcgcgcag catggtggtc cagtaaagcg cgtgcggggg
     5821 aaagaggatg tccttgccga tgacctgcgc ctcggcgggc cagagcttcg aaaaatccgg
     5881 cagcccctca ttgccgcact cctccgccag ataaccggcg aagctgatgt agttcgtcag
     5941 cgcgtcgaac cacacatagt tgacgaattt ctcgtcaaag ggcagcggga tgccccagct
     6001 caggcgctcc acggggcgcg agatgcagag gtcctgcggc tcgccgtcga gggcgttgag
     6061 cacgtccttg gcgcggttgg cggggaaaat gaaatccggg tggctgcgaa tgaagccctt
     6121 cagccattcg acgtgctcgc tgagtttgaa gtaccagttt tcctcccgga gttcgacgac
     6181 ctcgccccat tcctcgccga agctgccatc cgggccgcgt tccttgtcgg taaggaactg
     6241 ctcctggcgc acgctgtaga agccggtgtg gctctttttg taaagctggc cggcgtcgtg
     6301 cagcttttgc aggatggcac acaccacgcg cttgtgacgc tcgtccacgg tggcggccca
     6361 gccgtcgtag cgcacgccga gcttttccca aagcgctgtg aagtgtccgg tgacgccctc
     6421 gacgaactgt tgcggtgtca gcccttcttt ttcggcgctc ttctgcacct tttgcccgtg
     6481 ctggtccacg ccggtaagga agtaaacctc gcgcccgttg agccgctgaa agcgcgccat
     6541 cacatccgcc agcaccttct cataagcatg gccgatgtgc gggcggccgt tggtgtagtc
     6601 aatcgcggtg gtgatgaaat aaggcttcac tggtggaaaa atgaaaacca gccataacgc
     6661 gccccgcgcg cgtggcaacc gcggtttcct ccatccggca ggcggaatgc cattgaagcc
     6721 atccgcacga gaagcttgcc gcctggcggg ggcacgcgct atcatggtct cctcccccaa
     6781 ccttttgcct ttttaaaacc cgtccgtgcc agcccctccg acgattgatc cagatgctcc
     6841 cgacacgcgg aagcccttcc tgccgctgcg cccgttggtg gcgctgtggc acctgctgct
     6901 gccgccgacg caggcgcacg cggaccggca gacaaaaaag gcgcgctgga tcgccggcgg
     6961 cgtggtggcg gcgctggcgc tgggtgtggc cggactcggc ttcgcctacg cgcggccttt
     7021 gaaggacgcc tacaagaact ggcggacgga gcgcctgctt gccgaggcgc gccggctgat
     7081 ggatgacaag aagtacttca acgccatcac caaagtgcag caggcggtga gcctctcgcc
     7141 ctcaaatgtc aacgcagtgc gtctgaactc ggtctttctc accgcgatga agcgccctga
     7201 ggcgatctac ttcctcgaca agctcgacca gctcggcgcc acgacactcg acgaccgcaa
     7261 aacgcgcgtg caggcctaca tgaatctgca ccgcggcagg gacgcctcgg cgctgatgga
     7321 ggaactgctg gcggagcacg ccccggacga ggatttgatg aggctggccg gcctggtctg
     7381 gggcgagagc cggaacaagg agcaactgct caagccgctc aagacctacg ctgaaaacca
     7441 cccggaggac agcgcgcaca gcctgcgcct ggcgcgggtg cagacggagt cgggggacgc
     7501 gggggagacc gtcgagggca ttcgcagggc ttggcgggtg gcggaggagg acggcgaaac
     7561 cgggctggcg gccatcgagt tcctggcatc cttcgagtcg ctgccgcctg acgagacagc
     7621 caggctcatc gaaaggctgc gcaggcatcc gatggccggg gcgcggcacc aggctgaggc
     7681 gctgaagagg cagacacggc tggatcccgc acgacgcccc cagcatgtgc aggaggtgct
     7741 gagccaggcg cggggaaaga agcgcgagga actggtgccg catgtgcgct ggctggtgga
     7801 tcaaaaggag ttcgcccagg tgctggcgct ggtctccgag gaggaggcca agagctacca
     7861 gccgctgctg gaaaactacc tcaccgccct gacgatgctg ggccggttcg acgacctgga
     7921 gcgcctcgtc gccgaccccg gcgtgaacaa gctgctcaat gagacgatga aggccttcta
     7981 ccgcgcgcat ctggccttcg tcacgcgcaa gccgccgcgc gagttccgcg aggcgctggt
     8041 gctggcccgg gccgcggccg aggtggagaa gcgcggagaa atctgcctgc gcctggccga
     8101 gtatgccgag gcgcgcgggc atcccgacat cgcccaggac gcctacagga gcgcgtcgca
     8161 gatggcccgc accgagcgcg ccgggcatct gggtctgctg cgcgccagcg cggcgaacgg
     8221 taacaccgcg ctgtttctgg agtccgcgcg ggcggcggtc gaacgctggc cggatgatcc
     8281 gctgtttgtc gagcaactgg cctacgccca cctcatcgtc gggcatcagg tcgagaactc
     8341 catcgccgag ctgacgcgcc tcctggagca gcgcccggat gatcccggac ggctgctgat
     8401 gctggccctg gcgcactggc gtctgcgcga cgcggggacc tcgatcgaag tgctgggcaa
     8461 ggctgacgtg gccggcctca cccccggaca gcgcgctgtt tacgcggcgc tggccaaagg
     8521 ccaggcggat gaggaatggg gacggcgcag ggcggaggcc atccgcggca ttgaccgtca
     8581 ggcagccatg ctgccggagg agcgccagtt cctggagcgg gcgctgcgct gatccggcgc
     8641 cgcaggcagc ggaagatcat ttgccctttt tgacggcctt cttcgcctgg ccgtcttcga
     8701 gatgggcggc gatgtcggcg gcggggatgc cgaggaattt ggcggcgcgc gcggcgtcct
     8761 cctcgaagtg gcggtgggcg atgcgcgcga gctcgcgcgt gacccagggc agcagctcga
     8821 tgtcggggct gctttcggcg atgtggacga gtccgctgag ggcgtccctc atgcgggtga
     8881 tggtggtggc ggggttggcg aaacgcgcgc cggtgctgcc cagcgggatg tcggagggca
     8941 ggagcacgtc ggaattgcac agggcgcagg cgcgctggat ggtgttttcc agctcgcgca
     9001 cgttcccagg ccagtcgtag gcttcgagca atgccagcgc gtcctcggtg aagcgcatga
     9061 ccacgccccg gcgccgcgcg gactggcggc gcaggaagaa ctcggccagc aggcgcacat
     9121 cctcgcgccg ctcgcgcagg ggcgggatgt ggatgcggac gacattgagg cggtaaaaga
     9181 gatcctcgcg gaaggcgccc ttgctgacgg cggtctccaa atccttgtgg gtggcggcaa
     9241 ggatgcgcac atcgcacttg agcacctggt tgctgccgac tcggcagaac tcgccctcct
     9301 ggagcacgcg cagcagcttg ctctgcacag ccaggggcat gtcgccgatc tcgtcgagga
     9361 aaagcgtgcc gccgtcgcac tgctcgaagc ggcccacgcg ctggttgacc gcgccggtga
     9421 agctgccctt ctcatggccg aagagctcgc tctccatgag attgtcaggg atggaggtga
     9481 tgttgatgcc gacgaagtcc ttttgagcgc gctggctgaa cttgtggatg gcgcgtgcga
     9541 cgagctcctt gccgctgccg ctctcgccgg tgatcatgac gggcgcgtcg gagcgcgaga
     9601 cgcggccgat cattttgaag acctcctgca tggccgagga gcggccgatg atggtctcct
     9661 ggaggctgtc cacgggcgcg tcggcggcct gggaacgggt gacgcggcgc gcctcggagg
     9721 agctgagcgc ggtctcgacg gcggtgcgca gctcgtaggt gagcttttcc ttgcccagca
     9781 catcgtaggc gcccagtcgc atggcctcga tgacatggct ggtgctggcg atgccggtga
     9841 agagaatgac catcgcgttc gggtcggtct ggcgcaggcg cttgagcagc tcgatgccgg
     9901 aggtgcctgg caggcgcacc tcggccagga cgagatcgtg cggcgtcttc tgataatgcg
     9961 ccagcgcctc ctcggcgcgc tcgacggcgg tgatgctgac gttgggcgcc ttcaaatgcc
    10021 ccgctgccca ggacaggaag tccgggtcgg tgtccacgat gagaatgcgg gtttcaggag
    10081 cggcggccat tgatggaatt tccacgttcc tagatggtcg tacatgcgcc gtcaatgcca
    10141 gttctggcga ctttgaaaga acacgcggct cggaagcggg gggcggaaag gcctgcggcg
    10201 cccgtgtttt tttggggatt tcaccggcag acaaccattc catgcgtgcc actatgaact
    10261 tcgaatgaac ccgcagaaac cctccaaaaa tccaaaacgg atcatcagcg gcaacgatgt
    10321 cagcccgcgc aaaagcatgg ggcagaattt cctcgtggac cctcagttgg cgcgctggat
    10381 cgccgaccag atcgagccgg atgacaccgc ttttctcgtc gagcccggcc ccggactcgg
    10441 cgccatgacc gggcacctgg agggccgcgc gcggcatctg ctgctcattg aaaaggacga
    10501 ctcgctggcg ccccggctcg ccgggcgctt cgccggacgg gctgatgtga ccgtgctgca
    10561 cgcggacgcc gcgcgcgtgg acatgcggcc ctggtaccgc cacggtgatg tgcgcgtgat
    10621 cggcaacctg ccctactcgg tcgggggcga gattttgaag atgatgctga cgccgcccac
    10681 gccggtgacg cgcgcggtct tcatgctgca aaaagaggtg tgccagcgca tcgccgcgcg
    10741 cgccggtcag gacggctgcg gcgcggtctc ggtgctcgtc cagcgggact gggacgtcga
    10801 gtttctgcgc gtcgtgccgc cggaggtctt ccagccgcgc ccgaaagtgg acagcgccgt
    10861 catccgactc actccgcggg atcccgccac actgccggtg tgcgaccgcc ggctcttcga
    10921 ccggctcgtg cgtctcggct tcggccagcg gcgcaagcag atgaaaaacc tgctgcccga
    10981 gccgcccgaa ggctggcagg ccctggccgg ggcgctggac aaaccggtga cgatgcgcgc
    11041 cgaggagctc tccctggagg agtgggtgcg cctttgccgc tggtatgagc agcggctgga
    11101 ggcggacgcc gggcagaagg ccaccgagat gttcgacgtg gtcaacgagc gcaacgaggt
    11161 taccggccag ctcacccgcg gcgaggtcca cgcgcgcggt ctccttcacc gcgcggtgca
    11221 tgtctttgtc atcaactcgc gcggggaggt cttcctgcaa aagcgctccc atttgaaaga
    11281 tgtctcgccc ttgaaatggg acagcagcgc cgccgggcat ctcgacgccg gggagagcta
    11341 cgccgcctgc gccatccgcg aggtgcgcga ggagaatggc atcgaaatcc acgacaccga
    11401 gctggccgcg ctgctgcccg ccggcgcgca cacggacttc gaattcgtcg agctgcgcat
    11461 cgcccgccac aacggcccga tgcgctgccc gccggaggag atcgcctgcg gcgagtggtt
    11521 cgcacccgaa gtcatcagcg actgggtggc cgcgcgcccg caggacttcg ccaggggctt
    11581 cgtctcatgc tggcatgagt ggatgagggc gcgcgcatga aacatgctga ctgcattccc
    11641 gccggggccg cgaatcaaat ccccccgccc ccccgccgct cacggcgcgt ccatcacctc
    11701 gtcggcgagt ttgccaaact ccacctcccg ctccgtgccg atggccttgt aagcctccca
    11761 gccgagcttg cgcgcgccgg tctcggtgat gatgccgaga cgcaggccgc cctcctgctc
    11821 cggcatgtca tggtagcggt gcagatggaa ggcctcgacg gccttgagcg ggcggatttg
    11881 ccggaacatg taaatgaggc ccgccacctg gcggcgctgg tcggcctcgc tgagcgtggg
    11941 tgtgttgaag ccctgctcgg agagcaggat gccgcgcgga gcgccctgga agtgaaagcg
    12001 cggctggtcg aggaaggcgg gcagcacggc gatgttgcgc ggcgtgatga gaggggtgtc
    12061 gaagtcgttg gtgaggccat cgtccttcca ggcgtctggg ttgcgcaaat cctgcggata
    12121 ggggtgataa gccacgcccc actcgaagtc accctccgcg cgcgccatct ccgcgaacat
    12181 ctccagcatg tcgcgcacga tgtaggtgcc ggcgccgccg ctcttgcgcg tccagtggtg
    12241 ggtcagcgag atgaagacgc gcgcgtgcgg atcaaaaagc cgcgccgtgt ggtgggtgag
    12301 ccgggcggag cgcatgaggg tctccagata gcgggccagg ggctgcgcgc ccatgttggt
    12361 ccaggtggcg gcctggtcca cctcgttgtg caggatccag ttggtcacgc gaccatgggc
    12421 gccgtcctcg cgcgaccagc gctcggccat gagatgcagc acggcgcgat acaagcgcgc
    12481 cggggtctcg gcggagagat tggggatgga gtaaatgccg cgcggctcgg cctcggtatg
    12541 caccatgggg ctgcgcggcg cgccatccgc ggcgcgcccg ttggagacga gcaggatggc
    12601 gctggcgatg acctgctttt ccgccagcag gcgcagggtg gtatcgtgcc ccagaagcgc
    12661 gcgctcattt ttataataga cgcgcccctc gaactcccag ggctcccagc cgggcgcggg
    12721 cgcggcgcgc aggaggctgc tgaccacgat gttgacggtg gcgtggcgga tgccgagatc
    12781 gaagatctca tgccgggcgt cactcagcgg cggcacgccg atgcctttct ggtgcggcgc
    12841 ct
//
LOCUS       DAOMYJ010000151        12792 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Prosthecobacter sp. isolate AMR_MDS_5629
            NODE_1643_length_12792_cov_19.819502, whole genome shotgun
            sequence.
ACCESSION   DAOMYJ010000151 DAOMYJ010000000
VERSION     DAOMYJ010000151.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867850
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prosthecobacter sp. (wastewater metagenome)
  ORGANISM  Prosthecobacter sp.
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Verrucomicrobiaceae; Prosthecobacter.
REFERENCE   1  (bases 1 to 12792)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12792)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.30
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:34:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,742
            CDSs (total)                      :: 4,684
            Genes (coding)                    :: 4,676
            CDSs (with protein)               :: 4,676
            Genes (RNA)                       :: 58
            tRNAs                             :: 55
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12792
                     /organism="Prosthecobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1643_length_12792_cov_19.819502"
                     /isolate="AMR_MDS_5629"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1965333"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(51..1091)
                     /locus_tag="PLP58_14850"
     CDS             complement(51..1091)
                     /locus_tag="PLP58_14850"
                     /inference="COORDINATES: protein motif:HMM:NF020123.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HRJ09854.1"
                     /translation="MTRILTACLLIPLSASAQTLAESIRALIDEYENSVRANTQKILA
                     ATSEEERASFRASVPSAAPCAEKVLRLIEASPDDPGGAAGVAWLVTQAGGLPQGARAL
                     EMLGTTHVRSAGIAAAVKALEHQPSALALPLLKTLRERNPHPEEQAAAAYATGVHHFR
                     RYENAASEEERAAAKEEAMAAFQEVAARHAGVSIQGFPLADQAGRMLFELGNLSVGDP
                     APAIEGADLDGGAFTLKDHLGKHVVLVFWGGWCHACHGVMPQVNEFSREHQDKAVVLG
                     INTDIPAEARKAMSDFQISFRNWSDGATSGPITSMFNLRGFPTIHLIGPDGRIVLKNT
                     HLAAIREHLARH"
     gene            1359..1487
                     /gene="rpmH"
                     /locus_tag="PLP58_14855"
                     /pseudo
     CDS             1359..1487
                     /gene="rpmH"
                     /locus_tag="PLP58_14855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006404565.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
     gene            1562..1915
                     /gene="rnpA"
                     /locus_tag="PLP58_14860"
     CDS             1562..1915
                     /gene="rnpA"
                     /locus_tag="PLP58_14860"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR00188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004526 - ribonuclease P activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HRJ09855.1"
                     /translation="MRLPSRLRMKESREFARIKKEGRSQAGRFFVLAVLPDESVPDFR
                     FGLVTGKKLGDAVARNRLRRQMREIIRAARPAIRPGRLFVTIARWRAPGADFAELEQD
                     WLRLAKRQGLLAEPS"
     gene            1924..2163
                     /gene="yidD"
                     /locus_tag="PLP58_14865"
     CDS             1924..2163
                     /gene="yidD"
                     /locus_tag="PLP58_14865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002474682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HRJ09856.1"
                     /translation="MRLLIRGYQRFLSPVIHFLGGPGSGCRFTPTCSEYFLQAVESHG
                     ILRGGWLGLRRIARCHPWGGQGHDPVPEKCRPHQH"
     gene            2178..4010
                     /locus_tag="PLP58_14870"
     CDS             2178..4010
                     /locus_tag="PLP58_14870"
                     /inference="COORDINATES: protein motif:HMM:NF026199.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YidC/Oxa1 family insertase periplasmic-domain
                     containing protein"
                     /protein_id="HRJ09857.1"
                     /translation="MDRKAWIVITLCAVGMALNYFFAVQNQQAFAAQQAEAAKNAPPA
                     AEAPAKSASAPPAGEAPATIAAAPNAAALPEEKHSIQKGTVTYHFTTRGGGIEKAVLG
                     GTDQIVINGHGKEPVGALRREAAGLDATPYRIIEKSGTGVTFEGSTPDGITIRKAFSL
                     NGDDKADEHLLGLTLTLTNTGAAPHRSEEYHLFTGTAASMSPDEVLKPSFFWNDAGDA
                     DYKDANWFGGGMFSSEKSEFRASHARLRYSGVMSRFYATIVSREGATDKPGKVWATRF
                     LVDHSNDKYKDMDAGRADWAVQGAMSLPPVEMEPGKSQTEEYQIYMGPKEYHRLKTLG
                     GQREFIMFYGMFGIISKMLSNIMRWMHDLSGNWGVAIILLTLVIRTVLWPLQSKAQYS
                     MKRMALLGPKMKELQEKFKDEPQKQQMEVMKLYKDYGVNPVGGCLPMLLQIPIFFAFY
                     SVLQNAAELRGQPFLWVKDLSIADTIHTLVFPFHLPLMGNDFDINPLPLIMGVTMILQ
                     MKLTPQPATVDKTQKIMFAIMPFFFLFICYNFAAALSLYWSTQNVYAIFQSWIMRMYM
                     KDPVLEKVERPPPKGPPPQNPFFNPGNPGHKDKRNKNKPPKLGG"
     gene            4133..5743
                     /locus_tag="PLP58_14875"
     CDS             4133..5743
                     /locus_tag="PLP58_14875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007416740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HRJ09858.1"
                     /translation="MLTLRDVTKTFNARTLFSKANMTVNYSERVALVGPNGAGKSTLF
                     SLILRQDEPDAGEVVRDEWTTLGYLPQESEPVGQETVLDLATGRAGEIERLEAILREH
                     EARGDVSSAEYNEAHSKHDALNDPQAEAKAKKILRGFGYKEEDFDKPAREYSGGWVMR
                     AHLARLLVIEPDLLLLDEPTNHLDLLSLLWFRNYLKNYPGAILLISHDRDFMDELIES
                     VYEIEDSRLIQYQGNYSEYLKQKEENWERAYQAWKNQQKEIEAMQEFIDRFRSVASKA
                     AQAQSRERQLEKMEKLERPKPLRKAFRFNFPQPQRSGQRVIQLIDIHQAYGEKKVYQG
                     LDLEVEKGERTVLVGPNGSGKSTLIKIMAGVVPFQKGERKFGTNVRMGYFSQHRSDTL
                     DPECTVLEEIKRCAPELREDDARSVLGSFLFKREDVFKKCKVLSGGEKSRLNLVKFLV
                     DPPNLLLMDEPTTHLDIWGIEGLILALQKFEGTLVFISHDVHFIRSLATKVLHINSGV
                     VTPYSGGYDYYLEKTGAEENARAAVVAG"
     gene            complement(5749..6093)
                     /locus_tag="PLP58_14880"
     CDS             complement(5749..6093)
                     /locus_tag="PLP58_14880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ09859.1"
                     /translation="MKAALLSLFTLTCALALSSCYMATLPPGPGFGGQPLGYNRPFHP
                     GHHQPGWQSPGYGVGVWQTGYDYGRSDRMRGLNYYPARYRSRVSEKHRANFYAGYRDG
                     YYNSGGGIWRRR"
     gene            6166..6855
                     /gene="surE"
                     /locus_tag="PLP58_14885"
     CDS             6166..6855
                     /gene="surE"
                     /locus_tag="PLP58_14885"
                     /EC_number="3.1.3.6"
                     /inference="COORDINATES: protein motif:HMM:NF001493.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'/3'-nucleotidase SurE"
                     /protein_id="HRJ09860.1"
                     /translation="MDILITNDDGIDAPGLAALERAACGMGRVVVVAPMREQSMCGHR
                     VTTRSPIRMEQRSQDRWAVDGTPADCVRLALRALDLRPAWVLSGVNAGGNMGQDIVIS
                     GTVAAAREAAYHGLRAMALSHYLIRDLAVDWLRVGEWAGELMRELSGAALADGEFWNV
                     NLPHHPPGTLGLPERLPCHPARSPLNVSYAPTEHGHVYTASYAERPQDPGSDVEVCFS
                     GRVAVSRLRIG"
     gene            6935..7879
                     /locus_tag="PLP58_14890"
     CDS             6935..7879
                     /locus_tag="PLP58_14890"
                     /inference="COORDINATES: protein motif:HMM:NF040322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ09861.1"
                     /translation="MFIAFWNLENLFDIETSTERQDWLKIRLGKELQGWTAAVLEAKL
                     DQLAAGIKAMNSGAGPDVLGVCEVENRPVLEKLARKLNLPGRSYKVAHENSRDGRGID
                     VAVIYDGKLFTVDGKWSHWVMRRTGTRDLLQVNLKTRDAAAETLVVIVNHWPSRSGGE
                     HASEPYRMLAGETLAYWHSRIWEVLGKDALIVAMGDFNDEPFNRSLTDYALADRERQK
                     VLRAASAPRFLNLMLPLLAAEGSYVFDGRKNMLDQFLVSRGLLNATGRFRLRDVAVSI
                     IRPPGVTGAPVRFSRPSETGGVNTAGYSDHFAIGLKLG"
     gene            complement(7849..8289)
                     /locus_tag="PLP58_14895"
     CDS             complement(7849..8289)
                     /locus_tag="PLP58_14895"
                     /inference="COORDINATES: protein motif:HMM:NF037970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HRJ09862.1"
                     /translation="MIPGPPGVMTKLLTTRWLWWAGILLWAGLLWWLSSRSALPSGPE
                     FPFKDKVLHCAYFSGGAFCLATALFAARLESPPSWAWLLAGFIFTALTGALDELHQSF
                     TPGRMGNDPGDWLADVCGGVLGGWLAWRARRMCPDQPSFNPMAK"
     gene            complement(8286..8609)
                     /locus_tag="PLP58_14900"
     CDS             complement(8286..8609)
                     /locus_tag="PLP58_14900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009964674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family antitoxin"
                     /protein_id="HRJ09863.1"
                     /translation="MEPKRRGDTTQSSRPGDELAYVRAWGDVPAFSSEDAEGRFWASH
                     QVDPRLMQMSIHRNDVRESTTITLRFDPRMLSRIKRIARRRYLNYQSMIKQWLSERME
                     QETRE"
     gene            complement(8615..8893)
                     /locus_tag="PLP58_14905"
     CDS             complement(8615..8893)
                     /locus_tag="PLP58_14905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009964673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BrnT family toxin"
                     /protein_id="HRJ09864.1"
                     /translation="MDFDWIGVTFDLTKLTPKDIEESFEDPFSMKLLPDDNSEGAEAR
                     YYNLGKSLGGRGIFSVFWTDGKRYRVIFARDMTPTEADFFERKRAEDV"
     gene            complement(8929..9306)
                     /locus_tag="PLP58_14910"
     CDS             complement(8929..9306)
                     /locus_tag="PLP58_14910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020494516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HRJ09865.1"
                     /translation="MVKKLLHTRYRVNDLEKTAAFYKEVLGLEEIKRHKSPRGSELVF
                     FKTPNSDELIEICSFPASGPVTMCQDLTHLAFEVEDIEAFARHAEAKGYPLSDGPTES
                     SSGRFAFIDAPEGYEIELIEYRQ"
     gene            9462..9848
                     /locus_tag="PLP58_14915"
     CDS             9462..9848
                     /locus_tag="PLP58_14915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009963862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ApaG domain"
                     /protein_id="HRJ09866.1"
                     /translation="MNTRFEPLAGLTVTVDDVHYDPSRPAPPDRPHPFLYHVSIHNGS
                     PETVSIFGRKWIVRDTDGDTLVVEGDGVVGQFPKLEPGETFSYNSYHVIKSESTATGS
                     FFGTTGEGRPVCARIPLFQMQPPLMA"
     gene            complement(9915..10703)
                     /locus_tag="PLP58_14920"
     CDS             complement(9915..10703)
                     /locus_tag="PLP58_14920"
                     /inference="COORDINATES: protein motif:HMM:NF019887.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD hydrolase family protein"
                     /protein_id="HRJ09867.1"
                     /translation="MAARKTSFILSFDFDGTLVHPGSDPVFHPGLGRMIQQLRAQGAA
                     WVINTGRSLSHTLQGLAQYGIFMEPDYIIAMECDIYRPGMLGGWKDFGPWNRQARKAH
                     ERFVKDHQASLDSIQEMVETQTAANFLEGEYGELGIVAASDEEMDRICAFIDSQAARF
                     PDIGYHRNGIYLRFSHSAHSKGTALSELARLLDLDSTRCFAAGDNHNDLSMLHPRHAG
                     MIATPGNALPAVKAHVLGHGGFVAGKNASEGMMEALSHYFGAAK"
     gene            complement(10772..11698)
                     /locus_tag="PLP58_14925"
     CDS             complement(10772..11698)
                     /locus_tag="PLP58_14925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009964427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ09868.1"
                     /translation="MVHTRPPLVMSSVLLLILAGGAGMSRGQSAPKVTSTASVAAFQG
                     RAESGQNSLPQTTGPRVTVPTMPRLGYARYPWKTDITATVFWVGEKPAENNPTPNNKS
                     SWDTEWEKNFGGYDDPDPSKRNANYCPAEFVPGQNPFYIALPYNDCQDHKAHKSEAEK
                     VIPWFKDRFVRSGKSVLKGTWVAIRYGNRVCYAQWEDCGPFVTDDHEYVFGSARPKNT
                     SNSGAGIDISPAVRDYLGIPSKARCDWRFVDTSEVPPGPWRTLGANNPFVRAQEEQKA
                     RQQSESLSRLDELRRLRDAYFQQNGNDAYRTR"
     gene            complement(11832..>12792)
                     /locus_tag="PLP58_14930"
     CDS             complement(11832..>12792)
                     /locus_tag="PLP58_14930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ09869.1"
                     /translation="VVLATGNEAPADLPGAESLQENPAWAGNPWLPWHERLPPDTESI
                     VLLGTGLTTVDAIITLRALGWLGRIHAVSRHGWLPHAHFRGVEYPDFPPADTDLAALG
                     LDRLAALMEDHCARLRAMGANPAIIVDKMRPHTQRIWQGFSLEQRLAFTRQHAARWNV
                     LRHRIAPEIHAQITSAQLTGQLQVHAENIEHVEAAGARVRVHFEGGGQLEGGLVINAT
                     GPRTRFSSTRSVLLRNLLRRGLISSDDMDMGARVDGSHTVIDRDGGVSPFLLALGPLL
                     RGTYWETIAVPELRGQALRVAETILGQKSTPAAETPVMMEYMI"
BASE COUNT         2284 a         3941 c         4194 g         2373 t
ORIGIN      
        1 ggaggcgcgg cgggcgggct gaggggcggg gaaccggtct gtgggcgggg tcagtgccgg
       61 gcgaggtgct cgcggatggc ggcgaggtgg gtgtttttga gcacgatcct gccgtccggg
      121 ccgatgaggt ggatggtggg gaagccgcgc aggttgaaca tggaggtgat ggggccgctg
      181 gtggcgccgt cagaccagtt gcggaagctg atctggaagt cgctcatggc tttgcgcgcc
      241 tcggcgggga tgtcggtgtt gatgccgagg acgacggctt tgtcctggtg ttcgcggctg
      301 aactcgttga cctggggcat gacgccgtgg caggcatggc accagccgcc ccaaaagacg
      361 aggacgacgt gcttgccgag gtggtctttg agcgtgaagg cgccgccgtc gaggtcggcg
      421 ccctcgatgg cgggcgcggg gtcgccgacg gagaggttgc ccaactcgaa gagcatcctg
      481 ccggcctggt cggcaagcgg gaagccctgg atgctgacgc cggcatgtct ggccgcgacc
      541 tcctggaagg ctgccatggc ctcctccttg gcggcggcgc gctcctcctc gctggcggcg
      601 ttttcatagc ggcggaagtg atgcacgccg gtggcatagg cggcggcggc ctgctcctcg
      661 gggtgcgggt tgcgctcgcg aagggtcttt aacaagggca gggcgagggc ggagggctga
      721 tgctccagcg ccttcactgc ggcggcgatg cccgcgctgc gcacatgcgt ggtgccgagc
      781 atctccagcg cgcgcgcgcc ctgcggcaag ccgccggcct gggtgacgag ccaggcgacg
      841 ccggccgcgc cgccggggtc gtccgggctg gcttcgatga gtcggaggac tttctcggcg
      901 cagggcgcgg cgctgggcac ggaggcgcgg aaactggcgc gctcctcctc gctggtggcg
      961 gcgagaattt tctgagtgtt ggcgcgcacg ctattctcat actcgtcaat gagggcgcgg
     1021 atgctttccg ccagggtctg ggcggaggcg gaaaggggga tgagcaggca ggcggtgagg
     1081 attcgggtca tggcggtggt tgcgcgggta aagttatggg tattccaaag tcctgccagg
     1141 gggatgtttc aggttgatga atattacatg acacgagctg gcggcggtcg aacccggtgg
     1201 aaatgccgga aatccagggc gaaaggcgtg aaaaccgaag gcaaacaggt gatttgacaa
     1261 accctggccg ccggttagtc tcaccgcccc tttgccgggg tggcctgttc atattgccgc
     1321 cttggatcgg cgaaaccctc cactctcatc tctgatttat gcccaaaaga acctaccagc
     1381 cctccaagcg cacccgcaaa cgccagtttg gtttccgcgc ccgcatgaag accgcgaacg
     1441 gtcgtgacat catccgccgc cgccgcgccc ggggacgcca gcgcctcatg cccgtgggtg
     1501 tggagattca ttacaagcgc cacgtgcagc agcacgcctg agtctttgag aaggcccgcc
     1561 catgcgcctg ccctcacgcc ttcgcatgaa ggagtcccgc gagttcgcgc ggatcaagaa
     1621 ggaggggaga agccaggcgg gccgtttttt tgtgctggct gtgctgcctg atgagagcgt
     1681 ccctgatttc cgctttggcc tggtcacggg caaaaaactc ggggacgcgg tcgcacgcaa
     1741 ccgtcttcgg cgccagatgc gcgagatcat tcgcgcggcc aggccggcga ttcgcccggg
     1801 gaggctgttt gtcaccatcg cccgctggcg cgcgccgggc gcggacttcg ccgaactgga
     1861 gcaggactgg ctccggctcg ccaaacgcca gggcctgctg gcggagccgt catgaaatgg
     1921 atcgtgcgtc tgctcatccg gggctaccag aggtttctct cccccgtgat tcatttcctg
     1981 ggcggtcccg gctccggctg ccggttcacg ccgacttgct cggaatactt tttgcaggcc
     2041 gtggagagcc acggaatcct gcgcggcggc tggctgggcc tgcgccgcat cgcccgctgc
     2101 catccctggg gcgggcaggg gcatgaccct gtgccggaga aatgccgccc ccatcaacac
     2161 tgaacccttt ttcctcaatg gaccgcaaag cctggatcgt catcaccctc tgcgccgtcg
     2221 gcatggcgct gaactacttc ttcgccgtgc agaaccagca ggccttcgca gcgcagcagg
     2281 ccgaggcggc aaagaacgcg ccacccgccg cagaggcgcc cgcgaagagc gcgtccgcgc
     2341 cgcccgcggg tgaggcgccg gccacgattg ccgcggcgcc aaacgccgcc gctttgcccg
     2401 aggagaagca cagcattcag aaaggcaccg tcacctacca cttcaccacg cgcggcggcg
     2461 gcatcgaaaa ggccgtcctt ggcggcacgg accagatcgt gatcaacggc cacggcaagg
     2521 aacccgtggg cgccctgcgc cgcgaggccg ccggactcga cgccacgccc taccggatca
     2581 tcgagaaaag cgggacgggc gtcaccttcg aaggctccac ccctgacggg atcaccatcc
     2641 gcaaggcgtt ttccctcaat ggtgatgaca aggccgacga gcatcttctc ggactcaccc
     2701 tcacgctcac aaacactggc gcggcgccgc accgctccga ggagtaccac cttttcaccg
     2761 gcaccgccgc ttccatgtcg cccgacgagg tgctcaagcc cagctttttt tggaatgacg
     2821 ccggagacgc cgattacaag gacgccaact ggttcggtgg cggcatgttt tccagcgaga
     2881 agagcgagtt ccgcgccagc catgcgcgcc tgcgctactc gggtgtgatg agccgctttt
     2941 acgccacgat cgtgagccgc gagggcgcga ctgacaagcc cggcaaggtc tgggccacgc
     3001 gcttcctcgt ggaccactcc aacgacaaat acaaggacat ggacgcgggg cgcgcggact
     3061 gggccgtcca gggcgccatg agtctgccgc cggtggagat ggaacccggc aagtcccaga
     3121 ccgaggaata ccaaatctac atggggccga aggaatacca ccggctcaag acgctgggcg
     3181 ggcagcggga gttcatcatg ttctacggca tgttcggcat catcagcaag atgctctcga
     3241 acatcatgcg ctggatgcac gacctgagcg gcaactgggg cgtggcgatc atcctgctca
     3301 ccctcgtcat ccgcaccgtg ctctggccgc tccagtccaa ggcgcagtac tccatgaagc
     3361 gcatggcgct gctcggtcca aagatgaagg aactccagga gaagttcaaa gacgagcccc
     3421 agaagcagca gatggaggtg atgaagctct acaaggacta cggtgtgaac ccggtgggcg
     3481 gctgcctgcc catgctgctg cagatcccca tcttctttgc cttctacagc gtgctgcaaa
     3541 acgccgccga gctgcgcggg cagcccttcc tgtgggtgaa ggacctctcc atcgccgaca
     3601 ccatccacac gctggtcttt cccttccatc tgcccctcat gggcaacgac ttcgacatca
     3661 atccgctgcc gctcatcatg ggggtgacga tgatcctgca gatgaagctg acaccccagc
     3721 cggccacggt ggacaagacg cagaagatca tgttcgccat catgcccttc ttctttctct
     3781 tcatctgcta caacttcgcc gccgccctct ccctctactg gtccacccag aacgtgtacg
     3841 ccattttcca gagctggatc atgcgcatgt acatgaagga tccggttctg gagaaagtgg
     3901 agcgcccgcc gcccaaaggc ccgccgccgc agaacccctt cttcaatccg gggaatcccg
     3961 gtcacaagga caagaggaac aaaaacaagc cgccaaagct cggcggttag cctctctggc
     4021 cgggtgtcat tcttttgacc cgcgccaaaa accgcgaagc gggcggagac ctcgggcgcg
     4081 cggcaaaata ctggctgcat tccacaggcg gcggtgctaa tgcgccggtt ccatgcttac
     4141 ccttcgcgac gtcaccaaga ccttcaacgc ccgcacgctt ttcagcaaag cgaacatgac
     4201 cgtgaactat tccgagcgcg tggccctggt ggggcctaac ggcgcgggca aatccactct
     4261 cttctcactc atcctccgcc aggacgagcc ggacgcgggc gaggtggtgc gcgacgagtg
     4321 gaccacgctg ggttatctgc cgcaggaaag cgagcctgtg ggacaggaga cggtgctcga
     4381 tctggccacc ggacgcgcgg gagagattga aaggctggag gccatcctgc gggagcacga
     4441 ggcgcgcggt gacgtctcct ccgccgaata caacgaggcg cactccaagc acgacgcgct
     4501 caacgaccct caagccgagg ccaaggcgaa gaaaatcctc cgcggcttcg gctacaagga
     4561 ggaggacttc gacaaacccg cgcgcgagta ctccggcggc tgggtgatgc gggcgcatct
     4621 ggcgcggctg ctggtcatcg agccggacct gctgctgctc gacgagccca ccaaccacct
     4681 tgacctgctc tcgctgctct ggttccgcaa ctacctgaaa aactaccccg gcgccatcct
     4741 gctcatctcc catgaccgcg acttcatgga cgagctgatc gagtcggttt atgaaatcga
     4801 ggacagcagg ctcatccaat accagggcaa ctactccgaa tacctcaagc agaaggagga
     4861 gaactgggag cgcgcctatc aggcgtggaa gaaccagcag aaggagatcg aggccatgca
     4921 ggagttcatt gaccgcttcc gctccgtggc ctcaaaagcg gcgcaggcgc agagccgcga
     4981 aaggcagctc gaaaagatgg agaagctgga gcgcccgaag ccgctgcgta aggccttccg
     5041 cttcaatttc cctcagccgc agcgcagcgg ccagcgcgtg atccagctca ttgacatcca
     5101 ccaggcctat ggtgagaaga aagtctatca gggattggac ctcgaagtcg agaagggcga
     5161 gcgcaccgtg ctggtggggc cgaacggttc gggcaagtcc accctcatca agatcatggc
     5221 cggcgtggtg ccctttcaga agggcgagcg gaagttcggc acgaatgtga ggatgggcta
     5281 tttctcccag caccgctccg acacgctcga ccccgaatgc acggttctgg aggagatcaa
     5341 acgctgcgcg ccggagttgc gcgaggatga cgcgcgcagc gtgcttggca gcttcctctt
     5401 caagcgggag gatgtcttca agaaatgcaa ggtgctcagc ggcggagaga aaagccggct
     5461 caacctggtc aagtttctcg tggatccgcc aaaccttctg ctcatggacg agccgacgac
     5521 gcacctggac atctggggca tcgagggtct catcctcgcg ctgcaaaaat tcgagggcac
     5581 gctggtcttc atctcccatg acgtgcattt catccgcagc ctggcgacca aggtgctgca
     5641 catcaactcg ggcgtcgtca ccccctacag cggcggctac gactactacc tggaaaaaac
     5701 cggcgccgag gaaaacgcgc gcgccgctgt ggtcgcgggt tgaccggctc agcgcctgcg
     5761 ccagatgccg ccgccgctgt tgtagtagcc gtcgcggtaa ccggcgtaga aattggcgcg
     5821 gtgcttttca ctgacacgac tgcggtagcg cgccgggtag tagttcaggc cgcgcatgcg
     5881 gtccgagcgg ccgtagtcat agcccgtctg ccagacgccg acgccataac cgggggattg
     5941 ccagcccggc tgatgatggc cgggatgaaa cgggcggttg tagcccagcg gctgcccgcc
     6001 aaaaccgggg ccgggcggca gggtggccat gtaacaggaa ctcagggcga gggcgcaggt
     6061 cagtgtgaag agggaaagga gtgccgcttt catgcttcat gctggcataa tccgggtgtt
     6121 tgtccatgct cttgatacac gcggacgaag ccagcatcgc acgacatgga catcctgatc
     6181 accaatgacg acggcataga cgctcccggc cttgccgccc tcgagcgcgc cgcctgcggc
     6241 atgggccgtg tggttgtggt ggcgccgatg cgggagcagt ccatgtgcgg gcaccgggtg
     6301 acgacccgct cgcccatccg catggaacag cgctcgcagg accgatgggc ggtggacggc
     6361 acgcccgcgg attgcgtgcg tctggccctg cgcgcgctgg atttgcgtcc ggcctgggtg
     6421 ctttctggcg tcaatgcggg cggcaacatg gggcaggaca ttgtcatttc tggaactgtg
     6481 gcggcggcgc gcgaggcggc ctaccacggc ctccgcgcca tggcgctttc gcattatctc
     6541 attcgtgatc tcgccgtgga ctggctgagg gttggcgagt gggcgggcga gttgatgaga
     6601 gaactgtccg gcgcggctct ggcggacggc gagttttgga atgtgaacct gccgcaccat
     6661 ccgcccggca cgcttggcct gccggagcgt ttgccatgcc accccgcgcg cagcccgctg
     6721 aatgtcagct acgcgcccac cgaacacggg catgtttaca cggcttcgta tgcggagcgt
     6781 ccgcaggatc cgggttcgga tgtggaggtg tgtttttccg ggcgtgtggc ggtgtcgagg
     6841 ctgcgcatag ggtgacgaat tctttgcttg cggcatccgc ccgggttgtg gcttgattga
     6901 ggcgagccgg ttaaaccctt accccatcca tcccatgttc atagcttttt ggaatctgga
     6961 gaatctcttc gacattgaga cgagcaccga gcgccaggac tggctcaaaa tccgcctggg
     7021 caaggagttg cagggctgga cggcggcggt gctggaagcc aagctcgacc agttggcggc
     7081 tggcatcaag gccatgaaca gcggcgccgg gccggatgtg ctcggcgtgt gcgaggtgga
     7141 aaaccggcct gtgctggaga agctcgcgcg caagctcaac ctgcccggca ggagctacaa
     7201 ggtggcccat gaaaactcgc gtgacgggcg cggcattgac gtggcggtca tctatgacgg
     7261 caaacttttc acagttgacg gcaagtggtc gcactgggtc atgcgccgca ccggcacgcg
     7321 cgatcttttg caggtgaacc tcaagacgcg tgacgcggcg gctgagacac tggtggtcat
     7381 cgtcaaccac tggccctcgc gcagcggtgg cgagcacgcc tcggaaccct accggatgct
     7441 ggcgggggag actctggcct actggcactc gcgcatctgg gaggtgctgg gcaaggacgc
     7501 gctgatcgtg gcgatggggg acttcaatga cgagcctttc aaccgctccc tcaccgatta
     7561 cgcgctggct gaccgcgagc gccagaaggt gctgcgggcg gcctccgcgc cgcgtttcct
     7621 gaatctcatg ctgccgctgc tggctgccga gggcagctat gtcttcgacg ggcgcaaaaa
     7681 catgctcgac cagtttctgg tctcgcgcgg cctgctcaac gccacgggca ggttccgcct
     7741 gcgcgacgtc gcggtgagca tcatccgccc gccgggtgtg accggagcgc cggtgcggtt
     7801 cagccggccg tcagaaacgg gcggtgtgaa cacggcgggt tacagcgatc actttgccat
     7861 cgggttgaag ctgggctgat ccgggcacat gcggcgcgcc cgccatgcca gccagccgcc
     7921 gaggactccg ccgcaaacgt cagccagcca gtcgccgggg tcatttccca tgcggcccgg
     7981 cgtgaagctc tggtgcagtt cgtcgagcgc gcctgtgagc gcggtgaaga tgaagcccgc
     8041 cagcagccag gcccatgaag gcgggctctc cagccgcgcg gcgaaaagcg ccgtggccag
     8101 gcagaaggcg ccgccgctga agtaggcgca gtgcagcact ttgtccttga aggggaactc
     8161 cggcccggag ggcagcgcgc tgcgggagga gagccaccag agcaggcccg cccagagcaa
     8221 aatgccggcc caccaaagcc agcgggtcgt cagcagcttc gtcatcacgc ccggcgggcc
     8281 ggggatcatt cgcgcgtctc ctgctccatg cgctcgctga gccattgctt gatcatgctc
     8341 tggtagttga gataccggcg ccgggcgatg cgcttgatgc ggctgagcat gcgggggtcg
     8401 aagcgcagcg tgatggtggt ggactcgcgc acatcgttgc ggtggatgga catctgcatg
     8461 agccgcgggt ccacctggtg cgacgcccaa aatctgccct ccgcatcctc agagctgaag
     8521 gcgggcacat ccccccaggc gcgcacatag gcgagttcat cgcccggacg cgagctttgc
     8581 gtggtgtctc ctcggcgttt gggttccatg ctcatcaaac gtcctccgcg cgcttcctct
     8641 caaagaaatc cgcctccgtg ggtgtcatgt cccgcgcaaa gatgacgcgg tagcgttttc
     8701 cgtccgtcca aaaaacgctg aaaatgccgc gtccgccgag ggatttgccc aaattgtaat
     8761 agcgggcctc cgcgccttca ctgttgtcgt ccggcagcag cttcatggag aagggatcct
     8821 cgaacgactc ctcaatgtcc ttgggagtca gctttgtgag gtcaaaagtc actccgatcc
     8881 agtcgaaatc catgatgtgt agaattgcgg gttgccgccg cggcggcatt attggcgata
     8941 ttcgatcagt tctatctcat agccctccgg cgcgtcaatg aaagcgaacc tgccgctgga
     9001 actttccgtc ggtccgtcgg agagcgggta gcctttggcc tccgcgtggc gggcgaacgc
     9061 ttcgatatcc tccacctcga aagccaggtg agtcagatcc tggcacatcg tcacgggacc
     9121 gctggccggg aagctgcata tttcgatgag ctcgtcgctg ttgggtgtct tgaagaagac
     9181 cagctccgag ccgcgcggcg acttgtgcct tttgatctcc tccaggccca gcacctcctt
     9241 ataaaaggcg gcggttttct ccagatcgtt cacgcggtag cgggtgtgca gcagtttttt
     9301 gaccatggtt tggcggggat gtggctcatt gtgggacagt ctgcccgccc tggcaaccca
     9361 cagatcaaaa gccgccgccc cggcctgcgg gctgcatgtt tcttttctct ccgcgctggc
     9421 cccggccttg ctgttctgcc gcattgtgat tacccttgct catgaacact cgtttcgaac
     9481 ccctcgccgg actgaccgtc actgtggatg atgtgcatta cgatccgtcc cggcctgcgc
     9541 cgcctgaccg tccgcaccct tttctctatc atgtctcaat ccataacggc tcgccggaga
     9601 cggtgagcat cttcgggcgc aagtggatcg tgcgcgacac ggacggggac acactggtgg
     9661 tcgagggtga cggcgtcgtg ggccagttcc ccaaattgga gccgggcgag accttcagct
     9721 acaactccta ccacgtcatc aagtcggaaa gcacggccac aggctccttt ttcggcacca
     9781 cgggcgaagg ccgcccggtc tgcgcgcgca tcccgctttt tcagatgcag ccgccgctga
     9841 tggcatgaga aaggagcaca gacactcctg ttcgcagtct tgctgctact ggaggaagtg
     9901 cccttgactg ctctttactt ggcagcaccg aagtagtggc tgagggcctc catcatgcct
     9961 tcgctggcgt tttttcctgc cacgaaaccg ccgtggccaa gcacatgcgc cttcacggcg
    10021 ggcagggcgt tccccggcgt ggcgatcatg cccgcgtggc gcggatggag catggagagg
    10081 tcgttgtgat tgtcaccggc tgcgaagcat cgcgtggagt cgagatcaag caggcgcgcc
    10141 agttcggaga gcgcggtgcc cttgctgtga gcgctgtgcg aaaagcgcag gtagatgcca
    10201 ttgcggtggt agccgatgtc ggggaaacgc gcggcttgac tgtctatgaa ggcgcagatg
    10261 cggtccatct cttcgtcact agcggccacg atgcccagtt cgccatactc tccctccaga
    10321 aagttcgccg ctgtctgggt ctcgaccatt tcctggatgg agtcgaggga tgcctggtgg
    10381 tctttgacga agcgttcatg ggctttgcgc gcctggcggt tccaggggcc gaagtccttc
    10441 cagccgccca gcatgccggg ccggtaaata tcgcactcca tcgcgatgat gtagtccggt
    10501 tccatgaaga tgccgtattg cgccagcccc tgcagggtat gggagaggct gcgcccggtg
    10561 ttgatgaccc aggccgcccc ctgggcgcgc agttgctgga tcatgcggcc gagtccggga
    10621 tgaaacaccg gatcgctccc cggatgcacg agggtgccgt cgaagtcgaa ggagaggatg
    10681 aagctggtct tgcgggcggc catgcgcgcg catgtttccg cgcggcccga tgcgccgtca
    10741 agcgggcgtg cttgcctcgg cgccggcctg gtcagcgggt gcggtaggcg tcgttgccgt
    10801 tttgctggaa ataggcgtcc cgcaggcggc gcagttcatc gaggcgggag agggactcgc
    10861 tctgctggcg cgccttctgc tcctcctggg cgcggacgaa gggattgttc gcgcccagcg
    10921 tgcgccaggg tccgggcgga acctcgctgg tgtccacgaa acgccagtcg cacctcgctt
    10981 tgctggggat gccgaggtag tcgcggactg cgggtgagat gtcaatgccg gcgccgctgt
    11041 tgctggtgtt cttcgggcgc gcgctgccga agacgtattc atggtcgtcg gtgacaaacg
    11101 ggccgcagtc ctcccactgg gcataacaca cgcggttgcc gtagcggatg gccacccagg
    11161 tgcctttgag cacggacttg ccggagcgca cgaagcgatc tttgaaccac gggatgactt
    11221 tttcagcctc ggacttgtgt gccttgtggt cctggcagtc gttgtagggc agggcgatgt
    11281 aaaagggatt ttggcccggc acaaattcgg ccgggcaata attggcgttt cttttggacg
    11341 gatcgggatc gtcataaccg ccgaagtttt tctcccattc cgtgtcccag gagcttttgt
    11401 tgttgggggt ggggttgttt tccgcgggtt tttcccccac ccaaaagact gtggccgtga
    11461 tgtcggtctt ccacggataa cgggcataac ccagtctggg catggtgggc acagtgactc
    11521 tgggacccgt ggtttgcggc agggagttct ggcctgactc agcccgacct tgaaaggcgg
    11581 caacgctggc tgtgctggtc accttcggcg cggactgacc gcgtgacatc cccgcgcccc
    11641 ctgccaggat gaggaggagg acggacgaca tcacgagggg cgggcgtgtg tggaccatgc
    11701 gggtttgcat tttccgtaat ttaccagacg gccggacttt tgagcaactg gaaatccggc
    11761 aatctttggc cgttataaca accctgtgtc atgcagccgg gattcggccg gagggaaaat
    11821 gggccctttt ctcagatcat gtattccatc atcaccggag tctcggcagc cggtgtggac
    11881 ttctggccga ggattgtctc ggccactctc aacgcttgtc cgcgcaattc cggcacagcg
    11941 atggtttccc aatatgtgcc gcgaagaagc gggccgagag cgagaaggaa tggggatacc
    12001 ccgccatccc ggtcaatgac ggtgtgactg ccgtcaacac gcgcgcccat gtccatgtcg
    12061 tcgctgctga tgaggccccg ccgcagcaga tttcgcagca gcacggagcg cgtcgaactg
    12121 aagcgtgtcc tcggacccgt ggcattgatg acgagtccgc cttcaagctg cccgcctcct
    12181 tcgaaatgca cgcgcacgcg cgctccggcc gcctccacat gctcaatgtt ctccgcgtgg
    12241 acctgaagct ggccggtgag ctgggcgctg gtgatttggg cgtgaatctc aggagcgatg
    12301 cggtgacgca ggacattcca cctcgcggca tgctggcggg tgaaggcgag ccgttgctca
    12361 aggctgaaac cctgccagat gcgctgtgtg tgcgggcgca ttttatccac gataattgcc
    12421 gggttcgccc ccattgcccg cagccgggcg cagtggtcct ccatgagcgc ggcgagcctg
    12481 tccagtccga gtgcggcgag gtccgtgtcg gctggaggaa agtccgggta ttcaacgccg
    12541 cgaaagtgcg cgtgcggcag ccagccatga cgcgagaccg cgtggatgcg tccgagccac
    12601 cccagagcgc gcaaagtgat gatcgcgtcc actgtggtca ggcctgtgcc cagcaggacg
    12661 atgctttccg tgtctggcgg cagtctctca tgccatggca gccatggatt cccagcccag
    12721 gctggatttt cctggagtga ttccgctccc ggcaggtcgg ctggagcctc atttccagtg
    12781 gccaggacga cc
//
LOCUS       DAOMYJ010000152        12659 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Prosthecobacter sp. isolate AMR_MDS_5629
            NODE_1663_length_12659_cov_18.841400, whole genome shotgun
            sequence.
ACCESSION   DAOMYJ010000152 DAOMYJ010000000
VERSION     DAOMYJ010000152.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867850
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prosthecobacter sp. (wastewater metagenome)
  ORGANISM  Prosthecobacter sp.
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Verrucomicrobiaceae; Prosthecobacter.
REFERENCE   1  (bases 1 to 12659)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12659)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.30
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:34:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,742
            CDSs (total)                      :: 4,684
            Genes (coding)                    :: 4,676
            CDSs (with protein)               :: 4,676
            Genes (RNA)                       :: 58
            tRNAs                             :: 55
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12659
                     /organism="Prosthecobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1663_length_12659_cov_18.841400"
                     /isolate="AMR_MDS_5629"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1965333"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2286)
                     /locus_tag="PLP58_14935"
     CDS             complement(<1..2286)
                     /locus_tag="PLP58_14935"
                     /inference="COORDINATES: protein motif:HMM:NF019219.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PSD1 and planctomycete cytochrome C
                     domain-containing protein"
                     /protein_id="HRJ09870.1"
                     /translation="MYSHPLLFFPPLLLLSATAAAIEPESLKFFENKVRPLLAEACYE
                     CHADKKQKGGLRVDNLAYLRQGGESGPAVVAHQPDASLLIKAVSYADPDLEMPPDGKL
                     ADEKIAILKQWIAMGAPWPEAEVASAKPARKPGEFTEEDRAWWAFQPVKRPPLPAVRD
                     PQYKIRNPIDAFILAKLEENGLTPAPEASPEELARRVYFDLHGLPPTPEEIAEFTAAI
                     EADKSAPKAKGPGAYERLIDKLLASPRYGERWAQHWLDLARYAESEGYRLDAYRPNAW
                     PYRDYVIESFNSDKPYDQFVREQIAGDELDPQNPKVTIGTAFLRHTIYEYNQRDAETQ
                     WQNILHEVTGVTADVFMGMSVQCAQCHDHKFDPILQDDYYRLQAFFSNITWPEDKPLS
                     APEEIRAWNARDAAWRAAAKEPLAVIDRIIEPRVAAAQRNAMEKFPDEVQAMWRKPRE
                     QRTAYEEQVVQLAWRQADYERVRFKEDKIKEPELSELRAAREALAQFDHLKPDPLLTA
                     FVIGETGGKPGETRFKGRRTGDTTVNPGFLTILDPTEAQIPEVSPGATTTGRRKVLAD
                     WLTRPDNPLTARVIVNRVWQYHFGRGIAATPSDFGRLGEAPTHPELLDWLADDFVTGG
                     WKFKALHKKILLSAAYRQTARREPGAKELTTDPENRLLWRFNPRRLDAEQARDAVLAA
                     TGELDPAMGGEGVTAGKPRRSIYTRKIRNTQDEFLASLDAPPGFASAPSRDSTTTATQ
                     SLLMINGDWPLERARAMAARLA"
     gene            complement(2356..3084)
                     /locus_tag="PLP58_14940"
     CDS             complement(2356..3084)
                     /locus_tag="PLP58_14940"
                     /inference="COORDINATES: protein motif:HMM:NF022142.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2490 domain-containing protein"
                     /protein_id="HRJ09871.1"
                     /translation="MKYPAVVLFLLAICEATASARDQHADNHNLWLNYVGDHPLSGSP
                     WGLHLEAQVRREDWGDEWQQLLLRPGVNYQISPTLSASAGWAYVRTYPYGELPVAHEF
                     DEHRAWEQVSWRTEMLGLEWQHRFRLEQRWLEEQDARGSRLDWRLENRFRYMLRTSVP
                     LTANKKTYLALWNEVFLNFGGNIDKNHFDQNRAFIGIGRKLSDHTRIEIGFLEQTVQR
                     RGGDKWENNHTASVWLMSAWPFGR"
     gene            3261..3926
                     /locus_tag="PLP58_14945"
     CDS             3261..3926
                     /locus_tag="PLP58_14945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ09872.1"
                     /translation="MKKYLALAALGLLASCTSAQLERRPSREAAYEGEKKLNEAFGTG
                     LHESIDRTGHVASDVVNDTAGMTGNFGRDQTRRYAGTTINATRYASNVVSRETRNTAD
                     TVLDITDDTVDLGNEQVSTYTGIVNKSAGRLMCTSGKIVGTGVETYANTTHTLFGGIV
                     CLFKRTVVDTKPFMVGSANDQDRCWKNVAMPGESWNRPFPESPQYVVAPAPTSGKQVT
                     FSK"
     gene            complement(4094..5020)
                     /locus_tag="PLP58_14950"
     CDS             complement(4094..5020)
                     /locus_tag="PLP58_14950"
                     /EC_number="4.1.3.3"
                     /EC_number="4.2.1.41"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: protein motif:HMM:NF012904.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009436 - glyoxylate catabolic process
                     [Evidence IEA];
                     GO_process: GO:0071704 - organic substance metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HRJ09873.1"
                     /translation="MAPNFKPPLRGVLPVFQTPWLDNETLDLDTLEREIAWLYDSGSN
                     GIVMAMVSETLRLDSEEREQLAAAACRFGKKRGVVVISVGAESSKVAERYAKHAESVG
                     ADAVMAIPPVSIGIGENELLAYYTRIIEAIRIPVIVQDASGYVGKPMPIAMQARLLDE
                     FGPERVQYKPEASPIGPKLSELRDATKGRARVFEGTGGLALVDSFKRGVVGTMPGADL
                     IRGLVPLWKALEAGDTAKADAIHGPLSALISMQTSLDGFLAVEKHLLKRQGIFKNTLV
                     RGPVGFKLDAETTREVERQFDRMIAAACPASR"
     gene            5152..>5523
                     /locus_tag="PLP58_14955"
     CDS             5152..>5523
                     /locus_tag="PLP58_14955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ09874.1"
                     /translation="MSHSAASAPVRRSLLVTLLGWMVILGSAALSVISFFSFLMILAG
                     SYGTQNTTLSGFLLIVVAPPASLVAGIGLLRRWRWAWVYLVAGLLLAIGWQVLEGMRP
                     AKPAVTTTVLPSGVKSTVYHSG"
     assembly_gap    5524..5623
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            5781..6413
                     /locus_tag="PLP58_14960"
     CDS             5781..6413
                     /locus_tag="PLP58_14960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ09875.1"
                     /translation="MPNAFQTPKAETAALDHERGWRVGHRGRDCMFYEELHAGAWQRL
                     DISGEMLTGRAHHVIYFPTAKAWSQMPEWARDRREEIIARIKSEFREPDYEYADDVDV
                     TAVPAGAVVANDVNVTASAGQKGGLLGMLLALLAVVAFMGWQVKGGFENGQIRLPVKG
                     GLRRTVVREAEPALFWTCMGIHLVVGGGSLGLAVWLAAQGLKTRRPGGLR"
     gene            6421..7404
                     /locus_tag="PLP58_14965"
     CDS             6421..7404
                     /locus_tag="PLP58_14965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ09876.1"
                     /translation="MNALRRHQLNHPPPAEKVLEERRVDCGCDERLHRPVLLEPGFQA
                     WAVHACCGCGAVTCTEQRGDDGRFTGEAWSVHVALMLKPEVMTWLASWARLGPHEREV
                     LWPMPAGWVRRGHRYLPAGWSGFSVEDLERREAALHEEQADLGVRQRLLLTGVPSEPP
                     PAALPPQLAGFAVVWQAMQLTPETDTKVLLPYAQGSGPGSAIAAELLTGMQDAPQRLV
                     ELLRSGRAGPLQAALALLRAAPRPECLPLILEALQAVPLTPLSDVPDRLSHWDCFELL
                     LLMLAELRTQASEAPAVLRALMRKVARHDTTLVDRLRLVTALLEFKAPPQV"
     gene            7420..7800
                     /locus_tag="PLP58_14970"
     CDS             7420..7800
                     /locus_tag="PLP58_14970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ09877.1"
                     /translation="MPASSDSFPSQVLAALRAKRGPQGDFIEMLRTGRVLLVWTGLTR
                     EGCSARRAATGLDWHFPAITPPPPAPLLPALQPLAAVFAPLPAKLAGIVLMLVFYVGW
                     FFKGAAWAASAPPAAGTGRPGCAG"
     gene            complement(8075..8854)
                     /locus_tag="PLP58_14975"
     CDS             complement(8075..8854)
                     /locus_tag="PLP58_14975"
                     /inference="COORDINATES: protein motif:HMM:NF024950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HRJ09878.1"
                     /translation="MTILDQFNLSGRRALITGGSRGLGLEMARALGEAGAHVIITGSN
                     AENLHKACEELCSEDLHVSSIQVDLSRPDQAGRLCDVVLEEHAPVDILINNVGGRRIN
                     TPTEDLALDDWQRILDLNLTQAFILTKRLGGAMIPRKWGRIINITSINALWPGKAMRG
                     RSYEASKGALTMFTKAVAADWAVHGITVNAIAPGPFLTDANKRWIGERPDFEDEVAAS
                     IPMGRWGRPEEIGPLALYLASEAGSFMTGSVLVLDGGKLLW"
     gene            complement(8937..10781)
                     /locus_tag="PLP58_14980"
     CDS             complement(8937..10781)
                     /locus_tag="PLP58_14980"
                     /inference="COORDINATES: protein motif:HMM:TIGR02226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HRJ09879.1"
                     /translation="MRFLAPHLLHLAWLALIPLALYLFKKKARRVPVSTLLFFRSLAR
                     EHQESAWLRKIKKWLSLLLTLLMLFLIVLALARPVGEAGADSPGAVVLLMDRSASMAA
                     RDEKGRTRLEEARQLARAKLKSLPEQVVVSLVACDAKAHVLLSRSRNRRECLRLLDEL
                     APRPLTLRPDIALITAARLAELEPGTQVWHFGDRAFEAGAELAYQFHSAALDAPLNAG
                     ITAFQIRPAPLAHDRYEAFVKVTAAPANARRVTSTLEVTLAGRLAQLRELELEPGESA
                     ALILPLEGVKGQRMTVQIKTPGDCLGWDDAVAAVLPRSRPLVVAWVAENADPFTELAL
                     TSMIEAGRIEMLRGTPAAWPMSDKPDVYVFEQWLPDPWPADRPAIVLNPPKSAGPVQV
                     RRLTGAGLPHESVRSAAPEHPVLFRVSSSRLAITQTGVLGTGTSLEPLWMAGSEPVLA
                     AGEVEGQRLVISAFSPARSEQLALLPAFPLMLGNALYWCAEQAAALAEIQTRHPGDLL
                     EEEGLLRWTEWDGTRFLETTEVLQGGVTAITRLGAWENGDDRTGVSALASAAETSVPA
                     RATEDPASASLPAFAAGPVFRDWPAILTASLLFLLLLESWLFHRKAVY"
     gene            complement(10797..11711)
                     /locus_tag="PLP58_14985"
     CDS             complement(10797..11711)
                     /locus_tag="PLP58_14985"
                     /inference="COORDINATES: protein motif:HMM:NF024909.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HRJ09880.1"
                     /translation="MSQAALEREDLFDAAFLDRLRSLAVRLRKRRALSRRGAQSTPAT
                     GFTREFKDYRHYTPREDYRAIDWRLYARLDKLFVRLYEETQELNLHVLVDTSASMARP
                     FGEKRRQALRFAVALAYLGLAAQQRVSLYSLSDTVRQELPPLRGQGNIEKIIQAATRL
                     DFGGLTDLERGFTEFRPTRQRYGVIFVLSDFFGRDVNTASHAIQRAAAWPGETHFVQI
                     LHPEEREPRIEGEVELDEVETGERRRFWLTRRDVQRYSETFDAFCDTLARECAAHRMD
                     FMQCGATEPFEDRFLDLLNRGSALAGGV"
     gene            complement(11708..12373)
                     /locus_tag="PLP58_14990"
     CDS             complement(11708..12373)
                     /locus_tag="PLP58_14990"
                     /inference="COORDINATES: protein motif:HMM:NF017496.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Uma2 family endonuclease"
                     /protein_id="HRJ09881.1"
                     /translation="MPAVAIKLNAPSVHTPPKPPRITEAEYEAILAKSEDKLEFRDGR
                     IIAMAGGSPSHSLVKSNLGHCFKGALAGRGCRVFDSDMKVKVEATGLNTFPDLSIVCH
                     KPRFTANGLTLLNPGLIAEALSPATEAYDRGEKFWHYRHLPELRCYVLVSTAQALVEV
                     YERQADDSWRLTSFDGWQAVMRLEELRVEIPLSGIYALTALDPDQPLDETADVADEEA
                     PQA"
     gene            complement(12452..>12659)
                     /locus_tag="PLP58_14995"
     CDS             complement(12452..>12659)
                     /locus_tag="PLP58_14995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009962673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ09882.1"
                     /translation="PAIVWILAIWAAVHGATAVSTEDVRALAHVALRHRVILNFEGEA
                     QQVKVDDIITKLLDQIKTPAQEAA"
BASE COUNT         2016 a         3871 c         4385 g         2287 t
ORIGIN      
        1 ggcgagcctg gccgccatgg cgcgggcgcg ttccaggggc cagtcgccat tgatcatgag
       61 caggctctgg gtggctgtgg tggtggagtc acgcgaaggc gcgctggcga agccgggcgg
      121 cgcgtccagg ctggcgagga actcgtcctg ggtgttacgg attttccgag tgtaaatgct
      181 gcggcgcggt ttgccggcgg tgacaccttc cccgcccatg gcggggtcca gttcgccggt
      241 ggcggcgagc acggcatcgc gcgcctgctc ggcgtcgagg cggcgtggat tgaagcgcca
      301 gaggaggcgg ttttccgggt cggtggtcag ctctttggcg cccggctcgc ggcgcgcggt
      361 ctggcggtag gcggcggaaa gaaggatctt tttgtgcagg gccttgaact tccagccgcc
      421 ggtgacgaag tcgtccgcca gccagtcgag cagttcggga tgagtgggcg cttcgccgag
      481 ccggccgaag tcgctcggtg tggcggcgat gccccggcca aagtggtatt gccagacgcg
      541 gttgacgatg acgcgggcgg tgagtggatt gtccgggcgg gtgagccagt cggccagcac
      601 cttgcgccgc ccggtggtgg tcgcgccggg agagacttcg gggatttgcg cctctgttgg
      661 gtcaaggatg gtgaggaagc cgggattgac ggtggtgtcg cctgtgcggc ggcctttgaa
      721 tcgcgtctct ccgggcttgc cgccggtctc gccgatgacg aaggcggtga gcaggggatc
      781 gggcttgagg tggtcgaact gcgcgagcgc ctcgcgcgcg gccctcagtt cggagagctc
      841 gggttccttg atcttgtcct ccttgaagcg cacgcgctcg tagtctgcct ggcgccaggc
      901 gagctggacc acctgctcct cgtaggcggt gcgctgctcg cgcggcttgc gccacatggc
      961 ctgcacctcg tcggggaatt tctccatggc gtttctttgc gcggcggcga cgcgcggctc
     1021 gatgatgcgg tctatgacgg ccagcggctc tttggccgcc gcgcgccagg cggcgtcccg
     1081 cgcgttccag gcgcggattt cctccggcgc ggacaggggc ttgtcctcgg gccaggtgat
     1141 gttcgaaaag aaggcctgga ggcggtagta atcgtcctgg aggatggggt cgaacttgtg
     1201 gtcgtggcat tgcgcgcact gcacgctcat gcccatgaac acatccgctg tgacgccggt
     1261 gacctcgtgc aggatgttct gccactgggt ctccgcatca cgctggttgt actcgtagat
     1321 ggtgtggcgg agaaatgcgg tgccgatggt gactttcggg ttctgcgggt ccagctcgtc
     1381 gcccgcgatt tgctcgcgca cgaactggtc gtagggtttg tcgctgttga aggactcgat
     1441 gacgtagtcg cggtagggcc aggcattcgg acgataggcg tcgaggcggt agccctcgga
     1501 ctcggcatag cgggcgaggt ccagccagtg ctgcgcccag cgctcgccgt agcgcgggga
     1561 ggcgagcagc ttatcaatga ggcgctcgta ggcgccgggg cctttggcct tgggcgcgga
     1621 tttgtccgcc tcaatggccg cagtgaactc cgctatttcc tctggcgtgg gaggcaggcc
     1681 gtgcaggtca aaatagactc tccgggccag ctcctccggg gacgcctccg gggcgggagt
     1741 cagcccgttt tcctccagct tggcgaggat gaaggcgtct atgggattgc ggattttgta
     1801 ttgcggatcg cggactgcgg gcagcggcgg tcttttcact ggctggaagg cccaccaggc
     1861 gcggtcttcc tcggtgaact ccccgggctt gcgcgcgggc ttggcggagg cgacctcggc
     1921 ctcgggccag ggcgcgccca tggcgatcca ttgtttgagg atggcgattt tttcgtcggc
     1981 gagtttgcca tcgggcggca tctccagatc cgggtccgcg taggagaccg ctttgatgag
     2041 cagggaggcg tccggctggt gcgccaccac ggcggggccg ctttcgccgc cttgcctgag
     2101 gtaggccaga ttgtccacgc gcaggccgcc tttctgcttt ttgtcggcgt ggcattcgta
     2161 gcaggcctcc gccaatagcg ggcgcacctt gttctcgaaa aacttcagcg attccggctc
     2221 aatggccgcc gcggtggcgg acaggagcaa aagcgggggg aaaaacagaa gcggatggga
     2281 gtacatgact gtggaataaa agtcgtcgca gggagccttt ttgacataaa atccgccgcg
     2341 cgcgcagggg ccggatcacc ttccgaaggg ccaggccgac atcagccaga cgctggcggt
     2401 gtggttgttc tcccatttgt caccgccgcg ccgctgcacg gtctgctcca gaaaaccgat
     2461 ctcgatccgt gtgtggtccg agagcttgcg gccgatgccg atgaaggcgc ggttctggtc
     2521 gaagtgattt ttgtcaatgt tcccgccgaa gttcaaaaac acctcgttcc agagggcgag
     2581 gtaggttttc ttgtttgccg tcaagggcac cgaggtgcgc agcatgtaac ggaaacggtt
     2641 ttccaggcgc cagtccagcc tgctgccgcg cgcgtcctgc tcctccagcc agcgttgctc
     2701 cagccggaaa cggtgctgcc actcgagtcc gagcatctcc gtcctccacg acacctgctc
     2761 ccaggcgcgg tgctcgtcga actcatgcgc cacaggcagc tccccgtatg gataggtgcg
     2821 cacataggcc cagccggcgc tggcgcttaa tgtggggctg atctggtaat tgacgcccgg
     2881 ccgcagcagc agttgctgcc attcatcccc ccagtcctcc cgccggactt gcgcctcaag
     2941 gtgcagaccc cagggggagc cggacagcgg gtgatccccc acatagttga gccagagatt
     3001 gtggttgtcc gcatgctggt cccgggcgct ggcggtggcc tcgcaaatgg cgaggaggaa
     3061 gaggacgacg gctgggtatt tcatggggcg ggaaaaaacg ccggacggca tcgaaatgta
     3121 ccaactgccg cgcggtggaa gccgccatgt gagacactca ccccaggcgc ggaccgcccg
     3181 cggaccaggc ggcaactttc agcaagtcca attttgtttt gacatcccgg cgtcaaacag
     3241 acaatctcgc ggcgctattt atgaaaaaat atctcgctct cgcagctctt ggacttcttg
     3301 catcctgcac ttccgcccaa ctggaacgcc gtccctcgcg cgaggcggct tacgagggcg
     3361 agaaaaaact caacgaagcc ttcggaaccg ggctgcacga gtccattgac cgcacaggcc
     3421 atgtggcatc cgacgtggtc aacgacaccg cgggcatgac cggcaatttc ggccgcgacc
     3481 aaacccgccg ttatgcgggc accaccatca acgccacccg ctacgccagc aatgtcgtct
     3541 cgcgcgagac ccgcaacacc gccgacaccg tcctcgacat cacggatgac acggtggatc
     3601 tcggcaacga gcaggtcagc acctacacgg gcatcgtcaa caaatccgca ggacgcctca
     3661 tgtgtacgag cggaaagatc gtcggcactg gcgttgagac ctacgccaac accactcaca
     3721 cccttttcgg cggcattgtc tgcctgttca agaggaccgt cgtggacacc aagcccttca
     3781 tggtcggcag tgcaaacgat caggaccgct gctggaaaaa cgtcgccatg cccggagaaa
     3841 gctggaatcg tccgttcccg gaatccccgc agtatgtggt cgcccccgcc cccaccagcg
     3901 gcaagcaggt caccttctcg aagtgaattg gaaacaagag catcgtcctt cttcagcggc
     3961 ttccctcggg aagccgcttt ttttgcgccg cataaacctg tcagtccgct cctttgcctg
     4021 tgtctccaaa catgcatgac accgggtgcg cggaccatgg gaggctgtcg gaagccgcgc
     4081 agcgcatccc gcattaccgg ctggccgggc aggcggccgc gatcatgcgg tcaaactgcc
     4141 gctccacctc gcgcgtcgtc tccgcgtcga gtttgaagcc caccggaccg cgcacgagcg
     4201 tgtttttgaa aatgccctgc cgtttaagca gatgcttctc caccgccaga aagccatcga
     4261 ggctcgtctg catcgaaatc agcgctgaca gcggcccgtg aatggcatcc gccttcgcgg
     4321 tgtcaccagc ctccagcgcc ttccaaagcg gaacgaggcc gcgaatcaaa tcggcgcccg
     4381 gcatcgtgcc caccacgccg cgtttgaacg aatccaccag cgcaaggccg cccgtgccct
     4441 caaacacacg ggcacgaccc ttcgtcgcat cgcgcagttc gctcagcttc ggcccgatgg
     4501 ggctcgcctc cggcttgtac tgcacgcgct ccggcccgaa ctcgtccagt agccgcgcct
     4561 gcatcgcgat gggcatcggt ttgcccacat agccgctcgc atcctgcacg atcaccggaa
     4621 tgcggatcgc ctcgatgatg cgcgtgtaat acgccagcaa ctcgttctcg ccgatcccga
     4681 tggagaccgg cggaatcgcc atcaccgcgt ccgcgccgac gctttccgca tgcttcgcat
     4741 accgctccgc gacctttgac gactccgcgc ccacgctgat caccaccaca ccgcgctttt
     4801 tgccaaaccg gcacgccgcc gccgccagtt gctcgcgctc ctcgctgtcc agccgcagcg
     4861 tctccgacac catcgccatc acgatcccat tcgaaccaga gtcgtaaagc cacgcgatct
     4921 cccgttcgag cgtgtcgagg tcgagcgtct cattgtccag ccagggtgtt tggaaaaccg
     4981 gcaggacgcc gcgaaggggg ggcttgaagt ttggagccat ggaaaaccga aggtgggaca
     5041 ataacaatca cagcgggtgg tttggtcagg aaatttcacg ttctgcgggc cggggtggcg
     5101 ctgcgcactt gatcttgcaa gccaacgctg gtgctccaca atccgctccg catgagccac
     5161 tctgccgcct ccgcaccagt ccgccgttct ctgctggtca ccctgctggg ctggatggtc
     5221 attctcggca gcgcggcgct ttcggtgatc agtttctttt cgttcctgat gatcctggcc
     5281 ggcagttacg ggacgcaaaa caccacgttg tccgggttcc tgctgatcgt ggtggcacca
     5341 cccgcgagcc tggtggcagg catcggactg ctgcggcgct ggcgctgggc ctgggtgtat
     5401 ctggtggcag gcctgttgct ggcaatcggc tggcaggtgc ttgaagggat gcgtccggcc
     5461 aagccggcgg tgaccaccac cgtgctgccg agcggggtga agagcacggt gtatcactcc
     5521 ggtnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5581 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnncaccgtg ctgccgagcg
     5641 gggtgaagag cacggtgtat cactccggtt cctcgttttc cttgccgttg accgcagtgc
     5701 tcggcggtgt gctggtcttt gcgctgacgc cgggcgtgcg gcgggagttt tgggtttagc
     5761 ggaggagcct tccaccccct gtgcccaatg catttcagac accaaaggcc gaaaccgcgg
     5821 cgctggacca tgaacgcggc tggcgtgtcg ggcatcgcgg ccgcgactgc atgttttatg
     5881 aggaacttca cgccggtgcg tggcagcggc tggacatcag cggtgagatg ctgaccgggc
     5941 gggcgcatca tgtgatctat tttcccaccg cgaaggcatg gtcgcagatg cccgagtggg
     6001 cccgggaccg tcgggaggag atcatcgccc gcatcaagag cgagttccgt gagcctgatt
     6061 atgaatacgc tgacgatgtg gacgtgactg cggttcctgc tggtgcggtt gttgccaatg
     6121 atgtgaatgt aactgcgtca gccggacaaa agggcggctt gctcggcatg ctgctggcct
     6181 tgctcgccgt ggtggcgttt atgggctggc aggtcaaggg tggtttcgag aatggacaga
     6241 tccggcttcc agttaaaggc gggcttcgtc gcacggtggt gcgtgaggca gagcccgcgc
     6301 tgttttggac ctgcatgggc attcatctgg ttgttggcgg cggctctctt ggtctggcgg
     6361 tctggctggc ggctcaggga ttgaaaacgc gtcgcccagg aggtctccga taagacgatc
     6421 atgaatgcgc tccgtcggca tcaactgaac catccgccac cggcggaaaa ggtgctggaa
     6481 gagcgccggg tggactgcgg ctgtgatgaa cggctgcacc gccccgtgct gctggagccg
     6541 ggtttccagg cgtgggccgt gcatgcctgc tgcggctgcg gcgcggtgac ctgcaccgaa
     6601 cagcgcggcg atgacggccg cttcacgggc gaggcatgga gtgtgcatgt cgctctcatg
     6661 ctcaagccgg aggtgatgac ctggctggca tcctgggcgc ggcttgggcc gcatgaacgg
     6721 gaggttttat ggccgatgcc tgccggctgg gtgcgtcgag ggcatcgcta tctgcccgct
     6781 ggctggagcg gcttctcggt ggaggacttg gagaggcgtg aagcggcgct gcatgaggaa
     6841 caggcggatc tcggtgtccg ccagcggctg ctgctgacgg gcgttccatc ggaaccgcct
     6901 cccgcggccc tgccgccgca gctcgcgggc tttgccgtgg tctggcaggc gatgcagctc
     6961 acgccggaaa cggacacgaa ggtgctgttg ccatacgctc agggcagcgg gcccggcagc
     7021 gccatcgcgg cggagttgct gacggggatg caagatgcgc cccagcgact ggtggagctt
     7081 ctgcgcagcg gccgagcggg tccgcttcag gcggcgctgg ccctgctgcg tgcggcacca
     7141 cgcccggagt gcctgccgct gatcctggag gcgctgcagg cagtgccgct gacaccgctg
     7201 tcggacgtgc cggaccgcct ctcccactgg gactgctttg agctgctgct gctcatgctc
     7261 gccgagttgc ggacgcaggc aagcgaggca cccgccgtgc tgcgggcgct catgcgcaag
     7321 gtggcccggc atgacaccac cctggtggat cgcctgcgcc tcgtgaccgc cctgctcgaa
     7381 ttcaaagcgc cgcctcaagt ttgaaccccc ctccatcgca tgcccgcctc ctccgactct
     7441 tttcccagcc aggtgctcgc ggccttgcgg gccaagcgcg ggccgcaggg agatttcatc
     7501 gagatgctgc gcaccgggcg tgtgctcctg gtgtggaccg gactgactcg tgagggctgc
     7561 tccgcccgtc gtgcggccac cgggctcgat tggcactttc cagccatcac gcccccgccg
     7621 cccgcaccac tcttgcccgc ccttcagccg ctcgccgccg tctttgctcc gctgccggcc
     7681 aaactggcgg gcatcgtgct gatgctcgtt ttttatgtgg gctggttttt caaaggcgcg
     7741 gcatgggccg ccagtgctcc gcctgctgcc ggcacgggcc gacctggttg cgctggttga
     7801 cggagtagcg tgttgcacgc ctttcgagac ggcgaggatc gcgagacgct ctgccgcgag
     7861 ctcttcgccc gcctcggcag ccgggatccg tgaacgcatc ccctccatgc ggtccacagt
     7921 cgcccccttt ccattcgtcc gcagactccg ggtgacgttt cgccgcggaa accgtcccca
     7981 ggccatcctc atcttgaaag cgctccagct cctcatcctc ggcgtgctgc aatgcctggc
     8041 tgatgagcct gccagaaagc cagccatttg gacttcacca cagcagcttc ccgccatcca
     8101 gcaccaaaac actgccggtc atgaagctgc cggcctcgct ggccagatac agggccagcg
     8161 ggccgatctc ctccggcctg ccccagcggc ccatcgggat gctggcggcc acttcgtcct
     8221 cgaaatcggg cctttcgccg atccagcgct tgttcgcatc cgtcaggaac ggaccgggcg
     8281 cgatggcgtt cacggtgatg ccatgcacgg cccaatcagc agccacggcc ttcgtgaaca
     8341 tggtgagagc acctttggag gcctcgtagc ttcggcctcg catcgccttg ccgggccaga
     8401 gggcgttgat ggaggtgatg ttgatgatgc ggccccactt gcgcgggatc atcgcgccgc
     8461 cgaggcgctt cgtgagaatg aaggcctgcg tgagattcag gtcgaggatg cgctgccagt
     8521 catcgagcgc cagatcctcc gtcggcgtgt tgatgcggcg gccgccgacg ttgttgatga
     8581 ggatgtcaac gggcgcatgc tcctccagca ccacatcgca cagcctgccc gcttgatcag
     8641 gccgcgagag atccacctgg atcgaagaga cgtgcaaatc ctcgctgcac agctcctcgc
     8701 aggccttgtg caggttttcc gcgttggagc ctgtgatgat gacatgcgcc cccgcctcgc
     8761 ccagggcgcg tgccatctcc aggccgagcc cgcgcgagcc tccggtgatg agcgcgcggc
     8821 gtccggaaag attgaattgg tcgagaatgg tcatggcgag aagtgcttgg aaaaaatcga
     8881 atgtccgcag tgtccaatgc gggccatgcg gtggcttggc cgcttgcttg aacctttcaa
     8941 tacaccgcct tgcggtgaaa gagccagctc tccagcagga gcaggaacag caggctggcg
     9001 gtgaggatgg cgggccagtc gcggaagacg gggccggcgg cgaaggcggg cagtgatgcg
     9061 gaggcggggt cctctgtggc gcgcgcgggg acgctggtct cggcggcgga ggctagggcg
     9121 ctgacgccgg tgcggtcgtc gccgttttcc caggcgccga ggcgggtgat ggcggtgacg
     9181 ccgccctgga gcacttcggt ggtttcgagg aagcgggtgc cgtcccactc ggtccagcgc
     9241 agcaggcctt cttcttcgag gagatcgccg gggtggcggg tctggatttc ggcgagggct
     9301 gcggcttgct cggcgcacca gtagagggcg ttccccagca tgagggggaa ggcgggcagg
     9361 agggcgagct gctcgctgcg cgcgggggaa aaggcgctga tgacgaggcg ctggccctcc
     9421 acttctccgg cggcgagcac gggctcgctg ccagccatcc agagcggttc caaagacgtg
     9481 ccggtgccga gcacgccggt ctgggtgatg gcgaggcggc tgctggagac gcggaaaagc
     9541 accgggtgct cgggcgcggc gctgcgcacg ctttcatgcg gcaggcccgc gccggtgagg
     9601 cggcgcactt gcacggggcc ggcggatttg ggcggattga gcacgatggc ggggcggtcg
     9661 gcgggccagg ggtcgggcag ccattgctcg aagacataga cgtcgggctt gtcgctcatg
     9721 ggccaggcgg cgggtgtgcc gcgcagcatt tcgatgcgcc cggcttcgat catggaggtg
     9781 agggcgagct cggtgaaagg gtcggcgttt tccgccaccc aggcgacgac gagcgggcgc
     9841 gagcgcggca gcacggcggc cacggcatcg tcccagccga ggcagtcgcc gggggtcttg
     9901 atctggacgg tcatgcgctg gcctttgacg ccttcgagcg gcaggatgag ggcggcgctt
     9961 tctccaggct cgagctccag ctcgcgcaat tgggcgaggc gtccggcgag ggtgacctca
    10021 agcgtggagg tgacgcggcg cgcgttggcg ggggcggcgg tgactttgac aaaggcctcg
    10081 tagcggtcat gcgcgagcgg ggcggggcgg atctgaaagg cggtgatgcc ggcgttgagc
    10141 ggggcgtcca aggcggcgct gtggaattga taggcgagtt ccgcgccggc ttcgaaagcg
    10201 cggtcgccga aatgccagac ctgggtgccg ggctccaact cggcgaggcg cgcggcggtg
    10261 atgagggcga tgtccggccg cagggtgagc gggcgcgggg cgagttcatc gagcaggcgc
    10321 aggcactcgc ggcggttgcg gctgcgggag agcaggacat gcgccttggc atcgcaggcg
    10381 acgagggaga cgacgacttg ctccggcagg gatttgagtt tggcgcgggc gagctggcgc
    10441 gcctcctcca gccgggtgcg gcctttttca tcgcgcgcgg ccatggaggc ggagcggtcc
    10501 atcaaaagca cgactgcgcc gggcgagtcc gcgccggcct cgcccacggg ccgggcgagt
    10561 gccaggacga tgaggaaaag catgagcagg gtgagcagca gggagagcca cttttttatc
    10621 ttgcgcagcc aggcggactc ctggtgctcg cgggcgagcg agcggaagaa gagcagcgtg
    10681 gagacgggca cgcggcgggc ttttttcttg aagaggtaaa gcgccagcgg gatgagcgcg
    10741 agccaggcca gatgcaggag atggggtgcc aggaaacgca tcgcgggagc agggtgtcac
    10801 acgccgccgg cgagggcgga gccgcggttc aggaggtcga ggaagcggtc ctcaaagggc
    10861 tcggtggcgc cgcattgcat gaagtccatg cggtgcgcgg cgcactcgcg ggcgagggtg
    10921 tcgcaaaagg cgtcgaaggt ctcggagtag cgctgcacat cccggcgggt gagccagaag
    10981 cggcggcgct cgccggtctc cacctcgtcg agctccacct cgccctcgat ccgcggctcg
    11041 cgctcctcgg ggtggaggat ctgcacaaaa tgcgtctcgc ccggccaggc ggcggcgcgc
    11101 tggatggcat ggctggcggt gttgacatcg cgcccgaaga aatccgagag cacaaagatg
    11161 actccatagc gctggcgggt ggggcggaac tcggtgaagc cgcgctccag atcggtgaga
    11221 ccgccgaagt cgaggcgcgt ggcggcctgg atgatttttt cgatgttgcc ctgcccgcgc
    11281 aggggcggca gctcctggcg cacggtgtcc gagagtgaat aaaggctcac cctttgctgc
    11341 gcggccaggc cgagataggc gagggcgacg gcgaagcgca gtgcctgtcg gcgcttttcc
    11401 ccgaaggggc gcgccatgga ggcgctggtg tccaccagca catgcaggtt cagctcctgc
    11461 gtctcctcgt agagcctgac aaaaagcttg tcgagccgcg cgtagagccg ccagtcaatg
    11521 gcgcggtaat cctcgcgcgg cgtgtagtgg cggtagtcct tgaactcgcg tgtgaagccg
    11581 gtggccgggg tggactgcgc gccgcggcgg gagagcgcgc ggcgtttgcg caggcgcacg
    11641 gccagggagc gcagccggtc gagaaaggcg gcgtcgaaga ggtcctcgcg ctcgagggcg
    11701 gcctggctca tgcctgcggg gcctcctcat ctgcgacatc ggcggtctca tccagcggtt
    11761 gatcggggtc gagcgcggtg agggcgtaga tgccgctgag gggaatctcc acgcgcagtt
    11821 cctccagcct catcaccgcc tgccagccgt cgaaggaagt cagacgccag ctatcatccg
    11881 cctgccgctc atacacctcc accagcgcct gggcggtgga gacgagcaca tagcagcgca
    11941 gttcgggcag atggcggtag tgccagaatt tttccccgcg gtcgtaggcc tcggtggccg
    12001 ggctgagcgc ctcggcgatg aggccggggt tgagcaatgt gaggccgttg gcggtgaagc
    12061 gcggtttgtg gcagacgatg gagagatccg ggaatgtgtt cagccccgtg gcctcgactt
    12121 tgaccttcat gtcgctgtca aagacccggc agccgcgccc ggcgagggcg cccttgaagc
    12181 aatggccgag attggacttc accagactgt gggaggggct gcctcccgcc atggcgatga
    12241 tcctgccgtc gcggaactcc agcttgtcct ccgacttcgc caggatggcc tcatactccg
    12301 cctcggtgat cctgggcggc ttcggcgggg tatggacgct gggtgcgttg agcttgatgg
    12361 cgacggcggg catggcggca gaatacacgg tcccggacgg aggggctatc aaaaggatgg
    12421 ggctgacttc tccgaccctg tgagcagcag ctcacgccgc ctcctgcgcc ggggttttga
    12481 tttggtcgag cagcttggtg atgatgtcgt ccactttgac ctgctgggcc tcgccttcga
    12541 agttcaagat cacgcggtgg cgcagggcca catgggcgag ggcgcgcacg tcctccgtgg
    12601 agacggcggt ggcgccatgc acggcggccc agatggcgag gatccacacg atggcggga
//
LOCUS       DAOMYJ010000153        12636 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Prosthecobacter sp. isolate AMR_MDS_5629
            NODE_1666_length_12636_cov_20.883237, whole genome shotgun
            sequence.
ACCESSION   DAOMYJ010000153 DAOMYJ010000000
VERSION     DAOMYJ010000153.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867850
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prosthecobacter sp. (wastewater metagenome)
  ORGANISM  Prosthecobacter sp.
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Verrucomicrobiaceae; Prosthecobacter.
REFERENCE   1  (bases 1 to 12636)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12636)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.30
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:34:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,742
            CDSs (total)                      :: 4,684
            Genes (coding)                    :: 4,676
            CDSs (with protein)               :: 4,676
            Genes (RNA)                       :: 58
            tRNAs                             :: 55
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12636
                     /organism="Prosthecobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1666_length_12636_cov_20.883237"
                     /isolate="AMR_MDS_5629"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1965333"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..218)
                     /locus_tag="PLP58_15000"
     CDS             complement(<1..218)
                     /locus_tag="PLP58_15000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase, biotin carboxyl carrier
                     protein"
                     /protein_id="HRJ09883.1"
                     /translation="MDEPKPTEKEATALDLKQIKQIVALMAENDLSYFHFENQGSKLE
                     LRRGQDLETFQKLLKHLPVSAPAPAAAYP"
     gene            complement(309..1010)
                     /locus_tag="PLP58_15005"
     CDS             complement(309..1010)
                     /locus_tag="PLP58_15005"
                     /inference="COORDINATES: protein motif:HMM:NF024778.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HRJ09884.1"
                     /translation="MTSIDTSAAAFLAGLVTSIHCVGMCGPLSCSWAVSSKPGSVSFF
                     RDTGLYHGGRLVSYALVGALAGTAGVVPLGFFQHGAGIVLPWLMVLAFALVGMGLDSR
                     LPKPGFLARPLRRLQSAAFKMKSGPRSGLLGLATPLLPCGPLYMIFALALANGSALKG
                     AGFTLAFGLGTLPLLWLAQTGLQRLGGTLEPATLRRLQRGLALVTAVILAWRLRGTLD
                     FGSDTVPSCCHQAGL"
     gene            complement(1007..1180)
                     /locus_tag="PLP58_15010"
     CDS             complement(1007..1180)
                     /locus_tag="PLP58_15010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ09885.1"
                     /translation="MPLSIRKILSNKPWLLVVLLLAAFLAAWFCFIQIALKNQPSQVP
                     LEPLPAHNAAEVR"
     gene            complement(1146..2567)
                     /gene="ccoG"
                     /locus_tag="PLP58_15015"
     CDS             complement(1146..2567)
                     /gene="ccoG"
                     /locus_tag="PLP58_15015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009958925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008535 - respiratory chain complex IV
                     assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase accessory protein CcoG"
                     /protein_id="HRJ09886.1"
                     /translation="MNQPTLTTLSTINADGSRKFVHPADVRGRFTFWRKVSALVLLVI
                     YAALPWIPVGGFPAVFLDVQTRRFHFFGLTLAAQDLWVGFFLITGLAFALFFVTSLFG
                     RVWCGWTCPYTVFLEHVYRRIERWVDGDGPARRRLEKAPWTAGKITRRAVKHALFILC
                     SALIAHIFLSYFVSIHGLYGMMQSSPQEHRLAFGVVVFLTGALYFSFSWFREQFCIIL
                     CPYGRLQSALTDDNSIVIGYDKKRGEPRGKAGAAAGDCVDCRRCVQVCPTGIDIRNGL
                     QLECIGCAACVDACDEIMTRLQRPAGLVRYDSHTGLQGGRTRLLRARTVFYSCILLAG
                     TAAFLFATTRIEPLRASVVRMVGAPYYAGEGVVRNQFLVRVINKRNAPSSYQLEVTGR
                     TPQELQVTGLQSALELPAMGEEQKAIVLSLPEAAYNGPFKLQIKVTESGTGASMTTRV
                     MEFLGPDPRLKSNAPLNPKDFIQ"
     gene            complement(2661..4142)
                     /locus_tag="PLP58_15020"
     CDS             complement(2661..4142)
                     /locus_tag="PLP58_15020"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HRJ09887.1"
                     /translation="MFPRLAPAFLLTGLLAASAAKPPEPTRQPLHRSVDLSLGESAVV
                     TLSDGTKATVRLLDLEERVDSMSAAVREARVRIEVNGETLWISSANYHLPAQAGGAQV
                     DCPITRGYNANSGQDSWGLKKDARLRLWPAGSPWIEPELFALPCRQRWFATSTQFSNE
                     PTYVDGGDRPDRKQIYYHNDLDFGGCEALVEVVAATDGLVVSVGESVLPGYIGTPVRP
                     RYDVVYVLDDRGWYYRYSHLHSIEESVKPGTRLKLGQKIGLLGKEGASGGWSHLHFGI
                     KSRQPSGEWGTEEAYAFAWQAALREQKPAAIAVARPHHFVRVGERLVLDGSKSWAAAG
                     IEKYEWALTDGTSAAGPRVERGYDQPGCYSEVLKITGRDGAMSYDFAIVQVIGDTDPK
                     LIPPAIHATCAPSLGVKAGDEITFKVRVFRSTGGETWDFGDGTPPVQTRSDGNAVKLA
                     RDGYAATTHRFEKPGDHLVRVEHVNERGEKAVAHLWVPVAAAR"
     gene            complement(4323..5246)
                     /locus_tag="PLP58_15025"
     CDS             complement(4323..5246)
                     /locus_tag="PLP58_15025"
                     /EC_number="4.1.3.3"
                     /EC_number="4.2.1.41"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009961832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009436 - glyoxylate catabolic process
                     [Evidence IEA];
                     GO_process: GO:0071704 - organic substance metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HRJ09888.1"
                     /translation="MKTTPVTAADLRSSVIAVPPLCRGADLSLSREQNARLIQHMHAG
                     GIRTLLYGGNANLYNMAPSEYPALLTMLRELAPEDLWMVPSVGPMYGTAMDQAAVLRD
                     HEFPTAMLLPTLFPSKPEGVATAVRHFVEKSGIKAVLYMKDEAYITPELAAALVNDGV
                     ISWIKYAVVKEDPAVDPYLSKLVSLVNPEMIVSGIGEQPAIVHLRDFGITGFTAGCVC
                     VAPRPSMALLAAIQAGDFEKAETLRQIFRPLEDLRNAHSPILVLHHAVALAGVADTGP
                     VLPLLTGLPDALLPGIEQAARELLARNVNPV"
     gene            complement(5311..6075)
                     /locus_tag="PLP58_15030"
     CDS             complement(5311..6075)
                     /locus_tag="PLP58_15030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ09889.1"
                     /translation="MKTSLAYILVLLGAAALAWRASAPEQPGLENSRDHPSTKTLVFV
                     PKGREAVTNEPDNEAVTQCDAWITLGTDAAIQQWSDAVHAEADPVLRLQMTAALDALS
                     SREGLELVTSALVFATDETVLDAVQRTITRMADADTVAHLAELRVRADLGESQRAHVL
                     AALAGIHNTEALEAQARLAATHPAAEVRAAAAASLALMDTPESALALIHARAALPPEA
                     GIERSMLLHHLSRMSAAPPVLETAEDPALRGLLAAR"
     gene            complement(6126..8555)
                     /locus_tag="PLP58_15035"
     CDS             complement(6126..8555)
                     /locus_tag="PLP58_15035"
                     /inference="COORDINATES: protein motif:HMM:NF012311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HRJ09890.1"
                     /translation="MKITSLLALLLALGSIQSHAQEFVTFNAGKRIVLKPDPAQTHIR
                     LAQSGAIVADAVKQELQQPRPGGFARLKRPVVAGLKPLDEAVQSRAAVSALLRNSQVA
                     FAAPVLRSAEGHELSPTSSLLVKLKPGQTMAKFLQVLRNPAIVNVRQISRDGVHKLTT
                     NLRDGAAVMDLAATLVKHPMVKMAQADFSHEAVSMLVPNDPFFFSSAAWGFSNSGNLG
                     GLAGFDMNAVKAWDVTTGSSAIIVAIFDGGIQQDHPEINQIPGRDFTDDPITNGGTPA
                     GSVAGHDHGTMVASCVSARINNARSACGIAPDVRCVSVRWGVPDGEGHFIGNDSNVIE
                     GLEWAQSIGARVSVHSYGMFPSWALDEVFESTREAGMIHFAATGNDAHKIFLFEQFPA
                     IAWPASSPYVLAVGAARRDGVRAPLSRYGGDTRRVAQSVDFMAPGEEVAVVARTGTPG
                     VADTDALPGQGTSFAAPYAAGVAALLLSQNPALTPAQVEARMIAGCQNMGTRGWDRFT
                     GYGLVDAFRSMADDHGDTPATSTRVALNSVTRGHLNREDDADCIRFTVPATLSIVRMH
                     MNALFRGDILTPSAATTVEVLNENGTRATFTEIPVAPRDSSVVFPGAGLSGFKLRAAF
                     TPGSYVLRVRGLNSAMGSYELVLSEEDGQPEISVLGNNSVIADGDTTPATADGTQFGT
                     VRAGTSVTRTFTIRNDGQNILRVTNANNIDGNRVLTPVIQPGSAANGVRDIPVPEPKP
                     VPAPIHFRVTSMPVAQILPGRSATFIVTFKPVGTGFFTHTLRIQSNDQDEREYDFVIS
                     GGSLLQPSSVR"
     gene            complement(8700..9488)
                     /locus_tag="PLP58_15040"
     CDS             complement(8700..9488)
                     /locus_tag="PLP58_15040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009963108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NIPSNAP family protein"
                     /protein_id="HRJ09891.1"
                     /translation="MKRSAFLLLPAFAATLGLAATAQAADERVYEMRVYVTNEGKLDA
                     LLARFRDHTCKLFEKHGMENIGYWVPLAKEDGADNTLIYILAHKSRDAAKASFAAFGK
                     DPEWQAARKASEEGGKILAKAPESIFMSVTDYSPPVKTGISGKNGVFELRTYTTVPGK
                     LEALHARFRDHTMALFTKHGMTHTGYWTPVDEDKGAGVKLIYILGHASQEAGLASFTA
                     FRADPVWIAAKSASESANGGSLTIPQPDGVRSVYLRAVDFSPIQ"
     gene            9591..10649
                     /locus_tag="PLP58_15045"
     CDS             9591..10649
                     /locus_tag="PLP58_15045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ09892.1"
                     /translation="MKTHLLITAFLAAAAPAPGQGGAGEPAGQWKAPANDGRSWKHGG
                     TGLAFPQLLGTSRLAGEFEYKDGGTFIRYENLDERSRADIFLFKTGAPLPALEDRHRR
                     ILSEMDNVVSDMEAMVRQGRYKNLDAGALQGGELELWQQQSLPIATRQFTATRLGISK
                     EGSAEAVIKIWVGITIVADHILTIRHMRPADTGEAGEQSMKTFAGLVFQIIKDPSLRG
                     HIRELLAAYLKNPLSDDGEQAAAAVLAYLEQAPWFPINIPEDPVSGWMEKFEKSAPGT
                     QDHLLRAFMLGAAKAAFEDADALACLNAGARQFAVIYRELARQHPQITLPEVDSFAAA
                     AEKDLGAAWLKDHAHPAR"
     gene            10662..11768
                     /locus_tag="PLP58_15050"
     CDS             10662..11768
                     /locus_tag="PLP58_15050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009960953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ09893.1"
                     /translation="MKALSFLPLLAAASAHAAPLDTPLAAIRAVGREGAGNENASAAW
                     RQVSQADAAALPEILKGMNGANPLAENWLRAAVGAVADKAIAAKTLPVPALQAFLMDT
                     ANSPGPRVLAFDLIQRADPALAGSITASLLDDPSNELRRHPVASLIQAGDEALAQGKK
                     DAATAAYQQAMRGARDEDQIKDLAKKLKDLGQPVDLPRHFGFIMHWKLIAPFTNAERK
                     GFDTVFPPEKEIVLDAEYEGKGGDKARWQDFTSQDDYGMVDFNKPFTMLKEVTGYAFT
                     EFHSAEERDAQIRLGCKNGWKVWFNGELLFARDEYHRGAKLDQYKLPVRLRKGKNTIL
                     VKCCQNEQKETWTVEWQFQLRVCDATGTAIHPVP"
     gene            11784..12164
                     /gene="crcB"
                     /locus_tag="PLP58_15055"
     CDS             11784..12164
                     /gene="crcB"
                     /locus_tag="PLP58_15055"
                     /inference="COORDINATES: protein motif:HMM:TIGR00494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:1903425 - fluoride transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:1903424 - fluoride transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fluoride efflux transporter CrcB"
                     /protein_id="HRJ09894.1"
                     /translation="MRNTLLIFLGGGLGSVLRFWTVLAAQRCWPGAVFPAGVLVANLL
                     GSFILGFLFAWPAMRARDAGAWFFCATGVLGGYTTFSTLAGDSLQLLLNNHPLPALLN
                     AFGSITAGLLAAALGWQTARFIFG"
     gene            complement(12169..>12636)
                     /locus_tag="PLP58_15060"
     CDS             complement(12169..>12636)
                     /locus_tag="PLP58_15060"
                     /inference="COORDINATES: protein motif:HMM:NF013847.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ09895.1"
                     /translation="EANVPGNPADAPLAARGINRVLDAARGEKAVHFCFGNYGGQTIQ
                     KGGWAALVDFLNSLRADHLVLELAHRPEADLDALRELEARVGVGIGVVDIKVNHIESA
                     EEIARRIERAERRLGAGRVKYVHPDCGFWMLKRSIADRKIEALARGRDLYLGR"
BASE COUNT         2209 a         4185 c         4148 g         2094 t
ORIGIN      
        1 gggtaggctg cggcgggagc cggagcggac actggcaggt gcttgagcaa tttctgaaaa
       61 gtctccaagt cctgcccgcg gcgcagctcc agtttggatc cctggttttc aaagtgaaag
      121 tagctgagat cattctcagc catcagcgcc acgatctgtt tgatctgttt caagtccaag
      181 gcggtggcct ccttttcggt tggtttgggt tcgtccattc ggggaatgtg agtgcgttga
      241 gcgcgtgaga ctaaccgctg tcccgttgga ggcaaggagt aaaggaaggc gcagtccggc
      301 cctcattctc acaatccggc ctgatgacag cacgagggta cggtgtcact gccgaagtcg
      361 agcgtcccgc gcaagcgcca ggcgaggatg acggcggtca ccagggccag gccacgctgg
      421 agccggcgca gtgtggccgg ttccaacgtg ccgccgagcc tttgcagccc cgtctgcgcc
      481 agccagagca ggggcagggt gccgaggccg aaagcgagtg tgaagcccgc gcccttgagc
      541 gcggagccgt ttgccagcgc gagagcgaag atcatgtaaa gcggaccgca tggcagcagc
      601 ggcgtggcca ggccgagcag gccggagcgc gggccgctct tcattttgaa ggccgcgctt
      661 tgcagccggc gcagaggccg ggccagaaaa cccggcttgg gcaggcgcga gtccagcccc
      721 attccgacca gggcaaaggc cagcaccatg agccacggca gcacgatgcc cgcgccatgc
      781 tggaagaacc ccagaggcac cacgcccgcc gtcccggcaa gcgcccccac cagcgcgtac
      841 gaaacaagcc tgcctccatg ataaagcccc gtgtcgcgga aaaaagacac cgacccgggc
      901 ttggaactca ccgcccacga gcacgacagc ggcccgcaca tgcccacgca atgaatgctg
      961 gtgacgagtc cggcgaggaa ggcggcggcg ctggtgtcta tcgaggtcat cggacttcgg
     1021 cggcgttgtg cgcgggcagg ggttccagcg gcacctgtga cggctggttt ttcagggcga
     1081 tctggatgaa gcaaaaccac gcggccagga atgcggccag cagcagcacc accagcagcc
     1141 agggcttatt ggataaaatc tttcggattg agaggggcat tggatttgag gcgcgggtcg
     1201 ggaccgagga actccatcac gcgcgtggtc atgctggcgc cggtgccgga ttctgtgacc
     1261 ttgatctgga gtttgaaggg gccgttgtag gcggcctcgg gcagggaaag cacgatggct
     1321 ttttgctcct cgcccatcgc gggcagctcc agcgccgact ggagtccggt cacctgcagt
     1381 tcctgcggtg tccggccggt gacttcgagc tgataggatg agggcgcgtt gcgcttgttg
     1441 atgacgcgca cgaggaactg gttccgcacc acgccctccc ccgcgtaata cggcgcgccc
     1501 accatgcgca ccacgctggc gcgcagcggc tcgatgcgcg tggtggcgaa gagaaatgcc
     1561 gccgtgcccg ccaggaggat gcacgaataa aagacggtgc gcgcccgcag cagccgcgtc
     1621 ctgcctccct gcaggccggt gtgcgagtca tagcgcacga ggcccgccgg gcgttgcagc
     1681 cgggtcatga tctcgtcgca ggcgtccacg caggccgcgc agccgatgca ctccagttgc
     1741 agcccgttgc ggatgtctat gcccgtgggg cagacctgca cgcagcgccg gcagtccacg
     1801 cagtcacccg ccgccgcgcc cgccttgccg cgcggctctc cgcgcttttt gtcatagccg
     1861 atgacgatcg agttgtcgtc cgtcagcgct gattgcagcc ggccatacgg gcacaggatg
     1921 atgcagaact gctcgcggaa ccaactgaac gaaaaataca gcgcgcccgt caggaagacc
     1981 accacgccaa aggccaggcg gtgctcctgc ggcgagcttt gcatcatgcc gtagagaccg
     2041 tggatggaga cgaagtagct cagaaaaatg tgcgcgatga gcgcggagca gagaatgaac
     2101 aaggcgtgct tcaccgcgcg gcgggtgatt ttccccgccg tccacggcgc cttctccagg
     2161 cggcggcggg cgggtccgtc gccatccacc cagcgctcga tgcggcggta cacatgctcc
     2221 aaaaagacgg tgtaggggca ggtccacccg caccagacgc ggccgaacag ggaggtgacg
     2281 aagaacaggg caaaggccag cccggtgatc aaaaagaagc ccacccacag gtcctgcgcc
     2341 gccagtgtca gcccgaagaa atgaaaacgc cgcgtctgca catcgaggaa gaccgccggg
     2401 aagccgccga ccggaatcca gggcagggct gcgtaaatga ccagcaacac gagcgcggag
     2461 accttccgcc aaaaggtgaa ccgcccgcga acatccgccg gatgcacaaa cttccgcgaa
     2521 ccatcggcat tgatggtcga gagcgtggtg agtgtcggct ggttcatggg tcggagcggg
     2581 ggaaaactcc tgcgctaccc accatgcgcg gagcacaggg caaatctccg cccgctttgc
     2641 gatagctggc accgatcttg tcagcgggcc gccgccaccg gcacccacag atgcgccacg
     2701 gccttctcac cgcgctcatt gacatgctcc acgcgcacga ggtggtcgcc cggcttttcg
     2761 aagcggtgcg tggtggcggc gtagccatcc ctggccagct tcaccgcgtt gccatcggag
     2821 cgtgtctgca caggcggagt gccgtcgccg aagtcccagg tctcgccccc cgtggagcgg
     2881 aacacgcgca ctttgaaggt gatctcgtcg ccagccttca cgcccaggga gggcgcgcaa
     2941 gtcgcgtgga tcgcgggcgg gatgagcttc gggtccgtgt cgccgatgac ctggacgatg
     3001 gcgaagtcat aactcatcgc gccatccctg ccggtgatct tcaaaacctc gctgtaacag
     3061 ccgggctggt catagccgcg ctccacacgc ggcccggcgg cggaggtgcc gtcggtcaaa
     3121 gcccactcat acttttcaat gcccgccgcc gcccaggact tcgagccatc cagcaccagg
     3181 cgctccccca cccgcacgaa atgatgcggg cgcgccacgg cgatggcggc gggcttttgc
     3241 tcccgcaaag ccgcctgcca ggcgaaggcg taggcctcct ccgtgcccca ctcgccggaa
     3301 ggctggcggc tcttgatgcc gaaatgaaga tgcgaccacc cgccgctggc gccctccttg
     3361 cccagcagcc cgatcttttg ccccagcttc aagcgcgtgc ccggcttcac actttcctca
     3421 atgctgtgca ggtggctgta acggtaatac cagccccggt catccagcac atagaccaca
     3481 tcgtaacgcg gccgcaccgg cgtgccgatg tagcccggca gcacgctctc ccccacgctg
     3541 acaacgagtc catccgtggc cgccaccacc tccaccagcg cctcgcagcc gccgaagtcg
     3601 aggtcgttgt ggtaatagat ctgtttgcgg tccgggcggt cgccgccgtc cacataggtg
     3661 ggctcgttgc tgaactgcgt gctggtggcg aaccagcgct ggcggcaggg cagcgcgaac
     3721 agctccggct cgatccaggg cgagcccgcc ggccagagcc gcagccgcgc gtcctttttc
     3781 aagccccagg aatcctggcc cgagttcgcg ttgtagccgc gcgtgatggg gcagtccacc
     3841 tgcgcgccgc cggcctgcgc gggcagatga tagttggcgc tgctgatcca aagcgtctcg
     3901 ccgttcacct cgatcctcac cctcgcctcg cgcaccgccg cgctcatcga gtccacccgc
     3961 tcctccagat cgagcagcct cacagttgcc ttcgtgccgt cagaaagcgt caccactgcg
     4021 gactccccga gcgaaagatc caccgaacga tgcagcggct gccgtgtcgg ctccggcggt
     4081 ttggcggcgg atgcggccag caggccggtc aggagaaaag cgggggcaag gcgtggaaac
     4141 atccaccttc tacgcccgcc agcgggggct tttcacgagt cggcgcgccg cgtggccaag
     4201 gcgacttctc atgcgcccgc ctcaagacgg gccggcgcag ttggatcccc gcttcttgaa
     4261 gtgatggatt ttcaatgccc cggcactcca cgactccagc cgcgggccgc cacccttggt
     4321 gatcacacgg ggttgacgtt ccgcgccagc agctccctcg ccgcctgctc gatgccgggc
     4381 agcagcgcgt ccggcaggcc ggtcagcagc ggcagcaccg gtccggtgtc cgccactccc
     4441 gccaaagcca cggcgtggtg cagcaccaaa atggggctgt gcgcgttgcg cagatcctcc
     4501 agcgggcgga agatctgccg cagcgtctcg gccttctcaa agtcacccgc ctggatggcc
     4561 gcgagcagcg ccatgctcgg gcgcggcgcc acgcacacgc agcccgccgt gaagcccgtg
     4621 atgccgaagt cccgcagatg cacgatggcc ggctgctcgc caatgcccga gacgatcatc
     4681 tccgggttca ccagcgatac cagttttgac aggtaggggt ccaccgccgg gtcctccttc
     4741 accaccgcgt acttgatcca ggaaatcacc ccgtcattca ccagcgccgc cgccagctcc
     4801 ggcgtgatgt aggcctcgtc cttcatgtac agcaccgcct tgatgccgga cttctccaca
     4861 aaatgccgca ccgccgtggc caccccctcc ggcttcgagg ggaaaagcgt cggcagcagc
     4921 atggccgtgg gaaactcatg gtcccgcagc accgccgcct gatccatcgc cgtgccgtac
     4981 atcgggccca cgctcggcac catccacagg tcctccggcg ccagctcccg cagcatcgtc
     5041 agcagcgccg gatactccga gggcgccatg ttgtacagat tcgcattgcc cccgtaaagc
     5101 agcgtgcgga tgccgcccgc gtgcatgtgc tggatgagcc gcgcgttttg ctcccggctc
     5161 agggaaaggt ccgccccgcg gcaaagcggc gggacggcga tgacggaact gcggagatcg
     5221 gcggcggtga cgggcgtggt cttcatgcgg cccgcacagt gggagggcgc gcgccttggg
     5281 caaagggaaa atcactctgt gaaagaagtc tcaacgcgcg gccaaaagcc cgcgcagggc
     5341 ggggtcctcg gcggtctcca gcacgggcgg ggcggcgctc atgcggctga ggtgatgcag
     5401 gagcatggag cgctcgatgc cggcctcggg cggaagggcg gcgcgcgcgt ggatgagggc
     5461 gagcgccgac tcgggggtgt ccatgagcgc gaggctcgcg gcggcggcgg cacgaacctc
     5521 ggctgccggg tgcgtggcgg caaggcgggc ctgcgcctcg agggcctcgg tgttgtgaat
     5581 gccggcaagc gcggcgagga cgtgggcgcg ctggctttca ccgagatctg cccggacgcg
     5641 cagctcggcg aggtgggcca cggtgtccgc gtccgccatg cgggtgatgg tgcgctggac
     5701 ggcgtcgagc acggtttcgt cggtggcgaa gacgagggcg gaggtgacga gttccaggcc
     5761 ctcccggctg ctgagcgcgt cgagcgcggc ggtcatttgc agacggagga cggggtcggc
     5821 ctcggcatgg acggcgtccg accactgctg gatggcggcg tcggtgccga gggtgatcca
     5881 cgcgtcacat tgagtgaccg cttcgttatc aggttcatta gtgaccgcct cccggccttt
     5941 tggaacaaag acgagggttt tggtggaagg atggtcgcgg ctattctcca gtcctggttg
     6001 ctccggagct gatgcccgcc atgcgagcgc tgcggctccg agcaagacga ggatgtaggc
     6061 gagtgatgtt ttcatggcgg gcatcagggt tgacggtttg ttcagcggtt gggattgtct
     6121 cgttgttagc ggacggagct tggctgcaag agggagccgc cgctgatgac gaagtcgtac
     6181 tcgcgctcat cctggtcatt cgattgaatc ctgagcgtgt gggtgaagaa gcctgtgccc
     6241 accggcttga aggtcacaat aaaagtcgcg ctacggcccg gcaggatttg cgccacaggc
     6301 atgctggtca cacggaagtg gatcggcgca ggcacgggct tgggttccgg gacgggaata
     6361 tcgcgaacac cattggcagc gctgccgggt tggatcaccg gagtgaggac gcggtttcca
     6421 tctatgttgt tggcattggt gacgcgcagg atgttctggc cgtcgttgcg aatggtgaag
     6481 gtgcgggtga cggaggtgcc ggcacgcacg gtgccaaact gcgtgccgtc cgctgtggca
     6541 ggtgtggtgt cgccatccgc aatgactgag ttgttgccca gcacggagat ctccggctgg
     6601 ccgtcttcct cggagagcac cagttcataa ctgcccatgg cgctgtttag cccgcgaacg
     6661 cgcagcacat aactgcccgg tgtgaaagcg gcgcggagct tgaagccact gaggcccgca
     6721 ccgggaaaca caacactcga atccctgggg gccacgggga tttccgtgaa ggtggcgcgt
     6781 gtgccgtttt cattgaggac ttcaactgtc gtggctgcgg agggggtcag aatgtcgcca
     6841 cgaaacagag cgttcatgtg catcctcacg atgctcagcg tcgccggcac ggtaaagcgg
     6901 atgcagtccg cgtcatcctc gcggttgaga tgaccgcgtg tcaccgagtt cagcgcgacc
     6961 cgtgtggagg tggcaggcgt gtcgccatgg tcatcagcca tgctgcggaa ggcgtccacg
     7021 agtccatagc cggtgaagcg gtcccagccg cgagttccca tgttctggca gccggcgatc
     7081 atccgcgcct cgacctgcgc gggggtgagc gcgggatttt gggaaaggag cagagctgcg
     7141 acaccagcgg cataaggagc ggcaaaagag gtgccctgac ctggcaaagc atccgtgtca
     7201 gcgactcccg gcgttccagt gcgggcgacc acagcgactt cctccccagg agccatgaag
     7261 tccacgctct gtgccacacg acgggtatcg ccgccgtatc tcgagagggg cgcacgcacc
     7321 ccgtcacgcc gtgctgctcc aacggccagc acataggggc tgctggccgg ccaggcgatg
     7381 gcggggaact gctcgaagag gaagattttg tgagcgtcat tgcctgtggc cgcaaaatgg
     7441 atcatgcctg cctcacgggt ggattcaaaa acctcatcca gcgcccagct tggaaacatg
     7501 ccgtaggaat gcacgctcac acgcgcaccg atggactgtg cccattccag tccctcgatc
     7561 acattgctgt cgttgcctat aaaatggcct tcgccgtctg gcacgcccca gcgcaccgag
     7621 acacagcgca cgtccggcgc gatgccgcag gcgctgcgcg cattgttgat ccgggcggac
     7681 acacagctcg ccaccatggt gccgtggtca tgcccagcca cacttccggc gggcgtgccg
     7741 ccatttgtga tgggatcatc cgtgaagtcg cggcctggga tctggttgat ctccgggtgg
     7801 tcctgttgga tgccaccgtc aaagatcgcc acgatgatcg cagacgagcc ggtggtcaca
     7861 tcccaggcct tgaccgcatt catgtcaaag ccggcaagcc cccctaagtt tccactgttg
     7921 ctgaaacccc atgcagctga tgaaaaaaag aaaggatcgt tgggcaccag catcgagacg
     7981 gcctcatgcg agaaatccgc ctgcgccatc ttcaccatcg ggtgctttac cagtgttgcg
     8041 gccaggtcca tcacggcggc gccgtcacgc agattcgtgg tcagcttgtg gacgccatcc
     8101 cggctgatct ggcggacgtt gacgatggcc gggttgcgca gtacttggag aaactttgcc
     8161 atcgtctggc cgggcttgag cttcaccagc agggagctgg tgggtgaaag ctcgtggcct
     8221 tcggcggagc gcagcactgg cgcggcaaag gcgacctggc tgtttctgag cagtgcggac
     8281 acggcggcgc gggattgcac ggcctcgtca agaggcttca gaccggcgac caccggacgc
     8341 ttcaaccgcg caaaaccgcc tgggcgcggc tgctgcagct cttgtttgac ggcatctgcc
     8401 acgatggcgc cgctttgggc aagccggatg tgggtctgcg cggggtccgg cttgagcacg
     8461 atgcgcttgc cggcattgaa ggtgacgaac tcctgcgcgt ggctttgaat ggagccaagc
     8521 gcgagcagga gggccaggag ggatgtgatt ttcatgatgg gtggagtgtg agtttccggg
     8581 gctgatgagc gccccatgcc atcccgatgc gcggccccgg cgggacttgc gcaattcatg
     8641 aaaaaaagag cgcgcccctg gttgccgggg gcgcgcttat cacaaaacag cggcgggctt
     8701 tactgaatcg gtgagaaatc caccgcgcgc aggtacacgc tcctcacgcc gtcgggctgg
     8761 gggatggtga gggagccgcc gttggcggac tcggaggcgc ttttggcggc gatccagacg
     8821 gggtcggcac ggaaggcggt gaaggaggcg aggccggcct cctggctggc gtggccgagg
     8881 atgtagatga gcttcacgcc cgcgcctttg tcctcatcca cgggagtcca atagccggtg
     8941 tgggtcatgc cgtgcttggt gaagagggcc atggtgtggt cgcggaagcg ggcgtgcagg
     9001 gcttcgagct tgccgggcac ggtggtgtag gtgcgcagtt cgaagacgcc gtttttgccg
     9061 ctaataccgg ttttcacggg cggagagtag tcggtgacgc tcatgaagat ggactcgggg
     9121 gctttggcga ggattttgcc gccctcctca ctggctttgc gcgcggcctg ccactcggga
     9181 tctttgccga aagcggcgaa ggaggccttg gcggcgtcgc ggctcttgtg ggcgaggatg
     9241 tagatgaggg tgttatcggc gccgtcctcc ttcgccagag gcacccagta gccgatgttc
     9301 tccatgccgt gcttttcaaa gagcttgcag gtgtggtcgc ggaagcgggc cagcagcgcg
     9361 tcgagcttgc cctcgttcgt cacatagacg cgcatctcat acacgcgctc atcggcggct
     9421 tgagcggtgg cggcgagacc gagtgtggcg gcgaatgcgg gcaggaggag aaaggcggag
     9481 cgtttcatgg tggcagtgaa acacgaagcg gcgcgcggct ttgttgcgcc gaatcgccgg
     9541 cagcccagct ttgccgttgc ggcgggttgg acggctggca tcgtgcgcgc gtgaaaaccc
     9601 acctgctcat caccgctttt ctggccgccg ccgcccccgc cccgggtcaa ggcggcgcag
     9661 gcgagcccgc cggccaatgg aaggctccgg caaacgacgg acgctcctgg aagcatgggg
     9721 gcaccggcct ggccttcccg cagttgctcg gcacaagccg cctggcgggc gagttcgagt
     9781 acaaggacgg cggcactttc atccgctacg agaacctcga cgagcgctcc cgcgccgaca
     9841 tcttcctttt caaaaccgga gcgccattgc ccgcgcttga agaccgccac cgccgcattc
     9901 tctccgagat ggacaacgtc gtctcggaca tggaggccat ggtgcggcag gggcgctaca
     9961 aaaacctcga cgccggcgcg ctccagggcg gcgagctgga gctctggcag cagcagtccc
    10021 tgcccatcgc cacccggcag ttcacagcca cgcgcctcgg catctccaag gagggctccg
    10081 ccgaggccgt catcaaaatc tgggtcggca tcaccatcgt cgccgaccac atcctcacca
    10141 tccgccacat gcgccccgct gacacgggtg aggccgggga gcagtccatg aagacttttg
    10201 cgggccttgt tttccagatc atcaaagacc cctccctgcg cggccacatc cgggagttgc
    10261 tcgccgccta cctgaaaaac cccctctccg atgacggcga gcaggccgcc gccgccgtgc
    10321 tcgcctatct cgagcaggcg ccctggtttc ccatcaacat cccggaggac cccgtcagcg
    10381 gctggatgga gaaattcgaa aaaagcgcgc ccggcaccca ggaccacctc ctgcgcgcct
    10441 tcatgctcgg cgcggcgaag gccgccttcg aggacgcgga cgccctggcc tgcctgaacg
    10501 ccggcgcgcg ccagttcgcc gtcatttacc gcgagctggc ccgccagcat ccgcagatca
    10561 ccctgccgga ggttgactcc tttgccgccg ccgctgagaa ggatctcgga gccgcctggc
    10621 tgaaagacca cgcccatccc gcccggtaat cccctgccgc catgaaagcc ctgtcattcc
    10681 tccccctgct cgccgcagcc tctgcccacg ccgccccgct cgacacgccc ctcgccgcca
    10741 tccgcgccgt cgggcgcgag ggcgcgggca atgaaaacgc ctccgccgcc tggcggcagg
    10801 tctcgcaggc ggatgccgcc gcgctgccgg aaatcctcaa gggcatgaac ggcgccaacc
    10861 ccctggcaga gaactggctg cgcgccgccg tcggtgccgt ggcggacaag gccatcgccg
    10921 ccaaaacgct gcccgtcccc gccctccagg ccttcctcat ggacaccgcc aacagccccg
    10981 gcccgcgcgt gctggcattc gacctcatcc agcgcgccga ccccgcgctg gccggaagca
    11041 tcaccgcctc cctgcttgac gaccccagca acgagctgcg ccgccacccc gtggccagtc
    11101 tcatccaggc cggggacgag gcgctggccc agggcaaaaa agacgccgcc accgccgcct
    11161 accagcaggc catgcgcggc gcgcgcgacg aggaccagat caaggatctg gcgaaaaaat
    11221 taaaagacct cggccagccc gtggacctgc cccggcactt cgggttcatc atgcattgga
    11281 agctcatcgc ccccttcacc aacgccgagc gcaaaggctt cgacaccgtc ttcccgcccg
    11341 agaaagagat cgtcctcgac gccgagtatg agggcaaagg cggcgacaag gcgcgctggc
    11401 aggacttcac cagccaggac gactacggca tggtggactt caacaagccc ttcaccatgc
    11461 tcaaggaggt gaccggctac gccttcaccg agttccactc cgccgaggag cgcgacgcgc
    11521 aaatccggct tggctgcaaa aacggctgga aggtgtggtt caatggcgag ctgctcttcg
    11581 cccgcgacga gtaccaccgc ggcgccaagc tggaccagta caagctgccc gtccgtctga
    11641 ggaagggcaa aaacaccatc ctcgtgaaat gctgccagaa cgagcagaag gagacctgga
    11701 ccgtcgagtg gcagttccag ctccgcgtct gcgatgccac cggcaccgcc atccaccccg
    11761 tgccctgacg ccagcccccg gccatgcgca acaccctgct cattttcctc ggcggcggtc
    11821 tcggctccgt gctgcgcttc tggaccgtgc tggccgcgca gcgctgctgg ccgggcgcgg
    11881 tcttccccgc cggcgtgctc gtggcgaacc tgctgggcag cttcatcctc ggcttccttt
    11941 tcgcctggcc ggcgatgagg gcgcgcgacg ccggggcgtg gttcttctgc gccaccggcg
    12001 tgctgggcgg ctacaccacc ttttccaccc tggccggcga ctccctccag ctcctgctca
    12061 acaaccaccc tctcccggcc ctgctcaacg ccttcggcag catcaccgcc ggcctgctcg
    12121 ccgccgcgct gggctggcag acggcgcggt tcattttcgg ctgaatcctc accttcccag
    12181 gtaaagatcc cgcccgcgcg ccagggcctc gatcttccgg tcggcgatgc tgcgcttgag
    12241 catccagaag ccgcagtcgg ggtggacata cttcacgcgg cccgcgccca gcctgcgctc
    12301 cgcgcgctcg atgcggcggg cgatctcctc cgcgctttcg atgtggttga ccttgatgtc
    12361 caccacgccg atgccgacgc ccacgcgcgc ctccagctcc cgcagcgcgt ccaggtccgc
    12421 ctccggccgg tgcgccagct ccagcaccag gtggtccgcg cgcaggctgt tgaggaagtc
    12481 caccagcgcc gcccagccgc ctttctggat ggtctggccg ccgtagttgc cgaagcagaa
    12541 atgcaccgcc ttctccccgc gcgcggcgtc gagcacgcgg ttgatgccgc gcgcggccag
    12601 gggcgcgtcc gccgggttgc cgggcacgtt ggcctc
//
LOCUS       DAOMYJ010000154        12486 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Prosthecobacter sp. isolate AMR_MDS_5629
            NODE_1696_length_12486_cov_21.243102, whole genome shotgun
            sequence.
ACCESSION   DAOMYJ010000154 DAOMYJ010000000
VERSION     DAOMYJ010000154.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867850
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prosthecobacter sp. (wastewater metagenome)
  ORGANISM  Prosthecobacter sp.
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Verrucomicrobiaceae; Prosthecobacter.
REFERENCE   1  (bases 1 to 12486)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12486)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.30
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:34:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,742
            CDSs (total)                      :: 4,684
            Genes (coding)                    :: 4,676
            CDSs (with protein)               :: 4,676
            Genes (RNA)                       :: 58
            tRNAs                             :: 55
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12486
                     /organism="Prosthecobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1696_length_12486_cov_21.243102"
                     /isolate="AMR_MDS_5629"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1965333"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1021
                     /locus_tag="PLP58_15065"
     CDS             <1..1021
                     /locus_tag="PLP58_15065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ09896.1"
                     /translation="ARLAAAPARGDAELVERAYQVTLSRRPSDTEKQEAAAFLQTQRR
                     QLQREAPPKPPVASPLASASDYFGPSAASKTAKTLVLQPGTPHEKLRVKETGRIEGSD
                     FSIEAVVNLSSLYPDASVRTIASRWDNSKTDRGWALGVTSEKSAYKPNNLIVQLVGED
                     FQGSLAYEVVASDIRIPAGKPWYVAAIINNDPEPDQKFGGTVTFYARDLSDASAPMQT
                     ATVQHQMVGGYVNPDRPLLIGGRDKDKRSLWHGAIARVALRHGAPEPGRLMAWAGAGD
                     ASCVADISADSLAEMLKGDPKTAWRWETTSPAVTAKGAPDPDREALTDLCHILLNANE
                     FFYLH"
     gene            complement(1156..3285)
                     /locus_tag="PLP58_15070"
     CDS             complement(1156..3285)
                     /locus_tag="PLP58_15070"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HRJ09897.1"
                     /translation="MKLSLILIALFGCSALSHGQTADATATPPASSATLDTVLVYGEA
                     EDDAVIQHPFPLAVEGNKIFAGKRATVIDLDALPKVQANNYRQALALTPGLLFSEETT
                     PLVSIGYRGIGEPHRMQFMQVLKDGVPIHADPFGYPEAYYTPPLDTVDRIEFIRGGGS
                     LMYGPQPGGAFNYVTYMPQRNSPFSFRTQNIFGSDELFSSYTAVDGTLGQLGYLGYFN
                     HRESQGFRERNSQYKLNGGHLKLVWDFDDDTRLIFGFDGYEEEHGEPGGLTARDFARN
                     PERTTRNHDEFRLRRYIGNVELQHRIQTGTELNVKAWGGYYQRWSKRQRGGGFGTRPA
                     GGASLTNNIENQEFYSFGIEPRLRHDWEGLGGQHTLAAGMQFYYVDSPREDKRGRSFD
                     AEDGVTFLDSERSTTYGSFFAENKFTYGKFSITPGFRMELVKQDVHSRNFNATTGVPT
                     GEVTKSKLDSQPLFGLGMALDFDNQTQLYANVSQSYRTTIFTESIVAQPGLTATDTDP
                     SLGWSYEIGYRGTPREWLAFDTSLFLIDLDNRFGVSGGQLRSVGRSINYGWDGAVQFD
                     IIGGLDAARGTKLADSLGALNLYANVSLLEAELHGGPNDGATPQYAPDFILRTGMIYK
                     KKGVKVSFLGTFVDDHNARDDADPRFAIPGYMTWDLTAEIPVTENLNLMAGINNVFDE
                     SYYSRVRNDGIDPGYGRNFYVGGSVSF"
     gene            3551..5347
                     /locus_tag="PLP58_15075"
     CDS             3551..5347
                     /locus_tag="PLP58_15075"
                     /inference="COORDINATES: protein motif:HMM:NF012863.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDSL-type esterase/lipase family protein"
                     /protein_id="HRJ09898.1"
                     /translation="MAAPKLVLAGDSTVATVSKPRKDRPDLAGWGQMLPDFLTPEATV
                     VNHARGGTSTKSFRALGLWDRVLAEKPDYVLIQFGHNDQPGKGPERETDPAGSYRENL
                     RQFIREARAIQARPVLVTSVARRVYENGRLTSSLGPYAEAVKAVASETGVPVVDLHRT
                     SFLLFDQRGEKFSQQTYGPSAEDRTHFNSEGARMVARLVAEQLQRVVPELAPHIRLVP
                     PPPAGAPFEVKLTTVTQGYDGETCWVHPRAGAIPGATPTVVLTMQKLLMSGSDIFYAL
                     NDTRTGDLGQTWSPIRQHDDTLGRRQEPGGVVIAASDFTPKWHAQSGRLLGIGHTVRY
                     AGDKVMHNRPRETSWSVYDETTRTWSAWDTLKLPDEAKFFNCGAGSVQRVDLDNGEIL
                     LPVYLKAEADKYYRVTVLRCAFDGKELRYLGQGGELFLESGRGVYEPSLTRFQGRFYL
                     TLRNDTDGYVAVSDDGLNYGPIQPWRYTDGESLGNYNTQQHWISRPDALYLVYTRKGA
                     RNDHVFRHRAPLFMAQVDVEKMAVMRGTETVLVPERGARLGNFAIAEVSENETWVTVA
                     EWMQCMSPNVIIPPENPFGADNSVFAARILWR"
     gene            5437..6546
                     /locus_tag="PLP58_15080"
     CDS             5437..6546
                     /locus_tag="PLP58_15080"
                     /inference="COORDINATES: protein motif:HMM:NF019843.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HRJ09899.1"
                     /translation="MRNLRRIFASAAIVLFAAAAPAQNERVVEELQGWAEKGDSDAQF
                     ELGLRMLTGEGLKKDEKKGAEWLEKAANQKHLRAQFLTGSLYEDGIGVKRNDALAFEW
                     FRRAAENGFAPAQHAVAMGYDLGKGVKQDPAAAAEWLEKAAAQSHAPSQTALAAKYER
                     GVGVAKSPAKAALYYLRAAQQDFVPAMSRLANLYYSGNGVPVDYRRAGAWYQRAARSD
                     DPWAANNIAWFLATCPDETLHNGEMAVQFATRALRIMDEAGEEQRYEMLDTKAAALAR
                     NGEYLEALLWQKKSIALLKEDTEISEEERQTLMKEFEDRMKLYQKQAPYTEPEPKGEA
                     DTAPLPNDSILQEEGLPGGDSKPKSKKGKSKGTVV"
     gene            complement(6553..7626)
                     /locus_tag="PLP58_15085"
     CDS             complement(6553..7626)
                     /locus_tag="PLP58_15085"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009958456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070573 - metallodipeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane dipeptidase"
                     /protein_id="HRJ09900.1"
                     /translation="MLTFDAHLDLSLNALGGFNRDLRLPVHEIRRCEAGMTGKGRAGG
                     TTAFPEMRQGRVGICVATQLAGCMPGVTPIANWMSPEQAYAETQGQLAWYRAMEEQGH
                     MRQIVDLPGLEKAVADWSDTSEPDDSKPIYYILSLEGADSIRSVGHLEKHWEQGLRAM
                     GPAHYGVCRYAMGHDTPGGLPACGRDLIREMDRLGMILDVTHLSDESFWEALDIFDGA
                     VWASHSNCRALVPDPRQFSDEQIKALIGRGAVIGAALDAWMMIPGWIRGTTTPQETGL
                     KLEVICDHMDHVCQLAGNALHCGIGTDLDGGYGTEQTPEDLDTIADLARIPAMLEKRG
                     FSADDIAGIMHGNYLRLLRGAWA"
     gene            complement(7694..9394)
                     /locus_tag="PLP58_15090"
     CDS             complement(7694..9394)
                     /locus_tag="PLP58_15090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007413084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004819 - glutamine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006425 - glutaminyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--tRNA ligase/YqeY domain fusion
                     protein"
                     /protein_id="HRJ09901.1"
                     /translation="MSDTPAAPASQDFIREMIAADLAQGRNDGWVITRFPPEPNGYLH
                     IGHAKSICLNFGLALEHAPKSRCHLRFDDTNPTKEEVEYVDSIMEDVRWLGFDWGRHL
                     YYASDYFEKLYAFAEQLIGKGLAYVDDQTPEQIRATRGTLTSPGEHSPFRGRPPAENL
                     DLFRRMRAGEFKEGERVLRAKIDMASPNMNLRDPVIYRILHAHHHRTGDAWRIYPMYD
                     YAHPLSDALEGITHSLCTLEFEHHRPLYEWLVNNVDGLPGAPCQREFARLNLTFTVMS
                     KRKLLRLVKEGFVGGWDDPRMPTISGIRRRGYTPAAVRAFCKTIGLTKYNSLTDLQLL
                     EHCVREDLNKTARRAYAVLRPLKVVLTNYPEGRVEHVELVNNPENEAEGKRLVPLSRE
                     LYIEADDFMEEPVPGFHRLKPGGEVRLKFAFCIICHEVVKDAAGQISELRCTYDDTTR
                     HGAKPEGRPKPKGTIHWVSVAHAVDVEARLYEKLFTVEQPDGAAGEDGDFTRFLNPHS
                     IEMVTAKAEPSLAEAEAGSHFQFERVGYFFTDPKDSTPGRPVFNRTVALKDGFAKGKA
                     "
     gene            complement(9477..9959)
                     /locus_tag="PLP58_15095"
     CDS             complement(9477..9959)
                     /locus_tag="PLP58_15095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009960323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HRJ09902.1"
                     /translation="MDARIKERLEAHIVATGLRRTKQRETIVEAAFATDDHFNADELL
                     EKARKLDRTVSRATVYRTLQLLVECGLLRIVDLGRDQTYYDPNFLDKPQHNHLICLDC
                     DRVVEFEDEHSALLHDCITRRLGFQPQMKSMRIEARCDELANTGHCKYKDEREKALAR
                     "
     gene            complement(10026..11474)
                     /locus_tag="PLP58_15100"
     CDS             complement(10026..11474)
                     /locus_tag="PLP58_15100"
                     /inference="COORDINATES: protein motif:HMM:NF023459.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3520 domain-containing protein"
                     /protein_id="HRJ09903.1"
                     /translation="MNPHPHENPDLTAYSLGELDAQHAAQVHGLLRISPTAASEMEQI
                     EAVAQALSQTAPVPVDRLHPAQRRAVLNPAQMPRMMAPLMPRPMPARRRSAWPVLAGM
                     MKAAAVIALSLAGYWVGRHSDWMSSAPSIAVDDASIKGVPSVENPPLVAATTEPQLEL
                     AKPAPVEMPVTPDEEVKPVEIVIVEEKAAPPAAVEVAAASPATDQSATEPVVLVESPP
                     NTPAAPAPVVRSLTRPAAGTAFVSTTRQAVSQFALRPADIRPAPPQPAPGQTFAAPMT
                     ARPEVRDEAKPPRAPELYIHSWKADVAVCPWNPAHRLMRVVIQLPADQPAATQSFSYP
                     LHVAFDPNNVREYRQLCERHLSAGELRSAGTHSLWYEYLPNGKSEPGKNVATITLPQG
                     RFTTQTVGPFDGSKLSVTERGLDWRSAREDFIFDTAVVGFGLLLRGVNQSPALNHQLV
                     LTLAEKTLASDSTGERRRFVRQVREAKAAAGL"
     gene            complement(11471..12025)
                     /locus_tag="PLP58_15105"
     CDS             complement(11471..12025)
                     /locus_tag="PLP58_15105"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HRJ09904.1"
                     /translation="MPVQPNPDEASLLVRRALDLHESALIAYSAGILNGDVERAREVV
                     QDSLLKLYLTEPAKVQDNLKAWLYTVCRNRALDILRKDQRLDLGNEDALGLAASFDPD
                     PSEHADSHEMHARVWELVEKLRPNQREVIRLKFMHDCSYQEIAEVTGLSTGNVGFIMH
                     TAIKKLRQMMQRELATRERTPTPS"
     gene            12165..>12486
                     /locus_tag="PLP58_15110"
     CDS             12165..>12486
                     /locus_tag="PLP58_15110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ09905.1"
                     /translation="MKTPLLSLILALASLAGLRAQNLETWVLTDGRMFEAQVKQVVPG
                     TVTFTLRTGADQPLEIAKLSERSKKQLAEVLGLGQSVVTPLSPVAAATAPAATPSATP
                     MPAAA"
BASE COUNT         2267 a         3876 c         4108 g         2235 t
ORIGIN      
        1 ggccaggctc gccgccgcgc ccgctcgcgg cgacgccgaa ctggtcgagc gcgcctacca
       61 ggtgacgctc tcccgccgcc cctcggacac cgaaaagcaa gaagccgccg ctttcctgca
      121 aacccagcgc cgccagctcc agcgcgaagc cccgcccaag ccgcctgtgg ccagccccct
      181 ggcatccgcg agtgattatt ttggcccatc cgccgcctcg aagaccgcca aaacgctcgt
      241 gctccagccc ggcaccccgc atgaaaaact gcgcgtcaaa gaaacaggcc gcatcgaggg
      301 cagcgacttt tccatcgagg ccgtcgtgaa tttgtccagc ctctatcccg acgcctccgt
      361 gcgcaccatc gcctcccgct gggacaacag caaaaccgac cgcggctggg cgctcggcgt
      421 caccagtgaa aagtccgcct acaaacccaa caacctcatc gtccagctcg tcggtgagga
      481 tttccagggc agcctggcct atgaagtcgt ggcctcggac atccgcattc ccgccggcaa
      541 accctggtat gtcgccgcca tcatcaacaa cgaccccgaa ccggaccaga aattcggcgg
      601 caccgtcact ttctacgcgc gcgatttgag cgacgcctca gcgcccatgc agaccgccac
      661 cgtgcagcat cagatggtgg gcggttatgt gaatcccgac cgcccgcttt tgatcggcgg
      721 gcgtgacaag gacaagcgca gcctctggca cggtgccatc gcccgcgtgg ccctgcgcca
      781 tggcgcgccg gaaccgggcc ggctcatggc gtgggccggc gccggtgacg cctcctgcgt
      841 ggctgacatc agcgccgact ccctcgccga aatgctcaaa ggcgatccca aaaccgcctg
      901 gcgctgggaa accacgtcac ccgcggtcac cgccaaaggc gcccccgacc ccgaccgcga
      961 ggccctgacc gacctgtgcc acattcttct gaatgcgaac gagttctttt atctgcattg
     1021 aaccttccgc aggggctcat ggcagcgcgg ggaaacctgc ggtcccggct tgctcccgcc
     1081 cgcacgcggg agaagtgccc gcaaaaaaat agcgcggatg cttccatccg cgccttgaag
     1141 taagcgaggc aggaattaga aactcaccga gccgccgacg tagaagttgc gcccgtagcc
     1201 cgggtcaatg ccgtcattgc gcacacgcga gtagtagctc tcgtcgaaga cgttgttgat
     1261 gccggccatg aggttaagat tttcagtgac ggggatttcg gcggtgaggt cccaggtcat
     1321 gtagccgggg atggcgaagc gcgggtcggc gtcatcacgc gcgttgtggt cgtccacaaa
     1381 ggtgccaagg aaggagacct tgacgccctt ctttttgtaa atcatgccgg tgcgcagaat
     1441 gaagtcgggg gcgtattgcg gagtggctcc atcgttcggg ccgccgtgga gttcggcttc
     1501 gaggagtgag acgttggcat agagattgag cgcgcccagg ctgtccgcga gcttggtgcc
     1561 gcgcgcggcg tcgagcccgc cgatgatgtc gaactgcacg gcgccgtccc agccgtagtt
     1621 gatgctgcgg ccgacgctgc ggagctggcc gccggaaaca ccgaagcggt tgtccaggtc
     1681 aatgaggaag agactggtgt cgaaggccag ccactcgcgc ggcgtgccgc ggtagccaat
     1741 ctcatagctc cagccaaggg aggggtcggt gtccgtggca gtgaggccgg gctgggcgac
     1801 gatggactcg gtgaagatgg ttgtgcggta gctctgggag acgttggcgt agagctgggt
     1861 ctgattgtcg aagtccagcg ccatgccgag gccgaagagc ggctgggagt ccagtttgct
     1921 tttggtcacc tcgccggtgg gcacgccggt ggtggcgttg aaattccggc tgtgcacgtc
     1981 ctgcttgacc agttccatgc ggaaaccggg agtgatggag aacttgccgt atgtgaattt
     2041 gttctcggca aagaaggagc cgtaggtggt gctgcgctcg gaatcgagga aggtgacgcc
     2101 atcctccgcg tcgaagctgc ggccccgctt gtcctcgcgg ggggagtcca cataatagaa
     2161 ttgcatccca gccgccaggg tgtgctggcc gccgaggccc tcccagtcat ggcgcagacg
     2221 cggctcaatg ccgaagctgt aaaactcctg gttttcgatg ttgttggtga gggaggcgcc
     2281 gcctgcgggc cgggtgccga agccgccgcc gcgctggcgc ttgctccagc gctggtaata
     2341 gccgccccag gctttgacat tcagttccgt gccggtctgg atgcggtgct ggagttcgac
     2401 gttgccgatg tagcggcgca ggcggaactc gtcatgattg cgcgtggtgc gctcggggtt
     2461 gcgggcaaag tcgcgcgcgg tgaggccgcc aggctcgccg tgctcctcct cgtagccgtc
     2521 gaaaccgaaa atgagccgcg tgtcgtcatc gaaatcccac acgagtttga gatggccgcc
     2581 attgagtttg tactggctgt tgcgctcgcg gaaaccctgg gactcgcggt gattgaagta
     2641 accgaggtag ccgagctggc cgagcgtgcc gtccacggcg gtgtagctgg agaatagctc
     2701 gtcgctgccg aagatgttct gcgttcggaa ggagaagggc gagttccgct gcggcatgta
     2761 agtcacatag ttgaaggcgc cgccgggctg cgggccatac atgagggagc cgccgccgcg
     2821 gatgaactcg atgcggtcca ctgtgtcgag aggcggggtg tagtaggctt cggggtagcc
     2881 gaagggatcg gcatggatgg gcacgccgtc tttgagcacc tgcatgaact gcatccggtg
     2941 cggctcgccg atgccgcggt agccgatgct gaccagcggc gtggtttcct ctgagaaaag
     3001 caggccggga gtcaaagcaa gcgcctggcg gtaattattc gcctgtactt tgggcagcgc
     3061 gtcgaggtca atgaccgtgg cgcgcttgcc agcaaagatt ttattgcctt ccacggcgag
     3121 gggaaagggg tgctgaatga ccgcatcgtc ctcggcctcg ccatagacaa ggacagtgtc
     3181 gagagtggcg gagctggctg gcggcgtggc ggtggcatcg gcagtctggc cgtgggagag
     3241 ggccgaacag ccgaagaggg caatgaggat aaggctgagt ttcatgagag gttgactgag
     3301 gctaatgagt tccattcgca attggagagg cgcgcatttt atctcgattc aaaatcgcgc
     3361 aagcagaatt ttgcgattga ttcgcatttg ctcgtagcgc gccatttttt tccctccgct
     3421 caaaaaaaat ggggcgagcc ccactgaatg ggagtgcatt tcatcgagag cacacaggca
     3481 cacaggccgt tctgtgccga tgcaaaaatg gattctcttt gccctggcga ctggtttgac
     3541 aacttctgga atggccgccc cgaagctcgt tctcgccggt gactcgacgg tggccaccgt
     3601 ctcgaagccg cgcaaagacc gcccggacct cgcgggctgg gggcagatgc tgcccgactt
     3661 tctcaccccg gaggccaccg tggtgaacca tgcccgcggc ggcaccagca cgaagagctt
     3721 tcgcgcgctc ggcctatggg accgcgtgct cgccgagaag ccggactatg tgctcattca
     3781 gttcggccac aacgaccagc ccggcaaagg ccccgagcgc gagaccgacc ccgccggcag
     3841 ttatcgggaa aatctgcgcc agttcatccg cgaagcgcgc gccatccagg cgcggcccgt
     3901 attggtgact tccgtggccc ggcgcgtgta tgaaaacggg cggctcacct ccagcctcgg
     3961 accttatgcg gaggcggtca aagccgtggc gtcggagacc ggcgtgcccg tggtggacct
     4021 gcaccgcacc agctttctgc tctttgacca gcgcggggaa aaattcagcc agcagaccta
     4081 cggcccctcc gcggaggacc gcacgcactt caacagcgaa ggcgcgcgca tggtggcgcg
     4141 tctcgtcgcg gagcagctcc agcgcgtcgt gcctgagctg gccccgcaca tccgcctggt
     4201 gccgccgccg cccgcgggag cgccttttga agtcaagctc accaccgtca cccagggtta
     4261 tgacggcgag acctgctggg tgcatccgcg cgcgggcgcc atccccggtg ccacgcccac
     4321 ggtcgtcctc accatgcaga agctgctcat gagcggcagc gacatctttt acgcgctcaa
     4381 tgacacgcgc accggcgatc tcggccagac ttggagcccc atccgccagc atgacgacac
     4441 cctgggccgc cgccaggagc ccggcggagt cgtcatcgcc gccagcgatt tcaccccgaa
     4501 gtggcacgcc cagagcggca ggctgctcgg catcggccac accgtccgct atgccggcga
     4561 caaggtcatg cacaaccgcc cgcgcgagac gagctggtcc gtctatgatg aaacaacgcg
     4621 cacttggtcc gcctgggaca ccctgaagct gccggacgag gccaagtttt tcaactgcgg
     4681 cgccggcagc gtccagcgtg tggacctgga caatggcgag atactgcttc ccgtttacct
     4741 caaggccgag gccgacaaat actaccgcgt caccgtcctg cgctgcgcct tcgatggcaa
     4801 agagctgcgc tacctcggcc agggcggcga gcttttcctc gagtccggcc gcggcgtcta
     4861 tgagccttcg ctcacgcgct tccagggccg cttttacctc accctgcgca acgacaccga
     4921 cggctatgtg gccgtgagcg acgacggcct gaactacggc cccattcagc cctggcgcta
     4981 cacggacggc gaaagcctcg gcaactacaa cacccagcag cactggatct cccgcccgga
     5041 cgccctctac ctcgtctata cccgcaaagg cgcgcgcaat gaccacgtct tccgccaccg
     5101 cgcgcctctc ttcatggcgc aggtggatgt ggaaaaaatg gccgtcatgc gcgggacgga
     5161 gaccgtgctc gtgcccgagc gcggcgcgcg gctggggaac ttcgccatcg ccgaagtcag
     5221 tgaaaacgag acctgggtca ccgtggccga gtggatgcag tgcatgagcc cgaacgtcat
     5281 catcccgccg gagaacccct tcggcgcgga caacagcgtc ttcgcagcgc gcatcctctg
     5341 gcgctgagaa cgcctcacag cctcggattg attcgagcaa cccgcccgtc actttgtttg
     5401 ttgtagtgcc agtttcaaaa acgtatgatt tccgccatga gaaacctccg ccgcattttc
     5461 gcatccgctg ccattgtgct tttcgccgcc gccgctcccg cccaaaacga acgggtggtc
     5521 gaggaactcc agggctgggc cgagaagggg gattccgacg cccagttcga gctgggactg
     5581 cgcatgctga ccggggaagg cttgaaaaag gatgagaaaa aaggcgcgga atggctcgag
     5641 aaagcagcca accaaaagca cctgcgcgcg cagtttctca ccggatcgct ctacgaggac
     5701 ggcatcggcg tcaagcgcaa cgacgcgctg gccttcgagt ggttccgccg ggcggcggaa
     5761 aacggcttcg cccccgccca gcatgccgtg gcgatgggtt acgacctcgg caagggagtg
     5821 aagcaggatc ccgcagccgc cgcagagtgg ctggaaaaag ccgccgcgca aagccacgcc
     5881 ccctcccaga cggccctggc cgccaaatat gagcgcggag tcggagttgc caaaagtccc
     5941 gccaaagccg cgctctatta cctgcgcgcc gcgcagcagg atttcgttcc cgccatgagc
     6001 cgtctggcca atctatacta cagcggcaac ggcgtgccgg tggactaccg ccgcgccggc
     6061 gcctggtacc agcgcgccgc ccgcagtgac gacccctggg ccgccaacaa catcgcctgg
     6121 ttcctcgcca cctgccctga cgagacgctg cacaatggcg agatggccgt gcagttcgcc
     6181 acccgcgcgt tgcgcatcat ggacgaggcg ggagaggagc agcgctacga gatgctcgac
     6241 accaaagccg ccgccctggc ccgcaacggc gaatatttgg aagcccttct ctggcagaaa
     6301 aaatccatcg ccctgctcaa ggaggacacc gaaatctccg aggaggagcg acagactctc
     6361 atgaaagagt ttgaagaccg catgaagctc taccaaaagc aggctcctta cacggagccg
     6421 gaacccaagg gtgaagccga caccgcgcca ctgcccaacg acagcattct gcaggaggag
     6481 gggctgcccg gaggcgactc caaacccaag agtaaaaaag gcaaaagcaa gggaaccgtg
     6541 gtctgagcgc gctcaagccc aggccccgcg aagcaggcgc aggtagttgc cgtgcatgat
     6601 gccggctatg tcatcggcgc tgaaaccgcg cttttccagc atggcgggga tgcgcgccag
     6661 atcggcgatg gtgtccaaat cctccggcgt ctgctcggtg ccgtagccgc cgtccaaatc
     6721 ggtgccgatg ccgcagtgca gggcattccc ggcgagttgg cagacatgat ccatatgatc
     6781 gcagatgact tcgagcttga ggcccgtctc ctgcggagtg gtggtgccgc gaatccagcc
     6841 cggaatcatc atccatgcgt caagcgcggc gccaataacc gcgccgcgcc cgatgagcgc
     6901 cttgatctgc tcgtcggaga actggcgcgg atcgggaacc agcgcgcggc agttgctgtg
     6961 gctggcccag acggcgccat cgaagatatc cagcgcctcc cagaaactct catccgacag
     7021 atgcgtcaca tccaggatca tgccgagacg gtccatctcc cggatcaagt cgcgcccgca
     7081 ggccggcaac ccgccgggcg tgtcatgacc catggcgtaa cggcagacgc cgtagtgcgc
     7141 cggccccatg gcgcgcagcc cctgctccca gtgcttttcc agatgcccga cgctgcggat
     7201 gctgtccgca ccctccagac tgaggatgta gtagatgggt tttgaatcgt ccggctccga
     7261 ggtgtccgac cagtccgcca ccgctttttc cagtcctggc aaatccacga tctggcgcat
     7321 gtgcccctgc tcctccatgg cgcgatacca ggcgagctgg ccctgagtct ccgcgtatgc
     7381 ctgctcgggc gacatccaat tggcgatggg agtgacgccc ggcatgcaac cggcgagctg
     7441 ggtggccaca cagatgccca ccctgccctg gcgcatttca ggaaaagccg tcgttccccc
     7501 cgcgcgcccc ttgccggtca ttccggcctc gcaccggcgt atttcgtgga caggcaggcg
     7561 cagatcgcgg ttgaagccgc cgagggcgtt gagactgagg tcgaggtggg cgtcaaaggt
     7621 gagcatggaa ggtgctgaat agtcgcggcg gaccggcgcg ctgtttcaaa agaagagccg
     7681 catgaccgcg cgctcacgcc ttgcctttgg cgaagccgtc cttgagagcc actgtgcggt
     7741 tgaagaccgg cctgccgggc gtgctgtcct ttgggtcggt gaaaaagtag cccacgcgct
     7801 caaactgaaa atgcgatccg gcttctgctt cggcgagcga aggctccgcc ttggcggtga
     7861 ccatctctat ggagtgaggg ttgagaaacc gtgtgaaatc cccgtcctcg cccgcagccc
     7921 catcaggctg ctcgaccgtg aaaagcttct cgtagagccg cgcctcaacg tccaccgcat
     7981 gcgcgacgct gacccagtgg atggtgcctt tcggctttgg gcgcccctcg ggcttcgcgc
     8041 cgtggcgggt ggtgtcatcg taggtgcagc gcagctcgct gatctggccg gcggcgtctt
     8101 taaccacttc atggcagatg atgcagaagg cgaatttaag ccgcacctcg ccccccggct
     8161 tgagccggtg gaaaccgggc acaggctcct ccatgaagtc atccgcctcg atgtacagct
     8221 cgcggctcag cggaacgagg cgctttcctt cggcttcgtt ttccggattg ttcaccagtt
     8281 cgacgtgctc cacccgtcct tcgggatagt tggtgagcac gactttgagc gggcgcagca
     8341 ccgcatacgc gcgccgggcg gttttgttca aatcctcgcg cacacaatgc tccagaagct
     8401 ggaggtcggt gagggagttg tatttggtga ggccgatcgt tttgcagaag gcgcgcaccg
     8461 cggcaggagt gtatccgcgc ctgcggatgc cgctgatggt gggcatgcgc gggtcatccc
     8521 agccgccgac gaagccctcc ttcaccaggc gcagcagctt gcgcttgctc atcacggtga
     8581 aggtcaggtt cagacgcgcg aattcgcgct ggcagggcgc gccgggcagg ccgtccacgt
     8641 tgttgacgag ccattcgtaa agcggccggt gatgctcgaa ctcgagcgtg caaagactgt
     8701 gcgtgatgcc ctccagcgcg tcgctcagcg gatgcgcgta atcatacatc ggatagatgc
     8761 gccaggcgtc gccggtgcgg tgatgatgcg cgtgcaggat gcggtagatg accgggtcgc
     8821 gaaggttcat gttgggcgag gccatgtcta tcttcgcgcg caggacgcgc tcgccctcct
     8881 tgaactcacc cgccctcatg cggcggaaca gatcgagatt ctccgcgggg ggacgcccgc
     8941 ggaacgggct gtgctcgccg gggctggtga gcgtgccgcg ggtggcgcgg atttgctcgg
     9001 gcgtctggtc gtccacgtag gccaggcctt tcccaataag ctgttcggcg aatgcgtaga
     9061 gcttctcaaa gtagtcgctg gcatagtaca gatgcctgcc ccagtcgaag ccgagccagc
     9121 gcacgtcctc catgatggag tccacatact ccacctcctc cttggtggga ttggtgtcgt
     9181 caaaacgcag atggcagcgg gacttgggcg cgtgctccaa agccagtccg aaattcaggc
     9241 agatgctctt ggcgtggccg atgtgcaggt agccgttggg ctcgggcggg aagcgtgtga
     9301 tgacccagcc gtcattgcgc ccctgcgcga gatcggcggc gatcatttcg cggatgaaat
     9361 cctgggaggc gggggcggcg ggtgtgtcgg acatgggcgg ggcggatgtt tgccggagcg
     9421 agctaaggag tcaaggagcc agggaatgca gccctgcccc gtcaatgccg cgcggatcat
     9481 ctggcgaggg ccttttcccg ctcgtccttg tatttgcaat ggccggtgtt ggccagctcg
     9541 tcacaacgcg cctcaatgcg catggacttc atttgcggct gaaaaccgag acggcgggtg
     9601 atgcagtcat gcagcagggc gctgtgctcg tcctcaaact cgacgacgcg gtcgcaatcc
     9661 aggcagatca ggtggttgtg ctgcggtttg tcgaggaaat tggggtcgta gtaagtctgg
     9721 tcgcgtccga ggtccacgat ccgcaacagg ccgcattcga cgaggagctg cagggtgcga
     9781 tagacggtgg cgcgggagac agtgcggtcg agtttgcggg ctttttcgag aagctcgtcg
     9841 gcattgaagt ggtcgtctgt ggcgaaagcg gcttcgacga tggtctcgcg ctgcttggtc
     9901 cgccgcaagc cggtggcgac gatgtgcgcc tccagccgct ctttgattct ggcatccatg
     9961 ttgagacgag gctagactcg ttgagtctca acgcaagcct tgcttttgag aattgaatcc
    10021 ttatttcaca ggccggctgc ggccttggcc tcgcgaactt gacggacaaa gcggcggcgc
    10081 tcgcccgtgc tgtcagaagc gagggttttc tcggcgaggg tgagcacgag ttgatggttg
    10141 agtgcggggc tctgattcac acctcggagc agcaggccga atccgaccac cgcagtgtcg
    10201 aagatgaagt cctcgcgcgc ggagcgccag tcgagtccgc gctcggtcac gctgagcttg
    10261 gagccgtcaa agggtcccac ggtctgcgtg gtgaagcggc cctggggcag ggtgatggtg
    10321 gcgacgtttt taccaggctc ggatttgccg tttggcagat actcatacca gaggctgtgg
    10381 gtgccggcgc tgcgcagttc ccccgcagac agatggcgct cgcaaagctg gcggtattca
    10441 cgcacgttgt tgggatcgaa agccacatgg agggggtagc tgaaagactg ggtggctgcg
    10501 ggctggtcgg cgggcagttg aatgaccacg cgcatgagac ggtgggcggg attccaaggg
    10561 cacacggcca catccgcctt ccaagagtgg atgtagagct cgggcgcgcg cggcggcttc
    10621 gcttcgtccc tgacctccgg cctggccgtc atgggtgcgg caaaagtctg gcctggcgcg
    10681 ggctgcggag gcgcgggccg gatgtccgca gggcgcaggg caaactggct caccgcctgg
    10741 cgtgtcgtgc tgacaaaagc ggtgccggcc gcaggccggg tcagggatcg cacgacggga
    10801 gcgggagcag caggggtgtt gggcggggac tccaccagaa cgaccggctc agtggcggat
    10861 tgatccgtgg cgggtgatgc ggctgccacc tcgacagcgg caggcggcgc ggctttttcc
    10921 tccacgatga cgatttccac tggcttcact tcctcatctg gagtgactgg catctccacc
    10981 ggggcaggct tggcaagctc aagctggggc tcggtcgttg ccgcgaccaa gggcgggttc
    11041 tcgaccgacg gcaccccttt gatggaagca tcatccaccg cgatggaagg cgcggagctc
    11101 atccaatcgg aatgcctgcc gacccagtag ccggccaatg acagggcgat gacagcggcc
    11161 gctttcatca tcccggcgag gacaggccat gcggaacggc ggcgcgccgg catggggcgg
    11221 ggcatcaacg gagccatcat gcgcggcatt tgagcgggat tcaaaaccgc gcgacgctga
    11281 gcgggatgca ggcggtccac cggcaccggc gcggtctggc tcaatgcctg tgcgacggcc
    11341 tcgatctgct ccatttcgga cgcggcggtg ggcgagatgc gcagcaggcc gtggacctgg
    11401 gcggcgtgct gggcatccag ctcgccgagg gagtaagccg tgaggtcggg gttttcgtgg
    11461 gggtgcggat tcatgaaggg gtgggggttc gttcgcgtgt ggccagctcg cgttgcatca
    11521 tctggcgcag ctttttgatg gccgtgtgca tgatgaagcc gacattgccc gtgctgaggc
    11581 cggtgacttc ggcgatctcc tggtaggagc agtcatgcat gaatttgagg cggatgacct
    11641 cgcgctgatt cggacgcaat ttttccacca gctcccagac gcgggcgtgc atttcgtggg
    11701 agtcggcgtg ctccgagggg tcgggatcaa aggaggcggc caggccgagg gcgtcctcgt
    11761 tgccgaggtc gagccgctgg tccttgcgca ggatgtccag ggcgcggttc cggcacacgg
    11821 tgtaaagcca ggctttgagg ttgtcctgaa ctttggccgg ctcggtgagg tagagcttga
    11881 gcagggagtc ctgcaccacc tcgcgggcgc gctccacgtc gccgttgaga atgccagccg
    11941 agtaggcaat gagcgcgctc tcatgcaagt ccagcgcccg cctgacaagg agggaggctt
    12001 catctggatt cggctggacg ggcatggttt cactttgcag gactttcaac gcgcggggaa
    12061 gtcatttgtt agagtctgga gcctcgggag tggcgccggg acgcggggcg ttggcgggag
    12121 aaattgactt gcgccgccag cgcgccggaa cagcatggcg cgtgatgaaa actccccttc
    12181 tttccctgat cctcgccctg gccagcctgg ccggtctccg tgctcaaaat cttgaaacct
    12241 gggttctgac cgacgggcgc atgtttgagg cgcaggtcaa gcaggtggtg cccggcaccg
    12301 tcaccttcac cctgcgcacc ggcgccgacc agcctcttga aatcgccaag ctctccgagc
    12361 gcagcaaaaa acagctcgcc gaagttctcg gcctcggcca gtccgtggtg acacctctgt
    12421 cgcccgtggc ggccgccacc gctccagcgg cgacgccttc cgccactccg atgcccgcag
    12481 ccgcgc
//
LOCUS       DAOMYJ010000155        12443 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Prosthecobacter sp. isolate AMR_MDS_5629
            NODE_1700_length_12443_cov_21.286891, whole genome shotgun
            sequence.
ACCESSION   DAOMYJ010000155 DAOMYJ010000000
VERSION     DAOMYJ010000155.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867850
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prosthecobacter sp. (wastewater metagenome)
  ORGANISM  Prosthecobacter sp.
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Verrucomicrobiaceae; Prosthecobacter.
REFERENCE   1  (bases 1 to 12443)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12443)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.30
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:34:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,742
            CDSs (total)                      :: 4,684
            Genes (coding)                    :: 4,676
            CDSs (with protein)               :: 4,676
            Genes (RNA)                       :: 58
            tRNAs                             :: 55
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12443
                     /organism="Prosthecobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1700_length_12443_cov_21.286891"
                     /isolate="AMR_MDS_5629"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1965333"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            10..810
                     /gene="tpiA"
                     /locus_tag="PLP58_15115"
     CDS             10..810
                     /gene="tpiA"
                     /locus_tag="PLP58_15115"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007415152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HRJ09906.1"
                     /translation="MIPPAPRKPIIAANWKMHMTPQETDDFLRSFSRLVPDKCPVQIV
                     VAPPFVSLDRAQQTLMNAHEEVVELAAQNMSQHAGGAFTGEINARMIKECGCKHVILG
                     HSERRSIYGETSAVVNAKVLAALEARLHPILCIGETLEERDSGLIEKVLGGQLRESLA
                     EVGARRVQDCVIAYEPVWAIGTGRTASPQQAQEAHAYTRSVLGEMFGEDTAQKIRIQY
                     GGSVKPGNMAELIAQKDVDGALVGGASLESGSFWEICRAAIDHVNGKL"
     gene            complement(1104..1643)
                     /locus_tag="PLP58_15120"
     CDS             complement(1104..1643)
                     /locus_tag="PLP58_15120"
                     /inference="COORDINATES: protein motif:HMM:NF025390.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4240 domain-containing protein"
                     /protein_id="HRJ09907.1"
                     /translation="MNETDLHELLQSAWSESQGDWDMHEQVLHRKLMVLPLADLVDFD
                     VIQTNRRRLAFTPLLINAAFMVWDGALGNDGFLDFTDNLSVIPNDLFEAIIADADRVI
                     EVPDLWTPHEGHFWHLPFKVFDEQSRVKAGTDLLNEYLPPDNRPGDLWSRLDGHPNRQ
                     TAQNMLPRLYARFGESAFA"
     gene            complement(1667..3307)
                     /locus_tag="PLP58_15125"
     CDS             complement(1667..3307)
                     /locus_tag="PLP58_15125"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012421166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004333 - fumarate hydratase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate hydratase"
                     /protein_id="HRJ09908.1"
                     /translation="MPTPAFHYQPLLELGTDDTEYRLLTKDHVRVEKLGDKEMLVVDP
                     EALTLLANQAFHDINFFLRPKHLKQVAAILDDKEASENDRMVALTMLKNADVASAGLL
                     PFCQDTGTAIIMGKKGQQVWTGGGDEAALSKGVYLAYTENNLRYSQNAALNMFDEKNT
                     GTNLPAQLDLYATDGDAYKFLFIAKGGGSANKAFLYQETRAVLNPKSIVKFLTDKMVT
                     LGTAACPPYHLTFVIGGTSAESTMKHVKLASTKYYDNLPTTGNEHGRAFRDVELEAQM
                     LQAARECGIGAQFGGKYFALDVRIIRLPRHGASLPIGIGVSCSADRQAKGKITRDGVF
                     IEKLETNPLQYIPEELRHRKDTNAVRIDTNRPMAEIRAELSKYPTTTRLLINGPIIVA
                     RDIAHAKLKEMLDAGKPLPDYFKNHPVYYAGPAKTPAGMPSGSFGPTTAGRMDSYVDL
                     FQSHGGSMIMIAKGNRGQQVTDACKKHGGFYLGSIGGPAAILAKENIKKVEVVEFPEL
                     GMEAIWKIEVEDFPAFILIDDKGNDFFQSVGPGCSVKH"
     gene            complement(3356..4423)
                     /locus_tag="PLP58_15130"
     CDS             complement(3356..4423)
                     /locus_tag="PLP58_15130"
                     /inference="COORDINATES: protein motif:HMM:NF016159.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009295 - nucleoid [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein"
                     /protein_id="HRJ09909.1"
                     /translation="MPVKLRLNTATATRIVLAKVGNPQRQEPLQTSRQVFDIAEADQE
                     VLTALFLKPFKNLIAHRFVHHASLDHHEMHRCSRALFADPSRMLETGVEIARRLHAKS
                     NHPNIKSGDLCIVFIKEIHIEGELVNGLCILKSESVVPFLSITTQDGDLHLSTQQGIQ
                     PEKIDKGCLILDYQGAKGGYVLTFDRSGEDTKFWVREFLGVEAVPDSAFLTNAYAKMA
                     VSFIEEHKPAGDTPPWDTCNAAREALAYFEEREQFDLGEFEQEVLKSPDAVAAFEDHR
                     ARVVEEQGQPLDTRFEISKKDITKAKRRIGAVLKLDTGVEIHVKSTLSTEHDPVLERG
                     YDDAKGMKFVKVYYKEQLTAG"
     gene            4560..5210
                     /locus_tag="PLP58_15135"
     CDS             4560..5210
                     /locus_tag="PLP58_15135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009965971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease proteolytic subunit"
                     /protein_id="HRJ09910.1"
                     /translation="MSTPPHDIPSLITPPSNYIVPTVIETEGRMERAYDIYSRLLKDR
                     IIFLGTEVNDQVANIIIAQLLFLQMQDPKKDIHLYINSPGGSVTAGLAIYDTMQFVTC
                     DVNTYCIGIAASMGAVLLSAGTKGKRYALPNSDIMIHQVSGGARGTASDVERTVDFMY
                     KLKRRLNRILAHHTGKSPEIIEKDADRDNYMTAEEAAAYGLVDKVLQSNKELNPPQ"
     gene            5236..6510
                     /gene="clpX"
                     /locus_tag="PLP58_15140"
     CDS             5236..6510
                     /gene="clpX"
                     /locus_tag="PLP58_15140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009958715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HRJ09911.1"
                     /translation="MARASTVTLCSFCGKSHAEVRKLIAGPGVYICDNCINVCKGILD
                     REAVQEAEADQGLLVPKPAEIAAELDKHVIGQTHAKKVLSVAVHNHYKRILHLQQQSG
                     NQGGGARAVTDMNDVEIEKSNVLLLGPTGCGKTLLAKTLAKILNVPFSIADATTLTEA
                     GYVGEDVENIILRLLQAADYDIARAEIGIVYIDEIDKIGRTTGNVSITRDVSGEGVQQ
                     ALLKIVEGTICNVPPQGGRKHPQQEYIQVNTENILFICGGAFVGLDEIVKRRTGKQTL
                     GFNIAGKTDEAAPAKLEAEPEDLLAYGLIPEFIGRLPVACSLEPLTEVELVRVLTEPK
                     NALVKQYAKLMRMNGVDLNVNKEGLAAMAREAIKRGTGARGLRSIFERIMLDVMYDVP
                     SRADVRGVSINEAVVKGERPPILRKRVERNAA"
     gene            complement(6584..7771)
                     /gene="tatC"
                     /locus_tag="PLP58_15145"
     CDS             complement(6584..7771)
                     /gene="tatC"
                     /locus_tag="PLP58_15145"
                     /inference="COORDINATES: protein motif:HMM:TIGR00945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatC"
                     /protein_id="HRJ09912.1"
                     /translation="MWQGILNKVFKVREKVALNLGKEDEEKPFLEHLDDLRDMFVRIG
                     VTLIIAVIGMFFFYQELFRAIRYPMILAGLADDQAGAEALLQNTDVAGPFMMAINVTL
                     IAGVIISFPLLLIFVLQFILPGLRANEKKVLWPAIGIGTGLFLTGCTFAYWVVLPRAL
                     EFFDDFAATLGSRQDWTLSNYVTFTTRFILVFGIAFELPVIVMALVKLDFLNYKLMKS
                     TWRHALIAITLFAAIITPTPDVLTLMLMSGPLYFLYGICIVLAYFMEKKDREAYPEYY
                     AQIEKDEKELEEEVKDDWDNENYNPWFSEDKDDEEDEYHKPRPSAPPPEVEKAPNTVP
                     MSTDPAPAPPDDEEPDHEDQGSSVPDEEDEPLVTGRGPETPREEKTTEELAREDEQRG
                     GNP"
     gene            complement(7804..8427)
                     /locus_tag="PLP58_15150"
     CDS             complement(7804..8427)
                     /locus_tag="PLP58_15150"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HRJ09913.1"
                     /translation="MAGDGSRLEFPLFRVEDHDGWELVSSRRLFENPHVSVDENRYLT
                     PHRRQEPVPWLVVERKAAVAIAPVTADGEFVLISQERLPVRQTLWEFPAGQIDVPLAE
                     ITPEIVVETALRELREECGGVLAEGGRLEPLGWFLPSQGFTDEHVYLFRAEPVCVASR
                     PEPDGSEHISDVRLASPGELRRMIAANEITTALTLALFARMAAKGTL"
     gene            complement(8427..9791)
                     /gene="argH"
                     /locus_tag="PLP58_15155"
     CDS             complement(8427..9791)
                     /gene="argH"
                     /locus_tag="PLP58_15155"
                     /EC_number="4.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009964172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004056 - argininosuccinate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HRJ09914.1"
                     /translation="MWKGRFAQETSRLVQAYGESVSFDWRLFAHDIRGSIAHSKGLLK
                     AGILTAEEQAEIERGLLEIQAEIEHGDFEFKEALEDVHMNIEAELTRRIGPAGAKLHT
                     ARSRNDQVATDVRLYTRDAVDGLTALIRDLQRALIEAAERAGQAVVPGYTHLQRGQPV
                     LFAHHLLAYVEMLERDAGRLADARRRMDVMPLGSGALAGSTILLDRDHVAAQLGFAAV
                     SQNSMDAVSDRDFAVEVVSAIALCGVHLSRLSEDIILWCSAEFGFVTLSDAHTTGSSL
                     MPQKKNPDVAELTRGKSGRLVGNLVALLTLLKGLPMTYNRDMQEDKEPLFDSLDTLKA
                     ALEVFAEMIAGMEVNAARTLAATSDPMLLATDLADYLVKRGVPFRQAHDVIGKLVAHS
                     IAERAPFAEIPLAKYQEFSPAFAADLFDCLNLGTALAARQAVGAPSPQNVAAQIARWK
                     EALS"
     gene            9928..11763
                     /locus_tag="PLP58_15160"
     CDS             9928..11763
                     /locus_tag="PLP58_15160"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF012318.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like serine protease"
                     /protein_id="HRJ09915.1"
                     /translation="MKKSLFLMAGMLVMSCLVAQAQQKRIVGGINTTSGEYPFMTALV
                     EKGATPSSGQFCGAALVAPQWVLTAAHCLEGTPASTLDVWVGGHDLRTASQGVRVAVS
                     QVIMHPNYRETSQGALVNDFCLLKLASPVTTRATLPLVETSDQIAVGTMTRVLGWGAT
                     REGGAGSKILRQADVPLVSLTLAGVTNPGLNSTHLAAGFATGGVDSCQGDSGGPLMVP
                     GFSGQWLHAGTVSYGNGCARPNEYGIYGNTLTLKPWIIGHIGDPGEPPKPDPVDDHGN
                     TTAAATTLAVNGTLPGVLEAGGDLDVFKISALGAGVLTVTSGGGTDVTGTLLGSTGAA
                     LASDDNGNGAPNFRLTRTLTAAGTYYVRVAGVNPSTSGAYSITSAFTTTTGGDIALKT
                     GTTALPMNATLNFGALQVNAATVERTLTLTNTGTTTLTIVGASFDGADAGSFHFVSLP
                     AASLAKGKSTSFIISCAPDRAGALSARLLVQSSAAGKNPYPVNLVASATATTPDDHGN
                     NFYFATAAPVPGGIPGVINHGADKDFFVITVAVKTTVTMKTTGFVDTYGTLYSSAGKV
                     LKKADDTGRDLNFTLKRALTPGTYYLCVEGYDRHATGPYTLLLSR"
     gene            11785..>12443
                     /locus_tag="PLP58_15165"
     CDS             11785..>12443
                     /locus_tag="PLP58_15165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ09916.1"
                     /translation="MKTALSLTLAALLLLAVLAGIRLAGPGARPLKKEIETVATAPVR
                     PPPQAPGLPPAQVVEVPLQVLDAATAPPRDPVAAILERWSRARTPQEHDEHAEALAAL
                     GTVEAVQALMEKLARVRDAEARGLIAANLRAIAEPDTLAALVPALLGDFRQGSPELRE
                     LCSAIGRLAQAETVQALEDLHWHAASLAGQSPKVVRAMASIRNPAAARGNFQQAALDL
                     V"
BASE COUNT         2303 a         3911 c         3930 g         2299 t
ORIGIN      
        1 agcgagccca tgatcccgcc cgccccccgc aaacccatca tcgccgccaa ctggaagatg
       61 cacatgaccc cgcaggagac cgacgacttc ctgcgctcct tctcccgcct cgtgccggac
      121 aaatgccccg tgcagatcgt cgtcgccccg cccttcgtca gcctcgatcg cgcgcagcag
      181 accctgatga acgcccatga ggaagtcgtc gaactggccg cgcagaacat gagccagcac
      241 gccggcggcg ccttcaccgg cgagatcaac gcgcgcatga tcaaggaatg cggctgcaag
      301 cacgtcatcc tcggtcacag tgagcgtcgc agcatctacg gcgagaccag cgccgttgtg
      361 aacgccaagg tgctcgccgc gctcgaggcg cgcctgcacc ccatcctctg catcggcgag
      421 acgctggagg agcgcgattc cggcctcatt gaaaaagtgc ttggcggaca gctccgcgaa
      481 tcgctcgccg aagtcggcgc ccgccgcgtg caggattgtg tcatcgccta cgagcccgtc
      541 tgggccatcg gaaccggacg caccgccagt ccgcagcagg cgcaggaggc ccacgcctac
      601 acccgctccg tgctgggcga gatgtttggc gaggacaccg cgcagaaaat ccgcatccaa
      661 tacggcggca gcgtgaagcc gggcaacatg gccgaactca tcgcccagaa agacgtggac
      721 ggcgcgctcg tcggcggcgc cagcctggag tccggcagct tctgggagat ctgccgcgcc
      781 gccatagacc atgtgaacgg caagctctag aactttgacg gcagacactt cctgccaatg
      841 attgcgggcc gacgaagtgt ggctgcgcga cagctcctcc aagccagatt tgaaaccgcc
      901 ccgcgccctc aaagggtgcg agaagggcat tcggagtctg acgggcaaaa tcgtcggagg
      961 agcttcgtca tcttctcgcg cccctgccgg ggcgcatccc gtatcggagg tggtggggcg
     1021 gtccggttcc ggtggttccc ggtcgctccg cgaccttcac caccggctaa tctctttcga
     1081 gcctccggct cggggatacc ctcctaagcg aaagctgatt cgccaaatcg tgcataaagg
     1141 cgaggaagca tgttttgggc ggtttgacgg ttcggatgtc catctagccg tgaccataga
     1201 tcaccaggac ggttgtccgg cggcagatac tcattcagca aatcagttcc ggcttttaca
     1261 cgactttgtt catcgaagac tttgaatgga aggtgccaaa aatgcccttc gtggggtgtc
     1321 cacaggtccg gaacttcaat cactctgtct gcatcagcta tgatcgcctc aaacagatcg
     1381 tttggaatga cggataggtt atctgtgaaa tccaagaagc catcattccc tagcgcccca
     1441 tcccaaacca tgaatgcggc gttgatcaac aagggagtga atgcaagccg gcgtcggttg
     1501 gtttggatga cgtcgaaatc aacaaggtcg gcgagcggca ggaccatcag ttttcgatga
     1561 agaacttgct cgtgcatgtc ccaatcgccc tgcgactccg accatgcaga ttggagcagt
     1621 tcatgaaggt ctgtctcatt catggccgaa attgaaaggc actgcctcaa tgcttcaccg
     1681 agcaccccgg tccgacgctt tggaaaaagt cgttcccctt gtcgtcgatg aggatgaagg
     1741 cggggaagtc ttcgacttcg atcttccaga tggcttccat gccgagctcg gggaactcga
     1801 cgacttcgac cttcttgatg ttttccttgg cgaggatcgc ggcggggccg ccgatgctgc
     1861 cgaggtagaa gccgccgtgc tttttacatg cgtcggtgac ctgctggccg cgattgcctt
     1921 tggcgatcat gatcatgctg ccgccgtggc tctggaagag atcgacgtag ctgtccatgc
     1981 ggccggcggt ggtggggccg aagctgccgc tgggcatgcc ggcgggtgtt ttggcgggac
     2041 cggcgtaata gacggggtgg tttttgaagt aatccggcag cggcttgccg gcatcgagca
     2101 tctccttcag cttcgcatgc gcgatgtcgc gggcgacgat gatggggccg ttgatgagga
     2161 ggcgcgtggt ggtgggatac ttgctcagct cggcgcggat ctcggccatc gggcgattcg
     2221 tgtcgatgcg cacggcgttg gtgtctttgc ggtggcgcag ctcctcgggg atgtattgca
     2281 gcgggttcgt ttcgagtttt tcgatgaaga cgccgtcacg cgtgatcttg cccttcgcct
     2341 ggcggtcagc ggagcaggaa acgccgatgc cgatgggcaa agacgcgccg tgacgcggca
     2401 ggcggatgat gcgcacatcg agtgcgaagt atttgccgcc aaactgcgcc ccgatgccgc
     2461 actcgcgagc ggcctggagc atttgagctt cgagttcgac atcgcggaag gcgcggccgt
     2521 gctcgttgcc ggtggtgggg aggttgtcgt aatacttcgt ggaggcgagt ttgacgtgtt
     2581 tcatcgtcga ttcggccgag gtgccgccga taacgaaggt gaggtggtaa ggcgggcacg
     2641 cggcggtgcc gagcgtgacc attttgtcgg tcaaaaactt cacgatgctc ttcggattga
     2701 gcacggcgcg ggtttcctga tacaggaagg ccttgttagc cgagccgccg cctttggcga
     2761 tgaagaggaa cttatacgcg tcgccatcgg tggcatacaa atcgagctgc gcgggcagat
     2821 tggtgccggt gttcttttcg tcgaacatgt tcagcgcggc gttttgcgag tagcgcaggt
     2881 tgttctccgt gtaggcgaga tagacgccct tcgagagcgc ggcctcatca ccgccgccgg
     2941 tccagacctg ctggcctttt ttgcccatga tgatggcggt gccggtgtcc tggcagaagg
     3001 gcagcaggcc cgcggaggcc acgtcggcat tcttgagcat cgtgagcgcc accatgcggt
     3061 cgttttcgct cgcttccttg tcatcgagga tggcggcgac ctgcttcagg tgcttcggcc
     3121 gcaggaagaa attgatgtca tgaaacgcct ggttcgccag cagcgtgagc gcctcgggat
     3181 cgacgacgag catctcttta tcgcccagtt tttcgacgcg gacgtgatcc ttcgtcagca
     3241 ggcggtattc agtgtcgtcg gtgccgagtt cgaggagagg ctggtaatgg aaagcaggcg
     3301 tgggcatgat gagggattgt gaaatggata cgccgcgcgg caacgggaaa gcggctcagc
     3361 ccgctgtgag ctgctctttg tagtagacct tcacgaactt catccctttg gcgtcgtcat
     3421 agccgcgttc caggacgggg tcgtgctcgg tggagagggt ggatttgaca tggatttcga
     3481 cgccggtgtc gagcttgagc acagcgccga tgcgcctttt ggccttggtg atgtccttct
     3541 tggaaatctc gaacctggtg tcgagcggct ggccttgctc ttcgacgacg cgggcgcggt
     3601 ggtcttcgaa ggcggcgacg gcatcgggtg acttgagtac ctcctgctcg aactcaccga
     3661 ggtcaaactg ctcgcgctcc tcaaagtagg ccagggcttc gcgggcggcg ttgcaggtgt
     3721 cccagggcgg cgtgtccccg gcgggtttgt gctcctcgat gaaggagacg gccatttttg
     3781 cataggcgtt ggtgaggaag gcggagtccg gcacggcctc gacgccgagg aactcgcgca
     3841 cccagaattt ggtgtcctcg ccgctgcggt caaaggtgag cacatagccg cctttggcgc
     3901 cctgatagtc gaggatgagg cagcctttgt ctatcttctc cggctggatg ccctgctgcg
     3961 tggagaggtg gaggtcgcca tcctgcgtgg tgatgctgag gaagggcacg acgctctcgg
     4021 acttgaggat gcacaagccg ttgaccagct caccctcgat gtggatttcc ttgatgaaga
     4081 cgatgcaaag atcgccggac ttgatgttgg gatgattcga cttggcgtgc agacgcctgg
     4141 cgatctcgac gccggtctcg agcatcctgg acggatcggc gaacaacgcc ctggagcagc
     4201 ggtgcatctc gtggtgatcc agcgaggcgt ggtggacgaa acggtgggcg atgaggttct
     4261 tgaagggttt caaaaacagc gcggtgagca cctcctggtc cgcctcggcg atgtcgaaga
     4321 cctggcggga cgtctggaga ggttcctggc gctgcgggtt gccgaccttg gcgaggacga
     4381 tccgcgtggc ggtggcggtg ttgaggcgga gtttgacggg catggcgggg aaagggcgcg
     4441 gagaataaaa gacaatcccg ccgcggaaag cgaaaccctt ctccaaagcg aaaggttttt
     4501 ctttggagcc gggaaggagc cttgtttgac aatccatcac gccgccgttt gattgccgca
     4561 tgtccacccc cccccacgac atcccgtccc tcatcacgcc gccctccaac tacatcgtgc
     4621 ccaccgtcat cgagactgag gggcgcatgg agcgggcgta cgacatctac tcgcggcttt
     4681 tgaaggaccg catcatcttt ctaggcacgg aggtgaacga ccaggtggcg aacatcatca
     4741 tcgcccagct tttgttcctc cagatgcagg atccgaagaa ggacattcac ctctacatca
     4801 acagccccgg cggctccgtg accgccggcc tcgccattta tgacaccatg cagttcgtca
     4861 cctgcgacgt gaacacctac tgcatcggca tcgccgcgag catgggcgct gtgctgctct
     4921 ccgcgggcac caagggcaag cgctacgcgc ttccaaacag cgacatcatg atccaccagg
     4981 tgtccggcgg agcgcgcggc acggccagcg atgtcgagcg caccgtggac ttcatgtaca
     5041 agctcaagcg ccgcctgaac cgcatcctgg cgcaccacac cggcaagagc cccgaaatca
     5101 tcgaaaagga cgccgaccgc gacaactaca tgaccgccga ggaggccgcc gcctatggcc
     5161 tggtggacaa ggtgctgcaa agcaacaagg aactcaaccc gccgcagtaa ccgcggcgca
     5221 aatcccctcc gccgcatggc ccgcgcctcc accgtcaccc tctgctcctt ctgcggcaaa
     5281 agccacgcgg aggtcaggaa gctcatcgcc gggccgggcg tgtatatttg cgacaattgc
     5341 atcaacgtct gcaagggcat ccttgatcgc gaggccgtgc aggaggcgga agcggaccag
     5401 gggctgctgg tgccgaagcc ggcggagatc gcggccgagc tggacaagca tgtcatcggc
     5461 cagacgcacg ccaagaaggt gctcagcgtc gccgtccaca atcattacaa gcgcatcctg
     5521 catctgcagc agcagtctgg aaaccagggc gggggagcca gggcggtgac tgacatgaac
     5581 gatgtcgaga tcgagaaaag caacgttctc ctgctcggcc ccaccggctg cggcaagacc
     5641 ttgctggcca agacccttgc gaaaattctg aacgtgccct tcagcatcgc ggacgccacc
     5701 accctcactg aggccggata tgtgggagag gatgtggaaa acatcatcct gcgcctgctt
     5761 caggctgcgg attatgacat cgccagggct gagatcggca tcgtttacat agacgagata
     5821 gacaagatcg gccgcaccac gggcaacgtc agcatcacgc gtgacgtgtc cggtgagggt
     5881 gtgcagcagg cgcttttaaa gattgtcgag ggcaccattt gcaatgtgcc gccgcagggc
     5941 gggcggaagc atccgcagca ggagtacatc caggtcaaca ctgagaacat cctgttcatc
     6001 tgcggcggcg cgtttgttgg actcgatgag atcgtcaagc gccgcacggg caagcagaca
     6061 cttggattca atatcgctgg aaaaacggac gaggcggccc ccgccaagct ggaggcggag
     6121 cctgaggatc tgctcgccta cggtctcatc cccgaattca tcgggcgttt gcccgtcgcc
     6181 tgctccctgg agccgctcac cgaggtggaa ctcgtgcgcg tcctgacgga gccaaagaac
     6241 gccctggtca agcagtacgc caagctcatg cgcatgaacg gcgtggacct caatgtgaac
     6301 aaggaagggt tggcggccat ggcgcgcgag gccatcaaac gcggcaccgg cgcgcgcgga
     6361 ctgcgcagca tctttgagcg aatcatgctt gatgtgatgt atgacgtgcc cagccgcgcc
     6421 gatgtgcgcg gcgtctccat caacgaggcc gtggtcaaag gcgagcgtcc gcccattctg
     6481 cgcaagcggg tcgagcgcaa cgccgcgtga catgcggcgg ttttgagggt aaacctggtc
     6541 gcttccgccc tggcgtgagg atcgtccagg gtgagcgtgg tatttatgga ttgccgccgc
     6601 gctgctcgtc ctcgcgggcg agttcctcgg tggtcttttc ctcgcgcggg gtttcggggc
     6661 cgcgtcctgt gacaaggggc tcgtcctcct cgtcgggcac ggaggagcct tggtcctcgt
     6721 gatcgggctc ctcgtcatca ggcggcgcgg gcgctgggtc ggtgctcatg ggcacggtgt
     6781 tcggcgcttt ctccacctcg ggcggcggcg cggaggggcg gggcttgtgg tattcgtcct
     6841 cctcgtcatc cttgtcctcg gagaaccagg ggttgtagtt ctcgttgtcc cagtcgtcct
     6901 tcacctcctc ttcgagctct ttttcatcct tctcgatctg ggcgtaatac tcggggtagg
     6961 cctcgcggtc cttcttctcc atgaagtagg ccagcacgat gcagatgccg tagaggaaat
     7021 aaagcgggcc ggacatcagc atgagcgtga gcacatccgg cgtcggcgtg atgatggccg
     7081 cgaagagcgt gatggcgatc aaagcgtgcc gccaggtgga cttcatgagc ttgtagttca
     7141 ggaagtccag cttcacgagg gccatgacaa tgaccggcag ctcgaaggcg atgccgaaga
     7201 cgagaatgaa gcgggtggtg aaggtgacgt agttggaaag cgtccagtcc tgccgcgaac
     7261 cgagtgtggc ggcgaagtcg tcgaaaaact ccaaggctct cggcagcacg acccaatagg
     7321 caaaggtgca gccagtcaga aaaaggccgg tgccgatgcc gatggccggc catagcacct
     7381 tcttttcatt cgccctcaat cccggcagga tgaactggag cacaaagatg agcaggaggg
     7441 ggaaggaaat gatgactccc gcgatcagcg tgacattgat cgccatcatg aaggggcctg
     7501 cgacatcggt gttttgcagc agcgcctccg cgcccgcctg gtcgtccgcc agcccggcga
     7561 ggatcatggg atagcggatg gcgcggaaca actcctgata aaaaaagaac atgccgatca
     7621 cggcgatgat cagcgtcacg ccaatgcgca caaacatgtc gcgcaggtcg tcgaggtgct
     7681 ccaggaaggg cttctcctca tcctccttgc cgaggttgag cgccactttt tcgcggactt
     7741 tgaagacttt gttcaggatg ccttgccaca tggttcaaat cgaggggtga atgggtttgc
     7801 gcctcaaaga gttcccttcg ccgccatccg cgcgaacaaa gccagtgtca gggcggtggt
     7861 gatctcattg gcagcgatca tcctccgcag ttctcccgga ctggccaggc gcacgtcgct
     7921 gatgtgctcg cttccatccg gctcgggacg gctggcgaca cacacgggct ccgctctgaa
     7981 aagataaacg tgctcgtcgg tgaatccttg cgagggaagg aaccagccca gcggctccag
     8041 gcgccctccc tcggctagca cgccgccgca ttcctcgcgc aactcgcgca gggcggtctc
     8101 gacgacgatc tccggcgtga tctccgccag cggcacgtct atctggccgg cggggaattc
     8161 ccataaagtc tgccgcaccg gcaggcgctc ctgggaaatg agcacaaact ctccgtccgc
     8221 cgtgaccggc gcgatggcca cggcggcctt cctttccacc accagccagg gcacgggctc
     8281 ctgcctgcgg tgcggggtga ggtagcggtt ctcatccacg ctgacgtgcg gattttcaaa
     8341 aagccggcgg ctggagacca gctcccagcc gtcgtgatcc tcgacacgga acaaggggaa
     8401 ttcgagtctc gatccatccc ccgccatcac gagagcgcct ccttccagcg ggcgatctgc
     8461 gccgccacat tttgcggcga gggcgcgccc acggcctggc gcgcggcgag cgcggttccg
     8521 agattcaggc agtcaaagag gtccgcggca aaggcggggg agaactcctg gtacttcgcg
     8581 agtggtattt cggcaaaagg cgccctctcc gcgatggagt gcgccaccag tttgccgatg
     8641 acatcatgcg cctggcggaa gggcacgccg cgcttcacca ggtagtccgc caggtccgtg
     8701 gcgagcagca tgggatcact cgtggcggcg agtgtgcgcg ccgcgttcac ctccatgccg
     8761 gcgatcattt ccgcgaagac ctccaatgcc gccttcaagg tgtcgagcga gtcgaagagc
     8821 ggctccttgt cctcctgcat gtcgcggttg taggtcatcg gcaggccttt gagcagggtc
     8881 aaaagcgcga cgagattgcc cacgagacgt ccggattttc cgcgcgtcag ctcggccaca
     8941 tccgggttct ttttctgcgg catcagggag gagccggtgg tgtgcgcgtc gctcagcgtc
     9001 acaaagccaa actcggcgct gcaccagaga atgatgtcct cgctcaagcg cgagagatgc
     9061 accccgcaga gggcgatggc ggagaccacc tccacggcga agtcgcggtc gctcaccgcg
     9121 tccatgctgt tttgggaaac ggcggcaaag ccgagctgcg cggccacatg atcgcggtcg
     9181 agcaggatgg tggagccggc cagggcgccg gagccgagcg gcatcacatc catgcggcgg
     9241 cgcgcgtcgg ccaggcggcc cgcgtcgcgc tccagcatct ccacataggc cagcagatgg
     9301 tgggcgaaaa gcacgggctg cccgcgctgg agatgcgtgt aaccgggcac cacggcctgc
     9361 ccggcgcgct cggcggcctc gatgagcgcg cgctgcagat cacggatcag cgccgtcagg
     9421 ccgtccaccg cgtcgcgcgt gtagaggcgc acatccgtgg ccacctggtc gttgcggctg
     9481 cgcgcggtgt gcagcttcgc cccggcgggg ccgatgcggc gcgtcagctc cgcctcgatg
     9541 ttcatgtgga cgtcctccag tgcctctttg aactcaaagt ccccgtgctc gatctccgcc
     9601 tggatttcca aaaggccgcg ctcaatctcc gcctgctcct ccgccgtcag gatgcccgcc
     9661 ttcagcagcc ccttcgagtg ggcgatcgag ccccggatgt catgcgcgaa gagcctccag
     9721 tcaaacgaca cggactctcc gtaagcctgc acaaggcggc tggtttcctg ggcaaaacga
     9781 cctttccaca taaaacgggc gctgagccta gcggcattcg tcctggatgc aaccctgccc
     9841 tgggacaaga atctccttgc ttcgagcggt ctgggtggga gctttccgcg tccgtctcat
     9901 agcaacgctc aacaaccaag ccggcccatg aaaaaatccc tgttcttaat ggcgggaatg
     9961 ctcgtcatgt cctgcctcgt cgcgcaggcc cagcagaaac gcattgtcgg tggcatcaac
    10021 accacttcgg gggagtaccc cttcatgaca gcactcgttg aaaaaggagc cacaccgtcg
    10081 tcggggcagt tctgcggcgc ggctctcgtg gctccgcaat gggtgctgac tgcggcgcac
    10141 tgcctggaag gcacacccgc ctccactctg gatgtctggg tgggcgggca cgacctgcgc
    10201 accgcctccc agggcgtgcg tgtggccgtc tcccaggtca tcatgcatcc gaactaccgc
    10261 gagacctccc agggcgcgct ggtcaatgat ttctgcctgc tgaagctggc cagccccgtg
    10321 accacccgcg ccactctgcc cctggtggaa acctccgacc agatcgccgt cggcaccatg
    10381 acacgcgtgc tcggctgggg ggccacgcgc gagggcggcg cgggatcgaa aatcttgcgc
    10441 caggcggatg tgcccctggt ttccctgacg ctggcgggtg tcaccaatcc gggcctcaac
    10501 agcacgcatc tggccgccgg atttgccacc ggcggcgtgg actcctgcca gggggacagc
    10561 ggcggaccgc tcatggtgcc gggtttttca gggcagtggc tgcacgccgg aacggtgagc
    10621 tacggcaacg gctgcgcgcg gcccaacgag tatggcatct acggcaacac gctgactttg
    10681 aagccctgga tcatcggcca catcggcgac cccggcgagc cccccaagcc cgacccggtg
    10741 gacgaccacg gcaacaccac cgccgctgcc acgactctgg cggtcaatgg cacgctgccc
    10801 ggcgttcttg aggccggtgg cgaccttgat gtcttcaaaa taagcgccct tggcgcgggc
    10861 gtgcttaccg tcacctccgg cggcggcact gatgtcaccg gcacgcttct gggcagcacc
    10921 ggtgccgcgc tcgccagcga tgacaacggc aatggcgcgc ccaacttcag gctcacacgc
    10981 acccttaccg ccgcaggcac ctattatgtt cgcgtggccg gagtgaaccc gtcaacctcc
    11041 ggtgcctaca gcatcacctc cgccttcacc accacaaccg gcggagacat tgccctcaaa
    11101 accggcacca ccgccctgcc catgaacgcc accctgaact ttggcgcgct tcaggtcaat
    11161 gcggccacag ttgaaagaac tctcaccctt accaataccg gcaccaccac cctgacgatt
    11221 gtcggggcca gcttcgatgg cgcggacgcg ggcagttttc attttgtctc cctgccggcg
    11281 gccagcctcg ccaagggcaa gtccaccagc ttcatcatct catgcgcgcc ggaccgagcg
    11341 ggcgccctca gcgcgcgcct gctggtgcaa agcagtgccg cgggcaaaaa cccctatccg
    11401 gttaacctcg tcgcctctgc caccgccact actcccgacg accacggcaa caacttctac
    11461 ttcgccaccg ccgcgcccgt gccgggcggc attcccggcg tcatcaatca cggcgccgac
    11521 aaggatttct ttgtcatcac cgtggccgtg aagaccaccg tgacgatgaa gaccaccggc
    11581 tttgtggaca cctacggcac gctgtattcc tcagccggga aggtgctcaa aaaagcggat
    11641 gacaccggcc gcgacctgaa cttcaccctc aagcgcgcgc tgacgccggg cacttactac
    11701 ctgtgcgtgg aaggctacga ccgccatgcc accggcccgt acacgctcct gctttcccgc
    11761 tgaccccggg cacgggccgc gcgcatgaag accgccctca gcctcacttt ggcagcgctg
    11821 ctattgctcg ccgtactggc tggcattcgc ctcgctgggc cgggagcgcg gccgttgaaa
    11881 aaggaaatcg aaaccgttgc caccgctccg gtgcgtccgc cgccgcaggc tcccggtttg
    11941 ccgcctgctc aagtcgtcga ggtgccgctc caggtacttg acgccgcaac ggctccaccg
    12001 cgcgatcctg tggccgccat cctggagcga tggtcgcgcg cgcgcacgcc gcaggagcac
    12061 gatgagcacg ccgaggccct ggccgcgctc ggcaccgtgg aggccgtgca ggccctgatg
    12121 gaaaaactgg cccgggtgcg cgacgcggaa gcgcgcggac tcatcgccgc caacctgcgc
    12181 gccatcgccg agccggatac tctggccgcg cttgtgcctg ccctgctcgg agacttccgc
    12241 cagggcagcc ccgaactgcg cgagctgtgt tctgccattg gcaggctggc gcaggctgag
    12301 acggtgcagg ctctcgaaga cctgcactgg catgccgcca gcctcgcggg ccaaagcccg
    12361 aaagtcgtcc gcgccatggc ctccatccgc aaccccgccg ccgcgcgcgg caacttccag
    12421 caggcggcgc tggacctggt cta
//
LOCUS       DAOMYJ010000156        12432 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Prosthecobacter sp. isolate AMR_MDS_5629
            NODE_1703_length_12432_cov_18.178234, whole genome shotgun
            sequence.
ACCESSION   DAOMYJ010000156 DAOMYJ010000000
VERSION     DAOMYJ010000156.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867850
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prosthecobacter sp. (wastewater metagenome)
  ORGANISM  Prosthecobacter sp.
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Verrucomicrobiaceae; Prosthecobacter.
REFERENCE   1  (bases 1 to 12432)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12432)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.30
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:34:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,742
            CDSs (total)                      :: 4,684
            Genes (coding)                    :: 4,676
            CDSs (with protein)               :: 4,676
            Genes (RNA)                       :: 58
            tRNAs                             :: 55
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12432
                     /organism="Prosthecobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1703_length_12432_cov_18.178234"
                     /isolate="AMR_MDS_5629"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1965333"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            90..983
                     /locus_tag="PLP58_15170"
     CDS             90..983
                     /locus_tag="PLP58_15170"
                     /inference="COORDINATES: protein motif:HMM:NF018963.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1460 domain-containing protein"
                     /protein_id="HRJ09917.1"
                     /translation="MSLHPTRRRFLLALTACGVTDSVKGVEHLPQSVTFKGREKFDRI
                     VARGIAEQWSRLPMGARVMSAALAMEGTPYAGFTLEIDDHVESPSVNFDGLDCWTFFE
                     TALGLARMFGRSHGSRTPAGLLREIETTRYRGGLCRGGYLERIHYLEEWFRDNDRRGH
                     VRDITATLGPLTEIKGRRIDEMTVLWKSYRYLANNPDLRAGMARIEAELQSHPFHYLP
                     KAAVQGIEHKLESGDIVGIVTHKPHVYCSHAGLILRDAAGARRFMHASTTRKRVLVDK
                     PLHAYLADFKSHAGIIACRPL"
     gene            997..2229
                     /locus_tag="PLP58_15175"
     CDS             997..2229
                     /locus_tag="PLP58_15175"
                     /EC_number="2.4.2.4"
                     /inference="COORDINATES: protein motif:HMM:NF004490.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006206 - pyrimidine nucleobase metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine phosphorylase"
                     /protein_id="HRJ09918.1"
                     /translation="MHVPSLIEKKRDGQALAEEEIHALIRAFTLGELPDYQMSALAMA
                     VYFQGMTASETAALTHAMLHSGIVLSWPAGSPPRVDKHSTGGIGDKTSLVLAPLLACD
                     GLWVPMISGRGLGITGGTLDKLESIPGFRTRLEEKEIHRVVSDVGCVMVGQTEGLCPA
                     DKKLYALRDVTATVPSIPLITASIMGKKLAEGLDRLVLDVKHGTGAFMRTEEQARALA
                     DSMVAVGTALGVRCAVRINAMSEPTGRAAGNALEVAECVECLQGRGPGDLEDIVLELA
                     GAVSDTPRATHEQRLRDGSAWRKFQQMTAAQGGDISVLEKITEVHAAPVIIEVPAPAS
                     GIVRRMDAGTIGRAVLALGAGRSRASDPVDFAVGVDRIVKTGESVTAGAPLLRVHART
                     PAAAEAAMRTILEGVEIR"
     gene            2356..3288
                     /locus_tag="PLP58_15180"
     CDS             2356..3288
                     /locus_tag="PLP58_15180"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: protein motif:HMM:NF004160.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003919 - FMN adenylyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional riboflavin kinase/FAD synthetase"
                     /protein_id="HRJ09919.1"
                     /translation="MQKLESIPQLGCLRGPLALAIGVFDGLHLGHQEVILAAREHAAR
                     HEGRAVVLTFDPHPLRVLRPESAPLRLCGSRYRAVLLERMGLDAMLVCPFTPELASTE
                     AEDFVAELVGVCRPLGCVSVGYAWTFGKNRGGTIHSLMDLGQRHGFAVYGVPPVRVDG
                     EVVSSTLIRQAVAAGDLARAAALSGRPYALFGKVVEGRKLGRGLGFPTANVRVEAEML
                     PPYGVYAASADIGGRTLAGVANLGVRPTVEKTGSVPMLEVHFFDWQGDLYAQDLEVRL
                     GAFMRPERKFAGMAELRAQIASDVLLARSAPLPG"
     gene            complement(3268..4113)
                     /locus_tag="PLP58_15185"
     CDS             complement(3268..4113)
                     /locus_tag="PLP58_15185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ09920.1"
                     /translation="MTFCAYNLKNWLVMESPGGSKRPPQSKPDAEKQKVIEFLSEIQP
                     DILGVCEIGTREDLQELQSRLKDAGMDLPHSEFNQGADVTRSLALLSRFPISARHHQG
                     ALPYQIGGETFFFQRGILDATVDIRPDFQVRFLGVHLKSKREVAEGDQALMRRNEAHL
                     TRAHLDKIFSLQSRPNLVLYGDFNEYRNESSMDEIIGSRAQESYMLEIPVKDSHGLLW
                     THFWDVADVYSRLDYLFVSRNLRPLVDTKKSRIHTSADFDKASDHRPVVLNFEAPQAS
                     AGEGR"
     gene            complement(4220..4483)
                     /locus_tag="PLP58_15190"
     CDS             complement(4220..4483)
                     /locus_tag="PLP58_15190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009961081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase TatA/TatE family
                     subunit"
                     /protein_id="HRJ09921.1"
                     /translation="MNTPSILAAFGPLGTPELIIIAILVLVLFGAKKLPTFARSLGKS
                     MGEFKKARDEFEHELTRAQEEVEKPTIPATAEKRQPVGSSQDV"
     gene            4597..6774
                     /locus_tag="PLP58_15195"
     CDS             4597..6774
                     /locus_tag="PLP58_15195"
                     /inference="COORDINATES: protein motif:HMM:NF012869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRJ09922.1"
                     /translation="MAKPPDAASPNSPDSSALHEHDVQTLAHLCGLTGTPFDRMRASQ
                     GLHNAARSCKEPLERLAAAASECGISITPARLTLADAIWRAADSVPFAAWSAKSQSWL
                     VIHKHGFFKARVSDPENPSETENLTRGELASRLGLASAEAPADFGIVSAELPMRALGQ
                     DAHHGHAAVSPVRRFIALMRPERKDIGSVIIFSLVTGVLYLALPLAVNALVSNLAFGG
                     QSGPLWTALLIMAFALTLALLLSAFIRAMQYYIVEIIQRRLFVRLAADLSHRLPRVCA
                     SSLDGVHAPELVNRFLDVVTVQKSTALMLLDGINIVLGMVVGMTVLGLYHPYLLGFVI
                     LLLALLLGTMLVLGRGAVRTSVGESLAKYEVVGWLEELARYPRLFKGPGGYRIAAERA
                     DDLTRRYLDNRRAHFRVLYRQIAGLLVMEVVASAALLIVGGWLVISQELTLGQLVASE
                     LIVGTIVASLSKLGKKFEAWYDAMAAMDKLGHLVDLPVEREDGEFPPRKPDGARLEVG
                     TLGYTYPYGVPVFTGLSFEVAPGERVALTGTQGSGSSTLLDILHGGREPSEGYIRLDG
                     LDLRSWYLEALREQVALVRPDDIFQGSVVENTRLGNMSITMDEVTAALDRVGLLRELL
                     EMPDGVNTQLITGGLPLSSRQRIRLVLARALVTRPRLLLLDEIFDGLDDASMDELCGI
                     ILDEKNPWTVLVATRDAAVLRRCQRVLDLSRFAEAAHPPTQAR"
     gene            6771..8060
                     /locus_tag="PLP58_15200"
     CDS             6771..8060
                     /locus_tag="PLP58_15200"
                     /inference="COORDINATES: protein motif:HMM:NF024829.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family efflux transporter periplasmic
                     adaptor subunit"
                     /protein_id="HRJ09923.1"
                     /translation="MKAHAIPTLELRGRLPCMALAGSSRFSRILARVLAILFVLSLIG
                     VFVLPWQQNVPGAGRVIAFDPLERRINVEAPVSGRVKKLHVVEGQRVKKGDILVEIQD
                     NDPNLLANLRLQRDAAIARRAAAKQRVEDLTQQAQEMELAKSQAIDAARQRIAAEKIT
                     AETAVLNHQRTVTLAEKGLVSTRDLELATLARDSSAANLAAAEATLTRTEREMEASIS
                     NIRANRGTAQGDVAAAEREMASIDSLISQNLQQVIESPRDGIVLSVTATDGTFLRPGS
                     PICVVIPETESRFVELMLDGNDIPLVTARQVHEDGTITEGSPVRLQFEGWPAVQFIGW
                     PSVAVGTFGGEVVFVDATDDGMGQFRVVVAPKPDVIVRRGVERVEPWPGNRWLRQGVR
                     AKGWVLLQQVPLWKEIWRQLNGFPPVISNTEPVEKKT"
     gene            8485..9903
                     /locus_tag="PLP58_15205"
     CDS             8485..9903
                     /locus_tag="PLP58_15205"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HRJ09924.1"
                     /translation="MNRYRALRSSLLALLLAATTPAAAEKPLTLDEVLASVKSTYPPY
                     LAALIEMDIANGRVRQAAGAFDLNLSAGGSFTPSGYYDGRSGAALLEQPLPFWGGSLF
                     GGYQLSSGFLPNYNKDRTQRDGEAILGFKLNLLRDGTLDRRRAQLYQARVDQELADPL
                     ILRQYIDFIRAASIAYYNWLAAGHRWQLAEELLRVAKDRDEAIAEQAGRGAVAPIICV
                     DNERLVVSRQISVVQARRRFEAAAIELSLFHRGEDDEPVISPRSRLLSAFPEPPVPDA
                     TRLSSDIAKALIFRPEIRRLDLTLEKADIERRLAKNNLLPNLDLTLQANQAVSERTQK
                     DMERLEMEAKIQFSLPLQRNEAKGRLETIEAQMSRLQTERQFARDRISADVRDAMSAV
                     QAARQQITQTRRNVELATQIEQAEALKFQQGAADLLALQIREQATFDARQLDVEAHAE
                     YLRALANHRAAIATDAPASLAR"
     gene            10031..>12432
                     /locus_tag="PLP58_15210"
     CDS             10031..>12432
                     /locus_tag="PLP58_15210"
                     /inference="COORDINATES: protein motif:HMM:NF012321.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HRJ09925.1"
                     /translation="MDWESPKLLLLILPALLLLLWIEGASAHPMTGLRKRLLLVARAL
                     GVMLALAALAGPARVSESGRKSLGIVVDASQSLGAEGYARALAEAERVRAGAGVETFV
                     VRFGSRPELLPAEAAETPEQAAAWREKNGGDSHYAAALELARALFPPGASRTLLLIGD
                     GHETRGSLLDTARDAALAGVRLHTLPVAGTLRPDARVLSLTPSRPRLNEGATLQLTAR
                     LESTLTAETVLKLYEDGVEVESRPVKLAPGTEQVETFTRSPEGGSIHRYRAVLMNTPG
                     DSIPANNAALALVEVRGRLRLLALEGNTAEGAYLEQAMAKEGIEIELRAPGRFPSALD
                     QLAGYDGVILSDVPAHRLGEPLMNALRDYVDKLGGGLLMLGGPSSFGLGGYHQTPVGD
                     LLPVRLKAPDEEEKQSAAIALVLDRSGSMAGEKLEMAKSAAIATAEVLGRNDSLGVFA
                     FDSEAHTVAPMTRLTSTAAVSGQISALAAGGGTNLEPAFQQAREALLRVKARVRHMII
                     LTDGQTTGGGYEALASQCRGEGVTISTVAIGEGSHVALLQAIASSGGGQAYSTLDPAS
                     ITRIFTQDTLMHAGRMLREEPFDARVVERHPMLAGLEKWTAPPLLGHVKTLRKTTAQA
                     PLTTDLGDPLLAHWRYGLGKVTAFTSDAKSRWASLWLARWPDFSRFWSQVLRETARLP
                     QGRQLDLSLQLDGDEARIRADRLLDAGTRANDARVEAEVFFADAEALGSPLQSRQRLS
                     LEQSGPGLYEGAFRPDRPGVYLVRARSGADMVSAGLVHNPGSESALGTVNGELLAEAA
                     RLT"
BASE COUNT         2105 a         4364 c         3926 g         2037 t
ORIGIN      
        1 gtcagggaat gtttctcccc tttccctgac ggcgggcttg ccgctttccc gtttgcgccg
       61 gagggcgcag cgggaagaat ggcgggttca tgagccttca tcccacacgc cgccgtttcc
      121 tgctcgccct gaccgcgtgc ggagttaccg acagcgtcaa gggggtggag catctgccgc
      181 aaagcgtgac attcaagggc agggagaaat ttgaccggat cgtggcgcgc ggcatcgcgg
      241 aacagtggag ccggctgccg atgggcgcgc gcgtgatgag cgccgcgctt gccatggaag
      301 gcactcctta tgccggattc accctggaga tagacgacca tgtagagtcg ccctcggtaa
      361 atttcgacgg gctggactgc tggaccttct tcgagaccgc cctcggcctc gcgcgcatgt
      421 tcggcagatc gcatggctcc cgcactcccg ccggtctcct gcgcgaaatc gagacgactc
      481 gctaccgtgg tggtctctgc cgtggaggtt atctggagcg catccattac ctggaggaat
      541 ggtttcgcga caacgaccgg cgcggccatg tgcgcgacat caccgccacc ctcggccccc
      601 tcaccgagat caaaggccgg cgcattgacg agatgaccgt gctttggaaa agctaccgct
      661 acctcgccaa taacccggac ctgcgcgcgg gcatggcgcg catcgaggcc gaactccaga
      721 gccatccttt ccactatctg ccaaaggccg ccgtgcaagg catcgagcac aagctcgaaa
      781 gcggtgacat cgtcggcatt gtcacgcaca agccgcatgt ttattgcagc cacgccggcc
      841 tcatcctgcg ggatgccgcc ggcgcgcgcc gcttcatgca cgcctccacc acgcgcaagc
      901 gcgtgctggt ggacaagccc cttcacgcct acctcgccga tttcaaaagc cacgccggca
      961 tcatcgcatg ccgcccactc tgactccgac accgccatgc acgtcccaag cctcatcgaa
     1021 aaaaaacgcg acggccaagc cctcgccgaa gaggaaatac acgccctgat ccgcgccttc
     1081 accctgggcg aactgcccga ctatcaaatg agcgcgctcg ccatggccgt atatttccag
     1141 ggcatgaccg cgagcgagac cgccgccctc acccatgcca tgctccacag cggcatcgtg
     1201 ctgagctggc ccgccggatc accgccgcgc gtggacaagc actccaccgg cggcattggc
     1261 gacaagacat ccctcgttct cgcgccgctg ctggcatgcg acggtctctg ggtgccgatg
     1321 atttccgggc gcggtcttgg catcaccggc ggcaccctgg acaagctgga gtccatcccg
     1381 ggcttccgca cccggctgga ggagaaggaa atccaccgcg tcgtgagcga tgtgggctgc
     1441 gtcatggtcg gccagacgga gggcctgtgt ccggcggaca aaaaacttta cgccctgcgc
     1501 gatgtcactg ccaccgtgcc gagcatcccg ctcatcacgg ccagcatcat gggcaagaag
     1561 ctggccgagg gactcgaccg cctcgtactc gatgtgaagc acggcaccgg cgccttcatg
     1621 cgaaccgagg agcaggcgcg cgcgctggcg gattccatgg tggctgtggg aaccgcgctc
     1681 ggtgtgcgct gcgcggtgcg catcaacgcc atgagcgagc ccaccggccg cgccgctgga
     1741 aacgcgctcg aggtggccga gtgtgtcgag tgccttcaag ggcgcggtcc gggggacctg
     1801 gaggacatcg tgcttgaact cgccggcgcc gtctccgaca caccccgcgc cacgcacgag
     1861 caacgcctgc gcgatggcag cgcgtggcgc aagttccagc aaatgaccgc cgcccagggc
     1921 ggggacattt ccgtcctgga aaaaatcacc gaagtccacg ccgcccccgt cattatcgag
     1981 gtgcccgcgc ccgcaagtgg cattgtgcgg cgcatggacg ctggaaccat tggccgcgcc
     2041 gtgctcgctc tcggcgctgg ccgttcgcgc gcgagtgacc cggttgactt cgccgtcggc
     2101 gtggaccgca tcgttaaaac gggggaatcc gtgacggcag gcgcgccttt gctgcgcgtc
     2161 catgcccgca caccggccgc ggcggaagcg gccatgcgta cgattttgga aggcgtggaa
     2221 atccgatgag aggaagaaag gcccgggacg gccctcgtgc gccatcccgc ttgttcaaag
     2281 agcgtgtttg acaatcgccg ccactggcgg cttgaatccc gcgccgcgca tcgtttttgg
     2341 cggcaccggt ctcgaatgca aaagctggaa tccatccccc aactcggctg cctgcgcggt
     2401 ccgttggcgc ttgccatcgg agtcttcgac gggctgcacc tgggccatca ggaggtgata
     2461 ctggcggcgc gcgagcacgc ggcgcggcat gaggggaggg cggtggtgct gaccttcgac
     2521 ccccacccgt tgcgcgtcct gcgccccgag tccgcgccgc tgcgcctgtg cggctcgcgc
     2581 tatcgcgccg tccttctcga acgcatgggg ctggatgcca tgctggtgtg ccccttcacc
     2641 ccggagcttg cgtccaccga ggcggaggat ttcgtcgcgg agttggtggg ggtctgccgt
     2701 ccgctgggct gcgtctcggt gggttacgcg tggacctttg gcaaaaaccg cggcggcacc
     2761 atccactcgc tcatggacct cggacagagg cacggcttcg cagtctatgg tgttccgccg
     2821 gtgcgggtgg atggcgaagt ggtcagcagc acgctgatcc gccaagccgt ggctgcggga
     2881 gatctcgcgc gggcggcggc tttgtcgggc cggccctacg cattgttcgg caaagtggtg
     2941 gaaggccgga aactggggcg cggactcggc ttcccgaccg ccaatgtgcg cgtggaggcg
     3001 gagatgctgc cgccctacgg cgtgtatgcg gcgagcgccg acatcggggg caggacactg
     3061 gccggagtgg cgaacctcgg ggtgcgcccg acggtggaaa agaccggctc agtccccatg
     3121 ctggaggttc atttcttcga ctggcagggc gatctctacg cgcaggatct cgaagtgcgg
     3181 ctgggagcgt tcatgaggcc ggagagaaaa tttgccggca tggcggaact gcgcgcgcag
     3241 atcgcctcgg atgtgctgct ggcgcgctca gcgcccctcc ccggctgaag cctggggcgc
     3301 ctcgaagttc agcaccacgg gacggtggtc gctggccttg tcaaaatccg cgcttgtgtg
     3361 aatgcggctt tttttcgtgt ccaccagggg cctcagattc cggctgacaa agaggtagtc
     3421 gaggcggctg taaacatccg ccacatccca aaagtgggtc caaagaaggc cgtgcgagtc
     3481 tttgacgggt atctccagca tgtagctctc ctgcgccctg ctgccgatga tctcatccat
     3541 cgaagactcg ttgcggtatt cattgaagtc gccgtagagc accaggtttg gccgcgattg
     3601 cagggagaag attttgtcga ggtgcgcgcg agtcagatgc gcctcgttcc gcctcatgag
     3661 cgcctggtcg ccctcggcca cctcgcgctt ggacttcaga tgcacgccca gaaatcgaac
     3721 ctggaaatcc ggacggatgt ccacagtggc atccaggatg ccgcgctgga aaaaaaaagt
     3781 ctcgccgcca atctggtagg gcagagcgcc ctggtgatgg cgcgccgaga tggggaaacg
     3841 cgagagcagg gccagactgc gcgtcacatc cgcgccctgg ttgaactcgc tgtgcggcag
     3901 gtccatgccc gcgtctttga gccgcgactg cagttcctgc aaatcctcgc gcgtgccgat
     3961 ctcgcagaca ccgagaatgt cgggctgtat ttcggaaagg aattcaatga ccttctgctt
     4021 ttccgcatca ggcttcgact ggggaggccg tttgctcccg ccaggagact ccatcaccag
     4081 ccagttcttc aaattgtaag cgcaaaaagt cactggctca cccgcctgtg aagcaaccac
     4141 gcccagcacc aaaaaaaggg cggacccgag ggcccgccat gtgattcccg tcgaactcat
     4201 ccaggaaaag gcgtgatgat caaacgtcct gggaggagcc gacgggctgg cgcttttcag
     4261 ccgtcgcggg aatcgtcggt ttttcgactt cctcctgggc gcgggtcaac tcgtgctcga
     4321 actcgtcacg cgcttttttg aactcaccca tgctcttgcc gaggctgcgg gcgaaggtgg
     4381 gaagcttttt ggcaccgaaa agaaccagca caaggatggc gatgatgatc aactcgggtg
     4441 tgccgagagg accgaaagcg gcaaggatgg aaggggtgtt catgatgggg gatggttatg
     4501 attgaaaacg tccccgcgca agcccgcctt tcggcagatt gccagtgaac aaatgaagca
     4561 tccatgccag actgcttgcc gtaaacagcc cgaatcatgg cgaagccccc cgacgccgca
     4621 tccccaaact cccccgactc ctccgccctg cacgagcatg atgtgcagac tctcgcgcat
     4681 ttgtgcgggt tgaccggcac tccctttgac cggatgcgcg ccagccaggg tctgcacaat
     4741 gccgcgcgct cttgcaagga gcctctggaa cgcctcgcgg cagccgccag tgaatgcggg
     4801 atcagcatca ccccggcgcg tctcacactg gcggatgcca tctggcgcgc ggcggactcg
     4861 gtgccttttg cggcctggtc agccaagagc caaagctggc tggtgataca caagcacggc
     4921 ttttttaaag cgcgtgtctc cgaccctgaa aacccatcgg aaacagaaaa cctcacacgc
     4981 ggcgagctgg cctcgcgcct tggactcgcc agcgcggagg cgccggctga tttcggcatc
     5041 gtgagcgcgg aactgccgat gcgcgcgctc ggacaagatg ctcatcacgg gcacgccgcc
     5101 gtctctcccg tccgccgctt catcgcgctc atgcggcctg agagaaagga catcggctcg
     5161 gtcatcattt tcagccttgt caccggcgtg ctttatctgg cgctgccgtt ggcggtgaac
     5221 gcgctggtca gcaacctcgc tttcggcggc cagtccggcc cgctgtggac ggcgctgctc
     5281 atcatggcct tcgccctcac gctggcgctg ctgctctccg ccttcatccg cgccatgcag
     5341 tactacatcg tggagatcat tcagcggcgg ctctttgtgc gcctggccgc cgatctctcc
     5401 caccgcctgc cgcgcgtgtg cgcgtcctct ctggacggcg ttcacgcgcc cgagctggtg
     5461 aaccgtttcc ttgatgtcgt caccgtgcag aaaagcaccg ccctcatgct gctcgacggc
     5521 atcaacatcg tgctcggcat ggtcgtgggc atgaccgtgc tcggccttta ccacccctac
     5581 ctgctcggct ttgtcatcct gctgctggcg ctgctgctgg gcaccatgct cgtgctcggc
     5641 cgcggcgcgg tgcgcacaag cgtgggcgag tctcttgcca aatacgaggt cgtcggctgg
     5701 ctagaggagc tggcgcgcta cccgcggctt ttcaaaggcc ccggcggcta ccgcatcgcc
     5761 gccgagcgcg ccgacgacct cacacgccgc tacctcgaca accgccgcgc gcatttccgc
     5821 gtgctctacc gccagatcgc cgggctgctc gtcatggaag tcgtcgccag cgccgccctg
     5881 ctcatcgtcg gcggctggct cgtcatcagc caggagctca ctctcggaca gctcgtcgcc
     5941 agcgagctca tcgtcggcac catcgtggcc tccctttcga agctggggaa aaaattcgag
     6001 gcctggtatg acgccatggc cgccatggac aagctcggcc acctcgtgga cctgcccgtc
     6061 gagcgcgagg acggcgagtt ccccccgcgc aagccggacg gcgcgcgcct cgaagtcggc
     6121 accctcggct acacctaccc gtatggcgtg ccggtcttca ccggcctttc cttcgaggtg
     6181 gcacccggcg agcgcgtggc cctcaccggc acccagggca gcggctccag caccctgctc
     6241 gacatcctcc acggcgggcg cgagccttcg gaggggtaca tccgcctcga cggcctcgac
     6301 ctgcgcagtt ggtatctcga agccctgcgc gagcaggtgg cgctggtccg tccggacgac
     6361 atcttccagg gcagcgtggt ggaaaacaca cgcctcggca acatgagcat caccatggac
     6421 gaggtcaccg ccgcgctcga ccgcgtgggc ctgctgcgcg agctgctgga gatgcccgac
     6481 ggcgtgaaca cgcagctcat caccggcggc ctgccccttt ccagccgcca gcgcatccgc
     6541 cttgtgctgg cgcgcgccct cgtcacacgg cctcgcctgc tgctgctgga tgaaattttc
     6601 gacggtctcg acgacgccag catggacgag ctttgcggca tcatccttga tgaaaaaaat
     6661 ccatggaccg tcctcgtcgc cacgcgcgac gccgccgtgc tccgccgttg ccagcgcgtg
     6721 ctggacctca gccgctttgc cgaagccgcg catccaccca cccaagcccg atgaaagcgc
     6781 acgccatacc cactctggag cttcgcggca ggctgccctg catggcgctt gccggcagct
     6841 cccgcttcag ccgcatcctc gcacgtgtgc tggccatcct gttcgttctt tccctcatcg
     6901 gcgtcttcgt gctgccctgg cagcaaaacg tgcccggcgc cgggcgcgtc atcgcctttg
     6961 atcccctgga gcgccgaatc aatgtcgagg cgcctgtctc cggccgtgtg aaaaaactcc
     7021 acgtcgtcga aggccagcgc gtgaagaagg gcgacatcct cgtcgagatc caggacaacg
     7081 accccaacct gctcgccaac ctccgtcttc agcgcgatgc cgccatcgcc cgccgcgccg
     7141 ccgccaagca gcgtgtcgaa gacctcaccc agcaggccca ggaaatggaa ctggcaaaaa
     7201 gccaggccat tgatgccgcg cgccagcgca tcgccgctga aaaaatcacc gccgaaaccg
     7261 ccgtgctgaa ccaccagcgc accgtcaccc tggctgaaaa aggcctcgtc tccacgcgcg
     7321 acctcgagct ggccaccctc gcgcgcgaca gctccgccgc caacctcgcc gccgccgagg
     7381 ccaccctcac ccgcaccgag cgcgagatgg aggcatccat ctccaacatc cgagccaacc
     7441 gcggcaccgc ccagggcgat gtggccgccg ccgagcgcga gatggcctcc attgactctt
     7501 tgatcagcca aaacctccag caagtcatcg aatccccgcg cgacggcatc gtgctcagcg
     7561 tcacagccac cgacggcacc ttcctccgac ccggctcccc catctgcgtc gtcatccccg
     7621 agaccgagag ccgctttgtc gaactcatgc tcgacggcaa tgacatcccc ctcgtcaccg
     7681 cgcgccaggt tcacgaggac ggcaccatca ccgaaggcag ccccgtgcgc ctgcaattcg
     7741 aaggctggcc cgccgtccag ttcatcggct ggcccagcgt cgccgtcggc accttcggcg
     7801 gcgaggtcgt ctttgtggac gccaccgacg acggcatggg ccagttccgc gtcgtcgtcg
     7861 cccccaagcc ggatgtcatc gtgcggcgcg gtgttgagcg cgtcgagccg tggccgggca
     7921 accgctggct gcgccagggc gtgcgcgcca aaggctgggt gctgctccag caggtgcctc
     7981 tctggaagga aatctggcgc cagctcaacg gcttcccccc ggtcatctcc aacaccgaac
     8041 ccgtggagaa aaaaacgtga accacacccc cagtccccgc attctggcga atgccccgcc
     8101 ccgggtaggg cgggctgtcc ccagcccgcc gcgtgcgggg acgcagcgta cgaatgacgt
     8161 tgaaccaccc cgcgtacccg ccacctgcgc tcgcggcgcg ttgaggacaa cgcgccctac
     8221 cctgcgccgc attctggtga atgccccgca ccaccccgca ggcgcgcttg ccaaaagcgc
     8281 ggagaacgct cacgcaggca cgcgcagttt ccgcgcctcg gcaaaggact tcacagattc
     8341 gtgtctattc gtgtccattc gtggttcctc cctctcccgc attctggcga atgcgcctac
     8401 cgccccgcgt aggcgcgctt gccaaagcgc agagtttgct cccacccgca gcccgcgcac
     8461 ttctccggtt gccgcgccca atccatgaac cgttaccgcg ccctccgatc cagcctcctg
     8521 gcgcttctgc tcgccgccac cacacccgcc gcggcggaaa agcccctcac gctcgacgag
     8581 gtgctcgcct ccgtgaagag tacctacccg ccctatctcg ccgccttgat cgagatggac
     8641 atcgccaacg gccgcgtgcg ccaggctgct ggcgcttttg acctcaacct cagcgccggc
     8701 ggcagcttca ctccctccgg ttactacgac gggcgcagcg gcgccgcgct cctggagcag
     8761 cccctgccct tctggggcgg cagcctcttc ggcggctacc agctcagcag cggcttcctg
     8821 ccgaactaca acaaagaccg cacccagcgc gacggcgagg ccatcctcgg cttcaagctc
     8881 aacctcctgc gcgacggcac gctcgaccgc cgccgcgcgc agctctacca ggcccgcgtg
     8941 gaccaggaac tggccgatcc actcatcctc cgccaataca ttgacttcat ccgcgccgcc
     9001 tccatcgcct attacaactg gctcgccgcc ggccaccgct ggcagctcgc cgaggagctg
     9061 ctgcgtgtcg caaaagaccg ggatgaagcc atcgccgagc aggccgggcg cggcgccgtc
     9121 gcccccatca tctgtgtgga caacgagcgc ctcgtcgtca gccgccagat cagcgtcgtc
     9181 caggcccgcc gccgcttcga ggccgccgcc atcgagcttt ccctcttcca ccgcggcgag
     9241 gatgacgagc cagtcatctc cccccgctcc cggcttttgt ccgccttccc cgagccgccc
     9301 gtgcccgacg ccacccgtct ttccagcgac atcgccaaag ccctcatctt ccgccccgag
     9361 atccgccgcc tcgacctcac cctcgaaaaa gccgacatcg agcgccgcct cgccaaaaac
     9421 aacctcctcc cgaacctcga cctcaccctc caggccaacc aagccgtcag cgagcgcacg
     9481 caaaaggaca tggagcgcct cgaaatggag gccaaaatcc agttcagcct cccactccag
     9541 cgcaacgaag ccaaaggccg cctcgaaacc atcgaagccc agatgagccg cctccagacc
     9601 gagcgccagt tcgcccgcga ccgcatcagc gccgacgtgc gcgacgccat gtccgccgtg
     9661 caggccgcgc gccagcagat cacccagacg aggcgcaatg tcgaactcgc cacccagatc
     9721 gagcaagccg aggccctcaa attccagcag ggtgccgccg acctcctggc cctgcaaatc
     9781 cgcgagcaag ccaccttcga cgcgcgccag ctcgatgtcg aagcccacgc cgaatacctc
     9841 cgcgccctcg ccaaccaccg cgccgccatc gcgaccgacg ctcccgcttc actggcgcgc
     9901 tgagccgccg cacggaaaat tccaaaatga atggcgtaaa ctggcgaagg gcgcatgact
     9961 caagttcgcg cgtgccggga caattcgcgc tatgaagacg gcgcttcctc cgtagcatcc
    10021 cgccgccctg atggactggg aatccccaaa actgctcctg ctcatcctgc ccgccctgct
    10081 cctgctgctg tggatcgagg gcgcgtccgc gcatcccatg actgggctgc gcaagcggct
    10141 gctgctggtg gcgcgcgctc taggcgtgat gctggcactg gcggcgctgg cggggccggc
    10201 gcgtgtgagc gagtcggggc ggaagtccct gggcatcgtg gtggatgcca gccaaagcct
    10261 tggggcggag ggttatgcgc gggcgctggc agaggcggag cgggtgcgcg cgggtgcggg
    10321 tgtggagact ttcgtggtgc gcttcggctc caggccggag ctgctgccgg ctgaggcggc
    10381 ggagacgccg gagcaggcgg cggcctggcg ggagaaaaac ggcggggaca gccattacgc
    10441 cgccgccttg gagctggccc gggcgctttt cccgccgggg gccagccgca cgctcctgct
    10501 cataggggac gggcacgaga cgcgcggcag cctgctggac actgcgaggg atgcggcgct
    10561 ggcgggagtg cgcctgcaca cgctgccggt ggcagggacg ctgaggccgg acgcgcgcgt
    10621 gctctccctg acaccctccc gcccgcgcct caacgagggg gcgaccctgc aactcacggc
    10681 gcggctggag agcacgctga cagcggagac tgtgctgaag ctctacgagg acggcgtgga
    10741 ggtggagagc cgcccggtca aactggcacc ggggacggaa caggtggaga ccttcacccg
    10801 ctcgccggag ggcggcagca tccaccgtta ccgggccgtt ttgatgaaca cgccggggga
    10861 ctccatcccg gcgaacaatg cggcgctggc gctggtggag gtgcgcggac gcctgcgcct
    10921 gctggcgctg gagggcaaca cggcggaggg cgcgtatctg gagcaggcga tggcgaagga
    10981 ggggatcgag atcgagctgc gcgcgccggg acgcttcccg tcggcgctgg accagctcgc
    11041 gggctacgac ggggtcatcc tctccgacgt gcccgcgcac cggctgggcg agccgctgat
    11101 gaacgcgctg cgggactatg tggacaagct gggcggcggg ctgctgatgc tgggcggccc
    11161 gtcgagcttc ggcctgggcg gctaccatca gacgcctgtg ggtgatctgc tgccggtgcg
    11221 cctgaaggcg ccggacgagg aggagaagca gagcgccgcc atcgcgctcg tgctggaccg
    11281 ctccggctcg atggccgggg agaaactgga gatggcaaag agcgccgcca tcgctacggc
    11341 cgaagtcctg ggccgaaacg actcgctggg cgtgttcgcc tttgacagcg aggcgcacac
    11401 ggtggcgccg atgacgcgcc tgacctccac cgccgctgtg tccgggcaga tctcggcgct
    11461 ggccgcgggc ggcggcacga atctggagcc ggcttttcaa caggcgcgcg aggcgctgct
    11521 gcgtgtgaag gccagggtgc gccacatgat catcctgacg gacggccaga ccaccggcgg
    11581 cggttacgag gcgctggcct cccagtgccg gggggagggc gtcaccatct ccactgtcgc
    11641 catcggcgag ggctcgcatg tggcgctgct ccaggcgatc gcctcctcag gcggcgggca
    11701 ggcttattcg acgctggatc ccgcctcgat cacccgcatc ttcacgcagg acacgctgat
    11761 gcacgcgggg cgcatgctga gggaggagcc tttcgacgcg cgggtggtgg agcgccatcc
    11821 gatgctcgcg ggcctggaga aatggaccgc gccgccgctg ctcggccatg tgaagacgct
    11881 gcgcaaaacc accgcccagg cgccgctgac caccgatctg ggggacccgc tgctggcgca
    11941 ctggcgctac ggcctgggca aggtcaccgc cttcaccagc gatgcgaaga gccggtgggc
    12001 ctctctctgg ctggcgcgct ggcctgactt cagccggttc tggtcgcaag tcctgcgcga
    12061 gacggcgcgc ctgccgcaag ggcgccagct cgacctctcg cttcagctcg acggggacga
    12121 ggcgcgcatc cgcgcagacc ggctgctgga cgcgggcacg cgcgccaatg acgcgcgcgt
    12181 cgaggcggag gtgtttttcg cggacgccga ggcgctcggc tcacccttgc aaagccggca
    12241 gcgcctttcc ctggagcaaa gcggccccgg cctctacgag ggcgccttcc gcccggaccg
    12301 gcccggtgtc tatctggtgc gcgcgcgcag cggcgcggac atggtctccg ccggcctcgt
    12361 ccacaatccg ggctcagagt ccgctctcgg cacggtgaac ggggagctgc tggccgaggc
    12421 cgcgcgcctc ac
//
LOCUS       DAOMYJ010000157        12391 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Prosthecobacter sp. isolate AMR_MDS_5629
            NODE_1706_length_12391_cov_23.825065, whole genome shotgun
            sequence.
ACCESSION   DAOMYJ010000157 DAOMYJ010000000
VERSION     DAOMYJ010000157.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867850
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prosthecobacter sp. (wastewater metagenome)
  ORGANISM  Prosthecobacter sp.
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Verrucomicrobiaceae; Prosthecobacter.
REFERENCE   1  (bases 1 to 12391)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12391)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.30
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:34:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,742
            CDSs (total)                      :: 4,684
            Genes (coding)                    :: 4,676
            CDSs (with protein)               :: 4,676
            Genes (RNA)                       :: 58
            tRNAs                             :: 55
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12391
                     /organism="Prosthecobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1706_length_12391_cov_23.825065"
                     /isolate="AMR_MDS_5629"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1965333"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            44..1489
                     /locus_tag="PLP58_15215"
     CDS             44..1489
                     /locus_tag="PLP58_15215"
                     /inference="COORDINATES: protein motif:HMM:NF018178.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1080 domain-containing protein"
                     /protein_id="HRJ09926.1"
                     /translation="MPLHPALLSFLAAGTALAAEPGFDPIFDGKTLDGWKHGGNWAVV
                     NGEIACLGKGGSLVHERASVPDDFELRFDWKVSKGCNSGVYYRPGQYEYQLLDNANSP
                     YGENPRQAAASLFFGMAPAKDAVKPHGEWNEGRIVCKGTVIQHWLNGEKVIDFDYSDP
                     RWHNEVEVLRIRGGDLAARGGNISLQYHGADVWFKNLRWRAIPADEELVCENLAPMPI
                     PDTALRKEQDRVNGMLEKAGKGGWTHEELRRFKVDEAHQGVAVDGAHFYAITNTAIGK
                     YRKDTGGRIGGWQAEKGGRFKHLNAGVVLDGKLWCAHSNYPELPMRSSVEIWDAATLE
                     HLQSVDLTSAGGSLTWVDRRDGWWYACFAQYARTGDPARTRVVRFDLNWKPAGELSFP
                     PEMVAKFGKNSSSGGSFGPDRHLYITGHDAQELYVLSQPVTGDAWTWRGVIPITAHGQ
                     AFAWDRAKPGLLYSIDRGTKEVIVSRVRPLD"
     gene            complement(1517..2284)
                     /locus_tag="PLP58_15220"
     CDS             complement(1517..2284)
                     /locus_tag="PLP58_15220"
                     /inference="COORDINATES: protein motif:HMM:NF012546.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HRJ09927.1"
                     /translation="MKKFLALFLTLATSALAQEALPKKPEIFEVEGHKAVLYAAPKPA
                     EGKPWVWYAPTLNGGVSLAGRKMYFEAFMKAGISLAGFDLGEVRGSPASSAKFTHFYD
                     AMVKRGFSAKPILLGQSRGGMMTLAWAFRNPDKVKAWAGIYPVCNLTSWPLKNSKAQT
                     LADFALPEAEIIARLKEFNPIDNLAGLAANKVPMFAVHGDSDVVVPYDDNTRLLKERY
                     EAAGGSISVKIIPGEGHKATPSFFECQELVAFVLGAR"
     gene            complement(2288..3589)
                     /locus_tag="PLP58_15225"
     CDS             complement(2288..3589)
                     /locus_tag="PLP58_15225"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="sialidase family protein"
                     /protein_id="HRJ09928.1"
                     /translation="MKSTILALLIAGTPLFAQIRPLAQEHVTIWESPDPARIYGYTPG
                     ICRLPSGRLVVTHEISSASKPVPPEGKAFHESRILTSDDGGKTWTHRANFDLSFARPF
                     VAGKSLYILGRDKQVAVIRSDDEGVTWSERSFLNDTGIWHQSACNVHFAKGNVYLVME
                     KHAPKRGIQGWQVADLAPVLMRAKADSDLTRRENWTFSSELVFEDIPEARLPNAFGLP
                     FHPMDRKKVTFLVPPKGRGTAPLGWLETNVVQFTDPTHLWHDPQGKTFHLWMRANTGM
                     TNYAAMAQVRENDDGSMTTSLVKSPAGTPMLFVPCPGGQMRFHVLWDDQTKLFWLLSS
                     QTTDSMIRPDAMPKDRWGTPDNERHRLVLHFSKNMIDWCFAGLVTKGETPKESRHYAS
                     MCIDGDDLCIVSRSGNAKAHSAHNGNLITFHRVHRFRALIY"
     gene            complement(3586..4974)
                     /locus_tag="PLP58_15230"
     CDS             complement(3586..4974)
                     /locus_tag="PLP58_15230"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase"
                     /protein_id="HRJ09929.1"
                     /translation="MCSSALLGAAPPNIVFILSDDLAWSDLACYGHPYHHTPNLDRLA
                     SQGMRFTQAYAPAPICSASRAAILTGKTPARLNFEFVTKDKPGGQNLKQPLESPPYTL
                     NLPLKETTLAEVLAPAGYATGFYGKWHVSQHHNGYLGWSPTHGPLQQGFAEGTGEFGS
                     HPYGYKAQPELKNTPAADGTFPEDALTDAALDYLKKHRDERFFLYLSQYFVHDPVHSR
                     CQWLIEKYRAKLPPGSLDIRASYAAMVETLDRLVGRVLDELDALKLADNTLVVFMSDN
                     GGHPNYTANTPLRGSKWNLYEGGIRVPFIVRWPGHTPAGTVCNEPVHGCDLLPTFAEL
                     ASAQTGAVDGVSIASLLRDPASKLPERPLLWHFPYYHPEKGFAKAPSHIGTSDFVTSQ
                     TRPHSALRLGRHKLLHFDEDDRDELYDLEADPGEAANLSTSRPELTAQLRERLFSMLK
                     ACGARFATPSKP"
     gene            complement(4998..6353)
                     /locus_tag="PLP58_15235"
     CDS             complement(4998..6353)
                     /locus_tag="PLP58_15235"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arylsulfatase"
                     /protein_id="HRJ09930.1"
                     /translation="MIPRLLVIFLSVVVSAHAAPPNIIFILADDLGYGELGCYGQKLI
                     QTPNLDRLAAGGTRFTQVYAGSCVCAPSRCCLMTGMHNGHARIRDNVPHGTFLKNADI
                     TMAEVLHEAGYRTGAVGKWSLGIHGSEGKPNDQGFDDFYGHVDQDQAHFYYPDHLWDN
                     DRVVLLPGNRGEKKQQYTADLFAERALKFIRDSAEQPFFLFYASTLPHWSDYPLKSDE
                     SQIVPSDAPYTDRDWPQVEKNYAAMVTMLDRDVGRLMDLLDESGLANDTLIFFSSDNG
                     PSAEAKHKPAFFGSAGALRGVKRDVYEGGIRVPMLVRWPGHVPAGKVSDEVWTQWDVM
                     PTLAEIVGAKITHPIDGHSILTALKGGKAPPHDYLYWDYGHSRGQYKQAARKGDWKAV
                     RNGTDQPIELYDIRYDQTEIQDVAAKHPDVVAQMEEILATASTPDPAYPIAPPKAVRK
                     K"
     gene            complement(6516..8744)
                     /gene="recQ"
                     /locus_tag="PLP58_15240"
     CDS             complement(6516..8744)
                     /gene="recQ"
                     /locus_tag="PLP58_15240"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007418722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase RecQ"
                     /protein_id="HRJ09931.1"
                     /translation="MPMTSDLATLLKEHFGYDSFRPLQKEIMDASLANRDVVAILPTG
                     AGKSLCFQLPALAREGLTLVISPLIALMKDQVDALTASGVAATFLNSSIHGSEAHRRR
                     SGLENGHYKLLYAAPERVMMDGFISDLQRWNVTAIAVDEAHCISEWGHDFRPEYRQLA
                     TLRDRLPGVPFLALTATATEQVRGDIIRQLHLHEPEVFLASFNRPNLSYSILPKAKST
                     RQVYEFVRQRPEEAGIVYVQSRKSAESLAAALSAEGVKAVAYHAGLQPEERAANQEAF
                     IRDEARVVCATIAFGMGINKPDVRYVIHADLPKNIEGYYQETGRAGRDGLPSECVLLY
                     SRGDLVRNLKFLDEMTDAKAADIAARQMRMMADFAEGAECRRVALLDYFGEQWPGDNC
                     GGCDICLQPRETWDATTHAQKLLSCIFRIKQKSGFSTGLNHVVEVLVGANTEKIRKWY
                     HDQLSTYGIGKGTPREEWAALGRQLMRLGYIDASPDTFQTLTLSKQGLATLMNRTPVM
                     LTRVPVVVKSDTSTGKVAKAGSIACDEALFAELRALRKKLADERGVPPYVVFGDTTLR
                     HMCRRYPRTERDFLALPGVGSQKLADYGAVFMGAISEWLETHEAMRFADETPPPPPPK
                     MKSEGGLTGTALETLRLHRQGHNVEKIASLRGFVASTIHNHLSQAIQHGELKAEPRDY
                     FTAEEEAEIRDAAAEHGLESLGKLKEALGNRFDYPVLNYFRAFEQREKPQRADESFPD
                     GS"
     gene            complement(9021..9452)
                     /locus_tag="PLP58_15245"
     CDS             complement(9021..9452)
                     /locus_tag="PLP58_15245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009962484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HRJ09932.1"
                     /translation="MKHIVTLLCILGFSLHAADVAAILAADDARIAAMKAPDMGKLSA
                     IFSDDLHYAHSNGVVDTKSSFVDILVSGRTRYLEYDHVERKTSFPTPGVALVSGRARI
                     QAASEKGVMDSVLSYLAVWRLEDGKWRFLAWQSCKLPPANP"
     gene            complement(9477..10568)
                     /locus_tag="PLP58_15250"
     CDS             complement(9477..10568)
                     /locus_tag="PLP58_15250"
                     /inference="COORDINATES: protein motif:HMM:TIGR00367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0098662 - inorganic cation transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="calcium/sodium antiporter"
                     /protein_id="HRJ09933.1"
                     /translation="MDLITLVLFVIGLALLIFGADLLVRGAARLAAAFGIPPLVIGLT
                     VVAFGTSSPELAVSVQSAWAGQPDLAVGNVLGSNVFNVLLILGLSAVITPLAVSRQLV
                     RLDVPIMIACSALLLLLARDRLISRLDGVIFTSGLIVYVVFLFLQAGKEKNDKEGDFE
                     EEFGQADSGAKAVMTNLFFVAAGLGMLVLGSHWLVEGAVMLARAMGVNELVIGLTIVA
                     AGTSLPEVATSVIAALKGERDIAVGNVVGSNIFNILCVLGLSSIAAPDGLPVSEAALN
                     FDIWVVLAVAVACLPIFFTFGCTIRRWEGLLFLGYYIAYVVYLFFDATKHESLPGYRM
                     MMMVFVVPLTVLTLAVTVIHEWRARASRK"
     gene            complement(10665..10925)
                     /locus_tag="PLP58_15255"
     CDS             complement(10665..10925)
                     /locus_tag="PLP58_15255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ09934.1"
                     /translation="MITSLLDNCIMLWGSGLEDGNKHTRENLPFIIAGKGGGSINTGR
                     FINTVKGNQGDLLTTLLACSGVPLDRPIGIATRQIPGMMKAG"
     gene            complement(11105..>12391)
                     /locus_tag="PLP58_15260"
     CDS             complement(11105..>12391)
                     /locus_tag="PLP58_15260"
                     /inference="COORDINATES: protein motif:HMM:NF012200.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choice-of-anchor D domain-containing protein"
                     /protein_id="HRJ09935.1"
                     /translation="EYGTTTSYGTSTAAQNPGAGLNPIAVNQAVTGLLDGTLYHYRIV
                     AQNAGGTSYGMDMTFTTLIDPEIVVEEPVGLDLVDDTASVGYGDVLLDSNAVKTFTVR
                     NTGTTPLRNLAITKDGTNAADFTVSAISTPVSAGGSTTFTVTFAPGALGARAAAIHIA
                     SNDVDENPFDIALTGTGVTPEIGLEQPAGTNLVDGSASIGYGAVAIGSSNAKTFTIKN
                     TGTAPLAGIAVTVDGTHSGNYVVSSSGMSTTVAIGGSTTFTVTFTPTGTISSTRTAAL
                     HVASSDLNENPFDIALTGDAYSTTADADGDGLNDWAEHNYTALGFNWQVSQPALVTNL
                     FSNLGKAETNANAAGYFSTTQVQALHVGAPLLQRHPATGVFTLTMGVEKSATLAPASF
                     TLFPMSASQTLINGEGRLEFQFTVPDNAAFFRLESN"
BASE COUNT         2303 a         3819 c         3923 g         2346 t
ORIGIN      
        1 ctttgcctga aaggagccgc cctgccgcgc cgctcaaaca tggatgcccc tccaccccgc
       61 actgctctcc ttcctggccg ccggaaccgc cctggccgcc gaacccggct ttgatcccat
      121 cttcgacggc aaaacgctgg acggctggaa gcacggcggc aactgggcgg tggtgaacgg
      181 ggaaatcgcc tgcctgggca agggcggatc gctggtccat gagagggcat cggtgcccga
      241 cgacttcgag ctgcgctttg actggaaagt ctccaaaggc tgcaacagcg gcgtctatta
      301 ccggcccggc cagtacgagt accaactgct cgacaacgcc aacagcccct atggcgaaaa
      361 tccgcgccag gctgcggcct cgctcttttt cggcatggcg ccggcgaagg acgccgtgaa
      421 accgcatggc gagtggaacg aggggcgcat cgtgtgcaag ggcacggtca tccagcactg
      481 gctcaatggc gaaaaggtca ttgatttcga ctacagcgac ccgcgctggc acaatgaggt
      541 cgaggtgctg cgcattcgcg gcggcgacct cgcggcgcgc ggcggcaaca tctcgctgca
      601 ataccacggc gcggacgtgt ggttcaaaaa cctgcgctgg cgggcgatcc cggcggatga
      661 ggaactggtg tgcgagaacc tcgcgcccat gcccatcccc gacaccgccc tgcgcaagga
      721 gcaggatcgc gtgaacggca tgctggaaaa ggccggaaaa ggcggctgga cgcacgagga
      781 gctgaggcgc ttcaaagtgg acgaggcgca ccagggcgtg gccgtggacg gcgcgcactt
      841 ttacgccatc accaacaccg ccatcggcaa gtaccgcaag gacacgggcg ggcgcatcgg
      901 cggctggcag gctgaaaaag gcggccgctt caagcacctc aacgccggcg tcgtgctcga
      961 cggcaaactc tggtgcgctc actcgaacta tcccgaactc cccatgcgca gctcggtgga
     1021 gatctgggac gccgccactc tggagcacct ccagtccgtg gacctcacca gcgccggcgg
     1081 ctccctcacc tgggtggacc ggcgggacgg ctggtggtat gcctgcttcg cgcagtatgc
     1141 gaggacaggc gacccggcgc ggacgcgcgt ggtgcggttt gacctgaatt ggaagcccgc
     1201 gggcgaactg agcttcccgc ctgaaatggt ggccaagttc ggcaagaaca gcagctccgg
     1261 gggcagcttc ggccccgacc gccatctcta catcaccggc cacgacgcgc aggagcttta
     1321 tgtgctcagc cagcctgtca ccggagatgc ctggacctgg cgcggagtca tccccatcac
     1381 cgcgcacggc caggccttcg cctgggaccg cgccaagccc ggcctgctct actccataga
     1441 ccgcgggacg aaggaggtga tcgtctcccg cgtgcgccca ctggactgag gcagcgcatt
     1501 caacctcccg ccggagctac ctcgccccga gcacgaaagc caccagctcc tggcactcga
     1561 agaatgaggg tgtggctttg tggccttcgc cggggatgat tttcacactg atcgaacctc
     1621 cggcggcctc gtagcgctct ttgagcagcc gcgtgttgtc gtcatagggc acgacgacat
     1681 cgctgtcacc atgcacggcg aacatgggaa ccttgttggc ggcgagaccg gcgaggttgt
     1741 caatcggatt gaactccttc aaccgcgcaa tgatctccgc ctccggcagg gcgaagtcgg
     1801 cgagcgtttg cgccttggag ttcttcagcg gccagctcgt gagattgcac acgggataga
     1861 tgccggccca ggctttgact ttgtccggat tccgaaacgc ccaggccagt gtcatcatgc
     1921 cgccgcgact ttggccgagc aggatgggct ttgcggaaaa gccacgcttc accatggcat
     1981 cgtaaaagtg cgtgaacttc gccgagctgg ctggcgagcc gcgcacctcg ccgaggtcga
     2041 agcccgcgag actgataccc gccttcatga aggcctcgaa atacatcttt cgccctgcga
     2101 gcgaaacgcc gccgttcagc gtcggcgcat accagaccca cggtttaccc tcggcaggct
     2161 tgggcgcggc atagagcacg gctttgtggc cttcgacttc gaagatctcg ggctttttgg
     2221 gcagtgcttc ctgagcgagc gccgaggtgg cgagggtgag aaagagggcg agaaactttt
     2281 tcatggttca gtagatcagg gcgcggaaac gatgaacgcg gtggaaggtg atcaaattgc
     2341 cgttgtgcgc ggaatgggcc ttcgcattgc cgctgcggct cacaatgcat agatcgtcac
     2401 cgtcgatgca catgctggcg tagtggcggc tttccttcgg tgtctcgcct tttgtcacga
     2461 gacctgcgaa gcaccagtcg atcatgtttt ttgagaaatg gagcacgaga cgatgtcgct
     2521 cgttgtccgg cgtgccccag cggtccttcg gcatcgcatc ggggcggatc atggagtcgg
     2581 tcgtttgcga gctgagcagc cagaagagct tcgtttggtc atcccagagc acatgaaagc
     2641 gcatctggcc gcccggacat ggcacgaaga gcatcggcgt gccggcgggt gatttcacga
     2701 gcgaggtggt catgctgcca tcgtcgtttt cacgcacttg cgccatggcg gcgtagttcg
     2761 tcatgccggt gtttgcacgc atccagaggt ggaaggtttt gccctgcgga tcatgccaca
     2821 ggtgcgtggg atcggtgaac tgcaccacat tcgtctccag ccagccgagc ggcgcggtgc
     2881 ctcgtccttt gggcggcacg aggaaggtca ccttcttccg gtccatcgga tgaaacggta
     2941 ggccaaaggc atttggcaga cgcgcttcgg ggatgtcctc aaacacgagc tcgctcgaaa
     3001 aggtccagtt ctcacgccgg gtgagatcgc tgtccgcttt ggcccgcatg agcacgggcg
     3061 cgagatccgc cacctgccag ccctggatgc cgcgcttcgg cgcgtgcttc tccatcacga
     3121 gatacacatt ccctttcgca aagtggacat tgcacgcgct ttgatgccag atgccggtgt
     3181 cgttcaaaaa cgagcgctcg ctccacgtca cgccttcgtc atcggagcgg atcaccgcca
     3241 cctgcttgtc acgaccgagg atatagagcg actttccggc cacgaagggc ctcgcaaagc
     3301 tcagatcaaa gttcgcgcga tgtgtccacg tcttcccgcc atcatcgctg gtgagaatgc
     3361 gtgactcatg aaacgctttt ccttccggcg gcaccggttt gctcgccgag gaaatctcat
     3421 gcgtcaccac cagccgtcct gaaggcagcc ggcagatgcc cggcgtgtaa ccataaatcc
     3481 gcgccgggtc cggtgattcc caaatcgtga catgctcctg cgccagcggg cggatttgag
     3541 cgaacaacgg cgttcccgcg atcaagaggg caaggatggt tgatttcatg gtttcgacgg
     3601 ggtggcgaag cgtgcgccgc aggccttgag cattgagaac aggcgttcgc gcagttgggc
     3661 ggtcaattcg ggccgggaag tggaaaggtt cgctgcttct ccgggatcgg cttcgagatc
     3721 gtaaagctcg tcgcgatcat cttcgtcaaa gtgcaagagc ttgtgacggc cgagcctcag
     3781 cgcggaatgc gggcgtgtct ggctggtgac gaagtcactc gtgccgatgt gcgagggcgc
     3841 tttggcgaag cctttttcag gatggtaata cgggaaatgc cagagcaacg gacgttcggg
     3901 cagcttgctc gccggatcgc gcagcagcga tgcgatgctg acgccatcga cggctccggt
     3961 ttgagcggag gccagctcgg caaacgtcgg cagcagatcg cagccatgca ccggttcgtt
     4021 gcaaaccgtt ccggcgggcg tgtggcccgg ccagcgcacg atgaacggca cgcggatgcc
     4081 gccttcgtag aggttccact tgctgccgcg aagcggggtg ttggcggtgt agttgggatg
     4141 gccgccgttg tcggacatga agacgaccag tgtgttgtcg gcaagcttga gagcgtcgag
     4201 ttcatccagc acgcgaccaa ccagacgatc cagtgtctcg accatggccg cgtaggacgc
     4261 gcgaatgtcc agcgagcccg gcgggagctt ggcgcgatac ttttcgatca gccactggca
     4321 gcggctgtgc acgggatcgt gtacaaagta ctgcgagagg tagaggaaga agcgctcatc
     4381 gcgatgcttc ttcagataat cgagggccgc gtccgtcagc gcatcctcgg ggaacgtgcc
     4441 atcggccgcc ggcgtgttct tgagttccgg ctgtgccttg tagccatacg gatgcgaacc
     4501 aaactcgccc gtgccttcgg cgaaaccctg ctgcagcggc ccatgggtgg gcgaccagcc
     4561 gagatagccg ttgtgatgct ggctgacgtg ccatttgcca tagaaccccg tcgcgtagcc
     4621 tgcgggtgcg agcacctcgg cgagcgtcgt ctccttcaac gggagattca gcgtgtaggg
     4681 aggcgattcg agcggctgct tcaagttctg cccgcccggc ttgtccttcg tgacaaactc
     4741 aaagttcaac cgcgccgggg tcttgccggt gaggatcgcg gcgcgtgatg ccgagcagat
     4801 cggcgcgggt gcataggcct gtgtgaagcg catgccctgg ctcgcgagtc ggtcgagatt
     4861 cggcgtgtga tggtagggat gcccgtagca ggcgaggtca ctccacgcga ggtcgtccga
     4921 gagaataaag acaatgttcg gcggtgcggc acccagaagc gccgaactgc acacgaggaa
     4981 tgtcaaaaca cgcgcgatca ctttttgcgg acggctttgg gtggagcgat cgggtaggcg
     5041 ggatcaggcg ttgatgcagt ggccagaatc tcctccatct gcgccaccac gtcgggatgc
     5101 ttcgcagcga cgtcctgtat ctccgtctga tcgtagcgga tgtcgtagag ttcgatgggc
     5161 tggtcggtgc cgttgcgcac ggccttccag tcgcccttgc gcgccgcctg cttgtactgg
     5221 ccgcggctgt ggccgtagtc ccagtagagg tagtcgtgcg gcggtgcctt gccgcctttc
     5281 agcgcggtga gaatcgagtg accgtcgatc gggtgcgtga tcttggcgcc gacgatctcg
     5341 gccagcgtcg gcatcacatc ccactgcgtc cagacttcgt cgctgacctt gccggcaggc
     5401 acgtgtcccg gccagcgcac cagcatcggc acgcggatgc cgccttcata cacatcgcgc
     5461 ttcacaccgc gcaacgctcc cgcgctgccg aagaacgccg gcttgtgctt cgcttcggcg
     5521 gaggggccgt tgtcgctcga gaaaaagatg agcgtgtcat ttgcgaggcc gctctcgtcc
     5581 agcaggtcca tgagacggcc gacatcgcgg tccagcatcg tcaccatcgc cgcgtaattc
     5641 ttttccacct gcggccagtc gcggtccgtg taaggcgcgt cgctgggaac gatttgcgat
     5701 tcgtccgact tcagcgggta atccgaccaa tgcggcagcg tggacgcata gaacaggaag
     5761 aacggctgtt cggcggaatc gcgaatgaac ttcagcgcac gttcggcaaa cagatcggcg
     5821 gtgtattgct gctttttctc accacgattt cccggcagca gcacgacgcg gtcgttgtcc
     5881 cagagatggt ccggataata gaagtgcgcc tgatcctgat cgacgtggcc gtagaagtca
     5941 tcaaagccct ggtcgttcgg tttgccctcc gagccatgta tgccgaggct ccatttgccc
     6001 accgcgcccg ttcgatagcc cgcctcatgc agcacctccg ccatcgtgat gtccgcattc
     6061 ttcaaaaagg tgccgtgcgg gacgttgtcg cggatgcgcg catggccgtt gtgcataccc
     6121 gtcatcaggc agcagcgcga cggagcgcag acgcacgacc cggcatacac ctgcgtgaaa
     6181 cgcgtcccgc ctgcggcaag ccggtcgaga ttcggcgtct ggatcaactt ctggccgtag
     6241 cagccgagct cgccgtaacc cagatcatcc gcgaggatga agatgatgtt gggcggtgcg
     6301 gcatgcgcag aaacaacaac cgacaggaag atgacaagca gacgagggat cacgggcaaa
     6361 atgaaggttc tggcacttga aggagccggc cagtggatcg aaaacgccac gcttcaaggc
     6421 aaccctgcac aaatcgcctt cgtcatcgca ggattctatt gtccatgcat gaactggcgt
     6481 gcggattctt gacgtgacct gccagatgcg gcggttcacg atccgtccgg aaacgactcg
     6541 tcggcacgtt gtggcttctc tcgctgctca aacgcccgga agtagttcag cacgggataa
     6601 tcgaaacggt tgccgagggc ttccttgagc ttgccgaggc tttccaggcc gtgctcagca
     6661 gcggcatctc ggatttcggc ttcctcctcg gctgtgaagt agtcgcgtgg ttcggccttc
     6721 agctcgccgt gctggatggc ctgggagagg tggttgtgga tggtgctggc gacgaacccg
     6781 cgaagcgaag cgattttttc gacgttgtgg ccctggcggt gcaggcgcag cgtttcgagc
     6841 gcggtgccag tgagtccgcc ctcgcttttc atcttcggcg gcggtggtgg cggcgtttcg
     6901 tcggcaaaac gcatcgcttc atgcgtttcg agccattcgg aaatcgcgcc catgaagacc
     6961 gcgccgtagt ccgcgagctt ctggctgccc acgccgggca aggcgaggaa gtcgcgttcg
     7021 gtgcgcgggt agcggcggca catgtggcgc agcgtcgtgt cgccaaacac gacgtagggc
     7081 ggcacaccgc gctcgtcggc cagttttttg cgcagcgcac gcagctcggc gaaaagcgcc
     7141 tcatcgcagg cgatgctgcc ggctttggcg actttgccgg tactcgtatc gcttttcacc
     7201 accacgggca cacgcgtgag catgaccggg gtgcggttca tgagcgtggc gaggccctgt
     7261 ttcgacaaag tgagcgtctg gaacgtgtcc ggcgaggcat caatgtaacc gagccgcatg
     7321 agctggcggc ccagcgcggc ccattcctca cgcggcgtgc ctttgccgat gccgtaagtg
     7381 ctaagctggt cgtgatacca tttccgaatc ttctccgtgt tcgctccgac gagcacctcg
     7441 acgacgtggt tgagcccggt gctgaatccg ctcttctgtt tgatgcggaa gatgcagctc
     7501 agcagctttt gcgcgtgcgt ggtggcgtcc caggtctcgc gcggttggag gcagatgtcg
     7561 cagccgccgc agttgtcgcc cggccactgc tcgccgaagt agtcgagcag cgccacgcgg
     7621 cggcattcgg cgccttcggc aaagtccgcc atcatgcgca tctgccgcgc ggcgatatcc
     7681 gcggccttgg cgtcggtcat ttcatcgagg aacttcaaat tgcgcaccaa gtcgccgcgc
     7741 gagtagagca gcacgcactc gctgggaagc ccgtcgcggc cagcgcggcc ggtttcttga
     7801 tagtagcctt cgatgttctt cggcaggtcg gcgtggatga catagcgcac gtccggtttg
     7861 ttgatgccca tgccaaaggc gatggtggca cagacgacac gagcctcatc acgaatgaag
     7921 gcctcctgat tggccgcccg ctcctccggc tggagtcccg cgtgatacgc caccgccttt
     7981 accccctccg ccgaaagcgc tgccgcgagg ctctccgcgc tttttcgcga ctgcacatac
     8041 acaatgcccg cctcctccgg tcgctggcgc acgaactcat acacttgccg tgttgatttg
     8101 gcctttggca ggatgctgta gctgagattg ggccggttaa agctcgccag aaagacctcc
     8161 ggttcatgca ggtgaagctg gcggatgatg tcgccccgca cctgctccgt cgccgtggcc
     8221 gtgagtgcga gaaacggcac gccgggcagc ctgtcacgta gcgtggcgag ctggcggtat
     8281 tccgggcgga aatcatgccc ccactcgctg atgcagtgcg cctcatcgac agcgatggcc
     8341 gtgacgttcc atcgctggag gtcgctgatg aagccgtcca tcatcacgcg ctccggcgcg
     8401 gcatagagca gcttgtagtg cccgttttcg aggcctgaac gccttcgatg tgcctcgctg
     8461 ccgtggatgg acgagttcaa aaacgtcgcc gccacgccac tcgccgtcag cgcatccacc
     8521 tgatccttca tcaacgcgat gagcggcgaa atgaccagcg tcagcccctc tcgcgccagc
     8581 gccggaagct gaaagcacag cgacttcccc gcgcccgtcg gcaaaatggc caccacatcc
     8641 cgattcgcga gcgaagcgtc cataatctcc ttttggagcg ggcggaagct gtcgtagccg
     8701 aagtgttcct tcagaagggt ggcgaggtcg ctggtcatgg gcatggattc gataaaccat
     8761 cgaatcctgt cctggaatgc ttcgattgcc agtcgaagcc gctgcttctg cgcgagcaaa
     8821 tttacgccgg cgaaatgaac atgcgcggac gtgtttgagc ggcctgggaa tgctcaccaa
     8881 cactgccgat gaaactcgga tcccgtttgc gccatgcgtc ttgtttcatg aatccggacc
     8941 gcgggccagg gcattcggat ccagtctgcg gatgatgaga agaactggcg gcccgtcaga
     9001 agctcttcaa acagcagcat tcatggattt gcagggggaa gcttgcacga ctgccaggcc
     9061 aggaagcgcc atttgccatc ctccaaacgc cagacggcga gataactgag gacggagtcc
     9121 atgacgccct tttcagacgc ggcctggatg cgcgcacgac cgctcaccag ggccacgccg
     9181 ggcgtgggga agctggtttt tctctccaca tggtcatact ccaggtagcg ggtgcggccg
     9241 gagacgagga tgtccacaaa cgaggacttt gtgtccacca ctccattcga gtgggcgtaa
     9301 tgcaggtcgt ccgaaaaaat ggcggagagc tttcccatgt cgggcgcctt catggcggcg
     9361 atgcgggcgt cgtcggcggc gaggattgcg gccacatcgg ccgcgtgaag ggaaaaaccg
     9421 aggatgcaaa gcagtgtgac gatgtgtttc atggatggtt ggatggtaat tcggggtcat
     9481 ttgcgcgacg cgcgggcccg ccattcatgg atgacggtga ctgcgagcgt cagcacggtc
     9541 agcggcacga caaaaaccat catcatcatg cggtagcccg gcagagactc gtgcttggtg
     9601 gcatcaaaaa agaggtacac gacataggcg atgtaataac cgagaaaaag caacccctcc
     9661 cagcggcgga tggtgcagcc gaaggtgaaa aagatgggca ggcaagccac ggcaacagcc
     9721 agcaccaccc agatgtcaaa attcagcgcc gcctcggaga caggcagtcc atcgggcgcg
     9781 gcgatggagc tcaggccgag gacgcagagg atgttgaaaa tgttgctgcc gaccacattg
     9841 ccgacggcga tgtcacgctc ccctttcaat gccgcgatca ccgatgtggc cacctccggc
     9901 agcgaggtgc ccgccgccac gatggtcagg ccgatcacca gctcgttcac tcccatggca
     9961 cgggccagca ttaccgcgcc ctccaccagc cagtgcgagc cgagcaccag catgccaaga
    10021 cccgccgcca caaaaaagag gttcgtcatg accgccttcg ccccgctgtc agcctggccg
    10081 aactcctcct caaaatcccc ctccttgtca tttttttcct tcccggcctg gagaaacagg
    10141 aaaacaacat acacgatcaa tccggacgta aaaatgacgc cgtccagacg gctgatcaac
    10201 cggtcccgcg ccagcagaag cagcagcgcc gagcaggcga tcatgatcgg cacatcgagc
    10261 cgcaccagct gcctggacac cgccagcggt gtgatgaccg ccgacaatcc aaggatgagc
    10321 agcacgttga acacattgct tccgagaacg ttccccaccg caaggtccgg ctgccccgcc
    10381 caggcggact gcacgctcac cgccagttcc ggcgaactgg tgccgaaagc caccacggtc
    10441 agccctatga ccagcggcgg aatgccaaag gccgccgcca gacgcgcggc gccgcgcact
    10501 agcaagtccg cgccgaaaat gaggagcgcg agaccgatga cgaacaaaac gagcgtgatt
    10561 aaatccatgc agccgcgtgc ttagccgtcc ccgccgcaag cgtcaagccc gcctccctct
    10621 ttgcgcgggc atggcctccc gcgcgtctca aactgaatgc ggtgtcagcc cgccttcatc
    10681 atcccgggga tttgcctcgt cgcgataccg atgggccggt ccagagggac gccggaacac
    10741 gcgagcaagg tggtgagcag atcgccttga ttgcctttca cggtgttgat gaagcggccg
    10801 gtgttgattg agccgccgcc tttgccggca atgatgaagg gcaggttctc gcgcgtgtgc
    10861 ttgttgccgt cctccaggcc gctgccccac agcatgatgc agttgtcgag aaggctcgtg
    10921 atcactgagg cgcggtgacc ttcagatgca caaagcgccg gccattactg ccagaaggga
    10981 tgctcgtctt cacctgctgc agggtgccgt cgtcggagag gacttgctgc gtgacgccag
    11041 ccgtatttct ggatgcgccc gggttctgct ctgcgcacca tttgtgtgcg caagccagcg
    11101 gactttagtt tgactccagc cggaagaagg cggcgttgtc cggcactgtg aactggaact
    11161 ccagcctgcc ctcaccgttg atgagcgtct gcgacgcact catgggaaac agcgtgaagc
    11221 tcgcgggcgc aagcgtggcg ctcttctcaa cgcccatcgt gagtgtgaac acgcccgtgg
    11281 ccggatgccg ctggaggagc ggcgcgccga cgtggagtgc ctgcacctgc gttgtggaga
    11341 agtagcccgc cgcgttcgca tttgtctccg ccttgccgag gttggaaaag agattcgtca
    11401 ccagcgcggg ctggctcacc tgccagttga agcccagcgc cgtgtaattg tgctccgccc
    11461 agtcgttcag cccgtcgccg tccgcatcgg cggtggtgga gtaggcgtcg cccgtgagcg
    11521 cgatgtcgaa aggattctcg ttgaggtcgc tgctcgccac atgcagcgcc gccgtgcgtg
    11581 tgctgctgat cgtgccggtg ggtgtgaaag tcacggtgaa ggtcgtgctg ccgccgatgg
    11641 cgacggtggt gctcatgcca ctcgagctca cgacatagtt gcccgagtgt gttccatcca
    11701 ccgtcacggc gatgcccgcg agcggcgcgg tgccggtgtt cttgatggtg aaagtcttcg
    11761 cgttgctgct accgatggcc accgcgccgt agccgatgct cgcgctgcca tccacgaggt
    11821 tcgtgcccgc cggctgctcc aatccgatct ccggcgtcac gcccgtgccg gtgagcgcga
    11881 tgtcgaaggg attctcatcc acgtcattgc tggcgatgtg gatcgccgcc gcgcgcgcgc
    11941 ccagcgcacc gggcgcgaag gtcaccgtga aggtcgtgct gccgcccgct gagaccggcg
    12001 tgctgatggc ggacaccgtg aagtccgccg cgttcgtccc gtccttggta atggcgagat
    12061 tgcgcagcgg cgtcgtgccc gtattacgca cggtgaaggt cttcaccgcg ttgctgtcca
    12121 gcagcacatc gccataaccc acactcgccg tgtcatccac gaggtccaac cccaccggct
    12181 cctctaccac gatctccgga tcaatgagcg tggtgaaggt catgtccatg ccgtagctgg
    12241 tgccgcccgc gttctgcgct acgatgcggt agtgatacag cgtgccgtcc agcagaccgg
    12301 tgacagcctg gttcaccgct atcgggttaa gcccggcacc ggggttttga gctgccgtgc
    12361 tcgtgccata actcgtggtc gtgccgtatt c
//
LOCUS       DAOMYJ010000158        12214 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Prosthecobacter sp. isolate AMR_MDS_5629
            NODE_1735_length_12214_cov_21.261699, whole genome shotgun
            sequence.
ACCESSION   DAOMYJ010000158 DAOMYJ010000000
VERSION     DAOMYJ010000158.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867850
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prosthecobacter sp. (wastewater metagenome)
  ORGANISM  Prosthecobacter sp.
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Verrucomicrobiaceae; Prosthecobacter.
REFERENCE   1  (bases 1 to 12214)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12214)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.30
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:34:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,742
            CDSs (total)                      :: 4,684
            Genes (coding)                    :: 4,676
            CDSs (with protein)               :: 4,676
            Genes (RNA)                       :: 58
            tRNAs                             :: 55
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12214
                     /organism="Prosthecobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1735_length_12214_cov_21.261699"
                     /isolate="AMR_MDS_5629"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1965333"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..3921
                     /gene="rpoC"
                     /locus_tag="PLP58_15265"
     CDS             <1..3921
                     /gene="rpoC"
                     /locus_tag="PLP58_15265"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012420081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HRJ09936.1"
                     /translation="DWECACGKYKRIKHKGVICDRCGVEVTLSRVRRERMGHIELAVP
                     VTHIWFYKCMPSRIGLMLDMTARSLERVIYYEDYMVIDPGGTPLQRGQLLTEVDLREA
                     EEQYGADAFRVGMGAEAIRDIFSQMNLAEMIQDLQDAMGKTRSKQTRKKLAKRLKLCQ
                     GFAESHTRPEAMIMEVLPVIPPDLRPLVPLEGGRFATSDLNDLYRRVINRNNRLKNLL
                     QLRTPDVIIRNEKRMLQEAVDALFDNGRHGRPVTGAGNRPLKSLSDMLKGKSGRFRQN
                     LLGKRVDYSGRSVIVIGPELGLHQCGLPKKMALVLFEPFIIRRLKEMGLVHTVRSAKK
                     MIERRTPEVWDILDEVTKGHPVLLNRAPTLHRLSIQAFEPKLIEGEAIRVHPLVCTAY
                     NADFDGDQMAVHVPLSVEAQMEARLLMLAPNNLFSPASGKPITTPSQDIPLGCFFLTY
                     LRDLPNHDNGKGRKGDHPDRLPIFNDPAEVEFALAESNLGLNDKILYRNPDFGHDGRH
                     FGDPSKPFISTTAGRVRFNEIWPDTLGFINATVGKKQISDIIWRTFQHVGQKETVACL
                     DRLKNLGFREATRSGCSIGITDMVIPDLKRATLDKTYKDIENVEKLYRKGLITNGERY
                     QQIVDLWTHATDQLADEVFRTLEYNDGKKHHNPLYVMVTSGARGNKTQIKQLAGMRGL
                     MAKPSGEIIERPITSNFREGLSVLEYFISSHGARKGLADTALKTADSGYMTRKLVDVS
                     QDVIVTEEDCGTVNGITLASIYQGDEEVVDLKTRVYGRTSCETIMDPVTKETIIAAGQ
                     LVLEKEAAHLSKIGVEKLKIRSVLTCESKRGCCAKCYGLSLATGRLVKIGEAVGIVAA
                     QSIGEPGTQLTMRTFHVGGAAMSSFKQPFINVKNGGKLRFSDMRTVQTPEGQWIALTK
                     NGILSIMDDDGKELESHKLVLGAIIAKPDGASVKKGEQVVTWDPYNMPIITEKAGKIE
                     FRDMISGITFTKEKNESTGNEEVVVIEHKEDLHPQIVVTDPKSHEVLASYTIPAGAHL
                     AVKNNEKVAAGTTLAKTPRKASKTKDITGGLPRVAELFEARKPKDACEIARIDGTVEI
                     GGLVRGKRRVIVTDQKTGQQEEHLIPRSKHILVFNGDHIHKGDQLTDGPVVPHEILEI
                     LGPQALREHLVNEVQEVYRAQGVEINDKHVEIIIRQMLRKVKITETGDTDFLWGDQVD
                     RTEFEKENERVIEEGGKPAEAEPVLLGITKASLETESFISAASFQDTTRVLTEAATLN
                     KSDFLRGFKENVIMGHLIPAGTGFIANRSFDLAEPGRQADEEAA"
     gene            4023..4583
                     /locus_tag="PLP58_15270"
     CDS             4023..4583
                     /locus_tag="PLP58_15270"
                     /inference="COORDINATES: protein motif:HMM:NF014659.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgE/AlgH family protein"
                     /protein_id="HRJ09937.1"
                     /translation="MTPSSDEQAPHLSGTLLLAVPSMRDPNFQRAVVFIAAHTREDGA
                     FGYVLNRPLAQRVADLLPDQDLGALGQVPVFVGGPVAADKLAFASLHWNRKKGTLRCQ
                     THLSVSDALHEMSMGHEVRGFVGYSGWSSGQIENEIQHRSWIITPARRVILATEKPEA
                     MWGAVLDDMGPVFSLMARTPENVRLN"
     gene            4635..6887
                     /locus_tag="PLP58_15275"
     CDS             4635..6887
                     /locus_tag="PLP58_15275"
                     /inference="COORDINATES: protein motif:HMM:NF024907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VCBS repeat-containing protein"
                     /protein_id="HRJ09938.1"
                     /translation="MRFLPLILSVPLMAAAEGLYFDGPEVVKLDWNTTALRSADFNGD
                     GLPDLALINRDRARIEFLFQRKDGPKAGEPERTSREDRWNPVLELSRLDKKPLVIGRR
                     AHALAVGDWNGDGRPDIAFTTDEKKLFLRIQAAAGDWTSNTEFTLDSVSEDPESLHAA
                     DLNGDGRTDLALLTETRLHVWLQSASGAWSDGKTYALGQNGCGGLRQADLDGDGLTDL
                     CYTSPDADAVFVRLQQKGCVFGQEWRLQIPESRCWVHPFRFGKEAGLAWIRDDTGMIE
                     AARLARTDADTDADRASSIRHAIPATDIKAGAAVYGDLDGDGRPDVVIAEAKNARLWF
                     FAGLPGGAFAEGREFPALSGIESLAIAVISGKPALLMLSPAEKSVGVSRWQKNRLTYP
                     DVIHQSADTLLALAAGSIDGEKSAAVHALAESKGKVSLLSLAWSEQDKSFKQVVRDLP
                     SAPGKPSALRIFDADQDGRGDLFIFSTLAPMQVLLSREDEKTPFKRAEGLPDNLLNKL
                     APAAFTTADLDGDGKAEFLIARDQLARAFRIGQDGKARTVEQFNAPDSSAQISTLAAA
                     AGGGVFLADTASGKLYEMTPGPDGVFRAGHTHALAGTAPEECHLLSLPDQGSALLLVG
                     KNGFDINPFTGPTLRLQTLATFDSGLKDTAAADLLIAPFSGAAVDDILALDNGKSRVI
                     ELFTPAAHSPPSWSSSLYFRVFDIDPQYRGKVGLTNEPHDYLAPDLDGDGRPDLALLV
                     HDRLLLYMRK"
     gene            complement(6891..7427)
                     /locus_tag="PLP58_15280"
     CDS             complement(6891..7427)
                     /locus_tag="PLP58_15280"
                     /EC_number="1.5.1.3"
                     /inference="COORDINATES: protein motif:HMM:NF012413.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0046654 - tetrahydrofolate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HRJ09939.1"
                     /translation="MVEVTLIAAVSADGFISCGRGIPWDLPEDKKHFRAYAEKKWLLL
                     GRVTHSEMSGWFRDHHPLVMTRDAGCKVTPGQCVASVEEALHLAESADQPELLVCGGS
                     QIYAEAMPFADKLVITHVDRILGAGLPFPKIHRQSWEPVSRIIHGTDALHDISFEIIT
                     WRRVAALPGAGWTVGEAA"
     gene            complement(7440..8258)
                     /locus_tag="PLP58_15285"
     CDS             complement(7440..8258)
                     /locus_tag="PLP58_15285"
                     /inference="COORDINATES: protein motif:HMM:NF024874.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HRJ09940.1"
                     /translation="MLIPTLLSPRRPGRTPRAVPLASNDEICATWLGHAGFFASIGGV
                     SMLIDPNWALWHGPVKRVRHPSIWASDLPPIDLVLITHAHFDHLHLPSLRRVARGQPV
                     IVPHGVGDIVKRCGFGQIIELETWQRAKFQDIHITFTPARHWGARMIHDTHRGFGGYL
                     VSSGRRCLYHCGDSSMFDGFQEIGRRADIDLAIMPIGAYQAPSGRPVHMNPAEALDAF
                     EMLGARHMIPMHHDTFPLGGEPIHEPADWLSLLSTERRVSERVRILHEGESVVY"
     gene            8521..9120
                     /locus_tag="PLP58_15290"
     CDS             8521..9120
                     /locus_tag="PLP58_15290"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR02727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030272 - 5-formyltetrahydrofolate
                     cyclo-ligase activity [Evidence IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HRJ09941.1"
                     /translation="MNQDNSTWTKTDLRRSMRARLAAVDENQLAEWSARLVKRLQMRR
                     EIWEKPGTAALFGGLRAEPDLVSTLMPWLAGQGWQTVFFRISEGMLEPRVVRDMGDLR
                     RGRMGVWEPSMQTSPVPVGELTVILVPGLMFSTKDGSRLGHGGGYYDRLLSQQGVRAP
                     RLGVAFDAQLAENLPCEPHDMRVQSLATESRFVESFPDG"
     gene            complement(9158..9370)
                     /locus_tag="PLP58_15295"
     CDS             complement(9158..9370)
                     /locus_tag="PLP58_15295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ09942.1"
                     /translation="MTRHESIQMIRDACKTVALQFMKIHPALPHLRDSETMSDCLKAV
                     HEMTVQLEIIKKKIGRLEKQDDSSLV"
     gene            9530..10363
                     /locus_tag="PLP58_15300"
     CDS             9530..10363
                     /locus_tag="PLP58_15300"
                     /EC_number="1.14.19.-"
                     /inference="COORDINATES: protein motif:HMM:NF012699.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016717 - oxidoreductase activity, acting
                     on paired donors, with oxidation of a pair of donors
                     resulting in the reduction of molecular oxygen to two
                     molecules of water [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA desaturase"
                     /protein_id="HRJ09943.1"
                     /translation="MTHPAFKPTAGAFLFFGIHLLAILAFFVKPDPLSLCLCAGLFLI
                     RKFGITGGYHRYFSHRSFKTSRWFQFVLAWLGGMAAQKGALWWAAHHRHHHQHSDQPD
                     DIHSVKQQGFYWAHVGWILAPDYEDYDAKRVKDLTKYPELVWINTYHWVPPAVLALVC
                     YLAAGLPGFLWGFCLSTVVLYHTTFAINSFCHLLGNRRYETGESSRNSFILAILTLGE
                     GWHNNHHYYPHCARQGFFWWEFDPTYYVLRLLKLAGIVHAIKEPTKRVLEGREATAEL
                     G"
     gene            10432..11562
                     /locus_tag="PLP58_15305"
     CDS             10432..11562
                     /locus_tag="PLP58_15305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009958449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ09944.1"
                     /translation="MPDLISDPKDLKPTTAWQETVWAWTAEEELADHTSKARLCVATL
                     LPFKDRKPDWEGFSKSVRWMRQCGEHYGVEMVFVLNADTGYIFELDNQLYADVLRRFR
                     AEFPDQRFIAGVTARGTEKDEVFKAERYWPLLDIAQSHDNCEVMIMTSRLLNLLETEA
                     RRDAYFEIAAHITHPAIAHALEPSFVPWASPYEPWLLHEIANHPKYAGGKISTLDEPH
                     FLYWAAMCKDLKLPFAPHSGDDYGIPTAIRLGLPLLIGAGVSACPLICAARDMWLLDS
                     VADKKFKTGTGRFDTRVYKLFEAFQSFEDLVFRLDERMSAASYKHSTAHVLSNLGIID
                     APEPHPECKDVRGMDEALRMAEALRRPLRMAKRLGIPNFEAV"
     gene            11701..12006
                     /locus_tag="PLP58_15310"
     CDS             11701..12006
                     /locus_tag="PLP58_15310"
                     /inference="COORDINATES: protein motif:HMM:NF024833.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper domain-containing protein"
                     /protein_id="HRJ09945.1"
                     /translation="MTPYPGPAEVQGGVTGAVVGGLAGAVIGHQSGCPLEGAAIGGAL
                     GALAGSAIGASEDARYGYHPRYAPVRPQRFHVAPPVILAPAPIFGGVGYSRWGGRGF"
BASE COUNT         2393 a         3865 c         3551 g         2405 t
ORIGIN      
        1 gactgggagt gcgcctgcgg caagtacaag cgcatcaagc acaagggcgt gatttgcgac
       61 cgctgcggtg tcgaggtcac cctctcccgc gtgcgccgcg agcgcatggg ccacattgag
      121 ctggccgtgc cggtcactca catctggttc tacaaatgca tgccctcacg catcggactc
      181 atgctcgaca tgacggcccg ctcgctggag cgcgtcattt actatgagga ctacatggtc
      241 attgatcccg gcggcacccc gctccagcga ggccagctcc tcaccgaggt ggacctgcgc
      301 gaggccgagg aacaatacgg cgccgacgcc tttcgcgttg gcatgggcgc cgaggccatc
      361 cgcgacattt tcagccagat gaatctggcc gaaatgatcc aggatcttca ggatgccatg
      421 ggcaagaccc gctccaagca gacccgcaaa aaactcgcca aacgcctcaa actctgccag
      481 ggctttgccg aaagccacac gcggcccgag gccatgatca tggaggtgct gcccgtgatc
      541 cccccggact tgcgtccgct cgtcccgctc gaaggcggcc gcttcgccac ctccgacctg
      601 aacgacctat accgccgcgt catcaatcgc aacaaccgcc tcaaaaacct gctccagctc
      661 cgcacgccgg atgtcatcat ccgcaatgaa aagcgcatgc tgcaggaggc ggttgatgct
      721 ctcttcgaca acggccgtca tggtcggccc gtcaccggcg caggcaaccg cccgctcaaa
      781 tccctctccg acatgctcaa gggcaagtca gggcgcttcc gccagaacct gcttggcaag
      841 cgcgtggact attccggacg ctctgtcatc gtcattggcc ccgagttggg cctgcatcaa
      901 tgcggtcttc cgaaaaagat ggccctggtg cttttcgaac ccttcatcat ccgccgcttg
      961 aaggagatgg gtctcgttca cactgtccgt tccgccaaaa agatgatcga gcgccgcact
     1021 ccggaagtgt gggatatcct cgacgaggtg accaagggcc acccagtgct gcttaaccgc
     1081 gccccgacgc ttcaccggct ctccatccag gcattcgagc ccaagctcat cgaaggcgag
     1141 gccatccgtg tgcatccgct ggtctgcacg gcttacaatg ccgacttcga cggagaccag
     1201 atggccgtcc atgtgcccct ctctgtggaa gcccagatgg aggcccgtct gctcatgctg
     1261 gcgccgaaca atctgtttag ccccgccagc ggcaagccaa tcaccacccc ttcccaggac
     1321 attcccctcg gctgcttctt cctcacctat ctgcgtgatc tgccgaacca cgacaacggc
     1381 aagggcagga agggcgacca cccggaccgc ctgcccatct tcaatgatcc ggccgaggtt
     1441 gagtttgcgc ttgccgagag caatctcggt cttaatgaca aaatcctcta tcgcaacccg
     1501 gacttcggtc atgatggccg ccattttggc gacccatcga agcccttcat ctccaccacc
     1561 gcgggtcgcg tccgtttcaa tgagatctgg cctgacaccc ttggcttcat caatgccacc
     1621 gtcggcaaga agcagatctc cgacatcatc tggcgcacct tccagcatgt cgggcagaaa
     1681 gaaaccgttg cctgcctgga ccgcctgaaa aatctcggtt tccgcgaggc gacccgctcc
     1741 ggctgctcca tcgggattac cgacatggtc atccccgacc tcaagcgcgc gaccctggac
     1801 aagacctaca aggacatcga aaacgtcgaa aaactttacc gcaagggact catcaccaat
     1861 ggcgagcgct atcagcagat tgtggacttg tggacgcatg ccaccgacca gctcgccgac
     1921 gaagtgttcc gcaccctgga atacaacgac ggcaaaaagc accacaatcc gctctatgtc
     1981 atggtcacct ccggtgcccg cggcaacaag acgcaaatca aacagctcgc cgggatgcgt
     2041 ggcctgatgg ccaaaccctc cggtgagatc attgagcgcc ccatcacttc gaatttccgc
     2101 gaggggctca gcgtgctgga atacttcatt tcctcacatg gcgcccgcaa ggggctggct
     2161 gacactgctt tgaagactgc ggactccggc tacatgaccc gcaagcttgt ggatgtgtcc
     2221 caggatgtca tcgtcacgga agaggactgc ggcacggtca acggcattac gctcgcctcc
     2281 atctatcaag gggatgagga ggtggtggat ctcaagaccc gcgtctatgg acgcacgagc
     2341 tgcgagacca tcatggaccc cgtcaccaag gagaccatca ttgctgccgg ccagcttgtg
     2401 ctggagaagg aggccgccca tctgtccaag atcggcgtcg aaaaattgaa aattcgctct
     2461 gttctcacct gcgagagcaa gcgcggctgc tgcgccaaat gctacggtct cagcctggcc
     2521 actggcaggc tggtgaagat tggcgaggcg gtcggcattg tcgccgcgca gtccatcggc
     2581 gagccgggca cccagctcac catgcgcacc ttccatgtcg gcggcgctgc catgagcagc
     2641 ttcaagcagc ccttcatcaa cgtgaaaaat gggggcaagc tccgcttctc ggacatgcgc
     2701 accgtgcaga ccccggaagg ccagtggatc gccctcacca agaatggcat tctttccatc
     2761 atggatgacg acggcaagga gctggagagc cacaagctcg tgctcggcgc catcatcgcc
     2821 aagccggacg gcgccagcgt gaagaagggc gagcaggtcg tgacctggga tccctacaac
     2881 atgccgatca tcaccgaaaa agcgggcaag attgagttcc gcgacatgat ctccggcatc
     2941 accttcacca aggagaaaaa cgagtccaca ggcaacgaag aggtcgtcgt catcgagcac
     3001 aaggaggacc tgcatccgca gatcgttgtc accgacccga agagccacga ggtgctcgcc
     3061 agctacacca ttcccgccgg cgcccacctt gccgtcaaaa acaacgaaaa agtcgcggcc
     3121 ggcaccaccc tggccaagac accgcgcaag gcgtccaaga ccaaggacat caccggcggt
     3181 ctgccgcgtg tggccgagct tttcgaagcc cgcaaaccca aggatgcctg cgagattgcc
     3241 cgtattgacg gcaccgtcga aatcggcgga ctcgtccgcg gcaagcgccg cgtcattgtc
     3301 accgaccaaa aaaccggcca gcaggaggag cacctcattc cccgctccaa gcacatcctt
     3361 gtcttcaacg gcgaccacat tcacaagggc gaccagctca ccgacgggcc cgtcgtgccg
     3421 cacgagatcc tggagatcct cggtccgcag gctctgcgcg agcaccttgt caacgaggtc
     3481 caggaggttt accgagccca gggtgtggag atcaacgaca agcatgtcga gatcatcatc
     3541 cgtcagatgc tgcgcaaggt gaagatcacc gaaaccggcg acaccgactt cctctggggc
     3601 gaccaggtgg accgcaccga gttcgagaag gaaaatgagc gagtcatcga ggagggcggc
     3661 aagcccgccg aggccgagcc tgtgctcctt ggcatcacca aggcttccct tgagacggag
     3721 tccttcatct ccgcagcgtc cttccaggac accacccgcg tgctcaccga ggcggccacc
     3781 ctgaacaagt ccgacttcct ccgcggattc aaggagaacg tcatcatggg ccatctcatc
     3841 cccgcgggca caggcttcat cgccaaccgc agcttcgacc ttgccgagcc aggccgtcag
     3901 gcggacgagg aagccgcctg aggtcagtcc cgccatcctg tgaaaacccc gcctgcatcc
     3961 tgccggcggg gttttttctt gccaagggaa cattggaggc gcatcccttg tcagagtttg
     4021 acatgactcc ttcgtctgat gaacaagctc cgcacctctc cggcaccctg cttctcgcgg
     4081 tgccctcaat gagagacccc aattttcaac gcgccgtggt cttcattgcg gcgcacactc
     4141 gtgaggacgg cgcgttcggc tatgttttga accgccccct tgcccagcgt gtggccgacc
     4201 tcctgcccga ccaggatctc ggcgcgctcg gccaggtgcc cgtcttcgtc ggcggccccg
     4261 tcgccgcgga caagcttgcc tttgcctctc tgcattggaa tcgaaaaaaa ggcaccctcc
     4321 gttgccagac ccacctttcc gtttccgacg cccttcatga gatgagcatg ggccacgaag
     4381 tgcgcggctt cgttggctac tccggctggt ccagcggaca gattgaaaac gaaattcagc
     4441 accgctcctg gatcatcact ccggcgcgca gggtcatcct tgccacggaa aagcccgagg
     4501 ccatgtgggg cgcagtgctt gatgacatgg ggcccgtttt cagcctcatg gcccggacgc
     4561 cggagaatgt gaggctgaat tgagcggatg ttccttcaag gttgcatcca tgcgcggggc
     4621 gctgagattc gcagatgcgc ttcctgcccc tcattctctc cgtccctctc atggcggccg
     4681 ccgaaggtct ttatttcgac ggcccggagg tggtgaaact ggactggaac accacggctc
     4741 tgcgctccgc tgacttcaat ggcgacggac tgcccgacct cgccctcatc aaccgggacc
     4801 gggcccgcat cgagtttcta ttccagcgaa aagacggccc caaggccggc gaacccgagc
     4861 gcacttcgcg cgaggaccgt tggaaccccg tgctggaatt gtcacgcctc gacaaaaagc
     4921 ccctcgtcat tggccgccgc gcccatgccc tcgctgtggg agattggaac ggggacggcc
     4981 ggccggacat cgcctttacc actgacgaga agaaactctt ccttcgcatc caggctgcgg
     5041 cgggtgactg gacttcgaac acggaattca ccctcgactc cgtgtccgag gatcccgaat
     5101 ccctgcacgc tgctgatctc aatggcgatg gacgcacgga tttggcgctg ctcaccgaga
     5161 cacgtctgca tgtctggctc cagtctgcat ccggcgcttg gtcggatgga aaaacttacg
     5221 ccctgggcca gaacggttgc gggggcctgc gccaggctga cctggatggc gatggtctga
     5281 ccgatctttg ctacacgtct cctgatgctg acgccgtctt tgtcaggctt cagcagaaag
     5341 gctgcgtctt cgggcaggag tggcggctgc aaattcccga aagccgctgc tgggttcatc
     5401 ctttccgttt tggcaaagaa gccggcctcg catggatccg cgatgacacc ggcatgatcg
     5461 aggccgcccg ccttgcgcgc accgatgctg acacggatgc cgaccgcgcg tcgagcatcc
     5521 gccatgccat tcccgccacc gacatcaaag caggagccgc tgtttatggc gacctggatg
     5581 gcgatggccg cccggatgtc gtcatagccg aggcaaaaaa cgcccgcctc tggttcttcg
     5641 ccggcttgcc cggcggggct tttgccgaag ggcgcgagtt tccggcgctc agcggcattg
     5701 agagcctcgc catcgcagtc atctccggca agcccgccct cctcatgctc agtcctgcgg
     5761 agaaaagcgt tggcgtctcc cggtggcaaa aaaaccggct cacctacccc gatgtcatcc
     5821 accagagcgc cgacacactg ctcgctctcg cagccggttc cattgacggg gaaaagagcg
     5881 ccgccgttca tgctcttgcg gaatccaaag gcaaagtttc tttgctcagc ctggcttgga
     5941 gcgagcagga caaatccttc aagcaggtcg tccgcgatct gccgagcgcg ccgggcaagc
     6001 cctccgccct gcgcatcttc gatgccgacc aggatggcag gggtgatctt ttcattttct
     6061 ccaccctggc tcccatgcag gtgcttctct cccgcgagga tgaaaaaacc ccgttcaaac
     6121 gcgccgaggg cctgccggat aatctgctca ataaactggc tcccgccgcc ttcaccaccg
     6181 ccgatctgga cggcgatggc aaagccgagt ttctcatcgc gcgcgaccag cttgcccgcg
     6241 ccttccgcat cggtcaggat ggcaaggcgc gcaccgtcga gcagttcaac gcgccagatt
     6301 catccgccca gatcagcact cttgccgctg cggcaggcgg cggggtattt ctcgccgaca
     6361 ccgcttctgg caagctctat gagatgaccc ccggccctga cggcgtgttc cgcgcagggc
     6421 acacccacgc gctggccgga accgcgccgg aggaatgcca cctgctctcc ctgccggatc
     6481 aaggctcggc tctgctgctt gtcggcaaaa acggctttga catcaacccc ttcaccggtc
     6541 ccacgctccg tttgcagacc cttgccacct ttgacagcgg cttgaaggac accgccgccg
     6601 cggatcttct cattgcgcct ttcagcggcg ctgccgtgga tgacatcctt gcgctcgaca
     6661 acggaaagag ccgcgtcatc gagttgttca ctcccgccgc tcactctcct ccttcctgga
     6721 gcagctcgct gtatttccgc gtcttcgaca ttgaccctca ataccgcgga aaagtcggtc
     6781 tcaccaacga gccgcacgac tacctcgccc ccgacctcga cggcgacggg cgccccgacc
     6841 tcgcactcct cgtgcatgac cggctgctgc tttacatgag gaaatgaggt tcatgccgcc
     6901 tcgccgactg tccagccggc tcccggcaaa gcggcaacac gccgccaggt gatgatctca
     6961 aaggagatgt cgtgaagcgc gtccgtgccg tgtattatgc gtgacacagg ctcccaactc
     7021 tggcggtgga tttttggaaa aggcaggccc gcgccgagaa tgcgatccac atgcgtgatc
     7081 acgagcttgt ccgcgaaggg catggcttca gcgtaaattt gagatccgcc gcacacaagg
     7141 agttcgggct ggtcggcact ttcggcgaga tgcaaagcct cctcaacaga cgccacacac
     7201 tggcctggtg tgactttgca tcccgcgtcg cgcgtcatca ccagcggatg atgatcgcgg
     7261 aaccagccgc tcatttctga atgtgtgact cgtcccagca gcagccattt tttttcggcg
     7321 taggcacgga agtgtttttt gtcctccggc agatcccagg gaatgccgcg tccgcatgag
     7381 atgaaaccat ctgcggagac tgcggcgatg agtgtgactt ccaccatgcc gccatccctt
     7441 tagtacacca cggactcgcc ttcgtgaagg atgcgcaccc gttccgagac gcggcgctcc
     7501 gtgctgagga gtgacagcca gtcggcgggt tcgtggatcg gctctccgcc cagagggaag
     7561 gtgtcatgat gcatcgggat catgtgccgc gcgccgagca tctcaaaggc gtcgagagcc
     7621 tcggccgggt tcatgtgcac cgggcgcccg ctcggtgcct ggtaggcgcc gatgggcatg
     7681 atggccaggt caatgtcggc gcggcggccg atctcttgaa aaccgtcgaa catgctgctg
     7741 tccccgcagt ggtagaggca gcgtcggccg gaactcacca agtagccgcc aaaccctcga
     7801 tgagtgtcat gaatcatgcg ggcaccccag tgacgcgccg gtgtgaacgt gatgtggatg
     7861 tcctgaaatt tggcccgctg ccaggtttcc agttcgatga tctggccgaa tccgcatctc
     7921 ttcacgatgt caccgactcc atgtggaacg atgaccggct ggccgcgggc cacgcgcctg
     7981 agacttggca gatgcagatg gtcgaagtga gcatgggtga tgagcaccag gtcaatcggc
     8041 ggcagatcac tggcccagat gctcggatgg cgcacccgtt tgacggggcc gtgccacagc
     8101 gcccagttcg gatcaatcag catgctcact ccgcctatgc tggcgaaaaa gcccgcgtgc
     8161 cccagccagg tggcgcagat ttcgtcgttg gaggccagag gcacggcgcg cggagtcctt
     8221 cccggacggc gcggtgacag cagcgtgggt atgagcacat ctccgaggaa ttgaagattc
     8281 ctgcgttgcc agccctcggt cgggagaagt ccgattttat tttcgtccct ggtcttcctg
     8341 cgaaagggaa aaccggcttt tccgggcctg cacaaaaggg cgggtccgcc atggtccggc
     8401 gtgggatcgt gaatcatgag tttgaactcg ttctaatgaa aaatagggct gtttgtcatg
     8461 gagacaaggc ggaagtccgg ctttgcaagg cacgatccaa gagtgacaat tcacgcccgc
     8521 atgaaccagg acaactccac ttggaccaaa accgacctgc gccgttcaat gcgcgcgcgc
     8581 ctcgcggcgg tggatgagaa tcaactggcg gagtggtccg caagattggt gaagcgtctg
     8641 cagatgcggc gcgagatttg ggaaaagccc ggcacggctg cattgttcgg tggactgcga
     8701 gccgaacctg acttggtgag caccttgatg ccgtggcttg ccggacaggg atggcaaacg
     8761 gtcttttttc gcatttcgga gggaatgctg gagccgcgtg tggttcgcga tatgggagat
     8821 cttcggcgcg gcaggatggg ggtttgggag ccttcgatgc aaacctcccc cgttccggtg
     8881 ggggaattga ccgtgattct tgtgccggga ttgatgtttt ccacaaaaga cggctcccgt
     8941 ctgggacatg gcggcggcta ttatgaccgg ctgctttcac agcagggagt cagggctccg
     9001 cgcttgggtg tcgcattcga tgcgcagttg gcggaaaatc tgccctgcga gccgcatgac
     9061 atgcgcgtac agtctttggc gaccgagtca cgatttgtcg agagtttccc tgatggctga
     9121 caggtgtgcg aatgcgggac ccgcccgcgc gtgtcattca aacaaggctg ctgtcatcct
     9181 gcttttccag cctgccgatc ttctttttga tgatctcgag ttgaaccgtc atttcatgca
     9241 cggctttaag gcagtccgac atagtctcgg aatcccgcag atgcgggagt gctggatgaa
     9301 tcttcatgaa ttgcagcgcc acggttttgc aggcatctcg gatcatctgg atggactcat
     9361 gacgggtcat gacccgacaa tgaacacatg ccgccggaag gcaagcccgg cttcccggag
     9421 gacgggaggg ctattgtgcc aatctcgtgc ctttcaaaac gcgcggttgc tattttcatg
     9481 attcaggctg taatccggcg ctctcccacc aaaatcagca gtcagttttg tgacccaccc
     9541 agccttcaag cccacagccg gtgccttcct ctttttcggc atccacctcc tcgcgatcct
     9601 cgccttcttc gtcaaaccgg acccgctctc gctatgtttg tgcgcggggc tgtttttgat
     9661 ccggaagttc ggcatcaccg gcggttatca ccgctacttt tcacaccgct cgttcaaaac
     9721 ctcgcgctgg tttcaatttg tcctggcctg gctgggcggc atggcggcgc aaaaaggcgc
     9781 gctctggtgg gcggctcatc accgtcatca ccaccagcac tccgaccagc ctgatgacat
     9841 tcattccgtg aagcagcagg gcttttattg ggcgcatgtg ggctggattt tggcgccgga
     9901 ttatgaggac tatgacgcga aacgcgtgaa ggacctgacc aagtatccgg agttggtgtg
     9961 gatcaacacc taccattggg tgcctccggc ggtgctggcg ctggtctgct atctggctgc
    10021 cgggctgccg ggcttcttgt ggggcttctg cctgagcacg gtggttcttt accacaccac
    10081 cttcgccata aattccttct gccatctgct tggcaaccga cgttacgaga cgggcgagtc
    10141 gagccggaac tccttcatcc ttgccattct cacgctcggc gagggctggc acaataacca
    10201 ccactactac ccgcattgcg cgcgccaggg ctttttctgg tgggagttcg acccgaccta
    10261 ttatgtgctt cgcttgctga agcttgcggg cattgtccac gccatcaaag agccgacgaa
    10321 gcgcgtgctg gaaggcaggg aagccacggc ggaactcggt tgagagccgg cccattattc
    10381 tccggcaaag gtcgtttgcc atgaggggat gtccgtctag ccttggcggc catgcctgac
    10441 cttatctccg acccaaaaga cctgaaacct acgacagcct ggcaggaaac tgtctgggcc
    10501 tggaccgccg aggaggaact ggcggaccac accagcaagg cgcgcctgtg cgtggcgacg
    10561 ctgcttccct tcaaggaccg caagccggac tgggagggtt tttccaaaag cgtgcgctgg
    10621 atgcggcagt gcggcgagca ctatggggta gagatggtct tcgtgttgaa cgcggacact
    10681 ggatacatct tcgaactgga caaccaactt tatgccgatg ttctgcgccg gttcagggcg
    10741 gaatttccag atcagcgatt catcgcaggc gtgacagcgc gcggcacgga gaaggatgag
    10801 gtcttcaagg ctgagcgcta ctggcctctg ctcgacatcg cccaaagcca cgacaactgc
    10861 gaggtgatga taatgacttc ccgcttgctg aacttgctgg agaccgaggc gcggcgggat
    10921 gcctacttcg agatcgccgc tcatatcacg cacccggcca tcgcccacgc tttggagccc
    10981 tcttttgtgc catgggccag cccgtacgag ccgtggctgc tgcacgagat cgccaaccac
    11041 ccgaaatatg cgggcggcaa gatcagcaca ctcgacgagc cgcatttcct ctactgggcg
    11101 gccatgtgca aggatctgaa gttgcctttc gccccgcaca gtggtgacga ctacggcatt
    11161 cccacagcca tccggcttgg ccttccgctg ctcatcggcg cgggtgtcag cgcctgcccg
    11221 ctcatctgcg ccgcgaggga catgtggctg ctggacagtg tggcggacaa gaaattcaaa
    11281 acaggcacgg gccgctttga cacgcgggtt tacaagctct tcgaggcatt ccaatctttt
    11341 gaagacctcg tcttccgcct cgacgaacgc atgagcgccg cgtcttacaa gcacagcacg
    11401 gcccatgtgc tgagtaatct gggcatcatt gatgcgccgg agccgcatcc tgaatgcaag
    11461 gatgtgcgcg gcatggacga ggcgctgcgc atggccgagg cgctgcggcg cccgttgcgc
    11521 atggcgaagc ggttgggcat tccaaatttc gaggctgttt gaatgatggc aaatgaagcg
    11581 cggctcaaga gaccagcccc tcattttatg aagccgcgac ttccttccgc tctcaaaata
    11641 tccatcttcc gcgcgctgtc cgctttgctg gctgggggcg cgcttttttt aacctcatgc
    11701 atgacgcctt atcccggtcc cgccgaagtt cagggaggcg taactggcgc ggtggttgga
    11761 gggcttgccg gcgcggttat cggccatcaa agtggatgtc ccctggaggg cgcggccata
    11821 ggcggcgcgc tcggagcgct cgcgggatcg gctattggcg caagcgagga cgcccgctat
    11881 ggctaccacc cccgctacgc acccgtcagg ccacagcgtt tccatgttgc tccaccagtg
    11941 attttggcac ccgcgccgat ctttggcggt gtgggctaca gccgctgggg cggacgcggt
    12001 ttttaatccg gtggtgttgg ttttggcggc cctgccttcg gtggaccagt gtattcgcct
    12061 ggatgggggt atccttgctg gtgacaccct ccatcaattc ggccaacgat cacaaatgca
    12121 tccagcggta ttgcgaccat tttaaaatta tcgttgagca tctgcgagtt gagcaggagt
    12181 tgcgttttac gggcgagggg ggaggcgggg aaaa
//
LOCUS       DAOMYJ010000159        12212 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Prosthecobacter sp. isolate AMR_MDS_5629
            NODE_1736_length_12212_cov_21.178169, whole genome shotgun
            sequence.
ACCESSION   DAOMYJ010000159 DAOMYJ010000000
VERSION     DAOMYJ010000159.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867850
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prosthecobacter sp. (wastewater metagenome)
  ORGANISM  Prosthecobacter sp.
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Verrucomicrobiaceae; Prosthecobacter.
REFERENCE   1  (bases 1 to 12212)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12212)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.30
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:34:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,742
            CDSs (total)                      :: 4,684
            Genes (coding)                    :: 4,676
            CDSs (with protein)               :: 4,676
            Genes (RNA)                       :: 58
            tRNAs                             :: 55
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12212
                     /organism="Prosthecobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1736_length_12212_cov_21.178169"
                     /isolate="AMR_MDS_5629"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1965333"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            173..931
                     /locus_tag="PLP58_15315"
     CDS             173..931
                     /locus_tag="PLP58_15315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009962309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="platelet-activating factor acetylhydrolase IB
                     subunit"
                     /protein_id="HRJ09946.1"
                     /translation="MKLPTLSLLALSFLLASCARERVIPVHVATTPVAEPNEAKMKRH
                     ESFNEVSKQGEATLVFLGDSITQGWEGKGKAAWEKYYASRKAANFGIGGDRTEHVLWR
                     LENGNFDGLKPKLIVLMIGTNNTGHQNRPMPEINNQIYTCSAEQTADGVELILRKLRK
                     KAPFADILLLGIFPRGETPEDPMRKQNEATNVLISKLADGKTIHFMDIGKTFLQSDGT
                     LTKEIMPDLLHLSEKGYEMWAGAIEPKIKELLGE"
     gene            1095..2273
                     /locus_tag="PLP58_15320"
     CDS             1095..2273
                     /locus_tag="PLP58_15320"
                     /inference="COORDINATES: protein motif:HMM:NF023327.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BtaA family protein"
                     /protein_id="HRJ09947.1"
                     /translation="MPVTLKSRTPLRHLAQDVIFNRVHGGNLVYNTCWEDPRPDLEML
                     DLRADSRLVMITSAGCNALDYLLAAPARIHAVDMNPRQNALLQLKLAMIQRGDFEDFF
                     ALFGRGAHPRFREILADLDLPPYAARYWRAKHYYFQSTPLNPSFYYRGTAGQMAWITL
                     QSFTRGSRKVRGYIHALLETGTLEEQRRLYDEVKPDLWSGLVSWLLRQPVSMTMLGVP
                     RAQVRLIEANFPGGILGYIRQKLEHVLTETLFLENYFWRVYLTGRYTRECRPRYLREE
                     HQPLLRERAERIATHSTTVANFLREHPGAYSHFVLLDHQDWLAAHRPQALEEEWRLIL
                     ENSRPGTRILMRSASPVIDFIPPFARERLRLDEATADRLHLLDRAGTYGRTLLADVTP
                     "
     gene            2270..2878
                     /locus_tag="PLP58_15325"
     CDS             2270..2878
                     /locus_tag="PLP58_15325"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HRJ09948.1"
                     /translation="MSNDASLAGYYRWHARIYDLTRWAFLFGRGRILRHAARHVVMPE
                     RILEVGCGTGKNLVELAELFPKAQILGLDLSKDMLDQARPKIHRHAPRVSLMQRAYDA
                     PVAIGRKFDLIVFSYSLSMINPGYEKVLDICREDLSARGMVAVVDFHESRQAWFRRWM
                     GVNHVRMEGQILESLRHRFTAAHCEVRHAYGDLWRYMFFIGR"
     gene            complement(2891..4243)
                     /locus_tag="PLP58_15330"
     CDS             complement(2891..4243)
                     /locus_tag="PLP58_15330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007416781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1501 domain-containing protein"
                     /protein_id="HRJ09949.1"
                     /translation="MPPPLTRRDFLARSGMGLAALGLADLQASKPHHAPKAKRVIHFF
                     LNGGPSHVDTFDPKPALARYAGKPVPSHRITERKTGAAFPSPFKFERRGQSGIEVSEL
                     FAKTAAHIDDIAVIRSMQAQVPNHEPSLMLMNCGDSIQPRPSLGAWLTYGLGSENSNL
                     PGFIAMCPGGMPIKGAENWQAAFLPGAFQGTYVDSKHEAVDRLIENIRSPHADTSVQL
                     RQLELLRRINAAHKAERSDPRLEARIQSFELAFRMQMEAADAFDVSQEPEHIRKMYGE
                     GVHARQTLIARRLLERGVRMVQLWHGAGQPWDNHDNIETNHRKLSNDIDQPIAALLSD
                     LKQRGMLEDTLVLWGGEFGRTPTVELAGGKSKLGRDHNHYGFSVWMAGGGIKGGTVHG
                     STDEFGFAAQENPTSIHDLHATILHLLGINHEELTYRYAGRDFRLTDVYGDVIRPILA
                     "
     gene            complement(4350..5117)
                     /locus_tag="PLP58_15335"
     CDS             complement(4350..5117)
                     /locus_tag="PLP58_15335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ09950.1"
                     /translation="MLELTTIKEAIHELCAADKQQLVSFIISQLRLENVKAEKRQISR
                     EAIQAWVAEDEADMKVFLDAGRTSQSAEDFWRLIQQRPEMFLGRPSISALWNFLNGWC
                     LACTKHGLADEALSLPADFHEWVAYRLRFHESTTGWCRMILERALDEAEGLKMFFQLK
                     DDHRTRVPTAIAHIEKFKLPDPHADRAEVVGSRMTLISYTTDPGFFVLHETQPTELAR
                     PTFYPCLSWFETFTGCRAIDLEIIDPARFDQVARHDV"
     gene            complement(5144..7759)
                     /locus_tag="PLP58_15340"
     CDS             complement(5144..7759)
                     /locus_tag="PLP58_15340"
                     /inference="COORDINATES: protein motif:HMM:NF019223.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1549 and DUF1553 domain-containing protein"
                     /protein_id="HRJ09951.1"
                     /translation="MTAALPIWIALTLAAGASGVAADHWAFQPLKPGKASSIDAYLPH
                     AHAPRADARTLIRRAHFDLTGLPPSDMSDSSDLSDEAYAKVIDSLLSSPHYGEQQARH
                     WLDIARYSDTKGYVYAREEKQWVHASAYRDWVVRAFNDDMPYDRFLLLQIAADQVVPP
                     NSPDLAAMGFLTLGRRFLGVTHDIIDDRIDVVMRGTQALTVACARCHDHKFDPIPTRD
                     YYALYGVFQSCAEALVACASGENAELAALEKKNRELMTKRREEQMKRNRARIADYLAA
                     QKHLDDYPEEVFSQILDEKDLNPFVVHRWKAWLEKNPGVELTEEMLRRPDSPAFVPDE
                     HISNIEMYFPTGITTELWRSQGDVDRFLIKTSTAPTYATILKDRALPSTPRVFTRGNP
                     LTKGDAVPRQFLSLFGNQTPFTKGSGRLELAQAIIDPRNPLTARVMVNRLWQHYFGKG
                     LVSTPSDFGKQGSPPTHPELLDWLATEFIRSGWSIKHMHRLIMLSEAYKQRSDGVMEN
                     WSDGQQSITPLLHHSNVPHRLSFEEARDAWLAAAGRLNLRVGGRPEPLFAASNTRRTL
                     YAFVDRENLPAVMRTFDFANPDLSIPQRTETTVPQQALFGLNHPFLVQQAKALVKRVS
                     TEKSPEGRIGLLYRYLFQRSPKADEMASALSFVAVDEKPVLVENDHAKAWQHGFGEWD
                     EKTGLVKSFTPLPHFTGHAWQGDEDWPNAKLGWAQLTPEGGHPGNNRQHAVVRRWIAP
                     ADGRYDLRSTLIHEPKVGDGIRAFVSHSRLGKLLGTKLHGSKADLSLTSIAFQKGDTL
                     DFIVDIDIGLNSDQFLWSPQLSPTQTIAGSGGDTPSEVWDAKKDFTAAPKTTLTSWEQ
                     LAQVLMLSNEFMFVD"
     gene            complement(7767..8993)
                     /locus_tag="PLP58_15345"
     CDS             complement(7767..8993)
                     /locus_tag="PLP58_15345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ09952.1"
                     /translation="MKPPPFLPLLCLSLATLPVSGATEGETEHLYWRIPGPDEPYVDT
                     QRGDRAFAFQGARIMLSEDNGRTWPHGAEFADAQNITFSVLLKNGNVLFATRERLFLS
                     TDKLKTHRELIVKRTDGSDYLPHKPVNPAAPGWYFHSLDGEHCFEVEGREMLVWGNYC
                     NVLGGPVPVNIYYSTDGGETVKLAYGFGQNPHFQQKGAPPGAPLLGEAGNPLIARHVH
                     GVTYNPAEGAFYACTGDIDRGHGKECHWLRGIYDAAADKWEWRVVISVDSNSRFKCGG
                     INFVNGRCWWIADANGPAIANRWDRGIFHCAPEDITDKARHTLAFNPGFESANMLYQD
                     GVFLATHCAPASTYVNGIIWSGDDGKTWAQHDLKEPGPRSGCRINRKNGEGWFRMDLR
                     KGWIERAEVLFLKSKT"
     gene            complement(9282..11864)
                     /locus_tag="PLP58_15350"
     CDS             complement(9282..11864)
                     /locus_tag="PLP58_15350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ09953.1"
                     /translation="MSARHPAMNHEIESFGHLMIARTTGQQPVRLSRSPDEVTFLAFD
                     ASIKRLVELHVLGKGQPVDAGTRQAVLRRAREVALFRGPSFTRILDVGEDQGLAYYTS
                     NLNDGEFVGEYVRRIGPPPPALVFALVHQLLEDLIRLKDQQQVVPSLHLDRVLVTTQE
                     DAFLQLRLYDLGLTSRETPQQGAARLVVEGCRLIFQLLTGREHQGENPDLFPAMTAVP
                     MNLRTTMRSALNDGGAHVPVSLERLKDDVREAHAALVSSIQARTSRKQLLVTPALQPK
                     SQIQSVLLENVPVETILGSRFLVVEDENFRRQPFSIPCVNAKNEQPVTVHLLPPSRIV
                     DKALYEAVPLQMWRFDPGRHPNILRSLSLWESPDWTFLTEEREPGFSLSRLLAERVCL
                     NPSEVLVLMRQVREGQEQALECGVERLDLHPSSIYLRVGKNGPTQARECERLMQKRVD
                     VWPPFHVKLRAHLTLRNLYEPPLVDKPELGEHPESHLADRDYRHRVFIGLASYLLTGE
                     RQTGGRPVFPETMPEVLGGYICECLEKCRQHGAAPSPAEFLARFEELMNGPAAPDLLT
                     RMRGAMVNVEDMESAGSVSDFDDDDYADSDSDTPHGTAPARLKPVAFTRKSSRRRGKP
                     PYLAWAAGIAITAVLAWLLLPSAQESSPTSASMEGADPDQALQHAAHSHPEESPPPPS
                     KSEPVAARQSAEASDPPAEPPAPSAETPPPAPKPVLTAAQEGPQKTEAKLARIESAED
                     SAPSAPVVASQAGAQPSVEKTEASPPQAPATAAAPHPTAADGNNPPVGETSGKAGPRV
                     AKPVPNPVIIRKAILPTPDEIAKYKQSLNQTPPPVPAKSDTPPEEDVERIGTTPTPPN
                     LARP"
BASE COUNT         2269 a         3591 c         3971 g         2381 t
ORIGIN      
        1 ggctgggcga catcgcaata aaggcgggtg agattggtca tgcgccaaac aacgcgcccc
       61 tccgccgccc gcgcgccgcc gatcttgcgc agtggtgagc cggacttggg cggagctttt
      121 cgaaaaaaag gcttcctttt cttcccgccc cgatttttat ccgttccctc ccatgaagct
      181 cccgaccctt tccctcctcg ctctctcatt cctcctggca tcctgcgcgc gcgagcgtgt
      241 catcccggtc catgtggcca ccacgccggt ggccgagccg aacgaggcga agatgaagcg
      301 ccacgagagc ttcaacgagg tctccaaaca gggcgaggcg acgctcgttt ttctcggcga
      361 cagcatcacc cagggctggg agggcaaggg caaggccgcc tgggagaaat attatgcttc
      421 gcgcaaagcc gccaacttcg gcatcggcgg cgaccgcacc gagcatgtgc tctggcgcct
      481 tgagaacggc aacttcgacg gactcaaacc caagctcatc gtgctcatga tcggcaccaa
      541 caacaccggc catcagaatc gtccgatgcc ggagatcaac aatcaaatct acacatgcag
      601 cgccgaacag accgccgacg gagtcgaact catcctgcgc aagctgcgga aaaaggcccc
      661 ctttgccgac atcctcctgc tcggcatctt cccgcgcgga gagactcccg aggacccgat
      721 gcgcaaacaa aatgaggcca ccaacgtgct gatctccaaa ctcgccgacg gcaaaaccat
      781 tcatttcatg gacatcggca agaccttcct ccagtcggat ggaactctga ccaaggaaat
      841 catgcccgac ctgctgcatc tctctgaaaa aggctacgag atgtgggccg gggccatcga
      901 gccgaagatc aaggaactgc tcggcgagtg agcgcgcagc cacgctggag agcggccatc
      961 cggcggtgcg ggaagacatg acacggcggt ggcgcgagct gctgaaaccc catgtgtcaa
     1021 ccgggccgtg agatggcgat tttttcacac gaagccgcct gcctgactgc gcggacgggc
     1081 ttttgtggcg ggtcatgccc gtcacgctca agtcccgcac gccgctccgc catctcgccc
     1141 aggatgtgat cttcaaccgc gtgcatggcg gcaacctcgt ctataacacc tgctgggagg
     1201 acccgcggcc cgatctggaa atgctggacc tgcgcgcgga cagccggctg gtgatgatca
     1261 cgagcgccgg ctgcaacgcg ctggactatc tgctggctgc gccggcgagg atacatgccg
     1321 tggacatgaa cccgcgccag aacgcgctgc tgcagctcaa actggcgatg atccagcgcg
     1381 gggacttcga agatttcttc gccctctttg gccgcggggc gcatccgcgc ttccgcgaga
     1441 ttttggcgga cctggacctg ccgccctacg cggcgcgcta ctggcgcgca aagcactact
     1501 attttcaaag cacgccgctg aacccctcct tttactaccg gggcacggca ggccagatgg
     1561 cgtggatcac gctgcaaagc ttcacgcgcg gcagcaggaa agtgcgcggc tacatccacg
     1621 ctttgcttga gacaggcacg ctggaggagc agcgccggct ttatgatgag gtgaaaccgg
     1681 atctctggag cggactcgtc tcctggcttc tgcgccagcc cgtgtccatg accatgctcg
     1741 gagtcccgcg cgcgcaggtg cggctcatcg aagcaaactt ccccggcggc atcctgggct
     1801 acatccggca aaagctggag catgtgctga cggagacgct ttttctcgag aactacttct
     1861 ggcgtgtcta tctcacgggc cgttacacgc gcgaatgccg cccccgctac ctgcgcgagg
     1921 agcatcaacc gctgctgcga gagcgcgcgg agcggatcgc cacgcacagc acgacggtgg
     1981 caaacttttt gcgcgagcat ccgggagcct actcgcattt cgtgctgctc gaccatcagg
     2041 actggctggc cgcgcacagg ccgcaggcgc tggaggagga gtggcgtctc atcctggaaa
     2101 acagccgccc tggaacgcgc atcctcatgc gctcggcgag cccggtcatt gacttcatcc
     2161 cgcccttcgc ccgcgagcgt ctgcgcctgg acgaggcaac cgccgaccgc ctgcacctgc
     2221 tcgaccgcgc gggcacctat ggacgcaccc tgctggcgga tgtgacgcca tgagcaacga
     2281 cgcctcactc gccggctact accgctggca cgcgcgcatc tacgacctca cccggtgggc
     2341 ctttttgttc ggccggggcc gcatcctgcg gcacgcggcg cgccatgtgg tgatgccgga
     2401 gcgcatcctc gaggtgggct gcggcacagg aaaaaatctc gtcgaactgg cggagctctt
     2461 cccaaaggcg caaatcctcg gcctggatct ctcaaaagac atgctggacc aggcacgccc
     2521 gaaaatccac cgccacgcgc cccgcgtgtc cttgatgcag cgggcctatg acgcgccggt
     2581 ggccatcggg cgaaagttcg acctcatcgt cttcagctac tcgctttcga tgatcaaccc
     2641 cggctatgag aaggtgctgg acatctgccg cgaagacctg agcgcgcgtg gcatggtggc
     2701 cgtggtggat ttccacgagt cgcgccaggc gtggttccgc cgctggatgg gcgtgaacca
     2761 tgtgcgcatg gaggggcaga tcctggagtc gctgcgccac cgctttaccg ccgctcattg
     2821 cgaagtccgc catgcctacg gcgatttgtg gcgttacatg ttcttcatcg ggcggtgaaa
     2881 atatcccgcg tcaggccagg atcggccgga tcacatcccc atagacatcc gtcaggcgga
     2941 agtcacgccc ggcgtagcgg taggtcagct cctcgtggtt gatgccgagg aggtggagga
     3001 tcgtggcgtg cagatcgtgg atgctggtcg ggttttcctg cgcggcgaag ccgaactcgt
     3061 cggtgctgcc gtgcaccgtg ccgcctttga tgccgccgcc ggccatccac acgctgaagc
     3121 cgtagtgatt gtgatcgcga ccgagcttcg atttgcctcc ggcgagttcc acggtcgggg
     3181 tgcggccgaa ttcaccaccc catagcacga gcgtgtcctc cagcatgccg cgctgcttca
     3241 gatcgctcag cagtgccgcg atgggctggt cgatgtcgtt cgagagcttg cggtgattcg
     3301 tctcgatgtt gtcgtgattg tcccagggct gcccggcgcc atgccagagc tgcaccatgc
     3361 gcacaccgcg ctccagcaga cgccgcgcga tgagcgtctg ccgcgcgtga acgccttcgc
     3421 catacatttt gcggatgtgc tcgggctcct gcgacacatc aaacgcgtcc gccgcctcca
     3481 tctgcatgcg gaaagccagc tcgaagctct gaatgcgcgc ctccaaccgc ggatcgctgc
     3541 gctcagcctt gtgcgcggca ttgatgcgac gcaacagctc gagctggcgg agttgcacgc
     3601 tcgtgtcggc gtgcgggctg cggatgtttt cgatcaaacg gtccaccgct tcgtgctttg
     3661 aatcgacata ggtgccctga aacgcaccgg gcaaaaacgc cgcctgccag ttctccgcgc
     3721 ctttgatcgg catgccgccg ggacacatcg cgatgaagcc cggcaggttc gagttctcgc
     3781 tgccgaggcc atacgtcagc catgcgccga ggctcggacg cggctgaatg gagtcgccgc
     3841 aattcatgag catcagcgaa ggctcgtggt tcggcacctg cgcctgcatc gagcggatca
     3901 ccgcgatgtc atcgatgtgc gccgccgttt tcgcgaacaa ctcgctcacc tcgatgccgc
     3961 tctgtccgcg ccgctcgaat ttgaacggcg aaggaaaggc cgcgcccgtc ttccgctccg
     4021 tgatgcgatg actcggcacc ggtttgcccg cataccgcgc cagcgccggc ttcggatcaa
     4081 acgtatccac atgcgacggc ccgccattga ggaagaaatg aatcacgcgc ttcgccttcg
     4141 gcgcgtggtg cggtttggaa gcctgcaaat ccgccaaacc cagcgcagcc agacccatgc
     4201 ccgagcgggc gaggaagtct cggcgggtga gtgggggagg catgttgatc aaacgggact
     4261 tcaagggctt tgcttgccaa cggcagcttt gttcagacct tgatctggga ttgcatgaca
     4321 gtatggcaaa cgagagaaaa ccacagagcc tacacgtcat gcctggccac ttgatcgaat
     4381 cgagcaggat cgatgatttc tagatctatg gctcggcaac ctgtgaaagt ctcaaaccaa
     4441 gatagacacg gataaaaggt tgggcgcgct aattctgtgg gctgggtttc gtgcagaaca
     4501 aaaaaaccag ggtcggtcgt atagctgatc aaagtcattc tggatccaac cacttcggcc
     4561 cggtcagcat gcggatctgg aagtttgaac ttttcaatat gcgcaattgc agtgggcacg
     4621 cgggtgcggt gatcgtcctt gagctggaaa aacatcttca atccctctgc ctcgtccaga
     4681 gcacgctcta aaatcatcct gcaccatcct gtagttgact catgaaacct aagacgatac
     4741 gcaacccatt cgtggaaatc agccggtaga gacagcgcct cgtcagccaa tccatgcttt
     4801 gtgcacgcga gacaccaacc attcagaaag ttccacagcg ctgagattga aggccggcca
     4861 aggaacatct ctgggcgctg ctgaatcaac cgccaaaagt cctccgcaga ctgggacgtt
     4921 cttcctgcat ccaaaaacac tttcatgtct gcctcgtctt ccgcaaccca ggcttggatt
     4981 gcttctctcg aaatctgacg tttctcagct ttgacgttct ccagcctaag ctggctgatg
     5041 ataaagctga ccagttgctg cttgtccgct gcgcacaact cgtggatggc ctctttgatg
     5101 gtcgtcaatt caagcattcc acacgcgtgc tgagaaaacg atctcaatca acaaacatga
     5161 actcgttcga gagcatgagc acctgcgcga gttgctccca agaggtgagc gttgttttcg
     5221 gcgcggcggt gaagtctttt ttggcgtccc agacttcgga tggcgtgtca ccgccggaac
     5281 cggcgatggt ttgagtgggg ctgagttgcg gggaccagag gaattggtcg ctgttcaggc
     5341 cgatgtcgat atcgacgatg aaatcgaggg tgtcgccttt ctggaaggcg atggaggtga
     5401 ggctgaggtc ggctttggag ccgtggagct tggtgccgag caatttgccg aggcgggagt
     5461 ggctgacaaa ggcgcggatg ccgtcgccaa ccttcggttc gtggatcaag gtggagcgga
     5521 gatcgtagcg gccatcggca ggcgcgatcc agcgacgcac gacggcgtgc tgacggttat
     5581 tgccgggatg gccgccttcg ggggtgagtt gtgcccagcc gagtttggca ttcggccagt
     5641 cttcgtcgcc ttgccaggcg tggccggtga agtgtggcaa gggcgtgaag ctcttcacga
     5701 ggcctgtttt ttcatcccac tcgccaaaac catgctgcca ggccttcgcg tggtcgtttt
     5761 cgacgagaac gggcttttca tcgacggcaa cgaaggacag cgcggaggcc atttcatcgg
     5821 ctttggggct gcgttggaac aaatagcgat acagcaggcc aattcggcct tcgggcgact
     5881 tttcggtcga aacgcgtttc acgagcgctt tggcctgctg aacgagaaag gggtgattga
     5941 ggccgaaaag ggcctgctgc ggcacggtgg tctcggtgcg ttgcggaatc gagagatcgg
     6001 gattcgcgaa atcgaaggtg cgcatcacgg cgggcaagtt ttcgcgatcg acgaaggcgt
     6061 agagcgtgcg gcgcgtgttg ctggcggcaa aaagaggctc gggacggcca ccgacgcgca
     6121 gattgagccg acctgcggcg gcgagccagg catcgcgggc ttcttcgaag ctcaagcggt
     6181 gcgggacgtt ggagtgatgg agtaatggag taatggattg ctggccatca ctccaattct
     6241 ccatcactcc atcactcctc tgcttgtagg cttccgacag catgatgagc cggtgcatgt
     6301 gcttgatgct ccagccgctg cggatgaact cggtggcgag ccagtcgagg agttcgggat
     6361 gcgtgggcgg gctgccttgc ttgccgaagt cgctcggggt gctgacgagg cctttgccga
     6421 agtagtgctg ccagaggcga ttcaccatga cacgggcggt gagcggattg cgcgggtcga
     6481 tgatggcctg cgcgagctcg agccggccgc tgcctttggt gaagggcgtt tgattcccga
     6541 agaggctcag gaactgacgc gggacggcat cgcctttggt gaggggattg ccgcgggtga
     6601 aaacgcgcgg cgtgctcggc agcgcgcgat ctttgagaat ggtggcgtaa gtcggcgcgg
     6661 tgctggtttt gatgagaaag cggtccacat cgccctgcga tctccacaac tcggtggtga
     6721 tgccggtcgg gaaatacatt tcgatgttcg agatgtgctc atcaggcacg aaagcgggtg
     6781 aatcgggccg tctgagcatc tcctcggtca attcgacgcc gggattcttt tcgagccagg
     6841 ctttccaacg atgcacgacg aaagggttga ggtctttttc gtcgaggatc tggctgaaga
     6901 cctcttccgg gtaatcatcg agatgtttct gcgcggcgag gtagtcggcg atgcgcgcgc
     6961 gattgcgctt catctgctcc tcgcggcgtt tggtcatgag ttcgcgattc tttttctcca
     7021 gcgcggcgag ttcggcgttt tcgccgctcg cgcaggccac gagggcctcg gcgcagcttt
     7081 ggaagacacc gtagagcgcg tagtagtcgc gcgtgggaat gggatcgaat ttgtgatcgt
     7141 ggcagcgtgc gcaggcgacg gtgagcgcct gcgtgccgcg catgacgacg tcaatgcggt
     7201 cgtcgatgat gtcatgcgtg acgccgagga agcgccgccc gagcgtgaga aagcccatcg
     7261 cggcgagatc tggtgaattt ggcggcacga cctgatccgc ggcgatctgc agcagcaaaa
     7321 agcggtcgta gggcatgtca tcattgaagg cgcggacgac ccaatcgcgg taagcggagg
     7381 cgtgaaccca ttgtttttcc tcacgggcat agacgtagcc tttcgtgtcg gaataacggg
     7441 cgatgtccag ccagtggcgg gcttgttgtt cgccgtagtg ggggcttgag aggagagagt
     7501 cgatgacctt agcgtaagct tcgtcggaca ggtcggacga gtctgacatg tccgacggtg
     7561 ggaggccggt gagatcaaag tgagcgcggc ggatgagggt gcgggcgtca gcgcgtgggg
     7621 cgtgtgcgtg tggcaggtag gcgtcgatgc tgctggcctt gccgggtttt agcggctgga
     7681 aggcccagtg gtcggcagcc accccgctcg cgccggcggc gagagtgagg gcaatccaga
     7741 ttggaagggc cgctgtcatg cggatatcag gttttcgatt tgagaaagag aacctcggcg
     7801 cgctcgatcc agcctttgcg gaggtccatg cggaaccagc cctcgccgtt tttcctgttg
     7861 atgcggcagc cggagcgcgg gccgggctct ttgagatcat gctgcgccca cgtcttgccg
     7921 tcgtcaccgg accagatgat tccgttcaca taagtggagg ccggggcgca gtgggtggcg
     7981 aggaagacac catcctggta aagcatgttc gcggactcga agccgggatt aaaagcgaga
     8041 gtgtgcctgg ccttgtccgt gatgtcttcc ggagcgcagt gaaagatgcc gcgatcccaa
     8101 cgattcgcga tggcggggcc gttggcatcg gcgatccacc agcagcggcc gttgacaaaa
     8161 ttgatgccgc cgcatttgaa gcgcgaattc gagtccacgc tgatgacgac ccgccattcc
     8221 cacttgtccg ccgccgcgtc ataaatgccg cgcagccagt ggcactcctt gccgtggccg
     8281 cggtcaatgt cccctgtgca ggcgtaaaaa gcaccctcag cgggattgta agtgacgccg
     8341 tgcacatggc gggcgatgag cgggttcccc gcctcgccca gcagaggcgc gcctggcggc
     8401 gcgccctttt gctggaagtg cgggttttga ccgaagccat aggccagctt caccgtctcg
     8461 ccgccatcag tggagtaata aatgttcacc ggcacgggac cgccgagcac gttgcagtag
     8521 ttcccccaca cgagcatctc acgcccttcg acttcgaagc aatgctcgcc gtcgagtgaa
     8581 tggaaatacc agcccggcgc ggcggggttc accggcttgt gcggcaggta gtcgctgccg
     8641 tcggtccgct tgacgataag ctcgcggtgc gtcttgagct tgtcagtgct taggaacagc
     8701 cgctcccgcg tggcgaagag gacgttcccg ttttttaaca gcacgctgaa agtgatgttc
     8761 tgcgcgtcgg caaactccgc accgtgcggc caggtgcgcc cgttgtcctc ggaaagcatg
     8821 atgcgcgctc cctggaaggc gaaagcacgg tctccccgtt gtgtgtccac atagggctcg
     8881 tccggtccgg ggattcgcca gtaaaggtgc tctgtctcac cctcagtggc gcctgacaca
     8941 ggcagggtgg caagggaaag gcacaggagc ggcaggaaag gcgggggctt catggcaggg
     9001 cctctcaata cgcaccgctg gagccggacc ctgcatcgcg ggcgtatttg gtcaggccgg
     9061 gtatgtcagg ctgcgcggct tgtgcagtgc gcattttggc gacggtagcg ctggcgctac
     9121 catgaggtga ataccctggc ccgtcgaaca acgctattca tccggcatca agacagcacg
     9181 ctgaatcgcc catccacttc tctttgcgga tggtgtgctt tcttggtgcc gcattaataa
     9241 agggaagcgg cggcccgtgc cggtccggca cccagaccac atcaaggccg cgcgaggttt
     9301 ggcggagtcg gcgtggtgcc aatgcgctcc acgtcttcct caggcggggt gtcagacttg
     9361 gctggcacag gaggcggagt ctggttcagg ctctgcttgt atttggcgat ctcatccggc
     9421 gtgggcagga tggccttgcg gatgatgacg ggattcggca caggcttggc gacgcgcggg
     9481 ccggccttgc cagaagtttc gccaactggc ggattgtttc catccgcagc ggttggatgt
     9541 ggcgcggcgg cggtggccgg ggcctgcggc ggggatgctt ctgttttttc cacgctgggc
     9601 tgcgcgcccg cctgggaggc aacgacgggc gcggaaggcg cggagtcttc cgcagactcg
     9661 attctggcaa gtttggcctc ggttttttgc ggtccctcct gcgcggcggt taacaccggc
     9721 ttgggcgcgg gcggaggtgt ttcagcggaa ggtgccggcg gctcggcagg cgggtctgag
     9781 gcttcggcgg actgccgggc ggcgacgggc tccgacttgg atggaggcgg cggcgactcc
     9841 tctggatgcg agtgggcggc gtgctggaga gcctggtcag gatcggcgcc ttccatgctg
     9901 gccgaggttg gcgaggattc ctgggcggag ggcagcagca gccaggcgag cacagcggtg
     9961 atggcaatgc cggccgccca ggccaggtag ggtggcttgc cgcggcggcg gctgcttttg
    10021 cgggtgaagg cgacgggttt gagccgtgcg ggcgcggtgc cgtgaggagt gtcggaatcg
    10081 gaatccgcgt agtcgtcgtc atcgaagtcg ctgacactgc cggcgctttc catgtcctcg
    10141 acattgacca tggcacccct catgcgggtg aggaggtcgg gcgcggcggg gccgttcatc
    10201 agttcttcaa aacgcgcgag aaactcggcg ggggaagggg ccgcgccatg ctgacggcat
    10261 ttttccaggc actcgcaaat gtacccgccc aggacttcgg gcatggtctc ggggaatacg
    10321 ggcctgccgc cagtctggcg ctcgccggtg agcaggtaac tggccagtcc gatgaagacg
    10381 cggtggcggt agtcccggtc ggcgaggtgt gattcggggt gttcgcccag ctccggcttg
    10441 tccacgagcg gcggctcgta gagattgcgc agggtgaggt gtgcgcgcag cttgacatga
    10501 aagggcggcc acacgtccac gcgcttttgc atcaggcgct cgcactcgcg ggcctgggtg
    10561 gggccgtttt tgccgacgcg cagataaatg ctggaggggt ggagatccag cctttcgacg
    10621 ccacattcca gggcctgctc ctggccttcc ctcacttggc gcatgagcac cagcacctcg
    10681 gaggggttga ggcagactcg ctcggcgagc agtcggctga gggagaaacc gggttcgcgc
    10741 tcctcggtga ggaaggtcca gtcagggctc tcccacagac tgaggctgcg caaaatgttc
    10801 gggtgcctgc cgggatcgaa gcgccacatt tgcagcggca cggcctcgta cagcgccttg
    10861 tccacgatgc gcgacggcgg cagcagatgg acggtgacag gctgctcgtt cttggcgttg
    10921 acgcagggga tggagaaggg ctggcggcgg aaattttcgt cctccaccac gaggaaacga
    10981 ctgccgagga tggtctcgac agggacgttt tcgagcagga cgctctgaat ttgggacttc
    11041 ggctgaagcg cgggagtgac gaggagctgc ttgcgcgagg tgcgcgcctg gatgctgctg
    11101 accagggcgg cgtgagcctc gcgcacgtcg tccttgagcc gctccaggga cacgggaaca
    11161 tgcgcgccgc cgtcgttgag ggcgctgcgc atggtggtgc gcaggttcat gggcacggcg
    11221 gtcatggcgg ggaaaaggtc gggattttca ccctgatgct cgcggccggt gagcagctgg
    11281 aagatcaaac ggcagccctc gaccacgagc cgtgcggccc cctgctgggg agtctcgcgc
    11341 gaggtgaggc ccaggtcgta aagccggagc tgcaaaaagg cgtcctcctg cgtggtcaca
    11401 agcacgcgat ccagatgcag cgagggcacc acctgctgct ggtccttgag ccggattaaa
    11461 tcctccagca actgatgcac gagcgcgaac acgagcgcgg ggggcggcgg cccgatgcgc
    11521 cgcacatatt caccgacaaa ttcgccatcg ttcagattgc ttgtgtaata ggccagcccc
    11581 tgatcctccc ccacatcaag gatgcgggtg aaggaaggcc cgcggaacaa tgccacctcc
    11641 cgcgcgcggc gcagcacagc ctggcgggtg cccgcgtcca ctggctggcc tttgcccagg
    11701 acatgcaatt cgaccagcct cttgatcgag gcgtcgaaag ccagaaatgt cacctcatcc
    11761 ggcgagcgcg agagccgcac cggctgctgg ccggtggtgc gagcgatcat caagtgaccg
    11821 aagctctcga tttcgtgatt cattgcaggg tgcctggcgg acatgtgatg aatgccaagc
    11881 atgaggctgg agtgcctggg aggaaaagaa agatttcagc agtgctttat tgaaattata
    11941 cgacaaacta tcaaatgata catattttac agaaaatata agaaaatcaa aggtattttt
    12001 tagcctcggt gcttcaacca cgaggcttcg cctgctccga gtcgcaattt tagcgcaacc
    12061 cttgtgccat gtccgcgttt caaggtcggc ctctgaacaa atcatgaaaa cgctgtcttt
    12121 cgtctgtata cccctgctcc ttctcgtctc gctcaccgac gctgagacga cagccccgga
    12181 tggcaactcc aacttccgca cttggcgcga cc
//
LOCUS       DAOMYJ010000160        11933 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Prosthecobacter sp. isolate AMR_MDS_5629
            NODE_1778_length_11933_cov_22.357299, whole genome shotgun
            sequence.
ACCESSION   DAOMYJ010000160 DAOMYJ010000000
VERSION     DAOMYJ010000160.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867850
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prosthecobacter sp. (wastewater metagenome)
  ORGANISM  Prosthecobacter sp.
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Verrucomicrobiaceae; Prosthecobacter.
REFERENCE   1  (bases 1 to 11933)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11933)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.30
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:34:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,742
            CDSs (total)                      :: 4,684
            Genes (coding)                    :: 4,676
            CDSs (with protein)               :: 4,676
            Genes (RNA)                       :: 58
            tRNAs                             :: 55
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11933
                     /organism="Prosthecobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1778_length_11933_cov_22.357299"
                     /isolate="AMR_MDS_5629"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1965333"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1110
                     /locus_tag="PLP58_15355"
     CDS             <1..1110
                     /locus_tag="PLP58_15355"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070007 - glutamic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PrsW family glutamic-type intramembrane
                     protease"
                     /protein_id="HRJ09954.1"
                     /translation="HAALLMRDQDEAGAMRALMREGRLFADAFEAREDALRLALRLKD
                     ADALRAMKAVPDWLETSPPALQHQAGTLLRDPWLQWRGLLLHRLEHVPYGALGMAFFA
                     CLIWYAAFVLHGGSGSRRWLWPVPPVVAGVLSVWPVLTVASWQETANGMSADAPFPHD
                     LWYYVAGVGLREELAKLAAAALFMPWLLWSRVPGRALLTGAFAGLGFALEENINYYTE
                     FGGGVALVRLLTANFLHAALTGILTHALYTALRSRFTRVDGFLAALPAVVAVHGLYDY
                     TASSSIGGMDFAAMVILAFVAWHFLDVMEQEAAPARRWVSPGSVVVIGAALLIAIIFI
                     STALENTGRAGLAAAAIECVAVLPLMFIYWRRLGG"
     gene            complement(1175..1378)
                     /locus_tag="PLP58_15360"
     CDS             complement(1175..1378)
                     /locus_tag="PLP58_15360"
                     /inference="COORDINATES: protein motif:HMM:NF023056.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3253 domain-containing protein"
                     /protein_id="HRJ09955.1"
                     /translation="MRDEPAKIRLALLALAAERKRSFCPSEVARRLAEDWRNLMPMVR
                     AAAADLVDEAGWPAHSAGSPLIR"
     gene            complement(1521..2342)
                     /locus_tag="PLP58_15365"
     CDS             complement(1521..2342)
                     /locus_tag="PLP58_15365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009965194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HRJ09956.1"
                     /translation="MKQKTWLITGCSRGLGRAIAEAALEAGQRVIATARDASVIADLE
                     KPSMCEVMTLDVTDQAQARDVVAASKPDVIVNNAGQGLLGAVEECGEEQIRRCMETNF
                     HGPLNVIRAALPGLRSRRSGHIINISAAAAISNYAGFGIYGAAKGALEMLSESLRLEL
                     APLGIHVTLVQPGPFRTAFISRSLEKAEHEIGDYAASSGKFARLIEGMDGRQPGDPAR
                     AARAIMAAASSDKPPMRLVLGRYANEKARRRGAEVEKERAEWEAVGSPCEFTSPA"
     gene            complement(2339..2818)
                     /locus_tag="PLP58_15370"
     CDS             complement(2339..2818)
                     /locus_tag="PLP58_15370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009965193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan-rich sensory protein"
                     /protein_id="HRJ09957.1"
                     /translation="MKPPHSRLRGGLVLAAFLFATFAAPALSAFFPGPGDWYAELVKP
                     AWNPPGWVFGPVWTLLYTLMAVAAWLVWRRAGWTKPHGFYLAQLVLNAAWTPVFFGAR
                     EIGWALVVIVALWAAILSTLLSFHKVSRVSGWLLAPYLAWVSFASVLNFTLWRLNPA"
     gene            complement(2815..4353)
                     /locus_tag="PLP58_15375"
     CDS             complement(2815..4353)
                     /locus_tag="PLP58_15375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009965192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cryptochrome/photolyase family protein"
                     /protein_id="HRJ09958.1"
                     /translation="MPRVSRLILVLGDQLDLESEAFDGFNAGEDAVWMAEAAAESVKV
                     WSTKARIAMFLSAMRHFREALSGRGWRVFYHEMGAHESLGSALRAEIARSRPEKLAMV
                     RAGEWGVQWEIEAAAADAGTPLEVREDGHFLCTTAEFARHARGRKQLRMEFFYREMRQ
                     KTGVLMQEGKPVGGRWNYDHDNRASFGRCGPGLRVPPRKFEPDSITRGVIALVNDTFH
                     AHPGTLDDFDWPVTAAEAGLALEDFIARRLADFGRYQDAMWEGEPWLFHSRLSAALNL
                     KLLDPREVIEAAERAWRDGAAPLESVEGFIRQILGWREYVRGIYWLHMPGYLERNAMN
                     AELPLPGFYWTGETEMACLRAVIKQTLRHGYAHHIQRLMVTGLFALLYGAHPRRVHEW
                     YLAVYVDAVEWVELPNTLGMSQYADGGVMASKPYIASGKYIQRMSNHCAGCRFDPAKS
                     TGPSACPFTTLYWDYLLRHEPVLGKNPRMSMQVRNLARLDGKQKAAIQKQAGEIRAAY
                     SSPS"
     gene            complement(4396..5139)
                     /locus_tag="PLP58_15380"
     CDS             complement(4396..5139)
                     /locus_tag="PLP58_15380"
                     /inference="COORDINATES: protein motif:HMM:NF020037.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HRJ09959.1"
                     /translation="MNDPFAAHEVAEALFDELPDVVFFIKDTSGRYARVNQTLADRCA
                     GGDKKRLLGKRPAEVFPAALAAGYSRQDEQVLRTGRTVRDQLEMHIYPRSVAGWCLTT
                     KHALRDARGHVIGVAGISRDLNAPGDKAPGFAELSAALRRMRRDFATSLRIEEIAREA
                     GMSMHQFEQKMRRLFQMSPLQLLHKMRLDEAIRLLRETDKSLGEIALETGWCDQSAFT
                     RHFSRHTGMSPGRFRAFEVARATGKDAAG"
     gene            5222..6124
                     /locus_tag="PLP58_15385"
     CDS             5222..6124
                     /locus_tag="PLP58_15385"
                     /EC_number="4.1.3.3"
                     /EC_number="4.2.1.41"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009964456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009436 - glyoxylate catabolic process
                     [Evidence IEA];
                     GO_process: GO:0071704 - organic substance metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HRJ09960.1"
                     /translation="MSQSSIEWSGVFPAVVTHMHPDQSLDLPACARHWEALIESGVSA
                     LIVCGSLGENQTLPPEEKRTVVKHALAVAAGRVPVVCGVAEMSTQAAIRYLHDCEKLG
                     AAGFMVMPPMVYKPDPRETQHWFRTLAAATPLTWMLYNNPIGYHTDVTPEMFVELADI
                     PNLLAIKESSANTRRITELRNLTGDRYRIFTGVDDLFLESAILGIDGWVCGSGIAFPK
                     ENQQLWDLAQAGRWDECRALYRWSQPLMKLDTHLHFVQYIKLLCQETGLGNEWVREPR
                     LPLAGAEREQVLNIIRGALAKRPA"
     gene            6224..7972
                     /locus_tag="PLP58_15390"
     CDS             6224..7972
                     /locus_tag="PLP58_15390"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HRJ09961.1"
                     /translation="MLFIAVDDMRCDLGCYGVKHVFTPNLDRLAASGVLFSRAYCQQA
                     VCNPSRVSLMTGMRPDSTKVWHLSTEMRSVMPDAVTIPQHFRKHGYYALGFGKIFHNP
                     WPDNVSWDEPHVWPEKPSWSKAATGKLAEFREQMRAEGKPEARIDRMRAAATEVLDIP
                     DEEHFDGAIGEQAVAAMRRLAAEKQPFFLAAGFVRPHLPFVVPRKYWDLYDRAKIPLA
                     TNDHLPHGAPSMAFGEFQGGFYELRDYMDYADARWPREGPLSEAQQRELKHGYYAAVS
                     YIDAQVGRLLDELDRLGLAGNTIVVLWSDHGWKLGEHGGWCKQTNYEIDTRAPLMIRM
                     PGAKANGTKSSALVEFVDVYPTLADLAGLPVPEKLEGTSLKPLLNGTATKVKDAAFSQ
                     FPRREKDGHHMGYAMRTDRYRYIEWLELSSRKITAQELYDHESDPDENQNIATKAEHA
                     DLLASLSAQMWKTLPRPTFQWQEPASMASAAATPTSLPWHPATGAALPKSEPKGPKLA
                     VTFQNQSTAAVELIWISPQGTEKHYKTLQPKDAFNIRSSGGAVWVIKDASNKPLGHFQ
                     IHGTADKPVKAIIPAP"
     gene            7969..9657
                     /locus_tag="PLP58_15395"
     CDS             7969..9657
                     /locus_tag="PLP58_15395"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HRJ09962.1"
                     /translation="MKTLCFILGIFVAASSVCVAASEKPNVLFISVDDLNDWIGCLGG
                     HPQTKTPNFDRLAKSGTLFTNAHCAAPQCNPSRTAILTGIPPYRSGVYHNGQKMRDVL
                     PDAEIMPKFFSRHGYWSAGSGKLLHYFIDAASWDDYFPAKEKENPFPPTFDPPNRPIS
                     LPIGGPWQYVETDWAALEVSDEVCGGDWSVTKWIGEQLQRPHDKPFFLACGIYRPHEP
                     WFVPKEYFEPFPLESIQPGPGYKEDDLDDLPPSGQKMGPNRYFEHIQKQGQWKQAIQA
                     YLASIHYADAMLGRVLDALESGPHRDKTIVVLWSDHGWHLGEKQHWQKYTGWRICTRV
                     PLMIRVPGGKPGAMCDQPVSLTDLFHTVTDLCGLPAPEGISKNSLVPLLQNPDAVWPY
                     AAVTQLGVPDEYAISTKAWRYIHYRNGDEELYDIVNDPHEWNNLAAKPEHAAKLTELR
                     AFAPKNPVPVPQVPLASLPELKWHPSRDSAPPSKPDGGTFDVVFINQHTQPVELCWMS
                     PTGEPKSYGMIQPKGQKRKSTRPGAVWFIRDGSQQPLGHFQIGDRTSKAIIPAQ"
     gene            9654..10517
                     /locus_tag="PLP58_15400"
     CDS             9654..10517
                     /locus_tag="PLP58_15400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009964459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline racemase family protein"
                     /protein_id="HRJ09963.1"
                     /translation="MIRIPIIDSHTGGEPTRVVLGGVTADNLETYRRAIICEPRGHEV
                     IVGALLVEPKDASCAAGVIFFNNVGLLGMCGHGMIGLIATLKHLGRISAGEHRVETPV
                     GIITATLHDDGRVSIRNVPSYRQAKGVKAGEVTGDVAWGGNWFFLVSDHGLDLTLKNV
                     PQLTQASLAMREAVRAAGYPEVDHVELFGPPHDPANHSRNFVLCPGGEYDRSPCGTGT
                     SAKLACLAADGKLAPGETWRQESILGSVFEGSYQNGEGGIIPTITGEAFITAESTLLL
                     DPADPFQLGIA"
     gene            10514..>11933
                     /locus_tag="PLP58_15405"
     CDS             10514..>11933
                     /locus_tag="PLP58_15405"
                     /inference="COORDINATES: protein motif:HMM:NF013123.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DPP IV N-terminal domain-containing protein"
                     /protein_id="HRJ09964.1"
                     /translation="MKSLLFFTLSLSAFAADFEAALSFPKRTENKVFRDRVQANWLPD
                     GKTFWYRVQTAPGKSEFVLIDANNGSRKTAATLKQLGLPEKEAAKTSTMKIELRRTRR
                     TGESSGLKFINQLEEDVELYWINQEGGHVRYGGVRAGTEREQHTFDGHVWLITSRTGE
                     HLAVVEGAAQAQTFIIDGKGISKANTEASKKPERGFRAPDGSCVLFTEADKVERRKVT
                     IVESTPNGSVQPKLKVFDYIKPGDPLPKPQWVIEKSDGTKLRVARDLYENPFTESGEL
                     DAVWAPDSSEFYFNYNQRGHQLYRILAVNAKTGAVRVVVEETSKTFIHYSGKSWRHWL
                     HSTGELLWMSERDGWCHLWLYDAKKSSPKHQITKGAWPVREVLQVDEAKKEIWFLASG
                     LRAKEDPYHTHLCRVNFDGSGFQQLTEGDGNHRVEFSPNRDFFIDTWSRADKPPVMEL
                     RRSRDGGLVCDLEKAEVSALFAA"
BASE COUNT         2289 a         3996 c         3497 g         2151 t
ORIGIN      
        1 cacgccgccc tgctcatgcg cgaccaggac gaggccggcg ccatgcgcgc cttgatgcgg
       61 gaaggccgtc tttttgccga tgccttcgag gcgcgcgagg acgcgctccg cctggcgttg
      121 cggctcaagg acgctgacgc gctgcgcgcc atgaaagccg tgccggactg gctggagacc
      181 tctccgcccg cgctccagca ccaggccggg acgctgctgc gcgacccctg gctgcaatgg
      241 cgcggactgc tgctccaccg tctggagcat gtgccctatg gcgcgctcgg aatggccttt
      301 ttcgcctgcc tgatctggta tgcggccttt gtcctgcacg gcggatccgg ctccagacgc
      361 tggctgtggc cggtgccccc cgtcgtggct ggcgtgctca gtgtctggcc cgtgctcacc
      421 gtggcatcct ggcaggagac ggcgaacgga atgtcagccg acgccccttt cccgcatgat
      481 ctttggtatt acgtcgccgg tgtcgggctg cgcgaggagc tggcaaagct ggccgccgcc
      541 gccctgttca tgccctggct gctgtggagc cgcgtgccgg gccgcgcgct gctcaccggc
      601 gccttcgccg gccttggctt cgcccttgag gaaaacatca actactacac cgaattcggc
      661 ggcggagtgg cgctggtccg tcttttgacc gcaaacttcc tgcacgccgc gctcacaggc
      721 atcctcacgc acgcgctcta caccgcgctg cgctcgcgct tcacgcgtgt ggacggcttt
      781 ctcgccgcgc tgccggccgt ggtggccgtg catggcctct atgactacac cgcctcctcc
      841 agcatcggcg gcatggactt cgccgccatg gtaatcctgg cgtttgtcgc ctggcatttt
      901 ctggatgtca tggagcagga ggccgctccc gcgcgccggt gggtctcccc gggctcggtg
      961 gttgtgatcg gagcggcgct gctgatcgcc atcattttca tcagcaccgc actggaaaac
     1021 accggccgcg ccggactcgc cgctgccgca atcgaatgcg tggccgtgct gccgctgatg
     1081 ttcatttact ggcgcaggct gggaggttga tgacgcatgg aacaaacatc cccggtcatt
     1141 ttcaacctcg ccagcctgat ggcgccgcgc gttgtcaacg gatgagcggg ctgcccgcgc
     1201 tgtgtgcagg ccagcctgcc tcatccacca agtctgccgc agccgcgcgc accatgggca
     1261 tcaagttccg ccagtcctcc gcaagacggc gggcaacctc cgaagggcag aagctgcgct
     1321 tcctctctgc cgccagcgcc agcagcgcga ggcgaatttt tgcaggctcg tctctcatgc
     1381 ttttttgcct cgccggcatc tcccactgca atacagcacg cgtccccaat cccgctccca
     1441 ctttttcctc caagtgaatg gacgcgagca gcgagcgcgg atccttgtcg gtagatgttg
     1501 tttgctgact ccgtgcatgc tcaagcgggg gatgtgaatt cacacgggct ccccaccgcc
     1561 tcccactcgg cgcgctcctt ctctacttcg gcgccacggc gacgcgcttt ttcattcgca
     1621 tatctgccga gcacaagccg cattggcggc ttgtcggacg atgcagcggc catgatggcc
     1681 cgcgccgcac gcgctggatc acccggctgg cgcccatcca tgccttcgat caagcgcgca
     1741 aacttgcccg aactggccgc atagtcgcca atttcatgct ccgctttttc gaggctgcgg
     1801 gagatgaatg ccgtacggaa cggccccggc tgcaccagcg tgacgtggat gcccagcggc
     1861 gccagttcca agcgcagact ttcgctgagc atctccagcg cccccttggc ggcgccgtag
     1921 atgccaaagc ccgcatagtt cgagatggcc gcggccgcgc tgatgttgat gatgtgtccg
     1981 gagcgcctgg aacgcagccc tggcagtgcg gctcggatca cattcaacgg gccgtggaag
     2041 ttggtctcca tgcatcgccg gatttgctcc tcgccgcact cctcgacagc accgagcaag
     2101 ccttggcccg cattgttcac gatcacgtca ggcttggacg cggccaccac atcgcgcgcc
     2161 tgcgcctggt cggtgacatc cagtgtcatc acctcgcaca tgctgggctt ttccaggtcg
     2221 gcgatcacgc tcgcatcccg cgcggtggcg atgacccgct ggcccgcctc cagcgcggcc
     2281 tcggcgatgg cgcggccgag acctctggag cagccggtga tgagccatgt tttttgtttc
     2341 atgcagggtt caatctccaa agcgtgaaat tcaggacgga ggcaaaagaa acccacgcca
     2401 gataaggcgc gagcagccag ccggacacgc ggctgacctt gtggaatgac agcagcgtgg
     2461 acaaaatcgc cgcccaaagc gccacgatga ccaccagcgc ccagccaatt tcgcgtgctc
     2521 caaagaagac cggagtccag gccgcgttca ggacaagctg cgccagataa aatccatgag
     2581 gtttcgtcca gcccgcccgc cgccacacca gccacgccgc cacggccatc aaggtgtaaa
     2641 gcagcgtcca caccggaccg aagacccacc cgggtgggtt ccatgcgggt ttgacaagct
     2701 ccgcatacca gtcccccgga cccggaaaaa aggcgctgag cgccggcgcg gcaaaggtgg
     2761 cgaataggaa cgcggccagg acgagtccgc cgcgcaaccg tgaatgggga ggcttcatga
     2821 cggggatgaa tacgccgcgc gtatttcgcc ggcctgcttt tgaatggcgg ctttttgttt
     2881 cccatccaga cgcgcgagat tgcgcacctg catggacatg cggggatttt tccccagcac
     2941 aggctcgtgc cggagcaggt aatcccagta cagagtcgtg aacggacagg ccgaggggcc
     3001 cgtgcttttc gccggatcaa aacggcagcc cgcgcagtgg ttgctcatgc gctggatgta
     3061 tttgcctgag gcgatgtacg gcttgctcgc catcacgccg ccgtccgcgt attgggacat
     3121 gccaagcgtg tttggcagct caacccactc caccgcgtcc acatagaccg ccagatacca
     3181 ctcgtggacg cggcgtggat gcgcgccgta tagcagtgcg aagaggccgg tcaccatcag
     3241 cctctggatg tggtgcgcgt agccgtgacg cagcgtctgt ttgatgaccg cgcgcaggca
     3301 ggccatttca gtttcgccag tccagtaaaa gcccggcaga ggcagctccg cgttcatcgc
     3361 gtttcgttcc aggtagccgg gcatgtgcag ccagtaaatg ccgcgcacat actcgcgcca
     3421 gccgagtatc tgccggatga atccctccac ggattcaaga ggcgcggcgc cgtccctcca
     3481 ggcgcgctcc gccgcctcga tcacctctcg cggatcgagc agcttcaagt tcagcgccgc
     3541 gctcagccgg gagtggaaca gccacggctc gccctcccac atcgcgtcct gataacggcc
     3601 aaaatccgcc agcctgcgcg cgatgaagtc ctccagcgcc aggcccgcct ccgccgctgt
     3661 caccggccag tcgaagtcat ccagcgtgcc gggatgcgcg tggaaggtgt cattgaccag
     3721 agcgatgaca ccgcgtgtga tcgagtcagg ctcgaacttg cgcggcggca cgcgcagtcc
     3781 ggggccgcac ctgccgaagg atgcgcggtt gtcatggtcg tagttccacc gccctcccac
     3841 cggcttgcct tcctgcatca gcactcccgt cttctgccgc atctcgcggt aaaagaactc
     3901 catgcgcagt tgtttgcggc cccgcgcatg gcgggcaaac tccgccgtcg tgcaaagaaa
     3961 gtggccgtcc tcgcgcactt cgagcggtgt ccccgcatcc gccgccgcag cctcgatctc
     4021 ccactgcaca ccccactccc ccgcgcgcac catggcgagc ttttccggcc tgctccgcgc
     4081 gatctccgcg cgcagcgcgc tgccgaggga ctcatgcgcg cccatctcgt gataaaacac
     4141 ccgccatcca cgcccgctca aagcctcgcg gaaatgccgc atcgcgctca gaaacatggc
     4201 gatgcgcgcc ttcgtgctcc acaccttcac agactccgcc gccgcctccg ccatccacac
     4261 cgcatcctcc cccgcgttga agccgtcaaa tgcctccgac tccaaatcga gctggtcacc
     4321 gagaacaaga atgaggcgtg agacgcgcgg cataagtgcc actacgcgcg ccggaaggaa
     4381 atggaggcct ccgcctcatc ccgccgcgtc cttcccggtg gcgcgcgcga cctcgaaagc
     4441 gcgaaacctt cccggcgaca tgcccgtgtg gcggctgaaa tggcgggtga aggcgctctg
     4501 gtcgcaccag cccgtctcaa gcgcgatctc gcccaggctt ttgtccgtct cacgcagcag
     4561 ccgaatcgct tcgtcgagtc gcattttgtg cagcaactgc agcggactca tctggaaaag
     4621 ccgccgcatt ttctgctcaa actgatgcat gctcatgccc gcctccctgg cgatttcctc
     4681 aatgcgcagg ctcgtggcaa aatccctgcg catgcggcgc agcgctgcgg acagctcggc
     4741 gaatcctggc gccttgtccc caggcgcgtt caagtcccgc gaaatgccag ccacaccgat
     4801 gacatggccc cgagcgtcgc gcagggcatg cttcgtggtc aggcaccatc ccgccacact
     4861 gcgcgggtag atgtgcatct ccagctggtc gcgcaccgtg cggcctgtcc ttagcacctg
     4921 ctcatcctgg cggctgtaac cggccgccag cgcggcggga aagacctcgg cggggcgctt
     4981 gccgagcagg cgcttcttgt cgccgccggc gcagcggtcc gccagcgtct gattcacgcg
     5041 cgcgtagcgg ccggaggtgt ccttgatgaa aaagaccacg tccggcagct cgtcgaagag
     5101 cgcctcggcg acttcatgcg cggcaaaagg atcattcatc gcggtcattt tgcatgaatt
     5161 ggcagccctc ctgccaaaaa gcaatcaaga cccctccctg acgggcggcc aacctgcgcg
     5221 catgtcccag tcctccatcg aatggtcagg cgtcttcccc gccgtcgtca cccacatgca
     5281 tccggaccaa agcctggacc tgcccgcctg cgcccgccac tgggaggcgc tcattgagtc
     5341 cggcgtctcc gccctcatcg tctgtggttc gctcggcgaa aaccagacgc tgccgcccga
     5401 ggaaaaacgc accgtggtga aacacgccct cgctgtggcc gctggccgcg tgcccgtggt
     5461 ctgcggcgtg gcggagatga gcacgcaggc cgccatccgc tacctgcacg actgcgaaaa
     5521 gctcggcgcc gccggtttca tggtcatgcc gcccatggtc tataagcccg acccgcgcga
     5581 gacgcagcac tggttccgca ccctcgccgc cgccacaccg ctgacctgga tgctttacaa
     5641 caacccgatc ggctaccata ccgatgtcac ccccgagatg ttcgtcgagc ttgctgacat
     5701 cccgaacctg ctcgccatca aagagagcag cgccaacacc cgccgcatca ccgaactgcg
     5761 caatctcacc ggtgaccgct atcgcatctt caccggcgtg gacgatctct tcctggagtc
     5821 cgccatcctc ggcattgacg gctgggtctg tggcagcggc atcgcctttc cgaaagaaaa
     5881 ccagcagctc tgggacctcg cccaggccgg ccgctgggac gaatgccgcg cgctctatcg
     5941 ctggagccag ccgctgatga agctcgacac ccacctgcac ttcgtccagt acatcaaact
     6001 gctctgccag gagaccggcc tcggcaacga atgggtgcgc gaaccgcgcc tgccgctcgc
     6061 cggagccgaa cgcgagcagg tgctcaacat catccgcggt gcgttggcga agaggccggc
     6121 ttgatccgtc gcaacacctc gccgaaaccc gaatgtgaaa cacctgttct tagcgttcat
     6181 cactggcttc ccgttgctct ccgccttggc ggaacgcccg aacgtgctgt tcatcgccgt
     6241 ggacgacatg cgctgcgatc tcggttgcta cggtgtgaag cacgtcttca cgccgaatct
     6301 cgaccgcctc gcggcttccg gcgtgctttt cagccgcgct tattgccagc aagcggtgtg
     6361 caatccttcg cgcgtctcac tcatgaccgg catgcggccg gattcgacca aggtctggca
     6421 tctctcgacc gaaatgcgtt ccgtcatgcc ggacgcggtc acgattcctc agcatttccg
     6481 caagcacggc tactacgcgc ttggcttcgg caaaatcttc cacaatccgt ggccggacaa
     6541 tgtgtcgtgg gacgagccgc atgtgtggcc ggaaaaacca tcgtggtcga aagcggcgac
     6601 cgggaagctt gcggagtttc gcgagcaaat gcgcgccgaa ggcaaaccag aggctcggat
     6661 cgatcgcatg cgcgccgcgg ccactgaggt gctcgacatt cctgacgagg aacacttcga
     6721 tggcgcaatc ggagaacagg ccgtcgccgc catgcgaagg ctcgccgcag aaaagcagcc
     6781 cttctttctc gcggcaggct tcgttcgtcc gcatctgccg ttcgtcgtgc cacggaagta
     6841 ttgggatctc tatgaccgcg cgaagattcc gctcgcgacg aacgaccacc tgccccacgg
     6901 cgctccaagc atggcctttg gcgaattcca gggcggcttc tatgaactgc gcgactacat
     6961 ggactacgcg gacgcccgct ggccgagaga gggaccgctt tcggaagcac aacagcgcga
     7021 actgaagcac ggctactacg cggcggtgtc ctacattgac gcgcaagtcg gccgattgct
     7081 cgatgagctt gatcgtcttg gtctcgccgg caacacgatc gtcgtgctgt ggagcgatca
     7141 cggctggaag ctcggtgagc acggcggctg gtgcaaacag accaactacg agatcgacac
     7201 acgcgcgccg ctgatgatcc gcatgcccgg cgccaaggcg aacggcacca agtcgagtgc
     7261 gctcgtcgaa ttcgtggacg tctatcccac actcgccgac ctggccggcc tgcccgttcc
     7321 tgaaaagctc gaaggcacca gcctcaagcc gctgcttaac ggaaccgcca caaaggtcaa
     7381 agacgccgcc ttcagccagt tcccccgtcg tgaaaaggat ggccaccaca tgggctatgc
     7441 gatgcgcacc gaccgctatc gctacatcga gtggctggag ctttcttcgc ggaagatcac
     7501 cgcgcaagaa ctctacgatc acgagagcga tcccgacgaa aaccagaaca tcgccaccaa
     7561 ggcggaacac gccgaccttc tcgccagcct cagcgcgcag atgtggaaga cgctgccgcg
     7621 tccgaccttt caatggcagg aacccgcgtc aatggcctcc gccgccgcaa cgcccacatc
     7681 gctgccatgg catccggcca ccggcgctgc cttgccgaaa tcggaaccca aaggaccgaa
     7741 gctcgccgtc acttttcaaa atcagagcac ggctgctgtg gaactcatct ggatcagccc
     7801 ccaagggacc gagaagcact acaagacgct tcagccaaaa gatgccttca acatccgcag
     7861 cagcggcggc gccgtgtggg tgatcaaaga tgccagcaac aaacctctcg gtcattttca
     7921 gatccatgga accgcggaca aacctgtgaa agccatcatt cctgcaccat gaaaactttg
     7981 tgcttcatcc tcggaatctt tgtcgctgcc tcgtcggtct gcgtcgccgc atctgagaaa
     8041 cccaacgtcc tcttcatctc ggtggacgat ctcaatgact ggatcggctg cctcggcggt
     8101 catccgcaga cgaagacgcc caactttgac cgacttgcca aaagcggcac gctgttcacc
     8161 aacgcgcatt gcgccgcgcc gcagtgcaac ccgtcgcgca cggccattct caccggcatt
     8221 ccgccctatc gctcgggcgt gtatcacaac ggccagaaga tgcgcgatgt gctgccggat
     8281 gcggagatca tgccgaagtt cttctcccgg cacggctact ggagcgccgg ctccggcaag
     8341 ctgctgcact acttcattga tgccgcttcg tgggacgact acttccccgc caaagaaaaa
     8401 gagaatccgt ttccaccgac cttcgatcct ccgaatcgtc ccatcagcct gcccattgga
     8461 ggtccttggc agtatgtgga aaccgactgg gccgcgctgg aggtgagcga cgaagtctgc
     8521 ggcggcgatt ggagcgtgac gaagtggatt ggcgagcagc tccaacgccc gcacgacaag
     8581 cccttcttcc tcgcctgcgg catctaccgc ccgcatgagc cgtggttcgt gcctaaggag
     8641 tatttcgaac cgttcccgct cgaatccatt cagcccgggc ccggctacaa ggaggatgat
     8701 ctggacgatc ttccgccctc aggacaaaag atgggcccga accgctactt cgagcacatt
     8761 cagaaacagg gacagtggaa gcaggccatc caggcctatc tcgcctccat tcactacgcg
     8821 gatgcgatgc tcggccgtgt gctcgatgcg ctcgaaagcg gcccgcatcg cgacaagacc
     8881 atcgtcgtgc tgtggagcga tcacggctgg catctcggcg agaagcagca ctggcagaag
     8941 tacaccggct ggcgcatctg cacacgcgtg ccgctgatga ttcgcgtgcc cggcggaaaa
     9001 cccggcgcaa tgtgtgatca accggtgagc ctcacggatc ttttccacac cgtcaccgat
     9061 ctttgcggcc ttcccgcacc ggaaggcatc agcaagaaca gcctggtgcc gcttctgcag
     9121 aatccagatg ccgtatggcc gtatgccgct gtcacccaac tcggcgtgcc tgatgaatac
     9181 gccatcagca ccaaggcgtg gcgctacatc cactaccgca acggcgacga ggagctctat
     9241 gacatcgtga acgatcctca cgaatggaac aacctcgcgg cgaaacccga gcacgccgcc
     9301 aaactcaccg agcttcgcgc attcgcgccc aaaaatccgg ttccagtgcc gcaagtgccc
     9361 ttggcatcac ttcccgagct gaaatggcat ccatcccgtg attccgcccc tccctcgaaa
     9421 ccagacggcg gcaccttcga tgtcgtcttc atcaatcagc acacacaacc cgtcgagctg
     9481 tgctggatgt ctcccacagg cgaacccaag tcctatggca tgatccagcc caaggggcag
     9541 aagcgcaagt ccacacggcc gggtgctgtc tggttcatcc gtgatggcag ccagcaaccg
     9601 ctcggccatt tccaaatcgg tgatcgcacg tcgaaagcca tcatccctgc tcaatgattc
     9661 gtattcccat cattgactcc cacaccggtg gcgagcccac gcgagtcgtg ctaggcggcg
     9721 tgacagcgga taacttggaa acgtatcgcc gcgccatcat ctgcgaaccg cgcgggcatg
     9781 aggtgatcgt cggtgcgctg ctcgtggagc cgaaagacgc gagttgcgcg gcgggtgtga
     9841 ttttcttcaa caacgtcggc ctgctcggca tgtgcggaca cggcatgatc ggcctcatcg
     9901 cgacgctgaa gcatctcgga cggatttcag ccggcgagca tcgtgtggag acgccggtgg
     9961 gcatcattac ggccacgctg cacgacgatg gccgcgtgag cattcgcaac gtgccgtcgt
    10021 atcgccaggc gaagggcgtg aaagcgggtg aagtgaccgg agatgtcgcc tggggcggaa
    10081 actggttctt tctcgtctcg gatcacggac tcgatctcac actcaaaaac gtcccgcagc
    10141 tcacgcaggc cagtttggcg atgcgcgagg ccgtgcgagc ggctggatac ccggaagtcg
    10201 atcacgtcga gctttttggc ccgccgcacg atcccgcgaa tcacagccga aacttcgttt
    10261 tgtgccctgg cggcgagtat gaccgctctc cgtgcggcac cggcaccagc gcaaagctcg
    10321 cgtgtctcgc tgctgacgga aaactcgctc ctggcgagac ttggcggcag gaaagcatcc
    10381 tcggcagcgt gtttgaaggc agttaccaaa acggcgaagg tggcatcatc cccacgatca
    10441 ccggcgaagc cttcatcacg gcggaatcga cgttgctgct cgatccggca gacccattcc
    10501 aactcggcat cgcatgaaat ccttactttt cttcactttg agcctttcgg ccttcgccgc
    10561 tgactttgag gcggcgctga gttttccgaa acgcaccgaa aacaaggtct tccgcgaccg
    10621 cgtgcaggcg aactggctgc cggatggcaa aacgttctgg tatcgcgtgc aaacagcgcc
    10681 ggggaagtcg gagtttgtgt tgatcgacgc caacaacggc agccgcaaaa cagccgcgac
    10741 gctcaaacag ctcggtttgc cggaaaaaga ggccgcgaag acctccacga tgaaaattga
    10801 gctgcgacgc acaaggcgca ccggagagtc ctcaggcctg aagttcatca accagctcga
    10861 agaggacgtg gagctctact ggatcaatca ggaaggcggt cacgtgcgct acggcggcgt
    10921 tcgcgcgggc accgagcgcg agcagcatac gttcgacggc catgtgtggc tcatcaccag
    10981 ccgcaccggc gagcatctcg ccgtcgtgga gggcgcggcg caggcgcaga cgttcatcat
    11041 tgatggcaaa ggcatctcga aggccaatac ggaagcatca aaaaagcctg agcgcggttt
    11101 ccgtgcgcca gatggctcgt gcgtcctctt caccgaagcg gacaaagtgg agcgccggaa
    11161 agtgacgatc gtggaatcga cacccaacgg cagcgtgcag cccaagctga aggttttcga
    11221 ctacatcaag cccggcgatc cgctcccaaa gccgcagtgg gtcatcgaaa agagcgatgg
    11281 aacaaagctc cgcgtggcgc gtgatctcta cgagaatccg ttcactgaaa gcggcgaact
    11341 ggatgccgtg tgggcgccgg acagcagcga attttacttt aactacaacc agcgcggcca
    11401 tcagctctac cgcatcctcg ccgtgaatgc gaagaccggc gcggtgcgtg tcgtcgtcga
    11461 ggaaacctcg aaaaccttca ttcactactc tggcaaaagc tggcgtcatt ggctgcacag
    11521 cacgggcgaa ctgctctgga tgagcgagcg ggatggctgg tgtcatcttt ggctctacga
    11581 tgcgaaaaaa agctcgccga agcatcaaat caccaagggc gcgtggccgg tgcgcgaggt
    11641 tttgcaggtg gatgaggcga agaaggagat ctggttcctc gcgagcggtt tgcgagcaaa
    11701 ggaagacccc tatcacacgc atctctgccg cgtgaacttc gacggcagcg gcttccagca
    11761 gctcaccgaa ggcgatggca accaccgcgt cgagttctcg ccgaatcgcg attttttcat
    11821 cgatacatgg tcgcgtgcgg acaagccgcc agtgatggag ctgcgtcgca gccgcgatgg
    11881 cgggctggtg tgcgatttgg agaaagccga ggtctccgcg ctgttcgccg cgg
//
LOCUS       DAOMYJ010000161        11878 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Prosthecobacter sp. isolate AMR_MDS_5629
            NODE_1787_length_11878_cov_21.008543, whole genome shotgun
            sequence.
ACCESSION   DAOMYJ010000161 DAOMYJ010000000
VERSION     DAOMYJ010000161.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867850
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prosthecobacter sp. (wastewater metagenome)
  ORGANISM  Prosthecobacter sp.
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Verrucomicrobiaceae; Prosthecobacter.
REFERENCE   1  (bases 1 to 11878)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11878)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.30
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:34:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,742
            CDSs (total)                      :: 4,684
            Genes (coding)                    :: 4,676
            CDSs (with protein)               :: 4,676
            Genes (RNA)                       :: 58
            tRNAs                             :: 55
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11878
                     /organism="Prosthecobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1787_length_11878_cov_21.008543"
                     /isolate="AMR_MDS_5629"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1965333"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..582)
                     /locus_tag="PLP58_15410"
     CDS             complement(<1..582)
                     /locus_tag="PLP58_15410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009965650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HRJ09965.1"
                     /translation="MSPHPRLQDWISATFGRLTQAQELTLPHILAGRSVLLSSPTGSG
                     KTLAGFLGVLDHLVRAHEAGTLEGRVHCLYVSPLRALTYDIQKNLLPALRGLGLEEVV
                     RLGTRTGDTTASERAKLKRQPPHILLTTPESLAILLPQAGWAEALGGVRFVIVDELHA
                     LAENKRGAHLMLSLERLARRSGILPVSCATGILP"
     gene            complement(756..1343)
                     /locus_tag="PLP58_15415"
     CDS             complement(756..1343)
                     /locus_tag="PLP58_15415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ09966.1"
                     /translation="MSLPEDHPLRKDLPSLGHETALWLQAMRLLRLRLLKRRLDAPLT
                     AFLERWMPRETASETLPEVFELVLEDHLLTSGSAPALADPRWQALLRLPALRAFWVAE
                     LRASHHAHLLKMTPHVWLMDETPLPPGSVIAGLGIPDWSHLPRLAGTGRRFRECGMEN
                     KNRALIEIQPGQTGRVLARYERQGERIVFAGADAG"
     gene            complement(1343..2005)
                     /locus_tag="PLP58_15420"
     CDS             complement(1343..2005)
                     /locus_tag="PLP58_15420"
                     /inference="COORDINATES: protein motif:HMM:NF025729.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4412 domain-containing protein"
                     /protein_id="HRJ09967.1"
                     /translation="MKAILFSLLAGLLAVPSAFADWIMIQKSTTDGQVQEVKIQIKGD
                     KTRLDAGKQMTLIANGGDNGGIVMIMHAQKMKMKLDPEKMKSMMAMAAPALGGDKPAA
                     KPAATGQKEKVGDYECEIYSWSGPIGSGKFWVAADFKGYKEMAAAHDKMMQAMGNPAA
                     AFAPQAADFPGMVIKSEMTVMGKSNISELVSAKEENVDDSVFTIPEDYNEMKIPGLPA
                     GK"
     gene            complement(2069..5119)
                     /locus_tag="PLP58_15425"
     CDS             complement(2069..5119)
                     /locus_tag="PLP58_15425"
                     /inference="COORDINATES: protein motif:HMM:TIGR02604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HRJ09968.1"
                     /translation="MKASNLLLVSFAGLCGLLRAQDGIVTRTTHAKAYGPTNNEVMVD
                     PAKDLPRYPAVEAKDAISTWQVKKGFKLELAACEPLVRDPIALCFDERGRMFVCEMID
                     YSEMRDVTPHLGRVSMLEDKDGDGFYETGTVFADDLPWPTGLIWANGGLYVGATPDIW
                     RFEDKDGDGKAEVREKVFTGFGTGLKIINVQGMMNSFQWGQDNRVHVLAGGGNRGVVT
                     CLKRPDLSGQELGGRDFWFDPLTHEFGLEAGGAQYGMSFDNYGRKFGCSNSDHLQYWL
                     YDDRYAARNPFYDMPPGRRSIAVDGGAAEVFRISPDEPWRIIRTRWRIAGVVKGSVEG
                     GGRVSGYFTGATGTTIYRGDAYGPEFVNNSFSGDAGGQLIHRKIIRYAKDGINLEGER
                     PADERGFEFAASNDTWVRVVNFANAPDGCLHVCDMYREVIEHPWSIPDEIKKHLDLNS
                     GNDRGRIWRIVPDKGVERKGQKVTLASVNTEELVKTLSHTNGWHRDTAQRLLYERQDK
                     AAVPMLKARLAGDSSELEKIHALNALAPLKPEDAGVIEQAVKDPSAQVRERALLLGSQ
                     MRADGRITYATHRLREDESARVRFNYAMLIQKLYDGLPAIAGQTPDGIQADMLKRHGD
                     NPLMLAAILSSAGTDPMRILEVLAASEAPALSDPVTEALLSMIGRQKDEKALAFAIKL
                     LADPGTPLSSRQSGLKALASGLRRSGSSIAKADKDSKLAPVFNEASATVMKTSASESE
                     RVGAVDLLSLATREQAERPLRICIKADQPEKLQSAAVKALNELLGKAAHAPVLAAWSG
                     LKPKARETALAAMLARDDGATALLEAMSERRGPEPKDLAASQVQALAKHKNATIARHA
                     ATVLKSVIPPSREEVARTFAPALSLKGDAKAGQNHYIGRCMACHRAQGLGIEVGPDLI
                     TVKTKGRDALLAAILDPHKEVASQYIAYTVSTREGQTLSGIITQDDASSLTLKMMGGA
                     TVSLQRTAIQGTASAGQSLMPEGLEAGMTPQDMADLLAFIEELH"
     gene            complement(5199..6362)
                     /locus_tag="PLP58_15430"
     CDS             complement(5199..6362)
                     /locus_tag="PLP58_15430"
                     /inference="COORDINATES: protein motif:HMM:NF013630.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HRJ09969.1"
                     /translation="MMPSSTGQYQPGFGTPPKGTPQKTGTALVKAPAKSVPAPSTKAP
                     GSSLEARVSKLEKNDIRQDQRLISLEAGAARSPGGVAGIPASASGKTYVVRPGDTLPE
                     IAARHGTTTAALRAVNHLPGDELYIGQTLDLPTSRPVETAIGGISGIHVVKSGEVFSI
                     LAERYGVSQDALARANPAVYPDKLLVGERLRIPAGARSAPSSSIPKASSPAVSKAVHV
                     VGRGESLGAIAKKYGIPTASLASANKLKNPNIIVPGQRLVIPGGSPSPAAPVPPVAQS
                     MVASAESDLTPLPAAFLPKPAAPEAPATPAKAPAPAPVTAPAYPDAPRQISNSSRGVV
                     AYRLERGDDIHTVANMFSTTEQKIREMNKLPSEKKLKEGDEIVVPALGAVSLN"
     gene            6724..7758
                     /gene="trpD"
                     /locus_tag="PLP58_15435"
     CDS             6724..7758
                     /gene="trpD"
                     /locus_tag="PLP58_15435"
                     /EC_number="2.4.2.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009964313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004048 - anthranilate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate phosphoribosyltransferase"
                     /protein_id="HRJ09970.1"
                     /translation="MKKILSDLKMGNALTEAQIREAAAFLTDEHESDETKAVFLKALA
                     EKGETPAEIAAFVGEFLKLAVDPGLDRARLPGPMLDVVGTGGDKLHLFNVSTTAMFIL
                     AGGGVCVTKHGNRGVTSKAGGADVLEALGIRIDLPVERVARGIETNGLGFFFAPLYHP
                     AFKAVAGARKMLAAEGRRSIFNLLGPLLNPARPDYQLIGVFDPALTRVFGEILLALGR
                     KTAWVVHGKAGQGGMDELSTLGQTQVCAVSGGSLGESVVDPESLGFAKAKLEDLTGGD
                     AAENAAIIEGILSGTEKGPKRDIAVLNAAAGFVITGLAADLAAGCELARTVLDRGAAH
                     AKMRAMRDWC"
     gene            complement(7762..9201)
                     /locus_tag="PLP58_15440"
     CDS             complement(7762..9201)
                     /locus_tag="PLP58_15440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ09971.1"
                     /translation="MSGYIGRAHEREEFRRILAKKKASLVTCQGRRRIGKSRFIAECA
                     TEADHYLSFSGLAPREGISRQEQLDAFTETLAAQTRVPRVRLDSWPAALQLLASQLPP
                     QGSAVVLLDEISWMAAGDADFAGHLKTAWDAHFSKHPHLVLVLCGSVSSWIEENILRS
                     TGFVGRCAWQFRLGPLPLPDCAEFWGRRGARISAADKLRVLSVTGGVPRYLEEIDPAR
                     TAEENIHDWCFNPGGMLFREFDSIFHDLFTRKAGTYREIVRTLVEGPRTLDQISTALG
                     RERGGSLGAALEDLELAGFLRKDAPFDPATGRSRPREARHRLSDNYLRFFLKYVDPQR
                     ERIEKGLFRATALENLEGWESILGLQLENLVLANLDALLPRLKLAHTPLLNAAPYVQR
                     QTQRRKGCQIDLLLRTRRSLYVIEVKLRQNIPASVADEVMEKVVRLKLPPGQSVRTAL
                     VYAGALAPALEESDAFDFLVPVERLFERL"
     gene            9422..10906
                     /locus_tag="PLP58_15445"
     CDS             9422..10906
                     /locus_tag="PLP58_15445"
                     /inference="COORDINATES: protein motif:HMM:NF013237.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent transferase"
                     /protein_id="HRJ09972.1"
                     /translation="MTDLLRHPLWQADTLGTPLPDDDFGVSVSLPLWRHVIGYEEKDP
                     DVVSKFRSGYPRFCCPPAIARLFDAATRELAAAGERCLVFPRVSHARRCVEFISRAGF
                     TGRAEAWLGLGAAVFPEAAHEEARRFWRYCGEVVTTRQAAHALGEQTCPHTEAEGHEA
                     NLTLRQRLAELSGQPVEDVFLFPSGMAANYAVHRMLTTLFPGRKTVQLDFPYVDVLKL
                     QQTFGSGAHFLPLVREDEYEDLRVLLEREPVAGIFCEAPSNPMLRCVDFPRLLEIRAA
                     TQTDAPVIVDDTIATVAHADAFRVADVVTSSLTKAFSGVGDVMAGSVILHAASPHHAA
                     FSAFLRAHADHELWRGDAVALEQNSRDFPARATRMSDNALALAEYLAAHPRVEKVWHA
                     REQGGAGYEHIRRADGGYGCLFSFLLKDAATASPRFYDALRVCKGPSLGTHFTLVCPY
                     TLLAHYDELDWAESCGVPRHLLRVSAGLEDSADLIARFDEALRA"
     gene            10957..11631
                     /locus_tag="PLP58_15450"
     CDS             10957..11631
                     /locus_tag="PLP58_15450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018292172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRJ09973.1"
                     /translation="MPEPVLAATDLHRSYALDGESVPALRGVSLEVAAGDFIAVTGPS
                     GCGKSTLLQLCGVMDRADAGTLRLDGRDVSALSDNALTRLRRDRIGFVFQFFNLLPTL
                     SLLENIAMPLLLARQPEKEAFEKARALAERVGIAHRLAHFPAQVSGGEAQRAALARAV
                     VHEPALLIADEPTGSLDSENGRNVLDLFRTLNRDLRIAILLATHDPTVAAAAQRQVRM
                     KDGRIV"
     gene            11680..>11878
                     /locus_tag="PLP58_15455"
     CDS             11680..>11878
                     /locus_tag="PLP58_15455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ09974.1"
                     /translation="MLLPRLILRPLFKEPGRTLLTVLGVTLGVAVMLGIQLANRGSLG
                     GFAAALDAVSGKAALEITSPPL"
BASE COUNT         1913 a         3746 c         3932 g         2287 t
ORIGIN      
        1 gggcaggatg cccgttgcac atgagacagg cagaatgcct gacctgcgcg ccagccgctc
       61 caggctgagc atgaggtgcg cgccgcgctt gttctccgcc agcgcgtgca gctcgtccac
      121 gatgacgaaa cgcacgccgc ccagcgcctc cgcccagccc gcctgcggca aaaggatggc
      181 caggctctcc ggcgtggtga gcaggatgtg cggcggctgg cgcttcaact tggcgcgctc
      241 actggcggtg gtgtcacccg tgcgggtgcc gaggcgcacc acctcctcca ggcccaggcc
      301 gcgcagcgcg ggcagcaagt ttttttggat gtcataggtc agcgctcgca gtggcgaaac
      361 atagaggcaa tgaacgcgcc cctccagcgt ccccgcctcg tgcgcgcgga cgaggtgatc
      421 cagcacgccc aggaaacccg ccagcgtctt gccactgccc gtgggcgagg agagcagcac
      481 cgagcggcct gccaggatgt gcggcagcgt cagctcctgc gcctgagtca gcctgccaaa
      541 agtcgcgctg atccagtctt gcagccgtgg atgcggagac atgatgccag catcatcgga
      601 tgaggtttgg agaaatctta ttttcgcgcc cggcactccc cactttacgc ttgtgccgtg
      661 cttggtaaca acgccgcgat gacaccgggc gcgaaatgcg agcgcttttg tcacaacgac
      721 gaagatcccg agggctgcgg tgaatgcgcg gcatgtcacc cagcgtccgc tccagcgaag
      781 acgatgcgct ctccctgccg ctcataccgt gcgaggacac gaccggtttg gccgggctgg
      841 atttcaatga gcgcgcggtt tttgttttcc atcccgcact cgcggaaccg ccgtcccgtt
      901 ccggccaggc gcggcagatg ggaccagtcc gggatgccga gcccggcgat gacgctgccg
      961 ggcggcagcg gggtttcgtc catgagccac acatgcgggg tcattttcag caagtgcgcg
     1021 tggtggctgg cgcgcaactc cgccacccag aaggcgcgca gcgcgggcag gcggagcagg
     1081 gcctgccagc gcggatcggc aagggcgggc gcggacccgg aggtgagcag atggtcttcg
     1141 aggaccagtt caaagacttc aggcagggtt tcggaggcgg tttcgcgagg catccagcgt
     1201 tcgagaaagg cggtcagggg cgcgtccagc cgacgcttga ggagacgcag ccggagcagc
     1261 ctcatcgcct gaagccagag cgcggtctcg tggcccagtg aaggcaggtc tttgcgcaga
     1321 ggatggtctt ccggcaggga catcacttcc cggcgggcag gccgggtatc ttcatctcat
     1381 tgtagtcttc ggggatcgtg aagacggaat cgtccacgtt ttcctccttg gcggagacga
     1441 gttcggagat gttggacttg cccatgacag tcatctctga cttgatgacc atgccgggga
     1501 agtcagcggc ctgcggggcg aatgcggcgg ctggattgcc cattgcctgc atcatcttgt
     1561 catgggccgc ggccatttcc ttgtatccct tgaagtcggc ggccacccaa aatttgccgc
     1621 tgccgatggg tcctgaccag gagtagattt cgcactcgta atcacccact ttttcttttt
     1681 gaccggtggc cgcgggtttg gctgcgggct tgtctccgcc gagagcgggg gcggccattg
     1741 ccatcatgct tttcatcttc tccgggtcga gcttcatctt catcttctgc gcgtgcatga
     1801 tcatgacgat gccgccgttg tcaccgccat tggcgatcag tgtcatctgt ttgcccgcgt
     1861 cgaggcgggt cttatctcct ttgatctgga ttttcacctc ctggacctgg ccgtcggtgg
     1921 tggatttctg aatcataatc cagtcggcga acgcggaagg cacggcaagt aacccggcga
     1981 ggagggaaaa aaggatggct ttcatggcgc gcggacatta agcctgccgc gcgcgcgcgg
     2041 caatgccgga aatcctcctg acggcaggtc aatgcagctc ctcgatgaag gccaggaggt
     2101 cggccatgtc ctggggggtc atgccggctt cgagcccttc gggcatgagg ctctggccgg
     2161 cggaggcggt gccctggatg gcggtgcgct ggaggctgac ggtggcgccg cccatcattt
     2221 tcagggtgag gctgctggcg tcgtcctggg tgatgatgcc gctgagggtc tggccttccc
     2281 gggtgctgac ggtgtaggcg atgtattggg aggcgacttc tttgtgggga tcgaggatgg
     2341 ctgcgagcag tgcgtcacgg cccttggtct tcacggtgat caagtcgggg ccgacctcga
     2401 tgccgagccc ctgcgctcgg tggcaggcca tgcagcggcc gatgtagtgg ttctgtccgg
     2461 ccttggcgtc gcctttcaag ctgagcgcgg gagcaaaggt gcgcgccact tcctcgcgcg
     2521 agggcgggat cactgatttg agcactgtgg cggcatgccg ggcgatggtg gcgtttttgt
     2581 gcttcgccaa tgcctgcacc tgggaggcgg cgaggtcttt cggttcgggg ccgcgccgct
     2641 cgctcatggc ttcgagcagg gcagtggcgc cgtcatcccg cgcgagcatg gcggcgaggg
     2701 cggtttcacg cgctttgggt ttcaagccgg accaggcggc gagcacgggg gcgtgggcgg
     2761 ccttgccgag cagttcattc agcgctttga ccgccgcgct ctgaagcttc tccggttgat
     2821 cagccttgat gcaaatccgc aggggccgct ctgcctgctc ccgcgtggca agcgaaagca
     2881 aatcaacagc tcccactctt tcggattcac tggcagaagt tttcatcacg gtcgctgaag
     2941 cttcattgaa gaccggcgcc agtttgctgt ctttgtcggc tttggcgatg gaactgccac
     3001 tgcgccgcaa cccggaggcc aaagccttca agccgctctg acgggatgaa agcggggtgc
     3061 caggatcagc cagcagcttg atggcgaagg ccagcgcctt ctcatccttc tgcctgccga
     3121 tcatgctgag cagcgcttcg gtgactggat cactcaaagc cggcgcttct gaagcggcga
     3181 gcacctccag gattcgcatt gggtcggtac ccgcgctact gaggatggcg gcaagcatga
     3241 gcgggttgtc tccatgcctc ttcagcatgt ccgcctgaat gccgtccggt gtctgtccgg
     3301 caatcgccgg cagtccgtcg tagagcttct gaatcagcat ggcgtagttg aagcgcaccc
     3361 gcgctgattc atcctcgcga agccgatggg tcgcataagt gatccgtccg tctgcacgca
     3421 tctgtgagcc aagcagcagt gcccgttccc tcacctgggc tgaaggatct ttgaccgcct
     3481 gctcgatgac tccggcgtcc tccggcttca gaggtgccag ggcattcaac gcatgtatct
     3541 tctccagttc gctggaatca cccgcgagcc gcgctttcag cattggcacc gccgccttgt
     3601 cctgtctctc ataaagcagc cgctgagccg tgtcgcgatg ccagccgttc gtgtggctga
     3661 gggtcttcac cagttcctca gtgttcacgc tagccagcgt gaccttctgg cctttcctct
     3721 ccacgccttt gtccgggacg atgcgccaga tgcggccgcg gtcgttgccg ctgttgaggt
     3781 cgaggtgttt tttgatctcg tccgggatgc tccaggggtg ctcgatgacc tcgcggtaca
     3841 tgtcgcagac gtggaggcag ccgtcggggg cgttggcgaa gttgaccacg cgcacccagg
     3901 tgtcgttgct ggcggcgaac tcgaagccgc gctcgtcggc ggggcgctcg ccctccaggt
     3961 tgatgccgtc tttggcgtaa cgaatgattt tgcggtgaat gagctggccg ccggcgtcgc
     4021 cgctgaagct gttgttcacg aactctggcc cgtaggcgtc gccgcgataa atggtggtgc
     4081 cggtggcgcc ggtaaagtag ccgctgacac ggccgccacc ctcgacactg cctttcacca
     4141 caccggcgat gcgccagcgc gtgcggatga tgcgccaggg ttcgtcgggg ctgatgcgga
     4201 agacctcggc ggctccgcca tccacggcga tgctgcggcg gcccggtggc atgtcgtaga
     4261 agggattgcg cgcggcgtag cggtcgtcgt agagccagta ttggaggtgg tcggagttcg
     4321 agcagccgaa cttccgcccg tagttgtcaa agctcatgcc atattgcgcg ccacccgctt
     4381 cgaggccgaa ctcatgggtg agcgggtcga accagaagtc cctgccgccc agctcctgcc
     4441 ccgaaaggtc tggacgcttg aggcatgtga ccacgccgcg gttgccgccg ccggcgagga
     4501 cgtggacgcg gttgtcctga ccccattgga agctgttcat catgccctgg acgttgatga
     4561 ttttcagccc ggtaccgaag ccggtgaaga ctttctcgcg cacctcggcc ttgccgtcgc
     4621 cgtccttgtc ctcgaaacgc cagatgtccg gcgtagcgcc gacgtagagg ccgccattgg
     4681 cccagatgag gccggtgggc cagggcaggt cgtcggcgaa gacggtgcca gtctcataaa
     4741 agccgtcgcc atccttgtct tcgagcatgg agacgcgtcc gaggtggggt gtcacgtcgc
     4801 gcatttcgga gtagtcaatc atctcgcaga cgaacatgcg cccgcgctcg tcgaagcaga
     4861 gggcgatggg gtcgcgcacg aggggctcgc aggcggcgag ttcgagcttg aagccttttt
     4921 tcacctgcca ggtgctgatg gcgtccttcg cttccacggc ggggtagcgc ggcaggtctt
     4981 tggcggggtc caccatgacc tcgttgttcg tggggccgta ggctttggca tgggtggtcc
     5041 tggtgacaat gccgtcctgc gcgcgcagga ggccgcaaag tccggcgaaa ctgacaagga
     5101 gcaggttgga cgctttcatg aggcggacaa aacgcggctc cgcgggccgg gtcatgcgcc
     5161 cggatgacag cttcccgtat ttttatggcc gggcgttctc agttgaggga aacagcgccg
     5221 agcgcgggca cgacgatttc atccccctct ttgagtttct tttcagaggg gagcttgttc
     5281 atctcgcgga ttttctgctc ggtggtgctg aacatgttcg ccactgtgtg gatgtcatcg
     5341 ccacgctcca gacgataggc cactacgcca cggctggagt tgctgatttg tcgcggcgca
     5401 tcagggtaag cgggcgctgt gaccggcgca ggcgcggggg cttttgcggg ggttgcgggg
     5461 gcctccggcg cggcgggttt cggcaaaaag gcggcgggca gaggggtgag gtcgctttcg
     5521 gcggaggcga ccatgctctg cgcaacgggc ggcacgggag cggccggact tggagagccg
     5581 ccgggaatga cgaggcgctg gccgggaacg atgatgttgg ggtttttgag cttgttggcg
     5641 gaggccaggc tggcggtggg aatgccgtat ttttttgcga tggcgccgag gctttcacca
     5701 cgcccgacga catgcacagc cttgctgaca gccggagatg atgctttggg aatgcttgat
     5761 gatggcgcgg acctggctcc cgccgggatg cgcaggcgct caccgacgag aagtttgtcc
     5821 ggataaacgg cggggttggc gcgtgccagg gcgtcctggc tcacgccata gcgctccgcc
     5881 agaatggaga aaacctcgcc ggatttgacg acatggattc cgctgatccc gccgatggcg
     5941 gtttcgaccg ggcggctggt tggcaagtcg agtgtctggc caatgtacaa ttcgtcaccg
     6001 gggaggtggt tcaccgcgcg cagtgctgcc gtggtggtgc cgtgacgggc ggcgatctcc
     6061 gggagcgtgt cgcctgggcg cacgacataa gtcttgccag aagcgctggc cggaatcccg
     6121 gcgacgcctc ccggtgagcg cgctgccccg gcttccagtg agatgaggcg ctgatcctgg
     6181 cggatgtcat ttttttccaa tttggagaca cgggcttcga ggctggaccc gggcgctttg
     6241 gtggatggcg cggggacgct tttggcggga gctttcacca gggcggtgcc ggttttttga
     6301 ggtgtgccct tgggcggcgt gccaaatccc ggctgatact gcccggtgga ggacggcatc
     6361 atcgggggag ctgcgggtgt tggcggctga taggcagagc ccggctggcg gtaggcgggc
     6421 ggctgaatgg ctcccggtgg cagaccgctg ccagccggag gaatgttttg ggctgacgca
     6481 gaatcactga gacaggcggc caaaacggca gcggaggcat attggaacag agcggtgcgc
     6541 ggaagtgcaa gcgatgagtt catggtgcca ttttaataaa tatggatttc atagcaattt
     6601 atttttcaaa aattttagct tttcttaaat tattggggtt tcggccttcc tgttgcgtaa
     6661 gcgacggcaa gttttcagcc gccatcttgc gccttgagcg gttccccgtc atgatcccac
     6721 gccatgaaaa aaatcctctc cgacctgaaa atgggaaacg ccctcaccga agcccaaatc
     6781 cgagaggcgg cggcttttct caccgatgag cacgagtcgg atgaaacaaa ggccgttttt
     6841 ttgaaggcac tggcagagaa gggcgagacg ccggcggaga tcgcggcctt tgttggggag
     6901 tttctgaaac tggccgtgga ccccggcctg gaccgcgccc ggctgccggg accgatgctg
     6961 gacgtggtgg gcaccggagg ggacaagctg catcttttca atgtctcaac cacggccatg
     7021 ttcattctgg cgggcggcgg cgtgtgcgtg acgaagcacg ggaaccgggg cgtgaccagc
     7081 aaggcgggcg gcgcggatgt gctggaggcg ctgggcatcc gcattgacct gccggtggag
     7141 cgcgtggcgc gcggtatcga gaccaacggg ctgggcttct tcttcgcgcc actctatcac
     7201 cccgccttca aagcggtggc tggcgcgcgc aagatgctcg ccgcggaagg gcggcgctcc
     7261 atttttaatc tgctcggccc gctgctgaac ccggcgcggc cggattacca gctcatcggc
     7321 gtttttgacc cggcgctgac ccgcgtgttt ggcgagatcc tgctcgccct tgggcgcaaa
     7381 accgcgtggg tggtgcatgg aaaggcgggc caaggcggca tggacgagct ttccaccctg
     7441 ggccagacgc aggtgtgcgc ggtgagcggc ggatcgctgg gcgaaagtgt ggtggatcct
     7501 gaatcgctcg gttttgccaa agcgaagctg gaggacttga ccggcggcga cgcggcggaa
     7561 aacgcggcca tcatcgaggg catcctctcc ggcacggaga aggggccaaa gcgcgacatc
     7621 gccgtgctca acgcggcggc gggcttcgtc atcaccggcc tggccgccga cctcgccgcc
     7681 gggtgcgagc tggcgcgcac cgtgctcgac cgcggcgccg cgcacgcaaa gatgcgcgcc
     7741 atgcgggact ggtgctgaga ctcacaagcg ctcgaacagg cgctccacag gcacgagaaa
     7801 gtcgaaggca tcggactcct ccagcgcggg tgccagcgcg cccgcataga ccagcgccgt
     7861 gcgcacagac tggccgggcg gcagcttcag gcggaccacc ttttccatca cctcgtcagc
     7921 cacgctggca gggatgtttt ggcgtaactt cacctcgatg acatagagtg agcggcgtgt
     7981 gcgcaggaga aggtcaatct ggcagccttt tcgccgctgc gtctggcgct gtacatatgg
     8041 cgcggcattc agcaggggcg tatgcgccag cttcaggcgg gggagcagcg catcgaggtt
     8101 ggcgagcaca aggttctcca attgcaggcc gaggatggac tcccagccct ccaggttttc
     8161 gagcgcggtg gcgcggaaga ggcctttctc aatgcgctcc cgctgcgggt ccacatactt
     8221 caaaaagaag cgcagatagt tgtccgaaag ccggtggcga gcctcgcgcg ggcggctgcg
     8281 gccggtggcc gggtcaaagg gcgcgtcctt gcgcagaaag cccgccagct cgaggtcctc
     8341 caaagccgcg cccaggctgc cgccgcgttc gcgccccagc gccgtgctga tctggtccag
     8401 cgtgcgcggc ccctccacga gagtgcgcac gatctcgcgg tatgtgcccg ccttgcgcgt
     8461 gaagagatcg tgaaaaatgc tgtcaaactc gcggaagagc atcccgcccg gattgaagca
     8521 ccagtcgtga atgttctcct ccgccgtgcg cgcggggtct atctcctcca ggtagcgcgg
     8581 caccccgcca gtgacgctga ggacgcgcag cttgtccgcc gcgctgatgc gcgcgccacg
     8641 ccgcccccaa aactccgcgc agtccggcag cggcagcggc cccagccgga actgccacgc
     8701 gcagcgcccc acgaagccgg tgctgcgcag gatgttttcc tcgatccagg aggacaccga
     8761 gccgcacagc acgagcacga ggtgcggatg cttcgagaaa tgcgcgtccc aggccgtctt
     8821 caggtgcccg gcaaagtcgg cgtcccccgc ggccatccag gaaatttcat cgagcagcac
     8881 aaccgcgctg ccttgcggcg gcagttggct ggcaagcaac tgcaaggcgg ccggccagga
     8941 gtccagccgc acacgcggca cccgtgtctg cgccgccagg gtttccgtga aagcatcgag
     9001 ctgctcctgc cggctgatgc cctcgcgcgg tgccaggccg ctgaagctga gataatgatc
     9061 cgcctccgtg gcgcactcgg cgatgaagcg gcttttgcca atgcgtcgcc gcccctggca
     9121 ggtgaccagg gaagccttct tttttgccag gatgcggcgg aattcttccc gctcgtgggc
     9181 gcgtccgatg taaccgctca tgtcgttttc gtgcctgaat gttcgttgcc atttttggca
     9241 ccaaatcaac attttgtcaa ttcggtgcct taatatttat attattactt ggcaccaaat
     9301 caacggtttg tcattttagt gcccaaaata tcacgacctt ttcaggcacc aaaatgacat
     9361 cccgtacgcc cagggaatca cttgagcgcc cttgccaccc cgcgtatctg catggccgct
     9421 catgaccgat ctcctgcgcc atcccctctg gcaagccgac actctgggca cgccgctgcc
     9481 ggacgatgat ttcggcgtga gcgtgtccct gccgctttgg cggcatgtca tcggttatga
     9541 ggaaaaagac cccgacgtgg tctcgaagtt ccgctccggc tacccgcgct tttgctgtcc
     9601 accggccatt gcgcggctct ttgatgcggc gactcgtgag ctggcggcgg cgggagagcg
     9661 ctgtctggtc ttcccgcgcg tcagccatgc acgccgctgt gtggagttca tttcgcgagc
     9721 gggcttcacg gggcgggcgg aggcgtggct gggtctgggc gcggcggttt ttcccgaggc
     9781 ggctcatgag gaggcgcggc ggttttggcg ctactgcggc gaggtggtca ccacgcgcca
     9841 ggcggctcac gctctgggcg agcagacctg cccgcacacg gaagccgaag gccatgaggc
     9901 gaatctcacc ctgcgccagc ggctcgcgga actgtccggc cagccggtgg aggatgtctt
     9961 ccttttcccc tccggcatgg cggcgaacta cgccgtgcat cgcatgctca ccaccctctt
    10021 ccctgggcgg aaaaccgtgc agctcgactt cccgtatgtg gacgtgctga agctccagca
    10081 gaccttcggc agcggcgcgc atttcctgcc gctggtgcgc gaggatgaat acgaggacct
    10141 gcgcgtcctg ctggagcgcg agcccgtggc gggcatcttt tgcgaggcac cgagcaaccc
    10201 catgctgcgc tgcgtggact tcccccggct gctggaaatc cgcgccgcca ctcagacgga
    10261 cgcgcccgtc atcgtggacg acaccatcgc caccgtggcc catgcggatg ccttccgcgt
    10321 ggcggatgtc gtcacctcca gcctcacaaa ggccttctcc ggcgttggcg atgtcatggc
    10381 cggcagcgtc atcctccatg ccgcctctcc tcaccatgct gccttttccg ccttcctccg
    10441 cgctcatgcc gaccatgagc tttggcgcgg agatgccgtg gcgctggagc agaattcgcg
    10501 cgacttcccc gcccgcgcca cgcgcatgag tgacaacgcg ctcgcgctcg ctgaatatct
    10561 cgccgcgcat cccagggtgg aaaaagtctg gcacgcccgc gagcagggcg gcgcgggtta
    10621 cgagcacatc cggcgcgcgg acggcggtta cggctgcctg ttctccttcc tgctcaaaga
    10681 cgccgccacc gccagcccgc gcttttacga cgcgctgcgc gtctgcaaag gccccagcct
    10741 cggcacccac ttcaccctcg tctgccccta caccctgctg gcgcattacg acgagctgga
    10801 ctgggcggag agctgcggcg tgccgcgcca tctcctgcgc gtctccgccg gtctcgaaga
    10861 cagcgccgac ctcatcgcgc gctttgatga ggcgctgcgg gcttgaggcc gcatttttcc
    10921 tcccgctccg ctccgccagc cgttatctca tggcccatgc ccgagcctgt tttagccgcc
    10981 actgacctgc atcgttccta cgccctcgat ggcgaatccg ttcccgcgct gcgcggcgtg
    11041 tccctggagg tggcggcggg ggatttcatc gccgtcacag ggccttcggg ctgcgggaaa
    11101 tccaccctgc tccagctctg cggcgtcatg gaccgcgcgg acgccggcac gctgcggctg
    11161 gacgggcggg atgtgtccgc gctctctgac aacgccctga cccgcctgcg gcgcgaccgc
    11221 atcggcttcg tcttccagtt cttcaatctc ctgcccacgc tctccctgct ggaaaacatc
    11281 gccatgcccc tgctgctggc gcggcagccg gagaaggagg cgtttgaaaa agcccgcgcg
    11341 cttgccgagc gcgtcggcat cgcccaccgg ctggcgcatt tccccgccca ggtctccggc
    11401 ggcgaggccc agcgcgccgc cctggcccgc gccgtggtgc atgagcccgc cctgctcatc
    11461 gccgacgagc ccaccggcag cctcgacagc gaaaacggcc gcaacgtgct cgatctcttc
    11521 cgcaccctga accgcgacct ccgcatcgcc atcctgctcg ccacccacga ccccaccgtc
    11581 gccgccgccg cccagcgcca agtgcgcatg aaagacggcc gcattgtgtg accggctgcc
    11641 cgaaattccg cactccgcat tccccattcc aaaatccccg tgctcctccc ccgcctcatc
    11701 ctgcgccctc tcttcaaaga gccgggccgc accctgctga ccgtgctcgg cgtcacgctc
    11761 ggggtggcgg tgatgctggg catccagctc gcgaaccgcg gctcgctcgg cgggttcgcc
    11821 gccgcgctgg atgccgtgtc gggaaaggcc gcgctggaga tcaccagccc gccgctcg
//
LOCUS       DAOMYJ010000162        11838 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Prosthecobacter sp. isolate AMR_MDS_5629
            NODE_1795_length_11838_cov_21.198167, whole genome shotgun
            sequence.
ACCESSION   DAOMYJ010000162 DAOMYJ010000000
VERSION     DAOMYJ010000162.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867850
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prosthecobacter sp. (wastewater metagenome)
  ORGANISM  Prosthecobacter sp.
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Verrucomicrobiaceae; Prosthecobacter.
REFERENCE   1  (bases 1 to 11838)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11838)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.30
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:34:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,742
            CDSs (total)                      :: 4,684
            Genes (coding)                    :: 4,676
            CDSs (with protein)               :: 4,676
            Genes (RNA)                       :: 58
            tRNAs                             :: 55
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11838
                     /organism="Prosthecobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1795_length_11838_cov_21.198167"
                     /isolate="AMR_MDS_5629"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1965333"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..262
                     /locus_tag="PLP58_15460"
     CDS             <1..262
                     /locus_tag="PLP58_15460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ09975.1"
                     /translation="VPGNPAPGVPPASAAPAPAPQAGSGGAAQTAAKQIPIPEVKPWN
                     PGQAAATPLPAQPKQEKEKSLLMEHGDEALKLARELLKSQTK"
     gene            316..1092
                     /locus_tag="PLP58_15465"
     CDS             316..1092
                     /locus_tag="PLP58_15465"
                     /inference="COORDINATES: protein motif:HMM:NF014460.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRJ09976.1"
                     /translation="MLRALGRHTLSIVHDLGDYALFTTRAFGAVMRTRRLGRRLARSV
                     YEQGARCLPVILVVGLFTGLVLGLQGYYVLNRFGSEGLLGTLVSLTLVRELGPVLAAL
                     MLVGQAGSALAAELGIQRNSEQIAALETMGVGTHGYLVTPRVVSALLVYPMQAALFVC
                     VGLVGGALSGSWLLGLSPGVYWSAVERALEPRDVSQCLTKAAVFGLLTISLCAYHGFN
                     THRLRSATGARAVSASTTRAVVQSSIVVLCADYVITSFFV"
     gene            1089..1898
                     /locus_tag="PLP58_15470"
     CDS             1089..1898
                     /locus_tag="PLP58_15470"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HRJ09977.1"
                     /translation="MSARDSGILLKAEGVHKRFGDHVVLAGANLEVPRGSVVTVLGRS
                     GTGKSVFLKCLAGILTPEAGSVRFDGAALDQGDAAARAEFRRRCGYLFQSNALFDSLT
                     ALENVVLPLEQTTDLPDKEIQERGMEALRQLDLEAHRDRHPAELSGGMQKRLALARAI
                     VTRPELVLFDEPTAGLDPLRRNAVFTMIAKYQRQFGFTAVIVTHDVAEALVASDSVAL
                     LDGGRMRFQGAPAAFSDSDDPVVKGFRDSAAALSETLHALRRGENIESDES"
     gene            1895..2359
                     /gene="mlaD"
                     /locus_tag="PLP58_15475"
     CDS             1895..2359
                     /gene="mlaD"
                     /locus_tag="PLP58_15475"
                     /inference="COORDINATES: protein motif:HMM:TIGR04430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015914 - phospholipid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipid asymmetry maintenance
                     protein MlaD"
                     /protein_id="HRJ09978.1"
                     /translation="MKAPRLELLTGLFVLLGIAAIGWLTVKLGARALVGGDTYVIEAR
                     FDNAGGLNAGSNVVVAGVPVGRVESIRVDEADFSAIATLRVLAGLKLPSDTMASIKTT
                     GLIGDKYVALSPGADESTLSPGERITMTESSVDLESLIGKMAFGSLDKDSPE"
     gene            2356..2940
                     /locus_tag="PLP58_15480"
     CDS             2356..2940
                     /locus_tag="PLP58_15480"
                     /inference="COORDINATES: protein motif:HMM:NF017319.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HRJ09979.1"
                     /translation="MTSRSFLLITAAALLPLPPAFANPAEAQQRLRSAVGEVLAIADG
                     SSSSAAFASKVRPTLERHISFDTMTSRAVGVGWRQFTQEQRKRAVQLFTSVIIRTYTE
                     KFTPGEHPQITYQNAVSPASGRVDVPTRMVYRGSKYNVTYRMENIGGWRIADVVIEGV
                     SLVANYRAQLGPTFSKGGAAAVISSLEQSVSRRS"
     gene            2937..3680
                     /locus_tag="PLP58_15485"
     CDS             2937..3680
                     /locus_tag="PLP58_15485"
                     /inference="COORDINATES: protein motif:HMM:NF016236.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VacJ family lipoprotein"
                     /protein_id="HRJ09980.1"
                     /translation="MKPAAFLRLCLLPAAGMFFCQCAAPKSVATAGAVDELDDYADTE
                     VADPLEPLNRATFKLNDGLYTAVLRPISKGYETVTPKLVRTGLDNFFENAKYPVRLVN
                     SGLQGKFRRAGHETEKFAVNTITGFGGIIKQSDRLPHLANIPAEDTGQTFAKWGIGHG
                     PYLVLPVLGPSSLREAAGYAGDYVLNPVNWGVFQSKDALQYIPQGVNTIRSLPAQLEI
                     YDQARKDAIDPYISVRSTFIQNRDAAAKE"
     gene            complement(3687..6278)
                     /locus_tag="PLP58_15490"
     CDS             complement(3687..6278)
                     /locus_tag="PLP58_15490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009961542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PSD1 and planctomycete cytochrome C
                     domain-containing protein"
                     /protein_id="HRJ09981.1"
                     /translation="MKPLLFASLTSLPALAAETALTFERDVRPIVKAHCTHCHGEEEK
                     PEGGVDLRLRRFMDDLIVPGQPEKSQLVEIIRSGEMPKKGGPMPAGELAVIEQWIAQG
                     AKTAKPEPLALAPGPLITDEDREYWAFQPVRRPQVPEIRNSKSEIRNPVDLFIAAGLE
                     KAGLHMAPEADRRTLIRRVTLDLTGLPPTPEEVEAFVKDTSPLAYERLVDRLLASKSY
                     GERWARHWLDVAGYADSNGYAEADSERPHAWRYRDYVIRSLNDDKPWDEFIQEQLAGD
                     ELAGATHADYQQAVLDPRRTDQLIATAFLRMAPDGTGDTVDDAKLAQNQVIAEQIKIM
                     TSSLMGLTVACAQCHDHRYDPVTQADYFRLRAIFDPAYHWEAWRKPAQRLYSLYTPEE
                     RAKAAGIEKAAQAIEAEAREMSKKFLDEIFEMEILKLPEDEREPYREARATPKDKQTE
                     AQKALIKKHPSALALYSLNLYDQKKQDQVDAKMAEAKKLRETKPAEGFVMALTEVKGQ
                     VPVSKLHNRGDHEQPKQEVGPGELGILAHPMIEPFKPVQVSSGSTGRRLAYAQWLTSG
                     RHPLTARVLVNRFWLNHMGRGIVNTPGDFGRQGELPSHPALLDWLADEFVKGGWKLKP
                     LHRLILLSHAYRQSSVNHASLQADPDNRLYARFKLRRMDAETLRDSVLAATGSLVEAS
                     YGPPSGIGRDPQGRIVTGVDKGTITTHKVEAAGEADHRRSIYVQARRTRPLTVLDTFD
                     APSMVPNCEMRNQSTVAPQSLLLMNDTFILKSARHLAARAMREAPREQEDQIQRLWQL
                     LFASAATGADLAKCRAYLDTQSAALARHQADNPPPKGSPPADPAREALASLCQVLFSS
                     NRFLYIE"
     gene            complement(6341..6652)
                     /locus_tag="PLP58_15495"
     CDS             complement(6341..6652)
                     /locus_tag="PLP58_15495"
                     /inference="COORDINATES: protein motif:HMM:NF024736.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HRJ09982.1"
                     /translation="MSSKTCQPAGHATNLTDTQWALIEPFLFPAGAKRGRGRRREPHA
                     ARACLDAIRHLLKAGCQWSLPPGEFPPKSTVHDAFAAWTAQGLWPRINTALRGRCALR
                     R"
     gene            6664..7140
                     /locus_tag="PLP58_15500"
     CDS             6664..7140
                     /locus_tag="PLP58_15500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ09983.1"
                     /translation="MAPGFTRSSYLPDRLSGLCREASANHRRAECRASSYTDTGLHAN
                     ALGSLLATGYSSRVSAKECATSPITHRFEGLNVFDARRPWLGVYRLDVRLAAKTGQPT
                     ASLKIEGANECDFRNGFTDKGPVRDRGLPSGNHTRYLRTMAASMETKLVFDADPSP"
     gene            7137..7511
                     /locus_tag="PLP58_15505"
     CDS             7137..7511
                     /locus_tag="PLP58_15505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ09984.1"
                     /translation="MMQYRLMQGTNRPWRDVFREAFEKLQHPDGGGMTLNHPTGKLAD
                     YLPMLDFDPRVLGIEVWSQLTSGFGSSRGFYDHSAGPACTFTSFGMTSCALGGAAGDS
                     SSKITTPTVAVATCCSCPSSMP"
     gene            7537..7857
                     /locus_tag="PLP58_15510"
     CDS             7537..7857
                     /locus_tag="PLP58_15510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ09985.1"
                     /translation="MRAYRRGIFFGSVASLAASETGEVIAPYDRSSFRFSRIIVKDDT
                     LHVTLSGNDPKRPNVQIRFITDQDIASIVDAAEVAFLLKRDANGRLEAAFVRVEAFAY
                     PKTH"
     gene            7894..8031
                     /locus_tag="PLP58_15515"
     CDS             7894..8031
                     /locus_tag="PLP58_15515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ09986.1"
                     /translation="MHVHDISQLHDQKALRGPAFASTDPSLRTPIPIVEMIFSQPLRR
                     V"
     gene            complement(8091..9245)
                     /locus_tag="PLP58_15520"
     CDS             complement(8091..9245)
                     /locus_tag="PLP58_15520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009958060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ09987.1"
                     /translation="MEKAHTYRGELVFVDHANRRGSIRVQGSGMFFRNDPHPFAMLPY
                     GIVRYHDAAADLRDIPLGTVMHVRAFLPPDPKISSVPVLPVDNKSKDANHNRGTGIAP
                     AENHVLLLEDEPSHCQREGLVWKLKDLEIKNREGMIIATREPKQGGDAKATEEKLTFD
                     AATRFWRGRERLSIEEYLAEGAWPADGKKSLGGQSVLLGIMWKPTPDGIFTRFHISDI
                     WLDDASLQRAAKQQTEVHNAFIRTRWMPAWIDAVEYGKFGRATVTATLFGGMDESLYA
                     DFKKDVTATMNGVESTLKHSGGAYGPAHMASKGTILAVTKATGEVPLGSSGIQIRFDT
                     DLIIEGIRPGRVVRVRPSNWPLVNLPREEYISDGSNPEDRFPTPTIFPKY"
     gene            complement(9358..10674)
                     /locus_tag="PLP58_15525"
     CDS             complement(9358..10674)
                     /locus_tag="PLP58_15525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009958060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ09988.1"
                     /translation="MAHADEPFRPEAGKFPPLEKAHAYRGELVFVDHANRRGSLRVAG
                     VGQFRRNDPHPFAMLPYGMVRYHGAPADLRDIPLGTVLHVRAFLPPDPKTSAVPVLPV
                     NNKDKDANHNRGAGIFPAENHVLLLEDEPSHCQRKGLVWKLKEVEIKNREGTIIARRE
                     SKQGGAGKADEETLSFDAATRIWRGRESLRIEHLITEGAWPAEGKKALNGQIVMLGIT
                     WKPTLGGVFTRFHISDIWLDDDAMQNSAQHQTEVHKAFMRSRWLPAWVDAVEYGKFGR
                     ATVTATLFGGMDASLYADFKPGITALMNGVENTLKHTEGGSAGPTQMAARGKLIEVRG
                     SSFPPQIQGGPQISSETKSDSEKKSAGLPESVGNLNSANVPLGSSGIQIRFETDLITE
                     GMRPTRIVKVRPASWPDVHLPREEHLGNGASDIDERFPKPDIFPKY"
     gene            complement(10722..>11838)
                     /locus_tag="PLP58_15530"
     CDS             complement(10722..>11838)
                     /locus_tag="PLP58_15530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009958058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF1552 domain-containing protein"
                     /protein_id="HRJ09989.1"
                     /translation="TPISGLHHPGALSHHHNCISVWLTGGKLGPSDRNTISVDQKMAE
                     MTAPHTRYPSMEVALTQGSLAWTADGVQLPALRRCSEIFASLFEEPKGGKDAQRRALR
                     RKGSVLDANLAEVRQLEKKMGAEDKGRMEQYLTSVREAEIRTRRADAWLDTPLPAISD
                     ADRKRTDREIAQTMAGDYFRAVYDLMVLAFQTDVTRVATFSLGGEGDAFSIPEIGITE
                     SRHQLSHHGGDAGYMEKLTNYDTFAIEQFSYFLTRLSETKDLNGKPLLGTTMALFGSG
                     MSYGHSHGNANLPLVLAGGGDLGLKHGSHLDFNKDAAAFAGYSLGADGALTTAHYQVC
                     SRPVNTDAHMSNLLLLIAQRMGVETDTFGDSNKIVAL"
BASE COUNT         2031 a         3683 c         3829 g         2295 t
ORIGIN      
        1 cgtgccaggc aaccccgctc ccggcgtccc gcccgcatca gcggcgcccg ccccggctcc
       61 acaggctggc agcggcggtg ccgcgcagac tgccgcaaag cagataccca ttcccgaggt
      121 caaaccctgg aatccaggcc aggccgccgc cacaccgctt cctgcccagc caaagcagga
      181 gaaggaaaaa tccctgctca tggagcatgg agacgaggcc ttgaagctcg cgcgcgagct
      241 gctcaagtcg cagacgaagt gaggcgggtt gcgtcattgc aatggacggc gcggtgcgcc
      301 agagtacgcc ctcacatgtt gcgcgcgctt ggcagacaca cgctttcgat tgtccatgat
      361 cttggggatt acgccctgtt cacgacgcgc gccttcggag cggtgatgcg gacgcggcgg
      421 ctcggcaggc ggctggcccg ctccgtttac gagcagggcg cgcgctgcct gccggtgatt
      481 ctggttgtgg gtcttttcac agggctggtg ctcggcttgc agggatatta tgtcttgaac
      541 cggttcggtt cggaagggct gctgggcacc ctggtatcgc tgaccctggt gcgggagctg
      601 gggcctgtgc tggccgcgct gatgctcgtg gggcaggccg ggtcggcgct ggcggcggag
      661 ctgggaatcc agcggaactc ggagcagatc gccgcgctgg agacgatggg cgtgggcacg
      721 cacggttacc tggtcacgcc gcgcgtggtt tcggctttgc tggtttaccc gatgcaggcg
      781 gcattgtttg tctgtgtcgg actggtgggc ggcgcgctct caggctcgtg gctgctgggc
      841 ctttcgccgg gggtttactg gtccgccgtg gagcgcgcgc tggagccccg ggatgtctcc
      901 cagtgcctca ccaaggcggc ggttttcggc cttctcacca tttctctctg cgcctaccac
      961 ggcttcaaca cgcaccggct gcgctctgcg acgggggcgc gcgcggtgag cgcctccacc
     1021 acgcgcgccg tggtccagtc cagcatcgtg gtgctgtgcg cggactacgt catcacctcc
     1081 ttctttgtgt gagcgcgcgc gattccggga ttctgctcaa ggccgaaggc gttcacaaac
     1141 gcttcggcga ccatgttgtg ctcgcgggcg cgaatctgga ggtgccgcgc ggcagcgtgg
     1201 tgacggtgct ggggcgcagc ggcacgggga agtcggtctt tttgaaatgc cttgccggca
     1261 tcctgacccc tgaggctggt agtgtgcgct tcgacggcgc ggcgctcgac cagggtgatg
     1321 ccgccgcgcg cgcggaattc cgcaggcgct gcggctatct ctttcaaagc aacgcacttt
     1381 tcgactcgct caccgccttg gaaaatgtgg tgctgcccct ggagcaaacc acggacttgc
     1441 cggacaagga gattcaagag cgtggcatgg aggctctgcg gcagctcgat ctggaggcgc
     1501 accgtgaccg tcaccccgcc gagctttccg ggggcatgca aaagcgcctg gccctggcgc
     1561 gcgccatcgt cacgcgtccg gagcttgtgc tttttgacga gccgaccgcc gggctggatc
     1621 cccttcggcg gaacgctgtg ttcaccatga tcgccaaata ccagcggcag ttcggcttca
     1681 ccgccgtcat cgtcacgcac gacgtggccg aggcgctcgt ggccagcgac agcgtggcgc
     1741 tgctggatgg cgggaggatg cgcttccagg gcgcgcccgc cgcgttcagc gactcggatg
     1801 atccggtggt gaaaggtttc cgcgacagcg ccgccgcttt gagtgaaact ctgcatgccc
     1861 tgcgccgggg cgaaaacatt gaatccgacg aatcatgaaa gcacccagac tggaactcct
     1921 caccggcctc tttgttctgc tcggcatcgc cgccatcggc tggctgacgg tgaagctcgg
     1981 cgcccgcgcc ctggtgggtg gtgacaccta tgtgatcgag gcgcgctttg acaacgcggg
     2041 cgggctgaac gcgggcagca atgtggtcgt cgcgggagtt cccgtcggca gggtggagtc
     2101 cattcgcgtg gatgaggcgg acttcagcgc catcgccact ctgcgcgtgc tggcggggct
     2161 gaaacttccc tcggacacga tggcctccat caaaacaacc gggctgatcg gtgataaata
     2221 tgtggcgctt tcacctggag cggatgagag tacactaagt cccggtgagc ggatcacgat
     2281 gacggagtcc tccgttgatc ttgaatcgct catcgggaag atggcttttg gcagcctcga
     2341 caaagactct cccgaatgac ttcccgctcc tttctcctga tcacggcagc cgccctgctg
     2401 ccgctcccgc ccgccttcgc caaccctgcc gaagcgcagc agcgcctgcg ctccgccgtg
     2461 ggtgaggtgc ttgccatcgc cgatggatct tcgagcagcg ccgccttcgc gtcgaaagtc
     2521 cggccgacac tggagcggca catcagcttt gacacgatga ccagccgtgc ggtgggcgtc
     2581 ggctggcggc agttcaccca ggagcagcgg aagcgggcgg tgcagctctt caccagcgtc
     2641 atcatccgca cctacacgga aaaattcaca cccggcgagc atccccagat cacctatcaa
     2701 aacgcggtct cgccggcttc cggacgggtg gacgtgccca cgcgcatggt ctatcgcggc
     2761 agcaaataca acgtcaccta ccgcatggaa aacatcggcg gctggcgcat cgcggacgtg
     2821 gtcatcgagg gtgtcagtct ggtggccaac taccgcgcgc aactcggccc cactttcagc
     2881 aaaggcggcg cggccgccgt catcagctct ctcgaacaat ccgtctcccg ccgctcatga
     2941 agcccgccgc tttcctccgc ctctgcctgc tgcccgccgc aggcatgttt ttttgccagt
     3001 gcgccgcgcc aaaatccgtc gccactgccg gtgctgtgga cgagctggat gactacgcgg
     3061 acaccgaggt cgccgatccg ctcgaaccgc tgaaccgcgc caccttcaag ctcaatgacg
     3121 gcctttacac cgctgttctc cgcccgatct ccaaaggcta cgagacggtg acgcccaagc
     3181 ttgtgcgcac ggggctggat aattttttcg agaatgccaa ataccccgtg cgtctggtca
     3241 acagcggcct ccagggcaag ttccgccgcg caggtcatga gacggagaag ttcgccgtga
     3301 acacgatcac cggctttgga ggcatcatca aacagtccga ccgcctgccg catctggcga
     3361 acattcccgc cgaggacacc ggccagacat ttgccaaatg gggcatcggc cacggccctt
     3421 atctcgtgct ccccgtgctc ggtcccagca gcctgcgcga agccgccggc tatgcgggcg
     3481 attatgtgct caatcccgtg aactggggtg tgtttcaaag caaggacgcc ctgcaataca
     3541 ttccgcaagg cgtgaacacc atccgctccc tgcccgcgca gctcgaaatc tacgatcagg
     3601 cgcgcaaaga cgccattgac ccctacattt ccgttcgcag caccttcatt cagaaccgcg
     3661 acgcggccgc caaggagtga tccgggtcac tcgatgtaaa ggaaccggtt tgaactgaaa
     3721 agcacctggc aaaggcttgc gagagcttcg cgggccgggt cggcgggcgg gctgcctttc
     3781 ggcggcgggt tgtctgcttg atgccgggcg agcgcggccg actgggtgtc gaggtaggcg
     3841 cggcatttgg ccagatcagc cccagtggca gcgctggcaa agagaagctg ccacaggcgc
     3901 tggatctggt cttcctgttc ccgcggagcc tcgcgcatgg cgcgcgcggc caggtggcgg
     3961 gcgctcttca ggatgaaggt gtcattcatc aaaagcaggg actgcggcgc cacggtgctt
     4021 tgattgcgca tctcgcagtt cggcaccatg gagggcgcgt cgaaggtgtc gagcacggtc
     4081 agcgggcggg tgcggcgcgc ctgcacatag atgctgcggc ggtggtccgc ctcgccggcg
     4141 gcctccacct tgtgggtggt gatggtgccc ttgtccacgc cggtgacgat gcgcccctgg
     4201 ggatcacgcc cgatgccgct gggcgggccg tagctggcct ccacgaggct gccggtggcg
     4261 gccagcacgg agtcccgcag cgtctcggca tccatgcgcc ggagcttgaa gcgggcgtag
     4321 aggcggttgt cgggatcggc ctggagggag gcgtgattca cggaggactg gcggtaggcg
     4381 tggctgagca ggatgagccg gtgcagcggc ttcagcttcc agcccccttt cacgaactca
     4441 tcggccagcc agtcgagcag cgccgggtgg gagggcagct cgccctggcg gccgaagtca
     4501 cccggcgtgt tcacgatgcc gcgtcccatg tggtttaacc aaaagcggtt caccagcacg
     4561 cgcgccgtga gcgggtgcct gccgctggtg agccactgcg cgtaggcgag ccggcggccc
     4621 gtggagccgc tgctgacctg cacgggtttg aaaggctcga tcatcggatg cgccaggatg
     4681 ccgagctcgc cggggccgac ctcctgcttc ggctgctcgt ggtcgccccg gttgtgcagc
     4741 ttcgacacgg gcacctggcc tttgacctcc gtgagggcca tgacaaaacc ctccgccggc
     4801 ttggtctcgc gcagcttttt ggcctcggcc atcttcgcgt ccacctggtc ctgcttcttc
     4861 tggtcgtaga ggttcagcga gtagagggcc agcgcggagg gatgcttttt gatgagcgct
     4921 ttctgcgcct cggtctgctt gtccttcggc gtggcgcgcg cctcacggta aggctcgcgc
     4981 tcgtcctcgg gcagcttgag gatttccatc tcgaagatct cgtcgaggaa cttcttcgac
     5041 atctcgcgcg cctccgcctc gatggcttgc gcggcctttt cgatcccggc tgcctttgcc
     5101 ctctcctcgg gagtgtaaag ggagtaaaga cgctgcgccg gcttccgcca ggcctcccag
     5161 tggtaggcgg ggtcgaagat ggcgcgcagg cggaaatagt cggcctgggt caccggatca
     5221 tagcggtggt catggcactg cgcgcaggcc acggtgaggc ccatgagcga ggaggtcatg
     5281 attttgatct gctcggcaat gacctggttc tgcgccagct tcgcgtcatc caccgtgtcg
     5341 cctgtgccat cgggtgccat gcgcagaaaa gcggtggcga tgagctggtc cgtgcgccgg
     5401 ggatcaagca cggcctgctg gtaatccgcg tgagtggccc ccgccagctc atccccggcg
     5461 agttgctcct ggatgaactc atcccagggc ttgtcgtcat tgagcgagcg gatcacataa
     5521 tcgcggtagc gccacgcatg aggccgctcg gagtccgcct ccgcgtagcc gttcgagtcg
     5581 gcgtagcccg ccacatccag ccaatgccgc gcccagcgct cgccatagct tttcgaggcc
     5641 agcaggcggt ccacgaggcg ctcataggcc agaggcgagg tgtctttgac aaaagcctcc
     5701 acctcctccg gtgtcggtgg caggccggtg aggtccagcg tcacccgccg gatcaaagtg
     5761 cgccggtcgg cctcgggcgc catgtgcagg cccgctttct ccagcccggc ggcaatgaaa
     5821 agatccacgg gatttcgaat ctcggatttc gaatttcgga tttccggcac ctgcgggcgc
     5881 cgcacgggtt ggaaggccca atactcgcgg tcctcatcgg tgatgagcgg tcctggggcc
     5941 agcgcgagcg gctcgggctt cgcggtcttc gcaccctggg cgatccattg ctcaatgacg
     6001 gccagctccc ccgccggcat ggggccgccc tttttcggca tctcgccgga gcggatgatc
     6061 tccaccagct ggcttttctc cggctggccg ggcacgatga gatcgtccat gaaccggcgc
     6121 aggcgcaggt ccaccccgcc ttcgggtttt tcctcctcgc catggcaatg cgtgcagtgc
     6181 gccttcacaa tcgggcgcac atcgcgctca aaagtgagcg cggtttcagc ggcgagcgcc
     6241 ggcagggaag tgagggaggc aaaaaggagc ggcttcatca gcggcccaaa cctacggcgg
     6301 cggggcgggg gatgttgcca gcgctgggcg cggcgttttt ttagcgtctc aacgcgcacc
     6361 gcccgcgcag ggcagtgttg atgcgcggcc acagaccttg cgcggtccag gccgcaaagg
     6421 cgtcatgcac ggtgcttttg ggcgggaact ccccaggcgg cagcgaccac tggcagccag
     6481 ccttgaggag gtggcggatc gcatcaaggc aggcgcgcgc ggcgtgcggc tcacggcgcc
     6541 gcccgcgccc gcgtttggct cctgcgggga agaggaaggg ttcaatcaac gcccattgag
     6601 tgtctgtgag gttggtggcg tggccggcgg gttggcacgt cttgctggac atgcttcgcc
     6661 accatggcac ccgggtttac tcgttccagc tatttgccgg ataggctctc aggactatgc
     6721 cgcgaagcat ccgcaaatca tcgccgtgca gaatgccggg catcttccta caccgacacg
     6781 ggcctgcatg ctaacgcgct cggcagcctg ctagccacgg gttacagcag cagagtgagc
     6841 gcgaaggagt gcgcgacctc gcccatcaca catcgctttg aaggtttgaa tgtgtttgat
     6901 gcgcggagac cctggctcgg cgtgtatcgg ctggacgtgc ggctcgccgc gaaaactggc
     6961 caaccgacgg ccagtctcaa gatcgaaggc gcgaacgagt gcgactttcg caatggcttt
     7021 accgacaaag gccccgtgcg cgaccgcggc ttgccgtcgg gcaatcacac gcgctacctg
     7081 cgcaccatgg cagcgagcat ggagacaaag ctcgtgtttg atgccgaccc gtcaccgtga
     7141 tgcagtatcg cctcatgcaa ggcacgaacc gcccgtggcg cgatgtgttc cgcgaagcat
     7201 ttgaaaagct gcaacatccc gacggcggcg gcatgacgct gaaccatccg acgggcaagc
     7261 tcgccgacta cctgccgatg ctcgacttcg atccgcgcgt gctcggcatc gaggtgtgga
     7321 gccagctcac ctccggcttt ggctccagtc gcggcttcta cgatcacagc gcgggcccag
     7381 cctgcacatt tacaagcttt gggatgacat cctgcgcact gggcggcgct gctggggatt
     7441 cttcgtcaaa gatcacaaca cctacggtcg cggtcgcaac gtgctgctcg tgcccaagct
     7501 cgatgccatg acctccaccg agcgcgaaca gcggctttgc gtgcttaccg ccgcggcatc
     7561 ttctttggct ccgtcgcctc actcgccgcc agcgagaccg gtgaagtcat cgcgccgtat
     7621 gatcgcagca gttttcgctt cagccgcatc atcgtgaaag acgacactct gcacgtcacc
     7681 ttatcgggca acgatccgaa acgcccgaac gtgcagatcc gcttcatcac cgatcaagac
     7741 atcgccagca tcgtggatgc cgctgaggtc gcgtttctgc tgaaacgcga cgcaaacggc
     7801 cggcttgagg ccgcctttgt gcgcgtggaa gccttcgctt atccgaagac gcactagcgc
     7861 ggccagcccc tcaccgccga aatgctgcgc ggcatgcatg tgcatgacat ctcgcagctt
     7921 cacgatcaaa aagccctgcg cggccccgcc tttgccagca ccgatccctc cctgcgcacc
     7981 ccaattccca tcgtggaaat gatcttctcc cagccgctgc ggcgtgtgtg aaattctttg
     8041 gcccatcttg aatcagctat gtgttgccgt gattgaatgc ggtatgcagc tcaatacttc
     8101 ggaaagatcg tgggcgtggg gaagcggtct tcggggttgc tgccgtcgct gatgtattct
     8161 tcgcggggga gattgacgag gggccagttg gaagggcgga ctcgcacgac acggccgggg
     8221 cggatgcctt cgatgatgag gtcggtgtcg aaacgaatct gaatgccgct gctgccaagc
     8281 gggacttcgc cggtggcttt ggtgacggcg aggatggtgc ccttcgaggc catatgagcg
     8341 ggaccgtagg ctccgcctga atgcttcagc gtgctttcga cgccgttcat ggtggcggtg
     8401 acatccttct taaagtcggc gtagagcgat tcatccatgc cgccgaacaa ggtggcggtc
     8461 acggtggcgc ggccgaactt gccatactcg acggcgtcga tccacgcggg catccagcgc
     8521 gtgcggatga aggcgttgtg cacttcggtc tgctgcttgg cggcgcgttg cagcgaggcg
     8581 tcgtcgagcc agatgtccga gatgtgaaag cgggtgaaga tgccatcggg cgtgggtttc
     8641 cacatgatgc cgagcagcac gctctggccg ccgagtgatt ttttgccatc ggcaggccag
     8701 gcgccttcgg cgaggtattc ttcgatgctg aggcgctcgc gaccgcgcca gaatcgcgtg
     8761 gcggcatcga aagtgagctt ttcctcggtc gctttggcat cgccgccctg cttgggctcg
     8821 cgcgtggcga tgatcatgcc ttcgcggttc ttgatctcca gatctttgag cttccagacg
     8881 agtccttcgc gctggcaatg gctcggttcg tcttcgagca gcaaaacatg attctccgcc
     8941 ggtgcaatgc cggtgccgcg gttgtggttg gcgtccttgc ttttgttatc gaccggcaaa
     9001 accggcacgg aggaaatctt cgggtcgggc gggagaaagg cgcgaacgtg catcaccgtg
     9061 ccgagcggga tgtcccgcag atccgccgcc gcatcgtgat aacgaacgat gccatacggc
     9121 agcatcgcga aaggatgcgg gtcattacgg aaaaacatgc ccgatccctg cacgcgaatg
     9181 cttccgcgac ggttcgcatg atcgacgaag acgagttcgc cgcgatacgt gtgcgctttt
     9241 tccaacgacg ggaactttcc agtttcagga cgaaatggct cgtcaccggc gaacgcagcg
     9301 caaaagccca caacgaagaa cgaggaacaa agaacaaaga acggtttcat aacaggatca
     9361 gtatttgggg aagatgtcgg gcttggggaa gcgctcgtcg atgtcggagg ctccattgcc
     9421 gagatgctct tcgcggggca gatggacatc cggccagctc gcggggcgga ctttgacgat
     9481 gcgcgtgggg cgcatgcctt cggtgatgag atcggtttca aagcggatct gaatgccgct
     9541 gctgccgagc gggacgtttg ctgagtttag atttcccaca gattcaggga ggcccgcaga
     9601 tttcttttct gagtcagatt ttgtttcaga gctaatctgt gggcctccct gaatctgtgg
     9661 gggaaaactg cttccacgaa cctcgatgag tttgcccctc gccgccattt gcgtgggtcc
     9721 ggcggaaccg ccttcggtat gcttcagcgt gttctcgacg ccgttcatca gcgcggtgat
     9781 gccgggtttg aagtcggcgt agagcgaggc gtccatgccg ccaaagagtg tcgcggtcac
     9841 ggtggcgcgg ccgaacttgc catactcgac cgcatcgacc cacgcgggca gccagcggct
     9901 gcgcatgaag gctttgtgta cctcggtttg atgctgagcg gagttttgca tcgcgtcgtc
     9961 gtccagccag atgtccgaga tgtggaagcg ggtgaacaca ccgccaagcg tgggcttcca
    10021 cgtgatacca agcatcacaa tttggccatt cagagccttt tttccttccg caggccacgc
    10081 gccttcggtg atgaggtgtt cgatgcggag ggattcgcga ccgcgccaga tgcgcgtggc
    10141 agcatcgaag ctcagcgtct cctcgtcggc cttcccagcg ccgccttgct tcgattcgcg
    10201 gcgggcgatg atcgtgcctt cgcggttctt gatctccact tccttgagct tccagacgag
    10261 tcccttgcgc tggcaatggc taggctcgtc ttcgagcagt aggacgtggt tctcagccgg
    10321 gaaaatgccc gctccacgat tgtgattcgc gtccttgtcc ttgttgttca ctggcagcac
    10381 cgggacggcg gaggtctttg gatcgggcgg caaaaacgca cggacatgca gcaccgtgcc
    10441 gagcgggatg tcgcgcaaat ccgccggtgc gccgtgataa cgcaccatgc cataaggcag
    10501 catggcaaac ggatgcggat cgttccgccg aaactggccc acgccagcca cgcgcaggct
    10561 tccgcgtcgg ttcgcatgat cgacgaagac gagttcacca cgatacgcgt gcgctttctc
    10621 caacggcgga aactttcctg cctcggggcg gaagggctca tcggcgtgtg ccatgcacca
    10681 accaagaacc aacaacgacg aaccaagaac aagaaacctg atcacagcgc gacgatcttg
    10741 ttgctatcac caaacgtgtc tgtctccacg cccatgcgct gggctatgag gaggaggagg
    10801 ttgctcatgt gggcgtcggt gttcaccgga cggctacaga cctggtagtg ggcggtggtg
    10861 agggcaccgt cggcaccgag cgagtagcct gcgaatgcgg cggcgtcctt gttgaaatcg
    10921 agatggctgc cgtgcttcag gccgagatcg ccgccgccgg cgagcacgag cgggagattg
    10981 gcattgccat ggctgtggcc gtaggacatg ccgctgccga aaagcgccat cgtggtgccg
    11041 aggagcggtt tgccgttgag gtctttcgtt tcggaaagcc gcgtgaggaa gtagctgaac
    11101 tgctcgatgg caaaggtgtc gtaattcgtg agcttttcca tgtaaccggc atcgccgccg
    11161 tgatggctga gctggtgccg cgactcggtg atgccgatct caggaatgga aaaggcatcg
    11221 ccctcgccac cgaggctgaa ggtggccacg cgggtcacat cggtctgaaa cgcgagcacc
    11281 atgaggtcat acacggcgcg gaaataatcg cccgccatcg tctgtgcgat ttcacggtcc
    11341 gtacgcttgc gatccgcgtc ggaaatggcc ggcagcggtg tatcgagcca ggcatcggcg
    11401 cggcgcgtgc ggatctccgc ctcacgcacg gaggtgaggt attgctccat gcggcctttg
    11461 tcctctgcgc ccatcttttt ctcaagctgg cgcacttcgg cgagatttgc atcgagcacg
    11521 ctgcccttgc gacgcaaggc acggcgctgg gcgtcttttc cgcctttcgg ttcttcaaag
    11581 agcgaggcga aaatctcgct gcaacgccgc aacgcgggca actgcacgcc atccgccgtc
    11641 cacgcgagcg aaccctgcgt cagcgcgacc tccatcgacg gatagcgcgt gtgcggcgcg
    11701 gtcatttccg ccatcttctg gtccaccgag atcgtgttgc gatccgaagg ccccaatttg
    11761 ccgccggtga gccacaccga gatgcagttg tgatgatgac tcagcgctcc cggatgatgc
    11821 aagccgctga tcggcgtg
//
LOCUS       DAOMYJ010000163        11795 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Prosthecobacter sp. isolate AMR_MDS_5629
            NODE_1802_length_11795_cov_21.860988, whole genome shotgun
            sequence.
ACCESSION   DAOMYJ010000163 DAOMYJ010000000
VERSION     DAOMYJ010000163.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867850
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prosthecobacter sp. (wastewater metagenome)
  ORGANISM  Prosthecobacter sp.
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Verrucomicrobiaceae; Prosthecobacter.
REFERENCE   1  (bases 1 to 11795)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11795)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.30
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:34:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,742
            CDSs (total)                      :: 4,684
            Genes (coding)                    :: 4,676
            CDSs (with protein)               :: 4,676
            Genes (RNA)                       :: 58
            tRNAs                             :: 55
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11795
                     /organism="Prosthecobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1802_length_11795_cov_21.860988"
                     /isolate="AMR_MDS_5629"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1965333"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(131..799)
                     /locus_tag="PLP58_15535"
     CDS             complement(131..799)
                     /locus_tag="PLP58_15535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007418612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin"
                     /protein_id="HRJ09990.1"
                     /translation="MKKLLAEALGTFTLVFAGTGAIVINEVSGGAVTHAGIALTFGLV
                     VLAMIHTFGDVSGAHLNPAVTTAFAAAGRFPWRSVPGYLGAQMTGALAASGLLRFLFP
                     QAATLGATLPAGPPVQSFVLELVLTAILMLTILSVSTGGKEKGVTAGIAIGSVIALEA
                     MFAGPVSGASMNPARSLAPALVSGSLEHLWIYLAAPALGALLAIPLCKATRCDGCCPP
                     LHPS"
     gene            complement(813..1286)
                     /locus_tag="PLP58_15540"
     CDS             complement(813..1286)
                     /locus_tag="PLP58_15540"
                     /inference="COORDINATES: protein motif:HMM:NF040413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6428 family protein"
                     /protein_id="HRJ09991.1"
                     /translation="MISLRHFRSLLDRHPDKAFQLRLPGGGSVPESFHITEVGRVRKD
                     FIDCGGTVRTQESCLLQAWLGGDDDHRLATGKLAGIMRKAAPLLPDEDVPLEIEYEDT
                     LISQYRVSEAAVISGAVVLKLEQKHTDCLAREACGVPEKNATPKASACGCGPSCC"
     gene            complement(1302..1685)
                     /locus_tag="PLP58_15545"
     CDS             complement(1302..1685)
                     /locus_tag="PLP58_15545"
                     /inference="COORDINATES: protein motif:HMM:NF033788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HRJ09992.1"
                     /translation="MQAPIAFARAIADETRFRVIHLLHGRVLCVCELAEILELPQSTL
                     SSHLRVIARAGMLDCERRGKWLFYRVKPSFRPLLRVLFKHFQSTPAEDKALASDQRKA
                     LRRIDCRDAGCCPSPAMKTSSPSRK"
     gene            1810..2448
                     /locus_tag="PLP58_15550"
     CDS             1810..2448
                     /locus_tag="PLP58_15550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009964320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HRJ09993.1"
                     /translation="MNTPVLPALEWRYACKVFDPARIIPADTWCALEQSLVLTPSSFG
                     LQPWKFLVIQDKALREELVPHAWSQRQVADCSHLVVFTIPKAVSEDHIDANIRRMVEV
                     RGGTADALAGFRKMVAGFRSGLEARGGLEQWAKLQAYIALGQFMLAAALHEVDTCPME
                     GFIPARFDEILGLDKEGRTTAVLCPAGYRSADDRYAALPKVRFEAADVIEHR"
     gene            2482..3897
                     /locus_tag="PLP58_15555"
     CDS             2482..3897
                     /locus_tag="PLP58_15555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009960829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1501 domain-containing protein"
                     /protein_id="HRJ09994.1"
                     /translation="MDFADTADHALSVRRRAFLTQSAYGLGGMAFASLADAARAGVPG
                     VLRQPHAPVRTKRVIFLCMAGGPSHLETFDWKPKLKELDGKPFPESFTQGQQLAQLQN
                     AELKARGAFCEFKKWGQSGQEISGLFPNIGGMADDLCVIRSMQTEQINHDTAHAFMNT
                     GSIIKGRPSMGSWLLYGLGCDTSDLPGFVVLTSAGKSGQQPVSARQWSAGILPSKYQG
                     IQFQSKGQPVHYLGSPDGVCQSMQRQVVEEVARLNGMFAEQKLDPEIQTRVAQYEMAF
                     KMQASVPELTDMKGEPPHILDMYGVKEPGDGSFASNCLLARRLAERGVRMIQLYHRAW
                     DHHGGIVEGMKNAALDVDRATAGLIRDLKQRGLLEDTLILWGGEFGRTPMGQGSGRDH
                     HILAFNVFLAGGGVKPGMSLGATDELGYRAVQDVVHVHDLHATMLHLLGIDHKRLNVK
                     FQGLDVRLTGVAGRLVKQVLA"
     gene            3911..4690
                     /locus_tag="PLP58_15560"
     CDS             3911..4690
                     /locus_tag="PLP58_15560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ09995.1"
                     /translation="MFTRLLSLLIAAFWVVMTGLFLRDTYFPDHSRFASVPPRLVLDL
                     FLADASAFNNTLHLYKDGIRTGHASFTARRPGDSATGSYAIVGNGTVVLHEQPLLEDG
                     RRPEAAWRLRGELANAEVWRSLELDVRVPVLDLSAVLEWREGQDLPSVDVRRGGEVLM
                     STEMLRSMLAISGAAGLPALLGGMKSQPGAEKAPAITAREGVMDLAGKRRRCYILTAG
                     LLGSEQVRVWFTESGALARMELPQGWQLIEPLMHGLSLDGG"
     gene            complement(4753..6366)
                     /locus_tag="PLP58_15565"
     CDS             complement(4753..6366)
                     /locus_tag="PLP58_15565"
                     /inference="COORDINATES: protein motif:HMM:NF001964.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HRJ09996.1"
                     /translation="MSAASEIARRRTFAIISHPDAGKTTLTEKLLLYGGAVQLAGSVT
                     ARKNQRATTSDWMELEKQRGISVSSTVLQFDYRDCVVNLLDTPGHKDFSEDTYRVLTA
                     VDAAVMVIDAGKGIEAQTLKLFEVCRRRGVPIFTFMNKLDRPARPPLELLDELERVLG
                     IASCPINWPLGDGVDFKGVYDRETRAVHLFERTVGGKFRAPVNVRDIHDDTVRDALPP
                     LVYARVCEELELLEVVGAPFSMDNIRKGQLTPVFFGSAMNNFGVQLMLDRFLDLAPPP
                     APRQVGEEILMPENPAFSGFVFKIQANMNPRHRDHVAFIRIVSGCFQRDMTATNTRTG
                     AKMRLGNSQRLFAQERETVNEAWAGDVVGLVGNYGFQIGDTLSEDPKVRFDEMPRFAP
                     ECFAYLHNENTSKFKRFRDGLDQLLKEGVAQPFELPDAAVRIPLLGAVGPLQFDVLKY
                     RLETEYNAEVRLEFAPWTLVRWLREKNPADKSKSKDPNPRPELAASYGTTLALDSAGQ
                     WVALFSDKVSCGYFETKNAEKFDISPYPF"
     gene            complement(6406..8688)
                     /locus_tag="PLP58_15570"
     CDS             complement(6406..8688)
                     /locus_tag="PLP58_15570"
                     /inference="COORDINATES: protein motif:HMM:NF012668.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ09997.1"
                     /translation="MPSNDSSDFIQADSFTMKGNPNSIAGVQASETFMVTAKGDTALA
                     EGGLQVGFAKYQFDGAAFDKAQKDYDRLASAQSDCNRDINGFINAYQNTTNDLAAARE
                     SVAAANQKLDDHAAGTAVLSGDDLQSAQQELENAQKEVQRLEGDLSGANLETARETLK
                     TAQEAVGAANKKLNDHADGTAVLSEDDLQSAQQELEKAQQELENAQQEVKRLEMGPAG
                     KARKALDAVAKAQGKIPADYPELANAQFSQDDPAKQAADLQDFQSNLNQSALQVGAQV
                     QAMEKVAGPASYLYEEGTPPPPDRSLLSRQVASYEVDKAIGIGVIAPEKFGVDADGKA
                     IGISMQADGAGVTGLYQGKDSYLQVDLSDPNVQRGLYDLEAMDYITGQIDRHAGNMFI
                     DPETGKVTGIDNDLAFPEKSRQQMLQEGADYASKVCAGPPKLMHRDTAEKILNTDPED
                     LRRQLKDMPRPDGVAPLSDKAIDGAVERLKELQAHLRNPKGPIKVVDEFNKKTYEAAI
                     KEQSKDIGMKDLRQAGDEIYNKSANQAKQSYLGSAAMNIRRYENQGLSPRNETGIAQR
                     NSDGLKQLADQNAGKIAQAQQDLQQDGEVLAKVKQGGLKGVAARMQHGSADSAKAKVD
                     STMETIKKLEKQSDRILAMADDKTAAEIAPQLGRKPPAPPAVQQDAQQPKMKVGAALQ
                     QQSGERPKVPSLDLSNVQQKPMVASLGPDMDDKGQAQLNKQKTREFLQGARKDKAETP
                     AIETPDKQAADKPTTAKRKL"
     gene            complement(8773..9384)
                     /locus_tag="PLP58_15575"
     CDS             complement(8773..9384)
                     /locus_tag="PLP58_15575"
                     /inference="COORDINATES: protein motif:HMM:NF013702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HRJ09998.1"
                     /translation="MPPSYRIGHTLFRSLARNFYDFRVVGAEHMRIQGAALIVANHVS
                     FLDPPFIGQAFDEPLYFFARKTLFEHPLMGRVLRSWQTIPIDRDKPDASSLKATIRLL
                     KSGKKVLMFPEGTRSPDGTPQPAEAGVGLFIAKSRAPVLPVRIYGTYEAYPRGAWFLR
                     PSQVTLVVGQPYQPRLEGRPDQGRDLYQSLADEVMEHIRGLCL"
     gene            complement(9424..10065)
                     /gene="cmk"
                     /locus_tag="PLP58_15580"
     CDS             complement(9424..10065)
                     /gene="cmk"
                     /locus_tag="PLP58_15580"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018968986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004127 - cytidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HRJ09999.1"
                     /translation="MNETEVITMDGPAASGKSSVARLVAARLGRIYVNTGNMYRAMTW
                     AVLERGVDPHDADAVRSAAADIRIECPVLDGQTQVSVNGRPLTTTDLNSDAVNAAVSL
                     VARVPEVRERLVSDQRSLTALGPLVMEGRDIGSVVFPGSRLKFYIDASEEVRAARRHA
                     QGHTDRVAERDRMDSTRKSSPLVIPEGAQYIDNSHLTLEQAVESVLGLIADGR"
     gene            complement(10218..11570)
                     /gene="aroA"
                     /locus_tag="PLP58_15585"
     CDS             complement(10218..11570)
                     /gene="aroA"
                     /locus_tag="PLP58_15585"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009963075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003866 - 3-phosphoshikimate
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HRJ10000.1"
                     /translation="MATLKVRKLKSFPVEISVPGDKSISHRAAMFAGMADGTTVIEGF
                     LPSEDCLCSVKAMQAMGAEVEPLEERPGAGLVKLAVTGRGPKLQPPAGPLDCGNSGTT
                     MRLLAGILAGQNFTVEMFGDASLSRRPMKRVADPLRLMGAAIEGQGEKICAPLKITGG
                     ALHPIQYTLPVASAQVKSAVLLAGLFASGKTTVIEPVATRNHTERIMSHFGVKWLREG
                     NAITVYGGQAPRATDIVVPGDISSAAFWMVAAAATPDAELMLRNVGLNSTRTGIINVL
                     LRMGAHITHSETDTAGEPRGNVMVRGGDLSATVIGGAEIPNVIDELPILAVAGALARG
                     TTLIRDARELRVKETDRIAAVAENLRRMGVTVTEHEDGMEIEGGARLRGATISTYHDH
                     RIAMAFAIAGLFAEGETVIEGAECINTSYPGFEHDLALFSETREPGAQIPVLSQAPGK
                     "
     gene            complement(11573..>11795)
                     /locus_tag="PLP58_15590"
     CDS             complement(11573..>11795)
                     /locus_tag="PLP58_15590"
                     /inference="COORDINATES: protein motif:HMM:NF014234.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004665 - prephenate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_function: GO:0008977 - prephenate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0006571 - tyrosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="prephenate dehydrogenase/arogenate dehydrogenase
                     family protein"
                     /protein_id="HRJ10001.1"
                     /translation="DRFRDTTRVAGGDPGLWTGISLANREALLASLRDASSTLTELVE
                     ILSKPDEEALRRLLAEANNLRALLPAARI"
BASE COUNT         1811 a         3553 c         4189 g         2242 t
ORIGIN      
        1 ggaggcatca tgtcaggtga gctgaagcgc cgggagatca cccctgggtg atgcctcgcc
       61 catccctatc ttctccgcat gaattcgtgt ccattcgtgc ccattcgtgg ttcaactgca
      121 tcgttacgga ttaggaaggg tgaaggggcg ggcagcagcc gtcgcagcgg gtggctttgc
      181 acagcgggat ggcgagcagg gcgccgagcg cgggcgcggc gaggtagatc cagaggtgct
      241 ccagggagcc ggagaccagc gcgggggcca gggagcgggc gggattcatg gaggccccgg
      301 agacgggccc ggcaaacatg gcctccagcg cgatgacgga gccgatggcg atgccggcgg
      361 tgacgccttt ttctttgccc cccgtggaga cgctgaggat ggtgagcatg aggatggcgg
      421 tgagcaccag ctccagcaca aagctctgca cgggcggccc ggcgggcagc gtggcgccga
      481 gcgtggcggc ctgcggaaac aggaagcgca gcagcccgct ggcggcgagc gcgccggtca
      541 tctgcgcgcc gaggtagccg ggcacagaac gccaggggaa gcggcctgcg gcggcgaagg
      601 cggtggtgac ggcggggtta agatgcgcgc ctgagacatc gccaaaggtg tggatcatgg
      661 cgagcacgac gagcccgaag gtgagcgcga tgccggcatg ggtgacggct ccgccgctga
      721 cttcgttgat gacgatggcg cctgtgcccg cgaagacgag ggtgaaggtg ccgagggctt
      781 cggcgaggag ttttttcatg gtcggcggcg ggttaacagc aggagggtcc gcaaccgcag
      841 gcggatgctt ttggcgtcgc gttcttttcg ggaacgccgc atgcttccct ggcgaggcag
      901 tccgtgtgct tctgctccag cttcaacacc acggcaccgc tgatgaccgc ggcttctgaa
      961 acgcggtatt gggagatgag cgtgtcctca tattcgatct ccagaggaac atcctcgtcc
     1021 ggcagcaggg gagcggcttt tctcatgatg ccggcgagct tgccggtggc aaggcggtga
     1081 tcgtcgtcac ccccgagcca cgcctgcaga aggcaggact cctgggttcg gaccgtgccg
     1141 ccgcagtcaa tgaaatcctt ccgcacacgg cccacttcgg tgatgtggaa ggactcgggc
     1201 acggagccgc cgccgggcag gcgcaactgg aaggctttgt cgggatgacg gtcgaggagg
     1261 gaacggaaat ggcggagtga gatcatggat gggagcaggg gttattttct ggacgggctg
     1321 gaggttttca tggctggcga ggggcagcat ccggcatcgc ggcagtcaat gcgcctcaaa
     1381 gccttgcgct ggtcgctcgc gagtgccttg tcctcggctg gagtggactg gaagtgcttg
     1441 aacaagaccc gcaacagcgg cctgaaggag ggcttgaccc ggtagaaaag ccatttcccg
     1501 cgccgctcgc agtcgagcat gccggcgcgc gcgatcaccc gaagatggct tgacagtgtg
     1561 gactggggca gttccaggat ttccgccagt tcgcagacgc acaggacacg cccgtggagc
     1621 aggtggatga cgcggaagcg cgtctcgtcg gcgatggccc tggcaaaggc gattggtgct
     1681 tgcattgaga aatgtcgtaa cgacaaatca cgatgcgtca agatctgaat ggcggaattt
     1741 tttccccgcc cccctccgca gtcccggttt gacaatgccg ccgcgccccg tagcggaatg
     1801 cgcatgtcca tgaacacccc cgtcctcccc gctctcgaat ggcgctatgc ctgcaaggtt
     1861 ttcgaccccg cgcggatcat ccccgcggac acttggtgcg cgctggagca gtccctggtg
     1921 ctcacgccct cctccttcgg gctgcagccg tggaagttcc tcgtcatcca ggacaaggcc
     1981 ctgcgcgaag agctggtgcc gcacgcctgg agccagcgcc aggtggcgga ctgctcccac
     2041 ctggtcgttt tcaccatccc caaggccgtc tccgaggacc acattgacgc gaacatccgc
     2101 cgcatggtgg aggtgcgcgg cggcacggct gacgcactgg cgggcttccg caagatggtg
     2161 gccggcttcc gctccggcct ggaggccagg ggggggctgg agcagtgggc caaactccag
     2221 gcctacatcg cgctcggcca gttcatgctg gcggcggcgc tgcatgaggt ggacacctgc
     2281 ccgatggagg gcttcatccc ggcgaggttt gatgagatcc tcggcctcga caaagagggc
     2341 cgcaccaccg ccgtgctctg ccccgccggc taccggagcg cggacgaccg ctacgccgcg
     2401 ctgccgaagg tgcgcttcga ggcggcggat gtcatcgagc atcgttgatt gcgctcccgg
     2461 agtccgggcg tttattgggg catggacttt gccgacaccg ctgatcatgc cctgagcgtg
     2521 cgccgccgcg ctttcctcac gcagtccgcc tacgggcttg gcggcatggc gttcgccagc
     2581 ctggcggatg ccgcccgcgc gggtgtgccc ggcgtcctgc gccagccgca cgcgccggtg
     2641 cggacgaagc gggtcatttt tctctgcatg gcgggcggtc cctcgcacct ggagaccttc
     2701 gactggaagc cgaagctgaa ggagctggac ggcaagccct tccccgagtc cttcacccag
     2761 gggcagcagc tcgcccagct gcagaatgcc gagctgaagg cgcgcggggc cttctgcgag
     2821 ttcaaaaaat ggggccagtc cggccaggag atctccgggc ttttcccgaa catcggcggc
     2881 atggcggacg acttgtgcgt catccgctcc atgcagacgg agcagatcaa ccacgacacc
     2941 gcccacgcct tcatgaacac gggcagcatc atcaaaggcc gaccgagcat gggctcctgg
     3001 ctgctctacg gcctcggctg cgacaccagc gacctgcccg gcttcgtcgt gctgacctcc
     3061 gccggcaagt ccggccagca gcccgtctcg gcccgccagt ggtccgcggg catccttccc
     3121 agcaaatacc agggcatcca gttccagtcc aagggccagc ccgtccacta cctcggcagc
     3181 ccggacggcg tgtgccagag catgcagcgc caggtggtgg aggaggtggc gcggctcaac
     3241 ggcatgttcg cggagcaaaa gctcgaccct gagattcaga cccgcgtcgc ccaatacgag
     3301 atggccttta aaatgcaggc cagcgtgccg gagctaacgg acatgaaagg cgagccgccg
     3361 cacatcctcg acatgtacgg tgtgaaggag ccgggggacg gcagcttcgc ctcgaactgc
     3421 ctgctggccc gccgcctggc cgagcgcggc gtgcgcatga tccagctcta ccaccgcgcc
     3481 tgggatcacc acggcggcat tgtcgagggc atgaaaaacg ccgccctgga tgtggaccgc
     3541 gccaccgccg gcctcatccg cgacctcaag cagcgcggcc tgctggagga cacgctcatc
     3601 ctctggggcg gcgagttcgg ccgcacaccc atgggccagg gcagcgggcg cgaccaccac
     3661 atcctggcct tcaatgtctt cctcgccggc ggcggtgtga agcccggcat gagcctcggc
     3721 gccaccgacg agctcggcta ccgcgccgtg caggacgtgg tccacgtcca cgacctgcac
     3781 gccaccatgc tccacctcct gggcatagac cacaagcgcc tcaatgtgaa gttccaggga
     3841 ctcgatgtcc gcctcaccgg cgtcgccggg cgtctggtca aacaagtgct ggcctgacac
     3901 cccgccgccc atgttcacgc gccttctcag ccttctcatc gcggccttct gggtcgtcat
     3961 gaccggcctt ttcctgcgcg acacgtactt ccccgaccac tcgcgcttcg ccagcgtgcc
     4021 tccgcgtctc gtgctcgatc tctttcttgc cgacgcctcc gctttcaaca acacgctcca
     4081 tctttacaag gacggcatcc gcaccggcca tgcctccttc accgcccgcc gccccggcga
     4141 ctccgccacc ggctcctacg ccatcgtcgg caacggcacc gtggtcctcc acgagcagcc
     4201 cctcctggag gatggccgcc gccccgaagc cgcctggcgt ctgcgcggcg agctcgccaa
     4261 cgccgaggtc tggcgctccc tcgaactcga cgtgcgcgtc cccgtgctgg acttgtccgc
     4321 cgtgctcgaa tggcgcgagg ggcaggacct gccctccgtg gatgtgcgcc gcggcggcga
     4381 ggtgctcatg agcacggaga tgctgcgctc catgctcgcc atcagcggag ccgccggtct
     4441 gcccgccctg ctcggcggca tgaaaagcca gcccggcgcg gaaaaagcgc ccgcgatcac
     4501 cgcgcgcgag ggcgtgatgg acctcgccgg caagcgccgc cgctgctaca tcctcaccgc
     4561 gggtcttctc ggctcggagc aagtgcgcgt ctggttcacg gaatccggcg ccctcgcccg
     4621 catggagctg ccccagggct ggcagctcat cgagccgctc atgcacggcc tgtccctcga
     4681 cggcggttga tcacaccgcc atgcgggacg agtcacagca tcaggtggca tgaggccggg
     4741 cgttggcgcg ggtcaaaaag ggtaggggct gatgtcgaac ttctcggcgt tcttcgtctc
     4801 gaagtagccg cagctcactt tgtcgctgaa gagagcgacc cactggccgg cgctgtcgag
     4861 cgcgagggtg gtgccgtagg aggcggcgag ttcggggcgc gggttggggt ctttgctctt
     4921 ggacttgtcc gccgggttct tttcccgcag ccagcgcacg agtgtccagg gggcgaactc
     4981 gagccgcact tcggcgttgt attcggtctc caggcggtat ttcaagacat cgaactggag
     5041 cggccccacc gcgccgagca gggggatgcg cacggcggcg tcgggcagtt caaagggctg
     5101 cgccacacct tccttgagga gctggtccag gccgtcgcgg aagcgcttga acttggaggt
     5161 gttctcgtta tgcaggtagg cgaagcattc cggcgcgaag cggggcatct cgtcaaagcg
     5221 gaccttgggg tcctccgaga gcgtgtcacc gatctggaag ccgtagttgc cgacgaggcc
     5281 gaccacatcc ccggcccagg cctcgttcac cgtctcgcgc tcctgggcga agaggcgctg
     5341 cgagttgccc aggcgcatct tcgcgccggt gcgtgtgttg gtggcggtca tgtcgcgctg
     5401 gaagcagccg gagacgatgc ggatgaaggc cacatggtcg cggtggcgcg ggttcatgtt
     5461 ggcctggatc ttgaagacga agccgctgaa ggcggggttt tccggcatga ggatctcctc
     5521 gcccacctgg cgcggggcgg gcggcggcgc caggtccagg aagcggtcga gcatcaattg
     5581 cacgccgaag ttgttcatgg cgctgccgaa gaacacgggg gtgagctggc ctttgcggat
     5641 gttgtccatg gagaagggcg cgccgacgac ttccagcagc tccagctcct cgcaaacacg
     5701 ggcatagacg aggggcggca gggcgtcgcg cacggtgtcg tcatggatgt cccgcacgtt
     5761 cacgggggcg cggaatttgc cgccgacggt gcgctcgaag agatgcaccg cgcgcgtctc
     5821 gcggtcatag acgcctttga agtccacgcc gtcgccgagg ggccagttga tggggcagga
     5881 ggcgatgccg agcacgcgct ccagctcgtc gagcagctcc aggggcggcc gcgcggggcg
     5941 gtcgagcttg ttcatgaagg tgaagatggg cacgccgcgg cggcggcaga cctcgaagag
     6001 cttcagggtc tgcgcctcga tgcccttgcc ggcgtcaatg accatgacgg cggcgtccac
     6061 ggcggtgagc acgcggtagg tgtcctcgga gaagtccttg tggccgggcg tgtcgagcag
     6121 gttgaccacg cagtccctgt agtcgaactg gagcacggtg gaggagacgg agatgccgcg
     6181 ctgcttttcc agctccatcc agtcggaggt ggtggcgcgc tggttcttgc gcgcggtgac
     6241 gctgccggca agctggacgg cgccgccata gaggaggagc ttttcggtga gggtggtctt
     6301 cccggcgtcc gggtgggaga tgatggcaaa ggtgcggcgg cgggcgattt ctgaggcggc
     6361 ggacatgaag gggggggagg gggccttcca ggagcgggag tgaatttaga gtttgcgttt
     6421 ggcggtggtt ggcttgtcgg cggcctgctt gtccggggtt tcgatggcgg gggtttcggc
     6481 tttgtccttg cgcgccccct ggaggaactc gcgggtcttt tgcttgttga gttgggcctg
     6541 gcctttgtca tccatgtcgg gtccgagcga ggcgaccatc ggcttttgct gcacgttgct
     6601 caaatcaagg gaggggactt tcgggcgctc gccggattgc tgctggaggg cggctcccac
     6661 tttcattttc ggctgctgcg cgtcctgctg gactgcgggg ggggctggcg gcttgcgtcc
     6721 aagctgcggg gcgatctcgg cggcggtttt gtcgtccgcc atggcaagga tgcggtcgct
     6781 ttgcttctcg agttttttga tggtttccat ggtgctgtcc accttggctt tggccgagtc
     6841 cgcagagccg tgctgcatcc gggcggcgac acctttgagg ccgccctgct tgactttggc
     6901 gagcacctcc ccgtcttgtt gaaggtcttg ctgcgcctgg gcgattttgc cggcgttctg
     6961 gtcggccagt tgtttgaggc cgtcggagtt ccgctgggcg atgccggtct cgttgcgcgg
     7021 actcagaccc tggttttcgt agcggcggat gttcatcgcg gcggagccga gataggattg
     7081 tttggcttgg ttggcgcttt tgttgtaaat ctcgtcgcct gcctggcgga gatccttcat
     7141 gccgatgtcc ttggactgct ccttgatggc ggcctcatag gtttttttgt tgaactcgtc
     7201 caccaccttg atgggcccct tgggattcct gagatgcgcc tgaagctcct tgaggcgctc
     7261 gacggctccg tcaatcgcct tgtcgctcag cggggcgact ccatcggggc gcggcatgtc
     7321 cttgagttgc cgccgcaggt cttcggggtc ggtgttcaag attttttcag cggtgtccct
     7381 gtgcatcagt ttgggcggcc ccgcgcagac tttggaagca tagtcagcgc cttcctgcag
     7441 catttgctgg cgcgactttt caggaaacgc caggtcgttg tcaatgccgg tgacttttcc
     7501 cgtctcggga tctatgaaca tgttccccgc gtgccggtct atctgcccgg tgatgtagtc
     7561 catcgcctca agatcgtaga gaccccgctg cacgtttgga tcggagaggt caacctggag
     7621 gtagctgtcc ttgccctggt aaagcccggt gacaccggcg ccgtccgcct gcatgctgat
     7681 gccgatggcc ttgccatccg cgtccacgcc aaatttttca ggcgcgatga cgccgatgcc
     7741 gatcgctttg tccacctcat aggaggccac ctggcgggag agcagggagc ggtccggcgg
     7801 cggcggtgtg ccctcctcgt aaagatagga ggcgggaccc gcgacttttt ccatggcttg
     7861 gacctgcgct cccacctgca aggcgctttg attgagattg ctttgaaagt cctgcaggtc
     7921 ggccgcctgc ttggcagggt cgtcctggct gaactgcgcg ttggccagtt cggggtagtc
     7981 cgcgggtatt tttccctggg ctttcgccac tgcgtcgagg gctttcctgg ctttgccggc
     8041 cggtcccatt tcaaggcgct tcacttcctg ttgcgcgttt tccagctcct gctgcgcttt
     8101 ttccaactcc tgctgcgcgc tttgcaggtc gtcctcactc aggacggcgg tcccgtcggc
     8161 gtgatcattg agttttttgt tggcggctcc aacagcttcc tgggcggttt tgagggtctc
     8221 cctggcggtt tcgaggtttg cgccagatag atccccttca aggcgctgca cttctttttg
     8281 cgcgttttcc agctcctgct gcgcgctttg cagatcgtcc ccactcagga cggcggttcc
     8341 ggcggcatgg tcatcgagtt tttgattggc ggcggcgacg gactcccggg cggctgcgag
     8401 gtcgttcgtg gtgttttgat aggcattgat gaagccattg atgtcgcggt tgcaatccga
     8461 ctgggctgag gcgaggcggt cgtaatcttt ctgcgccttg tcgaaggcgg ccccgtcgaa
     8521 ctggtacttc gcaaagccca cttgcagtcc gccctccgcc agcgcggtgt ctcccttggc
     8581 ggtgaccatg aaggtctcgc tggcctgcac gcctgcgatg gagttggggt tgcccttcat
     8641 ggtgaatgag tcagcctgga tgaagtcgga ggagtcgttg ctgggcatgg cagttgctgg
     8701 agagttgttt gaattaaccc gcttcaaggc gggtgtgacg atggattttg cgcgcgggcg
     8761 ggcgcggtct tgtcacaaac atagtccgcg aatgtgctcc atgacctcgt cggcgaggga
     8821 ttgataaaga tcacggccct ggtcggggcg tccttcgagc cggggttggt agggctggcc
     8881 gacgacgagg gtgacctggg aggggcgcag aaaccaggct ccgcgcgggt aggcctcgta
     8941 ggtgccatag atgcggacgg gcagcaccgg ggcgcggctt ttggcgatga acaggccgac
     9001 accggcctcg gcgggctggg gggtgccgtc gggactgcgg gtgccctcgg ggaacatgag
     9061 gacttttttg ccgcttttga gcaggcggat ggtggccttg aggctggagg cgtcgggttt
     9121 gtcgcggtca atgggaatgg tctgccaact gcgcaggacc ctgcccatga gcgggtgctc
     9181 gaagagggtc ttgcgcgcga agaagtagag aggctcgtcg aatgcctggc cgatgaaggg
     9241 cgggtcgagg aagctgacgt ggttggcgac gatgagcgcc gcgccctgga tgcgcatgtg
     9301 ctccgcaccg acgacccgga agtcgtagaa gttccgcgca agggagcgga agagggtgtg
     9361 gccgatgcgg taggaggggg gcattgggga atgcggattt cgaattgggg atttcggatt
     9421 tggctatctg ccatcggcta tcaagccgag cacggactcg acggcctgct ccagggtgag
     9481 gtggctgttg tctatgtatt gggcgccttc gggaatgacg aggggggagc ttttgcgggt
     9541 ggagtccatg cggtcgcgct cggcgacgcg gtcggtgtgg ccctgggcat ggcggcgggc
     9601 ggcgcgcact tcctcgctgg cgtctatgta aaacttcagc cggctgccgg gaaagacgac
     9661 gctgccgatg tcgcgccctt ccatgactag cgggccgagg gcggtgaggg agcgctggtc
     9721 gctgacgagg cgctcgcgca cctccgggac gcgggcgacg agggagacgg cggcgttgac
     9781 ggcgtcgctg ttgaggtcgg tggtggtgag cgggcggcca ttgacgctga cctgcgtctg
     9841 cccgtccagc acggggcact cgatgcggat gtccgccgcg gcggagcgca cggcatccgc
     9901 gtcatgcggg tccacgccgc gctccagcac ggcccaggtc atggcgcggt acatgttgcc
     9961 ggtgttgacg taaatgcggc caaggcgggc ggcgacaagg cgcgcgacgc tgcttttgcc
    10021 cgatgcggcg gggccgtcca tggtgatgac ttcggtttcg ttcaaggcgg ggaaggggga
    10081 tggggctggg tttgaaccac tcatcgaaca ctgatgtgac acgaatgggg ggagggtttg
    10141 aagccgctgg ctgatgctgg tttttagaaa tggatggagt ggtgggaact tcgaatttcg
    10201 agcttcgaat tcccagccta cttccccgga gcttgcgaga gaaccggaat ctgggcaccg
    10261 ggctcgcggg tctcggagaa gagggcgagg tcgtgctcga agcctgggta gctggtgttg
    10321 atgcactcgg cgccttcgat gacggtctcg ccttcggcaa agagcccggc gatggcgaat
    10381 gccatggcga tgcggtggtc gtgataggtg gagatggtgg cgccgcgcag cctggcgccg
    10441 ccttcgatct ccatgccgtc ctcgtgctcg gtgacggtga cgcccatgcg gcggaggttt
    10501 tcggccacgg cggcgatgcg gtccgtctcc ttgacgcgca gctcgcgcgc gtccctgatg
    10561 agggtggtgc cgcgggcgag agcgccggcg acggcgagga tgggcagctc gtcaatgacg
    10621 ttggggatct ccgcgccgcc gatgacggtg gcgctcaggt cgccgccgcg caccatgaca
    10681 ttgccgcgcg gctcgccggc ggtgtcggtc tcggagtggg tgatgtgcgc gcccatgcgc
    10741 agaagcacat tgatgatgcc tgtgcgtgtg gagttgaggc cgacattgcg cagcatgagc
    10801 tccgcatcgg gcgtggcggc ggcggccacc atccagaagg cggcgctgga gatgtcaccc
    10861 ggcacgacga tgtccgtggc gcgcggggcc tgccccccat agacggtgat cgcattgccc
    10921 tcgcgcagcc acttgacgcc gaagtgggac atgatgcgct cggtgtggtt gcgggtggcg
    10981 accggctcga tgacggtggt tttcccggac gcaaacaggc cggcgagcag gacggcggac
    11041 ttcacctggg cgctggcgac cggcagggtg tactggatgg ggtgcaaggc gccgccggtg
    11101 atcttgagcg gcgcgcagat tttctcgccc tggccctcga tggcggcgcc catgagccgg
    11161 aggggatcgg cgacgcgctt catcggacgg cgggaaagcg aggcgtcgcc aaacatttca
    11221 acggtgaaat tctgcccggc caggatgccc gccagcaggc gcatggtggt gccggagttg
    11281 ccgcagtcca gggggccggc gggcggctgc aatttcggcc cgcgtccggt gacggcgagc
    11341 ttcacgaggc cggcgccggg gcgctcctcc agcggctcga cctccgcgcc catggcctgc
    11401 atggctttga cggagcagag gcagtcctcg ctgggcagga agccctcgat gacggtggtg
    11461 ccatcggcca tgccggcaaa catggcggcg cggtgggaga tgcttttgtc ccctgggacg
    11521 ctgatttcga cggggaagga tttgagtttg cggactttga gagtggccat gttcagatgc
    11581 gggccgcggg cagcagggcg cggagattgt tggcctcggc gagcaggcgg cggagggctt
    11641 cttcgtccgg cttggagagg atttcaacaa gctctgtcag cgtggaggag gcgtcccgca
    11701 gactggcgag caatgcctcg cggttggcca gggagatgcc cgtccacagg cccgggtcgc
    11761 cacccgccac gcgcgtggtg tcgcggaagc ggtcg
//
LOCUS       DAOMYJ010000164        11793 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Prosthecobacter sp. isolate AMR_MDS_5629
            NODE_1805_length_11793_cov_22.354319, whole genome shotgun
            sequence.
ACCESSION   DAOMYJ010000164 DAOMYJ010000000
VERSION     DAOMYJ010000164.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867850
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prosthecobacter sp. (wastewater metagenome)
  ORGANISM  Prosthecobacter sp.
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Verrucomicrobiaceae; Prosthecobacter.
REFERENCE   1  (bases 1 to 11793)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11793)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.30
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:34:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,742
            CDSs (total)                      :: 4,684
            Genes (coding)                    :: 4,676
            CDSs (with protein)               :: 4,676
            Genes (RNA)                       :: 58
            tRNAs                             :: 55
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11793
                     /organism="Prosthecobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1805_length_11793_cov_22.354319"
                     /isolate="AMR_MDS_5629"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1965333"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..754
                     /locus_tag="PLP58_15595"
     CDS             <1..754
                     /locus_tag="PLP58_15595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009959143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HRJ10002.1"
                     /translation="AQRYRYKNCDMADLEAQLAAADAKGARFKMIATDGVFSMDGTIA
                     PLREICDLAEKHHALVMVDDSHATGFIGRTGRGTHEHCEVMGRVDILTGTLGKALGGA
                     SGGYTSGRKEIIELLRQRSRPYLFSNTLCPSIAGATLEALRLLKTSTRLRDTLEANTR
                     WFRAAMTEAGFNIALGAHPIVPVMLGDAALATRFADAMLERGVYVIGFSFPVVAEGKA
                     RIRTQMSAAHTRADLEKAVAAFAAVRDQLQPG"
     gene            complement(771..1148)
                     /gene="gcvH"
                     /locus_tag="PLP58_15600"
     CDS             complement(771..1148)
                     /gene="gcvH"
                     /locus_tag="PLP58_15600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007416928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein GcvH"
                     /protein_id="HRJ10003.1"
                     /translation="MSQVPAHLRYRDTHEWVDPAQEISPVGITDHAQAELTDVVFVDP
                     PKLGPVTAGQQVCVIESVKAASDIYTPVSGEIVEVNGVLSAEPGLINTDPYGAGWIFK
                     IKPSSPDEAAALMDAAAYTAHIG"
     gene            complement(1193..1789)
                     /gene="leuD"
                     /locus_tag="PLP58_15605"
     CDS             complement(1193..1789)
                     /gene="leuD"
                     /locus_tag="PLP58_15605"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009958269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HRJ10004.1"
                     /translation="MALAKITQVTGTAVHVPGSDIDTDRIIPARFMKCVTFDGLGEFA
                     FHDVRFDKDGKPAGHPLDDPRFKGASILVSGSNFGCGSSREHAPQALYRFGFRAVIAQ
                     SFAEIFFGNCTTLGIPCVCATAEDIARLAAAVEADPRMEVVVDVASRQVRFGAESIPV
                     TVPATAHDALTSGRWDPIGELLEARDAIHSRMTALGWV"
     gene            complement(1838..2437)
                     /locus_tag="PLP58_15610"
     CDS             complement(1838..2437)
                     /locus_tag="PLP58_15610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ10005.1"
                     /translation="MKIRGSLAGDVAHGAFGFALVGVLSFSIWACCAGWFRNRGGEPA
                     MYAAIALAFLALSVLVLAPLAGGAGRFVRAFYPAFLAYAVLWSAAWFAMPNRAGEWTG
                     AALGCLVFAWLLLRLLGRRGGWLAPALVFFALHTVGYFAGSWAMYDYWLGGFKAGQFA
                     DMTRAQAALYAKLSWGLCYGLGFGAGIGLLMHGARRKAA"
     gene            complement(2575..11556)
                     /locus_tag="PLP58_15615"
     CDS             complement(2575..11556)
                     /locus_tag="PLP58_15615"
                     /inference="COORDINATES: protein motif:HMM:NF012200.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choice-of-anchor D domain-containing protein"
                     /protein_id="HRJ10006.1"
                     /translation="MMTPRSLRRLFVWTGLSLTLGAAGLRAQAPLPVWQSSDTVVGDS
                     ATITLAKPAGTAVNDLLFVAVGKSAGAAGSVVTAPGGWSGSVNTGATAQIGFSSFFRV
                     AQAGEPASYDFTITGGVPWVASITRVTGAHRTAPIPHSGALGSLEATGSAMTTGLSTT
                     TANNLMLNYFAARINGTFSTPPSTTERFDAANTTAGHPSMMLATVNMPAAGFAPGHTS
                     TMSASGYYVSAQLAVRPPQLATVVLNSIGYSNYSMLRFSSNISDAGGTDILERGVVYS
                     ETALNSTPTLGGASVLSAVSTDTTNSYDTYAIGFKAATSYTFRAYVTNTVGTAYSTVF
                     PLTTAAPSNLNTFFSGDGNHPVDLGDTDNAANVIVQGDGKPVLLVNRADAGRASLARL
                     TTAGVMDTSFSGDGRADLSVPASTADGLAGVVLQNNGRIVVAGQSTFADGSRRIIVAR
                     FLADGTQDTEFDGDGVAVVDAAGLEYGLTGANAQAYAADVALQTDGKIVVAGRVRRNT
                     GGKDNGLVLRLNADGSVDGTFGGNGVAGVEATSTENTSFAGVEMQPDDKIVLGGSRVV
                     SGFDFQCLAARLTAAGALDSGFGTGGLVLFDANTLEGGGHNEIDQVWDVKLAPDGGIL
                     LAIEAETASFTPRYFVARLNRSNGALDSGFGGNGVINTGVAGSVAKMEVQGDGRILLA
                     GGNAAFNTTVLRLLPTGAFDTDWDSDGILVNTAASSATGVCVGRLGFVFVSALDNAFS
                     GGSAHVFNFRGDTVTLMPTLTPLDNATGAGVNDNLVLSFGTAVVPFTGSIEIIHAHTF
                     AVLESIPVTDARVTGGGTGTITINPDATLAGNTKYYVRVPFHAFRTTPNISPSPGISN
                     TMWEFTTTAPVLSSNADLSALALSAGALDPAFAPGTTAYTATVLNPAASITVTPTKAD
                     AGASIEARVNGGGYAPVTSGSPSGPLALNVGPNTVDVRVTAEDGTTMKTYTVTVTRES
                     APTDITLTPSSRAENNAANATVGTLAAVDGTGPYTFTLAAGDGDADNGSFNILGTALR
                     LTNPVDFETQSSYALRVQVEDDNGYVFAKALTVSITNVNEVPSFTKGANQVHTAASGA
                     QSVPGWATAIDDGDSTVTQAVSFEVTLNSNPGLFTTAPAVAANGTLTYTLSGTTGAAT
                     ITLRILDDTSIGGQPAQASATQNFTITNQPAPNYVVTYAGGHLTCTDISGNGDTLVLR
                     ELTGEAGSVRLFASGRTFSVNGGLNTNSETAAFATADLNSVTLNAGAGNDTITILSNI
                     TGSTLSLPGLTINGGTGDDQVNLSVSITFKPDASLNVDLQDDDATPGTDNILTSSSVQ
                     LLLSGTGGADFKASRRVAFASGSVLEVENGNILVEANQQAVATAGSFSGQEFLNARVE
                     ATGSGSVTLRGRSGNGGAFIMGVAVRDGAQVLAGAGGIVIEGSGQGGHTNGGNYGVSV
                     GAGLTQALVKAVGGDISITGVAGSGGTANLNIGVVVEGQGEVEATGAGKATLHGTGGN
                     TTGTGPNNHGIFLNGSARVTTENGKLTLTGISGGGAGSTTNIGVNLFSALLVGPTGTG
                     DLQITGTPGGAGAGVGVNLTSNALLSTPAGDITISGTAAPQGGDGVSLANTVSANGGD
                     ISITGAGAGTVNGHDGITASQLISTTGTGSVTLHGTAAPAGHGIGVTLTNSTSTVQTG
                     GGGISITGIGGSGGTSNLSAGVRVGGLVSGPAPGGSLTIHGTGGSNGQAFPHGVEFLG
                     ANADAATSGGDISITGIAGDAGAGQAHGVNFVSGAGINADAGSVTLHGTGGAGTGSTV
                     GVQVSATEVVGNGGIHITGVGGTGTGAGINLPSIPSLVTTGGDITLHGTGSTLGGLGI
                     GYSGTIDAGGGDISLTGISTGTVNNLHGIVRSAATTTTGTGTITMHGTASPDGHGSGI
                     LLGGTAPVTTGGGGISLTGIGGGAGTTSGNSGVLVNGAVISGPGAGSALTIHGTGGAS
                     TQNFPHGVEINGGGQLLTSGGDIVVTGIGGANSAAQALGVNIPGGSQISAGGTGAVTL
                     TGTGGAAAGNSQFGVFLNGGTVSTNDGPISVTGTAGDQSGTLSGSRGIYKSSGTVVIV
                     GGDHDLTLVADSMALAGSNTINAAGNDVIIRQKTAGTAITLGAEDTVTGSPLVLGLSD
                     VELDAFVADEVRIGNADTGAMTVAGAISPLSYPVLRLAKPVTFADGGSFVADITSETV
                     FERMIVDGGITLQDTSTITGAVLGGFVPSATAVIPVIQGNDGVTGVFTGRPEGWPMSL
                     TGLTRRMRLTYAAGGGNHIAFYSSPIVVLRGNGQAIAKGDATPSTSDHTDFGGTLTAG
                     SNTVVRTFEILNDGLAPLTGISADVDSGAPAGAFSVTSTPDATLASGVFSIFNVTFDP
                     AANGIQSSTVTVTSDQPLDSPYSFAIQGEGTYPEITVYDGTVLGTELMDDTGEVDFGD
                     VERSTTGAARLFTIRNTGTGPMSGIAVTKGVDGLPAEFGLDVGGLSASLLPNTSTTFT
                     VALTPESLGPRGAMLEIASTDEDENPFEVHLIGEGVPPAFAAVTDQFMLPDQGMDGGG
                     RIYRPLTGVINQDGFITFRAQGAVGSGGITNANDSLLLSDVSGELRVVAREGGVVQTT
                     PWQTLSGLFTHFVLSEGGESVALDRVAGAPTSTDYAYIGSPDGLALEILSREGDAAPS
                     AGVFKTHTAKPAVDAQGRVYFSGDLSGAGITTKTDTGIYQESGGSVVEIIREGDDVSA
                     QTGDPAWLGAVSNYVAAAGDGVVFTASLQNHPTDTTQKTDTALNAVVMAGNEAALAVI
                     ARKGDVVPGTGVATMKSFSGVSRGGADSHAFLGLMNTGGGVTTANDQVLVAAQGGNLH
                     LVAREGVTQVSDVTLTKFADFYACAGGVVIFEASGVVCRWTESGGIEELARAGAAAPG
                     MGGATFSSIGSLSVSDGGAVALVTPLSNGQTALWRALPGGALSLVTQTSDVIVVDGTP
                     QTILGISIHAQGAGTGGGGGGMGAAINDDGYLFATLSLGGGNHVARVFP"
BASE COUNT         1740 a         3923 c         4214 g         1916 t
ORIGIN      
        1 agcgcagcgc taccgctaca aaaactgcga catggccgat ctggaggccc aactcgccgc
       61 cgccgatgcc aaaggcgcgc gctttaaaat gatcgccacc gatggcgtgt tctcgatgga
      121 cggcaccatc gctcccttgc gcgagatttg tgacctcgct gaaaaacatc acgcgctcgt
      181 catggtggac gactcccatg ccaccggttt catcggcagg acaggccgcg gcacgcatga
      241 gcactgcgag gtgatgggcc gcgtggacat tctgaccggc acgctcggca aagccctcgg
      301 cggagccagc ggcggctaca ccagcgggcg caaagaaatc atcgaactgc tgcgccagcg
      361 ctcgcggcct tatctgttct cgaacacgct ctgccccagc atcgccggag cgacgctcga
      421 agcgctgcgt cttctcaaaa ccagcacccg tctgcgcgac acgctggagg cgaacacccg
      481 ctggttccgc gccgccatga cggaggccgg ttttaacatc gctctgggag cccaccccat
      541 cgtccctgtc atgctgggtg acgccgcgct cgccacccgc ttcgccgacg ccatgctgga
      601 gcgcggggtt tatgtcattg gcttcagctt ccccgtcgtc gccgagggca aggcccgcat
      661 ccgcacccag atgagcgccg cccacacgcg cgcggatttg gaaaaggcgg tggcggcatt
      721 tgccgcggtc agagaccagc ttcagcccgg ctgacagcca atcccaacgc tcacccgatg
      781 tgggcggtgt aggcggcggc gtccatgagg gcggcggctt cgtccgggct ggagggcttg
      841 attttaaaga tccagccggc gccatagggg tcggtgttga tgaggccggg ctcggcactg
      901 agcacgccat tcacctcgac gatctcgccg ctgacgggtg tgtagatgtc gctggcggct
      961 ttgacggact caatgacgca gacctgctgg ccggcagtca ccggacccag cttcggcggg
     1021 tccacaaaga ccacatcggt cagctcggcc tgggcgtgat cggtgatgcc tacgggggag
     1081 atttcctgcg cggggtccac ccattcatgg gtgtcgcggt aacggagatg ggcggggact
     1141 tggctcatgg cggtgtggct cttggaaagg ggcggactca atcaaacctg attcaaaccc
     1201 agccgagcgc ggtcatgcgc gagtggatgg catccctggc ctccagcaac tcgccgatgg
     1261 ggtcccagcg gccgctggtg agggcgtcgt gcgcggtggc gggcacggtg acggggatgc
     1321 tctccgcgcc gaagcgcacc tggcggctgg cgacatccac gacgacttcc atccgcgggt
     1381 cagcctccac ggcggcggcg agccgggcga tgtcctccgc cgtggcgcag acgcagggga
     1441 tgccgagcgt ggtgcagttg ccgaagaaaa tctcggcgaa actctgcgcg atgacggccc
     1501 ggaatccgaa gcggtaaagg gcctgcggcg cgtgctcgcg ggagctgccg cagccgaaat
     1561 tgctgcctga gacgaggatg gaggcgccct tgaaacgcgg atcgtccagg ggatgcccgg
     1621 cgggcttgcc atccttgtcg aagcgcacgt cgtggaaggc gaactcgccc agcccgtcga
     1681 aggtgacgca tttcatgaag cgtgcgggga tgatgcggtc cgtgtcaatg tcggagcctg
     1741 ggacgtgaac ggcggtgccg gtgacttggg tgatctttgc gagggccatg agggcgcgag
     1801 aataggcagg gaatccgcgc ggggcaaatg ccagatttca tgccgccttc cgccgcgccc
     1861 cgtgcatgag caggccgatg cccgcgccga agccgaggcc gtagcacagc ccccaggaga
     1921 gcttggcata gagagccgcc tgcgcgcgtg tcatgtccgc gaactgcccg gccttgaacc
     1981 cgcccagcca gtagtcatac atggcccaac tgcccgcgaa gtaacccacc gtgtgcagcg
     2041 cgaagaacac cagcgccggc gccagccagc cgccccggcg gccgagcagg cgcagcaaga
     2101 gccaggcaaa caccaggcag cccagggccg cgcctgtcca ctcccccgcg cggttcggca
     2161 tggcgaacca ggccgcgctc cacaaaaccg cgtaggccag gaaagccgga taaaaagcgc
     2221 gcacgaacct gcccgcgcca cccgccagcg gcgcgagcac cagcacggac agcgccagga
     2281 acgcaagcgc gatggcggca tacatggccg gctcccctcc cctgttccgg aaccacccgg
     2341 cgcagcaggc ccatatcgaa aaggacagca cacccaccag cgcaaagcca aaagcgccat
     2401 gcgccacgtc accagccagg gaaccacgaa ttttcatgcg acactgaacg cggcatccaa
     2461 gaccaggctt ccaggggatt tgcaccagtg gcacagacac acctgcctgc ctggactggc
     2521 gcgttcctca atgaggcttg tggcgggaag cgcgtccgcg tggagcagcc cgcctcacgg
     2581 gaagacccgc gccacatggt tgccgccgcc gagggagagg gtggcgaaga ggtagccgtc
     2641 gtcgttgatg gccgcgccca tgccgccgcc tccgccgccg gtgcccgcgc cctgcgcgtg
     2701 gatggagatg cccaggatgg tctgcggcgt tccatccacc acgatgacat cgctggtctg
     2761 cgtcaccagg ctcagcgcgc cgccgggcag cgcgcgccac agggcggtct ggccgttgga
     2821 gagcggtgtc accagcgcca ccgcgccgcc gtcgctcacc gacaggctgc cgatgctgct
     2881 gaaggtggcc ccgcccatgc cgggggccgc cgcgccagcg cgcgccagct cctcaatgcc
     2941 gccggattcc gtccagcggc acaccacgcc gctggcctcg aagatcacca ccccgccggc
     3001 gcaggcgtaa aagtcggcga acttcgtcaa agtcacgtcc gagacctgcg tcacgccctc
     3061 gcgcgccacg agatgcaggt tgccgccttg cgcggccacg agcacctggt cgttggctgt
     3121 ggtcacgccg ccgccggtgt tcatgaggcc gaggaaggcg tggctgtccg cgccgccgcg
     3181 ggagacgccg gagaaggact tcatggtggc cacgccggtg ccgggcacca catcaccttt
     3241 gcgggcgatg acggccaggg cggcttcatt gcccgccatg accacggcgt tgagcgcggt
     3301 gtcggttttc tgggtggtgt ccgtcgggtg gttctggagg ctggcggtga agaccacgcc
     3361 gtcaccggcg gcggccacat agttcgacac cgcgccgagc caggcggggt cgccggtctg
     3421 cgcgctcacg tcatcgccct cgcggatgat ctccaccaca ctgccgccgc tttcctggta
     3481 gatgccggtg tcggtcttgg tggtgatgcc cgcgccgctg aggtcgccgg agaaatacac
     3541 cctgccctgc gcgtccaccg ccggtttggc cgtgtgggtc ttgaacacac ccgcgcttgg
     3601 cgcggcgtcc ccttcgcggc tgaggatctc cagcgccaga ccgtcgggcg agccgatgta
     3661 ggcgtagtcg gtgctggttg gcgcgccggc gacgcggtcc agagccacgc tctcgccgcc
     3721 ctcgctgagc acgaagtggg tgaacaggcc cgacagcgtc tgccagggcg tggtctgcac
     3781 cacgccgccc tcgcgcgcca ccacgcgcag ctcgccggag acatcgctga gcagcaggct
     3841 gtcgttggca ttggtgatgc cgccgctgcc caccgcaccc tgcgcgcgga aggtgatgaa
     3901 gccgtcctgg ttgatcaccc ccgtcagcgg ccggtagatg cgcccgccgc cgtccatacc
     3961 ctggtcgggc agcatgaact ggtccgtcac cgccgcgaac gcgggcggca cgccctcgcc
     4021 gatgaggtgg acctcgaagg ggttctcgtc ctcgtcggtg ctggcgatct ccagcatggc
     4081 gccgcgcggg ccgagcgatt caggcgtcag cgccacggtg aaggtggtgc tggtgttcgg
     4141 cagcaggctg gcgctcaggc cgcccacatc caggccgaac tccgccggca gcccgtccac
     4201 gcccttggtc accgcaatgc cgctcatggg gccggtgccg gtgttgcgga tggtgaagag
     4261 ccgcgccgcc ccggtggtgg agcgctccac atcgccaaag tccacctcgc ccgtgtcatc
     4321 catcagctcc gtgcccagga cggtgccgtc atacaccgtg atctcgggat acgtcccctc
     4381 cccctggatg gcgaagctgt aggggctgtc gagcggctgg tcgctcgtca ccgtcaccgt
     4441 gctggactgg atgccgttgg ccgccgggtc aaaggtgaca ttgaagatgc tgaacacccc
     4501 ggaggccagg gtggcgtccg gtgtgctggt gacgctgaaa gccccggcag gcgcaccgct
     4561 gtccacatcg gcggagatgc cggtcagcgg agccaggccg tcattgagga tctcgaaggt
     4621 gcgcaccacc gtgttgctgc ccgcggtcag cgtgccgccg aagtccgtgt ggtcagaggt
     4681 ggaaggcgtg gcgtcaccct tggcgatggc ctggccgttg ccgcgcagca ccacgatggg
     4741 cgagctgtag aaggcgatgt ggttgccgcc gcccgccgcg taggtcaggc gcatgcgccg
     4801 cgtcaggccg gtgaggctca tgggccagcc ttccggccgg ccggtgaaga cgccggtcac
     4861 gccgtcgttg ccctggatga ccgggatcac cgccgtggcg gagggcacga agccgcccag
     4921 caccgcgccg gtgatcgtgg aggtgtcctg gagggtgatg ccgccgtcca cgatcatgcg
     4981 ctcgaagacc gtctccgagg tgatgtcagc cacaaagctg ccgccatccg caaaggtgac
     5041 gggcttggcc agccgcagca ccgggtagct cagcggggaa atggcgcccg ccacggtcat
     5101 cgcgccggtg tcggcattgc cgatgcgcac ctcgtcagca acgaaggcat ccagctccac
     5161 atccgacaag ccgagcacca gcgggctgcc ggtgacggtg tcctccgcgc ccagcgtgat
     5221 ggcggtaccg gcggtcttct ggcggatgat cacgtcattg cccgccgcgt tgatcgtgtt
     5281 gctgcccgcc aatgccatgc tgtcggccac cagcgtcagg tcgtggtcgc cgccgacgat
     5341 gacgacggtc ccgctggact tgtagatgcc gcggctcccg ctcagggtgc cgctttggtc
     5401 gcctgccgtg ccggtgacgg agatggggcc gtcgttggtg gacaccgtgc cgccgttgag
     5461 gaagaccccg aactgcgaat tgcccgccgc cgcgccgccg gtgccggtga gggtgaccgc
     5521 gcccgtgccg cccgcgctga tttgagagcc gccggggata ttgacgccca gggcttgcgc
     5581 ggcactgttc gccccgccaa tgccggtgac aacaatgtca ccgccgctgg tgagcaactg
     5641 cccgccgccg ttgatctcaa cgccgtgcgg gaagttctgc gtgctggcgc cgcccgtgcc
     5701 gtggattgtc agcgcggaac ccgcgccggg accgctgatg acggctccgt tcaccaggac
     5761 acccgagttg cctgaggtgg tcccggcgcc gccgccgatg ccggtcaggg agatgccgcc
     5821 accgccagtc gtcaccgggg ctgtgccgcc aagcaaaatc cccgagccat gcccgtccgg
     5881 gctggcggtg ccgtgcatgg tgatggtgcc cgtgccggtg gtggtggtgg ccgcactgcg
     5941 cacgatgccg tgcaggttgt tcaccgtgcc tgtgctgatg ccggtgaggc tgatgtcacc
     6001 cccgcccgcg tcaatcgtgc cagaatagcc gatgcccagg ccgcccaggg tgctgccggt
     6061 gccatgcagg gtgatgtccc cgccggtcgt gaccagggat gggatggaag gcaggttgat
     6121 gcccgcgccc gtgcccgtgc cgcccacgcc ggtgatgtgg atgccgccgt tgccgaccac
     6181 ctcggtggcg ctgacctgca cgccaacggt ggagcccgtg cccgcgccgc cggtgccgtg
     6241 gagggtgacg ctgccggcgt ccgcgttgat cccggctcca ctgacaaagt tcacgccgtg
     6301 ggcctgcccg gcgcccgcgt ccccggcgat gccggtgatg ctgatgtcgc cgccggaggt
     6361 ggcggcgtcg gcgttggcgc cgaggaactc caccccgtgc ggaaaggcct ggccgttgct
     6421 gccgccggtg ccgtggatgg tgaggctgcc gcctggggcg gggccggaga ccaggccgcc
     6481 cacgcgcacg cccgcgctca gattgctcgt gccgccgctg ccgccgatgc cggtgatgct
     6541 gatgcctccc ccgccggtct gcacggtgct ggtgctgttg gtcagggtga caccgatgcc
     6601 gtgccccgcc ggggcggcgg tgccgtgcag ggtgacgctg ccggtgccgg tggtgctgat
     6661 gagctggctg gccgtgatgc cgtcgtgacc attcaccgtg ccggcgcccg cgccggtgat
     6721 gctgatgtca ccgccgtttg cgctcaccgt gttcgcgagg ctcaccccgt ccccgccctg
     6781 aggcgccgcc gtgccggaga tggtgatgtc acccgccggg gtgctgagca gggcgttgct
     6841 ggtgagattc acaccaacgc ccgcgcccgc gccgcccggc gtgccggtga tctgcaggtc
     6901 ccccgtgccg gtggggccga cgaggagggc gctgaacagg ttcacgccaa tattggtggt
     6961 gctgcccgcg ccgccgccgc tgatgccggt cagggtcagc ttgccgtttt ccgtcgtcac
     7021 ccgggcggag ccgttgagga agatgccgtg gttgttcggc cccgtgcctg tggtgttgcc
     7081 gcccgtgccg tgcagggtcg ccttgcccgc gccggtggcc tccacctcgc cctgcccctc
     7141 caccaccaca ccgatgttca ggttggcggt gccgccggag ccggccacgc cggtgatgct
     7201 gatgtccccg ccgacggctt tcaccaaggc ctgggtgagg ccggcgccca cgctcacgcc
     7261 gtagttgccg ccgttggtgt gcccgccctg gccgctgcct tcgatgacga tgccgcccgc
     7321 gcccgccagc acctgggcgc cgtcgcgcac ggccacgccc atgatgaagg cgccgccatt
     7381 gccggagcgc ccgcgcaggg tcacgctgcc ggaaccggtg gcctccaccc gggcgttcaa
     7441 gaactcctgc cccgaaaagc tccccgccgt ggccacggcc tgctggttgg cctccacgag
     7501 gatgttgccg ttctccacct ccagcaccga gccgctggcg aaagccaccc gccggctggc
     7561 tttgaagtcc gccccgcccg tgccggagag caggagctgc acgctgctgg aggtgaggat
     7621 gttgtccgtg ccgggggtgg cgtcgtcatc ctgcaggtcc acgttcaggc tggcgtcggg
     7681 cttgaaggtg atggagacac tcaagttcac ctggtcatcc cccgtgccgc cgttgatcgt
     7741 caggccgggc aggctcaggg tgctgcccgt gatgttggag agtatggtga tcgtgtcatt
     7801 gcccgcgccg gcgttcaggg tcacgctgtt gaggtccgcc gtggcgaagg ccgcggtctc
     7861 ggagttggtg ttcaggccgc cgttgacgct gaaggtgcgc ccggaggcga agaggcgcac
     7921 gctgcccgcc tcgcctgtca gctcacggag aacgagggtg tccccgttgc cgctgatgtc
     7981 cgtgcaggtg aggtgcccgc cggcgtaggt caccacatag tttggcgcgg gctggttggt
     8041 gatggtgaag ttctgcgtgg cgctggcctg cgcgggctgg ccgccgatgc tggtgtcgtc
     8101 caggatgcgc agggtgatgg tggccgcgcc tgtcgtgccg ctgagggtgt aggtcagcgt
     8161 gccgttggca gccacggcgg gggcggtggt gaagaggccg gggttgctgt tgagggtcac
     8221 ctcgaagctc accgcctgcg tcacggtgct gtccccgtcg tcaatggccg tggcccagcc
     8281 gggcacgctc tgcgcgccgc tggcggcggt gtggacctgg ttggcgccct tggtgaagct
     8341 ggggacttcg ttgacgttgg tgatgctcac cgtcagggcc ttggcaaaga catagccatt
     8401 gtcgtcctcc acctgcacgc gcagggcgta gctgctctgg gtctcgaagt cgacggggtt
     8461 ggtcagccgc agggcggtgc cgaggatgtt gaagctgccg ttgtcagcgt ccccgtcccc
     8521 ggcggcgagg gtgaaggtgt aggggcccgt gccatccacc gccgccaggg tgccgacggt
     8581 ggcgttggcg gcgttgttct cggcgcggct tgagggcgtc agcgtgatgt ccgtgggggc
     8641 ggactcgcgg gtgacggtga cggtgtaggt cttcatcgtc gtgccgtcct cggcggtgac
     8701 gcgcacatcc acggtgttgg ggccgacgtt gagggccagg gggccgctgg ggctgccgga
     8761 ggtgacgggg gcgtagccgc cgccattgac gcgcgcctcg atgctggcgc cggcgtcggc
     8821 cttggtgggg gtgacggtga tgctggcggc ggggttgagc acggtggcgg tgtaggcggt
     8881 ggtgccgggg gcgaaggcgg ggtccagcgc gccggcgctc agggccaggg cggagaggtc
     8941 ggcattgctg ctgagcacgg gggcggtggt ggtgaactcc cacatcgtgt tggagatgcc
     9001 gggggaggga ctgatgttgg gggtggtcct gaaggcgtga aagggcaccc gcacataata
     9061 cttggtgttg cccgccaggg tggcgtccgg gttgatggtg atggtgccgg tgccgccgcc
     9121 ggtgacgcgc gcgtccgtga cggggatgct ctccagcacc gcaaaggtgt gcgcgtggat
     9181 gatctcgatg ctcccggtga acggcacgac cgcagtgcca aaggacagga ccaggttgtc
     9241 attgacgccc gcgccggtgg cattgtccaa gggtgtgagg gtgggcatga gtgtcacggt
     9301 gtctccccgg aagttgaaga cgtgggcgct gccgccgctg aaggcgttgt ccagcgcgct
     9361 gacaaagacg aacccgagcc tgccgacgca gacgcccgtg gcggaggagg ccgcagtgtt
     9421 gacgaggatg ccgtcgctgt cccagtccgt gtcaaaggcg ccggtgggca gcaggcgcag
     9481 cacggtggtg ttgaaggcgg cattgccccc ggccaggagg atgcgcccgt ccccctgcac
     9541 ctccattttg gcgacgctgc cggccacgcc ggtgttgatg acgccgttgc cgccgaagcc
     9601 gctgtccagc gcgccgttgc tgcggttgag ccgggccacg aagtagcgcg gggtgaagga
     9661 ggccgtctcc gcctcgatgg ccaggaggat gccgccgtcc ggggccagct tcacatccca
     9721 gacctggtct atctcattgt ggccgccgcc ctccagggtg ttggcgtcga aaagcaccag
     9781 cccgcccgtg ccgaagccgc tgtccagcgc gcccgccgcc gtcagccgcg ccgccaggca
     9841 ctggaagtcg aagccgctga ccacccgcga gccgcccagg acgatcttgt catccggctg
     9901 catctccacg ccggcgaagc tggtgttctc cgtgctggtg gcctccacgc cggccacgcc
     9961 gttgccgccg aacgtgccgt ccacgctgcc atccgcgttg agccgcagca ccaggccgtt
    10021 gtccttgccg ccggtgttgc gccgcacgcg gcccgccacc acgatcttgc cgtcggtctg
    10081 caaggccacg tcggcggcgt aggcctgcgc gttggcgccg gtgaggccgt actccaggcc
    10141 cgccgcgtcc accaccgcca cgccgtcgcc atcaaactcg gtgtcctgcg tgccatcggc
    10201 caaaaagcgc gccacgatga tgcggcggct gccgtcggcg aaggtggact gccccgccac
    10261 cacgatcctg ccattgttct ggagcaccac gcccgccagc ccgtccgccg tggaggccgg
    10321 cacgctgagg tccgcgcgcc cgtccccgct gaagctggtg tccatgacgc ccgcggtagt
    10381 gagccgcgcc agggaggcgc gcccggcgtc ggcgcggttg accagcagca ccggcttgcc
    10441 atccccctgg acgatgacat tggcggcgtt gtccgtgtcc cccaggtcca cggggtggtt
    10501 gccgtccccg ctgaagaacg tgttcaggtt cgagggcgcg gcagtggtca gggggaaaac
    10561 cgtactgtaa gcggtgccca ccgtattggt cacataggcg cggaaggtgt agctggtggc
    10621 ggccttgaac ccgatcgcgt aggtgtcgta gctgttcgtg gtgtccgtgg agactgcgga
    10681 gagcacgctg gccccgccca gggtgggcgt gctgttgagc gcggtctcgg agtaaacgac
    10741 gccgcgctcc aggatgtcgg tcccgcccgc gtcgctgatg ttgctggaga accgcagcat
    10801 ggaataattg ctgtagccga tgctgttgag gaccacggtg gccagttgcg gcgggcgcac
    10861 ggcgagctgc gcgctcacat agtagccgct ggcgctcatg gtggaggtgt ggccgggggc
    10921 aaagcccgcc gcgggcatgt tgacggtggc cagcatcatg gaggggtggc ccgcagtggt
    10981 gttggccgcg tcgaaccgct ccgtggtgga ggggggcgtg ctgaaggtgc cgttgatccg
    11041 cgccgcgaag tagttgagca tcaggttgtt ggccgtcgtc gtggacaggc ctgttgtcat
    11101 cgccgagccg gtggcctcca ggctgcccag agccccggaa tgcgggatgg gcgcggtgcg
    11161 gtgcgcgccg gtgacgcggg tgatggaggc cacccagggc acgccgccag tgatggtgaa
    11221 gtcatagctg gccggctccc ccgcctgcgc cacgcggaaa aaggagctga agccgatctg
    11281 cgcggtggcg ccggtgttga cgctcccgct ccagccaccg ggcgcggtga ccacggagcc
    11341 ggccgcgccc gcgctcttgc ccacggcgac aaacagcagg tcattgaccg ccgtgcccgc
    11401 gggcttggcc agggtgatgg tggcgctgtc acccaccacg gtgtcactgg actgccacac
    11461 aggcaggggc gcctgggcgc gcagcccggc cgcgccgagg gtgagtgaga gacctgtcca
    11521 gacgaaaaga cggcggaggg agcggggagt catcataggt taggcgggtt gtgcagtccg
    11581 atttcaggaa aaaggccgca tttcagtcat ttaagggcat taccgccaaa atgtgacctc
    11641 aaaaacacaa aataagtgtc agacagggtg cgggagtcgc agatttgaga aagaccctcc
    11701 tatctccgcc ccgaacccgc ccctagccgt ttccccggaa gggtgcgccg ccgggatgcc
    11761 gtaggccggg tgtggcgcag cccgcccggc ttt
//
LOCUS       DAOMYJ010000165        11715 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Prosthecobacter sp. isolate AMR_MDS_5629
            NODE_1825_length_11715_cov_19.330961, whole genome shotgun
            sequence.
ACCESSION   DAOMYJ010000165 DAOMYJ010000000
VERSION     DAOMYJ010000165.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867850
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prosthecobacter sp. (wastewater metagenome)
  ORGANISM  Prosthecobacter sp.
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Verrucomicrobiaceae; Prosthecobacter.
REFERENCE   1  (bases 1 to 11715)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11715)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.30
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:34:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,742
            CDSs (total)                      :: 4,684
            Genes (coding)                    :: 4,676
            CDSs (with protein)               :: 4,676
            Genes (RNA)                       :: 58
            tRNAs                             :: 55
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11715
                     /organism="Prosthecobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1825_length_11715_cov_19.330961"
                     /isolate="AMR_MDS_5629"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1965333"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(900..1319)
                     /locus_tag="PLP58_15620"
     CDS             complement(900..1319)
                     /locus_tag="PLP58_15620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ10007.1"
                     /translation="MAAKNSNSRKKKVCHSSSASRLQNSGTGSLDSAQRLPHGQPSRH
                     LPAFSAGISWVSGFRQQTPTCFGLMNKQAEESRYFTANIRCSRCLDLLDQKTFMKNEA
                     NPEKALTVKAWMTKLREKNMPKYRPRAISHHTNYTVR"
     gene            complement(1506..2894)
                     /locus_tag="PLP58_15625"
     CDS             complement(1506..2894)
                     /locus_tag="PLP58_15625"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004326 - tetrahydrofolylpolyglutamate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008841 - dihydrofolate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="folylpolyglutamate synthase/dihydrofolate
                     synthase family protein"
                     /protein_id="HRJ10008.1"
                     /translation="MRTQSGGGDKIFVRLCGAEVSERVFGGGADLSWQAGAGAACCGA
                     HTSMMNDSSPAPASLTPNLDWLYGTQLYGMKPGLDNTRRLLEETGLPGAGVRFLHVAG
                     TNGKGSTCAFMHSMLGAAGVRAGLFTSPHLVRFNERIRDTEREITDAEIESGLARIRA
                     LVADWQPHPTFFEITLALALDWFQARGLEWVVLETGLGGRLDATNVVTPEVAVITRIG
                     MDHMAQLGGTLAEIAAEKAGIIKPGVPVVTGPQEPEALRVIKSAARSKKAPLIVVDAP
                     LNEVPLGLSGPHQGWNAALAVAALRETGLPLPALVMEQGLRETVWPARFQSFGQGRVI
                     LDGAHNPDAARALAEAWRAAFPGENAVVVFGGSSGKDHAETLLPLMEISARWVLTGFD
                     SPRSVPLPVLEEAFLAAGGEARLCEQARTLPEALALASRHGARVLICGSLFLAGEYLA
                     LRAQERHQPSEQ"
     gene            2901..3380
                     /locus_tag="PLP58_15630"
     CDS             2901..3380
                     /locus_tag="PLP58_15630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ10009.1"
                     /translation="MNDLERRVMAAQGYLELGLHAEAAEEMRHLPKEAFERGDVIEIS
                     LLCLMEQKRWGDALVVAEKLCRLEPRQAGGFIHAAFCLHELGRTREALDILARGPASL
                     KTKPVYYYNLGCYLACLGEEEKAVALLLQSFEMDGTLRGHARHDPDLDRLRPRLETA"
     gene            3396..4565
                     /locus_tag="PLP58_15635"
     CDS             3396..4565
                     /locus_tag="PLP58_15635"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HRJ10010.1"
                     /translation="MAAVSQSALHAATRWFDENFQTRGELGASVSVWQNGVEILSLSH
                     GVRDRQGARPWERDTLVPVFSATKAPAAVCCLLALHEAGFPLDCPVAEAWPEFVGGGK
                     SRMHFAHLLSHTSGLCALDEKAPIYNYDAVIQALEMQPPLWEPGTRQGYHARTFGFLM
                     DEIVRRVTGADSLGQFFRDTFGDPMNLDFWIGLPEQQWPRVSPVHPGKISIASGDQPF
                     LKAWNTSGTLTQRAFSSPFGLSSISEYNQSAFWSPGYASMGGVGSARGLGKFYAMLAQ
                     GGSWGGARLVPAAVLAQLQHTLSQEEDAVLLTPAAFAAGVMQDPLDPDPESGGGKLRR
                     HFGPSLSAFGHPGAGGSLAFADPENGLAFAYVMNQMEAGPMPGAKVLGIVERLYA"
     gene            4576..5556
                     /locus_tag="PLP58_15640"
     CDS             4576..5556
                     /locus_tag="PLP58_15640"
                     /inference="COORDINATES: protein motif:HMM:NF012607.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C-terminal binding protein"
                     /protein_id="HRJ10011.1"
                     /translation="MSLPHIAVIDWRASPQGDDDCSIERSVIGSTARLTRHLCDTDAD
                     LDDEAAGADAIILWHNMPLTARGISRLVKCRAIIRNGVGYDSVDTTAARARGIPVCNV
                     PDYGTEEVADHAIALALALCRQLFPLDAEAKALGWEIKVRGKLRRLRGLNFGIVGLGR
                     IGTATALRAKALGFRVVFYDPCLPNGADKALGIERAWSLDELLMQAGVLSLHCPLNDT
                     TRHLIGARELALLPESAHVVNTARGGLIDKTALLAALREGRIAGAGLDVIEDEPLRTP
                     EEAATPNLIVTCHAAYYSGMSVVEMSRTSARIALAAVTGAPLFNVVNGVV"
     gene            complement(5880..7301)
                     /locus_tag="PLP58_15645"
     CDS             complement(5880..7301)
                     /locus_tag="PLP58_15645"
                     /EC_number="4.1.99.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009963383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003904 - deoxyribodipyrimidine
                     photo-lyase activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribodipyrimidine photo-lyase"
                     /protein_id="HRJ10012.1"
                     /translation="MPAHQVVIHWFRRDLRLTDNPALLHAARSAAGVIPLYILSDWKK
                     HHDWTGPARQHFLCGSLASLAKNLEAIGSRLSLRSGTAEAVLEKLIHETGASAVFFNR
                     DYDPFGRAAESGVRALCARLGVACHDHKDRVLHEPEEALTGAGGPYRVYTPYSRAWLA
                     LEKPRPEGRVKALGPAPALPSLPLPSLAWWQLPECTAAIPEPGEKAARQRMKTFIESR
                     VLHDYKPRRDVPEGVTTSRLGQDLRFGLISIRELHSRCQEAMAADSSPQARLSIETYI
                     KELVWREFYLAILWHYPGVFEEEFSPDFRGLHWPGTDAHFQAWCQGRTGFPIVDAGIR
                     QLLATGFMHNRLRMITAMFLTKDLHCDWRLGESFFMRHLVDGENASNNGGWQWSAGTG
                     ADAAPYFRIQNPWTQSKRHDPQARYIQTWVPELRGLPADLLLDPPKDGRPVAPGYPAP
                     IVDHAAERDRTLALFARHKEQRR"
     gene            complement(7398..11354)
                     /locus_tag="PLP58_15650"
     CDS             complement(7398..11354)
                     /locus_tag="PLP58_15650"
                     /inference="COORDINATES: protein motif:HMM:NF012311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HRJ10013.1"
                     /translation="MVPVLLILALAAAWWLREEHGPVGAEGALSMERKTAAMLAEENW
                     EHRALADLYAGRPVLLMENGRPLRMELLLDRLYSRALPVAQRWIPVHAADASTLLREA
                     ARLGDQSGLVLRLARVSHAPKENPALPRAGDDSAGLVVLTGKWLLQYRDRAVALAALD
                     AAGFDLVSEPDYARGQLIVTARAGGAEGGLKAAAALHGHTAIVSASPLLLRQKHKHAD
                     PNDPLFFRQWHLKAAAASASKPGADIQVLPVWSAGGTGQGVRIAIVDDGLDMLHPDLL
                     PNLDSQDNHWDWNDTPQDNDPTGNFLNEDFHGTAVAGLAAARGGNSLGVSGVAPQATL
                     VGFRLISLPTDDSDDAEAMLRGNDVIQIKNNSWGAAPTILHPEFFPELGWSGPLTVAA
                     RRTAALEGRGGLGTLSVWAAGNDRDRGDQGNKDSYPNSIYGIAVGVLTQTGALANYSE
                     GGSHLTVVAPGATGVVTTDYRGAQGYNTGTKPGELTGSPEMRDYTQTFGGTSASAPVV
                     AGVLALMLEVNPQLGWRDVKEILLRSSTHIFPTQAGWVTRAGGAPPSMPPIKHHELYG
                     GGAVSAQAAVAMAETWENLGPMIEDARDLEPNVIIPDNNAAGVTRTFDFSNVTDLRVE
                     HVTVTLDIAHTFRGDLHITLTSPAGTVSTLAAKTSQDDGLDYDGWTFSSVRHWGESAS
                     GLWTLRCQDLARGDTGTLHSAEVRLYGSQVQPAALTLQPVPVIVPEGETAAFTAAGEG
                     GGHVTFQWRKTGAATVLGDGSASFAINSAKLADAGGYTVTATNATGTETSPEAALGVL
                     RRTVAGQSVAEGKTAVLKTAAAGPGLSLRWFRDGTPLNDDGRVTGSGSATLTIKNTAA
                     GDIGAYTCRASFDPPGTVELHTLPAQLDVRLKPVVQPPAVVPAIVSGAAALQFTAAHG
                     ATRFSIKKTPKGMKFSTTTGMLSGTPDLPDTHEIIITAFNASGASQPLTFLWQVEPLP
                     DGTPGAYHGLVDGGTELTGGLGGRFTATLTPTGAFSGNLTLGAESLRFSDRLKALPSG
                     AAPPEATVIIKRAGGRPPVTLAFEVQDGIITGSLDDGEKPPAGFTARRHDWNPALQTV
                     LSGEHTAYFKPDPAFAEDPASPQGSGHIAGTLNAKGLIAWTGRLGDGQTLTCSTPHLL
                     GGRLAFHALVAKKSGSAQGWLILDDTNAGLSGTPEHRRAGLPTLMLGAHGGRYTPVSH
                     LFNFLSAPAGDGNAEAVFAENDVTELFRQPLTLLAPSTLRVFGDNPNRLSLQVNARRG
                     TFTGSFTLPGSPARRAAITGAFVRLPGADPGQGHGYYLLPGPDGLISGGAHLIVPD"
BASE COUNT         1956 a         3651 c         3996 g         2112 t
ORIGIN      
        1 agacgttgct gacacgacag acagccttcg agccgttgcc tgcatccgtt ttgttcggcc
       61 tagtgttcac ggtgaggttg tcgatgtggg agtctgaact gcaatggctg cgacaagctg
      121 ccatccaatg aaaaggacca accagagcag cagcagcaaa ggcatcgcac ccaaaacctt
      181 acccaaataa gaagtgcctt cgccgactgt cgcgatcaaa agccatacat ctgtcacgac
      241 tcccaccagc agagcgcaca cagccaatgt cctcccaagt gccaggcctc gcatggccca
      301 tgcaacaccg gccagcgatg cgcctacgat gctcaaagac gagatgactc ctgatcccca
      361 accatgaccg gcgcctgtaa agaggacact aagaccgccg atcaccaatc caacgacaag
      421 tcccagcgcc agggaaccca agacactgcc tgcgctgatt ctgttagcgt cactcattta
      481 ggttgggcga acgtctagct catccacggc ggggatggaa gtccgaattc aaactggagc
      541 gttacccgcc gttggatgga gcggcttgtt cgctgtcttg gattcatggt cgcttcggga
      601 tggactgctc aaatcgagtt ctgaagtagg gcattctatt gggtaaggac atagactccg
      661 cagcacgttc aagatcacgc cagaatcccc ctcgacacgt aaccagctca ccaagctcct
      721 gcaacatatc cagagcaagt tctagttcat tgtggtcgag aaacgaactg aactctgatt
      781 gaagtcgctc aaactcggca gcgacttccg tcggtagctc aggtaaagcg cggtgagccg
      841 atctcaaatg ggcctcggta accttccatt gcttccatag ttccggcgta gtcatttctt
      901 cagcgaacag tgtaattcgt gtgatgtgat atcgctcggg ggcgatattt tggcatgttt
      961 ttctcgcgga gtttggtcat ccaagccttg acagtcaatg ctttttctgg attggcttca
     1021 ttcttcatga atgttttttg atccagcaaa tccaggcatc tggagcaacg gatatttgcg
     1081 gtgaaatatc gtgattcctc ggcttgcttg ttcattaacc cgaagcatgt gggagtttgt
     1141 tgccggaaac ccgaaaccca agagatgccc gccgaaaagg cggggaggtg gcgggagggt
     1201 tggccgtggg ggaggcgctg ggctgagtcg agactgcctg tgccgctgtt ttgaagccgg
     1261 gaagccgagg aagagtggca gactttcttt ttgcggctat ttgagttttt tgcggccatt
     1321 cattcccgga ttccggctca ggcgctcatt tcattggctc cgcccagcac cctgaaacgc
     1381 gggtcgcggt gcctggacat tggcactcgt gccccgctga cgcggcggga gtgtgagggc
     1441 aatcgaaatt cgaagctcga agctcgaagc tcaattttcg attttcgatt ttcgattttt
     1501 tccccttact gctcgctggg ctggtgcctc tcctgcgcgc gcagggccag gtattccccc
     1561 gcgaggaaga gggagccgca gatgagcacc cgcgctccat ggcgcgatgc gagggccagc
     1621 gcctcgggca aggtccgcgc ctgctcgcac aatcgcgcct cgccgccggc ggcgaggaag
     1681 gcttcttcca gcacgggcaa aggcacgctg cgcggggaat cgaagcccgt gagcacccag
     1741 cgcgcggaga tttccatgag cggcagcagc gtctccgcgt ggtccttgcc gctcgatccg
     1801 ccaaagacca ccaccgcatt ctctccggga aaagccgcgc gccatgcctc cgccaatgcc
     1861 cgcgccgcgt ccgggttgtg cgcgccatcc aggatgacgc ggccttggcc aaagctctga
     1921 aaccgcgccg gccacaccgt ctcccgcagt ccctgctcca tcaccagcgc gggcagcggc
     1981 aggcccgtct cgcgcagggc ggcgaccgcc agcgcggcgt tccagccttg atgaggtcct
     2041 gacagaccga gcgggacttc atttaatggc gcgtccacca cgatgagcgg cgcttttttc
     2101 gaccgcgccg ccgacttgat gacgcgcagc gcctccggtt cctgcgggcc cgtcaccacc
     2161 ggcacaccag gcttgatgat gcccgccttt tccgccgcga tctccgccag cgtgccgccg
     2221 agctgcgcca tgtggtccat gccgatgcgt gtgatcaccg ccacttccgg cgtgaccacg
     2281 tttgtggcgt cgaggcggcc gcccagcccc gtctccagga ccacccactc cagcccgcgc
     2341 gcctggaacc agtccagggc aagcgccagc gtgatctcga aaaaagtcgg gtgcggctgc
     2401 cagtcagcga cgagcgcacg gatgcgcgcc aggccggact cgatctccgc atccgtgatc
     2461 tcgcgctccg tgtcgcggat gcgctcgttg aagcgcacca gatgcggcga ggtgaacaag
     2521 cccgcgcgga cgcccgccgc acccagcatg gagtgcatga aggcgcaggt ggagcctttg
     2581 ccgttggtgc ccgccacatg caggaatctc acgcccgcgc ccggcagccc ggtttcctcc
     2641 agcagccgcc gtgtgttgtc cagccccggt ttcatgccgt aaagctgcgt gccgtagagc
     2701 cagtccagat tcggcgtcag ggaggccggg gcgggggagc tgtcgttcat catgcttgtg
     2761 tgcgcgccgc agcatgccgc gcccgcgcca gcctgccatg acaaatccgc gccgcctcca
     2821 aaaacacgct ccgaaacttc cgccccacaa agcctgacaa aaatcttgtc gccgccgccg
     2881 ctttgcgttc tcatcgcgcg atgaacgatc tggaaaggcg cgtcatggcc gcccagggct
     2941 acctcgaact cggtctccac gcggaggccg cggaggagat gcgccatctg ccgaaggaag
     3001 ccttcgagcg cggcgatgtc attgagatca gcctgctctg cctcatggag caaaagcgct
     3061 ggggggacgc ccttgtcgtc gccgaaaagc tctgccggct cgaaccgcgc caggccggcg
     3121 gattcatcca tgccgccttc tgcctgcacg agctgggccg cacgcgcgag gcgctcgaca
     3181 tcctcgcccg cggccccgcc agcctcaaga ccaagcccgt ctattattac aatctaggct
     3241 gctacctcgc ctgcctcggc gaggaggaaa aagctgtcgc cctcctgctc cagtccttcg
     3301 agatggatgg caccctgcgc ggccatgccc gccatgatcc cgacctggac cgtctgcgtc
     3361 cacggctgga gaccgcctga ttatccgctt ccctcatggc cgccgtctca cagtccgcat
     3421 tgcacgccgc cacacgctgg tttgatgaga atttccaaac ccgcggcgaa ctcggcgcct
     3481 ccgtctccgt ctggcaaaac ggcgtcgaaa tcctctccct ctctcatggc gtgcgcgaca
     3541 ggcagggcgc gcgcccgtgg gagcgcgaca ccctcgtccc tgtcttctca gccaccaagg
     3601 cgcccgccgc agtctgctgc cttctggccc tgcatgaggc aggctttccg ctcgactgcc
     3661 ctgtggccga ggcctggcct gagtttgtgg gcggcggcaa gtcgcgcatg cacttcgccc
     3721 acctgctctc ccacacctcc ggtttgtgcg cgctggatga aaaagcgccc atttataatt
     3781 acgacgccgt catccaggcc ctggaaatgc agcccccgct ctgggaaccc ggcacccgcc
     3841 agggttacca cgcccgcacc ttcggcttcc tcatggacga gattgtccgc cgcgtcaccg
     3901 gagccgactc cctcggccag ttctttcgcg acacatttgg cgatcccatg aatctcgact
     3961 tttggatcgg cctgcctgag cagcagtggc cgcgcgtctc acccgtgcat ccggggaaaa
     4021 tcagcatcgc cagcggcgac cagccctttc tcaaagcctg gaacacctcc ggcacactca
     4081 cccagcgcgc ttttagcagc cccttcggcc tcagctcgat cagcgagtac aatcaatccg
     4141 ccttctggtc ccccggctat gccagcatgg gcggcgtcgg cagcgcgcgc ggccttggca
     4201 aattctacgc catgctcgcg cagggcggct catggggagg cgcgcgcctc gtgccagccg
     4261 ccgtcctcgc gcaactccag cacaccctct cccaggagga ggacgccgtg ctgctcacac
     4321 ccgccgcctt cgccgccggc gtcatgcagg acccgctgga ccccgacccg gagtccggcg
     4381 gcggcaagct gcgccgccat ttcggcccct ccctctccgc cttcggccac ccaggcgccg
     4441 gcggcagcct cgccttcgcc gatccggaga acggcctcgc cttcgcctat gtcatgaacc
     4501 aaatggaggc cggccccatg cccggcgcca aagtcctcgg cattgtcgag cggctctacg
     4561 catgatccct ccaccatgag ccttccacac atcgccgtca tagactggcg cgcctccccg
     4621 cagggtgacg acgactgctc catcgagcgt tccgtcatcg gctccaccgc ccgccttacc
     4681 cgccacctct gtgacaccga cgccgacctc gacgacgagg ccgccggtgc cgatgccatc
     4741 atcctctggc acaacatgcc tctcaccgcc cgcggcatct cccgcctcgt caaatgccgc
     4801 gccatcatcc gcaacggcgt cggttatgac agtgtggaca ccaccgccgc ccgcgcgcgc
     4861 ggcattcccg tctgcaacgt gcccgactac ggcaccgagg aggtggccga ccacgccatc
     4921 gcgctcgcgc tcgcgctctg ccgtcagctc tttccgcttg atgccgaggc caaggcgctc
     4981 ggctgggaga tcaaggtgcg cgggaagctg cgccgcttgc gcggcctgaa cttcggcatc
     5041 gtcggccttg gccggatcgg caccgccacc gccttgcgcg ccaaagcgct cggctttcgt
     5101 gtcgtttttt atgatccctg cctgcccaac ggagcggaca aggccctcgg catcgagcgt
     5161 gcttggagcc tcgatgagct gctcatgcag gccggcgtgc tctccctcca ctgcccattg
     5221 aatgacacca cgcgccacct catcggcgcg cgcgaactgg cattgctccc ggagtccgcc
     5281 cacgtcgtga acaccgcccg cggcggtctc atagacaaga ccgccctcct cgccgccctg
     5341 cgcgagggcc gcatcgccgg agccggtctt gacgtcatcg aggacgagcc cctgcgcact
     5401 cccgaggagg ccgccacgcc caacctcatc gtaacctgcc acgccgccta ctacagcggc
     5461 atgtccgtcg tcgaaatgag ccgcacctcc gcccgcatcg ctcttgccgc cgtcaccggc
     5521 gcgcccctgt tcaatgtggt gaacggagtt gtgtgagcag gaagtctcac ttgagcggac
     5581 ctttcagatt ccacgcttgc cgatgtgcca gccaaggtcc gtgcgaattt gaaggaaccg
     5641 gcgaaaaggc cggatgatcc cgtcaatacc ctggaggttc ttgccgaaat cctgaaggga
     5701 ttggaaaaat ccgggagggt attcaggctg gtcatcagcc gccggggtag agctgcggcg
     5761 gacgctcggc aaccaggagc ctgtcggcag aaagcggcgg gatcgcgtga tttgcgaagg
     5821 gtgtgagcct tttgatggac ggctgggacg gagcacgccc tccgcagatg cgggcaaagt
     5881 cagcggcgct gctccttgtg gcgtgcgaag agggcgaggg tgcggtcccg ctcggcggcg
     5941 tggtccacga tgggggcggg gtagccgggg gcgacggggc gtccgtcttt gggcggatca
     6001 agcaggagat cggcgggcag gccgcgcagt tcgggcaccc aggtttggat gtagcgcgct
     6061 tgcgggtcgt ggcgcttgct ctgagtccag ggattctgga tgcggaagta gggggcggca
     6121 tcggctccgg tgcctgcgct ccattgccag ccgccgttgt tgctggcgtt ttcaccgtcc
     6181 acgaggtggc gcatgaagaa gctttcgccc aggcgccagt cgcagtggag gtccttggtg
     6241 aggaacatgg cggtgatcat gcgcaggcgg ttgtgcatga agcctgtggc cagaagctgg
     6301 cggatgcccg cgtccacgat ggggaagccg gtgcggccct ggcaccaggc ctggaagtgg
     6361 gcgtcggtgc cgggccagtg gaggccgcga aagtcggggg agaactcttc ctcaaaaacg
     6421 ccggggtagt gccagaggat ggcgaggtaa aactcccgcc agacgagctc cttgatgtag
     6481 gtctcgatgg agagccgcgc ctgcggggag gagtcggcgg ccatcgcctc ctggcagcgg
     6541 gagtgcagct cgcggatcga gatgaggccg aagcgaaggt cctgtccgag gcgggaggtg
     6601 gtgacgccct cgggcacgtc acgccggggc ttgtaatcgt gcagcacgcg gctctcgatg
     6661 aaggtcttca tgcgctggcg ggcggctttt tccccgggtt cggggatggc ggctgtgcat
     6721 tcggggagct gccaccaggc cagagagggc aggggcaggg aaggtaaagc aggggcaggg
     6781 ccgagagcct tgacgcggcc ttcgggccgg ggcttttcaa gtgcgagcca ggcgcgcgag
     6841 tagggggtgt aaacccggta ggggccgccg gctccggtga gggcctcctc cggttcgtgc
     6901 aggacgcggt ctttgtggtc atggcaggcg actccgagcc tggcgcagag ggcgcgaact
     6961 ccggactcgg cggcgcggcc aaaggggtcg tagtcacgat tgaaaaagac ggcgctggcg
     7021 ccggtctcgt ggatgagctt ttccaggacg gcttcggcgg tgccgctgcg caggctgaga
     7081 cggctgccga tggcttcgag atttttcgcc agggaggcga ggctgccgca gaggaagtgc
     7141 tggcgtgcgg ggccggtcca gtcgtggtgt tttttccagt cgctgaggat gtaaagcggg
     7201 atgacgccgg cggcggagcg ggcggcgtgc agaagggcgg gattgtcagt gaggcgcagg
     7261 tcgcggcgga accagtggat gacgacttga tgagcgggca tgagtctggc ttacgcgtgc
     7321 tgatgcggtt gggacgctgc gggtgcggtg atcccggctt ccattaggct gtggtggatg
     7381 cgtcggctgc tcggtcttca gtcagggacg atgaggtgag cgcctccgct gatgaggccg
     7441 tcggggccgg gcaggagata gtatccgtgg ccctggccgg ggtcggcgcc gggcaggcgc
     7501 acgaaggcgc cggtgatggc ggcgcggcgc gcggggctgc cgggcagggt gaaactgccg
     7561 gtgaaggtgc cgcggcgggc gttgacctgg aggctgaggc ggttgggatt gtcgccaaag
     7621 acgcggagag tgctgggggc caggagggtg aggggctggc ggaacagttc ggtgacgtcg
     7681 ttctcggcga agacagcctc cgcattgccg tcacccgccg gggcggagag gaagttgaat
     7741 agatgactga ccggcgtgta tctgccgccg tgcgcgccga gcatgagggt gggcaacccg
     7801 gcgcgacggt gctcgggtgt gccggaaagg ccggcgttgg tgtcgtcaag gatgagccag
     7861 ccctgggcgc tgccgctctt tttggccacc agggcatgga aggccagacg gccgccgagc
     7921 agatggggtg tggagcaggt gagggtctgg ccgtcgccaa ggcggccggt ccaggcgatg
     7981 aggcccttgg cattgagcgt gccggcgatg tggccgctac cctgggggga ggcggggtct
     8041 tcggcaaagg cggggtcggg tttgaaataa gcggtgtgct cgccggaaag gactgtctgg
     8101 agggcgggat tccaatcgtg acggcgggcg gtgaatccgg cggggggctt ttcgccatca
     8161 tcaaggctgc cggtgatgat gccgtcctgg acttcgaaag cgagggtgac ggggggcctg
     8221 ccgccggcgc gcttgatgat gacggtggct tctggggggg ctgccccgga gggcagggcc
     8281 ttgaggcggt cggagaagcg caggctctcc gcgccgaggg tgaggttccc ggagaaggcg
     8341 ccggtgggtg tgagggtggc ggtaaatcgc ccgccgaggc cgccagtgag ttcggtgccg
     8401 ccgtccacga gaccgtggta agcgccgggt gtgccgtcgg gtagcggctc gacctgccag
     8461 aggaaggtga gcggctggct ggcgccgctg gcattaaagg cggtgatgat gatctcgtgg
     8521 gtgtcgggca ggtcgggtgt gccgctgagc atgccggtgg tggtgctgaa cttcatgccc
     8581 ttgggagttt ttttgatgct gaaacgggtg gcgccatgag cggcggtgaa ctgcagggcg
     8641 gccgcgccgc tgacgatggc ggggacgacg gcggggggct gcaccacagg cttgaggcgg
     8701 acatcaagct gggcgggcag ggtatgcaac tcgacggtgc cgggcgggtc aaagctggcg
     8761 cggcaggtgt aggcgccgat gtctccagcg gcggtgtttt tgatcgtcaa ggtggcggag
     8821 ccggaaccgg tgacgcgtcc gtcgtcgttg agcggggtgc catctcggaa ccaacgcagg
     8881 gagaggcctg ggcctgccgc ggcagtcttg agcacggcgg tcttgccctc ggcgacggac
     8941 tgcccggcga cggtgcggcg gagcacgcca agggcggctt cggggctggt ttcggtgccg
     9001 gtggcattgg tggcagtgac ggtgtagccg ccggcgtcgg cgagcttggc gctattgatc
     9061 gcgaaactgg cggagccatc accgagcacg gtggcggcgc cggttttgcg ccattggaag
     9121 gtgacgtggc cgccgccctc gcctgcggcg gtgaaggcgg cggtttcacc ttcaggaacg
     9181 atgacgggca ctggctgcag ggtgagcgcc gcaggctgga cctgggagcc gtaaagccgc
     9241 acttccgcgc tatggagggt gccggtgtcg ccccgcgcga ggtcctggca gcgcagggtc
     9301 cacagaccgc tcgcggactc gccccaatga cggacgctgg aaaaggtcca gccgtcgtag
     9361 tcgaggccgt cgtcctggga ggttttggcg gcgagggtgc tgacggtgcc ggctggggag
     9421 gtgagggtga tgtggaggtc accgcggaag gtgtgggcga tgtcgagcgt gacggtgacg
     9481 tgctcgacgc gcaggtcggt gacattggaa aaatcgaagg tgcgggtgac accggcggcg
     9541 ttgttgtcgg gtatgatgac gttcggttcc aggtcgcggg cgtcttcgat catggggccg
     9601 agattttccc aggtttccgc catggccacg gcggcctggg cgctgaccgc gccgccgccg
     9661 taaagctcgt ggtgcttgat ggggggcatg gatggagggg cgccgccggc gcgggtgacc
     9721 caacctgcct gggtggggaa gatgtgggtg gaggagcgca ggaggatctc tttgacatcg
     9781 cgccagccaa gctggggatt gacctcaagc atgagcgcca gcacgccggc gacgacgggc
     9841 gcggaggcgg aggtgccgcc aaaggtctgg gtgtagtcgc gcatctccgg ggagccggtc
     9901 aactctccgg gcttcgtccc cgtgttgtat ccctgggcgc cgcgatagtc ggtggtcacg
     9961 acgccggtgg cgccgggggc gacgacggtg aggtggctgc cgccctcgct gtagtttgcc
    10021 agggcgcctg tctgggtgag cacgccgacg gcgatgccat agatggagtt gggatagctg
    10081 tctttgttgc cctggtcgcc gcggtcgcgg tcattgcctg cggcccaaac actgagagtg
    10141 cccagcccgc ccctcccctc aagggcggct gtgcgcctgg cggcgacggt gagcggtccg
    10201 ctccagccga gttcggggaa gaactctgga tgcaggatgg tgggcgcggc cccccaactg
    10261 ttgtttttga tctggatgac gtcattgccg cgcagcatgg cttccgcgtc gtcgctgtcg
    10321 tctgtgggca aagagatcag gcgaaacccc accagcgtgg cctgcggagc cacgccggag
    10381 actccgaggc tgttgccacc gcgcgccgcc gccagtccag ccaccgccgt cccatgaaag
    10441 tcttcgttca ggaagtttcc ggtcggatca ttgtcctgcg gggtgtcatt ccaatcccag
    10501 tggttgtcct gcgagtcaag gttgggaagc aggtcgggat gcaacatgtc gagaccgtca
    10561 tccacgatgg cgatgcgcac gccctggccg gtgcctccag cggaccagac gggcagcact
    10621 tgaatgtccg cacccggttt ggaggcgctg gcggcggcgg ctttcaggtg ccactgcctg
    10681 aagaaaaggg ggtcgttggg atcggcgtgc ttgtgctttt gcctgagcag caggggcgag
    10741 gccgagacga tggccgtgtg cccatgcagg gcggcggcgg ccttcaatcc cccctcggcg
    10801 ccgccggcgc gcgcggtgac gataagttgc ccgcgcgcgt agtccggctc ggagacgagg
    10861 tcaaagcccg ccgcgtccaa ggcggcgagg gctacggcgc ggtcacggta ttgcagcagc
    10921 catttgccgg tgagcacgac gaggccggcg ctgtcatcgc ctgcgcgagg cagggcgggg
    10981 ttttctttgg gcgcatgact caccctggcg agacgcagca ccaggccgga ttggtctccg
    11041 agtcgggcgg cctcccgcag cagtgtggaa gcatcggccg cgtggacggg aatccagcgc
    11101 tgggccaccg gaagcgcgcg cgaatacaag cggtcgagca gcagttccat gcgcaggggt
    11161 ctgccgtttt ccatcagaag cacgggccgc ccggcataaa ggtccgccag cgcgcggtgc
    11221 tcccagtttt cctccgccaa cattgcggcg gtttttcttt ccatgctcaa tgccccttcg
    11281 gcacccaccg gcccatgctc ctcccgcagc caccatgcgg cagccagggc caggatcagc
    11341 aggaccggga ccatgacatg ccacacatga tgtttggagg gaatattcgg cattaaggcg
    11401 atcctatgag tttccagagg ggaaggcaag acgaggatgt cttgaccttg tattcacacg
    11461 ctttgatcaa aattggccca aaagttgtgt ggaggaacgc caattctcac gcttcaaatg
    11521 cctgacggca tccttgcctc ttatatcccg ggctggtcgc accgaaatga acaccagctc
    11581 ggatcaagga cgtgtgaccc ttttccagaa gaaatccagc tcccgcgcaa gcacgctcat
    11641 cacttgcgcg ggttcggcct ggaggacgcc gctttcggct tctttaatga tgcgctgcat
    11701 ggcgtagagc gcggc
//
LOCUS       DAOMYJ010000166        11678 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Prosthecobacter sp. isolate AMR_MDS_5629
            NODE_1835_length_11678_cov_23.604835, whole genome shotgun
            sequence.
ACCESSION   DAOMYJ010000166 DAOMYJ010000000
VERSION     DAOMYJ010000166.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867850
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prosthecobacter sp. (wastewater metagenome)
  ORGANISM  Prosthecobacter sp.
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Verrucomicrobiaceae; Prosthecobacter.
REFERENCE   1  (bases 1 to 11678)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11678)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.30
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:34:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,742
            CDSs (total)                      :: 4,684
            Genes (coding)                    :: 4,676
            CDSs (with protein)               :: 4,676
            Genes (RNA)                       :: 58
            tRNAs                             :: 55
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11678
                     /organism="Prosthecobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1835_length_11678_cov_23.604835"
                     /isolate="AMR_MDS_5629"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1965333"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(105..443)
                     /locus_tag="PLP58_15655"
     CDS             complement(105..443)
                     /locus_tag="PLP58_15655"
                     /inference="COORDINATES: protein motif:HMM:NF014019.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjQ family protein"
                     /protein_id="HRJ10014.1"
                     /translation="MPPPPPLLLSNIESLPGYRVTACLGLVQGSTIRAKHFGRDIMAG
                     LKNLVGGELRGYTELLQESRQEATSRMMEQAARLGANAVINVRFGTSSITQGAAEILA
                     YGTAVRVEPA"
     gene            664..1449
                     /gene="mazG"
                     /locus_tag="PLP58_15660"
     CDS             664..1449
                     /gene="mazG"
                     /locus_tag="PLP58_15660"
                     /EC_number="3.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009965873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphohydrolase"
                     /protein_id="HRJ10015.1"
                     /translation="MIPDHPDALTRLRAIVHRLRAPGGCPWDREQTHETLVPHLLEEA
                     YEVVDAIRSGDASLLCEELGDLLLQPILHAEIAAEEGRFDLDQMAAVLSEKLIRRHPH
                     VFGEADAATSAAVLNQWDAIKRQEKGTQKEGHLHGTGAGLPALMRAQKLQKKAARAGF
                     DWPDTAPVFDKIQEETAELRKAMAAGDRAGIEDEIGDLFFSVVNLARKLGIESEAALA
                     AANEKFTRRFHAVEQRLAAEGKSLEAATLAEMDAAWDAIKKEA"
     gene            1446..2219
                     /locus_tag="PLP58_15665"
     CDS             1446..2219
                     /locus_tag="PLP58_15665"
                     /inference="COORDINATES: protein motif:HMM:NF014724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HRJ10016.1"
                     /translation="MKTLIYRALDLMQPLGLCWLLLTAWVALAWCKRRPGKALPTLAW
                     LVLSLATCTPLPSWLMAGLESRHAPVTIGALPQADMIVCLGGGAEPSFIEPTGIHLKG
                     AADRLSTALALALRKKAAALVLGGGGYSQDGEDHSEADAFVQHLSRLGEMPVEVVSLG
                     LCSDTHDEAVKCAALARERGWRSVLLVTSASHMARSFATFEKAGLEVIAVPCAYESSF
                     HRVGDMRWLHLPHAGSFSIFASWFHEVIGMAAYRWRGWM"
     gene            2338..3351
                     /locus_tag="PLP58_15670"
     CDS             2338..3351
                     /locus_tag="PLP58_15670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007413671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase family protein"
                     /protein_id="HRJ10017.1"
                     /translation="MTRRKGILAGGNFIMDHVKLINAWPEQDMLADIQKETSSNGGGP
                     YNVLKDLAAMRVSYPLEAAGLVGKDANGDWILRDCKAAGIDAAQLRQTDRAPTSYTDA
                     MTVAGTGRRTFFHQRGANALLGEADFDFTRTRARLFHLGYLMLLDEMDRLLPGGGTPA
                     ARVLAAAQKAGLLTSVDIVSTENPDFRAIAEAALPHTDFLVINEVEAGKVAGVSLKTN
                     PVNVSAVCDAARALLERGVQRQVVIHFEAGAVVADRSGEVSIQASLNLPEGFIAGATG
                     AGDAFAAGYLHGVHEGFSTADSLTLAVCTAAACLTHPAPSMGLRPVAECLRLADEHGF
                     RAL"
     gene            3532..4365
                     /locus_tag="PLP58_15675"
     CDS             3532..4365
                     /locus_tag="PLP58_15675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009963232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein phosphatase 2C domain-containing
                     protein"
                     /protein_id="HRJ10018.1"
                     /translation="MEPTPSPDEPPQRLSWSGMTHVGRLRSNNEDAFLALTYNDREVR
                     YLGKTGASCFDDGDFVFAVSDGMGGANAGEFASRIAVERITRIMPRLYRLAAQNLGAG
                     VSDALEDIFHQVHAELGRMGRFYEECAGMGATLSMAVFCPGWMHFCHIGDSRIYYLPA
                     GGGMRQVSHDHSHVGWLRREGKINEREARSHPRKNVLNQALGAGHQILSPQFGSVSCH
                     SGDRFLLCTDGIVDGLWDSRLEEYASTPAAQPKAFRIVEQAVAESGRDNCTAVLVEFA
                     A"
     gene            4463..5788
                     /locus_tag="PLP58_15680"
     CDS             4463..5788
                     /locus_tag="PLP58_15680"
                     /inference="COORDINATES: protein motif:HMM:NF041199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trx7/PDZ domain-containing (seleno)protein"
                     /protein_id="HRJ10019.1"
                     /translation="MKTAFLPLLALLAASLPLPAETVKDREGAVRADKARFENDARWA
                     YNDIESGFKLAQATGKPLLVVMRCVPCLSCMGIDTAVLMENDALSALLDQFVCVRVMN
                     ANALDLTKFQFDFDLSFSTLFFNGDGTVYGRYGSWTHQKNSQESSLAGYKRALEAALA
                     VHKNYPANRAALQGKQGAPLPFVSTLDIPQLAERYQRELNWDGKVVQSCVHCHMIGDA
                     LRNTYRREKKPIPAEWIYPMPAPETVGVTLAQDQVAAVAAVIPGSHADKAGVRAGDEI
                     THVQGQPLVSVADFSWALHRTADQASLELAIKRAGAATTATLVLPSGWRHDTDSSGRV
                     AAWPMRGMALGGMILTDLDDSERRAAGLDAGVMALRVKGLGMYGLHAAAKKAGVQKDD
                     ILVEMDGHSRRMTESSLLGHLLQNRFPGETVQAVFLRGGERVTIRLPMQ"
     gene            complement(5945..7243)
                     /locus_tag="PLP58_15685"
     CDS             complement(5945..7243)
                     /locus_tag="PLP58_15685"
                     /inference="COORDINATES: protein motif:HMM:TIGR01891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004180 - carboxypeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HRJ10020.1"
                     /translation="MKILAAVLALPALALAADLEALTRAEMPSLLALYQELHANPELS
                     LHEVETAARIASELRAAGFDVTEKVGGQGLVGVLKNGDGPVIMVRTDLDALPVKEQTG
                     LPYASTRTVKDEQGREVHVMHACGHDVHMACFIGAARVLAKTRDTWRGTLVFVGQHAE
                     ERVLGARLMLRAGLFSKFPKPGHALALHCSADMAHGAVGIVEGYALANVDTLEIVVKG
                     VGGHGSMPHLARDPVLLASQIVVALQSIVSREVRPGDPAVVTVGSIHGGTKSNIIPDE
                     VRLQLTLRSYRAETRAHLIESIRRIVKAQAESARMPAEKMPEVHVSDDQAPALYNQPE
                     LCARVRRSLLPVLGKAGVLAREPVMGAEDFAEYGLTREKVPLCMFWLGTQAPETVAKA
                     SAEGRTLPSLHSPFFHPVPEPTISTGVKAMSAAVLDLMGK"
     gene            complement(7316..10189)
                     /locus_tag="PLP58_15690"
     CDS             complement(7316..10189)
                     /locus_tag="PLP58_15690"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012419951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003994 - aconitate hydratase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aconitate hydratase"
                     /protein_id="HRJ10021.1"
                     /translation="MNTLSTFTTGNGAPGKFFSLTELEKQGVGKVSKLPVSIRIVLES
                     LLRNLDGKKVTERDVTNLANWNAKAPGDYEIPFTVARIVLQDFTGVPLLVDLAAMRSK
                     VHELGRDPKMIEPLVPVDLVVDHSVQVDFAGTQEALNKNLALEFERNRERYEFLKWGE
                     QAFDTFKVVPPGIGIVHQVNLEYLAKGVLEKDGVFYPDTLVGTDSHTTMINGLGVVGW
                     GVGGIEAEAGMLGQPVTFLVPEVVGVHVTGALREGVTATDLALHCTQMLRKHGVVGKF
                     VEFYGPGAESLPLPDRATIANMAPEYGATMGFFPIDEESVNYLRGTGRSEELCATFKN
                     YFTAQGMFGIPQKGEIEYSSDLELNLADIQPSVAGPKRPQDRITVDALKSTWNEILTK
                     PTPQGYGKQPEINIGAPEELPETLDTVPDASPLSQALGAQEGVVIEENEDPAYPLWEV
                     SVDSKGGEKDVITHGSVLIAAITSCTNTSNPSVMLAAGLLAKKANAKGLTVKPAVKTS
                     LGPGSRVVTDYLTKTGLQPELDKLGFQTVGYGCTTCIGNSGPLDPGIEDVVRSEDIVA
                     ASVLSGNRNFEARVHQSIKANFLMSPPLVVAYAIAGTVDIDLSKDEIVAGSGVYLKDI
                     WPSLEEIREVMLAALSPEVFRALYTDFASQNPKWNEIPGSTGHVYGWNDKSTYIQHPP
                     FFEDFSMEPRDIVEITGARPLGIFGDSVTTDHISPAGAIKKTSPAGRFLEENGVPQAD
                     FNSYGSRRGNDRIMTRGTFANVRIKNLMVPGTEGGITRFFDPGQALGTSEGSGGSCCT
                     PPPIPGQGADVVSIYDAAVCYQAAGIPTIVIGGEDYGMGSSRDWAAKGTRLLGVKAVI
                     TKSFERIHRSNLVGMGVLPCNFKDKADYDKVKDLADATFDILGLSNDFKPMSEATLHV
                     KPANGAPFDIPLVVRIDTPVEIDYYRAGGILPYVLAQILRANG"
     gene            complement(10323..>11678)
                     /locus_tag="PLP58_15695"
     CDS             complement(10323..>11678)
                     /locus_tag="PLP58_15695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009960031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1501 domain-containing protein"
                     /protein_id="HRJ10022.1"
                     /translation="ALAADPHAARRPHFAPKAERCIFIFLEGGPSQMDLFDPKPLLNK
                     YDGQPLPDSIVGDAKFAFLQKDTATVMGTKRVFQKHGQCGMEISDLLPRIATCADDIA
                     LIRSMHTTQFNHLPGQLMLNCGAPLLGRPSVGSWLAYGLGNASRELPSYVVMVSKGRG
                     LPGGSSMWSSGFLPSSYGGVMFRNGETPVLNLPNPEGITREMQRASLSALNDLNHLRH
                     KHIGDPEIAARINSYELAFRMQSAAPELVDLKGESAATLDAYGVNRVEPPIKSNLGGI
                     GNYQIFSRHCLQARRMVERGVRFVNIIHASWDHHSSLDPQLAHNCGMVDQPIAALLKD
                     LRQRGLLDTTLVVWGSEFGRTALGENRPGNKSVTGRDHHPYAFSLWMAGGGIKGGQVY
                     GGTDDFAWHVARDPVSIHDFHATILHLFGMDHTRLTHRFQGRDFRLTDVHGEVVKGLL
                     A"
BASE COUNT         1927 a         3709 c         3981 g         2061 t
ORIGIN      
        1 ctcgcgctcc tcgatgccgc ggaagtggcg gccctcggcc atcttgccga agacaaggcc
       61 aagaatgagc agcagaatga aaatctccat gcgcgcggaa tggatcaggc gggttcgacg
      121 cgcaccgccg tgccataggc gaggatctcg gcggcgccct gggtgatgga ggaggtgccg
      181 aagcgcacat taatgacagc gttggcgccc aggcgcgcgg cctgctccat catccggctg
      241 gtggcctcct ggcgcgactc ctggagcagc tccgtgtagc cgcgcaactc gccgccgacg
      301 aggtttttca gcccggccat gatgtcccgc ccgaagtgct tggcgcggat ggtgctgccc
      361 tgcaccagcc ccaggcaggc ggtgacgcgg tatccgggca gggattcgat gttcgagaga
      421 agcagcgggg gtggtggcgg catggcgcgc gactcaacgt tccaccccgc cggatggcaa
      481 gcgcttttcc ccgctcgcgg cggcattttc gcgggaaaca ctccctcccc ggcaaaaagc
      541 gggcgtgcgc tgtcattggt atatcccatc ccgtgcttgc ggatgatctc gctgaagtcc
      601 ggcaggcaga cgcgccactt tcacgttttc attccgcctt ttgcggctat cgcctgggct
      661 gtcatgattc cagaccaccc ggatgccctc acccgtttgc gcgccatcgt ccaccgcctg
      721 cgcgcgccgg gcggctgccc gtgggaccgc gagcagacgc atgagacgct ggtgccgcat
      781 ttgctggagg aggcatacga ggtggtggac gccatccgct cgggggatgc gtccctgctg
      841 tgcgaggaac tgggcgacct gctgctccag cccattctgc acgccgagat cgccgccgag
      901 gagggccgtt tcgatctcga tcaaatggcg gcggttttga gcgagaagct catccgccgc
      961 catccgcatg tctttggcga agccgacgcc gctacctccg cagcggtgct caaccagtgg
     1021 gatgccatca agcgccagga aaagggcacg cagaaggagg gtcacctgca cggcacgggc
     1081 gcggggctgc ccgcgctgat gcgcgcgcag aagctgcaaa agaaagccgc gcgcgccggc
     1141 tttgactggc cggacacggc gcccgtcttc gacaagattc aggaggagac cgcggaactg
     1201 aggaaggcga tggccgccgg ggaccgcgcc gggatcgagg atgaaatcgg cgacctgttt
     1261 tttagtgtgg taaacctggc gcgcaaactc ggcatcgagt ccgaggccgc cctggccgcg
     1321 gccaatgaga aattcacccg ccgcttccac gccgtggagc agcgcctggc ggccgagggc
     1381 aaaagcctgg aggctgcgac gctggctgaa atggacgcgg cctgggatgc gatcaagaag
     1441 gaagcatgaa gacgctcatc taccgcgcgc tggacctcat gcagccgctc ggcttgtgct
     1501 ggctgctgct gaccgcctgg gtcgcgctgg cttggtgcaa acgccgcccc ggcaaggccc
     1561 tgccgacact ggcctggctg gtgctcagcc ttgccacctg cacgccgctg ccctcatggt
     1621 tgatggccgg gctggagagc cggcacgctc cggtcacgat cggggcgctg ccgcaggcgg
     1681 acatgatcgt ctgcctcggc ggcggcgcgg agccgtcctt catcgagccg accggcattc
     1741 acctcaaggg cgcggccgac cgtctctcca cggcgctggc gctggcgctg cggaaaaagg
     1801 ccgccgctct cgtgctcggc ggcggcggct attcccagga tggcgaggac cactccgagg
     1861 cggacgcttt cgtccagcac ctgtcacgcc tcggcgaaat gccggtggag gtggtcagcc
     1921 tgggactgtg ctccgacacg catgacgagg ccgtgaaatg cgcggcgctg gccagagagc
     1981 gcggctggcg cagcgtgctg ctggtgacct ccgcctcgca catggcgcgc agctttgcca
     2041 ccttcgaaaa agccgggctg gaagtcatcg ccgtgccctg cgcctatgag agcagctttc
     2101 atcgcgtcgg ggacatgcgc tggctgcacc tgccgcacgc cgggtctttt tccatcttcg
     2161 cctcctggtt ccacgaggtc atcggcatgg ccgcctaccg ctggcgcgga tggatgtgag
     2221 ggaatgcgga atgggtattt gggcttcaat tttcgaattt cgacatacat ccagcctccg
     2281 tattttcccc cgccctcggc ggattgacat ccggacggcg gccatccaac aacacttatg
     2341 actcgacgca aaggcatcct cgcaggcggc aacttcatca tggaccatgt gaagctcatc
     2401 aatgcctggc cggaacagga catgctggcc gacatccaga aggagacctc cagcaacggc
     2461 ggcggccctt acaacgtgct caaagacctc gccgccatgc gcgtctccta cccattggag
     2521 gcagccgggc tcgtcggcaa ggatgcgaac ggcgactgga tcctgcgcga ttgcaaagcg
     2581 gcgggcattg acgccgccca actccgccag actgaccgcg cgcccacctc ctacacggac
     2641 gcgatgaccg tcgccggcac cggacgccgc accttcttcc atcaacgcgg cgccaatgca
     2701 ctgcttggcg aggctgattt tgacttcacc cgcacccgcg cgcgcctttt ccacctcggc
     2761 tatctcatgc tgctcgatga gatggaccgc ctgctgcccg gcgggggcac gcctgccgcg
     2821 cgcgtgctgg ccgccgcgca aaaggccggc ctgctcacgt cggtggacat cgtcagcacg
     2881 gagaacccgg acttccgcgc catcgccgag gcggcgctgc cgcacacgga cttcctcgtc
     2941 atcaacgagg tcgaggcggg caaggtggcg ggggtgagct tgaagacgaa tccggtgaat
     3001 gtgagcgcgg tctgcgacgc ggcgcgcgcg ctgctggagc gcggtgtcca aaggcaggtg
     3061 gtcatccact tcgaggcggg cgccgtcgtg gccgaccggt ccggtgaggt gtccatccag
     3121 gcctcgctga atctgccgga ggggtttatt gccggcgcga ccggggcggg ggatgccttt
     3181 gccgccggtt acctgcacgg cgtccatgag ggcttctcca ccgccgacag tctgacgctg
     3241 gccgtctgca ccgccgctgc ctgcctcacg catcccgcgc cctccatggg cctgcgtccc
     3301 gtggccgaat gcctgcgcct ggcggatgag cacggcttcc gcgcgctgtg aggccggagg
     3361 cgctgtctca aagtggcacg gcttttggct ccgccccggc cgcggggagg ggactcacga
     3421 tgacaaatgg cgcgttcgag gctgcaagcc ctcacgggtt ggccggaggc aaaacccgcg
     3481 ccacaatgat cttgccgggg aagccaggac tgctccaaga tgcgcgcttt catggaacca
     3541 accccgtctc ccgatgaacc tccgcagcga ctgtcatggt cgggcatgac tcatgtcggg
     3601 cggctgcgct cgaacaacga ggacgccttt ctcgcgctca cttacaatga ccgggaggtg
     3661 cgttatctcg gcaaaaccgg ggcgtcatgc ttcgacgatg gcgattttgt tttcgcggtg
     3721 agcgacggca tgggcggcgc gaacgcgggc gagtttgcca gccgcatcgc cgtggagcgc
     3781 atcacgcgca tcatgccccg gctctaccgg ctggccgcgc agaatctggg ggcgggcgtg
     3841 agcgacgcgc tggaggacat cttccaccaa gtccatgccg agctgggccg gatgggccga
     3901 ttttatgagg aatgcgccgg gatgggtgcc acgctgagca tggccgtctt ctgccccggc
     3961 tggatgcact tttgccacat cggcgacagc cgcatctatt atctgcccgc cggcggcggc
     4021 atgaggcagg tcagccatga ccacagccat gtcggctggc tgcggcggga ggggaagatc
     4081 aacgagcgcg aggcgcgctc ccacccgcgc aagaacgtgc tcaaccaggc cctgggcgcg
     4141 gggcatcaaa tcctctcccc ccagttcggc agcgtcagtt gccattccgg ggaccgcttt
     4201 ctgctctgca ccgacggcat tgtggacggc ctctgggaca gccggctgga ggaatacgcc
     4261 agcacgcccg ccgcgcagcc caaagcgttc cgaattgtcg aacaggccgt ggccgagtcc
     4321 gggcgcgaca actgcaccgc cgtgcttgtg gagttcgccg cgtgatgccg gcgcggcata
     4381 cacccgctca agacggacag aaaggagtgt ctgtgccgca ttttgccaaa aggcgcgggc
     4441 gtggcgcgcg tttcaggcgg gcatgaaaac ggcattcctt cccctcctcg cgctcctcgc
     4501 cgcatcgctg ccgctcccgg cggaaaccgt caaagaccgt gagggggcgg tgcgcgctga
     4561 caaggcccgc tttgagaacg acgcgcgctg ggcctacaat gacatcgaga gcggcttcaa
     4621 gctcgcgcag gccaccggca agccgctgct cgtcgtcatg cgctgcgtgc cctgcctttc
     4681 atgcatgggc attgacacgg cggtgctcat ggaaaacgac gcgctctccg ccctgctcga
     4741 ccagtttgtc tgcgtgcgcg tcatgaacgc caacgcgctc gatctcacga agttccagtt
     4801 cgatttcgac ctctccttct cgacgctgtt tttcaatggc gacggcacgg tctatggccg
     4861 ttacggctca tggacccacc agaagaactc ccaggagtcc tcgctggccg gctacaagcg
     4921 cgcgctggag gccgcgctcg ccgtccacaa aaactacccc gccaaccgcg ccgccctcca
     4981 gggcaagcag ggcgcgccat tgcccttcgt ctcgactttg gacatccccc agctcgcgga
     5041 gcgctaccag cgcgagctga actgggacgg caaagtcgtg cagagctgcg tccactgcca
     5101 catgatcggc gacgccctgc gcaacaccta ccggcgcgag aagaagccca tacccgccga
     5161 atggatctac cccatgccag cgccggagac cgtgggtgtc acgctcgcgc aggaccaggt
     5221 ggccgcggtt gccgccgtca ttcccggctc ccatgcggac aaggccgggg tgcgcgcggg
     5281 cgatgagatc acgcatgtcc agggccagcc gctggtctcc gtggcggact tcagttgggc
     5341 gcttcaccgc acggcggacc aggcctcgct ggaactggcc ataaagcgcg cgggtgcggc
     5401 cacgacggcg acgctggtcc tgccatcagg ctggcgtcac gacaccgaca gcagcgggcg
     5461 cgtggccgcc tggcccatgc gcggcatggc tctcggcggc atgattttga ccgacctcga
     5521 cgacagcgag cgccgtgccg ccggcctcga cgccggtgtc atggcgctgc gcgtcaaggg
     5581 cctcggcatg tacggcctgc acgccgccgc gaaaaaagcc ggcgtgcaaa aagacgacat
     5641 cctggtggag atggacggcc actcccggcg catgaccgag agcagcctgc tcggccacct
     5701 gctgcaaaac cgcttccccg gcgagactgt ccaggccgtc ttcctgcgcg gcggtgagcg
     5761 cgtcacgatt cggctgccca tgcagtgagg cccggtgcgg caggccatgg gccgggcatg
     5821 ttcgcccaaa gtggcgcggg ttttgcctcc ggctgacccg tgtgggagtg cgctccgtgc
     5881 ctgccttgtg tcagcgagag tcaaaactcc cgcggcttgg gcggagccat aagccgtgcc
     5941 actttcactt ccccatcaga tcaagcacgg cggcgctcat ggccttgacc ccggtgctga
     6001 tggtgggttc ggggaccggg tgaaagaagg gggagtgcag ggacggcagc gtgcgcccct
     6061 cggcactcgc tttggccacg gtctccggcg cctgggtgcc gagccagaac atgcacagcg
     6121 gcaccttttc cctggtcagg ccgtattcgg cgaaatcctc ggcgcccatg actggctcgc
     6181 gcgcgagcac gccagccttg cccagcacgg gcagcaggga gcggcgcacg cgggcgcaga
     6241 gttcgggttg gttgtagagg gcgggcgcct ggtcgtcgga gacgtggacc tcgggcattt
     6301 tctccgccgg catgcgcgcg gactctgcct gcgctttgac gatgcggcgg atggattcga
     6361 tgagatgcgc gcgggtttca gcgcggtagc tgcgcagggt gagctggagg cgcacctcgt
     6421 cggggatgat gttgcttttg gtgccgccgt ggatgctgcc gacggtgacg acggcggggt
     6481 cgccgggcct gacctcgcgg ctgacgatgc tctgcagggc cacgacgatc tgggaggcca
     6541 gcaggacggg gtccctggca aggtgcggca tggagccgtg gcctcccacg cccttcacga
     6601 cgatctccag ggtgtccaca ttggcgagcg cgtatccttc gacgatgccg acggcgccat
     6661 gcgccatgtc ggcggagcaa tgcagggcca gcgcgtggcc gggcttcgga aatttggaaa
     6721 acaaacccgc gcgcagcatg aggcgcgcgc cgagcacgcg ctcctcggca tgctggccta
     6781 cgaagacgag agtgccgcgc caagtgtcgc gggttttggc gagcacgcgc gcggcgccga
     6841 tgaaacaggc catgtggaca tcatgcccgc aggcgtgcat gacatggacc tcgcggccct
     6901 gctcgtcctt cacggtcctg gtgctggcgt agggcaggcc ggtctgctcc ttgaccggaa
     6961 gagcgtcgag gtcggtgcgg accatgatga cgggaccgtc gccgtttttg agcacgccca
     7021 caaggccctg gccgccgact ttttccgtga catcgaatcc cgcggcgcgc agctcgctgg
     7081 cgatgcgcgc cgccgtctca acctcgtgca gggaaagttc gggattggca tgaagctcct
     7141 ggtagagcgc gagcagtgag ggcatctcgg cacgcgtgag agcctccaaa tcggcggcaa
     7201 gggcgagggc gggcagggcg aggacagcgg ccagaatttt catgcgcgca ttgtgccccg
     7261 ccgggcagca ctgcggcaag gggaaaaagg ggagtatgtt ttaaagctcc aaggcttacc
     7321 cgttcgcacg caggatctgc gcgaggacgt aggggaggat gccgccggcg cggtagtagt
     7381 cgatctcgac gggggtgtct atgcggacga cgagcgggat gtcgaacgga gcgccgttgg
     7441 ctggcttcac atgaagggtg gcctcgctca taggcttgaa gtcgttggag aggcccagga
     7501 tgtcgaaggt ggcgtcggcg aggtctttga ctttgtcgta gtcggccttg tccttgaagt
     7561 tgcagggcag cacgcccatg ccgacgaggt tgctgcggtg gatgcgctcg aaggacttgg
     7621 tgatgacggc tttgacgccg agcaggcggg tgcctttggc ggcccagtcg cggctggagc
     7681 ccatgccgta gtcctcgccg ccgatgacga tggtgggaat gccggctgcc tgataacaga
     7741 cggcggcgtc gtagatggat acgacatccg ccccttgccc tgggatgggg ggaggtgtgc
     7801 agcaggagcc gccagaacct tcggatgttc cgagggcttg ccctggatcg aaaaatcggg
     7861 tgatgccacc ctcggtgcca ggaaccatca gatttttgat gcgcacgttg gcgaaggtgc
     7921 cgcgggtcat gatgcggtcg ttgccgcggc ggctgccata gctgttgaag tcggcctggg
     7981 gcacgccgtt ttcctccagg aagcggccgg cggggctggt cttcttgatg gcgccggcgg
     8041 ggctgatgtg gtcggtggtg acgctgtctc caaagatgcc gagcggtcgc gcgccggtga
     8101 tctcgacgat gtcgcgcggc tccatgctga agtcctcgaa gaaaggagga tgctggatgt
     8161 aagtgctctt gtcgttccag ccatagacgt ggccggttga gccggggatc tcattccact
     8221 tcgggttctg gctggcgaag tcagtgtaaa gcgcgcggaa tacctccggg gagagggcgg
     8281 ccagcatgac ctcgcggatt tcctcaaggc tgggccagat gtccttcaaa tacacgccgc
     8341 tgcccgccac gatctcgtcc ttgctcaagt caatgtccac cgtgccggcg atggcgtagg
     8401 cgacgacgag cggcggggac atgaggaagt tcgccttgat gctctgatgc acgcgggcct
     8461 cgaagttgcg gttcccggag agcacgctgg cggcgacgat gtcctcggag cggaccacgt
     8521 cctcgatgcc ggggtcgagc gggccggagt tgccgatgca ggtggtgcag ccgtagccga
     8581 cggtctggaa gccaagcttg tcgagctcgg gctggaggcc ggtcttggtg aggtaatcgg
     8641 tcaccacgcg gctgccgggg cccagactgg tcttgacggc aggtttcaca gtgaggccct
     8701 tggcattggc tttcttcgcc agcagaccgg cggcgagcat gacgctgggg ttgctggtgt
     8761 tggtgcagct cgtgatggcg gcgatgagca cgctgccgtg ggtgatgaca tctttctcac
     8821 cgcccttgct gtccacgctg acttcccaga gcgggtaggc ggggtcctca ttttcctcaa
     8881 tgaccacgcc ctcctgcgcg ccgagtgcct gcgacagggg cgaggcgtcg gggacggtgt
     8941 ccagtgtttc gggcagttcc tccggcgcgc cgatgttgat ctccggctgc ttgccgtaac
     9001 cctggggtgt cggcttggtt aaaatctcgt tccaggtgga cttcaaggcg tccacggtga
     9061 tgcggtcctg cgggcgcttc gggccggcca cgctgggctg gatgtcggcg aggttcaact
     9121 ccaggtcgct gctgtattcg atctcgccct tctgcgggat gccgaacatg ccctgggcgg
     9181 tgaagtagtt tttgaaagtc gcgcacagct cctcgctgcg gccggtgccg cgcaggtagt
     9241 tcacgctttc ctcgtcaatg gggaagaagc ccatggtggc gccatactcg ggcgccatgt
     9301 tggcgatggt ggcgcggtcc ggcagcggca ggctttcggc gccggggccg taaaactcga
     9361 cgaacttgcc caccacgccg tgcttgcgca gcatctgcgt gcagtgcagg gcgaggtcgg
     9421 tggcggtgac gccctcgcgc agcgcgccgg tgacatgaac gcccaccacc tcgggcacga
     9481 ggaaggtcac aggctggccg agcatgccgg cctccgcctc gatgccgccg acgccccagc
     9541 cgacgacgcc gaggccgttg atcatggtcg tgtggctgtc cgttcccacc agtgtgtccg
     9601 ggtaaaagac gccgtccttc tccagcacac ccttggcgag gtattccaga ttcacctgat
     9661 gcacgatgcc tatgccggga ggcaccactt tgaaggtgtc aaaagcctgc tcaccccatt
     9721 tgaggaactc atagcgctcg cggttgcgct cgaactccag cgccagattc ttgttcagcg
     9781 cctcctgcgt gccggcgaag tccacctgca cgctgtggtc caccaccagg tccaccggca
     9841 ccagcggctc gatcatcttc gggtccctgc ccagctcatg caccttgctg cgcatggccg
     9901 ccagatccac cagcagcggc acgccggtga agtcctgcaa aacgatgcgc gccacggtga
     9961 acgggatctc atagtcaccg ggcgccttgg cgttccagtt ggccaggttc gtcacatccc
    10021 tctcggtgac ttttttcccg tccagattcc gcagcaggga ctccagcaca atgcggatgg
    10081 agacgggcag tttcgacact ttgccgacac cctgcttttc cagttcggtg agggagaaaa
    10141 acttgccggg ggcgccgttg ccggtggtga aagtgctgag ggtgttcatc gggtggtgga
    10201 agcgcagtga aagaatgcgg cagggaggaa gtcaagcgtg gatcacctgt cccaagcatg
    10261 aaaaacgcca actccagcgc cagctgccgc gcgccccgac catcacgtcc ttgcaggcgc
    10321 ggctatgcca gcagcccttt gaccacctcg ccgtggacat cggtcaggcg gaagtcgcgg
    10381 ccctggaagc ggtgggtcag gcgggtgtgg tccatgccaa agagatgcag gatggtggca
    10441 tggaagtcgt ggatggaaac cgggtcgcgc gccacatgcc aggcgaagtc gtccgtgccg
    10501 ccatagacct gcccgccttt gatgccgcca ccggccatcc acaggctgaa agcgtagggg
    10561 tggtggtcgc gtccggtcac ggatttgttg ccggggcggt tttcacccag cgccgtgcgt
    10621 ccgaactcgc tgccccagac caccagcgtg gtgtcgagca ggccgcgctg tctgaggtcc
    10681 ttgagcagcg cggcgatggg ctggtccacc atgccgcagt tgtgcgccag ttgcgggtcc
    10741 aggctgctgt gatgatccca cgaggcgtgg atgatgttca cgaagcgcac gccgcgctcc
    10801 accatgcgcc gcgcctgcag gcagtgccgg gagaatatct ggtaattgcc aatgccgccg
    10861 aggttgcttt tgatcggcgg ttccacgcgg ttcaccccgt aggcgtcaag cgtggccgcc
    10921 gactcgcctt tgaggtccac cagctccggc gcggcgctct gcatgcggaa ggccagctcg
    10981 tagctgttga tgcgcgcggc gatctccggg tcgccgatgt gcttgtggcg cagatggttc
    11041 aggtcattga gcgcggaaag gctggcgcgc tgcatctcgc gggtgatgcc ctccggattt
    11101 ggcaggttga gcacgggtgt ctcaccattg cgaaacatga cgccgccata cgaggagggg
    11161 aggaagccgc tggaccacat ggaactgccg ccgggcaggc cgcggccttt gctcaccatc
    11221 accacataag acggcagttc ccgggaggca ttgcccaggc cgtaggcgag ccacgagccg
    11281 acgctcggcc tgcccagcag cggcgcgccg cagttgagca tgagctggcc tggcaggtgg
    11341 ttgaactgcg tggtgtgcat ggagcggatc aaggcgatgt catccgcgca ggtggcgatg
    11401 cgcgggagca ggtcgctgat ctccatgccg cactggccgt gcttttgaaa gacgcgcttc
    11461 gtgcccatga cggtggccgt gtccttttgc aggaaggcga acttggcgtc gccgacgatg
    11521 gagtccggca gaggctggcc gtcatatttg ttgagcagag gcttcggatc gaagaggtcc
    11581 atctggctcg gaccgccctc caggaagatg aagatgcagc gctccgcctt cggggcgaag
    11641 tgcggcctgc gcgcggcatg cgggtccgcc gccagcgc
//
LOCUS       DAOMYJ010000167        11634 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Prosthecobacter sp. isolate AMR_MDS_5629
            NODE_1839_length_11634_cov_24.214699, whole genome shotgun
            sequence.
ACCESSION   DAOMYJ010000167 DAOMYJ010000000
VERSION     DAOMYJ010000167.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867850
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prosthecobacter sp. (wastewater metagenome)
  ORGANISM  Prosthecobacter sp.
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Verrucomicrobiaceae; Prosthecobacter.
REFERENCE   1  (bases 1 to 11634)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11634)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.30
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:34:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,742
            CDSs (total)                      :: 4,684
            Genes (coding)                    :: 4,676
            CDSs (with protein)               :: 4,676
            Genes (RNA)                       :: 58
            tRNAs                             :: 55
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11634
                     /organism="Prosthecobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1839_length_11634_cov_24.214699"
                     /isolate="AMR_MDS_5629"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1965333"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1284
                     /locus_tag="PLP58_15700"
     CDS             <1..1284
                     /locus_tag="PLP58_15700"
                     /inference="COORDINATES: protein motif:HMM:NF018530.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA C-terminal domain-containing protein"
                     /protein_id="HRJ10023.1"
                     /translation="PAILTGTITPTALGAAQQALQAAQADPAAVALPALAYAATYPLG
                     VAGIIASLMLLKRIFKVRVEEEAESFRQQQGAGVAPLERMNLVVQNPNLAGVAAAAIP
                     GIQETQVVISRIRHAGESAAHAATPQTLVQPGDVLLAVGTRAQLEQFRLVVGSETEEN
                     LMKAPGKVTYRRVVLTDPRILGKTVRELGLEHIHNVAVTRVSRGDLIFSAVPDLRLQY
                     GDMLQLVGEEEALNSATQALGNAVEQLQQTKFAAIFAGILAGVLLGLYPISIPGLPAP
                     VRLGLAGGPLLVAILLSHLGKIGPIIMHLPINANRALRELGILLFLAGVGLLSGAQFA
                     ETVFTAQGLRWVLLGVAVTLLPLLLVGIAARRLHRMNYMTLCGLISGGMTDPPALAFA
                     SQMARNDFPAVAYAAVYPLTMLLRIIAAQVIAQTG"
     gene            1307..1480
                     /locus_tag="PLP58_15705"
     CDS             1307..1480
                     /locus_tag="PLP58_15705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ10024.1"
                     /translation="MSRNCALLLRPVERLLSALLHGGHRWWPAGPSPEIYVGFILGLA
                     TFPAARVDEKASP"
     gene            complement(1487..2299)
                     /locus_tag="PLP58_15710"
     CDS             complement(1487..2299)
                     /locus_tag="PLP58_15710"
                     /inference="COORDINATES: protein motif:HMM:NF015297.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase/citrate lyase family protein"
                     /protein_id="HRJ10025.1"
                     /translation="MDARTLSQHLKEGRNVFGTLIVSPSPRWPEAVRGCGLDFVFIDT
                     EHIALNRAELSWMCQTYAALGLSALVRIPSPDPYAATMVLDGGAAGVIAPYVESVAQV
                     QALRGAVKLRPIKGKRLQQMLEGAKPEPELDAYIQEGAAKRLLIVNIESVPAIEALDD
                     ILAVPGIDAVLIGPHDLSCSLGIPEQWDHPRFLDACETIFRKARAAGVGAGIHFWGSV
                     EQQTRFLKLGANMLIHSADISLFQKHLRLELDAVRAAAGIASAKTSADTELI"
     gene            complement(2381..6694)
                     /locus_tag="PLP58_15715"
     CDS             complement(2381..6694)
                     /locus_tag="PLP58_15715"
                     /inference="COORDINATES: protein motif:HMM:NF012200.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choice-of-anchor D domain-containing protein"
                     /protein_id="HRJ10026.1"
                     /translation="MKLHSLAVFVIALIACMVPDARAQAASHEPAAPSAGKTPAGLNA
                     SDWQSIRAAYEAGRHAFMPVEGQDGHWQARNPGQQWTTRFDGRGFIATPREGGWTWGL
                     ELRSYGVGDKQTPVGASPPQVKAEDQRLTYDWDATVQEWWVNDQRGLEHGYVIATRPS
                     ALPPSSLSLVLATRGNLSPKIAADALGVHFTDDAGATVLNYTGLKVWDADGKTLPSRF
                     EAAGAGQVRLVVEDADARYPVTIDPIAQQAYMKRSDPRDTAGPGDGFGESVAVSGDTV
                     VVGARFEDSGTTGINSTPDELANNAGAAYVFVRNGTTWTQQAYLKAHQVTSGDFFGGS
                     VAVSGDTVVVGGYLEDSNTTGVNSTPNEGLGNTGAAYVFVRSGSTWTQQAYLKSHQVS
                     VGDNFGVSVAVSGDTVVVGANFEDSGTTGVNSTPDESATSAGAAYVFVRSGSTWTQQA
                     YLKAHQVSEFDQFGFSVAVSGDTAAISGVAERSGSTGVNSTPDESAVNAGAAYVFVRS
                     GVTWTQEAYLKPHQVNANDNFGNSVAVDGDTVVVGSRFEDSSTTGIDSTPNESAISAG
                     AAYVFARSGTTWTQQAYLKAHQVTAGDNFGTSVAVSGDTVVVGAYFEDSSTTGINSTP
                     NETATSAGAAYVFVRSGTTWSQQAYLKAGNAGQDDRFGISVAVSGDSVVVGASFEDSS
                     LTGINRTPNEAAPDSGAAYVFVRGGADWSQQAYVKPTPIPPGGAGPAAFDQFGASVAV
                     SGDTVVIGAPNEDSSSTGVNGIPNESVEDAGAAYVFVRDGGGAWSQQAYLKAHQVTAE
                     DYFGASVAVSGDTVVVGARNEDSSTTGINSTPNESAGNAGAAYVFVRDGGGAWSQQAY
                     LKASQVTADDQFGYSVAVSGDTVVIGTPGEDSSTPGVNSTPNESLGSSGAAYVFVRDG
                     TDWTQQAYLKAGLPGHEDEFGTSVSVSGDTAVIGAYSEDSFISGINSTPKKGISGSAA
                     GAAYVFVRNGTAWSQQAYLKAQQVTDGDAFGLYVAVDGDTVVVGAPYEDSGTTGVNSA
                     PDKSVADAGAAYVFVRSGTVWSQQAYLKAHQVTTGDQFGVSVAVSGDTVVVGALFDDS
                     ATTGINSTPDESAANAGAAYVFVRSGAVWIQQAYLKAGQVTAGDFFGGSVAVSGETVV
                     VGAANEDSASTGVNSTPNEDAGNTGAAYVFTGLGPPPEIALSGNGVDIASGDTTPAVA
                     DHTDLGGDLAGGSPVTRTFTITNTGSFDLILTGVPFVTLSGSTAFSVTQQPASAIVPA
                     HGGTRTFAVTFNPATLGTRTATVSIPSTDADENPFTFDVRATAFSTTADTDSDGLNDW
                     AEFSWSALGFDWEVSQPALVSALFSKLGNAPAHVNAAGFFSTAQVQALNVGVPLIQRD
                     PQTGTFTLTIGVAKSATLLPGSFDPFPMTAPQTIINGAGKLEFQFTVPDDAAFFQIRA
                     E"
     gene            complement(6776..7867)
                     /locus_tag="PLP58_15720"
     CDS             complement(6776..7867)
                     /locus_tag="PLP58_15720"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HRJ10027.1"
                     /translation="MFGVSTAAFAIGLPLLRVKRWNWLILILGAEWTHLSSGLTAAYG
                     WGGGFHFHLLLLTVVTLIFDHIPIRLRVLMALFPMAFFLGWFPPLMTRPPVIQLSAVQ
                     ANWLYVINALVFIAAAMVLVLHFIQSALVQKARAEALAESRAELIANLSHELKTPLAA
                     MLTTAQSTLKRAQAEADYREAILLWERNTREMSHLVVRMLDLVSADVHELKPQVRKVD
                     LVGLLGEVLLSFQDVARAKGARIEMDATGALPASTDPELLKIVLNNLLSNALRYCKEG
                     GAVRIEVRTEPGDCRISVIDDGPGIAPKDLPHIFDAFYRADRARSRSEGAKGLGLAIA
                     QRFAEALGAKISVQSEPGKGATFTLHCHR"
     gene            complement(8020..8688)
                     /locus_tag="PLP58_15725"
     CDS             complement(8020..8688)
                     /locus_tag="PLP58_15725"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HRJ10028.1"
                     /translation="MLILLIEDDLDLGAAIVRELKAEGFAVEWERTGSGGLFRAIEWD
                     HDLVILDRLLPGMDGTEVLRSLRKKKQVPVLMLTALNRLTDRVKGLDLGADDYLGKPF
                     ELAELLARIRALLRRSSAWSDEALTHGDLEFHFGAKRVFKGGKEVVLRASEFATVELL
                     LSRKGRPVSKQVLEERLHEDFGKFQSNSLEVHIHRIRSKLGHDFIQTKRGLGYLVPRL
                     GGDT"
     gene            complement(8862..10004)
                     /locus_tag="PLP58_15730"
     CDS             complement(8862..10004)
                     /locus_tag="PLP58_15730"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialidase family protein"
                     /protein_id="HRJ10029.1"
                     /translation="MKRIILFFLTAAASLIAADDYKGDVKDVRVVRHMDQHPGASLVW
                     EPYIAQWKPKHLVVAYGAGIPGKTDMGSIYASVSTNDGDTWSEAVPVFDHNHWQGAIH
                     FAYANAILFKPSDQEVMWCYAMRCPMNYRHSEDSQLVAAYTADGGRSWTPVELAMHYS
                     GPLIVVAGPIETKVKGKTRYLFPAHRNTLRNDPLGSRDQFVLSSTSLLEWKFEGYIPQ
                     PETGDKVFLHEGGIAVGDSPDELKIVMRTANWEEKGALDPPRAFSSISKDGGTTWTAA
                     KQEPELWNARSKGYFSRNADGSHLYVYNDGPAMPAKAGRTSLRYKVQPMGGAWSAEKT
                     FYDAGIKNSYPTLVEVAPGDFRAVWDSGTPDKARTHIHFGKIKIKN"
     gene            complement(10001..11185)
                     /locus_tag="PLP58_15735"
     CDS             complement(10001..11185)
                     /locus_tag="PLP58_15735"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="sialidase family protein"
                     /protein_id="HRJ10030.1"
                     /translation="MKGLATLLFVAAPLCAADFRVENTTVLTSSGAYHWAQSRPAFLP
                     GGRVIVTMQEIEKRGSHGYRDIYITETTDGAKTWSAPQRIAALNRKRFPDGIERVMGD
                     ICPQWHEKSGKLLVTGKCFGFLANPNDNKAKDDRSQERVAYAVYDPKSDVWSGMKIMA
                     MPEKGHAGNPIIEPNAGCHQRHDLPDGAVLLPVRYRADPKTRVYTSVVTRCAFDGETL
                     AYVEHGSEHTISIPRGLYEPSVCSFQGRYFLTMRGEQNGHVTRSDDGLDYQPTVEWKF
                     DDGQPLLSANTQQHWITHGEKLYLTYSRKGANNDHVFRNRAPIFIAEVDPEKLHIIRA
                     TEQILMPETGVDLTGGFAPVDVNANETWVISSEMAFPEARKDENNRVLLAKILWNQPA
                     KP"
     gene            complement(11248..11490)
                     /locus_tag="PLP58_15740"
     CDS             complement(11248..11490)
                     /locus_tag="PLP58_15740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ10031.1"
                     /translation="MITISLGQLIMLVMLIGVSFVCALWFYTFWREERREAMRRRIAI
                     QCRICGCNYAIPGKDRAITRCPACGSRNERGGLPQI"
BASE COUNT         2037 a         3783 c         3661 g         2153 t
ORIGIN      
        1 cctgccatac taacaggtac gatcacccca accgcactgg gcgcggccca gcaggcgctg
       61 caggccgcgc aggctgaccc tgcagcggtc gcgctgccag cgctggccta cgccgccacc
      121 tatccgctgg gcgtggcggg catcattgcc agcctcatgc tgttgaagcg aattttcaaa
      181 gtgcgcgtgg aggaggaggc ggagagcttc cgccagcagc agggcgcggg cgtggcgccg
      241 ctggaaagga tgaacctggt ggtgcaaaat ccgaatctgg cgggagtcgc cgcggcggcc
      301 atccccggta tccaggagac gcaggtggtc atcagccgca tccgtcatgc gggcgagtcc
      361 gcggcgcatg ccgctacgcc gcagaccctg gtgcagccgg gtgatgtgct gctggctgtg
      421 ggcacacgcg cccagctgga gcagttccgg ctggtggtgg ggagcgagac ggaggaaaac
      481 ctcatgaaag ccccaggcaa ggtcacctac cgccgggtgg tgctgaccga tccccgcatc
      541 ctgggcaaga ccgtgcgcga gctgggcctg gagcacatcc acaatgtggc cgtgacgcgc
      601 gtgagccgcg gcgacctcat cttttccgca gtgcccgacc tgcggctgca gtatggcgac
      661 atgctgcagc tggtgggcga ggaggaggcg ctgaactccg ccacgcaggc gctgggcaat
      721 gcggtggagc agcttcagca gaccaagttt gccgccatct tcgcgggcat cctggcgggc
      781 gtgcttcttg gcctgtatcc catctccatc cccgggcttc ccgcgccggt acggctgggg
      841 ctggcgggcg ggccgctgct ggtggccatc ctgctcagcc atctgggcaa gatcggcccc
      901 atcatcatgc acctgcccat caatgccaac cgcgccttgc gggagctggg catcctgttg
      961 tttctggcgg gggtgggcct tctttccggc gcgcagtttg ccgagacggt gttcaccgcg
     1021 caggggctgc gctgggtgct gctcggcgtg gcggtcacgc tgctgccgct gctgctcgtc
     1081 ggcatcgccg cgcggcggct gcaccgcatg aactacatga cgctctgcgg cctcatctct
     1141 ggcggcatga ccgaccctcc cgcgctcgcc ttcgccagcc agatggcacg caacgatttc
     1201 cccgcggtgg cctacgcggc ggtctatccg ctgaccatgc tgctgcgcat catcgccgcg
     1261 caggtcatcg cccagacggg gtgataggta gcagcagggc ccacacatga gccggaattg
     1321 tgccctgctg ttgcgaccgg tggagcgtct attatccgct ctgctgcacg gtggacaccg
     1381 ctggtggccc gccgggccga gtcctgagat ttacgtcggc ttcatcctgg gcctggccac
     1441 gtttccggca gcgcgtgtgg atgaaaaggc cagcccgtga gtgcgatcag atcagttcgg
     1501 tgtctgccga cgtttttgcc gatgcaatcc cggcagcggc gcgaacggcg tcgagttcga
     1561 ggcgcagatg cttttgaaac agcgaaatgt ccgcgctgtg gatgagcatg ttcgctccca
     1621 gcttcaaaaa gcgcgtctgc tgctccacgc tgccccagaa atggatgccc gcgccgacac
     1681 ctgccgcgcg cgccttgcgg aagatcgttt cgcaggcgtc gagaaagcgc ggatgatccc
     1741 actgctcggg aatgccgagg ctgcatgaca ggtcatgcgg gccgatgagc acggcgtcga
     1801 tgccaggcac ggcgagaatg tcatccagcg cttcaatcgc cggaacactc tcaatgttca
     1861 cgatcaacaa ccgctttgca gcgccttctt gaatgtaggc gtcgagttcc ggctcgggct
     1921 ttgcgccttc gagcatctgc tgcaatcgtt tgcctttgat gggacgcagt ttcaccgcac
     1981 cgcgcagggc ctgcacttgt gcgaccgatt cgacgtaagg cgcgatcacg cctgccgcgc
     2041 cgccatccag caccatcgtg gcggcatacg gatcaggtga tggaatgcgc accagcgccg
     2101 acagaccgag cgcggcgtag gtctggcaca tccagctcag ttcggcgcga ttcagcgcga
     2161 tgtgctcggt gtcgatgaag acaaagtcga gtccacagcc gcgcaccgcc tccggccagc
     2221 gcggcgaggg cgagacgatg agggtgccga agacgttccg gccttctttg aggtgctgtg
     2281 agagtgtgcg ggcgtccatg gaggtggtgc tcttgaactc ctcacacgca agcggccttg
     2341 ctggttcgtg gaattgacca acgactggac atccccgcgc ctactccgcg cggatctgga
     2401 agaacgcagc gtcgtccggc acggtgaact ggaactccag cttgcccgcg ccgttgatga
     2461 tcgtctgcgg agcggtcatg ggaaagggat cgaagctgcc cggcagcagc gtcgcgctct
     2521 tcgccacgcc gatggtgagg gtgaaggttc ccgtttgtgg atcacgctgg atcaacggca
     2581 cgccgacatt cagcgcctga acctgcgcgg tggagaaaaa gcccgccgcg ttcacatgcg
     2641 ccggagcgtt tcccagcttg gaaaagagcg cggacaccag cgcaggctgg ctcacctccc
     2701 agtcaaagcc cagcgcggac caactgaact ccgcccagtc gttcaacccg tcactgtcgg
     2761 tgtccgcggt ggtggagaag gccgtggcgc ggacatcgaa ggtgaagggg ttttcatccg
     2821 cgtccgtgct ggggatgctc acggtggcgg tgcgggtgcc gagcgtggcc ggattgaacg
     2881 tcacggcaaa ggtccgcgtg ccgccatggg ctggcacgat ggcgctggcg ggctgctggg
     2941 tgacgctgaa ggcggtgctg ccgctgaggg tcacgaacgg cacgcctgtg aggatgagat
     3001 caaacgagcc ggtgttggtg atggtgaagg tgcgcgtcac cgggctgcca ccggccagat
     3061 cgccgcccag gtcggtatgg tccgccacgg caggggtggt gtcgccgctg gcgatgtcca
     3121 cgccgttgcc gctgagagcg atttccgggg gcggacccag cccggtgaaa acgtaggccg
     3181 ccccggtatt gccggcgtcc tcattgggcg tgctgttgac gccagtgctg gcgctgtcct
     3241 cattagctgc tccgacgacc accgtttccc cgctcaccgc caccgagccg ccgaagaaat
     3301 cacccgccgt gacttgaccg gctttcagat acgcctgctg gatccagacc gcgccgctgc
     3361 gcacaaagac ataagccgcc ccggcattcg ccgcactttc atcgggtgtg ctgttgatgc
     3421 ccgtggtggc gctgtcatca aagagtgcgc cgacaacgac cgtgtccccg ctcacggcca
     3481 ccgagacgcc aaactggtcg ccggtcgtga cctgatgggc cttcagatag gcctgctggc
     3541 tccataccgt gccactgcgc acaaaaacgt aggccgcccc ggcatccgcg acactcttgt
     3601 caggcgcgct gttgacgccc gtggtgccgc tgtcctcgta aggcgcaccg acgacgaccg
     3661 tgtccccatc caccgccaca tagaggccga aggcgtcccc atccgtcact tgttgggcct
     3721 tcaaatacgc ctgctggctc caggccgtgc cgttgcgcac aaacacgtaa gccgctccgg
     3781 cagcggagcc gctgatgcct tttttgggcg tgctgttgat gcctgagatg aagctgtcct
     3841 ccgaataggc tccgatgacc gcagtgtccc cgctcaccga caccgaggtg ccaaactcgt
     3901 cttcatggcc gggcagcccg gccttcagat aggcctgctg ggtccagtcc gtgccatcgc
     3961 gcacaaacac gtaggccgct ccggaacttc cgaggctctc attgggcgtg ctgttcacgc
     4021 ccggggtgct gctgtcctcc ccaggcgtgc caatgaccac ggtgtccccg ctgaccgcga
     4081 cggagtagcc aaactggtca tctgccgtca cttggctggc tttcaggtaa gcctgctggc
     4141 tccaggctcc gccgccatcg cgcacaaaca cataggccgc cccggcattg ccagcgcttt
     4201 cattgggcgt gctgttgatg cccgtggtgc tgctgtcctc atttcgcgcc ccgacgacca
     4261 cggtgtcccc gctgaccgcc accgaggcgc cgaagtagtc ttccgccgtc acctggtggg
     4321 ccttcaagta ggcctgctga ctccacgctc cgccgccatc gcgcacaaac acgtaggccg
     4381 ctccggcatc ttcaacgctc tcattgggta tgccgttgac acccgtgctg ctgctgtcct
     4441 cgtttggcgc gccaatgacc accgtgtccc cgctcaccgc cacggacgcg ccgaactggt
     4501 caaaggcggc agggcctgcg ccgccgggcg gaatgggggt gggtttcaca taggcctgct
     4561 ggctccagtc cgcgccgccg cgcacaaaga cgtaggccgc accggagtcg ggcgccgcct
     4621 cattgggcgt gcggttgatg ccagtgaggc tgctgtcctc aaaactcgcg ccgaccacca
     4681 cgctgtcccc gctgaccgcc accgagatgc cgaaacggtc atcctgcccc gcattgccgg
     4741 ctttcagata ggcctgctgg ctccacgtcg tgccgctgcg cacaaacaca taggccgccc
     4801 cggcgctggt ggcggtttca ttgggggtgc tgttgatgcc cgtggtgctg ctgtcctcaa
     4861 agtaagcccc gacgaccacc gtgtccccgc tcaccgccac ggaggtgccg aagttgtcac
     4921 ccgccgtcac ctgatgggcc ttcaaatagg cctgctgggt ccaagtcgtg ccgctgcgtg
     4981 caaacacata ggccgccccg gcactgatgg cgctctcatt cggggtactg tcaatgcccg
     5041 tggtgctgct gtcctcgaaa cgtgagccga cgaccacggt atccccatcc accgccaccg
     5101 agttgccgaa gttgtcattc gcgttcactt ggtgaggctt gagataggcc tcctgcgtcc
     5161 aggtcacgcc gctgcgcacg aacacatagg ccgcgccggc attgacggcg ctctcatcag
     5221 gcgtgctgtt gactcccgtg ctgccgctgc gctcagcgac tcctgagatg gccgcagtgt
     5281 ccccgctcac cgccaccgaa aagccaaact ggtcaaactc cgagacctgg tgagccttca
     5341 ggtaggcctg ctgggtccag gtggagccgc tgcgcacaaa gacataggcc gctccggcac
     5401 tggtggcgct ttcatcgggc gtgctgttga cgccggtggt gccgctgtcc tcaaaattcg
     5461 ccccgacgac caccgtgtct ccactcaccg ccaccgagac accgaaattg tctcccacag
     5521 agacctgatg ggacttcagg taggcctgtt gcgtccaggt ggatccgctg cgcacaaaca
     5581 cataggccgc gccggtattg ccgaggccct cattgggcgt gctgttgacc ccggtggtat
     5641 tgctgtcctc aagataaccc ccgactacga cggtgtctcc gctcacggcc accgatccgc
     5701 caaagaaatc acccgatgtg acctgatggg ccttcaagta cgcctgctgg gtccaggtgg
     5761 tgccgttgcg cacaaacaca taagcggctc ccgcgttatt ggccagttca tcaggcgtgc
     5821 tgttgatgcc cgtggtgccg ctgtcttcaa atcgagcgcc aacgaccacc gtgtctccac
     5881 ttacggcgac cgattcgcca aagccgtccc ccggcccggc ggtgtcccgc ggatcggacc
     5941 gcttcatata cgcctgctgg gcgatggggt cgatggtgac ggggtagcga gcatccgcgt
     6001 cctccacgac gagacgcacc tgtcctgccc ctgccgcctc gaagcgggag gggagcgtct
     6061 tcccatccgc gtcccacacc ttcagcccgg tgtagttcag caccgtggcc ccggcgtcgt
     6121 cggtaaagtg gacgcccagc gcgtcagcgg cgatcttcgg tgagagattg ccccgcgtgg
     6181 cgagaaccag cgaaaggctg gatggcggca aggcagaggg gcgcgtggca atgacgtagc
     6241 catgctccag gccgcgctgg tcattcaccc accactcttg caccgtcgca tcccaatcgt
     6301 aggtcaggcg ctggtcctcg gccttcacct gcggcgggct ggcacccacg ggcgtctgct
     6361 tgtccccgac gccgtagctg cgcagttcca ggccccaggt ccagccgccc tcgcgcggcg
     6421 tggcgatgaa gccgcgtccg tcaaaccgcg tggtccactg ctggcccgga ttgcgcgcct
     6481 gccagtgacc gtcctggccc tcgaccggca tgaacgcatg acgccccgcc tcgtaggccg
     6541 ctcgaatgct ctgccagtcg gacgcattca ggcctgcggg cgtcttgcca gcagacggag
     6601 cagccggttc atggctggcg gcctgggcgc gggcatcagg aaccatgcac gcaatgagcg
     6661 cgatgacgaa aacagcgagc gaatggagtt tcatggcggg aagaaggggc atcgctcctt
     6721 tctttcccat gaagcttacc gaaaccttac cacgagccgg atttctccgg cttttttacc
     6781 gatgacaatg gagcgtgaac gtcgcgcctt ttcccggctc ggactgcacg gagattttgg
     6841 cgcccaacgc ctccgcgaaa cgctgggcaa tcgccagccc caatcccttc gcgccctcgg
     6901 accgtgagcg ggcacgatcc gcacggtaaa aggcatcgaa gatgtgcggg aggtccttcg
     6961 gcgcgatgcc cggcccgtcg tcaatgacgg agatgcggca gtctccaggc tccgtcctga
     7021 cttcgatccg caccgcgccc ccttccttgc agtagcgcag ggcgttggag aggaggttgt
     7081 tcaagacgat cttgagcagc tcaggatcgg tgctggctgg aagcgcccct gtggcgtcca
     7141 tttcaatcct cgcgcccttt gcccgtgcca cgtcttggaa agacagcagc acctcgccga
     7201 gcaaacccac gagatccacc tttcgcacct gcggcttgag ctcatgcacg tcggcggaga
     7261 cgaggtccag catgcggacg acgagatggc tcatctcacg cgtgttgcgc tcccacagca
     7321 ggatggcctc gcggtagtca gcttcggctt gcgctcgttt gagggtggac tgtgcggtgg
     7381 tgagcatcgc ggccagcggc gttttgagct cgtggctgag attggcgatc aattcggccc
     7441 gcgactccgc cagcgcttcc gctctcgctt tttgaaccag ggccgactgg atgaagtgca
     7501 gcaccaacac catcgcggcg gcgatgaaca ccagggcgtt gatcacatag agccagttgg
     7561 cttggaccgc ggagagctga atgaccggcg gccttgtcat caaaggtgga aaccacccaa
     7621 ggaaaaacgc catcggaaac aaggccatca gcacgcggag gcggatgggg atgtggtcaa
     7681 agatcagcgt caccacggtg agaagcagca ggtgaaaatg aaagccgcca ccccatccgt
     7741 aggccgccgt gagccccgag gagagatgcg tccactccgc gcccaggatc aggatcagcc
     7801 agttccagcg cttcacgcgc aacagcggca ggccaatggc aaaggcagcc gtgctgacgc
     7861 cgaacatccc ggcggcgaaa gcgtgcccgc cccacgccaa cgacgcgcag atcagcgcac
     7921 gaattccaaa accccacacg aagtagcggc ggaggaactt ctgcagagcc ggcgaaggct
     7981 gccgccccga cgtgatcgaa tgtccgcccg tcctccacat catgtgtctc ctcccagtcg
     8041 cggcacgaga tagccgagcc cacgtttggt ctgaatgaag tcatgaccca gcttcgagcg
     8101 gatgcggtgg atgtgcacct ccagcgagtt cgactgaaat tttccgaagt cctcatgcag
     8161 ccgctcctcc agcacctgct tcgacaccgg gcggcctttc cgtgaaagca gcagctccac
     8221 ggtggcgaac tcactggcgc ggagcaccac ctccttgccg cccttgaaca cccgtttggc
     8281 gccaaaatgg aactccagat cgccatgcgt cagagcttcg tcgctccatg ccgaggaccg
     8341 tcgcagcagc gcccggatcc gcgccagcaa ctccgccagc tcgaacggtt tccccaaata
     8401 atcgtccgcg ccgaggtcca gccccttcac acgatccgtc aaacggttga gcgccgtcag
     8461 catcagcacc ggaacctgtt tcttcttccg caggcttcgc aacacctcgg ttccatccat
     8521 gcccggcagc agccggtcca gaatgaccag gtcatgatcc cattcgatcg cacggaaaag
     8581 accgccgctg ccggtgcgct cccactccac ggcaaaaccc tctgccttca gttcccgcac
     8641 tatcgcggca ccgagatcaa ggtcgtcttc gatcaatagg atcagcatgg catgtcgaat
     8701 ctgcgctcat gctgggagcc cgaggtgaaa atgcaacccg catcacacgg atcgcccccg
     8761 acggcgcaaa atagcccgcg gttggcaagc ttttggtaag cttgggaggt cgttcccgaa
     8821 agatgaggac cgctcaagaa tcgaggaaca ccaaaccacc gctagttctt gatcttgatt
     8881 ttgccgaagt ggatgtgcgt gcgcgccttg tccggcgtgc cgctgtccca cacggcgcgg
     8941 aagtcgccgg gagccacttc gacgagcgtg ggatatgaat tcttgatgcc ggcatcgtaa
     9001 aaggtcttct ccgcgctcca tgcgccgccc attggctgca ccttgtaacg caatgatgtg
     9061 cgtccggcct tggcgggcat cgcggggccg tcgttataga cgtaaaggtg gctgccgtcg
     9121 gcattgcgag agaagtagcc tttggaacgc gcattccaga gttccggctc ctgcttcgcc
     9181 gctgtccagg tagtgccgcc atccttgctg atgctcgaaa acgcacgcgg agggtcgagc
     9241 gcgccctttt cctcccagtt cgccgtgcgc atgacgattt tgagctcgtc aggtgaatcg
     9301 cccacggcga tgccgccttc gtggaggaag actttgtcac ccgtttcagg ctgcggaatg
     9361 taaccctcga acttccattc cagcaggctg gtgctgctga gcacgaattg atcgcgcgaa
     9421 ccgagcgggt cgttgcgcag tgtgttgcga tgcgcgggga aaaggtaacg cgtcttgcct
     9481 ttaaccttcg tctcgatcgg tccggcgacg acgatcaacg gtccgctgta gtgcatcgcc
     9541 agttcgacag gcgtccacga gcgccctcca tccgcggtgt aagcggcgac gagctgcgag
     9601 tcctcgctgt ggcgatagtt catcgggcag cgcatcgcat agcaccacat gacctcctga
     9661 tcggaaggct tgaagagaat cgcgttcgcg taggcgaaat gaatcgcccc ctgccagtga
     9721 ttgtgatcga agaccggcac cgcctcgctc caggtgtcgc cgtcgttggt cgaaacgctt
     9781 gcgtagatgc tgcccatgtc cgtcttaccg ggaatgcccg cgccataggc cacgacaagg
     9841 tgcttcggct tccattgcgc aatgtagggc tcccagacga gcgaagcgcc cggatgctgg
     9901 tccatgtgac gcacgacgcg cacgtctttg acatcgccct tgtaatcatc cgcggcgatg
     9961 aggctcgcgg cagcagtgag gaagaagaga atgatgcgtt tcatggcttg gcaggttggt
    10021 tccagaggat tttggcgagc agcacgcggt tgttttcgtc tttgcgggct tcggggaagg
    10081 ccatttcgct gctgatgacc caggtttcat tcgcattcac atccacaggc gcaaagccgc
    10141 cagtgagatc gacgcccgtc tcaggcatca ggatctgctc ggtggcacga atgatgtgca
    10201 gcttctctgg atcgacctcg gcgatgaaaa tgggcgcgcg gttgcgaaaa acgtggtcgt
    10261 tgttcgcgcc tttgcggctg taggtgaggt aaagcttttc gccgtgcgtg atccagtgct
    10321 gctgggtgtt cgcgctgagc agcggctggc cgtcgtcgaa cttccattcg acagtcggct
    10381 gataatccaa tccgtcatcg ctgcgcgtga cgtggccgtt ttgctccccg cgcatggtga
    10441 ggaaatagcg gccttggaac gagcaaacgg aaggctcata caacccgcgc gggatgctga
    10501 tggtgtgctc gctgccatgc tcgacataag ccagcgtttc gccatcgaac gcacagcgcg
    10561 tgacgactga agtgtaaact cgcgtcttcg gatcagcccg atagcgaacc ggcagcagca
    10621 cggcgccatc cggcaggtcg tggcgctgat ggcagcccgc gttcggctcg atgatcggat
    10681 tccccgcatg acccttctcc ggcatggcca tgatcttcat gccgctccac acgtccgact
    10741 tgggatcata caccgcataa gccacgcgct cctgggagcg gtcgtccttg gccttgttgt
    10801 cattcggatt ggcgaggaag ccgaaacact tgccggtgac gagcagtttg ccggacttct
    10861 cgtgccactg cgggcagatg tcgcccatca cgcgctcgat gccgtcgggg aagcgcttgc
    10921 gattgagcgc ggcgatgcgc tgcggcgcgc tccaggtctt tgcgccgtcg gtcgtctccg
    10981 tgatgtaaat gtcgcgatag ccatgcgagc cgcgcttttc gatctcctgc atggttacga
    11041 tcacgcgccc gcccggcagg aaggccggcc gcgactgcgc ccagtgatac gcgccgctgc
    11101 tcgtgagcac ggtagtgttt tcgacgcgga agtcggcggc gcacagggga gcggcgacaa
    11161 aaagcaatgt ggcgaggcct ttcatgggaa agggagacgg ggccggcccg ccttgtgttt
    11221 ctggtcatcg aggatgacca cggcccgtca gatctgcggc agaccgccgc gctcattgcg
    11281 gctgccgcag gcggggcagc gggtaatggc gcggtctttg ccggggatgg cgtagttgca
    11341 gccgcagatg cggcactgga tggcgatgcg gcggcgcatg gcctcgcggc gctcctcccg
    11401 ccagaaggtg taaaaccaca gggcgcagac gaagctgacg ccgatgagca tgaccagcat
    11461 gatgagctgg ccgaggctga tggtgatcat ggcgcggcat cctcccagcg gaatgacacc
    11521 cggccctggc cggccttccc ggagctgtcg ggacggaagc gcatccgcgc caccgcctcg
    11581 cgcaggcgcg ccgtcacctc cgcgctgtcg gcggcggcca gcggcagggc ggcg
//
LOCUS       DAOMYJ010000168        11630 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Prosthecobacter sp. isolate AMR_MDS_5629
            NODE_1840_length_11630_cov_18.472916, whole genome shotgun
            sequence.
ACCESSION   DAOMYJ010000168 DAOMYJ010000000
VERSION     DAOMYJ010000168.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867850
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prosthecobacter sp. (wastewater metagenome)
  ORGANISM  Prosthecobacter sp.
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Verrucomicrobiaceae; Prosthecobacter.
REFERENCE   1  (bases 1 to 11630)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11630)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.30
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:34:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,742
            CDSs (total)                      :: 4,684
            Genes (coding)                    :: 4,676
            CDSs (with protein)               :: 4,676
            Genes (RNA)                       :: 58
            tRNAs                             :: 55
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11630
                     /organism="Prosthecobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1840_length_11630_cov_18.472916"
                     /isolate="AMR_MDS_5629"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1965333"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1055
                     /locus_tag="PLP58_15745"
     CDS             <1..1055
                     /locus_tag="PLP58_15745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009963718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="terpene cyclase/mutase family protein"
                     /protein_id="HRJ10032.1"
                     /translation="EKLRQNGGSPECERAVSASLEWLKSKQNPDGSWPGPSKSAMTGL
                     ALLCYLGRCETPDSPFYGDNVMRGIMYLIELSKKNPQGMIAEDVYSNGSTYAHGIATY
                     ALGEMYTLARLGSKELPGMREAFEKGVKLIIDSQNKRGSWTYGGKDAGKPTAYNPDSG
                     GEDLSVAGWQFQALKAAKNTSLKIPGLDAAINKCVDYVLAKQTKDGGFGNPDRDKHYN
                     QWSLTGAGSLALQTMAKGKTTAVKKSLGFLREFLTAEPLDWEKNCNLYCWYYYTQTFF
                     QAGGDDWKFYNEQFLPQILAAQQPDGSFKRGRPNWPAGDAAHEIYRQTLCTLQLEVYY
                     RYLKVGDREEQSFFDR"
     gene            1164..1973
                     /locus_tag="PLP58_15750"
     CDS             1164..1973
                     /locus_tag="PLP58_15750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009961974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HRJ10033.1"
                     /translation="MPLPLEDLFNDVVNKAQFGLGLTTEALAAAANVEPAALEAVKDG
                     AVDKAVLARVAPALQLHGPSLIALAENAWQPAPVSLEGLEQFNTAFGDMTVNAYLVYD
                     PATREAAVFDTGASAAGIVERLRALGLTLRTLFLTHTHADHVEDIATVDAPEILVSER
                     EDHPGATTFTPGATWTLGGLTIESRSTWGHSKGGTTFVVRGLARPVAIVGDAIFAGSM
                     GGGKVSYADALATNRKEIFSLPDDTIIAPGHGPMTSVGEEKARNPFYPEFK"
     gene            complement(1987..2715)
                     /locus_tag="PLP58_15755"
     CDS             complement(1987..2715)
                     /locus_tag="PLP58_15755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ10034.1"
                     /translation="MPSFAQLLCSALLAFALTGCGSLEKSGAKEEQGRTVEQSRADAS
                     PSVDLAFLLSMNYAEAKALSGQSLELGMTVKLAAENIEVLKTDADGTPRKVRAKGRVY
                     LELGFGDTAKILCQEAWLNGDEAVVRGRPILQRGGSIIEGMADDTLFYVFGTRLRVLG
                     LHRVTNQRAMLAAPGPGMPIASAWTQGPNPLLPSLDETVVPLDIRQEMQRAAEAEAVL
                     QMNRDEALQAPAGAPAPWVKDTQP"
     gene            complement(3021..3353)
                     /locus_tag="PLP58_15760"
     CDS             complement(3021..3353)
                     /locus_tag="PLP58_15760"
                     /inference="COORDINATES: protein motif:HMM:NF013964.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease signature motif containing
                     protein"
                     /protein_id="HRJ10035.1"
                     /translation="MSGQIPAALRRLVFERAAGLCEYCLIHQSDAHFTFPVDHIISRK
                     HQGATKAANLALACLRCNVTKGTDVGALIGRPRRLSRLYHPRKDDWRAHFHLAGARIV
                     PGLANAST"
     gene            complement(3350..3724)
                     /locus_tag="PLP58_15765"
     CDS             complement(3350..3724)
                     /locus_tag="PLP58_15765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ10036.1"
                     /translation="MLRAVHDTDVVLAAQRSTFESWTETFAMPPADNSHGGAYDAGVK
                     PFEEIVQFIADAAGAERLSAFRPSAAAESRVSELLAKQQEETLTPKEREELQLFVQLD
                     HVMSLAKARAQARHGTRRLMKA"
     gene            4033..5481
                     /locus_tag="PLP58_15770"
     CDS             4033..5481
                     /locus_tag="PLP58_15770"
                     /inference="COORDINATES: protein motif:HMM:NF014675.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 10 glycosylhydrolase"
                     /protein_id="HRJ10037.1"
                     /translation="MLLLHAGTAAAQTQVPQPPRELRGSWIATVHCINWPSEPGLPVP
                     RQQEQLRRLIQSAHDRGLNCLIFQARPAGDALYESAIEPWSPYLTGKMGRAPEPKWDP
                     LAFAVTEAHRHGMELHAWFNPFRALAGDKHAPGGNHIRVTHPQWTVKYGNDWWMNPGI
                     REVRERAVQVMLDVARRYDVDGIHMDDYFYPYPIAGADKKAVPFDDAAAFAAYRATGG
                     ALDLSAWRRQNVDEVVYSTHRGLRGIKPAVKFGISPFGLWRPNHPEGTGGGLDPYEDL
                     AADSRKWLQQGWVDYLTPQLYWTIDRPKLGFATFYDWWLAQNPQGRHIWPGMNSSKVG
                     DDRNAGEILRQISVLRERGARMTPGHFHWNFNALDKDLGKLGSLAKERAYNVHAIPPA
                     SPWLSNVGLPAPLVEKYEGGKKVRWAFRDPRWDTHGRWWIVQALIEGRWLTIEVTFRD
                     RRELDWPEKATALAIRAAGRAWEVGAVALLTK"
     gene            5601..6203
                     /locus_tag="PLP58_15775"
     CDS             5601..6203
                     /locus_tag="PLP58_15775"
                     /inference="COORDINATES: protein motif:HMM:NF024911.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HRJ10038.1"
                     /translation="MKPTHPHRCRIVLTGGPGGGKTTAADLFRREIGERVVLVPEAAT
                     MVFSGGFPRVHEPHAVHAAQRAIYHVQRNLEDVQAALYPDRVLLCDRGTVDGAAYWPG
                     EPAGFFTDMGSSMKAELERYDAVIFFESAAVGGMGIEGGNPTRIESLQQAVELDRKLR
                     ALWSRHPRFHLVPHNASFFKKISFGLAVLEGVVNELAAAR"
     gene            6259..6627
                     /locus_tag="PLP58_15780"
     CDS             6259..6627
                     /locus_tag="PLP58_15780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ10039.1"
                     /translation="MSDSPFPSAPDSPGTDPLQAAKASALKAADELRAAAVQKAGELR
                     QAAGERAAQLKNAAGQTAQDLRERAGEFGHAAQDTLSQARAQCEDLLAEAEKMAREKP
                     RQALLTAFGVGLIVGLILRR"
     gene            6764..7147
                     /locus_tag="PLP58_15785"
     CDS             6764..7147
                     /locus_tag="PLP58_15785"
                     /inference="COORDINATES: protein motif:HMM:NF018980.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HRJ10040.1"
                     /translation="MDIEPSRLKSLLRALALYAEARGRLLQIEAREAGAHLAAVMMAA
                     ALTAGSLLAGWLLALPALVWLLAEGISRPWWQVALAVAGLHFLAALAGAVFLALRLRR
                     MTVFDETFRQFQKDREWISGSHDPQ"
     gene            7144..7533
                     /locus_tag="PLP58_15790"
     CDS             7144..7533
                     /locus_tag="PLP58_15790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ10041.1"
                     /translation="MKTKAQILQELDSARAELGIHLRHATMEWSPAAVVRRSFERHRW
                     AWLAGGTLAGLLCVRLLSPAAQGKFRRDNSDAPVKKSGLIWQILAPVIPLARQFALKH
                     GLEILQSHLNPPPHPPRDEAPPPAPPP"
     gene            7555..8670
                     /locus_tag="PLP58_15795"
     CDS             7555..8670
                     /locus_tag="PLP58_15795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012419224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus twitching motility protein PilT"
                     /protein_id="HRJ10042.1"
                     /translation="MSDTAEQAATPVQDLVPVLGHVDDYLRFCMQYDASDLHLATASQ
                     PIWRRYGNLTPIWNNSAELSPEDTRRLAYSFLTENQIQQLETKGDVDFAYSPEFGRFR
                     ASVVQQRLGIDITFRIITTNIRTVDELGLPDTVRTLIRYHNGLVLVTGPVGCGKSTTL
                     AALVDEINKERQDHIITLEDPIEYVLEPKGCHVNQREVGSHTESFAAALRGALREDPD
                     VIMVGEMRDLETISLAITAAETGHLVLGTLHTGNSARTLDRVLDVFPSDQRDQIRIMV
                     SESLRGIISQQLVPRTDGNGRAMAMELLVNTPAVAACIRDGKTYLLNGVMQTGKNVGM
                     ITMDDSLRGLYSKGLISREECESRAEDKQLMRAFFKS"
     gene            8737..9843
                     /locus_tag="PLP58_15800"
     CDS             8737..9843
                     /locus_tag="PLP58_15800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012419225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus twitching motility protein PilT"
                     /protein_id="HRJ10043.1"
                     /translation="MPIIDQLFTQLIELGGSDLHLSQGQPAKIRVHGSIKPISEDVLT
                     SDTMETMMREICEPRAWERYVERGDLDFAYEMDADHRFRCNYLKQQNGYACVFRIIPT
                     KIASLESLGIPPVVKEFGHLRSGLVLVTGPTGSGKSTTLAALLDYINVNFRRHIITIE
                     EPIEFVHRNKRSIITQREVPIQTPSFADGLRAALRQDADICLVGEMRDLETISLALTA
                     AETGLLVFGTLHTNNARKTVDRIIDVFPSDQQSQVRTMLAASLKGVVAQLLMKKADGK
                     GRVAVNEVMVSTPAVGAIIREGATQKLYDVIIGGKAEGMQFMDDSIWSKLRDGYVSPM
                     EAYMKAIDKNRFKAFLPPEMQAVANAGGGTENKK"
     gene            10166..10474
                     /locus_tag="PLP58_15805"
     CDS             10166..10474
                     /locus_tag="PLP58_15805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ10044.1"
                     /translation="MNPTNQRGSWALVVIPLALNALLLAVSLPLLALPEGARIRPVGW
                     AFAILLITLMGWGTGLVALDEMRRRRSWAKPLPALLLCLLPLPLAAGILHFKGLVLAH
                     "
     assembly_gap    11354..11453
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         2059 a         4048 c         3415 g         2008 t
ORIGIN      
        1 tggagaagct gcgccagaat ggcggctcgc cggagtgcga gcgcgcggtg tccgcatcgc
       61 ttgaatggct gaaatccaaa caaaacccag acggttcctg gccggggccg agcaagtcgg
      121 caatgacggg cctggccctg ctttgctacc tgggccggtg cgagacgcca gactcgccct
      181 tctacgggga caatgtcatg cgcggcatca tgtatctgat cgaactgtcc aagaagaacc
      241 cgcagggcat gatcgccgag gatgtttaca gcaacggctc cacctacgca cacggcatcg
      301 ccacttacgc tctgggggag atgtacacgc tggcgcgcct cggcagcaag gagctgcccg
      361 gcatgaggga ggccttcgaa aaaggcgtca agctcatcat agacagccag aacaagcgcg
      421 gctcctggac ctacggcggc aaggacgccg gcaagcccac cgcctacaat cccgacagcg
      481 gcggcgagga tctttccgtg gccggatggc agttccaggc gctcaaagcc gccaaaaaca
      541 ccagcctcaa aatccccggt ctcgacgccg ccatcaacaa atgcgtggac tatgtgctgg
      601 ccaagcagac caaggacggc ggcttcggca atcccgaccg cgacaagcac tacaatcaat
      661 ggagccttac cggcgccgga tcactggccc tccagaccat ggccaagggc aagaccacgg
      721 cggtgaaaaa gagcctcggc tttctgcgcg agttcctcac cgccgagccg ctcgactggg
      781 agaagaattg caatctctac tgctggtact actacaccca gacctttttc caggccggcg
      841 gtgacgactg gaagttttac aacgagcaat tcctcccgca gatcctggcc gcgcagcagc
      901 ccgacggcag cttcaagcgc ggccggccca actggcctgc gggcgacgcc gcccatgaga
      961 tctaccggca gaccctctgc accctccaac tcgaagtcta ttaccgctat ctgaaagtcg
     1021 gcgaccgcga ggagcagagc ttcttcgacc gttgagagcg cgcggctgct catccagagt
     1081 catcacgaaa atcggaattc gaatttctga tttcgaattt cgatctctct ttccctcccc
     1141 ccttcatagt ccatcaagcc cccatgcccc tgccactcga agaccttttc aatgatgtcg
     1201 tcaacaaagc ccaattcggc cttggcctga ccacggaggc gctggccgcc gccgccaatg
     1261 tcgaacctgc cgcgctggag gcggtgaagg acggcgcggt ggacaaggcg gtgctcgcgc
     1321 gcgtggcgcc ggcgttgcag cttcacgggc cttctctcat cgctctcgcg gaaaatgcct
     1381 ggcagcccgc gcccgtgagc ctggaggggc tggagcagtt caacacggcc tttggcgaca
     1441 tgaccgtgaa cgcctacctc gtttatgacc cggccacgcg cgaggcggct gtctttgaca
     1501 cgggtgcctc ggcggccggc atcgtggaaa ggctgcgcgc cctcggcctc actctgcgca
     1561 cgcttttcct cacgcacacg cacgcggatc atgtggagga cattgccacg gtggacgcgc
     1621 ctgaaatcct cgtcagcgag cgcgaggatc acccgggtgc cacgacattc actcctggcg
     1681 ccacatggac tctcggcggg ctgaccatcg agtcgcgttc gacttggggg cactcgaagg
     1741 gcggcaccac ctttgtcgtg cgcgggctgg cgcggccggt ggcgattgtg ggtgacgcga
     1801 tttttgccgg ctccatgggc ggcggcaagg tctcttatgc ggatgctctc gccacgaatc
     1861 ggaaggaaat tttttccctt cctgatgaca ccatcatcgc cccgggccac ggtcccatga
     1921 ccagcgtcgg cgaggaaaag gcgcgcaatc ctttttaccc cgagttcaaa taagccgccc
     1981 gcaggctcag ggctgcgtgt ctttgaccca gggcgcgggc gctcccgccg gtgcttgcag
     2041 cgcctcgtcg cggttcattt gcagcacggc ctccgcctcg gcggcgcgct gcatttcctg
     2101 gcggatgtcg agcggcacca cggtctcgtc gagagagggc aggagcggat tcggaccctg
     2161 tgtccaggcg ctggcgattg gcatgcccgg ccccggtgcg gcgagcatgg cgcgctggtt
     2221 ggtcacgcgg tgcaggccca gcacgcgcag ccgggtgccg aagacataga agagcgtgtc
     2281 atccgccatg ccctcgatga tgctgccgcc gcgctgcaga atgggccgtc cgcgcaccac
     2341 ggcctcgtcg ccgttcagcc aggcctcctg gcagaggatc ttcgccgtgt cgccgaagcc
     2401 gagctcgagg tacaccctgc ccttggcgcg caccttgcgc ggggtgccgt cggcgtcggt
     2461 cttcagcacc tcgatgtttt ccgccgccag cttgaccgtc attcccagct ccaggctttg
     2521 cccgctcagc gccttggcct ccgcgtagtt catgctcagc aggaaagcca ggtccaccga
     2581 gggtgaggcg tccgcccggc tttgctccac cgtcctgcct tgctcctcct tcgccccgct
     2641 tttttccaaa gacccgcaac ccgtcaaggc gaacgccagc agggcggagc agagaagttg
     2701 ggcaaacgag ggcatcgggc tttcgaaaag gatacgccca tagaacaccc ctccggcgcg
     2761 cgcatcaagc cgcgaaggtt acatgccgcg cgcagccggt tctctgcgcc gggagaagtg
     2821 agggcttggg gccggcgttg ttcttttcaa ccacgcaggt aggccagggc ctccagagag
     2881 gggtagcgcc cggccctggc cagcgcgcgc cgaagcatca ggcggtcgcg cgcgttcaag
     2941 tcgagcaaac gcaccgtcgc ctccccaaca tccgacactg gcacaatgcg cgcgcctgcg
     3001 agatggaaga cctcggcggc ctacgtcgag gcattcgcca agcctggcac aatgcgcgcg
     3061 cctgcgagat ggaagtgagc ccgccaatca tccttgcgag ggtggtagag acgcgacagt
     3121 cgtctcggcc ggccgatcag cgcgcccaca tccgtgcctt tcgtcacatt gcagcgcagg
     3181 caggctaacg ccagattcgc cgccttggtg gcaccttgat gtttgcggct gatgatgtga
     3241 tccaccggaa aggtgaaatg cgcgtcgctt tgatggatga ggcagtattc acacaagccg
     3301 gctgcgcgct caaagaccag acggcgcagc gcggctggaa tttggccgct catgctttca
     3361 tcaagcggcg ggttccatgg cgggcctgcg cgcgcgcctt ggcaaggctc atcacatgat
     3421 caagctgcac gaagagttgc agttcctcgc gctcctttgg cgtcaatgtc tcttcctgtt
     3481 gtttggcgag cagttcggac acccggcttt ccgccgcggc tgagggccgg aaagcgctca
     3541 agcgctccgc tccggccgca tccgcgatga attggacgat ttcttcaaaa ggtttcacgc
     3601 ccgcatcata agcccctccg tgcgagttgt cagccggcgg catggcaaat gtttctgtcc
     3661 aagactcgaa agtgctccgt tgtgctgcga gtacgacatc ggtgtcatga acggctctga
     3721 gcatggtgcg cgggttatcc cttcatcgct gtggtgacac tttcatgcag gtcgaggaaa
     3781 ccgccggcgc ctcttcgcgg ggcaccattt ccacaaggct cgtggattca gcggccagct
     3841 cccgcgcttt atcggaatat cttcgggaac gccaatgttc caagtccacc tgctggcgcg
     3901 cgaagagcag caaacggttc ctagtgtggg gctgataccg tcacgtccag tcctctgcgc
     3961 gcagtccgcg aaggttgaca tgccgcgcgc cgccggttct ctgcgcgcat gacgcgccgg
     4021 cttttattct ccatgcttct gctgcacgct ggcaccgccg ccgcgcagac gcaggtgccg
     4081 cagcctccgc gcgagctgcg cggctcgtgg atcgccaccg tgcattgcat caactggccc
     4141 tccgagcccg gcctgcccgt gccgcgccag caggagcagc ttcgtcgcct cattcagtcc
     4201 gcgcatgatc gaggcctgaa ctgcctcatt ttccaggcgc gtcccgccgg agacgcgctc
     4261 tatgagtcgg ccatcgagcc atggtcgccc tacctcacgg gaaaaatggg ccgcgctccc
     4321 gagccgaagt gggacccgct ggccttcgcc gtcaccgagg cgcaccggca cggcatggag
     4381 ctgcacgcct ggttcaaccc cttccgcgcc ctggccggcg acaagcacgc gccgggcggc
     4441 aaccacatcc gcgtcacgca tccgcagtgg acggtgaaat acgggaatga ttggtggatg
     4501 aaccccggca tccgcgaggt gcgcgagcgc gccgtgcagg tcatgctcga tgtggcccgc
     4561 cgctacgatg tggatggcat ccacatggac gattattttt atccataccc catcgccggc
     4621 gcggacaaaa aagccgtgcc cttcgatgac gcggccgcct tcgccgccta ccgtgccacg
     4681 ggtggcgcgc tcgatctcag cgcctggcgg cggcagaatg tggacgaggt ggtttactcc
     4741 acccaccgcg gactgcgcgg catcaagccg gcggtgaagt ttggcatcag ccccttcggc
     4801 ctgtggcggc cgaaccatcc cgagggcacc ggcggcgggc tcgacccgta tgaggacctg
     4861 gccgccgaca gccgcaagtg gctccagcaa ggctgggtgg actacctcac accgcagctt
     4921 tactggacca ttgaccggcc caagctcggc ttcgccactt tctacgactg gtggctggcg
     4981 cagaatcccc agggccggca catctggcct ggcatgaaca gcagcaaggt tggcgacgac
     5041 cgcaacgcgg gcgagatcct ccgccagatc agcgtgctgc gcgagcgcgg cgcgcgcatg
     5101 acgccggggc acttccactg gaacttcaac gcgctcgaca aagacctcgg caagctcggc
     5161 tccctggcca aagagcgcgc ctacaacgtc cacgccatcc cgcccgccag cccctggctc
     5221 agcaatgtgg ggctgccagc gccgctggtg gagaaatacg agggcggcaa aaaagtccgc
     5281 tgggctttca gagacccgcg ctgggacact catggccgct ggtggatcgt ccaggcgctc
     5341 atcgagggcc gctggctcac cattgaggtc acatttcgag atcgtcgtga actcgactgg
     5401 ccggaaaaag ccaccgccct tgccatccgc gccgccggtc gcgcgtggga ggttggtgcg
     5461 gtggcgttgc tcaccaaatg acggcgcgct ctcccgcttt ctcctcgtaa tccgtgtctt
     5521 aatccctgtc ttaatcccgg acacagacag aaacggatta cgacaaggat taagacacgg
     5581 attacgatgg aggagacacc atgaaaccca cccacccgca ccgctgccgc atcgtcctca
     5641 ccggaggtcc cggcggtggc aaaacgaccg ccgccgacct cttccgccgc gagatcggcg
     5701 agcgcgtcgt gctcgtgccc gaggccgcca ccatggtctt cagcggcggc tttccgcgcg
     5761 tgcatgagcc tcatgccgtc catgccgcgc agcgcgccat ttatcatgtg cagcggaacc
     5821 tggaggatgt gcaggccgcg ctttaccctg accgcgtcct gctctgcgac cgcggcaccg
     5881 tggacggcgc cgcctactgg ccgggcgagc cggcgggctt tttcaccgac atgggctcca
     5941 gcatgaaggc cgagctggag cgctacgatg ccgtcatctt tttcgagagc gccgccgtcg
     6001 gcggcatggg catcgagggc gggaacccca cccgcatcga gagcctccag caggccgtgg
     6061 agctggaccg aaagctgcgc gcgctctgga gccggcaccc gcgcttccac ctcgtgccgc
     6121 acaacgcctc cttcttcaaa aaaatctcct tcggcctcgc cgtgctcgaa ggcgtggtca
     6181 acgagctcgc cgccgcccgt tgaagccgcc gctttttttg tcgccatcag tggcggatgg
     6241 ttttaaatca ccgctgtcat gagcgactca ccttttccct ccgccccgga ttcccccggc
     6301 accgatcccc tccaggccgc caaggccagc gccctgaaag ccgccgacga actgcgcgcc
     6361 gccgccgtgc aaaaagccgg cgagctgcgc caggccgcag gggagcgcgc cgcccagctc
     6421 aaaaacgccg ccggccagac cgcgcaggac ctgcgtgaaa gggccggcga gttcggccac
     6481 gccgcgcagg acaccctcag ccaggcccgc gcgcaatgcg aggacctgct cgccgaggcg
     6541 gagaaaatgg cgcgtgaaaa gccccgccag gccctgctca ccgccttcgg agtcggcctc
     6601 atcgtcggcc tcatcctgcg ccgttgaagc ccgtcggcgg agtcgttgga gttcaagctt
     6661 cagcttgtcc gcacagcccc cgcatcgaag cacctcccgt ggcgcctgaa ggcttcactc
     6721 cagcgttgag aaccgcgcgt tgaaaaaaaa tcccgtcccg ctcatggaca tcgaaccctc
     6781 caggctgaaa tccctcctgc gcgcgctggc gctttacgcc gaggcgcgcg ggcggctcct
     6841 ccagatcgag gcgcgcgagg ccggcgcaca cctggccgcc gtcatgatgg ctgcggccct
     6901 caccgcaggc agcctgctcg ccggctggtt gctggccctg cccgcgctcg tctggctgct
     6961 cgcggagggg atctcccgcc cgtggtggca ggtggctctc gctgtggccg ggctgcattt
     7021 tctggccgcc ctcgcaggcg cggtcttcct cgccctgcgc ctgcgccgca tgacggtttt
     7081 tgacgagacc ttccgccagt tccagaaaga ccgcgagtgg atcagcggca gccacgaccc
     7141 gcagtgaaga ccaaagccca aatcctccag gagctcgact ccgcccgcgc cgagctcggc
     7201 atccacctcc gccacgccac catggagtgg agccccgccg ccgtcgtgcg ccgcagcttc
     7261 gagcgccacc gctgggcctg gctggccggc ggcaccctgg cgggcctgct ttgcgtgcgt
     7321 cttctcagcc ccgccgcgca gggcaagttt aggcgtgaca actcggacgc acccgttaaa
     7381 aaaagcggtt taatttggca aattctcgcg cccgtcattc cgctcgcgcg ccagttcgcc
     7441 ctcaagcatg gcctcgaaat tctccagtcc cacctgaatc ctccccctca tccgccgcgt
     7501 gacgaggcgc cgcctcccgc gcccccgccc tgacccctct ccccagccca cctcatgtcc
     7561 gacacagccg aacaagccgc cacccccgtc caagacctcg tgcccgtcct gggccatgtg
     7621 gatgactacc tgcgcttctg catgcagtat gacgcgtcag acctccacct cgccaccgcc
     7681 tcccagccca tctggcggcg ttacggaaac ctcaccccca tctggaacaa ctccgccgag
     7741 ctcagccccg aggacacccg ccgcctcgcc tacagcttcc tgacggaaaa ccagatccag
     7801 cagctcgaaa ccaagggcga cgtggacttc gcctactccc ccgagttcgg ccgcttccgc
     7861 gcctccgtcg tccagcagcg cctcggcata gacatcacct tccgcatcat caccaccaac
     7921 atccgcaccg tggacgagct cggcctgccc gacaccgtgc gcaccctcat ccgctaccac
     7981 aacggcctcg tcctcgtcac cggccccgtc ggctgcggca agtccaccac cctcgccgcc
     8041 ctcgtggacg agatcaacaa agagcgccag gaccacatca tcaccctgga ggaccccatc
     8101 gagtatgtgc ttgagcccaa aggctgccat gtgaaccagc gcgaggtcgg cagccacacc
     8161 gagtcatttg ccgccgccct gcgcggcgcc ctgcgcgagg atccggacgt catcatggtc
     8221 ggtgaaatgc gcgacctgga gaccatctcc ctcgccatca ccgccgccga gaccggccac
     8281 ttggtcctcg gcaccctcca caccgggaac tccgcccgca ccctggatcg cgtgctcgac
     8341 gtcttcccct ccgaccagcg cgaccagatc cgcatcatgg tctccgagtc cctgcgcggc
     8401 atcatctccc agcagctcgt cccccgcacc gacggcaacg gccgcgccat ggccatggag
     8461 ctgctcgtga acacacccgc cgtcgccgcc tgcatccgcg acggcaagac ctacctcctc
     8521 aacggcgtca tgcagaccgg caaaaacgtc ggcatgatca ccatggacga ctccctgcgc
     8581 ggcctctact ccaagggcct catctcccgc gaggagtgcg agtcccgcgc cgaggacaag
     8641 cagctcatgc gcgccttctt caaaagctga ccgccttcgc aaccgccccg caccctttta
     8701 ccgcaacacc agccaggcac caccccgccc acgcccatgc ccatcattga ccaactcttc
     8761 acccagctca tcgaactcgg cggctccgac ctccacctca gccagggcca gcccgccaaa
     8821 atccgcgtcc acggctccat caagcccatc tccgaggacg tcctcacctc cgacaccatg
     8881 gaaaccatga tgcgcgagat ctgcgagcct cgcgcctggg agcgctacgt cgagcgcggc
     8941 gacctcgact tcgcctacga gatggacgcc gaccaccgct tccgctgcaa ctacctcaag
     9001 cagcaaaacg gctacgcctg cgtcttccgc atcatcccca ccaagatcgc cagcctcgaa
     9061 agcctcggca tcccccccgt cgtcaaggaa ttcggccacc tgcgcagcgg cctcgtcctc
     9121 gtcaccggcc ccaccggttc cggcaagtcc accacgctgg ccgccctcct cgactacatc
     9181 aacgtcaact tccgccgcca catcatcacc atcgaggagc ccatcgaatt cgtccaccgc
     9241 aacaagcgca gcatcatcac ccagcgcgag gtccccatcc agaccccctc cttcgccgac
     9301 ggcctgcgcg ccgccctccg ccaggacgcg gacatctgcc tcgtcggtga aatgcgcgac
     9361 ctggagacca tctccctcgc cctcaccgcc gccgagaccg gcctgctcgt cttcggcacc
     9421 ctgcacacca acaacgcccg caaaaccgtg gaccgcatca ttgacgtctt cccctccgac
     9481 cagcagagcc aggtgcgcac catgctcgcc gcctccctca aaggcgtcgt cgcccagctc
     9541 ctcatgaaaa aagccgacgg caaaggccgc gtcgccgtca acgaagtcat ggtctccacc
     9601 cccgccgtcg gcgccatcat ccgcgaaggc gccacccaga agctctacga tgtcatcatc
     9661 ggcggcaaag ccgagggcat gcagttcatg gacgactcca tctggagcaa gctgcgcgac
     9721 ggctacgtct cccccatgga ggcctacatg aaggccattg acaaaaaccg cttcaaagcc
     9781 ttcctccccc ccgaaatgca ggccgtcgcc aacgccggcg gcggcaccga gaacaagaag
     9841 tgagggggca agttatcact ctttgatcaa taaaccgcaa aatacgttat tatggctcaa
     9901 tgtcctgatt gtgatagcgc ggaaattctt tccattccaa caagtggcga tggtaagtgt
     9961 gatcaatgtc acggaaccgg agtttggaac tctcctatcg tcggtactat cgccgcaacg
    10021 atttctgggg aagatgatga atgttttaaa tgtaacggaa ctggtcaatg tcagacatgt
    10081 ggaggtacgg gcttggttga cggggatgaa tagccaggtg gagctggcgc ggtatttaac
    10141 ataaattaca cgcgatttcc tctgtttgaa cccgaccaac cagcgcggct catgggcact
    10201 cgtggtcata ccactggccc tgaacgcgct gctgctggct gtctccctcc cgctgctggc
    10261 gcttcccgaa ggcgcgcgga tacggcccgt gggctgggcc tttgccatcc tgctcatcac
    10321 cctcatggga tgggggacgg gtctcgtggc gctggatgaa atgcgccgcc gccggagttg
    10381 ggcgaagccg ctcccggccc tcctcctgtg cctgctgccg ctgcccctgg cggcgggcat
    10441 tctgcatttc aaagggctcg tgctggcgca ttaacccacg cagaaaaggc gcgcgccgcg
    10501 tccagcttcg catagtgtgc ccccaagccc aacgggcttg cgtatcatag cgaagggttg
    10561 cccgcgagcg ggctaccctg gaactgacgc ccccgtcacg aaaagccccg cgaaccctga
    10621 aagggttccg cactggcctg gcacacggcg cgcctcatcc ccaaacccgg tgcggaaccg
    10681 cgttgcggtt cgtccttgaa cacgcgggtg tgacctgcgg attccagggt agcggcttcg
    10741 ccgcaaccct tcgctgtgat gcggaaggcc cttggccttc atggggcgga agataacttg
    10801 tggatgatgg atgccacgcc cacgccgggt tgtggaagga tgctggcctt cacaagcaaa
    10861 gccaggttca ggagggttga acccattttt gtgggagaca tgtttcgggg cgggctggac
    10921 gagaatcatg cggctggtgg ggagtctccc ttgccacgat tgcggagtcg cgcagcttgc
    10981 ggggaggctt ccctgccacg attgcggagt cgcgtcgctt gcggggaggc ttccctgcca
    11041 cgattgcgga gtcgcgtcgc ttgcggggag tctcccgtgg cacgattccg gagtcgtgcc
    11101 gcttgttggg agtctcccct ggcacgattc cggagtcgcg ccgcttgttg ggagtctccc
    11161 ctgccacgat tgcggagtcg tgcggctagt tgggagtctc ccctgccacg attccggagt
    11221 cgtgccgctt gtggggaggc tcccctggca cgattccgga gtcgcgccgc ttgtggggag
    11281 tctcccgtgg cacgattgcg gagtcgtgcc gcttgtgggg aggctcccct ggcacgattc
    11341 cggagtcgcg ccgnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    11401 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnncttgtgg
    11461 ggagtctccc gtggcacgat tccggagtcg tgcggcaggt ggggagtctc cttggggggc
    11521 ggaaaaggct tttgggctgc ctccagagct gactttggcc cggtggagga gttatcccga
    11581 acgccgcttt tgaaatgaga tttgttaggg gttgaggggt cagatcgcgc
//
LOCUS       DAOMYJ010000169        11570 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Prosthecobacter sp. isolate AMR_MDS_5629
            NODE_1852_length_11570_cov_18.357620, whole genome shotgun
            sequence.
ACCESSION   DAOMYJ010000169 DAOMYJ010000000
VERSION     DAOMYJ010000169.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867850
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prosthecobacter sp. (wastewater metagenome)
  ORGANISM  Prosthecobacter sp.
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Verrucomicrobiaceae; Prosthecobacter.
REFERENCE   1  (bases 1 to 11570)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11570)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.30
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:34:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,742
            CDSs (total)                      :: 4,684
            Genes (coding)                    :: 4,676
            CDSs (with protein)               :: 4,676
            Genes (RNA)                       :: 58
            tRNAs                             :: 55
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11570
                     /organism="Prosthecobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1852_length_11570_cov_18.357620"
                     /isolate="AMR_MDS_5629"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1965333"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2033)
                     /locus_tag="PLP58_15810"
     CDS             complement(<1..2033)
                     /locus_tag="PLP58_15810"
                     /inference="COORDINATES: protein motif:HMM:NF012403.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140658 - ATP-dependent chromatin
                     remodeler activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SNF2-related protein"
                     /protein_id="HRJ10045.1"
                     /translation="MPHPALSPEGILHCEGAPEDSTGRKLAAAAVEGEAALLLLLAAG
                     LLDAELDAPWRWLREWPRRFLTRLCQTRDPAAVTPPSVDETAAFLAAAPPFPGSEHLT
                     SETALRWWQALTGHVTALIAAHPGGVGDWLRLENPAWHAVGRVTFHLAENKTDTQRPF
                     AFLATFTEKLNASGQPQHLPLARALQLYAGQKDQAALAALLEPVRTAAARSRLVREWL
                     ETRRLFQPLAMTPPEAFRLLKESPALQESGIVMKLPDWWRGGKGPRPAVSVTVDAPKK
                     TSLHAGALLSFKISQSLDGEPLTEAEWEKLLSAETGLVSLRGRWVEVDGDQLRQVLAH
                     WRKVRHAAGEGGIGFLDGMRLLAGFDPRAAAAGGDESAAQVPDWSQVIAGKALARALE
                     EIRAPGEMPPPPGLRATLRPYQQKGFAWLHGMTRLGLGACLADDMGLGKTLQVIALLL
                     SRRRACQSVENAPDSSHASRVLANAPTADGDPALLVVPASLIGNWKAELARFAPELRV
                     LIAHPSQLPREQLDHAAKDPAAALRGCDAMLTTYQFLQRTESWLSHPWSLVILDEAQA
                     VKNPGSGAAKAVKKLAAPARVALTGTPVENRPGDLWSLFDFLNPGLLGPAAAFAQAVK
                     RGAGSPEGYAPLRRLVQSYILRRMKTDRSIITDLPDKIELKAFCTLTRRQATL"
     gene            2206..2406
                     /locus_tag="PLP58_15815"
     CDS             2206..2406
                     /locus_tag="PLP58_15815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="YHS domain-containing protein"
                     /protein_id="HRJ10046.1"
                     /translation="MKTLFSLLAVAGFASAAPVNTECPVAGKPAKPSVTTTHEGKEIA
                     FCCNNCKGKFEADPAKYAKKIK"
     gene            2796..3917
                     /locus_tag="PLP58_15820"
     CDS             2796..3917
                     /locus_tag="PLP58_15820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007416949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HRJ10047.1"
                     /translation="MKKVGIGIIGGGLMGREMASAFGRWCALTDVTVQPELVAVADLV
                     EPVREWFRQVPSCAQITADYHELLANPSVDVVYVAVPHNLHEKIYTDVLAAGKDLFAE
                     KPFGMDLASARNILAACRSSGRFVRCSSEFPFFPGAQRVIAEVGSGRLGRVLEVVSGF
                     HHSSDLDATKPANWKRFSRACGEIGVLGDLGMHACHIPLRLGWRPKQVFAQLQKGYPE
                     RPDGKGGMAACDTWDNALLHCWTDIGGHEAPLRLEMKRLAPGETNTWFIEVLGTEGGA
                     RYSTKEPKTLWIFEGGKVQYWKKTDLGFGMPFKAVTGGIFEPGFPDVIQQMWAAFLME
                     REGKLDGRFGCATPEEAVATQEIFAAALESQNKRAVVAV"
     gene            3981..4601
                     /gene="trmB"
                     /locus_tag="PLP58_15825"
     CDS             3981..4601
                     /gene="trmB"
                     /locus_tag="PLP58_15825"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009965976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008176 - tRNA
                     (guanine-N7-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HRJ10048.1"
                     /translation="MIHPRNLPAMSALFIPPDYFRELRREEVCADAARPLEVELGCGD
                     GTFLAGMAARHPEREFLGIERMLGRVGKTMRKIESGRLANARVMRLESGYAAGWLLPA
                     AAIARVHLLCPDPWPKKRHTARRLVNQEEFLNGLARILEPGGEFLLKTDDPPYFEDAL
                     ASLGARREFERLEWPEDAFFYPVTDFESHWLALGKEIHRARWRRMR"
     gene            complement(4653..5636)
                     /locus_tag="PLP58_15830"
     CDS             complement(4653..5636)
                     /locus_tag="PLP58_15830"
                     /inference="COORDINATES: protein motif:HMM:NF025465.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PmoA family protein"
                     /protein_id="HRJ10049.1"
                     /translation="MKLHHVLAALLVTASAQAENFTLEKTAAGGAIVKLDGQFFTEFV
                     VDQANKTYLWPVIGPDGTDMTRSYPMKTVEGEQHDHPHHRGLCFGHENIGGYDTWAER
                     STFGEKPSDKTSARVSHLGAIKHRSITEMKDGEKAVLAVVADHVDAAGKKIIEEIRRH
                     TFMVKNGARIIDVDIELIATEGDTLVDDKKDAGLSIRVPSEMAVDKGKEGKGTGRIIN
                     SEGHTDGDAWAKRATWVDYHGTVKGASVGVAMLNHPSSFRHPTPWHVRTYGLFTANPF
                     GLQSLDKNSETGAFTLKKGEKITLRHRFIFHTGDEKAAKIAEAYADYAKEP"
     gene            5701..7767
                     /locus_tag="PLP58_15835"
     CDS             5701..7767
                     /locus_tag="PLP58_15835"
                     /inference="COORDINATES: protein motif:HMM:NF012546.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HRJ10050.1"
                     /translation="MKHLFLPCLLLCLPAFEAAAQLRPDGATASFRGSLPPKPVAALS
                     AGQEAELEKELAALTAEFEPVKKHPRAADAHIFLKAVRYALDFDEWYDKTPEDGLKKA
                     RKLLAEAGGRIAALKKNRAPWMDGPGWKVLGFYSRIDDSPQPYAVEVPEGLDTGKKKA
                     PMWIWLHGRGDTATDLHFIAGRLGSAKPGQFQPQGTIVVHPFGRYCNGWKSAGETDVF
                     EVRDDAAERFGTDPDRVALAGFSMGGAGAWHIGAHYPDQWACVHAGAGFVDVRRYQKL
                     TPEKYPAWHEQKLWGMYDVPDAARNLLNVPLVVYSGEEDTQRDAAAYMEEVLAKEGLV
                     MRHHIGPGMGHKYHSEVIKTVQAEIEAAVSKGRDRFPNEVRVQARTPFYGRVKWLNLH
                     GMERSWEEARIDARVAGGRAIEIQTRNVNRIVMYPPPQARAGGDKLDVIIDGEKLTVS
                     LGPVLPEFETFMRPGPRSPAMTAAFVRLVKKDGQWTDFGGAQSFQHTGPEGGKTTAHP
                     GPMDSAFMKRFLVVLPDGRSSHAGVDAWVQAESARFIQRWRSLMRGDPRVIRADDLAD
                     PVEACKTQGLILWGTPESNSVIRKTAARLPLNWGAEKIEMAGQSHDAATHVPLMIYPA
                     LESPGFEVVINSGLTFREAHDRTNSLQNPKLPDWTILDITQPPDAEKAGRVVAADFFD
                     DFWRVK"
     gene            complement(7784..8569)
                     /gene="proB"
                     /locus_tag="PLP58_15840"
     CDS             complement(7784..8569)
                     /gene="proB"
                     /locus_tag="PLP58_15840"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: protein motif:HMM:TIGR01027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004349 - glutamate 5-kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HRJ10051.1"
                     /translation="MSPTGSKRIVIKFGSGILTRVSMPEPDPVQLRKLVEAVALLKQA
                     GHIVVVVSSGAVASGLKPMGMAARPNDMTTLQALAAVGQTHLMHAYEGLLRDHGLHVA
                     QLLVTHEDLEAAQRGARFKATLERLLEFPQVVPVINENDSVAIEELRFGDNDMLSSHI
                     ARLWGADVLLLLTSAPGLLQDPDHPEAGPIPLVEDVDAVIHHAQEKKTSLGTGGMISK
                     LKAVQDAVNAGVECVIASGRHAEQIPAVIAGTGVCTRFTAAVH"
     gene            complement(8654..9130)
                     /locus_tag="PLP58_15845"
     CDS             complement(8654..9130)
                     /locus_tag="PLP58_15845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ10052.1"
                     /translation="MKNLLALIIFASAVAGWYFYDQFKKMKAGLDEAVKNIEAYEGTV
                     AGRRAEMQAIIGALELQKKVEFRKAEVAALKTKADQARAETVNLGREKAAAVIEARQK
                     QVGRVFTEFVLADGRKLLNVRVTKVDNTGVAVTSASGVTKLRPSELTPEMRALFFY"
     gene            complement(9164..9523)
                     /locus_tag="PLP58_15850"
     CDS             complement(9164..9523)
                     /locus_tag="PLP58_15850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ10053.1"
                     /translation="MKDLRPIQAVLSGILCALMLSSCKEAEQVQADHAAAMEKLAALR
                     AEYDGLEQKLVDLRKAVPPSSSPMDSAQRMTLSLQRELDILDQQIVTMTRDYEEAQAA
                     LDKLRAETEEVKKHLQN"
     gene            9726..10280
                     /locus_tag="PLP58_15855"
     CDS             9726..10280
                     /locus_tag="PLP58_15855"
                     /inference="COORDINATES: protein motif:HMM:NF025597.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4339 domain-containing protein"
                     /protein_id="HRJ10054.1"
                     /translation="MSASYHISRGEKQLGPCSLDDLRTMLAYGSVKKTDMVRRGDAGD
                     WLPLDRLEELQQEESEDSADPRQRRRTVRYREYHKVPQDSRAGVVLKRLALGFIFFPP
                     MLWRGAVTVFQSRVVTRRTNENGYLEVWPRWVEGAVSAMIVVNALGWWVMLSWAGREA
                     APMVRAIAGTMSVGFDVLREWLGS"
     gene            complement(10287..>11570)
                     /locus_tag="PLP58_15860"
     CDS             complement(10287..>11570)
                     /locus_tag="PLP58_15860"
                     /inference="COORDINATES: protein motif:HMM:NF024938.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase family protein"
                     /protein_id="HRJ10055.1"
                     /translation="GVLRVDSIAELFQTAEVLPCRRPPRGRRLAIVTNAGGPGALAVD
                     MLARHGGAPADLGAETLARLDAFLPGPWSHSNPADILGDADAARYARALEIVAEDAGN
                     DALLVILTPQAMTDNAAVAEAVVEVAKKSGKPLLASWMGGDGVEAGRRILARAGIPCF
                     NYPDTAARVFARVTEHLSQPPVSGTCAGEEPVPNLALRVIARAEAGGRVLLNEAESKQ
                     LLGAFGIPVVQTLSADSEDEAVDHARALGFPVVLKLCSRTLTHKTDAGGVRLALTGED
                     AVRDAWREIRDAVAARAGAEHFGGVTVQRMIADKGVELILGASRDAQFGPVALFGAGG
                     VMVHVLQDTALDLAPLDVDQALRLIARTRVHRALLGARGSAPLDMGALARLVALFSRI
                     PLALPQIAEMEINPLLAAQDGFTALDARIVLRRNL"
BASE COUNT         1966 a         3513 c         4041 g         2050 t
ORIGIN      
        1 tagagggtgg cctggcgcct ggtgagggtg cagaaggctt tgagttcgat cttgtctggc
       61 aggtcggtga tgatgctgcg gtcggtcttc atgcgccgca ggatgtagga ctgcacgagg
      121 cggcgcaggg gcgcgtaacc ttcagggctg ccggcgccgc gcttgacggc ctgggcaaag
      181 gcggcggcag ggccgagcag gccggggttg aggaaatcga aaaggctcca gagatcgccg
      241 gggcggttct cgaccggggt gccggtgagg gcgacgcggg cgggggcggc gagttttttg
      301 acggctttgg cggcgccgct gccggggttt ttgacggcct gggcttcatc gaggatgacg
      361 aggctccagg ggtggctcag ccagctttcg gtgcgctgga ggaactggta ggtggtgagc
      421 atggcatcac acccgcgcag ggcggcagca gggtctttgg cggcatggtc gagctgctcg
      481 cgcgggagct gggaggggtg ggcgatgagg actctcaact cgggggcgaa acgggcgagc
      541 tccgctttcc agttgccgat gagggaggct gggacgacga gaagggcggg gtcgccatct
      601 gctgtgggcg cgttcgccag aacgcgggag gcgtgtgagg agtcgggggc gttttccacg
      661 ctttggcaag cgcgcctgcg gctcagcagg agggcgatga cctggagagt tttgcccagg
      721 cccatgtcat cggcaaggca ggcgccgagg ccgaggcggg tcatgccgtg gagccaggcg
      781 aaacctttct gctggtaggg ccggagggtg gcgcgcaggc cgggcggcgg cggcatttcc
      841 ccaggggcgc ggatttcctc aagagcccgg gcgagcgctt tgcctgcgat gacctggctc
      901 cagtccggca cctgggcggc gctttcatcc ccgccggcgg cggcggcgcg cggatcgaag
      961 ccggcgagca gtctcatgcc gtcgaggaag ccgatgccgc cctcgcccgc ggcatgccgc
     1021 actttgcgcc agtgggccag cacctggcgg agctggtcac catccacctc gacccatctg
     1081 ccgcgcaagg aaacgaggcc ggtctcggcg ctgaggagct tttcccactc ggcttcggtg
     1141 aggggttcgc catcgaggct ctggctgatt ttaaaggaga gcaaggcgcc ggcgtggagg
     1201 gaggtctttt tcggcgcgtc cacggtgaca ctgacggcgg ggcgggggcc tttgccgccg
     1261 cgccaccagt cgggcagttt catgacgatg ccgctttcct ggagcgcggg gctttctttg
     1321 agcaggcgga aggcctcggg cggggtcatg gccaggggct ggaaaaggcg gcgcgtctcc
     1381 agccactcgc gcacgagccg ggagcgggcg gcggcggtgc gcacgggttc gagcagcgcg
     1441 gcgagcgcgg cctggtcttt ctgcccggcg taaagctgga gggcgcgcgc cagcgggagg
     1501 tgctggggct ggccggaagc gttgagcttt tcggtgaagg tggcgaggaa ggcgaagggg
     1561 cgttgagtgt cggtcttgtt ttccgccagg tggaaggtga cgcgtccgac ggcgtgccag
     1621 gcggggtttt cgaggcgcag ccagtccccg acaccgccag ggtgggctgc gatgagggcg
     1681 gtgacatgcc cggtcagggc ctgccaccag cgcagggcgg tctcggacgt gaggtgctcc
     1741 gagcctggaa agggaggcgc ggcggcgagg aaggcggcgg tctcgtccac agacggcggc
     1801 gtcactgcgg cggggtcgcg cgtctggcag aggcgggtga ggaagcggcg cggccactcg
     1861 cgcagccagc gccagggggc gtcgagttcg gcgtcgagca atcccgccgc cagcaggagc
     1921 agcagagcgg cctcgccttc aacagcggcg gcggcgagct tacgcccggt gctgtcctcg
     1981 ggcgcgcctt cgcagtggag aatgccttcg gggctgaggg cgggatgcgg catggagggt
     2041 tttcatgccc tggatggccg cctccggcaa gatcgcgcgg acggaaaaat ttttccgccc
     2101 aaaaaaaagg cttcacaaga aagactccgg ttcgtacgtt gtcggacagc cggacctcac
     2161 cggtctggca gtcaccacaa aaccaaaact agcataacac acatcatgaa gactctgttc
     2221 tccctcctcg ccgtcgccgg cttcgcctcc gccgccccgg tcaacaccga gtgccccgtg
     2281 gccggcaagc ccgccaagcc ctccgtgacg accacgcacg aaggcaagga aatcgccttc
     2341 tgctgcaaca actgcaaagg caagttcgaa gccgatccgg ccaagtacgc gaagaaaatc
     2401 aagtaaggcc ccggttgggt ctgcaaaagc ggagccgcgc gagcggctcc gcttttttgt
     2461 tgccagcggg tggcgcgccg ccggccggcc ttgcgcgttg acaatgcggc gcgccacctt
     2521 cacccttgga gcatcatgaa gcgtccaacg aagatcatca cagatccaaa aaaaatgttc
     2581 gagctttgct tgaatcagcc gcgcgtgtcg gcggcgcgga tgcgccagtc tattttggcg
     2641 cagaatggca gaacaagctc cccgcaatcc gtgaaagtct gagcgggtgg cagccggacc
     2701 tggaatgacc cggcatgggc atgacgcggc attttccggc aagccgccat ttgcccgcgc
     2761 ggcgcgtccg ctttatggac tgcggctcaa ccatcatgaa aaaagtcggc attggaatca
     2821 tcggcggcgg cctcatgggc cgggaaatgg cgagcgcttt cgggcgctgg tgcgcgctca
     2881 cggatgtgac tgtgcagccc gaactcgtgg ctgtggcgga tctagtggag ccggtgcggg
     2941 agtggttccg gcaggtgccc tcatgcgcgc agatcacggc ggattaccac gagctgctgg
     3001 cgaacccgtc ggtggacgtg gtgtatgtgg cggtgccgca caacctgcat gagaagattt
     3061 acacggatgt gctggcggcg ggcaaggacc tcttcgcgga gaagcccttc ggcatggacc
     3121 tggcctccgc gcgcaacatt ctggcggcgt gccggagcag cgggcgcttt gtgcgctgct
     3181 cgtcggagtt tccttttttt cctggggcgc agcgcgtgat cgcggaagtg ggctcgggcc
     3241 ggctgggacg tgttttggag gtggtctcgg gctttcatca cagcagcgac ctggatgcga
     3301 cgaagccggc gaactggaag cgcttttccc gcgcctgcgg cgagatcggc gtgctggggg
     3361 acctggggat gcacgcgtgc cacatcccgc tgcggctggg ctggcggcca aagcaggtct
     3421 ttgcccaact gcaaaaaggc tacccggagc gcccggatgg caagggcggc atggcggcgt
     3481 gcgacacatg ggacaacgcg ctgctgcact gctggacgga cattggcggc catgaggcgc
     3541 cgctgcgcct ggaaatgaag cgcctggctc ccggcgagac gaacacctgg ttcatcgagg
     3601 tgctcggcac cgagggcggc gcgcgctact cgacgaagga gccgaagacg ctctggatct
     3661 tcgagggcgg caaggtgcag tattggaaga agacggacct gggctttggc atgccgttca
     3721 aggcggtgac gggcggcatc ttcgagccgg gtttcccgga tgtgatccag cagatgtggg
     3781 cggcctttct catggagcgg gaaggaaagc tggacggccg gtttggctgc gccacgccgg
     3841 aggaggcggt ggccacgcag gagatcttcg ccgcggcgct ggagagccag aataaacgcg
     3901 cagtggtggc ggtgtgacgc gccgcctggg gagcgggcat cctgcctgct ctgaacaggc
     3961 gggacgcctg ttccccatgc atgatccatc cccgcaacct gccagccatg tccgcgttgt
     4021 tcatcccgcc cgactacttc cgcgagctgc ggcgggagga ggtctgcgcc gacgcggcgc
     4081 gtccgctgga ggtggagctg ggctgcgggg atggcacctt cctcgcgggc atggcggcgc
     4141 ggcacccgga gcgggagttt ttgggcatcg agcgcatgct tggccgcgtg ggcaagacga
     4201 tgcggaaaat cgagtccggc aggctggcga atgcgcgggt gatgcggctg gagagcggtt
     4261 acgcggccgg atggctgctg cccgcggcgg ccatcgcgcg cgtgcatctg ctgtgcccgg
     4321 acccctggcc gaagaagcgc cacacggcgc ggcggctggt gaaccaggag gagttcctca
     4381 acggcctggc gcgaatcctg gagccgggcg gggagttttt gctgaaaacg gacgacccgc
     4441 cctacttcga ggacgcgctg gccagccttg gcgcgcggcg ggagtttgag aggctcgaat
     4501 ggccggagga tgcgtttttc taccccgtca cggatttcga gagccactgg ctggcactcg
     4561 gcaaggaaat ccatcgcgcg cgctggcggc ggatgcggtg agtcccggca tcgggagtgg
     4621 gcatacgcat gagcgggccg gacgcgagtg gatcaaggct ccttggcgta gtcggcgtag
     4681 gcttcggcaa ttttggccgc tttttcatcg ccggtgtgga agatgaagcg gtggcgcagg
     4741 gtgatctttt cacccttctt gagggtgaag gctccggttt cggagttttt gtccagggat
     4801 tgcaaaccaa aggggttggc ggtgaagagg ccgtaggtgc gcacatgcca gggggtggga
     4861 tggcggaagc tggaggggtg gttgagcatg gccacgccca cagaggctcc tttcacggtg
     4921 ccgtggtagt ccacccaagt ggcgcgcttg gcccaggcgt cgccgtcggt gtggccctcg
     4981 ctgttgatga tgcggccggt gcccttgcct tccttgccct tgtccacggc catctcggag
     5041 gggacgcgga tgctgagtcc ggcgtctttt ttatcatcca cgagcgtgtc gccctcggtg
     5101 gcgatgagtt cgatgtccac gtcaatgata cgggcgccat ttttgaccat gaaagtatgg
     5161 cggcggattt cctcgatgat tttttttcct gcggcatcca catgatcggc cacgacggcg
     5221 agcacggcct tttcgccgtc cttcatttcg gtgatggacc ggtgcttgat ggcgccgaga
     5281 tggctgacac gggccgaggt cttgtcgctc ggcttttctc cgaatgtgct tcgctccgcc
     5341 caggtgtcgt atccaccgat gttttcatgg ccaaagcaga ggccgcgatg atggggatgg
     5401 tcgtgctgct cgccctcgac ggtcttcatg gggtagctcc gcgtcatgtc ggtgccatcg
     5461 gggccgatga cgggccacag gtaggttttg ttggcctggt ccacgacgaa ttcggtgaag
     5521 aactggccgt ccagcttcac gatggcgccg cccgcggcgg ttttttcgag ggtaaaattt
     5581 tccgcttggg cggaggcggt gacgagaagg gcggcgagga cgtggtggag cttcatggcg
     5641 cgggtaaaag ggcggagaag aacgcggagg caagcccgtt tcttccgccc ccgccacgcc
     5701 atgaaacatc tttttctacc ctgcctgctg ctctgcctgc ccgccttcga agccgccgcc
     5761 cagctccgcc cggacggagc cacggcctcc ttccgcggca gtctgccgcc caagcccgtg
     5821 gcggctctct ccgccgggca ggaggcggag ttggaaaagg aactggccgc tttgacagcg
     5881 gagttcgagc cggtgaaaaa acacccgcgc gcggcggacg cgcacatttt cctcaaggcg
     5941 gtgcgctacg cgctggactt cgacgagtgg tatgacaaga cgccggagga cggcctgaaa
     6001 aaagcccgaa agctgctggc cgaggctggc ggacgcatcg cggccttgaa gaaaaaccgc
     6061 gcgccgtgga tggacggacc gggctggaag gtgctcggct tttactcgcg cattgatgac
     6121 agcccgcagc cctacgcggt ggaggtgccg gaaggtctcg acaccggcaa aaagaaggcg
     6181 cccatgtgga tttggctgca cgggcgcggt gacacggcca ccgatttgca tttcatcgcg
     6241 ggcaggctcg gcagcgccaa accgggccag ttccagccgc agggaacgat cgtggtgcat
     6301 cccttcggac gctattgcaa cggctggaag agcgcaggcg agacggatgt ttttgaagtt
     6361 cgcgatgacg cggcggagcg cttcggcacg gacccggacc gcgtcgcgct cgcgggcttc
     6421 agcatgggcg gcgcgggcgc gtggcacatc ggcgcgcatt atccggacca gtgggcctgc
     6481 gtccacgcgg gcgccggctt tgtggatgtg cggcgatacc aaaaactgac gccggaaaaa
     6541 tatcccgcct ggcatgagca gaagctctgg ggcatgtatg acgtgcccga cgcggcgcgc
     6601 aatctgctca atgtgccgct cgtggtgtat agcggcgagg aggacacgca gcgcgacgcg
     6661 gcggcctaca tggaggaggt gctcgccaaa gagggcctcg tcatgcgcca ccacatcggc
     6721 cccggcatgg ggcacaaata tcattcggag gtgatcaaaa ccgtgcaggc ggagattgag
     6781 gcggcggttt ccaaaggccg cgaccgtttt cccaacgagg ttcgtgtcca ggcacgcaca
     6841 cccttttacg gccgcgtgaa atggctcaac ctgcacggaa tggaacggag ctgggaggag
     6901 gcgcgcattg acgcgcgcgt ggcaggcggg cgagccatcg aaatccagac gcgcaatgtg
     6961 aaccgcatcg tcatgtatcc gccgccgcag gcgcgcgcag gcggcgacaa actggatgtg
     7021 atcattgacg gggaaaaact gaccgtgtcg ctcggccccg tgctgccgga gttcgagact
     7081 ttcatgcggc ccggcccgcg cagcccggcc atgacggcgg cctttgtgcg cctggtcaaa
     7141 aaggacggac agtggaccga cttcggcggc gcgcagtcct tccagcacac cggccccgaa
     7201 ggcggaaaaa ccaccgcgca tcccggcccg atggactccg ctttcatgaa gcgcttcctc
     7261 gtcgtgctgc cggatggcag gtccagccat gccggcgtgg acgcctgggt gcaggcggag
     7321 tccgcgcgct tcatccagcg ctggcgcagc ctcatgcggg gcgacccgcg cgtgatccgc
     7381 gcggacgatc tggccgaccc cgtcgaggcc tgcaaaacgc agggcctcat cctctggggc
     7441 acgccggagt cgaactccgt gatccgcaag accgccgccc gcctgccgct gaactggggc
     7501 gcggaaaaaa tcgagatggc cggccaaagc catgacgccg ccacgcatgt gccattgatg
     7561 atttaccccg ctctggagtc gcctggcttc gaggtagtga tcaactcggg cctcaccttc
     7621 cgcgaggcgc atgaccgcac caactcgctg caaaatccca agctgcccga ctggaccatc
     7681 ctcgacatta cccagccgcc ggatgcggag aaagcgggca gggtcgtggc ggcggatttc
     7741 ttcgacgact tctggcgggt gaaatgaagc aggtttgcag tggtcaatgc actgccgcgg
     7801 tgaaacgcgt gcatactccc gtgccggcaa tgacggcggg tatctgctcg gcgtggcggc
     7861 cggaggcgat gacacattcg actcctgcgt tcacagcgtc ctggacggct ttcaatttgg
     7921 agatcatgcc gccagtgcca aggctggttt ttttttcctg cgcatggtgg atgacggcgt
     7981 ccacatcctc cacgagcggg atggggcccg cctcgggatg atccggatcc tgaagcaaac
     8041 cgggcgcgct ggtcagcagc aggagaacat ccgctcccca aagccgcgcg atgtgcgagg
     8101 agagcatgtc attgtcgcca aagcgcagtt cctcaatggc gacactgtcg ttttcattga
     8161 tgacaggaac gacctggggg aactcaagca ggcgctcaag cgtggccttg aaacgcgcgc
     8221 cacgctgggc ggcctccaaa tcctcatgcg tgacgagaag ttgcgccaca tgcagcccgt
     8281 ggtcgcgcag cagaccctcg taggcgtgca tgagatgggt ctggccgaca gcggccaggg
     8341 cctggagagt ggtcatgtca ttcggacgcg cggccatgcc catgggcttg agtccgctgg
     8401 ccaccgcgcc gctgctgacc acgacgacga tgtgccccgc ctgtttgaga agggccacgg
     8461 cctcgacgag tttgcgcaac tgcacggggt ccggctcggg catgctgacg cgggtgagga
     8521 tgccggagcc gaatttgatg acgatgcgct ttgagccggt gggggacatg aaaacagggc
     8581 gcgaagcatg gcacgggcct cccggctggc aacggggatt ctgcctcacg gttccgggcg
     8641 catcccgcgc gtcttaataa aagaacaggg cgcgcatctc gggtgtcagt tcggacgggc
     8701 gcagcttggt gacgccgctg gccgaggtca cggcgacacc tgtgttgtcc accttggtga
     8761 cgcgcacgtt gagcagtttt ctaccgtcgg ccaaaacgaa ctcggtgaag accctgccga
     8821 cctgcttttg acgcgcctca atgacggcgg cggctttttc ccggcccagg ttcacagtct
     8881 cagcgcgcgc ctggtcggcc ttggtcttga gcgcggcgac ctcggccttg cgaaactcca
     8941 ctttcttttg cagttccagc gcaccgatga tggcctgcat ctcggccctc cgtccggcca
     9001 cagtgccctc gtaagcctcg atgtttttca cggcctcatc caggccggcc ttcatttttt
     9061 tgaactggtc gtaaaagtac cagccggcta cggcgctggc gaaaatgatc agggcgagaa
     9121 ggtttttcat ggcgtcggct ccgtgatgaa tgagttgcgc gtgtcagttt tgcaggtgct
     9181 ttttgacctc ttcggtttcc gcccggagct tgtcgagagc ggcctgcgcc tcctcgtagt
     9241 ccctggtcat ggtgacaatc tgctggtcga ggatgtcaag ctccctctga aggctgagtg
     9301 tcatgcgctg cgcggagtcc atgggagagg aactgggcgg cacggccttg cgcaaatcca
     9361 caagcttttg ttcgaggccg tcatactcgg cccttaaggc ggccagtttt tccatggcgg
     9421 cggcgtggtc cgcctgcacc tgctccgctt ccttgcaact ggagagcatc agggcgcaaa
     9481 gaatgccaga caggacggct tggatgggac gaaggtcttt catgaggttt tccctttctt
     9541 ttgcgcggca ggctatccat ttggcgggat tgtgtcaatg cgtcagaaat ctttcccgaa
     9601 aaaactgtca gcggggactc gaagcccggc ctcaatgccg gctggtcatt ttaaggaaag
     9661 cgcggcttgc gagttgacaa acggcggcgg gtgaggcgag gtttccgcgc aatcaccccc
     9721 aaatcgtgtc cgcctcctat cacatttcca gaggagagaa acagcttggt ccatgttcgc
     9781 tggacgatct gcgcaccatg ctcgcctatg gctcggtgaa aaaaacggac atggtccggc
     9841 gcggggatgc gggtgactgg ctgccgcttg accgtctgga ggagcttcag caggaggaaa
     9901 gcgaggattc cgccgacccc aggcagagac ggcgcacggt gcgctaccgc gagtatcaca
     9961 aagtgccgca ggacagccgt gccggtgttg tcctgaaacg gctggcactg gggttcatct
    10021 tttttccacc catgctctgg cgcggagcgg tcaccgtctt ccaatcccgc gtggtgaccc
    10081 gccggaccaa tgagaacggt tatctcgaag tctggccgcg ctgggtggag ggcgccgtgt
    10141 cagcgatgat cgtggtcaac gcgctgggct ggtgggtgat gctctcctgg gccggcaggg
    10201 aggcggctcc aatggtgcgc gcgatcgccg gcaccatgtc ggttggtttt gatgtcctgc
    10261 gcgagtggct gggcagttga gaggcttcaa agattcctcc gaagcacgat cctcgcatcc
    10321 agcgccgtga agccgtcttg cgcggcaagg agcggattga tctccatctc cgcgatctgg
    10381 ggcaatgcca ggggtatcct gctgaacaag gctaccagcc gtgcgagcgc gcccatgtcc
    10441 agcggagccg agccgcgcgc gccgagcagt gcgcggtgga cgcgggtgcg cgcgatcagc
    10501 cgcagggcct gatccacgtc cagcggcgcc aaatccagcg ccgtatcctg cagcacatgc
    10561 accatcactc cgcccgcgcc aaagagcgcc acaggaccga actgcgcgtc ccgactggcc
    10621 ccgaggatca actccacgcc tttgtccgca atcatgcgct gcacagtgac tccgccaaaa
    10681 tgctccgcgc ccgccctggc cgccaccgcg tcgcgaattt cgcgccaggc atcgcgcacc
    10741 gcgtcctcgc cggtgagcgc cagcctcacc ccgccggcat cggttttgtg tgtcagcgtg
    10801 cgcgagcaga gcttcagcac cactggaaag ccgagcgccc gggcatgatc cacggcctcg
    10861 tcctccgagt ctgcgctcag agtctgaacc accgggatgc cgaaggcacc cagcagttgc
    10921 ttcgactccg cctcattcaa cagcacgcgc ccgccggcct ccgcccgcgc aatgacacgc
    10981 agcgccagat tcggcacggg ctcctcccca gcgcatgtgc cggacacggg cggctgtgac
    11041 agatgctctg tcacacgcgc gaacacacgc gccgccgtgt cgggatagtt gaaacacggg
    11101 atgcccgccc tggcgagaat gcgccgtcca gcctccacgc catcgccgcc catccagctc
    11161 gccagcagag gcttgccgga ttttttcgcc acctccacca cggcttccgc gaccgccgcg
    11221 ttgtcggtca tcgcctgcgg tgtcaggatg acgagcagcg cgtcattgcc tgcgtcctca
    11281 gccacgatct ccagcgcgcg ggcgtagcgc gcggcgtcag cgtctccgag aatgtccgcc
    11341 ggattcgagt ggctccaggg gccgggcaga aaggcgtcca gccgcgccag cgtctccgcg
    11401 ccgaggtccg ccggcgcgcc gccatgacgt gccagcatgt ccaccgccag cgcgccgggg
    11461 ccgcccgcgt tggtgacgat ggccagccgc cgtccgcgcg gcggtctgcg gcagggcagg
    11521 acctcggcgg tctggaaaag ctcggcaatg ctgtccacgc gcagcacgcc
//
LOCUS       DAOMYJ010000170        11416 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Prosthecobacter sp. isolate AMR_MDS_5629
            NODE_1884_length_11416_cov_21.175513, whole genome shotgun
            sequence.
ACCESSION   DAOMYJ010000170 DAOMYJ010000000
VERSION     DAOMYJ010000170.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867850
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prosthecobacter sp. (wastewater metagenome)
  ORGANISM  Prosthecobacter sp.
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Verrucomicrobiaceae; Prosthecobacter.
REFERENCE   1  (bases 1 to 11416)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11416)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.30
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:34:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,742
            CDSs (total)                      :: 4,684
            Genes (coding)                    :: 4,676
            CDSs (with protein)               :: 4,676
            Genes (RNA)                       :: 58
            tRNAs                             :: 55
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11416
                     /organism="Prosthecobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1884_length_11416_cov_21.175513"
                     /isolate="AMR_MDS_5629"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1965333"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..691)
                     /locus_tag="PLP58_15865"
     CDS             complement(<1..691)
                     /locus_tag="PLP58_15865"
                     /inference="COORDINATES: protein motif:HMM:NF019292.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+ dependent nucleoside transporter N-terminal
                     domain-containing protein"
                     /protein_id="HRJ10056.1"
                     /translation="MPAAGLLGLLAFPALAWALSENRRAFPWRALAAGLALQAALGWL
                     ILRTGAGRAFFQSLDDTFRTLLGFAGKGAAMVFGPLADEALLAEKWGPDNAFIFVVTV
                     TGTIVLVSALSAALYHYGVLQLVVRGMAWVMRRAMGTSGSESLAAAANVFMGQTEAPL
                     VVRPYLPGMTRSELLALMTGGMATIAGGVLAAYVSFGISAGHLLTASVMSAPAALMIA
                     KIMLPATAPAAS"
     gene            complement(728..1057)
                     /locus_tag="PLP58_15870"
     CDS             complement(728..1057)
                     /locus_tag="PLP58_15870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ10057.1"
                     /translation="MDERFPLPYQIGFIVLTALFAVAFSISREPRQWRRFWQSRFTRP
                     EDFSVNRNKYLDERLKHYGIVFAMIFLVLDVGVILTGLTHKARHESRLPTLEERLLKQ
                     RPATLEE"
     gene            1109..2110
                     /locus_tag="PLP58_15875"
     CDS             1109..2110
                     /locus_tag="PLP58_15875"
                     /inference="COORDINATES: protein motif:HMM:NF015509.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AEC family transporter"
                     /protein_id="HRJ10058.1"
                     /translation="MPGNLAAVFASGVMRSMGGRVLKLDFASILTVVLPVYLTMAAGA
                     AARRLKVLPQEADAGLMKLSITLLTPCLILERVAGNESVMSPLPVLLAAGLGYGLVAL
                     GIAVSFFAAPLIGLQKHAGRRTFAVSCGLQNYGFVAIPIVTSLFPDKGTLGVLFTFTL
                     GVELACWTAGVGLLTGLGRAPWRLALNPPVMAILFSLLLNFTGLHAWVPDVAHNTLGM
                     MGACAVPLSVLLIGASIHDVWGQEAMRWSVAVLAPVLRLALIPLAFLACAQWLPVTQE
                     LRNVLIVQAAMPSAVFNIVIARHYGGHAPTAVQCVLATTLVSLATTPLVLAWALRQV"
     gene            2146..3900
                     /gene="polX"
                     /locus_tag="PLP58_15880"
     CDS             2146..3900
                     /gene="polX"
                     /locus_tag="PLP58_15880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018290980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase/3'-5' exonuclease PolX"
                     /protein_id="HRJ10059.1"
                     /translation="MTREAAAEIFERIALLMELKGENPFKIRAYKTGAEIVESHPGDI
                     MQLAAENRLGGIKGLGEALQEKLHEMAATGRLDFYERLKAEFPEKLFELFGISGLGPK
                     KIAALHRDLGVGSLEDLRRVCENGQAAKLSGFGAKTVEKLLESIAFHEQHASEFRVDQ
                     VYALARTLVEELRALPEVSRAEVCGSFRRGKETVHDLDFLAASKKPEEVIAAFVKLPQ
                     VAKVIAQGGTKASIHTEEGVQCDLRVVSGAEYPFALVYFTGSKEHNVVLRQRALDRGW
                     SLNEYAFSHAEGRAGAPLPEEIHDEADIHRALGMDFIEPELRENSGEFEAAEKGRLPV
                     LVKLENLRGVFHNHTTASDGKATLREMAEAARELGLQYLGIADHSKSSFQANGLDERR
                     LRAQMRDIRELNEEFEGGFRIFTGTEVDILKDGSLDFSDEVLAELDYCVASVHNVFNL
                     PEAEMTKRIIRAIKNPHVTMLGHLTGRLLLQRPSYAVNVAAVIEAAAETGTIIELNAS
                     PWRLDMDWRWWRLAKEKGVKCSINPDAHSTRGLQDVIFGIRAARKGWLTRGDIINCLP
                     LAEVEKALRWKTEGGAED"
     gene            4080..4280
                     /locus_tag="PLP58_15885"
     CDS             4080..4280
                     /locus_tag="PLP58_15885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HRJ10060.1"
                     /translation="MATYVYETIPANSQEQPRRFEVRQKMSDPPLKTDPQTGEPVRRI
                     ITGGSGLVTHGASILSMKTRGR"
     gene            complement(4518..5117)
                     /locus_tag="PLP58_15890"
     CDS             complement(4518..5117)
                     /locus_tag="PLP58_15890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018969808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3341 domain-containing protein"
                     /protein_id="HRJ10061.1"
                     /translation="MSTTLKRVHGFLAEFDSVKDLYHAAEHVRDAGYQRWDVHSPFPI
                     HGMDHAMGVKRSKLPWLVFFGGMTGTCVAFFLQTITQTTFWSDIGLSFLQTLAETYPT
                     VVQGKPTHIWTLPAFFPVMFELTILFSAFTTLFGLMGLIGLPRLNHPLFTSKRFGKFS
                     DDGFFVCIEARDPKFSQEGTKSFLEKIGGKNVELVEDEI"
     gene            complement(5158..6645)
                     /gene="nrfD"
                     /locus_tag="PLP58_15895"
     CDS             complement(5158..6645)
                     /gene="nrfD"
                     /locus_tag="PLP58_15895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009960631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysulfide reductase NrfD"
                     /protein_id="HRJ10062.1"
                     /translation="MSEAVATAPHTADTHSGAPRILDREPLVLNQRSYSWITNRICGI
                     VENKQPALWWLLFIPSVLLTGLMVFCFVYLISTGVGVWGQKQPVAWAWDITNFVFWIG
                     IGHAGTLISAILFLTRQKWRTSVNRAAEAMTLFAVMCAGLFPAFHVGRVWMVWFLAPV
                     PNANAIWQNFKSPLLWDVFAVSTYFTVSVIFWYIGLIPDLATLRDRCKPGLKKVLYGM
                     FALGWRGANRHWSHYETAYMLLAALSTPLVLSVHSVVSFDFATSVVPGWHTTIFPPYF
                     VAGAIFGGFAMVLTLMIPVSKIYGLGDLITPKHIDNMAKIILLTGTIVGYAYCMEFFM
                     AYYSANKLELQTFQMRALHGPSIWAYIFMFSFNVFAPQLFWYRPFRHNLWVVMFVCMC
                     VNAGMWFERYVIIATTLERHLVPGSWRVYEATWVDKYTFLGTFGFFMMLFLLFLRFLP
                     VIAIGEVKGVLPQSNPHHDDHGKDGVQEEDLMGYPDRHVPREAAA"
     gene            complement(6789..10019)
                     /locus_tag="PLP58_15900"
     CDS             complement(6789..10019)
                     /locus_tag="PLP58_15900"
                     /inference="COORDINATES: protein motif:HMM:NF024247.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TAT-variant-translocated molybdopterin
                     oxidoreductase"
                     /protein_id="HRJ10063.1"
                     /translation="MKRIWNHPAEPQTGKRYWRSTAELEQRSEFLNKLGVEFPAGDTL
                     TEDERENSRRDFLKLMGASTAMMGLAACRRPITNILPYTNHVEWVVPGRPLLYATAMP
                     TANGATPVVAVTHEGRPTHLQGNPLHPLSGGLDAFAQSSVLDLYDPERSASPLVKGKK
                     AAWDEAGKELSGIIESAKKTSGADLALVVGGGNSPTLHRLLGEVKTALPQVRLFGYEA
                     VGNEGQASANKAVLGAGVKTFVRYSKALRILSLDCDFLSLDPQAGEPIKHFTRQRAKD
                     APGGEMNRLYVLENRYTLTGGMADHRKPLAASLIPAAAAFIAETLGDSSAKALADTAT
                     PGVREWLGPVIRDLVDHKGKALVLAGSRHGAEVHALVASINNALGAYGSTIELRQSSG
                     ETELGGSAELAAAVSSGAVKTLVSLTPSNLLFDAPASLAAAIEEKGVQVVHLGHLANS
                     TARKAALHIPAAHYLESWGDVRAADGTYSVVQPMIQALYDGASEIELLLAILGRKTLG
                     PVQPPADGAPAPEDPAHQAVRDTFAIVAKKLDEEKWNHTLRDGFLKGSAWPKAASEAN
                     TAAVAGIVAKARPAAAPSEEAMEVVLVPDAGVYDGRYISNAWLQEAPDPVTKLSWDNA
                     AWIGSATFRALKLKEGQMVKITVGDAELKLPAIEAPGHVSNSITIPLGYGEKGLGFVA
                     SDTKNKGRGFNAYPLRKSATEFVLTGAKIEKTGDIYELAVTQEHNTMEGRALYREATL
                     ETFNKTPDFVEKTGMDSHIPQNISFYKGQIGRKTETNPEGFDYDTKHQWGMTIDLSKC
                     LGCTACVIACQSENNIAVVGKDQVRKGRLMQWIRMDRYFASATWGEQNAKRDDVNISP
                     TVEQLENAEMVQQPVACQQCESAPCETVCPVNATVHTDDGLNAMAYNRCIGTRYCANN
                     CPYTARRFNWFDYNKRPLDELYLGPLSSKDKTGVRESLQLQKNPNVTVRMRGVIEKCT
                     YCVQRLESAKILQKQKQRDSKDFRVPTDTIKVACQAACSADAIVFGDLADPKSAVNKS
                     KASPRNYTLLKYIGTQPRTSYLARLKNPNPAMPGHENVAAWSAHQI"
     gene            complement(10056..10859)
                     /locus_tag="PLP58_15905"
     CDS             complement(10056..10859)
                     /locus_tag="PLP58_15905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009960628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c3 family protein"
                     /protein_id="HRJ10064.1"
                     /translation="MGNFFPRWTNWLPLKLAIGAAFIAFGVSVGVAYYFTPKYTRVGY
                     EPTQPVPFSHKIHAGQLGLDCRYCHSFVEQSGHANVPTNQTCFNCHGPGKGNIKADSP
                     KLELVRKAQETGEPIPWVKVHKAPDYVYFNHSAHLNRGISCQSCHGKVNEMEVVKHAE
                     PQSMGWCLDCHRNPEQNLRPLDQITNLNYKPGDLDRAAFYQSLLGKGVKADEIAATIQ
                     EDGKASSLDEILALASSTYGKNVTQLEVGSQLKKHWQVMPPENCTACHR"
     gene            11045..>11416
                     /locus_tag="PLP58_15910"
     CDS             11045..>11416
                     /locus_tag="PLP58_15910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ10065.1"
                     /translation="MTDEDRFRTAWHLRARRTRAKANLGWWLARLAPLLALAGLAGFA
                     LILSLRALADGAGWSGFLPWMTGTLMLAAGLAWLLARRDFICETQALVRLESELGLHN
                     ALTAAAAGRGPWPQPPERCDDG"
BASE COUNT         2062 a         3144 c         4148 g         2062 t
ORIGIN      
        1 gcgaggcggc gggcgcggtc gcgggcagca tgattttggc gatcatgagc gcggcggggg
       61 cgctcatgac ggaggcggtg agcaggtgcc cggcggagat gccgaaggag acgtaggcgg
      121 caagcacgcc gccggcgatg gtggccatgc cgccggtcat gagggcgagc agctcgctgc
      181 gcgtcatgcc gggcaggtag ggccggacga cgagcggggc ctcggtctgc cccatgaaga
      241 cattggccgc ggcggccagg ctctcgctgc cgctggtgcc catggcgcgg cgcatgaccc
      301 aggccatgcc gcgcacgacg agctggagca cgccgtagtg gtagagcgcg gcggagaggg
      361 cggagacgag gacgatggtg ccggtgacgg tgacgacgaa gatgaaggcg ttgtcaggcc
      421 cccatttctc ggccagcagc gcctcgtcgg ccagcgggcc gaagaccatg gcggcgcctt
      481 tgccggcaaa gcccagcagg gtgcggaagg tgtcgtccag gctctggaaa aaggcgcgcc
      541 ccgcgcccgt gcgcaggatg agccagccaa gcgcggcctg aagcgcgagt ccggcggcaa
      601 gggcgcgcca gggaaaggcg cggcggtttt ccgaaagcgc ccacgccagc gcgggaaagg
      661 cgaggagtcc gagaagtccg gcggcgggca tggcggggag tgaggcggga ttcgcgccgg
      721 gcgggcgtca ttcctccagg gtggcgggcc gctgcttgag caggcgctcc tccagcgtgg
      781 gcaggcggga ttcatggcgc gccttgtgcg tcagcccggt gaggatgacg ccgacgtcga
      841 gcacgaggaa gatcatggcg aagacgatgc cgtagtgctt gaggcgctcg tcgaggtatt
      901 tgttccggtt cacggagaaa tcctccggcc ttgtgaagcg cgactgccag aagcgccgcc
      961 actggcgcgg ctcgcgcgag atgctgaagg ccacggcaaa gagcgccgtg agcacgatga
     1021 agccgatctg gtaaggaagt ggaaaacgct cgtccatggc gggccgcccg gcttcataaa
     1081 acaaagcgcg cgcaatggca acgcgggaat gccggggaat ttggcggcgg tgtttgccag
     1141 cggcgtcatg cggagtatgg gagggcgcgt tttgaagctc gatttcgcca gcatcctcac
     1201 cgtggtcctg cccgtctatc tgacgatggc cgcgggcgcg gcggcgcggc ggctgaaggt
     1261 gctgccccag gaggcggatg ccggactgat gaagctgtcc atcaccctgc tcacgccctg
     1321 cctcatcctg gagcgcgtgg cgggcaatga atcggtgatg agcccgctgc cggtgctgct
     1381 ggcggcgggg ctgggttacg gcctggtggc gctgggcatc gcggtgtcgt ttttcgccgc
     1441 gccgctcatc gggctgcaaa agcacgcggg caggcgcacc ttcgccgtga gctgcggact
     1501 gcaaaactac ggcttcgtgg ccatccccat cgtcacctcg ctgtttccgg acaagggcac
     1561 gctgggcgtg ctcttcacct tcaccctcgg cgtggagctg gcctgctgga cggcgggcgt
     1621 gggcctgctc accggcctgg gcagggcgcc ctggcggctg gcgctgaacc cgcccgtgat
     1681 ggccatcctt ttttccctgc tgctgaactt caccggcctg catgcctggg tgcccgatgt
     1741 ggcgcacaac actctcggca tgatgggcgc ctgcgccgtg ccgctgtccg tgctgctcat
     1801 cggcgcgtcc atccacgatg tctggggcca ggaggccatg cgctggtcgg tggccgtgct
     1861 ggctcccgtg ctgcgcctgg cgctcatccc gctggcattc ctggcctgcg cgcaatggct
     1921 gcccgtgacc caggagctgc gcaacgtgct catcgtccag gccgccatgc caagcgccgt
     1981 cttcaacatc gtcatcgccc gccactacgg cggccacgcc cccaccgccg tccagtgcgt
     2041 cctggccacc accctcgtca gcctcgccac caccccgctc gtgctcgcct gggcgctgcg
     2101 gcaggtgtga tttgtctttt gcggcgggcg cgccagactg ggcggatgac ccgggaagct
     2161 gccgctgaga tctttgagcg catcgcgctg ttgatggaat tgaaggggga gaaccctttc
     2221 aaaatccgcg cctacaagac gggggcggag atcgtggaga gtcatccggg ggacatcatg
     2281 caactggcgg cggagaaccg gctcggaggc atcaaggggc tgggggaggc gctgcaggag
     2341 aagctgcacg agatggcggc gacggggcgg ctggactttt atgagcggct gaaggcggag
     2401 ttcccggaga agttgttcga actgtttggc atcagcggcc tggggccgaa aaagatcgcg
     2461 gcgctgcatc gcgacttggg cgtgggcagc ctggaggatc tgcgccgggt gtgtgaaaac
     2521 gggcaggcgg cgaagctctc cggcttcggg gcgaagacgg tggagaagct gctggagagc
     2581 atcgctttcc acgagcagca cgcttcggag ttccgcgtgg accaggttta tgctctggcg
     2641 cggacgctgg tggaggagct gcgcgcgctg cctgaggtgt cgcgcgcgga ggtgtgcggc
     2701 agcttccgcc gggggaagga aacggtgcat gacctggact tcctggcggc ctcgaagaag
     2761 ccggaggagg tgatcgcggc ttttgtgaaa ctgccgcagg tggcgaaggt gatcgcgcag
     2821 ggcggcacga aggcgagcat ccacacggag gagggcgtgc aatgcgacct gcgcgtggtg
     2881 agcggcgcgg agtatccttt tgcgctggtg tatttcaccg gcagcaagga gcacaatgtg
     2941 gtgctgcgcc agcgcgcgct ggaccgggga tggtcgctga atgaatacgc attcagccat
     3001 gcggagggca gggcgggcgc gccgctgccg gaggagattc atgacgaggc ggacatccat
     3061 cgcgcgcttg ggatggactt tattgagccg gagctgcggg agaattcggg cgagttcgag
     3121 gcggcggaaa agggccgcct gccggtgctg gtgaagctgg aaaatctgcg cggtgttttc
     3181 cacaaccaca cgacggccag cgacggcaag gccacgctgc gggagatggc ggaggcggcg
     3241 cgggagctgg gtttgcaata cctgggcatc gccgaccaca gcaagagcag cttccaggcg
     3301 aacggtctgg atgagcggcg gctgcgcgcg cagatgcgcg acatccgcga gttgaatgag
     3361 gagttcgagg gcgggttccg cattttcacc ggcacggaag tggacatcct caaagatggc
     3421 agcctggact tctccgacga ggtgcttgcg gagctggact actgtgtggc gagcgtccac
     3481 aacgtcttca acctgccgga ggcggagatg acgaagcgca tcatccgcgc catcaagaat
     3541 ccgcacgtca ccatgctcgg ccatctgacg gggcggttgc tgctccagcg gccctcctac
     3601 gcggtgaatg tggcggcggt gatcgaggcg gcggcggaga cgggaacaat catcgagctg
     3661 aatgccagcc cgtggcggct ggacatggac tggcgctggt ggcggctggc aaaggagaag
     3721 ggcgtgaagt gcagcatcaa tcccgacgcg cacagcacgc gcggcctgca ggacgtgatc
     3781 ttcggcatcc gcgcggcgcg caagggctgg ctgacgcgcg gagacatcat caactgcctg
     3841 ccgctggcgg aggtggagaa ggcgctgagg tggaaaacag aaggcggagc ggaggattga
     3901 actttagggg cacgggcggg ccggcagtcg aacaacctgc caggctccgc gccgcccgcg
     3961 cccgcggaag aatggggcgc ggcacgtcac tttcccgccg caatggcgtt tccgttcgcc
     4021 ctatttcgag cttgaaacaa aacggcttcc caacacgatt tcacgatcca ttcaccacca
     4081 tggccaccta cgtttacgaa actatccccg cgaattccca ggaacagccg cgccgcttcg
     4141 aggtaaggca aaaaatgagc gatccacccc tgaagaccga cccgcagacc ggcgaacctg
     4201 tccgccgcat cattaccggc ggcagcgggc tcgtcacgca tggcgcgagc attctctcaa
     4261 tgaaaacacg aggaagataa gccagtgttg tcgcgggatg aatgcgcggg ccgggcgtgc
     4321 gaaaacggcg gcatcatggg aacaaggggg caattactcc gctcccgtgc ccgtaatcct
     4381 tgacgcagac cagcctcatc aaaaactatg ccctcgcggc aagtcggcca agctgatgac
     4441 ggcgctgagg gtaaacaggg attccgcgct ttgccggcgt gcattgacac ggcaagacct
     4501 gggaacaccc gccgggatca gatttcgtcc tcgacgagct cgacgttttt gccaccgatc
     4561 ttctccagaa aggacttggt gccctcctgg gagaatttgg ggtcgcgcgc ctcaatgcag
     4621 acgaagaagc cgtcgtcgga gaatttgccg aagcgtttgg aggtgaacag gggatggttc
     4681 agacgcggca ggccgatgag gcccatgaga ccgaagagcg tggtgaaagc cgagaagagg
     4741 atcgtcagtt cgaacatcac cgggaagaaa gcgggcagcg tccagatgtg ggtgggcttg
     4801 ccctgaacca cggtggggta ggtctcggca agcgtttgaa ggaagctcag accgatgtca
     4861 gaccagaagg tggtctgggt gatggtctgg aggaagaagg ccacgcaggt accggtcatg
     4921 ccgccgaaga agacgagcca gggcagcttg gagcgcttca cacccatggc gtggtccatg
     4981 ccgtggatgg gaaatggcga gtggacatcc cagcgctggt agccggcgtc gcgcacatgc
     5041 tcggcggcgt ggtagaggtc cttcacgctg tcgaactcgg ccaggaaacc atgaactcgt
     5101 ttgagggtgg tgctcacgtg gatttcagat attggatttc aaagtttcaa atgcggctca
     5161 ggcggcggcc tcgcgcggga catgacggtc gggatagccc atgagatcct cctcctggac
     5221 gccatctttg ccgtggtcgt catgatgggg gttggactgc ggcagcacgc ctttgacctc
     5281 gccgatggcg atgacgggca ggaagcgcag gaagaggagg aagagcatca tgaagaagcc
     5341 gaaggtgccc aggaaggtgt atttatccac ccaggtggcc tcatagacgc gccaggagcc
     5401 gggaacgagg tggcgctcca gggtggtggc gatgatgaca tagcgctcga accacatgcc
     5461 ggcgttcaca cacatgcaga caaacatgac gacccagagg ttgtgccgga agggacggta
     5521 ccagaagagc tgcggcgcga agacgttgaa gctgaacatg aagatgtaag cccaaatgga
     5581 ggggccgtgc agcgcgcgca tctggaaagt ctgcaactcc agcttgttgg cgctgtagta
     5641 ggccatgaag aactccatgc agtaggcgta gccgacgatg gtgccggtga gcaggatgat
     5701 cttcgccatg ttgtctatgt gcttgggcgt gatcagatca cccaggccat agatcttcga
     5761 gacggggatc atgagcgtca gcaccatggc gaaaccgccg aaaatggcgc ccgcgacgaa
     5821 gtaggggggg aagatggtgg tgtgccagcc gggaacgacg gaggtggcga agtcaaagga
     5881 caccacggaa tgcacggaga gcacaagcgg ggtggaaagc gcggccagga gcatgtaggc
     5941 ggtctcgtag tggctccagt ggcggttggc gccgcgccag ccgagggcga acatgccgta
     6001 aaggactttt ttcaggccgg gcttgcagcg gtcgcgcagg gtggcgagat cgggaatgag
     6061 gccgatgtac cagaagatga cggagacggt gaagtaggtg gagaccgcga acacgtccca
     6121 aagcaggggg gacttgaagt tctgccaaat ggcgttggcg ttcggaacgg gggcgaggaa
     6181 ccagaccatc cagacgcgcc cgacgtggaa agcggggaac aggcccgcgc acatgacagc
     6241 gaagagggtc atggcctcgg cggcgcggtt gacggaggtg cgccacttct ggcgggtgag
     6301 gaagagaatg gcggaaatga gcgtgcccgc gtggccaatg ccgatccaga acacgaagtt
     6361 ggtgatgtcc caggcccagg cgacaggctg cttctgcccc cagacaccga cgccggtgct
     6421 gatgagatag acgaagcaga agaccatgag gccggtgagc agcacggagg ggatgaaaag
     6481 cagccaccag agggcgggct gcttgttttc cacaatgccg cagatgcggt tggtgatcca
     6541 ggagtaggag cgttggttga gcaccagcgg ctcacgatcc aggatgcgcg gcgcgccgga
     6601 gtgagtgtct gcggtgtggg gagcggtggc gactgcttcg gacatgtcag cgggtgaggg
     6661 gaaaatttgg aagctcgaaa ttcggggagc cgcaaaccat cctgccgtgg acaaggtgga
     6721 tggattgcag gcctgcgggg ttcatgaaat gcggttgtct cgggtttcgt gcttcgaact
     6781 tcgatttttc aaatctgatg ggcgctccag gcggcgacgt tttcgtgtcc gggcatggcg
     6841 gggttcggat tcttgagccg cgccagatag ctcgtgcgcg gctgtgtgcc gatgtatttg
     6901 agcagggtgt agttgcgcgg ggaggccttg gatttgttga cggcggattt cgggtcggcg
     6961 aggtcgccga agacgatggc gtcagcggaa caggccgcct gacaggcaac cttgatggtg
     7021 tccgtgggca cgcggaagtc cttcgagtcg cgctgcttct gcttttgcag gatcttggcg
     7081 gactcgagac gctgcacgca gtaggtgcat ttctcgatga cgccgcgcat gcggacggtg
     7141 acgttggggt tcttctggag ctggagtgac tcgcggacgc cggtcttgtc cttggaggaa
     7201 agcgggccga ggtaaagctc gtcgagcggg cgcttgttgt agtcgaacca gttgaagcgg
     7261 cgcgcggtgt aggggcagtt gttggcgcag tagcgggtgc cgatgcagcg gttgtaggcc
     7321 atggcgttga ggccgtcgtc ggtgtggacg gtggcgttga cggggcagac ggtctcgcat
     7381 ggagcggact cgcactgctg gcaggccacg ggctgctgga ccatctcggc attttcgagc
     7441 tgctcgacgg tgggggagat gttcacgtcg tcacgcttgg cgttctgctc gccccaggtg
     7501 gcgctggcga agtagcggtc catgcggatc cactgcatga ggcggccttt gcgcacctgg
     7561 tccttgccga cgacggcgat gttgttctcg ctctggcagg cgatgacgca ggcggtgcag
     7621 ccgaggcact tgctgaggtc aatggtcatt ccccactggt gcttcgtatc gtagtcgaag
     7681 ccttcggggt tggtctcggt cttgcggcca atctgccctt tgtagaacga gatgttctgc
     7741 gggatgtggc tgtccatgcc cgtcttctcg acgaaatccg gcgtcttgtt gaaggtctcc
     7801 agggtggcct cgcgatagag ggcgcggccc tccatggtgt tgtgctcctg ggtgacggcc
     7861 agttcgtaaa tgtcgccggt tttttcgatc ttggcgccgg tcagcacgaa ttcggttgcg
     7921 gatttgcgca gcgggtaggc gttaaaaccg cggcctttgt tcttcgtgtc agaggcgaca
     7981 aaaccaagac ctttctcacc ataaccgagc gggatggtga tggagttcga gacatggccg
     8041 ggggcctcaa tggcgggcag cttcaactcg gcgtcgccaa cagtgatttt caccatctgg
     8101 ccttccttga gcttgagcgc gcggaaggtg gcggagccga tccaggcggc gttgtcccag
     8161 ctcagtttgg tgacgggatc gggggcctcc tggagccagg cattgctgat gtagcgtccg
     8221 tcatagacgc cggcatcggg cacaagcacg acctccatcg cctcctccga gggagcggca
     8281 gcgggcctgg ccttggcgac gatgccagcc acggcagcgg tgttggcctc gctggcggct
     8341 ttcggccagg cggagccttt gaggaagccg tctcgcaggg tgtggttcca tttttcctcg
     8401 tcgagctttt tcgccacgat ggcaaaggtg tcgcgcactg cctggtgggc ggggtcctct
     8461 ggcgcgggtg cgccgtcggc gggaggctgg acgggaccga gcgttttgcg tccgagaatg
     8521 gcgagcagga gttcgatctc gctggcgcca tcataaagcg cctggatcat cggctggacg
     8581 actgagtatg tgccgtcggc ggcgcggaca tcgccccagg attcaaggta atgcgcggcg
     8641 gggatgtgca gggcggcctt gcgcgcggtg gagttggcaa gatggccgag atgcacgacc
     8701 tgcacgcctt tttcctcaat ggcggcggcg agggaggccg gggcgtcaaa aaggaggttc
     8761 gagggtgtta gggagacgag ggttttcacc gcgccggaac tgacagcggc ggcgagttcc
     8821 gcggagccgc cgagttcggt ctcgccgctg gactggcgca actcgatggt gctgccgtag
     8881 gcgccgaggg cgttgttgat agatgcgacc agcgcgtgaa cctcggctcc atggcgggag
     8941 ccggcgagca cgagggcttt gcctttgtgg tcaacgaggt cgcggatgac cgggcccagc
     9001 cactcgcgca ctccgggagt ggcggtgtcg gcaagggcct tggcggagct gtcgccgagt
     9061 gtctccgcga tgaacgcggc ggcggcgggg atgaggctgg cggcgagcgg cttgcggtgg
     9121 tcggccatgc cgccggtgag ggtgtagcgg ttctccagga cgtagaggcg gttcatctct
     9181 ccgccagggg cgtctttggc gcgctgcctg gtgaaatgct tgatcggctc gccagcctgg
     9241 ggatcaaggg agaggaaatc acagtcgagg gaaagaatgc gcagggcttt gctgtagcgg
     9301 acgaaagtct tcacgccggc gccaaggacg gccttgtttg cggaggcctg gccctcgttg
     9361 ccgactgcct catagccgaa cagcctgact tgcggcaagg cggttttcac ctcgccgagc
     9421 agacgatgca gcgtgggcga attgcctccg ccgaccacca gggcgagatc cgcgccgctg
     9481 gtctttttgg cggattcaat gatgccggaa agttctttgc ctgcctcgtc ccaggcggct
     9541 tttttgcctt tgaccagggg actggcggag cgctcggggt catacaaatc gagcacggaa
     9601 gactgggcga aagcgtcgag accgccgctc agaggatgca gcggattacc ctgcaaatgg
     9661 gtggggcggc cttcgtgcgt gacggcgacc acgggtgtcg cgccattcgc ggtgggcatg
     9721 gcggtggcat aaagcagcgg acggcccgga acgacccact cgacatgatt ggtgtagggc
     9781 aggatgttgg tgatggggcg gcggcaggcg gcgaggccca tcatggcggt ggaggcgccc
     9841 atgagcttga ggaagtcgcg ccgggagttt tcacgctcat cctcggtgag ggtgtcgccg
     9901 gcggggaatt cgacgccgag cttgttcaga aactccgagc gctgctcaag ttcggcggtg
     9961 ctgcgccagt agcgcttgcc ggtttgaggc tcagcggggt gattccagat gcgtttcatg
    10021 tgggcgggaa aggagtcgtg aaaaatgagc gcggatcagc ggtggcaggc ggtgcaattc
    10081 tcgggcggca tgacctgcca gtgctttttg agctgggagc cgacttcgag ctgggtgacg
    10141 tttttgccgt aagtggagga ggcgagggcg aggatttcgt ccaggctgga agccttgccg
    10201 tcctcctgga tggtggcggc gatttcatcc gccttcaccc ccttgcctag caggctctga
    10261 taaaaggcgg cgcggtcgag atcaccgggc ttgtagttca aattggtgat ctggtcgagc
    10321 ggacgcaagt tttgctcggg attgcggtgg caatcgaggc accagcccat ggactgcggc
    10381 tcggcatgct tgacgacttc catttcattg accttgccgt ggcatgactg gcaggagatg
    10441 ccgcggttca agtgggcgct gtgattgaaa taaacatagt ccggcgcctt gtggactttc
    10501 acccacggaa tcggctcgcc agtctcctgg gccttgcgca cgagctcgag tttcgggctg
    10561 tcggccttga tgttgccctt gccgggtccg tgacagttga agcaggtctg gttggtgggc
    10621 acgttggcgt ggccggactg ctcgacgaag gaatggcagt aacggcagtc aaggccgagc
    10681 tggccggcat gaattttgtg ggagaacgga accggctggg tcggctcgta gccgacacgg
    10741 gtgtatttgg gcgtgaaata ataggccacg cccacgctga cgccgaatgc aataaacgcg
    10801 gcgccgatgg cgagcttcag aggcagccaa ttggtccagc gcggaaagaa gttacccata
    10861 atttgtcgtt ttctgagctt gtgaaaaatt tcacaagcgg cgttcagaat aaaagtttga
    10921 aagggctttg caaccctgga aatcattttt ttgaacgcgc tgcccggcct gtccaaccgc
    10981 cagggatcaa gggcggcaaa atttttttct cgtcggaatc atccctggag cctatgtatg
    11041 cggcatgact gacgaagacc ggtttcgcac cgcatggcat ctgcgcgccc gccgcacgcg
    11101 cgccaaggcg aatctgggct ggtggctggc gcggctggcc cccctgctcg cgctggcggg
    11161 actggcgggc ttcgcgctca ttctgtcgct gcgcgcgctg gctgacggcg ctggctggtc
    11221 tggcttcctt ccgtggatga ccgggacgct gatgcttgcg gcaggtttgg catggttgct
    11281 ggcgcgacgc gacttcatct gcgaaacgca ggcgctcgtg cgcttggaat ccgaactcgg
    11341 cctgcacaac gcgctgacag cagccgccgc agggcgtggc ccgtggccgc agccgccgga
    11401 gcgctgcgac gacggc
//
LOCUS       DAOMYJ010000171        11362 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Prosthecobacter sp. isolate AMR_MDS_5629
            NODE_1896_length_11362_cov_22.354205, whole genome shotgun
            sequence.
ACCESSION   DAOMYJ010000171 DAOMYJ010000000
VERSION     DAOMYJ010000171.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867850
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prosthecobacter sp. (wastewater metagenome)
  ORGANISM  Prosthecobacter sp.
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Verrucomicrobiaceae; Prosthecobacter.
REFERENCE   1  (bases 1 to 11362)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11362)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.30
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:34:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,742
            CDSs (total)                      :: 4,684
            Genes (coding)                    :: 4,676
            CDSs (with protein)               :: 4,676
            Genes (RNA)                       :: 58
            tRNAs                             :: 55
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11362
                     /organism="Prosthecobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1896_length_11362_cov_22.354205"
                     /isolate="AMR_MDS_5629"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1965333"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..447)
                     /locus_tag="PLP58_15915"
     CDS             complement(<1..447)
                     /locus_tag="PLP58_15915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ10066.1"
                     /translation="MNYLRSILRFTVPLLILGGCGYAAWWLLAHPPEQEKKSVPAPLV
                     RVEGTVLRKGLYEPRVRSQGTVRPRTRSSLLPEVAGKIVEVSPAFRPGGFFEKDEVLL
                     RLDPLDYETAVVIAQAAQAQAEALLAEEQARADQAVENWKALGRAGL"
     gene            complement(505..2391)
                     /locus_tag="PLP58_15920"
     CDS             complement(505..2391)
                     /locus_tag="PLP58_15920"
                     /inference="COORDINATES: protein motif:HMM:NF012869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRJ10067.1"
                     /translation="MSAAKHSNSLAPITEAELKHLKMPYRRLLPYLRPWLGRFFLGMG
                     LGALAASFNAVMILGLQLIFQLVLDGASKDLSMPVEMAFVGKINLGEMLGLEPGTPMG
                     LWGVIAACACIPLLMFLSGVLEYLNKYCLAWVGTKMLFQIRSDVFAAIMRQSPAFFAT
                     TKAGELMQVVSHQSRVAQSHALQVVQLISNRPLTIIVILATLFAMDWFFTLMSLVVFP
                     LCLAPILVIGRRVRKVGAREEKEVGAMMTAMHESFTGIRLVKANAREDYECKRFDRAN
                     REMTRNILRWTRATEMIGPIVEAVASIGIAAGLVYFWWKERPAHELIVLVLALSKIYP
                     PAKELSKVSLLMQKTVYAINNIFRVLDRQPDITDAPDARRLPRVSGAIRFDEVSFAYH
                     APSGAKLEKAAVRGVSLDLQPGRFYALVGPSGAGKSTLFSLLLRFYDPDSGRVLLDGE
                     DIRLATVSSLRENIGMVSQDTFLFHDTIRENIRYGRLDAKEDEILAASKQAHAHEFIQ
                     QIQGGYDAVVGDAGCNLSGGQKQRISIARAILRNAPVLLLDEATSALDTETEKIIQEA
                     IETLAQGRTVIAIAHRLSTILEADQIIVMNEGRVEAVGPHQTLLQTSPLYQKLYQLQY
                     QNGG"
     gene            2514..3359
                     /locus_tag="PLP58_15925"
     CDS             2514..3359
                     /locus_tag="PLP58_15925"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013213.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HRJ10068.1"
                     /translation="METSRHPSSTVLFDSHNHLHDARLLPWRAEIMAELPALGIAGVV
                     ANGTCEQDWPAVAELARAHPWVRPSFGLHPWRVSGRGPEWKETLEAFLAEFPGAAVGE
                     IGLDRWMEQPDVEAQRECLLWQLDLAVRLERPVTVHCLRAWGLLDEVLRAARLPERGF
                     LLHSYGGPAEMIPGFVKMGAFFSCSPWFGHPAKSAKLAVFKSVPADRLLIETDAPDMA
                     PPEELDTHPLWHEGLRLNHPANLRVSLSLLSRLRGTPGDELAAQIAANHRSLFGECGV
                     TRDAR"
     gene            complement(3340..5592)
                     /gene="pbpC"
                     /locus_tag="PLP58_15930"
     CDS             complement(3340..5592)
                     /gene="pbpC"
                     /locus_tag="PLP58_15930"
                     /inference="COORDINATES: protein motif:HMM:TIGR02073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009274 - peptidoglycan-based cell wall
                     [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 1C"
                     /protein_id="HRJ10069.1"
                     /translation="MRRRLTVFSAALLAGALAAWFGAPWLAPLPPALLAAQSVSTRFL
                     AADGTPLRMLLTEEGQRVAGTASYADIPEHLRNAVLAAEDKRFFQHGGVDLLAIARSA
                     WDNLRAGRVVSGASTLHQQLIKITAARPGPRTLGVKLVEALQARRLAMTWSKEEVLAA
                     WLNRVSYGNLLTGCESAALGYFDKPLPDLSPAECALLAAIPQSPARLNPFRGIERARP
                     RQTRILEKMRALGWLDAGQLQTALAEPVRLRRFNGGFEAPHAVQMLAGGTAPDPVVLT
                     TLDAALQRAVERVITERLSGLRERQVTQAAVVVIENATGHILALAGSRDFSAPDGGQI
                     NGAWTPRSPGSAVKPFTYLLAFERGATPASIVADLPVEYSTGSGSYLPENYTLKHYGP
                     MTYRAALGNSLNVSAVKVLQSAGGAAELLHRLRELGLTTLTESAEHYGPGLTIGNAPV
                     RLVELANAYACLARLGIHKPWSLLPRENEGARLLDGTLCYLIADILADNSARALTFGQ
                     NSVLRLPFPAAVKTGTSSTYRDNWTLGFTREFTVGVWAGNFDNRPMQDVSGVTGAAPI
                     WRDVMLELQRRRGVSWHEPPPGLVKARVDPRNGRRLTPQSPPARLSREDFFPPRRLPP
                     PAGAEDYDAQGRAILPGIYADWVAGADNWLGDLVVCETAKKEKRPWQITNPVPGTVIH
                     LDPDLPGSGARLLLQANQQGVRWDCGTLEIVDDAGQPVALLKPGRHTLRAEDPATGET
                     RRTFVIVHPE"
     gene            7002..10694
                     /locus_tag="PLP58_15935"
     CDS             7002..10694
                     /locus_tag="PLP58_15935"
                     /inference="COORDINATES: protein motif:HMM:NF019325.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CARDB domain-containing protein"
                     /protein_id="HRJ10070.1"
                     /translation="MGVISLSPDGDSPIIEWAKPDVEGIFLAGFGLTPNLLARVNWNG
                     STPGSVEFRVNGTLHATLTGPGPEYHVSIPVDSALRTSLSATGNAIQVLARAQEVGMQ
                     SPPHKLTMMAVPVPQGLRTVVNSNLYRIFGPDHVGFDFELTKHEQKVTLPVVGTFGFE
                     WGANASFDYSIREGEWEAALGLGAEGKKGKRGRRPAFPGLIRQPKAKLYIGNKEISAS
                     LDGLASGLAKPSTGIVLQNVGVSAGISTRLELTRYGLLDLFGPGLTNAFSTIPVVNSV
                     LKNVSVIVWLIPGLEGSATAKVSPFSFDTATLTGRIGVEGAYEPEVGSVLKGRVYLGT
                     DGAATFKLPAPVFQNVSFRLYGGYEFSAWVLKVSKEVVFVSYTYPSGVSAFGAPGAMD
                     IGTGYRLEAAGNQRATWRAMDRPWRTKGKEVFLQGGEGNLRSLEGGMASPELELFARM
                     GNLDAAGENRRGEGGIGVMVVDSDLPAQTELPLLGNVFPDSEPALASQGQNLMLLYVR
                     DTGAPNPIHFTEAAWTFFDGTAWTTPVPVAGDVRGQFEPAVSFDGQGAAVAVWTRMKN
                     AGFASEDLAEMAAQMEMVAARWDPVAKTWNAPVALTDNAFLDHKPRLAGPLTNGDLVL
                     TWRENAGNQLIGSAAQTTRIMTRRWNAAAGTWGVPQELVPGLAHEFSVSLAARAGKAV
                     LVLTRTMTGAEDLSDTELFFKIWDEGAGAWGPLTRHTTDAVADRNAQAAIGPNGDIYC
                     VWQRGDDLVMDVDFSGNPAVVRPDSGTFGFSDLAVTVGPDGNVVVLWQEMSQHGSDAQ
                     YRVFDPASVTWGKDAFLSQDADLERSFAPVWDAAGNLTLAYNNVEIVKKTKSVVVEGG
                     EVVEVEGVPQPGRVDLLLAKRQLVKDVTLVPEGLTADGTNFLPGDEVTLRARVRNAGN
                     LAVQDLQVAFYDGDPDAGGVLSATEVIPGWLEAQDEVEVSHAWMVPQPVQARTVFVVV
                     DPDNEVTEADESNNRLALPLNGVDLALEYQSGSALRDGSARVVVKVRNLSAPDSPVSM
                     LKLKDLNSGVTLAETSVSQLAPGQSVDVPFDLPAGTQPEGDRAYVLVIDEEELVEDID
                     RENNEAPFALNLWIDDDGDGIPRWWELANGTSDDNEGDALLDLDGDGFNALQEFLAGT
                     DPADRNSRLALGGQNVVEHVNGDPGTHSISWPSAAGRLYRLERSYDLEAWETVEENIE
                     ASPPLNQKLDTPGMTGKRVFYRVTVQ"
     gene            10957..>11362
                     /locus_tag="PLP58_15940"
     CDS             10957..>11362
                     /locus_tag="PLP58_15940"
                     /inference="COORDINATES: protein motif:HMM:NF019262.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ10071.1"
                     /translation="MDFSREVLPVLQRACFECHGAEEARGGLRLHEKAAISAGGKHGA
                     VITAGKPDESELLRRVSLPRTDKQAMPRRGAPLSKDEIAKLREWIAAGAVWPETVTLQ
                     QHWAYVPPVKPAVPQGAAHPLDAFTRARLAAEG"
BASE COUNT         2088 a         3352 c         3840 g         2082 t
ORIGIN      
        1 caggcccgcg cggccaagag ccttccagtt ttccaccgcc tggtcggcgc gcgcctgctc
       61 ctcggccagc agggcttccg cctgggcctg cgccgcctgg gcgatcacga ccgccgtctc
      121 gtagtccagc gggtccagcc gcagcagcac ctcgtctttt tcaaaaaagc cgccggggcg
      181 gaaggccggg ctgacctcga cgatcttgcc cgccacctcc ggcagcaggc tgctgcgcgt
      241 gcgcgggcgc accgtgccct gggagcgcac gcgcggctca tagagtccct tgcgcagcac
      301 cgtgccctcc acgcggacga gcggcgcggg cacggatttt ttttcctgct ctggcggatg
      361 cgccagcagc caccacgccg catacccgca gccgccgagg atcagcaagg gaacggtgaa
      421 gcgcaaaatg gagcggagat aattcattcg gacaggagtt taaacgcgcg ggacagcctt
      481 tggattccag actttcggac gccatcagcc gccgttttgg tactggagtt ggtagagttt
      541 ttgataaaga gggctggtct gaagcagcgt ctgatgcggc cccaccgcct ccacccggcc
      601 ctcgttcatg acgatgatct ggtcggcttc cagaatggtg gagagacggt gcgcgatggc
      661 gatgaccgtg cgcccctgcg ccagggtctc gatggcctcc tggatgattt tctcggtctc
      721 ggtgtcgagc gcggaagtgg cctcgtcgag cagcaggacg ggcgcgttgc gcaaaatggc
      781 gcgggcgatc gagatgcgct gcttctggcc gccggagagg ttgcagccgg catcgccgac
      841 cacggcgtcg tagccgccct ggatttgctg gatgaactcg tgcgcgtgcg cctgcttcga
      901 ggcggcgagg atttcatcct ccttggcatc gagacggccg taacggatgt tttcgcggat
      961 ggtgtcgtgg aagagaaagg tgtcctggct caccatgccg atgttttccc gcaggctgct
     1021 taccgtggcg agacggatgt cctccccgtc cagcagcacg cggccggagt ctggatcata
     1081 gaagcgcagc aaaagagaga aaagagtgct cttgcccgcc ccgctggggc cgaccagggc
     1141 gtaaaatctg cccggctgga gatccaggct cacgccgcgc acggcggctt tttccagctt
     1201 cgcgccggac ggcgcgtggt aggcgaagga cacttcgtca aaacggatcg ctccagaaac
     1261 gcgcggcagc ctcctcgcat ccggcgcgtc ggtgatgtcc ggctggcggt cgagcacgcg
     1321 gaagatgttg ttgatggcat agaccgtctt ttgcatgagc aggctcacct tgctcagctc
     1381 cttggcgggc gggtaaatct tcgagagcgc cagcacgagc acgatcagct catgcgcggg
     1441 cctctccttc caccaaaagt aaaccagccc ggccgcgatg ccgatggagg ccacggcctc
     1501 gacgatgggg ccgatcatct ccgtcgcgcg cgtccagcgc aggatgttgc gcgtcatctc
     1561 gcggttggcg cggtcgaagc gtttgcattc gtagtcctcg cgcgcgttgg ccttcaccag
     1621 gcggatgccg gtgaaggact cgtgcatggc cgtcatcatg gcgccgacct ccttttcctc
     1681 gcgcgcgccc accttgcgca cgcggcgccc gatgacaagg atcggcgcca ggcagagagg
     1741 gaagaccacc agcgacatga gcgtgaagaa ccagtccatg gcgaaaagcg tggccaggat
     1801 cacgatgatg gtcagcggcc ggttcgagat gagctgcacc acctgcagcg cgtgactctg
     1861 cgccacgcgg gattgatgcg agaccacctg catgagctcg cccgccttgg tggtggcaaa
     1921 gaacgccggc gactggcgca tgatggccgc gaagacatcg ctgcggattt ggaagagcat
     1981 cttcgtgccc acccaggcca ggcagtattt gttcagatac tccaggactc cgctcagaaa
     2041 catcagcagc ggaatgcagg cgcaggccgc gatgacgccc caaaggccca tcggcgtgcc
     2101 gggctccagg ccgagcatct caccgaggtt gatcttgccc acgaaggcca tctccacggg
     2161 catgctcagg tccttgctcg cgccgtcgag cacgagttga aaaatcaact gcaaacccag
     2221 gatcatcacc gcgttgaatg acgccgccag cgcgcccagc cccatgccca gaaagaacct
     2281 gcccagccag ggccgcaagt agggcagcag acgccggtag ggcattttca ggtgcttgag
     2341 ctcggcttcg gtgatgggag ccaggctgtt ggagtgcttc gctgcggaca tggatcaatg
     2401 aaaaagcggg catcggcccg ctgccgacac ttagcattgc ggcatttccg cgtcgagcca
     2461 gttctgtgcc cggcaacaag ccaccccggt tctttccggt ttcatttact tttgtggaaa
     2521 caagccgaca tccatcctcc accgtgcttt ttgactcgca caaccacctt catgacgcgc
     2581 gcctgctgcc gtggcgcgcg gagatcatgg cagagctgcc cgcgctgggc attgccggtg
     2641 tggtggccaa tggcacctgc gagcaggact ggcccgccgt ggcggaactg gcgcgggcgc
     2701 atccctgggt gcggccctcc tttggcctgc atccctggcg ggtcagcggg cgcggtcccg
     2761 agtggaagga aacgctggag gcatttctgg cggagtttcc aggcgccgca gtcggggaga
     2821 tcgggctgga ccgctggatg gagcagcctg atgtcgaagc gcagcgcgag tgcttactct
     2881 ggcagctcga tctggcggtc cgtttggagc gccctgtcac agtccattgc ctgcgcgcct
     2941 gggggctgct ggatgaggtg ttgcgcgcgg cgcgcctgcc tgagcggggt tttcttttgc
     3001 actcctacgg aggtccggcg gaaatgatcc ccggctttgt gaaaatgggc gcgtttttct
     3061 cctgctcgcc ctggttcggt catccggcca aatccgcgaa actggcggtc ttcaagagtg
     3121 tgcccgccga ccgccttttg atcgaaaccg acgcgccgga catggcgccg ccggaggaac
     3181 tcgacacaca tccgctgtgg cacgaaggcc tgaggctgaa ccacccggcc aacctgcgcg
     3241 tgagtctctc tttgctgtca cgcctgcgcg gaacgcctgg ggatgagctt gcggcgcaga
     3301 tcgcggccaa tcaccggagt ctctttggcg aatgcggggt cactcgggat gcacgatgac
     3361 aaaagtgcgg cgcgtctcgc ctgtggccgg atcctccgcg cgcaacgtgt ggcgccccgg
     3421 cttgagcagg gccacgggtt ggccggcgtc gtccacgatc tccagcgtgc cgcaatccca
     3481 ccgcactccc tgctgattcg cctggagcag cagacgcgcg ccggagccgg gcaggtcggg
     3541 atccagatgt atgacggtgc cgggaactgg attggtgatc tgccaggggc gcttctcttt
     3601 tttcgccgtc tcgcacacga cgaggtcgcc cagccagttg tccgcgccgg ccacccagtc
     3661 cgcgtagatg cctggcagga tggcccggcc ctgcgcgtcg tagtcctccg cgccggccgg
     3721 gggcggcagg cgtcgcggag ggaaaaaatc ctcgcggctc agacgcgcgg gcggcgactg
     3781 cggtgtgaga cgcctgccgt tgcgcggatc cacgcgcgcc ttcacgagac cgggcggcgg
     3841 ttcatgccag gagacaccgc gccggcgctg caactccagc atcacgtccc gccagatggg
     3901 ggccgcgccg gtcactccgc tcacgtcctg catgggccgg ttgtcgaaat tgcccgccca
     3961 cacgccgacg gtgaactcgc gggtgaagcc gagcgtccag ttgtcgcggt aggtggagct
     4021 ggtgccagtc ttcacagcgg cggggaaagg cagccgcagc acggagttct ggccgaaggt
     4081 cagcgcgcgc gccgagttgt cggcgagaat gtccgcgatg agataacaaa gagtgccgtc
     4141 aagcagccgc gcgccctcgt tttcccgcgg cagcagcgac cagggtttgt gaatgcccag
     4201 gcgcgccagg caggcatagg cgttcgccag ctccacaagc cgaacgggcg cgttgccgat
     4261 cgtgagtccg ggaccgtaat gctccgcgct ttccgtgaga gtggtcagcc ccagctcccg
     4321 cagcctgtgc agcaactccg ccgcgcctcc ggcggactga agcactttca cagccgagac
     4381 attcagtgag tttcccagcg cggctcggta tgtcatcggg ccgtaatgct tgagcgtgta
     4441 gttttccggc aggtaggagc cgctgccggt ggagtactcc actggcaggt ccgccacgat
     4501 gctcgccggt gtcgcgccgc gctcaaaggc caggagataa gtgaagggct tcaccgccga
     4561 gccgggagaa cggggagtcc aagcgccgtt gatctggccg ccgtccggcg cggaaaaatc
     4621 gcgcgaaccc gccagcgcaa ggatgtggcc ggtggcgttt tcaatgacca ccaccgccgc
     4681 ctgggtgacc tggcgctccc gcagtccgga gagtctttcc gtgatgacgc gctccaccgc
     4741 gcgctgcagc gccgcgtcca gtgtggtgag caccactgga tccggcgccg ttccgccggc
     4801 gagcatttgc accgcgtgcg gcgcctcgaa gcctccgttg aagcgccgca gcctgacggg
     4861 ttcggccagc gcggtttgca actgccccgc gtccagccag ccgagcgcgc gcattttctc
     4921 caggatgcgc gtctggcgcg gccgggcgcg ttcgatgccg cgaaaagggt tcagccgcgc
     4981 gggcgactgg gggatggccg ccagcagcgc gcactctgcg ggtgagagat caggcagcgg
     5041 tttgtcaaag tagcccagcg ccgcgctctc gcagcctgtc agcaggttgc cgtaagagac
     5101 gcggttcagc caggctgcca gcacctcctc cttgctccag gtcatcgcca gacggcgcgc
     5161 ttggagcgcc tccaccagct tcacgccaag ggttcgcgga cctggccggg cggcggtgat
     5221 tttgatgagc tgctggtgca gcgtggaggc tccggagacc acccttccgg cgcgcagatt
     5281 gtcccaggcc gaacgggcga tcgccagcaa atccacgccg ccatgctgaa agaagcgctt
     5341 gtcctcagcc gcaagcaccg catttcgcaa atgctccggg atgtccgcat aggaagccgt
     5401 gcctgccacc cgctgtccct cctcagtcag gagcatgcgc agcggtgtgc catcggcggc
     5461 cagaaagcgc gtcgagacac tctgcgccgc aagcaaggcg ggcggcaaag gcgccagcca
     5521 cggcgcgcca aaccacgccg ccagcgcgcc cgcgagcagg gcggcggaga agacagtcag
     5581 gcggcggcgc atgggccaga taacgccgga aagcccggct tctccagccc cgttctcagc
     5641 gcgctcgcag aggaagtcgt cagacttccc tgactggaca gcaaaaagcg cgcgtgagtt
     5701 tgagtgcgcc cactcccctg gaaaacctct acgtgtcaag gcagagcgcg ggcgcccata
     5761 gaggaagtcg tgagacttcc ccggcgcttg agaagcacct ggaacgatgc cggccagctt
     5821 ggcggctcaa gccgctcacg cttagggaaa ggaaacccgt ccatccccga acctggattc
     5881 ctgggttgca ttaccctcgg aaaatcccat tacttcgcgt catgtatcga ggatttccac
     5941 tttttctagg cttgcttctc gcggctctct gcatgggccg ggccggggcg cagaccgtga
     6001 cgaatgtgcg cgctgcgcag cgggcgggga cgaagctggt ggatgtgtat tatgacctat
     6061 ctggagttca gggtctcact aagatggaag tgggagtctc aactgacggc gggatcagct
     6121 acgcgacctt aaactcagct caattgaccg gggatgttgg cggatggaca gcagacggaa
     6181 acaacaagca tttcgtttgg aatgcaggca ttggttggaa tgaactaaaa tcggatttag
     6241 taagatttag gatccgcaca tcctctctcg aatttactgt gacggttacc acccttgaat
     6301 tcgatcctga tcatccgatt gaggtcgaag taatcgatcc cgtcgatgga aatcccgtca
     6361 aagtcaccat gtattgcggg gattactccg ttggagaaac tagaattgta tcagactcct
     6421 gggtgtttga cggaagtgat ggaaccatga ccatctcgtt tgatgatggt tcaataaatg
     6481 gaacattcga tagatcaact gggatcttcg aacttgatta cgctgtccca agggaaactg
     6541 ttcttgtgac tcccttggtc acttattttt cagaatcaag cggccatata gatggtgtat
     6601 tcgacgtgca gaacattaga tttacagggg aggaagtgat agaggaagcg acaagctggt
     6661 ccattgatgc gagcagggtg ggttgtttgg tgagagaggg tattgttgga gagggggagt
     6721 ggggttcagc ttcagagatt gtatcaccaa taatttcagt agacacgagc ggcgagggtg
     6781 atgtcacggt tatgggacag gttttgaatg ggcagaacaa tctgccattg ccgggagcgc
     6841 tagtcgtgct tgcagggcag agcacttcca ctgccgccaa cggcaccttt aaacttgtaa
     6901 acgtaaatct ggcagatgaa ttgaccctga cagctggcgc atccggcttc gcctcccaat
     6961 cccgcttggt tcaggcggca gtaggtacat caagtgtgga tgtgggggtc atctcactct
     7021 caccggatgg cgactcgccc atcattgagt gggccaagcc ggatgtggag ggcattttcc
     7081 ttgcgggctt tggccttacc cccaatctcc tggccagggt gaactggaac ggctcgactc
     7141 ccggcagtgt agaattccgg gtgaatggga cgcttcatgc cacgctcaca ggaccaggac
     7201 cggaatacca tgtgtccatc cccgtggaca gcgccctgcg cacctcgctc tccgccacgg
     7261 gcaatgccat tcaagtcctc gcacgcgccc aggaggtggg catgcagtca cctccgcaca
     7321 agctcaccat gatggcggtt cccgtgccgc agggtttgcg cacggtggtg aattcgaatc
     7381 tgtatcgcat tttcggaccc gatcatgtgg ggtttgattt cgaactgacg aagcatgagc
     7441 agaaagtgac actgcctgtg gtaggcacct tcggctttga gtggggagcg aacgccagct
     7501 tcgactacag catcagggaa ggggagtggg aagccgccct tgggctggga gccgagggaa
     7561 agaaggggaa acgggggcga cgtccggctt ttcctggcct gatccgccaa ccgaaagcca
     7621 agctctatat tggcaacaag gagattagcg cttctttgga tgggttagcg tctggcctgg
     7681 ccaaaccgag caccgggatc gtgctgcaga atgtcggagt gagtgccggg atctcgaccc
     7741 ggctggaact gacccgctac ggcctcctgg atctcttcgg accgggtctg acaaacgcgt
     7801 tctcaacgat tcccgtagtg aacagtgttt taaagaatgt gtcggtcatc gtgtggttaa
     7861 tccctggcct ggaggggagc gctacggcga aagtgtctcc cttctccttt gacaccgcga
     7921 cgcttaccgg tcgcattggg gtggaggggg cctatgaacc ggaggtgggg agcgtgttga
     7981 agggcagagt gtacttgggc acggacggag ctgcgacttt caagctgccc gcgcccgtgt
     8041 ttcaaaacgt cagcttccgg ctctatggcg gctatgagtt cagcgcctgg gtgctgaagg
     8101 tgagcaagga ggtggtcttc gtgagctaca cttacccgtc gggagtctcg gccttcggcg
     8161 ctccgggtgc gatggacatc ggtacgggtt acaggctgga ggcggcggga aatcaacgcg
     8221 ccacctggcg ggccatggac cggccctggc ggacgaaggg caaggaggtg tttcttcagg
     8281 gcggggaggg aaacctgcga agtctggagg gtggcatggc atcgcccgaa ctggaacttt
     8341 ttgctcgaat gggaaatctg gacgcggctg gggagaaccg tcgtggtgaa ggcggcatcg
     8401 gcgtgatggt ggtggactcg gacctgcccg cgcagacgga actgccgctg ctggggaatg
     8461 tgttccccga ctcggaaccg gctctggcaa gccaagggca aaacctgatg ctgctctatg
     8521 tgcgcgacac gggcgctccg aacccgatcc atttcaccga ggcggcctgg acgttttttg
     8581 atggcacggc gtggacgacg cccgtgccag tggcagggga cgtgcgcggt cagtttgaac
     8641 cagcggtgtc gtttgatgga caaggcgcgg cggtggcggt ctggacccgg atgaagaacg
     8701 cgggatttgc cagcgaggat ttggccgaaa tggcggcgca gatggagatg gtggccgcgc
     8761 gctgggaccc ggtggcgaag acctggaacg caccggtggc actgacggac aacgccttcc
     8821 tggatcacaa accgcgtctg gccggtccgc tgacgaatgg ggacctcgtc ctgacctggc
     8881 gggagaatgc cgggaaccaa ctgatcggca gcgcggcgca gaccacgcgg atcatgacgc
     8941 ggcgctggaa cgcggcggca ggaacctggg gcgtgccgca ggagttggtg cctggactgg
     9001 ctcatgagtt ctcggtttcc ctggcggcca gggctggcaa ggcggtgctg gtgctgacac
     9061 ggacgatgac gggggcggag gatttgagtg acacagagct gtttttcaaa atctgggatg
     9121 agggagcggg agcctggggg ccgttgaccc ggcacaccac ggatgccgtg gcagaccgca
     9181 acgcgcaggc ggccatcggc ccaaatgggg acatctactg tgtctggcag cgcggagacg
     9241 acctggtgat ggacgtggat ttctcgggga atccggcggt ggtgcggcca gactcgggga
     9301 cttttggatt ctcagacctg gcagtcactg tggggccgga tggcaatgta gtggtgctct
     9361 ggcaggagat gagccagcat ggctcggacg cgcaataccg cgtctttgat cccgcatcgg
     9421 tcacctgggg gaaagatgcg tttctctccc aggatgcgga cctggaacgc tcgttcgcgc
     9481 cggtgtggga tgcggcgggc aatctgacgc tggcttacaa caatgtggag atcgtgaaga
     9541 aaaccaaaag cgtggtggtg gaaggcggag aggtggtcga ggtggagggc gtgccgcagc
     9601 cgggccgggt ggacctgctg ctggccaagc ggcaactggt gaaggatgtg acgctggtgc
     9661 cggagggcct gacggcggat ggcacgaatt tcctgccggg cgacgaggtg acgctgcgcg
     9721 ccagggtgcg gaacgcgggc aatctggcgg tccaggattt gcaggtggcc ttttatgacg
     9781 gcgacccgga cgcgggcggg gtgctcagtg ccacggaggt gatccccggc tggctggagg
     9841 cgcaggatga ggtggaggtc agtcacgcct ggatggtgcc ccagcctgtg caggcgcgga
     9901 cggtctttgt cgtggtggac ccggacaacg aggtgacgga ggcggacgag agcaacaacc
     9961 ggctggccct gcccctcaat ggcgtggact tggcgctgga gtatcagtcc ggctccgcgc
    10021 tgcgggatgg ctcggcgcgc gtggtggtga aggtgcggaa cctgtccgcg ccggattcgc
    10081 cggtgagcat gctcaagctc aaagatctga acagtggtgt gacgctggcg gaaacgagcg
    10141 tgagccaact cgcgcccggc cagtcggtgg atgtgccctt tgacctgcct gcgggcacgc
    10201 aaccggaggg agaccgcgcc tatgtgctgg tgattgatga ggaggaactg gtggaggaca
    10261 ttgaccgcga gaacaatgag gcgcccttcg cgctgaatct gtggattgac gacgatggcg
    10321 acggcatccc gcgctggtgg gagctggcga acggcacgag cgatgacaat gagggagacg
    10381 ccctgctcga tctggacggc gacggcttca acgcattgca ggagttcctg gccggaaccg
    10441 accctgcgga tcggaactcc cgcctcgccc tcggcggtca aaatgtggtg gagcatgtga
    10501 atggcgatcc cggcacgcac tcgatctcct ggccttcggc agcaggcagg ctgtatcgcc
    10561 tggagcgctc gtatgatctg gaggcctggg aaacggtgga ggagaacatc gaggccagcc
    10621 caccgctcaa ccaaaagctg gacactcccg gcatgaccgg gaagcgcgtg ttttaccgtg
    10681 tcacggtgca gtgagaaaat cgagttggaa tagccgcaaa gaggcgcaaa aaacgcaaaa
    10741 caagggggag cagcgccttt cattttgaga gtgcgcgcac gacgaacctg ggtctctaat
    10801 tttttgcggc tttttgagct ttttgcggcc attcattccc tgctgatgca gccggcccgt
    10861 caacaccacc ggaaagcccg gcttctccag ccccgttctc agcgcgtgaa aagggttctg
    10921 acattttttc ttctgtgcgg cgccgccacg catgccgtgg atttttctcg cgaggtgctg
    10981 ccggtgctgc agcgcgcgtg cttcgagtgc catggcgcgg aagaggcgcg cggaggattg
    11041 cgcctgcatg aaaaggccgc catttccgcc ggaggcaagc acggcgcggt gatcacagcg
    11101 ggcaaacccg atgaaagcga gctgctgcgc cgcgtgtccc tgccgcgcac ggacaagcag
    11161 gccatgccgc gccggggcgc gccgctgtcg aaggacgaga tcgccaaact gcgcgagtgg
    11221 atcgccgccg gggccgtgtg gccggagacg gtgactttgc agcagcactg ggcctatgtg
    11281 ccgcccgtga aacccgccgt gccccagggc gcggcgcatc cgctggatgc cttcacgcgc
    11341 gcgcgtctcg ccgccgaggg cc
//
LOCUS       DAOMYJ010000172        11340 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Prosthecobacter sp. isolate AMR_MDS_5629
            NODE_1902_length_11340_cov_20.039344, whole genome shotgun
            sequence.
ACCESSION   DAOMYJ010000172 DAOMYJ010000000
VERSION     DAOMYJ010000172.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867850
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prosthecobacter sp. (wastewater metagenome)
  ORGANISM  Prosthecobacter sp.
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Verrucomicrobiaceae; Prosthecobacter.
REFERENCE   1  (bases 1 to 11340)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11340)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.30
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:34:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,742
            CDSs (total)                      :: 4,684
            Genes (coding)                    :: 4,676
            CDSs (with protein)               :: 4,676
            Genes (RNA)                       :: 58
            tRNAs                             :: 55
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11340
                     /organism="Prosthecobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1902_length_11340_cov_20.039344"
                     /isolate="AMR_MDS_5629"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1965333"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..441
                     /locus_tag="PLP58_15945"
     CDS             <1..441
                     /locus_tag="PLP58_15945"
                     /inference="COORDINATES: protein motif:HMM:NF019474.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HRJ10072.1"
                     /translation="GGGSAGGHLAAALATLDEVNDPDDDLSIPLKPAALVLFNPALVL
                     ESPRAAEAARLDSVASLRAYSPYSHVKKGHPPALILHGEADTTVPIATARQYAARVVE
                     LGGQCEVVGYEGAPHSFFNRSPHFEQTSARMEQFLQSLGWLKRE"
     gene            495..2009
                     /locus_tag="PLP58_15950"
     CDS             495..2009
                     /locus_tag="PLP58_15950"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase"
                     /protein_id="HRJ10073.1"
                     /translation="MDSEIRGNPFHPWFTSISMRRLCLALTLLLASSAFARPLNILWL
                     VGENLCHDLGCYGAAEARTPNLDALAARGMRFTQVIATNPACAPSRSAFFTGMYQTST
                     DTHPMRSHRDDDFRLPPGVRPVTHWLRDAGWFTANLKTMGDLQIGTGKLDLNFVNEGP
                     IYHPGSEDWAALPKDRPFFAVVNSHENEYDIYDRQTWKHERAEWVGEKDPAFASPASP
                     ENMTPPPYYPDHPIVREEWARYWNSIHRMDHRIGWVLDRLRADGLEDDTLVIFFGDNG
                     RIEPRGIHWCHDSGLRVPMILAWPKNHPAPPQYQPGGVSDQLISLLDLTATTLAAAGV
                     PRPPLMQSRVFLGTDADPPRAYAFAARDRIDETEQRIRSVHDRRFHYIRTFSEGPTFA
                     SLNRYKEKCFPILPLMRRLHAGGSLTGPPLELMERRGPCEELYDLQNDPHEIQNLLTS
                     EKPEHREALARLRAALDTWIADTGDRGAKPEPRQVVAPFAKEMHDWFGTPDWVR"
     gene            2196..3608
                     /locus_tag="PLP58_15955"
     CDS             2196..3608
                     /locus_tag="PLP58_15955"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HRJ10074.1"
                     /translation="MPRLLSLLAALVVPALAADKPNILFIFADDQCFETIRAFGHEDI
                     DTPNLDRLVTGGTTFTHAYNMGSWSGAVCVASRTMMATGRGLWDARAIYDSTDKEREA
                     GRLWPQLMAAQGYATYYTGKWHVKADAAKAYEFTSNIRAGMPKDTPEGYNRPLAGQPD
                     AWSPSDPKWGGFWQGGKHWSEVVGDDAVAFLAQAAKDTRPFFITAAFNAPHDPRQSPK
                     EYVERYPLERVKVPENYLPEYPHKDAIGCGPGLRDEKLGPFPRTEHAVKVHRQEYYAI
                     ITHMDAQIGRILDALERSGKAGQTYVFFTADHGLAVGHHGLFGKQNMYEHSLRVPLLV
                     RGPGIPAKAKVSAPVHLQDIMPSALELAGAPKPPHVFFHSLLPLARGQTQSSAYPVIY
                     GAYLELQRALIHDGWKLIVYPKAGVMRLYHLKEDPQEMRDLAADPAHTEKKRALFTRL
                     IERQKELSDTLDLRAAFPNG"
     gene            complement(3621..4823)
                     /locus_tag="PLP58_15960"
     CDS             complement(3621..4823)
                     /locus_tag="PLP58_15960"
                     /inference="COORDINATES: protein motif:HMM:NF024120.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HRJ10075.1"
                     /translation="MRALFLFIAIVSTAAAQETPVTVNAGRHANLQEAFDALPAAGGI
                     VLIPPGEYRIQKPLHVSTAETRIVGSGAATHIINENTEGRPAIILRPADLDTNKQARL
                     WRVQLADFRVSGQEKSGDGIFAEGVQEIYFEGMSIDHHGGNGIHMVNCTEDPRVADCI
                     LTYNKAAGIVINGGHDIVVSANHFEENLDALHCFDAFNLCMTGNNIDDHLRHGVVIEN
                     TYGSVVSGNMIEECNGTAIILDRDCYGITLSANVIAHHLEGGMDLRDAHGCSVSANTF
                     TILHKFSVRVGPESGRLAITGNSFCNTWIGGQDKRPAEGKTPMSIDEGTGVLLEAGAR
                     HVTLTGNSFTGLSGAAVWTTGECRSILVSANVLTDCGRKLGKDAPWLDLASVKNGVVK
                     DNIEDRGE"
     gene            complement(4894..5727)
                     /locus_tag="PLP58_15965"
     CDS             complement(4894..5727)
                     /locus_tag="PLP58_15965"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF024545.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HRJ10076.1"
                     /translation="MKTLLFSLALLAALLALRAWRAERKPAAWDPLPALAWRHGGESQ
                     AARAGAAFHEQVRALRLADTSFQHQADTAPEMAVWRRQFQSEEERLHRLTCAGLDEAA
                     FESDLREHLLDEAWIEHHLARAASAVDEAAARAWHQKHRERLRIPEAHRAAHIFLSRH
                     GGKSDRLAEITALRQRLQNGESWETLASAHSEDARTRARGGDLGWFTAARMPAEFMLA
                     LQSLTPGQTSAPVQTRLGWHLIRLQARRPARVPAFEEVRQEILAALQSEARVRALEGL
                     R"
     gene            complement(5724..6779)
                     /locus_tag="PLP58_15970"
     CDS             complement(5724..6779)
                     /locus_tag="PLP58_15970"
                     /inference="COORDINATES: protein motif:HMM:NF021535.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HRJ10077.1"
                     /translation="MMCDGNWRCALLPLLAAGALASCSTAGFYRQALAGQMEMSRKAR
                     PVADVLADPASKPLLREKLTTARDILAFAEAELGLPAKGQYERYANLGRRYSVWVVFA
                     APEFSVEPKRWWYPLIGSLKYRGYFQEAPALREAERCRAQGLETFVGGVEAYSTLGWL
                     RDPLLNTFIGRDDGDLAELLFHELTHQRLYLPGDTDFNEALATAVGQEGARRWLKARG
                     RPDDLRAYEKELRVERQFIAELLRARDELKTLYARADMEENALRQAKQAVFDQLRARI
                     RALDQRHGGSLKLEKFFAKPVNNARLATVATYHDLLPDFEALLKRCDGDLERFFKEVR
                     ALRHLKPEERAQRLRSP"
     gene            6789..8240
                     /gene="uxaC"
                     /locus_tag="PLP58_15975"
     CDS             6789..8240
                     /gene="uxaC"
                     /locus_tag="PLP58_15975"
                     /EC_number="5.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009962073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008880 - glucuronate isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006064 - glucuronate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HRJ10078.1"
                     /translation="MNGIRANLKFSSAMLFIHDDFILSTQTARRLYHSYAKDEPILDY
                     HNHLPPKDIAEDRQFKNLFEIWLEGDHYKWRAMRCNGVPEHLITGDATPKEKFLAWAA
                     TVPHTLRNPLYHWTHLELKRYFGIDELLSEKTAESIWERTEAALARPEMSARGILRAQ
                     KVRALCTTDDPADDLVWHRKCAQDGFEVGVYPTFRPDKAFAIDNLEAWNAWVDRLESA
                     SGKSIRKFQDLLDALAARHQAFHDIGCRLSDHGLNHCHASFVPRIRLETLFERLRNNR
                     SKRIYPEDREMLGTAVMLHVGEMNADKGWTMQLHLGPLRNNNSRLARLVGADVGCDSI
                     GDYPQAQALSRFLDALDSQGKLPKTVLYNVNPADNYVFGTMAGNFSDGVTPGKVQFGS
                     GWWFVDQKEGMEWQINALSNLGLLSRFIGMLTDSRSFMSFPRHEYFRRTLCNLLGRDV
                     EEGVIPDDDELTGAMIRNICYANAKAYLGLEKS"
     gene            complement(8340..10505)
                     /locus_tag="PLP58_15980"
     CDS             complement(8340..10505)
                     /locus_tag="PLP58_15980"
                     /inference="COORDINATES: protein motif:HMM:NF015168.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HRJ10079.1"
                     /translation="MNDPQKTPPKHTNALAREKSPYLLQHAHNPVDWLPWGDEAIAKA
                     RAEDKPIFLSSGYSTCHWCHVMERESFENEEIAAYINEHFVPVKVDREERPDVDLTYM
                     TYVQAASGGGGWPMTVFLTPDLKPFFGGTYYPPENAQGRIGFKNLLKELHKAWTEDRE
                     KVLAGAERSVSKLQEFLDGENQGREVAADAVLQKAYDELSGGYDYHEGGFSGAPKFPR
                     STSINLLLRIHLAWAKSAERKSESDWAGEMCARTLRAMANGGIWDHVGGGFHRYSVDG
                     YWHIPHYEKMLYDQGQLLWAYAEGHLATGSVFLAGVAREIAAYCARDMRHPDGGFFSA
                     EDADSLRSADEPHKHEGAFYVWKAAEIDDLLGRERGGIFRYAYGARRDGNARPESDPH
                     GELAGWNTLFKAFSAKKTAEYFKKPVEEVQTILADGLRLLFDARAKRPRPHLDDKIIT
                     AWNGLMIAGLARAGAALGEKSFLDMAAQAASFLHDQLCDEPGKGLHRSWREGGRGPRA
                     FAADYACLIHGLLDLYQSGFDSRWLRWAAALQEEMDALFLDAEKGGYYSVHTDMAHSV
                     LRIKEDYDGAEPSPNSLAALNLARLAAMLDHAPWLEQARKILALFGGTLDGSPSSVPV
                     LVMAADFIERGKQQIVLAGDSSSAEFQALAGVVHKRLLPHAVVLHAGGGEAQEWLGAR
                     NAALAEMKPVSGKPAAYVCRNYTCQAPVTEASALEKLLG"
     gene            10627..>11340
                     /locus_tag="PLP58_15985"
     CDS             10627..>11340
                     /locus_tag="PLP58_15985"
                     /inference="COORDINATES: protein motif:HMM:NF013109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015116 - sulfate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SulP family inorganic anion transporter"
                     /protein_id="HRJ10080.1"
                     /translation="MSQPSQPSWWQLLRSLLRQGRAMVRGTLTDAPLDPLPLRKRLRG
                     YNLPKLRSDVKAGANVALLDIPQSMAYAAVAGLPLQFGAMCSAVAGTLGALFGSSRFT
                     VLGPTNATAFMIFSYFAADPHLDRAALMPLLVFMVGALLIIGAWLRVAELAQYISRAV
                     IVGYVTGAALLIIANQLPNVLGISLLVEMPDGGVFQPRTMPGILWQLARKIFETSLPG
                     LCLAAGTALVYWGARRLRPA"
BASE COUNT         1900 a         3987 c         3526 g         1927 t
ORIGIN      
        1 ggcggcggct ccgccggcgg gcatctcgcg gccgcgctcg ccacgctgga tgaggtcaac
       61 gaccccgatg acgatctctc catcccgctg aaacccgccg cgctggtgct cttcaatcct
      121 gccctggtgc tcgaaagccc gcgcgccgcc gaggccgcgc gtttggattc cgtggcctct
      181 ctgcgcgcct acagtcccta cagccatgtg aaaaaaggcc acccgcccgc gctcatcctg
      241 catggcgagg ccgacaccac cgtccccatc gccaccgccc gccaatacgc ggccagggtg
      301 gtggaactgg gcggccagtg cgaggtcgtc ggctacgaag gcgcgccgca ttccttcttc
      361 aaccgctccc cgcacttcga gcagaccagc gcccgcatgg agcagttcct gcaaagcctc
      421 ggctggctca aacgcgagtg acacgcggat tcgcctcccg tcttttaaac cacggatcac
      481 acgaatctcc acggatggac tctgaaatcc gtggcaatcc gttccatccg tggttcacct
      541 ccatctccat gcgccgtctt tgcctcgccc tcacattgct gctcgccagt tccgcttttg
      601 ccaggccgct gaacattctc tggctcgtcg gcgagaatct ctgccatgac ctcggctgct
      661 acggcgcggc ggaggcgcgc acgccgaatc tcgacgccct ggccgcgcgc gggatgcgct
      721 ttacccaggt catcgccacc aatcccgcct gcgcgcccag ccgctccgcc ttcttcaccg
      781 gcatgtacca gaccagcacg gacacgcacc cgatgcgcag ccatcgtgac gatgacttcc
      841 gcctgccgcc cggcgtgcgg cccgtcaccc actggctgcg ggacgcgggc tggttcaccg
      901 cgaatctcaa gacgatgggc gatctccaga tcggcaccgg caagctggac ctgaacttcg
      961 tcaacgaagg ccccatctac catcccggca gcgaggactg ggccgcgctg cccaaggacc
     1021 gccccttctt cgccgtggtg aactcccacg aaaacgaata cgacatttac gaccgccaga
     1081 cctggaagca cgagcgcgcc gaatgggtcg gtgaaaaaga ccccgccttt gccagcccgg
     1141 cctcgccgga aaacatgacc ccgccgcctt actaccccga ccatcccatc gtgcgcgagg
     1201 agtgggcgcg ctattggaac tccatccacc gcatggacca ccgcatcggc tgggtgctcg
     1261 accgcctgcg cgcggacggc ctggaggacg acacgctcgt cattttcttc ggcgacaacg
     1321 gccggatcga gccgcgcggc attcactggt gtcatgacag cggcctgcgc gtgcccatga
     1381 tcctcgcctg gccgaagaac caccccgcgc cgccgcaata ccagcccggc ggcgtcagcg
     1441 accagctcat cagcctgctc gacctcaccg ccaccacgct cgccgccgcc ggagtgccgc
     1501 gtccgccgct catgcaaagc cgcgtcttcc tcggaaccga cgccgacccg ccgcgcgcct
     1561 acgccttcgc cgcgcgcgac cgcattgacg agaccgagca gcgcatccgc tccgtgcatg
     1621 accggcgctt ccactacatc cgcaccttta gcgagggccc cacctttgcc tcgctgaacc
     1681 gctacaagga aaagtgcttc cccatcctgc cgctcatgcg ccgcctgcac gccgggggca
     1741 gcctcaccgg cccaccgctg gaactcatgg aacggcgcgg cccctgcgag gagctttacg
     1801 atttgcaaaa cgacccgcac gagatccaaa acctcctcac ctccgaaaag ccggagcacc
     1861 gcgaagcgct cgcccgcctg cgcgccgcgc tcgacacctg gatcgccgac accggcgacc
     1921 gcggcgcgaa gcccgagccg aggcaagtgg tcgcgccctt tgccaaggag atgcacgatt
     1981 ggttcggcac accggactgg gtgaggtgat tcgccccctt cacatcttaa tccgtctctt
     2041 aatctgtgtc ttaatccggt ttggattctg cgggattaag acaaggatta agacacggat
     2101 tacgagaggg gttgcacccg cacagcaccc tccgtggggt tgaaatgaaa catcccccgc
     2161 ccaggttcgt gtcattcatg atgccccgct ctctcatgcc cagacttctc tccctcctcg
     2221 ccgccctcgt cgtgcccgcg ctcgccgccg acaagccaaa catccttttc atcttcgccg
     2281 acgaccagtg ctttgagacc atccgcgcct tcggccatga ggacatagac acgccgaacc
     2341 tcgaccgcct cgtcactggc ggcaccacct tcacccatgc ctacaacatg ggctcttgga
     2401 gcggcgcggt gtgcgtggcc agccgcacca tgatggccac aggacgcggc ctctgggatg
     2461 cgcgcgccat ttatgacagc accgacaagg agcgcgaggc tggccgcctc tggccgcagc
     2521 tcatggccgc gcagggttac gccacctatt acaccggcaa atggcatgtg aaggccgacg
     2581 ccgcgaaggc ctatgagttc acatccaaca tccgcgccgg catgcccaag gacacacccg
     2641 agggctacaa ccgcccgctg gccggccagc ccgatgcgtg gagcccctcg gacccgaaat
     2701 ggggcggctt ctggcagggt gggaaacact ggagcgaggt cgtcggcgat gacgccgtgg
     2761 ccttcctcgc tcaggccgcc aaagacaccc gccccttctt catcaccgcc gccttcaatg
     2821 cgccgcatga tccccgacag tcgcccaagg aatatgtgga acgctacccg ctggagcgtg
     2881 tgaaggtgcc ggagaactac ctgcccgaat acccgcacaa ggacgccatc ggctgcgggc
     2941 cggggctgcg ggatgaaaag ctcggcccct tcccgcgcac ggagcacgcc gtgaaagtcc
     3001 accgccagga atactatgcc atcatcaccc acatggacgc gcagatcggg cgcatcctcg
     3061 acgcgctcga gcgcagtggc aaagccgggc agacctacgt cttcttcacc gccgaccacg
     3121 gcctcgccgt ggggcatcac ggcctgttcg gaaagcagaa catgtatgag cacagcctgc
     3181 gcgtgcccct gctcgtgcgc ggcccaggca tcccggccaa ggccaaagtc agcgcccccg
     3241 tgcatctcca ggacatcatg cccagcgccc tggagctggc cggtgcgcca aagccgccgc
     3301 acgtcttctt ccacagcctg ctgcccctgg cgcgcggcca gacacaaagc tccgcctacc
     3361 ccgtgattta cggcgcttat ctggagctcc agcgcgcgct cattcatgac ggttggaagc
     3421 tcatcgtcta tcccaaggct ggcgtcatgc gcctctacca cttgaaggaa gacccgcagg
     3481 aaatgcgcga cctggccgcc gatcccgccc acacggagaa aaagcgtgcg cttttcacca
     3541 gactgatcga gcgccagaag gaactcagcg acacgctgga tctgcgcgcc gcttttccga
     3601 atggatgagc cgaagccggc tcactccccg cggtcctcga tgttgtcttt gaccacgcca
     3661 tttttcacac tggcgagatc gagccagggc gcgtccttgc ccagcttgcg cccgcagtca
     3721 gtcagcacgt tcgcgctgac caggatcgag cggcattccc ccgtggtcca caccgccgcg
     3781 ccggagagtc ccgtgaagct gttccccgtc agcgtcacat gccgcgcgcc cgcctccagc
     3841 aatactccgg tgccctcgtc aatggacatc ggcgttttgc cctccgccgg gcgcttgtcc
     3901 tgcccgccga tccaggtgtt gcaaaaactg ttccccgtga tggcgagccg cccgctctcc
     3961 ggccccacgc gcacgctgaa cttgtgcagg atcgtgaacg tgttcgcgct caccgagcag
     4021 ccgtgcgcgt cccgcaggtc catgccgccc tccagatggt gagcgatcac gttcgcgctc
     4081 agcgtgatgc cgtagcagtc ccggtccagg atgatcgccg tgccgttgca ctcctcgatc
     4141 atgttcccgc tcaccaccga gccgtaggtg ttttcgatca ccacgccatg acgcaaatga
     4201 tcgtcaatat tgttgcccgt catgcacagg ttgaaggcgt cgaagcagtg cagcgcgtcc
     4261 aggttctcct cgaaatgatt cgcgctcacc acaatgtcgt gcccgccgtt gatgacaatg
     4321 cccgccgcct tgttgtaagt caggatgcag tccgccacgc gcgggtcctc cgtgcagttg
     4381 accatgtgga tgccgttgcc gccgtggtgg tcaatggaca tgccctcaaa gtaaatctcc
     4441 tggacgccct cggcgaagat gccgtcgccg ctcttttcct gcccgctgac gcggaagtcc
     4501 gcgagctgca cgcgccacag ccgcgcctgc ttgtttgtgt ccaggtccgc cgggcgcagg
     4561 atgatggcag gccggccctc cgtgttctca ttgatgatgt gcgtcgccgc cccgctgccc
     4621 acgatccgcg tctccgccgt gctcacatgc agcggctttt gaatccggta ttctcccggc
     4681 ggaatcagca caatgccgcc cgccgccggc aaagcatcga aagcctcctg gaggttcgcg
     4741 tgtctgccgg cgttcaccgt cacgggagtc tcctgggcgg cggcggtcga gacgattgcg
     4801 ataaagagaa aaagcgcgcg cataaggcgg agtcatacgc ggcgggaaga ccgatgttcg
     4861 aaattcgaaa acatcctcgc ccaatttcat gctttaccgc agcccctcca gcgctcggac
     4921 gcgcgcttcg gattgcaggg cggcgaggat ttcctggcgc acttcctcga aggcggggac
     4981 acgggccggg cgtctggctt gcaggcggat gaggtgccag ccgaggcggg tctgcacggg
     5041 ggcgctggtc tggccaggcg tcagggattg cagggcgagc atgaattccg caggcatgcg
     5101 cgcggcggtg aaccagccga ggtcgccgcc gcgggcgcgg gtgcgggcgt cctcgctatg
     5161 ggcggaggcg agtgtttccc aggactcgcc gttttgcagg cgctgacgca gggcggtgat
     5221 ctcggcgaga cggtcgcttt ttccgccatg ccggctgaga aaaatgtgcg cggcgcgatg
     5281 agcctctgga atgcgcaacc gctcgcgatg tttctgatgc caggcgcggg cggcggcctc
     5341 gtccacggcg gacgcggcgc gggcgaggtg atgctcgatc caggcttcat cgagaaggtg
     5401 ctcgcgcaag tccgactcga aggcggcttc atcgaggccg gcgcaggtga ggcggtgcag
     5461 gcgttcttcc tcgctctgga actgcctgcg ccagacggcc atctccgggg cggtgtccgc
     5521 ctggtgttgg aatgatgtgt cggccaggcg cagagcgcgg acttgttcat gaaaggcggc
     5581 tccggcgcgg gcggcctggc tttcgccgcc atgcctccag gcgagggcgg gcaggggatc
     5641 ccaggcggcg ggctttcgct cggcgcgcca ggcgcgcagg gcgagcaggg cggccaggag
     5701 cgcgaggctg aagagcagtg tcttcatggg ctgcgcagac gttgcgcgcg ctcctcgggc
     5761 ttcaagtggc ggagggcgcg gacttccttg aagaagcgct ccagatcgcc atcgcagcgc
     5821 ttcagcaggg cctcgaaatc cggcagcaga tcgtggtagg tggcgacggt ggcgaggcgg
     5881 gcgttgttga cgggtttggc gaagaacttc tccagcttca aggagccgcc gtggcgctgg
     5941 tcgagtgcgc ggatgcgggc gcggagctgg tcaaagacgg cctgcttggc ctggcgcagg
     6001 gcgttttcct ccatgtccgc gcgggcgtag agggtcttca attcatcgcg cgcgcgcagg
     6061 agttcggcga tgaactggcg ctcgacacgc agctcttttt cataggcgcg caggtcgtcc
     6121 gggcggccgc gcgccttgag ccagcggcgg gcgccctcct ggccgacggc ggttgccaga
     6181 gcctcgttga agtcggtgtc tccaggaagg tagaggcgct ggtgggtgag ttcgtggaag
     6241 agcagttccg caaggtcgcc gtcatcgcgc ccgatgaagg tgttcagcag cggatcgcgc
     6301 agccagccga gagtggagta ggcctcgacg ccgccgacga aggtttccag cccctgcgcg
     6361 cggcaccgct ccgcctcgcg caatgcgggc gcctcctgga agtagccccg gtatttcaaa
     6421 ctgccgatga gcggatacca ccagcgcttc ggctcgacgc tgaactcagg cgcggcaaag
     6481 accacccaca cggagtagcg gcggccgagg ttggcgtagc gctcgtactg gcctttggcg
     6541 ggcaggccga gttctgcttc ggcaaaggcg aggatgtcgc gcgctgtcgt cagcttttct
     6601 ctgagcaggg gtttgctggc ggggtcggcg agcacatcgg ccacgggccg cgccttgcgg
     6661 ctcatctcca tctggccggc cagcgcctgg cggtaaaagc ccgccgtcga gcacgaggcc
     6721 agcgcgcccg ccgccaggag cgggagcagg gcgcatctcc agttgccatc acacatcatc
     6781 cgcccaccat gaacggcatt cgcgccaacc tcaaattttc atccgccatg ctcttcattc
     6841 acgacgattt catcctctcg acccaaaccg cgcgcaggct ctaccacagt tacgcgaagg
     6901 acgagcccat cctcgactac cacaaccacc tgccgccgaa ggacatcgcc gaggaccgcc
     6961 agttcaagaa cctcttcgag atctggctgg agggggacca ctacaagtgg cgcgccatgc
     7021 gctgcaacgg cgtgcccgag cacctcatca ccggcgatgc cacgccgaag gaaaaattcc
     7081 tcgcctgggc ggccacggtg ccccacaccc tgcgtaaccc gctttaccac tggacccatc
     7141 tggaactgaa acgctacttc ggcattgacg agctgctcag tgaaaagacc gccgagtcca
     7201 tctgggaacg gaccgaggcg gcgctggcca ggccggaaat gagcgcgcgc ggaatcctgc
     7261 gcgcgcaaaa ggtgcgcgct ctctgcacca cggacgaccc cgccgacgat ctcgtctggc
     7321 acaggaagtg cgcgcaggac ggcttcgagg tcggcgtcta tcccaccttc cgcccggaca
     7381 aggccttcgc cattgacaac ctggaggcat ggaatgcctg ggtggaccgg ctggagtccg
     7441 cctccggcaa gtccatccgc aaattccagg acctgctcga cgcgctcgcc gcccggcacc
     7501 aggccttcca cgacattggc tgccgcctca gcgaccacgg cctgaaccac tgccacgcca
     7561 gcttcgtgcc caggatcagg cttgaaaccc ttttcgagag gctccgcaac aaccgcagca
     7621 agcggattta ccccgaggac agggagatgc tgggcaccgc cgtcatgctc catgtcggcg
     7681 agatgaacgc cgacaaaggc tggacgatgc agctccacct cggccccctg cgcaacaaca
     7741 acagccgcct ggccaggctg gtcggcgcgg atgtcggctg cgacagcatc ggcgactatc
     7801 cgcaggcgca ggcgctgagc cgcttcctcg acgcgctcga ctcccaaggc aagctgccca
     7861 agaccgtgct ctacaacgtc aaccccgcgg acaactacgt cttcggcacc atggccggga
     7921 atttctcgga cggagtcacg ccgggcaagg tgcagttcgg ctccggctgg tggttcgtgg
     7981 atcaaaagga gggcatggag tggcagatca acgcgctgag caatctcggc ctgctctccc
     8041 gcttcatcgg catgctcacc gacagccgca gcttcatgtc cttcccccgg cacgagtact
     8101 tccgccgcac gctgtgcaat cttctcggcc gcgatgtgga ggagggcgtc atccctgacg
     8161 atgacgaact caccggcgcc atgatccgca acatctgcta cgccaacgcc aaggcctacc
     8221 tcggcctgga gaaaagctga gcgcgctcca gctcgcaggc gcgcttgcca aagcgcgggg
     8281 ttgactccca gtcgcaccca cagtctccgc attctggcga atgcgcctgc ggtgtgtatt
     8341 caaccaagca gcttctccag tgccgacgcc tcagtgaccg gggcctggca ggtgtagttc
     8401 cggcagacat aggcggccgg tttgccggag acgggtttca tttccgccag cgcggcattg
     8461 cgcgcgccca gccactcctg cgcctcgccg ccgccggcgt gcaggaccac ggcgtggggc
     8521 agcagccgct tgtggaccac gcccgccagc gcctgaaact ccgcgctgct gctgtcgccc
     8581 gccagcacga tctgctgctt gccgcgctcg atgaagtccg ccgccatcac cagcaccggc
     8641 accgaggagg gcgagccgtc cagcgtgccg ccaaaaagcg ccaggatttt ccgcgcctgc
     8701 tccagccacg gggcgtggtc gagcatggcg gcgaggcggg ccaggttcag cgcggcgagc
     8761 gagttcgggg agggctcggc gccgtcgtag tcctccttga tgcgcaggac ggagtgcgcc
     8821 atgtccgtgt ggacgctgta gtagccaccc ttttcggcgt cgagaaaaag cgcgtccatc
     8881 tcctcctgga gcgccgccgc ccagcgcagc cagcggctgt cgaaaccgga ctggtaaagg
     8941 tccagcagcc cgtggatgag gcaggcgtag tcggcggcga aagcgcgcgg tccgcgcccg
     9001 ccctcgcgcc agctccggtg caggcccttg ccgggttcat cgcagagctg gtcatgcagg
     9061 aaggaggcgg cctgcgcggc catgtcgagg aaggacttct cccccagcgc ggctcccgcg
     9121 cgggccaggc cggcgatcat gaggccgttc caggcggtga tgatcttgtc atccagatgc
     9181 gggcgggggc gcttggcgcg cgcgtcgaag agcaggcgca agccgtcggc caggatagtc
     9241 tgcacctcct ccacgggctt tttgaaatac tccgccgtct tcttcgcgga gaaggccttg
     9301 aacagcgtgt tccacccggc cagctcgccg tgggggtcgc tctccggccg cgcattgcca
     9361 tcccgccgcg cgccgtaggc gtaacgaaaa atgccgccgc gctccctgcc cagcagatcg
     9421 tctatctccg ccgccttcca gacataaaag gcgccctcgt gcttgtgcgg ctcgtccgcg
     9481 gagcgcaggc tgtcggcgtc ctcggcggag aaaaagccgc cgtccgggtg gcgcatgtcg
     9541 cgcgcgcagt aggcggcgat ctcgcgcgcc acaccggcca ggaagacgga gcccgtggcc
     9601 aggtgcccct cggcgtaggc ccagagaagc tggccctggt cgtagagcat cttttcataa
     9661 tgcgggatgt gccagtagcc gtccacgctg tagcggtgga agccgccgcc gacatggtcc
     9721 cagatgccgc cattggccat cgcccgcagg gtgcgcgcgc acatctcgcc ggcccagtcg
     9781 gactcgcttt tgcgctccgc gctcttcgcc caggccaggt gaatgcgcag cagcagattg
     9841 atggaggtgg agcgcgggaa cttcggcgcg ccgctgaagc cgccctcatg gtagtcgtag
     9901 ccgccggaca actcgtcgta ggccttctgc aggacggcgt ccgcagccac ctcgcggccc
     9961 tggttttcac cgtcgaggaa ctcctgcagc ttgctcaccg agcgctcggc gcccgccagc
    10021 accttctcgc ggtcctccgt ccacgccttg tgcagctcct tgagcaggtt cttgaagccg
    10081 atgcggccct gcgcgttctc cggcgggtaa taggtgccgc cgaaaaacgg cttcaaatcc
    10141 ggcgtgagga agaccgtcat cggccagccg ccgccgccgc tggccgcctg cacataggtc
    10201 atgtaggtga ggtccacgtc cgggcgctcc tcgcggtcca ccttcacggg cacgaagtgc
    10261 tcgttgatgt aggcggcgat ctcctcgttc tcaaacgact cgcgctccat gacatggcac
    10321 cagtggcagg tggagtagcc gctggagagg aagatgggct tgtcctccgc cctggctttg
    10381 gcgatcgcct catcccccca cggcagccag tccacgggat tgtgcgcgtg ctgcagcagg
    10441 tagggagact tctcccgggc cagggcgttg gtgtgtttcg gcggtgtctt ttggggatcg
    10501 ttcatgatga ggcaaacgtg gagagcgcgg gcggggttgc aagacacgcg gcttgggcgc
    10561 gcccggcgcg cgccgccctt cgaatttcga atttcgattt tcatttcccg ccttaccctt
    10621 gccgccgtgt cccagccctc ccagccctcc tggtggcagc tcctgcgcag tctgctgcgc
    10681 caggggcgcg ccatggtgcg gggcacgctc acggacgcgc cgctggaccc gctgccgctg
    10741 cgcaaaagac tccgcggcta caatctgccc aagctccgct ccgatgtgaa ggcgggcgcc
    10801 aatgtcgccc tgctcgacat tccccagagc atggcctacg ccgccgtggc cgggctgccg
    10861 ctccagttcg gcgccatgtg ctcggcggtg gccggcacgc tcggcgcgct cttcggcagc
    10921 tcccgcttca ccgtgctggg gccgacgaat gccaccgcgt tcatgatctt ctcctacttc
    10981 gccgcagacc cgcacctgga ccgcgccgcg ctcatgccgc tgctcgtctt catggtcggc
    11041 gcgctgctca tcatcggcgc ctggctgcgc gtggcggagc tggcgcagta catcagccgc
    11101 gccgtcatcg tcggctacgt caccggcgcg gcgctgctca tcatcgccaa ccagcttccc
    11161 aacgtgctcg gcatctccct gctggtggaa atgcccgacg gcggcgtctt ccagccgcgc
    11221 accatgcccg gcatcctctg gcagcttgcg cggaaaattt ttgaaacctc cctgcccggc
    11281 ctctgcctcg ccgcgggcac cgcgctggtc tattggggcg cgcgccgcct ccgccccgcc
//
LOCUS       DAOMYJ010000173        11338 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Prosthecobacter sp. isolate AMR_MDS_5629
            NODE_1903_length_11338_cov_20.712133, whole genome shotgun
            sequence.
ACCESSION   DAOMYJ010000173 DAOMYJ010000000
VERSION     DAOMYJ010000173.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867850
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prosthecobacter sp. (wastewater metagenome)
  ORGANISM  Prosthecobacter sp.
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Verrucomicrobiaceae; Prosthecobacter.
REFERENCE   1  (bases 1 to 11338)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11338)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.30
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:34:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,742
            CDSs (total)                      :: 4,684
            Genes (coding)                    :: 4,676
            CDSs (with protein)               :: 4,676
            Genes (RNA)                       :: 58
            tRNAs                             :: 55
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11338
                     /organism="Prosthecobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1903_length_11338_cov_20.712133"
                     /isolate="AMR_MDS_5629"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1965333"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..192
                     /locus_tag="PLP58_15990"
     CDS             <1..192
                     /locus_tag="PLP58_15990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ10081.1"
                     /translation="PRHAGCGSPGHFALRKPCTSRRPPRRTASVAAAGSIAALREGVL
                     DFDPNGMNRQPPFKGACRA"
     gene            319..756
                     /locus_tag="PLP58_15995"
     CDS             319..756
                     /locus_tag="PLP58_15995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ10082.1"
                     /translation="MASITADPTSVQPYDVRPLTWEEVKSAWDAAIGNLRVCVTFSAA
                     KAACRAARDKFIYLGTQLGSSSVSDEILDPAREARAEFEEAAALISAFPCDTLEAGQQ
                     IEFARFHENISLRARLIAQRLDEGREAFLRSLRKQAAARRLAA"
     gene            777..1748
                     /locus_tag="PLP58_16000"
     CDS             777..1748
                     /locus_tag="PLP58_16000"
                     /inference="COORDINATES: protein motif:HMM:NF021640.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide-binding protein"
                     /protein_id="HRJ10083.1"
                     /translation="MMIQALNKLVCRSILREALEMASTQADLHGLELGIEHSGEAGHH
                     HRLAMVLHNELHALFETKRIFAKLSKDTPDAKALMEHLRDAVADVNRIVKQLDQAVAD
                     PSQWPRIHADLVKGGAYQQISERIEPLLAGLADFHQSALREFQAERAPCRLPARPSVF
                     LASTTAGIPLAEKIQAALQSELPQLHLDLWTKALSLGGSILGALEGALSRYQMAVCII
                     GPQDSFSPRRRKPDWLPRWLDKSVGKDPADDRMPIGNVILELGMFIGQHTSARAFLVQ
                     PDQGIPSLSDLDGLLTARYQRGLSDDEITSAVLPKLAQAMKAEMARG"
     gene            1901..2806
                     /locus_tag="PLP58_16005"
     CDS             1901..2806
                     /locus_tag="PLP58_16005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009963780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HRJ10084.1"
                     /translation="MAKSALGKGLAALISAPSAVKGVASPPPLAQPAPGDVVNRVPMD
                     QVVPSPLQPRKEFVQEQLRELMDSIREHGIIQPLIVRPVNGKLELIAGERRYRASREL
                     GLAEVPVITRQASDKDVLEMALIENLQREDLNPIEEARAYERLSKDFGMRQEDIAQRV
                     GKNRATVANTMRLLDLCEPVQKMLSGRQLSTGHAKVLLTLKDAGEQEKAAAEIVRRSL
                     TVRGAEKLAQAVLHPPQPKPQLGDNEEARAIAAVKQRLMDRLITGVAITHGEKKGHIQ
                     IDYYGVEDLNRILALMGVPEEQLED"
     gene            2814..3257
                     /locus_tag="PLP58_16010"
     CDS             2814..3257
                     /locus_tag="PLP58_16010"
                     /inference="COORDINATES: protein motif:HMM:NF024297.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HRJ10085.1"
                     /translation="MRPPTAVFLSICWLLAGCGGAAEHPRRAEISAFLDRYFSTWSAR
                     DMEGYGACFDPAARVVFVEKNGRSSSQGLTDFLHGQKLGHERSPVPMKETPAAMKISG
                     DTRVACAEVPWRLEKGREVVTGVDYFTLARDSKGWRILALVFYND"
     gene            3285..4826
                     /locus_tag="PLP58_16015"
     CDS             3285..4826
                     /locus_tag="PLP58_16015"
                     /inference="COORDINATES: protein motif:HMM:NF037959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused MFS/spermidine synthase"
                     /protein_id="HRJ10086.1"
                     /translation="MPKSPEPTAGLARLERLYLGTACFCAGAAMMVIEISAARLLAPM
                     FGNSIYTWTALIGVILISFSIGGYLGGRLADRRAGIEVLGWLLAVAAVLTFAIPALGA
                     LITPVLEGSGFIDGPVFYSLLVLAVPGALLGAVSPASVRLYSLTWKDTHVGGAAGTVS
                     MLGSLGSFVGTFLSGFVLLGAFGVKSIFIGCALLLLLLALCAFALGRVLMKSGVRVVV
                     AAVLAGGTSAVSKTPANESVIHERESYYHHIKVIETGAAPRRERRLQLDSTSEGGINP
                     DTGALILDYQHFWRLAAMDGRKISSALFIGAGAFGMPCELSREHPGASVDVAELDPQV
                     IEVGREFFNLDKHPKVRAHAGDARHFLRTRADNRWDMIFGDAYNGVHAIPPHLATREF
                     FQLVSDRLSPDGVYLMNIISAVQGRHAELLGGMLSTLREVFPNVEVFAVHPGVRQTVQ
                     NIIIMASHQNRLESVTGRRHTSGSVAARLAASHIPARQAPASGVVFTDDFNPVDSIIA
                     RSLLR"
     gene            complement(4830..5138)
                     /locus_tag="PLP58_16020"
     CDS             complement(4830..5138)
                     /locus_tag="PLP58_16020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ10087.1"
                     /translation="MIHGLLDATQVVATVELDGAPHEVCCEAEASHDRRTNLLTVRLH
                     AFVRAMEQDHIGETATPAWLPEPETVTESVDLDEAHEMAEDIFASWNRRVLAALPRNP
                     "
     gene            complement(5170..5751)
                     /locus_tag="PLP58_16025"
     CDS             complement(5170..5751)
                     /locus_tag="PLP58_16025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ10088.1"
                     /translation="MPATLDAGKTASSQAHSHEDILAPLVFFYGIGSARPRVTFIQPE
                     ELPEHERWLLDHDMDMTPRLREGHGSEIDLDVHARARIGDYLVRASVLNRRSDGKPVE
                     FGAIGIHLDLLPEAARAQVIEGRVPFGAILENHAVPHSSHPRGFFRIAVDARLAELLG
                     ATSGQILFGRCNELRHGGGRVLADVVEVLPRGI"
     gene            complement(5735..7081)
                     /locus_tag="PLP58_16030"
     CDS             complement(5735..7081)
                     /locus_tag="PLP58_16030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009962340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="coproporphyrinogen-III oxidase family protein"
                     /protein_id="HRJ10089.1"
                     /translation="MSSTLESTAPAAEKKVDKTEAGNYFVSNYPPFSFWKPEQIPAVK
                     EVLARAAPADVPLGVYFHIPFCRKRCHFCYFKVYTDKNAQEIKGYIDAGMREMAHYAA
                     QPYIAGRSAHFVYFGGGTPSYLSVPQLKDLTRRMKDLVSWEGAAEVAFEAEPGTLNEK
                     KLEGIREVGVTRLSLGVENFDDHILETNGRAHRSGEIEKSYRFARTLGFEHINIDLIS
                     GMMNETEENWRTCVEKAVQLGPDAVTIYQMEVPYNTTMYQQMKAEGKVTAPVADWPTK
                     RRWVSYAFDEFVKAGYTVTSAYTVVKDPRRIKFIYRDSLWEGSDLLSCGVASFGHLGG
                     VHYQNQADIGPYMQLLDAGVPPIFRAYQTNSEERFVREFILKMKLGRSEFAYYQEKFG
                     VNPRRFFAQQLEWLAHEGFASLDDDSITLTRDGLLQVDRLLHDFFLPQHRQYARYT"
     gene            complement(7210..7767)
                     /gene="sufT"
                     /locus_tag="PLP58_16035"
     CDS             complement(7210..7767)
                     /gene="sufT"
                     /locus_tag="PLP58_16035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009960485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative Fe-S cluster assembly protein SufT"
                     /protein_id="HRJ10090.1"
                     /translation="MHSSLELKREVEAIQIPSGDSIRLPLGMKVIITQSLGGTYTVAT
                     DFGLARISAKDAEALGIDPDASARDSESSSSNVPEDATELEGAVWNQLKNVYDPEIPV
                     NIVDLGLVYDCRIEQDAEGKNRAVVKMTLTAPGCGMGPTIAADAQSRIMTIEEMDDAV
                     VELVWDPPWNKEMISIEGKMKLGMI"
     gene            complement(8027..9577)
                     /locus_tag="PLP58_16040"
     CDS             complement(8027..9577)
                     /locus_tag="PLP58_16040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ10091.1"
                     /translation="MSSSSFGIRHYFVILAYSFVITSLATAATPAEVTAAAIKAVAFY
                     HSHAAAHGGYVYRYSADFTFREAEGIPGPDTIWIQPPGTPAVGMAMLDAYEATKDEKC
                     LAAAVEAARCVSRTQLASGGWDYSGHFDAKGRESKNYCRKADGTAIPRTQTATREAGW
                     HNWRRQDKKNYATYDDDVSQAATRLLVRVDHALGGKDAEIKDAADFALRTIMMTQYPA
                     GGWSANFDDAPTFPPPVEKYPIKPANYPQDWPRKWPKDFSGCYVLNDNTHATLMSTLL
                     LAWQLRGDAQYLEAAKRGGDFLLLAQMPDPQPAWAQQYNADMQPVWSRQFEPTAISGR
                     ESQAAMWALLKLAAATGDKKYLAPVAKAITYLRTVLLPGNKLARFYELNTNKPLYFER
                     GPGGKGFELTYSDKRASSNYGWVWDSELDALQAAGSRIARGEPVTFPRTEKERWSSPP
                     TDADIATILKEQAPNGSWPVTDDERGWMRDESGKKTKPAGGVIYSLDFVQNVKAMSAW
                     LKAKGGVK"
     gene            complement(9709..>11338)
                     /locus_tag="PLP58_16045"
     CDS             complement(9709..>11338)
                     /locus_tag="PLP58_16045"
                     /inference="COORDINATES: protein motif:HMM:NF017390.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="M56 family metallopeptidase"
                     /protein_id="HRJ10092.1"
                     /translation="PTRAMTADSLLHWLQRTSLEASILIFAVLGLRLMLGTRLSPAWR
                     IGLWMLVGAKLMLPAFIPAGFGLGAWAKEETAAVTEVIQAASPAKQTSVLALNTISSP
                     DVPAERSWSFSLRILLTWIWLLGAAAVLFGALFRQRRFDRYLRTRRIANDPHLCSLLR
                     SLSSRAGVSQTPQAVLMPAGTTPAMVGIRQPKLLLPEDWSTRFDKKSLRHIVLHELLH
                     VKQHDLLWNWAATAVQALHWFNPLVWFVVSRFQADRELRCDAGALAILSPAERLDYGH
                     TLLRIQETFFAPPAIAGLAPCVRNHPTLRQRILMIATPTTRQPVIQALLVLTLSVLVG
                     YSFTTARAAEKEVPPKVREGATTAKPSTESPRSTESPKPAMKDGEREGGKPSAERDGG
                     KPSAERDGTRKPGPRDGEGEKKPGMRDGDKPRTGSRDGEGMKKGGERDGEGRKPSAES
                     GQKPRKSEGDREATSSGEVITMHVPASGDTILINGEERPLSGLRGFLKDFLPEHPGAK
                     VLVSGDPDTPLKSLHQIIDAVRDNGNKNVGIKAE"
BASE COUNT         1907 a         3455 c         3818 g         2158 t
ORIGIN      
        1 ccccgccacg ccgggtgcgg cagtcccggc cacttcgccc tccgcaaacc ctgcaccagc
       61 cgccgccccc cccggcgcac tgccagtgtc gccgccgccg gcagcatcgc agccttgagg
      121 gaaggcgtgt tggattttga tcccaatggg atgaaccgcc agccgccatt caaaggggca
      181 tgcagggctt gaaattcccg ctggcataac gacaaagatg tctgatgatg ccggactcgg
      241 ccggactcta aggggaggcg tttatttaaa cttccctttg acctcctggc cggttctctg
      301 ctacagcctt cccaaatcat ggcctcaatc accgctgatc ccacgtccgt ccagccctat
      361 gatgtgcgtc ccttgacctg ggaggaagtg aaatccgcat gggatgcagc catcggaaat
      421 ttacgcgttt gcgtcacctt ttccgctgcc aaggccgcat gccgcgccgc tcgtgacaag
      481 ttcatctacc tcggcaccca gctcggcagt tcatccgtca gcgatgaaat cctggaccct
      541 gcccgcgagg ctcgcgccga attcgaggag gcggcggcgc tcatcagtgc ttttccatgt
      601 gacacgcttg aagccgggca gcaaatcgag ttcgcccgtt ttcacgaaaa catttccctg
      661 cgcgcgagac tcatcgcgca gaggctcgac gaggggcggg aggcgttcct gcgctccctg
      721 cgcaagcagg cggcagcccg aaggctggcg gcctgaccat ccccctcctg ccggccatga
      781 tgatccaggc actgaacaag ctcgtatgcc gctccatcct gcgcgaggcg cttgagatgg
      841 ccagcaccca ggccgacctg cacggtttgg aactgggcat tgagcactcc ggagaggccg
      901 gccatcatca ccgtctcgcg atggttctgc acaatgagtt gcacgccctg ttcgagacga
      961 agcgcatctt tgccaagctc tccaaagaca ccccggatgc caaggctctc atggagcatc
     1021 tgcgtgatgc cgtggcagac gtaaaccgga tcgtcaaaca actcgaccaa gccgtggcag
     1081 acccctcgca gtggccgcgc atccacgccg atttggtcaa aggaggcgcc tatcagcaga
     1141 tcagcgagcg cattgagccg ctgctggcag ggctggcgga ctttcaccag tcggcattgc
     1201 gcgaattcca ggccgagcgc gcgccctgcc gcctgcccgc gcgaccatcc gtcttcctgg
     1261 cctccaccac tgcgggcatt cctctggcgg aaaaaatcca ggctgccttg cagtcggaac
     1321 tcccacagct tcatctcgat ctttggacga aggctctcag ccttggaggc tcgattctgg
     1381 gcgctttgga gggcgctctt tcccgctatc agatggccgt ttgcatcatc ggtcctcagg
     1441 acagcttctc cccgcgcagg cgcaagcctg actggctgcc gcgctggctg gacaaaagtg
     1501 tcggcaaaga ccctgccgac gaccgcatgc ccattggcaa cgtcatcctg gagttgggca
     1561 tgttcatcgg gcagcacact tcggcgcgcg cgttcctcgt gcagcccgat cagggcatac
     1621 ccagcctcag tgatctcgac ggcctgctca ccgcgcgtta ccagcgcggt ttgagcgatg
     1681 atgagatcac ctccgcagtc ctgccgaaac tggcgcaagc gatgaaggcg gaaatggcgc
     1741 gcggctgagg caaggccgcg cgcatccaaa atcccggcgc tgtgaaatgg ggcgtccgcg
     1801 gcgctccgcg cgcaaaaaaa caacgcaaat tcaaggctgc ggaggcggcg tggcaaaagc
     1861 agtttgccat ccggggcgcg ctcattaacc tcgccgccct atggctaaat ctgcacttgg
     1921 aaaaggactc gccgcgctga tctcggcgcc ctcggcggtc aaaggggtgg cgagtccgcc
     1981 cccgctggcg caacccgcgc cgggggatgt ggtgaaccgt gttccgatgg accaggtggt
     2041 gcccagcccg ctgcagccgc gcaaggagtt tgtgcaggag cagctccgcg agctgatgga
     2101 ttccatccgc gagcacggga tcatccagcc gctgatcgtc cggcctgtga acgggaagct
     2161 ggagctgatc gctggcgagc gccgctaccg cgcctcgcgg gagctggggc tggccgaagt
     2221 gccggtcatc acccgccagg ccagcgacaa ggatgtgctg gagatggcgc tcatcgagaa
     2281 tctgcagcgc gaggatctga accccatcga agaggcgcgc gcctatgagc ggctgtcgaa
     2341 ggatttcggc atgaggcagg aggacatcgc ccagcgcgtg ggcaaaaacc gcgccaccgt
     2401 ggccaacacc atgcgcctgc tggacctgtg cgagccggtg cagaaaatgc tctccggcag
     2461 gcagttgagc accgggcacg ccaaggtgct gctgacgctc aaggacgccg gcgagcagga
     2521 gaaggccgcc gccgaaatcg tgcgccgcag cctcacggtg cgcggcgcgg aaaagctggc
     2581 gcaggctgtg ctgcacccgc cgcagcccaa gccgcagctc ggcgacaacg aggaggcgcg
     2641 cgccattgcc gccgtgaagc agcggctgat ggaccgcctc atcaccgggg tggccattac
     2701 tcacggggag aagaaggggc acatccagat tgactattac ggtgtcgagg atctgaaccg
     2761 catcctggcg ctgatgggcg tgccggagga gcagctcgaa gactgatccg cccatgcgcc
     2821 cgccgaccgc cgtctttctt tccatctgct ggctgctcgc cgggtgcggc ggcgcggccg
     2881 agcatccgcg gagggctgag atcagcgcgt tcctggatcg ctacttctcc acctggtccg
     2941 cgcgggacat ggagggctac ggcgcgtgct ttgatccggc ggcgcgcgtg gtcttcgtcg
     3001 agaaaaacgg gcgctcctcc agccaggggc tgactgattt cctgcacgga cagaagctgg
     3061 ggcacgagcg ctccccggtg cctatgaagg agacgcccgc ggccatgaaa atcagcggcg
     3121 acacgcgcgt ggcctgcgcc gaggtgccgt ggcggctgga gaagggcagg gaggtggtca
     3181 cgggagtgga ctacttcacg ctcgcgcgcg actccaaagg ctggcgcatc ctggcgctgg
     3241 tgttttacaa cgactgagtc tcttctgttt tcccctgatt ccccatgccc aaatcccccg
     3301 aacccaccgc cggcctcgcg cgcctggagc gtctttatct cggcacggcc tgcttttgcg
     3361 cgggcgcggc catgatggtc atcgagatca gcgccgcgcg cctgctggcg ccgatgtttg
     3421 gcaacagcat ctacacctgg acggcgctca tcggggtgat cctcatctcc ttcagcatcg
     3481 gcggctacct cggcggcagg ctggcggaca ggcgagcggg catagaggtg ctcggctggc
     3541 tgctggcggt ggcggctgtg ctcacttttg ccattccggc gctgggcgcg ctgatcacgc
     3601 ccgtgcttga gggcagcggt ttcattgacg gcccggtctt ttactcgctg ctggtgctgg
     3661 ctgtgccggg cgcgctgctc ggcgccgtct cgccggcctc ggtgcgcctt tacagcctga
     3721 cgtggaagga cacgcatgtc ggcggcgcgg cgggcacggt gagcatgctc ggctcgctgg
     3781 gcagcttcgt cggcacgttt ctctcgggct tcgtgctgct gggggctttc ggcgtgaagt
     3841 ccatcttcat cggctgcgcc ctgcttctgc ttcttcttgc gctgtgcgcg ttcgccctcg
     3901 ggcgggtgct gatgaaaagc ggcgtcagag tggtcgtggc cgcagtgctg gcgggcggga
     3961 ccagcgccgt gtcaaaaacg cccgcgaacg agtctgtcat ccacgagcgc gagtcctact
     4021 atcatcacat caaggtcatc gaaaccggcg ccgcgccgcg ccgcgagcgc cgtctgcagc
     4081 tcgactccac ctccgagggc ggcatcaatc cggacaccgg agcgctgatc ctcgactacc
     4141 agcacttctg gcgtctggcc gcgatggacg ggagaaaaat ctccagcgct ttgttcattg
     4201 gcgcgggagc tttcggcatg ccgtgcgagc tttcgcgcga gcatcccggc gcttcggtgg
     4261 acgtggcgga actggacccc caggtcattg aggtggggcg cgagtttttc aacctggaca
     4321 agcatccgaa ggtgagggcg cacgcgggcg acgcgcgcca cttcctgcgc acccgcgcgg
     4381 acaaccgctg ggacatgatt ttcggcgacg cttacaacgg cgtccacgcc atcccgccgc
     4441 atctggccac gcgcgagttc tttcagctcg tgtccgaccg tctttccccc gatggcgtct
     4501 atctgatgaa catcatctcc gccgtgcagg ggcggcacgc cgagctgctg ggcggcatgc
     4561 tttccacgtt gcgggaggtt tttccgaatg tggaggtctt cgccgtgcat cccggcgtgc
     4621 ggcagacggt gcagaacatc atcatcatgg cctcacatca aaaccggctg gaaagcgtca
     4681 ccggccgccg ccacaccagc ggcagcgtgg ccgcgcgcct cgccgccagc cacatacccg
     4741 cgcgccaggc gccggccagc ggcgtggttt tcacggatga tttcaatccg gtggactcga
     4801 tcatcgcccg cagtctgctg cgctgaggat cacggatttc tgggcagggc cgccagcacg
     4861 cgccggttcc agctcgcgaa gatgtcctcg gccatttcat gggcctcgtc caggtccacg
     4921 gactccgtca ccgtttccgg ctccggcagc cacgcgggcg tggccgtctc gccgatgtga
     4981 tcctgctcca tcgcccgaac gaacgcatgg agcctgacgg tgagaaggtt ggtccggcgg
     5041 tcatggctcg cctccgcctc gcagcagact tcgtgagggg cgccgtccag ttcgacagtg
     5101 gcaacgacct gggtggcatc aagcaaaccg tggatcatgg ccgaatccaa gattgaacac
     5161 gccgctttgt caaatcccgc gtggcagcac ctcgaccacg tcggccagaa cgcgtccgcc
     5221 gccgtggcgc agctcgttgc agcggccgaa caagatctgg ccgctggtgg cgccgagaag
     5281 ctcggcaagg cgcgcgtcca cggcgatgcg gaagaagccg cgcggatggc tgctgtgcgg
     5341 aacggcgtgg ttttccaaaa tggcgccgaa gggcacgcgc ccctcgatga cctgcgcgcg
     5401 cgcggcctcc ggcagcaggt ccaggtggat gccgatggcg ccgaactcga ccggtttgcc
     5461 gtcgctgcgg cggttgagca cggaggcgcg cacgaggtag tcgccgatgc gcgccctggc
     5521 atgcacgtcg aggtcaattt cgctgccgtg gccttcgcgc aggcgcggcg tcatgtccat
     5581 gtcatgatcc agcagccagc gctcgtgctc cggcagctcc tccggctgga tgaaggtgac
     5641 gcgcggcctg gcgctgccga tgccgtaaaa gaagaccagc ggggcgagaa tgtcctcgtg
     5701 ggagtgcgcc tgtgaggagg ctgtttttcc ggcgtcaagt gtagcgggca tattgacggt
     5761 gctggggaag gaagaaatca tgcagcaggc ggtccacttg caggaggccg tcgcgcgtga
     5821 gggtgatgga atcgtcgtcc agggaggcga agccctcgtg cgcgagccac tcaagctgct
     5881 gtgcgaagaa ccggcgcgga ttgacgccaa acttctcctg gtaataggcg aactcgctgc
     5941 ggccaagctt catcttcagg atgaactcgc gcacgaagcg ctcctccgag ttggtttgat
     6001 acgcgcggaa aatcggcggg acgccggcgt ccagaagctg catgtagggg ccgatgtccg
     6061 cctggttctg gtaatgcacg ccgcccagat ggccgaagga ggccacgccg caggagagca
     6121 ggtccgagcc ctcccagagc gagtcgcggt agatgaactt gatgcgcctc gggtccttca
     6181 ccaccgtgta ggcgctggtg acggtgtagc ccgctttgac aaactcgtcg aaggcgtaac
     6241 tgacccagcg gcgcttcgtg ggccagtcgg ccacgggggc ggtaaccttg ccctcggcct
     6301 tcatctgctg gtacatggtc gtgttgtagg gcacctccat ttggtaaatc gtcacggcgt
     6361 ccggccctag ctggacggcc ttctccacgc aggtccgcca gttttcctcg gtctcgttca
     6421 tcatgcccga gatgaggtca atgttgatgt gctcgaagcc cagcgtgcgg gcgaagcggt
     6481 aggatttttc aatctcgccg ctgcggtgcg cgcggccgtt ggtctccaga atgtggtcgt
     6541 caaaattttc gactcccagg ctcaggcggg tgacgcccac ctcgcggatg ccctccagct
     6601 ttttctcgtt cagcgtgccc ggctccgcct cgaaggccac ctccgccgcg ccctcccagg
     6661 agacgaggtc cttcattctc cgagtcaggt ccttgagctg cggcacgctc aggtagctcg
     6721 gcgtgccgcc gccaaaatag acgaagtgcg cactgcgccc tgcgatataa ggctgcgcgg
     6781 cgtaatgcgc catctcgcgc attccggcgt caatgtagcc cttgatctcc tgcgcgtttt
     6841 tgtcggtgta aaccttgaag tagcagaaat ggcagcgctt ccggcagaag ggaatgtgga
     6901 agtacacgcc aagcgggaca tccgccggcg cggcgcgcgc cagcacctcc ttcaccgccg
     6961 ggatttgctc aggcttccaa aacgagaagg gcgggtagtt cgagacgaaa taattgcccg
     7021 cctcggtctt gtcgaccttt ttctcggcgg cgggcgcggt ggattccagg gtggaggaca
     7081 tgcgggggtg gatatgtgaa cgcggaggcg cgctcatttc taaaccacaa aaccgagttt
     7141 ggcagcacgc aatgctcctc tcgatgacgc gtttgagaaa gcgcgggcgt caacgccggg
     7201 aggatgccct cagatcatcc ccagcttcat tttgccctcg atggagatca tttccttgtt
     7261 ccacggcgga tcccagacca gttcgacgac ggcatcgtcc atctcctcaa tggtcatgat
     7321 gcggctttgc gcgtcggcgg cgatggtggg acccatgccg cagccggggg cggtgagcgt
     7381 cattttcacc acggcgcggt ttttgccttc cgcgtcctgc tcgatgcggc agtcatacac
     7441 gaggccgagg tcaacgatgt tcacggggat ctcggggtca tagacatttt tgagctggtt
     7501 ccataccgcg ccttcgagtt cggtggcgtc ttcgggcacg ttggacgagc tgctttccga
     7561 gtcgcgggcg gaggcgtccg ggtcaatgcc cagcgcttcg gcgtccttgg cggagatgcg
     7621 cgccaggccg aagtcggtgg ccaccgtgta ggtgccgccg agggactggg tgatgatgac
     7681 cttcatgccc aggggcagcc ggatggagtc gccgctggga atctggatgg cttcgacttc
     7741 gcgtttgagt tcgagtgagg agtgcatggt ctgtggcggg gcggcggtct gaaactccgc
     7801 atggcgtaat tacgaacgaa aatcaacccg gagagcgtgc taaatgccgc tcgccagttc
     7861 ggcaaaggcc ggggaaaaag cggagcgggg tgagttcggt gggaaagtct tccgataacg
     7921 ctgcatacgc ggcgcccaga catccggctt ccgggacatg tgccggcggc cggattcggc
     7981 gctatgaatg attgtcttca cccccgcctt acggccggac agtgcttcat ttcacgcctc
     8041 ccttggcttt cagccacgcg ctcatggctt tgacgttttg gacgaagtcg aggctgtaga
     8101 tcacgccgcc ggcgggcttc gttttcttgc cgctttcatc tcgcatccag ccgcgttcgt
     8161 catcggtgac gggccaggag ccgttggggg cttgttcttt gaggatggtg gcgatgtcgg
     8221 cgtcggtggg cggggaggac cagcgttctt tttcggtgcg ggggaaggtg acgggctcgc
     8281 cacgggcgat ccggctgccg gcagcttgca gggcgtcgag ttcgctgtcc cagacccagc
     8341 cgtagttcga ggaggcgcgt ttgtcggagt aggtgagctc gaaacctttg ccgccggggc
     8401 cgcgctcgaa gtagaggggc ttgttcgtgt tcagttcgta gaagcgggcg agcttgttgc
     8461 caggcaggag cacggtgcgg aggtaggtga tggccttggc gacgggcgcg aggtattttt
     8521 tgtcgccggt ggcggcggcg agcttcagca gcgcccacat ggcggcctgg ctttcgcgac
     8581 cgctgatggc ggtgggctcg aactgacggc tccacacggg ctgcatgtcg gcgttgtatt
     8641 gctgcgccca ggcgggctgc ggatcgggca tctgcgcgag caggaggaag tcgccgccgc
     8701 gtttggcggc ctcgaggtat tgggcatcgc cacggagctg ccaggcgagg agcagcgtgc
     8761 tcatcaaggt ggcgtgcgtg ttgtcattga gcacgtagca gccgctgaag tccttcggcc
     8821 acttgcgcgg ccagtcctgc gggtagttcg cgggcttgat gggatacttt tcgacgggcg
     8881 gcggaaaggt gggcgcgtcg tcgaaattag cgctccaacc gccggcggga tactgcgtca
     8941 tcatgatcgt gcgcagcgcg aagtcggcgg cgtctttgat ctcggcgtcc ttgcccccga
     9001 gcgcgtgatc gacacgcaca agcaatcgcg tcgcggcctg cgagacatcg tcgtcgtagg
     9061 tggcgtagtt tttcttgtcc tgacgacgcc aattgtgcca gccagcctca cgcgtggcgg
     9121 tctgcgtgcg aggaatggcc gtgccatcgg ccttgcggca ataattcttc gattcgcggc
     9181 ccttggcatc gaaatggccc gagtagtccc aaccgcccga agcgagctgc gtgcgcgaaa
     9241 cgcatcgcgc ggcctccacg gcggcggcga ggcacttttc gtccttcgtg gcctcgtaag
     9301 catccagcat cgccatcccg accgcaggcg tgcccggcgg ctgaatccag atcgtgtccg
     9361 ggccgggaat gccttcggct tcgcgaaagg tgaaatcggc gctgtagcgg taaacgtagc
     9421 cgccatgggc cgccgcgtgg gaatggtaga aggcgacggc tttgatcgcc gcggcggtga
     9481 cttcggcagg tgtcgcggcc gtcgcgagcg acgtaatgac gaatgagtaa gcaagaatga
     9541 cgaaataatg tcgaatgccg aatgacgaag aggacattgt tggcgaggct gttggtgctt
     9601 ttggcattgg gaattctttc gtcattcgga tttactcatt tgtcatttgc acgggcacga
     9661 agctcctgga gcaggccgtg ccccgagtgt tctcgatgat gacggtcgtt actcggcctt
     9721 gattccgacg ttcttgtttc cgttgtcgcg gacggcgtcg atgatttgat ggagagactt
     9781 gagcggggtg tcggggtcgc cggagacgag gactttggca ccagggtgct cggggaggaa
     9841 atctttgagg aagcctcgaa ggccgctgag cgggcgctct tcgccgttga tgagaatggt
     9901 gtcgccgctg gcgggaacgt gcatggtgat gacttcgccg gaggaggtgg cttcgcggtc
     9961 gccttcgctt ttgcggggct tttggccgga ttcagcggag ggtttgcggc cttcgccatc
    10021 gcgttcaccg ccttttttca tgccttcgcc gtcgcgggag ccggtgcggg gcttgtcgcc
    10081 gtcgcgcatg ccgggtttct tttcgccttc gccatcgcgg gggccgggtt tgcgggtgcc
    10141 atcgcgttcg gcgctgggtt tgccgccgtc acgctctgcg gagggttttc cgccttcgcg
    10201 ttcgccgtct ttcatggcgg gttttgggga ctctgttgac cgtggtgact ctgttgacgg
    10261 ttttgcggtg gtggcgcctt cgcggacctt gggcgggact tctttttcgg cggcgcgggc
    10321 ggtggtgaag gagtagccga ccaacacgct gagcgtgagg acgagcaggg cctgaatgac
    10381 gggctggcgg gtggttgggg tggcgatcat gaggatgcgt tgacgcaggg tggggtggtt
    10441 gcgcacacat ggggccaacc ctgcgatggc cggcggtgcg aaaaaggtct cctgaatgcg
    10501 cagcagcgtg tgcccgtagt cgagccgctc ggcgggagag agaatggcga gcgctccggc
    10561 atcgcaacgc agttcgcggt cggcctggaa gcgtgacacg acgaaccaca cgagcggatt
    10621 gaaccaatgc agcgcctgca cggccgtggc ggcccagttc cagaggaggt cgtgctgttt
    10681 cacatgcagc agctcatgca gcacgatgtg tctgaggctt ttcttatcga agcgtgtgct
    10741 ccaatcctcc ggcagcagca gcttgggctg gcggatgccg accatggccg gcgtggtgcc
    10801 tgcgggcatg aggacggcct ggggagtctg cgacacgcca gcacgagaac tgagcgaacg
    10861 cagcagggag caaagatgcg ggtcgttcgc gatgcgtcgc gtgcgcaggt agcgatcaaa
    10921 gcggcgctgc cgaaacaagg ccccgaaaag cacggctgcc gcgccgagca gccagatcca
    10981 ggtgagcaga attcgaagag aaaacgacca actgcgctct gcgggcacat ccggggagga
    11041 aatggtgttc agtgcgagga cggaggtttg tttcgctggc gatgcggcct ggatgacctc
    11101 ggtcacggcc gcagtctcct cttttgccca agctccaaga ccgaatccgg ccgggatgaa
    11161 ggcggggagc atcagcttcg ctccgacgag catccacagg ccgatgcgcc acgcgggaga
    11221 gagtcgcgtg ccgagcatga ggcgcaggcc gagcacggcg aaaataagga tgctggcctc
    11281 aagggaggtg cgctggagcc agtggaggag ggagtcggcg gtcatggcgc gggttggg
//
LOCUS       DAOMYJ010000174        11309 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Prosthecobacter sp. isolate AMR_MDS_5629
            NODE_1907_length_11309_cov_24.421006, whole genome shotgun
            sequence.
ACCESSION   DAOMYJ010000174 DAOMYJ010000000
VERSION     DAOMYJ010000174.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867850
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prosthecobacter sp. (wastewater metagenome)
  ORGANISM  Prosthecobacter sp.
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Verrucomicrobiaceae; Prosthecobacter.
REFERENCE   1  (bases 1 to 11309)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11309)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.30
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:34:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,742
            CDSs (total)                      :: 4,684
            Genes (coding)                    :: 4,676
            CDSs (with protein)               :: 4,676
            Genes (RNA)                       :: 58
            tRNAs                             :: 55
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11309
                     /organism="Prosthecobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1907_length_11309_cov_24.421006"
                     /isolate="AMR_MDS_5629"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1965333"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..645
                     /locus_tag="PLP58_16050"
     CDS             <1..645
                     /locus_tag="PLP58_16050"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HRJ10093.1"
                     /translation="VLCGEPPAGFTLPGSTRLPSPPRVPSGLPSTVMQRRPDIRAAEQ
                     NLRAANASIGAAKAEFFPAFRLVGTGGLESVGSEGFIQGRAKTASIGPQITLPVFQGG
                     RLFGGLRGAEARYHESLAAYKQTILTALREVEDSMLDVRTLGRQRAAVAAAVAAAQET
                     AKLSRLRYDRGLSNYFEVVDAERVVLTARLTLAQLDGQRLLAAVQMLRALGGGW"
     gene            922..1083
                     /locus_tag="PLP58_16055"
     CDS             922..1083
                     /locus_tag="PLP58_16055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ10094.1"
                     /translation="MLPAADTLDKILRYEIKLERQMYRAMDQLERIQRMRRGELIPAP
                     LSVAVSGRG"
     gene            1207..1980
                     /locus_tag="PLP58_16060"
     CDS             1207..1980
                     /locus_tag="PLP58_16060"
                     /inference="COORDINATES: protein motif:HMM:NF014035.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HRJ10095.1"
                     /translation="METLVIALCGFVAAGLTLLSGFGLGTLLMPVVTLFFPVEIAVSV
                     TAVVHLANNLFKLALVGWRADRGVVLRFGIPAVLAAFAGALTLGWLAHLPPLAEYSLM
                     GHTLAVMPVKLTVGLLILGFVAMELSPAMAAWRFDARLLPVGGCVSGFFGGLSGNQGA
                     FRSMFLLKTGLSKEQFIATGVVLAVIVDLARMPVYGVAFFKSGPPVDLTLVVVACLAA
                     FAGSFLAARLLKKLTIRSLQVIVGTLLILVAFGMITGVL"
     gene            2110..5247
                     /locus_tag="PLP58_16065"
     CDS             2110..5247
                     /locus_tag="PLP58_16065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007418437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HRJ10096.1"
                     /translation="MNLARFALRYPWTIIVAVVAVALGAWLGLQRMTRDIFPPLGIPT
                     IYVAQPYGGMDPLQMEGYLTYRYEYHFLYIANIEHVESKSIQGASIMKLQFHPGTDMS
                     QAMSETVAQVNRSRAFMPPGTVAPFIMRFDAGSVAVGHLVFSTDDPNITLNQMQDQAL
                     NKVRPAFATLPGVSAPPPFGGSSRGIVVNVNPDRMKAYGLSPDDIVQAIAKGNPISPS
                     GNMNLDGKYPIVPTNAIVSNVKDLEAVPLRHTDKGAVFIRDVATVADSADVTTSYALA
                     NGKRTVYLPVTKRAEASTLEVVKKVRESIPEFQKLLPDGIKVSFEFDQTPVVNQSIRD
                     LVKEGALGAVLTGLMVLVFLRDLRTAFIVVINIPLSLMAAAFGLWISGQNIHLMTLGG
                     LALAVGILVDEATVTVENIHTHLARGKTLARAALDGTLETTLPRLLAMLCILAVFIPA
                     FFMVGAAQALFVPLALAVGFAMFASFILSSTLVPVLSVWFLPKNTKHEEERLGLLARA
                     YQGVVKAAVTLRWLLVPAYLGGAVLIIVTFGPFLGSEIFPQSDSGQFAIRFRAPSGTQ
                     VGITEGIAQKILKIIGDEAPVEFSIGMVGVHNSSFPVNLVHLWNGGPEEGWLAVQLKK
                     DGSVEVPELQEKLRGIMKRELPEVRVSFEPQDIVSRVMSFGTSTPIEIAVSGPSLPAS
                     KEHAEKLLAKLKELPFLRDAQIAQTLDAPTVNVQIDRERAGLLGVNVEDVTRSLVAAT
                     TSSRFTQPVFWADPGTGISFNVQVQIPEERTQTLEDLGNTPVKSANGGTVLLRTIAKL
                     EPGTAVGTYERYNMVRIASITANLHDIDFGSAIKAVRKAIDEVGPAPDGKTKVDIRGQ
                     VVPFNQLYEGFSSGLVIAIIVIFLLLCANFQSLRLAIVVVSTMPAVLAGVVLALFFTG
                     TTINIQSAMGSIMAVGVAVANAILLVTFADRARLALKGDRRTAAIEGAVSRLRPILMT
                     SFAMIAGMMPLALGAGQTAPLGRAVVGGLALATVATLFFLPAVFALLASKKATSASLD
                     PEDEQSALFEGTTSA"
     gene            complement(5266..5691)
                     /locus_tag="PLP58_16070"
     CDS             complement(5266..5691)
                     /locus_tag="PLP58_16070"
                     /inference="COORDINATES: protein motif:HMM:NF024861.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - endoribonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative toxin-antitoxin system toxin component,
                     PIN family"
                     /protein_id="HRJ10097.1"
                     /translation="MTVCLDTNVMLQARALSHPYGVLLDSYGAGKISLAVSSRILLEY
                     EEILTAKAGEHHWRKLLLFIRTVARRHRNLITIDPWFEFRVIGQDPDDNAFTDCAIAA
                     HADYVITEDRHFAPLANAGYKPQPVTPDEFIARYRGVHV"
     gene            complement(5688..5930)
                     /locus_tag="PLP58_16075"
     CDS             complement(5688..5930)
                     /locus_tag="PLP58_16075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ10098.1"
                     /translation="MTTSQPIPKNLREQLVDSIVAMSEEQLLALHNAILDVEIERLRN
                     ELSEQAEQEQAEGKWDNLQGFIQEYRQRKKSERMKS"
     gene            6000..6983
                     /locus_tag="PLP58_16080"
     CDS             6000..6983
                     /locus_tag="PLP58_16080"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HRJ10099.1"
                     /translation="MKPLLLFTLLTSSALALELPATKPQKGTIHRWITLPGEFAPWQQ
                     VDLKARVAGYIQKITVDKGDVVTAGQTLIEIEVPELQADLIKHRAEVKAAEIEVKRLH
                     EARAKSPDLILPQSVDDAEAKLAIAQAGMERTNALIDLAQIKAPFAGVITERRVDLGA
                     FAAAGGGTLLQLTEVGTLRFQVPVIETETAHLKPGLQVEAKVDALNGAVIKGTLSRLS
                     GQLDTATRTMLIEADFKNDDGRLRPGMFATARIAVEQHDNATIIPVAGLVKEKANSFV
                     FKHVDGKAVKTAVKPGFNDGVNVEVPELKAEDVILLPGTTALTDKQDVTVN"
     gene            7027..8310
                     /locus_tag="PLP58_16085"
     CDS             7027..8310
                     /locus_tag="PLP58_16085"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HRJ10100.1"
                     /translation="MHRLLLLIVCVTTVTAAPLHVDLPTVMKLAGANNDEIQLARARH
                     TETLAESKLAWQRFWPSVTLGAAYRRTDGNVQDIAGAVFDVSKQHYTLGPNIMIDWSP
                     GDMYYSALAAKQKALAAEQLAEKARRDIVTQSTGRYYDLLAAEAALAIIEDDLRLTQD
                     YEKQIGGAVEAGTAFRADLLRVKTQVSREKLAIRQHEERRDLAAAALSEILRLPAETE
                     LRPAKADLVPVRLLDRKTGVASLISQATQHRPEMRAAGAITTAAAWERERARVAPMIP
                     SVQAGYGFGGLGGGFNGDMGNFDETQNVFIGFGWKIGPGGLFDKQRKVIANAQEEAAN
                     LQSAQVKAAIGREVVEAAAKAQSAFDQVSINDEAVEAAQEMVKLAKERQASQLGVVLE
                     YLLAREELTRARQSRVQAVTRFNQAQHELKRAVGR"
     gene            8660..10843
                     /locus_tag="PLP58_16090"
     CDS             8660..10843
                     /locus_tag="PLP58_16090"
                     /inference="COORDINATES: protein motif:HMM:TIGR02074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HRJ10101.1"
                     /translation="MKRKIRAPHKPPPRHSWWRRWLRALVLVCLAACLLAALGAAGVI
                     AYYSRLALRYDLDRLGEMPERTLVLDADGAVLGRMHGENRIVVPLSQVSPHFIKALLA
                     REDSRFYKHGGIDPVGVARAFVRNVREGRVVQGASTITMQLARNSYPDLNDRSFHRKL
                     VEMMLARRIERHWSKDQILEHYVNRIFFGSNLYGIQRASQVYFGKHASQLSLSEAAMI
                     AGIIRSPVRFSPFRNFEGALKERDDVLERMLLTKVVTKEEELEARYADIALKAQPAFQ
                     SQGGYALDVVRRDLDQILEEKEIEDGGLIVYTTLNQQLQIAAEEAVEKRLAAAEELPG
                     YAHPAKAAFDASWDGSGEVSSTPYLQGAVAILDNETGGILALVGGRDYRQSKYNRATQ
                     GNRQIGSTVKPFVYAAAIAGGFLPGTQVEDAPIQPGEIEFADPGWSPKNNDGKFTGAQ
                     TLSTGLVQSRNTMTVRVGNFAGLDRVINVIADAGIGNHLTRTPQVFIGNLGGTPRELA
                     SAFSIFPNDGVRRRTFLIDKVTDKAGNILWSTNVLESVIIPPGSAHLMRRMLAEVMDR
                     GTAAGIRGEHGFKEPAGGKTGTTNDYKDAWFAGYTDRVTCAVWVGLDRPQTIMDQGYG
                     SRLAIPIWADVVKKAAALGYIPAGPRAEPPLQSVQLCRLSSQLATPACQATGTAYEDK
                     LPVEMIPQGWCGAHQGVIARPEMRDTRRQGPAGPGLFDRIRGWFR"
     gene            10991..11066
                     /locus_tag="PLP58_16095"
     tRNA            10991..11066
                     /locus_tag="PLP58_16095"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:11024..11026,aa:Ala,seq:cgc)
     gene            complement(11128..>11309)
                     /locus_tag="PLP58_16100"
     CDS             complement(11128..>11309)
                     /locus_tag="PLP58_16100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ10102.1"
                     /translation="QGFLFSRPLPAEDFAALRDARGRAAILDDLHILFSALIHTPFYT
                     PKRARKSLKRTVSAI"
BASE COUNT         2050 a         3630 c         3548 g         2081 t
ORIGIN      
        1 gtcctatgcg gcgagccgcc cgccggcttc accctccccg gcagcacccg cctgccttcc
       61 cctccgcgcg tgccctccgg cctgcccagc accgtcatgc agcgtcgccc ggacattcgc
      121 gccgcagagc agaacctgcg cgccgccaac gcctccatcg gcgcagccaa agccgagttt
      181 tttcccgcct tccgccttgt cggcaccggc ggtctggaga gtgtcggttc cgagggcttc
      241 atccagggcc gggccaaaac cgccagcatt gggccgcaga tcacccttcc tgtcttccag
      301 ggcggcaggc ttttcggcgg actgcgtgga gccgaggccc gctaccatga atccctggcc
      361 gcctacaagc agacgatcct caccgctctg cgcgaggtgg aggactccat gctcgacgtc
      421 cgcaccctcg gccgccagcg cgccgccgtc gctgccgccg tcgccgccgc gcaggaaacc
      481 gccaaactct cccgcctgcg gtacgatcgc ggcctctcga actacttcga ggtcgtggac
      541 gccgagcgcg tcgtgctcac cgcccgcctc actctcgcgc aactcgacgg ccagcgcctc
      601 ctggccgccg tgcagatgct ccgcgccctc ggcggcggct ggtgaggccg ttcatcagct
      661 ccccttcagc gccgtgatca gcgcgttgag cgtcgccacc acctcctgct gttgcgcctg
      721 ggagtactcc gcgtccggcg tcagctccag cgtgttcacg ccggggatgc cgtgcgcgcg
      781 ctcctggatc aggtcaaaaa gcgcgttgaa ctgcgcgcag cggcgcggac tcgatctcgg
      841 cgctggcggg gggatagtcg gggtcgaaca taaggggcgg ggtgttggtg gacggcgggt
      901 gaagccctca aagctgccac cgtgctgccg gcggctgaca cccttgacaa aatcctccgc
      961 tatgagatca agctggagcg ccagatgtac cgggccatgg accagctcga acgcatccag
     1021 cgcatgcgcc ggggggaact gatacccgcg cccttgtctg tcgctgtctc aggccggggg
     1081 tgagggcaat catgaatttt tgtcaaacga acccatttgc aggcaggctt cctgacgcgg
     1141 aatcgcatga tgaagtgcag gagcaatgtc atttgactct tattacgact gacgtaatgc
     1201 aaccgcatgg aaaccctcgt gatcgccctt tgtggcttcg tcgcggccgg attgacgctg
     1261 ctgtcgggat tcgggctggg cacgctgctg atgccggtgg tgacgttgtt ctttcccgtg
     1321 gagatcgccg tgtccgtgac ggcggtggtg catctggcga acaacctctt caagctcgcc
     1381 ctcgtgggct ggcgggcgga tcgcggcgtg gtgctgcgct ttggcattcc ggccgtcctc
     1441 gcggcatttg cgggcgcgct gacgctgggc tggctggcgc atctgccgcc gctggcggag
     1501 tattcgctca tgggacacac gctggccgtg atgccggtga aactgaccgt cggactgctg
     1561 atcctgggct ttgtggcgat ggagctttct cccgcgatgg cggcctggcg cttcgatgcg
     1621 cggctgctgc cggtcggtgg atgcgtctcc ggctttttcg gcggtttgtc gggcaatcag
     1681 ggcgcgtttc gcagcatgtt cctgctcaaa acgggcctct ccaaggagca gttcatcgcc
     1741 accggcgtgg tgctggcggt gatcgtcgat ctggcgcgga tgccggtcta tggcgtcgcg
     1801 tttttcaagt caggtccgcc ggtggacctg acgctcgtgg tggtggcctg tctggccgcg
     1861 ttcgccggtt cgttcctcgc cgcccggctg ctgaaaaagc tcaccatccg ctcgctccag
     1921 gttattgtcg gcacgctgct catcctggtg gctttcggga tgatcaccgg cgttttgtga
     1981 accctcgttg ccgccgccag attttttgcg catttgatgg aaagaagctt gaatccaagc
     2041 gttttacgcg gcccgtaatc gctccccgat aaaacggcgc ctcccgtccc gctttcgtca
     2101 ctctcccgaa tgaatctcgc acgctttgcc ctccgctatc cctggaccat catcgtcgcc
     2161 gtcgtggccg tggcgctggg agcctggctg gggctgcagc gcatgacgcg cgacattttt
     2221 ccgccgctgg gcatcccgac gatctatgtg gcgcagccgt atggtggcat ggacccgctg
     2281 cagatggagg gctacctgac ctaccgctac gagtatcact tcctctacat cgcgaacatc
     2341 gagcacgtgg agagcaagtc catccagggc gcctcgatca tgaagctgca gttccatccc
     2401 ggcacggaca tgagccaggc gatgtcggag acggtggcgc aggtgaaccg cagccgcgcc
     2461 ttcatgccgc cgggcacggt ggcgcccttc atcatgcgct ttgacgcggg cagcgtggcg
     2521 gtggggcatc tggtgttctc cacggacgat ccgaacatca cgctcaatca gatgcaggac
     2581 caggcgctga acaaagtgcg ccccgccttt gccacactgc cgggcgtgtc cgcgccgccg
     2641 cccttcggcg gcagctcgcg cggcatcgtg gtgaatgtga acccggatcg catgaaggcg
     2701 tatggcttgt cgccggatga catcgtgcag gccattgcga aggggaatcc gatcagcccg
     2761 tccgggaaca tgaacctcga cggcaagtat cccatcgtgc cgacgaacgc gatcgtgtcg
     2821 aatgtgaagg atctggaggc ggtgccgctg cgtcacacgg acaaaggcgc ggtcttcatc
     2881 cgcgatgtcg ccacggtggc cgactccgcc gatgtgacga cttcctacgc gctggcgaac
     2941 ggcaagcgca ccgtctatct gccggtgacg aagcgcgcgg aggcctcgac cttggaggtg
     3001 gtgaagaagg tgcgtgaaag cattcccgaa ttccaaaagc tgctgcctga cggcatcaag
     3061 gtgagctttg agttcgacca gacgccggtg gtgaaccaga gcatccgtga cctggtgaaa
     3121 gaaggcgcgc tgggcgcggt gctcaccggc ttgatggtgc tcgttttcct gcgcgatctg
     3181 cgcaccgcct tcatcgtggt gattaacatc ccgctctcgc tcatggccgc tgccttcggc
     3241 ctgtggatca gcgggcagaa catccacctc atgacgctgg gcggcctggc gcttgcggtg
     3301 ggcatcctgg tggatgaggc caccgtgacg gtggaaaaca tccacacgca tcttgcacgc
     3361 ggaaaaacgc tggcccgcgc cgctctcgac ggcacgctgg agacgaccct gccgcgcctg
     3421 ctggcgatgc tttgcatcct cgccgtgttc attccggcct tcttcatggt tggcgcggca
     3481 caggcgctct tcgtgccgct cgcgttggcg gtgggctttg cgatgttcgc ttcgttcatc
     3541 ctttccagca cgctggtgcc agtgctttcg gtgtggttcc tgcccaagaa cacgaagcat
     3601 gaggaagaga ggctgggatt gctggcgcgg gcttatcaag gtgttgtgaa ggcggcggtg
     3661 acgctgcgct ggctgctggt cccggcttat ctcggcggcg cggtcttgat catcgtgacc
     3721 tttggcccct tcctcggctc ggagattttt ccgcagtcgg acagcgggca gttcgccatc
     3781 cgtttccgcg cgcccagcgg cacgcaggtg ggcatcaccg agggcatcgc gcagaaaatc
     3841 ctcaagatca tcggtgacga ggcaccggtg gagttcagca tcggcatggt gggcgtccac
     3901 aactccagct tcccggtgaa cctcgtgcat ctctggaacg gcggcccgga ggagggctgg
     3961 ctggcggtgc agctcaagaa agatggcagc gtggaggtgc cggagttgca ggaaaaactg
     4021 cgcggcatca tgaaacggga gctgcccgag gtgcgggtgt cctttgagcc gcaggacatc
     4081 gtcagccgcg tgatgagctt tggcacatcc acacccatcg agatcgccgt gagcggcccc
     4141 agcctgcctg cgagcaagga gcacgcggag aagctgctgg cgaagctgaa agaactgccc
     4201 ttcctgcgcg acgcgcagat cgcgcaaacg ctcgacgcgc cgacggtgaa cgtgcagatt
     4261 gaccgcgaac gcgccggatt gctcggcgtg aatgtggagg acgtgacgcg cagcctcgtg
     4321 gccgccacga cttctagccg cttcacgcag ccggtgttct gggccgatcc gggaacgggc
     4381 atcagcttca acgtgcaggt gcaaattccc gaggagcgca cgcagacgct ggaagacctc
     4441 ggcaacacgc cggtgaaatc tgccaacgga ggaactgtgc tgctgcgcac catcgccaag
     4501 ctcgagcccg gcacggccgt gggcacctac gagcgctaca acatggtgcg catcgccagc
     4561 atcaccgcga accttcatga catcgacttc ggcagcgcca tcaaagcggt gcgcaaggcc
     4621 attgatgaag tcggccccgc acccgatggc aagacgaagg tggacatccg cggccaggtg
     4681 gtgcccttca accagcttta cgaaggcttc agcagcggcc tcgtcatcgc catcatcgtg
     4741 atcttcctgc tgctgtgcgc gaacttccag tcgttgcgtc ttgccattgt cgtcgtgtcc
     4801 accatgcctg ccgtgctggc cggtgtggtc ctggccttgt tcttcaccgg caccaccatc
     4861 aacatccagt ccgccatggg ctcgatcatg gccgtgggcg tggccgtggc gaatgcgatt
     4921 ctcctcgtca ccttcgccga ccgcgcgcgt ctcgcgttga aaggcgaccg ccgcacggcc
     4981 gccatcgagg gtgcggtaag ccgtctgcga ccgatcttga tgaccagctt cgccatgatc
     5041 gccggcatga tgccgctggc gctcggcgcg ggtcagactg cgccgcttgg acgtgccgtc
     5101 gttggtggtc tggcactcgc caccgtggcc acgctgttct tcctgcccgc cgtctttgcc
     5161 cttctcgcca gcaagaaggc cacctctgca tcgctcgatc ccgaggacga gcagagcgcg
     5221 ctgtttgaag gcaccacttc cgcctaagcc atgccgtcac tgcgctcaaa catgaacacc
     5281 gcgataccgg gcgatgaact cgtccggcgt gacgggttgc ggcttgtagc cagcgttggc
     5341 aagcggggca aagtgccggt cctccgtgat gacgtaatcc gcgtgggcgg cgatggcgca
     5401 gtcggtgaag gcattatcat caggatcctg gccgatcacg cgaaattcaa accaaggatc
     5461 aatggtaatc agattccggt gccggcgggc aaccgtgcga atgaacaaaa gaagcttgcg
     5521 ccagtgatgt tcgccggcct tagccgtcag gatttcctca tattccagca gaatgcgcga
     5581 cgacacggcc aacgagattt tgccagcccc atagctgtcc agcagcactc cgtaaggatg
     5641 cgacaaggca cgagcctgga gcatcacatt cgtgtcaaga cacactgtca tgatttcatc
     5701 cgctcagatt tttttcgttg gcgatactcc tggatgaagc cttggagatt gtcccacttg
     5761 ccctccgcct gctcctgctc cgcctgttcc gacagttcat tccgcaaccg ctcaatctcc
     5821 acgtccaaga tcgcattatg caaggcaagc aactgctcct ccgacatggc gacgatggag
     5881 tccacaagtt gctcgcgcag atttttgggg atgggctgtg aggttgtcat gccgccaaga
     5941 ttaaccgcac gcattggtct ttccaatttc ttttccacca ccacccgccg acacttttca
     6001 tgaaaccgct cctgcttttc accctcctca cctccagcgc cctggcactc gagcttccgg
     6061 ccaccaaacc gcaaaagggc accatccatc gctggatcac gctcccgggc gagttcgcgc
     6121 cgtggcagca ggtggacctc aaggcccgtg tggctggtta catccagaag atcaccgtgg
     6181 acaagggaga tgtcgtcacc gccgggcaga ccctcatcga aattgaggtg ccggagctgc
     6241 aagccgacct catcaaacat cgcgccgagg tgaaggcggc ggagatcgag gtgaagcgcc
     6301 tgcatgaggc gcgggcgaag tcacccgacc tcatcctgcc gcagtccgtg gatgacgcgg
     6361 aggcaaagct cgccatcgct caggccggca tggagcgtac gaatgcgctc atagacctcg
     6421 cgcagatcaa ggcgcccttc gccggagtca ttaccgagcg ccgagtggac ctcggtgcct
     6481 tcgccgccgc cgggggcggg acgctgttgc agcttaccga ggtcggcacg ctgcgattcc
     6541 aggtgccggt gatcgagact gagaccgcgc acctcaagcc cggcctccaa gtcgaggcga
     6601 aggtggacgc gctcaacggc gcggtcatca aaggcacgct cagccgtctc agcggccagt
     6661 tggacacagc cacgcgcacc atgctcatcg aggccgactt caaaaacgac gatggcaggc
     6721 ttcgtcccgg catgtttgcc accgcgcgca tcgccgtcga gcagcatgac aacgcgacga
     6781 tcatccccgt ggccggtctg gtgaaggaga aggccaacag ctttgtcttc aagcatgtgg
     6841 acggcaaggc ggtgaagacg gcagtgaaac ccggcttcaa cgacggagtg aatgtcgaag
     6901 taccggagtt gaaggccgag gatgtgattt tgctgccggg caccaccgcg ctgaccgaca
     6961 agcaggatgt gacggttaat tgatgattaa tgatcggcta aagcctagac accaactctt
     7021 gctgccatgc atcgtcttct acttttgatt gtctgtgtga caacagtcac cgccgctccc
     7081 cttcacgttg atctgccgac cgtgatgaag ctcgcgggag cgaacaacga tgaaatccag
     7141 ctcgcgcgag ccaggcacac ggagactctg gcggagtcaa agctggcctg gcagcggttc
     7201 tggccgagcg tgacgctggg agcggcttac cggcgcacgg atgggaatgt gcaggacatc
     7261 gcgggcgcgg tgtttgatgt cagcaagcag cactacacgc tggggccgaa catcatgata
     7321 gattggtcgc cgggggacat gtactactcg gctctggcgg ccaagcagaa ggcgctggcg
     7381 gcggagcaac tcgcggagaa agcgcggcgc gacatcgtca cacaatccac ggggcgctac
     7441 tatgatttgt tggccgcgga agcagcgctg gcgatcattg aggacgatct gcgactcacg
     7501 caggactacg aaaagcagat cggcggcgcg gtggaggctg gcacggcctt ccgtgcggat
     7561 ttgctgcgcg tgaagaccca ggtcagccgc gaaaagctcg ccatccgcca gcatgaggag
     7621 cgtcgtgatc ttgctgccgc ggcgctctct gaaattctgc gcctgcctgc tgaaaccgag
     7681 ctgcgcccag ccaaggcgga ccttgtgcct gtgcgtctgc tggatcgcaa gacgggagtc
     7741 gcatccttga tcagccaggc cacgcagcat cgtcccgaaa tgcgcgccgc tggagccatc
     7801 accacggcgg cggcctggga aagggagcgc gctcgtgtgg ctccgatgat ccccagtgtg
     7861 caggccggtt acggattcgg aggactcggc ggcggtttca atggcgacat ggggaacttt
     7921 gatgaaaccc aaaacgtctt catcggattc ggctggaaga tcggccccgg cggccttttc
     7981 gacaagcagc gcaaagtcat cgccaacgcg caggaggagg cggcgaatct gcaatccgcg
     8041 caagtcaaag ccgccatcgg ccgcgaagtg gtggaggctg ccgcaaaagc gcagagcgcc
     8101 ttcgatcagg tcagcatcaa cgatgaggcg gtggaggccg cgcaggaaat ggtcaagctc
     8161 gccaaagagc gccaggccag ccagctcggc gtggtattgg agtatctgct ggcccgcgag
     8221 gagctgaccc gcgcccgcca gagccgggtg caggcggtga cgcgcttcaa ccaggcgcag
     8281 cacgagctga agagggcggt tggcagatag ccttgtcggt catgccatct tgcttgggcg
     8341 cggacactat actgaaggtc atcacctttt gtgaatttgg ttttctccca cagattcagg
     8401 gaggcccaca gattaaggat gagaaagaga aatctgccgg cctccttgaa tctgcgggaa
     8461 atcacaattc atggtgacgc acggcatatt cgtccaaaag tgaggaagcg cttgtctgat
     8521 ggagccagct ccgccggatc atgcaaggct gccggagcgt gtgatggcgc ggcatggcag
     8581 cgctggcgcg tcaacctcgg ctggcattca caaaatttca ggccaatact ttccttgttt
     8641 ccgcgtccaa gcccgcctca tgaaacgcaa aatccgcgct ccccacaaac ctccgccgcg
     8701 ccactcctgg tggcggcgct ggctgcgcgc gctggtcctg gtctgcctgg cggcatgcct
     8761 gctggcggcg ctgggggcgg cgggagtcat cgcctactat tccaggctgg ccctgcgcta
     8821 tgacttggat cgtctcggcg agatgccgga gcgcacgctg gtgctcgacg cggacggcgc
     8881 cgtgcttggc cgcatgcatg gcgagaaccg catcgtggtg ccgctcagcc aggtctcgcc
     8941 gcatttcatc aaggcgctgc tcgcccgcga ggacagccgt ttttacaagc acggaggcat
     9001 tgaccccgtg ggcgtggcgc gcgctttcgt ccgcaatgtc agggaggggc gcgtggtgca
     9061 gggcgcgagc accatcacca tgcagctcgc gcgcaacagc tatcccgacc tcaatgatcg
     9121 aagcttccac cgcaagctcg tcgagatgat gctggcccgc cgcatcgagc gccattggtc
     9181 gaaggaccag atcctcgagc attatgtgaa ccgcattttc ttcggctcga acctctacgg
     9241 catccagcgc gccagccagg tttattttgg caagcacgcc agccagctca gtctgagcga
     9301 ggccgccatg attgccggca tcatccgcag tcccgtgcgt ttttcaccct tccgcaattt
     9361 cgaaggcgcg ctcaaggagc gcgacgatgt gcttgagcgc atgctgctca ccaaggtcgt
     9421 caccaaggag gaggagctgg aggcccgcta cgccgacatc gccctcaaag cccagcccgc
     9481 ttttcaaagc cagggcggct acgctctcga cgtggtgcgc cgcgacctcg atcaaatcct
     9541 ggaggagaag gagatcgagg acggcggact catcgtttac acgacgctca accagcagct
     9601 ccagatcgcc gccgaggagg ccgtggagaa aaggctcgcc gccgctgaag agctgcccgg
     9661 ctacgcgcac ccggccaagg ccgcgttcga cgcctcatgg gacggcagcg gcgaggtgtc
     9721 ctccacgccc tatctccagg gggcggtcgc cattctcgac aatgaaaccg gcggcatcct
     9781 cgccctcgtc ggcgggcgcg actacaggca gagcaaatac aaccgcgcca cccagggcaa
     9841 ccgccagatc ggctccacgg tgaagccctt cgtctatgcc gcagccatcg caggcggctt
     9901 cctgcccggc acccaagttg aggacgcgcc catccagccg ggggagatcg agttcgccga
     9961 tcccggctgg tctccgaaga acaacgacgg caaattcacc ggcgcgcaga ccctcagcac
    10021 cggcctcgtg cagagccgca acaccatgac cgtccgcgtt ggcaacttcg ccggactcga
    10081 ccgcgtcatc aacgtcatcg ccgacgccgg catcggcaac cacctcaccc gcaccccgca
    10141 ggtcttcatc ggcaacctcg gcggcactcc gcgcgagctg gccagcgcct tcagcatctt
    10201 ccccaacgac ggcgtccgcc gccgcacctt cctcatagac aaagtcaccg acaaggccgg
    10261 caacatcctc tggagcacca atgtgctgga gagcgtcatc atcccgcccg gcagcgccca
    10321 cctcatgcgc cgcatgctgg ccgaggtcat ggaccgcggc accgccgccg gcatccgcgg
    10381 cgagcacgga ttcaaagaac ccgccggcgg caagaccggc accaccaacg actacaagga
    10441 cgcctggttc gccggttaca ccgaccgcgt cacctgtgcc gtgtgggtgg ggctggaccg
    10501 cccgcagacc atcatggacc agggttatgg cagccggctc gccattccca tctgggccga
    10561 cgtggtgaag aaagccgccg ccctcggcta catccccgcc ggtccgcgcg ccgagccgcc
    10621 tttgcaaagc gtgcagctct gccggctcag cagccagctc gccactccgg cctgccaggc
    10681 caccggcacc gcttacgagg acaagctgcc tgtcgaaatg atcccccagg gctggtgcgg
    10741 cgcgcatcag ggcgtcatcg cgcgtcccga gatgcgggac acgcgccgcc aggggcctgc
    10801 cggaccgggt ttgttcgacc gcatccgggg atggttccgc tgaagccgcg cgccgccgca
    10861 aagcgccgtc cctcagccat ggcgggccat gtccgcggca tgcgcgcgcg gagcgtggat
    10921 aaaagcgctt gctgtcacca cgccgggccg cataatggcg cgcgttgtcc tggtcgggat
    10981 caacacaaaa ggggccatag ctcagttggt agagcgtctc gttcgcaatg agaaggtcag
    11041 gggttcgaat ccccttggct ccaccactcc cggaaagggg gtggtgcatt gaaaatcaag
    11101 ggttagcgtg gttttttggg gtttttgtca aatggctgaa actgtccttt ttaggctttt
    11161 tctggccctt tttggggtgt aaaagggtgt atggatcaaa gccgaaaaaa ggatgtggag
    11221 atcatccagt atggcggcgc ggccgcgcgc gtcacgcagc gcggcaaagt cctcggccgg
    11281 cagcggccgg gaaaacagga atccctgct
//
LOCUS       DAOMYJ010000175        11147 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Prosthecobacter sp. isolate AMR_MDS_5629
            NODE_1944_length_11147_cov_22.126037, whole genome shotgun
            sequence.
ACCESSION   DAOMYJ010000175 DAOMYJ010000000
VERSION     DAOMYJ010000175.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867850
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prosthecobacter sp. (wastewater metagenome)
  ORGANISM  Prosthecobacter sp.
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Verrucomicrobiaceae; Prosthecobacter.
REFERENCE   1  (bases 1 to 11147)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11147)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.30
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:34:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,742
            CDSs (total)                      :: 4,684
            Genes (coding)                    :: 4,676
            CDSs (with protein)               :: 4,676
            Genes (RNA)                       :: 58
            tRNAs                             :: 55
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11147
                     /organism="Prosthecobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1944_length_11147_cov_22.126037"
                     /isolate="AMR_MDS_5629"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1965333"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(413..1381)
                     /locus_tag="PLP58_16105"
     CDS             complement(413..1381)
                     /locus_tag="PLP58_16105"
                     /inference="COORDINATES: protein motif:HMM:NF015651.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase transmembrane
                     domain-containing protein"
                     /protein_id="HRJ10103.1"
                     /translation="MKKALVIVLKIAVTTVLLGMIFREHQFTGEILPHLRAMGANWPW
                     ALAGLACAGLAVLFAALRWQALLAGQDQRVPPGRVARVTFEAAFFNITSLGALGGDAW
                     RVIALLRRNGAQKLPVMASIMLDHLVGLVSVSIVFLGCGWLVREKLEQLSPEADAVLR
                     GFAVFMAGSLLGILVSVLSFTPRIYAWGESKLPWLLGWPPLKKFGQACDALRHAWRWS
                     LLALGASLVLYLFHFLVFYCGIRAVGGTAPFTGFMAAMPVIDMAAGLPVSVSGLGVRE
                     KTFEALVGGLAGLPASKAVAASLAGWLMNVCWGLAGGLLFVRGRGD"
     gene            complement(1391..1645)
                     /locus_tag="PLP58_16110"
     CDS             complement(1391..1645)
                     /locus_tag="PLP58_16110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007414852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantetheine-binding protein"
                     /protein_id="HRJ10104.1"
                     /translation="MNLRQRIKEVMASELMLEVAAGDITDDGPLFGPEGIGLDSVDAL
                     QLVVAIEKHFKLKISDQTKAREILHSVDSIAKAIEEAGLG"
     gene            complement(1676..2410)
                     /locus_tag="PLP58_16115"
     CDS             complement(1676..2410)
                     /locus_tag="PLP58_16115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007414851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HRJ10105.1"
                     /translation="MSDQRRILITGANGVLGCVVARHFLERDPDCRVFLGVREKRDDA
                     EALASCHAGRAFLCPLEVTRAEDWQEAVARIEAEAGPLSVLVNNAGCHDDALLAHMTG
                     GQWARVLDANLNAVFLGCQAALQPMLRERWGRIINISSLSALHSPAGQTNYAAAKAGV
                     IGLTQSLAKETARLGITVNALCPGHIEGAPPAGWSVEQLQAARRQTPMRRFARPEEIA
                     AVVFFLASREASYMTGAALKLDGALV"
     gene            complement(2420..3778)
                     /gene="tig"
                     /locus_tag="PLP58_16120"
     CDS             complement(2420..3778)
                     /gene="tig"
                     /locus_tag="PLP58_16120"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR00115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005854 - nascent polypeptide-associated
                     complex [Evidence IEA];
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0051083 - 'de novo' cotranslational protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HRJ10106.1"
                     /translation="MNINVEHQPNCRAVAHIRVPAAEVSKQRSAITSYYASQVRLPGF
                     RPGKAPAAAVRKRYSADIEAELEKQLINDGLRQAIKNEGLEVLNILSVTDKMVHDTDQ
                     SFSFSAEMSLAPKFELPEYKGIPVKLPRVEVTDADVDHDILHLRERHASFQDVERGAQ
                     NGDAVVLTATGSLDGEPLAETMPDAPQHLREIKENWFLLDDEEDFLPGFYAGLNGIRK
                     DETRTLTIPLPEDFPYEPLRQKNIEFTVNCKGVKEKKVPELDGDLLKKIGGEELTVEM
                     LRGEVREAIRRRREEARENGKGNQVIAHIFEKVEFDLPQEIVNREAQRRTNEMAMRAM
                     QQGLSHEELLKHQDEILGSATHQARQSIKVSFILEQVAQKENLEVTQQQLMHALANLA
                     ARQNKPVKKFMAEAQKSGLISSLQEDLLLQNAMQFLKDNAIVEETDPEPEVCEQHPAA
                     AA"
     gene            complement(3960..5420)
                     /locus_tag="PLP58_16125"
     CDS             complement(3960..5420)
                     /locus_tag="PLP58_16125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009965585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1501 domain-containing protein"
                     /protein_id="HRJ10107.1"
                     /translation="MTLLHEHLSHLTRRSFLGDVSRGIGGIALGSLLHQEGLRAASDA
                     PRIGLPGFPHFAPKARRVLCLFQSGGLSHVDLFDDKPMLHKHAGQEIPPSIKGTQRLT
                     GMTSGQSAYPVVPPLMAGRRCGKHGTWISDLLPHIQTIADDICLVKSLHTEAINHDPG
                     ITYLNTGNQLPGYASMGAWVSYGLGTENQNLPAFIAMVSQGTGKNPGQPIFSRLWGSG
                     FLPSSHQGVGLRPGANPVLYLQNPPGVERPQRRALLDDLVRLNQMRADELGDPETLTR
                     IKAYEMAYRMQASVPDLTDISGESAATLEAYGPDVRKPGSYAANCLLARRLLERDVRF
                     VQLFHRGWDQHIAISRQLPNQCHDIDQPTAALIKDLKSRGLLEDTLVVFATEFGRTTF
                     SQGKLGDPGMGRDHHGRCFSIWLAGGGVKPGFEYGSTDDFCYNIAENPVHIRDLHATI
                     LHCLGIDHARFSHKFRGLNVRLTGVEESHVVRDILL"
     gene            complement(5440..8571)
                     /locus_tag="PLP58_16130"
     CDS             complement(5440..8571)
                     /locus_tag="PLP58_16130"
                     /inference="COORDINATES: protein motif:HMM:NF019223.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PSD1 and planctomycete cytochrome C
                     domain-containing protein"
                     /protein_id="HRJ10108.1"
                     /translation="MKRTLLSIPLTACLCSFGHGAAAIDYNRDIRPILSENCFSCHGF
                     DEKARKAKLRLDMAEDAHADKDGVIAFKPGDLKNSEAWQRIISDDEDDMMPPPESHLK
                     LSAADKEKIRQWIGEGAKYAAHWAFISPLRTVIPDGTSNAIDALVRKRLAEEGMKPSP
                     AADPAMLMRRVSLDLTGLPPGAEEVEAFVKDASPEAYEALVDRLLKSPHFGERMALEW
                     LDAARYADTNGFSIDGGRSMWLWRDWVIQAFNDNKPYDRFLIEQLAGDLLPDRTEADL
                     IASGFQRNNMVTHEGGTIPEENLTNYNADRVKTLGEAVLGLTLACAQCHDHKFDPITQ
                     KDYFRMFAFFNSLSDRGLDGNGGVNPGPFISAKTVLKTDEAPALQKQISTLRQRLATP
                     HAKIVRAWENRERARLDKRGTAFQTHPTRILKVSTPNRGNGFEMEGQNGVRLPQVAAM
                     AAYDVSMELPKISAPITGLRVVFHPVPDLPGGGWGSGPQEGRAPAKAKAKTAPKEAAP
                     AHKGSFVLTSIAATAGSIANDQINLHKLEEIAHVTANSWDVKYPPAACLDPRNESGWS
                     PDPATEGPVRLTATFARPLSASDTPFLTTQLVFGHGKSLIPGHFEIFVITGEDDDSAL
                     PADILAILGTAEAKRSPQQKAALWGYAAAHADEMRRTRVDLANLEERLAALTQPHPTM
                     VMDVAAKPRDTFILNRGDYAQPTVKVSAGTIEALPPMPDGAGQDRLGLAQWITMKGNP
                     LTARVAVNRFWKMLFGTGIVATAADFGMQGEWPSHPDLLDWLAVEFMDSGWDVKRLLK
                     LIVMSDTYRQTSAATPEMLERDPQNRLLARGPRFRLPAELLRDAALKTSGLLVPRIGG
                     PSVNPYTPGDLWREVSHYGSTPATAQTFVQDHGEKLYCRSLYTFWKRTAAPPNMTAFD
                     APNREVCTIDRGNTTTPLQALVTLNDPQFVEAARAFAQRALHQPGDDAARLRWAFAEA
                     TSRQPKAAELGVLLAALKRERQHYNAKPQAAAQALRVGESPRDGKLPAIEHAAWMQVA
                     ALILNLSESVTRN"
     gene            complement(8676..>11147)
                     /locus_tag="PLP58_16135"
     CDS             complement(8676..>11147)
                     /locus_tag="PLP58_16135"
                     /inference="COORDINATES: protein motif:HMM:NF019223.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1549 and DUF1553 domain-containing protein"
                     /protein_id="HRJ10109.1"
                     /translation="LAAEGLAPSAPAAPETLARRLHLDLTGIPPVPAEVEHFVKDHAA
                     DAQGAVERLADRLLNSPDYGVKWARHWLDLARYADSHGFQRDDLRQIWGWRDWVVDAL
                     NADKPFDEFSIEQIAGDLLPNATPEQIIATGFHRCTPTNVEAGTEPEESRINQVIDRV
                     NTTGAVWLGSTLECAQCHNHKYDPFTQQDYYRLLAYFNNTEREAERANPKVPGSIAFK
                     GSPMEMRDTTQEQERAALQKQMGEMDARISAHTKRGGRPANKEKTASAITALKPLRVD
                     TESASEAETRDDASILFIGEAPDGDTYTLELDLPTTPVHGILIEVLTDPSLPGDGPGR
                     GDATRPNFVLQELHAELLTPGKDKTGGVPLKFARARASFSQARFDVAGFIDEDPATGW
                     AINPQFHKPHWAALAFEKPLTLEPGSRLGLRLVQHFGGGRVIGRLRVSAFTATVEEHL
                     PEPVDAGDDKAAKDPVLARMEAQRKALQRKLDALKPATSEVMRELPDPRMTTLFKRGE
                     YTNPGDPVTAAVPALFEKQPEGAPNRLTLARWLVSRDNPLAARVTVNRIWNEIFGRGI
                     VATVEDFGIKGTPPTHPELLDWLAVEFMDQGWSLKKLLRLIVTSQTYQQSSHAPANAA
                     AQDKDPENLLLWRMSRFRLDAEGIRDNALAIAGLLNPKQGGPPIYPPQPDGLWAKVGG
                     QQYKYEVSPGAEKHRRGLYVVLKRGAPYPSFVNFDSGPRMACVVKRHRSNTPLQALNL
                     LNDPVFVEAAQAFSKRIITEKPGANAEQRIRHGFRLALSRDPTPTELAALHALWQKQY
                     VKGETAAFEAVALALLNLDETISKE"
BASE COUNT         1803 a         3377 c         3888 g         2079 t
ORIGIN      
        1 cccccaaaga ccgctcggag gagttttgac acagtctcgc cagaatgcgg ggccttccga
       61 tcacgtcgca agcccacccc agccctcgtt ctggcgaacg agcctacgac gggcggcagg
      121 cgcagccgcc agactgcggg gatcgaaaag ccccgatgct ctcccaacct cctcgttctg
      181 gcagacgagc ctgcggacag tgcctggtgc ggagcgcatg cgtcccgcgt gccgtttacc
      241 cgcgtcttca cgacaatccg tggctttgaa cttccgtcat cccatctgtc gtggccgacc
      301 gagcgcagcc ctcagggaca ggctgtggcg gggcgccgca gccgacatgc gggccgtgtt
      361 gctccgttct cgaggagcac gcgcggtgag ctggcatggc gaaactgagg tgtcagtccc
      421 ccctcccgcg cacgaagagc aggccgccgg ccagccccca gcagacgttc atgagccagc
      481 cggccaggga ggctgcgacg gccttggacg cgggcaggcc ggcgagtccg ccgacgaggg
      541 cctcgaaggt cttttcccgc acgccgagac cggagacgga gacgggcagc ccggcggcca
      601 tgtctatgac aggcatggcg gccatgaacc cggtgaaggg tgctgtgccg cccacggcgc
      661 ggatgccgca gtagaagacg aggaagtgga agaggtagag caccagggag gcgccgaggg
      721 cgagcaggga ccagcgccag gcgtggcgca gcgcgtcgca ggcctggccg aattttttca
      781 gcggcggcca gcccagcagc cagggcagct ttgactcgcc ccaggcatag atgcgcgggg
      841 tgaaggagag cacggaaacg aggatgccga ggagggagcc ggccatgaag acggcgaagc
      901 cgcgcagcac cgcgtccgcc tccgggctga gctgctccag cttttcccgc accagccagc
      961 cgcagccgag aaaaacgatg gagacggaga caaggccgac gaggtggtcg agcatgatgg
     1021 aggccatgac gggcagcttc tgcgcgccat tccttcgcag cagggctatg acgcgccatg
     1081 cgtccccgcc gagcgcgccg agggaggtga tgttgaaaaa ggctgcctcg aaggtgacgc
     1141 gcgccaccct gcccggcggc acgcgctggt cctggccggc cagcaacgcc tgccagcgca
     1201 gcgcggcgaa gaggacggcc agcccggcgc aggcaagccc ggcgagcgcc cagggccagt
     1261 tggcacccat ggcgcgcagg tgcggcagga tttccccggt gaactggtgc tcgcggaaga
     1321 tcatgccgag gagcaccgtg gtgacggcga tcttcaggac gatgaccagg gcttttttca
     1381 tgcccttggc tcagcccagc ccggcttcct cgatggcttt ggcgatgctg tccacgctgt
     1441 gcaggatctc gcgcgctttt gtctggtcgc tgatcttgag cttgaagtgc ttttcgatgg
     1501 ccaccacgag ctggagcgcg tccacactgt ccagaccgat gccttcggga ccgaagagcg
     1561 gtccgtcgtc ggtgatgtcc ccggcggcga cttccagcat cagctcgctg gccatgactt
     1621 ctttgattcg ttggcggagg ttcattcggg gggaaaaaaa aggacggccc gccgctcaca
     1681 ccagcgcgcc gtccagcttc aaggcggcgc cagtcatata gctggcctcc cggctggcaa
     1741 ggaagaaaac cacggcggca atctcctccg gccgggcaaa gcggcgcatg ggtgtctggc
     1801 gccgcgcggc ctggagctgc tccacgctcc agccggcggg gggcgctccc tcgatgtggc
     1861 ccgggcagag ggcgttgaca gtgataccga ggcgggcggt ctccttggcc aggctctggg
     1921 tgaggccgat gacgcccgcc ttggcggcgg cgtagttggt ctggccggcg ggggagtgca
     1981 gggcgctgag ggaggagatg ttgatgatgc ggccccagcg ctcgcgcagc atgggctgga
     2041 gcgcggcctg gcagccgagg aagacggcgt tgaggttggc gtcgagcacg cgcgcccatt
     2101 ggccgccggt catgtgggcg agcagggcgt cgtcatggca gccggcattg ttgaccagca
     2161 cgctgagcgg cccggcctcc gcctcgatcc gcgccaccgc ctcctgccag tcctccgctc
     2221 tggtgacctc caatgggcag agaaaagcgc gcccggcgtg gcaggaggcc agcgcctccg
     2281 catcgtcgcg cttttcacgc acaccgagga agacgcggca atccgggtcg cgctccagga
     2341 agtggcgcgc caccacgcac cccaggacgc cgttcgcgcc ggtgatgagg atgcggcgtt
     2401 ggtcggacat gcgctggggt cacgcggcgg cggcggggtg ctgctcgcaa acttcgggct
     2461 cggggtcggt ctcttcgacg atggcgttgt ccttgaggaa ctgcatggcg ttttgcagca
     2521 gcaggtcttc ctgcaggctg ctgatgaggc cgctcttctg agcctcggcc atgaattttt
     2581 tcaccggctt gttctggcgg gcggccagat tcgccagggc atgcatgagc tgctgctggg
     2641 tgacctccag attctccttc tgcgccacct gctcgaggat gaagctgacc ttgatgctct
     2701 gacgcgcctg gtgggtggcg ctgccgagga tctcgtcctg gtgcttgagc agctcctcat
     2761 ggctgaggcc ctgctgcatg gcgcgcatgg ccatctcgtt ggtgcggcgc tgggcctcgc
     2821 ggttgacgat ctcctgcggc aggtcgaact ccaccttctc aaagatgtgg gcgatgacct
     2881 gattgccctt cccgttctcg cgcgcttcct cccggcggcg gcggatggcc tcgcgcacct
     2941 cgccgcgcag catttccacg gtgagttcct cgccgccaat cttcttgagc aggtcgccgt
     3001 cgagctcggg cacctttttc tccttcacgc ctttgcagtt gacggtgaat tcgatgttct
     3061 tctggcgcag cggctcgtag gggaagtcct ccggcagcgg gatggtcagg gtccgcgtct
     3121 cgtctttgcg gatgccgttc agtccggcat agaagccggg gaggaaatcc tcctcgtcgt
     3181 cgagaaggaa ccagttctcc ttgatctccc gcaagtgctg gggcgcgtcc ggcatggttt
     3241 cggccagggg ctcgccgtcc aggctgccgg tggcggtgag caccacggcg tcgccgtttt
     3301 gcgctccgcg ctccacatcc tggaaggagg cgtggcgctc gcgcaggtgc aggatgtcat
     3361 ggtccacatc ggcgtcggtg acctccacgc gcggcagctt caccgggatg cccttgtatt
     3421 caggcagctc gaatttcgga gccaggctca tctcggcgga gaagctgaag ctctggtccg
     3481 tgtcgtgtac catcttgtcg gtgaccgaga ggatgttcag cacttccagg ccctcgtttt
     3541 tgatggcctg gcgcaggccg tcgttgatca actgcttctc cagctcagcc tcgatgtccg
     3601 cgctgtagcg cttcctcacc gcggcagcgg gtgccttgcc ggggcggaaa ccgggcaggc
     3661 gcacctggct ggcatagtaa gaggtgatgg cgctgcgctg tttgctgacc tcggcggcgg
     3721 gcacacgaat gtgcgccacg gctcggcagt tgggctggtg ttcgacgttg atgttcatgg
     3781 gggtgggaaa actcgccttc cagggggcgc gcggatgacc ggagcctcgg agggggtgtc
     3841 aggctagagc agccccgccg ccttgacaac cctggaagcg attttcaaaa ctccccgccg
     3901 gcccgccggg cgggcaaagc cccgccgcag cccgaaacac ccgccgccga atccgtaggt
     3961 caaagcaaga tgtccctcac cacatggctt tcctccacgc cggtgaggcg gacattgagg
     4021 ccgcggaact tgtggctgaa gcgggcgtga tcaatgccca ggcagtgcaa aatggtggcg
     4081 tgcaggtcgc ggatgtggac ggggttttcg gcgatgttgt agcagaagtc atccgtggag
     4141 ccatattcga agccgggctt cacaccgccg ccagccagcc agatggaaaa gcagcgccca
     4201 tggtggtcac gacccatgcc ggggtctccg agcttgccct ggctgaaggt ggtgcgacca
     4261 aactcggtgg cgaagacgac aagcgtgtcc tccagcaagc cgcgtgattt gagatccttg
     4321 atgagcgcgg cggtgggctg gtcaatgtca tggcattggt tcggcaactg gcgtgagatg
     4381 gcgatgtgct ggtcccagcc gcgatgaaaa agctgcacaa aacgcacatc ccgctccagc
     4441 aggcggcggg cgagcaggca gtttgcggca tagctgccgg gcttgcgcac atcggggcca
     4501 taggcctcca gcgtggcggc ggactcgccg ctgatgtcgg tgagatccgg cacgctggcc
     4561 tgcattcgat aagccatctc gtaggccttg atgcgtgtca gtgtctcggg gtcgcccagc
     4621 tcatcggcgc gcatttggtt gagccggacg aggtcgtcga gcagggcgcg gcgctgcggg
     4681 cgttccacgc cgggcgggtt ttgcagatag agcaccggat tggcccccgg ccgcaggccc
     4741 actccctgat gcgaggaagg caggaaaccg ctgccccaga gacgcgagaa aatgggctgg
     4801 ccgggatttt ttcccgtgcc ctgggaaacc atggcgatga aggccggcaa gttttggttc
     4861 tcggtgccga gcccgtagct gacccaggca cccatgctgg cataaccggg cagttggttc
     4921 cccgtgttca gataagtaat gccgggatcg tggttgatgg cctcggtgtg caggcttttc
     4981 acgaggcaaa tgtcatcggc gatggtctgg atgtgcggca gcagatcgct gatccaggtg
     5041 ccgtgcttgc cgcagcgcct gcctgccatg agcggtggca ccacaggata ggcggactga
     5101 ccgctggtca tgccggtgag acgctgagtg cctttgatgc tcggcgggat ttcctgcccg
     5161 gcgtgcttgt gcagcatggg cttgtcatca aagaggtcca catgagaaag gccgccactc
     5221 tggaaaaggc agagcacgcg cctggccttc ggcgcgaaat gcgggaagcc gggcaggcca
     5281 atgcgcggcg cgtcagaggc ggcgcgcagg ccttcctgat gcagcaggct gcccagcgcg
     5341 atgccaccga tgccgcgtga aacatcgccg agaaagctgc ggcgggtgag gtgggagagg
     5401 tgttcgtgga gaagggtcat gttgggccaa atcaatgttt caattccgtg tcacgctttc
     5461 gctgaggttg agaatgagtg cggcgacctg catccaggcg gcgtgctcga tggcggggag
     5521 tttgccatca cgaggggatt caccgactcg cagtgcctgg gcggcagctt gcggtttggc
     5581 gttgtaatgc tggcgctcgc gtttgagcgc ggctagcagc acgcccagtt cggcggcttt
     5641 gggctggcgt gaggtggctt cggcaaaagc ccagcgcagc cgcgcggcat catcgccggg
     5701 ctgatgaagc gcgcgctgcg caaaggcgcg cgcggcttcg acgaactgcg ggtcattcag
     5761 ggtgacgagc gcttgcagag gtgtcgtggt attgccgcgg tcaatggtgc agacctcgcg
     5821 gttgggcgcg tcgaaggccg tcatgttggg cggggcggcg gtgcgtttcc agaaagtgta
     5881 gagactgcgg cagtagagtt tttcaccgtg atcctgcaca aaggtctgcg cggtggccgg
     5941 cgtgctcccg tagtggctga cctcgcgcca gagatcgccg ggagtgtagg ggttgacact
     6001 gggaccgccg atgcgcggca cgagcaggcc gctggtcttc agcgcggcat cgcgcagcaa
     6061 ctccgcggga aggcggaaac gcggaccacg ggcgagcagg cggttttgcg ggtcgcgctc
     6121 gagcatttcc ggcgtggcgg cggaggtctg gcggtaggtg tcgctcatga cgatgagctt
     6181 gaggagacgc ttcacatccc agccgctgtc catgaactcg accgccagcc agtcgagcaa
     6241 gtccgggtgg cttggccact cgccctgcat gccgaagtcc gccgctgtgg ccacgatgcc
     6301 ggtgccgaag agcattttcc aaaatcggtt caccgccaca cgggctgtga gcgggttgcc
     6361 tttcatcgtg atccactgcg cgagtccaag ccggtcctgc ccggcaccat ccggcatggg
     6421 cggcagcgcc tcgatggtgc ccgcgctgac tttcaccgtg ggctgcgcat agtcgccacg
     6481 gttcaggatg aaagtgtcac gcggctttgc ggccacatcc atgaccatcg tcggatgcgg
     6541 ctgcgtcaag gcggcgagcc tttcctccaa attcgccaag tccacacgcg tgcgccgcat
     6601 ctcatcagca tgagccgccg catagcccca aagcgccgcc ttctgctgcg ggctgcgctt
     6661 tgcctcggcg gtgccgagga tggcgaggat gtccgctggc agggcgctgt cgtcatcctc
     6721 acccgtgatg acgaaaatct cgaagtgccc agggatgagg cttttgccat ggccaaagac
     6781 gagctgcgtg gtaaggaagg gtgtgtcgct ggcgctcagc ggcctggcaa aagtggccgt
     6841 caagcgcact ggtccctcgg tggcaggatc gggcgaccag ccgctttcgt tgcgaggatc
     6901 gagacaggcg gcgggcgggt atttcacgtc ccaggagttg gctgtgacgt gggcgatttc
     6961 ctccagcttg tgcaggttga tctggtcatt ggcgatactg cccgccgtgg ccgcgatgga
     7021 ggtgagaacg aagctgcctt tgtgcgcggg agccgcctct ttcggggcgg tcttggcctt
     7081 ggccttcgct ggcgcgcgcc cttcctgcgg gccgctgccc cagccaccgc cgggcagatc
     7141 gggcacagga tgaaagacca cgcgcagacc ggtgatggga gcgctgattt ttggcagctc
     7201 catggacaca tcataggctg ccatggccgc cacttggggc aggcgcacgc cgttttgccc
     7261 ctccatctcg aagccatttc cccggttcgg tgtggagact ttcaggatgc gggtcgggtg
     7321 cgtttggaaa gccgtgccgc gcttgtcgag cctggcacgt tcacggttct cccatgctct
     7381 tacaatcttg gcatgaggcg tggcgagcct ttgccgcagc gtggagattt gcttctgcaa
     7441 agcgggcgcc tcatcggtct tgagcacggt tttggcactg atgaagggtc ccggattcac
     7501 cccgccgttg ccatcgagcc cgcgatcact gagcgagttg aagaaggcga acatccggaa
     7561 gtagtccttc tgtgtgatgg gatcgaactt gtgatcatgg cactgcgcgc aggccagtgt
     7621 gaggcccagc accgcctcgc cgagcgtctt cacgcggtcg gcattgtaat tggtgaggtt
     7681 ttcctccggg atggttccgc cctcgtgcgt gaccatgttg ttgcgctgaa agccggaggc
     7741 gatgaggtcc gcctcggtgc ggtcgggcag caggtcaccc gcgagctgct cgatgagaaa
     7801 tcggtcgtag ggcttgttgt cgttgaaggc ctggatcacc cagtcgcgcc acagccacat
     7861 gctgcgtccg ccatcaatcg agaagccgtt cgtgtccgcg tagcgcgccg cgtccagcca
     7921 ttccaaagcc atgcgctcgc cgaagtgggg agacttcagc agacgatcca ccagcgcctc
     7981 gtaagcctcc gggctcgcgt ctttgacaaa agcctccacc tcctccgcgc ccggcggcag
     8041 gccggtgaga tcgagcgaca cccgccgcat cagcatcgcc gggtcggctg ccggcgaagg
     8101 tttcatgcct tcctccgcca ggcgcttgcg cacgagtgca tctatggcgt ttgaggtgcc
     8161 atcgggaatg accgtacgca gcggggagat gaaggcccaa tgcgccgcat acttcgcacc
     8221 ctccccgatc cattgccgga tcttctcctt gtctgccgcg ctgagcttga ggtgcgactc
     8281 cggcggcggc atcatgtcgt cctcatcgtc gctgatgatg cgctgccagg cctcgctgtt
     8341 tttcaggtca cccggcttga aggcaatgac accatccttg tccgcatgag cgtcctctgc
     8401 catatcaagc cgcagcttcg ccttgcgcgc cttctcatcg aaaccgtggc aggagaagca
     8461 gttctccgag agaatgggcc ggatgtcgcg gttgtaatca atcgcagccg ctccgtgacc
     8521 aaaggagcac aagcatgcgg ttagcgggat gctgagaagg gttcgtttca tgaaactgga
     8581 gaaaatgagg acacggaatg cggccggagg gggcctgggg gcaagtcttt cgagttggaa
     8641 aataaaggcg tggatcggtg cccggcactt tccgctcact ccttcgaaat cgtctcgtcg
     8701 aggttcagca aagcgagcgc cacagcctcg aaggcggcgg tctcgccttt gacgtattgc
     8761 ttttgccaga gcgcgtgaag agcggcgagt tcggtgggtg ttgggtcgcg gctgagggcg
     8821 aggcggaagc catggcggat gcgttgttcg gcgttggcgc cgggcttctc agtgatgatg
     8881 cgtttgctaa aggcttgcgc agcttcgaca aaaacggggt cattgagcag gttgagcgct
     8941 tgcagaggag tgttcgagcg gtggcgcttc accacgcagg ccatgcgcgg gccggagtcg
     9001 aagttcacaa agctcggata cggcgcgccg cgcttgagca cgacgtaaag gccgcgccgg
     9061 tgcttctccg cgccggggct gacttcatac ttgtattgct ggccgccgac tttggcccag
     9121 aggccgtcgg gctgtggggg ataaatgggc gggccgcctt gcttggggtt gagcaggccg
     9181 gcgatggcga gcgcgttgtc gcggatgccc tcggcgtcga ggcggaagcg ggacatgcgc
     9241 caaagcagca ggttttccgg gtctttgtcc tgcgctgcgg cgttggcggg tgcatggctg
     9301 ctctgctggt aagtctgcga ggtgacgatg agccgtagca gctttttcag gctccagccc
     9361 tggtccatga attcgaccgc gagccagtcg agcagctcgg gatgcgtggg cggcgtgcct
     9421 ttgatgccga agtcctcgac agtggccacg atgccgcgcc cgaagatttc attccagatg
     9481 cggttcacgg tgacacgggc ggcgagtgga ttgtctcggc tgacgagcca gcgcgcgagg
     9541 gtgagccggt tcggagcgcc ctcgggctgc ttttcaaaaa gggcgggcac ggcggcggtc
     9601 acggggtctc cggggttcgt gtattcgccg cgcttgaaga gggtggtcat gcgcggatcg
     9661 ggcagctcgc gcatgacttc ggaggtggcg ggcttgaggg cgtcgagctt gcgctggagc
     9721 gccttgcgct gcgcctccat gcgcgccaac acggggtctt tggcggcttt gtcatccccg
     9781 gcatccaccg gctccggcag gtgctcctcg accgtggcgg tgaaggcgct gacgcgcagc
     9841 ctgccgatga cgcgcccgcc gccgaagtgc tgcacgagcc tcaggccaag acgcgagcca
     9901 ggctccaggg tgagcggctt ttcgaaggcc aacgccgccc agtgcggctt gtgaaattgt
     9961 ggattgatag cccagccagt ggcggggtct tcatcaatga agcccgccac atcaaagcgc
    10021 gcctgggaga aactggcgcg ggcgcgggcg aacttcagcg gcacgccgcc ggtcttgtcc
    10081 ttgccgggcg tgagcagctc ggcgtgaagt tcttgcaaga caaagttcgg ccgagtggca
    10141 tcgccgcgcc cggggccgtc gcctggcagg gagggatcgg tgagcacctc gatgaggatg
    10201 ccgtggacgg gcgtggtcgg tagatccagt tcgagcgtgt aagtgtcgcc atcgggcgcc
    10261 tcgccgatga agaggatgct ggcatcgtcg cgcgtctcgg cctcggaggc ggattcggtg
    10321 tccacgcgca ggggcttgag cgcggtgatg gcggaggcgg ttttttcctt gtttgcagga
    10381 cgcccgcccc tcttcgtgtg cgcgctgatg cgggcgtcca tctcccccat ctgcttttgc
    10441 aaagcggcgc gctcctgctc ctgggtggtg tcgcgcatct ccatgggcga gcctttgaag
    10501 gcgatggagc ccggcacctt ggggttggcg cgctcggcct cgcgctcggt gttgttgaag
    10561 taggccagca gccggtagta gtcctgctgg gtgaagggat cgtacttgtg gttatggcac
    10621 tgcgcgcact ccagcgtgct gcccagccac acggcgccgg tggtgttgac gcggtcaatc
    10681 acctggttga tgcggctctc ctccggctcg gtgcccgcct ccacattcgt cggcgtgcag
    10741 cggtggaaac cggtggcgat gatctgctcc ggcgtggcat tgggcaggag gtcgccggcg
    10801 atctgctcga tgctgaactc atcaaaaggc ttgtcggcat tgagcgcgtc caccacccag
    10861 tcacgccagc cccagatttg ccgcaggtca tcgcgctgaa aaccgtgcga gtcggcgtaa
    10921 cgggcgagat ccagccaatg ccgcgcccat ttcacaccgt aatccgggga gttcagaagt
    10981 cggtccgcga gccgctccac cgcgccctgg gcatcggcgg catggtcttt aacaaagtgc
    11041 tccacctccg ccggcacggg agggatgccg gtgaggtcga ggtgcagccg gcgcgcgagt
    11101 gtctcaggcg cggcgggcgc gctcggtgcc aggccctcgg cggcgag
//
LOCUS       DAOMYJ010000176        11131 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Prosthecobacter sp. isolate AMR_MDS_5629
            NODE_1952_length_11131_cov_21.233207, whole genome shotgun
            sequence.
ACCESSION   DAOMYJ010000176 DAOMYJ010000000
VERSION     DAOMYJ010000176.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867850
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prosthecobacter sp. (wastewater metagenome)
  ORGANISM  Prosthecobacter sp.
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Verrucomicrobiaceae; Prosthecobacter.
REFERENCE   1  (bases 1 to 11131)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11131)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.30
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:34:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,742
            CDSs (total)                      :: 4,684
            Genes (coding)                    :: 4,676
            CDSs (with protein)               :: 4,676
            Genes (RNA)                       :: 58
            tRNAs                             :: 55
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11131
                     /organism="Prosthecobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1952_length_11131_cov_21.233207"
                     /isolate="AMR_MDS_5629"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1965333"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..185)
                     /locus_tag="PLP58_16140"
     CDS             complement(<1..185)
                     /locus_tag="PLP58_16140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ10110.1"
                     /translation="MKNLLLVLIGLATLGSVLALAWVLGTPAAPDPRVAQLEAELKEA
                     RRTIATLKRDLEKRELP"
     gene            475..5577
                     /locus_tag="PLP58_16145"
     CDS             475..5577
                     /locus_tag="PLP58_16145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009959973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1549 domain-containing protein"
                     /protein_id="HRJ10111.1"
                     /translation="MTKSLLPLLALLTALPAFAKEEAADPNKPVSFYKDIRPILQANC
                     TGCHQPAKAKGDYVMTDFAKLLAGGEEGDAVIPGKPEASNILKVSTPGPDGKAEMPPK
                     GDSLHATQLALIQKWITEGAKDDTPASALAHFDMNNPPVYVTPPAVTSLEYSPDGKSI
                     AVAGYHEVLIHKADGSGIEARLVGLSERVQKLAWSPDGKKILVTGGSPARMGEIQIWD
                     VAARKLELSKSLTFDTLYGASWSPDGKFIAVGCGDNGLRAFEAASGKEVVFMGGHSDW
                     VLDTVWGVDGKHLVSCGRDMSVKLTEVETQRFIDNITSITPGALKGGVQALARHPQRN
                     EILIGGTDGVPAIYRMERITKRVIGDNANLIRQFPAMKGRIFGVAFAPDGKRIAAGAS
                     HEGTGAVNIYSSEYDSAMPDAVKKIVETVNGKGKELDDWITKDVKLLSSTPIATGGVF
                     SVSFSPDGMTVAAAGQDGRVKLIEAATGKIAKEFLSVPLVEDKALAASDVIADDSVRA
                     TAKKDDKVETLVPGLTIASIEVEPKSIAIGKPNEYAQLLVTATMNDGSKADITRMAKL
                     SSSADAASVNARGLVRPAKDGKGAVQVAFMGRSAEVPFTVSGMNQSLKPDYVRDVMPV
                     LSKLGCNMGTCHGAKDGKAGFKLSLRGYDPIFDVLAFSDELASRRANVASPEHSLMLL
                     KASGSVPHEGGQLTVPGELYYETLKAWIGQGAKLDLKTPRVASIEVFPKNPVIERVGG
                     RQQMRVVARYADGYVKDVTAEAFLESGNGDVIEADTTGLMTSLRRGEAAILARFEGAY
                     AATTITVMGDRTGFAWKQPETWGKIDELVAKKWERMKIEPSGLAGDEEFLRRVHLDLT
                     GLPPKPEDVRAFLADTRETRVKREAVIEKLIGHPDFVDHWANKWADMLQVNSKFLGTE
                     GAASLRAWIKQQVTDNTPYDRMAYRIITATGSTKDNPAANYFKTVRNAEDLVETTTHL
                     FLATRFNCNKCHDHPFEKWTWDQYYETAAFFAQVDIKPDPASAGKTIGGTAVEGAKPL
                     YEIIGDKAEGEMNHLRTNAPVAPRVPYDTQLISQGPAAPKSRREQFAAWMTSPDNDYF
                     AMSYANRIWGYLTGTGVIEPLDDIRAGNPPSNPELLQYLTNEFVQSGFDVRHLMRLIT
                     KSRTYQLSLATNKWNADDTVNYSHAKARRLPAEVLFDAVFAATGSQPNIPGVPKGTRA
                     AQLADGQIQPADGFLANFGKPARESVCECERSNEVNLGPVMALMSGPTVGDAISDPNN
                     AIAKLTKEVADDRKLVEEIFVRVLNRLPTDKEIAAALASMESMDAEHKALTAEWQAKE
                     AEQKPHIEKAEADRLAAIAAAKQELEAYKVKMAPEWKKKEEARLAAIKKAEEAVKKAA
                     EAAPAQQPRWENYLDLTTLWEPLEMKVTRAGGVAKLESQPDKSLLATLLPNGQLAPGN
                     YQLQGRTALKGITAIKLEVLPDDRLPNNGPGIAPDGNFVLSEIVVSASPADAKRAKAA
                     AQAITLRNPRADFEQANFPVTESLKKGNRDRGWAVSPEGGFRHEAIFEFDKPVDFDGG
                     ALLNVQLTQFYQNGKYNLGKFRLWVTTAPVVRFGTPKIVAEAMKLPAGKRSKEQQAAL
                     AAHFLEQSRDYQTQKKALAAASKPLPQDEPLLALETRLAVTEKPIVLDPKLVQLRRDA
                     DLSKKQLADRRLTAAQDLAWALINSPAFLFNH"
     gene            complement(5671..6555)
                     /locus_tag="PLP58_16150"
     CDS             complement(5671..6555)
                     /locus_tag="PLP58_16150"
                     /inference="COORDINATES: protein motif:HMM:NF015431.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HRJ10112.1"
                     /translation="MNVHHLELFHFVAKHGGVSAAARHMPYGIQQPAISAQILQLEDA
                     LGVTLFQRRPFKLTAQGRELHEFIAPFFNGLGALEERLRGGSGGRLRIAAPEIVQGDY
                     LPLLLRQVRKRLPEFHFTLVAERIGGIEEQLRDQQVDIGLASMSGRCPDGLRMRELLR
                     LPMLLLVAEKSRVTSAEQILSQDRISQPLISLPATEPVSRLFQAELARRKLNWNPAFE
                     LSSLDLVSRYVAEDYGVGLTLAAPARRWPKGVRALPLPDFPGVVFCALWSGRLTPAGE
                     VFMEEAARLARSLSQVKM"
     gene            complement(6596..6787)
                     /locus_tag="PLP58_16155"
     CDS             complement(6596..6787)
                     /locus_tag="PLP58_16155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ10113.1"
                     /translation="MHGSALLAVIHWWCDLTKWRRYAFCLALIAADTVLAFAGKQWFA
                     GWAVGLVLLGCTFLGVGED"
     gene            complement(6842..7987)
                     /locus_tag="PLP58_16160"
     CDS             complement(6842..7987)
                     /locus_tag="PLP58_16160"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF021778.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052689 - carboxylic ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lactonase family protein"
                     /protein_id="HRJ10114.1"
                     /translation="MKPTSLSFVFAALTTTAIAGDNYLVYFGCYTNAKSGSKGIYVSK
                     FNSTTGKLTAPELAAETGSPSFLVIHPSKKHLYAVGEMSGPDKKAGGVSAFKINLPGG
                     RLDFINQVSSVTAGPCHINLDATGRMAMIANYSGGSCASYQIREDGGLGEPASHHQHT
                     GSSVNERRQKEPHAHSVNISPDNRFALVCDLGTDKVSVYAIDPGSGRMTPHEPAFAKV
                     PAGGGPRHLAFHPNGKFVFVNNELTMTETVFAWDAEKGVLTEIETVSTLPEADRGREG
                     FSTAETVVHPNGRFVYVSNRTHDTIAVFACDPSTGKLTLIQNAPAEGQIPRNFNLDPS
                     GRWMIVAHQNSNTAAVFKVDPENGKITFTGDKVNVGGAVCVRFLALD"
     gene            complement(8141..>11131)
                     /locus_tag="PLP58_16165"
     CDS             complement(8141..>11131)
                     /locus_tag="PLP58_16165"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009965120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HRJ10115.1"
                     /translation="GLPTRAADTQKCIRAGGKHNDLEDVGLDTYHHTLFEMLGNWSFG
                     DYFKKEAIAWAWELVIERWKFPPTRVYATIYQPDKSKGDPADRDQEAWEIWAEKFRSI
                     GLDPDIHIVNGNKKDNFWMMGDTGPCGPCTEIHIDLTPGTPELDEARQREGRKLVNGS
                     DASCIEIWNNVFIQYNATPEGTFEPLPAKHVDTGMGFERVCSIIQGTKNFTDFQNAKI
                     SNYDTDVFRPIFDELTKMSGKTYASTLPKANEQGHVVPVTEQEKIDVAFRVIADHIRT
                     LSFAIADGIQPGNSDRNYTLRRILRRAVKYGRTLGFDGSVSFFFKLVDVLADQFRNVF
                     PNVHEKREHIKAVLKLEEEAFNRTLDKGTEDLEGVTNAIKCIQQFLSEVPGRCSELEG
                     FIKKKGLSVCGSQEEQDKLWKLVFSITKLPVDFDAAKLAWDGVSSASSRQQQSFESAL
                     TNWQLTWTQKTWGLYDAINDWAIDGFTEQFFQQNANRMLDGIARAIVEQQREVVTSKV
                     VSAEVAFRLYDQEGFPLDLTELMARERGLTVDTAGFEELMDQQRARARAAMKKNVVSV
                     SEIETKAPTKFIGYDNLEADVKVLEVVEMKDKTAVVLDVSTCYAEMGGQIGDAGQIIL
                     GANTFPITATTKVGNTWLHFLGGDEAPPVDVTVKLVVDAPRRHAIERHHTVTHILHWA
                     LHEVVSKDATQKGSSVTPDKLTFDFNSAALTAQQLADIERLVNERIVANDPVSWSEVP
                     YVEAKANNGIMALFGEKYGDLVRVVQIGGQPHKLDGWSMELCGGTHTRHTGEIGLFRL
                     VGEGAVAAGVRRIEAIAGLEAYNAAKADSDLLKLLAGKVSAQGTADLEKKLDQILTQQ
                     KELEKALKAAQQREASGKAKELLASVEDDVSLPSGERSRVPLLVANLGDVDADYAMAV
                     NDALKGQFPGVIVLAATGGGSVALIANVSKDLQSKVQAGKIIQTIAPIVGGKGGGKPD
                     FARGGGKDVSQVDAALAEARKLVG"
BASE COUNT         2089 a         3551 c         3528 g         1963 t
ORIGIN      
        1 ctgggcagct cgcgcttctc cagatcccgc ttcagagtgg cgatggtcct gcgcgcctcc
       61 ttgagctccg cctccaactg cgccacgcgc gggtccggcg cggcgggcgt gcccagcacc
      121 caggcgagcg ccagcacgga tccgagggtg gcgagcccga tcaagacgag gagcaggttt
      181 ttcatgactg gctcgtggtt taccatccgc gcgatggaac tgccagacag aaatgcgcgg
      241 atgccgttgg agtccaagcg ttggagtcca agctttagct tgttccccaa tctcccgcgg
      301 cgaatcccac tcccgtggcg cctgaaggct tcactccaac gggcttgcca agtccgccga
      361 tttttcccgc gcccgcatgg atgatcccgc ctggatgggg tggtcaacgc aagagaccac
      421 gcaggcgcgg cgttttggcg cgttcacttg gttagaacca ccctccaacc tgccatgacg
      481 aaatcccttc ttccacttct cgccctgctg acggcgctgc ccgcctttgc caaggaggag
      541 gccgcagacc cgaacaagcc cgtctcgttt tacaaggaca tccgccccat tctccaggcg
      601 aactgcaccg gctgccacca gcccgcgaag gcgaagggcg attatgtgat gacggacttc
      661 gccaagctgc tggccggtgg cgaggagggt gatgccgtca ttcccggcaa gcccgaggcg
      721 tccaacattc tcaaggtctc cacgcccggc ccggacggca aggcggagat gccgccgaag
      781 ggagattctc tccatgccac gcagctcgcg ctcatccaaa aatggatcac cgaaggggcc
      841 aaggacgaca cacccgcctc ggcgctggcg catttcgaca tgaacaatcc gccggtctat
      901 gtgactccgc cggcggtgac ctcgctggag tattcgccgg atggcaaatc catcgcggtg
      961 gcgggttatc atgaggtctt gattcacaag gccgatggca gcggcatcga ggcgcgtctg
     1021 gtgggcctct ccgagcgtgt gcagaagctg gcctggagcc cggatggcaa aaagatcctc
     1081 gtcaccggcg gcagcccggc gcgtatgggg gagattcaaa tctgggacgt ggcggccaga
     1141 aagctggagc tttccaagtc gctcaccttt gacacgctct atggcgcaag ctggtcgccg
     1201 gatggcaagt tcatcgcggt gggctgcggc gacaacggcc tgcgggcctt cgaggcggcg
     1261 agcggcaagg aggtcgtctt catgggcggt cactcggact gggtgctcga cacggtttgg
     1321 ggcgtggatg gcaaacacct cgtctcctgc gggcgcgaca tgagcgtgaa gctcacggag
     1381 gtggagacgc agcgcttcat tgacaacatc acctccatca cgccaggcgc gctgaagggc
     1441 ggcgtgcagg cgctggcgcg gcatccgcag cgcaatgaaa tcctcatcgg cggcacggat
     1501 ggcgtgcccg ccatctaccg catggagcgc atcaccaagc gcgtcattgg cgacaacgcc
     1561 aacctcatcc gccagttccc cgcgatgaaa ggccgcatct tcggcgtggc cttcgcgccg
     1621 gatggcaagc gcatcgctgc gggcgcctcg cacgagggca cgggtgcggt gaacatctac
     1681 tccagcgagt atgacagcgc catgcccgac gccgtgaaaa agatcgtcga gacagtgaac
     1741 ggcaagggca aggaactcga tgactggatc accaaggacg tgaagctgct gagcagcacc
     1801 cccatcgcca ccggcggcgt tttcagcgtc agcttcagcc cggatggcat gaccgtggcg
     1861 gcggcaggac aagacggccg cgtgaagctc atcgaggctg cgacgggcaa gatcgccaag
     1921 gaattcctca gcgtgccgct cgtggaggac aaggcgctgg ccgcgagtga cgtgatcgcc
     1981 gacgacagcg tgcgcgccac ggcgaagaag gacgacaagg tcgagacgct ggtgccggga
     2041 ctgaccatcg cctccattga ggtcgagccg aaaagcatcg ccatcggcaa gccaaacgag
     2101 tatgcacagc tcctcgtgac ggccacaatg aacgacggca gcaaggccga catcacacgc
     2161 atggccaagc tcagctccag cgcggacgcc gccagcgtca acgcgcgcgg cctggtgcgc
     2221 ccggccaagg atggcaaagg cgcggtgcag gtcgctttca tgggccgcag cgccgaggtg
     2281 cccttcaccg tcagcggcat gaaccaaagc ctcaagcctg actacgtccg cgatgtgatg
     2341 cccgtgctct cgaagctggg ctgcaacatg ggcacctgcc acggcgcgaa ggacggcaag
     2401 gccggcttca agctcagcct gcgcggttac gaccccatct tcgacgtgct cgccttcagc
     2461 gacgaactcg ccagccgccg cgccaacgtg gcctcgccgg agcactcgct catgcttttg
     2521 aaggccagcg gctccgtgcc ccatgagggc ggccagctca ccgtgccggg cgagctttat
     2581 tatgagacgc tcaaggcgtg gatcggccag ggcgcgaagc tggacctcaa aaccccgcgc
     2641 gtcgccagca tcgaggtctt cccgaaaaac ccggtcatcg agcgtgtcgg cggccgccag
     2701 caaatgcgcg tggtggcgcg gtatgcggac ggttatgtga aggatgtcac cgccgaggcc
     2761 ttcctggaaa gcggcaatgg cgatgtgatc gaagccgaca ccaccggcct catgaccagt
     2821 ctccgccgcg gcgaggccgc catcctcgcg cgtttcgagg gcgcctatgc cgccaccacc
     2881 atcaccgtca tgggcgaccg cactggcttc gcctggaagc agccggagac ctgggggaag
     2941 attgacgagt tggtcgccaa aaaatgggag cgcatgaaga tcgagccgag cggtctggcg
     3001 ggcgatgagg agttcctccg ccgcgtccac ctcgacctca ccggtctgcc gccgaagccc
     3061 gaggacgtgc gcgcctttct cgccgacacg cgcgagaccc gagtgaagcg cgaggccgtc
     3121 attgaaaagc tcatcggcca tcccgacttc gtggaccact gggcgaacaa gtgggccgac
     3181 atgctgcagg tgaactcgaa gtttcttggc accgagggcg cggccagcct gcgcgcgtgg
     3241 atcaagcagc aggtcaccga caacacgccc tacgaccgca tggcctaccg catcatcacc
     3301 gccaccggct ccaccaagga caaccccgcc gccaattact tcaagaccgt gcgcaatgcc
     3361 gaggacctcg tggaaaccac cacccacctt ttcctcgcca cccgcttcaa ctgcaacaag
     3421 tgccacgacc accccttcga gaaatggaca tgggaccaat actatgagac cgccgccttc
     3481 ttcgcccagg tggacatcaa gcccgatccc gccagcgccg gcaagaccat cggcggcacc
     3541 gccgtggagg gagccaagcc gctttatgaa atcattggcg acaaggccga gggcgagatg
     3601 aaccacctgc gcaccaacgc ccccgtggct ccgcgcgtgc cttacgacac ccagctcatc
     3661 agccagggcc ccgccgcgcc gaagagccgg cgcgagcagt tcgcggcctg gatgaccagc
     3721 ccggacaacg actacttcgc catgagctac gccaaccgca tctggggcta cctcaccggc
     3781 accggggtca tcgagccgct ggatgacatc cgcgccggca atccgccgag caatcccgaa
     3841 ctgctgcaat acctcaccaa cgagttcgtg cagagcggct tcgacgtgcg ccacctcatg
     3901 cgcctcatca ccaagagccg cacttaccag ctcagcctcg ccaccaacaa atggaacgcc
     3961 gacgacacgg tgaactacag ccacgccaag gcgcgccgcc tgcccgccga agtgctcttt
     4021 gacgccgtct tcgccgccac cggatcgcag ccgaacatcc ccggcgtgcc caaaggcacc
     4081 cgcgccgccc agcttgccga cggccagatc cagccagccg acggcttcct ggccaacttc
     4141 ggcaaacccg cccgcgagtc cgtctgcgaa tgcgagcgca gcaacgaggt gaacctcggc
     4201 cccgtcatgg ccctcatgtc cggccccacg gtcggcgatg ccatcagcga cccgaacaac
     4261 gccatcgcca agctcaccaa ggaagtggcc gacgaccgaa agctcgtgga ggaaatcttt
     4321 gtccgcgtgc tcaaccgcct gcccacggac aaggagatcg ccgccgcgct cgccagcatg
     4381 gagagcatgg acgccgagca caaagcgctc accgccgaat ggcaggccaa ggaggccgag
     4441 cagaagccgc acatcgagaa ggctgaagcc gaccgtctgg ccgccattgc cgccgcgaag
     4501 caggaactcg aggcctacaa agtgaagatg gctcctgagt ggaaaaagaa ggaagaggcc
     4561 agactcgccg ccatcaagaa agccgaggaa gccgtgaaaa aagctgccga ggctgccccc
     4621 gcgcagcaac cgcgctggga aaactacctt gacctcacca cactctggga gccgctggaa
     4681 atgaaagtca cccgcgccgg aggagtcgcc aagctggagt cgcagcccga caagagcctg
     4741 ctcgccacac ttctgccgaa cggccagctc gctcctggca actaccagct ccagggccgc
     4801 acggccttga aaggcatcac cgccatcaag ctcgaagtcc ttcctgacga ccgcctgccc
     4861 aacaacggcc caggcatcgc cccggacggc aattttgtcc tcagcgagat cgtcgtcagc
     4921 gccagccccg ccgatgccaa acgcgccaag gcagccgcgc aagccatcac cttgcgcaac
     4981 ccgcgcgccg acttcgagca ggcgaacttc cccgtcaccg agtccctgaa aaaaggcaac
     5041 cgcgaccgcg gctgggcggt cagccccgag ggaggcttcc ggcacgaggc catcttcgag
     5101 ttcgacaagc ccgtggactt cgatggcggc gctttgctca atgtgcagct cacgcagttc
     5161 tatcagaacg gcaaatacaa cctcggcaaa ttccgcctct gggtcaccac cgcgcccgtc
     5221 gtccgcttcg gcacgccgaa gatcgtcgcc gaagccatga agctgcccgc tggcaaacgc
     5281 agcaaggagc agcaagccgc gctcgccgcc catttcctgg agcagtcccg cgactaccag
     5341 acgcaaaaga aagccctcgc cgccgccagc aagcccctgc cgcaggacga gcccctgctc
     5401 gccctcgaaa ccaggctcgc cgtgaccgag aagcccatcg tgctcgaccc caaactcgtc
     5461 cagctccgcc gcgacgccga cctcagcaag aagcagctcg ccgaccgccg cctcaccgcc
     5521 gcccaggacc tcgcctgggc gctcatcaac tccccggcct tcctgttcaa tcattgaggc
     5581 cgcattgctg gtgctggtgt ccaggcttca gccgctgtga attccaagcg gcgaagccgc
     5641 cgcattttca gaccggctga attgctcccc tcacattttc acctgcgaca atgagcgcgc
     5701 caggcgggcg gcctcctcca tgaagacctc gccggccgga gtgagccgcc ctgaccagag
     5761 cgcgcagaag accacgccgg gaaaatcagg cagcggcagc gcgcgcacgc ccttcggcca
     5821 gcgccgcgcg ggcgcggcga gcgtgagtcc cacgccgtaa tcctccgcca catagcggct
     5881 cacgaggtcc aggctgctca gttcgaaggc ggggttccag ttcagttttc tccgcgccag
     5941 ctccgcctgg aacaagcggc tcaccggctc ggtcgcgggc aggctgatga gcggttgcga
     6001 gatgcggtcc tgggagagaa tctgctccgc cgaggtgacg cgggattttt ccgccaccag
     6061 cagcagcatg ggcaggcgca aaagctcgcg catcctgagg ccgtccgggc agcgcccgct
     6121 catcgaggcc aggccgatgt ccacctgctg gtcccgaagc tgctcctcga tgccgccgat
     6181 gcgctcggcg accagggtga agtggaactc cggcagcctc ttgcgcacct gccgcagcag
     6241 cagcggcagg tagtcgccct gcacgatctc gggcgcggcg atgcgcagcc tgccgccgga
     6301 gccgccgcgc agccgctcct ccagcgcgcc gaggccgttg aagaagggcg cgatgaactc
     6361 gtgcaactcc cgcccctgcg cggtcagctt gaaggggcgg cgctgaaaca gggtcacgcc
     6421 cagcgcgtcc tccaactgca aaatctgcgc gctgatcgcc ggctgctgga tgccgtaggg
     6481 catgtgccgc gccgccgcgc tcacaccgcc atgcttcgcc acaaaatgga acagctcgag
     6541 atgatggacg ttcatgcgcg gatcatgccc ggcctttggc gaaatgccac gcggatcaat
     6601 cctcccctac tcccagaaat gtgcagccca aaagcaccag tcccaccgcc cagccggcaa
     6661 accactgctt gcccgcgaac gcgagcaccg tgtctgccgc gatcagagcg agacagaatg
     6721 cgtaacgccg ccacttcgtg agatcacacc accagtggat gacagccagg agggcggagc
     6781 cgtgcatgaa ttgatcgcag tgggggcagt gtcgagggaa ccaaacgcaa atccccgccg
     6841 ctcagtccag cgccagaaaa cgcacacaca ccgcgccgcc gacattcacc ttgtcaccgg
     6901 tgaaggtgat tttgccgttt tccggatcga ctttgaagac ggcggcggtg ttgctgttct
     6961 ggtgggccac gatcatccac cggcccgagg gatcgaggtt gaaattgcgc gggatctggc
     7021 cttcggcggg cgcgttttgg atgagggtga gtttgcctgt ggagggatcg cacgcgaaga
     7081 cggcgatggt gtcgtgagtg cggttcgaga catagacgaa cctgccgttc ggatgcacga
     7141 cggtctcggc ggtgctgaaa ccctccctcc cgcggtcggc ctcaggcagg gtggagacag
     7201 tttcgatctc ggtgagcacg cctttttccg cgtcccaggc gaagacggtc tcggtcatcg
     7261 tcagctcgtt gttcacgaag acgaacttgc cgttcgggtg gaaggccagg tggcgcgggc
     7321 cgcccccggc gggcaccttg gcaaaggcgg gctcgtgcgg cgtcatacgc ccgctgccgg
     7381 ggtcaatggc ataaacgctg accttgtctg tgccgagatc gcacacgagg gcgaagcggt
     7441 tgtccgggct gatgttgacc gagtgcgcgt gcggctcctt ctggcggcgc tcattgacgc
     7501 tggagccggt gtgctggtgg tggctggctg gctcaccgag gccgccgtcc tcacggattt
     7561 gataggaggc gcaggagccg ccgctgtagt tggcgatcat ggccatgcgc ccggtggcgt
     7621 cgaggttgat gtggcagggg ccggcggtga cggagctgac ctggttgatg aagtcgagcc
     7681 tgccgccggg caggttgatc ttgaaggcgc tcacaccgcc cgccttcttg tcaggtccgc
     7741 tcatctcgcc aacggcatag agatgttttt tcgagggatg gatgacgaga aagctggggc
     7801 tgcccgtctc ggcggcgagt tcgggcgcgg tgagcttgcc ggtggtggag ttgaatttgg
     7861 aaacgtaaat gcctttgctg cccgacttgg cgttcgtgta gcagccaaaa tagacgaggt
     7921 agttgtcacc ggcgatggcg gtggtggtga gggcggcgaa gacgaaggaa agggaggtcg
     7981 gtttcatggc aggtgcggac aagctaacgc tccaatgcca gtggaacttc acagacatca
     8041 cacgcgccaa caactcctgc attctgacga atgcgcctgc ggtgccgcgc aggcgcgctt
     8101 cccaaagcgc ggaggttggc cctcataggt tgtccagtct tcagccgacc agcttgcgcg
     8161 cttcggcgag ggcggcatcc acttgggaca cgtccttgcc gccgccgcgg gcgaaatccg
     8221 gcttgccgcc gcctttgccg ccgacgatgg gggcgatggt ttggatgatc ttgccggcct
     8281 ggaccttgct ttgcaggtct ttggagacgt tggcgatcaa ggccacgctg ccaccgccgg
     8341 ttgccgcgag gacgatgacg ccggggaact ggcctttcag agcgtcgttc acggccatgg
     8401 cgtagtcggc gtccacgtcg ccgagatttg cgaccagtag aggaacgcgt gaacgttccc
     8461 cggagggaag actcacgtca tcctctacgg acgcgagcag ctctttcgcc ttgccgctgg
     8521 cttcgcgctg ctgggcggct ttgagggctt tttcgagctc tttttgctgc gtgaggattt
     8581 ggtcgagctt cttctcgagg tcggcggtgc cttgggcgct gactttgccg gcgaggagct
     8641 tcaggaggtc agaatcggct ttggcggcgt tgtaggcttc gaggccggcg atggcttcga
     8701 tgcggcggac gccggcggcg acggcgcctt cgccgacgag gcggaagagg ccgatttcgc
     8761 cagtgtggcg ggtgtgggtg ccaccgcaga gttccatgct ccaaccatcg agcttgtgcg
     8821 gctggccgcc gatctgcacg acgcggacga ggtcgccgta tttttcgcca aagagggcca
     8881 tgatgccgtt gttggccttg gcctcgacgt agggcacttc gctccacgaa acggggtcat
     8941 tggcgacgat gcgctcgttc acgaggcgtt cgatgtcggc gagctgctgc gcggtgagcg
     9001 cggcgctgtt gaagtcgaag gtgagtttgt ccggcgtgac ggatgagcct ttctgcgtgg
     9061 cgtccttgct gacgacctcg tgcaacgccc agtgcaaaat gtgcgtgacg gtgtggtgtc
     9121 gctcgatggc gtgacgacgc ggcgcatcga cgacgagctt cacagtgaca tcgacaggcg
     9181 gggcttcgtc gccgccgagg aagtggagcc aggtgttgcc gactttcgtc gtggcggtga
     9241 tggggaaggt gttcgcgccg aggatgatct ggcccgcgtc accgatctgg ccgcccattt
     9301 cggcgtagca ggtggagaca tcgagcacga cggcggtttt gtccttcatc tcgacgactt
     9361 cgagcacctt cacatcggct tcgaggttgt cgtaaccgat gaacttggtc ggggccttcg
     9421 tttcgatctc ggagacggag acgacgtttt tcttcatcgc ggcacgggca cgggcgcgct
     9481 gctgatccat gagttcttcg aagccggcgg tgtccacggt gagaccgcgt tcgcgggcca
     9541 tgagttcggt gaggtcgagc gggaagcctt cttggtcgta gaggcggaat gccacttcgg
     9601 ctgaaacgac tttgcttgta acgacctcgc gttgttgctc cacaatagct ctcgctattc
     9661 catcaagcat ccggttggcg ttctgttgga agaattgctc agtgaagcca tcaatcgccc
     9721 aatcgttaat cgcatcgtaa agcccccacg ttttttgcgt ccaggtcagt tgccaatttg
     9781 ttagagcact ttcaaagctc tgctgttgcc gtgagcttgc actcgaaaca ccatcccagg
     9841 ccaatttggc agcatcaaaa tcgacaggca gtttagttat cgaaaaaaca agcttccaaa
     9901 gcttgtcttg ttcttcttgg gagccgcaga cagaaagccc ctttttcttg atgaagcctt
     9961 ccagctcaga gcaacgcccc ggaacttcac tgaggaactg ttggatacat ttgatagcgt
    10021 tagtgacgcc ctccaagtct tccgtgcctt tatcaagggt ccggttgaat gcttcttcct
    10081 caagcttcaa caccgctttg atgtgctctc tcttctcatg aacattcgga aacacatttc
    10141 ggaattggtc tgctagcaca tctaccagtt tgaagaagaa tgacacggat ccatcaaaac
    10201 ctagcgtgcg gccgtatttc acggcgcggc ggaggatgcg gcggagggtg tagttgcggt
    10261 cgctgttgcc gggctggatg ccgtcggcga tggcgaagct gagcgtgcgg atgtggtcgg
    10321 cgatgacgcg gaaggcgacg tcgatctttt cctgctcggt gacggggacg acgtggcctt
    10381 gctcgttggc cttcggcagc gtgctggcat acgttttgcc agacatcttc gtcagctcgt
    10441 caaagatggg acggaagacg tcggtgtcgt agttgctgat cttcgcgttt tggaagtcgg
    10501 tgaagttttt ggtgccctgg atgatggagc agacgcgctc gaagcccatg ccggtatcga
    10561 cgtgcttcgc ggggagcggc tcgaaggtgc cttccggtgt cgcgttgtat tggatgaaga
    10621 cgttgttcca gatctcgatg cagctggcgt cgctgccatt cacgagcttg cggccttcgc
    10681 gctggcgggc ttcatcgagc tcgggcgtgc cgggggtgag gtcgatgtgg atctcggtgc
    10741 aggggccgca ggggccggtg tcgcccatca tccagaagtt gtccttcttg ttgccgttga
    10801 cgatgtggat gtcgggatcg aggccgatgc tgcggaattt ttccgcccag atttcccatg
    10861 cctcctggtc gcggtcggcg ggatcgccct tggatttgtc gggctgataa atggtggcgt
    10921 aaacgcgggt gggcgggaac ttccagcgct cgatgacgag ctcccaggcc caggcgatgg
    10981 cctctttctt gaagtagtcg ccgaaggacc agttccccag catctcgaag agcgtgtggt
    11041 ggtaggtgtc gaggcccacg tcttcgaggt cgttgtgctt gccgccggcg cggatgcact
    11101 tctgcgtgtc cgcggcgcgg gtgggcaggc c
//
LOCUS       DAOMYJ010000177        11026 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Prosthecobacter sp. isolate AMR_MDS_5629
            NODE_1975_length_11026_cov_21.862182, whole genome shotgun
            sequence.
ACCESSION   DAOMYJ010000177 DAOMYJ010000000
VERSION     DAOMYJ010000177.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867850
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prosthecobacter sp. (wastewater metagenome)
  ORGANISM  Prosthecobacter sp.
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Verrucomicrobiaceae; Prosthecobacter.
REFERENCE   1  (bases 1 to 11026)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11026)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.30
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:34:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,742
            CDSs (total)                      :: 4,684
            Genes (coding)                    :: 4,676
            CDSs (with protein)               :: 4,676
            Genes (RNA)                       :: 58
            tRNAs                             :: 55
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11026
                     /organism="Prosthecobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1975_length_11026_cov_21.862182"
                     /isolate="AMR_MDS_5629"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1965333"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..422)
                     /locus_tag="PLP58_16170"
     CDS             complement(<1..422)
                     /locus_tag="PLP58_16170"
                     /inference="COORDINATES: protein motif:HMM:NF019011.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M81 family metallopeptidase"
                     /protein_id="HRJ10116.1"
                     /translation="MKLPLLSLLALTVCAHAAPPPRIAVMQLRHETVTFLPNETTRED
                     FIYPGSPAKGEALLKTQPTGDMGGFVKVAREYGAVLAGIESPGMPETGIGSGWVTRDA
                     YEHFTGRMIEELKAQGPFDGVYLALHGAMAVRGIARPEA"
     gene            544..3471
                     /locus_tag="PLP58_16175"
     CDS             544..3471
                     /locus_tag="PLP58_16175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009958263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M28 family peptidase"
                     /protein_id="HRJ10117.1"
                     /translation="MIRTATFFLLAALSLHAQSEPDFLTGIRQLTFEGRRAGEGYFSA
                     DGSKLVFQSEREPGNPFYQIYLMDLETGDQERVSPGMGKTTCAWIHPDGKRVLFASTH
                     SDPKSKELQDAEYKERESSRVRKYSWDYDEHYDIWEYHLEDKSLKNLTSTRGYDAEGA
                     WSPDGKRMVFASNRAAYEGTLSKEDAARLENDKQYFMDIYVADADGANVKRLTDVPGY
                     DGGPFFSADGTRICWRRFTPKGDVAEVWTMNTDGTDQRQLTKLGAMSWAPYFHPSGEY
                     LIFTTNLNGFANFELYVVDAAGKKDPVRVTHTDGFDGLPVFSPDGKKLAWTSGRTSNG
                     QSQIFLADWDHDHARAALGIGASKPEAQPANAPDLTLTAAEIRPDDLRQHVTYLASDE
                     LDGRLTGTEGERLATQYVADVFHGIGLLPYGDEEWFEPFDFTAGVALGDGNSLTLTGG
                     EKRDFTADKDWRPLSFSQTGEVQAAPVVFAGYGVETPDEATGADGKALEPYSSYAHLD
                     VKDKWVLVLRYLPEGLSKERRGDLTRYASLRHKAMVARQKGARGLIIASGPNSKVVQQ
                     LVPMAFDASLASSGISAISVTDALADVLLKPAGKTLKELQDKLDTGDLMGGIECGKLT
                     LAARIDIQQERKKGRNVLGVLPARDKPDPHVAPLIVCAHIDHLGSKGGSNSRARGDDA
                     NRVHHGADDNASGIAGMIEIAQWLADQKRQGKVKLTRDVIFAAWSGEELGLLGSNHFA
                     ESLAKMFTGDANGKLTNMFAACLNMDMIGRFQKTLVLQGVGSSTWWPKEIEKRNAVIG
                     LPITTQNDAHLPTDSTTFYLRGIPTLNAFTGSHEDYHLPSDTADKLDNDKAAQITRLI
                     GLIARAVATADTNPDFVAMEAPKSQGTRGGLRAYLGTIPDYAQGDVKGVKLSGVSPIG
                     PAGKAGIKGGDIIIKLAGKEVLNIYDYTSLMGELKIGTETNITVLREGKEVELKITPS
                     SRD"
     gene            3583..3780
                     /locus_tag="PLP58_16180"
     CDS             3583..3780
                     /locus_tag="PLP58_16180"
                     /inference="COORDINATES: protein motif:HMM:NF040165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ10118.1"
                     /translation="MASEITPEYLATLRGMTGAQKLRTAFQLYWGARRLKAARLRQQH
                     PDWTEEQIQQRVKEIFMHAVT"
     gene            3767..4333
                     /locus_tag="PLP58_16185"
     CDS             3767..4333
                     /locus_tag="PLP58_16185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ10119.1"
                     /translation="MQSPEPYLIFTRKLQELGLRHMVSGSVAAIYYGEPRLTNDVDII
                     VFLRREDARRIITAFPPDDFYCPPLEVIEAERVRDQRGHFNLIHHETGFKADIYLTGR
                     DELHAWGFAHAVSADLDGDTVVFAPPEYVIVRKLQFFREGGSQKHLRDIHRMLLGLGD
                     DWPRGTLMEMLRTHRLEPEWSLVKSWDE"
     gene            complement(4638..5858)
                     /locus_tag="PLP58_16190"
     CDS             complement(4638..5858)
                     /locus_tag="PLP58_16190"
                     /inference="COORDINATES: protein motif:HMM:NF037031.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M14-type cytosolic carboxypeptidase"
                     /protein_id="HRJ10120.1"
                     /translation="MNLPRLLFLPVLCLLIAARVHSAGLTLHSDFPGGSAEVHALDAD
                     AGLIEISPVSREGRGWPCWWYFRVDGARPGQALTVRVKPAGRPFRAQERLAAAWSLPA
                     RAAISSDDQTWRHTEPGTVTKTDATYAITAPAATFWLAWGPPFLPAHSEALLEQTASR
                     VPGAERFVLAKTREGREVPGMRLGKKDAPQAVWVHARQHAWEAGGSWVGRGFLEWVAG
                     DTPEAAALRASTEIFFIPIMDVDNVALGAGGKEAVPRDHNRDWDTRPVYPEVAAAQQR
                     VEALIQVGRLRCYFDLHNPGSGDKQPFFFGPMDYEQMTGPRRASYDRFLSLCVQHMRE
                     PLPILPRYRFATYVKTQEERDRMSGNWVRDRTPARAVAMTLETAWNTPHSTQDGYLHV
                     GQGLAKAMAAWLRE"
     gene            complement(6024..7202)
                     /locus_tag="PLP58_16195"
     CDS             complement(6024..7202)
                     /locus_tag="PLP58_16195"
                     /inference="COORDINATES: protein motif:HMM:NF024771.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enolase C-terminal domain-like protein"
                     /protein_id="HRJ10121.1"
                     /translation="MKITAIETLVCHARMRNWVFVKVVTDQPGLIGWGEATLEWHTRS
                     IVGAIEDMSHLLIGEDPTRVEYLWQMMYRQHFWHGHGIVRATAIAGIDLALWDIVGKV
                     ANMPLSKVFGGPVRDWVRTYCHLGGGKMEDFYQTPADNAKRFAELAQQAVADGYTAFK
                     SMAVPSTMPIEGMKPVRAAAAAVAAMRDAVGPDIDIMVDCHARPSPAMGLKFGKALDD
                     FGLYFFEEPCWPEAVDGLAKINAALTTPVASGERVTNLEAFKEMFDKRAVEICQLDIT
                     HCGGLSAAKRIAALAEAHRIALAPHNPQGPVSTAASLEFGFSQPSYIICETVHNDVPW
                     RQDVVEEGFIIEKQGRIVRPNTKPGLGITINEAEVRKHPFQQEIVLREFSPDGAVTDW
                     "
     gene            complement(7199..7603)
                     /locus_tag="PLP58_16200"
     CDS             complement(7199..7603)
                     /locus_tag="PLP58_16200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ10122.1"
                     /translation="MNKANIVSLWLEACPSFRTAFVESCTENGSELLYLHAAAFARHL
                     LELQRLEKTNEFGAVAIFIEGLHTDDDPATREFATIGILEGIQNVWGHSEVSPNEFLP
                     YLLPASAAAWRKLDQFWSGDIPFIPDTHHLSS"
     gene            complement(7609..9030)
                     /locus_tag="PLP58_16205"
     CDS             complement(7609..9030)
                     /locus_tag="PLP58_16205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ10123.1"
                     /translation="MPALSRTEDPTPQSRCRFALKWCDITPPADIYHRMWGAAKHERA
                     TGVHRPLRATVTVFAPMEGSERQIVLALDHCVMGATEHAKLVAQAAEIGGVAKEEILV
                     VFSHTHGAGLMGLDRASLPGGELIPPYLQSLGEKAGALIRDAIASLKPAGIVYGRGRC
                     SLATHRDFWDGKQFVCGHNPGVPADDTVIVARVTDDAGQMLASIVNYACHPTTLAWEN
                     TLISPDYIGAMREPVERDTDAPCLFLQGASGELGPREGFVGDTAVADKNGRELGYAAL
                     SALTALPAAGTTFVYQGPIVSGATLGSWKHQKIDLAEKAVWKLQRWHEPLKYRPGQPT
                     IEQTQAELDQFKAAEAAARAAGDETRAADCRAMAERKTRLLHRLHQLPPGDIYPLQVV
                     LWHIGDAVWLGVQGESYNLLQTELRRRFPNKIIIVSTIAADWGASYLPPRELYDTGIY
                     QESIAVVAAGSLEQLIEVIAKRI"
     gene            9103..10629
                     /locus_tag="PLP58_16210"
     CDS             9103..10629
                     /locus_tag="PLP58_16210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ10124.1"
                     /translation="MRPFLLLFAAINTFAADPIAIGSRRELFVDDLLIEKLSVKAEQR
                     LHHSQAKEIAIKHDAPWEGSGTGYHSVFKDGDKYRMYYKAWQLTVTPPGKVNTGEHPL
                     FTCYAESDDGIHWRKPELGLFEFKGSKANNIVLAPGKMGAANPDPGHIAIFKDDNPAA
                     KPEARYKAIVRSGSPKGLLAFQSPDGLKWSPMQETPVITDGAFDSQNLAFWDAHSGVY
                     RAYWRYFTGGTTDEKNWKPPGFRAIRTATSKDFLHWENQQNLSYIDSPDEALYTNQVK
                     PYARAPQLLFGFPTRYIERGWSESMRALPEREHREWRSKANDRYGMAVTEGLFMASRD
                     GVKFKRWNDAFLPPGIERPGTWNYGQQYIAWHLVETKSALEGAPDELSLYGGENYWTG
                     TSSELRRYTLRLDGFVSIHAPMSGGELITKPFTFTGKTLTMNFATSAAGGIRVEIQDA
                     NGKAIPGFTLDDSEEHFGDSIQRTVFWKSGNDVSALAGKAVRLRFVLKDADLYAIKFE
                     "
     gene            10766..>11026
                     /locus_tag="PLP58_16215"
     CDS             10766..>11026
                     /locus_tag="PLP58_16215"
                     /inference="COORDINATES: protein motif:HMM:NF015916.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008092 - cytoskeletal protein binding
                     [Evidence IEA];
                     GO_function: GO:0030674 - protein-macromolecule adaptor
                     activity [Evidence IEA];
                     GO_function: GO:0042802 - identical protein binding
                     [Evidence IEA];
                     GO_function: GO:0043130 - ubiquitin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SHD1 domain-containing protein"
                     /protein_id="HRJ10125.1"
                     /translation="MMPHFRRWVVTSALFCAGVFLLNAQQPAWRSWKSTDGRVVEAVF
                     LGVEAGGVKIKTRAGAEFVVPLERLSAEDQAWVAQQPAAAPPG"
BASE COUNT         2104 a         3578 c         3410 g         1934 t
ORIGIN      
        1 gcctccggcc gcgcgatgcc gcgcaccgcc atcgccccgt gcagcgcgag atacacgccg
       61 tcgaacggac cctgcgcctt cagctcctcg atcatgcgcc cggtgaagtg ctcatacgcg
      121 tcccgcgtca cccagcccga gccgatgccc gtctccggca tccccggcga ctcgatgccc
      181 gccagcaccg cgccgtattc gcgcgccact ttgacaaagc cgcccatgtc ccccgtcggc
      241 tgcgttttga gcagcgcctc gcccttcgcc ggcgagccgg gataaatgaa atcctcccgc
      301 gtggtctcgt tcggcagaaa cgtcaccgtc tcatgccgga gctgcatcac cgcgatgcgc
      361 ggcggcgggg cggcatgggc gcagaccgtg agggcgagga gggagagcag cggcagtttc
      421 atgggaaggt cgtcttacgg tttcaaaagc cgcaaaactg caagccgcgt cccgcaggac
      481 cgcccctttc ccccgcacaa gtgactgcat ttcccgttcc gccgcgtatg aatgccaccc
      541 gccatgattc gcaccgccac gtttttcctc ctcgccgcac tgtccctgca cgcgcagtcg
      601 gagccggatt tcctcaccgg catccgccag ctcacctttg agggccgccg cgcgggcgag
      661 ggctacttca gcgcggatgg ctcgaagctg gtcttccagt ccgagcgcga gccgggcaat
      721 ccgttttatc agatctacct catggacctg gagaccggcg accaggagcg cgtctccccc
      781 ggcatgggca agaccacctg cgcgtggatc cacccggacg gcaagcgcgt cctgtttgcc
      841 agcacgcaca gcgacccgaa gtcgaaggaa ctccaggacg ccgaatacaa ggagcgcgag
      901 agcagccgcg tgcgcaaata ctcctgggac tacgacgagc actacgacat ctgggaatac
      961 catctcgaag acaaatccct caaaaacctc acctccacac gcggctacga tgccgaaggc
     1021 gcgtggtcgc cggatggcaa acgcatggtc ttcgccagca accgcgccgc gtatgaaggc
     1081 acgctctcga aggaagacgc cgcccgcctg gaaaacgaca agcaatactt catggacatc
     1141 tatgtggccg atgcggacgg cgcgaatgtg aagcgcctca ccgacgtgcc cggctacgac
     1201 ggcggcccct ttttcagcgc cgatggcacc cgcatctgct ggcggcgctt cacgccgaag
     1261 ggagacgtgg ccgaggtctg gaccatgaac accgacggca ctgaccagcg ccagctcacc
     1321 aagctcggcg ccatgagctg ggcgccctac ttccacccca gcggcgagta tttgatcttc
     1381 accacgaacc tcaacggctt cgccaatttc gagctctatg tcgtggatgc cgccggcaaa
     1441 aaagaccccg tgcgcgtcac ccacaccgac ggctttgacg gcctgcccgt cttctcaccc
     1501 gatggcaaaa agctcgcctg gaccagcggc cgcaccagca acggccagag ccagatcttc
     1561 ctcgccgact gggatcatga ccacgcccgc gccgcgcttg gcatcggtgc atcgaagccc
     1621 gaagcccagc ctgcaaacgc tcccgatctc accctcaccg ccgccgagat ccgccctgac
     1681 gacctccgcc agcacgtcac ctacctcgcc agcgacgagc tggacggccg cctcaccggc
     1741 accgagggcg aaaggctggc cacccagtat gtcgccgacg ttttccacgg catcggcctt
     1801 ctgccatacg gggacgagga atggtttgag cccttcgatt tcaccgccgg cgtggccctc
     1861 ggcgatggca acagcctcac tctcaccggc ggcgagaagc gcgacttcac cgccgacaaa
     1921 gactggcgtc cgctttcctt ctcccagacc ggcgaggtgc aggccgcgcc cgtcgtcttc
     1981 gccggttacg gcgtcgagac accggatgaa gccaccggtg ccgatggcaa agcgctcgag
     2041 ccttacagca gctacgccca cctcgatgtg aaggacaaat gggtgctcgt cctgcgctac
     2101 ctgcccgagg gcctcagcaa ggaacggcgc ggcgatctca cccgctacgc cagcctgcgg
     2161 cacaaggcca tggtcgcccg gcaaaagggc gcgcgcggcc tcatcatcgc cagcggacct
     2221 aacagcaaag tcgtccagca gctcgtgccc atggcctttg acgcctcgct cgcctcctcc
     2281 ggcatctccg ccatcagcgt caccgacgcg ctggccgacg tgctgttaaa acccgccggc
     2341 aagaccctca aggaactcca ggacaagctc gacaccggcg acctcatggg cggcatcgaa
     2401 tgcggcaagc tcaccctcgc cgcgcgcata gacatccagc aggagcgcaa aaaaggccgc
     2461 aacgtcctcg gcgtcctgcc cgcgcgcgac aagcccgacc cgcatgtggc acccctcatc
     2521 gtctgcgccc acattgacca cctcggcagc aaaggcggct ccaactcccg cgcccgcggc
     2581 gacgacgcca accgcgtcca ccacggcgcc gacgacaacg ccagcggcat cgccggcatg
     2641 atcgagatcg cccaatggct cgccgatcaa aaacgccagg gcaaagtcaa gctcacccgc
     2701 gatgtcatct tcgccgcctg gtccggcgag gagctcggcc tgctcggcag caaccacttc
     2761 gccgaaagcc tcgccaaaat gttcactggc gacgccaacg gcaaactcac caacatgttc
     2821 gccgcctgcc tgaacatgga catgatcggc cgcttccaga aaaccctcgt gctccagggc
     2881 gtcggctcca gcacctggtg gccgaaggag atcgaaaagc gcaacgccgt catcggcctg
     2941 cccatcacca cgcaaaacga cgcccacctg cccaccgaca gcaccacgtt ttatctgcgc
     3001 ggcatcccca cgctgaacgc cttcaccggc agccatgagg actaccacct gcccagcgac
     3061 accgccgaca agctcgacaa cgacaaagcc gcgcagatca cccgcctcat cggcctcatc
     3121 gcccgcgccg tcgccaccgc cgacacgaac cccgactttg tcgccatgga ggccccgaaa
     3181 agccagggca cccgcggcgg cctgcgcgcc tacctcggca ccatccccga ctacgcccag
     3241 ggcgatgtca aaggcgtcaa gctcagcggc gtctccccca tcggccccgc cggcaaagcc
     3301 ggcatcaaag gcggtgacat catcatcaag ctcgccggca aagaagtgct caacatctac
     3361 gactacacct ccctcatggg cgagctgaag atcggcacgg aaacaaacat caccgtcctg
     3421 cgcgaaggca aggaagtgga gctgaaaatc acgccttcct cgcgggattg agggggtttc
     3481 tgcagccggt gtcattgacc ccggaacgcg cccacattcg cgggtcaatg ccccgcgcca
     3541 caacacttgc ggcatcagct cccagcgcct acgctcgcgc tcatggcttc ggaaatcacc
     3601 ccggaatacc tcgccacgct tcgcggcatg acgggcgcgc aaaagctgcg cacggcgttc
     3661 cagctttact ggggcgccag gaggctcaag gccgcgcggc tcaggcagca gcaccctgac
     3721 tggaccgagg agcagatcca gcagcgcgtg aaggagattt tcatgcatgc agtcacctga
     3781 accctatctc atcttcaccc ggaagctcca ggagctgggc ctgcgccaca tggtgagcgg
     3841 cagcgtggcc gccatctatt acggcgagcc gcggctgacc aatgatgtgg acatcatcgt
     3901 ctttctgcgg cgggaggatg cgcgccgcat catcaccgcc tttccccccg atgactttta
     3961 ctgcccgccg ctcgaggtca tcgaagcgga gcgggtccgt gaccagcgcg gccactttaa
     4021 tctcatccac catgaaaccg gcttcaaagc cgacatctac ctcactggac gcgatgaact
     4081 gcacgcctgg ggctttgccc atgcagtgtc cgccgacctg gatggcgaca ctgtcgtctt
     4141 cgccccgccg gaatatgtga tcgtgcgcaa gctccaattc ttccgcgagg gcggctcaca
     4201 gaagcatctg cgcgacatcc accggatgct gctcggcctg ggcgatgact ggccgcgtgg
     4261 caccctgatg gaaatgctcc gcacccaccg cctggaaccg gaatggtccc tggtgaaaag
     4321 ctgggacgaa tgagcgggcg gaggaagcct ccggcggcat cacgttcaat gaaagtcacc
     4381 ctggctgcca tgctgcctat tggatcaagg aagtctgtgg cattccggca ccccaagcga
     4441 tggggtggaa tgagcggggc agtgttggag tccaagcttg agcttgttca gtgaactgcc
     4501 gctgcggagt ctgctcccgt gtcgcctgaa ggcttcactc caacagcagt gttggagttc
     4561 aagcttgagc ttgttcattg aactcccgtt gcggagtcaa cacccgtgtc gcctgaaggc
     4621 ttcactccaa caaaccctca ctcccgcagc caggcggcca tggctttggc gaggccctgg
     4681 ccaacgtgga ggtagccgtc ctgggtgctg tggggtgtgt tccaggcggt ttcgagggtc
     4741 atggcgacgg cgcgggcggg ggtgcggtcg cgcacccagt tgccgctcat gcggtcgcgc
     4801 tcctcctggg tcttcacata ggtggcgaag cgataccttg gcaggatggg cagcggctcg
     4861 cgcatgtgct ggacgcagag ggagagaaag cggtcgtaac tggcgcggcg cgggccggtc
     4921 atttgctcgt agtccatggg gccgaagaag aagggctgct tgtcgccgga gccggggttg
     4981 tgcaggtcga agtaacagcg cagccgcccg acctggatga gggcctcgac gcgctgctgg
     5041 gcggcggcga cttcgggata gacggggcgg gtgtcccagt cgcggttgtg gtcgcgcggc
     5101 acggcttctt tgccaccggc gccgagggcc acgttgtcca cgtccatgat ggggatgaaa
     5161 aagatctcgg tgctcgcgcg cagggcggcg gcctcggggg tgtctccggc cacccattcg
     5221 agaaagccgc gcccgaccca ggaaccgccg gcttcccagg cgtgctggcg ggcgtggacc
     5281 cacacggcct ggggcgcgtc ttttttgccg aggcgcatgc cgggcacctc gcggccttcg
     5341 cgtgtctttg ccagcacgaa gcgctcggca cccggcacgc ggctggcggt ttgctccagc
     5401 agggcttcgg aatgagcagg cagaaagggc ggtccccagg cgagccagaa ggtggcggca
     5461 ggggcggtga tggcataggt ggcgtcggtc ttggtgacgg tgccgggctc ggtgtggcgc
     5521 cacgtttggt catcgctgct gatggcggcg cgcgcgggca gggaccacgc ggcggccaga
     5581 cgctcctgcg cgcggaaggg gcggccggcg ggtttcacac gcacggtgag cgcctgcccg
     5641 ggccgcgcgc catccacgcg gaaataccac cagcacggcc agccgcgccc ctcgcgggag
     5701 acgggtgaga tttcgatcaa accggcgtcc gcatccagcg cgtggacctc cgccgagccg
     5761 ccggggaagt cggaatggag ggtcagcccg gcggaatgaa cgcgcgccgc aatgagcagg
     5821 cacaagacgg gaaggaaaag caggcgcggg agattcatgc gccggagaga acgcgtgcgg
     5881 gcgctttttt ctcacggggc aagggtaaga gggaggctgg tcctccagcc gccgcgagcg
     5941 cgggcactgt gtgccaaagg gctgcgtcat gcacgacacc atcacccatg ccttcggccg
     6001 ctcctggtgg gcgcacgatc aggtcaccag tccgtcaccg cgccatccgg gctgaattcg
     6061 cgcaggacga tctcctgctg gaagggatgc ttcctcacct cggcttcgtt gatggtgatg
     6121 ccgaggccgg gtttcgtgtt cgggcggacg atgcggcctt gtttttcgat gatgaagcct
     6181 tcctcgacca cgtcctgacg ccacggcacg tcgttgtgca cggtttcgca gatgatgtag
     6241 ctcggctgcg agaatccgaa ctcgagcgac gcggcggtgc tgacggggcc ctgcggattg
     6301 tgcggagcca aggcgatgcg atgggcctcc gcgagcgcgg caatgcgttt cgcggcgctg
     6361 aggccgccgc agtgcgtgat gtcgagctgg cagatttcga cggcgcgctt gtcgaacatc
     6421 tctttgaagg cctccaggtt cgtcacgcgc tcgccgctgg ccacgggcgt ggtgagggcg
     6481 gcgttgatct tcgccagacc atcaactgct tccggccagc agggctcctc gaagaagtag
     6541 aggccgaaat catcgagcgc cttgccgaat ttgagcccca tggctggcga aggtcgcgca
     6601 tggcaatcga ccatgatgtc gatgtccggc cccacggcgt cgcgcatggc cgcgacggcc
     6661 gccgcagccg cacggacggg tttcatgccc tcgatgggca tcgtgctggg tacggccatg
     6721 cttttgaagg ccgtgtaacc atccgcaacg gcctgttgag cgagttcggc gaagcgtttc
     6781 gcgttgtcgg cgggcgtttg ataaaagtcc tccatcttcc cgccgccgag atggcagtag
     6841 gtgcgcaccc aatcgcgcac cggaccgccg aagacctttg agagcggcat gttcgccacc
     6901 ttgcctacga tgtcccaaag cgcgagatca atgcccgcga tggccgtggc gcgcacgatg
     6961 ccgtgcccat gccagaagtg ctgccgatac atcatctgcc aaaggtattc cacgcgcgtc
     7021 gggtcctcgc cgatgagcag gtgcgacatg tcttcaatcg cccccacaat gctccgcgtg
     7081 tgccactcca gcgtcgcctc cccccagccg ataaggccgg gctgatccgt gacgactttg
     7141 acaaacaccc agtttcgcat ccgggcatgg cagacaaggg tttcgatggc ggtgattttc
     7201 atgatgaaag gtggtgtgtg tcaggaatga atggaatatc tccagaccag aattggtcga
     7261 gcttacgcca agcagcagca ctagcaggca gcaagtaggg aagaaactcg tttggcgata
     7321 cttcagagtg tccccagaca ttctgaatgc cttcaagaat tccaatcgtc gcaaactctc
     7381 gcgtggctgg atcatcatca gtatgcagac cttcgataaa aatggcgacg gcaccgaact
     7441 cgttggtttt ctccaagcgc tgaagctcca aaaggtgcct cgcaaaggca gcagcgtgaa
     7501 ggtagagaag ctctgacccg ttttcagtgc aggattcgac gaaagcagtt ctgaacgaag
     7561 gacatgcttc caaccagagg ctgacaatgt tcgccttatt cattgtcatc agattcgctt
     7621 cgcaatgacc tcaatcaact gctccaagct ccccgcagcg acgacggcaa tgctctcttg
     7681 ataaatgccg gtgtcgtaaa gctcgcgagg tggcagatag ctggcccccc agtcggcggc
     7741 gatggtcgaa acaatgatga ttttgttggg gaatcgccgg cggagctcgg tttggaggag
     7801 gttgtagctt tcgccttgga cgccgagcca gacggcgtcg ccgatgtgcc agaggacgac
     7861 ttggagcggg tagatgtcgc cgggtggaag ctggtggagg cgatggagga ggcgggtttt
     7921 gcgttcggcc atggcgcggc agtcggcggc gcgagtttca tcgccagcgg cgcgggcggc
     7981 ggcttcggcg gccttgaatt gatcgagttc ggcctgggtt tgttcgatcg tcggctgtcc
     8041 gggacggtac ttcagcggtt cgtgccagcg ctggagcttc cacacggctt tttcggccag
     8101 atcgattttt tgatgcttcc acgagccaag tgtcgcgccg gagacaatcg ggccttgata
     8161 gacaaatgtc gttccagcag cgggaagagc tgtgagggca gacaaagcgg cgtagccgag
     8221 ttcgcgtccg tttttgtccg ccacagcggt gtcgccgacg aagccttcgc gcgggccgag
     8281 ttcgccgctg gcaccttgca ggaacaaaca aggagcgtcg gtgtcgcgtt cgacgggttc
     8341 gcgcatcgcg ccgatgtagt cggggctgat gagcgtgttc tcccacgcga gtgtggtggg
     8401 atggcaggcg tagttcacga tgctggcgag catttggccc gcatcatcgg tcacgcgagc
     8461 gacgatgacg gtgtcgtcag cgggcacgcc gggattgtgg ccgcatacga actgcttccc
     8521 gtcccagaaa tcgcgatgtg tggctaacga gcacctgcca cggccataaa cgatgcctgc
     8581 gggcttcaac gaagcgatgg catcgcggat caaagcgccc gctttttctc cgagtgactg
     8641 caaatacggc ggaatcagct ctccaccggg caaggaggca cgatccaacc ccatcaggcc
     8701 cgcgccgtgc gtatgcgaga agacgacaag gatctcctct ttcgccacac cgccgatctc
     8761 cgcagcctgc gcgacgagtt tcgcatgctc ggttgctccc atcacgcaat gatcgagcgc
     8821 gagcacgatt tgccgctcgc tgccttccat cggcgcaaag acggtgacgg tggctcgcag
     8881 cggccgatgc acgccggtgg cgcgttcatg cttcgctgcg ccccacatgc ggtggtagat
     8941 gtccgcgggc ggcgtgatgt cacaccactt caacgcgaag cggcagcggc tctgcggcgt
     9001 ggggtcttcg gtgcgggaaa gggcgggcat gcgctctcaa agacacttca tgggaccaac
     9061 tttcaaagga caaacactct ctcgagatcg cgtagtttgt gcatgaggcc tttcctgctc
     9121 ctttttgccg ccatcaacac ttttgctgcc gatccaatcg ccatcggctc gcgtcgtgag
     9181 ttgttcgtgg atgatctcct gatcgaaaaa ctcagcgtca aggctgagca acggctgcat
     9241 cattcgcagg cgaaggagat cgccatcaag cacgatgcgc cctgggaggg cagcggcacg
     9301 gggtatcaca gcgtcttcaa ggacggcgac aaataccgca tgtattacaa ggcgtggcag
     9361 ctcaccgtca caccacctgg aaaagtgaac acgggcgagc atccgctctt cacctgctac
     9421 gcggagagcg atgacggcat tcattggcgc aagccggagc tcggtttgtt cgagttcaaa
     9481 ggctcgaagg ccaacaacat cgttctcgct ccgggaaaga tgggcgcggc gaatcctgac
     9541 cccggtcaca tcgcgatctt caaggacgac aatcccgccg cgaagcccga ggcgcgctac
     9601 aaggccatcg tgcgctcggg ttcgccgaag ggcctgctcg cgtttcaatc gccggatggc
     9661 ctcaagtggt cgccgatgca ggagacgccc gtgatcacgg atggtgcgtt tgattcgcag
     9721 aatctcgcgt tctgggatgc gcacagcggc gtgtatcgcg cctactggcg ctacttcacc
     9781 ggcggcacga cggacgagaa gaattggaag ccccccggct tccgcgccat ccgcaccgcc
     9841 acgtcgaagg attttcttca ctgggagaat cagcagaacc tgagctacat tgattcaccc
     9901 gacgaggcgc tttataccaa tcaagtgaag ccgtatgcgc gtgcgccgca actgctgttc
     9961 ggttttccca cgcgctacat cgagcgcggc tggtccgaga gcatgcgcgc ccttccggag
    10021 cgcgagcacc gcgaatggcg ctcgaaggcg aatgatcgct acggcatggc tgtcacagaa
    10081 ggtttgttca tggcaagccg tgatggtgtg aaattcaaac gctggaacga cgccttcctg
    10141 ccgccgggca tcgagcggcc cggcacctgg aactacgggc agcaatacat cgcctggcat
    10201 ctggtggaga caaaatccgc gctcgaaggc gcgccagatg aactctcgct ctacggaggc
    10261 gaaaactact ggaccggcac cagcagcgag ctgcgtcgtt acacactgcg tctcgacggt
    10321 tttgtctcca tccacgcgcc gatgagcggc ggcgaattga tcaccaagcc gttcaccttc
    10381 accggcaaga cgctcacgat gaactttgcc acctccgctg ccggtggcat tcgcgtcgaa
    10441 attcaggatg caaatggcaa agccatccct ggcttcacct tggacgattc cgaagaacac
    10501 ttcggcgact ccatccagcg caccgtgttt tggaaaagcg gcaatgatgt gagcgctctc
    10561 gccgggaagg cagtgcggct gcgctttgtg ctcaaagacg cggaccttta cgcgatcaag
    10621 tttgagtgaa tggcggattc acatttaggt cttgagaggc aaccgcgtgg cgcggctttc
    10681 tgccctgcga atttgcgtga tctcgcggct tgtgatcttc gccgccagtc cgtagcatcg
    10741 gagataattc acccccaaaa tcctcatgat gccgcacttc cgccgatggg ttgtcacttc
    10801 cgcgctgttt tgcgcgggcg tgtttctttt gaatgcgcag cagcccgcct ggcgctcctg
    10861 gaaaagcacg gacggccggg tggtcgaggc tgtgttcctg ggtgttgaag cgggtggtgt
    10921 gaagatcaag acccgcgccg gcgccgagtt cgtcgtgccg ctggagaggc tctccgccga
    10981 ggatcaggcc tgggtggcgc agcagccggc ggcggcgccc cccggc
//
LOCUS       DAOMYJ010000178        10973 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Prosthecobacter sp. isolate AMR_MDS_5629
            NODE_1988_length_10973_cov_20.121542, whole genome shotgun
            sequence.
ACCESSION   DAOMYJ010000178 DAOMYJ010000000
VERSION     DAOMYJ010000178.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867850
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prosthecobacter sp. (wastewater metagenome)
  ORGANISM  Prosthecobacter sp.
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Verrucomicrobiaceae; Prosthecobacter.
REFERENCE   1  (bases 1 to 10973)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10973)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.30
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:34:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,742
            CDSs (total)                      :: 4,684
            Genes (coding)                    :: 4,676
            CDSs (with protein)               :: 4,676
            Genes (RNA)                       :: 58
            tRNAs                             :: 55
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10973
                     /organism="Prosthecobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1988_length_10973_cov_20.121542"
                     /isolate="AMR_MDS_5629"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1965333"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..481)
                     /locus_tag="PLP58_16220"
     CDS             complement(<1..481)
                     /locus_tag="PLP58_16220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ10126.1"
                     /translation="MTAKTSPEVKPWVRRVAEAAGMAPESAASEARLGWWGWEMRKFL
                     NFCQGLPAGTALMEALAGYGRFLNANDPPPEEWRKEQAREALRCFKKGIENWAILPPD
                     ESGKVEVRFRARTIQEAADGAEIPDSARIAGTTDGAGSPDDAARRGAQEGGEGAEALL
                     "
     gene            complement(625..1164)
                     /locus_tag="PLP58_16225"
     CDS             complement(625..1164)
                     /locus_tag="PLP58_16225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ10127.1"
                     /translation="MNAAPTTPALANDFHAAVIGSTALLGHGVGRRQLGRFWHECRDG
                     DDGAREVYDRHYSRYHYKDGRQPKLFVGPGEKMVLITETGDAIWCWRKFRDASGQQGI
                     NNAIFRNEGMILSSLLILDAEELAWKRWPGERLYTYVKPTGIRSTNPGACYKKAGWRV
                     CGVTKWNKLVILEKCPNVV"
     gene            1800..2330
                     /locus_tag="PLP58_16230"
     CDS             1800..2330
                     /locus_tag="PLP58_16230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ10128.1"
                     /translation="MSTAIDFGIAAYESTVATLEPQIDYGPDGESQDEPWTDTPKFRA
                     WLEALELGDDNTEALLGIVPSVSLAMNHGPLLGIESFIEDNSESGHKVWEAGFLIFAT
                     GPNGDFVVVDTRNGSGQTGWLPMAMIWGMDADKVREHFVPTNPNLGDFVRASEEEWAT
                     VPKDWYDARDQAAKTN"
     gene            3103..3660
                     /locus_tag="PLP58_16235"
     CDS             3103..3660
                     /locus_tag="PLP58_16235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ10129.1"
                     /translation="MKQQSMKFVALICLLSFMPRLGCARDPEKVELWRLEQFLAADWK
                     ALSFGFESSHGDYYSVDQSIVLQRLPDGTVSLSIVKDGQHDLPVVKRILPFARAQEIQ
                     KAVADFHRKVLGEVSIQEWVDTHKGDERLALIEKHFPHGYTYRVMALRITKSEGETVR
                     LSKNFDERGGTLKAFTAYFDALLKK"
     gene            complement(4386..9587)
                     /locus_tag="PLP58_16240"
     CDS             complement(4386..9587)
                     /locus_tag="PLP58_16240"
                     /inference="COORDINATES: protein motif:HMM:NF019325.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HRJ10130.1"
                     /translation="MKPDTRPNRSLFRITGQSLAALALGSLIPFVPPAQAADFTADNL
                     IVERMGDGTAALGSGATAVSLVEYSPTGTEAQVINLPTTGADRLTDAGSSTSNGYLGS
                     QGAYLAVPGYNVDAGTASVANANSKVTSIFGVNGSVTTRTLHPADATIFGGSNYRSAW
                     PVDSNKFYAAGTGSTTTGGVWYYDGSAFSQLLSGNIRIVGIYAGQLYYSTGSGTQGIY
                     KVGTGTPTSTAAPSLFQASTSPYGFVIFDTDNDTQPDILFIADDGTGAAAGLRRFDWN
                     SGSSIWVETYSRRLVAATGLLTTATSSVISVRGLTGSYSGGTATLYATTTETSNNRLV
                     KIVDDGTTTPTTFTTLAQAGANRAFRGVAFAPVSANAPPNIGTEPLSQTIQSGTTAGL
                     VVAASGTEPFTFQWYQGASGDTSTPVGTNSPNFVTPSLTMTTSYWVRVTNAHGSDDSA
                     TAVITVTTLPVVLSTVPANAAANVPVDAVITVNFSKSVNLSASAITVVDAMSNPVAFT
                     GLPIEGVESVEITPVDDLAYNETYTVTVVAAQVEDEDMNAMAENHVFTFTTEPMVAPA
                     FTTHPAATTNIASGGQATLNVVATGKPAPSIQWYQGATGDTSTPLSGENGTSFTTPAL
                     TASTQYWARATNPLSSASSNTANVNVVSPDLVVELSAPGSVGVGENITFTLRARNTGL
                     VSATNVSVVFYLPSGLAYLSDDPGAGNGFTSSYPPGAVQFAGGMLGANETAVLTVTAS
                     HSGAGIYTLPAGSAVIDPASIVSESNETNNDSAEISTSVLAVLGSTPVPGDVIFNEYM
                     SDNDSNGNDFIELLVLTDGVDLRGLRISDNELITSSGVQTLNTNEAVYVLGTDPYLAN
                     VPAGVTIAIWALATGVTTDTVADPFASDWSMTLCPGTGVTATTDGLGGSLNLGLAAGG
                     EALYLYLPGPDGSSAGDDNIYLDFVSFESDGGEAPAGLVDINLPSIADNAYYTGNTAA
                     GNDLAANWTRYDGAPNASTTAGEANPGQDLSGLRIVPTAGSIAFVSATFTVNQGDLVA
                     NISLERTGGTLPTSVTLSTADGMASISPPFSPALAGTDYEALTTLVEFALDETVKVVP
                     VTLTPRSGTKIPNLQFDAALSAPAGGATLGATDTASVRILTADVTAPKITVKTPAAPR
                     APATSLVTSVQWPYQLSGTAGDALGIDRVEITVNGGAPILATLGASSKPTSVPFTAAI
                     EPDEGINTVVFTAYDLKGNSTSLTRVIDFSRRYELALARTLSGSPDTSAGSIALYATP
                     KADATAPAPKYDAAQTLSVLPGTSILLQAKPLSGFVFSHWSGAPAGAMIHGDRLSFVM
                     PASNVPAITAHFLPNPFLGLGDKPVFYGLLTPESGTALGNTTFGFLTGALTTGKGGFS
                     GKILLDGKTTGFAAVVFGNGSVWFNVAGALLDTLVFDGGKELSLTFASGKLEAVLVNG
                     SDGSSGDIFPAIYSKTSTLAGSPLLNRPAKAGDPVNQGYYTVVLESKAQTPPRDSDTY
                     PQGDGYTSLTLAVDGTFKFAGVLADKTKVTGASALVAGHTSPVFAQLLTPGAATKNAT
                     FGGVLAFDTAPADTDVSGQDLVWIRPAVTETATLATQLYTDGWPEGITVDAFGALFAT
                     SDIESSLGLGSPSPIGNSSFVFSGGKLAANLEKVVNIAVNTTTPAITGDKSIKAMISA
                     KTGIFTGAFTPNWLPLDTKQPAHAGILLLKGANKGGYGHFISNIPGDTNPASGAVSLG
                     VPLHTLP"
     gene            complement(9717..>10973)
                     /locus_tag="PLP58_16245"
     CDS             complement(9717..>10973)
                     /locus_tag="PLP58_16245"
                     /inference="COORDINATES: protein motif:HMM:NF025017.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HRJ10131.1"
                     /translation="SLIRAAKYCPEPNIPPPPRNRTEAIVAAAPGAPVHVLLDDEEAE
                     HLPGCNLAVRRRALEEIGGFRAQYRAAGDDVDVCWRLRAAGGRLRYVPGAMVWHHRRY
                     QTRAYLRQQAGYGYAEALLMKDHPRRFGMLGGARWTGGIYGDQSGGEIESGDIFHGSQ
                     GLGLFQGIYQTGPRCWADWLGGLAWVALAAGLLVCGQAAAALGVFVFSWLLALCRARS
                     LPPAPFRLTLRERLTRLALCWLQPMVREGARLRGMVETGAFPGGWPEWDTAWPAPCDH
                     RRALPAGELHLWSESGPRRGRFLREYAALAASQHDAVRVDDGWQSFDVELQPDAHFSP
                     AVITTEEYHGRGRCLLRVRLLWRASSAAWLVLAAVLLACAVTPGIKPLLFLCCAHFGF
                     RQHEAWQRVKTVMGRIRAAPGGREAE"
BASE COUNT         2068 a         3301 c         3501 g         2103 t
ORIGIN      
        1 gcagcagcgc ctccgcgcct tccccgccct cctgcgcgcc gcgcctcgcc gcgtcatccg
       61 gtgaaccggc gccatccgtg gttcctgcaa tgcgggctga atccgggatt tccgcgccat
      121 ccgccgcctc ctggatcgtc cgcgcccgga agcgcacctc cactttccca gactcatccg
      181 gcggcagtat ggcccagttt tcgatgccct ttttgaagca ccgcagcgcc tcgcgcgcct
      241 gctctttccg ccattcttcc ggcggcgggt cattggcgtt caaaaaacgc ccgtagcctg
      301 ccagcgcctc catcagtgcg gtgccagccg gcagtccctg gcagaaattc agaaacttcc
      361 gcatctccca gccccaccac cccagccgcg cctcgctggc cgccgactcc ggcgccatcc
      421 ccgccgcctc cgccacccgc cgcacccagg gtttgacttc cggactcgtt tttgctgtca
      481 tggacatata aagaaaaatt tgatgaaatt gagccgccgc gccaaatcac aacttcgaaa
      541 tgccgctctc catttatgga cgaattttcc accgcttcaa cacagaacca ttgacgaatc
      601 ccgctcgccg atgcattcgt gtgtctaaac aacgttaggg cacttctcaa gaatcacgag
      661 cttgttccat ttcgtgactc cacagactcg ccatcccgcc tttttgtagc acgcgccagg
      721 gttcgtgctg cggatgcccg ttggcttgac gtaagtgtaa aggcgttctc caggccaccg
      781 cttccacgcc aactcctcag cgtccaagat gagcaggctt gagagtatca ttccctcgtt
      841 gcggaagatc gcgttattga ttccttgctg gccggatgcg tccctgaact tgcgccaaca
      901 ccagatcgcg tctccggtct ccgtgatgag caccatcttt tcccccggcc cgacgaacag
      961 tttcggctgc ctcccgtcct tgtagtgata ccgcgaatag tgacgatcat agacttccct
     1021 tgctccatca tctccatcac gacactcatg ccagaagcgt cctaactgcc tgcgtccaac
     1081 gccgtgccct aacaatgcgg tgctgccaat gactgccgcg tggaagtcat tggcgagggc
     1141 gggcgtggtg ggcgcggcat tcatggcaga ccttgatcgt tcgcccaaag aatccgaccc
     1201 gcgatgaagc tcgaaacaga accacaacga gttttggtcg gcagtcggtg gtggcggttt
     1261 gcagagcgag tgacggatgc cctgcaactc ggggaccgag tgttcgtcat tttcgactac
     1321 atgggatttc ctcaggggtg tccggccccg aacctgagag cctacgacct gcaccagaaa
     1381 cttctttgga ctgcagcaca tccgattgac cagccagccg cagcatatgt gaactttctc
     1441 tctgagacat cacttgtagc ttggaacttt gcttgctttc gatgcacttt ggatgaggcg
     1501 tcgggcgaga ttatcgaggc tcagtttacg aagtagctat gattacaacc cacgaaatac
     1561 acggccaacc aagggcgaac aaggcgcggc tggacaaccg ctggggctgc tctgtcggcc
     1621 acgctctccg taactttaac ccgctatccc gtttcaacgc tcacccccgc cccagcgggg
     1681 gccagcgctt gaacgttcgc tccaaaaaaa tgctgactcg gtcactcatg ctctttggct
     1741 gctttgcgtt acttggctgc gaccgggaag ctacaacacc ggaaagttca acaaaaccca
     1801 tgagcacagc cattgatttc ggtattgctg cctacgagag cacggttgcc acgttggaac
     1861 ctcagattga ttacggcccg gatggcgaga gccaagacga gccctggact gatacaccca
     1921 agtttcgggc gtggcttgaa gcgcttgaac taggcgatga caataccgaa gcgttgcttg
     1981 ggattgtgcc ctcggtttcg ttggcgatga accatgggcc actcctcggc attgagagct
     2041 ttatcgagga caactcggaa tcgggccata aggtttggga ggctggcttc ctaatctttg
     2101 cgactggacc caacggagat ttcgtggtgg tggacactcg caatggaagt ggacagactg
     2161 gatggctgcc gatggcgatg atttggggta tggatgcgga caaggttcgc gagcatttcg
     2221 tgccgaccaa tccaaatctt ggcgatttcg tccgcgcctc tgaagaggag tgggcaactg
     2281 tccccaagga ttggtatgat gcgcgagatc aagccgccaa aaccaactga gcgaacaagg
     2341 cgtcgctctc aacacctgcc ccgccgcgag ttcaatccgc catgactatt caaccatcaa
     2401 cccacagtcg aagccgcgcc cccgggcagg tgtgagagga cttcgacgtt cggccaagta
     2461 atggagccca gtgccatgag attgaagcgc acattaatct tcaccgtgct tttggcaggg
     2521 ctcggctatc tcatctacac tgcgcttccg ccttcctccg atggtctagc cgctgttgag
     2581 aagcttgcta ctgttgatga gttctctctg gggcacgtcg gtttgagagg ccccattccg
     2641 aagcgtgaag actggtttat caccattctt cggagtaggc atgcagaccg attgttctct
     2701 ctactttaca ggaggggcac acctgccgcc cgctcttatg ctctggctgg actgaggttg
     2761 accgacatgt ctacatatag acggtgtgca gccgactact ctgcgaccac cgtcaccctc
     2821 cgcacggctg gagggtgcta cgtgcacgaa ggcgtttctc cggtgagcat tgttcaggct
     2881 tttgacagcg gagcagtgga ggactacatg aaggaccgcg atcggcttta catgaactgg
     2941 ccacatgagt agagaacatc acaatccaca accaaggccg aacaaaacgg atgcaggcaa
     3001 cggctcgaag gctatctgtc gtgtcagcaa tgtcttgcgc tcgccgtcgc ctgatccgag
     3061 acgttcgccc aactaacagc acccgttcag tcgatgtgcc acatgaagca gcaatccatg
     3121 aaatttgtag cgctgatctg cctcctgtcg tttatgccga gactcggctg cgctagggat
     3181 ccagagaagg ttgagttgtg gcgtctggag cagttcttgg cggcagactg gaaggccctt
     3241 agctttggat ttgagagttc acatggcgac tactactctg tggatcagtc catcgttctt
     3301 cagcgactgc ccgatggcac agtctcctta tccatcgtga aggatggcca gcatgacttg
     3361 cctgttgtga agcggattct accttttgcc agagcgcaag agattcagaa ggctgtggct
     3421 gattttcatc ggaaagttct tggagaggtt tcaatacaag agtgggtgga tacccacaag
     3481 ggtgacgagc gactggctct aattgagaag cacttccctc atggctatac atatagagtc
     3541 atggctttgc gtatcacgaa gtcggaggga gagacggtgc gattgtcgaa gaactttgat
     3601 gagcgggggg gcactttgaa ggcgttcact gcctattttg acgccttact aaagaagtag
     3661 attggctcag catgactact gccacgacta cagccggaac gagggcgaac aaaacggatg
     3721 caggcaacgg ctcgaaggct atctgtcgtg tcagcaacgt cttgcgctcg ccgtcgcctg
     3781 atccgagacg ttctgcaaac taaccgacgc tcgccgcgct tgaccaactc caaagagacc
     3841 ttgggccgca gtcatcggtt gagcattcag cattgggaac tcagatgcca gaactcaact
     3901 cagatgccat ttaggaccgc gcttgttttc ggtttgggct tccgccattc ccagggcggg
     3961 acgggcgcgg ggtcaatgcc tctggaggat ggcgggcagg gtcgaaggat gatccgcgca
     4021 atgcgcggcg catccagcca ccatgcgcgg cggctgaagc ctggtcacca acgggggtgg
     4081 ggaagatgga gaggccatga gagggaggat gaccactggt ctggcgcgct ggcttttatt
     4141 tctcgaagaa aagcgatccc atcagaacgg gcgggaaatg ccgggaagat ttttttcgcg
     4201 aaggcaaagg cgacggtgtg ttttcatcca cttgcttcca cccggggaca ggcagccgcc
     4261 tctttgcgca gtcagcaagc ctgcgcccca atattccgag ttttaaggag tcccccctct
     4321 ggacatggca aaaggccgtc ctccttgaag aggacggcct ttgatccggg gggctgatgc
     4381 gcgcgtcagg gcagcgtgtg caatggcacg cccaggctga ccgcgccgct tgccgggttg
     4441 gtgtcgccgg ggatgttgct gatgaagtgg ccgtagccgc ctttgttggc gcctttgagc
     4501 agcaggatgc cggcatgggc gggctgcttg gtgtcaagcg gcaaccagtt cggggtgaag
     4561 gctccagtga agatgccggt cttggcggag atcatggctt tgatgctctt gtcgccagtg
     4621 atggcggggg tggtggtgtt caccgcgatg ttgaccactt tctcaaggtt cgccgccagc
     4681 ttgccaccgg agaagacaaa ggaggagttg ccaatggggg agggactgcc gagtccgagg
     4741 gaggattcga tgtcgctggt ggcaaagagg gcgccaaagg catccacggt gatgccttcc
     4801 ggccagccgt cggtatagag ctgggtcgca agggtggctg tctctgtgac ggcggggcga
     4861 atccacacaa ggtcctgacc gctgacatcg gtgtccgcag gggctgtgtc gaaggcgagc
     4921 acgccgccga aggtggcgtt ctttgtggcg gcgccgggag tcaggagctg ggcaaagaca
     4981 gggctggtgt ggcccgcgac cagggccgag gcgccggtga ccttggtttt gtccgccagg
     5041 acaccggcga acttgaaggt gccgtccacc gcgagtgtga gggaggtgta gccgtctccc
     5101 tgcggatagg tgtcgctgtc cctgggcggg gtctgggctt tggattccag caccaccgtg
     5161 tagtagccct ggttgaccgg gtctcccgcc ttggccgggc ggttcagaag gggggagccc
     5221 gccagcgtgg aggtcttgct gtagatggcg gggaaaatgt caccgctgct cccgtcactg
     5281 ccgttcacca gcacggcctc cagcttgccg gacgcgaagg tcaggctcag ctccttgccg
     5341 ccgtcgaaca cgagggtgtc gagaagcgcc ccggcgacgt tgaaccagac gctgccgttg
     5401 ccaaagacca cggcggcaaa tccggtcgtt ttgccgtcga gcaggatttt gccgctgaag
     5461 ccgcctttgc cggtggtcag cgcgccggtc aggaagccga aggtggtgtt gccgagggcg
     5521 gtgccgcttt cgggagtcag gaggccgtaa aacaccggct tgtctcccag gccgaggaag
     5581 gggttcggca ggaagtgggc ggtgatggcg ggcacgttgg aggcgggcat cacaaacgag
     5641 agcctgtcgc catggatcat cgcccccgcg ggagcgccgc tccagtggct gaagacaaat
     5701 ccggaaagcg gcttggcctg gagcagaatg gaagtgccgg gcagcacgga gagcgtctgc
     5761 gcggcgtcat acttgggcgc gggcgcggtg gcgtccgctt tcggagtggc atagagggcg
     5821 atgctgccgg cgctggtgtc ggggctgccg gagagagtgc gcgccagggc aagctcgtag
     5881 cggcgtgaaa agtcaatgac gcgggtgagt gaagtggagt tgcctttcag gtcgtaggct
     5941 gtgaaaacga ccgtgttgat accctcgtcc ggctcaatgg ctgccgtgaa gggcacggag
     6001 gtcggtttgc tggaggcgcc aagcgttgcg aggatcggag ctccgccgtt cacagtgatt
     6061 tcaacgcggt caatgcccag cgcgtcgccg gccgtgccgg agagctggta cggccactgc
     6121 acgctcgtaa cgaggctcgt cgccggagcg cgcggagcgg cgggagtttt gaccgtgatc
     6181 ttcggcgctg tgacatcggc ggtcagaatg cggacactgg cggtgtcggt ggctccgaga
     6241 gtggccccgc ctgcgggggc gctcagcgcg gcgtcgaatt gcagatttgg aatcttcgtt
     6301 cccgaacgcg gagtgagggt cacgggaacc accttgaccg tctcatccag cgcgaattcg
     6361 acaagggtgg tgagagcctc ataatccgtt cccgcaagcg ccggtgagaa gggcggcgag
     6421 atggatgcca tgccatcggc ggtgctcagc gtcacgctcg tgggcagggt gccgcccgtg
     6481 cgctcgaggc tgatgttggc caccaagtcg ccctggttca cggtgaatgt ggccgaaacg
     6541 aatgcgatgc tgccggcagt ggggacgatg cgcaggccgc tcaagtcctg gccgggattt
     6601 gcctcgcccg ctgtggtgct ggcgtttggc gcgccgtcat agcgcgtcca gttggcggcc
     6661 agatcattgc ccgcggctgt gttgccggtg tagtaggcgt tgtcggcgat tgagggcaga
     6721 ttgatgtcca ccagccctgc gggtgcctcg ccgccatcgc tttcaaagga aacgaagtcg
     6781 agatagatgt tgtcatcgcc cgcgctgctg ccatccggtc cgggcaggta aaggtatagg
     6841 gcctccccgc cggctgccag accgagattg aggctgccgc cgagcccatc cgtcgtggcg
     6901 gtgactccgg tgcccgggca gagcgtcatg ctccagtcgc tggcaaaggg gtccgccaca
     6961 gtgtcggttg tcacgccagt ggcgagcgcc caaatggcta tcgtgacgcc tgcgggcaca
     7021 tttgccagat acgggtcggt gccgaggaca taaaccgcct cgttggtgtt gagtgtctgc
     7081 acgccgctgg aagtgatgag ttcgttgtcg gaaatcctca gcccgcgcag gtccacgccg
     7141 tcggtgagca cgagcaactc gatgaagtcg ttcccgttgc tgtcgttgtc ggacatgtac
     7201 tcgttgaaga tcacatcacc gggcacgggc gtggagccga gcacggcgag caccgaggtt
     7261 gagatttccg cggagtcgtt gttggtctcg ttggactcgc tgacgatgct ggccgggtca
     7321 atgaccgccg aaccggcggg gagcgtgtaa atgccggctc cagaatgcga ggcggtgacg
     7381 gtgaggacgg cggtctcgtt cgcgccgagc atgccgccgg caaactgaac cgcgccaggc
     7441 ggataggagg aggtgaagcc atttcccgcg cctggatcat ccgagagata agccaagcct
     7501 gaaggcaggt aaaacacgac ggagacattc gtggcggaca ccagacccgt gttccgggcg
     7561 cgcagggtga aggtgatgtt ctcgcccacg ccgacgctgc cgggagcgga aagctccacc
     7621 accaggtcgg gggagaccac attcacattc gccgtgttgc tgctggcgct ggagagaggg
     7681 ttggtggcgc gcgcccaata ctgggttgaa gcggtcaatg ccggcgtcgt gaagctggtc
     7741 ccgttctcgc cggaaagagg cgtgctggtg tcacccgtcg cgccttgata ccactggatg
     7801 gaaggcgcgg gttttcccgt ggccacgaca ttgagcgtgg cctggccgcc ggaggcgatg
     7861 ttggtggtgg cagcgggatg cgtggtgaaa gccggagcca ccatgggctc ggtggtgaag
     7921 gtgaacacat ggttttccgc catcgcgttc atgtcttcgt cctcgacttg cgcggcgacg
     7981 acggtcacgg tgtaagtctc gttgtaggcg agatcgtcca ccggggtgat ctcgacactc
     8041 tccacaccct cgatgggcag cccggtgaag gcgaccggat tgctcatggc gtccaccacc
     8101 gtgatggcag acgcgctcag attgaccgac ttgctaaagt tcacggtgat gaccgcgtcc
     8161 accggcacat tagcagcggc attggccggc acagtggaaa gcacgacggg aagcgtggtg
     8221 acggtgatga cggcagtggc gctgtcatcc gaaccgtggg cgttggtcac acgcacccag
     8281 tagctcgtcg tcattgtgag ggaaggagtc acgaaattcg gcgaattcgt ccccaccggc
     8341 gtgctcgtgt cgccactggc tccctggtac cattggaagg tgaagggctc cgtgcccgat
     8401 gccgcgacga ccaagcccgc cgtggtgccg ctttgaatgg tctggctcag cggctcggtg
     8461 ccgatgtttg gcggcgcatt ggcggacact ggcgcgaaag ccacgccacg gaacgcgcgg
     8521 ttggctcccg cctgggccag agtcgtgaag gtggtgggcg tcgttgtgcc gtcgtccacg
     8581 atcttcacca gccggttgtt gctcgtttca gtggtcgtcg cataaagcgt ggccgtgccg
     8641 ccggagtagg agccggtgag gccgcgcacc gagatcacac tgctggtcgc cgtggtcagc
     8701 aggcccgtgg cggccacgag acgccggcta tatgtctcca cccagatgct ggagccggag
     8761 ttccagtcaa agcgccgcag tcctgccgcg gcacccgtgc catcgtccgc gatgaacagg
     8821 atgtccggct gcgtgtcgtt gtcggtgtcg aaaatgacaa agccatacgg gctcgtgctc
     8881 gcctgaaaaa gcgagggcgc ggccgtcgag gtcggcgttc ccgtgcccac tttatagatg
     8941 ccctgcgtgc cggagccggt ggagtaataa agctggcccg cgtagatgcc gacgatgcgg
     9001 atgttgccag acaaaagctg gctgaaggcg ctgccgtcat aataccagac accaccggtt
     9061 gtcgtggagc ccgtgcccgc cgcgtaaaac ttgttgctgt ccaccggcca cgcggagcgg
     9121 taattgctgc cgccgaaaat cgtcgcatcc gcgggatgca gcgtccgggt ggtgacgctt
     9181 ccgttgacgc caaagatgct ggtcaccttg ctgttcgcat tcgccaccga cgccgtccca
     9241 gcgtccacgt tgtaacccgg gactgcgaga tacgcgccct gagatcccag atagccgttc
     9301 gaagtcgagg agccggcatc cgtcaggcgg tccgcaccgg tcgtgggcag attgatcacc
     9361 tgcgcctccg tccccgtcgg cgagtattcg accaaagaaa cagcggttgc ccccgatcca
     9421 agggccgccg tgccatcccc catgcgttcg acgatgagat tatccgcggt gaaatccgcc
     9481 gcttgcgcgg gcggaacgaa tggaatcagg ctgccgagag cgagcgctgc gagggattga
     9541 ccggtaattc gaaacaagct gcggtttggg cgggtgtctg gtttcatgat tggaaaacgg
     9601 gtgcggaaac cgagcatgag ccgcgccagc actggcaggc aatcctgaaa gcgtgacttt
     9661 tccgacaatc tatttcgact cgttatggct gggtctggtt attcgagaaa tcgccgttat
     9721 tcagcctccc tgccgcccgg tgccgcacgg attctcccca tgaccgtctt gacccgctgc
     9781 caagcctcgt gctgccggaa cccaaaatgc gcgcagcata aaaaaagcag cggtttgatg
     9841 cccggcgtca cagcgcaggc caggagcacg gcagccagca ccagccatgc ggcgctggag
     9901 gcacgccaga gcaggcgcac gcgcagcagg cagcggcccc ggccatggta ctcctcggtg
     9961 gtgatcaccg ccggagaaaa atgcgcgtcc ggctgaagct ccacatcaaa agactgccag
    10021 ccatcgtcca cgcgcacggc atcatgctgg ctggcggcca gcgcggcata ctcgcgcagg
    10081 aaacgccccc gccgcggacc cgactcgctc caaaggtgca gctcccccgc gggcaacgcg
    10141 cggcggtggt cgcatggcgc gggccaggcc gtgtcccatt caggccagcc tccggggaag
    10201 gcgcccgtct ccaccatgcc gcgcaggcgc gcgccctccc gcaccatggg ctgaagccag
    10261 cacagcgcca ggcgcgtcag cctttcccgc agcgtcaggc ggaaaggcgc cggcggcagg
    10321 gagcgcgcgc ggcagagcgc cagcagccag gaaaaaacaa agacacccag cgcggcagcc
    10381 gcctgcccgc agaccagcag ccccgccgcc agcgccaccc aggccaggcc gcccagccag
    10441 tcagcccagc agcgcggccc ggtctggtag atgccctgga acagacccag cccctgcgaa
    10501 ccgtggaaga tgtcccccga ctcgatctcc ccgccgcttt gatccccgta aatgcccccc
    10561 gtccagcgcg cgccgccgag catcccaaag cggcgcggat ggtccttcat cagcagagcc
    10621 tcggcgtaac cgtaacccgc ctgctggcgc aaatacgcgc gtgtctggta gcggcggtgg
    10681 tgccacacca tcgcgcccgg cacataacgc agccgcccgc ccgccgcccg cagccgccag
    10741 cacacatcca catcatcccc ggcggcgcgg tactgcgcgc ggaagccgcc gatctcctcc
    10801 agcgcccgcc ggcgcaccgc cagattgcag cccggcagat gctccgcctc ctcgtcatcc
    10861 agcagcacat gcaccggcgc gcccggcgcc gcggccacga tcgcctccgt gcggttgcgc
    10921 ggcggcggcg ggatgttcgg ctccgggcag tactttgccg cccggatcag gga
//
LOCUS       DAOMYJ010000179        10954 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Prosthecobacter sp. isolate AMR_MDS_5629
            NODE_1994_length_10954_cov_19.209744, whole genome shotgun
            sequence.
ACCESSION   DAOMYJ010000179 DAOMYJ010000000
VERSION     DAOMYJ010000179.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867850
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prosthecobacter sp. (wastewater metagenome)
  ORGANISM  Prosthecobacter sp.
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Verrucomicrobiaceae; Prosthecobacter.
REFERENCE   1  (bases 1 to 10954)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10954)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.30
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:34:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,742
            CDSs (total)                      :: 4,684
            Genes (coding)                    :: 4,676
            CDSs (with protein)               :: 4,676
            Genes (RNA)                       :: 58
            tRNAs                             :: 55
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10954
                     /organism="Prosthecobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1994_length_10954_cov_19.209744"
                     /isolate="AMR_MDS_5629"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1965333"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..401)
                     /locus_tag="PLP58_16250"
     CDS             complement(<1..401)
                     /locus_tag="PLP58_16250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ10132.1"
                     /translation="MTKVQTPVNSGAAHPRSSAGGNPFSADVCTGPFDLEAGGTVESL
                     NADLLDALLDAVESRAGGPPILLTAPRAGYGKTHLLGRVVAAADRQALMVPMAFQSGD
                     KPGLAMLTRRGLEALARAPAGEREGWTRLRET"
     gene            complement(682..1278)
                     /gene="tsf"
                     /locus_tag="PLP58_16255"
     CDS             complement(682..1278)
                     /gene="tsf"
                     /locus_tag="PLP58_16255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007418359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor Ts"
                     /protein_id="HRJ10133.1"
                     /translation="MAEITAKLVQTLREKTNAGMMECKAALKEADGDLEKAETILRKK
                     GTIKAEKKADRQTKEGIIASYIHMAGRIGVLIEVNCETDFVARNENFQALVKDISLHI
                     AAASPKYVGREEVPADLVAKEREIGAEQVKGKPANIVEKIVDGKMDKFYADHCLLEQA
                     FIKNPDINIGDLIKSKVAELGENLVVRRFTRYAVGEEV"
     gene            complement(1310..1996)
                     /gene="rpsB"
                     /locus_tag="PLP58_16260"
     CDS             complement(1310..1996)
                     /gene="rpsB"
                     /locus_tag="PLP58_16260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009958899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HRJ10134.1"
                     /translation="MSQALAELVEAGVHFGHQTRRWNPKMKPFILESRNQIHILNIEE
                     TLTQIATAAEFLAGLARKNKRILFVGCKRQAQEAIREAAEACGQFYVNHRWLGGTLTN
                     LETIRKSVARLNYLEEIEKKPEFKMMSKKELAGLNRERIKLERNLRGVRGMEKFPDAV
                     VIVDSAREHIAVHEARRLGIPIVALVDTNADPNIIDYPIAANDDAIRSIRIILQKLID
                     PVIAAMAESK"
     gene            2279..3700
                     /locus_tag="PLP58_16265"
     CDS             2279..3700
                     /locus_tag="PLP58_16265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009962273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HRJ10135.1"
                     /translation="MKPASSFSRRHFLSAALAGGAASQISAQDAQALAKDGQFQEPAR
                     GIPMADDADVIVCGAGPAGVTAAITAARAGARVRLFEWRGCLGGIWTAGLLGYFLDFN
                     KPGFAKELRDKLDARDARANSTSTSRFCYDPEALKLLLEELCLEAGVKFQYHTKVSAA
                     FREGARLTTIVTESKSGRQAWKAPVFIDTTGDGDLGHQAGCAFEIGISEDCPCQPMSL
                     NALLVVKDAEALREFIRFGQPNPGENSDSEKKQRIKDALVSTGHYPSYAGPTMWHVRD
                     NLVFAMMNHEYGVKAWDAAEITAATVRARAEMNKMVAGLRALGGPWEGTQIVATAEQI
                     GVRDGRRIRGRYVVLQDDLANGARHDDAVTRVTFGIDVHALSADDNKKHAIMPKPVKM
                     KPYDIPLRALIAKDVDGLMMAGRCISGDFIAHSSYRVTGNAVAMGEAAGVTAALAALS
                     KRLPHEVAWSEGEARLREMGQRV"
     gene            complement(3719..4897)
                     /locus_tag="PLP58_16270"
     CDS             complement(3719..4897)
                     /locus_tag="PLP58_16270"
                     /inference="COORDINATES: protein motif:HMM:NF025056.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIR domain-containing protein"
                     /protein_id="HRJ10136.1"
                     /translation="MIAFSYRREDSQAAVGRLADKLTAEFGKEAVFLDQDSIPLGVDF
                     RSHVREVWARSRVVLVVIGERWFGSRPDGRRRLDESADHVRVEVEQAMTASALVIPIL
                     LDQTAMPKVEELPESLSDLPFLNALRLDTGRDFHHHADRLIRELKRLLAEPARKQTSA
                     PSPSPGTDAQAAGSARQRSSALQSLGTRLAGIFATPAHPEPAPAATQTRKPDEAPPPV
                     RMPPERPVVLPQVPAPPDLPSAIQPQTHLNLLPAKPKEPTATDVQQPQKGRFVQEEPT
                     VVHGEDLDVPTFLRKKKPASPPSKADGGEGKLGRQAVFMINGDLVISGIDLPGGKIRF
                     RHKNGGLVQADGTDWRAEIEEDGRLLRLIRNGLPEGFARLSSMDSVLLKRLLRRAASQ
                     "
     gene            5056..7527
                     /locus_tag="PLP58_16275"
     CDS             5056..7527
                     /locus_tag="PLP58_16275"
                     /inference="COORDINATES: protein motif:HMM:TIGR02604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ10137.1"
                     /translation="MSRLPAPLLLLSASLLHAAPPPKAPDVPAEVPPKIETLQPGVKM
                     TLLAEHPDLVTPTGIDVDDQGRVWLVACHTHFRPEGYTGPGHDEVLVFDAEGKNRRVF
                     YNATTATMNLQLGPDGWVYLAERSRILRVKDTDGDGKGDLEETLAVLDTLADYPHNGL
                     SGMAWDPQGGLVFSLGENFGKDWTLTGTDGAQVSGRGEGGVFRCAPDGKALRRIARGF
                     WNPFGLLVRADGEIFAAENDPGSRPPCRLLHIVEGADYGYQWVYGSAPVHPFVAWNGE
                     LRGTLGMVHPCGEGPCAILDLGGGLIIPSWSDHRIDYYPLTRKGAGHTSERVPLVKGS
                     DYFRPTCMARGPDGAFYLTDWVFSSYPIHQRGRLWKLEMDPQAATWIKAAPDPLNEAA
                     RLAHDLRTGKATLPFARLLALAQGDDTCLADAALTALARASTGWTPETLRAMSAPDRL
                     WSFIALRRKDITDEQWPRAFLRDTDPELRFEALRWIADAVLTPFLSEVEAMLSDTTLD
                     FRLFEAALAAWNTLRGEPGAGVTNPGVLAARITDPRTPARIKSYALRLAPPDHKLITV
                     PLLQELLDSGDDDLALEAARTLAARNTDDARLVLAKTAADEKLHPALRAEAIAGLAAG
                     TQPGLHALLAKIAEKSPGIVRQEARRALRFTEHDTTFTPDAGRPAFDATTAWLQRLDA
                     LPGQPDPEAGRRLFFHTKVALCASCHRHSGRGTVLGPDLTLIARQGGREDILRSILEP
                     HREVAPQFYPSQVRLKDGTEFTGILLRSSSTEVFRDLAGKERSFPKDAIHSRTELKTS
                     LMPPGLVLSLTDTELRDLLAFLHPR"
     gene            complement(7611..8615)
                     /locus_tag="PLP58_16280"
     CDS             complement(7611..8615)
                     /locus_tag="PLP58_16280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ10138.1"
                     /translation="MKSTSLLLAALASTLLLSQCVLPATARNNGAKVNLPYAWTLEYG
                     ETMEWTGGKTEKGFANGKGKLFLYAVDGKKRLEMDTECAAGKFHGPYVWRSFKDGIVY
                     AEVVGTYSKGIQVGEFGSRYFQADTPDSYVMKKGSYNGYGQLHGQCEQRTLDGTRYVS
                     QWSNGSHLSKTIYNPDGSVKQPVAAASSSSSYSDTDILGGMFALGGLAGGDANLLMGG
                     VSMMAGDDAGAIQHIANMGGGGGSTGGLTGAAAGQAPKKIVANKRSLFDLPSHSHLAR
                     FRSAEGDHIKFYIDSADRAYADYKRTGEEQYYTRHREYADTALQFHKQTSTQGTRMIR
                     "
     gene            complement(9131..10495)
                     /locus_tag="PLP58_16285"
     CDS             complement(9131..10495)
                     /locus_tag="PLP58_16285"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arylsulfatase"
                     /protein_id="HRJ10139.1"
                     /translation="MKCPRALLFLALAVCPLLAAAPPNVIYILADDLGYGDLGSYGQK
                     LIRTPRLDRMAEQGMRFTQHYSGAPSCHPSRCALFTGLHTGHGRIRENCKTPLPEEDF
                     TLSQMFQQAGYATGGIGKWALGDSGTTGAPWRKGMDEFLGYLDQTHAHGYYPEYLWND
                     GSRMDLPENADGRRGTYSHDLFAGRALDFIRRHKDKPFFFYAAFTIPHAEVAVPEDSL
                     AEYRGKWEEPKKFEGSKTYCPQDQPRAVRAAMITRLDHDVGRILDLLDELGLSENTLV
                     IFTSDNGPITAGGQDPVFFNSAGPLRGMKFGLYEGGIRVPCLARWPGKVKAGSVSDRV
                     SDFTDMFPTFAELIGAPAPEGLDGVSILPTLLGRPEQQKPRQLHYWEAAPQQALRMGD
                     WKAYRPAPDKPLELYNLAGDIGEKNDLAAQLPERAAEMERLMAENRTDHPDFPLAKKP
                     KKGK"
     gene            10671..>10954
                     /locus_tag="PLP58_16290"
     CDS             10671..>10954
                     /locus_tag="PLP58_16290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ10140.1"
                     /translation="MKNPKLLLRADATAAMGTGHVMRCLALAQAWQDMGGTVGLLARD
                     LPAALAARCGEEGVTVLPFDPVAPDGEQTAAAARAADWTVLDGYHFDAAFG"
BASE COUNT         1750 a         3835 c         3403 g         1966 t
ORIGIN      
        1 gtctcgcgca gccgcgtcca gccttcgcgt tcgcctgccg gcgcgcgcgc cagcgcctca
       61 aggccgcgcc gggtcagcat ggccaggccg ggcttgtcac cgctttgaaa cgccatcggc
      121 accatcagag cctggcggtc cgccgcggcg acgaccctgc cgagaagatg cgtcttgcca
      181 tatccagcgc gcggggctgt gagcaggatc ggcgggccgc ctgcgcgcga ctccaccgca
      241 tccagcagcg cgtctagcaa gtccgcgttc aggctttcca cagtgcctcc ggcctccaaa
      301 tcaaacggcc ctgtgcagac atccgcgctg aaaggattac ctccggcgga ggatcgtgga
      361 tgggcagcgc ctgagttcac tggggtctgg actttcgtca cgggagggga tgtttaatct
      421 ttgtgaaatt atggctaact attgaaacta ttctatgcca tgaagtttta atgcaagcgg
      481 ttcatccatt tgtgaataat ttcaatctcg caaaagtgat acaagcgggt gtgcgagggg
      541 agtttgtcgg tcgctgatcc gggcagacaa gattcgcctg tcccggcgac ttccccgcct
      601 ggagcgtgac cccatgaaaa agggcggacc gaagtccgcc cttgagttga ttcatcagtt
      661 ctctgcaatg aggcgtctcg atcaaacttc ctctcccacc gcgtagcgtg tgaagcggcg
      721 cacgacgagg ttttcgccga gttcggccac cttgcttttg atcaggtcgc cgatgttgat
      781 gtcggggttc ttgatgaagg cctgctccag gaggcagtgg tcggcgtaaa atttgtccat
      841 tttgccgtcc acgatcttct cgacgatgtt cgcgggcttg cctttgacct gttcggcgcc
      901 gatttcgcgc tctttggcga cgaggtcggc gggcacttcc tcgcggccca catatttcgg
      961 ggaggcggcg gcgatgtgca gggagatgtc cttcaccagc gcctggaagt tttcattgcg
     1021 ggcgacgaag tcggtctcgc agttcacttc gatgagcacg ccgatgcggc cggccatgtg
     1081 gatgtaggag gcgatgatgc cttccttggt ttggcggtcg gccttcttct cggctttgat
     1141 ggtgcctttt ttgcgcagga tggtctcggc cttctccagg tcgccgtcgg cttccttgag
     1201 ggcggccttg cactccatca tgccggcgtt ggttttttca cggagggtct ggacgagttt
     1261 ggcggtgatt tctgccatgg taagtcgggg ttcggggaaa gggttgaggt tacttggatt
     1321 cggccatcgc cgcgatcacc gggtcaatca gcttctgcag gatgatgcgg atggagcgga
     1381 tggcgtcgtc attggcggcg atggggtagt caatgatgtt cgggtcggcg ttggtgtcca
     1441 cgagggccac gatcgggatg cccagacggc gggcctcatg cacggcgatg tgctcgcggg
     1501 cggagtccac gatgacgacc gcgtcgggga acttttccat gccgcgcacg ccgcgcaggt
     1561 tgcgctccag cttgatgcgc tcgcggttca ggccggcgag ctccttcttc gacatcatct
     1621 tgaactcggg cttcttctcg atctcctcaa gataattcag gcgggccacg ctcttgcgga
     1681 tggtctccag gttggtgagc gtgccgccca gccagcggtg gttgacataa aactggccgc
     1741 aggcctcggc ggcctcgcgg atggcctcct gggcctggcg tttgcagccg acgaaaagga
     1801 tgcgcttgtt tttgcgggcc agtccggcca ggaattcggc ggcggtggcg atctgggtga
     1861 gcgtctcctc gatgttgagg atgtggatct ggttccggga ttccaggatg aaaggcttca
     1921 tcttggggtt ccagcggcgg gtttgatggc cgaaatggac tccggcttcg acgagttctg
     1981 cgagggcttg tgacatgtgg attgggtgcc gtctcctgca cgcggattcc ggcggggccg
     2041 gcgcgctcag cgcatggggc gctgatccag aaggtggggc gtttgtggtt tgaatttccc
     2101 atccgccggg caccatgccg ggcaaacggg ccgggatagt aggagacatg gccgggacgg
     2161 caagcggttt ttcgcgacgt ttccgggcgc gtttttccca cagctttgag gggcctcccc
     2221 ggcttcatgc cgcgattcct tgaaatgccc cgccttcctg cttcgtgctc tttcctccat
     2281 gaaacccgcc tcctccttct cccgccgcca cttcctctcc gccgcgctcg ccggcggtgc
     2341 tgccagccag atttccgcgc aggacgcgca ggctctggcg aaagacggcc agttccagga
     2401 acccgcgcgc ggcatcccga tggcggacga cgccgatgtc attgtctgcg gcgccggacc
     2461 cgcgggtgtc accgccgcca tcaccgccgc gcgcgccgga gccagggtgc gcctctttga
     2521 atggcgcggc tgcctcggcg gcatctggac cgcgggtctg ctgggttact tcctcgactt
     2581 caacaagccc ggcttcgcca aagagctgcg cgacaagctc gacgcccgcg acgcgcgcgc
     2641 caactccacc agcaccagcc gcttctgcta cgacccggag gcgctcaagc tgctgctgga
     2701 ggagctttgc ctggaggcgg gcgtgaagtt ccaataccac accaaagttt ccgccgcctt
     2761 tcgcgagggc gcgcgcctca ccaccatcgt caccgagtcc aaatccggcc gccaggcatg
     2821 gaaagccccc gtcttcattg acaccaccgg cgacggcgac ctcggccacc aggcgggctg
     2881 cgcctttgag atcggcatct ccgaggactg cccctgccag cccatgtccc tgaacgcgct
     2941 cctcgtcgtc aaggacgcgg aagcgctgcg cgagttcatc cgcttcggcc agcccaatcc
     3001 gggggagaac tccgacagcg agaaaaagca gcgcatcaaa gacgctctcg tctccaccgg
     3061 ccactacccc tcctacgccg gcccgacgat gtggcatgtg cgcgacaacc tcgtcttcgc
     3121 catgatgaac cacgagtatg gcgtcaaagc ctgggacgcc gccgagatca ccgccgccac
     3181 cgtgcgcgcc cgcgccgaga tgaacaaaat ggttgccggc ctgcgcgccc ttggcggccc
     3241 ctgggagggc acccagatcg tcgccaccgc cgagcagatc ggcgtgcgtg acggacgccg
     3301 catccgcggc cgctacgtcg tgctccagga cgacctcgcc aatggcgcgc gccatgacga
     3361 cgcggtcacg cgcgtcacct tcggcattga tgtccacgcc ctcagcgccg acgacaacaa
     3421 gaagcacgcc atcatgccca agcccgtcaa gatgaagccc tatgacatcc ctctgcgcgc
     3481 tctcatcgcc aaagacgtgg acggactcat gatggcgggc cgctgcatca gcggcgactt
     3541 catcgcccac tccagctacc gcgtcaccgg caacgccgtg gcgatgggcg aagccgccgg
     3601 cgtcaccgcc gcgctcgccg ctttgtccaa gcgcctccct cacgaggtcg cctggagcga
     3661 gggcgaggcc cgcctgcggg agatgggcca gcgggtgtga ctgcgccgcc tgcgtggatc
     3721 actggctggc ggcacgcctt agcaggcgct tcaaaaggac ggaatccatg ctgctcaagc
     3781 gggcgaatcc ctccggcagg ccatttcgga tcagccgtag aagccgcccg tcttcttcga
     3841 tctccgcccg ccaatccgtg ccatcggctt gcacaaggcc gccgtttttg tggcgaaagc
     3901 ggatcttgcc tcccggcagg tcaatgccgc tgatgaccag atcaccattg atcatgaaca
     3961 cagcctggcg gcccagcttt ccctcgccgc cgtccgcctt cgatggcggg gatgcaggct
     4021 tctttttccg caaaaacgtc ggcacatcca gatcctcccc gtgaacgact gtcggctcct
     4081 cttgaacaaa gcggcccttc tgcggctgct ggacatccgt tgcagttggt tccttgggct
     4141 tggctggaag cagattcaga tgtgtttgcg gctggattgc ggaaggcaaa tccggcggcg
     4201 ccggcacttg aggcagaacc accggcctct caggcggcat ccgcactgga ggcggcgctt
     4261 cgtcgggctt cctcgtttga gtggctgctg gcgccggctc tggatgggca ggcgttgcga
     4321 agatgcccgc caggcgtgtt ccgagagact gcaacgccga ggatcgctgc cgcgcgctgc
     4381 cggcggcctg ggcgtcagtt ccgggagacg gtgacggggc ggacgtttgc ttccttgcag
     4441 gctccgcgag caggcgcttc agctcccgga tcaggcggtc cgcgtgatga tgaaagtccc
     4501 gcccggtgtc caggcgcagg gcattgagga aaggcaggtc ggacagcgac tccggcagct
     4561 cctccacctt cggcatcgct gtctggtcca gcaggatcgg gatcaccaga gcgcttgccg
     4621 tcatggcctg ctccacctcc acgcgcacat gatccgcgct ttcatccagc ctgcggcgtc
     4681 cgtccggccg tgagccgaac caccgctcgc caatcaccac cagcaccacg cgcgaccttg
     4741 cccacacctc cctcacatgg cttctgaaat ccacacccag cgggatcgag tcctggtcca
     4801 gaaacaccgc ctctttgcca aattccgccg tcagtttgtc cgccagcctg cctacagcag
     4861 cctggctgtc ttccctgcgg taggagaaag cgatcatgac gacggcgcaa gcatagccag
     4921 ccctcatccc ggcaggcaag cactctttga ccaggcgcgc aggaatgccg gccctgccag
     4981 cgcgagccgc cctgtcatga ctgccccgcg catttcccgc cagcgcgggt ctccgggccg
     5041 cgtagtctgt gccccatgtc ccgactgccc gcccccctgc tcctgctctc cgcatccctc
     5101 ctccacgccg ccccgccgcc gaaagcgccc gacgtgcccg ccgaggtgcc gccgaagatc
     5161 gagaccctcc agcccggtgt gaaaatgacc ctgctcgccg agcacccgga cctcgtcaca
     5221 cccaccggca ttgatgtgga cgaccagggc cgcgtctggc tcgtggcctg ccacacccac
     5281 ttccgcccgg agggctacac cggtcccggg cacgacgagg tgctcgtctt tgacgccgaa
     5341 ggcaaaaacc gccgcgtctt ttacaacgcc accaccgcca ccatgaatct ccagctcggc
     5401 cccgatggct gggtctatct cgccgagcgc tcccgcatcc tgcgcgtgaa ggacaccgac
     5461 ggcgacggca aaggcgacct ggaggaaacc ctcgccgtgc tcgacaccct ggccgactac
     5521 ccgcacaacg gcctcagcgg catggcctgg gacccgcagg gcgggctcgt cttttccctc
     5581 ggcgaaaact tcggcaagga ctggaccctc accggcaccg atggcgcgca ggtcagcgga
     5641 cgcggcgagg gcggcgtctt ccgctgcgcg ccggatggca aagctctgcg ccgcatcgcg
     5701 cgcggtttct ggaatccctt cggcctgctc gtgcgcgccg atggtgaaat cttcgccgcc
     5761 gagaacgacc ctggcagccg cccgccctgc cgcctgctcc acatcgtcga aggcgccgac
     5821 tacggctacc agtgggtcta tggcagcgcc cccgtgcatc cctttgtcgc ctggaatggc
     5881 gagctgcgcg gcaccctcgg catggtccac ccctgcggcg agggcccctg cgccatcctc
     5941 gacctcggcg gcggactcat catcccgagc tggagcgacc accgcattga ctactacccg
     6001 ctcacccgca aaggcgcggg ccacacctcc gagcgcgtgc cgctggtgaa aggcagcgac
     6061 tacttccgcc ccacctgcat ggctcgcggg ccggacggcg ccttttacct caccgactgg
     6121 gtcttcagct cctaccccat ccaccagcgc ggccgcctct ggaagctgga gatggatccg
     6181 caggccgcca cctggatcaa agccgcgccc gatcctctga acgaagccgc ccgcctcgcc
     6241 catgacctcc gcaccggcaa agccaccctg cctttcgcga ggctgctcgc ccttgcccag
     6301 ggcgatgata cctgcctcgc cgatgccgcc ctcaccgccc tggcccgcgc cagcaccggc
     6361 tggacacccg aaaccctgcg cgccatgtcc gcgccagacc gcctctggtc cttcatcgcc
     6421 ctccggcgca aggacatcac cgacgagcaa tggccccgcg ccttccttcg cgacaccgac
     6481 cccgaactgc gcttcgaggc cctgcgctgg atcgccgacg ccgtgctcac cccctttctc
     6541 tccgaggtcg aggccatgct ctcggacacc acgctcgact tccgcctctt cgaagccgcc
     6601 ctcgccgcct ggaacaccct gcgcggcgag cccggcgccg gcgtcaccaa ccccggcgtc
     6661 ctcgccgccc gcatcaccga cccgcgcaca cccgcccgca tcaaaagcta cgctctccgc
     6721 ctcgccccgc ccgaccacaa gctcatcacc gtccctctcc ttcaggagtt gctcgactcc
     6781 ggtgatgacg atctcgccct cgaagccgcc cgcaccctcg ccgcgcgcaa caccgacgac
     6841 gcccgcctcg tcctggcaaa gaccgccgcc gatgaaaaac tccaccccgc cctgcgcgcc
     6901 gaagccatcg ccggactcgc cgcaggcacc cagcccgggc ttcacgccct gctggcaaag
     6961 atcgccgaaa aaagccccgg catcgtgcgc caggaggccc gccgcgccct ccgcttcacc
     7021 gagcacgaca ccaccttcac cccggacgcc ggccgccccg ccttcgatgc caccaccgcc
     7081 tggttgcaaa ggctcgacgc cctccccggc cagcccgacc ccgaagccgg acgccgcctc
     7141 ttcttccaca ccaaagtcgc cctctgcgcc tcctgccacc gccacagcgg gcgcggcacc
     7201 gtcctcggcc ccgacctcac cctcatcgcc cgccagggcg gccgcgagga catcctccgc
     7261 tccatcctcg aaccccaccg cgaggtcgcc ccgcagttct atccctcgca ggtgaggctc
     7321 aaggacggca ccgaattcac cggcatcctc ctgcgctcct ccagcactga agtcttccgc
     7381 gacctcgccg gcaaggagcg cagcttcccc aaagacgcca tccactcccg caccgagtta
     7441 aagacctccc tcatgccccc cggcctcgtc ctctccctca ccgacaccga actgcgcgac
     7501 ctgctcgcct tcctccaccc gcgctgaccc cgcgtgcaga gcggcggagc tcacgccggc
     7561 agcgccttcg cctgttgcgg cccgcacggc gcgtcgaaga gccgcagcgc tcagcggatc
     7621 atccgggtgc cctgggtgga ggtctgtttg tggaattgca gtgccgtgtc cgcatactcc
     7681 cggtgccggg tgtaatactg ctcctccccg gtcctcttgt aatccgcata agcccggtcc
     7741 gccgagtcaa tgtagaactt gatgtggtcc ccctccgccg agcggaagcg cgcgagatgg
     7801 ctgtgggaag gcaggtcaaa gaggctgcgc ttgttcgcca cgatcttttt cggagcctgg
     7861 cccgccgccg cgccggtcag gccgcctgtg gagccgccgc cgccgcccat attggcaatg
     7921 tgctgtatcg ccccggcgtc atcccccgcc atcatggaga caccgcccat caaaagattc
     7981 gcgtcaccgc ccgccagtcc gcccagggcg aacatgccgc ccaggatgtc cgtgtccgag
     8041 taggagctgg acgaggaggc cgccgccacg ggctgcttca cgctgccgtc ggggttgtaa
     8101 atcgtcttcg agagatggct gccgttgctc cattgggaca cataccgcgt tccgtccagc
     8161 gtccgctgct cgcattggcc gtgaagctgg ccgtagccgt tgtaactgcc ctttttcatc
     8221 acataactgt caggagtgtc cgcttggaaa tagcgggatc caaactctcc gacttgaatg
     8281 cctttggaat aggttccaac aacctcagca tagactattc catccttgaa actacgccac
     8341 acatacggcc cgtggaattt gccggcggcg cattcggtgt ccatttccag ccgctttttg
     8401 ccatccacgg cataaaggaa gagtttgccc ttgccgttgg cgaagccttt ctccgtcttc
     8461 ccgcccgtcc actccatcgt ctcgccgtat tccagcgtcc aagcataagg caggttcact
     8521 ttcgcgccgt tgttgcgcgc cgtcgcgggc aggacgcatt ggctgagcag cagggtggag
     8581 gccagggcgg ccaggagcag gcttgtggat ttcatgggag cgggagcagg ttggaggtga
     8641 gagggagccg gcctgcgcag gccgggattc ccagctttct gcgccatccg cgcgcggatg
     8701 taacgaaaaa aaacgcgccc ggtaaaaaat ccccgcgccc ggcatttgtc atcaggcaca
     8761 gcccgcagcc gcctcagtcc ccgtccccgg aacggcacat cagccggatg gcgttgacga
     8821 gcaggagcgc gccggcgcat aacgccagga acgtgctgcc tgtcatcgcg gcggtaataa
     8881 tgatgaccgg cagcagcagc acaggcaggc cgatggggcc aggctgtcgg tggcgtcatt
     8941 ccctttcatg gccgcaccct tcgtgacctt gcctgccgcg tcaaccgccg ccctgcatgc
     9001 ccggccagac accagttttc aggaaccatg ccagacacaa gcccagcgtc accgccctcg
     9061 cgcggtgaga gcgcgtgagt cattcatgag actcgaagcg gaagaggtgt gtgcccgcgc
     9121 tgctggcgtg tcacttgccc tttttcggct tcttggcgag cgggaagtcc gggtggtcgg
     9181 tgcggttctc cgccatgagc ctctccattt cggcggcgcg ctcgggcaac tgggcggcga
     9241 ggtcgttctt ctcgccgatg tccccggcca ggttgtaaag ctccagcggc ttgtccggcg
     9301 cggggcggta ggctttccag tcgcccatgc gcagggcctg ctgcggggcg gcctcccagt
     9361 agtgcagttg gcgcggcttt tgctgctcgg gccggcccag cagcgtgggc aggatggaga
     9421 cgccgtcgag cccctccggc gcgggcgcgc cgatcagctc ggcgaaggtg ggaaacatgt
     9481 ccgtgaagtc gctgacgcgg tcgctgacac tgccggcctt cactttgccc ggccagcgcg
     9541 cgaggcaggg cacgcggatg ccgccttcat aaaggccgaa cttcatgccg cgcagcgggc
     9601 cggcgctgtt gaagaacacg gggtcctgcc cgccggcggt gatggggccg ttgtcgctgg
     9661 tgaagatgac cagcgtgttc tccgagaggc ccagctcgtc cagcaggtcc aggatgcggc
     9721 ccacgtcgtg gtccaggcgc gtgatcatgg cggcgcggac ggcgcgcggc tggtcctgcg
     9781 ggcagtaggt cttgctgccc tcgaacttct tcggctcctc ccatttgccc cggtattcgg
     9841 ccagcgagtc ctccggcacc gccacctccg cgtgcgggat ggtgaaggcg gcgtagaaaa
     9901 agaagggctt gtccttgtgc cggcggatga agtccagcgc gcgcccggcg aagaggtcgt
     9961 gcgagtaggt gccgcgcctg ccgtccgcgt tctccggcag gtccatgcgg ctgccgtcat
    10021 tccagagata ctccgggtag taaccgtgcg cgtgggtctg gtccaggtag ccgaggaatt
    10081 cgtccatgcc cttgcgccag ggagcgccgg tggtgccgga gtcccccagc gcccacttgc
    10141 cgatgccgcc ggtggcgtaa cccgcctgct ggaacatctg cgagagcgtg aagtcctcct
    10201 ccggcagcgg cgtcttgcag ttctcccgga tgcgcccgtg cccggtgtgc agtccggtga
    10261 agagcgcgca gcgggagggg tggcaggagg gcgcgccgga gtaatgctgg gtgaagcgca
    10321 tgccctgctc cgccatcctg tccagacgcg gggtgcggat cagcttctgc ccgtagctgc
    10381 cgaggtcccc gtagcctagg tcatccgcca ggatgtagat gacgttcggc ggggcggcag
    10441 ccaggagcgg gcagacggcg agtgcgagga aaagcagggc gcgtggacat ttcatgcccc
    10501 ctggcaacgc ggccaccctg gccgcctatc aaaaggccgc cacgtccatg acacccggcg
    10561 ccactccggc acggcggtca atgcgggggc tttctgcggg aaggcaagct tggggttgtc
    10621 cgagaggcga ttccagttca caaccttcat gccacacatc cgccctccct ttgaaaaacc
    10681 ccaagctcct cctccgcgct gacgccaccg ccgccatggg cacggggcat gtgatgcgct
    10741 gcctggcgct ggcgcaggcg tggcaggaca tgggcgggac ggtgggtttg ctggcgcggg
    10801 atttgccggc ggcgctggcg gcgcgctgcg gggaggaggg cgtcacggtt ttgcctttcg
    10861 acccagtggc gccggatggg gagcagacgg cggcggcggc gcgcgcggcg gactggacgg
    10921 tgctggatgg ctaccacttt gacgcggctt tcgg
//
LOCUS       DAOMYJ010000180        10939 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Prosthecobacter sp. isolate AMR_MDS_5629
            NODE_2000_length_10939_cov_21.769938, whole genome shotgun
            sequence.
ACCESSION   DAOMYJ010000180 DAOMYJ010000000
VERSION     DAOMYJ010000180.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867850
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prosthecobacter sp. (wastewater metagenome)
  ORGANISM  Prosthecobacter sp.
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Verrucomicrobiaceae; Prosthecobacter.
REFERENCE   1  (bases 1 to 10939)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10939)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.30
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:34:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,742
            CDSs (total)                      :: 4,684
            Genes (coding)                    :: 4,676
            CDSs (with protein)               :: 4,676
            Genes (RNA)                       :: 58
            tRNAs                             :: 55
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10939
                     /organism="Prosthecobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2000_length_10939_cov_21.769938"
                     /isolate="AMR_MDS_5629"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1965333"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..604
                     /locus_tag="PLP58_16295"
     CDS             <1..604
                     /locus_tag="PLP58_16295"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012420157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HRJ10141.1"
                     /translation="AENAPEARALGTLRETMVFATGSPDAEIMLVGEAPGAEEERLRE
                     PFVGPAGQKLTGILKAMGLERAQVYISNICKFRPSLPNQGGSNRAPTPEEMRACRPFI
                     LTEIEIVRPKVIVALGATAMQGLGLQGAVSSMRGRFHEVGGIPLMVTWHPSYILREER
                     NSGGQAFAAKRQVWEDMLKVMEKCGMAVSARQRGYFQNKG"
     gene            611..1456
                     /locus_tag="PLP58_16300"
     CDS             611..1456
                     /locus_tag="PLP58_16300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009966132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HRJ10142.1"
                     /translation="MLAPFEYDYMVKAILVSGLIGGVCAFLSCFITLKGWSLMGDALS
                     HAIVPGVSLAWLAGLPFSAGAFFAGVLAALGMGWVRRNSRLREDAVIGVVFTTFFAAG
                     LLLLTLYPSNLNLRTIVFGNILAISDPDILQVLVISGLSILVLALKWRDLLLYCFDEA
                     HARSLGLNTGFLHFLLLALLSATAVAALQTVGACLVVAMLVTPGATAYLLTDRFGKML
                     GIATAAGAGCGVAGAYASWFFNGSTGGCIVVLQTLVFLAVLVLAPKHGLLASRRQRRL
                     AASLS"
     gene            1453..2283
                     /locus_tag="PLP58_16305"
     CDS             1453..2283
                     /locus_tag="PLP58_16305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009966130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HRJ10143.1"
                     /translation="MSLLEPFRYPFMTEALLAGALVGAVCALFSCYLVLKGWSLMGDA
                     ISHAVLPGIVLAHMAGLPLALGAFATGLLCAAATGWIKASTRIKEDTVMGIVFTGLFA
                     LGLVLFTKVETDAHLNHILFGNILGIERADMIQTVTASTLTLVVVLLMRRDLMIFCFD
                     PAQARAIGLNTGLLYYVLLSLLAGTVVASLQAVGIILVVAMLVTPGCTARLLTDRFDR
                     MLLLAAGSSVLATCAGVYASFFINGSTGACIVLAQAALFMLAFVFGPKHGLLAARRAA
                     "
     gene            complement(2291..2836)
                     /locus_tag="PLP58_16310"
     CDS             complement(2291..2836)
                     /locus_tag="PLP58_16310"
                     /inference="COORDINATES: protein motif:HMM:NF024822.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4112 domain-containing protein"
                     /protein_id="HRJ10144.1"
                     /translation="MPSEIPDRIIIDQVLPPSAKGMAARAAGESELARYLAKWLDNWL
                     RIPGTDFKIGLDPILALIPGLGSGVASGGGVIILLEAIRSGVTLPVFIRMGGNLLLNT
                     LFDFLPLGGPVASAFFKSNMRNLRLLQAWQAGNQAAVKKSTLRLFLSLGLLLAALAAM
                     IIGLWIFYAWLISRVFQHLLG"
     gene            complement(2895..3941)
                     /locus_tag="PLP58_16315"
     CDS             complement(2895..3941)
                     /locus_tag="PLP58_16315"
                     /EC_number="1.14.19.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009960188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016717 - oxidoreductase activity, acting
                     on paired donors, with oxidation of a pair of donors
                     resulting in the reduction of molecular oxygen to two
                     molecules of water [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid desaturase"
                     /protein_id="HRJ10145.1"
                     /translation="MSSAQPPPAVKPQAGISEWKAIIASYAAPSMPRAVWQIVNTLVP
                     YFALWYLMWLSLSVSYWLTLGIAALAGLLLVRVFIIFHDCCHGSFFKSKPANDILGFF
                     TGMLTFTPYGHWRWQHNVHHATSGDLDRRGEGDIWTLTVREYLEAPRWKKIAYRLARN
                     PFVLFVIAPLGLFWIYQRFPSSGAKGKDRASVMWMNLGVTAMVAAGCWLFGWQAYLMM
                     QLPVTLVAGAAGIWMFYVQHQYEDTYWENRENWDYTTAALEGSSFYKLPKVLQWFTGN
                     IGYHHIHHLSSRIPNYNLERCHHSHPLFQAVKPVTLLGSLKCASLRLWDEQSRKLVGY
                     AHLKKLRGQNQAAA"
     gene            complement(4153..4281)
                     /locus_tag="PLP58_16320"
     CDS             complement(4153..4281)
                     /locus_tag="PLP58_16320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ10146.1"
                     /translation="MTTTPVTCPSCFQEFEVPAPWPDETPCDVDYDCEVCCRPMRI"
     gene            4616..5230
                     /locus_tag="PLP58_16325"
     CDS             4616..5230
                     /locus_tag="PLP58_16325"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF014796.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HRJ10147.1"
                     /translation="MKETLDAQSISVGNVAALMSNIEGIIRPSNSRSVLPLGRLISLG
                     MSGSSDHVLPPKTVRVIRQSGGGHVNHTAFWRFLGPHGSGPSGPQGDSARAIREHFGS
                     VRNFRRFFKEVAMSHVGSGWAWLVFRQDKALVVTTTFNEDNPLMKDIIPWHQAGRPIL
                     ALDLWEHSYYDAHGDDRESYVDSWWKVVNWTFVERAYQIVTKIS"
     gene            5458..5865
                     /locus_tag="PLP58_16330"
     CDS             5458..5865
                     /locus_tag="PLP58_16330"
                     /inference="COORDINATES: protein motif:HMM:NF018942.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0030416 - methylamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MauE/DoxX family redox-associated membrane
                     protein"
                     /protein_id="HRJ10148.1"
                     /translation="MKLLRFLLHLAFGGVFVYAGALKARDPAAFVMDVRSFELLPDPW
                     AAWLAMCLPWLEIFAGLAVITGVLRAGGLLVLNSSLLAFLAAILSAWARGLDIQCGCF
                     GGGAGTSSHVELIARDVALLALGMWLACSRGKG"
     gene            5906..7402
                     /locus_tag="PLP58_16335"
     CDS             5906..7402
                     /locus_tag="PLP58_16335"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arylsulfatase"
                     /protein_id="HRJ10149.1"
                     /translation="MKLVPLVLSCLGLSAASAAEKLNVVFILADDLGWGEVGCYGQKK
                     IPTPNLDRMAAEGMRFTQHYSGAPVCAPSRCVLMTGKHLGRAEVRGNLSMPKVDPKYD
                     EGQWPISENAFTLAMAFQKAGYATGAMGKWGLGPVGSTGEPNNKGFDLFFGYNCQGVA
                     HSFYPPHIWRNTEKIIINKNPIPGHAKQPEGEIKLEDWIGETYAPKLMIAEAEKFIES
                     SCARPFFLYLPFIEPHVSMHPPKESVDKFPAEWDDEAYRGQCGYVPHPRPRAGYAAMI
                     SDLDGYVGRVMKKLEETGVADRTLVIFSSDNGTTHPRLPATHFHVGGVDGAFFNSTAG
                     LRGWKGSVYEGGLRVPTLAKLPGVIPAGRVNESPGYFADWFPTLCEAAGLEKPDGLDG
                     ESLWPVLTGQVAKQARSRPMIWVFPEYQGQVAIRLGGMKLVRQNLKTKAPGPWEVYDL
                     DKDPGESNDLSASHSALIREAEEVLRREVNDNERFPLDIPGVTTAAAK"
     gene            complement(7530..8363)
                     /locus_tag="PLP58_16340"
     CDS             complement(7530..8363)
                     /locus_tag="PLP58_16340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ10150.1"
                     /translation="MSRLRFLILWLPALAVALSSLSLPAQVPALQRRQWTVDGVIREA
                     LVHVPANAREKPCPLVFGFHGHGGSMANAARSYRIHSLWPEAAVVYPQGLNTPGQLTD
                     PEGKKPGWQHSIGELGDRDLKFFDAMLATMKTELKVDTRRIYSTGHSNGGGFTYMLWA
                     ARGEVFAAMAPSASAAGRVRGLLKPKPVLHIAGENDELVKYSWQQAMIASLLALNQCG
                     ESGPWELDDRISIHPSKVGCPVLTAIHPGTHKYPAEAPEVIVKFFKSQPAEPARENEG
                     R"
     gene            complement(8493..9572)
                     /locus_tag="PLP58_16345"
     CDS             complement(8493..9572)
                     /locus_tag="PLP58_16345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009960260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M42 family metallopeptidase"
                     /protein_id="HRJ10151.1"
                     /translation="MSPASKTFLLDLLSTPSPTGFEFRGQRKWAEHLRGHADRVESDA
                     YGSAWATLDGGGKKPRRVMFEAHADEIGFMVKHITKEGFIHVDRVGGSDVATARGRRV
                     DILGDKGVVRGIIGNIAIHIRENRDDEKVPKVHELWMDIGAASDKEVASAGIRVGHPA
                     VYSDTVEELGAHRLVGRALDNRVGGFIISEVVSRLAQKKTRPAATVVALNAVQEEIGG
                     HGAKMAAYRLMPDVAIVLDVTHATDTPSVDVKKHGEVKLGGGPSLTHGGANHIEVVRR
                     LIEVAEKRKIPIQHESSSRFTGTDTDVIFHQQHGIPSALVSLPLRYMHSVVEMADLRD
                     IEQVIQLLTAFAESVTDRDEFRVVI"
     gene            complement(9611..>10939)
                     /locus_tag="PLP58_16350"
     CDS             complement(9611..>10939)
                     /locus_tag="PLP58_16350"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit N"
                     /protein_id="HRJ10152.1"
                     /translation="LFFFADKSAAALPAFVQPFHQLDTLAVFYKGFALVITLAVLWLT
                     VESRAYLERYTPGGRHGEMFSLPLIVCAGMMWMASAKDLITVFVCLELVTVSFYVLVA
                     FARKSSFALEAGVKYLILGALSTGVLVYGIAWTYGVTDSMSFEGIAEALAAPAAAKNA
                     ALLGAAFLLAGLGFKVAAAPFHIWVPDVYQGAPNPVTAFLSVGSKAAGFIVLTRVVDA
                     FLAKGSVIASEVQALLLIGGALTVLLGSLPAIFQTNLKRLLAYSSISHAGYLLLALGC
                     SQTARFDLGGSSVVAFYLGTYLPMTVLGFLILTVVRQAGLGDDAQDFAGLAKKNPFLA
                     FAATLGFASLAGIPLTAGFMGKLFVFLGLVENARWFALSCAVIGAAAGFYYYLRPVLV
                     MFTGESGPALKIAAPTRAVLVLLAGAILVIGVFPKPLQGVLKVPVASAAK"
BASE COUNT         1954 a         3476 c         3537 g         1972 t
ORIGIN      
        1 ggcggagaac gcgcccgagg cgcgcgcgct gggcacgctg cgggagacga tggtctttgc
       61 caccgggagc ccggacgcgg agatcatgct cgtcggggag gcgccggggg cggaggagga
      121 gcggctgcgg gagcccttcg tcggccccgc cggccagaag ctgaccggca tcctgaaggc
      181 gatggggctg gagcgcgcgc aggtctatat cagcaacatc tgcaaattcc gcccgtccct
      241 ccccaaccag ggcggctcga accgcgcgcc cacgccggag gagatgcggg cctgccggcc
      301 cttcatcctc accgagatcg agatcgtccg cccgaaggtc atcgtggcgc tgggggccac
      361 ggcgatgcag ggcctggggc tgcagggcgc ggtcagctcc atgcgcgggc gcttccacga
      421 ggtgggcggc attccgctca tggtgacctg gcacccgagc tacatcctgc gggaggagcg
      481 gaactccggc ggccaggcct tcgcggccaa gcgccaggtg tgggaggaca tgctcaaggt
      541 catggaaaag tgcggcatgg ccgtgagcgc caggcagcgc ggctacttcc aaaacaaagg
      601 ctgacccgtg atgctcgcgc cttttgagta cgattacatg gtgaaggcca tcctggtcag
      661 cggcctcatc ggcggcgtgt gcgcgttttt gtcctgcttc atcacgctga agggctggtc
      721 gctgatgggt gacgcgctct cccacgccat cgtgccgggc gtctcgctgg cctggctggc
      781 ggggctgccc ttttccgcgg gggccttctt cgccggggtg ctggcggcgc tgggcatggg
      841 ctgggtgcgg cggaactcgc ggctgcggga ggacgcggtc atcggcgtgg tcttcaccac
      901 cttcttcgcc gccgggctgc tgctgctgac gctttacccg agcaacctga acctgcgcac
      961 catcgtgttc gggaacatcc tcgccatctc cgaccccgac atcctgcagg tgctggtcat
     1021 ctccggcctg tccatcctcg tgctggcgct caagtggcgg gacctgctgc tctactgctt
     1081 cgacgaggcg cacgcgcgca gcctggggct gaacacgggc tttctgcatt tcctgctcct
     1141 ggcgctgctc tccgccacgg cggtggcggc gctgcaaacc gtgggcgcct gcctggtggt
     1201 ggccatgctg gtgacgccgg gcgccaccgc ctacctgctg acggaccgtt tcggcaaaat
     1261 gctcggcatc gccacggccg cgggcgcggg ctgcggcgtg gccggcgcgt atgcgagctg
     1321 gtttttcaac ggctccacgg gcggctgcat cgtggtgctg cagacgcttg tcttcctggc
     1381 cgtgctggtg ctggcgccga agcacggcct gctcgcctcc cgccgccagc gccggctggc
     1441 cgcctccctg tcatgagcct gctcgaacct ttccgctacc ctttcatgac cgaggcgctg
     1501 ctggccggcg cgctggtcgg cgcggtgtgc gcgcttttct cctgctacct ggtgctcaaa
     1561 ggctggtcgc tgatggggga tgccatctcc cacgcggtgc tgccgggcat cgtgctggcg
     1621 cacatggcgg ggctgccgct ggcgctgggc gccttcgcca ccgggctgct gtgcgccgcg
     1681 gccaccggct ggatcaaggc ctcgacgcgc atcaaggagg acacggtgat gggcatcgtc
     1741 ttcaccggcc tcttcgcgct ggggctggtg ctgttcacca aagtggagac cgacgcgcac
     1801 ctgaaccaca tcctcttcgg caacatcctc ggcatcgagc gcgcggacat gatccagacc
     1861 gtcaccgcct cgacgctcac gctcgtcgtg gtgctgctga tgcgcaggga cctgatgatc
     1921 ttctgcttcg accccgcgca ggcgcgcgcc atcgggctga acacaggcct gctctactat
     1981 gtgctgctct ccctgctcgc cggcacggtg gtcgcctccc tccaggcggt gggcatcatc
     2041 ctcgtggtgg ccatgctcgt cacgcccggc tgcaccgcgc ggctgctgac ggaccgcttc
     2101 gaccgcatgc tgctgctggc cgcgggctcc tccgtgctgg ccacctgcgc cggagtgtat
     2161 gccagctttt tcatcaatgg ctccaccggc gcctgcatcg tgctggccca ggccgcgctg
     2221 ttcatgctgg ccttcgtttt cggcccgaag cacggcctgc tggcggcgcg gcgtgcggcg
     2281 tgagacgggc tcaccccagc aggtgctgga agacgcggct gatgagccag gcgtaaaaaa
     2341 tccacaggcc gatgatcatc gccgccagcg cggcaagcag caggcccagc gatagaaaca
     2401 accgcagggt gctctttttc accgccgcct ggttgcccgc ctgccaggcc tggagcaggc
     2461 gcagattgcg catgttggat ttgaaaaagg cgctcgccac agggccgccc aggggcagga
     2521 agtcgaagag ggtgttcagc aaaagattgc cgcccatgcg gatgaagacc ggcagcgtca
     2581 cgccgctgcg gatggcctcc agcaggatga tcacgccgcc gcccgatgcc acgccgctgc
     2641 ccaggccggg gatgagcgcc aggatggggt ccaggccgat cttgaaatcc gtgccgggga
     2701 tgcgcagcca gttgtccagc cacttcgcca ggtagcgcgc cagctccgac tcgcccgccg
     2761 ctctggccgc cattcccttt gccgaaggcg gcagcacctg gtctatgatg atgcggtcgg
     2821 ggatttctga aggcatggcg gagtgtaacg cacaagccgc gccaggccag cgtggatgcg
     2881 ggcggcttta gggatcaagc cgccgcctgg ttctgtccgc gcagtttttt cagatgcgcg
     2941 tagcccacca gcttcctgga ctgctcgtcc cacaagcgca gggaggcgca tttcaggctg
     3001 cccaggagcg tgaccggctt caccgcctgg aacaacgggt gggagtggtg gcagcgctcc
     3061 agattgtagt tcgggatgcg cgagctgaga tgatggatgt gatggtagcc gatgttgccg
     3121 gtgaaccact ggagaacctt cggcagcttg taaaacgagc tgccctccag agcggcggtc
     3181 gtgtagtccc agttctcgcg gttctcccaa taggtgtcct cgtattggtg ctgcacataa
     3241 aacatccaga tgcccgccgc gccggccacc agggtcacgg gcagttgcat catcagataa
     3301 gcctgccagc caaagagcca gcaccctgcc gccaccatcg ccgtcacgcc caggttcatc
     3361 cacatcaccg acgcgcggtc cttgcccttg gccccggagg agggaaagcg ctggtaaatc
     3421 cagaacagcc ccagcggcgc gatgacaaac agaacgaagg ggttccgcgc caggcggtag
     3481 gcgatttttt tccagcgcgg agcctccaga tactcccgca ccgtcagcgt ccagatgtca
     3541 ccctcgccgc ggcggtccag atcgcccgag gtggcatggt ggacgttgtg ctgccagcgc
     3601 cagtggccgt agggggtgaa ggtcagcatc ccggtgaaaa agcccagaat gtcgttggcg
     3661 ggcttcgatt tgaaaaagga gccgtggcag cagtcatgga aaatgatgaa cacacgcacc
     3721 agcagcagcc ccgccagcgc ggcgatgccg agcgtcagcc aataggagac ggaaaggctc
     3781 agccacatga gataccagag cgcgaagtag ggcacgagcg tgttcacgat ctgccacacg
     3841 gcgcgcggca tcgagggcgc cgcgtagctg gcgatgatgg ctttccattc ggaaatgccg
     3901 gcctggggtt tcacggcggg gggcggttgg gcgctggaca tcagggttgg gatgggagga
     3961 tcgagactga ctcgcaaacc aacgacctta cacgcctttc cttccacgca caatttggca
     4021 aacaagtttc tttttctgcg ccgctcccct ggggcgcggg tccgaaaagc cgcctgaatc
     4081 aggccgccgc agccatcctc agtcccccaa accgctcgcc gtgccggtga cctccccgtc
     4141 gtcgagttcg aatcagatgc gcatggggcg gcagcagact tcgcagtcgt aatccacatc
     4201 gcaaggcgtc tcatccggcc agggagcggg cacttcgaac tcctggaagc aggacgggca
     4261 ggtgacggga gtggtggtca tcggggattc acagattggg aaacaaacaa agggcatctg
     4321 ccggcaaggg cgcgcggatg cgtgatacct ggattttttt ctttattgtg cgccccggtc
     4381 tttgatacgc ttggctaaat cgagctattc atccatgaag cgaagaagtt tcctcacaac
     4441 ggcggccgcc actctggcga gtccgctatt ggctgaaaat actccgcccg cccgtcttgg
     4501 agtgcctttg taccaggagc ccctcccgtt ccgtttcgaa gacttgcgcc catgcttcag
     4561 tgcggacgct ctgcggcggc attacgaact gcatcatgcg aatcacttga gcaaattgaa
     4621 ggagactctt gacgcccaga gcatctctgt cggcaatgtc gccgcattga tgtcgaacat
     4681 cgaggggatc attcgtccgt cgaattcccg ctcggtgctg ccgcttgggc ggctcatcag
     4741 tctgggcatg tcgggatcat ccgaccacgt cctgccaccg aaaactgtgc gcgtgatccg
     4801 ccaatccggc ggaggtcatg tcaatcacac ggccttctgg cgttttctcg gccctcatgg
     4861 gtcgggtccc tcgggaccgc agggagattc ggcccgcgcc atcagggaac attttggcag
     4921 tgtgaggaac ttccggcgtt tcttcaagga agtggccatg tcgcatgtcg gttcaggctg
     4981 ggcctggctt gtgtttcgcc aggacaaggc tctcgtcgtt acaaccacgt tcaacgagga
     5041 caatccgctc atgaaggaca tcatcccctg gcatcaggcg ggacgcccca ttctggcgct
     5101 ggacctctgg gagcacagtt attacgatgc gcacggcgat gaccgggaga gctatgttga
     5161 ctcctggtgg aaggtcgtga actggacttt tgtcgaacgc gcctaccaga tcgtcaccaa
     5221 aataagttga tctcgtcttg cgagatcaga cggtgccgca attgcatggc tgggagtggc
     5281 gaggcatgcc aaccagccgg gaggacaggc atttcgaact cctggaagca ggacgggcag
     5341 gtgacgggag tggtggtcat gatgatgaga aaaaaggaag ctcgaaactc gaaacatgag
     5401 attcggtctt cgaatttcga atttcgattt tccccttcca cgctcatgtt gcctcgcatg
     5461 aaactcctgc gcttcctcct ccatctggcc ttcggcggcg tcttcgtcta tgcgggcgcg
     5521 ctcaaggccc gggatcccgc cgcctttgtc atggatgtgc gcagctttga actgctgccc
     5581 gacccttggg ccgcgtggct cgccatgtgt ctgccctggc tggaaatctt cgccgggctc
     5641 gccgtcatca ccggcgtgct gcgcgcgggc ggcctgctcg tgctcaattc cagcctgctt
     5701 gccttcctcg cggccatcct cagcgcctgg gcgcgcgggt tggacatcca gtgcggctgc
     5761 ttcggcggcg gcgcgggcac gagcagccac gtcgaactca tcgcgcggga tgtggcgctg
     5821 ctggcgctgg gaatgtggct ggcctgctcg cgcggaaaag gatgaggcaa ggcggactgg
     5881 cggccgggcg ttttcttcgc ctgccatgaa actcgtcccc cttgtcctgt catgcctggg
     5941 tctgtccgca gccagcgccg ctgagaagct caatgtcgtt ttcattctcg ccgacgacct
     6001 cggctggggg gaggtgggtt gctacgggca gaaaaaaatc cccaccccga acctcgaccg
     6061 catggccgcc gagggcatgc gcttcaccca gcattacagc ggcgctcccg tctgcgcccc
     6121 ctcccgctgc gtgctcatga ccggcaagca tctcggccgg gccgaggtgc gcggcaatct
     6181 ctccatgccc aaagtggacc cgaagtatga cgaagggcag tggcccatct cggagaacgc
     6241 cttcacgctg gcgatggcct ttcaaaaagc cggttatgcc accggcgcca tgggcaagtg
     6301 gggtctcggc ccggtcggca gcaccggcga gccgaacaac aaaggctttg atctcttctt
     6361 cggttacaac tgccagggcg tggcccacag cttttacccg ccccacatct ggcggaacac
     6421 cgagaaaatc atcatcaaca agaatcccat ccccggccac gccaaacagc ccgagggtga
     6481 aatcaagctg gaggactgga tcggtgaaac ctacgcgccg aagctcatga tcgccgaggc
     6541 tgagaaattc atcgagagca gctgcgcgcg ccccttcttc ctctacctgc ctttcatcga
     6601 gccgcacgtc tccatgcatc cgccgaagga gtccgtggac aaatttccgg ctgaatggga
     6661 cgacgaggcc taccggggtc aatgcggcta cgtgccccac ccgcgcccgc gcgccggtta
     6721 cgccgccatg atcagcgatc tcgacggcta tgtgggccgg gtgatgaaaa agctggagga
     6781 aaccggtgtc gccgaccgca cgctggtgat cttcagcagc gacaacggca ccacccatcc
     6841 ccgcctgccc gccacgcatt ttcatgtcgg cggcgtggac ggtgccttct tcaacagcac
     6901 cgccggcctg cgcggctgga agggcagcgt ctatgagggc ggcctgcgcg tgcccacact
     6961 cgcgaagctg cctggcgtca tccccgccgg ccgggtcaat gaaagccccg gttacttcgc
     7021 cgactggttc cccaccctct gcgaggccgc cggtctggaa aaaccggacg gactcgatgg
     7081 cgaaagcctc tggcccgtgc tcaccggcca ggtggcgaaa caagcgcgct cccgaccgat
     7141 gatctgggtc ttccccgaat accagggcca ggtggccatc cgcctcggcg gcatgaaact
     7201 cgtgcgccag aacctcaaaa ccaaagcgcc aggtccctgg gaagtctatg acctggacaa
     7261 agaccccggc gaaagcaacg acctctccgc ttcgcactcc gccctcatcc gtgaggcgga
     7321 agaagttctg cgccgcgagg tcaacgacaa cgagcgcttt ccgctcgaca tccctggtgt
     7381 gacgacagcg gcggcgaagt aaccgccagg tacgcccccc tgggaatcac cccctctgcg
     7441 gcctgtccag tctcagggtt ttcagtccgc agccagtcag tccggcgggc catgtttctt
     7501 caaatgcggc ggcgcagctc acgcctttct cagcggccct cgttttccct ggccggctcc
     7561 gcaggctggg acttgaaaaa cttcacgatc acttccggcg cctcggctgg gtatttgtgc
     7621 gtgccggggt gaatggcagt gagcaccggg catccgactt tggagggatg aatgctgatg
     7681 cggtcgtcca gctcccaggg cccggattcg ccgcactggt tgagcgcgag aagcgaggcg
     7741 atcatggctt gctgccagga gtatttgacc agttcatcat tctcacccgc gatgtggagg
     7801 accggcttgg gttttagcag gccgcggacg cgacccgccg cggaggcgga aggagccatg
     7861 gcggcaaaga cctccccgcg cgccgcccaa agcatgtagg tgaagccgcc gccattggag
     7921 tggccggtgg aataaatgcg gcgcgtgtcc accttcagtt ccgtcttcat cgtggcgagc
     7981 atggcgtcga aaaacttcag gtcgcggtcg cccagttcgc cgatggaatg ctgccagccg
     8041 ggttttttcc cctcggggtc ggtgagctgc cccggcgtgt tcagcccctg tggatacacc
     8101 acggcagcct ccggccaaag ggaatggatg cggtagctgc gggcggcatt ggccatgctg
     8161 ccgccatgcc cgtgaaagcc gaacaccagc ggacagggct tttccctggc gtttgccgga
     8221 acatgcacca gcgcttcgcg gatgactcca tccacggtcc attgccgccg ctgcaaagcg
     8281 ggaacctgcg cggggaggga gaggctgctg agagcgactg ccagcgcggg cagccagagg
     8341 atgaggaaac ggagtctgga catcgccggg atgcaacctg cgggcgaatg acaggttgcg
     8401 cgcgggttgt ggcggctttt ccccacaggt cgccgggttc gatgaaaagc tcctcccagc
     8461 ggcgcatccg catcatcgct tcaatcggcg gctcagatga ccacccggaa ctcgtcccgg
     8521 tccgtcacgc tttccgcgaa ggcggtgagc agttggatga cctgctcaat gtcacgcaga
     8581 tccgccatct cgaccacgct gtgcatgtag cgcagcggca gcgagacgag cgcgctgggg
     8641 atgccgtgct gctggtggaa gatgacatcc gtgtccgtgc cggtgaaacg ggaggaggac
     8701 tcatgctgga tggggatttt gcgcttttcc gccacttcga tgagacggcg cacgacctcg
     8761 atgtggttcg cgccgccgtg tgtcaggctg gggccgccgc ccagtttcac ctcgccgtgc
     8821 tttttcacat ccaccgaggg tgtgtccgtg gcgtgagtca catccaggac aatggcgaca
     8881 tccggcatca gacgataggc ggccatcttc gccccgtggc cgccgatctc ctcctgcacg
     8941 gcgttcaggg cgaccactgt cgccgcgggg cgcgtcttct tttgggcgag tctggacacg
     9001 acctcggaga tgatgaagcc gcccacgcga ttgtcgagcg cgcgccccac cagccggtgc
     9061 gcgccaagct cttcgacggt gtcggaatac acggcgggat ggccgacgcg aatgcccgca
     9121 ctggcgacct ccttgtcact ggcggcgccg atgtccatcc agagttcgtg gactttcggc
     9181 actttttcat cgtcgcgatt ttcacgaatg tggatggcga tgttgccgat gatgccgcgc
     9241 accacgcctt tgtctcccag gatgtccaca cggcggccgc gcgcggtggc cacatcactg
     9301 ccgccgacac ggtccacatg gatgaagccc tccttggtga tgtgcttcac catgaagccg
     9361 atctcgtcgg catgcgcctc gaacatcacc cggcgcggtt ttttcccgcc gccatccagg
     9421 gtggcccatg cgctgccgta ggcgtcgctt tccacccggt cggcgtgccc gcgcaaatgc
     9481 tccgcccatt tgcgctggcc gcgaaattca aagccggtcg ggctgggtgt ggagagaaga
     9541 tcgagaagaa acgtcttgga ggctggggac atcccgcaag gaaaagcgga aatcgaaatt
     9601 cgatcaagcc tcactttgca gcggacgcga cgggcacttt gagcacgcct tgcagcggtt
     9661 tcgggaagac cccgatgacg aggatggctc cggcgagcag gacgagaacc gccctggtcg
     9721 gcgcggcgat tttcaatgcc gggccgcttt cacccgtgaa catgacgagc acgggcctca
     9781 ggtagtaata aaagccggcc gccgcgccga tcaccgcgca cgagagggcg aaccagcggg
     9841 cgttttccac aaggccgagg aagacgaaca gcttgcccat gaacccggct gtcagcggga
     9901 tgccagccag cgaggcaaaa cccagcgtgg ccgcgaaggc gaggaagggg ttctttttcg
     9961 ccagccctgc gaagtcctgg gcgtcatcgc cgaggcccgc ctggcgcacg acggtaagga
    10021 tgaggaagcc gagcaccgtc atcggcaggt aagtgccaag gtagaaggct acgacgctgc
    10081 tgccgccgag gtcaaaacgc gctgtctgag agcagccgag cgccagcagc aggtagccgg
    10141 catggctgat gctggagtag gccagcaggc gcttcaaatt tgtctggaaa atggcgggca
    10201 gactgccgag caggacggtg agagcgccgc cgatgagcag gagcgcctgc acttcggagg
    10261 cgatgaccga gcctttggcc aggaaggcat ccaccacgcg ggtgagcaca atgaagccgg
    10321 cggctttcga tcccaccgaa aggaacgcgg tcacaggatt cggcgcgcct tgatagacat
    10381 ccggcaccca aatgtggaag ggcgcggcgg cgactttgaa tccgagtccg gccagcaaaa
    10441 aggccgcgcc aagcagggcg gcgtttttgg ccgcggcggg cgcggcgagc gcttcggcga
    10501 tgccttcaaa gctcatggaa tccgtgacac cataggtcca ggcgatgcca taaacgagca
    10561 caccggtgct caacgcgccg aggatgagat acttcactcc cgcctccagg gcgaagctgc
    10621 ttttgcgcgc gaaggcgacc agcacataga aggaaacggt gacgagttcc agacagacaa
    10681 aaacggtgat gaggtccttg gcggaggcca tccacatcat gcccgcgcag acgatgagcg
    10741 gcaggctgaa catttcccca tgcctgccgc caggggtgta gcgctccaga taagcgcggc
    10801 tctcgacggt gagccacagc acggcgagcg tgatgacgag ggcgaagccc ttgtaaaaca
    10861 ccgccaatgt gtcgagctgg tggaagggct gcacgaatgc cggcagagcg gcggcggatt
    10921 tgtcggcaaa aaagaacag
//
LOCUS       DAOMYJ010000181        10937 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Prosthecobacter sp. isolate AMR_MDS_5629
            NODE_2001_length_10937_cov_18.794339, whole genome shotgun
            sequence.
ACCESSION   DAOMYJ010000181 DAOMYJ010000000
VERSION     DAOMYJ010000181.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867850
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prosthecobacter sp. (wastewater metagenome)
  ORGANISM  Prosthecobacter sp.
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Verrucomicrobiaceae; Prosthecobacter.
REFERENCE   1  (bases 1 to 10937)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10937)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.30
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:34:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,742
            CDSs (total)                      :: 4,684
            Genes (coding)                    :: 4,676
            CDSs (with protein)               :: 4,676
            Genes (RNA)                       :: 58
            tRNAs                             :: 55
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10937
                     /organism="Prosthecobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2001_length_10937_cov_18.794339"
                     /isolate="AMR_MDS_5629"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1965333"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(60..773)
                     /locus_tag="PLP58_16355"
     CDS             complement(60..773)
                     /locus_tag="PLP58_16355"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: protein motif:HMM:NF014409.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acylneuraminate cytidylyltransferase family
                     protein"
                     /protein_id="HRJ10153.1"
                     /translation="MKNIICTVCARGGSKGVPGKNTRLMHGIPLIQHTLRQAAASGIF
                     TTIVASSDSEAILDCAAEVSGVRTLLRPAGLASDTAGKVPAIRHAMLAMEERLGAPCD
                     ITVDLDVTSPLRSPDDIRAAVALLQDGVPNVLTAMHSRRSPYFNLIERHPDGQIRTAK
                     ALPGGVLRRQDAPPCYDMNASIYVWRRDVLAAEDRLFLPGTALHLMPEERSWDIDSEM
                     DWHVVELLMSLQPPAPRPA"
     gene            complement(770..1717)
                     /locus_tag="PLP58_16360"
     CDS             complement(770..1717)
                     /locus_tag="PLP58_16360"
                     /inference="COORDINATES: protein motif:HMM:NF013567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HRJ10154.1"
                     /translation="MSQSRPRTLIIGLGSIGQRHADLLADLGAEVAACTRRRDAGLPS
                     YTGLEQALRESAPERVVIANPTAEHVGTLLRLHELGFSGDVLVEKPLFSQIPETPLPA
                     SFRQSVAVGYNLRFHPAVRALREALQAEEPVSAHLYVGQYLPTWRPGRDYRKSYSASV
                     AQGGGVLRDLSHELDLGLWLFGPWQRLIAHGGHLSELEIETEDTVGLLWQARHCPKVA
                     VEINYLDRAPRRNILVHTRRHTWEADMLGCRLLRDGEPVQSWTTIRNATYQAQDRAWL
                     DGQRDTLCSFDEGLAVLHMIESIEASLRRCAWVDAPSPP"
     gene            complement(1714..2514)
                     /locus_tag="PLP58_16365"
     CDS             complement(1714..2514)
                     /locus_tag="PLP58_16365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ10155.1"
                     /translation="MFEFAAGLDVVLAAADFIFFELGMAAPLFSDHPPSPHTGAGLTG
                     MVIEPGLDKLRNLVIRQLSNVFEFDGGEDSAALDSGIHSALGRVERCFSGIVLKRYRR
                     EDGAVVFNPFHSGQYCIFLYYLGRELSLQGAAGLADRVYYLNRALNGVDLFHQVELPD
                     VFCVEHPLGSVIGRARFGNGFFFAQGVTVGGNKGAYPVIGAHVSMLSNSKVVGASVIG
                     DHVILSANSYVKDTVIPPHSIVFGQDRNLVIKPNPGGLTAEIFHSSVA"
     gene            2982..3983
                     /locus_tag="PLP58_16370"
     CDS             2982..3983
                     /locus_tag="PLP58_16370"
                     /inference="COORDINATES: protein motif:HMM:NF014893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HRJ10156.1"
                     /translation="MASANSDRPRKIAQIGTAHAHASGKMSAMRALPELWEVLGVSET
                     DPELRRLAEKSSAYAELPFLSEAEILNQSDLTTVVVETRIEDSCTTALNCLRAGKNVH
                     LDKPGALDHADFKAMRLEAERLGLVVQMGYMLRYNPAFELLFQSVKEGWLGEITEIDA
                     AMGKLADPGTRKTLAALEGGGMFELACHVIDAAVTILGKPREVRAFSTPVGDDGTKDN
                     QTAVLVYPHATAVIRCNHADPFGGPRRRFNVTGTEGTLEIVPLESGRINLSLSKARGN
                     YRKGHQSIEIEIPKGRYDREFVDLAEVVAGKKPLAWDAVHDIAVHETVLRAAGVWGE"
     gene            complement(3993..5174)
                     /locus_tag="PLP58_16375"
     CDS             complement(3993..5174)
                     /locus_tag="PLP58_16375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009960222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HRJ10157.1"
                     /translation="MRRRAFLGTTLAAASMTGRGLADEARLRVAVIGHTGRGNFGHGL
                     DTMWKDVPGVTVVAVADADVKGLAAAQAKLETGQGFTDYRRMLAETKPDIVAIGPRHI
                     DQHHAMSLASIEAGARGIYMEKPFCRTLREADEIVAACARSGAKLALAHRNRYHPVLP
                     VLADLLRQGKIGRVLEYRARGKEDARGGPLDLWVLGCHLFNLIHFFAGEARTCSATVL
                     QEGRPVTAADLSEGAEGVGPLAGNEVHARFEMADGLPAFFDSVQNAGVRDTGFGVQII
                     GTEGVLDLRVDAEPLAHWRAGSPFRPIASSSAGWIPVTSGGPGVPEPVKDIRKQVGGH
                     LAPALDLIAAIQEGREPLCSAADGRHALEMTAGVFESHRQKGARISLPLKEREHPWMR
                     L"
     gene            complement(5171..5797)
                     /locus_tag="PLP58_16380"
     CDS             complement(5171..5797)
                     /locus_tag="PLP58_16380"
                     /inference="COORDINATES: protein motif:HMM:NF028007.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051219 - phosphoprotein binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HRJ10158.1"
                     /translation="MPTLVFHLDDGSVLTHELEDGVTTLGRHPDSVVVLEFPSVSAHH
                     AIIEMGEGGCFVSDQKSSNGTRVNGVVIEESRLKEGDRVAFGDVQAVFYQGAPPEMVE
                     APEPEVIYVPAPEVPAPESPPPMPQRNYRQPKPRRNPKVKVTSYPDTSDSGCATAVIV
                     IALFVTAFIIGLAMRHSKETGGNFITDMLDKISGAVPKITIEKKVDEP"
     gene            5939..6391
                     /locus_tag="PLP58_16385"
     CDS             5939..6391
                     /locus_tag="PLP58_16385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ10159.1"
                     /translation="MFFLPRPSLLIPALMLLAFFAPLSAGEADDAESAALALVNDQEK
                     QGFTFRAETWVAPLSKDFGKAVRMQMFKGNEYCVAVAVPRKSGIHVKGEVLDLDGKPV
                     GEIQPVLEGWGFLLFFKPKKTGVYVATVRMEESGKLKDTHCALITGYR"
     gene            complement(6407..6511)
                     /locus_tag="PLP58_16390"
                     /pseudo
     CDS             complement(6407..6511)
                     /locus_tag="PLP58_16390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010884088.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
     gene            complement(6488..6592)
                     /locus_tag="PLP58_16395"
                     /pseudo
     CDS             complement(6488..6592)
                     /locus_tag="PLP58_16395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612204.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
     gene            complement(6818..6949)
                     /locus_tag="PLP58_16400"
     CDS             complement(6818..6949)
                     /locus_tag="PLP58_16400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ10160.1"
                     /translation="MPKHICSTPPVMDTLTMAADPGGRVLTASPCAPVTRARRPSNP"
     gene            7252..7794
                     /locus_tag="PLP58_16405"
     CDS             7252..7794
                     /locus_tag="PLP58_16405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ10161.1"
                     /translation="MTAVPLHFLVGITRLISIHFALKSLDSIGAAVVAYGMQISVSPE
                     MARQFPSVWALYLPSLAIYLGLVVLTWFAAPGICRLAMRSGLPVETREDTCEKVAWGE
                     IMIFLTGILLMCWGISRAAAGIMPILQAASRELHHELVLADQLEFFVAFALTGAGGLL
                     AARFPSVYRWCERRKREAGS"
     gene            complement(7817..8527)
                     /locus_tag="PLP58_16410"
     CDS             complement(7817..8527)
                     /locus_tag="PLP58_16410"
                     /inference="COORDINATES: protein motif:HMM:TIGR02206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02206 family membrane protein"
                     /protein_id="HRJ10162.1"
                     /translation="MPPYDFQAFSRSHIAVLAACLAALVLLAFLRRVNESQAVRAERV
                     LGFLLLLIWPLTVLAYWHTGILSLGNALPLHYCDIAGIAAGLALLTRRQTACEVVYYL
                     GLAGTLQGLLTPNLKADFPDPRFLVFFLLHGGVVVTALHVVTSMGRAPRPGALRRMLA
                     FTLGYAALTGLINTALGTNYAFLCRKPEQASLMDHLGPWPWYIGSLVLLAFVLYSLLH
                     LPFHLARRKAAPDGDAAA"
     gene            8624..9781
                     /locus_tag="PLP58_16415"
     CDS             8624..9781
                     /locus_tag="PLP58_16415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018969104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HRJ10163.1"
                     /translation="MNPPANVVELCQALVRIPSVNPDGDPGTARTGEAECARFIAAFL
                     ERCGAEAFLDEVEPDRPNVIGRFPSLPRPGGGAKPRIVFGPHTDTVSVGGMSIEPFGG
                     ELRDGRVWGRGASDTKGPMAAMLWALHEMRARIPALPVEVHFAGFMSEESAQLGSQHF
                     ARHHGSYALAVVGEPTELRTVFRHKGCLWADVVTHGVAAHGATPERGENAIVKMARLV
                     HALDTDFRALLVEAGGVDEWLGASTINLGMIQGGARSNIVADRCALRVDLRTTPGLNR
                     AGGALRLLADFIRRLDPGAEVLPLPETFPLDTDPENPLVQTLVKCGAPLAGAPWFCDA
                     AFLAAAGIPAIAIGPGSIAQAHTKDEFISAADLEAGAAFFVRFLQSLEAGC"
     gene            complement(9955..10608)
                     /locus_tag="PLP58_16420"
     CDS             complement(9955..10608)
                     /locus_tag="PLP58_16420"
                     /inference="COORDINATES: protein motif:HMM:NF017496.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Uma2 family endonuclease"
                     /protein_id="HRJ10164.1"
                     /translation="MPATVPAPASRSQAHSSPLENGELVALPEFLRRFEKMQGVKKAE
                     LIEGVVHMPSAVRLNHSQPDGIIHGWLFAYAAEHGLEMLPNTTLLLDTENCFQPDAIL
                     CGTPRRGGRVWVNEAGYLCGSPEMVVEVAASTGSIDLRDKLRVYRRLEVAEYLVWRTE
                     DKEVDWFQLEAGAYIRMKPDRHGRLRSRIFPGLVLDVEAALRGDKAAVLAALKKRGR"
BASE COUNT         1972 a         3273 c         3636 g         2056 t
ORIGIN      
        1 ggcgccgccg gtgatgaggg cggtgcggcc ctggaggctg gcgagctggg cgagggtgct
       61 catgcggggc gtggggcggg gggctggagg ctcatgagga gctcgacgac gtgccagtcc
      121 atctcgctgt caatgtccca ggagcgctcc tcgggcatga ggtggagggc ggtgccggga
      181 aggaagaggc ggtcctcggc ggcgaggacg tcgcgccgcc agacgtagat ggaggcgttc
      241 atgtcgtagc acgggggggc gtcctggcgg cggaggacgc ctccggggag ggctttggcg
      301 gtgcggatct gaccgtcggg gtggcgctcg atgaggttga agtagggtga gcggcgggag
      361 tgcatggcgg tgaggacatt gggcaccccg tcttggagga gggcgacggc ggcgcggatg
      421 tcgtcggggg agcgcagggg ggaggtgacg tcgaggtcca cagtgatgtc gcagggggcg
      481 ccgaggcgct cctccatagc gagcatggcg tggcgtatgg cgggcacttt gccggcggtg
      541 tcgctggcga ggccggcggg acggaggagg gtgcggacgc ctgagacctc ggcggcgcag
      601 tcgaggatgg cctcggagtc gctgctggcg acgatggtgg tgaagatgcc gctggcggcg
      661 gcctggcgca gggtgtgctg gatgaggggg atgccgtgca tgaggcgggt gtttttgccg
      721 ggcacgcctt tggagccgcc gcgcgcgcag accgtgcaga tgatgttttt catggggggg
      781 aaggggcgtc cacccaggcg cagcggcgca gggaggcctc aatggactcg atcatgtgga
      841 gcacggcaag gccctcgtca aaggagcata aggtgtcgcg ctgaccgtcc agccaggcgc
      901 ggtcctgggc ctggtaggtg gcgttgcgga tggtggtcca gctttgcacg ggctcgccat
      961 cccgcagcag gcggcagccg agcatgtcgg cctcccaggt gtggcggcgg gtgtggacga
     1021 ggatgttgcg gcgcggggcg cggtcgaggt agttgatctc gacggccact ttcgggcagt
     1081 gccgcgcctg ccagagcaga cccacggtgt cctcggtctc gatctccagc tcgctgaggt
     1141 ggccgccgtg ggcgatgagg cgctgccacg gtccaaagag ccacaggccg aggtccagct
     1201 cgtggctcag gtcccgcagc acgccgccgc cctgggccac ggaggcggag tagctcttcc
     1261 ggtagtcccg ccccggtcgc caggtgggca ggtattgccc cacatacaga tgcgcgctga
     1321 ccggttcctc cgcctgcaag gcctcgcgca gtgcccgcac cgccgggtga aagcgcaggt
     1381 tgtaccccac tgccacgctt tgcctgaagg aggcgggcag gggcgtctct ggtatttggc
     1441 tgaagagcgg cttctccacc agcacatcgc cggagaaacc cagctcgtgc agccgcagca
     1501 gggtgccgac gtgctccgcg gtggggttgg cgatgacgac gcgctcgggc gcggactctc
     1561 gcagcgcctg ctcaagcccc gtgtagctgg gcagcccggc atcccgccgc cgcgtgcagg
     1621 ccgccacctc cgcgcccagg tccgccagca gatccgcatg gcgctggccg atggagccga
     1681 gtccgatgat gagagtgcgg gggcggctct ggctcatgcg acgctgctgt ggaagatctc
     1741 cgcggtgagg ccgccgggat tgggtttgat gacgaggttc ctgtcctggc cgaagacgat
     1801 ggaatgcggc gggatgacgg tgtctttgac gtagctgttg gcggagagga tgacatggtc
     1861 cccgatgaca gaggcaccca cgactttcga gttcgagagc atgctgacat gcgccccgat
     1921 cactggataa gcgcccttgt tcccccccac cgtcaccccc tgggcaaaga agaaaccatt
     1981 gccgaaacgc gcgcgcccga tcaccgaccc cagcgggtgc tcgacgcaga acacgtcggg
     2041 cagttccacc tgatggaaga ggtccactcc attgagcgcg cggttcaggt aatacacccg
     2101 atccgccaga ccggccgcac cttgcagcga cagttctctt cccaggtaat acaggaagat
     2161 gcagtattga ccagagtgga agggattgaa gaccaccgcg ccatcctccc ggcggtaacg
     2221 cttcagcacg atgccggaga agcagcgctc cacacggcca agtgccgaat ggataccgga
     2281 atcaagcgct gcagagtctt cgccaccatc gaattcaaag acattgctca actgacgaat
     2341 caccaagttc cgcagtttgt ccaaacccgg ttcaatgacc atgcctgtca aaccagcccc
     2401 ggtgtgaggg gaggggggat gatctgaaaa tagaggcgca gccatgccta gctcgaaaaa
     2461 aataaaatca gccgccgcca acaccacgtc aagcccggcg gcgaattcga acatgtctcc
     2521 gttcgttatg ccgatttcca ttcagcggtg tctgctatcg gaaatcataa tgcgcttcat
     2581 gacgtgggcg ggcgggtgac gcaccttgaa atggccgcat gcagattgaa ccatgagcgt
     2641 gcaagatttc tttcttatca tccagaacgc agggtaagtg ctccaaacca cttcattcct
     2701 cccaattcgg ctagatcgtt ccaattttat aactacacac aatatatgta atgtaaaata
     2761 gttgggatgt ggggcagttt gaatacggga attgtgaaat agctcggctg cattttggtt
     2821 ggatgtttat gtcagtagaa tggcagaggc tgattctttt gaactcatct ttggacaagt
     2881 ttgcattcag aaaccgtttg gattaaatgc tccgtcgtcg tgtgctttca tcctccctgg
     2941 ccatgctgtc tgtcaatctg acaatgagtc ggtcgcagtc tatggcatct gccaattcag
     3001 accggcctcg gaaaatcgca caaattggga ctgcgcatgc acacgcctcc ggcaaaatgt
     3061 cagccatgcg agcgcttccg gaactttggg aagtgctagg cgtgagcgaa actgacccgg
     3121 agctgcgtcg tttggcggaa aaatcgtcag catatgcaga acttccattt ctgagtgaag
     3181 ccgaaatttt gaatcagagt gatttgacga cagtcgtcgt ggagacccga atcgaggatt
     3241 cttgcacaac ggccctgaac tgcttgcgag ccggaaaaaa tgttcacctg gacaagcctg
     3301 gagcactgga tcatgccgat tttaaggcca tgcgtttaga ggctgagcga ttgggactcg
     3361 tagttcagat gggctatatg ctgcgttata atcctgcttt tgagcttctt tttcagtcag
     3421 ttaaggaagg gtggttgggt gagatcacgg aaattgatgc cgcgatgggc aagctcgccg
     3481 accctggcac ccgcaaaact ctggcagcgc ttgaaggagg cggcatgttc gaacttgcct
     3541 gccatgtgat tgacgctgcg gtgacgattc tgggcaagcc tcgtgaggtt cgcgcttttt
     3601 ccactcctgt tggagacgat ggaaccaagg acaatcagac cgccgtgctc gtctatcctc
     3661 acgccacagc ggtcatacgt tgcaatcatg ccgacccttt tggtggtccc aggcgccgct
     3721 tcaatgtcac gggcacggaa ggcacccttg agattgttcc cctggagtca ggacggatca
     3781 atctgtcatt aagcaaggca cgcggaaatt atcgaaaagg ccatcaatcg attgaaattg
     3841 aaatacccaa aggcagatat gatcgcgagt ttgtagattt ggcggaggtt gtcgccggga
     3901 aaaagcccct ggcctgggat gcggtccacg acatcgcggt gcatgagaca gtgctgcgcg
     3961 cagccggtgt ctggggcgag tagaacggcg gttcagagac gcatccaggg atgttcgcgc
     4021 tctttgaggg gcagggaaat gcgcgcccct ttctgacggt gcgattcgaa aacgccggcg
     4081 gtcatctcca gcgcatgtct gccatcggcg gcggagcaga gcggttccct gccctcttgt
     4141 atcgctgcaa tgaggtcgag agccggggct aggtggccgc ctacctgctt gcggatatct
     4201 ttgacaggct caggcactcc cggaccgccg ctggtaacag gaatccagcc tgcggaggaa
     4261 ctggcaatgg gacggaaggg gctgcccgcc cgccaatgcg cgagcggctc ggcatccacg
     4321 cgcagatcga gcacgccctc ggtgccgatg atctgcacac cgaagccggt gtcgcgcacg
     4381 ccggcatttt gcaccgaatc aaaaaacgcc ggcagaccgt cggccatttc gaaacgcgca
     4441 tggacttcgt tgccagcgag cggccccact ccctcggcgc cctcggagag atccgcagcg
     4501 gttacgggtc tgccttcctg cagcacagtg gcggagcatg ttctggcctc gcctgcaaag
     4561 aaatggatga gattgaacag atggcagcca agaacccaaa gatccaacgg tccgccgcgc
     4621 gcgtcctcct tgccccgcgc ccggtattcc agcacacgtc cgatttttcc ctgccgcaac
     4681 aaatctgcca ggaccggcaa tacggggtgg tagcgattgc ggtgagccag ggccaacttg
     4741 gcgccggagc gcgcacaagc ggcgacgatt tcatctgcct cacgcagtgt gcggcaaaag
     4801 ggcttctcca tgtagatgcc gcgagcgccg gcttcaatgg aggccaggct catggcgtga
     4861 tgctggtcaa tatgtctggg accgatggcc acgatgtccg gcttggtctc cgcgagcatc
     4921 cggcggtagt cggtgaagcc ctggccggtc tccaacttcg cctgcgcggc ggccagtcct
     4981 ttgacatccg catccgccac ggcgaccacc gtcactcccg gcacatcttt ccacattgtg
     5041 tccagtccgt gcccgaaatt accccttccg gtatggccga tcaccgccac gcgcaggcgg
     5101 gcttcatccg ctagtcccct gccagtcatg gaggctgccg cgagggtggt gccgagaaat
     5161 gcgcgccgcc tcatggctcg tccacctttt tctcgatggt gatctttggc acggcgccac
     5221 tgattttgtc gagcatgtca gtgatgaagt ttccaccagt ctccttggaa tgccgcatgg
     5281 ccagtccgat gatgaaggcg gtcacaaaca gcgcgatgac gatcaccgcg gtggcacacc
     5341 cgctgtcgga ggtgtcagga taggaggtga ccttgacctt ggggttcctg cgcggttttg
     5401 gctgccggta attccgctgc ggcatgggcg gcggagattc cggggccggc acctcagggg
     5461 cgggcacata tattacttcc ggctcgggcg cttccaccat ttcggggggc gcaccttgat
     5521 aaaaaaccgc ctgcacatcg ccaaaagcca ccctgtcgcc ttccttgagc ctggactcct
     5581 caatgaccac gccgtttacc cgagtgccgt tgctcgactt ttggtccgag acaaagcagc
     5641 ccccttcgcc catctcgatg atcgcatggt gggcggaaac cgacggaaac tccagaacca
     5701 ccacactgtc aggatgcctg ccgagcgtcg tcaccccgtc ttccaattcg tgggtcaaaa
     5761 cagagccgtc atccaggtga aaaacgagag tgggcatgcg tgcgtaatgt ttgcgggatt
     5821 gtgaaagcca acggaggaca aactgagggc atttcaatac aaagcacaca gaatcttggc
     5881 tttttgatga tgcaatgcct atgttcgacg tacagcttgt ttcctgccaa acgccgccat
     5941 gtttttcctc ccccgacctt cccttctgat cccggcactg atgcttctgg ccttcttcgc
     6001 gcctctgtcc gccggtgagg cggatgacgc cgagtccgcc gccctggctt tggtcaatga
     6061 ccaggaaaag cagggcttca ccttccgggc cgagacatgg gtggctcctc tatcgaagga
     6121 tttcggaaaa gccgtgcgca tgcagatgtt caagggcaac gagtactgcg tggccgtggc
     6181 cgtgccgcgc aaatcaggca tccatgtgaa gggcgaggtg ctcgatcttg atggcaagcc
     6241 tgtcggcgaa atccagcccg tgctcgaagg ctggggtttc ctgcttttct tcaaacccaa
     6301 aaagaccggc gtttatgtcg ccacggtgcg gatggaggaa agcggcaaac tcaaggacac
     6361 ccattgcgcc cttatcactg gttatcgtta gagcgtgtca tgcactttag aagaggtttt
     6421 tgagcatgag gaagacgctg gcgggccagt gcagaccggc gagcgagtcg ggcaggcgtt
     6481 catagtcgcg gctcagacgg cggaaacgcg cctgctggtg gatggcatgc caaggaggcg
     6541 gcagatcatg gggcagcatc cgccaggggc agcctgcgcg cacgatgtgg cgcggccgtt
     6601 gaagttcgcg cgaggaatgc tcgcgctgcg gagcgtcctc tctctcatca agcacagata
     6661 cggcccgcaa aaagcccatt cctcgtcact gacatcgctg ggataactga ctcttctccg
     6721 gctcgtcggc ctctttggtt cttggggcat ccgcagctca tgctcgcgcc ttgttcgagt
     6781 gtgcggcgct ttttcacctc gaagttcata acacgctcta aggattcgac gggcgccgcg
     6841 cccgcgtcac cggcgcgcag ggggacgcgg tgagcaccct gccgccgggg tctgccgcca
     6901 tcgtcagcgt gtccatcacc ggtggcgtgc tgcagatgtg cttcggcatc ccacgcggac
     6961 tgggcggtga tcaaggcccg cccggcgagg tgagcgaagc gcagcccaac gacgccatcg
     7021 ccaccacgcc gatgaatgtg aaccacgtca gccccctgga gttgaccccg cactgggagt
     7081 tcgaccccgc ccaattccat gaggtggtga acaaactcaa cgacctgacc aacggcgctg
     7141 gccctgaggc cggcggcgcg cgcattccgg ccttgacttc cagcgcgctt tctgccggga
     7201 tgggggagag gaaaccccgg cctgaaaccg ggttcacaga ccgcattcca gatgaccgcc
     7261 gtgccccttc attttctcgt cggaatcacc cgtttgattt ccatccactt tgccttgaag
     7321 agcctcgaca gcatcggcgc ggcggtggtg gcgtatggga tgcagatttc agtctcaccg
     7381 gaaatggctc ggcagtttcc gagtgtgtgg gcgctctatc tgccgtcttt ggcaatttac
     7441 ctgggcttgg tggttctcac atggttcgcg gctccgggca tttgcaggct tgccatgaga
     7501 tcgggcctac ctgtcgaaac acgcgaggac acttgcgaaa aggtggcctg gggtgagatc
     7561 atgatctttc tcaccggcat cctgctcatg tgctggggca tctcgcgggc ggcggcgggc
     7621 atcatgccca tcctgcaggc cgccagccgc gagcttcacc atgagcttgt cctggctgac
     7681 cagttggaat tcttcgtggc ctttgccctg acgggtgccg gcgggcttct tgccgcgcgt
     7741 tttccgagtg tttacaggtg gtgcgagaga cggaaaaggg aagccggctc atgaccggcc
     7801 cggtttgccg ctgccatcag gcggctgcgt ccccgtccgg ggcggccttg cggcgggcga
     7861 ggtggaaggg cagatgcagg aggctgtaaa ggacgaaggc gagaagtacg aggctgccga
     7921 tgtaccaggg ccaggggccg aggtggtcca tgaggctggc ctgctcgggc ttgcggcaga
     7981 ggaaggcgta gttggtgccg agggcggtgt tgatgaggcc ggtgagggcg gcgtagccga
     8041 gggtgaaggc gagcatgcgc cgcagcgcgc cgggccgcgg ggcgcgcccc atgctggtga
     8101 cgacgtggag ggcggtgacg acgacgccgc cgtggaggag aaagaagacg aggaagcgcg
     8161 ggtcggggaa gtcggccttt aaattcggtg tcagcagccc ctgcagggtg ccggcgaggc
     8221 cgaggtagta aacgacctcg caggcggtct gccggcgggt gagcagcgcc agcccggcgg
     8281 cgatgccggc aatgtcgcag tagtggagcg gcagggcgtt gccgagggag aggatgccgg
     8341 tgtgccagta ggccaggacg gtgaggggcc agatgagcag gagcaggaag cccagcacgc
     8401 gctcggcgcg cacggcctgg ctttcattca cccggcgcag aaatgccagc agcacgagag
     8461 cggccagaca ggctgccagg acggcgatgt ggctgcggct gaaagcttgg aaatcgtagg
     8521 gcggcatggc tttgaccgaa gattacccgg acgcgcgggg tgaatgcacg ccaaaaattc
     8581 accccggtct ggacgcggcg cgcgggctgt ggcttcatgc gccatgaacc cgcccgcgaa
     8641 tgtcgtcgaa ctctgccagg ccctggtgcg cattccctcg gtgaatccgg acggcgaccc
     8701 cggcacggcg cgcacgggcg aggcggagtg cgctcgtttc atcgcggctt tcctggagcg
     8761 ctgcggggcc gaggcttttc tcgacgaggt ggagcctgac aggccgaatg tgatcgggcg
     8821 cttcccttcc ctgccccgcc ccggcggcgg tgccaagccg cgcatcgtct tcggtccgca
     8881 cacggacacg gtgagcgtgg gcggcatgag catcgagcct ttcggcggcg agctgcggga
     8941 cgggcgcgtg tggggccggg gggcgagcga cacgaagggg ccgatggcgg cgatgctgtg
     9001 ggcgctgcat gagatgcgcg cgcgcatccc ggcgctgccg gtggaggtgc atttcgccgg
     9061 cttcatgtcg gaggagtccg cgcagctcgg ctcgcagcat ttcgccaggc atcacggctc
     9121 ctacgcgctc gctgtcgtgg gcgagccgac ggagttgcgc accgtcttcc gccacaaggg
     9181 ctgcctgtgg gcggacgtgg tcacgcacgg tgtggcggcg cacggggcca cgccggagcg
     9241 cggggagaat gccatcgtga aaatggcgcg cctcgtgcat gcgctggaca cggacttccg
     9301 cgcgctgctg gtggaggcgg gcggcgtgga cgagtggctc ggcgccagca ccatcaatct
     9361 gggcatgatc cagggcggcg cgcgcagcaa catcgtggcg gaccgctgcg cgctgcgcgt
     9421 ggacctgcgc accacgcccg gcctcaaccg cgcgggcggc gcgctgcgtc tgctggcgga
     9481 tttcatccgc aggctcgacc cgggcgcgga ggtgctgcct ctgccggaga ctttcccgct
     9541 ggacaccgac ccggaaaacc cgctcgtcca gaccctggtg aaatgcggcg cgcccctggc
     9601 cggcgcgccc tggttttgcg acgccgcttt tctcgccgcg gccggcatcc cggccatcgc
     9661 catcggcccc ggcagcatcg cgcaggcgca cacgaaggac gagttcatca gcgcggcgga
     9721 cctggaggct ggcgcggctt tttttgtccg gtttttgcag agtctggagg cggggtgttg
     9781 agtcaggcgc cgccacaaag ggtgagatcc gcccacaaag ggtgagatcc gccattggaa
     9841 tcatggagtg ctggagcatt ggaatcccat cactccagaa ctccagaatg ggcatcacca
     9901 atcgtgatga tgccggcata aatccggcgc gcgcgtgcca gcgcccgcct ttgctcaccg
     9961 cccgcgcttt ttcagcgccg ccagcacggc ggctttgtcg ccgcgaaggg cggcttccac
    10021 atcgagcacc aggcctggaa aaatccggct gcgcagccgt ccgtggcggt cgggcttcat
    10081 gcggatgtag gcgccggcct cgagttgaaa ccagtccacc tccttgtcct cggtgcgcca
    10141 gaccaggtat tcggccactt cgagacggcg atagacgcgc agtttgtcgc gcaaatctat
    10201 cgaaccggtg ctggccgcca cctccaccac catctcgggc gagccgcaca gataacccgc
    10261 ctcgttcacc cagaccctgc cgccccggcg cggcgtgccg cagaggatgg cgtcgggctg
    10321 gaagcagttc tcagtgtcca gcagcagggt cgtgttcggc agcatttcca ggccgtgctc
    10381 ggcggcgtag gcaaacaacc agccatgaat gattccatca ggctgcgagt ggttgaggcg
    10441 gacggcggat ggcatgtgga cgactccttc gatcagttcc gcttttttca ccccctgcat
    10501 tttctcgaaa cgacgaagaa actccggcag cgccaccagc tcaccgtttt caagcgggga
    10561 gctgtgcgcc tggcttcgac tggcgggtgc tggcaccgtg gcgggcatgg tttccaatac
    10621 actcgcgccg cttttttgtc atgtcctgct ctcaggtcct gacggtggag ggacggagcc
    10681 gggggcaggc ccgcccttga aatttccccg cattcacgcg ccataacacg ccttgccggc
    10741 gcgttcatcc agggcacttt ttcacacatg gactccagtc tccgccctgc cacccgccag
    10801 gcccttcaaa ctttccgcgc ccgccgccgc accctgctcg cctggcgcgc gctgctctgc
    10861 ttcgcgctca ttgtgctcgc ggcggcgctt ttcgccgcgc tgctggcccg cccgcgtttc
    10921 atgccggagg cgctgcg
//
LOCUS       DAOMYJ010000182        10814 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Prosthecobacter sp. isolate AMR_MDS_5629
            NODE_2031_length_10814_cov_23.012176, whole genome shotgun
            sequence.
ACCESSION   DAOMYJ010000182 DAOMYJ010000000
VERSION     DAOMYJ010000182.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867850
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prosthecobacter sp. (wastewater metagenome)
  ORGANISM  Prosthecobacter sp.
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Verrucomicrobiaceae; Prosthecobacter.
REFERENCE   1  (bases 1 to 10814)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10814)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.30
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:34:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,742
            CDSs (total)                      :: 4,684
            Genes (coding)                    :: 4,676
            CDSs (with protein)               :: 4,676
            Genes (RNA)                       :: 58
            tRNAs                             :: 55
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10814
                     /organism="Prosthecobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2031_length_10814_cov_23.012176"
                     /isolate="AMR_MDS_5629"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1965333"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            91..2859
                     /gene="fdhF"
                     /locus_tag="PLP58_16425"
     CDS             91..2859
                     /gene="fdhF"
                     /locus_tag="PLP58_16425"
                     /EC_number="1.17.1.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR01591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009326 - formate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0008863 - formate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0015942 - formate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase subunit alpha"
                     /protein_id="HRJ10165.1"
                     /translation="MVQDISTLTAFIDGEPHRFETGDTLLNFIDRHRGRGFVPTLCDA
                     PQLEPYGACRVCSVEVALQADGPRRVVASCHTPISAGMHIFTESQKVRRLRKNIVELV
                     LTDHPLDCLTCEVSGNCELQTVAAKVGIRGVRYPAGANHLDRAKDRSHPYMTSELSKC
                     INCSRCVRACDEVQGQHVLSMHGRGFNARIIKGLDQSFMDSTCVTCGACSQACPTSAI
                     SDVFFSKSIEATKKTRTVCTYCGVGCNLNVATKGDEVLSIQAPTDSEVNHSHTCLKGR
                     YAFKFVKHPERLRKPLIRYNGHFTEATWDEAYDYIAKNLKRIKEEHGGNAVGGISSAR
                     CTNEENYLMQKFIRVGFGTNNIDACARVCHSPTAWGMQKAFGTGAATNSIEDLEYTNC
                     ILITGANPTEGHPVTGSKIKQRVMKGVPLIVIDPRQIELVPYATHHLQLRPGTNVALL
                     DMFAYYILEAGLIDQDFIKKRCENWDEFEKGLRALDLDELERINGVPREKVRAAAIEY
                     ASAENAMAFHGLGVTEHSHGSKTVMTIADIAMMTGNIGRPGVGVCPLRGQNNVQGAAD
                     MGCQPHQGAGYLQVNDPGYHKLYEEFYGAHISNEIGWKIPQMYDAALRGDLKAMWIMG
                     EDVVQTDPNTEHVKNALNALEFLVVQELFMTPTCEFADVVLPASSFLEKSGTFTNGER
                     RVQRVNAVIPPIPGTKPDGVIMCDIMQRMGIPQDDYTPDGVLAEIARIVPFFQGATWE
                     NLTDQGTQWPIKEGGIGTEILHVDAFKRGLGKFHFFRFQESKEIEKHHDDFPFILTTG
                     RILEHYNCGTMTRRTGNSQIVSEDVLAIHPDDAKKKGIETGDLVRIFSARGEVSIKAK
                     VSTEVKPGILYTTFHFPEHLVNMVTSSECDEDTMCPEYKVVAVDVEKVSSPVAKKARK
                     SDLAPAMV"
     gene            3087..5702
                     /locus_tag="PLP58_16430"
     CDS             3087..5702
                     /locus_tag="PLP58_16430"
                     /inference="COORDINATES: protein motif:HMM:NF020390.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1800 family protein"
                     /protein_id="HRJ10166.1"
                     /translation="MPNSCLTIRAVILVLGCAAYATRALDSNSNQQSDIWEAFYNAHN
                     LPPGGDADLEGFSNFIECISGTNPFDAASFPRLGIIHGPDDGLSLHWSSQPGKSYGIL
                     GSPDLNPANFTLIGTAYGDGAEMLRELSANGQDRWFFKLEALDLDSDSDGLTDWEENA
                     IGFNPFLNHSDRHDTADLARVQSTLNAASVITAGLVDGDMREDWPDKGVIAIRRAGGL
                     GALTVNVTFTGTATRNVDYTASIPGTQVTLPLGAREAWIELAPVNDSEVEGLESITVT
                     VTAGTGYSLGTVTSDTAMLGDSSPLPSAKESARFLIQAAFGPDQDYPADGDDIPENVE
                     EVMGMGIATWIEDQFTRPLGYLQPYVDWAVENANAIQLYGNYKQHSWWSRAMGVPKLR
                     PDAVETQLPDLLRQRVAFALSEILVTSDRPESLAVDHRGMANYYDIFIRHAFGNYRDI
                     LHEVAMHPVMGVYLSHLGNQKANPVLMIHPDENFAREIMQLFSIGLWELNADGTRKTY
                     QAPHPYAGQFIPTYDNGDITELARVFTGLTFGDSIGFNPNNLTNGDRTQPMKMWDDYH
                     DCEAKTLLGGLQLPARTPSPGSTGAAGLADVEAAVIHLFNHPNVGPFIGRLLIQRFVT
                     SNPSHAYIGRVTAAFNDNGFGVRGDMKALIRAILLDPEARDPAMMELPYWGKMREPFL
                     RVVNLARAFNAASASGYYPLDQFVLDHAQDPMNSPSVFNFFLPGHSPPGPVTQMGLVA
                     PEFQILNASTAITGANYFYNAIGGNNLHRWGSGTAAYAVQLNLAPELSMVVPPAHINE
                     DTPSVANLLDTDTLIRRLDMSLLGGTMSPRLFQTIRESVDRIKPPDTSWRWHRERLRQ
                     LINHIVTSAEFNVMR"
     gene            complement(5721..6065)
                     /locus_tag="PLP58_16435"
     CDS             complement(5721..6065)
                     /locus_tag="PLP58_16435"
                     /inference="COORDINATES: protein motif:HMM:NF016876.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HRJ10167.1"
                     /translation="MSHILLLSSKARQHIREQCLWYRENLEDGVSLANRWTRTLDSAL
                     HQLCQHPERHPPASPEDQVLPGRQLRRMLFRPWNSGRGWKVLFCADEGARTVTVLYIR
                     HESRPPLEDDEP"
     gene            complement(6062..6298)
                     /locus_tag="PLP58_16440"
     CDS             complement(6062..6298)
                     /locus_tag="PLP58_16440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ10168.1"
                     /translation="MSTILQEQPAQYGASGDDELPRREAEALSKMDWEAYDKQSRSDL
                     AEAAKSFQEGELSMPAREFFDGLYLEDQAADSDA"
     gene            6362..7789
                     /locus_tag="PLP58_16445"
     CDS             6362..7789
                     /locus_tag="PLP58_16445"
                     /inference="COORDINATES: protein motif:HMM:NF019040.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1501 domain-containing protein"
                     /protein_id="HRJ10169.1"
                     /translation="MITSFSPNRRQFLGQASCAAVSSIPVLNTLLNLAVAEKVSAVTA
                     PADGEYRALVCIFLSGGNDSYNMLVPREASAYSAYQTSRSNLALAPGQLIDIHPVGLP
                     NFAVHSAMPEVAALFEAGNAAFVANVGTLIEPVQNRAQVTAMSKLLPLGLYSHSDQIE
                     QWQTSMPHQRSGIGWAGRVADILQDLNSNQAVPMNISLDGSNVWQTGDRVAEYSITPN
                     GAVALSNYKFGWQQYDTVSQAFSQGVDSQLAQQYSNVLMQTFNARKKSALEAYNSFAT
                     ASAPALPGGVSFPSSYLGSRLQMVARAIQGRNALGATRQTFFLNWGGWDHHSGVLGYQ
                     AAMLPQVSAAIGAFYQALVAMGVQNQVTLFTASDFGRTLTSNGQGSDHAWGGNQLVVG
                     GSVIGQRIYGQYPSLSVNPSSGPEVNPLDTGLGRMIPTTSCDEFFAELALWLGVAPGD
                     LPVILPNIENFYTPGGGAAPIGFMA"
     gene            complement(7796..9622)
                     /gene="aspS"
                     /locus_tag="PLP58_16450"
     CDS             complement(7796..9622)
                     /gene="aspS"
                     /locus_tag="PLP58_16450"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009961968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HRJ10170.1"
                     /translation="MIPNAYRSLHCNAIRAEHTGQNVTLCGWVNSARDHGGVIFIDLR
                     DREGLTQVVFRPEENSEVAAQSHHLRDEDVLQITGRVERRLAGTENARLPTGEVEVVA
                     TELTILNKADVLPFQLDRELSNEDLRMKYRYLDLRRERMNKNIRQRHKITNAARNFLD
                     GEGFVEVETPILSNPTPEGARDFLVPARLSPGKFFALPQAPQQYKQLLMVAGLERYFQ
                     IARCFRDEDLRADRQPEFTQIDIEASFVGQEDIIRLVEGLLAAMFKAGQGVDIPLPFP
                     RMTYREAMDRYGSDKPDTRFGLEITDMADVFAASEFKIFRATLENGGVVRAINAKSFA
                     GITTGQMIRLNELAIQAGMKVKQLAFIKVERDANGVLEYKSPLWKFFGEAEKAALIEK
                     LGVEENDIVFFYAGTWDEACNILGRVRLEIASMQQLTKDSTALNFLWVVDFPLLAFSP
                     EDQGWVAVHHPFTRPKSEDVPLLDAGEYAKVRAEAYDVVLNGYELGGGSIRIHESDLQ
                     AKMFGVLGVTSEEQELKFSHILEAFKFGAPPHGGLALGLDRIAMLVAGEESIREVIAF
                     PKNNKACDLMTDSPTTVDFKQLRELYVQSTWKEKKKDETAGA"
     gene            complement(9693..>10814)
                     /locus_tag="PLP58_16455"
     CDS             complement(9693..>10814)
                     /locus_tag="PLP58_16455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ10171.1"
                     /translation="QADIKAEEAGLRGLGADHLPRELEHARQALASAEAEAARRLKAL
                     DGMTDCRTPVSELRALLAQAEGVEKSARAARDAALRQHQQLEKQTADLAAGMRQAEAA
                     ADDLESRLRYLIEQHGEDAARAATLAESAGKAQAEEAVLEGLRTRLADLSPADLEADR
                     ARIQRSIQQQNQKRDSALQLHAGAASTLRADGGSDPVAALKTARSRLHAARARQETLA
                     LRSAALRRLHELYVQERQALSDQLTRPLAERVTGYLQRVFGPEAGILIAMDGATFSGL
                     ALGRGSAAAIGFDQLSGGAREQVAAAFRLAMAEILAQAHDGCLPVVFDDAFAYSDPER
                     VRRLQAMLDLAARRGLQIILLTCAPGDYALLGAREVTLA"
BASE COUNT         2074 a         3535 c         3236 g         1969 t
ORIGIN      
        1 ccccccccca tcgcgagaag actcgcgaac tacctcttcc caagcggcac cttcctgtgt
       61 aaaatgcgca aaactcaacg ttttccagcc atggtccagg atatctccac cctcaccgct
      121 ttcatcgatg gcgaaccgca tcgcttcgaa accggcgaca ccttgctcaa cttcatcgac
      181 cggcatcgcg ggcgcggctt tgtgccgacg ctgtgtgatg caccgcaatt ggagccttat
      241 ggcgcgtgcc gcgtgtgcag cgtggaggtg gctttgcagg ccgacgggcc gcgccgagtg
      301 gtggcgagct gccacacgcc gatctccgcg ggcatgcaca tcttcaccga gagccaaaaa
      361 gtgcgtcgtt tgcgcaaaaa catcgtcgaa ctcgtgctca cggaccatcc gctcgattgt
      421 ttgacctgcg aagtcagcgg gaactgtgaa ctccagaccg tggcggcgaa ggtgggcatt
      481 cgcggcgtgc gctatccggc gggggcgaat catctggacc gggcgaaaga ccgctcgcat
      541 ccctacatga cctccgaatt gtcgaagtgc atcaactgct cccgctgcgt gcgtgcctgc
      601 gatgaagtac aggggcagca tgtgctctcg atgcatggtc gcggcttcaa tgcgcgcatc
      661 atcaaggggc tcgatcaatc gttcatggac tcgacctgcg tgacgtgcgg cgcgtgctcg
      721 caggcctgcc cgacttcggc gatcagcgac gtgtttttct ccaaatccat cgaagcgacg
      781 aaaaaaacgc gcaccgtctg cacttactgc ggcgtgggct gcaatctcaa cgtggcgacg
      841 aagggcgatg aagtgctctc catccaggcg ccgacggatt ccgaggtgaa tcacagccac
      901 acctgcctca aaggccgcta cgcgttcaag ttcgtgaagc accccgagcg cctgcgcaag
      961 ccgctcatcc gctacaacgg ccatttcacc gaggcgacgt gggacgaggc ttatgattac
     1021 atcgcgaaga atctgaagcg catcaaagag gagcacggtg gcaatgcagt gggcggcatt
     1081 tcctccgcgc gttgcacgaa tgaggagaac tacctcatgc aaaaattcat ccgtgtgggc
     1141 ttcggcacaa acaacatcga cgcatgcgcc cgcgtgtgcc attcgccgac cgcctggggc
     1201 atgcagaagg cctttggcac cggcgcggcc acgaacagca tcgaggacct cgaatacacc
     1261 aactgcatcc tcatcaccgg ggcaaacccg acggaaggcc atcccgtgac cggttcaaag
     1321 atcaagcagc gcgtgatgaa aggcgtgccg ctcatcgtga tcgacccgcg ccagatcgag
     1381 ctcgtgccct acgccacgca tcacctccag ctccgccccg gcacgaacgt ggcgctgctc
     1441 gacatgttcg cgtattacat cctggaggcc ggcttgatcg accaggactt catcaaaaag
     1501 cgctgcgaga actgggacga gttcgagaaa ggcctgcgcg ccctcgattt ggatgagttg
     1561 gagcgcatta acggcgtgcc gcgtgaaaaa gtccgtgccg ccgccatcga atacgccagc
     1621 gcggagaacg caatggcctt ccacggtctc ggcgtcaccg agcacagcca cggctcgaag
     1681 accgtgatga ccatcgccga catcgccatg atgaccggca acatcggccg tcccggcgtg
     1741 ggcgtgtgtc cgttgcgcgg ccagaacaac gtgcagggcg ccgctgacat gggctgccag
     1801 ccgcatcagg gcgctggcta cctccaggtg aacgatcctg gctaccacaa gctctacgag
     1861 gagttttacg gcgcgcacat cagcaacgag atcggctgga aaatcccgca gatgtatgac
     1921 gccgcgttgc gtggcgatct gaaagccatg tggatcatgg gcgaggacgt ggtgcagacc
     1981 gatccaaaca ccgagcacgt caaaaacgcg ctcaacgccc tcgaattcct cgtcgtgcag
     2041 gagctgttca tgacgcccac ctgcgagttc gccgatgtcg tgctacccgc atcgtcgttc
     2101 ctcgaaaaaa gcggcacttt caccaatggc gaacgccgcg tgcagcgcgt gaacgccgtc
     2161 ataccgccga tccccggcac aaagcccgat ggcgtcatca tgtgcgacat catgcagcgc
     2221 atgggcatcc cgcaggacga ttacacgccg gatggagtgc tggcggagat cgcgcgcatc
     2281 gtgcccttct tccaaggcgc gacctgggag aacctcaccg accagggcac gcaatggccc
     2341 atcaaggaag gcggcatcgg caccgagatc ctgcatgtgg acgcgttcaa acgcggcctc
     2401 ggcaagttcc acttcttccg cttccaggaa tcgaaggaga tcgagaagca ccacgacgac
     2461 ttccccttca tcctcactac cggccgcatc ctggagcact acaactgcgg caccatgacc
     2521 cgccgcaccg gcaattcgca gatcgtcagc gaggacgtgc tcgccatcca tcccgacgac
     2581 gcgaagaaga agggcatcga gacaggcgac ctcgtccgca tcttctccgc ccgtggcgag
     2641 gtgagcatca aagccaaagt ctccaccgag gtgaagcccg gcatcctcta caccaccttc
     2701 cacttccccg agcacctcgt gaacatggtc accagcagtg aatgcgacga ggacaccatg
     2761 tgccctgagt ataaagtcgt ggccgtggat gtggaaaaag tgtcctcgcc tgtggctaaa
     2821 aaagcacgca agagcgatct cgctccggcg atggtgtgat cgggatacgc gacgctttgt
     2881 cgcttatgaa gatgccccgg cccgcatcgc cacttacctg aaccagcacg cggcgcttct
     2941 gcggcgcagg cgtaggatta ggacgggcac gaactgcgcg tgcattgccc aggaaagcgc
     3001 ttctgattca tcaccgcagg catcactcca aaggattatt tccttttttg cgaattgtcg
     3061 aaaagtggca gtcaacgatc cccgccatgc caaactcttg cctcacaatc cgcgcggtca
     3121 tccttgtcct tggctgcgcc gcttacgcca cgcgagcgct cgattcgaac tccaatcagc
     3181 agagcgacat ctgggaggca ttttataacg cgcacaacct cccgcctgga ggtgacgccg
     3241 acctcgaagg cttctccaat ttcatcgaat gcatctccgg caccaacccg ttcgacgccg
     3301 cctcttttcc ccgactcggc atcatccacg gcccggatga cggactcagt cttcactggt
     3361 catcgcagcc aggcaagagc tacgggattc tcggcagccc ggatttgaac ccggccaatt
     3421 tcaccttgat cggcacagcc tacggcgacg gtgccgagat gctgcgggag ctgtcggcca
     3481 acgggcagga ccgctggttt ttcaaactcg aagcgcttga tttggacagc gacagcgacg
     3541 gtctcacaga ctgggaggag aacgccatcg ggttcaatcc cttcctcaat cacagcgacc
     3601 gtcacgacac cgccgacctg gcgcgcgtgc agagcacgct caacgcggcc agcgtcatca
     3661 ccgccggtct tgttgacggc gacatgcgcg aggactggcc ggacaagggc gtcatcgcca
     3721 tccggcgcgc gggcggcctc ggcgcgttga cggtgaatgt caccttcacc ggcacggcca
     3781 cgcggaatgt ggattacacc gccagcatcc caggcacaca agtcacacta cctctcggcg
     3841 cgcgcgaggc atggatcgaa ctggcgccgg tcaatgactc agaggttgag gggttggaaa
     3901 gcatcacggt cacggtgaca gcaggcacag gctacagtct cggcaccgtc accagcgaca
     3961 ccgccatgct gggcgactcg tcgccgctgc cctcggcaaa ggagtccgcg cgttttctca
     4021 tccaggcggc ctttggcccc gaccaggatt atccagcgga tggagatgac atcccggaaa
     4081 atgtcgagga ggtcatgggc atgggcatcg cgacctggat tgaggatcag ttcacgcgcc
     4141 cgctcggtta tttgcagccc tatgtggact gggcggtgga aaatgcgaat gccatccagc
     4201 tctacggcaa ctacaagcag cactcctggt ggtcgcgcgc catgggcgtt ccgaagctgc
     4261 ggccggatgc cgtggagacg cagctgcccg acctgctgcg ccagcgcgtg gcctttgccc
     4321 tcagcgagat cctcgtcacc tccgacaggc ccgagtcgct cgccgtggat catcgcggca
     4381 tggcgaacta ctacgacatc ttcatccgcc acgccttcgg caactaccgc gacatcctcc
     4441 atgaggtcgc catgcatccg gtcatggggg tgtacctgag ccatctgggc aatcaaaagg
     4501 ccaacccggt actgatgatc cacccggatg aaaacttcgc gcgcgagatc atgcagcttt
     4561 tcagcatcgg cctgtgggag ctgaatgccg acggcacgcg caaaacctat caggcgccgc
     4621 atccttacgc gggccagttc atccccacct atgacaatgg cgacatcacc gaacttgcgc
     4681 gcgtcttcac cggcctgact tttggcgaca gcatcggctt caatcccaac aacctcacca
     4741 acggcgaccg cacccagccg atgaagatgt gggacgacta ccacgactgc gaggccaaga
     4801 ccctgctcgg cggcctgcaa ctgcccgcgc gcacccccag ccccggcagc accggcgcgg
     4861 cggggctggc ggatgtggag gcggcggtga tccatctttt caatcatccc aacgttggcc
     4921 ccttcatcgg tcggctgctc atccagcgct tcgtcacctc gaatcctagc catgcataca
     4981 tcggacgcgt gacggctgct tttaacgaca atggattcgg cgtgcgtggc gacatgaagg
     5041 cgctgatccg ggccatcctg ctcgacccgg aggcgcgtga tccggcgatg atggaactgc
     5101 cctattgggg caaaatgcgc gagcctttcc tgcgtgtcgt caatctcgcc cgcgccttca
     5161 acgccgcctc cgcctcgggc tactatcctc tcgaccagtt cgtgctcgac cacgcgcagg
     5221 acccgatgaa ttcgcccagc gtcttcaact ttttcctgcc cggccacagc ccgcccggac
     5281 cggtcacgca gatggggctg gtggcgccgg agtttcagat tctcaatgcc agcaccgcca
     5341 tcaccggagc caactacttt tacaatgcca tcggcggcaa caacctgcac cgctggggca
     5401 gcggaaccgc cgcctacgct gtgcaactca atctcgcgcc cgagctgtcc atggtggtgc
     5461 cccccgccca catcaacgag gacacgccca gcgtcgccaa cctgctcgat accgacacgc
     5521 tcatccgccg gctcgacatg agcttgctgg gcggtaccat gagcccgcgc ctctttcaaa
     5581 ccatccgcga gtccgtagac cgcatcaaac caccggacac ctcctggaga tggcaccgcg
     5641 aacgcctgcg ccagctcatc aaccacatcg tcacctcggc cgagttcaac gtgatgcgct
     5701 gagtccccgc cgccccaacc tcatggttca tcatcctcca gcggcggacg gctttcgtgc
     5761 cggatgtata ggacggtcac cgtcctggcc ccctcatcgg cgcagaacag cactttccag
     5821 ccgcgcccgc tattccaggg gcggaacagc atgcgtcgga gctgccgtcc aggcagcacc
     5881 tggtcttctg gtgaagcggg aggatggcgt tcgggatgct ggcagagttg atgaagcgcg
     5941 gaatccagtg tgcgagtcca gcggtttgcc agagacacgc catcctcaag attctcgcga
     6001 taccacagac actgctcgcg aatgtgctgc ctggctttcg aggaaagaag cagtatatga
     6061 ctcatgcgtc ggagtccgcc gcctgatcct caagataaag gccgtcaaaa aactcgcgcg
     6121 ctggcatgct caattcccct tcttgaaagc ttttggctgc ctccgccaga tcgctcctcg
     6181 actgcttgtc gtaagcctcc caatccattt tggacaaagc ttccgcctcg cggcgcggca
     6241 actcgtcatc gccggaagcg ccatactgcg cgggctgttc ctggaggatc gtgctcatgc
     6301 gcgcagcata gcatgacgct gccattgccc gcaaacctca ttttcacacc ctccccactc
     6361 aatgatcacc tccttctctc cgaaccgccg ccagttcctc ggccaggcaa gctgtgctgc
     6421 cgtgagctcc atcccggtgc tgaacacgct gctgaatctc gccgtggcgg aaaaggtttc
     6481 cgcggtcacg gcgccggccg atggcgaata ccgcgcgctg gtctgcatct tcctctccgg
     6541 tgggaatgac agctacaaca tgctcgtgcc gcgcgaggct tcggcctatt cagcatacca
     6601 aacaagccgg tcgaatctgg cgctggctcc agggcagttg attgacattc atccggtcgg
     6661 tcttccgaac ttcgcggtgc attcggcaat gcctgaggtg gcggctttgt ttgaggcggg
     6721 caatgccgcc tttgtcgcca acgtcggtac gctgatcgaa ccggtgcaaa accgcgcgca
     6781 ggtcacggcg atgagcaagc tgctgccgct cggcctgtat tcgcattccg accagatcga
     6841 gcaatggcag accagcatgc cgcaccagcg cagcggcatc ggctgggccg ggcgagtggc
     6901 ggacatcctg caggacctca attccaacca ggccgtgccg atgaacatct ccctcgacgg
     6961 cagcaacgtc tggcagacgg gcgatcgcgt ggcggagtac tcgatcaccc cgaacggcgc
     7021 ggtggcttta tcgaactaca agtttggctg gcagcagtat gacacggtgt cacaggcatt
     7081 cagccagggc gtggacagcc agctcgcgca gcagtacagc aacgtgctca tgcagacctt
     7141 caacgcgcgc aagaagtcgg ctctcgaagc ctacaacagc ttcgccactg ccagcgcgcc
     7201 cgccctgccc ggcggcgtgt cttttccctc cagctacctc ggctcgcgcc tgcaaatggt
     7261 ggcgcgcgcc atccaggggc ggaacgcgct cggcgccacg cggcagactt tctttttgaa
     7321 ctggggtggc tgggatcatc acagcggtgt gctcggctac caggctgcga tgctgccgca
     7381 ggtcagcgcg gcgattggcg cgttttacca ggcgctggtg gcgatgggtg tgcaaaacca
     7441 ggtcaccctc ttcaccgcct ccgacttcgg ccgcacgctc accagcaacg gccagggcag
     7501 cgaccacgcc tggggcggca accagctcgt ggtcggcggc tcggtcattg gccagcgcat
     7561 ctacgggcag tatcccagcc tctccgtgaa cccctcgtcc ggtcccgagg tgaacccgct
     7621 cgacaccggc cttggccgca tgatcccgac aacaagctgt gacgaattct tcgccgaact
     7681 cgcgctctgg ctcggagtcg cccccggtga tctgccggtg atcctgccga acatcgagaa
     7741 cttttacaca cccggcggtg gcgcggcacc catcgggttc atggcatgac gcgcgtcacg
     7801 cgccggcggt ttcgtccttc tttttttcct tccaggtgct ttgcacatac aactcgcgca
     7861 gttgtttgaa gtccaccgtg gtcggactgt cggtcatcaa atcgcaggct ttgttgtttt
     7921 tcgggaaggc gatgacctcg cggatgctct cctcacccgc caccagcatg gcgatacggt
     7981 cgagaccaag cgccaggccg ccgtgcggag gcgcgccgaa tttgaaggcc tccaggatgt
     8041 ggctgaactt aagttcctgc tcctctgaag tcactccgag cactccgaac atcttcgcct
     8101 gaagatcgct ctcatgaatt cgaatggagc cgccgccgag ttcgtagccg ttgagcacca
     8161 cgtcgtaggc ctcggcgcgg actttggcgt attcgccggc atccagcagc ggcacgtcct
     8221 cagacttggg gcgggtgaag gggtgatgca cggccaccca gccctggtct tcggggctga
     8281 aggcaagcag tgggaaatcc acgacccaga ggaaattcaa cgcggtgctg tccttcgtga
     8341 gctgctgcat ggacgcgatc tccaggcgca cgcgaccgag gatgttgcat gcctcgtccc
     8401 aggtgccggc atagaagaag acgatgtcat tttcctcgac accgagcttt tcaatgaggg
     8461 cagctttttc ggcctcgccg aaaaacttcc acagcgggct tttgtattcg agcacaccgt
     8521 tggcgtcacg ctccactttg atgaaagcga gctgcttcac cttcatgccg gcctggatgg
     8581 ccagctcgtt caggcggatc atctgcccgg tggtgatgcc ggcgaagctc ttggcgttga
     8641 tggcgcgcac gacgccgccg ttctccagag tggctcggaa gattttgaac tcggacgcgg
     8701 cgaagacatc cgccatgtcg gtgatttcca gtccaaagcg ggtgtcaggc ttgtcggagc
     8761 catagcggtc catcgcctca cggtaggtca tgcgcggaaa tggcagcggg atgtccacgc
     8821 cctggcccgc tttgaacatg gccgccagga ggccctcgac caggcggatg atgtcctcct
     8881 gcccgacaaa gctggcctca atgtcaatct gggtgaactc cggctggcgg tcggcgcgca
     8941 gatcctcgtc gcggaagcag cgggcgatct ggaagtaacg ctccagtccg gccaccatga
     9001 ggagctgctt gtactgctgc ggcgcctgcg gcagggcgaa aaatttgccg gggctgagac
     9061 gcgcgggcac aaggaagtcg cgcgctcctt ccggggtcgg gttggaaagg atgggggtct
     9121 ccacttccac gaagccctca ccatcgagga agttgcgggc ggcgttggtt atcttgtgac
     9181 gctggcggat gttcttgttc atgcgctcgc ggcgcaggtc caggtagcgg tacttcatcc
     9241 gcagatcctc gttcgaaagc tccctgtcga gctggaaggg cagcacatcg gccttgttga
     9301 ggatggtcag ctctgtggcc acgacctcca cttcaccggt gggcagcctg gcgttttccg
     9361 tgcctgcgag gcgccgctcc actcggccgg tgatttgcag cacatcctca tcgcgcaaat
     9421 ggtggctttg cgcggccacc tcgctgtttt cctccgggcg gaagaccacc tgggtgaggc
     9481 cctcgcggtc gcgcaggtca atgaagatga ctccgccgtg gtcgcgcgcg gaattcaccc
     9541 agccgcacag cgtgacgttc tggccggtgt gttcggcgcg gatggcgttg cagtggagcg
     9601 agcggtaggc gttcgggatc atggtgaaaa aggggcgggg agaatcgggg aaaaccgctg
     9661 gagtgcaagg gcggatgcaa cccgcgcgct cctcaagcca gcgtcacttc ccgggcaccg
     9721 agcagcgcgt aatcaccggg cgcacaggtg agcagaatga tctgcagacc gcggcgagcg
     9781 gcgaggtcga gcatggcttg cagcctgcgc actcgctcgg gatccgaata ggcgaaggcg
     9841 tcgtcaaaca ccaccggcag gcagccatcg tgggcctgag ccaggatttc tgccatcgcc
     9901 agccggaagg cggccgccac ctgctccctg gcgccgccgc tgagctggtc gaaaccgatg
     9961 gccgcggcgc tgccgcggcc gagcgcgagg ccggagaaag tcgcgccatc catcgcgatc
    10021 aggatgccag cctcagggcc aaagacacgc tggagatagc cggtgacgcg ctccgccagc
    10081 gggcgggtga gctggtcgga aagcgcctgg cgctcctgaa catacaactc gtgcaggcgg
    10141 cggagcgcgg cggagcgcaa agccagagtc tcttgcctgg cacgggccgc gtgcaggcgc
    10201 gaccgggcgg ttttcaaagc ggccacggga tcgctgccac catcggcgcg gagagtggat
    10261 gccgcgccgg catggagttg cagggcggaa tcgcgctttt gattttgctg ctggatggag
    10321 cgctggatgc gcgcgcggtc ggcctccaga tcggctggtg aaagatccgc gagccgggtg
    10381 cgcaacccct ccaggacagc ctcctccgcc tgtgcttttc cagctgattc ggcaagagtg
    10441 gcggcgcgcg cggcatcctc tccgtgctgc tcgatgagat agcgcaggcg gctttccaaa
    10501 tcatccgcgg cggcctcagc ctggcgcatc ccggcggcga gatcggcggt ttgtttttcc
    10561 aactgctgat gctggcgcaa agcggcgtca cgcgcggcgc gggccgactt ctccacgccc
    10621 tcggcctgtg ccagaagggc gcgcagctca gagaccggcg tgcggcaatc agtcatgcca
    10681 tccagcgcct tgagacggcg cgccgcctcg gcctccgcgc tggcaagcgc ctgccgcgca
    10741 tgctccagct cgcgcggcaa atggtcagcg ccgagtcctc gcagcccggc ctcctcagcc
    10801 ttgatgtcgg cctg
//
LOCUS       DAOMYJ010000183        10741 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Prosthecobacter sp. isolate AMR_MDS_5629
            NODE_2043_length_10741_cov_21.231799, whole genome shotgun
            sequence.
ACCESSION   DAOMYJ010000183 DAOMYJ010000000
VERSION     DAOMYJ010000183.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867850
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prosthecobacter sp. (wastewater metagenome)
  ORGANISM  Prosthecobacter sp.
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Verrucomicrobiaceae; Prosthecobacter.
REFERENCE   1  (bases 1 to 10741)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10741)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.30
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:34:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,742
            CDSs (total)                      :: 4,684
            Genes (coding)                    :: 4,676
            CDSs (with protein)               :: 4,676
            Genes (RNA)                       :: 58
            tRNAs                             :: 55
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10741
                     /organism="Prosthecobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2043_length_10741_cov_21.231799"
                     /isolate="AMR_MDS_5629"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1965333"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..777
                     /locus_tag="PLP58_16460"
     CDS             <1..777
                     /locus_tag="PLP58_16460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ10172.1"
                     /translation="DGGPVKLRRLPPRRPELERKPEPWPTLRPGTAEERAALARLRGF
                     SLGGIELAERRGLLHFGTYDAACPWRQHWRGAAFWAVTDAARRLVELRRLDGAFWPER
                     DGGTPRRKAHSIGTGKDRPCGIEEAAGFSFIGICEGMPDLVAAHDLAWQLGAAERAGF
                     CTVLGAGVRRLAAECLPCFKGRRVRLYPHCDESGGDAARAWALQIKEAGALVVDAFDL
                     SGLTTKDGKPGKDLADLVNADPRCLRGNPELLEDLFPCNQ"
     gene            774..1937
                     /locus_tag="PLP58_16465"
     CDS             774..1937
                     /locus_tag="PLP58_16465"
                     /inference="COORDINATES: protein motif:HMM:NF024872.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HRJ10173.1"
                     /translation="MNEPKTARKTRVMDPTCPQAGGTKAEKAREPEKPLFRVWTLGEL
                     RRLEEPEGWNLVGDYHITRGAVTVLAGSPGIGKSFASLMLGIQAAQGTGEWLGYKINR
                     PFKTLIIQAENNLLRLIMDAKRVMMPEEHNERLKVLEFDWGNISLQDLKLLTKLRELV
                     ADFQPDLVVVDPFNQFSGDSNEKECKEAVRGILSILEAAPVPPACLIVAHNRKKREGD
                     NHAGRRLADLISGSYVMQSNPRCVISYLPYSDDPEDNRVVVVTVKKNNGKSKGPATAW
                     RLLEHGFEKLDDFDFEEWSGGGNAGPKKDKSKIRLEHLQEVFRGGALTRQELGRKLEE
                     LTGAGRSAVSEAVNKRFAHCLEKLPGGLLFTLKEEHKAESSPFEDDEGDGGED"
     gene            2110..2427
                     /locus_tag="PLP58_16470"
     CDS             2110..2427
                     /locus_tag="PLP58_16470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ10174.1"
                     /translation="MPTAAAGQIVLAKPEIFGTGWVFMRGFGCLPLMAAVGFGLWLKW
                     EWWIWVIVVLVAWVLGGSLKRAFCKTAVDDVVRHAMKDPAFYDQLLLNRILRVEAKME
                     AGQ"
     gene            2643..3290
                     /locus_tag="PLP58_16475"
     CDS             2643..3290
                     /locus_tag="PLP58_16475"
                     /inference="COORDINATES: protein motif:HMM:NF012464.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HRJ10175.1"
                     /translation="MKTASSHVPVAILVRVSTSKQETARQVSELKALADSKGWQVAAI
                     CQESGVSGRAAAEDRHGLQEALALAKAGKIKKVLVHEVSRLGRRPSVVMSFVEALEAC
                     CVSLYWHAQRIETLLPNCKRNPAAGIMLALLAEMARAEVETMRERINSGLAEARRKGV
                     TLGRPVGSGLDAPALLAKHKDVVKALKAGHSVRNAAKITGKGPSTVQRVKAALEA"
     gene            complement(3964..4503)
                     /locus_tag="PLP58_16480"
     CDS             complement(3964..4503)
                     /locus_tag="PLP58_16480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ10176.1"
                     /translation="MARLDVESGRVRLEITADVAGNPLIQDESAAEEAVRTSLQLRTP
                     AGLVPLSGLGSMTLERRSEWDAGAPALSAPATPGQPHQLTTAVWEWRPDLPDLVFAVP
                     RGSLHDVLLWTRAGASTGKQAQWTLLVEGESSPVIQIPRTASGKMLAWLSSGLVLAGV
                     LLLHHNRSRFRRIRAFSLE"
     gene            complement(4593..5555)
                     /locus_tag="PLP58_16485"
     CDS             complement(4593..5555)
                     /locus_tag="PLP58_16485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ10177.1"
                     /translation="MFPRSTLLFLLALGTASGEVTYEFQPDFIQPPPGRETIGNGHGE
                     IACDSAGNFYVSVQGPENKDAGVQVYGPDGKFVRALNLPNSLHGFVIRKDEGREFIFA
                     AVLGEKKVIKASLDGAIVLEIPTSAFPEGKAGPKGLALTNCDVAPNGDIYVVDGYGQS
                     WIFVFDRAGKFKKVFGGPVEPLKLANTHKIFIDRRFEPARVLACDRGNNRLLHLDLDG
                     NLISVIASEGLRRPSSASFHGDLMCVAEIAGRVSVWDKEGILVAQLGTNDTPGQTNTP
                     KVEPKDWLQGTVTSPHGITFDNQGNILETEWNLFGRVLRWNRKG"
     gene            5703..5792
                     /locus_tag="PLP58_16490"
     tRNA            5703..5792
                     /locus_tag="PLP58_16490"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5737..5739,aa:Ser,seq:cga)
     gene            complement(5805..6197)
                     /locus_tag="PLP58_16495"
     CDS             complement(5805..6197)
                     /locus_tag="PLP58_16495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ10178.1"
                     /translation="MPALSNDAARCEVMNLGWGPNGHGPYLVRQEGYEPGSSTFKMQR
                     FILKRDGRWLLNLAFVMLPEAEQEAQLFHSLKDVLVLLDQLAGQPVLADAALPPGTNA
                     DEILEHFEQCTHRILRGMRDAAATPLHS"
     gene            complement(6201..6722)
                     /locus_tag="PLP58_16500"
     CDS             complement(6201..6722)
                     /locus_tag="PLP58_16500"
                     /inference="COORDINATES: protein motif:HMM:NF025066.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE/DsrF/DrsH-like family protein"
                     /protein_id="HRJ10179.1"
                     /translation="MSDDKQPVKKMSIIVSRGSLDGIYPALIMANGARMEGIEASLFF
                     TFFGLNALANKTLDSLKVSTVGNSALNMAMPMLGLPMTMPFPTWMGAIPGVSAFATRL
                     MNKEMEKLDIPPVREFLELISDSGGKIYACKLAMDMFKLKKEDLMERVDGVLTVGDFY
                     NESAGAGTHILFT"
     gene            complement(6752..7063)
                     /locus_tag="PLP58_16505"
     CDS             complement(6752..7063)
                     /locus_tag="PLP58_16505"
                     /inference="COORDINATES: protein motif:HMM:TIGR03342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TusE/DsrC/DsvC family sulfur relay protein"
                     /protein_id="HRJ10180.1"
                     /translation="MKKNIAGTEIDVNEEGYLTDMSQWNEAIAAAIAVEEGIGALGDG
                     HMKVVRYLREQQAKGAALTIRSMGKSGVVTTKELYDLFPGGPLKKSSKIAGIPKPVGC
                     I"
     gene            complement(7070..8299)
                     /locus_tag="PLP58_16510"
     CDS             complement(7070..8299)
                     /locus_tag="PLP58_16510"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF019604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD/NAD(P)-binding oxidoreductase"
                     /protein_id="HRJ10181.1"
                     /translation="MNKHLLILGGGTAGTMMANHLRRLLSATEWRMTVVDRSEKHLYQ
                     PGFLFLPFGIYDESQIVRQTRDFMPHGVEFMQAEVDQITSQDHAVTLKDGRELKYDVL
                     IVATGCRTAPEETEGMLGPKWRVNVHDFYTLDGAKALREKLQSFPGGRVVVHINEMPI
                     KCPVAPLEFTFLADTFFRERGIREKVTLTYVTPLSGAFTKPKASKKLGHLLTDKRIEL
                     VTDFVAERVDQENDKLVCFDGREVPYDLLVTTPTNMGDEAVRRSSLGDDLDFIPTHKH
                     TLQSLQHPDVFVIGDATNVPASKAGSVAHFESETLADNVMRHIQGEPLKEEFDGHSNC
                     FIESGNGKALLIDFNYEYEPHEGNFPFAFGPMKLLEESRLNHFGKLAFRHVYWNVLLK
                     AWPMPGISRQKKKPLNA"
     gene            8383..8844
                     /gene="trxA"
                     /locus_tag="PLP58_16515"
     CDS             8383..8844
                     /gene="trxA"
                     /locus_tag="PLP58_16515"
                     /inference="COORDINATES: protein motif:HMM:TIGR01068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HRJ10182.1"
                     /translation="MSASLESDARGIILPCPACGTANRIAYAKVSQQGRCGTCKADLP
                     LVAAPAEISDIEAFTALVSQSPLPVVIDFWAPWCGPCQMMAPEFAKAAAQAAGEALFV
                     KVNTDEQPQIAGQFRIQGIPAFALIRDGKLTAQTSGFQPAARLLAWMRQAN"
     gene            8928..10202
                     /locus_tag="PLP58_16520"
     CDS             8928..10202
                     /locus_tag="PLP58_16520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009961375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="neutral/alkaline non-lysosomal ceramidase
                     N-terminal domain-containing protein"
                     /protein_id="HRJ10183.1"
                     /translation="MPFILAGLILAQTAPAPKSVAPHVPAVWKAGLASVIVTPEKLIW
                     MAGYAARKKPAEGKQQELFAKALALEDEAGARMVFVTLDLIGVPQSLRRRLAAAVEKE
                     HGLPPSALLMNASHTHSGPQLRGSPVTDADLENPKIRDAWEYTQDLEGKILGIIRAAL
                     GDMKPARLTWNKARCGFAMNRRRDYTLPPEHPNANKAPNPAGVVDHEVPSLRIESPEG
                     APRGVLFGYACHNTCLGFYNWCGDYAGYAQEYLEKHRPDFQAMFLTGCGGDQNPYPRR
                     SEVVPGVSDVELAQQHGRSLANAVEVAISANPMPVTGRLRSAYEEVTLHYARDGKEPH
                     AYPVQVVKFGDDLTLVALGSEVVVDYSLRLKKELAGKAGVWVAGYSNDYTGYIPSLRI
                     QKEGGYEAAAGWAEDVEERIISKVHELHKSLP"
     gene            10484..>10741
                     /locus_tag="PLP58_16525"
     CDS             10484..>10741
                     /locus_tag="PLP58_16525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ10184.1"
                     /translation="MDPAAVAKDKTRRDQASGMITLTETLREYLRNINFLSNADLFNY
                     CNMIHANARERARRGVPGADTIFADLQAHFPGRRRASTPAPP"
BASE COUNT         2065 a         3030 c         3597 g         2049 t
ORIGIN      
        1 gacggcgggc ctgtgaagct gcggcggctg cctccccggc ggcctgagct ggagcgtaag
       61 ccggaaccct ggccgactct gcggccaggg acggcggagg agcgggcggc actggcgcgg
      121 ctgcggggct tcagcctggg cggcattgag ctggcggaac ggcgcgggct gctgcatttc
      181 gggacgtatg acgcggcctg cccttggcgg cagcactggc gcggcgcggc tttttgggcg
      241 gtgacggatg cggcgcggcg gctggtggag ctgcggcggc tggacggcgc tttctggccg
      301 gagcgggacg gcggcacgcc acggcgcaag gcgcatagca tcggcacggg gaaggaccgg
      361 ccctgcggca ttgaggaggc ggcgggcttc tccttcatcg gcatctgtga aggaatgccg
      421 gacctggtgg cggcccatga tctggcgtgg caactgggcg ctgcggaacg ggcgggcttt
      481 tgcacggtgc tgggcgcggg tgtgcggcgg ctggcggcgg agtgcctgcc ctgcttcaag
      541 gggcggcggg tgcggctgta tccgcactgc gatgaaagcg gcggcgatgc ggcgcgggcc
      601 tgggcgctgc aaatcaaaga ggcgggtgcg ctggtggtgg atgccttcga cttgagcgga
      661 ctgaccacga aggacgggaa gccaggcaaa gacctggcgg acctggtgaa cgcggacccg
      721 cgctgcctgc ggggcaaccc tgaactgttg gaggatcttt ttccatgcaa tcaatgaacg
      781 aaccgaagac cgcacggaaa actcgggtga tggacccgac ctgcccgcaa gcgggcggaa
      841 cgaaagcgga gaaagccagg gagccggaga agccgctttt ccgcgtgtgg accctggggg
      901 aactgcgacg gctggaggag ccggagggct ggaatctggt gggggactac catatcacac
      961 gcggcgcggt cacggtgctg gccggaagtc ctggcattgg caagagcttt gcctcgctga
     1021 tgctgggcat ccaggcggcg caaggcacgg gggaatggct gggctacaag atcaaccggc
     1081 cattcaagac gctgatcatt caggcggaaa acaacctgct gcggctgatc atggatgcca
     1141 agcgggtgat gatgccggag gagcacaacg agcggctgaa ggtgctggaa ttcgactggg
     1201 gcaatatcag cctgcaagac ctgaagctgt tgacgaagct gcgggagctg gtggcggact
     1261 tccagccgga cttggtggtg gtggacccgt tcaaccagtt ttccggggac tccaacgaga
     1321 aggaatgtaa ggaggcggtg cggggcattc tgtccattct ggaagccgcg ccggtgcctc
     1381 cggcctgcct gatcgtggcg cacaaccgga agaagcggga gggtgacaac catgccgggc
     1441 ggcggctggc ggacctgatc tccggcagct atgtgatgca gagcaacccg cgctgtgtga
     1501 tctcctacct gccctattcc gacgatcccg aagacaaccg cgtggtggtg gtgacggtga
     1561 agaagaacaa cgggaaaagc aaaggcccgg ccacggcctg gaggctgctg gagcatggct
     1621 ttgaaaagct ggatgacttc gactttgaag aatggagtgg cggcggcaat gccgggccga
     1681 agaaggacaa gagcaagatt cgcctggagc acttgcagga ggtcttcaga ggcggggcgc
     1741 tgacacggca ggagctgggc cggaagctgg aagaactgac cggcgcgggc cggagcgcgg
     1801 tttccgaagc ggtgaacaag cgctttgcgc actgcctgga gaagctgccg ggcgggctgc
     1861 tgttcacgct caaagaggag cacaaagcgg agtcctcgcc tttcgaggat gacgaagggg
     1921 acggcgggga ggattgagcg ggcggcgggt tccggctcct cctccgggtg aatctctaaa
     1981 ggggaggagc cagaggagga attcattttg cttgtgccaa tgaaaaggcg agtttgaaaa
     2041 atggagccgt tattacttga tcatgaagaa ttcgttcggg gtgtgaatga gggccgattg
     2101 catgtcacaa tgccaaccgc tgccgcaggc cagattgttt tggcgaaacc cgaaattttc
     2161 ggaacaggct gggttttcat gcgcggcttt ggatgtttgc ctctgatggc tgccgttggc
     2221 tttggccttt ggctcaagtg ggaatggtgg atatgggtta ttgttgtttt ggtggcatgg
     2281 gtgctgggtg gcagcttgaa gcgcgctttt tgcaaaacgg cagtagatga cgtggtgagg
     2341 cacgcgatga aagacccggc cttttatgac cagcttttgc tgaatcgtat tttgcgagtc
     2401 gaggcaaaaa tggaagccgg gcaatgaatc cggctcctcc tccgggtgaa tctccaaagg
     2461 gcagaagccg ggggagtgga atggagcgat ctggcaagag ctggcgcgaa gtgtattcaa
     2521 aaaatcactc cgccaaaaac gaacagtgaa acgggcgctg gcgggcgaaa aacgggcttg
     2581 cgttgtgtat tgcgatatac tatagtttat ttgataacac tcttgttgag actgaaaccg
     2641 ccatgaaaac cgcatcctct cacgttcccg tcgccatcct cgtccgcgtg tccacctcca
     2701 agcaggagac ggcgcggcag gtgtcagagt tgaaagccct ggctgattcc aaaggctggc
     2761 aggtggcggc aatctgtcag gaaagcggcg tctctggccg tgcggcagca gaagaccgcc
     2821 acggtctgca agaggcgctg gcactggcaa aggcgggcaa gatcaagaag gttctggtgc
     2881 atgaggtttc acgcctgggc cgccgtcctt ccgtggtgat gtcctttgtt gaggcgctgg
     2941 aagcctgctg tgtctccctc tactggcacg cgcaacgcat cgaaaccctg ctgccgaact
     3001 gcaagcgcaa cccggcggcg ggaatcatgc tggcgctgct tgcggagatg gcgcgggcgg
     3061 aagtggagac gatgcgggag cgcatcaata gcgggctggc tgaggcgagg cggaagggcg
     3121 tcacgctggg ccgccctgtt ggcagcggtc tggatgcccc ggcactactg gcaaagcaca
     3181 aggacgtggt gaaggcgctg aaagccgggc attctgtccg caacgctgcc aagatcacag
     3241 gcaaggggcc gtccacggtg caacgggtga aagcggcgct ggaagcatag ccgggcactg
     3301 aatccggctc ctgctccggg tgaatctgta aaggggagga gccggaggag gaggaaaggc
     3361 ggcggcattg gagggcgatt ccgggcgcgg cggggaagat ggaggaggaa cgatcataga
     3421 gccggaggcc agcggaaccg gaaagaactg gcaaggttca cgccgcgttc ttcgaagcgc
     3481 tgtggtatca gaaaaaggcc gggaactgga cggcagataa caaggtggca acggtcgggc
     3541 gcatacgaca gttcaaccca aagaaacagc cgcgccatgt ggagcagtgc cgggaaaaga
     3601 tagtgctcaa caaaccgggg gggggagggg gcggctggtg gcgaagcctg gactcgctac
     3661 aatggattca gggccaggaa ttttttcggc attggccggg ctgacattgc cagataggtg
     3721 acagttaagg gctgaaaact gtcaatttgt ggcattatct gtcactctct cttgagacaa
     3781 cccggcgcgc caaatcgcca tatatatctg attttcaatg atttggtgcg catctttcta
     3841 ttcgtgagca aagcaacaag gggggatttc taatccgctg gtcgttggtt cgatcccaac
     3901 cgggcgtgct ctttttttgt ccagcatggg tgacgcgagg gcgggtcggc gagcaaagca
     3961 gggtcattcc agggaaaaag cgcggattcg ccggaagcgg gagcggttgt gatggagaag
     4021 cagcactccg gccaggacga ggccgctgga cagccatgcc agcattttgc cggacgcggt
     4081 tcgcggtatc tggatgacgg ggctgctttc gccttcaacg agcagggtcc attgggcttg
     4141 tttgcctgtg ctggcgccgg cgcgagtcca gagcagcacg tcgtgaagac tgccccttgg
     4201 aactgcgaag acgagatccg gcaaatcagg cctccattcc cagacggctg tggtgagctg
     4261 gtgcggctgg ccgggggtgg ctggcgcgga aagcgcgggc gccccggcat cccattcgct
     4321 gcggcgctcc agtgtcatgg agcccagacc ggacagcgga accaagcccg ccggtgtgcg
     4381 caactggaga ctggtgcgca cggcctcctc ggcggctgat tcatcctgaa tcagcggatt
     4441 gcccgccacg tcggcggtga tctccagccg caccctgccg ctttccacat ccaagcgcgc
     4501 catcaggaac tcgcatccat gcccgcaagc gaacaggggg gccagaacgg tcccccctgc
     4561 cgcggataaa atgaagttcc gaaggcggcg catcatcctt tgcggttcca gcgcagcacg
     4621 cggccgaaga ggttccattc ggtttccaag atgttgccct gattgtcgaa ggtgatgccg
     4681 tgcggggagg tgacggtgcc ctggagccag tccttgggtt cgaccttggg tgtgtttgtc
     4741 tggccgggcg tgtcattggt gccaagctgg gccaccagta ttccctcctt gtcccagacg
     4801 ctcactcggc cggcgatttc ggccacgcac atgaggtcgc catgaaagga cgcgctgctg
     4861 gggcggcgca gcccctcgct ggcgatgaca ctgatgagat tgccgtcaag gtcgaggtgg
     4921 agcaggcggt tgttgccgcg gtcgcaggca agaacgcggg cgggttcaaa gcggcggtca
     4981 atgaagattt tgtgggtgtt cgccagcttg aggggctcga cagggccgcc gaagactttt
     5041 ttgaatttgc cggcgcggtc gaagacgaaa atccagctct ggccgtagcc gtccacgaca
     5101 tagatgtctc cgttcggggc cacatcgcag ttggtgaggg cgagcccttt cggaccagcc
     5161 ttgccttccg ggaatgcgct ggtgggaatt tccagcacga tggcgccgtc cagactggct
     5221 ttgatgactt tcttttcgcc cagcacggcg gcgaagatga actcccggcc ctcgtctttg
     5281 cggatgacga agccgtgcag ggagttcggc aggttcagcg ctctgacgaa cttgccgtcc
     5341 ggcccgtaaa cctgcacacc cgcgtccttg ttttccggac cctgcacgct cacataaaaa
     5401 ttcccggcgc tgtcacaggc gatctcgccg tggccattgc caatggtctc cctgcccggc
     5461 ggcggctgaa tgaagtcggg ctggaattca taagtcacct cgccggaggc ggtgcctagg
     5521 gcgagcagga aaaggagggt ggagcgcggg aacatggggc attcagaacg aagcgcggca
     5581 cgtccgtcaa tcacacccgc atggagaatt ccgcccgttg gcgcggtttg aaaacatgcg
     5641 tcaggctgtt cgcgaagtcc ggcacttgcc aagaggacgc ggcggcttat catcgtcggc
     5701 acggaagggt ggctgagtgg tctaaagtat ctgactcgaa atcagacgtg ggggcaactt
     5761 caccgtgggt tcgaatccca ccccttccgc cacaattttc cccgtcagga gtggagcgga
     5821 gtcgcggcgg catccctcat gccgcgcagg atgcggtggg tgcattgctc gaagtgctcg
     5881 aggatttcat cggcattcgt gcccggcggc agagcggcat ccgccagcac gggttgtccg
     5941 gcgagttgat cgagcagcac gagcacatct ttgagggaat gaaacaactg cgcctcctgc
     6001 tcggcctccg gcagcatgac aaaggcgaga ttgagcagcc agcggccgtc tcttttcagg
     6061 atgaagcgct gcattttgaa agtgctgctg ccgggctcat aaccctcctg acgcacgaga
     6121 tacggtccgt gcccgtttgg tccccacccc aggttcatca cctcgcagcg ggctgcgtca
     6181 ttgctcagcg cgggcatggc tcaggtgaaa aggatgtgcg tgccggctcc ggcggactcg
     6241 ttgtagaaat cgcccacggt cagcacgcca tcgacgcgtt ccatgaggtc ctccttcttc
     6301 agcttgaaca tgtccatcgc cagcttgcag gcataaattt tgccgccgga atcggagatg
     6361 agctccaaaa actcgcgcac cggcgggatg tcgagcttct ccatctcctt gttcatgaga
     6421 cgcgtggcaa aggccgaaac gccgggaatc gcacccatcc aggtgggaaa cggcatcgtc
     6481 atcggcaggc cgagcatggg catggccatg ttcagcgcgg aattgcccac ggtggacact
     6541 ttgaggctgt cgagcgtctt gttcgccagc gcattcaggc caaagaaggt gaagaacagc
     6601 gacgcctcga tgccctccat gcgggcgcca ttcgccatga tcagcgccgg gtaaatccca
     6661 tccagcgagc cgcggctcac gatgatggac atctttttca cgggttgttt gtcgtcggac
     6721 atagagttct tggttggtgg ttctttgttg atcaaatgca gccgacgggt ttcggaatgc
     6781 cggcgatctt cgaggacttc ttcagcgggc cgccggggaa gagatcatag agttccttcg
     6841 tcgtcacgac gccgctcttg cccatgctgc gaatcgtcag cgccgcgcct ttggcctgct
     6901 gctcacgcaa atagcgcacg accttcatgt gaccgtcgcc gagtgcaccg atgccttcct
     6961 ccaccgcgat ggcggcggcg atggcttcat tccactgcga catgtccgtc agatagcctt
     7021 cttcgttcac gtcgatctct gttcctgcga tgttcttttt catggtgggt tatgcgttga
     7081 gcggtttctt tttctggcgg ctgattccgg gcatgggcca ggctttgaga agcacattcc
     7141 aatacacatg acggaaggcg agcttgccga agtggttgag gcgggactcc tccagcagct
     7201 tcatggggcc gaaggcgaag gggaaattcc cctcgtgcgg ctcatactcg tagttgaagt
     7261 cgatgagcag cgccttgccg ttgccggatt cgatgaagca gttcgagtga ccgtcgaatt
     7321 cctccttcag cggctcgcct tggatgtggc gcatgacgtt gtcggcgagt gtttcgctct
     7381 caaagtgcgc caccgaaccc gccttcgacg cggggacgtt cgtggcatcg ccgatgacga
     7441 agacatctgg atgctgcagc gattgcagcg tgtgtttgtg cgtcgggatg aagtcgaggt
     7501 catcaccaag ggagctgcgg cgcacggcct cgtcgcccat gttcgtcggt gtggtcacca
     7561 gcaggtcgta gggcacctcg cgaccgtcaa agcacaccag cttgtcgttc tcctggtcca
     7621 cacgctcggc cacgaagtcg gtgacgagtt cgatgcgctt gtctgtgagc aggtgtccga
     7681 gctttttcga tgctttaggt ttcgtgaagg ctccagagag cggcgtcaca taggtaagcg
     7741 tcactttttc ccggatgcct ctttcgcgga aaaaggtgtc tgcaaggaaa gtgaactcca
     7801 gcggagccac cgggcacttg atcggcatct cattgatgtg caccacgacg cggccgccgg
     7861 ggaagctttg gagcttctcc cgcagggctt tggcaccatc aagggtgtag aagtcatgca
     7921 cgtttacccg ccacttcggc cccagcatgc cttccgtctc ctccggtgcc gtgcggcagc
     7981 ccgtggcgac gatgagcacg tcatacttca gctctcgacc atccttcagc gtcaccgcat
     8041 gatcctgcga cgtgatttga tccacctcag cctgcatgaa ctcgacgccg tgcggcatga
     8101 aatcccgcgt ctgccgcacg atctggcttt cgtcgtagat gccgaagggc aggaagagaa
     8161 agcccggctg gtagaggtgc ttttcggagc ggtccaccac cgtcatgcgc cactcggtgg
     8221 ccgaaagaag acgccgtaga tggttggcca tcatggtgcc tgcggtgccg ccgccgagga
     8281 tgaggaggtg cttgttcatg gttaggaaac gttgtcttgc aatgcgacta tatgagcgta
     8341 tgcgtatatg caagatttaa ctccaacccg attttgtcct tcatgagcgc ctctctcgaa
     8401 tccgacgccc gaggcatcat cctgccctgt cccgcctgcg gcaccgcgaa ccgcatcgcc
     8461 tacgccaaag tcagccagca aggccgctgc ggcacctgca aagccgatct gccgctcgtc
     8521 gctgcgccgg ccgagatcag cgacatcgag gccttcaccg cgctcgtcag ccagagcccg
     8581 ttgcccgtcg tgatcgactt ttgggcgccg tggtgcggcc cctgccagat gatggcaccc
     8641 gagtttgcca aagcggccgc acaagcggct ggcgaggctc ttttcgtcaa agtgaacacc
     8701 gacgagcagc cgcagatcgc cggacaattc cgcatccagg gcattccggc cttcgcattg
     8761 atccgcgatg gcaaactgac cgcccagacc agtgggttcc agcccgccgc tcgattgctg
     8821 gcgtggatgc ggcaggcgaa ctgatggaaa gggcggggct ggcgggagct tattgcttga
     8881 agttgccaca tctgattatg agccgcgcat tcccgttcac tgttctcatg cccttcatcc
     8941 tggcgggatt gattctggcg cagaccgcgc ccgcgccgaa gtccgtggcg ccgcatgtgc
     9001 cggcggtgtg gaaggcgggg ctggcctcgg tcattgtcac tccggaaaag ctgatctgga
     9061 tggccggtta cgcggcgcgc aaaaagccgg cggagggcaa gcagcaggag ttgttcgcca
     9121 aggcgctggc gctggaagac gaggcgggcg cgcgcatggt gtttgtgacg ctggatttga
     9181 tcggcgtgcc gcagagtctg cggcggcggc tggccgctgc tgtggaaaag gagcatggcc
     9241 tgcctccctc cgcgcttttg atgaacgcct cgcacacgca cagcggcccg caattgcgcg
     9301 gctcgccggt gacggacgcg gacttggaga atccgaaaat ccgcgatgcc tgggagtaca
     9361 cccaggacct cgaaggaaaa atcctcggca tcatccgcgc ggcactcgga gacatgaaac
     9421 ccgcgcgtct cacttggaac aaggcgcgct gcggctttgc catgaaccgc cgccgggact
     9481 acacgctgcc gccggagcat ccgaacgcga acaaggcgcc caaccccgcc ggggtggtgg
     9541 atcacgaggt tccctccctt cgcattgagt ctccagaggg tgcgccgcgc ggtgtgctgt
     9601 ttggctacgc ctgccacaac acctgcctgg gcttttacaa ctggtgcgga gactacgcgg
     9661 gctacgcgca ggaatatctg gagaagcacc gtccggactt tcaggcaatg tttctcacag
     9721 gctgcggcgg cgaccagaat ccttatccgc gccgctccga agtggtgcct ggggtcagcg
     9781 atgtggagct ggcgcagcag cacgggcgct cgctggcgaa cgcggtggag gtggccatat
     9841 ccgcgaatcc gatgcccgtc accggcaggc tgcgctccgc ttatgaggag gtcacgctgc
     9901 attatgccag ggatggcaag gagccgcacg cctaccccgt gcaggtggtg aagtttgggg
     9961 atgacctgac gctggtggct ctcggcagcg aggtggtggt ggactactcc ctgcggctga
    10021 aaaaagagct ggccggcaag gcgggtgtgt gggtggccgg atacagcaac gactacacag
    10081 gctacatccc cagcctgcgc atccagaaag agggcggtta cgaggccgcc gcaggctggg
    10141 cggaggatgt ggaggagcgc atcatcagca aggtgcatga gctgcacaag agcctgccat
    10201 gaatccactc acataccgcc gcgctggctg gagcgtgtca tgcactctag aagaggttgt
    10261 tgagcagtag gaagacagcg gctagccagt gcaggccggc gagggtgctg ggcaggcgtt
    10321 catagaactg actgttcgct ggctcgtcgg cctctttggt tcttggggca tccgcagctc
    10381 atgctcgcgc cttgttcgag tgtgcggcgc tttttcacct cgaagttcat cacacgatct
    10441 aattcacagc gcgcagacac caaggctgat ttccaccgat gacatggacc ctgcggcggt
    10501 cgccaaggac aagacgcggc gggatcaggc ctccggcatg atcacgttga cggaaacact
    10561 gcgcgagtat ctgcggaaca tcaatttttt gagcaatgcc gacctgttca actactgcaa
    10621 catgatccac gccaacgcgc gggagcgcgc ccggcgcggc gtccctggag ccgacaccat
    10681 ttttgccgac ctgcaggcgc actttcccgg ccgccgccgc gccagcaccc ccgccccgcc
    10741 g
//
LOCUS       DAOMYJ010000184        10724 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Prosthecobacter sp. isolate AMR_MDS_5629
            NODE_2048_length_10724_cov_20.267129, whole genome shotgun
            sequence.
ACCESSION   DAOMYJ010000184 DAOMYJ010000000
VERSION     DAOMYJ010000184.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867850
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prosthecobacter sp. (wastewater metagenome)
  ORGANISM  Prosthecobacter sp.
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Verrucomicrobiaceae; Prosthecobacter.
REFERENCE   1  (bases 1 to 10724)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10724)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.30
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:34:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,742
            CDSs (total)                      :: 4,684
            Genes (coding)                    :: 4,676
            CDSs (with protein)               :: 4,676
            Genes (RNA)                       :: 58
            tRNAs                             :: 55
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10724
                     /organism="Prosthecobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2048_length_10724_cov_20.267129"
                     /isolate="AMR_MDS_5629"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1965333"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..935
                     /locus_tag="PLP58_16530"
     CDS             <1..935
                     /locus_tag="PLP58_16530"
                     /inference="COORDINATES: protein motif:HMM:TIGR03696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="RHS repeat-associated core domain-containing
                     protein"
                     /protein_id="HRJ10185.1"
                     /translation="LETMTWLSRDPAGFVDGPNLYAYVQQNPWTAFDPLGLWGEAGHF
                     WTTYGVAVAAGLPPQKAFTLAYYSQVPDEHVALDAWSLWKSDGNTASMFRTTNRRETL
                     NYIGEGRDLHDWSMAVQRNIHSLHGGDAEAVRRRQGDLRQMVQNGGSSDMERGLLIHA
                     YGDSYAHVNASGSAYSTGNGHGWDSRSNATDPDLIGNRPQRHRDHMGELYSALNEGGG
                     KPELLDSLVETARGIQSRQGNDTNNNDFGTAIRGNELFGWNTLPQAMRDYNPNAPMSR
                     IPATLPDPTADQVEQTMNRQEEVYERNDPSRNDD"
     gene            901..1230
                     /locus_tag="PLP58_16535"
     CDS             901..1230
                     /locus_tag="PLP58_16535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ10186.1"
                     /translation="MSEMTHQETTTNPTRIIHVAQASGSLLAIYTLWRIWSSMDAGDL
                     VKMSAAEFLTPIVLINAATCLFCCGWVVGIVTSNKGFSGCSRLGWSQIGWFVFAASDY
                     VAPFYFY"
     gene            1662..2120
                     /locus_tag="PLP58_16540"
     CDS             1662..2120
                     /locus_tag="PLP58_16540"
                     /inference="COORDINATES: protein motif:HMM:NF025789.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUKH-3 domain-containing protein"
                     /protein_id="HRJ10187.1"
                     /translation="MRFSDRLLDRLKQAGWNEGRSSFQNLHVFQTALGDVLHSTAYRV
                     LLEFAGLDIGRRVFFEPYYATLALRSRQLLPPNFRETLCPVALTTYWSDTTVWIDENG
                     RVFLLEPDELSYLAASMDAALEILVLDGKPEPVPQELHPGHWDIENSDNL"
     gene            complement(2523..3224)
                     /locus_tag="PLP58_16545"
     CDS             complement(2523..3224)
                     /locus_tag="PLP58_16545"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HRJ10188.1"
                     /translation="MRTLAIGDIHGCSTALRTLLEAVQPGADDVLVTLGDYVDRGPDT
                     KGVLDTLIGLEKTTQLIPLTGNHEILFMEAMFERLNLGAWEAIGGLQTLQSYCGDKPV
                     SWNLIPEAHVEFIAGRCRRLHETDTHIFVHANLNAMLPLHEQSDDWLFWTRFSDSHPH
                     FSGRTMICGHTAQHSGLPAVRPGGICLDTWVYNPAGWLTCMDVASGQITQASQRGELR
                     TLSLDALEMATLRMD"
     gene            3327..5084
                     /gene="ptsP"
                     /locus_tag="PLP58_16550"
     CDS             3327..5084
                     /gene="ptsP"
                     /locus_tag="PLP58_16550"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR01417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008965 - phosphoenolpyruvate-protein
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0006808 - regulation of nitrogen
                     utilization [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HRJ10189.1"
                     /translation="MSQAATPDIRQWQGTPVSAGVAHAPVHVLKDHFDEPDAEPIQPG
                     DVERELERWHAALDLTHQEIEALQARVREEQGNDEADIFETHLLILQDNSVRKQVEKT
                     VREKLVCVDAVYYRLMRRHMDALRGLSDSYLRERFVDIKDITQRVMRHLRGELLDHPM
                     FEDPVIIVARDLTPSDTVQLDRSKVLGFAVETGSANSHAAIIARSLGLPAVVRLHNIC
                     EELHSGDVVLLDGEEGLLILNPDAATLAGYRSREEEAEQFEDALHIERHAAAVTTDGQ
                     PLEICANAEFVEEMKAIRDCGAAHVGLFRTEFLHLEDPEGSEDWLAEAYTAAVRAMHP
                     GQVIFRTLDIGGDKLDAEMLAEAEPNPFLGWRGIRVSLGRPALFKRQLRAMLRAAAHG
                     EVGIMFPMVSGVEEVRQARALLEECAAELRARGIEPPGVEIGAMIEIPSAALSADLIA
                     AEVDFLSLGTNDLVQYTLAVDRLNERVAGLYQPTHPAILRLIAVTVEAARQAGIRACI
                     CGEMAADVEMTPLLIGLGLNELSVASGQVARVKHAVRRLHSGECRELAKTARRLASAP
                     EVLELTRRKARQCYPELFE"
     gene            5137..5526
                     /locus_tag="PLP58_16555"
     CDS             5137..5526
                     /locus_tag="PLP58_16555"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: protein motif:HMM:NF004064.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004126 - cytidine deaminase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0009972 - cytidine deamination [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HRJ10190.1"
                     /translation="MPADLLTLAREAAAKAHAPYSRFQVGCALETEAGGMFQGCNVEN
                     ASYGLTICAERAAIFQAVAAEGGGMRVRRLAVVCPGHEFPPCGACRQVIAEFSLPGGA
                     TEVTFLQNGRAVTRTAAELLPEAFGLG"
     gene            complement(5535..6983)
                     /locus_tag="PLP58_16560"
     CDS             complement(5535..6983)
                     /locus_tag="PLP58_16560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009958064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1501 domain-containing protein"
                     /protein_id="HRJ10191.1"
                     /translation="MNTVPHHELLTDRREFLRRGGAGFGALALAHLLGESPLAAALGD
                     AGGKPGSPMSAKLAHRAGRAKSVIFLFMEGGPSHLDTFDPKPLLRKLAGQQLPESFGS
                     VITAMGESRAPLLADKREWKQHGRSGLWISDWCPHVARMADELCVIRSCVSDGINHAG
                     GVCQMNTGSIFGGRPSLGAWVNYGLGTLNQNLPAFVVLKDSDAQVVNGVRNWGSGFMP
                     AVYQGVSFDAEGSPISNLMTPKSISEQRQLSKLAFLGRLNRDHGRSRASNSDLDARIK
                     SYELAFRMQAEAPEAVDLGRESEATKKLYGLDQPQTAVFGRNCLLARRLVERGVRFVQ
                     LYSGAGSKWDAHDKIEENHTRLFGQTDKPVAGLLMDLKARGLLEETLVIWGGEFGRTP
                     MSEKGDGRDHNPTGFTMWMAGGGIQGGQLIGATDDLGLRAVDQKMHVHDFHATILHLM
                     GLDHTRLVYKYKGRPERIDLNEGHAYTKMRMA"
     gene            complement(7086..8123)
                     /locus_tag="PLP58_16565"
     CDS             complement(7086..8123)
                     /locus_tag="PLP58_16565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009965130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ10192.1"
                     /translation="MPASPKKSAPAKAAPAPGFKQPPVLFDKTQAMIQKIEKRLGGKL
                     VTYWNGSRGSVCHNDVTALFRLLEHIGPQDTLYLFVKSDGGNGRAALRMVNLIRRYCK
                     KLVALAPLECASAATMIALGAEEIQMGPIAYLTAVDTSLTHDLSPIDRDNDRVSVSLD
                     EVKRVISLWRHNEGAGSDMNPYHALFQYIHPLVIGAVDRADSLSLMLCEEILSHHVRD
                     AKKRRQIATTLNSKYPSHAYPVLIEEARRIGLNAVPMDPGTASMLLDLNHIYSEMGQK
                     ATTDISDRESHGNEIINILEREHCQIYYQQDKDWFYRAEERRWIVLNDDSSWHQITGS
                     KGRMDHEVLFM"
     gene            complement(8217..9404)
                     /gene="sucC"
                     /locus_tag="PLP58_16570"
     CDS             complement(8217..9404)
                     /gene="sucC"
                     /locus_tag="PLP58_16570"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009964327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-forming succinate--CoA ligase subunit beta"
                     /protein_id="HRJ10193.1"
                     /translation="MNIHEYQAKELFQKFGVATPAGKVAATADEAGRAAEELGGAGLV
                     VKAQVHAGGRGKGAFKNGFKGGVHVVNTAAEARDIAEKMLGQTLVTHQTGPEGKLVSK
                     VLVAKSVDIVKEHYIAILFDRGSSSHALIASTEGGMNIEEVAEKTPEKIIKEFISPAF
                     GLQAYQARKVAARLGLTGKLAAQAAKLFLSLWSLYLKSDCSLVEVNPLAITADGQVVA
                     LDAKFNFDDNALYRQKEVTAMRDTTEEDPREVAASEFGLNYIGLDGDIACLVNGAGLA
                     MSTMDIIKLHGGEPANFLDVGGGATKEQVAAAFKIILGDPNVKGILVNIFGGIMDCDI
                     IAHGIIAAAREVSLTIPLVVRLEGNNVEAGKATLAASGLAITSADGLTDAAKKIVAAV
                     KAA"
     gene            9652..10254
                     /locus_tag="PLP58_16575"
     CDS             9652..10254
                     /locus_tag="PLP58_16575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007417133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HRJ10194.1"
                     /translation="MGRTSDAKERLIEAVTELIWTGSYGSTSVDQICERAGVKKGSFY
                     HFFESKTELAVTGIEHGWTEHHKKLDQAFSPVIPPLERIWRCFREFRQEQEEMKEKHG
                     RVLGCPIHSLGAEVSTTDDKLRDKLQEILAQFIRYYESAIRDAHYQGVIEAPDAPALA
                     RIVFAYGEGLLLHARIWNDLSFLDEMEAGARRILGVKGED"
     gene            10326..>10724
                     /locus_tag="PLP58_16580"
     CDS             10326..>10724
                     /locus_tag="PLP58_16580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007417135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin/lipoyl-binding protein"
                     /protein_id="HRJ10195.1"
                     /translation="MKTQAFLPLILLLAACGRPSAGPPMGEMPPAQVTLGVVEEKKLV
                     EWEEFTGRVEPVETVELRPRVSGYITGVHFEAGSLVRKEEVLFTIDPRPFQTRLDGAR
                     AELARTEAAAKAARLEFDRVSSLLAAKAIAP"
BASE COUNT         1874 a         3208 c         3601 g         2041 t
ORIGIN      
        1 atctggaaac gatgacctgg ctgagccgcg acccggcggg ctttgtggac ggcccaaatc
       61 tctatgccta cgtgcagcag aacccgtgga cggcttttga tcccctgggg ttatgggggg
      121 aggctggaca tttttggact acttacggcg ttgctgttgc ggccgggctg ccacctcaga
      181 aggcgtttac attggcctat tattcacaag ttcccgatga acacgttgcg ctcgatgctt
      241 ggtccctgtg gaaatcggat ggtaatacgg caagcatgtt tagaactacc aaccgccggg
      301 agactctgaa ctacatcggg gagggacgcg atcttcatga ttggagcatg gcggttcaac
      361 gaaatattca ctcgctgcat ggaggtgacg ctgaggcagt gaggcgaaga cagggcgacc
      421 tcaggcagat ggtgcaaaac ggtggttctt ccgacatgga acgtggtttg ctcattcacg
      481 cttatggtga ctcctatgca catgtgaacg cgtcaggcag tgcctattca acaggcaatg
      541 gtcacggttg ggattcgaga tcaaacgcta ccgatccaga cttgattggg aatcgccccc
      601 aaagacatcg tgatcacatg ggcgagctat actccgcctt aaatgaagga ggaggaaaac
      661 ctgaactgct ggactcgcta gttgagaccg ctcgaggtat tcaatccaga caaggaaacg
      721 acacgaataa taatgacttt ggcactgcga taaggggtaa tgaattgttc ggatggaaca
      781 cacttccgca agcaatgcgt gactacaatc caaatgcgcc catgtcacga ataccagcaa
      841 cactgcccga tccaaccgca gatcaagtgg agcagaccat gaatagacaa gaagaagtgt
      901 atgagcgaaa tgacccatca agaaacgacg actaatccaa caaggatcat ccatgttgcc
      961 caggcatcag gatcactttt ggcaatctac acactatggc gaatttggtc gtcgatggac
     1021 gctggagacc ttgtgaaaat gagcgctgct gaatttttga cgccgattgt tttgatcaat
     1081 gcggcaactt gcttgttctg ttgcggctgg gtcgttggaa tagtgacttc caataaaggc
     1141 ttctctggat gctctcgtct ggggtggtcg caaatcggat ggtttgtttt tgctgccagt
     1201 gattatgtgg ccccctttta cttttactga gaagttccat tccccagctc gtcgcgcagc
     1261 tcctcggctc cgcaccgttg ccagccgtga tgatgctccg cccgctcccg cccccgccgc
     1321 aagggagtgt gctgctcatc gcagcagctc cgcacccgga tgaaccgccc ttccaggaat
     1381 agtagccagg caaaaagtag ccatggtgcg cgagggcgga actgcgcgag ctggacagac
     1441 gggaaagtga tgagaagctg gccaccatta aaccgacgcc acagccgaat gaagatcatt
     1501 aaatttgtca agcagttgta cactgacccc gtttccctga gcggaggcag ttcggctgcg
     1561 gccttggttg gtggcccgaa agctggccaa ataattccgg catgcaaccg ttgcgctcca
     1621 gtcctacaaa aaagaaacat tcgagaagta gttgattagc tatgagattt tcagacaggc
     1681 ttttggaccg cctgaagcag gcaggttgga acgaagggcg atcttctttc caaaatttgc
     1741 atgttttcca gacagctctg ggtgatgtgt tacatagcac tgcttaccgc gttctactcg
     1801 aatttgcagg cttggatatt ggtaggagag ttttttttga gccgtattac gccactttgg
     1861 cactaaggtc acgtcagttg ctgccaccaa attttcgtga aactctatgc ccagttgcac
     1921 tcacaacgta ttggtctgac acgacagttt ggattgatga aaacgggcgt gtcttcctgc
     1981 tggagccaga tgagctaagt taccttgctg cttcaatgga tgcggcattg gaaatactgg
     2041 tgcttgatgg taaaccagaa ccagttccgc aagagcttca tccaggtcat tgggacattg
     2101 aaaacagtga caatctatag cataaaaaat ggacgctccg ctatgctgtg gaatagttgc
     2161 tggaatagtt gccaaaaaaa gtagccaggc gttttgtaat gcaaatccgt gcaatccatt
     2221 tgttttcaaa gagttgaaag cctggcttac tgctcacatc tggcgcgcgg tcctcgcttc
     2281 agcctccgtt ctcaaacccc ttgccccaga tccgcaaggg cccatgatgg ccgccttgcc
     2341 cgcgaatccc tccgttttcg atcttttccg ccgcccctcc ctgtggcgtg atgcgctggc
     2401 ggcgctgcag gccgccgtcg ttttgctcat gcccagtcac gcgcgcggcg ggccggaggg
     2461 ggtgactttg ccgggaggcg cggcattttc ccccgcctgc gatgctgacg ggcgccattg
     2521 cctcagtcca tcctcagggt cgccatttcc agcgcgtcca ggctgagagt gcgcagctcg
     2581 ccgcgctggc tggcctgggt gatctggccg gaggccacgt ccatgcaggt gagccagccg
     2641 gcggggttgt agacccaggt gtccaggcag atgccgccgg ggcgcacggc gggcaggccg
     2701 gagtgctgcg cggtgtggcc gcagatcatg gtcctgccgg agaagtgcgg gtgggagtcg
     2761 gagaagcgcg tccagaagag ccagtcatcg ctctgctcat ggaggggcag catggcgttg
     2821 aggttggcgt ggacgaagat gtgggtgtcc gtctcatgca ggcggcggca ccgcccggcg
     2881 atgaactcga cgtgcgcttc ggggatgagg ttccaggaga ccggcttgtc accgcagtag
     2941 gattgcagcg tttgcaggcc gccgatggcc tcccaggctc ccaggttgag cctttcaaac
     3001 atggcctcca tgaacaggat ctcgtggttg ccggtcaggg gaatgagttg ggtggtcttt
     3061 tccaggccga tgagggtgtc cagcacgccc ttggtgtcgg ggccgcggtc cacataatcc
     3121 ccgagcgtca ccagcacgtc gtccgcgccc ggctgcacgg cttccagcag ggtgcgcagg
     3181 gcggtggagc agccgtgaat gtcaccgatg gccagtgtcc gcatggcgca ctttcgcgcg
     3241 cgcggcgtcc cgcgcaagcc ggagcatggg ccgggcgctg tttcttcatt tgcgccgccg
     3301 ccgtgtccgg ctgaaaatgc cgccgcatga gccaggccgc cactcccgac atccgccaat
     3361 ggcagggcac tccggtttcc gccggagtgg cgcacgcgcc ggtgcatgtg ctcaaggacc
     3421 acttcgacga gccggacgcc gagccgatcc agcccgggga tgtggaaagg gagctggagc
     3481 gctggcacgc ggcgcttgat ctgacgcacc aggagatcga ggccctgcaa gcccgcgtgc
     3541 gcgaggagca gggcaatgac gaggccgaca tcttcgagac gcacctgctc atcctccagg
     3601 acaactccgt gcgcaagcag gtggagaaga ccgtgcgcga gaagctcgtg tgcgtggacg
     3661 cggtgtatta ccgcctcatg cgcaggcaca tggacgccct gcgcgggctg tccgactcct
     3721 acctgcggga gcgcttcgtg gacatcaagg acatcaccca gcgtgtgatg cgccacctgc
     3781 gcggcgagct gctggaccac cccatgtttg aggatcccgt catcattgtg gcgcgcgacc
     3841 tcacgccctc ggacaccgtg cagctcgacc gcagcaaggt gctcggcttc gccgtggaga
     3901 ccggcagcgc caactcccac gcggccatca tcgcccgctc cctcggcctg cccgccgtcg
     3961 tccgcctgca caacatctgc gaggagctgc actccgggga tgtggtcctg ctcgacggcg
     4021 aggagggcct gctcatcctc aatcccgacg ccgccaccct ggccggctac cgcagccgcg
     4081 aggaggaggc cgagcagttc gaggacgccc tgcacattga gcgccacgcc gccgccgtca
     4141 ccaccgacgg ccagccgctg gaaatctgcg ccaacgccga gttcgtcgag gagatgaagg
     4201 ccatccggga ctgcggcgcc gcccatgtcg gactcttccg caccgagttc ctccacctgg
     4261 aggaccccga aggctccgag gactggctgg ccgaggccta cacggccgcc gtgcgcgcca
     4321 tgcatcccgg ccaggtcatc ttccgcacgc tcgacatcgg cggcgacaag ctggacgccg
     4381 agatgctcgc cgaggccgaa cccaacccct tcctgggctg gcgcggcatc cgcgtctccc
     4441 tgggccgccc cgccctcttc aagcgccagc tccgcgcgat gctgcgcgcc gccgcgcatg
     4501 gcgaggtggg catcatgttt cccatggtca gcggcgtgga ggaggtgcgc caggccaggg
     4561 cgctgctgga ggagtgcgcc gccgagctgc gcgcgcgcgg catcgagccg ccgggcgtgg
     4621 agatcggcgc catgatcgag atccccagcg ccgcgctctc cgccgacctc atcgccgccg
     4681 aggtggactt cctcagcctg ggcaccaatg acctggtcca gtacaccctc gcggtggacc
     4741 ggctcaacga gcgcgtcgcg ggcctctacc agcccaccca cccggccatc ctgcgcctta
     4801 tcgccgtcac tgtcgaggcg gcgcgccagg ccggcatccg cgcctgcatc tgcggggaga
     4861 tggccgccga tgtcgaaatg accccgctgc tcatcggcct cggcctcaac gagctcagcg
     4921 tcgcctcagg ccaggtggcg cgcgtgaaac acgcggtgcg caggctgcac tccggcgaat
     4981 gccgcgagct ggccaaaacc gcccgccgcc tggcctccgc tcccgaggtg ctggagctca
     5041 cccgccgcaa ggcgcggcaa tgctaccccg agctcttcga gtgagccttc cctgcctgag
     5101 cgcaacatcc accctccctc ccgcgccctc accgtcatgc ccgccgatct gctcaccctg
     5161 gcccgggaag ccgccgcgaa ggcgcacgcg ccgtattcgc gcttccaggt ggggtgcgcg
     5221 ctggagacgg aggcgggcgg aatgttccag ggctgcaatg tggagaacgc cagttacggg
     5281 ctgacgatct gcgccgagcg cgcggccatt ttccaggcgg tggccgcgga aggcggcggg
     5341 atgcgcgtcc gcaggctggc ggtggtctgc cccggccatg agttcccgcc ctgcggcgcg
     5401 tgccggcagg tcatcgcgga gttctccctg cccggcggcg ccacggaggt gacctttctg
     5461 caaaacgggc gcgctgtgac gcggactgcc gcggagctgc tgccggaggc gttcggcctt
     5521 ggctgagccg ggcctcaggc catccgcatc tttgtgtagg cgtggccttc gttgaggtct
     5581 atgcgctcgg ggcggccttt gtatttatag acgagccggg tgtggtccag gcccatgagg
     5641 tgcaggatgg tggcgtggaa gtcatgcaca tgcatcttct ggtccacggc gcgcaggccg
     5701 aggtcgtcgg tggcgccgat gagctggccg ccctggatgc cgccgcccgc catccacatg
     5761 gtgaagccgg tggggttgtg gtcgcgcccg tcgcccttct cgctcatggg ggtgcggccg
     5821 aactcgccgc cccagatgac gagcgtctcc tccagcaggc cgcgcgcttt gaggtccatg
     5881 agcaggccgg cgacgggctt gtcggtctgg ccgaagaggc gggtgtggtt ttcctcgatc
     5941 ttgtcgtgcg cgtcccactt gctgccggcg ccggagtaga gctgcacaaa gcgcacgccg
     6001 cgctccacga ggcggcgggc cagcaggcag ttcctgccaa agacggcggt ctgcggctgg
     6061 tccaggccgt agagtttttt ggtggcctcg ctctcccggc cgaggtccac ggcctcgggc
     6121 gcctccgcct gcatgcggaa ggccagctcg taggacttga tgcgggcgtc gagatcggag
     6181 ttcgaggcgc ggctccggcc gtggtcgcgg ttgagcctgc cgagaaaggc cagcttggag
     6241 agctggcgct gctcgctgat gcttttcggc gtcatcaggt ttgaaatggg ggagccttcc
     6301 gcgtcgaagc tcactccctg gtaaacggcg ggcatgaagc cgctgcccca gttgcgcacg
     6361 ccgttgacca cctgcgcgtc gctgtccttg agcacgacaa aggcgggcag gttttgattg
     6421 agggtgccca ggccgtagtt cacccacgcg cccagggacg ggcggccgcc gaagatgctg
     6481 ccggtgttca tctggcacac gccgcccgcg tggttgatgc cgtcggagac gcaggagcgg
     6541 atgacgcaca gctcgtcggc catcctcgcc acatgcgggc accagtccga gatccacagg
     6601 ccgctccgcc cgtgctgctt ccactcccgc ttgtcggcca gcaggggcgc acggctctcg
     6661 cccatggcgg tgatgacgct gccgaagctc tccggcagtt gctgcccggc cagcttccgc
     6721 agcagcggct tgggatcgaa cgtgtcgagg tggctggggc cgccctccat gaacagaaag
     6781 atcacgcttt tggcgcggcc cgcgcggtgc gccagcttcg cgctcatggg gctgcccggc
     6841 ttgccgcccg cgtcgccaag cgcggctgcg agaggactct cccccagcag atgggcaagg
     6901 gccagcgcgc cgaaaccggc gccgcccctg cgcaggaatt cgcggcggtc cgtgagcagt
     6961 tcgtggtggg ggacggtgtt catgacgggg gagtttacac acgatcaagg ctgccgcgcg
     7021 ccggttttgg acaggaaatg tgaagcccgg cgcccgaaac agggttctgg cgggcggggg
     7081 gggaatcaca tgaagaggac ttcgtggtcc atgcggcctt tgctgccggt gatctggtgc
     7141 caggagctgt catcgttgag gacgatccag cggcgctcct cggcgcggta aaaccagtcc
     7201 ttgtcctgct ggtagtagat ctggcagtgc tcgcgctcga ggatgttgat gatctcgttg
     7261 ccgtgggact cgcggtcgct gatgtcggtg gtggccttct gccccatctc gctgtagatg
     7321 tggttgaggt cgaggagcat ggaggcggtg ccggggtcca tgggcacggc gttgaggccg
     7381 atgcggcgcg cctcctcgat gagcacgggg taggcgtggg aggggtattt ggagttgagg
     7441 gtggtggcga tctggcggcg ctttttggcg tccctgacat ggtgggagag gatctcctcg
     7501 caaagcatga gggagaggga gtcggcgcgg tccacggcgc cgatgacgag ggggtggatg
     7561 tactggaaga gcgcgtggta ggggttcatg tcactgcctg cgccctcgtt gtgacgccag
     7621 aggctgatga cacgcttgac ctcgtcgagg ctgacgctga cgcggtcgtt gtcgcggtca
     7681 atgggggaga ggtcgtgggt gagggaagtg tccacggcgg tgaggtaggc gatgggcccc
     7741 atctgaatct cctcggcgcc gagggcgatc atggtggcgg cggaggcgca ttcgaggggg
     7801 gcgagggcga cgagcttttt gcagtagcgg cggatgaggt tcaccatgcg cagggcggcg
     7861 cggccgttgc cgccgtcgga cttgacgaaa aggtagaggg tgtcctgagg gccgatgtgc
     7921 tcgagcaggc ggaagagggc ggtgacgtcg ttgtggcaga cggagccgcg cgagccgttc
     7981 cagtaggtga cgagtttgcc gccgaggcgc ttttcgatct tttggatcat ggcctgtgtt
     8041 ttgtcgaaga gcacgggggg ctgcttgaac ccgggcgcgg gcgcggcttt ggcgggggcg
     8101 gattttttgg gggaggctgg catgagggtt ggatgtgggg aaagccgcgc gcggatgcgc
     8161 ggcagcgcgc gggcacgctg ttcgaacggg ctcgatggag gcccgccccg gcggggtcag
     8221 gcggctttga cggcggcgac gatttttttc gcggcgtcgg tgaggccgtc ggcgctggtg
     8281 atggcgaggc cgctggcggc gagggtggct ttgccggctt cgacgttgtt gccctcgagg
     8341 cggacgacga gggggatggt caatgacacc tcgcgcgcgg cggcgatgat gccgtgggcg
     8401 atgatgtcgc agtccatgat gcctccgaag atgttgacga ggatgccctt cacattcggg
     8461 tcgccgagga tgattttgaa ggcggcggcg acctgctcct tggtggcgcc gccgccgacg
     8521 tcgaggaagt tcgcgggctc gccgccgtgg agcttgatga tgtccatggt gctcatggca
     8581 aggccggcgc cgttgacgag gcaggcgatg tcgccgtcga ggccgatata gttgaggccg
     8641 aactcgctgg cggcgacttc gcgcgggtcc tcctcggtgg tgtcgcgcat ggcggtgacc
     8701 tccttctggc ggtagagggc gttgtcgtcg aagttgaact tggcgtcgag ggccacgacc
     8761 tggccgtcgg cggtgatggc gaggggattg acctcgacga gggagcagtc gctcttcaaa
     8821 taaaggctcc agagggaaag gaagagcttg gcggcctggg cggcgagctt gccggtcagg
     8881 ccgagccggg cggcgacttt gcgcgcctgg taggcctgga gtccgaaggc ggggctgatg
     8941 aactccttga tgatcttttc cggcgtcttt tcggcgacct cctcgatgtt catgccgccc
     9001 tcggtgctgg cgatgagggc gtggctgctg ctgccgcggt cgaagaggat ggcgatgtag
     9061 tgctccttga cgatgtccac ggatttggcg acgaggactt tgctgacgag cttgccctcg
     9121 ggcccggtct ggtgggtgac gagagtctgg ccgagcattt tttccgcgat gtcccgcgcc
     9181 tcggcggcgg tgttgacgac atggacaccg cctttgaagc cgtttttgaa ggcgcccttg
     9241 ccgcgcccgc cggcgtgaac ctgggctttg accacgaggc cggcgccccc aagctcctca
     9301 gcggccctgc ccgcctcgtc agcggtggcg gcgactttgc ccgcgggtgt ggcgacgccg
     9361 aatttctgaa agagttcctt ggcctggtat tcgtggatgt tcatggggga aagcggcgcg
     9421 cagaatagaa agccgctccc cacggtcaag cctgcgcgcg gattctttgc ccagccggga
     9481 aacatccaga cggcggcggt gaggatgagc ttgaaggaaa ttccgcggaa tgccgcgccc
     9541 ggtgaaaccg cgcagggtcg gtgccggcgg acagcacccg ttttcaatcc cgaagctgtg
     9601 cctcaaaaag aaaatagttg accaatcgtc tagttaccgc tttagagcgt catgggacga
     9661 acatccgacg ccaaagaacg cctcatcgaa gctgtgaccg agctgatctg gaccggcagc
     9721 tacggcagca cctccgtgga tcagatatgt gagcgggctg gagtgaaaaa aggcagcttt
     9781 taccacttct tcgaatcgaa gaccgagctc gctgtgaccg gcatcgagca tggctggacg
     9841 gagcatcaca agaagctgga ccaggcattc tctcccgtga tcccgcctct ggagcgcatc
     9901 tggagatgct tccgcgaatt ccgccaggag caggaggaaa tgaaggaaaa gcatggtcgc
     9961 gtgcttggct gccccattca ctccctgggc gccgaggtga gcaccacgga tgacaaactg
    10021 cgggacaagc tgcaggagat cctggcccag ttcatccgct actatgaaag cgccatccgc
    10081 gacgcccact accagggcgt cattgaggcg cccgacgctc ccgctctggc acggatcgtg
    10141 tttgcctacg gcgagggcct gctgctgcac gcccgcatct ggaacgattt gagctttctc
    10201 gacgagatgg aggccggcgc ccgccgcatc ctcggcgtca aaggcgaaga ctgagtgttc
    10261 aacgcctcgt tttaaccaac tggtcaactt tacttccggc aaatccccaa aaaacaaagc
    10321 acctcgtgaa aactcaagcc ttcctcccgc tcatcctgct cctcgccgcc tgcggcaggc
    10381 cgtccgccgg cccgccgatg ggcgaaatgc cgcccgcgca agtcaccctt ggcgtggtcg
    10441 aggagaagaa actcgtcgaa tgggaggagt tcaccggacg cgtcgaaccg gtggagaccg
    10501 tggaactgcg cccgcgtgtg tccggttaca tcaccggggt gcattttgag gcgggctccc
    10561 tcgtgaggaa agaggaggtg cttttcacca ttgatccgcg ccctttccag acgcggctcg
    10621 atggcgcgcg cgccgagctg gcgcggaccg aggcggcggc aaaagcggcc aggctggagt
    10681 ttgaccgcgt gtcgtccctg ctggccgcca aggccatcgc gcct
//
LOCUS       DAOMYJ010000185        10589 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Prosthecobacter sp. isolate AMR_MDS_5629
            NODE_2082_length_10589_cov_19.997057, whole genome shotgun
            sequence.
ACCESSION   DAOMYJ010000185 DAOMYJ010000000
VERSION     DAOMYJ010000185.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867850
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prosthecobacter sp. (wastewater metagenome)
  ORGANISM  Prosthecobacter sp.
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Verrucomicrobiaceae; Prosthecobacter.
REFERENCE   1  (bases 1 to 10589)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10589)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.30
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:34:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,742
            CDSs (total)                      :: 4,684
            Genes (coding)                    :: 4,676
            CDSs (with protein)               :: 4,676
            Genes (RNA)                       :: 58
            tRNAs                             :: 55
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10589
                     /organism="Prosthecobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2082_length_10589_cov_19.997057"
                     /isolate="AMR_MDS_5629"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1965333"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(156..1466)
                     /gene="xylA"
                     /locus_tag="PLP58_16585"
     CDS             complement(156..1466)
                     /gene="xylA"
                     /locus_tag="PLP58_16585"
                     /EC_number="5.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009959507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0009045 - xylose isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA];
                     GO_process: GO:0042732 - D-xylose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xylose isomerase"
                     /protein_id="HRJ10196.1"
                     /translation="MNEAFPDIPRIQYEGPKSKNPLSFKHYNADEVVAGKTMREHLRF
                     GIAYWHAMRNSLADPFGVGTAQRPWDDGTSSIANAQRRVQACFEFMDKLGVDYYCFHD
                     RDVAPEGETLAESNANLDAVADELEKAQKATGKKLLWGTACLFAHPRYNQGAATSPNA
                     NVYAYAAAQVKKALEVTHRLGGEGYTFWGGREGYATLWNTDIKRELDHLAAFLHMAVD
                     YKKKIGFKGPFYIEPKPREPSTHQYDSDAAACLNFLREHGLMEHFKLNLETNHATLAG
                     HSMWHEMEVAVAAGALGSVDANTGDELIGWDTDQFPTDIYLTTQMMLVLLRSGGFTTG
                     GLNFDAKVRRESFEPVDLFHAHIGGMDAFARGLKIAAAILEDGRWEGFRKQRYATFDK
                     GIGAKIEARATSFEELEAYTLENGEPLIASGRQEMLENLINEFI"
     gene            1923..2924
                     /locus_tag="PLP58_16590"
     CDS             1923..2924
                     /locus_tag="PLP58_16590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009961499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HRJ10197.1"
                     /translation="MKQTHPSAPVFPFELIRADLEVVEKSIRDQARAFDPAVEGYVSY
                     VCQSSGKRIRPALALVAGGATGGKTAEHTRLSVILEMVHIASLVHDDIMDGAATRRGL
                     PTAAAKWGSALSVLLGDALFARALELATEFEDTHVCRSIARASRDVCSGEILQTQRRF
                     DLNLSVPDYLRMIEMKTAALFAAAAGLGARLNGVPESVERALHSYGMKLGTVYQIYDD
                     CLDLVGDEAKAGKTLRTDLAKGKLTLPVLHLLMEATDAQKQKLNKMLLKGEPMDTTLL
                     AGIADYEGAIEKAVRFAQVMLDDARAELVCLAGSPYKSAFEDIAAYLRHLLDQCRAA"
     gene            2973..4319
                     /locus_tag="PLP58_16595"
     CDS             2973..4319
                     /locus_tag="PLP58_16595"
                     /inference="COORDINATES: protein motif:HMM:NF012993.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein"
                     /protein_id="HRJ10198.1"
                     /translation="MKHILPLLGLLAPVPLTGAEWQFHAPRPETAPLHELHDGGGPAG
                     QPILTLQTGPGEHWAGHWTRSQPVEGGRHYRFTAWRKYENVPEPRRSVVARVIWQDEN
                     GRPVTWEKPATAGYAKGTVPRAEPEYPRDRGSAPGAWGRFEENLRAPIAARRARIELH
                     LQWAASARASWSQVSLVPIEPPPPRIVRLATAHLQPREGREPADKPPQFAPLIKEASR
                     QKVDLLVLPETLTFYGTGKAMHECAETIPGPSTDYFCAQAKLHRLHLVAGLVERDGPT
                     IYNTAALIGPDGSLIGKYRKVALPRSEIEAGLTPGRDYPVFDTAFGKVGMMICYDGFF
                     PEVARALAIQGAEVIAWPVWGCNPRLAAARACENHVHLISSTYTDSSKHDWMISGIWD
                     HYGDVIAHAKEWGSIAVAEVNLDERAHWNSLGDFKAQIPAHRPPALGEKQAMTSPP"
     gene            complement(4418..5419)
                     /gene="dusB"
                     /locus_tag="PLP58_16600"
     CDS             complement(4418..5419)
                     /gene="dusB"
                     /locus_tag="PLP58_16600"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009961419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HRJ10199.1"
                     /translation="MLPWLQPGCFPLYLAPMAGVTDAVFRALCKELGADVMVTEFVSA
                     EGILQRDDRTRHYTEFDDAQRPLGVQLFGADGRRMGEAARKIIDWKRPDFIDINFGCP
                     VNKVVSKNGGSALLKDCPLLASVAREIVRAVPVPVTAKMRTGWDAGHINAVEVARILE
                     DCGIQAIAVHGRTRAQGYSGLADWEVISEVAGAVKIPVIGNGDIATGADVEVRRAQTG
                     VAGVMIGRAAMANPWVFQEAKHYLATGAHLPPATAEARFALMRRHCVMALARGSHNGR
                     GGEFEIMRSLRGRLMHYTRGLPGGKHLRASLGHLGSLMELDDILAGWSPQPVLECGL"
     gene            complement(5452..6279)
                     /locus_tag="PLP58_16605"
     CDS             complement(5452..6279)
                     /locus_tag="PLP58_16605"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HRJ10200.1"
                     /translation="MTAPVPLPQQSALKKLRPAFIQEQDFAARQYRGRRENQEDYYAF
                     ADASEPGRPALDQVLLVVGDGLGGHAGGSVASYLAVNAFVRAFHEQPGAQGWRLRTAL
                     DTANETLGLVSARVPPTAAAMGTTLLALLAGQREAQWISVGDSPLFLFRQGALRRLNA
                     DHSLAPILEERVRAGSLSAEEAAAHPDRHALQSAVLGDTLRLVDFPAEPLPLEPGDIL
                     IAASDGIFTLTTRALEELLAFGKNTTADKIADAILFAIRRVNHERQDNTTVGVVKIH"
     gene            complement(6276..7385)
                     /locus_tag="PLP58_16610"
     CDS             complement(6276..7385)
                     /locus_tag="PLP58_16610"
                     /EC_number="5.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009961967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016857 - racemase and epimerase activity,
                     acting on carbohydrates and derivatives [Evidence IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldose epimerase family protein"
                     /protein_id="HRJ10201.1"
                     /translation="MHHRALLFSAALLLAASPAMISAAITSEVWGKTAAGEEVRLFTL
                     KNSTGMEARITNYGGIIVSLTAPDREGRFADVVLGFDTLEPYLGKHPHFGCITGRYAN
                     RIGGASFVIDGVEYQVTANSGKNHIHGGPQGFDKKVWKAAVDEAKNALVLTHTSPDGD
                     EGFPGTLECSVTYSLGADNALRIEYRATTDKPTVVNLTNHSYFNLGGEGSGDILGHEM
                     RISAESFTPTDDSLITTGEIASVIGTPLDFTQPRKIGERIEADFKPLIQGRGYDHNYV
                     LKGSGLRPAARVREPGGGRVMEVLTTDPGVQLYTANHLKGVPGKKGHVYAARHGFCLE
                     TQKFPDSPNKPQFPSAVLRPGQIYEHITIFKFSAE"
     gene            complement(7488..9554)
                     /locus_tag="PLP58_16615"
     CDS             complement(7488..9554)
                     /locus_tag="PLP58_16615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009960698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HRJ10202.1"
                     /translation="MKLTTLAAFILPSLSAAAAGHDVVIYGGTSAAITAAVQVKQMGK
                     SVIIVSPDKHLGGLTSGGLGWTDSGNKAVIGGLSREFYHRVYLHYQKPEAWRQQKQEE
                     YGNKGQGTAAMDGEARTMWIFEPHVAENIFDAWVRELDIPVVREAWLDREKGVKKEGG
                     RIVSITTLDGRTYEGKMFLDATYEGDLLAAAGVDYHVGREANSVHGEEWNGVQVGVLH
                     HKHHFGAVEKPVSPYKVPGDPNSGVLARISTEHPGVKGEGDHRVQAYCFRSCYTNDPS
                     NRVPFPKPEGYDASQYELILRIFDAGWREFFHKFDPIPNHKTDTNNHGPFSFDNIGYN
                     YDYPEASYERRREIIKEHETYQKGLMWFVANDPRVPEDVRAELQKWGLPKDEFTDNGN
                     WSHQLYIREARRMVGHYVMTENELRKKKPTPESVGMGSYTIDSHNVQRYITPEGYVQN
                     EGDIGVSTNGPYEIAYGSLVPKEGQGSNLLVPVAMSASHIAYGSIRMEPVFMILGQSA
                     ATAAVLAIEDGLAVQKVPYAKLRERLLKDGQILEFKAPPSPNAIQLEGIVVDDAKASL
                     GGHWQESRAGKHYGEGYQHDGNAGKGESLAVFQPALPKAGLYEVRVAYPANNNRASNV
                     PVTIFHNGGETKKVISQKPPGPVGGVAVSLGEYEFSPDRRPQVVIGNEGTDGYVVIDA
                     VQWIAK"
     gene            complement(9591..>10589)
                     /locus_tag="PLP58_16620"
     CDS             complement(9591..>10589)
                     /locus_tag="PLP58_16620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009964001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HRJ10203.1"
                     /translation="RTLRRLLEAQGGGAWGRHRGRRLEGDSFVCDESSMVDTPLMHHF
                     LAALPEDAHLLLVGDADQLPSVGPGMVLHDLIASGAVPCVRLTEIFRQAAESRIITSA
                     HDINQGRVPDLRPSRASDFFFLEESDPDAIRETLVQLARTRLPAKYGFDPVSDIQVLT
                     PMNRGPLGTRALNEALQNALNPPNEIKYEIERFGTTFRAGDKVIQTRNNYEKEVFNGD
                     IGHISAIETEPLKVSVRFEGGRLVDYEPGELDELQPAYALTIHKSQGSEFPCVLMPVS
                     TQHFVMLERSLLYTAVTRARRLVVLVGDPKALEMAVKKQSSRRRWTGLREALGSGS"
BASE COUNT         1784 a         3636 c         3284 g         1885 t
ORIGIN      
        1 cttcgcgctg gacgaagccc ccgccgaagc acccgtgaaa cccatcgcgg aatgggcgcc
       61 gaaacgctga gaattcaccg ggacattcat ccccggataa tcaaagaggc aggccgtttg
      121 gcctgcctct tcgatttcca gaactgcggg cagcctcaga tgaactcgtt gatgaggttt
      181 tccagcatct cctgacggcc ggaagcgatg agcggctcgc cattctcaag cgtgtatgcc
      241 tccaattcct cgaagctggt tgcgcgcgcc tcgatcttcg cgccaatgcc tttgtcaaaa
      301 gtggcgtaac gctgcttgcg gaaaccctcc cagcgcccgt cctccaggat ggcggcggcg
      361 attttcagcc cgcgggcgaa cgcgtccatg ccgccaatgt gcgcatggaa aaggtccacc
      421 ggctcgaagg actcgcgcct caccttggcg tcaaagttca ggccgcccgt ggtgaagccg
      481 ccgctgcgca gcagcacgag catcatctgc gtggtgagat agatgtccgt gggaaactgg
      541 tccgtgtccc agccgatgag ttcgtcgccc gtgttcgcgt ccacggagcc aagcgcgccg
      601 gcagccacgg ccacctccat ctcatgccac atgctgtggc cggccagggt ggcgtggttc
      661 gtttccaggt tcagcttgaa atgctccatg aggccgtgct cgcgcaggaa gttcaggcag
      721 gcggcggcgt cgctgtcata ctggtgggtg gagggctcgc gcggcttcgg ctcgatgtag
      781 aaggggcctt tgaagccgat tttctttttg taatccaccg ccatgtgcag gaaggcggcc
      841 aggtggtcca gctcgcgctt gatgtcggtg ttccagagcg tcgcataacc ctcgcggcca
      901 ccccagaagg tgtagccctc gccgccgagg cggtgggtga cctcaagggc cttcttcacc
      961 tgcgcggcgg cgtaagcgta aacattcgcg ttcgggctcg tggccgcgcc ttggttgtag
     1021 cgcggatgcg cgaacaggca tgcggtgccc cagagcagct tcttgcccgt ggccttctgc
     1081 gccttttcca actcgtctgc cacggcgtcg aggttggcat tgctctccgc cagggtctcc
     1141 ccctcgggtg ccacatcgcg gtcgtggaag cagtagtagt ccacaccgag tttgtccatg
     1201 aactcgaagc aggcctgcac gcggcgctgc gcgttggcga tggaactggt gccgtcatcc
     1261 cacgggcgct gcgcggtgcc cacgccgaaa ggatcggcca gactgttgcg catggcgtgc
     1321 cagtaggcga tgccgaagcg gagatgctcg cgcatggttt tgccggcgac gacctcgtcc
     1381 gcgttgtagt gcttgaagga aagggggttt ttggatttcg gaccctcgta ttggatcctc
     1441 ggaatgtcag ggaaggcttc gttcatgatg gagaggccgc attggaaccc gccgcccgtt
     1501 gagcgtcaag ccccaaggcc tgacgaattc tgtggcgaat cggacacccg cttccgatcc
     1561 cacccaaaaa aagcggcccg gcgggaacca accattttcc cgccgggcct tgatttgacc
     1621 aagctgtccc caaccatgaa gtaacagccc ggctcgttgt ggtgaagctc gcgcttcgga
     1681 aagtgaaaag agcagccgcc gggccaagtc agagcgggaa tttctccaga gatgaaaatt
     1741 tttcaccgat gcctcccgcc ccgttcatgg tcgccgccgc ccgcgtggct ttcacaaaaa
     1801 tcaatctctg tgaaagatca cccgaggaat ccgggcaggt cgtaattgac tcaaaggagc
     1861 ggctgcgcga actaggaccg caacttctcc ccgcgaaggc gccgcccgca aaccgcctga
     1921 acatgaagca aacccacccc agcgccccag tatttccgtt tgaactcatc cgcgccgacc
     1981 tcgaagtcgt ggaaaagtcc atccgggatc aggcgcgcgc cttcgacccc gccgtcgaag
     2041 gctacgtcag ctatgtgtgc cagtcctcgg gcaagcgcat ccgtcccgcg ctagccctcg
     2101 tggcaggcgg ggccaccggc ggcaaaaccg cggagcacac acgcctttcg gtcatccttg
     2161 aaatggtcca catcgcctcc ctggtgcatg acgacatcat ggacggcgcc gccacacggc
     2221 gcggcctgcc caccgccgcc gccaagtggg gcagcgcgct gagcgtgctg ctcggtgacg
     2281 ccctctttgc ccgcgcgctg gagctggcca ccgagttcga agacacgcat gtctgccgca
     2341 gcatcgcccg cgcctcgcgc gatgtgtgca gcggggagat tctccagacc cagcgccgtt
     2401 ttgatctcaa cctctccgtg ccggactacc tgcgcatgat tgaaatgaag accgccgcgc
     2461 tcttcgccgc ggcagcagga cttggcgcgc gcctcaacgg tgtgccggaa agcgtcgagc
     2521 gcgccctgca cagctatggc atgaagctcg gcaccgtcta tcagatctac gatgactgcc
     2581 ttgacctcgt gggagatgag gccaaggcag gcaaaaccct gcgcaccgac ctcgccaagg
     2641 gcaagctcac gctgcccgtc ctccatcttc tcatggaggc caccgatgcg cagaaacaga
     2701 aactcaacaa aatgctcctc aaaggcgagc caatggacac caccctgctc gccggcatcg
     2761 ccgactacga gggcgccatc gaaaaggccg tccggttcgc ccaggtcatg cttgatgacg
     2821 cccgtgctga gctcgtctgc ctggcaggct cgccatacaa gtccgccttc gaggacatcg
     2881 ccgcctactt gcgccatctt ctggaccaat gccgcgccgc ctgacgcgcg cgccagcaag
     2941 gccgcacggc ccgcagtcgt atcctgcgcg gcatgaaaca catccttccc ctcctcggtc
     3001 tcctggcccc agtcccgctc accggagccg aatggcaatt tcacgcgcca cgccccgaga
     3061 cagcaccgct ccacgagctt catgacggcg gcggacccgc ggggcagccc attcttaccc
     3121 tgcaaaccgg gccaggcgag cactgggccg gccattggac ccgcagccag cctgtcgaag
     3181 gcggccgcca ctaccgcttc accgcctggc ggaaatacga aaacgtcccc gaaccccgcc
     3241 ggagcgtcgt cgcccgcgtc atctggcagg atgaaaacgg cagacccgtc acctgggaaa
     3301 agcccgccac cgccggttac gctaaaggca ccgtcccacg cgccgagccc gaatacccgc
     3361 gcgaccgagg ctccgctccc ggtgcatggg gccgcttcga ggaaaacctc cgcgctccca
     3421 tcgccgcccg ccgcgcccgc atcgaacttc acctccaatg ggcagccagc gcgcgcgcca
     3481 gttggagcca ggtctcactt gttcccatcg agccgccgcc gccgcgcatc gtccgtctcg
     3541 ccaccgccca tctccagccg cgcgaagggc gagagccagc cgacaagcca ccccagttcg
     3601 ccccgctgat caaagaagcc tcacgacaga aagtggacct cctcgtcctg cccgagaccc
     3661 tcacttttta tggcaccggc aaagccatgc acgaatgcgc cgagaccatt cccggcccca
     3721 gcaccgacta cttctgcgcg caggcaaaac tgcatcgtct tcatctcgtc gccggactcg
     3781 tcgaacgcga cggacccacc atttacaaca ccgctgccct catcggcccg gatggctccc
     3841 tcattggcaa ataccgcaaa gtggccctgc cccgcagcga gatcgaggca ggcctcaccc
     3901 ccggccgcga ctaccctgtc ttcgacaccg ccttcggcaa agtcggcatg atgatctgct
     3961 acgatggctt cttccccgaa gttgcccgag ctctcgccat ccagggggcc gaagtgatcg
     4021 cctggcctgt ctggggatgc aatccccgcc ttgctgccgc ccgggcctgt gagaaccacg
     4081 tccatctcat tagcagcacc tatactgaca gctcaaagca cgactggatg atctccggga
     4141 tatgggacca ctacggcgat gtcatcgcgc acgccaaaga gtggggcagc atcgccgtcg
     4201 ccgaggtcaa cctcgatgag cgcgcccact ggaacagcct cggcgacttc aaagcccaaa
     4261 tccccgccca ccgccctccc gccctcggcg agaagcaagc catgacatcg ccaccgtgat
     4321 tcagctcatg aggctggagc cggacgaaaa ccatcccgcg ccagtgttgc tgtcggagtg
     4381 atccttcggg ggccacgaaa gctctcagtc gcgccagtca aagcccgcac tccagcaccg
     4441 gctgcggaga ccacccggcc aggatgtcat ccaactccat caggctgccc agatgaccga
     4501 gggaggcgcg caggtgcttg ccgccgggca ggccgcgcgt gtagtgcatg agccgcccgc
     4561 gcagggagcg catgatctcg aactcgccgc cccgcccgtt gtgcgagccg cgcgccagcg
     4621 ccatcacaca atgccggcgc atgagggcga agcgcgcctc cgccgtcgcc ggcggcaggt
     4681 gcgcgccagt ggccaggtag tgcttcgcct cctgaaagac ccacgggttt gccatggccg
     4741 cgcgcccgat catgacaccg gccacgcccg tctgcgcgcg ccgcacctcc acgtccgcgc
     4801 cggtggcgat gtcgccattg ccgatgacgg ggattttcac cgccccggcc acctcgctga
     4861 tgacctccca gtcggccaga ccactgtaac cctgcgcgcg ggtgcgcccg tggacggcga
     4921 tggcctggat gccgcagtcc tccagaatgc gcgccacctc gacggcgttg atgtgcccgg
     4981 cgtcccagcc ggtgcgcatc ttggccgtga caggcacggg cacggcgcgg acgatctccc
     5041 gcgccacgct tgcgagcaag gggcagtcct tgagcaaagc ggagccgccg tttttcgaga
     5101 cgaccttgtt caccgggcag ccgaagttga tgtctatgaa gtcgggccgc ttccagtcta
     5161 tgatcttgcg cgcggcctcg cccatgcggc gtccgtccgc gccgaagagc tgcactccca
     5221 gcgggcgctg cgcgtcgtcg aactcggtgt agtgccgcgt gcggtcgtcg cgctggagga
     5281 tgccttcggc gctgacgaac tcggtgacca tgacatcggc gcccagctcc ttgcagagcg
     5341 cgcggaagac ggcgtccgtc acccccgcca tcggggcgag gtaaagcgga aaacagccgg
     5401 gctggagcca gggaagcatg gcgggaggat acggcgcgcc ggacgcgcgg gctaatgaat
     5461 cttcaccact ccgacggtgg tgttgtcctg ccgctcatgg ttcacgcggc ggatggcgaa
     5521 aagaatggca tcggcgattt tgtccgccgt ggtgtttttg ccaaaggcca gcagctcctc
     5581 cagagcgcgg gtggtcaggg tgaaaatgcc gtcgctggcg gcgatgagga tgtcccccgg
     5641 ctccagcggc agcggctcgg cggggaagtc cacaaggcga agagtgtccc ccagcacggc
     5701 gctctgcagc gcgtgcctgt cggggtgcgc ggcggcctcc tcggcgctga gactgccggc
     5761 gcgcacgcgc tcctccagga tgggcgcgag ggagtggtcc gcgttcagcc tgcgcagggc
     5821 gccctggcgg aaaagaaaga gcggcgagtc gccgacgctg atccactggg cctccctctg
     5881 cccggccagc aaagccagaa gggtggtgcc catggccgcg gcggtggggg gcacgcgcgc
     5941 gctgacgaga cccagcgtct cattggcagt gtccagcgcg gtgcgcaggc gccagccctg
     6001 cgcgccgggc tgctcgtgaa aagcgcgcac aaaggcgttc accgccaggt agctcgccac
     6061 gctgccgccg gcgtgcccgc cgaggccgtc gcccaccacc agcagcacct ggtccagcgc
     6121 cgggcggccc ggctcggagg cgtcggcaaa ggcgtagtag tcctcctggt tctcgcgccg
     6181 cccgcggtac tgccgggcgg cgaagtcctg ctcctggatg aaggcagggc ggagtttttt
     6241 gagggcggat tgctgcggca gggggacggg cgcggtcact cggcggaaaa cttgaagatc
     6301 gtaatgtgct cgtagatctg cccgggccgc agcacggcgc tggggaactg cggcttgttc
     6361 gggctgtcgg ggaatttttg cgtctccagg cagaagccgt gccgcgcggc atagacatgg
     6421 cccttttttc ccggcacacc cttgagatga ttggcggtgt aaagctgcac gccggggtcg
     6481 gtggtgagca cctccatgac gcgcccgccg cccggctccc tgacgcgcgc ggcgggccgc
     6541 agcccggagc ctttgagcac atagttgtga tcgtagcccc tgccctggat gagcggcttg
     6601 aaatccgcct cgatgcgctc gccgatcttg cgcggctgcg tgaagtccag cggcgtgccg
     6661 atgacgctgg cgatttcccc cgtagtgatg agggagtcgt ccgtgggtgt gaatgactcg
     6721 gcactgatcc gcatttcatg accgagaatg tcaccggagc cttcgccgcc gaggttgaag
     6781 tagctgtggt tggtcaggtt caccaccgtg ggcttgtccg tggtggcgcg gtattcgatc
     6841 ctcagggcgt tgtccgcgcc caggctgtag gtcaccgagc actccagcgt gccggggaag
     6901 ccctcgtccc cgtccgggct ggtgtgggtg agcacgaggg cgttttttgc ctcgtccacc
     6961 gccgccttcc acaccttctt gtcaaagccc tgcggcccgc cgtggatgtg gtttttgccg
     7021 gagttcgccg tcacctggta ctccacaccg tcaatgacga acgaggcccc gccgatgcgg
     7081 ttggcgtagc gcccggtgat gcagccgaag tgcggatgct tccccagata gggctccagc
     7141 gtgtcaaagc caagcaccac atccgcgaaa cgcccctcgc ggtccggcgc ggtgagggag
     7201 acgatgatgc cgccgtaatt cgtgattcgc gcctccatgc cggtgctgtt tttcagcgtg
     7261 aaaagccgca cctcctcgcc ggcggcggtc tttccccaga cttcggaggt gatggcggcg
     7321 gagatcatgg cggggctggc ggcaagcaga agcgcggcgg aaaagaggag cgctcggtgg
     7381 tgcatggaag gtgtcggggt tggcaggccg gaagtctggc ggctgcgcct catgcttgca
     7441 accgccgatt cacacccgaa tcaccgcaaa gaccgctccc acgctgctta tttggcgatc
     7501 cactgcacgg cgtcaatgac cacatagccg tcggtgccct cgttgccgat gaccacctgc
     7561 gggcggcggt ccggggagaa ttcatactcg cccagcgaca ccgccacgcc gccgaccggc
     7621 cccggtggct tctggctgat gaccttcttc gtctcgccgc cattgtggaa gatggtgacc
     7681 ggcacgttgc tggcgcggtt gttgttcgca ggataggcca cgcgaacctc atagagcccg
     7741 gccttgggca gcgcgggctg gaaaacggcc aggctctcgc ccttgccggc attgccgtcg
     7801 tgctgatagc cctcgccgta atgcttgccg gcgcggcttt cctgccaatg gccgccgagc
     7861 gaggccttgg catcatccac cacgatgcct tcgagctgga tggcgttcgg ggacggcggc
     7921 gctttgaatt ccaaaatctg gccgtccttc agcaggcgct cgcgcagttt cgcatacggc
     7981 accttctgca ccgccaggcc atcctcgatg gccagcaccg ccgccgtcgc cgccgactgg
     8041 cccaggatca tgaacaccgg ctccatgcgg atgctgccgt aggcgatgtg gctggcggac
     8101 atcgccaccg gcaccagcag gttgctgccc tgcccctcct tcggcaccag cgagccgtag
     8161 gcgatctcat acggaccgtt cgtgctgacg ccgatgtcgc cctcgttctg cacataaccc
     8221 tccggcgtga tgtagcgctg cacgttgtgg ctgtctatgg tgtagctgcc catgcccacg
     8281 gactcgggtg tgggcttctt tttgcgcagc tcgttttccg tcatcacata gtggccgacc
     8341 atgcgccgcg cctcgcggat gtagagctgg tgggaccagt tcccgttgtc ggtgaattca
     8401 tccttcggca ggccccattt ctgcagctcc gcgcgcacat cctccggcac ccgcggatcg
     8461 ttggccacga accacatcag gcccttctga taggtctcgt gctccttgat gatctcccgc
     8521 cgccgttcgt agctggcctc ggggtagtcg tagttgtatc caatgttgtc gaagctgaac
     8581 gggccgtggt tgttcgtgtc cgtcttgtgg ttcgggatgg ggtcgaactt gtggaaaaac
     8641 tcgcgccagc cggcgtcgaa gatgcgcagg atcagctcat actggctggc atcgtagccc
     8701 tccggcttgg gaaaggggac gcggttgctt ggatcgttcg tgtagcagga gcggaagcag
     8761 taggcctgca cgcggtggtc gccctcgcct ttgacgccag gatgctccgt gctgatgcgc
     8821 gccagcacgc cgctattcgg gtcgcccggc accttgtagg ggctgacggg cttctccacg
     8881 gcgccgaagt ggtgcttgtg atgcagcacg cccacctgga cgccgttcca ctcctcgcca
     8941 tgcacgctgt tggcctcgcg tcccacatgg taatccaccc cggcggcggc caacaggtcg
     9001 ccctcatagg tcgcgtccag gaacatcttc ccctcatagg tcctgccatc cagcgtcgtg
     9061 atcgagacga tgcgcccgcc ctcttttttc acgccctttt cgcggtcgag ccaggcctcg
     9121 cgcaccacgg ggatgtccag ctccctgacc catgcatcga agatgttctc cgccacatgc
     9181 ggctcaaaga tccacatcgt gcgcgcctcg ccatccatcg ccgccgtgcc ctggcccttg
     9241 ttcccatact cctcctgctt ctgctgccgc caggcctccg gcttctggta gtgcagatag
     9301 acgcggtggt aaaactcgcg cgacagcccg ccgatcaccg ccttgttgcc gctgtccgtc
     9361 cagcccagcc cgccgctcgt cagcccgccc agatgcttgt ccgggctgac gatgatgacg
     9421 gacttgccca tctgcttcac ctgcaccgcc gccgtgatgg ccgcgctcgt gccgccgtag
     9481 atgaccacat catgcccggc ggcggcggca gacaatgacg ggagaatgaa cgcggcaagg
     9541 gtggtgagtt tcatgcccac atccctgccc ggatggcctg ctttgtcaat tcaggacccg
     9601 ctgcccagcg cctctcgcaa ccccgtccag cgcctgcggc tcgactgctt tttgaccgcc
     9661 atctccagcg ccttcggatc gccgacgagc accaccagcc gccgcgcgcg agtcacagcc
     9721 gtgtacagca gactgcgctc cagcatcacg aaatgctgcg tgctcaccgg catcagcacg
     9781 cagggaaact cgctgccctg gctcttgtga atggtcagcg cgtaggccgg ctgcaattca
     9841 tccagctccc ccggctcata gtccaccagg cggcctccct cgaagcgcac gctcaccttc
     9901 agcggctccg tctcgatggc cgaaatgtgg ccgatgtcgc cgttgaagac ctccttctcg
     9961 tagttgttcc gcgtctggat caccttgtcc cccgcgcgga aggtggtgcc gaagcgctcg
    10021 atctcatact tgatctcgtt cggcgggttg agggcgtttt gcagcgcctc attgagcgcg
    10081 cgcgtgccca gcgggccgcg gttcatcggc gtcagcacct ggatgtcaga caccgggtca
    10141 aagccatact ttgcgggcag gcgggtgcgc gcgagctgca cgagcgtctc gcggatcgcg
    10201 tcgggatcgc tttcctccag gaaaaagaaa tcgctcgcgc gcgaggggcg caggtccggc
    10261 acgcggcctt gattgatgtc atgcgcgctg gtgatgatgc ggctttccgc cgcctggcgg
    10321 aaaatctccg tcagcctcac acagggcacc gcgccgctgg cgatgaggtc gtgcaacacc
    10381 atgcccggcc ccacggaggg gagctggtcc gcgtcaccca ccagcagcag atgcgcgtcc
    10441 tccggcagcg ccgccaggaa gtggtgcatc agcggcgtgt ccaccatcga ggactcatcg
    10501 cagacaaagg aatccccctc cagccgccgt ccgcggtgcc tgccccacgc gccgccgccc
    10561 tgcgcctcca gcagccggcg cagcgtgcg
//
LOCUS       DAOMYJ010000186        10508 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Prosthecobacter sp. isolate AMR_MDS_5629
            NODE_2105_length_10508_cov_19.395867, whole genome shotgun
            sequence.
ACCESSION   DAOMYJ010000186 DAOMYJ010000000
VERSION     DAOMYJ010000186.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867850
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prosthecobacter sp. (wastewater metagenome)
  ORGANISM  Prosthecobacter sp.
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Verrucomicrobiaceae; Prosthecobacter.
REFERENCE   1  (bases 1 to 10508)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10508)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.30
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:34:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,742
            CDSs (total)                      :: 4,684
            Genes (coding)                    :: 4,676
            CDSs (with protein)               :: 4,676
            Genes (RNA)                       :: 58
            tRNAs                             :: 55
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10508
                     /organism="Prosthecobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2105_length_10508_cov_19.395867"
                     /isolate="AMR_MDS_5629"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1965333"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1076
                     /locus_tag="PLP58_16625"
     CDS             <1..1076
                     /locus_tag="PLP58_16625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009963718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="terpene cyclase/mutase family protein"
                     /protein_id="HRJ10204.1"
                     /translation="EKLRQNGGSPECERAVSASLEWLKSQQNEDGSWGRNGGRSNKAA
                     MTGLALLCYLGRCETPDSPFYGDNVMRAIMYLIELSKKNQHGFITDDFKGNSGAYEHG
                     IATYALGEMYTLARLGSKELPGMRETFEKGVELIIKTQNGPDAGNGQGSWDYYTKNVA
                     EGSFTSKRPDLSVAGWQFQALKAAKYTGLKIQGLDGAIAKTVDYLERMQTKDGGFGGT
                     NRDSHYNQWSLTGVGALGLQTLSKGKTTVIKKGTKFLRDFLTAEPLDWEKNCNLYCWY
                     YYTQTFFQTGGDDWKFYNEQFLPQILAAQQPDGSFKRGRPNWPGGDAADPIYRQTLCT
                     LMLEVYYRYLKVGDREEQSFFDR"
     gene            complement(1119..2525)
                     /locus_tag="PLP58_16630"
     CDS             complement(1119..2525)
                     /locus_tag="PLP58_16630"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HRJ10205.1"
                     /translation="MKPLLLSITLITAAGQVTARPANLVFILTDNQGAWTLGCHGNKD
                     IRTPHIDRMAAEGLRFTRALSSNPVCSPTRATFLTGLMPSQHGVHSFLDPKYMMGPEA
                     YNTLEEFTSIGEVLRDAGYECGLSGKWHLGANLSPSEGFSFWITKPDGSTQEFYDQKI
                     IENGAVRDEAGYTTDLWTREGIRFIEQNKDRPFFLFLAYNGPYNLGKLMSNPPRNRHA
                     AYYKNKPFPSFPRDAMHPWQWANKQFHNTQTAMERCAAETSGVDDGVGEVMAALKRLG
                     LDDDTLVVYAADQGWMGGQNGMWGMGDHFRPTGAHDLMMHIPFILRHPARIPAGRSCD
                     LLVSNYDFMPTVLAHLGLADKMPAAPKSPGRDLSPVLAGQALEIADNAVFYEMETTRA
                     IRTDEWKYVARHPGGPFELYDMNADPHERFNIFGQPGTAMVKAELAARLDAFFNEFRD
                     PKHDIWKGGGSKAKRHTP"
     gene            2653..4017
                     /locus_tag="PLP58_16635"
     CDS             2653..4017
                     /locus_tag="PLP58_16635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009961375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="neutral/alkaline non-lysosomal ceramidase
                     N-terminal domain-containing protein"
                     /protein_id="HRJ10206.1"
                     /translation="MKLLSRLCPLILATLAHAQQNTEPGAAWKAGAASAVITPKKMIW
                     MGGYAARTKPAEGVEQDLHAKALVMEDDGGSKFALITMDLIGVPRDLRLAVAARVEKE
                     HGIPPARLAINASHTHSGPELRFKRLEGVDDPALRQAEAREYSAELENTLVSLTGAAL
                     KARAPAQIEFCEARCGFAMNRRTPDGKGGWRNFPNPAGPVDHQVPVLKVTGAGQKEIA
                     IVFGYACHCTTLGHQKFSGDYAGYAQEEIEKAHPGVVAMFMNGCSGDQNPYPRRKMEL
                     AQTHGKTLATAVESALGTTHRALSGRIRAAYREIPLAYGKLPSRAELEERSRSADKLD
                     AAHARRVLARLDEEGALPRHYPYPVQVIRLGDQLTWVTLAGEVVIDYALRLKRDIQDP
                     LVWVSAYTNDVPAYIPSRRVWEEGGYEGGDAMKWGHHPVPKWSPEVEQHIVGTVMELR
                     GGLD"
     gene            4069..4599
                     /locus_tag="PLP58_16640"
     CDS             4069..4599
                     /locus_tag="PLP58_16640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ10207.1"
                     /translation="MKTPLAAALVFFAALTASAQTSAPGMPANPNQEEVTRDGLWDGR
                     FKGGAYLVRCNQIIALSKHEYVADAAARVVEVNLTMNSNMTVRFYFLEPVKLEGGGVL
                     GAGQQALDRARAMVQDAASRVSPTIVEPKVVKNYPVSTHAHTVEFVVASEERLNSLHA
                     SLERSFRSGQGRIWRE"
     gene            complement(4605..5246)
                     /locus_tag="PLP58_16645"
     CDS             complement(4605..5246)
                     /locus_tag="PLP58_16645"
                     /inference="COORDINATES: protein motif:HMM:NF021759.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SprT-like domain-containing protein"
                     /protein_id="HRJ10208.1"
                     /translation="MLKPAQLFFDFQAGIRGSLAERALERPAYVEEDTPCTASAEPCV
                     SGRDEALTESCRSLLLGLGLDGAAGLVHALWNPRMRSTAGYARYPAWKIELNPRLREF
                     EGQVERTLRHELAHLVAYHRAGRRRIEPHGAEWRQACTDLGIPDETACHRLPLPRREV
                     RRRLTYSCAFCGLIVHRVKKFRRHTACRECCSKHNRGRYDSRYRFVLLSDAGK"
     gene            complement(5240..6190)
                     /locus_tag="PLP58_16650"
     CDS             complement(5240..6190)
                     /locus_tag="PLP58_16650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009958963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine acetyltransferase"
                     /protein_id="HRJ10209.1"
                     /translation="MTAQPMDCRQTEIVQSLMTSYHKVGGINHVDCGALPSKQAIASL
                     CEALLQLLFPGFFSEDAVSSQDLELITHEFVASIRERLKTEVRRSLRLRDGSGNHHEE
                     ATRLVCAFMERLPSVRELLQTDVQAAFEGDPAARSYEEIILAYPGLEAIAIQRAAHCL
                     YSAGVPLIPRMMTEWAHGRTGIDIHPGARIGSHFFIDHGTGVVIGETCEIGTRVKLYQ
                     GVGLVARSLAQGQALAGTKRHPTIGDNVTIYAGATIVGGDTIVGARSTIGANVFLMES
                     VPPDMIYALGDQEHRIRDRKKTPVTQSAKPASEASPNLPC"
     gene            complement(6337..6504)
                     /locus_tag="PLP58_16655"
     CDS             complement(6337..6504)
                     /locus_tag="PLP58_16655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ10210.1"
                     /translation="MMKTLLSILTIAALSSTAFAGDACKKCCADKGKTCVECCKDKGK
                     TCGKDCCKEAR"
     gene            complement(6680..6892)
                     /locus_tag="PLP58_16660"
     CDS             complement(6680..6892)
                     /locus_tag="PLP58_16660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ10211.1"
                     /translation="MGVLERGLSPEIATAPRSFIVALQAWEWRRTDILPGNPAACGMN
                     PALAAEHNAPPRPREPFYIRHCALLL"
     gene            complement(7173..10385)
                     /locus_tag="PLP58_16665"
     CDS             complement(7173..10385)
                     /locus_tag="PLP58_16665"
                     /inference="COORDINATES: protein motif:HMM:NF012789.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HRJ10212.1"
                     /translation="METAPAPLQHLLISASAGSGKTWQLVRRHLHLLALGVSPEDIAA
                     MTFTRKAAGEFFNRILRQLAELAVKPGAAADYFKDCSPPLAHEADFPGVLRRVTRRMH
                     RLRLGTLDSFFASVTTCFPLELGLGSTARVMDEKETELAARAALASLLEKLHREENAA
                     SMSQLMEAHKQATFGAEEKSADESLMDLTRKWLALWEESSGSNDSLRGWGDVSLPGMS
                     TPLTPVSDLIAKVRAHFIPPEKGAALLDETLAEALETVPCLSLPKRVRELLAKLAEVW
                     DDLPAGRAVIVWNRARLELSTQAGKALSELAEGIIARELHGRARRTRGIARIIGLYAE
                     EYAAQVRQRGRLSFADVQRLLARAAVEKSPWLGGGGDLWFRLDARHAHWMLDEFQDTS
                     RKQWQIVSNLVDEVIQDHGGRRSFFAVGDPKQSIYLWREAEPGLFDDILALYPARAGG
                     GLHQMPLSMSWRSAQPVLDMVNRVFGSRTLIEELLPAGCMDGFAFQAHEAAKKDLAGH
                     AALLSTPVVEKGAEQEALAGAAAGLLRGVDPLGRGLTCAILTRQNNEAAALAETLRAL
                     TGMDIVCEAETRPCTDNAATLALLSLLSLAAHPGDTAALEHLRMTPLWPLIESGGEGW
                     KLTALKTLALVQARGFAGFMEQWTPRLRGHLPELDAFHLRRLGQMADIAAEYDAGEGR
                     CVDGFLRFAREYPMRARGPARSIQVMTIHAAKGLEFDLVIQMAHDARSMRHVRDDKLL
                     ACREQGTVRWVLEAPPRACGRLVPELDSLLQEAEKRAAFEALCRLYVAMTRAIHGLYI
                     IAKPAPKKGESVNEGRLLRETLGSTPPRGHAQEYVIEWETGDPDWFRRRQPAAKPAVA
                     ATPPPAAPLGHLLRETQPIPRRRTPSGEETFRVKGSVLFSPGREPGRRLGTLVHELLA
                     GVEWRPDLRALESRWLSQGLLTPDDLAGGADAVAKEALRQARAVLAAPECAAAFTAPS
                     PHAIVWRERSFDLITEGGWVSGTFDRVLIDRDSAGRPLSAWIVDYKTDDISDDAKLES
                     KCQGYAPQIALYRQAIARLAGLPEEKVRASLLFTRLLKLV"
BASE COUNT         1848 a         3282 c         3503 g         1875 t
ORIGIN      
        1 tggagaagct gcgccagaat ggcggctcgc cggagtgcga gcgcgcggtg tccgcctccc
       61 tcgaatggct caaaagccag cagaacgagg atggctcctg ggggcgcaac ggtggcaggt
      121 ccaacaaggc ggcgatgacg ggcctggccc tgctttgcta cctgggccgt tgcgagacgc
      181 cggactcgcc cttctacggg gacaatgtca tgcgcgccat catgtatctg atcgagttgt
      241 ccaagaaaaa ccagcacggc ttcattaccg atgatttcaa gggcaattcc ggagcctacg
      301 agcacggcat cgccacctac gcgctgggcg agatgtacac gctggcacgc ctcggcagca
      361 aggagctgcc cggcatgagg gagacgtttg aaaaaggagt cgagctcatc atcaaaaccc
      421 agaacggtcc ggatgccggc aacggccagg gttcatggga ttactacacc aagaatgtcg
      481 cggagggcag ttttacctcc aagcgccccg acctttccgt ggctggctgg cagttccagg
      541 ctctcaaagc cgccaagtac acgggtttga aaattcaggg actggatggc gcgattgcaa
      601 aaacggtgga ttacctggag cgcatgcaga ccaaggacgg aggctttggc ggcaccaacc
      661 gcgactcgca ctacaaccag tggagcctca ccggcgtcgg cgcgctcggc ttgcagactc
      721 tgagcaaggg caagaccacg gtcatcaaaa agggcaccaa atttctgcgc gacttcctca
      781 ccgccgagcc gctcgactgg gagaagaact gcaacctcta ctgctggtac tactacaccc
      841 agaccttctt ccagaccggc ggtgacgact ggaagtttta caacgagcag ttcctcccgc
      901 aaatcctcgc cgcccagcag ccggacggca gcttcaagag gggccgtcca aactggcccg
      961 gaggcgacgc cgccgatccc atctaccggc agacgctttg cacgctcatg cttgaggtct
     1021 attaccgtta tttgaaagtg ggcgaccgcg aggagcagag cttcttcgat cgctgatcct
     1081 tccgtgcgcg gacatctctt tttggccgct cccgaaggtc agggtgtgtg ccgttttgcc
     1141 ttgctgccgc cgcccttcca gatgtcgtgt ttcgggtcgc gaaactcgtt gaaaaaggcg
     1201 tcaagacgcg ccgccagttc cgccttgacc atcgcggtgc ccggctggcc gaagatgttg
     1261 aagcgctcat gcgggtccgc gttcatgtcg tacaattcaa aggggccgcc agggtggcgc
     1321 gccacatatt tccactcgtc ggtgcggatg gcgcgcgtgg tctccatctc ataaaagacc
     1381 gcgttgtccg cgatctccag cgcctgtccc gcgagcacgg gcgaaagatc gcgtccgggg
     1441 gactttggcg cggcgggcat tttgtccgcc agaccaagat gagcgagcac ggtgggcatg
     1501 aaatcataat tgctgacaag cagatcgcat gagcggcccg ccgggatgcg cgccggatgg
     1561 cggaggatga agggaatgtg catcatcagg tcgtgcgcgc ccgtggggcg gaagtggtcg
     1621 cccatgcccc acatgccgtt ctggcctccc atccagccct ggtcggcggc atagaccacg
     1681 agtgtgtcgt catccagtcc gaggcgcttg agcgcggcca tgacctcgcc gacgccgtcg
     1741 tccacgccgc tggtttcggc ggcgcagcgc tccatggcgg tctgggtgtt gtggaattgc
     1801 ttgtttgccc actgccaggg gtgcatggca tcgcgcggga aactggggaa gggcttgttc
     1861 ttgtaataag cggcgtgccg gttgcgcggc gggttgctca tcagcttgcc gagattgtac
     1921 gggccgttgt aggccagaaa gagaaagaac ggtcggtcct tgttttgctc gatgaagcgg
     1981 atgccttcgc gtgtccagag gtccgtcgtg tagcccgcct catcccggac cgcgccattt
     2041 tcgatgatct tctggtcata aaactcctgt gtcgaaccat ccggttttgt gatccaaaaa
     2101 gaaaacccct cgctcggcga gaggttcgcg cccagatgcc acttgccgct caggccgcat
     2161 tcataaccgg catcgcgcag cacctcgccg atgctggtga actcctccag tgtgttatat
     2221 gcctccggtc ccatcatgta tttcggatcg agaaaggaat gcacaccatg ctgggagggc
     2281 atcagaccag tgagaaaagt cgccctggtt ggagagcaga ccggattgct gctcagcgcg
     2341 cgggtgaagc gcagcccctc cgccgccatc cggtcaatgt gaggagtgcg gatgtccttg
     2401 ttgccatggc atcccagggt ccacgctccc tggttgtccg tcaggatgaa gacgaggttt
     2461 gctggccggg ccgtgacttg gcctgcggcg gtgatcaggg ttattgagag aaggagcggt
     2521 ttcatgacca ggggtgaatg acggattttc aaaatgatca aacggccttc acgcccggag
     2581 tgttcctgga aagccacttc ggggcattcc tgaaaatgca aggaccgttc ttgcggcccc
     2641 gattgctccg ggatgaaact tctctcccgc ctctgccccc tcatcctggc caccctcgct
     2701 catgcccagc agaacaccga gcctggggcg gcatggaaag cgggggcggc cagcgcggtc
     2761 atcacaccga aaaagatgat atggatgggc ggttatgccg cgcgcaccaa gccggctgag
     2821 ggagtggagc aggaccttca tgccaaggcg ctcgtcatgg aggatgacgg tggatcaaaa
     2881 ttcgccctca tcaccatgga tctcatcggc gtgccgcgcg atctccgcct ggccgtggcg
     2941 gcgagggtgg aaaaagagca cggtattcca cccgcgcggc tcgccatcaa tgccagccac
     3001 acccacagcg gcccggaact gcgcttcaaa aggctggaag gtgttgacga tcccgcgttg
     3061 cgccaggccg aggcgcggga atacagcgct gaattggaga acacactcgt cagcctcacc
     3121 ggcgcggctt tgaaagcgcg cgctccggca cagatcgaat tctgcgaggc gcgctgcggc
     3181 ttcgccatga accgccgcac accggacggc aaaggcggct ggcgaaattt tccgaatccc
     3241 gccggaccag tggaccacca ggtgccagtg ttgaaagtga ccggtgccgg gcaaaaagaa
     3301 atcgccatcg tttttggcta cgcctgccac tgcaccacgc tcgggcacca gaaattcagc
     3361 ggcgactacg cgggttacgc gcaggaggag attgagaaag cgcatcccgg cgtcgtggcc
     3421 atgttcatga acggctgcag cggcgatcag aatccttatc cgcgccgcaa gatggagctg
     3481 gcgcagacgc atggcaaaac cctcgccact gccgtcgagt ccgcgcttgg caccacgcat
     3541 cgagccctga gcggccgcat ccgcgccgct taccgcgaga ttcccctggc ctatggcaag
     3601 ctgccctcgc gcgccgaact ggaggagcgc tcccgctccg ccgacaagct tgatgccgcg
     3661 catgcgcgcc gggtgctggc gcgtcttgat gaggagggtg cgctgccgcg gcactaccct
     3721 tatccagtgc aggtcatccg cctcggcgac cagctcacat gggtcaccct tgccggagag
     3781 gtggtcattg actacgccct tcgtctgaag cgggacatcc aggacccgct cgtctgggtc
     3841 tccgcctaca ccaatgacgt gcccgcctac atccccagcc gtcgtgtgtg ggaagaaggc
     3901 ggttatgagg ggggcgacgc catgaagtgg ggccaccacc ccgtgcccaa atggagcccc
     3961 gaagtcgagc agcacatcgt cggcacggtg atggagttac gaggcgggct ggattagctc
     4021 cattcacagg atttactttt caccctcaag gggcagtatt tcgcagccat gaaaactccc
     4081 ctcgctgccg cccttgtttt ttttgccgcc ctgaccgcct cagcgcagac cagtgcgccc
     4141 ggcatgcccg ccaatccgaa ccaggaggag gtgacgcggg acggtctttg ggatgggcgc
     4201 ttcaagggcg gcgcatacct cgtgcggtgc aaccagatca tcgcgctctc caagcatgag
     4261 tatgtggcgg acgcggcggc gcgggtggtg gaggtgaatc tgacgatgaa ctcgaacatg
     4321 accgtgcgct tttatttcct ggagccggtg aagctggagg ggggcggcgt gctcggcgcg
     4381 gggcagcagg cgctggaccg cgcgcgggcg atggttcagg acgctgccag ccgcgtgtca
     4441 ccgacaatcg tggagccgaa ggtggtgaaa aactatccgg tctccaccca tgcgcacacg
     4501 gtggagtttg tggttgcctc cgaggagagg ctgaactcgc tgcacgccag ccttgagcgc
     4561 tcctttcgct cggggcaggg gcgcatctgg cgcgagtgac ccggctactt gcctgcgtca
     4621 gacagcagca cgaaacggta gcgcgagtca tagcgtccgc gattgtgctt cgagcagcat
     4681 tcgcggcagg cggtgtgccg gcggaatttt ttcactctgt ggacgatgag gccgcagaat
     4741 gcgcatgaat aagtgagtct gcggcgcacc tcgcgacgcg gcagcggcag gcggtggcag
     4801 gcggtctcat cagggatgcc gaggtccgtg caagcctggc gccactccgc tccatgcggc
     4861 tcgatgcggc ggcgtcccgc gcggtggtag gcgacgaggt gcgccagttc atgacgcagc
     4921 gtgcgctcca cctggccctc aaactcgcgc agacgcgggt ttagctcgat cttccatgcc
     4981 gggtagcgcg cgtagccggc ggtgcttctc atgcgcggat tccacagcgc atgcacgagg
     5041 ccggcggcgc cgtcgaggcc aaggccgaga agaagtgagc ggcatgattc ggtgagcgcc
     5101 tcatccctgc cgctgacgca tggctccgcg gatgcggtgc acggggtgtc ctcctcgacg
     5161 tacgcgggcc gctccagcgc gcgctcggca agactgccgc ggatgccggc ttgaaagtcg
     5221 aagaagagct gggcgggttt cagcatggca ggttgggaga cgcctcggac gcgggcttgg
     5281 ccgactgggt gaccggggtt tttttgcggt cgcggatgcg gtgctcctgg tcgccgaggg
     5341 cgtagatcat gtctgggggc acgctttcca tgagaaagac attcgcgcct atggtgctgc
     5401 gcgcccccac gatggtgtct ccgccgacga tggtggcgcc tgcgtagatg gtgacgttgt
     5461 cgccaatcgt ggggtggcgc ttggtgccgg ccagagcctg cccctgggcc agcgagcggg
     5521 ccacgagtcc cacaccctgg taaagcttca cccgcgtgcc gatctcgcac gtttcgccga
     5581 tgacgacgcc ggtgccgtgg tctatgaaaa aatggctgcc gatgcgcgcg cctgggtgga
     5641 tgtctatgcc ggtgcggcca tgcgcccact ccgtcatcat gcgcgggatg agcggcacac
     5701 cggcggagta gagacagtgc gcggcgcgct gaatggcgat ggcctccagc cccggatagg
     5761 cgaggatgat ctcctcgtag ctgcgcgcgg cgggatcgcc ttcgaaggcc gcctgcacat
     5821 cggtttgcag cagctcgcgc acggagggca ggcgctccat gaacgcgcag acaagccggg
     5881 tggcctcctc gtggtggttg ccgctgccgt cgcgcaggcg caggctgcgg cgcacctcgg
     5941 ttttcagccg ctcgcggatg ctggccacaa actcatgtgt gatgagttcg aggtcctgcg
     6001 aggaaacagc gtcctcggag aaaaacccgg gaaaaagaag ctggagcagg gcctcgcaga
     6061 gcgaggcgat ggcctgcttc gagggcagag cgccgcagtc cacatgattg atgccgccga
     6121 ccttgtggta ggaggtcatg agactttgga cgatttcggt ctggcggcaa tccatgggct
     6181 gggctgtcat ttagagcaga atacgcgcgc tggcaaatgg cggagcgggc aaggagggcc
     6241 ggaccgcgtc tcctccgtgc cggcatgaaa aaccgccgtc aaccgcgggg cggatgacgg
     6301 cggccggagt gccctcaaac ctgggtttga gaacggttac ctggcctctt tgcagcagtc
     6361 cttgccgcag gtcttgcctt tgtccttgca gcactcgacg caggtcttgc ctttgtcggc
     6421 gcagcatttc ttgcaggcgt caccggcgaa ggcggtgctg gagagggcgg cgatggtgag
     6481 gatggagaga agtgttttca tcatgtgtga ttttcgatct tttgaagtgt tgctttgaga
     6541 tggtttacag aaacagcctg gatcatgcca ggcaggcgcg gcttcaggcc ggggtggtga
     6601 tggtccttcc gcaggagggt gcatgcgccc tttcgcgcgt gcgccgccat tcatgccacg
     6661 acgtgaatgc cgctgcttct caaagcagca gcgcgcaatg ccggatataa aacggctcgc
     6721 gcggtcgcgg cggggcattg tgctcagccg cgagcgccgg attcatcccg cacgcggccg
     6781 ggtttccggg caaaatatcc gtccgccgcc actcccacgc ctgcagggcg acgatgaacg
     6841 agcgcggtgc ggtggcaatt tctggagaaa ggccgcgctc aagaacgccc atgcagcagg
     6901 cgcaatcttt ccccggagtt ttgcccggcg gggcttcggt ctccccggcc agactctggc
     6961 aggcggggca ggcctcaatc tgggcagtgg ctgcgggcat ggactgtgcc agacaacacc
     7021 agcacatggg cactgccatc aggcatgccg tgagatagct ggtcagattc ttgatcatat
     7081 ccacagacta gaacgtgcgg tgccggcttt ccgttcgaag aaaaatccat gaaccctgcc
     7141 ggcggacgcg gcgcgcgcgg ttcttggcgg cgtcacacga gcttgagcag gcgtgtgaag
     7201 agcaggctgg cgcggacttt ctcctccggc aggcctgcga ggcgggcgat ggcctggcgg
     7261 tagagggcga tctggggcgc gtagccctgg cattttgact ccagcttcgc gtcgtcgctg
     7321 atgtcgtcgg ttttgtaatc cacgatccac gcgctcaggg ggcgtcctgc agagtcgcgg
     7381 tcaatgagca cgcggtcgaa ggtgccggag acccagccgc cttcggtgat caagtcgaac
     7441 gagcgctcac gccagacgat ggcgtgggga gagggcgcgg tgaaagcggc ggcgcactcc
     7501 ggcgcggcca gcacggcacg cgcctgccgg agcgcctctt tggccacggc atctgcgccg
     7561 cccgcgaggt cgtccggcgt gagcaggccc tggctcagcc agcggctttc gagcgcgcgc
     7621 aggtccggcc gccactcgac cccggccagc agttcatgga cgagagtgcc gaggcggcgt
     7681 ccaggctcgc ggccgggact gaaaagcacg ctgcctttga cgcggaaggt ttcctcaccc
     7741 gaaggcgtgc ggcggcgcgg gatgggctgc gtctcgcgca ggagatggcc gagaggcgcg
     7801 gcgggcggcg gagtcgcggc caccgccggc tttgccgctg gctggcgccg cctgaaccaa
     7861 tcgggatcac cggtttccca ttcgatgaca tattcctgtg cgtgcccgcg cggcggcgtg
     7921 ctgccaaggg tttcgcgcag aagtcttccc tcgttgacgg actcgccttt tttcggagct
     7981 ggcttggcga tgatgtatag gccgtggatg gcgcgggtca tcgccacata aagccggcag
     8041 agggcttcga aggcggcgcg tttttcggcc tcctgaagca gtgaatccag ctccggcaca
     8101 aggcggccgc aggcgcgcgg cggcgcctcc agcacccagc gcaccgtgcc ctgctcgcgg
     8161 caggccagca gcttgtcatc ccgcacatgc cgcatggagc gggcgtcgtg ggccatctgg
     8221 atgacgaggt cgaactccag ccccttggcg gcatggatgg tcatgacctg gatggagcgc
     8281 gccgggcccc gcgcgcgcat gggatattcg cgcgcgaagc gcaggaagcc gtccacgcag
     8341 cgcccttccc cggcgtcata ctcggcggcg atgtcggcca tctggccgag acggcgcaga
     8401 tgaaaggcgt ccagttccgg cagatgcccg cgcaggcgcg gagtccactg ctccatgaag
     8461 ccggcgaagc cgcgcgcctg cacaagcgcc agggttttca gcgcggtcag cttccagcct
     8521 tcgcccccgc tttcgatgag cggccagagc ggcgtcatgc gcagatgctc gagcgcggcg
     8581 gtgtcgcccg gatgcgcggc cagggaaagc agggagagca gggccagagt ggccgcgttg
     8641 tccgtgcagg ggcgcgtctc ggcctcgcag acaatgtcca ttccagtcag ggcgcgcagg
     8701 gtttccgcga gcgcggcggc ctcgttgttc tggcgcgtca ggatggcgca ggtgaggccg
     8761 cggcccagcg ggtccacccc ccgcagaagc ccggctgccg ccccggccag cgcctcctgc
     8821 tccgcgccct tttccaccac cggtgttgaa agcagggctg catggcctgc gaggtctttt
     8881 ttggccgctt cgtgggcttg aaaagcgaaa ccgtccatgc agcccgcagg cagcaattct
     8941 tcgatcaaag tcctggagcc gaacacccgg ttcaccatgt ccagcacggg ctgcgcgctg
     9001 cgccaggaca tgctcaacgg catctggtgc agccctcctc cggcgcgcgc ggggtagaga
     9061 gccaggatgt cgtcaaagag tcccggctcc gcctcacgcc agaggtagat ggactgcttg
     9121 ggatctccca cggcgaagaa actgcgcctg ccaccgtgat cctggatcac ctcgtccacg
     9181 aggttgctga cgatctgcca ttgcttccgg ctggtgtcct ggaactcgtc cagcatccag
     9241 tgcgcgtggc gcgcgtcgag ccggaaccag aggtcgccgc cgccgcccag ccagggtgac
     9301 ttttccaccg ccgcgcgcgc cagcagacgc tgcacatcgg cgaaggagag ccggccgcgc
     9361 tgccgcacct gcgcggcata ttcctccgcg tagagaccga tgatgcgcgc gatgccgcgc
     9421 gtgcgccgcg cgcggccgtg cagttcgcgg gcgatgatgc cctcggccag ctcgctcagg
     9481 gccttgcccg cctgcgttga gagttcgagg cgggcgcggt tccagacaat caccgcgcgc
     9541 ccggcgggca ggtcgtccca gacttcggcc agctttgcca gcagctccct aacccgcttg
     9601 ggcaggctaa ggcagggaac agtctccagt gcctcggcca aagtttcatc gagcagtgcc
     9661 gcgccttttt ccggcgggat gaaatgcgcg cgcactttgg cgatgagatc gctcaccggt
     9721 gtcagcggcg tgctcatgcc gggcaatgac acatcccccc agccgcgcag actgtcattg
     9781 gagccggagg actcctccca gagcgcgagc catttgcgcg tcaggtccat gagcgactcg
     9841 tcggcgcttt tctcctccgc gccgaaagtg gcctgcttgt gcgcctccat gagctggctc
     9901 atggaggcgg cgttctcctc ccggtgcagc ttttccagca gggaggccag cgccgcgcgc
     9961 gcggcgagtt cggtctcctt ctcatccatc acgcgcgcag tcgagccgag gcccagttcc
    10021 agcggaaaac aggtcgtcac cgaggcgaaa aaactatcca gcgtgcccag gcgcaggcgg
    10081 tgcatgcgcc gtgtcacccg gcgcagcaca ccgggaaaat ccgcctcatg cgcgaggggc
    10141 ggcgagcagt ccttgaaata gtccgccgcc gcgcccggct tcaccgccag ttcggcgagc
    10201 tggcgcagga tgcggttgaa aaactccccc gccgccttgc gcgtgaaggt catggccgcg
    10261 atgtcctccg gactcactcc cagcgccagc agatgcagat gccggcgcac gagctgccag
    10321 gtcttgccgg agcctgccga ggcggagatg agcaggtgtt ggagcggagc aggcgcggtt
    10381 tccatgcggc cttgtttacc actggatgaa agtggttgcc aaacactttg ccctgctcgt
    10441 ccgcgagtgc cgtagggcgc gcgcaaggtt ccttacgggc tggcgttgac cttcccagcc
    10501 atgccgcc
//
LOCUS       DAOMYJ010000187        10371 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Prosthecobacter sp. isolate AMR_MDS_5629
            NODE_2143_length_10371_cov_21.645308, whole genome shotgun
            sequence.
ACCESSION   DAOMYJ010000187 DAOMYJ010000000
VERSION     DAOMYJ010000187.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867850
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prosthecobacter sp. (wastewater metagenome)
  ORGANISM  Prosthecobacter sp.
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Verrucomicrobiaceae; Prosthecobacter.
REFERENCE   1  (bases 1 to 10371)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10371)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.30
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:34:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,742
            CDSs (total)                      :: 4,684
            Genes (coding)                    :: 4,676
            CDSs (with protein)               :: 4,676
            Genes (RNA)                       :: 58
            tRNAs                             :: 55
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10371
                     /organism="Prosthecobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2143_length_10371_cov_21.645308"
                     /isolate="AMR_MDS_5629"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1965333"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            188..811
                     /locus_tag="PLP58_16670"
     CDS             188..811
                     /locus_tag="PLP58_16670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009959140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HRJ10213.1"
                     /translation="MASSPKNPAASPPPEAINEFVGKRVKKLRAQRSWSLDDFAAASG
                     VSRSMLSEIERERANPTLTVAARIARAFGLTLQELLNGAEHAPARIQVIRGDDPAQIF
                     RSDKQCEIRTLSPLSLEKDFEFYQLTLRPGGALRSQPHFEGTREFLTVEKGAVHVESS
                     GDGMDLAAGDSATYAADTAHAILNTGKSAAVVFLVVIYTQPRSPAAR"
     gene            complement(818..1741)
                     /locus_tag="PLP58_16675"
     CDS             complement(818..1741)
                     /locus_tag="PLP58_16675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ10214.1"
                     /translation="MKKTESLVVLALLLALLLLECGARMFETSLSKDVAHIRSLPAEA
                     ARLRQAPAGTLKVLILGNSLARCGLDRALLARGLEAASRRPVAVSVMHPDGSRVEEWR
                     HGYRRYFDQTGSRPDLVLLCTGRLHLEDGLRDLDSVAAFYTSWADLPAFVRSEGLGFG
                     DTCQAAAARASALFAHRRRVQPLVFYNFMPGYEQTVNLVQKTRNASAPTTRASPSCSN
                     LASLATTIGASGGTLLILPVPLPESYELPALVKQTAVNAGARVLHASPALPPSRFPDG
                     YHLDEEGARIWTQALLDSPEWKAALAGMAAK"
     gene            complement(1761..3194)
                     /locus_tag="PLP58_16680"
     CDS             complement(1761..3194)
                     /locus_tag="PLP58_16680"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015049.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBOAT family O-acyltransferase"
                     /protein_id="HRJ10215.1"
                     /translation="MWFNSFEFWIFFALVALGCRLMRHAGQNRWLLLASYFFYGCYDW
                     RFLSLILFCTWVNFIAGRRIHEAACPRARRRWLTAGVTLSLAVLGVFKYLGFFTREAS
                     RLLMWAGLADHRWALEVLLPAGISFFTFQALSYTMDIYRAQTKPCRSFSDFALYISFF
                     PQLVAGPIERSSHLLPQVEQPRPRCDEARFRKGFYLVLSGLFLKVVIADNMAWLAGHV
                     FAASPSSLAGPEVLLGVYAFAFQIYGDFAGYSAIACGTACWLGFDLMTNFRRPYFALD
                     PRDFWQRWHISLSTWLRDYLYIPIGGNRGGGWGTARNLMLTMLLGGLWHGAAWTFIVW
                     GGIHGLWLGLHRVWLRVKKPVVTAETRPSAPWWLAGAKWFVTFHLVCLTWLAFRADSL
                     RHALALLGALGGKWEVTDFTLTCAGMTFFFLLPWLAYEFWLERRKDDEALLKSAWWWR
                     AGLYVYLVLMMICFPPPAPAEFIYFRF"
     gene            3395..4786
                     /locus_tag="PLP58_16685"
     CDS             3395..4786
                     /locus_tag="PLP58_16685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HRJ10216.1"
                     /translation="MEFRFALFFLFLYYIRPQDWVAGWAGFNIIKPLMVVWIAALYTT
                     RNRPVVPGLLRTPHDWMVLLYYAYVVWNAPDTKAAFMGFMPLAVFYALTVQSLVSWER
                     VLGYLKAWNFMLLGVAAMAVGSLYGLDLTGAVDVTARNAGRLSIGTWLHNNPNSLAHT
                     VIVAIPLSYLLYFYKGSAMGRLVIFPLCAALAAECAFHASSKGSFLVAGGLVVLIFVI
                     GRPLGIKVLALSAAATMGVSALSFLPRMSGMSSLSSNEGVQGRLMAWEMAKTVLDSSN
                     TGVGWKQFVAWVSWEGETFVKATHSSYVQIGADLGVYGLFIYLTALWTAQRSLLATHR
                     FTRHDRTREQCRRAGIVLVSAYAVSNWMINREYHSEYFLMIAVAAAIHRLCIYESRQE
                     SQPEPSRVAAMPVNRRSNPARKQHVSRQEEEPSWEDSPPSLKSGPDWKDAVAGVALTW
                     CVVEIWDYVLTSL"
     gene            complement(4794..6323)
                     /locus_tag="PLP58_16690"
     CDS             complement(4794..6323)
                     /locus_tag="PLP58_16690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009965398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase"
                     /protein_id="HRJ10217.1"
                     /translation="MRLISRLCLAALLCAAVFPAAAQERKNVLFIISDDLNNYLGCYG
                     DPRAKTPNIDKLAARGVRFERSYCTFPLCGPSRNSMLTGLYPNSNGILTNSQIFRQSI
                     PSHISLPQAFRQNGYFAARIGKLYHYNVPKSVGTDGHDDPGSWEMEINPAGVDRMEEE
                     PEIFTLTPGQFGGTLSWYASPKSDAHHTDGMIASEAEWVLERCAKKKDRPFFLAVGFF
                     RPHTPYVAPETPYFGWYPEAEMPVHPTVDQNPPEVPAAALGSYKKEQDKLTDELRRRC
                     AQSYYASISFMDAQVGRVVEALDKNGLAESTVIVFTSDHGYHMGEHGLYQKMSLFEGS
                     ARVPLLIVAPGVTKPGSVAATPVSHLDLYPTLAELCGVKAPANLQGQSLVPILKDPQE
                     KGRGWAITQVSRGGGPNRATVSRDKGSEGRRFFGYSLRTPRWRYTEWDEGREGSELYD
                     HDADPGELKNLAADPAHAATVAELSEKVRAAAKGTFPAEGVTPPVNKEGPLWAPNLTD
                     P"
     gene            6510..7169
                     /locus_tag="PLP58_16695"
     CDS             6510..7169
                     /locus_tag="PLP58_16695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ10218.1"
                     /translation="MSKKLKPGLFNASSPSIKFTDVLKAIENKGYALRARGEFDLNVV
                     GVRRTKIKVDAFNDVLFAFFPYQGQWQYQKYQITTLPGYTYLAERLGNSKGTAILVPG
                     HYKDKYEVRQHGSGGFAHQALCQKRTSGGYVSGIKVWRDDTLDAVPNPDTSKTYEGGG
                     INIHRASASDCTVRVADYSAGCQVFRCVAEFDAFMTTTRRARDAWGNTFSYTLLDEAD
                     L"
     gene            7196..8221
                     /locus_tag="PLP58_16700"
     CDS             7196..8221
                     /locus_tag="PLP58_16700"
                     /inference="COORDINATES: protein motif:HMM:NF019844.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0070064 - proline-rich region binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HRJ10219.1"
                     /translation="MSKKASKKTAAKKAAAPAATPSVSLPQLVVTASSLNVRRSPSLT
                     ATVTGSLSSGTVVDWLETSPDELWAKVRHGTLSGWSSRRYLAETQAPAPGGPYDEILR
                     IADTSAIAQYRWLDRGRAPRAYIRGMALAFARVYCKLSAGDPAAMEMAKANTGVADVD
                     ALAHYAQIFHEAGMNNESSGASTLRHLYVLLVGLGMRESSGKHCEGRDTTAGNVTAET
                     AEAGLFQTSWNARIRPDMTTIQPMVQVFNHYRANPQGFLEIFQEGVTCSPQNWHNHGT
                     GLGRTFQQLSKECPAFAAEFAAVGLRNRRRHWGPVNRREAEVRPECNTMLLRVQQAVD
                     SLQLCPV"
     gene            8235..8879
                     /locus_tag="PLP58_16705"
     CDS             8235..8879
                     /locus_tag="PLP58_16705"
                     /inference="COORDINATES: protein motif:HMM:NF017519.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YiiX/YebB-like N1pC/P60 family cysteine
                     hydrolase"
                     /protein_id="HRJ10220.1"
                     /translation="MNSLTHLTAAFAALLLLASCQTQPGVSGTAALQPGDIVFQDSSP
                     VSGQAEAIKKLTRSDWSHCGIYFKDSSGKGVVVDGNGLSKAVAWEKWRANGNGGRYAA
                     WRAVNPLTAAQISALRKAADHLDGRIYDKRFAWDDERIYCSEIIWKAWRAAFQEELCP
                     LQKLGDFDLASREARALIERPDSWGSLENALRHRDEPVVSPQKLTESALLRRIR"
     gene            8941..9936
                     /locus_tag="PLP58_16710"
     CDS             8941..9936
                     /locus_tag="PLP58_16710"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HRJ10221.1"
                     /translation="MGPGTGGADHFLAKDSGASAWSVAVTQKGNLPDEKLRGLVWRAP
                     GRPQGVIVCLHGLQTHSAWFAPVAADLRNAGWTVIAPDRRDSGMNRQQNMAPVKSAEQ
                     LFGDLSAHLEQAAAEAPGKPLILLGTSWGSNLATAYLVRRQDARVSGLIQLVPATQVR
                     KNLAPKFAERVLGPVLSVLSPRMPARPTFCDFHYLAGGRSQSSGKGPPAIVDRRAPLP
                     EPPALEDSNSKLACILKKDDSLLREPSIRTLRVGLVLAEMWRKKTSAIPDNTPILILT
                     AGHDQIMDNRGALAAFPQGRVTHRDMPAGHGVQITHPHWVSRHVLEWLRSAGKRP"
     gene            9985..>10371
                     /locus_tag="PLP58_16715"
     CDS             9985..>10371
                     /locus_tag="PLP58_16715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009958164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ10222.1"
                     /translation="MRFRSILALPFAAAVPVLAEPSARDVEFFEKKVRPILVERCYEC
                     HSAESGKSKGGLTLDSQPAILQGGDNGPALVAGDPGKSLLIEAVRYQKRELQMPPKSP
                     LPAAELRVLEEWVARGAPDPRTEPVAS"
BASE COUNT         1965 a         3292 c         3301 g         1813 t
ORIGIN      
        1 gcggcatgaa gccgggatgg ggcggaggca gcggctgggt ggctccgggg gcggacgggg
       61 aattttcggg attcataaat tcaaagggtg ctggcaggaa taacaaacat cgcctctgga
      121 tggcaagaaa atatacggga ggcggctttc cactttacag gatgctcatc cggcatttcg
      181 aatgcgcatg gcctcctccc caaaaaatcc cgccgccagc ccgccgccgg aggcgatcaa
      241 tgaattcgtc ggcaagcgtg tgaaaaagct gcgcgcgcag cgctcctggt cgctcgacga
      301 tttcgcggcg gccagcggag tcagccgttc gatgctcagc gagatcgagc gcgagcgcgc
      361 caacccaacc cttactgtgg cagcgcgcat cgcgcgcgct tttggcctga ctttgcagga
      421 gctgctcaac ggcgcggagc acgcgcccgc gcgcatccag gttattcgcg gggatgatcc
      481 ggcgcagatt ttccgcagcg acaagcagtg tgaaatacgc acgctctccc cgttgagcct
      541 ggagaaggac tttgaattct accagctcac cctgcgtccg ggcggcgctc tgcgcagcca
      601 gcctcatttc gagggcacgc gcgagtttct taccgtcgag aaaggcgcgg tccacgtcga
      661 atcctccggg gacggcatgg atctggcggc cggcgattcg gccacctatg ccgccgacac
      721 cgcgcacgcg atcttgaaca cagggaaatc cgccgctgtt gtgttcctcg tggtgatcta
      781 cacccagccg cgctcccctg ccgcgcgctg aagggtttta tttggccgcc atgccggcca
      841 gcgccgcctt ccattccggc gagtccagca gagcctgggt ccaaatccgc gcgccctcct
      901 cgtccagatg atagccatcg ggaaagcgcg agggcggcag ggcaggggag gcatggagca
      961 cgcgggcacc ggcgttgacg gcggtttgtt tgaccaaggc agggagttca taggactctg
     1021 gcaggggcac cggcaggatc agcagggtgc cgccggatgc gccgatcgtg gtggcaaggc
     1081 tggcgaggtt tgagcaggag ggtgacgccc tggtggtcgg agccgaggcg ttgcgcgtct
     1141 tttgcaccaa attcactgtc tgctcgtagc cgggcatgaa attgtaaaaa accagcggct
     1201 gcacgcggcg gcgatgcgcg aacagggcgg aggcgcgcgc ggcggcggcc tggcaggtgt
     1261 cgccaaagcc gagtccctcg ctgcggacga aggcgggcag gtcagcccat gaggtgtaaa
     1321 aagcggccac ggaatccaga tcgcgcagtc cgtcctccaa atgcaggcgg ccagtgcaaa
     1381 ggaggacgag atcgggccgc gagcctgtct ggtcaaagta gcgccggtag ccatgccgcc
     1441 actcctcgac gcggctgccg tcgggatgca tcaccgagac agcgactgga cgccggctgg
     1501 cggcttcgag gccgcgcgcg agcagggcgc ggtccaatcc gcagcgcgcg agcgagttgc
     1561 ccagaatgag cactttcaac gtccccgccg gcgcctgccg gagacgcgcc gcctcggcgg
     1621 gaagcgagcg gatgtgggcc acatccttcg aaaggctcgt ctcgaacatg cgggcgccgc
     1681 actccagcag cagcagcgcg agcagcagcg cgaggacgac cagggactcg gtttttttca
     1741 tttcgtgaat cgccggagtg ttaaaaacgg aaatagatga actcggcggg cgcgggcggc
     1801 gggaagcaga tcatcatcag cacaagatag acataaagcc ccgcgcgcca ccaccaggcg
     1861 gacttgagca gggcctcgtc gtccttccgg cgctcaagcc aaaactcgta ggccagccag
     1921 ggcagcagga aaaaaaacgt catgcccgcg caggtgaggg tgaagtccgt gacctcccat
     1981 ttcccgccca gagcgccgag aagcgccagc gcgtgacgca ggctgtcggc gcggaaggca
     2041 agccaggtga ggcagacaag atggaaggtg acgaaccatt tggcgcctgc cagccaccag
     2101 ggagcggatg gccgcgtttc cgccgtgacg acgggctttt tcacccgcag ccagacgcgg
     2161 tgaagcccga gccaaagccc gtggatgcca ccccacacga tgaaggtcca ggccgcgcca
     2221 tgccagaggc cgccaagcag catggtgagc atcaggttgc gcgcggtgcc ccatccgccg
     2281 ccgcgatttc ccccgatcgg tatgtaaagg tagtcgcgca gccaggtgga gaggctgatg
     2341 tgccatctct gccagaagtc ccgcggatcg agggcgaaat aggggcggcg gaaattggtc
     2401 atcaggtcga agccgagcca gcaggccgtg ccgcaggcga tggcgctgta gccggcgaag
     2461 tcgccgtaga tttggaaggc aaaagcgtac acgcccagca gcacctccgg ccccgccaga
     2521 gatgacgggg acgccgcgaa cacatgcccg gccagccacg ccatgttgtc cgcgatgacg
     2581 actttcaaaa acagcccgct gagcaccaga taaaagccct tgcgaaaacg cgcctcgtcg
     2641 cacctcggcc gcggctgctc cacctgcgga agcagatgcg acgagcgctc gatggggccg
     2701 gcgacaagct ggggaaaaaa cgagatgtag agggcgaaat ccgagaagct gcggcagggt
     2761 ttggtctgag cccggtaaat atccatggtg taactcagcg cctggaaggt gaaaaaagaa
     2821 atgccggccg gcagcagcac ttcaagcgcc cagcgatggt cggcaaggcc cgcccacatc
     2881 agcagccggg acgcctcgcg tgtgaagaag ccaagatatt tgaacacacc cagcacggcc
     2941 aggctgagag tcaccccggc cgtcagccag cgccttctcg cccgcgggca ggcggcctca
     3001 tggatgcggc ggccggcgat gaagttcacc caggtgcaga agagaatgag cgaaagaaac
     3061 cgccagtcat agcaaccgta aaaaaaatag ctcgccagca gcagccagcg gttttgaccc
     3121 gcatgacgca tgagccggca tcccagggcg acgagggcga agaagatcca gaattcaaaa
     3181 ctgttgaacc acatgcgtga tggcggaccg ccgccgggcc ggtcctttca accctggccg
     3241 gggtggaatc aagcccgcag cctgcggccg gatgtctttt tgccgggcat gaaggtttct
     3301 tgcgggaagc ggcggcaggt gttatcgcca gcgcccaacg ggcgaggagc gctccggccc
     3361 aggccgtgtc gcaagttgcc cggtccggca tgtcatggag tttcgcttcg ccctgttctt
     3421 cctgtttctc tactacatcc gcccccagga ctgggtggcg ggatgggcgg gcttcaacat
     3481 catcaagccg ctcatggtgg tctggatcgc cgcgctctac accacgcgga accgccccgt
     3541 cgtgccgggc ctgctgcgga cgccgcacga ctggatggtc ctcctctact atgcgtatgt
     3601 tgtctggaac gcgcccgaca ccaaagccgc cttcatgggg ttcatgccgc tggccgtctt
     3661 ctatgcgctg acggttcagt ccctcgtcag ttgggaaagg gtgctgggct acctgaaggc
     3721 atggaatttc atgctgcttg gcgtggccgc gatggcggtg ggcagtcttt acgggctgga
     3781 tctcaccggc gcagtggatg tgaccgccag aaatgccgga cgcctctcga tcggcacctg
     3841 gctgcacaac aaccccaact ccctggcgca cactgtcatc gtggccatcc cgctgagtta
     3901 cctgctctat ttctacaaag gctcggccat gggccgcctc gtcatctttc ccctttgcgc
     3961 cgcgcttgcc gccgagtgcg cgtttcacgc ctcctccaag gggtccttcc tcgtcgccgg
     4021 cgggctcgtg gtcttgatct ttgtcattgg caggccgctg gggataaaag ttctcgccct
     4081 gtccgccgcc gccacgatgg gcgtctcggc cttgagcttc cttccacgca tgtccggcat
     4141 gtccagcctc agcagcaatg agggcgtgca gggcaggctc atggcctggg agatggcgaa
     4201 gaccgttctc gactccagca acacgggtgt cggctggaag cagttcgtcg cctgggtgtc
     4261 ttgggagggt gagaccttcg tcaaagccac ccactccagc tatgtgcaga tcggggcgga
     4321 cctgggcgtt tacgggctgt ttatttacct gaccgccctt tggaccgccc agcgcagcct
     4381 tttggcaacc catcgcttta cccggcatga ccgcacccgc gagcaatgca ggcgtgcggg
     4441 catcgtcctc gtcagcgcct acgcggtgtc gaactggatg atcaaccgcg aatatcactc
     4501 ggagtacttc ttgatgatcg ccgtggccgc ggccatccac cgcctctgca tttatgaatc
     4561 caggcaggaa agccagccag agccatcccg ggtggctgcc atgccggtca atcgcaggtc
     4621 aaatcctgcg cggaagcagc acgtctcccg tcaagaggag gagccttcct gggaagactc
     4681 tccgccatct ctcaagtctg gtccggactg gaaggacgcg gtcgctggag tggcgctgac
     4741 ctggtgtgtg gtggaaatct gggactatgt gctgacgagc ctgtgatcag ggatcaagga
     4801 tccgtcaggt tcggcgccca gagcgggcct tccttattca cgggaggcgt gacgccttcg
     4861 gcgggaaagg tgcccttggc cgcggcgcgg actttttcac tcagctcggc caccgtggcg
     4921 gcgtgcgcgg ggtcggcggc caggtttttc agctcgccgg ggtcggcgtc atgatcataa
     4981 agctcgctgc cctccctgcc ctcgtcccac tccgtgtagc gccagcgcgg cgtgcgcagg
     5041 ctgtagccga agaagcgccg tccctccgag cctttgtcgc gtgaaaccgt ggcacggttc
     5101 ggcccgccgc cgcgcgagac ctgggtgatg gcccaaccgc ggcctttttc ctgcgggtct
     5161 ttgagaatgg gcacgaggct ctggccctgg agattcgcgg gcgccttgac accgcacagc
     5221 tcggccagcg tggggtagag atcgagatga ctcacgggcg tggcggccac gctgccgggt
     5281 ttcgtgactc cgggggcgac gatgagcagg ggcacacgcg cgctgccctc gaagaggctc
     5341 atcttttgat aaagcccgtg ttcgcccatg tggtagccgt ggtcgctggt gaaaacgatg
     5401 accgtgctct cggcgaggcc gtttttgtcc agcgcctcca ccacgcggcc cacctgcgcg
     5461 tccatgaagc tgatgctggc atagtagctt tgcgcacacc tgcgtcgcag ttcgtcagtg
     5521 agcttgtcct gctctttctt gtagctgccg agcgcggcgg cgggcacctc gggcgggttc
     5581 tggtccacgg tggggtggac gggcatttcc gcctcgggat accagccgaa atagggcgtc
     5641 tccggcgcca catagggcgt gtgcgggcgg aaaaagccga cggcgaggaa gaaggggcgg
     5701 tccttctttt tcgcgcagcg ctccagcacc cactcggcct cgctggcgat catgccatcc
     5761 gtgtgatgcg cgtcgctctt tggcgaggca taccagctca gcgtgccgcc gaactggccg
     5821 ggcgtgaggg tgaaaatctc cggctcctcc tccatgcggt ccaccccggc ggggttgatc
     5881 tccatctccc aggagccggg gtcgtcgtgc ccgtccgtgc ccacggactt cggcacattg
     5941 taatggtaga gcttgccgat gcgcgcggcg aagtagccat tctgccggaa tgcctgcggc
     6001 aggctgatgt gggaggggat ggactggcgg aagatctgcg agttggtgag gatgccgttg
     6061 ctgttcgggt agaggccggt gagcatggag ttccggctcg gtccgcagag ggggaaggtg
     6121 cagtaggagc gctcaaagcg caccccgcgc gcggccagct tgtcaatgtt cggcgtcttc
     6181 gcgcgcgggt ccccgtagca gccgaggtag ttgttcaggt cgtccgagat gatgaagagc
     6241 acattcttgc gctcctgcgc ggcggcgggg aaaacggccg cgcacagcag cgcggcgaga
     6301 cagaggcggg agatcaggcg catggttcgg gtgagttgga accgtccaca acgaaacgcg
     6361 gcggggggac tttcggtgaa gatgaaacgg cccggcttca caaggcggcg aatggcacag
     6421 gtcacggcct gcaaaaaagt catcttcctc tcaaagattg acgatgatcg cggcggcgaa
     6481 tacaaccgct gcgaaattcc ttccaaatta tgtccaaaaa gctcaagccg gggttgttca
     6541 acgcgagttc gccatccatc aagttcaccg atgttctcaa ggcgatcgaa aacaaaggat
     6601 acgcgctgcg cgcacgcgga gaatttgacc tgaacgtggt cggtgtgcgc agaacgaaga
     6661 tcaaggtgga cgccttcaac gatgtgctgt tcgccttttt cccctatcaa ggccagtggc
     6721 agtatcagaa gtaccagatc accaccctgc ctggctacac ttacctggcc gaaaggctgg
     6781 gcaacagcaa aggcaccgcc attctcgtgc cgggccatta caaggacaaa tacgaggtcc
     6841 gccagcatgg cagcggcggc tttgcccacc aggctctctg ccagaaaaga acgtccggcg
     6901 gttatgtcag cggcatcaaa gtctggcgcg acgacaccct cgacgccgtg ccgaatcccg
     6961 acaccagcaa gacgtatgaa ggcggcggca tcaacatcca ccgcgcctca gcgagcgact
     7021 gcacggtgcg cgtggccgac tattccgcag gatgccaggt cttccgctgc gtggccgaat
     7081 tcgacgcttt catgaccacc acgcgccgcg ccagggacgc ctggggcaat accttcagct
     7141 acacgctgct cgatgaggcg gacctgtgat tcccaacaac aaacatccat ccaccatgtc
     7201 caagaaagcg tccaaaaaaa cagcggccaa aaaggccgca gcacctgcgg cgacaccctc
     7261 ggtttcactc ccgcagcttg tcgtcaccgc aagttcgttg aatgtgcgcc gctctccgtc
     7321 attgacggca actgtcactg gcagcctttc cagcgggacg gtcgtggact ggcttgaaac
     7381 ctcccctgac gagctttggg ccaaagtccg gcatgggact ttgagcggat ggtcgagccg
     7441 gcgttacctt gccgaaaccc aggcccccgc gccaggcggg ccttatgatg agattcttcg
     7501 catcgccgac acctcggcca tcgcgcaata tcggtggctg gaccgtggac gcgcgccaag
     7561 ggcctacatt cgcggcatgg cgctggcctt tgccagggtc tattgcaagc tcagcgcagg
     7621 cgatcctgcc gcgatggaaa tggcgaaagc caacacggga gtggcagacg tggacgccct
     7681 ggctcattac gcgcagattt tccacgaggc gggaatgaac aacgagtctt cgggcgcctc
     7741 caccctcagg catttgtatg ttcttctcgt tggcctcggc atgagggaaa gctccgggaa
     7801 acactgcgag ggcagggaca cgacggcggg caatgtcacg gccgagacag ccgaggcggg
     7861 gctttttcaa acaagctgga atgctcgaat tcgtccagac atgacgacga tccaaccaat
     7921 ggtccaagtg ttcaatcact accgtgcaaa tccgcagggc ttcctggaaa tttttcagga
     7981 gggcgtgaca tgcagtccgc agaactggca taaccatggc accggccttg gcaggacttt
     8041 ccagcagctt tccaaagagt gtcccgcttt tgccgccgaa tttgcagccg tcggcctgag
     8101 aaaccgccgg cgccactggg ggccggtcaa ccggcgcgag gctgaggtgc gccctgagtg
     8161 caacaccatg ctgctccgcg tccagcaggc ggtggacagc ctccagctat gcccggtgtg
     8221 acaggcttgt cttcatgaac tcactcacac atttaacagc cgccttcgct gcgctgctcc
     8281 tcctggcctc atgccagacc cagcccggtg tttcaggaac tgcggcgctt caacccggcg
     8341 acatcgtttt ccaggattcg tcccccgtct caggccaggc ggaggcgatc aagaaactca
     8401 cgcgctcgga ctggagtcat tgcggcatct acttcaagga ctccagcgga aagggcgtgg
     8461 tggtggatgg caacggtttg tcgaaagccg tggcatggga aaaatggcgc gccaacggca
     8521 acggcggacg ttatgccgcc tggcgcgcgg tgaaccctct gacggcggcg cagatcagcg
     8581 ccttgcgcaa agccgccgac catctggatg gccgcattta cgacaagcgc ttcgcctggg
     8641 acgacgagcg catctactgc tccgagatca tttggaaagc ctggcgcgcc gcctttcagg
     8701 aggaactctg ccctttgcaa aagctcggcg acttcgatct cgccagccgg gaagcgcgcg
     8761 cgctcattga gcgcccggac agttggggca gcctggagaa tgccctccgt caccgggacg
     8821 aacccgtggt gtcgccgcag aagctgacgg agagcgcgct gctgcgccgt attcgctgac
     8881 cttatgcgcc gctttatctc cagtcttttt gttttcgggt ttgcatctcc cgtctttccc
     8941 atgggtccgg ggactggagg ggcggatcat tttctcgcca aagattcagg ggcatccgcc
     9001 tggtcggtcg cagtcactca aaaaggaaac ctgccggatg aaaaactgcg cggcctcgtg
     9061 tggcgcgcac ccggacgtcc acagggtgtc atcgtctgcc tgcacggctt gcaaacacac
     9121 tcggcatggt tcgcgccggt ggctgcggat ttgcggaacg ccggctggac tgtcatcgcc
     9181 ccggaccgcc gggacagcgg catgaaccgt cagcaaaata tggctcccgt gaaaagcgcg
     9241 gagcagttgt tcggcgacct ctccgcccac ctggagcaag ccgccgccga agctccggga
     9301 aagccgctca tcctgctcgg cacctcctgg ggcagcaatc tggccaccgc ctacctggtc
     9361 cggcgccagg acgcgcgggt gagcggactc atccagctcg tgcccgccac acaggtgcgc
     9421 aaaaacctcg cgccgaaatt cgcggagcgc gtccttggcc cggttctgag cgtgctctcc
     9481 ccgcgcatgc cggcacgccc cacattctgc gacttccact acctcgccgg cggccgaagc
     9541 cagtcctccg gcaaggggcc tcctgccatt gtggaccgcc gggcgccgct ccccgagccg
     9601 cctgctctcg aagactccaa ttcaaaactg gcctgcattt tgaaaaagga tgactccctc
     9661 ctgcgcgagc cctccatccg caccctgcgc gtgggactcg tccttgcgga gatgtggaga
     9721 aaaaaaacct ccgccattcc tgataacaca ccgatcctca ttctcaccgc cggccatgac
     9781 cagatcatgg acaaccgcgg cgccctcgcc gcctttcccc aaggacgcgt cactcatcgt
     9841 gacatgcctg cgggacatgg cgtgcagatc actcacccgc attgggtgag ccgtcacgtc
     9901 ctcgaatggc tgcgctccgc cgggaagcgc ccctgacaac cccgcccgcg caagaagccc
     9961 cgcccgcgcc tcgtagcagg ggtcatgcgc tttcgctcca tcctggccct gcccttcgcc
    10021 gccgccgtgc cggtgctggc ggagccgtcg gcgcgggatg tggagttttt tgagaaaaaa
    10081 gtgcggccca tcctggtcga gcgctgctac gagtgccact ccgccgagag cggcaagagc
    10141 aagggcggcc tcacgctcga ctcgcagccc gccatcctcc agggcggcga caacggcccc
    10201 gccctcgtcg ccggggatcc cggcaagagc ctgctcatcg aagccgtgcg ctatcaaaag
    10261 cgcgagctgc aaatgccgcc caagagcccc ctgcccgccg ccgagctgcg cgtgctagag
    10321 gagtgggtgg cgcgcggcgc gcccgatccc cgcaccgagc cggtggcctc c
//
LOCUS       DAOMYJ010000188        10354 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Prosthecobacter sp. isolate AMR_MDS_5629
            NODE_2148_length_10354_cov_21.622002, whole genome shotgun
            sequence.
ACCESSION   DAOMYJ010000188 DAOMYJ010000000
VERSION     DAOMYJ010000188.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867850
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prosthecobacter sp. (wastewater metagenome)
  ORGANISM  Prosthecobacter sp.
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Verrucomicrobiaceae; Prosthecobacter.
REFERENCE   1  (bases 1 to 10354)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10354)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.30
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:34:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,742
            CDSs (total)                      :: 4,684
            Genes (coding)                    :: 4,676
            CDSs (with protein)               :: 4,676
            Genes (RNA)                       :: 58
            tRNAs                             :: 55
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10354
                     /organism="Prosthecobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2148_length_10354_cov_21.622002"
                     /isolate="AMR_MDS_5629"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1965333"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1234
                     /locus_tag="PLP58_16720"
     CDS             <1..1234
                     /locus_tag="PLP58_16720"
                     /inference="COORDINATES: protein motif:HMM:NF041518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="choice-of-anchor Q domain-containing protein"
                     /protein_id="HRJ10223.1"
                     /translation="TGGTTVDASSLPAGLAVSGGGASRIFNVASGGNLTLRSLTLTGG
                     NADGGGAITNDGMLLLDRCALTGNASTTNGGALRNNNLLTATRCTFSGNTAVTNGGAV
                     TGNSGTAEFISCTFSGNATTGPTQGGGALNHASGAIAMTLTHCTIAGNSSAGSEGGGG
                     IRNRGTGLTVENSIVAGNTITAGTGADFNSTETLTRSGANVIGSRATVLAPFPAGAPN
                     ANGDYVGTAGAPMPALLAPLASYGGPTQTLALLPGSPARNAATGTATSDQRGFPIIGT
                     PDIGAYEAGNNLATIYNAWIWETLPAIATTPQHATTFDFDGDGQTNEAEWLALTDAAN
                     PNSRFAATPARNGANLEITVPTAAGRIYTLLQSGTLVGGSWSAAPGFTPQPGTGIPII
                     FQVPIASTRMFYSVQVSP"
     gene            1353..1994
                     /locus_tag="PLP58_16725"
     CDS             1353..1994
                     /locus_tag="PLP58_16725"
                     /inference="COORDINATES: protein motif:HMM:NF013921.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - transposition, DNA-mediated
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HRJ10224.1"
                     /translation="MPFQPFSDRKPVRIYYTNMPHWRQEGCTYFVTYRLADSIPENVL
                     HQWEREKLHWLSLRGIHVEKWPEGFARLDPAAQHAFLKHFNRALNRCLDDGHGSCALR
                     DTECQRIIADGWRHFAGMRYELGDLVVMPNHVHLLLTPLPGHELEDLMGSRKRQSARE
                     INLRLNREGDFWQKHSYDHIVRDEIELRAFQKYIAENTAKARLRQGEYLHLVP"
     gene            2203..3087
                     /locus_tag="PLP58_16730"
     CDS             2203..3087
                     /locus_tag="PLP58_16730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009960533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HRJ10225.1"
                     /translation="MPQLAAFPKAFMVALCKEGTMTVSEWIKLASTLDVQGLEWYAGF
                     LEMADEKNWPRFRKQVEDTGKVIPMMCCSPDFTHPDAAFREKEIAKQKRWIDMTHTLG
                     SSYCRVLSGQRRPELSIDEGVKLAADSIHACLPYAQERGITLIIENHYKDDFWEYPEF
                     AQKMDVFCKLVDAVNHPNFGVNYDPSNTYLAGEDPIELLKRVSHRVVTMHASDRYLAE
                     GTIEDLRKEEGGAQGYAKRLRHGEIGKGLNDYDAIFTELKSKGFNGWISIEDGVDGME
                     QLARSVEFLKKKIAHHWP"
     gene            3130..3690
                     /locus_tag="PLP58_16735"
     CDS             3130..3690
                     /locus_tag="PLP58_16735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007413279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF-type sigma factor"
                     /protein_id="HRJ10226.1"
                     /translation="MKNDVTPTTIQEPVSSSRLLPLVYEELRRLARGQMAGESGAQTI
                     SATVLVHEAWLRVSRDKEQRWESRRHFLGAAAQAMRCVLINRARDKKRLKRGGEDAER
                     VDLDALGEAEAALAFDAPDEELLAVDEALDRLTAEDALAGEIVRLRYFAGLTWPEIAE
                     VTGMSERDLNRQWAFARAWLRDAMEK"
     gene            4368..4862
                     /locus_tag="PLP58_16740"
     CDS             4368..4862
                     /locus_tag="PLP58_16740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ10227.1"
                     /translation="MKTALLILTSVFAVALPASAQFKDTELKVSPRTLDREKQINRPK
                     QQELTRGLKITVKNTSTQPLGEGEIEWALLVQRPSLQRKALLSTGKEKLPPLKASEVV
                     TFNVGAIPVQDTGKTRQEMEYQVIVHRGGIEVAKAESTPKFSQMAQSARGANKKGAGN
                     GKAK"
     gene            4919..7816
                     /locus_tag="PLP58_16745"
     CDS             4919..7816
                     /locus_tag="PLP58_16745"
                     /inference="COORDINATES: protein motif:HMM:NF025253.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0098024 - virus tail, fiber [Evidence
                     IEA];
                     GO_process: GO:0019062 - virion attachment to host cell
                     [Evidence IEA];
                     GO_process: GO:0046718 - viral entry into host cell
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber domain-containing protein"
                     /protein_id="HRJ10228.1"
                     /translation="MKNTHHLPLPLCLLILVILHSSLGLLHSQVPQLVSYQGRVAIGT
                     VNFEGTGQFRFALVNAAGTTVYWGNAADTTPADGIPDSAVSLAVTKGLYSVLLGDTSL
                     TNMAAIPASVWTNADVRLRVWFNDGVNGNQLLTPDQRLAPYGYLPDGSISTVKIATGA
                     VDSSLLAANAVTGPKIASNAVNSAKLASNLTLGGTTTGTFSGNLTGNASTATNATSFT
                     GALAGDVTGTQSATSIAAATVTGKALTGFSSTTGTITASDTILSAINKLNGNVALKAP
                     IASPTFTGTVSGTFSGNGAALTNLNGANLTAGSIGGSQLAAASVTAAKLGTDVGLWSV
                     SGANVFRSAGNVGIGTSSPGRALQVDGTSPAISIGSEFNGRTSLLMAVTAISGGYSII
                     QSTSSAGGSYGALSLQPNGGDVGIGTETPTAKLDVNGGINFSGIISGNGSALTNLNGG
                     NLTAGTVTAAKLGTDVGTWAASGGNAYRTTGNVGIGTTTPSQPLTVAGNTHLGTGDNA
                     ALAFDGLGYGRLGFVKKSGANPVIAAGFEQPIVFSQSNQASVLTNVAGSTFTERMRID
                     YDGNVGIGATVPKGLLHVAGDYYGKGHLTLYAYEGDGVSGTAYIQGRDDSVGSSIGLQ
                     LRTQNAGAEVDALKIAPNGNVGIGTANPTHILDVNGVSQSQGLELRRVGIPPYLDFSS
                     NLVDDFNARIIWQGAPVNRLDFDAPKFSFLNGTTSLHILPGEINGVASAGDVTLEIPG
                     GHTLGIWDSLSVNNNLTVVGNSNMVGYAAVGVGGVPPNAALAVKKSASSHVTAAFLYE
                     TGTAVASIGSPTDALYVNVATGYARRSDDSPFWSVPSDRRLKTGIVQVTDALDTVSRL
                     NPVRYHYTAERLAKMPGSVDKEHYGVIAQEYQKLFPQFVTTGADGYLSVQSDPLIFVA
                     TAAIKELNAKHDSAIAAKDAEIAALKKQLSSIDARLARLEATAPVKVAVKK"
     gene            7942..8805
                     /locus_tag="PLP58_16750"
     CDS             7942..8805
                     /locus_tag="PLP58_16750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ10229.1"
                     /translation="MLLAATANAGPRTSASYTITADTVSHGGTRTTSAAYTNDGTAGG
                     IVGISIVTTPAETAKHGYIGQLTEVTALQLAASPTTVNEGTTRQLTATATLDDATTSA
                     LLATDVSWSVQSGPLTSISTSGLATAGIVYQNTAATAQGLYSGITGTLNLTVINTNTD
                     DIPGYSGDGLDDAWQVQYFGLNNPLAAPLLDPDGDGHTNVFENLAGIDPTSAASFFRV
                     TQISISGGVFNMTFPTVPGRSYQLQSSSNLANPWINIGAATAGTGGMVTLPVDVTGQT
                     KHFFRVSVGAP"
     gene            9252..9968
                     /locus_tag="PLP58_16755"
     CDS             9252..9968
                     /locus_tag="PLP58_16755"
                     /inference="COORDINATES: protein motif:HMM:NF013921.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - transposition, DNA-mediated
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HRJ10230.1"
                     /translation="MAWLIPAHIPLPEDFVGFDPESSLTKDFEAYERHLPHWRVPGCC
                     YVLTFRLADSIPVAVMVEMQAEAEAWQKRLAEAAQRHGGKLPPEELAAWQEFQQVRMR
                     KLESLLDEGRGECLLRHPEHQQPLVEALHHFEGSRCEMLAYAIMPNHVHVLCRPLGEH
                     ALESLTRSWKRHSSDRIHRRLGRSGSLWQEESFDRIIRDAAHYQQAVRYIAKNPLRAG
                     LVPPEAVVWLHPRIVAANAG"
BASE COUNT         2078 a         3417 c         3106 g         1753 t
ORIGIN      
        1 caccggcggc acgaccgtgg atgccagcag cctgcccgcc gggctggcgg tctccggcgg
       61 cggcgcgagc cggattttca atgtcgcatc cggcggcaat ctcacactcc ggagtctgac
      121 gctcaccggc gggaacgccg acggcggggg cgcgatcacc aatgatggca tgctgctgct
      181 cgaccggtgc gcgctcaccg gcaatgcatc cacgaccaat ggcggcgcgc tgcgcaacaa
      241 taaccttctc acggcgaccc gctgcacctt cagcggcaac accgccgtga ccaacggcgg
      301 cgcggtgacc ggcaacagcg gaacagccga gttcatcagc tgcacttttt ccggcaatgc
      361 cacgaccggc cccacccagg gcggcggcgc gctcaaccac gcctcgggtg ccatcgccat
      421 gacgctgacc cactgcacca tcgcggggaa ctcctccgca ggcagcgaag gcggcggcgg
      481 catccgcaac cgcggcacgg gcctcacggt ggaaaacagc atcgtcgcag gaaacaccat
      541 caccgccggc accggcgcgg actttaacag cacggaaacg ctcacccgca gcggggccaa
      601 cgtcatcggc agcagggcca ccgtcctggc gccgtttccg gccggcgcgc cgaacgccaa
      661 cggcgattac gtcggcaccg ccggagcgcc catgcccgcg ctcctcgccc cgctcgccag
      721 ctacggcggc cccacgcaga cgctggcgct tctccccggc agccccgcgc gcaatgccgc
      781 caccggcacc gcgacgagcg accagcgcgg cttccccatc atcggcacgc cggacatcgg
      841 cgcgtatgag gcggggaaca atctcgccac gatttacaac gcatggattt gggaaaccct
      901 ccccgccatc gccaccacgc cgcaacacgc caccactttc gatttcgacg gcgacggcca
      961 gaccaacgaa gccgaatggc tcgccctcac cgacgccgcc aatccgaaca gccgtttcgc
     1021 cgccacgcct gcgcgcaacg gggccaatct cgaaatcacc gtccccaccg ccgccggccg
     1081 catctacacc ctgcttcaga gcggcacgct cgtcggcgga agctggagcg ccgcccccgg
     1141 cttcaccccg caacccggca ccggcatccc gatcatcttc caggtcccga tcgccagcac
     1201 acggatgttt tacagcgtgc aggtgagccc gtagcttcgg ttggcaaccg aagccgagcg
     1261 tccaccggtg tgctttccac cttgagggat tcggttgcca accgaatcta cccacattcg
     1321 gctgccaccg aatctacacc gcacctgctc tcatgccctt ccagccgttc agtgaccgca
     1381 agccggtgcg gatctactac accaacatgc cgcactggcg gcaggagggc tgcacttact
     1441 ttgtcactta ccgcctcgcg gactccatcc cggaaaacgt gctgcaccaa tgggagcgcg
     1501 agaagctcca ctggctgtcc ctgcgcggca tccatgtcga aaaatggccc gagggcttcg
     1561 cccggctcga tcccgccgcg cagcacgcct ttctcaaaca cttcaaccgc gccctcaacc
     1621 gctgcctcga cgacggtcac ggctcctgcg ccctgcggga cacggaatgc cagcgcatca
     1681 tcgccgacgg ctggcggcac tttgccggca tgcgctatga actgggcgat ctcgtggtga
     1741 tgcccaatca cgtccacctg ctgctcaccc cgctgcccgg tcacgaactc gaagacctca
     1801 tgggcagccg caagcgccag tccgcccgcg agatcaacct gcgcctgaac cgcgagggcg
     1861 acttctggca aaaacacagc tacgaccaca tcgtccgcga tgagatcgaa ctgcgcgcct
     1921 ttcagaaata catcgccgaa aacaccgcca aggcgcggtt gcgccaaggc gagtatctgc
     1981 atttggtccc gtagcttcgg ctggcagccg atgacctgta gcttcggttg gcaaccgaag
     2041 ccgagcgtct ccaggcatgc ccccacccca gaatgattcg gctgccagtc gaatctacgt
     2101 ccattcggct gccagccgaa tctacgcagc gtcgcaaatc acacacactt tgtcgtggca
     2161 tcggcgatag cctctggctt gatcattcgt catttcatcc acatgcccca actcgccgcc
     2221 ttcccgaaag ccttcatggt cgccctttgc aaagagggga cgatgaccgt ctccgaatgg
     2281 atcaagctcg cctccacgct cgatgtgcag ggcctggagt ggtatgcggg cttcctcgag
     2341 atggccgacg agaaaaactg gccgcgcttt cgcaagcagg tcgaggacac cggcaaggtg
     2401 atcccgatga tgtgctgctc gcccgacttc acgcatcccg acgccgcgtt tcgcgagaag
     2461 gagatcgcga agcagaagcg ctggatcgac atgacgcaca cgcttggcag ttcgtattgc
     2521 cgcgtgctca gcggccagcg ccggcccgag ctcagcattg atgaaggcgt gaagctcgcc
     2581 gccgattcca tccacgcctg cttgccgtat gcgcaggagc gcggcatcac actcatcatc
     2641 gaaaaccact acaaggacga cttctgggag tatcccgagt tcgcgcagaa gatggatgtc
     2701 ttctgcaagc tcgtcgatgc cgtgaaccac ccgaacttcg gcgtgaacta cgacccctcg
     2761 aacacctacc tcgccggtga ggaccccatc gagctgctga agcgcgtctc acaccgcgtc
     2821 gtcaccatgc acgccagcga ccgctacctc gccgagggca ccatcgaaga cctccgcaaa
     2881 gaagaaggcg gtgcccaagg ctacgccaaa cgcctccgcc acggcgagat cggcaaaggc
     2941 ctcaacgact atgatgccat cttcaccgag ctgaagagca aaggcttcaa cggctggatc
     3001 agcatcgagg acggcgtgga cggcatggag cagctcgcgc ggagcgtcga gttcctgaaa
     3061 aagaagatcg cgcatcactg gccatgagtt gcttgcagtg cagggatcga aggacatggt
     3121 gatcagccca tgaaaaacga cgtgaccccc acaacgatcc aagagcctgt ttcatcgtcg
     3181 cggctgcttc cgctggtcta tgaggagctg cggcggctgg cgcgtgggca gatggcgggg
     3241 gagtccgggg cgcagacgat ctcggcgacg gtgctggtgc atgaggcgtg gttgagggtc
     3301 tcgcgggaca aggaacagcg ctgggagagt cgtcggcatt ttttgggcgc tgcggcgcag
     3361 gcgatgcgtt gcgttttgat caaccgcgcc cgtgacaaga agcgcctgaa gcgcggtggt
     3421 gaggatgcgg agcgggtgga cctggatgcg ctgggtgaag cggaggccgc gctggcgttt
     3481 gatgcgccgg atgaggagct gctggcggtg gatgaggcgc tggaccggct gacggcggag
     3541 gatgcgctgg cgggtgaaat tgtgcggctg cgctacttcg cggggctcac ctggccggag
     3601 atcgcggagg tgacgggcat gtcggagcgg gatttgaacc gccaatgggc ttttgcgaga
     3661 gcgtggctgc gggatgcgat ggagaagtag agatcgtagg ccggatgtgt tcggtgaggt
     3721 gagcaccgct aacacggagg atctgcttca ccgaactatc cggccggttt ggaggtgtgc
     3781 gggggtgaaa gagcattcgt gcgctcaaag agccggatag tttggcgaat tgggctgggg
     3841 ggtgaaacga cacgctttcc gccaaacaca tccggcctac gacacggcgg taggccggat
     3901 gtgttcggtg aggtgagcat cgctaacacg gaggagctgc ttcaccgaac tatccggctg
     3961 gtttggaggt gtgcggaggt gaaagagcct tcgtgcgctc aaagaagccg gatagtttgg
     4021 cgaagtgggc tgggggtgaa atgacgcgtt ctccgccaaa cacatccggc ctacgacatg
     4081 gcggtaggcc ggatgtgttc ggtgccatga gcatcgccaa cacgaaggag ctgcctcacc
     4141 gaactatccg gctggtttgg tggtgtgcgg aggtgaggcg cattcgtgcg ctcaaagaag
     4201 ccggatagtt tggcgaagtg cgctgggggg tgaaatgacg cgcttttcgc caaacacatc
     4261 cggcctacga cacggcggct ggtgcttccc ggcaaaaaag tttcttcttt tttgccgggt
     4321 tcattctctg acggggcatt gcctagtgga aacccatttt ccacgtcatg aaaaccgcac
     4381 tcctcatcct cacctccgtc tttgcggtcg ctttgcccgc ttccgcccag ttcaaggaca
     4441 ccgaactcaa agtcagcccc cgcacgctcg atcgggaaaa gcaaatcaac cgccccaagc
     4501 agcaggagct gacacgcggc ctgaaaatca cggtgaaaaa cacctccacc cagccgctcg
     4561 gcgagggcga gatcgagtgg gccctcctcg tgcagcggcc cagtctccag cgcaaggcgc
     4621 tgttgagcac cggaaaagag aagctgccgc cgctgaaggc ctccgaggtg gtgactttca
     4681 atgtgggtgc catccccgtg caagacaccg gcaagacccg gcaggaaatg gaataccagg
     4741 tcatcgtgca tcgtggcggc atcgaggtgg cgaaggcgga gagcacgccc aagttttccc
     4801 aaatggctca atcagcccgt ggggccaaca agaagggcgc cgggaacggg aaggcgaaat
     4861 agcccgcatg agcccttcat ccgccctttt gcctcctcac gccttaccca ctcacgccat
     4921 gaaaaacaca caccaccttc cgctgccact gtgcctttta attctcgtca ttttgcattc
     4981 gtcactcggc cttttgcatt cgcaggtgcc gcagctcgtc agctaccagg gccgcgtcgc
     5041 catcggaacc gtgaatttcg agggcaccgg ccagtttcgt ttcgccctcg tgaatgcggc
     5101 tggcaccacc gtctattggg gcaacgccgc cgacaccacg cccgctgatg gcattccaga
     5161 ctccgccgtc tccctcgcgg tgacgaaggg cctctactcc gtgctcctcg gcgacacctc
     5221 gctcacgaac atggccgcca tccccgccag cgtgtggacg aatgccgatg tgcgcctccg
     5281 cgtgtggttc aacgacggcg tgaatggcaa ccagctcctc acgcccgacc agcggctcgc
     5341 gccttacggc tacctgccgg atggcagcat ctccacggtg aaaatcgcca ccggtgccgt
     5401 ggatagctcg ctgctcgctg ccaacgccgt caccggcccg aaaatcgcca gcaatgccgt
     5461 caacagcgcg aagctcgcca gcaacctcac gctcggcggc accaccacgg gcacttttag
     5521 tggaaacctc acgggtaacg cctccacggc caccaacgcg acgagcttca ccggcgcgct
     5581 cgccggtgat gtcactggca cgcagagcgc cacctccatc gccgccgcca ccgtcaccgg
     5641 caaggcactg accggattct cctccaccac cggcacgatc actgccagcg acacgatcct
     5701 ctcggcgatc aacaagctga acggcaatgt cgcgcttaaa gctcccatcg cctccccgac
     5761 cttcaccggc acggtgagcg gcaccttcag cggcaacggc gcggcgctta ccaacctcaa
     5821 cggcgcaaac ctcacggcgg gcagcatcgg cggttcccag ctcgccgccg catccgtcac
     5881 cgccgccaag ctcgggacgg acgtgggcct ctggagtgtc agcggcgcga atgtcttccg
     5941 cagcgccggc aacgtcggca tcggcaccag ttcaccgggg cgggcacttc aggttgatgg
     6001 aacctccccg gcgatttcca ttggcagcga attcaatggc aggaccagcc tgctgatggc
     6061 agtgacggcg atttcaggag gttactccat cattcagagc accagcagcg ctggtggctc
     6121 ctatggagcg ctcagcctgc agccgaatgg aggcgatgtc ggcatcggca ctgaaacacc
     6181 cacggcgaag cttgatgtga acggcggcat caactttagc gggatcatca gcggcaacgg
     6241 ctctgcgctc acgaacctca acggtggcaa tctcaccgca ggcacagtga cggcggcgaa
     6301 gctcggcacc gacgtaggca cctgggcggc gagcggcggc aatgcctacc gcaccaccgg
     6361 taacgtcggc atcggcacca ccacacccag tcagcctctc accgtcgccg ggaacaccca
     6421 cctcggcacc ggggacaacg ccgccctcgc cttcgacggc cttggctatg gccgcctcgg
     6481 cttcgtgaaa aaatccggtg ccaatcccgt catcgccgct ggatttgaac agcctatcgt
     6541 cttttcccaa agcaaccagg ccagcgtgct caccaacgtc gccggctcca ccttcacgga
     6601 gaggatgcgg attgattatg acgggaacgt cggcatcggc gccaccgtgc caaaaggtct
     6661 cctgcatgtg gctggcgact attacggcaa aggtcacctg acgctctatg catacgaagg
     6721 ggacggggta agcggcaccg cctacatcca gggccgcgac gacagcgttg gcagcagcat
     6781 cggactccag ctccgcacgc agaacgctgg cgctgaagtg gatgcgctga aaatcgcgcc
     6841 caacggcaac gtcggcatcg gcacggccaa ccccactcat attttggatg tgaatggagt
     6901 cagtcaatcg caggggttgg agctgcgacg ggtgggaatc ccgccgtatc ttgattttag
     6961 cagcaacttg gttgacgatt tcaatgcccg catcatttgg cagggagcac ccgtcaatcg
     7021 cttagatttc gacgccccta aatttagctt tctgaatggc accaccagcc tgcatatcct
     7081 ccccggtgaa atcaacggcg tcgcctccgc cggggatgtg acgctcgaaa tacccggagg
     7141 gcacaccctc ggcatctggg acagtctttc cgtgaacaac aacctcaccg tggttgggaa
     7201 cagcaacatg gtaggctacg cagccgtcgg ggtcggaggc gttcccccca acgccgccct
     7261 cgcggtaaag aagtcggcaa gctcccacgt tactgcggct ttcctctacg aaaccggaac
     7321 tgcggttgcg tccatcggat cccccaccga tgcgctttat gtgaatgttg ccaccggcta
     7381 cgcccggcgt agcgatgaca gccccttctg gtccgtcccc tccgaccgcc ggctcaagac
     7441 tggcatcgtc caggtgacgg atgcgctcga caccgtcagc aggctgaacc ccgtgcgcta
     7501 ccattacacc gctgagcgcc tcgcgaagat gcccggcagc gtggacaagg agcactacgg
     7561 cgtcatcgcc caggaatacc aaaagctctt tccccaattc gtcaccaccg gggccgacgg
     7621 ctacctcagc gtgcaatccg acccgctcat attcgtcgcc accgccgcca tcaaggaact
     7681 caacgccaag cacgacagcg ccatcgccgc caaagacgcc gagatcgccg cgctgaaaaa
     7741 gcagctcagc agcatcgacg cccgcctcgc ccgcctcgaa gccaccgcgc cagtgaaggt
     7801 cgcggtgaaa aagtaacgcg atcaccgaag accatgaact caacgaacgt gaacaaagtg
     7861 gtccgcacag tcccctgtgc ggaaagagcg tccaccggac ttcgaatccc gcacagagga
     7921 ctgtgcggac cactctgtgc cttgctcctc gccgccacgg caaacgccgg cccgcgcact
     7981 tccgctagct acaccatcac cgctgacacc gtcagccatg gcggcacccg cacgacaagc
     8041 gcggcttaca ccaatgacgg caccgctggc ggtatcgtcg gcatctccat cgtcacaact
     8101 ccagccgaga ccgcgaagca cggctacatc ggccagctca ccgaagtcac cgcgctgcaa
     8161 ctcgccgcat cgccgacgac ggtgaacgaa ggcaccacac gccagctcac cgccaccgcc
     8221 acgctcgatg atgccacgac ctcggcgctg ctcgccaccg atgtgtcctg gagtgtgcaa
     8281 agcggcccgc tcaccagcat cagcaccagc ggcctcgcca ccgcaggcat cgtgtatcaa
     8341 aacaccgccg ctaccgcgca gggcctgtat tccggcatca ccggcacgtt gaacctcacc
     8401 gtcatcaaca cgaacaccga cgacatccca ggctactcgg gcgatggcct cgatgatgcc
     8461 tggcaggtgc aatacttcgg tctcaacaat ccgctcgccg ccccgctgct cgatcccgat
     8521 ggcgacgggc acacaaatgt gttcgagaac ctcgccggca ttgatcccac cagcgccgcc
     8581 agcttcttcc gcgtgacgca gatcagcatc agcggcggtg tcttcaacat gaccttcccc
     8641 accgtcccgg gacgcagtta ccagctccaa tcctcctcca acctcgccaa tccctggatc
     8701 aacatcggag cagcgaccgc aggcaccggc ggcatggtga ccctgcccgt ggatgtcacc
     8761 ggccagacga agcacttctt ccgcgtctcg gtgggcgctc cgtgagaaga tcgtaggccg
     8821 gatgtgttcg gtgaggtgag caacgccaac acggaggagc tgcttcaccg aactatccgg
     8881 ccggtttgga ggtgtgcgga ggcgagggcg cattcgtgcg ctcaaagaag ccggatagtt
     8941 tggcgaagag cgctggaggc gaaatgacgc gttctccgcc aaacacatcc ggcctacgac
     9001 atggcggtag gccggatgtg ttcggtgccg tgagcatcgc taacacgaag gagctgcttc
     9061 accgaactat ccggctggtt tgggggtatg cggggggtga aagagcattc gtgcgctcaa
     9121 agagccggat agtttggcga agagtgctgg aggtgaaatg acgcgccctc cgccaaacac
     9181 atccggccta cgatgggctg gcgccttgaa atcgtgcttg cgagtgcttc gtgagcgttt
     9241 ccactgtgcg catggcctgg ctgattcctg cgcacattcc gctgccggag gactttgtgg
     9301 gatttgatcc tgaaagctcg ctgacgaagg attttgaagc ctatgagcgg catctgccgc
     9361 actggcgggt gccgggctgc tgctacgttc tcacgtttcg gctggcggat tcgatcccgg
     9421 tggcggtgat ggtggaaatg caggccgagg cggaggcatg gcaaaagcgg ctggctgagg
     9481 cggctcagcg tcacggaggg aagctgccgc cggaggaact tgccgcctgg caggagttcc
     9541 agcaggtgcg gatgcggaag ctggaatcgc tgctcgatga aggccgtggc gagtgcctgt
     9601 tgcggcaccc ggagcatcag cagccgctcg tggaggcgct gcatcacttt gaaggctccc
     9661 gctgcgagat gctggcctat gccatcatgc cgaatcacgt ccacgttttg tgccgtccgc
     9721 tcggtgagca cgcgctggaa agcctgacac gctcctggaa gcgtcacagc tcggatcgca
     9781 ttcatcgtcg gctcggcagg tcgggttcgc tatggcagga ggagagcttt gatcggatta
     9841 tccgcgatgc ggcgcactat cagcaggcgg tgcgctacat cgccaagaat ccgctgcggg
     9901 caggtctcgt gccgcccgag gcagtagtgt ggttgcaccc tcgcatcgtg gcggcgaatg
     9961 cggggtagca gccgagccgt aggccggatg tgttcggtgc cgtgagcatc gccaacacga
    10021 aggagctgct tcaccgaact atccggctgg tttgggggta tgcggaggtg aaagagcctt
    10081 cgtgcgctca aagagccgga tagtttggcg aagagcgctg gaggcgaaat gacgccttct
    10141 ccgccaaaca catccggcct acgacatggc ggtaggccgg atgtgttcgg tgaggtgagc
    10201 atcgccgaca cagaggagct gcttcaccga actatccggc tggtttggcg gtaggcggag
    10261 gtgaaagagc cttcgtgcgt tcaaagaagc cggatagttt ggcgaagagt gctggaggcg
    10321 aaatgaagcg ttctccgcca aacacatccg gcct
//
LOCUS       DAOMYJ010000189        10337 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Prosthecobacter sp. isolate AMR_MDS_5629
            NODE_2159_length_10337_cov_22.863840, whole genome shotgun
            sequence.
ACCESSION   DAOMYJ010000189 DAOMYJ010000000
VERSION     DAOMYJ010000189.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867850
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prosthecobacter sp. (wastewater metagenome)
  ORGANISM  Prosthecobacter sp.
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Verrucomicrobiaceae; Prosthecobacter.
REFERENCE   1  (bases 1 to 10337)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10337)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.30
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:34:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,742
            CDSs (total)                      :: 4,684
            Genes (coding)                    :: 4,676
            CDSs (with protein)               :: 4,676
            Genes (RNA)                       :: 58
            tRNAs                             :: 55
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10337
                     /organism="Prosthecobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2159_length_10337_cov_22.863840"
                     /isolate="AMR_MDS_5629"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1965333"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..435
                     /locus_tag="PLP58_16760"
     CDS             <1..435
                     /locus_tag="PLP58_16760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ10231.1"
                     /translation="IAASGRTPFVWGALREAGQRGAFTALLCCNPAVKKAAKKLAGTP
                     WQPRVVIAPDVGPEVLTGSTRLKAGTATKLVLNMITTLAMTKAGKVISNLMVDLNPSN
                     VKLRDRAERILVELTGRPRSDARAALQACGWVVKDAYERLRS"
     gene            622..1533
                     /locus_tag="PLP58_16765"
     CDS             622..1533
                     /locus_tag="PLP58_16765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ10232.1"
                     /translation="MKTALTLLLVCLAASARGQQVHQVEMMRIHYQTEGQHAVDAADS
                     NENGIPDQVEDIMTQTLAARMMFVEVLGFPDPLATERFKTASFLDIHLRHKDVLKTNG
                     VAFDELQRFNKPGDPKGALSICFNVATSVKAPANQTPAHEFFHLIQNGATFFKNRWFT
                     EGTARWSERALGAGGLGPARILSSWPPPEARMAEIAAMAYEASEHFWNPLAARLDKRG
                     LLPETPALEKLRAMRYTDGSPVLKDEELAGWEFIREALLELGRVDDEAFRALGYDRWS
                     EENQRSAKNDAFIFRAVFEVVKRREAR"
     gene            1561..2619
                     /locus_tag="PLP58_16770"
     CDS             1561..2619
                     /locus_tag="PLP58_16770"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HRJ10233.1"
                     /translation="MNLPRTTALLSIFTLAFTAGARDTLDIYWIDSEGGGSTLVVTPA
                     GESVLIDTGNPGGRDAARIHKVATETAGLKRLDHVVITHFHIDHFGGLAELARMMPVG
                     ALYDKGITEESPDSGRNQARWALTSRPYRDAPVEKRVVLKPGDAVPLRRVEGGVPLSL
                     RCLGANQEFIPPAADAPATPDCGSVPDKEPDLSDNANSVVLLLGFGGFRFFDGGDLTW
                     NIEARLVCPLNRAGRVDLYQVNHHGLDSSNHPLLLRALDPVVAVFNNGPRKGTSQTAF
                     DSLRGAPSLKAIYQVHENVREDRHNNTEKERIANAGDTGEECAGHFIHCSVSADGGSY
                     TLHVPATGHRETFQTRAR"
     gene            complement(2632..5307)
                     /locus_tag="PLP58_16775"
     CDS             complement(2632..5307)
                     /locus_tag="PLP58_16775"
                     /inference="COORDINATES: protein motif:HMM:NF025670.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ10234.1"
                     /translation="MKHLILLFLTILPPAAALRAQSSQTIKLHPQSKTPASLSQFGGA
                     AAMNDRWIIIGEYGNNQGATAAGAAHVFDAVTGKHLRHIVNPKPQDFALFGVSVALSG
                     DHLLIGAIGGGSNGQGEAYLYHAGTGALLRTLEAPSGSMEFGVSVALSGKTAAVGDQD
                     ADLGSGEKGAVFVYPDVLANQAPQVIVPADAMPGDEWGMSVALSGRFLLGGSPSHDME
                     KGAAYLFDLGTGAQLRKFTGSGNDNLGRRVVIAGGRAVLSAPGGDWPVADAGIVRVYD
                     MQSGIFAWNLAAPDGHAGCGHGFSIAASTDHVLVGAPLKTEPNSGVAATGSAYLYDVR
                     SGHLLKQIRLPARKTQDFTGYAVALNRGNALVTSLLADETGPNRGLAVLLTGQAARLG
                     AHSLAVRGASAPGIVDARHGKFTERAINPSGYTVFCGSLEGAGASGGKTAGVFSSMTD
                     GSYADLALTAGMDLTSLGMAGVKAVSFQNPVCNSFSMGVFQAKLAGSGVSPANNLLLL
                     ADNGTEVYPLKRTGSSDPALGNAVIASIKQVLQGSKNDLGPAVLVHLKTGAGGVDKSN
                     DSTLLALKTKGDVQAFAREGNLSPPGDNFGQIHRAALAVDQAVFCADLISPKLTNQGV
                     FILNTTTNAKEMLLRKGDAAPGVTGAVISAFLGETVDQGNCVLMRGLITGQSITPAQK
                     ECLWRMPIGGMASLVARTGTQVQGMGGGITWSKFIQYWTVGGTQVLVHGKIKGPGVAP
                     ATDQVLWLLQEDGSHQVLLRKGDPLPDCGNLRVSVIQQIAVNTSHGRYTVRASLLGAP
                     KDGDQVLLAGRTLGTSIALSSLRQPFPRLRKGMAYDGGDGVISKLAGFSWTPLLPGPT
                     GAGANGQAQALNFQNQMVLDLGFANGINEVAVLNP"
     gene            5571..7772
                     /locus_tag="PLP58_16780"
     CDS             5571..7772
                     /locus_tag="PLP58_16780"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HRJ10235.1"
                     /translation="MRSPFSAVLLACLLCPPFCGIRGQAAEPQRLTVGQVLKRNQSNI
                     PVRVSGVVTAADPVRIFIQDETGGIGIFRAALRGQVEAGDLVEVSGATTGSGAGLGIS
                     GQSLVKTGKAELPKPQAISAERLEASDARHQRVRIAGTVHEVAASGEVQILQVQSGNA
                     SFMVMWQASPPEQPDRVLAPRLDLLDADVELEGVAMPQFSPAGLRSGFRLFLASSEPP
                     HLKVLRAGSSDVFTRPLRTLASLKGLKAHDNQRWLVRGVVTYWSDAGWFHLQDGTGTG
                     RGNNAHFLLKGIGWPYRTGRSEPNLKPGDEIELVGMPVITAGGHVSLSRCEWRVTGHV
                     EPPPFAPASAEQILKADMEGASVSITGRVVDVEITTDYQGFAVHTLWMESGETGFAAM
                     VQKRRQGEVPVQPGQYARVEGVVTTSPGVIGKAAFRINVNDFPAIRAVPPPPFWQTVN
                     LMRWLGAAGGVIGLFFIWILMLRRQVAAQTAQLRDNARRLEDQLEQERELSEARSRFV
                     FTVSHEFRNPLAAIMSCSDVLQRLNGRMSQEEHAHQITGIQQNVRRMADMMEEVLLLG
                     RAESGRLPCETRPLDLAAFCRKLADQILSASAGRCPVDLAVTPGLPPLMLDAGLLQHI
                     AGNLLGNAVKYSPAGMSVEFRADYQNGVCALTIRDRGPGIPTLDRPRIFEPFHRGSNV
                     DKTAGTGLGLAIAERCARAHGGGITCESDSGEGTTFTVRIPCEAASDPTPP"
     gene            7769..8383
                     /locus_tag="PLP58_16785"
     CDS             7769..8383
                     /locus_tag="PLP58_16785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007415880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HRJ10236.1"
                     /translation="MKILIIEDEPLTRENIALILRMEGYETRTAADGREGIASALREL
                     PDLILCDITMPDTDGHGVLRQVRAAEATRATPFIFLTARGDRSDLRQGMNLGADDYLT
                     KPASASEILEAIRARLEREKLREHNGFHPDFSSHDPLLALGLTAREAEVLLWIAQGKS
                     NGDIGAILGATENTVKKHAQHIFTKLGVDGRNAAALVALEKLSA"
     gene            8592..>10337
                     /locus_tag="PLP58_16790"
     CDS             8592..>10337
                     /locus_tag="PLP58_16790"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF012492.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HRJ10237.1"
                     /translation="MEKHTFAELGLTPELLKAIEAQGFEQPSPIQAQAIPVALTGRDV
                     VGQSQTGSGKTLAFGLPAVQRIDAANRAVQVLVMCPTRELAMQVCAEIHKVASFKDGV
                     RATPVYGGASYDRQIRALQAGSQIVVGTPGRILDFVDRGTLKLDSLQTLVFDEADEML
                     DMGFADEIERLMEAVPAERQTIFFSATFDPRIRRLIDRYTKDAATITIEQKAMTVPTI
                     EQRYYEVQGRSKTEVLCRVLDMDSPRLTIVFANTKKAVDDVTDALVGRGYSADRLHGD
                     LNQMMRDRVMRNFRTGTIEVLVATDVAARGLDVNDVDLIVNYDLPYDTEDYVHRIGRT
                     GRAGRKGSAVSLVAGREIYLMQRIQRFTNAKVTRAKVPSQEEVEATRVDKTFEKLKAT
                     LEGGEFTKHEHTVQRLLDAGFETTDVISAVLDLWMKESSRETEQILEDRQTAKAQAPR
                     KEFTPRESGPPVDEGPSRSDVPPRDDYGPAPMRSRSFVQKGPRSGMVRLFINVGGMDN
                     ARPGDIAGMIYNTSQIPSGSLGTIDVFEKCSYVEVPEDHVQTVMESVGGAEFRGRAVR
                     MDVADRQDGAGAGGDR"
BASE COUNT         1851 a         3358 c         3371 g         1757 t
ORIGIN      
        1 atcgccgcca gcggacgcac gccgtttgtc tggggcgcgc tgcgcgaggc cggccagcgc
       61 ggggcgttca ccgcgctgct gtgctgcaac cccgcggtga agaaggcggc gaaaaagctg
      121 gcgggcacgc cctggcagcc gcgggtggtc atcgcgccgg atgtgggacc ggaggtgctg
      181 accggctcca cgcgcctgaa ggcgggcacg gccaccaaac tcgtgctgaa catgatcacc
      241 accctggcaa tgacgaaggc gggcaaggtg atcagcaacc tcatggtgga cctgaacccc
      301 tccaacgtga agctgcgcga ccgcgccgag cgcatccttg tggaactcac gggacgcccc
      361 cggtccgacg cccgcgccgc gctgcaagcc tgcggctggg tggtgaagga tgcctatgag
      421 aggctgcgct cgtgagttgg agtttggcgt tggagtttgg cgttggagtt caagctttag
      481 cttgttcacc gacctcccgc agcggagcgc gctcccgtgt cgcctaaagg cttcactcca
      541 acggcttcac tccaacgact tcactccaat aacgcgcaac aacgcggccg cagtctcgta
      601 aggctcgaca catcaactgt catgaaaacc gccctcactc ttctgctcgt ctgcctcgcc
      661 gccagcgcgc gaggtcagca ggtgcatcaa gtcgaaatga tgcgcattca ttaccaaacg
      721 gaggggcagc acgcggtgga cgccgcggac tcgaatgaaa acggcatccc ggaccaggtg
      781 gaggacatca tgacgcagac gctggcggcg cggatgatgt ttgtggaggt gctcggcttc
      841 cccgatcccc tcgccacgga gcgcttcaag acggcgtcgt tcctcgacat ccatcttcga
      901 cacaaggatg tgctcaaaac caacggcgtg gcctttgacg agctgcagcg cttcaacaaa
      961 cccggtgatc ccaagggcgc gctgagcatc tgcttcaatg tggcgacctc ggtgaaggcg
     1021 ccggcgaatc agacgcccgc gcatgagttc ttccatctca tccagaatgg agcgaccttt
     1081 ttcaagaacc gctggttcac cgaaggcacc gcgcgctggt ccgagcgcgc cctcggcgcg
     1141 ggcggactgg gaccggcgcg cattttgtca tcatggcctc cgccggaggc gcgcatggcg
     1201 gagatcgccg ccatggccta cgaggcatcg gagcacttct ggaatccgct cgccgcgcgc
     1261 ctggacaagc gcggcctcct gccggaaacg cccgcgctgg aaaagctgcg cgccatgaga
     1321 tacaccgacg gcagcccggt gctcaaagac gaggagctgg caggatggga gttcatccgc
     1381 gaggcgctgc tggagctcgg ccgcgtggat gacgaggctt tccgcgcact cggctacgac
     1441 aggtggtccg aggaaaacca gcgctccgcg aaaaacgacg ccttcatctt ccgcgcggtg
     1501 tttgaggtgg tgaaacgccg ggaagcgcga taaaaccggc ggattatttc ttctccaatc
     1561 atgaacttgc cacgaaccac ggctctgctt tccatcttca cgctcgcctt caccgccggc
     1621 gccagggaca cgctcgacat ttactggata gactccgagg gcgggggcag cacgctggtg
     1681 gtgacgccgg cgggtgagtc ggtgctgata gacacgggca atccaggcgg gcgcgacgcg
     1741 gcgcgcattc acaaggtggc gacggagacg gcggggctga aaaggctgga tcatgtggtg
     1801 atcacgcact ttcacataga ccatttcggc ggcctggcgg agctggcgcg gatgatgccg
     1861 gtgggcgcgc tttatgacaa gggcatcacc gaggaaagcc cggacagcgg gcgcaaccag
     1921 gcgcgctggg cgctgacgag ccggccctac cgcgatgcgc cggtggagaa gcgcgtggtt
     1981 ttgaagccgg gcgatgcggt gccactgcgc cgggtggagg gcggcgtgcc gctgagcctg
     2041 cgctgcctgg gcgcgaacca ggagttcatc ccgcccgccg ccgatgcgcc cgccacgccg
     2101 gactgcggca gcgtgccgga caaggagccg gacctctccg acaatgcgaa cagcgtcgtg
     2161 ctgctgctcg gctttggcgg cttccgtttc ttcgatggcg gagatctcac ctggaacatc
     2221 gaggccaggc tggtctgccc gctgaaccgc gcgggcaggg tggacttgta tcaagtgaat
     2281 caccacggcc tcgactccag caaccacccg ctgctgctgc gcgcgctgga cccggtggtg
     2341 gcggtcttca acaacggacc gcgcaagggt acgagccaga ccgcctttga ctccctgcgc
     2401 ggcgcgccga gcctgaaggc catctaccag gtgcatgaaa acgtgcgcga ggaccgccac
     2461 aacaacacgg aaaaggagcg catcgccaac gcgggtgaca cgggcgagga atgcgccggc
     2521 catttcatcc actgctcggt gagcgccgac ggcggcagct acacgctgca tgtgcccgcc
     2581 acgggtcacc gcgagacttt tcagacgcgg gcacggtgag cggcggcggc ctcatgggtt
     2641 caagacggcc acctcgttga ttccgttggc gaagccgagg tcgagcacca tctggttttg
     2701 gaagttgagc gcctgggcct ggccgttcgc gcccgcgccg gtggggcctg gaaggagcgg
     2761 cgtccatgaa aagccggcca gtttggaaat gacgccatcg ccgccgtcat aggccatgcc
     2821 tttgcgcagg cgcgggaagg gctggcggag ggaggacagg gcgatgctgg tgccgagtgt
     2881 gcgtccggcg agcaggacct ggtccccgtc tttcggggcg ccgagcaggg atgcgcgcac
     2941 ggtgtagcgc ccatggctgg tgttgacggc gatctgctgg atgacgctga cccgcaggtt
     3001 gccgcagtcg ggcagcgggt cgcctttgcg cagcagcacc tgatgactgc cgtcctcctg
     3061 caggagccag agcacctggt cggtggcggg cgcgacaccc ggacctttta tttttccatg
     3121 caccagcacc tgggtgccgc caacggtcca gtattggatg aactttgacc atgtgatgcc
     3181 gccgcccatg ccctggactt gagtgcctgt gcgcgccact agcgatgcca tgccgccaat
     3241 gggcatgcgc cacaggcatt ccttttgagc tggagtaatg ctctggccgg tgatgagacc
     3301 gcgcatgagg acgcagttcc cctgatccac cgtctctccc agaaaagcgc tgatcaccgc
     3361 gcccgtgaca ccgggggccg cgtccccttt gcggagcagc atctccttgg cgttggtggt
     3421 tgtgttcagg ataaagaccc cctggttggt gagcttcggc gaaatcagat ccgcgcagaa
     3481 caccgcctgg tccaccgcca gcgcggcgcg gtggatttgc ccgaagttgt cacccggtgg
     3541 cgagaggttg ccctcgcgtg caaacgcttg aacgtccccc ttggttttga gggcgagcag
     3601 ggtgctgtcg ttgctcttgt ccacaccgcc agcgcctgtc ttcagatgca cgagcaccgc
     3661 cgggccgaga tcgttttttg agccttgcag cacctgtttg atggatgcga tgacggcatt
     3721 gccaagcgcg gggtcggagg agccggtgcg cttgagcgga tagacttccg tgccgttgtc
     3781 agccagcagc agcaggttgt ttgcagggga gacgccggag cccgcgagct tcgcctggaa
     3841 gacgcccatg ctgaagctgt tgcagaccgg attctgaaac gaaaccgctt tcacacccgc
     3901 catgcccagc gaagtcagat ccatgccggc agtcagcgcc aggtcggcat aagagccatc
     3961 cgtcatggag ctgaaaacac ccgcggtttt gccacccgac gcgcccgcgc cctccaggct
     4021 gccgcaaaaa acagtgtagc cagacgggtt gatggcgcgc tccgtgaatt tgccatgacg
     4081 cgcatccacg atgccgggcg cggaggcgcc gcgcaccgcc agactgtgcg cgccaagtct
     4141 ggccgcctgg ccggtcagca gcacggctaa gccgcggttc ggacccgtct cgtcagcgag
     4201 cagggaggtc acgagcgcgt tcccccggtt cagggccacc gcgtagccgg tgaaatcctg
     4261 cgtcttcctc gcgggcaggc ggatctgttt gagcaggtgg cctgatctca catcgtaaag
     4321 ataggcgctg cccgtggcgg cgaccccgct gttcggctcc gtcttcaatg gcgcgccgac
     4381 caggacgtga tctgtgctgg cggcgatcga aaaaccgtgt ccgcagcctg catggccgtc
     4441 gggggcggcg agattccagg caaagatgcc gctctgcatg tcatagacac gcacgatgcc
     4501 cgcatccgcc accggccagt cgcctccggg cgcgctgagc accgccctgc cgccggcgat
     4561 gacaactcgg cggcccaggt tgtcattgcc tgagccggtg aatttgcgca gttgcgcgcc
     4621 cgtgccgaga tcgaagagat aggcggcgcc tttttccatg tcatgactcg gcgagccgcc
     4681 gagcagaaag cgcccgctca aggccacgct catgccccat tcatctcctg gcattgcgtc
     4741 cgccggcacg atcacctgcg gagcctgatt cgccagcaca tcaggataga caaacaccgc
     4801 gcctttctcg cccgagccga gatcggcgtc ctgatcgccg accgcggcgg tctttccaga
     4861 gagcgccacg ctgacgccaa actccatgct gccggagggc gcctccaggg tgcgcagaag
     4921 agcgcccgtg ccggcatgat aaagataggc ttcgccctgg ccgttgcttc ccccgccgat
     4981 ggcgccgatc agcaggtggt ctccgctcag ggccaccgag actccgaaga gagcgaagtc
     5041 ctgcggcttg ggattgacga tatgccgcag atgcttgccc gtcaccgcgt cgaagacatg
     5101 cgcggccccc gccgccgtcg caccctggtt gttgccatat tcgccgatga tgatccatct
     5161 gtcattcatg gcggcggctc cgccgaactg gctgaggctt gcgggagtct tgctttgcgg
     5221 atgcaatttg atcgtctgcg agctttgcgc gcggagggcc gcggcaggcg gaaggatggt
     5281 gagaaaaagc aggatgagat gtttcatggc ggcgatggtg tgacggtttc accaccagtc
     5341 tgcggcaata tgcttcagca gggtatggca ctcctggatt atgcagcttg tgcctgatgc
     5401 gcggattcac cgagaccgct gaatgcgctc tgaacttgga aaaccgaggg gagcgagcgg
     5461 cacacctcat ggagagtccg cgcgttgaaa agccagccct gttctacttt ggtggtatga
     5521 cacagcaaat tgtcttgagc gtttccggcg cggacgtgaa tcttgcggcc atgcgctccc
     5581 cgttttcagc cgtgctgctg gcgtgcctgc tctgcccgcc cttttgcggc atcagaggac
     5641 aggcggctga gccgcaacgg ctgacggtgg ggcaggtgct caagcgcaat cagtcgaaca
     5701 tcccggtcag ggtaagcggc gtggtgaccg ccgctgatcc agtgcggatc ttcatccagg
     5761 atgaaacagg aggcatcggc attttccgcg cggcgctccg cgggcaggtg gaggctgggg
     5821 atctcgtgga ggtcagcggc gccaccacgg gcagcggggc gggcctgggc atcagcggcc
     5881 agtccctggt caagactggc aaggcggagc tgcccaagcc acaggcaatc agcgcggaaa
     5941 ggctggaagc ctccgatgcc aggcaccagc gcgtgcgcat tgccggcaca gtgcatgaag
     6001 tcgcggccag cggcgaggtg cagatcctgc aggtgcagtc agggaacgcc tctttcatgg
     6061 tcatgtggca ggcctccccg cctgagcagc cggacagggt gctggcgccg aggctggacc
     6121 tgctggacgc ggatgtcgag ttggagggtg ttgccatgcc ccagttttcc ccagcgggat
     6181 tgcgcagcgg tttccgtctc tttctggctt cgtcggagcc gccgcatttg aaagtgctgc
     6241 gggcggggtc ttccgatgtg ttcacacgcc ctttgcgcac cctggccagc ctgaaggggc
     6301 tgaaagccca tgacaaccag cgctggcttg tgcggggagt cgtcacttac tggtcggacg
     6361 ccggctggtt ccatcttcaa gacggcaccg gcactggccg cggaaacaac gcgcatttcc
     6421 tgctcaaagg catcggctgg ccttaccgaa caggccgctc ggagccgaac ctgaagccgg
     6481 gagatgagat cgagctggtg ggaatgccag tcatcaccgc cggaggccat gtgtcccttt
     6541 cgcgttgcga gtggcgcgtg accgggcatg tggagccgcc ccccttcgcg ccggcttcgg
     6601 cggagcaaat tctgaaagcg gacatggagg gcgcgtccgt ttcgatcacg ggcagggtcg
     6661 tggatgtgga gatcaccacg gattaccagg gcttcgccgt ccacacgctg tggatggagt
     6721 cgggagagac aggcttcgct gccatggtgc aaaagcgccg ccagggggag gtgcccgtgc
     6781 agcccggaca gtatgcgcgc gtggagggcg tggtcaccac ttcgccgggc gtcatcggca
     6841 aagccgcatt ccgcatcaat gtgaacgatt ttcccgccat tcgcgccgtg cctccgccgc
     6901 ccttctggca gacggtgaat ctcatgcgct ggctcggcgc cgcgggcggc gtcatcggcc
     6961 tgtttttcat ctggatcctc atgctgcgcc gccaggtggc cgcgcagacg gcgcaactgc
     7021 gtgacaacgc ccggcggctg gaagatcagc tcgagcagga aagggagctc agcgaagcgc
     7081 ggagccgctt cgtcttcact gtcagccatg aattccgcaa tccgctggcc gccatcatgt
     7141 cctgcagcga tgtgctgcag cggctgaacg gcaggatgag ccaggaggag cacgcgcacc
     7201 agatcaccgg catccagcaa aacgtgcgcc gcatggctga catgatggag gaggtgctgc
     7261 tgctgggacg cgcggagtcc ggccgcctgc cgtgcgagac acgcccgctc gatctcgccg
     7321 ccttttgccg gaagctggcc gaccagatcc tctccgccag cgccggacgc tgcccggtgg
     7381 acctcgcggt gacgccgggg ctgccgccgc tcatgctgga tgccgggctg cttcagcaca
     7441 tcgcgggcaa cctgctcggc aacgcggtca aatactcgcc cgccgggatg agcgtggaat
     7501 tcagggcgga ctatcaaaat ggggtgtgcg ccctcaccat ccgcgaccgc ggccccggca
     7561 tccccacctt ggaccgccca cgcatttttg agcccttcca ccgcggcagc aatgtggaca
     7621 aaaccgctgg caccggcctg gggctcgcca ttgccgagcg ctgcgcgcgc gcccatggcg
     7681 gcggcatcac ctgcgagagc gactccggcg agggcacgac cttcaccgtg cgcatcccgt
     7741 gcgaagccgc cagcgaccct actcccccat gaaaatcctc atcatcgaag acgagccgct
     7801 cacacgcgaa aacatcgccc tcatcctgcg catggagggc tatgagaccc gcaccgccgc
     7861 cgatggaagg gagggcatcg ccagcgcttt gcgcgagctg cccgacctca ttttgtgcga
     7921 catcaccatg cccgacaccg acggccacgg cgtgctccgc caggtgcgcg ccgccgaggc
     7981 cacgcgcgcc acgcccttca tcttcctcac cgcgcgcggc gaccgctccg acctgcgcca
     8041 gggcatgaac ctcggcgccg acgactacct caccaagccg gccagcgcct cggaaatcct
     8101 cgaagccatc cgcgcgcggc tcgaacgcga gaaactccgc gagcacaacg gctttcaccc
     8161 tgatttcagc tcccacgatc ctctgctggc gctgggcctc accgcgcgcg aggcggaggt
     8221 gctgctctgg atcgcccaag gcaagtccaa cggtgatatt ggcgccatcc tcggcgccac
     8281 ggaaaacacc gtgaagaagc acgcgcagca catcttcacc aagctcggcg tggacggccg
     8341 caacgccgcc gcgctcgtgg cgctggagaa actcagcgcg tgagcttgaa tccgcgcttg
     8401 aatccgccct tgcgtttcaa aaaatgtgtg ggattcactc tgccggtttt ttcgccgctt
     8461 ccccgcttgg ggccgaagtt tgacatgtag cccgcccgtt tggcggttcc ccgtcccgct
     8521 catgcgggac gcttttactg cgacaggttg cccgttattt ggaagccaac ctgccggttc
     8581 gtaaatcaga catggaaaag catacattcg ccgagcttgg cctcacgccc gagcttctca
     8641 aagccatcga agcccagggc tttgaacaac cctcccccat ccaggcgcaa gccatccccg
     8701 tggccctcac cgggcgggat gtcgtgggcc agagccagac cggctcgggc aaaacgctcg
     8761 ccttcggcct gcccgccgtg cagcgcattg acgccgccaa ccgcgccgtg caggtgctcg
     8821 tcatgtgccc cacgcgcgag ctggccatgc aggtctgcgc ggagattcat aaagtggcct
     8881 ccttcaagga tggcgttcgc gccacgccgg tctatggcgg cgcttcctat gaccgccaga
     8941 tccgcgcgct ccaggccggc tcgcaaatcg tggtcggcac accggggcgc atcctcgact
     9001 tcgtggaccg cggcactttg aagctcgaca gccttcagac gctcgtcttc gacgaggcgg
     9061 atgaaatgct cgacatgggt tttgccgacg agatcgagcg cctcatggag gcggtgcccg
     9121 ccgagcgcca gaccatcttt ttctccgcca ccttcgaccc gcgcatccgc cggctcattg
     9181 accgctatac gaaggacgcg gccaccatca ccatcgagca gaaggcgatg acggtgccca
     9241 ccatcgagca gcgctactac gaggtgcagg gccgcagcaa gaccgaggtg ctttgccgcg
     9301 tgctggacat ggacagcccg cgcctgacca tcgtcttcgc caacacgaaa aaggccgtgg
     9361 atgatgtcac cgacgcgctc gtgggccgcg gctactccgc cgaccgtctg catggcgacc
     9421 tgaatcaaat gatgcgcgac cgcgtgatgc ggaacttccg caccggcacc atcgaggtcc
     9481 ttgtggccac ggatgtggcc gcgcgcgggc tggatgtgaa cgacgtggac ctgatcgtga
     9541 actacgacct gccctacgac accgaggact acgtccaccg catcggacgc acgggccgcg
     9601 ccgggcgcaa gggcagcgcg gtgagcctgg tggcggggcg tgagatttat ctcatgcagc
     9661 gcatccagcg cttcaccaat gccaaggtca cacgcgccaa agtgccctcg caggaggagg
     9721 tggaggccac gcgcgtggac aagacctttg aaaaactcaa ggccaccctg gagggcggcg
     9781 agttcaccaa gcacgagcac acggtgcagc gcctgctcga cgccggtttt gaaaccacgg
     9841 atgtcatcag cgccgtgctg gacctttgga tgaaggagtc ctcgcgcgag acggagcaga
     9901 tcctcgaaga ccgccagacg gcgaaagcac aggcgccgcg caaggagttc actccgcgcg
     9961 agtccggtcc gccggtggat gaggggccct cccgctccga tgtgccaccg cgtgacgact
    10021 acggccccgc gccgatgcgc tcgcgctcct ttgtgcagaa gggtccgcgc tccggcatgg
    10081 tgcggctttt catcaatgtc ggcggcatgg acaacgcgcg tcccggtgac atcgccggca
    10141 tgatctacaa cacctcgcag atcccgagcg gcagcctcgg caccattgat gtgttcgaaa
    10201 aatgcagcta cgtcgaggtg cccgaggatc atgtgcagac ggtgatggaa agcgtgggcg
    10261 gagccgaatt ccgcggacgc gccgtgcgca tggacgtggc cgaccgccag gatggcgccg
    10321 gcgccggtgg cgaccgc
//
LOCUS       DAOMYJ010000190         9990 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Prosthecobacter sp. isolate AMR_MDS_5629
            NODE_2252_length_9990_cov_20.647207, whole genome shotgun sequence.
ACCESSION   DAOMYJ010000190 DAOMYJ010000000
VERSION     DAOMYJ010000190.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867850
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prosthecobacter sp. (wastewater metagenome)
  ORGANISM  Prosthecobacter sp.
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Verrucomicrobiaceae; Prosthecobacter.
REFERENCE   1  (bases 1 to 9990)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9990)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.30
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:34:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,742
            CDSs (total)                      :: 4,684
            Genes (coding)                    :: 4,676
            CDSs (with protein)               :: 4,676
            Genes (RNA)                       :: 58
            tRNAs                             :: 55
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9990
                     /organism="Prosthecobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2252_length_9990_cov_20.647207"
                     /isolate="AMR_MDS_5629"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1965333"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..723)
                     /gene="dacB"
                     /locus_tag="PLP58_16795"
     CDS             complement(<1..723)
                     /gene="dacB"
                     /locus_tag="PLP58_16795"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: protein motif:HMM:NF014200.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004175 - endopeptidase activity [Evidence
                     IEA];
                     GO_function: GO:0009002 - serine-type D-Ala-D-Ala
                     carboxypeptidase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine
                     carboxypeptidase/D-alanyl-D-alanine-endopeptidase"
                     /protein_id="HRJ10238.1"
                     /translation="MWKNVLICLLAASLAASLILRWRDRVNDTPPAPLQTALGEALAR
                     ALGDTEAGGMALGLCVQDEAGNTIFGREARRAFIPASSLKTLTTATALELLGPDFHFQ
                     TRLLSAAPMAEGVIEGDLILRGGGDPMLSLADLSKCAAELQARGLREVRGRVLGDGGF
                     FTGSIYGDFWNWGDIGNGYGSPVSGLNLEHNRYTAVFAPALQEGGPAAFVGAQPEVPG
                     ITWLSEVRTGPEGGGDGVMIHGG"
     gene            complement(762..1133)
                     /locus_tag="PLP58_16800"
     CDS             complement(762..1133)
                     /locus_tag="PLP58_16800"
                     /inference="COORDINATES: protein motif:HMM:NF013331.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group 1 truncated hemoglobin"
                     /protein_id="HRJ10239.1"
                     /translation="MSQEPTLFDRIGGEAGIEELILAFYVKVLADPELAPFFRETSLE
                     KLHSMQREFFAMALGGPVKYTGRSLAHVHHGRGITMGHFARFTGHLVETLRDTGASQE
                     DTDAVIARIEVFANEVTGTSY"
     gene            1294..2217
                     /locus_tag="PLP58_16805"
     CDS             1294..2217
                     /locus_tag="PLP58_16805"
                     /inference="COORDINATES: protein motif:HMM:NF019474.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HRJ10240.1"
                     /translation="MSSPGRLRHALRLMLRAGLLVLVFILLLAGAGWWYLHPASEVTR
                     GIVYGQRNGRELTLEVHRPKAPNGIGVLIMVSGKWKSHPGKFRAWMAAPSLRAGHTVF
                     AVSHIAQPEASVPETVEDIHRAARFVRHHARDYGVDPRRLAVTGGSSGGHLSLMLATR
                     GGPGPADAPDPVDREDSSVQAAAVFFPVTDLLNLGPSTENDGTGGPPKSFKKSFGPEA
                     ADLEKWKLIGREVSPIFHITPRLPPVLIAHGDADTLVPLDQSERFQKRAAELGHDIRV
                     LVRPGARHGWLTIMWDVRRFAEWFNERLAAL"
     gene            2227..2940
                     /locus_tag="PLP58_16810"
     CDS             2227..2940
                     /locus_tag="PLP58_16810"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: protein motif:HMM:NF013045.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HRJ10241.1"
                     /translation="MLTRCPILHQDRHLILVNKPAGLLSHPNPDRRGEPAAFEGRYDM
                     RTECFDGPDGRVWLMHRLDQDTSGVLLGALNEAAAGKCRSAFEDGAAGKTYLALVRGN
                     PGKDGVWLDHLATTRERGRVRTAVLKGRPPNAELHFKNLSYHAGHRLSLLEIRLLTGR
                     THQIRVQAAARQHHLLGDDIYGHFELNRRLRAELSVKRLCLHATRLEIKHPASGQALR
                     AEAPLPEDFQAVLKALGLA"
     gene            complement(2991..4760)
                     /locus_tag="PLP58_16815"
     CDS             complement(2991..4760)
                     /locus_tag="PLP58_16815"
                     /inference="COORDINATES: protein motif:HMM:NF025056.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIR domain-containing protein"
                     /protein_id="HRJ10242.1"
                     /translation="MKSRPPDYPARSIFICYRRDESSDISGRIYDRLVSAINKEAVFQ
                     DIDSMPYGVDFREHAREALQNCRVVLVIIGPGWTDIRDSYGQKRLDDLADPVRIEVET
                     ALANPGALVIPVFVNGAKLKTADVPESLRPLCTRNGPELRSDATFDAVMERISSQIRA
                     ELLRPVVEEGARPVLPKRFTGIIIGAVAALALGWLGWQNYALRPGMEKKAVVLPPIGE
                     DAKPAKEEPDFAEILKGARFSVASSSKPAYELLTPDGKPLQGAKLKGERLSTESEGHG
                     VKFPTAVRQLSGQLPPDAGIMYKVRAQSLKDASENPPGPGVLVVDTEWMGQENKTLGA
                     LNDEFFSGFWSMKGAEFPADGIQLGSAAIYAGKNGVVMDGELEVECWVEDGHHGKLSG
                     VTSFTLDVEPAPWALENPKIRLLAPDGTPIEDTVVQASPAQPFDYEDPETRQKQTALH
                     GLEFLLSIKNTSGADLTPAGELDALGITYRLKAKRDGSPESDSSPLAEGGLLICEPNP
                     QGYTHHSSWQMWSLKASQLSMGQTGEVLNKFYVSHQENEKLGHILPGIHEVGVTVWRS
                     TEPGVVYYQGTLKLEVKERIPAR"
     gene            4917..6944
                     /locus_tag="PLP58_16820"
     CDS             4917..6944
                     /locus_tag="PLP58_16820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009961481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006259 - DNA metabolic process [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase/topoisomerase IV subunit A"
                     /protein_id="HRJ10243.1"
                     /translation="MPKKSAKQPAEDNSPILADSAPIEIKPVDDLYSDWFLDYASYVI
                     LERAVPHIHDGFKPVQRRIMHSLDELEDGRYNKVANVVGNTMKYHPHGDASIGDAFVQ
                     LGQKDLLIDTQGNWGNVLTGDNAAAPRYIEARLSKFALDVVFCPKVTEWAASYDGRNK
                     EPVTLPVKFPLLLAQGVEGIAVGLSCRILPHNFIELCDASIAALRGEEVNLVPDFPQG
                     GIMDASDYNQGLRGGRVRVRAKIEEGAKKNILRITEIPFGTTTGSIQDSIVAANEKGK
                     IKIARVEDNTAEFVEIVIQLPPGVDPEQTIQALYAFTDCEVSLAPNAVVIQNDKPRFA
                     GVNDLLKESALHTKGILGRELEIQLGELEEKWHFSSLEKIFIENRIYRRIEECETWDA
                     VMQAIWTGLKPHLGALKREVTDDDVARLTEIKIKRISKFNSFEADEHIRSLEKQIAEV
                     QKNLKSLTRYTIAHFERLKKTYGTGRERRTEVSSFDRVAAAEVIMANETLYLDAKEGF
                     AGTGLKREGEPVCKCSPLDDIIWFTKDGTMTVSKVAPKLHVGKNPVYLSLFKKDEDAV
                     YTLIYRNGKQGPVLAKRFRIGGITRDKEYVLTKGAPGSMVLFFARHETEAESDAQNLL
                     IHLKPALYLRTLCLKFPVASLAVKGRDSQGNIVTKHTVDRVVNERKSAAPE"
     gene            6941..8482
                     /locus_tag="PLP58_16825"
     CDS             6941..8482
                     /locus_tag="PLP58_16825"
                     /EC_number="3.5.1.4"
                     /inference="COORDINATES: protein motif:HMM:NF006006.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HRJ10244.1"
                     /translation="MKRRVLMNAGAAALLAPLAGCRRAAPKGGWTIREMRDSGWPAPD
                     LVADALRRVEALNLNGPSLRAVIETNPDAPDIARGLERALARGPLHGIPVLVKDNLDT
                     ADAMRTTAGSLALLDAPAPERDATVVARLRAAGAVILGKANLSEWANLRSPRSTSGWS
                     ARGGLTLNPHDPRRTASGSSSGSAAAVAAGMVPAAIGTETNGSIVSPAACCGIVGLKP
                     TVGLVSRAGIIPITPWQDTAGPMTRTVRDAALVLSAIAGHDRMDPFTKNAADRVLADY
                     TVDFSVDALQGARLGIVRGMCGRDGRVHGVFEEALKLLREAGAVLVDGVEIPHRREAG
                     GAAWSAMLTEFRLALNEYLGARGGPVRTLADVIAFNEARAGEELAHFGQELFIEAEQR
                     QGDDALAGARAARSQALDLAGPRGIDAALREHRLDALICATNDPAGVVNLARGDSGGR
                     TASGPAAVAGYPHLTLPMGRVDGMPVGLSFLGAAWSERRLLAFGDAFERRHPARAQET
                     CCEAM"
     gene            complement(8501..>9990)
                     /locus_tag="PLP58_16830"
     CDS             complement(8501..>9990)
                     /locus_tag="PLP58_16830"
                     /inference="COORDINATES: protein motif:HMM:NF020976.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="alkaline phosphatase D family protein"
                     /protein_id="HRJ10245.1"
                     /translation="GARKVPYGLALTFPAMPPRIPTAIVFSLACLAAGHAEVHTAQGI
                     LSGEATATSVLLQTRLTAIPGPELDREGDVPGAEGVACFEYGADPAFANAARTPWLRA
                     APDRDFIVRAHVMGLKAGTLYHCRVIAGKDEADARPGRAGSFRTLPDAAGHAPLSFVM
                     GSCMNYHSFMAGKANGGGPVTATEEDKRLGYPSFAAMSALRPAFFIGTGDIVYYDHPA
                     QAAASTLPELRRKWHEQFRFPRMTDFFAVTPAYWSKDDHDFRYNDADLRGEREPRAGT
                     GIEIFREQMPVLPADAAATPTYRTHRVHRHAQLWFVEGRDYRSPNKMPDGPDKTLWGA
                     EQRVWLQRTLKESDAPWKIIITPTPMVGPDRNSKTDNHTNLAGFKHEADSFFEWLRAE
                     GVTGVLTFCGDRHWQYHSIHPLGVEEFSVGALNDENAIRGERPGGPKTTDPEGKIKQP
                     YIYSEPTGGFLHVTLSTGTQGRAKLGIEFRDDHGKVLHSVLKRAE"
BASE COUNT         1775 a         3287 c         3210 g         1718 t
ORIGIN      
        1 gccgccgtgg atcatgacgc catccccgcc gccttccgga ccggtgcgca cctcgctcag
       61 ccaggtgatg cccggcacct ccggctgcgc gccgacgaac gcggcggggc cgccttcctg
      121 cagagccggg gcgaagacgg cggtgtagcg gttgtgctcc agattcagcc cggagacggg
      181 gctgccgtag ccgttgccga tgtcgcccca gttccagaag tccccgtaga tggagccggt
      241 gaaaaagccg ccatcgccga gaacgcgccc tcgcacctcg cgcaggccgc gcgcttggag
      301 ttcggcggcg catttggaca agtcggccag gctgagcatg gggtcgccac cgccgcgcag
      361 gatgagatcc ccttcgatga caccctccgc catcggagcc gcagacagca ggcgcgtctg
      421 aaaatgaaag tccggcccca gcaactccag cgccgtggca gtggtcagcg tcttcagact
      481 ggaggccggg atgaacgccc gccttgcctc gcgcccgaag atcgtgttgc cagcctcgtc
      541 ctgcacgcag agaccgagcg ccatgcctcc ggcctcggtg tcacccaaag cccgggccag
      601 cgcctcaccc agcgcggtct ggagcggcgc gggcggcgtg tcattgacgc ggtcccgcca
      661 gcgcaggatg aggctcgcgg caagggacgc cgccagcagg cagatgagaa catttttcca
      721 cacgcgcgga cgttaaagca ccgcaggccg cgagggcaaa ctcaatacga tgtgccggtg
      781 acctcgttgg caaagacctc gatgcgcgcg atgaccgcgt ccgtgtcctc ctggctggcg
      841 ccggtgtccc tcaacgtctc caccagatgg cccgtgaacc tggcgaagtg gcccatggtg
      901 atgccgcgcc cgtgatgcac atgcgccaga gagcgccccg tgtatttcac cggcccgccc
      961 agcgccatgg cgaaaaactc gcgctgcatg gagtgcagct tctccaggga ggtctcgcgg
     1021 aaaaacggcg cgagttccgg gtcggcgagc actttgacgt agaaggcgag gatcaactcc
     1081 tcaataccgg cctcgccgcc gatgcggtca aacaatgtgg gttcctggct catggtcacg
     1141 tcaaacattc agcacaaaga atgccttcct ccacaaaaca acaagtcccg atcttgcgca
     1201 tgactgaccg cttccttcgc agccatcgcg ggcgtccctc ctgttcgaga attgccgctt
     1261 ggcaaaaccg cccgccactg aataaatcac cccatgtcct cacccggccg ccttcgccat
     1321 gccttgcgcc tcatgctccg ggcagggttg ctcgtcctcg ttttcatcct cctgctcgca
     1381 ggcgcggggt ggtggtattt gcatcccgcc agcgaggtca cgcgcggcat tgtgtacggg
     1441 cagcgaaacg gcagggaact caccctggag gtgcatcgtc cgaaggcgcc gaacggcatc
     1501 ggcgtgctga tcatggtcag cggcaaatgg aagtcccatc ccggcaaatt ccgcgcctgg
     1561 atggccgcgc cctccctgcg cgcgggccac accgtttttg ccgtatcgca catcgcccag
     1621 cccgaagcca gcgtgccgga gaccgtcgag gacatccatc gcgcggccag gttcgtgcgc
     1681 caccacgcgc gcgactatgg cgtggacccg cgccggctcg ccgtcaccgg cggcagctcc
     1741 ggcggacatc tcagcctcat gctcgccacg cgcggcggtc ccggccctgc ggacgcgccc
     1801 gaccccgtgg atcgcgagga cagctccgtg caggccgccg ccgtcttttt ccccgtcacc
     1861 gatttgctga atctcggccc ttcaactgag aacgacggca ctggcggccc gccgaagagc
     1921 ttcaaaaaat ccttcggccc cgaagccgcc gacctggaaa agtggaagct catcggccgc
     1981 gaggtctcgc ccatcttcca catcactccc aggctgccgc ccgtgctcat cgctcatggt
     2041 gatgcggaca cgctcgtgcc gctcgaccag tccgagcgct tccaaaagcg cgccgccgag
     2101 ctcggccacg acatccgcgt cctcgtccgg cccggcgccc ggcatggatg gctgaccatc
     2161 atgtgggacg tgcgccgctt cgctgaatgg ttcaacgagc ggctggcagc actgtgagac
     2221 actcccatgc tcacgcgctg tcccatcctg catcaagacc ggcacctgat cttggtcaac
     2281 aaacccgccg gcctgctctc ccacccgaac cctgaccgcc gtggcgaacc cgccgccttc
     2341 gaaggccgtt acgacatgag gacggagtgc ttcgacggac cggatggccg ggtctggctc
     2401 atgcaccggc tggaccagga cacatcgggg gtgctgctag gcgctctcaa tgaggcggcc
     2461 gccggcaaat gccgctccgc ttttgaagac ggcgccgccg gcaaaaccta cctcgccctc
     2521 gtgcgtggaa atcccggcaa ggacggcgtc tggctcgacc acctggccac cacgcgcgag
     2581 cgcgggcgcg tccgcaccgc cgtgctcaaa ggccgcccgc cgaatgccga gctgcatttc
     2641 aaaaacctct cttatcatgc cgggcatcgt ctttccctgc tggagatccg cctgctcacc
     2701 ggcaggacac atcaaatccg cgtccaggcc gccgcgcgcc agcaccacct gctcggcgat
     2761 gacatctacg gccacttcga gctgaaccgc cgcctgcgcg cggagctgtc cgtcaagcgc
     2821 ctctgcctgc acgccacccg cctcgaaatc aagcaccccg ccagcggcca agccctgcgc
     2881 gccgaggccc cgctgccgga ggattttcaa gcagtgctga aggcgctggg gctggcttga
     2941 gcgtgacagg gaccgcttgt gacggggctg tgaggcatcc tggaggaatg ctaccgcgcc
     3001 gggatgcgct ccttcacttc gagcttcaag gtgccctggt agtacaccac gcccggttcg
     3061 gtggacctcc acaccgtcac accaacctcg tggatgccag gaaggatgtg gccgagcttt
     3121 tcattctctt gatgggagac atagaacttg ttcaggactt ctcctgtttg acccatggaa
     3181 agttgactcg ccttgaggga ccacatctgc caggacgaat gatgcgtgta gccttgggga
     3241 ttgggttcgc agatcagcag gcctccctct gccagcggtg acgagtcact ttccggcgag
     3301 ccgtcacgtt tcgccttgag cctgtaggtg atgcccagcg catccaattc tccagcaggc
     3361 gtcaagtccg caccgctcgt gtttttgatg ctcagcagga actccaggcc gtgcaaagcg
     3421 gtctgctttt ggcgggtttc cgggtcttca taatcaaaag gctgcgccgg tgaggcctgc
     3481 accacggtgt cctcaatcgg cgtgccatcg ggagccagga gcctgatttt tggattctcc
     3541 aaagcccagg gcgcaggctc cacatcaagg gtgaagcttg tgaccccgga cagcttgcca
     3601 tgatgcccgt cctcgaccca gcattcgact tcaagctcgc cgtccatgac cactccgttc
     3661 ttgccggcgt agatggcagc ggatcccaat tgaattccat ctgccggaaa ctccgcgcct
     3721 ttcatgctcc aaaaaccgga aaagaattcg tcattgagcg cgccgagagt cttattctct
     3781 tgccccatcc attcggtgtc caccacaagg acacccggtc cgggcgggtt ttctgaggcg
     3841 tctttgaggc tttgggcgcg caccttgtac atgattccag catcgggagg caactgcccg
     3901 gacaactgcc ggactgcggt tggaaatttc accccgtgcc cttcggattc cgtgctcaat
     3961 ctctctcctt tgagcttcgc tccttgaagg ggtttcccgt cgggagtcaa tagttcgtaa
     4021 gcgggtttgc ttgatgacgc gacgctgaat cttgctccct tcaaaatctc agcgaaatcg
     4081 ggttcctctt ttgccggttt cgcatcttcc cctatcggcg gtaaaacgac cgctttcttt
     4141 tccattccag gccgcagcgc gtagttctgc catcccagcc atcccagtgc cagggcggcc
     4201 accgcaccga tgatgatgcc ggtgaagcgt ttgggaagaa ccgggcgcgc gccttcctca
     4261 acgacagggc ggagcagctc ggcgcggatc tgggaggaaa tgcgttccat gaccgcgtca
     4321 aacgtggcat cgctgcgcag ttcggggccg ttgcgggtgc agagggggcg caggctttcg
     4381 ggcacgtctg cagtcttgag tttggccccg ttcacgaaca ccgggatgac cagcgcgccc
     4441 ggattggcca gcgcagtctc cacttcgatg cgcacagggt ccgcaagatc atccagccgc
     4501 ttctggccgt aactgtcgcg aatgtccgtc cagcccgggc cgatgatgac gagcaccacg
     4561 cggcagtttt gcaacgcctc cctcgcatgc tcgcggaagt ccacgccgta gggcatggag
     4621 tctatgtcct ggaacacagc ctccttgttg atggcgctca ccaggcggtc atagatgcgg
     4681 ccggaaatgt cgctcgattc atccctgcgg tagcagatga agatgctgcg cgcgggatag
     4741 tctgggggcc tggacttcat gcgcgccgag gttcgtttct ggcttgaggt tggcaagcag
     4801 gaatagcgcc aagccgcctg tttgcagggc attttggcag acaggctccc gtgcttgcgc
     4861 gggcgggagc gccgcctatc ctccggcctc cttccacccc cgattgattt ttccccgtgc
     4921 caaaaaaatc cgccaagcag cccgccgaag acaactcccc catcctggcc gacagcgccc
     4981 ccatcgagat caaacccgtg gatgatctct acagcgactg gttcctcgac tacgccagct
     5041 atgtgatcct ggagcgcgcg gtgcctcaca tccacgatgg cttcaagccc gtgcagcggc
     5101 gaatcatgca cagcctcgac gagctggagg acggccgcta caacaaggtg gcgaacgtgg
     5161 tcggcaacac gatgaagtac cacccgcacg gtgacgccag cattggcgac gcctttgtgc
     5221 agctcggcca gaaggacctg ctcatagaca cccaggggaa ctggggcaac gtgctcaccg
     5281 gcgacaatgc ggcagcaccg cgatacatcg aggcgcggct ctccaagttc gcgctggacg
     5341 tggtcttctg cccgaaggtg acggagtggg ccgccagcta cgacgggcgc aacaaggagc
     5401 ctgtgacgct gccggtgaag ttcccgctgc tgctggccca gggcgtggag ggcatcgccg
     5461 tcgggctttc ctgccgcatc ctgccgcaca atttcatcga gctgtgcgat gccagcatcg
     5521 ccgcgctgcg cggggaggag gtcaatctcg tgccggactt cccccagggc ggcatcatgg
     5581 acgccagcga ctacaaccag ggtctgcgcg gcggccgtgt gcgcgtgcgg gcgaagatcg
     5641 aggagggcgc caaaaaaaac atcctgcgca tcaccgagat cccctttggc accaccaccg
     5701 gcagcatcca ggacagcatc gtggccgcga acgagaaggg caaaatcaag atcgcccgcg
     5761 tcgaggacaa caccgccgag ttcgtcgaga tcgtcatcca gctcccgccc ggcgtggacc
     5821 cggagcagac catccaggcg ctctatgcat tcaccgattg cgaggtctcc ctcgcgccga
     5881 acgctgtcgt catccagaac gacaagcctc gcttcgcggg cgtgaacgac ctgctcaaag
     5941 aaagcgcctt gcacaccaag ggcatcctcg gccgggagct ggaaatccag ctcggcgagc
     6001 tggaggaaaa gtggcatttc agcagcctgg aaaagatctt catcgaaaac cgcatctacc
     6061 gccgcatcga ggaatgcgag acctgggatg ccgtcatgca ggccatctgg accggcctca
     6121 agccccacct cggcgcgctc aagcgcgagg tcaccgatga cgacgtggcg cgcctcaccg
     6181 agatcaagat caagcgcatc tccaagttca acagcttcga ggccgacgag cacatccgct
     6241 ccctggagaa acaaatcgcc gaggtccaaa aaaacctcaa aagcctcact cgctacacca
     6301 tcgcccattt cgagcggctc aagaaaacct acggcacagg ccgcgagcgc cgcaccgaag
     6361 tctccagctt cgaccgagtg gccgccgccg aggtcatcat ggccaatgag acgctctacc
     6421 tcgacgccaa ggagggcttt gccggcaccg gcctcaagcg cgaaggcgag cccgtgtgca
     6481 aatgctcgcc tcttgatgac atcatctggt tcaccaagga cggcaccatg accgtgtcca
     6541 aagtggcgcc gaagctgcat gtggggaaga accccgtgta tctgagcctg tttaaaaagg
     6601 acgaggacgc ggtttacacg ctcatctacc gcaacggcaa acagggtccc gtgctggcca
     6661 agcgcttccg catcggcggc atcacgcgcg acaaggagta tgtgctcacc aaaggcgcgc
     6721 ccggcagcat ggtgctcttc ttcgcccgcc acgagaccga ggccgagagc gacgcccaaa
     6781 acctgctcat ccacctcaag cccgccctct acctgcgcac gctgtgcctg aaattccccg
     6841 tggcctctct cgctgtcaaa ggccgagact cgcagggaaa catcgtcacc aagcacaccg
     6901 tggaccgagt cgtcaacgag cggaaatccg ccgcgccgga atgaaacgac gcgtcctcat
     6961 gaatgccggg gcagcggctc ttctggcgcc gctggcgggc tgccggcgcg cggcgccgaa
     7021 gggcgggtgg acgatccggg agatgcggga cagcgggtgg ccggcgcctg atctggtggc
     7081 ggacgcgcta aggcgcgtcg aggcgctcaa tctaaacggc ccgtcgctgc gggcggtcat
     7141 tgagacaaat ccagacgcgc cggatattgc acgcggtctt gagcgcgcgt tggcgcgcgg
     7201 accgctgcac ggcatcccgg tgctggtgaa ggacaatctg gacacggcgg acgccatgag
     7261 aaccaccgca ggctcgctgg cgctgctcga cgcgcccgcg ccggagcggg atgccactgt
     7321 ggtggcgcgc ctgcgcgcgg cgggcgcggt gattctcggc aaggcaaact tgagcgagtg
     7381 ggccaacctg cgctccccgc gctcgaccag cggctggagc gcgcgcggcg gcctgacatt
     7441 gaatccccat gatcccaggc gcaccgccag cggctcaagc tcgggctcgg cggcggcggt
     7501 ggcagcgggc atggtgcccg cggccatcgg cacggagaca aacggctcca ttgtcagccc
     7561 ggccgcctgc tgcggcatcg tgggtctgaa gccgactgtg ggcctggtca gccgcgcggg
     7621 catcatcccc atcacgccct ggcaggacac cgcagggccg atgacgcgca ccgtgcggga
     7681 tgccgcgctc gttttaagcg ccatcgcggg tcatgaccgc atggatccgt tcacgaaaaa
     7741 cgcggcggac cgcgtgcttg ccgactacac ggtggatttt tccgtggatg cgctccaagg
     7801 cgcgcggctg ggcatcgtgc gcgggatgtg cggcagggac gggcgcgtgc atggcgtctt
     7861 tgaggaggcg ctgaaactgc tgagggaggc gggcgcggtg ctggtggacg gggtggaaat
     7921 cccccacagg cgggaggcgg gaggcgcggc atggtccgcg atgctcaccg aattccgtct
     7981 ggcgctgaac gaataccttg gggcgcgcgg cgggccggtg cggacgctgg cggatgtcat
     8041 cgccttcaac gaggcgcgcg cgggggagga actggcgcat ttcggccagg aactcttcat
     8101 cgaggcggag cagcgccagg gggatgacgc actagccggg gcgcgcgccg cgcgcagtca
     8161 ggcgctcgat ctggcgggac cgcggggcat tgacgcggcg ctgcgggaac accggctgga
     8221 cgctctgatc tgcgccacga acgacccggc cggtgtggtg aacctcgcca ggggtgacag
     8281 cggcggacgc accgcgtccg gacctgccgc cgtggcgggt tacccgcatc tgacgctgcc
     8341 gatgggccgg gtggacggga tgccggtggg gctgtccttc ctcggcgcgg catggtcgga
     8401 gcgcaggttg ctggctttcg gcgatgcctt tgagcggcgt catccggcgc gcgcgcagga
     8461 gacatgctgc gaagcgatgt gaaaggcggc gcctcttgcg tcattcggcg cgcttgagca
     8521 cgctgtgcag gactttgccg tggtcgtcgc ggaactcgat tcccagcttg gcgcgtcctt
     8581 gcgtgccggt gctgagcgtg acatggagga agccgccggt gggttcggag tagatgtagg
     8641 gctgcttgat tttgccctcg gggtcggtgg tcttcgggcc gccgggacgc tctccccgga
     8701 tggcgttctc gtcattgagc gcgccgacgg agaactcctc gacgccgagc ggatggatgc
     8761 tgtgatactg ccagtgacgg tctccgcaaa aggtgaggac accggtgacg ccctccgcgc
     8821 gcagccattc aaaaaaactg tccgcctcgt gcttgaagcc ggccaggttg gtgtggttgt
     8881 cggtcttgct gttgcggtcc gggcctacca tcggggtggg tgtgatgatg atcttccacg
     8941 gcgcgtcgct ttccttcaac gtgcgctgga gccacacgcg ctgctccgcg ccccagaggg
     9001 ttttgtccgg gccgtcgggc attttgttcg gcgagcggta gtcacgcccc tcgacgaacc
     9061 agagctgcgc gtggcggtgg acgcggtgcg tgcggtaagt gggcgtggcg gcggcgtcag
     9121 cgggcaggac gggcatctgc tcgcggaaga tttcaatgcc ggtgcccgcc cgcggctcgc
     9181 gttcgccgcg caggtcggcg tcgttgtagc gaaagtcgtg gtcgtccttc gaccagtagg
     9241 ccggagtcac ggcgaaaaag tccgtcatgc gcggaaagcg gaactgctcg tgccattttc
     9301 tgcgcagttc cgggagcgtg ctggcagctg cctgcgcggg atggtcgtaa tacacgatgt
     9361 ctccggtgcc gatgaagaag gcaggcctga gcgccgacat ggccgcgaag gaggggtagc
     9421 ctaagcgctt gtcctcctcc gtggcggtga cggggccgcc gccgttggcc ttgccggcca
     9481 tgaacgaatg ataattcatg caactgccca tgacgaagga aagcggcgcg tgccctgcgg
     9541 catccggcag ggtgcggaag ctgccggcgc gtccgggccg cgcgtccgcc tcgtcttttc
     9601 cggcaatgac acggcagtga tatagcgtgc cggctttgag tcccatgaca tgggcgcgca
     9661 cgatgaagtc ccgatccggc gcggccctga gccagggcgt gcgcgcggca ttggcgaagg
     9721 cgggatcagc gccatactca aaacacgcca cgccctccgc gccgggcaca tccccttccc
     9781 tgtcgagttc gggtccgggg atggcggtaa gccgcgtctg cagcaggacg gaagtggcgg
     9841 tggcctcgcc cgagaggatt ccctgcgcgg tgtggacttc ggcgtgaccc gcggcaaggc
     9901 aggcgaggga aaaaacgatg gctgtgggaa tgcgcggcgg catggctggg aaggtcaacg
     9961 ccagcccgta aggaaccttg cgcgcgccct
//
LOCUS       DAOMYJ010000191         9971 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Prosthecobacter sp. isolate AMR_MDS_5629
            NODE_2259_length_9971_cov_19.487495, whole genome shotgun sequence.
ACCESSION   DAOMYJ010000191 DAOMYJ010000000
VERSION     DAOMYJ010000191.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867850
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prosthecobacter sp. (wastewater metagenome)
  ORGANISM  Prosthecobacter sp.
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Verrucomicrobiaceae; Prosthecobacter.
REFERENCE   1  (bases 1 to 9971)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9971)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.30
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:34:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,742
            CDSs (total)                      :: 4,684
            Genes (coding)                    :: 4,676
            CDSs (with protein)               :: 4,676
            Genes (RNA)                       :: 58
            tRNAs                             :: 55
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9971
                     /organism="Prosthecobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2259_length_9971_cov_19.487495"
                     /isolate="AMR_MDS_5629"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1965333"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1199
                     /locus_tag="PLP58_16835"
     CDS             <1..1199
                     /locus_tag="PLP58_16835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ10246.1"
                     /translation="PRLPARTRPGQFWCADPRLESIVARDDREGRWVAGPRFTEPAAG
                     EVEALRQLYLAWWERWWADWKKSHPDEDALSPRHILGDLGRWFSRERYPLHACHLQQA
                     RRIIAEEKPASVLIGSMRGRREFLWGLAARELGIPSVVYTVDCSIYPRLVFRPDLVLC
                     DDARQFEVATQEAKLPAHQVIQVSSHRRPPARSAAQTQTRAARPRLLLADTYYSGMVA
                     SSSPLLSAWAYQHVIEAARSLPGHDFFIKLHPVRERPDPVFHLSGQHHLHLWQRERNI
                     RSLNPPHNVTILAPEERLSDWLPGMDVLLNIQSYAGLEAFALRIPVIYVQPWDEEGLY
                     PRMNAMGVMQVAVDTPSLVRLIRLNLEDPGHCQRQLALQERYLDYFYWQGRPPCAEAA
                     ALPAAP"
     gene            complement(1211..4132)
                     /locus_tag="PLP58_16840"
     CDS             complement(1211..4132)
                     /locus_tag="PLP58_16840"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF012403.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HRJ10247.1"
                     /translation="MDNLCTCPSARRGMICEHSLAVALLGIRQAQEAAKPESKARISN
                     SRAEPALKEAALSVPGRYSVFLPDHLLAGGLRDGLAAYVKYEPDGEGETSRLAAWLDA
                     QGVKGRSAPLSLRGEILDQFLHALAGHPRVLCGKPSGGGIPIHVADAPVRLPLRATAE
                     GENVLFKIPAETGRPLLGACPERWWACQQTATLFALPASEGEVANLIRETALGSARRP
                     LLWLVARRVELDLVFQLELAGGVLENFHIAPVPCQYELALEGSLQVLEARLSAEFQGM
                     RWRLESDSSNGKSKNVFPIHDESSHCVFYVDDKMKNQRLFTRLRALGFSPMSGMDGEF
                     WRLTGGEKILAFHASELPRLHQEMKVLEGERWRTVTRGLARISPVIKPLEAPSDTPPG
                     GSGMDWLAMEFAYTAPDGFHLSRSEVLRLVRSGQRSVQGAAGKRYVLDLRGVEELEET
                     LKDVPVQITQGGARIHAFHAPYLTGDNLAERIGLIPENTITEQIGDLAAKLRPYQMLG
                     VRWMFSLVLKGAGGVLGDEMGLGKTVQSIAAIRALKHSSGDADLPVLIVCPKSLTGNW
                     RDEFKRFAPELKVAVCQGEKREDVLNSTSAYDVIITTYQLIVRDLKYHSTRKWRLVLL
                     DEASHIRNPDTEAAKALGKLESGARLALTGTPVENSVRDLWSIYHFALPGYLGSRENF
                     KERFEVPLAGGLDTPAAQAASERLKKLVRPYFIRRTKREVLRDLPEKIEQILWCDPTP
                     AQAEVYRRLLEEGRDEIKAARKRSGQSGAKMTMFTVLLRLRQVCCDLRLTGLGGETLA
                     GLSNEEISGKWGVLGERLDEIIESGGKVLIFSQFVQYLRLVRAELECQNLALCYLDGS
                     SQDREAQVKTFQADPQKRIFLISLKAGGYGLNLTAADHVILLDPWWNPAVEAQAIDRA
                     HRIGQERVVTATRLALRGTVEERILALQARKRGLVEAALDEESPLMIGLHAADLEGLL
                     D"
     gene            4370..5179
                     /locus_tag="PLP58_16845"
     CDS             4370..5179
                     /locus_tag="PLP58_16845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018291337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HRJ10248.1"
                     /translation="MRIVAITNQKGGVGKTTTAINLSACLAHLGHRVLLIDMDSQGNA
                     TSGLGLAKEEGGSIYAALVEGADPHEAIRATRLPNLSIIRAHQELAGCEIELAQRGNH
                     LTRLREVLDQIRASGHFDFVFLDCPPSLGVLMTSALAAADEMLVPLQCEYFGLEGLSS
                     IVQIVHQIRDCGANPNLLLEGIVMTMFDQRANLANQVVNDVKSNLAEMVYETIIPRTV
                     RLGEAPSFGKAIIEYEPSGRGAQAYRALAQEFLARRAAKPALSAMEPGLPR"
     gene            5325..6836
                     /locus_tag="PLP58_16850"
     CDS             5325..6836
                     /locus_tag="PLP58_16850"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013718.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vanadium-dependent haloperoxidase"
                     /protein_id="HRJ10249.1"
                     /translation="MSVFVRVLAVFLAVQFASPGVAQVLPGRHSAAFRAPYTPAAEIA
                     PRAVVRHRAMLGTPVQRWNEIAINASGLDHTPPRQGENRTYGEQLGPCRAARAMAIVH
                     LAIFEVVNCARATSESYLGRPQVGTVISLDAGIAQAAHDCLAALFPAQSSLFAGYLAE
                     DLALMRPGPAKIQGIALGQSIAAAVLALRENDGSDHDEPRVGIEHITANAPGRWRQDP
                     VSGHPLALGASWPQVTPFVLSSGSQFRLPPPPALDSLEYSMAYVEVKRLGGDGVTTPT
                     ERTEEESFIGVFWAYDGTPSLCAPPRLYNQIALAVAAEKRTTEMELARLLALVNLAMA
                     DAAIAAWDSKYHHDFWRPVTGIREGGPDAPDDGNEDTVGNPSFMPLGAPASNLTGPNF
                     TPPFPAYPSGHATFGGALFQVLRRFYGTDQIPFTFVSDEFNGVTLGNDGQARPLRPRT
                     FSSLSQAEEENARSRIYLGIHWDFDAYQGVKLGRQVGDYVFDHACKLKRPPRR"
     gene            complement(6852..7133)
                     /locus_tag="PLP58_16855"
     CDS             complement(6852..7133)
                     /locus_tag="PLP58_16855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009959690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ10250.1"
                     /translation="MSPAELASIKTPSGLAPEARALWLVKNGAWDDAHNAAQEIHTRT
                     GSWIHALLHVIEGDQWNADYWFAKAGKPSRGPADVDALWNEIAGSLPGQ"
     gene            complement(7171..8574)
                     /locus_tag="PLP58_16860"
     CDS             complement(7171..8574)
                     /locus_tag="PLP58_16860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007414600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HRJ10251.1"
                     /translation="MNQPSPAPASLAPWYKQLNPYHWFVFFVASAAWCFDCLDQQLFI
                     LARDNALKALDPEGDQAKLSGMAMSIFVAGWATGGLIFGAVGDRIGRAKTLALTVLIY
                     SVFTGLSSFAANADQFMIYRALTGLGVGGVFGLAVALVADSLPDTARAKALGLLQALS
                     AVGNVTAGMCAMAVSGMDPNVSWKYLFWIGSAPAFLCIFIMIRLREPEKWVAAKALSR
                     QDKSKAMGSYTSLFGDARWRTPALFGMLLCVSAVIGLWGIGFFSNKLVAPAIAAALAE
                     QNLSPEKVIAGKQWWAAANLIVQNVGAFFGMIVFSKAASRFGRKPVFAVSFIAAMAST
                     IFFYQCFKSTTDIWMSFVMGFCQLSIFAGFAIYLPELFPTRLRSTGTSFCYNVGRFIA
                     ASGPITLGVLASNLAKEAKAALPSIPDEAARKAAEAAAELVAFRNAGTWMSLIFLLGL
                     VALLFLPETKGRPMPED"
     gene            complement(8674..9717)
                     /gene="obgE"
                     /locus_tag="PLP58_16865"
     CDS             complement(8674..9717)
                     /gene="obgE"
                     /locus_tag="PLP58_16865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009962230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HRJ10252.1"
                     /translation="MFVDQIKVYARAGKGGDGNAHFHRGKFRPKGGPDGGDGGRGGDV
                     VLEADPSTDSLRPFFFNPKLVAKDGDKGGANLCTGRSAEAVVFKVPCGTLVSRIVESE
                     DAETGEVVESYEPFADLTQAGQRFVLCKGGKGGKGNVHFTTSTHQAPREFTPGEPGEE
                     GRFHFELRSIADAGFVGFPNAGKSTLLSKLSAARPKIANYPFTTLQPNIGVIHFGPNR
                     RGTLADIPGLIEGAHDNVGLGHDFLRHIMRCRILLFVVDAAGSEGRDPVHDLETVRTE
                     VSLYSKELARRSWHIIANKMDMPGAEENLERIKDRFKKIRVLPVSAETGEGLDKLRAF
                     LDQKIGQDHGIDF"
BASE COUNT         1876 a         3394 c         2815 g         1886 t
ORIGIN      
        1 cgccccggct gccggcgcgc acgcggcccg gccagttctg gtgcgccgac ccgcgccttg
       61 agtcaatcgt cgcccgggac gatcgcgaag gacgctgggt ggccggcccg cgcttcaccg
      121 aacctgccgc cggcgaggtc gaggcacttc gccaactcta cctggcatgg tgggagcgat
      181 ggtgggcgga ctggaaaaaa agccaccccg acgaggacgc cctcagcccg cgccatattc
      241 tcggcgatct cggacgctgg ttcagccgcg agcgttatcc tttgcatgcc tgccacctcc
      301 agcaggcccg ccggatcatc gccgaagaaa agcccgcgag cgtgctcatc ggctccatgc
      361 gcggacggag ggagtttttg tggggcctgg ccgcccgcga actgggcatc ccctctgtgg
      421 tatacacggt ggattgcagc atctacccgc gtctcgtttt ccggcccgac ctcgttctct
      481 gcgatgacgc gcgtcagttc gaagtcgcca cccaggaggc aaagctgcca gcgcatcaag
      541 tcatccaagt cagctcccac cgcagaccgc ccgcccgctc cgccgcgcag actcaaaccc
      601 gcgcggcccg cccccgcctg ctgctggcgg acacctatta ctccggcatg gtcgcatcca
      661 gttcgcctct gctcagcgcg tgggcctacc agcatgtcat tgaggccgcg cgctcactgc
      721 caggacatga ttttttcatc aaactgcatc ccgtccgcga acgtccggac ccggttttcc
      781 atctcagcgg ccagcaccat ctgcacctct ggcagcgcga gcgcaacatc cgcagtctga
      841 atccgccaca caatgtgacc atcctggccc cggaggagcg cctttccgac tggctgcccg
      901 gcatggacgt gctgctgaac atccagtcct atgccggcct ggaggctttt gccctgcgca
      961 ttccggtcat ttatgtccaa ccgtgggatg aggagggctt gtatccgcgc atgaacgcca
     1021 tgggagtcat gcaagtggcc gtggacacgc ccagcctcgt ccggctcatc cgcctcaatc
     1081 tcgaagaccc cggccattgc cagcgccaac tggccctgca agaacggtat ttggattact
     1141 tttactggca gggccgcccc ccatgcgccg aggctgccgc gctgcctgcg gctccttgat
     1201 ccaaccggcg ctaatccagc agtccctcca gatcggcggc atgcagaccg atcatcagcg
     1261 gtgactcttc atccaaagcc gcctcgacca accctcgttt cctggcctga agcgctagaa
     1321 tcctctcctc caccgttcca cgcagcgcaa ggcgggtggc agtgacgacc cgctcctgtc
     1381 cgattcggtg ggcgcggtca atcgcctgtg cctcgaccgc cggattccac cagggatcaa
     1441 gtaggatgac gtggtccgcc gccgtgaggt tgagaccata gccccctgct ttgagactga
     1501 tgagaaaaat gcgtttctgc ggatctgctt ggaaggtttt gacctgcgcc tcccgatcct
     1561 gactgcttcc gtccaggtag cagagggcca gattttgaca ttccaattca gcacgaacca
     1621 ggcgtaaata ttgcacgaac tgactgaata taagaacctt tccaccactt tcgatgattt
     1681 catcgagtcg ctcgccaaga actccccact tgccggaaat ttcctcgttt gatagacccg
     1741 ccaaagtctc gcctcccagc cctgtcagac gcaaatcaca acacacctgg cgcagtctca
     1801 acagcacagt gaacatggtc atcttcgccc cggactggcc ggagcgtttt cgcgccgcct
     1861 tgatctcatc ccgcccttcc tcaagcagtc ggcggtaaac ctccgcttgc gccggggtgg
     1921 ggtcacacca gagaatttgc tcaatcttct ccggcagatc ccggaggacc tctctctttg
     1981 tgcgacggat aaagtagggc cggaccagtt ttttgaggcg ctcgctcgct gcctgcgccg
     2041 ctggcgtgtc gagacctccc gccaatggaa cctcgaagcg ctctttgaaa ttctccctgc
     2101 tccccaaata accgggcaaa gcaaagtggt agatggacca taaatcccgg acactgtttt
     2161 ccacaggagt gcctgtgagg gccagccgag cgccagattc gagcttgccc agggcttttg
     2221 cggcctcggt atccgggttg cgaatgtggc tggcctcatc cagcaggacg agccgccatt
     2281 tccgagtgga atggtatttc aaatctctga caatgagttg ataagtcgtg attatcacat
     2341 cataggcgga agttgaattc agcacatctt cacgcttctc accctggcac accgccactt
     2401 tcagctccgg agcgaagcgc ttaaattcat cccgccagtt gcctgtcagt gacttcggac
     2461 aaacaatcag aacaggcagg tcagcgtctc ccgacgagtg tttgagggcg cggattgctg
     2521 cgatgctctg caccgttttg cccaagccca tctcatcccc caggactccg cccgccccct
     2581 tcaaaaccag agagaacatc cagcgaacac cgagcatttg atagggtcgg agtttggcag
     2641 ccagatcacc gatctgctca gtgattgtat tctcgggtat cagtccaatc ctctcagcca
     2701 aattgtctcc agtcaaatat ggcgcatgga aggcatggat gcgggctccg ccctgagtga
     2761 tctgcacagg cacatccttc agtgtttctt ccaattcctc cacgcccctc aaatccagca
     2821 cataacgctt cccggcggcc ccctgcaccg aacgctgacc ggagcgtacc agacgtagta
     2881 cttcggatct ggacaagtgg aacccgtctg gcgccgtgta ggcgaactcc atcgccagcc
     2941 aatccatgcc gctgccgcct ggaggcgtat cggacggcgc ttcgagcggt ttgatcacag
     3001 gggaaatccg cgccaaaccg cgtgtcaccg tccgccagcg ctctccctcc agcactttca
     3061 tttcctggtg caggcgcggc aactcagagg cgtgaaaggc caggattttt tctccacccg
     3121 taagccgcca gaattcccca tccatgccgg acatgggact gaatcccaga gctcgcaggc
     3181 gggtaaaaag ccgctgattt ttcattttgt catccacata aaaaacacaa tgagatgatt
     3241 catcatgaat cggaaacaca tttttcgatt tgccattcga tgaatcactc tccagccgcc
     3301 acctcatccc ctgaaactcc gcgctgagtc ttgcttccag cacttgcagg gaaccctcca
     3361 aagccagttc atactggcaa ggcacaggtg cgatatgaaa attttccagc accccgcccg
     3421 ccagttccaa ttgaaacacc aagtccaatt ccactcgccg cgccaccagc cagagcaaag
     3481 gccgccgagc acttcccagg gcggtttcgc ggatgagatt ggcgacttcc ccctccgaag
     3541 ctggaagagc aaacaaggtg gcggtctgct ggcaggccca ccagcgttcc ggacacgcgc
     3601 ccaggagcgg tctgcctgtc tctgccggaa tcttgaacag cacattctca ccttcggcag
     3661 tggctcgcag gggcaggcgc accggcgcgt cagccacatg gatggggatg ccaccgccgg
     3721 aaggcttgcc gcacagcaca cgcggatgcc cggccagcgc gtgcagaaat tgatcgagga
     3781 tctctccgcg caatgaaagg ggcgcgctcc ggcctttcac gccttgggca tccaaccagg
     3841 ccgctaacct gctggtctcc ccttccccat ccggctcata tttcacatat gcggccagtc
     3901 cgtccctcaa accaccggcc agcaaatgat ccggcaggaa cacgctatag cggcccggca
     3961 ccgacaaagc cgcctctttg agggcaggct ccgcgcgcga gtttgagatc cgggctttgc
     4021 tttcgggctt cgccgcttcc tgagcctgcc ggatgccgag caaggccaca gccagcgaat
     4081 gctcgcagat catgccgcgc cgggcggacg ggcaggtgca gaggttgtcc acgtccgtgc
     4141 gggagcggat gcggagtccg caggcgaact ttgttttgcc cccgcctgcc aggccgcgga
     4201 tgaactcgcc gtcggcgctt ttcagcgcgg ccagccccgc gtccgccagc ccgcgcgcgg
     4261 ctttcatcgc cgcccagccg ccgatctctc cgagccattt ctccgtgatt tccatttttt
     4321 cttggcgcgg cacatcgtta ccgttcacag tccgcggtca agacaactta tgcggatcgt
     4381 tgccatcacc aatcaaaagg gcggcgtggg gaaaaccacc acggccatca atctctccgc
     4441 gtgcctggcc cacctcggcc accgtgtgct gctcatagac atggactcgc agggcaacgc
     4501 caccagcggt ctcggactgg ccaaggagga gggaggcagc atttatgcgg ctttggtgga
     4561 gggggccgac ccgcatgagg ccatccgcgc cacccgcctg ccgaatctgt ccatcattcg
     4621 cgcccatcag gagctggccg gctgcgagat cgagctggcc cagcgcggca accacctcac
     4681 ccgcctgcgc gaggtgctcg accaaatccg cgcctcgggg catttcgatt tcgtgtttct
     4741 cgactgcccg ccttccctcg gagtgctgat gacctcggcg ctggccgccg ccgatgagat
     4801 gctcgtgccg ctgcaatgcg agtactttgg cctggaagga ctctccagca ttgttcaaat
     4861 cgtccatcaa atccgcgact gcggcgccaa tcccaacctg cttctggagg gcatcgtcat
     4921 gaccatgttc gaccagcgcg ctaacctggc caaccaggtg gtcaacgacg tgaaaagcaa
     4981 cctcgccgag atggtttacg agaccatcat cccgcgcacc gtccgccttg gggaggcccc
     5041 cagctttggc aaggccatca ttgagtatga accctccggg cgcggagccc aggcctaccg
     5101 cgcgctggcc caggaattcc tggcgcggcg ggccgccaaa cccgcgcttt ccgcgatgga
     5161 gccaggcctt ccccgttgag aaaaattcat ccgccgcctg ccaaaccgca agatttggct
     5221 cgtgaatcgt ctcgacaaac cggggcgctg gctttgaaat gccaatattc ccggctggat
     5281 gatcactgat gaaattccac gcgggttgat gatctggaat tgccatgagc gtcttcgtcc
     5341 gagttctcgc agtctttctg gcggtgcaat ttgcctcgcc gggagtggcc caggtgctgc
     5401 ctggcaggca cagcgccgct ttccgcgcgc cttacactcc cgcagctgag attgccccgc
     5461 gagccgtagt tcgccaccgg gccatgctgg gaacgccagt ccagcgctgg aacgagatcg
     5521 cgatcaatgc cagcggactc gaccacaccc ctccgcggca aggtgaaaac cggacctatg
     5581 gcgagcaact cggcccctgc cgcgcagcgc gcgccatggc aatcgtccat cttgccatct
     5641 tcgaagtggt caactgcgcg cgcgccacaa gtgagagcta tctggggcgc ccgcaggttg
     5701 gcaccgtcat ttcactggat gccggcatcg cccaggccgc ccatgactgc cttgcagcct
     5761 tgtttcccgc ccagagttcc ctttttgccg gctatctggc cgaagacctc gccctcatgc
     5821 gtcctgggcc ggccaagatc cagggcattg ctctcggcca gtccatcgct gccgctgtgc
     5881 ttgccttgcg tgaaaatgac ggctccgatc atgatgaacc ccgtgtcggg atcgaacaca
     5941 tcaccgcaaa cgcccccggc cgctggcgtc aggaccccgt cagcggccat cctctggcgc
     6001 ttggcgcgtc atggccgcag gtcacgccgt ttgttttgtc cagcggcagc cagttccgtc
     6061 tgcctccgcc gcccgcgctc gactctttgg agtactcgat ggcctatgtc gaggtgaagc
     6121 gtctcggcgg cgacggcgtc accacgccca cggagcgcac cgaggaggaa tccttcatcg
     6181 gcgttttctg ggcttacgac ggcacgccca gcctgtgcgc gccaccgcgc ctgtacaatc
     6241 agatcgccct cgctgtcgcc gctgaaaaac gaaccaccga aatggaactc gcccgcctgc
     6301 tggcgctggt caatcttgcc atggccgatg ccgccattgc cgcctgggac tccaaatacc
     6361 accatgattt ctggcgcccc gtcaccggca tccgcgaggg cggcccggac gcgcccgatg
     6421 atggcaacga ggacaccgtg ggcaatccct ccttcatgcc tctcggcgcg cccgccagca
     6481 acctcaccgg accgaatttc accccgccct tccccgctta cccgtcagga cacgccacct
     6541 ttggcggcgc gctgtttcag gtcttgcggc gtttttacgg caccgaccag attcccttca
     6601 cttttgtctc ggatgagttc aacggcgtga ccctcggcaa cgacggacaa gcgcgacctc
     6661 tgcgcccgcg caccttctcc agcctctccc aggctgagga ggaaaacgcg cgcagccgca
     6721 tctacctggg catccattgg gacttcgacg cttatcaggg tgtcaaactt ggccgccagg
     6781 tgggcgacta tgtttttgac catgcctgca agctgaagcg tccgccgcgc cgctgacgca
     6841 tcgggaaaac ctcactgacc cggcagcgat ccggcgattt cattccacag agcgtcaaca
     6901 tccgccggcc cccgcgatgg cttgcccgct ttggcaaacc agtagtcggc gttccattga
     6961 tccccctcga tgacatgcag cagcgcgtgg atccaggaac ctgtccgcgt gtgaatctcc
     7021 tgggcggcat tgtgcgcgtc atcccaggcg ccgtttttca ccagccacag cgcgcgggcc
     7081 tccggcgcaa ggccggaagg ggtcttgatg gaggcgagtt cggcagggga catgagggga
     7141 attcttttgg ctcgattccc agcatcggaa tcaatcttcc ggcatcggac gtcctttagt
     7201 ttcgggcaaa aacaggagcg cgacgaggcc cagcaggaaa atgaggctca tccatgtgcc
     7261 ggcattgcgg aaagcgacca attcagcggc tgcctccgcc gctttgcggg cggcctcatc
     7321 tggaatcgaa ggcagcgccg ccttggcctc ctttgccaga tttgaagcca gcacgccgag
     7381 tgtgatgggg ccgctggcgg caatgaaacg gcccacattg tagcaaaagc tcgtgccagt
     7441 gctgcgcagg cgggtgggga agagttcggg aagatagatc gcaaagccgg caaagatcga
     7501 gagctggcag aaacccatca caaagctcat ccaaatgtca gtggtgcttt tgaagcactg
     7561 gtagaaaaag atggtggagg ccatcgcggc gatgaaagac accgcgaaga cgggcttgcg
     7621 gccaaaacgg gacgcggcct tgctgaaaac tatcatgccg aaaaacgcgc ccacattctg
     7681 gacgatgagg ttcgcggccg cccaccattg cttcccggcg atgactttct caggcgatag
     7741 attttgctcc gcgagcgcgg cggcgatggc gggggcgaca agcttgttgc tgaagaatcc
     7801 aatcccccac aggccgatca ctgcggacac gcacaggagc atgccaaaca aggccggggt
     7861 gcgccagcga gcgtctccaa aaagactggt ataggatccc atcgccttcg atttatcctg
     7921 ccggctcagt gccttggcag caacccattt ctccggctcc ctgaggcgga tcatgatgaa
     7981 gatgcacaaa aaggcaggag cggagccgat ccagaataga tatttccaag acacattcgg
     8041 atccatgccg gaaaccgcca tcgcgcacat gccggcggtg acatttccca cggcggagag
     8101 cgcctgcagc aggcccagag ccttcgcacg cgctgtgtca ggcagggaat ccgcgaccag
     8161 cgccaccgca aggccaaaga ctccgcccac tccgagaccg gtcagcgcgc ggtaaatcat
     8221 gaactggtcc gcgttggcgg caaatgacga cagaccagtg aaaaccgagt aaatcagcac
     8281 cgtgagagcc agtgttttcg cgcgtccgat gcggtcgccc accgcgccga agatcagccc
     8341 gccggtggcc catccagcca cgaagatgct catcgccatg ccactgagtt ttgcctgatc
     8401 cccctcgggg tccaatgcct tgagagcgtt gtctcgcgct aggatgaaaa gctgctggtc
     8461 caggcagtcg aaacaccagg cggcgcttgc gacaaagaag acaaaccaat ggtacgggtt
     8521 tagttgcttg taccaggggg cgagggacgc gggagcaggg ctgggctggt tcatggagcg
     8581 gcgagcttga aacacttccg gcggcgtggc aatcacgaat tgccctttcc tcggcgtctc
     8641 ggcggcagcc cgtcgtttca gctccccgcc ggatcaaaaa tcaatcccgt gatcctgccc
     8701 gatcttctgg tccagaaagg cgcgcagctt gtccagcccc tcgcccgtct cggcagagac
     8761 gggcagcacg cggatttttt tgaagcggtc cttgatcctc tccaggttct cctccgcgcc
     8821 gggcatgtcc attttgttgg cgatgatgtg ccaggagcgt ctggccagct ccttggaata
     8881 aagcgaaacc tccgtgcgca ccgtctccag gtcatgcaca gggtcgcgcc cctcgctgcc
     8941 cgccgcgtcc accacaaaga gcaggatccg gcagcgcatg atgtggcgca ggaagtcatg
     9001 gcccaggccc acattgtcat gcgcgccctc gatcaggccg gggatgtccg ccagcgtgcc
     9061 gcgccggttc gggccgaagt gaatgacgcc gatgttcggc tggagcgtgg tgaacgggta
     9121 gttggcgatc ttcggccgcg ccgcgctcag cttcgacagc agcgtggatt tccccgcgtt
     9181 cgggaagccc acaaagcccg cgtcggcgat gctgcgcagc tcgaagtgga agcgcccctc
     9241 ctcgcccggc tcccccggtg tgaactcgcg cggcgcctgg tgcgtgctcg tggtgaaatg
     9301 cacattgccc ttgccgccct tgccgccctt gcacagcaca aagcgctgcc ccgcctgcgt
     9361 caggtccgcg aacggctcat agctttccac cacctccccg gtctcagcgt cctcgctctc
     9421 cacgatccgc gacaccagcg tcccgcacgg caccttgaag accaccgcct ccgcgctgcg
     9481 cccggtgcag aggttcgccc cgcctttgtc gccgtctttg gcgacgagct tcggattgaa
     9541 gaaaaacggc cgcaggctgt ccgtgctcgg atccgcctcc agcaccacgt caccgccgcg
     9601 cccgccgtcc ccgccgtcgg ggccgccttt ggggcggaac ttgccgcggt ggaagtgcgc
     9661 gttcccgtca ccacccttgc cggcgcgggc atagactttg atttgatcta caaacatgaa
     9721 ccctccacct agcccggcca gcccgtccgc acaagcgccg cgcgcgggga atccgctccc
     9781 ccgcgcctta cttccggttg ccgcgccccc gctccgcccg cttattccgg ccatgcctcc
     9841 gcgcgccaca ctttgccgca tccttctgct tgtcctcgcg ggcttccacc tccccgcgct
     9901 ccgcgcgcag accttgcagc tcactcccgc agacatgctc cgccgcgccg tgccagggca
     9961 gcccgcgccc g
//
LOCUS       DAOMYJ010000192         9949 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Prosthecobacter sp. isolate AMR_MDS_5629
            NODE_2266_length_9949_cov_24.106125, whole genome shotgun sequence.
ACCESSION   DAOMYJ010000192 DAOMYJ010000000
VERSION     DAOMYJ010000192.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867850
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prosthecobacter sp. (wastewater metagenome)
  ORGANISM  Prosthecobacter sp.
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Verrucomicrobiaceae; Prosthecobacter.
REFERENCE   1  (bases 1 to 9949)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9949)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.30
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:34:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,742
            CDSs (total)                      :: 4,684
            Genes (coding)                    :: 4,676
            CDSs (with protein)               :: 4,676
            Genes (RNA)                       :: 58
            tRNAs                             :: 55
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9949
                     /organism="Prosthecobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2266_length_9949_cov_24.106125"
                     /isolate="AMR_MDS_5629"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1965333"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(17..1570)
                     /locus_tag="PLP58_16870"
     CDS             complement(17..1570)
                     /locus_tag="PLP58_16870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009965152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HRJ10253.1"
                     /translation="MDRDEHPANTAPTFLDTAAAGIVTPGSPFPPPPLKPKKAPSRKP
                     ARAIGVKTFVLDTNVLLHDPACIQRFAEHHVCVPVDVLSELDRFKNEMTERGANAREV
                     HRALMKVFNAPDASVTEGVRTNGGGNLRIVVYDPHMAADVRSVLEFQRIFPGQDKMDH
                     RILACALWMRERLDTPVILVSKDLNMQLKARAVGIECEDYLHDKVEPREVANFDIARV
                     QVSGHELQRFASSGRITLPGSRLHDIAPNDYVLLTAGEKSSMPARMSAQGELLRLHHT
                     PESIKVGQGRAIKPMNLGQTCLLDALLDPEISLVTCYGQAGTGKTLLAVAAGLSQVFA
                     RVYHGVTISRPIVAMGQTVGFLPGTLQEKMRPWLQPVYDALDLLMRPVDNSQGPQRKK
                     NRFLPDKPAPAEAAAPYDPLIQQGVIEIEALCYIRGRSIPDRFFVLDEAQQLTPLEAK
                     TIVTRMSRGSKLVLVGDPAQIDNPYVDSRSNGLVYTRQRMRGQPFAAHVPLSKGERSP
                     LAEAAARLL"
     gene            complement(1842..2189)
                     /locus_tag="PLP58_16875"
     CDS             complement(1842..2189)
                     /locus_tag="PLP58_16875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009958904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HRJ10254.1"
                     /translation="MKKIEAIIKPFKLEDVKEALSDVGVEGMTVIEVKGFGRQKGHTE
                     IYRGSEYTVDFLPKVKIEVVVEDSRSDTVVEAIVKAANTGKIGDGKVFVSEVLEAVRI
                     RTSERGADAIAGS"
     gene            complement(2406..3431)
                     /locus_tag="PLP58_16880"
     CDS             complement(2406..3431)
                     /locus_tag="PLP58_16880"
                     /inference="COORDINATES: protein motif:HMM:NF024808.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0019808 - polyamine binding [Evidence
                     IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015846 - polyamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     substrate-binding protein"
                     /protein_id="HRJ10255.1"
                     /translation="MKALLCALAVSLLALAQVRAAGVLHVYTWADYLSPEAVESFEKA
                     HDCRVIVDTFDSNESLHARLKAGAKGYDVIFPTSYMVRLLQQEDLLLPLDHAALPNLR
                     HLDREVLDKVHDREMRHAVPYTLGYAVIAVRSDRLKDAAPTWAMFEREIPGRRATLLD
                     DMRETLGAALKHLGHSLNSRDEAELAAARDVVIRWKRGIAKFDNEGYKGGLDSGEFHL
                     VHGYSGDLFQVAQENRRVQILIPREGVSIGCDEMVIPKTSPQPALAMQFINHLLDPEV
                     AAENMEWLGYLCPNTAALKLVSPDFLQNPAVTIPAEVKAKSEVMDDLGSDLAKWTKVW
                     DEIKAAR"
     gene            complement(3428..4201)
                     /locus_tag="PLP58_16885"
     CDS             complement(3428..4201)
                     /locus_tag="PLP58_16885"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRJ10256.1"
                     /translation="MKRSRLPALATLLVLVFLHAPLVVLVGNSFNASRFGGEWEGFTW
                     QWYERLWQADDIWDSLWLTLKLALGSTLLATVLGTLSAWALHHFSSRLQRLHRGLMLL
                     PLMMPEVLMGMALLSLFTALGVQTGMMTLWAAHVTFCVSYVAMVVLGRLRGFDPHLLE
                     AARDLGASPWQAARRVVLPLLLPGILAGALLAFTLSLDDFVISFFVSGPGSTTLPLRI
                     ASMMKTSRSLPVINALSTLLISATFLFATLAFRLNRARS"
     gene            complement(4297..5166)
                     /locus_tag="PLP58_16890"
     CDS             complement(4297..5166)
                     /locus_tag="PLP58_16890"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRJ10257.1"
                     /translation="MKAGVQFSKKPAEWLLTAPPLLWLGVFFVIPTVLILGTALRPAD
                     LHGGLAEGWSLEAVRAMTDAVYPPIWWRTLWMSAATTLLCLLIALPVGWHLARLTRRW
                     QGIVLLLLTLPFLTNFLIRVFAWKSLLHPEGALTRLCLALGWMSEEQQLLYNAGSVLA
                     VLVYVHLPFAILPVYAAAEKFDFSLLDAARDLGAGALRGFVSVFIPGVRGGIGAGALM
                     VFTGCLGQYVVPQIVGGTEDEMIGSKIAQRAFSDRNLPEAAALTGVLLLAMLVPLLML
                     AWRRKGATTNGHE"
     gene            5312..5542
                     /locus_tag="PLP58_16895"
     CDS             5312..5542
                     /locus_tag="PLP58_16895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ10258.1"
                     /translation="MKQYRPTIEQLKKRYSADMARRKMQARRDAARDPNAKRVRIPDN
                     SEPWLPHPVRSVADAMWISDQITADMRQWSLL"
     gene            5527..6225
                     /locus_tag="PLP58_16900"
     CDS             5527..6225
                     /locus_tag="PLP58_16900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ10259.1"
                     /translation="MEPSLISADTFIHALSAKHRVVLLGGMAIISHGLSRKTKDVDIW
                     LDPMNTTAAWAAALKECLDVFSGVYLWSLADRCVLAHEDVAGEIEENGVLRVSEFDRD
                     IDVFRRPNELGIESFEEVWQRSVKVLEGGVRLPDPLDLYISKANTGREHDWQDQLFLE
                     SLVKARFKERLPVCDLAEATTMLDRFLDPECLQYARTNPHPEVRALALKYLREFEAEG
                     DPYSRDILAAGGWE"
     gene            complement(6186..6404)
                     /locus_tag="PLP58_16905"
     CDS             complement(6186..6404)
                     /locus_tag="PLP58_16905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ10260.1"
                     /translation="MGEIWREQNDPLPAPFEGVEIKDALNETVVRQGVTAEEARQVLK
                     DKGRLSSAEMVRLRGRYSHPPAAKISRE"
     gene            6496..9717
                     /locus_tag="PLP58_16910"
     CDS             6496..9717
                     /locus_tag="PLP58_16910"
                     /inference="COORDINATES: protein motif:HMM:NF025253.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0098024 - virus tail, fiber [Evidence
                     IEA];
                     GO_process: GO:0019062 - virion attachment to host cell
                     [Evidence IEA];
                     GO_process: GO:0046718 - viral entry into host cell
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber domain-containing protein"
                     /protein_id="HRJ10261.1"
                     /translation="MKTYLTLIGVLLFLAAFTAHAQTTAFTYQGHLMDGGASANGSYD
                     LQFTLKNALSGGSTVGTPQAIAPVSVVNGIFTVTLDFGGAVFDGSDRWVEMGVRPFGS
                     AVPYTVLAPRQKLTSAPYAVRAANAGNAANAANLTGTLTAGNVPANLITGAMLANNTI
                     TATQIQNGAVGSAQLANNAVGADQIASGAVTLSKIAATSASGYLGQGALSGLTGGTAT
                     ATVIFAQPFGSTPPVVIQQPGWTLGAVSTTGFSATTPFAPVTVDSTGNVGRDTSLAVV
                     NGRPAISYWDLSNVDLKYAIAPSADGTGTWTTVTVDSAGNVGEHTSLAVVYGRPAISY
                     RDGNNGYLKYAIAPSADGTGTWTTLTVDSVGSDTSLAVVNGRPAISYWDFTNGDLKYA
                     IAPSADGTGTWTTLTVDSAGSVGQYTSLAVVNGRPAISYRDFTNGDLKYAIAPSADGT
                     GAWTTLTVDSTGNMGEHTSLAVVNGRPAISYRDVTNHDLKYAIAPSADGTGAWTTLTV
                     DSAGLVGSDTSLAVVNGRPAISYWDFSNGDLKYAIAPSADGTGGPWTTFPVDSAGDVG
                     RYTSLAVVHGRPAISYYDETNGDLKYAQLPDGLWTAGDALATAPVLAAGVQAGAVGAD
                     QLAASAVDASKIAEGAVDDSKLAPAISATKLGSGLVDNTEFNFLDGVTSGVQEQLGGK
                     VSKAGDTMTGGLTVGGNGDLIVSGPTARFALNTGGYTDVAATIKGRAGDSSILDLQSS
                     TGADVFFVHDAGNTTIAGNTTIAGSVIIAASSGTGRLALNTGGYSNVAATIKGRAGDS
                     SILDLQSSGGTDVFYVNDAGNTTIAGNTFISGNVGIGTGAPTAKLDVNGTARMTGPLT
                     LQTTTSDASATARLSLIANGAHEWRLQSVTGFGQPIPGSGPTASFQLINMTSAVTALT
                     ALQDGKVGLGRIPTANRLEVEGDASKTTASGWAANSDRRIKEDIQPVMNALDTLGKVR
                     LVDFRYTDDYRAAHPGIADQRYLNVVAQEFAEVFPDHVKSSGEKLPDGSEILQVDTYP
                     LTIYSAAAVQELHAKLKAKEDEIAEMKARLERVEKLLEKGGTP"
BASE COUNT         1856 a         3130 c         3279 g         1684 t
ORIGIN      
        1 agggcaaagc agggcgtcag agcaggcgcg cggcggcctc ggccaggggg ctgcgctcgc
       61 ccttgctgag ggggacgtgg gcggcgaagg gctggcccct catgcgctgg cgggtataga
      121 cgaggccgtt gctgcggctg tccacatagg ggttgtctat ctgggcgggg tcgccgacga
      181 ggacgagctt cgagccgcgt gacatgcggg tgacgatggt cttggcctcg aggggtgtga
      241 gctgctgggc ctcatcaagc acgaagaagc ggtcggggat ggagcgtccg cggatgtagc
      301 agagggcctc gatctcgatg acgccctgct ggatgagggg gtcgtaaggc gcggcggcct
      361 cggcgggggc gggtttgtcg ggcaggaagc ggtttttctt gcgctggggt ccctggctgt
      421 tgtccacggg gcgcatgagg aggtccagcg cgtcatagac gggctggagc caggggcgca
      481 ttttttcctg cagggtgccg ggcaggaagc cgacggtctg ccccatggcg acgatggggc
      541 ggctgatggt gactccatga tagacgcggg cgaagacctg ggaaagaccg gcggcaacgg
      601 cgaggagggt cttgcccgtg ccggcctggc cgtagcaggt gacgaggctg atctcggggt
      661 cgagcagggc gtcgagcagg caggtctggc cgaggttcat gggcttgatg gcgcggccct
      721 ggccgacttt gatgctctcc ggggtgtggt ggaggcgcaa cagctcgccc tgggcgctca
      781 tgcgcgcggg catggaggat ttctccccgg cggtgagcag gacgtagtcg ttgggggcga
      841 tgtcgtgcag gcgggagccg ggcagggtga tgcgcccgct gctggcgaag cgctggagct
      901 cgtggccgct gacctggacg cgggcgatgt cgaaattcgc cacctcgcgc ggctcgactt
      961 tgtcgtgcag gtagtcctcg cactcgatgc cgacggcacg ggctttgagc tgcatgttca
     1021 ggtccttgct gacgagaatg acgggggtgt cgagccgctc gcgcatccag agcgcgcagg
     1081 ccaggatgcg gtggtccatt ttgtcctggc cgggaaagat gcgctggaat tccaggacgc
     1141 tgcgcacatc ggcggccatg tgcgggtcat agacgacgat gcgcaggttg ccgccgccgt
     1201 tggtgcgcac gccctcggtg acggatgcgt cgggggcatt gaagaccttc atcagcgcgc
     1261 gatgcacctc gcgcgcgttg gcgccgcgct cggtcatctc gtttttgaag cggtccagct
     1321 cgctgagcac gtccacggga acgcagacgt ggtgctcggc gaagcgctgg atgcaggcgg
     1381 ggtcgtgaag caggacgttg gtgtcgagga cgaaggtttt aacgccgatg gcgcgggcgg
     1441 gtttgcggga gggggctttt ttcggtttca aaggcggtgg cggaaaggga ctgccgggcg
     1501 tgacgatgcc tgcggcggcg gtgtcgagga atgtgggggc ggtgttggcg gggtgttcgt
     1561 cacggtccat gggagtacgg gttcgatggg tttgcaatcc ggtctctgag ggcgggtctt
     1621 cttcctgtcg cgttcgtggc gccaggcccg cctgtccgca tgattgattt ttgcgaaatc
     1681 acggattttc cagaggctgt cagaggattg gaatttcgac aagagtttta ttcacaaaac
     1741 ttaactttct cgaagtagtt cgctgagcaa ggccgcgaat gccggcacaa aaaaagcccg
     1801 ccggttgatg gaccggcggg ctgaattttt tgaaacgcgg ttcaagatcc ggcgatggcg
     1861 tccgctccgc gctcactggt gcggatgcgc acggcctcaa gcacttcgct gacgaaaact
     1921 ttgccgtcgc cgattttgcc ggtgttggcg gctttgacga tggcctcgac cacggtgtcg
     1981 ctgcgggagt cctcaacaac gacctcgatt ttgactttgg gcaggaaatc aacggtgtac
     2041 tccgaaccac ggtagatttc cgtgtggcct ttttgacggc cgaagccttt gacctcgatg
     2101 acggtcattc cttcgacgcc cacatcagag agggcttctt tcacgtcttc gagtttgaac
     2161 ggcttgatga tcgcttcgat ttttttcatg gctgacgcaa ttaggtgtgt ggtttggcgg
     2221 attgcaacga cgagcaagca tcaatcatga attttgccgg tcggattatg caaataccat
     2281 caaagcggaa tccatgccaa atttcagatt catttttttg aaaaatttca gccggaggcg
     2341 gatcgccggg accggcatgc ggattccccg cctttccgaa ccgcgagaaa aggccggttt
     2401 tacagtcacc gcgcggcttt gatttcatcc cagaccttcg tccacttcgc gaggtcggag
     2461 ccgaggtcgt ccatgacctc gcttttggcc ttcacttcgg cggggatggt gacggcggga
     2521 ttttgcagga agtccgggct gacgagtttc agcgcggctg tgtttgggca gaggtagccg
     2581 agccattcca tgttttcggc ggccacttcg gggtcgagca ggtggttgat gaattgcatc
     2641 gccagcgcgg gctgcggaga ggtcttcggg atgaccattt catcgcagcc aatgctcacg
     2701 ccttcccgcg ggatgaggat ctgcacgcgg cggttctcct gggccacttg aaagagatcg
     2761 ccgctgtagc cgtggacgag gtggaactca ccggagtcga ggccgccttt gtagccttcg
     2821 ttgtcgaatt tggcgattcc gcgcttccag cggatgacca catcccgcgc ggcggccagc
     2881 tcggcttcgt cgcgtgaatt caggctgtgg ccgaggtgtt tcaaagccgc gccgagcgtc
     2941 tcgcgcatgt catcgagcag ggtggcgcgt cggcccggga tctcgcgctc aaacatggcc
     3001 caggtggggg cggcgtcttt gagacggtcc gaacgcacgg cgatgacggc gtaaccgagc
     3061 gtgtagggca cggcatggcg catctccctg tcatgcacct tgtcgagcac ctcgcggtcc
     3121 agatgccgca ggttcggcaa agcggcgtgg tcgagcggca gcagcaggtc ctcctgctgg
     3181 agcagccgca ccatgtagct ggtgggaaag atcacatcgt aacccttggc ccccgctttg
     3241 aggcgcgcgt gcaggctttc gttcgagtcg aaggtgtcca caatgacacg gcagtcgtgg
     3301 gctttttcaa aagactcgac ggcctccggg ctcagataat ccgcccaggt atagacatgc
     3361 agcacgccag ccgcgcggac ttgggcgagg gcgagcaaag agacggcaag cgcgcaaaga
     3421 agggccttca tgaccgcgca cgattgagcc ggaaagccag cgtggcgaac aaaaacgtgg
     3481 cggagatgag cagggtgctg agggcgttga tgaccggcag gctgcggctc gtcttcatca
     3541 tgctggcgat gcgcagcggc agcgtggtgc tgccggggcc ggagacgaaa aaggaaatga
     3601 cgaagtcatc cagcgagagc gtgaaggcca gcagcgcgcc cgcgaggatg ccgggcagca
     3661 gcagcggcag caccacacgg cgcgcggcct gccaggggct ggcgccgaga tcgcgcgcgg
     3721 cctccagcag atgcgggtca aagccgcgca accgcccgag cacgaccatc gccacataac
     3781 tcacacaaaa ggtcacatgc gcggcccaga gcgtcatcat gccagtctgc acgccgagcg
     3841 cggtgaagag cgagagcagc gccatgccca tgagcacctc cggcatcatg agcggcagca
     3901 gcatgaggcc gcggtgaagc ctttgcaggc ggctggagaa atgatgcagc gcccatgccg
     3961 aaagcgtgcc cagcaccgtg gcgagcaggg tggagccgag cgccagcttg agcgtcagcc
     4021 agagcgagtc ccagatatca tccgcctgcc agagacgctc ataccattgc caggtgaagc
     4081 cctcccactc cccgccgaag cgcgaggcat tgaaggaatt ccccaccagc accaccagcg
     4141 gcgcgtgcag aaacacgagc accagcagcg tggcgagggc gggcaggcgg gagcgtttca
     4201 tggggcggat tttaaccacg aatggacacg aattcgcacg catggctgcg gacattcggt
     4261 gccacacagc tcttcagaat ggatgaaaat tcgtgtctat tcgtgtccat tcgtggttgc
     4321 tcccttccgc cgccaagcca gcatcagcag cggcaccagc atggccagca gcagcacgcc
     4381 cgtgagcgcc gccgcctcgg gcaggttgcg gtcgctgaag gcgcgctggg cgattttgct
     4441 gccgatcatc tcatcctccg tgccgcccac aatctgcggc accacatatt gcccgagaca
     4501 gcctgtgaag accatcaaag cccccgcccc tataccacca cgcacgccag ggatgaagac
     4561 ggagacaaag ccccgtaaag ctcccgcgcc cagatcgcgc gcggcatcga gcaggctgaa
     4621 gtcgaacttc tccgccgccg catacaccgg caggatggcg aagggcagat gcacataaac
     4681 cagcacggcc agcaccgacc cggcattgta gagcagttgc tgctcctcgc tcatccagcc
     4741 cagcgcgagg cacaggcggg tgagcgcgcc ttcggggtgc agcagggatt tccaggcgaa
     4801 gacgcggatg aggaagttcg tgagaaacgg cagcgtcagc agcagcagca cgatgccctg
     4861 ccagcgccgc gtgagccgcg ccagatgcca gccaacgggc agggcgatga gcaggcacag
     4921 cagcgtcgtg gccgcgctca tccacagcgt gcgccaccag atgggcggat acaccgcgtc
     4981 cgtcatcgcg cgcaccgcct ccaggctcca gccctccgcc aaacccccat gcaggtccgc
     5041 cggacgcaga gcggtgccca ggatcaggac ggtggggatg acgaaaaaga cgcccagcca
     5101 gagcagcggc ggcgcggtga gcagccactc ggcgggcttt ttcgaaaatt ggactccggc
     5161 tttcatgcgc tgcggcagtc aacccgaagg cacctccagg atcaaggctg agatgaaccg
     5221 ggaatggcag ggcaacggcc agccctcacg cagcgccgag tggcgatgcc ttgttgcatc
     5281 cagcagtgag gagagatagg ctgccgccag gatgaagcag taccgcccca ccatcgagca
     5341 gctcaaaaag cgctactccg ccgacatggc acggcggaag atgcaggcgc ggcgggatgc
     5401 ggcgcgtgat ccgaatgcaa agcgggtgcg gattcccgac aactccgagc cctggctgcc
     5461 gcatcctgtt cgctccgttg cggatgcgat gtggatttct gatcaaatca ccgcagacat
     5521 gcgccaatgg agccttcttt gatcagtgcg gacaccttca ttcacgctct gtccgcgaag
     5581 catcgcgtgg tgttgctggg cggcatggcc atcatcagtc atggactgag ccggaagacc
     5641 aaggatgtgg acatctggct ggatccgatg aacacgacgg cggcttgggc agcagccctg
     5701 aaggaatgtc tggatgtttt ttcaggagtc tatctctggt cgctggcgga tcgctgtgtg
     5761 cttgcgcatg aggatgtggc tggcgagatc gaagaaaacg gcgtgctgcg tgtctcggaa
     5821 ttcgaccgtg acattgacgt ctttcgccgc ccgaatgaac tcgggatcga gtcatttgag
     5881 gaggtctggc agcgtagtgt caaggtactg gagggcggtg tgcgcctgcc tgatccactg
     5941 gacctgtata tctcgaaagc caacacaggc cgcgagcatg actggcagga tcagcttttc
     6001 ctggaatccc tggtcaaagc ccgcttcaag gagcgcctgc cggtttgtga tctcgcggag
     6061 gccacgacga tgctcgaccg ctttctggac ccggagtgcc tgcaatacgc gaggaccaat
     6121 ccgcaccccg aggtccgcgc gctggcgctg aaatacctgc gcgagttcga ggccgagggc
     6181 gatccttact cccgcgatat tttggcggcg ggcgggtggg agtagcgccc ccgcagccgc
     6241 accatctcgg cgctgctcag cctgcctttg tccttgagca cctgccgcgc ctcctccgct
     6301 gtcacgccct ggcgcaccac ggtctcattt agcgcgtctt tgatctcgac tccctcgaat
     6361 ggagcaggca gcgggtcatt ttgctctcgc caaatctctc ccaaaatgca atcctcgctg
     6421 ccggttcagg ttataccggg cgctctgcgt gttgagtagt gacctggatg cagacgttat
     6481 tcagccgccg acaccatgaa aacatacctc accctcatcg gtgtgctgct cttcctggca
     6541 gcgttcaccg cgcacgccca aaccaccgcc ttcacctacc aggggcatct catggacggc
     6601 ggcgcctcgg ccaacgggag ctatgacctc cagttcacgc tgaagaacgc gctcagcggg
     6661 ggctccaccg tcggcacgcc gcaggccatc gcgccggtga gcgtggtgaa tggcatcttc
     6721 actgtcacac tggacttcgg cggtgcggtg ttcgatggca gcgaccgctg ggtggagatg
     6781 ggcgtgcggc ccttcggcag cgcggtgcct tataccgtgc tcgcgccgcg gcagaagctc
     6841 accagcgccc cctacgcggt gcgggctgcg aatgcaggca atgccgccaa cgccgcgaac
     6901 ctcaccggca ccctcaccgc cgggaatgtt cccgccaatc tcatcaccgg cgccatgctg
     6961 gcgaacaaca ccatcaccgc cacgcagatc cagaatggcg cggtgggcag cgcgcagttg
     7021 gcgaacaatg ccgtgggtgc cgaccagatt gccagcggcg cggtcacgct gtcgaagatc
     7081 gccgccacca gcgcctcggg ctacctgggc cagggagcgc tcagcggact caccggcggc
     7141 acggccaccg ccaccgtcat ctttgcgcag ccctttggca gcacgccacc ggtggtcatc
     7201 cagcagccgg gctggacgct cggtgcggtg agtaccaccg gcttcagcgc caccacgccc
     7261 tttgctcctg tcaccgtgga cagcacgggc aacgtgggaa gggacacctc gctggcggtg
     7321 gtcaatggcc ggcccgccat cagttactgg gatttgagca acgtcgacct gaaatacgcc
     7381 attgccccca gcgccgacgg caccggaacc tggaccaccg tcaccgtgga cagcgcgggc
     7441 aacgtgggag aacacacctc gctggcggtg gtctatggcc ggcccgccat cagctaccgg
     7501 gatggcaaca acggctacct gaaatacgcc atcgcaccca gcgccgacgg caccggaacc
     7561 tggaccaccc tcaccgtgga cagcgtggga tccgacacct cgctggcggt ggtcaatggc
     7621 cggcccgcca tcagttactg ggatttcacc aacggcgacc tgaaatacgc catcgccccc
     7681 agcgccgatg gcaccggcac ctggaccacc ctcaccgtgg acagcgcggg gagcgtggga
     7741 cagtacacct cgctggcggt ggtcaatggc cggcccgcca tcagttaccg ggatttcacc
     7801 aacggcgacc tgaaatacgc catcgccccc agcgccgacg gcaccggcgc ctggaccacc
     7861 ctcaccgtgg acagcacggg aaacatggga gaacacacct cgctggcggt ggtcaatggc
     7921 cggcccgcca tcagctaccg ggatgtcacc aaccacgacc tgaaatacgc catcgccccc
     7981 agcgccgatg gcaccggcgc ctggactacc ctcaccgtgg acagcgcggg gttggtggga
     8041 tccgacacct cgctggcggt ggtcaatggc cggcccgcca tcagttactg ggattttagc
     8101 aacggcgacc tgaaatacgc catcgccccc agcgctgatg gcaccggcgg cccttggacc
     8161 accttccccg tggacagcgc gggggatgtg ggacgctaca cctcgctggc ggtggtccat
     8221 ggccggcccg ccatcagtta ctatgatgaa accaacggcg acctcaaata cgcccagctc
     8281 ccggatggcc tgtggacggc gggagatgcc ctggccactg ccccggtgct ggccgcaggg
     8341 gtgcaggccg gcgcggtggg cgccgaccag ctcgccgcca gcgccgtgga tgccagcaag
     8401 atcgccgaag gtgccgtgga cgacagcaag ctggccccgg ccatcagcgc caccaagctg
     8461 ggctctgggc tggtggacaa caccgagttc aacttcctcg atggcgtcac ctcgggggtt
     8521 caggagcagc ttggcggcaa agtgagcaaa gccggtgaca ccatgacggg cggcctcaca
     8581 gtcggcggca atggcgacct gatcgtctcc ggtcccaccg cccgctttgc tctgaacacc
     8641 ggcggttaca ccgacgtcgc cgccaccatc aaaggccgtg cgggtgacag cagcatcctt
     8701 gatctccaga gctcaacggg agccgatgtc ttctttgtcc atgacgctgg caacacgacc
     8761 atcgctggca acacgaccat cgctggcagc gtcatcatcg ccgcaagcag cggcaccggc
     8821 cgtctggccc tgaacaccgg cggttactcc aacgtcgccg ccaccatcaa aggccgtgcg
     8881 ggtgacagca gcatccttga tctccagagc tccggcggca ccgatgtctt ctatgtcaat
     8941 gacgctggca acacgaccat cgctggcaac accttcatca gtggcaacgt cggcatcggc
     9001 accggcgctc ccaccgccaa gctcgacgtg aacggcacgg ccaggatgac cggcccgctc
     9061 accctgcaaa ccaccacgag cgacgcaagc gccaccgcgc ggctgagcct catcgccaac
     9121 ggcgcgcacg agtggcggct gcagagcgtg accggctttg gccagcccat ccccggctcg
     9181 gggcccaccg ccagcttcca gctcatcaac atgacctccg ccgtcaccgc cctcaccgcg
     9241 ctgcaggatg gcaaagtcgg cctcggccgc attcccacgg ccaaccggct cgaagtcgag
     9301 ggcgacgcgt ccaagaccac cgccagcggc tgggcggcga acagcgaccg gcggatcaag
     9361 gaggacatcc agcccgtgat gaatgcgctc gacacgctgg gcaaggtgcg gctggtggac
     9421 ttccgctaca ccgatgacta ccgcgccgcg caccccggca tcgccgacca gcgctacctc
     9481 aacgtggtgg cgcaggaatt cgccgaggtc ttccccgatc acgtgaaaag cagcggcgag
     9541 aaactgccgg atggcagcga gatcctccag gtggacacct acccgctgac gatctactcc
     9601 gccgccgcgg tgcaggagct gcatgcgaag ctcaaggcga aggaggatga gatcgccgag
     9661 atgaaggccc ggctggagcg ggtggaaaag ctgctggaga aaggagggac cccttgagac
     9721 tgagtggaca gcacctcggg ctgcggtccg gaccgaatca gggcggcttt cctctcctga
     9781 gagacggcaa atcaactccg gggagtttcc gtcaggggtg gcacccccgg cggctgccca
     9841 tgccaccccc cgcgccaggg ggtggcaccc ccggcggctg cccatgccac cccccgcgtc
     9901 agggggtggc acccccggcg gctgcccatg ccaccccccg cgtcagggg
//
LOCUS       DAOMYJ010000193         9803 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Prosthecobacter sp. isolate AMR_MDS_5629
            NODE_2308_length_9803_cov_19.696861, whole genome shotgun sequence.
ACCESSION   DAOMYJ010000193 DAOMYJ010000000
VERSION     DAOMYJ010000193.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867850
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prosthecobacter sp. (wastewater metagenome)
  ORGANISM  Prosthecobacter sp.
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Verrucomicrobiaceae; Prosthecobacter.
REFERENCE   1  (bases 1 to 9803)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9803)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.30
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:34:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,742
            CDSs (total)                      :: 4,684
            Genes (coding)                    :: 4,676
            CDSs (with protein)               :: 4,676
            Genes (RNA)                       :: 58
            tRNAs                             :: 55
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9803
                     /organism="Prosthecobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2308_length_9803_cov_19.696861"
                     /isolate="AMR_MDS_5629"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1965333"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..612
                     /locus_tag="PLP58_16915"
     CDS             <1..612
                     /locus_tag="PLP58_16915"
                     /inference="COORDINATES: protein motif:HMM:NF013226.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prenyltransferase"
                     /protein_id="HRJ10262.1"
                     /translation="RAAWLMLGVAALLSLPLISARGWPVIAIGVPSLYFCFGYTGGPW
                     PLAYRGLGELFVVLFFGFVAVAGSAFVQSGQWHAGAILAGFQIGCLSTILIAINNLRD
                     MDEDRSTGKLTLAARFGPRFARWEITLLILLPHAAGAAWCLAGKAQAFVLPLLAAPAG
                     LVILRRVWTTPPGPDYNRLLALGGAQLLAFALLFCLGLDGADG"
     gene            688..1554
                     /locus_tag="PLP58_16920"
     CDS             688..1554
                     /locus_tag="PLP58_16920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009963966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ThuA domain-containing protein"
                     /protein_id="HRJ10263.1"
                     /translation="MKKLLAPVLLLGLAAVFYLANAAESPVKPLRVLIVAGGCCHDYE
                     TQQALLKEGIEKRLHAKVDVAYNPDKTTKAVFEIYKAKDWGKDFDVVIHDECSADVTD
                     PAYVGNILAAHKAGMPAVNLHCAMHSYRWGNFREPVQPGADNAAWYEFLGLQSTGHGP
                     QEPIDITYTDAGHPVTKGLENWTTIKEELYNNIQILTGKPLASGRQIQPPKAKKGQPL
                     PPDAKPVTAEAVVAWTNEYGPGKTRVFSTTLGHNNDTVADPRYLDLVTRGLLWAVGKL
                     AEDGTPAAGYAK"
     gene            complement(1642..2121)
                     /locus_tag="PLP58_16925"
     CDS             complement(1642..2121)
                     /locus_tag="PLP58_16925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009962186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fur family transcriptional regulator"
                     /protein_id="HRJ10264.1"
                     /translation="MSRHHHHHHAGCRRPEPERLLSESMLRAREAGLRRTRALEDVLS
                     ILIHACQPLTLADIAESDRLRSGADKATVYRLLMKLEERSLIRRLGLHDRSAYYILNL
                     PGEHHDYLICNDCGRIEKLDITCPVQSLEKQIAKKSGFKKLYHELEFFGQCPDCADA"
     gene            complement(2131..3072)
                     /locus_tag="PLP58_16930"
     CDS             complement(2131..3072)
                     /locus_tag="PLP58_16930"
                     /inference="COORDINATES: protein motif:HMM:NF013866.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HRJ10265.1"
                     /translation="MKHHLCLHLALLLLIANLHAAPRTWTSSDGKSSFQGELVEFTDT
                     EVKIKHSGNFQIFKLPVERLSTEDQAHVRGLLRERRRDDGLKNGPYAAQITGQMVKGV
                     SKRGLNYQISGNPKWDGAQRYPLLIWLHGAGQSGDDNESQMGGAPKPMLTEDSQKKHP
                     CFFLAPQCPSRDIGWKNEVAENLVALIADLVENLPVDESRIYLTGSSMGGSGTWSIAA
                     RYPQIFAAAVPLCGGGDPKNAEVLKTIPFWVFHGDQDDQVPVDRSRVMVEAVKKAGGE
                     HMNYTELEGAGHLITATVYPRPELHEWIFAQRKKAAE"
     gene            complement(3184..4305)
                     /locus_tag="PLP58_16935"
     CDS             complement(3184..4305)
                     /locus_tag="PLP58_16935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009957911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HRJ10266.1"
                     /translation="MNLTRTAYGTWSGGKFMHFGEMLDDERYIGMIRQAFDKGVRTFV
                     TADVYGAGRADSMLGQALQGLPREEYSLVGIVGHDFYGGLRAGAKGYPRFTEPSLRQP
                     AEYAGYLRMAAEESLKRCGVGKFDCLMLHNPDTTGYTSEAVWDGLSALRDAGLTDRLG
                     IAPGPANGFTLDMIDCFERFGDRMEWAMIILNPFEPWPGQHVLPAAQKHGVDILARVV
                     DYGGIFHDDVKPGHPFRDGDHRAFRPAGWVEHAHEKLEKIRPIAEKHGLTMIQLASLW
                     NLAQAPVKSVVPTLIQEAGADAKPIEAKLDELAALPVENPLSAEEVELIRQIGDNTGC
                     MMLKGASQRHEGQEPRADEWPMRSELLALSARWGLGSAW"
     gene            complement(4496..5122)
                     /locus_tag="PLP58_16940"
     CDS             complement(4496..5122)
                     /locus_tag="PLP58_16940"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF013509.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HRJ10267.1"
                     /translation="MDKVSYFIGSQIGGNIANNFKQQGIEVDLENFLQAVRDKFEGKP
                     SKFTEQELTAAMESFEKIMQSKQADLEKEQMAKAGEVKAAGSKFLAENGKREGVVTTA
                     SGLQYEVLKKGDGAKPVATDKVNVHYHGTLLNGKVFDSSVERNMPITFGVQEVIKGWT
                     EALQLMSVGSKFKLYIPSSLAYGDTGAGPDIGPGETLIFEVELLGIEK"
     gene            complement(5212..5568)
                     /locus_tag="PLP58_16945"
     CDS             complement(5212..5568)
                     /locus_tag="PLP58_16945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ10268.1"
                     /translation="MCEEACVVCMEEPPLSPLNMWGKNNMLVSEFLPSSALRRSFGLR
                     RRKTLNKMTTTMPSMAAISTKDRNPMMTSMTADRLRKNPFHANSNMRSCPLPIANQAR
                     FLRLTATVPSPNLAPS"
     gene            5775..5942
                     /locus_tag="PLP58_16950"
     CDS             5775..5942
                     /locus_tag="PLP58_16950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ10269.1"
                     /translation="MKTTLLLLVPVLVMLASCRGTGGPATRAGRGIDHAVYKVGSGVR
                     KAGQKIENAAR"
     gene            complement(6024..8165)
                     /locus_tag="PLP58_16955"
     CDS             complement(6024..8165)
                     /locus_tag="PLP58_16955"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018290773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004654 - polyribonucleotide
                     nucleotidyltransferase activity [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HRJ10270.1"
                     /translation="MAIHKQTVQLGAGTLEIETGKLAKLADGAVTVRLGETIVIVTAV
                     SATKIKEGLDFFPLSVEYKEKASAAGKFPGGYFKREGRPTEKEILTCRMTDRPLRPLF
                     PKGYFYETQVVAILLSADGENDSDILAMNGASAALCVSDIPFAGPIGAVRVGRVGGQF
                     VINPTHSQREESDLDLVYVGSKTDVIMIEGAANELPEADFVAALRFAQESIQPIIQAQ
                     EALAAAAGKEKRVVPLMLAKEDLLEVAYAVAGDRIEGALYQPSKVARGKAVGALKDEV
                     EAAIKAKYPEATAFEISQAFDYLQKKAFRISILDKLSRCDGRAIDQIRPLSGEVSVLP
                     RTHGSAIFARGETQALAIATLAPADEAQEMDTYAGGPDSKRFILHYNFPPFSVGECGR
                     FGGQNRREIGHGALAERSIEPVIPPKETFPYAIRVSSEVMESNGSTSMASVCSGVMAL
                     MDAGVPLKRPVAGISVGLVTEFEGEQMKRYLTMLDILGSEDHFGDMDFKLCGTSEGVT
                     GYQLDLKLPGIPLSILEEAVTKAKNGRTEVLAAMAQGISEVKPLSPHAPRIEIIKINP
                     DKIGELIGPGGKNIKAIQAESGAEISIEDDGTVYVYATRREGLERAIEMINGVSQEIE
                     PGKTYTGKIVSTKEFGAFMNLGGKKDGLIHISELADFRVNKVEDVVKVGDIVTAKCLG
                     IDEKGRVKMSRKAAMKEKDEAAKSASGEDVG"
     gene            complement(8346..8603)
                     /gene="rpsO"
                     /locus_tag="PLP58_16960"
     CDS             complement(8346..8603)
                     /gene="rpsO"
                     /locus_tag="PLP58_16960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009962082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HRJ10271.1"
                     /translation="MSEATISPSTFQLHEKDTGSSDVQVAILTERINHLTEHLASHSK
                     DHSSRRGLLKLVARRRKLLDYLKLTANERYQKLLKSLSLRR"
     gene            complement(8674..9672)
                     /locus_tag="PLP58_16965"
     CDS             complement(8674..9672)
                     /locus_tag="PLP58_16965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ10272.1"
                     /translation="MTAEQTAKLNPPQASNQGNAGLHGAFWTKIMLFWMRLLPAWLCL
                     ALARPAAFLVYLLAGPQRRAVMANVGALQPGFGQLRLWWAGYQVFLQFALAYLDRLWH
                     MHFRQEVAWDIPDKPHFDEMRGHPGGVLIFTIHSGNYDVGASLFAEKFGRTLHMVRVP
                     EQTAELQKLREAELRGVEREHPHLRVHYNEADSHLGLELCRALMEGEAVAVQGDRVVT
                     GVSPVEMRHGGLCFLIPRGPLVLAEISRAPAWPIFLQRLSRMRYRILVGKAFSEAGEK
                     VRADEVGRRWLPVLHAHVRQHWDQWFVFESLVARHPGKAVPDLEQPGKSPCTKSSS"
BASE COUNT         1634 a         2896 c         3319 g         1954 t
ORIGIN      
        1 cgtgcggcct ggctcatgct cggcgtggcg gcgctgctgt ccctgccgct catttccgcg
       61 cgcggctggc ccgtcatcgc catcggcgtc ccgtcgctgt atttctgctt tggctacacg
      121 ggcggtccct ggccgctggc ttaccggggg ctcggggagc tgtttgtcgt gttgtttttc
      181 ggcttcgtcg cggtcgccgg ttccgcattt gtccagagcg gccaatggca tgccggagcc
      241 atccttgcag gcttccaaat cggctgcctc tcgaccattt tgatcgccat caacaacctg
      301 cgcgacatgg acgaggaccg ctccacgggg aagctcacgc tggccgcgcg cttcggcccg
      361 cgcttcgcac ggtgggagat cactctgctc atcctgctgc ctcatgccgc gggtgcggcg
      421 tggtgtctgg cgggaaaggc gcaggctttc gttttgccgc tgctggcggc acccgcaggc
      481 ttggtgatcc tgcgccgcgt ttggacaact cctcccggcc ccgactacaa ccggctgctc
      541 gcgctcggcg gcgcccagct actggccttc gcgctgcttt tctgcctggg cctggacgga
      601 gcggacggtt gacgcgcccg ctgccaaacc acgggcaata ttcgcgcctg cgccgcgttt
      661 gcttggcgtc tgttgaaccc aaccaccatg aaaaaactgc tcgcccccgt cctgctgctc
      721 ggcctcgccg ctgtctttta ccttgccaat gccgcggagt ccccggtcaa gccgctgcgc
      781 gtgctcatcg tcgccggcgg atgctgccac gattatgaga cccagcaggc cctgctcaag
      841 gaaggcatcg aaaagcgcct ccacgccaag gtggacgtgg cctacaaccc cgacaaaacc
      901 accaaggccg tcttcgaaat ctacaaggcc aaagactggg gcaaggactt cgacgtggtc
      961 attcacgatg aatgctccgc cgatgtcacc gaccccgcct atgtgggcaa catcctcgcc
     1021 gcgcacaagg ccggcatgcc cgccgtcaat ctgcactgcg ccatgcacag ctaccgctgg
     1081 ggcaacttcc gcgagcctgt gcagcccggc gcggacaacg ccgcgtggta tgaattcctc
     1141 ggcctgcaat ccaccggcca cggcccccag gagccgatag acatcaccta cacagacgcg
     1201 ggccaccccg tcaccaaggg gctggaaaac tggaccacca tcaaagagga gctttacaac
     1261 aacatccaaa tcctgaccgg caaacccctg gccagcggcc gccagatcca gccgccgaaa
     1321 gcgaaaaaag gccagcccct gccgcccgac gccaagcccg tcaccgccga ggccgtcgtg
     1381 gcctggacca atgagtacgg gccgggaaaa acccgcgtct tcagcaccac gctcggccac
     1441 aacaatgaca cagtggccga tccccgttat ctcgatcttg tcacacgcgg actgctttgg
     1501 gccgtcggca agctcgcgga agacggcacg cctgccgcgg gatatgcgaa ataggccgct
     1561 gaatgcggcg gtctccgcca ttgagtgccg cgctgttttc gtgaacgcga aagaacttcc
     1621 accgagactc ttccgtcgtg ctcaggcatc cgcgcagtcg gggcactggc cgaagaattc
     1681 gagctcgtgg taaagctttt tgaagccgga ttttttggcg atctgctttt ccagggactg
     1741 cactgggcag gtgatgtcga gcttctcgat gcggccgcag tcgttgcaaa tgaggtagtc
     1801 gtggtgctcg ccgggcaggt tgaggatgta gtaggcggag cggtcgtgca ggccgagcct
     1861 gcggatgaga gagcgctcct ccagcttcat gagcaggcga tagaccgtgg ccttgtccgc
     1921 gccgctgcgc agccggtcgg attcggcgat gtcggccagg gtcaggggct ggcaggcgtg
     1981 gatgaggatg gaaagcacgt cctcaagcgc gcgggtgcgg cgcagcccgg cttcgcgcgc
     2041 gcgcagcatg gactcggaga gcagcctttc cggttcggga cgcctgcatc cggcgtgatg
     2101 atgatggtgg tggcgggaca ttgaggcggc tcactcggcg gcttttttcc tctgcgcgaa
     2161 gatccattcg tgcagttcgg gccgcggata aacggtggcg gtgatcaggt ggcccgcgcc
     2221 ctcgagttcg gtgtagttca tgtgctcgcc gccggctttt ttcaccgcct cgaccatgac
     2281 gcgggagcgg tccacgggca cctggtcgtc ctggtcgccg tggaagaccc agaaggggat
     2341 ggttttgagg acttcggcgt ttttggggtc gccgccgcca cagaggggaa cggctgcggc
     2401 gaagatttgc gggtatcttg ccgcgatgct ccaggtgccg ctgccaccca tgctggaacc
     2461 ggtgaggtag atgcggcttt cgtcaacggg gagattttcg acgaggtcgg cgatgagggc
     2521 gacgaggttt tcggcgactt cgtttttcca gccgatgtcg cgcgaagggc actgcggggc
     2581 gaggaagaaa cagggatgtt ttttctggct gtcctcggtc agcatgggct tgggcgcgcc
     2641 gcccatctgg gattcgttgt catctccgct ctggcccgcg ccgtggagcc agatgagcag
     2701 ggggtagcgc tgggcgccgt cccatttggg attgccgctg atctggtagt tgaggccgcg
     2761 cttggagacg cctttgacca tctggccggt gatctgggcg gcataggggc cgtttttgag
     2821 gccgtcgtcc cgccggcgct cgcgcaacag gccgcgcaca tgggcttggt cttccgtgga
     2881 gagccgctcg accggcagtt tgaagatttg gaaattgccg ctgtgcttga tcttgacctc
     2941 ggtgtcggtg aactcgacca gttcgccttg aaagctggat ttgccgtcac tggaagtcca
     3001 tgtgcgcggc gccgcgtgaa gattggcaat gagaagcagc agcgccaggt ggaggcagag
     3061 atggtgtttc atgaccgcga atgctagcag cccagtcagg gcgaggcaag acggtcttgg
     3121 tgtgttcgcg tcgggcaacc gggagggttg ccgccacttt ggagcatgat tgaagcggcg
     3181 cgctcaccag gcgctgccga gaccccagcg cgcggagagg gcgagcagct cggagcgcat
     3241 cggccactcg tcggcgcgcg gctcctggcc ttcgtggcgc tggctggcgc ctttgagcat
     3301 catgcagccg gtgttgtcgc cgatctggcg gatgagttcc acttcttcgg cactgagcgg
     3361 gttttccacc ggcagggcgg cgagctcgtc gagcttggcc tcaatgggct tggcatccgc
     3421 gccagcctcc tggatgaggg tggggacaac gcttttcacg ggtgcctgcg ccagattcca
     3481 caggctggcg agctggatca tggtaaggcc gtgcttttcg gcgatggggc ggattttctc
     3541 cagtttctca tgggcatgct ccacccagcc cgcggggcgg aaggcgcggt ggtcgccgtc
     3601 gcggaaaggg tggccgggct tcacgtcatc atggaaaatg ccgccgtagt ccacgacacg
     3661 ggcgagaatg tccacgccgt gcttttgcgc cgcgggcagc acatgctggc cgggccaggg
     3721 ttcaaaggga ttgaggatga tcattgccca ctccatgcgg tcgccaaagc gttcgaagca
     3781 gtcaatcatg tcgagcgtga agccattggc cgggccgggg gcgatgccga ggcggtcggt
     3841 gaggcccgcg tcgcgcaggg cgctgaggcc gtcccacacc gcctcgctcg tgtagcctgt
     3901 ggtgtcggga ttgtgcagca tgaggcagtc gaacttcccc acaccacagc gtttcaggga
     3961 ttcctccgcg gccatgcgca ggtagcccgc atactccgca ggctggcgca ggctcggctc
     4021 ggtgaagcgc gggtagccct tggcgccggc gcgcaggccg ccgtaaaaat catggccgac
     4081 gatgccgacg aggctgtact cctcgcgcgg cagtccttgc agcgcctggc cgagcatgga
     4141 atcggcgcgg cccgcgccat agacatccgc ggtgacaaag gtgcgcacgc ctttgtcaaa
     4201 tgcctggcgg atcatgccaa tgtagcgctc gtcgtcgagc atctcgccga agtgcatgaa
     4261 tttgccgccg ctccaggtgc cgtaggccgt gcgtgtgagg ttcatgatga aaaagtgtgg
     4321 gggggttggg tgacggggaa acctttaaaa agggatgcgc ctgatgcaaa caccagctcg
     4381 cccggaacca atccatgcgt ggaaatgacc agaatcgggg atgatgccag cgccttccgg
     4441 ggataaaaaa acgaccgccc ggagcgggcg gtcgtcggag gggaaggcac ggggcttact
     4501 tttcaatgcc gagcagctct acttcgaaga tgagagtctc tccggggccg atgtcggggc
     4561 cggcgccggt gtcaccgtat gcgaggctgg aggggatgta gagtttgaat ttggagccga
     4621 cgctcatgag ttggagggcc tcggtccagc ctttgatgac ctcctgcaca ccgaaggtga
     4681 ttggcatgtt gcgctccaca ctgctgtcaa agaccttgcc gttgaggagg gttccgtggt
     4741 agtggacgtt cactttgtcc gtggccacgg gtttggcgcc gtcacctttt ttgaggactt
     4801 catactgcag gccgctggca gtcgtcacaa cgccttcgcg cttgccgttt tcagcgagga
     4861 atttggagcc ggcggccttg acctccccgg ctttggccat ctgctccttc tccaggtccg
     4921 cctgtttgga ctgcatgatc ttttcaaagg actccattgc cgcggtcaac tcctgctcgg
     4981 tgaatttgga gggcttgccc tcgaacttgt cacgcacggc ttggaggaag ttttccaggt
     5041 caacctcgat gccttgctgc ttgaagttgt tggcgatgtt gccgccgatc tggctgccga
     5101 tgaaatagct gactttgtcc atgggagcgg gagccgcggc ggcggcttcg gtttgagctg
     5161 agacgggggc tgcgaacgcg agcgcggcgg cagcgagtgc gagggtgggt ttcatgaggg
     5221 cgcgagattc ggagagggaa ccgtggcggt caaccggaga aaacgggcct gattggcaat
     5281 gggcagcggg catgaacgca tattagagtt tgcgtgaaac gggtttttgc gcagcctgtc
     5341 ggccgtcatg gaagtcatca tcggatttct gtctttagtg ctgattgccg ccatgcttgg
     5401 catcgtggtt gtcatcttgt tcagcgtctt tctccgccgc aagccgaagg accggcgtag
     5461 agcggatgag gggagaaact ccgacaccag catgttgttc tttccccaca tgttcagcgg
     5521 ggacagcgga ggctcctcca tgcacaccac gcatgcttct tcgcacatgc cggacagcag
     5581 ccatgactcc ggctcgtcga gttggttcag tggtgatagt ggcggaagct ttggcgggga
     5641 cttcggaggc ggtggtgggg attgcggcgg agggggaggc ggaggggact gatggcgcac
     5701 gcagtctgga acgcggatgc ctttcggcga tcccgcggct tgaaaagccg cgcctggcgg
     5761 gtcataacac gggcatgaaa accaccctgc ttctgcttgt cccagtgctc gtcatgctcg
     5821 cgtcatgccg cggaacggga ggtccggcca cccgggcggg gcgcggcatt gaccacgcgg
     5881 tttacaaagt gggaagcggt gtgaggaagg ccggccaaaa gatcgaaaac gcggcccgct
     5941 gaacgcggcg cgtgaagcca ggggcagaaa aaaacggcag ccggtcaccc ggctgccgtg
     6001 attcgcgcga cttaccggag ggatcagcca acgtcttcac ccgaggcgga tttggcggcc
     6061 tcgtcttttt ctttcatcgc ggctttgcgg ctcatcttca cgcggccttt ctcgtcaatg
     6121 ccgaggcatt tggcggtgac gatgtcgccg actttgacga cgtcctcgac tttgttgacg
     6181 cggaagtcgg ccaactcgct gatgtggata aggccgtcct ttttgccgcc gaggttcatg
     6241 aaggcgccga actccttggt gctgacgatc ttgccggtgt aggtcttgcc gggttcgatt
     6301 tcctgggaga cgccgttgat catctcaatg gcgcgctcca ggccctccct gcgggtggca
     6361 taaacataga cggtgccgtc gtcctcgatg ctgatctcgg cgccggactc ggcctggatg
     6421 gccttgatgt tcttcccgcc ggggccgatg agctcgccga tcttgtcggg gttgatcttg
     6481 atgatctcga tgcgcggtgc gtgcgggctg aggggcttca cctcgctgat gccctgggcc
     6541 atggcggcga ggacttcagt gcggccgttt ttggccttgg tgacggcctc ctccaggatg
     6601 ctgagcggaa tgccggggag cttgaggtcg agctggtagc cggtgacgcc ctcgctggtg
     6661 ccgcagagtt tgaagtccat gtcgccgaag tggtcctcgg agccaaggat gtcgagcatg
     6721 gtgaggtagc gcttcatttg ctcgccctcg aactcggtga cgaggccgac ggaaataccg
     6781 gcgaccgggc gtttcagggg cacgccggcg tccatgaggg ccatgacgcc ggagcagacg
     6841 gaggccatgg aggtggagcc gttggattcc atgacttcgc tgctgacgcg gatggcgtag
     6901 gggaaggttt ccttcggcgg gatgaccggc tcgatggagc gctcggcgag ggcgccgtgg
     6961 ccgatctcgc ggcggttctg cccgccgaag cgtccgcatt caccgacgga gaaaggcggg
     7021 aagttgtaat gcaggatgaa gcgcttgctg tccgggccgc cggcgtatgt gtccatctcc
     7081 tgagcctcgt cggccggggc cagggtggcg atggcgagcg cctgggtctc gccgcgggcg
     7141 aagatggcgg aaccatgcgt gcgcgggagc acggagacct cgccggagag cgggcggatt
     7201 tggtcaatgg cgcggccatc gcagcgactg agcttgtcga ggatggagat gcggaaggct
     7261 ttcttctgaa ggtagtcaaa ggcctggctg atctcgaagg cggtggcttc gggatatttg
     7321 gctttgatag cagcctcgac ctcgtctttc aaggcgccga cggctttgcc gcgggcgacc
     7381 ttggagggct ggtagagggc gccttcgatg cggtctccag cgacggcgta ggcgacttcc
     7441 aggaggtcct ccttggcgag catgagcggg acgacgcgct tttccttgcc agcggcggcg
     7501 gcgagcgcct cctgggcctg gatgatgggc tggatgctct cctgggcaaa gcggagcgcg
     7561 gcgacgaagt cggcttcggg cagctcgttg gccgcgcctt cgatcatgat gacgtcggtc
     7621 ttgctgccga cataaacgag gtcaagatcg ctctcctcac gctggctgtg ggtggggttg
     7681 atgacgaact ggccgccgac gcgtccgaca cgcacggcac cgatggggcc ggcgaaggga
     7741 atgtcggaga cgcagagagc ggcggaggcg ccgttcatcg cgaggatgtc ggagtcgttt
     7801 tcaccgtccg cgctgagcag gatggcgacg acctgggtct cgtagaagta acctttcggg
     7861 aagagcgggc gcaggggccg gtccgtcatg cggcaggtga ggatttcctt ttccgtgggg
     7921 cggccttcgc gcttgaagta accgccgggg aatttgccgg cggcggaggc cttttccttg
     7981 tactccacgg agagggggaa gaagtcgaga ccttccttga tcttcgtggc ggagacggcg
     8041 gtgacgatga caatggtttc gccgagccgc acggtgacgg ctccgtcggc gagcttggcg
     8101 agcttgccgg tttcgatttc gagggttcca gcaccgagct ggacggtttg tttgtggatg
     8161 gccatatggc tgcttttgtg tgtttttggt ttgggaaaat gagccgcgcg gattgggagc
     8221 gcgcgtcaaa tgcggtttgt aagaattctc aggacgatca tgcgtcctgg agtggaatgc
     8281 ccgtgctgcg cgtcaaaact cgaagggcga tgccagcggc atcaccctcc ggaaaacgca
     8341 ttctgtcagc ggcgcaggct gaggctcttg aggagcttct gataccgctc gttggcggtc
     8401 agcttgaggt agtccagcag cttgcggcgg cgcgcgacca gcttgagcag gccgcggcgg
     8461 gaggaatgat ccttcgaatg gctcgcgagg tgctcggtga ggtggttgat gcgctccgtc
     8521 aagatcgcga cctgcacgtc ggaacttccg gtgtcctttt catgcagttg gaaagtgctg
     8581 gggctgatgg tggcttcgct catggtctgg tgtcggtgcc cgcgtcctgc gggcattggg
     8641 ttagtgtctt tggtttagga ggggcggaaa gaatcacgaa gacgactttg tgcaaggaga
     8701 ttttccgggt tgttcgaggt cagggacggc cttgccggga tgccttgcca ccagtgattc
     8761 gaagacaaac cactggtccc agtgctgccg cacatgcgcg tgcagcacag gcagccaccg
     8821 ccgccccacc tcgtccgcgc gcactttttc acccgcctcg ctgaaggctt tgcccaccag
     8881 gatgcggtac ctcatgcggc tcagtcgctg caaaaaaatc ggccatgcgg gcgcgcggga
     8941 aatttccgcc agcacgagcg gcccgcgcgg gatgaggaaa cacagcccgc cgtgcctcat
     9001 ctccaccggg gagaccccgg tgaccacgcg gtctccctgc accgccaccg cctcgccctc
     9061 catgagcgcc cggcacagtt ccaggccgag gtggctgtcc gcctcgttgt aatgcacgcg
     9121 caggtgcggg tgctcgcgct ccacaccgcg cagttccgct tctctgagct tctgcagttc
     9181 ggcggtctgc tcgggcacgc gcaccatgtg cagggtgcgt ccgaattttt ccgcgaacag
     9241 ggacgcgccc acgtcgtaat tgccgctgtg aatggtgaaa atgagcacgc cgcctggatg
     9301 cccccgcatt tcgtcgaaat ggggcttgtc tggaatgtcc caggccacct cctgccggaa
     9361 gtgcatgtgc cagagacggt cgaggtaggc cagtgcgaac tgcaaaaaca cctggtaacc
     9421 cgcccaccac aacctgagct gtccgaaacc gggctgcaaa gcgccgacgt tcgccatcac
     9481 cgcgcgccgt tgcggcccgg ccagcagata gacgagaaat gcagcgggcc gcgccagtgc
     9541 cagacacagc caggcgggca gcaggcgcat ccagaaaagc atgattttcg tccaaaacgc
     9601 cccgtgcagc cccgcgttcc cctggttgga ggcttgcggc ggattcaatt tggcggtttg
     9661 ttcggcggtc atcagcgtca tggtcaggcc cgccgcccgt cccccgtggt cccggtctgg
     9721 cgcgccctcc ctgtttttta aaactttgcg catccccgcc tgcgagtcaa aacatgaaca
     9781 ccgccgcccc cgcccccgcc ccc
//
LOCUS       DAOMYJ010000194         9717 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Prosthecobacter sp. isolate AMR_MDS_5629
            NODE_2331_length_9717_cov_18.158559, whole genome shotgun sequence.
ACCESSION   DAOMYJ010000194 DAOMYJ010000000
VERSION     DAOMYJ010000194.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867850
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prosthecobacter sp. (wastewater metagenome)
  ORGANISM  Prosthecobacter sp.
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Verrucomicrobiaceae; Prosthecobacter.
REFERENCE   1  (bases 1 to 9717)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9717)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.30
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:34:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,742
            CDSs (total)                      :: 4,684
            Genes (coding)                    :: 4,676
            CDSs (with protein)               :: 4,676
            Genes (RNA)                       :: 58
            tRNAs                             :: 55
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9717
                     /organism="Prosthecobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2331_length_9717_cov_18.158559"
                     /isolate="AMR_MDS_5629"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1965333"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..223
                     /locus_tag="PLP58_16970"
     CDS             <1..223
                     /locus_tag="PLP58_16970"
                     /inference="COORDINATES: protein motif:HMM:NF019040.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF1501 domain-containing protein"
                     /protein_id="HRJ10273.1"
                     /translation="MSVLMAGAGIRGGRVVGGTDARAEYPKDRPIYPEDITKTVYYAM
                     GVSDLEAHDRLGRAYNLLEDGRALTEMFG"
     gene            complement(220..804)
                     /locus_tag="PLP58_16975"
     CDS             complement(220..804)
                     /locus_tag="PLP58_16975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ10274.1"
                     /translation="MDLGSLNSLLREIQRKAPGRRILIFGSSSLLASFPDVPPEQIGV
                     AVTLDADFFLDPDDEAVRLDLHDTLGKNHEYHAATGHYGDFVDLRLSESFPDGWRARL
                     VPMPGFEDVLALEPVDMAVTKVAATARSRWTRRMGRGGVDRGMKDIRTIAALIRGGRM
                     DHAVLQKRLQGMDYEPALIVECAQVMREIEALLA"
     gene            complement(786..1145)
                     /locus_tag="PLP58_16980"
     CDS             complement(786..1145)
                     /locus_tag="PLP58_16980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ10275.1"
                     /translation="MPAKTAFAYLDSRTERPGDEVSADVFRAIAGKIEADPSLLRIPL
                     ENIDRWLARGHSAVSRLEQWRAVLLSAMSSNDGLRSLLALLRDSSAEAVFFKEFSPFP
                     GVLSSEELKRFRWTSAH"
     gene            1235..3565
                     /locus_tag="PLP58_16985"
     CDS             1235..3565
                     /locus_tag="PLP58_16985"
                     /inference="COORDINATES: protein motif:HMM:NF024753.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HRJ10276.1"
                     /translation="MKFVTAALLLLIVITLHAEDWPQFRGVNGSGVSVSRGLPGEFSA
                     EKNVLWKARLGDGVGSAIIKGGVVFATGMAGEGRVALHALDAASGAVKWRSEFETGTL
                     PRITPPNSHAASTPAADGERVYVHFSTIGLLAFDAATGKEVWRHSMPRPAYLMDWGAA
                     ASPVVHEGKVIFCQDDDLAPFLVAVDAATGREVWKTPRPDMLAGYALPVLCEAGGRVD
                     LVVAGSGKMKGYDPATGRELWTCNTLLRTIMTSPVVHEGVVYLAVQSYGDSTRTLKHA
                     LLEWLDTNQDGILAREETPKEFHERFDASDKNKNGLIDPEEIDTAFQSPDNMAAGGNI
                     IQAIKGGGSGDVTKTHVLWNLDHKTPSNIASPLLYHGRLHIVKGGGMASCYDAKDGAQ
                     LWDRVRLGNFGDYFASPVAADGKIFIAGKNGFVVVLEDGAELKVLGKNDMGEEIIATP
                     SIADGRLYIRTRENLFCIASGGSKAVLADSAAVAAPVEMTLSPVVSGRVWNGYTGNAM
                     GQESWSEAELEQRLEQLKKLGYTTVAVPGKIAPFAPIRVDGDTGGRKAFRGARTFENP
                     DVAAITTRFREKAAKLGFEVVTADPVAVSVLPGNEFPDEKTLAALVTPLCGEGVAARM
                     WLGFQAVDKAARLIAENDPALGIPAPDMITRHLNAGEAPPAWLVEVKGLYATAMNEMY
                     RANTRAREGSRSFTLYQAKRMESAFHFLNAIEAMHKAAADATIRAESLTAAQESLYNA
                     LNAWSDAARDSSDRGAIALLNEHGYRVLKKQAAQGE"
     gene            3700..4218
                     /locus_tag="PLP58_16990"
     CDS             3700..4218
                     /locus_tag="PLP58_16990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ10277.1"
                     /translation="MSDTPATSCCSRLDLAFANLIARLWVALRLVMAGVDKFRAGDGA
                     EATFSTANYETKTKLISDLMTQNSFLPAFLPASAIDMYAHSIGYLLIAVGLWVAVGLL
                     SEFALVAAGLTFLSLGFGLAALPDDSELTINIGIGIMITFMALMTNKCAWFSLDGLFG
                     RHRAKKVVVTEA"
     gene            4249..5730
                     /locus_tag="PLP58_16995"
     CDS             4249..5730
                     /locus_tag="PLP58_16995"
                     /inference="COORDINATES: protein motif:HMM:NF013567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HRJ10278.1"
                     /translation="MSTSSATPSTGLSRFMDRRSFLRTSAVSGAGLYIATSKSAIAQG
                     EQAGRTIKCALIGCGAQGDRLRVASSQIKTGIQWVAVADIWKYNRNPVARRMEFENKH
                     QVEGPVAQYEEVEEMLDKHPEIEAVFIATPDHLHAPFSRLCLEKGKSVYCEKMMSNTI
                     EGARDMVKAGRENNGIFQIGHQRHSNPRYIHLREKILKKGLLGRVTHCYGQWNRGVAA
                     SQPLGIPKNQTIPAEMLEKYGFGSMEEFRNWRWFAKYGGGPISDLGAHQIDMYNWMYE
                     TTPVSLFAAGGVDYYDGTPGPDGEPKAKFELPDNVMCLYEYQTKEGVMRAYYQVLTTT
                     GSQGYYEKHMGVAGSAIISESPTYNQVYAEPGSDWSQYAMGDDPILVKAPDAVKNKFW
                     EHPRSWAKPVPKSYNVTGVAKAVADARESKALDPWEIPVILEVYPHTPHVANFIEAVQ
                     MKKPAHLTCNVMDAFKSCVTVLKAYECLESGQKYIFTPEDFQV"
     gene            5781..6440
                     /locus_tag="PLP58_17000"
     CDS             5781..6440
                     /locus_tag="PLP58_17000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009962173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="discoidin domain-containing protein"
                     /protein_id="HRJ10279.1"
                     /translation="MKNQALALAAIAALSSPVLADEVEVKIEFPKPMFIGTPVPAKLP
                     NLEQPDPSKIIKSFMAPAGTVNVASGKPVTSSDPAPIIGELELITDDDADGSDGCFVE
                     LAPGPQWVQIDLEASTTLSKIAMWHYHKQAQAYVAVVVQLSDDPEFKTGVTTVFNSDI
                     ENVHGFGEGKDPAYIETNHGRVIDAKGTKARYIRLYSNGNTSNEMNHYCEVQAFGVPG
                     K"
     gene            complement(6571..6858)
                     /locus_tag="PLP58_17005"
     CDS             complement(6571..6858)
                     /locus_tag="PLP58_17005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009962202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YMGG-like glycine zipper-containing protein"
                     /protein_id="HRJ10280.1"
                     /translation="MKQILLSILIAGGLVSCATGPNAQTGTVIGALGGAAVGGIIGNQ
                     SGRGLEGAAIGAGVGALGGNAIGNAQDQRNAQYYGQPQYQQPYYGPPPRRY"
     gene            7055..7168
                     /gene="rpmJ"
                     /locus_tag="PLP58_17010"
     CDS             7055..7168
                     /gene="rpmJ"
                     /locus_tag="PLP58_17010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003720928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HRJ10281.1"
                     /translation="MRVRTSVKPLCELCRVIRRKGVVRIVCKNPRHKQRQG"
     gene            7204..7599
                     /gene="rpsM"
                     /locus_tag="PLP58_17015"
     CDS             7204..7599
                     /gene="rpsM"
                     /locus_tag="PLP58_17015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009963700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HRJ10282.1"
                     /translation="MARLLGVDIPNEKKIEYSLCYIYGIGHPISQRVLTLANIDPNIR
                     AGELTDDQIALITQTVQGLKILIEGDLRREQQMHMKRLLSINCYRGNRHRRGLPVRGQ
                     RTHTNARTRKGPRKTVGAQRNPGAKKGKV"
     gene            7786..8187
                     /gene="rpsK"
                     /locus_tag="PLP58_17020"
     CDS             7786..8187
                     /gene="rpsK"
                     /locus_tag="PLP58_17020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003130556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HRJ10283.1"
                     /translation="MGGAETTEAKVVKAKGSKNVHSGIVHVRSTFNNTMVSVTDKVGN
                     LIGWSTSGKMGFRGSRKGTAYAAQVVAQDACRQAMGHGLREVEIRLRGPGSGRESAVR
                     AVQALGIEVTTIKDATPIPHNGCRPPKARRV"
     gene            8205..8816
                     /gene="rpsD"
                     /locus_tag="PLP58_17025"
     CDS             8205..8816
                     /gene="rpsD"
                     /locus_tag="PLP58_17025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004410410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HRJ10284.1"
                     /translation="MARYTGPREKIARRFGVPIFGPSKALETRNFPPGQHGGRNTRRK
                     QSEYGAALAEKQKLRFQYGVLEKQFRRFFAEAQRRKGVTGENLLQMLELRLDNVVYRM
                     GFANTRFASRQLVSHRHITVNGKIVNIASYQVKPGDVVAAKDTPRSQQLVGRFLEMTI
                     GSPIQDWMTVDRDKMSGVVNRAPIRDEINPIANEQLVVELYSR"
     gene            8884..9189
                     /locus_tag="PLP58_17030"
     CDS             8884..9189
                     /locus_tag="PLP58_17030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009960854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF971 domain-containing protein"
                     /protein_id="HRJ10285.1"
                     /translation="MTAPEKIEVIGKEVAIRWRDGAEDYITCERLRVASPSAENTGER
                     DLTGRVFGGTNQKSFSGVGVRSWRIVGGYAIQFEFTDGHNTGLYSFDYLRYICRHEV"
BASE COUNT         2019 a         2963 c         2891 g         1844 t
ORIGIN      
        1 catgagcgtg ctcatggccg gcgcgggcat ccgcggcggg cgggtggtgg gcggcacgga
       61 cgcgcgcgcg gagtatccga aggaccgccc catttacccg gaggacatca cgaagacggt
      121 ctattacgcc atgggcgtct ccgacctgga ggctcatgac cggctcggcc gcgcctacaa
      181 cctgctggag gatggcaggg cgctgacgga gatgtttggc taggccagca gggcctcgat
      241 ctcgcgcatc acctgcgcgc actcgacgat gagtgccggc tcgtagtcca tgccttggag
      301 gcgtttttgc aaaaccgcgt ggtccatgcg cccgccgcgg atcaaggcgg cgatggtgcg
      361 gatgtccttc atgccccggt ccacaccgcc gcgtcccatg cgccgagtcc agcgggagcg
      421 cgccgtggcg gccaccttgg tcaccgccat gtccacgggt tccaaggcga ggacatcctc
      481 aaagcccggc atcggcacca gccgcgcccg ccagccgtcc gggaaggatt ccgagagccg
      541 caggtccacg aagtcgccgt agtgaccggt ggcggcgtgg tattcgtggt ttttgccgag
      601 cgtgtcatgc aaatccaggc gcaccgcttc gtcgtccggg tccaggaaaa aatcggcgtc
      661 cagggtgacg gccacgccga tttgctcagg cgggacatct gggaaagatg ccagaagcga
      721 ggacgagccg aaaatcaaaa tccgccgccc cggcgccttc cgctggattt cgcggagcag
      781 ggagttcagt gagccgaggt ccatcggaag cgtttgagtt cctcgctgct gagcacaccg
      841 gggaaggggg agaattcttt gaagaacacc gcttccgctg aagaatcacg cagcagtgcc
      901 agcagggaac gcaagccgtc attcgaactc atggcggaga gcagcaccgc gcgccattgc
      961 tccagacggc tgaccgccga gtgcccgcgt gccagccagc ggtcaatgtt ttcgagcgga
     1021 atcctcagca gtgaggggtc ggcctcgatc ttgcctgcga tggcgcgaaa gacatccgcc
     1081 gacacctcat cccccggacg ctcggtccgc gagtcgagat aggcaaaagc ggttttggct
     1141 ggcatggcgg gactttgcca cgcggcgcgc ggcgcgccaa ggggcttttt ggccgcgctg
     1201 atatgtggcg ggcggcgcgg gcgtagtgtg gcgcatgaaa tttgtcaccg ccgcgctgct
     1261 gttgctcatc gtcatcacac ttcacgcgga ggactggccg cagtttcgcg gagtcaatgg
     1321 cagcggggtg tcggtgtcga ggggcttgcc gggggagttt tcggcggaga aaaacgtgct
     1381 gtggaaggcg cggctgggtg acggcgtcgg gtcggccatc atcaaaggtg gggtggtttt
     1441 tgccaccggc atggcggggg agggcagggt ggctttgcac gcgctggatg ccgcttcggg
     1501 ggcggtgaaa tggaggtcgg agttcgagac gggcacgctg ccgcgcatca caccgccgaa
     1561 cagccacgcg gcttcgacac ccgcggcgga cggggagcgt gtgtatgtgc atttcagcac
     1621 catcggcctg ctggctttcg acgcggcgac gggaaaggaa gtctggcggc attccatgcc
     1681 gcgcccggct tacctgatgg actggggcgc ggcggcatcg cccgtcgtgc atgaggggaa
     1741 ggtgattttt tgccaggacg acgacctggc gcccttccta gtggcggtgg atgcggcgac
     1801 gggccgcgag gtgtggaaga cgccgcgccc ggacatgctg gccggttacg ctttgcccgt
     1861 gctctgcgag gcgggcgggc gtgtggatct cgtggtggcg ggcagcggca agatgaaggg
     1921 atacgatccc gccacggggc gcgagctgtg gacctgcaac acgctgctgc gcaccatcat
     1981 gacctcgccc gtggtgcatg agggtgtcgt ttacctcgcc gtgcagagtt acggcgactc
     2041 cacgcgcacg ctgaagcacg cgctgctcga atggctggac acgaaccagg acggcatcct
     2101 ggcgcgcgag gagacgccga aggaattcca cgagcgcttt gacgcctccg acaaaaacaa
     2161 aaacggcctc attgatcccg aggagataga caccgccttt caaagcccgg acaacatggc
     2221 cgccgggggc aacatcatcc aagccatcaa aggcggcggc agcggtgatg tgacgaagac
     2281 gcacgtgctg tggaacctgg atcacaagac gccgtcgaac atcgcctcgc ccctgcttta
     2341 ccatggccgg ctccacatcg tgaagggcgg cggcatggca agctgctacg acgccaaaga
     2401 cggcgcgcag ctctgggacc gtgtgcgcct gggcaatttc ggggattatt tcgcctcgcc
     2461 cgtggccgct gacgggaaga ttttcatcgc gggaaaaaac ggtttcgtcg tcgtgctgga
     2521 ggacggggcg gagctgaaag tgctgggcaa aaacgacatg ggcgaggaga tcatcgccac
     2581 gccgagcatc gcggacgggc ggctctacat ccgcacgcgg gagaatcttt tctgcatcgc
     2641 cagcggcggc tcgaaggctg tgcttgcgga tagcgcagcg gtcgccgcgc ctgtggagat
     2701 gactttgagt ccggtggtca gcggcagggt gtggaatggc tacacgggca acgcgatggg
     2761 ccaggaatcg tggagcgagg cggagctgga gcagcgtctc gaacagctca aaaagctcgg
     2821 ctacaccacg gtggccgtgc cgggaaaaat cgcgcccttt gcgccgatcc gcgtggatgg
     2881 ggacacgggt ggccgcaagg ccttccgggg tgcgaggacc ttcgaaaacc cggacgtggc
     2941 cgccatcacc acacgcttcc gcgaaaaggc cgccaaactg ggctttgagg tcgtcaccgc
     3001 cgatcctgtg gcggtgtctg tgctgccggg gaatgagttt cctgacgaga aaaccctcgc
     3061 cgcgcttgtc actccgctct gtggcgaggg ggtggctgcg cgcatgtggc tgggctttca
     3121 ggccgtggac aaagccgcgc ggctcattgc cgaaaacgat ccagcccttg gcattcccgc
     3181 gccggacatg atcacccggc atttgaatgc cggagaggcc ccgccggcct ggctcgtcga
     3241 agtcaaggga ctctatgcca ccgcgatgaa cgagatgtac cgcgccaaca cccgtgctcg
     3301 ggaaggctcg cgcagcttca cgctttacca ggccaagcgc atggagtccg cctttcactt
     3361 tctcaacgcc attgaggcta tgcacaaagc ggcggctgac gccaccatcc gcgccgaatc
     3421 cctcaccgcc gcgcaggaga gcctgtacaa cgcgctcaac gcctggtccg acgcggcccg
     3481 ggattccagt gaccgcgggg ccatcgccct gctcaacgag cacggctacc gggtcttgaa
     3541 aaaacaggcg gcgcaaggtg aatgagcgcg gtcgtggagc cgatgaacac tttgcaatcc
     3601 tgagaacggg gcgattttag cccttctaat ttccactgga tttctgaatg cagccgccca
     3661 agactcacgt tcccaacctt ccaattcacc gaaaatatca tgtctgacac gcccgccacc
     3721 tcctgctgct cgcgcctcga cctcgcgttt gccaacctga tcgcccgtct ctgggtggcc
     3781 ttgcgtcttg tcatggccgg tgtggacaaa ttccgcgccg gtgatggtgc cgaggccact
     3841 ttcagcacgg ccaactacga gaccaaaacc aagctcatca gcgacctgat gacccagaac
     3901 agcttcctcc cggcttttct ccctgcctcc gccattgaca tgtacgcgca cagcatcggc
     3961 tacctgctca tcgccgtcgg tctttgggtg gccgtgggcc tgctcagcga gttcgccctg
     4021 gtcgccgccg gcctgacatt cctttctctc ggcttcgggc tcgccgcctt gccggatgat
     4081 tccgagctga ccatcaacat cggcatcggc atcatgatca ccttcatggc cctgatgacc
     4141 aacaaatgcg cctggttttc cctggacgga ttgttcggac gccaccgcgc caaaaaggtc
     4201 gtcgtcacgg aagcctgagc cgccgcgcgc ccgtatcctg atcgaatcat gagcacttcc
     4261 tcagccactc cttccacggg gctgtccaga ttcatggacc gccgttcttt tctgcgcacc
     4321 agcgccgtca gcggcgctgg cctctacatt gccaccagca agagcgccat cgcccagggc
     4381 gagcaggccg ggcgcaccat caaatgcgcc ttgatcggct gcggtgccca gggggaccgt
     4441 ctgcgtgtcg cttcctcgca gatcaagacc ggcatccagt gggtcgctgt cgcggacatc
     4501 tggaaataca accgcaatcc tgtggcacgc cggatggagt ttgaaaacaa gcaccaggtg
     4561 gaagggccgg tggcgcagta tgaggaagtc gaggaaatgc tcgacaagca tcccgagatc
     4621 gaagccgtct tcattgccac ccccgaccat cttcacgcgc cattctcccg cctttgcctg
     4681 gaaaaaggca agtccgtgta ctgcgagaag atgatgtcga acaccatcga aggcgcgcgg
     4741 gacatggtga aagccgggcg tgaaaacaac ggcatcttcc agattggcca ccagcgccac
     4801 tcgaaccccc gctacatcca cctgcgcgag aagatcctca aaaaaggtct tcttggccgc
     4861 gtcactcatt gctacggcca gtggaaccgc ggcgtcgccg ccagccagcc gctcggcatt
     4921 ccgaaaaacc agaccatacc cgccgagatg ctggagaagt acggcttcgg cagcatggag
     4981 gagttccgca actggcgctg gttcgccaaa tacggcggag gtcccatttc cgaccttgga
     5041 gcgcaccaga ttgacatgta caactggatg tacgagacca ctcctgtgtc gctttttgcc
     5101 gccggcggtg tggactacta tgacggcaca ccgggtcctg atggcgagcc gaaggcgaag
     5161 tttgagctgc cggacaacgt gatgtgcctg tatgagtacc aaacgaagga aggcgtcatg
     5221 cgcgcctact accaggtgct caccaccacg ggcagccagg gctattatga aaaacacatg
     5281 ggtgtcgcgg gttcggccat catttccgaa agccccacct acaaccaggt ctatgccgaa
     5341 cccggcagcg actggagcca gtacgccatg ggggacgacc ccatcctggt gaaagcaccg
     5401 gatgccgtga agaacaagtt ttgggagcat ccgcgctcct gggcaaaacc ggtgccgaaa
     5461 tcctacaacg tgaccggtgt cgccaaagcc gtcgccgatg cgcgcgaatc caaggcgctg
     5521 gatccgtggg agatccccgt cattcttgaa gtctatccgc acacgccgca tgtcgccaat
     5581 ttcattgagg ccgtgcagat gaagaagccc gcacacctga cctgcaacgt catggatgcc
     5641 ttcaaatcct gtgtcaccgt tctcaaagcc tacgaatgcc ttgagtctgg acagaaatac
     5701 attttcacac ccgaagattt ccaagtctga gttcgtccta ccctgcggct caaagccatc
     5761 aaacccatcc cccacacctc atgaaaaacc aagccctcgc cctcgctgcc atcgcagccc
     5821 tcagcagccc ggtccttgcc gacgaagtcg aagtgaagat cgagtttccc aagcccatgt
     5881 tcatcggcac ccccgtgccg gccaagctgc ccaatttgga gcagcccgat cccagcaaga
     5941 tcatcaagtc cttcatggcc cccgctggca cggtcaacgt cgccagcggc aagcccgtca
     6001 cttccagtga tcccgccccc atcatcggtg agttggaatt gatcactgac gatgatgcgg
     6061 atggctccga tggctgcttc gttgaattgg cacccggccc gcagtgggtg cagattgacc
     6121 tggaggcctc caccaccctc tcaaaaatcg ccatgtggca ttatcacaag caggcccagg
     6181 cctatgtggc cgttgtcgtc cagctttccg atgatcccga gttcaagacc ggtgtcacca
     6241 ccgtcttcaa ctctgacatt gagaacgtcc acggcttcgg agaaggcaaa gatcccgcct
     6301 acatcgaaac caaccacggt cgtgtcattg acgccaaagg caccaaagcc cgttacattc
     6361 ggctctacag caacggcaac acttccaacg aaatgaacca ttattgcgag gtgcaggcgt
     6421 tcggtgttcc cggcaaataa gcggccgccc cgcccgattc aagaacccgt tgccgaccag
     6481 ccggcaacgg gtttttttgc gccattttcg gacgaaaaaa agcgcggacg gtaagcccgt
     6541 ccgcgctgaa tgtgtcgcca gtcacggtga tcagtaacgg cgcggaggcg gcccgtaata
     6601 cggctgctga tattgcggct gcccgtagta ttgcgcgttg cgctggtcct gggcgttgcc
     6661 gatggcattg ccgccgaggg cgccgacacc agcaccgatg gcggcaccct cgagtccgcg
     6721 gccgctctgg ttgccgatga tgccaccgac agcggcgccg ccgagggcgc cgatgacagt
     6781 gcctgtctgg gcgttcggac ccgtggcgca ggagacgagg ccgcctgcga tgagaatgga
     6841 gagaaggatt tgtttcatat gctttggtgt gtcgtggttg accctgcaat tattcacaaa
     6901 ctttgcgaaa aagacagggt atttcatgat tgttacgaaa taagaagacg aagtcgccgg
     6961 atttcaaaaa gtccaaaatc agccttgcgt gccaagactc ctgtgttagg acttccggct
     7021 ccccgtctcg cggggaacac acacttttgc ctccatgcgt gtacgtacct ctgtcaaacc
     7081 tctctgcgaa ctctgccgcg tcattcgccg caaaggcgtt gtccgtattg tctgcaaaaa
     7141 cccccgccat aagcagcgcc agggctgatt cttcatcttt gaacttcaaa aaatcccgat
     7201 tttatggccc gccttctcgg agttgacatc cccaacgaaa aaaagatcga atattcgctg
     7261 tgctacatct acggcatcgg tcatcccatc agtcagcgcg tgctcaccct ggcaaacatt
     7321 gaccccaaca tccgcgctgg tgaattgacg gatgatcaaa tcgccctcat cacgcagact
     7381 gttcagggct tgaagatttt gatcgagggc gacctccgcc gcgagcagca gatgcacatg
     7441 aaacgcctgc tgagcatcaa ttgctatcgc ggcaaccgcc atcgtcgggg gctgcccgtg
     7501 cgcggacagc gcacccatac gaacgcacgt acccgcaagg gaccgcgcaa aaccgtcggt
     7561 gcccagcgca accccggcgc caagaagggc aaggtctgat ccgtctcgaa attttgaact
     7621 gaaactgaat acgatccttc catggcagac gaaaacctga atcccgaagc cgcagctccc
     7681 gctcctgaag ccgcgccgac tccggcccca gccgccgctg cgcctgcggc tgacaagcaa
     7741 ccctccagcg gcgaccgcgc tgtttcccaa agtgtctggc tcgaaatcgg cggtgcagaa
     7801 accacggaag ccaaggtcgt caaagccaag ggatccaaga atgtgcattc cggcattgta
     7861 catgtgcgct ccactttcaa caacaccatg gtgtccgtga ctgacaaggt cggcaacctg
     7921 atcggatggt caacatccgg gaaaatgggt ttccgcggat cccgcaaagg caccgcttac
     7981 gccgcccagg ttgtggcgca ggatgcatgt cgccaagcca tggggcatgg tttgcgcgag
     8041 gttgaaatcc ggcttcgcgg gcccggttcc gggcgtgaat ccgccgtgcg cgcggttcag
     8101 gcgttgggca ttgaagtgac caccatcaaa gacgccactc ccatcccgca caacggttgc
     8161 cgccccccca aagcccgtcg cgtctaattt aatttttctc ctttatggca cgttacaccg
     8221 gtccccgcga aaaaatcgcc cgccgcttcg gagtcccaat tttcggtcct tccaaagccc
     8281 tcgaaacccg caattttccg cctggccagc atggcgggcg caatacccgc agaaagcagt
     8341 ccgaatacgg agccgcgctc gctgaaaagc agaagctgcg ctttcaatac ggcgttcttg
     8401 aaaaacagtt ccggcgcttc tttgccgaag cgcagcgccg caagggtgtg acgggtgaga
     8461 atctgctgca aatgctggaa ttgcgcctcg acaatgttgt ttatcgcatg ggattcgcga
     8521 acacgcgctt tgcttctcgc cagctagtca gccatcgcca catcaccgtc aacggcaaga
     8581 tcgtcaatat tgcgagctat caggtgaagc cgggcgatgt ggtcgccgcc aaggacaccc
     8641 cccgcagcca gcagcttgtg ggacgttttc tggaaatgac catcggctca cccatccagg
     8701 actggatgac tgtggatcgc gacaagatgt ctggcgttgt caatcgtgcg cccatacgcg
     8761 acgagatcaa ccccatcgcc aacgaacagt tggtcgtgga gctttactcc cgttgatatt
     8821 tccagaaatc acgcgcgaaa gggacgcttt tcggcgtccc tttttcgtgt tgccatcgcg
     8881 aatatgacag cacctgaaaa aatcgaagtc atcggcaagg aagtcgctat tcgttggcgg
     8941 gatggcgcag aggactatat cacctgcgaa cggttgaggg tggcttctcc cagcgcagaa
     9001 aacactggcg agcgtgattt aaccggacgt gtgtttggcg gcacaaatca aaagagtttt
     9061 tccggagttg gcgtgcggag ttggcggata gtcggtggat acgccattca atttgaattc
     9121 accgacggac ataacacggg cttgtattcc tttgattatc tcagatacat ttgccgtcat
     9181 gaagtgtagc gtcttttgaa tttgaatgtt gcaaagtgga aaacagtttt ggttgtagcg
     9241 ctacggtttc gcggcatcaa actagccaca agcgctagcg aaacgtgtcc ggcagcttta
     9301 ttcgctttca acccggcaag tcatgcaggg gactagaagc ggtttgtgcg tttcgcagag
     9361 aactcatcgg ctggcaaaag ggaaaggcat cacacgcttg caggctctcg cgcccttggt
     9421 ggcatcacac acacgcgaaa accttcatag gactctggag gatcttcgag ccgcccgtta
     9481 tttgcggcgg gacgaaattc ccatcaaatt tgctttgaca gaaagagaag aaaccatatg
     9541 gtcacgcact ttttctccaa aagcaggggt gtattgtctc ttgacgccgc gcaacaggca
     9601 atttacaccc ctccttttcg tcgaaagagg aggaggttct cacgagccgc cgcagggtta
     9661 aagctgatgt agctcagtgg tagagcacgt ccttggtaag gacgaggtcg agggttc
//
LOCUS       DAOMYJ010000195         9702 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Prosthecobacter sp. isolate AMR_MDS_5629
            NODE_2334_length_9702_cov_22.452991, whole genome shotgun sequence.
ACCESSION   DAOMYJ010000195 DAOMYJ010000000
VERSION     DAOMYJ010000195.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867850
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prosthecobacter sp. (wastewater metagenome)
  ORGANISM  Prosthecobacter sp.
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Verrucomicrobiaceae; Prosthecobacter.
REFERENCE   1  (bases 1 to 9702)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9702)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.30
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:34:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,742
            CDSs (total)                      :: 4,684
            Genes (coding)                    :: 4,676
            CDSs (with protein)               :: 4,676
            Genes (RNA)                       :: 58
            tRNAs                             :: 55
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9702
                     /organism="Prosthecobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2334_length_9702_cov_22.452991"
                     /isolate="AMR_MDS_5629"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1965333"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(198..1700)
                     /locus_tag="PLP58_17035"
     CDS             complement(198..1700)
                     /locus_tag="PLP58_17035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009962885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ10286.1"
                     /translation="MDRRDFFKTTAAAGMAGALPFAGAVSPRQPGLIRAENARPGSSF
                     QLTRMRPDDAKSHRTSLIEGYCSRQSLIAGEKLDIFVSTKPAARFIIEVFRAGYYGGE
                     GARLMTTLGPFDGMEQPVPEIGENRLRECRWQASSSLTIPGDWPSGVYLGRLTTLPES
                     ADKPYWQSYVIFIVKDDRPADILFQCSDNTWQAYNRWPVNESLYTDPRAAHAPGVSCS
                     FDRPYGKYVQIFDNPQSMGSGEFLLWEYPLCYWLEKEGYDVTYCSNVDNLDPANLSRV
                     KTMISTGHDEYWDVRQFHNVKQAIADGLSVLWLSANDVYMVTPFTPNAKGDANRRLTR
                     ETCFGEFREEEKEAYSKVLGPFENTGPDEREIIGARTVVPFNGGGDWTCAMPDHWLFE
                     GTGMKKGESIPGLVGWEFHGDPDTARAGLEVIAEGNVWAGGTRLGKYAATIFEGPKKN
                     IVFNATTIFWSQGLSDPPGHMIPWSHYSRPHGPDERVQRITKNALKRAIG"
     gene            1867..2769
                     /locus_tag="PLP58_17040"
     CDS             1867..2769
                     /locus_tag="PLP58_17040"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HRJ10287.1"
                     /translation="MRVPALFLLLAAAATAAPRAGHVIVVSIDGANCELVQKAEMPNL
                     DRLVVEGAHTWRARTIIPSKTLPSHTSMLTGLDIARHQIDWNEYFPLRGLVKTPTVFS
                     LAKQAGIENITGLFCGKIKFRHLWLKDSLNVFDCGGEYDYGPIPAEEEKKLVPAAEVA
                     RRAAAWIVERKPRLCFIHLPDVDINGHKHGWGSDEQMEAFKACDQALGRVIEAVGKAG
                     IAEDTTVIITADHGGKDKSHSDPIPNNTRIPWIAWGKGVRKGSEITGREPMTYDTAAT
                     VLWLLDIPAPDPLDGRPVTEAFDE"
     gene            2919..3962
                     /locus_tag="PLP58_17045"
     CDS             2919..3962
                     /locus_tag="PLP58_17045"
                     /inference="COORDINATES: protein motif:HMM:NF013862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HRJ10288.1"
                     /translation="MITNLNPIRPGWMRNAEDPVCGGGAPSLEKQAPRIGLALSGGGA
                     RGLAHVGVIEVLEETGIPIAAIAGTSMGAYVGALYAAGCGPSDLESLAREIRDRRTLL
                     RLLDPVFPPTTGLIRGMKIRRHLERTLGSRSFEELEKPLLVVAADLDSMAAHVFDSGP
                     VGPAVHASAAIPGLCAPVHIDGRRFTDGGAVEPLPVGLLRERFALDHVIAVNVMPTPE
                     DIRSCPKGMFSPPDTKSPRPGYWLRRLLRPFNLLAFGNVLDTFRRALMCAQLGLVEKE
                     CRSADVVVHPFFCESTWFDFENFDRYIRAGRAAAVTALPRILALLGRPTPDLEKHHEN
                     PASIHPDLGLRAA"
     gene            3913..5406
                     /locus_tag="PLP58_17050"
     CDS             3913..5406
                     /locus_tag="PLP58_17050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009962689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HRJ10289.1"
                     /translation="MKILHPSIPTWDSAPPEVWREWQLRRLRDYLAKRVLPYSAHYRR
                     LFAEHDLHPADLKTLADWSDAPFTSKGDLTVPREQQREFVLLPDEAALKREWDVILHA
                     LMHGRASTQAALDHEFRPVLLTSTTGRSSEPVPFLYTKHDLANLDITGRRLMECGRSQ
                     KDFRHLNAFPFAPHLAFWQAHHAGLSFGTFMVSTGGGKAMGTEGNLRLMDKIQPDVVI
                     GMPTFLYHLLRQGVEKGAHWPNIKRVVLGGEKVADGLRARLVDLAAELGSEGAHVIST
                     YGFTEAKMAFPECPAGIEASGFHLSPDLALIEIVDPVTGQPAEDGEPGEIVFTPLDAR
                     GTVVLRYRTGDIASGGLTWEPCPHCGRRCPRLRGPIGRVSEVRELHLDKVKGTLVNFN
                     ILEHLLDDQKGVAAWQIELRKRDDDPHEVDELVLHVTPENGVNTDDLEHRVVNRFAEV
                     TEIRPNAVRFHDMPEMRERLGVGTLLKEAKLLDHRPKTPEREAALSS"
     gene            5432..6709
                     /locus_tag="PLP58_17055"
     CDS             5432..6709
                     /locus_tag="PLP58_17055"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009962690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003985 - acetyl-CoA C-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase"
                     /protein_id="HRJ10290.1"
                     /translation="MKPLHIISAIRTPFTRAGTVLAGQDAVDLGKAAVSALLTRGGFD
                     PQVVDEVIFGCVGQPAHAQNIARVIALRAGIPEACPAMTVHRNCASGLEALTTAHAKM
                     CAGQGEVFLVGGTESMSNMPLYFSRLAAEKFSTLGKARGLGQKLGALTSFRPADFAPI
                     IGLKLGLTDPVVEMNMGETAELLAREFHISRDMQDAFALRSHQRALAAAEKLRAETAP
                     LYLADHGFHPVLSDNGVRADSSLEKLARLKPMFDRHHGTVTAGNSSQITDGAVALLVA
                     SEEAASRHGWQSLGKLTHYAYTGCDPRRMGLGPVRALDAVLHHSGMRLEAADVIEINE
                     AFAAQVLAVLKCLKDPACARRAGLDAPLGEIADEQLNPRGGGIALGHPVGATGARLVL
                     TALDQLRESGGKRALVSLCIGGGQGGAALLERD"
     gene            6741..8855
                     /locus_tag="PLP58_17060"
     CDS             6741..8855
                     /locus_tag="PLP58_17060"
                     /inference="COORDINATES: protein motif:HMM:NF012596.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase NAD-binding
                     domain-containing protein"
                     /protein_id="HRJ10291.1"
                     /translation="MKTELLQSLEKNHEDAFIPRPPSPMIRAKSLRHFRLDSDEDGTA
                     WITFDMEGTAANVWNETTLHEFDLCLEAVSRDSTLKTLVIQSAKEHIFIAGADLKAVR
                     HAPVRQLETLIGLGQKVFNRLAALPLHKIALIHGACLGGGLELALACDTRIVSDSDRT
                     RLGLPETQIGLVPAWGGSTRLPRLLGLPAALQLIVTGKLLKPSAAKRAGLADFIVPRE
                     RFEDFVHHRIALGAPKRRLSLTGRISLLPGARHFIASQVKKAVLTKTRGLYQAPLRAV
                     EVTMRASVAPLPKAMEIERHAIQDLALTPATGHLIDLFFRKEEADKKPWPRGVALPVH
                     HAAVVGAGVMGAGIAHWLASRGVRVLMQDISTDALARGMDRMHGLLKEGVKRRAISRV
                     QMRDTLDRLTAEHTRVPLTHEQIIIEAATEDMALKKKIFADLAARCGPDTVLATNTSA
                     LSVTELAETLPHPERVIGLHFFNPVHRMPLVEIITTPHTSDDVIATATAFVQKLGKTP
                     VVVRDSPGFVVNRVLMPYLMEAVRLYESGIPAETIDEAMLEFGMPMGPLRLLDEIGLD
                     VAAHVGRTLVAAFPDHLAASDLLDRMVSTGRLGKKSGEGFYKHGARVKRKTVKHDASE
                     LEHVQHRLALLLANEAARCAREKLTQPPESIDLAMILGTGYPPFLGGPLAWLRELGAE
                     NAKLELQMLAATTPQPNAFEPA"
     gene            complement(9160..9627)
                     /locus_tag="PLP58_17065"
     CDS             complement(9160..9627)
                     /locus_tag="PLP58_17065"
                     /inference="COORDINATES: protein motif:HMM:NF025080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HRJ10292.1"
                     /translation="MPGHTYRILVTNRDEDHVTLCRDYNQSACIEQRIEELKNDLAAD
                     GFCMRRSYATEAAFLCVIFAFNLVSLYQQATGTRSEKHYQGPATLRSTVFMGGAILGK
                     RGRQPVLHIAQSWAGAEKHQPLMGNLLAWRKRTSPKLEPPVATPPLNNLKNVA"
BASE COUNT         1780 a         3239 c         2954 g         1729 t
ORIGIN      
        1 aggcgtcctc gtatttggcg gcctgataac agtgctccac actgcgccac ttcacaccgc
       61 taaggacgaa gccatgaggt gacaagttcg aaagccagtc ggtggccggg ttcttggaat
      121 ggaaaagcag tgccatgccg gctgacatga atgccgcccg gcgcaagatg caactgacat
      181 gctcgccggc acccgagtca tccgatggcc cgcttcagcg cattcttggt gatgcgctgc
      241 acgcgctcat cggggccatg cggccgtgaa tagtggctcc acgggatcat gtggccgggt
      301 ggatcgctga ggccctggga ccagaagatg gtggtggcgt tgaagacgat gttcttcttc
      361 ggaccttcga agatcgtggc ggcgtatttg ccaaggcgcg tgccgccggc ccagacattg
      421 ccctcggcga tgacttcgag gcctgcgcgt gcggtatctg gatcgccgtg gaattcccag
      481 ccgacgaggc cggggatgct ctcgcccttt ttcatgccgg tgccctcgaa gagccagtgg
      541 tccggcatgg cgcaggtcca gtcgccgccg ccgttgaagg ggaccacggt gcgcgcgccg
      601 atgatctcgc gctcgtcggg gccggtgttt tcaaaggggc cgagaacctt cgagtaggcc
      661 tctttttcct cctcgcggaa ctcgccgaag caggtctcgc gcgtgaggcg gcgattggca
      721 tcgcctttcg cgttcggcgt gaagggcgtg accatgtaaa cgtcgttggc cgatagccag
      781 agcacgctga ggccgtcggc gatggcctgc ttgacgttgt ggaactggcg cacgtcccaa
      841 tattcgtcgt ggccggtgct gatcatcgtt ttgacgcggg agaggttggc gggatcgaga
      901 ttgtccacgt tcgagcagta ggtcacgtcg tagccttctt tttccagcca gtagcacagc
      961 gggtattccc acagcaggaa ctcgccgctg cccatgctct gcgggttgtc gaagatctgc
     1021 acgtatttgc cataaggccg gtcaaagctg caactgacgc caggtgcatg cgcggcgcgc
     1081 ggatcggtgt agagcgactc gttcacgggc cagcggttgt aggcctgcca tgtgttgtcg
     1141 ctgcactgga agaggatgtc ggcagggcgg tcgtctttga cgatgaagat gacgtagctc
     1201 tgccagtatg gcttgtcggc ggattcggga agggtggtga ggcggccgag atagacgccg
     1261 ctgggccagt cgccggggat ggtgagggat gaggatgcct gccagcggca ctcgcgcaga
     1321 cggttttcgc cgatctccgg cacgggctgc tccatgccgt cgaaggggcc gagcgtggtc
     1381 atgagacggg cgccctctcc gccgtagtaa cccgcgcgga aaacctcgat gatgaagcgt
     1441 gcggcgggtt tggtgctgac gaagatgtcg agtttttcac ccgcgatcag cgattggcgc
     1501 gagcaatagc cctcgatcaa ggaagtgcgg tgggactttg cgtcgtccgg cctcatgcgg
     1561 gtgagctgga aactggagcc gggtctggcg ttttccgcgc gaatgagccc aggctgacgc
     1621 ggtgacacag cgcccgcaaa cggcagcgcg cctgccatgc cggctgctgc ggtggtttta
     1681 aaaaagtcac gacggtccat gagcagcaat acgggagcag ggcggcgcgt atagcaggcc
     1741 gtgatccgcg cgtcttgtcg cttcggccaa cagcgggatg cccgcagcgc agttaaaacg
     1801 agcagttgac ggaaaagacg ctggcattgc cggcggcggg cggcatgatt ccagcctcgt
     1861 ccattcatgc gcgttcccgc cctttttctc cttctcgccg ccgctgccac cgccgcgccg
     1921 cgcgccggcc atgtcatcgt cgtcagcatt gacggggcga actgcgagct ggtgcaaaag
     1981 gcggagatgc cgaacctgga ccgcctggtg gtggagggcg cgcacacctg gcgggcgcgc
     2041 accatcattc cgtccaagac gctgccctcg cacacctcga tgctcacggg gctggacatc
     2101 gcgcggcatc aaattgactg gaacgaatat ttccccctgc gcggccttgt gaagacgccc
     2161 acggttttct ccctcgccaa acaggccggc attgaaaaca tcacggggct gttttgcggc
     2221 aaaatcaaat tccgccactt gtggctcaag gactcgctca atgtctttga ttgcggaggc
     2281 gagtatgatt acggccccat ccccgccgag gaggagaaga agctggtgcc agcagcagag
     2341 gtggcccggc gcgcggcggc atggattgtg gagaggaagc cgcggctttg cttcattcac
     2401 ctgccggatg tggacattaa cggacacaaa catggctggg gttccgacga gcagatggaa
     2461 gcgttcaagg cctgcgacca ggcgctgggt cgcgtgattg aggcggttgg gaaagcgggc
     2521 atcgcggagg acacgacggt catcatcacc gcggatcacg gtggcaagga caagagccac
     2581 tcagacccca tcccgaacaa cacacggatt ccctggatcg cctggggaaa aggcgtgcgc
     2641 aaaggaagcg aaatcaccgg gcgcgaaccc atgacctatg acacggcggc gactgtgctg
     2701 tggctgctgg acatccccgc gcccgatccc ttggatggcc gccctgtgac cgaggctttc
     2761 gatgagtgat gccgcgcgtc catgcgcatg agcacaccac gtcgcatccg atgattcaca
     2821 cccgctcagg catgtccggg cggagttgaa ccggcgtcag ctcttgaaca aaagcgcccg
     2881 ttgttttgtc tcatatccca agaaaggagg tatgaaacat gatcaccaat ctcaatccaa
     2941 tccgtcccgg atggatgcgc aatgcggagg atccggtatg cggtggtggt gcgccctctt
     3001 tggaaaaaca ggctccgcgc atcgggcttg cgctctcagg cggtggcgcg cgcggcttgg
     3061 ctcatgtggg cgtcattgag gtgctcgaag agacaggcat ccccatcgcc gccatagcgg
     3121 gcaccagcat gggcgcttat gtcggcgcac tttacgccgc aggctgcggc ccctcggatc
     3181 tggaatccct ggcgcgcgag attcgtgacc ggcgcacact gctgcgtctg ctagaccctg
     3241 ttttcccccc aacgaccggc ctgattcgcg gcatgaaaat ccgccgccat ctggagcgca
     3301 cgcttggctc gcgcagcttc gaggagctcg aaaaacccct cctcgttgtg gccgccgacc
     3361 tcgacagcat ggccgcccat gtctttgaca gcggtcctgt cgggccggcg gtccatgcca
     3421 gcgcggccat tcccggtctc tgcgctcccg ttcacattga cggacgccgc ttcaccgatg
     3481 gcggcgcggt ggagccgctg cccgtgggcc tgctgcgcga gcgcttcgcc ctggaccatg
     3541 tcatcgccgt caacgtcatg cccacgccgg aggacatacg ctcctgcccg aagggcatgt
     3601 tttccccgcc cgataccaaa agcccgcgcc cgggctactg gctgcgccgc ctgctgcgcc
     3661 ccttcaacct cctggccttt ggcaacgtgc tggacacttt ccgccgcgct ctcatgtgcg
     3721 cgcagcttgg cctcgtggaa aaggaatgcc gctccgccga tgtggtggtg catcccttct
     3781 tctgcgagag cacatggttc gactttgaaa atttcgaccg ctacatccgc gccggacgcg
     3841 cggccgcggt caccgccctg ccgcgcatcc tcgcgctgct cggacgcccc acacccgacc
     3901 tggagaagca ccatgaaaat cctgcatcca tccatcccga cctgggactc cgcgccgcct
     3961 gaagtttggc gcgagtggca gctccgccgc ctgcgcgact acctcgccaa gcgcgtcctg
     4021 ccctacagcg ctcactaccg acgcctcttc gccgagcatg atctgcatcc cgcggattta
     4081 aaaacgctcg ccgactggag tgacgcgcct tttacctcca agggcgacct caccgtgccg
     4141 cgcgagcagc agcgcgaatt cgtgctcctg cccgatgagg ccgcgctcaa gcgcgagtgg
     4201 gatgtcatcc tccacgcgct catgcatggc cgcgcttcga ctcaggcggc gctggaccat
     4261 gagttccgcc ccgtgctgct caccagcacc actgggcgct cctccgaacc ggtgcccttt
     4321 ctctatacga agcatgacct ggcgaacctc gacatcacag gccgcaggct catggaatgc
     4381 gggcgctcgc agaaggattt ccgccatttg aacgccttcc ccttcgctcc gcatctggcc
     4441 ttctggcagg cgcatcacgc cgggttgagc ttcggcacct tcatggtcag caccggcggc
     4501 ggcaaggcca tgggcacgga gggaaacctg cgcctcatgg acaaaatcca gcccgatgtc
     4561 gtcatcggca tgcccacctt cctttatcat ctgcttcgtc agggcgtcga aaaaggcgcg
     4621 cactggccta acatcaagcg cgtcgttctc ggcggtgaga aagtggccga tggcctgcgc
     4681 gcccgcctcg tggatctggc cgccgagcta ggctccgaag gcgctcatgt catcagcacc
     4741 tacggtttca cagaggcaaa gatggctttt ccagaatgcc ctgccggcat tgaggccagc
     4801 ggcttccacc tcagcccaga cctagccctg atcgagatcg tggacccagt caccggccag
     4861 cctgcggagg atggcgagcc gggagagatc gtcttcaccc cgctcgacgc gcgcggcacc
     4921 gtggtgctgc gctaccgcac cggagacatc gccagcggcg gcctcacctg ggagccatgt
     4981 ccgcattgcg gacgccgctg cccgcgcctg cgcggcccca tcggccgcgt cagcgaagtg
     5041 cgcgagctgc acctcgacaa ggtcaaaggc acgctcgtga acttcaacat cctcgagcac
     5101 ctgctcgacg atcaaaaagg cgtggccgcc tggcagatcg agctgcgcaa acgcgacgac
     5161 gatccgcatg aggtggacga gctcgtcctg cacgtcacgc ccgaaaacgg cgtcaacaca
     5221 gacgacctcg aacaccgcgt ggtgaatcgc ttcgctgaag tcaccgagat ccgcccgaac
     5281 gccgtgcgtt tccacgacat gcccgaaatg cgtgagcgcc tcggcgtcgg cacgcttttg
     5341 aaagaggcca agctcctaga ccaccgcccg aaaacacccg agcgcgaagc cgcgctcagc
     5401 tcttgaagcc aatccttgcc ccataaaagc catgaaacct ctccacatca tctccgccat
     5461 ccgcactccc ttcacccgcg cgggcaccgt cctggccggg caggatgcgg tggacctcgg
     5521 caaggccgca gtctccgcgc tgctgacacg tggcggcttt gatccgcagg ttgttgacga
     5581 ggtcatcttc ggctgtgtcg gccagccggc gcacgcgcag aacatcgcgc gtgtcatcgc
     5641 cctgcgcgcg ggcatcccgg aggcgtgtcc ggccatgacg gtgcatcgca attgcgcctc
     5701 cggcctggaa gcgctgacca ccgcccatgc caagatgtgc gccgggcagg gagaggtctt
     5761 cctcgtcggc ggcacggaga gcatgagcaa catgccgctc tatttttcac gcctggctgc
     5821 ggagaaattc agcacgctgg gcaaggcgcg cggccttggg caaaagctcg gcgctttgac
     5881 ctccttccgc cctgccgact tcgcgcccat catcggcctc aagctcggcc tcaccgaccc
     5941 cgtggtggag atgaacatgg gcgagaccgc cgagctgctg gcccgcgagt tccacatcag
     6001 ccgcgacatg caggacgcct tcgccctgcg cagtcaccag cgcgccctgg cggcggcgga
     6061 gaagctgcgc gcggaaacgg cgccgcttta tctggcggat cacggctttc atcccgtgct
     6121 ctccgacaac ggcgtgcgcg cggactccag cctggaaaaa ctggcgcgcc tgaagcccat
     6181 gtttgaccgc catcacggca cggtcacagc gggcaattcc agccagatca ccgatggtgc
     6241 ggtggccctg ctggtggcga gcgaggaggc cgcctcacgt catggatggc aatctctcgg
     6301 caaactcacc cactacgcct acaccggctg cgacccgcgc cgcatgggcc tcggcccggt
     6361 gcgcgcgctg gatgccgtgc tgcaccacag cggcatgagg ctggaggcgg cggatgtcat
     6421 cgagatcaac gaggcctttg ccgcgcaggt gctcgccgtt ttgaaatgcc tcaaagaccc
     6481 tgcctgcgcg cgccgtgccg gactcgacgc gcctctgggc gagatcgccg acgagcagct
     6541 caatccgcgc ggtggcggca tcgccctcgg tcatcccgtc ggggccacgg gcgcgcgtct
     6601 cgtgctcacc gcgctcgacc agctccgcga gtccggcggc aagcgcgcgc tcgtctccct
     6661 ctgcatcggc ggcggccagg gcggcgcggc cttgctggag cgcgattgat ttttaccacc
     6721 ccaaaacccg actaaccctc atgaaaacgg aacttctcca atccctcgaa aaaaatcatg
     6781 aggacgcctt catcccgcgc cctcccagtc caatgatccg cgcaaaatcc ctgcggcact
     6841 tccgcctcga ctccgacgag gacggcaccg cctggatcac cttcgacatg gaaggcactg
     6901 cggccaatgt gtggaatgaa accaccctcc atgagttcga cctctgcctg gaggccgtga
     6961 gccgtgactc cacattgaag accctcgtca tccaaagcgc gaaggagcac atcttcatcg
     7021 ccggggccga cctcaaagcg gtgcgccacg ctcctgtgcg gcagctcgag acgttgatcg
     7081 gccttgggca aaaagtcttc aaccgcctcg ccgcgctgcc cttgcacaag atcgccctca
     7141 ttcatggcgc ctgcctcggc ggcggtctgg agctggccct ggcctgcgac acccgcatcg
     7201 tcagcgacag cgaccgcacg cgcctcggcc tgcccgagac acagatcggc ctggtgcccg
     7261 cctggggcgg ttccacgcgg ctgccgcgcc tccttggtct gcctgcggcc ttgcagctca
     7321 tcgtcaccgg caagctgctc aaaccctccg ccgcgaaacg cgctggcctc gccgatttca
     7381 tcgtgccgcg cgagcgcttc gaggacttcg tgcatcaccg catcgcgctc ggcgcgccaa
     7441 agcgccgcct ctctctgacc gggcgcatca gcctgctgcc aggcgcgcgg cacttcatcg
     7501 ccagccaggt gaaaaaggcc gtgctcacca aaacacgcgg cctttatcaa gctccgctgc
     7561 gtgccgtgga ggtgacgatg cgcgcctctg tcgcgccctt gccaaaggcc atggaaatcg
     7621 agcgccatgc catccaggac ctggcgctca cacccgccac ggggcatttg atcgacctct
     7681 tcttccgcaa ggaggaggcg gacaaaaagc cctggccgcg cggagtggcc ctgccggtgc
     7741 atcacgccgc cgtcgtcggc gctggtgtca tgggcgcggg catcgcgcac tggctggcct
     7801 cgcgcggtgt gcgcgtgctc atgcaggaca tcagcacgga tgccctggcg cgcggcatgg
     7861 accgcatgca tggcctgctg aaggaaggcg tcaagcgccg ggccatcagc cgcgtccaga
     7921 tgcgcgacac gctggaccgc ctcaccgccg agcacacgcg tgtgcccttg acgcatgagc
     7981 agatcatcat cgaggccgcc acagaggaca tggccttgaa gaaaaaaatc ttcgccgacc
     8041 tcgccgcgcg ctgcggtcct gacaccgtgc tcgccacgaa cacctcagcc ttgtccgtga
     8101 cggaactcgc cgagacactg ccgcaccctg agcgcgtcat cggcctgcat tttttcaatc
     8161 cagtgcatcg catgccgctg gtggagatca tcactacgcc gcacaccagc gatgatgtca
     8221 tcgccaccgc caccgccttt gtgcaaaagc tggggaagac ccccgtcgtg gtcagggaca
     8281 gccccggctt cgtcgtcaac cgcgtgctca tgccctacct catggaggcc gtgcggcttt
     8341 atgagagcgg catcccggcg gagacgatag acgaggccat gctcgagttc ggcatgccca
     8401 tgggaccgct gcgtctgctg gatgagatcg ggctcgatgt cgccgctcat gtgggccgca
     8461 ccctcgtcgc cgccttcccg gaccacctcg ccgccagtga cctgctcgac cgcatggtca
     8521 gcacgggcag gttgggcaaa aaatccggcg aaggcttcta caagcatgga gccagggtga
     8581 agcgcaaaac cgtgaagcac gacgcctccg agctggagca tgtgcagcac cgcctggccc
     8641 tgctgctcgc caacgaagcc gcgcgctgcg cccgggaaaa actcacccaa ccccccgagt
     8701 ccatagacct cgccatgatc ctcggcaccg gttaccctcc cttcctcggc ggtccactgg
     8761 cctggctgcg ggagctcgga gcagagaatg ccaagcttga actgcaaatg ctcgccgcca
     8821 ccacacccca gccgaacgcc ttcgagcctg catgagaaaa agcccaccgc gcaagcatca
     8881 aagcgcctct ctacgacatc gccgccttcg aacccaatag gccctctggc aggtcatcaa
     8941 acaatgaatc tccgccgatg acaatgaaat cagcatccat ggcagcacgc ccaccactgt
     9001 aacaaatccc ctgaatgccc caagaagttc cactcgatga ggaccgcatc ctcgtcctcg
     9061 tgccgaatga acccgattga tcctcaacaa ccccacaata cccgcaagcg aaaaagtgct
     9121 tggctcaacg gaagagaaag ttaagcccga atttcgaagt taggcgacgt ttttgaggtt
     9181 gtttagaggc ggcgtggcaa caggaggttc caacttcggc gaagttcgtt ttcgccacgc
     9241 caagaggttg cccatcaagg gctggtgctt ctcagcgcca gcccagcttt gcgcgatgtg
     9301 gagcaccggc tgccgtcccc gcttgccgag gatggctcca cccatgaaca ccgtgctgcg
     9361 cagtgtcgcg gggccttgat agtgcttctc actgcgcgtg ccggtggctt gttggtagag
     9421 gctcacgagg ttgaacgcga agatcacgca caaaaacgcc gcctctgtgg cgtaggaccg
     9481 cctcatgcag aatccgtcag cggccaggtc gttcttgagc tcttcgattc gctgctcgat
     9541 gcaggcactt tggttgtaat cgcgacacag cgtcacgtgg tcttcgtcgc ggttggtcac
     9601 caaaatgcgg taggtgtggc caggcacccg gatggaccca aaaataatgc ttgtgcaaaa
     9661 attacccgtt ccttagcctt cacctgcccc cggataaagc ca
//
LOCUS       DAOMYJ010000196         9649 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Prosthecobacter sp. isolate AMR_MDS_5629
            NODE_2353_length_9649_cov_21.806546, whole genome shotgun sequence.
ACCESSION   DAOMYJ010000196 DAOMYJ010000000
VERSION     DAOMYJ010000196.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867850
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prosthecobacter sp. (wastewater metagenome)
  ORGANISM  Prosthecobacter sp.
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Verrucomicrobiaceae; Prosthecobacter.
REFERENCE   1  (bases 1 to 9649)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9649)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.30
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:34:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,742
            CDSs (total)                      :: 4,684
            Genes (coding)                    :: 4,676
            CDSs (with protein)               :: 4,676
            Genes (RNA)                       :: 58
            tRNAs                             :: 55
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9649
                     /organism="Prosthecobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2353_length_9649_cov_21.806546"
                     /isolate="AMR_MDS_5629"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1965333"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..363
                     /locus_tag="PLP58_17070"
     CDS             <1..363
                     /locus_tag="PLP58_17070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ10293.1"
                     /translation="ALPGGGLTVPKTVVAAEAQGINTNTLTAAQQQVLAAPAVAKIKT
                     VVKEQGFVILDAGSKKGLAKGQKFNVRRDSAILARIAIADTIEEDEAVADIDLASIPA
                     GVSIEPGDEIIEPVGSSR"
     gene            367..1101
                     /locus_tag="PLP58_17075"
     CDS             367..1101
                     /locus_tag="PLP58_17075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012420857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlyA family RNA methyltransferase"
                     /protein_id="HRJ10294.1"
                     /translation="MARIRLDLALVQQGHCPSREQARRLIMAGEVLVADEVISKPGWL
                     VRQDAPLRVKHPPRFVSRGGLKLEGALDHFGLDVTGLTAMDIGASTGGFTDCLLQRGV
                     VKVYAFDVGTNQMVWKLRSDPRVVCRENFNVRHLRPEDVAEPVDLIVADVSFISLTLV
                     LPGPIEALKSGGQGLVLVKPQFELSREEIGPGGIVREPDLHAKACARIRSFIESQPGL
                     RWLGLTESTIQGADGNREFLAWFAKA"
     gene            1141..1875
                     /locus_tag="PLP58_17080"
     CDS             1141..1875
                     /locus_tag="PLP58_17080"
                     /inference="COORDINATES: protein motif:HMM:NF014630.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-type zinc ribbon domain-containing protein"
                     /protein_id="HRJ10295.1"
                     /translation="MMPVCAIIRTMLPQITQLLQLQERDQRIRALQKELKDIPKSEAL
                     AKTQLAGDLAAVEAASQKNKEIEVRIKSVELDIQTRQNSIKRLQDQQFETRKNEEFQA
                     LGVEVQRYQREVTALEDKELEFMEELEAGKAALKEAQGRLAETQARVDEELKSLAARA
                     AGVQKRLDDELAERAGLVAPIEPGPLDLYTRLFAKKPDAAVVALENGICGGCHMKVVI
                     GTIQALRQSEGLTQCESCGRILYLVE"
     gene            complement(1883..2275)
                     /locus_tag="PLP58_17085"
     CDS             complement(1883..2275)
                     /locus_tag="PLP58_17085"
                     /inference="COORDINATES: protein motif:HMM:NF013970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HRJ10296.1"
                     /translation="MIHLDTNLLVDLATAGSPHVAVIRQWLRDGEQLAVSAVAWSEFL
                     NGPHSKQQKDAIHAVLEGRILDFGEKAAEQASRLFHYTGRRRGSHSDCMIAAAAMFCG
                     VRVATRNVKDFERFVPHGLQLLPVETLA"
     gene            complement(2278..2505)
                     /locus_tag="PLP58_17090"
     CDS             complement(2278..2505)
                     /locus_tag="PLP58_17090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ10297.1"
                     /translation="MLDALAKRWQVPKAEVMRRAIKRLKEEEDLKDQCPKPLEALDWL
                     QNGGGLTVQEADAFKEDLRAEREAKRYWWEA"
     gene            2688..6389
                     /locus_tag="PLP58_17095"
     CDS             2688..6389
                     /locus_tag="PLP58_17095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009961959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1553 domain-containing protein"
                     /protein_id="HRJ10298.1"
                     /translation="MKRALAISLLPGFLWAANDTKDAAQNDSTPIPHVAPLVKWTFEN
                     QEPGSLHGKVEIKAEGPQKPMYPGFAAGNKAAVFTGKDSFIQIKESDLPEKNLRFGNG
                     DTITLEAWINTEQMGNGSFVYLIGKGRNKNKAFTSENQNWALRLKGEGGEARPCFLFR
                     SRDKDGKENYHRWVSKEGFVPGSGWHHVAVSYSFGKPKTIAAWVDGKKAPGGVWDLGG
                     ETTEPPVSDADDIMIGTGNGGGAGNTLNGALDEVAVYRELVPEVTLAQRFQFIPPPPP
                     IDAKKLPTGKVLVQVCEEGVPAKNSWPAYPPQPVETYTEDVFGIFEVPHKYVDTGVRG
                     DRPNPYLLRAAAVVIVPAGKHRLLVRGRNATHLSVDGVMLVDLPIAKADGGGHGRVSE
                     QDEYLDLGSDFRFAPPGSQEKWIEFESEGGEHLFVIEQMIGGVIGSSKRRPETGEMVV
                     AISLEGSESWSLLSPGKRQVPYTDEGWAAYETERRVWLTKFNAERRAKARAQHDAYWT
                     KRREAASKWLASTPAVKVPAPVKDLPANNAVDHFLNAKIAAVSAQYSAAKKDGVHFYK
                     EVLPILEQNCFSCHQGGKTKGDLRLDTLSGALKGGESDGPAIAPGHPAKSSLIARVST
                     DDEDYIMPPKGSPLTKEQIAVLERWISEGANWPELNVDRIEVTPLSDDLAFLRRVTLD
                     TVGVVPTLEEIAAFQKDTRKDKRARVIDRLLADPRWADRWMGYWQDVLAENPNILNPT
                     LNNTGPFRWYLHEALSDDRPMDLFVTELLRMGGSERFGGPAGFGTASGNDVPMAAKGA
                     IISTAFLGVEMKCARCHDAPAHESTQQDLFELAALLETKSLDVPKTSSVPMDKLHEGG
                     RKPLIQVTLQPGSKVEPKWPFEDFVPHSVGESLAEDPKNTRDVLAALVTAPQNERFAQ
                     VIVNRLWQQYMGRGIVQPVEDWEKGKPTHPELLAWLAREFVRGGYSLKNLSRLILNSH
                     AYQRATDSALSGPSPVFAAPAPRRLAAEQIVDSLFAATGKAMKTEEVSLDIDGRRDLK
                     NSISLGQPNRAWMFASTSNERDRPSLSLPRIQAVCDVLEAFGWRATRQDPASSRDTDP
                     NALQPAILGNGTVAVWLTRLSEDHGITQLALKDQPAEQLVDQLFLRLLTRLPTAEERQ
                     LYTDHLRAGYDQRRLEYKPEKPDQRYPAKYVSWTNHLDPVANALRVEEEAAARRGDTP
                     SPRLDNDWRLRMEDVLWAVLNAPEWVFAP"
     gene            6471..6707
                     /locus_tag="PLP58_17100"
     CDS             6471..6707
                     /locus_tag="PLP58_17100"
                     /inference="COORDINATES: protein motif:HMM:TIGR02574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="addiction module protein"
                     /protein_id="HRJ10299.1"
                     /translation="MSTTAEALLAPLLALPEQDRLMIADRLHESLHDAPPNDDLSDEM
                     KATLDRRWAEIESGKVECIPHEVVMEKLRARYAV"
     gene            6697..7008
                     /locus_tag="PLP58_17105"
     CDS             6697..7008
                     /locus_tag="PLP58_17105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ10300.1"
                     /translation="MRYEVVTHPEAFAELQEALDWLDERSLWAGKRLSREYDEHISLI
                     RDTPASLRKIHGDYRRVNLRRFSYHVIYRIRQDHIFVIAFAHDKRHPDYWKARISDDQ
                     D"
     gene            7046..8314
                     /locus_tag="PLP58_17110"
     CDS             7046..8314
                     /locus_tag="PLP58_17110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009961958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1501 domain-containing protein"
                     /protein_id="HRJ10301.1"
                     /translation="MKRRRLLQTAALAPLAASGLRASNVVAMPKGKAEHCIFIWLGGG
                     MAHMDTFDPKALGDPKASPKKAGAAYPAIETSVPGVQVCEHLPKTARLMEHITVMRTI
                     HHNIINEHAIATNFVHTGRQISGDTTYPSLGSIVAHQRGAANPKVPAYMLIGYPNPSR
                     GPGFLGAKHGNIYLTDTQSGPAGFTRPADVTAQRVSAREKLLAPLQERVARDSMLADY
                     EAAQREALRLAGPEFMRNFKLDGEPADLRNAYGSEFGQRCLLARRLVQDGVRFIEVSH
                     NLAFVNGTGWDTHNDGQEKQHYLIKDLDDALSTLITDLKNHGLLDKTLIALGTEFGRP
                     PEFDGGGGRGHQCSTFSLLLAGGGLNHCGAYGVTDDLAKQIVENPVSVPDYHATIHAA
                     LGINPAHELIAGTRPVPITDGGKPVAKLFG"
     gene            8428..>9649
                     /locus_tag="PLP58_17115"
     CDS             8428..>9649
                     /locus_tag="PLP58_17115"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013434.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase/oxidase"
                     /protein_id="HRJ10302.1"
                     /translation="MTETLTSDRRPGLRFLESEPLDVLVIGGGIVGSSVARDAAMRGL
                     RIGLIEQRDFAFGTSGRSSRLLHGGIRYLAQGRIGLVREASVEKTLLHRIAPHLAAPL
                     PFVFPTYRHNRAWKLWQLMIGVKLYDLLCGGRNLGASSWHDAEDTQRLVPGLAADGLK
                     GAVRYFDGFTNDARLVLDSLRSAAAHGAVVLNRVRFVNARRDQLWQCTVEDTHAGRTL
                     EIQARTVVNATGPWAHGLPHSAVKLRLTKGIHLVVEHARLPVTDTVVMTEGTRILFAI
                     PWGARTILGTTDTDYNGPPENVRTDAADIAYVLAIANQHFPAAHLAEADVISTWAGLR
                     PLIADPKGRPSDISRNHEIKSPEPDWWDVAGGKLTTCRLMAEQTLDKLTRAANLRALP
                     CSTADEPLLADPGFS"
BASE COUNT         1876 a         3483 c         2733 g         1557 t
ORIGIN      
        1 gccctgcctg gcggcggtct cacggtgccc aagaccgtgg tcgcggcgga ggcgcagggc
       61 atcaatacaa acacactcac cgccgcccag cagcaggtgc tggccgcgcc cgccgtggcg
      121 aagatcaaga ccgtggtcaa ggagcagggc ttcgtgatcc tggacgcggg ttcgaaaaag
      181 ggcctcgcca aaggccagaa attcaacgtc cgcagggata gcgccattct cgccaggatc
      241 gccatcgcag acaccatcga ggaggacgag gccgttgcgg acattgacct cgcctccatt
      301 cctgcggggg tctccatcga gcctggagac gagatcatcg aacccgtcgg cagcagccgc
      361 tgagacatgg cccgcatccg gctggacctc gccctcgtgc agcaagggca ctgcccctcc
      421 cgcgagcagg ccaggcgtct catcatggcg ggcgaggtgc tcgtggccga cgaggtcatc
      481 agcaaacccg gctggctcgt gcggcaggac gcgcctctgc gcgtgaaaca cccgccgcgt
      541 tttgtcagcc gcggcggttt gaagctggag ggcgcgctcg accacttcgg cctcgatgtc
      601 accggactca ctgccatgga catcggcgcc tcaacgggcg gcttcactga ctgcctgctc
      661 cagcgaggtg tcgtaaaagt ttatgctttc gatgtcggca cgaaccagat ggtctggaag
      721 ctgcgcagcg acccgcgcgt ggtctgccgg gagaatttta acgtccgcca tttgcgcccg
      781 gaggacgtgg ccgaacccgt ggacctcatc gtcgcggatg tctccttcat ctccctcacg
      841 ctcgtcctgc ccggccccat tgaggcgctg aaatccggcg gccagggcct tgtgctcgtg
      901 aagccgcagt tcgagctcag ccgggaggaa atcggccccg gcggcattgt gcgcgagccc
      961 gatctgcacg cgaaagcctg cgcgcgcatc cgctccttca ttgagtcgca acccggcctg
     1021 cgctggctcg gtctcaccga gtccaccatc cagggcgctg acggcaaccg cgagttcctc
     1081 gcctggttcg ccaaagcgtg agcgcttttt cccagaaagc ccggctttga cgcttgcgcc
     1141 atgatgccgg tctgcgccat catccgcaca atgcttcctc aaatcaccca actgctccag
     1201 ctccaagaac gcgaccagcg catccgcgcg ctgcagaagg aactcaagga catcccgaag
     1261 agcgaggctt tggcaaaaac ccagcttgcc ggcgacctcg ccgccgttga ggcggcaagc
     1321 caaaaaaaca aagagatcga ggtgaggatc aaaagtgtcg aactcgacat ccagacgcgt
     1381 caaaacagca tcaagcgcct ccaggaccag cagttcgaga cacggaaaaa cgaggaattt
     1441 caagcgctcg gtgtcgaagt gcagcgctac cagcgggaag tgaccgctct cgaggacaag
     1501 gaactcgagt tcatggagga gttggaggcg ggaaaagccg cgctgaagga ggcccagggc
     1561 aggctggcag aaactcaggc gcgcgtggat gaggagttga aatccctggc agcgcgcgca
     1621 gccggagtgc aaaagcgcct cgacgatgag ctggccgagc gtgccggact cgtcgccccc
     1681 attgagccgg gaccgctcga cctctacacg cgtctctttg caaaaaagcc cgacgcggct
     1741 gttgtcgccc ttgagaatgg catctgcgga ggctgccaca tgaaggtcgt catcggcacc
     1801 atccaggccc tgcgccagtc cgagggactc acccaatgcg agagctgcgg acgcattctt
     1861 tatctggtgg agtagtcctg gctcaagcga gcgtctccac cggcagaagt tgcagcccgt
     1921 gcggcacgaa gcgctcgaag tccttcacat ttcgcgtcgc cacgcgcacg ccgcagaaca
     1981 tggccgccgc ggcgatcatg cagtccgagt gcgagccgcg ccggcggccc gtgtaatgaa
     2041 aaagacgcga ggcctgctcg gcggcctttt cgccgaagtc caggatgcgg ccctccagca
     2101 cggcgtgaat ggcgtccttt tgttgcttcg agtgcggccc gttcagaaat tccgaccatg
     2161 ccaccgccga cacggcgagc tgctcgccat cccggagcca ttggcggatc accgccacat
     2221 gcggcgagcc cgccgtggcg agatccacga gcaaattcgt gtccaggtgg atcatgctca
     2281 ggcctcccac cagtagcgtt tggcctcgcg ctcggcgcgc aggtcctcct tgaaggcgtc
     2341 agcctcctgg acggtcagac cgcctccgtt ttggagccag tcgagggctt cgaggggttt
     2401 ggggcattgg tctttcaagt cttcctcctc cttcaatcgt ttgatggccc ggcgcatgac
     2461 ctcggctttg ggcacctgcc agcgtttggc gagtgcgtcc aacatgcgga gggtgaaccc
     2521 gtccagtgac atgcttgtgc gtgtgagtgc atacgacatg tggtaacatg ttacagcacg
     2581 tcttcccttc aactgttttt ttcatttttt cccatccccc gaaagtgggg ggggctggat
     2641 tcatgaaaag gcggttcatg acacgttagt tcccttgtca tccgaccatg aaacgagcac
     2701 tcgccatttc cctcctcccc ggattcctct gggccgccaa cgacaccaag gacgcggcgc
     2761 agaatgactc cacgcccatc ccgcatgtcg cacctctggt gaagtggacc tttgaaaacc
     2821 aagaacccgg ctcgctgcat ggcaaggttg agatcaaggc cgaggggccg caaaagccca
     2881 tgtatcccgg ctttgccgcc ggcaacaaag ccgccgtttt cactggcaag gacagcttta
     2941 tccagatcaa ggaaagtgat ttgccggaaa aaaatctgcg cttcggcaat ggcgacacca
     3001 tcaccctcga agcctggatc aacacagagc agatgggcaa cggcagcttc gtttatctta
     3061 tcggcaaagg gcggaacaaa aacaaagcct tcacctccga gaatcaaaac tgggcgctcc
     3121 gcctcaaagg cgagggcggt gaggcacggc cctgcttcct tttccgcagc cgcgacaagg
     3181 acggcaagga aaactaccac cgatgggtgt ccaaggaggg ctttgtcccc ggctccggct
     3241 ggcatcatgt ggccgtgagc tactccttcg gcaaacccaa gaccatcgcc gcctgggtgg
     3301 atggcaaaaa agctccgggc ggcgtctggg atctgggagg tgagaccacc gaaccacctg
     3361 tgagtgacgc ggatgacatc atgatcggca ccggcaatgg cggcggcgct ggcaacacgc
     3421 tcaatggagc gctcgacgag gtggccgtgt atcgcgaact cgtccccgag gtcaccctgg
     3481 cgcagcgctt ccagttcatc ccgcccccgc cgcccattga cgccaaaaag ttgccaacgg
     3541 gcaaagtgct cgtgcaagtc tgcgaggagg gcgtgcccgc gaaaaactcc tggcccgcct
     3601 accctccaca gcccgtcgag acctacaccg aggatgtctt cggcatcttt gaggtgccgc
     3661 acaaatatgt ggacaccggc gtgcgcggcg accggccaaa cccttatctc ctgcgcgccg
     3721 cagccgtcgt gatagtgccc gccggcaagc accgtctgct cgtgcgaggc cgcaacgcca
     3781 cccatctgag cgtggacggt gtgatgctag tggacctgcc tattgccaag gccgatggcg
     3841 gcggccatgg ccgcgtgtct gagcaggacg agtatctcga tctcggctcc gattttcgtt
     3901 ttgcgcctcc tggatcgcag gaaaaatgga tcgagtttga gagcgagggc ggcgagcatc
     3961 tctttgtcat cgagcagatg atcggtggcg tcatcggcag ctcgaagcgc cgcccggaga
     4021 ccggcgagat ggtcgtggcc atttccctgg aaggcagcga aagctggagc ctgctcagcc
     4081 ctggcaaacg ccaggtgccc tacaccgacg aaggctgggc cgcctatgaa accgagcgcc
     4141 gcgtctggct cacgaagttc aatgccgagc gccgcgccaa ggcccgcgcg caacatgatg
     4201 cctactggac caagcgccga gaggccgcct caaaatggct cgcctccact ccagcagtga
     4261 aagtgcccgc gcctgtcaaa gacctgccag ccaacaatgc ggtggaccat ttcctcaacg
     4321 ccaaaatcgc cgccgtcagc gcgcaatact ccgccgccaa aaaggacggc gtgcatttct
     4381 acaaggaggt gctgcccatc ctggagcaga actgcttcag ttgccatcaa ggcggcaaaa
     4441 ccaagggcga cctccgcctg gacaccctct ccggcgcgct caaaggcggc gagtcagacg
     4501 gtccggccat cgcgcctggc caccccgcca aaagctccct catcgcccgc gtcagcacgg
     4561 atgacgagga ctacatcatg cctcccaaag gcagcccgct caccaaggag cagatcgccg
     4621 tgctggagcg ctggatcagc gagggcgcga actggcccga gctcaacgtg gaccgcatcg
     4681 aggtcacgcc cctcagcgat gacctcgcct tcctccgccg tgtcaccctc gacaccgtcg
     4741 gcgtcgtgcc cacgctggag gagatcgccg ccttccaaaa ggacacccgc aaagacaagc
     4801 gcgccagagt gattgaccgc ctgcttgccg acccgcgctg ggccgaccgc tggatgggtt
     4861 actggcagga cgtgctcgcc gaaaacccga acatcctcaa tcccaccctc aacaacaccg
     4921 gccccttccg ctggtatctg catgaggcgc tcagcgacga ccgccccatg gacctctttg
     4981 tcaccgagtt gctgcgcatg ggcggcagcg agcgcttcgg cggcccggcc ggctttggca
     5041 ccgcctccgg caacgacgtg cccatggccg ccaaaggcgc catcatcagc accgccttcc
     5101 tcggcgtgga aatgaagtgc gcccgctgcc acgacgcccc cgcgcacgag agcacgcagc
     5161 aggacctctt cgaactcgcg gcgctgctcg aaaccaagtc cctcgacgtg cccaaaacca
     5221 gcagcgtgcc catggacaag ctgcacgaag gcggccgcaa gccgctcatc caggtcaccc
     5281 tccagcccgg cagcaaagtc gagcccaagt ggcccttcga ggacttcgtg ccgcacagcg
     5341 tcggtgaaag cctcgccgag gatccgaaga acacgcgcga cgtgctcgcc gcgctcgtca
     5401 ctgcgccgca aaacgaacgc ttcgcccagg tcatcgtcaa ccgcctctgg cagcaataca
     5461 tgggccgcgg catcgtccag cccgtcgagg actgggaaaa aggcaagccc acccaccccg
     5521 aactgctcgc ctggctggcg cgcgagtttg tgcgcggcgg ttacagcctg aaaaacctca
     5581 gccgcctcat cctcaactcc cacgcctatc agcgcgccac cgactccgcc ctcagcggcc
     5641 ccagccccgt cttcgccgcg cccgcgccgc gccggctcgc cgccgagcag atcgtggact
     5701 ccctctttgc cgccactggc aaggccatga agaccgagga ggtcagcctc gacattgacg
     5761 gccgccgcga cctgaagaac tccatctcgt taggccagcc gaaccgcgcc tggatgttcg
     5821 ccagcaccag caacgagcgc gaccgcccca gcctcagcct gccgcgcatc caggccgtgt
     5881 gcgacgtgct cgaagccttc ggctggcgcg ccacccgcca ggaccccgcc agcagccgcg
     5941 acacggatcc caacgccctc cagcccgcca tcctcggcaa cggcaccgtc gccgtctggc
     6001 tcacccgcct cagcgaggac cacggcatca cccaactcgc gctcaaagac cagccggcgg
     6061 agcagctcgt ggaccagctt ttccttcgtc tcctcacccg cctgcccact gctgaggagc
     6121 gccagcttta caccgaccat ctgcgtgccg gttacgatca gcgccgcctc gaatacaaac
     6181 ccgaaaaacc cgaccagcgt taccccgcca aatacgtctc ctggaccaac cacctcgacc
     6241 ccgtcgccaa cgccctgcgt gttgaagaag aagccgccgc acgccgtggc gacaccccca
     6301 gcccccgtct cgacaacgac tggcgcctgc gcatggaaga tgtcctctgg gccgtgctca
     6361 acgctcccga gtgggtcttc gccccgtagc agcgacgccc ccgccgcttc cccgatgaca
     6421 gcacgccctt ttcgctaccg cttttcctcg aaaccgttat tctccaagtc atgagcacca
     6481 ccgccgaagc cctcctcgcc cccctgctcg ccctgcccga gcaggaccgc ctcatgattg
     6541 ccgaccggct ccatgaaagc ctgcatgacg ccccgcccaa cgacgacctc agcgatgaga
     6601 tgaaagccac gctggatcgc cgctgggcag agatcgagag cggaaaggtc gaatgcattc
     6661 ctcacgaagt ggtcatggaa aaactgcgcg cccgttatgc ggtatgaagt cgtcacccac
     6721 cctgaagcgt ttgcggaact ccaggaggct ctcgactggc ttgatgaacg cagtctgtgg
     6781 gctggcaaac gcctttcccg tgaatacgac gaacacattt ccctcatccg ggacacaccc
     6841 gcatccttgc gcaaaattca tggcgactac cgccgtgtga acctccgccg cttctcctac
     6901 cacgtcatct accgaatccg tcaggatcac atcttcgtca tcgccttcgc tcacgacaaa
     6961 cgccaccccg actactggaa agcccgcatc agcgacgatc aagattagcc gcgaaccacg
     7021 aacccccaac tccgacccca ttcccatgaa acgccgccgc ctccttcaaa ccgccgccct
     7081 tgccccgctc gccgccagcg gcttgcgcgc ctccaacgtc gtcgccatgc ccaagggcaa
     7141 ggccgagcac tgcatcttca tctggctcgg cggcggcatg gcgcacatgg acacctttga
     7201 tccgaaggcg ctcggcgacc cgaaggccag cccgaagaaa gccggcgccg cctaccccgc
     7261 catcgagacc tccgttcctg gtgtccaggt ctgcgagcac ttgcccaaga ccgcccgcct
     7321 gatggagcac atcaccgtga tgcgcaccat ccatcacaac atcatcaacg agcacgccat
     7381 cgccacgaac ttcgtccaca ccggacgcca gatcagcggc gacaccacct acccctccct
     7441 cggctccatc gtcgcccacc agcgcggcgc ggccaatccg aaagtgccag cctacatgct
     7501 catcggctac ccgaacccca gccgcggccc cggctttctc ggcgccaaac acggcaacat
     7561 ctacctcacc gacacccaga gcggccccgc cggtttcacc cgccccgccg atgtcaccgc
     7621 gcagcgcgtc agcgcgcgtg aaaagctcct tgccccgctg caggagcgcg tcgcgcgcga
     7681 ctccatgctc gccgattacg aagccgccca gcgcgaggcc ctgcgcctcg ccggtcccga
     7741 gttcatgcgc aacttcaagc tcgacgggga acccgccgac ctgcgcaacg cctacggcag
     7801 cgagttcggc cagcgctgtc tgctggcccg ccgcctcgtt caggacggcg tgcgcttcat
     7861 cgaagtctcc cacaacctcg ccttcgtcaa cggcaccggc tgggacaccc acaatgacgg
     7921 ccaggaaaag cagcactacc tcatcaaaga cctcgacgac gccctctcca ccctcatcac
     7981 cgacctcaaa aaccacggcc tgctcgacaa aaccctcatc gccctcggca ccgagtttgg
     8041 ccgtccgccc gagttcgacg gcggcggcgg ccgcggccac cagtgcagca ccttcagcct
     8101 ccttcttgcc ggtggtgggc tgaaccactg cggcgcttat ggcgtcaccg acgacctcgc
     8161 caaacagatc gtcgaaaacc ccgtctccgt gcccgactac cacgccacca tccacgccgc
     8221 cctcggcatc aaccccgcgc acgagctcat cgccggcact cgtccggtgc ccatcaccga
     8281 cggcggcaaa cctgtggcga aactcttcgg ttgagccgga cccgacttgc aatcccaatg
     8341 ccccgccatc gcattggctt ggcacgtcgt cacacggcat tcacgaaacg attgcatctc
     8401 ccccgggtcc gcagttgcat gggcctcatg actgaaaccc tcacgagcga ccgtcggccg
     8461 ggtctgcgtt ttctggaaag cgaaccgctc gacgtgctcg tcatcggcgg cggcatcgtc
     8521 ggctccagtg tcgcgcggga tgccgccatg cgcggcctgc gcatcggact catcgagcag
     8581 cgcgactttg ccttcggcac cagcgggcgc tccagccggc tcctgcacgg cggcatccgc
     8641 tacctggcgc agggccgcat tggcctggtg cgtgaggcca gtgtggaaaa gaccctcctg
     8701 caccgcatcg ccccgcatct ggccgcgccg ctgccctttg tcttccccac ctaccgccac
     8761 aaccgcgcgt ggaagctctg gcagctcatg atcggcgtga aactgtacga cctgctctgc
     8821 ggcggacgca acttgggtgc ctcgagctgg cacgatgcgg aggacaccca gcgtctcgtg
     8881 cccggcctcg ccgccgatgg attgaaaggc gcggtgcgtt acttcgacgg cttcaccaat
     8941 gacgcgcgcc ttgtgctcga ctcgctccgc tccgccgccg cgcacggcgc cgtcgttctt
     9001 aatcgcgtgc gttttgtcaa tgcccggcgg gatcagctct ggcaatgcac cgtcgaggac
     9061 acccacgccg gccgcacctt ggaaatccag gcgcgcaccg tcgtcaatgc caccggcccc
     9121 tgggcgcacg gcctgccgca cagcgcggtc aaactgcgtc tcaccaaagg catccacctc
     9181 gtcgtggagc acgcgcgttt gccggtcact gacaccgtgg tcatgaccga aggcacgcgc
     9241 atcctcttcg ccatcccctg gggcgcgcgc accattctcg gcaccaccga caccgactac
     9301 aacggccccc cggaaaacgt gcgcactgat gccgcggaca tcgcctatgt gctcgcaatc
     9361 gccaaccagc atttccccgc cgcgcatctt gccgaagcgg atgtcatcag cacctgggcc
     9421 ggactgcgcc cgctcattgc cgaccccaaa ggccggcctt ccgacatctc ccgcaatcac
     9481 gaaatcaaaa gccccgagcc ggactggtgg gacgtggccg gcggcaagct caccacctgc
     9541 cgcctcatgg ccgagcagac gctcgacaaa ctcacccgcg ccgccaacct gcgcgccctg
     9601 ccctgctcca ccgccgatga gccgctgctg gcggaccccg gtttcagcg
//
LOCUS       DAOMYJ010000197         9614 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Prosthecobacter sp. isolate AMR_MDS_5629
            NODE_2364_length_9614_cov_16.258814, whole genome shotgun sequence.
ACCESSION   DAOMYJ010000197 DAOMYJ010000000
VERSION     DAOMYJ010000197.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867850
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prosthecobacter sp. (wastewater metagenome)
  ORGANISM  Prosthecobacter sp.
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Verrucomicrobiaceae; Prosthecobacter.
REFERENCE   1  (bases 1 to 9614)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9614)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.30
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:34:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,742
            CDSs (total)                      :: 4,684
            Genes (coding)                    :: 4,676
            CDSs (with protein)               :: 4,676
            Genes (RNA)                       :: 58
            tRNAs                             :: 55
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9614
                     /organism="Prosthecobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2364_length_9614_cov_16.258814"
                     /isolate="AMR_MDS_5629"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1965333"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..371
                     /locus_tag="PLP58_17120"
     CDS             <1..371
                     /locus_tag="PLP58_17120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ10303.1"
                     /translation="PRAGVLTLATDNGDNAPLRLESVRASHPVVRLLFRVPDTEPARL
                     CYGNPRAAYARYDLDLVRREFEAAVKVPATLGEEEKLPGHREERGVSGKGSPWLWAAL
                     ALVVGALLWIVAGMLPRQQA"
     gene            complement(385..1527)
                     /locus_tag="PLP58_17125"
     CDS             complement(385..1527)
                     /locus_tag="PLP58_17125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009959928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HRJ10304.1"
                     /translation="MRSLILLPFLACAALAQNAAPKAKSPDISKLDPAMGGNKAAAPE
                     LDWHDVTAWGVEGRILPEQERKRWFDRLPASAEGKVTPAVWSLSRDSAGMMARFKTDS
                     ASIHVHYKVMKDKLGMPHMPATGVSGADLYARDTGGKWKWVQVTRPAAQEIKVEMIQG
                     LAPGLREYALYLPLYNGTESFQIGVPKGARFEGLAPRAKPVVFYGTSITHGACASRPG
                     MVHTAILGRWLDMPVVNLGFSGNGRMDKEVGDYLVQLDAAAYVIDCLPNMQPAQVAEK
                     CVPLVRQLRAAKPGTPIILVEDRRFTNDWITPAKHKFHTDNHAALRAAHETLVAEGVK
                     NLHYIKGDTLYGDDTEGATDASHASDLGFMRQAEAFLPVLRKALGK"
     gene            complement(1581..3191)
                     /locus_tag="PLP58_17130"
     CDS             complement(1581..3191)
                     /locus_tag="PLP58_17130"
                     /inference="COORDINATES: protein motif:HMM:TIGR03790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03790 family protein"
                     /protein_id="HRJ10305.1"
                     /translation="MSHAHDPAHPRRPVRRRGVAGFWLAAWLLAVEGLRGQEAGGFDL
                     STETVVAFNPDYAQSRELAQYYARMRGIPQERLVALECPLEDSMSRDEFERRVRRPLF
                     ETFVRKGWWELGQVTLTDPATGRDAPAMAVTRSSARVLVLMRGIPFQIRREKQNPQNS
                     QEDEAGVDSELCVLGLPRQPAAGAVPNPYFNQSARFQLFQTAPGLLLVARLDGPDADT
                     VKRMVDDSLAAEREGLRGRAVIDLAQKSGAYQEGEDWLKRSAILLRGQGIPVFLDRGE
                     SLIPDHWPLPDTALYFGWYAAHASGAVASPSFRFTPGAVACHLHSFSGAALRSPDRHW
                     AGPLLRQGAAAVMGNVFEPYLSLTVHFDILNQRLLEGFTLGEAAWNATPVLSWMNVLC
                     GDPLYRPFAKGPGSAMGEGRERDYALYQGAALRHPGEDTRALKSELAALAGSRSKPHL
                     LELTALLSASEGKPAEAADLLDHARSLYVLVPDRARALLYQARCMLDDDRPDEAARLL
                     RLVTGNPAHKDQPALLAARALLEQIPAE"
     gene            complement(3269..4336)
                     /gene="rsmH"
                     /locus_tag="PLP58_17135"
     CDS             complement(3269..4336)
                     /gene="rsmH"
                     /locus_tag="PLP58_17135"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007413439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016434 - rRNA (cytosine)
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HRJ10306.1"
                     /translation="MSEPENPAPHRRRPRYPGKNPRRFEDKYKELNPELHAETVAKVR
                     AAGKTPAGQHVPILLREVLEVLAPRPGERAVDCTLGHGGHAGALLKAVQPGGALLALD
                     QDPIEIVRAEALLRASGCGEDALLVRRMNFAGLPRALADLGWCDGADVLLADLGCSSM
                     QFDNPARGFSFKHDGPLDMRMNPLRGVSARDLLQKLPVEKLAEILEENADERHAALLA
                     RALAGRDHAGTRALAEAVRGALPGRLDEREREAAVRRVFQALRIAVNGEFEALDTFLR
                     QLPACLRPGARVAILTFHSGEDRRVKHVFRDGLRGGVFSSVNDEVIRPSPQEIRDNPR
                     ASPAKLRWAVAAGSAGALERE"
     gene            complement(4333..4677)
                     /locus_tag="PLP58_17140"
     CDS             complement(4333..4677)
                     /locus_tag="PLP58_17140"
                     /inference="COORDINATES: protein motif:HMM:NF024057.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphohydrolase"
                     /protein_id="HRJ10307.1"
                     /translation="MTIEQITERICAFRDAREWMQFHDPKEMAVAITAEAGELLQHFV
                     WQQQGQIEQRVRERMPELKDEMADVGILLFEMAHNLGINLGQAMLEKIERNEARYPVA
                     KARGSNAKYNEL"
     gene            4996..5970
                     /locus_tag="PLP58_17145"
     CDS             4996..5970
                     /locus_tag="PLP58_17145"
                     /EC_number="3.1.3.7"
                     /inference="COORDINATES: protein motif:HMM:NF013528.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008441 - 3'(2'),5'-bisphosphate
                     nucleotidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional oligoribonuclease/PAP phosphatase
                     NrnA"
                     /protein_id="HRJ10308.1"
                     /translation="MTPLSDIAAILRGAQRIGIAAHVRPDGDAVGSVLGLALSLRALG
                     KTVHALLEDGVPENLAFLPGAETILQPAGPLDLDVAVALDTANYERLGAGVVEIFGKA
                     PVLVNMDHHPTNPLYGHHNHIASDEPAAGQIVYELLAQNGLPVDEGVRQNLFAAISTD
                     TGSFQFASTTARSHEIAAEMIRAGLDIARLSQLLYHTFPLRRLELQRAMLNEMAFRAE
                     GRIASWQLTQETQQRCGVRPGDTEGLIDTLRMIDSVVSAVVFEELKDGKVRVSARSKD
                     SRLDVSAVCGQFGGGGHRMAAGARLPGPIPAAAETFLQALEHEVRRLS"
     gene            5948..6676
                     /gene="truB"
                     /locus_tag="PLP58_17150"
     CDS             5948..6676
                     /gene="truB"
                     /locus_tag="PLP58_17150"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012419800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004730 - pseudouridylate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HRJ10309.1"
                     /translation="MKSDDSLNGVLLVDKGPDMTSHDVVAVARRCLRTKKIGHCGTLD
                     PMATGMLILVIGTGTKLQDLLMSEDKEYTGSLKLGVTTSTQDREGEVLEEKPVPAFTA
                     DEIQAAFDAYKGDFYQTPPMVSAVKIDGVPLYKLARQGREVERKPRFVRVYDAQITRA
                     EPPDVDFRVVCSKGFYVRTYAHDIGQKLGCGAHLTALLRTRSGHFKFQPGNHTNFGAL
                     KEGRRDEVLAAMMSLYDVSKLRGA"
     gene            complement(6682..7437)
                     /locus_tag="PLP58_17155"
     CDS             complement(6682..7437)
                     /locus_tag="PLP58_17155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009965036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRJ10310.1"
                     /translation="MPDADPREVILEARELTREFDGVKALDRLSLRLHKGEILGLLGA
                     NGAGKTTAMNCLLGLTLPTSGELSAFGKPLHKHRIEILQRANFSSAYVSLPGNLKVWQ
                     NLLVFARIYGVRDAKNKIASLLEMLEIPHLSHHVTGRLSAGESTRVNLCKAFLNDPEL
                     LMLDEPTASLDPDIADKVRKVVRRVQAERGTSILYTSHNMRDIEEVCDRVIFLHKGRI
                     VAEGTPAQIVQKFAENSLEDVFIKIARSGDIEE"
     gene            7618..8019
                     /locus_tag="PLP58_17160"
     CDS             7618..8019
                     /locus_tag="PLP58_17160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ10311.1"
                     /translation="MAQHSQHKQDSSPACHGGGGQTIVELCRSTDSGLVFWSRQRFEI
                     GSELQIRIHRGALPGFAAKQIAEESDWVTVRGFVVECPAMRRQDGSHGFQVSLLLDAA
                     LIAPKPRRPRRARLLPRMNTRFAGLARPGLN"
     gene            8080..>9614
                     /locus_tag="PLP58_17165"
     CDS             8080..>9614
                     /locus_tag="PLP58_17165"
                     /inference="COORDINATES: protein motif:HMM:NF019350.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HRJ10312.1"
                     /translation="MSAVSPRVLILMLMASGAPSLRAADEAITTWNPLRLMAEKAGLR
                     ARIEEDAHRLAAMPEARVVAQSHGRVGFHGRAAAPAWIVLDLGKEAAPDEVVLLPARV
                     SDPAPDDPVEGFPPELKCLMSMTVEEGSFQVMNEWAEPAGGAGARLAALRLRNPRGLS
                     GRFLRIEITGGRPRARGMFFSLGEVWALKDGVNLALGAPVESSASIDNPPRWTAANLT
                     DGFIWCGSTTGTEKARKNGFHSRIESSPDAVPKWVEVVLDEAMPVDEIRLVPARPADF
                     ADVTGFGFPPEFQVLAWADDSPDEKPVVVFDTGGAGFANPGDATVCLPAHGVKTRRVR
                     VVAGRLWRRTSDYIFALAEMQVMSAGRNAALGREVRFSDIVESASWTPEALVDGFGSQ
                     RALLDAARWLDEMDARAALEAELLPARAGLAVLELASQRQLLLAVEILVIAMLVVLAA
                     AAILLVHQRRARAELRARIARDLHDEIGSQLSHLALLAETGGADADAALRAIKSGARE
                     TQQA"
BASE COUNT         1502 a         3094 c         3367 g         1651 t
ORIGIN      
        1 ccccgcgcgc gggtgtcttg acgctcgcca ccgacaacgg tgacaacgcg ccgctgcggc
       61 tcgaaagcgt gcgcgcctcc caccccgtgg tcaggctgct cttccgcgtg cctgacacgg
      121 agcccgcgcg gctttgctac ggcaacccgc gcgcggcata tgcgcgctat gatctcgatc
      181 tcgtccggcg cgagtttgag gccgctgtca aggtccccgc cacgctcggt gaggaggaga
      241 agctccccgg tcaccgcgag gagcgcggcg tcagcggcaa aggctccccc tggctctggg
      301 ccgcgcttgc actcgtggtc ggagcgttgc tgtggatcgt ggcggggatg ctgccgagac
      361 agcaggcgtg agaccggcgt gcggtcactt cccgagcgcc ttgcgcagca ccggtaggaa
      421 agcctcggcc tggcgcatga agccgaggtc gctggcgtgg gaggcgtccg tggcgccttc
      481 ggtgtcgtcg ccgtaaagcg tgtccccctt gatgtaatgg aggtttttta ctccctcggc
      541 cacgagggtc tcatgcgcgg cgcgcagggc ggcgtggttg tcggtgtgga atttgtgctt
      601 cgccggggtg atccagtcgt tggtgaaacg gcggtcctcg acgaggatga tgggcgttcc
      661 gggcttggcg gcgcgcagtt gcctgaccag cggcacacat ttttccgcga cctgcgcggg
      721 ctgcatgttc ggcaggcagt caatcacata agcggcggca tccagttgta cgagatagtc
      781 gcccacttcc ttgtccatgc ggccgtttcc tgagaagcct agattcacca ctggcatgtc
      841 gagccagcgg ccaagaatgg cggtatggac catgccgggg cggcttgcgc aggcgccgtg
      901 ggtgatgctg gtgccgtaaa agaccaccgg cttcgcgcgc ggtgccaggc cctcgaatct
      961 ggcccccttc ggcacgccga tttggaagga ctcggtgccg ttgtaaagcg gcagatagag
     1021 cgcgtattcg cgcaggccgg gcgccaggcc ctgaatcatc tccaccttga tctcctgggc
     1081 ggcgggccgc gtcacctgca cccacttcca tttgccgccg gtgtcgcgcg cgtagaggtc
     1141 cgcgccgctg acacccgtgg ccggcatgtg cggcatgccg agtttgtcct tcatcacttt
     1201 gtaatgcaca tggatgctcg cgctgtcggt cttgaagcgc gccatcatgc ccgcgctgtc
     1261 gcggctcagg ctccacacgg ccggggtgac tttgccctca gcgctggcgg gcaggcggtc
     1321 aaaccagcgc tttcgttcct gctcgggcag gatgcggcct tccacgcccc aggcggtcac
     1381 gtcatgccag tccagctcgg gcgcggcggc tttgttgccg cccatcgcgg ggtcgagttt
     1441 ggagatgtcc gggcttttgg ctttcggcgc ggcgttttgc gccagggcgg cgcaggcgag
     1501 gaagggcagg aggatgaggg aacgcatgtg ggtgggttgg aaaggaaaaa gcaaacgctc
     1561 cgggaagtcc gcatccatcg tcactcggcg ggaatctgct ccagcaaagc gcgcgccgcg
     1621 agcagcgcgg gctggtcctt gtgcgcggga tttccggtca cgagacgcag caggcgggcg
     1681 gcctcatcgg ggcggtcgtc atccagcatg catctggcct ggtagagcag ggcgcgggcg
     1741 cggtccggca ccagcacata gaggctgcgc gcgtggtcga gcaggtcggc ggcctcggcg
     1801 ggttttccct cgctggcgga gagcagcgcg gtgagttcca gcagatgagg cttggagcgt
     1861 gacccggcca gcgcggccag ctcggatttc agcgcgcgcg tgtcctcgcc gggatggcgc
     1921 agcgcggcgc cctggtagag tgcgtagtcg cgctcgcggc cctcgcccat ggcgcttccg
     1981 gggcctttgg caaaggggcg gtagagcgga tccccgcaga gcacgttcat ccaggagagc
     2041 accggcgtgg cgttccaggc ggcctcgccg agggtgaagc cttccagcag gcgctggttg
     2101 aggatgtcga aatgcacggt caggctcagg tagggttcga acacattgcc catgaccgcg
     2161 gcggcgccct ggcgcagcag cggaccggcc cagtggcggt ccggggagcg cagcgccgcg
     2221 ccgctgaaac tgtgcaggtg gcaggccacg gcacctgggg tgaagcggaa ggacgggctt
     2281 gccaccgcgc cgctggcgtg cgcggcatac cagccgaaat acagcgccgt gtcaggcagc
     2341 ggccagtggt ccgggatcag cgactcaccg cggtcgagga agacggggat gccctgcccg
     2401 cgcagcagga tggcgctgcg cttcagccag tcctccccct cctggtaagc gccgcttttc
     2461 tgcgccaggt caatgaccgc gcgcccgcgc aggccctcgc gctccgccgc cagggagtcg
     2521 tccaccatgc gcttcaccgt gtcggcgtcc ggtccgtcca gcctggccac gagcaggagt
     2581 cccggcgctg tttggaagag ttgaaagcgc gcgctttgat tgaaatatgg attcggcacc
     2641 gccccggcgg cgggctggcg cggcaggccc agcacgcaca gctcgctgtc caccccggcc
     2701 tcgtcctcct ggctgttctg cgggttctgt ttttcccggc ggatttggaa tggtatgccg
     2761 cgcatgagca ccagcacgcg cgcgctgctt cgcgtcaccg ccatcgccgg cgcgtccctg
     2821 ccggtggcgg gatcggtcaa ggtcacctgg ccaagctccc accagccctt gcgcacaaaa
     2881 gtctcgaaca gcggcctgcg caccctccgc tcgaactcat cgcggctcat cgagtcctcc
     2941 aaagggcact ccagcgccac cagccgctcc tgggggatgc cgcgcatccg cgcataatac
     3001 tgcgccagct cgcgcgactg agcgtagtcg ggattgaagg ccaccactgt ctcagtcgag
     3061 agatcaaagc cgcccgcttc ctgcccgcgc aagccttcca cggccagcag ccaggcggcg
     3121 agccaaaaac ccgcgactcc gcgccggcgg accggtctgc gagggtgtgc tggatcatgc
     3181 gcgtggctca ttgaggtgcg attatcctat ggaagacagc gccgcgcgct tcaaggaaaa
     3241 ctccgcctca cgctggatga gcgcgagtct attcgcgctc aagagcgcct gcgctccccg
     3301 ccgccacagc ccagcgcagc ttggcgggac tcgcgcgcgg gttgtcgcgg atttcctgcg
     3361 gcgaaggacg gatcacctcg tcattgacgg agctgaaaac cccgccgcgc agaccgtcgc
     3421 ggaacacatg cttcacgcgc cggtcctcgc cggagtgaaa ggtgaggatg gccacgcggg
     3481 cacccggtcg cagacaggct ggcagttgcc tgagaaaagt gtcgagtgct tcgaactccc
     3541 cgttcacagc gatgcgcaga gcctggaaga cgcggcgcac cgcagcctcg cgctccctct
     3601 catccagccg ccccggcaaa gctccgcgca ctgcctccgc cagcgcgcgg gtgccggcgt
     3661 gatcccttcc ggcgagagcg cgcgcgagca gcgccgcgtg gcgctcgtcg gcgttctcct
     3721 ccaggatttc cgccagcttt tccacgggga gcttttgcag cagatcgcgc gcggagacac
     3781 cgcgcagggg attcatgcgc atgtccagcg ggccgtcgtg tttgaagctg aatccgcgcg
     3841 cgggattgtc gaactgcatt gaggagcagc ccaggtccgc cagcagcaca tccgcgccat
     3901 cacaccagcc caggtccgcc agcgcgcgcg gcaggccggc gaagttcatg cgcctgacca
     3961 acagcgcgtc ctccccgcag ccgctggcgc gcagcagcgc ttcggcgcgg acaatctcga
     4021 tgggatcctg gtcaagcgcg agcagggcgc ctccgggttg caccgctttg agcagcgctc
     4081 cggcatggcc gccgtggccg agcgtgcagt ccaccgcgcg ctcgccgggc cgcggcgcca
     4141 gcacctccag gacttcccgc aaaaggatcg gcacatgctg gccggcgggc gtttttcccg
     4201 ccgcgcgcac cttcgccacg gtctccgcat ggagttcggg gttcagctcc ttgtatttat
     4261 cctcaaaccg gcgcggattc ttccccggat aacgcgggcg gcggcggtgc ggagctgggt
     4321 tttccggctc gctcacagct cgttgtactt ggcgttcgaa ccgcgcgcct tcgccacggg
     4381 gtagcgcgcc tcgttgcgct cgatcttctc cagcatcgcc tggccgagat tgattccgag
     4441 attgtgcgcc atctcaaaaa gcaggatgcc cacatcggcc atctcgtcct tcaactccgg
     4501 catcctctcc ctcacacgct gctcgatctg gccctgctgc tgccagacaa aatgctggag
     4561 caactccccc gcctccgcag tgatggccac agccatctcc ttcgggtcgt gaaactgcat
     4621 ccattcacgc gcgtcgcgga aggcgcaaat gcgctcggtg atctgctcga tggtcatcct
     4681 ccatcatgca ggctcatccg caccggagca aacactactg agccgtccgt gcgcaccgcg
     4741 tccacccgag cggaggtggt ccgccggaaa ggcggtcaat gagggccgcc agcccgcgtg
     4801 ccgcgcggga acccggagac cgcacggagc gtcgtgcgga tggtgtgccc gtgggagaga
     4861 tggggcaatc aatggcaaaa aatccgcggg gcttgaattg ttggtgggaa ggctttaaca
     4921 cggcggctgc ggttttgagc gccccctttg cgggagtccc ggcccgtgtt tgcaaactga
     4981 tcccccaacc ccctgatgac ccccctctct gatatcgccg ccatcctgcg cggcgctcaa
     5041 cgcatcggca tcgccgcgca tgtgcggccg gatggagatg ccgtcggctc cgtgctcggc
     5101 ctggcgctga gccttcgcgc gctcggcaag acggtgcatg ccctgctgga agacggcgtg
     5161 ccggaaaacc tggccttttt gcccggcgcg gagacgattc tgcagccggc gggtccgctg
     5221 gatctggatg tggcggtggc gctggacacg gcgaactacg agcggctggg cgcgggtgtg
     5281 gtggagatct tcggcaaggc gccggtgctg gtgaacatgg accaccaccc caccaatccg
     5341 ctatacggcc accacaacca catcgcctcc gacgagccgg ctgccggcca gatcgtctat
     5401 gagctgctgg cgcaaaacgg cctgccggtg gatgaagggg tgcgccagaa tctgttcgcg
     5461 gccatcagca cggacacggg ttctttccag ttcgccagca cgacggcgcg cagccatgag
     5521 atcgcggcgg agatgatccg cgcggggctg gacatcgccc gcctctcgca actgctttac
     5581 cacacctttc cgctgcgccg gctggagctg cagcgggcga tgctcaatga gatggccttc
     5641 cgcgcggagg gccggatcgc gagctggcag ctcacgcagg agacgcagca gcgctgcggg
     5701 gtgcgccccg gcgacacgga gggcctgata gacaccctgc gcatgattga cagcgtggtc
     5761 agcgcggtgg tcttcgagga gctaaaggac ggcaaggtgc gcgtgagcgc tcgctcgaag
     5821 gacagccgcc tggatgtgtc cgcggtgtgc gggcagttcg gcggcggcgg gcaccgcatg
     5881 gctgccggcg cgcgcctgcc gggtccgatc cctgcggcgg cggaaacttt tctacaagca
     5941 ctcgagcatg aagtccgacg actctcttaa cggcgtgctc ctggtggaca aggggccgga
     6001 catgacgagc cacgatgtgg tggcggtggc caggcgctgc ctgcgcacca aaaagatcgg
     6061 ccactgcggc acgctggacc cgatggcaac cggaatgctg atcctggtca taggcaccgg
     6121 caccaagctg caggatctgc tgatgagcga ggacaaggaa tacacgggca gtctcaagct
     6181 cggtgtgacc accagcacgc aggaccgcga gggggaggtg ctggaggaga aacccgtgcc
     6241 cgctttcacg gcggacgaga tccaggccgc cttcgacgct tacaagggcg acttttacca
     6301 gacgccgccg atggtcagcg cggtgaagat tgacggcgtg ccgctctaca aactggcgcg
     6361 ccagggcagg gaggtggagc gcaagccgcg cttcgtgcgc gtctatgacg cgcagatcac
     6421 ccgcgcggag ccgccggatg tggacttccg cgtggtgtgc agcaagggct tttatgtgcg
     6481 cacctacgcg cacgacatcg gccagaagct cggctgcggc gcgcatctga ccgcgctgct
     6541 ccgcacccgc agcgggcact tcaagttcca gcccggcaac cacaccaact tcggcgcgct
     6601 caaggagggc aggcgggacg aggtgctggc ggcgatgatg agcctgtacg atgtctccaa
     6661 gctgcggggg gcgtaggctg gtcactcctc aatgtcgccg ctgcgggcga ttttgatgaa
     6721 cacgtcctca agggagtttt cggcgaactt ttggacgatc tgcgcggggg tgccttcggc
     6781 gacgatgcgg cctttgtgca ggaagatgac gcggtcgcag acctcctcga tgtcacgcat
     6841 gttgtggctg gtgtagagga tgctggtgcc gcgctcggct tggactcggc ggacgacttt
     6901 gcggactttg tcggcgatgt cggggtcgag gctggcggtg ggctcgtcga gcatgagcag
     6961 ctcggggtcg ttgaggaagg ccttgcagag gttcacgcgg gtggactcgc cggcggagag
     7021 gcggccggtg acgtggtggg agaggtgcgg tatttccagc atctcgagca gggaggcgat
     7081 tttgtttttg gcgtcgcgca cgccgtagat gcgggcgaag acgagcaggt tttgccagac
     7141 tttgagattg ccgggcaggc tgacgtaggc ggaggaaaag ttcgcgcgct ggaggatttc
     7201 gatgcggtgc ttgtgcaggg gtttgccgaa ggcggacagc tcccccgagg tgggcagggt
     7261 gagtccgagc aggcagttca tggccgtggt cttgcccgcg ccgttggcgc cgagcaggcc
     7321 gagaatctcg cccttgtgca gccggagcga gagccggtcc agcgccttca caccgtcgaa
     7381 ctcccgggtg agttcgcggg cttcgaggat gacttcacgg gggtcggcgt cgggcatgag
     7441 ggcgcgaaga ctcgccgcaa acgcccgctt tgcaaccgcg ccgcccggcc ttctcaactc
     7501 cgcgaaagcg cgcgcctcca gggctgattc gtccgttgac aggccggagg cgcgcggctt
     7561 ggtttgcacc tgtgacgcgg ttccccgcgt ggtcggttca aaactcccct tcgacttatg
     7621 gcgcagcaca gccagcacaa acaggactca tccccagcct gccatggcgg gggcgggcag
     7681 accatcgtcg aattgtgccg aagcacggac agcggcctcg ttttctggtc ccgccagcgc
     7741 tttgagatcg gctccgagct gcaaatccgc atccatcgcg gcgcgctgcc aggttttgcg
     7801 gccaagcaga tcgctgagga gtccgactgg gtgacggtgc gcggcttcgt ggtcgaatgc
     7861 cccgcgatgc gcaggcagga cggctcgcac ggctttcaag tctcactctt gctggacgcg
     7921 gcgctgattg ccccaaaacc ccgccgcccc aggcgcgcgc ggctgctgcc caggatgaac
     7981 acgcgcttcg ccggcctggc gcgtcccggt ttgaactgag cgctgtggcg cggatgcgtg
     8041 gcaaagacgg gcttggcacg ggcgggggtt gccgccagca tgagcgctgt gtctccccgt
     8101 gtcctcatcc tgatgctcat ggcatccggc gcgccgtctt tgcgcgcggc ggacgaggcg
     8161 atcacgactt ggaatccgct gcggctgatg gctgaaaaag ccgggctgcg cgcgcgcatc
     8221 gaggaggacg cgcacaggct cgccgccatg ccggaggcgc gcgtcgtggc gcagtcgcac
     8281 gggcgcgtcg gctttcacgg cagggcggca gcgcccgcct ggatcgtgct cgatctggga
     8341 aaagaggcgg cgccggacga ggtggtgctc ctgcccgcgc gtgtcagtga tcccgcgccg
     8401 gatgatccgg tggagggttt tccgccggaa ttaaaatgcc tgatgtccat gaccgtggag
     8461 gagggttctt ttcaggtcat gaacgagtgg gccgaacccg caggcggtgc tggcgcgagg
     8521 ctggccgcgc tgcgtctgcg caacccgcgc ggcctttccg ggcgattttt gcgcattgag
     8581 atcaccgggg gcaggccgcg cgcgcgaggc atgttctttt ccctcggcga ggtgtgggcg
     8641 ctcaaggacg gagtcaatct cgccctcggc gcgccggtgg aaagcagcgc ctcgattgac
     8701 aatccgccgc gctggacggc ggcgaatctc actgacggct tcatctggtg cggctcaacc
     8761 acgggcacgg agaaggctcg gaaaaatggc ttccacagcc gcattgagtc ctcgccggat
     8821 gccgtgccaa agtgggtgga ggtggtcctg gacgaggcca tgccggtgga tgaaatccgc
     8881 ctcgtcccgg cgcgtccggc tgacttcgcg gatgtgaccg gctttggctt tccgccggag
     8941 ttccaggtgc tggcctgggc ggatgactcg ccggatgaga agcctgtggt ggtctttgac
     9001 acgggcggcg cgggctttgc caatccaggc gatgccacgg tctgtctgcc cgcccatggt
     9061 gtgaagacgc ggcgtgtgcg cgtggtggcc ggcagattgt ggcggcgcac gtcggactac
     9121 atcttcgcgc tggccgagat gcaggtcatg tcggcgggca ggaacgcggc gctgggccgc
     9181 gaggtgaggt tcagcgacat cgtcgagagc gcctcatgga cgccggaggc gctggtggat
     9241 ggattcggca gccagcgcgc gctgctggat gcggcgcgct ggcttgatga gatggatgcc
     9301 cgcgccgcgc tggaagcgga gctgctgccc gcgcgcgcgg ggttggccgt gctggagctt
     9361 gcctcgcagc gccagttgtt gctcgcagtg gagatactcg tcatcgcgat gctggtggtg
     9421 ctggcggcgg cggccatcct gctcgtccac cagcgccggg cgcgcgccga actgcgcgcc
     9481 cgcatcgcgc gcgacctgca tgacgagatc ggcagccagc tcagccatct ggctctgctg
     9541 gccgagaccg gcggcgcgga tgcggacgcg gctctgcgcg ccatcaagtc cggcgcgcga
     9601 gagacgcagc aggc
//
LOCUS       DAOMYJ010000198         9571 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Prosthecobacter sp. isolate AMR_MDS_5629
            NODE_2376_length_9571_cov_20.218159, whole genome shotgun sequence.
ACCESSION   DAOMYJ010000198 DAOMYJ010000000
VERSION     DAOMYJ010000198.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867850
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prosthecobacter sp. (wastewater metagenome)
  ORGANISM  Prosthecobacter sp.
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Verrucomicrobiaceae; Prosthecobacter.
REFERENCE   1  (bases 1 to 9571)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9571)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.30
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:34:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,742
            CDSs (total)                      :: 4,684
            Genes (coding)                    :: 4,676
            CDSs (with protein)               :: 4,676
            Genes (RNA)                       :: 58
            tRNAs                             :: 55
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9571
                     /organism="Prosthecobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2376_length_9571_cov_20.218159"
                     /isolate="AMR_MDS_5629"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1965333"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1580
                     /gene="katG"
                     /locus_tag="PLP58_17170"
     CDS             <1..1580
                     /gene="katG"
                     /locus_tag="PLP58_17170"
                     /EC_number="1.11.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018289944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004096 - catalase activity [Evidence
                     IEA];
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="catalase/peroxidase HPI"
                     /protein_id="HRJ10313.1"
                     /translation="ENPLAAVQMGLIYVNPEGPNGNPDPLAAAKDIRETFGRMAMNDE
                     ETVALIAGGHTFGKAHGAGPADNVGPDPEAAPLEEQGLGWKNKFGKGNAGDTITSGLE
                     GAWTSAPARWSHMYLSNLYAYEWVLTKSPAGAQQWTPKDGAGQGTVPDAHDKNKSHAP
                     IMFTTDIALKMDPEYAKVTKRFLENPKEFEVAFAKAWFKLTHRDMGPLARYHGPEVPK
                     ETFVWQDPVPAVDHPLVNDQDIAQLKAKLLDSGLTTSELAATAWASAASFRGSDKRGG
                     ANGARIRLAPMKDWEVNNPAQLAKVLAKLESIQAEFNAAQSDGKKISMADLIVLGGSA
                     AVEAAAKKAGHDVTVPFTPGRTDATQEMTDVESVAYLEPKQDGFRNYLGHALDRPGPE
                     TLVDRAQLLNLSAPEMTALVGGLRALGTNTGHPDLGVLTKNRGALTNDFFVNLLDLDT
                     EWKVSPRCEHFYEGTDRKTGEMKWMATSVDLVFGSNSQLRAIAEVYASSGNEKKFVND
                     FVAAWTKVMNLDRFDLK"
     gene            complement(1653..3029)
                     /locus_tag="PLP58_17175"
     CDS             complement(1653..3029)
                     /locus_tag="PLP58_17175"
                     /inference="COORDINATES: protein motif:HMM:NF019113.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HRJ10314.1"
                     /translation="MKAILKAPNADLAVSAVPNVMPDSAGGEVPPMVFPPAGNAYNQS
                     DNDAAHYLWRWIGMHAPDAVILVAAKGDASAASLAKALGETAPCGVGTIPCSVVTPTG
                     VQQALASAAANAQPSPARWEMRRRIARSATGVAAELAEVHGHKLDAVAYIPALAIVGR
                     MRLGELTGGDAALRDAEKICAPYKTGGKESMPSNGNGGTISGHLVFAELARRSGDPDW
                     TALVKKAADYGFDAQGQPKESMPFHSEMSDSVFMGCAILADAGALTGDKRYFEQCLRH
                     LRFMEKRCLRADGIYRHSPLDESAWGRGNGFPALGLSLSLSSLPPESTEHAAFLRSYR
                     AHMAALRMHQDPTGAWHQVIDHPESYREFTSTCMITFAIVRGLRRGWLETAEWQPVVD
                     KAWTAIKSRIGNGGHLVDVCTGTGKMNSLRDYLDRTAILGRDDRGGAMALMVSTEVAF
                     WEKEKIVR"
     gene            complement(3482..5041)
                     /gene="malQ"
                     /locus_tag="PLP58_17180"
     CDS             complement(3482..5041)
                     /gene="malQ"
                     /locus_tag="PLP58_17180"
                     /EC_number="2.4.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009959266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004134 - 4-alpha-glucanotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0005980 - glycogen catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-alpha-glucanotransferase"
                     /protein_id="HRJ10315.1"
                     /translation="MFPIPRSSGILLHPTSLPGRHGIGEIGPEALRWLEELARMGQRL
                     WQMLPLGPTGYGASPYQSLSSFAGNPLLISFDSLIMDGVLTPGEVAGLPHFPEEQVLF
                     TEVEDARNDLLKTAAGRFLKQCARSPLVRRAFEAFCESESSWLDDYAIFIALKVRHGG
                     LPWHEWPEEVRRRSQTALEQAGAELRRETDEVRALQFFFHRQWRKVRARALELGISLI
                     GDIPIFTAHDSADVWARPELFELDENGAPLVVAGVPPDYFSATGQRWGNPLYRWPSHQ
                     AEDFSWWQARLRKTLEMVDIVRIDHFRGFAAYWEIPAGEPTAVNGRWVEAPGDALFEA
                     LKRGLGAELPVIAEDLGIITPDVTALRERHGFPGMKVMQFAFGADTLAQDYIPENYPD
                     DCVAYTGTHDNDTVLGLFRSGEGGDTTRTAAQIEAERRTILGYTGTDGSELHWDFIRA
                     VLGSRARLAITPLQDLLGLGSEARMNTPGKMGASFWSWRFTWDQLTPKIVEKMRGLTQ
                     TSGRARDSDHA"
     gene            complement(5162..5941)
                     /gene="panB"
                     /locus_tag="PLP58_17185"
     CDS             complement(5162..5941)
                     /gene="panB"
                     /locus_tag="PLP58_17185"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: protein motif:HMM:TIGR00222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003864 - 3-methyl-2-oxobutanoate
                     hydroxymethyltransferase activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HRJ10316.1"
                     /translation="MLTSLQDLPHRKKDGPPLAVLTAYDYPLARLLDEAGVDLILVGD
                     SLGMVVLGLPDTTGVTMEMMLHHLAAVRRGVVRAPVIGDLPYHSYRTPGVAAQNARRL
                     VEAGADAVKLEGGADFVPQARAIIQAGIPFVGHIGMLPQSVREEGGYKKKGRTPEDAA
                     RLLADARALDDAGACAIVLESMMHDAAAVITRQVSASTIGIGAGPATDGQVLVTHDLI
                     GAFPWFRPPFAKARADIAAECSRAAREFVRSCWEPSGEKHG"
     gene            complement(6054..6743)
                     /locus_tag="PLP58_17190"
     CDS             complement(6054..6743)
                     /locus_tag="PLP58_17190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ10317.1"
                     /translation="MSPTLPPPSLGSSYAGPTMMEPVPPPPSPYGFQQPTPTNTWGSI
                     QESAEGSMLNYQYLEVGYRYLDPKGGDYNGSHGLGGTLVLKLPAMFFLKGSVNWTSGS
                     GNKTVRGVKDADYSLSTITIGGGLFMAIKPNFHFVGEAGFVYANFDGANRNYSFTEAG
                     IYIRPSLRYQMVDWLELQTGVTVTSTSDFDNRIWDLAGYFRVMPQFDLNIGFDIGNES
                     RVLRAGGRLRW"
     gene            complement(7077..8807)
                     /gene="recJ"
                     /locus_tag="PLP58_17195"
     CDS             complement(7077..8807)
                     /gene="recJ"
                     /locus_tag="PLP58_17195"
                     /inference="COORDINATES: protein motif:HMM:TIGR00644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008297 - single-stranded DNA
                     exodeoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HRJ10318.1"
                     /translation="MALASEKPLSNTPAPRWLLKPVPEGAAELALDTGMPPLIATLLM
                     QRGVTTPDMIRDFLVPKLASLSDPCRLPEMSLAVERILRAVDEKQRVALYGDYDVDGV
                     TSMALMHLTLKAYGLDTHLFLPSRMEEGYGLSLDGFARLFEEFGKPDLLIALDCGTTS
                     INELAWLREQGVDCVIVDHHELSPFGRPPCAALVNPKLGQDYHYFCTAGLVFKVAHAL
                     LKTRRVESFDLKEALDLVALGTVADLVPLVDENRLLVRRGLEALAETQRIGLRALKQS
                     AGVDGFIQTHHVGFRLGPRLNAAGRLDTAATALQLLLAEDPEIANEYAALLESHNRDR
                     QAVELEVFNEAERMVLEMGSLDEVPAIVLGSRNWHPGVVGIVASRISRIVHRPTILFS
                     FDENGMGKGSGRSIPGFSLVEAIDYCREHIERGGGHAMAAGFSVLEDKLDIFRKRFCE
                     AARNVLGPDAPAPVLEFDAEVRLRDLSLDFLEYYRLLEPFGQRNPEPVFLCRGVTPRL
                     PGRWMKEKHLRVMLTQDGASMEARWFNAPAGNLPKPPWDLAMRLQRNTWRGEEQWQLT
                     LEAVKSSGGE"
     gene            8874..9380
                     /locus_tag="PLP58_17200"
     CDS             8874..9380
                     /locus_tag="PLP58_17200"
                     /inference="COORDINATES: protein motif:HMM:NF023580.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3592 domain-containing protein"
                     /protein_id="HRJ10319.1"
                     /translation="MSSSAESTLAGRVWLACMGLFIAAAGLLFTCVLWTSWQRAEETR
                     RWTPLPCRIVSARVVSEKPTPNSNPAHKAEIRYEYTFQGKPRTGTRIKRVDAASQHEE
                     RARQKLEAWPVGTEAACFVNPAAPDEAVLAHDTRAALYSIWFPLLFVFGGFGMAWNAL
                     RPRPANTL"
     gene            complement(9383..>9571)
                     /locus_tag="PLP58_17205"
     CDS             complement(9383..>9571)
                     /locus_tag="PLP58_17205"
                     /inference="COORDINATES: protein motif:HMM:NF015129.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ10320.1"
                     /translation="GAEIFDVFTDSAGMKIAADRKSTAWKLTYRASDKTLETAEVDAV
                     HSRIVAALEKSLPAAVRR"
BASE COUNT         1836 a         3043 c         3049 g         1643 t
ORIGIN      
        1 tcgagaaccc cctcgccgcc gtgcagatgg gcctcatcta cgtcaatccg gaaggtccaa
       61 acggcaaccc cgacccgctg gccgccgcca aggacatccg cgagaccttt ggccgcatgg
      121 ccatgaacga cgaggaaacc gtggcgctca tcgccggcgg gcacaccttt ggcaaggccc
      181 acggcgcagg ccctgcggac aacgtcggac ctgatcccga agccgctcca ctggaggagc
      241 agggcctcgg ctggaaaaac aaattcggca agggcaacgc cggtgacacc atcaccagcg
      301 gcctcgaagg cgcctggacc agcgcccctg cccgctggtc ccacatgtat ctttccaacc
      361 tctacgccta tgagtgggtg ctgacgaaga gccccgcagg cgcgcagcag tggacgccga
      421 aagacggcgc tggacagggc accgtgccgg acgcgcatga caagaacaag agccacgcgc
      481 ccatcatgtt cacgacggac atcgcgctga agatggaccc ggaatacgcc aaggtcacca
      541 agcgcttcct ggagaacccg aaagagttcg aggtcgcctt cgccaaagcc tggttcaagc
      601 tcactcaccg cgacatggga ccgctcgcgc gctaccacgg cccggaagtg ccaaaggaaa
      661 ccttcgtctg gcaggacccc gtgcccgccg tggaccatcc gctggtcaat gaccaggaca
      721 tcgcccagct caaagccaag ctgctcgact caggccttac cacctccgaa ctcgccgcca
      781 ccgcctgggc ctccgccgcc tccttccgcg gcagtgacaa gcgcggtgga gccaatggcg
      841 cgcgtatccg tctcgccccg atgaaggatt gggaagtcaa caaccccgcg cagctcgcca
      901 aagtgctggc gaagctggag tccatccagg cggagttcaa cgccgcccag tccgacggca
      961 aaaaaatctc catggccgac ctcatcgtgc tcggaggcag cgccgccgtc gaagccgccg
     1021 ccaagaaagc gggccatgac gtgaccgtgc ccttcacccc cggccgcacc gacgccaccc
     1081 aggagatgac cgatgtcgag tccgtggcct acctcgagcc aaagcaggac ggcttccgca
     1141 actacctcgg ccacgccctg gaccgccccg gcccggagac actggtggac cgcgcccagt
     1201 tgctgaatct ctccgccccg gagatgaccg cgctcgtcgg cgggttgcgc gcgctcggca
     1261 ccaacaccgg ccaccccgac ctcggagtgc tcaccaagaa ccgcggtgcc ctcaccaacg
     1321 acttctttgt gaacctgctc gacttggaca ctgagtggaa agtctcccca cgctgcgagc
     1381 acttctacga aggcaccgac cgcaagaccg gcgagatgaa atggatggcc acctccgtgg
     1441 acctcgtctt tggctcgaac tcccaactcc gcgccatcgc cgaagtctat gccagctccg
     1501 gcaacgagaa aaagttcgtg aacgacttcg tcgccgcctg gaccaaggtc atgaatctgg
     1561 accgtttcga cctgaagtga tctgaaaccc aaacaatccc cgccaacggc ccatcccggt
     1621 gaaaaccggg atgggctttt tgcattcgca ttttaacgca cgatcttttc cttctcccaa
     1681 aaggcgacct cggtggacac catgagtgcc atcgctccgc cgcgatcatc gcggccaagg
     1741 atggcagtgc ggtcgaggta gtcgcgaagg gagttcatct tgcccgtgcc ggtgcagaca
     1801 tccacgaggt gaccgccatt gccgatgcgg ctcttgatcg ccgtccatgc cttgtccacc
     1861 accggctgcc attccgccgt ctccagccag ccgcgccgaa ggccgcgcac gatggcgaag
     1921 gtgatcatgc aggtgctggt gaactcccgg tagctctccg gatgatcaat cacctggtgc
     1981 cacgctcccg tcggatcctg gtgcattctc aaggcggcca tgtgagcgcg atagctccgc
     2041 aggaacgcgg cgtgctccgt ggactctggc ggcagcgaac tgagggaaag cgagaggccc
     2101 agcgccggga agccgttgcc gcggccccag gcggattcat cgagtggcga atgacggtaa
     2161 atgccatcgg cgcgcaggca gcgcttttcc atgaagcgca ggtggcgaag gcactgctcg
     2221 aagtaccttt tgtcaccggt caaagccccg gcatcggcca ggatcgcgca gcccatgaag
     2281 acggagtcgc tcatctcgct gtgaaagggc attgactcct tcggctgtcc ctgagcgtcg
     2341 aagccgtaat cggcggcttt tttcacaagg gcggtccagt cgggatcgcc cgagcggcgg
     2401 gccagttcgg caaaaaccag gtgccctgaa atcgtgccgc cgttgccgtt ggagggcatg
     2461 gactcttttc caccagtctt gtaaggcgcg cagatttttt ccgcgtcacg cagggccgcg
     2521 tcgccgccgg tcagctcacc cagccgcatg cggccaacaa tcgccagagc gggaatgtag
     2581 gccaccgcgt cgagcttgtg tccatggact tcagccagct ccgccgccac tccagtggcg
     2641 ctgcgcgcta tgcggcgccg catttcccac cgcgcaggtg agggttgagc attggccgcc
     2701 gctgatgcca gtgcttgttg aaccccggta ggagtaacca cgctgcacgg gatcgttccc
     2761 acaccgcagg gagccgtctc gcccaaagcc ttcgccaatg aagccgccga ggcatccccc
     2821 ttcgcggcca caaggatcac cgcatccggc gcatgcatcc cgatccagcg ccagagatag
     2881 tgggccgcgt cgttatcgga ctggttgtag gcattgcccg caggtgggaa aaccattggc
     2941 ggcacctcgc cgcctgccga atcaggcatc acattgggaa ccgctgaaac cgccagatcc
     3001 gcattcggcg ccttgagaat ggctttcact gcctccactg attccatctt cccgtccatc
     3061 cctccgacca gcagcacgcg atgcctgtca gagtcaaagt tcaagccatc agccgggatg
     3121 aggcatgcga tagtcgtgtc cgcgcgcgtc actccaacag cagacaaaat cattccatca
     3181 ataccggcgg attccgccgc ctgcaggctg cgcacggagc ctgcggatgc taatgtcatc
     3241 aagacggagg cgaacaaaga cgacgtagat ttgatccagg atggaaagtg cattcgtctc
     3301 acctatcgtc cttgcgaaga cgagtcaagc cagggcgcgg accacgcgcg cctcatgtcc
     3361 acccctgtca gcctttggtg ccaatgcgga attgatcggc tttgctgcca tgcgctggat
     3421 cttatgcccg cgagttgcgc gtagatgacc gagaagcggc gccgaagctg agggatgtag
     3481 atcaagcatg gtcggaatcg cgggcgcgtc cggaggtttg tgtgaggccg cgcatttttt
     3541 ccacgatctt cggggtgagc tggtcccagg tgaagcgcca gctccagaag ctggcgccca
     3601 ttttccccgg tgtgttcatg cgggcctcgc tgccgagtcc gagcaggtcc tgcaaaggtg
     3661 tgatggcgag gcgcgcccgc gagccgagca cggcgcggat gaagtcccag tggagctcgg
     3721 agccgtcggt gccggtgtaa cccaggatgg tgcggcgctc ggcctcgatc tgcgcggcgg
     3781 tgcgggtggt gtccccgccc tcgccgctgc ggaagaggcc gaggacggtg tcgttgtcat
     3841 gcgtgccggt gtaggcgacg cagtcgtcgg ggtagttttc ggggatgtaa tcctgcgcca
     3901 gggtgtcggc accgaaggcg aactgcatga ctttcattcc tggaaagccg tgccgctcgc
     3961 gcagggcggt gacatccggc gtgatgatgc caaggtcctc ggcgatgacg ggcaactcgg
     4021 cgccaaggcc gcgcttgagc gcctcgaaga gcgcgtctcc cggcgcctcg acccagcggc
     4081 cgtttacggc ggtgggctcg ccggcgggaa tctcccagta ggcggcaaag ccgcggaagt
     4141 ggtctatccg cacgatgtcc accatttcca gggttttgcg caggcgggcc tgccaccaag
     4201 agaagtcttc ggcctgatgg gagggccagc ggtagagcgg gttcccccag cgctggccgg
     4261 tggcgctgaa gtagtcgggc ggcacgcccg cgacgacgag gggcgcgccg ttttcatcca
     4321 gctcgaagag ctccggccgc gcccacacgt ccgcgctgtc gtgcgcggta aagatgggga
     4381 tgtcgccgat gagggagatg cccaactcca gcgcgcgggc gcgcactttg cgccactgcc
     4441 ggtggaagaa aaactgcagc gccctcacct cgtcggtctc ccggcgcagt tccgcgccgg
     4501 cctgctccag ggctgtctga ctccggcggc ggacttcctc cggccactca tgccacggca
     4561 gcccgccatg ccggaccttc aaggcgatga agatggcgta atcgtcgagc caggaggact
     4621 cgctctcgca gaaggcttcg aaggcgcgcc tgaccagggg gctgcgcgcg cattgtttga
     4681 ggaagcggcc agccgctgtc ttgagcaggt cgttgcgcgc gtcctccacc tcggtgaaaa
     4741 gcacctgctc ctccgggaag tgcggcagcc cggcaacctc gcccggagtc agcacgccgt
     4801 ccatgatgag ggagtcgaag gaaatgagca ggggattgcc ggcaaaactg gacaggctct
     4861 ggtagggcga ggcgccgtag cccgtggggc cgagcggcag catctgccag agccgctgcc
     4921 ccatgcgggc cagttcctcg agccagcgca gagcctccgg cccgatctcg ccgatgccgt
     4981 gcctgccggg cagggaggtg ggatgcagga ggatgccgct ggagcgtggg atgggaaaca
     5041 tgggggctga ggaataagat ggaaggaggc gagtgaaagg aaaaaagcgg ccggcactgc
     5101 ctgagcacca accatgcccg gagtgttcag aatgagacgc tggcgcgctt catcccgccg
     5161 gtcagccgtg tttttccccc gacggttccc agcacgagcg cacaaactcg cgcgcggcgc
     5221 gactgcactc ggctgcgatg tcggctcggg ctttggcaaa gggcgggcgg aaccagggga
     5281 aggcgccgat gaggtcgtgg gtgacgagca cctggccgtc tgtcgccggc cccgcgccga
     5341 tgcctatggt gctggcgctc acctggcggg tgatcaccgc ggcggcatcg tgcatcatgc
     5401 tttcaaggac gatggcgcag gctccggcat cgtcgagcgc gcgcgcgtcg gccagaaggc
     5461 gggcggcatc ctcaggggtg cggccctttt ttttgtagcc gccctcctcg cgcacgctct
     5521 gcggcagcat gccgatgtgg ccgacaaagg ggatgccggc ctgaatgatg gcccgcgcct
     5581 ggggaacgaa gtcagcgcca ccttcgagct tcaccgcgtc cgctccagcc tcgacgaggc
     5641 ggcgggcgtt ctgtgcggcc acgccgggcg tgcggtagga gtggtaaggc aggtctccga
     5701 tcactggagc gcggacgacg ccgcggcgca cggccgcgag atggtggagc atcatttcca
     5761 tggtaactcc ggtggtgtcg ggcagcccaa gcacgaccat gccaagagag tccccgacca
     5821 ggatgaggtc cacgccggcc tcgtcgagca ggcgcgccag gggataatca taggcggtga
     5881 ggacggcgag cggcgggccg tctttttttc ggtgtggaag atcctggagg gaagtcagca
     5941 tgatggaggg aacgggcgca gtctgccgtc atcgcagcgt gatgacagca aaaaagcgcc
     6001 ctgtcaccag gacgcctttt tgtgtcttga gcgcgggaga aaaatgacgc ggctcaccag
     6061 cggagacgac cgccggcgcg cagcacgcgg ctttcattgc cgatgtcgaa gccgatgttc
     6121 aagtcaaact gcggcatgac gcggaaataa ccggcgagat cccagatgcg gttgtcgaaa
     6181 tcactggtgc tggtgacggt gacgccggtc tgaagctcaa gccagtccac catctggtag
     6241 cgcaggctcg ggcggatgta gatgcccgcc tcggtgaagc tgtaattccg gttggcaccg
     6301 tcaaagttcg catacacaaa gcccgcctcg ccgacaaagt gaaagttcgg cttgatggcc
     6361 atgaacaagc cgccgccgat ggtgatggtg gacaggctgt aatccgcgtc cttgacgccc
     6421 ctgacggtct tgttgccgct gccgctggtc cagttcacgc tgcctttgag gaaaaacatg
     6481 gccgggagtt tcagcaccag cgttccaccc agaccgtggc ttccattgta gtcgccgcct
     6541 ttcggatcga gatagcggta gccgacctca aggtattgat agttcagcat gctgccctcg
     6601 gcgctttcct ggatgctgcc ccaggtgttt gtgggcgtgg gctgctgaaa gccatacggt
     6661 gaaggcggcg gaggcactgg ctccatcatc gtgggaccgg catatgagga acccagcgaa
     6721 ggcggcggga gtgtggggga catgccggga taggcatttg aagccggggc accataggcg
     6781 gcagggggag gcggggcact gtaggttgaa ggcggcgctc cgtagttggg cggaggcgcg
     6841 ctgtaattca ccggtggata ggtgggaggc ggagtcaggc ctgcgccagc cggaggatag
     6901 ccataattct gcgccaaaag cactggagtg gtcagtgcaa aggagacgag agagcaaagc
     6961 acgctggatt tcatgaatgt gctgattatt tggatttgtt aaataatctt gcaaagtgga
     7021 aaaacagcaa gttttttctg aaattgttgt gatttcagac cttcgagtga ttcaaatcac
     7081 tcgccaccgc tggatttcac ggcttccaac gtcaactgcc actgctcctc cccacgccag
     7141 gtgtttcgct ggagacgcat ggccaggtcc cagggcggtt tgggcaggtt gcccgcaggc
     7201 gcgttaaacc agcgcgcctc catggaggct ccatcctggg tcagcatgac tcgaagatgc
     7261 ttttccttca tccagcggcc gggcaggcgc ggggtcactc cccggcatag gaagaccggc
     7321 tccgggttgc gctggccgaa gggttcgagc aggcggtagt attcgaggaa atccaggctc
     7381 agatcacgca gacgcacttc ggcgtcgaac tctagaaccg gagcgggcgc gtcgggtcca
     7441 aggacatttc tggccgcctc gcagaatcgc tttcggaaga tgtcgagctt gtcttccagc
     7501 accgaaaaac ccgccgccat cgcatgacct ccgccgcgct caatgtgctc gcggcagtag
     7561 tctatggcct cgacgagcga aaagcccggg atgctgcgcc cgctgccctt gcccatgccg
     7621 ttttcatcaa aggaaaatag aatggtcggg cggtggacga tgcgcgagat gcgggacgcg
     7681 acaatgccga cgactccagg gtgccagttg cgcgagccta gaacgatggc gggcacctcg
     7741 tcgaggctgc ccatttccag caccatgcgc tccgcctcat tgaagacctc cagttccacc
     7801 gcctggcggt cgcgattgtg gctctccagc agggcggcgt attcgttggc gatctcggga
     7861 tcctcggcaa gaaggagctg caaggcggtg gcggcggtat cgagacgacc cgcggcattc
     7921 agacgcggac cgagacggaa accgacatga tgggtctgaa taaaaccatc cactccagcg
     7981 gactgcttca aggcgcgcag accgatgcgc tgcgtctccg caagcgcctc caggccgcga
     8041 cggacgagga gccggttttc atccaccaac ggcacgagat ccgccacggt gccgagagcg
     8101 acaagatcga gcgcttcttt caagtcgaaa ctctccacgc ggcgggtctt gagcaaggca
     8161 tgcgcaacct taaagaccaa gcctgccgtg caaaagtagt gatagtcctg gcccagcttc
     8221 ggattgacca aagccgcaca tggaggacgg ccaaaaggag acagctcatg gtggtccacg
     8281 atcacgcagt ccacgccctg ctcgcgcagc caggcgagct cgttgatgga ggtggtgccg
     8341 cagtcgaggg cgatgaggag gtccggcttg ccaaactcct cgaacaggcg cgcgaagccg
     8401 tcgaggctga gtccatagcc ctcctccata cgcgagggca ggaagaggtg cgtgtcgaga
     8461 ccgtatgcct tcaaggtcag gtgcatcagc gccatggagg tgacgccgtc cacatcgtag
     8521 tcgccgtaga gggccacacg ctgcttttca tccaccgcgc gaaggatgcg ctcgacggcg
     8581 aggctcatct cgggcaggcg gcagggatcg gacaggctgg cgagctttgg caccaggaaa
     8641 tcgcggatca tgtccggcgt ggtcacaccg cgctgcatga gcagggtggc gatcagcggc
     8701 ggcatgcccg tgtccaatgc cagctcggca gccccctccg gcacgggctt gagcagccag
     8761 cgcggagcgg gggtgtttga caggggcttt tcagaggcga gcgccatgga tcaagtgcaa
     8821 cctccttgta tgcagcaggc tcgtaggcta gtcaaagcat ccctccccct ttcatgtcct
     8881 cctctgctga atccacactc gcaggccgcg tctggctggc ctgcatgggc ctgttcatcg
     8941 ccgcagccgg cctgctcttt acctgtgtgc tctggacctc ctggcagcgc gccgaggaaa
     9001 cgcgccgctg gactcccctg ccctgccgca tcgtctccgc gcgcgtcgtt tcagaaaagc
     9061 ccacgccaaa ctccaacccc gcgcacaaag ccgaaattcg ctacgaatac accttccaag
     9121 gcaagccgcg caccggcacc cgcatcaagc gcgtggacgc cgccagccag catgaggaaa
     9181 gggcgcgcca aaagctcgaa gcctggcccg tcggcaccga ggccgcctgc tttgtgaatc
     9241 cggcggcacc cgatgaagcc gtgctcgcac acgacacccg cgccgctctc tattccatct
     9301 ggttcccttt gctcttcgtt ttcggcggct tcggcatggc ctggaacgcc cttcgtccca
     9361 gacccgccaa caccctgtaa aatcaccgcc tcaccgcagc gggcaggctt ttctccagcg
     9421 ctgcgacgat gcgtgaatgc acagcgtcca cttcagcagt ctccaatgtc ttgtccgaag
     9481 cgcggtaagt aagcttccag gcggtggatt tgcggtcggc ggcgattttc atcccggcgc
     9541 tgtcggtgaa gacatcgaag atctccgcgc c
//
LOCUS       DAOMYJ010000199         9464 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Prosthecobacter sp. isolate AMR_MDS_5629
            NODE_2414_length_9464_cov_17.403762, whole genome shotgun sequence.
ACCESSION   DAOMYJ010000199 DAOMYJ010000000
VERSION     DAOMYJ010000199.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867850
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prosthecobacter sp. (wastewater metagenome)
  ORGANISM  Prosthecobacter sp.
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Verrucomicrobiaceae; Prosthecobacter.
REFERENCE   1  (bases 1 to 9464)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9464)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.30
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:34:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,742
            CDSs (total)                      :: 4,684
            Genes (coding)                    :: 4,676
            CDSs (with protein)               :: 4,676
            Genes (RNA)                       :: 58
            tRNAs                             :: 55
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9464
                     /organism="Prosthecobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2414_length_9464_cov_17.403762"
                     /isolate="AMR_MDS_5629"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1965333"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(95..1003)
                     /locus_tag="PLP58_17210"
     CDS             complement(95..1003)
                     /locus_tag="PLP58_17210"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: protein motif:HMM:NF014177.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009007 - site-specific
                     DNA-methyltransferase (adenine-specific) activity
                     [Evidence IEA];
                     GO_process: GO:0006306 - DNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Dam family site-specific
                     DNA-(adenine-N6)-methyltransferase"
                     /protein_id="HRJ10321.1"
                     /translation="MPPLKSQGIKTKLVPWIRDLAPVFQGRWIEPFLGTGVVALNCGG
                     SQALLGDINPHIIRLYQAIQSGEITAGSVRKHLEKEGAILSASDEDGYAHFRYLRDRF
                     NSEHNPLDLIFLSRAGFNGMMRFNRKGAWNIPFCKKPGRFAPAYVTKIVNQVADAAKI
                     IQPGWQFITADFEKIIKMAGPDDMIYCDPPYLGRHVDYFSNWTEEDERRLSGLLTATP
                     ARFILSTWHHNDYRKNEAIPAYWSGFNVITRDHFYHTGGKLENRRSVVEALVYNFEAS
                     MPAHTQKREIRLKQYDLFADLLTEVA"
     gene            complement(1012..1929)
                     /locus_tag="PLP58_17215"
     CDS             complement(1012..1929)
                     /locus_tag="PLP58_17215"
                     /inference="COORDINATES: protein motif:HMM:NF020794.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II restriction endonuclease"
                     /protein_id="HRJ10322.1"
                     /translation="MKKHAALKKAFMAALTQFVTELRDHVADEDGQWSVKGFIDIYRN
                     VFTVSSDTKIVSKILEIHIFPKLLAFAGQQGYSIVLAEKQNWYPDLSFVSKADPGLKF
                     AVDLKTSYRDPEFTGHVNGFTLGSHGAYFKDRASTKNIQFPYASYAAHICLGVIYTRV
                     GADELDETRTFSVKELGHVTRKQKAEGSAAIVEDLRSITSVVRDFEFFVCEKWQLASD
                     KQGSGNTANIGAITWIEDIRRGHGVFSRLGEKWFDEYWMNHGQATMLREGKSVPIRTL
                     ADFMDFKGADKKLIVPVRTKTNPRKSKGL"
     gene            2257..>3746
                     /locus_tag="PLP58_17220"
     CDS             2257..>3746
                     /locus_tag="PLP58_17220"
                     /inference="COORDINATES: protein motif:HMM:NF041539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choice-of-anchor R domain-containing protein"
                     /protein_id="HRJ10323.1"
                     /translation="MKTRTHLLALALLLPAATALAEDVVSNLDNASTAQIALSSVGAS
                     AAQAFTTGPEARKLDRIIVRLRGFQAGPHTARVKLWTGSGTLPVTLVEDLGTVPNIPS
                     GNDGEMTVLSTTNPDLAPNTRHWVSVSVSAGDFDWRNTSDPASTGTGAFVQLRATSPS
                     GNAGTWTEPGFATSANFQLMEVRPIVPQTFTVTTTADAGGGSLRQAVADANANPGADI
                     IVFDSDLDGQTITLTTGQLLLTDDVTIDASALPGGISISGNADDNGTLDPGESRIFEV
                     ATGKVVVLRALRLVKGQASTTAGDNSGRGGAIQTRGNLTLDRCLVADSRARFGGGIDI
                     TGGTLTITQSTLSGNSASQGGGIVVFGGFVELTQCTLSGNSATRGGGIYCIGGSLTIT
                     QSTLSGNSASEGGGIFSNPGSLTITRSIVAGNLTGGDIDGSFTDNGNNLTSGDPLLAP
                     LGDYGGPTPTMPPLPGSPAIDAGGSTTETTDQRGFPRVAGAAVDIGA"
     assembly_gap    3747..3846
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <3847..6971
                     /locus_tag="PLP58_17225"
     CDS             <3847..6971
                     /locus_tag="PLP58_17225"
                     /inference="COORDINATES: protein motif:HMM:NF015140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="choice-of-anchor Q domain-containing protein"
                     /protein_id="HRJ10324.1"
                     /translation="TDQRGFPRVAGAAVDIGAVEAQAIPGFDPAVVTSLTDTFDGLPN
                     GVSLREAVAFSPPGATLTFAPGLDGQTITLGGSQILLDKSVTIDASALPGGISISGNA
                     NANGTLDDGESRIFEVATGKVVVLRALRLIKGQASTTAGNNSALGGAILTEGSLNLDR
                     CFVSECRALFGGGIGCVGGSLTITQSTLSGNSASDSGGGIVVLGGSVEITQSTLSGNS
                     AFSGGGIHLSSGSVEITQSTLSGNSASDSGGGIRSSGGSFLTLTRSIVAGNLTGGDID
                     GSFTDNGNNLLSGDPLLAALGDYGGPTPTMPPLPGSPAIDAGGAAASAFPTDQRGFPR
                     VAGAAVDIGAVEFDLDIDSGLPGTLAFGSALTTVLEEAGAGMVPVVRTGGITGSVSVT
                     VNSAPGTATAADFDAVSGLVLTFAQGERQKLVPVTVLADTLKEPTELFTLTLSAPTGG
                     AALGTPNPGIVRIIDSALDKAKPALSLLTPKNNEIILEAAGNVVALTGSAKDNIGIER
                     VEISLDGGDFQPAALTHAADFKSCTFALPLTVTPGLRTVLVRAVDTRGLLSAPVKRAF
                     TFRVVRQLAVNVSGPPGSGTVTKGFEPNSQRFVGIPHTITAAPKPGFVFTGWTANDFT
                     GTGVTTAARELPKLAFLMREGLVLTANFLANPFTPQITGIHSGLALPDAGTTPGAGTV
                     GLFTATVTGTGAFTGSLMLDGAKLPVSGLFTHQGQSRFGKTRSPALVLLRKGKPALTI
                     TLALDMSGATGRITGTVTQEGGATSDITADRAHYSSKSPADPALAGAKGQRYNLILPA
                     KAQTPARALNLYPQGTGIGGMTVKPDGKTSFTLLLADGTKLTAAAALSKNDDCPLFAQ
                     LYGKLGLLAAQVTLAPAQPATDATGPDSVWFRPAQPKATSYRLGWPQGVSVDLFGSQF
                     HIPPAAPPASVFPGLGAVDLINGNAALTFTGGLLVISPSTLGLNIDPANKVTNAPAAD
                     KTFTAALTKTTGEWKGVFTHSDGKKPAWQAATFQKAGAHQGGHGFFLSLPAKPADGLA
                     ESGAVRIQAR"
     gene            complement(7129..7869)
                     /locus_tag="PLP58_17230"
     CDS             complement(7129..7869)
                     /locus_tag="PLP58_17230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ10325.1"
                     /translation="MFEYFHFSLPRAVTEQLVERLAALTATPLNESALRELQAFQSRS
                     KTCQGVYVIYHEGAAAYAGKAENTAERLFQHLAKLSGRSGMDMSHVGFKALLLDENWS
                     TSANEDLLIGHFKKLGECRWNGIGFGPKDPGKNRDGSKPNWFDDTYPVREDYPVGGLS
                     SEASILEVLAAIKARVPYLFRYEVPVSEGSKILRLKMVPQTARDLACHAAAALGDGWQ
                     LMLFKNGFTLYKAVKSYEHGIQLHPPKR"
     gene            complement(7869..9203)
                     /locus_tag="PLP58_17235"
     CDS             complement(7869..9203)
                     /locus_tag="PLP58_17235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007415449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA cytosine methyltransferase"
                     /protein_id="HRJ10326.1"
                     /translation="MKTKSAPKKAPNPPRQAGRRAVPQKARQEKAGGSLREEARRAPV
                     SQLRSIELFAGAGGLALASANAGFSHDAVLEFNHDACETIRANQRRGYEVVRHWPLIE
                     GDVHDQSFTSWQGRIDLVSGGPPCQPFSIGGKHRAMADRRNLFPEAARVVRETRPRAF
                     VFENVKGLTRQSFAKYFGYIVLQLSFPDIVRRKDEEWLEHLSRLERIRTQGARPDLSY
                     KVVWRLLNAADYGVPQKRERVFFVGIRSDMEIPWSFPQPTHSRLALLHDQWVAGNYWE
                     RHRVARRARPEAPARLPFLPMEEMCLPSMAWPLPWLTVRDAIHDLGEPARSQTSAIHP
                     NHILQPGARSYPGHTGSPLDEPAKTLKAGDHGVPGGENMLRHPDGGVRYFSVRESCRL
                     QTFPDEYVFEGSWTENMRQLGNAVPVRLGEVVLRSVRAAFEKHDAASTPGLF"
BASE COUNT         1658 a         3301 c         2775 g         1630 t
ORIGIN      
        1 ccaccacagc cacccgcccc tggcactgcg cctggaggcc ctgcggcggg cttgatggaa
       61 caaagcgccc ctccccgccc acttccaatg cgctctatgc gacttcggtc agaaggtcgg
      121 cgaacagatc atactgcttg agcctgatct cgcgtttctg tgtgtgcgcg ggcatgctgg
      181 cctcgaaatt atagacaaga gcttcaacga cagaacggcg attttcgagc ttgcctcccg
      241 tgtggtagaa gtgatcgcgc gtgatcacat tgaacccgct ccagtaagcg ggtatcgcct
      301 cgttcttgcg gtagtcgttg tgatgccacg ttgagagaat gaatctggca ggtgtagccg
      361 tcagcaatcc ggataaacga cgctcatcct cctccgtcca gttgctgaag tagtccacat
      421 ggcgcccgag gtagggaggg tcgcagtaaa tcatgtcgtc agggcctgcc atcttgataa
      481 tcttctcaaa atccgccgtg atgaactgcc atccgggctg gatgatcttt gcggcatcag
      541 ccacttggtt gacgatcttg gtgacatacg ccggggcaaa gcgcccgggc tttttgcaga
      601 aggggatgtt ccacgctccc tttcgattga atctcatcat gccattgaag ccggctctgg
      661 agagaaagat caggtccagc gggttgtgct ctgagttgaa ccggtcccga agataccgga
      721 agtgcgcgta gccgtcttca tcagacgcgc tcaagatcgc tccttccttc tccaagtgtt
      781 tccggacact ccccgctgtg atctccccgg attggatggc ttggtagagg cggatgatgt
      841 gcggattgat gtctcccagg agagcctggg agcctccgca attcagagcg acaacgcccg
      901 tgcccaaaaa aggctcgatc cagcgtcctt ggaagacagg ggcaaggtct ctaatccaag
      961 gcaccagctt ggtcttgatg ccctggcttt tgagtggcgg cacgatgact ctcataggcc
     1021 cttcgatttg cgcggatttg tcttggtgcg cacgggaacg atcaactttt tgtccgcgcc
     1081 tttaaagtcc atgaagtccg ccagcgtcct gattggcact gacttgcctt cacgcagcat
     1141 ggttgcctgc ccgtgattca tccaatactc gtcaaaccac ttctcgccaa gtctggaaaa
     1201 gactccatgg ccgcgccgaa tgtcctcgat ccatgtgatc gccccgatgt tcgcggtgtt
     1261 gcccgagcct tgcttgtcgc tggccaactg ccacttctcg cagacgaaga attcgaagtc
     1321 acgcaccacg gatgtgatgg aacgcaagtc ttcgacgatg gccgccgatc cttcagcttt
     1381 ttgctttcgt gtgacatggc ccaattcctt cacggaaaaa gttcgcgtct catccagctc
     1441 atccgcgccc acccgggtat agatcacgcc aaggcagatg tgcgcggcgt agctggcgta
     1501 ggggaactgg atgttttttg tcgaggcgcg atccttgaaa taagcgccgt ggctcccaag
     1561 agtgaagcca ttgacgtgcc cagtgaactc aggatctcga tagctggtct tcaaatccac
     1621 cgcgaacttg aggcccgggt cagccttgct cacaaagctc aaatcgggat accagttctg
     1681 tttctccgcc agcacgatgg agtatccttg ctgcccggca aaagccagca gcttcgggaa
     1741 gatgtgaatc tccaaaatct tcgacacaat cttcgtgtcc gaactcaccg tgaagacatt
     1801 gcgataaata tcaataaagc ctttgaccga ccactggccg tcctcgtccg ccacatgatc
     1861 gcgcagctct gtgacgaact gggtcaaggc cgccatgaat gcttttttga gtgcagcgtg
     1921 cttcttcatg tcaccggatg atggagcgag acttcggagc tgtcgagccg aagtgaccgc
     1981 gcaagcggcg ggccgcagcc ccaccccctg gccgtctgcc tccaccacag ccacccgccc
     2041 ctggcactgc gcctggaggc cctgcagcgg gcttgagggg agcagtgcgt catcccgcac
     2101 atccagccag tcaggtccgt caggcctgcc cggtccatca ggccctccga gatcgcgaaa
     2161 actttccagt aacgccaagc ccgtttttcc gatagaacac tgggacactc cctccaagga
     2221 cagcgcacca tgcccgcgcc gccccataca gctgtcatga aaacgcgcac gcacctcctc
     2281 gcactcgccc tcctcctgcc cgccgcaacc gcgctcgcgg aggacgtcgt ctcgaatctc
     2341 gacaacgcct ccaccgcgca gatcgccctc tcttccgtgg gtgccagcgc cgcgcaggcc
     2401 ttcaccaccg ggccggaggc gcggaaactg gatcgcatca ttgtgcgcct gcgcggtttc
     2461 caggccgggc cgcacacagc gcgcgtgaag ctctggacag gctcgggcac gctgccggtc
     2521 acgctcgtcg aggacctcgg cacggtgccg aacatcccct ccggcaacga tggagagatg
     2581 acggtgctct ccaccacgaa tcccgacctg gcgcccaaca cccgccactg ggtctccgtg
     2641 agcgtctctg cgggggactt cgactggcgc aacacgagcg accccgccag cactggcacc
     2701 ggcgcctttg tgcagttgcg cgccacctcc ccctcgggca atgccggcac atggacggag
     2761 ccgggcttcg ccaccagcgc aaatttccag ctcatggagg tcaggcccat cgtcccgcag
     2821 accttcaccg tcaccaccac cgccgacgcc ggcggcggct ccctgcgcca ggccgtcgcc
     2881 gatgccaatg cgaatcccgg cgcggacatc atcgtcttcg actccgatct cgacggacag
     2941 accatcactc taaccaccgg ccagctcctg ctgacggatg acgtcaccat tgacgccagc
     3001 gccctgcccg gcggcatctc catctccggc aatgccgatg acaacggcac cctcgacccc
     3061 ggcgagtcgc gcatcttcga ggtggcgacg gggaaagtcg tcgtcctgcg cgccctgcgc
     3121 ctcgtcaagg gccaggcctc caccacggcg ggagacaaca gcgggcgagg cggggcgatt
     3181 cagacccgtg ggaatctcac cctcgaccgc tgcctcgtcg cggacagccg ggcgcggttc
     3241 ggcggcggga ttgacatcac cggcggcacg ctgacgatca cccagtccac cctctcgggc
     3301 aattccgcct cgcaaggcgg cgggattgta gtctttggcg gtttcgtaga actcacccaa
     3361 tgcaccctct cgggcaattc cgcgacgcgt ggcggcggga tctactgcat cggtggctcg
     3421 ctgacgatca cccagtccac cctctcgggc aattccgcct cggaaggcgg cgggattttc
     3481 tccaatcccg gttcgctcac catcacccgc agcatcgtcg ccgggaacct caccggcggg
     3541 gacattgacg gctccttcac cgacaacggc aacaacctca cctccggcga ccccctccta
     3601 gccccgctgg gcgactacgg cggtcccacg ccgaccatgc cgccgctgcc cggcagcccg
     3661 gccattgacg cgggcggcag caccacggag accaccgacc agcgcggctt cccccgcgtc
     3721 gccggcgcgg cggtggacat cggcgcnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3781 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3841 nnnnnnccac cgaccagcgc ggcttccccc gcgtcgccgg cgcggcggtg gacatcggcg
     3901 cggtggaggc gcaggccatc cccggcttcg accccgccgt ggtcaccagc ctcaccgaca
     3961 cctttgacgg cctgcccaac ggcgtctccc tgcgcgaggc ggtggccttt tccccgccgg
     4021 gcgccaccct caccttcgcg ccagggctcg acggccagac gatcaccctc ggcggcagcc
     4081 agatcctgct ggacaaaagc gtcaccattg acgccagcgc cttgcccggc ggcatctcca
     4141 tctccggcaa tgccaacgcc aacggcaccc tcgacgacgg cgagtcgcgc atcttcgagg
     4201 tggcgacggg gaaagtcgtc gtcctgcgcg ccctgcgcct catcaagggc caggcctcca
     4261 ccacagcggg gaacaacagc gcgctcggcg gggcgatcct aacggaaggg agtctcaacc
     4321 tcgaccgctg cttcgtctcg gagtgccggg cgctgttcgg cggtgggatt ggctgcgttg
     4381 gcggctcgct gacgatcacc cagtccaccc tctcgggcaa ttccgcctcg gatagcggcg
     4441 gcggaattgt agtccttggc ggttccgtcg agatcaccca gtccaccctc tcgggcaatt
     4501 ccgcatttag cggcggcggg atccacctca gtagcggttc cgtcgagatc acccagtcca
     4561 ctctctcggg caattccgcc tcggatagcg gcggcgggat tagatcctca ggtggttcct
     4621 ttctcacact cacccgcagc atcgtcgccg ggaacctcac gggcggggac attgacggct
     4681 ctttcaccga caacggcaac aacctcctct ccggcgaccc cctgctcgcc gctctcggcg
     4741 actacggcgg ccccacgccg acgatgcccc cgctccccgg cagcccggcc attgacgcgg
     4801 gtggagccgc ggcttccgcc ttccccaccg accagcgcgg cttcccccgc gtcgccggcg
     4861 cggcggtgga catcggcgcg gtggagtttg atctcgacat agacagcggc ctgcccggca
     4921 cgctggcctt tggctccgcc ctgaccaccg tgctggagga ggcgggcgcg ggcatggtgc
     4981 cggtggtgcg caccggcggc atcaccggca gcgtgagcgt gacggtgaac agcgcccccg
     5041 gcacggccac ggcggcggac tttgacgccg tcagcggcct ggtgctgacc ttcgcccagg
     5101 gagagcggca gaagctggtg cccgtgaccg tgctggccga cacgctcaag gagccgaccg
     5161 agttattcac cctgacgctg agcgccccca ccggcggcgc cgcgctcggc acgccgaacc
     5221 ccggcatcgt gcgcatcatt gactccgcgc tggacaaggc caagcccgcg ctcagcctgc
     5281 tcacgccgaa aaataacgag atcatcctcg aagccgccgg gaacgtggtg gccctcaccg
     5341 gcagcgccaa ggacaatatc ggcatcgagc gcgtggaaat ctcgcttgat gggggtgact
     5401 tccagcccgc cgcgctgaca cacgccgccg acttcaaaag ctgcaccttc gccctgccgc
     5461 tgaccgtcac ccccggcctg cgcaccgtgc tggtgcgcgc ggtggacacg cgcgggctgc
     5521 tctccgcccc cgtgaagcgc gccttcacct tccgcgtcgt gcgccagctc gcggtcaatg
     5581 tcagcggccc gcccggctcc ggcacggtga cgaagggttt cgaacccaat tcgcagcgct
     5641 tcgtcggcat cccgcacacg atcaccgccg cgccgaagcc cggctttgtc ttcactggct
     5701 ggacggccaa tgacttcacc ggcaccggcg tcaccaccgc cgcgcgggag ctgccgaagc
     5761 tcgccttcct catgcgggag gggctggtgc tcacggcgaa cttcctggcc aatcccttca
     5821 caccgcagat caccggcatc cacagcggcc tggcgctgcc ggacgccggc accacgcccg
     5881 gcgccggcac cgtggggctt ttcaccgcca ccgtcaccgg cacgggagcc ttcaccggca
     5941 gcctcatgct ggacggggcc aagctcccgg tcagcgggct gttcacccat caaggccagt
     6001 cccgttttgg caaaacccgc agccccgcgc tggtgctcct gcgcaaaggc aaacccgcgc
     6061 tcacgatcac cctggccctg gacatgagcg gcgccaccgg ccgcatcacc ggcaccgtca
     6121 cccaggaggg tggcgccacg tccgacatca ccgccgaccg cgcccactac agcagcaaga
     6181 gcccggcgga ccccgccctg gccggggcga aaggccagcg ttacaatctc atcctgcccg
     6241 ccaaagcaca gacacccgcc cgcgccctga acctctaccc gcagggcacc ggcatcggcg
     6301 gcatgaccgt gaagccggac ggcaagacca gcttcaccct gcttttggcc gacggcacca
     6361 agctcaccgc cgccgccgcg ctctcgaaaa acgacgactg cccgctcttc gcccagctct
     6421 acggcaagct cggcctgctc gccgcccagg tcacgctcgc ccccgcccag cccgccaccg
     6481 acgccaccgg gccggacagc gtgtggttcc gcccggccca gcccaaggcc acctcctacc
     6541 gcctcggctg gccgcagggc gtgagcgtgg acctcttcgg cagccagttc cacatccccc
     6601 ccgccgcgcc gcccgccagc gtcttccccg gcctcggcgc ggtggacctg atcaatggca
     6661 acgccgccct caccttcacc ggcggcctgc tcgtcatctc ccccagcacg ctgggcctca
     6721 acatagaccc cgccaacaaa gtgaccaacg cccccgccgc cgacaagacc ttcaccgccg
     6781 cgctcaccaa gaccaccggc gagtggaaag gcgtcttcac ccacagcgac ggcaaaaaac
     6841 ccgcctggca ggccgccact ttccagaaag ccggcgccca ccaaggcggc cacggcttct
     6901 tcctcagcct gcccgccaaa cccgccgacg gcctcgccga aagcggcgcc gtgcgcatcc
     6961 aggcgaggta gccgctggcg cagagtagcc gccgccgcaa ggcggtgggg gagttttgcc
     7021 ccgcgcggga gggcatctgc ggcgctgtgc aattgcaccc gtcaaaaagt gctgaaaggc
     7081 gtgccaaggc ccatgcgagg gcacggcatc ccaaaactcg ccgacaattc accgcttggg
     7141 cggatgcagt tgaatgccgt gctcgtagct cttgacggct ttgtacaagg tgaagccgtt
     7201 cttgaaaagc atgagctgcc atccatcacc caaggcagcc gcagcatggc aggcaaggtc
     7261 gcgtgcagtt tgaggcacca tcttcaatcg caatattttg gagccttcgg agacgggcac
     7321 ttcataacgg aaaagataag gaacgcgggc ttttatggca gccaaaacct ccaggatgct
     7381 ggcttccgat gacagcccgc caaccggata gtcttcccgc acaggataag tgtcgtcgaa
     7441 ccagttgggc ttgctcccgt cacgattctt tcccggatct ttggggccga atccgatgcc
     7501 attccaacgg cattcaccaa gctttttgaa atgcccgatg agcagatcct cattggcgct
     7561 ggtgctccag ttttcatcca gcaacaaagc tttgaagccc acatgactca tgtccatgcc
     7621 gcttctcccg gagagtttgg ccagatgttg aaacaacctc tccgccgtgt tttccgcctt
     7681 gcctgcgtat gcagcggcgc cttcatgata aatcacgtaa acgccttggc aggttttact
     7741 gcggctctga aacgcttgaa gctcgcgcaa ggcggattca ttgagaggtg ttgccgtcaa
     7801 agcggcaagt cgttcgacga gctgctcagt taccgctcgt ggcagactga agtggaagta
     7861 ttcaaacatc aaaaaagccc cggtgtgctg gcggcgtcat gtttctcgaa ggcggcgcgc
     7921 acgctgcgca gcaccacctc gcccaggcgc actggcacgg cattgccgag ctggcgcatg
     7981 ttttccgtcc agcttccctc aaagacatac tcgtccggga aggtttgcag ccggcatgac
     8041 tcgcgcacgc tgaagtagcg caccccgcca tcgggatggc gcagcatgtt ttccccgccg
     8101 ggcacgccgt gatccccggc cttcaaagtt ttggccggct cgtccagcgg gctgcccgtg
     8161 tggccggggt aggagcgcgc gccgggctgg aggatgtggt tggggtggat ggctgatgtt
     8221 tgggaccggg caggctcgcc caggtcatga atcgcatccc gcaccgtcag ccacggcagg
     8281 ggccaggcca tggagggcag gcacatttcc tccatcggca gaaatggcag gcgcgccgga
     8341 gcctccgggc gcgcgcgcct ggcgactcga tgcctctccc agtaattccc cgccacccat
     8401 tgatcatgca gcagcgccag acgcgaatgc gtgggctggg ggaatgacca cgggatttcc
     8461 atgtcggagc ggatgccgac gaagaacacg cgctcgcgct tttgcggcac gccgtagtcc
     8521 gccgcattca gcagccgcca tacgactttg taactcaagt ccggcctggc gccctgcgtg
     8581 cggatgcgtt ccagccggga aagatgctcg agccactcct catccttccg gcgcacgatg
     8641 tctggaaagc tgagctggag cacgatgtag ccgaagtact tggcgaagga ctggcgggtc
     8701 aggcctttga cattttcaaa cacaaaggcc ctgggccgcg tctcccgcac cacgcgcgcc
     8761 gcctcgggga aaagattccg ccggtcggcc atggcgcggt gcttgccgcc gatggagaag
     8821 ggctggcagg gcgggccgcc ggagacgagg tcgatgcgcc cctgccagga ggtgaagctc
     8881 tggtcatgca catccccctc gatgagcggc cagtggcgca ccacctcgta accccggcgc
     8941 tggttcgcgc ggatggtctc gcaggcgtcg tggttgaact ccagcaccgc gtcatggctg
     9001 aagcccgcgt tggccgacgc cagcgccagc ccgcccgcgc ctgcaaacag ctcaatggag
     9061 cgcaactggc tgacgggcgc gcgcctggct tcctcacgca gcgagccgcc cgctttttcc
     9121 tggcgcgctt tttggggaac cgcccttctc ccggcttggc gcggcgggtt gggggctttc
     9181 tttggagcac tcttcgtttt catgggcttg ccggggattg ggctatgaaa cacggcctct
     9241 ggcaaggcaa tttctccgcc gggaagcgga tgccggactt tatttgccgc tcacgctgtg
     9301 ctgaagctgc ccgccccgag cgtggtcatt accggggcgg cggatgccgg cgggcggttc
     9361 caaaaatggc ggctttttac cggtgccggc ggcgggtttc cggccagcat ggcgggcgag
     9421 gccactccga ccatgaagcc gcacgccagc gcgcccgccc cgcc
//
LOCUS       DAOMYJ010000200         9453 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Prosthecobacter sp. isolate AMR_MDS_5629
            NODE_2418_length_9453_cov_21.659289, whole genome shotgun sequence.
ACCESSION   DAOMYJ010000200 DAOMYJ010000000
VERSION     DAOMYJ010000200.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867850
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prosthecobacter sp. (wastewater metagenome)
  ORGANISM  Prosthecobacter sp.
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Verrucomicrobiaceae; Prosthecobacter.
REFERENCE   1  (bases 1 to 9453)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9453)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.30
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:34:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,742
            CDSs (total)                      :: 4,684
            Genes (coding)                    :: 4,676
            CDSs (with protein)               :: 4,676
            Genes (RNA)                       :: 58
            tRNAs                             :: 55
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9453
                     /organism="Prosthecobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2418_length_9453_cov_21.659289"
                     /isolate="AMR_MDS_5629"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1965333"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..182)
                     /locus_tag="PLP58_17240"
     CDS             complement(<1..182)
                     /locus_tag="PLP58_17240"
                     /inference="COORDINATES: protein motif:HMM:NF015791.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate epimerase"
                     /protein_id="HRJ10327.1"
                     /translation="MPLTCREMQDGEARAFEAGVDSADLMEAAGQGIARVIRQFFPAP
                     GTAVLFLGGGNNAGDAL"
     gene            complement(186..824)
                     /locus_tag="PLP58_17245"
     CDS             complement(186..824)
                     /locus_tag="PLP58_17245"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: protein motif:HMM:NF013670.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HRJ10328.1"
                     /translation="MAEIFSHRSRRPGKAGKLAGLAFVAVVFLTGAARAFDTVILDAG
                     HGDHDRGASIGYVYEKHLCLDTARRVERLLKQAGLKVVLTRSTDEFVTLQNRADMGNS
                     RRNAIFVSIHYNYSRGGSGHGVETFYHFEKSYLLAAYIQAYLLKSTGLANRGVKHASY
                     FVIRKTTRNPAVLVECGFVSNSAERQKMMDGAFRAKIAEGIAQGIVGYKRSR"
     gene            1030..7800
                     /locus_tag="PLP58_17250"
     CDS             1030..7800
                     /locus_tag="PLP58_17250"
                     /inference="COORDINATES: protein motif:HMM:NF025006.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase regulatory-like
                     domain-containing protein"
                     /protein_id="HRJ10329.1"
                     /translation="MKIRAFMQGGLVIAMLAVIAARTDAATLQGQVTSATTGEPLAGA
                     DVMVDAEPADGTPEFETVTDLFGFYEIRDVPAAAQRLRAALGGHTPVERAVAVAGLTM
                     NENFALTPLPGVTPVEIHVEVADAASNWKIPDIPVEIIRYDSATDLTPDPPVTVKTDA
                     TGNFSFYGITAGYYTFAFNTGAQRRPGWESYPKAGGHTAKMAVSSPHLAGLMLKHVPQ
                     TIKLRVEGMDPTTEQNGPLKNMVVELTGVSPDDHELVLMPPREMATDDAGEVSFHNLA
                     PIHWRARIKRPGYELTDTFLEPAAVTDDLPGRQPGAAEVLQAVIEPTALLLTLKSAYD
                     TNEFYEGIEVRLDGVARTNAEGIRRQLDTGVTPEERLFSNLLPGRYEVRVSDRFEPDD
                     PLTTPAPHFTAEETVELVDGLTTEEELLLEVRRAIVRARLFKADSMGEMTTGGGNDFV
                     LRRPIYQPHEAEVLMGMDGDHVTGGADENGDLVFVVNPGLYGFAVPSMTDYLGSHILV
                     QHDMAVDPRRQGWPVISGASSSPAPPDAALGDPLVINSDREYRIDLYAHKKGTTLALD
                     VVLTGDNPTQDIVTSIPVPFSAPQTAKFVELLAVPPVFEITEAGSSQTITGSLLSRGG
                     RLVVEFANVMPGTWTWTGTHPRFDIEAFPLLPGSTPETVTLPGLPEYPGANYPAGGPP
                     GAFFFYNPVLMYQTWPTGVPADAAYKASPPMTLRRKVWDEELDPPQYVDAGTDSAPHF
                     DFIRPAYAGGALFAAARRPLGSFDFWKRDPSGLWFAGSASTGPEDLTIFVGGPSNNVT
                     PHPPVMTAAITLKAVSREAPDVEIPGIKFNLGGSIFDAGSISVPGQYTGPLTLTTATD
                     PAGVWLYKRHGVITSGVFNNAVLIVAEMGRALNLSGTVKVDNAAAEPIPGAKIRLRDR
                     FGDARFPAKVTDDNGAFVMPSTLETPAFFVDVSAPGFKPRRLRFGGADPAIGLNGSLV
                     LDLRLEPLPAPAVEAEINRYGLFLSNVSFSSKGPLGELGRAVDELTATWTATATPAAE
                     INFEELNFDNADGTPGGSTQRTLKDGIALVAIVDPRLFPRESSDAAGTGGEGAGEEGM
                     FDYMPDPDPAKVLARRAWWDSITTQSYKDGTGAPLEGFHIISRIATSMMTDPMTHVTT
                     ASGQLKLWTLPPGRFSPVIVVETKHGALSFHQITYTEADSAKTLRALRPPAWMAAIAD
                     AMALTKKFAGSPLLQKRALDKHMSGKFKPKRITNGVISVGLPGRPEEGYLKYAYNGNT
                     QWNEGQSVSAEGFLGMVTDTIGGALEVALDVVVDGTDGSVLFEARGTATPDAVEASLF
                     KPARLTERQKQRIKQKRHFIGSASFSNTGIFDPEAEPLELLTKSRFLFGAAIETRYDL
                     KPHIKLIPKVGPLFRVADRLSGKKFQVDALARAAAGFDVVSTWTTRRPQIAAAGSGVT
                     GSFPHPEKGHYLGGREVAQPGVPATAFSQSTSLLFHLGIGIEAAIGELAGASVNLSLQ
                     GSKVKIPIAEGEDLPALQLTFNPDGYWPVFKEVRGEIRATFQAFADLVLTRLEKISFF
                     DIPFSYQFSTVSRFDLVPLSVTFTSRSLADEPPATWLGASPQLARGILLSARDTHRIA
                     AGTPDLMVFPDIDPMTGRMLLRAAVRVNETTWGTPVTLGDEDGIVAADAARLSNGTLL
                     AVWTSITAADLASPGAGSTIRFSTSTNGGVTWSAPATAAVLAGVADTLHLETSGTFNS
                     LVYLMREGFPGSPLQSISGMTYSTVSGWSAPATLAASQEILSLDAAGVSGGVTVAFTN
                     AADELKVFSWSGGAPGAIATNTTPVNPGVAVIESGGLAVILSLPQAGGIQTRSFFFGS
                     LLAGPLVYADETPDGISGAAVGGSPGRILIAWTDGPAGALRYALLNAGTFAAVSQEPL
                     EVTRNSVGRYTLADVVQSPSGHATVFTRFHAPGAQPELRQFIISRTDGLVFTDRDGDG
                     INDIDELLIVDTDPNDDIHTIDDVTSGPGGSDFDSDGVSDADEIAAGTDPADPRSRPL
                     PPGVNLSVTAPTAQEFGLIPGQFQITRSDATQAVDVLFSISGTTTEGDDYLLNAASPL
                     HFEADQATLSVDVLPLADSLPEGAETIILTLLPDAAYALGAQTSGLVTLHDRPSDAWL
                     RVHFPGNPESVSFDGDTDGDGISEAMEFGLALDPNNAQVGGLPIAGRTADGNFITLRY
                     TRPIESDLFYTVQVSDDGVIWRSGGLHTTEVSREHHDNGTETVTVRGNTPFGAVPRQL
                     MRLEVRRE"
     gene            7925..8962
                     /gene="tdh"
                     /locus_tag="PLP58_17255"
     CDS             7925..8962
                     /gene="tdh"
                     /locus_tag="PLP58_17255"
                     /EC_number="1.1.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009959141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008743 - L-threonine 3-dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006567 - threonine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine 3-dehydrogenase"
                     /protein_id="HRJ10330.1"
                     /translation="MKALVKAHAERGLWLQDVPEPQVGINDVLIKIRKTGICGTDLHI
                     YKWDAWAQKTIPVPMVVGHEFVGEVVAIGSNVNDFHPGEIVSAEGHVVCGRCRNCLAG
                     RRHLCKDTVGIGVNRTGAFAEYISVPMTNVWHHREGVDEEVASIFDPFGNAVHTALAF
                     ECMGEDVLITGAGPIGIMAIPVVKHAGARHVVITDVNEYRLDLAKRMGATRAVNVKHE
                     TIADVQKQLGMKEGFDVGLEMSGNAAAFRDMIDNMCHGGKIAMLGIPSEQIAIDWNKV
                     IFNMLTIHGIYGREMYETWYQMSVMLESGVNIAPVITHRFHYTEFEKGFAAMESGNCG
                     KVVLDWTNGMP"
     gene            8959..>9453
                     /locus_tag="PLP58_17260"
     CDS             8959..>9453
                     /locus_tag="PLP58_17260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009959143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HRJ10331.1"
                     /translation="MNTTFKDHLTAQLDAIRAAGTYKRERVLSTPQGTLVRANGGAAV
                     LNMCANNYLGLAQHPKVRQAAHEALEHWGYGLASVRFICGTQGLHKELEASLTDFLGT
                     EDTILYGSCFDANGGLFETILGPEDAVISDELNHASIIDGVRLCKAQRYRYKNCDMAD
                     LEAQL"
BASE COUNT         1805 a         3059 c         2914 g         1675 t
ORIGIN      
        1 agcgcgtcgc ccgcgttgtt gccgccgcca agaaaaagca ccgcggttcc cggcgcggga
       61 aagaactggc ggatcacccg cgcgatcccc tgccccgccg cctccatcaa gtccgccgaa
      121 tccactccgg cctcaaaggc gcgcgcctct ccatcctgca tctcccggca tgtgagtggc
      181 atggttcagc gcgagcgttt gtagccgact atgccctggg cgatgccctc ggcgatttta
      241 gcccggaaag cgccgtccat catcttctgg cgctcggcgg agttgctgac gaagccgcac
      301 tccacgagca ccgcggggtt gcgggtggtc ttgcggatga cgaaatagct ggcgtgcttc
      361 acgccgcgat tggccaggcc tgtggacttc agcagataag cctggatgta ggcggcgagc
      421 aggtagcttt tttcaaagtg ataaaaggtc tcgacgccat gcccggagcc gccccggctg
      481 tagttgtagt ggatgctgac gaagatggcg ttgcggcggc tgttgcccat gtccgcgcgg
      541 ttttgcagtg tgacgaactc gtccgtcgag cgagtcagca cgaccttcag cccggcctgc
      601 ttgagcaggc gctccacccg ccgcgcggtg tccaggcaga ggtgcttctc atacacatag
      661 cctatggagg ctccgcgatc atggtcgccg tggcccgcgt ccaggatgac agtgtcaaaa
      721 gcacgcgccg ctccagtcag gaaaaccacc gccacaaagg ccagtccggc tagtttcccg
      781 gctttgccgg ggcgtctgga gcggtgtgaa aaaatttcag ccattgagaa acggagcgct
      841 gctgtgctgc cgcgccagta agcccctgcg caaggcgggg tcaagcgttt tggctgcgga
      901 gtggcgccag catccgttga tttactgcct ggagccgccg caggagccat tgccagcctg
      961 caagcctcgc ctgatccggt gtgaaaaatt gttcttccat caatcggcaa gattggttaa
     1021 tctcaatgca tgaaaatccg cgcattcatg cagggcgggc tggtgattgc catgctggcg
     1081 gtcattgccg cgcggacgga cgcggccacc ctgcaaggcc aggtgacaag cgccacgacc
     1141 ggcgagccgc tggcaggggc ggatgtcatg gtggacgcgg aaccggcgga cgggacgccg
     1201 gagttcgaga cggtgacgga cttgttcggc ttttacgaaa tcagggacgt gcctgccgcc
     1261 gcgcaaaggc tgcgggcggc gctgggcggc cacacgccgg tggagcgtgc ggtggcggtg
     1321 gcagggctga cgatgaacga aaacttcgcg ctgacgccgc taccaggcgt gacgccggtg
     1381 gaaattcatg tcgaagtggc ggacgcggcc tcgaactgga aaattccgga cattccggtg
     1441 gagatcatcc gctatgacag cgccaccgac ctgacaccgg acccgcccgt caccgtgaaa
     1501 acggacgcga cgggcaactt ctcgttttat ggcatcactg cgggttatta cacattcgct
     1561 ttcaacacgg gggcgcagcg ccgcccaggc tgggagagct acccaaaggc aggcgggcac
     1621 acggcgaaga tggcggtgtc gagtccgcat ctggcgggcc tgatgctcaa acatgtgccg
     1681 cagaccatca aactgcgcgt ggaaggcatg gacccaacca cggagcaaaa cggcccgcta
     1741 aagaacatgg tcgtggagtt gaccggggtg agtccggacg atcatgagct ggtgctgatg
     1801 ccaccgcggg agatggcgac cgatgacgcg ggcgaggtca gctttcacaa cctggcgccc
     1861 atccactggc gcgcgcgcat caagcggccg ggctatgagt tgacggacac gtttctggag
     1921 cctgcggcgg tgacggatga ccttcccggc aggcagcctg gagccgccga ggtgctgcag
     1981 gcggtgatcg agccgaccgc gctgctgctg acgttgaagt cggcctacga cacgaacgag
     2041 ttttacgagg gcatcgaggt gaggctcgac ggcgtcgcgc ggacaaacgc ggagggcatc
     2101 cgccgccagt tggacacagg cgtgacgccg gaggagaggc tgttttcgaa tctgctgccc
     2161 ggccggtatg aggtgcgtgt gagcgaccgc ttcgaaccgg acgatccact aaccactccc
     2221 gcgccgcatt tcaccgccga ggagacagtg gagttggtgg acggtctcac cacggaggag
     2281 gaactgctgc tggaggtgcg ccgcgccatc gtgagggcca ggctgttcaa ggcggactcg
     2341 atgggcgaga tgacgaccgg cggcggcaat gacttcgtgc tgcgccgtcc gatctaccag
     2401 cctcacgaag cggaggtgct catgggcatg gatggagatc atgtgacagg tggggcggat
     2461 gaaaacggcg acctggtctt tgtcgtgaat cccggccttt acgggttcgc cgtgccctcg
     2521 atgacagatt acctcggcag ccacattctc gtgcaacacg acatggctgt tgacccgcgc
     2581 cgccagggct ggcctgtgat cagcggggcc tcatcctcgc cggctcctcc ggacgctgcg
     2641 ctgggagacc cgctagtcat caactcggac cgcgaatatc gcatagacct ttatgcgcac
     2701 aaaaaaggaa ccacgctcgc gctcgatgtg gtgctcaccg gagacaaccc cacccaggat
     2761 attgtgacca gcatccccgt gccgttcagc gcgccgcaga cggcgaagtt cgtggagttg
     2821 ctggcggtgc cgccggtctt cgagatcacc gaagcgggca gcagccagac catcacgggc
     2881 agccttctta gccgcggcgg ccggctggtg gtggagttcg ccaatgtcat gcccggcacc
     2941 tggacatgga cgggcacgca tccgcgtttc gacatcgagg cgtttcccct tctgcccggc
     3001 agcacgccgg agacggtgac actgccgggg ctgccggagt atcccggagc gaactacccg
     3061 gcgggaggcc cgccaggcgc tttctttttt tacaatccgg tgctcatgta ccagacatgg
     3121 cccaccggcg tgccggctga cgcggcttac aaggcctcgc caccgatgac cctgcgccgg
     3181 aaagtttggg acgaggagct tgatcctccc cagtatgtgg acgccgggac cgattccgcc
     3241 ccgcactttg acttcatacg ccccgcctat gctggcggag cactgttcgc ggcggcgcgg
     3301 cggcccttgg gcagctttga tttttggaag cgcgatcctt ccggcctgtg gttcgcaggc
     3361 agcgctagca ctggacctga agacctgacc attttcgtcg ggggaccatc aaacaatgtg
     3421 actccgcatc cacccgtgat gacagccgca atcaccctca aggcagtgag ccgcgaggct
     3481 ccggacgtgg aaatacccgg aatcaaattc aacctcggcg gctccatctt cgacgcgggc
     3541 agcatcagcg tgcccggcca gtacaccggc cctctcacac tcaccacggc caccgacccc
     3601 gccggcgtgt ggctttacaa aaggcacggc gtcatcacct cgggcgtgtt caacaacgcc
     3661 gtgcttattg tggccgagat ggggcgcgcg ctcaacctct caggcaccgt caaagtggac
     3721 aatgccgccg ccgaaccgat cccaggcgcc aaaatccgcc tgcgggaccg ctttggcgac
     3781 gccagattcc ccgccaaagt cacagacgac aacggcgcct ttgtcatgcc gtccactttg
     3841 gaaaccccgg ctttttttgt ggatgtcagc gcgcccggat tcaaacccag gcggctgcgt
     3901 tttggcggcg cggaccctgc catcggactc aacggcagcc tcgtgctcga cctgcggctc
     3961 gaacccctgc ccgctcccgc agtggaggct gagatcaacc gctacggcct gtttttgagc
     4021 aatgtgtcct tttcctccaa aggccccctg ggagagctgg gacgcgccgt ggatgaactg
     4081 acggcgacct ggacggcgac cgccactccc gccgcggaga tcaactttga ggaattgaat
     4141 ttcgacaacg ccgacggcac tcccggcggc agcacccagc gcacgctcaa ggacggcatc
     4201 gctctggtgg cgattgtaga cccgaggctg ttcccgcgcg aaagctcgga tgccgccggc
     4261 accggaggcg agggagccgg cgaggagggc atgttcgact acatgcccga tcccgacccg
     4321 gcaaaggtgc tggcgcgaag ggcctggtgg gactcaatca ccacgcagag ctataaagac
     4381 gggaccggcg cgccgcttga gggcttccac atcatttcaa ggatcgccac cagcatgatg
     4441 accgatccaa tgacgcatgt caccacagcc agcgggcagt tgaagctttg gacactgccc
     4501 ccgggcaggt tttcacctgt catcgtggtg gagaccaagc acggcgcgct gagctttcac
     4561 cagatcacct acacagaggc ggacagcgcc aaaactctgc gcgccctccg gcctcccgcc
     4621 tggatggcgg ccatcgcgga tgccatggca ctgacgaaaa aattcgccgg ttcgccgctg
     4681 ctgcaaaagc gcgcgctcga caagcacatg tccggcaagt tcaaacccaa gcgcatcacc
     4741 aacggcgtca tcagcgtggg cctgcccggc aggccggagg agggctacct caaatacgcc
     4801 tataatggca acacccagtg gaatgaaggg cagagtgtct cagcggaggg ttttctcggc
     4861 atggtcaccg acaccatcgg tggcgcgctg gaggtggcgc tcgatgtcgt tgtggacggg
     4921 acagacggca gcgtgctctt tgaagcgcgc ggcacggcca cacccgacgc cgtggaggcc
     4981 agcctgttca aacccgcgcg tctcaccgag cggcaaaagc aacgcatcaa acagaagcgc
     5041 cacttcatcg gctccgccag cttttccaat accggcatct tcgaccccga ggcggagccc
     5101 ctggagcttc tcaccaagtc gcgcttcctg ttcggcgccg cgatcgagac gcgctacgat
     5161 ctcaagccgc acatcaagct catcccaaaa gtgggtccct tgttccgagt ggcggacagg
     5221 ttgtccggaa aaaaattcca ggtggacgcc ctcgcgcgag cggcggcggg attcgacgtg
     5281 gtcagcacct ggaccacgcg gcgtccgcaa atcgccgcag caggcagcgg cgtcacggga
     5341 tcatttcctc atccggaaaa gggtcattat ctcggagggc gcgaggtggc gcagcccggc
     5401 gttccggcta cggcgttcag ccagtccacc agcctgcttt tccatctcgg cattggcatt
     5461 gaggcagcca tcggcgaact cgccggtgcc tcggtcaacc tttcccttca aggcagcaaa
     5521 gtgaagattc ccatcgccga gggggaggat ctgcccgcgc tgcaactgac cttcaacccc
     5581 gacggttact ggcccgtctt caaggaagtg cgcggcgaga tacgcgccac cttccaggcc
     5641 tttgccgacc ttgtgctgac acggttggag aaaatatcgt tcttcgacat tcctttctcc
     5701 taccagttca gcacagtgtc gcgcttcgat ctcgtgccgt tgagcgtcac attcacttcc
     5761 cgctcgctgg cggatgagcc gcccgccacc tggctgggtg cctcccccca gcttgcccgc
     5821 ggtattctcc tctccgcgcg cgacacgcac cgcatcgccg ccggaacccc cgacctcatg
     5881 gtcttccctg acattgaccc aatgaccggg cggatgctgc tgcgcgcagc cgtccgggtc
     5941 aatgaaacga cttggggaac gccagtcacc cttggcgatg aggacggcat cgtggccgcc
     6001 gatgccgccc ggctttccaa cggcactctc ctggcggtgt ggacttccat tacggccgcg
     6061 gacctcgctt cgccgggcgc aggttcgacg atccgctttt ccaccagcac aaacggcggc
     6121 gtcacctggt cagcgcctgc cacggcagcc gtgctcgccg gagtggctga caccctccac
     6181 ctggagacca gcggcacatt caactcgctg gtgtatctga tgcgggaagg ctttccaggg
     6241 tcacctttgc agtccatctc cggcatgacc tactccacgg tctcaggctg gtcagcgccc
     6301 gcgacactgg cggcgtcaca ggagatactg tcccttgatg ccgccggagt ctcaggtggt
     6361 gtgacggtgg ctttcaccaa tgcggcggat gaattgaaag tcttctcctg gtccggcggc
     6421 gcgccgggcg ccatcgccac caacaccaca ccggtgaatc ctggtgtcgc ggtcattgag
     6481 tcaggcggcc ttgctgtgat cctgtctttg ccccaggcgg gtggaatcca gacacgcagt
     6541 ttctttttcg gcagcctgct cgccggcccc ttggtttatg ccgacgagac gcccgacggc
     6601 atcagcgggg cggctgtcgg aggcagtccg ggccggattc tcatcgcctg gacggacggc
     6661 ccggctggtg ccttgcgtta tgcgctgctg aatgcgggca cttttgccgc cgtcagccaa
     6721 gagccgctgg aagtgacgcg gaactcggtg ggccggtaca cactggccga tgtggtgcaa
     6781 agcccgtcgg ggcacgccac cgtcttcact cgctttcatg ctcccggcgc ccagcccgag
     6841 ctgcgccagt tcatcatctc gcgcacggac ggactcgtct tcaccgaccg cgatggcgac
     6901 ggcatcaatg acattgacga gctgctcatc gtggacaccg accccaacga cgatatccac
     6961 accattgatg atgtcaccag cggccctggc ggctcggatt tcgacagcga cggcgtcagc
     7021 gatgccgatg aaatcgcggc cggcaccgat cccgcagatc cgcgcagccg ccccctgccg
     7081 ccgggtgtga acctctcggt cacagcgccc accgcgcagg agttcggcct catcccgggc
     7141 cagttccaga tcacccggtc cgatgccacg caggcggtgg acgtgctctt ttccatcagc
     7201 ggcaccacca ccgagggcga tgattatttg ctcaacgccg ccagtcccct gcatttcgag
     7261 gcggatcaag ccaccctcag cgtggatgtg ctgccgctgg ccgactcgct gcctgaaggc
     7321 gcggaaacca tcatcctcac cctgctgccg gatgccgcct acgctctcgg cgcgcaaacc
     7381 tcagggctgg tcaccttgca cgaccgtccc agcgacgcat ggctgcgcgt ccattttccg
     7441 ggcaaccccg aatccgtttc ttttgacggc gacaccgacg gcgacggcat cagcgaggcg
     7501 atggaattcg gactcgccct tgatcccaac aatgcccagg tcggcggcct gccaatcgcg
     7561 ggcaggacag cggacgggaa cttcatcacc ctgcgctaca ctcgcccgat cgagtcggac
     7621 ctgttttaca cagtccaggt ctccgacgac ggagtcatct ggcgaagcgg cggcctgcac
     7681 acaacggagg tgtcccgtga acatcacgac aacggcaccg aaaccgtgac cgtgcgcggc
     7741 aacacaccct tcggggcggt gccgcggcag ttgatgcggc tggaggtgcg gcgcgagtaa
     7801 actggaggcg ttcatggcaa ccccgacgaa tcgaatcctg tatattggat tatatttcga
     7861 tattttggaa ttcctgcttg cagccctcga aggactcgcc attcatggcc gggttcaaca
     7921 caccatgaaa gcactcgtca aagcgcacgc cgaacgcggt ctctggctcc aggatgtccc
     7981 ggagccgcag gtcggcatca atgatgtcct catcaagatc cgcaaaaccg gcatctgcgg
     8041 cacggacctc catatttata aatgggacgc ctgggcgcag aagacgattc ccgttccgat
     8101 ggtcgtcggg catgagttcg tcggcgaggt cgtcgccatc ggcagcaatg tgaatgactt
     8161 ccatcccggc gaaatcgtca gcgccgaggg ccacgtcgtc tgcgggcgat gccgcaactg
     8221 cctcgccgga cgccgtcact tatgcaaaga caccgtcggc atcggcgtga accgcaccgg
     8281 cgctttcgcc gagtacatca gcgtgccgat gacgaacgtc tggcatcatc gcgagggcgt
     8341 ggacgaggag gtggcgagca ttttcgatcc cttcggcaac gccgttcaca ccgcgctggc
     8401 ctttgaatgt atgggcgagg atgttttgat caccggagcc ggccccatcg gcatcatggc
     8461 cattcccgtc gtcaaacacg cgggcgcacg tcacgtcgtc atcaccgatg tgaatgagta
     8521 tcgcctcgat ttggcgaaaa ggatgggcgc gacgcgtgcg gtgaatgtga agcacgaaac
     8581 catcgccgat gtgcagaagc agctcggcat gaaggaaggc ttcgacgtgg gcctggagat
     8641 gagcggcaac gccgccgcct tccgcgacat gattgataac atgtgccacg gcggcaagat
     8701 cgccatgctc ggcatcccca gcgagcaaat cgccattgat tggaacaagg tcatcttcaa
     8761 catgctcacc atccacggca tctacggccg cgagatgtat gagacctggt atcaaatgag
     8821 cgtcatgctt gaaagcggcg tgaacatcgc ccccgtcatc acccaccgct tccactacac
     8881 cgagttcgaa aagggcttcg ccgccatgga aagcggcaac tgcggcaagg tggtgctgga
     8941 ttggacgaat ggaatgccat gaacacgact ttcaaagacc atctgactgc ccaactcgac
     9001 gccatccgcg ccgcaggcac ttacaagcgc gagcgcgtgc tcagcacgcc gcaaggcacg
     9061 ctcgtccgcg cgaacggcgg cgctgcggtg ctcaacatgt gcgcgaacaa ctacctcggc
     9121 ctcgcgcagc acccgaaggt gcgtcaggcc gcgcatgagg cgctggagca ctggggctac
     9181 ggcctcgcaa gcgtgcgctt catctgcggc acgcagggtt tgcacaagga actggaggcc
     9241 agtttgaccg acttcctcgg cacggaggac acgatcctct acggctcctg cttcgacgcg
     9301 aacggcggcc tgtttgagac gatcctcgga cccgaagacg ccgtcatcag cgatgaactc
     9361 aaccacgcct cgatcataga cggcgtgcgc ttgtgcaaag cgcagcgcta ccgctacaaa
     9421 aactgcgaca tggccgatct ggaggcccaa ctc
//
LOCUS       DAOMYJ010000201         9407 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Prosthecobacter sp. isolate AMR_MDS_5629
            NODE_2438_length_9407_cov_19.198460, whole genome shotgun sequence.
ACCESSION   DAOMYJ010000201 DAOMYJ010000000
VERSION     DAOMYJ010000201.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867850
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prosthecobacter sp. (wastewater metagenome)
  ORGANISM  Prosthecobacter sp.
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Verrucomicrobiaceae; Prosthecobacter.
REFERENCE   1  (bases 1 to 9407)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9407)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.30
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:34:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,742
            CDSs (total)                      :: 4,684
            Genes (coding)                    :: 4,676
            CDSs (with protein)               :: 4,676
            Genes (RNA)                       :: 58
            tRNAs                             :: 55
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9407
                     /organism="Prosthecobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2438_length_9407_cov_19.198460"
                     /isolate="AMR_MDS_5629"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1965333"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            53..1324
                     /locus_tag="PLP58_17265"
     CDS             53..1324
                     /locus_tag="PLP58_17265"
                     /inference="COORDINATES: protein motif:HMM:NF013718.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016311 - dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dual specificity protein phosphatase family
                     protein"
                     /protein_id="HRJ10332.1"
                     /translation="MHTTEPLRPAREPRLLWPALWRLALAGAVFVGVYGGCNHFTATR
                     GDVPVMMFEWERNMPFIKEMVVPYWSLDVFFCGAFFLCRSRLELNLLTRRLVAVTLGS
                     GAFFLLFPLQLGLPRPEPEGWTAPLFHALYANDMPYNLAPSLHISLRSLVWVFYGAHL
                     RGRARVAVKIWFILIGASTLLVWQHHLVDVAGGFVMGWAVAALIPDPRQPGTRNPSLK
                     HARRYGTGALACAGLAFVSVVFVWPAMACGIMALAYATGLSRLLGKENGTLSPSAEWC
                     LLPVLLGAAWMQRRWLKRNPGWHEASPGVVFSRKVTRREAAALAAGGGLAVLDLTAET
                     NAPAVFREQAHYHNLPLLDLVPLKPEHIQAALDHIRAQRAAGRRVLVHCQLGLQRSAM
                     IAAHWLVDCGAAPDLEAAKGMIRKVEPQVVI"
     gene            complement(1496..1723)
                     /locus_tag="PLP58_17270"
     CDS             complement(1496..1723)
                     /locus_tag="PLP58_17270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ10333.1"
                     /translation="MTATHTPDRLELSNGATLIRLSSRRDRLEWQGQPISDIEHRERG
                     GGFVALVNGYRACDQRHVIARLLSKFFDGTL"
     gene            complement(1929..2177)
                     /locus_tag="PLP58_17275"
     CDS             complement(1929..2177)
                     /locus_tag="PLP58_17275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ10334.1"
                     /translation="MKPPLALTLLALLLLLPCCSAWQKQTGLTPGQTLILAAKTQAEY
                     ERLRDLNETSAKSVQDVLPGTDGTPPGAQPWWFGLLGL"
     gene            complement(2268..2585)
                     /locus_tag="PLP58_17280"
     CDS             complement(2268..2585)
                     /locus_tag="PLP58_17280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ10335.1"
                     /translation="MSASDLHQIAQHDTPSSVNVPATWAGLVMWAVGRFGGGIVIAGA
                     FAYAAHRVYEDNAALSRQMLTVLQDLAVVQSKSAAANEQLARAVEALTREAQVGHREL
                     PRR"
     gene            complement(2628..4574)
                     /locus_tag="PLP58_17285"
     CDS             complement(2628..4574)
                     /locus_tag="PLP58_17285"
                     /inference="COORDINATES: protein motif:HMM:NF024199.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ10336.1"
                     /translation="MPGNITFNETVLNVTFNEAGGQCTHEVTDLNVSFSTGIGDSAGT
                     LKAKLESLEGDDRLDATAIKNLPSGPGGDVEWGDIEGTLADQTDLQTALNAKAASSHT
                     HTLADITDAGDAAALDVGTTAGTVCAGDDARLTDARAPTAHASSHVTGGADKIRDATA
                     SQDGLMTAAAMSKLNGIETAADVTDAGNVGAAIHGAAEKTTPVDGDKLGLIDSAASNV
                     LKWLSWSNLKATLKSYLDTLYAAIGHTHAQLHDRSHAVSSTSDHTAGNWKIFHSNGSG
                     QVIELPLGADGTFLKSNGVAAAPTFSTPSGSGDVLGPDEATANGVVLFDGTTGKLVKD
                     GGLLGDSAFLDSSSFAAAEHTHDLADITDAGNSAGLNVGTTAGTVCAGDDARLSDARA
                     PTAHASSHVTGGADKIRDATASQDGIMTAAAMTKLDGIEAAADVTDAGNVGAAIHGAA
                     EKTTPADADKVPLIDSAASNVLKWLSWSNLKAALKSYFDTLYAAIGAGGTVIYSKALS
                     AGVDVTDNAAFQVVSGLGVPVAANKTYVIKWHLSVVTSATGTGSHWGFDGPASPARVM
                     AHAAVQNVSGTAPATGVVHAYGEIAANSAGRTTSQPVILEARVSNGSNAGTVNLTCKV
                     ETAVSGTVTTEGGSWVEYYESTTP"
     gene            complement(4577..4912)
                     /locus_tag="PLP58_17290"
     CDS             complement(4577..4912)
                     /locus_tag="PLP58_17290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ10337.1"
                     /translation="MTLVRGATLRLDMTLTDSAGDPRDLTGASAECAFRRQSAAGEEI
                     GRLTSGDEGGITLDSETGRIIAIAQTADWLPGKIVFDLHLTESNGNILVLVLGELELL
                     ASATVPADE"
     gene            complement(4977..5966)
                     /locus_tag="PLP58_17295"
     CDS             complement(4977..5966)
                     /locus_tag="PLP58_17295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ10338.1"
                     /translation="MRLTLDLNTDQLILGRGNASPVSQVIAARGAGPFIRVQPVRDGQ
                     NAALPEGFEMRWMVKAEGDWSGDSVAYADTFALASGQSFYEGVINYETAELNDLLLVG
                     ESGEKDSVDLTAQLAWRVDDEATWRPSQPVKLTLHNSVWRGTPGAPTPGETALDWLED
                     TLVAGAGISITPSGETLVVAGDRIVKFKAADQLMEDYGPDLIDDDTLQFPAPANSRWR
                     VEMGVLVKATQGEFHAQITAPSGSTLYGRWKGFESVSGTLHPLDVEPLVGTESKVLDN
                     AGATTLEHLLVQHFIVNVAGTGGDVKLRWHGGDYYTNDTWTQAGSWLIAERIA"
     gene            complement(5982..6830)
                     /locus_tag="PLP58_17300"
     CDS             complement(5982..6830)
                     /locus_tag="PLP58_17300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ10339.1"
                     /translation="MPDLHAHPKPRVDVPRDGGRILTRRGLALALARLQAGMRPRLQG
                     MGLPAAQAGSLGFPGPGADPAGNATVDLAAKKILTTIPGRGRLCTRAAFAAALRQVLG
                     GLGFELSITGAALAESAADGGISLRLPRRKENVRIFIQITITDTGAEESPIEREVELA
                     TTLFNSGELAGSIANTSEWEFTQMLELPGGEGDPPDPPPRTKSISVHFWARHQHPSIP
                     GTPGLDDLPFLRPVTLHLKKYDPISSDTLAEYTVNITAAEGEVRNYSVTEEESGAELL
                     ATAVED"
     gene            complement(6835..7032)
                     /locus_tag="PLP58_17305"
     CDS             complement(6835..7032)
                     /locus_tag="PLP58_17305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ10340.1"
                     /translation="MTTTLQPGRRVRLVMPRGGRLMPREAAARLLIDVARQMRMSLQV
                     INSPLSADTQDGAVTGNLKNR"
     gene            complement(7029..7358)
                     /locus_tag="PLP58_17310"
     CDS             complement(7029..7358)
                     /locus_tag="PLP58_17310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ10341.1"
                     /translation="MKITRKQLLESMTGVAREAARPVSRRLRVRAEIEAERPVRVRLI
                     CRAAPTHRERVLALFRDLRHFRAVFQAAFPVNAFVVYGPRKNVTLLDDGSVEVRLTLG
                     LIELTAA"
     gene            complement(7361..8470)
                     /locus_tag="PLP58_17315"
     CDS             complement(7361..8470)
                     /locus_tag="PLP58_17315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ10342.1"
                     /translation="MSVPPVILFGQRNFLLHDPLPEILRARLDVDTLNESWQVGSEDY
                     FIEGAPHPERPGMIIDQIRLREEIPGYAYVAAITALGDSRGTRPTKLLRRSDKRTLDI
                     GWDEFSEELLTWEARWKNVLCTASTDIFTTGIPHAFRDGDAVMFRDLTGGAGITGGSA
                     NALGATYYVRYLTAQSFEVAATPGGSKVNVTSNLTGGTVSLARFVKGARHPDHAHMWL
                     VEISATDDATDWRRVVCQYRGLMESKPYKRIITCNNQQMSSSEPIFWDFEDGWEDARY
                     SQVNLPKIVCTDTYLTTDAPATDEIPWSQGEGATPPDAPDIRSLVITGDVDQITWHWP
                     AGWSRISENTLDSIPLVSVNLKQRVTEFVWPVTLR"
     gene            complement(8467..9312)
                     /locus_tag="PLP58_17320"
     CDS             complement(8467..9312)
                     /locus_tag="PLP58_17320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ10343.1"
                     /translation="MERLRGQLAAERLADYLAILPPSLRALEIARQIHDLEQQAGQAG
                     PETEEMRLEREREILRLQQQQRAARQESAAEVERENAKVEIEKMRLENFRAELAIAEA
                     RQRGEDEVADVMERQLRILELKRRLMEEMGLSEQEALDLATRRIDAEKQVNAEKEKGR
                     GRYDEDGRRADGRRQIKGYSFEKQGGADEARARAGKRVDDARTRIQADYDRAFPGISG
                     APEFGPGRLRDEFQFPGLDAGFGEVPTRAASNAAASETRSSAANDSAGLLKEIAGILQ
                     QGLLE"
BASE COUNT         1487 a         2881 c         3299 g         1740 t
ORIGIN      
        1 cccgcccgcc actcgcgggt tgcacttttg cctttcgggc tgacaaagcg cgatgcacac
       61 gaccgagccc ctccgacccg cgcgcgaacc caggctgctc tggccggcgc tctggcggct
      121 ggcgctggcc ggggcggtgt ttgtgggcgt ctatggcggc tgcaaccact tcaccgccac
      181 gcgcggggat gtgccggtga tgatgttcga gtgggagcgg aacatgcctt tcatcaagga
      241 gatggtggtg ccctactggt cgctggatgt gtttttctgc ggcgcgttct tcctctgccg
      301 cagccggctg gagctgaacc tgctgacgcg caggctcgtg gccgtgactc tgggcagcgg
      361 cgcgtttttc ctgctgttcc cgctgcagct gggcctgccg cgcccggagc cggagggctg
      421 gacggcgccg ctctttcacg cgctctacgc caatgacatg ccctacaacc tcgcgccctc
      481 gctgcacatc agcctgcgct cgctggtgtg ggtgttttac ggcgcgcatc tgcgcggccg
      541 cgcgcgggtg gcggtgaaga tctggttcat cctcatcggc gcgtccacgc tgctggtgtg
      601 gcagcatcac ttggtggatg tggcgggcgg gttcgtcatg ggctgggccg tggccgcgct
      661 gatcccggac ccgcgccagc cgggcacgcg gaacccctcg ctgaaacacg cgcggcgtta
      721 cggcacgggc gcgctggcgt gtgccgggct ggcttttgtc tcggtcgtct tcgtctggcc
      781 tgccatggcc tgcggcatca tggcgctggc ctatgccacg ggactgtccc gcctgctggg
      841 gaaggaaaac ggcacgctct caccctcggc ggagtggtgc ctgctgccgg tgctgctggg
      901 cgcggcgtgg atgcagcggc gctggctgaa aagaaacccc ggctggcatg aggcctcgcc
      961 aggggtggtc ttcagccgca aggtcacaag gagggaggcc gccgccctgg ccgccggcgg
     1021 cgggctggcc gtgctcgacc tcaccgccga gacgaacgcg cccgccgtct ttcgcgagca
     1081 ggcgcactac cacaacctgc cgctgctgga tctggtgccg ctgaagccgg agcacatcca
     1141 ggccgcgctc gaccacatcc gcgcgcagcg cgccgccggc cggcgggtgc tggtccactg
     1201 ccagctcggc ctgcagcgct ccgccatgat cgccgcgcac tggctggtgg actgcggcgc
     1261 ggcgccggac cttgaggcgg cgaaaggcat gatccggaaa gtggagcctc aagtggtgat
     1321 ctgagcggga gccggcacgg gcgcgcgcgc ctctgatgcg cgcggattcc gcggttcgga
     1381 cgctgtgtcc gcctcaccct gtggtggtgg agccaagggg attcgaaggg ttagcctttc
     1441 catgaaaaat caagccttgg aaggaaggca tcgtcttcac accctcttca caccctcaca
     1501 gcgtgccgtc gaaaaatttg ctcagcaggc gggcgatcac atggcgctgg tcgcaggcgc
     1561 ggtagccgtt gaccagggcc acaaatccgc cgccgcgctc gcggtgctcg atgtcgctga
     1621 tgggctggcc ctgccattcg agcctgtcgc ggcgggagga gaggcggatg agggtggcgc
     1681 cgttgctgag ttcgaggcgg tcgggggtgt gggttgctgt catggcttga tcatcgtcta
     1741 acaaacgatg atagctagca caaagagcat tttggatgaa cttttttcag tggaactcaa
     1801 cgattatttt tgcaagcgac cgaccaaggc cccgcccaca agcccctttc accttatccg
     1861 cccccactca gcctgagtgg gggcggccgg tttgcggcca tccttgccgc gcgcaggtag
     1921 ggtagggatc aaaggccgag caagccaaac caccacggct gagcgccggg cggggtgccg
     1981 tctgtgccgg ggagcacgtc ctgaacggat ttggcactag tttcgttgag gtcgcgcagg
     2041 cgctcgtatt cggcctgggt tttggcagca aggatgagag tttggccggg tgtgaggccg
     2101 gtttgttttt gccaggcgga gcaacaaggc aggagcagga ggagggcgag gagagtgagg
     2161 gcaagaggcg gtttcatggc ggacatgcct ggagggcggt gtcaatgagg cgaccgccgc
     2221 tggttcaaac cgcgccaggg gcgcggttga ccagctcagt gtccggctca ccgtctcggc
     2281 agctctctgt gtcccacctg ggcctcgcgg gtgagggcct cgacggcgcg ggcgagctgc
     2341 tcgttggcgg cggcggattt gctctgcacg acggccaggt cctggagcac ggtgagcatc
     2401 tggcgtgaga gggcggcgtt gtcctcatag acgcggtgcg cagcataggc gaaagcgcca
     2461 gcgatgacga tgccgccgcc gaaccttcca actgcccaca tgacgagtcc ggcccaggtg
     2521 gcggggacgt tgacgctgga gggggtgtca tgctgggcga tctggtgcag gtcactggcg
     2581 ctcattgagg tgtgcgggat cggggtttgc gtggcaggaa aaaaaaatca gggggtcgtg
     2641 gactcgtaat actccaccca gctaccaccc tcggtggtga ctgtgccgct gacggcggtc
     2701 tccactttgc aggtgaggtt gacggtgccg gcgttggagc cattggatac acgggcctcc
     2761 agtatgactg gctgggaggt ggtgcgtccg gcggagttgg ctgcgatctc gccgtaggcg
     2821 tggacgacac cggtggcagg agcggtgccg ctgacgttct ggacggcggc atgcgccatg
     2881 acgcgcgcgg gcgaggcggg accatcaaag ccccaatgcg agccggtgcc tgtggcggag
     2941 gtgacgacgg acagatgcca cttgatgaca tacgttttgt tcgcggccac ggggacaccc
     3001 aagccggaga cgacctgaaa ggcggcgttg tccgtgacat ccacgcccgc gctgagagcc
     3061 ttggaataaa tgaccgtgcc gccggcgccg atggcggcat agagggtgtc aaagtaggac
     3121 ttgagcgcgg ctttgaggtt actccaggag agccacttga ggacgttcga cgcggcgctg
     3181 tcaatgagcg gcaccttgtc ggcatcggcg ggcgtggttt tctccgccgc gccgtggatg
     3241 gccgcgccca cgttgcccgc gtcggtgaca tcggcggcgg cctcgatgcc gtccagcttg
     3301 gtcatggcgg cggcggtcat gatgccgtcc tggctggcgg tggcatcgcg gattttgtcc
     3361 gcgccgccgg tgacgtgact ggaggcatgg gcggtgggag cgcgcgcgtc ggagagccgc
     3421 gcgtcatcgc ccgcgcagac ggtgccggcg gtggtgccca cgttgagtcc ggcggagttg
     3481 ccggcgtcgg tgatgtcggc cagatcgtgt gtgtgctcgg cagcggcgaa gctggaggag
     3541 tccagaaagg cgctgtcccc cagcaagccg ccgtctttaa ccagcttgcc cgtcgtgccg
     3601 tcaaaaagca ccacgccgtt cgcggtggcc tcgtctggcc ccagcacatc gccgctgccg
     3661 ctgggggtgg agaaggtggg cgcggcggcc acgccgttgg atttgaggaa ggtgccatcc
     3721 gcgccgagcg gcagctcgat gacctggccg ctgccgttgg agtgaaaaat cttccagttc
     3781 ccggcggtgt ggtcgctcgt cgagcttacg gcatggctgc ggtcatggag ctgcgcgtga
     3841 gtgtggccga tggcggcata gagggtgtcg aggtaacttt tgagcgtggc cttgaggttg
     3901 ctccaggaga gccacttgag gacgttggag gcggcactgt caatgagtcc gagcttgtcg
     3961 ccgtccacag gagttgtttt ctccgccgcg ccgtggatgg ccgcgcccac attacccgcg
     4021 tcggtgacat cggcggcggt ctcgatgccg ttcagcttgc tcatggcggc ggcggtcatg
     4081 aggccgtcct ggctggcggt ggcatcacga attttgtccg cgccgccggt gacgtggctg
     4141 gaggcatggg cggtgggagc gcgcgcgtcg gtgaggcgag cgtcatcgcc cgcgcagacg
     4201 gtgccggcgg tggtgccgac atcgagcgcg gcggcatcgc ccgcgtcggt gatgtcggcc
     4261 agggtgtgcg tgtggctgct ggcggccttg gcattgagcg cggtttgcag gtcggtttgg
     4321 tcggctagcg tgccctcgat gtctccccac tcaacgtctc cgccggggcc cgatggcagg
     4381 tttttaatgg cggtggcgtc gagccggtcg tcaccctcca ggctctcgag cttggctttg
     4441 agggtgccgg cactgtcgcc aatgccggtg ctgaaactca cattgaggtc ggtgacctca
     4501 tgcgtgcatt gcccgccggc ctcgttaaag gtaacattga ggaccgtctc attgaatgta
     4561 atgttgcccg gcatggttat tcgtcggcgg gaacggtggc ggatgctagc aactccagct
     4621 cgccgaggac gaggacgagg atgttgccgt ttgactcggt gaggtggagg tcaaaaacga
     4681 tcttgcctgg cagccagtca gcggtctgcg cgatggcgat gatgcgcccg gtctcggagt
     4741 cgagtgtgat gccgccctcg tctccgctgg tgagtctgcc aatctcctcg cccgcggcgg
     4801 actggcggcg gaatgcgcac tcggcgctgg ccccggtgag gtctcgcgga tcgccggcgg
     4861 agtcggtgag ggtcatgtcc agccgcagcg tggcaccgcg cactagcgtc attttccggc
     4921 gagccgttgg aaaggtctca ggtatgcagc aggtcatggg ggcggaagtg ggttactcaa
     4981 gcgatgcgct cggcgatgag ccaggagccg gcctgggtcc aggtgtcgtt ggtgtaataa
     5041 tcgccgccgt gccagcgcag cttcacatcg ccgccggtgc cggccacatt gacgataaaa
     5101 tgctggacga gcaaatgctc cagcgtcgtc gcgcccgcat tgtcgagcac cttgctctcc
     5161 gtgccaacaa gcggctcgac atccagagga tgcagcgtgc cggacacaga ctcaaacccc
     5221 ttccaccgac cataaagcgt ggatccggaa ggagctgtga tttgagcatg aaactcgcct
     5281 tgagtggctt tgacgagcac tcccatctcc acacgccacc tgctgttggc cggcgctggg
     5341 aattgcagag tgtcatcgtc aatgaggtcg ggaccgtagt cctccatgag ttgatccgcc
     5401 gccttgaatt ttacgatccg gtcgcccgcc accaccagcg tctcgccgga aggcgtgatg
     5461 ctgatgccag caccggccac gagagtatcc tcaagccagt caagcgcggt ctcgccgggt
     5521 gtgggcgcgc cgggagtgcc gcgccagacg gagttgtgga gggtgagttt gaccggctgg
     5581 gaaggccgcc atgtggcctc gtcatccacg cgccaggcga gctgcgcggt gaggtccaca
     5641 ctgtctttct cccccgactc gcctaccaaa agcagatcgt tgagctcggc ggtttcgtag
     5701 ttgatgacgc cctcgtaaaa ggactggccg gaggcgagcg caaaggtgtc cgcataagcc
     5761 acggagtcac cgctccagtc tccctcggcc ttgaccatcc agcgcatttc gaagccttcg
     5821 ggcagggcgg cattctggcc atcgcgcact ggctggacgc ggatgaaagg gccggcgccg
     5881 cgcgcggcga tgacctgtga gacgggcgag gcattgccgc ggccgagaat gagctggtcg
     5941 gtgttgaggt cgagtgtgag gcgcacacac acgcgcgcgt gtcaatcctc cacggcggtg
     6001 gccagcagct ccgcgccgct ctcctcctcg gtcacggaat agttgcgcac ctctccctcg
     6061 gcagccgtga tgttcacggt gtattccgcg agggtgtccg aggagatcgg atcgtatttt
     6121 ttcaggtgca gggtcaccgg tcgcaaaaac ggcaggtcat ccaagccggg cgtgccgggg
     6181 atggagggat gctggtggcg cgcccaaaaa tgcaccgaga tgcttttcgt gcgcggaggc
     6241 gggtccggcg gatccccctc tccgcccggc agttcgagca tttgggtgaa ctcccattcg
     6301 gaggtgttgg cgatgctgcc ggccagctcg ccggagttga aaagggtggt cgccagctcc
     6361 acttcacgct cgatggggct ttcctccgcg cccgtgtcag tgatggtgat ctggatgaaa
     6421 atgcgcacgt tttcctttcg gcgcgggagc cgtagggaga tcccgccatc cgctgcggac
     6481 tcggcgagtg ccgcgccggt gatgctcagc tcaaaaccca gcccgccgag cacctggcgc
     6541 aacgcggctg cgaacgccgc gcgcgtgcaa agccgcccgc ggccggggat ggtggtgagg
     6601 attttttttg ccgccaggtc caccgtggca ttgccggcgg gatcggcgcc ggggccggga
     6661 aaacccaggc tgccagcctg ggcggccggc agccccatgc cctgcaaacg cggcctcatg
     6721 ccagcctgaa gccgcgccag agcgagtgcc agcccgcgcc gggtgagaat gcgcccgcca
     6781 tcgcgcggaa catccacgcg cggcttgggg tgggcgtgca gatcgggcat ggtctcaccg
     6841 attctttaga ttaccagtca ccgcgccgtc ctgagtgtcg gcggagaggg gggagttgat
     6901 gacttggagg ctcatgcgca tctgacgggc cacgtcaatg agcagccgcg cggccgcctc
     6961 gcgcggcatg aggcggccgc cgcgcggcat gacgaggcgg acacggcggc caggctggag
     7021 ggtggtggtc atgcggcggt cagctcgatg aggcccaggg tcaagcgcac ctcgacagat
     7081 ccatcatcca ggagtgtgac gtttttgcgc ggcccataaa cgacaaacgc attgacggga
     7141 aaggccgcct gaaacacggc gcggaaatgc cggaggtccc ggaacaaggc gagcacccgc
     7201 tcacgatgcg tcggcgcggc gcggcaaatg agccgcacgc gcacagggcg ctcggcctcg
     7261 atctccgcgc gcacgcgcag cctgcggctt accggtcggg ccgcctcgcg cgccacgccg
     7321 gtcatggact caagaagttg ttttcgagta attttcatat tcagcgcaag gttacaggcc
     7381 aaacaaattc tgtgacgcgc tgcttgaggt tcacggagac gagcgggatg ctgtcgagcg
     7441 tgttttcgga gatgcggctc cagccggcgg gccagtgcca ggtgatctgg tccacgtcac
     7501 cggtgatgac gaggctgcga atgtcggggg catcgggcgg ggtggcaccc tcgccctgac
     7561 tccaggggat ttcatcggtg gcgggagcgt cggtggtgag ataggtgtcg gtgcagacga
     7621 tttttgggag gttgacttga gagtagcggg catcctccca gccgtcctcg aagtcccaaa
     7681 agatcggctc gctgctgctc atctgctggt tgttgcaggt gatgatccgc ttgtagggct
     7741 tggactccat gagtccgcga tactggcaga cgacgcgccg ccagtccgtg gcgtcatcgg
     7801 tggcggagat ctccacgagc cacatgtggg catggtccgg gtggcgcgcg cctttgacga
     7861 aacgagccag gctgacggtg ccgccagtga ggttggaggt gacgttgacc ttgcttccgc
     7921 cgggtgtggc ggcgacctcg aaactctgcg cggtgaggta acgcacataa taggtggcac
     7981 cgagcgcatt ggcgctgcca ccggtgatgc ccgcgccgcc ggtgaggtcg cgaaacatga
     8041 ccgcatcgcc atcccggaac gcatgaggga tgccggtggt aaagatgtcc gtggaggcgg
     8101 tgcagagcac gtttttccag cgcgcctccc aggtgagcag ctcctccgaa aactcgtccc
     8161 atccgatgtc gagagtgcgc ttgtcggagc ggcgcaaaag tttggtgggc cgcgtgccac
     8221 gcgagtcgcc cagagcggtg atggcggcca cataggcgta acctgggatc tcctcgcgca
     8281 agcggatttg atcaatgatc atgccgggcc gctcagggtg cggagcgccc tcgatgaaat
     8341 aatcctcact gcccacctgc cacgactcat tgagcgtgtc cacatcaagc cgcgcgcgca
     8401 ggatctccgg caacggatca tgcagcagga agttacgctg accaaagagg atgacagggg
     8461 ggacactcat tcaagaagac cttgttgcag gatgccggcg atttctttga gcaacccggc
     8521 actgtcattc gccgcggacg accgcgtctc gctggcggcg gcattgctgg cggcgcgggt
     8581 cggcacctcg ccgaagccgg cgtcgagacc gggaaactga aactcatccc gcaaccgacc
     8641 gggaccaaac tcgggagcgc cagagatgcc ggggaaggcg cggtcatagt ccgcctgaat
     8701 gcgggtgcgc gcatcgtcca cccgcttgcc agcgcgagcg cgagcctcgt cggcaccgcc
     8761 ttgtttttcg aaggagtaac ccttgatttg tcgacgacca tccgcgcgcc gcccgtcttc
     8821 gtcgtaacgt ccgcggccct tttccttctc ggcattgacc tgtttttcgg cgtcaatgcg
     8881 ccgcgtggcg aggtccaatg cctcctgctc actgaggccc atttcctcca tgagccggcg
     8941 cttgagttcg aggatgcgga gctgccgttc catgacatcg gcaacctcat cctctccgcg
     9001 ctggcgagcc tcggcgatgg cgagttcggc ccggaagttt tcgagccgca ttttttcaat
     9061 ttcgaccttg gcgttttcgc gctccacctc ggcggcggac tcctgccgcg cggcgcgctg
     9121 ctgctgctgc aggcgcagga tctcgcgctc ccgctccagc cgcatttcct cggtctccgg
     9181 cccggcctgc ccggcctgct gctccaggtc gtggatctgg cgggcgatct ccaacgctcg
     9241 cagactcggc ggcaggatgg ccaggtaatc ggccaggcgc tccgccgcga gctgcccccg
     9301 caggcgctcc acccgcaccg cctcggcggc ggcggctttt tcagctttgg cggcatcggc
     9361 cggcctggct gccgccccag accagcatcc gcaacgtcgc catcatc
//
LOCUS       DAOMYJ010000202         9206 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Prosthecobacter sp. isolate AMR_MDS_5629
            NODE_2512_length_9206_cov_22.861764, whole genome shotgun sequence.
ACCESSION   DAOMYJ010000202 DAOMYJ010000000
VERSION     DAOMYJ010000202.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867850
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prosthecobacter sp. (wastewater metagenome)
  ORGANISM  Prosthecobacter sp.
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Verrucomicrobiaceae; Prosthecobacter.
REFERENCE   1  (bases 1 to 9206)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9206)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.30
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:34:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,742
            CDSs (total)                      :: 4,684
            Genes (coding)                    :: 4,676
            CDSs (with protein)               :: 4,676
            Genes (RNA)                       :: 58
            tRNAs                             :: 55
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9206
                     /organism="Prosthecobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2512_length_9206_cov_22.861764"
                     /isolate="AMR_MDS_5629"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1965333"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..241)
                     /locus_tag="PLP58_17325"
     CDS             complement(<1..241)
                     /locus_tag="PLP58_17325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ10344.1"
                     /translation="MNTKLLLLPALLSAALTGCETPSSSMSAQTPASGALTSAASGSS
                     GMAVSNAAEEVPKPAGIGAINYPPPQPPDIPAPPPA"
     gene            434..3454
                     /locus_tag="PLP58_17330"
     CDS             434..3454
                     /locus_tag="PLP58_17330"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional serine/threonine-protein
                     kinase/formylglycine-generating enzyme family protein"
                     /protein_id="HRJ10345.1"
                     /translation="MQGLLAAGAAGVTRELRAWQPPSVADLQKLLPQYEITAFIARGG
                     MGAVYKGVQRSLERTVAIKILPPDITDDGEANYAQRFKQEARAMAKFKHPGIVTVYEA
                     GEVELGRTLDIGRALGPPSAAGGDSRRLEDQPPYPAVEVPLDGGLGTSRPTLLYFVME
                     FIEGTDVAHLVAAQGQLPPQEAIRITTAVCDALAYAHARGVVHRDIKPSNIMIDADGQ
                     VKVADFGLAKITAQDSGGLTRSSVAMGTPDFVAPEAMLAGTLVDQRADIYAVGVMLYQ
                     MLTGHIPRGRFSPISAVVPQSDPKLDAIVDKAMQTDREKRYSSAVEMKTEVESVCRAS
                     VPDATAGKPSHRDGLQSRHRAPLLIAAAVIVLGVGAWMMWKKQTEEGRAAGPPAAANG
                     SAAASEITPFQPPPGPPGLAKTYDLRNIINMALVQGDRRVLILEKDNMRLFDLATEQA
                     VWQRSGVHQSGRVYDLADGQRAIRFGRTNDEFHASVMDLTTGNDLGSWSTANPFKGPS
                     NNTSAVSPDGRHFCVAAQTSPKPGAPSESATHVWVLAADTMKTVVEWHLPSYPQLYTA
                     AWLDAQRFLVSTSRPEALLTFDLRSTAAKPLPHPARLHNFIGTDAAGQFLAGAHLGNL
                     ALVPLAGHQNKPTVLRQPFGPRDISFAGSETLVVLGLDASDLHVYDLPSRAAAAEFHR
                     PNENGTLLVSRDGSFAITKEIQQQGNEKITQLNLWRLPKAGAAKGGAAAPPAAAKGPT
                     GTSAATAALGGASALPSSATKDAHFTNSLGMKFMPVPGTDVLFCIHETRKKDYAAYAA
                     ETPGVDMTWKQPSIYHGLPVGAGEDHPVTQINLADCQGFCAWLSAREGRRYRLPTDLE
                     WSWAVGIGDREDASALPEKRGGGAGNVYAWGTEWPPPPRVANIADESLRQVAAHMPNR
                     LIPTYEDGFATTAPVMSFPPNALGIYDLAGNVWELIPDWMDAEKQDVLRRGSSFNNGS
                     LDPARAYVAASVRNRRNPHARSEDGGFRVVVEKP"
     gene            3494..4246
                     /locus_tag="PLP58_17335"
     CDS             3494..4246
                     /locus_tag="PLP58_17335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ10346.1"
                     /translation="MAAHSPKGDGRFPTTEWTLVERLKHDDVEVSSRALNELCAQYHY
                     PLYCLIRARGLAHHDAEDALHDFLAKLLRLEAFEDLDREKGRLRTFLAKTLERSLISR
                     YHRESRRERREVAAEDLRLPFDPDIADRYARESTDGLSPDKLFDRKWCEQLLGRVLRS
                     LAGDYATRGKGRLFETLRPVLLAGGSLRGHDAAALAGTLGMTEPALRTALVRLLRDYR
                     RLLEHEVRQTVDSKEAVEAEIADLMAALGNDG"
     gene            complement(4357..5733)
                     /locus_tag="PLP58_17340"
     CDS             complement(4357..5733)
                     /locus_tag="PLP58_17340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009958117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1552 domain-containing protein"
                     /protein_id="HRJ10347.1"
                     /translation="MSHLINRRACLRGLGASLGLPLLETMGWADAAKGKTSYKPPVRM
                     GFMYMPHGVIMDQFWPKSPESFRNSPPPALESLRPILDQCLMVKGISGVGIEPFGGAP
                     HALELSTWLTACLPDANKRSQIHIAISADQIFANYYGALTALPSLELATMPQTHKENQ
                     AGLNEGYYSHCSFRSPTQAMPAEINPRSVLNRLFGKTGQEGKTTATDPLDRQMLDLVI
                     GGAKDLRRTLPHGDQQKLDEYLDSVRSVERRIAAIEYRQKEAALEKAGVSSSKRKASD
                     SPPIEVKIPEGDKRSEYMQVMCDLTVLAFQTDTTRVSTYIGSTPNGVSYPEIGVSNTH
                     HSYTHHNNEAEKAGKVAAITKFNIDQFAYMVNKMAALREGDGTLLDNSIMMWGSGLED
                     GNKHTRENLPFIIAGKGGGSINTGRFINTVKGNQGDLLTTLLACAGVPLDRPIGIATR
                     EIKEMMRA"
     gene            complement(5752..7617)
                     /locus_tag="PLP58_17345"
     CDS             complement(5752..7617)
                     /locus_tag="PLP58_17345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009958116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1592 domain-containing protein"
                     /protein_id="HRJ10348.1"
                     /translation="MTFPVKNRALILTLLVLIPAAPGLLRAEQDKELAAMQAEAQKAF
                     KDHVEPFVKQYCVECHGDRPKAGLNLRVAVRKPGDPAFARKWMEAIANVNAHDMPPED
                     ADQPKDDERKRFLDALAQIKYLAARDPGPFVIRRLTKVEYGNTLHDLLGVDASLANEL
                     PDEVPGEGYLNSLSPMQTEQYLGIANAALRQMPDDVQKRLFGGGEVHVRDVARKIART
                     AYRRPPTQNELDVLVSVFDLGTANKLDRSAALKLMLKAVLVSPQFLFITPSKDAPTGE
                     KIMPLDDYQLASRLSYLLWATMPDAELSKLADEGRLRDPDMLEAQTMRLLADNRSRAL
                     FDGFGAQWLGLDKLATQPFDEKKFPQMTPEMRRAMVDEARLLFDSIVRENRSVVSFVS
                     ADYTFLNRTLATIYGLEKTITGSDLQKVSLTDANRGGILGMPGVLAATSFATRTSAVN
                     RGVWVLEQVLGQHVPPAPPNVPALEKQDQKKVANLTLRQRTELHRTNAVCANCHKILD
                     PIGFGLENFDAIGRWRDKDESGGPIDAVGELPGGKRFTSPKELKALIAARETDLARNL
                     TEKLLAYALCRQLDGYDHIIVDQMLQTIAKDGFKMQTLISEIVTSYPFLNRRVQE"
     gene            complement(7688..8581)
                     /gene="folD"
                     /locus_tag="PLP58_17350"
     CDS             complement(7688..8581)
                     /gene="folD"
                     /locus_tag="PLP58_17350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007418642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004488 - methylenetetrahydrofolate
                     dehydrogenase (NADP+) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase
                     FolD"
                     /protein_id="HRJ10349.1"
                     /translation="MQLISGTAVAEKVLEECRRDIAALAAAGMKPGLAVVLVGDDPAS
                     RAYVRSKDKKCRDLGLHSVKHELPATTSQAELLALVHELNADPAIHGILVQSPPPPHI
                     DEAAIVRAIDPAKDVDGFHPVNVAKLALEDPAGFVPCTPLGCQRLLMDAGIETRGAEV
                     VVLGRSMIVGKPMSLLLMAKGPGGDATVTVAHSRTRDLAAVTRRADILIAAIGRPEFV
                     RAEHVKEGAVVIDVGINRVPAPETAKGSKLVGDVAFAEVAPKCRAITPVPGGVGPMTI
                     AMLMANTVKACRQLLAGAVLE"
     gene            8819..>9206
                     /locus_tag="PLP58_17355"
     CDS             8819..>9206
                     /locus_tag="PLP58_17355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ10350.1"
                     /translation="MAALPLPAQEAPKNWTDRETRLANEYLSLLVSQPEYGRVVDLLW
                     SLYEKHGATSLLMENVSQQAEQARHPVLRLVQGHLLRRAGNLPGAVALYDEVLAAQPE
                     NVAALRARADAARESGDADAAYACLEK"
BASE COUNT         1612 a         2824 c         3085 g         1685 t
ORIGIN      
        1 gggcgggcgg cggggcgggg atgtcaggcg gctgaggcgg cgggtaattg atcgcaccga
       61 tgcccgccgg tttcggcacc tcttccgcgg cgttggagac ggccatgccc gaagatcccg
      121 atgcggcgct ggtgagcgcg ccggaagctg gcgtttgggc tgacatcgag gaggacggcg
      181 tttcgcagcc agtgagggcg gcgctgagga gggcgggcag gagcaggagc ttggtgttca
      241 tgggcggaga ggcgttcaca actgccaaac cagggcggcg ggagttcgtg acagaaattt
      301 ttcaagattg gcaaaatgcc ttgccgcgcc tgccgtcacc ccgcacaatc actccccgat
      361 ggctgatgct gcggacagtt cacctgagag tgcgccgccg gatggggcga cggaaaagct
      421 gacccctgcg gccatgcagg gcctgctggc cgcaggcgcc gccggggtga cgcgggaact
      481 gcgcgcctgg cagccgccga gcgtggcgga cttgcagaag ctgctgccgc agtatgagat
      541 cacggctttc atcgcgcgcg gcgggatggg cgcggtgtac aaaggggtgc agcgcagcct
      601 ggagcgcacg gtggccatca agatcctgcc gccggacatc acggacgatg gcgaggcgaa
      661 ctatgcgcaa cgtttcaaac aagaggcgcg cgcgatggcg aagttcaagc atcccggcat
      721 cgtcactgtg tacgaggcgg gggaggtgga gctgggtagg acgcttgata taggtagggc
      781 gcttggtcct ccaagcgccg ctgggggtga ttcacggcgg ctggaggacc agccacccta
      841 ccctgccgtt gaggtgccct tggacggcgg cttggggaca agccgcccta cccttcttta
      901 tttcgtcatg gagttcatcg agggcacgga tgtggcccat ctcgtggccg cgcagggcca
      961 gctcccgccg caggaggcca tccgcatcac caccgccgtg tgcgatgcgc tggcctatgc
     1021 gcacgcccgc ggcgtggtgc atcgggacat caagccctcg aacatcatga ttgatgcgga
     1081 cggccaggtg aaggtggcgg actttggcct ggcaaagata accgcgcagg acagcggcgg
     1141 cctcacgagg agcagcgtgg ccatgggcac acccgacttt gtggcaccgg aggcgatgct
     1201 tgcgggcacg ctggtggatc agcgcgcgga catttatgcc gtgggcgtga tgctttacca
     1261 aatgctcacc ggccacatcc cgcgaggccg cttctccccc atcagcgccg tggtgccgca
     1321 gagcgatccc aagctggatg ccatcgtgga caaggcgatg cagaccgacc gcgagaaacg
     1381 ctactccagc gcggtggaga tgaagacgga ggtggagagc gtttgtaggg cgtctgtgcc
     1441 agacgccact gccgggaagc cgtctcacag agacggccta caatcgaggc accgcgctcc
     1501 tctcctcatc gcggcggcag tgatcgtgct gggcgtgggt gcatggatga tgtggaagaa
     1561 gcagacggag gaaggtaggg cggctggtcc gccagccgcc gcgaatggct ctgcggcagc
     1621 aagtgagatc acccccttcc aaccgccgcc cggtccgccg ggtctggcga aaacctatga
     1681 cctgcggaac atcatcaaca tggctttggt gcagggtgac cggcgggtgc tgatccttga
     1741 gaaggacaat atgcgcctgt tcgacctcgc cacagagcag gccgtctggc agcggagcgg
     1801 agtgcatcag tcaggccggg tttacgatct ggcagatggc cagcgagcca ttcggtttgg
     1861 cagaaccaac gacgagttcc atgccagtgt gatggacctc acgacgggga atgacttggg
     1921 ttcgtggagc acagccaatc cattcaaagg tccttccaac aacacgtcag ccgtctcacc
     1981 cgacgggcgg catttctgcg tggcggcgca gacatcgccc aagcctggag cgccatctga
     2041 gtcggccacc catgtatggg tgctggcggc ggacacgatg aagaccgtcg tggagtggca
     2101 ccttccatcg tatccgcagc tctacactgc tgcctggctg gatgcgcaac gctttctggt
     2161 ctccaccagc cgccccgaag ccttgctcac ctttgacctt cgaagcacag ctgccaagcc
     2221 cctgccgcat cctgcgcgtc tgcataattt catcggcacg gacgctgcgg gtcagttcct
     2281 cgctggtgcg catttgggca atctggcgct ggtgccgctc gccgggcatc aaaacaagcc
     2341 cactgtcttg cgccagccat tcggcccgcg cgacatcagc ttcgcaggca gcgaaacgct
     2401 ggtggtgctg gggttggatg cgagcgatct ccacgtctat gacctgccga gccgggcggc
     2461 ggcagccgag ttccaccgtc ccaatgagaa cgggaccttg ctagtgagtc gggatggcag
     2521 ttttgccatc accaaagaaa tccagcagca aggaaacgag aagatcacgc agctcaatct
     2581 gtggcggctg ccgaaggcgg gagccgccaa aggtggggcg gctgctccgc cagccgccgc
     2641 gaagggtccc acaggaacga gtgctgcaac ggcggcgctt ggcggagcaa gcgccctacc
     2701 ttcctccgcc acgaaagacg cccatttcac caacagcctc ggcatgaagt tcatgcccgt
     2761 accgggcacc gacgtgctct tttgcatcca cgagacgcgg aagaaggact acgcggcgta
     2821 tgcggcggag acgccagggg tggacatgac gtggaagcag ccatccattt accacggcct
     2881 gcctgtcggt gccggggagg atcatccggt gactcaaatc aatctggcgg actgccaggg
     2941 cttctgcgcc tggctgagcg ccagggaagg tcggcgttac cgtctgccca ccgacctcga
     3001 gtggagctgg gcggtgggca tcggtgatcg cgaggacgcc agcgcactgc cggaaaagcg
     3061 gggtggtgga gctggcaacg tttacgcctg gggcacggag tggccgccgc cgccgcgcgt
     3121 ggcgaacatc gctgacgaat ccctgcgcca ggtggccgcg cacatgccga accgcctcat
     3181 ccccacttac gaggacggtt ttgccaccac cgcgccggtg atgagctttc cgcccaatgc
     3241 actgggcatt tacgatctgg cggggaacgt gtgggaactc atcccggact ggatggacgc
     3301 ggaaaagcag gatgtgctgc ggcgtggaag cagcttcaac aacggcagcc tcgatcctgc
     3361 ccgcgcctat gtggccgcca gcgtgcgcaa ccgccgcaac ccccatgcgc gcagcgagga
     3421 cggcggcttc cgcgtcgtgg tggagaagcc ttgacgatga ccaaggcagc atttccaatc
     3481 gacgcacgag cacatggcag cacactcccc caaaggcgac ggcagattcc ccaccacgga
     3541 gtggacgctg gtggaacgac tgaagcacga cgatgtggag gtcagcagtc gcgcgctcaa
     3601 tgagctgtgc gcgcagtatc actacccgct ctactgcctg atccgcgcgc gcgggctggc
     3661 gcaccacgac gcggaggacg cgctgcatga cttcctggcc aagctgctgc gcctggaggc
     3721 tttcgaggac ctggacaggg agaagggccg gctgcgcacc tttctggcga agacgctgga
     3781 acgctcgctc atctcccgct accaccgtga aagccgtcgc gagcggcggg aggtcgcggc
     3841 ggaggatttg cggctgccct ttgacccgga catcgccgac cgctacgcca gggagtccac
     3901 ggacggtctg tcgcctgaca agctcttcga ccgcaaatgg tgcgagcaac tgctgggccg
     3961 tgtgctgcgc agcctcgcgg gagactatgc cacgcgtggc aaggggcgtc tttttgaaac
     4021 gctgcgtccc gtcctgctgg cgggcggcag cctgcgcgga cacgatgcgg ccgcgctggc
     4081 cggcacgctg ggcatgacag aacccgcgct gcgcaccgcc ctggtgcgcc tgctgcggga
     4141 ctaccgtcgg ctgctggagc atgaggtgag acagacggtg gactcgaaag aggccgtgga
     4201 agccgaaatc gcggacctga tggccgcgct gggcaacgat ggatgacacg gatgcgtgcg
     4261 gtgccaccac cgtcgtggga ggcgcacctt ggcccgtcat cggctccgac cggtcgtcag
     4321 gggccaccat gctgctgaca taccggcagc gtcaactcac gccctcatca tctccttgat
     4381 ctccctcgtc gcgatgccga tcgggcgatc caggggcact ccggcgcagg cgagcaacgt
     4441 ggtaaggaga tcgccttgat tgcctttcac ggtgttgatg aagcggccgg tgttgattga
     4501 gccgccgcct ttgccagcaa tgatgaaggg caggttctcg cgggtgtgct tgttgccgtc
     4561 ctccaggccg ctgccccaca tcatgatgct gttgtcgagc agcgtgccgt cgccttcgcg
     4621 gagggctgcc attttgttca ccatgtaggc gaactgatca atgttgaact ttgtgatcgc
     4681 ggccactttg cccgcctttt ccgcctcgtt gttgtgatgc gtgtaggaat ggtgcgtgtt
     4741 agacacgccg atttcgggat acgagacgcc attcggcgtg gagccgatgt aggtgctcac
     4801 gcgcgtggtg tccgtctgga aggcgagcac ggtgagatcg cacatgacct gcatgtattc
     4861 gctgcgcttg tcgccctcgg gaattttcac ctcgatgggc ggcgagtcac tggccttgcg
     4921 cttgctgctg ctgacaccgg ccttctccag cgcggcctct ttttgccgat actcgatggc
     4981 ggcgatgcgg cgctcgacgg agcgcacgct gtcgaggtat tcatcgagct tctgttgatc
     5041 gccatgcggc aaggtgcgcc gcaggtcctt cgcgccgccg atgacaaggt cgagcatctg
     5101 gcggtcgagc gggtcggtgg cggtcgtttt gccctcctgg cccgttttgc caaacaaacg
     5161 gttcaaaaca ctgcgcggat tgatctccgc cggcatcgcc tgcgtgggcg aacggaagct
     5221 gcaatgcgag tagtagcctt cattcagccc cgcctggttc tccttgtgtg tctgcggcat
     5281 cgtcgccagc tccagcgaag gcagcgcggt gagcgcgcca tagtaattgg cgaaaatctg
     5341 atccgccgag atcgcgatgt ggatctggct gcgcttgttt gcatccggca aacaggctgt
     5401 gagccatgtc gaaagctcca gcgcatgcgg cgcgccgcca aacggctcga tccccacgcc
     5461 cgagatgccc ttcaccatca ggcactggtc caaaatcggc cgcagcgact ccagcgcggg
     5521 cggtggcgag ttgcggaagc tctccggcga ctttggccag aactgatcca tgatcacccc
     5581 atgtggcatg tacatgaacc ccattcgtac cggcggcttg tagctcgtct tgcctttcgc
     5641 ggcatccgcc cagcccatgg tttcaagcaa aggaagcccc agcgaggctc caaggccccg
     5701 gaggcaggcg cggcgattga tgaggtggga catggcagtg gtgttgcggg gttattcctg
     5761 aacgcgtcga tttaaaaacg gataactcgt cacgatctcg ctgatgagcg tctgcatttt
     5821 gaagccgtcc ttcgcgatgg tttggagcat ctggtccacg atgatgtggt cgtagccgtc
     5881 gagctggcgg cagagggcgt aagcgaggag tttttcggtg agattgcggg cgagatcggt
     5941 ctcgcgggcg gcgatgaggg ctttgagttc cttcggtgag gtgaagcgct tgccgccggg
     6001 gagttcgccg acggcatcaa tcgggccgcc ggactcgtct ttgtcacgcc agcggccgat
     6061 ggcatcgaag ttctccaaac cgaagccgat gggatcgagg atcttgtggc agttggcgca
     6121 gacggcgttc gtgcgatgca gctcggtgcg ctggcgcagg gtgagattgg cgactttttt
     6181 ctgatcctgc ttttcgagcg cgggcacgtt cggcggcgcg ggcgggacgt gctggccgag
     6241 cacctgctcc agcacccaga ctccacggtt caccgcactg gtgcgcgtgg cgaaggaggt
     6301 ggcggcgagc acgccgggca tgccgaggat gccgccgcgg ttggcatcgg tgagcgacac
     6361 cttttgcagg tcgctgccgg tgattgtctt ttcgaggccg taaatcgtcg cgagcgtgcg
     6421 gttgaggaag gtgtagtcgg cgctgacgaa gctgacgacg ctgcgattct cgcgcacgat
     6481 gctgtcaaag agcagcctcg cctcgtcaac catggcgcgg cgcatctccg gcgtcatttg
     6541 cggaaatttt ttctcgtcaa agggctgggt ggccagtttg tccaggccga gccactgagc
     6601 gccgaagccg tcaaacaacg cccgcgagcg attgtcggcg agcaggcgca tcgtctgcgc
     6661 ttcgagcatg tccgggtcgc gcagcctgcc ttcgtcggcg agcttcgaca gctccgcgtc
     6721 gggcatcgtg gcccacagga gatacgagag acgcgaggcg agttggtaat cgtcgagcgg
     6781 catgatcttc tcgcccgttg gcgcgtcttt ggacggtgtg atgaacaaaa actgcggcga
     6841 gacgagcacg gccttcagca tgagtttcag cgcggcgctg cggtcgagct tgttggccgt
     6901 gccgaggtcg aaaacactga ctaacacatc aagctcgttt tgagtgggtg gtcggcggta
     6961 agcggttcgg gcgatttttc gtgcgacatc tctgacgtga acctccccgc ccccaaaaag
     7021 cctcttctgc acatcatcgg gcatttgtcg cagcgcggcg ttcgcgatgc cgagatactg
     7081 ctcggtctgc atgggcgaga gggagttgag gtagccttcc ccgggcactt catcgggcaa
     7141 ttcgttcgcg aggcttgcat cgacaccgag caaatcgtga agagtgttgc catactcgac
     7201 tttcgtgagg cggcggatga caaaaggtcc cggatcgcgt gccgcgaggt atttgatctg
     7261 cgcgagcgca tcgaggaaac gcttccgctc gtcgtccttc ggctggtcgg catcctccgg
     7321 cggcatgtcg tgcgcgttga cgttggcaat ggcttccatc cacttccgcg caaaagccgg
     7381 atcacccggc ttgcgcacgg cgacgcgcag attcagcccc gccttggggc ggtcgccgtg
     7441 gcactcgacg cagtattgct tcacaaacgg ctcgacgtga tctttgaagg ctttctgcgc
     7501 ttccgcctgc atggcggcca gttccttgtc ctgctccgcc cgcagcaaac ccggcgcggc
     7561 ggggatgagg acaagcaggg tcagtatgag ggcgcggttt tttacaggga aagtcattcg
     7621 gcgaacaagg gggcggcctt tatcacggcg catccacgac cgctctatcc tgcggcgccg
     7681 gaaggtttca ctccaacacg gcgcccgcca gcaactgccg gcacgccttc actgtgttcg
     7741 ccatgagcat ggcgatggtc atcgggccga ctcctccggg caccggggtg atggcgcggc
     7801 acttgggagc cacctcggca aaggcaacat cgccgacgag cttgctgcct tttgccgttt
     7861 cgggcgcggg cacgcggttg atgcccacgt caatgaccac cgcgccctcc ttcacatgct
     7921 ccgcgcgcac aaactccggc ctgccgatcg ccgcaatgag gatgtccgcc cggcgcgtca
     7981 ctgcggcgag atcccgggtg cgcgaatgcg ccaccgtgac cgtggcgtca ccgcccggac
     8041 ctttcgccat caaaagcagc gacatcggct tgcccacgat catgctgcgg ccgagcacca
     8101 ccacctccgc gccgcgcgtc tcgatgccgg catccatcag caggcgctgg cagcccaggg
     8161 gggtgcatgg cacaaaaccg gcggggtcct ccagcgccag cttggccaca ttcaccgggt
     8221 ggaatccgtc cacatccttc gcggggtcta tcgcgcgcac gatggccgcc tcgtcaatgt
     8281 gcggaggcgg cgggctctgc accaggatgc cgtggatggc ggggtcggcg ttcagctcat
     8341 ggacgagcgc cagaagctcc gcctgggagg tcgtggcagg cagctcgtgc ttcacggagt
     8401 gcaggccgag gtcgcggcat tttttgtcct tcgagcgcac ataagcgcgg gaggcggggt
     8461 cgtcccccac gagcaccacg gccaggccgg gcttcatccc cgccgccgcc agcgcggcga
     8521 tgtcacggcg gcattcttcg aggacttttt cagcgacggc ggttccggag atcaattgca
     8581 tgcccttctt ttgaagcagg gaaaagctcg cgtcaatgaa gcggggaccc gatgcctggt
     8641 aaacaaaggt gcccgcgctc aaagtctcct gccccgcatt gtggttttca agatggcctg
     8701 tgctatgctc gtttgtcctg cgatgccgct tccattgtct caaaacccct tcctccgacc
     8761 gcgcccgcgt gctggaatca tgcgtgctgg agttcgggct ttagccgccc tggttgcgat
     8821 ggcggcgctg ccgctgcccg cgcaggaagc gccgaaaaat tggacggacc gcgagacacg
     8881 gctggcgaat gaatacctct ccctcctggt cagccagccg gagtacggcc gcgtggtgga
     8941 tctgctctgg agtctttatg agaagcacgg cgccaccagc ctgctcatgg aaaatgtctc
     9001 ccagcaggcg gagcaggcgc ggcatcccgt gctccgtctg gtgcaggggc acctgctgcg
     9061 gcgcgcgggg aacctgcccg gcgcggtggc gctttatgac gaggtgctgg cagcgcagcc
     9121 ggagaatgtg gcagccctgc gcgcgagggc ggacgcggcg cgcgagtcgg gcgacgcgga
     9181 cgcggcctac gcttgtctgg aaaaac
//
LOCUS       DAOMYJ010000203         9116 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Prosthecobacter sp. isolate AMR_MDS_5629
            NODE_2538_length_9116_cov_19.250745, whole genome shotgun sequence.
ACCESSION   DAOMYJ010000203 DAOMYJ010000000
VERSION     DAOMYJ010000203.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867850
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prosthecobacter sp. (wastewater metagenome)
  ORGANISM  Prosthecobacter sp.
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Verrucomicrobiaceae; Prosthecobacter.
REFERENCE   1  (bases 1 to 9116)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9116)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.30
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:34:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,742
            CDSs (total)                      :: 4,684
            Genes (coding)                    :: 4,676
            CDSs (with protein)               :: 4,676
            Genes (RNA)                       :: 58
            tRNAs                             :: 55
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9116
                     /organism="Prosthecobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2538_length_9116_cov_19.250745"
                     /isolate="AMR_MDS_5629"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1965333"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..350)
                     /locus_tag="PLP58_17360"
     CDS             complement(<1..350)
                     /locus_tag="PLP58_17360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ10351.1"
                     /translation="MSERSLLSKPWLWAHMLSLEAPAVALVWTASLARLHGSKLPPGV
                     LPGLALVVWVIYLLDRVLDTLGQPEAELDERHRFYLRHRLWIAGLAVPAGAAAAVWLA
                     FWRIPAGLLVEALVL"
     gene            452..1567
                     /gene="dprA"
                     /locus_tag="PLP58_17365"
     CDS             452..1567
                     /gene="dprA"
                     /locus_tag="PLP58_17365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009963589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-processing protein DprA"
                     /protein_id="HRJ10352.1"
                     /translation="MTRQEACLALNLIPQIGPVRARKLIAAFGGPEQVLKASARDLTR
                     VEGIGEAHAQAVAGWEAQVDLPGELAKVKARGITLLTETDELFPPSLRGLRDAPLLLY
                     VWGQLLPRDHHAIGIVGSRHATVYGLSATKRLGYQIAFAGRTVISGLARGIDTAAHEA
                     ALAAKGRTIAVIGSGIGKLYPPENQALAGRIAQSGAVVSEYPVDRIADKQTFPYRNRI
                     VAGWSAGMLVVEAPARSGALITAQQAVDNGRTVYAVPGPIDKPTSQGCNRLIQQGAKL
                     VIEAEDILADLDTLFPPAHIPQDARADNAPPAAPPVSLDEEIVLKAVKDGAAHIDEII
                     TASGLTPATVNVCLMRLEMKRLLRALPGRCYEAAGSS"
     gene            1596..3692
                     /locus_tag="PLP58_17370"
     CDS             1596..3692
                     /locus_tag="PLP58_17370"
                     /EC_number="4.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF017076.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_function: GO:0016849 - phosphorus-oxygen lyase activity
                     [Evidence IEA];
                     GO_function: GO:0032555 - purine ribonucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HRJ10353.1"
                     /translation="MNFRLLRLAPFLITLVMGVLLLLLDWLMNEKGGLEFIQRFELAS
                     YDWRMRQMDPPADAGRQAGVLHVDERGTERLKQTLKVNWPVPRWIHGVILDELTAQGA
                     LAVGYDIFFTRMRPEDKAPAMSTAAAPSSDEVFIQALRRSGRTVLGAPMDPSRSGDNV
                     LLPAAPFAQAAATAGHALGKADPDGVLRRVLPLVEDASAGRVWQLGIQTAALALGLDL
                     NRAQLTPWRLTVPDAQGRTHYIPLDGHGRLVIPWRLSATPAASARNRSFFDVLGDSQR
                     RRSGQGGGPSSFWQDRVVIVGSFGANPPLADAGPTPLGARTPYCATHWNIADAMMRGA
                     FIHVPGPATRAGVTLALMLAVSVLSWKLRALWATAAAALLSAAYAACCVWLLHARGLW
                     LPLALPLLGATSITHVTMTFFRLVIEKGHKRRLRSVFQQIVSPRLLEMILDQPHVSWA
                     PHQKMMTVFFADIRGFTALTDDSRLDRIGLTPGQEPPDPAQSQALRDRHALDTVNLYL
                     GLVVDTIKKHGATLDKYMGDCVMAFWGAPLDDRHHALKAVRAAVEVQRRIHDLNERRR
                     MENERIGQENRARMADGRPPLQPHALLTLGTGINTGLMTAGFMGSEAHLSNYTVFGRE
                     VNLAARLESLSGSSRILISQATLDAVSADDPALAASARGIGAHPMKGIQQPVPVYEVS
                     WEETRKNNNEAQAGAL"
     gene            3733..4335
                     /locus_tag="PLP58_17375"
     CDS             3733..4335
                     /locus_tag="PLP58_17375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ10354.1"
                     /translation="MKPKSKKKAANKKAAPRRAIQKRGALKKKTTSAAAKKLTSPAPK
                     PKPAVKAPPPVIKGGIREYFVVRAAKTPAALLSPLTSNGKPRISLNEGEIWIEDSDGV
                     TTPETLPYELESGDTICTYNPGDDPSDPATLPEIQSFEVDCYESDGSYYEDVIVYDYF
                     DDCISVDLTVLDECIEEPEPATDEDPYTCNDGTFSIGIEG"
     gene            4373..4768
                     /locus_tag="PLP58_17380"
     CDS             4373..4768
                     /locus_tag="PLP58_17380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ10355.1"
                     /translation="MPLYLSVLKLTGSVTWRNGTTGTFIPLNNCQSFDTAATATAQFR
                     LRTNDASIEIHQFESPMQSPPCPVPHKGSTMIMVLNRDPANLKKRRGNLTPRSRKALG
                     ASGKRRELKRGSATLQSVQTGGVTLEIRT"
     gene            4876..6327
                     /locus_tag="PLP58_17385"
     CDS             4876..6327
                     /locus_tag="PLP58_17385"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HRJ10356.1"
                     /translation="MKTPLILLTLASAAFAQPPNILLVLADDLGYGDLACYGAKDLRT
                     PHLDRLAAEGLRFTDCYAGHANCSPSRTALMTGRTPTRVGVRNWIPEDSPVHLRRTET
                     TLPTLLRQAGYTTCLSGKWHLNGAFNHPSQPQPGDHGFDHWFATQNNAYPSHRNPANF
                     VRNGTPLGKLEGYAAHLVADEALRWLDARDKSKPFFLYIAFHEPHEPIASDARYTALY
                     PHEDPAYSAHHGNITQMDDAFGRLMAKLDTDGLRESTLVFFTSDNGPAITAQHPYGSA
                     GPLRDKKGSLWEGGIRVPGILRWPGKVQPGATSAEPVSGVDLLPTLCAAAGVTPPQDR
                     ALDGASILPALESRPVTRSTPLYWHFNRASGEPKAALRAGDWKILATLDKAPATRTND
                     ITEQDERDFKEARLQNFMLHNLRADPAEQRDLAAAEPAKLAEMKALLEAKYAEVQAES
                     PTWPAWTFTGAEGKKIEWPDYVRKKKAAPKKQP"
     gene            complement(7178..>9116)
                     /locus_tag="PLP58_17390"
     CDS             complement(7178..>9116)
                     /locus_tag="PLP58_17390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009960205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HRJ10357.1"
                     /translation="AKFINPDHENEELRVKDAADALAGARDIIAERISDNAEARAALR
                     KLFAEQATVLSKVMFGKETEADAQKFRDYFDWTEPLKSIPSHRMLAIRRGEKEGFLLM
                     RIQPPEEAATQLVTHMFAKGGNTCTEQMKLACADSYKRLLGPSMETEFRLESKKKADA
                     EAVKVFADNLRELLMAAPLGQKRLLGIDPGFRTGCKTVVLDAQGQLLFNDVIYLLGEG
                     NSLMHAKTLVLNLVERFKIEAIAIGNGTASRETEAFINKIGVPKQIPVIMVNESGASI
                     YSASEVARDEFPNHDVTVRGAVSIGRRLMDPLAELVKIDPKSIGVGQYQHDVDQKLLK
                     DGLDNIVISCVNNVGVEVNTASKQLLSYVSGLNSTHAANIVAFRNENGPFKSRKDLLK
                     VPRLGDKAFEQAAGFLRIRDAAHPLDSSAVHPERYPLVEQMAADLGCTVADLMARPEL
                     RQKLDLKKYVSADVGLPTLQDIMSELAKPGRDPRKQFEVFKFQEGVNEMKDLQVGMKL
                     PGVVTNVTAFGAFIDIGVHQDGLAHVSQLSDTFVRDAAEAVKVGQRVQATVMEVDIPR
                     KRIALSLKSKPDFERRQGGGGGGGQRPAGQSSGGQRGQSGGRPQGGGGFNNNPFGNLG
                     SGSSSSGGGDWFSQAASKGKR"
BASE COUNT         1616 a         3182 c         2824 g         1494 t
ORIGIN      
        1 agcaccagcg cctccaccag cagcccggcc gggatgcgcc agaaggccag ccagacggcg
       61 gcggcggcgc ccgcgggcac cgccaagccc gcgatccaga ggcgatgccg cagataaaaa
      121 cgatgccgct cgtccagctc cgcctccggc tgccccagcg tgtcgagcac acggtcgagc
      181 agataaatga cccacaccac cagagccagg ccgggcagca cgccgggcgg cagtttcgat
      241 ccgtgcaggc gcgccaggga tgccgtccac accaacgcca ccgccggtgc ctccaggctc
      301 agcatgtgcg cccacaacca gggcttggag aggagggatc gctcggacac gcgccatcag
      361 tggagcacgc gcgcggcggg tcaacctgga accatgccgc gaaacaaaag accggcttga
      421 gcaacccctc cgcctgcgcg attgggaggc aatgacgcgc caggaagcct gcctcgccct
      481 caacctgatc ccgcagatcg gcccggtgcg cgcgcgcaag ctcatcgccg ccttcggcgg
      541 accggagcag gtgctcaagg ccagtgcgcg ggacctcacg cgcgtcgaag gcatcggcga
      601 ggcgcacgcc caggccgtgg ccggatggga ggcgcaggtg gatttgcccg gcgagctggc
      661 aaaggtcaaa gcgcgcggca ttaccctgct gacagagact gacgagcttt tcccgccctc
      721 cctgcgcggc ctgcgcgacg cgcccctgct gctctatgtc tggggccagc tcctcccgcg
      781 cgaccaccat gccatcggca tcgtcggcag ccggcacgcc actgtttatg gactgagcgc
      841 caccaagcgc ctcggctacc agatcgcctt cgccggacgc accgtcatca gcggactggc
      901 gcgcggcatt gacaccgccg cgcacgaggc cgcgctcgcc gccaaaggcc gcaccatcgc
      961 cgtcatcggc tccggcatcg gcaaactcta cccgcccgag aatcaagccc tcgccgggcg
     1021 catcgcccag agtggcgcgg tcgtcagcga ataccccgtg gaccgcatcg ccgacaagca
     1081 gacctttccc taccgcaacc gcatcgtcgc cggctggtcc gcgggcatgc tcgtcgtcga
     1141 ggcgcccgcg cgcagcggcg cgctcatcac cgcccagcag gccgtggaca atggcaggac
     1201 cgtctatgcc gtgcccgggc ccatagacaa acccacctcc cagggctgca accgcctcat
     1261 ccagcagggt gccaagctcg tcatcgaggc ggaggacatc ctcgccgacc tcgacaccct
     1321 tttcccgccc gcgcacatcc cgcaggacgc gcgggccgat aacgctccgc ccgccgcgcc
     1381 gcccgtcagc cttgatgagg agatcgtcct caaggccgtg aaggacggag ccgcccacat
     1441 agacgaaatc atcaccgcct ccggtcttac ccccgccacc gtcaacgtct gcctcatgcg
     1501 cctggaaatg aaacgtctcc tgcgcgccct gcccggcaga tgctacgaag ccgccggctc
     1561 ctcctgaaaa agggaattcc acaccaggca aagccgtgaa cttccgcctt cttcgcctcg
     1621 caccctttct catcacgctc gtcatgggcg tgctgctgct gctgctggac tggctgatga
     1681 atgaaaaagg cgggctggaa ttcatccagc ggtttgaact ggccagttac gactggcgga
     1741 tgcgccagat ggacccgccc gcggacgcgg gacgccaggc gggcgtgctg catgtggatg
     1801 agcgcggcac ggagcggctg aaacaaaccc tgaaagtgaa ctggccggtg ccgcgctgga
     1861 ttcatggcgt gatccttgac gagctgaccg cgcagggcgc gctggcggtt ggctacgaca
     1921 tttttttcac ccgcatgcgt ccggaggaca aggcgccggc gatgagcacg gcggcggctc
     1981 catcatccga cgaggtcttc atccaggcgc tcaggcggag cgggcgcacc gtgctgggcg
     2041 cgccgatgga cccctcccgt tcgggagaca atgtgctgct gccggcggcc cccttcgcac
     2101 aggccgccgc cactgccggg cacgcgctgg gaaaagccga cccggacggc gtgctgcgcc
     2161 gcgtgctgcc gctggtcgag gatgcctcgg cggggcgtgt ctggcagttg ggcatccaga
     2221 ccgccgcttt ggccctgggt ctggatttga accgggcgca actcacgccc tggcggctga
     2281 cggtgccgga tgcgcaaggc aggacgcatt acatcccgct cgacgggcat ggcaggctgg
     2341 tcattccctg gcggctgtcc gcgacaccgg cggcctccgc gcgcaaccgc tccttcttcg
     2401 atgtgctggg cgactcgcag cggcgcagga gcggccaggg cggcgggcct tcgtcgtttt
     2461 ggcaggaccg tgtggtgatc gtcggctcct tcggggcaaa tccgccgctg gccgatgccg
     2521 gacccactcc gctcggagcg cgcacgcctt actgcgccac gcactggaac atcgccgatg
     2581 cgatgatgcg cggagccttc atccatgtgc ccggaccagc aacgcgggcg ggcgtcactc
     2641 tggcgctgat gctggctgtc agcgtgcttt cctggaagct gcgcgccctg tgggcgacgg
     2701 cggcggcggc gcttctcagc gcggcctatg ccgcctgctg cgtctggctg ctgcacgcgc
     2761 ggggactgtg gctgccgctg gcgctgccgc tgctgggcgc cacgagcatc acgcatgtga
     2821 cgatgacgtt tttccggctc gtcatcgaaa aagggcacaa gcgccgcctg cgcagcgtct
     2881 tccagcagat cgtctccccg cgcctgctgg agatgattct cgaccagccg cacgtctcat
     2941 gggctccgca ccagaagatg atgacggttt tcttcgccga catccgcggc ttcaccgcgc
     3001 tcacagacga ctcccgcctc gaccgcatcg gcctcacgcc tgggcaggag ccgcccgatc
     3061 ccgcgcagtc gcaggccctc cgcgaccggc atgccctcga caccgtcaat ctctatctcg
     3121 gactcgtcgt ggacacgatt aaaaagcacg gggccacgct cgacaaatac atgggggact
     3181 gcgtcatggc tttttggggc gcgccgctcg acgaccgcca tcatgcgctc aaggccgtcc
     3241 gcgccgccgt ggaggtgcag cgccgcatcc atgatctcaa tgagcggcgc cggatggaaa
     3301 acgagcgcat cgggcaggaa aaccgcgcgc gcatggcgga cgggcgtccg cctcttcagc
     3361 cgcatgcgct gctgactttg ggcaccggca tcaacaccgg cctgatgacc gccggcttca
     3421 tgggatccga ggcgcacctc tccaattaca ccgtctttgg ccgcgaggtg aacctcgccg
     3481 cgcggctgga gtccctctca ggcagcagcc gtattttgat ttcccaggcc acgctcgacg
     3541 ccgtcagcgc ggacgacccg gcgctggccg ccagcgcgcg cggcatcggc gcgcatccga
     3601 tgaagggaat ccagcagcct gtgccggttt atgaagtgtc ctgggaggaa acgcggaaaa
     3661 ataacaacga agcgcaggcg ggcgcgttat agggcgcgga acaaatgggg accacccaat
     3721 atccacaccg tcatgaaacc gaaatccaag aaaaaggcgg caaacaaaaa agcggcgccc
     3781 aggcgcgcca ttcagaaacg cggcgcgctg aaaaaaaaga ccaccagtgc cgcagcaaag
     3841 aaactgacct ccccggcacc caagccaaag ccggcagtca aagccccgcc ccctgtcatc
     3901 aagggcggga ttcgggagta cttcgtcgtc cgggccgcaa agacacccgc cgcgctgctt
     3961 tcacctttga cttccaacgg caagccgagg atcagcctca acgagggtga aatctggatc
     4021 gaagactcgg atggcgtcac cactcccgaa actctccctt acgagctgga gtcgggcgac
     4081 accatctgca cctacaatcc gggggatgac cccagtgatc ccgccaccct tcccgaaatc
     4141 cagagcttcg aagtggactg ctacgagtcc gacggcagct actatgagga cgtcatcgtc
     4201 tatgactact ttgatgactg catctcggtg gatttaaccg tgctcgacga gtgcatcgag
     4261 gagcctgagc cggccaccga cgaagacccc tacacctgca atgacggcac cttcagcatc
     4321 ggcatcgaag gctgacctcc catcacttca aacccatcac cctccgccca ccatgccact
     4381 ttaccttagc gttctgaaat tgaccggcag cgtcacctgg agaaacggca ccaccggcac
     4441 cttcatcccg ctgaacaact gccagagctt cgacaccgcc gccacggcca ccgcccagtt
     4501 ccgcctgcgc accaacgacg cctccattga gattcaccag ttcgaaagcc ccatgcagtc
     4561 cccgccctgc cccgtccccc acaaaggcag caccatgatc atggtcctga accgcgaccc
     4621 ggcgaatctg aaaaagcgcc gcgggaatct caccccccgc tcgcgcaaag ccctcggagc
     4681 cagcggcaaa aggcgcgaac tcaaaagagg gagcgccacc ctgcaatccg tccagaccgg
     4741 cggggtcaca ttggaaatcc gaacctgagc gcctgtccgc actgcgcgga gtttgccgca
     4801 gtgacaccgg tgccggcggc cgcttcacta agcgggcgga acaaaccccc gccccggatg
     4861 cgttttcccg ccgtcatgaa aacgccgctc atcctcctca ccctcgcctc cgccgccttt
     4921 gcccagccgc cgaacatcct cctcgtcctc gccgacgacc tcggctacgg cgacctcgcc
     4981 tgctacggag ccaaagacct gcgcaccccc catctcgacc gcctcgccgc cgagggactg
     5041 cgcttcaccg actgctacgc cggccacgcc aactgctccc cctcccgcac cgccctcatg
     5101 accgggcgca cccccacgcg cgtcggcgtg cgcaactgga tccccgaaga ctcccccgtc
     5161 cacctccgcc gcaccgagac caccctcccc accctcctgc gccaggccgg ctacaccacc
     5221 tgcctcagcg gcaagtggca tctcaacggc gccttcaacc acccctccca gccccagccc
     5281 ggcgaccacg gatttgacca ctggttcgcc acccaaaaca acgcctaccc cagccaccgc
     5341 aaccccgcca acttcgtccg caacggcacc cccctcggca aactcgaagg ctacgccgcc
     5401 cacctcgtgg ccgacgaagc cctgcgctgg ctcgacgcgc gcgacaagtc caagcccttc
     5461 ttcctctaca tcgccttcca cgagccgcac gagcccatcg cctccgacgc ccgatacacc
     5521 gccctctacc cccacgaaga ccccgcctac agcgcccacc acggcaacat cacccagatg
     5581 gacgacgcct tcggccgcct catggccaag ctcgacaccg acggactgcg ggagagcacc
     5641 cttgtcttct tcaccagcga caacggcccc gccatcaccg cccagcaccc ctacggctcc
     5701 gccggccccc tgcgcgacaa aaaaggctcc ctctgggaag gcggcatccg cgtccccggc
     5761 atcctgcgct ggcccggcaa agtccagccc ggagccacca gcgccgaacc cgtcagcggc
     5821 gtggacctcc tgcccaccct ctgcgccgcc gccggcgtca ccccgccgca ggaccgcgcc
     5881 ctcgacggag ccagcatcct ccccgcgctc gaaagccgcc ccgtcacccg ctccaccccg
     5941 ctttactggc acttcaaccg cgccagcggc gagcccaaag ccgccctgcg agcgggcgac
     6001 tggaaaatcc tcgccaccct cgacaaagcc cccgccaccc gcaccaacga catcaccgag
     6061 caggacgagc gcgacttcaa agaagccagg ctgcaaaact tcatgctcca caacctgcgc
     6121 gccgaccccg ccgagcagcg cgacctcgcc gccgccgagc ccgccaaact cgccgaaatg
     6181 aaggccctcc tggaagccaa atacgccgaa gtccaggccg aatcccccac ctggcccgcc
     6241 tggaccttca ccggcgccga aggcaaaaag atcgaatggc ccgactatgt gaggaaaaag
     6301 aaagccgccc cgaaaaagca gccctgagcc gtggaagcgg cgtcccgccg cttttcgccc
     6361 cctttgcaca cggagacctc tttctccacc gggcttttgg agagggtttt tgggcctttg
     6421 gaggcgcttc accaagctct ggagacggtt tttggggctt tggtgacgct tcaccacgct
     6481 ttggagacga tttttggagc tttggaggcg cttcaccaga ctttggtgac ggttcaccaa
     6541 gctttggtga cgcgtctcca gacttcccgg agctttccgg acgctttggt gacgcgtctc
     6601 cactcttttc agaaagctcc aggagctttg gtgacgcgtc tccaaaccgt ggagaatgct
     6661 ctgggaactt tggtgacgcg tctccaaacc gtggagaatg ctctgggagc tttggtgacg
     6721 cgtctccaaa ccgtggggaa tgctcccgga gacgtggtga cgcgcctcca aacgctggaa
     6781 aatgcttccg aaaccgtggt gaggcgtctc caaaccgtga agaaagcgct ggaatgtccg
     6841 gggatgcgtc accccggcgc ccggaaagcg ctggaaagtg ggtccgcccg cctccacggg
     6901 tctcaaaacg ctccggggaa gccgccgggg cgtctcagga gaggcgctgt ggcgggtttg
     6961 gacgtggagg cgcagaaagc agcggagatc ggggtggacg acaagtctcc agagtaatgt
     7021 aagaattccc cagtgaccga ttacaacgaa gctgaaaaag tcgccaggaa gcgatttttt
     7081 ttcagggatg gggtgtgcgg cagcgtcggc gcgccgcctc agcccccgct gggtgcggct
     7141 gcgggaaagg cgttgctctc ggcgggatgc tgccctctca gcgcttcccc ttcgaggcgg
     7201 cctggctgaa ccagtcgccg ccgccgctgc tggagctgcc gctgccgagg ttgccgaagg
     7261 ggttgttgtt gaaaccgccg ccgccttggg gtctgccgcc gctttggccg cgctggccgc
     7321 cgctgctctg gccggcgggt ctttggcctc cgcctccgcc gccgccctgg cggcgctcga
     7381 agtcgggctt ggatttgagg ctgagggcga tgcgcttgcg cgggatgtcc acctccatga
     7441 cggtggcctg gacgcgctgt ccgactttga cggcctcggc ggcatcgcgc acaaaagtgt
     7501 cgctgagctg gctgacgtgg gcgaggccgt cctggtggac gccgatgtct atgaaggcgc
     7561 cgaaggcggt gacgttggtg acgacgccgg gcagcttcat gccgacctgg aggtccttca
     7621 tttcattgac gccttcctgg aatttgaata cctcgaactg cttgcgcggg tcgcgcccgg
     7681 gcttggcgag ctcgctcatg atgtcctgga gcgtgggcag gccgacatcg gcgctgacgt
     7741 attttttgag gtcgagcttc tggcgcagct cgggccgcgc catgaggtcg gcgacggtgc
     7801 agccgaggtc ggcggccatt tgctcgacca gggggtagcg ctcggggtgg acggcgctgg
     7861 agtcgagcgg gtgggcggcg tcgcggatgc gcaggaagcc ggcggcctgc tcgaaggctt
     7921 tgtcgccgag gcgcgggact ttgagcaaat ctttgcggga tttgaagggg ccgttttcgt
     7981 tgcggaaggc gacgatgttc gccgcgtggg tgctgttgag tccggagacg tagctgagga
     8041 gctgcttgga ggcggtgttg acctcgacgc cgacgttgtt cacgcaactg atgacgatgt
     8101 tgtcgaggcc gtccttgagg agcttttgat ccacgtcgtg ctggtattgg ccgacgccga
     8161 tggatttggg gtcaattttg accagctcgg ccagggggtc catgaggcgg cggccgatgg
     8221 agacggcgcc gcgcacggtg acatcgtggt tggggaactc atcgcgcgcc acttcgctgg
     8281 cgctgtagat ggaggcgccg ctttcattga ccatgatgac ggggatctgc ttgggcacgc
     8341 cgattttgtt gatgaaagcc tcggtctcgc ggctggcggt gccgttgccg atggcgatgg
     8401 cctcgatctt gaagcgctcg accaaattca gcacgagggt cttggcgtgc atgaggctgt
     8461 tgccctcgcc gaggaggtag atgacgtcgt tgaaaaggag ctggccctgg gcgtcgagca
     8521 cgacggtttt gcagccggtg cggaagccgg ggtcaatgcc gagcaggcgc ttctggccga
     8581 gcggggcggc catgagcagc tcccgcaggt tgtcggcgaa gactttcacg gcttcggcgt
     8641 cggctttctt ttttgactca aggcggaact cggtctccat gctggggccg agcaggcgct
     8701 tgtagctgtc ggcgcaggcc agcttcatct gctcggtgca ggtgttgccg cctttggcga
     8761 acatgtgggt gacgagctgc gtggcggctt cctcgggcgg ctggatgcgc atgagcagga
     8821 agccctcttt ttccccgcgg cggatggcga gcatgcggtg gctggggatg gatttgagcg
     8881 gctcggtcca gtcgaagtaa tcgcggaatt tctgcgcgtc ggcctcggtc tccttgccga
     8941 acatgacctt ggaaagcacg gtggcctgct cggcgaagag ttttctcaac gcggcgcggg
     9001 cctcggcatt gtcgctgatg cgctcggcga tgatgtcgcg cgctccggcg agggcgtcgg
     9061 cggcgtcttt gacgcgcagc tcctcatttt cgtggtcggg gttgatgaac ttggcg
//
LOCUS       DAOMYJ010000204         9068 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Prosthecobacter sp. isolate AMR_MDS_5629
            NODE_2551_length_9068_cov_22.385998, whole genome shotgun sequence.
ACCESSION   DAOMYJ010000204 DAOMYJ010000000
VERSION     DAOMYJ010000204.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867850
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prosthecobacter sp. (wastewater metagenome)
  ORGANISM  Prosthecobacter sp.
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Verrucomicrobiaceae; Prosthecobacter.
REFERENCE   1  (bases 1 to 9068)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9068)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.30
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:34:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,742
            CDSs (total)                      :: 4,684
            Genes (coding)                    :: 4,676
            CDSs (with protein)               :: 4,676
            Genes (RNA)                       :: 58
            tRNAs                             :: 55
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9068
                     /organism="Prosthecobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2551_length_9068_cov_22.385998"
                     /isolate="AMR_MDS_5629"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1965333"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..689)
                     /locus_tag="PLP58_17395"
     CDS             complement(<1..689)
                     /locus_tag="PLP58_17395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ10358.1"
                     /translation="MPSRRLTPFLPAIFLAAALAWSGWMVVSRQQARLPGEWRVLLPG
                     ALPELHPFLPADEAERQILDLLHEPLIRLDRQGRPAPALAEEWRWHLRVSCWFADAAR
                     AQAGRRALEAVPAETRLEWGLDEARIEENALALRFAKPGTPGAGEALRLLAGHSPQGT
                     TFWRVETWPEARDVMREFAAHPEHEVKRIWFDDNGACEIVTTLGQAAALAVLADWLRP
                     RLPRLPETRALG"
     gene            798..1685
                     /locus_tag="PLP58_17400"
     CDS             798..1685
                     /locus_tag="PLP58_17400"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HRJ10359.1"
                     /translation="MRIHTLDLLFQDTPGLIASHLLETREGLALVETGPGSTLAALRA
                     AIRAHGFDEKDVRHVLVTHIHLDHAGAAGWWARQGARVYCHPNAARHLIDPSRLMASA
                     RMVYGDDMDRLWGEMQPAPVEQVTVLQDGEILRLGGAEITAWDTPGHARHHHAFVCGD
                     ICFTGDAAGVRLGGSSYQGVTSAPPQFEPEAYDATLARLLAGEFRQIYLAHFGVVNDV
                     RAHLEAYRVRIRQVHERMSAWVAEGRDSETTRRLHQDAENALALSQGVTGELWQSYEK
                     ANGTAMCADGIRLCVEKSA"
     gene            complement(1697..2353)
                     /locus_tag="PLP58_17405"
     CDS             complement(1697..2353)
                     /locus_tag="PLP58_17405"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HRJ10360.1"
                     /translation="MNPAAVSLIVSKEPLVRLGMRHFLMESAGISVCLEACGSMDAMR
                     QLMEHKPALVIVFACDRPLEVVRLVRELRRKRRSQPVLVVSRTAEEDHVRRCLDAGVL
                     GYTTTGDDLNELRLACAAVMRGDMHLSKSAARVIQGANGHSKNWHGGMPASGTKALTV
                     REVEVFRLMGSGLGCKELAAQLGISVKTVETHQMRLKEKLQARSCDELRNLARARREN
                     "
     gene            2681..3298
                     /locus_tag="PLP58_17410"
     CDS             2681..3298
                     /locus_tag="PLP58_17410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ10361.1"
                     /translation="MLQKILLNGSECLRNQRLRELAAVLALCSPVTGQDLPQPVSPDV
                     SKPLPQIQRIESPKDFIEAFKEASKRMSKSDWEAVRKAQEDAIDPAGAKKKAPLPDPG
                     REQVPGALADAEAAANSLRMMREWYSLKPGGALPNMVPGPPQIPASAYLAELRGLESK
                     VARLEEEVTLLKKANEQKQEIITLQEQFIVDLKKQIEVPAKENQP"
     gene            3310..3837
                     /locus_tag="PLP58_17415"
     CDS             3310..3837
                     /locus_tag="PLP58_17415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ10362.1"
                     /translation="MPESSKNAEGPALDSLEKMIAAKKQEAAATDRTLAAPLSSTSAA
                     AGTTGATIKTVQNEQPWTPALVGGLSLGLMVFGVFIFCLITHLVKEGKVTEPAGDGQW
                     LLRLMVVPLCVLAAVLLVIVGFSNEQTAPAVGLLGTIIGYLLGSGARGVAPVGATAVQ
                     QGATPSPPVKPSSGT"
     gene            4003..4485
                     /locus_tag="PLP58_17420"
     CDS             4003..4485
                     /locus_tag="PLP58_17420"
                     /inference="COORDINATES: protein motif:HMM:NF024929.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HRJ10363.1"
                     /translation="MPAPARFPRQAGVRAFYGPNGVRGGYTPPTTTIRLPFTIYYDAQ
                     AVNTLSPHRLCAQSLLNVFNRLAAAFPTRAERQAAGILTYNGLYNPRRMRGNSGAWSM
                     HAWAIAIDLNAGSNGNMTHWPARATMPLEVMECFAAEGWLPAGAFWSRDAMHFQATAP
                     "
     gene            4517..5296
                     /locus_tag="PLP58_17425"
     CDS             4517..5296
                     /locus_tag="PLP58_17425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ10364.1"
                     /translation="MRTLSLLGLITAAALSCSCKSGERKPNLWPRFLGSGLTGSAPAQ
                     HLLEARRDSLIGQLTKQAERPSRTDSEMDGSGASPQLKQHASIFQIRYEQAQTDAAAR
                     NELVWDLIQIADVNYQLYEQGLVAKLQGGNAIINIGTAGMNAASTLSGSSAVKTTIST
                     LTTFITGSQAEINKTIFRDMAIQTMISNMRALRAKRYQVILTKLRKHHNDAGKYPVGE
                     AMKDVLDYYQAGTFVSAMIETQNSAAQVENEAKEATDKPAT"
     gene            5410..6342
                     /locus_tag="PLP58_17430"
     CDS             5410..6342
                     /locus_tag="PLP58_17430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ10365.1"
                     /translation="MMLCQCASYERFYDRLQEGEFKGKYELVKTGGTDPMNRAEIHIL
                     RQKASAPFHFTRSGEDGEVIEPMRMKTSGGSIPYAVQVLVKRNENYLPYTFERSYFIH
                     DWLFEAHKHKLSAHRRHTLQSAALVMAECQKTEVEEWRARQTFGTPEQAADHEKKLRD
                     KRQAIWLQYQAVKSFIAKKMWDGAPEPRPGGPDYEPEQVTTVNTSVVQRVDVQIADER
                     QRPLAENLRARMLALGLKSHGVENVVRTGSSEPPLRTEVRYHNEGDMELALYLQEILS
                     NPRHFGEVRVVKAPPDAGAERRRQLEVWFPRIRA"
     gene            6394..7875
                     /locus_tag="PLP58_17435"
     CDS             6394..7875
                     /locus_tag="PLP58_17435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ10366.1"
                     /translation="MKLSSTLGKTISDFCENKFTNPADNHCAHYVCHVLEVTSGYTCK
                     AHTGGKGPAACLRVQELFAVCPQVGVWKDVPGDLCLVFVTDNANVDLKHHTMRNVPKK
                     HVGILSGGQVYNYGNTSDTVVSQTPGDFLKRFQKTYGGDQALFFGTFPAGARTPEPES
                     GAAVPASAGAPAPESAHAATSSRLQIRPQTSGEKTDYFARLDGGPEYYVARSVSYLTY
                     RGLHQPGKMQSGPRYRTSDFKPQYETVAGVLGVISAGESSGFFNRLNSYDRAAFTFGF
                     FQLAAHTPNDNLILFFRRLAAENSAFQDLFPELKVVDGVLHRLIGRHGVSLERPSPRP
                     GHPSEMNLRDFMSYLNPEASKVDDQELSIAARLVHLADSDPGFNSLQVNVAAGITMRK
                     MRDRYAQWYSLDGMSDLVCTAIADIHHQGRGTKTEVRSALASSSTVSGKVAALCLIGE
                     SKYASRCATLKKALKDAEKDGHLGISVYDKASGLFRPATGWPD"
     gene            complement(7957..>9068)
                     /locus_tag="PLP58_17440"
     CDS             complement(7957..>9068)
                     /locus_tag="PLP58_17440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009962973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="helicase-related protein"
                     /protein_id="HRJ10367.1"
                     /translation="VGENPQKAALRSGADIVIACPGRLLDLMGQGCADFSGLEHLVLD
                     EADRMLDMGFLPDIRRVIKQLPSKRQTLLFSATLSKEIEAITREFQHAPKIVQIGKRS
                     NPAETVTQFVYEVPRHLKLALLAHLLEDEKLQMVLVFSRTKHGADKIARKLEKGGVKC
                     ATLHANRSQNQRLRALEDFKSWKVRVLVATDIAARGIDVDGISHVVNFDFPHVVEDYV
                     HRIGRTGRAQAIGDAISFVSTDEMDDLRRLERFIGRGLVRKRAEGFDYSAAATTPPPE
                     EDFRDRPRQQGGGRGGRGGQRQNTPRGGQRGQRHDPKSHSGSSQETWRPSARNDNARS
                     ESRSQGSKPQGRARQGDTPANPGAARKLWNRLTGR"
BASE COUNT         1833 a         2844 c         2729 g         1662 t
ORIGIN      
        1 cccagggcgc gtgtctccgg caggcgcggc agtcgcgggc gcagccagtc ggccagcacc
       61 gcgagcgcgg ccgcctgccc gagggtggtg acgatttcac aggcgccgtt gtcatcgaac
      121 cagatgcgtt tcacctcatg ctccgggtgc gcggcgaact cccgcatcac gtcccgtgcc
      181 tccggccagg tctcgacgcg ccagaaggtc gttccttgcg gcgagtgccc cgccagcagc
      241 cgcagcgcct cgcctgctcc gggtgtgcca ggcttggcaa agcgcagcgc gagcgcgttt
      301 tcctcgatcc gcgcctcgtc gagtccccac tccagccgcg tctccgccgg caccgcctcc
      361 agagcgcgcc gtcccgcctg cgcgcgcgcg gcatcggcaa accagcagct cacccgcaga
      421 tgccagcgcc actcctccgc cagcgccggt gccgggcggc cctggcggtc cagccggatg
      481 agcggctcgt gcagcaggtc cagaatctgc cgctccgcct catccgccgg caaaaaggga
      541 tgcagctctg gcagcgcgcc cggcagcagc acgcgccact cccccggcag ccgcgcctgc
      601 tgcctggaga ccaccatcca gccggaccaa gccagagccg ccgccagaaa aatggcgggc
      661 agaaatggcg tcaggcggcg tgaaggcatt cgaaaaaagt atcgcgcgcg ggaggatcgg
      721 aaaatcgaat ttcgaatttc gaatttgcga tttccagcct acgcctgcac ccgccggccg
      781 ccgctttttc ccagtccatg cgcattcaca ctctcgacct gcttttccaa gacacccccg
      841 gactcatcgc ctcccatctg ctggagacgc gcgaagggct ggcgctcgtg gaaacgggac
      901 ccggctccac tcttgcagcg ctgcgcgccg ccatccgcgc gcatggcttt gatgaaaagg
      961 acgtgcgtca tgtgctcgtc acccacattc acctcgacca cgcgggggcg gcgggctggt
     1021 gggcgcggca gggagcgcgc gtgtattgcc atcccaatgc cgcgcgccat ctcattgacc
     1081 cctcgcgcct gatggccagc gcgcgcatgg tttatggcga tgacatggac cgtctctggg
     1141 gggagatgca gcccgcgccc gtggagcagg tgacggtgct tcaagacggt gaaatcctgc
     1201 gcctcggcgg tgcggagatc accgcctggg acacacccgg ccatgcccgg caccatcatg
     1261 cctttgtctg cggagacatc tgcttcactg gcgatgccgc cggcgtgcgt ctcgggggct
     1321 ccagttatca gggagtgacc agcgcgccgc cgcagttcga gccggaggcc tatgatgcca
     1381 cgctggcgcg cctgctcgcc ggagaattcc gccaaatcta tctggcgcat tttggcgtcg
     1441 ttaacgacgt gcgcgcccat ctggaagcct atcgcgtccg catccgccag gtgcatgagc
     1501 gcatgagcgc ctgggtggcg gaggggcgcg actccgaaac cacccgccgt ctccaccaag
     1561 atgcggagaa cgccctggcc ctcagccagg gcgtgaccgg agagctatgg caaagctacg
     1621 aaaaagccaa cggcaccgcc atgtgcgccg acggcatcag gctgtgtgtg gaaaagagcg
     1681 cgtgagaccc cgcggctcag ttttcccgcc tggctcgcgc cagattcctg agttcatcac
     1741 acgagcgcgc ctgcagtttc tctttgagcc gcatctggtg ggtctccact gttttcacgc
     1801 tgatgcccag ttgcgcggca agttccttgc aacccaggcc gctgcccatg agacgaaaca
     1861 cctccacctc ccgtaccgtc aaggctttgg taccggatgc cggcatcccg ccatgccaat
     1921 ttttcgaatg accgttcgcc ccctgaatca cgcgcgccgc gcttttgctc aaatgcatgt
     1981 ccccccgcat gaccgccgcg cacgccaggc gcagctcgtt gagatcatcc cctgtcgtgg
     2041 tgtagccgag cacacccgcg tcgagacagc ggcgcacatg gtcctcctcc gccgtgcggc
     2101 tgaccacgag cacgggctgg ctccggcgtt tgcgccgcag ttcccgcacc aggcgcacca
     2161 cttccagagg ccggtcgcag gcaaagacga tcaccaaagc cggcttgtgc tccatcaact
     2221 ggcgcatcgc atccattgag ccgcaggcct ccaggcagac gctgatgccg gcggactcca
     2281 tcaggaaatg ccgcatcccc agcctgacca gcggctcttt ggagacgatc aatgacacag
     2341 cggcggggtt catgacatga actggaaacg acagcgggat gcctttgcat ccctggcaga
     2401 atgacaatgg aagaccgcgt ttaaccgttg ccgccccgtt cgggcaagca caaaaatcag
     2461 ggaatcggga aagattcttg agacaacacc tgtcctggct ggcggaatat tggacttggc
     2521 ggggcattga tgggcgtttg gatgcaggcg gcggaaagcc caaacattgc ccctgttccc
     2581 tgacagcatt cctcgaagcc ggcggcagaa aaatcaggag caaccctgat ggaaaccatc
     2641 cgagctccgg caaagaatga gccaacttag ctttctccgc atgctccaga aaatcctgct
     2701 caatggatct gaatgcctcc gtaaccaacg gcttcgtgaa ttggccgcag tcctggcatt
     2761 gtgcagtccc gtgacaggcc aggatctgcc tcagccagtc tcccctgatg tttccaagcc
     2821 tttgccgcaa attcagcgca tcgaatcccc caaagacttt attgaagcat tcaaagaggc
     2881 atcaaaaagg atgagcaagt cagactggga ggctgtgcgc aaggcgcagg aagatgcgat
     2941 tgatccagcc ggggcgaaga aaaaggctcc cttgccggat cccgggcgcg agcaagttcc
     3001 tggcgcgctc gctgatgccg aggcagcggc gaattctctg aggatgatgc gcgaatggta
     3061 ctcgctaaag ccgggcggcg cattgcccaa catggttccc ggcccacccc aaatccccgc
     3121 atccgcctat cttgcggagc tacggggttt ggaaagtaag gtcgcaaggc ttgaggagga
     3181 ggtcacattg ttgaaaaaag ccaacgagca gaagcaggag atcatcactc tgcaagagca
     3241 gttcatcgtt gatctgaaaa agcagatcga agtcccggcc aaggagaatc aaccctgaac
     3301 cattgactca tgcctgaatc atccaaaaat gcggaaggac cggcgctgga ttccctggag
     3361 aaaatgatcg ccgccaaaaa acaagaggct gcggcaaccg atcggaccct tgcggcgcct
     3421 ctgtcctcaa ccagcgctgc tgcgggaacg actggggcaa cgataaagac cgttcaaaat
     3481 gagcagccct ggacacccgc gcttgtgggg gggctgagtc ttggcctgat ggtcttcggt
     3541 gtctttattt tctgcctgat cacgcacttg gtcaaagaag gcaaggtcac cgaaccggcg
     3601 ggggacgggc aatggctgct caggctgatg gtcgtgccgt tatgcgtgct ggccgccgtt
     3661 ttgctggtca ttgtgggatt ctccaatgag cagacggcgc ccgcagtcgg actgctgggc
     3721 accatcatcg gctatctgct gggttcggga gcgcgcggtg tcgcgcctgt cggggcaacg
     3781 gcagtccagc aaggtgccac acccagcccg ccggtgaaac caagctccgg gacatgatgc
     3841 cctctgttat tccgtcagaa caacacatcc accactcaaa cctcatccac caccatgact
     3901 caggcacaaa tccgcgccat gcaggcaaaa atcggcgccg aagttgacgg cttttggggg
     3961 ccgcgaagca tcgccgcctg tcagaggcat cttagatctt taatgcccgc gcccgcgcgc
     4021 ttcccgaggc aggcgggcgt gcgggccttt tacggaccga acggggtgcg cggcggttat
     4081 actccgccga ctaccaccat tcgtctgcca ttcacgatct attacgacgc gcaggcggtg
     4141 aacacgcttt ccccccatcg tctgtgcgcg cagtccctgc tcaacgtctt caaccggctc
     4201 gccgccgcct ttccgacacg cgcggagcgc caggctgccg gcattctaac ttacaatggt
     4261 ctttacaatc cgcgcagaat gcgcggcaac agtggagcgt ggtccatgca cgcatgggcc
     4321 attgccattg acctgaacgc gggcagcaac gggaacatga cacactggcc tgcccgcgcc
     4381 accatgccgc ttgaggtcat ggaatgcttc gccgcggagg gctggcttcc cgccggtgct
     4441 ttttggagcc gtgatgccat gcatttccag gccacagcac cgtgacactc ttcacagttc
     4501 gtttgcccgc accttcatgc gcaccctatc ccttcttggc ctcatcaccg ccgccgcgct
     4561 gtcttgctca tgcaaatcgg gtgagaggaa gcccaacctc tggcccaggt tcctcggcag
     4621 cggtctcacc ggcagcgcgc ccgcgcagca tctgctggaa gcgcggcgcg acagcctcat
     4681 cgggcagctc accaaacaag cggagcgacc cagccggaca gattctgaaa tggacggaag
     4741 cggcgcatct ccacaactca agcagcacgc ctcgatcttc cagattcgct acgagcaagc
     4801 ccagaccgat gctgccgcgc gcaacgaact cgtgtgggac ctgatccaga tcgccgacgt
     4861 gaactaccag ctctacgagc agggtctggt ggcgaaactc cagggcggca acgccatcat
     4921 caacatcggc accgcgggca tgaacgcggc cagcaccttg agcggcagca gcgcggtgaa
     4981 aacgacaatc tcgactctga ccaccttcat caccggctcc caggccgaga ttaacaagac
     5041 cattttccgg gacatggcca tccaaaccat gatctccaac atgcgggcac tccgcgccaa
     5101 aaggtatcaa gtcatactca cgaagctgcg caaacaccac aatgacgccg gcaaataccc
     5161 cgtcggcgag gccatgaagg acgtgctcga ctactatcag gccggcacct ttgtcagtgc
     5221 catgattgag acgcagaatt cggcggcgca ggttgagaac gaggccaaag aggccacaga
     5281 caagcccgcc acctaaaaga tgccgagaaa actcaagccc ggccaggaag gaacaaggca
     5341 ctccaagccc gcatgaaaca ccctccgcga aaacagcgat ctccgttcat tccagctgcc
     5401 gccgcaggca tgatgctctg ccagtgcgcg tcttatgagc gcttttacga ccggttgcag
     5461 gagggggagt tcaaaggcaa atacgagctt gtgaaaacgg gcgggacgga ccccatgaac
     5521 agggcggaaa tccacatttt gcggcaaaaa gccagcgccc ctttccactt cacccgctcc
     5581 ggcgaagacg gggaagtgat cgagccgatg aggatgaaaa ccagcggagg cagcatcccc
     5641 tacgcggtgc aggttcttgt caaaaggaac gagaactacc tgccttacac ctttgaacgc
     5701 tcctatttca tccacgactg gctttttgag gcgcacaagc acaagctctc cgctcacagg
     5761 cgtcacacgc ttcaaagcgc cgctcttgtc atggctgaat gccagaaaac cgaggtcgag
     5821 gaatggcgcg cgcggcagac tttcggcacg cccgagcaag ccgctgatca tgagaaaaag
     5881 ctgcgggaca agcgtcaggc catctggctt caataccagg cggtcaaaag tttcatcgca
     5941 aaaaaaatgt gggatggcgc tcccgaaccc aggccgggag gtccggatta tgagccggag
     6001 caggtgacca cggtgaacac ctccgtggtt cagagggttg acgtgcagat cgccgacgaa
     6061 aggcagcgcc ctttggctga aaacctgcgc gcgcgcatgc tggcgctggg attgaaatcc
     6121 cacggagtgg aaaacgtggt gcgaaccggc tcttccgagc cgccgctgcg gaccgaggtg
     6181 cgctaccaca acgagggtga catggaactg gcgctctatc tccaggagat tctctccaat
     6241 ccaaggcact ttggcgaagt ccgcgtcgtc aaagccccgc cggacgctgg cgcggagaga
     6301 cggcggcagt tggaggtctg gtttcccagg atccgtgcat gacctgccgt tcaaaatttt
     6361 caagacagtc aacaaactca ggcaagcagc atcatgaaac tcagctcaac tctcggcaaa
     6421 accatctcgg acttttgtga gaacaagttt accaaccctg cggacaacca ttgcgctcac
     6481 tatgtgtgcc atgtgctgga ggtaacgtcc ggctacactt gcaaggcgca taccggcggg
     6541 aagggcccgg cggcctgcct gagggtccag gagctattcg ccgtctgccc gcaggttggc
     6601 gtctggaagg acgttccggg agacttgtgc ctcgtgtttg tcaccgacaa tgcaaatgtg
     6661 gacttgaagc atcacaccat gcgcaatgtg ccaaaaaaac atgtcggcat cttgagcgga
     6721 ggccaggtgt acaattacgg caacacttcg gacacggtgg tttcgcagac tcccggggat
     6781 tttttaaagc ggtttcaaaa gacctatgga ggtgatcagg cgctcttctt tggcactttt
     6841 cccgcggggg ccagaacgcc ggaacccgaa agtggcgccg cagttccagc gagcgctggc
     6901 gcgccagcgc cggagtccgc gcatgcggca acaagttcca ggcttcaaat tcgccctcag
     6961 acatcggggg aaaagaccga ctattttgcc aggctcgacg gcggacctga atactatgtg
     7021 gcgagatcgg tcagttatct gacctaccga ggtctgcatc agccgggtaa aatgcagtcg
     7081 gggccgcggt atcggaccag tgatttcaaa cctcaatatg agacagtcgc aggcgtgctc
     7141 ggagtgattt cggcaggcga aagcagcggt tttttcaacc ggctcaacag ctatgatcgc
     7201 gcggcattca cttttggctt tttccagctc gcagcgcata ctccgaatga caatctcatc
     7261 ttgttttttc ggcggcttgc ggcggagaac tccgcttttc aggatttgtt cccggaactc
     7321 aaggtcgttg atggtgtcct tcaccgcctg attggcaggc atggtgtgag tctggagagg
     7381 ccatcaccac gtcccggcca tccaagcgag atgaacctgc gtgacttcat gtcctacctt
     7441 aatccggagg cctccaaagt ggacgaccag gagctgtcga tcgccgcgcg tcttgtgcat
     7501 ctggctgaca gcgatccggg cttcaactcg ctgcaggtca atgtggcggc cggcatcacg
     7561 atgagaaaga tgcgtgaccg ctatgcccaa tggtacagtc tggacggcat gtcggacctc
     7621 gtctgcaccg ccatcgcgga catccaccat caagggcgtg gcacgaagac cgaggttcgc
     7681 tcagcattgg catccagcag cacagtgagt ggaaaagtcg ccgcgctgtg cctgatcgga
     7741 gagtcaaaat acgcctcgcg ctgcgccact ttgaaaaagg cgctcaaaga tgcggagaaa
     7801 gacgggcacc ttggcatttc agtctatgac aaggcaagcg gactgttccg accggcgacg
     7861 ggctggcctg actgaggggg cggccttgtt gtcgggcatt cctggagtca gcaggctcaa
     7921 tatattctcg gcgtggcaaa tgaaccaggg tcgccctcac cttcccgtga gcctgttcca
     7981 gagtttgcgt gcggcgccgg ggttggcggg ggtgtccccc tggcgggcgc ggccctgggg
     8041 tttggagccc tggctgcggc tttcggagcg cgcgttgtca tttctggcgc ttggacgcca
     8101 tgtctcctgg cttgagccgc tatgactttt cggatcgtgc cgctgcccgc gctgtccgcc
     8161 gcgtggtgtg ttctgccttt ggcctcccct gcctccgcgt ccgccgccct gctgccgggg
     8221 gcggtcgcgg aagtcctcct cgggcggcgg agtggtggcg gcggcactgt agtcgaagcc
     8281 ctcggcgcgt ttgcgcacca ggccgcggcc gatgaagcgc tccagccggc gcaaatcgtc
     8341 catttcatcg gtgctgacaa aactgatggc gtccccgatg gcctgagcgc ggccggtgcg
     8401 gccgatgcgg tggacatagt cctcgacgac gtgggggaag tcgaaattca cgacgtggga
     8461 aatgccgtcc acgtctatgc cgcgcgcggc gatgtcagtg gcgacaagca cgcgcacctt
     8521 ccatgatttg aaatcctcca gcgcgcgcag gcgctgattt tgcgagcggt tggcgtgcag
     8581 ggtggcgcat ttcacgccgc ctttctccag cttgcgcgcg atcttgtccg cgccgtgttt
     8641 cgtgcggctg aaaacgagca ccatctgcag cttctcatcc tccagcagat gggcgagcag
     8701 ggcgagcttc aggtggcgcg gcacttcata cacgaactgg gtgacggttt cggcggggtt
     8761 cgagcgtttg ccgatctgga cgatctttgg cgcgtgctgg aactccctgg tgatggcttc
     8821 gatctccttg gagagcgtgg cggagaaaag cagggtctgc cttttggagg gcagctgctt
     8881 gatgacgcgg cggatgtccg gcaggaagcc catgtcgagc atgcggtcgg cttcgtcgag
     8941 cacgaggtgc tcgaggccgg agaagtcggc gcagccctgg cccatgagat caagcaggcg
     9001 gccggggcag gcgatgacga tgtccgcccc ggagcgcagg gcggcttttt gagggttctc
     9061 gcccacgc
//
LOCUS       DAOMYJ010000205         9018 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Prosthecobacter sp. isolate AMR_MDS_5629
            NODE_2568_length_9018_cov_22.847038, whole genome shotgun sequence.
ACCESSION   DAOMYJ010000205 DAOMYJ010000000
VERSION     DAOMYJ010000205.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867850
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prosthecobacter sp. (wastewater metagenome)
  ORGANISM  Prosthecobacter sp.
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Verrucomicrobiaceae; Prosthecobacter.
REFERENCE   1  (bases 1 to 9018)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9018)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.30
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:34:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,742
            CDSs (total)                      :: 4,684
            Genes (coding)                    :: 4,676
            CDSs (with protein)               :: 4,676
            Genes (RNA)                       :: 58
            tRNAs                             :: 55
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9018
                     /organism="Prosthecobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2568_length_9018_cov_22.847038"
                     /isolate="AMR_MDS_5629"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1965333"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(250..2595)
                     /locus_tag="PLP58_17445"
     CDS             complement(250..2595)
                     /locus_tag="PLP58_17445"
                     /inference="COORDINATES: protein motif:HMM:NF016071.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PPC domain-containing protein"
                     /protein_id="HRJ10368.1"
                     /translation="MNRILLPLVVYLLAGISHAQAPAASAKPAKPAKLELTSITPRAA
                     HIGGELLVSLAGKNFTEDTQAMASHPGLKVQLAKQSVKATSARLKITAGAEVPRGAYD
                     LWLKNASGESAKLKVYADEIQPTVTKAADFKEAPVSLAALPASVWGTLTETGQHDVYR
                     FTAKAGEELVLDLAVAQVGSVAKSPTLEIVDSNHTVLAVNRGLDSGSDPFLAWKAPAD
                     GDYHILVSNTTMDGSGGHVYRLTIGALPYVTGWSPLTAQVGKETKVTLIGHHLGDKAT
                     VTMMADKEGMTNVPLDAKALRFRTLPSLRVSALAQVSEVEGNDDLAKAQVVSSSSNIN
                     GLLAKAGDTDHFAFDAKKGQTWIIETLAAQAGSPADTKLEVLHPDGKPVARLLMQAVR
                     DSYNNFRSVDANNPDIRLQNWEEMELNEYVWFNGDVMKIIRMPRGPDGGCFFYVSDGK
                     RRAYFDTSATSHSLDEPCYVVEPYPLGSAIAANGLPIFTLNYQNDDSGDRKIGRDSRL
                     TFTAPADGRFVVRVTDTRGWGGERFVYSLAIRAPKPDFSVKLAGMNPTVMPGASVGFS
                     FRADRQDNFDDPIQIDITGVPAGYWATSPLIIEAGHDLISGSLHAMPDAAKDADWSKL
                     KITAKGKNITHDAGTFGKVTLGAKPKFIIVLEPDAGGKPVMRDPAAETKPLELTLAPG
                     QTVKAWLRAVREDNKDLLSLDVHGLPHGVIIDDIGLNGVQIRSGENERPIFFRAANWM
                     TECESLCHGAISSARNEHDSAGLQTSFPILMKIRKSGVAAR"
     gene            complement(2677..3780)
                     /locus_tag="PLP58_17450"
     CDS             complement(2677..3780)
                     /locus_tag="PLP58_17450"
                     /inference="COORDINATES: protein motif:HMM:NF025019.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HRJ10369.1"
                     /translation="MYSAWAEAPIAWLCGVRRAGKTTLAESLGVDQMLYLNCDLPSAE
                     EVVADPELFYRGCAKPVVVFDEIHQLRDPARVLKIGADAFPKLKILATGSSTLAASKK
                     FRDTLTGRKRLVHLTPVLWDELGVFDVTLQQRLFHGGLPPAMLAESKKPSLYREWLDS
                     FFARDIQRLFGFRDMNRFNMLFEYLLKQSGGQFEVTKTATALGITRPTVESHVSALEI
                     TQAMTLVRPFHGGGLNELVKQPKIYAFDTGFVSFARGWDPLRQEDFGLLWEHLVLEHL
                     QAHFPDTQPLYWRDKQGREIDFVLPRGRDAVDVIECKWSSKAFDGAALGVFRSYYPEG
                     KNYLLTPSTAPAHLKRFGPHEVMVCNPSALKPE"
     gene            complement(3883..5187)
                     /locus_tag="PLP58_17455"
     CDS             complement(3883..5187)
                     /locus_tag="PLP58_17455"
                     /inference="COORDINATES: protein motif:HMM:NF019262.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ10370.1"
                     /translation="MKRLLLLSIACLATTHAQTVDYTKQIAPLWDTYCVDCHSADDAD
                     GEFALDTFAALMKGGEEGAAIVAGKADESLLVKFLEGRSGRGGKNEFMPPGKREKLKE
                     EEIALIKAWINAGAKGPLVADAKPMPKEVITPKIAPTVPPKRSIQGVAFSPKAQLIAT
                     ARYGEVELVNPVNQVTVRKLTGFTGKVNAVAFSPDGETVYAAGGEAGIVGIVRSWKTS
                     DGTALRSFEGHTDAAYALAVSPDGKFIATGAYDQKIRLWDTATGKEVALLKGHNGAVN
                     GLSFRPDGKVLASASADRTVKLWSIPTGQRLDTLSQPTKEQTSVVFSADGKTLFAGGG
                     DNRIRVWSISAAAKEGTNKILTSRFAHEGGLLNLALSADGKLLASTATDKTLKLWNTA
                     DLTEKLLLEKQPDWSSALAFTDKAQLIAGRVDGTWSVYEAAR"
     gene            complement(5581..6219)
                     /locus_tag="PLP58_17460"
     CDS             complement(5581..6219)
                     /locus_tag="PLP58_17460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ10371.1"
                     /translation="MKTFFQTVLGLLFAGLGFFGTWAGVLTWTNMSHPWVTCLVALVI
                     SGACYLSTQEHRSRWLRFPAVGLLAGAGLLGGALSYARPVPLLCFGIVVFLLVALSVR
                     LKSIRKITKWFACLFALNWLIFAADHLFLSGQVFAWKWRVMEQPIFGPGGFIKVERHG
                     RRVIYATDAGFLDPWNVAIVWGWHVFPQVVSFGDKLPDGELLKDSKIQEAIR"
     gene            complement(6257..8458)
                     /locus_tag="PLP58_17465"
     CDS             complement(6257..8458)
                     /locus_tag="PLP58_17465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009958632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1553 domain-containing protein"
                     /protein_id="HRJ10372.1"
                     /translation="MQLRISSLLFAVLAATSAAVAEAPSFRNTIQPILTRYGCAMGAC
                     HGAAAGQGGFRLSLRGFDDEGDWLSITRSANGRRLTLEDPARSLFLLKAVKAVPHKGD
                     KRFEVNSPEYKAIVDWIAAGAPGPKADDPRIVSLSVSEPHLVSQAGKSVQLKVTAKFS
                     DGHSEDVTRWCKYNATNASVATVDDSGLIKITGAGEGTVSAWYLSLLSVTNVVVPKPG
                     KPDIEAFAALKPRNFIDEHVLTKLRELNIPPSGRATDAEFLRRAFLDTIGVLPTMDEA
                     KAFLADKAADKRDRLIDTLLKRPEFVDYWSHRWSDLFLVNGDKLMPQSMWSYYNWIRR
                     HVAANTPWDAMVRDLLTATGSTLEHGGGNFFILNDEPTKCAETVSVAFLGTSIGCAKC
                     HNHPMEKWTYDQYYAFANLFARVRTKNGTQTDERVLFSDTQGDLVAPLTGKPQPPRPL
                     DALQPVSMTSTEDRRITLANWLTSADNKLFQRAIVNRVWANFFGSGLVEAVDDLRVTN
                     PASNEPLFAAACEHLVKQKFDLKALMRTILQCETYQRSSITLPENISDLRYGSHYVPR
                     RLKAEVLLDTVSQVTAAPTTFKIDRRNANKGTADAYPMGFRALQLPDSNIASYFLKSF
                     GRADRVATCECERTNEPSMAQALHIANGETLNQKLAQQDNRLDALLTSQQPDAKIIEE
                     AYLLTLTRAPTDRELEKATALLTTAKPEDRRVTLEDLFWSLMSSKEFLFNH"
     gene            8488..8730
                     /locus_tag="PLP58_17470"
     CDS             8488..8730
                     /locus_tag="PLP58_17470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ10373.1"
                     /translation="MSTLAEIEIAAASLNLEEQQVLLARLAAKVRAHVTLPANQPRIP
                     GLHRGLVWMSDDFNDPLPDEFWLGKDSENSLKQPEP"
     gene            8727..>9018
                     /locus_tag="PLP58_17475"
     CDS             8727..>9018
                     /locus_tag="PLP58_17475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HRJ10374.1"
                     /translation="MRLILDTHAFIWWDAAPQKMSAKAYSACLDPGNTLHLSLASVWE
                     MQIKASLGKLHLRLPLADLIRDHQARNGLLLESIELPDILRLDSLPMHHRDPF"
BASE COUNT         1672 a         2689 c         2835 g         1822 t
ORIGIN      
        1 cagccatagg atttcctcgc ccgccacgtc ggcgagcaca tgccgccgca gaccgcactc
       61 ccgcgccacc gcgcgccagc ccgccatgag ggcgcgggtg tcatgggcct ggtggcaggg
      121 cagcagtggc ggcgaaggca tgggcaggga gtaggccctc cggagccggt gtgcaaaaac
      181 aaacctgcct caagcagcct ccatcccgga tttacgcgcc accatgccct gtgctcaacg
      241 aatgccggcc tacctcgccg ccactccgga cttccggatc ttcatgagga tcgggaagct
      301 cgtctgaagg ccggcgctgt cgtgctcgtt gcgcgcggag ctgatggcgc cgtggcagag
      361 gctctcgcat tccgtcatcc agttcgctgc gcggaagaag atggggcgct cgttttcgcc
      421 ggagcggatc tgcacgccgt tgaggccgat gtcgtcgatg atgacgccgt ggggcaaacc
      481 gtgcacgtcg aggctgagga ggtctttgtt gtcctcacga acggcccgca gccaggcttt
      541 gacggtctga ccgggtgcga gcgtgagctc cagcggcttc gtttcggcgg cgggatcgcg
      601 catgacgggc ttgccaccgg catctggctc caaaacgatg atgaacttcg gtttcgcacc
      661 gagcgtgacc ttgccgaagg tgcctgcatc gtgcgtgatg ttcttgcctt tcgcggtgat
      721 tttgagcttt gaccagtcgg cgtccttcgc ggcatccggc atggcgtgca gcgagccgct
      781 gatgaggtcg tgaccagcct cgatgatgag cggcgaggtg gcccagtagc ctgcgggcac
      841 gccggtgatg tcgatttgaa tgggatcgtc gaagttgtcc tgccggtcgg cacggaacga
      901 aaagccgacg gaggctccgg gcatcacggt ggggttcatg ccggcgagtt tgacgctgaa
      961 gtccggcttc ggcgcgcgga tggcaagcga atacacaaag cgctcgccac cccatccgcg
     1021 cgtgtcggtg actcgcacca cgaaacggcc atcggcaggc gcggtaaagg tcaggcgcga
     1081 gtcgcggccg attttgcggt cgccgctgtc gtcgttttga tagttcagcg tgaaaatggg
     1141 caatccattc gcggcgatgg cgctgccgag cggatacggc tccacgacat agcacggttc
     1201 atcgagcgag tgcgaggtgg cgctggtgtc gaagtaagcg cggcgtttgc catcgctgac
     1261 atagaagaag cagccgccgt ccggcccgcg cggcatgcgg atgatcttca tcacatcgcc
     1321 gttgaaccac acatactcgt tcagctccat ctcctcccag ttttgcaggc ggatgtccgg
     1381 gttgttggca tccacgctgc ggaagttgtt gtagctgtca cgcacggcct gcatgagcaa
     1441 acgggcgacc ggtttgccat cagggtggag gacttcgagc ttcgtgtcag cgggcgagcc
     1501 tgcctgcgcg gcgagcgtct cgatgatcca cgtctggccc tttttggcgt cgaacgcgaa
     1561 gtgatcggtg tcgcccgctt tggcgagcaa accgttgatg ttcgacgaag atgacaccac
     1621 ttgcgccttc gcgaggtcat cattcccctc cacctcgctc acctgcgcca gcgcactcac
     1681 gcgcagcgac ggcagcgtgc gaaaacgcag cgccttggca tcgagcggca cattcgtcat
     1741 gccttctttg tccgccatca tcgtcacagt ggccttgtcg ccgagatgat ggccgatgag
     1801 cgtgactttg gtctccttcc ccacctgcgc ggtgagcggc gaccagccgg tgacatacgg
     1861 caacgcgcca atcgtcaggc gataaacatg ccccccgctg ccatccatcg tcgtgttgct
     1921 cacgaggatg tggtaatcgc catcggcagg cgctttccac gcgaggaagg gatcgctgcc
     1981 gctgtcgagc ccgcgattca ccgcgagcac ggtgtgattc gagtccacaa tctccagcgt
     2041 cggcgatttc gccacgctgc cgacctgcgc caccgcgaga tcgagcacga gctcctcgcc
     2101 cgctttcgcg gtgaaacgat acacgtcatg ctgacccgtc tccgtcagcg tgccccacac
     2161 gctcgcaggc aaggctgcga ggctcaccgg cgcctctttg aagtccgctg ccttcgtcac
     2221 ggtcggctgg atctcatccg catacacttt gagcttcgcc gactcacccg aggcattctt
     2281 caaccacaga tcgtaagctc cacgcggcac ctccgcgcct gccgtgatct tcaaacgcgc
     2341 gctggtggcc ttcacgctct gtttcgcgag ttggaccttt aaacccggat gcgaggccat
     2401 cgcctgcgtg tcctcggtga agtttttccc cgccaagctc accagcagct cgccgccgat
     2461 atgtgcggca cgcggcgtga tgctcgtgag ttccagcttg gccggtttcg cgggcttcgc
     2521 ggacgctgct ggtgcctggg cgtgggagat ccctgccaaa agataaacga ccagtggtag
     2581 gagaatgcga ttcatgatgg ccgtactacg cctcaagacg cggtgatctc aatggacgat
     2641 gccgctttgg gtttgaagaa tttccttggg ggggattcac tccggcttca gcgccgaggg
     2701 gttgcacacc atcacctcat gcgggccgaa gcgcttcaga tgcgcgggcg cggtggaggg
     2761 tgtgagcagg tagtttttgc cttccgggta gtagctgcgg aagacgccca gggcggctcc
     2821 atcgaaggct ttgctgctcc atttgcactc gatcacatcc accgcgtcgc ggcctcgcgg
     2881 caggacgaag tcgatctcgc ggccctgctt gtcacgccag tagagcggct gcgtgtctgg
     2941 aaagtgtgcc tggagatgct ccagcacgag gtgctcccac agcaggccga aatcctcctg
     3001 ccgcagcgga tcccagcctc gggcaaagct cacgaagccg gtgtcgaagg catagatttt
     3061 cggctgcttc accagctcgt tcaggccgcc gccatggaat ggccgcacga gcgtcatggc
     3121 ctgggtgatc tccagcgcgc tgacatggct ctccaccgtg ggacgcgtga tgccgagcgc
     3181 cgtggccgtc tttgtcacct caaactggcc gccgctttgc ttgagcaggt attcaaacag
     3241 catgttgaag cggttcatgt cccgaaagcc aaagagtcgt tggatgtcgc gggcgaagaa
     3301 ggagtccagc cattcgcggt aaagcgacgg tttcttcgac tccgccagca tggcgggcgg
     3361 caggccgccg tggaagagcc gctgctgcag cgtcacatca aagacgccca gctcgtccca
     3421 cagcaccggc gtcagatgca ccagccgttt ccgtcccgtg agcgtgtcgc ggaatttctt
     3481 gctggctgcg agcgtggatg accccgtggc gaggattttc agctttggaa aggcatccgc
     3541 gccgatcttc agcacgcgtg ccggatcacg gagctggtga atctcgtcga aaacgaccac
     3601 cggtttcgcg cagccgcgat agaataactc cgggtccgcc accacctcct cggcggatgg
     3661 cagatcgcag ttcaggtaaa gcatctggtc cactccgagg ctttcggcca gagtcgtttt
     3721 gcccgcgcga cgcacgccac acagccacgc gatgggagcc tcagcccagg cggagtacaa
     3781 tcgttggagc caaaaaggac gctttatcat gccttcatac tatacaaatg gtaaaaccaa
     3841 atgcaaacca ttggagcgcc actcatggat tctcaaccga agctacctgg ctgcctcata
     3901 cacgctccaa gtgccgtcca cgcgacccgc gatgagttgg gctttgtcgg tgaaggcgag
     3961 ggcggaggac cagtcgggtt gtttttcgag gaggagtttt tcggtgaggt cggcggtgtt
     4021 ccagagtttg agcgttttgt ccgtcgcggt ggaggcgagc agtttgccat cggcagagag
     4081 cgcgaggttc aagagaccgc cttcatgcgc gaagcggctg gtgaggatct tgttcgtgcc
     4141 ttccttcgcc gcggcactga tcgaccacac gcggatgcga ttgtcgccac cgccggcgaa
     4201 aagcgttttg ccatcggcgc tgaagacgac gctcgtctgc tccttcgtcg gctgcgagag
     4261 cgtgtcgagt cgctgaccgg tcggaatgct ccacagcttc accgtgcggt ccgcgctggc
     4321 actggcgagc actttgccat cggggcggaa gctgagaccg ttcaccgcgc cgttgtgacc
     4381 tttgagcagc gcgacctcct tgccggtcgc ggtgtcccac aggcggattt tctgatcgta
     4441 ggcacccgtg gcgatgaatt tgccatctgg cgaaaccgcc agcgcatacg ccgcatccgt
     4501 gtggccttca aacgagcgta gcgccgtgcc gtcgctcgtt ttccagctcc gcacgatgcc
     4561 cacgatgccc gcctcgccac cggcggcata caccgtttcg ccatcgggcg aaaaggcgac
     4621 cgcgttgacc ttgccggtga agccggtgag tttccgaacg gtgacttgat tgaccggatt
     4681 gacgagttcg acctcgccgt agcgtgccgt ggcgatgagc tgcgccttcg gcgaaaacgc
     4741 caccccctgg atcgagcgct tcggcggcac ggtgggcgcg attttcggcg tgatgacctc
     4801 tttcggcatc ggcttcgcgt ctgcgacgag cgggcctttc gcgcccgcgt tgatccacgc
     4861 cttgatgagc gcgatctcct cctccttcag cttctcgcgc ttgcccggtg gcatgaattc
     4921 attcttcccg ccgcgacccg agcgcccttc gagaaacttc accagcagcg actcgtccgc
     4981 cttccccgcc acgatcgccg cgccttcctc gccgcccttc atcagtgccg caaacgtgtc
     5041 caacgcgaac tcgccatccg catcgtccgc gctgtggcaa tcgacgcagt aggtgtccca
     5101 cagcggcgcg atctgcttcg tgtagtccac ggtttgggcg tgggtggtgg cgaggcaggc
     5161 gattgagagg aggagaagtc gtttcatgac atcaaagaag ggtggaagca cgcgacagcg
     5221 tcctggagtg cgccggccct ccggcgcttt cgggtgtgtc cacggcgtgg ttaaaggcgt
     5281 gccagcgtcg atccggcgaa gcaaagtagt catctcgctc cgtcgagatg cgcgaatggc
     5341 tttcgccagc gggcatacgg tccgaatttc gggcaaccgt gagtttgcca tgcgcccagc
     5401 tcatctcgcg gagcgagatg gctactttgc tagcgcggct gtcgatcacg gtagatcccg
     5461 acggaacccc tttggggttc ggcgagatga aaacgcggga tgactggata cccagggttc
     5521 tgcgaaccct gggctgcatg acgcaagccc gttgggcttg aatgaagtta actgcacggt
     5581 tcatcgaatc gcctcctgaa tctttgaatc cttgagcagt tctccatcag gcagtttatc
     5641 accgaaactc accacctggg gaaagacatg ccatccccag acgatagcca cgttccaggg
     5701 atcaagaaac ccagcatctg tggcataaat aacacgccga ccgtgacgct ctactttgat
     5761 aaagccacca gggccaaaga tgggttgttc catcaccctc cacttccacg caaaaacctg
     5821 ccccgacaga aagagatgat cagcggcaaa aatcagccag ttcaacgcaa acaggcatgc
     5881 aaaccacttc gtgatcttcc gaattgattt taacctgaca gaaagtgcaa cgagaaggaa
     5941 gaccacaatg ccaaagcaaa gcaacggaac aggtctggcg taactcaagg ctcctccaag
     6001 caatccagct ccggctaaaa ggccgaccgc cgggaaccgt aaccagcgcg acctgtgttc
     6061 ctgtgtggac aaataacacg caccgctaat cacgagagcg acaagacatg taacccatgg
     6121 gtgcgacatg tttgtccaag ttagcactcc cgcccaagtc ccaaaaaatc ccagacctgc
     6181 gaacagcagc ccaagcacag tctggaaaaa ggttttcatc gtggtttgca gcgaatgtgg
     6241 acattcgctt tacggcttag tgattaaaca aaaactcctt cgagctcatc aggctccaaa
     6301 acagatcttc cagcgtgacg cggcggtctt cgggtttggc ggtggtgagc agcgcggtgg
     6361 ctttttcgag ttcgcggtcg gtgggggcgc gggtgagggt gaggaggtag gcttcttcga
     6421 tgatcttggc gtcgggttgc tggctggtga gcagggcgtc gaggcggttg tcttgttggg
     6481 cgagcttttg gttcaaggtc tcgccgttgg cgatgtgaag cgcttgcgcc atgctgggtt
     6541 cgttggtgcg ctcgcattcg caggtggcca cacggtcggc gcggccgaaa cttttgagga
     6601 agtagctggc gatgttgctg tcgggcagtt gcagggcgcg gaagcccatc gggtaggcgt
     6661 cggcggtgcc tttgttggcg ttgcgacgat caattttgaa ggtggtgggc gcggcggtga
     6721 cctgtgacac ggtgtcgagc agcacctcgg ccttcagtcg gcgtgggaca tagtgcgagc
     6781 cgtagcggag gtcgctgatg ttttcgggaa gggtgatgct gctgcgctgg taggtctcgc
     6841 attgcaggat ggtgcgcatg agggccttca gatcgaactt ctgcttcacg agatgctcgc
     6901 acgcggcggc gaagagcggc tcgttgctgg cgggattggt gacgcggagg tcatcgacgg
     6961 cttcgacgag gccgctgccg aagaagttcg cccacacgcg gttcacgatg gcgcgttgaa
     7021 agagtttgtt atcggcgctg gtgagccagt tcgcgagcgt gatgcgtcga tcctcggtgg
     7081 aggtcattga gacgggctgc aaggcatcga gcgggcgcgg cggctgcggt ttgccggtga
     7141 gcggcgcgac gagatcgccc tgcgtgtcgc tgaaaagcac gcgctcgtcg gtctgcgtgc
     7201 cgtttttggt gcgcacgcgg gcgaagaggt tcgcgaaggc gtagtattga tcgtaggtcc
     7261 acttttccat cgggtggttg tggcacttcg cgcagccgat ggaggtgccg aggaaggcca
     7321 cgctgacggt ttcggcgcac ttcgtcggct cgtcgttgag gatgaaaaag ttgccgccgc
     7381 catgctcgag cgtgctgccg gtggcggtga gcagatcacg caccatggcg tcccaaggcg
     7441 tgttcgcggc gacgtgacgg cggatccagt tgtaatacga ccacattgat tgcggcatga
     7501 gtttgtcgcc atttacgagg aagagatcgc tccatcgatg cgaccagtaa tcgacgaact
     7561 cgggccgctt cagtagcgtg tcgatgagtc ggtcgcgttt gtcggcggct ttgtccgcga
     7621 ggaaggcctt cgcttcgtcc atcgtcggca aaacgccgat ggtatcgaga aacgcacggc
     7681 gcaggaactc cgcgtccgtg gcgcgacccg atggcgggat gttcaactcg cgcagtttcg
     7741 tcaaaacatg ctcgtcgatg aagttgcgcg gcttcagggc cgcaaaggcc tcgatgtccg
     7801 gtttgcccgg tttcggcacg acgacgttgg tgacgctgag caggctcaaa taccacgcgc
     7861 tgacggttcc ttcgcctgcg ccggtgattt tgatgaggcc gctgtcatcc acggtggcaa
     7921 cgctggcatt ggtggcgttg tatttgcacc agcgtgtcac gtcctcgctg tgtccgtcgc
     7981 tgaatttcgc ggtgactttg agttgcacgc tttttcccgc ctgcgagacc agatgcggct
     8041 ccgacacgct gagcgacacg atgcgcggat catccgcctt cggccccggc gcaccggccg
     8101 cgatccaatc gacgatggct ttgtattccg gcgagttcac ctcgaagcgc ttgtcgccct
     8161 tgtgcggcac cgctttgacc gctttgagca gaaacagact ccgcgccgga tcttcgagcg
     8221 tgagacgacg tccattcgca ctccgcgtga tcgaaagcca gtcgccctcg tcatcaaaac
     8281 cgcgcaggga gagacggaag ccgccttgtc cagcggccgc accatgacag gcacccatcg
     8341 cgcaaccgta gcgcgtgaga atcggctgga tggtgttgcg gaaggacggg gcctcggcga
     8401 cggcagccga ggtggcggcc aggacagcaa aaagaaggct ggagatgcgg agttgcatga
     8461 atcaggggcg gaaattaaga tgccagcatg agcacactgg ctgaaatcga gatcgcggcg
     8521 gcttccctaa acttggagga gcaacaggtg ctgcttgcac gccttgcggc aaaagtacgc
     8581 gcacatgtga ctctgcccgc gaaccagccg cgcattccgg gactccatcg cggtctggtc
     8641 tggatgtcgg atgatttcaa tgatcccctg ccagatgagt tctggctggg caaggactcc
     8701 gagaacagcc tcaaacaacc cgagccatga ggctcattct cgacacgcac gcattcatct
     8761 ggtgggatgc ggctccgcag aaaatgtcgg ccaaggcata ctccgcctgc cttgatcctg
     8821 gcaacacgct gcatcttagt ctggcgagtg tctgggaaat gcagatcaag gcgagtcttg
     8881 gcaaattgca cctgagactg ccactggcgg atttgatccg tgatcatcaa gcacgaaacg
     8941 ggctgctgct cgagagcatc gaactgcccg atattctccg gctcgacagc ctgcccatgc
     9001 atcatcgcga tccattcg
//
LOCUS       DAOMYJ010000206         9007 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Prosthecobacter sp. isolate AMR_MDS_5629
            NODE_2574_length_9007_cov_22.067471, whole genome shotgun sequence.
ACCESSION   DAOMYJ010000206 DAOMYJ010000000
VERSION     DAOMYJ010000206.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867850
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prosthecobacter sp. (wastewater metagenome)
  ORGANISM  Prosthecobacter sp.
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Verrucomicrobiaceae; Prosthecobacter.
REFERENCE   1  (bases 1 to 9007)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9007)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.30
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:34:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,742
            CDSs (total)                      :: 4,684
            Genes (coding)                    :: 4,676
            CDSs (with protein)               :: 4,676
            Genes (RNA)                       :: 58
            tRNAs                             :: 55
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9007
                     /organism="Prosthecobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2574_length_9007_cov_22.067471"
                     /isolate="AMR_MDS_5629"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1965333"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1551
                     /locus_tag="PLP58_17480"
     CDS             <1..1551
                     /locus_tag="PLP58_17480"
                     /inference="COORDINATES: protein motif:HMM:NF020071.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase C-terminal
                     domain-containing protein"
                     /protein_id="HRJ10375.1"
                     /translation="QRAGRAGRLGPGIAVRLWPEREHLHRAESETPEIHRLDLSEALL
                     ALNVLGLGESFAWFEPPAAAALERARGLLHDLGAITVEGITETGRQMSRFPLHPRFAR
                     MLLAAADNHCVREAALCAAIAQGREILMAGKNESTHKNDDLWEPGDVSEFQAMLRAFT
                     RAEAMNFDPNACAPLRIHAMAAREAARSCEQFLRIARRAGLPVNDEVASPEALARTLL
                     AAFSDHLGIETSGGSRIYRLAGGYTGHLDKDAHIKAPRLLVAAEITEVQGRALTVRLN
                     HVTRVEEEWLRELFSADLTTGHRAVYDSTNRRVMNLEEVRFRSLVLSSREKGEPDEAT
                     AATLLADEVVAGRLTLVNWNDKVEQWITRVNCLSKWMPELEIPAIGEDDRRILIEQVC
                     HGCIAYRQLKDRDIFPALHQWLSHAQRDLLERYAPERLPLKNGRSARIVYDEKQPPSV
                     SVVLQHMYDVNENPKVAGGRVTVTIHLLSPAQRPIQTTGDIGRFWKEGYPAVKTQLRG
                     RYPKHEWR"
     gene            1602..2495
                     /locus_tag="PLP58_17485"
     CDS             1602..2495
                     /locus_tag="PLP58_17485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009960957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HRJ10376.1"
                     /translation="MTHPSFPDRRHFLTTLLGAGAAGFASAQGAALNYKGENIQFGLV
                     TYMWGADWDLPTLIANCQQVEVLGVELRIEHAHKVEPNLTAAQREDVRQRFEDSPVEV
                     LGMGTNCEFHSGDPDEVKKQIALARDYIKLSHDIGGSGVKVKPNNLPKDVPVEKTLEQ
                     IGKSLAELGDHALGFGQEIRLEVHGGVSDLGHIRTVMQAADRPNVRVCWNSNDQDLAG
                     EGIEVNFAKVQDYLGRTTHIREVNEGAYPYDKLAKLLVGVDYDGWVLLEARTKPADRV
                     AALAEQKKLFMELVTQARKAA"
     gene            complement(2845..3126)
                     /locus_tag="PLP58_17490"
     CDS             complement(2845..3126)
                     /locus_tag="PLP58_17490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009963425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SWIB/MDM2 domain-containing protein"
                     /protein_id="HRJ10377.1"
                     /translation="MPDKSKPKTARKLNPALAKPVQPDAVLAVVVGSAPLTRGELTKK
                     VWDYIKKHGLQDKTKKTNINADAALKAVFGGKSQVTMFEMTKLVSAHVK"
     gene            3446..4609
                     /locus_tag="PLP58_17495"
     CDS             3446..4609
                     /locus_tag="PLP58_17495"
                     /inference="COORDINATES: protein motif:HMM:NF019042.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
                     /protein_id="HRJ10378.1"
                     /translation="MASLFLMASVRGKDSKVDDLISSALSSHFLGDRLENLGLLHRDP
                     DHAWLQELWLLGRYHGQYHWSEGSAGDDVGFETRRLRMGGQARLFKKLTLHAQMVSGS
                     DIDPFYNGFTEMWVQWAFMPEMALTLGQQKHRFTHDRNVSSRYINYLERSMLTNMFGL
                     DYTPAVTLQGVIGPVTYYTGVFSNATGWDMGRAFTEFNSGWSCLAAVYYDLGKALPTD
                     TAHLHFTYLHSEADEQATNLDHFMDGFSTALILTQNSGSLVVESTVGTGNDQGDAFAL
                     NIQPGWYLTDNLQLVLRYQVAASNRAQGLIPQKRYEQSAGMQDGRVYQSGYFGLNFYL
                     AKHRLKLMTGIEYANMGGKDVWTASTMLRFFFGPHSGGPFPMNQMLHGFLDHD"
     gene            complement(4774..5550)
                     /locus_tag="PLP58_17500"
     CDS             complement(4774..5550)
                     /locus_tag="PLP58_17500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012419116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRJ10379.1"
                     /translation="MVKPAHECKDHVCWGAGQAHPHHHRLEVSELGVSYHRVLALEGI
                     SFATECGRSIALIGPNGAGKSTLLKTLAGLMPADGGSILWRGQPLTRSSHEIAYLPQR
                     GEVDWSFPITVRGLVEMGRYPNLGWWRQYSRHDADIVQRALTAMELLDLQDRQISALS
                     GGQQQRAFIARALAQEAHVLLLDEPFTGLDKPAQENLSRLFRELTAEGRLLIASHHDL
                     QTVAGIFDDVLLIKRRQVAFGPLGTAFTPENVALAYENQA"
     gene            complement(5544..5972)
                     /locus_tag="PLP58_17505"
     CDS             complement(5544..5972)
                     /locus_tag="PLP58_17505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ10380.1"
                     /translation="MRGFPPIQIFLIALAFGVLAFPLARLTGGGEVVMSANPESAAAA
                     ETPAYVRVRFAHPPQSVRLMQSGRDLLAGVDLSASPAETEAGIRISPDGDELQLDAEW
                     PEGTPDTALTIEIEPDGRETREQTRWSTGSTLSELLLFQW"
     gene            complement(6043..6942)
                     /locus_tag="PLP58_17510"
     CDS             complement(6043..6942)
                     /locus_tag="PLP58_17510"
                     /inference="COORDINATES: protein motif:HMM:NF013465.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding protein"
                     /protein_id="HRJ10381.1"
                     /translation="MKKLLALALLAASSLSAQAALKVATLHPLIADVVRTVGGDKVEI
                     VEVLKAGADVHHFEPGARDIAAMRGAGIIFASGKNLESYLGKLRDSVGPSVKVVEVGR
                     PIPSLKMEPGQDVFVCCPHHAHSAIDPHWWHSAENMRRAARVIADELAEADPANEKAY
                     EEGAKAAGNRFMELKKWAQQQIATIPRGDRKLVTAHAAFGYFCKEFGFKSIPVLGIGR
                     SDDVSSQYIAQAIQLIRDNKIRAVFPEDQANPKVLAEIVRSTGAKTGKPLVADGTATY
                     AHTFETMLAHNVRAIVEALGVKK"
     gene            complement(6999..8252)
                     /locus_tag="PLP58_17515"
     CDS             complement(6999..8252)
                     /locus_tag="PLP58_17515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ10382.1"
                     /translation="MIRIFPTLLLTALALGVHAQSPDQFRAQQQGRYTYTPFTAMSSP
                     VVLPMDGKSADWVTGLDVSLADMIFPHFHMDTAYGTASMDQEELAAGHHDPIRRDKFT
                     IQNLEFSLSGRLNDYNEFFVTYAGPFGEGDKYDGIWEEWFWKLKNLPGGFELRGGRVY
                     NRFGIQNTYHPHGFDWVDQYLVNTRMLGDDSLTTIGAEITWIAPLPWTSQFDFAIGVA
                     PGHEEHDHGGFTGRYNPDESHFGEDQTLVVANWTNIWNYNDFHQFRGGFSGAWGDNHS
                     GFRTSVYGAHFEYQWRQNGFEPGGRYFRVRSEAMFNRFRVEDEFGIRDNPAPQKDFGA
                     YLSFLYGMPNNLELGLRTEYVAGNHETEQDARFRASPGITWYANAARTLRLRLQYNYD
                     HSNMRGTDHAIWAQCSIAWGGPEVR"
     gene            complement(8355..9002)
                     /gene="purN"
                     /locus_tag="PLP58_17520"
     CDS             complement(8355..9002)
                     /gene="purN"
                     /locus_tag="PLP58_17520"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012420321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HRJ10383.1"
                     /translation="MHILPLVPGRSTTATLKRAATPEKSARLRLGVLGSGVGSNFQAI
                     LKAIQNGTLDAEVAIAISDQADSPFLETARAAGIHAQFVNPGANPRRLSDAAQKEIAE
                     HLQRAGVDLVVLAGFMKILRAPVLDAFSDRVINVHPSLLPRHKGADAPQQALDAGDAE
                     SGCTVHLVNAEVDSGRILAQARVPVLPGDTAETLQARIKKAEHKLLPGVLAEWQA"
BASE COUNT         1574 a         2644 c         3101 g         1688 t
ORIGIN      
        1 cagcgcgcgg gccgcgccgg gcgtctgggg ccgggcatcg cggtgcggct gtggccggag
       61 cgggagcatc ttcatcgcgc ggaaagcgag acgccggaga tccaccggct ggacctgagc
      121 gaggctttgc tggcgctgaa tgtgcttggc ctgggggaaa gtttcgcgtg gttcgagccg
      181 cccgcagccg ccgccctgga gcgcgcgcgc gggctgctgc acgacctggg ggccatcaca
      241 gtggagggca tcacggagac ggggcggcaa atgagccgct tccctttgca cccgcgcttt
      301 gcccgcatgt tgctggctgc cgctgacaac cattgcgtgc gggaggcggc cctctgcgcg
      361 gcgattgccc aggggcggga gattttgatg gcgggcaaaa acgagtccac gcacaaaaac
      421 gatgatctct gggagccggg ggatgtgagc gagttccagg cgatgctgcg cgcctttaca
      481 cgagcggagg cgatgaactt cgacccgaac gcctgcgcgc cgctgcgcat ccatgcgatg
      541 gcggcgcggg aggcggcgcg gagctgcgag caattcctgc gcatcgccag gcgcgcgggt
      601 ttgcccgtga atgacgaggt ggcctccccc gaggcactgg cgcgcacgct gctggcggcg
      661 tttagcgacc atctcggcat cgagaccagc gggggcagcc gcatttaccg actggcgggc
      721 ggttacacgg ggcatctgga caaggacgcg cacatcaagg ccccgcgcct gctggtggcg
      781 gcggagatca ccgaggtgca gggccgcgcg ctcaccgtgc ggctgaacca cgtcacccgc
      841 gtggaggagg agtggctgcg cgagcttttc tccgccgacc tcaccaccgg gcaccgcgcc
      901 gtgtatgaca gcacgaaccg ccgcgtgatg aacctggagg aggtgcgctt ccgctcgctc
      961 gttctcagca gccgggaaaa aggcgagccc gacgaggcca ccgccgccac gctgctggcg
     1021 gatgaggtgg tggcggggcg gctgactttg gtgaactgga atgacaaggt cgagcagtgg
     1081 atcacgcggg tgaactgcct ctcgaaatgg atgcccgagc tggaaattcc agccattggc
     1141 gaggatgacc ggcgcattct catcgagcag gtgtgccacg gctgcatcgc ctaccgccag
     1201 ctcaaggacc gcgacatttt tcccgcgctg catcaatggc tcagccacgc gcagcgcgac
     1261 ctgctggagc gttacgcgcc cgagcgcctg ccgctgaaaa acggccgcag cgcgcgcatc
     1321 gtgtatgatg agaagcagcc acccagcgtc agtgtggtgc tccagcacat gtatgatgtg
     1381 aatgagaacc cgaaggtggc gggcgggcgc gtcaccgtca ccatccacct gctctccccc
     1441 gcgcagcgcc cgatccagac cacgggagac atcggccgct tctggaagga gggctaccct
     1501 gcggtgaaaa cccagctgcg cgggcgctat ccgaagcatg agtggcggtg acttaaagat
     1561 tgccagcatt caagcgttgt tcttcccacc ctccttccgc catgacacat ccatcattcc
     1621 ccgaccgccg ccatttcctc accaccctcc ttggagccgg ggcggccggt ttcgcctcgg
     1681 ctcaaggcgc ggccttgaac tacaagggcg agaacattca attcggcctc gtcacttaca
     1741 tgtggggcgc agattgggat ctgcccaccc tcatcgcgaa ttgccagcag gtggaggtgt
     1801 tgggcgtgga gctgcgcatc gagcacgcgc acaaggtgga gccaaacctg accgccgcgc
     1861 agcgcgagga cgtgcgccag cgctttgagg actcgcctgt cgaggtgctc ggcatgggca
     1921 ccaactgcga gtttcacagc ggcgacccgg acgaggtgaa aaagcagatc gcgctcgcca
     1981 gggactacat caagctcagc cacgacatcg gcggcagcgg ggtcaaggtg aaaccgaaca
     2041 acctgcccaa ggatgtgcct gtggagaaga ccctggagca gatcggcaag tcccttgctg
     2101 aactcggcga ccacgcactc gggttcggcc aggaaatccg cctggaagtc cacggcggtg
     2161 tgagcgacct cggtcacatc cgcacagtga tgcaagcggc ggacaggccg aatgtgcgcg
     2221 tctgctggaa ctcgaacgat caagacctgg ccggcgaggg cattgaagtg aactttgcca
     2281 aagtccagga ctacctcggc cgcacgaccc acatccgcga ggtcaacgag ggcgcctacc
     2341 cttacgacaa gctggcgaaa ctgctcgtcg gcgtggatta cgatggctgg gttctcctgg
     2401 aggcccgcac caagccggcg gaccgcgtgg cggccctggc cgagcaaaag aagcttttca
     2461 tggaactggt gactcaggcg cgcaaggccg cgtgagacgg gcgcttgtgt cacgtgtccc
     2521 ggccttgttt ccgagactgg atgccgaccg gcgggaaggt tgcagccgcg cgcatgatgg
     2581 ttatccgccg cgctcaagcc cgccccggcg ccttgatcaa gcccgcctcc accggtttcg
     2641 aaatgcggtt gagcgcctta tacgaggatg tgaaggttgt gaattgaggc aggctggacg
     2701 acgcggcgcg ccttgtgcca ggtctgactc aattcgcagt gaaggagaaa aagctgacac
     2761 aaaagaatcc ccggcctgaa atggcatgcg gatcgcgtga acctcaatga cacttcaagg
     2821 ccggggagaa cgggcggagt tggattactt gacgtgcgcg gaaaccagtt tggtcatctc
     2881 gaacatggtc acctggctct tgccgccgaa gaccgccttc aatgccgcgt cggcgttgat
     2941 gttggtcttt ttagtcttgt cctggaggcc gtgtttcttg atgtagtccc acactttttt
     3001 ggtcaactcc ccgcgggtaa ggggagcgga gccgaccacc accgcgagaa cggcgtccgg
     3061 ttggacgggt ttggcgaggg cgggattgag tttgcgcgct gtctttggtt tggatttgtc
     3121 tggcatagga cttccaggct ggcggctttc tggccgctgg tcaaccctgg ggatgccccg
     3181 gattttgtgg gggctggata tttttaggat ggactgaaag cggggtttgc tttcttccag
     3241 tcgtcaaaaa atgaaaaaat caacatgcgc tagaacccgg atttggaggg aaggcgcctc
     3301 atattgtgac gctaatgtca cacaagactt ctcgctggac cgtcattcgg ttctcaatgt
     3361 gactccgacg acacaattca tgcgcatcaa gcccactatt tgtccaaacc gtcactttcg
     3421 actcccgttt ctgcctttga tcaccttggc aagtcttttc ctcatggctt ccgtgcgggg
     3481 gaaggattcc aaagtggatg accttatctc cagcgctctc agctcgcatt tcctcggaga
     3541 caggctggag aacctggggc tgctgcaccg cgatccggac cacgcatggc tgcaggaact
     3601 gtggctgctg gggcgctatc acgggcagta tcactggtcg gagggcagcg cgggggatga
     3661 tgtcggcttt gagacacggc gactgcgcat gggcggacag gcgaggcttt tcaaaaaact
     3721 gacgctgcac gcgcaaatgg tcagcggaag tgacattgac ccgttttaca acggcttcac
     3781 ggagatgtgg gtgcagtggg cgttcatgcc ggagatggcg ctgacccttg gacagcagaa
     3841 gcaccgcttc acgcacgacc ggaatgtctc cagccgctac atcaactacc ttgagcgctc
     3901 aatgctgacc aacatgtttg gcctcgacta cactccggcg gtgacgctgc agggagtcat
     3961 cgggccggtg acttattaca cgggagtttt ctccaacgcc acgggctggg acatgggccg
     4021 cgcgttcacg gagttcaact caggctggtc ttgcctggcg gcggtgtatt acgatttggg
     4081 caaggctctg ccgacggaca cggcgcacct gcatttcact tatctgcaca gcgaggccga
     4141 cgagcaggcg acgaatctgg atcacttcat ggacggcttt tccacggcat tgatcctgac
     4201 tcagaacagc gggtctctgg tggtggaaag caccgttggc acgggcaatg accaggggga
     4261 tgcctttgcc ctgaacatcc agccagggtg gtacctgacg gacaatctgc aacttgtgct
     4321 gcgttaccag gtggcggctt ccaatcgcgc gcagggtctc ataccgcaaa aacgctatga
     4381 gcagtccgcc ggcatgcagg atgggcgggt ctatcagtcg ggctatttcg gtctgaattt
     4441 ctacctggcc aagcaccgcc tgaagcttat gaccggcatc gaatacgcga acatgggcgg
     4501 caaggatgtg tggacggcca gcacgatgct gcgcttcttc tttggtccgc actcaggcgg
     4561 ccccttcccg atgaaccaga tgctgcacgg ctttttggat catgactgag cctgctgcaa
     4621 gcgcgtggcg cgggtgacgg gcatgggtct ggagtttgtg atgctgccgg caggaatcat
     4681 gcccggccgt ggatcgcgga acatggggcc cctcttgcgc aggctggctg ccgctcaaaa
     4741 cattcccggt catgaactgg ttcggttttc acctcacgcc tgattttcat aagccagcgc
     4801 cacattttcc ggcgtgaacg cggtgccgag cgggccgaag gcgacttggc ggcgcttgat
     4861 gagcagcacg tcgtcgaaga tgccggccac ggtttgcagg tcgtgatggc tggcgatgag
     4921 caggcggccc tcggcggtca gctcgcggaa gaggcgggag aggttttcct gcgcgggctt
     4981 gtccaggccg gtgaaaggct cgtcgagcag gagcacatgc gcctcctggg ccagggcgcg
     5041 ggcgatgaag gcgcgctgct gctggccgcc ggagagggcg ctgatctggc ggtcctggag
     5101 gtcgagcagc tccatcgcgg tcaaggcgcg ctggacgatg tcggcgtcat gccgggaata
     5161 ctgccgccac cagccgaggt tcgggtagcg gcccatctcc acgaggccgc gcacggtgat
     5221 ggggaagctc cagtccacct cgccgcgctg cggcaggtag gcgatctcat gactgctgcg
     5281 cgtgagcggc tggccgcgcc agagaatgct gccgccgtcc gcgggcatga ggccggccag
     5341 ggttttgagc agggtgcttt tccccgcgcc gttcgggccg atgagggcga tgctgcgccc
     5401 gcactcggtg gcgaagctga tgccctccag cgccagcacc cggtgataac tgacgcccag
     5461 ctccgacacc tccagccggt ggtgatgcgg atgcgcctgg ccggcgcccc agcagacgtg
     5521 gtctttgcat tcgtgggcgg gcttcaccat tggaagagga ggagttcgct gagggtggag
     5581 ccggtggacc agcgtgtctg ctcgcgcgtc tcgcgcccgt cgggttcgat ctcgatggtg
     5641 agcgccgtgt caggcgtgcc ttcgggccat tcggcgtcga gctggagttc atcaccgtcc
     5701 ggggagatgc ggatgccggc ctcggtctcg gcgggggatg cggacaaatc cacaccggcc
     5761 agcaaatcgc gcccactctg catgaggcgc acgctttgag gcgggtgggc gaaccgcacc
     5821 cttacataag cgggtgtctc agctgcggcc gcgctctccg ggttggcgct catcaccacc
     5881 tctccgccgc cggtgagccg ggccaatgga aaagccagca cgccgaaggc gagcgcgatg
     5941 aggaagatct gaatgggtgg aaagccgcgc atggccgctt gctgggatgg tggatcctca
     6001 atgcccgcga agccggggac aatcaagagc cgtgatggcg gctcactttt tcactccgag
     6061 ggcctcgaca atggcgcgca cattgtgcgc gagcatggtc tcgaaggtgt gcgcgtaggt
     6121 tgcggtgccg tcggccacga gaggtttgcc ggtcttcgcg ccggtgctgc ggacgatctc
     6181 ggcgagcact ttggggttgg cctgatcctc cgggaagacg gcgcggattt tgttgtcgcg
     6241 gatcaactgg atggcctggg cgatgtactg ggaggagacg tcatcgctgc ggccgatgcc
     6301 gagcacgggg atgcttttga agccgaactc cttgcagaag tagccgaagg cggcgtgggc
     6361 ggtgacgagc ttgcggtcgc cgcgcgggat ggtggcgatt tgctgctgcg cccatttttt
     6421 cagctccatg aagcggttgc cggcggcttt ggcaccctcc tcataggctt tttcattcgc
     6481 cgggtccgcc tcggcgagtt catcggcgat gacacgcgcc gcgcggcgca tgttttcggc
     6541 gctgtgccac cagtgcgggt caatggcgct gtgggcgtgg tgcgggcagc agacgaagac
     6601 atcctgcccc ggctccattt tcaaagaggg gatggggcgg ccgacctcga cgaccttcac
     6661 agaggggccg acgctgtcgc gcagcttgcc gaggtagctt tccaggttct tgccgctggc
     6721 gaagatgatt ccggccccgc gcatggcggc gatgtcccgc gctccgggct cgaagtgatg
     6781 cacgtcggcg ccggctttca agacctcgac gatctccact ttgtcaccgc cgacggtgcg
     6841 caccacatcg gcgatgagcg ggtgcagggt ggccactttc agcgcggcct gggcgctgag
     6901 gctggaggcg gcgagcaggg cgagggcgag gagttttttc attgggtcgg agggttcagg
     6961 gattcaaacg aagcgcgcgg cgtgatcttc atgcgcggtc agcgcacctc ggggccgccc
     7021 caggcgatgc tgcactgcgc ccagatggcg tggtctgtgc cgcgcatgtt ggagtggtcg
     7081 tagttgtatt gcaggcgcag ccgcagggtg cgggcggcgt tggcatacca ggtgatgccc
     7141 ggactggcgc ggaagcgggc gtcctgctcg gtctcgtggt tgccggccac atattcggtg
     7201 cgcaggccga gttcgagatt gttcggcatt ccgtagagga agctcaaata agcgccgaag
     7261 tccttttgcg gcgcgggatt gtcccggatg ccgaactcgt cctcgacgcg gaagcggttg
     7321 aacatggcct cggagcgcac gcggaaataa cgtccccccg gctcgaagcc gttctgccgc
     7381 cactggtatt caaaatgcgc gccatagacg ctggtgcgga agccggagtg gttgtcaccc
     7441 catgcgccgg agaagccgcc gcggaactgg tggaagtcgt tgtagttcca gatgttcgtc
     7501 cagttggcga cgacgagtgt ctggtcctcg ccgaagtggg actcgtccgg gttgtaacgc
     7561 ccggtgaagc cgccgtggtc gtgctcctca tgtccggggg ccacgccgat ggcgaagtca
     7621 aactgcgagg tccagggcag gggagcgatc caggtgatct ccgcgccgat ggtggtcagc
     7681 gagtcatcgc caagcatgcg tgtgttgacg agatactggt ccacccagtc gaacccatgc
     7741 gggtggtaag tgttctggat gccgaagcgg ttgtaaacgc gaccgccgcg cagttcgaag
     7801 ccgccaggca ggtttttgag cttccagaac cactcctccc agatgccgtc gtatttgtcc
     7861 ccctcgccga aggggccggc ataggtgacg aaaaactcgt tgtagtcgtt gagccggccg
     7921 gagagggaga actccaggtt ttgaatggtg aacttgtcgc ggcggatggg atcgtgatgc
     7981 ccggcggcga gttcctcctg gtccatggag gcggtgccat acgcggtgtc catgtggaag
     8041 tgcgggaaga tcatgtccgc caggctgaca tccaggcccg tgacccagtc ggcgcttttg
     8101 ccgtccatgg gcagcacgac gggcgaggac atggcggtga agggagtgta ggtgtagcgg
     8161 ccctgctgct gggcgcggaa ctggtcgggg ctttgggcat ggactccgag ggccagcgcc
     8221 gtgagcagga gggttgggaa tatgcggatc atgggagttg gtaatgacaa ctcaattgca
     8281 ctttctgcaa gcagcttttg gcaagtgatt tgcaggtgaa ggggtgtccc gccgcttgca
     8341 cttttcgcag cgcgtcacgc ctgccactcc gccagcacgc ccggcagcag cttgtgctcg
     8401 gccttcttga tgcgggcctg gagcgtctcc gcggtgtctc ccggcagcac cggcacgcgc
     8461 gcctgcgcca ggatgcggcc gctgtccacc tcggcgttga cgagatgcac ggtgcagccg
     8521 ctttcggcat cccccgcgtc gagcgcctgt tgcggggcgt cggcgccttt gtggcgcggc
     8581 agcagggagg ggtggacatt gatgacgcgg tccgagaagg cgtcgagcac cggggcgcgg
     8641 aggattttca tgaagcccgc cagcacgacg aggtccacgc ccgcgcgctg caagtgctcg
     8701 gcgatctcct tctgcgccgc gtcgctgagg cggcgaggat tcgcgccggg attcacgaac
     8761 tgcgcatgga tgccggcggc gcgcgcagtc tccagaaaag gcgagtccgc ctggtcggag
     8821 atggcgatgg cgacctcggc gtcgagtgtg ccgttttgga tggctttgag gatggcttgg
     8881 aaattcgagc cgacaccgga gccgagcacg ccgaggcgca ggcgcgcgct tttttccggc
     8941 gtggcggcgc gtttcaaagt ggctgtggtg gagcgtccgg gcacgagagg caggatgtgg
     9001 atggcgg
//
LOCUS       DAOMYJ010000207         8921 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Prosthecobacter sp. isolate AMR_MDS_5629
            NODE_2600_length_8921_cov_20.492669, whole genome shotgun sequence.
ACCESSION   DAOMYJ010000207 DAOMYJ010000000
VERSION     DAOMYJ010000207.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867850
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prosthecobacter sp. (wastewater metagenome)
  ORGANISM  Prosthecobacter sp.
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Verrucomicrobiaceae; Prosthecobacter.
REFERENCE   1  (bases 1 to 8921)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8921)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.30
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:34:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,742
            CDSs (total)                      :: 4,684
            Genes (coding)                    :: 4,676
            CDSs (with protein)               :: 4,676
            Genes (RNA)                       :: 58
            tRNAs                             :: 55
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8921
                     /organism="Prosthecobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2600_length_8921_cov_20.492669"
                     /isolate="AMR_MDS_5629"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1965333"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(153..1148)
                     /locus_tag="PLP58_17525"
     CDS             complement(153..1148)
                     /locus_tag="PLP58_17525"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase family
                     protein"
                     /protein_id="HRJ10384.1"
                     /translation="MRPPPRAILASLAVPFLLAACGTGQSRAPVASAALPVPAAVPVA
                     PAAGYAAALEGESPYRPSPYFLPEIDARPEFAASSVPYTRAESYLLIDAATGRHLASR
                     NAEVKRSVASTQKLVTALVILDAGNLDKPVRVLASDLNVEPTVLGLRAGETYTRRNLL
                     YVMLVKSCNDVANVLARDNAGSISAFAAKMNAKARSLGCYNSSFKNPHGLTAPGQYST
                     ARDMARVSMAAYRNPVIRDAVRRRSYTFRRNNGQMVTLKSTNDLLAFMPECNGMKTGY
                     TNAAGRCLISTASRSGRNVILVQLGTKTKYIWDDGRILMSWGLQRARGSSFAFAP"
     gene            complement(1257..2411)
                     /locus_tag="PLP58_17530"
     CDS             complement(1257..2411)
                     /locus_tag="PLP58_17530"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HRJ10385.1"
                     /translation="MQKRLHLVLLAALSLNAASRADQAVAPVLQPFVEQQILAGAVTL
                     VADKDKVLDVTTVGYADVAAQDAMSADDLFWIASMSKPITGAAFMILVDEGKVSVDDP
                     VEKYLPEFKGQMVAEKGGTKEKPTTGAPHAPNHPILIRNVLSHTSGLPFKSPMEQPTL
                     DQQPLAARVSSYAKLQLEFEPDTKYQYSNAGINTAGRIIEVVSGMSFEDFLQKRLLEP
                     CGMKDTTFWPDEAQLKRIAKAYKASEDKSGIEETHVAQLRYPLNDHAHRFPMPAGGLF
                     STAADCAKFCQMLLHGGTLNGKRILSEEAVKQMTSKQTAPGVENSYGFGFATGGGTFG
                     HGGAYSTNMTADPKSGLITVFMVQNAGWRSDEGKKIDGMFREAALKLFAK"
     gene            complement(2432..3364)
                     /locus_tag="PLP58_17535"
     CDS             complement(2432..3364)
                     /locus_tag="PLP58_17535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ10386.1"
                     /translation="MIKLASWIGAFALASALTALEAPRASYYKDGELHITVLGQPETK
                     ALTSGHWDFKPSWSITGDHLVFFRRLKDDPEVNNWITAMCIINTDGSGFHQLSDGTMT
                     DFNPTWTRDGKNTPVWNRKVPGKLSYHIMASKIGGKPGEEIQLTDPSYHAWVHSAVRD
                     GRLFVSAAPPKLGRGYYLMTPHPGGEPKFERVTCELAPKGILDRVSVSSSETKICFEY
                     QTGFKYAFPGRTLYMADFDVTQRTITNLKAFANAEGKKVWFAYPRFTKGETSIVYHAG
                     GKLFLYSLADGTTKQVSTEAKADYRYPHGEATPN"
     gene            complement(3368..4471)
                     /locus_tag="PLP58_17540"
     CDS             complement(3368..4471)
                     /locus_tag="PLP58_17540"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012371.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HRJ10387.1"
                     /translation="MQPLSNRLAVLAALVLPCLASFAAQPLPRSTPEAQGVSTQSIID
                     FVNELEKIDTLHSFMIVRHGKVIAEGWWSPEAADKPHVLWSLSKSFDSTAVGFAVAEG
                     KLSVDDPVLKFFPNEAPAEPSENLKAMKVRDLLTMSCGHEQELKIQNHNATAKEFLAH
                     PVPLKPGTHFQYNTYGSYMLSAIVTKVTGQTELDYLKRRLFDPLGIENPKWDASPEGP
                     SLGGYGLYLCTEDIAKFGQLYLQKGQWNGKQLLPASWVEQATSKQVPNEGKSHTKLGI
                     DWNQGYGFQFWRCTHDSYRGDGRAGQLCLVIPTKDVVVAMTAEANAFQQQLNAVWEKL
                     LPAFGDQALPEDAAAQAKLKEMASKLVAHPKKP"
     gene            complement(4662..8828)
                     /locus_tag="PLP58_17545"
     CDS             complement(4662..8828)
                     /locus_tag="PLP58_17545"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HRJ10388.1"
                     /translation="MISRTTLMSASDPSSSSREKEIFMAALDVDDPSAQAAYLDSACA
                     GDAELRARVERLLGLEADSRSFLQPPSVMRKGVEALEEPDFDATIQLAPAALPRQIVP
                     PPVSASSANPDSPVPFIFGRRIAQGGMGAILEASDCKLGRTIAVKIMLSEAACSEDQK
                     QRFIQEAAVLGRLEHPNIVPIHDLGRDSEGSLYYTMKLVKGRTLQHIIDDLRHEDKEA
                     LEHYTLDRLLTIFRKVCDALAFAHAQKIIHRDLKPENIMVGEFGEVLVMDWGIAKILD
                     DSRSGTCQVPSVTEAGLGKSRSLNEATPAQTLEGAVMGTPQYMSPEQALGQVTDMDAR
                     SDVFSLGGILYAILTLRPPVEGKDVWEVIEKVSTARIASPTTYGATTTGKGKSAVKGQ
                     VLEAKKITPLPHLPGGRVPNALSAVVMKALTLDKAKRYQDVAAFSADIEAFQGGFATT
                     AEQAGLAKQLALLIKRNKGIFTTAAAAWLLISGLAVWFIFNLRAKEQRATQAEVIAVE
                     ERETARKALAKSQLDLAEKEFERGKFVEAQKILGETPESFRDATWRFLQAHSTDYTSQ
                     LPLLDGGLAYGLRFVPPGDRLAARCWVGPSKVAGVGIFSLMGHQIGDFLLQTEYYPIT
                     FGIDREGGRVAQAVTANEVALWDLATRTVLHRWTSEIRAIERVLLSPDGATVLVAGER
                     KLIAYAAETGAPLWTQPFVGVVPSFSPDGQTVAHLASTKGLELQVQLLDPNTGEVRST
                     LEASADNPTKTTLQFNQAGDKFACLGGDELIMWNPRTAQKIRALHFPGETVIEMNPTG
                     DSVATNSVSSGRIRLWATKTGRLLRSFNGVQNEVLALAFSPDGMTLISTHRSFADGGA
                     YVWPTRLREEVSTIMHAFLEGRRVLFNADGSLVYAATAGDVGAWDPLTGKQKWNSQRT
                     TKGKIRDLAIHPAGESILYSEMGGRTSFTHVSTAGKRLEDFGTNQVSSLKFNRSGRLL
                     LTVAYAFTDTNPGNGFSMVEYPSGKELQKITFEKPGQPFAAFCLDDAAVATAALTGGI
                     TVWDWQAGKPLRQIDAAQTGSIACFAASPDGLHLATGGPDRWIRVWEAATGQLEAAFR
                     AHWEGVSSLEFSPDGREILSGGDDGSVRIHDASSGEERLAFYGLTTPVADVDISADGK
                     LIAAITRDEGITKVWDRQRSSAAARLKDQPATTKSAANQPAASQPAATSPALPKDADG
                     WEDLLAPLTPDEVEKNGNGWSLKDGELFSPAAKPHARLPLPGNYAATSYQLRVKLRQL
                     PASALFYLGFPIADRMCGFGLEGGGKYTGLILVNGKFGKDLPGVVEGKQVNDTEPHTF
                     EMTVRLAGANATITTTLDGKPLYEWTGPITDLSEASWWATTKPGALSLGSYAGGWAVS
                     EVKVKRLPVE"
BASE COUNT         1528 a         2662 c         2917 g         1814 t
ORIGIN      
        1 ccagggcacc ctttcaccac ccccagggca ccctttcacc acccccaggg cacccccctt
       61 gcaggcatgc aatccgctca aagacagact gttgcgtgaa gatgacgccc cgtagatggc
      121 aaggcagcac ttttgaacac tcccgcctcg ggtcacgggg cgaaggcgaa actgctgccg
      181 cgcgcgcgtt gcaggcccca gctcatgagg atgcggccgt cgtcccagat gtacttcgtc
      241 ttggtgccga gctggacgag gatgacattc cgcccggagc ggctcgccgt ggagatcagg
      301 cagcgtccgg cggcattggt gtagccggtt ttcatgccgt tgcactcggg catgaaggcc
      361 agcaggtcgt tcgtgctttt cagggtcacc atctggccgt tgttccggcg gaaggtgtag
      421 gagcggcggc gcacggcgtc gcgtatcacc ggattgcggt aggcggccat ggacacgcgc
      481 gccatgtcgc gcgcggtgga gtactggccg ggggcggtga ggccgtgggg gtttttgaag
      541 ctggagttgt aacagcccag ggagcgcgcc ttggcgttca tcttggcggc gaaggcggag
      601 atgctgccgg cgttgtcgcg ggcgagcacg ttggccacgt cgttgcagct cttcaccagc
      661 atgacgtaca aaagattgcg ccgggtgtag gtctccccgg cgcgcaggcc gagcacggtg
      721 ggctccacat tgaggtcgga cgccagcacg cgcacgggtt tgtccaggtt ccccgcgtcg
      781 aggatgacca gcgccgtcac cagcttctgc gtgctggcca cggagcgctt cacctcggcg
      841 ttgcgcgagg ccagatggcg cccggtggcg gcgtctatga gcaggtagga ctcggcccgc
      901 gtgtagggca cgctggatgc ggcaaactcc ggacgcgcgt ctatctccgg caaaaagtag
      961 ggcgacggcc ggtagggcga ctcgccttca agcgccgccg catagccggc cgcgggcgcc
     1021 accggaaccg ccgcaggcac gggcaaagcg gcggacgcga ccggcgcccg gctctgcccg
     1081 gtgccgcagg cggccagcag gaacggaacg gcgagtgagg cgaggatggc tctcggaggg
     1141 gggcgcatgg gaaaggaacg cggtggcagg acgcagattt cattccatcc ccgcgcgcgc
     1201 atttccagcc gcactttcgc aagtgagagt cgcggacagc aaatggagcc tgcgcctcac
     1261 tttgcgaaga gtttcagcgc ggcctcgcgg aacatgccat cgatcttttt tccttcgtcg
     1321 ctgcgccatc cggcgttttg caccatgaag acggtgatga ggccggattt cgggtcggcg
     1381 gtcatgttcg tgctgtaggc gccgccgtgg ccgaaagtgc cgccgccggt ggcgaagccg
     1441 aaaccgtagc tgttttccac cccaggcgcg gtttgcttgc tcgtcatctg tttcaccgcc
     1501 tcttcagaga ggatgcgttt gccgttcaag gtgccgccat ggagcagcat ctggcaaaac
     1561 ttcgcgcaat cggccgcggt ggagaaaagt ccgcccgcgg gcatgggaaa gcggtgcgcg
     1621 tgatcattca gcggatagcg gagctgggcc acgtgggtct cctcgatgcc ggatttgtcc
     1681 tcgctggctt tgtaggcttt ggcgatgcgt ttgagctggg cctcatccgg ccagaaagtg
     1741 gtgtccttca tgccgcaggg ctccagcagc cgtttctgca aaaagtcttc aaagctcatg
     1801 ccgctgacga cttcgatgat gcgcccggcg gtgttgatgc cggcgttgga atactggtat
     1861 ttcgtgtcgg gctcgaattc gagctggagc ttcgcgtagc tgctcacgcg ggcggcgagg
     1921 ggctgctgat cgagcgtggg ctgctccatc ggcgatttga aaggcagacc gctggtgtgg
     1981 ctgagcacgt tgcggatgag gatggggtgg ttgggggcgt gtggagcacc cgtggtcggt
     2041 ttttccttgg tgccgccttt ttcagcgacc atctggcctt tgaactcggg caggtatttc
     2101 tccaccgggt catccacgct cactttgccc tcatccacga ggatcatgaa ggccgcgccg
     2161 gtgatcggtt tgctcatcga ggcgatccag aagagatcgt ccgcgctcat cgcatcctgc
     2221 gccgcgacat ccgcgtaacc gacggtggtg acatcgagca ccttgtcctt gtccgcgacg
     2281 agtgtgaccg cgccagccag aatctgctgc tccacgaaag gctgaagcac aggcgcgacc
     2341 gcctggtccg cgcgagaagc ggcgttgagt gataaagcgg cgaggaggac gaggtggagg
     2401 cgtttttgca tgagaagggt gcggtgggga ttcagttcgg cgtggcttcg ccgtgcgggt
     2461 agcggtagtc ggctttggcc tcggtggaaa cttgcttcgt ggtgccgtca gcgagcgagt
     2521 agaggaagag tttgcctccg gcgtgataca cgatggacgt ttcacccttc gtgaagcgtg
     2581 ggtaggcgaa ccacaccttc tttccttccg cgttcgcgaa ggctttgagg ttggtgatgg
     2641 tgcgctgggt cacgtcgaag tccgccatgt aaagcgtgcg gccgggaaac gcgtatttga
     2701 agccagtttg atactcgaag cagattttcg tctcgctcga ggagacgctg acgcggtcga
     2761 gaatgccctt cggcgcgagt tcgcaggtca cacgctcgaa tttcggctcg ccgccgggat
     2821 gcggcgtcat gaggtagtag ccgcgaccga gcttcggcgg cgcggcggag acgaagagcc
     2881 gcccatcacg caccgcggag tggacccacg cgtgatagct tgggtcggtg agctggattt
     2941 cctcgccggg cttgccgccg atcttcgagg ccatgatgtg gtagctgagc ttgccgggca
     3001 ctttgcgatt ccacaccggc gtgttcttgc cgtcgcgtgt ccacgtcggg ttgaagtcgg
     3061 tcatggtgcc gtcgctgagc tggtgaaagc cgctgccgtc ggtgttgatg atgcacatgg
     3121 cggtgatcca gttgttcacc tccgggtcgt ccttcaggcg gcggaagaag acgagatgat
     3181 cgcccgtgat ggaccacgac ggcttgaagt cccaatgccc gctggtgagg gccttcgtct
     3241 ctggctggcc gagcacggtg atgtggagtt caccatcctt gtaataggac gcacgcggcg
     3301 cttccagcgc ggtcagtgcg gacgcgagcg cgaatgctcc gatccatgag gcgagcttga
     3361 tcatggttca cggtttcttg ggatgcgcca cgagcttgga ggccatttcc ttgagcttcg
     3421 cctgcgcggc ggcgtcttct ggaagagctt gatcgccgaa ggcgggcagc agcttttccc
     3481 acacggcgtt caactgctgc tggaaggcgt tcgcttcggc ggtcatcgcg accacgacat
     3541 ccttcgtcgg gatgacgagg cagagctggc cggcgcggcc atcgccacgg taggaatcat
     3601 gcgtgcagcg ccagaattga aagccatagc cctgattcca gtcgatgccg agtttcgtgt
     3661 gtgatttgcc ttcgttgggc acctgcttgc ttgtcgcctg ctcgacccag gaggcgggaa
     3721 ggagctgttt gccgttccac tggccttttt gcagataaag ctggccgaac ttcgcgatgt
     3781 cctcggtgca gagatagagc ccgtagccgc cgagcgacgg cccttcgggg ctggcgtccc
     3841 atttcgggtt ctcaatgccg agcggatcaa atagacgtcg cttcaagtag tcgagctcgg
     3901 tctggccagt gactttcgtg acgatggcgg agagcatgta gctgccgtag gtgttgtatt
     3961 ggaagtgcgt gccgggcttc aagggcacgg gatgcgccag gaactccttc gcggtcgcgt
     4021 tgtgattttg aatcttcagc tcctgctcgt gtccgcagga catggtgagc agatcgcgca
     4081 ctttcatggc tttgagattc tccgaaggct ccgcaggcgc ttcgttgggg aaaaacttca
     4141 gcaccgggtc atccacgctg agttttccct cggcgacggc gaatccgacg gcggtggagt
     4201 caaagctttt gctcaacgac cacagcacat gcggcttgtc ggctgcttca ggactccacc
     4261 agccctctgc gatcactttg ccatgccgca cgatcatgaa gctgtgcagc gtgtcgatct
     4321 tctccagttc gttaacgaag tcgatgatgc tctgcgtcga gacgccctgc gcctccggcg
     4381 tgctgcgcgg cagcggctgg gctgcaaagc tggcgaggca tgggagaacg agggctgcga
     4441 ggacggcaag gcggttggaa agcggctgca tggtcatggc gggtttggag aacgtgtttc
     4501 gacaaagaaa gtcgcaccga ttttcacatc ccgtgtggtg atccgatttt gcacgttgtt
     4561 cacgcttcag cgtgtttctt tgccttcact gacacgctga acaacgcact ctgcaacgct
     4621 cagccccagt gctctgtgcc ggaaaagtcc ctctaatcgc ttcattcgac aggcagccgt
     4681 ttcaccttca cctccgacac ggcccagccg cccgcgtaac tgccgagaga gagggcgccg
     4741 ggcttggtgg tggcccacca tgaggcctcg ctgaggtcgg tgatggggcc ggtccattcg
     4801 tagagaggtt tgccgtcgag cgtggtggtg atggtggcgt tcgcgccggc gagacgcacc
     4861 gtcatttcga aggtgtgcgg ctcggtgtcg ttcacctgtt tgccctccac gactccgggc
     4921 aggtctttac caaacttgcc gttcacgaga atcaggccgg tgtatttgcc gcctccttcc
     4981 aggccaaaac cgcacatccg gtccgcgatg gggaagccca ggtagaaaag agcactcgcg
     5041 gggagctgcc gcagcttcac gcgcagttgg tagctggtgg cggcgtagtt gccgggcagc
     5101 ggaagcctcg catgcggttt cgcggcaggg ctgaaaagct cgccgtcttt caagctccaa
     5161 ccgttgccgt ttttctcgac ctcgtccggg gtgagcgggg cgaggaggtc ctcccagcca
     5221 tcggcatcct tcgggagcgc aggcgaggtg gctgcgggct ggctcgccgc aggctggttc
     5281 gctgccgact ttgtggtcgc aggctggtct ttcagccggg cggcggcgct ggagcgctgg
     5341 cgatcccaca ccttggtgat gccctcatcc ctggtgatcg cagcgatgag tttgccatcg
     5401 gcgctgatgt ccacatcggc cacgggcgtg gtgaggccgt aaaaggcgag tcgttcctca
     5461 ccgctggagg catcgtggat gcgcacgctg ccatcgtcac cgccactgag gatttcgcgc
     5521 ccatcggggc tgaactcgag gctgctcacg ccctcccagt gagcgcggaa ggcggcctcc
     5581 aactgacccg tcgccgcttc ccagacacga atccagcggt cgggtccgcc cgtggcgagg
     5641 tgcaggccgt cggggctggc ggcgaagcag gcgatgctgc cggtctgcgc ggcgtcgatc
     5701 tgccgcaggg gcttgcccgc ctgccaatcc cacacggtga tcccaccagt gagcgcggcg
     5761 gtggccacgg cggcatcgtc gaggcaaaag gcagcgaagg gctggccggg cttttcgaaa
     5821 gtgatcttct gcagctcttt gcctgaaggg tattcgacca tactgaaccc gttccccgga
     5881 ttggtgtccg tgaacgcgta tgccaccgtg agaaggagac ggccgctgcg attgaatttc
     5941 aggctgctta cctggttggt gccgaaatcc tccaacctct ttccggcagt tgagacgtgg
     6001 gtgaatgaag ttctgccacc catctcgctg tagaggatgg actcaccagc cgggtggatc
     6061 gccagatcgc ggatcttccc ttttgttgtg cgctgagaat tccacttctg tttgcccgtc
     6121 agtggatccc aggcgccgac gtcccctgct gtggccgcat agaccaagga gccgtctgca
     6181 ttgaacagga cgcggcgtcc ttccaaaaat gcatgcataa tggtgctaac ttcctccctg
     6241 agccgggtcg gccagacata ggctccccca tccgcgaacg accgatgagt cgaaatcagc
     6301 gtcatgccat cggggctgaa ggccagagct aacacttcgt tctggacacc attgaatgaa
     6361 cgcagcaagc gccccgtctt cgtcgcccac aggcggatgc ggccgctgct gacgctgttg
     6421 gtcgccaccg aatcacctgt cgggttcatc tcgatcacgg tctctcccgg gaaatggaga
     6481 gcacggattt tttgagccgt gcgcggattc cacatgatca actcatcgcc gccgaggcag
     6541 gcgaacttgt caccggcctg attgaattgc agggtggttt tggtgggatt atccgcgctc
     6601 gcctcgagtg tgctgcgcac ctcgcccgtg ttcgggtcga gaagttgaac ctgcaattcg
     6661 agcccttttg tggacgcaag atgcgccact gtctgtccat ccgggctgaa ggacgggacg
     6721 acaccgacga agggctgagt ccagagcggc gctcccgtct ccgcagcgta ggcgatgagc
     6781 ttccgttccc cggccacaag caccgtcgcg ccatcgggac tgagcagcac acgctcgatc
     6841 gcgcggatct cgctggtcca gcggtgcagg accgtgcggg tcgccagatc ccacaaggcc
     6901 acttcgttgg cggtcaccgc ttgagcgact ctgccgccct cccgatctat cccgaaggtg
     6961 atggggtagt attcggtctg gagcagaaag tcaccgatct gatgtcccat caggctgaaa
     7021 atgccgacac cagccacttt agacgggccc acccagcacc tggctgctaa acgatcgccc
     7081 ggcggcacaa accgcagccc ataagccagt cctccatcga gaagcggcaa ttgggaggtg
     7141 tagtcggtcg agtgggcctg caggaatcgc caggtcgcat cgcggaagct ctctggcgtc
     7201 tccccgagga tcttctgtgc ctcgacgaat ttgccgcgct cgaattcctt ctccgcaagg
     7261 tcgagctgcg acttcgccag cgccttccgt gccgtttccc gctcttcgac ggcgatgact
     7321 tcggcctgag tcgcacgttg ctccttcgct cgaagattga agatgaacca caccgcgagt
     7381 ccgctgatca gcagccaagc cgccgcggca gtggtgaaga tgcctttgtt gcgcttgatg
     7441 aggagggcga gctgcttcgc cagccctgcc tgttccgctg tggtggcaaa accgccctga
     7501 aatgcctcga tgtccgcgct gaaggccgcc acgtcctggt agcgcttggc tttgtcgagc
     7561 gtgagtgcct tcatcaccac ggcggagagt gcatttggca cacgcccgcc cggcagatgc
     7621 ggcagcggag tgattttctt cgcttcgagc acctggccct tcacggcgga tttgcccttg
     7681 cccgtggtgg tggctccata agtcgtgggc gaggcaatgc gggccgtgct gaccttttcg
     7741 atcacctccc acacgtcctt cccctccacc ggcggtcgca gggtgaggat ggcgtaaaga
     7801 atgccgccga gggaaaagac atccgagcgg gcatccatgt ccgtgacctg ccccagcgcc
     7861 tgctccggac tcatgtattg cggcgtgccc atcaccgcgc cttcgagcgt ttgcgcgggg
     7921 gtggcttcat tcaaggagcg ggacttgcca agtcccgctt cagtgactga cggcacttgg
     7981 caagtgccgc tccttgagtc gtcgaggatc ttcgcaattc cccagtccat gaccagcacc
     8041 tcgccgaact cgcccaccat gatgttttcc ggcttcaagt cgcggtggat gatcttttgc
     8101 gcatgggcaa aggccagcgc gtcgcagact ttgcggaaga tggtgagcag gcggtcgagc
     8161 gtgtaatgct ccaaggcctc tttgtcctcg tggcgcagat catcgatgat gtgctggagc
     8221 gtgcggccct tcaccagctt catcgtgtag taaagagagc cttccgaatc acgcccgagg
     8281 tcgtggatgg gcacgatgtt gggatgctcc agcctgccga gcacggcggc ctcctggatg
     8341 aagcgctgct tttgatcctc gctgcatgcc gcctcggaaa gcatgatctt caccgcgatg
     8401 gtgcggccca gcttgcaatc gctggcctcc aggatcgccc ccatgccacc ctgggcgatg
     8461 cgacggccaa aaatgaaggg cacgggcgag tcaggattcg ccgacgaggc agagacaggc
     8521 ggtggcacga tctgccgcgg cagtgcggcg ggggccagtt ggatcgtggc atcgaaatca
     8581 ggctcttcga gggcctccac gccctttcgc atcacggagg gaggttgcaa aaaggaccgc
     8641 gaatccgcct ccaggcccag cagccgctca acgcgagccc ggagctccgc atcaccagca
     8701 caggcagagt cgagataggc cgcctgagcg gagggatcgt ccacatcgag tgctgccatg
     8761 aagatttctt tctcacggct ggaggaggat ggatcggagg cggacatgag cgtggttctg
     8821 gagatcatgc cccatcggcg ggcgaaagcg gcacaatttt tagcgtaggc cggatgtgtt
     8881 tggcggagaa cgcttcattt cgcctccagc actcttcgcc a
//
LOCUS       DAOMYJ010000208         8919 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Prosthecobacter sp. isolate AMR_MDS_5629
            NODE_2602_length_8919_cov_21.469765, whole genome shotgun sequence.
ACCESSION   DAOMYJ010000208 DAOMYJ010000000
VERSION     DAOMYJ010000208.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867850
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prosthecobacter sp. (wastewater metagenome)
  ORGANISM  Prosthecobacter sp.
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Verrucomicrobiaceae; Prosthecobacter.
REFERENCE   1  (bases 1 to 8919)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8919)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.30
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:34:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,742
            CDSs (total)                      :: 4,684
            Genes (coding)                    :: 4,676
            CDSs (with protein)               :: 4,676
            Genes (RNA)                       :: 58
            tRNAs                             :: 55
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8919
                     /organism="Prosthecobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2602_length_8919_cov_21.469765"
                     /isolate="AMR_MDS_5629"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1965333"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..427)
                     /locus_tag="PLP58_17550"
     CDS             complement(<1..427)
                     /locus_tag="PLP58_17550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ10389.1"
                     /translation="MSIPRLISLSLVFICAIHPVSAHTHREVVLGLLLLLGLLEAGRG
                     VETAGTATEQLRAWLGRLVSLSVWPFHAAVLALAFLGLSAHEGPPAAKIVSSCATCAG
                     AKSSGCGGSGGGGCGSPAGTGSSCGCSSRATASSAPAARA"
     gene            complement(543..1535)
                     /locus_tag="PLP58_17555"
     CDS             complement(543..1535)
                     /locus_tag="PLP58_17555"
                     /inference="COORDINATES: protein motif:HMM:NF025006.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HRJ10390.1"
                     /translation="MKRNLINLGLLLVLVAMWWLLRLEPHEQPSQERLAQKKTSKVPA
                     ALPPLKPEQKQTQTNPEKLAGFKASFATPINLYGKVLDQHGDPVSTASVQYSVLGLTG
                     KQKLQTTSKRDGSFEITGHRGGTLSVEVSKAGYRRLPGADDKVGSGKLFYFGLGRPPA
                     SSPQEPVVFTLQKPGVIEPLIHAKERNYRLSRDGSPMEIDLHPGGSSANRVVLRCWNK
                     ELEPRPQTELRYDWRLEIAVPGGGITERKDIMAFEAPESGYKQKAIIDMPVDAKPHWS
                     ASAERTYFIRFEDNTFARVALRMRARGDHFVVWESHLNPKPGSRNLDTDPGNAD"
     gene            complement(1541..6307)
                     /locus_tag="PLP58_17560"
     CDS             complement(1541..6307)
                     /locus_tag="PLP58_17560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ10391.1"
                     /translation="MLSWTLTSPVATFGGYWAMDGAGQYYWVPDFEGDEPTEPPPGSG
                     GTGPEMFEWHGGVWIIDGVAVEFTGTSTYQDFIPDADGDGLPDDWDPHPYDAANNSHW
                     FGGGTWAIDGVLQSWEGLWLAGTGPLPDDDNDGLPDDWDPHPYDPANNTFEFSGGIFT
                     RHGLTLALRSGFYAGSGVDGDEDGFPDDLDDRFINPASYGALQSWAGGTFLINGAPST
                     YGPLHYFAPAVADTDADGIPDELDAAPEDPWNGTYFHWSGGVFHVDGVPTTFAPRYHG
                     GLFADGDGDTLPDVADPYPADPANNSAWWSGGTFTIDGIPTEFPGQWHRANAGDGDND
                     GIPEDMDPYPGDPNNPTLHQFIWQGGTFIINGQAQTLQPGTHPGVWADTDEVLIPDSL
                     DPWPNDPSNNSAWWQGGTFTVDSQPHTFPAQWHRANAGDADADGIPDDLDPYPNDPLN
                     PTPVTHSWTGGVFRVNGVEQTLPGGTYPGLWSDVDGDTIPDAADPYPADPANNSLWWP
                     GGVWLIEGAQQTLAAQWIAASAPDGDGDGIPDAFDPHPADSWNGTGWWWPEAPSLTVK
                     IHNQNVTFPRQHYLTAWSDSDGDGIPDIADPLPHDYYNGNDTDADGIPDSIEAQYPGL
                     LDLSDPADAQSVRADGVSHLRAHLYNQQAAAASRPGLILPLTTLIPQERDTDLDTMPD
                     RYEAAYGLNPFDNQDAAGNTHDDLVLLAEKAAAGLNPWEEVLPEDYLAVTGQDPSTMS
                     NHDPVKSYAENDWDGDGVSNVDELLVFHTALRNTSSIPDESQILAAMLSGQVSQTTWL
                     NFEHLIQPDTMCSCGGPDCGIHGCTCTVICDNWTPHYCGCGGLSCHVYECHCTEMCGG
                     DPGGGGGGGGGGSGGGGSGGGTGTGPCQCERSQHPAGSQCGCPEGHSCPENELCPPPP
                     GQGTCSTECPASEGNCSCLSGGLGRCTSASLGSLPPNCSTDSTGCECAAGCGATPPDP
                     NVEGDEGTPCPCRISCNPQSFPCVCDCDPEFGGCAASPNPEIDHATCIAGTLTDPPPG
                     HVRTGELPCSHFILVELAVDADRDGLVTFGSDSTSSDKPFRFWVNDDHDHGVDTEDLN
                     GDRNCDDGMLNGIRDLEDFTVLKMRIGALRQTLSQQNGKIGFRWKDISGGSPSCRIFT
                     GSPSLEKAADYLSTADGAETVRAQNLFVMNTLVSGGSAAWLRSSLLTETSSDEITLLM
                     EGCADGKGKLTLVFELKGQMVDGPSLDVQLLRVGEMYDRGRITVDAPGIPNPWDNVNP
                     PALAWAPDTAAGSPKVDPHAKAKTIIWVHGWRMDYAEYASWGDTSFKRLWQMGFTGRF
                     YAFRWPTFSGENDGLPSLENITSYDLWRDGGLTYNASEYRSWLSGPALANFVNQTANS
                     DSRYLFAHSMGNVISGAALRAGMNVTKYAMCNAAMASMAYDGVHNDELDNDFETIDTD
                     PTPLAAAIWGLSNRFNPTGRTTIVNFALPMDDALGLWSFNNKWFKPYNFLVWGYGYDR
                     WNPYPNEKLWYGNLGGISFVRYLTTDAEAYGYCVQARTRTAGAKLGVGNMSSTVDMST
                     FGFGDEHSAEWNRSYQNSYGFWKRLMEEFDEDVSNR"
     gene            6546..7004
                     /gene="moaC"
                     /locus_tag="PLP58_17565"
     CDS             6546..7004
                     /gene="moaC"
                     /locus_tag="PLP58_17565"
                     /EC_number="4.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007414945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic pyranopterin monophosphate synthase MoaC"
                     /protein_id="HRJ10392.1"
                     /translation="MPDLTHLDSTGSASMVDVSAKPLMTREAVATGFIHLKPETLSLI
                     RAQALKKGDVLATARIAGIQAAKQTQLLIPLCHQIPLSKVAVSFDERATGIAVTAGVK
                     TVAPTGVEMEALTAVSIAALTIYDMCKAVDKEMRITDIHLISKTKIPSVA"
     gene            7044..7817
                     /gene="hisA"
                     /locus_tag="PLP58_17570"
     CDS             7044..7817
                     /gene="hisA"
                     /locus_tag="PLP58_17570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009963510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003949 -
                     1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformimino-5-aminoimidazole
                     carboxamide ribotide isomerase"
                     /protein_id="HRJ10393.1"
                     /translation="MTRFRPCIDLHDGQVKQIIGSTLKDEGAGPKVNFVSDRSPAWFA
                     ELYRRDGLTGGHVILLGKGNEQAAREALAAWPGGMQVGGGIRPCNAQEHLAAGASHVI
                     VTSYLFETDGTFSETRLEKMTRAAGKERLVIDLSCKATPEGWTVAMNRWQTLTDLSIT
                     PATLIHLAGHCAEFLIHAVDVEGKCEGMDEDLVRFLAEHSPIPATYAGGIRHLDDLRR
                     LDELSSGRLDGTIGSALDLFGGTGARYEDCVAFNHRSVQ"
     gene            7836..8639
                     /locus_tag="PLP58_17575"
     CDS             7836..8639
                     /locus_tag="PLP58_17575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009962730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HRJ10394.1"
                     /translation="MKIIRHLDSEGKTAYAALQPDGRALVIEGCIFSEHTVTDREAVI
                     SRHLAPIKPRAILCIGLNYKFHAEESGAAIPQNPVLFMKLPGTVQNPGEAILLPTHLK
                     SEKVDYECELAVVIGKAAKNVSKEDALDYVLGYTCANDVSARDWQLNGGGNQWCRGKT
                     FDTFCPLGPVLVTRDEIPNPNALGIKTVLNGETVQDWNTNDMIFDVPTLISFLSGSTT
                     LEPGTVILTGTPHGVGMARKPPLWLKDGDSVTIEIEGIGALTNPVRDEA"
     gene            8652..>8919
                     /locus_tag="PLP58_17580"
     CDS             8652..>8919
                     /locus_tag="PLP58_17580"
                     /inference="COORDINATES: protein motif:HMM:NF014165.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase M32"
                     /protein_id="HRJ10395.1"
                     /translation="MPVSAYDQLVSLAREIAILSSTEALISWDQETYMPPGALDHRAR
                     QLSYLNGKAHALATGPSFRKLLDKAEAAPPPDAKAAANLREMRRA"
BASE COUNT         1710 a         2699 c         2790 g         1720 t
ORIGIN      
        1 ctgcgcgtgc tgcgggcgcg cttgaggctg tggcgcggct ggagcagccg cagcttgagc
       61 cggtgcccgc cggggaaccg cagcccccgc ccccggagcc gccgcagccg gagcttttgg
      121 ctcccgcgca ggtggcgcaa ctggagacga tcttcgccgc cggcggtccc tcatgcgcgc
      181 tcaaccccag aaacgccagc gccagcaccg ccgcatgaaa cggccacacc gaaagcgaaa
      241 ccagcctgcc cagccaagcc cgcaactgct ccgtcgccgt accggcagtc tccacacccc
      301 tccccgcctc aagcaacccg agcaggagca gcaaaccgag aaccacctcc cggtgcgtgt
      361 gcgcggagac tggatggatg gcgcagatga agacgagaga aagagagatg aggcggggaa
      421 tggacatcga gggcttctag ttggcagatg ctgagatgat taacgatttc gaaaaatgac
      481 tggctacctc actgtctttt cctcgacgct acggagctgg tgagatttgc gatgctctga
      541 gattagtcag cgttacccgg atcagtgtcg aggtttcgtg agcctggctt cgggttcaaa
      601 tgcgattccc aaacaacaaa gtgatcgcct cgtgcgcgca ttcgcaatgc aactcttgca
      661 aacgtgttgt cctcaaagcg aataaaatag gttcgttctg cgctcgcact ccaatggggt
      721 ttggcatcca ctggcatatc aatgatagcc ttttgtttgt agcccgattc tggagcttca
      781 aaggccatga tgtcctttcg ttccgtgatg ccgccaccgg gaacagcaat ctccagcctc
      841 caatcgtatc gcagctcggt ctgaggtctc ggttcgagtt ctttgttcca gcatcgaagc
      901 acgacccgat tggcgctgct gccacctgga tgcaaatcga tctccattgg gcttccatca
      961 cgactcaggc gatagttgcg ttcctttgca tgaatgagcg gctcaattac tcctggcttt
     1021 tgaagtgtga acacaaccgg ctcttgcggg ctggatgcag gcggtcgtcc aaggccaaaa
     1081 taaaagagct ttcctgaccc aaccttgtcg tcggctcccg gcagtctgcg gtagcctgcc
     1141 ttgctcactt caacggagag agtcccgcca cgatgaccgg tgatctcgaa acttccgtcg
     1201 cgcttgcttg tggtttgcag cttttgcttt ccagtgagtc caagcacgga gtattgcaca
     1261 ctcgctgtag acacgggatc gccatgctga tctaagacct tgccataaag gttgattggt
     1321 gtggcgaagg atgctttgaa acctgcaagc ttttcaggat tcgtctgtgt ctgcttctgt
     1381 tctggcttta gtggtggtag tgccgcagga acctttgacg tttttttttg cgcaagcctt
     1441 tcttgtgatg gctgctcatg cggctctagg cgcaaaagcc accacatggc taccagcacg
     1501 agaagaagtc ccaagttgat gagatttcgt ttcatgcgtt ttaacgattg gatacgtctt
     1561 catcaaactc ttccattagc cttttccaga acccgtatga gttctgatag gagcgattcc
     1621 attccgcact gtgctcgtct ccaaatccaa aagtgctcat gtccactgtg gaactcatgt
     1681 tgcccacgcc gagtttcgcg cccgctgtgc gagttcttgc ttgcacacag tatccgtagg
     1741 cttccgcatc ggttgtcagg tagcgaacga aagagatgcc gccgaggttg ccataccaaa
     1801 gtttttcatt cggatatgga ttccaacggt cgtaaccata gccccagacc aaaaaattgt
     1861 atggcttgaa ccacttgttg ttaaaactcc acaatccaag cgcatcatcc ataggcaacg
     1921 cgaagttcac gattgtcgtt cttcctgtag gattgaacct gttggacaag ccccaaatgg
     1981 cggcagcaag cggcgtcgga tcagtatcga ttgtttcaaa gtcgttatcc agttcgtcat
     2041 tgtgaacacc atcgtaagcc attgaggcca tcgccgcgtt gcacatggca tacttcgtca
     2101 cgttcatgcc ggctcggaga gccgctccag agatcacgtt gcccattgag tgggcaaaca
     2161 agtaacggct gtcactgttt gcagtctgat tcacgaagtt cgccaaggcc ggacctgaaa
     2221 gccaggaacg atactcactc gcgttgtagg ttaagccgcc gtcacgccaa aggtcgtagg
     2281 aagttatgtt ctcgagactt ggcaagccat cattctcgcc gctgaatgtg ggccaacgga
     2341 acgcatagaa ccgccctgtg aagcccatct gccagagccg tttgaagctg gtgtcgcccc
     2401 atgaggcata ttctgcgtaa tccatgcgcc agccgtgaac ccaaatgatg gtcttggctt
     2461 tggcatgggg gtcaactttt ggtgatcctg ctgctgtgtc cggtgcccag gccagtgcag
     2521 gtggattcac gttatcccat ggattgggga ttccaggtgc atctaccgtg atacgcccac
     2581 ggtcatacat ctctccgact cgaagaagct gaacatccag acttgggccg tcaaccattt
     2641 ggcccttcag ctcaaaaacc aaggtgagtt tgcccttgcc gtccgcgcag ccctccatga
     2701 ggagcgtgat ctcatcggat gacgtttcag tcagaagaga agaacgaagc caagctgcgc
     2761 ttcctccaga aacaagagtg ttcatcacga acaggttctg tgcacgaacg gtttcggctc
     2821 catcagcagt ggacagataa tcggcagctt tctccagtga aggcgagccg gtgaagattc
     2881 tgcacgatgg agagccgcca gagatgtcct tccatcggaa tccgatcttt ccattctgct
     2941 gggacaacgt ttggcgcagt gcgccgatac gcattttcag gacggtgaaa tcctccaggt
     3001 cacggatgcc gttgagcatt ccatcatcgc agttgcgatc accgttgagg tcttcggtat
     3061 caaccccatg atcgtgatca tcattgaccc aaaagcggaa aggtttgtcg ctcgaagtgc
     3121 tgtctgagcc gaatgtgaca agtccatcgc ggtcagcatc aactgctaat tccacaagta
     3181 taaagtgcga gcacggcagc tcccctgtgc gcacatggcc tggaggaggg tcggtgaggg
     3241 tgccggcgat gcaggtggcg tggtctattt ccgggttggg ggaggcggcg cagccgccga
     3301 actcggggtc gcagtcgcag acgcagggga aggactgcgg attgcaactg atgcggcagg
     3361 gacagggggt gccttcgtct ccctccacat tggggtcggg cggggtggcg ccgcagccgg
     3421 ctgcgcattc gcagcccgtg ctgtccgtgc tgcaattcgg cggcaggctg ccgagtgagg
     3481 cgcttgtaca gcggccaagg ccgcctgaca aacaggagca gttgccctcg ctggcggggc
     3541 attcggtgga gcatgtgccc tggccggggg gaggagggca gagttcgttt tcagggcatg
     3601 agtgcccctc cgggcagccg cactggctgc cagcagggtg ctgggaacgc tcgcactggc
     3661 acgggccggt gccagtgccg cctcctgagc caccaccccc acttccgccg ccgcctccgc
     3721 cgcctccccc tccagggtcg ccgccgcaca tttcggtgca gtggcattca tagacgtggc
     3781 aggagagacc gccgcagccg cagtagtgcg gggtccagtt gtcgcagatg acggtgcagg
     3841 tgcagccgtg gatgccgcag tcggggccgc cgcaggagca catggtgtcg ggctggatga
     3901 ggtgctcgaa gtttagccag gtggtctggg agacctggcc ggagagcatg gcggcgagga
     3961 tctggctttc atccgggatg gaggaggtgt tgcgcagggc ggtgtggaag acgagcagtt
     4021 cgtccacgtt actgacgccg tcgccgtccc agtcgttctc ggcgtagctt ttcactgggt
     4081 cgtggttgct catggtggag gggtcctggc cggtgacagc caggtagtcc tccggcagga
     4141 cctcctccca ggggttgagt ccggcggcgg ctttttcggc caggagcacg aggtcgtcat
     4201 gggtgttgcc ggcggcgtcc tggttgtcga aggggttcaa accgtacgcg gcctcgtagc
     4261 ggtcgggcat ggtgtcgaga tcggtgtcgc gctcctgggg gatgagggtg gtgaggggca
     4321 ggatgaggcc gggcctgctg gcggcggcgg cctgctggtt gtagagatgg gcgcgcaggt
     4381 gggagacgcc gtcggcgcgg acgctttgcg cgtcggcggg gtcgctcaaa tcgagcaggc
     4441 cggggtattg ggcctcgatg ctgtcgggga tgccgtcggc gtcggtgtcg ttgccattgt
     4501 aatagtcgtg cggcagcggg tcggcgatgt cggggatgcc gtcgccgtca ctgtcactcc
     4561 aggcggtgag gtagtgctgg cgggggaagg tgacgttttg gttgtggatc ttgacggtga
     4621 gggagggcgc ctcgggccac caccagccgg tgccgttcca ggagtcggcg gggtggggat
     4681 caaacgcgtc gggaatgccg tcgccatcgc cgtccggggc gctggcggcg atccactggg
     4741 cggcgagggt ttgctgcgct ccttcgatga gccagacgcc gccgggccac cagagcgagt
     4801 tgttggcggg gtccgctgga taagggtcgg cggcgtcggg gatggtgtcg ccgtccacat
     4861 cgctccagag gccggggtaa gtgccgccag ggagagtctg ctccacgccg ttgacgcgga
     4921 aaacgccgcc cgtccaggag tgggtgacgg gtgtggggtt gagcggatca ttgggatagg
     4981 gatcaaggtc gtcggggatg ccgtccgcat cggcatcgcc cgcattggcg cggtgccact
     5041 gggcggggaa ggtgtgcggc tggctgtcca cggtgaaggt gccgccctgc caccaggcgg
     5101 agttgttgga ggggtcgttg ggccaggggt caaggctgtc ggggatgagg acctcatccg
     5161 tgtcggccca gacgcccggg tgggtgccgg gctgtaaggt ctgcgcctgg ccgttgatga
     5221 tgaaggtgcc gccctgccag atgaactggt ggagggtggg gttgttcgga tcacccggat
     5281 aagggtccat gtcctcggga atgccgtcat tgtcgccatc gccggcattg gcgcggtgcc
     5341 actggccggg gaactcggtg gggatgccgt ctatggtgaa ggtgccgccg gaccaccagg
     5401 cggagttgtt ggcgggatcg gcgggatagg ggtcggcgac atccgggagg gtgtcgccat
     5461 cgccgtcggc aaagaggccg ccatgatagc ggggcgcgaa ggtggtgggc acgccgtcca
     5521 catggaagac accgccgctc cagtgaaaat aggtgccgtt ccacggatcc tcgggcgcgg
     5581 cgtccagctc gtcgggaatg ccgtcggcgt cggtgtccgc gacagcgggg gcgaagtagt
     5641 ggagaggacc ataggtgctg ggcgcgccat tgatgaggaa ggtgccgccc gcccaagact
     5701 ggagcgcgcc gtaggaggcg gggttgatga agcggtcgtc gaggtcatcg ggaaaaccgt
     5761 cctcatcccc gtccaccccg ctgcccgcgt aaaagcccga gcgcagggcc agggtgagac
     5821 cgtgacgggt gaagatgccg ccggagaatt cgaaggtgtt gttggcggga tcgtacgggt
     5881 gcggatccca gtcatcgggc aggccgtcat tgtcatcatc aggcagagga cccgtcccgg
     5941 ccagccacag gccctcccag ctctgaagca cgccatcaat ggcccaggtg ccgccgccga
     6001 accagtggct gttgttggcg gcgtcgtagg gatgggggtc ccaatcatcg ggcaggccgt
     6061 cgccatccgc gtccgggatg aagtcctgat aagtggaggt gccggtaaac tcgacggcca
     6121 cgccgtcaat gatccagacg ccgccatgcc actcgaacat ttcggggccg gtgccgccgg
     6181 agccgggcgg cggctcggtg ggttcgtcac cttcgaaatc cggcacccag taatattggc
     6241 ccgcgccatc catcgcccaa taaccgccaa aggtggccac cggggaggtc agcgtccagg
     6301 agagcatgat gaggaactgc aaccagccga accagcgttt acgaccggaa taaagcttcg
     6361 agcttcgcgg ttcgcggttc gcggttcagg cgggaggtga gagttcatgc ggaaagctgc
     6421 gtttggaatt tccggattat ttcctgacac cagccgttaa accaaaaacg cgggactttg
     6481 acaagaaaaa tgtcggggga aagattctgg gatttcggat tccgaatttc gagcttacct
     6541 gccccatgcc cgatctcacc cacctcgatt ccaccggcag tgcaagcatg gtggatgttt
     6601 ccgccaagcc actcatgaca cgcgaggctg tggccactgg attcatccat ctcaagccgg
     6661 aaacgctgag tctgatccgc gcgcaggctt tgaaaaaagg cgacgtgctt gccactgcgc
     6721 gcatcgctgg catccaggcg gcaaagcaga cgcagttgct gattccgctg tgccaccaga
     6781 ttcccttgtc aaaggtggcc gtgagcttcg acgagcgcgc gactggcatc gccgtcaccg
     6841 ccggggttaa aactgtcgcg cccacgggtg tcgaaatgga ggcgctgacc gccgtgtcca
     6901 tcgccgcgct gacgatttac gatatgtgca aggccgtgga caaagaaatg cgcatcacag
     6961 acatccacct catctcaaaa accaaaatcc cctccgtcgc ttgacccgcg cgccgcgcgc
     7021 cgccatgtca gccgccagca cgcatgaccc gtttccgccc ctgcattgac cttcatgatg
     7081 gccaggtcaa acaaattatc ggctccaccc tcaaggacga gggcgcaggg ccgaaggtca
     7141 attttgtcag cgaccgctcc cccgcctggt tcgcggaact ttaccgtcgc gacggtctta
     7201 ccggcggcca cgtcatcctg ctaggcaaag gcaacgagca ggccgctcgc gaggccctag
     7261 ccgcctggcc gggcgggatg caagtgggcg gcggcatccg cccgtgcaat gcgcaggaac
     7321 atctcgccgc tggcgccagc cacgtcatcg tcaccagcta cctctttgag accgacggca
     7381 ccttttccga gacaagactg gagaaaatga cgcgcgcggc aggcaaagag cggctggtca
     7441 ttgacctgag ctgcaaagcc acccccgaag gctggaccgt ggccatgaac cgctggcaga
     7501 cgctcaccga cctgagcatc acacccgcca cactcatcca cctcgccgga cactgcgccg
     7561 agtttttgat ccatgccgtg gatgtcgagg gcaaatgcga aggcatggac gaggacctcg
     7621 tgcgctttct ggccgagcac agccccatcc ccgccaccta cgccggcggc atccgccatc
     7681 tcgacgatct gcgccgtctg gatgaactca gctccggccg gctagacggc accatcggca
     7741 gcgccctcga tctctttggc ggaaccggcg cccgctatga agactgtgtt gccttcaacc
     7801 accgcagcgt gcaataacac gcccctctcc ccgccatgaa aatcatccgc catctcgact
     7861 ccgaaggaaa aaccgcctat gccgccctcc agcccgacgg acgcgcgctc gtcattgaag
     7921 gctgcatctt cagcgagcac accgtgaccg accgcgaggc cgtcatctcc aggcacctcg
     7981 cccccatcaa gccgcgcgcc atcctctgca tcggcctcaa ctacaaattc cacgccgagg
     8041 aaagcggcgc cgccatcccg cagaaccccg tgcttttcat gaagctgccc ggcaccgtgc
     8101 agaaccccgg cgaggccatc ctgctgccca cccacctgaa gagtgaaaag gtggactatg
     8161 agtgcgagct ggccgtcgtc atcggcaagg ccgccaaaaa cgtctccaag gaggacgcgc
     8221 tggattatgt gctcggctac acctgcgcca acgatgtcag tgcgcgggac tggcagctca
     8281 acggcggcgg caaccaatgg tgccgcggca agaccttcga caccttctgc ccgctcggcc
     8341 ccgtgctcgt cacgcgcgac gagattccga acccgaacgc cctcggcatc aagaccgtgc
     8401 tcaatgggga gaccgtgcag gactggaaca cgaacgacat gatcttcgac gtgcccacgc
     8461 tgatctcctt cctcagcggc agcaccacgc tcgagcccgg aaccgtcatc ctcaccggca
     8521 cgccgcacgg cgtcggcatg gcgcggaagc ccccgctctg gctcaaggac ggcgacagcg
     8581 tcaccatcga gatcgaaggc atcggcgctc tcaccaatcc cgtgcgtgac gaagcttaac
     8641 cgcccctgtt catgcccgtc tccgcttacg accagctcgt ttcactcgcc cgtgagatcg
     8701 ccattctcag ctccactgag gcgctcattt cctgggacca ggaaacctac atgccgcccg
     8761 gcgcgctcga ccaccgggcg cgccagctct cctacctgaa cggcaaagcg cacgccctcg
     8821 ccaccggccc cagcttccgc aaactcctcg acaaagccga ggccgccccg ccaccggatg
     8881 ccaaagccgc cgccaacctg cgcgagatgc gccgcgccc
//
LOCUS       DAOMYJ010000209         8909 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Prosthecobacter sp. isolate AMR_MDS_5629
            NODE_2606_length_8909_cov_20.593178, whole genome shotgun sequence.
ACCESSION   DAOMYJ010000209 DAOMYJ010000000
VERSION     DAOMYJ010000209.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867850
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prosthecobacter sp. (wastewater metagenome)
  ORGANISM  Prosthecobacter sp.
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Verrucomicrobiaceae; Prosthecobacter.
REFERENCE   1  (bases 1 to 8909)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8909)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.30
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:34:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,742
            CDSs (total)                      :: 4,684
            Genes (coding)                    :: 4,676
            CDSs (with protein)               :: 4,676
            Genes (RNA)                       :: 58
            tRNAs                             :: 55
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8909
                     /organism="Prosthecobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2606_length_8909_cov_20.593178"
                     /isolate="AMR_MDS_5629"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1965333"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1089)
                     /locus_tag="PLP58_17585"
     CDS             complement(<1..1089)
                     /locus_tag="PLP58_17585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009966068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ10396.1"
                     /translation="MSTVQTTAAGRKFPKFDLYRLLHTVFEPVFGRKICILIDLPELA
                     EAKDMAFLKNPARAIQRRAHDYFYKGLHGGVMDELALSGGEMFAYLQTGGSNLDLPDE
                     CADMQGGRLSLERDVYPNYDIILCISTFSATAPLTAFARQYGFRGATLHGLNDIILNS
                     GLAVDYNIVSRDAEKLRQAMTRADRVEIDFALEDGEVLTARLELGGQEAQKSHGLCRG
                     DTPDVANLPAGEVYFVPKSAEGQFPMKYEDGTLGRLIVTGGRIIRSELIAGDPAVIDA
                     HNARLQDDPMTGVLGELGFGTQVLPVSGADIQDEKVLGTCHLATGRDDHLGGHITPDL
                     FKRRQNATHDDILFAPHKTPNFDIKQVRM"
     gene            1306..3249
                     /locus_tag="PLP58_17590"
     CDS             1306..3249
                     /locus_tag="PLP58_17590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="VCBS repeat-containing protein"
                     /protein_id="HRJ10397.1"
                     /translation="MSSSFKRSAAALLILFACQCGAQPHLPQGTKALPSAAEKGRGPL
                     ALVNLHHHVLGHARVFGGKMPDLFVAGYGGPQAVHLFRWVDTAENGAPVFARPVEVRC
                     DFKDKGGIFQNPDGAITGLWISKEHLVTTEFDRSALAFRETQRVRLPAEIKSPASLGV
                     ILNSDGTHDLAFELSNGAKGTPGHPWTEEWRPYNSSGIATGGLRYRYLMGWSADGQVK
                     QITATKNEVYFSMHDLTAVDLGPGHERDLITGSRQGNLVHYRNKAGRGFDLEKKRLVA
                     GPDGNALRHPSINPSVCAYPGGLIACGEGSLYFYRFTGEWAATGAPVFSGPVSVLQEN
                     ADLYAGTLPSPTTVDWDGDGALDILAGNSEGFVLFFKNTGGNESPRFLPATRVQAGGR
                     DIHVQAGYSGSLQGLQEARWGYLSPNAFDWNGDGLPDLVTGDITGDYHVYLNRGTRTE
                     PRLDPARPLYCDGINLHGMWRCRPALARIGGRAALVIVDGEDHFHLYWRMDDFNVEDG
                     GKLKLEDGKPIGASGGVGGKSGRAKPDLFDWNQDGHLDLVIGTNRVNSIPDRATGLPM
                     PGIGIKTLGTPLLMLNTGTNAKMKFARPVPFRDTSGVIIQPGGAHETGAVGTMLGGDG
                     PNLLVCNEAGRMFLLPRKSLLGW"
     gene            3313..4299
                     /locus_tag="PLP58_17595"
     CDS             3313..4299
                     /locus_tag="PLP58_17595"
                     /inference="COORDINATES: protein motif:HMM:NF019474.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HRJ10398.1"
                     /translation="MKPIFLLLALCATAVFSQTPKQDPKPYQLPAGIKMERDIAYIEG
                     GDEAQKLDIYLPETPDGKPLPLIVHVHGGGWRGGNKFPCPVAGMALKGYAVASVEYRF
                     SQKAVFPAQIQDCQAAIRWLRAHAERFNLDTEHVGVIGGSAGGHLSALAGTSGGKKAF
                     PPIGGHLDQPDRVQAVCDIFGPADFTTVMRHAEEDKNVRNVFAFNTPGDPYSGLIGTS
                     LDDKSKAGAASPAHYVSKDSPPFLILHGTHDALVPYAQSVQLEAALKAQGVPVWLQTL
                     PGSGHGGPAFVKPSVLELTQNFFDRHLKGADVEIKLVPEDELAVEPPKPAVK"
     gene            complement(4354..5373)
                     /locus_tag="PLP58_17600"
     CDS             complement(4354..5373)
                     /locus_tag="PLP58_17600"
                     /inference="COORDINATES: protein motif:HMM:NF012640.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate-binding protein"
                     /protein_id="HRJ10399.1"
                     /translation="MTELALFRLPGGRAWLGAGPFEALAEPPSAGGCFYVNDFDLRDP
                     LPWKRPARLVEITPATLRSQAGWNGAEPPRIIWAKPVTEWFKMAFRRIRREVLGRRLE
                     KMVPVLTERGTITAGHPVRLLERVMEAPSNTWGYAFIGQESGFLGASPELLFLMRGAQ
                     IETMALAGTAKPGDRADFLSDVKEIEEHEIVVRYLLERLGMLGDVVREPRGLCEASGL
                     LHFQSRLTLALKSPSSADALIRHLHPTPAVGCLPRDEHWLARLRDYRQQLQVPGFFGA
                     PFGFIEPGADSPCHVIVAIRGIGWQGSEAQLPSGCGIVSGSAFDHEWRELRLKRESVT
                     GMLGV"
     gene            5539..6804
                     /locus_tag="PLP58_17605"
     CDS             5539..6804
                     /locus_tag="PLP58_17605"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF016145.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide pyruvyl transferase family
                     protein"
                     /protein_id="HRJ10400.1"
                     /translation="MRNRRQFISAVTAALGGSVFAADGRPKTLVLQSAWDTVNIGDIG
                     HTPGTLRIIGEHLPGLKVVLWAMKLDERVTAMLKARFPDVEIVQGSLVKETPESARLH
                     EAVRTCDLFIRNSGMGQDISFMQFCRKHGKPYGLFGQSYFPTMVSGEGAEERLDLLNS
                     AAFIYTRETRTLGILKKGGVKCPVMEFGPDGCFGIDVRDDTRGLETMRKLGLEERKFI
                     TVQIRTNTPKYPGVDDPRPEKLNPLHPTPRQTADDERRAAKYRELVTRWVRETGHKVL
                     IAPETIKEMGHNKRLIHDPLPPEIQKHVVNLEYFWNADEAAAIFARACVIICHEPHSP
                     IIGLANGTPIIHTYSEFHSPKCWMFQDIGLPEWLLEMDETPVEKMAETLFAIHADPGA
                     ARAKVERAMSFVHECFGKSMRHVRGVLAA"
     gene            complement(7127..7930)
                     /locus_tag="PLP58_17610"
     CDS             complement(7127..7930)
                     /locus_tag="PLP58_17610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009961546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HRJ10401.1"
                     /translation="MPHVTTNGIQMYYEERGSGEPLVCIMGVTAPGAVWEAHAAEWSK
                     HFRCILGDNRGVGQTDKPEGPYTTAMMADDYAGLMDALGISQARVVGCSLGSVIAQQL
                     ALRHPQKVKSMILMCTWARQDRFGLYTWQHLMKCKASMRPEDFMHYVQMLIFTKPWFD
                     NDDCWENMQQGLKDAATNPAPQPVHAMEAQSAAAITHNTLGELKNVKCPALVIGGKDD
                     AFTPKWMSEEVAAAIPGADLHLYDNAGHAFHWECLSDFNPRTTDWLLKH"
     gene            complement(8062..>8909)
                     /locus_tag="PLP58_17615"
     CDS             complement(8062..>8909)
                     /locus_tag="PLP58_17615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ10402.1"
                     /translation="LPLDEAPAAFSFALAAARIERGSFFMNARDTTVHITGLDYSISG
                     LDMDTLKPGDELDTSIKAHVAVTGMTRIQGVKRKAEIALLDLTGEGRITPLHPQTREW
                     KPSALLTLTLAKGSILGGQQTIGDAAGKDLRKLEEFGIDLAPVPIGGPLQQDAPIQAR
                     WVNGALILPQGCLFVFPDYEVALAPKSWLDTGRDTHELDIRLSCGPELQERLHTGIAG
                     ARLGRDFARGLIAALSDKRGRLTFDIKSRGSLSDPKVTPDTDRALKNLMSGQGLGDLL
                     KGFLK"
BASE COUNT         1545 a         2759 c         2987 g         1618 t
ORIGIN      
        1 catgcgcacc tgcttgatgt caaaattcgg cgtcttgtgc ggggcgaaga ggatgtcgtc
       61 gtgggtggcg ttctggcggc gcttgaagag gtcgggcgtg atgtggccgc cgagatggtc
      121 gtcgcgcccg gtggcgaggt ggcaggtgcc gagcactttt tcatcctgga tgtcggcgcc
      181 tgagacgggc aggacttggg tgccgaagcc gagctcgccg aggacgccgg tcatcgggtc
      241 gtcctggagg cgggcgttgt gcgcgtcaat gacggccggg tcgcccgcga tcaactcgct
      301 gcggatgatg cgcccgccgg tgacgatgag cctgcccagg gttccgtcct cgtatttcat
      361 ggggaactgc ccctcggcgc ttttgggcac gaagtacacc tctccagcgg gcaggttcgc
      421 cacatcggga gtgtcgcccc ggcagaggcc gtggcttttt tgtgcctcct ggccgccgag
      481 ttccagccgg gcggtgagca cctcgccatc ctcaagggcg aagtcaattt cgacgcggtc
      541 ggcgcgggtc atggcctggc ggagtttttc cgcgtcgcgg ctgacgatgt tgtaatccac
      601 ggccaggccg ctgttgagga tgatgtcgtt cagcccgtgc agcgtggctc cgcggaagcc
      661 gtattgtctg gcaaaggcgg tcagcggcgc ggtggcggag aaggtggaga tgcagagaat
      721 gatgtcgtag ttcggataga catcgcgctc caggctgagg cggccgcctt gcatgtcggc
      781 gcactcgtcg ggcaggtcga gattgctgcc gccggtttgc aggtaggcga acatctcgcc
      841 gcctgagagc gccagctcgt ccatgacacc gccatgcagg cctttgtaaa agtagtcgtg
      901 ggcgcggcgc tggatggcgc gggcggggtt tttcaggaaa gccatgtctt tggcctcggc
      961 gagctccggc aggtcaatga ggatgcagat tttgcgcccg aaaaccggct cgaacacggt
     1021 gtgcaggagg cggtagagat cgaacttcgg gaacttgcgg ccggcggccg tggtctgaac
     1081 ggtggacatg gtggaggtca tcataaagag ggggtgcgcc aagggcaaag tcgaatacgg
     1141 cgcgccgcca ttttgcggcg cttcttttgc cgggaaatcc gccgcggcat gagcgcggct
     1201 caggccaaaa atcgtcaacc cgcagcatga atgggcatcc atcacagctt gtgagtccgc
     1261 gcgcgcggct tccgttcact ccgcctgccc aggctgtccc gaaccatgtc ctcctcattc
     1321 aagcgttccg ccgccgcgtt gttgatcctt ttcgcctgcc aatgcggggc gcagcctcat
     1381 ctgccgcagg gaacgaaggc gctgccgtcc gccgcggaga aggggcgcgg tccgctggcc
     1441 ctggtcaatc tgcatcatca tgtgctcggc cacgccagag tcttcggcgg caaaatgccc
     1501 gacctttttg ttgccggtta cggcggccct caggcggtgc atcttttccg ctgggtggac
     1561 acggcggaaa atggcgcgcc cgtctttgcc cggcccgtcg aggtgaggtg cgacttcaaa
     1621 gacaagggcg gcatcttcca aaatcccgac ggcgccatca ccggcctctg gatcagcaag
     1681 gagcatctcg tcaccaccga gttcgaccgc tccgcgctgg cctttcgcga gacccaacgt
     1741 gtcaggctgc cggcggaaat caaatccccc gccagcctcg gcgtcatcct caacagcgac
     1801 ggcacgcacg acctcgcctt cgaactctcc aacggcgcca aaggcacgcc cggccatcca
     1861 tggaccgagg aatggcggcc ctacaactcc tccggcatcg ccaccggcgg gctgcgctac
     1921 cgctacctca tgggctggag cgccgacggc caggtcaagc aaatcaccgc caccaaaaac
     1981 gaggtctatt tttccatgca cgacctcacc gccgtggacc tcggccccgg ccacgagcgc
     2041 gacctcatca ccggctcgcg ccagggcaat ctcgtgcatt accgcaacaa ggcggggcgc
     2101 ggctttgacc ttgaaaagaa gcgcctcgtc gccgggcccg atggcaacgc gctgcgccat
     2161 cccagcatca accccagcgt gtgcgcctac cccggcggcc tcatcgcctg cggcgagggg
     2221 tcgctgtatt tttaccgctt caccggtgaa tgggcggcga caggagcgcc ggttttttcc
     2281 gggccggttt ccgtgctcca ggaaaacgcc gatctctacg cgggcaccct gccttcgcca
     2341 accaccgtgg attgggatgg cgacggcgcg ctcgacatcc tcgcgggcaa ctccgagggt
     2401 ttcgtgctct ttttcaaaaa caccggcggc aacgaatcgc cgcgcttttt gcccgccacg
     2461 cgcgtccagg ccggcgggcg cgacattcat gtccaggccg gctactccgg cagcctccag
     2521 ggcctgcagg aggcgcgctg gggctacctt tccccgaacg ccttcgactg gaatggcgac
     2581 ggtcttcccg acctcgtcac cggcgacatc accggcgact accacgtcta tttgaatcgc
     2641 ggcaccagga ccgaacccag gctcgacccc gcccggccgc tctactgcga cggcatcaat
     2701 ctccacggca tgtggcgctg ccgcccggcc ctggcccgca tcggcggccg ggccgctctc
     2761 gtgatcgtgg atggcgagga ccatttccat ctttactggc ggatggacga cttcaatgtc
     2821 gaggacggcg gcaagttgaa actcgaggat ggcaaaccca tcggcgccag tggcggagtc
     2881 ggcggcaaga gcgggcgggc gaagccggac ctgttcgact ggaaccagga cggccatctc
     2941 gatctcgtca tcggcaccaa ccgtgtcaac tccatccccg accgcgccac cggcctgccc
     3001 atgcccggca tcggcatcaa aacgctcggc acccctttgc tcatgctcaa caccggcacg
     3061 aacgcgaaga tgaagttcgc ccgccccgtt cccttcagag acacctccgg tgtcatcatc
     3121 cagcccggcg gcgcgcatga gacaggcgct gtcggcacga tgctcggcgg ggacgggcca
     3181 aacctgctgg tctgcaacga ggccgggcgc atgtttctgc tgcctcgcaa gtcactcctc
     3241 ggctggtgag cgcggcgtgg agtcattcaa agcatgctcg aaatcgcttc actccgcgtt
     3301 cttgttcccg tcatgaaacc gatcttcctg cttctcgccc tctgcgccac ggcggtcttt
     3361 tcacaaacgc caaagcaaga ccccaagcct tatcaactgc ccgccggcat caagatggag
     3421 agggacatcg cctacatcga gggcggtgat gaggcgcaga agctcgacat ctatttgccg
     3481 gagacaccgg acgggaaacc gctgccgctc atcgtgcatg tccacggcgg cggatggcgt
     3541 ggcggaaaca agtttccatg tccggtggcg ggcatggcgc tcaaaggtta tgccgtcgcc
     3601 agcgtggaat atcgtttcag ccagaaggcg gtctttcccg cgcagatcca ggattgccag
     3661 gccgccattc gctggctgcg ggctcatgcg gagcggttca acctcgacac ggagcatgtc
     3721 ggcgttattg gcggctccgc aggcgggcac ctttccgcgc tcgcgggcac atccggcggc
     3781 aaaaaggcat ttccacccat cggcggccat ttggatcagc cggatcgtgt ccaggccgtg
     3841 tgcgacatct tcggccccgc ggatttcacc acggtcatgc ggcacgcgga ggaggacaaa
     3901 aacgtgagga acgttttcgc cttcaacacg cccggcgacc cgtattccgg cctcatcggc
     3961 acatcgcttg atgacaaatc aaaggccggt gctgcgagcc ccgctcacta tgtcagcaaa
     4021 gacagcccgc cttttctcat cctgcatggc acccacgacg cgctcgtgcc gtatgcgcaa
     4081 agcgtgcagc tcgaagcggc attgaaagct caaggcgtcc ccgtctggct gcaaaccctc
     4141 cccggctccg gccacggcgg tcccgctttc gtcaagccgt ccgtgcttga gctcacgcag
     4201 aactttttcg acaggcacct caagggcgcg gatgtcgaga tcaagctggt gccggaggac
     4261 gagctggctg tcgagccgcc aaaaccagcg gtgaagtagg aaagttcgcc gaactctggc
     4321 atcgaatcgc ctcatcaagc ccgcggcgcg ttttcaaacg ccgagcatgc cggtcacgga
     4381 ctcgcgcttg aggcgcagct cgcgccattc gtggtcgaag gcgctgccgc tgacgatgcc
     4441 gcagcccgat gggagctggg cctcgctgcc ctgccagccg atgccgcgta tggcgacgat
     4501 gacatggcac ggcgagtccg cccccggttc gatgaatccg aacggcgctc cgaagaagcc
     4561 cggcacctgg agctgctggc ggtaatcgcg caggcgcgcc agccagtgct cgtcgcgcgg
     4621 caggcagccg accgcgggag tgggatgcag gtggcggatg agggcgtcgg cggaggacgg
     4681 tgacttgagg gcgagggtca gccgggactg gaagtgcagc agccccgaag cctcgcacag
     4741 gccgcgcggc tcgcgcacga catctcccag catgccgagg cgctccagca ggtagcgcac
     4801 gacgatctcg tgctcctcga tctccttcac atcgctgagg aaatcggccc ggtctccggg
     4861 cttggcggtg ccggccaggg ccatggtctc gatctgcgcg ccccgcatga ggaagagcag
     4921 ctcggggctg gcgcccagaa agccgctctc ctgcccgatg aaggcgtaac cccaggtgtt
     4981 cgagggggcc tccatgacgc gctccagcag gcgcaccggg tgcccggctg tgatcgtgcc
     5041 gcgctcggtg agcacgggga ccattttttc caggcggcgt ccgagaacct cccgccggat
     5101 gcgcctgaag gccatcttga accattcggt cacgggtttg gcccagatga tgcgcggcgg
     5161 ctccgcgccg ttccaaccgg cctgggagcg cagggtggcg ggtgtgatct cgacgagccg
     5221 cgcaggccgt ttccagggca gcgggtcgcg caggtcgaag tcattgacat aaaagcagcc
     5281 gccggcggac gggggttcag cgagggcctc gaaagggccc gcgcccagcc aggcccttcc
     5341 gccgggcagt ctgaaaagtg cgagttcggt cattgtcttc cctgatagtg aaacggatgt
     5401 cccccggcgc aagaggcgag gatttgcatt tccgatttgt cttcatgcat tgagatggca
     5461 caaggggctg cgtttgccgg cctcgcgttc ttttttctgt ccaaaacgac gcgcgcgccg
     5521 actctaaaat gcccagccat gcgcaaccgc cgtcaattca tctccgccgt caccgccgcc
     5581 ctgggcggtt ctgtttttgc cgccgatggc agaccgaaga ctctcgtcct gcaatccgcc
     5641 tgggacacgg tcaacatcgg cgacatcggc cacacgccgg gcacgctgcg gatcatcggg
     5701 gagcatctgc cggggctgaa ggtcgtgctt tgggcgatga agctcgacga gcgcgtcacc
     5761 gccatgctga aggcgcgctt cccggacgtg gagatcgtcc agggcagcct ggtcaaggag
     5821 acgccggaga gcgcccgtct tcacgaggcg gtgaggacat gcgatctgtt catccgcaat
     5881 tcgggcatgg gccaggacat cagtttcatg cagttctgcc ggaagcacgg caagccctac
     5941 ggccttttcg gccagtcgta cttcccgaca atggtcagcg gcgagggggc ggaggagcgc
     6001 ctggacctgc tcaacagcgc cgccttcatt tacacgcgcg agaccaggac actcggcatc
     6061 ctgaaaaagg ggggcgtgaa gtgtccggtg atggagttcg gtccggacgg ctgctttggc
     6121 attgatgtgc gggatgacac gcgcggtctg gagacgatgc ggaagctggg gctggaggag
     6181 cgcaagttca tcaccgtgca aatccgcacc aacacgccca agtatccggg cgtggatgat
     6241 ccgcgcccgg agaagctcaa tcccctgcat cccacgccgc ggcagaccgc cgatgacgag
     6301 cgccgcgcgg cgaagtaccg cgagctggtc acccgctggg tcagggagac cgggcacaag
     6361 gtgctcatcg ccccggagac gatcaaggag atgggccaca acaagcgtct catccacgac
     6421 ccgctgcctc cggagatcca aaagcatgtg gtgaacctcg agtacttctg gaacgccgac
     6481 gaggccgcgg ccatctttgc ccgcgcctgc gtcatcatct gccacgagcc gcactcgccc
     6541 atcatcggcc tggcgaacgg cacgcccatc atccacacct actccgagtt ccacagcccg
     6601 aagtgctgga tgtttcagga catcggcctt cctgagtggc tgctggagat ggatgaaact
     6661 cccgtcgaaa aaatggcgga gacccttttt gccattcatg ccgatcccgg agccgcccgc
     6721 gcgaaggtgg aaagggccat gtcattcgtg catgagtgct tcggcaaatc catgcggcat
     6781 gtccgtggcg tgctggcagc gtgaggctgg ggcggaagga ctccggcaaa ccgcgccttt
     6841 ggatggctgg aggagaggct tcccgccagg cgtggttgaa ctttttttga gaaagagtgt
     6901 aacggtggag gagaaaaacg gtattcccaa gtgtgggggc attgtttcct gccgctgaat
     6961 ggatgagcgc gttgagcacc gggccatctt tgtcacggag ggacagggct gtcacaactg
     7021 ggttttgcat ggtttgctcc cggtcataca tggaggcggc ggcgcctggg ttgggcgccg
     7081 ccgcttctct tgtccgcggg tgtgtgagat ccgacgccgg ggcgtctcaa tgcttgagca
     7141 gccagtcggt ggtgcggggg ttgaagtcgc tcaggcattc ccagtggaag gcgtgcccgg
     7201 cattgtcgta aaggtggagg tcggctccgg ggatggcggc ggcgacttcc tcgctcatcc
     7261 atttgggagt gaaggcatcg tccttgccgc cgatgacgag cgcggggcat ttgacgtttt
     7321 tcagctcgcc gagggtgttg tgcgtgatgg cggcggcgct ctgcgcctcc atggcgtgga
     7381 cgggctgggg ggcggggttg gtggcggcgt ctttgaggcc ctgctgcatg ttctcccagc
     7441 agtcgtcgtt gtcgaaccag ggcttggtga agatgagcat ctgcacatag tgcatgaagt
     7501 cctcggggcg catggaggct ttgcatttca tgaggtgctg ccaggtgtaa aggccgaagc
     7561 ggtcctgacg cgcccaggta cacatgagga tcatgctttt caccttctgc ggatggcgca
     7621 gggcgagctg ctgtgcgatc acggagccga gcgagcagcc gacgacgcgg gcctggctga
     7681 tgccgagcgc gtccatgagt ccggcgtagt cgtcggccat catggcggtg gtgtaggggc
     7741 cttcaggctt gtcggtctgg ccgacgcctc ggttgtcgcc gaggatgcag cggaagtgct
     7801 tggaccattc ggcggcgtgc gcctcccaga cggcgccggg cgcggtgacg cccatgatgc
     7861 agaccagcgg ctcgccgctg ccgcgctctt cataatacat ctggattccg ttggtggtga
     7921 cgtggggcat ggtggtgggt gggggagatg tttgaaaaat gaaattcgaa aaacaaaact
     7981 cgaatatcga acttcggatt tagcggctgg ggagcctgcg gccaaggaaa aattactgtt
     8041 cccgcgcgga ccggcgcggc cttacttgag gaaacctttg agcaggtctc cgaggccctg
     8101 gccgctcatg aggttcttca gcgcgcggtc ggtgtcggga gtgaccttgg gatcggagag
     8161 ggagccgcgg gatttgatgt cgaaggtgag gcgtccgcgc ttgtcggaaa gggcggcgat
     8221 gaggccgcgc gcgaagtccc tgccaagacg ggcgccggcg atgccggtgt ggaggcgctc
     8281 ctggagttcg gggccgcagg agaggcggat gtcgagttcg tgggtgtccc tgccggtgtc
     8341 cagccaggac ttgggcgcga gcgccacttc gtagtcgggg aagacgaaaa ggcagccctg
     8401 cggcaggatg agggcgccat tgacccagcg cgcctggatg ggcgcgtcct gctggagcgg
     8461 gccgccaatg ggcacggggg ccaggtcaat gccgaactcc tcaagcttgc gcagatcctt
     8521 gcccgccgcg tcgccgatgg tctgctgccc gccgaggatg gagcctttgg cgagcgtgag
     8581 ggtgagcagc gcggagggct tccactcgcg agtctgcggg tgaagagggg tgatcctacc
     8641 ctcgccggtg agatcgagca gggcgatctc agccttgcgc ttgacgccct ggatgcgcgt
     8701 catgccggtg acggcgacat gcgccttgat ggaggtgtcg agttcgtcgc ctggcttgag
     8761 agtgtccatg tccaggccgc tgatgctgta gtcgagaccg gtgatgtgga cagtggtgtc
     8821 cctggcgttc atgaaaaacg agccgcgctc gatgcgggcg gcggccaggg cgaagctgaa
     8881 cgcggcgggg gcttcgtcca gcggcaggg
//
LOCUS       DAOMYJ010000210         8884 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Prosthecobacter sp. isolate AMR_MDS_5629
            NODE_2614_length_8884_cov_19.666780, whole genome shotgun sequence.
ACCESSION   DAOMYJ010000210 DAOMYJ010000000
VERSION     DAOMYJ010000210.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867850
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prosthecobacter sp. (wastewater metagenome)
  ORGANISM  Prosthecobacter sp.
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Verrucomicrobiaceae; Prosthecobacter.
REFERENCE   1  (bases 1 to 8884)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8884)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.30
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:34:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,742
            CDSs (total)                      :: 4,684
            Genes (coding)                    :: 4,676
            CDSs (with protein)               :: 4,676
            Genes (RNA)                       :: 58
            tRNAs                             :: 55
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8884
                     /organism="Prosthecobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2614_length_8884_cov_19.666780"
                     /isolate="AMR_MDS_5629"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1965333"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..433
                     /locus_tag="PLP58_17620"
     CDS             <1..433
                     /locus_tag="PLP58_17620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ10403.1"
                     /translation="LGFGPVLAGTSAEWAFDEARVEELRRVSQASGGRELLEMSDAWR
                     SPAIPRFTDLTLPLLVLTLVLLLFEALVTRTGWRLPELVVKPARPTASKAAEKEARQH
                     RQRLDSGAAPQPAPSASPAPAETPPSTPDERQSRYQRAKRR"
     gene            479..1036
                     /locus_tag="PLP58_17625"
     CDS             479..1036
                     /locus_tag="PLP58_17625"
                     /inference="COORDINATES: protein motif:HMM:NF017496.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Uma2 family endonuclease"
                     /protein_id="HRJ10404.1"
                     /translation="MPATLTHRFTVDDYYRMAETGILKPGARVELIEGEIVDMMPIGP
                     FHSGSVYRLTRIFSRLSKSRWLVASQGPLRLDKHNEPEPDLMLLRPAKDDYTSAHPGP
                     DDVFLVIEVADSSLAYDQTTKLPLYARFGIEEVWILNVPQKQLEIYRAPNFLGYESKI
                     ILHEGKAAPARFPDAEVDVTELLST"
     gene            complement(1070..4408)
                     /locus_tag="PLP58_17630"
     CDS             complement(1070..4408)
                     /locus_tag="PLP58_17630"
                     /inference="COORDINATES: protein motif:HMM:NF019223.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PSD1 and planctomycete cytochrome C
                     domain-containing protein"
                     /protein_id="HRJ10405.1"
                     /translation="MIPRSHILQIVLGGAFAVGPGLAAESFTAEQIEFFEKSVRPVLA
                     ENCHDCHGGHKHENGLRVDLRSAILRGSDYGKVVEPGNPAASKLIKAIQHAPGVEAMP
                     KKGGKLQPQEIAALEKWVQMGLPWPEEKEVAHGPAKDPMQHWAFQPVKKPALPEIRNS
                     KFETRNPIDAFIAAKLEAAGLDFAPPADAAALCRRLHVTLTGLQPSFEEVKAFEMAAA
                     RDLQAAVRELSNRLLNSPAYGERWARHWLDVVRYADTDGYQVAGKNNRYPYAYTYRDW
                     VVDSLNRDMPYDEFLTHQLAADKLHPEDPAHPSLAALAFLNVGDRFINNRDLQTDDRI
                     DVVTRGMLGLTVGCARCHDHKYDPIPSKDYYALYSVFNSSIEPANEALPVIGKAADEG
                     EAKDYQDKLDEIARKEMDFKRKVYDEIRAPERLVEYLAFIREAQDIKDGTARKGRAGQ
                     LKLRDKVADKWEDFLKRHALKDKPHPVMLAWKEFIGLPEKEFAAKAPAIVEKLVKPEA
                     GLGGVPRNELAKRPAPKSFQDIAQMYADIFNTCLAGTEPENDDWRQVREILQAEPSPM
                     AFPVERMNEFFTRKDLDSVVKIANERVKLDVEHTGAPPRAMVMLDRDKPADVRIFIRG
                     NPSRQGEVSPRGWLTMFGGEKFTDGSGRLELARKIASKDNPLTARVIVNRVWAQHFGK
                     PLVGQPSDFGVQTDAPAQQEVLDYLAAALMEEGWSLKKLHQRIVTSRAWQQASRVTAE
                     KALKDADNELISRQNRQRLDYEAMRDAMLQVTGELNAAQVGGRALALNDKAADTRRTL
                     YLLVDRYEQATVPAMFDFANPDSHSPMRYNTTVPQQALFLMNSPFLRQRSGTLAEKTP
                     VQGSAIDSQSIEALYQRVLRRSPSPSEVELAQRFCNDADTLGKRAFAFVWSYGWGAVE
                     KDPATGKASVTGFTALPHFDASSKRWTPGKTFPDKEHGHLYLAPGNGHPGGTGKSAIM
                     QWTSPLAGEKIRVTGAAKRSSDKGNGVRAWIISNHAGALKEVFIKPASSSEIAVETTV
                     QEGEVLSFVVEAENGDTNSDSYSWAPRIERLDPVTGQAVLLTKSDTDFCGPAGWPLNR
                     EKPQSALAQLAQVLLMSNEFQFVD"
     gene            complement(4550..5752)
                     /locus_tag="PLP58_17635"
     CDS             complement(4550..5752)
                     /locus_tag="PLP58_17635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity protein TolA"
                     /protein_id="HRJ10406.1"
                     /translation="MKPDYQVRLLHPKRIQDSDRGKLRETATLDGIIRVGLYGRLAEQ
                     FTAAMTEDKPAAVSGMRLYLDGVAMEGLQPAIFQPEAANDVLPKPAWVLEFQLTRTAQ
                     DEANRKAWDALLPRLSFGGNAVQIGVGFNGQPAHPGDGRELSFQAVSKGKAWAVILCG
                     LSLLVFVLVWAVRSGMLREAGPGTAYSLGKTQMAFWGLLVVLSFFGVWIVSGKMERIP
                     DQVLVLVGISGTTGLAAMLIGDGKKTAAAQKAEELRGKIAEDAAKLAEIEARKTAMEN
                     ERKAARETWPVEKEAILASLSMEAGSQAQALKKSREELAAREKESRAAPADKQWSAWF
                     TDIISDASGPSFPRVQMMLWTLVLGAVFVKSVGTAFTMPEFDNTLLLLMGISNGTYLG
                     FKMPEKNG"
     gene            6053..6718
                     /locus_tag="PLP58_17640"
     CDS             6053..6718
                     /locus_tag="PLP58_17640"
                     /inference="COORDINATES: protein motif:HMM:NF019349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HRJ10407.1"
                     /translation="MPASSSPSPVRLQSRAYAELRRLIASGEFPPGTFLSERQLAAQL
                     GMSKTPVHVALERLESEGFVTISAQQGVVVRGLSVEDIVDHYELREAVESWVVRRLAG
                     RLKPAQVEELNVNLASQRQALAENDLRELMRLDEQMHYLLASYLGNREILSTMERLRD
                     KIHQVILRVTDTDRARPAESTAEHETIIAAIIAGQPDEAARLMIAHLEAGKRRILNPE
                     RFA"
     gene            6720..>8884
                     /locus_tag="PLP58_17645"
     CDS             6720..>8884
                     /locus_tag="PLP58_17645"
                     /inference="COORDINATES: protein motif:HMM:NF019223.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PSD1 and planctomycete cytochrome C
                     domain-containing protein"
                     /protein_id="HRJ10408.1"
                     /translation="MNARLVGVQALAVLLAGFQALAAPDFTRDIQPIFEARCVQCHGP
                     EEQNGGLRYDQKARAFVQADSGHHAIVPGKPDQSELLKRVTSTHKNDQMPPKGARLSP
                     RQIETLTAWIAAGAEWPETAQPVAENKPASTHWAFQPLTDPPLPEIKDKTWPLNPIDH
                     FIQAKREAAGLTPAPDAEPHVLARRLSYALTGLPPDKFEFRISNFELSAALESLLSSP
                     HYGERWARHWLDWVRYADTAGDNSDYPIPQARLYRDYVIQSLNDDLPYDRFLTEQLAG
                     DLLPAASQEEKNRQTIATGYIALSRRFGSLIERYPWHVTIEDTLDNLGRTVMGLTLSC
                     ARCHDHKFDPVTMRDYYGLYGIFASTRYPQPGLELFKAQHHFPPLIPEKEAAAKLAPF
                     EKETRRLEADLTQRLAADEAKAIEFARLEKTASLDEQRRLKGELDRQLRRTRDAGEDL
                     AKHLKKLPLIPAAYAVSEAAPSNARLQMRGEPERPGAEVPRKFPDILGGHALPADIAA
                     KTSGRLQLAQWITDPKNPLTARVIVNRVWQRHFGKGLVPTTSDFGLRGEPPTHPDLLD
                     WLATDFIRHGWSLKHLHRRILTSRTWRMASTDSADNLASDPGNTLYWKFNRQRLDAES
                     LRDTLLLLSGQLDLTPQDKPFPFPAMKDWGYTQHHPFKDDYPSNKRSIYQMTKRLTAA
                     PYMQTFDGPDPNACTATRDSSVTALQALHFLNDETVHTTA"
BASE COUNT         1584 a         3070 c         2583 g         1647 t
ORIGIN      
        1 gctgggcttc ggccccgtgc tggccggcac gagcgccgag tgggcctttg acgaggcgcg
       61 cgtggaggag ctgcgccgcg tctcccaggc cagcggcggg cgcgagcttc tcgaaatgag
      121 cgacgcctgg cgcagcccgg ccatcccgcg tttcacggac cttacactgc ccttgctcgt
      181 gctgacgctc gtgcttttgc tgttcgaggc gctcgtcacc cgcaccggct ggcgcctgcc
      241 cgagctggtg gtcaaacccg cgcgtccaac cgcatcgaaa gccgccgaaa aagaggcgcg
      301 ccagcaccgc cagcggctcg actccggtgc cgcgccccaa cccgcgccat ccgcctcccc
      361 cgcaccggcg gaaaccccgc cctccacacc cgacgaacgc caaagccgct accagcgcgc
      421 gaagcggcgt tagttgcccg cccttgcatt cgaatccgca gcgcgcatca ttcccgccat
      481 gcccgccaca ttgacgcatc gtttcacggt ggatgattac taccgcatgg ccgagaccgg
      541 gattttgaag cccggcgcgc gggtcgaact cattgaaggg gagattgttg acatgatgcc
      601 cattggtccg tttcacagcg gctcggttta tcgcctgacc cgcatcttca gccgcctctc
      661 aaaaagccgc tggctcgttg ccagtcaggg ccccttgcgg ctggacaagc acaatgagcc
      721 ggagccagat ctcatgctgc tgcgccccgc caaggatgac tacacctcgg cgcatcccgg
      781 ccccgacgat gtcttcctcg tcatcgaagt ggcggactcc agcctggcgt acgaccagac
      841 gaccaagcta ccgctctacg cccgctttgg cattgaggag gtgtggattc tgaacgtgcc
      901 gcaaaagcag ttggagattt accgcgcgcc gaattttctc ggttacgaat ccaaaatcat
      961 ccttcacgag ggcaaggccg cccccgcgcg cttcccggac gccgaagtgg acgtgacgga
     1021 actgctctcg acttgaaagc ggttaaaacc ggcgccacgc gaccagtgct cagtcaacaa
     1081 actgaaactc gttggacatg agcagcacct gggcaagttg cgccagcgca gactgcggct
     1141 tctcacgatt caatggccaa ccggcagggc cgcagaaatc ggtgtccgat ttggtgagca
     1201 gcacagcctg accggtgacg ggatcgagtc gctcgatgcg cggcgcccag gagtagctgt
     1261 cgctattcgt gtcgccattc tcggcttcaa ccacgaagct gagcacctcc ccttcctgaa
     1321 cggtggtttc gacggcgatt tcgctgctgg aggcgggctt gatgaagact tccttcaacg
     1381 cgccagcatg gttggagatg atccaggcgc gcacgccatt gcccttgtcg ctggagcgct
     1441 tcgcggcgcc ggtgacgcgg attttttcgc cggccaaagg cgaggtccac tgcatgatcg
     1501 ccgatttgcc cgtgcccccg ggatggccgt tgccgggtgc caggtaaaga tggccgtgct
     1561 ctttgtcagg gaaggttttt cctggtgtcc agcgtttgct ggaggcgtcg aaatgcggca
     1621 gtgcggtgaa gcctgtcacg ctggctttgc cagtggcggg gtccttttcc acagcgcccc
     1681 agccataact ccaaacgaag gcgaaggcgc gcttgccgag ggtgtcggca tcattgcaga
     1741 agcgctgggc cagctccact tcggaagggc tggggctgcg gcgcagaacg cgctgataga
     1801 gtgcttcgat ggactgggag tcaatcgcgc tgccctgcac gggcgtcttt tccgccaggg
     1861 tgccggaacg ctggcgcagg aaggggctgt tcatcaggaa gagcgcctgc tgcggcacgg
     1921 tggtgttgta gcgcatcggg ctgtggctgt ccggattggc aaagtcgaac atggcgggca
     1981 cggtggcctg ctcgtaacgg tccacgagca ggtagagcgt gcgccgggta tcggcggctt
     2041 tgtcattgag cgcgagcgcg cgcccgccga cctgggcggc gttcagctcg ccggtgactt
     2101 gcagcatggc gtcgcgcatg gcctcgtaat cgagacgctg gcggttctgg cggctgatga
     2161 gctcgttgtc ggcgtctttg agggctttct ccgcagtgac gcggctggcc tgctgccagg
     2221 cgcggctggt gacgatgcgc tggtgcagtt tcttcaggct ccagccttcc tccatgagtg
     2281 cggcggccag ataatcgagc acctcctgct gcgcgggcgc gtcagtctgc acaccgaaat
     2341 cgctcggctg gccgaccagc ggcttgccga aatgctgcgc ccatacgcgg ttgacgatga
     2401 cccgcgcggt aagcgggttg tccttgctgg cgatcttccg cgccagctcc agccggccgc
     2461 tgccatcggt gaacttctcc ccgccaaaca tcgtcagcca tccgcgtggc gacacctcgc
     2521 cctggcggct gggattgccg cggatgaaaa tgcgcacgtc ggcgggcttg tcgcggtcca
     2581 gcatgaccat ggcgcgcggc ggcgcaccag tgtgctcgac gtccagcttc acgcgctcgt
     2641 tggcgatctt gaccacgctg tccaagtcct tgcgggtgaa aaactcgttc atccgctcga
     2701 cggggaaggc catcgggctc ggctcggcct gcaaaatctc gcgcacctgc ctccagtcgt
     2761 cattctcggg ctcggtgcca gccagacatg tgttgaaaat gtcggcatac atttgggcga
     2821 tgtcctggaa gctcttcggc gcggggcgtt tggccagttc gttgcgcggc acgccgccga
     2881 ggccggcctc gggtttgacg agtttctcga cgatggccgg cgctttggcg gcgaactcct
     2941 tctcgggcag tccgatgaat tccttccatg ccagcatgac ggggtggggc ttgtccttga
     3001 gggcgtggcg tttgaggaag tcctcccatt tgtcggccac cttgtcacgc agcttgagct
     3061 ggccggcgcg tcctttgcgg gcggtgccgt ctttgatgtc ctgcgcctcg cggatgaagg
     3121 caagatattc caccagccgc tccggcgcgc ggatttcatc atagaccttc cttttgaagt
     3181 ccatctcctt ccgggcgatc tcgtcgagct tgtcctgata gtccttcgcc tcaccttcat
     3241 cggcggcctt gccgatcacc ggcagcgcct cattggcggg ttcgatgctg ctgttgaaga
     3301 cggagtagag cgcgtagtag tccttgctcg gaatgggatc gtatttgtgg tcgtggcagc
     3361 gcgcgcagcc gacggtgagg ccgagcatgc cgcgcgtgac cacgtcaatg cggtcgtcag
     3421 tttgcaggtc cctgttgttg atgaagcggt cacccacgtt gagaaatgcc agcgctgcca
     3481 gcgaaggatg cgcggggtct tcagggtgga gtttgtcggc ggcgagttgg tgggtgagga
     3541 actcgtcata cggcatgtcc cggttcagcg agtccacgac ccagtcgcgg tatgtgtagg
     3601 cgtaggggta gcggttgttt tttcccgcca cctggtagcc gtcggtgtcg gcgtaacgca
     3661 ccacatccag ccaatgccgc gcccagcgct cgccgtaagc gggggagttc agcaggcggt
     3721 tcgagagttc gcgcacggcg gcttgcagat cccgcgcggc ggccatttcg aatgccttca
     3781 cttcttcaaa cgaaggctgc aggccggtga gcgtcacatg caggcggcgg caaagcgcgg
     3841 cggcatccgc aggcggggcg aaatcaagac cggcggcttc cagcttggcg gcgatgaagg
     3901 cgtctatggg atttcgagtt tcgaatttcg aatttcgaat ttcgggaagg gcgggctttt
     3961 tcaccggctg aaaggcccag tgctgcatgg ggtccttggc aggaccgtgc gccacctcct
     4021 tttcctcagg ccagggcagg cccatttgca cccatttttc cagcgcggcg atctcctgcg
     4081 gctggagttt gccgcccttt ttcggcatgg cttccacgcc gggcgcgtgc tggatggctt
     4141 tgatgagctt ggacgcggcg gggttgccag gctcgacgac cttgccgtaa tcactgccgc
     4201 gcaggatggc ggagcgcagg tcaacacgca ggccgttctc gtgcttgtgc ccgccatgac
     4261 agtcgtggca gttctcggcc agcacggggc gcacgctttt ttcaaaaaac tcgatctgct
     4321 ccgccgtgaa gctctcggcg gcaagacccg ggccgacagc aaaggcgccg cccaggacga
     4381 tctggaggat gtgcgaacgt ggaatcattc agaggggatt tgcgcgcgaa ccatgccgcc
     4441 gcatggacag ctacgcgcag gaaggtggcc atgttgcacc tggcatgttt gcccgcaatg
     4501 aggcatttgc ctcaaggttc gcgggctgcg tcgctcggac agacgggtct cagccgttct
     4561 tctccggcat cttgaatccg aggtaggtgc cgttgctgat gcccatgagc aggagcagtg
     4621 tgttgtcaaa ctcgggcatc gtgaacgccg tgccgacgct cttcacaaag accgcgccaa
     4681 gcacgagcgt ccacaacatc atctgaaccc tcggaaaact ggggccgctg gcatcggaaa
     4741 tgatgtccgt gaaccaggcg gaccactgtt tgtcagccgg agccgcccgg gattcctttt
     4801 cacgcgcggc cagttcctcc ctgctctttt tcaatgcctg ggcctgtgat ccggcttcca
     4861 tgctcaagct cgcaagaatc gcctcttttt cgacgggcca tgtctctctg gcggccttcc
     4921 tctcattttc catggcggtc tttcgcgcct cgatttccgc cagtttggcg gcatcttcgg
     4981 cgatctttcc gcgaagctcc tccgccttct gcgccgccgc ggtctttttg ccgtcgccga
     5041 tgagcatcgc cgccaggccg gtggtgccgc tgatgccgac gagcacgagc acttggtcgg
     5101 gtatgcgctc catttttcca ctcacgatcc agactccgaa aaacgacaac accacgagca
     5161 gtccccagaa ggccatttgc gtctttccaa gactgtaagc cgtgccggga cccgcctcgc
     5221 gcaacattcc tgaccgcacc gcccaaacca ggacgaagac cagcaaactg aggccgcaca
     5281 ggatcactgc ccatgctttt cccttcgaca cggcctggaa agacagctcc ctgccatccc
     5341 ccggatgggc cggctggccg ttgaaaccca caccgatctg aaccgcgttc ccgccgaaac
     5401 tcagcctggg aagcagggcg tcccaggctt tccggtttgc ctcatcctgc gctgtgcggg
     5461 tcaattggaa ttccagcacc caggctggct tcggcaggac atcattcgcc gcctcaggct
     5521 gaaaaatcgc gggctgcaat ccctccatcg ccaccccgtc gagataaagc cgcatgccac
     5581 tgaccgcggc gggcttgtct tccgtcatgg ctgcggtgaa ctgctccgcc agcctgccat
     5641 aaagcccaac ccggatgatg ccgtcgaggg tggcagtttc gcgcagcttg cctctgtcgg
     5701 aatcctggat gcgcttcgga tgcagcaacc gcacctggta atccggcttc aaagattcag
     5761 acggcggtgc gtcaccttga gcatggagtg ctgtatgcag aaatagcagc gaaaagcacg
     5821 ggatgacgga ggcgggagca aatctgggaa tcatcgtgtg atggaatggc ggattcatca
     5881 ggcagctcct ctgtgagcaa ccatcgagcc tcattactcc ggacgagact aaaaatgtcg
     5941 aggcaattct cgacacggcg acgtgattta gccccggcaa agacggcgcg attcattccg
     6001 cgtgaaagat tttcatctcc cccgttcgta cctggcatgc cagtcagatt ccatgcccgc
     6061 ctcatcatcg ccttcccctg tccgtctgca atcccgcgcc tacgccgagc tgcggcggct
     6121 gatcgcttcc ggcgagtttc cgccgggcac gtttctctcc gagcgccagc ttgccgccca
     6181 gcttggcatg tcgaagacgc ccgtgcatgt ggcgctcgaa cggttggaat cggagggctt
     6241 tgtcaccatc tcagctcagc agggcgtcgt cgtgcgcggg ctgagcgtgg aggacatcgt
     6301 ggaccactac gagctgcgcg aagccgtcga atcctgggtg gtgcgcaggc tggcaggccg
     6361 gctcaaaccg gctcaggtgg aggaattgaa tgtcaacctc gccagccagc gccaggcact
     6421 cgcggaaaac gatctgcgcg agttgatgcg cctcgacgag cagatgcact acctgctggc
     6481 ctcctacctc ggcaatcggg agatcctctc caccatggag cgcctgcgcg acaagattca
     6541 ccaggtcatc ctgcgcgtca ccgacaccga ccgcgcccgc cccgccgagt ccaccgccga
     6601 gcacgagacc atcatcgccg ccatcatcgc cggacagccc gacgaagccg cgcgcctcat
     6661 gatcgcccac ctcgaggccg gcaagcgccg cattttgaac cccgaacgct tcgcctgaaa
     6721 tgaacgcgcg tcttgttggt gtccaggctt tggccgtttt gcttgcgggt tttcaagcgc
     6781 ttgccgcccc cgacttcacc cgcgacatcc agcccatctt tgaagcccgc tgcgtgcaat
     6841 gtcacggccc cgaggagcaa aacggcggtt tgcgctacga tcaaaaagcg cgcgcctttg
     6901 tccaggccga ctccggccat cacgccatcg tccccggcaa accggatcaa agcgagctgc
     6961 tcaaacgcgt cacctccacg cacaaaaacg accagatgcc gcccaaaggc gcgcgccttt
     7021 ccccgcggca aatcgaaacc ctcaccgcct ggatcgccgc cggagccgag tggccggaga
     7081 ccgcgcagcc cgtcgccgaa aacaagcccg cgtccaccca ctgggccttc cagcccctca
     7141 ccgatccgcc cctccccgag atcaaagaca aaacctggcc gctcaacccg attgaccact
     7201 tcatccaggc caaacgcgaa gccgccggcc tcacccccgc gcccgatgcc gagccgcatg
     7261 tcctggcccg ccgcctctcg tatgcgctga ccgggctgcc gccggacaaa ttcgaatttc
     7321 gaatttcgaa tttcgaactt tccgccgccc tcgaatccct cctgtcctcc ccccattacg
     7381 gcgagcgctg ggcgcgccac tggctcgact gggtccgcta cgccgacacc gccggagaca
     7441 actccgacta tcccatcccc caggcccggc tttaccgcga ctacgtcatc cagtccctca
     7501 acgatgacct gccctacgac cgcttcctca ccgagcagct cgcaggcgac ctcctgcccg
     7561 ccgcgagcca ggaggaaaaa aaccgccaga ccatcgccac cggttacatc gccctctcgc
     7621 gccgttttgg ctccctcatc gagcgctatc cctggcatgt caccattgag gacacactcg
     7681 acaacctcgg ccgcaccgtc atgggcctca ccttgagttg cgcccgctgt cacgaccaca
     7741 agttcgaccc cgtcaccatg cgcgactact acgggctcta cggcatcttc gccagcacgc
     7801 gctatccgca gcccggcctc gagcttttca aagcccagca ccatttcccg ccgctcatcc
     7861 cggaaaaaga agccgccgcc aagctcgccc ccttcgaaaa agagacccgc cgcctggagg
     7921 ccgacctcac ccagcggctc gccgccgatg aagccaaggc catcgaattc gcccgcctcg
     7981 aaaaaaccgc cagcctcgac gagcagcgcc gcctcaaagg cgaactcgac cgccaactcc
     8041 gccgcacccg cgacgccggc gaagaccttg ccaagcacct caaaaaactc cccctcatcc
     8101 ccgccgccta cgccgtcagc gaagcagcac cctccaacgc ccgcctgcaa atgcgcggcg
     8161 agcccgagcg ccccggcgcc gaagtcccgc gcaaattccc cgacatcctc ggcggccacg
     8221 ccttgcctgc tgacatcgcc gccaaaacca gcggccgact ccaactcgcg cagtggatca
     8281 ccgatccgaa aaacccgctc accgcccgcg tcatcgtcaa ccgcgtctgg cagcgccact
     8341 tcggcaaagg cctcgtcccc accaccagcg acttcggcct gcgcggcgag ccgcccacgc
     8401 atcccgactt gctcgactgg ctcgccaccg acttcatccg ccacggctgg tccctcaagc
     8461 acctgcaccg ccgcatcctc acctcccgca cctggcgcat ggcctctacg gacagcgcgg
     8521 acaacctcgc ctccgatccc ggcaacaccc tctactggaa attcaaccgc cagcgcctcg
     8581 acgccgaatc cctgcgcgac accctcctcc tgctcagcgg ccagctcgat ctcacaccgc
     8641 aggacaagcc cttccccttt cccgccatga aggactgggg ttacacccag caccacccct
     8701 tcaaggacga ctaccccagc aacaagcgca gcatctacca gatgaccaag cgcctcaccg
     8761 ccgcccccta catgcagacc ttcgacggcc ccgaccccaa cgcctgcacc gccacccgcg
     8821 acagctccgt caccgccctg caagccctcc atttcctcaa cgacgaaacc gtccacacca
     8881 ccgc
//
LOCUS       DAOMYJ010000211         8774 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Prosthecobacter sp. isolate AMR_MDS_5629
            NODE_2655_length_8774_cov_22.319073, whole genome shotgun sequence.
ACCESSION   DAOMYJ010000211 DAOMYJ010000000
VERSION     DAOMYJ010000211.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867850
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prosthecobacter sp. (wastewater metagenome)
  ORGANISM  Prosthecobacter sp.
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Verrucomicrobiaceae; Prosthecobacter.
REFERENCE   1  (bases 1 to 8774)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8774)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.30
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:34:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,742
            CDSs (total)                      :: 4,684
            Genes (coding)                    :: 4,676
            CDSs (with protein)               :: 4,676
            Genes (RNA)                       :: 58
            tRNAs                             :: 55
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8774
                     /organism="Prosthecobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2655_length_8774_cov_22.319073"
                     /isolate="AMR_MDS_5629"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1965333"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(103..1344)
                     /locus_tag="PLP58_17650"
     CDS             complement(103..1344)
                     /locus_tag="PLP58_17650"
                     /inference="COORDINATES: protein motif:HMM:NF012694.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HRJ10409.1"
                     /translation="MPSFTYTALNPSGQTITGSLAVGSRAEAFRKLEAQALTPVKVME
                     EAKAAKAGAASSASQAAPNEPVKLKRAQIILFTEELADLLDGGLQIDQALRVMNERQE
                     APALRQVSAILREHIREGATVAKALKKASPSFDDLYCNLVAAGEVSGTLAPILRRLAQ
                     NLAVIAELQAKVTQAMIYPAFLIGACAVLMIVFMTVMVPQITDLLAKSGQKLPFATQM
                     LISFNNFLASWWWVMLSVVVILALSFKAYIATPKGLMWWHQTKLHTPLFGPVIATRFY
                     AQFAHSMGSLVGNGVPLLNSIRLVSKISANVFIQSLLARVNNLVSEGSSLSNSLKKVG
                     HFPMMLVDMIAIGEQTGEIGKSLEKAATRYDKELDKRIKRMTALISPVIILFMAVIVG
                     VVAYSIISAVFQSVNGIKSRV"
     gene            complement(1431..3125)
                     /locus_tag="PLP58_17655"
     CDS             complement(1431..3125)
                     /locus_tag="PLP58_17655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009962525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase, T2SS/T4P/T4SS family"
                     /protein_id="HRJ10410.1"
                     /translation="MVTTTLPDILIQAAADAGCRDTSRLRHTLEDAADKRQPLIEALL
                     DANLVDEEAFFETLAGTLQMPFARENGEEQEGLHARFPAKLALRHRICPAHVSSVEAT
                     LLTYNPFDLGARQAVGQELGKRVHWQIASRHRILESLHQGYGVGAENFEELLEGREGI
                     EDDDDLKQETTVLDEADEEATVMNFVNQIFREGLKERATDIHVEPLERDLRIRYRIDG
                     KLIEVPVPPNMRLLQNSLVSRLKIMAHLDIAERRLPQDGRINLELDGDPIDVRVATIP
                     SVNGESVALRLLTRKKFDMAQLGLDAQTESIFRRLLAQPNGIILVTGPTGSGKSTTLY
                     TLLKELNTKDRRIVTIEDPVENKLDGVVQIAVKPEIDLTFAAGLRSILRGDPNVIMVG
                     EMRDQETVEIAIRGALTGHLVFSTLHTNDAVGGISRLLDMGIEPFMISSSVRAFQAQR
                     LVRTLCPYCKKPAHYEDGHLKSMGFPMEWKGQLWQAGGCRSCRNTGFSGRLAIMEICQ
                     MTGALQEKINARATTAELKAQALQDGMIPMRHYGFRKAAEGITTLEEVMTVTAAGE"
     gene            complement(3153..3749)
                     /locus_tag="PLP58_17660"
     CDS             complement(3153..3749)
                     /locus_tag="PLP58_17660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018968884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HRJ10411.1"
                     /translation="MAEVPSTRILPLGSPAPEIELPDAQGRLHTLDDVRGPRGLLVMF
                     VCNHCPFVVHLAPALTAFARELDAQDIGVVAVNSNDIEKYPADAPDKMVEFAAAHGWS
                     FPYLLDADQSAAHAYVAACTPDFYLFDGALALAYCGQFDSSRPRNDQPITGADLRAAV
                     AAMLAGEPPLSRQLPSSGCNIKWRPGNEPEHFTKRAAT"
     gene            3852..5198
                     /locus_tag="PLP58_17665"
     CDS             3852..5198
                     /locus_tag="PLP58_17665"
                     /inference="COORDINATES: protein motif:HMM:NF013961.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase domain-containing protein"
                     /protein_id="HRJ10412.1"
                     /translation="MLSRLLVWSILAGVTLAGALLVVRSDHVSFWRQWIRQWTGGAET
                     YSEIRFQVDLVDRLNYGRISARLPLLKLDEGLENWLEEHFPQMALDDINAVSARVQEA
                     MPRYYRVAVCAVSGPSLRAILDEFQDFREQQLPEMTHLACVVRPQTGGFSRQALLVVG
                     QRLQEFNPENLTAEMEDAYFSSCLHCRHPHIVRAVKKRHSLGLECPQCRRTYAVVASD
                     ENGVFRFANEFLTGYAPPAVFSKDQSRVHELFTIWTAVHDNCRYVKDPVEGKVSTDTW
                     QTSLETQRLGQGDCEDSAIYLCDWLLTRGFQARVVLGRFGDLGGHAWVVVKLDDKEYL
                     LESTEGRPDPSNPPMASRVGSRYVPEVMFDRHAIYVRATPGQAWKGDYWSAKYWTRIE
                     PRSLLPDTEMSAPRVARTTRPDPAVAPFMELEEIPRDATEWRMNSDIGREIPAASR"
     gene            complement(5225..6295)
                     /locus_tag="PLP58_17670"
     CDS             complement(5225..6295)
                     /locus_tag="PLP58_17670"
                     /inference="COORDINATES: protein motif:HMM:NF024698.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HRJ10413.1"
                     /translation="MNEPHPLLPVLDRPYRAAEMHVFGKDRGSAFYEMAHRCAQSLWQ
                     EGLPAQAILMLNRALSSPMPGTEPVLQAWPLPYRALAWLMLNRREGQFMGNPRRHWQH
                     LATRMVEPHKDLRTWRAWACWYLAKEILPEGEFPPDLRQIREEGVVEPAHDEIAAQLA
                     RLSPADDLAVWSDTLAWSREQLGVEARRPLSARIRRIGPDELRVVQELGHEIWWSYYP
                     GIISEAQIDYMLSIWYQPDAMAREMETRGVWYALVEAEARGPVGYISFERYLESDIVF
                     INKLYLLSEVHGRGLGAAALEWTSERAREMGGTRLQLRVNKRNAPAIRAYQRAGFRFV
                     EDVCSDIGSGFFMDDFIMEKAL"
     gene            complement(6371..8170)
                     /locus_tag="PLP58_17675"
     CDS             complement(6371..8170)
                     /locus_tag="PLP58_17675"
                     /inference="COORDINATES: protein motif:HMM:NF012958.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase-fold protein"
                     /protein_id="HRJ10414.1"
                     /translation="MKRAVFFLIAASALAAEPEFPLTADSKPQEGVPKGTMLKGTHTA
                     SEGSVFPGTVRDYQIYLPAGFDKAKPAPFMVFQDGVIYQAPVVFDNLIAKKDIPPLVG
                     LFIKPGVVPAANDNALPRFNRSYEYDSITPTYSQFLIDEFLPAIEAKHGLKLSTDPNH
                     AAIAGNSSGGICAFMAAWHRSDRFRRVFTGVGTYVGIHGADQLPVLVRKFEPKPLRIF
                     LQSGTGDNNLYCGDWWMANQMMERSLTWAGYDVNHAWGEGGHNQKHASQVFPDAMRWL
                     WRGWQTDIEIQANPKGESKWKGYEVCVGDNWVKIEVEGLRSLETIKSSVFGDVIGLDH
                     NPNEIVVLANSGAVTRSRRESVTAFACGKEGAIYYSTQNLSVSKEDDVGSFKLTLEQP
                     NGTSKLLCSGTQMMVSDILVKSTGEIILLAYRSGPETHALFTVEEQKPKQIWPTSKIK
                     MSEEPYRITFSPDQSLIYARDSGSRIRSYSLMKPPRISYVQDFFILDTDSTNADRAED
                     WPNGYSPEGACVDTNGWLYVATSLGIQVCDQAGRVNFIIPTPQPAHDVCFGGKDLSEL
                     FIACGDTIYKRPTKARGIVSGQQAPIKPAPPKL"
     gene            8295..>8774
                     /locus_tag="PLP58_17680"
     CDS             8295..>8774
                     /locus_tag="PLP58_17680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ10415.1"
                     /translation="MTLTQDRHLQTGIAGSLVIHALLFIAFVWVVNSPAALQWLEQSI
                     VPEEKPPAPEVVMIFPEQIRVESPKPQQLYIRTTQNEEAGQAPANAPFQSDRNTRAAS
                     QEPPAPGATLPMPTLKGDAPNIRELANRDHRDGEIKNDSAPSTPPAPQMLPPPASAPP
                     "
BASE COUNT         1589 a         2577 c         2844 g         1764 t
ORIGIN      
        1 ggtgttcatg gcggtggagg gagattcggg attttttcgg atgctacagg atggggaggg
       61 aaaattgaac cttgaatttg gcgggtggat gcgcgccatt ttttagacgc ggcttttgat
      121 gccgttcacg gattggaaga cggcggagat gatggaatag gcgacgacgc cgacgatgac
      181 ggccatgaac aggatgatga cgggcgagat gagcgcggtc atgcgtttga tccgcttgtc
      241 cagctccttg tcgtagcggg tggcggcttt ttcgagggac ttgccgatct ctccggtctg
      301 ctcgccgatg gcgatcatgt ccacgagcat catggggaag tggccgactt ttttgagcga
      361 gttggagagg gaggagcctt cggagacgag gttgttcacc ctggcgagca gggattgaat
      421 gaagacgttg gcggagattt tggagacaag gcggatgctg ttgagcaggg gcacaccatt
      481 gccgacgaga ctgcccatgg agtgcgcaaa ttgggcgtag aagcgggtgg cgatgacggg
      541 gccgaaaagg ggcgtgtgca gcttggtctg gtgccaccac atgagtccct tgggtgtggc
      601 gatgtaggct ttgaaggaga gggcgagaat gacaactaca ctgagcatga cccaccacca
      661 ggaagcgagg aagttgttga agctgatgag catctgcgtg gcgaagggca gcttctggcc
      721 gcttttggcg agcaagtcgg tgatctgcgg caccatgacg gtcatgaaga cgatcatgag
      781 caccgcgcag gcaccgatga ggaaggcggg gtagatcatg gcctgggtga ccttggcctg
      841 tagctcggcg atgacggcca gattctgtgc caggcggcgc aggatgggcg ccagggtgcc
      901 gctgacctcg ccggcggcga cgaggttgca atacaggtcg tcaaagctgg gcgaggcttt
      961 tttcagcgcc ttcgccacgg tggcgccctc gcggatgtgc tctcgcagga tggcggagac
     1021 ctggcgcagg gcgggggcct cctggcgctc gttcatgacg cgcagggcct ggtcaatttg
     1081 caggccgcca tcgagcaggt cggcgagttc ctcggtgaag agaatgattt gagcgcgctt
     1141 gagcttcacc ggttcattcg gcgcggcctg cgaggcgctg ctggccgcgc ctgctttggc
     1201 ggcttttgcc tcctccatga ctttgaccgg cgttaatgcc tgcgcctcca gtttccgaaa
     1261 cgcttccgcg cgcgagccaa cggcgagact gccagtaatg gtctgaccgg acggattgag
     1321 ggcggtgtag gtgaaagagg gcattaggaa tggctgtgag ttattgggga tgtaatgatc
     1381 tgcggctgga gatccattag ccgggcatgg cttccctggc tgtgttcaat tcactccccc
     1441 gccgcggtga ctgtcatcac ctcttccagt gtggtgatgc cttcggcggc tttgcggaat
     1501 ccgtaatggc gcatgggaat catgccgtcc tgaagggcct gggccttcag ttcggcggtg
     1561 gtggcgcggg cattgatctt ttcctgaaga gcgccggtca tttggcatat ctccatgatg
     1621 gcaaggcggc cgctgaagcc ggtgttgcgg caggaacggc atcctcccgc ctgccagagc
     1681 tggcctttcc actccatcgg gaaacccatg gatttgaggt gtccatcttc atagtgggcg
     1741 ggctttttgc aatatgggca gagcgtgcgc acgaggcgct gggcctggaa ggcgcgcacg
     1801 gaggaggaga tcatgaatgg ctcgataccc atgtcgagca ggcgggagat gccgcccacg
     1861 gcatcgttcg tgtgcagggt ggaaaagaca agatggccgg tcagcgcgcc gcggatggcg
     1921 atctccacgg tctcctggtc gcgcatttca ccgaccatga tcacattcgg gtcgccgcgc
     1981 aggatggagc gcaggccggc ggcaaaggtg aggtcaatct ccggcttcac ggcgatctgg
     2041 acgacgccgt cgagcttgtt ttccacgggg tcctcgatgg tgacgatgcg gcggtctttg
     2101 gtgttcagct ccttgagcag ggtgtagagc gtggtgctct tgccggagcc ggtggggccg
     2161 gtgacgagga tgatgccgtt gggttgggcg agcaggcggc ggaaaatgga ctcggtctgc
     2221 gcgtcgaggc cgagctgggc catgtcgaac tttttccggg tcagcaggcg cagcgccaca
     2281 gactcgccgt tgacgctggg aatggtggcc acgcgcacgt caatggggtc gccgtcgagt
     2341 tcgaggttga tgcgcccgtc ctgaggcaga cggcgctcgg cgatgtccag atgcgccatg
     2401 atcttcaagc gggagacgag cgagttttgc agcaggcgca tgttcggcgg cacgggcacc
     2461 tcgatgagct tgccgtcaat gcggtagcgg atgcgcaggt cgcgctccag aggctcgaca
     2521 tgaatgtcgg tggctcgctc cttcagtccc tcgcggaaga tttgattcac gaagttcatc
     2581 accgtggctt cctcgtccgc ctcgtcgagc acggtggtct cctgcttcag gtcatcgtcg
     2641 tcctctatgc cttcccggcc ttccagcagt tcctcaaaat tctccgcccc cacgccatag
     2701 ccctgatgca gcgactccaa aatgcggtgc cggctggcga tctgccaatg cacgcgcttg
     2761 cccagctcct gccccaccgc ctggcgcgcg ccgaggtcga aggggttgta ggtcaaaagc
     2821 gtggcctcca cgctgctcac atgcgcgggg cagatgcggt ggcgcagggc cagctttgcc
     2881 gggaagcgcg cgtgcagtcc ctcctgttcc tcgccatttt cccgcgcaaa cggcatctgc
     2941 agtgtgccgg cgagagtttc gaaaaaagcc tcctcatcca ccagattcgc atccagcagc
     3001 gcttcgatga gcggctggcg cttgtcggcg gcgtcctcca gggtgtggcg caggcgggag
     3061 gtgtcacggc agccggcgtc tgcggcggct tggatgagaa tgtctggcag cgtggtggtg
     3121 accatgaatc tcttggaacg ccgggcgcgg gatcaagttg cggcgcgctt ggtgaaatgc
     3181 tccggctcat ttccgggacg ccacttgatg ttgcatccag agctgggcag ttggcgggaa
     3241 agcggaggct ctcccgcgag catggctgcc acggcggcgc gcaggtccgc gcctgtgatg
     3301 ggctggtcat tgcgcggtcg ggacgagtca aattgaccgc agtaggccag tgccagcgcg
     3361 ccatcgaaaa ggtaaaaatc cggcgtgcag gcagcgacat aggcgtgggc cgcgctttgg
     3421 tccgcatcca gcaggtaggg gaagctccag ccgtgcgcgg cggcgaattc aaccatcttg
     3481 tcaggcgcgt cggcggggta tttctcgatg tcgttcgagt tcacggctac cacgccgatg
     3541 tcttgcgcat ccaactcacg cgcaaaagcg gtcagcgccg gggccagatg cacgacaaag
     3601 gggcagtgat tgcacacaaa catcacgagc agcccgcgcg ggccgcgcac atcatccagg
     3661 gtgtggagcc gaccctgggc atcgggaagc tcgatttccg gggcagggga gccaagaggc
     3721 aggattcggg tggaggggac ttcggccatg tgatttttag agggttcttg atgcccgcga
     3781 aggcgactcc tggatggact tcgaggcaag ttttgctttg cctttttgtt tataatttct
     3841 ataattcaag gatgttgtcg cgtctgctcg tatggtcaat tctcgccggt gtcaccctgg
     3901 cgggcgcgtt gcttgttgtc aggagcgacc atgtcagctt ttggcggcaa tggatacggc
     3961 agtggacggg cggcgcggag acctattccg agatccgctt ccaggtggat ttggtggacc
     4021 ggctcaatta cgggcgcatc tcggcccggt tgcctttgct gaaactcgac gagggactgg
     4081 agaactggct cgaagaacat ttcccgcaaa tggctcttga tgacatcaat gccgtcagcg
     4141 cgcgcgtgca ggaagccatg ccgcgatatt accgggtggc cgtttgcgcg gtgagcggcc
     4201 ccagcttgcg cgccattttg gacgagtttc aagacttccg tgagcagcag cttcccgaga
     4261 tgactcatct ggcttgtgtg gtccgtccgc aaacaggcgg cttttcccgg caggcgctgc
     4321 ttgtcgttgg ccagagactg caagagttca atcccgaaaa cctcaccgct gagatggaag
     4381 acgcctactt cagctcatgc ctgcactgcc ggcatccgca catcgtccgc gcggtcaaaa
     4441 aacgccacag cctgggtctg gagtgccctc aatgccggcg cacctatgcg gtggtggcct
     4501 cggatgagaa tggcgtcttt cgctttgcca acgagttcct taccggttac gcgccccccg
     4561 ccgttttctc caaggaccag tcccgtgtgc atgagctttt caccatctgg actgccgtgc
     4621 atgacaactg ccgttatgtg aaggatcccg tcgagggaaa agtatccacc gacacctggc
     4681 agaccagtct ggaaacacag aggctcggcc agggagactg cgaggactcg gccatttatc
     4741 tttgtgactg gcttctgacg cgcgggttcc aggccagggt cgtgctcgga cgcttcggcg
     4801 acctcggcgg ccatgcttgg gtggtggtga agctcgacga caaggaatat ctgctcgagt
     4861 ccaccgaggg ccgtcctgat ccctccaatc cacccatggc ttcgcgggtt ggcagccgct
     4921 atgtgccgga ggtcatgttt gatcgccacg cgatctatgt gcgcgccaca cccggccagg
     4981 catggaaggg cgactactgg tccgcgaaat actggacgcg catcgagccg cgctcgcttt
     5041 tgccggacac tgagatgtca gcgccaaggg tggcgcgcac cacgcggcct gatccggcgg
     5101 tggcgccctt catggagctg gaggaaatcc cgcgcgatgc caccgagtgg cgcatgaact
     5161 ccgacatcgg gcgcgaaatc cccgccgcct cccgctgatc tggaacgcgc gcatggcacg
     5221 agcgtcacag cgccttttcc atgatgaagt catccatgaa aaagccgctg ccgatgtccg
     5281 agcacacgtc ctcgacaaag cggaagcccg cgcgctggta ggcgcggatg gcgggcgcgt
     5341 tgcgcttgtt cacgcgcagt tgcagccgtg tgccgcccat ttcgcgggcg cgctcactgg
     5401 tccattccag agccgccgcg cccaggccgc gcccgtggac ctcggacaga aggtagagct
     5461 tgttgatgaa gacgatgtcg ctctccagat agcgctcgaa ggaaatgtaa cccaccggcc
     5521 cgcgcgcctc cgcctccacc agcgcatacc acacaccgcg cgtctccatt tcccgcgcca
     5581 tggcgtcggg ctggtaccag atgctcagca tgtagtctat ctgcgcctcg ctgatgatgc
     5641 cgggataata cgaccaccag atttcatgcc ccagctcctg caccacgcgc agctcatccg
     5701 gtccgatgcg gcggatgcgc gccgagagcg gccgccgcgc ctccacgccg agttgctcgc
     5761 ggctccaggc cagcgtgtcg ctccacaccg ccagatcatc cgccggggaa aggcgcgcga
     5821 gttgtgcggc gatctcgtca tgcgcgggct ccaccacgcc ctcctcgcgg atctggcgca
     5881 ggtccggcgg gaactcgccc tcgggcagga tctccttcgc cagataccag caggcccagg
     5941 cgcgccaggt gcgcagatcc ttgtgcggct ccaccatgcg cgttgccagg tgctgccaat
     6001 gccgccgtgg attgcccatg aattgcccct cccgccggtt cagcatcagc caggccagcg
     6061 cgcgatacgg cagcggccat gcctggagca ccggctccgt gcccggcatg ggcgaggaaa
     6121 gcgcgcggtt cagcatcagg atcgcctgcg ccggcagccc ctcctgccac aggctctgcg
     6181 cgcaacgatg cgccatctca taaaaggccg acccgcggtc cttgccaaaa acatgcatct
     6241 ccgccgcgcg atacgggcgg tccaaaacag gcagcaaggg gtggggttcg ttcatggcgc
     6301 catcatcttc atccgtctct taatccttgt tttaatccat ccagcaaagc tcgtcccagc
     6361 cattctaaac tcacagtttc ggtggagccg gcttgattgg tgcctgctga ccactgacga
     6421 tgcctctggc cttcgtcggg cgtttgtaga tcgtgtctcc gcaggcgatg aagagctcgc
     6481 tgaggtcctt gcctccgaag cagacatcat gcgctggttg cggtgtggga atgatgaagt
     6541 tcacgcgtcc ggcctgatcg cagacctgga tgccgaggct ggtggcgacg tagagccagc
     6601 cgttggtgtc cacgcaggct ccttcaggcg aatatccatt tggccaatcc tctgccctat
     6661 ccgcgttggt ggagtcggta tccaagatga agaaatcctg aacgtaacta atccgaggtg
     6721 gcttcatgag tgaatagcta cgaattcgag atccgctgtc ccgggcataa atgagcgact
     6781 gatcagggga aaaagtgata cggtatggct cctctgacat ctttatcttt gaagtaggcc
     6841 aaatctgctt tggtttttgt tcttccacag tgaagagcgc atgagtttct gggccagagc
     6901 gatatgccaa gagtattatt tcccccgtag acttaaccaa tatgtcgctc accatcattt
     6961 gagtccctga acagagcagc ttggaggtac cgttgggttg ctccagggta agcttgaaac
     7021 tgccaacgtc gtcttccttg gatactgaca aattttgtgt gctgtagtag atggcacctt
     7081 cctttccgca tgcgaaggca gtgacacttt ctcgtctcga acgagtcacc gctccggagt
     7141 tggcaagcac gacaatctca ttggggttgt ggtccagccc tattacgtcg ccaaataccg
     7201 aggatttgat ggtttcaaga cttcgaagtc cttcaacttc gattttgacc cagttgtcac
     7261 cgacacacac ctcatacccc ttccacttcg actcgccctt cggattcgct tggatctcga
     7321 tgtccgtctg ccagccgcgc cagagccagc gcatggcgtc ggggaagacc tggctggcgt
     7381 gcttttggtt gtggccgcct tcgccccagg cgtggttcac atcatagccg gcccaggtga
     7441 ggctgcgttc catcatctgg ttggccatcc accagtcgcc acagtagaga ttgttatcgc
     7501 cggtgccgct ttgcaggaag atacggaggg gcttgggctc gaatttgcgg acgaggacgg
     7561 ggagctgatc agcgccgtgg atgccgacat aggtgcccac accggtgaag acgcggcgga
     7621 agcggtcgga ccggtgccag gcggccatga aggcgcagat gccgccgctg ctgttgccgg
     7681 cgatggcggc gtggttggga tcggtgctca gtttgaggcc atgcttcgcc tcgatggcag
     7741 gcaggaattc gtcgatgagg aactgcgagt aggtgggcgt gatgctgtca tactcatagc
     7801 tgcggttgaa gcgcggcagg gcgttgtcgt tcgcggcggg taccacgccc ggcttgatga
     7861 agaggccgac cagcggcggg atgtcctttt tggcgatgag attgtcaaag acgaccggcg
     7921 cttgataaat gacaccgtcc tggaagacca tgaaaggcgc gggcttggcc ttgtcgaagc
     7981 ccgcaggcag gtagatttga taatcgcgca ccgtgccggg gaagacgctg ccttcgctcg
     8041 cggtatgggt gcctttgagc atcgtgccct tgggcactcc ctcctgcggc ttcgagtcgg
     8101 cggtgagggg aaactccggc tccgcggcaa gggcagaggc ggcgatgagg aaaaagacag
     8161 cgcgtttcat gccagtgatg gtgcgcggga cccgccgcgc tggcaagagg cgaaggcggc
     8221 gccagccgtc ccttcgtcag cagagcgcag gatgttgttt gcttccccgc cgccgggagg
     8281 catcttccag gcttgtgact ctcactcaag acaggcatct gcagaccggc atcgcgggat
     8341 cattggtgat ccacgcgctg ctgttcatcg ctttcgtctg ggtggtgaac agccccgccg
     8401 cgctgcaatg gctggagcag agcatcgtgc cggaggaaaa gccgcccgcg cccgaggtgg
     8461 tgatgatctt tcccgagcag atccgcgtcg agtcgccgaa gccgcagcag ctttacatcc
     8521 gcaccaccca gaacgaggag gccggacaag cgcccgcaaa cgcccccttc cagtccgacc
     8581 gcaacacccg cgccgccagc caggagccgc ccgcgccagg cgccaccctg cccatgccca
     8641 ctctcaaagg cgacgcgccc aacatccgcg aactcgccaa ccgcgaccac cgcgacggcg
     8701 agatcaaaaa cgactccgcc ccctccaccc cgcccgcgcc gcagatgctg ccgcctcccg
     8761 cgtccgcgcc accg
//
LOCUS       DAOMYJ010000212         8770 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Prosthecobacter sp. isolate AMR_MDS_5629
            NODE_2656_length_8770_cov_19.960184, whole genome shotgun sequence.
ACCESSION   DAOMYJ010000212 DAOMYJ010000000
VERSION     DAOMYJ010000212.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867850
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prosthecobacter sp. (wastewater metagenome)
  ORGANISM  Prosthecobacter sp.
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Verrucomicrobiaceae; Prosthecobacter.
REFERENCE   1  (bases 1 to 8770)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8770)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.30
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:34:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,742
            CDSs (total)                      :: 4,684
            Genes (coding)                    :: 4,676
            CDSs (with protein)               :: 4,676
            Genes (RNA)                       :: 58
            tRNAs                             :: 55
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8770
                     /organism="Prosthecobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2656_length_8770_cov_19.960184"
                     /isolate="AMR_MDS_5629"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1965333"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1622
                     /locus_tag="PLP58_17685"
     CDS             <1..1622
                     /locus_tag="PLP58_17685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ10416.1"
                     /translation="AMASAQVTGGPLPRVPALDAIPEALELLAAVKTDEKKILPWPGW
                     RTAWLEDRWLKQHDFFKDAAAIAEKSRAAFTAWTTRLMDVMLALSVSALLLTLSWRSD
                     LWLRVLGEDKLYALLGLGGAALALGLLLLQVLRSMQEVNRRVGRFTRQKVMLEKARAE
                     LEAAADDEPARRIVRETEAQLLGEVADWHFETENSERFFQIREQRGDRAVAGPAAAPA
                     RAWGPLLWLLAAGGAGLAFLLRVVLGRAPWVAGSCALTLGCLGLIMPKDPVSRQRLEA
                     VGNVLGVDRKAWNPAPARAQNGCVIIAHGLRDGAFFTARGEESRWMSDMAMNLAQRLD
                     PAQPNIGLVDWSSAAMPSQWHGLLMKQENLDGLSMGLDLALIRDQAREVGDHLGFRLA
                     QWIRDKDQRIRRDKPLHLIGHSAGGFLVARAALVLSELGLAPETMHVTILDTPGTDDA
                     LFAETGRVCRMEFYKTSDFVTSLDVERMPEDVFFRVIPPPGKPSLREAHSYAWEWYSA
                     TICECAIGDEGFGRSPFLLTRAAPQPDTEGR"
     gene            1658..3106
                     /locus_tag="PLP58_17690"
     CDS             1658..3106
                     /locus_tag="PLP58_17690"
                     /inference="COORDINATES: protein motif:HMM:NF014124.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RyR domain-containing protein"
                     /protein_id="HRJ10417.1"
                     /translation="MPSYTPSPINTDNVELPPEITALSERLAESIHDVWALERIAQGW
                     KHGPKRDDALKEHPCLVPYDALSESEKVFDRKTASASLKSVLALGYEIKRKPAVIEPG
                     EQRDLTAWQQALEAEAGKANKNGARGWTFDMDDGPEIASLPPGGMNVAREVLRELQDR
                     VFPVWQAEDATALQKQARHARIAAFAIWPGILAVVCAILQLSFHHFGQAWRGVAEMFL
                     MLEFALVMAAVAAVLIGLLSNVHHGWLAHRQRAERLRGLKFRALSWPELWCDFERWKA
                     RLAHEVGELRAVTTKAAHHWAREKDTVHPELPEVPACVVPEADLKALSALYRVKRLEF
                     QHHYFGRQSVKADRSSWVVNTKLGLVLFFLSVVFVLMHAGHHYLHEAPAGGAAQGHGL
                     PLFDVVTISLAALLPVIGFGFRAWLAAFEAPRSRNLYRAKALALEDYMKRSAEAAGDV
                     GQALAHIAQGEHFFINEHREWCRLQMEAEWFV"
     gene            3137..4531
                     /locus_tag="PLP58_17695"
     CDS             3137..4531
                     /locus_tag="PLP58_17695"
                     /inference="COORDINATES: protein motif:HMM:NF025056.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003953 - NAD+ nucleosidase activity
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="toll/interleukin-1 receptor domain-containing
                     protein"
                     /protein_id="HRJ10418.1"
                     /translation="MSASFRNRSHQCFVSYSHQDAGFAGRLVVWLDAAGIPVWLDETS
                     LGVGEKVARALPDEMSQCRGMLLLASRRSMESNWVRQEINAALLENVENPAFAITIIR
                     LDDVDIASHVSALRQFKWIEAGTNVSVFEPGFLVDVVNSLHRRAPSPILNHQVDVYVS
                     RSDRNAAEIKRSDAACRRLSAHHGIHLIGDAVDQADFSEERIRILMGTCSSHLLVLPA
                     RAKEADYKYFLRERAISESLNLPCLVCAEPGSPLPDGLRNGDHFIELQTGGDDILGES
                     GREVLEAFLDDTRATTARHSASIFFAHEYNQNQERNRAARNLLASVTGLTPHAGSDFT
                     SAIGPRQILDGISRSAWFVADLGSKTNPETGRLEVNVNTCIEVGIALGAGLGKVCSTA
                     PGARAERPVYALSFDRTAGQAGAAGETGKTRDIPWMLRSGITIQWYASDTDFLAIVHR
                     IARGHRRRLLNAEL"
     gene            complement(4607..5659)
                     /locus_tag="PLP58_17700"
     CDS             complement(4607..5659)
                     /locus_tag="PLP58_17700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ10419.1"
                     /translation="MAWSKRGKIWGRERESGDAAPWIHETSVDEALEAVRRQWKEHGI
                     HAENGDWPLIQLAHIAVMRGLTRRVAPLVARLLRRREAEGKLRWRLEHDRAGAVGISM
                     VRLMAAHVEDPALPVSQARADTALFLRSIEACDDLRSVHQLMPLITATRLDAGTAQPL
                     KKGGKLPLRAEMASTGICVAAHRQWHVEYLLAFGAEDEALELAHKGRSDQPCGDTCAF
                     APHSMYAWLLEPLRRRGRADEARVLHDRLESLMIPRLLYLNAMGHRIHYLALEDRFED
                     AGHLIRQMLPLAEEPDASPWQKLKFYEGCAKALQTARSKKAGASRILGPQARETSARI
                     RAGSEACRQLFDQRPA"
     gene            complement(5675..7504)
                     /locus_tag="PLP58_17705"
     CDS             complement(5675..7504)
                     /locus_tag="PLP58_17705"
                     /inference="COORDINATES: protein motif:HMM:NF024977.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HRJ10420.1"
                     /translation="MQSKTPDAIAACDSAMQQNSTPPPVTENFNVHLGGTLSILSEAI
                     YTAGPEVFVRELMQNGCDAITGRVLADPGFMDGAVSLDVVTGRDSSLTLIAEDNGIGL
                     TLEESRRALSTIAFSSKRPDQGGADDSPYVGRFGIGLLSGFLVADEITVISRRAGGGH
                     PAVHWTGRIDGTFTTLPVDGVTSTGTRVFLRLRPEAAREFTAEAVFEIAQKYGRYLPH
                     PVTFRHDGNARLVTDEQPIWEREMDMADLLELGHEIFEVPMLSAFPFESSEAGARGIA
                     FIQAEPCHATAESTHVIFIKNMLVSERALDLEPPKAPFLRLIMNSDRLRPNAGRDAVM
                     AGDPRLPALRGDIEAAFKEHLARLHREQPALCAAIVTLQYRCLGELAARDHAYLRHLV
                     EHLPVETTLGRMTLGQIFKRHAGTLEYVTETTEFQRVQARARAEGGCIARVETEPAHR
                     LMELVGKASKGMKVRRITASEYLARFTKSAGAPSQREQLMLELAEEELRPENCRGVFY
                     DTDEPDEITRLDMGADESLERLLSVDSGDGSAAKKLLLNRGHPLISQMIDGAADAALL
                     RVWIRVLYQFALLEAREVPNAAESRRFSRAIGNAFTASSLGNL"
     gene            7727..>8770
                     /locus_tag="PLP58_17710"
     CDS             7727..>8770
                     /locus_tag="PLP58_17710"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HRJ10421.1"
                     /translation="MISAPEQTYLSTCPVCQNQIDVTALEPFTKLKCPFCGQMVRVRR
                     KFDHFIIQRQIGEGGMSRVFEAEDETLGRRVALKILNPQYGRDAARVEQFRQEALITA
                     NVNHPNVIKLYSVGYDQGYFYIAMELVTGGSLEQRIRKEGAIKEEEALRIGREVAEGL
                     RAAQQQGLIHRDVKPANILFTETGTAKVVDFGLALFVERGPDASGEIWATPYYVAPEK
                     VIENREDYRSDIFSLGASLYHALTGRPPHKVDSNDIAELRRVKCRRVILEDSGVRFAP
                     RTAHVVNRMLAFRPQERCANYDETVEELRLAEGLVGQSGYRLRFATRRARWLAVAAAV
                     LALAFGIGWFARST"
BASE COUNT         1477 a         2629 c         3052 g         1612 t
ORIGIN      
        1 gggcgatggc atccgcgcag gtgacgggcg ggccgctgcc gcgcgtgccg gccctggatg
       61 cgatcccgga ggcgctggag cttctggccg cggtcaagac cgacgagaaa aaaatcctgc
      121 cctggccggg gtggcggacg gcctggctgg aggatcgctg gctgaagcag catgattttt
      181 tcaaagatgc cgccgccatt gcggagaagt cccgcgcggc attcacggca tggaccacgc
      241 gcctcatgga tgtgatgctg gctctgtccg tgtcagccct gctgctcact ctgagctggc
      301 gttctgatct gtggctccgc gtgctgggag aggacaagct ctacgcgctg ctcggcctgg
      361 gcggagcggc gctggccctg ggcctgctgc tgctgcaagt gctgcgctcc atgcaggagg
      421 tgaaccgccg cgtggggcgc ttcacccgcc agaaagtgat gctggaaaaa gcccgcgcgg
      481 aactggaggc ggccgcggat gatgaaccgg cgcggcgcat cgtgcgggag accgaggcgc
      541 aactgctcgg cgaggtggcg gactggcact ttgagactga gaattccgag cgctttttcc
      601 aaatccgcga gcagcgcggc gaccgtgcgg tcgcaggacc ggcggccgct cccgccaggg
      661 catggggacc gctgctctgg ctgctggccg ctggcggtgc ggggctggcg tttctgctgc
      721 gcgtggtgct gggccgcgcg ccctgggtgg cgggatcctg cgcgctgacg ctgggctgcc
      781 tgggcctgat catgcccaag gaccccgtct cacggcagcg gctggaggcg gtgggcaatg
      841 tgctcggtgt ggacaggaag gcgtggaacc ccgcgcccgc ccgcgcgcaa aacggctgcg
      901 tcatcatcgc ccacggcctg cgcgacggcg cttttttcac cgcacgggga gaggagagcc
      961 ggtggatgag cgacatggcg atgaacctgg cgcagcgtct cgacccggcg cagccaaaca
     1021 tcggcctggt ggactggtcg tccgcggcca tgccctcgca gtggcacggg ctgctcatga
     1081 agcaggaaaa tctggacggc ttgagcatgg gcctggatct ggcattgatc cgcgaccagg
     1141 cgcgcgaggt gggggaccat cttggatttc gtcttgctca atggatacgg gacaaggacc
     1201 agcgcatccg gcgcgacaag cctctgcacc tcatcggcca cagcgcgggc ggtttcctgg
     1261 tggcgcgggc ggctctcgtg ctcagcgagc tgggactcgc cccggagacg atgcatgtca
     1321 cgatcctaga cacgccgggg acggatgatg cactgtttgc ggagacgggc cgcgtttgcc
     1381 ggatggagtt ttataaaact tcggacttcg tgacaagcct cgatgtggag cggatgcctg
     1441 aggacgtgtt tttccgcgtc atcccgccgc cgggtaagcc ttcactgcgc gaggcgcact
     1501 cttatgcgtg ggaatggtac tccgccacga tctgcgagtg cgccattggt gacgagggct
     1561 tcgggcgctc tccattcttg ctgacccgag ccgcgccgca gcccgacacg gaggggcgtt
     1621 gattcaggac gcgcacgcct ctaccatcct gaaattcatg ccctcctaca cacccagccc
     1681 gatcaacacg gacaacgtcg aactcccgcc ggagatcacc gcgctctcgg agcggctggc
     1741 ggagagcatt cacgatgtct gggcgctgga acgcatcgct caggggtgga agcacggccc
     1801 caagcgtgat gacgcgctca aggagcatcc ctgcctggtg ccttatgacg ctctgtctga
     1861 gagcgagaag gttttcgacc gcaaaaccgc ctcggcttcg ctgaagtcgg tgctcgctct
     1921 cggatatgag atcaagcgca agcccgcggt catcgagcca ggggagcaga gggatctgac
     1981 ggcctggcag caagcgctgg aagctgaagc gggcaaagca aacaagaacg gcgcgcgcgg
     2041 gtggaccttc gacatggatg acggcccgga aatcgcgagc ctgccgcccg gcggcatgaa
     2101 tgtggcgcgg gaggtgctgc gagaactcca ggaccgcgtt ttccccgtct ggcaggcgga
     2161 ggacgccacc gcgctgcaaa agcaggcccg ccacgcgcgc atcgccgcct ttgccatctg
     2221 gcctggcatc ctggctgtgg tgtgcgccat tctccagctt tcgttccatc attttggaca
     2281 ggcgtggcgc ggggtggcgg agatgttttt gatgctggag ttcgcgctcg tcatggctgc
     2341 ggtggcggcg gtgctcatcg ggcttttgtc aaacgtccac cacgggtggc ttgcgcaccg
     2401 ccagcgggcc gagaggctgc gaggattgaa attccgcgcg ctttcctggc cggagttgtg
     2461 gtgtgacttt gagcggtgga aggcgcgcct cgcacacgag gtgggggaac tgcgcgccgt
     2521 cacgaccaag gcggcgcatc attgggcgcg ggagaaggac acggtgcatc cggaactgcc
     2581 ggaggtgcct gcctgtgtcg tgcccgaggc ggacctcaaa gcgctctccg ccttgtaccg
     2641 ggtgaagcgg ctggagttcc agcaccacta tttcgggcgt caatccgtga aggcggaccg
     2701 cagttcctgg gtggtgaaca ccaagcttgg cctggtcttg ttcttcctct ccgtcgtgtt
     2761 cgtgctcatg cacgcggggc atcattacct gcatgaggcg cccgccggcg gggcggctca
     2821 aggccatggt ctgccgttgt tcgatgtggt gaccatcagc ctggcggcgc tgctgccggt
     2881 catcggcttt ggattccggg catggctggc ggctttcgag gcgccgcgca gccgcaatct
     2941 ctaccgcgcg aaggccctgg ctttggagga ttacatgaag cgcagcgcgg aggcggccgg
     3001 cgatgtcgga caggcgctgg cgcacatcgc gcagggcgag catttcttca tcaacgagca
     3061 tcgcgagtgg tgccgtctgc agatggaggc ggagtggttt gtgtgagatg agccggcagg
     3121 gcagtccttt cattttatga gcgccagctt tcgcaaccgc agccatcagt gttttgtgtc
     3181 ttacagccac caggacgcgg gatttgccgg caggctggtt gtgtggctgg acgcggcggg
     3241 catccccgtg tggctggatg aaaccagcct gggggtgggt gaaaaagtgg cgcgcgccct
     3301 gcctgacgaa atgtcacagt gtcgcggcat gctgctgctc gcctcccggc gttcgatgga
     3361 gtccaactgg gtgaggcagg aaattaacgc ggcgttgctg gagaatgtgg agaaccccgc
     3421 gtttgccatc accatcatac gcctcgatga cgtggacatc gcctcgcatg tgtccgcgct
     3481 gcgccagttt aagtggattg aggcggggac aaacgtttcg gtgtttgaac cgggttttct
     3541 ggtggatgtg gtcaactctc tccaccggcg cgcgccctcc ccgatcttga atcaccaggt
     3601 ggatgtctat gtgagccgca gtgaccgcaa tgccgcggaa atcaagcgct ccgacgcggc
     3661 gtgccgccgc ctcagcgcgc atcatggcat tcatttgatc ggggacgcgg tggaccaggc
     3721 cgactttagc gaggagcgca tccgcatcct gatgggcacc tgctccagcc atcttttggt
     3781 tctgccagcg cgcgccaaag aggcggacta caagtatttt ctccgggaac gcgccatcag
     3841 cgaatccctg aaccttccct gcctcgtctg tgcggaaccc ggcagcccgc ttcccgatgg
     3901 actgcgaaat ggggatcatt tcatcgagct gcaaacgggc ggtgacgaca tcctgggcga
     3961 gtccggacgc gaggtgctgg aggcgtttct ggatgacacg cgcgccacga cggcacggca
     4021 ttccgcctcc attttcttcg cgcatgagta caatcaaaac caggagagga atcgcgcggc
     4081 gcggaacctg ctggcgagtg tgaccggcct gactcctcat gcagggtcgg atttcaccag
     4141 cgccatcggt ccgcggcaaa tactcgacgg aatcagccgt tccgcgtggt ttgtcgctga
     4201 tctgggcagc aaaaccaatc cagagaccgg caggctggaa gtcaatgtga acacctgcat
     4261 tgaagttggc atcgctcttg gcgcgggctt ggggaaagtt tgttcaaccg cgccgggagc
     4321 gagggcggag agaccggtgt atgctttgtc ctttgatcgc accgcagggc aggcgggcgc
     4381 tgcgggcgaa accggcaaga cgcgtgacat tccctggatg ctgcgctccg gaatcaccat
     4441 ccagtggtat gccagtgaca cggattttct ggccattgtg catcgcatcg cccgcgggca
     4501 caggcgccgg ctgctcaatg ccgagttgtg acggaatgcc cggcattcac ggctttcccg
     4561 gaggtcgtcc ttccgcaagt cggtgctgcc aacgatgcga ggattttcat gcggggcgct
     4621 ggtcgaagag ttggcggcag gcttccgatc cggcgcgaat ccgcgccgag gtctcgcgtg
     4681 cctgcgggcc gagaatcctg ctggcgcctg cttttttcga gcgcgcggtt tgcagcgcct
     4741 tcgcgcaccc ctcgtaaaat ttcagcttct gccacgggct ggcgtccggc tcctccgcca
     4801 ggggcagcat ttgccggatc aaatgacccg cgtcctcgaa gcggtcttcg agggccaggt
     4861 aatggatgcg gtgtcccatg gcattgagat aaagcaggcg cgggatcatt agggactcaa
     4921 ggcggtcatg cagcacgcgg gcttcgtcag cccggccgcg gcgtcgcagc ggctccagca
     4981 gccaggcgta catcgagtgc ggggcgaagg cgcaagtgtc gccgcaaggc tggtcggagc
     5041 ggcctttgtg ggccagttcc agggcttcat cctcggcccc gaaggcgagc aggtattcga
     5101 catgccattg ccggtgcgcg gccacgcaga tgccggtgga ggccatctcg gcgcgcaggg
     5161 gcagcttgcc gccctttttc aacggctgcg cggtgccggc gtccagtctt gtggcggtga
     5221 tcaagggcat gagctgatgc acggaacgca gatcatcgca tgcctcgatg ctgcgcagaa
     5281 aaagcgccgt gtcggcgcgc gcctggctga cgggaagggc gggatcctcc acatgggcgg
     5341 ccatgaggcg caccatcgag atgcccactg cgccggcgcg gtcgtgctcc agccgccagc
     5401 gcagttttcc ctccgcctcg cgccggcgca ggaggcgcgc gaccagcggc gcgacgcggc
     5461 gtgtgagtcc gcgcatgacg gcgatgtggg cgagctggat gagcggccag tcgccgtttt
     5521 cggcgtggat gccgtgctct ttccactgcc tgcgcacggc ttccagggcc tcatccacgg
     5581 aggtctcgtg gatccacggc gcggcgtcgc cggactctcg ttcgcgcccc cagatttttc
     5641 cgcgttttga ccaggccatg atgggtgagc ggggtcaaag gttgccgaga ctggaggcgg
     5701 tgaaggcgtt gccgatggcg cggctaaagc ggcgggactc ggcggcgttg ggcacctcgc
     5761 gcgcttcgag gagcgcgaat tgatagagaa cgcgaatcca cacgcgaagc agcgcggcat
     5821 ccgcggcgcc gtcaatcatc tgggaaatga gcggatggcc gcggttgagc aggagttttt
     5881 tcgcggcgga gccgtccccg gagtccaccg agaggagccg ctcaaggctt tcgtccgcgc
     5941 ccatgtcaag gcgggtgatc tcatccggct cgtcggtgtc ataaaaaacg ccccggcagt
     6001 tttccgggcg caactcctcc tccgcgagtt cgagcatgag ctgctcgcgc tgggagggcg
     6061 cgccggcgga tttagtgaag cgcgccagat attcggaggc ggtgatgcgg cggactttca
     6121 tgcccttcga tgctttgccg accagctcca tgaggcggtg cgcgggctcg gtctccacgc
     6181 gcgcgatgca gccgccctcg gcgcgcgccc tggcctgcac acgctggaac tcggtggtct
     6241 ctgtcacata ctccagcgtg ccggcgtggc gtttgaaaat ctggcccagc gtcatgcggc
     6301 ccagcgtggt ctccacgggc aggtgttcca cgagatggcg caggtaggcg tggtccctgg
     6361 ctgccagctc gccgaggcag cggtattgca gcgtcacaat cgcggcgcaa agggcgggtt
     6421 gctcgcggtg gaggcgtgcc agatgctcct tgaaggccgc ttcaatgtca ccgcgcaggg
     6481 cgggcaggcg ggggtcgccg gccatgacgg cgtcgcgtcc ggcgttggga cggaggcggt
     6541 cggagttcat gatgaggcgc agaaatggcg cctttggcgg ttcgaggtcc agggcgcgtt
     6601 ctgagacgag catgtttttg atgaagatga catgggtgga ctccgccgtg gcgtggcagg
     6661 gctccgcctg gatgaaggcg atgcctctgg ctccggcctc ggagctttcg aaagggaagg
     6721 cggaaagcat cggcacctcg aagatctcat ggcccagctc cagcagatcc gccatgtcca
     6781 tttcgcgctc ccagatcggc tgctcatcgg tgacgagccg cgcatttccg tcgtggcgga
     6841 aggtcaccgg gtgcggcaga tagcgcccgt atttttgcgc gatttcaaaa acggcctccg
     6901 ccgtgaactc gcgcgccgcc tccgggcgca gccgcaggaa gacacgggtg cctgtcgagg
     6961 tcacaccatc caccggcagc gtggtgaagg tgccgtcaat gcggcccgtc cagtggactg
     7021 cgggatgccc cccgcctgcg cggcgggaga tgacggtgat ctcatcggcg acgagaaagc
     7081 cgctgagcag tccgatgcca aatcgcccca catagggcga gtcgtcggcg ccgccctggt
     7141 ccgggcgttt cgagctgaag gcgatggtgg acagcgctcg gcgggactcc tccagggtga
     7201 ggccgatgcc attgtcctcc gcgatgaggg tcagcgagct gtcccgcccg gtgacgacat
     7261 cgagcgagac cgcgccatcc atgaagccgg gatcggcgag aacccgtccc gtgatggcgt
     7321 cgcagccgtt ctgcatcaac tcgcggacaa acacctcggg gccggcggtg tagatggcct
     7381 cggagagaat ggagagcgtg ccgccgagat ggacattgaa gttttccgtg actggcggcg
     7441 gggtggaatt ttgctgcatg gcggagtcgc acgcagcgat ggcatcaggg gttttggatt
     7501 gcatggcggc gagtttgaca ccttggattt cgctggcgat tccaaaaacg agcgaaattt
     7561 ttccgctgcg cgcggggcat ggagcgaact ctggtgtcag cttccttggc gtggggaggc
     7621 tctgccatcc tgcctggggg gcattttcag cattcgaaca aataaggaaa tccgtaattc
     7681 ctggcatttt gaatgcgatg cgatagagac ctgtcccaag acccctgtga tttcagctcc
     7741 cgaacaaacc tacctcagca cctgtcccgt ctgtcaaaac cagattgatg tgacagctct
     7801 ggagccgttc acaaagctga agtgcccatt ctgcgggcag atggtgcggg tgcggcgcaa
     7861 gttcgaccac ttcatcatcc agcgccagat cggcgagggc ggcatgagcc gcgtgtttga
     7921 ggcggaggac gagacgctgg gccggcgggt ggcattgaaa atcctcaacc cgcaatacgg
     7981 ccgcgatgcc gcgcgggtgg agcagttccg ccaggaggcg ctgatcacgg cgaatgtgaa
     8041 ccatccgaat gtgatcaagc tctactcggt gggctatgac cagggctatt tttacatcgc
     8101 catggagctg gtcacgggcg gcagcctgga gcagcgcatt cgcaaggagg gcgccatcaa
     8161 ggaggaagag gcgctgcgca tcgggcgcga ggtggcggag gggctgcgcg cggcccagca
     8221 gcaggggctg atccaccggg atgtgaagcc ggcgaacatt cttttcaccg agacgggcac
     8281 ggcaaaggtg gtggacttcg gcctggcgct ctttgtcgag cgcggtcccg acgccagcgg
     8341 tgaaatctgg gccacgccat attatgtcgc cccggagaag gtcatcgaaa accgcgagga
     8401 ttaccgcagc gacatcttca gccttggcgc cagcctttac cacgcgctca cgggacgtcc
     8461 accgcacaag gtggacagca acgacatcgc cgagctgcgc cgggtgaaat gccgccgcgt
     8521 gatcctggag gacagcggtg tgcgcttcgc cccgcgcacg gcgcatgtgg tgaaccgcat
     8581 gctggccttc cgcccccagg agcgctgcgc gaactatgac gagacggtgg aggagctgcg
     8641 ccttgccgag gggttggtgg gccagtccgg ctatcgtctt cgcttcgcca cgcggcgcgc
     8701 gcgctggctg gctgtggcgg cggcggtgct ggcgctggct tttggcatcg gctggttcgc
     8761 gcgcagcacg
//
LOCUS       DAOMYJ010000213         8765 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Prosthecobacter sp. isolate AMR_MDS_5629
            NODE_2660_length_8765_cov_21.222503, whole genome shotgun sequence.
ACCESSION   DAOMYJ010000213 DAOMYJ010000000
VERSION     DAOMYJ010000213.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867850
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prosthecobacter sp. (wastewater metagenome)
  ORGANISM  Prosthecobacter sp.
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Verrucomicrobiaceae; Prosthecobacter.
REFERENCE   1  (bases 1 to 8765)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8765)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.30
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:34:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,742
            CDSs (total)                      :: 4,684
            Genes (coding)                    :: 4,676
            CDSs (with protein)               :: 4,676
            Genes (RNA)                       :: 58
            tRNAs                             :: 55
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8765
                     /organism="Prosthecobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2660_length_8765_cov_21.222503"
                     /isolate="AMR_MDS_5629"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1965333"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..574
                     /locus_tag="PLP58_17715"
     CDS             <1..574
                     /locus_tag="PLP58_17715"
                     /inference="COORDINATES: protein motif:HMM:NF014788.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="AIR synthase-related protein"
                     /protein_id="HRJ10422.1"
                     /translation="HPHVDRVHGRGDRRDAVSIVGGETSRGPALIVTVSMTGEAPAGR
                     WIPRNAARAGDLLLVTGRLGGSIRGRHLRFEPRLAEARWLARHLPVHAMMDLSDGLAK
                     DLPRMAAAAGLGFEVEMQSLPRARGVTTEQAWGDGEDYELLLAVSPRVKPAALAAWRG
                     AFPRLPLTRIGTLTRKPSTSLAPGGWDHFG"
     gene            complement(599..3109)
                     /locus_tag="PLP58_17720"
     CDS             complement(599..3109)
                     /locus_tag="PLP58_17720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009960726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PSD1 and planctomycete cytochrome C
                     domain-containing protein"
                     /protein_id="HRJ10423.1"
                     /translation="MDSHAFQTTASIFALLLAAATPTVADEIRFSRDILPVLSDTCFA
                     CHGPDEAGRKGGLRLDLQESALGRGKSGATAVVPGDLSKSEVIARILSTDPDDAMPPL
                     KALRKLSAEQKKLIQDWVAQGAPWGKHWAYEPPVRPAVPEIRSSKFEILNPIDAFIAE
                     RLQKEELTFSPDASPETLARRLSLDLTGLPPSAHDFEIRTSNFDLNSIVDRLLASPRF
                     GERWAWDWLDAARYADTNGYQGDPERTMWPWRDWVVDAINANMPYDQFTIEQIAGDLL
                     PGAARSQQLASGFHRNHMHNGEGGRIAEETRVENVFDRVETTATLWLAATFNCCRCHD
                     HKYDPLPQRDYYALYDIFNQMSETGAGRGGQAAPVLDFSTPAETGRVEAARQKLAAII
                     KEVEAFELKKFPRPEGAPLEQSDASKLPGNLPATLAKTPPERRGVDAILEAVGYFETD
                     GADPAYVAVLKKHLAAVRARDSAQSSVTKVMIMDHLKEPRDTFILDKGAYDKPTAVKV
                     AGAVPAAFAGGVALPDRASRLDLARWLVARENPLTARVTVNRYWQAIFGTGLVKSVED
                     FGLQGEKPSHPELLDWLAVELMDSGWDIKHLLRLIVSSRAYRQSSKTPPGLVERDPEN
                     RLLAHGPRHRLPSWMLRDTALSAAGLLSPAQGGPAVKPYQPAGIWEEATFGKKSYVQD
                     HGESLYRRTLYTFWRRIVGPTMLFDNAARQVCEVKAKRTNTPLHALTTLNDTTYIEAA
                     RALARRVMLAARDDTARVDHLHRLVLARMPSKEERAILLRRLGILKEQFRADPAGAAA
                     LLQVGELPRDERLPAPDHAAFTALAQLVLNLDEALSKE"
     gene            complement(3353..5467)
                     /locus_tag="PLP58_17725"
     CDS             complement(3353..5467)
                     /locus_tag="PLP58_17725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ10424.1"
                     /translation="MQELGAAITSSIPDTPEGRKLVDVTREFFDKDNAIPLALSVLPL
                     GMLGAAASTSRDFAAIEAVMKNPRYLRAVMSEEKAREVMAEPDPNKRAQLIREGWQAT
                     PTAEIARRVQDINAEERAEAAEVLRVIQAAEQAGMIPTIRRDAQGWTVASRNGSAVRV
                     STREEALQMAYSALSDYERQAAEATAQLADEFMDMGEGRAEAFEISRQGATVQSEVEA
                     GRQTPEGAALQVEVFAQIHGLSADEARAERLSVLGSSRMEMIEGVRTAVSRVFGGGNT
                     LTALHEGVHGRWNAEKVEKVPEKVPEKVPRKSARLKVFDRFPSPLHFPTMNPSQSLDS
                     QSDDLEKQLVGVNARTAPYPAARSWSGGRRRILGREVFETNTYHVMSRTCGGEVFFDD
                     VEKEALKRLLWKMSEFCGVRLVTYCVMGNHFHALIEVPKREIWLERFAGAGGEERLFE
                     HLRLLYSKEFIALLRQEIADLRRLGMDALAAAKLERIKRRFCDLSLYVKEVKERFSRW
                     FNKRRGRKGTLWMDRFKSVLVEGKGEPLHTMAAYIDLNPVRAGLVEDPKDYRWCGYGE
                     AMGGSHRARRGLAKVTGKPVDGWGKQGGAEAYRCLLFSTGMEVRDAQNEDVVRKGVTA
                     EEARAVLKEKGKLPAGELIRLRVRYFSEGLALGGKEFVEAVFEANRGRYSAKRKEGAR
                     PIAEVAGGGWYALKRVRNAGAG"
     gene            complement(5870..7639)
                     /locus_tag="PLP58_17730"
     CDS             complement(5870..7639)
                     /locus_tag="PLP58_17730"
                     /inference="COORDINATES: protein motif:HMM:NF017539.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4062 domain-containing protein"
                     /protein_id="HRJ10425.1"
                     /translation="MSTSWKTVRVFISSTFRDMQAERDHLVRFVFPRLPEELLPRRIL
                     LVDVDLRWGVTSDQDALGVCREVIDECRPRFMGMLGGRYGWVPDGKDKSITADEVHYG
                     VLDREPAKRGEAFFYFLEESATAQMVEEEAGEYREPVGSENAGKLAALKESIIAAGLP
                     VFVYPAEWDATQNRLTGLEAFGNQVHDDLLQSLKDDTELASRFTSEATASPDEFAEEA
                     DQMDAFIEERTDRFVLGSREPLMKAMLDFAAADGAPNIFVFTGEPGSGKSAFLAKFTR
                     DVAARHPSSFILPTFIGASTGSTDLRRTLRRLCHELAQSVAGVSDPGTANPASTEPLP
                     FDIKELITHFQKLLTEAAAKQHVVLVFDALNQFDETDGAHWMNWLPRELPPGVRVIAS
                     VIAPVEGEKEHQTLAILRNRQDARVEALKPLDESDTQAIIEGYLKRYSKRLSTEQIAA
                     LKAKPASNLPLYVLTALEELRTLGTYEEITDRIRTLPGDARALFGWILTERLARDPGF
                     RDREGRPCGAALVEKFAACLGVSRHGLSPAELTALLDPGDPLGNVAALLRPPPPPPPP
                     APLPHAPRRTARLLPRPVPRSCQ"
     gene            complement(7854..8231)
                     /locus_tag="PLP58_17735"
     CDS             complement(7854..8231)
                     /locus_tag="PLP58_17735"
                     /inference="COORDINATES: protein motif:HMM:NF016156.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF423 domain-containing protein"
                     /protein_id="HRJ10426.1"
                     /translation="MNPLRLRLAGLLGFSAVLLGALGAHGPVHEKLKAAGGLEHWQTA
                     LEYHLPHALLLAVLALCGGAGGKCAAWAWRSIFAGVLLFSGSLYALALTQIGKLGAVT
                     PLGGLALMLGWLLLALGRWRRDA"
     gene            complement(8303..>8765)
                     /locus_tag="PLP58_17740"
     CDS             complement(8303..>8765)
                     /locus_tag="PLP58_17740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ10427.1"
                     /translation="AGAHAALSGGAESAEVEYAPGFDSGDLAAGLAALRAEEQRQRVT
                     LAGPHRDDLRLTINNLDATTYASEGQQRSMAVALKVAQARALESGFGKAPLLLVDDVF
                     GELDTHRRRALLALLPAGTQKIITTTSLDWADADGLAGRVCRIEGARIAEG"
BASE COUNT         1495 a         2764 c         2868 g         1638 t
ORIGIN      
        1 ccacccccat gttgacaggg tccacggtag gggtgaccgc cgcgacgccg tgagcatcgt
       61 cggcggcgag acctcgcgcg ggccggcgct catcgtcacc gtcagcatga caggcgaggc
      121 gcctgccgga cgctggatcc cgcgcaatgc ggcccgcgcg ggggatcttc ttctcgtcac
      181 gggccgcctc ggaggctcca tccgcgggcg gcatctgcgc ttcgagccgc gcctggccga
      241 ggcgcgctgg ctggcccggc acctgcctgt tcatgccatg atggacttga gcgacggact
      301 ggccaaagac ctgccgcgca tggctgcggc agcgggactc ggcttcgagg ttgagatgca
      361 aagcctgccg cgcgcgcgcg gcgtcaccac ggagcaagcc tggggggacg gcgaggacta
      421 cgagctgctc ctggctgtct cgccgcgcgt caaacccgcc gcccttgcgg cctggcgcgg
      481 cgctttcccc aggctgcccc tgacccgcat cggcactctg acgcggaagc cttccacatc
      541 cttggctccg ggcggatggg atcactttgg ttgaacttca ccccatcctt gcgcgccatc
      601 actctttgct cagtgcctcg tccaggttga gcacgagctg ggcgagagcg gtgaatgcgg
      661 cgtgatcagg ggcggggagt ctttcatcgc gaggcaactc gcccacctgg agcaaagcgg
      721 cggcaccggc gggatcggcg cggaattgct ccttcaagat gccgagacgc cggagcagga
      781 tggcgcgctc ttccttcgaa ggcatgcgcg caagcacgag ccggtgcaga tgatccacgc
      841 gcgcggtgtc atcgcgcgcg gcgagcatga cccgccgggc cagcgcgcgc gcggcctcga
      901 tgtaggtggt gtcattgagc gtggtgaggg cgtgcagggg agtgttggtg cgcttggctt
      961 tgacctcgca gacctggcgg gcggcgttgt caaaaagcat ggtggggccg acgatgcgcc
     1021 gccagaatgt gtaaagcgtg cggcggtaga gtgattcacc gtggtcctgg acgtagcttt
     1081 ttttgccaaa agtggcctcc tcccagatgc ctgcgggctg atagggcttc acagcagggc
     1141 cgccctgcgc gggactgagc aggccggcgg cggaaagcgc cgtgtcccgc agcatccagg
     1201 agggcaggcg gtggcgcgga ccgtgcgcaa gcaggcgatt ctcaggatcg cgctcgacga
     1261 ggccgggcgg ggtctttgaa ctctgccggt aggcgcggct ggagacaatg aggcgcagta
     1321 ggtgcttgat gtcccagccg ctgtccatga gttcgacagc gagccagtcg agcagttccg
     1381 ggtggctggg tttttctccc tggagaccga agtcctccac gctcttgacc agcccggtgc
     1441 caaagatggc ctgccagtag cggttgaccg tgacccgcgc ggtcagcggg ttttcacgcg
     1501 cgacgagcca gcgcgccaga tcaagccggc tcgcgcgatc agggagagcc acgcccccgg
     1561 caaaggcggc gggcacggcg ccggcgactt tgacggcggt gggcttgtca taggcgcctt
     1621 tgtcgaggat gaaagtgtcg cgcggctctt tgagatggtc catgatcatg accttggtga
     1681 cgctgctctg cgcggagtcg cgcgcgcgga cggcggcgag gtgctttttc aatacagcga
     1741 cataggcggg gtccgcgcca tcggtctcga aatagccgac ggcttccaga atggcgtcca
     1801 caccgcgccg ctccgggggt gttttggcga gtgtggcggg caggttgccg gggagcttgg
     1861 aggcgtccga ctgctccagc ggcgcgcctt cggggcgcgg gaattttttc aactcaaagg
     1921 cttcgacctc cttgatgatg gcggccagtt tttgccgggc cgcctccacc cgcccggtct
     1981 cggcgggcgt ggagaaatcg agcacggggg cggcctggcc gccgcgtccc gcgccggttt
     2041 ctgacatctg gttgaagatg tcatagagcg cgtagtagtc gcgctggggc agcgggtcgt
     2101 atttatggtc gtggcagcgg cagcagttga aagtggccgc cagccagaga gtggcggtgg
     2161 tctcgacgcg gtcgaagaca ttctccacgc gcgtctcctc ggcgatgcga ccgccctcgc
     2221 cgttgtgcat gtggttgcgg tggaaaccgg aggcgagctg ctgggagcgg gcggctccag
     2281 gcaggaggtc gccggcgatc tgctcgatgg tgaactggtc gtaaggcatg ttggcgttga
     2341 tggcgtccac cacccagtcg cgccatggcc acatggtgcg ctccgggtcg ccctggtagc
     2401 cgttcgtgtc cgcgtagcgc gcggcatcca gccagtccca ggcccagcgc tcgccaaacc
     2461 gcggactggc gagcaggcgg tccacgatgg agttcaaatc gaaattcgaa gttcgaatct
     2521 cgaagtcgtg cgcgctgggt ggcaggccgg tgaggtcgag ggagaggcgc cgggcaagcg
     2581 tttcggggga ggcatcgggg gagaaggtga gttcttcttt ttgcagtcgc tcggcgatga
     2641 aggcgtcgat ggggttgagt atttcgaatt tcgagcttcg aatttccggc acggccggcc
     2701 gcacaggcgg ttcgtaggcc cagtgtttgc cccagggcgc accctgggcg acccagtcct
     2761 gaatcagttt tttttgttcg gcgctgagtt tgcgcagcgc cttcagaggc ggcatcgcat
     2821 catcggggtc ggtgctgaga atgcgcgcga tgacctcgct tttggacagg tcgccgggca
     2881 cgacggcggt ggcgccgctt tttccccggc ccagcgcgct ttcctgaaga tctagccgca
     2941 gccctccctt ccgccccgct tcatcggggc cgtggcaggc aaaacaagtg tccgaaagca
     3001 cgggcaggat gtcgcgggag aaccgtattt catccgccac ggtgggtgtg gcggcggcga
     3061 gaagcagcgc aaagatggag gctgttgtct gaaaggcgtg ggaatccatg cggtggcact
     3121 caatacgtcg cgtgagggat ggattatccg gccctcgccg tgttctccac cggtcagggc
     3181 caggttcaca aaataccaag tccagaaaat gccttgcaac ttctagtctt tggggcgcac
     3241 ggcatattgg gggcaggggc atgctgcctg tgcgggaagg cgagatgggt ttcccatgcg
     3301 aaaggcaggt ccagagggtg cctgggtcag aacgcacacg gagcagcaca aatcaccccg
     3361 cacccgcatt cctcaccctt ttgagcgcat accatccgcc gcccgccact tccgcgatgg
     3421 ggcgcgcgcc ctccttgcgc ttcgcgctgt agcgtccccg gttcgcttcg aacactgctt
     3481 ccacaaactc cttcccgccc agcgccaatc cctccgaaaa atagcgcact cgcagccgta
     3541 tcagttcgcc cgcaggcagt ttgcctttct ctttgagcac cgcgcgcgcc tcctctgccg
     3601 tcaccccctt gcgcaccaca tcctcattct gcgcatcccg cacctccatg cctgtgctga
     3661 acaacagaca gcgatacgcc tccgctccgc cctgcttgcc ccacccatcc accggctttc
     3721 ccgtcacctt cgccaaaccc ctccgcgccc gatggctgcc gcccatcgct tctccatacc
     3781 cgcaccagcg gtagtctttc gggtcctcca ccagccccgc gcgcacaggg ttgaggtcaa
     3841 tgtaggccgc catggtgtgc agcggctcgc ctttgccctc caccagcacg cttttgaacc
     3901 ggtccatcca cagcgtgcct ttgcgccccc ggcgcttgtt gaaccagcgg ctgaagcgct
     3961 ctttgacctc cttcacatac agcgacagat cgcaaaaccg ccgcttgatt cgctccagct
     4021 tcgccgccgc cagcgcgtcc atgcccagcc gccgcagatc cgcgatctcc tggcgcagca
     4081 gggcgatgaa ttctttgctg tagagcaggc gcaaatgctc aaagagccgc tcctcacccc
     4141 ccgccccggc aaaccgctcc agccaaatct cccgctttgg cacttcaatc agggcatgga
     4201 aatgattgcc catcacacaa taggtgacga gccgcacgcc gcagaactcg ctcattttcc
     4261 aaagcaggcg cttgagggcc tccttctcga catcatcgaa gaagacctcg ccgccacagg
     4321 tgcgagacat gacgtggtag gtgttcgtct cgaagacctc ccgcccgagg atgcgcctgc
     4381 gcccgccgga ccaggaacgc gcggcagggt agggagccgt cctggcattg acgccgacga
     4441 gttgtttttc gaggtcatcg gactgtgaat caagggattg agatggattc atggtgggaa
     4501 agtggagcgg acttgggaag cggtcaaata cttttagcct ggcacttttt cttggcactt
     4561 tttctggcac tttttccggc actttttcca ctttttcggc attccagcgc ccgtgaacgc
     4621 cctcatgaag cgccgtgagg gtgttcccgc cgccgaagac gcgcgagacc gcagtccgaa
     4681 cgccctcgat catttccatt ctgctgctgc ccaggacgga aagcctttcc gcgcgggctt
     4741 cgtcggcgct caggccgtga atttgcgcga agacctcgac ttgcagcgcc gcgccttccg
     4801 gcgtttgccg cccggcctcg acttcgcttt gcaccgtcgc tccctgcctg gaaatctcga
     4861 acgcctccgc gcggccctcg cccatgtcca tgaactcgtc ggcaagttgc gcggttgcct
     4921 cggctgcctg gcgctcgtag tcggagagcg cggaataagc catctgcagc gcttcctcgc
     4981 gcgtgctgac tcgaaccgcg ctgccgttcc ggcttgccac tgtccagccc tgcgcgtcac
     5041 gccggatcgt cgggatcatg cccgcctgtt cggcggcttg gatgacgcgc agcacttccg
     5101 ccgcctccgc tctttcctcg gcgttgatgt cttggacgcg ccgggcaatt tccgctgtcg
     5161 gcgtcgcctg ccagccttcg cggataagtt gcgctcgctt gtttgggtct ggttctgcca
     5221 tcacctcccg cgctttttcc tcgctcatga cagcgcgcag gtatcggggg ttcttcatga
     5281 cggcctcgat ggccgcaaag tcccgcgagg ttgatgccgc cgcgccaagc ataccgaggg
     5341 gcagcactga aagagcaagg gggatggcgt tgtccttgtc gaaaaactcg cgcgtcacat
     5401 ccaccagctt tcggccttcg ggcgtgtctg gaatgctgga agtgatggcc gcgccaagct
     5461 cctgcatgaa tagcgtggtg acttgctgaa agtattcctc tccgaactca atcgcacctc
     5521 gcgcggcaga gcgaccaaga aactgcccaa caaagctgcc tccaggacga gcgccccatt
     5581 ttgccagcaa ttgagcaacg gcgggaatcc ctgccactag ttccgacatc ctttcaatct
     5641 gcccttgaac aatgccggaa gttactgcca ttgcagccgc gtccgctcct gtgtaaccgt
     5701 tctgccgaag gtcgttgtaa gcatcgtcgg agaacatgcc gacaagcgcc aactgccctc
     5761 caggaaccat tgcggcaagc attgttggcg cggaagaagc cccaaaataa aatagtccac
     5821 catcctcaag cgggtggtgc gttgaatgtg atctgtcgtt agatacgcct tattggcagc
     5881 ttcgcggaac tggccgtggt agaagtcgag cagttcgccg cggcgcatga ggtaggggcg
     5941 caggaggcgg aggaggcgga ggagggcgga ggagggcggc gacgttgccc agcggatcgc
     6001 cggggtcgag cagggcggtg agttccgccg gggaaaggcc gtggcggctg acgccgaggc
     6061 aggcggcgaa tttttccacg agcgccgcgc cgcagggccg tccctcgcgg tcgcggaagc
     6121 cggggtcgcg ggcgaggcgt tcggtgagaa tccagccgaa gagcgcgcgg gcgtcgccgg
     6181 gcagggtgcg gatgcggtcg gtgatctcct cgtaggtgcc gagcgtgcgc aactcctcca
     6241 gcgcggtgag gacgtagagc gggaggttgc tggcaggctt cgctttgagc gcggcgatct
     6301 gttcggtgct gaggcgcttg gagtagcgct tcaagtagcc ttcgataatg gcctgcgtgt
     6361 ctgactcatc gagcggcttc aaggcttcga cgcgggcatc ttggcgattc cgcaggatgg
     6421 cgagcgtctg gtgttccttc tcaccctcca ccggagcgat cacggaagcg atgacgcgca
     6481 cgccgggcgg caattcgcgt ggcagccagt tcatccagtg cgcaccgtcg gtttcatcga
     6541 actgattgag ggcatcgaag acgaggacga catgctgctt cgccgcagcc tcggtgagca
     6601 gcttctggaa atgggtgatg agttccttga tgtcgaacgg caggggttcg gtgctggcag
     6661 ggtttgcggt tccggggtca ctgaccccgg ctacagattg cgccagttcg tggcagagac
     6721 ggcgcagcgt gcggcggaga tcggtggagc cggtgctggc accgatgaaa gtaggaagga
     6781 tgaaggatga gggatgacgt gcggccacgt cgcgggtgaa cttggcgagg aaggcggatt
     6841 taccggaacc gggttcaccg gtgaagacga agatgttagg cgcgccatcg gcagcggcga
     6901 aatccagcat cgccttcatc agcggctcgc ggctgccgag gacaaagcga tccgtgcgct
     6961 cttcaatgaa ggcgtccatc tggtctgcct cttcggcgaa ctcatcgggc gacgcggtgg
     7021 cttcgctcgt gaagcgcgag gcgagttctg tgtcgtcctt caggctctgc agcaagtcgt
     7081 catggacttg gttgccgaag gcttcgagtc cagtcagccg attctgcgta gcgtcccact
     7141 ccgccgggta aacaaaaacc ggcagccctg cagcaatgat ggactccttc aatgccgcca
     7201 gcttgcccgc gttctcactg ccgacaggtt cgcggtattc cccagcctct tcctccacca
     7261 tctgcgctgt ggcgctttcc tcaaggaaat agaagaatgc ctccccgcgc ttcgccggct
     7321 cacggtccag caccccatag tgtacttcat ccgccgtgat ggacttgtcc ttgccatccg
     7381 gcacccagcc gtagcgtccg ccgagcatgc ccatgaagcg cgggcggcac tcatcaatga
     7441 cctcgcggca cacgccgagg gcatcttggt cgctcgtcac accccagcgc aggtccacat
     7501 ccaccagaag aatgcgccgt ggcagcagct cctccggcag ccgtggaaac acaaatctca
     7561 cgagatgatc ccgctcggcc tgcatgtcgc gaaacgtgct gctgatgaag acgcggacgg
     7621 ttttccagga ggtgctcatg gcgattgctg agatactaat cagaagcgac tgaaacggcg
     7681 caacggctca agaactgcgg acgtggagcg ctgagggagc gttaggtcag agagttcatg
     7741 gtggatgatg aagcgctgcc gctgcggctt ggtgtggcgt tatttttaga ttaggcgcat
     7801 tgcagcgaag atgagcggcg gaggcgagat tatttttcgc ctgtgtttgg atttcaggca
     7861 tccctgcgcc agcgtcccag cgccagcagc agccagccga gcatcaaagc caggccgcca
     7921 agcggcgtca ccgcgcccag tttgccgatc tgggtgagtg ccagggcata gagcgagcct
     7981 gaaaagagca gcacgcccgc gaagatcgaa cgccaggccc atgcggcgca ttttccgccc
     8041 gcgccgccgc acagcgccag caccgcaagc aggagcgcgt gcgggaggtg atactccagc
     8101 gccgtctgcc agtgctccaa cccgccggcc gccttcaatt tttcatgcac gggaccatgc
     8161 gcgccgaggg cgccaagaag gacggcggaa aatccaagca gaccggcgag acgaagacgg
     8221 agcgggttca tgcggtgacc acaacaagcg cggcctgatg cgcaaatgaa aaatggcccc
     8281 ggctcagccg cgacgatgcg gctcagcctt ccgcgatgcg cgcgccctcg atccggcaga
     8341 ccctgcctgc aaggccgtcg gcgtccgccc agtcgaggct tgtggtggtg atgatctttt
     8401 gcgttcctgc cggcagcagg gccagcagcg cgcggcggcg gtgcgtgtca agttcgccga
     8461 agacatcgtc caccagcagc agcggcgcct tgccaaagcc ggactccagc gcgcgcgcct
     8521 gcgccacttt caatgccacg gccatgctgc gctgctgccc ttcggaggcg taggtggtgg
     8581 cgtcgaggtt gttgatggtt aggcgaagat cgtcgcggtg cgggccggcc agggtgacac
     8641 gctggcgctg ctcctccgcg cgcaaggcgg cgagtcctgc ggccaggtcc ccgctgtcaa
     8701 aaccgggcgc gtactccacc tccgccgatt ccgcgccgcc gctgagcgcg gcgtgcgcgc
     8761 cagcc
//
LOCUS       DAOMYJ010000214         8743 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Prosthecobacter sp. isolate AMR_MDS_5629
            NODE_2674_length_8743_cov_22.537293, whole genome shotgun sequence.
ACCESSION   DAOMYJ010000214 DAOMYJ010000000
VERSION     DAOMYJ010000214.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867850
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prosthecobacter sp. (wastewater metagenome)
  ORGANISM  Prosthecobacter sp.
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Verrucomicrobiaceae; Prosthecobacter.
REFERENCE   1  (bases 1 to 8743)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8743)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.30
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:34:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,742
            CDSs (total)                      :: 4,684
            Genes (coding)                    :: 4,676
            CDSs (with protein)               :: 4,676
            Genes (RNA)                       :: 58
            tRNAs                             :: 55
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8743
                     /organism="Prosthecobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2674_length_8743_cov_22.537293"
                     /isolate="AMR_MDS_5629"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1965333"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..322)
                     /locus_tag="PLP58_17745"
     CDS             complement(<1..322)
                     /locus_tag="PLP58_17745"
                     /inference="COORDINATES: protein motif:HMM:NF013855.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="repressor LexA"
                     /protein_id="HRJ10428.1"
                     /translation="MPRSLRKITERQKQMLEYLRSYQREHGVMPSTRDIQRHFEFASQ
                     TAAMSHLRALERKGLIQRHPHMARAVVFPADLKRPEIVDVPLYGSIAAGYAENQEQQA
                     DGSIS"
     gene            complement(517..5358)
                     /locus_tag="PLP58_17750"
     CDS             complement(517..5358)
                     /locus_tag="PLP58_17750"
                     /inference="COORDINATES: protein motif:HMM:NF037015.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HRJ10429.1"
                     /translation="MNTPMRSLQAGAFCLFHTVLLAGPSAPSAMPHDPWRAAAQAACQ
                     GDGISLKPATEGAELRVVFQKLAGRVTGEGLWLESTEKAGRAERFRVRAAALGGATPA
                     ELAAHGGVSVEGGTARYTRPGLVEEFRVSLDGVRQDFVVETPPVDGGGLNVWLEISGA
                     KAAPAAYGARLTLDGSGRALAYSRLHVTDARGRVLPARMEVAAADRLVIRVDDAGAAY
                     PVRIDPTFSDEDWISLGGQAGVRGSVHAVALDNNGNLYVGGQFDAVGELEANNIARWN
                     GTAWSTLGKGVNGEVLALAFAVEQSTLFVAGEFTEAGGAPAASVAAWNVDTQDWSALG
                     TGISGGPVTALHFEFGQGLFAGGHFDEAGGTAGTARVALWNGSWSGFDDGPGGIGDEL
                     SSVTYFQGKVVAGGEFGILEWDGLAWVPVGGGVGGSLPYVHALAVGDFGNVMYAGGEF
                     TTAGGIPAARIASWDGVEWRALGSGMNDWVGELRAAGSNLYAGGLFTTAGGTAAHKLA
                     FWNGSAWSAVLGGEQINGSVDAIAVWGGDVYLGGAFTKATTVEADGLMRWNGSALSSI
                     AGKGLNGSVHASLVHEGELYLGGSFTNAGGMIVNRIVKWNGNAWSALGAGVGGRVNAL
                     AAIGGGIYAGGLFTQAGGSSANRVARWDGTAWHALGAGVNGEVLALASMGTDLFAGGL
                     FSSAGADTNIKRLAKWDGSSWSSVGGVAFEGTVRCLAVTGGDLYAGGSYSQNVAGVDL
                     RRIAKWDGTAWSELGGGLNSDAYALAFSGSDLIVGGSFTSFGPATAPIAAARIARWDG
                     SAWSTFGSGITGTGAIVRALAAHDSGIYAGGSFTTAGGSAIPHLARWTGGAWEGLASG
                     VNAQVHTLTVAGDVLHAGGEFDLAGGKISHFAAQANLVPDAGTSVISFSQDVNETTQG
                     ANEVTITLQRTNATQAASVRLSTANGTNSSNPPFTGGRAGTDYAALSNTLVEFDAGQS
                     SKNVTLTLIPRTGAVPNIRFRAILGSPSANADIGLGGAEIRILANDATAPTVNITYPT
                     EGFAFSNVPPINIVGTAGDSLGIREVTLSFNGGPPVQASLGTSSNPLNTPFSGSFTPL
                     EGANTIVATAYDLRGNSSSVTRNFTFTRSYTLTVTRNVPPAASANPDLAGTILLKASP
                     TTGATRLLPAARTSPQTANVTPVTEVKLTARPKTGYFFSHWSGLPADFQLAGTGVTFI
                     MPAEDAPNVTATFVTSPFTGLPDGSRINFYGQVRPDAGTPVNNSTVGFLNATLTTSTG
                     ALSGKLLMDGESRSFSARILGDGSVWYNVGGFVGTPISFGPKILYLEMVSGVLLIEVV
                     NTAIGGSSKGAAKPAIYSAANPVPAALINEPAAIPQPNGYYTLALPPVVQAPPMDTST
                     YPKGAGYATLNIAGHGGLKLSGVLADGTKFTAASALVEGDAAPFFGQLPTPGDTSVKG
                     GSIGGDLIFDASQPDSDILGADMLWLRPAAAVPLYPAGWPAGITVDVIGALYDKTAGA
                     QTLLGMGPVDPANGNGRLTFTEGTLPAPVVKTTFNVSGNLVVKVPPVPVDRSFNLTIS
                     NPLHGSFKGDFVPSGSTLKLKYSGVIVSKGALRAGYGYFLSSPISASPPQSGHALLEA
                     RTP"
     gene            complement(5377..>8743)
                     /locus_tag="PLP58_17755"
     CDS             complement(5377..>8743)
                     /locus_tag="PLP58_17755"
                     /inference="COORDINATES: protein motif:HMM:NF039835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ10430.1"
                     /translation="TVRELTNNAEIAGRTPLAAPVPGADGRLWGVTSLGGEKNQGTVY
                     AFDAVNDVFETEHEFTYDGLVPEQLNKGSKPHGGLVSDGAGFLWGATEFGGPHDRGTI
                     YKIHETTSEVTVTHVFSNAAGSPNYPRSPLVKAGGFLWGMTATGGTGNHGTIFKVDIS
                     NGSVTKVVDFTNTSAPAKGRLPYGALLDDGTGVLWGTTSQGGNGNGTVFTFDTSTEEF
                     TTVVEFTDNGLTNKGAEPFAGLVDDGEGYLWGTTSRGGQGGSSSRIGTVFKILKSSGE
                     LTTVLFFTGGADTANPGRAPRSGLVNDGEGSLWGTTYEGGEFNLGTIYRVKINTGELT
                     SVLSFKGNGTGAGAGSSPSYGGLYLHSDGSFYGTTETGGSGGAGTLFRLEVETTPPEF
                     SLEQEVYTVNQGGTEVLLTVERTGGSAPASVTLGLANGMASLVPPFAAAVADTDYLPL
                     TLQQSTLDFAEGEMAKQVTVTLLPKTGKNLPNKRFTATLSDPTNGAVLGPVPQATVNI
                     LADDTTKPTVKLVTPAAKVNAAVPYLLTGVAGDARGIAKVEVVLNGGPVIEAALGASV
                     KPAAVPFSLPLALPVSGGTLIEGVNMVVVTAYDLRGNTASVTRKFTFIRRLGRLIVNR
                     FDHGVYFGNDPEPPQSLGVVTFKAANATAFAPYWVTPGSYYSDFVPGSKGVLTAKPNK
                     GFVFARWFGSPGDSVTDLGPILEFTTREGASTLQAEFIRNPFLPPTAGMSNIFNGVIT
                     PVNATPVENDSHGRLTGTLVPATGAFSGQIQVAGVTQKFAAFFTGLGQCYFVAGKTRP
                     RQITVGGWTLTLYLDVDEQAIQVALTKPGGLRCEGLARRALYDGKISLPPQNLRNLGA
                     KGFFTFATSADGPMEMSRDEMPNGFGIGTLTLDLKGNVILAGTLADGTPVTCSSALVA
                     GNTLPVFCPLQTPGAKKGVNGGCFGGEVVLAPTEFDGDLQGEDLFWFRPAVTQLTGVT
                     PAALATQLYTSGWPGGLSGRIFGALYDPKLTLQAMLDLLEAGAEPILRVQEGRLTDMV
                     DVTSFSIQGNKIVKDNPADKTFDIKTTLAKGAFNGVFTPNWPSPSSKLPAFRGILIQG
                     GTMVSGGYGFSISNAAGDLDPESSAVFFNRLND"
BASE COUNT         1512 a         2851 c         2805 g         1575 t
ORIGIN      
        1 cggagatgga gccgtccgcc tgctgctcct ggttttcggc ataaccggcg gcgatgctgc
       61 cgtagagcgg cacatccacg atttccgggc gtttcaaatc cgcagggaaa accaccgcgc
      121 gcgccatgtg cgggtggcgc tggatgaggc ccttgcgctc cagggcgcgc aggtggctca
      181 tggccgccgt ctggctggca aactcgaagt ggcgctggat gtcgcgcgtg ctgggcatca
      241 cgccgtgctc gcgctggtag ctgcgcaggt attccagcat ctgtttctgg cgttcggtga
      301 ttttgcggag ggatcgtggc atggcagagg attctgttct gccgaacatt tggtattatg
      361 gacatcatgc aagcacttta ttgccctttg gcaccgacgg gttgggaatg gtgatttcat
      421 ggcgtgacca aggcatcctc cgtcaactcc gccgcggcgg aggccgcctg ctgagcgcca
      481 tccaagggcc tcatcgggaa tcaaagtgct gtcagttcat ggcgtccgcg cctccagcag
      541 tgcatggccg ctctggggcg gcgaagcgct gatgggactg ctgaggaaat agccataccc
      601 tgcccggagc gcgcctttgg agacgatgac tccggaatac ttcagcttga gagtgctgcc
      661 gcttggaacg aaatcgccct tgaagctgcc gtggagcgga tttgaaatgg tgagattaaa
      721 gctccggtcc acaggcacgg gcggcacctt gacgaccagg ttgccgctga cattgaaggt
      781 ggtcttgacc acgggagcgg gcagcgtgcc ttccgtgaag gtgagtctgc cgttgccatt
      841 ggccgggtcc acgggtccca tgccgaggag cgtctgcgcg cccgctgttt tgtcgtagag
      901 agcgccaatg acatccaccg tgattccggc aggccagccc gctgggtaga ggggcactgc
      961 ggctgccggg cgcagccaga gcatgtccgc gccgaggatg tccgaatcag gctggctggc
     1021 gtcaaaaatc agatcaccgc cgatgctgcc gcctttaacg gatgtgtcgc cgggcgtggg
     1081 tagttggccg aagaagggtg ccgcatcgcc ttcgacgagg gcggaggcgg cggtgaactt
     1141 ggttccatcc gccaggacac cgctgagctt cagaccgccg tgcccggcga tgttgagggt
     1201 ggcgtagccc gcgcccttcg gataggtgct ggtgtccatg ggcggagcct gcaccaccgg
     1261 gggcagggcc agggtgtagt aaccgttggg ctgcggaata gccgccggtt cgttgatcaa
     1321 cgcggcgggc acagggttcg cggcgctata gatggcgggc ttggctgcgc ccttgctgct
     1381 gccgccaatg gcggtgttga ccacctcgat cagcaacact cccgagacca tctccaaata
     1441 gagtatcttg ggaccgaagc tgattggtgt gccgacaaat cctccgacat tataccaaac
     1501 cgatccgtca cccagaatgc gggcggagaa ggatctggat tcaccatcca tgagcagctt
     1561 gccgctcaag gcgccggtgg aggtggtgag tgtggcgttc aaaaagccga cggtgctgtt
     1621 gttgacaggg gtgccagcat caggacggac ctggccgtaa aagttgattc gtgatccatc
     1681 cggcagaccg gtgaaagggc ttgtcacaaa tgtggcggtc acattcggtg cgtcttccgc
     1741 tggcatgatg aaggtgacgc ctgtgcccgc caattggaaa tcggctggca gaccgctcca
     1801 atggctgaaa aagtaacctg ttttcgggcg ggctgtcagt ttgacttcgg tgacgggagt
     1861 cacatttgcc gtttgcggac tggttctcgc agctggcaaa agacgagtcg cgccagtggt
     1921 tggcgaggct ttgaggagga tggttccggc gaggtcggga ttcgcgcttg ctgctggcgg
     1981 cacgttccgg gtgaccgtga gcgtgtagga gcgagtgaag gtgaaattcc gcgtcacgct
     2041 gctgctgttg ccgcgcagat cataggcggt ggccacgatg gtgttcgcgc cttccagcgg
     2101 cgtgaaggag ccgctgaagg gcgtgttcag aggattggac gaggtgccca ggctggcctg
     2161 gacgggcgga ccgccattga aggagagtgt gacctcgcgg atgccgaggg aatcacccgc
     2221 cgtgccgacg atgttgatcg gaggaacgtt ggaaaaagca aagccttccg tggggtaggt
     2281 gatgttcacc gttggagcgg tcgcgtcatt ggcgaggatg cgaatctccg cgccgccaag
     2341 tccgatgtcc gcgttggcgg acgggctgcc caaaatcgcg cgaaagcgga tgttcggcac
     2401 cgcccccgtg cgcgggatga gggtgagggt cacgtttttg ctgctctgac cggcgtcgaa
     2461 ctccaccagc gtgttgctca gcgcggcata gtctgtgccg gcgcgtccgc cggtgaaggg
     2521 gggattgctg ctgttggtgc cattggcggt gctgaggcgg acactggctg cctgtgtggc
     2581 attggtgcgc tgaagggtga tggtcacttc attcgctccc tgggtcgtct cattgacatc
     2641 ctggctgaag ctgatcacgg aagtccccgc atcaggcacc agattcgcct gtgcggcgaa
     2701 gtgtgagatt ttgccgcccg ccaggtcgaa ctctccgccg gcgtggagca catctccggc
     2761 aacggtcaga gtatgcacct gggcattgac tcccgatgcc agcccctccc aggcgcctcc
     2821 agtccagcgg gccaggtggg ggatggccga gccgcccgcc gtggtgaagc tgcctccggc
     2881 gtagatgccg ctgtcatgcg cggccagcgc ccgcacgatc gccccggtgc cggtaatgcc
     2941 cgaaccgaag gttgaccatg cactgccatc ccacctggca atgcgcgcgg cggcaatcgg
     3001 agccgtggcc ggcccgaagg acgtgaacga cccgccaacg atcagatccg agcccgaaaa
     3061 ggccagggcg taggcatcgc tattcaatcc cccgcccaat tcggaccatg cggtgccatc
     3121 ccatttggcg atgcggcgca agtccacgcc agcgacgttt tgactgtacg agccgcccgc
     3181 gtacaagtcg ccacccgtga cggcaaggca gcgcaccgtc ccttcaaaag ccaccccgcc
     3241 caccgagctc caggatgagc catcccactt ggccaggcgc ttgatattcg tgtccgcccc
     3301 ggcgctggaa aaaagcccgc ccgcaaacaa atccgtgccc atggatgcca gtgccaaaac
     3361 ctcgccattc actcccgcac ccagcgcgtg ccaggcggtg ccatcccagc gcgccaccct
     3421 gttcgcggat gaaccgccgg cctgtgtgaa gaggccgccg gcataaatgc cgccgccgat
     3481 ggcagccagc gcgttgaccc gtcccccaac tccggcgccc agcgcgctcc aggcgttgcc
     3541 gttccatttg acgatgcggt tcacaatcat cccgcccgca ttggtgaaag agccgcccag
     3601 ataaagctcg ccttcatgca ccagggaggc atgaacactg ccgttcagcc ctttgcccgc
     3661 gatgctggat agcgcgctgc cattccagcg catcaggccg tcagcctcca ctgtggtggc
     3721 ctttgtgaaa gcgccgccga gatagacatc gccgccccag accgcgatgg catccactga
     3781 accgttgatt tgctccccgc caagcacggc ggaccatgcg ctgccattcc agaaggccag
     3841 cttgtgcgcc gcagttccgc ctgccgtggt gaacaggccg cccgcgtaaa gattcgaacc
     3901 tgcggcacgc agttcgccga cccagtcgtt catgccggaa cccagcgccc tccactcaac
     3961 gccatcccag ctcgcgatgc gcgcggcggg aatgccgccc gccgtggtga actcgccccc
     4021 cgcatacatt acgttgccga agtcaccgac cgccagcgca tgcacataag gtagtgagcc
     4081 gcccacacct ccgcccacag gcacccaggc caggccgtcc cactccaaaa tgccaaactc
     4141 cccgcctgcc acgactttgc cttggaagta ggtcaccgag ctgagttcgt ccccaatgcc
     4201 gcccgggccg tcatcaaacc ctgaccagga accattccat agcgccactc tggcggtgcc
     4261 ggccgtgccg cccgcctcgt cgaagtggcc tcccgcgaac aagccctgac caaactcaaa
     4321 gtggagagcc gtcacaggtc cgccgcttat gcccgtgccg agtgccgacc aatcctgcgt
     4381 gtcaacattc cacgccgcca ccgacgcggc cggtgctccg cccgcctcgg tgaactcgcc
     4441 tgccacaaaa agcgtgcttt gctccacggc aaacgcgaga gccaggacct cgccattcac
     4501 acccttgccc agggtgctcc aggcggtgcc gttccagcgg gcgatgttgt tggcctccaa
     4561 ttccccgaca gcatcaaact gaccgcccac atagaggttg ccattgttgt ccaaagccac
     4621 ggcatggacg ctgccgcgca cgcccgcttg cccccccagg ctgatccagt cctcgtcact
     4681 gaaggtcggg tcaatgcgca ccgggtaggc ggcgcccgcg tcgtccacgc ggatgaccag
     4741 ccggtccgcc gccgccacct ccatgcgcgc gggcagcacg cgtccgcgcg cgtccgtcac
     4801 atgcaggcgg ctgtaggcca gggcgcgtcc gctgccatcc agggtgagcc gcgcgccata
     4861 ggcggcgggg gcggctttgg cgccggaaat ctccagccac acattcagcc cgcctccatc
     4921 cacgggcggc gtctccacca caaagtcctg ccgcacgccg tccaggctca cgcggaactc
     4981 ctccaccaag ccgggacgtg tgtagcgagc ggtcccgcct tccacgctga caccgccatg
     5041 cgccgccagc tccgcaggcg tcgcgccacc cagggctgcc gcgcgcacgc ggaagcgctc
     5101 cgccctgcct gccttctccg tggactccag ccacaagcct tcccccgtga cgcgtcccgc
     5161 caatttttgg aagacgacac gcagttcggc tccctccgtc gcaggcttca gggagattcc
     5221 gtcaccttgg caagccgcct gggcggcggc cctccaagga tcatgcggca tggcggaagg
     5281 tgcggacggg ccggccagaa ggacggtatg aaacaagcag aaggctccag cctgcaagga
     5341 gcgcatgggg gtgttcatgg gctttggtgc cagggtttag tcattgagac ggttgaaaaa
     5401 cacagccgaa gactcgggat ccaaatctcc ggcagcattg ctgatcgaga aaccgtaacc
     5461 gccggagacc atggtgccgc cttgaatcag gatgccgcgg aaggcgggca gcttgctgga
     5521 gggtgagggc cagttgggcg tgaagacccc gttgaaggcc cccttcgcca gcgtggtctt
     5581 gatgtcgaag gttttgtccg caggattgtc tttgacgatc ttgttcccct ggatgctgaa
     5641 gctggtgaca tccaccatgt ccgtgagcct gccctcctgc acgcgcagaa tcggctcagc
     5701 gcccgcttcc agcagatcga gcatggcctg gagggtgagc ttgggatcgt agagcgcccc
     5761 gaagatccgc ccgctcagac cgcccggcca ccctgacgtg tagagctgcg tggccagcgc
     5821 cgccggtgtg acacctgtca actgagtgac cgccgggcgg aaccagaaaa ggtcctcccc
     5881 ttggagatcg ccatcgaatt ccgtgggcgc cagcaccact tcaccgccga agcagccgcc
     5941 gttgacgcct ttcttggctc cgggggtctg caaggggcag aagaccggca gcgtgtttcc
     6001 ggcgaccagg gcggaggagc aggtgacagg tgtgccgtcc gccagggtgc cggccaggat
     6061 cacgttgcct ttgagatcga gcgtgagagt gccgatgccg aagccgttgg gcatctcatc
     6121 ccggctcatt tccatggggc catccgcgga ggtggcgaag gtgaaaaagc ccttcgcgcc
     6181 cagattgcgc aggttttgcg ggggcaggga gattttgcca tcgtagagcg cgcgccgcgc
     6241 cagcccctcg cagcggagtc cgcccggctt ggtgagagcc acctggatgg cctgctcgtc
     6301 cacatccaga tacagggtca gcgtccagcc gccgacggtg atctgcctgg gccgcgtctt
     6361 gcccgccacg aaatagcact ggccgaggcc ggtgaaaaag gcggcgaact tctgtgtcac
     6421 gcccgccacc tggatctgcc cgctgaacgc gcccgtggcc ggcaccaggg tgccagtgag
     6481 ccggccgtgg ctgtcatttt ccacaggcgt ggcgttcacg ggcgtgatga cgccgttgaa
     6541 aatgttgctc atgcccgctg tcggcgggag gaagggattg cggatgaact cggcctggag
     6601 cgtgctcgcc ccttcccggg tggtgaactc caggatgggg ccgagatccg tcaccgagtc
     6661 cccaggcgag ccaaaccacc tggcaaagac gaagcctttg ttcggcttgg cggtcagcac
     6721 ccccttcgag ccgggcacga agtcggagta atacgagcca ggcgtcaccc agtagggggc
     6781 gaaggcggtg gcattggcgg ctttgaacgt caccacgccc aggctttggg gcggctcggg
     6841 gtcgttgcca aagtaaacgc catgatcgaa gcggttgacg atcaggcggc ccaatcgccg
     6901 gatgaaggtg aacttgcgcg tgacactggc ggtgttgccg cgcaggtcgt aagccgtcac
     6961 caccaccatg ttgaccccct cgatcaaagt gccgccgctc acaggcagcg ccagaggcag
     7021 ggaaaacggc accgccgcgg gtttgaccga cgcgcccagc gctgcctcaa tgaccggacc
     7081 gccattgagc acgacctcga ctttggcgat gccgcgcgcg tcacccgcca cgccggtgag
     7141 caggtagggc accgccgcgt tcaccttggc ggcgggggtg acgagtttga ccgtcggttt
     7201 ggtggtgtca tcggccagga tgttcaccgt ggcttgcggc acgggtccca gcaccgcgcc
     7261 attggtggga tcggagagcg tggccgtgaa gcgtttgttc ggcaggtttt tgcccgtttt
     7321 tggcagcagc gtcactgtca cttgcttcgc catttcgccc tcggcgaagt cgagcgtgga
     7381 ctgctgcagg gttagcggca ggtaatccgt gtccgccacc gccgcggcaa aaggcggcac
     7441 caggctggcc atgccattcg ccagacccag cgtgaccgag gcgggggccg agccgccggt
     7501 gcgctccacg gtgagcagca cttccgtgcc gccctggttc acggtgtaaa cctcctgctc
     7561 cagggagaac tccggcggag tcgtctccac ctccagccgg aagagcgtgc ccgcgccgcc
     7621 gctgccgccc gtctccgtgg tgccgtaaaa gctgccgtcg ctgtgcaaat acagcccgcc
     7681 gtagctggga gaagaccccg cgcctgctcc cgtgccattg cctttgaagc tcagcacgct
     7741 ggtcagctca ccggtgttga tcttcacccg gtagatggtg cccagattga actcgccgcc
     7801 ttcataagtg gtgccccaaa ggcttccctc cccgtcattc accagccctg agcgcggcgc
     7861 gcgtcccgga ttggccgtgt ccgcgccgcc ggtgaagaac agcaccgtgg tgagctcgcc
     7921 gctgcttttc aggattttaa agaccgtgcc gatgcggctg ctggagccgc cctggccgcc
     7981 acggctggtg gtgccccaga ggtatccctc cccgtcatcc accagccctg cgaagggctc
     8041 cgcccccttg ttggtcaggc cattgtcggt gaactcgacg accgtcgtga actcctccgt
     8101 gctcgtgtcg aacgtgaaca ccgtgccgtt gccgttgccg ccctggctgg tcgtgcccca
     8161 gagcacgccc gtgccgtcat ccagcagtgc tccatagggg aggcggccct tggcgggggc
     8221 gctggtgttg gtgaagtcca ccaccttggt cacggacccg ttggagatgt ccactttgaa
     8281 gatcgtgcca tgattgccag tgccgcccgt ggcggtcatt ccccacagaa agccgcccgc
     8341 cttcaccagg ggggaccttg ggtaattcgg gctgccggcg gcattgctga aaacatgcgt
     8401 cacggtcacc tccgaggtgg tctcatggat tttgtagatc gtcccgcgat cgtgcgggcc
     8461 gccgaactcc gtcgcccccc agaggaagcc cgcgccatca ctcaccaggc cgccatgcgg
     8521 tttgctcccc ttgttgagct gctcgggcac caggccgtcg tatgtgaact cgtgctccgt
     8581 ctcgaagacg tcattcacgg catcgaaggc atagaccgtt ccctggttct tttcgccgcc
     8641 caggctcgtc accccccaga gccggccatc cgcaccgggg actggcgcgg ccagcggtgt
     8701 ccggcccgcg atctccgcat tgttcgtcag ctcccgcacc gtc
//
LOCUS       DAOMYJ010000215         8693 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Prosthecobacter sp. isolate AMR_MDS_5629
            NODE_2692_length_8693_cov_24.052211, whole genome shotgun sequence.
ACCESSION   DAOMYJ010000215 DAOMYJ010000000
VERSION     DAOMYJ010000215.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867850
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prosthecobacter sp. (wastewater metagenome)
  ORGANISM  Prosthecobacter sp.
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Verrucomicrobiaceae; Prosthecobacter.
REFERENCE   1  (bases 1 to 8693)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8693)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.30
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:34:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,742
            CDSs (total)                      :: 4,684
            Genes (coding)                    :: 4,676
            CDSs (with protein)               :: 4,676
            Genes (RNA)                       :: 58
            tRNAs                             :: 55
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8693
                     /organism="Prosthecobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2692_length_8693_cov_24.052211"
                     /isolate="AMR_MDS_5629"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1965333"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..231
                     /locus_tag="PLP58_17760"
     CDS             <1..231
                     /locus_tag="PLP58_17760"
                     /inference="COORDINATES: protein motif:HMM:NF037012.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HRJ10431.1"
                     /translation="ATIHQDADIYRVRLPKGQSITHEVKTGRGVWFQLIKGSITTHGS
                     TLTPGDAFSTEDTGTLTLTASDDAEALLFDLA"
     gene            268..657
                     /locus_tag="PLP58_17765"
     CDS             268..657
                     /locus_tag="PLP58_17765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ10432.1"
                     /translation="MKHASTLASVLLGLAFITFGANFFLNFIPMPADPSPADAPHKLF
                     MAAMFPTGYLAFVKVLEILGGLLVALPKTRNIGLLVLGPIIVNILAFHVFLTKGAGLT
                     DPPILVISILSAFLLFTERKAWLGLVK"
     gene            complement(663..1070)
                     /locus_tag="PLP58_17770"
     CDS             complement(663..1070)
                     /locus_tag="PLP58_17770"
                     /inference="COORDINATES: protein motif:HMM:NF023276.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3368 domain-containing protein"
                     /protein_id="HRJ10433.1"
                     /translation="MLQSLYGRIYVPVAVWHELVVDGRGRAGAETVGGCDWIESRSAT
                     DREMVAILETTLDPGESEAIALAKEMQADFLLLDEALGRAAAHHIGLKTTGLLGVLLE
                     AKRRGVLPDAYELALAMKAEGWWVSEALLERLR"
     gene            complement(1126..1386)
                     /locus_tag="PLP58_17775"
     CDS             complement(1126..1386)
                     /locus_tag="PLP58_17775"
                     /inference="COORDINATES: protein motif:HMM:NF015630.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0175 family protein"
                     /protein_id="HRJ10434.1"
                     /translation="MSGMISLQLPADVLNAAGMTLDELRLEMAIHLFEQRRLSLGRAA
                     RFAGMEKGDFVPLLGQRQISIFGDEEQMVKDAEMLSQLRLPA"
     gene            1416..2324
                     /locus_tag="PLP58_17780"
     CDS             1416..2324
                     /locus_tag="PLP58_17780"
                     /inference="COORDINATES: protein motif:HMM:NF016176.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HRJ10435.1"
                     /translation="MSTPMTAHELQTLLSSADAPLLVQVLPEEVFAARRIPGSKQACV
                     YEVAFFDQIAALTSDKARAIIVYGAGSGSLDAKVAAEKLVAAGFTNVSAYDGGLDAWS
                     AAGLPLEGEGTLPAAPSLSGTFRADTEQSLIRWTGRNLFNHHSGIVRLADGEITLRDN
                     ALVAARFRIAMDSIACEDISDSAMNQMLIAHLHSDDFFDVAHHPVAEFVAESITPLAT
                     CTDGTPNYLLRGQFTLRGITREIEFPILAATADGKRLTAQGVLDLDRTQFGSVYGSGR
                     LFRFLGKHIVNDHVHLHIKLHADLQS"
     gene            complement(2350..3219)
                     /locus_tag="PLP58_17785"
     CDS             complement(2350..3219)
                     /locus_tag="PLP58_17785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ10436.1"
                     /translation="MEEVAAHDMQVSDAARAALLRDVGGDGDGIAVEHAVVAVERRDA
                     QTDTIRAPDADGGIEHFEHQTGAIFDGAAVRAAALVGAIAEEFVEQVAIGSVDLDAVK
                     TGRFGVLGGFFESGDDAGDLVVAEFTRCDVGFLAFGRVNFIAGDRNGARSHRLGSAIE
                     QRMTGTTAMPELEDDLAAFLMHGIGDGFPAFDLSIGVDAGLHPEGGIALHDHRGLGDD
                     QTGAGPLRVVFGHERRGIVIGIGTATRERGHEDTVGELQSAGGERGEEVHGVVEFWSD
                     VLGKGETGTLACF"
     gene            3233..3613
                     /locus_tag="PLP58_17790"
     CDS             3233..3613
                     /locus_tag="PLP58_17790"
                     /inference="COORDINATES: protein motif:HMM:NF012927.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ10437.1"
                     /translation="MSSRRRDSDPVAITTYIAQQCSTRGIAYLHVMRSDFFQAQQGDV
                     LTPARQHFKGTLISNMGYTADEAEQAITEGKLDAVAFGTSFLANPDLPARIAAKAPLN
                     APNPAKFYSPGPEGYTDYPFLLAV"
     gene            complement(3718..4359)
                     /locus_tag="PLP58_17795"
     CDS             complement(3718..4359)
                     /locus_tag="PLP58_17795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009959937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HRJ10438.1"
                     /translation="MSQLLDYILHVDKYLQAFAQEYGTLIYILLFAIVFCETGLVVTP
                     FLPGDSLLFAVGALAAQDLIRLEIILPLLITAAIIGDNLNYWIGRKAGGWMVRQRWFK
                     NDYLVKTEAFFVRHGGKAIILARFAPIIRTFAPFTAGLGRMHYTRFLTFSLAGGVIWV
                     VSFGLGGYFLGSIPVIKNNLKLVFLLIIVVSLLPIIIEVVRHKRDAKKAAAAE"
     gene            complement(4414..5685)
                     /locus_tag="PLP58_17800"
     CDS             complement(4414..5685)
                     /locus_tag="PLP58_17800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009959221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HRJ10439.1"
                     /translation="MTTSPKWYSGVTRYQWLVLTVASLGWVFDAFEGQLYNITRGDML
                     PDILKVGADDPLVKAWGERFLGIFLIGGTIGGWLFSSMADRIGRNPVLALTILFYSIF
                     SGLTYFATEAWHVGVLRFLVAMGVGGEWAVGAALVAEVFPKQARERAGGIFHATSVAG
                     LWLAAAAGLWVGTEWRTAYLLGVIPALLVLWVRLSIKEPEAWKQAREQKSDRLGSFAE
                     LLGDPRWRSRAVFGALLAMVGLATFWGVVVAGQNIAEHLLKQLGDPEWASKSKIAFGF
                     IQTAGAGAGMLAFGPLSARWGCKKTFVVMHLAALVMTPVVCWVPSTFGNYALLLVLLP
                     VFGFFAQGIHAGYAAYFPALFPTHLRATGSGFCFNTGRILAAPVLIWLSAWMKAAFDL
                     RVAVTCLGGFFLLGLVFLALLPETRGEDLPE"
     gene            5775..6482
                     /locus_tag="PLP58_17805"
     CDS             5775..6482
                     /locus_tag="PLP58_17805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009964698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ10440.1"
                     /translation="MPASSFPPNHPRIRVVGAFDELVTARFESGVNALCWPRVLEGDF
                     EEVVMKLKAGPGITHLTEDMLRALPLSAAGRLAAAAMLDDYQRLADHGLQPALDCVNG
                     YEHAEETGPVRTDVCSWHADSATAEVDTWLCTYHGASTEGLINEEAIRRVDVPETRAE
                     LLKLYGGADDGDFREWLNERFYDLHYAPLPHARPYAFGAGNLWRIATLHDSCAVPPCV
                     HRAPGFVPGQKRLLLIS"
     gene            complement(6541..7821)
                     /locus_tag="PLP58_17810"
     CDS             complement(6541..7821)
                     /locus_tag="PLP58_17810"
                     /inference="COORDINATES: protein motif:HMM:NF013567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HRJ10441.1"
                     /translation="MSTRRSFLATTSGLALSAATHAFASGGSSDIKIALIGCGGRGTG
                     ACNQALSAGSTIKLVAVVDPLEGKAQAALDILKQKHEGQVDVPPDQVFTNFEDYQKAF
                     ALADVVLITTPPGPRPFLFEQAVKAGKHVFMEKPVAVDAMGARRVIAAAQEAKKKKLN
                     VCVGFQRRYDPAYMYVINRIHAGEIGDLVYGRVYWNGSSRPGFPREPGESELHYQIRN
                     WYFFTWMSGDHILEQHCHNLDVANWVLQGNMPVRATGQGGRQVRRAKENGTIFDHHTV
                     EFEYEGGFRLLSQCCQIGGKCQRDVSEHFHGLKGTANLAANGRFLINGQPPGGKRARN
                     KTDAYQIEHDAFFENIRTGGYRNDAEYAAHSTLMAVMGRMATYTGQVITWDQVMNSRE
                     NLAPDNLTWDSEPPVKPDADGWYPVAVPGATLPL"
     gene            complement(7854..>8693)
                     /locus_tag="PLP58_17815"
     CDS             complement(7854..>8693)
                     /locus_tag="PLP58_17815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ10442.1"
                     /translation="PMSQEPTPWSLKKIWALEAGAGEGVLWCGTIPGGLFHSTDHGAS
                     WRLVESLWNHPDRAKWFGGGADWPGIHSILIDPRDKQRLLIAVSCAGVWESTDNGASW
                     QNIGEGIRAEYVPPDQAGDPGIQDPHRIAWSVSNPDTIWMQHHNGIFVSHDGGKNWCE
                     ITNAAPSAFGFAVAVHPHDPQTAWLIPGVKDECRVACDGALCVMRTQNGGETWEQLRA
                     GLPQQHAYHLIFRHCLDVSGDGRTLAFGSTTGSVWISEDGGDSWNRLSAELPPVYCVR
                     FGP"
BASE COUNT         1737 a         2926 c         2451 g         1579 t
ORIGIN      
        1 gccaccattc atcaagacgc cgacatctac cgcgtgcgcc ttccgaaggg ccagagcatc
       61 acccatgagg tcaaaaccgg ccgcggcgtg tggttccagc tcatcaaagg cagcatcacc
      121 acgcacggca gcacgctgac gcccggcgac gccttctcca ccgaggacac cggcacgctc
      181 accctcaccg cctccgatga cgccgaggcc ctcctcttcg acctcgcctg attcatcatc
      241 actccacaac tccaacactc caccctcatg aaacacgcat ccaccctcgc ctccgtcctc
      301 ctcggtctcg ccttcatcac gtttggcgcg aacttcttcc tcaacttcat cccgatgccc
      361 gccgatccat cgcccgctga cgcgccgcac aagctcttca tggccgcgat gttccccacc
      421 ggctacctcg ccttcgtgaa ggtgctggag atcctcggcg gcctgctcgt cgccctgcca
      481 aaaactcgta acatcggcct cctggtgctc gggcccatca tcgtgaacat cctcgccttc
      541 cacgtcttcc tcaccaaagg cgccggcctc accgatccgc ccatcctcgt catctcgatc
      601 ctctccgcct tcctcctctt caccgagcgc aaagcctggc tcggccttgt gaagtgatcc
      661 gatcatctca atctctccag cagtgcctcg gacacccacc agccttcggc cttcatggcg
      721 agcgcgagtt cgtaggcatc tgggagaact cctcgcctct tggcttccag aagaacgccg
      781 agcaatccgg tagttttgag gccaatatgg tgcgcagcag cccttcccaa agcttcgtcg
      841 agcaggagaa aatccgcctg catttccttt gccagagcga tggcttcgga ttcacccgga
      901 tcgagagtcg tttccaaaat ggctaccatt tcgcggtcgg tcgcgctgcg gctctcgatc
      961 cagtcacagc cgcccaccgt ttcagctcca gcacgaccac gaccatccac caccaactca
     1021 tgccacaccg ccacaggcac ataaatccgt ccgtaaagac tctggagcaa ggtcagcctt
     1081 cctacacggc tcaatgcaat cagtggagat catcagcgac aacaatcatg ccggcaaacg
     1141 cagttgtgag agcatttccg cgtccttcac catctgctcc tcatctccga agatggatat
     1201 ctgccgctgg cctagcaaag gaacaaaatc tcccttttcc atcccggcaa accttgccgc
     1261 gcgtccgagg gaaagcctgc gctgttcgaa aagatggatc gccatctcaa gacgcaactc
     1321 atccaaagtc atccctgccg cattcaagac atcggcaggc aattgcaaac tgatcatgcc
     1381 actcatggcc ttattctagc acacatcctc tttgtatgtc cacccccatg accgcccacg
     1441 agctccaaac ccttctttcc tccgcggatg cgccgttgct cgtgcaggtc ctgccggagg
     1501 aggttttcgc cgcgagacgc attccaggct ccaagcaggc ctgcgtgtat gaagtggctt
     1561 tcttcgatca aatcgccgct ctcacgtctg acaaagctcg cgccatcatc gtgtatggcg
     1621 cgggcagcgg ctcgctcgat gccaaagtgg cagccgaaaa gctcgtcgca gccggtttca
     1681 cgaacgtgag cgcctatgac ggcggtctcg atgcgtggag cgctgccggt ttgccgctcg
     1741 aaggtgaagg cacattgcca gccgcgcctt cgctcagcgg caccttccgt gcagacacgg
     1801 aacaaagcct catccgctgg accggtcgta atctattcaa ccaccacagc ggcatcgtgc
     1861 gcctcgccga cggcgaaatc acgctgcgcg acaacgcgct cgtggccgca cgcttccgca
     1921 tcgcgatgga cagcattgct tgcgaagaca tcagcgactc cgcgatgaac cagatgctca
     1981 tcgcgcacct gcacagcgac gacttcttcg atgtcgcgca tcatcccgtg gccgaattcg
     2041 tcgcggaaag catcacaccg ctcgccacct gcaccgacgg cacgccgaac tacctcctgc
     2101 gcggccagtt cactttgcgc ggcatcaccc gcgagatcga gtttcccatc ctcgccgcca
     2161 ccgccgatgg aaaacgcctc accgcgcagg gcgtgctcga tctcgaccgc acccagttcg
     2221 gcagcgttta cggctccggc cgcctcttcc gcttcctcgg caaacacatc gtcaacgacc
     2281 acgtccacct gcacatcaag ctccacgccg acctccaatc gtgacacaga cactcctgtc
     2341 tgtgccctat caaaaacagg caagagtgcc tgtttcaccc tttcccaaaa catcactcca
     2401 aaactccacc actccatgca cctcctctcc ccgctcaccg ccggcgctct gcaactcccc
     2461 aaccgtgtcc tcatggcccc gctcacgcgt tgccgtgccg atgccgatca caatcccacg
     2521 ccgctcatgg ccgaatacta cgcgcagcgg gccagcgccg gtttgatcat cgccgaggcc
     2581 acgatggtca tggagggcaa ttcctccttc tggatggagc ccggcatcta ctccgatgct
     2641 caaatcaaag gctggaaagc cgtcaccgat gccgtgcatg agaaaggcgg ccagatcgtc
     2701 ctccagctct ggcatggcgg tcgtgcctgt catccgctgc tcaatggcgg agcccaacct
     2761 gtggctccga gcgccattcc gatcaccggc gatgaagttc acacgcccga aggcaagaaa
     2821 ccctacgtca caccgcgtga actccgcgac gacgagatcc ccggcatcgt cgccggattc
     2881 aaaaaagccg ccgagaacgc caaagcggcc ggttttgacg gcgtcgaggt ccacggagcc
     2941 aatggctacc tgctcgacga attcctccgc gatggctcca acaagcgcag cggcccttac
     3001 ggcggctcca tcgaaaatcg cgcccgtttg atgctcgaag tgctcgatgc cgccatcagc
     3061 gtctggggcg cggatcgtgt cggtttgcgc atctcgccgc tcaacagcta caacagcatg
     3121 ctcgacagcg atcccgtcgc catcaccacc tacatcgcgc agcaatgcag cacgcgcggc
     3181 atcgcttacc tgcatgtcat gcgcagcgac ttcttccaag cgcagcaggg cgatgtcctc
     3241 acgccggcgc gacagcgatc ccgtcgccat caccacctac atcgcgcagc aatgcagcac
     3301 gcgcggcatc gcttacctgc atgtcatgcg cagcgacttc ttccaagcgc agcagggcga
     3361 tgtcctcacg ccggcgcgac agcatttcaa aggcacgctc atcagcaaca tgggctacac
     3421 cgccgacgaa gccgaacaag ccatcaccga aggcaaactc gacgccgtcg ccttcggcac
     3481 cagcttcctc gccaatcccg acctgcccgc ccgcatcgcc gcgaaagctc cgctcaatgc
     3541 gccgaacccc gcgaagttct actcccccgg ccccgagggc tacacggact atccgtttct
     3601 cctggctgtt tgattcatat gcgcggctct tgctcgcgac accctgccga aatccgccgc
     3661 ttttggccgc atccgcgccg aggtcatccg ccttgcagcc ttgtcccgga agacatgtca
     3721 ttcggcggcg gccgcctttt ttgcatcgcg cttgtgacgc accacctcaa tgatgatggg
     3781 caggagggag accacgatga tgagcaggaa gacgagcttg agattgtttt tgatcaccgg
     3841 gatgctgccc aggaaatagc cgccgaggcc gaagctgacc acccagatga caccgcccgc
     3901 gaggctaaag gtgagaaagc gcgtgtagtg catgcgtccc agtccggctg tgaacggcgc
     3961 gaaggtgcgg atgatgggcg cgaaccgcgc gaggatgatg gccttgccgc catggcggac
     4021 aaagaaggcc tccgtcttca ccagatagtc gtttttgaac cagcgctgcc gcaccatcca
     4081 gccgccggcc ttgcggccga tccagtagtt caggttgtca ccgatgatgg ccgccgtgat
     4141 gagcaggggc aggatgatct ccaggcggat gaggtcctgc gcggccagcg cgccgacggc
     4201 gaagagcaga gagtcgccag gcaggaaggg cgtgaccaca agacccgttt cacagaacac
     4261 aatcgcgaaa agcaggatgt aaatgagcgt gccatactcc tgggcgaagg cctggaggta
     4321 tttgtccaca tgcaggatgt aatcgagaag ctggctcatg aaggcgcgca tagtccgcct
     4381 tgggcggcgg cgggcaaatg aaatgtccgc gcgtcattcc ggcagatcct cgcccctggt
     4441 ttcgggcagc agggccagga agacaagccc aagaaggaaa aagccgccca ggcaggtgac
     4501 cgcgactcgc agatcaaagg cggccttcat ccacgcgctg agccagatga gaacaggagc
     4561 ggccagaatg cggcccgtgt tgaagcaaaa accggagccc gtggcgcgca gatgcgtggg
     4621 aaacagcgcc gggaagtagg ccgcgtaacc ggcatgaatg ccctgggcga aaaagccgaa
     4681 caccggcagc agcaccagca gcagcgcgta atttccaaaa gttgacggaa cccagcaaac
     4741 gacgggtgtc atcaccagcg cggcaaggtg catgacgaca aaggtctttt tgcatcccca
     4801 gcgggcgctg agagggccga aagccagcat gccggccccc gctcccgccg tctggatgaa
     4861 gccgaaggcg attttcgatt tgctggccca ttccgggtca ccgagctgct tgagcaaatg
     4921 ctcggcgatg ttttgacccg ccaccacgac accccaaaat gtggccaggc ccaccatggc
     4981 cagcagggcc ccaaacactg ctcttgagcg ccagcgcggg tctcctagca attcggcaaa
     5041 gctgcccagc cggtcggatt tctgctcgcg cgcctgcttc catgcctccg gctccttgat
     5101 gctgaggcgg acccagagca cgagcaacgc cgggatcacg cccaagaggt aggctgtgcg
     5161 ccattccgtg cccacccata agcctgccgc tgccgccagc cagaggcccg ccacactcgt
     5221 ggcatgaaaa atcccgcccg cacgctcgcg cgcctgcttc ggaaacacct ccgccacaag
     5281 cgccgctccc actgcccact cgccgcccac acccattgcc accaaaaagc gcagcacacc
     5341 cacatgccat gcctcagtgg cgaagtaggt cagtccggaa aagatggagt aaaacagaat
     5401 cgtcaacgcc agcaccggat tgcgcccgat gcggtccgcc atggagctga acagccagcc
     5461 gccaatggtg ccgccaatga ggaagatgcc gagaaaacgc tctccccatg ccttcaccaa
     5521 cggatcgtcc gcgcctactt tgaggatgtc aggcagcatg tcaccgcggg tgatattgta
     5581 gagctggcct tcaaatgcgt caaacaccca ccctaacgag gcgacagtca gaacgagcca
     5641 ttgatagcgt gtgacaccgg agtaccattt gggggatgtg gtcatgattg ggaggaatgg
     5701 cgggggagga aatctgaagc acgcgggaat gtcgaagcaa aaatgccaga ggcggctatc
     5761 aaacacgtca cgccatgccc gcttcttcct tcccgccgaa ccatccacgc atccgcgtgg
     5821 ttggcgcctt tgacgaactg gtcacagcgc gcttcgagag tggagtgaac gcgctgtgct
     5881 ggccgcgcgt gctggaggga gattttgagg aggttgtcat gaaactgaaa gcggggccgg
     5941 ggatcacgca tttgacggag gacatgctgc gcgccctgcc gctaagcgcc gccgggcgtc
     6001 tagcggcggc ggccatgctc gatgattatc aacgccttgc ggatcacggc ctgcagccag
     6061 cgctggactg cgtcaacggt tatgagcatg cggaggaaac cggacctgtg cgcacggatg
     6121 tctgctcctg gcatgcggac agcgccaccg ctgaagtgga cacctggctc tgcacctatc
     6181 acggcgcgtc cacggagggt ttgatcaacg aggaggctat ccgtcgtgtg gatgtgccgg
     6241 agacgcgcgc ggaactgctc aaattatacg gcggcgccga tgatggggac tttcgtgaat
     6301 ggctgaacga acgcttttac gatctgcact acgctcccct ccctcacgcg cggccgtatg
     6361 cgttcggcgc cggaaatctg tggcgcatcg ccacgctgca tgacagctgc gcggtgcccc
     6421 cgtgtgttca tcgcgcgccg ggttttgttc ccggtcagaa gcgcctcttg ctcatcagct
     6481 gatcaaacgt cttcctgccg gcgggttcga atcttcctgg cgtcaaaccc gcggcttgaa
     6541 ctacaacggc agcgtggccc ccggaaccgc cacgggatac cagccatccg cgtccggctt
     6601 caccggcggc tcgctgtccc aggtgaggtt gtctggcgcg aggttctccc gcgagttcat
     6661 gacctggtcc caggtgatga cctggccggt gtaggtggcc atgcggccca tcacggccat
     6721 gagcgtgctg tgcgcggcgt attcggcgtc gttgcggtaa ccgccggtgc ggatgttttc
     6781 aaagaaggca tcgtgctcga tctggtaggc gtcggttttg ttgcgcgcgc gtttgccacc
     6841 gggaggctgg ccgttgatga ggaaacgccc gttcgcggcg aggttggccg tgcctttgag
     6901 gccgtggaaa tgctcgctca catcacgctg gcatttgccg ccaatctggc agcactggct
     6961 gagcaggcga aagccgccct cgtattcaaa ctcgacggtg tggtggtcaa agatggtgcc
     7021 gttttccttc gcgcggcgca cctgccgccc gccctggccc gtggcgcgca cgggcatgtt
     7081 gccctgaagc acccagttgg ccacgtcgag attgtggcag tgctgctcca ggatgtggtc
     7141 gccgctcatc caggtgaaga agtaccagtt gcggatctgg taatgcagct cgctctcgcc
     7201 cggctcgcgc gggaagcctg ggcgcgagga gccgttccag tacacacggc catacacgag
     7261 gtcgccaatc tctccggcgt ggatgcggtt gatgacatac atgtaggccg gatcgtagcg
     7321 gcgctggaag ccgacgcaga cattgagctt cttctttttc gcctcctgcg cggcggcgat
     7381 cacgcgcctc gcgcccatgg catccaccgc gaccggcttt tccatgaaca catgcttccc
     7441 ggccttgacc gcctgctcaa agagaaacgg ccgagggccc ggaggtgtcg tgatgagcac
     7501 cacatcagcg agagcaaagg ctttctggta gtcctcgaag ttcgtgaaca cctggtcagg
     7561 cggcacatcc acctgcccct cgtgtttttg tttgaggatg tcgagcgcgg cctgcgcctt
     7621 gccctccagc ggatccacca ccgccaccag cttgatggtg gagccggcgg acagcgcctg
     7681 attacacgcg cccgtcccgc gcccgccgca gccgatgagg gcaattttga tgtcactgct
     7741 gccaccggac gcaaaagcgt gtgtggcggc ggaaagcgcc agcccggaag tggtggcgag
     7801 aaaggatcgg cgggtgctca tctcccctca actgccggag tcgcgcccat atttcatggc
     7861 ccaaaccgca cgcaatacac cggcggcagc tcggcgctga ggcgattcca actgtcaccg
     7921 ccgtcttcgc tgatccacac gctgccggtg gtgctgccaa aggcgagcgt gcgaccgtcg
     7981 ccactgacat cgaggcagtg gcggaagatg aggtggtagg cgtgctgctg cggcaggccg
     8041 gcgcggagct gctcccaggt ttcgccgccg ttttgagtgc gcatgacgca cagcgctccg
     8101 tcgcaggcca cacgacactc atctttgacg cccggaatga gccacgcggt ctgtggatca
     8161 tgtggatgca ctgccacggc gaaaccgaag gcagacggcg cggcattcgt gatctcacac
     8221 cagtttttgc cgccgtcgtg gctgacaaaa atgccgttgt gatgctgcat ccagatcgtg
     8281 tcgggattcg agacactcca ggcgatgcgg tgcggatctt ggatgcctgg atcgccggcc
     8341 tgatcgggcg gcacatattc ggcgcggatg ccctcaccaa tgttttgcca gctcgcaccg
     8401 ttgtcggtgg attcccacac accagcacag cttacagcga tcagcaggcg ctgtttgtcg
     8461 cgcggatcaa tcaggatgct gtgaatgccc ggccagtccg cgccgccgcc aaaccacttg
     8521 gcacgatccg gatgattcca gaggctctct accagcctcc acgacgcgcc gtggtcggtg
     8581 gaatgaaaca aaccgccggg aatcgtgccg caccaaagca caccttcgcc cgcgccagct
     8641 tcgagcgccc aaatcttctt caacgaccat ggagttggct cctggctcat cgg
//
LOCUS       DAOMYJ010000216         8682 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Prosthecobacter sp. isolate AMR_MDS_5629
            NODE_2701_length_8682_cov_23.838298, whole genome shotgun sequence.
ACCESSION   DAOMYJ010000216 DAOMYJ010000000
VERSION     DAOMYJ010000216.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867850
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prosthecobacter sp. (wastewater metagenome)
  ORGANISM  Prosthecobacter sp.
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Verrucomicrobiaceae; Prosthecobacter.
REFERENCE   1  (bases 1 to 8682)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8682)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.30
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:34:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,742
            CDSs (total)                      :: 4,684
            Genes (coding)                    :: 4,676
            CDSs (with protein)               :: 4,676
            Genes (RNA)                       :: 58
            tRNAs                             :: 55
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8682
                     /organism="Prosthecobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2701_length_8682_cov_23.838298"
                     /isolate="AMR_MDS_5629"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1965333"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..486
                     /locus_tag="PLP58_17820"
     CDS             <1..486
                     /locus_tag="PLP58_17820"
                     /inference="COORDINATES: protein motif:HMM:NF019223.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1553 domain-containing protein"
                     /protein_id="HRJ10443.1"
                     /translation="LYGPPSPVSVREDGLIMEQERPGGMFRRSLYLRLRRTEMPSMLA
                     TFDYPEMQPNCVQRSISTVSLQPLMLQNNARVRELAAAFAARLETASGDDPTVIRLAF
                     ETALQRPPDDAETADSLAALRQLTAAWQETGAIPAEARRKALASFCHTLLNSAGFLYL
                     D"
     gene            489..893
                     /locus_tag="PLP58_17825"
     CDS             489..893
                     /locus_tag="PLP58_17825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ10444.1"
                     /translation="MTADENSWSVTARRFPRLRQHQQWALIFLVGIFLMQDSAAAFFE
                     TAGILTLIGLWLWLRRPGCLRVQPSGITFPCGLFIPAAEIEEISPSPAGWQIAFQHRG
                     VLRFVEVREACFDDAEWPRVRAAFRTWHRTCS"
     gene            872..2296
                     /locus_tag="PLP58_17830"
     CDS             872..2296
                     /locus_tag="PLP58_17830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ10445.1"
                     /translation="MAQDLLMRPLRESPGLPANPRKRLPAWRLGFILLIIFLACIQWG
                     MVRSRHPTVVLPDGVRVSYYGSSHGVTMGTSNFHHTGPPAEEYHPFWLHWLRSSLPPS
                     ISSHLPAPIMHRTGYPAWPDMHEFELQFQTKGNLSQHPWDIFVADEHGWETRVRDYTW
                     SYNGAAKSTGEAVSGWLRLGSAYPRRSKTIRIVFHPNDRQTPKPDRETRLKPAAQMIV
                     PNPFWQASSSNVAEPLPLSLPFRGETLTLKELRRDRIVGDGYGALHSLLVVERDGKPL
                     PGFEVARMSVSDSSGQVFRQTTGGSSESSGRMWNSIDTAPWSDDPCWLVRYSLCRKAY
                     SLDVPEDEQFKFEGLPLPSEAPVNFERKVTCRGMTLSLGKFTQASDAPRFLVSGLAND
                     ITNGKRIILLDARDDQDRTETSDPRFGRRKIVGGPVGGSQEGMTYSLNLPADAKSWSL
                     TLLVETLTEVEFRVRSPLANTPPK"
     gene            2397..3884
                     /locus_tag="PLP58_17835"
     CDS             2397..3884
                     /locus_tag="PLP58_17835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009960998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ10446.1"
                     /translation="MNRASLSFLAALAVLAGVVLISGSGDPAGREESSSSPQPPDAGL
                     RVAVFQADATPPIGSPVAYAPARSIEDPLSARGVVLLGAGEPVVLCAVDWIGIDNEGH
                     DAWRQALAEAAGTTVERVAVHVLHQHDGVRGDFGAEALLEAAGLAGIRCDASFVRRAI
                     AAAAGALRASLADARRVSHLGVGEARVEKVASNRRILGSDGRVMIARSSSYRIPEPLL
                     SRLAENARRNGYDLSASRVDEALAAPEGVIDPMLKMLTFYEDEEPLASLSYYATHPQS
                     YFGKGDVTYEFVGLARARHEHANGGLTLVHFNGAAGNIAAGKYNDGSPEARVRLTDRM
                     ADGMRRAFAAAKKVPLQAGDVGWKVVPVSLPLASHLDADALRAKIMDTSAEEGERLAA
                     AGKLAFVQRMKSGCRIDLACLRLGSVLILHMPGELFVEYQLAAQKLRPEAVVCMAAYG
                     EYGTGYIGTKAAYDEGGYETQPSSSNVAPQVEEVLMRGIRALLAD"
     gene            4069..5487
                     /locus_tag="PLP58_17840"
     CDS             4069..5487
                     /locus_tag="PLP58_17840"
                     /inference="COORDINATES: protein motif:HMM:NF019040.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1501 domain-containing protein"
                     /protein_id="HRJ10447.1"
                     /translation="MLTRRSFFQNMSDGLCGAALAHLFAGDRLWGAAAQARHDLSPKS
                     AHFPARAKAVIHLFMNGGPSQMDLFDPKPALEKHAGKSFPGNIEELGNVGTSNVGAIM
                     PPVCKFARHGQSGIWMSDALPHTARVVDELCFIHSLWTDHPNHDNALYKIHTGRLFMG
                     HPSLGSWVAYGLGSECKDLPAYVVLDDPLGLPKNDVRNWTSAYLPQVYQGTRFRSVGA
                     PILNLTPENEEPSAVSQAARDLLERLDGRHRAQRPGYGELDARIEAYSLAARMQLSAS
                     DVLDLDQESEATRAAYGIGQKITDSYGRRCLLARRMVERGVRFVQIFMEDQPWDNHSN
                     LEGTLRSACERTDQPVAALIKDLKQRGLLDDTLVIWGGEFGRTPTSQKIGNDFSGRDH
                     NMQGFTSWMAGGGLKKGFVYGETDELGHKAVVNPVSVPDFHATILHALGLDAERLHFE
                     RNGLEERLTGFTTPKVAKAVFA"
     gene            5539..6423
                     /locus_tag="PLP58_17845"
     CDS             5539..6423
                     /locus_tag="PLP58_17845"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HRJ10448.1"
                     /translation="MSAPVIVFPFLAALMYALGALTLKRSSELGVGLWRTTFVANVIV
                     ALLFSALWLFGGPPVERELLWQPGVIAMCLFGGQIAQFLALEKGDVSIAVPVFGLKVV
                     IVAMITPMLTGENVDPRLWAACILSVLGITFLNRNDGGPRPRGLGITLAAGGVGAVCF
                     AVFDVLVQKWGPHWGAGRLLPCVFWINALLSFGLVFRFSAPLSAVPRAAWPWLCGGSA
                     LLGLQSITFVSTLAIHGQAAAANVVYAARGLLSVALVWMVGHWFKNTEQHLGARVLRW
                     RMAGALMMMSAIVLVVLR"
     gene            6471..8660
                     /locus_tag="PLP58_17850"
     CDS             6471..8660
                     /locus_tag="PLP58_17850"
                     /inference="COORDINATES: protein motif:HMM:NF019223.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1553 domain-containing protein"
                     /protein_id="HRJ10449.1"
                     /translation="MPRLPPAITLWLTLSLTGHALAEEKPVSFIHDIMPRLMKAGCAG
                     GNCHAKPEGQNNFKLSVFGYDPAHDYHEIVRDARGRRVFMAAPEESLLLRKAAGQMPH
                     EGGARIEKGGPAWNLLVTWIKQGAPFALEGEPSLTHVSVQPREGSYAKGAAQQLTVTA
                     SYSDGATRDVTSLTDYLSQDKELAEVDEQGLVKTGQISGEGVIVVRFMGHVDVARFTL
                     PSDKKLPAEIYAELPVNNEIDRLVHARHEKLGLLPSGLCTDEEFLRRASLDLIGKLPS
                     AELARRYLADTRPGKRARLVDELLEDPNYADHWAVKWGDLIRPNPSRVGVKPVYLLDS
                     WLRESFRQNKPYDRMIAELLTAQGSSHQHGPVAVFRDKRDPADMGAFVSQIFLGVRLD
                     CAKCHHHPSEKWTQDDYYQLAAFFGRMKRRGQAISAPISGEVEYWWHGGTGSVTHPVT
                     EAVMTPKPPDGPEMPYVEGQDPRARLADWMRASDNPFFAKALVNRIWSDFLGRGIVEP
                     VDDFRVSNPPSNPALLDWLAADFISSGYDLKHLMRTIAASRTYQLSVEPNEHNLADTR
                     NFARAHKRRLSAEVLLDAVGDLTGIRDTFQGLPPNSRAVQTWNHKLSSEFLDAFGRPN
                     ASQECPCERERKSSVVQALHLMNSDSLQNKLASEKGRVTALVKDASLSEPKLVTEIYL
                     AAYNRLPEAGEMQAALRFFSTPGATRQTAAEDLAWALINSAEFVFNH"
BASE COUNT         1528 a         2919 c         2638 g         1597 t
ORIGIN      
        1 ctctacgggc cgccctcgcc cgtctccgtg cgcgaggacg gcctcatcat ggagcaggag
       61 cggcccggcg gcatgttccg ccgcagcctc tacctgcgcc tgcgccgcac cgagatgccc
      121 tccatgctcg ccacctttga ttatcccgag atgcagccca actgcgtgca gcgcagcatc
      181 tccaccgttt ccctccagcc cctcatgctg caaaacaacg cccgtgtccg cgaactcgcc
      241 gccgccttcg ccgcgcggct cgagaccgcc tccggcgacg atcccaccgt catccgcctc
      301 gccttcgaga ccgccctcca gcgccctccc gatgacgctg agaccgccga ctcccttgcc
      361 gccctgcgcc agctcaccgc cgcctggcag gaaactggcg ctattcccgc cgaagcgcgc
      421 cgcaaagccc tcgccagctt ctgtcacacc ctgctgaact ccgccgggtt tctttacctg
      481 gactgaacat gactgcggac gagaattcct ggagcgtgac cgcgcggcgg tttccaaggc
      541 tgcggcagca tcaacagtgg gcgcttatct tcctggttgg catctttctc atgcaggatt
      601 cagcagcagc cttttttgag actgccggca ttctgacgct catcggcctg tggctttggc
      661 tgcgccgtcc cggatgtctg cgcgtccagc cttcgggcat caccttccct tgcggcctct
      721 tcataccagc ggcggagatc gaggaaatca gcccgtctcc cgcaggctgg cagatcgcgt
      781 tccagcaccg tggcgtgctg cgttttgtcg aagtgcggga ggcatgcttt gacgacgccg
      841 aatggccgcg tgtgcgcgcc gcatttcgaa cgtggcacag gacctgctca tgagaccgtt
      901 gagggaatca ccaggactgc cggccaatcc caggaagcgt ctgcccgcat ggcggctggg
      961 tttcatactg ctgatcatct tcctggcttg tatccagtgg ggcatggtta gatctcgtca
     1021 cccaaccgtt gttttgccag atggagttcg tgtcagctac tacggcagca gtcatggcgt
     1081 gaccatgggg acatccaatt tccaccacac tggaccaccg gcggaggaat accatccctt
     1141 ctggctgcac tggctgcgga gttcactgcc accttcaatc agcagccatc ttcccgcccc
     1201 catcatgcac cgcacgggct accccgcctg gccggacatg catgagtttg agcttcagtt
     1261 tcaaacgaaa ggtaatctga gccagcaccc ctgggacatc tttgtggcag atgagcatgg
     1321 gtgggaaacc cgggtgcgtg attatacctg gagctacaat ggagcggcaa agtcaactgg
     1381 agaggctgtg tctggctggt tgcggcttgg cagcgcttac cctcgccgat cgaaaacgat
     1441 tcggatcgtg ttccatccca atgatcgtca gacaccaaag ccggacaggg aaacacggct
     1501 aaaacctgcc gctcagatga tcgttccgaa tcccttttgg caggcttcat catccaatgt
     1561 tgcggaacct ttgcctctgt cactgccctt tcgcggggag actctgactc tcaaagagct
     1621 tcggcgcgac cgcattgttg gagatggtta tggggcgctc cattcattgc tcgtcgtcga
     1681 gagagacggg aagcccttgc caggttttga ggtggcccgc atgagcgtca gtgacagttc
     1741 tggacaggtg tttagacaga caacaggagg ctccagcgag tcttcgggac ggatgtggaa
     1801 cagcatcgac acggcgccgt ggagtgatga tccctgctgg ctggtgcgct acagcctgtg
     1861 tcggaaggcg tattccctcg acgttcctga ggacgagcag ttcaagtttg aagggttgcc
     1921 cctgccctct gaggctccag tcaatttcga acgcaaagtc acatgccgtg gaatgacgct
     1981 aagtcttggg aaattcacgc aggcaagcga tgctccgcga ttcttggtgt cgggcttggc
     2041 aaatgacatc acaaatggaa aacgaatcat cctgctggat gctagagatg atcaagaccg
     2101 cacggaaacg agcgatccac gctttgggcg acggaaaatc gtgggtggac cagtgggcgg
     2161 ctcgcaggaa ggcatgacct acagcctgaa ccttcctgca gatgcaaaat catggagcct
     2221 caccctgctt gtcgagacat tgaccgaagt agagtttcgc gtgcgctcac ctttggcaaa
     2281 tacaccgcct aaatgaacgt tcgcgcattg tgaaacacaa tgcgggccaa agttgatgaa
     2341 aaacatcatg ccgcccggct ggatggaggc ggttgagccg cgtatgattt gtcatcatga
     2401 atcgcgcttc gctttcgttc cttgccgctc ttgctgtgct ggcgggtgtt gttttgatct
     2461 cgggctcggg ggatccggca gggcgtgagg aaagctccag ctcgccgcaa ccgccggatg
     2521 cggggttgcg cgtggcggtg tttcaggcgg atgccacgcc gccgatcggg tcgccagtgg
     2581 cctatgctcc ggcgcggtcc attgaagacc cgttgagcgc gcgcggggtg gtgctgctgg
     2641 gggcgggtga gccggtggtg ctgtgcgcgg tggattggat cggcattgac aacgaggggc
     2701 atgacgcatg gcgccaggct ttggcggagg cggcaggcac cactgtggag cgcgtggcgg
     2761 tgcatgtgct gcaccagcac gacggggtgc gcggtgattt cggcgcggag gcgctgctgg
     2821 aggctgctgg gctggcgggc atccgctgtg acgcgtcttt tgtgcggcgg gccatcgccg
     2881 ctgccgccgg ggctttgcgc gccagtctgg cggacgcgcg cagggtttct cacctgggcg
     2941 tgggcgaggc gcgcgtggag aaagtcgcct ccaaccgccg catcctcggg agcgacggca
     3001 gggtgatgat cgcccgctcc agctcctacc gcattcccga gccgctgctc tcccggctcg
     3061 cggagaatgc ccggcgcaat ggctatgacc tgagcgcgtc gcgcgtggat gaggcgctgg
     3121 ccgcaccgga gggggtgatt gatcccatgc tgaagatgct gacattttac gaggatgagg
     3181 agccgttggc gagcctgagc tactatgcca cgcacccgca aagttacttt ggcaaaggtg
     3241 atgtcaccta cgagtttgtc ggcctggcgc gcgcgcggca tgagcatgcg aacggcggcc
     3301 tcacgctggt gcatttcaac ggagccgcgg gcaacatcgc cgccgggaaa tacaacgacg
     3361 gctcgccgga ggcccgtgtg cgtctgaccg atcgcatggc ggacggaatg cgccgcgctt
     3421 ttgcggccgc aaaaaaagtg cctcttcagg ctggcgatgt cgggtggaaa gtcgtgcctg
     3481 tcagcctgcc cctggccagt catctggatg ccgatgcgtt gcgggcgaaa atcatggaca
     3541 ccagcgccga ggagggcgag cgcctcgccg ccgccggcaa actggccttc gtgcagcgca
     3601 tgaaaagcgg atgcagaatt gatctcgcct gtctgcggct ggggtcggtg ttgattctgc
     3661 acatgccggg cgagttgttt gtggagtatc agcttgccgc gcagaagctg aggccggagg
     3721 cggtggtctg catggcggcc tacggcgaat acggcaccgg ttacatcggc accaaggctg
     3781 cctatgacga gggcggctat gaaacgcaac ccagctcctc caatgtggcc ccccaagtcg
     3841 aggaggtgct catgcggggc attcgcgctc ttttggcgga ctgacaggcg gggcagggtg
     3901 tgagagcggg acagggcgct tgcaaagcgc cactccttgg gcattcgcgc ctgcggtgct
     3961 ggatcagcgc gcgcttccct tgagagcggg cttcaaggaa tgacgttttg ttgtctcgaa
     4021 aatggccgag tgctgctcaa atgccgctcc cggcttcgta cacccgccat gctcacacgc
     4081 cgctcctttt tccagaacat gtccgacggc ctctgcgggg ccgctctggc gcatttgttc
     4141 gcgggcgacc gcctctgggg cgcggcggca caggcgcggc atgatctgag cccgaagtcc
     4201 gcgcattttc cggcacgggc caaggcggtg atccatctgt tcatgaatgg aggtcccagc
     4261 cagatggacc tcttcgaccc caaaccggct ctcgaaaaac acgccgggaa gtccttcccc
     4321 ggcaacatcg aggagctggg caatgtgggc acctccaatg tcggcgctat catgccgccg
     4381 gtgtgcaaat tcgcccggca cgggcagtcg ggcatttgga tgtcggacgc gctgccgcac
     4441 acggcgcggg tggtggacga actgtgcttc attcactcgc tgtggaccga ccacccgaac
     4501 cacgacaacg cgctttacaa aatccacacc ggccggctgt tcatgggcca cccttccctc
     4561 ggctcctggg tggcctacgg cctgggcagc gagtgcaagg acctgcccgc gtatgtcgtg
     4621 ctcgacgacc cgctcggact gccgaaaaac gacgtgcgca actggacctc ggcctacctg
     4681 ccgcaggtgt atcaaggcac gcgcttccgc agtgtcggcg cgcccatttt gaatctcacc
     4741 ccggagaacg aggagccgag cgccgtcagc caggccgcgc gggacctgct ggaacggctg
     4801 gacggacgcc accgcgctca gcgccccggc tacggcgagc tggacgcgcg catcgaggcc
     4861 tacagcctgg ccgccaggat gcagctcagc gccagcgatg tgctggattt ggatcaggaa
     4921 agcgaggcca cccgcgccgc ctacggcatc ggccaaaaaa tcaccgactc ctatggccgc
     4981 cgctgcctgc tggcgcggcg catggtggag cgcggcgtgc gctttgtgca gatcttcatg
     5041 gaggaccagc cgtgggacaa tcacagcaac ctcgaaggca ccctgcgctc cgcctgcgag
     5101 cgcaccgacc agcccgtggc cgcgctcatc aaggacctca aacagcgcgg cctgctcgat
     5161 gacacgctcg tcatctgggg cggcgaattc ggacgcacgc ccacttcgca gaagatcggc
     5221 aacgacttca gcgggcgcga ccacaacatg cagggcttca cgagctggat ggccggcggc
     5281 ggattgaaaa aaggcttcgt gtatggcgag acggacgagc tcggccacaa agccgtggtc
     5341 aatcccgtca gcgtgcccga tttccacgcc accatcctgc acgcgctcgg cctggacgcg
     5401 gagcgcctgc acttcgagcg caacggcctg gaggagcgcc tcaccggctt caccacgccg
     5461 aaagtcgcga aggcggtttt tgcctgaggc cagccgcctc cttgaccccc ggcgcttcca
     5521 cgccagtctg gccgcctcgt gtccgctcct gttattgttt ttcctttcct agccgccctc
     5581 atgtacgccc ttggggcgct gaccctgaag cggtcgagcg agttgggtgt ggggctgtgg
     5641 cgcaccacct tcgtggcgaa tgtcatcgtc gcgttgctgt tctctgcgct ctggctcttc
     5701 ggcgggccgc cggtggagcg tgagctgctg tggcagccgg gcgtcattgc catgtgcctg
     5761 tttggcgggc agatcgcgca gtttctggcg ctggaaaaag gggacgtgtc catcgccgtt
     5821 ccggtctttg ggttgaaggt ggtcattgtg gcgatgatca cacccatgct cacgggtgaa
     5881 aacgtggacc cgcgtctgtg ggcggcctgc atcctcagcg tgctgggcat cacctttctc
     5941 aatcgaaatg acggcggccc aagaccgcgc ggactgggca tcacactggc ggcgggcggc
     6001 gtcggcgctg tgtgtttcgc tgtgttcgat gttcttgtgc agaagtgggg gccgcattgg
     6061 ggcgcgggcc ggctgctgcc ttgtgttttt tggatcaacg cgctgctttc tttcggtctt
     6121 gtcttccgtt tttccgcccc tctgagcgct gtgccgcgcg ccgcctggcc gtggctctgc
     6181 ggcggttcgg cgctgctggg actgcagagc atcacttttg tcagcaccct ggccattcac
     6241 ggccaggccg ccgctgccaa cgtggtttat gccgcgcgcg gtttgctgag tgtggccctc
     6301 gtctggatgg tagggcactg gttcaaaaac accgagcagc accttggcgc gcgcgtcttg
     6361 cgctggcgca tggccggcgc gctgatgatg atgagcgcca tcgtgctggt ggtcctccgc
     6421 tgagcttcac ccacggcgca agacccgtct cacgcggccg tattgtgccc atgccgcgcc
     6481 tgccgccagc catcaccctc tggctgaccc tgagcctcac gggccatgcc cttgccgagg
     6541 aaaaaccggt cagtttcatc cacgacatca tgccccgcct catgaaggcg ggctgcgccg
     6601 gcggcaactg ccatgccaag cccgagggcc agaacaactt caagctctcc gtcttcggct
     6661 acgaccccgc ccacgactac cacgagatcg tccgtgacgc gcgcggccgc cgcgtcttca
     6721 tggccgcccc ggaggaaagc ctgctcctgc gcaaagccgc cggccagatg ccgcacgagg
     6781 gcggcgcccg catcgaaaaa ggcggccccg cctggaatct cctcgtcacc tggatcaagc
     6841 agggcgcccc cttcgccctc gaaggggagc cttcactcac tcatgtcagc gtccagccgc
     6901 gggagggcag ctatgccaaa ggcgccgcac agcagctcac cgtcaccgcc agttactccg
     6961 acggcgccac gcgcgatgtc accagcctca ctgactacct ctcccaggac aaggaactcg
     7021 ccgaagtgga cgagcaagga cttgtcaaaa ccggacagat cagcggagag ggggtcatcg
     7081 tcgtccgctt catggggcat gtggatgtgg cgcgcttcac cctgccatct gacaaaaagc
     7141 ttcccgccga gatttacgcg gagctgcccg tcaacaacga gatagaccgc ctcgtccacg
     7201 ccaggcatga aaagctcggc ctcctgccct ccggcctctg caccgacgag gagttcctcc
     7261 gccgcgcctc ccttgacctc atcggcaaac tcccctccgc cgagctcgcc cgccgctacc
     7321 tcgccgacac ccgccccggc aaacgcgccc gcctcgtgga cgagctgctc gaagacccca
     7381 actacgccga ccactgggcc gtgaagtggg gcgatctcat ccgccccaac ccctcccgcg
     7441 tcggcgtcaa acccgtctat ctgctcgact cctggctgcg cgagagcttc cgccaaaaca
     7501 agccctacga ccgcatgatc gccgagctgc tcaccgccca gggcagcagc caccagcacg
     7561 gccccgtcgc cgttttccgc gacaagcgcg accccgccga catgggcgcc tttgtcagtc
     7621 aaatcttcct cggcgtccgg ctcgactgcg ccaagtgcca ccaccacccc agcgaaaagt
     7681 ggacacagga cgactactac cagcttgccg ccttcttcgg ccggatgaag cgccgaggcc
     7741 aggccatctc cgcccccatc tccggcgagg tcgaatactg gtggcacggc ggcaccggca
     7801 gcgtcaccca ccccgtcacc gaagccgtca tgacccccaa gccccccgac ggccccgaga
     7861 tgccctacgt cgaaggccag gacccacgcg cccgcctcgc cgactggatg cgcgccagcg
     7921 acaacccctt cttcgccaaa gccctcgtca accgcatctg gtccgacttc ctcggccgcg
     7981 gcatcgtcga gcccgtggat gattttcgcg tctccaatcc tcccagcaat cccgccctgc
     8041 tagactggct cgccgccgac ttcatctcca gcggctacga cctgaagcac ctcatgcgca
     8101 ccatcgccgc ctcgcgcacc taccagctca gcgtcgaacc caacgagcac aacctcgccg
     8161 acacccgcaa cttcgcccgc gcccacaagc gccgcctcag cgccgaagtc ctgctcgacg
     8221 ccgtcggcga tctcaccggc atccgcgaca ccttccaggg tctgccgcca aactcccgag
     8281 ccgtgcagac ctggaaccac aagctcagca gcgaattcct cgacgccttc ggccgcccca
     8341 acgccagcca ggaatgcccc tgcgagcgcg agcgcaagtc cagcgttgtc caggccctcc
     8401 acctcatgaa ttccgactcg ctgcaaaaca agctcgccag cgagaaaggc cgcgtcaccg
     8461 ccctggtgaa agacgcgtcc ctctccgagc caaagctcgt caccgagatc tatctcgccg
     8521 cctacaaccg cctgcccgaa gccggggaaa tgcaggccgc cctgcgcttc ttctccaccc
     8581 caggcgccac ccgccagacc gccgccgaag acctcgcctg ggcgctcatc aacagcgccg
     8641 aattcgtttt caatcactga cattcaccaa agtgccacgg ct
//
LOCUS       DAOMYJ010000217         8655 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Prosthecobacter sp. isolate AMR_MDS_5629
            NODE_2715_length_8655_cov_19.495581, whole genome shotgun sequence.
ACCESSION   DAOMYJ010000217 DAOMYJ010000000
VERSION     DAOMYJ010000217.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867850
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prosthecobacter sp. (wastewater metagenome)
  ORGANISM  Prosthecobacter sp.
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Verrucomicrobiaceae; Prosthecobacter.
REFERENCE   1  (bases 1 to 8655)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8655)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.30
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:34:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,742
            CDSs (total)                      :: 4,684
            Genes (coding)                    :: 4,676
            CDSs (with protein)               :: 4,676
            Genes (RNA)                       :: 58
            tRNAs                             :: 55
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8655
                     /organism="Prosthecobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2715_length_8655_cov_19.495581"
                     /isolate="AMR_MDS_5629"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1965333"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            178..966
                     /locus_tag="PLP58_17855"
     CDS             178..966
                     /locus_tag="PLP58_17855"
                     /inference="COORDINATES: protein motif:HMM:NF015648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PH domain-containing protein"
                     /protein_id="HRJ10450.1"
                     /translation="MRYLLHEHPAFPGLLSREDLFVLVERGSLRRGDLCTDTLTRRDH
                     TVGEVISGMKPPRGGTARVARPSFQEIRADLPHDPPPEDMEPDEAGDEETEAGDEDDE
                     GEDSEGDVEDGDDGGEETIQLHAHPSWLNYPGSFFLILLLLGGAGWLLAYGGEYALIP
                     LLAALAWLLGVAIARFSTDYMVTDERVEKVWGILGRSSKEVRICDIRSIDVYEKGLLG
                     LLGIGTVDVSSAGNAGIEVSFKNMRGAHAVKHLVRRLQRGSRAG"
     gene            1031..1558
                     /locus_tag="PLP58_17860"
     CDS             1031..1558
                     /locus_tag="PLP58_17860"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HRJ10451.1"
                     /translation="MHDPHHRFQELLTRHERPLIRYAHSITGNLEDARDVVQDVFVKL
                     SQNLHAIGEARLTSWLFTVCKNRALDHLRKHQRLIVMENETLDLETSPAPRPGDELDQ
                     RETASTLRALIDELPARQREAVRLKFIAGLDYKEISQAMQTSLGNVGYLIHHGVQALR
                     LKWREMEQGAGVARA"
     gene            1604..3949
                     /locus_tag="PLP58_17865"
     CDS             1604..3949
                     /locus_tag="PLP58_17865"
                     /inference="COORDINATES: protein motif:HMM:NF023459.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HRJ10452.1"
                     /translation="MKPEHITAWALDELPPAESAALEDALQENPQSLNEARDTRAFCD
                     LLTRELRDDSLALAPDQRSQLECLSEDGGMGHPACAGQAARLSPMQNGRVTLAQSKPH
                     HGWSRHGITGLAAAACVALLAWMGMKQEMGTRTLGEDGKDKEPPVEPNAATVTVTPGK
                     PQPEIRQKVNEKKRDGLVLTSPVRTDAESSSLVQAADFPSLPPAQPSFGKTPLSVPLE
                     RGAFGGGSPPMPAAAPAGPTPLFAFKNGSGTLNDTRDAVLPRTNTAAGEGEKKAADSF
                     SNLNAPADEARAFRRADNSNESYTRIIENSLLEVQREPLSTFSIDVDTASYANVRRFL
                     NQNTRPPADAVRIEELVNYFPAQEEGPPPGAEHPFGVKVEISSCPWQPRHRLARVHLQ
                     ARDIGRERPASNLVFLVDVSGSMSSDDKLPLVRRSLQMLAERLGENDRVSMVTYAGGT
                     QVVLPSVNGLQKADIIAAIDRLQSGGSTHGSAGIRLAYEQAVAGFIQGGVNRVILCTD
                     GDFNVGISDPAELEGFITEKARSGVFLSVLGFGTGNLQDRTMETLADKGNGNYAYVDS
                     LGEARKVLVEQMNGTLVTVAKDVKIQIEFNPAVVRQYRLIGYENRLLAKEDFNDDTKD
                     AGEIGAGHNVTALYELVPASLPPGEPARPLVDALRYQRQIASPPALAATPAAASGEAI
                     TVKLRYKEPDGGTSRLLEVPVRDTGADLRAAGGELQFSAAVAGFGLLLRGSSYAGQLT
                     WEQVRELALKGKGADPLGYRGEFIQLIEKARGLSGQDRAPP"
     gene            complement(3953..4162)
                     /locus_tag="PLP58_17870"
     CDS             complement(3953..4162)
                     /locus_tag="PLP58_17870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ10453.1"
                     /translation="MIPQTVDTLLTIEGAQSARTWHARLPNGRAVLAFRPEESAPLIL
                     EPGAKARARLTVCDFSRALVLADEE"
     gene            complement(4159..5307)
                     /locus_tag="PLP58_17875"
     CDS             complement(4159..5307)
                     /locus_tag="PLP58_17875"
                     /inference="COORDINATES: protein motif:HMM:NF015531.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HRJ10454.1"
                     /translation="MLSFPNAAPPVFSLAGKAAGLWLAAVFCQAAAQGAEPARGVDEL
                     SQGALQNAFQILRGEYIRGADLTHDELNRAAFQGLLDRLDLGAELAPRTEALPALTRT
                     GLLAELLADDILYLKPRSFDEGTAPAMEQKLRGHARTRHLILDLRSPAPPGEFELAAA
                     MLELFLPRGELMFKLKQPGRDDARLFLASREPVWTRPVILLADAETCNLGETLAAVLR
                     ARRQALVVGEKTRGATVRYESLPLDDAWLLRFARAEMLLADDTSFFKKGVPPDFPVRL
                     PAADKHRIFDARVTVGDTIFDTQVPRFNEAALLARKNPEIESYIRRSTGGESGEDAPL
                     LRDTVLQRAVDMLRASAHLEASKVKWPAARPEPAGPAAKKAPPAADVP"
     gene            complement(5411..5539)
                     /locus_tag="PLP58_17880"
     CDS             complement(5411..5539)
                     /locus_tag="PLP58_17880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ10455.1"
                     /translation="MTTFWLLLTAAALLWYSSVTLYVAVKGAGDIREMISRLKGRR"
     gene            complement(5536..7155)
                     /locus_tag="PLP58_17885"
     CDS             complement(5536..7155)
                     /locus_tag="PLP58_17885"
                     /inference="COORDINATES: protein motif:HMM:NF012686.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter family protein"
                     /protein_id="HRJ10456.1"
                     /translation="MPAPTHFHWLLDGGIVGIYLLVTMAAGLAMRRHVGKLEDYLVAG
                     REVRLHLGIASLAATEFGIITCMYTAQAGYTQGFSGAVPGLCQAAAMALIGLTGFCIK
                     PLREAGAMTLPEMFERRYGRAVRWLSGVVIVMGGLLNMGVFLQIGGKFLCTVAGFDLS
                     HLALLMTALLLMVVIYTVLGGMLSVLVTDYLQFIVMSAGLVVVSVLVLKEVGWDRVVE
                     SVRAHHGEGGFNPLANPSLGWPFLINQFIANTAAALTWQAVIVRVLSARDSATSRRIY
                     TRTSFFFVCRFLLPGIWGMAALAVLGWAPLKALNETDRAALPAAVAEKISASPVGQPL
                     AGLDTAELAAVSDEARARLADASLLALPEFLGGFLPIGLMGLLIAAMLAADMSTNSSY
                     MLSWGSVIYNDILAPFRRRPWPDARAIRWNRCIVALIGVYLLVFGLFYKIEGNVWSYL
                     LLTGSIYLSSMSVLLIACCYWKRANNWGALGGILLGALVPVAHLTLEKLPSTAAWAAS
                     VGKDIAGLAAFAAAALGMIAGSLLKPAPKAP"
     gene            complement(7212..7502)
                     /locus_tag="PLP58_17890"
     CDS             complement(7212..7502)
                     /locus_tag="PLP58_17890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ10457.1"
                     /translation="MASRKHSGSNLCFLTAWILFLLSIGLVRLGSVVVEMLDANANIV
                     LDELGQPVLVEPFWANMLYHWEANLLLLLSALMLLAGIFLRVRGGGTLRSQN"
     gene            7587..8018
                     /locus_tag="PLP58_17895"
     CDS             7587..8018
                     /locus_tag="PLP58_17895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ10458.1"
                     /translation="MKPVDSSDRNDSGAGAGWRAAWLVILVLASVMPCLHWWYDIAGI
                     IERFRNPPGLLPAHWLFFRWTGEMAGCALVFAAFAGFWAWRHPRHRRAAIVIAVIAVL
                     ASSVASAMIASWLLTSLIHEEGRHSAEERHNLNTAPAATRN"
     gene            complement(8099..8344)
                     /locus_tag="PLP58_17900"
     CDS             complement(8099..8344)
                     /locus_tag="PLP58_17900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ10459.1"
                     /translation="MASRIESDRHAGTGFAVKDTPPEMNARLFSAMMRKTPQERLLMG
                     FDMMATARKLAWKARARRRKNARSFQRWHGMPLPPGL"
BASE COUNT         1583 a         2857 c         2881 g         1334 t
ORIGIN      
        1 ggcgggcagg accgcgggcc gcgcgggaaa ggcggcgcca aagacaagcc gcagcgcagg
       61 ggcgggaaaa acaggcgcgc cggatcgtag cccgcaggca aagtggcgcg gctttcgccc
      121 cgcttcacag cattgccatc cggctcgcag cgggcatcat aggcgcggcc cgcaccgatg
      181 cgctacctgc tccacgaaca ccccgccttt cccggactcc tctcgaggga ggatctcttc
      241 gtgctggtgg agcgcggctc cctgcgccgg ggggaccttt gcacggacac cctgacgcgc
      301 cgcgaccaca ccgtgggcga agtcatcagc ggcatgaaac cgccgcgcgg cggcacggcg
      361 cgcgtggcgc gcccctcctt ccaggaaatc cgcgccgatc tgccgcacga tccgcccccg
      421 gaggacatgg agcctgacga ggccggggac gaggaaacgg aggccggtga tgaagatgac
      481 gaaggggagg attccgaagg cgacgtggag gatggggatg acggcggcga ggaaaccatc
      541 cagcttcacg cgcatccctc ctggctgaac tatccgggca gctttttcct catcctgctg
      601 ctgctgggcg gcgcgggctg gctgctcgcc tacggcggcg agtacgcgct catccccctg
      661 ctggcggcgc tggcctggct gctgggcgtg gccatcgcgc gcttctccac ggattacatg
      721 gtcaccgacg agcgcgtgga aaaggtctgg ggcatcctcg ggcgcagctc caaggaggtg
      781 cgcatctgcg acatccgcag cattgatgtg tatgaaaaag gcctgctcgg cctgctcggc
      841 atcggcaccg tggatgtctc ctccgccggg aacgcgggca ttgaagtgag cttcaaaaac
      901 atgcgcggcg cccacgcggt gaagcatctc gtgcgcaggc tccagcgcgg ctcgcgcgcg
      961 ggctgagttt ttttcaccgc ctcccaagaa ccccgcagca cccgcgtcat gcacgccgga
     1021 cacgccactc atgcacgacc cgcaccaccg cttccaagag ctgctcacgc gccatgagcg
     1081 tccgctcatc cgctacgccc acagcatcac cgggaacctg gaggacgcgc gggacgtggt
     1141 gcaggacgtg ttcgtcaagc tcagccaaaa cctccacgcc atcggggagg cccgcctcac
     1201 ctcctggctc ttcaccgtct gcaaaaaccg cgcgctcgac cacctgcgca aacaccaacg
     1261 cctcatcgtc atggaaaacg aaaccctcga tctggaaacc tcccctgccc cgcgccccgg
     1321 tgacgagctg gatcagcgcg agaccgcctc caccctgcgc gccctcattg atgagctgcc
     1381 cgcccgccag cgcgaggccg tgcgtctcaa gttcatcgcc ggcctcgatt acaaggagat
     1441 cagccaggcc atgcagacca gcctcggcaa cgtcggctac ctcatccacc acggcgtgca
     1501 ggccctgcgc ctgaagtggc gggagatgga gcagggcgcg ggagtggcgc gggcctagcc
     1561 gcgcttccag tcacaaaaca ttcaacaagc aaacgacatc cccatgaaac ccgaacacat
     1621 caccgcctgg gcgctcgacg agctgccgcc cgccgaaagc gccgctctgg aagacgcgct
     1681 ccaggaaaac ccccaatcgc tcaacgaagc gcgggacacg cgcgccttct gcgacctgct
     1741 cacccgcgag ctgcgcgacg actcgctggc gctcgctccc gatcaacgct cgcaattgga
     1801 atgcctctca gaggacggcg gcatggggca ccctgcctgc gccggacagg cggcacgcct
     1861 gtcccccatg caaaacggcc gggtcacact cgcgcaatcc aagcctcatc atggctggag
     1921 ccgtcatggc atcaccggac tcgcggcggc tgcatgtgtg gcgctgctgg cgtggatggg
     1981 catgaagcag gagatgggga cgcggacatt gggcgaagat ggaaaggaca aggagccgcc
     2041 agtggagcct aacgcagcca ctgtgactgt cacacctggg aagccgcagc ctgaaatcag
     2101 gcagaaggtg aatgagaaaa aacgtgatgg tttggtgctg acctcgcctg ttcgcactga
     2161 tgctgaatcc agctctcttg ttcaggcggc ggattttccg agcttgccgc cggcgcaacc
     2221 cagctttggc aaaacaccgc tctccgtacc gttggagcgt ggagctttcg gaggtggttc
     2281 cccgcccatg ccagccgccg cccccgcagg gcccacaccc ttgttcgctt tcaagaatgg
     2341 cagcggcacc ttgaacgaca ctcgcgacgc ggttctccca cgcacgaata cggctgccgg
     2401 agaaggagaa aaaaaagcgg cggactcctt ctccaatctg aacgcccccg cggatgaagc
     2461 gcgcgctttt cgccgggcgg acaactcgaa cgaatcctac acccgcatca ttgagaacag
     2521 cctgctcgag gtccagcgcg agccgctttc caccttctcg attgatgtgg acacggcctc
     2581 gtatgcgaat gtgcggcgct ttttgaacca gaacacccgc ccgccagcgg acgcggtgcg
     2641 catcgaggaa ctcgtgaatt acttccccgc gcaggaggag gggccgccgc cgggagcgga
     2701 gcatcccttt ggcgtgaagg tggagatttc ctcctgcccc tggcagcccc ggcaccgcct
     2761 ggcccgcgtg catctccagg cgcgggacat cggccgggag cggccggcct cgaacctcgt
     2821 cttcctcgtg gacgtgtccg gctccatgag ttcggacgac aagctgccgc tcgtgcggcg
     2881 cagcctgcaa atgctggcgg agcgtctcgg cgagaatgac cgcgtctcca tggtcaccta
     2941 cgccggcggc acgcaggtgg tgctgccctc ggtgaacggt ctgcaaaaag cggacatcat
     3001 cgccgccata gaccgccttc agtccggcgg cagcacgcat ggcagcgcgg gcatccgcct
     3061 ggcgtatgag caggccgtgg cgggtttcat ccagggcggc gtgaaccgcg tcatcctctg
     3121 cacggacggc gacttcaacg tgggcatcag cgaccccgcc gaactggagg gcttcatcac
     3181 cgaaaaggcg cgcagcggcg tcttcctcag cgtgctcggc tttggcaccg gcaacctgca
     3241 ggaccgcacc atggaaaccc tggccgacaa gggcaacggc aactacgcct atgtggacag
     3301 cctcggcgag gcgcgcaaag tgctcgtcga gcagatgaac ggcaccctcg tcaccgtggc
     3361 caaggatgtg aaaatccaga tcgagttcaa ccccgccgtg gtgcgccaat accggctcat
     3421 cggttatgaa aaccgcctgc tcgccaaaga ggacttcaac gacgacacca aggacgccgg
     3481 cgagatcggc gccgggcaca atgtcaccgc cctttatgaa ctcgtccccg ccagcctgcc
     3541 gcccggcgag cctgcgcgcc cgctggtgga tgccctcaga taccagcgcc agatcgccag
     3601 cccgccggcg ctggccgcca cacccgccgc cgccagcggc gaggccataa ccgtgaagct
     3661 gcgctacaaa gagcccgatg gcgggaccag ccgcctgctc gaagtgccgg tgcgggacac
     3721 gggcgcggac ctccgggcgg cgggaggcga gctccagttc agcgcggcgg tggccggctt
     3781 cggcctgctg ctgcgcgggt catcctacgc cggccagctc acctgggagc aggtgcggga
     3841 gctggcgctc aaaggcaagg gcgccgatcc cctcggttat cgcggcgagt tcatccagct
     3901 cattgaaaaa gcgcgcggtc tcagcgggca ggaccgcgcg ccgccctgag gctcactcct
     3961 cgtccgccag caccagcgcg cgggagaagt cgcacaccgt cagccgcgcg cgcgccttcg
     4021 cgcccggctc caggatgagc ggcgcggact cctccgggcg gaaggccagc acggcgcgtc
     4081 cgttcggcag gcgggcgtgc caggtgcgcg cgctttgcgc gccctcgatg gtcagcagcg
     4141 tgtcaacggt ctgcgggatc atggcacatc agcggcgggc ggcgctttct tcgccgccgg
     4201 gccggcgggt tcgggcctgg cggcgggcca tttcaccttg ctggcctcca ggtgcgcgct
     4261 ggcgcgcagc atgtccaccg cgcgctggag caccgtgtcg cgcagcagcg gcgcgtcctc
     4321 cccgctctcc ccgcccgtgc tgcggcggat gtaggactcg atctcggggt tcttgcgcgc
     4381 gagcagcgcg gcctcgttga agcgcggcac ttgcgtgtca aagatggtgt cccccacggt
     4441 gacgcgggcg tcgaagatcc tgtgcttgtc ggcggcgggc aggcgcaccg ggaagtcggg
     4501 cggcacgccc tttttgaaaa acgaggtgtc gtcggccagc agcatctcgg ccctggcgaa
     4561 gcgcagcagc catgcgtcat ccagcggcag cgattcgtag cgcaccgtgg cgccgcgtgt
     4621 tttctccccc accaccagcg cctgcctgcg cgcgcgcagc accgccgcca gcgtctcgcc
     4681 aagattgcag gtctccgcgt cggcgagcag gatcaccggg cgcgtccaca ccggctcgcg
     4741 cgaggcgagg aagagccgcg cgtcatcccg ccccggctgc ttgagtttga acatcagctc
     4801 cccgcgcggc aggaacagct ccagcatggc ggccgccagc tcgaactccc ccggcggcgc
     4861 cgggctgcgc aggtccagga tcaaatgccg cgtccgcgcg tgcccgcgca gcttttgctc
     4921 catcgccggc gccgtgccct cgtcgaaact gcgcggcttg agatacagga tgtcatccgc
     4981 cagcagctcc gccagcaggc ccgtgcgggt cagcgccggc agcgcctccg tgcgcggcgc
     5041 cagttccgcg cccaggtcca gcctgtccag cagcccctgg aaagccgcgc ggttcagttc
     5101 gtcgtgcgtc aggtccgcgc cccggatgta ctcgccgcgc aggatttgga aggcgttttg
     5161 cagagccccc tgcgacagct cgtccacccc gcgcgcaggc tccgccccct gcgccgccgc
     5221 ctggcaaaac actgccgcca gccaaagccc cgccgccttc cctgccaggg aaaacacggg
     5281 cggcgcggca ttggggaaag acagcatggg cggaggcgtg gactgccgcg catcttctgt
     5341 gctccacccc gcgcgtcaaa ggcgaagcga caaaaaagaa gattcaccgc gccatcccgg
     5401 gggatcccat tcaccgccgc cctttgagcc gggagatcat ttcgcggatg tcaccggcgc
     5461 ctttgacggc gacgtagagg gtgacgctgc tgtaccagag cagcgcggcg gcggtgagga
     5521 ggagccagaa ggtggtcatg gggctttggg ggcgggtttg agcagggagc cggcgatcat
     5581 gcccaatgcg gcggcggcga aggcggcgag gccggcgatg tccttgccga cggaggcggc
     5641 ccaggcggcg gtggagggca gcttttccag ggtgaggtgc gccacgggca cgagcgcgcc
     5701 aagcaggatg ccgccgagcg cgccccagtt gttggcgcgc ttccagtagc agcaggcgat
     5761 gagcagcacg gacatgctgc tcagataaat ggagccggtg agcaggaggt aggaccagac
     5821 attgccctcg atcttgtaaa acaggccgaa gacgagcaga tagacgccga tgagggcgac
     5881 gatgcagcgg ttccaccgga tggcgcgcgc gtcgggccag ggacggcggc ggaagggggc
     5941 caggatgtca ttgtaaatga cgctgcccca actgagcatg tagctggagt tggtgctcat
     6001 gtcggcggcg agcatggcgg cgatgagcag gcccatgagt ccgatgggca ggaagccgcc
     6061 gaggaactcg ggcagggcga gcagggaggc atccgccagg cgcgcccggg cctcgtcgga
     6121 aacggcggcg agttcggcgg tgtcgagtcc ggccagcggc tgcccgacgg gggaggcgga
     6181 gattttttcc gcgacagcgg cgggcagggc ggcgcggtcg gtctcgttga gagccttcag
     6241 cggcgcccag ccgagcacgg ccagtgcggc catgccccag atgccgggca ggaggaagcg
     6301 gcagacgaag aagaaactgg tgcgcgtgta gatgcgccgg ctggtggcgc tgtcgcgcgc
     6361 ggagagcacg cggacgatga cggcctgcca ggtgagggcg gcggcggtgt tggcgatgaa
     6421 ttggttgatg aggaagggcc agccgaggga tggatttgcc agggggttga acccgccctc
     6481 gccatggtgc gcgcgcacgc tctccaccac gcggtcccag ccgacttcct ttaacaccag
     6541 cacgctgacg acgacgaggc ctgcgctcat gacgatgaat tgcaggtagt cggtgacgag
     6601 cacgctgagc atgccgccga gcacggtgta gatgacgacc atgagcagga gggcggtcat
     6661 gagcagcgcc aggtgggaga ggtcgaagcc ggcgacggtg cagaggaatt tgccgccgat
     6721 ctggaggaag acgcccatgt tcagcaggcc gcccatgacg atgacgacgc cggagagcca
     6781 gcgcacggcg cgcccgtagc ggcgctcgaa catttccggc agggtcatgg ctccggcctc
     6841 gcgcaggggc ttgatgcaga agccggtgag gccgatgagc gccatggccg ccgcctggca
     6901 cagccccggc acggcgccgc tgaagccctg ggtgtagccg gcctgcgcgg tgtacatgca
     6961 ggtgatgatg ccgaactcgg tggccgccag ggaggcgatg ccgagatgca gcctcacctc
     7021 ccgcccggcc acgaggtaat cctccagctt ccccacatgc cgccgcatgg ccagcccggc
     7081 ggccatggtc accagcaggt aaatgccgac gatgccgcca tcgagcagcc agtggaaatg
     7141 ggtgggggcg ggcatgtgtg cgggcgggga atgacttcga aaggtagaat cgccgcctag
     7201 gaagtgtcga atcaattctg gctccgtagg gtgcctccgc cgcgaacgcg gagaaaaatt
     7261 cccgccagca acatgagggc gctcagcaac aggagaagat tcgcctccca gtgataaagc
     7321 atgtttgccc agaaaggctc cacaagcacg ggctgcccta gttcatcaag cacgatattg
     7381 gcatttgcat caagcatctc gaccacaacg ctgccaagac gaaccaaccc gatactgagg
     7441 aggaatagaa tccatgccgt cagaaagcaa agattggacc ccgaatgttt cctggatgcc
     7501 atgaatgaaa cctatcccat gaatggaaat ccatccaaac tttcaatttc aaaacggaga
     7561 cgccggacct tattaatgcc atcaccatga aacctgtgga ttcgtcagac agaaatgaca
     7621 gcggagcggg agccgggtgg cgggcggcat ggctggtcat cctggtgctg gcttctgtca
     7681 tgccctgcct tcactggtgg tacgatattg cggggatcat cgaacgattc cggaatccac
     7741 cgggtttgct gcccgctcac tggctgtttt ttcgctggac gggtgagatg gcaggctgcg
     7801 cccttgtgtt cgcggcattt gcgggctttt gggcgtggag acatccccgg cacaggcgcg
     7861 ccgccatagt gatcgcagtc attgccgtcc tggcttcgag tgttgcgagc gccatgattg
     7921 cgtcatggtt gctgacctcg ttgatccatg aggagggccg gcacagcgcc gaagaacggc
     7981 acaatctgaa cacggcgcct gccgcgacca ggaattgaat caaagatcga agacgcaagg
     8041 catcaacaac ccgatgccgt ccgccggagc atggaagcat gaggggaggc ggatgggatc
     8101 acaaaccagg tggcaggggc attccatgcc agcgctggaa agagcgcgcg tttttccgcc
     8161 tccgtgcccg cgccttccag gctagcttgc gcgcggtagc catcatgtcg aagcccatga
     8221 gcaggcgctc ctgcggggtc ttgcgcatca tcgccgagaa cagccgcgcg ttcatctccg
     8281 gcggcgtgtc cttcacggcg aaacccgtgc cggcatgtct atcagattca atgcggctgg
     8341 ccatgttttg agatgctccg tgtcgcatcc gctggcaatc aggtcctcca cgtcggcgaa
     8401 tttcgcgtct ttacatcggc agccggcgca tggtatttgg acgtaaatca acgggatgac
     8461 gggcggcatt gtctgaaatc ccatccgctg tcctgaagcc cccgacccca tgaaacctcc
     8521 ctccccgcgc ccgctggcgg ctgccgtcct ggccttgtgc tgcgcggcgg gcgtgatgct
     8581 gtggctggcg ggccgggatg aggcgcgacg ttttttcccc caagaccagg cggcggacgc
     8641 gcggcggagc gcggc
//
LOCUS       DAOMYJ010000218         8561 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Prosthecobacter sp. isolate AMR_MDS_5629
            NODE_2757_length_8561_cov_23.425817, whole genome shotgun sequence.
ACCESSION   DAOMYJ010000218 DAOMYJ010000000
VERSION     DAOMYJ010000218.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867850
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prosthecobacter sp. (wastewater metagenome)
  ORGANISM  Prosthecobacter sp.
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Verrucomicrobiaceae; Prosthecobacter.
REFERENCE   1  (bases 1 to 8561)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8561)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.30
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:34:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,742
            CDSs (total)                      :: 4,684
            Genes (coding)                    :: 4,676
            CDSs (with protein)               :: 4,676
            Genes (RNA)                       :: 58
            tRNAs                             :: 55
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8561
                     /organism="Prosthecobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2757_length_8561_cov_23.425817"
                     /isolate="AMR_MDS_5629"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1965333"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1451
                     /locus_tag="PLP58_17905"
     CDS             <1..1451
                     /locus_tag="PLP58_17905"
                     /EC_number="1.4.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012434.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="Glu/Leu/Phe/Val dehydrogenase"
                     /protein_id="HRJ10460.1"
                     /translation="LPERRILPMSRAMPRDAFHESVSHYFNRAASYSKVDEGLIDQVR
                     CCNSAYRMRFPVRKDDGKITVVEAFRAEHSHHRQPTKGGIRFSPHVTMDETIALAALM
                     TYKCAIVGVPFGGAKGAVCIDPSTSSEGFRERVTRRYTAELIRKNFIGPDIDVPAPDY
                     GTGEREMGWIADTYKNMRMNSGDAYACVTGKPLSMHGIPGRRQATGLGVFYGIRACME
                     NAQDMRALGLAPGVAGKRVIVQGLGNVGYHAAWYLQKEGGALITGIIEREGGIFNADG
                     LDVEEVFRHRQAGRTLLHYPGAENVEGGMALLERECEILVPAALENQITAENAPRVQA
                     KIIAEAANGPVDFEAESILRQRGVLMLPDILMNAGGVTVSYFEWLKNRAGVSFDRMIS
                     RHEELVKRELIAAIEGMTGLDMPVEKLPRLVHGPSEEELVVAALEQSMVRAYQRVWKC
                     WKERDLPDLRTAAFLLGLELVAESYRHHGIFP"
     gene            1515..2342
                     /locus_tag="PLP58_17910"
     CDS             1515..2342
                     /locus_tag="PLP58_17910"
                     /inference="COORDINATES: protein motif:HMM:NF024242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HRJ10461.1"
                     /translation="MSAKTDIFSLVPPGSRHRVVGERVTVLPGCTVDVLTARKIDLQQ
                     WELHRLSDPYWRCYWPVGAGGEVEINGRRTELAPGFLYLIPPHTAFSGRNSRPFSKWY
                     VHFNLGAAGDRAAPGIHVLKATPAAKAALRELMRLDPGESVFSWPAVRLVAEAVARLP
                     ASVWAGQRLDPRLRGALDFMSAHLALKLTAEQIARFAGLSVRNLNHLFQQELKLPPMR
                     VLLDYRLDHACGLLRHGDASIDEIAEQSGLVNRHYLSRMMRQYRGTSPAAYRRENAG"
     gene            2452..3777
                     /locus_tag="PLP58_17915"
     CDS             2452..3777
                     /locus_tag="PLP58_17915"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024968.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HRJ10462.1"
                     /translation="MRILHLTPGTGTFHCGSCLRDTALIKGLRVRGHDAMMAPLYLPL
                     VNDREAASPELPVRVGGISLYLQQKLPWTRHLPRAFHRWLNKPERLRSAARLMGMTRA
                     KDLGEMALGALVGEKGPQWPEWRRLLDWIKTEAKPDVISLSNSLLIGLCPAIERDLGI
                     PVVVSLQGEDSFLDTLIDPYREQAWEAMRVNARHVTRFIAPSKYYAGVMSARLDAPAE
                     KMAVVYNGIDANSFGTADPDPNWPVVGYFARMIHGKGLTTLVDAFIELSKRNRVPRLK
                     LKIGGAKTPADEKYVAGLERKLAEAGCEQKVEWHPNLSFADKLKFFRNLTVLSVPATY
                     GEAFGLYLLEAMASGVPVVQPDHGAFPEIIGMTGCGVLCRPDDPSALADALENLLLDD
                     HAREQMTNRGIQGVRSEFSVARMAERFEEVLMAARERTVPAGVHEHSGA"
     gene            complement(3793..5019)
                     /locus_tag="PLP58_17920"
     CDS             complement(3793..5019)
                     /locus_tag="PLP58_17920"
                     /inference="COORDINATES: protein motif:HMM:NF012694.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HRJ10463.1"
                     /translation="MLKKVTLTNVNTGHKSLTIVDTDTHEKALIGCGVAPNETASIED
                     VTGADEKIQRFTSPKGGVEDCGAFFSGLGRCLERNISMAKSLKLQVNRVRSARYKGMI
                     AEVIHSISMGEKFSDAMRKFPDLFSEDILSLIIAGEEAGQLAKVCRRIGFSKKKSSKT
                     LKKLKGALIYPGVVIVLGVAVVIVMSFTLVPAMSKLFTSFGTELPTPTKVLIWLSNLF
                     IHKPYMVGVPVLALYFFFANFGRLMAQRWTQDLSLRIPVVGPLIRKSAAAAGFRTMSM
                     LTESNVRLTSALEITASATWHYHYKEFFLRLRDHIVVGRTLHEAFLMESHWLGSDGRN
                     LCGLIELASETGSGTEMLAEIADDYEEELENMAASLDKLIEPLTMLILGVLVGFLIYA
                     IYGPMFSLGDVILKKK"
     gene            complement(5034..5513)
                     /locus_tag="PLP58_17925"
     CDS             complement(5034..5513)
                     /locus_tag="PLP58_17925"
                     /inference="COORDINATES: protein motif:HMM:TIGR02532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HRJ10464.1"
                     /translation="MKNRPHHRASRAGYTLIEVLAAAAVTAIGMSAAISLTSALMLQE
                     ELAHRVAITRNYQENMARVWQLGLSAFGTGGQASVASVMPTVNDSPMLSDAVTGVPTI
                     IETGTVNPSGLGAMQAAVVTASVNVSQNPRVKIQGNSLTLNVYRPKALTDLRASSSR"
     gene            complement(5510..7423)
                     /locus_tag="PLP58_17930"
     CDS             complement(5510..7423)
                     /locus_tag="PLP58_17930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ10465.1"
                     /translation="MKSAFQHHIHQRGALMGATLMLLLVGGLFLSAWLSLMSTRAVQV
                     SWLENAVQRRLSLENSRLLAWQSAMEHGFLPAHDLGSLTSGILGASTGGILPVKGWDE
                     VDALASMLAPGAMTSVFPYNNTGLRPGPAFLAHATQIRPSALSSGLDNFTSHLFLKSR
                     PPVLAGDLFIVYRKPDSAVTEIDVHKTSGAHHAYWQVMGRAVIRHPASLFARTTSRVD
                     LPFRARSLYIQTHDPFNNTPVSGIALNGGTLLPSNLPTSPGTTGASQPNLLSSTTSTV
                     TNTVTNTTGSLVGGQRLAGTGGKLIGGVTSGVTGTVGVVTGTVGGVTDVVGGVTDTVG
                     GVTDVVGGVTDTIGGVVDTTLGLTEQALALLRASLTVETGRVFDGHLNVVRNDLHPSN
                     SLHHIINREVTAGRAGVDEVDVYVKSSDKTLPWWMEEYTGLGGDRPPHPPPGYPSGYP
                     TQFKTLYVRLDHPGLRHLRIRNVIHQIVFVGQSGASAFEAAGALPPVIITLIQDAGQP
                     VGNICFENENNRRLILGCRHPHGQKLDLSWRGAPVSGSDLRWRLVFINEHQSVWLALH
                     DNPVINVRWIGGVMTNWFFKRYASTGPRADRLTFIADDAAPTLPHTGPSYASLLPRDL
                     WLEHYFLPTPPSS"
     gene            complement(7433..8422)
                     /locus_tag="PLP58_17935"
     CDS             complement(7433..8422)
                     /locus_tag="PLP58_17935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ10466.1"
                     /translation="MKHARTGKFRGGFTSLELVLVMFLSAVLIGAVVISYGALVRAQP
                     KVSSMASVPLGSARVQHYYGASGTSRNVPVAPHYGMLALAEELREQFLHDVISATAVF
                     CLPRESHNTWRPSRIPWSSLEHEELDTPQKFRAHVIAAAGVPASLYRDYRNPLGTSET
                     TPSPNATIFILGFSKSEGHLSVSSIYDVDVVRFTGAAEPQGFHASVKRYAPKPAALDD
                     EPLVYSSGYEVFFPPSNPVARSLADWSSDDFTPLFVTFERSSRLSVREGAAIDRFKTA
                     AERPFYFIWWPDPAMRHLGMQTNTAAPATPQHAYNHMAGRTAFMFTVPMFPAL"
BASE COUNT         1538 a         2398 c         3100 g         1525 t
ORIGIN      
        1 gcttgccgga gcggaggatt ctgccaatgt cccgcgccat gccacgcgat gcatttcacg
       61 aaagtgtctc ccattatttc aaccgggcgg cttcctactc gaaggtggac gaggggctca
      121 ttgaccaggt gcgctgctgc aactcggcct acaggatgcg cttcccggtg aggaaggacg
      181 acggcaaaat caccgtggtg gaggccttcc gcgccgagca cagccaccac cggcagccga
      241 cgaagggggg catccgcttc agtcctcatg tgacgatgga tgagacgatc gccctggcgg
      301 cgctgatgac ctacaaatgc gccatcgtgg gcgtgccctt cggcggcgcg aagggggctg
      361 tgtgcataga cccctccacc tcgtcggagg gtttccgcga gcgtgtcacg cggcgctaca
      421 cggcggagtt gatccgcaaa aatttcatcg gccctgacat tgacgtgcct gcgccggact
      481 acggcaccgg cgagcgggag atgggctgga tcgccgacac ctacaaaaac atgcgcatga
      541 acagcggcga cgcctatgcc tgtgtcacgg gcaagccgct gagcatgcac ggcattcccg
      601 gacgcaggca ggcgacgggg ctgggcgtgt tttacggcat ccgcgcgtgc atggaaaacg
      661 cgcaggacat gcgggcgctg ggactggctc cgggtgtggc gggcaagcgc gtcatcgtgc
      721 aggggctggg caatgtgggc taccatgccg cgtggtatct gcaaaaggag ggcggcgcgt
      781 tgatcacagg catcattgag agggagggcg gcattttcaa cgcggacggt ctcgatgtgg
      841 aggaggtctt ccgccaccgc caggccgggc gcactctgct gcactatccc ggcgcggaga
      901 atgtggaggg cggcatggcg ctgctggagc gcgagtgcga aatcctggtg ccagcggcgc
      961 tggagaacca gatcactgcg gagaacgctc cgcgcgttca ggcgaagatc atcgccgagg
     1021 cggcgaatgg gcctgtggat ttcgaggcgg agagcatcct gcgccagcgc ggcgtgctga
     1081 tgctgcctga cattctgatg aacgctggcg gtgtcaccgt ctcctacttc gagtggttga
     1141 aaaaccgcgc cggggtcagt tttgaccgca tgatctcccg ccacgaggag ctggtgaagc
     1201 gcgagctgat cgccgccatc gaggggatga cggggctgga catgccggtg gagaagctgc
     1261 cgcgcctggt gcatggcccc agcgaggagg agctggtggt cgccgcactg gagcagagca
     1321 tggtgcgcgc ttatcagcgt gtgtggaagt gctggaagga gcgcgacctg ccggacctgc
     1381 gcaccgccgc tttcctgctg ggcttggaac tggtggcgga gagctaccgg caccacggca
     1441 tcttcccctg agaaagccgc gcttgccact gctggcggtt ccgtcagttt gtgccatatc
     1501 cagcaatccg ccgaatgagc gccaagacgg acatcttcag cctcgtgccg cccggctcgc
     1561 gccaccgcgt ggtgggcgag cgcgtgacgg tgctgccagg ctgcacggtg gatgtgctca
     1621 cagccagaaa aatcgacctt cagcaatggg agctgcaccg gctgagcgac ccgtactggc
     1681 gctgctactg gccggtgggg gcgggcggtg aggtggagat caatggcagg cgcacggagc
     1741 tggcaccggg ctttttgtat ctgatcccgc cgcacacggc cttcagcggg cgcaacagcc
     1801 gcccgttttc aaagtggtat gtgcatttca atctcggcgc cgccggagac cgcgccgctc
     1861 ccggcatcca tgtcctgaaa gccaccccgg cggcgaaagc ggcgctgcgc gagctgatgc
     1921 ggctcgaccc gggggaaagc gttttttcct ggcccgccgt gcgcctcgtg gccgaggcgg
     1981 tggcgcggct tccagcctcg gtgtgggcgg ggcagcgcct ggacccgcgc ctgcgcggcg
     2041 cgctggactt catgagcgct catctggcct tgaagctgac ggcggagcag atcgccagat
     2101 tcgcgggcct cagcgtgcgc aatctcaacc atctcttcca gcaggagttg aagctgccgc
     2161 ccatgcgtgt gctgctggac taccggctcg accacgcctg cggtctcctc cgccacgggg
     2221 atgccagcat tgacgagatc gccgagcaga gcggcctggt gaaccggcat tatctcagcc
     2281 ggatgatgcg gcagtaccgg ggcacatccc ctgcggccta ccgcagggag aacgcggggt
     2341 gagcgctcac ggcgccgtcc gggcaggcgt cacatacgtt ccttttccgg cggagcttgc
     2401 cctctggaaa tggctgcgct aacgatcctg ttcccccacc cgccaacctt catgcgcatt
     2461 cttcatctca ccccgggcac cggcactttt cactgcggaa gctgcctgcg ggacaccgcc
     2521 ctgatcaaag gcctgcgcgt gcgcggccac gatgcgatga tggctccgct ctacctgccc
     2581 ctggtcaatg accgcgaggc cgcgagtccg gagctgccgg tgcgggtggg cggcatctcg
     2641 ctctacctcc agcagaagct gccctggacg cggcatctgc cgcgcgcctt ccaccgctgg
     2701 ctcaacaagc ccgagcggct gcgctccgcc gcgcgcctca tgggcatgac gcgcgcgaaa
     2761 gacctcggcg aaatggcgct gggggcgctc gtcggcgaga aggggccgca atggcccgaa
     2821 tggcgccgtc ttttggactg gatcaaaacg gaggcgaagc cggatgtgat ctcgctgtcc
     2881 aacagcctgc tcatcggcct gtgcccggcc attgaaaggg acctcggcat tcctgtcgtg
     2941 gtttcgcttc agggggagga ctcctttctc gacacactga ttgaccccta ccgggagcag
     3001 gcatgggaag ccatgcgcgt gaacgcgcgg catgtgacgc gtttcatcgc tcccagcaaa
     3061 tactacgccg gtgtcatgag cgcgcggctg gatgctccgg cggagaaaat ggcggtggtt
     3121 tacaacggca ttgacgcgaa ctccttcggc acggcggacc ctgatccgaa ctggcctgtg
     3181 gtgggctact tcgcgcgcat gattcacggc aaggggctca ccaccctggt ggacgccttc
     3241 atcgagctgt caaaacgcaa ccgcgtgccc cggctcaagc tcaagatcgg cggcgcgaag
     3301 actcccgccg atgagaagta tgttgccggc ctggagcgga agctggccga ggcgggctgc
     3361 gagcaaaagg tggagtggca tccgaatctg agcttcgcgg acaagctgaa atttttccgc
     3421 aatctcacgg tgctttccgt gccggccact tacggggagg cgttcggcct gtatctgctg
     3481 gaggcgatgg cgagcggtgt gccggtggtg cagccggatc atggcgcctt ccccgagatc
     3541 atcggcatga cgggttgcgg cgtgctgtgc aggccggacg atccgtccgc gctggccgac
     3601 gctctggaga atctgctgct cgacgatcat gcgcgagagc agatgacaaa ccgcggaatt
     3661 caaggtgtgc gcagcgagtt cagcgtggcc cggatggccg agcgttttga agaggtgctc
     3721 atggcggcgc gggaacggac tgtcccggca ggagttcatg agcattccgg ggcctgaacc
     3781 ccggcgcttt gatcacttct tcttcaagat cacgtcgccg aggctgaaca tggggccgta
     3841 gatggcgtag atgaggaagc caacgaggac gccgaggatg agcatggtga ggggttcgat
     3901 gagtttgtcg agggaggcgg ccatgttttc cagctcctcc tcatagtcgt cggcgatctc
     3961 ggcaagcatc tcggtgccgg agccggtctc ggacgccagc tcaatgaggc cgcagaggtt
     4021 gcgcccgtcg gagccgagcc agtgcgactc catgaggaag gcctcgtgca gggtgcggcc
     4081 gacgacaatg tggtcgcgca ggcgcagaaa gaactccttg taatggtagt gccaggtggc
     4141 gctggcggtg atctcaaggg cgctggtcag gcgcacgttg gactccgtga gcatggacat
     4201 ggtgcggaag ccggcggcgg cggcggattt gcggatgagc gggccgacga cggggatgcg
     4261 cagtgacaaa tcctgggtcc agcgctgggc catgagcctg ccgaagttgg caaaaaagaa
     4321 gtacagagcc agcacgggca cgccgaccat gtagggcttg tggatgaaga ggttggagag
     4381 ccagatgagc accttggtcg gagtcggcag ctcggtgccg aagctggtga agagcttcga
     4441 catggcgggc acgagggtga agctcatcac aatgacgacg gcgacgccga gcacgatgac
     4501 cactccagga tagatgagag cgcccttgag ctttttgagg gttttggagg acttcttttt
     4561 ggagaagccg atgcggcggc agactttggc gagctggccg gcctcctcgc cggcgatgat
     4621 gagggagagg atgtcctcgg agaaaaggtc cgggaacttg cgcatggcgt cgctgaactt
     4681 ctcgcccatg gaaatgctgt ggatgacctc ggcgatcatg cccttgtagc gggcggagcg
     4741 gacgcggttg acctggagtt tgaggctttt ggccatgctg atgttccgct ccaggcagcg
     4801 tccgaggccg gagaaaaacg cgccgcagtc ctcgactccg cctttgggcg aggtgaagcg
     4861 ctgaatcttc tcatcggcgc cggtcacgtc ctcgatggag gcggtctcat tgggcgcgac
     4921 accgcagccg atgagcgcct tctcatgcgt gtcggtgtca acgatcgtca ggcttttgtg
     4981 accggtgttg acgttggtga gggtgacttt tttgagcatg aggagagggg atgtcagcgt
     5041 gacgaggaag cgcgcaggtc ggtcagtgcc ttggggcgat agacattgag ggtcagactg
     5101 ttgccctgga ttttaaccct gggattttgc gagacattga ccgaggcggt gacgacggcc
     5161 gcctgcatgg cgccaaggcc ggagggattg actgtgccgg tctcgatgat ggtcgggaca
     5221 ccggtgacgg cgtccgagag catggggctg tcattcacgg tgggcatgac ggaggcgacg
     5281 ctggcctggc cgccggtgcc gaaggcgctg agtccgagct gccagacgcg cgccatgttt
     5341 tcctggtagt tccgggtgat ggcgacgcgg tgggccagct cctcctggag catgagagct
     5401 gaggtgagac tgatggcggc gctcatgcca atggcggtga cggccgcggc ggcgagcact
     5461 tcgatgagag tgtagcccgc gcggctggcg cgatgatgag ggcggttttt catgacgagg
     5521 gcggggtggg caggaagtag tgctccagcc agagatcgcg cggcagcagg ctggcataag
     5581 aggggccggt gtggggcagg gtgggcgcgg catcgtcagc aataaaggtg agacggtcgg
     5641 cgcgcgggcc ggtgctggcg tagcgtttga agaaccagtt ggtcatgacg ccgccgatcc
     5701 agcggacgtt gatgacggga ttgtcgtgaa gggcgagcca gactgactgg tgctcgttga
     5761 tgaaaacgag ccgccagcgc aagtcggaac cgctgacggg cgcgccacgc caggagaggt
     5821 cgagcttttg cccgtgcgga tggcggcagc cgaggatgag ccggcggttg ttctcgtttt
     5881 caaaacagat gtttcccacg ggctggccgg cgtcctggat gagggtgatg atgaccgggg
     5941 gcagggcgcc ggcggcttcg aaggcggagg cgccggactg gccgacgaag acgatctggt
     6001 ggatgacatt gcggatgcgc agatggcgca gaccggggtg gtcgagccgg acatagagtg
     6061 ttttgaactg cgtgggataa ccggaaggat agccgggagg aggatgcggc gggcggtcgc
     6121 cgccgaggcc ggtgtattcc tccatccacc agggcagtgt tttgtccgag gacttgacat
     6181 aaacatccac ctcgtccact ccggcgcgcc cggcggtgac ttcgcggttg atgatgtgat
     6241 gcagggagtt ggaggggtgg aggtcgttgc gcacgacatt gaggtgccca tcgaagacac
     6301 ggccggtttc caccgtgagc gaggcgcgca gcaaggcgag agcctgctcc gtcaagccaa
     6361 gggtggtgtc aacaacaccg ccgatggtgt cggtgactcc gccgacaacg tcagtcacac
     6421 cgccgacggt gtcggtgact ccgccgacaa cgtcagtcac accgccgacc gttccggtca
     6481 ccactcccac tgttccggtg acaccgctgg tgacacctcc tatgagtttg ccgcctgttc
     6541 cggccagtct ttgtccgccg acaagactgc cggtggtgtt tgtgactgtg tttgtcacgg
     6601 tgctggtggt ggatgagagc agattcggct ggctggctcc agtggtgccg ggagaggtgg
     6661 gcagattgga ggggagcagg gtgccgccgt tcaaggcgat tccggagacg ggtgtgttat
     6721 tgaagggatc gtgcgtttgg atgtagagcg agcgcgcgcg gaagggcagg tccacgcggg
     6781 aggtggtgcg cgcgaagagc gaggctggat gccggatgac cgcgcggccc atgacctgcc
     6841 agtaggcgtg gtgcgcgccg ctggttttgt ggacatcaat ttccgtgact gccgagtcgg
     6901 gcttgcgata gacgatgaac aagtctcccg cgagcacggg cggacggctt ttgagaaaaa
     6961 gatgggaggt gaagttgtcg agtccggacg acaaagcgga aggccgtatc tgcgtggcat
     7021 gtgccaggaa ggcgggtccg gggcgcaggc cagtgttgtt gtagggaaag acgctggtca
     7081 tggcgcctgg ggctagcatg gatgccagcg cgtccacttc gtcccagcct ttgacaggca
     7141 ggatgccgcc tgtgctggcg ccgagaatcc cggaggtgag gctgcccagg tcgtgcgcgg
     7201 gaaggaagcc gtgctccatg gcgctctgcc aggcaagcag gcgggagttt tccagggaaa
     7261 gccggcgctg gacggcgttc tcgagccagc tcacctgcac ggcgcgggtg ctcatgagtg
     7321 aaagccatgc gctgagaaaa agaccaccga caagcagcag catgagcgtg gctcccatga
     7381 gggcgccgcg ctggtggatg tggtgctgga aggctgattt catgatgatg agtcaaagag
     7441 cgggaaacat gggcacggtg aacatgaaag cggtgcgccc ggccatgtgg ttgtaggcgt
     7501 gctgcggcgt ggcgggggcg gcggtgttgg tctgcatgcc gaggtggcgc atggcggggt
     7561 cgggccacca gatgaagtaa aaaggccgct cggcggcggt tttgaagcgg tctatggcgg
     7621 cgccttcgcg cacggacagg cggctggacc gctcgaaggt gacaaacaag ggtgtgaagt
     7681 catcactgct ccagtctgcg agagaacgcg cgactggatt ggagggtgga aagaagacct
     7741 cgtaaccgct ggaatagaca agcggctcgt catcaagcgc ggcgggcttg ggcgcgtaac
     7801 gtttgacgct ggcgtgaaag ccctgcggct cggcggcgcc ggtgaagcgg accacgtcca
     7861 catcgtagat ggaggagacg ctgaggtggc cctcgctttt gctgaagccg agaatgaaga
     7921 tggtggcatt tggagagggt gtggtttccg atgtgccgag ggggttgcga tagtcgcggt
     7981 aaagtgaggc gggcaccccc gcggcggcga tgacatgggc gcggaatttc tgcggcgtgt
     8041 ccaactcctc gtgctccaga gaactccagg gaatgcgcga gggacgccag gtgttgtggc
     8101 tttcacgcgg caggcagaag acggcggtgg cgctgatgac atcgtgtaga aactgttccc
     8161 gcaactcctc ggccagggcg agcatgccgt aatgcggggc cacgggcaca ttccggctgg
     8221 tgccggaagc gccgtaatag tgctgcacgc gcgcgctgcc cagggggacg gaggccatgc
     8281 tggacacctt cggctgggcg cgcacgagcg ctccgtaact gatgacgacg gcgccgatca
     8341 agactgcgga aagaaacatc acaagcacca gctccaggga ggtgaagccg ccgcggaatt
     8401 tgccagtgcg cgcgtgtttc atttggcctc gggatgcctg ccaaagagca tcctataaac
     8461 ggagccttcg gagatgtttg agggcggcgg aggtgtcacc ggcggctgtt ccggcgcggg
     8521 agccggccgc gccgcgctgg cggctttttc cggcgccggc g
//
LOCUS       DAOMYJ010000219         8557 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Prosthecobacter sp. isolate AMR_MDS_5629
            NODE_2760_length_8557_cov_18.782875, whole genome shotgun sequence.
ACCESSION   DAOMYJ010000219 DAOMYJ010000000
VERSION     DAOMYJ010000219.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867850
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prosthecobacter sp. (wastewater metagenome)
  ORGANISM  Prosthecobacter sp.
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Verrucomicrobiaceae; Prosthecobacter.
REFERENCE   1  (bases 1 to 8557)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8557)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.30
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:34:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,742
            CDSs (total)                      :: 4,684
            Genes (coding)                    :: 4,676
            CDSs (with protein)               :: 4,676
            Genes (RNA)                       :: 58
            tRNAs                             :: 55
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8557
                     /organism="Prosthecobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2760_length_8557_cov_18.782875"
                     /isolate="AMR_MDS_5629"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1965333"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1424
                     /locus_tag="PLP58_17940"
     CDS             <1..1424
                     /locus_tag="PLP58_17940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ10467.1"
                     /translation="APAPEFPQAPQTRKTKEELFKPVPVTPPLETEAGTHRAQRARLR
                     AGRARPAASWGTLFSLGLGALGLLLLSWIYWHDAPAKSDEDLRVELPADQTPVIHSHV
                     KLRTFLDSVPPVEDLRLRSLPPWDWDTPSLSEHLRAGGTALDNLRDLLEDEDWHPLHS
                     AWCHTDHSAHAAWPHVIFLIQAQSAYLARRGNEMEAFTAALDLAEMSRRVMEMWSWPA
                     YARVAQDLGLGAAQSLAVLLKDTRLDAPTLAAFQEQYTACRPETAIMRQYCAAFYTHQ
                     KKTLIGARSGEPLDTLPGGIQQPRHARVFFKVNETLALFANLCRDARDEISRPAYAAR
                     TDTGLVMDEPRRPLFYHPNSAGAAWFEDRAALLRQMPPRHHLAQCRHGLVLSLFALRR
                     HLAEKKRPPESLEALVPEWLAQKLTDPFTGNAFGYDAARGLLYSAGADLRADGGRPGL
                     PPLADDLEPTVETGIQSARRPPD"
     gene            1457..1876
                     /locus_tag="PLP58_17945"
     CDS             1457..1876
                     /locus_tag="PLP58_17945"
                     /inference="COORDINATES: protein motif:HMM:NF039485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YidB family protein"
                     /protein_id="HRJ10468.1"
                     /translation="MGLFDTLAKQALGGGNPGDILSSLLKESGGLDGLREKFQDAGLG
                     EAFASWVGGGDNHNVQPAQIRQALGDEALQRLAGKLGFNPQMLLPLLSQFLPQVIDKL
                     TPNGSIENSSPAAEQIQQVLATVMKNGLGGLFGRPPG"
     gene            complement(1877..2455)
                     /locus_tag="PLP58_17950"
     CDS             complement(1877..2455)
                     /locus_tag="PLP58_17950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ10469.1"
                     /translation="MNRARIKRLLYPQLTLRQFLWLGLFGLCGALIAGVYGVLHDLVT
                     YSIGPEYFTRFKFAQFFYLDFHQPPRLLAAKIGFLASAWVGFFAAWFMGRMILPRMTP
                     GAAARLCGKGVLIMLAVTFFAGVTAGVLAPEAEDPRFENWRGMTAMFHVQDTAAFVRA
                     GWIHNGSYLGAFLGLLAALAWLRKRRANPRGA"
     gene            complement(2452..3060)
                     /locus_tag="PLP58_17955"
     CDS             complement(2452..3060)
                     /locus_tag="PLP58_17955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ10470.1"
                     /translation="MLRHLLYFLVCTSLTARTLDAGAMVDQALAAIGGRDKVLTLFRI
                     KETFHFGSTPEPEEGKKRSSRESVIEPPKFWWLGKKERADEPAKYDVWAWTLGILTDP
                     GTKTEALPALEDEGCQCLRLRVSGTVEPAMDLWFSADTHLLRRLDWRGDFYRFSEWRE
                     HDGVKYASRTVIHKLASGKPWFFHDVTGVERLAELPEGLSRP"
     gene            3227..4258
                     /locus_tag="PLP58_17960"
     CDS             3227..4258
                     /locus_tag="PLP58_17960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007414705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HRJ10471.1"
                     /translation="MSNTLPKLHNAMWPGLVGKGDGEGQEPPISLERMLDLTAAANVN
                     GQKFEGIDYFLFLPHTDPNASDDEIKKIADQIASRGFSVGSLVAPVWPGTVGDSAMGD
                     DAQQAKFLDAVKMACRIAKVFNSTGIRKYGVIRIDSAEFGIAKWRENPKANTARIIDT
                     FKKAAAIAKDNGERLAAEGEICWAGMHSWKDMLDVLEGVGDKNALGFQADLAHTYLYT
                     LGYNAPEHALLKEGYSEAEFYAAYEKMTDALRPWTIDFHVAQNDGTVHGAGSHDKTGK
                     HCRADDPNGKLDIVKCAGYWLKDAQARGIQHICWDGCMFPNAVLETPGTWNTILDTMI
                     KVRDAHGWS"
     gene            4383..4775
                     /locus_tag="PLP58_17965"
     CDS             4383..4775
                     /locus_tag="PLP58_17965"
                     /inference="COORDINATES: protein motif:HMM:TIGR04138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ10472.1"
                     /translation="MSDLPFFVAVQTAVAKDPRYDPHAYEFVREALHVAAKHFRDGQE
                     DQHVTGGEVLEGVRMHALAEYGPLALHLLHSWGLRSGEDVGNIVYNLIETGYFGKNDG
                     DSIEDFAGGYDFEAAFTQPFMPSSATPA"
     gene            4831..5970
                     /locus_tag="PLP58_17970"
     CDS             4831..5970
                     /locus_tag="PLP58_17970"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HRJ10473.1"
                     /translation="MKALSRRAGMKSLAAFCLAPRLRAQEEALPEERREGIAGIAREV
                     MAEHQIPGLSLAVARHGRLVHKAAFGMADAETGEACAPEHRFRIASISKPVTSVAVHR
                     LFDRGLLAPEDRVFGRGGRLPQFRAPTPAHEAITLHHLLTHTCGGWGNKKNDPMFQNG
                     GMSHAELIEWTLGGIALENPPGKTYDYSNFGYCLLGRVIEAVTGEAYEAHVRAQVLDP
                     CGVTGMILAGNTRAERAEREVVYHGQRGEDPYRPGMNVRRMDSHGGWLAAAEDLARFL
                     THVDGFPAPADLLKNGSLKAMTTPWSEGASYARGWSVNSRPNWWHGGSLPGTSTLAVR
                     TASGLCWAVLANTRYRDRENPVKDTAPALDRMMWRIARSVPEWRA"
     gene            6028..7584
                     /locus_tag="PLP58_17975"
     CDS             6028..7584
                     /locus_tag="PLP58_17975"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase"
                     /protein_id="HRJ10474.1"
                     /translation="MKTLLATLALLLAAALPPAHAAEKRPNFLFIIFDDMSWDSVGAY
                     GNTWIKTPNLDRLAKEGALFRHAFTSNPKCSPCRASILIGRNTWQLEELSVHNSIFPR
                     KWEVYPDLLEAAGYSVGLTGKGWGPGDFKNHGRTRNPAGPSFDEHKNEPPASAIGKLD
                     YAKNFEAFLAQRKKDAPFSFWMGFQEPHRAYELHSGVRLGKKLEDIQVPPYFPDVRAV
                     RSDLADYAVEVEWADGHIGRARAALEAAGELDNTLIIATSDHGMPFPYVKGQIHEDGF
                     RLPFLARWGSTIQAGRVIEDFVNVRDIAPTFMELAGLKPHPQMTGRSLANLLRSEKSG
                     MIEGRDVMLAGKERHDLGRPDNLGYPVRAIRTPEFLYVHNYHPERWPACNPETNYGNC
                     DDSPTKEVIKLLGGHFYNLSFGFRPADELYRLTDDPHGVRNLANDPAFAEKMAELRGR
                     MMQMLKDEGDPRALGNGVIFDTYEYTGANPSKNWNIWLQNQDEQLSAEAERLSQIKKN
                     TPRQKKQPAP"
     gene            8210..>8557
                     /locus_tag="PLP58_17980"
     CDS             8210..>8557
                     /locus_tag="PLP58_17980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ10475.1"
                     /translation="MRTLALFFLSGALACAGVPEITTLTGKTYRGCEIVRVHPDGVSF
                     THATGAAKVLFEDLPAEWRARLGYDPVKAEAWKREQEEKRKLAEEARRKREAELSQAL
                     ALAQQIELARLRGV"
BASE COUNT         1610 a         2976 c         2662 g         1309 t
ORIGIN      
        1 ccgcgcctgc gccggaattt ccgcaggcac cgcagaccag gaagacgaag gaggagcttt
       61 tcaaacccgt gccagtgacg ccacctctgg agacggaggc aggaacacac cgtgcccagc
      121 gcgccagact acgcgccggc cgggcgcgcc cggcagcctc ctggggcacg cttttctccc
      181 tcggcctcgg cgcgctcggc cttttgctgc tgtcatggat ttactggcat gacgccccgg
      241 ccaagagcga cgaggatttg cgcgtggaac tgcccgcaga ccagacgccg gtgattcact
      301 cccatgtgaa gctgcgcacc tttctagact cggtgccgcc cgtggaggat ctgcggctgc
      361 gctccctgcc gccctgggat tgggacacgc cctcgctctc cgagcatttg cgcgccggcg
      421 gcaccgcgct ggacaatctg cgcgatctcc tggaggacga ggactggcat ccgctgcatt
      481 ccgcttggtg ccacacggat cacagcgccc atgccgcctg gccgcatgtc attttcctca
      541 tccaggcgca gtcggcttac ctggcccggc gcggcaatga gatggaggcg ttcaccgccg
      601 cgctcgatct ggcggaaatg tcgcgccgtg tcatggaaat gtggtcctgg cccgcctatg
      661 cccgcgtggc gcaggacctc ggtctcggag ccgcgcagtc gctggccgtg ttgctcaagg
      721 acacccggct cgacgcgcca accctcgcgg cattccagga gcagtacacc gcctgccgcc
      781 ccgagaccgc gatcatgcgg caatattgcg cggcctttta cacgcaccag aaaaagaccc
      841 tgatcggggc ccgcagcggc gagcctctgg acacactgcc gggcggcatc cagcagccgc
      901 gccacgcgcg ggtctttttc aaagtgaacg aaaccctggc cctctttgcc aatctctgcc
      961 gggatgcgcg cgatgaaatc tcccgcccgg cctacgccgc gcgcacggac acaggactcg
     1021 tcatggatga gccgcgccgc ccgctctttt atcatcccaa ttcggcaggc gccgcctggt
     1081 tcgaggaccg cgccgccctg ctgcgccaga tgccgccgcg tcatcatctg gcccagtgca
     1141 ggcatggact ggtgctcagc ctttttgccc tgcgcaggca tcttgcggag aaaaaacggc
     1201 cgcccgaaag tctcgaagcc ctcgtgcccg agtggctcgc gcaaaagctc accgatccct
     1261 tcaccggaaa cgcttttggc tacgatgccg cgcgcgggct tctctactcc gccggcgcgg
     1321 atctgcgggc tgatggcggc cgccccggcc tgccgcccct ggccgatgac ctggagccga
     1381 cggtcgagac cggcatccag tccgcgcgcc gcccgccgga ttgacttgcc ccgcctcccg
     1441 ccgtaaatgc gcgcgcatgg gactcttcga cacactcgcc aaacaagcgc tcggcggcgg
     1501 caacccgggc gacatcctct ccagcctgct caaagaatcc ggcggcctgg atggcttgag
     1561 ggaaaaattt caagacgccg gtctcggcga ggcttttgcc tcctgggtgg gcggcggtga
     1621 caatcacaac gtgcagcccg cgcagatcag gcaggcgctc ggcgacgagg cgctccagag
     1681 gctcgccggc aagctcggct tcaatccgca gatgctgctg cccctgctct cccagttcct
     1741 gccccaggtc atagacaagc tgaccccgaa cggcagcatt gaaaactcca gccccgccgc
     1801 cgagcaaatc cagcaagtgc tcgccaccgt gatgaagaac ggcctcggcg gcttgttcgg
     1861 acgcccgccc ggctgatcac gcgcccctcg gattcgcgcg cctctttcgc agccaggcca
     1921 gcgctgccag caagcccagg aaggctccaa gatagcttcc gttatgaatc cacccggcgc
     1981 ggacaaaggc cgccgtgtcc tggacatgga acatggcggt catgccgcgc cagttttcaa
     2041 aacgcggatc ctccgcctcc ggggccagca cacccgccgt cacaccggca aaaaacgtca
     2101 ccgccagcat gatcagcacg cctttcccgc acaggcgcgc ggccgcgccg ggagtcatgc
     2161 gcgggagtat catcctgccc atgaaccaag cggcgaaaaa acccacccat gccgaggcca
     2221 ggaagccgat cttcgccgcc agcagccttg gcggttgatg aaagtccagg taaaaaaact
     2281 gcgcgaattt gaaacgggtg aagtattcag gtccgatgct gtaggtcacg agatcatgca
     2341 agacgccgta aacgccggcg atcagcgcgc cgcagaggcc gaacaatccc agccacaaaa
     2401 actgccgtag cgtcagttgt ggatagagca ggcgtttgat gcgcgcgcga ttcacgggcg
     2461 ggacaggcct tcgggcagtt cggccagcct ctccacgccg gtgacatcgt gaaaaaacca
     2521 cggctttccg ctcgcgagct tgtggatgac ggtgcggctg gcgtacttca ccccgtcgtg
     2581 ctccctccac tcgctgaagc ggtaaaaatc gccgcgccag tccaggcggc gcagcaggtg
     2641 ggtgtccgcg ctgaaccaca ggtccatggc cggctccacc gtgccgctga cgcgcaggcg
     2701 cagacattgg cagccctcgt cttcgagcgc gggcagggcc tcggtcttgg tgccggggtc
     2761 ggtgaggatg ccgagcgtcc aggcccacac gtcatacttc gcgggctcgt cggcgcgctc
     2821 ctttttgccc agccaccaga acttcggcgg ctcgatcacg gactcgcggc tggagcgctt
     2881 tttgccctcc tccggctcag gtgtcgagcc gaagtgaaag gtctccttga tgcggaaaag
     2941 ggtgagcacc ttgtccctgc cgccgatggc cgccagggcc tggtccacca tggcccccgc
     3001 atcgagcgtc cgggctgtga ggctggtgca aacgagaaag tataaaagat ggcgcaacat
     3061 gtcccaccaa cgtcaaagat ggcccgtttg tcacgcgcca gttgaaagag atgccgctcg
     3121 cgtgattcaa accgaatttc gaaccgcggc ctttttttgc gtgccatgac gggccgggtc
     3181 gttatcatcg gcgcgctttt cagcatcaaa tccaaacaca cccaccatga gcaacacact
     3241 ccccaaactg cacaacgcga tgtggcccgg cctcgtcggc aaaggcgacg gcgaaggcca
     3301 ggagcccccg atcagcctcg agcgcatgct cgacctcacc gccgccgcaa acgtgaacgg
     3361 ccagaagttt gaaggcattg actacttcct cttcctcccg cacaccgacc cgaacgccag
     3421 cgacgacgag atcaagaaga tcgccgacca gatcgccagc cgcggcttct ccgtcggctc
     3481 cctcgtggcc cccgtgtggc ccggcaccgt cggtgacagc gccatgggcg atgacgccca
     3541 gcaggcgaag ttcctcgacg ccgtgaagat ggcctgccgc atcgcgaagg tcttcaacag
     3601 caccggcatc cgcaaatacg gcgtcatccg catcgactcc gccgagttcg gcatcgccaa
     3661 atggcgtgag aacccgaagg cgaacaccgc ccgcatcatc gacaccttca agaaggccgc
     3721 cgccatcgcc aaggacaacg gtgagcgcct cgccgccgaa ggcgagatct gctgggccgg
     3781 catgcactcc tggaaggaca tgctcgacgt gctcgaaggc gtgggtgaca agaacgcgct
     3841 cggcttccag gcggacctcg cccacaccta cctctacacc ctgggctaca acgcccccga
     3901 gcacgccctg ctcaaggaag gctacagcga ggccgagttc tacgccgcct acgagaagat
     3961 gaccgatgcc ctccgcccct ggaccattga tttccacgtc gcgcagaacg acggcaccgt
     4021 gcacggcgcc ggcagccacg acaagaccgg caagcactgc cgtgccgacg atccgaacgg
     4081 caagctcgac atcgtgaagt gcgccggtta ctggctcaag gacgcccagg cccgcggcat
     4141 ccagcacatc tgctgggacg gctgcatgtt cccgaacgcc gtgctcgaaa cccccggcac
     4201 ctggaacacc atcctcgaca ccatgatcaa ggtgcgcgac gcccacggct ggtcctgatc
     4261 catcatcttc aaaaccccgc aggtccgcgc ctgcggggtt ttttcttgcg cgcgcgcgcc
     4321 gcgcatccat cagagtgcgc cgaacgaatc cgtcacacac cgcgccaagt tatccccctt
     4381 tcatgtccga tctgcctttc ttcgtcgccg tccagaccgc cgtggccaag gatccacgct
     4441 acgacccgca cgcctacgag ttcgtgcgcg aggcgctgca tgtggcggcg aagcattttc
     4501 gcgacggcca ggaggaccag catgtcacgg gcggcgaggt gctggagggg gtgcgcatgc
     4561 acgcgctggc ggaatacggc cccctggccc tgcacctgct gcattcctgg ggcctgcgca
     4621 gcggggagga tgtcgggaac atcgtttata acctgatcga gacgggttac ttcggcaaaa
     4681 acgacggtga cagcatcgag gacttcgccg gaggttacga cttcgaagcc gccttcacac
     4741 aacctttcat gccgtcttcc gcgacccctg cctgaacgag ggcttcaaaa aacaggatgt
     4801 catttgcgca aagccggttt ctctggcggc atgaaagctc tctcccgccg cgctggcatg
     4861 aaaagtctcg ccgccttctg cctggcgccg cgcctgcgcg cacaggagga ggccctgccg
     4921 gaggagcggc gggagggcat cgccggcatc gctcgggagg tgatggcgga gcaccagatt
     4981 cccggattgt ccctggccgt ggcgcgccac ggcaggctgg tccacaaggc ggcctttggc
     5041 atggcagacg cggagaccgg ggaggcttgc gcgccggagc accgcttccg cattgccagc
     5101 atttccaagc cggtcacctc cgtggcggtc caccggctgt ttgatcgcgg gctgctggca
     5161 ccggaggacc gtgtgttcgg acgcggcggc aggctgccgc aattccgcgc cccgacaccc
     5221 gcgcatgagg ccatcacgct gcatcacctg ctcacccaca cctgcggcgg ctgggggaac
     5281 aagaaaaacg accccatgtt tcaaaacggc ggcatgagcc acgcggaact catcgaatgg
     5341 acgctgggcg ggatcgcatt ggagaacccg cccggcaaaa cctacgacta ctcgaacttc
     5401 ggctactgcc tgctcgggcg cgtgatcgag gcggtgacgg gcgaggcgta tgaggcgcat
     5461 gtgcgcgccc aagtcctcga cccctgcgga gtcaccggca tgatcctggc gggaaacacg
     5521 cgcgccgagc gcgccgaaag agaggtcgtc tatcacggcc agcgcggcga ggatccctac
     5581 cgtcccggca tgaacgtgcg gcgcatggac tcgcacggcg gctggctggc cgccgcggag
     5641 gacctggcgc gctttctcac ccatgtggac ggctttcccg cacccgccga cctgctgaaa
     5701 aacggcagcc tcaaagccat gaccacgccc tggtcggagg gcgcctccta cgcccgcggg
     5761 tggagcgtga actcccgccc caactggtgg cacggcggca gcctgccggg caccagcacc
     5821 ctggccgtgc gcaccgcctc cggcctctgc tgggccgtgc tggccaacac gcgttaccgc
     5881 gaccgcgaaa accccgtcaa agacaccgcc cccgcgctcg accgcatgat gtggcgcatc
     5941 gcgcgctcgg tgccggagtg gcgcgcctga gccgcgccgg atgcgctgac agagcgcgcg
     6001 cggggcggcg tttgcatggg gcgctttatg aaaacactgc tcgccaccct cgccctgctc
     6061 cttgccgccg cgcttcctcc agcccatgcg gcggagaagc ggccgaactt cctcttcatc
     6121 atcttcgacg acatgagctg ggacagcgtg ggcgcctatg gcaacacctg gatcaagacg
     6181 ccaaacctcg accgcctcgc caaagaaggc gcgctcttcc gccacgcctt cacctcgaac
     6241 ccgaagtgca gcccctgccg tgccagcatc ctcatcgggc ggaacacctg gcagctcgag
     6301 gagctttccg tccacaacag catcttcccg cgcaaatggg aggtgtatcc cgacctcctc
     6361 gaagccgccg gctacagcgt cggcctcacc ggcaaaggct ggggccccgg cgacttcaaa
     6421 aaccacggcc gcacccgcaa ccccgccggc ccctcctttg acgagcacaa aaacgagccg
     6481 cccgcctccg ccatcggcaa gctcgactac gcgaagaact tcgaggcctt cctggcccag
     6541 cggaaaaagg acgccccttt cagcttctgg atgggcttcc aggaaccgca ccgcgcctat
     6601 gagctgcact ccggcgtccg tctgggcaaa aagctggagg acatccaagt gccgccctac
     6661 ttccccgacg tgcgcgccgt gcgcagcgac ctcgccgact acgccgtcga agtcgagtgg
     6721 gcggacggcc acatcggccg cgcccgcgcc gcgctggagg ccgccggcga gttggacaac
     6781 accctcatca tcgccacctc cgaccacggc atgcccttcc cctatgtcaa aggacagatc
     6841 cacgaggacg gcttccgcct gcccttcctc gcccgctggg gcagcaccat ccaagccgga
     6901 cgcgtcatcg aagacttcgt caacgtgcgc gacatcgccc ccaccttcat ggaactcgcg
     6961 ggattaaaac cccacccgca gatgacaggc cgcagccttg cgaacctgct gcgctctgaa
     7021 aaatccggca tgatcgaagg gcgcgatgtc atgctcgccg gcaaagaacg ccacgacctc
     7081 ggccgccccg acaacctcgg ctaccccgtg cgcgccatcc gcacgcccga gttcctctac
     7141 gtccacaact accaccccga acgctggccc gcctgcaacc ccgaaaccaa ctacggcaac
     7201 tgcgacgaca gccccaccaa ggaagtcatc aagctgctcg gcgggcactt ttacaacctc
     7261 agcttcggct tccgccccgc cgacgaactc taccgcctca ccgacgaccc ccacggcgtg
     7321 cgcaacctgg ccaacgaccc cgcctttgcc gaaaaaatgg ccgagctgcg cggccgcatg
     7381 atgcagatgc tcaaagacga gggcgacccg cgcgccctgg gcaacggcgt catcttcgac
     7441 acctacgagt acaccggcgc caacccctcc aagaactgga acatctggct gcaaaaccag
     7501 gacgagcagc tcagcgcaga agccgagcgc ctctcccaga tcaagaaaaa caccccgcgc
     7561 cagaaaaaac agcccgcccc gtgagcgcgc aggcaggaat gcgcggcaca aaagctgtcc
     7621 gcatcacatt cataccgaaa aggggaaaca gaggaagacg cgggtcttcc ccggatgggg
     7681 cgcggcggcg cgtgtagagg aagacgtgag tcttcctgga tgggaggcgc gggcggaatg
     7741 gcgcgtctgc gccgggagcg gggcaaactg acgtttgcct ctacgggcgg cgcggcggcg
     7801 cgtgtagagg aagacgtgag tcttcctgga tgggaggcgc gggcggaatg gcgcgtctgc
     7861 gccgggagcg gggcaaacgg acgtttgcct ccacgggcgg cgcggcagcg catgtagagg
     7921 aagacgtggg tcttcctgga tgggaggcgc gggcggaatg gcgcgtctgc gccgggagcg
     7981 gggcaaacgg acgtttgcct ccacgggcgg cgcggcagcg catgtagagg aagacgtgag
     8041 tcttcccgga ttggaggcgc gggcggaatg gagcgtctgc gccgggagcg gggcaaactg
     8101 acgtttgcct ctacgagggc ggggcaaacg gacgtttgcc tccacgggcg gcgcggcggc
     8161 gcgtgattga acaagcgggg gggatggcgc gtcggaagga gcagtgacca tgagaacgct
     8221 cgctcttttt ttcctctccg gcgcgctggc ctgcgcgggt gtgccggaga tcaccacgct
     8281 gacggggaag acttaccgcg gttgcgagat cgtgcgggtg catccggacg gggtctcctt
     8341 cacccatgcg acgggcgcgg cgaaggtgct gtttgaggac ctgcccgcgg aatggcgcgc
     8401 gcgcctgggc tatgacccgg tgaaggcgga ggcgtggaaa cgggagcagg aggagaagcg
     8461 caaactggcg gaggaggcgc gcaggaagcg tgaggcggag ctgagccagg cgctggcgct
     8521 ggcgcagcag atcgagctgg cgcggctgcg gggcgtg
//
LOCUS       DAOMYJ010000220         8522 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Prosthecobacter sp. isolate AMR_MDS_5629
            NODE_2774_length_8522_cov_19.948624, whole genome shotgun sequence.
ACCESSION   DAOMYJ010000220 DAOMYJ010000000
VERSION     DAOMYJ010000220.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867850
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prosthecobacter sp. (wastewater metagenome)
  ORGANISM  Prosthecobacter sp.
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Verrucomicrobiaceae; Prosthecobacter.
REFERENCE   1  (bases 1 to 8522)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8522)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.30
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:34:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,742
            CDSs (total)                      :: 4,684
            Genes (coding)                    :: 4,676
            CDSs (with protein)               :: 4,676
            Genes (RNA)                       :: 58
            tRNAs                             :: 55
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8522
                     /organism="Prosthecobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2774_length_8522_cov_19.948624"
                     /isolate="AMR_MDS_5629"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1965333"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..704
                     /locus_tag="PLP58_17985"
     CDS             <1..704
                     /locus_tag="PLP58_17985"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HRJ10476.1"
                     /translation="AADVRSAELQLSHCEVRAPISGRVGRALTTPGNYVTDGSTVLTT
                     IVTAAPVHVYVDIDENSLLRLQELRREKKLYTNGQGRVPVELQLSNESGFGHRGHIES
                     FDNRLNAATGSMVVRCEFANEKGELTPGLFARVRLPVTAEYPAMLLDEKAVLTDQANK
                     YVLGVEEKDGQLVSAYKPVVIGPALEGRRIIRSGLKAGEKVIINGMARLPQPGLPVAP
                     VQADAAKQVSSSGAQ"
     gene            741..3929
                     /locus_tag="PLP58_17990"
     CDS             741..3929
                     /locus_tag="PLP58_17990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009958704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0042908 - xenobiotic transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HRJ10477.1"
                     /translation="MNFSSFFITRPIFAGVLSLLIFILGAISLFQLPISEYPEVAPPT
                     VIVTATYPGANPKTISETVASPIEQAINGVENMLYMSSQCTVNGVMTLTVTFKLGTDL
                     DLAQVQVQNRVSQVLPKLPEEVRQFGVTTVKSSPDLTLVVHLLSPNDRYDELYLRNYA
                     TLNVKDELARLPGVGQVQIFGGGEYAMRVWIDPDKAAARGLTSSDIVAAIREQNVQVA
                     SGVIGQQPVKKDVPFELTINAKGRLVSEEEFESIIVKVGPQGEKLRLKDLARIEMGSS
                     EYALRSLINNKRAVGIPIFQLPGANALSLSQSVRAKMEELKQKFPEGVDYAIAYDPTV
                     FVDKSIDAVIHTLLEAVLLVVLVVVVFLQTWRASIIPLAAVPVSLVGTFAVMHMLGFS
                     INNLSLFGLVLAIGIVVDDAIVVVENVERNIRNGLRPLEATRQAMKEVTGPIIATTLV
                     LCAVFIPTAFISGLSGQFYKQFAVTIAISTVISSIVSLTLSPALSTVLLHANDEKKDL
                     VSRLIDFLFGWFFRPFNRFFEWSSNLYVGFVRQLIRFSVIALAVYGALVWATWKGFQI
                     VPTGFVPGQDKQYLVGFAQLPDGASLDRTEDVMRRMSDIALAHDGVRDAIAFPGLSIH
                     GFSISPNSGIVFVGLDDFEERGSPELSGDAIAGALNGKFAAIQDAMVLVLSPPPVNGI
                     GTSDGFKLMIQDRGDQGYAALYQATQALVGAAYGSGRLHQVYSGYTVNVPQLEADVDR
                     EKAKMQGVPLANLFETLQINLGSLYVNDFNRFGRTYQVIAQAEPQFRDDVKDITRLKT
                     RNMSGDMVPIGSLIKVSESFGPDRVTHYNGVLAADLNGAPLPPLSSGQGEAVITELAE
                     KNLPPGFEFQWTDLTYQKIIAGNTAVFVYPLCILLVFMVLAAQYESLRLPLAIVLIVP
                     LCLLFALAGVFLAGGDNNIFTQIGFIVLIGLACKNAILIVEFAKEKFEHGATAFEAAL
                     EACRLRLRPILMTSIAFIAGVYPLVVSTGAGAEMRRAMGTAVFAGMIGVTLLGLFLTP
                     VFYVLVMKLGWKKAARPSATEAPPAEALHSAS"
     gene            3941..5452
                     /locus_tag="PLP58_17995"
     CDS             3941..5452
                     /locus_tag="PLP58_17995"
                     /inference="COORDINATES: protein motif:HMM:TIGR01845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux transporter outer membrane subunit"
                     /protein_id="HRJ10478.1"
                     /translation="MRIFHLLAILAIPAFAQVGPDYERPASAVPPKFKGVTWRAASPA
                     AHLPKGEWWKVFRDSKLNDLMARATAGNQDLKAALARFDQARATARMARADFLPGVSL
                     PLTAERQRTSENQPSPIPLNGLRYDGPAYNAMADFSWELDLWGKIRRQNEGARADAEA
                     AADAVHNVLLGIQAELASSYFQLRALDQEIRLVREAVALRGEALKIAQARVLAGAGSE
                     LEQAQAETEVASAEAEISTLQARRDQYENAIAILTGSSAARFRVTSGGALPAPPDLPA
                     GVPSDLLERRPDISSAERQLAAATARIGVARAQFFPSIRLLGRGGYQSMDLDALLEPA
                     SLMWSYGPSISVPLFAGGKNRFNLQKARAAHDEALAGWRQAFLTAVADVETSLSSIQN
                     LATTSAAQQRARQSAERAASLAKTRYEAGTSPYLDVIEANRTALAMQRATAQTAGQRL
                     AAAVSLIKALGGGWEATAPLPAPAPDPSAQIMPDDESRRGLLSRLKDFIRRRE"
     gene            5520..6686
                     /locus_tag="PLP58_18000"
     CDS             5520..6686
                     /locus_tag="PLP58_18000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009960955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HRJ10479.1"
                     /translation="MKTRAALVHALMTLASLQAADWPQFRGVNGSAISSEAAPGPQLK
                     VAWEKELPGRGLSSPIVIGGKVFLTASSGPDQSNLHVFCFAAADGAVIWERVMKATGR
                     TMAHNKTSVAAPTMCGDSEKVFALFSSNDLFAFDHSGNLIWLRGLTFDYANASNSLGM
                     ASSPVLAGGILVVQSENDSESIALGLDAATGVNRWKKERPKAANWTSAVVWKDTVALQ
                     SSKGLTGINPRTGEIEWDYTDGAATIPSAALTETAIYVPSNGVTALAPEKGAASQLWR
                     AEGLKPGTGSPLVIGDSLFVLNSAGILIKASTRNGDESWKLRLKGPFSGSPVAAGKYV
                     YIGGERGDFQVVDTTAPEGEIVHTVELKDPILSTPAISDGAVYIRSDKRLWQLR"
     gene            complement(6695..7213)
                     /locus_tag="PLP58_18005"
     CDS             complement(6695..7213)
                     /locus_tag="PLP58_18005"
                     /inference="COORDINATES: protein motif:HMM:NF021363.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2062 domain-containing protein"
                     /protein_id="HRJ10480.1"
                     /translation="MISSTLQAFQRQWFRFRRGVKSLALRVFRVKDASERVARGFALG
                     MVINFIPTFGLGVLVSGFVARIFGGNVIAGLAGGASLTFFWPFLFFLNLRMGGLLVRP
                     EIEVDEVADINEKSMNALMWGKSFILGMSVNIILAGIMVYLLVFFLHARLRPAALAWF
                     RRRRLRRSQEAA"
     gene            complement(7289..8047)
                     /locus_tag="PLP58_18010"
     CDS             complement(7289..8047)
                     /locus_tag="PLP58_18010"
                     /inference="COORDINATES: protein motif:HMM:NF009466.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HRJ10481.1"
                     /translation="MISLDLSGKTALITGASQGIGAQIARTFHKAGATVIINHPGLPG
                     TLLDAEALAEELNLARTDSAWIFGADVSDSSQVRAMMGEAQARHGGLDFLINNAAIIR
                     DRTISKMSREEWDDVLSVNLSGVFNCCKHGLEVMREGGAIVSFGSIAAIQGFFGQANY
                     AAAKAGVQAMMRVLSREAARKNIRANSVAPGVIETSMAATIPENVRAEMLKNIPLGRF
                     GAPEEVANVVLFLCSPLASYVTGQTIEINGGWRG"
     gene            8230..>8522
                     /locus_tag="PLP58_18015"
     CDS             8230..>8522
                     /locus_tag="PLP58_18015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ10482.1"
                     /translation="MTWLTFALLTVLSWGVYGVILHKGRGFMPMGAETPHAGLKAFLF
                     VCIAYAVIGAVAALVLKARGSDWSFTTSGISWSLIAGLAGAFGALALVLARAR"
BASE COUNT         1531 a         2919 c         2584 g         1488 t
ORIGIN      
        1 cggcggcgga tgtgcgctcg gcggagctgc agctctccca ctgcgaggtc cgcgcgccca
       61 tcagcggccg cgtgggccgc gcgctgacca cgccgggaaa ttacgtcacc gatggcagca
      121 ccgtgctcac gaccatcgtc accgccgcgc cggtgcatgt gtatgtggac attgatgaaa
      181 acagcctgct gcggctccag gaactgcggc gggaaaagaa actttacacc aacggccagg
      241 gccgcgtgcc ggtggaactg cagctttcca atgagagcgg cttcggtcat cgcggccaca
      301 tcgagtcctt tgacaaccgc ctgaacgcgg ccaccggcag catggtggtg cgctgcgagt
      361 tcgccaatga gaagggcgag ctgacgccgg gactctttgc ccgtgtacgg ctgcctgtga
      421 cggcggagta tccggccatg ctccttgatg aaaaagccgt gctcaccgac caggccaaca
      481 aatacgtgct cggcgtggag gaaaaggacg gccagctcgt gtccgcctac aagccggtcg
      541 tcatcggccc tgcgttggaa ggcaggcgca tcatccgctc cggcctgaag gcgggtgaaa
      601 aggtcatcat caacggcatg gccaggctgc cgcagccggg cttgcccgtc gcgccggttc
      661 aggcggatgc ggcgaagcag gtgtcctcgt ccggcgctca atgacatggg ccaggcccag
      721 gactgcaaca tccactcccg atgaactttt cctccttctt catcacacgt cccatcttcg
      781 cgggcgtgct gtcgctgctc atcttcatcc tgggggccat ctcgctcttt cagctcccca
      841 tctcggagta tccggaagtg gcgccgccga cggtgatcgt cacggccacg tatcccgggg
      901 ccaatccgaa gaccatctcc gagacggtgg cctcgcccat tgagcaggcc atcaacggtg
      961 tcgagaacat gctctacatg tcgtcccagt gcacggtcaa cggcgtcatg acgctgaccg
     1021 tcaccttcaa gctgggcacc gatctcgacc tcgcccaggt gcaggtgcaa aaccgcgtct
     1081 cccaggtgct gccgaagctg ccggaggagg tgcgccagtt cggcgtcacc accgtcaaat
     1141 cctcgccgga cctgacgctc gtcgtgcatt tgctttcgcc caacgaccgc tacgacgagc
     1201 tctatctgcg taactacgcc acgctcaatg tgaaggacga actggcgcgc ctgcccggcg
     1261 tgggccaggt gcagatcttt ggcggcggcg agtatgccat gcgcgtgtgg attgatcccg
     1321 acaaggcggc ggcgcgcgga ctcacctcct ccgacatcgt ggccgccatc cgcgagcaaa
     1381 acgtgcaggt ggcctccggc gtcatcggcc agcagccggt gaaaaaagac gtgcccttcg
     1441 agctgaccat caacgccaaa gggcggctcg tcagcgagga ggagttcgaa agcatcatcg
     1501 tcaaagtcgg cccgcagggt gagaagctgc gcctcaagga cctggcccgc atcgagatgg
     1561 gctccagcga gtacgcgctg cgcagcctca tcaacaacaa gcgcgccgtg ggcatcccca
     1621 tcttccagct gcccggcgcc aacgctctct ctctttccca gtcggtgcgc gcgaagatgg
     1681 aggagctgaa gcaaaagttc cccgaaggcg tggactacgc catcgcctac gaccccaccg
     1741 tcttcgtgga caagtcgatt gacgccgtca tccacacgct gctggaggcc gtgctgctcg
     1801 tggtgctcgt ggtcgtcgtc tttctgcaaa cctggcgcgc ctccatcatc ccgctcgcgg
     1861 cggtgccggt ctcgctcgtg ggcaccttcg ccgtgatgca catgctcggc ttttccatca
     1921 acaacctctc cctcttcggc ctcgtgctcg ccatcggcat cgtggtggat gacgccatcg
     1981 tcgtggtgga aaacgtggag cggaacatcc gcaacggcct gcgtcccctg gaggccacgc
     2041 gccaggccat gaaagaggtc accggcccca tcatcgccac cacgctcgtg ctgtgcgcgg
     2101 tcttcatccc cacggccttc atcagcgggc tcagcggcca gttttacaag cagttcgccg
     2161 tcaccatcgc catctccacg gtcatttcct ccatcgtctc gctcacgctc agccccgctc
     2221 tctccacggt gctgctgcac gcgaatgacg agaaaaagga cctcgtctcc cggttgattg
     2281 actttttgtt tggctggttc ttccggccct tcaaccgctt cttcgagtgg tcttcgaatc
     2341 tctatgtcgg cttcgtccgc cagctcatcc gcttcagtgt catcgcgctg gccgtctatg
     2401 gcgcgctggt ctgggccacc tggaagggat tccaaatcgt gcccaccggc ttcgtgccgg
     2461 ggcaggacaa gcaatacctc gtcggcttcg cgcaactgcc ggatggcgcc tcgctcgacc
     2521 gcacggagga tgtcatgcgg cgcatgtcgg acatcgctct ggctcatgat ggagtgaggg
     2581 acgccatcgc ctttcccggt ttgtccatcc acggcttcag catcagtccg aacagcggca
     2641 tcgtgttcgt cggtctggat gacttcgagg agcgcgggtc gcccgagctc agcggcgacg
     2701 ccattgccgg agccttgaac ggcaagttcg ccgccatcca ggatgccatg gtgctcgtgc
     2761 tctcgccgcc gccggtgaac ggcatcggca cctcggacgg cttcaagctg atgatccagg
     2821 accgcggtga tcaaggctac gccgcgctct accaggccac ccaggcgctc gtcggcgcgg
     2881 cctatggcag cggcaggctg caccaggtgt actccggcta caccgtcaat gtgccgcagc
     2941 ttgaggccga tgtggaccga gagaaagcca agatgcaggg agtgccgctg gcgaatcttt
     3001 tcgagacgct gcagatcaat ctcggcagcc tttatgtgaa cgacttcaac cgcttcggcc
     3061 gcacctacca ggtcatcgcc caggccgagc cgcagtttcg cgacgatgtg aaggacatca
     3121 cccgcctgaa gacgcgcaac atgtccggcg acatggtgcc catcggctcg ctcatcaaag
     3181 tcagcgagag cttcggcccc gaccgcgtca cgcattacaa cggcgtcctc gccgccgacc
     3241 tgaacggcgc gccgctgccg ccgctgagct cgggccaggg cgaggcggtg atcacggaac
     3301 tggcggagaa aaacctgccg ccgggctttg agttccagtg gaccgacctg acctatcaaa
     3361 agatcatcgc tggcaacacc gccgtctttg tctatccgct ctgcatcctg ctcgtcttca
     3421 tggtgctggc cgcgcagtat gagagcctgc ggctgcccct ggccatcgtg ctcatcgtgc
     3481 cgctttgcct gctcttcgcg ctggcgggcg tgttcctcgc cggtggcgac aacaacatct
     3541 tcacccagat cggcttcatc gtgctcatcg gactggcctg caaaaacgcc atcctcatcg
     3601 tcgagttcgc caaggagaag ttcgagcacg gcgccaccgc cttcgaggcc gcgctggagg
     3661 cctgccgcct gcgcctgcgg cccattttga tgacctccat cgccttcatc gcgggggtct
     3721 atcccctcgt cgtctccacc ggcgcgggcg ccgaaatgcg ccgcgccatg ggcaccgccg
     3781 tctttgccgg catgattggc gtcaccctgc tcgggctgtt tttgacgccg gtgttttatg
     3841 tgctggtgat gaagctgggc tggaaaaaag ccgcccgtcc atccgccaca gaagctccgc
     3901 cagccgaagc cctccacagc gcctcctgaa tccatccgcc atgcgaattt ttcatctgct
     3961 tgccattctc gccatcccgg cttttgccca ggtcggcccg gactacgagc gccccgccag
     4021 cgccgtgcca ccgaagttca aaggcgtcac ctggcgcgcc gcgtcacccg ccgcgcattt
     4081 gccgaaagga gaatggtgga aagtctttcg cgactcgaag ctcaacgatc tcatggcccg
     4141 cgccacggcg gggaaccagg acctcaaagc cgcgctcgcc cgctttgacc aggcccgtgc
     4201 caccgcccgc atggctcggg cggacttcct gccgggggtg agcctgcctt tgaccgccga
     4261 gcgccagcgc acctcggaga accagccctc gcccatcccg ctcaacggcc tgcgctatga
     4321 cggccctgct tacaatgcca tggccgactt cagttgggag ctggacttgt gggggaagat
     4381 ccgccgccaa aacgagggcg cgcgtgccga tgccgaagcc gccgcggacg cggtgcataa
     4441 tgtcctgctc ggcatccagg ccgagctggc ctccagctac ttccagctcc gcgcgctcga
     4501 ccaggaaatc cgcctcgtcc gcgaggccgt ggccctgcgc ggcgaggcgc tgaagatcgc
     4561 ccaggcgcgc gtgctggccg gcgcgggcag cgagctggag caggctcagg ccgagaccga
     4621 ggtggccagc gccgaggccg agatctccac cctccaggcg cggcgtgacc agtatgaaaa
     4681 cgccatcgcc attctcaccg gcagctcagc cgcccgtttc cgagtcacat ccggcggcgc
     4741 gctgcccgcc ccgcctgacc tgcccgccgg cgtgccgagt gatttgcttg agcgccggcc
     4801 ggacatctcc agcgccgagc gccagctcgc cgccgccacc gcgcgcatcg gcgtggcgcg
     4861 cgcgcagttc ttccccagca tccgcctgct cgggcgcggc ggctaccaga gcatggacct
     4921 cgacgccctc ctcgagcccg cctccctcat gtggagctac ggccccagca tcagcgtgcc
     4981 gcttttcgcc ggaggcaaaa accgcttcaa cctgcaaaaa gcccgcgccg cgcatgacga
     5041 ggccctggcc ggctggcgcc aggcttttct caccgccgtg gccgacgtgg agaccagtct
     5101 ctcctccatc caaaatctgg cgaccacctc cgctgcccag cagcgcgccc gccagagcgc
     5161 cgagcgcgcc gcctccctgg cgaagacgcg ctacgaagcc ggcaccagcc cctacctcga
     5221 cgtcatcgag gccaaccgca ccgccctcgc catgcagcgc gccaccgccc agaccgccgg
     5281 gcagcgcctc gccgccgcag tctcgctgat caaagccctc ggcggcggct gggaggccac
     5341 cgctccgctg ccagctcctg ctccagatcc gtccgcgcaa atcatgcccg atgatgagtc
     5401 acggcgcggg ctgctcagcc gtctcaagga cttcatcagg aggagggaat aaggccggcg
     5461 catggatcaa agaggttcgc gcgcttgaca gcagggcgcg agacttgaac atggcgcgca
     5521 tgaaaacccg cgccgcccta gtccatgcgt tgatgaccct cgccagcctc caggccgctg
     5581 actggccgca gttccggggc gtgaacggct ccgcgatttc atccgaagcg gctccaggtc
     5641 cgcaactgaa ggtggcatgg gaaaaggagc tgcccgggcg cggtctcagc agcccgattg
     5701 tcatcggcgg aaaagtcttc ctcaccgcgt caagcgggcc ggaccagtcg aacctgcatg
     5761 tgttctgctt tgccgcggca gacggcgcgg tgatctggga gcgtgtgatg aaggccacgg
     5821 gccggacgat ggcccataac aaaaccagcg tggcggcgcc aacaatgtgc ggtgactcgg
     5881 agaaagtctt cgccctcttt tcgtccaacg accttttcgc cttcgaccac tcgggcaacc
     5941 tgatctggct gcgcggtctg acctttgatt atgccaacgc cagcaacagc ctcggcatgg
     6001 cgtcatcccc ggtgctcgcg ggcggcatcc tggtggtgca gagcgagaac gacagcgaat
     6061 ccatcgcgct cggtctcgac gcggccacgg gtgtgaaccg ctggaagaag gaaaggccaa
     6121 aagcggcgaa ctggaccagc gcggtcgtgt ggaaagacac cgtggcgctg caatccagca
     6181 agggcctcac cggcatcaac ccgcgcaccg gcgagatcga atgggactac accgacggcg
     6241 cggccaccat ccccagcgcc gcgctcactg aaacggccat ctatgtgccg tccaatggcg
     6301 tcaccgcgct cgcgccagag aaaggcgccg cgtcgcagct ctggcgggcg gaaggcttga
     6361 aaccgggcac gggcagcccg ctggtcatcg gcgactcgct ctttgtgctc aacagcgcgg
     6421 gcatccttat caaggcctcg accaggaacg gcgatgagtc ctggaagctc cggctcaagg
     6481 gtcccttcag cggatcgccc gtggcagcgg gcaagtatgt ttacatcggc ggcgagcgcg
     6541 gcgacttcca agtggtggac accaccgcgc ccgaggggga gatcgtccac acggtggaat
     6601 tgaaggaccc gatcctgagc acacccgcga tcagcgacgg cgcggtttac atccgcagcg
     6661 acaaaaggct ctggcaactg agatgagaaa aatctcaggc cgcctcctgg gagcggcgca
     6721 ggcggcggcg gcggaaccat gccagcgcgg cgggccgcag gcgcgcgtga agaaaaaaca
     6781 ccagcaggta gaccatgatg ccggccagga tgatgttcac ggacatgccc aggatgaagc
     6841 tttttcccca catcagggcg ttcatgcttt tttcattgat gtcagccacc tcgtccacct
     6901 cgatctcggg ccgcaccagc aggccgccca tgcgcagatt cagaaaaaac aggaagggcc
     6961 agaaaaatgt cagactggcg ccgcccgcca gcccggcgat cacattcccg ccgaatatcc
     7021 gggccacaaa cccggatacc agcacgccca gaccaaaggt gggtatgaaa ttgatcacca
     7081 tccccagcgc gaacccgcgc gccacgcgct cgctggcgtc cttcacacga aagacgcgca
     7141 gggcgaggga tttgacgccg cggcggaaac gaaaccactg acgctggaaa gcctggaggg
     7201 ttgacgagat caatttggca gggggtggcg cgaacatacg cctcgcggag cccggttcaa
     7261 tgacagtgtg caaagctgaa tcatattttc atccgcgcca gccgccgttg atctcgatgg
     7321 tctggcccgt gacataggac gccagcggcg agcacagaaa aagcacgaca ttcgccacct
     7381 cctccggcgc gccaaaccgt ccgagcggga tgtttttcag catttcagcg cgcacgtttt
     7441 ctggaatggt ggcggccatg ctcgtctcaa tcacacccgg cgccaccgaa ttggcgcgga
     7501 tgtttttgcg cgcggcctcg cggctgagca cgcgcatcat ggcctgcacg ccggccttgg
     7561 cggcggcata gttggcctgg ccgaaaaaac cctggatcgc ggcgatgctg ccgaaactca
     7621 cgatggcgcc gccttcgcgc atgacctcca ggccatgctt gcagcagttg aacacgccgg
     7681 agaggttcac cgatagcacg tcatcccatt cctcccggct catttttgaa atcgtgcggt
     7741 cccggatgat ggcggcgttg ttgatgagaa agtccagccc gccatggcgc gcctgcgcct
     7801 cgcccatcat cgcgcgcacc tgggatgagt cggagacatc cgccccgaaa atccacgcgc
     7861 tgtccgtgcg ggccaggttc agctcctccg ccagagcctc cgcgtccaaa agagtgccgg
     7921 gcagaccggg atggttgatg atgaccgtgg cgccggcctt atgaaaggtg cgggcgattt
     7981 gcgctccgat gccctgggag gctccggtga tgagggcggt tttgccgctg aggtcgagtg
     8041 aaatcatggg ctattcaaac gccgcatccc ggcgcgcttg aggaaaaaat cctccggtga
     8101 tctctccgtt gacgccgcct gcctgcggtg acagcatccg ccgcgcgcca cggatgattt
     8161 ttccatgatc caccgcgcca agcatcaacg ttcccctcca acgtgaatcc tccacccgca
     8221 aaaaaacaca tgacctggct taccttcgcg cttctgaccg ttctctcctg gggtgtttat
     8281 ggcgtcatcc tgcacaaggg gcgcggcttc atgcccatgg gagcggagac gccgcacgcg
     8341 gggctgaagg cctttctttt tgtctgcatc gcctacgccg tgatcggcgc cgtggccgcc
     8401 ctggtgctca aagcgcgcgg ctcggactgg agcttcacca cctccggcat ctcatggagc
     8461 ctcattgcag gtctcgcggg cgctttcggc gcgcttgcgc tggtgctggc gcgcgcccgc
     8521 ga
//
LOCUS       DAOMYJ010000221         8474 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Prosthecobacter sp. isolate AMR_MDS_5629
            NODE_2788_length_8474_cov_25.934315, whole genome shotgun sequence.
ACCESSION   DAOMYJ010000221 DAOMYJ010000000
VERSION     DAOMYJ010000221.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867850
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prosthecobacter sp. (wastewater metagenome)
  ORGANISM  Prosthecobacter sp.
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Verrucomicrobiaceae; Prosthecobacter.
REFERENCE   1  (bases 1 to 8474)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8474)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.30
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:34:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,742
            CDSs (total)                      :: 4,684
            Genes (coding)                    :: 4,676
            CDSs (with protein)               :: 4,676
            Genes (RNA)                       :: 58
            tRNAs                             :: 55
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8474
                     /organism="Prosthecobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2788_length_8474_cov_25.934315"
                     /isolate="AMR_MDS_5629"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1965333"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..377)
                     /locus_tag="PLP58_18020"
     CDS             complement(<1..377)
                     /locus_tag="PLP58_18020"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HRJ10483.1"
                     /translation="MRLEAEAVVKRFGAFAALDGASFSTGGDARVVALLGPSGGGKST
                     LLRVLGGLLLPDAGEVRVDGQALPHDEAAALRVLRQNGFVFQGYNLFPHLTALQNVVL
                     PLCEVHGQPRAVAEDRARELLARL"
     gene            complement(367..1122)
                     /locus_tag="PLP58_18025"
     CDS             complement(367..1122)
                     /locus_tag="PLP58_18025"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HRJ10484.1"
                     /translation="MKPATPPVRSLLWALLLLAGVSLVVYALFARVHYTWNWSAVWDY
                     RAQFYWGWLTTLGVSLGAMLLSLVVGFALMLGRRAPAAPVRMLCAGIVELLRGSPLLV
                     QLLVGYYIVATALRVESPLLVGMVLLGFFEGAYLSEIFRGAVESIGASQREAARAVGF
                     DRAQTYRFVIIPQAVRRALPGVTGQLVSLIKDSSLLSVIGIEELVQKVRILNSGSYTA
                     LEGYLPLAAAYLIVTLPLSWYARRLEGRFAYET"
     gene            complement(1139..1807)
                     /locus_tag="PLP58_18030"
     CDS             complement(1139..1807)
                     /locus_tag="PLP58_18030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ10485.1"
                     /translation="MWPLILLLCPATLAAAEVQAHGLLFERWLCVTFFGGHQPGGYTQ
                     KWDIPAEANVAHGGIPVNPKAVKHGAPIGLGDALRQHDIAEPFLLIIGAWEQADAKTK
                     RWVNARAARVEPAQWRALWGGVTRADLERLDAVVKDKALTLEEARARAQAMKTQPPFT
                     TAVIQLNPKIDRSQRRLQCSLRFDDFFTHLAPGAPRGRLERPEIFGVPLPGSFASGAR
                     SFQK"
     gene            1948..7020
                     /locus_tag="PLP58_18035"
     CDS             1948..7020
                     /locus_tag="PLP58_18035"
                     /inference="COORDINATES: protein motif:HMM:NF024589.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HRJ10486.1"
                     /translation="MNAAPPPPARHFPVIRVFVSSTFSDLVAERNALAEKVWPELERY
                     CRQRGFTFQAIDLRWGVPSEAGLDHRTMQICFEELHRAQETSPEPNFLILLGDKYGWR
                     PLPETISKAEYDRLFEHTQSPGEQRTLEDWYRRDANAIPPHHILRARTDSPDGQDYTR
                     VPDKDGRLHDTDAWLAVQETLWAIVNRAYPASLLRERFGEEIDSIPSSVRFQGSATEQ
                     EIWNGALKVENTREHVVAWFREIVTTNGNPEPWQLKRFFDLKPDQSPDLDAADALAAL
                     KTQVEQALAPEPILHAQCRWAKNASGAFTGQVTTDHLAPMCEAILTRLRTLILRQIND
                     YWGTDLSDVDATVAVVRGTKQELDLELRDHRRFAQERGPVDGFVGRDEQVRTLRDYLQ
                     ATSNRPHIVHGPSGSGKTALLAYVAQQPFLPGAESGGVSPLILTRFIGAHPESSTLRG
                     LMTSLCRELRNHFPIPEPLPDTLQKLDEEFYAQLGRATAEQPIYLFLDALDQLEAADQ
                     ARDCFWLRSQINPQVGEAACHARFVASCLSPSEEFPLESEACEPFRRLQTRGLLADEE
                     LGALRETAACELLRRWLADQQRCLTKEQWQSVDAAISASSSSRHPLSLQVLHERLRGW
                     RDFDEPTPLPSSLPLLIQGTLEQLCQPNQHGLLPRIALGYLVSARYGLTEGELLEVLF
                     RDPEMQAQLDAAGQAYGHHLPPEANRYPVAPWSRLRSDLRAYLSERAAPGTTVLSCYH
                     RQVEQAVRMLFLADEAARAERRRLLADYFDDRWDQPDAHALMELPDLLLALPDHQRLY
                     DRLTDFAFPMRKAEIGLLEAIPGDYSHLRKEGPTAFGALLAVWQDFFHEHAHILRRGE
                     ADRSVHKILLQLALEHADDSPVTIAAEQWQSVGYCDWLRLQRVSRVSRLVHLTPNPCL
                     AVLEGHTDSVTHALLLPDGSILSWSAIFSTPDFLSPDRVLRVWDAATGACRIALVGHM
                     RAVSGALSLLDDRILSWSEDETLRLWDCMTGACHAVLEGHKGEVRGALQMLSGDIFSW
                     SEYDWNLRQWDGATGKCHKVLEGHTRAVCGALALPDSRILSWSGDGTLRVWDIVTGAC
                     RAVLQGHTGPVCGALSLRDDRILSWSDDKTLRIWVGETGSCCAVLEGHMALVYGALPL
                     PEGRILSFSKDKTLRVWGTSTGTCDALLEGHAGPVYHALPLRDGRILSWSDDGTLRVW
                     DASTGTCDALLEGHTERVRGALPLPNERILSWSDDSTLRLWDGTTSACVAVLEGHTGL
                     IYGGLPLGDGRILSWSDDKTLRFWDAAAKPHRAVLEGHTRWVNGALSLPDGQVLSWSG
                     DHMLRLWDGISGACRTVLEGHTERVRGALPLPNERILSWSMDNTLRLWDATTGACRAV
                     LEGHTEWVRDAVPLPDDRILSWSWDRTLRVWDPATGACLAVLKGNVGPFRGTLLLPNG
                     RILSWSESDKTLCLWDSVTGTRPTVLKGHTKDIEGALILPDTEILSWSWDGTLRVWDS
                     VTGVCRAVLEGHADGVTGALVLGDGRILSWSRDGTLRIWDIETGACCTVLKGHKLWVK
                     GALSQSHGRILSWSNYDKAHRLWGAVTGACLDVFPDDDLPIRYPDWFHLKRMSEQPES
                     VADDWCADAGGRTARLFHRSGFTSLPAWQAESDCEARLLQPDGTLVVTQANGQVCILK
                     LHHGNRRVTLAEAEEILRAGGK"
     gene            complement(7044..7361)
                     /locus_tag="PLP58_18040"
     CDS             complement(7044..7361)
                     /locus_tag="PLP58_18040"
                     /inference="COORDINATES: protein motif:HMM:NF014505.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HRJ10487.1"
                     /translation="MNSSHLDVVLLPIPFTDLSSHKVRPAIVIGFGTFPGDLFVVPVT
                     SQLANADFPLADWRASGLNVPCGVKGQLCTVEDRLVRKVVGRLTPADSATLQNCLRRW
                     LRL"
     gene            complement(7342..7551)
                     /locus_tag="PLP58_18045"
     CDS             complement(7342..7551)
                     /locus_tag="PLP58_18045"
                     /inference="COORDINATES: protein motif:HMM:NF014059.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF104 domain-containing protein"
                     /protein_id="HRJ10488.1"
                     /translation="MSITMEAIYENGLLRPLSPLSLPEHTRVSVSVDDGKDAERNEWL
                     AESKRRLTGVWDNDRDDVFNELLAP"
     gene            8093..>8474
                     /locus_tag="PLP58_18050"
     CDS             8093..>8474
                     /locus_tag="PLP58_18050"
                     /inference="COORDINATES: protein motif:HMM:NF024931.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium chelatase domain-containing protein"
                     /protein_id="HRJ10489.1"
                     /translation="MVARTYSATLVGVNAVEVEIESHDGGGNPKMFIVGLPDASVKEA
                     RERVSAAIASCGYAMNDGVTTVNLAPADLRKEGPGFDLPIAISLIAHRVRIPIATLAE
                     CAMIGELALNGELRPVRGLLAVALD"
BASE COUNT         1558 a         2801 c         2610 g         1505 t
ORIGIN      
        1 agacgggcca ggagctcgcg cgcgcggtcc tcggccaccg cgcgcggctg gccatgcacc
       61 tcgcacagcg gcagcaccac gttttgcagc gccgtgaggt gcggaaaaag gttgtagcct
      121 tgaaagacga agccgttttg ccgcagcact ctaagcgccg ccgcttcgtc atgcggcaga
      181 gcctggccgt ccacccgcac ctctcccgcg tccggcagca gcaggccgcc gagcactcgc
      241 agcagggtgg acttgccacc gccactcggc cccagcagcg ccaccacgcg cgcatcgccc
      301 ccggtggaaa aagacgcccc gtccagcgcc gcgaacgcgc cgaaccgctt caccaccgcc
      361 tccgcctcaa gtctcatacg cgaacctccc ctccagacgc cgcgcatacc aggacagcgg
      421 cagcgtgacg atcaaatacg ccgccgccag cggcagatag ccctccaagg cggtgtagct
      481 gcccgagttc aggatgcgca ctttttgcac cagctcctca atgccgatga cggagagcag
      541 ggacgagtcc ttgatgagcg agacgagctg ccccgtcaca cccggcagcg cgcgccgcac
      601 cgcttgaggg atgatgacaa aacggtaggt ctgcgccctg tcgaacccca ccgcccgcgc
      661 cgcctcccgc tgcgaggcgc cgatgctctc caccgcgccg cggaagattt ccgagagata
      721 cgcgccctcg aaaaacccca gcagcaccat tcccaccagc agcggagact ccacgcgcag
      781 cgccgtggcc acgatgtaat aacccacaag caactgcacc agcagcggcg agccgcgcag
      841 cagttccaca atacccgcgc acagcatccg caccggcgcc gccggcgcgc gcctccccag
      901 catgagggcg aaccccacga ccaaactcag cagcatcgcc cccagggaga cgcccagcgt
      961 cgtcagccag ccccagtaga actgcgcgcg gtagtcccag accgcgctcc aattccaagt
     1021 gtaatggacc ctggcaaaca gcgcgtaaac caccagcgag acgcccgcca gcagcagcag
     1081 cgcccagagg aggctgcgaa caggaggtgt ggcgggcttc atgcgtgcgg ggcggctttc
     1141 acttctggaa cgaacgcgcg ccggaggcaa acgatccggg caggggcacg ccaaaaatct
     1201 ccggccgctc cagcctcccg cgcggcgcgc ccggcgccag atgcgtgaag aaatcgtcaa
     1261 agcgcaggct gcattgcagg cggcgctggc tgcggtctat tttcggattg agctggatga
     1321 cggccgtggt gaagggcggc tgcgttttca tcgcctgcgc ccgcgcgcgc gcctcttcga
     1381 gagtgagggc cttgtccttg accaccgcgt ccagccgctc caggtccgcc cgcgtgacgc
     1441 cgccccacaa agcgcgccac tgcgccggct ccacccgcgc cgcccgcgcg ttcacccagc
     1501 gcttcgtctt tgcatccgcc tgctcccacg cgccgatgat cagaagaaaa ggctccgcga
     1561 tgtcatgctg ccgcagcgcg tcccccaggc cgatgggcgc gccgtgcttc accgccttcg
     1621 ggttcaccgg gatgcctccg tgcgccacgt tcgcctccgc cgggatgtcc cacttctgcg
     1681 tgtagccgcc cggttgatgc ccgccgaaaa acgtcacgca cagccagcgc tcaaaaagca
     1741 gcccgtgcgc ctgcacctcc gccgccgcca gtgtggcagg gcagagcagc agaatgagcg
     1801 gccacatttt catcctccga gactgccgct ccccggccaa aacgcaagcg caccggcggc
     1861 gcgtgccgcg cggcgcggca aaagcaaaat ccgcaacccc ttccgcaggc ttgactcccc
     1921 cgtgctgtcc cgttgtagca tcatcccatg aacgcagccc caccaccacc ggcgcgccac
     1981 ttccccgtca tccgggtctt tgtcagttcc acgttctccg acctcgtagc cgagcggaac
     2041 gcgctcgctg aaaaagtctg gccggaattg gagcgctatt gccgccagcg cgggttcacc
     2101 ttccaggcga ttgacctgcg ctggggcgtg cccagcgagg cggggctgga tcaccgcacg
     2161 atgcagattt gcttcgaaga actccatcgc gcgcaagaga catccccaga gccgaacttc
     2221 ctgatactgc tgggggacaa gtatggctgg cgaccgctgc cggaaacaat ctctaaagcg
     2281 gaatacgacc gcctctttga gcacacgcag tcgccggggg aacagcgcac actcgaagac
     2341 tggtatcgcc gcgatgccaa tgccatccca ccgcatcaca tcctccgcgc ccgcaccgac
     2401 agccccgacg gccaggacta cacccgtgtg cccgataagg atggccgact gcacgacacc
     2461 gatgcctggc tcgccgtgca ggagacgcta tgggccatcg tcaatcgggc ctatcccgcc
     2521 tcactcttgc gcgagcgatt cggcgaggag atagattcca tcccctcgag tgtgcgcttc
     2581 cagggatccg cgaccgaaca ggaaatctgg aatggcgcgt taaaagtcga aaacacacgc
     2641 gagcatgtcg tggcttggtt tcgggagatt gtcacgacga atggcaatcc agaaccgtgg
     2701 caactcaagc gcttcttcga tctgaagccc gatcagtctc ctgacctcga tgccgctgat
     2761 gcgctcgccg cattgaagac ccaggtggag caagcactcg cgccggagcc aatccttcat
     2821 gcccagtgtc gttgggcgaa gaatgccagc ggtgccttca caggccaggt gaccaccgac
     2881 cacctcgcgc ccatgtgcga ggccatcctc acgcgcctgc gcacgctcat cctccggcag
     2941 atcaatgact actggggcac cgacctctcc gatgtggacg cgaccgtcgc tgtcgtgcgc
     3001 ggcacgaagc aggaactcga cctcgaactc cgcgatcacc gccgctttgc ccaggagcgc
     3061 ggaccggtgg acggcttcgt cggacgcgat gagcaagtcc gcaccctccg cgactacctg
     3121 caagccacct ccaatcgccc gcacatcgtg catggaccct ccggcagcgg caagaccgcg
     3181 ctcctagcct acgtcgccca gcagcccttc ctgcccggtg cggaatccgg tggcgtgtcg
     3241 cctctgattc tgactcgttt catcggcgct catcccgagt cgtccacact gcgcggcttg
     3301 atgaccagcc tctgcaggga actgcggaat catttcccca tcccagagcc gctcccggac
     3361 acgctgcaaa aactggacga ggaattctat gctcaactcg gccgtgcgac cgccgagcag
     3421 ccgatctacc tcttcctcga cgccctcgac cagctcgagg ccgccgatca agcgcgcgac
     3481 tgcttctggc tgcgctccca aatcaatccg caggttggcg aagcagcatg tcatgcacgg
     3541 ttcgtcgctt cctgcttgtc accatcggag gagtttccgc tggagtccga ggcctgtgag
     3601 ccgttccgca ggctccagac gcgtggactg ctggcggacg aggaactggg agcgctccgc
     3661 gaaaccgcag cctgtgaact cctgcggcgc tggctcgcgg atcagcagcg ctgtttgacg
     3721 aaagaacagt ggcagtccgt ggacgcagca atttccgctt cctcgtcatc tcgtcatcca
     3781 ctttccctcc aagtgcttca cgagcgtctt cgcggctggc gcgacttcga tgaaccaacg
     3841 ccgctcccct catcgctccc gttgctcatt cagggaacac tggagcaact ctgccagccg
     3901 aaccagcatg gcctgcttcc ccggatcgcc ctcggctatc tggtcagcgc gcgttacggc
     3961 ctgaccgaag gggaactgtt ggaagtcctc ttccgcgacc cggagatgca ggcccagctt
     4021 gatgccgccg ggcaagctta cggccaccac cttcctccgg aagccaatcg ttaccctgtc
     4081 gccccctggt cacgccttcg atccgacctg cgcgcctacc tgtccgagcg cgccgccccc
     4141 ggcaccacgg tcctgtcctg ctaccaccgg caggttgagc aagccgtgcg catgctgttt
     4201 ctggccgatg aagccgctcg ggcggagcgc cgccgtcttc tcgctgatta ctttgacgat
     4261 cgctgggacc aaccagatgc ccacgccctg atggagctgc cagaccttct gctcgcgctc
     4321 ccggaccacc agcggctcta tgatcggcta accgactttg cctttcccat gcggaaagcc
     4381 gagatcgggt tgctggaggc cattccaggt gactactctc acctacgcaa ggaaggcccg
     4441 actgctttcg gcgctctact tgccgtctgg caggactttt tccatgaaca tgcccacatc
     4501 ttgcggcgcg gagaggcaga ccggtccgtc cacaagattc tgctgcaact cgcgctagaa
     4561 cacgccgacg acagcccggt gacgattgct gcggaacagt ggcaatctgt tgggtattgc
     4621 gactggctgc gcctgcagcg ggtgtcgcgg gtgtcgcgct tggtgcatct gacgccgaac
     4681 ccctgccttg ctgtcctgga ggggcatacg gattcggtca ctcatgcact cttgctaccg
     4741 gacggaagca ttctctcatg gtcagcgatc ttctcaactc cggacttcct atctcccgat
     4801 agggtgctgc gggtatggga cgccgcgacc ggggcttgcc gcatcgcctt ggtcgggcac
     4861 atgcgtgcgg tcagtggcgc attgtcgctg ttggatgacc ggattctctc ttggtcggaa
     4921 gacgagacgt tgcgtctatg ggactgcatg accggagcct gtcatgcagt cttggaggga
     4981 cacaagggtg aggttagggg cgcattgcag atgcttagcg gtgatatttt ttcctggtca
     5041 gaatacgact ggaacctgcg acaatgggat ggagcaaccg ggaaatgcca caaagtcttg
     5101 gaagggcaca cccgtgcggt ctgtggcgca ctggcacttc cagacagccg gattctctct
     5161 tggtcgggcg acgggacgct acgcgtttgg gacatcgtga cgggagcctg ccgagcagtt
     5221 ctgcaggggc acacgggtcc ggtatgtggc gcgttgtcgc tacgggatga ccggattctc
     5281 tcctggtcgg atgacaagac tctgcggatc tgggttggcg agaccggttc ttgctgcgct
     5341 gtcctggagg ggcacatggc tctggtgtat ggcgcgttgc cgctgccgga gggccggata
     5401 ctctcttttt cgaaggacaa gacgctacga gtgtggggca cctcgactgg aacttgcgac
     5461 gccctattgg aagggcacgc ggggccggta tatcatgcat tgccgctccg cgacggtcgg
     5521 attctctcgt ggtcggatga cgggacgcta cgagtgtggg acgcctcgac tggaacttgc
     5581 gacgctctct tggaagggca cacggaaagg gttcggggtg cgctgccgct gccgaacgag
     5641 cggattctct catggtcgga tgacagcact ctgcggctct gggatggaac gactagtgct
     5701 tgcgtagccg tcctggaggg tcacacgggt ttgatatatg gcggattacc cttgggggac
     5761 ggtcggattc tctcgtggtc ggatgacaag acgctgcggt tctgggatgc cgcagccaag
     5821 cctcaccgag ccgttcttga ggggcacaca cgttgggtta atggagcact atcgctgccg
     5881 gacggccaag ttctttcttg gtcgggggac catatgctgc ggctatggga cggcatatcc
     5941 ggagcctgtc gcaccgtcct ggaggggcac acggaaaggg ttcggggtgc gctgccgctg
     6001 ccgaacgagc ggattctctc atggtcgatg gacaacactt tgcggctctg ggacgccacc
     6061 accggggcct gccgcgccgt ccttgagggg cacaccgaat gggttcggga cgcggtgcct
     6121 ctgccggacg accggattct ctcttggtcg tgggacagga cgctgcgggt atgggatccc
     6181 gcaacaggag catgcctcgc cgtcttgaaa gggaatgttg ggccattcag aggcaccctt
     6241 ctgctgccta atggccgcat tctctcttgg tcggaatctg ataagacttt gtgtctgtgg
     6301 gactcagtaa ctggaaccag acccacagtc ctcaagggac acacgaagga tattgaaggc
     6361 gcccttatat tacccgacac ggagattctc tcctggtcgt gggatggaac gctgcgggtg
     6421 tgggatagcg taaccggggt ttgccgcgca gtactggaag ggcacgcgga tggagtcacc
     6481 ggtgcgctgg tcttgggaga cggccgaatt ctttcctggt cacgggacgg gactctacgg
     6541 atttgggaca tagaaaccgg ggcatgctgc accgttctaa aggggcacaa actttgggtc
     6601 aaaggcgcgc tgtcgcagtc acacggccgg attctttctt ggtcaaatta cgacaaggca
     6661 caccggctgt ggggtgcggt gaccggagct tgcctggatg tcttccctga tgacgatctt
     6721 ccaattcgct atccggactg gttccatctc aaacggatgt cggagcaacc ggagagcgtc
     6781 gccgacgact ggtgtgcgga tgctggaggc cgaacggccc gtttgtttca ccgcagcggc
     6841 ttcacatcgc tcccggcatg gcaagcggag tcggactgcg aagcgcgtct cctccagccg
     6901 gacgggacgc tcgtcgtcac gcaggccaac ggccaggtct gcatcctcaa gctccaccac
     6961 ggcaaccgcc gcgtcacgct cgccgaggcg gaggagattc ttcgagcggg tgggaagtga
     7021 gccctctcgg tggacaagac cgctcacaga cgcagccagc ggcggagaca gttctgaaga
     7081 gtggcggagt ctgcgggtgt gaggcgaccg accactttgc gcacgagacg gtcttcaacc
     7141 gtgcagagct ggcctttcac accacagggc acattgagtc cagatgcgcg ccagtcggcc
     7201 agggggaagt ccgcgttggc aagctgagaa gtcaccggca cgacaaacag atcacccgga
     7261 aaggtgccga agccgatcac gatggccggg cggactttgt ggctgctgag atccgtgaaa
     7321 ggaatcggca gcaggacgac atcaaggtgc gaggagttca ttgaagacat cgtccctgtc
     7381 attgtcccac acgcccgtca gacgacgctt ggattcggcc agccactcgt tgcgctcggc
     7441 atctttgcca tcatccaccg aaacgctcac acgggtgtgc tcgggcaagg agagaggaga
     7501 aagcggccgc agcagaccgt tctcgtagat ggcttccatc gtgatgctca tgggcgcgga
     7561 ctatagcgat gcatgaccag tggcgcaagc agactggccg gtgaggatcg tgcgtgtgca
     7621 ggtgccatcc aatcagacgg cccctctcgt cgtcacggag gccaactgcc aagccgccta
     7681 ctcaagctcc accacggcaa ccgccgcgtc acgctcgccg aggcggagga actgcttctc
     7741 tccgctcctg gaggccatgg ccctccgccc atcgccaatc tgcggatcaa cccttcggcc
     7801 acgcagccaa cgcaccctcc cgcagccacg gtttcagcac ctcccacggt agcacatgca
     7861 cgacgaagcc ctgggcgaag gaagccagct cgtagggctc gtgggagaaa ccgatcccgc
     7921 caggggtgag gaaccagttt tcgctcagct cgatccggtc ggtgtcgagc ctcgcttcac
     7981 tcaggggcgc accgggcgcg aatccggcat tccgccgcca ttgggcctca cttgcggcgg
     8041 tcccccttga gcgtccttgc cacgcgcgca tcgcgcttta atctcgcggc gcatggtcgc
     8101 gcgcacctat tcagccacgc ttgtcggggt caacgccgtc gaggtggaga tcgagtccca
     8161 tgatggcggg gggaatccga aaatgttcat cgtcggtctg ccggacgcct cggtgaagga
     8221 ggcgcgggag cgggtgagcg ccgccatcgc ctcctgcggc tatgccatga acgatggtgt
     8281 gacgacggtg aacctggccc cggcggacct gcgcaaggag gggccgggtt ttgacctgcc
     8341 catcgccatc tcgctcatcg cgcaccgggt gaggataccc atcgccacgc tggcagagtg
     8401 cgccatgatc ggcgagctgg cgctcaatgg cgagctgcgg cctgtgcgcg ggctgctggc
     8461 cgtggcgctg gacg
//
LOCUS       DAOMYJ010000222         8437 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Prosthecobacter sp. isolate AMR_MDS_5629
            NODE_2804_length_8437_cov_23.153424, whole genome shotgun sequence.
ACCESSION   DAOMYJ010000222 DAOMYJ010000000
VERSION     DAOMYJ010000222.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867850
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prosthecobacter sp. (wastewater metagenome)
  ORGANISM  Prosthecobacter sp.
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Verrucomicrobiaceae; Prosthecobacter.
REFERENCE   1  (bases 1 to 8437)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8437)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.30
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:34:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,742
            CDSs (total)                      :: 4,684
            Genes (coding)                    :: 4,676
            CDSs (with protein)               :: 4,676
            Genes (RNA)                       :: 58
            tRNAs                             :: 55
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8437
                     /organism="Prosthecobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2804_length_8437_cov_23.153424"
                     /isolate="AMR_MDS_5629"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1965333"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..471)
                     /locus_tag="PLP58_18055"
     CDS             complement(<1..471)
                     /locus_tag="PLP58_18055"
                     /inference="COORDINATES: protein motif:HMM:NF015298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septal ring lytic transglycosylase RlpA family
                     protein"
                     /protein_id="HRJ10490.1"
                     /translation="MVPRRLFQTLLTVLLASCASQKPAWQEQGYASYVADAYTGRPTS
                     SGQLYHPQAYTAAHHSLPFGTIVTVKNTQTGRVVNVTVNDRFPYYPGRVINLSSIAAQ
                     HIGIPYMSMAPVTVTAQSLPPGYGPAPQQHTAWQAPAAPAPQPAWQPQPAPYAAP"
     gene            complement(529..1938)
                     /locus_tag="PLP58_18060"
     CDS             complement(529..1938)
                     /locus_tag="PLP58_18060"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase"
                     /protein_id="HRJ10491.1"
                     /translation="MRLTTLLSLMAAFSLHAADRPDVLFIAVDDLNDWTSHLGGHPQA
                     RTPNIDRLVARGTSFTNTQCAAPACNPSRAALMSGMRPWQTGIYTNGDPAQGVLKDTL
                     TLNRHFLASGYNCQGGGKIYHGGGSEGRKDTWTKWMDRFPSIPEKEQNYNGLKSGHFD
                     WGPVDAKPDDMGDHKLTTWAVEQLQNAPADQPIFLAVGYVKPHLPWYVPREYFDRFPL
                     DTIQLPHVLEGDLADVPPAGVSMAKPQGDHAAVIQGGQWAKAVQAYLATVSFLDDQVG
                     RLLDGLDASPRKDKTLIVWWTDHGWHLGEKEHWRKFALWEEATRTDFAIVAPGIGKPG
                     QKCAAPVDYLSVYPTLCALTGLPVPAHVKGPSLLPLLKDPAASWPHLSVCTHGRGNHA
                     VRDTRWRHIRYADGSEELYDHAADPYEHKNLAADPAHAAEKVRLAAAMPALKDEVPSI
                     SRAEKAAEKKPGKKKKTAG"
     gene            2125..3303
                     /locus_tag="PLP58_18065"
     CDS             2125..3303
                     /locus_tag="PLP58_18065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009964438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit H"
                     /protein_id="HRJ10492.1"
                     /translation="MAVFLASFDLALLVTSVVKIVFLTFLVIMPMVAYSVYAERRFSA
                     VIQDRVGPNRVGIPLTLFGFKKDIQILGIGGLVQPLADGLKFFLKEDFTPRTVNTFYY
                     WLAPCLTIVPALMTCAVLPFGSELDLSIIGLEQPVKAVIADLNVGPLFVFAIASLGVY
                     GIVIAGWSSNSKYPFLGGVRSSAQMISYEISLGLSIIPVLMIFGELNLSKMSAFQDAN
                     GWLLLPLWGEGLSLERWVLLVPVVISFCVFVVAMFAETNRLPFDMAECETELVAGYHT
                     EYSSMKFALFFMGEYAAMIIGSGMAVTMFLGGWSIPFAPLLGLDYKPGDTPLWLGLLH
                     IGTFFAKIVGFILFFILIRWSLPRFRFDQLMKLGWLFMFEVALANVVLTALILIAFNS
                     "
     gene            3319..3864
                     /locus_tag="PLP58_18070"
     CDS             3319..3864
                     /locus_tag="PLP58_18070"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009964437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit I"
                     /protein_id="HRJ10493.1"
                     /translation="MATITVQRPKLAWHEKLFLSTLVKGLKITMGHAIRTFRQIAGKK
                     EKVTMEYPEQKWDENLPEYYRGAPALVTDEHGRERCVSCQLCEFICPPKAIKITPGEI
                     PSDDPWAKVEKRPKEFEIDMIRCIYCGMCEEVCPEQAIYLRKDYAITGESRAEMVHDK
                     KRLYEIGGMRVGLVNKWNELK"
     gene            3912..4481
                     /locus_tag="PLP58_18075"
     CDS             3912..4481
                     /locus_tag="PLP58_18075"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009964436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit J"
                     /protein_id="HRJ10494.1"
                     /translation="MPPLLFYLFALITLGFGLLVVTARNPVTSALSLAMSFVGLAALF
                     LSLDAYFIGIIQILVYAGAVMVLFLFIIMLLDIKAEEGKTFNLKAVVGGIVLAAILAV
                     QVATIASSYQPGKTRLDEAKLDLKLAADYSKLPTIANDLRAGTLPDAKLVGQSLFQRY
                     GFHLQVIGLLLLVGTVGVVVLSKREREAN"
     gene            4483..4800
                     /gene="nuoK"
                     /locus_tag="PLP58_18080"
     CDS             4483..4800
                     /gene="nuoK"
                     /locus_tag="PLP58_18080"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009964434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoK"
                     /protein_id="HRJ10495.1"
                     /translation="MITLSHYLIVSAMLFALGLAGVVARRNILIIYMCLEMMLSAANL
                     TLVAFTRFHVTDGLPDFAAQSLVFFTITVAAAEVAVGLAIIVALYRAKQSVETEAVTK
                     LQG"
     gene            4815..6728
                     /gene="nuoL"
                     /locus_tag="PLP58_18085"
     CDS             4815..6728
                     /gene="nuoL"
                     /locus_tag="PLP58_18085"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009964433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit L"
                     /protein_id="HRJ10496.1"
                     /translation="MPAQETSHATLPTLLLLLPFFTALAILLGHRLLKGAGVMLSLFS
                     AAACFLISMMMLGSNTTGDVVPLLPFIEIGKFRFAIDALIDQQCRGMMFIVTSIGLLV
                     HVFSLGYMKQDAGKVRFFGALSLFMFSMTGIVLAGNLVMMFVFWELVGLSSYLLIGHW
                     FEKASAADASKKAFLTNRIGDFGFMIGILMLFAANHGNVSLAGADGLREALTSGTLHD
                     IAQTQGTYMTMAVLCLFMGAVGKSAQFPLHVWLPDAMEGPTPVSALIHAATMVAAGVY
                     MLVRVAFLIEAAPVAAQTIAWVGGITALMAALMATQQNDIKRVLAYSTLSQLGYMVMA
                     VGLVAMQHAGAHHEVVPGHPGMFHLYTHAFFKAMLFLGAGAVIYSCHHEQDMWKMGGL
                     MKHMPVTFITFAIGTASLMGVPFITSGFWSKEAILGVAFEVKGGSPLFWIGVATAMLT
                     AFYMTRLFVVAFLGKPRTESAHHATEAPVVMLLPLLVLAGLTLISAVPGFARLLEAMT
                     PHHAEEHMTVMIASILALVIGAGGGFYLYKGKDKDPLNIRLFANKFYWDEIYAVLVKV
                     FQDTLAWIVAGLDRLLVDGIFSRLPAFLAGRIGSAARALQGGHLQGYTFLLGLGAVLV
                     IYLVVFVLPAAGH"
     gene            6730..8226
                     /locus_tag="PLP58_18090"
     CDS             6730..8226
                     /locus_tag="PLP58_18090"
                     /EC_number="1.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018969134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit M"
                     /protein_id="HRJ10497.1"
                     /translation="MSILEVVILLPVLAALAVWLGAPARLVSVGSALLNLVILLGVAA
                     KYKAAAATGGLAFQHARVVLENPAISFGVGVDGTALILGLLTALVTLAAVWQISDDRP
                     PMYHIASQLIAGGAFGAFLCTDVFFIYAFHELALIPTFLMIALHGRADDATRKAVAWK
                     ATIYLGAGSLVLLAGLGWLVLEFSGGSKLTFDLIALREMAQSSPLPVEKQGVIFFVLL
                     IGFGTLVSLFPLHSWAAPAYATAPVPVAMLHAGVLKKFGLYGLIRIALPLLPQGPHVE
                     WVQKAMLLMLLGNILIIGLVTIAQKSLDRILGNSSVMHMGYIFLGIAAGTELALQGAV
                     LLMFGHGIAIALLFALTGRMRNQFGTLEVAKLGGLASHAPVFSILFAFGAFASIGLPG
                     LANFAGEVLIFLGAFGHDGTATVGPLQWTVILALWGVVMSAVYMLRAYRGIFQGTAVT
                     GLFMNDPSFSQRVPLFLLAAALLVVGCCPWLLLDFLKSLSPTAAVVAM"
     gene            8248..>8437
                     /locus_tag="PLP58_18095"
     CDS             8248..>8437
                     /locus_tag="PLP58_18095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ10498.1"
                     /translation="MSVFSIEILLVVLGVGLLLLEALVPTLSRRALASISLGGAALAL
                     VLFFFADKSAAALPAFVQP"
BASE COUNT         1288 a         2593 c         2713 g         1843 t
ORIGIN      
        1 aggcgcggca tagggagcgg gctgcggctg ccacgcgggt tgaggagccg gggcggcggg
       61 cgcctgccag gcggtgtgtt gctgaggggc ggggccgtaa ccgggcggca ggctctgcgc
      121 ggtgaccgta actggcgcca tgctcatgta ggggatgccg atgtgctggg cggcgatgct
      181 ggagaggttg atgacgcgcc ctggataata ggggaatcgg tcattgacgg tgacgttcac
      241 cacgcgcccg gtctgcgtgt ttttgacggt gacgatggtg ccgaagggca gggaatgatg
      301 cgcggcggtg taggcctggg ggtggtagag ttggccggag gaagtgggcc tgccggtgta
      361 agcgtccgcc acataagagg cgtaaccctg ctcctgccag gcaggttttt gactggcgca
      421 ggaggccagc agcacggtca gcagggtctg gaaaagtcgg cgtgggacca tcgtggcttg
      481 gttatacacc gtcggcggcg gcgcgtcatg cgtgaaaatg gcggcgggtt acccggccgt
      541 ttttttcttt ttgcccggct ttttctcagc ggctttttca gctctggaga tggaggggac
      601 ttcgtctttg agggccggca tggcggcggc gaggcggacc ttttcggcgg cgtgggcggg
      661 gtcggcggcg aggtttttgt gctcgtaggg gtcggcggcg tggtcgtaga gctcttcgct
      721 gccgtcggcg tagcggatgt gacgccagcg ggtgtcgcgc acggcatggt tgccgcgacc
      781 gtgggtgcag acgctgaggt ggggccagga cgcggcgggg tctttgagca ggggcagtag
      841 actggggcct ttgacatggg cgggcacggg caggccggtc agggcgcaga gggtgggata
      901 gacgctgagg tagtccactg gggcggcgca tttttggccg ggcttgccga tgccgggggc
      961 gacgatggcg aagtcggtac gggtggcctc ctcccagagt gcgaatttcc gccagtgctc
     1021 tttttcaccg aggtgccagc cgtggtcggt ccaccagacg atgagggttt tgtccttgcg
     1081 cgggctggcg tcgagcccgt cgagcaggcg gccgacctgg tcgtcgagga aggagacggt
     1141 ggcgaggtag gcctggacgg ctttggccca ctggccacct tggatgacgg cggcgtggtc
     1201 gccctggggc ttggccatgc tgacaccggc ggggggcaca tcggcgaggt cgccctccag
     1261 cacatgcggg agctggatgg tgtcgagggg gaagcggtcg aagtattcgc gcgggacata
     1321 ccaggggaga tggggcttca catagccgac ggcgaggaag attggctggt cagcgggggc
     1381 gttctggagc tgttccacgg cccatgtggt gagcttgtgg tcgcccatgt cgtcgggttt
     1441 ggcgtccacg gggccccagt cgaaatggcc gcttttgaga ccgttgtagt tttgctcctt
     1501 ctccgggatg ctggggaagc ggtccatcca ctttgtccag gtgtctttgc ggccctcgct
     1561 gccgccgccg tggtagattt tgccgccgcc ctggcaattg taaccgctgg cgaggaagtg
     1621 gcggttgagg gtgagagtgt ctttgagcac gccctgggcg gggtcgccat tagtgtagat
     1681 gccggtctgc caggggcgca tgccgctcat gagggcggcg cgggaggggt tgcaggcggg
     1741 cgcggcgcac tgggtgttgg tgaaggaggt gccgcgggcg acgaggcggt caatgttcgg
     1801 cgtcctggcc tgcgggtggc cgccgaggtg gctggtccaa tcgttgaggt catccacggc
     1861 gatgaagagg acgtcgggcc ggtccgccgc gtggagggaa aaggcggcca tcagggagag
     1921 gagggtggtc agtctcatga ggcgctggga acgcgggcaa ggatggcatc ttgcgtgctg
     1981 tgtacggcag gcgggcgggc ggcggcgtgt gccgcccttt ctcagggcat ggagcgcccg
     2041 caaaggggcc tggcagcgag cttgtgaaat ttttcacaaa ttcgggttgc cgctcgtaac
     2101 cgcatcctga taatgccgcg ccccatggct gttttcctcg ccagcttcga tctcgccctt
     2161 cttgtcacct cggtggtcaa gatcgtattt ctcacctttt tggtcatcat gcccatggtc
     2221 gcctactccg tgtatgcgga gcggcgcttt tcagcggtga tccaggaccg cgtggggccg
     2281 aaccgggtgg gcattccgct gacgttgttc ggattcaaaa aagacatcca gattctcggc
     2341 atcggcggtc tggtgcagcc gctggcggac gggctgaaat tcttcctcaa ggaggacttc
     2401 acgccgcgca cagtgaacac tttttattac tggctggccc cgtgcctgac catcgtgccc
     2461 gcgctcatga cctgcgcggt gctgcccttt ggcagtgagc tggacctttc catcattggc
     2521 ctggagcagc cggtgaaggc ggtcattgcg gacctgaatg tggggccgct gtttgtcttc
     2581 gccatcgcct ccctgggggt gtacggcatc gtgatcgccg gctggtcttc gaactcgaag
     2641 tatcccttcc tcggcggcgt gcgctccagc gcgcagatga tctcatatga aatctcgctc
     2701 ggcctttcga tcatccccgt gctgatgatc ttcggcgagc tgaacctctc gaaaatgtcc
     2761 gctttccagg acgccaacgg atggctgctg ctgccactct ggggcgaggg gttaagcctg
     2821 gaacgctggg tgctgctggt gccggtggtc atttccttct gcgtctttgt cgtggccatg
     2881 ttcgccgaga cgaaccgcct gccctttgac atggcggagt gcgagacgga actcgtggcc
     2941 ggctaccaca cagaatacag ctccatgaag ttcgcgctct ttttcatggg cgagtatgcc
     3001 gcgatgatca tcggctccgg catggcggtg acgatgttcc tcggcggctg gagcattcct
     3061 ttcgcaccac tgctcggcct cgactacaag cctggggaca ccccgctctg gctcggcctg
     3121 ctgcacatcg gcaccttctt cgccaagatc gtcggcttca tccttttctt catcctcatc
     3181 cgctggtcgc tgccgcgctt ccgcttcgac cagctcatga agctgggctg gctgttcatg
     3241 tttgaggtgg cgctggccaa tgtcgtgctc accgccttga tcctcatcgc cttcaattcc
     3301 tgatccccac cgcgactcat ggccaccatc accgttcagc gccccaagct cgcctggcac
     3361 gaaaagctct ttctttccac cctggtgaaa ggtttgaaaa tcaccatggg ccacgccatc
     3421 cgcaccttcc ggcagattgc cggtaaaaag gagaaggtga cgatggaata ccccgaacag
     3481 aagtgggatg aaaacctgcc ggaatactac cgcggcgctc ccgcgctggt caccgacgag
     3541 catggccgcg agcgctgcgt gtcctgccag ctttgcgagt tcatctgccc gccgaaggcc
     3601 atcaagatca cacccggcga aatccccagc gacgatccct gggccaaggt ggagaagcgg
     3661 ccgaaggagt ttgagataga catgatccgc tgcatttact gcggcatgtg cgaggaggtc
     3721 tgccccgagc aggctattta tctgcgcaag gattacgcga tcaccggcga gtcccgcgcc
     3781 gagatggtgc atgacaagaa gcgcctctat gaaatcggcg gcatgcgcgt cggcctcgtg
     3841 aacaagtgga acgagctgaa gtgaccgggg aagtttgaaa tccgaaattc gaaagacgaa
     3901 gcctgtatcc aatgccgccc cttttgtttt atctcttcgc cctgatcacc ctcggcttcg
     3961 gcctgctggt ggtcacggcg cgcaacccgg tgacgagcgc cctgagtctg gcgatgagtt
     4021 ttgtgggcct ggccgcgctg tttttgagcc tcgacgccta tttcatcggc atcattcaga
     4081 tactcgtcta tgccggcgct gtgatggtgc tttttctgtt catcatcatg ctgctcgaca
     4141 tcaaggcgga ggagggcaag accttcaacc tcaaggcggt cgtgggcggc attgtgctgg
     4201 cggccatcct ggcggtgcag gtggccacca tcgccagcag ttaccagcca ggtaaaaccc
     4261 gccttgatga ggcaaagctc gatctcaaac tggcggcgga ttattcaaag ctgcccacca
     4321 tcgccaacga cctccgcgcc ggaacgctgc ctgatgccaa gctggtaggg cagagtcttt
     4381 tccagcgcta cggcttccac cttcaggtca tcggcctgct gctgctggtg ggcacggtgg
     4441 gcgtggtggt tttgagcaaa cgagaaaggg aggcgaactg acgtgatcac cctcagccat
     4501 tacctcatcg tcagcgccat gctcttcgcc ctcggcctcg cgggcgtggt ggcgcggcgg
     4561 aacatcctga tcatctacat gtgcctggag atgatgctca gcgcggccaa cctgaccctg
     4621 gtggccttca cccgctttca tgtcaccgac ggcctgccgg acttcgccgc gcagtcgctc
     4681 gtgttcttca ccatcaccgt cgctgccgcc gaggtggccg tcggcctcgc catcatcgtg
     4741 gcgctctacc gcgcgaagca gagcgtcgaa accgaagccg tgacgaaact gcaaggctaa
     4801 gcgccccgcc ccccatgccc gcccaggaaa cctcccacgc cacccttccc acgctgctgc
     4861 tgctgctgcc gtttttcacg gcgctcgcca tcctgctggg gcatcgccta ttgaaaggcg
     4921 cgggcgtcat gctctcgctc ttttccgcag ccgcctgctt cctcatcagc atgatgatgc
     4981 tcggttcaaa cacgaccgga gatgtcgtcc cgctgctgcc cttcatcgag atcggcaagt
     5041 tccgcttcgc cattgacgcg ctgattgacc agcaatgccg cggcatgatg ttcatcgtca
     5101 cctccatcgg actgcttgtg catgttttct cactcggcta catgaagcag gatgcgggga
     5161 aagtacgctt cttcggcgcg ctctcgctct tcatgttctc catgaccggc atcgtgctgg
     5221 cggggaacct cgtcatgatg ttcgtctttt gggaattggt gggcctcagc tcctaccttc
     5281 tcatcggtca ttggtttgaa aaggccagcg ccgcggatgc ctcgaagaaa gccttcctca
     5341 ccaaccgcat cggcgatttc ggtttcatga tcggcatcct catgctgttc gcagccaatc
     5401 acggcaacgt cagcctggct ggcgctgacg gcctgcgcga ggcgctgacc agcggcacgt
     5461 tgcacgacat cgcccagacc cagggcacct acatgacaat ggccgtgctt tgcctcttca
     5521 tgggcgcggt gggcaagtcg gcgcagtttc cgctgcatgt ctggctgccg gatgcgatgg
     5581 aaggccccac gccagtgtcg gcgctcatcc atgcggcgac gatggtggcc gccggtgtct
     5641 acatgctcgt gcgtgtggcc ttcctcattg aggcggctcc agtggcggcg cagacgatcg
     5701 cctgggtcgg cggcatcacc gctctcatgg ccgcgctcat ggccacgcag caaaatgaca
     5761 tcaaacgcgt gctggcctac tccacgctct cgcagctcgg ttacatggtc atggccgtcg
     5821 gtctggtggc catgcagcac gctggcgcgc atcacgaggt ggtgccgggg catccgggca
     5881 tgttccacct gtacacacat gcctttttca aagccatgct cttcctcggc gcgggcgcgg
     5941 tcatttactc ctgccatcac gagcaggaca tgtggaaaat gggcggcctg atgaagcaca
     6001 tgcccgtgac ctttatcacc ttcgccatcg gcacggcgtc cctcatgggc gtgcctttca
     6061 tcacgagcgg attttggagc aaggaggcca tccttggcgt ggccttcgag gtcaaaggag
     6121 gcagcccgct tttctggatc ggtgtggcca cggccatgct cacggccttt tacatgacgc
     6181 ggctgtttgt cgtggccttc cttggcaaac cgcgcacaga gtcagcccat cacgccaccg
     6241 aggcgccggt tgtcatgctg ttgccgctgc tggtgctcgc gggtctcaca ctgatctccg
     6301 cagtgccggg attcgcacgt ttgctggagg ccatgacgcc tcatcacgcc gaggagcaca
     6361 tgacggtgat gatcgcctcc attctggccc tggtcattgg cgccggcggc ggtttttacc
     6421 tctacaaagg caaggacaag gatccgctga acatccgcct gtttgcgaac aagttttatt
     6481 gggacgaaat ctacgccgtg ctggtgaagg tcttccagga cacgctggcg tggatcgtcg
     6541 ccggtctcga ccgcctgctg gtggacggca ttttctcccg ccttccggcc ttcctcgccg
     6601 ggcgcatcgg ctccgcggcg cgcgctctcc agggcgggca cttgcagggc tacaccttcc
     6661 tgctgggcct gggcgccgtc ctggtcatct accttgttgt gtttgtcctg cccgcagcgg
     6721 gccattgata tgagcattct cgaagtcgtc atccttctgc ccgtcctcgc cgcgctggcc
     6781 gtgtggctgg gcgcgcccgc gcgccttgtc tccgtgggct cggccttgct gaaccttgtc
     6841 atcctcctcg gtgtggcggc gaaatacaaa gcggcggctg ccacaggggg cctcgctttt
     6901 caacacgccc gagtggtgct ggaaaacccc gccatcagct tcggtgtcgg cgtggatggc
     6961 acagccctca tcctcggact gctgaccgcg ctggtcacgc tggctgctgt ttggcagatc
     7021 tcggatgatc gtcctccgat gtatcacatc gcctcccagc tcatagcggg cggggctttt
     7081 ggcgcgtttc tttgcacgga tgtcttcttc atctacgctt tccacgaact ggcgctgatc
     7141 cccaccttcc tcatgatcgc tctgcatggc cgggccgatg acgcgacgcg caaggcggtg
     7201 gcctggaagg cgacaattta ccttggcgcg ggcagtctcg tgctgctggc cggtctcggc
     7261 tggcttgtgc tcgagttcag cggcggcagc aagttgacct tcgatctcat cgccctgcgc
     7321 gagatggctc aatccagccc gctgccggtg gaaaagcagg gcgtcatttt cttcgtgctg
     7381 ctgatcggct tcggcacgct ggtcagcctc ttcccgctgc atagctgggc cgcgcccgcc
     7441 tatgccacgg caccggttcc cgtggccatg ctgcatgcag gggtactgaa aaagttcggt
     7501 ctttacggcc tgatccgcat cgccctgccg ctgctgcccc aggggccgca cgtcgagtgg
     7561 gtgcagaagg ccatgctgct catgctgctg gggaacatcc tcatcatcgg cctcgtcacc
     7621 atcgcgcaaa aatcgctcga ccggattctg ggcaactcct cggtgatgca catgggctac
     7681 atcttcctcg gcatcgccgc aggcaccgag ctggctttgc aaggcgcggt attgctgatg
     7741 tttggtcacg gcatcgccat cgcgctgctc tttgccctca ccgggcggat gcggaatcag
     7801 ttcggcaccc tggaggtggc aaagctcggc ggcctggcct cgcacgcgcc ggtgttcagc
     7861 atccttttcg ccttcggagc cttcgcctcc atcggcctgc cgggacttgc gaacttcgcg
     7921 ggcgaggtgc tcatcttcct cggcgctttc ggtcatgatg gcaccgccac ggtcgggccg
     7981 ctccagtgga ccgtcattct ggcgctctgg ggtgtggtga tgtccgccgt ctatatgttg
     8041 cgcgcctacc gcggcatctt ccagggcacc gccgtcaccg ggcttttcat gaacgacccc
     8101 tcgttctccc agcgtgtgcc gctttttctg ctggcggcgg cgctgctcgt cgtcggctgc
     8161 tgcccctggc tgctgctgga ttttctgaaa tccctctccc ccaccgccgc tgtggtcgcg
     8221 atgtagaatt cccgagcctc tccgtccatg tccgttttct ccatcgaaat cctcctcgtc
     8281 gtcctcggcg tcggcctgct gctgctggag gccctcgtgc ccaccctttc acgccgcgcc
     8341 ctggcctcca tcagcctcgg cggcgccgcg ctggcgctgg tgctgttctt ttttgccgac
     8401 aaatccgccg ccgctctgcc ggcattcgtg cagccct
//
LOCUS       DAOMYJ010000223         8437 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Prosthecobacter sp. isolate AMR_MDS_5629
            NODE_2805_length_8437_cov_18.402171, whole genome shotgun sequence.
ACCESSION   DAOMYJ010000223 DAOMYJ010000000
VERSION     DAOMYJ010000223.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867850
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prosthecobacter sp. (wastewater metagenome)
  ORGANISM  Prosthecobacter sp.
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Verrucomicrobiaceae; Prosthecobacter.
REFERENCE   1  (bases 1 to 8437)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8437)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.30
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:34:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,742
            CDSs (total)                      :: 4,684
            Genes (coding)                    :: 4,676
            CDSs (with protein)               :: 4,676
            Genes (RNA)                       :: 58
            tRNAs                             :: 55
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8437
                     /organism="Prosthecobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2805_length_8437_cov_18.402171"
                     /isolate="AMR_MDS_5629"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1965333"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..629)
                     /locus_tag="PLP58_18100"
     CDS             complement(<1..629)
                     /locus_tag="PLP58_18100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ10499.1"
                     /translation="MKTALLLLLSLLFSCAAAPRSPASAPAAVRLTPAQLEKAGRRVW
                     QNECGGTVAGLTSWNSGEQFASLGIGHFIWYPAGRRGPFEESFPPLAAFLEARGAAMP
                     AWTRGPCPWPDKAAFDAAREDPRLRELRRILSSTIALQTDFILARLSRALPKMQAQAR
                     APAQVAAIFHGLAATPEGAFCLMDYVNFKGEGTAPAERYQGRGWGLLQVL"
     gene            823..1860
                     /locus_tag="PLP58_18105"
     CDS             823..1860
                     /locus_tag="PLP58_18105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009960852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ10500.1"
                     /translation="MADDVKISKRKAFLPVTEDLESYLDQYDRLYSLPATYADLRGFT
                     ESYPLDDKHGNATHWASVLYPQEVREHLYPRLTSIYSLLRTGDMQAVPHLFVERVDFC
                     EFGNSRPFRVRVVNQYNDNYDHFYLKVSDASRVYGLELEHLLSPNRINYLAHGGTLVE
                     EHIAGVPGDAFIRDHLDRPEVNRVRIAKEFVKFSERCFIRLLGDMRSYNYVIDITPDF
                     EDVQYRVRAIDFDQQSYEGRRNIYLPQFFKENNAVVWLCGKLLNLPTMQQYQDEERSL
                     IARRYLSEHQRLSALIECMKKNPREPQDKVIQLARELGEHHHTSRFDHCDSMGAIVET
                     NLEVALARHLI"
     gene            complement(1870..2259)
                     /locus_tag="PLP58_18110"
     CDS             complement(1870..2259)
                     /locus_tag="PLP58_18110"
                     /inference="COORDINATES: protein motif:HMM:NF018021.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallopeptidase family protein"
                     /protein_id="HRJ10501.1"
                     /translation="MSLGDEHIARLLRVAGGVIERAARRLPAEIREAARACVTEAEIM
                     ADCMEAGEDDLDEDLLGLFEGCSLADPEPDMPGHLPRIRLFVDNLWEHAGRDMGVFRE
                     EVRITWLHELGHYLGLDEDQVAALGLA"
     gene            complement(2256..2720)
                     /locus_tag="PLP58_18115"
     CDS             complement(2256..2720)
                     /locus_tag="PLP58_18115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ10502.1"
                     /translation="MKLTHAACSAAALLLAACASTAPTPQELDALERKVRAEHQAEFA
                     ALEERRRGGQISDWEHEHELGQLEKRVRNKVDTQLWSRHALAQSELKANDIPTPDRPV
                     SNVPPMAGGIQNSLYNSARQTGMGNQVMGNFMGDFGGSNFNSPNRAGTLYDP"
     gene            2859..4823
                     /locus_tag="PLP58_18120"
     CDS             2859..4823
                     /locus_tag="PLP58_18120"
                     /inference="COORDINATES: protein motif:HMM:NF017280.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetylxylan esterase"
                     /protein_id="HRJ10503.1"
                     /translation="MKKLLLLSLLAGHLHAQSPAREPRDLNGYFPMTPPASKEAWQAR
                     RAEIEQRVALASGLWPIPEKTPLNAVVHGRVERDDYTIDRVFFESLPGHYVTGSLYLP
                     KQRPAKMPAILCPHGHWPDGRFMDRGTGTAATKKEIETGAEELECGARSPLQARCVQL
                     ARMGCAAFLYDMLGYADSVQFTEHRHGARAEGFLSPQADLRLQTYFGLQTWNGIRALD
                     FLLTLEGVDPARIGCTGASGGGTQTMMIAGLDSRVAAAFPCVMVSTAMQGGCTCENSH
                     HLRVNQGNIDIAALTAPRPLGLAAANDWTKELETKGLPELKALYSTLGVPDNVTAHIA
                     THFPHNYNLPSRLSMYRFYNQHFQLGIQEPVREREFQVEDKAALTVWTGAHPAPSGDQ
                     TGAAHEKTVCAWMTAQDEKLIQPLVKGADEAKLREIVGGAWKVIIGREPPAASEVEYD
                     LLTKTDRGDHLVMEGTARLATHGEEVPSTFLYPKNWSGHAVLWLCLEGPASILGENGP
                     APAARALLDEGKAIACPALHLQGATGQPKSGDNLRAKEDDFREFSGYTFGYNPSLLAR
                     RVHDVMTMITLMRNQEKYPLKSLEVRGLEGAGPIALAAGAVMGLKNVKADLQGFRFAA
                     LDNPWHIHFVPGAVKYGDVEALLKLCGEAE"
     gene            5086..5379
                     /locus_tag="PLP58_18125"
     CDS             5086..5379
                     /locus_tag="PLP58_18125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ10504.1"
                     /translation="MLVCFAGLRVWSALPDIQDGTAANDLAEKVLEQRADENHPGNLT
                     LPQGAKDCLDEALPDLARGAVAAEFFHAPSLPARRGSCLPCRVLNRGPPGMAV"
     gene            5570..5716
                     /locus_tag="PLP58_18130"
     CDS             5570..5716
                     /locus_tag="PLP58_18130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ10505.1"
                     /translation="MNDLLMNALRIGLGLWLLWILWLLIRAFAGQGRDPDEVRKQSLN
                     QDTH"
     gene            5785..6810
                     /locus_tag="PLP58_18135"
     CDS             5785..6810
                     /locus_tag="PLP58_18135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009962068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HRJ10506.1"
                     /translation="MLFPFAEVWWVYALFVAFVLGMLALDLGIFHRHAREVGVREAAF
                     WSAFWISLAVVFGAGLYFFSLDWFAREGALAAFPHLTPQSAARQVTLEFATGFLVEKS
                     LAVDNLFVFIVIFNYFAIPPVYQYRVLFWGIIGALVFRSIFIALGAALMQFHSVVILF
                     GVFLIFTGMKLVFSREEPRDLEQNRVFRLLRRVLPVWPRLEGQRFFVRQSGVLHATPL
                     LVALCFIEVSDIVFAVDSVPAIFALTREPFIVFTSNIFAILGLRALFLLVAKIMPQMH
                     YLKFGLAGVLVFVGLKMVWLNQKFGGQFPIAWSLGIIAVLIGGSLALSWLLPARGHAC
                     REGEKAS"
     gene            6817..7416
                     /gene="rfaE2"
                     /locus_tag="PLP58_18140"
     CDS             6817..7416
                     /gene="rfaE2"
                     /locus_tag="PLP58_18140"
                     /inference="COORDINATES: protein motif:HMM:TIGR02199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycero-beta-D-manno-heptose 1-phosphate
                     adenylyltransferase"
                     /protein_id="HRJ10507.1"
                     /translation="MSAPDFQPLTAEDVRRLRDEMDAAGKKLVFTNGCFDLLHAGHVR
                     YLNQARALGDAMVVAVNSDASVRALKGPARPVNNENDRAEVLAALRAVDAVVVFDDER
                     ATRLISAIRPHIYAKGGDYTVESLNPEERAALDAAGTAIHILPLVPGRSTTATLKRAA
                     TPEKSARLRLGVRRLSGQRCPGRGCGRRARRKWPTVSGG"
     gene            complement(7340..>8437)
                     /locus_tag="PLP58_18145"
     CDS             complement(7340..>8437)
                     /locus_tag="PLP58_18145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ10508.1"
                     /translation="SASDTTGPRHAWGVNTLIAHDDYMALRQGGDTLRELKKEQNPAA
                     QALHAVWSPNAQGFARRETGADGYLGKDAEDLVKKDPGHIQDRHLRPCVTLARPWSMW
                     LGVLAGDFSPARSGDSLSWGTGNPAGLFYGPALMTWRPGNGSGANEEAWVDFLSHLPG
                     HGRLLALGEDGRKDDKDENAGWLKGRHAADQNLAALDPGLPFYVPAHFATRMSLVPMR
                     HFVSDLAVDFHQNNHESSWLRFKTALNPAIGGATPPGPDGDQSAAKKLSGAGFPGGWH
                     QSGVYYHAPMALLTNQAGVSANAFTACFVVQAVRDNGKSREGIANSGPGHCDPDDEIL
                     AERWSRVILQKQSATSGAPAFHILFQDTAGR"
BASE COUNT         1409 a         2738 c         2785 g         1505 t
ORIGIN      
        1 agcacctgca gcaggcccca gccgcggccc tggtagcgct cggcgggggc tgtgccctcg
       61 cctttgaagt tcacatagtc catgaggcaa aaggcgccct ctggcgtggc tgccaggccg
      121 tgaaaaatgg cggcgacttg cgcgggcgcg cgcgcctggg cctgcatctt cggcagcgcg
      181 cgagagaggc gggcgagtat gaagtcagtc tggagggcga tggtggagga aaggatgcgg
      241 cgcagctcgc gcaggcgcgg gtcctcgcgc gcggcgtcaa aggctgcctt gtccggccag
      301 gggcaggggc cgcgcgtcca ggcgggcatg gccgcgccgc gcgcttcgag aaaggcggcg
      361 agaggcggga aactctcctc aaaggggccg cgccgcccag cgggatacca gatgaagtgg
      421 ccgatgccca gggaggcgaa ctgctcgccg ctgttccagc tcgtgagtcc ggccacggtg
      481 ccgccgcatt cattctgcca gacgcgccgg ccggctttct ccaactgggc gggcgtgagg
      541 cggacggctg cgggcgcgga ggccggcgac cgcggagcgg cggcgcagga gaaaaggagg
      601 ctgagcagca agagcagggc ggttttcatg cgggatgcgg tcgccacgct acgccggaat
      661 gacgcgggcg caaaaactgt gacacagaca ctcctgtcag tgagaactgg actggctgcc
      721 ccgggaaaac cagcgcggac aagtggtgtc cgcgtcacaa ctcccctgcc ccgaagaaac
      781 cggcgctgga cggcacgcgt catgcgctga tgatggacgg gcatggctga tgatgtgaag
      841 atttcgaaac gcaaggcatt cctcccggtc acggaggatc tggagagtta cctggatcaa
      901 tacgaccggc tttactcgct gccggcgact tacgcggacc tgcgcggctt cacggagagc
      961 tacccgctgg atgacaagca cgggaatgcc acgcactggg ccagcgtgct gtacccgcag
     1021 gaagtccggg agcatcttta tccgcgcctg acgagcatct actcgctgct gcgcacgggg
     1081 gacatgcagg cggtgccgca tttgtttgtg gagcgggtgg atttttgcga attcgggaac
     1141 tcgcgcccct tccgggtgcg agtggtaaac cagtacaatg acaactacga ccacttttat
     1201 ctcaaggtgt ccgacgcgtc gcgcgtgtac ggcctggagc tggagcatct gctgtcgccg
     1261 aaccggatca attacctggc gcacggcggc acgctggtgg aggagcacat cgccggcgtg
     1321 ccgggggacg ccttcatccg cgatcatctc gaccgcccgg aggtgaaccg ggtgcgcatc
     1381 gcgaaggagt tcgtcaaatt cagcgagcgg tgcttcatcc gcctgctggg ggacatgcgc
     1441 agctacaact atgtgataga catcacgcct gacttcgagg acgtgcaata ccgggtgcgg
     1501 gcgattgatt tcgaccagca gagctacgag gggcggcgga acatttacct gccgcagttt
     1561 ttcaaggaga acaacgcggt ggtgtggctg tgcgggaagc tgctgaacct gccgacgatg
     1621 cagcagtacc aggacgagga gcgcagcctg atcgcacgcc gttacctttc ggagcaccag
     1681 cggctgtcgg cgctcattga gtgcatgaaa aaaaatccgc gcgagcccca ggacaaggtc
     1741 atccagctcg ccagagaact gggcgagcac catcacacga gccgctttga ccactgcgac
     1801 agcatgggcg cgatcgtgga gacgaacctc gaggtggcgc tggcgcgaca tttgatctga
     1861 caggcccgct cacgccaggc cgagcgcggc cacctggtcc tcgtcgaggc cgaggtagtg
     1921 ccccagctcg tgcagccagg tgatgcgcac ctcctcgcgg aagacgccca tgtcgcgccc
     1981 ggcatgttcc cagagattgt ccacaaacag gcggatgcgc ggcaggtggc cgggcatgtc
     2041 cggctccgga tcggccaggg agcagccttc aaagaggccg agcaggtcct catcgaggtc
     2101 atcctcgccc gcctccatgc agtcggccat gatctcggcc tcggtgacgc aggcccgcgc
     2161 ggcctcccgt atttcggccg gcagacggcg cgcggcgcgc tcaatgacac ccccggccac
     2221 gcgcaggagg cgggcgatgt gctcgtctcc gaggctcatg gatcgtaaag ggtgcctgcg
     2281 cggttgggtg agttgaagtt gctgccgccg aagtcgccca tgaagttgcc catgacctgg
     2341 tttcccatgc cagtctgccg ggcgctgttg tagaggctgt tctggatgcc gcccgccatc
     2401 ggcggcacat tggacacggg tcggtcaggg gtgggaatgt cgttggcctt cagctcgctc
     2461 tgcgccaggg cgtggcggct ccagagctgg gtgtccacct tgttgcgcac ccgtttttca
     2521 agctgcccca gctcatgctc gtgctcccag tcgctgatct gcccgccgcg cctgcgctcc
     2581 tcaagcgcgg cgaactcggc ctggtgctcg gcgcgcactt tgcgctccag cgcgtcaagc
     2641 tcctgcggcg tgggggcggt gcttgcgcag gcggccagca gcagtgcggc ggcggaacag
     2701 gcggcgtgag tgagtttcat cgggcgactc taaaacggcg cgcgcgcggg ttcaatgcaa
     2761 accctgccgc gccggctgag cgcccgctac cggcaaagag atgggcgcgg caagattgtt
     2821 cttccatcac cagcgcgtac tgcctcatga tcgccatcat gaaaaagctg ctcctccttt
     2881 ccctcctcgc cggtcatctg cacgcccagt ctcccgcacg cgagccgcgc gacctcaacg
     2941 gctacttccc catgacgcca cccgcgtcca aggaggcctg gcaggcgcgg cgggcggaga
     3001 tcgaacagcg cgtggccctg gcttccggcc tctggcccat tccggaaaag acgccgctga
     3061 atgccgtggt gcatggccgc gtggagcggg acgactacac aattgaccgc gtctttttcg
     3121 agagtcttcc cggccactat gtcaccggca gcctttatct tccgaagcag cgcccggcca
     3181 aaatgcccgc catcctctgc ccgcacgggc actggccgga cgggcgcttc atggaccggg
     3241 gcacgggcac ggcggccacg aagaaggaga tcgagaccgg cgcggaggag ctggaatgcg
     3301 gcgcgcgctc gccgcttcag gcgcgctgcg tgcagctcgc gcgcatgggc tgcgcggcct
     3361 tcctctatga catgcttggt tacgcggact ccgtccagtt caccgagcac cgccacggcg
     3421 cgcgcgcgga gggtttcctc agcccgcagg cggacctgcg cctgcaaacc tacttcggcc
     3481 tgcaaacctg gaacggcatc cgcgcgctcg acttcctgct caccctggaa ggcgtggacc
     3541 ccgcgcgcat cggctgcacc ggcgccagcg gcggcggcac ccagacgatg atgatcgccg
     3601 gcctggactc tcgcgtcgcc gccgcttttc cgtgcgtgat ggtctccacc gccatgcagg
     3661 gcggctgcac ctgcgagaac agccaccatc tgcgcgtcaa ccagggcaac attgacatcg
     3721 ccgccctcac cgcgccgcgc ccgctgggcc tcgccgccgc taatgactgg accaaggagt
     3781 tggagaccaa gggcctgccc gaactcaagg cgctctacag cacgctcgga gtgcctgaca
     3841 atgtcaccgc ccacattgcc acccatttcc cgcacaatta caacctgccc tcccggctgt
     3901 cgatgtatcg tttctacaac cagcatttcc agttaggcat ccaggagccc gtccgcgagc
     3961 gcgagttcca ggtcgaggac aaagccgcgc tcaccgtctg gaccggcgcg catcccgccc
     4021 cttccggcga tcaaaccgga gccgcccacg aaaagaccgt ctgcgcctgg atgaccgccc
     4081 aggatgaaaa gctcattcag cctctcgtga agggcgctga tgaggcgaag ctgcgggaga
     4141 tcgtcggcgg cgcatggaag gtcatcatcg gacgcgagcc gcccgccgcc agtgaggtgg
     4201 agtacgatct gctcaccaaa accgatcgtg gcgaccacct cgtcatggaa ggcacggccc
     4261 gcctcgccac gcacggcgag gaagtgccct ccaccttcct ttacccgaag aactggagcg
     4321 gccacgccgt gctctggctc tgcctcgaag gccccgcctc catcctcggc gaaaacggcc
     4381 ccgctcccgc cgcgcgcgcc ttgctcgacg agggcaaagc catcgcctgc cccgcgctcc
     4441 acctgcaagg cgccaccggg cagcccaagt ccggcgacaa cctgcgcgcc aaagaggacg
     4501 acttccgcga gttcagcggc tacacctttg gctacaaccc cagcctgctc gcgcgccgcg
     4561 ttcacgatgt catgacgatg atcacgctca tgcgcaatca ggaaaaatac ccgctcaaga
     4621 gcctcgaagt gcgcggcctg gaaggcgcgg gccccatcgc cctcgccgcc ggagcggtca
     4681 tgggattgaa gaacgtgaag gccgatctcc aaggcttccg cttcgccgcc ctcgacaacc
     4741 cctggcacat ccacttcgtc cccggcgccg tcaaatacgg cgatgtcgag gcgctgctga
     4801 agctgtgcgg agaggcggag tgaattcgtt ggagtgaagc cttcaggccg ttggagtgaa
     4861 gccttcaggc gacacgggag tggactccgc tgcgagagct ttcccagaac aagctaaagc
     4921 ttgaactcca atcctagaac tccaatcctt gaattcaaac ccgctctcac atcgcgaaga
     4981 atctcgtggc gcgcgtttga caactgcggc ttttcggcga cacacgccgc agtgccacgc
     5041 attcgccagt tttcccttgt tcagcgcccc gttttcgcgc tgtggttgct ggtttgcttc
     5101 gcgggtctgc gcgtgtggtc ggcgctgccg gacatccagg atggcactgc ggcgaatgac
     5161 ctcgccgaaa aggtgctgga gcagcgcgcg gatgaaaatc atcccggcaa tctgacgctg
     5221 ccacaggggg cgaaggactg cctggacgag gccttgccgg acctggcaag gggcgcggtg
     5281 gcggcggagt ttttccacgc ccccagcctg cccgcccgtc gaggctcatg ccttccctgc
     5341 cgtgtgctga accggggacc tcccggcatg gcggtgtgaa atcctgaact ccaggcgcgc
     5401 ccgctcaaga gcgcgaaaaa atcacggcgc cgcacgaacg gcgtcactcc gcccatggcg
     5461 gaaatcccca cccggccggc tgacacagcc gcgccacctc ccgcaggcag ccgcgtctga
     5521 aacgccgccc ctgcgggcaa ccccgctttg caaaacacac acacatttta tgaacgacct
     5581 tttgatgaac gccctgcgca tcggcctggg cctatggctg ctttggattc tttggctgct
     5641 gatccgcgcc tttgccggcc agggccgcga tcccgatgaa gtccggaagc agagcctcaa
     5701 ccaggacacc cactgagcac gcggctccgg gcggtgaact ccgccgcccg gagccctttc
     5761 tgaatctcca caaccgcatc cgccatgttg tttcccttcg ctgaagtttg gtgggtttac
     5821 gccctgtttg tcgcctttgt cctgggcatg ctggccctcg acctcggcat ttttcatcgt
     5881 cacgcccgcg aggtcggcgt cagggaggcc gcgttttgga gtgccttctg gatcagcctg
     5941 gccgtcgtct ttggcgctgg gctgtacttt ttcagtctcg actggttcgc gcgcgaaggc
     6001 gcgctggcgg cttttccgca cctgactcca cagagcgccg cccgacaggt gacgctggag
     6061 tttgccaccg gttttctcgt cgagaagtcg ctggccgtgg acaacctttt tgtcttcatc
     6121 gtcatcttca actacttcgc catcccgccg gtttaccagt accgggtcct gttctggggc
     6181 atcattggcg cgctcgtctt ccgctccatc ttcatcgccc tcggtgctgc gctgatgcag
     6241 ttccactcgg tggtgatcct gttcggggtg ttcctcattt tcacaggaat gaaactcgtc
     6301 ttctcccgcg aggagccgcg tgatctggag cagaaccgtg tcttccgcct gctgcgccgc
     6361 gtgctgcctg tgtggccgcg actggaggga cagcgcttct ttgtgcgcca gtccggtgtg
     6421 ctgcacgcca cgccgctcct ggtggcgctg tgcttcatcg aggtgtccga catcgtcttc
     6481 gcggtggact cggtgcccgc catcttcgcg ctgacgcgcg agccgttcat cgtcttcact
     6541 tcgaacatct tcgccattct cggcctgcgc gcgctgttcc tgcttgtggc gaaaatcatg
     6601 ccgcaaatgc actatctgaa gttcggcctg gcgggtgtgc tggtctttgt cggcttgaaa
     6661 atggtgtggc tgaatcagaa gttcggaggc cagtttccca tcgcctggtc cctgggcatc
     6721 atcgccgtgc tcattggcgg ctcgcttgcg ctgtcgtggc tgctgcccgc gcgcggccat
     6781 gcttgccggg aaggtgaaaa ggcttcataa aaccgcatgt ccgcccccga tttccagccc
     6841 ctgaccgccg aagatgtccg ccgcctgcgc gacgagatgg atgcggcggg taaaaagctg
     6901 gtgttcacca acggctgctt cgatctgctg cacgcgggcc atgtgcgtta tctcaatcaa
     6961 gcccgcgctc tgggagatgc gatggtggtg gccgtgaact cggacgcctc cgtgcgcgcg
     7021 ctcaaaggcc ccgcgcgccc ggtgaacaac gaaaacgacc gcgccgaggt cctcgccgcg
     7081 ctgcgcgccg tggatgcggt ggtcgtcttc gatgacgagc gcgccacgcg cctcatcagt
     7141 gccatccgcc cgcacattta tgccaagggt ggcgactaca ctgtggaatc gctcaatcca
     7201 gaggagcgcg ccgcgctgga tgcagccgga accgccatcc acatcctgcc tctcgtgccc
     7261 ggacgctcca ccacagccac tttgaagcgc gccgccacgc cggaaaaaag cgcgcgtctg
     7321 cgcctcggcg tgcggcggct cagcggccag cggtgtcctg gaagaggatg tggaaggcgg
     7381 gcgcgccgga agtggccgac tgtttctgga ggatgacgcg gctccagcgc tcggcgagga
     7441 tttcgtcatc gggatcgcag tggccgggac cggagtttgc gatgccttcg cgggacttgc
     7501 cgttgtcgcg cacggcctgg acgacgaagc aggcggtgaa ggcgttggcg ctgacgccgg
     7561 cctggttggt gagcagggcc atgggggcgt ggtaatagac gccggactga tgccagccgc
     7621 cggggaagcc ggcgccggag agctttttgg cggcggactg gtcgccgtcg gggccgggcg
     7681 gggtggcgcc gccgatggcg gggttgaggg cggttttgaa gcggagccaa ctgctttcgt
     7741 gattgttctg gtggaagtcc acggcgaggt cactgacgaa gtggcgcatg ggcacgaggc
     7801 tcatgcgtgt ggcgaagtgg gcggggacgt aaaagggcag gccgggatca agggcggcga
     7861 gattttggtc agcggcgtgg cggcctttga gccagccggc gttttcgtct ttgtcgtcct
     7921 tgcgcccgtc ctctccgagg gcgagcaggc gtccgtggcc gggcagatgg ctcaggaagt
     7981 ccacccaggc ttcctcattg gcgccggagc cgttgccggg acgccaggtc atgagggcgg
     8041 ggccgtaaaa gaggccggcg gggttgcccg tgccccagga gaggctgtcg ccggagcggg
     8101 cgggtgagaa gtcacccgcg agcacgccga gccacatgct ccaggggcgg gcaagggtga
     8161 cgcaggggcg gagatggcgg tcctggatgt ggccggggtc ttttttgacg aggtcttcgg
     8221 cgtccttgcc gaggtagcca tcggcgccgg tctcgcggcg ggcgaaaccc tgggcgttcg
     8281 gactccacac ggcgtgcaag gcttgggcgg cggggttttg ctcttttttc agctcgcgaa
     8341 gggtgtcgcc gccctggcgc agggccatgt agtcgtcgtg agcgatgagg gtgttcacgc
     8401 cccaggcgtg gcgcggaccg gtggtgtcgg aggcgct
//
LOCUS       DAOMYJ010000224         8300 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Prosthecobacter sp. isolate AMR_MDS_5629
            NODE_2869_length_8300_cov_24.565797, whole genome shotgun sequence.
ACCESSION   DAOMYJ010000224 DAOMYJ010000000
VERSION     DAOMYJ010000224.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867850
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prosthecobacter sp. (wastewater metagenome)
  ORGANISM  Prosthecobacter sp.
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Verrucomicrobiaceae; Prosthecobacter.
REFERENCE   1  (bases 1 to 8300)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8300)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.30
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:34:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,742
            CDSs (total)                      :: 4,684
            Genes (coding)                    :: 4,676
            CDSs (with protein)               :: 4,676
            Genes (RNA)                       :: 58
            tRNAs                             :: 55
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8300
                     /organism="Prosthecobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2869_length_8300_cov_24.565797"
                     /isolate="AMR_MDS_5629"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1965333"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..677)
                     /locus_tag="PLP58_18150"
     CDS             complement(<1..677)
                     /locus_tag="PLP58_18150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009962847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HRJ10509.1"
                     /translation="MSPAESYLQRSAALLERVRAQLPLIGQAADLFSRTILAGQMVHV
                     FGSGHSRILVEEMWPRYGSFPGFNPIVELSLTFHNLVVGANGQRQAMFLENVSGLAAR
                     ILRNFDLKAGDSALIVSSSGCNVVPVEMAEEFQRRGVKVVALISKDHAEASISRHPAG
                     KKLQDCADLVLDTGAPVGDAMVRIEGLDTPVAPGSTVGGCILVNAIKAEVAQRLTAAG
                     RPPRVLTA"
     gene            800..2341
                     /locus_tag="PLP58_18155"
     CDS             800..2341
                     /locus_tag="PLP58_18155"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HRJ10510.1"
                     /translation="MTLKHLLPAALCCLLVAMPLPAVEKRPNFLFILVDDQAPFDLKV
                     YDPKSPLDTPNLDRLAAQGVVFDGAYQMGSFSGAVCMPSRHMIMSGRTVWHLPPAPWG
                     AKTCPPNLEQNSIPAVFNRAGYATMRTCKTGNSYEAANKLFTVRHDASKRGGDDETGS
                     TWHGDRVMDYLRDRESAKDDKPFFIFYGFSHPHDTRDGRPELLAKYGAVNHTDEAAPP
                     SSHSLQPGLPPNYLPGHPFDMTHADVRDEVAVSGVWKRRDEASIRNEIGRQYACSENI
                     DIQIGRVLKRLEETGELDNTFIIYTADHGMSIGRHGLMGKQNLYQHTWRVPFIVKGPG
                     IPAGRRVEGNIYLLDILATFCDLAGIPAPETNEGLSFKPVLLGEKPVMRDVLYGAYSG
                     GSKPGIRTVKKGDWKLVRYESPDRSVNETQLFNLAENPDELLPQHHDPKVAALTGNMP
                     KPEQTNLASDAAHAAKLAEMQALLLAEMRRLNDPWRFSDQPDDNLPAPPAPQPRGKGK
                     GKAGK"
     gene            complement(2423..4231)
                     /locus_tag="PLP58_18160"
     CDS             complement(2423..4231)
                     /locus_tag="PLP58_18160"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HRJ10511.1"
                     /translation="MNRLLFALLLVFLCQLSAQARPNILWITSEDNSPYLGCYGDALA
                     QTPNLDKLAARGIRYRNAFSNAPVCSTARSTLITGMHACSTGIHNHRSSVPYPADFLM
                     YPEHLRAAGYYCVNNSKQDYNFAGKREVWDESSKNAHYRNRRPGQPFFAVFNFTTSHE
                     SQVAPKKGKDSFRIKPEEMPLPPYHPDTPDIRKDWANYYDQMTLMDAEAGKVLAELDE
                     LGEAKDTIVFYYGDHGGALPRGKRNIHDSGTRVPLIVHIPEQWKNWRPVEPGGWMEDP
                     VAFVDLPATVFSLCGVDIPAHYEGVPFLGEKKAAPREHVFLFRGRMDERYDTVRAVRD
                     RGFRYARNYSPHRPWGQHQTYAFQVQPSMRSWFAEFEAGRCNDAQSAYWKPKPPEELY
                     DTGADKHEIHNLIAEPGQAARIESMRAALRHEIMRLRDTGFIPEGMFPRLAGDKTIYE
                     YAQSNAYPLGPILDLADKATARDASKLPDLITALADPHPVKRYWAATGCLILQDQAVH
                     AKGGLLPLLKDEWPDVRVVAAEALAHLGEKDKALAALGRVIRTGNLHEILAAQNSAEY
                     LWRAGLITLGDAQELVRGLKFAEPADRIPKLLLSAE"
     gene            complement(4341..5387)
                     /locus_tag="PLP58_18165"
     CDS             complement(4341..5387)
                     /locus_tag="PLP58_18165"
                     /inference="COORDINATES: protein motif:HMM:NF024799.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HRJ10512.1"
                     /translation="MNARILLVLVSCFLSVVTGVVLSRGTGGGGIMEKRARPLIGLSM
                     DTLKEERWAADRDIFTARAAELGADVLVEAANSNDTRQLRDVESLITRKVDALVIIPH
                     NGAAMARAVEMAHAEGIPVLSYDRLITGAEVDLYITFDNLKVGALQARFLAERLPPQG
                     KMRIVRIYGAKTDNNALLFKQGQDQVLQPLIEAGRIEVVHEDWAVDWKPENAKKITNA
                     AITKAGQNIDAILASNDGTAGGAIQALIEEGLAGKVLVTGQDADLAACQRIAHGTQTM
                     TIYKPLHRIASQAAELAVKLALRKPLIAKDGTENGKILVPSVLLDIVAVTKDNLRDTV
                     IKDSFRKEEDVFAK"
     gene            complement(5458..>8300)
                     /locus_tag="PLP58_18170"
     CDS             complement(5458..>8300)
                     /locus_tag="PLP58_18170"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: protein motif:HMM:NF004349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004832 - valine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006438 - valyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HRJ10513.1"
                     /translation="KTYTPAEVEARWYQRWLDDKCFEADPARVSEKRPAYSIVIPPPN
                     VTGILTLGHVLNNTIQDILARRARMLGKEVLWLPGTDHAGIATQNVVEKTLKKQGVIK
                     HRDDLGREGLVAKIWEWKEKHGGIIIEQLKKLGASCDWSRERFTFDDDYNACVMRVFV
                     DLYKKGLIYRGKRMVNWCPSSLTALSDEEVVMKAQNAIMYHFKVEVVEEPGTFLTIAT
                     TRPEVIPGDQAIAVNPKDERYAHLIGKHVRRPLPVENQELLPIIADEHVDFTFGTGVL
                     KVTPAHDKADFEIAQRHNLPAVDVMHPNGVLNDLAGKDLGGMERFAARKRATELLAEI
                     GSLVKEEPYQNNLGYSERADVPIESRLSEQWFLKYPSVKESQAVVAHGEMKFHPDRWA
                     KVYDHWMTGLQDWCISRQVWWGHRIPVWSIVAEGSDTTKTFHVLDAQLQDTLEDLLEG
                     NGENEVLRIIDEQTGELLRPTPVEFNHGLNGKRVIIQIAQMNDNVGRGLEEHGFTQDP
                     DVLDTWFSSWLWPFATMGWPEKTSTLKAFYPTTDLVTGPDIIFFWVARMIMAGFEWMG
                     ELPFKNVYFTGIIRDKQGRKMSKSLGNSPDPLELIASYSADALRFGIMRSAPLGQDIC
                     FDEKNVELGRNFCTKLWNAARFRQMQGGATETEISAALLSSDDKWILLRLNTAIAEVT
                     SALEDYRFSDATATLYRFFWSEYCDWYVEASKAVLQGSDEKRKANTLAVIDFVLGQTL
                     RLFHPFMPFITEELWHGMGFNADLPENQGGKSIMFAPWPKPLDEDELAHFGILPEDEK
                     TANDKYEAVNLGRGLKSTFNINKRVRFVLKPSQELPDHEIEVLRILLNAEPLDVDANY
                     VPTKGTPSALTPLGTIFLPLDGLIDVEAERARVSKEIAKVESELEKVTAKLADTNFTS
                     KVPQKVLDEHQQRKTDWEDKLAKLKVMLEALG"
BASE COUNT         1433 a         2477 c         2738 g         1652 t
ORIGIN      
        1 gcggtgagca cgcgcggcgg ccggccggcg gcggtgaggc gctgggccac ctcggctttg
       61 atggcattca ccaggatgca accgccgacg gtgctgccgg gtgccacggg ggtgtcgaga
      121 ccttcgatgc gcaccatcgc gtcacccacc ggcgcgccgg tgtcgagcac gaggtcggcg
      181 cagtcctgga gctttttccc cgcaggatgg cgggagatgc tggcctcggc gtggtccttg
      241 gaaatgaggg ccacgacttt gacaccgcgc cgctggaact cctccgccat ttccacaggc
      301 acgacattgc agccgctgga ggagacgatg agggcgctgt cgccggcttt gaggtcgaag
      361 ttccgcagga tgcgcgcggc caggccgctg acattttcca ggaacatggc ctggcgctgg
      421 ccgttggcgc cgacgacgag gttgtgaaag gtgaggctga gctcgacgat ggggttgaag
      481 ccggggaagg agccgtaacg cggccacatt tcctccacga ggatgcggct gtgcccggag
      541 ccgaagacat gcaccatctg ccctgcgaga atggtgcggg aaaagaggtc cgccgcctgc
      601 ccgatgagcg ggagctgggc gcgcacgcgc tccaggaggg cggcggagcg ctggaggtag
      661 gattcggcgg gggacatgcg ggttcagtag atgggaaacg cgaatttaga aattcgaaaa
      721 tccggtgaaa gcaggaggcg cggactttga tgacatcttc gggcatgcgc ttccgttaca
      781 aaaaaacttc ccaaccgcca tgacactgaa gcatttgctc cccgccgcgc tctgctgcct
      841 gctcgtcgcc atgcccctgc ccgccgtgga aaagcggccg aattttctct tcatcctcgt
      901 ggatgaccag gcgccctttg acctgaaagt gtatgacccg aagtcgccgc tcgacacgcc
      961 gaacctggac cgcctggccg cccagggggt cgtgtttgat ggcgcgtatc agatgggctc
     1021 cttcagcggc gcggtgtgca tgccctcgcg ccacatgatc atgagcgggc gtacggtctg
     1081 gcatctgccg ccggctccct ggggggcgaa aacctgcccg ccgaacctgg agcagaacag
     1141 tattcccgcc gtcttcaacc gcgccggtta cgccaccatg cgcacctgca agacgggcaa
     1201 cagctatgag gctgcgaaca agcttttcac cgtaaggcat gacgcctcca aacggggtgg
     1261 cgacgacgaa accggcagca cctggcatgg agaccgggtc atggactacc tgcgtgaccg
     1321 ggagtccgcc aaagacgaca agcccttttt cattttctac ggcttctccc acccgcatga
     1381 cacacgcgat ggccggcctg agttgctggc caaatacggc gccgtcaatc acacggacga
     1441 ggccgccccg ccttcgtcgc acagccttca gccggggctg ccgccaaact acctgccggg
     1501 acatcccttt gacatgaccc atgccgacgt gcgcgacgag gtggccgtga gcggtgtttg
     1561 gaagcggcgg gacgaggcca gcatccgcaa cgagatcggg cgccagtacg cctgctctga
     1621 gaacattgac atccagatcg gccgcgtgct gaagcggctg gaggagaccg gcgaactgga
     1681 caacaccttc atcatctaca ccgccgacca tggcatgtcc atcgggcggc acgggctgat
     1741 gggaaagcag aacctctacc agcacacctg gcgcgtgccc ttcatcgtca aaggccccgg
     1801 catcccggcg ggccggcgcg tggagggaaa catttacctg ctcgacatcc tcgccacgtt
     1861 ttgtgatctg gcgggcatcc ccgccccgga gaccaacgag ggcctcagct tcaagccggt
     1921 gctcctgggg gaaaaacccg tcatgcggga cgtgctctac ggcgcctact cgggcggcag
     1981 caagccgggc atccgcacgg tcaagaaagg ggactggaag ctcgtccgct acgagtctcc
     2041 cgaccgctcg gtgaatgaga cgcagctttt caacctggcg gaaaacccgg acgagctcct
     2101 gccgcagcat cacgatccga aagtggccgc cctcaccggg aacatgccaa agccggagca
     2161 aaccaacctc gccagcgatg ccgctcacgc cgccaagctg gcggaaatgc aggcgctgct
     2221 tctggcggag atgcgccgtc tgaacgatcc ctggcgtttt tccgaccagc cggatgacaa
     2281 cctgccagcg cctccagcgc cacagcctcg cggcaagggc aaagggaagg cgggcaaata
     2341 gacgggagag gaggcgtgtc tgttggagcg gccatttccc gcgggttagg cggagccaaa
     2401 acccgcgcca cattgtttcc gcctactccg ccgagagcag cagcttcggg atgcggtcgg
     2461 cgggttcggc gaacttcagt ccgcgcacga gttcctgcgc gtctcccagc gtgatcaatc
     2521 cagcgcgcca gaggtattcg gcgctgttct gcgcggcgag aatttcgtgg aggttgccgg
     2581 tgcggatgac cctgccaagc gcagccagcg ccttgtcttt ttcaccaaga tgcgcgagcg
     2641 cctcggcggc cacgacacgc acgtcgggcc attcgtcttt gagcaagggc agcagcccgc
     2701 ctttggcatg cacagcctgg tcttgaagga tgagacatcc cgtcgcggcc cagtagcgct
     2761 tcaccggatg cggatcggcg agcgcggtga tgaggtcggg cagcttcgag gcatcgcgcg
     2821 cggtggcttt gtccgccagg tccaggatgg ggcccagagg gtaggcgttg ctttgcgcat
     2881 actcgtaaat cgttttgtcg cctgccaggc gcggaaacat gccttccggg atgaagcctg
     2941 tgtcgcgcag ccgcatgatc tcatggcgca atgccgcgcg catggactcg atgcgcgccg
     3001 cctggccggg ctcggcgatc aggttgtgaa tctcatgctt gtccgcgccg gtgtcgtaaa
     3061 gctcctccgg cggtttcggc ttccaatacg cggactgcgc atcattgcag cggcctgcct
     3121 cgaattccgc gaaccaggat ctcatgctgg gctgcacttg gaaggcgtat gtctggtgct
     3181 gcccccaggg acggtgcgga ctgtaattcc gcgcgtaacg aaatccccgg tcgcgcaccg
     3241 cgcgcacggt gtcgtaacgc tcgtccatgc gtcctcggaa aaggaagaca tgctcgcgcg
     3301 gcgcggcttt cttttcgccg aggaagggca cgccctcata atgcgcgggg atgtccacgc
     3361 cgcagaggct gaagacggtg gcgggcaggt ccacaaaagc cacagggtct tccatccagc
     3421 cgccgggttc gacaggacgc cagtttttcc actgttcggg aatgtgaacg atgagcggca
     3481 cgcgggtgcc tgagtcgtgg atgttgcgct tgccgcgcgg cagggcgccg ccgtggtcgc
     3541 cataatagaa aacgatggtg tctttggcct cgcccagctc atcgagttcg gcgagcactt
     3601 tgccggcctc ggcgtccatg agggtcatct ggtcatagta gttggcccag tctttgcgga
     3661 tgtctggagt gtccgggtgg tagggcggca gcggcatctc ctccggcttg atgcggaagc
     3721 tgtccttgcc cttcttcggc gccacctggc tctcgtggct ggtggtgaag ttgaagacgg
     3781 caaagaaggg ctggccgggg cggcggttgc ggtagtgcgc gtttttgctg ctctcatccc
     3841 acacctcgcg cttgccggcg aagttgtagt cctgcttcga gttgttcacg cagtaatagc
     3901 ccgccgcgcg cagatgctcg ggatacatga gaaaatcagc cggatagggc acgctgctgc
     3961 ggtggttgtg gatgcctgtg gagcatgcat gcatgccggt gatgagcgtg gagcgcgccg
     4021 tgctgcacac gggcgcgttt gaaaaagcgt tgcggtagcg gatgccgcgc gcggcgagtt
     4081 tgtcgaggtt tggagtctgg gccagcgcgt cgccgtaaca gccgagatag gggctgttgt
     4141 cctcgctggt gatccacaaa atgttcggac gcgcctgggc gctgagttgg cagagaaaga
     4201 caagcaggag cgcgaaaaga agacggttca tggcggtgtg agggggtttg gtaacgcctc
     4261 catcacccgc ggctttcccg ccatttatgg ggcgctggca tcctgcctgc gcgaacaggc
     4321 gggacgcctg ttccccacga tcacttggcg aacacatctt cctctttgcg gaagctgtcc
     4381 ttgatgaccg tgtcgcgcag gttgtccttg gtgacggcga cgatgtccag cagcacggag
     4441 ggcacgagga ttttgccatt ctcagtcccg tctttggcga tgagcggttt tctcagcgca
     4501 agcttcaccg ccagctccgc cgcctggctg gcgatgcggt gcaggggctt gtaaatggtc
     4561 atggtctgcg tgccatgggc gatgcgctgg caggcggcga gatccgcgtc ctggccggtg
     4621 acgagcacct tgccggcgag tccctcctca atgagcgcct ggatggcgcc tccggcggtg
     4681 ccgtcgttgc tggcaaggat ggcgtctatg ttctgcccgg ccttggtgat ggcggcgttg
     4741 gtgatttttt tggcgttctc cggcttccag tccaccgccc agtcctcatg caccacctcg
     4801 atgcggcccg cctcaatgag cggttgcagc acctggtcct ggccctgctt gaaaagcagc
     4861 gcgttgttgt cggtcttcgc cccgtagatg cggacgatgc gcattttgcc ctgcggcggc
     4921 agacgctccg ccaggaagcg cgcctggagc gcgccgacct tcagattgtc gaacgtgatg
     4981 tagaggtcca cctccgcgcc ggtgatgagg cggtcatagg agaggacggg gatgccctcc
     5041 gcgtgcgcca tctccaccgc gcgcgccatg gccgcgccat tgtgcgggat gatgacgagg
     5101 gcgtccacct tgcgggtgat gaggctttcc acatcgcgga gctggcgggt gtcgttgctg
     5161 ttcgcggcct cgacgagcac atcggcgccc agctcggcgg cgcgggcggt gaagatgtcg
     5221 cggtcggcgg cccagcgctc ctccttgagc gtgtccatgc tcagaccgat gagcgggcgc
     5281 gcgcgttttt ccataattcc gccgccgccc gtgccacggg agaggaccac tcccgtgacc
     5341 acgctgagga agcaggagac caggacaagc aggatgcgcg cgttcatgac ccggcgtttt
     5401 agcggggagc gggcgcgctg accagtggaa aagcgcccgc cctccaccgc aagagactca
     5461 cccgagcgct tcgagcatca ctttgagctt cgcgagcttg tcttcccagt cggttttccg
     5521 ctgctgatgc tcgtcgagca ccttttgcgg gactttggag gtgaagttcg tgtcggcgag
     5581 tttcgcggtg actttttcga gctcagattc gactttggcg atctctttgc tgacgcgggc
     5641 gcgctcggct tccacgtcga tgaggccatc gagcggcagg aagatggtgc ccagcggggt
     5701 cagggcactc ggcgtgcctt tggtcgggac gtagttcgca tcgacatcga gcggctctgc
     5761 gttcagcaag atgcgcagga cttcgatctc gtggtcgggc agctcctggc tcggcttgag
     5821 cacgaaacgg acgcgcttgt tgatgttgaa ggtgctcttc aggccgcggc ccaaattgac
     5881 ggcttcgtat ttgtcgttcg cggtcttttc gtcctcgggg aggatgccga agtgggcgag
     5941 ttcatcttca tccagcggtt tcggccacgg ggcgaacatg atgctcttgc cgccttggtt
     6001 ttcgggaagg tcggcgttga agcccatgcc gtgccacagc tcttcggtga tgaagggcat
     6061 gaagggatgg aacaggcgca gcgtctgccc gagcacgaag tcgatgacgg cgagagtgtt
     6121 ggctttgcgc ttctcgtcgc tgccttggag gacggctttg gaggcctcga cataccagtc
     6181 gcaatactcg ctccagaaga agcggtagag cgtggcggtg gcatcgctga aacggtaatc
     6241 ttccagcgcg gacgtgactt cggcgatggc ggtgttgagg cgcagcagga tccatttgtc
     6301 gtcgctgctg agcaacgcgg cgctgatctc cgtctcggtg gcaccgcctt gcatttgcct
     6361 aaaccgcgcg gcgttccaga gcttggtgca gaagttgcgg ccgagctcga cgttcttttc
     6421 gtcgaagcag atgtcctgac cgagcggcgc gctgcgcatg atgccgaagc gcaatgcatc
     6481 ggcgctgtag ctcgcgatga gctccagcgg atcgggcgaa ttgccgagcg acttggacat
     6541 tttgcggccc tgcttgtcgc ggatgatgcc ggtgaaatag acgttcttga acggcagctc
     6601 gcccatccac tcgaagcccg ccatgatcat gcgggcaacc cagaagaaga tgatgtccgg
     6661 ccccgtgacg agatcggtcg tggggtagaa agccttcaac gtggaggtct tctccggcca
     6721 tcccatcgtc gcaaacggcc acagccagga gctgaaccag gtgtcgagga cgtcggggtc
     6781 ttgggtgaag ccgtgttctt caagtcctct tccgacattg tcgttcattt gtgcgatctg
     6841 gatgataaca cgcttgccat ttaggccatg attgaactcg acaggagttg gtcgaagcag
     6901 ctcgccggtt tgctcgtcga ttatgcgaag cacttcattc tcgccattgc cttcaagcaa
     6961 gtcttccagt gtatcctgaa gctgtgcatc aagaacatgg aaagtcttcg ttgtgtccga
     7021 accttcggca acaatgctcc acaccggaat ccgatgcccc caccagacct ggcggctgat
     7081 gcaccagtct tggaggccgg tcatccagtg gtcatagact ttcgcccagc ggtcggggtg
     7141 gaatttcatc tcgccatggg ccacgacggc ctggctttcc ttcacgctcg ggtatttgag
     7201 gaaccactgc tcgctcaaac ggctctcgat gggcacgtcg gcacgctcgg agtagccgag
     7261 gttgttttga tatggctcct ccttcacgag cgaaccgatt tccgcgagca gttcggtggc
     7321 gcgtttgcgc gcggcgaaac gctccatgcc gccgaggtct ttgccagcga ggtcgttgag
     7381 cacgccattc ggatgcatga catcgaccgc aggcaggttg tgacgctgcg cgatctcaaa
     7441 gtcggccttg tcatgcgccg gggtgacttt cagcacgccg gtgccgaagg tgaaatcgac
     7501 atgctcatcg gcgatgatcg ggagcagctc ttgattttcg accggcagcg gacggcgcac
     7561 gtgtttgccg atgagatgcg cataacgctc gtccttcgga ttcacggcga tggcctggtc
     7621 tcccgggatg acctcggggc gcgtggtggc gatggtgagg aaggtgccgg gttcctcgac
     7681 gacctcgact ttgaagtgat acatgatggc gttttgcgcc ttcatgacga cctcctcatc
     7741 gctgagcgcg gtgagggagg acgggcacca gttcaccatg cgcttgccac ggtagatgag
     7801 gccctttttg tagagatcga cgaacacgcg catgacgcag gcgttgtagt cgtcgtcaaa
     7861 ggtgaagcgc tcgcggctcc agtcgcagga ggcgccgagt ttcttgagct gctcgatgat
     7921 gatgccgccg tgcttctcct tccactccca gatctttgcc acgaggccct cgcggcccag
     7981 atcgtcgcgg tgcttaatga cgccctgctt tttcagcgtc ttctcgacga cgttttgcgt
     8041 ggcgatgccg gcgtggtcgg tgccgggcag ccagagcact tccttcccga gcatgcgggc
     8101 gcggcgggcg aggatgtcct ggatggtgtt gttcagcaca tggccgagcg tgaggatgcc
     8161 cgtgacgttc ggcggcggga tgacgatgga atacgccggg cgcttctcgc tgacgcgggc
     8221 gggatcggct tcaaagcact tgtcgtcgag ccagcgctga taccagcggg cttcgacctc
     8281 ggctggtgtg taggttttgt
//
LOCUS       DAOMYJ010000225         8297 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Prosthecobacter sp. isolate AMR_MDS_5629
            NODE_2870_length_8297_cov_21.159063, whole genome shotgun sequence.
ACCESSION   DAOMYJ010000225 DAOMYJ010000000
VERSION     DAOMYJ010000225.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867850
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prosthecobacter sp. (wastewater metagenome)
  ORGANISM  Prosthecobacter sp.
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Verrucomicrobiaceae; Prosthecobacter.
REFERENCE   1  (bases 1 to 8297)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8297)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.30
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:34:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,742
            CDSs (total)                      :: 4,684
            Genes (coding)                    :: 4,676
            CDSs (with protein)               :: 4,676
            Genes (RNA)                       :: 58
            tRNAs                             :: 55
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8297
                     /organism="Prosthecobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2870_length_8297_cov_21.159063"
                     /isolate="AMR_MDS_5629"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1965333"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..358
                     /locus_tag="PLP58_18175"
     CDS             <1..358
                     /locus_tag="PLP58_18175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ10514.1"
                     /translation="LLALTGCAFGCLCLGISACSADAGRAHSRAWMLFFFNLILAGAL
                     LGFPRVLGGVIHPFITAYHGWSGSVETLRDTAVFEPVTRLVRTWFATPAQAMWALGGH
                     SLVGVLLAWFGLRNRR"
     gene            393..1358
                     /locus_tag="PLP58_18180"
     CDS             393..1358
                     /locus_tag="PLP58_18180"
                     /inference="COORDINATES: protein motif:HMM:NF015692.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribosylglycohydrolase family protein"
                     /protein_id="HRJ10515.1"
                     /translation="MPPSRQQRILGGLFGSLVGDALGVPVEFQPRARRKGDPVTSMRG
                     FGTHNQPAGTWSDDGSLLLCAAESLAEHGFDTQDMGRRFARWLDEGRWTAHGLVFDIG
                     NATRQALDRIALGTPAEEAGGRSESSNGNGSLMRILPVPLASLDCGLDNFCERVARAS
                     AITHAHERAQLACVLHGLLARALMRGEIPAAAHASAAAEFKARFASRTEFPHFSTLLD
                     PGLASQRGDAIQSSGYVLHTLAASIWCLLTTGTFSECVLKAVNLGDDTDTTGCVAGGL
                     AGVFYGIESIPADWIGALPRQEALRGLFERFVSAAAAHPPHCQDA"
     gene            complement(1425..2189)
                     /locus_tag="PLP58_18185"
     CDS             complement(1425..2189)
                     /locus_tag="PLP58_18185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007413685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRJ10516.1"
                     /translation="MTLTEENAALHEGREPIIRLVDIRKSYQMGDVVSQVLQGISFDI
                     YPGEYVCIMGPSGCGKSTLLNVLGCLDQPTSGDYFLGGENVATLNDDDLSAARNRNLG
                     FIFQSYNLIQQLTVLENIAVPMYYGGADDALMRRTAEKLARRVGLEHRMNHKPNELSG
                     GQQQRVAIARALSNSPLMILADEATGNLDSKSGQEILALFDELNAEGKTLVFVTHDER
                     MVQRCTRIIRLRDGLLESDQPGAKARARPAPRLAAA"
     gene            complement(2186..4009)
                     /locus_tag="PLP58_18190"
     CDS             complement(2186..4009)
                     /locus_tag="PLP58_18190"
                     /inference="COORDINATES: protein motif:HMM:NF024829.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HRJ10517.1"
                     /translation="MMKLKAWHLVVGAAVIAVAAWFMLRGPGGAADDNVPTFAVQKGP
                     LQINVLQGGEIRALRNFEHKSEIETPTKIISVIPEGYLVTEEDVKEGKVLIELDSTDL
                     RTRIQDHEIQFQTTVSMYIDADEGREIQRSENQSLVRDMKQTAGFALMDFEKYLGRQL
                     AADILVAAGLPKDIEAFDRFADDLEQQANVQLEAGAALKVAQQVKETPGPAVEPQNTD
                     AVPLHADRMDFSPYLERQDSGDGEAQQKLRQLEDELLLRKSELAVAKQKVEASQRLAA
                     REFISRTQLENDQVNFEKVSLSVKTAETQLSLFKTYEFSKQCALLLSEYRESLTKLQR
                     TVRANRSKMAQAETRFQTAKRRYDMELAKKDDLDRQLKACLIRASQPGLVAYGNLNAS
                     SSTRYSEPIEEGATIRFRQTLLTIPDMSQMGVSVNIHESQVKKVRIGQPALIRVDAEP
                     GKVLEGRVAELALMPDSGSSRYTPNLKVYPASVHIFGTHTWMKPGMNAKVEVIVDQLA
                     DVMFVPVQSIEVQDDKHYCYVTEGSGIARRSVVTGLFNDEFIEVREGLAFGEKVALSI
                     PKSLTSEPKPATGPAQQPSGAPQERKPKETPKARDVAAVRG"
     gene            complement(4006..5697)
                     /locus_tag="PLP58_18195"
     CDS             complement(4006..5697)
                     /locus_tag="PLP58_18195"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HRJ10518.1"
                     /translation="MPRSPLAALLPAALLPGLCLLSGCTQSFYKRWADREVFGIIGNK
                     SRVVAGTDETLLDITPPPPAKLDALEKSRNNSDFLGERAFIEKNARMLGLADALDFAV
                     HRNRGYLGRKETVYLSALRLTLTRQQFGPVADGGGGVFYTERQVKNGVNDFVRTSTLT
                     TDGIVGFDYLMRTGARLAMDLSTDFTRFFTGATPKLSDSRAAATLSQPLLRGAGVLAA
                     SEPLRQDEREVLYAIRDFTQFRKQFTVDTARQYLRVLQAREAARNRDTAYRAAILSLE
                     RERALADANLRSQSSLRQIEQGLLSYERSRITAIRNYEDQLDDLKIALGLPVTERIVL
                     DNKEIENLQVIDPSGTLDEAQDTALITRLDLFNERDRLQDAARKVKIAHQNTLPGVNA
                     LVNYEIGTPGGNEGLELNHRQRRLTAGLDVDLNLNTKPERNVLRSSQITEQRSARELE
                     LAEENLRGNIRADWRALEVARQQYDLAVEGLALAEKRLEIEEALMAEGQGTARDIVES
                     QDRLIAARDLVVSSKIDHVIARLQLWSDMGILFIQKDGRWADVLKNEKNQGTEEE"
     gene            5842..7686
                     /locus_tag="PLP58_18200"
     CDS             5842..7686
                     /locus_tag="PLP58_18200"
                     /inference="COORDINATES: protein motif:HMM:NF020976.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase D family protein"
                     /protein_id="HRJ10519.1"
                     /translation="MQAARHILSLILVPALAAQVPMEEYSTNHVTHGPMLGLVTDSSV
                     RVWARTHRAGEFTVRYGENAGSLEQGSAPVRTSAERDHTGSVTLTGLKPDTLYHYQVF
                     IGKVPSGPGGSFLTLPGAEAHREAAHNPKGLFNFRFQFGSCANQNPKSGIGPSLPAHA
                     TMLRELHGRTYFSIMNGDFIYEEHRSMSPEAWLMQAGLMPDQAPRIVDLAPNIVGLWE
                     NYKTYLSRGVNLAAWQRNMPTVFTFDDHELVNDIRGCGTTGFRERRAVFRDIGIRAWE
                     DYAGWANPYETTQGIHFGRAILKKGSDILVDEQADFTKLDMTQAANLHVHWGKITAGE
                     DDIRLDKEPPGNPNAGVFAIAEVLDAHRLRITPAAYADSTGSYSIGRKSYGKFTVSNC
                     DYFIIDSKTHREMHDIKDPTRAGLSFLGKDQKAWLLDEMRRSTSDFFFIVSSVNFMIP
                     HNGAGGHEFTEGKDEAWTALIAEREELIEFFDALKKPVFILTADLHNSFAIKITDRVW
                     EFSSGPLNSVNHVPVNDEGNRPATGIYQSGPRKCDIRWSSYILPDLERPQRMYPYYCV
                     VQVNNVYNMPQQLGDTRWVAYPHPQVIFQYFDGRTGEHVYSEAISTAR"
     gene            7683..>8297
                     /locus_tag="PLP58_18205"
     CDS             7683..>8297
                     /locus_tag="PLP58_18205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ10520.1"
                     /translation="MKKARIKKGCAAALLLPVLAITGLLIMAGCANRVPHLPVPPGIT
                     KRTDKLTLQGSEVPVDIYLPQKADKAPVVIVAHGFTRNRRVMAGWGILLAQNGMIAAV
                     PDLPAFADHGRNARAIGDLITLLYEPGRPGQAAPTGDVALIGHSAGGFATLLAAGREK
                     RARCWVGLDPVDFMGHGLAAVKKLKVPALMLLAESGAWNRHANAL"
BASE COUNT         1323 a         2695 c         2709 g         1570 t
ORIGIN      
        1 gctgctggcc ctcacgggct gcgccttcgg ctgcctctgc ctcggcatct ccgcgtgcag
       61 cgccgacgcc gggcgcgcgc acagccgcgc ctggatgctg ttctttttca acctcatcct
      121 cgccggggcg ctgctgggct tcccgcgcgt gctcggcggc gtcatccacc ctttcatcac
      181 cgcctaccac ggctggtcgg gcagcgtcga gactctgcgt gacaccgccg tcttcgagcc
      241 ggtgacgcgc ctggtccgca cctggttcgc cacgcccgcc caggccatgt gggcgctcgg
      301 cgggcactcg ctcgtcggag tgctgctcgc ctggttcggg ctgcggaaca ggcggtgaaa
      361 cgacggattt caccagctcc ggaggaactc tcatgccgcc ttcccgccag caacgcatcc
      421 tcggcggtct tttcggctcg ctggttggcg acgctctcgg ggtgccggtg gagtttcaac
      481 cgcgcgcgcg gcggaagggc gatcctgtca cgtccatgcg cggctttggc acgcacaacc
      541 agcccgcggg aacctggtca gatgacggct cgctgctgct ctgcgccgct gaaagtctgg
      601 cggagcatgg attcgacacg caggacatgg ggcggaggtt tgcgcgctgg cttgatgaag
      661 gccgctggac tgctcacggc ctcgtcttcg acattggcaa cgccacccgg caagcgttgg
      721 accggatcgc gctcggcact ccggcggagg aggccggtgg ccggagcgag tccagcaacg
      781 gcaacggctc gctcatgcgc atccttcccg tgccgctggc ttccctggac tgcggcctgg
      841 acaacttctg cgagcgcgtc gcccgcgcct ccgcgatcac gcacgcgcat gagcgcgccc
      901 aactggcctg tgtgctgcac gggttgctcg cgcgcgccct gatgcgcggt gaaatccccg
      961 ccgctgccca tgccagcgcc gcggctgagt tcaaagcgcg ctttgcgtcc cgcaccgagt
     1021 tcccgcattt ctccacgctg ctggatcccg gcctcgcctc gcaacgcggg gatgccattc
     1081 aatccagcgg ctacgtcctg cacacgctcg ccgcctcgat ctggtgcctg ctgaccacgg
     1141 gcaccttttc cgagtgcgtg ctcaaagccg tgaacctcgg cgacgacacc gacaccacag
     1201 gctgcgttgc cggaggtctc gcgggcgtgt tttatggcat cgaatccatc cctgcggact
     1261 ggatcggcgc cctgccgcgg caggaggcgc tgcggggcct tttcgagcgc ttcgtgtccg
     1321 ctgcggctgc tcatccaccg cactgccagg acgcatgagc gcgctttcat caccggctca
     1381 catgccggag ctttccgaag ggtgaatggt gatttgaagc caattcacgc ggcggcgagg
     1441 cgcggggcgg ggcgcgcgcg ggctttggcg ccgggctggt cgctttcgag gaggccgtcg
     1501 cggaggcgga tgatgcgggt gcagcgctgg accatgcgct cgtcgtgggt gacgaagacg
     1561 agggtcttgc cttcggcgtt gagctcgtca aaaagcgcga ggatttcctg gccggacttg
     1621 gagtcgagat tgcccgtggc ctcgtcggcg agaatcatga gggggctgtt ggagagggcg
     1681 cgggcgatgg cgacgcgctg ctgctggccg ccggagagct cgttgggctt gtggttcatg
     1741 cggtgctcca ggccgacacg gcgcgcgagc ttctcggctg tcctcctcat gagcgcgtca
     1801 tccgcgccgc cgtaatacat gggcacggcg atgttctcca ggacggtgag ctgctggatg
     1861 aggttgtagc tctggaagat gaaacccagg ttgcggttgc gggcggcgga gaggtcgtcg
     1921 tcgttgagcg tggcgacgtt ttcgccgccg aggaagtagt ccccgctggt gggctggtcg
     1981 aggcagccta ggacgttgag cagggtggac ttgccgcagc cggaggggcc catgatgcag
     2041 acgtattccc ccggatagat gtcgaacgag atgccctgga gcacctggct gacgacatcc
     2101 cccatctggt aggatttgcg gatgtccaca agccggatga tgggctcccg cccttcatga
     2161 agggcggcgt tttcctccgt cagtgtcatc cgcgcacggc ggccacgtcc ctggccttgg
     2221 gggtttcctt cggcttgcgc tcctgcgggg cgccggaggg ctgctgggcc gggccggtgg
     2281 cgggtttggg ctccgaggtc aggcttttgg ggatggaaag ggcgaccttt tctccgaagg
     2341 ccaggccttc cctgacttcg ataaactcgt cgttgaacaa acctgtgacg acactgcgac
     2401 gggcgatgcc cgagccttcg gtgacgtagc agtagtgctt gtcgtcctgg acttcgatgg
     2461 actgcacagg cacgaacatg acatcggcga gctggtccac gatgacctcg actttggcgt
     2521 tcatgccggg cttcatccag gtgtgcgtgc cgaagatgtg gacggacgcg ggatagactt
     2581 tgaggttggg cgtgtagcgg ctgctgcccg agtcgggcat cagggccagc tcggcgacgc
     2641 gtccttcgag caccttgccg ggctcggcat ccacgcggat gagggcgggc tggccgatgc
     2701 ggactttttt gacctgggat tcgtggatgt tcacgctgac gcccatctgc gacatgtccg
     2761 ggatggtgag cagagtctgg cggaagcgga tggtggcgcc ctcctcgatg ggctcgctgt
     2821 agcgtgtgga gctgctggcg ttgaggttgc cataggcgac gagacccggc tgggaggcgc
     2881 ggatgaggca ggctttgagc tggcggtcca ggtcgtcttt cttggcgagc tccatgtcat
     2941 agcggcgctt ggcggtctgg aagcgggtct ccgcctgggc catcttggag cggttggcgc
     3001 gcacggtgcg ctggagcttg gtcagactct cacggtattc ggacaggagc agggcgcact
     3061 gcttggaaaa ctcgtaggtt ttgaacaggc tgagctgggt ttccgccgtc ttgacggaaa
     3121 gggagacttt ttcgaagttg acttggtcgt tctccaactg ggttcgggag atgaactcgc
     3181 gcgcggcgag gcgctgggaa gcttcaactt tctgtttggc gaccgcgagc tccgatttgc
     3241 gcaggaggag ttcgtcctca agctgcctga gcttttgctg ggcctcgccg tcaccgctgt
     3301 cctgcctctc aagatagggc gagaaatcca tgcgatccgc gtggaggggc acggcgtccg
     3361 tgttttgcgg ttcaacggct ggccccgggg tttccttgac ttgctgtgcc accttcaagg
     3421 cggcgcccgc ttcaagctgg acgttggcct gctgttcaag gtcgtcggcg aaacggtcga
     3481 aggcttcgat gtccttggga aggccggcgg cgacgaggat gtcggcggca agctggcggc
     3541 cgaggtattt ttcaaagtcc atcagggcaa agccggcggt ctgcttcatg tcccgcacga
     3601 ggctctgatt ctcactgcgc tggatttcgc gcccttcgtc cgcgtcaatg tacatggaga
     3661 ccgtggtttg gaactggatt tcgtggtcct gaatgcgggt gcgcaaatcg gtgctgtcca
     3721 gttcgatgag caccttgccc tccttcacat cctcctcggt caccaggtat ccctcgggaa
     3781 tcacactgat gatcttggtg ggcgtctcga tctccgactt gtgctcgaag ttcctcagcg
     3841 cgcggatttc accaccttgc agcacgttga tttggagcgg gcctttctgc acggcgaagg
     3901 tgggcacgtt gtcatcagcc gcgccgccag ggccgcgcag catgaaccag gccgcgaccg
     3961 caatgaccgc tgctccgacc accagatgcc atgctttcaa tttcatcatt cttcctcggt
     4021 tccctgattc ttttcgtttt tcaatacgtc cgcccaccgg ccgtctttct gaatgaatag
     4081 aattcccatg tcgctccata attgcaggcg ggcgatcaca tggtcaattt tcgacgacac
     4141 cacaaggtcg cgcgcggcaa tgagccggtc ctgtgattcc acgatgtcac gcgcggtgcc
     4201 ctggccttcg gccatgagcg cctcctcgat ctccagcctc ttctcggcca gcgccaggcc
     4261 ttccacggcc aggtcgtact gctgccgcgc cacctccagg gcgcgccagt ccgcgcggat
     4321 gttgccgcgc aggttttcct ccgccagctc cagctcgcgc gcggagcgct gctcggtgat
     4381 ctggctggag cgcagcacat tgcgctcggg tttggtgttc aggttcaggt ccacatcaag
     4441 gccggctgtc aggcggcgct ggcggtggtt gagttccagg ccctcgttgc cgccgggcgt
     4501 gccgatctcg taattcacca gcgcgttgac gccgggcagt gtgttctggt gggcgatttt
     4561 caccttgcgc gccgcgtcct ggaggcggtc gcgctcattg aagaggtcta gccgcgtgat
     4621 cagggcggtg tcctgcgcct cgtccagcgt gccggacggg tcaatgacct ggaggttttc
     4681 gatctccttg ttgtccagca cgatgcgctc ggtcaccggc aggccgaggg cgatcttcag
     4741 gtcgtcgagc tggtcctcgt aattgcggat ggcggtgatc ctggagcgct cataggagag
     4801 cagcccctgc tcgatctgcc gcaggctgga ttgagagcgc aggttcgcgt ccgcaagcgc
     4861 gcgctcgcgc tccagggaaa ggatggcggc gcggtaggcc gtgtcgcggt tccgggccgc
     4921 ctcgcgcgcc tgcagcacgc gcaggtattg gcgcgccgtg tccacggtga actgtttgcg
     4981 gaactgggtg aaatcccgga tcgcgtagag cacctcccgc tcgtcctggc gcaggggctc
     5041 cgaggccgcc agcacgcccg cgccgcgcag cagcggctgg gagagcgtgg ccgccgcgcg
     5101 ggaatcgctc agcttcggcg tggcgccggt gaaaaagcgg gtgaaatccg tgctcaggtc
     5161 catcgccagg cgcgcgcccg tccgcatgag atagtcgaag cccacgatgc cgtcggttgt
     5221 cagcgtcgag gtccggacaa agtcgttcac cccgttcttc acctgccgtt ccgtgtaaaa
     5281 caccccgccg ccgccgtcgg ccaccggccc gaactgctgc cgcgtcaaag tcagccgcag
     5341 cgcgctgagg tacactgtct ccttgcgccc cagatagccg cggttgcgat gcacggcgaa
     5401 gtccagcgcg tcggccaggc ccagcatccg ggcgtttttt tcgatgaagg cgcgctcgcc
     5461 gaggaagtcg ctgttgttgc ggcttttctc cagcgcgtcc agtttcgcgg ggggcggggg
     5521 agtgatgtcc agcagcgtct catcggttcc cgccaccacc cgcgacttgt ttccgatgat
     5581 cccgaagacc tccctgtccg cccagcgctt gtagaaagac tgggtgcagc cggagaggag
     5641 gcacaggccg ggcaaaagcg cggctgggag gagggcggct agggggcttc taggcatgtc
     5701 ggatgtgtgc gcgagatcac cctccggggc gctcgaatga ggggggagtt tttcaaagcg
     5761 gcccggcggg tccaatcaac gcccgcagct cgcggcttct atcagcaagg ccgtgttcgg
     5821 agccgcgttg tgacatgcct catgcaagcc gcgcgtcata ttctcagcct catcctcgtc
     5881 cccgccctgg ccgcccaggt tccgatggag gaatacagca ccaatcacgt cacccacggc
     5941 cccatgctcg gcctcgtcac cgactccagc gtgcgcgtct gggcgcgcac gcaccgggcg
     6001 ggggagttca cggtgcgcta cggtgagaat gccgggagcc tggagcaagg ctcggctccg
     6061 gtgaggacga gcgcggagcg cgaccacacc ggctccgtca cgctcaccgg gctgaagccg
     6121 gacacgcttt accattacca ggtcttcatc ggcaaggtgc cctccggccc cggcggcagc
     6181 ttcctcaccc tgcccggcgc ggaggcgcat cgcgaggccg cgcacaatcc caagggcctg
     6241 ttcaacttcc gcttccagtt cggctcctgc gccaatcaga atccgaagag cggcatcggc
     6301 cccagcctgc cagcgcacgc caccatgctg cgggagctgc atggccggac gtatttttcc
     6361 atcatgaacg gcgacttcat ctatgaggag caccgctcca tgtcgcccga ggcctggctc
     6421 atgcaggccg ggctgatgcc ggaccaggcg ccgcgcatcg tggacctcgc gccgaacatc
     6481 gtcggcctgt gggagaacta caaaacctac ctctcacgcg gcgtgaacct ggccgcctgg
     6541 cagcggaaca tgcccaccgt cttcaccttc gacgaccacg aactggtcaa tgacatccgc
     6601 ggctgcggca ccaccggctt ccgcgagcgc cgcgccgtct ttcgcgacat cggcatccgc
     6661 gcctgggagg actacgccgg ctgggccaac ccgtatgaaa ccacgcaggg catccatttt
     6721 ggccgcgcca ttctgaaaaa aggcagcgac atcctcgtgg acgagcaggc cgacttcacc
     6781 aagctcgaca tgacccaggc cgccaacctg cacgtccact ggggcaagat caccgccggc
     6841 gaggatgaca tccgcctcga caaagaaccg cccggcaacc ccaacgccgg cgtctttgcc
     6901 atcgccgaag tgctcgatgc gcaccgcctg cgcatcacgc ccgccgctta cgcggacagc
     6961 accggctcct attccatcgg gcgcaagtca tacggcaaat tcaccgtgtc gaactgcgac
     7021 tacttcatca ttgattccaa gacgcaccgc gagatgcacg acatcaagga cccgacacgc
     7081 gcgggcctca gcttcctcgg caaagaccaa aaggcctggc tgctcgacga gatgcgccgc
     7141 agcaccagcg acttcttttt catcgtctcc tccgtcaatt tcatgatccc gcacaatggc
     7201 gcaggcgggc atgagttcac cgagggcaag gacgaggcct ggaccgcgct catcgccgag
     7261 cgcgaggagc tgatcgagtt cttcgacgcg ctgaaaaagc ccgtcttcat cctcacggcg
     7321 gacctgcaca acagcttcgc catcaagatc accgaccgcg tgtgggagtt ctcctccggc
     7381 ccgctgaact ccgtgaacca tgtgcccgtc aacgacgaag gcaaccgccc cgccaccggc
     7441 atctaccagt ccggcccgcg caagtgtgac atccgctgga gcagctacat cctgccggat
     7501 ctggagcgcc cgcagcgcat gtatccctat tactgcgtcg tgcaggtgaa caacgtgtat
     7561 aacatgccgc agcagctcgg tgatacacgt tgggtggcct acccgcaccc gcaggtcatc
     7621 ttccaatact tcgacggccg caccggcgag catgtgtact ccgaggccat cagcaccgcg
     7681 cgatgaagaa agcacggatc aaaaaaggct gcgccgccgc gctcctgctg cccgtgctcg
     7741 ccatcacggg cctgctcatc atggccgggt gcgcgaaccg cgtgccacac ctgcccgtgc
     7801 cgcccggcat cacgaaacgg acggataaac tcacgctcca gggcagtgag gtgccggtgg
     7861 acatttacct gccgcaaaaa gcggacaaag cgccggtggt catcgtcgcg catggcttca
     7921 cacgaaaccg ccgcgtcatg gccgggtggg gcatcctgct ggcgcaaaac ggcatgatcg
     7981 ccgccgtgcc agacctgccc gcctttgccg accatggccg caacgcgcgc gccatcggtg
     8041 atttgatcac gctgctgtac gagccgggca ggcccggtca agcggcgccc acgggagatg
     8101 tcgcgctcat cgggcattcg gcgggcggct ttgccacgct gctggcggcg ggccgggaaa
     8161 aacgcgcgcg ctgctgggtc gggctggatc cggtggactt catgggccac ggccttgcgg
     8221 cggtgaaaaa gctgaaagtc cccgcgctca tgctcctggc cgagtccggc gcgtggaacc
     8281 gccatgccaa cgcgctg
//
LOCUS       DAOMYJ010000226         8237 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Prosthecobacter sp. isolate AMR_MDS_5629
            NODE_2901_length_8237_cov_17.919702, whole genome shotgun sequence.
ACCESSION   DAOMYJ010000226 DAOMYJ010000000
VERSION     DAOMYJ010000226.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867850
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prosthecobacter sp. (wastewater metagenome)
  ORGANISM  Prosthecobacter sp.
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Verrucomicrobiaceae; Prosthecobacter.
REFERENCE   1  (bases 1 to 8237)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8237)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.30
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:34:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,742
            CDSs (total)                      :: 4,684
            Genes (coding)                    :: 4,676
            CDSs (with protein)               :: 4,676
            Genes (RNA)                       :: 58
            tRNAs                             :: 55
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8237
                     /organism="Prosthecobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2901_length_8237_cov_17.919702"
                     /isolate="AMR_MDS_5629"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1965333"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..489
                     /locus_tag="PLP58_18210"
     CDS             <1..489
                     /locus_tag="PLP58_18210"
                     /inference="COORDINATES: protein motif:HMM:NF024829.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family efflux transporter periplasmic
                     adaptor subunit"
                     /protein_id="HRJ10521.1"
                     /translation="LRAPQDGVVLTPDLDRLAAGSLRAGDTLCTLAAEGQAEVFFPLS
                     QRQARHVRAGQKMELRAASRPGVTFTGTVAEDAKTPPAESLPPNLAATLGGDIAAQPD
                     DKGGLKPLEVTYGVLVRLPDPDGLLRPGMTGTGRLHGDRVTLGAALWMRLLDFISLDY
                     RW"
     gene            complement(505..2016)
                     /locus_tag="PLP58_18215"
     CDS             complement(505..2016)
                     /locus_tag="PLP58_18215"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase"
                     /protein_id="HRJ10522.1"
                     /translation="MRHLLPLTLLTFLPLLSSARAPNILWFVVDDMSPHFSCYGETAI
                     QTPAVDRLAAEGARFTRAYVTAPVCSACRSAMITGMYQTTIGAHHHRSGRGELKIHLT
                     EGVRPVPELFQKAGYFTCIGSGLEERDFRGLPFGEGRAGKKKAAGAPMKGRLGKTDYN
                     FEWDTAMYDSHDWAGCGEKPFFMQVQLHGGKLRADNLEANRALAARARSELGSATDPQ
                     KVTLPPYYPRDPVLLEDWAAYLDSVRLTDLHVGRVIARLEKEGLLENTLVIFMTDHGI
                     SHARGKQFLYDEGAHIPFIVRGPGIAKGAVRTDLVEHIDMAALSLAAAGIPIPEAMQG
                     RDIFAKDYQPRQAVFAARDRCDETVERIRSARTERFLYIRNFLPRRPHLQPNAYKDKK
                     AIVAALRTAHEAGTLPSVSEELLFSPERAIEELYEYVNDHWQVTNLAANPDYKTELEA
                     HRARLDEWIARTGDKGPESEAMYDSDMAEYLKKSNPDVERNIVLMKQWAREGK"
     gene            2015..2842
                     /locus_tag="PLP58_18220"
     CDS             2015..2842
                     /locus_tag="PLP58_18220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012420795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HRJ10523.1"
                     /translation="MAGARRRNDRVTNGPGGDFLHLIRRQGDKAFARPAPVLQHRRMS
                     SIAERLENIRERIAAAARHAGRAAGEVELVAVSKTFPAEAVREAAEAGQTLFGENRVQ
                     ELLAKQPVLPSRLRWHLIGPLQSNKVRKVLPAADMLEAVHSLEIARDIQRVSEELGLR
                     PRVLLEINVAAESSKHGFSPDQARARLEELYGLDRLQIEGLMCIPPYDSGPEKSRRHF
                     AALRALRGELEKAGGAPLPQLSMGMSHDFEAAVEEGATLVRIGSAIFGSRPPARMPL"
     gene            2918..3907
                     /locus_tag="PLP58_18225"
     CDS             2918..3907
                     /locus_tag="PLP58_18225"
                     /inference="COORDINATES: protein motif:HMM:NF021507.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphodiester glycosidase family protein"
                     /protein_id="HRJ10524.1"
                     /translation="MRVLLLLLLAVFLAAWVAAQWVFNIHSNLVAVCVRDSRESLLVR
                     VRGDTWKTPAQITPAEMAAALVERHREPGLVWSTVRVRRPAGLAGAVAQKVFGAEVHV
                     VTISPKEFEFMASYKPGFEVTTASERLATEKLWFAVTANFRDPKGRPLGWVWHEGRET
                     NRAFPEWSGCFFVKNGVPFFGPKSLLDEVPGAIEEGVQVYPSVMKNHTIFSYVGLKPD
                     QYFDGSKISYRSLGGMRRDGAIVFVLSGSGGVMNVMETAEIARKLDIQHATLLDGGRA
                     LQYSLRTEDGPWHFTSFNTRVKFKPGRFEQQLSPVFIGVRRHAPEIIAVPPES"
     gene            complement(3902..4816)
                     /locus_tag="PLP58_18230"
     CDS             complement(3902..4816)
                     /locus_tag="PLP58_18230"
                     /inference="COORDINATES: protein motif:HMM:TIGR01777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01777 family oxidoreductase"
                     /protein_id="HRJ10525.1"
                     /translation="MRIGITGATGFIGRHLTALARESGEVIAYTRGHAALQLADRTLR
                     LPGDAPHALPETRLDALVHLAGEPLAGLWTREKRDRIWRSRVDFTESLVAHLGTWKME
                     NRPRVLVCASGAGFYGDSGGVVVDEAAPQGAGFLAEICGRWEAAAQGAEAAGMRVVTL
                     RTGMVLGPDGGAYPLLRRVFRLGLGGRLGSGAQWMSWIHIRDAARMILRAVEDESWRG
                     PLNVCAPGAVTNAQFTRALAAAVRRPAFFHAPAFGLRLLLRGMADEMLLGGQRAAPAK
                     ALEAGFRFDFPRVDDAMADLEGRRTCGS"
     gene            complement(4856..5164)
                     /locus_tag="PLP58_18235"
     CDS             complement(4856..5164)
                     /locus_tag="PLP58_18235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ10526.1"
                     /translation="MSVPLTAFLVHLRQPAGGAHTADTAALDWFVIATLCCFFFLLGC
                     FAAVAWGIWRRTTRPEPHEKLLQELAEADEDEILRAGESGPDASRAPWERQADWWRRE
                     "
     gene            complement(5168..5641)
                     /locus_tag="PLP58_18240"
     CDS             complement(5168..5641)
                     /locus_tag="PLP58_18240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009959821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HRJ10527.1"
                     /translation="MPAISTEPRIEPATIEDMPQLVELLMALFSEEEDFRPDRTKQEH
                     GLRLILEQPNRGRIFVLRTDYQVIGMVNLLFTISTAEGGLVILMEDVIVHPQHRRQGY
                     GARLLDHAIEFAREKNFRRITLLTDKISAESQAFFAKHGFSHSSMIPMRLVFASS"
     gene            complement(5766..7148)
                     /locus_tag="PLP58_18245"
     CDS             complement(5766..7148)
                     /locus_tag="PLP58_18245"
                     /inference="COORDINATES: protein motif:HMM:NF013672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HRJ10528.1"
                     /translation="MVVYLAVMWIRFFLLFSFHASLALAQTSDAGGVKVSLQRYSGPA
                     TVSVGSLDKFKLRVQLERNGETQPLNVRVELPKSGRQVWPANDVEVRDEAGEALLVQR
                     SGIEWENIVVPVAGKLNAFMVQAVPPAVPAPQTTAEKDRVIQDEVSGAEVRIANWPQG
                     RTAALSIRFDDSHPTHLSKAIPILGEYGFKGTFMVNPGPKEAGSRQNYAFETQLAEWA
                     AVAERGDMELANHSAHHRGAKGDEDMDREIGSAAEAIWKLTPGKSKLLALNLGGGTQW
                     QTTRTLRHYLDKYHQFDASSGSLGMDDSYGGRVEAFRKALATHLERGLWCRVHYHYIG
                     EGLSSSEANFRAALDIAKAHQEQLWIAGMADIHKYQTERSSAKITLLKSDAKSLAFQL
                     DCTTDATLYDQPLTIEITPPAAWDAAKIAVKDETGAALPVQLGESGGRRLPRLEVPAR
                     KGVYSVSTTP"
     gene            complement(7183..>8237)
                     /locus_tag="PLP58_18250"
     CDS             complement(7183..>8237)
                     /locus_tag="PLP58_18250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009965421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HRJ10529.1"
                     /translation="DISPTGKARKIFTLREPLSLVAAITPFNHPLNQVAHKLAPAIAA
                     GAPVILKPSEKTPLTAVRLVELLYEAGLPGHLLSCIHGNLDEVTRPMIRDERIDLVTF
                     TGSAKVGKEIAATAGYKKTCLELGGHSPLIVLDDADLDLAAKLACEGCFRNSGQRCTA
                     VRRLLVQQSVLADFTQRFLALAQTYTCGDPTEEATVVGTVIDEAAAKSLEAAIQDAIT
                     RGAKLLHGGTRQGALLQPTILADVPRDSTLGTSECFGPIAPIFGVRDLNDAIDHANAT
                     PYGLSSGIVTRSLDSAITAIKGIKAGTINVNEIPGYRLELSPFGGVKDSGLGIKEGVV
                     EAIKFMTTVKTYSLPW"
BASE COUNT         1366 a         2720 c         2715 g         1436 t
ORIGIN      
        1 ctgcgcgcgc cgcaggatgg cgtggtgctc acgccggatc tcgaccgcct cgccgccggc
       61 agcctgcgcg ctggcgacac cctctgcacg ctggcggcgg aaggccaggc ggaggtcttt
      121 ttccccctca gccagcgcca ggcgcgccat gtgcgcgccg ggcagaaaat ggagctgcgc
      181 gccgcctcgc gcccgggagt caccttcacc ggcacggtgg ccgaggatgc gaagacgccg
      241 cctgcggaaa gccttccgcc gaacttggcc gccaccctgg gcggcgacat cgccgcgcag
      301 ccggatgaca agggcggact gaagccgctg gaggtgactt acggcgtgct ggtccgcctg
      361 cctgacccgg acggcctcct gcgccccggc atgaccggca cggggcggct gcacggggac
      421 cgggtgacgc tgggcgctgc gctgtggatg cgcctgctgg atttcatcag cctggactac
      481 cgctggtgag cgcggcgcgg cggctcattt gccctcgcgc gcccactgct tcatgaggac
      541 gatgttccgc tccacgtcgg gattgctctt tttcaagtac tcggccatgt cgctgtcata
      601 catggcctcg gattcgggcc ctttgtcccc ggtgcgcgcg atccactcgt caagccgggc
      661 gcggtgggcc tccagctcgg ttttgtagtc gggattggcg gcgaggttgg tgacctgcca
      721 gtggtcgttc acatattcat acagctcctc gatcgcgcgc tccgggctga aaagcagctc
      781 ctcggagacg gagggcagtg tgccggcctc atgcgcggtg cgcagggcgg cgacgatggc
      841 ctttttgtcc ttgtaggcgt tcggctgaag gtgcggacgc cgcggcagga aattgcggat
      901 gtaaagaaag cgctccgtgc gcgcgctgcg gatgcgctcg acggtctcat cgcagcggtc
      961 gcgcgcggca aagacggcct ggcgcggctg gtaatctttc gcgaagatgt cgcgtccctg
     1021 catggcttcc gggatgggaa tgcccgcggc ggccagggac aaggcggcca tgtctatgtg
     1081 ttcgacgaga tccgtgcgca cggcgccttt ggcgatgccg gggccgcgca cgatgaaggg
     1141 gatgtgagct ccctcgtcat agaggaactg ttttccccgc gcgtggctga tgccgtggtc
     1201 ggtcatgaaa atgacgaggg tgttttccag caggccctct ttttccagac gcgcgatgac
     1261 acggccgacg tgcaggtcgg tgaggcggac gctgtcgaga taggcggccc agtcctccag
     1321 cagcacggga tcacgcgggt agtagggcgg cagggtgact ttttgcggat cggtggcgct
     1381 gccaagctcg ctgcgcgcgc gcgcggcgag agctcggttg gcctccagat tgtcggcgcg
     1441 gagtttgccg ccgtggagct gcacctgcat gaagaagggc ttttcaccgc acccggccca
     1501 gtcgtggctg tcatacatgg cggtgtccca ctcgaaattg tagtctgtct tgcccaggcg
     1561 gcctttcatg ggtgcgccgg cggctttttt cttccccgcc ctgccctcgc caaagggcag
     1621 cccgcggaag tcgcgctcct ccaggccgct gccgatgcag gtgaaatagc ccgccttctg
     1681 aaacagctcc ggcacgggcc gcactccctc ggtcaggtgg atcttcaact cgccccgccc
     1741 gctgcggtgg tgatgcgcgc cgatggtcgt ctgatacatg ccggtgatca ttgccgagcg
     1801 gcaggcggag cacacgggcg cggtgacata ggcgcgggtg aagcgcgcgc cctccgccgc
     1861 cagccggtcc accgccggcg tctggatggc cgtctcgccg tagcaggaga agtgcgggga
     1921 catgtcatcc accacgaacc agaggatgtt cggcgcgcgc gcgctggaaa ggagggggag
     1981 aaaggtcagc agggtgagtg gaaggagatg tctcatggca ggcgcgcgcc gtcggaacga
     2041 cagggtgacg aacgggccgg gcggcgattt tttgcacctc atccggcggc agggtgacaa
     2101 ggcgtttgcg cgccccgcgc ccgtgctcca gcatcggcgc atgagcagca tcgccgaacg
     2161 cctcgaaaac atccgcgaac gcatcgccgc agcggcgcgt cacgccgggc gcgcggccgg
     2221 ggaggtggag ctggtggccg tgtcaaagac cttccccgcc gaggccgtgc gcgaggcggc
     2281 ggaggcgggc cagaccctgt tcggagagaa ccgtgtgcag gagctgctgg ccaagcagcc
     2341 ggtgctgccc tcgcgcttgc gctggcatct gatcggcccg ctgcaatcga acaaagtgcg
     2401 caaagtcctg cccgctgcgg acatgcttga ggcggtgcat tccctggaga tcgcccgcga
     2461 catccagcgc gtctccgagg agctgggcct gcggccgcgc gtgctgctgg agatcaatgt
     2521 cgccgccgag tcctcgaagc acggcttcag cccggaccag gcgcgcgcgc ggctggagga
     2581 gctctacggg ctggaccgtc tccagatcga gggactcatg tgcatccccc cctacgattc
     2641 cggcccggag aaaagccgcc gccacttcgc cgccctgcgc gcgctgcgcg gggagctgga
     2701 aaaagccggc ggcgcgccgc tgccgcaact ctccatgggc atgagccacg acttcgaggc
     2761 ggcggtggag gaaggcgcca cgctggtgcg gatcggcagc gccattttcg gctcgcggcc
     2821 gccagcgcgg atgccgcttt gactcgcccg tcatccggcg caatcatccg gcatcgccaa
     2881 accgaagtcc aaacgccgcc gcccgccccc ggggtgcatg agagtcctgc tgctgctgct
     2941 gctggcggtt tttctcgccg catgggtggc cgcgcagtgg gtcttcaaca tccacagcaa
     3001 cctcgtggcc gtctgcgtgc gcgactcgcg cgaaagcctg ctggtgcgcg tgcgcggtga
     3061 cacctggaaa acgcccgcgc agatcactcc cgccgagatg gccgccgcgc tggtggagcg
     3121 ccaccgcgag cccggcctgg tctggagcac ggtgcgcgtg cgccggcccg cgggcctggc
     3181 gggcgcggtg gcgcagaaag tgttcggggc ggaggtgcat gtcgtcacca tctcgcccaa
     3241 ggagttcgag ttcatggcca gctacaaacc cggcttcgaa gtcaccaccg cctccgagcg
     3301 cctcgccacg gagaagctgt ggttcgcggt gaccgccaat ttccgcgatc ccaaggggcg
     3361 cccgctgggc tgggtctggc atgagggccg cgaaaccaac cgcgccttcc ccgaatggag
     3421 cggctgcttt tttgtcaaaa acggcgtgcc gttcttcggc ccgaaatccc tgctcgacga
     3481 ggtgcccggc gccattgagg aaggcgtgca ggtttaccct tcggtgatga agaaccacac
     3541 catcttctcc tatgtgggcc tgaagcccga ccagtacttc gacggcagca agatcagcta
     3601 ccgctccctc ggcggcatga ggcgggacgg cgccatcgtc ttcgtcctct ccggctccgg
     3661 cggcgtcatg aatgtcatgg agaccgcaga gatcgcccgc aagctggaca tccagcacgc
     3721 caccctgctc gatggcggcc gcgccctgca atacagcctg cgcaccgagg acgggccatg
     3781 gcatttcacc tccttcaaca cgcgcgtgaa attcaagccc ggccgcttcg agcagcagct
     3841 ctccccggtc ttcatcggcg tgcgccgcca cgcgcccgag atcatcgccg tgccgccgga
     3901 gtcatgagcc gcatgtgcgg cgcccctcca ggtccgccat cgcgtcatcc acgcgcggaa
     3961 aatcaaaacg gaaacccgcc tccagcgcct tcgcaggcgc ggcccgctgc ccgcccagca
     4021 gcatctcatc cgccatgccg cgcagcagca ggcgcagtcc gaatgccggc gcgtggaaaa
     4081 aagcgggccg cctcaccgcc gcagccagcg cgcgcgtgaa ctgcgcgttc gtcaccgcgc
     4141 ccggcgcgca gacgttcagc gggccgcgcc agctttcatc ctccaccgcc cggaggatca
     4201 tgcgcgccgc gtcccggatg tgaatccacg acatccactg cgcgccgctg ccgagcctgc
     4261 cgcccagccc caggcgaaac acgcgcctca gcagaggata tgccccgccg tccggcccca
     4321 gcaccatgcc cgtccgcagc gtcaccacgc gcatgccggc agcctccgcg ccctgcgccg
     4381 ccgcctccca gcgtccgcag atttccgcca gaaatcccgc gccctgcggc gccgcctcat
     4441 ccaccaccac tccgccactg tccccataaa aacccgcgcc cgaagcgcac accaggactc
     4501 gcggacggtt ttccatcttc catgtcccca gatgcgccac caaggactcg gtgaaatcca
     4561 cgcggcttcg ccagatgcga tcccgctttt cgcgcgtcca caacccggcc agcggctcgc
     4621 ccgccagatg cacgagcgcg tcgagccgcg tctcaggcag cgcgtgggga gcgtctccgg
     4681 gcagtctcaa tgtgcgatcc gcaagctgaa gcgccgcatg gccgcgcgtg taggcgatga
     4741 cttccccgga ctcgcgggca agcgcggtca aatgccgtcc gatgaacccc gtcgctcctg
     4801 tgatgccgat gcgcatgagc ttggatacga gcgtgccggg gaccgtgcgg gaaaatcatt
     4861 cccggcgcca ccagtcggcc tgccgctccc agggggcgcg cgatgcgtcg ggcccggact
     4921 ctccggcacg cagaatctcg tcctcatcgg cctcggccag ttcctggagc agcttttcgt
     4981 ggggttccgg ccgggtcgtg cgccgccaaa tcccccaggc caccgccgcg aagcagccaa
     5041 gcagaaagaa aaagcagcac agggtggcga tcacgaacca gtccagcgcc gccgtgtctg
     5101 ccgtgtgcgc gccgcccgcc ggctggcgca aatgcacgag aaaagcggtc aacggcacgc
     5161 tcatggctca ggaactggca aagaccagcc gcatcgggat catgctcgaa tgcgagaaac
     5221 catgcttggc gaagaacgcc tgcgactcgg cgctgatctt gtcggtaagc agggtgatgc
     5281 ggcggaaatt tttctccctg gcgaattcaa tcgcgtggtc cagaagccgc gccccgtaac
     5341 cctggcggcg gtgctgcgga tgcacgatca catcctccat caggatcacc agcccgccct
     5401 cggccgtgct gatggtgaaa agcaggttca ccatgccgat gacctggtaa tccgtgcgca
     5461 gaacaaaaat ccggccccgg ttcggctgct ccaaaatgag tctcaggcca tgctcctgct
     5521 tggtcctgtc cgggcggaaa tcctcctcct cgctgaacag cgccatcagc aactccacga
     5581 gctggggcat gtcttcaatg gtggcgggtt cgatccgcgg ttcggtgctg atggcgggca
     5641 ttgcagtgat catagccagc cccatgccaa aaggcaatcc cggagaatgc cgctcttccc
     5701 tcactctttt cccaacaccc cctgcggtgt catttacttt tgaggcaaca agataggcgg
     5761 cgggttcagg gcgtcgtgct gacggaatac acgcccttgc gcgcaggcac ttcgagacgc
     5821 ggcaggcggc gtccgccgga ctctccaagc tgaaccggca aggccgcccc cgtttcatcc
     5881 ttcacggcga ttttcgccgc atcccatgcc gccggtggtg tgatctcaat cgtgagcggc
     5941 tggtcgtaga gcgtggcatc cgtggtgcaa tcaagctgga aggcgaggct cttcgcatcg
     6001 gacttcagca gcgtgatctt ggcgctgctg cgctcggttt gatatttgtg gatgtccgcc
     6061 atgccggcga tccagagctg ttcctgatgc gctttggcga tgtcgagagc ggcgcggaag
     6121 ttcgcctcgc tgctgctgag gccctcgccg atgtagtggt aatgcacgcg gcaccacagg
     6181 ccgcgttcaa ggtgggtggc gagcgccttg cggaaggcct cgacacgccc gccatagctg
     6241 tcgtccatgc cgagcgagcc actggaggcg tcgaactgat ggtatttgtc gaggtagtgg
     6301 cgcagcgtgc gcgtggtctg ccactgcgtg ccgccgccaa gattcagcgc caggagcttg
     6361 ctcttgccgg gcgtgagctt ccagatcgcc tccgctgcgc tgccaatttc gcggtccatg
     6421 tcctcgtctc ccttcgcgcc gcggtggtgc gcggaatggt tggccagctc catgtcgccg
     6481 cgttcggcca ccgccgccca ttcggccagc tgggtttcaa aggcatagtt ctgccggctg
     6541 cccgcctcct tgggtcccgg attgaccatg aaggtgccct tgaatccata ctcccccagg
     6601 atggggatgg ccttgctcag atgcgtgggg tgggagtcgt caaatcggat gctcagcgca
     6661 gccgtgcgcc cctgcggcca gttcgcgatg cgcacctctg caccgctgac ctcatcctgg
     6721 atcacccgat ccttctcggc cgtggtctgc ggtgccggaa cggcgggcgg cacggcctgc
     6781 accatgaagg cattcagctt cccggcgacc ggcacgacga tattttccca ctcaatgccg
     6841 gagcgctgca caagcagcgc ctcccctgcc tcatcgcgca cctcgacatc attcgccggc
     6901 cacacctgcc ggcctgattt cggcagctcg acgcgcacgt tcagcggctg cgtctctcca
     6961 tttcgttcca gctgaacgcg cagcttgaac ttgtccaggc tccccacgct gaccgtggcg
     7021 ggtccggaat accgctggag cgaaaccttg acgccgccag cgtcggaggt ctgggcaaga
     7081 gccaaagatg catgaaagct gaacagaagg aaaaaacgaa tccacatgac ggcgagataa
     7141 acaaccatcc cgccaccatt cttccaggtg caggaagcta gctcaccacg gcaacgaata
     7201 cgtcttcacc gtggtcatga acttgatggc ctcaacgacg ccctccttga tgccgaggcc
     7261 gctgtctttg acgccgccga aggggctaag ctcaagacga tagccaggga tttcgttgac
     7321 gttgatggtg ccggctttga tgcctttgat ggcagtgatg gcagagtcca gtgagcgcgt
     7381 gacaatgccg ctgcttaggc cgtaaggcgt cgcattcgca tggtcaatag cgtcattgag
     7441 gtcacgcacg ccaaagatcg gcgcgatggg gccgaagcac tcgctggtgc cgagcgtgga
     7501 gtcgcgcggc acatcggcga gaatcgtggg ctggagcagt gcgccctggc gagtgcctcc
     7561 gtggaggagt ttggcaccgc gtgtaatggc gtcctgaata gcggcttcga gtgacttggc
     7621 ggcggcttca tcaatgacgg tgccaacgac ggttgcctct tcggtgggat caccgcaggt
     7681 gtaggtttgg gcgagcgcga ggaagcgttg ggtgaaatcc gcgagcacgc tttgttgcac
     7741 gaggaggcgg cgcacagcgg tgcagcgctg gccactgttg cggaagcaac cctcgcaggc
     7801 aagcttggcg gcgaggtcga ggtcggcgtc gtcgagcacg atcaagggcg agtgtccgcc
     7861 gagttcgagg caggtttttt tgtaacccgc cgtggcggcg atttctttgc ccactttggc
     7921 gctgccggtg aaggtgacga ggtcaatgcg ctcgtcgcgg atcatcggtc gcgtgacttc
     7981 atcgagattg ccatgaatgc agctcagcaa gtgaccgggc agaccggcct cgtagagcag
     8041 ctcgacgagg cgcacggcgg tgagcggcgt tttttccgag ggcttcaaaa tgactggcgc
     8101 accggcggcg atggcgggcg cgagtttgtg cgcgacttgg ttgagcgggt gattgaaggg
     8161 cgtgatcgcg gcgacgagcg atagcggctc gcgcagggtg aaaatcttgc gcgctttgcc
     8221 ggtgggcgag atgtcgc
//
LOCUS       DAOMYJ010000227         8227 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Prosthecobacter sp. isolate AMR_MDS_5629
            NODE_2904_length_8227_cov_18.902594, whole genome shotgun sequence.
ACCESSION   DAOMYJ010000227 DAOMYJ010000000
VERSION     DAOMYJ010000227.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867850
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prosthecobacter sp. (wastewater metagenome)
  ORGANISM  Prosthecobacter sp.
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Verrucomicrobiaceae; Prosthecobacter.
REFERENCE   1  (bases 1 to 8227)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8227)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.30
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:34:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,742
            CDSs (total)                      :: 4,684
            Genes (coding)                    :: 4,676
            CDSs (with protein)               :: 4,676
            Genes (RNA)                       :: 58
            tRNAs                             :: 55
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8227
                     /organism="Prosthecobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2904_length_8227_cov_18.902594"
                     /isolate="AMR_MDS_5629"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1965333"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..457
                     /locus_tag="PLP58_18255"
     CDS             <1..457
                     /locus_tag="PLP58_18255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ10530.1"
                     /translation="NGGAGFARFEFADDPCLRWFVSRNRFDEIAFKEHLLTSEALRGH
                     LPGLEIPAALEPLEWEWWSSYLLAGDWARSLMQGGAYLKFQEGARKAIETGEKACAEL
                     FGDRHEEVMVFRTGAAWSGWFYNVAWDMTWVGIDKRERKAWLLCLTDTD"
     gene            521..2527
                     /locus_tag="PLP58_18260"
     CDS             521..2527
                     /locus_tag="PLP58_18260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012420018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HRJ10531.1"
                     /translation="MSGFTGTLNPQQRQAATHIHGPLLILAGAGTGKTRVITARIAHM
                     VNEGIPPEHILAVTFTNKAAAEMRERVKDMVRDGLGRKVVLGTFHAFCARVLREFAAQ
                     AGYKSNFVIYSQGEQVSLMKRVLKDLLVKDEKLDAQAALARISKARNHGESLGDPAED
                     LHAAIAERYAAEMRALNVMDFDDLLCLGVRLLEEHADVRALLHQRHRFLMVDEFQDTN
                     SLQMRLLRALAPPPYNVCVVGDDDQSIYGWRGAEITNITEFESFFPNPAVVKLEENYR
                     STTPILHTANSLIVHNAGRRPKKLWSRAHGSDPVRLVAVDDDKEEAEMIAREVERAHF
                     TMREPLESFAVLFRTNDQSRVLEQTFRRHRIAYRVVGARSFYDRREVRDILSYLSALH
                     NPHDDIALLRVLNTPARGIGTVTAELAREWSMERGCSVWVALCDDAFLRQIPEKARLA
                     VRAFTALINKFYQSAQAQGTLLAPMTEMLVAETGYMEHLKKLAKTPEDYQGWENGVGE
                     LLKQITAYDERNRKDGLAGFLDEVSLNDEREDKDDIEKKKGVCLITLHASKGLEFPIV
                     YLPGLEKGILPHKRSYEEGRLDEERRLFYVGITRAQKKLTLSFARTRVKWGKPQTELP
                     SPFLEELDRAHIEELDYSRHMKEHASIEENTSFFAGLKAMLAQD"
     gene            complement(2536..3495)
                     /locus_tag="PLP58_18265"
     CDS             complement(2536..3495)
                     /locus_tag="PLP58_18265"
                     /inference="COORDINATES: protein motif:HMM:NF013408.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I mannose-6-phosphate isomerase"
                     /protein_id="HRJ10532.1"
                     /translation="MTWNRPLLFTPVHQTRVWGGRRLESMFGRALPAAGLPYGESWEL
                     CDRAEAQSSLREPADGCGTLHELWTQKRREVFGADLLGHPAPRYPLLMKILDACEDLS
                     IQVHPPADAAAELGGEPKTEMWFIVHADPGAKLYAGLKAGVDRAAFEKALADGTVADV
                     THVIEPRAGDCVFLPSGRVHAIGAGLVIYEIQQNSDTTYRVFDWNRAGLDGRPRALHI
                     AQSLRSIRFDDFEPAPVTPQPDGTLVGCEHFHVRLRKAAPDAALGAPGEHLVIALVRG
                     ALSVAGVSLKAGDFALIPAAMDAASRKATPGAEDTAWLEVRIP"
     gene            complement(3766..4695)
                     /locus_tag="PLP58_18270"
     CDS             complement(3766..4695)
                     /locus_tag="PLP58_18270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ10533.1"
                     /translation="MSRPPHQENKVEAILDALANAPAHIVPLQLLDEAHDHREALIPP
                     FQQAVAQAHALSAGCRLQAEGDWRLPCFALHFLAAWRVPGTYDLILDSLMLDDRYDSR
                     WLMCDAYNDWPHLLITTFDGDVARLLRILQKEDPPWNSDLRTCLILLLGGVYHHRLGD
                     RAAIEAAFDQMLDTCDSDLIMLTIANCIARAKMKRLLPRLHKMMQDEVVSELDVRGIL
                     DAPWEEGVKNLASCPNPIVNPRQDLADIISMWFYFRPPFVPSPEVKQSKLYQWEIQIN
                     DTTARPGPFILRKRTLPCNCDSGLPYGACCAGK"
     gene            complement(4776..5024)
                     /locus_tag="PLP58_18275"
     CDS             complement(4776..5024)
                     /locus_tag="PLP58_18275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ10534.1"
                     /translation="MRHSRPGNGEEISGQGCRLASVVPASLPEAFREGVRRFCERTPV
                     PIAVHLFVTIDPATGQPDTRQWRLISRLRRARHRRRHR"
     gene            complement(5219..5671)
                     /locus_tag="PLP58_18280"
     CDS             complement(5219..5671)
                     /locus_tag="PLP58_18280"
                     /inference="COORDINATES: protein motif:HMM:NF014173.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HRJ10535.1"
                     /translation="MKLSKKAEYALRALLAMARAPSGTNFSIQDLARRERIPLKFLEQ
                     ILLALRNGGLLRSKRGVGGGYQLQKTAAEISLLEVVRLMDGPFEPVTCSAPAGKRAVK
                     CECGIPGGCGLGNAFATLRAQVDAWLAGTSLETVLAQEKKAETLNFEI"
     gene            complement(5729..7051)
                     /locus_tag="PLP58_18285"
     CDS             complement(5729..7051)
                     /locus_tag="PLP58_18285"
                     /EC_number="1.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012420991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004412 - homoserine dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine dehydrogenase"
                     /protein_id="HRJ10536.1"
                     /translation="MTVPFPQRILHVGLAGLGNVGAGVYKNLEKNRDLILQRTGADIR
                     VRRVVVRDAARARDVAVPAEMLGTDWRALVADPEIQVIVELIGGTGTAYELVCAALRA
                     KKIVVTGNKALLAERGQELFALAEQCGVPIYFEAAVAGGIPIIQVLQEGLVGNRILSI
                     HGIINGTCNYILTRMSQAGLSYADALREAQEKGYAEADPALDVSGWDAAHKAIILASL
                     SYGFWIPQEKVHVQGVEGIDPADFRFAQRLGYTIKLLSVIRADADGQVEVRTQPALVP
                     LSHVLASVSGSFNAVLVNGDIVGETLFYGRGAGQDPTSSSVISDLCEAAAVLLYGARH
                     SGFVPHGLYGKSKPVEDTISHYYLRLTVDDVPGVMAQVAAVLGRRNIGISSMIQPEDL
                     EDTSGSTSIVLMIHDATLGEMKAALEEIGALSCVREKPSWMRVETLQG"
     gene            7246..8001
                     /locus_tag="PLP58_18290"
     CDS             7246..8001
                     /locus_tag="PLP58_18290"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HRJ10537.1"
                     /translation="MIGHGISPALFLGAAFLGAVCARADVVPGILLPLHEVKVGTPVE
                     GIVAEVFVKEGGTVEKGAPLARLVDDLERLDLERAEKVLEKARFDHEAAQRLLKESIG
                     TREEALKKSIEHDLARIQRDAARFRLEQKTLRAPIQGVVVARGRQPGEAALLHETVAH
                     IVHLRTVEAQFFVEPAQAAVLSTGMSMKVRLPAPHAEAEAELVFLDPRVDAESGLCRV
                     KLRLENADLRLTAGMRVEAEFPEKAAGHEQAKP"
     gene            7982..>8227
                     /locus_tag="PLP58_18295"
     CDS             7982..>8227
                     /locus_tag="PLP58_18295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ10538.1"
                     /translation="MSKPSPEPERAPDSLVPPALRRDLETSRQLFEGRAFVIIKDPLS
                     LKYFRLTAEDHALAALFDGRRTLAEIRAAFAAAHPHAA"
BASE COUNT         1402 a         2643 c         2896 g         1286 t
ORIGIN      
        1 gaacggcggg gcgggcttcg cgcgcttcga gttcgcggac gacccgtgcc tgcgctggtt
       61 cgtgagccgg aaccgctttg acgagatcgc cttcaaggag cacctgctga cctcggaggc
      121 gctgcgcggg catctgcccg gcctggagat ccccgccgcg ctggagccgc tggagtggga
      181 gtggtggagc agctacctgc tggcgggcga ctgggcgcgg agcctcatgc aagggggcgc
      241 ctacctgaag ttccaggaag gcgcgcgcaa ggccatcgag accggagaga aggcgtgcgc
      301 ggagctcttc ggcgaccgcc acgaggaggt catggtcttc cgcacgggcg cggcctggag
      361 cggctggttt tacaatgtcg cctgggacat gacctgggtg ggcattgaca agcgggagag
      421 gaaggcctgg ctgctctgcc tgaccgacac ggactgagcg gcgagatttg cccgcttggc
      481 gcttgcgcgg cgcggcagtc cgccgcaaca aggcgcgcgc atgagcggat tcaccggcac
      541 tctcaacccc cagcaacgcc aggcggcgac ccacatccac gggccgctgc tcatcctggc
      601 cggcgcgggc acgggcaaga cgcgcgtgat caccgcgcgc atcgcgcaca tggtcaacga
      661 gggcatcccg ccggagcaca tcctggccgt gaccttcacc aacaaggccg cggcggaaat
      721 gcgcgagcgg gtgaaggaca tggtgcgcga cggcctgggc cgcaaggtgg tgctgggcac
      781 cttccacgcc ttctgcgcgc gcgtgctgcg ggagttcgcc gcgcaggccg gctacaagag
      841 caacttcgtc atctacagcc agggggagca ggtcagcctc atgaagcgcg tgctcaagga
      901 cctgctggtc aaggatgaga agctcgacgc ccaggcggcg ctggcgcgca tcagcaaggc
      961 ccgcaaccac ggcgagtccc tgggcgatcc cgccgaggat ctgcacgccg ccatcgccga
     1021 gcgttacgcc gcggagatgc gcgcgctgaa tgtgatggac ttcgacgacc tgctctgcct
     1081 gggcgtgcgg ctgctggagg agcacgcgga tgtgcgcgcg ctgctgcacc agcgccaccg
     1141 ctttctcatg gtggacgagt tccaggacac gaactccctg caaatgcgcc tgctgcgcgc
     1201 gctggcgccg ccgccataca atgtgtgcgt ggtgggggat gacgaccagt ccatctacgg
     1261 ctggcgcggc gcggagatca cgaacatcac ggagttcgag tccttcttcc ccaaccccgc
     1321 cgtggtgaag ctggaggaga actaccgctc caccacgccc atcctgcaca cggccaacag
     1381 cctcatcgtc cacaacgccg gcaggcgccc gaagaagctg tggagccgcg cgcacgggag
     1441 cgacccggtg cggctggtgg cggtggacga tgacaaggag gaggcggaga tgatcgcgcg
     1501 cgaggtggag cgcgcgcact tcaccatgag ggagccgctg gagtccttcg cggtgctctt
     1561 ccgcaccaac gaccagagcc gcgtgctgga gcagaccttc cgccggcacc gcatcgccta
     1621 ccgcgtggtg ggcgcgcgca gcttctacga ccggcgggag gtgcgggaca ttctcagcta
     1681 cctctccgcg ctgcacaatc cgcatgacga catcgccctg ctgcgcgtgc tgaacacgcc
     1741 ggcgcgcggc atcggcacgg tgaccgcgga gctggcgcgg gagtggtcca tggagcgcgg
     1801 ctgcagcgtc tgggtggcct tgtgcgatga cgccttcctg cgccagatcc ccgagaaggc
     1861 gcgcctggcc gtgcgcgcgt tcaccgcgct catcaacaag ttctaccagt ccgcgcaggc
     1921 gcagggcacg ctgctggcgc cgatgacgga gatgctcgtc gccgagaccg gctacatgga
     1981 gcatctcaaa aagctggcca aaactccgga ggattatcaa ggctgggaga acggcgtggg
     2041 cgagctgctc aagcagatca ccgcctacga cgagcgcaac cgcaaggacg ggctggcggg
     2101 ctttctcgac gaggtctccc tcaacgacga gcgcgaggac aaggacgaca tcgagaagaa
     2161 gaagggcgtg tgcctcatca ctctgcacgc cagcaagggg ctggagttcc ccatcgtcta
     2221 tctgccgggg ctggagaagg gcatcctgcc gcacaagcgc agctatgagg agggccggct
     2281 ggacgaggag cggcggcttt tctatgtggg catcacccgc gcccagaaga agctgaccct
     2341 gtcctttgcg cgcacgcgcg tgaagtgggg caagccgcag accgagctgc ccagcccctt
     2401 cctcgaagag ctggaccgcg cccacatcga ggagctggac tacagccgcc acatgaagga
     2461 gcacgccagc atcgaggaga acacgagctt tttcgccggc ctcaaggcca tgctggcgca
     2521 ggactgagcc gcggctcagg gaatgcgcac ctccagccag gcggtgtcct ccgcgccggg
     2581 ggtggctttg cgcgaggccg cgtccatcgc cgccgggatg agggcgaagt ccccggcctt
     2641 caatgacacg ccggccacgc tgagcgcgcc gcgcacgagg gcgatgacca ggtgctcccc
     2701 cggcgcgccg agagcggcat cgggcgcggc tttgcgcagg cggacatgga agtgctcgca
     2761 gcccaccagc gtgccgtccg gctgcggagt caccggcgcg ggttcgaagt cgtcaaagcg
     2821 gatgctgcgc agggactgcg cgatgtgcaa agcgcgcggc ctgccgtcga gcccggcgcg
     2881 gttccagtca aagacacggt aggtggtgtc gctgttctgc tggatctcgt agatgaccag
     2941 ccccgcgccg atggcatgga cgcggccgct gggcaggaag acgcagtcgc ccgcgcgcgg
     3001 ctcgatgacg tgcgtcacat ccgccaccgt gccatccgcg agggctttct caaaagccgc
     3061 gcggtccact ccggccttca agcctgcgta aagtttcgcg ccaggatcgg catgaacgat
     3121 gaaccacatc tccgtcttcg gctcgccacc cagctcagcg gcggcgtcag cgggcggatg
     3181 cacctggatg gagaggtcct cgcaggcgtc gaggatcttc atcagcagcg ggtagcgcgg
     3241 cgcggggtgg ccgagaaggt ccgcgccgaa aacctcccgg cgcttttgcg tccacaattc
     3301 gtgcagcgtg ccgcagccgt ccgccggctc gcgcagcgag ctctgcgcct ccgcgcggtc
     3361 gcacagctcc caggactcgc catacggcag gcccgccgcg ggcagcgcgc gcccgaacat
     3421 ggactccagg cggcgcccgc cccagacgcg cgtttgatgg acgggagtga aaagcagggg
     3481 acggttccag gtcatgcccc gcagcaatga accgtcccgc cggcaagtca aacccggtgc
     3541 gcccagcctc agaggaactg cggcgcatca aggcatggga cgcggcgcat ccagctccgc
     3601 ctcaaagacg ggggcgggca cctccggctg atcctcgcca cccatccgcc gccccgccac
     3661 aacgaccgcc cgcgtcgcct gacaccgtat ccccggattg agcgaggcag gggggcgaaa
     3721 ccgacacggg ccgggcgcgg cccaagccct tcgctgcatt gtcggtcact tgcccgcaca
     3781 acaggcccca taaggcaggc cgctgtcgca gttgcacggc agagtgcgtt tgcggaggat
     3841 gaaaggaccg ggccgggcgg tggtgtcatt gatctggatt tcccactgat acagcttgct
     3901 ctgcttcacc tcgggtgagg ggacgaaggg cggcctgaaa tagaaccaca tgctgatgat
     3961 gtcggcgaga tcctgccggg gattgacgat gggattgggg caggaggcga gatttttgac
     4021 cccctcctcc cagggggcat cgagtatgcc ccgaacatcc agttcgctga caacctcgtc
     4081 ctgcatcatc ttgtgcagcc tggggagcag acgcttcatc ttggcgcggg cgatgcaatt
     4141 ggcgatggtc aacatgatga ggtcgctatc gcaggtgtcg agcatctgat cgaaggcggc
     4201 ctcgatggca gcgcggtcgc ctaggcggtg gtgatatacc ccgccaagga ggaggatgag
     4261 gcaggttcgc agatcgctgt tccaaggcgg gtcctctttt tgcaggatgc ggagaagccg
     4321 tgccacatcc ccgtcgaagg tggtgatcag gagatgcggc cagtcgttgt aagcgtcgca
     4381 catgagccag cggctgtcgt aacggtcgtc gagcatgagg ctgtccaaga tcagatcata
     4441 ggtcccgggc acacgccagg cggcgagaaa atggagggca aagcagggca gccgccaatc
     4501 accctccgcc tgcaggcggc aaccggctga gagagcatgg gcctgggcga cggcttgctg
     4561 aaaaggcggg atgagggcct cccggtggtc gtgcgcctca tccaagagct gcaggggcac
     4621 gatgtgcgcg ggggcgttgg caagggcatc gaggatggct tcgactttgt tttcttgatg
     4681 gggggggcgt gacatgggaa gggcgtggtg tcgttcaatc ttgaatcacc ggccaaagcg
     4741 gcgtgctttt tcgcaggaaa gcaagcgcgt tttcgtcatc ggtgacgacg ccggtggcga
     4801 gctctgcgca ggcgcgagat gagccgccac tgccgcgtgt cgggctggcc ggtggcggga
     4861 tcaatggtga caaagagatg cacggcgatg ggcacgggcg tgcgctcgca gaagcggcgg
     4921 acgccttcgc gaaaagcctc cgggaggctg gcgggaacga cggaggccag ccggcagccc
     4981 tgcccggaga tctcctcacc gttgccgggg cgcgaatggc gcatgtcccg aatggcagtc
     5041 gccgtgggat gagaattttt cagtttttcc gcagggttct ttggtgaggc gccgggtttg
     5101 cctgaatcat aacgatgcgg caggactctc ctgcgagcgt ctgcgggctc gcaggagcga
     5161 cgaatcttcc gcaccgtcac ggctgagggg cgctcctggc gccctcccct gccccgcgtc
     5221 aaatctcgaa gttcagcgtc tcggcttttt tttcctgggc caggacggtc tccaggctgg
     5281 tgccggcgag ccaggcatcc acttgcgcgc gcagggtggc gaaggcgttg cccaggccgc
     5341 agccgccggg gatgccgcac tcgcatttga cggcgcgctt gccggcgggg gcggagcagg
     5401 tgacgggttc gaaggggccg tccatgagcc gcaccacttc cagcaggctg atctcggcgg
     5461 cggtcttttg cagttggtag ccgccgccga cgccgcgctt gctgcgcagc aggccgccgt
     5521 tgcgcagggc caggaggatc tgctcgagga atttgagcgg gatgcgctcc cgccgcgcaa
     5581 ggtcctggat ggagaagttc gtgccggagg gcgcgcgcgc catggccagc agggcgcgca
     5641 gggcgtattc ggcctttttg gagagcttca tgcgcaggcg ttaggccggg agcggcgcgg
     5701 cttcaaggcg caatagcgcc ctcgcggctc acccttgcag ggtctccacg cgcatccagg
     5761 agggtttttc ccgcacgcag gacagcgcgc cgatctcctc aagcgcggct ttcatctccc
     5821 cgagcgtggc atcgtggatc atgagcacga tggaggtgct gccgctggtg tcctccaggt
     5881 cctcgggctg aatcatcgag gagatgccga tgttccgccg cccaaggacg gcggccacct
     5941 gcgccatgac gcccggcacg tcatccaccg tcaggcgcag gtagtaatgc gagatggtgt
     6001 cctccacggg tttgcttttc ccgtagaggc cgtgcgggac gaagccgctg tggcgcgcgc
     6061 cgtagagaag cacggcggcg gcctcgcaca ggtcgctgat gacggagctg ctcgtcgggt
     6121 cctgcccggc gccgcgcccg taaaagagcg tctcgccgac gatgtccccg ttcaccagca
     6181 cggcgttgaa gctgccgctg acgctggcca gcacatgaga cagcggcacc agcgccggct
     6241 gggtgcgcac ctccacctgg ccgtcggcgt ccgcgcgaat cacggacagc agcttgatgg
     6301 tgtagcccag cctttgcgcg aatctgaagt ccgccgggtc aatgccctcc acgccctgca
     6361 catgcacctt ctcctgcggt atccagaagc cgtagctcaa ggaggcgagg atgatggcct
     6421 tgtgcgccgc gtcccagccg ctgacatcga gcgccgggtc ggcctcggcg tagccttttt
     6481 cctgcgcctc ccgcagcgcg tccgcgtagc tcaggccggc ctggctcatg cgggtgagga
     6541 tgtagttgca ggtgccgttg atgatgccgt ggatggagag gatgcggttt cccaccagcc
     6601 cctcctgcag cacctggatg atggggatgc ccccggccac cgccgcctcg aagtagatcg
     6661 gcacgccgca ctgctccgcc agggcgaaca actcctggcc ccgctcggcc agcagcgcct
     6721 tgttgccggt gaccacgatc tttttcgcgc gcagcgccgc gcacaccagc tcatacgccg
     6781 tgcccgtgcc gccgatcaac tcgacaatga cctgtatctc cgggtcggcc accagcgcgc
     6841 gccagtccgt gccgagcatc tccgcaggca ccgccacatc ccgcgcgcgc gccgcgtccc
     6901 gcaccaccac gcgccgcaca cgaatgtccg cgccggtgcg ctggaggatg aggtcgcggt
     6961 tcttctccag gtttttgtaa actcccgccc ccacgttgcc gagaccggcg aggccgacgt
     7021 ggaggatgcg ctggggaaag gggacggtca tggcgggaaa gaaaaagaaa aatgacttgg
     7081 caacctcaaa gccgagtccc gccgcgaatg caagcgccgc gtgctttccc gcgtgccgcc
     7141 ccggcgcggc ttccggggcg ggtttgatcg aaacgggcaa actggaaggc tgccccactt
     7201 tgcgcggggt tgccgccggg gggaagcgga tgtatctgcg cgggaatgat tggtcacggc
     7261 atttccccgg cacttttcct cggcgcggcc ttcctcggcg cggtctgcgc gcgggcggac
     7321 gtggtgccgg gcatcctgct gccgctgcac gaggtgaagg tgggcacgcc ggtggagggc
     7381 atcgtggcgg aggtttttgt caaagagggc ggcacggtgg aaaagggggc gccgctggcg
     7441 cggctggtgg acgacctgga aaggctggac ctggagcgcg cggagaaggt gctggagaag
     7501 gcgcgcttcg accacgaggc ggcgcagcgg ctgctcaagg agagcattgg cacgcgggag
     7561 gaggcgctga aaaaatccat cgagcacgat ctggcgcgca tccagaggga tgcggcgcgt
     7621 ttccgcctgg agcagaagac actgcgcgcg cccattcaag gggtggtggt ggcgcgcggg
     7681 agacagccgg gggaggcggc gctgctgcat gagacggtgg cgcacatcgt ccacctgcgg
     7741 acggtggagg cgcaattctt cgtggagccg gcgcaggccg ccgtgctgag caccggcatg
     7801 agcatgaaag tgcggttgcc ggccccgcac gcggaggcgg aggctgagct ggtgtttctt
     7861 gacccgcgcg tggatgccga gagcggcctg tgccgggtga agctgcgcct ggaaaacgcg
     7921 gatctgcgcc tgacggcggg catgagggtg gaggcggagt ttccagaaaa agcagccggc
     7981 catgagcaag ccaagccctg agccggagcg cgcgcccgat tccctcgtgc cgcccgctct
     8041 gcggcgggat ctggagacga gccgccaact cttcgagggc cgcgccttcg tcatcatcaa
     8101 ggatccgctg tcattgaaat acttccgcct caccgcggag gaccacgccc tggccgcgct
     8161 cttcgacgga cgccggacgc tggctgagat ccgcgccgcc ttcgccgccg cgcatccgca
     8221 cgcggcc
//
LOCUS       DAOMYJ010000228         8086 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Prosthecobacter sp. isolate AMR_MDS_5629
            NODE_2957_length_8086_cov_16.948574, whole genome shotgun sequence.
ACCESSION   DAOMYJ010000228 DAOMYJ010000000
VERSION     DAOMYJ010000228.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867850
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prosthecobacter sp. (wastewater metagenome)
  ORGANISM  Prosthecobacter sp.
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Verrucomicrobiaceae; Prosthecobacter.
REFERENCE   1  (bases 1 to 8086)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8086)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.30
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:34:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,742
            CDSs (total)                      :: 4,684
            Genes (coding)                    :: 4,676
            CDSs (with protein)               :: 4,676
            Genes (RNA)                       :: 58
            tRNAs                             :: 55
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8086
                     /organism="Prosthecobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2957_length_8086_cov_16.948574"
                     /isolate="AMR_MDS_5629"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1965333"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..273
                     /locus_tag="PLP58_18300"
     CDS             <1..273
                     /locus_tag="PLP58_18300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ10539.1"
                     /translation="PWLEAAAHGGPAAGWRIHFTAWGFPARFEPLSEKPAAEQVTWVR
                     PWTGLHTWRTRQMLTGQGASAQIGPRGEVLLGLVFAADGGTPEPLR"
     gene            complement(571..2547)
                     /locus_tag="PLP58_18305"
     CDS             complement(571..2547)
                     /locus_tag="PLP58_18305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012419511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HRJ10540.1"
                     /translation="MSDPIRHECGIAVVRLKKDLAYYQETKGSALHGFNLLANLMTKQ
                     RNRGQDGIGIGCCKLGMPPGAPYLFRERSDTSVEQMGEVFDGIRKEYKRISRRIDEQR
                     RQERHDTGREQPPFEEDPEAIKREFEMAGEILIGHLRYGTSGSFGKASLHPYLRRATW
                     PTRSLMVMGNFNLTNTAALNHIMMQRGQHPVFGTDTQTVLEEIGFQLDEAHTQLYHEL
                     RDAGMPGAEIPGHISRRLDVPALIEKSAALWDGGYTIVGVIGNGDLFVMRDPNGIRPC
                     FYYENDEVLVFASERVALMSVLEIEAGQVLELPPAHLAVMKADGALTITPFTARRPPR
                     HCSFERIYFSRGNDPDIHHQRKQLGERLVPQLVEAIGDDFEHTVLSFIPNTAETAYYG
                     FLDGMRKTRRNAVKDALLQMIQRGEFDEARLDDLVLRNWPRAEKIAHKDIKMRTFIAQ
                     ETGRDQMVSSVYDITYGVVTPQDNLVVIDDSIVRGTTLRTSLLRILARTHPRRIIVIS
                     TAPQIRYPDCYGIDMSELGKFIAFQAAIELHREHGHRSILRETRAACEAELQKPRDQM
                     GNAVKAIYAPFTDAEISAKIARLIHPQHTPWKGQVQVIFQTIPNLHAALGPGYGDWYF
                     SGDYPTPGGYATVNRAFINFCDKKEGRAYDTTLL"
     gene            complement(2646..4196)
                     /locus_tag="PLP58_18310"
     CDS             complement(2646..4196)
                     /locus_tag="PLP58_18310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ10541.1"
                     /translation="MNEAQSRREFLAGVGKGTLLATIGPALASELGISTAHAVEAPAT
                     LDFGALEPLVRLMQETPPSNLQPALAAKLKEGVPLKKLVAAGVLANARTFGGEDYIGF
                     HTLMALGPCLEMAGAMPEREQALPVFKVLYRNTSRIQENGGRESEVLRPVDAAPGQSA
                     PEDLRASVRSRDTAGAERVFASLMRQDAEAGFNALLECVQDHPEVHRTVLPYRAWDLL
                     DLVGREHATTMLRMSLRYCLEAESRRRPEWDENGKMLARLLDEHKLLGRDPGTRSMED
                     AAVEKLCMDIFKGTPDDAAAAVATALAEGFDPREIGEAISLAANQLVLRDHGRPPEWE
                     SPGKPAGSVHGDSVGVHASDSVHAWRNLSSASRGRNVTACLILGAWQVARDRGGVSAT
                     MLAREPLPSPYHLRRVTRNDQAALLSELDGMIRQNLQSHAAAVVHHWGALGLPERPVL
                     GLLLRYAVSEDGALHAEKYFQTAWDEFRRTRPAFRWRHLTALARVTASEYGRPAPGQA
                     EARALLGV"
     gene            complement(4313..6346)
                     /gene="ligA"
                     /locus_tag="PLP58_18315"
     CDS             complement(4313..6346)
                     /gene="ligA"
                     /locus_tag="PLP58_18315"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009963732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HRJ10542.1"
                     /translation="MTHAEATARAAFLRTDLHRHNRLYYIEAQPEISDQEFDALLREL
                     QDIEATFPDLITPDSPTRRVGGAPIEGFTQVRHTVPMMSLDNTYSEAELASFFQRLQK
                     GLGRESIRCVIEPKVDGVAITVRYENGVLKHAATRGDGQTGDDVSANARTIQSLPLRL
                     PADCPQTLEARGEVFMPKEGFAKLNEERESAGEPRFANPRNATAGTLKQLDPRIVARR
                     PLDIVFYGIVDASADAAAPADDLFSAAPGAPQSQSEVHALLAKAGLRKADLVWKADSA
                     EGLLAAIRELDEKRKTLPYETDGAVIKVDSSADQRELGATSKAPRWAIAYKYQPEQAE
                     TRLLAADIQVGRTGALTPVARLEPVFLSGSTVSNATLHNFEEIERKDIRAGDLVVIEK
                     AGEIIPAVVMVKKESRTGDETAIPRPTHCPVCGTAVHQDEEQVVIRCPNPHCPEVVKR
                     RLEHFCSRGAMDIRGLGESVVAQLVDARLVRDAADLYDLDALKLAGLERQGSKSIENL
                     LKALAESKQQPPWRLVFGLGILHVGVTAARTLIEHFGGVDAIAAASIEELTQCPDIGA
                     VVAPSIHAWFRDPVNAALLDRLRAAGLTFTQRSVEAASDKLKGTTWVLTGALSQPREE
                     IADLIRAHGGKVSGSVSGKTTYLLAGEDAGSKLDKAAKLGVAVVSEEMFRAML"
     gene            6448..7785
                     /locus_tag="PLP58_18320"
     CDS             6448..7785
                     /locus_tag="PLP58_18320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ10543.1"
                     /translation="MNDADRIAHIRRAFPEEGLFRDKEWLLSPEVFALDAATAGIITA
                     LGPALRAFQRACDRLYQEGVENAALAWVTRLLDQGKPPGVIALGRHERWRDELPGVLR
                     PDLVLTEDGVCIAELDSIPGGIGLTGWLAETYSALGFEVAGGADGMVRGFDAAFPRRD
                     ILISREAADYQPEMEWLVSRLGAGGTARGVMNPWNLTPHELAGCEFYRFFELFDLENV
                     EHAEQMLRLAGAGEMSFSPPLKAYLEEKLWLALFHSPQLQDWWEAALTPEHLALLRRC
                     IPRGWVVDPAPLPPHAVLPGLEIHSWAEARTFGGRRRELVLKISGFSELGWGSRGVSV
                     GHDMSLEQWGAALDAALATFPEHPHVLQEFHRGRVVEHPVWDEERQAARLMRARVRLC
                     PYYFVPEGTDETRLGGVLATVCPADKKILHGMRDAAMLPAGFPRKMGHSRDLA"
BASE COUNT         1124 a         2429 c         3058 g         1475 t
ORIGIN      
        1 ccctggctgg aggccgcggc gcatggcggt cccgccgccg gctggcgcat ccacttcacc
       61 gcctggggct tccccgcgcg gttcgagccg ctgtcagaaa agcccgccgc tgaacaggtc
      121 acctgggtgc gcccctggac cggactgcac acctggcgca cccgccagat gctcaccggc
      181 cagggagcgt cggcacagat cggcccgcgc ggggaggtct tgctggggct ggtctttgcc
      241 gcggacggcg gcactccaga gcctctccgg taaacgcgcc aggagccgga gccaggggcg
      301 ctgaccctgg aagacgcgcc caaacgaggt gctgtgaagt gaatgcctga ccttcaatcc
      361 cggccgcttc cgccttctgc catgccgtgc gtcaccatga attgtgattt tccgcagatt
      421 caaggaggcc agcagatttc tctttctcat ccttaatctg tgggcttccc tgaatctgtg
      481 ggagaaaacc caattcacaa aaggtgatga ccttcagtat aaaaacgggc agtccgcttg
      541 agggaacacg cggccgcttt cggggctggg tcagaggagg gtggtgtcgt aggcgcggcc
      601 ttcttttttg tcgcagaagt tgatgaaggc gcggttgacg gtggcgtagc cgccgggggt
      661 ggggtagtcg ccgctgaagt accagtcgcc gtagccgggg ccgagggcgg cgtggaggtt
      721 ggggatggtc tggaagatga cctggacctg gcctttccag ggggtgtgct gggggtggat
      781 gaggcgggcg attttggcgg agatttcggc gtcggtgaag ggggcgtaga tggctttgac
      841 ggcgttgccc atctggtcgc ggggtttttg cagctcggcc tcgcaggcgg cgcgggtctc
      901 gcgcaggatg gagcggtggc cgtgctcgcg gtggagctcg atggcggcct ggaaggcgat
      961 gaatttgccg agttcggaca tgtcgatgcc gtagcagtcg gggtagcgga tctggggggc
     1021 ggtggagatg acgatgatgc ggcggggatg ggtgcgggcg aggatgcgca ggagggaggt
     1081 gcgcagggtg gtgccgcgga cgatggagtc gtctatgacg acgaggttgt cctggggggt
     1141 gacgacgccg taggtgatgt catagacgct ggagaccatc tggtcgcgcc cggtttcctg
     1201 ggcgatgaag gtgcgcattt tgatgtcctt gtgggcgatt ttttcggcgc ggggccagtt
     1261 gcgcaggacg aggtcgtcga ggcgggcctc gtcgaactcg ccgcgctgga tcatctggag
     1321 gagggcgtct ttgacggcgt tgcggcgggt tttgcgcatg ccgtcgagga agccgtagta
     1381 ggcggtctcg gcggtgttgg ggatgaagct gaggacagtg tgctcgaagt cgtcgccgat
     1441 ggcctctacg agctggggca cgaggcgctc gccgagctgc ttgcgctggt ggtggatgtc
     1501 ggggtcgttg ccgcgggaga agtagatgcg ctcgaaggag cagtggcggg gcgggcgccg
     1561 cgctgtgaag ggggtgatgg tgagggcgcc gtcggctttc atgacggcga ggtgggcggg
     1621 gggcagctcg aggacctgcc cggcctcgat ctcgaggacg gacatgaggg cgacgcgctc
     1681 ggaggcgaag acaagcacct cgtcgttctc atagtagaag caggggcgga tgccgttggg
     1741 gtcgcgcatg acgaagaggt cgccgttgcc gatgacgccg acgatggtgt agccgccgtc
     1801 ccagagcgcg gcggattttt cgatgagggc gggcacgtcg agccggcggc tgatgtggcc
     1861 ggggatctcg gcgccgggca tgccggcgtc gcgcagttcg tggtagagct gcgtgtgggc
     1921 ctcgtcgagc tggaagccga tctcctcgag cacggtctgg gtgtcggtgc cgaagacggg
     1981 atgctggccg cgctgcatca tgatgtggtt gagggcggcg gtgttggtga ggttgaagtt
     2041 ccccatgacc atgaggctgc gggtgggcca ggtggcgcgg cgcaggtagg ggtggaggct
     2101 ggctttgcca aagctgccgg aggtgccgta gcggaggtgg ccgatgagga tctcgccggc
     2161 catctcgaac tcgcgcttga tggcctcggg gtcctcctcg aagggcggct gctccctgcc
     2221 ggtgtcgtgg cgctcctggc ggcgctgctc gtcaatgcgg cgggagatgc gcttgtactc
     2281 cttgcggatg ccgtcgaaga cctcgcccat ctgctcgacg gaggtgtcgg agcgctcgcg
     2341 gaagaggtag ggcgcgccgg gcggcatgcc gagcttgcag cagccgatgc cgatgccgtc
     2401 ctggccgcgg ttgcgctgct tggtcatcag gttggccagc aggttgaagc cgtgcagcgc
     2461 gctccccttg gtctcctggt agtaggccag gtcttttttc aaacggacaa cggcaatgcc
     2521 gcattcgtgt cggatggggt cgctcatgca gggtgggagc gcgcaccctt ggcggcgaaa
     2581 ctggcgggcg caacccgtga ggcggttttt tgccgccgct gctgccgcgg gcatggcggg
     2641 tgacgtcaga ctccgagcag cgcgcgcgcc tcggcctggc ctggcgcggg cctgccgtat
     2701 tcgctggcgg tgactctggc cagggcggtg aggtgccgcc agcggaaggc gggccgggtg
     2761 cggcggaact cgtcccaggc ggtctggaaa tatttttccg cgtgcagcgc gccgtcctcg
     2821 ctgacggcgt aacgcagaag caagcccagc acaggccgct ccggcaggcc gagcgcgccc
     2881 cagtgatgca ccacggcggc ggcgtgggac tgcaggttct ggcggatcat gccgtcgagt
     2941 tcggacagca gcgcggcctg gtcattgcgg gtgacgcggc ggaggtggta cggggagggc
     3001 agcggctcgc gcgcgagcat ggttgcgctg actccgcccc ggtcccgcgc cacctgccag
     3061 gcgccgagga tgaggcaggc ggtgacgttc cgcccccggc tggcgctgga gagattgcgc
     3121 caggcgtgga cggagtcgct ggcatgaacg ccgaccgagt cgccatgcac gctgccggcg
     3181 ggcttgccgg gggattccca ctccggcggg cggccgtggt cgcgcaggac gagctggttg
     3241 gcggccaggg agatggcctc gccgatttcc cggggatcaa agccctcggc cagggcggtg
     3301 gcgacggctg cggcggcgtc atcgggcgtg cctttgaaga tgtccatgca cagcttttcc
     3361 accgccgcgt cctccatgct ccgggtgccg gggtcgcgtc cgagcagctt gtgctcgtcg
     3421 agcaggcgtg ccagcatttt gccgttctca tcccactcgg gccgcctgcg gctctcggcc
     3481 tccaggcagt agcgcaggga catgcgcagc atcgtggtgg catgctccct gcccacgagg
     3541 tcgagcaagt cccaggcacg gtagggaagc acggtgcggt ggacttcggg gtggtcctgg
     3601 acgcattcga gcagggcgtt gaacccggcc tccgcgtcct ggcgcatgag cgaggcgaag
     3661 acgcgctcag cacccgcggt gtccctgctg cgcacgcttg cgcgcaagtc ctccggcgcc
     3721 gattgtccgg gcgccgcgtc caccgggcgc aggacttcgg attcgcgccc gccgttttcc
     3781 tggatgcggc tggtgttgcg gtagagcacc ttgaaaacgg gcagcgcctg ctccctctca
     3841 ggcatggcgc ccgccatttc gaggcagggg cccagcgcca tcagcgtgtg aaagccgatg
     3901 tagtcctcgc cgccgaaggt gcgggcgttg gccaggacgc cggccgccac gagttttttc
     3961 aacggcacgc cttccttgag cttcgcggcc agcgccggtt gaagattgga gggcggcgtc
     4021 tcctgcatga ggcgcacgag cggttccagc gcgccaaagt ccagcgtggc tggcgcctcg
     4081 acggcatggg cggtgctgat gcccagctcc gaggccaggg ccggaccgat ggtggcgagc
     4141 agggtgcctt tgccgacacc ggccaggaat tcgcggcggg actgagcttc attcatgatt
     4201 gcctcccttg ttggttgagt ctgacactcc gcccgtccgc cggggattca agggtaattt
     4261 tggttaaact tcggggtccg ccgcctcatc gccgaccatg ccgcgtcatg cctcacagca
     4321 tggcgcggaa catttcctcg ctgacgacgg cgacgccgag cttggcggcc ttatcgagtt
     4381 tgctgccggc gtcctcgccc gcgagcaggt aggtggtctt gccgctgacg ctgccgctga
     4441 ccttgccgcc gtgcgcgcgg atgaggtcgg cgatctcctc gcgcggctgg ctgagcgcgc
     4501 cggtgaggac ccaggtggtg cctttgagct tgtcgctggc ggcttcgacg ctgcgctggg
     4561 tgaaggtgag tccggcggcg cgcaggcggt cgagcagggc ggcgttcacc gggtcgcgga
     4621 accaggcgtg gatgctgggt gcgacgacgg cgccaatgtc ggggcattgc gtcagctcct
     4681 cgatgctggc ggcggcgatg gcgtccacgc cgccgaagtg ctcgatgagc gtgcgcgcgg
     4741 cggtgacgcc cacatgcagg atgccgaggc cgaagacgag ccgccacggg ggctgctgct
     4801 tgctttcggc gagggctttg agcaggtttt caatgctctt gctgccctgc ctttcgaggc
     4861 cggccagctt gagggcatcc aggtcataca aatccgccgc gtcgcgcacg aggcgcgcgt
     4921 ccacgagctg ggcgacgacg gattcaccca ggccgcggat gtccatggcg ccgcgcgagc
     4981 agaagtgctc caggcggcgc ttcaccactt cggggcagtg cgggttcggg cagcggatga
     5041 cgacctgctc ctcatcctga tgcacggctg ttccgcacac cgggcagtgg gtggggcgcg
     5101 ggatggcggt ctcgtctccc gtgcggctct cctttttcac catgaccacg gcggggatga
     5161 tctcgccggc tttttcgatg acgacgaggt cgccggcacg gatgtccttg cgctcgatct
     5221 cctcgaagtt gtgcagggta gcgttgctca cggtggagcc gctgaggaaa accggctcca
     5281 gccgcgccac gggagtcagc gcgccggtgc ggcccacctg gatgtccgcg gcaagcagtc
     5341 gcgtctccgc ctgctccggc tggtatttgt aggcgatggc ccagcgcggg gccttgctgg
     5401 tggcgccgag ctcgcgctgg tcggcggagc tgtccacctt gatcaccgcg ccgtccgtct
     5461 cgtagggcag cgttttgcgt ttctcatcga gctcgcggat cgcggccagc aagccttcgg
     5521 cgctgtcggc tttccagacg aggtcggcct tgcgcaggcc cgccttcgcg agcagggcgt
     5581 ggacttcgct ttgcgactgc ggtgcgccgg gggcggcgga aaaaagatcg tccgcaggcg
     5641 cggcggcatc tgcggacgca tccacgatgc cgtagaagac gatgtcgagc gggcgtctgg
     5701 cgacgatgcg cgggtcgagc tgcttgaggg tgccggcggt ggcgttgcgc gggttggcaa
     5761 agcgcggctc gccggcgctc tcgcgctcct cgttgagctt ggcgaagccc tccttcggca
     5821 tgaagacctc cccccgcgcc tccagcgtct gcgggcagtc cgctggcagg cgcagcggca
     5881 ggctctggat ggtgcgcgca ttcgcggaca cgtcgtcgcc cgtctggccg tcgccgcggg
     5941 tggcggcgtg tttgagcacg ccgttttcat agcgcacggt gatggccacg ccgtccacct
     6001 tcggctcgat gacgcagcgg atgctctcgc ggccgaggcc cttttgcagc ctttgaaaaa
     6061 acgaggccag ctcggcctcg gagtaagtgt tgtccaggct catcatgggc acggtgtgcc
     6121 tgacctgggt gaagccctcg atgggcgcgc cgcccacgcg ccgggtgggc gagtcgggtg
     6181 tgatgaggtc cgggaatgtc gcctcgatgt cctgcagctc gcgcagcagc gcgtcgaact
     6241 cctggtcgct gatctccggc tgcgcttcaa tgtagtaaag gcggttgtgg cggtgcagat
     6301 ccgtcctcag aaatgctgcg cgtgcggtgg cttcggcgtg ggtcatggct gggtgatagc
     6361 agggatgatg aatgatgaaa atatgaaaat gccgggtgga aaatcttgtc gccaaagcgc
     6421 cgggtttcgg caaggagatg gcgactcatg aacgacgccg accgcatcgc gcacatccgc
     6481 cgcgcttttc cggaggaggg gcttttccgt gacaaggaat ggctgctttc tccggaagtc
     6541 ttcgcgctgg acgcggccac ggcggggatc atcacggcgc tggggccggc cttgcgggct
     6601 tttcagcggg cgtgcgacag gctctatcag gaaggtgtgg agaatgccgc gctggcctgg
     6661 gtgacgcggc tgctcgatca aggcaagccg ccgggtgtca tcgcgctcgg ccggcatgag
     6721 cggtggcgtg acgaactgcc cggcgtgctg cgacccgatc tcgtgctcac ggaagacggg
     6781 gtgtgcatcg cggagctgga ctccatcccc ggcggcatcg gcctcactgg ctggctggcg
     6841 gagacttatt cggcgctcgg cttcgaggtg gctggcggcg cggatggcat ggtgcgcggc
     6901 tttgacgcgg cctttccccg gcgggacatc ctcatctcgc gcgaggcggc tgattaccag
     6961 ccggagatgg agtggctggt gtccaggctt ggcgccggcg gcactgcgcg cggggtgatg
     7021 aacccgtgga acctgacacc gcacgagctg gccggatgcg agttttaccg cttcttcgag
     7081 ttgttcgacc tggagaatgt ggagcacgcg gagcagatgc tgcggctggc cggggcgggg
     7141 gagatgagtt tttcgccgcc tttgaaggcg tatctggagg agaagctgtg gctcgcgctt
     7201 tttcactcgc cgcaattgca ggactggtgg gaggccgcgc tgacgccgga gcatctggcg
     7261 ctgctgcggc gctgcatccc gcgcggctgg gtggtggacc ccgcgccgct gcccccgcac
     7321 gcggtgctgc cggggctgga gattcattcg tgggcggagg cgcggacttt cggcgggcgg
     7381 cggcgggaac tggtgctgaa gatcagcggc ttttccgagc tgggctgggg ctctcgcggc
     7441 gtctcggtcg gccacgacat gagcctggag cagtggggcg cggctctcga cgcggcgctg
     7501 gcgactttcc ctgagcatcc gcatgtgctg caggagtttc accgcgggcg ggtggtggag
     7561 catccggtgt gggacgagga gcgccaggcc gcgcggctga tgcgcgcgcg ggtgcggctg
     7621 tgcccgtact atttcgtgcc ggagggcacg gatgagaccc ggcttggcgg cgtgctggcc
     7681 acggtctgcc cggctgacaa aaaaatcctc cacggcatga gggacgcggc gatgctgccc
     7741 gctggtttcc cgcgtaaaat ggggcatagt cgcgacctcg catgattcaa ggcgtcacca
     7801 gcctccgctg tcccgcgtgc gggcagccct ttctttccct gccgcagccg gcgggtgcca
     7861 atgtgacatg cccgcactgc gcccgcagcg cgccggcggg cgcctatgcg cccgcaagcc
     7921 agggcgcgcc tggagtggcg acggcggtgc ccttaagacg gcgggatgcc gcgccgccgc
     7981 agattcagtc ccccttcact tccgcccagc cgccgcgcaa acccgcgctc cctccggcct
     8041 ggcccgccgc gccggagcag gcgccgccat tgataccgcc cgtgct
//
LOCUS       DAOMYJ010000229         7988 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Prosthecobacter sp. isolate AMR_MDS_5629
            NODE_3000_length_7988_cov_22.485945, whole genome shotgun sequence.
ACCESSION   DAOMYJ010000229 DAOMYJ010000000
VERSION     DAOMYJ010000229.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867850
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prosthecobacter sp. (wastewater metagenome)
  ORGANISM  Prosthecobacter sp.
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Verrucomicrobiaceae; Prosthecobacter.
REFERENCE   1  (bases 1 to 7988)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7988)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.30
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:34:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,742
            CDSs (total)                      :: 4,684
            Genes (coding)                    :: 4,676
            CDSs (with protein)               :: 4,676
            Genes (RNA)                       :: 58
            tRNAs                             :: 55
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7988
                     /organism="Prosthecobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3000_length_7988_cov_22.485945"
                     /isolate="AMR_MDS_5629"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1965333"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2569)
                     /locus_tag="PLP58_18325"
     CDS             complement(<1..2569)
                     /locus_tag="PLP58_18325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HRJ10544.1"
                     /translation="MPHCRIFISTVSHEFLTCRERLATDLRFPDVDVQTQEQNVSALA
                     SGQTILIKLDDYIAQCDAVIHLIGRQTSKDGRVAGKEAVDDLLQRHAGLPDVVGLQED
                     ELRTLSYTQWEAWLAYYHRKTKRPELKLIVATPSSAFVPDHPADAATAHAQKLSQAWH
                     EKELKQRGRYSEISFAEAKDLSIAVLRALKAILPAQQPPQRIATTRLISRHTAADFLG
                     RDKELALLDDAWNPPPAQNQEQGTTNLLSIIAWGGVGKTALLAYWVRSRFTEKGWLNA
                     KGQPDPLAYFDWTFYDQGTRGDDATHAGAASLGSFFQKALEHFGDPEPENPEKKAARL
                     ARHLQAQRSLLILDGLEPLQYPPNHPQAGQLTDPDLRELLGLLAQRNPGLCLISSRQP
                     LTDLPSAGTSPTRQHDLEELPLECAVSLLRKMQITGTEEELQQAATDYFCHALSLIVL
                     GRFLFVKGGDIRLRSQLKLERANDNRNQRITRNAWHILEAYEQWLASPQGHPTDVQAL
                     RLTGLFDRPASADCLAALRRAPAIPGLTDALVPLNDDAWNAVLLRLHEAHLIQLRFPP
                     VAEGSFAPRPEPCQVTLDAHPLIREYFAKQLRGVGKKAWWQFWRSKNADAFKMAHSRL
                     FDHLCQSTEHRPDTLEGLQPLYQAVVHGCLAGRQQEAVEKVYDARILRGTGPGGFYSR
                     TKLGAIGADLGAVAAFFDPPWSRLSPNLSEADQAWLLNEAALRLRALGRLTEAREPMR
                     VTLEINAKAKQWENAAAVASNLSELEVTLGLLGEAVATARRAIEFADQSRDAFQRMIN
                     RTTAADALHQSGQRAEARGLFEQAETLQRERQPQFDLLYSLQGFRYSDLILAEAERAA
                     "
     gene            complement(2529..2882)
                     /locus_tag="PLP58_18330"
     CDS             complement(2529..2882)
                     /locus_tag="PLP58_18330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ10545.1"
                     /translation="MPGGIKEPSVAILGCFAIIYPVIAVAALSLAAAFDLEAKKHTSE
                     ERVAFLERQAGLLESAATAREFAKLLIETESRLLGETVNWHARRSFAGVKRAGRNVPS
                     RRHATLPHLHLHSQS"
     gene            3122..4105
                     /locus_tag="PLP58_18335"
     CDS             3122..4105
                     /locus_tag="PLP58_18335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009965127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRJ10546.1"
                     /translation="MPETVKSAFQSRPDGWALVRRNRPAMVSLVFLGFVVLAAFAVPL
                     LLPDGLKGTGEGVFLPPFSPAAGGGVHLCGTDNNGQDLFFRLLTGAQVSLGVGIIGAL
                     ISLCIGTVWGMVSGFCGGRVDALMMRVVDMLYAVPRILFIMIFIAAFDSVCKDWLDAL
                     RLRAQEAGWAGVEGAARQLIPYSKILVMILSLGLVEWLTMARIIRGQVLVLRETAFVT
                     AARALGQSRASILRRHLLPNLSTLILTYLTLTIPAVILDESFLSFLGLGIEDPAASWG
                     SLLKDGAQVINPFESKWWLLAFPAALMSLSLLALNFLGDGLRDAFDPKSGD"
     gene            complement(4119..5240)
                     /locus_tag="PLP58_18340"
     CDS             complement(4119..5240)
                     /locus_tag="PLP58_18340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ10547.1"
                     /translation="MQKPLALARKQTGDIGTALEAGRPALREAARKSAGEPSLLLHIG
                     THKTGSTAIQRALRKNRAVLLKRGIVSQPRCNDALRAMLELGEMDASLCARIAGQLAA
                     FRTAKASSLVVSNEGLCGDPLLGYRNAPDFARRMRSCTAGYKVTVLVYIRRQDEFIES
                     LYTQKIHEGHTLTFEEFLDQISGLVFDWHALLESYAEVFGRENIIVRRYEKRFMPRRE
                     SLLEDFFEITGAGHAGLELRQGDAAPNAGYSRIAAEIARQCNSHLGTEERREMRRVLQ
                     ASIPKQPHESYSHMDGPARASLLARHEASNALVARDYFGDTTGRLFEFLPEEDAGIAS
                     APATGEMLAALVKVLLQTHARRPKSKLGRALVRLGEMMK"
     gene            complement(5336..6817)
                     /gene="glpK"
                     /locus_tag="PLP58_18345"
     CDS             complement(5336..6817)
                     /gene="glpK"
                     /locus_tag="PLP58_18345"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016292161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004370 - glycerol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HRJ10548.1"
                     /translation="MKKYILALDQGTTSSRAIVFDRLGGVVATAQREFTQYYPQPGWV
                     EHDAEEIWSTQLRTMREAVKKAGAKPAEIAAIGITNQRETTVAWDRRSGKPVCRAIVW
                     QDRRTAGFCDQLKARGAEAMIRHKTGLVVDAYFSATKLRWILQNVPGAKELAKAGDLA
                     FGTVDTWLLWNLTGGRVHATDVSNASRTMLYDITRGAWDDELLKLFGVPASVLPQVLP
                     SSGVFGDTGLLGGAIPVTGIAGDQQAALFGQVCVKPGMVKNTYGTGCFMLMNTGAKRI
                     ASGNNLLTTVAWQLGGSAPVFAVEGSVFIAGAVVQWLRDGLGIIKKSSEVEALAASVP
                     DTGGVHLVPAFAGLGAPHWDPHARGILCGMTRGTTRAHIARAALEGIAFQVSDILQAM
                     QADAGVRLRELRVDGGASNNNLLMEFQAGLLGVPVVRPKVTETTALGAAYLAGLAAGF
                     WKSQAEIATQWQADRRFEPGIKAAERRALTAGWSRALERCKGA"
     gene            7179..>7988
                     /locus_tag="PLP58_18350"
     CDS             7179..>7988
                     /locus_tag="PLP58_18350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ10549.1"
                     /translation="MTDVSAKGDEFSLHDGDACVSVIVGEGAAAPEFGMEVEIEGVVV
                     AEIFMDRKRVRIKAAKVSVLGAGTLPEPETVRVEDVAKFKHLDQWVSVEGMVLQVRSS
                     MSLFTIQMVDEAASCNVLVRDWPRDAIPQDWIGGRVRVAGVNRAHLPGSGFLSLTASS
                     PDQVTVLKTGVVDPLDAPSATLEALRGGEHGGQERVKITGTLLGATMGNVFYARGAGG
                     GGFSFYMLHPIDEDKSGRFSTPIIMPKVQPGDELEVTGVPGRGAPGVHLNFG"
BASE COUNT         1253 a         2451 c         2810 g         1474 t
ORIGIN      
        1 acgccgcgcg ctcggcctcg gccaggatca gatcgctgta gcggaagcct tgcagggagt
       61 agagcaggtc aaactggggc tggcgctcgc gctggagcgt ctccgcctgc tcgaagaggc
      121 cgcgcgcctc cgcccgctgc ccgctctggt gcagggcatc ggcggcggtg gtgcggttga
      181 tcatgcgctg aaacgcatcc ctgctctggt cggcgaactc gatggcgcgc cgggccgtgg
      241 ccaccgcctc ccccagcagg cccagcgtca cttccagctc gctgaggttg ctggccactg
      301 cggcagcgtt ttcccactgt ttagctttcg cgttgatttc caatgtcacc cgcatcggct
      361 cccgcgcttc ggtgaggcgg ccgagggcgc ggaggcggag cgcggcttca ttgagcagcc
      421 aggcctgatc ggcttcgctg agattcgggg agaggcggct ccagggcggg tcaaagaagg
      481 ccgccaccgc ccccaggtcc gcgccgatgg ctccgagttt cgtcctgctg tagaagccgc
      541 cggggccagt gccgcggagg atgcgggcgt cataaacctt ttccaccgcc tcctgctgcc
      601 gcccggccag gcagccgtgg accacggcct ggtagagcgg ctggaggccc tccagggtgt
      661 cgggccggtg ctcggtgctc tggcacaggt ggtcaaagag ccgggagtgc gccattttga
      721 acgcgtcggc attctttgac ctccagaact gccaccacgc cttctttcca accccgcgca
      781 gttgcttggc gaagtattcg cggatgagcg ggtgggcgtc gagggtcacc tggcatggct
      841 cggggcgcgg ggcgaaactg ccctccgcca ccggcgggaa gcggagctgg atgaggtggg
      901 cctcgtgcag gcgcagcagc acggcattcc aggcgtcgtc attcagcggc acgagggcgt
      961 cggtgaggcc ggggatggcg ggggcgcggc gcagggcggc cagacagtcg gcgctggccg
     1021 ggcggtcgaa caggccggtg aggcgcaggg cctgcacatc ggtggggtgg ccctgggggg
     1081 aggccagcca ttgctcatag gcctccagga tgtgccaggc attgcgggtg atgcgctggt
     1141 tgcggttgtc attggcgcgc tcgagcttga gctggctgcg caggcggatg tcgccgcctt
     1201 tgacgaagag gaagcggccg agcacgatga ggctgagggc gtggcagaag tagtcggtgg
     1261 cggcctgctg gagttcttcc tccgtgccgg tgatctgcat tttccgcagg agggagacgg
     1321 cgcactccag cggcagctcc tccagatcat gctggcgggt ggggctggtg ccggcgctgg
     1381 ggaggtcggt gaggggctgg cggctggaga tgaggcagag gccggggttg cgctgggcca
     1441 gcaggccgag cagctcgcgc aggtcgggat cggtgagctg gccggcctgg gggtggttgg
     1501 gcgggtattg caggggctcc aggccatcga ggatgagcag gctgcgctgc gcctggaggt
     1561 gccgggcgag gcgggcggcc tttttctccg ggttctccgg ctcgggatcg ccgaagtgct
     1621 ccagggcttt ctggaagaag ctgccgaggc tggcggcccc ggcgtgggtg gcgtcgtcac
     1681 cacgggtgcc ctggtcgtag aaggtccagt cgaaataggc gagcgggtcg ggctggcctt
     1741 tggcattgag ccagcctttc tccgtgaagc ggctgcgcac ccaatacgcc agcagggcgg
     1801 tcttccccac gccgccccag gcgatgatgg agaggaggtt ggtggttcct tgttcctggt
     1861 tctgggctgg gggcgggttc caggcgtcgt cgagcagggc gagttccttg tcgcgcccga
     1921 ggaagtcggc ggcggtgtgg cggctgatga ggcgggtggt ggcgatgcgc tgcgggggct
     1981 gctgggcggg gaggatggcc ttcagcgcgc gcagcacggc gatggagagg tctttggcct
     2041 cggcgaagga gatttccgag tagcggcctc gttgtttcaa ctccttctca tgccaggcct
     2101 gggagagctt ttgcgcatga gcggtggcgg catcggcggg gtggtcggga acaaacgcag
     2161 aagagggcgt cgcgacgatg agctttagtt cggggcgctt tgtcttgcgg tggtagtagg
     2221 ccagccaggc ttcccattgg gtgtaggaga gggtgcggag ctcatcctcc tgcaaaccga
     2281 cgacgtcggg caagccggcg tgacgttgca ggaggtcatc caccgcttct ttgccggcca
     2341 ccctgccgtc cttgctggtc tgccggccga tgagatgaat caccgcatca cactgggcga
     2401 tgtagtcgtc cagcttgatg aggatggtct ggcctgaggc gagagcggag acgttttgct
     2461 cctgcgtctg cacatccaca tcgggaaagc ggagatcggt ggcgagccgc tcacggcagg
     2521 tgaggaactc atgactgact gtggagatga agatgcggca gtgtggcatg gcggcgggag
     2581 ggaacgttgc gaccggcacg cttcacgccg gcgaagctgc ggcgggcgtg ccagttgacg
     2641 gtctcgccca ggaggcggct ttcggtctcg atgaggagtt tggcgaactc gcgcgcggtg
     2701 gcggcgcttt ccagcaggcc ggcctggcgc tccaggaagg cgaccctttc ctcggaggtg
     2761 tgctttttgg cctccaggtc aaaggcggcg gccagggaca gggcggcgac ggcgatcacg
     2821 ggatagatga tggcgaagca gccgaggatg gcgacagagg gctctttgat gccgccgggc
     2881 acaggcaggc agccgcactg ggtcagcagc ttcgccagcg tgccagggac tgcgagtggt
     2941 gggggcggac acgcccaccc tgctgcgcgt gcgcgaccat gtgccccagg cgccaggcgt
     3001 gcgccgcttc atgcgcctgc gcgtgcagcg ttgaaagcag cgggcatttt ctccagcctt
     3061 cacacggctt ccggcaaatc cgccctggca aaaacgccgc cgtccgcttc aatggctcat
     3121 catgcccgag actgtgaaaa gcgcgttcca aagccggccc gacggctggg cgctcgtgcg
     3181 gcggaaccgg cccgcgatgg tctcgctcgt ttttctcggc ttcgtggtgc tggccgcctt
     3241 tgctgttccg ctgctgctgc cagacggact caaaggcacc ggcgagggcg tctttctgcc
     3301 tcctttctca cccgccgctg gcggcggcgt gcatttgtgc ggcaccgaca acaacggcca
     3361 ggaccttttt ttccggctgc tcaccggcgc gcaggtctcc ctcggtgtcg gcatcatcgg
     3421 cgcgctcatc tccctgtgca tcggcacggt gtggggcatg gtcagcggtt tctgcggcgg
     3481 cagggtggac gccttgatga tgcgcgtggt ggacatgctg tatgccgtgc cccgaatcct
     3541 cttcatcatg attttcatcg ccgcctttga cagcgtgtgc aaggactggc tggacgcgct
     3601 gcggctgcgc gcgcaggaag cgggctgggc cggtgtcgag ggagccgcgc ggcagttgat
     3661 cccgtattcg aaaattctcg tcatgatcct ctccctgggc ctcgtcgagt ggctgacgat
     3721 ggcgcgcatc atccgcggcc aggtgctggt gctcagggag acggcgttcg tgaccgccgc
     3781 gcgcgctttg gggcagagcc gcgcctccat tcttcggagg cacctgctgc ccaatctgag
     3841 caccctcatt ctcacctacc tcaccctgac catcccggcg gtgatcctgg acgagtcctt
     3901 cctcagtttt ctcggcctcg gcatcgagga cccggcggca agctggggtt ccctgctcaa
     3961 ggatggcgcg caggtcatca accccttcga gagcaaatgg tggctgctgg ccttccccgc
     4021 cgcgctcatg tccctgagct tgctggcact caattttctc ggcgacggac tgcgagacgc
     4081 cttcgatccg aagtccgggg actgagaggg cgccaagatt atttcatcat ctcaccgagg
     4141 cgcaccaaag cacggcccag ttttgacttc ggacggcgcg catgagtctg aagcagcact
     4201 ttgacaagtg cggccagcat ttccccagtc gccggcgcgg acgcgatccc ggcgtcctct
     4261 tcgggcaaga attcaaaaag ccgcccggta gtgtcgccaa aatagtcgcg cgccaccagc
     4321 gcgttggacg cctcatgccg cgccagaagg gaggcgcgcg ccggtccgtc catgtgcgag
     4381 tagctttcat gcggctgctt ggggatggag gcctgcagga cgcgccgcat ctcccgcctt
     4441 tcctcggtgc cgaggtgcga gttgcactgc cgggcgatct ccgccgcgat ccggctgtag
     4501 ccggcgttgg gggcggcatc accctgcctc aattccagcc ccgcatgacc ggcgccggtg
     4561 atctcgaaga agtcctccag caggctctca cgccttggca tgaagcgctt ttcgtagcgc
     4621 cggacgatga tgttttcacg tccgaaaacc tccgcgtagc tctccagcag cgcgtgccag
     4681 tcgaagacga gacctgaaat ctggtcgagg aactcctcaa aggtcaaagt gtgcccctca
     4741 tgaatcttct gggtgtagag agactcgatg aactcgtcct ggcgcctgat gtaaacaaga
     4801 accgtgacct tgtaaccggc ggtgcagctc ctcattctgc gcgcgaagtc gggcgcgttc
     4861 cgatagccca gaagagggtc gccgcacaga ccctcgttgc tcaccacaag cgaggacgct
     4921 tttgccgtgc ggaaggcggc aagctggccg gcgatccgcg cgcagaggga ggcgtccatc
     4981 tccccgagtt cgagcatggc gcgcagggcg tcgttgcacc tcggctggga gacgatgccg
     5041 cgcttgagga gaaccgcccg gttcttgcgc aaagccctct ggatggccgt gctgcctgtc
     5101 ttgtgagtgc cgatgtgcag cagcagggaa ggttccccgg cactcttgcg cgccgcttcg
     5161 cgaagcgccg gacgccccgc ttcgagagct gttccgatgt ctcccgtctg ctttctggcc
     5221 agggccaaag gtttttgcat ggcggtgagg taggcgacgg atttggagag tcaactggaa
     5281 gcgcggggtg cgaatcaaag gcggccagga ctttatgacg gcatgaagcc gcgctttacg
     5341 cgcctttgca gcgttccagc gcgcggctcc agccggcggt gagagcgcgg cgttctgcgg
     5401 ctttgatgcc gggttcgaag cgcctgtcgg cctgccactg ggtggcgatc tcggcctggc
     5461 ttttccaaaa acccgcggcg agacccgcca ggtaggccgc gccgagcgcg gtggtctccg
     5521 tgaccttggg gcgcaccacc ggcacgccga gcagaccagc ctggaactcc atgagcaggt
     5581 tgttgttgct ggcgcctccg tccacgcgca gttcgcgcag tctgactccc gcgtcagcct
     5641 gcatggcctg gaggatgtcc gacacctgga aagcgatgcc ctccagcgcg gcgcgcgcga
     5701 tgtgggcgcg cgtggtaccg cgcgtcatgc cgcagaggat gccccgcgcg tgcgggtccc
     5761 aatgcggcgc gcccagcccg gcgaaggcgg gcaccagatg cacgccgccc gtgtccggca
     5821 cgctggcggc cagcgcctcc acctcggagg attttttgat gatgccaaga ccgtcccgca
     5881 gccactggac gaccgcgccg gcaatgaaga cactgccctc cacggcgaac accggcgcgg
     5941 agccgccgag ctgccaggcc acggtggtga gcagattgtt gccggaggcg atgcgcttcg
     6001 cgcccgtgtt catgagcatg aagcagccgg tgccataggt gttcttcacc atgcccggct
     6061 tcacgcaaac ctggccaaag agcgccgcct gctggtcgcc cgcgatgccg gtgacgggga
     6121 tggcgccgcc aagcaggccg gtatcgccaa agacgccgct ggagggcagc acctgcggca
     6181 gcacggaggc gggcacgcca aagagcttga gcaactcatc atcccacgct ccacgcgtga
     6241 tgtcatagag catggtgcgt gaggcgttgc tgacatccgt ggcatggacg cggcctccgg
     6301 tcagattcca caacagccag gtgtccactg tgccaaaggc cagatcgcct gccttcgcca
     6361 gttccttggc gccaggcaca ttctgcaaaa tccagcgcag ctttgtggcg gaaaaatagg
     6421 cgtccaccac cagcccggtc ttgtggcgga tcatcgcctc ggctccgcgc gccttgagct
     6481 ggtcgcaaaa acccgccgtg cgccggtcct gccagacgat ggcgcggcag accggctttc
     6541 cgctgcggcg gtcccaggcc accgtggttt cgcgctggtt ggtgatgccg attgcggcga
     6601 tctccgcagg tttcgcaccc gcttttttca cggcctccct catggtgcgc agttgcgtgg
     6661 accagatctc ctcagcatca tgctccaccc agccgggctg cggataatac tgggtgaact
     6721 cgcgctgcgc ggtggcaacg actccgccaa ggcggtcaaa aacgatggca cggctgctgg
     6781 tggtgccctg gtcgagggcg aggatgtatt ttttcatcgc gcggattctg atgaatccgg
     6841 cgttgcttgg cgagcacatt tccgcgcatt gcgctccttc cgccccccgg gggaacaggc
     6901 cgccttatga atcgtccttg gcaaaagaag cggcgggcgg ctaccatgcg caaatattga
     6961 attcgcgttt cccaaatcat gccggccgcc aggcaagtcc ggacttcccg ggctttcgga
     7021 gctttacccc cgcgcttttt ctcctgcttg cggcctggtc cgcgcatcct ctttggggac
     7081 aaaccgaccg cgcggaactg acccgggtga gggaggcgct gacgccggca accggtgagg
     7141 cggcagcggg aacaacgcgg gcaaggctgc gcggtgtcgt gacggatgtc tccgccaaag
     7201 gggacgagtt ttcactccat gacggtgacg cgtgcgtttc tgtgatcgtg ggagaaggcg
     7261 cggcggcgcc tgaattcggc atggaggtcg agatcgaggg cgtcgtggtg gcggaaatct
     7321 tcatggatag aaagcgcgtg cgaatcaagg cggcaaaggt cagcgtactg ggagccggca
     7381 cgctcccgga gcctgagacg gtgagagtcg aggatgtcgc caaattcaaa cacctggacc
     7441 agtgggtgag cgtggagggc atggtgctcc aggtgcgctc ctccatgtct cttttcacca
     7501 tccagatggt ggacgaggcc gcctcttgca atgtgctcgt gcgcgactgg ccgcgtgacg
     7561 cgatcccgca ggactggatc ggcggcaggg tgcgcgtggc aggggtgaac cgcgcgcatc
     7621 taccgggcag cggttttctc tcgctcacgg catcgtctcc ggatcaggtg acggttttga
     7681 aaacgggcgt cgttgacccg ctggatgctc cctcagccac gctggaggct ctgcgcggcg
     7741 gcgagcatgg agggcaggag cgtgtgaaga tcaccggcac cctgctgggg gcgacgatgg
     7801 gaaatgtctt ctatgcgcgc ggcgcgggcg gcggcggttt ctccttctac atgctgcatc
     7861 cgattgatga ggacaaaagc gggcgttttt ccacgccgat catcatgccc aaggtccagc
     7921 cgggtgacga gttggaagtg accggcgtgc caggcagggg cgcgcccggc gtgcatctga
     7981 actttggc
//
LOCUS       DAOMYJ010000230         7977 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Prosthecobacter sp. isolate AMR_MDS_5629
            NODE_3004_length_7977_cov_19.646301, whole genome shotgun sequence.
ACCESSION   DAOMYJ010000230 DAOMYJ010000000
VERSION     DAOMYJ010000230.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867850
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prosthecobacter sp. (wastewater metagenome)
  ORGANISM  Prosthecobacter sp.
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Verrucomicrobiaceae; Prosthecobacter.
REFERENCE   1  (bases 1 to 7977)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7977)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.30
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:34:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,742
            CDSs (total)                      :: 4,684
            Genes (coding)                    :: 4,676
            CDSs (with protein)               :: 4,676
            Genes (RNA)                       :: 58
            tRNAs                             :: 55
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7977
                     /organism="Prosthecobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3004_length_7977_cov_19.646301"
                     /isolate="AMR_MDS_5629"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1965333"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..212
                     /locus_tag="PLP58_18355"
     CDS             <1..212
                     /locus_tag="PLP58_18355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ10550.1"
                     /translation="VTHVPGQSVTHLPGPNRPGCKHASRPNPPAKLGQRSFPQVAKGA
                     VRDAYKEFTPLRGKPALPSEIVTVA"
     gene            complement(442..789)
                     /locus_tag="PLP58_18360"
     CDS             complement(442..789)
                     /locus_tag="PLP58_18360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ10551.1"
                     /translation="MKLSSIAERLVFLDSDTTSDTVADVLSGLLAKAEGWANGEPRYF
                     TTEVLSIVYSFNELARQFSFEPAKNPGLAQRYTELRAVVMDWLRGKEIQTSEERWKQL
                     LLLAQDILVWTEV"
     gene            complement(1288..4833)
                     /locus_tag="PLP58_18365"
     CDS             complement(1288..4833)
                     /locus_tag="PLP58_18365"
                     /inference="COORDINATES: protein motif:HMM:NF019262.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ10552.1"
                     /translation="MNLRPALLTLAALATASAQESEKITYEDHIKPLLENKCFSCHNP
                     DKKRGDLDLTSFGAVMTGGGGGAVVDAGNAEGSRLWTTCAKKEEPFMPPEGTPLNEAE
                     LKILAAWVSGGLLETKSSIAKKSTKPKVDMAVAVTSGRPEGPIARPEHVLLEPVLVAP
                     RTTAVTAMAASPWTSLVAIAGVKQILLYDTDTRQLAGIFPYTEGYARSLRFSRNGSLL
                     VMGGGRGGKMGHAVVWDVKTGKRVIEAGAEFDQVMSADISPDHKKVVIGSPSKKVKVY
                     DTATGEELYVINKHTEWVLGTAFSPDGVLLGTCDRNGNIMVWEASNGGEFYILGQHKA
                     ACNDLAWRADSNVLASCSADGVIMVWEMNEGKKLKEWAAHGGGTQSVAFTPDGNILST
                     GNDGLTRLWDINGNKKGEAPSQGDIVTKVVAHHDSRLAITGNWRGEIKLWTIGEKMTE
                     AGQLSSNPPLIAQRIIESEKLAAALTGQLPEKDAAVAKAVEGVKAAEARLADAKKKAA
                     DTQARVTLLETELKDLPGKITAADKTAADAQAKHKAHAEVIKKHEAQKAARAVKQKEL
                     DGILARIKDAQSTPGDPKMSELSQSLETKKAEVAALDKDLAAAPAPLADLEKAGKTAQ
                     DQAAALKNSKPAKEKELADLKKALEAAPKSIADTEAQVAAAKAALAAAQTALEDHKKQ
                     IAVHQQLPTILKAAQFNVGVLAEKEALAKLEGEINDHTAAQKENEEAKAASITRLESS
                     RKALAEAGNLLPQHEATLNRLKAELDGIVKATEPTRAAEAAARAKVDEQKKTLAAREA
                     ELAALAKARDEGIAAAKKSAEDIGKTIAGLKTKLTEVAGKLSAAEAPVKAAKEKLAQA
                     EAALAAAKKTESDLTAALPAREKAVKAAETALPGFVSELQNAEKARTDLGSAIAENGR
                     AIQSARTGVATLEKTLAEARAKNQEVVGQEKALADARAQLAKLEAQAAESAMKMAEAE
                     GNIGSKRDVRAKAEKTLAEARAALEKSRKEHEAAKAAITAADKALAPAKAAVAAADKT
                     AAPLRGQHESISKEITAQESARAAKLAQVKTREDDFAAKAKPLNEAIAQIKTALQPLE
                     AAHAEAKAKLDADTQIVEAKKAETAKAQADLDSTRKKKTDAETAIAAAEKDIPARDQT
                     LAEINAGLARLQPALDPLRAKVRQLEEQYLTMLPK"
     gene            complement(4985..7798)
                     /locus_tag="PLP58_18370"
     CDS             complement(4985..7798)
                     /locus_tag="PLP58_18370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009961569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1553 domain-containing protein"
                     /protein_id="HRJ10553.1"
                     /translation="MTRAHPLKLLGAAALFAPVIAAANPADFAKVQAIFESKCLECHN
                     PGKVKGDLLMHTAEALLKGGDTGPAIVSGKPDESELVKRMSLPGDHDDIMPPKGGPLP
                     AADIELVKAWIAQGAQWPQGIVMRHKSAEELKAGADLNARLASIKKLEIFPEKFALET
                     RRDHHQVVVMATFADATTRDVTRFSNLSVSDPKLVSLDGDVIKPLADTGAAELTASVG
                     GLTAKAPVEIKGGQQDRTVSFHLDVMPVFLRGGCNQGGCHGAARGKDGFRLSLFGMDP
                     AGDYTRLTREMVGRRINLAIPEESTLVEKAVGSVPHSGNQCYTPESQYNQTILEWISN
                     GAPQDPKEIAKVTGIEVYPKAIVLEGTGNTQQMTVRATYSDGTDRDVTQLALFMSNND
                     PVASITKDGLITSGERGAAFMLARFDVYSVTAQVLVIPAKLEYERPKVAETNYIDTLV
                     NENLHKLRILPSGICTDEEFVRRAHIDVVGLYPKPDDVRAFLADANPKKREALIDSLL
                     QRKEFTELWVMKWAELLQIRSNIPGNNNRPPFYKNALLYHNWLSDRIGKNVPLDEIVI
                     ELLSATGGTVSNPPVNFYQTELDQLKLTENVAQVFMGMRIQCAQCHNHPFDRWTMDDY
                     YGFKAFFSQIGRKNTDDPQEVIIFNSNSGESRHFLTQAVMKPKFLGGDTPEIKPGEDR
                     RRVLAEWLASPKNPYFARNIANITWAHFFGLGIVDPVDDVRVSNPPSNPELLAALAEN
                     LVTSKYDMRKLVKDICTSMTYQRSTKVNETNASDSKNFSHAQVRRVRAEVLLDAISQI
                     TDTPNKFQGLPLGARAVQIADGAVSNYFLTTFGRAKRESVCSCEVKMEPTLSQALHLM
                     NGDAVNDRIRQGRVVAKLIEAKKTDREIIEDLYLRVFGRLPLDKEWQPIQQTLAAAPD
                     QRQQALEDVFWALMNSKEFYFNH"
BASE COUNT         1185 a         2192 c         2813 g         1787 t
ORIGIN      
        1 gtgtaaccca tgtgcccggt caatctgtta cccatctgcc cggtccgaac cgacctggct
       61 gcaagcacgc cagccggccc aacccgccag caaagctggg tcagcgctcc tttccacaag
      121 tcgccaaagg cgctgtgcgt gacgcttaca aggagttcac accgctgcgc ggaaaaccgg
      181 cgctgcccag cgagatcgtg accgtcgcct gaagcagcag caccgtcgtg aaaacccggc
      241 gcagcaaacc cagccgcctc gtttcgaaac gacccagctc aatcgcagca caccccaact
      301 tcacacccgc gatcatccgc agtcccaatc ttcctgcaac ttttcacttc agcaagaccc
      361 acggcaaagc catccacatc cacttgtcaa gaatcgacgc ctgacccttt cttggaagtc
      421 gggctaacgc ggaacgaaag gtcacacttc agtccaaaca aggatatctt gagccaaaag
      481 cagcaactgc ttccatcgct cctcgcttgt ttggatttcc tttcccctca accaatccat
      541 tacgaccgct cgcagttcgg tatatctttg ggcaagaccg ggatttttcg ctggttcaaa
      601 ggaaaactgg cgagccagtt cattgaagga atacacaatc gagagaactt ccgtggtgaa
      661 ataccgaggt tcaccattcg cccaaccctc agctttagcc aacaatccac tcagcacgtc
      721 cgccacagtg tcggatgttg tgtcactatc tagaaaaact aatcgctctg caatagaact
      781 cagcttcatg gctttaaaag gttgggactc cgaacacatt ccgccaaaat ttccattcgc
      841 cgacatgatc gccgctgcct ctcattcgct taacaggctc aggattcagc atagtgtcgt
      901 aaaacaaatg cgcgcccttc ccgtacatca caacaccttc gccagctaat tgggcttcgc
      961 caactcccgg caagaaccct gcaccctcgt aaactgccga cccgtattct ccagcctgga
     1021 ttttcgaact aactgaggaa acggggaaac ggggtcagtg tgcaactgct tgacaaattt
     1081 aatgatcttc attcggctgt ggcgtcggtt taatggtggc cagcttctca tcactttccc
     1141 gtctgtccag ctcgcgcagt tccgccctcg cgcaccaact gcctgccaat tgacgcgttg
     1201 gcgtcttcaa tcaggaaagc cggtggccgc gcatgccgga tcgggcagcg gctccttgca
     1261 aggcccttgc aggggctttg caggaggtca cttcggcagc atggtgaggt actgctcctc
     1321 aagctgcctg accttggcgc gcaggggatc aagggcgggc tggagcctgg cgagtccggc
     1381 gttgatttcg gcgagggttt ggtcgcgcgc ggggatgtct ttttcagcgg cggcgatggc
     1441 agtctcggcg tcggttttct ttttcctggt ggaatcgagg tcggcctggg ctttggcggt
     1501 ctcggctttt ttggcttcga cgatttgcgt gtcggcgtcg agcttggcct tggcttcggc
     1561 atgggcggct tcaagcggct gcaaggcggt tttgatctgg gcgatggcct cgttcagggg
     1621 cttggctttg gcggcgaagt cgtcctcgcg ggttttgacc tgggcgagct tggcggcgcg
     1681 ggcggactcc tgggcggtga tttccttgga gatgctctca tgctggccgc gcagtggggc
     1741 ggcggttttg tcggctgcgg ccacggcggc tttggcgggc gcgagggctt tgtcggcggc
     1801 ggtgatggcg gccttggcgg cttcgtgctc tttgcggctt ttttcgaggg cggcgcgggc
     1861 ttcggcgagg gtcttctcgg ccttggcgcg cacgtcgcgt ttggagccga tgtttccctc
     1921 ggcctcggcc attttcatgg cggactcggc ggcctgggct tcgagcttgg caagctgggc
     1981 gcgggcgtcg gcgagggctt tttcctggcc gaccacctcc tggttcttgg cgcgggcttc
     2041 ggcgagggtc ttttcgaggg tggcgacacc ggtgcgggcg gactgtatgg cgcggccgtt
     2101 ttcagcgatg gcgctgccga ggtcggtgcg ggctttttcg gcgttttgca gttcgctgac
     2161 aaagccgggc agtgcggtct cggcggcttt gacggccttc tcacgcgcgg gcagggcggc
     2221 ggtgaggtcg gattcggtct ttttggcggc ggcgagggcg gcctcggcct gggcgagttt
     2281 ttctttggcg gctttgacgg gggcctcggc ggcgctgagc ttgccggcga cttcggtgag
     2341 cttggttttc agtcctgcga tggtcttgcc gatgtcttcg gcggattttt tggcggcggc
     2401 gatgccttca tcgcgggctt tggcgagggc ggcgagctcg gcctcgcgtg cggcgagggt
     2461 ctttttctgc tcgtccactt tggcgcgggc ggcggcttcg gcggcgcggg tgggttcggt
     2521 ggctttgacg atgccatcga gctcggcttt gaggcggttc aaggtggcct cgtgctgggg
     2581 cagcagattg ccggcctcgg ccagggcttt gcgggagctt tccaggcggg tgatgctggc
     2641 ggctttggct tcctcgttct ctttttgggc ggcggtgtgg tcgttgatct cgccttcgag
     2701 tttggccagg gcctcttttt cggcgagcac gccgacgttg aactgcgcgg ccttcaaaat
     2761 cgtggggagc tgctggtgga cggcgatctg ctttttgtgg tcctcaagcg cggtctgggc
     2821 ggcggcgagg gcggccttgg cggcagccac ctgcgcctcg gtgtcggcga tggatttggg
     2881 cgcggcttcg agggccttct tcaaatcggc gagttctttc tccttggcgg gcttggagtt
     2941 cttgagggcg gcggcctggt cctgggcggt tttgccggct ttttcaaggt cggccagcgg
     3001 cgcgggtgcg gcggcgaggt ctttgtcgag ggcggcaact tcggcttttt tggtctcgag
     3061 cgactggctc aactcgctca ttttgggatc accaggcgtg ctttgggcgt ccttgatgcg
     3121 ggcgaggatg ccatcgagtt ccttttgttt cacggcgcgc gcggcttttt gggcctcatg
     3181 ctttttgatg acttccgcgt gggctttgtg cttcgcctgg gcgtcggcgg cggttttgtc
     3241 ggcggcggtg attttgccgg gcaggtcttt caactcagtt tcgagcaggg tgacgcgcgc
     3301 ctgggtgtcg gcggctttct ttttggcgtc ggccagcctg gcttcggcgg ctttgacgcc
     3361 ctcgacggct ttggcgacgg cggcgtcttt ttcggggagc tggccggtga gggcggcggc
     3421 gagtttttcg gactcgatga tgcgctgggc gatgaggggc gggttgctgg agagctggcc
     3481 ggcctcggtc attttttcgc cgatggtcca gagcttgatc tcgccgcgcc agttgccggt
     3541 gatggcgagc ctggagtcat gatgggcgac gactttggtg acgatgtcgc cctggcttgg
     3601 cgcctcgcct tttttgttgc cgttgatgtc ccagaggcgg gtgaggccgt cattgccagt
     3661 tgaaagaatg tttccgtcag gtgtgaaggc gacggactgg gtgccgccgc cgtgggcggc
     3721 ccattccttg agctttttgc cctcgttcat ctcccagacc atgatgacgc cgtctgcgga
     3781 gcaggaggcg agcacgttgg agtcggcgcg ccaggcgagg tcgttgcagg cggccttgtg
     3841 ctggccgagg atgtagaatt cgccgccgtt ggaggcctcc cagaccatga tgttgccgtt
     3901 gcggtcgcag gtgccgagca aaacgccatc cgggctgaag gcggtgccga gcacccattc
     3961 ggtgtgcttg ttgatgacgt aaagctcctc gccagtggcg gtgtcataca ccttcacttt
     4021 cttcgacggg ctgccgatga cgactttctt gtggtcgggg ctgatgtcgg cgctcatgac
     4081 ctggtcgaac tcggcgccgg cttcgatgac gcgtttcccc gttttgacat cccagacaac
     4141 ggcgtggccc attttccccc cgcgccctcc gcccatgaca agcagggagc cgttccggct
     4201 gaaacgcagg ctgcgcgcgt agccctcggt gtaggggaag atgccggcga gctggcgcgt
     4261 gtcggtgtca taaagcagga tctgcttcac tccggcgatg gcgaccaggg aggtccaggg
     4321 gctggcggcc atggcggtga cggcggtggt gcgcggcgcg acgaggacgg gctccagcag
     4381 cacatgctcg ggcctggcga tcggtccctc ggggcggccg ctggtgacgg cgacggccat
     4441 gtccaccttc ggtttggtgg attttttcgc gatgctggac ttggtctcca acaagccgcc
     4501 gctgacccag gcggcgagga ttttcaattc ggcctcgttg agcggcgtgc cctcgggcgg
     4561 catgaacggc tcctcttttt tggcgcaggt ggtccaaagc cggctgccct ccgcgttgcc
     4621 cgcgtccacg acggccccgc cgcctccgcc ggtcatgacc gcgccgaaac tggtgaggtc
     4681 gagatcgccg cgctttttgt ccgggttgtg gcaggagaag catttgttct ccagcagcgg
     4741 cttgatgtga tcctcatagg tgatcttttc cgactcctgc gcggaggcgg tggcgagtgc
     4801 ggccagggtg aggagggcgg ggcggaggtt catggggatg aattttaaat tcgaaattcg
     4861 aagctcgaaa ttcgatggct ctgggtttct tggagggcgg agggcgggag gacctgaaga
     4921 agggcgggca aggttcgatc ttcgaagttc aagtttcgga cttcgttttc cgccgcaggc
     4981 ggcgtcagtg attgaaataa aactccttgg agttcatgag cgcccagaaa acgtcctcaa
     5041 gggcctgctg acgctggtcg ggcgcggcgg cgagggtctg ctggatgggc tgccattcct
     5101 tgtcgagggg caggcggccg aagacgcgga ggtagaggtc ctcaatgatc tcgcggtcgg
     5161 tctttttggc ttcgatcagc ttggcgacga ccctgccctg acggatgcgg tcgttgacgg
     5221 cgtcgccatt catgaggtgg agcgcctggg aaagggtggg ttccattttg acctcgcaag
     5281 agcatacgga ctcgcgcttg gcgcggccga aggtggtgag gaagtagttg ctcacggcgc
     5341 cgtcggcaat ctgcacggcg cgcgcgccga ggggcaggcc ctggaatttg ttcggcgtgt
     5401 cggtgatctg ggagatggcg tcgagcagga cctcggcgcg gacgcggcgg acctgggcgt
     5461 gggagaagtt tttgctgtcg gaggcgttgg tctcgttgac cttggtgctg cgctggtagg
     5521 tcatggaggt gcagatgtcc ttcaccagct tgcgcatgtc gtatttggag gtgacgagat
     5581 tttccgcgag ggcggcgagc agctcgggat tggacggcgg attggaaaca cgcacgtcat
     5641 ccacggggtc cacgatgccg aggccgaaaa agtgcgccca ggtgatgttg gcgatgttgc
     5701 gcgcgaagta ggggttcttc ggcgaggcga gccattcggc gagcacgcgg cggcggtcct
     5761 cgcccggctt gatctcgggg gtgtcgccgc cgaggaactt gggcttcatc accgcctggg
     5821 tgaggaagtg gcgggactcg ccggagttgc tgttgaagat aatgacctcc tgcggatcgt
     5881 cggtgttttt gcggccgatc tggctgaaga aagccttgaa tccgtagtag tcatccatgg
     5941 tccagcggtc gaagggatgg ttgtggcact gggcgcactg gatgcgcatg cccatgaaga
     6001 cctgggcgac gttttcggtg agcttgagct ggtcgagctc ggtctggtag aagttcacag
     6061 gcgggttcga gacggtgcct cccgtggcgg agagcagctc gatgacgatc tcgtcaaggg
     6121 gcacgttttt gccgatgcgg tcagacagcc agttgtggta aagcagcgcg ttcttgtaga
     6181 agggcgggcg gttgttgttg ccggggatgt tcgagcggat ttgcagcagc tcggcccact
     6241 tcatgaccca gagttcggtg aattccttgc gctggagcag gctgtcaatg agggcctccc
     6301 gcttcttcgg attggcgtcg gcgaggaagg cgcgcacatc gtccggcttt ggataaaggc
     6361 cgacgacgtc aatgtgggcg cggcggacga actcctcgtc ggtgcagatg ccggagggca
     6421 ggatgcgcag cttgtggagg ttctcattga ccagggtgtc aatgtagttg gtctcggcga
     6481 ctttcgggcg ctcgtattcg agcttggcgg ggatgaccag cacctgggcg gtgacgctgt
     6541 aaacgtcgaa gcgcgcgagc atgaaagccg cgccgcgctc gccggaggtg atgagaccgt
     6601 ccttggtgat gctggcgacg gggtcgttat tggacatgaa aagcgcgagc tgggtcacat
     6661 cgcggtccgt gccgtcggag taggtggcgc ggacggtcat ctgctgggtg ttgcccgtgc
     6721 cttcgaggac gatggctttc gggtaaacct caatgccggt gactttggcg atctccttgg
     6781 gatcctgggg cgcgccgttg ctgatccatt ctaggatggt ctggttgtac tgggactccg
     6841 gggtatagca ctggttgccc gagtgcggca cggaaccgac ggctttctcc accagggtgg
     6901 actcctcggg gatggcgagg ttgatgcggc ggccgaccat ttcacgcgtg aggcgggtgt
     6961 agtcgccggc ggggtccatg ccgaacaggc tgaggcggaa gccgtccttg ccgcgggcgg
     7021 cgccatggca gccgccctgg ttgcagccgc cgcgcaggaa gacgggcatg acgtccaggt
     7081 ggaaggagac ggtgcggtcc tgttgtccgc ccttgatctc gacgggcgct ttggcggtca
     7141 ggccgcctac gctggcggtg agttcggcgg cgccggtgtc ggcgagcggt ttgatcacat
     7201 cgccatccag ggagacgagc ttgggatcgg aaacgctcaa gttcgaaaag cgggtgacat
     7261 cgcgcgtggt ggcgtcggcg aaggtggcca tgacgacgac ctgatgatgg tcgcgcctgg
     7321 tttcgagggc gaatttttcg ggaaaaatct ccagcttctt gatggaggcc agacgggcgt
     7381 tgagatcggc cccggctttg agttcctcgg cggatttatg ccgcatgacg atgccctggg
     7441 gccactgggc tccctgggcg atccaggctt tgaccagctc aatgtccgcc gcggggaggg
     7501 ggccgccctt gggcggcatg atgtcgtcat gatcgccagg gaggctcatg cgcttgacca
     7561 gctcgctctc atccggcttg ccggacacga tggccgggcc ggtgtcgccg cctttgagca
     7621 gggcctcggc ggtgtgcatg aggaggtcgc ccttcacctt gccggggttg tggcactcga
     7681 ggcatttgct ttcgaaaatg gcctgcactt tggcaaagtc cgccggattg gccgcggcga
     7741 tgacgggcgc gaacagcgcg gcggctccga gcagcttgag agggtgggca cgagtcatag
     7801 tggggggctt tgtgggaagg tacctacgtt ggcggcgggg aattttcaca atcggatcat
     7861 ttgccggcgg cttcctggcg gagctgctca aggcgggaaa ggggcttggg cgctggaggg
     7921 ggggcagggg cggcctcctt gggcttggcc tcggcgacga cgggctgggc gggcggc
//
LOCUS       DAOMYJ010000231         7961 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Prosthecobacter sp. isolate AMR_MDS_5629
            NODE_3011_length_7961_cov_22.420061, whole genome shotgun sequence.
ACCESSION   DAOMYJ010000231 DAOMYJ010000000
VERSION     DAOMYJ010000231.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867850
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prosthecobacter sp. (wastewater metagenome)
  ORGANISM  Prosthecobacter sp.
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Verrucomicrobiaceae; Prosthecobacter.
REFERENCE   1  (bases 1 to 7961)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7961)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.30
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:34:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,742
            CDSs (total)                      :: 4,684
            Genes (coding)                    :: 4,676
            CDSs (with protein)               :: 4,676
            Genes (RNA)                       :: 58
            tRNAs                             :: 55
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7961
                     /organism="Prosthecobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3011_length_7961_cov_22.420061"
                     /isolate="AMR_MDS_5629"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1965333"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..582
                     /locus_tag="PLP58_18375"
     CDS             <1..582
                     /locus_tag="PLP58_18375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ10554.1"
                     /translation="PAAAPAPAAAPVPRKLELLVLLPEPRVLGTEYSVNPEGARHTVF
                     SPAREVAEVPGIQTYSKEEFQKLGISPETFTERAKATADRLLADIQPDLVKDAEGRVL
                     YAVYRSERPLMASLVTAPSLAATFEKLFGLEIWVALPDRHSLFVFPADPDALEEYTMD
                     LNERWRADTHSASPEIFTLKKGEAPKVVGSFGS"
     gene            complement(577..2064)
                     /locus_tag="PLP58_18380"
     CDS             complement(577..2064)
                     /locus_tag="PLP58_18380"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HRJ10555.1"
                     /translation="MRLFPRSIRWRLHLWHGLLLAVVLCGFGWTVHRLEWTNELRRVD
                     AELQQRMNTLLGLLGRPPGPQGRPPGPPRAETLEDFFPLPEDARPPPPRQDRNFDALS
                     RWVKAGRQQRRWFIAWSREGGILVAEGVDKNPRSKPDTTRMEPLTPYGFTVRAAGEAP
                     VRELMALTPPGDVVLVGRCLLTEAAASRRSALRFAGLGGLVWLLGVAGGGWLTTRALR
                     PIQTVTDTAAAISAGHLDSRINVEETDTELGRMAGVLNDTFSRLRQAFDQQARFTADA
                     AHELRTPVTVMLSQIQLALNRERSPQEYRGTLDTCQRAAERMSCLIESLLELAQMDAG
                     ADPLEVTESDLATVAADCAAMMESLAAERGLVLEKQLSCAPCRVDEKRAAQVITNFLS
                     NALRHSPSGGVVTVRAGVTDGRAWVSVADQGPGIQMEHRARLFDRFYRVENSRNRGSG
                     GAGLGLAICKAIADAHGAVISIEDADGGGSVFTFTMPTDCASAAS"
     gene            complement(2061..2714)
                     /locus_tag="PLP58_18385"
     CDS             complement(2061..2714)
                     /locus_tag="PLP58_18385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009960898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HRJ10556.1"
                     /translation="MRILIVEDEPDLLRTLAQALREEGHAVDEAEDGEEGLFKAVETD
                     YDAVVLDLMLPVMDGFEVLRRLREKRATPVLVLTARGRVSDRVRGLDLGADDYLPKPF
                     DLRELCARLRALCRRSAGETRSEIVLGQVRLDMRSRTLTRDGEPVTLTAREYALVEHL
                     GLHHGKVVTRTELYEHLFDENESSLSNLLDVHVSNIRKKLGHDFITTRRGHGYCIEA"
     gene            complement(2815..3519)
                     /locus_tag="PLP58_18390"
     CDS             complement(2815..3519)
                     /locus_tag="PLP58_18390"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HRJ10557.1"
                     /translation="MTSSFHDTRWTLVSRSRGSDGEARAALSELCEAYYAPVVAFLKR
                     DGREEDVARELAHDFFAKLLGGGAIEGADPVRGRFRSYLLSALKRFAADQRDKTNAAK
                     RGGGQIHADVAEGAEIADLSAEAPDTAFDRQWALTVLARALALLEAELRESGKGHHFE
                     TLKPWLTADADATPQSEAATKLGLSEDAVKVAIHRLRKRFRDAVKAEIAQTVSDLSNV
                     REELDALMAALRGQPC"
     gene            complement(3516..5813)
                     /locus_tag="PLP58_18395"
     CDS             complement(3516..5813)
                     /locus_tag="PLP58_18395"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HRJ10558.1"
                     /translation="MAAAAMPTDGGADTIPLQRADLPSLEEIAVAFPDLEILGLIGHG
                     GMSAVFKARQPKLDRVVALKVLPKSLAATPGFAERFNREGRVLARLNHPSIVAVHDFG
                     ESGGFAYLIMEFVDGVNLRQAMRAGRFTPEQALRIIPAICDALQFAHTQGVLHRDIKP
                     ENILLDSKGRVKIADFGIAKILDEKGGDAMLLTQSGAKLGTAPYMAPEQIEQPSSVDH
                     RADIYSLGVVFYEMLTGELPLGRFAAPSEIAAVGGNIDEIVFRALEKERTRRQQSVSE
                     FKTQVEGAHAAPGRAAVRGNRWDAFEYKSRRTLWGLPLLHVVHGRDPATGKRRQAHGF
                     FAFGDLAKGVFAFGGIARGFFACGGLAVGVVSFGGLSTGLISIGGLAVALLLAVGGVA
                     VGALAMGGVAMGWMACGGVAVGVYAHGGVAAGNHGMGGVVGAGTVVKSISELPPALQW
                     MIRSSPYLTALNLLWLPLGLLMTLVPWWARRQLQVECGGTTREQAGSSSRIFWTIPAT
                     TLFCGMLFWLVGKWSTAVPGGMANIIIPTVICGMGLLFFVLALPLWLRLVPINPVYGV
                     RLPSTMASETRWFAVNAVFGKHLFWWSLLVMGAGVAGFYQLPRHLDAYAWAAITLTLV
                     ALAAAVVSTVVWMRRHPMGATPRKTHRLLRNLELVLPVVVLMFFVRSFVGRAYVVPNG
                     AEPGVTKNSRWLASHLNTGFAAGDLIAYEHESGQTWLARVVTVELKGLRLKRGGNPDE
                     FFMPWDKVIGKMLFSYLSPSAIPKP"
     gene            complement(5931..7211)
                     /locus_tag="PLP58_18400"
     CDS             complement(5931..7211)
                     /locus_tag="PLP58_18400"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009960581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004151 - dihydroorotase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HRJ10559.1"
                     /translation="MKRLYRQAQIANEDSAQLQTADVLIEGERILRVAHGITGADDAE
                     VIDCAGRVLMPAMFDIHVHAREPGQEDKENIATCAEAAINGGVTGFIMMPNTSPAIDN
                     AGVVRSVLESARRTRIGPGIFTSGAITKGRKGEELAGIAGMKAAGAVLLTDDGFPVDN
                     PQVLRRAMEYAREFDLPLASHCEVKELSGKGCMHEGKISYALGLPGIPSISEEICISR
                     DIRLAEFTGVHLNIQHVTTAEGMRTIKRAKDRGIRVTCEIAPHHLMFNHEDIGDYDTH
                     YKMNPPLRTPEDNAALLQGLKDGWFDVIATDHAPHTPFEKNQDFASAPFGITGLETAL
                     VSLYDRFISKGILDWRLIVKRYSAEPRRLFKLPPVPIQEGGIAEFIIFNPARTTTFTR
                     EFMKSKSVNTPFLDQTLQGMVERVIYRGEELLTR"
     gene            7436..>7961
                     /locus_tag="PLP58_18405"
     CDS             7436..>7961
                     /locus_tag="PLP58_18405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018968890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HRJ10560.1"
                     /translation="MKLTLRKSNERGHADHGWLDSYHTFSFADYYDPAHMGFRSLRVI
                     NQDIIAPGAGFPTHPHADMEIFSYILWGALAHKDSMGNGRTLLPGQIQLMSAGRGVTH
                     SEFNPSKTEGASLLQIWIRPHQRGLTPSYTEWHPDSTKENEPKVLVISPDGREGSATI
                     HQDADIYRVRLPKGQ"
BASE COUNT         1483 a         2634 c         2486 g         1358 t
ORIGIN      
        1 cctgcggccg cgccggcgcc ggccgctgct cccgtgccgc gcaagctgga gctcctggtc
       61 ctgctgccgg agccgcgagt gctgggcacc gagtactccg tcaatcccga gggcgcgcgc
      121 cacaccgtct tctctcccgc tcgtgaagtg gccgaggtgc ctggcattca gacctattcc
      181 aaggaagaat tccaaaagct gggcatcagc ccggagactt tcaccgagcg cgccaaagcc
      241 accgcggacc ggctgctggc agacatccag cccgacttgg tgaaggacgc cgagggcagg
      301 gtgttgtacg ccgtttaccg cagcgagcgg ccgctcatgg catcgcttgt gacagccccc
      361 tctctggcgg cgaccttcga aaaactcttc ggcctggaaa tctgggtggc gctgcctgac
      421 aggcactcgc tctttgtatt tccggcggat ccggacgcgc tggaggaata cacgatggac
      481 ctcaacgaac gctggagggc cgacactcat tcggccagcc cggagatctt cactttgaaa
      541 aaaggcgagg ctcccaaagt ggtgggcagc ttcggctcat gaggcggcgc tcgcacagtc
      601 ggtcggcatc gtgaatgtga acacgctccc gccgccatcg gcgtcttcga tggaaatgac
      661 cgcgccgtgc gcgtccgcga tggctttgca gatcgccagg ccaagccccg ctccgccgct
      721 gccccggttg cgcgagttct caacccggta aaagcggtcg aaaagccgcg cgcggtgctc
      781 catctggatg cctggcccct ggtccgccac gctcacccag gcgcgcccgt cagtgacgcc
      841 ggcgcggact gtgacgacgc ccccggacgg cgaatgccgg agcgcgttgg aaaggaaatt
      901 ggtgatgacc tgggcggcgc gcttttcatc cacacggcag ggtgcgcagg agagctgctt
      961 ttccaggacc agtccgcgct ccgctgccag ggactccatc atggcggcgc aatccgcagc
     1021 caccgtggca aggtcggact cggtcacttc aaggggatcg gcacctgcgt ccatctgcgc
     1081 gagttcgagc agggattcaa tgaggcagct catgcgttcg gcggcgcgct ggcaggtgtc
     1141 cagagtgccg cggtactcct gcgggctgcg ctcacggtta agggccagtt ggatttgtga
     1201 gagcatcact gtcaccggcg tgcgcagctc atgcgcggcg tcggcggtga agcgcgcctg
     1261 ctggtcgaaa gcctggcgca gacgggagaa ggtgtcattg agcacaccgg ccatccggcc
     1321 aagctcggta tctgtctcct ccacgttgat ccggctgtcg agatgccccg ccgagatggc
     1381 cgccgccgtg tccgtgactg tctggatggg gcgcaatgcg cgggtggtga gccagccgcc
     1441 gcccgccact ccaagcagcc agaccagccc gcccagtccc gcaaagcgca aggcggagcg
     1501 cctgctcgcc gccgcctccg tcagcaggca gcggcccacc agcaccacat cgcccggtgg
     1561 ggtgagggcc atcagctcgc gcacaggagc ctcgccagca gctcgcaccg tgaagccgta
     1621 gggggtgagg ggctccatgc gggtcgtgtc cggtttggac ctcgggttct tatccacacc
     1681 ctcggcgacc aggatgcctc cctcgcgcga ccatgcgatg aaccaccgcc gctgctgcct
     1741 gcccgccttc acccagcggc tcaatgcatc gaagttcctg tcctggcgcg ggggcggcgg
     1801 acgggcatcc tccggcaggg gaaaaaaatc ctcaagtgtc tccgcgcgcg gcggacccgg
     1861 cggcctgccc tgggggccgg gcgggcgtcc gagcaggccg agcagcgtgt tcattctctg
     1921 ctgcagttcg gcgtccaccc gccgcagttc gttggtccat tccaggcgat ggaccgtcca
     1981 gccgaagccg cagagcacga cggcgagcag cagcccgtgc cagagatgca ggcgccagcg
     2041 gatggagcgc gggaaaagtc tcatgcctca atgcagtagc cgtggccgcg gcgggtggtg
     2101 atgaagtcat ggccgagctt tttgcggata ttggagacat gaacatcgag caggttgctg
     2161 aggctgctct cgttttcatc gaagagatgc tcatacagct cggtgcgggt gacgaccttg
     2221 ccgtgatgca ggccaagatg ctcgaccagg gcgtactcgc gcgcggtgag ggtcacaggc
     2281 tcgccatcgc gtgtgagagt gcgcgagcgc atgtccagac gcacctgccc gagcacgatt
     2341 tcgctgcgcg tctcgccggc ggagcgccgg cacagcgcgc gcagcctggc gcagagctcg
     2401 cgcaggtcga agggcttcgg cagataatca tccgcgccca ggtccagccc gcgcacgcgg
     2461 tcgctcactc tgccgcgcgc ggtgagcacg agcacagggg tggcgcgctt ttcacgcagc
     2521 cgccgcagca cctcgaagcc gtccatcacc ggcagcatga ggtcgagcac caccgcgtcg
     2581 tagtccgtct ccaccgcctt gaacaaaccc tcctcgccat cctccgcctc gtccacggca
     2641 tggccttcct cccgcagcgc ctgggccagc gtgcgcagca gatccggctc atcctcgaca
     2701 atcagaatcc tcatgacttg atgagaatac cccgccccgc cttaagcaag catgaagaac
     2761 aggctggcgg caaaggccat gccgggtttc ctggcatcga aaccggggac atcttcaaca
     2821 gggctgccct ctcagcgccg ccatcagcgc gtccagttct tcgcgaacat tgctcaaatc
     2881 actcaccgtt tgcgcgatct cagccttcac cgcgtcgcga aaacgctttc gcagccggtg
     2941 aatggccact ttgacggcgt cttcactgag gccgagcttc gtcgctgcct cgctttgagg
     3001 ggttgcatcc gcatcggcgg tcagccaggg cttcagcgtc tcaaaatgat gtcctttgcc
     3061 actctcgcgc aattcagcct ccagcaaggc cagagcacga gccagcaccg ttagcgccca
     3121 ttggcggtca aaagccgtgt caggcgcttc agcgctcaaa tcggcgattt cggctccttc
     3181 ggcaacatcc gcgtggattt ggccgcctcc gcgcttcgcg gcgtttgtct tgtcgcgctg
     3241 gtccgcggcg aaacgcttca gcgcggaaag caaatagctg cggaatctcc cgcgcaccgg
     3301 gtccgcgccc tcaatggccc cgccgccgag aagcttcgca aaaaaatcat gcgccagctc
     3361 gcgcgccaca tcctcctcgc ggccgtcccg tttcaaaaac gccaccaccg gcgcatagta
     3421 ggcctcgcac agctcgctga gcgccgcccg cgcctcacca tcgcttccgc gtgagcgaga
     3481 gacgagcgtc catcgggtgt catggaagga ggaagtcatg gtttgggaat cgccgatgga
     3541 gacagatacg aaaacagcat cttcccaatc actttgtccc acggcatgaa aaactcatcc
     3601 ggattaccgc cgcgcttcaa acgcaggcct ttgagctcaa ccgtcaccac gcgagccagc
     3661 cacgtctggc cgctctcgtg ctcgtaggca atgaggtcgc ctgcggcgaa tcctgtgttg
     3721 agatgtgacg cgagccagcg gctgtttttg gtgacgccag gttcggcgcc gttcggcacg
     3781 acgtaggccc ggcccacgaa cgagcgcacg aagaacataa gcaccacgac cggcaggacg
     3841 agttcgagat tgcgtaggag ccggtgtgtc ttccgtggcg tcgcgcccat tggatgacgg
     3901 cgcatccaga cgacggtcga aaccactgcc gctgccaagg ccacaagcgt gagcgtgatc
     3961 gcagcccatg cgtaggcgtc cagatgccgc ggcagttgat aaaaacccgc aacgcccgca
     4021 cccatcacca acagcgacca ccagaacagg tgtttaccga acaccgcgtt caccgcgaac
     4081 cagcgcgtct cagaagccat ggtggagggc agcctcacgc cgtacaccgg attgatcggc
     4141 accagacgga gccacagtgg caatgcgagc acgaagaaca gcagacccat gccgcagatg
     4201 actgtcggga tgataatgtt cgccattcca cccggcacgg cagtgctcca tttcccaacg
     4261 agccagaaca gcatgccgca aaacagggtg gtggccggaa tcgtccagaa tatccgcgat
     4321 gagctgccgg cctgctcgcg tgtggtcccg ccgcactcca cttgaagctg tcgccgcgcc
     4381 caccagggca ccagcgtcat gagcagtccc aaaggcagcc acagcaggtt cagcgccgtc
     4441 aaatacggac tggagcgaat catccattgc agagcaggag gcagctcgga aatgcttttc
     4501 acgacggttc ccgcgcccac cacgccgccc atgccgtgat tgcccgccgc gacaccgcca
     4561 tgcgcgtaaa caccgacggc cacaccgccg cacgccatcc aacccatcgc gactcctccc
     4621 atcgcgagag caccgacggc cacgccgcca acggcgagca gcagcgccac agccaaacct
     4681 ccaatcgaaa tcaaccccgt gctcagtccg ccaaagctca ccactcccac ggccagacca
     4741 ccgcaggcga aaaaaccgcg cgcaatgccg ccgaaggcaa agacgccctt tgccagatcg
     4801 ccaaacgcga agaagccatg cgcctgccgt cgtttgcccg tcgcaggatc gcgcccatgc
     4861 acgacgtgca gcagcggcag cccccaaagc gtgcgcctgg acttgtattc gaacgcgtcc
     4921 cagcgattcc cgcgaaccgc cgcacggcct ggcgcggcat gcgcgccttc gacctgcgtc
     4981 ttgaactcgc taacgctctg ctgccggcgc gtgcgttcct tctccaacgc gcgaaacacg
     5041 atctcgtcga tgtttccgcc cacggcggcg atctcggaag gcgcggcgaa gcggcccagc
     5101 ggcagctcac cggtgagcat ctcgtaaaac acgacgccaa ggctgtagat gtccgcgcgg
     5161 tgatcgacgc tggacggctg ctcgatctgc tccggggcca tgtagggcgc ggtgccgagt
     5221 tttgcgccgc tttgcgtgag cagcatcgcg tcaccacctt tttcatcgag gattttggca
     5281 atgccgaagt cggcgatctt cacgcggccc ttcgaatcga gcagaatgtt ctccggcttg
     5341 atgtcgcgat gcaaaacgcc ctgcgtgtgg gcgaactgaa gcgcgtcgca gatggccgga
     5401 atgatgcgca gcgcctgctc cggcgtgaaa cggcccgccc gcatggcctg gcggaggttc
     5461 acgccatcca cgaactccat gatgagatac gcgaagccgc cgctctcgcc aaaatcatgc
     5521 acggcgacga tgctcgggtg gttgagacgc gcgagcacgc ggccctcacg gttgaagcgc
     5581 tctgcaaagc ccggcgtggc cgcgagcgac tttggcagca ccttgagcgc gacaacgcgg
     5641 tcgagcttcg gctgccgcgc cttgaaaacc gcgctcatgc cgccatgacc gatgagtccg
     5701 aggatttcga gatcgggaaa cgcgacggca atttcctcca gcgagggcag atcggcgcgc
     5761 tgaagcggga tcgtgtcggc accgccatcc gtcggcatcg cggcggcggc catgagatcg
     5821 gcggggttca ggccgtggaa gggatcggaa ggtgaggagt tcatgtcacc ggctgcatgg
     5881 ggaatgcggc ggcggaggtt acacgaattt tctcccagcg acgctttttg tcaccgcgtg
     5941 agcaattcct caccgcgata aatcacacgc tcgaccatgc cctggagcgt ctggtcgagg
     6001 aagggcgtgt tgacgctctt cgacttcatg aactcgcgcg tgaaggtggt ggtgcgggcg
     6061 gggttgaaga tgatgaactc ggcgatgccg ccttcttgga tcggaacggg cgggagtttg
     6121 aagaggcggc gcggttcggc gctgtagcgc ttcacgatga gtctccagtc gaggatgccc
     6181 ttgctgatga agcggtcgta gagcgagacg agggcggttt cgagaccggt gataccgaag
     6241 ggggcgctgg cgaaatcctg gttcttctca aagggcgtgt gcggggcgtg atcggtggcg
     6301 atgacgtcaa accagccatc cttgagaccc tggaggagcg cggcgttgtc ctccggagtg
     6361 cgcaggggtg gattcatttt gtagtgcgta tcgtaatcgc caatgtcctc gtggttgaac
     6421 atcaggtggt gcggcgcgat ctcgcaggtg acgcgaatcc cgcggtcctt ggcgcgcttg
     6481 atggtgcgca tgccctccgc agtggtgacg tgctggatgt tcaggtggac gccggtgaac
     6541 tcggccaggc gaatgtcgcg cgagatgcag atttcctcac tgatgctggg aatgcccggc
     6601 aaaccaaggg cgtagctgat tttgccctca tgcatgcagc cttttcccga gagctccttg
     6661 acctcgcaat ggctggcgag aggcaggtcg aactcgcgcg catattccat ggcccggcgc
     6721 agcacttggg gattgtccac cgggaaaccg tcatccgtga gcaggaccgc tccggcggcc
     6781 ttcatgccgg cgatgccggc gagttcctcg cctttgcggc ccttggtaat ggcgccgctg
     6841 gtgaagatgc cgggaccgat gcgtgtgcgg cgggcgcttt cgagcacgct gcgcacgacg
     6901 ccggcgttgt cgatggcagg cgaggtgttc ggcatcatga tgaaacctgt gacaccgccg
     6961 ttgatggccg cctccgcgca ggtggcgatg ttttccttgt cctcctgccc cggttcgcgc
     7021 gcatgaacat ggatgtcaaa catggccggc ataagcacgc gccccgcgca gtcaatgacc
     7081 tccgcgtcat ccgcgccggt gatgccatgc gccacgcgca ggatgcgctc cccctcaatc
     7141 agcacatcgg cggtctgaag ttgcgcggag tcttcgttgg cgatctgggc ttggcggtag
     7201 aggcgtttca ttgcggcggc atgatgacgc gcggcgcggc ggtggcaaga agggcgttac
     7261 cgctgcgcgc caggccagca tgtcgaaagc gcaggctcag accaaagaga gcgcattggc
     7321 ctggagcagg gttttatccc accatgcggg tctgaaactc aaacgacgcc ggctccttca
     7381 tgcggcccta atcctcccgg ccgcaggctg cgccaccccc gcaccttcca aaaccatgaa
     7441 actcaccctt cgcaaatcca acgaacgcgg ccacgccgac cacggctggc tcgacagcta
     7501 tcacaccttc tccttcgccg attactacga tccggcgcac atgggcttcc gcagcctgcg
     7561 ggtcatcaat caggacatca tcgctcccgg cgcgggcttc cccacgcatc cgcacgcgga
     7621 catggagatc ttcagctaca tcctctgggg ggcgctggcg cacaaggaca gcatgggcaa
     7681 tggccgcacg ctgctgcccg gccagattca gctcatgagc gccggtcgcg gtgtgacgca
     7741 cagcgaattc aatccctcga agaccgaagg cgcgtcgctg ctgcaaatct ggatccgccc
     7801 gcatcagcgt ggcctcacgc cgagctacac cgaatggcat cccgattcca cgaaggaaaa
     7861 cgagccgaaa gtgctcgtca tctcgcccga tggccgcgaa ggcagcgcca ccattcatca
     7921 agacgccgac atctaccgcg tgcgccttcc gaagggccag a
//
LOCUS       DAOMYJ010000232         7922 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Prosthecobacter sp. isolate AMR_MDS_5629
            NODE_3039_length_7922_cov_21.733952, whole genome shotgun sequence.
ACCESSION   DAOMYJ010000232 DAOMYJ010000000
VERSION     DAOMYJ010000232.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867850
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prosthecobacter sp. (wastewater metagenome)
  ORGANISM  Prosthecobacter sp.
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Verrucomicrobiaceae; Prosthecobacter.
REFERENCE   1  (bases 1 to 7922)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7922)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.30
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:34:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,742
            CDSs (total)                      :: 4,684
            Genes (coding)                    :: 4,676
            CDSs (with protein)               :: 4,676
            Genes (RNA)                       :: 58
            tRNAs                             :: 55
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7922
                     /organism="Prosthecobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3039_length_7922_cov_21.733952"
                     /isolate="AMR_MDS_5629"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1965333"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..185)
                     /locus_tag="PLP58_18410"
     CDS             complement(<1..185)
                     /locus_tag="PLP58_18410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ10561.1"
                     /translation="MSGISTLDASETLRPPGHRPVESGVHALTGARVVTEPGRELENA
                     VIILRDGRVAAVGPGAPV"
     gene            complement(314..2299)
                     /locus_tag="PLP58_18415"
     CDS             complement(314..2299)
                     /locus_tag="PLP58_18415"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HRJ10562.1"
                     /translation="MADTNSKPASTATRSRKTPGAKLAGMNALALLEGAMGGGSTDWE
                     PPEAAELEPDFKGYRDFQFIDRGGMGAVYSAVQESLERRVAVKILPPEMALDAAFVER
                     FNQEARLLAKLQHPHIVAVYDFGSTAAGHLFIVMEYVEGESLLEVMKKRRLPVREVLD
                     IIAQVCDALQFAHDRGVIHRDIKPTNILMDEKGAVRVADFGLAKLAAATPPTTSTRTG
                     MIMGTPGYAAPEQRRADTLVDHRADIFSTGATLYEMLTGHLPVGVFVPPSKKSGAPPS
                     VDKIVFKALQELPADRFQRASDMRAAIATVMLRLGTPLVKHTIITRPMVSMMSCVIIG
                     MGFIYLLDAINNELRGTRAPLHAALTTELAVSDAMLVQLDAEYAVVRARFSWEDASRH
                     IDDLAGWKMAEFHSDEQRLAVAGLLKERGITSPLWIGGYETENGGHAWISGAPFDHAA
                     WMPAAPEPPPIITEIQPKNETTLVLPSGTSPDWFEVFNPGTLPVDLTGYHLKHYTSRV
                     VYDGRLGPAVVKDPESLVLQPGGRLVVYCHSDIAAQVREPCFPFHLSAPSGRVVWLDP
                     RGRVIQNFEQNWQDFPADASLGCDDDGTRWGWRAKATPGAPNSPLEALSHQPVDMINQ
                     PRAILMLPEFDCRWTLNPTRRGTWSLVRRVNEPRAAN"
     gene            2412..3146
                     /locus_tag="PLP58_18420"
     CDS             2412..3146
                     /locus_tag="PLP58_18420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HRJ10563.1"
                     /translation="MRTQHGAFPATRWSVVVASRNSADAAANRMALDELCGIYWHPLY
                     SYARHQGIAPADAEDLTQSFLVFVLEKDLFAQADEALGRLRNYLLTAFGRHIKHWQRQ
                     AGALKRGGGQQIVSIEASQAEDNVTIDPADHRTPEEVYQRQCALSMIETAVCHLAAEQ
                     DAAGKGGQFEMLRPRLDPTQSCSGNDAELAAALGMSHDAVRKTISRLRKRFREILHEL
                     VAATLSNPTEESIQEELASLRNALVG"
     gene            3277..3588
                     /locus_tag="PLP58_18425"
     CDS             3277..3588
                     /locus_tag="PLP58_18425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ10564.1"
                     /translation="MSTAISLSIFGARILHHTMPLAAGTDEFVQQFSGVVNILNLVGL
                     VLAFGGLLFAAVMFMMGQTERVLYGLVGAGIGGLSFLIVKVMFSTAGGSGDVDNIQMG
                     Q"
     gene            3684..4055
                     /locus_tag="PLP58_18430"
     CDS             3684..4055
                     /locus_tag="PLP58_18430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ10565.1"
                     /translation="MDEQTVRFHETNEGRETQQGIAGFEGLNALFFMGALGLAMILFR
                     RFSEGPGASMSMAALIASIPLVVVSAYVFGLKQGKPKSYDVELGEWLIIKFSKAPYFS
                     PRQVEPLALPWVEDAKKKEDV"
     gene            4059..6752
                     /locus_tag="PLP58_18435"
     CDS             4059..6752
                     /locus_tag="PLP58_18435"
                     /inference="COORDINATES: protein motif:HMM:NF040179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ10566.1"
                     /translation="MAKFVNTYLDEDLIIYRGLQRGGAIGKGYNVEMPDTENTDAPWL
                     MALEDNIRVLLRLIRPELRLQVAWSVDSDYRKELERYKSKTEELGCDKWSLRAREERY
                     RRYDRKIRNHMLRREHLQYFFTSRIQGRAMGGGRQYYEELLLAAKREQAELEEALRQQ
                     LGSLGGRVSPMTDMEHFETFYRYFNPSAFENEALKLEDIYDPTKSVCEMCFNSDAAPV
                     KLGASTFKMDGFYQGICVMKTLPKFTYIGMMRLLTQLDILDYQVVVNIEAGDVEKDRV
                     ATEAKVARLERGKITPSLEATLIKLRTRIRRLTTNEVVPYRMHLLVRVWDRDEATCAS
                     KLSAIKNSILKLGGAQAYEPTLPTSVRNYWQLCMPGWTWANYNDFKLYVEDVNLADLL
                     PISSTPVGDLEDAEAIYDGPRGALVGCVTFVGKKGAQRPEHGIMFGASGAGKSVFTID
                     LLTQTAPYFDYTAIVEEGLSYNLFTRLYGCTPIIVQPNGNLTFNYFDTRGLPLSPDQL
                     GDATAVAHLMAGPPPDTDKDRIRQALLAKQVERLYVDQFETWANRNADLRVEAARRFA
                     VADEWRRRKMPQGTVISDAWLDFHSAYQEGETEANDLFGMSLDEARLEDFLNDPVNQW
                     DHMSMGYTLMKPEDMPTHSQFVELLNLESRQRRAHEDLGLLRMLLEPWSRTGRYGAIM
                     DGVNNVNLKGKVVHFELSYIPESAKELRTVAAFLITNDLRKEIMSRPRSTRKRVILEE
                     LSAFLAMPDGDRITREYYERMRKYSCWVFSIIQQFQRIKDHPVRSSVVGNSRVMFMLK
                     QPDQRDVQSMSEGFRIPEVTKRQVTSFPDPSEMKNDPFASFVYYHEATGRPRIAIGRH
                     QASKEMILASATSGEAFERQNQELKEYGGDAYKMILSQAKT"
     gene            6749..7267
                     /locus_tag="PLP58_18440"
     CDS             6749..7267
                     /locus_tag="PLP58_18440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ10567.1"
                     /translation="MKTHHLILAALCAQMAACTSSDGTANKGLLGAATGGVIGALLGD
                     GALSRNNNNNNAAGGGAGGGAGGGNQGGNDGDFLDEHSGLITGAAAGFAAGAITDAFA
                     KNEVRQKYIEGYNKGRSDSIKELYWLKRDAERPPEESLVQRRYYEVPVPEHITTDGVL
                     VAPHKRVIEVVE"
     gene            7287..>7922
                     /locus_tag="PLP58_18445"
     CDS             7287..>7922
                     /locus_tag="PLP58_18445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ10568.1"
                     /translation="MKTRHQRQAEIRNSKYETWFIPVIAILLAPAALHAQAEAGDAAI
                     IEVLMKNHSELMPMLQKQLEEAERQASKLEQQLERMGDPGAVNPESIAMIKDDVKRSA
                     SVLMTKQEKREMYDSLTGAEVFDDDANGMLEAIGAKVTLEDGSEQDRDAEKYKLEAAL
                     MAEIKQYKEVREQAIEQQKMLSDELVTVMEELEAAKDFATVQKLNATISIIN"
BASE COUNT         1568 a         2549 c         2454 g         1351 t
ORIGIN      
        1 acgggcgcgc cggggccgac ggcggcgacg cgcccgtcgc gcaaaatgat gacggcgttt
       61 tccagctccc ggcccggctc ggtgacgaca cgcgcgccgg tgagcgcgtg aaccccggac
      121 tccacaggcc ggtgccccgg cgggcggagg gtctccgacg cgtcgagggt ggagatgcct
      181 gacacggcgg gtagcaaaca gaaataaatg aatttcatcc tgccgcgcat cctgagggta
      241 tgcgctcagc ttggcaagaa gttgcatgat tgcctgcgcg gagatttccc ggaggcaggc
      301 cggccagccc gtctcagttc gctgcgcgcg gttcgttgac acgccgcaca agggaccagg
      361 tcccccggcg ggtggggttc agtgtccagc ggcagtcgaa ttcgggcagc atgaggatgg
      421 cgcgcggctg gttgatcatg tccacaggct gatgactcag cgcctccagc gggctgttgg
      481 gagcgccggg ggtggctttg gcgcgccagc cccagcgggt gccgtcgtca tcgcagccca
      541 gggaggcgtc ggcgggaaag tcctgccagt tttgctcgaa attctggatg accctgccgc
      601 gcgggtcgag ccaaacgacg cgccctgatg gcgccgagag gtggaagggg aagcagggtt
      661 cccggacttg cgccgcgatg tcgctgtgac aatagacgac gaggcgtcca cccggctgga
      721 gaacgaggct ttcggggtct ttgacaacgg cgggaccgag cctgccgtca tagaccacgc
      781 ggctggtgta gtgcttcaaa tggtagcctg tgaggtccac aggcagcgtg cctggattga
      841 agacttcaaa ccagtcgggg ctggtgccgg agggcaggac aagggtcgtc tcgtttttgg
      901 gctggatttc ggtgatgatc ggcggcggct ccggcgccgc gggcatccat gccgcgtggt
      961 cgaagggcgc gccgctgatc caggcgtggc cgccgttttc ggtttcatat ccgccgatcc
     1021 agagcgggct ggtgatgcct cgttctttga gcaggccagc cacggcaagg cgctgctcat
     1081 cggagtggaa ttcggccatt ttccagcccg ccaggtcgtc aatgtgacgg ctggcatcct
     1141 cccagctgaa ccgcgcgcgg acgacggcat attcagcatc cagctgcacg agcatggcgt
     1201 ctgagacggc gagctcggtg gtcaatgccg cgtgaagcgg ggcgcgggtt cccctcagct
     1261 cgttgttgat ggcgtcgagc aggtagatga agcccatgcc gatgatgacg caactcatca
     1321 tggagaccat ggggcgggtg atgatggtgt gcttcaccag cggcgtgccg agccgcagca
     1381 tgacggtggc gatggcggcg cgcatgtccg aggcgcgctg gaagcggtcc gccggaagct
     1441 cctggagcgc cttgaagacg attttgtcca cagacggcgg agcgccggac tttttcgagg
     1501 gaggcacgaa aacacccaca ggcaggtggc cggtgagcat ttcataaagc gtggccccgg
     1561 tgctgaagat gtccgcccga tggtcaacca gtgtgtcggc gcggcgctgc tcgggagcgg
     1621 catagccggg cgtgcccatg atcatgcctg tgcgcgtgct ggtggtgggc ggggtggcgg
     1681 ccgcgagctt ggccaggccg aagtcggcca cccgcaccgc gcccttttca tccatcaaaa
     1741 tattggtcgg cttgatgtcg cggtgaatga ctccgcggtc gtgggcgaac tggagggcgt
     1801 cgcagacctg ggcgatgatg tcgaggactt ccctgacagg cagccggcgc tttttcatca
     1861 cctccagcag gctctccccc tcgacatact ccatgacgat gaaaagatgc cccgcggcgg
     1921 tggagccgaa gtcatagacg gccacgatgt gggggtgctg aagtttggcc agcaggcgcg
     1981 cctcctggtt gaacctttcc acaaaggcgg cgtccagggc catttctgga gggaggattt
     2041 tcaccgccac gcgccgctcc agggactcct gcacagccga gtaaaccgcg cccatgccgc
     2101 cgcggtcaat gaactgaaaa tcgcggtagc ctttgaaatc cggctccagc tcggcggctt
     2161 cgggcggctc ccagtccgtg ctcccgcccc ccatcgcgcc ctccagcagg gccagggcgt
     2221 tcatgcccgc gagtttggcg cccggagtct tcctgctccg ggtggctgtg ctggcgggtt
     2281 tggaattcgt gtcagccatg accgccgtgt tttacaccat gagcgcggga ttgtgactcg
     2341 tttttttccg cgttgaagca taatccaggc ttgcaatccc gtgcttggaa ggaatttcat
     2401 cacccttcgt catgcgcaca caacatggag ccttccccgc cacgcgctgg agcgtcgtcg
     2461 tcgcgtctcg gaacagcgcg gacgcggcag ccaaccgcat ggctttggat gagttgtgcg
     2521 ggatttactg gcacccgctg tactcttatg cgcgccacca ggggatcgcg ccggcggacg
     2581 cggaggacct gacccagagc tttttggtct tcgtgttgga aaaggacctt tttgcccagg
     2641 cggacgaggc gctggggcgg ctgcgcaact acctgctcac cgcctttggc cgccacatca
     2701 agcactggca gcgccaggcg ggggctctca agcgcggagg cgggcagcaa attgtctcga
     2761 tcgaggccag tcaggcggag gacaatgtca ccattgaccc tgctgaccac aggacaccgg
     2821 aggaggttta tcaaaggcag tgcgcgctga gcatgatcga gaccgccgtc tgccatctcg
     2881 ccgctgagca ggatgcggcg gggaagggcg gccagttcga gatgctgcga ccgcggctcg
     2941 atcccaccca gagctgctcc ggcaacgacg ccgaactggc cgccgcgctg ggcatgagcc
     3001 atgacgcggt gcgcaagacc atctccaggc tcagaaaacg gttccgcgag atcctgcacg
     3061 aactggtcgc cgccactttg agcaacccga cggaggagag cattcaggag gagctggcct
     3121 ccctgcgcaa cgcgctggtg ggctaggaaa gcgcggcggg atgaggattt tccaagtccg
     3181 gaaaaaaagt ttgtcacact gaggcgggcg tcaggctaac aacgccatca cacggtgccg
     3241 tccaggcgcc gggaacccac aaacacacac atccttatgt ccacagccat ctccctctcc
     3301 atcttcggag cgcgcatcct tcatcacacc atgcccctcg ccgctggcac tgatgaattc
     3361 gtccagcagt tcagcggggt cgtcaacatc ctcaacctcg tcggcctggt gctcgccttc
     3421 ggcgggctgc tcttcgccgc cgtcatgttc atgatgggcc agaccgagcg cgtgctttac
     3481 ggcctggtcg gtgccggcat cggcggcctg tccttcctca tcgtcaaggt catgttctcg
     3541 accgccggcg gttccggtga cgtggacaac attcagatgg gccagtaagc gcccgatgcg
     3601 tccgttgatt tcccggcccc gcccgccgcg cggggttcgg gaagtccccg gtttcatccc
     3661 gaactccggc ggcatatccc ttcatggacg agcaaactgt cagattccac gaaaccaacg
     3721 aaggacgcga gacccagcag ggcatcgcgg gatttgaggg cctcaacgcc ctgttcttca
     3781 tgggggccct tggactggcc atgatcctct tccgccgttt ttccgagggg cctggcgcca
     3841 gcatgagcat ggcggctctc atcgcctcca tcccgctcgt cgtggtctca gcgtatgtct
     3901 tcggactcaa gcagggcaaa cccaaatcct acgatgtgga gctgggcgag tggctcatca
     3961 tcaaattttc caaagcaccc tatttttccc cccgccaggt ggagccgctg gccctgccct
     4021 gggtggagga tgctaaaaag aaggaggatg tctgacgcat ggccaagttc gtcaacacct
     4081 acctcgacga ggatctcatc atctaccgcg gtcttcagcg cggcggggcg atcggcaagg
     4141 gctacaacgt cgagatgccc gacaccgaga acacggacgc gccgtggctc atggccctcg
     4201 aggacaacat ccgcgtcctg ctccgcctca tccgccccga gctgcgcctg caggtggcct
     4261 ggagtgtgga cagcgactac cgcaaggagc tggagcgcta caaaagcaag accgaggaac
     4321 tcggatgcga caaatggtcc ctccgcgcgc gcgaggagcg ttaccgccgc tacgaccgca
     4381 aaatccgcaa ccacatgctg cggcgcgagc atctgcagta tttcttcacc tcacgcatcc
     4441 agggccgcgc catgggcgga ggccggcagt attacgagga gctgctgctg gcggccaagc
     4501 gcgagcaggc cgaactggag gaggctctgc gccagcagtt gggcagtctt ggcgggcgtg
     4561 tctcgcccat gactgacatg gagcacttcg agacctttta ccgctacttc aaccccagcg
     4621 cctttgaaaa cgaggcgctc aaactggagg acatctacga tcccaccaag tccgtctgcg
     4681 agatgtgctt caacagcgac gccgcgccgg tgaagctggg cgcgtccact ttcaaaatgg
     4741 acggctttta ccagggaatc tgcgtcatga agacgctccc caagttcacc tacatcggca
     4801 tgatgcggct gctcacccag ctcgacatcc tggattacca ggtcgtggtg aacattgagg
     4861 ccggggatgt cgagaaggac cgtgtggcca ccgaggccaa ggtggcccgc cttgagcgag
     4921 gcaagatcac gccctcgctg gaggccacgc tcatcaagct gcgcacccgc atccgccgcc
     4981 tcaccaccaa cgaggtcgtg ccctaccgca tgcacctgct cgtgcgcgtc tgggaccggg
     5041 acgaggccac ctgcgccagc aagctctccg ccatcaaaaa ctccatcctc aagctcggcg
     5101 gcgcgcaggc ttacgagccg acgctgccca ccagcgtgcg caactactgg cagctttgca
     5161 tgcccggctg gacatgggcc aactacaacg acttcaagct ctacgtcgag gacgtgaacc
     5221 tcgccgacct gctacccatc tccagcacgc ccgtgggcga cttggaggac gccgaggcca
     5281 tctatgacgg gccgcgcggc gcgctcgtgg gctgcgtcac ctttgtcggc aaaaaaggcg
     5341 cgcagcggcc cgagcatggc atcatgtttg gggcctcggg cgcgggcaag tccgtcttca
     5401 ccattgacct gctcacccag accgcgcctt attttgacta cacggccatc gtcgaggagg
     5461 gcctctccta caacctgttc acccgcctct acggctgcac tcccatcatt gtgcagccga
     5521 atggcaacct caccttcaac tacttcgaca cccgcggcct gccgctcagc ccggaccaac
     5581 tcggggacgc caccgcggtc gcgcacttga tggccggccc gccgcccgac acggacaagg
     5641 accgcatccg ccaggccctg ctcgccaagc aggtcgagcg gctttatgtg gaccagttcg
     5701 agacctgggc taaccgcaac gccgatctgc gcgtcgaggc cgcccgccgc ttcgccgtcg
     5761 cggatgaatg gcggcggcgc aagatgcccc agggcaccgt catttccgac gcctggctcg
     5821 atttccacag cgcttaccag gagggcgaga ccgaggccaa cgacctcttc ggcatgagcc
     5881 tggatgaggc caggctggag gacttcctca acgaccccgt caaccagtgg gaccacatga
     5941 gcatgggcta caccctcatg aaacccgagg acatgcccac gcacagccag ttcgtcgagc
     6001 tgctgaacct ggaaagccgc cagcgccgcg cccatgagga cctgggcctg ctgcgcatgc
     6061 ttctggaacc gtggagccgc accggccggt acggagccat catggacggt gtcaacaatg
     6121 tgaacctcaa gggcaaggtc gttcatttcg agctttccta catccccgag tccgccaagg
     6181 agctgcgcac cgtggccgcc ttcctcatca ccaacgacct gcgcaaggag atcatgagcc
     6241 gcccgcgcag cacccgcaag cgcgtcatcc tggaggagct ttccgccttc ctcgccatgc
     6301 cggacgggga ccgcatcacg cgcgagtact acgagcgcat gagaaagtac tcctgctggg
     6361 tcttctcgat cattcagcag ttccagcgca tcaaggacca ccccgtgcgc tcctccgtgg
     6421 tgggcaacag ccgcgtcatg ttcatgctca agcagcccga ccagcgggat gtgcagagca
     6481 tgtccgaggg cttccgcatc cccgaggtca ccaagcgcca ggtcaccagc ttcccggacc
     6541 cctcggaaat gaagaatgac ccctttgcct ccttcgtgta ttatcacgag gccactggac
     6601 gcccgcgcat cgccatcggc aggcaccagg cgtccaagga aatgattctg gccagcgcca
     6661 cctccggcga ggcctttgaa agacagaacc aggaactcaa agaatacggc ggagacgcct
     6721 acaagatgat cctcagccag gccaaaacat gaaaacgcat cacctcatcc tcgccgcgct
     6781 ctgcgcccag atggccgcat gcaccagcag tgacggcacc gccaacaagg gactcctcgg
     6841 cgcggccacc ggcggcgtca tcggcgcgct cctcggagat ggcgccctca gccgcaacaa
     6901 taacaacaac aacgcggcag gcggcggcgc gggcggcggg gctggaggcg gcaaccaggg
     6961 cggcaatgac ggagactttc tcgacgagca ctccggcctc atcaccggcg ccgcggccgg
     7021 attcgccgca ggcgccatca ccgacgcctt cgcaaaaaac gaggtgcgcc agaaatacat
     7081 cgaaggttac aacaagggcc gcagcgactc gatcaaggag ctctactggc tcaagcgcga
     7141 cgccgagcgc ccgccggagg aaagcctcgt acagcgccgc tattatgaag tccccgttcc
     7201 cgagcatatc accaccgacg gcgtgctcgt ggccccgcac aagcgggtga tcgaagtcgt
     7261 cgaatgaacc cgaaccacag cccgtcatga aaacgagaca ccaacgccag gcagaaattc
     7321 gaaattcgaa atacgaaaca tggtttatac ctgtcatcgc gattttgctc gcgcccgccg
     7381 cccttcatgc ccaggcagag gccggggacg cggcaattat tgaggtgctg atgaaaaacc
     7441 acagcgagct catgcccatg ctgcaaaagc agctcgaaga ggccgagagg caggcatcca
     7501 aacttgagca gcaacttgaa cgcatgggag accccggcgc ggtcaacccc gaatccatcg
     7561 ccatgatcaa ggacgatgtg aaacgctccg caagcgtgct catgaccaag caggaaaaac
     7621 gcgagatgta cgattccctg accggggccg aggtctttga tgatgacgcc aacggcatgc
     7681 tggaggccat tggcgcaaaa gtcacccttg aagacggttc agagcaggat cgtgacgcgg
     7741 aaaagtacaa actggaagcc gctttgatgg ctgagatcaa gcaatacaag gaagtccggg
     7801 agcaagccat cgaacagcaa aaaatgctct ccgacgagct ggtcacagtc atggaggaat
     7861 tggaggccgc caaggacttc gccaccgtgc aaaagctcaa cgccaccatc agcatcatca
     7921 ac
//
LOCUS       DAOMYJ010000233         7815 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Prosthecobacter sp. isolate AMR_MDS_5629
            NODE_3091_length_7815_cov_23.788273, whole genome shotgun sequence.
ACCESSION   DAOMYJ010000233 DAOMYJ010000000
VERSION     DAOMYJ010000233.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867850
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prosthecobacter sp. (wastewater metagenome)
  ORGANISM  Prosthecobacter sp.
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Verrucomicrobiaceae; Prosthecobacter.
REFERENCE   1  (bases 1 to 7815)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7815)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.30
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:34:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,742
            CDSs (total)                      :: 4,684
            Genes (coding)                    :: 4,676
            CDSs (with protein)               :: 4,676
            Genes (RNA)                       :: 58
            tRNAs                             :: 55
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7815
                     /organism="Prosthecobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3091_length_7815_cov_23.788273"
                     /isolate="AMR_MDS_5629"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1965333"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..267
                     /locus_tag="PLP58_18450"
     CDS             <1..267
                     /locus_tag="PLP58_18450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018970528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HRJ10569.1"
                     /translation="QAFRRVALPAARRGMVAAFCISWARSLGEFGPILVFAGATRMRT
                     EVLPTTVWLELSVGNLEAAVAVSLLMILIAVAVLVIVRITGERV"
     gene            264..914
                     /locus_tag="PLP58_18455"
     CDS             264..914
                     /locus_tag="PLP58_18455"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRJ10570.1"
                     /translation="MIRLENISWRAGAFDMRGICLEVPSRGYGVLMGRTGCGKTTLLE
                     ILCGLRPPLEGRVWVDGVDVSDLPPRQRGIGYLPQDIALFPTLSVRRQISFALDIRRR
                     PEKEIRERVGQLADELGITHLLDRQPDRLSGGEKQRVALGRALAAEPKVLLLDEPLSA
                     LDEDVRGDMTSLLKRVQKDHAITVLHVTHSRAEAQALADVILRLQPGGIEIEKTAA"
     gene            911..1678
                     /locus_tag="PLP58_18460"
     CDS             911..1678
                     /locus_tag="PLP58_18460"
                     /inference="COORDINATES: protein motif:HMM:NF013094.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008641 - ubiquitin-like modifier
                     activating enzyme activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HesA/MoeB/ThiF family protein"
                     /protein_id="HRJ10571.1"
                     /translation="MIQNDTPMPELSDEERARYEWQMWHEGMGDEGQRKLKAATVLIS
                     RVGGLGGLVAYELAAAGVGRLILAHAGALKPADLNRQLLQTTANLGRSRMESIIRRLR
                     ALNPHVRLTGIPENISQDTADALVSQADIIVDAAPLFEERLALNDAAVRRRRPMVECA
                     MRGMEATVTTFIPGRTGCLRCYVPGVPPSWKRQFPVFGAVSGTAACIGATEVIKLITG
                     IGELLAGVMLSMDLATMSFRKTRLPRNPDCVCCGCAA"
     gene            1715..3280
                     /locus_tag="PLP58_18465"
     CDS             1715..3280
                     /locus_tag="PLP58_18465"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF019581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047420 - N-acyl-D-amino-acid deacylase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacylase"
                     /protein_id="HRJ10572.1"
                     /translation="MRPLLILPLLALSAHAADFDVLIQNARIADGTGAPLVTGGIGVK
                     DGRITAVGGVTGSADTVIDAEGRVVAPGFVDVHTHSEKICENPVVENFLRMGVTTIIT
                     GNCGNSKTDVAAFFDEIGKTGTTLNVATLIGHGSVREQGMGGRFIRPPNAEQLDAMKK
                     LVDQAMRDGAVGMSTGLIYVPGSFARTEEIIELAKVVSAHGGVYASHMRYETDRIFEA
                     LEELARIAREARIRAEVSHIKLSGPSAWGRAREVLAWLDKARAEGLEITHDQYAYPAS
                     STGLRQTIPDSALEGTREDYAARLDDPGQKGLIIERMKKMLSDRGFKDYSYAVIARYQ
                     KQPELNGLNIREAALKVRGGDSLDDQIELLLDIERQGGGSAVYHSMNEDDLKVFLAHP
                     LTMIASDGGPRRLGEDIPHPRSYGNNARVLGRYVRDLKALTLEDAIRRMSSLPARTFH
                     LHQRGEIRQGYWADLVIFDPDTVSDPSSFDAPHKYATGFSDVLVNGVPVIRAGQLTEA
                     RPGGPLRKAQPAP"
     gene            3408..4175
                     /locus_tag="PLP58_18470"
     CDS             3408..4175
                     /locus_tag="PLP58_18470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ10573.1"
                     /translation="MKPLLLSFLVSALSLGAADPAPADSVKTKPLRSYSDFLAAIPKD
                     MEPWDARNWTAAQKEVANGILKKKLVDAKRPVRMRFKVHGVDYWGRFVLWSHLPADEG
                     YAIRIFASQWREADMLPKLAALRKGDLIEMTGLCDLAKFENLWNTDSLSLGIGNAAFI
                     KLQPDGSPAPGPERVPVKLVSAVYGSGTQFTDVTGRVKALLDEPGAVFHANPHWLGAD
                     PTPGWNKALVIVHENRGVRSVFTTGENGEVSAALVVK"
     gene            4372..5364
                     /locus_tag="PLP58_18475"
     CDS             4372..5364
                     /locus_tag="PLP58_18475"
                     /inference="COORDINATES: protein motif:HMM:NF024772.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HRJ10574.1"
                     /translation="MKTTGQHRLLTPGNLLVVALSAGMWAAAFMLPVPVPAQPLRVAV
                     PLWPGAEPWVMARAEGKLRPDQIKLVEINWTSAAMRAVGNRVVDAAILSLEEALLQIH
                     QGYPLKILMVTDVSRGADALLVNPGIESLADLRGRRIGFEPRTAGTRLLGLALAEAGL
                     SMADIRHVVMNATELQDGGMDRPAEAVVCSEPWRQRLVARGMRPLYDSGKKGAEVVRV
                     LVAHVDAVADHRDELLVLVRAHVEGMPRLAAAGAELEPVLRREGVSAEEFARIMEKME
                     VPALEQNRRWLACEDGWLESRLEQLRAELLPADGLEPARRAADVFDPWFLEEAR"
     gene            5361..7118
                     /locus_tag="PLP58_18480"
     CDS             5361..7118
                     /locus_tag="PLP58_18480"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HRJ10575.1"
                     /translation="MRRALTSLQFKVSASIFLMGAVLITLSAARMTSRAIESRHARVV
                     AHASMEGGRLAGMAQHLIRRQAPHVMDLELSYASAQQDLELGLILDDTGRVRLSTRQD
                     LRGLPLAETPMAEAAELVDQARFSMEGRVVEAEAGTKMRAVFPFREGLNERRGCVLLD
                     FDLERPLDEGMAAALHSSMVQGLALAGGCLVLWLLLKVVVTERVSMLVRQVEDISLES
                     DPGAPLEGADEISRVSQAASRTHARLRQSEQRLRQVAATMRDVFWLAPAEAAQEPYVN
                     PAYQSVLKRDPARLAARRWDWLHALAREDRRPCLERLRRLRSGEVVPEFEARVVSSPG
                     QTIWLRCRGFSVPGHAGAGRMVGGIAMDVSERKMLDRRLLDTAEKERQRVGVDLHDDL
                     CQRLAAVLMKTGILQAALERAESPQQALAAELARDLSEATALSRRFARGLAPVSIEAL
                     GLPAALADLGDFISRAFNIPCRVECTGAELNCGAETATHIFRVAQELANNAAKHGGCS
                     WIEIAFEAAAHEARLRIRHDGRPYDPQHSQGDGMGMHLVRQRLDALGASLGVICDEES
                     GVVQECRISLNDTPNTPTS"
     gene            7115..>7815
                     /locus_tag="PLP58_18485"
     CDS             7115..>7815
                     /locus_tag="PLP58_18485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009959953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HRJ10576.1"
                     /translation="MSSTPSPTPAARAHRVAVVDDHTLMREGMKMLLENLQGLSCAWT
                     AGDTSEALRLLEADMPDALVVDITMPGRNGLELIKDIRALYPDLAVLVVSMHDEALYA
                     QRALKAGAKGYVMKDAPHGVFEKAIRHVLAGGIALSEKMSESILLAFSSGTNPGPEGA
                     IHNLSDREFEVFQLIGEARSTAQIAAELGISPKTVDVHKLKIRQKLSLTESTSLTAFA
                     IRWVELRKLDIKEPA"
BASE COUNT         1371 a         2566 c         2559 g         1319 t
ORIGIN      
        1 caggccttcc gccgcgtggc gctgccggcg gcgcggcgcg gcatggtggc ggcgttttgc
       61 atctcctggg cgcgcagcct gggcgagttc ggccccatcc tcgtcttcgc cggagccacg
      121 cgcatgagga ccgaggtgct gcccaccacc gtctggctgg agctcagcgt gggaaatctg
      181 gaggccgccg tggccgtcag cctgctgatg atcctcatcg ccgtggccgt gctcgtcatc
      241 gtccgcatca caggagaacg cgtatgatcc ggctcgaaaa catctcctgg cgcgcgggcg
      301 ccttcgacat gcgcggcatc tgcctcgaag tgccgtcccg cggctacggc gtgctcatgg
      361 gccgcaccgg ctgcggcaag acgacccttc ttgaaatcct ctgcggcctg cgccccccgc
      421 tggagggccg cgtctgggtg gatggcgtgg atgtctccga ccttccgcct cgtcagcgcg
      481 gcatcggtta cctgccgcag gacatcgcgc tcttccccac actcagcgtg cggcggcaga
      541 tcagcttcgc cctggatatc cgccgccggc cggaaaagga aatccgcgag cgcgtcggcc
      601 agctcgctga cgagctcggc atcacccacc tgctcgaccg ccagccggac aggctctccg
      661 gcggcgaaaa gcagcgcgtg gctctcgggc gggcgctagc cgccgaaccg aaagttcttc
      721 tcctcgacga gcctctctcc gcgctcgacg aggacgtgcg cggcgatatg acatccctgc
      781 ttaagcgggt gcaaaaagac cacgccatca ccgttttgca cgtcacccac tccagagccg
      841 aagcgcaagc gctggccgat gtcatcctgc gcctgcagcc cggaggcatc gagatcgaaa
      901 aaaccgccgc atgatccaga acgacacgcc catgcccgaa ctcagtgatg aggagcgcgc
      961 ccgctacgag tggcagatgt ggcacgaggg catgggcgat gagggccagc gcaaactcaa
     1021 agccgccacc gtgctcatct cccgcgtcgg cggcctcggc ggcctcgtcg catatgaact
     1081 cgccgccgcc ggagtcggcc gcctcatcct cgcgcacgcg ggcgctttga agcctgcgga
     1141 tctcaaccgc cagctcctcc agaccacggc caaccttggc cgcagccgca tggaaagcat
     1201 catccgccgt ctgcgcgcgc tgaacccgca tgtgcgcctc accggcatcc cggaaaacat
     1261 cagccaggac actgcggacg cgctggtcag ccaggcggac atcatcgtgg acgccgcgcc
     1321 gctttttgaa gagcgcctgg ccttgaacga tgccgccgtt cgccgccgcc gccccatggt
     1381 ggagtgcgcc atgcgcggca tggaggccac cgtcaccact ttcattccgg gccgcaccgg
     1441 ctgcctgcgc tgttacgtgc ccggggtgcc gccgagttgg aagcgacagt tccccgtctt
     1501 cggcgccgtc tcaggcaccg ccgcctgcat cggcgccacg gaggtcatca agctcatcac
     1561 cggcatcggc gaactcctcg ccggcgtcat gctctccatg gacctcgcca ccatgagctt
     1621 ccgcaaaacc cgcctgccac gaaacccgga ctgcgtgtgc tgcggatgcg cggcttaata
     1681 ttcgaaattc gattttcccc aacgtttccc tcacatgcgc cctttgctca ttctcccact
     1741 gctcgctttg tccgcgcacg ccgccgactt tgatgtcctg atccagaatg cccgcatcgc
     1801 cgacggcaca ggcgcgcccc tagtcaccgg cggcatcggt gtcaaagacg gccgcatcac
     1861 cgcggtcggc ggcgtcaccg gcagcgcgga caccgtcatt gacgcggagg gccgcgttgt
     1921 cgcgccaggt tttgtggatg tgcacacgca ctcggaaaaa atctgcgaaa accccgtggt
     1981 ggagaatttc ctgcgcatgg gcgtcaccac catcatcacc gggaactgcg gcaactcaaa
     2041 aaccgatgtg gccgcctttt tcgacgagat cgggaagacg ggcacaactc tgaatgtcgc
     2101 caccctcatc ggccacggca gcgtgcgcga gcaaggcatg ggcggacgct tcatccgccc
     2161 gccgaacgcg gagcagctcg acgccatgaa aaagctcgtg gaccaggcca tgcgcgacgg
     2221 cgccgtgggc atgagcacag gcctcatcta tgtgccgggc agctttgcca ggaccgagga
     2281 gatcatcgaa ctggcgaaag tcgtttccgc ccacggcggc gtctatgcca gccacatgcg
     2341 ctacgagact gaccgcatct tcgaggccct ggaggagctg gcacgcatcg cccgcgaggc
     2401 gcgcatccgc gccgaggtct cccacatcaa gctctccggc cccagcgctt ggggccgcgc
     2461 gcgcgaggtg ctggcctggc tggacaaagc gcgcgccgag gggctggaga tcacccatga
     2521 tcaatatgcc tacccagcct ccagcaccgg cctgcgccag accatccccg acagcgccct
     2581 ggagggcacc cgcgaggact acgccgcgcg cctcgacgac cccggccaga agggcctcat
     2641 catcgagcgc atgaaaaaga tgctctccga ccgcggcttc aaggactaca gctacgctgt
     2701 cattgcccgc taccagaagc agcccgaact caacggcctc aacatccgtg aggcggctct
     2761 gaaagtgcgc ggcggcgaca gcctcgacga ccagatcgag ctcctcctcg acatcgagcg
     2821 ccagggcgga ggcagcgccg tctatcacag catgaatgag gacgacttga aagtcttcct
     2881 cgcccacccc ctcaccatga tcgccagcga cggcggaccc cgccgcctcg gcgaggacat
     2941 cccccacccg cgcagctacg gaaacaacgc ccgcgtgctt ggacgctacg tccgcgatct
     3001 caaagcactg acccttgagg acgccatccg ccgcatgagc agcctgcccg cgcgcacttt
     3061 tcatctgcac cagcgcggcg aaatccgcca gggttactgg gctgacctcg tcatatttga
     3121 tcccgacact gtcagcgatc cctccagctt tgacgccccc cacaaatacg ccaccggctt
     3181 cagcgacgtg cttgtcaatg gtgtgcccgt catccgtgcc ggccagctca ccgaagcccg
     3241 ccccggcggc cccctgcgca aggcccagcc cgcgccatga ggccgggaag cgaccgcttg
     3301 cgcggcgcca tcatcctttt gacgagttct ttacagcggc ggacattttc ctttcccaag
     3361 attcactgcc tgccattcgt gtctccgttt cgaatcttcc cacgctcatg aaacccctcc
     3421 tcctgtcctt cctggtttcc gctctttccc tgggcgcggc ggaccccgcg ccggctgatt
     3481 cggtgaaaac caagccgctg cggagctaca gcgacttcct cgccgcgatc ccgaaggaca
     3541 tggagccatg ggacgcaagg aactggactg cggcgcagaa agaggtggcc aatggcatcc
     3601 tgaaaaagaa gctggtggac gcgaaacgcc cggtgaggat gcgcttcaag gtgcatggcg
     3661 tggattactg ggggcgtttt gttttgtggt cgcatctgcc agccgacgaa ggctatgcca
     3721 tccgcatttt tgccagtcag tggagggagg cggacatgct gccaaagctg gccgctctgc
     3781 ggaagggcga tctcatcgaa atgacgggtt tgtgtgatct ggcaaagttc gaaaacctct
     3841 ggaacaccga ctcgctttcc ctgggcatag gaaatgccgc cttcatcaag ctccagcccg
     3901 acggctcgcc cgcgcccggg cctgagcggg tgccggttaa actggtttcc gcggtttatg
     3961 gatcgggcac ccagtttacg gatgtcacgg gacgtgtgaa ggcgctgctc gacgagccgg
     4021 gtgctgtctt tcatgccaac ccgcactggc tcggggcgga tccaacccct ggctggaaca
     4081 aagccctcgt tatcgtccat gagaatcggg gcgtccgcag cgtcttcacc accggtgaga
     4141 acggggaggt gagcgcggcc ttggttgtga aataagtggt ttcacgggtt atatccgccc
     4201 accttagcga cgccttcaga gcgttccggg gaatgggcaa tcttcccatc ttggcgggat
     4261 cataaaggaa cgccttattg acgctaggct ggcattgttg tcagactacg gaaggccgga
     4321 gcgctgatga aacgcgcacc ctccgccagg atgaaaaggc cgctgcaagc catgaaaacc
     4381 acgggacaac accgactgct gacaccgggc aatctgcttg tggtggcgct gtccgcagga
     4441 atgtgggccg ccgccttcat gctgccggtg cccgtgccgg cgcagccgct gcgcgtggcg
     4501 gtgcccctgt ggccgggagc ggagccgtgg gtgatggcgc gcgccgaggg gaaactgagg
     4561 ccggaccaaa tcaaactcgt tgagatcaac tggacctcgg cggcgatgcg cgcggtgggc
     4621 aaccgcgttg tggacgcggc gatcctttcc ctggaagagg cgctgctgca gatccaccaa
     4681 gggtacccac tgaagatcct gatggtgacg gatgtgtcac gcggggcgga tgcgttgctg
     4741 gtcaacccgg ggatagaatc tctggctgac ctgcgcggca ggcggattgg tttcgagccg
     4801 cgcacggcgg gaacccggct gctgggcctg gcgctcgccg aagcaggact gagcatggct
     4861 gacatccgcc atgtggtgat gaacgccacg gaactgcagg atgggggaat ggaccgtccg
     4921 gccgaggcgg tggtgtgctc ggagccgtgg cggcaaaggc tggtggcgcg cggcatgagg
     4981 ccgctttatg actctgggaa gaagggggcg gaggtggtga gagtgctggt ggcacatgtg
     5041 gacgcggtgg ctgaccacag ggacgagttg ctggtcctgg tgcgggcgca tgtggaggga
     5101 atgccgcgtc ttgccgcggc gggcgcggag ttggagccag tgctgcgccg ggagggggtg
     5161 tcggcggagg agttcgcccg gatcatggaa aagatggaag tgccggcatt ggagcagaac
     5221 cggcgctggc tggcgtgcga ggacggctgg ctggagtcgc gccttgagca gttgcgcgcg
     5281 gaactgctgc ctgcggacgg cctggagccg gcgcggcggg cggccgatgt ttttgacccc
     5341 tggtttttgg aggaggcacg atgaggcgcg cgctaacatc cctgcagttc aaggtttcgg
     5401 cctcgatttt cctgatgggc gcggtgctca tcacgctcag cgcggcgcgc atgacgagcc
     5461 gggccattga gtcccgccat gcgcgggtgg tggcgcatgc gtccatggag ggcggccgtc
     5521 tcgccggcat ggcgcagcat ttaatccgca gacaggcgcc gcatgtgatg gatctggagt
     5581 tgagttatgc ctccgcgcag caggatctgg agcttgggct gatcctggac gacacgggca
     5641 gggtgcggct ctccacccgg caggatctgc gcggactgcc cctggcggag acgcccatgg
     5701 cggaggcggc agaactggtg gatcaagcgc gcttttccat ggaaggcagg gtcgtggaag
     5761 ccgaggctgg aacaaaaatg cgcgcggtgt tccccttccg cgaagggctg aatgagcgcc
     5821 ggggctgcgt actgctggac ttcgacctgg agcgtccgct tgacgagggg atggcagcgg
     5881 ctctgcattc ctccatggtt caggggctgg cgctggccgg cggctgcctc gtgctgtggc
     5941 tgctgctaaa agtggtggtc accgaacgtg tctccatgct ggtgcggcaa gtggaggaca
     6001 tttccctgga gagtgatcct ggcgcgccgc tggagggcgc ggacgagatt tcccgtgtgt
     6061 cacaggccgc cagccggacg cacgcgcgtc tgcggcagtc agagcagagg ctgcgccagg
     6121 tggccgcgac gatgcgtgat gtgttctggc tggcgccggc ggaggccgcc caggagccat
     6181 atgtgaatcc ggcttatcag tccgtgctta agcgcgaccc tgcgcgcctg gccgctcggc
     6241 gctgggactg gcttcacgcg ctggccaggg aggaccgccg cccatgtctg gaacggctgc
     6301 ggcgtctgcg ctccggggag gtggtgccgg agtttgaggc gcgcgtggtt tcctccccag
     6361 gccagacgat ctggctgagg tgccgcggtt tttccgtgcc gggccacgcg ggcgccggac
     6421 gcatggtggg gggcatcgcc atggatgtga gcgagcgcaa aatgctcgac cgaaggctgc
     6481 tggacacggc ggaaaaagag cgccagcgcg tgggcgtgga tttgcatgac gacctctgcc
     6541 agaggctggc cgccgtgctg atgaagacag gcatcctgca ggccgcgctg gagcgggccg
     6601 agtctccgca gcaggcgctg gccgcggaac tggcgcggga tctgtcggag gcgaccgccc
     6661 tttcccgccg cttcgccaga gggctggcac cggtcagcat cgaggcgctc ggcctgccgg
     6721 cggcactcgc cgatctggga gatttcatca gccgggcttt caacatcccc tgccgcgtag
     6781 agtgcaccgg agccgagctg aactgcggcg cggagacagc cacgcacatc ttccgggtgg
     6841 cgcaggaact ggccaacaac gccgcgaaac acggcggatg ctcgtggatt gagatcgctt
     6901 ttgaagccgc cgcgcacgag gcccgcctgc gcatccggca tgacggacgc ccctacgacc
     6961 cccagcacag ccagggcgac ggcatgggca tgcacctcgt gcgccagcga ctcgacgcgc
     7021 tcggcgcctc cctcggagtc atctgtgacg aggagagcgg cgtggttcag gaatgccgta
     7081 tttctttgaa cgacaccccc aataccccga catcatgagc agcaccccat ccccaacccc
     7141 agccgcgcgc gcccaccggg tcgccgtcgt ggacgaccac accctcatgc gcgagggcat
     7201 gaaaatgctc ctcgaaaacc tccagggcct ctcctgcgcc tggaccgccg gagacacttc
     7261 cgaggcgctg cgattgctgg aggcagacat gcccgacgcc ctcgtggtgg acatcaccat
     7321 gcccggccgc aacggcctgg agctcatcaa ggacatccgc gccctgtatc ccgatctggc
     7381 cgtgctggtc gtctcgatgc atgacgaggc gctctacgcg cagcgcgccc tgaaggccgg
     7441 cgccaagggg tatgtgatga aggacgcgcc ccacggcgtc tttgaaaaag ccatccgtca
     7501 tgtgctcgcg ggcggcattg ccctgagcga gaagatgtcc gagagcatcc tgctggcgtt
     7561 ttcctccggg acgaatccag ggccggaggg cgccatccat aatctcagcg accgcgagtt
     7621 cgaggtcttc cagttgatcg gcgaggcgcg cagcaccgcc cagatcgccg ccgagctggg
     7681 catcagcccg aagacggtgg atgttcacaa gctgaaaatc cgccaaaagc tcagtctcac
     7741 agagagcacc tcgctgactg cctttgccat ccgctgggtt gagctgcgca agctcgacat
     7801 caaagagccg gcgca
//
LOCUS       DAOMYJ010000234         7813 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Prosthecobacter sp. isolate AMR_MDS_5629
            NODE_3096_length_7813_cov_20.090358, whole genome shotgun sequence.
ACCESSION   DAOMYJ010000234 DAOMYJ010000000
VERSION     DAOMYJ010000234.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867850
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prosthecobacter sp. (wastewater metagenome)
  ORGANISM  Prosthecobacter sp.
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Verrucomicrobiaceae; Prosthecobacter.
REFERENCE   1  (bases 1 to 7813)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7813)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.30
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:34:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,742
            CDSs (total)                      :: 4,684
            Genes (coding)                    :: 4,676
            CDSs (with protein)               :: 4,676
            Genes (RNA)                       :: 58
            tRNAs                             :: 55
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7813
                     /organism="Prosthecobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3096_length_7813_cov_20.090358"
                     /isolate="AMR_MDS_5629"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1965333"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..786
                     /locus_tag="PLP58_18490"
     CDS             <1..786
                     /locus_tag="PLP58_18490"
                     /inference="COORDINATES: protein motif:HMM:NF012488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HRJ10577.1"
                     /translation="ALRAALAPGRAALVSLLWANNETGVIAPMRELVETAHAAGAPVH
                     ADAVQAAGKMPLDLRAVPVDYLSLSGHKMHAPQGVGALYASRRAAFRPLLVGGGQERG
                     MRSGTENVPGIAAFGKAAELARARLHAAIPRMRDLRDLLEQRILAALPDAVIHGRDAD
                     RLPNTSSIRLPAADAAGMLILLDQKGIACSGGSACHSGALHPSHVLEAMGFDAAHAAG
                     TLRFSLSHLTTREEIETAAGLVIQAAEKIRALKDEASGEVIGV"
     gene            883..2034
                     /locus_tag="PLP58_18495"
     CDS             883..2034
                     /locus_tag="PLP58_18495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009962128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HRJ10578.1"
                     /translation="MLHNLFQLMSPETLPTEHTEETNARILAVSEDRVKGFMRQPFQF
                     IAEESGVPFDTVVERIRAMLKAGVIRRVRQTLLANKLAEGALVAWKLPVEKLEPAFEF
                     LFKEDPFSGHVVLRSTDREVSGSDFRLWTTLKVPQDRNINDHCDVLKRLIGAQDYYTM
                     QAHGIFALGVGHVRRKTMEPGEKADEPAKMMTTNIAELDAEEWNVLLHLKGDLSLEEI
                     GPEPWAGRAQAAGVSLEKFCEVAKRLDDKGVIGRFSTFLEHVKPSAAGVRVTRFNALF
                     HWRVPKGMEERAGGEVGRHPIMTHCYWREGGERFANVNIMGVIHGTDKETVLAHKAAI
                     DRHLEGIGIPVEYTNVFWGGRSEIKPSEISPVVFKAWHEKWAGVGGPVI"
     gene            complement(2090..2833)
                     /locus_tag="PLP58_18500"
     CDS             complement(2090..2833)
                     /locus_tag="PLP58_18500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ10579.1"
                     /translation="MNARLILACLIALPPLHAAEKKKPKDGEPPPPPAHLLFVNAVED
                     RGLAFVVLDGQDVQRRGFRNGQVTGWLGVASGARQVRVEHEPLGIVEKESDLAPGSHH
                     ALIAHNHSQPQPETGRPPRPAIGVLWVDCDEAARGRAGDDKVPLTVINATSQPEIMLK
                     TGALKLKARRLQPVKARVSAEGSFLSLTLEREEEKKDPPSDDSQEPQEASPLASVNIE
                     EKAAFFVVLIEKTPGEIAAATFSLALPAP"
     gene            complement(2838..3485)
                     /locus_tag="PLP58_18505"
     CDS             complement(2838..3485)
                     /locus_tag="PLP58_18505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ10580.1"
                     /translation="MRAIFKLALILAAAASAQEPEGGYARLRFVNATGMEGAVLVSLD
                     GQKLNSRGYTSGQATGHMDVLPKNYQIELKHDTLGESRLNLELRPGEMRAVIVLAKIG
                     ETKKEGEPPEITLDHHLLEYTVLEKGSPSSLLVLQTTPLPSLEISVAGKSCTAERFKP
                     ASMVVTGDMGQFPVVHFQQRRVCSLSFHEPADAALILYADAKGTLQHIFFKNHVR"
     gene            complement(3469..6591)
                     /locus_tag="PLP58_18510"
     CDS             complement(3469..6591)
                     /locus_tag="PLP58_18510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ10581.1"
                     /translation="MKTRFQGSDDRAFTLVMVLTILAAVTILVVGLFGIVNSERQISA
                     SFDAVEQADLAVQAGLARATAMLGQSLMDETGVVFAMPANVPLDEPRTPVPVLLTANY
                     DFTSKQWRYQPLISGAQAPAATATLQKPASTGLGYEPQPVNAQANQALDRQVMRRQHP
                     AALPSQAPPLAWWETLSLPASIPGPDGESAGQEKISARFCFHIEDLQGLLNLSHTGNL
                     DGTDERHSRHRQLINGPHVVPGLNLAFADRPLLNQSALYSIFNPLAQQDTSDLARHLV
                     KSRRLIFTPEAWKEIVIQPDTLTGWPGRDAAAYLARDAASSRFTETSARALEEHTISG
                     LESYFELALIPPDPAFVSDGRLQPGTRKLNLNRLLAAIEALPADQRQARAQQAVDEIA
                     GHIRTHLPDFDERRGGYPLGADPEFAYLQCLAAGIIDYADTDSMPTMKVLNETDPVTM
                     RGEYRGMDAFPIVSEQWQRYRFESSYTEDGSQFIDYSVTQYLELWNMTNKPVNGVIQS
                     TFECDGELTASAGIYDVKRILRGEEAPDATVKAGMPDQEGDFWWHPTIPVTLQPNQYI
                     VVRCQPVLLKLRSGLPAPPGATATVSLRGYQNNRDRTSKYRIRFGPAGSPLTLVDFPQ
                     GKLDRFDWAVNNTSNRQRFNTNLPGMSYSDANQAGTFANNIGDPRAAFFIDYLLDQVN
                     YPNNSSPWSRNIRFNNVTRFFGENRGSLWPDGGHNSPLRPSRVNDYNNNPDTPLIPAL
                     AVEPEKYVQRLSNEGRLFSVTELGHVFDPIMWDPDGGSPYEPPGDMQYRDFADIRPGD
                     MAKSSIFYCGGNTLRVGRPEHSRFRPAYGETIPNRPANRSMCATALLDLFHTGIPQAV
                     DEQVRAGDFQRIHGHVNINTASKQTLQALVAGALEMDTAIQYPAGSADAAIPRPGTPE
                     SASLIADEIILRRPFLSASELPERLLASDGQPLLGRTHRKSDREVFPEWNDSAAEEAF
                     ARLFNSSTVRSRHFRVVVTGQCYRESRSGAMNVLATRSRLYHVFVRPVRGEDGVILRN
                     DIQITYARNF"
     gene            complement(6588..7496)
                     /locus_tag="PLP58_18515"
     CDS             complement(6588..7496)
                     /locus_tag="PLP58_18515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HRJ10582.1"
                     /translation="MMPPRGRACMGAAAAPGMTLVELLVTLAVSSILLLALMQSLTTA
                     ADSWTNQSKNFSAQREARTCLRLLADDLAALVALPGTQFTSTPPPPGTSPLPRMRFLF
                     TASADDFGSARLAFLRTAKGRPRDGATARGDLQLVLYGTALTPDGGASSASRQFLSQK
                     LIRRVFTPEATWRRVRAHLEQNQPLVTDSDWQDLQNAAAAGSDAVNEPLAYDVIRFDA
                     KPLEEIHPALQTTTPWPEDRVPLWLDVTLRVTSRAIAAQLVTAEDWRGQGAFTRLLIN
                     DTPLDYEDDREARTYTLRLRLTDTEL"
     gene            complement(7510..>7813)
                     /locus_tag="PLP58_18520"
     CDS             complement(7510..>7813)
                     /locus_tag="PLP58_18520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ10583.1"
                     /translation="EGAVEGSEPPPEFALVDSALQPLLTTRQDSAQAQSEYQTGSQSP
                     RAMYFTKLRRLPLPDPADPLAAVPGITRLVITVESPASAPEGRRKVHRYVTLVPSS"
BASE COUNT         1263 a         2408 c         2754 g         1388 t
ORIGIN      
        1 gccctgcgcg ccgcgctcgc tccggggcgc gcggcgctcg tctccctgct ctgggccaac
       61 aacgagacag gcgtcatcgc gcccatgcgc gagctggtgg agacggccca tgccgccggc
      121 gcccccgtcc atgcggacgc cgtccaggcc gccggaaaga tgccgctcga cctgcgcgcc
      181 gtgcctgtgg attacctcag cctcagcggc cacaaaatgc acgcgccgca gggcgtcggc
      241 gcgttgtatg ccagccgccg cgccgctttt cgcccgttgc ttgtcggcgg cgggcaggag
      301 cgcggaatgc gcagcggcac ggagaatgtg ccaggcatcg ccgcctttgg caaagccgcc
      361 gaactcgccc gcgcgcggct gcacgccgcc atcccgcgca tgagggatct gcgtgatctg
      421 ctggagcagc gcatcctggc cgctctgccg gatgccgtca ttcatggccg cgacgcggac
      481 cgcctgccga acacgtcctc aatccgcctg cccgctgcgg atgcggcggg catgctcatt
      541 cttctggatc aaaaaggcat cgcctgctcc ggcggctccg cctgccacag cggcgcgctg
      601 catccctccc atgtgctgga ggcgatgggt ttcgacgccg cccatgccgc cggcacgctg
      661 cggttttccc tgagtcatct caccacccgg gaggagatcg aaaccgccgc cggcctcgtc
      721 atccaggccg ctgaaaaaat ccgcgcgctc aaagacgagg cctccggcga ggtcatcggc
      781 gtctgaggac ccgcgccccc gcttgccgta aaccatgccg tgcttgtcat tccgcgccgc
      841 gccgctaaga gtccaccccc gcgcggcgtc catctccgcg ccatgctgca caatctcttc
      901 caactcatgt ctccagaaac gctcccgacc gaacacaccg aggaaaccaa tgcccgcatc
      961 ctggcggtga gcgaggaccg tgtgaaaggc ttcatgcgcc agccctttca gtttatagcg
     1021 gaggaaagcg gcgtgccttt tgacaccgtg gtggagcgca tccgcgcaat gctcaaggcg
     1081 ggcgtcatcc gccgcgtgcg ccagaccctg ctggccaaca aactggccga gggcgccctc
     1141 gtggcctgga agctgccggt cgaaaagctg gagccggcat tcgagttcct tttcaaggag
     1201 gaccctttca gcggccatgt cgtcctgcgc tccaccgacc gcgaggtcag cggcagcgac
     1261 ttccgccttt ggaccaccct caaagtgccc caggaccgca acatcaacga ccactgcgat
     1321 gtgctcaaac gcctgatcgg cgcgcaggac tactacacca tgcaggcgca cggcatcttc
     1381 gccctcggtg tcggccatgt gcgccgcaag acgatggagc cgggcgagaa agcggacgag
     1441 ccagcgaaga tgatgaccac caacatcgcc gaactcgacg cggaggagtg gaatgtcctc
     1501 ctgcatctca agggcgacct ctccctggag gaaatcggcc ccgaaccctg ggccgggcgc
     1561 gcgcaagccg ccggagtcag cctggagaaa ttctgcgagg tggcgaaaag gctggatgac
     1621 aaaggcgtca tcggccgctt ctccaccttc ctcgagcatg tgaaacccag cgccgccgga
     1681 gtacgcgtca cgcgcttcaa cgcgctcttt cactggcgcg tgccgaaggg catggaggag
     1741 cgcgccggag gcgaggtggg ccgccacccc atcatgaccc actgctactg gcgcgagggc
     1801 ggcgagcgct tcgccaacgt caacatcatg ggcgtcatcc acggcaccga caaggagacc
     1861 gtgctcgccc acaaggccgc catagaccgc catctggagg ggatcggcat cccggtcgag
     1921 tacacgaacg tgttctgggg cgggcgcagc gagatcaagc ccagcgagat cagccccgtc
     1981 gtcttcaagg cctggcacga aaaatgggcc ggggtcgggg gaccggtgat ctgatccgca
     2041 gccgcgcctg caaaggcgtg gatgacccgc cctcgcgcgc gctgccgcct caaggcgccg
     2101 gcagggcgag gctgaaggtg gcggcggcga tctctccggg tgtcttctcg atcaggacga
     2161 cgaagaaggc ggccttttcc tcgatgttca cgctggccag cggggacgcc tcttgcggct
     2221 cctgcgagtc atcggagggc ggatcctttt tctcctcctc ccgctccagg gtcaggctga
     2281 gaaagctgcc ctcggcgctc acccgcgcct tcacaggctg gaggcggcgg gctttgagtt
     2341 tcagcgcgcc cgttttgagc atgatctccg gctggctggt ggcgttgatg acggtgagcg
     2401 ggactttgtc atcgcccgcc ctgccgcgcg cggcttcgtc acagtccacc cagagcacac
     2461 cgatggcagg gcgcggcggg cgtcccgtct caggctgcgg ctgggagtgg ttgtgcgcga
     2521 tgagggcgtg atggctgcct ggcgccaggt cggactcctt ttccacgatg cccagcggct
     2581 cgtgctccac ccgcacctgg cgcgcgcccg aagccacgcc gagccagcct gtgacctggc
     2641 cgttgcgaaa gccgcggcgc tgcacgtcct ggccgtcgag cacgacaaaa gccaggccgc
     2701 gatcctccac cgcgttgacg aaaagcagat gcgcgggcgg cgggggcggc tccccgtctt
     2761 tcggcttctt tttctccgcg gcgtggagcg ggggcagtgc gatcagacag gcgaggataa
     2821 ggcgcgcgtt catggggtta gcggacatgg tttttgaaaa agatgtgctg cagtgtcccc
     2881 ttggcgtccg cgtagaggat gagggcggcg tccgccggtt catggaagct gaggctgcac
     2941 acccggcgct gctggaagtg caccacgggg aactgcccca tgtccccggt gacgaccatg
     3001 ctggccggct tgaagcgctc ggcggtgcag ctttttcccg ccacgctgat ctccaggctg
     3061 ggcaggggag tcgtctgcag aaccaggagg ctggagggcg agcctttctc cagcacggtg
     3121 tattcgagca gatgatggtc cagcgtgatc tccggcggct cgccctcctt cttcgtttcg
     3181 ccgattttgg cgagcacgat gacggctctc atctcgcctg ggcgcagttc caggttgagc
     3241 cggctctccc ccagcgtgtc atgcttgagc tcgatctggt agttcttcgg cagcacgtcc
     3301 atgtgcccgg tggcctggcc gctggtgtag ccgcgcgagt tgagcttctg gccgtccagg
     3361 ctcacgagca ccgctccctc catgccggtg gcattcacga agcgcagccg cgcgtatccg
     3421 ccctccggct cctgggcgga ggcggcagcg gcgagaatga gggccagttt aaaaattgcg
     3481 cgcataggtg atctggatgt cgttgcggag gatgacgccg tcctcgccgc gcaccgggcg
     3541 cacgaagaca tggtaaaggc gcgagcgcgt ggccagcacg ttcatcgcgc cggagcgcga
     3601 ctcgcggtag cactgtccgg tgaccacgac gcggaagtgc cggctgcgca cagtgctgct
     3661 gttgaaaagg cgtgcgaagg cctcctcggc ggcggagtcg ttccattcgg ggaaaacctc
     3721 gcggtcggat ttgcggtggg tgcgtcccag caggggctgc ccgtctgatg cgagcagcct
     3781 ttccggcagt tcggaagcgg agagaaaggg gcggcgcagg atgatctcgt cggcgatcag
     3841 acttgccgac tccggcgtgc cggggcgcgg aatcgccgca tcggcgctgc cggcggggta
     3901 ctggatggcg gtgtccattt ccagcgcgcc ggccacgagc gcttgcagag tctgttttga
     3961 tgccgtgttg atgttcacat gcccgtggat gcgctggaag tccccggcgc gcacttgctc
     4021 atccacggct tgcgggatgc cggtgtggaa gaggtccagc agggcggtgg cgcacatgct
     4081 gcgatttgcg ggtctgttcg ggatcgtttc tccataagcc gggcggaaac ggctgtgctc
     4141 cggcctgcca acgcgcagcg tgttgccgcc gcagtagaaa atggatgatt ttgccatgtc
     4201 gccggggcgg atgtcggcga agtcccggta ctgcatgtcg cctggcggct cgtaagggct
     4261 gccgccgtcc gggtcccaca tgatggggtc aaacacatgc cccagctcgg tgacgctgaa
     4321 cagcctgcct tcgttggaga gacgctggac atatttttcc ggctcgacgg ccagggcggg
     4381 aatcagcgga gtgtcgggat tgttgttgta gtcgttgacg cgcgaggggc ggagcgggga
     4441 gttgtgcccg ccgtccggcc agaggctgcc ccggttttct ccgaaaaacc gtgtgacgtt
     4501 gttgaagcgg atgttgcgcg accaggggct ggagttgttc gggtagttca cctggtcgag
     4561 caggtaatct atgaagaagg ccgcgcgcgg atcacctatg ttgttggcga aagtgcccgc
     4621 ctggttggcg tcggagtacg acatccccgg caggttggtg ttgaagcgct ggcggttgct
     4681 ggtgttgttc accgcccagt caaagcggtc cagctttccc tgcgggaaat ccaccagagt
     4741 caaagggctg cctgccgggc cgaaacgaat acggtatttg ctggtacggt cgcggttgtt
     4801 ttgatagccc ctgagactga ctgtggctgt ggctcctggc ggcgcgggca aaccagaccg
     4861 aagtttcagg agcaccggct ggcagcgcac cacgatgtat tggttgggtt gcagcgtgac
     4921 cgggatcgtg ggatgccacc aaaaatcacc ctcttgatcg ggcattccgg ctttgaccgt
     4981 cgcatccggc gcctcctcgc ctctcaggat gcgcttcaca tcataaatgc ctgccgaggc
     5041 ggtcagttct ccgtcgcatt caaaggtgga ctggatgacg ccgttcaccg gcttgttggt
     5101 catgttccag agttcgagat attgagtgac ggagtagtct atgaactggc tgccgtcctc
     5161 tgtgtaggag gactcaaagc ggtacctctg ccactgctcg ctgacgattg ggaaagcgtc
     5221 catgccgcgg tattcgcccc gcatggtaac cgggtcggtc tcattgagga ccttcatcgt
     5281 cggcatggag tcggtgtcgg cgtagtctat gatgccggcg gcgaggcatt gcaggtaggc
     5341 aaactcaggg tccgcgccaa gagggtagcc tccgcgacgt tcgtcgaaat cgggcaagtg
     5401 cgtccggatg tgcccggcga tctcatccac cgcctgttgc gccctggcct ggcgttgatc
     5461 ggcgggcagg gcctcaatgg ccgcgagcag gcggttcagg ttcagcttgc gtgttcctgg
     5521 ctggagccgg ccgtcggaga caaaggcagg gtccggagga atgagcgcca gctcgaagta
     5581 ggattccaac ccgctgatgg tgtgctcctc cagggcgcgg gcgctggtct cggtgaagcg
     5641 cgatgaggcc gcgtctctcg ccagataggc cgccgcatcc ctgccgggcc agcctgtgag
     5701 tgtgtcgggc tggatgacga tctccttcca agcctcggga gtgaaaatga ggcggcggga
     5761 tttgacgagg tgcctggcga gatcggatgt gtcctgctgg gccagcggat tgaaaatgga
     5821 gtacagggcg ctttggttga gcaggggccg gtcggcgaag gccaggttga gtccgggcac
     5881 gacatgcggg ccgttgatca attgccggtg gcgtgagtgc ctttcgtccg tgccatccag
     5941 gtttccggtg tgggagagat tcagcagccc ctgcaagtcc tcgatgtgga agcagaaacg
     6001 cgcggagatt ttttcctgcc ccgcgctttc tccgtcaggg ccggggatgc tggcgggcag
     6061 ggaaagcgtc tcccaccagg ccagcggcgg tgcctgcgag ggcagggcgg cgggatgctg
     6121 gcggcgcatg acttggcggt ccagggcctg attggcctgc gcattgaccg gctgcggctc
     6181 gtagccaagg ccggtgctgg cgggcttttg cagcgtcgct gtcgcggcgg gcgcctgcgc
     6241 gccggagatg agcggctggt agcgccattg tttggaggtg aagtcgtagt tggccgtcag
     6301 cagcacgggc acgggcgtgc gcggctcgtc gagcggcaca ttcgcgggca tggcgaaaac
     6361 gacgccggtt tcatccatga gggactggcc gagcattgcg gtggcgcggg ccaggccggc
     6421 ctggacggcg aggtcggcct gctccacggc atcgaagctg gcggagatct ggcgctcgct
     6481 gttgacgatg ccaaagaggc caaccaccag gatggtcaca gcggcaagga tggtgagcac
     6541 catcacgagg gtgaaggcgc gatcgtcaga gccttggaag cgggttttca caattcggtg
     6601 tcagtcaggc gcagacgcag ggtgtaggtg cgcgcctcgc ggtcgtcctc gtagtcgaga
     6661 ggcgtgtcgt tgatcaggag gcgggtgaac gcgccttgtc cgcgccagtc ctcggcagtg
     6721 acgagctggg cggcaatggc gcggctggtg acgcgcaggg tcacatccag ccataaaggc
     6781 acgcggtcct ccggccaggg agtggtggtt tgcagtgcgg ggtggatttc ctccagcggt
     6841 ttggcgtcaa agcggatcac atcgtaggcc agcggctcgt tgacggcgtc ggagccggcc
     6901 gcggcggcgt tttgcaggtc ctgccagtcg ctgtccgtga ccagcggctg gttctgctcc
     6961 agatgcgcgc gcacgcgccg ccaggtggcc tccggggtga aaaccctgcg gatgagcttt
     7021 tgtgagagga actggcgcga cgcggaggac gcgccgccgt cgggtgtgag cgcggtgcca
     7081 tagagcacga gctggaggtc cccgcgcgcg gtcgcgccgt ctctcgggcg gcctttggcg
     7141 gtgcgcagga aggcgaggcg cgcggagccg aagtcgtcag cggaggctgt gaaaaggaag
     7201 cgcatccggg gcagtggact cgtgcccgga ggcggaggcg tggaggtgaa ctgcgtgccg
     7261 ggcagcgcga ccagcgcggc gaggtcgtcg gccagcaggc gcaggcaggt gcgcgcctcg
     7321 cgctgggcgg agaagttttt gctttgattc gtccaggagt cggcggctgt ggtgagactc
     7381 tgcatcagcg ccaaaagcag gatggaactg accgcgagcg tgaccagcag ctcgacgagt
     7441 gtcatgccgg gggcggctgc cgcgcccatg caggcgcggc cgcgaggcgg catcaagcgc
     7501 gcgccctcct caggatgacg ggacgagcgt gacatagcgg tggactttgc gccgcccttc
     7561 gggcgctgag gcgggggact cgacggtgat gaccagccgg gtgatgccgg gcaccgctgc
     7621 aagcggatcg gcggggtccg gcagcggcag ccggcgcagc ttggtgaaat acatggcgcg
     7681 cggcgactgt gatccggtct gatactccga ctgcgcctgg gcggagtcct ggcgcgtggt
     7741 gagcaggggc tgcaaggccg agtccaccag cgcgaactcc ggcggcggct cggaaccctc
     7801 gacggcgccc tcc
//
LOCUS       DAOMYJ010000235         7806 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Prosthecobacter sp. isolate AMR_MDS_5629
            NODE_3101_length_7806_cov_21.839892, whole genome shotgun sequence.
ACCESSION   DAOMYJ010000235 DAOMYJ010000000
VERSION     DAOMYJ010000235.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867850
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prosthecobacter sp. (wastewater metagenome)
  ORGANISM  Prosthecobacter sp.
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Verrucomicrobiaceae; Prosthecobacter.
REFERENCE   1  (bases 1 to 7806)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7806)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.30
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:34:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,742
            CDSs (total)                      :: 4,684
            Genes (coding)                    :: 4,676
            CDSs (with protein)               :: 4,676
            Genes (RNA)                       :: 58
            tRNAs                             :: 55
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7806
                     /organism="Prosthecobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3101_length_7806_cov_21.839892"
                     /isolate="AMR_MDS_5629"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1965333"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..624
                     /locus_tag="PLP58_18525"
     CDS             <1..624
                     /locus_tag="PLP58_18525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009964665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1501 domain-containing protein"
                     /protein_id="HRJ10584.1"
                     /translation="FETAFQMQTAAPEAFDLAQEPAHIRRLYGLDRKVKGPGADFAWQ
                     CLVARRLAERGVRFIELIDTGSRPNWDSHGEMKEHADLAHNVDQPTAALITDLKQRGM
                     LDETIILWATEFGRTPWREGKNGRGHHRDCFSVWVAGGGFKPGHVHGRTDDIGKYTEE
                     NPVEVHDLHATLLHQLGMDHERLTHRHAGRDFRLTDVHGRVMTELLA"
     gene            690..1397
                     /locus_tag="PLP58_18530"
     CDS             690..1397
                     /locus_tag="PLP58_18530"
                     /inference="COORDINATES: protein motif:HMM:NF020296.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
                     /protein_id="HRJ10585.1"
                     /translation="MTLTQIRTQLGAQACAKRAEAAKRFFKTGPGEYGEGDRFLGLTV
                     PQVRAVLPQTDALPEDDVLVLLRSEWHEERLLALLILVRRFAKARKNEAARARLVSLY
                     LKNTRWVNNWDLVDTSAPLILGEWLRTQERGVLDTLAGSNCLWQQRIAVLATLAFIRS
                     GEFGDTLRLCERFLAHRHDLMHKACGWMLREAGKRDAAVLRGFLDLHAPNMPRTMLRY
                     AVEKLPDAERRRYMSKS"
     gene            1427..1834
                     /locus_tag="PLP58_18535"
     CDS             1427..1834
                     /locus_tag="PLP58_18535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009965988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="very short patch repair endonuclease"
                     /protein_id="HRJ10586.1"
                     /translation="MADVFSKAKRSEVMSRIRSSGNRDTELALVALMRAHGITGWRRG
                     QKLPGLPDFVFRRERVCVFVDGCFWHGCPLHFRRPKSRRAFWDAKIARNKARDQRVAR
                     ELRASGWRVLRVWEHALKKPGPLLARLRRAIGA"
     gene            complement(1848..2693)
                     /locus_tag="PLP58_18540"
     CDS             complement(1848..2693)
                     /locus_tag="PLP58_18540"
                     /inference="COORDINATES: protein motif:HMM:NF012993.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein"
                     /protein_id="HRJ10587.1"
                     /translation="MKIAHCQFESWCGEFDHNLARFEEGMGRADAAGAVIASFPECFL
                     TGYPDTEELARAGAFAADSPEMTRVLDVTARHEALAIIGFNETRGVDLYNTALVAHRG
                     HLLGLYSKCAAYQKFHKQGRDFPVWEHKGLKFGVLICADGGYIEPARILALKGARVVF
                     APHFNYIRDSGLIQHFMKVRADHTARAIENGIYFVRGNNVVLDAAKSGITRNAGVGYG
                     DSYIMDPGGEILAQSRRHQEDFIMAEIDPDPRKDRAWGMSKSAWSWREFKPFMDEAMA
                     QADGG"
     gene            complement(2783..3598)
                     /locus_tag="PLP58_18545"
     CDS             complement(2783..3598)
                     /locus_tag="PLP58_18545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018290259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphatase"
                     /protein_id="HRJ10588.1"
                     /translation="MLTDYHTHTPLCHHAEGSPLDYARHARSIGLAEIGLSDHNPMPE
                     QFDNWRMALADLPRYFEMVDEARAALPDFPVRLGLECDYLEGREDWIGKTARMAEWDY
                     LIGSVHYIHDGIAVDDPKYLSRWSGAADIEAMWAAYWRLYEKMIRSGLFDFYAHPDLA
                     KRFGLRPAGDLRCYYEPVIQALADTRGVLEVSTAGLRKDVREIYPAREMLEMAFSAGV
                     PIVINSDAHTPGDVGADFDQALALARGAGYRKTVRFEKRRRVEADLPETWPPR"
     gene            complement(3651..4460)
                     /locus_tag="PLP58_18550"
     CDS             complement(3651..4460)
                     /locus_tag="PLP58_18550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ10589.1"
                     /translation="MKTLPLALALIVCAHTSRAQEKSGFASFIELPWRYVIGGHSAGK
                     WHDSEAAGRLLGKPGISYRVFSLKGPAGSVTAGKAAPEADVCPDVWMQKITPRPDPSA
                     PVTLGVNASWDPMPRAAVMADTGDGALAAVVRGMLVKIGIAGPEVKIHQRLQIDLDGD
                     GRAETLVAARRFVNEVELFSAAAGDYCLVFMERETAKGVTRQILHGDAYLRADPEAAP
                     VIHEITGLLDLDGDGRLEVITHTGYYEGGGCQVWQLRGDELVKVIEMDCGV"
     gene            complement(4481..4954)
                     /locus_tag="PLP58_18555"
     CDS             complement(4481..4954)
                     /locus_tag="PLP58_18555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ10590.1"
                     /translation="MLLKILLIILVIAIVLGTGMILEIRRERALREWASGIPGARLHW
                     PFIAAEHPSVPAAELVELLIQRAPVSWASAIETSGGSGDVWLVEYRATPPGKKSTRWF
                     TLVAWRRNDLGSCGPLEHADAGARTLGRWSCRVLGGLITVSMLHEILGEQNPRPR"
     gene            complement(4987..5229)
                     /locus_tag="PLP58_18560"
     CDS             complement(4987..5229)
                     /locus_tag="PLP58_18560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ10591.1"
                     /translation="MNEALMTPHEQVRGGTAQCRRLRLPLLLLAEQAGPDVLAGLSAL
                     MRKVPPHEKFPRISGFSQAGWTWRLAVRRGPPGCAG"
     gene            complement(5241..5975)
                     /locus_tag="PLP58_18565"
     CDS             complement(5241..5975)
                     /locus_tag="PLP58_18565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ10592.1"
                     /translation="MRYTNLDLLFTPEGAIVGAVTLALTILICRPRKKKADSGPPASK
                     DIVGRFRASMEINYQRWHDGVGYDLPLIAQATPEERARIEKLVLAEPVYDWRVVEALC
                     HLRTPDAIARLKNALATSADHSVRMAVTERAGDIVSPEERSAAIIAAVREAPLVKGLH
                     HALQQIPAFRRPEIIEEVRQAVLKREGDGVPHCAGLLLFLHGKAVSLFDNAHRDLLLR
                     FNTKDPAARAAACRDLFELIGDSPAA"
     gene            complement(6016..6651)
                     /locus_tag="PLP58_18570"
     CDS             complement(6016..6651)
                     /locus_tag="PLP58_18570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ10593.1"
                     /translation="MDGHTCLQTARPGLAAGRHPHGGDWAFKSGSSLRAILPGGGRDY
                     EDHTTLDERWKVRFTRDGEEVTRVTIQTEDDSSESFILFGGDGGETCPMPVWVVWGPD
                     GDKLALRLGDSPRFTRTLIFRLVDGAWKPVAMPEFHPKERLTLQGHGFQERDRLVDAE
                     HWQDTGTLVVKYFGSFAKGDEGDGWHQFVSVRIDAQGRAEVVGFLDVPGEE"
     gene            7225..>7806
                     /locus_tag="PLP58_18575"
     CDS             7225..>7806
                     /locus_tag="PLP58_18575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ10594.1"
                     /translation="MMRRTTLLGGIATLLATGSLRGFLKKERAALPRGGSIILPSSHK
                     AVLAGEFNHEADIPALIAYRKYRSTLAGSWECDGMIATGHAGQFASLEMFAQKVRGAL
                     TTSWSYADLKPGWFGGTRSSFPLEKLDGPHGPELQRELVVHVAGFWDVYDKPARMFAM
                     HHRARGLMIAVWILDSHGGLKKARQMAGDIAGSY"
BASE COUNT         1404 a         2567 c         2487 g         1348 t
ORIGIN      
        1 ttcgagaccg ccttccagat gcaaaccgcc gcgcccgagg ccttcgatct cgcccaggag
       61 cccgcccaca tccgccgcct ctacgggctc gaccgcaaag tgaaggggcc cggcgccgac
      121 ttcgcctggc agtgcctcgt cgcccgccgc ctcgccgagc gcggcgtgcg tttcatcgag
      181 ctgatagaca ccggctcccg cccgaactgg gactcccacg gcgagatgaa ggagcacgcc
      241 gacctcgccc acaacgtgga ccagcccacc gccgcgctca tcaccgatct caagcagcgc
      301 ggcatgttgg acgagaccat catcctctgg gccactgaat ttggccgcac cccctggcgc
      361 gagggcaaaa acggccgcgg ccaccatcgc gactgcttca gcgtctgggt cgccggcggc
      421 ggattcaagc ccgggcacgt gcatggccgc accgacgaca tcggcaaata caccgaggaa
      481 aaccccgtcg aagtccacga cctgcacgcc accctcctgc accagctcgg catggaccac
      541 gagcgcctca cccaccgcca cgccggccgc gacttccgcc tcaccgacgt ccacggccgc
      601 gtcatgaccg agctgctcgc ctgagcaaag tgggagacat tcatgttgcc cgctcgggtt
      661 tcgtgaaaga aatcgaggga tcatttccca tgacgctcac ccaaatccga acccagctcg
      721 gcgctcaggc ttgcgccaag cgggcggagg cggcgaagcg ttttttcaag acagggccgg
      781 gcgaatatgg ggagggggac aggtttctcg ggctcacggt gccgcaggtg cgggcggtgc
      841 tgccgcagac ggatgcgctg ccggaggatg atgtgctcgt gctgctgcgc tcggagtggc
      901 atgaggagcg gctgctggcg ctgttgatcc tggtgcggcg ctttgccaag gcgcgcaaga
      961 acgaggccgc gcgcgcgcga ctggtgagcc tgtatttgaa aaacacccgc tgggtcaaca
     1021 actgggacct cgtggacacg agcgcgccct tgatcctggg cgaatggctg cgcacacaag
     1081 agcgcggcgt gctcgacacc ctggcgggat cgaattgcct gtggcaacaa cgcatcgctg
     1141 tgctggccac gctggccttc atccgcagtg gagagttcgg cgacacgctg cggctgtgcg
     1201 agcggttcct cgcgcacagg cacgacctga tgcacaaggc ctgcggctgg atgctgcgcg
     1261 aggcgggcaa gcgcgatgcg gcggtgctgc gcgggtttct agatcttcat gcgccgaaca
     1321 tgcccaggac gatgctgcgt tatgcggtcg aaaagctgcc tgacgcggag cggcggaggt
     1381 atatgtcgaa aagctgagcg gaggcgcgca ggatcccatc attcccatgg ccgacgtgtt
     1441 ttccaaagcc aagcgcagcg aggtgatgtc gcgcatccgg tcgagcggga accgggacac
     1501 ggagctggcg ctggtggcgc tgatgcgggc gcatgggatc acgggctggc ggcgcgggca
     1561 gaagctgccg gggctgccgg attttgtgtt tcgcagggag cgggtgtgcg tgtttgtgga
     1621 cggctgcttc tggcatggct gcccgctgca tttccgcagg ccgaaatccc gccgcgcgtt
     1681 ttgggacgcg aagatcgcgc gcaacaaggc gcgggaccag cgggtggcgc gggagctgcg
     1741 cgcctcgggc tggcgggtgc tgcgagtctg ggagcacgcg ctgaagaagc cggggccgct
     1801 gctggcgcgc ctgcgcaggg cgattggcgc gtgagcggtg cctggcatca cccgccatcc
     1861 gcctgcgcca tggcctcatc catgaatggc ttgaactcgc gccagctcca ggccgacttg
     1921 ctcatgcccc aagcgcggtc tttgcgcgga tcgggatcaa tctcggccat gatgaagtcc
     1981 tcctgatgcc gccggctttg ggcgaggatc tcgccgccag ggtccatgat gtaactgtcg
     2041 ccgtagccca cgcccgcgtt gcgcgtgatt ccgcttttcg ccgcgtccag caccacgttg
     2101 ttgccgcgca caaaatagat tccgttctcg atggcgcgag ccgtgtggtc cgcgcgcacc
     2161 ttcatgaagt gctggatcag cccgctgtcc ctgatgtagt tgaaatgcgg ggcgaagacc
     2221 acccgcgcgc ccttcagagc caggatgcgc gccggctcga tgtagcctcc gtccgcgcag
     2281 atgagcacgc cgaacttcaa gcccttgtgc tcccaaaccg gaaagtcgcg tccttgcttg
     2341 tggaacttct gatatgccgc gcacttgctg tacaacccca gcaagtgccc gcgatgcgcc
     2401 accagcgccg tgttgtacag gtccaccccg cgcgtttcat tgaagccgat gatggccagc
     2461 gcctcatgcc gcgccgtcac atccagcacc cgcgtcattt cgggtgaatc cgccgcgaag
     2521 gcgcccgcgc gcgccagctc ctccgtgtcg ggataaccgg tgagaaaaca ctccggaaag
     2581 ctcgcaatca ccgcccctgc cgcgtcggcc cgccccattc cctcctcgaa gcgcgcgagg
     2641 ttgtggtcaa actccccgca ccatgactca aactggcaat gcgcgatttt cattcgtgca
     2701 tcttccctca tcacacccgc aggagtcaat gaccgagtcg gccgcccgcc ggagttcaca
     2761 cggggcgtgt tccggcgctc cttcagcgcg gcggccaggt ctcgggcagg tccgcctcga
     2821 cgcgccgcct tttttcgaag cgcacggttt tgcggtagcc ggctccgcgc gccagggcca
     2881 gagcctggtc gaagtccgcg ccgacatcgc ccggcgtgtg ggcgtccgag ttgatgacga
     2941 tgggaacgcc cgcgctgaaa gccatctcca gcatctcgcg ggcgggataa atttcgcgaa
     3001 catccttgcg cagaccggcg gtgctgacct ccagcacgcc gcgcgtgtcc gccagcgcct
     3061 gaatgaccgg ctcataataa cagcgcaaat ccccggccgg acgcaggccg aagcgcttgg
     3121 ccaggtcggg atgcgcgtaa aaatcaaaga gaccgctgcg gatcattttt tcataaagcc
     3181 gccagtaggc tgcccacatg gcctcgatgt ccgccgcgcc gctccagcgt gagaggtatt
     3241 tcggatcatc cacggcgatg ccgtcatgaa tgtaatgcac gctgccaatg aggtagtccc
     3301 actccgccat gcgcgcggtt ttcccgatcc agtcctcgcg accctccagg taatcgcact
     3361 ccaggccgag ccggacgggg aaatccggca gcgccgcgcg cgcctcgtcc accatctcga
     3421 aatagcgggg caagtcggcc agggccatgc gccagttgtc gaactgctcc ggcatcggat
     3481 tgtggtccga gagaccgatc tccgccagac cgatggagcg cgcgtgacgg gcgtagtcca
     3541 gagggctgcc ctcggcgtga tgacaaagcg gagtatgggt gtggtaatcg gtgagcacag
     3601 agtctttctg gcccggcttg gcagaccgcg caagccggga gaggcggtgc tcacacgccg
     3661 cagtccatct cgatgacttt cacaagctcg tcaccgcgca actgccagac ctggcagccg
     3721 cctccctcgt agtatcccgt gtgagtaatg acctccagcc gcccgtcacc gtccagatca
     3781 agcaatcctg tgatctcgtg aataacgggc gcggcttcag gatcggctcg cagataggca
     3841 tcgccgtgga gaatctgccg cgtcacacct ttcgcggtct cgcgctccat gaagaccagg
     3901 cagtaatccc cggccgcggc gctgaagagt tccacctcgt tcacaaaccg cctggccgcc
     3961 accagtgtct cggcgcgccc gtcgccgtcg agatcaatct gaagccgctg atggattttc
     4021 acctccgggc ctgcgatgcc gatcttgacg agcatgccgc gcaccaccgc tgccagagcc
     4081 ccgtccccgg tgtccgccat cacagccgcg cgcggcatgg gatcccatga ggcgttcaca
     4141 ccgagagtga caggcgccga aggatccggc cggggcgtga ttttttgcat ccacacatcc
     4201 gggcagacat ccgcctcagg cgcggccttg ccggcggtga cactgccagc cggacccttg
     4261 agggaaaaaa cacggtatga aattccgggc ttgcccagca gccggccggc agcctcgctg
     4321 tcgtgccatt tcccggcgga atgaccgccg atgacataac gccagggcag ctcgatgaag
     4381 ctggcgaagc cggatttctc ctgggcgcgc gaagtgtgcg cgcagacaat gagggcgagc
     4441 gcgagcggga gcgttttcat gagggcaatg acagttggtt tcagcgcggg cgggggtttt
     4501 gttcgcccag gatttcatgg agcatggaga cagtgatcag gccgccgagc acacggcagg
     4561 accagcggcc cagagtcctt gcgccggcgt cagcatgctc caaaggcccg caacttccaa
     4621 ggtcattcct ccgccaggcg acgagtgtga accagcgtgt ggacttcttc cccggaggag
     4681 tggcacggta ttcgacgagc cagacatccc cgctgccgcc actggtctca atggccgacg
     4741 cccacgacac cggggcgcgc tggatgagga gttccacaag ctccgccgcc ggcacggagg
     4801 gatgctcagc cgcaatgaag ggccagtgca gccgcgcgcc cggaattccc gatgcccact
     4861 cgcgcaaagc gcgctcgcgg cggatttcca ggatcatgcc ggtgccgagg acaatggcga
     4921 tgacgaggat gatcagcaga attttgagaa gcatgagggt tcgcatggtt gggggctttc
     4981 actccgtcag ccagcacagc caggcggccc ccgccgcacg gcgaggcgcc aggtccatcc
     5041 agcctgggag aagccggaga tccgcgggaa tttctcatgc ggcggcactt tgcgcatgag
     5101 cgcggaaagc ccggcgagga catccgggcc ggcctgctcg gcgagcaaaa gcagcggcag
     5161 tctgagccgc cggcactggg cggtcccgcc ccgcacctgc tcatgcggag tcatgagcgc
     5221 ttcgttcatc tgccgtgtct tcatgccgcg ggtgagtccc cgatcagttc aaacagatcg
     5281 cggcatgccg cagcgcgcgc ggccggatcc ttggtgttga agcggagcag gagatcacga
     5341 tgcgcattgt caaaaaggct gacggctttg ccatgcagaa acaacagcag tccggcgcag
     5401 tgagggactc cgtccccctc gcgcttcaac acggcctgcc gcacttcctc gatgatctcg
     5461 ggccggcgga atgcaggtat ctgctgcagc gcgtgatgaa gccctttgac caggggcgcc
     5521 tcgcgcactg ccgcgatgat ggccgccgaa cgctcctccg ggctgacgat atcaccggca
     5581 cgctccgtga ctgccatcct caccgagtga tcggcacttg tggcaagcgc gttttttaac
     5641 ctggcgatgg cgtccggcgt gcgcaaatga cagagcgcct cgacaacacg ccagtcataa
     5701 acgggctcgg cgagaaccag cttctcaatc cgcgcgcgct cttcgggagt ggcttgggcg
     5761 atgagcggca aatcataccc aacgccgtcg tgccagcgct ggtaatttat ttccatgctg
     5821 gcgcgaaaac gcccgacgat atccttcgaa gccggcggac cggagtccgc ttttttcttc
     5881 cgcggccggc agatgagaat cgtcagggcc agtgtcaccg cgccgacaat ggcgccttcg
     5941 ggtgtgaaca gcagatcgag gtttgtgtaa cgcatgatgt ttaatatcgg gtgttgtgtg
     6001 ttgtgattga aagcttcatt cctctccagg cacatccagg aatccgacga cttccgcccg
     6061 gccctgagcg tcaatcctga cgctgacaaa ctgatgccag ccgtcccctt cgtccccctt
     6121 cgcaaagctg ccgaagtact tcacgaccag cgtgccggtg tcctgccaat gttccgcgtc
     6181 cacgagccgg tcgcgctctt gaaaaccgtg tccttgcaaa gtgagccttt ccttggggtg
     6241 aaattccggc atcgccacgg gcttccacgc gccgtccacc agccggaata tcagcgtgcg
     6301 tgtgaagcgc ggactgtcgc ccaggcgcag cgcgagcttg tcaccatccg gcccccagac
     6361 gacccacacc ggcatcgggc atgtctcgcc gccatcgccg ccgaacaaaa tgaacgactc
     6421 ggaggagtcg tcctccgtct ggatggtgac acgggtgact tcctctccgt cgcgggtgaa
     6481 gcgcaccttc cagcgctcgt cgagcgtggt gtggtcctcg taatcccgcc ccccgccggg
     6541 caggatggcg cgcagtgacg agccggactt gaaggcccag tcacccccat gcggatgtcg
     6601 tccagcagcc agccctggcc gcgccgtttg aagacaagtg tgtccgtcca cgagaccggc
     6661 ggcaggcctt gcgagacgtg ggtttggcgc acttgcaccg tcgcgtccac agtcggggcg
     6721 ctgaacaccc cgccgatctc ccgggaggtc acaccctcaa acatgctgct gaacaaatcg
     6781 ccctcgatcc agtaaggctt ctcatcggga tgcctgcgca tttgctcccg ctggaggacg
     6841 cgggcgcgct ggaagatcga gaccagctcc ggcgtcagca gcggggacag ccgctgcaac
     6901 tgcgcctccg agggcaggcc gctgattcgt tcgcgatgca gcccggcgca gaatgccgag
     6961 gcagcttcgc ggatggcggc gcgttccgcg gcaagggtgg atttttcttc ggcggcggtg
     7021 agatgaccag ccaggctcga agaaatggcg agaatgaaaa gatggcgcgc gggtttcatg
     7081 atgtcggatg gaacagtgac agggaagctg cggatcaacc tccctggctg gcgcatcatc
     7141 actcatccgg cgcggaccgg gtatccgact ttagtcggat gcgcgcgggc ttgccgtcag
     7201 gacgggatca ggcagattca gaccatgatg aggcgcacca cacttctcgg cggcatcgcc
     7261 actctcctcg ccaccggctc cctcaggggc ttcttgaaaa aggagcgcgc cgcgcttccc
     7321 cgcggcggct cgatcatcct gccctcctcc cacaaggcgg tgctcgcggg cgagttcaat
     7381 catgaggcgg acatcccggc attgatcgcc taccggaaat accgctccac cctggcggga
     7441 tcgtgggagt gcgacggcat gatcgccacc ggccatgcag ggcagttcgc gagcctggaa
     7501 atgttcgcgc agaaggtgcg cggcgcgctt accacaagct ggtcctacgc ggacttgaaa
     7561 ccaggctggt tcggcggcac gcgcagcagc ttcccgctgg agaagctgga cgggcctcac
     7621 ggccccgagc ttcagcgcga gctggtggtg catgtcgccg gtttctggga tgtttatgac
     7681 aagcccgcgc gcatgttcgc catgcaccac cgggcgcgcg gcctcatgat cgcggtgtgg
     7741 attctcgact cgcatggagg cttgaaaaag gcgcgccaga tggccggcga catcgccgga
     7801 tcctat
//
LOCUS       DAOMYJ010000236         7718 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Prosthecobacter sp. isolate AMR_MDS_5629
            NODE_3150_length_7718_cov_22.930706, whole genome shotgun sequence.
ACCESSION   DAOMYJ010000236 DAOMYJ010000000
VERSION     DAOMYJ010000236.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867850
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prosthecobacter sp. (wastewater metagenome)
  ORGANISM  Prosthecobacter sp.
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Verrucomicrobiaceae; Prosthecobacter.
REFERENCE   1  (bases 1 to 7718)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7718)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.30
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:34:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,742
            CDSs (total)                      :: 4,684
            Genes (coding)                    :: 4,676
            CDSs (with protein)               :: 4,676
            Genes (RNA)                       :: 58
            tRNAs                             :: 55
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7718
                     /organism="Prosthecobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3150_length_7718_cov_22.930706"
                     /isolate="AMR_MDS_5629"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1965333"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            167..586
                     /locus_tag="PLP58_18580"
     CDS             167..586
                     /locus_tag="PLP58_18580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ10595.1"
                     /translation="MAPRPPRPGLVPTCAEGGVLGVLTGLVGTLQALEVIKLVTGIGT
                     PFIGKLLHMDTLGVRFRTFNLRRDPACPFCGENPSITEPIDYTGFCGMTPPPDVPTLT
                     VHDLHSLRQQGQPHFLLDVREPDEHATARIAGSTLIR"
     gene            621..770
                     /locus_tag="PLP58_18585"
     CDS             621..770
                     /locus_tag="PLP58_18585"
                     /inference="COORDINATES: protein motif:HMM:NF012790.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HRJ10596.1"
                     /translation="MQVLVHCNLGGRSARAVAALRDLGYDNVWNIAGGITAWSRKIDT
                     TVPQY"
     gene            985..6045
                     /locus_tag="PLP58_18590"
     CDS             985..6045
                     /locus_tag="PLP58_18590"
                     /inference="COORDINATES: protein motif:HMM:NF012271.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin type III domain-containing protein"
                     /protein_id="HRJ10597.1"
                     /translation="MKLPSFSSLCLGFTGFALAAAMQAETVRFENAGDLASYSVLASD
                     GTVTLTHAADLGAGSPASGGLVHHSTTASDKSTVLLKKVTATAPLASLWEQSVLISPR
                     EVDDALSGTEKKIETRLGFTATTTSGGNLKEFLHKTNPSFGVLVKAEHKPSDANKLRK
                     LEVFGFHNAAGSETKFGTLAFNESDFVDHWLRATITARRVAGDVFDLSFKVESLGVDG
                     TSAPVPVHSGSAAGVANAALLAASNLYTAVVTNTDKSTDSRSYLDDHTVRLVMPFEQA
                     GDFASYTTATTDGTVTLVEEAGAGAGNPATTGLTFISTTTSDRTAVVRKTVTGSGGAV
                     TLWRQSMLFNVREVDDSGVGEPKVETRMGFSVPMTLTGNYKELLHKVAANKNIHVKLK
                     AEHKASDVNKHRNIEVELTNYITSESKSGKVTLNNTGYFDDWLRLTLTLVRASATTFD
                     VTYTLEALGLDGTAEPFLLAQHTQNGVTNADFGGAASAEAVFLVNSDKAANNAWVDDH
                     SLILDAVPPAAPVALEAVNVTTNALTANWQAAAGAGGYVLELTTQAANFGAGTFLAAD
                     GTPGQAQGISIAGGGTLSQAVTGLSPSTPYVYRVKAVNGAGESAPSNVILAATLSDGA
                     NVPPTLDPIADYPLLTPGAPEQSIALTGISAGFGESQPLVVTVTSSHPAIIPHPVLLY
                     TSPQSTGTLLFKPAGAEGTSTITVTVDDGQPEDNLITRTFDISVRQPPLFVDFNEAED
                     MDEYAITNLRGSLVQAAGAGAGDPATGGAVFQGQGTGSDHGTLALRSQPYPGPAPTLM
                     RQTVWVNFREINDNLEIKDKGEIRFGFASHNVVPGELKKYFEEGSGKYAVHCKVMAEN
                     DPGDTSKDHVIKARVTNWNGSTKTEGPELVVSDESIFDHWFKCTFEALAVGTVQFQLR
                     FKVEDYGPDGTQLLGTVLESEPVNFLNSGLIDAPWIYAGLYADSEKSTTSRVFIDDHL
                     VEVMNVPPETPVAIEAYQITAHSFTARWDVPAGPYPNRFVVEAVPLAADFTAGNFINA
                     LGQGGQAAGVMVNFTEQRALRFFNLPSSTSYKYRVRSLNVAGASGNSNAVSVTTLLEG
                     SNAAPTLDVIADLGDIAADSGEITVPLTGVSDGGEFNQFVDITASSDNPALLPLLEVD
                     YFDPEDSGLLRFTPAPGQTGTAIITVTADDGQAVNAITTRSFVVRVVTPVSATAFDSA
                     GDAADYLIFAEQASLVFAGGEGAGTPPGGAMKFERSALPAEHVALALRRLRFDATVVP
                     HASTSLMFNLRNVPEIATGKDKAEISLGFISEPAPGAKMKDTFNKTHPSLFVKFKLEH
                     DTADLSKDRKLEVETASFDGFNEIKNSKNTLNAFLQAANWLRLSFKFIRTGFDTYLCS
                     YEVHDCGPDGTSEPALLMSGAPASFTNMPFFNDSSIYAGFVVNGEKQGNTPFWLDNHV
                     IDVNTTAADAPVNQAANDFVRTGFTLNWVGGILGREPTGYRVELCKAEDGFAPGTLIH
                     ASGAGGHDQGFAIADPWATDFLIRGLQPGQSYRYRVRAMRGAEASEVQDSILATLPSE
                     PAAPTFEEWRELHFAGDLENPLTLAHADFGGHGFPNLLLYALGLDPRTPPPSALLPRV
                     LPQDGYLTLTYQRRANLPEIQVIPVGCGDPSSWSSAGLTTLSVSEPDENGLETVVVRD
                     AFPIASKNRRFMRLHLAE"
     gene            6048..6608
                     /locus_tag="PLP58_18595"
     CDS             6048..6608
                     /locus_tag="PLP58_18595"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HRJ10598.1"
                     /translation="MFADAHPAIHAWLTQRDQSAAAWLVDTHRPHILRAARAWGAPPE
                     MEEDAVQEVLLRVFRALPRYEPREPFAHWLGVITRNTCAKLRRRWCHRHKLSACFENA
                     AADVADIELPHTRAPDRQMMLAERLDQLGAALGQLSPREREILERQTLDHEPAETTAA
                     SLDMKAGAVRTSLCRLRKQLRAALEE"
     gene            6739..7329
                     /locus_tag="PLP58_18600"
     CDS             6739..7329
                     /locus_tag="PLP58_18600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ10599.1"
                     /translation="MKPPQKPASKSPAPQPTWWSPKAALGLAVGFAAVAGFPYLASTT
                     RLLAEGKPVTIAAVKDETKKDMMKKEKEHHEHDAKKQREIKSMTVAADGTLYAGGHAG
                     VLRWKDGAWEKLPGFEDEEAKSIVSAPDGSLLVAGKHAAWKHKDGAWSKVYDGDTHSI
                     SQAADGTLYVAGPKVGLKKKAPGGEWEMVSEGFEGK"
     gene            7333..>7718
                     /locus_tag="PLP58_18605"
     CDS             7333..>7718
                     /locus_tag="PLP58_18605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ10600.1"
                     /translation="MASNWKLTNRVWHANLGMLSAITLGLVALSCPVIAHKWEAGKFL
                     EDIHYGKFLPPQTRWIWIDGQGFLLAFLIVSGLLMHRKSVKKAASTAADDPAVAGSSV
                     TVFGLGADAPVIALTEAAEKAGLRAFR"
BASE COUNT         1462 a         2634 c         2330 g         1292 t
ORIGIN      
        1 gcacatggcc cgcgtgcagc agatcgaagc agccgttggt gaacaccagc tttttgcccg
       61 ccgcatccag cctgtcgcgc aggcggcgga catcttcggc ggtcatgggc tggaactcgg
      121 ggctggacat gcggttttat gaagcctttt caccttcccg gcaagcatgg ccccgcgccc
      181 gccgcggcct ggtctcgtgc ccacctgcgc ggagggcggc gtgttgggcg tgctgaccgg
      241 cctggtcggc acactccagg ccctggaggt catcaagctt gtcaccggca tcggcacacc
      301 gttcatcggg aagctgctgc acatggacac cctcggcgtg cgcttccgca ccttcaacct
      361 gcggcgcgac cctgcatgcc ccttctgcgg cgaaaatccc tccatcaccg aacccataga
      421 ctataccggc ttctgcggca tgacacctcc acccgacgtc cccaccctca ccgtccacga
      481 tcttcacagc ctccgccagc aggggcagcc ccacttcctg ctcgacgtgc gcgagcccga
      541 cgagcacgcc accgcccgca tcgccggttc caccctcatc cggtgaggtc acagcccgcg
      601 ctggagaaat cccgcgcgat gtgcaagtgc ttgttcattg caatctcggc ggccgcagcg
      661 cccgcgccgt cgccgccctg cgcgacctgg gctacgacaa cgtctggaac atcgccggcg
      721 gcatcaccgc ctggtccagg aagatagaca ccaccgtgcc gcagtattga gccggtaaaa
      781 atccccgctg ggcaaagggt ggcggcgttt tgcaaaaacg cagcgcggtg cttggcaaag
      841 ttgcaacccc gcattcaggc acactggcgt gcgacatggt gagactttgc attttgtcgt
      901 ttgactaatg ggatgatgtc gcaagaatag cgctcctgag attcagtcgc acttcaagtg
      961 ggcgcggcca atctatgcat gcgcatgaaa cttccctcat tttcctccct gtgccttggg
     1021 ttcactggct ttgctttggc tgctgcgatg caggcggaga cagttcgttt tgagaatgcc
     1081 ggcgaccttg ccagctactc ggtactggcc tcggacggca cagtcacgct gactcacgcg
     1141 gcggacctgg gcgcgggcag cccggcgtca ggagggctgg tgcatcactc caccaccgcc
     1201 tcggacaaga gcacggtgct gctgaaaaaa gtcaccgcca ccgcgccgct cgccagcttg
     1261 tgggagcagt cggtgctcat ctcgccgcgc gaggtggacg acgcgctgag cggaacggag
     1321 aagaagatcg agacgcgctt gggattcacc gcgacgacaa catcgggcgg caatttgaag
     1381 gagtttctgc acaagacgaa cccctccttc ggcgtgcttg tgaaagccga gcacaagcct
     1441 tcggacgcga acaaacttcg caagctggaa gtctttggtt tccacaacgc ggcgggatcg
     1501 gagaccaagt tcggcacgct ggccttcaat gaatcggact tcgtggacca ctggctgcgc
     1561 gccaccatca cggcgcggcg cgtggccggg gatgttttcg atttgagctt caaggtggag
     1621 tccctgggcg tggacggcac ctcggcaccg gtgcctgttc acagcggctc tgccgccggc
     1681 gtggcaaacg cggccttgct cgccgcctcc aacctttaca cggcggtcgt caccaacacg
     1741 gacaagagca cggacagccg ttcctatctc gacgaccaca cggtgcgcct ggtgatgcct
     1801 tttgagcagg cgggcgattt tgcctcctac accacagcca cgacagacgg tacggtgacg
     1861 ctggtggagg aagccggggc tggcgcggga aatcccgcca cgacggggct gacgttcatt
     1921 tccacaacca cttccgaccg caccgccgtc gtgcgtaaaa cagtgacggg cagcggcgga
     1981 gcggtcacgc tttggcggca gtccatgctt tttaatgtgc gcgaggtgga tgactccggc
     2041 gtgggtgaac cgaaggtgga gacacgcatg ggcttcagcg tgcccatgac gctgacgggc
     2101 aactacaagg aactgctgca caaagtggcg gcgaacaaga acatccacgt caagctcaag
     2161 gccgagcaca aggccagcga cgtgaacaag caccgcaaca ttgaggtgga gctgaccaac
     2221 tacatcacgt cggagtccaa gtccggcaag gtcacgctga acaacacagg ctacttcgac
     2281 gactggctgc gcctcaccct gacgctggtg cgcgcctcgg ccaccacctt cgatgtgact
     2341 tacaccctgg aggcgctcgg tcttgacggc acggcggagc ctttcctgct ggcccagcac
     2401 acgcagaacg gtgtcacgaa cgccgacttc ggcggcgccg ccagcgccga ggcggtcttt
     2461 ctcgtcaaca gcgacaaagc cgccaacaac gcctgggtgg atgaccacag tctcattctc
     2521 gacgccgtgc cgcccgccgc gccggtggcg ctggaggccg tgaatgtcac caccaacgcg
     2581 ctcaccgcga actggcaggc ggcggcgggc gcaggggggt atgtgctgga gctgaccacg
     2641 caggccgcaa acttcggggc gggcaccttt ttggccgcgg acggcacgcc gggccaggct
     2701 cagggcatca gcatcgcagg cggcggcact ctcagccagg ctgttaccgg actctcgcca
     2761 agcactccgt atgtctatcg ggtcaaggcg gtgaacggcg cgggtgaaag cgcgccatca
     2821 aacgtcatcc tggccgcgac tttgagcgat ggagcgaatg tgccgccgac gctggacccc
     2881 atcgcggact atccgttgct cacgccgggc gcgccggagc aaagcatcgc gctcacgggc
     2941 atcagcgcgg gttttggtga aagccagccg ctggtcgtca cggtcacctc ctcgcatccg
     3001 gccatcatcc cgcatcccgt gctgctttac accagcccgc agagcacagg cacgctgctt
     3061 ttcaaaccgg cgggcgccga gggcacgtcc actatcacgg tgacggtgga cgacggccag
     3121 ccggaggaca atctcatcac gcgcaccttt gacatcagcg tgcgccagcc gccgctgttc
     3181 gtggacttca acgaggcgga ggacatggac gaatacgcca tcaccaacct gcgcggcagc
     3241 ctggtccagg cggcgggagc gggcgcgggt gatccagcca ctggcggcgc ggtgtttcag
     3301 ggacaaggca ccggctccga ccacggcacg ctggccctgc gctcgcagcc ttatcccggc
     3361 ccggctccca cgctgatgcg ccagaccgtc tgggtgaact tccgcgagat caacgacaac
     3421 ctggagatca aagacaaggg cgagatccgc ttcggctttg ccagccacaa cgtcgtgccc
     3481 ggcgagctga aaaaatattt cgaggaaggc tccggcaaat acgcggtgca ttgcaaagtg
     3541 atggcggaga acgatccagg cgacacgagc aaggaccacg tcatcaaggc gcgcgtgacg
     3601 aactggaacg gcagcacaaa gaccgaggga cccgagctgg tggtcagcga cgagagcatc
     3661 ttcgaccact ggttcaaatg caccttcgag gcgctggccg tgggcaccgt gcagttccag
     3721 ctccgcttca aagtggagga ctacgggccg gacggcacgc aactgctggg cacagtgctg
     3781 gagtctgagc cggtgaactt tctcaacagc ggcctgattg acgcgccgtg gatttacgcc
     3841 gggctatacg cggactcgga gaagagcaca acgtcgcgcg tgttcattga cgaccatctc
     3901 gtggaggtca tgaacgtgcc gccggaaacg ccggtggcga ttgaggcgta tcaaatcacc
     3961 gcgcactcct tcaccgcgcg ctgggatgtg ccggccgggc cgtatccgaa ccgcttcgtc
     4021 gtcgaggccg tgccgctggc tgcggacttc acggcgggca atttcatcaa cgccctcggc
     4081 cagggcgggc aggcggcggg cgtgatggtg aacttcaccg agcagcgcgc gctgcggttc
     4141 ttcaacctgc cctccagcac ctcgtacaaa taccgcgtgc gctctctgaa tgtggccgga
     4201 gccagcggca attccaacgc agtctccgtc accaccctgc tcgaaggctc gaacgccgcg
     4261 cccacgctcg atgtcattgc cgacttaggg gacatcgccg cggacagcgg cgagatcacc
     4321 gtgccgctca cgggcgtcag cgacggcggc gagttcaacc agttcgtgga catcaccgcc
     4381 agctccgaca atcccgcgct gctgccgctg ctggaggtgg actattttga ccccgaagac
     4441 tccggcctgc tgcgcttcac tcccgcgccc ggccagaccg gcacggccat catcaccgtc
     4501 accgccgacg acggccaggc cgtcaacgcc atcaccacgc gcagcttcgt cgtgcgtgtg
     4561 gtgacacccg tctcggccac cgcttttgac tccgccggcg acgcggcgga ttacctcatt
     4621 ttcgccgagc aggcgtccct ggtcttcgcg ggtggcgagg gcgcggggac gccgcccggc
     4681 ggagccatga aattcgagcg cagcgcgctg cccgccgagc atgtggccct ggcgctgcgc
     4741 aggctgcgct tcgacgccac tgtcgtcccg cacgcgagca cgagcctcat gttcaacttg
     4801 cgcaacgtgc cggagatcgc cacgggcaag gataaggcgg agatcagcct cggcttcatc
     4861 agcgagcccg cgccgggcgc caagatgaag gacaccttca acaaaacgca cccgtccttg
     4921 tttgtgaagt tcaagctgga gcatgacacg gcggacctct ccaaggaccg caagctcgag
     4981 gtcgagacgg ccagcttcga cggcttcaac gagatcaaaa actccaagaa caccctcaac
     5041 gcctttttgc aggccgcaaa ctggctgcgc ctcagtttca aattcatccg caccggcttc
     5101 gacacctacc tctgctccta cgaggtgcat gactgcggcc cggacggcac ttcggagcct
     5161 gcgctgctca tgtccggcgc accggcttcg ttcacgaaca tgccgttttt caatgacagc
     5221 agcatttacg ccggcttcgt cgtcaacggt gagaagcagg gcaacacgcc cttctggctc
     5281 gacaatcatg tcattgatgt gaacaccacc gccgccgacg cgcccgtgaa tcaggccgcc
     5341 aacgacttcg tccgcaccgg cttcacgtta aactgggtcg gaggcatcct ggggcgcgag
     5401 ccgaccggct accgcgtgga gttgtgcaaa gcggaggacg gcttcgcccc cggcaccctc
     5461 attcatgcct ccggcgcggg cgggcatgac cagggttttg caatcgccga cccctgggcc
     5521 accgatttcc tcattcgcgg actccagccc ggccagtcct accgctaccg cgtgcgcgcc
     5581 atgcgcggcg ccgaagccag cgaggtgcag gactccatcc tcgccaccct gccgtcggaa
     5641 cccgccgcgc ccaccttcga ggaatggcgc gagctgcact tcgcgggtga tttggaaaat
     5701 ccgctgacgc tcgcccacgc cgacttcggc ggtcacggat tcccaaacct gctgctttac
     5761 gcgctcggtc ttgatccgcg cacgcctccg ccttccgcgc tgctgccgcg cgtgctgccg
     5821 caggacggct acctcacgct cacttatcaa cgccgggcca acctgccgga aatccaggtc
     5881 atcccggtcg gctgcggcga cccctcatcc tggtccagcg ccggtttgac caccctttcc
     5941 gtctccgagc cggatgaaaa cggcctggaa actgtcgtcg tccgcgatgc cttccccatc
     6001 gcctccaaaa accgccgctt catgcgcctg cacctcgccg agtgatcatg tttgccgacg
     6061 cccatcccgc catccatgcc tggctgacac agcgcgatca aagcgccgcc gcctggcttg
     6121 tggacaccca ccgtccgcac atcctgcgcg ccgcccgcgc ctggggcgcg ccccccgaga
     6181 tggaggagga tgccgtgcag gaggtcctgc tccgcgtctt ccgcgcgctg ccgcgctacg
     6241 aacctcgcga gccctttgcg cactggcttg gcgtcatcac ccgcaacacc tgcgccaagc
     6301 tccgccgccg ctggtgccac cgccacaagc tcagcgcctg ctttgaaaac gcggccgccg
     6361 atgtcgcgga catcgagctg ccgcacaccc gcgcgccgga ccggcagatg atgctcgccg
     6421 aacgcctcga tcaactcggg gccgctctcg gacaactctc cccgcgcgag cgcgaaatcc
     6481 tcgaacgcca gacgctggac catgaacccg ccgagaccac agccgcctcc ctggacatga
     6541 aagctggcgc cgtgcgcacc agcctctgcc gcctgcgcaa gcaactgcgc gccgcgctgg
     6601 aggagtgatt tcagctctga aaaagcatca gcctatgcaa cgaaaacgac aatatatgct
     6661 catgagaatc gtttgcagaa gtgttgagaa tcgttataaa caagccgcgt caagccatct
     6721 gaccctcccc ttttcatcat gaaaccgccc cagaaacccg cctccaaatc ccccgctccc
     6781 cagcccactt ggtggtcgcc caaggccgcg cttggccttg ccgtcggctt cgccgctgtc
     6841 gcaggctttc cctacctcgc ctccaccacg cgcctgcttg ccgagggcaa acccgtcacc
     6901 atcgccgccg tgaaggatga gaccaaaaag gacatgatga aaaaagagaa ggagcatcat
     6961 gagcacgacg ccaagaagca gcgcgagatc aaaagcatga ctgttgccgc cgatggcacg
     7021 ctctacgcgg gcggccacgc cggagtgctg cgctggaagg atggggcttg ggaaaagctg
     7081 cccggctttg aggatgaaga agccaaatcc atcgtctccg cgccggacgg ctcgctgctc
     7141 gtcgcaggca agcacgccgc ctggaaacac aaggatggag cctggagcaa agtctatgac
     7201 ggcgacaccc acagcatttc ccaggccgcc gacggcacgc tctatgtcgc cggtcccaaa
     7261 gtcggcctga agaaaaaagc ccccggcggc gagtgggaga tggtgagcga aggctttgag
     7321 gggaagtaag ccatggccag caactggaaa ctcaccaacc gcgtctggca cgccaacctg
     7381 ggcatgctat cggccatcac gctggggctg gtcgcccttt cgtgcccggt catcgcgcac
     7441 aagtgggagg ccgggaagtt tctggaagac atccattacg gcaagttcct gccgccgcag
     7501 acgcgctgga tctggattga cgggcagggt tttctgctcg ccttcctcat tgtctccggc
     7561 ctgctgatgc accgcaaatc ggtgaaaaaa gccgccagca ccgccgcgga tgatccggct
     7621 gtggcgggct cgtccgtcac ggttttcggc cttggcgccg acgcgccggt catcgcgctc
     7681 accgaggcgg cggaaaaggc cggcctgcgc gctttccg
//
LOCUS       DAOMYJ010000237         7711 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Prosthecobacter sp. isolate AMR_MDS_5629
            NODE_3157_length_7711_cov_22.145246, whole genome shotgun sequence.
ACCESSION   DAOMYJ010000237 DAOMYJ010000000
VERSION     DAOMYJ010000237.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867850
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prosthecobacter sp. (wastewater metagenome)
  ORGANISM  Prosthecobacter sp.
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Verrucomicrobiaceae; Prosthecobacter.
REFERENCE   1  (bases 1 to 7711)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7711)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.30
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:34:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,742
            CDSs (total)                      :: 4,684
            Genes (coding)                    :: 4,676
            CDSs (with protein)               :: 4,676
            Genes (RNA)                       :: 58
            tRNAs                             :: 55
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7711
                     /organism="Prosthecobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3157_length_7711_cov_22.145246"
                     /isolate="AMR_MDS_5629"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1965333"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..368
                     /locus_tag="PLP58_18610"
     CDS             <1..368
                     /locus_tag="PLP58_18610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ10601.1"
                     /translation="GGGAEQGGKEGALHAAIVRGARAASIRALCPQVSCRIGRRRTAQ
                     PLSSPQRRDDPVMIPFTLLAMSRTCAGEYSGLRRLVQAPMPGMARARVSSPSARSCAL
                     TSPGPQPKWLPPPGVSPDP"
     gene            complement(389..1672)
                     /locus_tag="PLP58_18615"
     CDS             complement(389..1672)
                     /locus_tag="PLP58_18615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009959856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HRJ10602.1"
                     /translation="MSQPASPTEITRLRDLTPHQTRSGIAAWLGWMFDGLDMHLYTLV
                     ATAFVAQLMLVPETDPSVGTNGSIIQAAFLVGWAVGGAFFGRVGDLVGRSRTLVLTIL
                     TYAVFTGLSFFAETWWQLMIFRFLAALGIGGEWAVGASLLSETWPKKWRPWIAATLQS
                     AVNCGVLLACFAGWMLAGQEPRYIFLVGVLPAFLTLWIRKAVPETEAWEQAREGQEVP
                     RISALFGAEVRSTTWRVLVICAVSLTAHWTFLFWQQKHILSLPEIASLGKAGRDSALV
                     TALMWIMIGSIIGNYIAGAFAKMMGYRKAIVLLMAAYFVSMYAAFSRQWDWETTKLQF
                     GIIGACQGVFGLFTMCLPPLFPVLLRTTGAGFCYNIGRIVAAGGTVFFGIFVKVDDFR
                     TALLYAGFLFIPAALVALLLPQEKEAAGPVGQPAD"
     gene            1792..1881
                     /locus_tag="PLP58_18620"
     tRNA            1792..1881
                     /locus_tag="PLP58_18620"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1826..1828,aa:Ser,seq:tga)
     gene            1968..3239
                     /locus_tag="PLP58_18625"
     CDS             1968..3239
                     /locus_tag="PLP58_18625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009964158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HRJ10603.1"
                     /translation="MNRRDLFQKSSALGLGAALSTLPRLRAADKAANKIKVAVVALGR
                     GMGHVQALLALPNVEIKYLAEVDPARLEKGLKVVADKQEVSCHGVKDFRSFLDDKELD
                     AVFIATPNFWHTPAAVMALQAGKHVYVEKPGSQNAGEAELLVMAAQKSGKLVQMGNQR
                     RTWMREAIEALHGGAIGTVRYGRGFYYNSRKETGALAIPAPADLDWNLWQGPVPDEPG
                     RDLKKFAHYDWHWLWHWGNGEMGNNAIHTIDILRWGIKGEYPVKVTYNGGRYFYDDPQ
                     ETPDTGNAVFDFGHAGIEWVQSSCHARAAEKPLSEVIFYGDNGTMAAGRDTWTVYDPK
                     GAEVSKGKGAGGGDPAHFGNFIEAIRGNTRLNSPIDEGQKSTMMCHLANMAYRSNTVV
                     RCDPRTGKILDNPEALKFWSRSYRAGWEIKI"
     gene            complement(3240..3764)
                     /locus_tag="PLP58_18630"
     CDS             complement(3240..3764)
                     /locus_tag="PLP58_18630"
                     /inference="COORDINATES: protein motif:HMM:NF015648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PH domain-containing protein"
                     /protein_id="HRJ10604.1"
                     /translation="MPTDDSPTPPAGPESTLWKGHSSQWSHFWFYFFCILLAGGIIAA
                     AVTTGGIAAVGLVVPFLMWIIRWWMTKCTSYELTTQRLKIDTGILNRRHDELELYRVK
                     DYAMDRPLFQRILGLGTLTMLTSDATTPSVTLKAIRDVMDVREKLRAAVQAERDRKRV
                     RELDVDGGGASLGA"
     gene            complement(3911..5080)
                     /locus_tag="PLP58_18635"
     CDS             complement(3911..5080)
                     /locus_tag="PLP58_18635"
                     /EC_number="1.1.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009960508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0030604 - 1-deoxy-D-xylulose-5-phosphate
                     reductoisomerase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070402 - NADPH binding [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HRJ10605.1"
                     /translation="MNHAAPLPPRRRVLVLGSTGSIGTSALKVWREMPERMEIVGLAA
                     NQSVETLVRQALETGVRHAAFTDEQAAAKARALLPPDVTIHEGAQGLVDLVRATGADL
                     VLIAIVGVGGLAPALAAIEDGRHLAVASKEILVMAGEAVMEAARRRGVHVLPVDSEHN
                     AIFQCLEGRGAGEVRRLLLTASGGPFRTLPASELERVTVAQALRHPTWSMGRKITIDS
                     ATLFNKGLEMIEARWLFDVPMSRVEVVVHPQSIVHSMVEFVDNSVIAQLSHSDMCFPI
                     QYAVTWPRRAPNNLRPLDFATLGSLTFEAPRVDDFPALALARHAGETGGTLPAVMNAA
                     NEVAVDAFLRERISFPAIWRIVAAVMDAHQTQKNPSLDALIEADAWARRAAAERM"
     gene            complement(5103..5999)
                     /locus_tag="PLP58_18640"
     CDS             complement(5103..5999)
                     /locus_tag="PLP58_18640"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: protein motif:HMM:NF013327.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HRJ10606.1"
                     /translation="MTPDSPAPTPSKRRVFVSRLLSTLILWAVIAAAVQWNRPWVLVL
                     LTGFFGVAGAFEYYRLLRSGDHARSFTLLGLFICLAYWATMTWWAVRQHSAAPMWLDL
                     AALTGSVHGAFLLCYRHQLEGVVTLQRIFHTVFGVVYTVIFFGFIVRLMYFKPDGNGM
                     TGLFLVIFLVMVTKFSDMGAYAFGVLFGRHKMIPHISPAKSWEGLGGAFVGSFSAAAV
                     LMLLAPHRLEPLNWAHAMVLAPVLCATAVTGDLAESVLKRCTQIKDSGHKFPGIGGIL
                     DLTDSLLFTAPVFYFYLAAITA"
     gene            complement(6144..6329)
                     /locus_tag="PLP58_18645"
     CDS             complement(6144..6329)
                     /locus_tag="PLP58_18645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ10607.1"
                     /translation="MKTAAVLIAALGCIFCAASCDSHSFESTQVLHEGMHKAHGDDHG
                     GGHGKADDAHGQKPAKH"
     gene            complement(6409..>7711)
                     /locus_tag="PLP58_18650"
     CDS             complement(6409..>7711)
                     /locus_tag="PLP58_18650"
                     /inference="COORDINATES: protein motif:HMM:TIGR00196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052855 - ADP-dependent NAD(P)H-hydrate
                     dehydratase activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HRJ10608.1"
                     /translation="VAGRELARAGWTLLARLAVEPEKMKPLPAAHWRSLRGVARITAN
                     DEITAQGPLICLDGLLGIGSSGPLRPPLAALAAEMNRLRLSHGAFTFAMDMPSGLDGD
                     TGLPAPDAVVADVTATIARAKAGLFADTAANHAGRLALVPLPSLAPFLPAFAGPELLV
                     PNLLRPWLPRRPFDFHKGQAGRVGVIAGSRGFYGAAVLACMGALRGGAGLVTLLVKED
                     SYEALARMTPPEVMVRQVRDYRKALELRLDAIALGPGIGFAHDEEILGLIHEAPMPAV
                     VDADALTLLSRTSLSPLKIAAAPRLLTPHPGEMERLCDRLKPRLETAQSFADAHPGHT
                     LLLKGARTVIATSGHPALFNTTGHPGMATGGMGDLLTGLCAALLGQGVETHRAAGLGA
                     WLCGRAAEIAALDQAQECVIPTDAAACMGRAWRELRCGDAF"
BASE COUNT         1482 a         2449 c         2571 g         1209 t
ORIGIN      
        1 acggcggcgg tgctgagcag ggcggaaagg agggcgcgct tcatgccgcc atcgtgcgcg
       61 gcgcgcgcgc cgcgtcaata cgcgctctgt gcccgcaggt ttcgtgccgc atcgggcggc
      121 ggcggactgc gcagccgctc agctcccctc agcgccgtga tgatcccgtg atgatcccgt
      181 tcaccttgct ggcgatgtcc cgcacttgcg cgggcgagta ttccgggctc cgccgactgg
      241 tccaggcacc catgccgggg atggcaagag cgcgcgtctc cagcccgtcc gccagatcct
      301 gcgcgctcac ctcccccggc ccgcaaccga agtggttgcc gcctccggga gtctcccccg
      361 acccgtgagc tattcaccgg acaggctctt agtcagccgg ctggccgact ggaccggcgg
      421 cctccttttc ctgcggcagc agcagcgcca ccagggcggc gggaatgaac aggaacccgg
      481 cataaagcag ggcggtgcgg aagtcgtcca ccttcacgaa gatgccgaaa aagacggtgc
      541 caccagccgc gacgatgcgt ccgatgttgt agcaaaagcc cgctcccgtg gtgcgcaaga
      601 gcaccgggaa aagcggcggc aggcacatgg tgaagagacc aaacactccc tggcaggcgc
      661 cgatgatgcc aaattgcagc ttcgtggtct cccagtccca ctggcgggag aaagccgcat
      721 acatgctcac aaagtaagcg gccatgagca ggacgatggc cttgcggtag cccatcattt
      781 tggcaaacgc gcccgcgatg tagttgccga tgatgctgcc gatcatgatc cacatgaggg
      841 cggtgacgag ggcgctgtct ctgcccgcct tgcccaggga ggcgatctcc ggcaggctca
      901 ggatgtgctt ttgctgccag aaaaggaagg tccagtgcgc ggtgagcgac accgcgcaaa
      961 tgacaagcac gcgccaggtg gtggagcgca cctccgcacc gaaaagcgcg ctgatgcgcg
     1021 gcacctcctg cccttcgcgc gcctgctccc atgcctcggt ctccgggacg gccttgcgaa
     1081 tccacagggt aagaaacgcg ggcagcacac cgacgaggaa aatgtagcgc ggctcctgcc
     1141 cggccagcat ccagccggcg aagcaggcca gcagcacgcc gcagttcact gcggattgca
     1201 gggtggccgc gatccacggc cgccattttt tcggccaggt ctccgagagc aatgacgccc
     1261 ccaccgccca ttccccgccg atgcccagcg cggccaggaa acggaaaatc atgagctgcc
     1321 accacgtctc cgcgaagaag gacaggccgg tgaagacggc ataagtcagg atggtcagca
     1381 cgagtgtgcg gctgcgcccg acgaggtccc ccacacgccc gaagaaagcg ccgcccaccg
     1441 cccagccgac gaggaaggcg gcctggatga tggatccgtt cgtgcccacc gaggggtccg
     1501 tctccggcac cagcatgagc tgcgccacga aggccgtggc caccagcgtg taaagatgca
     1561 tgtccagccc gtcaaacatc caccccagcc aggcggcgat gcccgagcgg gtctggtgtg
     1621 gcgtcaggtc gcgcaggcgg gtgatttcag tcggggaggc gggctggctc atggtggcgc
     1681 ggagtttttg ccagacggcc tggaatcgca agacggcatt tcagacagga accgaacttg
     1741 ccctttgcaa cctgcggcga tgcgatacta tgccgccgcg caaccgcgcc tggagaggtg
     1801 gtcgagtggt ttatggctcc ggtcttgaaa accggcgtgg ccgaaaggtc accgtgggtt
     1861 cgaatcccac cctctccgcc agcccgccgg aggaacgcag aaaagtatcg gagccggtgt
     1921 cattcacggc gatagaggcg gaggcgggca ggcgatttca tgacctcatg aatcgccgcg
     1981 accttttcca gaaatcctcc gccctcggcc tcggtgccgc cctctccacc ctgccccgcc
     2041 tgcgcgccgc cgacaaagcc gcgaacaaga tcaaggtggc tgtcgtagca ctcgggcgcg
     2101 gcatgggtca tgtgcaggcg ctgctggccc tgccaaacgt ggagatcaaa tacctggccg
     2161 aggtggaccc tgcgcgcctg gaaaaaggcc tcaaggtcgt ggccgacaag caggaggtgt
     2221 cctgccacgg cgtgaaggat ttccgcagct ttctggatga caaggagctg gacgccgtct
     2281 tcatcgccac gccgaatttc tggcacacac ccgccgccgt catggcactg caagcaggca
     2341 agcacgtcta tgtggaaaaa cccggcagcc agaacgcggg cgaggccgaa ctgctcgtca
     2401 tggccgctca aaagtcggga aagctcgtgc aaatgggcaa ccagcgccgc acctggatga
     2461 gggaggccat cgaggccctg cacggcggcg ccatcggcac ggtgcgctac ggacgcggct
     2521 tttactacaa ctcgcgcaag gagacaggcg cgctcgccat ccccgcgccc gccgatctgg
     2581 actggaatct ctggcaggga ccggtgccgg acgagccggg gcgcgacctg aaaaaattcg
     2641 cccactacga ctggcactgg ctctggcact ggggcaacgg cgagatgggc aacaatgcca
     2701 tccataccat tgacatactg cgctggggca tcaaaggcga gtatccggtg aaggtcacct
     2761 acaatggcgg ccgctacttt tatgacgacc cccaggaaac tccggacacg ggcaacgccg
     2821 tcttcgactt cggccacgcc ggcatcgaat gggtgcagag ctcctgccat gcccgcgctg
     2881 ccgagaagcc gctctcggag gtcatcttct atggtgacaa tggcacaatg gccgccggac
     2941 gcgacacctg gacggtctat gatccgaaag gcgccgaggt cagcaaaggc aagggtgctg
     3001 gcggcggtga tccggcgcac ttcggcaact tcatcgaggc catccgcggt aacacaaggc
     3061 tcaacagccc cattgatgag ggccagaaga gcaccatgat gtgccacctg gcgaacatgg
     3121 cctaccgcag caacactgtg gtgcgctgcg atccccggac aggcaaaatt ctcgacaatc
     3181 cggaggcgct gaagttctgg tccagaagct accgcgcggg ttgggagatc aaaatctgat
     3241 caagccccca acgacgcgcc gccaccgtcc acatcgagtt cgcgcacgcg cttgcggtcg
     3301 cgctcggcct gcacggcggc gcggagtttt tcacgcacgt ccatgacatc gcggatggct
     3361 ttgagggtga cggaaggcgt ggtcgcgtcg gatgtgagca tggtcagggt tccaaggcct
     3421 agaatgcgct ggaacagggg acgatccatg gcgtagtctt tcacgcggta aagctcaagc
     3481 tcgtcatggc ggcggttgag aatgccggtg tcaattttca gacgctgggt ggtcagttca
     3541 tagctggtgc atttggtcat ccaccagcgg atgatccaca tcaagaacgg caccaccaga
     3601 cccactgccg cgatgccgcc cgtcgtcaca gcggcggcaa tgatgccgcc cgcgaggagg
     3661 atgcagaaaa agtaaaacca gaagtgcgac cactgggagc tgtggccctt ccaaagagtg
     3721 ctttcaggtc cggcgggtgg tgtgggtgag tcgtcggtcg gcatgacttg atcgggttcg
     3781 ccggatgcta agccatcggg acgcggtgcc aagcggcttt cgggggaaat ttcgccccgc
     3841 aggggcgccc gcaaaacctg cgggaaaacc ggcgcgggcg ggcatcaacc ccgcgctttt
     3901 gcgggcgcgc ctacatgcgc tccgcggcgg cgcggcgcgc ccaggcgtcg gcttcgatga
     3961 gtgcatccag gcttggattc ttctgcgttt ggtgcgcgtc catgacggcg gcgacaatgc
     4021 gccagatggc ggggaatgag atgcgctccc gcaggaatgc atccacggcg acctcgttgg
     4081 cggcgttcat cacggcgggc agggtgccgc cggtctcgcc ggcgtggcgg gcgagagcga
     4141 gcgcggggaa gtcgtccacg cggggagcct cgaaagtcag cgagccaagc gtggcgaagt
     4201 cgagcggacg cagattgttc ggcgcgcggc ggggccaggt gacggcatat tggatgggga
     4261 agcacatgtc actatggctg agctgggcga tgacgctgtt gtccacaaac tcaaccatgc
     4321 tgtggacgat gctctgggga tgcacgacga cctcgacgcg gctcatgggc acgtcaaaaa
     4381 gccagcgcgc ctcgatcatc tccaggcctt tgttgaagag cgtggcgctg tctatggtga
     4441 tcttccggcc catgctccag gtcgggtgcc tcaaggcctg cgcgacggtg acgcgctcca
     4501 gttcgctcgc aggcagagtg cggaaggggc cgccgctggc ggtgagcagg aggcgccgca
     4561 cctccccggc gccgcggcct tcgaggcatt gaaaaatggc gttatgctcg ctgtccacgg
     4621 gcaggacatg aacccctctg cgccgcgcgg cctccatgac cgcctcgcca gccatgacga
     4681 ggatttcctt gctggccacg gccagatgcc tgccatcctc aatggcggca agcgcgggcg
     4741 cgagaccgcc gacaccgaca atggcgatga gcacaaggtc ggccccggtg gcgcgcacga
     4801 gatccaccag accctgcgcg ccttcatgaa tggtcacatc aggaggcagc agcgcgcgtg
     4861 ccttggcggc ggcttgctca tcggtgaatg ccgcgtgccg cacgcctgtc tcgagcgcct
     4921 gccgcacgag ggtttccacg ctttgattcg ccgcgagtcc gacaatctcc atgcgctccg
     4981 gcatctcacg ccacactttc agcgcgctcg tgccgatgct gcccgtggag ccgagcacga
     5041 ggactcggcg gcgaggaggg agcggggcgg cgtgattcat ctggctgatt tttcccctgc
     5101 catcatgcgg tgatggccgc cagataaaaa taaaacaccg gcgcggtgaa cagcagactg
     5161 tcggtgaggt cgaggatgcc gcctatgccg ggaaatttgt ggccgctgtc cttgatctgg
     5221 gtgcagcgtt tgagcacgga ctcggccagg tcgcccgtga cagctgtggc gcacaggacg
     5281 ggcgccagca ccattgcgtg ggcccagttc aaaggctcca gccggtgcgg cgccagaagc
     5341 atcagcacag ccgccgccga aaacgatccg acaaaagcgc cgccgagtcc ctcccacgac
     5401 ttggccgggc tgatgtgcgg gatcattttg tgcctgccga atagcacacc gaaagcatat
     5461 gcccccatgt cgctgaactt ggtcaccatg accaggaaga tgaccagaaa gaggccggtc
     5521 atgccattgc cgtccggctt gaaatacatc agccgcacga taaacccgaa aaagatgacg
     5581 gtgtaaacga cgccgaagac ggtgtggaaa atgcgctgca gcgtcaccac gccctcaagc
     5641 tggtgccggt agcaaagcag gaaggcccca tgcacgctgc cggtcagcgc ggccagatcc
     5701 agccacatgg gagcggcgct gtgctggcgc acagcccacc acgtcatggt ggcccagtaa
     5761 gcgaggcaga tgaagaggcc gagcagggtg aaggagcgcg cgtggtcgcc gctgcgcagg
     5821 aggcggtagt attcgaaggc gccggccacg ccgaagaatc ctgtgagcag caccagcacc
     5881 cagggccggt tccactgcac ggcggcggcg atgaccgccc ataggatgag ggtgctcagc
     5941 agcctggaaa cgaacacacg gcgcttgctg ggcgtggggg cgggtgagtc cggtgtcatg
     6001 cgtcggaagt gcgtgcgtgg aaggcgcgct atttagagca ggcggcgcgg cgggtgccaa
     6061 gctcaatttg ccacaggctc atgccggaca ggacgaaaga tcctggaaac gtgagaacgt
     6121 cccgtcgaag gacgcgaacg gtgtcaatgc ttggcaggtt tttggccgtg ggcgtcgtcc
     6181 gccttaccgt ggccgccgcc atgatcgtcg ccgtgggcct tgtgcatgcc ttcatgcagc
     6241 acctgggtgc tttcaaagga atgactgtcg cacgaggcgg cgcagaagat gcaacccagg
     6301 gcggcgataa ggacggcggc ggttttcatg aggtcttgtt tcagaggggg taatttcgcg
     6361 gcagttgata gccgcctcgc gcatggcctg caacgggctt ttttcccgtc aaaacgcatc
     6421 cccgcagcgc aattcacgcc aggcacggcc catgcatgcc gccgcgtcgg tcggaatcac
     6481 gcactcctgc gcctgatcca gcgcagcgat ttccgcggcg cgtccgcaca accaggcgcc
     6541 caaacctgcc gcgcgatgtg tttcaacacc ctggccaagc agagccgcgc acagccccgt
     6601 caacaaatcc cccatgccgc cggtggccat gccgggatgg ccggtggtgt tgaacaaggc
     6661 cggatgaccc gaggtggcga tgaccgtgcg cgcgcctttg agcaggaggg tgtggccggg
     6721 gtgcgcgtcg gcaaaggatt gcgccgtctc caggcggggt ttgagccggt cgcacagacg
     6781 ctccatttca ccgggatgcg gggtgagcag gcgcggagcc gcggcaattt ttaaaggcga
     6841 gagagaggtg cgcgacagga gtgtcaaggc gtcggcatcc accacggcgg gcatgggagc
     6901 ctcgtgaatg aggccgagga tttcctcatc gtgggcgaaa ccgatgccgg ggccgagcgc
     6961 gatggcgtcg aggcgaagtt ccagcgcctt ccggtagtcg cgcacctggc ggaccatcac
     7021 ctccggcggc gtcatgcgcg cgagagcctc ataactgtcc tccttgacca ggagcgtgac
     7081 gagccccgcc ccgccacgca gcgctcccat gcaggcgagc acggcggcgc cgtaaaagcc
     7141 gcgcgatccg gcaatgacgc cgacacgtcc cgcctggccc ttgtgaaaat caaagggccg
     7201 tctcggcagc caggggcgca gcaggtttgg cacgagcagc tccggccctg cgaaagccgg
     7261 cagaaatggc gccaacgacg gcagcggcac gagggcgagg cgtcctgcat gattcgccgc
     7321 agtgtcggcg aagaggcccg ccttggcgcg cgcgatggtt gcggtgacat cggcaacaac
     7381 ggcatccggc gcgggcagcc ccgtgtcgcc atccagaccg ctcggcatgt ccatcgcaaa
     7441 ggtgaacgcg ccgtggctga ggcgcagccg gttcatctcc gccgccagcg cggcaagcgg
     7501 cgggcgcagg ggaccgctgg agccgatgcc cagcagcccg tcgaggcaga tcaagggccc
     7561 ctgggcggtg atttcgtcat tcgccgtgat gcgcgccaca cctcgcagcg agcgccaatg
     7621 cgcggcgggc agcggtttca ttttctcagg ctccactgcc aggcgcgcca gcagcgtcca
     7681 gcccgcgcga gccagctcgc gcccggccac g
//
LOCUS       DAOMYJ010000238         7659 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Prosthecobacter sp. isolate AMR_MDS_5629
            NODE_3191_length_7659_cov_21.142425, whole genome shotgun sequence.
ACCESSION   DAOMYJ010000238 DAOMYJ010000000
VERSION     DAOMYJ010000238.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867850
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prosthecobacter sp. (wastewater metagenome)
  ORGANISM  Prosthecobacter sp.
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Verrucomicrobiaceae; Prosthecobacter.
REFERENCE   1  (bases 1 to 7659)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7659)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.30
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:34:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,742
            CDSs (total)                      :: 4,684
            Genes (coding)                    :: 4,676
            CDSs (with protein)               :: 4,676
            Genes (RNA)                       :: 58
            tRNAs                             :: 55
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7659
                     /organism="Prosthecobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3191_length_7659_cov_21.142425"
                     /isolate="AMR_MDS_5629"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1965333"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..445
                     /locus_tag="PLP58_18655"
     CDS             <1..445
                     /locus_tag="PLP58_18655"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: protein motif:HMM:NF013640.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003855 - 3-dehydroquinate dehydratase
                     activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HRJ10609.1"
                     /translation="GRMALIEPLLDLAALVDIELRSAQEMRPLVLKAQGRGVKVVGSF
                     HDFQATPGDEVLRGAVDFAQPAGLDAVKIATFLNSMDDLMRLMRLVSAPHRLRLSCMG
                     MGPLGRVSRLVLAKCGSLLNYGFLGEANAPGQWPAPRLKDILADL"
     gene            447..1496
                     /locus_tag="PLP58_18660"
     CDS             447..1496
                     /locus_tag="PLP58_18660"
                     /inference="COORDINATES: protein motif:HMM:NF012970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase"
                     /protein_id="HRJ10610.1"
                     /translation="MSLPFPKSARLALALALLPLTGCFEDPQREERLKWREEEVNAKS
                     ADIIRREMEIAKEKQVLEGLRLDLVAKEKSVEMLQKQLAEELEKTRRVRREIEIKELR
                     GPIPQISAGRAIIIDAASGETLFEKEADKRGAIASTTKLLTALLVVEAGDLDKEIIVE
                     HSDTQCAPVRLGLKAGEKYTRRQLLTAVLVKSSNDIAQVLARDNAGSVEAFVAKMNAR
                     CVELGLQDSHYVNPHGLPSTNDDEPYSTARDLAVIAKLCDQQPEIRKIVKMQSYTFKW
                     PNGRVTELSNTNRVLRNASYCDGMKTGYTNAAGYCLVASGERNGRRRIVIVLNDTEAG
                     VWRDAQALLDWALKA"
     gene            1581..2885
                     /locus_tag="PLP58_18665"
     CDS             1581..2885
                     /locus_tag="PLP58_18665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007412970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide sugar dehydrogenase"
                     /protein_id="HRJ10611.1"
                     /translation="MNSTPAPTIGIVGLGYVGLPLSLRFAQNGARVIGFDIDPAKVAS
                     LNAGQSYILHIPAEEIAAMTGSGAFAATTDFTRVSECAAVIICVPTPLVNGRDPDLSY
                     VLNTGRAIAPHLQKGQLVCLESTTYPGTTDTDLRVVLEEGSGLKAGVDFHLAFSPERE
                     DPGNPQSDVSHIPKVIGGLTPACQKRAMEIYGIAVKTLVPVESCRVAEAVKLTENIFR
                     AVNIAMVNELKVVYAAMGIDIWQVIEAAKTKPFGFMPFYPGPGLGGHCIPIDPFYLTW
                     KAREYGQETRFIELAGEVNTAMPAYVIRRCGEALASQGRALKDARVLLLGIAYKANVD
                     DDRESPGYVLWELLEAEGAHVAYHDPHVPVIRPSREHSQFAGIENTALTAGSVAGFDL
                     VLLATNHQAVDYAIVAQNARLVVDTRNALSAHMRGRENYFKA"
     gene            2988..3926
                     /locus_tag="PLP58_18670"
     CDS             2988..3926
                     /locus_tag="PLP58_18670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007417749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HRJ10612.1"
                     /translation="MKSLITGGAGFIGSHIAEALCRKGGSVIVLDDLSLGNLDNLAWK
                     KPGDDLEFVKGDINDTALLNKLMPGCDWVFHEGALPSVPRSVAQPWESNHHNLDGTLQ
                     VLIAARDAGVKRFMFASSSSIYGDSDAPSKHESLPPNPLSPYALQKYGGEKYCQLFHQ
                     LYGFETVALRYFNVFGPRQAFDSPYSGVIAKYCTAMLKGERPLVFGDGLQARDFTFVD
                     NAVSANLLAAEAPAEKAAGRFFNTAAGESISLLQLIAELNRLTGQSLEPEFHPPRVGD
                     VRNSLADISAAREALGYEVLVDWQDGLARTLDFYRP"
     gene            complement(3971..4732)
                     /locus_tag="PLP58_18675"
     CDS             complement(3971..4732)
                     /locus_tag="PLP58_18675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ10613.1"
                     /translation="METKFFVYLGAFLLVFGVIYGVHYTTVVDDARKDHQAATDQLQE
                     IRGVLQKSRDELGKKQGIIPYLTAARKVQDEIDELRAQVGTVKSEIDKTQSLFASLIK
                     RVRSEAPGTAIPQIHLQNGTLLKGAKIVRLEGRNLTIAHDGGVANITASELSDDLQYR
                     FGFSISLPGLTFDDGERKPAAIPLVTIPSSGTEAPEPKKARAPTPPQSSMSRYKDGDP
                     NLWNSVTKQELGMVYIPGQGWLRVGPKGPIPKAGK"
     gene            complement(4742..4996)
                     /locus_tag="PLP58_18680"
     CDS             complement(4742..4996)
                     /locus_tag="PLP58_18680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ10614.1"
                     /translation="MHKQIVNEEQQLRQTQAQLVAIGHLGQFNAAGSNEFAVAKANVQ
                     VRVQDKERVARELAEEKEKLEKLKTQLADYQAAQFKASRP"
     gene            complement(5175..5651)
                     /locus_tag="PLP58_18685"
     CDS             complement(5175..5651)
                     /locus_tag="PLP58_18685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ10615.1"
                     /translation="MKPLHFAWVPALLLAACAALEAPVRHTADLSSCKSFHVTTEGGD
                     PLGFAPLLAAEMKLMNVECTEGGSAAPKSADAIIAFHHDLLPSRTDRVGKLVVQARDA
                     KTGKVLATSRSDQDASLFPASNREMARLAVRNLMAATPGPRGHPRGSLWERETLLW"
     gene            5774..6073
                     /locus_tag="PLP58_18690"
     CDS             5774..6073
                     /locus_tag="PLP58_18690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012420057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3820 family protein"
                     /protein_id="HRJ10616.1"
                     /translation="MRHDLAEIGRTRMPFGKYGPANHPPHGVPIYDLPAEYLAWFARP
                     NTGFPRGRLGALLQMVYQMKVDGSDSVFDPFRRGRGGRTPLRPERKRSHDFTGEG"
     gene            6110..7219
                     /locus_tag="PLP58_18695"
     CDS             6110..7219
                     /locus_tag="PLP58_18695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ10617.1"
                     /translation="MKHILILAAALAALPTSRAALLDSPQKSGLQLKNISAIAFGKDG
                     LLIAADSATATVAAIETGDTGPLARLAAPVSDLGSVIAGGLGTSSENITVTDMAVNPA
                     SGKIYLAVQRRPDNASLILIVDATGKIAPLDLSTAIHASVSLPGGASGSKVTRVTDVA
                     FDGDRIFATGSSNEEFASRIFTIPVPVKHGATAAVFSAETYHVAHRKWETKAPIQSFI
                     PYNAKDGRYVVGAFACTPVARFRVDDLESGAQVKGVSMVELGSGNRPLDLFDYTDASG
                     KEWLVVHTQRFKDNLFGPSKFWGARVSMDYINAVEDINEKATWRDVKEKRGPDGIEIV
                     DALFGAVQVDKFGDREMAVIRDTDGRLSLEVAALP"
     gene            7300..>7659
                     /locus_tag="PLP58_18700"
     CDS             7300..>7659
                     /locus_tag="PLP58_18700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ10618.1"
                     /translation="MWHRAADLRFLLYGAPLLIGVSALVSQSTVGLHNILNPQPAVIA
                     RADGSRVELVIPPPVRAEYIVRRAAGGPPLMGRFETAADRVIFTPALPLPAGQEYHFE
                     CRAPGGPGGTHPFRLPAV"
BASE COUNT         1395 a         2762 c         2167 g         1335 t
ORIGIN      
        1 cgggcgcatg gcgctcatcg agccgctgct ggatctggcg gcgctggtgg acatcgagct
       61 gcgcagcgct caggagatgc ggccgctggt gctcaaggcg cagggcaggg gagtgaaggt
      121 ggtcggctcc ttccatgact tccaggccac tccgggtgac gaggtgttgc gcggggcggt
      181 ggacttcgcc cagccggcgg ggttggacgc ggtgaagatc gccacttttc tcaacagcat
      241 ggacgacctg atgcggctga tgcggctggt gtccgcgccg catcgtttgc gcctttcctg
      301 catgggaatg gggccgctgg ggcgcgtttc gaggttggtg cttgcaaagt gcggcagcct
      361 gctgaactac gggtttcttg gcgaggcaaa tgctcccggc cagtggcctg ccccgcgtct
      421 caaagacatc ctggccgacc tgtgacatga gccttccttt tccgaaatcc gcccgactcg
      481 cactcgcgct ggcgcttttg ccgctgacgg ggtgctttga agacccccag cgcgaggagc
      541 ggctcaaatg gcgcgaggag gaggtcaatg ccaagtcggc ggacatcatc cgcagggaga
      601 tggagatcgc caaggagaag caggtgctcg aagggctgcg gctggacctt gtggcgaagg
      661 agaaaagcgt cgagatgctc cagaagcagc tcgccgagga gctggagaaa acccggcgcg
      721 tgcggcggga gatcgagatc aaggagctgc gcggccccat cccccagatc agcgccgggc
      781 gcgccatcat catagacgcg gccagcggcg agacgctctt tgaaaaggag gccgacaagc
      841 gcggcgccat cgccagcacc acaaagctgc tgaccgccct gctcgtggtc gaggccggcg
      901 acctcgacaa agagatcatc gtcgagcaca gcgacacgca atgcgccccc gtgcgcctcg
      961 gcctcaaggc cggggaaaaa tacacccgcc gccagcttct caccgccgtg ctggtgaaaa
     1021 gctccaacga catcgcccag gtcctggcgc gcgacaacgc cggcagcgtc gaggccttcg
     1081 tcgccaagat gaacgcccgc tgcgtcgaac tcggcctgca agacagccac tacgtcaacc
     1141 cccacggcct gccctccacc aacgacgacg agccctacag caccgcgcgc gacctcgccg
     1201 tcatcgccaa actctgcgac cagcagccgg aaatccgcaa aatcgtcaaa atgcagagct
     1261 acactttcaa gtggcccaac ggccgcgtca ccgagctctc caacaccaac cgcgtcctgc
     1321 gcaacgcctc ctactgcgac ggcatgaaga ccggctacac caacgccgcc ggctactgcc
     1381 tcgtcgccag cggcgagcgc aacggccgcc gccgcatcgt catcgtcctc aacgacaccg
     1441 aggccggcgt ctggcgcgac gcccaggccc tgctggactg ggcgctgaaa gcctgacgcc
     1501 agcccccagc ccatgcccgc gcctaccctg cctagcccct caagctgcgc gccgcctccc
     1561 gccttcacac tccctcccgc ttgaactcca cccccgctcc caccatcggc atcgtcggcc
     1621 tcggctacgt cggactccct ctcagcctcc gcttcgcgca aaacggcgcc cgtgtcatcg
     1681 ggttcgacat tgaccccgcc aaagtcgcca gcctcaatgc cggccagagc tacatcctgc
     1741 acatccccgc ggaggaaatc gccgccatga ccggcagcgg cgcctttgcc gccaccacgg
     1801 acttcacccg cgtcagcgag tgcgccgccg tcatcatctg cgtgcccacc ccgctcgtga
     1861 acggccgcga ccccgacctc agctacgtgc tgaacaccgg ccgcgccatc gccccgcatc
     1921 tgcaaaaagg ccagctcgtc tgcctcgaat ccaccaccta ccccggcacc accgacaccg
     1981 acctgcgcgt cgtcctcgaa gaaggctccg gcctcaaagc cggcgtggat ttccacctcg
     2041 ccttctcccc cgagcgcgag gaccccggca accctcaaag cgacgtctcc cacatcccca
     2101 aagtcatcgg cggcctcacc cccgcctgcc aaaagcgcgc catggagatt tacggcatcg
     2161 ccgtgaagac cctcgtgccc gtcgagtcct gccgcgtggc cgaggccgtg aagctcaccg
     2221 agaacatctt ccgcgcggtg aacatcgcca tggttaacga attgaaggtc gtctatgccg
     2281 ccatgggcat agacatctgg caggtcatcg aggccgcgaa gaccaagccc ttcggcttca
     2341 tgcccttcta ccccggcccc ggcctcggcg gccactgcat ccccattgac cccttttacc
     2401 tcacctggaa agcccgcgag tacggccagg agacccgctt catcgagctc gccggcgagg
     2461 tcaataccgc catgcccgcc tatgtcatcc gccgctgcgg cgaggcgctc gccagccagg
     2521 gcagggcgct caaagacgcc cgcgtcctcc tgctcggcat cgcctacaag gccaacgtgg
     2581 acgacgaccg cgaaagcccc ggctacgtcc tctgggagct gctcgaagcc gagggcgccc
     2641 atgtcgccta ccacgacccg cacgtccccg tcatccgccc ctcgcgcgag cacagccagt
     2701 tcgccggcat cgaaaatacc gcgctcaccg ccgggagcgt cgccggcttc gatctcgtcc
     2761 tgctcgccac caatcaccag gccgtggact acgccatcgt cgcgcaaaac gcccgcctcg
     2821 tcgtggacac ccgcaacgcc ctctccgccc acatgcgggg ccgggagaac tatttcaagg
     2881 cctgacgaag ccgcggctcg aaaatcgaaa ttcgaagctc gaaattcgaa ctcagccatc
     2941 cattccactt tccgcaatcc gcattccgca atccccccca cttccccatg aaatccctca
     3001 tcaccggcgg cgccggattt atcggttccc acatcgcaga agccctctgc cgcaaaggcg
     3061 gctccgtcat cgtgctcgat gacctcagcc tcggcaacct cgacaacctc gcctggaaaa
     3121 agcccggcga tgacctcgag ttcgtcaaag gcgacatcaa cgacaccgcc ttgctcaaca
     3181 agctcatgcc cggctgcgac tgggtcttcc acgagggcgc gctgccctcc gtcccccgct
     3241 ccgtggccca gccctgggag agcaaccacc acaacctcga cggcaccctc caggtcctca
     3301 tcgccgcgcg cgacgccggt gtgaagcgct tcatgttcgc cagctcctcc tccatctacg
     3361 gcgactccga cgcgccctcc aagcacgaga gcctgccgcc caatcccctc tccccctacg
     3421 ccctccagaa atacggcggc gaaaaatact gccagctctt tcaccagctc tacggcttcg
     3481 aaaccgtcgc cctgcgttac ttcaacgtct tcggtccgcg ccaggccttt gactcgccct
     3541 actccggcgt catcgccaaa tactgcaccg ccatgctcaa aggcgagcgc cccctcgtct
     3601 ttggcgacgg cctccaggcg cgcgacttca cctttgtgga caatgccgtg tccgccaacc
     3661 tgctcgccgc cgaggccccc gctgagaaag ccgccgggcg cttcttcaac acggccgccg
     3721 gcgagtccat cagcctgctc cagctcatcg ccgagctgaa ccgccttacc gggcagagcc
     3781 tcgagcccga gttccacccg ccgcgcgtcg gcgatgtgcg caactccctg gccgacatct
     3841 ccgccgcccg cgaagccctc ggctacgagg tgctcgtgga ctggcaggac ggcctcgccc
     3901 gcacgcttga cttctaccgg ccttgacgaa tcgaacgcaa aaccagggca aatttgactt
     3961 caagccggaa tcacttgccg gctttcggga tcggcccttt cggtcccaca cgcagccagc
     4021 cctggccggg gatgtaaacc atgcccagct cctgcttggt cactgaattc cagagattgg
     4081 gatcgccgtc cttgtagcgg ctcatggaac tctgcggcgg ggtgggcgcc cgggcttttt
     4141 tgggctcggg cgcctcagtg ccagacgatg gaatcgtcac cagggggatg gcggcgggtt
     4201 tcctctcccc atcgtcgaaa gtcagacccg gcagcgagat gctgaaacca aaacgatact
     4261 gcaagtcgtc tgaaagctcg gaggcggtga tgttggcgac gcctccgtcg tgggcaatgg
     4321 tcagattgcg tccttccagg cggacaattt tcgcgccttt gagcaaagtg ccgttctgca
     4381 ggtggatctg tggaatcgcg gttccgggag cttccgagcg aaccctcttg atcagcgagg
     4441 cgaagaggct ttgcgtcttg tctatctcag acttcaccgt gcccacctgg gcgcgaagct
     4501 cgtctatttc atcctgcact tttcgggcgg cggtgagata gggaatgatc ccctgcttct
     4561 ttcccagttc atcccggctt ttttggagaa cacccctgat ctcctgcaat tgatcagtgg
     4621 cggcctggtg gtcctttctg gcatcgtcaa ccactgtcgt gtaatgcacg ccataaatga
     4681 ccccgaaaac gaggaggaac gcgcccagat aaacgaagaa tttggtttcc atgcgggtaa
     4741 atcacggtct gctggctttg aactgggctg cctgatagtc ggccagttgt gtcttgagct
     4801 tttcaagctt ctctttctcc tccgccagct ccctggcgac cctctccttg tcctggacac
     4861 gcacctgcac attggccttg gcgaccgcga actcgttgct gccggcggcg ttgaactggc
     4921 ctaaatggcc gattgcgaca agctgcgcct gggtctgcct gagttgctgc tcctcattca
     4981 caatttgctt gtgcatttcc atggtctcga gttgcagcgc cttgtgcctg tcgcagctag
     5041 tgacacatgc tgtcagaagg ccgtaaaata ggatgcaggc accaccgttt aatcgcatag
     5101 gcatacgtct aacgccctgc gctctggccg acaagaaaaa atccgtgagg aattaacaaa
     5161 tccttccgaa cggatcacca cagcaacgtc tccctctccc agagtgaacc tcgcggatgg
     5221 ccccggggtc ccggagtggc ggccatcaga ttacggaccg cgaggcgggc catttctcga
     5281 ttggatgcag ggaaaagcga cgcatcctgg tcactgcggc tcgtggcgag caccttgccc
     5341 gttttggcgt ccctggcctg gaccacaagc ttgcccaccc ggtccgtgcg gctcggcagc
     5401 aggtcatgat gaaaggctat aatggcgtcc gcgctcttgg gcgccgccga accgccttca
     5461 gtgcattcga cattcatcag cttcatttcc gccgccagca gcggggcaaa gccgagaggg
     5521 tcgccgcctt ccgtggtcac atgaaaactc ttgcaggagg aaaggtcggc ggtgtgccgc
     5581 acgggcgcct caagcgcggc gcaggctgcg agaagcaaag cgggaaccca ggcaaagtgg
     5641 agaggtttca tggcacggta tgtaacgcct catccgtttc ttgcaagcgt gcttcccgcg
     5701 gcaaaggccg cgctgccttt gactttcctc ccgtccgcgc ctctctgcga tgatggacga
     5761 cctggccgag cgcatgcgac acgatcttgc cgagatcggc cgcacgcgca tgccgttcgg
     5821 caaatacgga cccgccaatc acccgccgca cggcgtgccc atttacgatc tgcctgccga
     5881 gtacctggcc tggtttgcgc gtcccaacac tggctttccg cgcggaaggc tgggcgcttt
     5941 gctccagatg gtttatcaga tgaaagtgga cggctccgac agtgtcttcg atcccttccg
     6001 ccgcgggcgt ggaggacgca cacccctgcg cccggaacgc aagcgcagcc acgacttcac
     6061 cggcgaggga tagttcaccc ggctccccgc cgttttgtcg gcgaacacca tgaaacacat
     6121 cctcattctt gccgcggccc tcgccgcact ccccacctcc cgcgccgcgc tgcttgactc
     6181 gcctcagaag tccggcctcc agctcaaaaa catcagcgcc atcgcctttg gcaaagacgg
     6241 cctgctcatc gcggcggact ccgccactgc cactgtggca gcgattgaga ccggcgacac
     6301 cggccccttg gcccgcttgg ccgccccagt gtccgatctc ggctctgtca tcgccggcgg
     6361 actgggaacc agctcggaaa acatcaccgt caccgacatg gcggtgaatc ccgccagcgg
     6421 aaaaatctac ctcgccgtcc agcgccgtcc ggacaatgcc agcctcatcc tcattgtgga
     6481 cgccaccggc aagatcgcgc cgctcgatct ttccaccgcg atccacgctt ccgtctccct
     6541 gcccggcgga gcttccggca gcaaggtcac gcgggtcacc gacgtggcgt tcgacggcga
     6601 ccgcattttt gccaccggtt ccagcaacga ggagttcgcc agccgcatct tcaccatccc
     6661 cgtccccgtc aaacatggcg ccaccgccgc cgtgttcagc gccgagacct accatgtggc
     6721 gcaccgcaaa tgggagacca aggcgccgat ccagagcttc atcccttaca atgccaagga
     6781 cgggcgctac gtcgtcggcg ctttcgcatg cactccggtg gcgcgcttcc gcgtggacga
     6841 tctggaaagc ggcgcgcagg tgaagggcgt cagcatggtc gaactcggca gcggcaaccg
     6901 cccgctcgat ctcttcgact acaccgacgc ctcaggcaaa gagtggctcg tcgtccacac
     6961 ccagcgcttc aaggacaacc tcttcggccc ctccaagttc tggggcgcgc gcgtcagcat
     7021 ggactacatc aacgccgtcg aggacatcaa cgaaaaggcc acctggcgcg acgtgaagga
     7081 aaaacgcggc cctgacggca tcgagatcgt ggacgcgctc ttcggcgcgg tgcaggtgga
     7141 caaatttggc gaccgcgaga tggccgtcat cagggacacc gatggccgtc tcagcctcga
     7201 agtggccgcg ctgccttgat gcgcggtctt tgccgcggag cctgcgccgg ctccgcccga
     7261 agacctcact ccaacgtcca tcccgcacat tgatttgcca tgtggcaccg tgccgccgac
     7321 ctgcgcttcc tgctctacgg cgcgccgttg ctcattggcg taagcgcgct ggtaagtcag
     7381 agcacggtcg gccttcacaa tatcctcaac ccgcagccgg cggtgattgc ccgcgctgac
     7441 ggctcccggg tcgagctggt catcccgcct ccggtccgcg ccgagtacat cgtgcgccgc
     7501 gccgcaggcg ggccgccgct catggggagg ttcgaaactg ccgccgaccg cgtcatcttc
     7561 acccccgccc tgcccctgcc cgccgggcag gaataccatt tcgagtgccg cgcccccggc
     7621 ggccccggcg gcacgcaccc cttccgcttg cccgccgtc
//
LOCUS       DAOMYJ010000239         7617 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Prosthecobacter sp. isolate AMR_MDS_5629
            NODE_3219_length_7617_cov_21.689368, whole genome shotgun sequence.
ACCESSION   DAOMYJ010000239 DAOMYJ010000000
VERSION     DAOMYJ010000239.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867850
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prosthecobacter sp. (wastewater metagenome)
  ORGANISM  Prosthecobacter sp.
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Verrucomicrobiaceae; Prosthecobacter.
REFERENCE   1  (bases 1 to 7617)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7617)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.30
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:34:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,742
            CDSs (total)                      :: 4,684
            Genes (coding)                    :: 4,676
            CDSs (with protein)               :: 4,676
            Genes (RNA)                       :: 58
            tRNAs                             :: 55
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7617
                     /organism="Prosthecobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3219_length_7617_cov_21.689368"
                     /isolate="AMR_MDS_5629"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1965333"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(241..1566)
                     /locus_tag="PLP58_18705"
     CDS             complement(241..1566)
                     /locus_tag="PLP58_18705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009963899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OFA family MFS transporter"
                     /protein_id="HRJ10619.1"
                     /translation="MSILSREATVAAPGFSRWLVPPAAVAVHMCIGQVYAFSVFNKPL
                     GAELGQDVFWVNIAYQIALAMLGISAAVFGKWVERSGPRKTMLASCLCFCGGLLLTAL
                     GVKLKSLWLIWAGYGFVGGIGLGLGYIAPVSTLVKWFPDRPGMATGMAIMGFGGGALI
                     GSPLAQELMTRFARAPDTGAMSALMTMSALYACFMIFGAIIVRVPAADWKPEGYEPKA
                     AASHGMITTASVSVDTAWKTPQFWLLWTVLCMNVSAGIGVLGRAADMCQDMFGVSVAI
                     GAGFTGLLSIFNMGGRFVWSSISDLTGRRTVYCIYFILGAALYALLPYTQQTQNRTLF
                     VVCTALIISMYGGGFATIPAYLKDMFGTYQVGAIHGRLITSWSMAAVIGPLLINGLYY
                     SRVEAGVPKSEAYNNTFYLMCGLLAVGLVANLLVRPVNPKHHILEAPKA"
     gene            1676..2773
                     /locus_tag="PLP58_18710"
     CDS             1676..2773
                     /locus_tag="PLP58_18710"
                     /inference="COORDINATES: protein motif:HMM:TIGR01420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilT/PilU family type 4a pilus ATPase"
                     /protein_id="HRJ10620.1"
                     /translation="MHQYDLIEYLALAIQAGASDLHLSPGAAPVMRLHGKMTPVTEEV
                     LDANDVRELVLGALKDTQRARLEQELELDFAIEVDHLGRFRGNACYCMGRIEGNFRFI
                     PDSIPELADLGHGPTVQGMCGLRDGLILVTGVSNSGKTTTLCSMTQAIARSRSCNIVT
                     IEDPVEFVFHHGSSIIRQRQVGFDTHEFARAMKSALRQDVDVITVSELRDLETMRTAI
                     TAAETGHLVISTLHTTDVPSTITRILDSYPKDQQEYVAAQLSHCLKGVLCQHLIRRAD
                     GSGRVMASEVMMVNSAIAGCIRAQRMHQMPSLIQIGGAQGMHTIDDSLLHLLTHGFIT
                     LEDATAHARDPAFIESGYEKVRQAQAGGKRR"
     gene            2894..3238
                     /locus_tag="PLP58_18715"
     CDS             2894..3238
                     /locus_tag="PLP58_18715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ10621.1"
                     /translation="MKPSTPALALVLSCVFTLCSCGTRFEPRSMGSPGDQLSFHRNPV
                     PARAIRTTHDAYSLGQDYGNWNASAWRGYNPWRYRNHLRLHPSMWPSFVDGYKAGFRR
                     GKEERTWVPQSP"
     gene            complement(3281..3733)
                     /locus_tag="PLP58_18720"
     CDS             complement(3281..3733)
                     /locus_tag="PLP58_18720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018969306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GatB/YqeY domain-containing protein"
                     /protein_id="HRJ10622.1"
                     /translation="MPLSTQISEDLKNAMRARDAAALNVIRALKSAVKYAAIEKLGAD
                     GELDDAGIMAVVRKEIKKRQDSIASYEQAGRLELAGTEKAEIAVLEKYLPAAMSAEEL
                     SALVSSVITELGATSKKDMGAVMKALQERAAGRADSRTLSAEVTKRLN"
     gene            3904..4698
                     /locus_tag="PLP58_18725"
     CDS             3904..4698
                     /locus_tag="PLP58_18725"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009964583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0050380 - undecaprenyl-diphosphatase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HRJ10623.1"
                     /translation="MPDWLAVIILGIIEGVTEFLPISSTGHLLIPQNLGWLEPRGDLF
                     NVVIQSGAVLAVLAVFAGRVRALATTLGEPETRDYLLKLLGAFVVTGLGGLLIKESGV
                     QLPEAVTPVALATLIGGVLIFIIEHLHKGRPGAARITWAVVVLVGLAQVLAAVFPGTS
                     RSGASILMALSMGVARPAATEFSFLLGVPTLMAAGAYKIFTACQDGGFAGENVPLLLL
                     GTAVSAVSAFIVVKWLIRFVQSHTFNGFALYRVLLGTALLAYAAGL"
     gene            4743..5312
                     /locus_tag="PLP58_18730"
     CDS             4743..5312
                     /locus_tag="PLP58_18730"
                     /inference="COORDINATES: protein motif:HMM:NF021363.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2062 domain-containing protein"
                     /protein_id="HRJ10624.1"
                     /translation="MIDHARGVFRRSMFRTYRFLKHPRKLKSNAAMRWFARHFLDKRV
                     WRPTQHTLAGGAAVGLFICMQFLPVQMPFAVIVAAIFRVNIPIALAMCWLTNPLTMPP
                     LIPLEYAVGKWVLSWFSAVPATPFPTELPESLAGTWLALKEHTPVMFFGGVIIGGLLA
                     PAGYIITWMSWDKLHHLRQRKTARRAAGE"
     gene            complement(5361..5636)
                     /locus_tag="PLP58_18735"
     CDS             complement(5361..5636)
                     /locus_tag="PLP58_18735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009961177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HRJ10625.1"
                     /translation="MTAKQVLYTGRVQGVGFRYSVKQLASGYEVTGSIRNLPDGRVEL
                     RAMSRDPGELSDFLAAIQESSLGSHIRETETSAIPPVTGQRGFVIER"
     gene            complement(5633..7111)
                     /locus_tag="PLP58_18740"
     CDS             complement(5633..7111)
                     /locus_tag="PLP58_18740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ10626.1"
                     /translation="MPKSSPAILLLVLTLAGAGAGVWLATKPVNQSPLPAGTLPAIAQ
                     NSPAEDLPAQIRLLEGQIEYLQGQNAALEEENEQLRRRIAAAGGKGMPKMDAATGGTA
                     PDFEGLTLDLMRHRQLKALPLPTTPATLRDVEDRILAWLRARQPGDEALRFALALTAL
                     GWIEQPVDPLPLRAALLARQLGGWFDTRTGLLHLVEADPASGQPAPDHPLAIAFGQVL
                     REYGATLFPPGRPLSTDALLARESLLAGDAGLTRFLLSLQNTAAQAPPSIPGEDPDHP
                     LNQVPMPVFLKELAFFPFHRGFEFAQSLHSADGFPQLDAAYSRPPASSAEIIQPALYL
                     ESPPVSADAAPASARPAAPELEGSAPYWDDSLGRFAAFTALRAHNSDDTAGAAAKGLV
                     SDRLLAYAAPEHPRDHAVWHTTLTDPAAADAFFKAMRAALISRYDVKAATDAPEKLIL
                     HDRNRRIHLSRAGAQVLLVDAAGENAARALQAAFPAPKEGAP"
     gene            7204..>7617
                     /locus_tag="PLP58_18745"
     CDS             7204..>7617
                     /locus_tag="PLP58_18745"
                     /inference="COORDINATES: protein motif:HMM:NF012383.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyltransferase family protein"
                     /protein_id="HRJ10627.1"
                     /translation="MTSASDRIRQLLDAAGVARCLDGIAAAIHERWKDEPRLALVGVY
                     QRGVPFARALRQRLRDRGMEPDFGKIDITQYRDDLQTMTVLPKLEGSEITFDLEDAVV
                     ILCDEVIHTARTTRAALEELLGFGRPRCVQIAALVD"
BASE COUNT         1198 a         2451 c         2681 g         1287 t
ORIGIN      
        1 gggggcgggg caaactgacg tttgcctcta cgcgggcggc gcgggggcgc gggtagagga
       61 agacgtgagt cttccccgga tgggaggcgc gggggatggc ggagggcgag cctcaaggcg
      121 cgcggttttc ggcgggcggg gaggagaaaa gcgggcgggc tttttcgatg gatttggcga
      181 caccccaggc gagcggcgcg gcgacgatga tccagatgag gacggctttc atggcggggg
      241 tcaggctttg ggggcttcca ggatgtggtg tttgggattg acggggcgca cgagcagatt
      301 ggccaccagg ccgacggcca gcaggccgca catgaggtaa aaggtgttgt tgtaggcctc
      361 cgacttcggc acgccagcct cgacacggct gtagtagagg ccattgatca ggagcgggcc
      421 gatgacggcg gccatggacc aggaagtgat gagccgcccg tggatggctc cgacctggta
      481 agtgccaaac atgtccttca aataggccgg gatggtggca aagccgccgc catacatgct
      541 gatgatcagc gccgtgcaga cgacgaagag cgtgcggttc tgcgtctgct gcgtgtaggg
      601 cagcagggca tacagcgccg cgccgaggat gaagtagatg caatagacgg tcctgcgccc
      661 ggtgaggtcg gagatggagg accagacaaa gcgcccgccc atgttgaaga tgctcagcag
      721 ccctgtgaag cccgcgccga tggccacgct gacgccgaac atgtcctggc acatgtccgc
      781 ggcgcgcccc agcacgccga tgcccgcgct cacattcatg cacagcacgg tccacagcag
      841 ccagaactgc ggcgtcttcc aggcggtgtc cacggacacg ctggcggtgg tgatcatgcc
      901 atgggaggcc gccgccttcg gctcatagcc ctccggtttc caatccgccg caggcacccg
      961 cacgatgatg gcgccgaaga tcatgaaaca ggcatagagc gcggacatcg tcatcagcgc
     1021 ggacatggcc ccggtgtctg gagccctggc aaaacgagtc atcaactcct gggccaacgg
     1081 agagccgatg agcgcgccgc cgccaaagcc catgatggcc atgcccgtcg ccatgccggg
     1141 acggtccggg aaccacttca ccagcgtgga cacgggggcg atgtagccca ggccgaggcc
     1201 gatgccgccg acgaagccgt agcccgccca gatcagccag agagatttca gcttcacccc
     1261 cagcgcagtc agcagcagcc cgccgcagaa gcacaggcag gaggccagca tcgtcttgcg
     1321 cgggccggag cgctccaccc atttgccaaa cacagcggcg gagatgccca gcatcgccag
     1381 cgcgatctgg taggcgatgt tcacccaaaa cacatcctgc cccagctcgg cgcccagcgg
     1441 cttgttgaac acgctgaagg catagacctg gccgatgcac atgtggaccg cgacagcggc
     1501 cggagggacg agccagcgcg agaatccggg cgcggcgacg gtggcttcac gggagagaat
     1561 ggacatgcgg cgcggtttta tggggccggg gcgggggagg gcaagatttt tgtgggaaat
     1621 ctccgccgcc ggtgttttcc attgccggcg cgggcggcag cggctctatg gcgggatgca
     1681 ccaatacgac ctcatcgagt acctcgccct ggcgatccag gccggcgcgt ccgacctgca
     1741 tctctccccc ggagccgctc cggtgatgcg cctgcacggg aagatgacgc cggtgacgga
     1801 ggaggtgctg gacgccaacg acgtgcgcga gctggtgctc ggcgcgctga aggacaccca
     1861 gcgcgcccgg ctggagcagg agctggagct ggacttcgcc atcgaggtgg accacctcgg
     1921 gcgcttccgc gggaacgcct gctactgcat gggccgcatc gaggggaact tccgcttcat
     1981 cccggactcc atccccgaac tggccgacct ggggcacggg cccaccgtgc agggcatgtg
     2041 cggcctgcgc gacgggctca tcctcgtgac aggcgtctcg aactcaggca agacgaccac
     2101 gctttgcagc atgacgcagg ccatcgcgcg ctcgcgctcg tgcaacatcg tcaccatcga
     2161 ggacccggtg gagtttgtct tccaccacgg cagcagcatc atccgccaga ggcaggtggg
     2221 ctttgacacg cacgagttcg ccagggcgat gaaaagcgcg ctgcggcagg atgtggatgt
     2281 catcaccgtg agcgagctgc gcgacctgga gaccatgcgc acggccatca ccgcggcgga
     2341 gaccgggcac ctggtcatca gcacgctgca caccacggac gtgccgagca ccatcacgcg
     2401 catcctggac tcgtacccga aggaccagca ggagtatgtg gccgcgcagc tcagccactg
     2461 cctcaagggc gtgctctgcc agcacctcat ccgccgcgcc gacggcagcg ggcgcgtcat
     2521 ggcctccgag gtgatgatgg tgaacagcgc catcgccggc tgcatccgcg cccagcgcat
     2581 gcaccagatg ccgagcctca tccagatcgg cggcgcgcag ggcatgcaca cgattgacga
     2641 cagcctcctg cacctgctca cgcacggctt catcaccctg gaggacgcca ccgcccacgc
     2701 gcgcgacccc gccttcatcg agagcggcta cgaaaaagtc cggcaggcgc aggcgggcgg
     2761 gaagcggcgt tgaggctgat ccggcccggt gtttccgccg cttccgcctg ccgcgcgcgc
     2821 cgcgccgcgc ggcaacaaga tttttacgac gcaacgcttg cccaatcttc gaagtggagc
     2881 atctttgccg ccgatgaagc catccactcc cgccctcgct ctcgtccttt cctgcgtatt
     2941 caccctctgc tcctgcggca cgcgctttga gccgcgcagc atgggttccc ctggagacca
     3001 gctctccttc caccgcaacc ccgttccggc ccgcgccatt cgcaccacgc atgacgccta
     3061 cagtctgggc caggactacg gcaactggaa cgcctccgcc tggcgcggct acaatccctg
     3121 gcgctaccgc aaccatctgc gcctgcatcc gagcatgtgg ccctcgttcg tggatggcta
     3181 caaggccggc ttccgccgcg gcaaggagga gcgcacctgg gtgccccaat cgccgtgatc
     3241 catcccgcgg actgcattca tttccgtttc acaaagcgcc tcagttgagg cgctttgtca
     3301 cttcagcgga gagcgtccgg ctgtcggcgc gcccggcggc gcgctcctgc agcgccttca
     3361 tcaccgcgcc catgtccttt ttgctggtgg cgcccagttc ggtgatgacg ctggagacca
     3421 gcgcggaaag ctcctccgcg ctcatggcgg cgggcaggta tttctccagc acggcgatct
     3481 cggccttttc cgtgccggcc agttccagcc gtcccgcctg ctcgtagctg gcgatggagt
     3541 cctggcgttt tttgatctcc ttgcgcacca cggccatgat gcccgcgtcg tcgagctcgc
     3601 cgtccgcgcc gagcttctcg atggcggcgt atttgacggc cgacttcagg gcgcggatga
     3661 cattgagcgc cgcggcgtcc ctggcgcgca tggcgttctt gaggtcttcg gagatttggg
     3721 tggagagtgg catggtggtg gcttgttgtc gcgcgcagcc ttggctgcaa aacaccgcca
     3781 tgtccagccc gcgtgtggca tgtgaaggcg cggtcacaat tcccgccgat gccgcgcttg
     3841 caatggcgcg cgttggcttc gaaggttgcg cccgtccctc tcccgctccc cctcaaaaaa
     3901 cccatgcccg actggctcgc cgtcatcatc ctcggaatca tcgaaggcgt caccgaattc
     3961 ctgcccatct cctccacggg ccacctgctc atcccgcaaa acctgggctg gctggagccg
     4021 cgcggggatc ttttcaatgt cgtcatccag agcggcgccg tgctcgccgt gctcgccgtc
     4081 ttcgccggca gggtgcgcgc cctggccacc acgctgggcg agccggagac gcgggactac
     4141 ctgctgaagc tgctcggcgc gttcgtcgtc accggcctgg gcggcctgct catcaaggaa
     4201 tccggggttc agttgccgga ggccgtcacc ccggtggcgc tggccaccct catcggcggt
     4261 gtgctcatct ttatcatcga gcacctgcac aagggccggc ccggcgcggc ccgcatcacc
     4321 tgggcggtcg tcgtgctcgt cggtctggcg caggtgctgg cggcggtgtt tcccggcacc
     4381 tcgcgctccg gggcgagcat cctcatggcg ctttccatgg gcgtggcgcg ccccgcggcc
     4441 accgagtttt ccttcctgct gggcgtgccg accttgatgg ccgcgggcgc ctacaagatt
     4501 ttcaccgcct gccaggatgg cggcttcgcg ggggaaaatg tgccgctgct gctgctgggc
     4561 accgccgtct ccgccgtttc cgccttcatc gtcgtgaaat ggctcatccg cttcgtgcag
     4621 agccacacct tcaacggctt cgcgctctac cgcgtgctgc tggggacggc gctgctggct
     4681 tacgccgccg gcctctagct caagcgcgcc gccccgtcat gccttccgcc gtcatacatc
     4741 gcatgattga tcacgccagg ggcgtcttcc ggcgttcgat gttcaggacc taccgtttcc
     4801 tgaagcatcc gcgcaagctc aagagcaacg cggccatgcg ctggttcgcc cggcattttc
     4861 tggacaagcg cgtctggcgg cccacccagc acacgctggc gggcggcgcg gcggtggggc
     4921 tgttcatctg catgcagttc ctgccggtgc agatgccctt cgcggtgatc gtcgcggcca
     4981 ttttccgggt gaacatcccc atcgccctgg ccatgtgctg gctgaccaat cctctgacca
     5041 tgccgccgct catccctttg gagtatgccg tcggcaaatg ggtgctttcc tggttctccg
     5101 ccgtgccggc cacgcctttt cccacggagc tgcccgagtc cctggccggc acctggctgg
     5161 ctttgaagga gcacacgcct gtcatgttct tcggcggcgt catcatcggc ggcctgctgg
     5221 ccccggcggg ttacatcatc acctggatgt catgggacaa gctccaccac ttgcgccagc
     5281 ggaaaaccgc gcgccgcgct gccggtgagt aggaagccgc gctgagatca gtgtcctcat
     5341 cgctgccggg atttttttcc tcagcgctcg atgacgaagc cgcgctggcc ggtcaccggg
     5401 ggaatggcgc tggtctcggt ctcgcggatg tgggagccga ggctgctttc ctggatggcg
     5461 gcgaggaagt ccgagagttc gccggggtcg cgggacatgg cgcgcagctc gacgcggccg
     5521 tcgggcaggt tgcggatgct gccggtgact tcgtagccgg aggcgagctg cttcacgctg
     5581 tagcggaagc cgacgccctg gacgcggccg gtgtagagga cttgcttggc ggtcatggtg
     5641 cgccttcttt tggcgcgggg aaggcggctt gcaaggcgcg ggcggcattt tcaccggcgg
     5701 cgtccacgag caggacttgc gcgccggcgc ggctgaggtg gatgcgacgg ttccggtcat
     5761 gcaggatgag tttttcgggg gcgtcggtgg cggctttcac atcgtagcgg gagatgaggg
     5821 cggcgcgcat ggccttgaaa aaagcatcgg ccgcggcggg gtcggtgaga gtggtgtgcc
     5881 agacggcgtg gtcgcgcggg tgctcggggg cggcataagc gagcaggcgg tcggagacga
     5941 ggcccttggc ggcggcgccg gcggtgtcgt cgctgttgtg ggcgcgcaaa gcggtgaagg
     6001 cggcgaagcg tccgaggctg tcatcccagt agggggcgga gccttcgagt tcgggggcgg
     6061 cgggccgcgc ggacgctgga gcggcatcgg cggaaacggg cgggctttcc agatagagcg
     6121 cgggctggat gatctcggcg ctggaggcgg gagggcggga gtaggcggcg tcgagctggg
     6181 gaaaaccgtc ggcgctgtgc agggactggg cgaactcgaa gccgcgatgg aacgggaaga
     6241 aggccagctc tttgaggaag acgggcatgg ggacttgatt gagggggtgg tcggggtcct
     6301 cgccgggaat ggagggaggc gcctgggcgg cggtgttttg caggctgagc aggaagcggg
     6361 tgaggccggc gtctccggcg aggagggatt cgcgggcgag cagcgcatcg gtgctcaagg
     6421 ggcgtccggg tgggaagagg gtggcgccgt attcgcgcag cacctggccg aaggcgatgg
     6481 cgagcgggtg gtccggcgcg ggctggccgg aggcggggtc tgcttcgacg aggtggagca
     6541 ggccggtgcg ggtgtcgaac cagccgccga gttgacgggc gaggagggcg gcgcgcaggg
     6601 gcaggggatc cacgggctgc tcgatccagc caagcgcggt gagggcgagg gcgaagcgca
     6661 gggcctcgtc accgggctgg cgcgcgcgca gccaggcgag gatgcggtcc tccacatcgc
     6721 gcagggtggc cggggtggtt ggcaggggca gggctttgag ctggcggtgg cgcatgagat
     6781 cgagggtgag accctcgaag tcgggcgcgg tgccgccggt ggcggcgtcc attttgggca
     6841 tccccttgcc cccggctgcg gcgatgcggc ggcgcagttg ctcgttctcc tcctcaagcg
     6901 cggcgttctg gccctggagg tattcgatct ggccttcgag cagccggatc tgcgcgggga
     6961 ggtcctcggc gggcgagttc tgcgcgatgg cgggcagggt gccggcgggc agcggggatt
     7021 gattcaccgg cttggtggca agccagacac cggcgcccgc gccggcgagg gtgagcacga
     7081 gcaggaggat ggcgggcgag gacttgggca tcccgccacg catagccacg caacgcatcc
     7141 tgtcatgccc gcgctccgca aaaggcgttg acgctttcgc cactttgcgt tcctcatcca
     7201 agcgtgacat ccgcctccga ccgcatccgc cagctgctcg acgccgcagg cgtggcgcgc
     7261 tgcctggacg ggatcgcggc ggccattcac gagcgttgga aggacgagcc gcgcctggct
     7321 ctggtgggtg tttaccagcg cggtgtgccc tttgcccgcg ccctgcgtca gcgcctgcgt
     7381 gatcggggca tggagcccga ctttggcaaa attgacatca cccaataccg tgacgacctc
     7441 cagaccatga cggtgctgcc gaagctggag ggctccgaga tcaccttcga cctcgaagac
     7501 gcagtggtga tcctttgcga cgaggtcatc cacaccgcgc gcacaacccg cgccgcgctg
     7561 gaggagctgc tcggcttcgg ccgcccgcgc tgcgtgcaga tcgccgcgct ggtggac
//
LOCUS       DAOMYJ010000240         7588 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Prosthecobacter sp. isolate AMR_MDS_5629
            NODE_3238_length_7588_cov_20.249038, whole genome shotgun sequence.
ACCESSION   DAOMYJ010000240 DAOMYJ010000000
VERSION     DAOMYJ010000240.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867850
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prosthecobacter sp. (wastewater metagenome)
  ORGANISM  Prosthecobacter sp.
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Verrucomicrobiaceae; Prosthecobacter.
REFERENCE   1  (bases 1 to 7588)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7588)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.30
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:34:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,742
            CDSs (total)                      :: 4,684
            Genes (coding)                    :: 4,676
            CDSs (with protein)               :: 4,676
            Genes (RNA)                       :: 58
            tRNAs                             :: 55
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7588
                     /organism="Prosthecobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3238_length_7588_cov_20.249038"
                     /isolate="AMR_MDS_5629"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1965333"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1066
                     /locus_tag="PLP58_18750"
     CDS             <1..1066
                     /locus_tag="PLP58_18750"
                     /inference="COORDINATES: protein motif:HMM:NF012264.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HRJ10628.1"
                     /translation="KVFGASIPELLENLKLPEYRTRYRTRRELRGRPAGEVIPAVKAW
                     AAALDKADPRYEHHLCEALWATWAQNQPDADLLRQCLTAQKHEARAAAANVLRFNPEK
                     IANATDLLLMAARDAHGRVRLEAIVAASWLDNADGARIALEALKLPLDKWMGPVTKHI
                     LEYTLKDDIAALQAAGQLDLAGNPNAAGYLAGTFKIEDAAPSAEEAAYGPTRKLSAAE
                     QKIYALGKSVFTRDAHCATCHQPNGQGIPNIYPTLAKSDWIENDERLIKIALKGLWGP
                     IEVNGQHFDPSKGVPPMMGFGGLLNDEELAAVLSYVRQSFGNDGAFITTDQVRQVRAA
                     TAGRVNFYMTDEILKEHPLK"
     gene            complement(1189..3570)
                     /locus_tag="PLP58_18755"
     CDS             complement(1189..3570)
                     /locus_tag="PLP58_18755"
                     /inference="COORDINATES: protein motif:HMM:NF037510.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HRJ10629.1"
                     /translation="MNEAETRAELIDPALKAAGWGVVEGSRIRREYVIIPGRIEGKNK
                     RGKVLKADYILEYRNTKLAVVEAKAAGEALTEGVAQAKDYAGKLAVRYACATNGHGIY
                     RIDMETGTEGEIPCYPSPDELWSFTFAKQDAWRDRFANVPYEDKGGSHPGRYYQDIAI
                     ERVLQAIARKQDRILLTLATGTGKTFIAFQIAWKLFHARWNLSGEPTRRPRILFLADR
                     NILANQAFNAFSAFPEDALVRIKPEDIRKKGKVPKNGSLFFTIFQTFMSGPPKEDGSP
                     SPYFGEYPPDFFDFIVIDECHRGGANDESNWRGILEYFKPAAQLGLTATPKRQENADT
                     YRYFGEPVFIYSLKNGINDGFLTPFKVRQIQTTLDTYYYTPDDTVVEGEIEAGRLYEE
                     ADFNRGIEIRERERKRVEIFMSQIDQNEKTLVFCATQSHALLVRDLINQIKKSKDPGY
                     CQRVTANDGELGEQYLRDFQDNEKTIPTILTTSQKLSTGVDARNIRNIVLMRPVHSMI
                     EFKQIIGRGTRLFEGKDYFTIYDFVKAHEHFLDPEWDGEPIEPETCKTCGQSPCICEK
                     TPAKPCSVCGQSPCVCPPEPCAKCGQRPCLCRKKARVKLADGKERRIQHMTCTSFWHP
                     DGTPMSAQQFMEMLFGKLPEFFHHEEELRSIWSAPDTRKKLLLGLAEKGFGHGQLAEM
                     QKIIDAEASDIFDVLAHVAYAHPPLTREVRAAHARVYINTHFSAKQQAFLDFVLQHYI
                     TVGVEELDQEKLTPLLRLKYHAIADAVADLGQPAEIGKVFAGFQKYLYADKAA"
     gene            complement(3637..4386)
                     /locus_tag="PLP58_18760"
     CDS             complement(3637..4386)
                     /locus_tag="PLP58_18760"
                     /inference="COORDINATES: protein motif:HMM:TIGR04258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four helix bundle suffix domain-containing
                     protein"
                     /protein_id="HRJ10630.1"
                     /translation="MPELFDKHGGFRRLHTFTLATIVQLETLHFCRRFLTFDHRETDT
                     KFYDPKGRQYDQMTQAARSGRQNIIEGSERSSTSKDTEMKLTDVARASLSELRGDYEI
                     FILDRAQLPWSVHAAEARTVNAISLDAAPFTDDMVHESAKHALEQRKKYARWLDAEDP
                     VVVANAMLLIIGRALNMLKSQIEAQGKAFEETGGFSERLMTKRLEARESQKNTGPECP
                     VCSKPMRQRKSAKGDFWGCSAYPECKGTRPV"
     gene            complement(4563..5765)
                     /locus_tag="PLP58_18765"
     CDS             complement(4563..5765)
                     /locus_tag="PLP58_18765"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF013579.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HRJ10631.1"
                     /translation="MKTGWQTRSLGEVCPVTNRKPKPFAGVRNYFSTGAVGPEGEISK
                     PETVDFENRPSRAGCMPEVGDIGFARMKGTKKVILIDEGLCGSLFSTGFCFIEPSHQV
                     LPRFAFYFITSDEFQDAKDSAAGDGIMGGIKNSDAESIAIPVPPLADQQRIVGVLDEA
                     FAGLATAQAHAAQNLENARALFESHLQAVFTQRGPGWVEKTLGDVCDFLNGFAFTSGD
                     AVPESQTQLVRMGNLYGNRLDLDRSPVFYPDDFAAEYSRYVLAEGDIIMSLTGTTGKE
                     DYGFAVRIPDCGHILLMNQRIMKFDAIRQDIIDDGYLLHYLRSRCFLDVLYPTANGTR
                     QANLSSVTMKTLPLPLPFLSEQKRVVTQLDALSAETQRLESLYRQKQAALEELKKSLL
                     HQAFAGEL"
     gene            complement(5762..7222)
                     /locus_tag="PLP58_18770"
     CDS             complement(5762..7222)
                     /locus_tag="PLP58_18770"
                     /inference="COORDINATES: protein motif:HMM:NF014442.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006306 - DNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-6 DNA methylase"
                     /protein_id="HRJ10632.1"
                     /translation="MFEQAFRNIDDALRKEAGCATELDYAEQTSWLLFLKYLDGLEQD
                     KADEAALEGKIYTHLLAPPYRWETWAAPKDKDGQLDHNAALSGDDLREFVDRKLFPYL
                     HGFKQKAAGPNTLEYKIGEIFGELKNKIQSGYNLRDIIDAIDGLRFRSQTEKHELSML
                     YEEKIKRMGNAGRNGGEYYTPRPLIRAMIRVVAPQLGETIYDGACGSAGFLCEALDYL
                     RETFPKRSTAQDQALQERTFYGKEKKSLAYVIGIMNLILHGIEAPNLIHTNTLTENLA
                     DIQEKDRYDIVLANPPFGGKERPEVQQNFPIRTGETAFLFLQHFIKILRAGGRGAIVI
                     KNTFLSNTDNASVSLRRHLLESCDLHTVLDCPGGTFQGAGVKTVVLFFEKGRPTRKVW
                     YYQLDPGRNLGKTNPLNDADLSDFIDRQKDLEATAKSWSVEVSAIDTATYDLSVKNPN
                     GGETVTHRRPQEILEEIAALDAESAEVLENIRGLLA"
     gene            complement(7350..>7588)
                     /locus_tag="PLP58_18775"
     CDS             complement(7350..>7588)
                     /locus_tag="PLP58_18775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ10633.1"
                     /translation="SSAATAARGARDPWNLKALIWKIKTGLRFPKTESGFSKSGFRFL
                     ETESVFSQNKSKNPPSNFLRPGDPCQHGIRESSV"
BASE COUNT         1364 a         2290 c         2354 g         1580 t
ORIGIN      
        1 caaagtcttt ggcgcaagca tccccgaact cctggaaaat ctgaagctgc ccgagtatcg
       61 cacccgctac cgcacccgcc gcgagctgcg cggacgcccc gccggcgagg tcatccccgc
      121 cgtcaaggcc tgggccgccg cgctggacaa ggccgatccg cgctacgagc accacctctg
      181 cgaagcgctc tgggccacct gggcgcagaa ccagcccgac gccgacctgc tcaggcaatg
      241 cctcaccgcc cagaagcacg aggcccgcgc cgccgccgcc aacgtgctgc gcttcaatcc
      301 cgaaaaaatc gccaacgcca ccgacctgct tctgatggcc gcccgcgacg cacatggccg
      361 tgtccgcctc gaagccattg tcgccgcctc ctggctcgac aacgccgacg gcgcccgcat
      421 tgcactcgaa gcgctcaagc tcccgctcga caaatggatg ggccccgtca ccaagcacat
      481 cctggaatac acgctgaagg acgacatcgc cgccctgcaa gccgccgggc agctcgatct
      541 cgccggcaac cccaatgccg ccggttatct cgccggcacc ttcaagatcg aggacgccgc
      601 ccccagcgcc gaggaagccg cctacggccc gacgcgcaag ctcagcgccg ccgagcaaaa
      661 aatctacgcg ctcggcaaat ccgtcttcac ccgcgacgcc cactgcgcca cttgccatca
      721 gcccaacggc cagggcatcc ccaacatcta tcccacgctc gccaagagcg actggatcga
      781 aaacgacgag cgcctcatca agatcgccct caaaggcctc tggggtccca tcgaagtcaa
      841 cggccagcac tttgacccca gcaaaggcgt gccgcccatg atgggcttcg gcggtctgct
      901 caacgacgag gaactcgctg ccgtgctcag ctacgtccgc cagagcttcg gcaacgacgg
      961 cgccttcatc accaccgacc aagtgaggca agtccgcgcc gccaccgccg gccgcgtcaa
     1021 cttctacatg accgacgaga tcctcaaaga gcacccgctc aagtaaactg ctgggaaggc
     1081 gcgaacgcag aagtcgaatt acgaagttca gtcagttcga cattcgccgg tcacaggcga
     1141 tgccgtgttt ctccagcatc acggcgatgg cgagggagcg ttcttcagct aagcggcctt
     1201 gtcggcgtaa agatattttt ggaagcccgc gaacactttt ccgatctccg ccggctggcc
     1261 aagatcggcc acggcatcgg cgatggcgtg atacttgagc cgcagcaggg gcgtgagctt
     1321 ctcctggtcc agttcctcga caccgaccgt gatgtagtgt tgaaggacaa aatccaggaa
     1381 cgcctgctgc ttggcgctga aatgcgtgtt gatgtaaaca cgggcatggg cggcgcgaac
     1441 ctccctggtg agcggtgggt gggcatacgc cacatgagca agcacgtcga agatgtcact
     1501 ggcttcggcg tcaatgatct tctgcatctc agccagttga ccgtgtccga atcccttttc
     1561 cgccaacccc agcagcaact tcttgcgcgt gtccggcgcg ctccagatgg accgcagttc
     1621 ctcctcgtga tggaagaact ccggcagctt gccgaaaagc atttccatga actgttgggc
     1681 gctcatgggg gtgccgtcag gatgccaaaa gctcgtgcac gtcatgtgct ggatgcggcg
     1741 ctccttgccg tcggccagtt tcaccctggc ctttttccgg cacaagcacg ggcgctgacc
     1801 gcacttggcg cacggctcgg gcgggcagac gcatggcgac tggccgcaga ctgagcaggg
     1861 tttggccggc gttttttcgc agatgcacgg tgactgaccg caggtcttgc aggtctctgg
     1921 ctcaatcggc tcgccatccc attccgggtc gaggaaatgt tcgtgcgcct tcacgaagtc
     1981 gtagatcgta aaatagtcct tcccttcaaa cagccgcgtg ccgcgtccga tgatctgttt
     2041 gaattcgatc atcgaatgga ccggccgcat caatacgata ttgcgaatgt tccgagcgtc
     2101 cacgccggtc gaaagcttct gtgacgtggt gaggatggtg ggaatggtct tctcgttgtc
     2161 ctggaaatcg cgcaggtatt gctccccgag ttcgccatcg ttggcggtga cgcgctggca
     2221 atagcccggg tctttgctct tcttgatctg gttgatgagg tccctcacca gcagcgcgtg
     2281 ggattgggtg gcgcagaaca ccagcgtctt ttcgttctgg tcaatctgcg acatgaaaat
     2341 ctccacccgc ttgcgctcgc gctcccggat ttcgatgccc cggttgaagt ccgcctcctc
     2401 gtaaagcctg cccgcctcga tttcgccttc caccaccgtg tcgtcgggcg tgtagtagta
     2461 ggtgtccagg gtggtctgga tttgcctcac cttgaacggc gtcaggaagc cgtcgttgat
     2521 accatttttc agcgagtaga tgaacaccgg ctcgccgaag taacggtagg tgtcggcgtt
     2581 ctcctgtcgc ttgggcgtgg ccgtgaggcc gagttgcgcc gcaggtttga aatactccag
     2641 gatgccgcgc cagttgctct catcgttggc gccgccgcga tggcattcgt caatgacaat
     2701 gaaatcaaag aagtccggcg gatactcgcc gaaatacggc gatggcgaac catcctcctt
     2761 gggcgggccg ctcatgaacg tttggaagat ggtgaagaac aggctgccgt ttttgggcac
     2821 tttgcctttc ttgcggatgt cttccggctt gatccgcacc agcgcatcct cggggaaggc
     2881 ggagaaggcg ttgaaagcct ggttggccag gatgtttcga tccgcgagaa acaggatgcg
     2941 cggcctccgg gtcggttcgc ccgagagatt ccaccgcgcg tggaaaagct tccaggcaat
     3001 ctgaaacgcg atgaaggtct tgcccgtgcc ggtggccagg gtcagcagga tgcgatcctg
     3061 cttccgcgcg atcgcctgca gcacccgttc gatggcgatg tcctggtaat accgccccgg
     3121 atgcgaaccg cccttgtcct cgtagggcac gttggcgaac cggtctcgcc acgcatcctg
     3181 tttggcgaag gtgaaactcc acaattcatc cggtgacggg tagcacggga tttcgccctc
     3241 cgtgccggtc tccatgtcaa tgcggtaaat gccgtggccg tttgtggcgc aggcgtagcg
     3301 caccgccagc ttgcccgcat aatccttggc ctgcgccacg ccttccgtga gggcttcacc
     3361 cgccgccttg gcctccacca cggccagttt cgtgtttcgg tattcgagaa tgtaatccgc
     3421 cttcagcacc ttcccgcgct tgtttttgcc ttcgatgcgg ccgggaatga tgacgtattc
     3481 ccgccggatg cggctgcctt ccaccacgcc ccagcccgcc gccttcaggg cggggtcgat
     3541 cagttcggcg cgggtttcgg cttcgttcat gcgggtagga cttcactgac gccattgacg
     3601 ccgctgacac ggtggtcaat gcggtcaaca ctgcactcac accggccgcg tgcctttgca
     3661 ctccgggtag gcggagcagc cccagaaatc ccctttcgcg gattttcgct gccgcatggg
     3721 cttgctgcac accgggcatt ccggtccggt gttcttctgg ctttcgcggg cttcgaggcg
     3781 tttggtcatg aggcgctcgc tgaagccgcc ggtttcttca aatgccttcc cctgggcttc
     3841 gatctggctt ttgagcatgt tcagggcgcg gccgatgatg aggagcatgg cgttcgccac
     3901 caccacgggg tcttcggcat ccagccagcg ggcgtatttc ttccgctgct ccagcgcgtg
     3961 cttggcggac tcatgcacca tgtcatcggt gaacggggcg gcatccaggg agatggcgtt
     4021 cacggtgcgg gcttcggcgg catgcacaga ccacgggagc tgggcgcggt cgaggatgaa
     4081 gatctcgtag tcgccgcgca gctcgctgag gctggcgcgg gccacgtcgg tgagtttcat
     4141 ctcggtgtcc ttcgaggtgg aggagcgttc ggagccttcg atgatgttct ggcggccgct
     4201 gcgggcggcc tgggtcatct ggtcgtattg gcggcccttg gggtcgtaga atttcgtgtc
     4261 cgtctcccgg tggtcgaaag tgaggaagcg gcggcaaaag tgcagggttt cgagctgcac
     4321 gatcgtcgcc agcgtgaagg tgtgcagcct gcggaacccg ccgtgtttgt cgaacagctc
     4381 tggcatggga ggaggtgtgg agggttggtg aaggccgtca gtctggtcaa agcggtcaag
     4441 tatgtcaatg cggtcctccg gcggcacagc gggaccgcac tgaccggaat gacgtcattg
     4501 tcgtccggcg acggcgtcgc ccggcaatga ggtcagccct gtcagcggcg tcaatccggt
     4561 catcacagct ccccggcaaa ggcctggtgc agcagcgact tcttcagctc ctccagcgcc
     4621 gcctgcttct ggcggtagag gctttccagt cgctgcgttt cggcggacag ggcgtcgagc
     4681 tgggtgacga cgcgtttctg ttcgctcagg aagggcaacg gcagcgggag cgtcttcatt
     4741 gttacgctgg acaggttcgc ttggcgtgtg ccgttcgcgg tcgggtagag aacatcaaga
     4801 aagcagcggg agcgcaggta gtgcagcaga taaccgtcat cgatgatgtc ctgcctgatg
     4861 gcatcgaact tcatgatgcg ctgattcatc agcaaaatat ggccgcagtc tggaatcctg
     4921 acggcgaaac cgtaatcctc ttttccggtc gttccggtga gcgacatgat gatgtcgcct
     4981 tctgctaaga cgtatcgcga atactcggct gcgaagtcgt cggggtagaa gactggactt
     5041 cgatcaagat cgagtcggtt gccgtaaaga ttgcccatgc gcacgagctg ggtctgtgat
     5101 tcagggacag catcaccact cgtgaaagcg aatccattga ggaagtcgca tacgtcgccc
     5161 agtgtcttct ccacccaccc cgggccgcgc tgggtgaaga cggcttggag gtggctttcg
     5221 aagagggcgc gggcgttttc gaggttctgg gcggcatggg cttgggcggt ggccagcccg
     5281 gcaaacgctt cgtccagcac ccccacgatc cgctgctgat ccgccagcgg gggaacagga
     5341 atcgcgattg attcggcatc ggagttcttg attccaccca taatgccgtc gccagcggcg
     5401 ctgtcctttg catcctgaaa ctcatcagaa gtgatgaaat agaaagcaaa gcgcggaaga
     5461 acttggtggc taggctcaat gaaacagaaa cctgtggaaa aaagcgaacc gcaaagacct
     5521 tcgtcgatga ggattacttt cttcgttccc ttcatgcggg caaagcctat gtctcctacc
     5581 tccggcatgc accccgctcg actaggtcgg ttttcgaaat caacagtctc aggcttactg
     5641 atttcacctt caggaccgac agcgccagta gagaagtaat tccgaacgcc agcgaacggc
     5701 ttcggtttcc tattggtcac cgggcaaact tcgcccagcg atcttgtctg ccatcccgtc
     5761 ttcatgccag cagcccccga atgttctcca gcacctccgc gctctcggcg tccagcgcgg
     5821 cgatctcctc caggatctcc tgcgggcggc ggtgggtcac ggtttcaccg ccgttggggt
     5881 ttttcacgga gaggtcgtag gtggcggtgt cgatggccga cacttccacg ctccagctct
     5941 tcgccgtcgc ctccaagtcc ttctgccggt caatgaagtc actgaggtcc gcatcattga
     6001 gcgggttcgt cttccccagg ttccggccgg gatcgagctg gtaataccac accttccgcg
     6061 tggggcggcc tttctcaaaa aaaagcacca ccgtcttcac acccgcgccc tggaaggtgc
     6121 cgccggggca gtcgagcacg gtgtgcaggt cacagctctc cagcaggtgc cgccgcaggc
     6181 tcacggaggc gttgtcggtg ttcgagagga aggtgttctt gatgacgatg gccccgcgac
     6241 cgccggcgcg gaggattttg atgaagtgct ggaggaacag aaacgccgtc tcgccggtgc
     6301 ggatggggaa attctgctgc acctccggcc gctccttgcc gccgaagggc ggattcgcca
     6361 gcacgatgtc gtagcggtcc ttttcctgaa tgtcggcgag gttttccgtg agcgtgttcg
     6421 tgtggatgag gttcggcgcc tcgatcccgt gcaggatgag gttcatgatg ccgatgacgt
     6481 aggcgaggga cttcttttcc ttgccataga aggtgcgctc ctgcagcgcc tggtcctgcg
     6541 cggtgctgcg cttcggaaag gtctcgcgca ggtagtcgag cgcctcgcag aggaagccgg
     6601 cgctgccgca ggcaccatcg tagatcgtct caccgagctg cggtgccacc acgcggatca
     6661 tggcgcggat gagcgggcgc ggggtgtagt attcaccgcc attgcgcccg gcattgccca
     6721 tgcgcttgat cttctcctcg tagagcatgc tcagctcgtg cttctcggtc tgggagcgaa
     6781 acctcagccc gtcaatcgcg tcaatgatgt cacgcaggtt gtaaccgctc tgaatcttgt
     6841 ttttcagctc gccgaagatc tcgccgatct tgtattcgag ggtgttcggg ccggcggcct
     6901 tctgcttgaa gccatgcagg taggggaaga gcttccggtc cacgaactcg cgcaggtcat
     6961 cacccgagag ggcggcgttg tggtcgagtt gaccgtcctt gtccttgggc gcggcccagg
     7021 tctcccagcg gtagggtggg gcgaggaggt gggtgtatat cttcccttcg agcgcggctt
     7081 cgtcggcttt gtcctgctcc aggccgtcga ggtatttcag gaagagcagc caggaggtct
     7141 gctcggcgta gtccagctcg gtggcgcacc cggcctcctt gcggagcgcg tcgtcgatgt
     7201 tgcggaaggc ttgttcaaac atggggcgtt agttcagaaa gagggcggga tgtggttcga
     7261 ggcgggactt gggtggaggc atctgagggt tttccgggca atggggggag tgtcaagctg
     7321 ggatgactgg gacggcgcgg tcaggctcat caaaccgagg actcccgaat accgtgctga
     7381 cagggatctc ccgggcggag gaagttggaa ggcggatttt tggacttgtt ttgggaaaaa
     7441 acggactcgg tttccaaaaa tctgaacccg gacttggaaa aaccggactc ggtttttgga
     7501 aatctgagtc cggttttaat tttccaaatg agtgctttga ggttccaggg gtcacgtgcg
     7561 ccgcgcgctg cggtggccgc gctggagg
//
LOCUS       DAOMYJ010000241         7585 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Prosthecobacter sp. isolate AMR_MDS_5629
            NODE_3243_length_7585_cov_20.701726, whole genome shotgun sequence.
ACCESSION   DAOMYJ010000241 DAOMYJ010000000
VERSION     DAOMYJ010000241.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867850
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prosthecobacter sp. (wastewater metagenome)
  ORGANISM  Prosthecobacter sp.
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Verrucomicrobiaceae; Prosthecobacter.
REFERENCE   1  (bases 1 to 7585)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7585)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.30
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:34:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,742
            CDSs (total)                      :: 4,684
            Genes (coding)                    :: 4,676
            CDSs (with protein)               :: 4,676
            Genes (RNA)                       :: 58
            tRNAs                             :: 55
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7585
                     /organism="Prosthecobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3243_length_7585_cov_20.701726"
                     /isolate="AMR_MDS_5629"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1965333"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            287..628
                     /gene="xseB"
                     /locus_tag="PLP58_18780"
     CDS             287..628
                     /gene="xseB"
                     /locus_tag="PLP58_18780"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR01280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HRJ10634.1"
                     /translation="MSDIPGNLTFEQAMQRLDEIVERMEMEKMPLDEMVDAYEEGMKL
                     LASCRQRIETARRRVELIQAGESGQATLTDFDPAAATPPSETAKTPAPARRKPAAKPE
                     AGAESGDITLF"
     gene            638..2548
                     /gene="dxs"
                     /locus_tag="PLP58_18785"
     CDS             638..2548
                     /gene="dxs"
                     /locus_tag="PLP58_18785"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012419372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008661 - 1-deoxy-D-xylulose-5-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009240 - isopentenyl diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HRJ10635.1"
                     /translation="MDTDPLKITCPADLKALPLEKLPVLAEKIRATLIETLSLTGGHL
                     GPNLGVVELTLALHRVFDTPKDKFVFDVAHQGYVHKMLTGRWDKIHTIRQYGGLNGFL
                     LRSESEHDCYGAGHAGTALGAALGMATARDLKGTDDHVVCVAGDAAFTCGPVFEALNN
                     VSAHTQRLIIVLNDNEWSIDKNVGAIAKYFNSIATNPGFASLHDKAAKFVEFVLGGGA
                     RKLAERVENTAKGLLLPQSEGAHNRSTLFEEFGIHYYGPIDGHDLPLLIQTFEFLKTQ
                     NEPVILHILTEKGRGYKPALEDPLKFHGLGKYNVETGETASTDKPTYSQIYGRSVTDF
                     AKADPRIVAITGAMPGGTGLMCFKEEIPGRYFDVGIAEEHAALFACGMATQGLRPFLT
                     IYSTFMQRAIDMVIHDMAIQDLPVRLCMDRGGLSGDDGPTHHGLFDIAYLRGIPGLVH
                     MQPKDEDEFVDMLWTMANHDKGPIAIRYPRGNGPGVTPKASPSLLEIGKAELVAEGTD
                     VALLGLGEMFPVARAAKQMLEEKGLSVALVNPRWIKPLDGALIESLARQVKVVCTLED
                     HVLMNGFGCAVMEHLSSAGITTPVARIGWPDEFIEHGSVPVLRKKHGLTAEACVEKVL
                     AKLAAQHSKDAG"
     gene            complement(2766..3284)
                     /locus_tag="PLP58_18790"
     CDS             complement(2766..3284)
                     /locus_tag="PLP58_18790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ10636.1"
                     /translation="MPRLLSICCLTALAAGAQDLAPLPTGGAELTAPEPGIAAGFLKP
                     TKSIKPPLPASQPGMTAPAQPQVNAPTVARLRRLILLPGGLSPEAVREQIAAGGQSRQ
                     PMTWVGLEARAPVMADLARFFGADVNPETQRKLIETVRHGLGGANAPKRVELVGWLPG
                     EGVMALAVYPES"
     gene            3444..6839
                     /locus_tag="PLP58_18795"
     CDS             3444..6839
                     /locus_tag="PLP58_18795"
                     /inference="COORDINATES: protein motif:HMM:TIGR02604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HRJ10637.1"
                     /translation="MITRCIIPCLWLACAGHAADTRLVRETGPLSPEEERQKLTVPEG
                     FTVQLFASEPMINKPINMAFDARGRLWVSSTVEYPYSADKSRWSDEKGTRVKDSRDAI
                     KILEDTDGDGRADKVTDFADGLNIPTGVLPWHKPEHKAGCIAWSIPNIWYFADTDGDG
                     KADLREVLFGPLGYEKDTHGMCSSFRLGLDGWIYATHGFNNTSTFRVRGQAGTSADFT
                     DSADGVSAKSVSSADLELHSGNVFRFRPDGSAVEEWTRGQVNPFGLAWDRYGNLYSAD
                     CHSSPVYQLIRGAHYPSFGKPHDGLGFAPVMCQHTHGSTGICGIVYLDGGVWGPEWDD
                     HVFLGNVVTSRVNLDKVTFTGSTPKANERPDFVTSADPWFRPVDLQLGPDNALYIADF
                     YNRIIGHYEVPLDHPGRDRERGRIWRVVRKDIKAVATDFTRMSPREIAEEMGSPNLIR
                     RHLALQEVRRRGDKSWLTDFGRVFADDSPFPPFTPAGDAWPEGYADSEPHDFKPLPLE
                     WRKTDLRFAYTLWALSYLGERFAVASVLRHLHTGGWWVEDRDDELGGLLMAHATQIVG
                     AQEKLHPWERWALGLIFDFRGSAAGRAAVQVLQNRPDFFLQVPDREPDQIDEWRFWTF
                     VCLIELCDRMIERPADDLHLQGDTHYVHALKVLLRDWLAQPWFRHDNPLMTELKPTTD
                     KPWFLSVLQSVPTAEAAAFLLDSLMLESPPERWKHVARHHRPETLSAAVAFARLPGGQ
                     PLERLQALADGLSERGMSPPAELLAWARELAEEHLANAEAHPEASWLPVPQTEPAPVW
                     SLRTRPCADGVEARVLQSMGQGGGAEESRTGALKSRAFAAPEKLALWINGHRGPPTAG
                     AHEKNLVRVVESATGTTLAHAFPPRNDTAQRTELDLSSHVGKTVHLELVDGDAGKAYA
                     WLGITRIEPPVVRVENFEAESQRRETLQKLAILLQHSAPAALRERLAAHLPPRPAPPP
                     LPVSPELRRQLDALIAARSTAYAKAAPDKARGAQIFQTHCAACHQTGGQGGIIGPQLD
                     GIGTRGVERLCEDILDPNRNVDAHFHLHILTLQDGSTLSGFLKAEAGQVLILADAAGR
                     EQRVSKSTVVKDDAQPLSLMPPVFGQTIPEKDFTDLLGWLLEK"
     gene            6893..>7585
                     /locus_tag="PLP58_18800"
     CDS             6893..>7585
                     /locus_tag="PLP58_18800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
                     /protein_id="HRJ10638.1"
                     /translation="MPAESDPPAQLKEWFNEERYRSLARLLKQASACFDTRRFLAATL
                     EGLDERSLMQRLHQAALAVDLALPGNFRARTEVLLEIAPQVGHSFVAICLCDYVATFG
                     LDAPAFSLEALRRLTPHGSAEFAVRPFLQRDLRGTLRVMREWTADTDEHVRRLASEGS
                     RPRLPWGLRLRELVRDPGPTAPILEALRDDPALYVRKSVANHLNDITKDHPGHVLEML
                     AEWEIPRREERRW"
BASE COUNT         1366 a         2679 c         2308 g         1232 t
ORIGIN      
        1 ccatgccgcc cacaccgacc acctgatggg tttcatattg cggaagcagc tcggtcaact
       61 cctccggggt gggcagttct ctttgatcag tggcgtcgtc ctcgggacat ggagcggcgt
      121 cggagttttc catgggtatt tgcggcaggg tggtcatcct atgcctggga gcagagggat
      181 tgccagtgtg aaatgaaatt cgtcattgcc accaccggct tttaacgctt gcggcggcgc
      241 atggcgtcct gataatggcc gcccgttctt cccacccatc ccgaacatga gcgacatccc
      301 cggcaacctc accttcgaac aggccatgca gcgcctggat gagatcgtgg agcgcatgga
      361 gatggaaaaa atgcccctcg atgaaatggt ggacgcttat gaggaaggca tgaaactgct
      421 ggcctcctgc cgccagcgca tcgagaccgc gcgccgccgc gtcgaactca tccaggccgg
      481 cgaatccggg caggccacgc tcaccgattt cgaccccgcc gccgcaaccc ctccctctga
      541 aacggccaaa actcccgccc cggcccgccg caaacccgcc gccaagccag aggccggcgc
      601 cgaaagcggt gacatcaccc ttttctgacc caacctcgtg gacaccgacc cgctcaaaat
      661 cacctgcccc gccgatctca aagccctgcc gctggaaaag ctgcccgtgc tcgccgaaaa
      721 aatccgcgcc accctcatcg agaccctcag cctgacaggc ggccacctcg ggccgaacct
      781 cggcgtcgtc gagctcaccc tggccctgca cagggtcttt gacacgccga aggacaagtt
      841 cgtcttcgac gtcgcccatc aaggctacgt ccacaagatg ctcaccggcc gctgggacaa
      901 gatccacacc atccgccaat acgggggcct caatggcttc ctcctgcgca gcgagagcga
      961 gcacgactgc tacggcgccg gccacgccgg caccgccctg ggcgccgcgc tcggcatggc
     1021 caccgcgcgc gacttgaagg gcacggacga ccacgtcgtc tgcgtggcgg gtgacgccgc
     1081 cttcacctgc ggacccgtct tcgaggcgct caacaacgtc tccgcccaca cccagcgcct
     1141 catcatcgtc ctcaacgaca acgagtggtc cattgacaaa aacgtcggcg ccatcgccaa
     1201 gtacttcaac agcatcgcca cgaaccccgg cttcgccagc ctgcatgaca aggcggccaa
     1261 attcgtcgag ttcgtcctcg gcggcggcgc gcgcaagctg gccgagcgcg tggaaaacac
     1321 cgccaagggc ctcctcctcc cgcagagcga gggcgcgcac aaccgcagca cccttttcga
     1381 ggagttcggc atccattact acggccccat tgacgggcac gacctgccgc tgctcatcca
     1441 gaccttcgag ttcctcaaga cgcagaacga gcccgtcatc ctgcacatcc tcactgagaa
     1501 aggccgcggc tacaaacccg ccctcgaaga cccgctgaag ttccacggcc tcggcaaata
     1561 caacgtcgag accggcgaga ccgcctccac cgacaagccc acctactccc agatctacgg
     1621 gcgcagcgtc accgacttcg ccaaggcgga cccgcgcatc gtggccatca ccggcgccat
     1681 gcccggcggc accggcctca tgtgcttcaa agaggaaatc cccgggcgct atttcgatgt
     1741 cggcatcgcc gaggagcacg ccgccctctt tgcctgcggc atggccaccc agggcctgcg
     1801 ccccttcctc accatctact ccaccttcat gcagcgcgcc attgacatgg tcatccatga
     1861 catggccatc caggacctgc ccgtgcgcct gtgcatggac cgcggcggcc tcagcggcga
     1921 cgacggcccc acccaccacg gcctttttga catcgcctac ctgcgcggca tccccggcct
     1981 cgtccacatg cagccgaagg acgaggacga gttcgtggac atgctctgga ccatggcgaa
     2041 tcacgacaaa ggccccatcg ccatccgcta cccgcgcggc aacggccccg gcgtcacccc
     2101 caaggccagc cccagcctgc tcgagatcgg caaggccgag ctcgtggccg aaggcacgga
     2161 cgtggccctg ctcggcctcg gcgagatgtt ccccgtggcc cgggccgcga aacaaatgct
     2221 ggaggaaaaa ggcctctccg tggccctcgt gaacccgcgc tggatcaagc cgctggacgg
     2281 cgcattgatc gaaagcctgg cccgccaagt gaaagtggtc tgcaccctcg aggaccacgt
     2341 gctcatgaac ggcttcggct gcgccgtcat ggagcacctc tccagcgccg gcatcaccac
     2401 ccccgtggcg cgcatcggct ggccggacga gttcatcgag cacggcagcg tgcccgtgct
     2461 gcgcaaaaag cacggcctta ccgctgaagc ctgcgtggaa aaggtgctcg ccaaactggc
     2521 ggcgcaacac agcaaagacg cagggtgaaa aacagggcgg aggctgcgtg ccaacggctc
     2581 ggacgcaaaa gttcgcccga tgtgatgcag acactcttgt ctgctccggt taccctggca
     2641 agccggccct atccgaacag gcatgagttc tgtgtcaccc tcttgcgccc gcgtctggac
     2701 agggatggaa aaatggggcc atccatcaat ggcgcccgtg cgttgcgagc cagccgtttt
     2761 acccctcagc tttcaggata caccgccagg gccatgacgc cctctccagg cagccagccg
     2821 acgagttcca cgcgcttggg ggcattggcg ccgccgaggc cgtggcggac ggtttcgatg
     2881 agtttcctct gcgtctcagg attcacgtcg gctccaaaga agcgggccag atcagccatg
     2941 accggcgcgc gcgcctccaa gccgacccag gtcatcggct gccgggactg gccgcctgcg
     3001 gcgatctgct cgcgcacggc ctcggggctg agcccgccgg gcagcaggat gagcctgcgc
     3061 agacgcgcca cggtgggcgc gtttacctgg ggctgcgctg gcgcggtcat gccgggttga
     3121 gaggcgggca gcggcggctt gatggatttg gtgggcttca gaaatcccgc ggcaatgcca
     3181 ggctcaggag cggtcaattc cgcgccgccc gtaggcagcg gcgcaagatc ttgcgcgccg
     3241 gcggccagcg cggtcagaca gcagatggag agaagcctgg gcattttttt cgaaacagaa
     3301 gggcgcgctt gtatcaggac gcgccggttg gcacaatgaa aatttaagct caatccgccg
     3361 ggcagcgtac aggaatcttc accggctgcg ctccgcgctt gcctttggcc cttcacattg
     3421 ccttttcccg gcggacccga atcatgatta cccgttgcat cattccctgc ctgtggcttg
     3481 cctgcgcggg ccacgccgcc gatacccgcc tcgtccgcga gaccgggccg ctctctcccg
     3541 aggaggagcg ccaaaagctc accgtgcccg agggcttcac cgtgcagctc tttgccagcg
     3601 agccgatgat caacaagccg atcaacatgg ccttcgatgc tcgcggccgc ctctgggtgt
     3661 catcgacggt cgagtatccc tactccgctg acaaatcgcg ctggtcggac gagaaaggca
     3721 cgcgcgtcaa agacagccgg gatgccatca aaatcctcga agacaccgac ggcgacggcc
     3781 gcgccgacaa agtcaccgac ttcgccgacg ggctgaacat tcccaccggc gtgctgccgt
     3841 ggcacaagcc ggagcacaag gccggctgca tcgcctggag catcccaaac atctggtact
     3901 tcgccgacac ggatggcgac ggcaaagccg acctgcgcga ggtgctcttc ggcccgctcg
     3961 gctatgaaaa ggacacgcac ggcatgtgca gcagcttccg gttaggtctc gacggctgga
     4021 tctacgccac ccacggcttc aacaacacct ccaccttccg cgtccgcgga caggcgggca
     4081 catccgcaga ttttacagat tccgcagatg gggtttctgc gaaatctgtg tcatctgcgg
     4141 atctggaatt gcacagcggc aatgtgtttc gcttccgccc ggacggctcc gccgtggagg
     4201 aatggacgcg cggccaggtg aatcccttcg gcctcgcgtg ggaccgttac ggcaatctct
     4261 acagcgccga ctgccacagc tcccccgtct atcaactcat ccgcggcgcg cattacccca
     4321 gctttggcaa accgcacgac gggctcggct tcgcgcccgt catgtgccag cacacgcatg
     4381 gcagcaccgg catctgcggc atcgtttatc tcgatggcgg cgtctggggg ccggagtggg
     4441 acgatcacgt cttcctcggc aacgtcgtca cctcgcgcgt gaacctcgac aaagtcacct
     4501 tcaccggcag cacgccgaag gcgaatgagc ggcccgactt cgtcaccagc gccgacccct
     4561 ggtttcgccc cgtggatctc cagctcggcc cggacaacgc cctctacatc gccgacttct
     4621 acaaccgcat catcggccac tacgaggtgc cgctcgacca ccccggacgc gaccgcgagc
     4681 gcgggcgcat ctggcgggtg gtgaggaagg acatcaaggc agtggcgacg gatttcaccc
     4741 gcatgtcgcc gcgcgagatc gccgaagaaa tgggcagccc gaatctgatc cggcgccatc
     4801 tcgcgctcca ggaggtgcgg cgacgtggtg acaaaagctg gctaacagac ttcggccggg
     4861 tcttcgccga tgacagtccg ttcccaccct tcactccggc gggcgatgcc tggcccgaag
     4921 gctatgcgga cagcgaaccc cacgatttca aacccctgcc cctcgaatgg cggaaaactg
     4981 acctgcgctt cgcttacacg ctgtgggcct tgtcgtacct gggcgaacgc tttgccgtgg
     5041 cgagcgtgct gcgccacctg catacgggcg gctggtgggt ggaggatcga gacgacgagc
     5101 tcggcggcct gctcatggcc catgccacgc agattgtcgg tgcccaggaa aagctgcatc
     5161 cctgggaacg ctgggcactg gggctcatct tcgactttcg tggcagcgcc gccggtcgtg
     5221 cggcggtgca agtactgcag aaccggccgg atttctttct ccaggtgccg gatcgcgaac
     5281 ccgatcagat tgacgagtgg cggttctgga cgttcgtatg cctgatcgaa ctatgcgacc
     5341 gcatgatcga gcggccggcc gatgatctgc atctccaggg cgacacccat tatgtccacg
     5401 ccctgaaagt cctgctgcgc gactggctcg cccagccctg gttccgccat gacaacccgc
     5461 tgatgaccga actgaaaccg accacggaca agccctggtt tttgtccgtc ctgcagtcgg
     5521 tccccacggc ggaagccgcc gccttcctgc tcgattccct gatgctggag tcgcctccgg
     5581 aacgctggaa acatgtcgcc cgccatcacc ggcccgagac actttccgcc gccgtcgcct
     5641 tcgcgcgact gccggggggc cagcctctgg aacggctgca agccctcgcc gacggcctct
     5701 ccgagcgcgg catgtcccct cccgccgagc tgctggcctg ggcgcgcgag ttggcggagg
     5761 agcacctcgc gaatgctgaa gcgcaccccg aggcttcctg gctgccggtg ccgcagactg
     5821 agcccgcgcc cgtctggtcg ctgcgcacgc ggccctgcgc ggatggcgtg gaggcgcggg
     5881 tcttgcagag catgggccag ggcggcgggg cggaggaaag ccgcacgggc gcgctgaagt
     5941 cccgcgcctt tgccgcgccg gaaaagctcg cgctgtggat caacggccac cgaggcccgc
     6001 ccaccgccgg ggcgcatgaa aagaacctgg tgcgcgtggt cgagagcgcc accggcacga
     6061 cactcgcgca cgcctttcct ccacgcaacg acaccgccca gcgcacggag ctggacctct
     6121 cctcgcatgt gggaaaaacc gtgcatctgg agctcgtgga tggcgacgcg ggcaaagcct
     6181 acgcctggct gggcatcacg cgcattgagc cgcccgtggt ccgcgtggag aactttgaag
     6241 ccgagagcca gcggcgggaa acgctgcaaa agctggccat actcctccag cacagcgcgc
     6301 ccgcggcctt gcgcgaaagg ctcgccgccc acctgccgcc gcgccccgcg ccgcctcccc
     6361 tgcccgtctc acctgagctg cgccgtcagc tcgatgccct catcgccgcg cgcagtaccg
     6421 cctatgccaa agccgcgccg gacaaggcgc gcggcgcgca gattttccag acacactgcg
     6481 ccgcctgcca ccagaccggc ggccagggcg gcatcatcgg tccgcagctc gacggcattg
     6541 gcacgcgcgg agtggagcgc ctgtgcgagg acatcctgga cccgaaccgc aatgtggacg
     6601 cgcatttcca cctccacatc ctcaccctcc aggacggctc caccctcagc ggcttcctga
     6661 aagccgaggc cggccaggtg ctcatcctcg ccgacgccgc cgggcgcgag cagcgcgtgt
     6721 cgaaaagcac cgtggtcaaa gacgacgcgc agcccctctc cctcatgccg cccgtgttcg
     6781 ggcagacgat tccagaaaaa gacttcaccg acctgctcgg ctggctgctg gagaagtgag
     6841 cattgtttgc gcttcggact ggcggcccgg gcgggacggg ctttcttgcc ggatgcccgc
     6901 cgagtccgat ccgcccgcgc aactcaagga atggttcaat gaggagcgct accgctccct
     6961 ggcacgcctg ctcaagcagg cgtctgcatg ctttgacacg cgccgctttc tggccgccac
     7021 gctggagggg ctggacgagc gcagcctgat gcagcgactg catcaggctg cgctggccgt
     7081 tgacctcgct ctgccgggaa acttccgcgc gaggacggag gtgctgcttg agatcgcgcc
     7141 gcaggtcggg cacagcttcg tggcgatatg tctttgcgat tatgtggcca ccttcggcct
     7201 ggacgccccc gcattttcgc ttgaagccct tcgccgcctc acgccgcacg gctccgcgga
     7261 gttcgcggtg cgcccgtttt tgcagcgtga tctgcgcggc actctgcggg tgatgcggga
     7321 gtggaccgcc gacacggacg agcatgtgcg ccgtctggcc agcgagggat cacgcccgcg
     7381 cctgccgtgg gggctgcgtt tgcgggaact ggtgcgcgac ccgggtccga ccgcgcccat
     7441 ccttgaagcc ctgcgcgacg atcccgcgct ttatgtgcgc aagtcggtgg ccaaccatct
     7501 caatgacatc accaaggatc atcccggcca tgtgctcgag atgctggcgg agtgggaaat
     7561 cccgcgccgg gaggagcgcc ggtgg
//
LOCUS       DAOMYJ010000242         7409 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Prosthecobacter sp. isolate AMR_MDS_5629
            NODE_3353_length_7409_cov_17.906989, whole genome shotgun sequence.
ACCESSION   DAOMYJ010000242 DAOMYJ010000000
VERSION     DAOMYJ010000242.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867850
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prosthecobacter sp. (wastewater metagenome)
  ORGANISM  Prosthecobacter sp.
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Verrucomicrobiaceae; Prosthecobacter.
REFERENCE   1  (bases 1 to 7409)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7409)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.30
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:34:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,742
            CDSs (total)                      :: 4,684
            Genes (coding)                    :: 4,676
            CDSs (with protein)               :: 4,676
            Genes (RNA)                       :: 58
            tRNAs                             :: 55
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7409
                     /organism="Prosthecobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3353_length_7409_cov_17.906989"
                     /isolate="AMR_MDS_5629"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1965333"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..555
                     /locus_tag="PLP58_18805"
     CDS             <1..555
                     /locus_tag="PLP58_18805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009960727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ10639.1"
                     /translation="ELCARVRERPARFGCLGLHEWAMVYRQSRELVRHQSHELRLPPE
                     ALAAFVEAQTIGCSHYDAYRFFTPAARPLNTLNPTLETRPQMEQAGCLHANMDLYKWS
                     AKLLPWAGSDLIGRCFELARRARLLDMRASPYDLRALGLEPVRIETPEGRALYEQEQR
                     ALAAESAPLREALRAAAESVAASE"
     gene            complement(568..1758)
                     /locus_tag="PLP58_18810"
     CDS             complement(568..1758)
                     /locus_tag="PLP58_18810"
                     /inference="COORDINATES: protein motif:HMM:NF013696.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HRJ10640.1"
                     /translation="MNPLHAAAEHHLPECLCWLRRMVEINSFTTRVEGITALGRLTAE
                     CFAGLGFEAEFAAPANLAHGSHLFLRRGTGRPVVLVTHLDTVFPPEEEQRHAFHWQEE
                     NGRIYGPGTVDNKGGTALIWLMLQTLRDTDPALFEQTPWLVAANSAEEVVGSDFADQT
                     RARCPGGARIVLVFEGGPRDASGSHLVTARKGRLEYRLECSGRAAHAGSSFAEGVNAV
                     VELAALLPRLAALSAPGKDLTVNVANISGGTVLNRVPHEAAAELEIRAFDPEVLHQAG
                     QAVEALAGRTPGGAEIRVTRLGHTLAWPGGPDTDRLFEAWQHAGQTLGMSVLPMRRGG
                     LSDANYLHTLAPTLDGLGPAGANAHCSERSADGAKLPEYVETASFVPKAALNVLALGS
                     LLRR"
     gene            complement(1769..3295)
                     /gene="cobA"
                     /locus_tag="PLP58_18815"
     CDS             complement(1769..3295)
                     /gene="cobA"
                     /locus_tag="PLP58_18815"
                     /EC_number="2.1.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009964503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004851 - uroporphyrin-III
                     C-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0019354 - siroheme biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III C-methyltransferase"
                     /protein_id="HRJ10641.1"
                     /translation="MSTPVKKGICYLVGAGPGDPGLLTLKGKECIEAADVLVYDYLCN
                     PELLRHGKPGAERVYVGKKAKAHTQTQDQINDLIVKLTREGKTVTRLKGGDPVLFGRG
                     AEEAAELAAAGVRFEIVPGITSAIAAPAYAGIPVTHRDHASQLTIFTGHEDPTKEETS
                     LDYAKLAAADGTKVFLMGVERMEEITGEFLKHGASPATPMALVRWGTHGRQRTLVATL
                     GTMAARVKETGFKAPAVAVLGDVVTEREKLNWFENRPLFGKRIVVTRTRQQAGALSRK
                     LRELGADVIELPTIRIEEPRNLMAFGELVQDCHTYDWIVFTSPNGVDAFFAMYDKLYN
                     DARSIGGARIACIGPGTAEKVRARHLAVDLMPEKDFVAEGLVKAFKDHQNMEHVTVLW
                     VKAEETREVIANDLTALGAIVDEAVAYRTVQERDDNLEALALLKEEGADMITFTSAST
                     VDSFMDLGVPLPEGVKIASIGPVTSSAARRRGLKVDMEAKTHSIPGLVEAVEKYWAGK
                     "
     gene            complement(3374..3468)
                     /gene="ffs"
                     /locus_tag="PLP58_18820"
     ncRNA           complement(3374..3468)
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="PLP58_18820"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            3646..4941
                     /locus_tag="PLP58_18825"
     CDS             3646..4941
                     /locus_tag="PLP58_18825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ10642.1"
                     /translation="MNSPATRHAFAAMIVSLAASSACSARDAHALPDGAGMRDGTSFA
                     NALDARAARALLRAGGLKPGDRLLLGSGEYRGLELEIAVSGTEPQPVVIEGRDTGGGL
                     PQLTGAWSEKNPDQGGTAIEIAPGVRDVTVRGLRVSDCVTGMLAPPARGGGARQRLVF
                     EGITMRRVRHGYYLSDCHALRVEDCHLARFTKHGFRLEDGCEDVTLRRCTADCSEGDS
                     GWEKLTESLPFGFFANGSRARNTRLLFQDCVARNHMMPLQKGSYKNGDGFVIEEGSTQ
                     VRFLRCRAIRNQDGGFDLKSPGVRLESCVALENSRNFRIWNAGTLENCFTGMAPAGLW
                     SHAGPVTVTRSTFHTLENAASVDDDANAVITLKDCLLSQVRRAFPNNAGGRLILDESS
                     ITGGDVRLKQTAPAWQGDSDAMDSTSHPGKGWRWPGPRD"
     gene            complement(4951..6315)
                     /locus_tag="PLP58_18830"
     CDS             complement(4951..6315)
                     /locus_tag="PLP58_18830"
                     /inference="COORDINATES: protein motif:HMM:NF012234.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HRJ10643.1"
                     /translation="MKSKSPSKPKRKPAAVTVAGMLRKHFRTAALHKLTTTSRMFPVT
                     ARIDLQTALVDLIGKTCQVTHQSGVQAGYAFQTFTFSMLSLDRDNAAVIAPMETEDVD
                     MGESAPFRGLKSTLWFCILDGRRIAFLLTPVLSHGEVGGWQVEIAVLPAQDGSDPSAA
                     MLAAIDAIISQARSYRGKVISLEQRDNYSGKAGSVRVHKLRGVTREQVILPEKTLRML
                     ERNVMEFIGQRARLQDLGMSIKKGLLFYGPPGTGKTHTIHYLASRLPDHTTLLITAEQ
                     VGMLDQYMQLARFLQPAMIVLEDADLIARARTSMRNPCEESLLNKLLNEMDGLREDAA
                     LLFVLTTNRPEELESALAARPGRIDQAIEFPLPDEEGRRKLIKLYAAGIGLTERVASV
                     LVRKTKGASAAFIKELMRRAAQVMLRSGDGRELSLSQIESALDEMLFAGGSLNVRLLG
                     GRQD"
     gene            6483..6902
                     /gene="tsaE"
                     /locus_tag="PLP58_18835"
     CDS             6483..6902
                     /gene="tsaE"
                     /locus_tag="PLP58_18835"
                     /inference="COORDINATES: protein motif:HMM:NF014426.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HRJ10644.1"
                     /translation="MTLLPTPEDAFEWGRRLGASLQAGDVIALCGNLGAGKTQAVKGI
                     MAGAGSRHEASSPTFTLVHEHHDGRLPVFHLDFYRMSNAREVLSAGWDELLDEPAAVV
                     VEWADLFPELMPPRTRWFHIEILPEGGRRVMEKTPAE"
     gene            6899..>7409
                     /locus_tag="PLP58_18840"
     CDS             6899..>7409
                     /locus_tag="PLP58_18840"
                     /inference="COORDINATES: protein motif:HMM:NF013012.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ10645.1"
                     /translation="MNPVLALDLSTPRGVIAVTCGAAVLFEAAFVSERSHNARLFAPL
                     AEALAVIGERRARMVVGTGPGSYTGVRIAIAAAQGVAVSRGWTVAGLPTLACAPGCRV
                     LGDARRGFFYIAAVSAASEVGSLELVDERTAQAAVSAGGDWVTFDARAPLGLDRVRLA
                     QPDAVALARI"
BASE COUNT         1128 a         2361 c         2616 g         1304 t
ORIGIN      
        1 gagctgtgcg cgcgcgtgcg ggagcggccc gcgcgcttcg gctgcctggg cctgcatgag
       61 tgggccatgg tttaccggca gagccgcgag ctggtccggc accagagcca tgagctgcgc
      121 ctgccgccgg aggcgctggc cgcctttgtc gaggcgcaga caatcggctg ctcccactac
      181 gacgcctacc gcttcttcac ccccgccgcg cgcccgctca acacgctcaa tcccaccctc
      241 gaaacccgcc cgcagatgga gcaggccggc tgcctgcacg cgaacatgga cctctacaaa
      301 tggtccgcca aactcctgcc ctgggccggc tccgacctca tcggacgctg cttcgagctg
      361 gcccgccggg cgcgcctgct cgacatgcgc gccagcccct acgacctgcg cgccctgggc
      421 ctggagccgg tgcgcatcga gaccccggag ggccgcgcgc tctacgaaca agagcagcgc
      481 gccctggccg ccgagtccgc ccccctgcgc gaggccctgc gcgccgccgc tgagtccgtc
      541 gcggcgtcag agtgacgcag cttcagttta tctgcgcagc agcgagccaa gggccagcac
      601 gttgagggcg gctttgggga cgaaggaggc ggtctcgacg tattcgggga gcttggcgcc
      661 gtcggcgctg cgctcggagc agtgggcgtt ggcgccggcg gggccgaggc cgtcgagggt
      721 gggtgcgagg gtgtggaggt agttcgcgtc gctgaggccg ccgcgccgca tgggcaggac
      781 gctcatgccg agggtctgac ccgcatgctg ccatgcctcg aaaaggcggt cggtgtccgg
      841 tccgcctggc caggcgaggg tgtggccaag gcgcgtgacg cggatttccg cgccgccggg
      901 ggtccggcca gcgagggctt cgacggcctg cccggcctgg tgcagcactt cggggtcgaa
      961 ggcgcggatt tcgagctcgg cggcggcttc gtgcggcacg cggttgagga cggtgccgcc
     1021 gctgatgttg gcgacattga cggtgaggtc tttccccggc gcggagaggg cggcgaggcg
     1081 gggcaggagc gcggcgagtt cgacgacggc gttcaccccc tccgcgaagc tgctgccggc
     1141 atgggcggcg cgtccggagc attcgagacg gtattcgagc ctgcctttgc gcgcggtgac
     1201 gaggtggctg ccgctggcgt cgcgcgggcc gccctcaaag acaagcacga tgcgcgcgcc
     1261 cccggggcag cgcgcgcgtg tctggtcggc gaagtcggag ccgacgactt cctcggcgga
     1321 gttggcggcg acgagccacg gggtttgctc aaagagcgcg gggtcggtgt cgcgcagggt
     1381 ttgcagcatg agccagatca aggcggtgcc gcctttgttg tccacggtgc cggggccgta
     1441 aatgcggccg ttttcctcct gccagtggaa ggcgtggcgc tgctcctctt cgggggggaa
     1501 gacggtgtcg aggtgggtga cgagcacgac gggcctgccg gtgccgcgtc tgagaaagag
     1561 gtggctgccg tgggcaagat tggccggcgc ggcgaactca gcctcgaagc cgagaccggc
     1621 gaagcattcg gcggtgagcc ggccaagggc ggtgatgcct tcgacgcggg tggtgaagct
     1681 gttgatttcc accatgcgcc tcagccagca caggcactcc ggcagatggt gttcggcggc
     1741 ggcgtggagc ggattcatgc gctgcgggtc attttcccgc ccagtatttc tccacggctt
     1801 cgaccaggcc ggggatgctg tgggtctttg cctccatgtc caccttcaat ccgcggcgcc
     1861 ttgccgcgct gctggtgacg gggccgatgc tggcgatttt gacgccctcc ggcagcggca
     1921 cgccgaggtc catgaagctg tccaccgtgc tggcgctggt gaaggtgatc atgtccgcgc
     1981 cttcctcctt gagcagggca agggcttcga ggttgtcgtc cctttcctgg acggtgcggt
     2041 aggcgacggc ctcgtccacg atggcgccca gggcggtgag atcgttggcg atgacttcgc
     2101 gcgtctcctc ggccttcacc cacagaacgg tgacgtgctc catgttctgg tggtctttga
     2161 acgccttgac gaggccctcg gccacgaagt ccttctccgg catgaggtcc accgccaggt
     2221 gccgggcgcg caccttctcc gcggtgccgg ggccgatgca ggcgatgcgc gcgccgccga
     2281 tgctgcgggc gtcattgtag agcttgtcat acatggcgaa gaaggcgtcc actccgttcg
     2341 ggctggtgaa gacgatccag tcgtaggtgt ggcagtcctg cacgagctcg ccaaaggcca
     2401 tgaggttgcg cggttcctca atgcggatgg tgggcagctc gatgacatcc gcgcccagct
     2461 cgcgcagttt gcggctgagc gcgccggcct gctggcgggt gcgggtgacg acgatccttt
     2521 tgccaaagag cgggcggttt tcaaaccagt tgagcttttc ccgctcggtg acgacatcgc
     2581 cgaggacggc cacggctggc gctttgaatc ctgtctcttt gacccgcgcg gccatggtgc
     2641 cgagcgtggc cacgagcgtg cgctgacggc catgcgtgcc ccagcgcacc agggccatgg
     2701 gtgtggcggg ggaggctccg tgtttgagaa actcgcccgt gatctcctcc atgcgctcga
     2761 cgcccatgag gaaaaccttg gtgccgtcag cggcggccag cttcgcgtaa tccaggctgg
     2821 tctcctcttt tgtggggtcc tcgtggccgg tgaaaatggt tagctggctg gcgtggtcgc
     2881 ggtgcgtgac ggggatgccc gcgtaggccg gcgcggcgat ggcgctggtg atgccgggca
     2941 cgatctcgaa cctcactccc gccgcggcca gctcggcggc ctcctcggcg ccgcgcccga
     3001 aaagcacggg gtcgccgcct ttgagccggg tgacggtctt gccctcgcgg gtgagtttga
     3061 caatgaggtc gttgatttga tcctgtgtct gcgtgtgggc tttggccttc ttgcccacat
     3121 aaacgcgctc cgcgccgggt ttgccgtggc gcagcagctc gggattgcac aggtagtcat
     3181 agacgagcac atcggcggcc tcaatgcact ccttgccctt gagggtgagc aggccgggat
     3241 cgccggggcc ggcgccgacg aggtagcaga tgcctttttt tacgggggtg gacatggttt
     3301 gggcggggat gatggcgcgg cggcggcccc tggcaagagc cgcccggcgt ggggcggcat
     3361 ggatacggcg gggaagtgtc agggaaccgc ggcaaaaaca ggcatgctgc cgttgcttcc
     3421 gtcaggacct ggcggggttc gcaagctgct catccacggc ccctgacccg gcgagactag
     3481 agcggaggcc ggagcgggac aaggggcaat ctcggctttt ccgcgcggcg ggcgcgtgat
     3541 gccaggggca aggaacgcga ggagatgttt tgcgacggac ctggcaagat cacaccgaaa
     3601 gagccgcgtc caggaatccg tcatgaaccg gtgccatttc cctccatgaa ctccccggca
     3661 acccgccacg ctttcgccgc gatgattgtt tcgctggcag catcctccgc ctgttccgcc
     3721 cgtgatgcgc atgcgctgcc ggacggcgcc ggcatgcgcg atggcacgag ctttgccaac
     3781 gcgctggatg cgcgggccgc tcgcgccctg ctgcgcgcgg gcggcttgaa acccggtgac
     3841 cggctgctgc ttggcagcgg agaatatcgc gggctggaac tggagatcgc cgtctccggc
     3901 acggagccgc agcctgtggt gatcgaaggc cgggacacgg gcggcgggct gccccagctc
     3961 accggtgcct ggagcgagaa gaacccggat caaggcggca cggcgatcga gatcgcgccc
     4021 ggtgtgcggg atgtgaccgt gcgcggcttg cgcgtctcgg attgcgtcac cggcatgctg
     4081 gctcccccgg ccaggggcgg cggcgcgcgg cagcggctgg tgtttgaggg catcaccatg
     4141 cgccgtgtgc ggcatggcta ttatctgagc gactgtcacg cgctgcgcgt ggaggactgc
     4201 cacctcgcgc gcttcacgaa gcacggcttc cgactggagg acggctgcga ggacgtgacg
     4261 ctgcggcgct gcacggcgga ttgcagcgag ggggactccg ggtgggaaaa gctcaccgag
     4321 tccctgccct tcggcttctt tgccaacggc tcgcgggctc ggaacacgcg cctgctcttt
     4381 caggattgcg tggcgcgcaa ccacatgatg ccgctgcaaa agggcagcta caaaaacggc
     4441 gacggcttcg tcatcgagga gggcagcacg caggttcgtt tcctccgctg ccgcgccatc
     4501 cgcaaccagg acggaggctt tgatctgaaa tcacccggcg tgcggctgga aagctgcgtg
     4561 gcgctggaaa acagccgcaa cttccgcatc tggaacgccg gcacgctgga gaactgcttc
     4621 accggcatgg cgccggccgg gctgtggagc cacgccggcc ctgtcaccgt cacgcggagc
     4681 acctttcaca cgctggaaaa cgccgcctcc gtggatgacg acgccaacgc ggtgatcacg
     4741 ctcaaagact gcctgctcag ccaggtgcgg cgcgcctttc ccaataacgc gggaggcagg
     4801 ctcatcctgg acgagtccag catcacaggc ggcgatgtgc gtctgaaaca gaccgccccc
     4861 gcctggcagg gggacagcga cgcgatggac agcaccagcc atcctggcaa gggctggagg
     4921 tggccggggc cgcgtgactg aggggatggt tcaatcctgg cggccgccga gcagcctcac
     4981 attcaagctg cctccggcga aaagcatctc atccagcgcg gactcgatct gtgacaggct
     5041 gagctcgcgc ccgtcaccgg agcgcagcat gacctgggcg gcgcggcgca tgagttcctt
     5101 gatgaaggcg gcgctcgcac ccttcgtttt gcggaccagg acagacgcca cccgctccgt
     5161 gaggccgatt cccgccgcgt agagcttgat gagtttccgg cgtccttcct catccggcag
     5221 aggaaattcg atggcctggt ctatcctgcc tgggcgggcg gcaagggcgg actccaactc
     5281 ctcgggccgg ttcgtcgtca gcacaaaaag gagcgccgca tcctcgcgca gaccgtccat
     5341 ctcattgagc agcttgttga gcaggctctc ctcgcacgga ttgcgcatgg aggtcctggc
     5401 ccgcgcgatc aggtcggcgt cttcgagcac gatcatcgcg ggctggagaa agcgcgcgag
     5461 ctgcatatac tggtcgagca tccccacctg ctcggcggtg atgagcagcg tggtgtggtc
     5521 gggaagccgg ctcgcaaggt agtggatcgt gtgcgtcttg cccgttcccg gagggccgta
     5581 aaagagcagc cctttcttga tgctcatgcc caggtcctga agcctggctc gctgcccgat
     5641 gaactccatg acgttgcgct ccagcatccg aagggttttt tcggggagaa tgacttgttc
     5701 ccgggtcacg ccgcgcagct tgtggactct cacgctgccc gccttgccgg aatagttgtc
     5761 ccgctgctca agcgagatga cttttcccct gtatgaccgc gcctggctga tgatcgcgtc
     5821 aatcgcggcc agcatcgcgg cactgggatc cgaaccgtct tgcgcgggca gcacggcaat
     5881 ctccacctgc catccgccaa cctctccatg cgaaagaaca ggagtgagga gaaaggcgat
     5941 acggcggccg tcgaggatgc agaaccaaag cgttgactta agcccgcgaa agggcgcgct
     6001 ttcgcccatg tccacatcct ccgtctccat cggtgcgatc acggccgcat tgtcacgatc
     6061 cagcgacagc atgctgaaag tgaatgtttg gaacgcatat ccagcctgca cacccgactg
     6121 gtgcgtcacc tggcaggtct ttccgatgag atcaacaagc gccgtttgca ggtcaatccg
     6181 cgcggtgacg gggaacatgc gtgaagtcgt ggtcagcttg tgcagtgcgg ccgtgcgaaa
     6241 gtgtttacgc aacatgccgg cgacggtgac cgccgcgggc ttgcgcttcg gtttggatgg
     6301 ggatttggat ttcatggcag ggctggcgcc cggagtctag ggggcattcc ccgcgaggca
     6361 aacccgcagc gggcatcggg tcttgtggaa tgtgaaattc cgcgggacac ggcgcgctgg
     6421 agaatgttga ttccgccggt gccgcgcatg aaccctcgcc cacacgcatt ctctccattg
     6481 tcatgaccct cctgcccact ccagaggatg ccttcgaatg gggccgccgg ctcggagcct
     6541 cgttgcaggc gggcgatgtc atcgccctgt gcggaaacct cggcgcgggc aagacgcagg
     6601 cggtgaaggg catcatggcg ggcgccggtt cgcggcacga ggccagcagc cccacgttca
     6661 ccctcgttca cgagcatcac gacgggcggc tgccggtttt ccacctcgac ttttaccgca
     6721 tgagcaacgc gcgcgaggtg ctctccgccg gctgggatga gctgctggat gagccggccg
     6781 cggtggttgt cgaatgggcg gaccttttcc cggagctcat gccgccgcgg acgcgctggt
     6841 ttcacatcga gatcttgcct gagggcggca ggcgtgtgat ggagaagacg cctgccgaat
     6901 gaatcccgtc ctcgcccttg atctttccac gccgcgcgga gtcatcgccg tcacctgcgg
     6961 cgcggcggtg ctctttgagg cggcttttgt ctccgagcgc tcgcacaatg cgcggctctt
     7021 cgcgccgctg gcggaggcgc tcgctgtcat cggagaaagg cgcgcccgga tggtggtcgg
     7081 caccggaccg ggttcctaca ccggggtgcg catcgccatc gccgccgcgc agggtgtggc
     7141 ggtctcccgc gggtggacgg tggccggcct gccgacactc gcctgcgcgc cggggtgccg
     7201 cgtgcttggc gacgcgcggc gcggcttttt ttacatcgcc gcagtcagcg ccgcatcgga
     7261 ggttgggagt ctggaactcg ttgacgagcg gaccgcgcag gccgctgtga gcgccggcgg
     7321 agactgggtg actttcgacg cgcgcgcgcc gctcggactc gaccgcgtcc gcctcgcgca
     7381 gccggacgcc gtggcgctgg cgcggatcg
//
LOCUS       DAOMYJ010000243         7378 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Prosthecobacter sp. isolate AMR_MDS_5629
            NODE_3375_length_7378_cov_19.902226, whole genome shotgun sequence.
ACCESSION   DAOMYJ010000243 DAOMYJ010000000
VERSION     DAOMYJ010000243.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867850
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prosthecobacter sp. (wastewater metagenome)
  ORGANISM  Prosthecobacter sp.
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Verrucomicrobiaceae; Prosthecobacter.
REFERENCE   1  (bases 1 to 7378)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7378)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.30
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:34:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,742
            CDSs (total)                      :: 4,684
            Genes (coding)                    :: 4,676
            CDSs (with protein)               :: 4,676
            Genes (RNA)                       :: 58
            tRNAs                             :: 55
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7378
                     /organism="Prosthecobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3375_length_7378_cov_19.902226"
                     /isolate="AMR_MDS_5629"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1965333"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..391)
                     /locus_tag="PLP58_18845"
     CDS             complement(<1..391)
                     /locus_tag="PLP58_18845"
                     /inference="COORDINATES: protein motif:HMM:NF028007.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051219 - phosphoprotein binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HRJ10646.1"
                     /translation="MPYLAFNLNDGNEFVFDLLEERLSIGRDSKNDIVINNSYISGFH
                     AEFVRQPDGVYELVDLKSSNGTFVNGKRIERARVKGGDKLRFGQLDARFRERAPKGLA
                     PAEISARPPGPAKDAPARTDGRRGDTES"
     gene            473..1549
                     /locus_tag="PLP58_18850"
     CDS             473..1549
                     /locus_tag="PLP58_18850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009963215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HRJ10647.1"
                     /translation="MSLINYLKGQFLEIIQWQDDSRDTLSWRFPDEDKEIKRGAQLIV
                     RESQTAQFVYLGQFGDTFGPGKHDLVTDNIPILSTLKGWKYGFESPFKADIYFVNTRL
                     FTGNKWGTSNPVMMRDADFGIVRVRAFGTFDFRVCDVPLFLKEVAGSDHHFRLDEFAD
                     TMRSRIVSVFTEALATAKVPVLDLATRYGELGEALLPLINPQTQSKYGIEIISFVLEN
                     ASVPPEVEQAIDKRSSMSAIGNLNDYVKYQMAQGIAQGGGGMGGAAAEMAMGFGMAQQ
                     MMQQGAFNLQGQQQPPPLPGAATAVPTPAAPAGILTPAQVAQTLGVTEADVIASIEAG
                     DLKGKKIGAQYRVTQAALDEFLAH"
     gene            complement(1726..4278)
                     /locus_tag="PLP58_18855"
     CDS             complement(1726..4278)
                     /locus_tag="PLP58_18855"
                     /inference="COORDINATES: protein motif:HMM:NF016724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HRJ10648.1"
                     /translation="MPSEAQARITINKLLEAAGWCFTPDANGQVSVICEQRVTKKVYS
                     PHEDLGADFDKVPGGFVDYVLLNTDDKPVAVVEAKKESIDPLTAKEQARSYAESLGVA
                     HIFLSNGLVHYYWNLRQGNPVRVSRFLPLAELGKAAKWKPDAANLAAAVVDENYIAVS
                     QDPKWLGYSPEERVLAMVNQKIRLLRDYQVAAAQALQQAAVNKGWRFLFEMATGTGKT
                     LLSGAITKLFLRTNNATRVLFLVDRLELETQAWKHFNAYLGPDGIKTVIYKKQRDDWK
                     SADVVVTTIQSLATRNRYLTDFAPTDFQLLISDEAHRTISGNHRAIFEYFVGAKLGLT
                     ATPRDYLKGVNEQELGQEDPRALEKRILLDTYHTFGCGDGKPTFRFSLLDAVRHAPPY
                     LVNPVALDARTEITTAMLSKEGFAVKQAPDEDGNEAELIFTKRDYEKKFFSDETNLSF
                     VRCFLDNAWRDPITDEVGKTILFAVSRRHAAKLVKLLNEEAMRRWPEAYGAGSSFAVQ
                     VTSDIPGAQQMTIDFANNNLNGKSRWKRDDFRDYNTSRTRVCVTVGMMTTGYDCEDVL
                     NVALVRPIFSPTDFIQIKGRGTRLFTFKHEDGELEIEKPKAGFALFDFFANCEYFEKE
                     FNYDKKLNLPKTGEGEGEGGGGAGSEDDFTNTNPDPIKNVLRETIGEDGMRVDREMYR
                     ERFAAQAKAAAESHAVLRQALESEDWTGVEEFVKKTLLDKPDDFWNLDKLREIYRTDR
                     EPTLREIIQVVFGLSPGIATREQLAAEEFERFLATENVDSTKVRELRQIFMALLLDNR
                     VREDIEAGQFGKIMSVDMGVYQGLRLLGKTGIQQLMAYVDKHVRLSDFKKAA"
     gene            complement(4282..5106)
                     /locus_tag="PLP58_18860"
     CDS             complement(4282..5106)
                     /locus_tag="PLP58_18860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ10649.1"
                     /translation="MQRERVLITVKTYPTLSQSYVELVCTAGFRDDGSWVRIYPVPFR
                     LLDAEHQFEKLTWVDLPLVKRVKDTRPESFSPADRNDITLGAKMGTQDGWRERRELVL
                     RRGKVWTNLTDLIAEGKREEVSLATFKPARMIALECVKAETDWDAGKLATATKELQQM
                     DLLDDASVREDFKPANKLPYDFFYRFEDDQGREARLKILDWEIGVLYWNCLKAAHGDE
                     VTALVKVRQMYESNFFAKDLHLFLGTTYEWHKRAPNPWVIIGVFYPPPRPQLEFAL"
     gene            complement(5122..6000)
                     /locus_tag="PLP58_18865"
     CDS             complement(5122..6000)
                     /locus_tag="PLP58_18865"
                     /inference="COORDINATES: protein motif:HMM:NF016246.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 domain-containing protein"
                     /protein_id="HRJ10650.1"
                     /translation="MLFERQRLLLALLDTLGEPLKSTDFQKLLFLYTREWEQEPSYDF
                     VPYQFGGFSFTSYADKRKLITTGLLVDDANQWELTAEGRKAVAGERALRERLGRFRLR
                     HGGQRGDALIREVYLRHPYYATRSKIAPRVLKTAEERAKVEAARPVKKEPGVVTIGYE
                     GKTLERYLNQLLQDSVTLLCDVRRNPLSRKYGFSKSTLKNACENVGIRYEHLPQLGID
                     SEDRRNLDTQADYDALFEDYERKSLPKQTEALATIRQWVAEGERVALTCYEALPCQCH
                     RHCVAEALEGMTVKHL"
     gene            complement(6055..>7378)
                     /locus_tag="PLP58_18870"
     CDS             complement(6055..>7378)
                     /locus_tag="PLP58_18870"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF013579.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HRJ10651.1"
                     /translation="GLQPSAVIADDTPGALPQAGMGSAVGAAVVWRRVEKSTLLESRA
                     CSLQAETFFGEPNFEETTHAKIALGEFCRIGGSIKKDIDKTLPYIGSDSIESGTGRLT
                     QRESCEKQGIQGPVYEFAGPCLLYSKIRPYLNKLTITDFHGYCSSDIYPLKVDETKAL
                     PSFVMFNMLSSAFLDSISEFHERVSIPKINREQLFSASIPLPPLEEQRRIVAEIEGYQ
                     KVLDGARQILAGYKPSFDVDPEWETFPLAELIQEKPKNGYSGKPVAHPTQLKVLSLSA
                     TTSGKLDITKFKYLDEDIPLNAPCRCKRGDIYLQRGNTQELVGTAALFDVDATDFIYP
                     DLMIRVRANEERIMTSYLLHTLQSQPVREFLMSNATGAAGNMPKINQGIVESIPIPLP
                     PLEEQRQIVSELDAEAAQMEAVRSLIPRFEGKIQRVLDRVWGTTVQAV"
BASE COUNT         1350 a         2380 c         2119 g         1529 t
ORIGIN      
        1 cagactccgt gtcgcccctg cggccgtcgg tgcgcgcggg cgcgtctttg gcggggcctg
       61 gcggacgggc gctgatctcg gctggagcga gacccttggg cgcgcgctcg cggaagcggg
      121 cgtcgagctg gccgaagcgc agcttgtccc cgcccttgac gcgggcgcgc tcgatgcgct
      181 tgccgttgac gaaggtgccg ttcgaggact tcaggtccac cagttcataa acaccgtccg
      241 gctggcgcac aaactcggca tgaaaaccgc tgatgtagct gttgttgatg acgatgtcgt
      301 tcttcgaatc cctgccgatg gagagccgct cttcgagcag atcgaagacg aactcatttc
      361 cgtcgttgag gttgaaagcc agatagggca tgggcgcgcg ggcggcgaaa ttaggtttgg
      421 caaatcagta aatcaatcct aataaaccac gaatccccgc agcattcttc gcatgagcct
      481 catcaactac ctcaaaggcc agtttctcga aatcatccag tggcaggacg actcgcgcga
      541 caccctctcc tggcgctttc ccgacgagga caaggagatc aagcgcggcg cccagctcat
      601 cgtccgcgag tcccagaccg cgcagttcgt ctatctcggc cagttcggcg acaccttcgg
      661 ccccggcaag cacgacctcg tcactgacaa catccccatc ctctccaccc tcaagggctg
      721 gaaatacggc ttcgagtccc ccttcaaagc cgacatctac ttcgtcaaca cccgcctctt
      781 caccggcaac aaatggggca ccagcaaccc cgtgatgatg cgcgacgccg acttcggcat
      841 cgtccgcgtg cgtgccttcg gcaccttcga cttccgcgtc tgcgacgtgc cgcttttcct
      901 caaggaggtg gccggttccg accaccactt ccgcctcgac gagttcgccg acaccatgcg
      961 cagccgcatc gtcagcgtct tcaccgaggc gctcgccacc gccaaagtcc ccgtgctcga
     1021 cctcgccacc cgctacggcg agctcggcga ggccctgctg ccgctgatca atccgcagac
     1081 ccagtccaaa tacggcatcg agatcatcag cttcgtcctg gaaaacgcca gcgtcccccc
     1141 cgaggtcgag caggccatag acaagcgctc cagcatgagc gccatcggca atctcaacga
     1201 ctatgtgaag taccagatgg cccagggaat cgcccagggc ggcggcggca tgggcggcgc
     1261 cgccgccgag atggccatgg gcttcggcat ggcccagcaa atgatgcagc agggcgcctt
     1321 taatttgcag ggacagcagc agcctcctcc tctgcccggc gccgccaccg ccgttcccac
     1381 gccggcagcc cccgcgggca tcctcacgcc cgcccaggtc gcccagaccc tcggcgtcac
     1441 cgaggccgat gtcatcgcca gcatcgaagc cggcgacctc aaaggcaaaa agatcggcgc
     1501 ccaataccgc gtcacccagg ccgccctcga cgagttcctc gcgcattgac gccgcgtcat
     1561 tcacagacgg cggccctttg gcggtaaaaa tggcacgagg cgcggcgatc ccgcctcctg
     1621 caagagttgg gcgcgcgcgc tcgatgcgct ctcattccag cattgccttg attccaaagc
     1681 cgggacctca ttatcgtcct aaatgctgag cttcaacttt ctttgttaag ccgccttctt
     1741 gaaatccgac aacctgacgt gtttgtccac atatgccatc agttgctgaa tgcctgtttt
     1801 gccgagcagc ctcagcccct gataaacgcc catgtccacg gacatgatct tgccgaactg
     1861 tccggcttca atgtcctcac gcacccgatt atcgagcagc aacgccatga agatctggcg
     1921 cagttcgcgc acttttgtcg agtccacgtt ttcagtggca aggaaacgct cgaattcctc
     1981 tgccgcgagt tgttcgcggg tcgcgatgcc gggactgagg ccgaagacaa cctgaatgat
     2041 ctcacgcagg gtgggctcgc ggtcggtgcg gtagatctcg cgaagtttgt cgaggttcca
     2101 gaagtcgtcc ggcttgtcga gcagggtctt cttcacgaat tcctccaccc cggtccagtc
     2161 ctccgactcc agcgcctgac ggaggaccgc atggctttca gcggcggcct ttgcctgtgc
     2221 cgcgaagcgc tcgcggtaca tctcgcggtc aactcgcatc ccgtcctccc cgatggtctc
     2281 ccgaagcaca ttcttgatcg gatcgggatt cgtgttggtg aagtcgtctt cactgccagc
     2341 accaccgccg ccttctcctt caccttcgcc ggtcttcggc aggttcagct tcttgtcgta
     2401 gttgaactcc ttctcgaagt actcgcagtt ggcgaagaag tcgaagagcg cgaagcctgc
     2461 cttcggcttc tcgatttcaa gttcgccatc ctcatgcttg aaggtgaaca gacgtgtgcc
     2521 gcggcctttg atctggatga agtccgtcgg cgagaagatc ggtcgcacca gcgcgacgtt
     2581 cagcacgtcc tcgcaatcgt agccggtggt catcatgccg acggtcacgc agactcgggt
     2641 gcggctcgtg ttgtagtcgc ggaagtcatc ccgcttccac cgggacttgc cgttcaggtt
     2701 gttgttggcg aagtcgatgg tcatctgctg cgccccgggg atgtccgagg tcacctgcac
     2761 ggcgaaggat gagcctgcgc catacgcctc cggccagcgg cgcatggctt cctcgttgag
     2821 cagcttcacc agcttcgccg catggcggcg gctcacggca aagaggatcg tcttgcccac
     2881 ctcgtccgtg atgggatcac gccatgcgtt gtcgagaaag cagcggacga agctcaggtt
     2941 cgtctcgtca gagaagaact tcttctcgta gtcgcgcttc gtgaagatca actccgcttc
     3001 gttgccatcc tcgtcgggag cctgcttcac agcaaagccc tccttggaaa gcatcgcggt
     3061 ggtgatctcc gtgcgggcat ccagtgccac cgggttcacc agatagggcg gcgcatggcg
     3121 cacagcatcc agcaggctga aacggaaggt cggcttgcca tcgccgcagc caaaggtgtg
     3181 gtaggtgtcc agcaggatgc gcttctccag cgcacgcggg tcctcctgcc ccagttcctg
     3241 ctcgttcacg cctttcaggt agtcgcgtgg cgtggcagtg aggcccagct tggctcccac
     3301 gaagtattca aagatggccc ggtggttgcc gctgatcgtc cggtgcgctt catcgctgat
     3361 caggagctgg aaatccgtcg gcgcgaagtc cgtcaggtat cggttccgtg tggcgaggct
     3421 ttggatcgtg gtcaccacca catcggcgct cttccagtcg tcgcgctgct ttttataaat
     3481 gaccgtcttg atgccgtccg ggccgaggta ggcgttgaag tgcttccagg cctgcgtttc
     3541 cagttccagc cggtccacga ggaaaagcac gcgcgtcgca ttgttcgtgc ggagaaacag
     3601 cttggtgatc gcgccgctca gtagcgtctt gcccgtgccc gtggccatct caaagaggaa
     3661 acgccatccc ttgttcacgg cagcttgttg cagcgcctgc gccgccgcga cctgataatc
     3721 ccgcagcagg cggatcttct ggttcaccat cgccagcacg cgttcctcgg gcgagtagcc
     3781 cagccatttg ggatcttggg aaacggcgat gtagttctca tcgaccaccg ctgctgccaa
     3841 gttggccgcg tccggcttcc atttcgccgc cttgcccagc tccgccagcg gcagaaagcg
     3901 ggaaacgcgc accgggttcc cctgccgcag gttccagtag taatggacga gcccgttgct
     3961 caggaagatg tgcgccacgc ccaggctctc cgcgtagctg cgggcctgct ccttggcggt
     4021 cagcgggtca atgctttcct tcttggcctc caccaccgcc acgggtttgt cgtccgtgtt
     4081 cagcagcacg tagtccacaa agccgcccgg caccttgtca aagtccgccc ccaggtcctc
     4141 gtgcggcgaa tagaccttct tggtcacccg ctgctcgcag atcacgctca cctgtccgtt
     4201 cgcatccggg gtgaagcacc agcccgccgc ctccagcagt ttgttgatcg tgatgcgggc
     4261 ctgggcttcg gaaggcatga gttaaagggc aaattcgagc tgcggacgcg gtggcgggta
     4321 aaagacaccg atgatcaccc agggattcgg cgcacgtttg tgccattcat aagtcgtgcc
     4381 caggaaaagg tgaaggtctt tggcaaagaa attggactca tacatctgcc gcaccttcac
     4441 caaagcggtc acctcatctc catgggctgc tttcaggcag ttccagtaaa gcacgccgat
     4501 ctcccaatcg aggatcttca gccgagcttc acgtccttga tcgtcttcga accggtaaaa
     4561 gaagtcataa ggcagcttgt tcgccggttt gaagtcttcc cgcaccgagg cgtcatccag
     4621 caagtccatc tgctggagtt cctttgtcgc ggtcgccagc tttccggcat cccagtccgt
     4681 ctcggctttc acacactcca aagcaatcat ccgggccggc ttgaaggtcg ccagggaaac
     4741 ctcttcccgc tttccttccg cgatcaagtc ggtcaggttc gtccagacct tcccacgccg
     4801 gagcaccagt tcgcgccgct cgcgccagcc gtcctgcgtg cccatcttcg ctcccagcgt
     4861 gatgtcgttc cggtcagccg ggctgaagga ctccgggcgg gtgtccttta cgcgtttcac
     4921 caagggaagg tccacccagg tcaacttttc aaattgatgc tccgcatcca gcaggcggaa
     4981 cggcaccggg taaatgcgca cccacgagcc atcgtcacga aaacccgccg tgcagaccag
     5041 ttccacatag ctttgggaaa gcgtcggata cgttttgacc gtgatgagga cgcgctcgcg
     5101 ctgcatggcc gaggagaggg gtcaaaggtg tttcacagtc attccctcca gcgcctccgc
     5161 cacgcagtgc cggtggcatt ggcagggcag cgcctcgtag caggtcagcg ccacgcgttc
     5221 cccctccgcc acccactggc ggatcgtcgc cagcgcctcc gtctgcttcg gcaggctctt
     5281 gcgctcatag tcctcaaaca gcgcatcata gtctgcctgc gtgtccagat tccgccggtc
     5341 ctcggagtcg atgcccagtt gcggcaggtg ttcatagcgg atgcccacgt tctcacaggc
     5401 gttcttgagc gtgctcttgg aaaagccgta tttgcggctc agcgggttcc gccgcacatc
     5461 acagagcagc gtcacactgt cttgcaggag ctggttcagg tagcgctcca gcgtcttgcc
     5521 ctcgtagccg atcgttacca cgcccggctc tttcttgacc ggacgggccg cctccacctt
     5581 cgcccgttcc tccgccgtct tcagcacgcg gggggcgatc ttgctgcgcg tggcgtagta
     5641 ggggtggcgc agatacacct cccggatcag cgcgtccccg cgctgcccgc cgtgccgcag
     5701 gcggaagcgt cccagcctct cccgcagcgc gcgctccccg gccacggcct tccggccctc
     5761 ggcggtcagc tcccactggt tcgcatcgtc caccagcagg ccggtggtga tcagcttccg
     5821 cttgtccgcg taggaggtga aggagaaccc accgaactga tagggcacaa agtcatagct
     5881 cggctcctgc tcccattccc gggtgtagag gaagagcagt ttttggaaat cggtgctctt
     5941 gagcggctcc cccagcgtgt ccaggagggc cagcagcagg cgttggcgtt caaacagcat
     6001 ggcagggcaa gattacggcg ggggaacggt taagcaatcc aaacgactgc ctcctcagac
     6061 ggcttgcacc gtcgtccccc acacccggtc cagcacgcgt tggatcttcc cctcgaagcg
     6121 cgggatcaac gacctcaccg cctccatctg cgccgcctcc gcgtccagct cggacacgat
     6181 ctgccgttgt tcttccagcg gcggcagggg gatggggatg ctctccacga tgccttgatt
     6241 gatcttgggc atgttgcccg ccgctccagt ggcattgctc atgaggaatt cgcgaaccgg
     6301 ctgactttgg agggtgtgca gcaagtagga tgtcatgatc ctctcctcgt tggctcggac
     6361 ccgaatcatg agatcgggat agatgaagtc ggtggcatcg acgtcaaaca gggcggcagt
     6421 cccgaccagt tcctgggtgt tcccacgttg cagatagatg tcaccacgtt tgcagcggca
     6481 cggggcgttc aaagggatgt cctcgtccag atatttgaac ttcgtgatgt ccagcttgcc
     6541 cgatgtcgtg gcgctgaggc tcagaacctt gagttgggtg ggatgagcca caggcttgcc
     6601 agaatagcca ttcttgggct tctcctggat caattcggcc aacgggaaag tctcccattc
     6661 tggatcgaca tcgaaactcg gcttgtagcc cgcgagaatc tggcgggcac cgtcgagaac
     6721 cttctggtag ccctcgatct ccgccacgat ccgccgctgc tcctccagcg gcggcagcgg
     6781 gattgaggcc gagaataatt gttcacgatt gatctttgga atgctgacgc gctcgtggaa
     6841 ttccgaaatg gaatcaagaa aggcagatga gagcatgttg aacatcacaa agctcggcaa
     6901 tgctttggtc tcatcaacct tgagtgggta gatgtctgag ctgcaatagc cgtggaaatc
     6961 ggtgatcgtc agcttgttca gataaggtcg aatcttgctg taaagcaggc aaggcccagc
     7021 gaactcataa actggacctt ggataccttg cttctcgcat gattctcgtt gggtgagcct
     7081 gcctgtacct gattcgatgc tgtcagagcc gatatatggc agagtcttgt cgatgtcttt
     7141 tttgatactt ccgccgatac ggcagaactc gccaagggcg atctttgcat gtgtggtttc
     7201 ttcaaagttg ggttcaccaa agaacgtctc cgcctgaagg ctgcatgccc ggctctccag
     7261 cagcgtggat ttctccaccc gcctccaaac cacggcagcg ccaacggcgc tacccatccc
     7321 agcctggggc aacgccccag gtgtgtcatc cgcgatcacg gcggagggct ggaggccc
//
LOCUS       DAOMYJ010000244         7331 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Prosthecobacter sp. isolate AMR_MDS_5629
            NODE_3396_length_7331_cov_16.862150, whole genome shotgun sequence.
ACCESSION   DAOMYJ010000244 DAOMYJ010000000
VERSION     DAOMYJ010000244.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867850
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prosthecobacter sp. (wastewater metagenome)
  ORGANISM  Prosthecobacter sp.
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Verrucomicrobiaceae; Prosthecobacter.
REFERENCE   1  (bases 1 to 7331)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7331)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.30
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:34:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,742
            CDSs (total)                      :: 4,684
            Genes (coding)                    :: 4,676
            CDSs (with protein)               :: 4,676
            Genes (RNA)                       :: 58
            tRNAs                             :: 55
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7331
                     /organism="Prosthecobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3396_length_7331_cov_16.862150"
                     /isolate="AMR_MDS_5629"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1965333"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..300)
                     /locus_tag="PLP58_18875"
     CDS             complement(<1..300)
                     /locus_tag="PLP58_18875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ10652.1"
                     /translation="MRHQLYFPPRQADQVAWLRQFRHALPGCMERLEAVRPLEPVLRD
                     EVPRDLDWLIFIMGPAMTRTRVLGMAMTSVQRALLMGDARGGKPVSLPALSLPEPP"
     gene            complement(1087..1239)
                     /locus_tag="PLP58_18880"
     CDS             complement(1087..1239)
                     /locus_tag="PLP58_18880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ10653.1"
                     /translation="MFSPPLFPRLIRLLPPACIAALLLASGVRNEAAPWQRELWNESL
                     RVAGGV"
     gene            complement(1354..2493)
                     /locus_tag="PLP58_18885"
     CDS             complement(1354..2493)
                     /locus_tag="PLP58_18885"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006635 - fatty acid beta-oxidation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiolase family protein"
                     /protein_id="HRJ10654.1"
                     /translation="MTPVHIIEAKRSPIGRFGGGLKDIPAADLAFAVADAVAPWELRA
                     HLDQVILGQVLQAGSGMNVARQCALKLGALEHVPACTVNMACGSSLKAAALAADAIRS
                     GECRLVLAGGVESMSRAPHYVSDARWGKKLGPIEMADAIFTDGLTDPLLKIGMGETAE
                     RIADKHGITRLEQDLFAARSQLRADMHRASWAREIIPVGEVPADEHPRADTTVESLGK
                     LKPAFRKDGTVTAGNASGINDGAAMVLLASDDALQTHGLTSRARVIATAAVGCDPALM
                     GLGPVGAIRKVCALAGWRLDEVDAIEINEAFAVQTLGCARELDLDQEKLNPRGGAIAL
                     GHPIGASGARVLVTLLHWMEDQDLRRGIASLCIGGGMGIAMAVER"
     gene            complement(2490..3947)
                     /locus_tag="PLP58_18890"
     CDS             complement(2490..3947)
                     /locus_tag="PLP58_18890"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF013323.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-transferase"
                     /protein_id="HRJ10655.1"
                     /translation="MRAPATYTTASKAVSAIPDGATVAVGGFVGAGHPEMLTAALEQR
                     FLETGRPRGLTLLYCAGQGDRAARGLNHLAHPGLLKRVIGGHWNLAPKLGELALNNEI
                     EAYNLPQGVLCALMRETAARRPGLITQVGLGTFMEPEQGGGRLNERTTEPLVERVTLD
                     GRDWLRYKPVPVHAGLIRATYADTLGNLDMESEALIGEVLPIAQAAKNHGGIVIAQIE
                     GMLDRLPDPKSVRVPGILVDYIVASGGINHDQTFAEEFNEEFVVSGGAFSLPPMPESD
                     RKRIATRALREIRPGEIVNLGIGLPEGVAAVAAETGRLGEFTLTVESGPIGGVPASGL
                     NFGCSHFPEAVIDQPAQFDFYDGGGLDIAVLGAVEVDAQGSVNVASMGGRFAGVGGFM
                     NIAHSARRLVFCSTFLAKEQPKFVEKIRHVCFHGPSALARGQQVLYVTERAVFELTPQ
                     GLRLIETAPGVDVGNDILARMQFRPAIAENLATLS"
     gene            complement(3964..6735)
                     /gene="glnD"
                     /locus_tag="PLP58_18895"
     CDS             complement(3964..6735)
                     /gene="glnD"
                     /locus_tag="PLP58_18895"
                     /EC_number="2.7.7.59"
                     /inference="COORDINATES: protein motif:HMM:TIGR01693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008773 - [protein-PII]
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0018177 - protein uridylylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="[protein-PII] uridylyltransferase"
                     /protein_id="HRJ10656.1"
                     /translation="MTEREYKRRIEAAAQKVFAGAGPAPRGKAVTLKLCRQFLRLKEQ
                     RIKQRHRAGWSGVEICWMRSEMLDYLVRLLWEECAAAQPGGAALKISVVAHGGYGRRA
                     MCPGSDVDLTFLVPGNGAQVPPEVTKLVVSFQTALWDLKLKVGQGGTRSVGDCLRHAN
                     EERQAKTALIEARFLTGDRAAFDDFRARFDAECIRGREKEFLRHRQEDLAARHEKHGG
                     TPFVQEPHVKNGCGGLRDYQNLIWMTYAKLGTLDPRDLVARGLISAAGWREVERAYDL
                     ILRARNEMHYSEKRGEDVLTLRLQGVVAGHLGYKHKRVLRRIECFMKDYYMATRDVLQ
                     RSSEIMDGFHLEQLEGARPRKSLLGFLARRKDARRVEKFDGFHSKNGRVFPDGEDIFK
                     EDPQRLMRLFSHTQQRHLRLSPELFQLVQKSFRLVNAGFRYNREIREVFEAILSRKGE
                     VARTLRQMHRVGFLGRYIPEFGALTCLVQHEFFHRFTADEHTLRTLDKLDELAGPPQK
                     GGMAFYQQIFQELQQPAMLYLALVMHDTGRAANKKSHDDESTLLADAVARRMVIKGER
                     RKLLLFLVDNHLLMYRVATTSNLEDPKVIEDFVQIVRTKENLDALLVMTQADSRGTSE
                     ASWTGYKETSIRQLYHSALEFMKAPADFMRRVSVPIEDVRAEVAGLLPAGFEEETSAH
                     FEHMPRSYFNFRDPAQIAAHIRQFRDFFVQLSGEDARAGLLPVMVWNDFPAQGYSELV
                     VTCWDRHLLLARVSGALAAEGINILSADLYQRSDHLVLDIFRVCTTGMTAVTNRSAKQ
                     RVESAVRAAFLSQSFDFAEAIAKSRRGIPGYDEVMAEIPQRVHINNDLSPDQTVVELQ
                     IVDRLGLLHDIFMAIGRLGLSVTHARIATEKGVALDSVYVQDENGRKLRDRRALDRLR
                     ARIEDAVFG"
     gene            complement(6745..7278)
                     /locus_tag="PLP58_18900"
     CDS             complement(6745..7278)
                     /locus_tag="PLP58_18900"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018970762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003999 - adenine
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HRJ10657.1"
                     /translation="MSPDTLERLRSTIRDVPDFPQPGILFKDITPVLADAVLLRQAIE
                     GMAAAFADQKVDKVVGIDARGFIFGALMAARLGAGFVPVRKKGKLPWRTRGLDYTLEY
                     GSNSVEMHEDALAPGEKVVLADDLLATGGTAGAAVRLIQESGADLLGSAFFIELGFLD
                     GRSKLAGSGPVHALLTF"
BASE COUNT         1145 a         2479 c         2544 g         1163 t
ORIGIN      
        1 gggcggctcc ggcaggctca gcgccggcag ggacaccggc ttcccgccgc gcgcatcccc
       61 catgagcagc gcccgctgca cggacgtcat cgccatgccc agcacgcgcg tccgggtcat
      121 cgccggtccc atgatgaaaa tcagccagtc caggtcccgc ggcacctcgt cccgcagcac
      181 cggctccagc ggccgcaccg cctccagacg ttccatgcag cctggcagcg cgtggcggaa
      241 ctgccgcagc cacgccacct ggtccgcctg gcggggaggg aaataaagct ggtgtctcat
      301 ggcatgaaaa aaaattcccg accccggccg caaaggccgc ccgaaaggag cgcggacact
      361 cccgcccgcc gcgggcgaag cgagccgcaa cgctgcgggc aagagtgccc acactccgat
      421 tccaagccgc gcgcggggaa ggtccgggcg cagtcagagc cgtgcggagg attatccttt
      481 ttggctcagg atgtcaattc gtgaagtggg gcatgatcct tattcaaaaa ggatacatgc
      541 tcggcgatcc aggcacagcc ccgagtcggc agggaaacat ccttttccag aaaggatatt
      601 cccccgccaa gcggggtcaa cccctgaggc atccgggcag catcctattt caaaaaggat
      661 aatgccccgc acattcgagc aagtccccgg ctcgaaagtt cggcatcctt tcccgaaaag
      721 gataaacccc cagtccccga agctcacgcc cagacggccc accatgcatc ctttttaaaa
      781 aaggattcat cacaaatccg ccgaacaaga ccccgagcca cccaaccgcg tgcccggagc
      841 ctttccgacc tgtgcccgaa tcgtgcccgt cctgcccgga gtcacgcccc gagcggctgg
      901 agcctccggc ggtgcgcctg gagccgtgtg ccgcgagttt gggaacccac acccccacgc
      961 gccaatccgc atgagttcag acccggtgcg caggaacgca gccccagtcc gccgcgcgtc
     1021 ctttctgcgg atcggccgcg ctattcccct gcgccgggca ttcgccgagc ttggggcgca
     1081 gcgccctcac accccgcccg ccacgcgcag ggattcattc cacagctcgc gctgccaggg
     1141 ggcggcctcg ttgcggacgc cggacgcgag gagcagggcg gcgatgcagg ccgggggcag
     1201 caggcgaatg aggcggggga aaagcggggg gctgaacatg ccgcgagtgt ggcgcggctg
     1261 gcgcgggcgt gctttgggcc gattgtcaga agttgacggc gggatgaaat tttcctcgcc
     1321 aaaacgcggc ggagagcacc cgagcacgcc gcctcaccgc tccaccgcca tggcgatgcc
     1381 catgccgccg ccgatgcaga ggctggcgat gccgcgccgc agatcctggt cctccatcca
     1441 gtgcagcagg gtgacgagca cgcgcgcgcc gctggcgccg atggggtggc cgagggcgat
     1501 ggcgccgccg cgcgggttga gcttttcctg atccagatcc agctcgcgcg cgcagccgag
     1561 ggtctgcacg gcgaaggctt cgttgatctc gatggcgtcc acctcgtcga gccgccaccc
     1621 ggccagggcg cacaccttgc ggatggcgcc gacggggccg aggcccatca aggcggggtc
     1681 gcaacccacg gcggcagtgg cgatgacgcg ggcgcgggag gtgaggccgt gggtttgcag
     1741 cgcgtcgtca ctggcgagca gcaccatggc ggcgccgtcg ttgatgccgg aggcgttgcc
     1801 ggcggtgacg gtgccgtctt tgcgaaaggc gggcttcaat ttgccgaggg actccacggt
     1861 ggtgtcggcg cgcgggtgct cgtcggcggg gacttcgccg acggggatga tctcgcgcgc
     1921 ccaggaggcg cggtgcatgt ccgcccggag ctggctgcgc gcggcgaaga ggtcctgctc
     1981 caggcgggtg atgccgtgct tgtcagcgat gcgttcggct gtctcgccca tgccgatttt
     2041 caggagaggg tcggtgaggc cgtcggtgaa aatggcgtcc gccatctcga tggggccgag
     2101 ctttttcccc cagcgcgcgt cgctcacata atgcggcgcg cggctcatgc tctcgactcc
     2161 gccggcgagc acgagccggc attcgccgga gcggatggcg tcggcggcga gggcggcggc
     2221 tttgagggag gagccgcagg ccatgttcac ggtgcaggcg ggcacatgct ccagcgcgcc
     2281 gagcttcagg gcgcactggc gcgccacatt catgccgctg ccggcctgga gcacctggcc
     2341 gaggatgact tgatccagat gcgcgcgcag ttcccagggc gccacggcat ccgccacggc
     2401 aaaggcgaga tcggcggcgg ggatgtcctt caatccgccg ccgaacctgc cgatggggga
     2461 gcgctttgcc tcgatgatgt ggacgggtgt catgacaggg tggcgagatt ttcggcaatg
     2521 gccgggcgga actgcatccg cgcgaggatg tcgtttccca catccacgcc gggggcggtc
     2581 tcgatgaggc gcaggccctg cggggtgagt tcgaagacgg cgcgctcggt gacgtagagc
     2641 acctgctggc cgcgcgcgag ggcggagggg ccgtggaagc agacgtggcg gattttttcg
     2701 acaaacttgg gctgctcctt cgccaggaag gtgctgcaaa agaccagccg ccgcgcgctg
     2761 tgggcgatgt tcatgaagcc gcccaccccg gcgaagcgcc cgcccatgct ggccacgttc
     2821 acgctgccct gcgcgtccac ctccaccgcg ccgagcacgg cgatgtccag cccgccgccg
     2881 tcgtagaaat caaactgcgc gggctggtca atgacggcct cggggaagtg gctgcagccg
     2941 aagttcagcc cggaggcggg cacgccgccg atggggccgc tctcgacggt gagggtgaac
     3001 tcccccagcc gcccggtctc cgcggcgacg gcggccacgc cctccggcag gccgatgccg
     3061 agattgacga tctcccccgg gcgtatctcc cgcagcgcgc gtgtggcgat gcgcttgcgg
     3121 tcggactccg gcatgggagg cagggaaaac gcgccgccgc tgacgacaaa ctcctcgttg
     3181 aactcctcgg cgaaggtctg gtcatggttg attccgccgg aggcgacgat gtaatccacc
     3241 aggatgccag ggacgcgcac cgactttgga tcgggcaggc ggtcgagcat gccctcgatc
     3301 tgggcgatga cgatgccgcc gtggtttttc gccgcctgcg cgattggcag cacctcgccg
     3361 atgagcgcct cactttccat gtcgagattc cccagcgtgt ccgcgtaggt ggcgcggatg
     3421 aggcccgcat ggacgggcac cggcttgtag cgcagccagt cgcgcccgtc caaagtcacg
     3481 cgctccacga gcggctcggt ggtgcgctcg ttgagcctgc cgccgccctg ctccggctcc
     3541 atgaaggtgc cgaggcccac ctgggtgatg aggccgggcc gcctggcggc ggtctcgcgc
     3601 atcagggcgc agagcacgcc ctggggcagg ttgtaggcct cgatctcgtt gttgagcgcc
     3661 agctcgccga gcttcggggc caggttccaa tgcccgccga tgaccctttt caacaggccg
     3721 ggatgcgcca ggtggttgag gccgcgcgcg gcgcggtcgc cctggccggc gcagtagagg
     3781 agcgtcaggc ccctgggccg ccccgtctcc agaaagcgct gctccagcgc ggcggtgagc
     3841 atctcggggt gcccggcgcc gacgaagccg cccaccgcca ccgtggcgcc atccgggatg
     3901 gcggagacgg ccttcgaggc ggtggtgtag gtggcgggcg cgcgcatgtc ggagccgggt
     3961 gggtcagcca aagacggcat cctcgatcct ggcgcgcagg cggtcgagcg ccctgcggtc
     4021 ccggagcttg cggccgtttt cgtcctgcac atagacggag tccagcgcca cgcccttttc
     4081 ggtggcgatg cgcgcgtggg tgacgctgag gccgagccgg ccgatggcca tgaaaatgtc
     4141 atgcagcagg ccgaggcggt ccacgatctg cagctcgacc acggtttgat ccggggagag
     4201 gtcgttgttg atgtggacgc gctgcgggat ctcggccatc acctcgtcat agccggggat
     4261 gccgcggcgg gatttggcga tggcttcggc gaagtcgaag ctctggctga ggaaggcggc
     4321 gcgcacggcg ctctccacgc gctgtttggc gctgcggttg gtcacggcgg tcatgcccgt
     4381 ggtgcagacg cggaagatgt ccagcacgag gtggtcggag cgctggtaaa ggtcggcgct
     4441 caggatgttg atgccctcgg cggcgagcgc gccggagacg cgcgcgagca gcagatggcg
     4501 gtcccagcag gtgacgacga gctcgctgta accctgggcg ggaaaatcgt tccagaccat
     4561 gacgggcagc aggccggcgc gggcgtcctc cccggagagc tggacaaaaa aatcgcggaa
     4621 ctggcggatg tgcgcggcga tctgggcggg gtcgcggaag ttgaagtagc tgcgcggcat
     4681 gtgctcgaag tgcgcggagg tctcctcctc gaaaccggcg ggcagcagcc ccgccacctc
     4741 cgcgcgcacg tcctcgatgg gcacgctgac gcggcgcatg aagtcggcgg gcgccttcat
     4801 gaactccagc gcgctgtggt agagctggcg gatggaggtc tccttgtagc cggtccagct
     4861 cgcctcgctg gtgccgcgcg agtcggcctg ggtcatgacc agcagcgcgt cgaggttctc
     4921 cttggtgcgc acgatctgga cgaagtcctc gatgaccttc gggtcctcca ggttcgatgt
     4981 ggtggccacg cggtacatga gcaggtggtt gtccaccagg aagagcagca gcttgcgccg
     5041 ctcccccttg atgaccatgc ggcgggccac cgcgtccgcc agcagggtgc tctcgtcatc
     5101 gtgggatttt ttgttggcgg cgcgcccggt gtcatgcatg acgagcgcca ggtagagcat
     5161 ggcgggctgc tgcagctcct ggaagatctg ctggtagaaa gccatgccgc ccttctgcgg
     5221 cgggccggcc agctcgtcga gcttgtccag cgtgcgcagg gtgtgctcgt ccgcggtgaa
     5281 gcggtggaag aactcgtgct gcaccaggca ggtcagcgcg ccgaactccg ggatgtagcg
     5341 gcccaggaag cccacgcggt gcatctggcg cagcgtgcgc gccacctcgc ccttgcggga
     5401 gaggatggcc tcgaaaacct cgcggatttc cctgttgtaa cggaagccgg cgttgaccag
     5461 gcggaagctc ttctgcacga gctggaagag ctccgggctg aggcgcagat gccgctgctg
     5521 ggtgtgggaa aagaggcgca tgagacgctg cgggtcctcc ttgaaaatgt cctccccgtc
     5581 ggggaagacg cggccgtttt tgctgtgaaa gccgtcgaac ttctccaccc gccgcgcgtc
     5641 cttcctccgc gccaggaaac cgagcaggct cttgcgcggc ctggcgccct cgagctgctc
     5701 gaggtggaag ccgtccatga tctcgctgct gcgctggagc acgtcccgcg tggccatgta
     5761 gtagtccttc atgaagcact cgatgcggcg cagcacgcgc ttgtgcttgt agcccagatg
     5821 gccggccacc acgccctgga ggcgcagggt cagcacatcc tccccgcgct tttcgctgta
     5881 gtgcatctcg ttgcgcgcgc gcaggatgag gtcataggcc cgctccacct cgcgccagcc
     5941 ggcggcggag atgaggccgc gcgccaccag gtccctcggg tccagcgttc cgagcttggc
     6001 ataggtcatc cagatcaggt tctggtagtc gcgcaggccg ccgcagccgt ttttcacatg
     6061 cggctcctgg acgaaggggg tgccgccgtg tttttcgtgg cgcgcggcca ggtcctcctg
     6121 ccggtggcgc aggaactcct tttcccgccc ccggatgcac tccgcgtcga agcgcgcgcg
     6181 gaaatcatca aaagccgccc tgtccccggt gaggaagcgc gcctcgatga gcgccgtctt
     6241 cgcctgccgc tcctcgttcg cgtggcgcag gcagtccccc acgctgcggg tgccgccctg
     6301 gcccaccttc agcttcaggt cccacagcgc ggtttggaag gagaccacca gcttcgtcac
     6361 ctccggcggg acctgcgcgc cgttgccggg caccagaaag gtgaggtcca catcactgcc
     6421 cgggcacatg gcccgccgcc cgtagccgcc gtgcgccacc acgctgatct tgagggccgc
     6481 cccgcccggc tgcgccgccg cgcactcctc ccacagcagg cgcaccaggt agtcgagcat
     6541 ctccgagcgc atccagcaga tctccacccc gctccagccg gcgcggtggc gctgcttgat
     6601 gcgctgctcc ttgagccgga ggaactgcct gcacagcttc agcgtcaccg ccttcccgcg
     6661 cggcgcgggg ccggcgccgg cgaagacctt ctgggccgcc gcctcgatgc ggcgcttgta
     6721 ctcgcgctcc gtcatgcggg gaaatcaaaa ggtaagcagc gcgtgcaccg gcccgctgcc
     6781 cgccagcttc gagcgcccgt ccaggaagcc cagctcgatg aaaaacgccg accccagcag
     6841 gtccgcgccc gactcctgga tgaggcgcac cgccgcgccc gccgtgccgc ccgtggccag
     6901 cagatcgtcc gccagcacca ccttctcccc cggcgccagc gcgtcctcgt gcatctccac
     6961 gctgttgctg ccgtactcca gcgtgtaatc cagcccgcgc gtcctccagg gcagcttgcc
     7021 ctttttgcgc accggcacga agcccgcgcc cagccgcgcc gccatcagcg cgccgaagat
     7081 gaagccgcgc gcgtcaatgc ccaccacctt gtccaccttt tgatccgcga aggccgcggc
     7141 catgccctcg atggcctggc gcagcagcac cgcgtccgcc agcaccggcg tgatgtcttt
     7201 gaaaaggatg cccggctggg ggaagtcggg cacatcacgg atcgtggagc ggaggcgttc
     7261 gagggtgtcg gggctcatgg ccgcgcagtg tgcgggagcg cgcggcggtt gcaagcggca
     7321 atgcccgcgc t
//
LOCUS       DAOMYJ010000245         7316 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Prosthecobacter sp. isolate AMR_MDS_5629
            NODE_3407_length_7316_cov_25.992288, whole genome shotgun sequence.
ACCESSION   DAOMYJ010000245 DAOMYJ010000000
VERSION     DAOMYJ010000245.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867850
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prosthecobacter sp. (wastewater metagenome)
  ORGANISM  Prosthecobacter sp.
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Verrucomicrobiaceae; Prosthecobacter.
REFERENCE   1  (bases 1 to 7316)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7316)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.30
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:34:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,742
            CDSs (total)                      :: 4,684
            Genes (coding)                    :: 4,676
            CDSs (with protein)               :: 4,676
            Genes (RNA)                       :: 58
            tRNAs                             :: 55
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7316
                     /organism="Prosthecobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3407_length_7316_cov_25.992288"
                     /isolate="AMR_MDS_5629"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1965333"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(117..287)
                     /locus_tag="PLP58_18905"
     CDS             complement(117..287)
                     /locus_tag="PLP58_18905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ10658.1"
                     /translation="MKNDLTHEKEEFISACLEWALKQEPTQQILEWVEWAMDRLGIPA
                     ARARGKREGGGL"
     gene            complement(277..519)
                     /locus_tag="PLP58_18910"
     CDS             complement(277..519)
                     /locus_tag="PLP58_18910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ10659.1"
                     /translation="MSNRLFSQREGFALVCPVIRAAPSRKRRRERRLLLTAVVLMLVL
                     PLAVCLTWHVGMRLVEARASDGGGNDNNEIFHENEK"
     gene            complement(516..731)
                     /locus_tag="PLP58_18915"
     CDS             complement(516..731)
                     /locus_tag="PLP58_18915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ10660.1"
                     /translation="MIRTTASRLRKGQRVKLRTSAQMWKCLESCAESKDGYACVAIGY
                     HKQWLSPGYKVQVPLEDDPVLGGAALP"
     gene            complement(1003..1203)
                     /locus_tag="PLP58_18920"
     CDS             complement(1003..1203)
                     /locus_tag="PLP58_18920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ10661.1"
                     /translation="MKEPTLKEMRELVKFRRRVKELLEETPSYDAETILSARQVGQQL
                     DELRRGLQAALDASAPVKGGAQ"
     gene            complement(1266..1460)
                     /locus_tag="PLP58_18925"
     CDS             complement(1266..1460)
                     /locus_tag="PLP58_18925"
                     /inference="COORDINATES: protein motif:HMM:NF024064.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribbon-helix-helix domain-containing protein"
                     /protein_id="HRJ10662.1"
                     /translation="MKKSATRITVTLPPELDEAASKVSLETGVSISDLMRQGMIRIIT
                     ERLNTGNVTLLQLPPAQQAA"
     gene            1543..1821
                     /locus_tag="PLP58_18930"
     CDS             1543..1821
                     /locus_tag="PLP58_18930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ10663.1"
                     /translation="MAKKDARILGIRLDEKDATRLQTIEDTLCITGVSLARAALKAAL
                     DHWETHGTITLPLEIRPKSAAFLLNEEPKNYPPRAHDPGAQTPKQRAG"
     gene            2056..2361
                     /locus_tag="PLP58_18935"
     CDS             2056..2361
                     /locus_tag="PLP58_18935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ10664.1"
                     /translation="MNPDTPLTYKMPRRVKSGGSGCAALVFFLLALACGAVGLFAFWP
                     ALLPAGILLGLAILSDSKTRIEHHCGHCGNPVASTSRSCPHCRADLLTLPWHRRIWH"
     gene            2364..2552
                     /locus_tag="PLP58_18940"
     CDS             2364..2552
                     /locus_tag="PLP58_18940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ10665.1"
                     /translation="MHAIHIPLAAALGLIAMATLVPAQKNVGMCQPYAETCPHCTDCT
                     QCGHCAKKGGRCSVCWPR"
     gene            complement(2549..2749)
                     /locus_tag="PLP58_18945"
     CDS             complement(2549..2749)
                     /locus_tag="PLP58_18945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ10666.1"
                     /translation="MSTNEDTASGRGAPAGNQNAAKDDGYDTVLRIRCHAADKGGWKL
                     KAERAGLDLSEWIRTRLNRARD"
     gene            complement(2746..3387)
                     /locus_tag="PLP58_18950"
     CDS             complement(2746..3387)
                     /locus_tag="PLP58_18950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012419831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA];
                     GO_process: GO:0043412 - macromolecule modification
                     [Evidence IEA];
                     GO_process: GO:0044238 - primary metabolic process
                     [Evidence IEA];
                     GO_process: GO:0044260 - cellular macromolecule metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HRJ10667.1"
                     /translation="MSDLFDQQPEIERLRRALCAAIAELPQREQIHQINQTKIALHEI
                     SPFKAEPVDCVLWFPCDDITANDYNPNRVAPPEMKLLERSIEADGYTQPIVAMQEADK
                     PVKTTVDGFHRGRIGKESKKIRERVHGHLPVVLINKDLSNRMAATIRHNRARGTHDIG
                     LMSGIVAELVDCGMGDAWIMKNIGMDADELLRLKQITGLAALFKDQQFSKAWE"
     gene            complement(3384..3770)
                     /locus_tag="PLP58_18955"
     CDS             complement(3384..3770)
                     /locus_tag="PLP58_18955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ10668.1"
                     /translation="MKTIQLTNTDDEFYPRMGPFLANRTVIKAIGYPLYDDDGKVWHL
                     ALDDGQVVGFCYAWQRPSKLDVGSFYAMHEQAAAALAKAVIAHMQPGTAVIVTKSETV
                     VRGAKKAGFKGHLPKGKFTQLEHTKS"
     gene            complement(3773..5068)
                     /locus_tag="PLP58_18960"
     CDS             complement(3773..5068)
                     /locus_tag="PLP58_18960"
                     /inference="COORDINATES: protein motif:HMM:NF023349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3440 domain-containing protein"
                     /protein_id="HRJ10669.1"
                     /translation="MTGKNVLQAAQERLALVFAEFEYVYVSFSGGKDSSVILHLALDY
                     VRQHCPNRRIGVFHIDYEAQYQATTDHVRETFDALPEWCDKYWCAMPLTVPCCTSMSQ
                     STWVPWNPAERDIWVRDLPEGSIHLGNHSFDFYRFGMEDYDFQKQFGDWVARTTGKKT
                     ACLIGVRAEESYDRRIMLANTVNARKYPGILWSTGGIDDSGRQFNFYPIHDWSVDDVW
                     IYHGRFGKPYNRLYDLFHQAGLTLPQMRVASPFISQGIESLKLYRAVEPHTWGKLVSR
                     VNGVNFSGIYGGTTAMGWKSITKPKHHTWKSYLEFLLATLPAETRQRYEEKFRVSKEF
                     WTTRGGVLDDDVVEDIRRTGEKVEIKKESSYKTVKQTVVFQDYPDELDITEFKAVPSY
                     KRMVVCILKNDHLCKYMGFTLTKEEQQRRAAVLAKYKDL"
     gene            complement(5077..5484)
                     /locus_tag="PLP58_18965"
     CDS             complement(5077..5484)
                     /locus_tag="PLP58_18965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ10670.1"
                     /translation="MTINHITLMTGDNVLHRLDIIPPEVVEQCRELLPEGGQIPGFPA
                     FRVEIHAPVFTIWRGREPIATCGLGQGEDDVWSTLRDLQARFAPVKARPPAGRWLAVV
                     LLPGLLTTAREDVHWLADFERCLAAAMLLEDVA"
     gene            complement(5481..6932)
                     /locus_tag="PLP58_18970"
     CDS             complement(5481..6932)
                     /locus_tag="PLP58_18970"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF012403.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HRJ10671.1"
                     /translation="MKWNLQPGRWTMICAMTDAPVTPLMQHQAAAVAKLHRSRVGALF
                     MEMGTGKSRTAIEIAAQRRDRISRVLWFCPVSVKETIRREIEKHWPGQTVHVFDHKTC
                     EGKMPEARWYVIGLESMSASVRVIVAAESLIDANAMVIVDESSYIKGHRARRTTYVTK
                     IAARARYRLILTGTPISQGVVDLFAQMRFLSTKILGYKSFHSFAANHLEYHEKFPGMI
                     VRAHNTDWLAAKMAPYVYQVTKEECLNLPDKLYTTRYCTLTPEQREAYERAKDEMLQE
                     YLDNEGEVTAATIFRLFNALQCIVSGWWNRKSEKDEEAELLEFPHNRLELLDSVLGEI
                     PEGEKVIIWTKHRRSLAQIEASLGASVAVFHGGLSESERTRQLDSFRSSRRYLVATPD
                     CGGHGLNLTEASYAVYYDNQFKFANRLQSEDRCHRIGQARKVTYIDLHCSGTIDDRIQ
                     NCLAKKGDVVNEFRAEIQRMRDKKTIIQAIKAL"
     gene            complement(6880..>7316)
                     /locus_tag="PLP58_18975"
     CDS             complement(6880..>7316)
                     /locus_tag="PLP58_18975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ10672.1"
                     /translation="RLLKAGFRVRLAPEEVRMRVAAGQFRPHTTRRVFWQPDQKKFRV
                     QWDRESGDDFYEQAKRLPGAKWSKELRSMLVPLEAWESVEDFAERQGFRLSPGAEKAV
                     AAGREADARSLKVQLGAEPEMEKPVNEMEPATGEVDDDLRDD"
BASE COUNT         1340 a         2520 c         1968 g         1488 t
ORIGIN      
        1 cacctcgtcg agcagcttca gggtgcggtc atgcagccgc gccgccgccc agcgccccac
       61 ccacagcccc gcgaagcccg aggccaggca caccgccaac gccacatgca aggggatcac
      121 agcccacccc cttccctctt tccgcgcgcg cgggctgccg ggatgccgag ccggtccatc
      181 gcccactcga cccactccag gatctgctgc gtcggctcct gcttcagcgc ccactccagg
      241 cacgcgctga tgaattcctc cttctcatgt gtcagatcat ttttcatttt catggaaaat
      301 ttcgttgttg tcgttaccgc caccgtcaga tgcccgtgcc tcaaccagcc gcatccccac
      361 atgccaggtc aggcacaccg ccagcggcag caccagcatc agcaccaccg ccgtgagcag
      421 cagcctccgc tcccgccgcc gtttgcgaga cggcgccgcc cggatcaccg gacacaccag
      481 cgcaaagccc tcgcgctgag aaaaaagccg gttgctcatg gcagtgccgc ccctcccagc
      541 accggatcat cctcgagcgg cacctgcacc ttgtagccag ggctcagcca ttgcttgtgg
      601 tagccgatgg ccacgcatgc gtagccgtcc ttgctctccg cgcagctttc caggcacttc
      661 cacatctgcg ccgaggtgcg cagcttcaca cgctgcccct tgcgcagtcg ggaggccgtc
      721 gtgcggatca tgagcgctct cactgcgcac ccccttccat catccgccgc cactgccgcg
      781 cctgcgccgg ggtccaccag gccaaaaaat ccccgctggc ccgctccgtc accaaaaatt
      841 ccgcccgcgc cgtgcgccgc ttgccgcgat acaccggcca gcccgccgcc gactccacgc
      901 gcacctccag acggcacgcc gccacctccg cccggcacca ggccgggcag ccacggcgca
      961 gccccgaggc cagcgccaaa ctcaaaaaat ccgcccgcgc gctcactggg cacctccttt
     1021 cacgggcgcg ctcgcatcga gcgcggcttg cagcccacgc cgcagctcat cgagttgctg
     1081 tcccacctgg cgcgcggaga ggatcgtctc cgcgtcatag ctcggcgtct cctctagcag
     1141 ctccttcacg cgccggcgga atttcaccag ctcgcgcatc tccttcagtg ttggctcctt
     1201 catgtttcct ccttggttgt gtgtgcttgg gttgtgtttc acccggccta agcaggcaaa
     1261 aggggtcagg ccgcttgttg cgccggcggc agttgcagca gcgtcacatt gcccgtattg
     1321 agcctctccg tgatgatgcg gatcatcccc tggcgcatga ggtcagagat ggagactccg
     1381 gtttccaagc tgactttgct ggccgcctcg tccagttcgg gcggcagagt gaccgtgatg
     1441 cgtgtggctg attttttcat tgcgttatca ttgttaacca tgataacgca cccagcaagc
     1501 aatttttgat gacaaagtta actttgtgat gcagattccc tcatggcaaa aaaagacgcc
     1561 cgaattctcg gcatccggct cgacgaaaaa gacgccaccc ggctgcaaac cattgaagac
     1621 accctgtgca tcacaggcgt ctctctcgcc cgcgcggcgc tcaaagcagc gctcgaccat
     1681 tgggaaactc acggcaccat cacactgccg ctcgagatcc gccccaagtc agccgcgttc
     1741 ctgctcaacg aagagccaaa gaattacccg ccacgcgcgc acgatccagg agcgcagacc
     1801 ccgaagcaga gagccggcta gacacgccag cgtcagacaa catcatcccc ggcccgtggt
     1861 gatcccacgg gcctttttca ttccctgaaa ccacttctgg gagcaggcgg tgcctgcgac
     1921 cgccgttgcg cgtgcatcag cagctcatcg agctgctcat tgagccgttg ccgccctgtg
     1981 caaccaggaa aaagccatcg ccatgcccgg ttgctgaatg cagactagcg gtgcatcttc
     2041 acaagccagg ccgccatgaa tcccgacact cccttgacct acaagatgcc ccgccgcgtc
     2101 aaaagcggcg gcagcggctg cgccgccctc gtgtttttcc tgctcgccct cgcctgcgga
     2161 gcagtcggcc tctttgcctt ctggcccgcc ctcctgcccg caggcatcct cctcggactc
     2221 gccatcctga gcgactcaaa aacccgcatc gagcaccatt gcgggcattg cgggaacccc
     2281 gtcgcatcca cctcccggag ctgcccccac tgccgcgccg atctcctcac cctcccctgg
     2341 caccgccgca tctggcattg atcatgcacg ccatccacat ccccctcgcc gcagccctcg
     2401 gcctcatcgc catggccacc cttgtgccag cgcaaaaaaa cgtcggcatg tgccagccct
     2461 acgccgagac ctgcccccat tgcaccgact gcacccaatg cggccactgc gccaaaaaag
     2521 gcggccgctg cagcgtctgc tggccgcgct aatcccgcgc ccggttcagc ctggtgcgga
     2581 tccactcgct gaggtcaaga ccggcgcgct ccgccttcag cttccaccct cccttgtccg
     2641 ccgcgtggca gcggatgcgc agcacggtgt cgtagccgtc atccttcgcc gcattttgat
     2701 ttcccgccgg cgcgccgcgc ccgcttgccg tgtcttcgtt cgtgctcatt cccaggcttt
     2761 gctgaattgt tgatctttga agagggccgc aaggccggtg atctgtttga ggcgcagcag
     2821 ctcgtcggcg tccatgccga tgtttttcat aatccacgcg tcgcccatgc cgcaatccac
     2881 cagctcggcg acgatgccgc tcatgaggcc gatgtcatgc gtgccgcgcg cgcggttgtg
     2941 gcggatggtg gcggccatgc gattgctcag gtctttgttg atcaacacca ccggcaagtg
     3001 gccatgcact cgctcacgaa ttttctttga ctccttgccg atgcggccgc gatgaaagcc
     3061 gtccacggta gtcttgaccg gcttgtccgc ctcctgcatg gcgacgatgg gctgggtata
     3121 gccatcagcc tcgatgctgc gctccagcag tttcatctca ggaggggcga cgcggttcgg
     3181 attgtagtcg ttcgcggtga tgtcgtcaca cgggaaccac agaacgcaat cgaccggctc
     3241 tgccttgaac gggctgatct cgtgcagcgc gatcttcgtt tggttgatct ggtggatctg
     3301 ctcgcgctgc ggcagttcgg cgatggccgc gcacagcgcg cgccggagac gctcgatttc
     3361 aggttgctgg tcaaacaggt cgctcatgat ttggtgtgtt cgagttgggt gaatttccct
     3421 ttgggcagat gccctttgaa tccggccttc ttcgcgccgc gcacaacggt ctcggacttg
     3481 gtgacgatca ctgccgtgcc aggctgcatg tgggcaatga cggccttcgc gagagcggcg
     3541 gcggcctgct catgcatggc ataaaaactg ccgacatcga gcttcgacgg acgctgccag
     3601 gcgtagcaaa agccaacaac ctggccgtca tcgagcgcca ggtgccagac cttgccatcg
     3661 tcatcgtaga gcgggtagcc gatggccttg atcaccgtgc ggttggcgag aaacggcccc
     3721 atgcgcggat aaaactcatc gtcggtgttg gtcagttgga tcgtcttcat ggtcacaagt
     3781 ctttgtattt tgccagcaca gcagcacggc gctgctgctc ctcctttgtc agcgtgaaac
     3841 ccatgtattt gcacaggtga tcgttcttca gaatgcagac caccatgcgc ttgtagctgg
     3901 gcacggcctt gaactcggtg atgtccagct cgtcaggata gtcctggaac accacggtct
     3961 gcttcaccgt tttgtaggat gattcctttt tgatctcaac cttctcgccg gtgcgccgga
     4021 tgtcctccac cacgtcgtca tccagcacgc ctcctcgtgt ggtccagaac tccttgctca
     4081 cgcggaactt ttcctcgtag cgctgccgcg tctccgccgg cagtgtcgcc agcagaaact
     4141 cgagatacga cttccaagtg tgatgcttgg gcttggtgat ggacttccaa cccatcgccg
     4201 tcgtgccgcc gtagatgccg ctgaagttca ccccgttcac ccggctgacg agcttgcccc
     4261 aggtgtgcgg ctccacggcc cggtagagct tcagactctc aatgccctga ctgataaacg
     4321 gactcgccac ccgcatctgc ggcagcgtga gaccggcctg atggaagagg tcgtagaggc
     4381 ggttgtaggg cttgccaaag cgaccgtggt aaatccacac gtcatcgacg gaccagtcat
     4441 gaatgggata gaaattgaac tggcgcccgc tgtcgtcaat gccgcccgtg ctccagagga
     4501 tgccgggata cttgcgggcg ttgaccgtgt ttgccagcat gatgcgccgg tcgtagctct
     4561 cctcagcgcg aacgccgatt aggcaggccg ttttcttccc cgttgtgcgt gccacccagt
     4621 caccgaactg cttttgaaaa tcgtagtcct ccatgccgaa ccggtaaaaa tcgaatgagt
     4681 gattgcccag gtggatgctg ccctccggca aatcgcgcac ccagatgtca cgctcagccg
     4741 ggttccaggg cacccaggtg ctctggctca tgctggtgca gcacggcacc gtgagaggca
     4801 tggcgcacca atatttatca caccactcag gcaaggcatc gaaggtctca cgaacgtggt
     4861 ccgtggtggc ctggtattga gcctcgtagt cgatgtgaaa gacgccgata cgacggttag
     4921 ggcagtgctg gcggacgtag tccagcgcca gatgaaggat gacgctgctg tctttgccgc
     4981 cggagaacga gacatagaca tactcgaact ccgcaaacac cagagccagc cgctcctggg
     5041 cggcctgcag cacgttcttg ccagtcatgc gcttgatcat gccacgtcct ccagcaacat
     5101 cgccgccgcc agacagcgct caaaatccgc cagccaatgg acatcctcac gcgcggtcgt
     5161 cagcaagccg ggcaggagca ccacggcaag ccagcgcccc gcaggcggac gcgccttcac
     5221 cggcgcaaac cgagcctgta gatcgcgcag cgtgctccac acgtcatcct caccctgacc
     5281 tagcccgcat gtggcgatgg gttcacgtcc gcgccagatc gtaaacaccg gcgcatggat
     5341 ttccacccgg aacgccggga atccaggtat ctgcccaccc tcgggcagca gctcccggca
     5401 ttgctcgacg acttcaggcg gaataatgtc cagcctgtgc aggacattat cccccgtcat
     5461 gagtgtgatg tggttgatgg tcatagcgct ttgatcgcct gaataatggt ctttttgtcc
     5521 cgcatgcgct ggatttcagc acgaaattcg ttcaccacat cgcctttttt cgccaggcaa
     5581 ttctggatgc ggtcatcaat ggtgccgctg caatgcaagt caatgtaggt cactttccgc
     5641 gcctggccga tacggtggca gcgatcctca ctttgcagcc ggttggcaaa cttgaactgg
     5701 ttgtcgtagt aaacggcata cgaggcctca gtcaggttca ggccatgccc gccacaatcc
     5761 ggcgtcgcca ccagataccg ccgggaggat cggaatgaat ccaactgccg ggtgcgctcc
     5821 gactcggaaa ggcctccatg aaacaccgcc acagacgcgc caaggctcgc ctcgatctgc
     5881 gccaggctcc tcctgtgctt cgtccagatg atcacttttt caccttccgg gatctccccc
     5941 agcacagaat ccagcagctc gagccgattg tgcggaaact ccagcaactc ggcttcctca
     6001 tccttttccg atttccggtt ccaccagccg ctcacgatgc actgcagcgc attgaacagc
     6061 cggaaaatcg tcgctgcggt cacttcaccc tcattgtcga gatactcctg cagcatctcg
     6121 tctttggccc gctcataggc ttcccgctgc tctggagtga gcgtgcagta gcgcgtggtg
     6181 taaagcttgt ccggcagatt cagacactcc tcttttgtca cctggtaaac gtagggagcc
     6241 atctttgccg ccagccaatc cgtgttgtgc gcccgcacga tcatgcccgg aaacttttcg
     6301 tgatactcca gatggtttgc agcgaagctg tgaaaacttt tgtagcccag aatcttggtg
     6361 ctcaggaacc tcatctgagc gaacaaatcc accaccccct ggctgatcgg cgtgcccgtc
     6421 aaaatcagcc ggtagcgcgc ccgggccgcg attttggtga cgtaggtggt gcgcctggcg
     6481 cgatgccctt tgatgtagct gctctcatcc acgatcacca tcgcatttgc atcaatcagc
     6541 gactctgccg ccacgatcac ccgcacgctg gctgacatgc tttcgagccc gattacatac
     6601 caccgagcct cgggcatctt gccctcgcag gttttgtgat caaacacatg cactgtctgc
     6661 ccaggccagt gcttttcaat ctcccgccgg atcgtctctt tcacagacac cgggcagaac
     6721 caaagcaccc ggctgatccg gtcccggcgt tgcgcggcga tctcaatcgc cgtccggctt
     6781 ttgccggtgc ccatctccat gaacaatgcc cccacacggc tgcggtggag ttttgccacc
     6841 gcagccgcct ggtgctgcat cagcggagtc accggagcat cagtcatcgc gcagatcatc
     6901 gtccacctcc ccggttgcag gttccatttc attcacaggc ttttccatct ccggttccgc
     6961 gccgagctga accttcagcg acctcgcatc cgcttcacgg cccgctgcca ccgccttctc
     7021 ggctccgggg ctgagccgga atccctgcct ttcggcgaaa tcctcgacgc tttcccacgc
     7081 ctccaatggc accagcatcg aacgcaactc tttgctccac ttggcacctg gcagacgctt
     7141 cgcctgttca taaaaatcat ctcccgattc acgatcccac tgcacgcgga actttttttg
     7201 atccggctgc caaaacaccc ggcgcgtcgt gtgcggacgg aactgccccg ccgccacgcg
     7261 catccgcact tcttccggag ccaagcgcac gcggaacccc gccttgagca gacgcc
//
LOCUS       DAOMYJ010000246         7294 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Prosthecobacter sp. isolate AMR_MDS_5629
            NODE_3431_length_7294_cov_20.840448, whole genome shotgun sequence.
ACCESSION   DAOMYJ010000246 DAOMYJ010000000
VERSION     DAOMYJ010000246.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867850
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prosthecobacter sp. (wastewater metagenome)
  ORGANISM  Prosthecobacter sp.
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Verrucomicrobiaceae; Prosthecobacter.
REFERENCE   1  (bases 1 to 7294)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7294)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.30
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:34:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,742
            CDSs (total)                      :: 4,684
            Genes (coding)                    :: 4,676
            CDSs (with protein)               :: 4,676
            Genes (RNA)                       :: 58
            tRNAs                             :: 55
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7294
                     /organism="Prosthecobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3431_length_7294_cov_20.840448"
                     /isolate="AMR_MDS_5629"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1965333"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..373
                     /locus_tag="PLP58_18980"
     CDS             <1..373
                     /locus_tag="PLP58_18980"
                     /inference="COORDINATES: protein motif:HMM:NF015534.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="Type 1 glutamine amidotransferase-like
                     domain-containing protein"
                     /protein_id="HRJ10673.1"
                     /translation="AGGAVIGGTSAGAAAMSQIMIEGTGDAGGISAGAVKISEGLGLL
                     PGVIVDQHFFKRQRFNRLLAAVREHPGRTGAGIDEGTALIVTDREWRVAGVSHVLILR
                     ADGSSGWNMRLLAAGDTMPAP"
     gene            422..1744
                     /locus_tag="PLP58_18985"
     CDS             422..1744
                     /locus_tag="PLP58_18985"
                     /inference="COORDINATES: protein motif:HMM:NF013567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HRJ10674.1"
                     /translation="MNRRRFLRQSAAATLGFPAILRSASPNSLLQVAAIGVGGMGGRT
                     LQSVARHEKVKVTALCDVDAAYLGKAAAEFTGASTHRDWREMLASHAGKFDAVTIGTP
                     DHMHAAPAVLAMRAGKHVYLQKPMATTLHECRVITREAAKAGVVTQLGNQGRSSIESR
                     MTVQLLRSGAIGKVREIIMWENKPLSWWPKNTTLRPQADPVPGTLDWDLWCGVREPVP
                     YLENTYHPKNWRAWFDFGCGEMGDMGCHHLDASFDALKLAAPIRARLTHGGSSGPLWG
                     EKRVVELDFPGSEFTADETIRLTWHDGGVEPDRSKISMPASLKQFPRSGAYWVGTQGG
                     MLKPYGVRPFVVPEESFPNEKYPRGIPGQDHYFDWVDAILQGRKSCADFSHGGPLTEA
                     VLVGAFTDRFPGQWLEWDREACAISNHTGASALVKRAYRDGWQVPDLG"
     gene            complement(1767..2126)
                     /locus_tag="PLP58_18990"
     CDS             complement(1767..2126)
                     /locus_tag="PLP58_18990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009962476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HRJ10675.1"
                     /translation="MLVLHTYQKCSTCREAVRWLRAHGIPFEEHPIREEPPSLETLRR
                     ALADMGGQLRALFNTSGQDYRALGLKDKLPAMSADEALRLLAGNGNLIKRPFARDDKR
                     GITLAGFKAEAWSAALL"
     gene            2248..3843
                     /locus_tag="PLP58_18995"
     CDS             2248..3843
                     /locus_tag="PLP58_18995"
                     /inference="COORDINATES: protein motif:HMM:NF037997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005436 - sodium:phosphate symporter
                     activity [Evidence IEA];
                     GO_function: GO:0015114 - phosphate ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0035435 - phosphate ion transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0044341 - sodium-dependent phosphate
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na/Pi symporter"
                     /protein_id="HRJ10676.1"
                     /translation="MIDASFHLAGGLGLFLLGMALLTEGLRVMAGETLRRALVRFTGP
                     PLKAFGSGALVTLLAQSSSATTVTVIGFVSAGLLTFPQAIGVVMGASLGTTGTGWLVA
                     LLGLKIKLGLYALPLVGLGAFLRLLTHGGRAAFGTALAGFGIIFLGIDFMQEAMAELS
                     KVFRPADLPHDFTGHLLAMLLGIGLTLLLQSSSAAMATTLTALHTGAIHFDQAASVTV
                     GAAIGTTVTGVLAALGGSVPAKRTALAHVIFNLATGLLALSMLPWYLRAISHAQARWG
                     LDTGAVSLAAFHTAFILAGVVLFLPWTRPFARGIERLLKDKGPRLTRHLDKSLLHTAP
                     VALEAVRRSLSETACELFRALRRCLDASAAPMAEAQRMEITSALRETQHFIAKIPVRP
                     GNEKLSLARISELHALDHLLRLQPRLDAPAGVLEMLRHERLKEAAAMTGAILEKAVSG
                     LIAGGPASRWVAEIETQSTALAALRRQERAAILRQTAGGDWTPAKSLEMLDALHWLDH
                     ASYHAWRICHYLADGENGAGPPS"
     gene            4162..>7294
                     /locus_tag="PLP58_19000"
     CDS             4162..>7294
                     /locus_tag="PLP58_19000"
                     /inference="COORDINATES: protein motif:HMM:NF039815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ10677.1"
                     /translation="MRRFSRRAWWLHAFLIAQLTLLPCPQVSHAVWVEVDTDGDGIAD
                     YGYEDGTGDPPPGEDPPAWEPGPVEDSDGDGLTDEQEAILGTDPYNPDSDYDGLTDAD
                     EIHLTGTNPLSPDSNGDGVSDFNEFYGNYSVNTHVHGQGATPYDWDGDGIADPDDPDP
                     FSPDNHADSDGDYVPDHEDSHPYEPWLWNDWNSNGVNDDAEDPAADSDGDGISDATDS
                     HPWDPVLYNDWNGNGENDEHEDWDGDGVSNLQDSHPDNNTLWCDWNGNGVNDDAEAGL
                     MDQDGDGHEDTNDSHPFNPALWSDWNHNGVNDPDEAGAPDPATTDSDGDGHFDSADSH
                     PFDPALHSDWNHNGVNDLDEIWTSDTSTTDSDGDGHPDAFDSHPWDSVLWSDWNHNGA
                     NDENEPAPADPDSDGDGHPDSADTDPHDATLWEDHNLNGINDSLEELVTDSDGDGITD
                     PHDTHPHDPALWNDWDYNGVNDDQEVLITDSDGDGYADHLDTHPEDASLWNDHNDDGI
                     NDEHQTPPDSDNDGIPDPLDEFPHDFDNDGLPDAEELLWGTDPALADTDGDGLSDGDE
                     IYAGTDPLNVDTDADGLTDFEELRTYHTDPLTPTPLSPDGQAPEGHAPPDEEEEGPVA
                     GDASGGGASTADQPAFNGIWNAAKNRAWFADLTDSDGDGIPDKVEQMYYPLIVSPDGD
                     LDGDGMTNLAQYNLGKDLRGNNSSTDFDGDGLTDAVEDAWAKAYPDSVTLSKYRFADA
                     FEDPDGDGLLTIEELQASWGGKKEPNAVATHPFVKSSIPNTNSKSPTYKTTTRNPPPN
                     TAAGQNPAHFSQRSGIYADWFDDGLLRLAIRQSTLPNGKLPSTFFNRSHLVHAATTAQ
                     QTLSGSDHLPLGYVQWLAVRGIAPPQQPAMPLPPPPPQAADATKLQALSPPSAGDADH
                     DGMPDAWEAACGLNWRSLFDSGPGYHPREQLEQAAAAFLASTTFPDTSLKLLWIDLYA
                     QASQTLVDGASGGPPEIETELLFHSPHHEEMFYQSLVPEYPLGAPAVPPKPAASAASA
                     VHQALPFPLASPSK"
BASE COUNT         1348 a         2754 c         2088 g         1104 t
ORIGIN      
        1 ggccggcggc gccgtcatcg gcggcaccag cgcgggcgcg gccgcgatgt ctcaaatcat
       61 gatcgaaggc accggcgacg ccggtggcat ctccgccggg gcggtgaaaa tcagcgaggg
      121 tctcggactg ctgccgggtg tcatcgtgga ccagcatttt ttcaagcgtc agcgcttcaa
      181 ccgcctgctc gccgccgtca gggagcaccc cggccggacc ggcgcgggca tagacgaagg
      241 cacggcgctc attgtcacgg accgggagtg gcgggtggcg ggagtctctc acgtcctcat
      301 cctgcgcgcg gacggctcca gcggctggaa tatgcgcctg ctcgccgcgg gtgacaccat
      361 gccggcgccc tgacgacaac atccgcaaag tccgcccggc gcggcgcgtt ctcctgctcc
      421 catgaaccgc cgccgtttcc tccgccagtc cgccgccgcc acgctcggct tccccgccat
      481 cctgcgctcc gcctcgccca attccctgct ccaggtggcc gccatcggag tcggcggcat
      541 gggcgggcgc acccttcaga gcgtggcccg gcatgaaaag gtgaaagtca ccgcgctctg
      601 tgatgtggat gccgcctacc tcggcaaagc cgccgccgaa ttcaccggcg cctccaccca
      661 ccgcgactgg cgcgagatgc tcgcctccca tgcgggcaaa ttcgacgccg tcaccatcgg
      721 cacgcccgac cacatgcacg ccgcgcccgc ggtgctggcc atgcgcgcgg gcaagcatgt
      781 ctatctgcaa aagcccatgg ccaccacgct gcacgagtgc cgcgtcatca cgcgggaggc
      841 ggcgaaagcg ggtgtcgtca cccagctcgg caaccagggg cgctccagca tcgagtcccg
      901 catgaccgtg cagctcctgc gctcgggcgc catcggcaag gtcagggaaa tcatcatgtg
      961 ggagaacaag ccgctgagct ggtggccgaa aaacaccacc ctcaggcccc aggccgaccc
     1021 tgtgcccggc acgctcgact gggatctctg gtgcggcgtg cgcgagcccg tgccctacct
     1081 ggagaacacc taccacccca aaaactggcg cgcctggttt gacttcggct gcggcgagat
     1141 gggcgacatg ggctgccatc acctcgacgc cagctttgac gcgctgaaac tcgccgctcc
     1201 catccgcgcc cgcctcaccc acggcggcag cagcggcccg ctctgggggg agaagcgcgt
     1261 cgtcgaactc gacttccccg gcagcgaatt caccgccgat gagaccatcc gcctcacctg
     1321 gcatgacggc ggcgtggaac cggaccgctc caagatttcc atgcccgcct ccctgaaaca
     1381 gttcccccga tccggagcct actgggtggg cacccagggc ggcatgctca aaccctatgg
     1441 cgtgcgcccc ttcgtcgtgc ccgaggagag ctttcccaac gaaaaatacc cgcgcggcat
     1501 tcccggccag gaccactact tcgactgggt ggacgccatc ctccagggcc gcaaaagctg
     1561 cgccgacttc tcccacggcg gcccgctcac cgaggccgtg ctcgtcggcg cgttcaccga
     1621 ccgctttccc ggccagtggc tggagtggga ccgcgaagcc tgcgccatct ccaaccacac
     1681 cggagccagc gccctcgtca agcgcgccta ccgggacggc tggcaggtgc cggacctcgg
     1741 ctgacattgc gcgccaggcg cgcggttcag agcaaagccg cgctccacgc ctcagcttta
     1801 aagcccgcga gagttatgcc gcgtttatcg tcccgcgcga aggggcgctt gatcaggttg
     1861 ccgttgccgg ccagcaggcg caaagcctcg tcggcactca tggcgggcag tttgtccttg
     1921 aggccgagcg cgcggtagtc ctggccggag gtgttgaaaa gcgcgcgcaa ctgccccccc
     1981 atgtccgcca gcgcccggcg cagcgtttcc agggatggcg gctcctcgcg gatgggatgc
     2041 tcctcaaacg gaattccgtg cgcgcgcagc cagcggactg cctcgcggca ggtggagcat
     2101 ttttgataag tgtgcaggac aagcatggcc gcgcagccaa gagcattcgg aagtctttgc
     2161 aaggccgcgt ctcctcctgc gcggttgccg ctgtcaaaag gttgagcgga agggtgacaa
     2221 gcggcgttcc cggactgagc ttgcaaaatg atagatgcct ccttccatct cgccggcggt
     2281 ctgggattat tcctgctggg catggcgctg ctcaccgaag gcttgcgtgt catggcgggc
     2341 gaaacgctgc gccgcgccct ggttcgtttc accgggcccc ccctcaaagc cttcggctcc
     2401 ggcgcgctgg tgacgctgct ggcgcagtcc tccagcgcca cgacagtcac cgtcatcgga
     2461 ttcgtcagcg cgggtctgct gacgtttccg caggccatcg gcgtggtcat gggcgccagc
     2521 ctgggcacca cgggcacggg ctggttggtg gctctgctcg gcctcaagat caaactcggc
     2581 ctctacgcgc tgcccctggt tggactgggg gcttttctgc ggctgctgac acacggaggg
     2641 cgagcggcgt ttgggacggc gctggcaggc ttcggcataa tcttcctggg catagacttc
     2701 atgcaggagg cgatggcgga gctgtccaaa gtcttccgtc ccgccgacct gccccatgac
     2761 ttcacagggc acctgctcgc catgctgctg ggcatcggcc tcaccctctt gctgcaatcc
     2821 tccagcgccg ccatggccac caccctcacc gcccttcaca ccggagccat ccactttgac
     2881 caggccgcct cggtgacggt cggcgcggcc atcggcacca cggtcaccgg tgtgctggcg
     2941 gcgctcggcg gcagcgtgcc ggcgaagcgg acggccctgg cccatgtcat cttcaacctg
     3001 gccaccggcc tgctcgccct ctccatgctc ccctggtatc tgcgcgccat cagccatgcc
     3061 caggcccgct gggggctgga caccggcgcg gtgagccttg ccgctttcca cacagccttc
     3121 atcctcgcgg gagtggtgct tttcctgccc tggacacgcc ccttcgcgcg cggcatcgag
     3181 cgccttctca aagacaaggg gccgcgcctc accaggcatc tggacaaatc cctcctccac
     3241 acagctcctg tggctctgga agccgtgcgg cgctcgcttt ccgaaaccgc gtgcgagctt
     3301 ttccgcgccc tgcgcagatg cctggacgcg tccgccgcgc cgatggcgga ggcgcagcgc
     3361 atggaaatca cttcagccct gcgggagaca cagcatttca tcgccaaaat cccggtgcgg
     3421 ccgggcaatg aaaaactctc cctggcgcgc atctccgagc tgcacgcgct ggaccacctg
     3481 ctgcgcctcc agccccggct cgacgcgccc gcaggcgtgc tggaaatgct caggcacgag
     3541 cgcctcaaag aggctgcggc catgacaggc gccatcctgg agaaagccgt gtccggcctc
     3601 atcgcaggcg gccccgcctc ccgctgggtg gcggaaatcg aaacgcaatc caccgcgctc
     3661 gccgccctgc gcagacagga gcgcgccgcc atcctgcgcc agaccgcagg gggcgactgg
     3721 acgcctgcga agtccctgga aatgctcgac gccctgcact ggctcgacca cgccagctat
     3781 catgcctggc gcatctgcca ctacctggcc gatggcgaaa acggagcagg cccgccctcc
     3841 tgacgagcat tgggtccatc ttcgacaagt catcaacgtg tctgcggcaa gtgtccggtg
     3901 acaaaaggat ttgccggcag atgaaaagac cgggccaatt ttgccaccgc gcaaaaaaat
     3961 cctctcgaca tttctctccc cttctgatgg gatcgcttta attcgatcaa taaaagccga
     4021 gcgcgccaga ccattagtca tcccacgcct catgacctcc cccgcttcac ccatccccgc
     4081 tggtatccag gcttctgccg cacagccatc agccatcagc catcagccat cagccatcag
     4141 ccaacaactc cccttgagtt gatgaggcgt ttcagccgtc gtgcgtggtg gctgcacgcc
     4201 ttcttgatcg cccagctcac tttgcttcct tgtccgcagg tttcccatgc ggtctgggtg
     4261 gaggtggaca ccgatgggga tggcattgcc gactacggct atgaggacgg cacgggcgat
     4321 cctcctcccg gggaggaccc gcccgcttgg gaacccggtc ccgtcgagga ctctgatggg
     4381 gacggcctca ccgacgagca ggaggccatc ctcggcaccg acccctacaa tcctgactcc
     4441 gactacgacg gcctcaccga cgccgatgag atccacctca ccggcaccaa tcccctcagc
     4501 cccgacagca acggcgacgg cgtctccgat ttcaacgagt tctacggcaa ttactccgtc
     4561 aacactcatg tccacggcca gggagccacg ccctacgact gggacggcga cggcatcgcc
     4621 gatcccgacg accccgatcc tttttccccc gacaaccacg ccgactccga cggcgactac
     4681 gtccccgacc acgaggattc ccacccctac gaaccctggc tctggaatga ttggaattcc
     4741 aacggcgtca atgacgacgc cgaggacccc gcggccgact ccgacggcga cggcatctcc
     4801 gacgccaccg actcccaccc ctgggacccc gtgctttaca acgactggaa cggcaacggc
     4861 gaaaacgacg agcacgagga ctgggacggc gacggcgtga gcaatctcca ggactctcat
     4921 ccggacaaca acaccctttg gtgcgactgg aacggcaacg gcgtcaacga cgacgccgag
     4981 gccggcctca tggaccagga cggcgacggc cacgaggaca ccaacgactc ccaccccttc
     5041 aatcccgccc tctggagcga ctggaaccac aacggcgtca acgaccccga tgaggcgggc
     5101 gcgcccgacc ccgccaccac cgacagcgac ggcgacggcc acttcgattc cgccgacagc
     5161 catcctttcg atcccgccct gcacagcgac tggaaccaca acggcgtcaa cgacctcgac
     5221 gagatctgga cctccgatac ctccaccacc gattccgacg gtgacggcca ccccgacgct
     5281 ttcgacagcc atccctggga ctccgtcctg tggagcgact ggaatcacaa cggcgccaat
     5341 gacgagaacg aacccgcccc ggcggacccg gacagtgatg gcgacggtca ccccgactcc
     5401 gcggacacag acccccatga cgccaccctc tgggaggacc acaacctcaa cggcatcaac
     5461 gacagcttgg aggaactcgt caccgacagc gacggcgacg gcatcaccga ccctcacgac
     5521 acccacccgc acgatccggc cctttggaat gactgggact acaacggcgt caacgacgac
     5581 caggaagtgc tcatcaccga cagcgacggc gacggctacg ccgaccacct cgacactcat
     5641 ccggaagatg ccagcctctg gaacgaccac aacgacgacg gcatcaacga cgagcatcaa
     5701 accccgcctg acagcgacaa cgacggcatc cccgaccccc tcgacgaatt cccccacgac
     5761 ttcgacaacg acggcctgcc cgacgccgag gaactgctct ggggcaccga ccccgccctg
     5821 gccgacaccg atggcgacgg cctcagcgac ggcgatgaaa tctacgccgg caccgacccc
     5881 ctcaacgtgg acaccgacgc cgacggcctc accgacttcg aggagctgcg cacctaccac
     5941 accgatccgc tgacccccac gcccttgtcc ccggacggtc aggcgccgga gggccatgcg
     6001 ccacccgatg aagaggagga aggccccgtg gccggagacg cttccggggg cggggcctcc
     6061 acggctgacc agcccgcgtt caacggcatc tggaacgccg ccaaaaatcg cgcctggttc
     6121 gccgacctca ccgatagcga tggcgacggc atccctgaca aagtcgagca gatgtattac
     6181 cccctcatcg tcagcccgga tggcgacctc gacggcgacg gcatgaccaa cctcgcccaa
     6241 tacaacctcg gtaaagacct gcgcggcaac aatagcagca cggactttga cggcgacggc
     6301 ctcacggacg ccgtcgaaga cgcctgggcc aaagcctacc ccgactcagt caccttgagc
     6361 aaataccgct tcgccgacgc cttcgaagac cccgatggcg acggcctgct caccattgag
     6421 gaactccagg catcctgggg cgggaaaaaa gagcccaatg ccgtcgccac ccaccccttc
     6481 gtcaaaagca gcatccccaa caccaacagc aaatccccca cttacaaaac caccacgcgc
     6541 aacccgccgc ccaacaccgc cgcaggccag aaccccgccc acttttccca acgaagcggc
     6601 atttacgcgg actggttcga tgacggcctg ctgcgcctcg ccatccgcca gtccaccctg
     6661 cccaacggca aactgccctc caccttcttc aaccgctccc acctcgtcca cgccgccacc
     6721 actgcccagc agaccctcag cggctccgac cacctgcccc tggggtatgt gcaatggctc
     6781 gccgtccgcg gcatcgcccc gccccagcag cccgccatgc ccttgccgcc acccccgccg
     6841 caagccgcag acgccaccaa gctccaggcc ctgagcccgc cctctgccgg agacgccgac
     6901 cacgacggca tgcccgacgc ctgggaggcc gcctgcgggc tgaactggcg cagcctcttt
     6961 gacagcggcc ccggctatca tcccagagag cagttggagc aggccgcagc cgccttcctg
     7021 gcctccacca cctttcccga cacctcgctc aagctcctgt ggattgacct ctacgcacag
     7081 gccagccaga ccctcgttga cggcgccagc ggcggcccgc cggagatcga gaccgaactc
     7141 cttttccact ccccgcacca cgaggagatg ttttatcaaa gcctcgtgcc cgaataccca
     7201 ctcggcgcgc ccgccgtccc gcccaagccc gccgccagcg ccgcctcggc cgtgcatcag
     7261 gccttgccat tccccttggc ttcgccttcg aaat
//
LOCUS       DAOMYJ010000247         7266 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Prosthecobacter sp. isolate AMR_MDS_5629
            NODE_3443_length_7266_cov_25.272500, whole genome shotgun sequence.
ACCESSION   DAOMYJ010000247 DAOMYJ010000000
VERSION     DAOMYJ010000247.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867850
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prosthecobacter sp. (wastewater metagenome)
  ORGANISM  Prosthecobacter sp.
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Verrucomicrobiaceae; Prosthecobacter.
REFERENCE   1  (bases 1 to 7266)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7266)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.30
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:34:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,742
            CDSs (total)                      :: 4,684
            Genes (coding)                    :: 4,676
            CDSs (with protein)               :: 4,676
            Genes (RNA)                       :: 58
            tRNAs                             :: 55
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7266
                     /organism="Prosthecobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3443_length_7266_cov_25.272500"
                     /isolate="AMR_MDS_5629"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1965333"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..401)
                     /locus_tag="PLP58_19005"
     CDS             complement(<1..401)
                     /locus_tag="PLP58_19005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ10678.1"
                     /translation="MSSDPPEDSQASPPLAGEEVSAGIEAETEKTLRRENRRVLLLVC
                     VVAAFMLLAHFTPLGAWITEVREWKEMLRAMGLKGEGVFAVACAAGVMLGLPRLPLCA
                     AAGLLFGFGEGLLASLSGAVTGSYGAFLLARA"
     gene            544..1287
                     /locus_tag="PLP58_19010"
     CDS             544..1287
                     /locus_tag="PLP58_19010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ10679.1"
                     /translation="MGKLFPTLRTLQHTGHLVQVRRSTNARGVTTTRTFDLAGRLTVI
                     SYDDDSTPAFFYQLDHRDRATAVFNGNDDSEGALYAYTYNGPGGVWDQMFWEGGSVTR
                     TFDDHARLHQLMVKQGEDVLFTTTHSHDPATGGLASVTHGGVTAMYDWTAPIGGLPGA
                     VRFRRWSSDLFNRDYKSTTHMEVMRTKTSAMIEKEPLMHAIAYNALRALILESATVHQ
                     QELGRTRFASQQRHGGPAPAMACPSRRLS"
     gene            complement(1554..2441)
                     /locus_tag="PLP58_19015"
     CDS             complement(1554..2441)
                     /locus_tag="PLP58_19015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ10680.1"
                     /translation="MKRELLRIATTLGCIILGAQALRADSTISATLKHAYSVNAGWIN
                     LRPDQPVSPTGIVLGGSFISGHAYGTNIGWIDFGDGTPSNGFSYQNNSATDYGVNHDG
                     AGNLGGMAYGANIGWINFGWAAPASPNRPRVNLLTGVFSGFAYSVNLGWINLGGGSLV
                     TTAMRVTDSDSDGMDDAWERARFPSLATASATSDTDKDGVSDANEYLAGSNPNSDTDY
                     FKVVSAIFGEENIQATVTFTTDPARLYRIETSDNLVNWQNSTHGTFAPDPGTTSTRVV
                     TWTGNDRRFIRAVAVRPLP"
     gene            2574..4016
                     /locus_tag="PLP58_19020"
     CDS             2574..4016
                     /locus_tag="PLP58_19020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ10681.1"
                     /translation="MTVTFCPGQGTLTPPGPPGVTMKTLDEVEPRTPINSLTTPGDSG
                     AAFKITQPGSYVLTGNLTPASGKHGIQVTLATSGRVEIDLMGFTINGASAGAGKSGIM
                     IDAGAGNDSLCSYMIKNGSLRAFSAANIKVDPACLGDTATHEVGHWAGLTAVESAGRV
                     TITGGRIQDPTGSAIIAGAESRFTGLEIRDSSSGPDPIIVASGGNFSLDDVLVSSVSG
                     GNSNQPAILLGPGSKIRGMTATFSGGTYTGTIVGTTSGFMDVTGLDIVMTGVTAASGV
                     SSLQTSIQRPYEFSTTGSADGSTVQWPLVIRAVNSTFSFAIDVVQSGLSLPSSDHCPR
                     ILLEGTTTTPAAFSLESSNHCPAIMDIGIASTASVTGAVVQVTGSGNTIRANIRGIPG
                     GGTGIRFFSGSGNTVESSTFIGRGDSAITAVRLDNGVTNTLVRNNRGSRWFGGSVMVN
                     NLGDNTNAIAPVGNSTNLSTNSNPMSNFVQ"
     gene            complement(4529..4840)
                     /locus_tag="PLP58_19025"
     CDS             complement(4529..4840)
                     /locus_tag="PLP58_19025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ10682.1"
                     /translation="MTLLITLRFSISLANYQSHTCKCRRCHKTGCAMLTCLFQAVRAV
                     ADARRVIRSRARGTNTRYHASKKKYTKGNQASYCIANDWFLIPDYMLKNQYSSQDDKQ
                     S"
     gene            4839..5255
                     /locus_tag="PLP58_19030"
     CDS             4839..5255
                     /locus_tag="PLP58_19030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ10683.1"
                     /translation="MKMKPLVFCCLAMLLAILPAQGESKHMMKITTTTEGDLFADSEH
                     EARTREWTPLSDWIVPQNNLALIAQASLQEKHGSRMKVFNAELTHVFWTRSFEGWWFL
                     QIQFLIRSKESSPCSGAEMKSIYLLPDGGLLRLVQK"
     gene            5670..6434
                     /locus_tag="PLP58_19035"
     CDS             5670..6434
                     /locus_tag="PLP58_19035"
                     /inference="COORDINATES: protein motif:HMM:NF012730.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005216 - monoatomic ion channel activity
                     [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ion transporter"
                     /protein_id="HRJ10684.1"
                     /translation="MKSADSQGLGRAEKAIQVVIILSLVTFALETLPGLLPWQRRALA
                     AFEMFSIAVFTIEYLVRLSLARPVSKYAFSFFGIVDLLAILPFYLSAGFDLRSLRAFR
                     LLRLLRVLKLARYSRAMQRFHRAFIIAREELVLFGATACILLYLAAVGIYYFENAAQP
                     QVFTSLFDGLWWAISTLTTVGYGDAYPITAGGRFFTFFILIIGLGVIAVPTGLIASAL
                     SKARREEDEEEARALEAAAIAQTSPDSESPSGPGPA"
     gene            complement(6379..>7266)
                     /locus_tag="PLP58_19040"
     CDS             complement(6379..>7266)
                     /locus_tag="PLP58_19040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009958962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-linked oxidase C-terminal domain-containing
                     protein"
                     /protein_id="HRJ10685.1"
                     /translation="GLEAVLADGTVVKAGGRCHKNKTGFDLVGLMVGSEGLLGVVTEA
                     TLRLIPHPPMRAMLSAGFGSFAEAANAVGRILDSGYLPSALEIADKFTLRAAREYLGE
                     SVTPQGDAHLLVELDGHEASVKGELAALAELVRGLGCICLEEALGEAACERFWKLRRE
                     FSYSLRNTGLIKLNEDIVVPRSRLVDLVDFAETLQGEFGFPVACFGHAGDGNIHVNIM
                     VPTMDDPAIRERAEAALDKLFHQVIAWKGAITGEHGVGIAKERWFPHAVSEGARALHL
                     RLKQALDPKGILNPGKFVR"
BASE COUNT         1419 a         2288 c         2153 g         1406 t
ORIGIN      
        1 gcccgcgcca gcaggaacgc gccatacgag cccgtcaccg cgccgctcag ggaggccagc
       61 agcccctcgc caaagccgaa caaaagcccc gccgccgcgc acaggggcag ccggggcagg
      121 ccgagcatca cacccgcggc gcacgccacc gcgaagacgc cctccccttt caggcccatg
      181 gcgcgcagca tctccttcca ctcccgcacc tcagtgatcc aggcacccag cggggtaaaa
      241 tgcgccagga gcatgaatgc agccactacg cagaccagga gcagcacacg gcggttttcc
      301 cggcgaaggg tcttttccgt ctcggcttca atgccagccg acacttcctc tccggcaagg
      361 gggggggaag cttgggagtc ctcagggggg tcactcgaca cgggcgctgg cattccagcc
      421 tcatgacgcc atgccgccga cttctcaagc gacgaaatga ggcactcaac ggcaacacga
      481 aacgtgtccc gtttgaaaag tcacaactgc gccggctcct cgatacttca cggcccgcta
      541 aacgtgggta agcttttccc tacattacga acgctccaac acacgggtca cctcgtccag
      601 gtgcggcgct ccaccaatgc caggggcgtc accaccacgc gcactttcga cctcgcgggc
      661 cgcctcaccg tcatttccta tgacgacgat tccacgccag ccttcttcta ccaattggat
      721 catcgcgacc gtgccaccgc ggtcttcaac ggcaatgatg actcggaagg cgcgctttac
      781 gcttacacct acaacggccc cggcggagtc tgggaccaaa tgttctggga aggcggatca
      841 gtcacccgca ccttcgacga ccacgcccgc ctgcatcagc tcatggtcaa gcagggggag
      901 gatgtgcttt tcaccaccac ccacagccac gatcccgcca ccggcgggct ggccagcgtc
      961 actcacggcg gcgtcaccgc catgtatgac tggacggctc cgattggcgg cctgcccgga
     1021 gccgtacgct tccggcgctg gagcagcgat ctgtttaacc gcgactacaa aagtaccacg
     1081 cacatggagg tcatgcgcac caagacatca gccatgatcg aaaaagagcc gctcatgcac
     1141 gccatcgcct acaacgccct gcgcgccctg attctggaga gcgccactgt gcatcagcag
     1201 gagctggggc gaaccaggtt cgcttcgcag caaaggcacg gtggacctgc tccggcaatg
     1261 gcttgcccaa gccgccgcct gtcatgagca gccgcgaagc tggcccagtg acatgaggag
     1321 ctgctcgaag ccaccgccag cgtgcggaac ccgctacgcc ccggaaggta agaacccaga
     1381 gccaaaaagt gacgccccaa agtcctgcca actgctcacc aaccccccac aaattaaagg
     1441 gaatctcccg ccgcttaagt gagtgccatt cttgccaggc aacaatttct cgcacatgat
     1501 acatggagtg gggacgtatg agttcttgaa ggagaagctc ggcttgaatt gactcacggc
     1561 agcggccgca ccgccacggc gcggatgaag cggcggtcat tgcccgtcca ggtgacaact
     1621 cgcgtgctcg tggtgccggg atccggcgcg aaggtgccgt gggtggagtt ttgccagttc
     1681 acgaggttgt cggaggtctc gatgcggtaa aggcgcgcag ggtctgttgt gaaggtgacg
     1741 gtggcttgga tgttttcctc gccaaaaata gcgctgacga ccttgaagta gtctgtatca
     1801 gaattcggat tgctcccagc cagatactca ttcgcatcgc tgacgccatc cttgtccgtg
     1861 tcggatgtgg cgctggccgt cgcaagactc ggaaaacggg cgcgctccca ggcatcatcc
     1921 atgccatcgc tgtcactgtc cgttacgcgc atggcggtgg tgacgagcga tccgccgccg
     1981 agattgatcc agccgaggtt cacactgtag gcaaagccgc tgaacacgcc ggtgaggagg
     2041 ttcacacgcg gacggttggg agaggcgggc gcggcccagc caaaattgat ccagccgatg
     2101 ttcgcgccat aggccatgcc gccgagattg cccgcgccgt catggttgac gccgtaatcc
     2161 gtggcgctgt tgttttgata cgagaagcca ttcgaaggcg tgccatcgcc aaagtctatc
     2221 cagccgatat tggtgccgta ggcatgaccg gagatgaaag aaccgcccaa aacgatgccg
     2281 gtgggcgaca caggctggtc cgggcgcagg ttgatccatc ccgcattgac gctgtaggcg
     2341 tgcttgagtg tggcgctgat cgtggaatct gcccgcaggg cttgcgcgcc gaggatgatg
     2401 cagccaagcg tggtcgcgat gcggaggagt tctcgtttca tggcggagca gaatgaggct
     2461 tgcgattagc cgccgtttca ggtgtaatca ggaccattgg cggaattccc gctcattgaa
     2521 ccatcgccgc acgagctatg aaagccttta tttactccgc gtttggcctt tttatgactg
     2581 tcaccttctg ccccggccag ggcacgctga cacctccggg cccgcccggt gtcacgatga
     2641 aaacacttga tgaggtggag ccgcgcacgc cgatcaactc cctcacgacg ccgggggaca
     2701 gcggcgccgc cttcaaaatc acgcagcccg gctcctatgt gctcaccggc aacctcacgc
     2761 ccgccagcgg caaacacggc atccaggtca ccctcgccac ctcggggcgc gtggagatcg
     2821 acctgatggg cttcaccatc aacggtgcca gcgccggcgc gggcaaatcc ggcatcatga
     2881 tcgacgccgg cgcgggcaac gactccctgt gcagctacat gatcaaaaac ggcagcttgc
     2941 gcgccttcag cgcggcgaac atcaaggtcg atcccgcctg cctgggtgac acggccacgc
     3001 atgaggtggg ccattgggct ggactgacag cggtggagag cgccggacgc gtcaccatca
     3061 ccggaggtcg cattcaagac cccactggtt ctgcgatcat cgcgggcgcg gagtcgcgct
     3121 tcaccgggct ggagattcgc gacagcagca gcgggcctga tccgatcatc gtggctagcg
     3181 gcggcaattt ctccctcgac gatgtcctgg tctcctccgt ctccggtggc aattccaatc
     3241 aacccgccat cctgctcggc cccggctcca aaatccgcgg catgaccgcg actttttcag
     3301 gaggcaccta cacgggcacc attgtgggca ccaccagcgg cttcatggat gtgacaggac
     3361 tcgacatcgt catgaccggt gtcaccgcgg cctctggagt ttcctccctc caaacttcga
     3421 ttcagcggcc ttacgaattc tccaccacag gctccgccga tggcagcaca gttcagtggc
     3481 cgctggtgat ccgcgcggtg aacagcacct tttcctttgc catcgacgtg gtgcagtctg
     3541 gtctctccct cccgagttcc gaccactgcc cgcgcatcct tctggagggc accacgacga
     3601 cgcctgccgc cttctcgctc gaaagcagca atcactgccc cgcgatcatg gacatcggca
     3661 tcgccagcac agccagtgtc accggcgccg tcgtgcaggt caccggctcc ggtaacacca
     3721 tccgggccaa catccgcggc atccccggcg gaggcacggg cattcgtttc ttttccggct
     3781 ccggcaacac cgtggagagc agcaccttca tcggccgtgg cgacagcgcc atcaccgccg
     3841 tgcgcctcga caacggcgtc accaacacac tcgtgcgcaa caaccgaggc agccgctggt
     3901 tcggcggcag cgtcatggtc aacaacctgg gcgacaacac caacgccatc gccccggtgg
     3961 gcaattccac caacctcagc accaactcca acccgatgtc gaacttcgtg cagtgatggc
     4021 ggcaacgacc tgaatctggc attcactgca gcccgccttc atgcacgcct cgccggcctg
     4081 ggtgtgagct gctgatgacg atacctggct ggcgaaagcc caatggtgtg gcggaagcag
     4141 cgcttgaagt aagcgtaatc gtaaaagccg atctccacgg ccagcgtggc gatggtgtcc
     4201 tcagtctcgg ccgggcggcc taaatagaaa atgaaaagct gcctggcaaa tggacagcaa
     4261 ccacccctac ccatgccaca cacgcgcggc acaacgcgcc ttaattcggt gcaaatgagc
     4321 cattgattgt gaatgtgttg ccttaatgaa aactgctttt gcctggcaac tgcttttgga
     4381 ctttggagga tgctgaacgt tggcggataa aaagtcctgc atggaaatgc ttgtgtaacg
     4441 gtgttatgcc aagtgaactc gacttgacct tccgctgatt ttgaaaaaaa taaaaattat
     4501 tttctttggg ttgttcgcat ctgctttgtc agctttgctt gtcatcttgg ctgctgtatt
     4561 gatttttgag catataatcg gggataagga accaatcatt ggctatgcaa taacttgctt
     4621 gattgccctt ggtgtatttc ttcttggatg catgataacg tgtatttgta ccgcgcgctc
     4681 ttgaccgaat gacgcggcgg gcatccgcga cagcccttac cgcctggaat aggcaagtta
     4741 acatggcgca gcccgtttta tggcaacgcc tgcacttgca ggtgtgagat tgatagttcg
     4801 cgagcgatat gctaaaccga agcgtaatga gcagtgtcat gaaaatgaaa ccccttgttt
     4861 tttgctgctt ggctatgctc ctggcaattt tgccggcgca aggagagagc aagcacatga
     4921 tgaaaatcac aaccacaacc gaaggtgacc tctttgccga ttcagagcat gaagctcgca
     4981 ctcgcgaatg gacccctctg agtgactgga ttgttccgca aaataacttg gcgttgattg
     5041 cccaagcaag cctgcaggag aagcatggtt cgcgcatgaa agtctttaat gcagagctta
     5101 cccatgtatt ttggactcgt tctttcgagg gatggtggtt tcttcaaatt cagtttttga
     5161 ttcgttcgaa ggaatcatca ccttgcagcg gcgctgaaat gaaaagcatc tatctgttgc
     5221 ctgacggggg tcttttgcgg ctcgttcaga aataggaaaa agcggcagtt ctatctctcc
     5281 tcgtcgggtt ttgatcttgg cggctcaagg gtgccagggc agccggtaga tcgaaattcg
     5341 aaactcgaag gatgaggcgg ggagcagatg caggcagttc gatggaggtg tcgggtaata
     5401 gtggctcaaa ccattgctgg tttgcctcag tcattgctgg aatctcgcgt ttgaaagttg
     5461 ggttgcggct gaccaacggc tggctgcggc atccgaacga cggtattgac cccgcatccc
     5521 gccgggggca gcgggagaac catgcgccgg tacaggctcc caccagaaga attggggtga
     5581 cgcagcagct acgggcgcgc aacttgaggc cgccatcgcc gtgcgcgcgg ccttgaccgc
     5641 atggcgcgcg cgggcatcat cggcgtgcca tgaaaagcgc ggactcccaa ggactcggac
     5701 gggcggaaaa ggccatccag gtggtcatca tcctgtccct cgtcactttc gccctggaga
     5761 cgctgcccgg cctgctgccc tggcaaaggc gcgcgctggc cgcgtttgag atgttttcca
     5821 tcgcggtgtt cacgatcgag taccttgtgc gcctgtccct ggcgcgccct gtctcgaagt
     5881 atgccttcag cttcttcggc atcgtggact tgctggccat cctgccgttt tatctgagcg
     5941 cggggtttga cctgcgctcg ctgcgcgcct tccgcctgct gcggctgctg cgcgtgctga
     6001 agctggcgcg ctacagccgc gccatgcagc gctttcaccg cgcgttcatc atcgccaggg
     6061 aggagcttgt gctcttcggt gccacggcct gcatcctgct ctacctggcg gcggtgggca
     6121 tttactactt tgagaatgcc gcgcagccgc aggtcttcac ctcgctcttc gacgggcttt
     6181 ggtgggccat ctccacgctg accaccgtgg gttacggtga cgcgtatccg atcacggcgg
     6241 gcggcaggtt tttcaccttt ttcatcctta tcatcggcct cggcgtcatc gccgtgccca
     6301 cggggctgat cgcctccgcg ctctcgaagg cgcggcgcga ggaggacgag gaagaagcgc
     6361 gggcccttga agccgccgct atcgcacaaa cttccccgga ttcagaatcc ccttcgggtc
     6421 cagggcctgc ttgagccgca ggtgcagggc gcgcgcgccc tcggacacgg cgtgcgggaa
     6481 ccagcgctct ttggcgatgc ccacgccgtg ctcgccggtg atggccccct tccaggcgat
     6541 gacctggtgg aaaagcttgt cgagcgcggc ctcggcgcgc tcgcggatgg ccgggtcgtc
     6601 catggtgggg accatgatgt tgacgtggat gttgccatcc ccggcatggc cgaagcaggc
     6661 cacggggaag ccgaactcac cctgcaaggt ctcggcgaag tccacgaggt ccacgagccg
     6721 gctgcgcggc accacgatgt cctcgttgag cttgatgagg ccggtgttgc gcaggctgta
     6781 gctgaactcg cggcgcagct tccagaagcg ctcgcaggcc gcctctccca gcgcctcctc
     6841 caggcagatg cagccgagcc cgcgcacgag ctccgccagc gcggccagct cgcctttgac
     6901 cgaagcctcg tggccgtcga gttccacgag caggtgcgcg tcaccctgtg gtgtgacgga
     6961 ttcccccaga tactcgcgcg cggcgcgcag ggtgaatttg tcggcgatct caagagcgct
     7021 gggcagataa ccggaatcca aaatccggcc caccgcgttc gccgcctcgg cgaagctgcc
     7081 gaagcctgcg gagagcatgg cgcgcatggg aggatgcggg atgaggcgca gcgtggcctc
     7141 ggtgacgaca ccgagcaggc cctcgctgcc gaccatgagg cccacgaggt caaagccggt
     7201 cttgtttttg tggcagcgcc cgcccgcttt gaccacggtg ccgtcggcca gcaccgcctc
     7261 aaggcc
//
LOCUS       DAOMYJ010000248         7219 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Prosthecobacter sp. isolate AMR_MDS_5629
            NODE_3478_length_7219_cov_20.043551, whole genome shotgun sequence.
ACCESSION   DAOMYJ010000248 DAOMYJ010000000
VERSION     DAOMYJ010000248.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867850
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prosthecobacter sp. (wastewater metagenome)
  ORGANISM  Prosthecobacter sp.
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Verrucomicrobiaceae; Prosthecobacter.
REFERENCE   1  (bases 1 to 7219)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7219)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.30
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:34:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,742
            CDSs (total)                      :: 4,684
            Genes (coding)                    :: 4,676
            CDSs (with protein)               :: 4,676
            Genes (RNA)                       :: 58
            tRNAs                             :: 55
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7219
                     /organism="Prosthecobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3478_length_7219_cov_20.043551"
                     /isolate="AMR_MDS_5629"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1965333"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..3208)
                     /locus_tag="PLP58_19045"
     CDS             complement(<1..3208)
                     /locus_tag="PLP58_19045"
                     /inference="COORDINATES: protein motif:HMM:NF025295.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="immunoglobulin domain-containing protein"
                     /protein_id="HRJ10686.1"
                     /translation="MKTLHYSILCALGLGMFHATEARAAVIFGAPHINTTPGAVVADG
                     TTVTMNASVTTDSGLALYQWLKDGLPLANGGGVAGATTNKLVIKPAKNTHTGSYRVQV
                     REIILGGGVGDPEISDPFDLTVHQRPVITTHPLPQNKNQDDGLMLSVAVSPLSEPTLQ
                     YEWQLNNVPIDQMANPSAGTADFVIPARDPMDPVNVPGLQLPNAGNYRVKVSNLSGIS
                     VFSKTALVKINSAALILEPLPAQFFIATKATGKLAVKAGGTPKLLYQWEIEGEGNVAK
                     GGNAAAISIPGVAANDGKKYRVTVSNALNNATHPADVSSWVEIKVIDKVVAPEVTAAL
                     GINNFAKAATFDSGTAPVLTVQASMVNTGTLMYQWQKDGKNISDTATMTGTNSRDLVF
                     TDIRWQDRGVYRCIVKNEVSVVTSKTFKVEVNSHPVILTQPATNLVGITGGGATTSVI
                     VGGSGKPLFQWFKKTGGPPQPVNKATTNKLNLTKLAKTAPAGEDYYCEITNPFTATLG
                     GGMATTTNDCNLVVFDPAKITTHPASQSVRVNNNITMTVGVTGDTPMVFEWWFGKIKL
                     ANGPLGSAIISGADTASLTITNIQSGLQGSYYCMVRNAQLPGQPKYLVNAKSKTATIK
                     VIMPPTIARQTQASPASPVVEEDRVTVSIVGGGTPPLKYQWQMKVGVGGTWGDMKGKT
                     AAQLIFGKITLADDAFYRCVVNNSLNEPVTSDELELKVDLIPSPVITSFTPSIGRGGE
                     FIRVTGSNFNYAKTVSHMPALGNPVNTTFVIESDTSLLITVANAAPTTASPIRIANRG
                     SNVPTTSATNYTRTTLYQNDSSNPRIMIKATETRILGDNTGLDDPLFGRPLAWYSWTP
                     PLAGNYALVLDSTTVVYDPIIIFRRPAAGGGFTTTFWDTPVYSTQEIYQFSITAANVG
                     RQHFYAMGAFIGSGLPTEGPFVFDLLYSPSFEPPTSDDVNDSSAAAASLPMKSETSAN
                     SSFRLVENMDGEQLVQLGGNASALEPTVLWSETASNMSASNVVCSFHAGLDAVGEGLS
                     DVFAWQATGVDGAPLLQVRLNSADGSVELVQPDGTTQTA"
     gene            complement(3319..4911)
                     /locus_tag="PLP58_19050"
     CDS             complement(3319..4911)
                     /locus_tag="PLP58_19050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009960735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase (NADP(+))"
                     /protein_id="HRJ10687.1"
                     /translation="MLTGHHLIAGRETTTRHSPFQAVNPAANQLMEPHFSEATAAEAD
                     EALRAADGAFDALRTAPVGTRAAFLETLAEEIEALGDALLERAHAETALPMARLTGER
                     GRAVNQCKMFARLIREGSWAEAAIDRALPDRQPLPKPDVRRVLQPVGPVVVFGASNFP
                     FAIGVAGTDSVCALAAGCPVVVKAHPAHPGTCEMLSRAVLAALQKNGLPQGCFSMLHG
                     RGHEIGTELVRHPLTQAVAFTGSLRGGRALMDVAAARPHPIPVYAEMGSVNPVFVLPG
                     ALKERAAKMAEAYIGSVTMGVGQFCTNPAMVLGVKSAELGKFVKTAGELAAKVAPQTM
                     LHRGICETYEAGTAVWHTVSGIQLAGQSAAAPDHDATQAACRIFHTRLDVLQANEELQ
                     REVFGPCSIVAESDDMESILDFARGLEGQLTAAIHGTPDDLREHAALIRILERKAGRI
                     IFNGFGTGIEPCPSMHHGGPYPAASHSFFTSIGTGSIYRFVRPVCYQGFPDDCLPEAL
                     QNANPRGIMRLLDGSLSRAPAV"
     gene            complement(4954..5781)
                     /locus_tag="PLP58_19055"
     CDS             complement(4954..5781)
                     /locus_tag="PLP58_19055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009960468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HRJ10688.1"
                     /translation="MQLYRTRQGIYLHTASTWHRLDEEDWDALFNRADLHEHLAGVAA
                     QLTAVKGPGPEEILAPVGSQEVWAAGVTYFRSRTARMEESRDAGGGSFYDRVYEAERP
                     EIFFKATPQRVARPGQPMHLRSDSKWMVPEPELTLAINAEGRIIGFTAGNDLSCRDIE
                     GENPLYLPQAKCFKLCAALGPCLRVAPEPPPPETAIRLQILRAGAPAFEGETTLAQLK
                     RTPRELAGFLFRDNTFPAGAYLMTGTGIVPGDDFTLQSGDEMRITIDGIGTLVNPAA"
     gene            5924..>7219
                     /locus_tag="PLP58_19060"
     CDS             5924..>7219
                     /locus_tag="PLP58_19060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007413134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HRJ10689.1"
                     /translation="MKRLLLASLLLAPALPAVPPNIILIMSDDMGYSDLGCYGGEIET
                     PNLDALAAGGVRFTQFYNTARCCPTRAALLTGLYPHQAGIGHMMDDRGLDGYRGELSR
                     RAVTIAEALRPAGYRSYMAGKWHVTQKVRPEGDSDKNNWPLQRGFHRFYGTIHGAGSF
                     FDPNSLTRDNTLISPFADPEYQPAEFYYTDAINDHAARFVTEHARQHPDQPFFMYMAH
                     TAAHWPMHAKEKDTAKYKGRYDAGYDAIRAARVEKMKRLGLIDPRWQTAPQAGGAWAD
                     VKDREFELRCMEVYAAMVDCMDQGIGRLVRQLKDNGQYDNTLILFLQDNGGCAEGMGR
                     KVEGAAPRHRAASPSLPPLAADYLQPDMIPRQTRDGHPMRQGHGVLPGAADTYHGYGE
                     AWANVSNTPFREYKHWVHEGGISTPLIAHWPAGIPAARRG"
BASE COUNT         1351 a         2290 c         2265 g         1313 t
ORIGIN      
        1 cggcggtctg ggtggtgcca tccggctgca cgagttccac gcttccatcc gcgctgttga
       61 ggcgcacctg aagcagcggc gcaccatcaa ccccggtggc ctgccaggca aagacatcac
      121 tcaacccctc tccgaccgcg tccaatcccg cgtgaaagga gcacaccaca ttcgaagcgg
      181 acatattgga agcagtctcg ctccaaagaa cggtcggctc aagagccgaa gcatttccgc
      241 caagctgcac tagctgctct ccatccatgt tttcaaccag tctgaaagag ctattggctg
      301 atgtctccga cttcattggg agggaagctg cggcggctga cgagtcattg acatcatcag
      361 aagttggcgg ctcaaaagag ggactgtaga gaaggtcaaa aacaaagggg ccttctgtcg
      421 gcaatcctga gccaatgaag gcacccatgg cgtaaaaatg ctgccgaccg acgttcgcag
      481 cggtgatgct gaattggtag atttcctgtg tcgaatagac gggggtgtcc cagaatgtcg
      541 ttgtaaaacc gccgcctgcc gccgggcgcc ggaagataat gatcggatca taaaccacgg
      601 ttgtggaatc caggaccagt gcatagtttc cggcaagggg cggggtccaa ctgtaccaag
      661 caagaggcct cccaaacagc ggatcgtcca atcctgtgtt atcacctagt attcgtgttt
      721 ccgtagcctt gatcatgatg cgagggttag aggaatcgtt ctggtaaaga gtggtacgag
      781 tgtagttggt cgcggacgtg gtcggtacat ttgacccccg gttggcgatg cggataggtg
      841 atgccgttgt cggggccgca ttggccaccg taatcagaag cgaagtgtcg gattcgatga
      901 caaaagtggt gttcaccgga ttgccaagcg caggcatgtg cgagacggtc ttcgcatagt
      961 tgaaattgct gccggtcact cgaataaact cgcccccgcg cccgatggat ggcgtgaagc
     1021 tcgtaatgac aggagagggg atcaaatcca ctttgagttc caactcatcg ctggtgaccg
     1081 gctcattgag agagttgttg accacgcagc ggtagaaggc gtcatccgcc agggtgattt
     1141 tgccaaaaat caactgagcc gccgttttgc ctttcatatc gccccaggtg ccgccgaccc
     1201 ccaccttcat ctgccactga tatttcagcg gaggcgtgcc cccgcccaca atggatacag
     1261 tgacacgatc ctcctccact acaggcgagg caggtgaagc ctgggtttgc cgggcaatgg
     1321 tgggcggcat gataactttg atggtcgctg tcttggactt cgcgttgacg agatattttg
     1381 gctggcccgg aagctgggcg ttgcgcacca tgcagtaata actgccctgc aggccactct
     1441 gaatgttcgt gatcgtgagg ctggcggtgt ccgcgccgga aatgatggcg gagccgagcg
     1501 gaccgttggc cagcttgatt ttgccaaacc accattcgaa aaccatgggg gtgtcccccg
     1561 tgacacccac ggtcatggta atgttgttgt tcacacgcac cgactgcgat gccggatggg
     1621 tggtgatctt ggcgggatcg aagaccacaa gattgcagtc atttgtcgtg gttgccattc
     1681 cgccgccaag cgtggcggta aatgggttgg tgatctcaca ataataatcc tcacccgctg
     1741 gagcagtttt ggccagtttg gtcaggttca gcttgttggt ggtggctttg ttgacaggct
     1801 gtggaggtcc accggtcttt ttaaaccatt ggaaaagggg tttgccgcta ccgccaacaa
     1861 tcacagatgt cgtggcaccg cccccagtga tgccgaccaa gtttgtcgcg ggctgagtga
     1921 gaatgaccgg atgagaattg acctccactt tgaaagtctt ggaggtgaca acgctgactt
     1981 cgtttttcac aatgcagcgg tacacaccgc gatcctgcca gcgaatgtcc gtaaagacca
     2041 gatcccgaga gttggtgccg gtcatcgtgg cggtgtcgga gatgtttttg ccgtccttct
     2101 gccattggta catcagagtg ccggtattga ccatgctggc ttggactgtg aggactggcg
     2161 ctgtccctga gtcaaatgtc gcagctttgg cgaaattgtt gataccaagc gcggcggtga
     2221 cctccggggc gacaaccttg tctatgacct tgatctccac ccaactgctg acatccgccg
     2281 gatgtgtcgc gttgttgagc gcgttggaga cggtgacgcg gtatttttta ccgtcattgg
     2341 ccgccactcc ggggatggaa atggccgccg cattgccgcc cttggccaca ttgccctcgc
     2401 cttcgatctc ccactggtag agcagcttgg gggttccccc ggccttcacg gccagtttcc
     2461 cggtggcctt cgtggcgatg aaaaactgcg ctggcagcgg ctccaggatg agcgcggcag
     2521 agttgatttt gacaagcgcg gtcttgctga aaacggagat gccggaaagg ttgctgacct
     2581 tgacccggta gttgccggca ttcggaagct gcaatccggg cacattgaca ggatccatcg
     2641 ggtcacgcgc gggaatgaca aaatccgccg tgccagccga tgggttggcc atctggtcaa
     2701 tgggcacatt gttcaactgc cactcatatt gcagagtcgg ctcgctgagc ggactgacag
     2761 ccacggaaag catcaagccg tcatcctgat ttttgttctg cggcagggga tgcgtggtga
     2821 tgaccggtct ctgatgaaca gtcagatcaa acggatcgct aatttcagga tcccccacgc
     2881 ccccgccgag aatgatctcg cgcacctgaa cccggtagga gccggtatgc gtgtttttcg
     2941 cgggcttgat gaccagtttg ttggtggtgg cacctgcgac acctccgcca tttgcaagcg
     3001 gcagaccgtc cttaagccac tgatagagag caaggccgct gtccgtggtc acggaagcgt
     3061 tcattgtgac cgttgtgcca tcagccacca cggcgcccgg tgtcgtgttg atgtgcgggg
     3121 cgccaaagat caccgccgct ctggcctcgg tcgcgtgaaa catgcccagt ccgagggcgc
     3181 aaagaatcga ataatggagg gttttcataa actagctggg gggttaagtc cgattttctc
     3241 tgactttctg cctcgtataa acggatagcg catacaatga caatacaaaa attcagaaga
     3301 atccgggaac cgccgaattc acaccgccgg ggcacggctc aatgagccgt ccagcaggcg
     3361 catgatgccg cgcgggttgg cgttttgcag cgcctcaggc aggcagtcgt ccgggaagcc
     3421 ctggtagcag acggggcgga caaagcggta aatgctgccg gtgccgatgc tggtgaaaaa
     3481 gctgtggctg gcggcgggat agggtccgcc gtgatgcatg gaggggcagg gttcgatgcc
     3541 ggtgccgaag ccgttgaaaa tgatgcggcc cgctttgcgc tccaggatgc ggatgagcgc
     3601 ggcgtgctcc cgcaggtcgt cgggcgtgcc gtggatggcg gcggtgagct ggccttccag
     3661 tccgcgcgcg aagtcgagga tggactccat gtcatcactc tcggcgacga tggagcacgg
     3721 gccgaagacc tcgcgctgaa gttcctcgtt ggcctgcagc acatccaggc gggtgtggaa
     3781 aatgcggcag gcggcctggg tggcgtcgtg gtcgggcgcg gcggcggact ggccggcgag
     3841 ttgaatgccc gaaacggtgt gccagacggc ggtgccggcc tcgtaggtct cgcagatgcc
     3901 gcggtggagc atggtctgcg gggcgacttt ggcggccagt tctccggcgg ttttgacgaa
     3961 cttgcccaat tccgcgcttt tcacgccgag gaccatcgcg ggattggtgc agaactgccc
     4021 cacgcccatc gtcaccgagc cgatgtaggc ctcggccatt ttcgcggcgc gctccttgag
     4081 cgcgccgggc agcacaaaga cggggttcac cgagcccatc tctgcataga ccgggatggg
     4141 atgcgggcgc gccgcggcca catccatcag cgcgcgtccg ccgcgcaggc tgccggtgaa
     4201 ggccaccgcc tgggtgagcg ggtgcctcac cagttccgtg ccgatctcat gcccgcgccc
     4261 gtgcagcatg gagaagcagc cctgcggcag gccgtttttt tgcagcgcgg ccaggacagc
     4321 gcgggaaagc atctcgcagg tgccggggtg cgcggggtgc gccttcacca ccaccgggca
     4381 gcccgccgcc agggcgcaca ccgagtcggt gcccgccacg ccgatggcga aggggaagtt
     4441 gctggcgcca aagaccacca ccgggccgac gggctggagc acgcggcgca cgtccggctt
     4501 cggcagcggc tggcggtcgg gcagagctcg gtcaatcgca gcctcggccc acgatccctc
     4561 gcggatgagc cgggcgaaca ttttgcactg gttcaccgcg cggccgcgct cgccggtcag
     4621 gcgcgccatc ggcagcgcgg tctcggcatg ggcgcgctcc agcagggcgt ctccgagggc
     4681 ctcgatctcc tccgccagag tctccaaaaa cgccgcccgt gtcccgacag gagcggtgcg
     4741 cagcgcgtcg aaggcgccgt ccgcagcgcg cagcgcctca tccgcctcgg cggcggtggc
     4801 ctcggagaaa tgcggctcca tgagctggtt ggccgccgga ttcacggctt gaaaaggcga
     4861 gtgccgggtg gtggtctcgc gacccgcgat gaggtggtgt ccagtgagca tgatgggaaa
     4921 aagaaaaggg ggagaggaaa gctgccgcgc ggctcaggcc gccgggttca ccagcgtgcc
     4981 gatgccgtca atggtgatgc gcatctcatc gccggactgg agggtgaaat catcccccgg
     5041 cacgatgccg gtgccggtca tgaggtaggc gcccgcgggg aaagtgttgt cgcggaacaa
     5101 aaagccagcc agttcacgcg gggtgcgctt gagctgggcc agcgtcgtct ccccctcgaa
     5161 ggcgggcgcg ccggcgcgca ggatttgcag gcggatggcc gtctcaggcg gcggcggctc
     5221 cggcgccacc cgcaggcagg ggccgagggc ggcgcagagc ttgaagcact tcgcctgcgg
     5281 caggtagagc gggttttcac cctcgatgtc ccggcaggag aggtcattgc ccgcggtgaa
     5341 gccgatgatc cggccctccg cgttgatggc cagcgtcagc tccggctccg gcaccatcca
     5401 tttcgagtct gagcgcaggt gcatgggctg gccggggcgc gccacgcgct gcggggtggc
     5461 cttgaaaaaa atctccggcc tctccgcctc gtacacccgg tcgtaaaagc tgcccccgcc
     5521 cgcgtcccgg ctctcctcca tgcgcgccgt gcggctgcgg aaatacgtca cccccgccgc
     5581 ccacacctcc tggctgccga cgggcgccag gatttcctcc ggccccggcc ctttcacggc
     5641 ggtgagctgc gccgccacgc ccgccagatg ctcgtgcaaa tccgcgcggt tgaagagcgc
     5701 gtcccagtcc tcctcatcca gccggtgcca ggtgctggcc gtgtggagat agatgccttg
     5761 gcgggtgcgg tagagctgca tgagggcgcg agactatcgg gtgcggcgcg gctgtcaaaa
     5821 caagatgcgc gtgtgcggaa agaataggcc ctggtgcgga aagacatgat gacttgccgc
     5881 cgcgcccggc gttgtcaccg ccgccccatt ccatgcccag accatgaaac gactcctgct
     5941 cgcctccctg ctcctggccc cggccctccc cgccgtcccg ccgaacatca tcctcatcat
     6001 gtcagacgac atgggctact ccgacctcgg ctgctacggc ggcgagatcg agaccccgaa
     6061 cctcgacgcc ctcgccgccg gcggagtccg cttcacccag ttttacaaca ccgcccgctg
     6121 ctgccccacc cgcgccgccc tgctcaccgg cctctacccg caccaggccg gcatcggcca
     6181 catgatggac gaccgcggcc tcgatggcta ccggggcgag ctcagccgcc gcgccgtcac
     6241 catcgccgag gccctccgcc ccgcaggcta ccgctcctac atggccggca aatggcacgt
     6301 cacccaaaaa gtgaggcccg agggcgactc cgacaaaaac aactggccgc tccagcgcgg
     6361 cttccaccgc ttttacggca ccatccacgg cgcgggcagc ttctttgatc cgaactccct
     6421 cacccgcgac aacacgctca tctccccctt tgccgaccct gaataccagc ccgccgagtt
     6481 ttactacacc gacgccatca acgaccacgc cgcccgcttc gtcaccgagc acgcccgcca
     6541 gcacccggac cagccctttt tcatgtacat ggcccacacc gccgcccact ggcccatgca
     6601 cgcgaaggaa aaagacaccg ccaaatacaa aggccgctat gacgccggct acgacgccat
     6661 ccgcgccgcg cgggtggaaa aaatgaagcg cctcggcctc attgaccccc gctggcagac
     6721 cgccccccag gccggcggcg cgtgggcgga cgtgaaggac cgcgagttcg aactgcgctg
     6781 catggaggtc tatgccgcca tggtggactg catggaccag ggcatcggcc gcctcgtccg
     6841 gcagctcaaa gacaacggcc agtatgacaa caccctcatc ctcttcctcc aggacaacgg
     6901 cggctgcgcc gaaggcatgg gccgcaaagt cgaaggcgcc gccccccgcc accgcgccgc
     6961 ctccccttcc ctgcccccgc tcgcggcgga ctacctccag cccgacatga tccccaggca
     7021 gacccgcgac ggccacccca tgcgccaggg ccacggcgtc ctccccggcg ccgccgacac
     7081 ctaccacggc tacggcgagg cctgggccaa cgtcagcaac acccccttcc gcgaatacaa
     7141 gcactgggtc cacgaaggcg gcatcagcac cccgctcatc gcccactggc ccgccggcat
     7201 ccccgccgcc cgccgcggc
//
LOCUS       DAOMYJ010000249         7177 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Prosthecobacter sp. isolate AMR_MDS_5629
            NODE_3506_length_7177_cov_19.287981, whole genome shotgun sequence.
ACCESSION   DAOMYJ010000249 DAOMYJ010000000
VERSION     DAOMYJ010000249.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867850
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prosthecobacter sp. (wastewater metagenome)
  ORGANISM  Prosthecobacter sp.
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Verrucomicrobiaceae; Prosthecobacter.
REFERENCE   1  (bases 1 to 7177)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7177)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.30
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:34:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,742
            CDSs (total)                      :: 4,684
            Genes (coding)                    :: 4,676
            CDSs (with protein)               :: 4,676
            Genes (RNA)                       :: 58
            tRNAs                             :: 55
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7177
                     /organism="Prosthecobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3506_length_7177_cov_19.287981"
                     /isolate="AMR_MDS_5629"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1965333"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(44..1297)
                     /locus_tag="PLP58_19065"
     CDS             complement(44..1297)
                     /locus_tag="PLP58_19065"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HRJ10690.1"
                     /translation="MSITPDTLRPETLAELEANLEQMKTANYFLRVAVDQVPEAVLIV
                     EAESGSPHGPRVLFSNAAAAVLVGVEPEKGLRGMNLADLAAGDMDAAVLMQSLRESVT
                     NGGSHECECRVQNLYGREPLMCRWRARAVFNSQRKLLNHTLMITPKPALSAAAARTAP
                     LNGGNLDAQSELLKQANLALLARGIAHDVNNLLGIPFMALSEMLQDAALPPGSRDTLT
                     QIYQSLDLARQFTSRVLSACKDQPGTVKPADIAAIIQSTVKVAGAGANVELRVRAAGD
                     LHRPVIDGVRLAQVLQNLILNGIQAMPTGGYMDIEAANTEFAEGQDEQLKAGRYVRII
                     VRDRGCGIPPENLARLFREPFTTKPGGNGIGLTTCRHYIKSEQGDIRVSSRTGVGTEF
                     TVYLPVRDTPPQTPPDDTAAAPPPC"
     gene            complement(1456..2514)
                     /locus_tag="PLP58_19070"
     CDS             complement(1456..2514)
                     /locus_tag="PLP58_19070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007414672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HRJ10691.1"
                     /translation="MNRRAFLRQTALASTALAGGRLRAAVPGLKLGLDHFALRATGWK
                     AAQYIDYAASQKLDTCFISEMHIFESFEDAYLQGLKDQADRAGLRLYVGTGSVCPTSN
                     TWKDTYGTPEEHLALTLRVAKKLGSPVARCYLGNSKDRATDGGIQRHIEQTVKVIRAS
                     RALAETEEVKVAIENHSGDMQSHELKALIEAAGKEYVGANLDPGNAVWALEEPLAHLE
                     VLGALTVCTSARDSMVWENEDGAMVQWTAVGEGLVDFKAYAKRFSELAPGVPLQIETI
                     SGFARPFAWKKDEFWAPYPNHKDSAAFAAWVGMSKKGREIPSFKAPDGPGKKDAEIAY
                     QKSEFERSIAWLNSNCRS"
     gene            complement(2798..3643)
                     /gene="ispE"
                     /locus_tag="PLP58_19075"
     CDS             complement(2798..3643)
                     /gene="ispE"
                     /locus_tag="PLP58_19075"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009961205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050515 - 4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HRJ10692.1"
                     /translation="MKLKLSSPAKINLWLRILGRRQDGFHEVETRLHKVAVEDVVELE
                     LTGPGEDVTLTCTVPGIPLDDSNLAIRALRAFEARAGSRHSWRIHLEKRIPSGAGLGG
                     GSGNAAVILQGANQLLGSPLGIDELLEVAAGIGADVPFFLLETSAADGSGRGEQVAPA
                     PSPGPVPVLLIKPAFPVPTPWAYGRWMGSRELPGVMYAPQPGPWGALQNSLERPVFEK
                     FRLLPALKTWLLERPGVLGALMSGSGSTLFALFESSDACETAAGEAREWVGETAWIRA
                     TNLLG"
     gene            complement(3640..4329)
                     /locus_tag="PLP58_19080"
     CDS             complement(3640..4329)
                     /locus_tag="PLP58_19080"
                     /inference="COORDINATES: protein motif:HMM:NF014619.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyphenol oxidase family protein"
                     /protein_id="HRJ10693.1"
                     /translation="MDAINPNELCMRFAVLDGLGDFRHSFILRHPEISVDAERDEFLE
                     RLGPWHREQVAGLGFDPVALKLVAQVHGNGVAVIEEGNSESFFTGQDGMVSDRAGIML
                     GIYVADCAAVYLADPVGGAFGLVHSGKKGTESGIAAVALRLMMERFGTRPGNVLAQVS
                     PCIRPPAYEVDFAAEIRRQCLGCGVLPEHFHDCGVCTSSDLRRYYSYRMEKGRTGRML
                     ALLGKPGPAAS"
     gene            complement(4380..4829)
                     /locus_tag="PLP58_19085"
     CDS             complement(4380..4829)
                     /locus_tag="PLP58_19085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009961459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HRJ10694.1"
                     /translation="MALDTSSEIKDHAKLADFILFSQREFLLNLSRELNKDNISFAQF
                     FLLSYLASSKELTMTDIARKMSHSTAAATGLVDRLEKLGYMERTHAIDDRRKVMVRVT
                     AKGLNLVSRLREVLKGQIAEAMNEADAQDAATFMTNYASLDSAAMAR"
     gene            5014..5757
                     /locus_tag="PLP58_19090"
     CDS             5014..5757
                     /locus_tag="PLP58_19090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ10695.1"
                     /translation="MRFSKIFNNERSFVKNVGVRTLFQTATDGSITGSGVVLHNLPCQ
                     HLSMFLAEIDPVQIVIIVIAMAAGFVQWLWGLFQQNKAERQRGMQPAEADEEIEKLRE
                     EAWRKQVAGESAPSKPSPQAPRPVSDDNPWTVIREIMEKAQEATKQATPPPLPPPVQT
                     RPPALERQPAAPRSAKEAPPPPRTPALPAAPIPPSRAFPVTSRARSQGSAALFAKQLS
                     RPESIRQAILMREILGPPKALQSPADAPL"
     gene            5773..5856
                     /locus_tag="PLP58_19095"
     tRNA            5773..5856
                     /locus_tag="PLP58_19095"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5807..5809,aa:Leu,seq:caa)
     gene            complement(6041..>7177)
                     /gene="mnmE"
                     /locus_tag="PLP58_19100"
     CDS             complement(6041..>7177)
                     /gene="mnmE"
                     /locus_tag="PLP58_19100"
                     /inference="COORDINATES: protein motif:HMM:TIGR00450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HRJ10696.1"
                     /translation="HGPASFTGEDLVEFHGHGGMLVTQMTLEALLAAGARAAGPGEFT
                     QRAYLNGKMDLTQAEAVMDLIHAHSTLALRAANEQLGGAIGREAAALKEELLPVLAHV
                     EAFIDFPDEDITPDTGAALAARMQSVVSRARGLVATSEQGRILRQGARTVICGEPNVG
                     KSSLLNLLSGFERAIVSPAAGTTRDTIEEFIQVHGIPLRLVDTAGLREGGDDVERTGM
                     DRARRELERADLVLEVVDGSRPPERRVEMPAALQGRRLLVLNKADLGISEAWQDSADA
                     IPLSCATGEGVEALREAVRSVIMRSGPMQADHPVAVNARHKECFARVAVRTEAALAAL
                     RADAAPEFVALDLREALQALGEVVGAVDVEQVLDIIFSSFCIGK"
BASE COUNT         1373 a         2173 c         2251 g         1380 t
ORIGIN      
        1 gcggcgctcg ggctggcgat cacctacggc gtcatgggcg tgatcaacat ggcggggggg
       61 cggcggcggt gtcgtccggc ggggtctgcg gcggggtgtc gcggacgggg agatagacgg
      121 tgaattcggt gccgacgccg gtgcgggagc tgacgcggat gtcgccctgc tcggatttga
      181 tgtaatgcct gcaggtggtc aggccgatgc cgttgccgcc gggcttggtg gtgaagggtt
      241 cgcggaacag cctggccagg ttttccggcg ggatgccgca gccgcggtcg cggacgatga
      301 ttctgacgta gcgcccggct ttgagctgct cgtcctggcc ttcggcgaat tcggtgttgg
      361 cggcctcgat gtccatgtaa ccgccggtgg gcatggcctg gatgccgttg aggatgaggt
      421 tttgcagcac ctgggcgagc cggacgccgt ctatgaccgg gcggtggaga tccccggcgg
      481 cgcggacgcg cagctcgacg ttggcgccgg cgccggcgac tttgaccgtg gactggatga
      541 tggcggcgat gtcggcgggt ttgacggtgc cgggctggtc cttgcaggcg ctgagcaccc
      601 gggaggtgaa ctgccgggcg aggtcgaggc tttggtagat ttgcgtcagg gtgtccctgc
      661 tcccgggcgg gagggcggcg tcctggagca tttcggagag tgccatgaag gggatgccga
      721 gcaggttgtt cacgtcatgg gcgatgccgc gcgcgagcag ggcgaggttg gcctgcttga
      781 gcagctcgct ctgggcgtcg agattgccgc cgttgagggg ggcggtcctg gccgcggcgg
      841 cggatagcgc gggcttgggc gtgatcatga gcgtgtggtt gaggagcttg cgctggctgt
      901 tgaagacggc gcgcgcgcgc cagcggcaca tgaggggctc ccgcccgtag aggttttgca
      961 cccggcattc gcattcgtgg gagccgccgt tggtgacgga ctcgcgcagg ctttgcatga
     1021 gcacggcggc gtccatgtca ccggcggcga ggtcggcgag gttcatgccg cgcaagcctt
     1081 tttccggctc gactccgacc aggacggcgg cggcggcatt gctgaagagc acgcgcggcc
     1141 cgtgggggga gccggattcg gcttcgacga tcaaaacggc ctccggcacc tggtccaccg
     1201 ccacccgaag gaagtagttg gcggttttca tctgttcgag gttggcttcc aattcggcca
     1261 gggtctcggg gcggagggta tcgggagtga tggacatggc atcaatggtt gatgcctttc
     1321 tatttagcca ccttaaatag tccggtaaag tttaattttt gggagaatgc tcctttttgt
     1381 ttctcatttt tgaaactaca aggatgtttt ctgatttcga acgttgtttg cggtgatttt
     1441 gaaaaatcag catcttcaac tcctgcaatt cgagttgagc caggcgatgc tgcgctcgaa
     1501 ctctgatttc tgataagcaa tctcggcgtc ttttttgccg gggccgtctg gggctttgaa
     1561 ggaggggatt tcccgccctt ttttcgacat cccgacccag gcggcaaagg cggcgctgtc
     1621 cttgtggttg ggatagggcg cccagaattc gtcttttttc caggcaaacg ggcgggcgaa
     1681 gccggagatg gtctcgatct ggagcggcac gcctggagcg agttcggaga agcgcttggc
     1741 gtaggctttg aaatccacca gcccctcgcc gacggcggtc cactggacca tggctccatc
     1801 ctcgttttcc cagaccatgc tgtcgcgcgc gctggtgcag acggtgaggg cgccgagcac
     1861 ttcgagatgg gccaggggct cctccagcgc ccagacggcg tttccggggt cgagattggc
     1921 gccgacgtat tccttgccgg cggcttcgat gagggccttc aactcgtggg actgcatgtc
     1981 gccggagtgg ttttcaatgg cgactttcac ctcctcggtc tcggcgagag cgcggctggc
     2041 gcggatgact ttcacggtct gctcgatgtg gcgctggatg ccgccatcgg tggcgcggtc
     2101 cttggagttg ccaaggtagc agcgcgccac gggtgatccg agttttttgg ccacccggag
     2161 ggtcagggcc aggtgctcct cgggcgtgcc gtaggtgtcc ttccaggtgt tcgaggtggg
     2221 gcagacgctg cctgtaccga cgtaaagacg caggcccgcc ctgtcggcct ggtctttgag
     2281 accctggagg taggcgtcct cgaagctctc aaagatgtgc atctcggaga tgaagcaggt
     2341 gtcgagcttc tgggaggcgg cataatcaat gtattgcgcg gccttccagc cggtggcccg
     2401 gagcgcaaag tggtccaggc cgagcttcaa acccggcacg gcggcgcgaa ggcggccacc
     2461 ggcgagcgcg gtggatgcaa gggcggtctg gcggaggaaa gcgcggcggt tcatttcggc
     2521 ggattcaaaa gacatttccg gggcggtcaa gcgaagtcct gccgtattgc cggggcggca
     2581 aacctcccgt ttcaaaaaaa ttatcaaaat tttttcaaca tcctcaaagg cagggaatca
     2641 aagctatcca cagtgatggc gggagaagcc tcccccggat ctggtcttga gagtgattgg
     2701 ttgttgtcat cacccctgcg gcaacgcggg acagtgttga ggttgatgca aacaaaggag
     2761 tcgggcggaa tcgggagccg cccggctcca acttttttca gccaagcaga tttgtcgccc
     2821 tgatccaggc ggtttcccca acccactcgc gcgcctcccc ggcagccgtt tcgcatgcat
     2881 cggaactctc aaacagggcg aagagagtcg agccggagcc ggacatgagc gcgcccagca
     2941 cccccggacg ctccagcagc caggttttca aagcaggaag caggcggaat ttttcaaaca
     3001 caggccgctc gaggctgttt tgcaaagctc cccacggtcc cggctggggc gcatacatga
     3061 cgccgggcag ctcccgcgat cccatccagc ggccataggc ccagggagta ggaaccggga
     3121 aggccggttt gatcaaaaga acgggcacag gccccggtga tggcgcgggc gccacctgct
     3181 caccacgtcc gctgccgtcg gcggcgctgg tttccagcag gaaaaacggc acatccgcgc
     3241 cgattccggc cgccacttcc agaagttcgt caatgcccag cggactgccg agaagctggt
     3301 tggcgccctg gagaatgacc gccgcattgc cgctgccacc tccgagtccg gcccctgacg
     3361 ggatgcgctt ttccaaatga atgcgccagg aatggcgact gccggcccgg gcctcgaagg
     3421 cgcgcagggc gcggatcgcc aggttggagt cgtccagtgg tatgcccgga accgtgcagg
     3481 tgagtgtcac atcctctccc ggcccggtca gttcgagttc gacgacatcc tcaacggcaa
     3541 ccttgtgcag gcgggtttcc acctcatgaa aaccgtcctg cctgcggccc aggatgcgca
     3601 gccagagatt gattttggca ggggatgaca gtttgagctt caagaggcgg cgggaccggg
     3661 tttgccgagc aaggccagca tccggcctgt gcggcctttt tccatgcggt aggaataata
     3721 gcgccgcaag tcggaagacg tgcagacacc gcagtcatga aaatgctccg gcagcactcc
     3781 gcagcccagg cattgacggc ggatttcggc ggcgaaatcc acctcgtatg caggcggacg
     3841 gatgcacggg ctaacctgcg ccagcacatt cccagggcgc gttccaaaac gctccatcat
     3901 gagtcgcagc gccaccgccg cgatacccga ctccgtgcct ttttttccag agtggaccaa
     3961 gccgaaagcc cctccaaccg gatcggcaag atacaccgcc gcgcagtccg ccacataaat
     4021 ccccagcata atgcccgccc ggtcgctgac catgccatcc tggcccgtga aaaaggactc
     4081 actgttccct tcctctatca cagcgactcc gttgccatgc acctgcgcga ccagcttcag
     4141 cgccacagga tcaaaaccca gtcccgccac ctgctcccga tgccaggggc cgagcctttc
     4201 caggaactcg tcgcgctccg catccacgct gatctccggg tgccgcagaa tgaatgagtg
     4261 ccggaaatct ccaagcccat caagaacggc gaacctcatg caaagctcgt ttgggttgat
     4321 ggcgtccatc caaaggcaaa ttctcccgtg ccaggagagc ggatcacatc aagacactat
     4381 cagcgggcca tggccgctga gtcgaggctg gcataattgg tcatgaaagt cgcggcgtcc
     4441 tgcgcgtcgg cctcgttcat ggcttcggcg atctggccct tgagcacttc gcgcaggcgg
     4501 ctcacaagat tgagaccctt ggcggtaacc cggaccatga ctttgcggcg gtcatcaatc
     4561 gcatgggtgc gctccatgta gccaagcttc tcaagacgat ccaccaatcc agtggcggcc
     4621 gcagtggagt gggacatttt acgcgcgatg tcagtcatcg tcagttcctt cgaactagcc
     4681 aggtaggaaa gaaggaagaa ctgcgcgaag gagatgttgt ccttgttcag ctcgcgggaa
     4741 agattcaaaa gaaattcacg ctgactgaac agaatgaagt ctgccagctt ggcgtgatct
     4801 ttgatttcgg aggaagtgtc gagagccatg ttcgaaggga aattatggtt aatgtgagaa
     4861 aatcctgata atttcaagat actttatcta tgtttgatga ttcatgcaag ttgaattatg
     4921 gaaatcataa accaaagacg ccatgctggt gaaattctca agaataagaa attaacagcg
     4981 tcgaattatc aactttgaaa agccttctga caagtgcgtt tttcaaaaat cttcaacaat
     5041 gaacgatcat ttgttaaaaa tgtgggcgtg cgcacactat ttcagaccgc gactgatggc
     5101 agcattacag gatcgggcgt tgtgctccac aatcttccat gccaacatct atccatgttc
     5161 ctagcagaga ttgatccagt tcagatcgtc atcatcgtca tcgccatggc agcgggcttt
     5221 gtgcaatggc tgtggggact gtttcagcag aacaaagcgg agcgtcagcg cggcatgcag
     5281 ccagcggagg ctgatgaaga aatcgaaaaa ctcagggagg aagcgtggcg caagcaggtg
     5341 gcaggcgaaa gtgcgccctc aaaaccttcc ccccaggccc cccgccctgt gagtgacgac
     5401 aatccatgga cggtcatccg tgaaatcatg gaaaaggcgc aggaagcgac gaaacaagcc
     5461 acgccccctc ctctgccgcc ccccgtacag acacggccgc ccgccctaga gcgccagccg
     5521 gccgcccccc gttctgcaaa agaggctcct ccgccgcccc ggacgcctgc gctacccgcc
     5581 gcgcccattc ctccgagccg ggcttttccc gtgacttcgc gagcgcgcag ccagggcagc
     5641 gccgccctct ttgcaaaaca gctttcccga ccagagtcca tccggcaggc catcctgatg
     5701 cgggaaatac tcggaccgcc aaaagccttg caatcaccgg cggatgctcc actctagccg
     5761 cccgcaacac ccgccggtgt ggtgaaattg gtagacgcgc tagactcaaa atcttgtgtc
     5821 cgtgaggacg tgtcggttcg agtccgacca ccggcacttt catgcgaatg cgttccattc
     5881 tgccatgtcg aaacttttca aactttcagg cggaaaaccg ccaacccgcg gggactgacc
     5941 cggcattgat tcatacctgg aacgcatctg ccgccgcgcg gcaacaacgt ccgcccaagt
     6001 tgtagctgtc accgccgaag cagcaggcgg aacggcgcga tcatttgccg atgcagaagg
     6061 aggagaaaat gatgtcgagc acctgctcga catccacggc gcccacaacc tctcccagag
     6121 cctgcaaagc ctcgcgcaag tccagcgcca caaactccgg cgccgcatcc gccctcaatg
     6181 cggcgagcgc ggcctccgtg cgaactgcca cacgggcaaa acattccttg tgccgcgcat
     6241 taacggccac tgggtggtcc gcttgcattg gtcctgagcg catgatcaca gatcgcaccg
     6301 cctcgcgcag ggcctcgaca ccctccccgg tggcgcagga caggggaatg gcgtccgcgc
     6361 tgtcctgcca ggcttccgag attccaaggt ccgccttgtt caaaacaagc aggcgccgtc
     6421 cttggagagc ggcgggcatt tccacacgcc gttcgggcgg acggctgcca tccaccacct
     6481 ccagcaccag gtccgcgcgc tccagttcgc gccgcgcgcg atccatgccg gtgcgctcga
     6541 catcatcccc tccctcacgc aatccggcgg tgtccaccag ccgcagcggg atgccatgca
     6601 cctggatgaa ctcctcgatg gtgtccctcg tggtgcccgc cgccggactg acgatggcgc
     6661 gctcgaagcc ggagagcagg ttcagcaggc tggatttgcc cacatttggc tcgccgcaga
     6721 tgaccgtgcg cgcgccttgc ctgaggatgc ggccctgctc gctggtggcc acgagaccgc
     6781 gcgcccgcga gacgacagac tgcatcctcg cggccagggc ggcgcctgtg tccggtgtga
     6841 tgtcctcgtc agggaagtca atgaacgcct ccacatgggc cagcactggc agcagttcct
     6901 ccttcaaagc agcagcctcc ctgccaatgg cgccgccaag ctgctcgttg gcggcgcgca
     6961 acgccagggt gctgtgcgcg tggatcaaat ccatcaccgc ctcggcctgg gtcaggtcca
     7021 tcttgccgtt caaatatgcg cgctgggtga actcgcccgg ccctgcggcg cgcgcgcccg
     7081 cggcaagcag cgcctccagt gtcatctgcg tcacgagcat gcctccgtga ccgtgaaact
     7141 ccaccaaatc ttctccagtg aagctggccg gcccgtg
//
LOCUS       DAOMYJ010000250         7151 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Prosthecobacter sp. isolate AMR_MDS_5629
            NODE_3521_length_7151_cov_23.005073, whole genome shotgun sequence.
ACCESSION   DAOMYJ010000250 DAOMYJ010000000
VERSION     DAOMYJ010000250.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867850
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prosthecobacter sp. (wastewater metagenome)
  ORGANISM  Prosthecobacter sp.
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Verrucomicrobiaceae; Prosthecobacter.
REFERENCE   1  (bases 1 to 7151)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7151)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.30
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:34:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,742
            CDSs (total)                      :: 4,684
            Genes (coding)                    :: 4,676
            CDSs (with protein)               :: 4,676
            Genes (RNA)                       :: 58
            tRNAs                             :: 55
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7151
                     /organism="Prosthecobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3521_length_7151_cov_23.005073"
                     /isolate="AMR_MDS_5629"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1965333"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..192
                     /locus_tag="PLP58_19105"
     CDS             <1..192
                     /locus_tag="PLP58_19105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ10697.1"
                     /translation="AATAILAMAEHQRNHPAEARAALAAAKTALAANWPAGTEANWYA
                     WLIADLLAREAGTLVGKSR"
     gene            216..1139
                     /locus_tag="PLP58_19110"
     CDS             216..1139
                     /locus_tag="PLP58_19110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ10698.1"
                     /translation="MSIERNPPMPPPPPPQRPSGPTKPPAPLKPEEAGERIGPCKLLQ
                     QIGEGGTGGREAGEMKRAVRGNVDSLHRMDEGFLYQPAPNPRGGFTCRAVKGEQITEA
                     ELDAAVAAETGLPPEQCAQVLKAYLRQMLAKGATSRWSPGLYGLIGFYPTAGGNQPGH
                     DDFHTAEQVKAGMSIAFSLDAIRAWRQGLRLVSQGFTGRVTPKLATVICCTTDKPDVY
                     VSGHLIGVRGAHLKLDQHDPAQGIFFILADGTEVRCETYGGNTRLRVYGRVPEGVTGP
                     IQVRVAAHIYGSVRSTLYTTPLQEVPAPVYA"
     gene            1616..2164
                     /locus_tag="PLP58_19115"
     CDS             1616..2164
                     /locus_tag="PLP58_19115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007415822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF-type sigma factor"
                     /protein_id="HRJ10699.1"
                     /translation="MADLTLLLDAIQRGEPGATDELLTQVYAELRQLARAKMAREQPG
                     HTLQATALVHEAWLRLGDQRFENRAHFFAAAAEAMRRILIDRARRRQAARHGASAEHL
                     DVNEIEIAAPAGKDDELLALHDALTALEAHDARKAELVKLRYFAGLGFEEAAEILGIS
                     VPTAKRDWTYARAWLYQAIRSS"
     gene            2231..3856
                     /locus_tag="PLP58_19120"
     CDS             2231..3856
                     /locus_tag="PLP58_19120"
                     /inference="COORDINATES: protein motif:HMM:NF013053.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismatase family protein"
                     /protein_id="HRJ10700.1"
                     /translation="MRISALCLLAAASLAAETPLALTLQSRSSSGRPVLVKEQWPPSS
                     TAVIVCDMWDLHHCKNAVTRAVEMAPRMNEVLEKARAAGVLIIHAPSSCMKPYEGTPA
                     RERARTAPAAPRLPDKIGEWCKQIPAEEMAVYPLDQSDGGEDDDPEEHEKWAAELTAR
                     GLNPRAPWTKQINTLRIDQEKDAVSDSGVEIWNLLESRGIDHVILMGVHLNMCVSGRP
                     FGLRQMAKNGKHVVLMRDMTDTMYNPARWPYVSHTRGTEIFVQHVERLICPTITSDQL
                     IGGQPFAFSDATAGKKRLQIILLGDSTTEAGFPKKHAPDEPQFEDMLRIKLATEGDLP
                     PCDVYNEGVSGEFIRRLLDTRYDKAVKTKPQADYIFIRYGINDIARRENFAENFPKDF
                     REMLSRLRQDHPKAMLIPMTVIPYSATNTHDDINAAVRKIAAEEKLAVFDIAPRYLAE
                     LKKNPDGLNYRRFPLSKIPGNLRPLATPYITPEGAEPKVVVMDNRLDGLFGHLPGWAG
                     DRHPNLAGYNVIADETAKWLAPVIRKTQAPGGH"
     gene            3906..5021
                     /locus_tag="PLP58_19125"
     CDS             3906..5021
                     /locus_tag="PLP58_19125"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="sialidase family protein"
                     /protein_id="HRJ10701.1"
                     /translation="MKAVPLLSLLLCPALMALEPLVPKTRTTHHLAPAPEQAGGVYLA
                     FPALLALDDEVLVSCKHGRAHARDPGAALDLIRLDRASGKVLGTQSLAAHPDMTMQMG
                     EWVRFPNGDIANYSDVYDAEATLRSGMGVVRSTDGGRTFSPIERVGIVDGVEYGYPFD
                     FIVRGPATWMLVMNFEHLAGGRRVHPAKSRPGPVNVIRSDDNGRTWRFVRGITDELGG
                     APINESAFMPHGDGFLVTTRGYDDTQWLCRTDAEFRIIEKTDLNADNDFIGQHIGRPR
                     LFEKDGQCYLLGRNWVEKGLMQLALFKLDPATLCITRHVILDNAEKARLDDGYYAQGY
                     WTQRDGGESFHVITYKRSQGRHSPDILRLEFDWAEVR"
     gene            5092..6309
                     /locus_tag="PLP58_19130"
     CDS             5092..6309
                     /locus_tag="PLP58_19130"
                     /inference="COORDINATES: protein motif:HMM:NF012471.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M14-type cytosolic carboxypeptidase"
                     /protein_id="HRJ10702.1"
                     /translation="MIRLVSLFLTIIFVIAQARSDEAPSPPLDFIDTSFENASPLYYE
                     FAEDGTVQVFLLYDQERLSPNRAAGHFHFRIQASKGQKLTLELKNMANVWNSKAGSVA
                     KEIKQVVISQDGKNWTPVPTESPEEGRVRVTLEMPGDSLFVARAEPYRVSDLNRFLDE
                     IKASPLVRIETIGKTVQGRDLEIIRVGNDDAPNHVFIRARAHPWEPVGNWVVEGLVRR
                     LLKGDADAQKCLALYNVCILPMANKDGVERGGTRFNLGGWDLNRKWDKPADPKLAPEN
                     AALERWLESEIKAGRRPTFAMDLHNDGNGMLHISRPDIPDIDAYLRRMAHFEQCLRDH
                     TWFTVGSTKPSFRNPGTLGEGWFTRFGIDAVILEFNCNYIEGIKQPAMGKHWITFGEG
                     LTNAFEAYFEPRK"
     gene            6338..>7151
                     /locus_tag="PLP58_19135"
     CDS             6338..>7151
                     /locus_tag="PLP58_19135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009962965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HRJ10703.1"
                     /translation="MFSRLLLFSLLSFSLHAQPIGDGKADDTAAVQAMVNAGGAVRFE
                     RGTYRLTKPIVIDLDQTGFISLSGDGVARFVMEGARPAFHFKGTHGGTAAPTTLKPEI
                     FARQRTPMVDGIEIVGAHAGADGIEATGTMQFTITRVSVHEARHGIHLTVRNRNVIIS
                     DCHLYHNSGIGVFYDHVDLHQSNITGSHISYNAGGGVVTRGGGVRNLHIGTCDIESNM
                     APDAPPTANVLIDCTEGSTAEVSITGCTIQHNSVSPGSANVRFIGKGKPYGND"
BASE COUNT         1394 a         2411 c         2173 g         1173 t
ORIGIN      
        1 gccgccaccg ccatcctcgc catggccgag caccagcgaa accaccccgc cgaagcccgc
       61 gccgccctcg ccgccgccaa aaccgccctc gccgccaact ggcccgccgg caccgaagcg
      121 aactggtatg cctggctcat cgccgacctc ctcgcccgcg aagccgggac gttggtgggg
      181 aagtctcgct gatccttttc cgcgtgggat ttcgcatgag cattgagaga aacccgccca
      241 tgccaccgcc gccaccgccc cagcgtccat ccggcccaac gaaaccgccc gcgccgctca
      301 agccggagga agcgggggag cgcatcgggc cttgtaagct gctccagcag atcggcgagg
      361 gtggcaccgg aggccgggag gcgggtgaga tgaagcgtgc agtgcggggc aatgtggata
      421 gcttgcaccg catggacgaa ggctttctct accaaccggc tcccaatcca cgcggcggat
      481 tcacctgccg ggcggtgaag ggagagcaga tcaccgaggc tgagctggat gccgccgtgg
      541 ccgccgagac ggggctgccg ccggagcaat gcgcgcaggt cctgaaggcc tacctgcgcc
      601 agatgctggc caaaggggcc accagccgct ggtcgccggg gctgtatggc ctcatcggct
      661 tctaccccac ggcgggcggc aaccagccgg ggcacgatga cttccacacg gcggagcagg
      721 tgaaggcagg catgagcatc gccttcagcc tggatgccat ccgcgcctgg cggcaggggc
      781 tgcgcctggt gagccagggc ttcaccggac gggtgacacc gaagctggcg acggtgatct
      841 gctgcaccac ggacaagccg gacgtctatg tgtccggcca cctcatcggc gtgcgcggcg
      901 cccacctgaa gctggaccag cacgacccgg cccagggcat ctttttcatc ctggcggacg
      961 gcacggaagt gcgctgtgag acctatggcg gaaacacccg cctgcgtgtc tatggccggg
     1021 tgccggaggg tgtcaccggc cccatccagg tgcgggtggc cgcccacatc tatggcagcg
     1081 tgcgcagcac cctctacacc acccccctcc aggaggtgcc agccccggtg tatgcataaa
     1141 agcggccacc accccgcccc tgccagcgcc gcccccaggc aggcaaagcg gcggggggcg
     1201 ggtcaaagag gccgttgggg tgactccaac aggttcaagg atgccgggtg cgcgtgtcgc
     1261 gggtgccgta tgccatcccg tcggagtcac tccgacaagt tcatggatgc cacccagatc
     1321 agctcagggt gccgtatgcc atcccgtcgg agtcactccg acatgttcat agatgccacc
     1381 cacgtccgca cagggtgccg taggacaacc cgtcggagtc actccaacag cctccgtgcg
     1441 gcggggtcat cgccccggag aggggatggg cacttcctca ggggggctgc cgttgttctt
     1501 gcagcgcgga cgcgcccccc gcaccacccc ctctggcacc tgcccggcac gcccccggct
     1561 tgcgctgcga tttggactgg ccttcccccc tgccctccct cacactcccg ctgtcatggc
     1621 cgacctcaca ctactgctcg atgccattca gcgtggcgaa cccggcgcca ccgatgagct
     1681 gctcacccag gtctatgccg agctgcgcca gctcgcccgc gccaagatgg cgcgcgagca
     1741 gcccggtcac accttgcagg ccaccgcgct cgttcacgag gcctggctgc ggctgggaga
     1801 tcagcgcttt gaaaaccgcg cccatttctt cgccgccgcc gccgaggcca tgcgccgcat
     1861 cctcattgac cgcgcccgcc gccgccaggc cgccaggcat ggcgcgagcg cggagcatct
     1921 ggatgtgaat gaaatcgaaa tcgctgcccc cgccgggaaa gacgacgaac tgctcgccct
     1981 ccacgacgct ctcaccgccc tggaggcgca cgacgcccgc aaggccgagc tggtgaagct
     2041 gcgctacttc gccggcctgg gctttgaaga agccgccgag atcctcggca tctccgtgcc
     2101 cacggccaag cgcgactgga cttacgcgcg ggcatggctg tatcaggcca ttcgcagctc
     2161 atgacgggat ccagcggcgc agttccggcc gcggtgacaa atggcaggca cccggccgcg
     2221 tataggacgc atgcgcattt ccgccctttg ccttctcgcc gccgccagtc ttgccgcgga
     2281 gacgccgctc gcgctcacgc tgcaatcccg gtcctcctcc ggcaggcccg tgctggtgaa
     2341 ggagcaatgg ccgccgtcca gcaccgccgt catcgtgtgt gacatgtggg acctgcacca
     2401 ctgcaaaaac gccgtcaccc gcgcggtgga gatggctccg cgcatgaacg aagtgctcga
     2461 aaaggcccgc gccgccggag tgctcatcat tcacgcgcca agcagttgca tgaagcccta
     2521 cgagggcact ccagcccgcg aacgggccag gacagcaccg gccgcgccgc gcctgcccga
     2581 caagatcgga gagtggtgca aacagatccc cgccgaggag atggcggtat atccgctgga
     2641 tcaaagcgat ggcggcgagg atgacgatcc cgaagaacac gagaagtggg ccgccgaatt
     2701 gacggccaga ggtctgaatc ctcgcgcccc gtggacgaaa cagatcaaca cgctgcgcat
     2761 tgaccaggag aaggacgccg tcagcgacag cggcgtggag atttggaacc tcctcgaatc
     2821 aagaggcatt gatcatgtca tcctcatggg cgtgcatctg aatatgtgcg tgagcggccg
     2881 ccccttcggc ctgcgccaga tggcgaagaa tggcaaacat gtcgtgctga tgcgagacat
     2941 gaccgacacg atgtacaatc ccgcccgctg gccctatgtg agccacacgc gcggcacgga
     3001 gatcttcgtc cagcatgtcg agcgcctgat ctgccccacg atcacctccg accagctcat
     3061 cggcggccag ccctttgcct tcagcgatgc cacggcgggc aaaaagcgcc tccaaatcat
     3121 cctgctgggc gacagcacca ccgaggcggg ctttccaaag aaacacgctc ccgatgaacc
     3181 gcagttcgag gacatgctcc gcatcaaact ggccaccgag ggcgatttgc ctccttgtga
     3241 tgtctacaac gaaggcgtca gcggcgagtt catccgccgc ctgctcgaca cccgctacga
     3301 caaggcggtc aaaaccaagc cgcaggccga ctacatcttc atccgctacg gcatcaacga
     3361 catcgccagg cgggagaact tcgccgaaaa cttcccgaag gacttccgcg agatgctttc
     3421 ccgcctgcgc caggatcacc caaaggccat gctcatcccg atgaccgtca ttccgtactc
     3481 ggctaccaac acccacgatg acatcaacgc cgccgtgagg aagatcgccg ccgaggaaaa
     3541 gctcgcggtc ttcgacatcg cgccgcgcta cctcgccgag ctgaaaaaga atcccgatgg
     3601 cctgaactac cgccgctttc ccctttcgaa gattcccggg aacctccgcc cgctcgccac
     3661 gccttacatc acgcccgagg gcgccgaacc gaaggtcgtc gtgatggaca accgcctcga
     3721 cggcctcttc ggccacctcc caggctgggc cggagaccgg catccgaacc tcgcgggcta
     3781 caacgtcatc gccgatgaga cagcgaagtg gctcgcgccg gtcatccgaa agacgcaagc
     3841 tcccggtgga cactgaagcc ggagtgaggt cgaaaaagcg cgcggcattc gcgtagtatc
     3901 aggtcatgaa agcggtcccc ctgctgtccc tgctcctctg tcccgcgctg atggctctgg
     3961 agccgctggt gcccaaaacc aggacgacgc accacctcgc gcccgctcca gagcaggcgg
     4021 gcggggttta tctggccttc cccgcgctgc tcgcgctaga tgacgaggtg ctggtctcct
     4081 gcaagcacgg ccgcgcacat gccagggacc ccggcgcggc gctggacctc atccggctgg
     4141 atcgcgccag cggcaaggtg ctgggcacgc agtccctggc cgcgcatcct gacatgacga
     4201 tgcaaatggg cgagtgggtg cgctttccga acggtgacat cgccaattac agcgatgttt
     4261 atgacgcgga agcaaccctg cgctccggga tgggggtcgt gcgctccacc gacggcgggc
     4321 gcaccttcag ccccatcgag cgtgtgggga tcgtggacgg cgtggagtat ggctatccct
     4381 tcgacttcat cgtgcgcggc cccgccacct ggatgctggt gatgaacttc gagcatctcg
     4441 ccggaggccg ccgcgtgcat ccggcgaaaa gccgccccgg gccggtcaat gtgatccgca
     4501 gcgatgacaa cggccgcacc tggcgcttcg tgcgcggcat cacggacgaa ctgggcggcg
     4561 cgcccatcaa tgagagcgcc ttcatgccgc acggcgacgg gtttctggtc accacccgcg
     4621 gctacgatga cacgcagtgg ctgtgccgga cggacgccga attccgcatc atcgaaaaga
     4681 ccgacctgaa cgctgacaac gacttcatcg gacaacacat cggccgcccg cgtctgtttg
     4741 aaaaagacgg gcagtgctac ctgctcgggc gcaactgggt ggagaaggga ctgatgcagc
     4801 tcgccctttt caagctcgat cccgcgacac tgtgcatcac gcgccatgtc atcctcgaca
     4861 acgcggagaa agcccgcctg gatgacggct actatgcgca ggggtattgg acgcagcgcg
     4921 atggcggaga gtcgttccac gtcatcacct acaagcgctc tcaggggcgg cacagtccgg
     4981 acatcctcag gctggagttt gactgggcgg aggtgcgctg acaccccatt tctcatgccc
     5041 gatacttgca tccagccggg gcgttgaact ttcaaggcaa tttccaaaac catgatccga
     5101 ctcgtttcac tttttctgac aatcattttc gtcatcgccc aagcccgctc tgacgaggcg
     5161 ccatcaccac cgctcgattt catcgacaca agcttcgaga atgcctcgcc gctgtattat
     5221 gagttcgctg aggacggcac ggtgcaggtg tttttgctct acgatcaaga gcggctctcg
     5281 ccgaatcgtg cggcgggaca ttttcatttc cgcattcagg cctcgaaggg tcaaaagctc
     5341 acgctggagc tgaaaaacat ggccaatgtc tggaacagca aagccggctc ggtggcgaaa
     5401 gagatcaagc aggtcgtcat ctcgcaggat ggtaaaaatt ggacgcccgt gccgacggaa
     5461 tcgccggaag aaggccgcgt gcgcgtcacg ctcgaaatgc cgggtgattc gctcttcgtc
     5521 gcgagggccg agccgtatcg cgtgtcggac ttgaaccgct tccttgatga gatcaaagcg
     5581 agtccattgg tgagaatcga gacgattggc aaaacggtgc agggtcgcga tctggagatc
     5641 atccgcgtgg gcaacgacga cgcgccaaat catgtcttca tccgcgcacg cgcgcatccg
     5701 tgggagccgg tgggcaactg ggtcgtcgaa ggactcgtgc gccgtttgct gaaaggcgat
     5761 gcggatgcgc aaaagtgcct cgcgctctac aacgtgtgca ttctaccgat ggcgaacaag
     5821 gatggcgtcg agcgcggggg cacgcgattc aacctcggcg gctgggattt gaaccggaag
     5881 tgggacaagc ccgccgatcc taagctcgcg ccggaaaacg cggcgctgga gcgctggctc
     5941 gaaagcgaga tcaaagccgg acgccggccc accttcgcga tggacctgca caacgacggc
     6001 aacggcatgc tgcacatcag ccgcccggac attccggaca tcgacgccta tctcaggcgc
     6061 atggcacatt tcgagcaatg cctgcgcgac cacacctggt tcaccgtcgg gtccacgaag
     6121 ccctcgtttc gcaatcccgg cacgctcggc gagggctggt tcacgcgttt cggcattgat
     6181 gccgtcatcc ttgagttcaa ctgcaactac atcgaaggca tcaagcagcc cgccatgggc
     6241 aagcactgga tcacctttgg cgaaggcctc acaaacgcct ttgaggcgta ttttgagccg
     6301 cgcaaatgac cgtctcatgt gcgtatgaca tcgcctcatg ttctcgcgcc tgcttctctt
     6361 ctcgttgttg tccttctctc ttcacgccca gcccatcggt gatggcaagg cggatgacac
     6421 cgctgcggtg caagcgatgg tcaatgccgg tggcgcggtg cggtttgaac gcgggacgta
     6481 tcgcctgacg aagcccatcg tcattgacct tgatcaaacc ggcttcatct cgctcagcgg
     6541 cgacggcgtg gctcggttcg tgatggaagg cgcgagaccg gcgtttcatt tcaaaggcac
     6601 gcacggcggc acggcggctc ccacaacatt gaagccggag attttcgcgc ggcagcgcac
     6661 gccaatggtg gacggcatcg aaatcgtcgg cgcgcatgcc ggggcggatg gcatcgaggc
     6721 cacaggcacc atgcagttca ccatcacacg cgtgtcggtg catgaggcac gacacggcat
     6781 ccatctcacc gtgcgaaacc gcaatgtcat catcagcgac tgtcatttgt atcacaacag
     6841 cggcatcggc gtgttttacg accatgtcga tctccaccag tcgaacatca ccggcagcca
     6901 catcagctac aacgccggcg gcggcgtggt gacacgcggg ggcggcgtgc gaaacctcca
     6961 catcggcacc tgcgacatcg agagcaacat ggctcccgac gcaccgccga ccgctaacgt
     7021 gctcatcgac tgcaccgaag gctccacggc cgaggtcagc atcaccggct gcaccattca
     7081 gcacaacagc gtctcgcccg gctcggcgaa tgtgcgcttc atcggcaaag gcaagcccta
     7141 cggcaacgac g
//
LOCUS       DAOMYJ010000251         7028 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Prosthecobacter sp. isolate AMR_MDS_5629
            NODE_3614_length_7028_cov_18.774272, whole genome shotgun sequence.
ACCESSION   DAOMYJ010000251 DAOMYJ010000000
VERSION     DAOMYJ010000251.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867850
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prosthecobacter sp. (wastewater metagenome)
  ORGANISM  Prosthecobacter sp.
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Verrucomicrobiaceae; Prosthecobacter.
REFERENCE   1  (bases 1 to 7028)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7028)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.30
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:34:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,742
            CDSs (total)                      :: 4,684
            Genes (coding)                    :: 4,676
            CDSs (with protein)               :: 4,676
            Genes (RNA)                       :: 58
            tRNAs                             :: 55
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7028
                     /organism="Prosthecobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3614_length_7028_cov_18.774272"
                     /isolate="AMR_MDS_5629"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1965333"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..416)
                     /locus_tag="PLP58_19140"
     CDS             complement(<1..416)
                     /locus_tag="PLP58_19140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ10704.1"
                     /translation="MPEPSLFRHYQIVQDTDGRNVELVRDARQVAVLAFDTKRLEFVH
                     CHVLLEALADRPAFDKACRRLQTEGHPLLARMLDYGEDEGNPYFITSHVDGETLGQYL
                     ARQDELPAWLAAAIAARALEADEEDDQQVHPDKYAD"
     gene            599..958
                     /locus_tag="PLP58_19145"
     CDS             599..958
                     /locus_tag="PLP58_19145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ10705.1"
                     /translation="MTQPQREALFDILALAIYADAHVSLDEDELVRQAFVKRGWKSTR
                     PKSLFIDESFARAREAAESDDAMMDYLEERAASFTTKAAQKEVCALVKDILEGDGMTA
                     EENEFFHLLVQSLPRLK"
     gene            complement(1040..1309)
                     /locus_tag="PLP58_19150"
     CDS             complement(1040..1309)
                     /locus_tag="PLP58_19150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018970121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ10706.1"
                     /translation="MTDELVNVLSGGQTLEFNALFKLVYDNLKLKNAVSGGEEMLRLR
                     SYEKLQGLVSRGLCAKVGKTYRGLEGLRAAHQAALAARTAAIVAR"
     gene            complement(1527..2834)
                     /locus_tag="PLP58_19155"
     CDS             complement(1527..2834)
                     /locus_tag="PLP58_19155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009960005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HRJ10707.1"
                     /translation="MPALPKPLLFCVLPACLASAQPATTPLFWPDRTGPTNDGVVPAA
                     EAARLPLEWDGESGRNIAWRTEIDDAGHSTPVIGGDMIWFTSATEDGKKQFVYGIDRH
                     SGRVVHHKLLFENADPEPLGNPLNNYAAPSPVLEADALYVHFGTYGTARLDPATAEVV
                     WQRRDINVNHYRGPGSSPVIHEGLLILTLDGINEQFVTALDKKTGATVWKTNRTTDYG
                     DLDAEGKPTRDGDMRKAYHTPTVFDLAGVPTLVSVGSRAAFGYDPATGRELWTIRHGG
                     FNAAIRPLLIKNVLILNTGSERAHTLGVRVDAGMKGDITESHVLWDREKRNASEAGPV
                     LVGGLLFQITRGGILSCMNPLKGEDIWEDRLDGQHLPGPIAAGDRLYFCNDRGQSHVV
                     RAGDKFEILAKNQLPEAISAGMAVADGALYIRSKKALYKIAVK"
     gene            complement(2885..3370)
                     /locus_tag="PLP58_19160"
     CDS             complement(2885..3370)
                     /locus_tag="PLP58_19160"
                     /EC_number="1.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018971005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA];
                     GO_function: GO:0005542 - folic acid binding [Evidence
                     IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HRJ10708.1"
                     /translation="MMPRLAAIVAMDARRVIGRGGALPWHHPEDLRFFKRTTLGHPVL
                     MGRTTFESIGRPLPGRRNVVLSRAMPEREGVTVIRDLAELERACGGEEKVFVIGGAQV
                     YAELLPRCGEVYLTLVAGEHEGDAFLPPFEHLFDLKEVLGRTDELEFRRYERKRTEAA
                     G"
     gene            complement(3367..4392)
                     /locus_tag="PLP58_19165"
     CDS             complement(3367..4392)
                     /locus_tag="PLP58_19165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ10709.1"
                     /translation="MKTRALFLSLSLLTAMLPAQEAAEIASVQKIWDKGEHNAFTDLA
                     RFKNLFFCCFREAKAHVGGDGVIRVLISSTGENWVDYASIAEAGVDLRDPKFEVTPDG
                     KQLYLLCGGSVYEGTQLKGRRPRYVMSTDGKVWTPPQKLLAEGDWLWRVTVNPADGKF
                     YGAAYNCHPTTGGPKFEEEWSLKSYASADGRVWQLSSIMQVPGQPNETTIRFLKDGSA
                     VALVRREAGDRKGMIGVAAAPFRQWSWTPLPVPLGGPNFIELPDGSLVAGSRGFGRTP
                     GPHMVLYKMTKTGVEPLLELPSAGDCSYPGLVWHEGHLHVSYYSSHEGKSAIYHARVR
                     LPWVREP"
     gene            complement(4596..6140)
                     /locus_tag="PLP58_19170"
     CDS             complement(4596..6140)
                     /locus_tag="PLP58_19170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ10710.1"
                     /translation="MKQATLLAAALIGLGMHNRAHAATDNFENATVITGSSLNFTSVS
                     ILNYTSQAGEPPMTSWGEYGAKKTAWWKWTAPEDGFCTVDTLSPPNDMPVRDTLVAVY
                     TGASVNALTLIADGDDHWTRANGAAANGASCTFYATKGVTYHIAVDGYHASSVTATAH
                     NVTLRLGLLPKRGMRKYGVYRIVLADSQLHGTLSFTKTSAFSFSAKFTVGKKAYPLKG
                     VLTPEGFFMTAIERKTPAGAAPLPPIGLIIDAKAEGDLYVGEGRLYGARSNLMEAHTF
                     PAGVTTTVAGKFTGVLNTNGSEAPGVVCATVKGNGMVTLAGLAADGSKLTFSGPLVHD
                     HDASATGTLPVCVSLYGGGGFVRAHFHFTDNAMGDRLEASQTGHFRPANPASVFYPAG
                     IDSSLVVLGGSYAPEKNKRALDFLEGSMGAGKLSIPASMDEIASMISENLAFDTKNKF
                     IFPPKNARKPALKLNASGLVTGSFLDDNGKKRVVNGVLYRFNGQTLLRGQVAGATKNV
                     LFQVIP"
     gene            complement(6377..6904)
                     /locus_tag="PLP58_19175"
     CDS             complement(6377..6904)
                     /locus_tag="PLP58_19175"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: protein motif:HMM:NF020123.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HRJ10711.1"
                     /translation="MKLRPLVTTALVCLATAAAAPAGEGEKVSLPAPDVSGLNQDGVN
                     VKFTEVYAKGPTVVFFYPKANTPGCTKQACSLRDAFADLTKQGVQVLGVSFDKPEAQK
                     KFRDDFTLPYDLIADPEGRIVDGFGVKRMERGEVTLAARQAFLVKEGKIVWRDAQAST
                     DKQAEDILRVLKEVK"
BASE COUNT         1192 a         2411 c         2160 g         1265 t
ORIGIN      
        1 tcatcggcgt atttgtcggg atgcacctgc tgatcatctt cctcatcggc ctccagcgcg
       61 cgcgcggcga tggccgccgc cagccatgcg ggcagctcgt cctgccttgc caggtattgg
      121 cccagcgtct cgccgtccac atggctcgtg atgaagtatg ggttcccctc gtcctcgccg
      181 tagtcgagca tgcgcgccag cagggggtgc ccctccgtct gcaggcggcg gcaggctttg
      241 tcgaaggccg gccggtccgc cagcgcctcc aaaagcacat ggcaatggac gaactccagc
      301 cgcttcgtgt caaaggccag caccgccacc tggcgcgcgt cgcgcacaag ctccacattc
      361 cgcccgtccg tgtcctggac gatctggtag tgcctgaaaa gactgggttc cggcattgtg
      421 gcgtggggtg tgggtgagtg agggccgcag tgtcctaccg cctcaccgct tttcaagagc
      481 ggagtcggag acggcggcat cggtggacag ggctggcgcg cgggttgctg ggaaattggc
      541 ggcggaagca aaagctgtct tgtccggctc tgaggaatcc gctaaaaaac catcaaatat
      601 gacccagcca caacgcgaag cacttttcga catcctcgcc ctcgccatct acgccgacgc
      661 tcatgtctcc ctggacgagg acgagctggt ccggcaggct ttcgtcaaac ggggctggaa
      721 atccacccgg cccaaaagcc tgttcattga cgaatccttc gcccgcgccc gcgaggcggc
      781 ggaaagtgac gatgccatga tggactacct cgaggagcgc gccgcctcct tcaccacgaa
      841 agccgcgcaa aaagaggtct gcgcgctggt gaaggacatc ctggaaggcg acggcatgac
      901 tgcggaggaa aacgagtttt tccaccttct cgtccagtcc ctgcccaggc tgaaatgaat
      961 ccagcccggc aaaaagggcc gtcctgagca aaggacggcc ctggttttga ggaaggggtg
     1021 gaaacggctg aaggatggct cagcgcgcga cgatggcggc ggtgcgggcg gcgagcgcgg
     1081 cctggtgggc ggcgcgcagg ccttcgaggc cgcggtaggt tttgccgact ttggcgcaca
     1141 agccccggct gaccaggccc tgcagcttct cgtaggagcg gaggcggagc atttcctcgc
     1201 cgccgctgac cgcgttcttc agcttgaggt tgtcatagac cagcttgaag agcgcattga
     1261 actcaagcgt ctggcctccg gagaggacat tgacaagctc gtcggtcaca tgatcaggga
     1321 cgcggcggga gaaacgtgtt ttacgggtcg gtgtcgttgc catagaggct gccagtctaa
     1381 cacagacaat gacgaatgcg acggttttgt gcatttgcgg ggatttaatt ttcccatgag
     1441 gccggatttg aaagaatctg gccgatcctg cgtttttttt tcgcctccga cgcggattgt
     1501 atgcacaaag catggtcaaa ttgcagtcat ttgaccgcga ttttatacaa cgcctttttc
     1561 gagcggatgt agagcgcccc gtccgccacg gccattcccg ccgaaatcgc ctccggcaac
     1621 tggttcttcg ccaagatttc gaatttgtca cccgccctca ccacatggct ctggccgcgg
     1681 tcgttgcaga agtaaaggcg gtcccctgcc gcgatcggcc ccggcaggtg ctgcccgtcg
     1741 aggcggtcct cccagatgtc ctcgcccttg agcggattca tgcaggagag gatgccgccg
     1801 cgtgtgatct ggaaaagcag cccgcccacc agcacggggc cggcctcgct ggcgttgcgc
     1861 ttttcccggt cccaaaggac atggctttcg gtgatgtccc ccttcatgcc cgcatccacc
     1921 cgcacaccga gtgtatgggc gcgctcggag ccggtgttga gaatgagcac gttcttgatg
     1981 agcaggggcc ggatggccgc gttgaagccg ccgtggcgga tggtccacag ctcccggcct
     2041 gtggcgggat cgtagccgaa ggccgcccgc gagccgacgc tgaccagcgt gggcactccc
     2101 gccaggtcaa agaccgtcgg cgtgtggtag gccttgcgca tgtcgccatc gcgcgtcggt
     2161 ttgccctcgg cgtccaggtc gccgtagtcg gtggtgcggt tcgttttcca caccgtggcg
     2221 ccggttttct tgtccagcgc ggtgacgaac tgctcattga tgccatccag cgtgagaatc
     2281 aggagaccct cgtggatgac gggcgagctg cccgggccgc ggtagtggtt cacattgatg
     2341 tcccgccgct gccagaccac ttcggcggtg gccgggtcca ggcgcgcggt tccgtaagtg
     2401 ccgaaatgga catacagcgc gtccgcctcc agcacggggg agggcgcggc gtagttgttg
     2461 agcgggttgc ccagcggctc ggggtccgcg ttttcaaaga gcagcttgtg atggaccacg
     2521 cgcccgctgt ggcggtcaat gccatagacg aactgcttct tcccgtcctc cgtggcgctg
     2581 gtgaaccaga tcatgtctcc gccgatcacc ggcgtgctgt ggcccgcgtc atcgatctcc
     2641 gtccgccaag cgatgtttct gccgctctcc ccatcccact ccagcggcag ccgcgccgcc
     2701 tccgccgccg gcacgacacc gtcattcgtc ggccctgtgc ggtccggcca gaaaagcggc
     2761 gtcgtcgccg gctgggcgga cgcgaggcag gcgggcagga cgcaaaagag aagtggcttg
     2821 ggcagggcgg gcatggctgg aaaggacggc ggggcgcgca gggttctatc gctccccggc
     2881 caagtcaacc cgccgcctcc gtccgcttgc gctcatagcg gcggaattcc agttcgtccg
     2941 tcctgcccag cacctccttg aggtcaaaca gatgttcgaa gggcggcagg aaggcgtccc
     3001 cctcatgctc gccggccacc agtgtcaggt acacctcccc gcagcgcggc agcagctccg
     3061 catagacctg cgcgccgccg atgacgaaca ccttctcctc cccgccgcac gcccgctcca
     3121 gctccgccag gtcccggatc accgtcaccc cctcccgctc cggcatggcc cggcttagca
     3181 ccacattgcg ccggcccggc agcggcctgc cgatggactc aaacgtcgtc ctgcccatca
     3241 gcaccggatg ccccagcgtc gtgcgtttga aaaagcgcag gtcctccggg tgatgccaag
     3301 gcagcgcccc gccgcgtccg atgacacggc gcgcgtccat ggcgacgatg gcggccaggc
     3361 gcggcatcat ggctccctca cccagggcag gcgcaccctg gcgtgataaa tggccgactt
     3421 cccctcgtgg ctggaataat agctcacatg cagatgcccc tcgtgccaga ccaggcccgg
     3481 atagctgcag tcccccgcgc tcggcagctc cagcagcggc tccacgcccg tcttcgtcat
     3541 cttatagagc accatgtgcg gccccggcgt cctgccaaag ccgcgcgacc ccgccaccag
     3601 cgacccgtca ggcagctcga tgaagttcgg cccgcccaaa ggcaccggca gcggcgtcca
     3661 tgaccattgg cggaaaggcg ccgccgccac gccgatcatg ccctttctgt cacccgcctc
     3721 ccgccgcacc agcgccaccg ccgagccgtc cttcagaaag cggatcgtcg tctcattcgg
     3781 ctggcccggc acctgcatga tggaggagag ctgccacacc cggccatccg cgctggcata
     3841 cgacttcaaa gaccactcct cctcgaactt cggcccgccc gtcgtcgggt ggcagttgta
     3901 ggccgccccg taaaacttcc cgtccgccgg gttcaccgtc acccgccaca gccagtcccc
     3961 ctccgccagc agcttctgcg gcggcgtcca caccttcccg tccgtggaca tcacatagcg
     4021 cgggcggcgg cctttgagct gcgtgccctc atacaccgag ccgccgcaca gcaggtagag
     4081 ctgcttgccg tccggcgtca cctcgaactt cggatcccgc aggtccacgc ccgcctccgc
     4141 gatgctcgcg tagtccaccc agttctcacc cgtcgaggag atcagcaccc ggatcacccc
     4201 gtccccgccc acatgcgcct tcgcctcgcg gaagcagcag aaaaacaggt ttttaaaacg
     4261 cgccaggtcc gtgaaagcat tgtgctcacc cttgtcccag atcttttgca ccgaggcgat
     4321 ctccgccgcc tcctgcgcgg gcagcatggc ggtcagcagg gacaggctca aaaacaaggc
     4381 acgggttttc atcagggcag tcatacgcgg gcgcgggcgg gctttcgccg ccgatcatgc
     4441 cgaacggcgg cctcctgtcg aatgggaact cgcgcgcgtc cgcgccatcc ccataaaaac
     4501 gaccgccccc ggcggacacc ccgcgcggtg atccgcgccc gggcatccac aggaggcagt
     4561 tattccggca tgcggctcac acgccgcccc ccggctcagg ggatgacctg aaacaggaca
     4621 ttcttcgtgg cgcccgccac ctggccgcgc agcagggtct ggccgttgaa gcggtagagc
     4681 acgccgttga ccacgcgctt tttgccgttg tcatccagga agctgccggt gaccaggccg
     4741 gaggcgttca gcttcagggc gggtttccgc gcgttcttgg gcgggaagat gaacttgttc
     4801 ttcgtgtcaa aggcgaggtt ctcgctgatc atgctcgcga tctcgtccat cgaggccggg
     4861 atgctcagct tgccagcgcc catggagcct tccaggaagt ccagcgcgcg cttgtttttc
     4921 tccggcgcgt agctgccgcc cagcacgaca agactgctgt cgatgccggc gggataaaac
     4981 acagaggcag gattcgccgg acgaaaatgc cccgtttgcg acgcctccaa ccggtctccc
     5041 atggcgttgt cggtgaaatg aaaatgagcg cgcacaaagc cgccgccgcc gtacaggctc
     5101 acgcacacgg gcagcgtgcc ggtggcactg gcgtcatgat catggaccag ggggccggag
     5161 aaggtcagct tggagccgtc ggcggcgagg cccgccagcg tgaccatccc gttgcccttg
     5221 acggtggcgc agacgactcc cggagcctcc gagccgttcg tgttcagcac gcctgtgaat
     5281 ttccccgcca ccgtggtggt gacacccgcc gggaaagtgt gcgcctccat caagttgctc
     5341 cgggcgccgt aaagcctgcc ctcccccaca tacaaatcac cctcggcctt ggcgtctatg
     5401 atcaggccga tcggcggcag cggcgcggct ccggcaggag tcttgcgctc gatcgccgtc
     5461 atgaagaatc cttccggcgt cagcacaccc ttcagcggat aggccttttt gcccaccgtg
     5521 aacttggcgc tgaagctgaa tgccgaggtc ttggtgaagg acagcgtgcc gtgaagctgg
     5581 gagtcagcca agacgatgcg atacacgccg tatttgcgca tgccgcgctt cggcagcagg
     5641 ccgagcctca gggtcacgtt gtgcgccgtc gctgtcaccg agctggcatg gtagccgtcc
     5701 accgcgatgt ggtaggtcac gcccttcgtc gcgtaaaagg tgcagctcgc tccattcgca
     5761 gctgccccgt tggcgcgcgt ccagtggtcg tcaccgtcgg cgatgagggt cagcgcgttc
     5821 acggaggcgc ctgtgtaaac cgccaccaaa gtgtcccgca ccggcatgtc gttcgggggc
     5881 gacagagtgt ccaccgtgca gaagccgtcc tccggcgccg tccacttcca ccaggcggtt
     5941 ttcttcgcgc catactcacc ccagctggtc atcggcggct cgccggcctg gctcgtgtaa
     6001 ttgaggatgg aaacactggt gaaattcagc gacgagccgg tgatgaccgt cgcgttctca
     6061 aagttgtcgg tcgcggcgtg cgcgcggttg tgcatgccca gcccgatcag ggcggcggcg
     6121 aggagtgtgg cttgtttcat ggcggatggg atgtggtgtg ttgtcttcat gacaccccca
     6181 ccctgacccc gccccgcgaa acagggtatg ggactttggt cggatgcgcg gcacatttta
     6241 tatccgacta aagtcggatg cgcgccgccc gcccgcggcg cccttttccg gccgcttcgg
     6301 gagcaaaaaa aagccgggcg tcaccgcccg gccagtcaca tgccccgccc tccggcggag
     6361 gcgcgtcaca cacccctcac ttcacctcct tgagcacgcg caggatgtcc tccgcctgct
     6421 tgtccgtcga ggcctgcgcg tcgcgccaca cgatcttccc ctctttcacc agaaacgcct
     6481 gccgcgccgc cagcgtcacc tccccccgct ccatgcgctt caccccgaag ccgtccacga
     6541 tcctcccctc cgggtccgcg atcaggtcat acggcagcgt gaaatcatcc cggaacttct
     6601 tctgcgcctc aggcttgtcg aacgacaccc ccagcacctg caccccctgc ttcgtcaggt
     6661 ccgcgaacgc gtcccgcagc gagcacgcct gcttcgtgca gcccggcgtg ttcgccttcg
     6721 ggtagaaaaa caccaccgtc ggccccttcg catacacctc cgtgaacttc acattcacac
     6781 cgtcctggtt cagcccgctc acatccggcg ccggcaggct gactttctca ccctcccccg
     6841 ccggagcggc ggcggcggtg gcgaggcaaa caagcgcggt ggtcacaaga gggcggagtt
     6901 tcatggcgga gtggatgagg gtggaatgtt accggcaggc gcgcgggaga gtttggacac
     6961 ccgcgcggcg gctccacctt caaacgcccg gacacccgca ttattccgcc cccgcgcatt
     7021 tttttccc
//
LOCUS       DAOMYJ010000252         7013 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Prosthecobacter sp. isolate AMR_MDS_5629
            NODE_3624_length_7013_cov_21.469531, whole genome shotgun sequence.
ACCESSION   DAOMYJ010000252 DAOMYJ010000000
VERSION     DAOMYJ010000252.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867850
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prosthecobacter sp. (wastewater metagenome)
  ORGANISM  Prosthecobacter sp.
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Verrucomicrobiaceae; Prosthecobacter.
REFERENCE   1  (bases 1 to 7013)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7013)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.30
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:34:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,742
            CDSs (total)                      :: 4,684
            Genes (coding)                    :: 4,676
            CDSs (with protein)               :: 4,676
            Genes (RNA)                       :: 58
            tRNAs                             :: 55
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7013
                     /organism="Prosthecobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3624_length_7013_cov_21.469531"
                     /isolate="AMR_MDS_5629"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1965333"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..956
                     /locus_tag="PLP58_19180"
     CDS             <1..956
                     /locus_tag="PLP58_19180"
                     /inference="COORDINATES: protein motif:HMM:NF021578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Na-translocating system protein MpsB"
                     /protein_id="HRJ10712.1"
                     /translation="GRGGPNARAIAEMLNDPRVRAALKERGISVPEDTVFVGGMHNTA
                     NDNLTFYDVDRVPVTHQFEFEVACKAAQEAAVRNAHERARRFGSASLGLSPAAAKRHM
                     EGRAEDLSQVRPEWGHATNSICIVGRRAKTRGLFLDRRAFLVSYDPMQDDGEKAILTR
                     ILGAVVPVCSGINLEYYFSYVDSPGWGSGSKLPHNITGLLGVMDGAMSDLRTGLPWQM
                     VEIHEPVRLLFVIETTPEAMRRILEKNPVIQTMVGNRWVQLAVMHPETGEIRVHHDGE
                     FEPHVCQSGPLPVAASSREWYAGWRDHLEFAEILPPAAGHN"
     gene            966..2342
                     /locus_tag="PLP58_19185"
     CDS             966..2342
                     /locus_tag="PLP58_19185"
                     /inference="COORDINATES: protein motif:HMM:NF012581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proton-conducting transporter membrane subunit"
                     /protein_id="HRJ10713.1"
                     /translation="MPATDSLLTTLGLLVVAAPALLLAVFFTAFLLNHRMQEETIGRL
                     IQGTISVSLLAALGAAGVMLARGVPQFVIELGDWVITPDYHLKFQFILDLLSLSYSIL
                     TLVLCATIGAFSTRYLHRERGYNRFFVIFAVFLMGMVTAALSDTVETLFAGWEMVGLS
                     SVLLIAFFQERPAPPRNGLRVWIVYRVCDAALMLAAIMMHEINGAGDFDHITGRHAWP
                     EHDPLLIGPHFALLGVLFIIAAAGKSALVPFSGWLPRAMEGPTPSSAVFYGALSVHLG
                     VFLLLRISPVLDSAPWLSVLVVLLGLATAAFAYLAGRVQTDIKSALAFASLTQVGIII
                     AEIGLGFRYLALVHLLGHACLRTLQFIRAPSLLHDHHQLEDAIGTRPREERAQWEQRL
                     PAAFQRALYRFSLERGYLDTILDRFITRPFVALFSWFDGLETRWAAMLEGGRENKPEA
                     ARTVNDRP"
     gene            2339..3700
                     /locus_tag="PLP58_19190"
     CDS             2339..3700
                     /locus_tag="PLP58_19190"
                     /inference="COORDINATES: protein motif:HMM:NF012581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proton-conducting transporter membrane subunit"
                     /protein_id="HRJ10714.1"
                     /translation="MTAGSFPWLELTLLAPLLGAGVTAFIKTPAAAARWAIGFILAVS
                     AGALGTAVEFLTADAAAVSSHGTFKADEIAAPMLPVLAVLHLLTLLGTSKSRMTPSFC
                     ARLLLSAFATMAAVTSQSGSVLIALMIAAALLPVWDLKSRGRRARGFLFYVVPFIGLL
                     LIGWTFSDGARPVWAVGLLLLALKLCGGIVPLHGWLPTLFQRASFGAAMLFVLPLIEV
                     VASLRLLLPHAPEWMLDAVSIACLVTAVYAAGMAVVQNDARRFYAFLALSQTSMVMFA
                     VMLHTPTSITAALCLWISIILALAGLGYSIRALESRFGSLSLREHHGYYEQAPGLAVI
                     FLVAGLASVGFPGTIGFVPMELLISGSAQHGLGFSVTLAVAAMLNGIAILRAYFSIFT
                     GRRPTTSVSLRETRTERGGIILIALAVFFGGWFSPRVVESRHRVADRLLEARGAVPGK
                     AGH"
     gene            complement(3718..4155)
                     /locus_tag="PLP58_19195"
     CDS             complement(3718..4155)
                     /locus_tag="PLP58_19195"
                     /inference="COORDINATES: protein motif:HMM:NF021983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2452 domain-containing protein"
                     /protein_id="HRJ10715.1"
                     /translation="MDHDPINEDLAQTAPRGNFLPYPASRLAPQIIPQDLTNFKSRGV
                     SRVERELQQELVELRERYLEVIDAFNWNKLIYEARFGFEPVVGEVYHLYEVEGRHHLS
                     MIEPESWHQKWIGTFRLNADGRWQVDKISQDFDLRAWVSTSVG"
     gene            4281..5039
                     /locus_tag="PLP58_19200"
     CDS             4281..5039
                     /locus_tag="PLP58_19200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ10716.1"
                     /translation="METPPAPAPASRRSSGCLKLLFATLLLSLVAGGLLLWKLADMAR
                     EGVSWLALLPEKMTSTIVTESFRQSVTEIASTNGAVLEVATLQTDETVTKHDSRGLFN
                     DSIPLGTTVSEIRTPVFYRYHILLSDEWRIEINDGHAIVHSPAIRPSQPPSIRTEGME
                     KKSTAGWLRFNAAENLASLESGLTTTLEKRAGSTKNLNLVREKARLSVAGFVREWLLK
                     DERGRAAQLKSLTIHFPDEAVPAATTLNFDDPAP"
     gene            5128..5940
                     /locus_tag="PLP58_19205"
     CDS             5128..5940
                     /locus_tag="PLP58_19205"
                     /inference="COORDINATES: protein motif:HMM:NF016330.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SWIM zinc finger family protein"
                     /protein_id="HRJ10717.1"
                     /translation="MWWSYSDAKERKNKLQREITRRQKRGETFTALQAPKGIKLSKTF
                     WGQAWCRNLESYQQYENRLPRGRSYLRQGNVYNLRIQQGLLSAQVAGSELYETRIHIQ
                     PLQRPQWKAIQKACAGQVNSMLDLLAGRLGDGVMAVLTDKDGGLFPKPKEIRFDCSCP
                     DYADMCKHVSAVLYGVGVLLDEKPELLFTLRGVDQAELLSEAGQSAVSGLDQAPSDLG
                     DADLSALFGIDLSGAESVPSVVERKPAGKRAGVKKQAVKPAKKAARKKSGSS"
     gene            6031..6267
                     /locus_tag="PLP58_19210"
     CDS             6031..6267
                     /locus_tag="PLP58_19210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009964376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ10718.1"
                     /translation="MSAPEESKDTRLKQAEKIASHPEQYKVCEGCGSIVVARAVTCPS
                     CHGYLFDATPERVVSQARELALRAPHSVLSADLS"
     gene            6318..6869
                     /locus_tag="PLP58_19215"
     CDS             6318..6869
                     /locus_tag="PLP58_19215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009958207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ10719.1"
                     /translation="MNPPADSPLPYSDVKPVGAADFYSAVNATFRFILRKTGMEGLRR
                     YWAELGRGYYAPVADRWRTGGLPAVAAYWRAFFDAEPGAVAEVELRRDEVVVEVGTCP
                     AIGYLRAHGREITPCFCQHCYFVSEAIGQGAGVEVRVSGGDGRCVQRFAKAGHFTAPQ
                     ALEEIRAATSLGTAETSSTNSNA"
BASE COUNT         1101 a         2435 c         2226 g         1251 t
ORIGIN      
        1 gcgggcgcgg cgggccgaac gcgcgcgcca tcgccgagat gctgaacgac ccgcgcgtgc
       61 gcgccgcttt gaaagagcgc ggcatcagcg tgccggagga caccgtcttc gtcggcggca
      121 tgcacaacac ggcgaatgac aacctcacct tttacgatgt ggaccgcgtg cccgtgactc
      181 atcaattcga attcgaggtg gcctgcaaag ccgcgcagga ggccgccgtg cgcaacgccc
      241 acgagcgcgc gcgccggttt ggctccgcct cactcggcct cagcccggcg gcggcaaagc
      301 gccacatgga gggccgcgcg gaggacctct cccaggtgcg gcccgaatgg gggcacgcca
      361 ccaactccat ctgcatcgtc gggcggcggg ccaaaacgcg cgggcttttc ctcgaccggc
      421 gcgccttcct cgtctcgtat gatcccatgc aggacgatgg ggagaaagcc atcctgaccc
      481 gcatcctcgg cgcggtggtg ccggtgtgct cgggcatcaa cctcgaatat tacttctcct
      541 atgtggactc gccgggctgg ggcagcggct ccaagctgcc gcacaacatc accggcctgc
      601 tcggcgtcat ggacggcgcc atgagcgacc tgcgcaccgg ccttccctgg cagatggtgg
      661 agattcacga gccggtgcgg ctgctctttg tcatcgagac cacaccggag gccatgcggc
      721 gcatcctgga gaaaaacccc gtcatccaga ccatggtagg caaccgctgg gtgcagctcg
      781 ccgtcatgca tcccgagacc ggggagatcc gcgtccatca tgacggcgaa ttcgagccgc
      841 atgtctgcca gtccggcccg ctgcccgtgg ccgcctcgtc ccgggaatgg tacgccggct
      901 ggcgcgacca cctggagttt gccgaaatcc tgccgcccgc cgccggccac aactgaaccc
      961 gcgcaatgcc cgccaccgac agcctgctca ccacgctcgg actcctggtc gtcgccgcgc
     1021 ccgcgctgct gctggccgtg tttttcaccg ccttcctgct caaccaccgc atgcaggagg
     1081 agaccatcgg gaggctgatc cagggaacga tctcggtcag cctgctggcc gcgctggggg
     1141 ccgccggtgt catgctggcg cgcggggtgc cgcagtttgt catcgagctg ggggactggg
     1201 tcatcacgcc ggattaccac ctgaaattcc agttcatcct ggacctgctc tcgctgtcct
     1261 actccatcct cacgctcgtg ctgtgcgcca ccatcggcgc attctccacg cgctacctgc
     1321 accgcgagcg cggctacaac cgcttcttcg tgatcttcgc cgtcttcctc atgggcatgg
     1381 tcaccgccgc gctttcggac acggtggaga cgctcttcgc ggggtgggaa atggtcggcc
     1441 tctcctccgt gctgctcatc gcctttttcc aggaacgccc cgcgccgccg cgcaacggcc
     1501 tgcgcgtttg gattgtctat cgcgtgtgcg acgccgcgct catgctcgcc gccatcatga
     1561 tgcacgagat caacggcgcg ggcgacttcg accacatcac cggcaggcat gcgtggccgg
     1621 agcacgaccc tctgctcatc gggcctcact tcgccctgct cggcgtgctc ttcatcatcg
     1681 ccgcagcggg caagtccgcg ctggtcccct tctccggctg gctgccgcgc gcgatggagg
     1741 ggcccacacc ctccagcgcg gtgttttacg gcgcgctctc cgtgcatctc ggcgtcttcc
     1801 tcctgctgcg catcagcccg gtgctcgaca gcgccccgtg gctgtccgtg ctcgtcgtgc
     1861 tgctcggcct ggccaccgcc gccttcgcct acctggccgg gcgcgtgcag accgacatca
     1921 aatccgcgct ggccttcgcc tcgctcacgc aggtgggcat catcatcgcg gaaatcggcc
     1981 tcggtttccg ctacctggcg ctcgtccacc tgctcggcca cgcctgcctg cgcacgctgc
     2041 aattcatccg cgctcccagc ctgctgcacg accaccatca actcgaggac gccatcggca
     2101 cccgcccgcg cgaggagcgc gcgcagtggg agcagcggct gcccgccgcc ttccagcgcg
     2161 cgctttaccg cttctcgctc gagcgcggct acctcgacac cattctcgac cgcttcatca
     2221 cgcggccctt cgtcgccctg ttctcctggt tcgacggcct ggaaacccgc tgggccgcca
     2281 tgctcgaggg cgggcgtgaa aacaaaccgg aggcggcgcg gaccgtgaac gaccgcccat
     2341 gaccgccggc tctttcccct ggctggaact cacgctgctg gcgccgctgc tcggcgccgg
     2401 cgtcaccgcc ttcatcaaaa cgcccgcggc ggcggcgcgc tgggccatcg gcttcatcct
     2461 cgcggtgtct gccggcgcgc tcggcacggc ggtggagttt ctcaccgcgg acgcggcggc
     2521 ggtttcaagt cacggcactt tcaaggccga tgaaatcgcc gcgcccatgc tgccggtgct
     2581 cgcggtgctg catttgctga ccctgctggg cacgtcgaag tcgcgcatga cgccctcgtt
     2641 ttgcgcgcgg ctgctgctct ccgcctttgc caccatggcc gccgtcacca gccagagcgg
     2701 ctcggtgctc atcgccctca tgatcgcggc cgccctgctt ccggtgtggg atttaaaaag
     2761 ccggggcagg cgcgcgcgcg gctttctttt ttatgtcgtg cccttcatcg gcctgctgct
     2821 catcggctgg accttttcag acggcgcgcg cccggtctgg gccgtcggcc tgctgcttct
     2881 ggcgctgaag ctgtgcggcg gcatcgtgcc cctgcacggc tggctgccca ccttgtttca
     2941 aagagcgtcc tttggcgccg ccatgctctt cgtgctgccg ctcatcgagg tggtggcctc
     3001 cctgcggctg ctgctcccgc acgcgccgga gtggatgctc gacgcggtca gcatcgcctg
     3061 cctcgtcacc gccgtgtacg ccgccggaat ggccgtggtg cagaatgacg cgcgccgttt
     3121 ttatgccttc ctggcgctga gccagacctc catggtcatg ttcgccgtca tgctgcacac
     3181 gcccaccagc atcaccgccg cgctctgtct gtggatctcc atcatcctcg ccctggccgg
     3241 cctcggctac tccatccgcg cgctggagtc gcgcttcggc tcgctgtccc tgcgcgagca
     3301 ccacggttac tacgagcagg cgccggggct cgccgtcatc ttcctcgtcg ccgggctggc
     3361 cagtgtcggc ttccccggca ccatcggttt tgtgcccatg gagttgctca tcagcggcag
     3421 cgcgcagcac gggctgggct tcagcgtcac gctcgccgtg gccgccatgc tcaacggcat
     3481 cgccatcctg cgcgcctact tttccatctt caccggcagg cgtcccacca cctccgtctc
     3541 gctccgggag acgcggaccg agcgcggcgg catcatcctc atcgccctgg ccgttttctt
     3601 cggcggctgg ttcagcccga gggtcgtcga gtcccggcac cgcgtcgcgg accggctgct
     3661 ggaggccagg ggcgcagtgc ccggcaaagc ggggcattga gccagccgcc gcatgcctca
     3721 cccgacgctg gtgctgaccc aggcgcgcag gtcgaagtct tgggaaatct tgtccacctg
     3781 ccagcgcccg tcggcgttga ggcggaaggt gccgatccac ttttgatgcc atgactccgg
     3841 ctcgatcatg ctgaggtggt gcctgccctc cacctcgtaa aggtgataca cctctccgac
     3901 gaccggctcg aatccgaagc gggcctcgta gatgagcttg ttccagttga aggcgtcaat
     3961 aacttctagg tagcgctcgc gcagttccac gagctcctgc tgcagttctc tctccactcg
     4021 ggagacaccc cggcttttga agttcgtgag gtcctgcggg atgatctgag gtgccaggcg
     4081 cgaggcaggg taggggagga aattgccgcg cggggcggtt tgggcgaggt cttcgttgat
     4141 cgggtcgtgg tccatgaggg ctggatgata cggcgcgaag tcagccaggg acggaatgga
     4201 atcaaccgga tgcgggccgg gtcccgccct ggccgcgcct tgcgccgcgt cattgtttgc
     4261 ggcagtttat cttgatggac gtggaaaccc cgccagctcc cgcccccgcc agccgccgct
     4321 cctccggctg cttgaagctc ctttttgcca ccctcctcct gagtcttgtc gcgggcggct
     4381 tgctgctgtg gaagctggct gacatggctc gggagggtgt ctcatggctg gccttgctgc
     4441 cggagaaaat gacctccacg atcgtcacgg agagcttccg ccagtcggtg accgagatcg
     4501 cctccaccaa tggcgcggtg ctggaggtgg ccacattgca gacggacgag acggtcacga
     4561 agcacgactc gcgcggactt tttaatgaca gcatcccgct gggcaccacc gtctcggaaa
     4621 tccgcacgcc ggtgttttac cgctaccaca tcctgctctc cgacgagtgg cgcatcgaga
     4681 tcaacgacgg ccacgccatc gtccactctc ccgccatccg gccctcccag cctccgtcca
     4741 tccgcaccga gggcatggag aaaaaatcca ccgccggctg gctgcgcttc aacgcggcgg
     4801 aaaacctcgc cagcctcgag tccggcctga ccacaactct cgaaaaacgc gccggctcca
     4861 ccaaaaacct caacctggtg cgcgagaaag cgcgcctgtc cgtggccggt ttcgtgcgtg
     4921 aatggctgct caaggacgag cgcggcaggg ctgcgcaact gaaaagcctc accattcatt
     4981 tccccgacga ggccgtgccc gccgcgacca ctctgaattt tgacgacccg gctccctgac
     5041 ctccgccgcc ggcaggcgcg cgatccagcg gggacggtgg ttttgacgga tttgacggcg
     5101 gtgaagcgcg cgtgtaaacc ggtcgtcatg tggtggtcct actccgatgc caaagaacgc
     5161 aaaaacaagc tccagcgcga gataacccgc cgccagaagc gaggcgagac attcaccgcg
     5221 ctgcaggcgc cgaagggcat caagctcagc aagaccttct gggggcaggc ctggtgccgg
     5281 aatctggagt cctaccagca atatgaaaac cgcctgccgc gcgggcgcag ctatctgcgc
     5341 cagggcaatg tttacaattt gcgcatccag cagggcctgc tgagcgcgca ggtggccggc
     5401 tcggagcttt acgaaacgcg catccacatc cagcccctgc agcggcccca gtggaaggcc
     5461 atccaaaagg cttgcgccgg gcaggtgaac tccatgctcg acctgctggc ggggcggctc
     5521 ggcgatggtg tcatggcggt gctcaccgac aaggacgggg ggctttttcc caagccgaag
     5581 gagatacgct ttgactgctc atgcccggat tacgcggaca tgtgcaagca tgtctcagcc
     5641 gtgctttacg gcgtgggcgt gctgctcgac gaaaagccgg agctgctctt caccctgcgc
     5701 ggcgtggatc aggcggagct gctttccgaa gccgggcagt cggcggtgtc cggcctggac
     5761 caggcgccct cagaccttgg agacgcggac ctttccgcct tgttcggcat tgacctctca
     5821 ggggcggaaa gcgtcccctc cgtcgtggaa aggaagcccg cggggaaacg cgccggcgtg
     5881 aaaaaacaag ccgtgaagcc ggccaaaaaa gcggcgcgga aaaagtccgg ctcatcgtga
     5941 gcgcaggcac gaacagcgtg gggcgcgcgc gatcccgcgc aaacagccgc gttgcattcc
     6001 ggctccgatg cgcgtatgag atcatgcaaa atgagcgccc ctgaagaatc caaggacaca
     6061 cgcctcaaac aggcggaaaa aatcgcgagc cacccggagc agtacaaagt gtgcgaaggc
     6121 tgcggctcga tcgtcgtcgc gcgcgcggtc acctgcccga gctgccacgg ctacctattc
     6181 gacgccacgc cggagcgcgt ggtgtcgcag gcgcgggagc tggcgctgcg cgccccgcat
     6241 tcggtgctga gcgcggacct cagttgaccg agcgccatca gggaaaagac caaggagacc
     6301 ccggcggaca ccacgagatg aatcctcccg ccgactcgcc cctgccttac tcggatgtga
     6361 aaccggtggg cgccgcagat ttttactcgg cggtgaacgc caccttccgc ttcattttgc
     6421 gaaagacggg catggaagga ctgcggcgct actgggcgga actcggccgc ggctattacg
     6481 cgccggtggc ggaccgctgg cggactggcg ggctgcctgc ggtggcggct tactggcgcg
     6541 ctttttttga cgcggagccc ggggctgtcg cggaggtgga gctgcggcgg gacgaggtgg
     6601 tcgtcgaggt cgggacatgc cccgccatcg gctacctgcg cgcgcacggc cgcgagatca
     6661 cgccctgctt ctgccagcac tgttattttg tcagcgaggc catcggccag ggggcgggcg
     6721 tcgaggtgcg tgtcagcggc ggtgacgggc ggtgcgtgca gcgcttcgcc aaagcggggc
     6781 atttcaccgc accgcaggca ttggaggaaa tccgcgcggc caccagtctc gggacggcgg
     6841 agacatcatc caccaactca aacgcatgaa cggcatcacg cggcacttct ggggctggga
     6901 cgcgcccgtg cttgagaagg cggcggcatt tttgaccagg gagagggagg cctcgcaagg
     6961 cgcgccggat ttccccggca tgctcctcat cgtgcccacc gccgaggcgg gcc
//
LOCUS       DAOMYJ010000253         6981 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Prosthecobacter sp. isolate AMR_MDS_5629
            NODE_3645_length_6981_cov_20.682212, whole genome shotgun sequence.
ACCESSION   DAOMYJ010000253 DAOMYJ010000000
VERSION     DAOMYJ010000253.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867850
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prosthecobacter sp. (wastewater metagenome)
  ORGANISM  Prosthecobacter sp.
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Verrucomicrobiaceae; Prosthecobacter.
REFERENCE   1  (bases 1 to 6981)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6981)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.30
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:34:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,742
            CDSs (total)                      :: 4,684
            Genes (coding)                    :: 4,676
            CDSs (with protein)               :: 4,676
            Genes (RNA)                       :: 58
            tRNAs                             :: 55
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6981
                     /organism="Prosthecobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3645_length_6981_cov_20.682212"
                     /isolate="AMR_MDS_5629"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1965333"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..810)
                     /locus_tag="PLP58_19220"
     CDS             complement(<1..810)
                     /locus_tag="PLP58_19220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome C peroxidase"
                     /protein_id="HRJ10720.1"
                     /translation="MKPAPRLLPKMPVLLLAAALACAAQAPDAALTLVFQPVAGNEPL
                     VLDAPRHRNAAGETWSVSRLSYLVSGVSLQRADGSWLGLPDAIAWMDAATRRDTLRIE
                     KIPAGPYQALRFHIGLDPLTNAADPASHPPEHPLNPNLNGLHWSWQGGYIFLVMEGLH
                     GTDPGRLTGYAFHLAREPFRTAVTLPCALDLRDDAALRVLFDTAAVLDAVKPVSFAKD
                     GATTHSKDGDPLALALRENLARAFRLPPDAFTAGVSASAPLPPAPTLPGPDM"
     gene            961..2262
                     /locus_tag="PLP58_19225"
     CDS             961..2262
                     /locus_tag="PLP58_19225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ10721.1"
                     /translation="MMKNPRSNFCPRTARAIGLAVLLLQGAGLRADVPVPPRVLEPLT
                     PAEAWNVIRLASANVERLIVEKRPLEVATQVSLCSPPLRVLSKASVTPEVQPVLDVLT
                     AKAFRAVNTTAKEGMADNLDGAAAAFQQLKLALGELARGFDPAVVEQEIHHCVAHPDV
                     ISTSPGKLCEVCRQPFVVRRIPYSFVYVRQDKPTVRLTLRPDSPLAAGKVCRVVARLE
                     TLEGQPVRPADLWVTHARHVHMLVVGPGLADFHDLEPAPTGQDGEYEFSFTPAFGGGY
                     RFRAGVVPAVTGLLEHPVADLACEGPEAPPPAGEETFSTTADGYRFSIALGTGRMGSP
                     KAERLEMLRVHVADAEGRPVTRLEPDRGAFAHFTGFYSDGKTVVQLHPVGGDILREDL
                     RGGPFLSFKFHPPHPGFMRLYCQVKVDGKPVRASFGMRIAE"
     gene            2295..2453
                     /locus_tag="PLP58_19230"
     CDS             2295..2453
                     /locus_tag="PLP58_19230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ10722.1"
                     /translation="MPELPKRSAPYIIILLLAVLLAITLWIHMAVNSPEVRQAVREKK
                     AAPALRRD"
     gene            2583..5429
                     /locus_tag="PLP58_19235"
     CDS             2583..5429
                     /locus_tag="PLP58_19235"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HRJ10723.1"
                     /translation="MRRPVRHLMILLLSLVMAGKGVGQQLEIPEGTPQELLQKGNAAY
                     MARDWPEAARVFQAFLDTYGSEAALAEAVTQVKPLLTLARIRLGEFGAAGELISECLT
                     FPRLEPVLKDELSFWKGIILLKVEAHEEARAAFLAYFQTPEFNALRKVEAILLYGTTF
                     VLEGKPEEAAAFFEQQSARLWELNRETALRAQTLRLHCLLETGQREKALDLVRTLQPY
                     MMEVTQLVSLHSLMAQLGGMLLEEGEFYPAIYCLQRVWAADRLMRHQAGRIRALNMEI
                     ESLLARPGTEPLVFQKRSVLTRVEREHKNFSADTDFDLGVRMRLGFAWLGLERWREAA
                     LVLEDALNLPGEPGQQAQAGMAVVQCWLTLERLDRCIAMADSWLLRFAGKAEADSEVK
                     VRFLMAQARHEDEQYQTAAAEFEALSKAHPKHELAPQALFMAGMGRLMADDYPEAMRI
                     FQEVRRVHDQQPVAEDADYWWGMALSFDKEYAACREHLAAHLKKHGKAARYHAQAVFR
                     RAYCLYALGDYEGALKEFAAYARDFPDGPDLAESRILTGDIYCSISEIDRGLESYRAV
                     SPDAGRWHDEAHFKTGNVLKLRKDWDGLRAHHQAYIAAKPESKRLPEAVYWAGHASIA
                     QERLDEAREQYWQALITYGDKAGHHGIEDILLALPRLYRGETGRVELLREIQRRRAEA
                     DKAGQKTLVSRLHWMEGHMQPADKPRLAQADFMMAASLLDVEKQNPRVIADCADASFA
                     AGSKSRARELYTDLIRWHPRAVEVDRALAGLGFLAAEAGDALEALAWFERFEKRVSTM
                     TLRPRVLMKKAELLASGKKPDQAVEVYKALLEDKLTPARAKAEALLAWAALWEKQGQV
                     LKATALYERVYLSYGRSRDLVAQAYLARGKALEKLGKKLEAAEVYDELVKTEMLRDYP
                     EHAEAAKRLQPLQPLLDEARLRQAEAEKEKEVKP"
     gene            5555..6457
                     /locus_tag="PLP58_19240"
     CDS             5555..6457
                     /locus_tag="PLP58_19240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ10724.1"
                     /translation="MPHLAFAALLGLASLHAQDVLHLQDGRKLRGKVQRLASQAIAML
                     EVLPGGRGSAQRDIPPDQVAFIDFAPLPGEAEALANPANAAMEKRLLELWQEKSVNLR
                     WPSNNAGLIGLTLAGQSMKSGDSLLIERAFRIYSLIEKEDWDEKRRDEAKRGRLRCLI
                     QLKRIDEAIAEAQKLAESDEEPGLLLEAGLVLAQADFERLKIFEKDHPRWMEDDELAA
                     ARTKLYHQTLDQFLQAPLFHGSMEDKAAESLWGAVQVHLFAKENRAALDRARDLLQLY
                     PKTAQAAEARKLLPSETPAPSPDQ"
     gene            6463..>6981
                     /locus_tag="PLP58_19245"
     CDS             6463..>6981
                     /locus_tag="PLP58_19245"
                     /inference="COORDINATES: protein motif:HMM:NF013760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HRJ10725.1"
                     /translation="MKLDSIIPQLGLRSLLLALALSAAPAFAQDPAPPAPEVVDTTSL
                     LDLYHQGGWTMHILLVFSVGTVAVAAYCFLNVSGKKMAPKSMVDALTHSMQKKDVGAA
                     YQYCLANPCSITRVLSSALLKVNFDRDQANKVSMEEAAGEALDAEETRYTMWVNYLNV
                     FATISPMIGLFGT"
BASE COUNT         1189 a         2281 c         2397 g         1114 t
ORIGIN      
        1 catgtccggg ccgggcaggg ttggcgcggg cggcaggggc gcggaggcgg agacgcccgc
       61 cgtgaaggca tcgggcggaa ggcggaaggc gcgggccaga ttttcccgca gggccagggc
      121 cagcgggtcg ccgtccttgg agtgggtggt ggcgccgtct ttggcgaagg agacgggctt
      181 cacggcatcg agcacggcgg cggtgtcaaa gagcacgcgc agggcggcat catcccgcag
      241 atccagcgcg cagggcaggg tgacggcggt gcggaagggt tcgcgcgcca gatggaaggc
      301 gtagccggtg agcctgccgg ggtccgtgcc gtgcagtcct tccatcacca gaaagatgta
      361 gccgccctgc cagctccagt gcaggccgtt gaggttggga ttcaaggggt gctccggcgg
      421 gtgggaggcg gggtcggcgg cgttggtcag cgggtcgagg ccgatgtgga agcgcagcgc
      481 ctggtaaggc ccggcgggga ttttctcaat gcgcagggtg tcgcggcggg tggcggcatc
      541 catccatgca atggcatccg gcaggccgag ccatgagccg tccgcgcgct ggagggagac
      601 gccggagacc aggtagctga ggcgggagac ggaccaggtc tcgcccgccg cattgcggtg
      661 gcgcggcgcg tcgagcacaa gcggctcatt tccggccacg ggttgaaaga ccagagtcag
      721 cgcggcatcc ggtgcctgcg cggcgcaggc gagcgccgct gcgagaagca ggacgggcat
      781 ctttggcaaa agacgaggcg cgggtttcat gactgctcgt ggaggccccg gtggattatt
      841 cgggcaatta ttcgagcagc gcgcactatc ctccgaacag cgctccaaag gcaagcttgg
      901 aatcacagga gagatgttga ttgcggacgg ggcggcggag gtgatagcct gcgcggcgtg
      961 atgatgaaaa atccccgatc gaatttttgc ccgcgcactg cccgcgccat cgggctggct
     1021 gtcctgctgc tgcaaggggc cgggctgcgc gcggacgtgc cggtgccgcc gcgagtgctg
     1081 gagccgctga cgccggcgga ggcatggaat gtgatccggc tggcctccgc caatgttgag
     1141 cgcctgatcg tggagaagcg tcctctggag gtcgccacgc aggtctccct gtgcagtccg
     1201 ccgctgcgcg tgctgtccaa agccagcgtc acccccgagg tgcagcccgt gctggatgtg
     1261 ctgaccgcga aagccttccg cgcggtgaac acgaccgcca aggagggcat ggcggacaat
     1321 ctggacggcg ccgccgccgc ttttcagcag ttgaagctgg ccctgggtga attggcgcgc
     1381 ggctttgatc cggcggtggt ggagcaggaa atccatcatt gtgtggcgca tcccgacgtc
     1441 atctccacaa gtccgggaaa actctgcgag gtctgccggc agcccttcgt cgtccggcgc
     1501 atcccgtaca gttttgtcta tgtcaggcag gacaagccca cggtgaggct caccctccgg
     1561 ccggactctc cactggcggc gggcaaggtg tgcagggtgg tcgcgcggct ggagacactg
     1621 gagggacagc cggtgcggcc cgccgacttg tgggtcactc acgcgcggca tgtccacatg
     1681 ctcgttgtgg ggccgggact tgccgacttc cacgatctgg aacccgcgcc caccggccag
     1741 gatggggagt atgagttcag cttcaccccc gcgttcgggg gcggctaccg gttccgcgcc
     1801 ggagtcgtgc ccgcggtcac cgggctgctg gagcatcccg tcgcggacct ggcctgcgag
     1861 gggccggaag cgccgccgcc tgccggggag gagacctttt ccaccacggc ggacggctac
     1921 cgtttcagca tcgcgctggg caccggcagg atgggatcgc ccaaggcgga gcgcctggag
     1981 atgctgcgcg tccatgtcgc cgatgctgag ggcaggccgg tgacccggct ggagccggac
     2041 cggggcgcct tcgcgcactt cacgggcttt tactccgacg gcaaaacggt ggtgcaactg
     2101 catcccgtcg gcggcgacat cctgcgcgag gacctgcgcg gcggtccgtt tctcagcttc
     2161 aaatttcatc cgccgcatcc cggtttcatg cgcctttact gccaggtcaa agtggatggc
     2221 aaacccgtgc gcgcctcctt cggcatgagg attgccgagt gaccggctgg ccggaaaaga
     2281 acatccccaa cgtcatgccc gagctgccga aacgctccgc gccttacatc atcatcctgc
     2341 tgcttgccgt gctgctggcg atcactctct ggattcacat ggccgtgaac tcgccggagg
     2401 tgcggcaggc cgtgcgggag aaaaaagccg ccccggcctt gaggcgggac tgacgctttt
     2461 cacctccatg ccatgcccgc cggaacattc cggctggggc aattcgatct ggatgccgcg
     2521 ggtgacaaaa tctccgctgg ccttttccct gctgtgtcgt tacaccctca cgctgtccgc
     2581 ccatgcgccg tccggtccgc catttgatga tcctgttgct gtccctggtg atggcgggga
     2641 agggtgtggg gcagcagctg gagatcccgg agggcacgcc gcaggagctg ctgcaaaagg
     2701 ggaacgcggc ctacatggcg cgggactggc cggaggcggc gcgggttttc caggcctttc
     2761 tggacaccta cggctcggag gcggcgctgg cggaggcggt gacgcaggtg aagccgctgc
     2821 tgacgctggc gcgcatccgc ctgggggagt tcggcgcggc gggggagctg atctcggagt
     2881 gcctcacctt tcccaggctg gagccggtgc tgaaggacga gctgtctttc tggaaaggca
     2941 tcatcctgct gaaggtggag gcgcatgagg aggcgcgcgc ggctttcctg gcctacttcc
     3001 agacgccgga gttcaacgcg ctgcgcaagg tggaggccat tttgctctac ggcaccacct
     3061 ttgtgctgga ggggaagccg gaggaggcgg cggccttttt cgagcagcag tcggcgcggc
     3121 tatgggagct gaaccgggag acggcgctgc gcgcgcagac gctgcgcctg cactgcctgc
     3181 tggagacggg ccaacgggag aaggcgctgg acctggtgcg cacgctccag ccttacatga
     3241 tggaggtgac gcagctcgtg tccctgcaca gcctcatggc gcagctcggc ggcatgctgc
     3301 tggaggaggg ggagttttac cccgccatct actgcctcca gcgcgtgtgg gcggcggacc
     3361 ggctgatgcg ccaccaggcg gggcgcatcc gcgcgctgaa catggagatc gagtccctgc
     3421 tggcgcgccc cggcacggag ccgctggtct tccagaagcg cagcgtgctg acccgcgtgg
     3481 agcgggagca caagaacttc tcggccgaca cggacttcga cctcggcgtg cgcatgaggc
     3541 tgggcttcgc ctggctcggg ctggagcgct ggcgagaggc cgccctggtg ctggaggacg
     3601 cgctgaacct gcccggcgag ccggggcagc aggcgcaggc gggcatggcc gtggtgcagt
     3661 gctggctgac gctggagcgg ctggaccgct gcatcgccat ggcggactcg tggctgctgc
     3721 gcttcgcggg caaggcggag gcggacagcg aggtgaaggt gcgcttcctc atggcgcagg
     3781 cccggcacga ggatgagcaa taccagacgg cggcggcgga gttcgaggcg ttgagcaagg
     3841 cgcatccgaa gcacgagctg gctccgcagg cgcttttcat ggcgggcatg ggccggctga
     3901 tggcggacga ttacccggag gcgatgcgca tttttcaaga ggtgcgccgc gttcatgacc
     3961 agcagccggt ggcggaggac gcggactatt ggtggggcat ggcgctgagc ttcgacaagg
     4021 agtatgccgc ctgccgcgag catctggccg cgcatttgaa gaagcatggc aaggccgcgc
     4081 gctaccatgc gcaggccgtc ttccgccgcg cctactgcct gtatgcgctg ggggactatg
     4141 agggcgcgct gaaggagttc gccgcctatg cgcgcgactt ccccgacggg ccggacctgg
     4201 ccgagagccg gattttgacg ggggacatct actgctcgat cagcgagata gaccgcggcc
     4261 tggaaagcta ccgcgccgtg tcacccgacg cgggccgctg gcatgacgag gcgcatttta
     4321 aaaccggcaa tgtgctcaag ctgcgcaagg actgggacgg cctgcgcgcg caccatcagg
     4381 catacatcgc cgccaagccg gaaagcaagc ggctgccaga ggcggtgtat tgggcgggcc
     4441 atgccagcat cgcccaggag cggctggacg aggcgcggga gcagtattgg caggcgctca
     4501 tcacttatgg cgacaaggca gggcaccacg gcatcgagga catcctgctc gccctgccgc
     4561 gcctctaccg tggcgagacc gggcgcgtgg aactgctgcg ggagatccag cgccgccgcg
     4621 ccgaggcgga caaggccggg cagaagaccc tggtcagccg tctgcactgg atggagggcc
     4681 acatgcagcc ggcggacaaa ccccgcctgg cgcaggccga cttcatgatg gccgcctccc
     4741 tgctcgatgt ggagaagcag aacccgcgcg tcatcgccga ctgcgccgac gcctcctttg
     4801 ccgcaggcag caagagccgc gcgcgcgaac tctacaccga cctcatccgc tggcaccccc
     4861 gcgccgtgga ggtggaccgc gccctggccg gcctcggctt cctggccgcc gaagctgggg
     4921 atgcgctcga ggcgctggcg tggttcgagc gcttcgagaa gcgcgtctcc accatgaccc
     4981 tgcgcccgcg cgtgctcatg aagaaggccg aacttctcgc ctccgggaaa aagcccgacc
     5041 aggccgtgga ggtgtacaaa gccctgctcg aagacaagct cacacccgcc cgcgccaagg
     5101 ccgaggcctt gctcgcctgg gccgccctct gggaaaagca ggggcaggtg ctcaaagcca
     5161 ccgccctcta cgagcgcgtc tatctcagct acggccgcag ccgcgacctc gtggcccagg
     5221 cctacctcgc ccgcggcaag gcgctggaaa agctcggcaa gaaactcgaa gccgccgaag
     5281 tctatgacga actcgtgaag acggagatgc tccgggatta ccccgagcac gccgaagccg
     5341 ccaagcgcct gcaaccgctc cagcctttgc tcgatgaagc ccgcctgcgc caagccgagg
     5401 cagagaagga gaaggaggtg aaaccatgat ctgccgccct ccatctcgta atccgtgtct
     5461 tgatccttgt cgtaatccaa gcgcgccgga ggcgtgttcg gcggggatga agacacggat
     5521 taagacaagg attacgagtc agagattcat cacggtgcct cacctcgcat ttgccgcgct
     5581 gcttggcctc gccagcctcc acgcccagga cgtgctccac ctccaggacg ggcgcaaact
     5641 gcgcggcaag gtgcagcgcc tcgcctccca ggccatcgcc atgcttgagg tgctgcccgg
     5701 cgggcgcggc tccgcgcagc gcgacatccc tcccgaccag gtggccttca ttgacttcgc
     5761 cccgctgccc ggcgaggccg aggcactcgc gaaccccgcc aatgccgcca tggaaaagcg
     5821 cctgctggag ctgtggcagg agaagtccgt caacctacgc tggccctcga acaacgccgg
     5881 cctcatcggc ctcaccctcg ccggccagtc catgaagtcg ggtgactccc tgctcattga
     5941 gcgcgccttt cgcatctact ccctcatcga aaaagaggac tgggacgaaa agcggcggga
     6001 tgaggccaag cgcggccgcc tgcgctgcct catccagctc aagcgcattg atgaggccat
     6061 cgccgaggcg caaaagctgg ccgagtccga cgaggagccc gggctgctgc tggaggccgg
     6121 tcttgtgctc gcgcaggcgg acttcgagcg gctgaagatt ttcgaaaaag accatccgcg
     6181 ctggatggag gacgacgagc tggccgccgc ccgcaccaag ctctaccacc agacgctcga
     6241 tcaatttctc caagccccgc tcttccacgg ctccatggag gacaaggccg ccgagtccct
     6301 ctggggcgcc gtgcaggtgc atctcttcgc caaagaaaac cgcgccgcgc tcgaccgcgc
     6361 gcgcgatctg ctccagctct acccgaaaac cgcccaggcc gccgaagccc gcaaactcct
     6421 tccctcagaa acccccgccc cctctcccga ccagtgaccc ttatgaaact cgactcgatc
     6481 atcccgcagc ttggcctccg ctctctgctc ctggccctcg ctctctccgc cgcgcccgcc
     6541 tttgcccagg accccgcccc gcccgcgccg gaagtcgtgg acaccacctc gctgctggac
     6601 ctctaccacc agggcggctg gaccatgcac atcctgctcg tcttctccgt cggcaccgtg
     6661 gctgtggccg cgtattgctt cctcaacgtc agcggcaaaa agatggcgcc aaagagcatg
     6721 gtggacgcgc tcacccactc catgcagaaa aaggatgtcg gcgccgctta tcaatactgc
     6781 ctggccaacc cgtgcagcat cacccgcgtg ctttcctccg cgctgctgaa ggtgaacttc
     6841 gaccgcgacc aggccaacaa agtctccatg gaggaggcag ccggcgaggc gctcgacgcc
     6901 gaggagacgc gctacaccat gtgggtgaac tacctcaatg tcttcgccac catctcgccc
     6961 atgatcggcc tcttcggcac c
//
LOCUS       DAOMYJ010000254         6971 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Prosthecobacter sp. isolate AMR_MDS_5629
            NODE_3654_length_6971_cov_21.004916, whole genome shotgun sequence.
ACCESSION   DAOMYJ010000254 DAOMYJ010000000
VERSION     DAOMYJ010000254.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867850
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prosthecobacter sp. (wastewater metagenome)
  ORGANISM  Prosthecobacter sp.
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Verrucomicrobiaceae; Prosthecobacter.
REFERENCE   1  (bases 1 to 6971)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6971)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.30
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:34:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,742
            CDSs (total)                      :: 4,684
            Genes (coding)                    :: 4,676
            CDSs (with protein)               :: 4,676
            Genes (RNA)                       :: 58
            tRNAs                             :: 55
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6971
                     /organism="Prosthecobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3654_length_6971_cov_21.004916"
                     /isolate="AMR_MDS_5629"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1965333"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..367)
                     /locus_tag="PLP58_19250"
     CDS             complement(<1..367)
                     /locus_tag="PLP58_19250"
                     /inference="COORDINATES: protein motif:HMM:TIGR02226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BatA domain-containing protein"
                     /protein_id="HRJ10726.1"
                     /translation="MSLIPASLTFANPLGLWALLGLPVVVAIHFLQRRQRRVTCTTLF
                     LLEQMRRESRTGNRFEKLRVSIPFWLQLLMVLLLAWLLAQPRWLGRDAVQRVAVVLDA
                     SASMAALASLPNGKVSFHPD"
     gene            complement(406..1290)
                     /locus_tag="PLP58_19255"
     CDS             complement(406..1290)
                     /locus_tag="PLP58_19255"
                     /inference="COORDINATES: protein motif:HMM:NF013998.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HRJ10727.1"
                     /translation="MSLLHDTAALRALHAQARGWARVLMLPFRQQRWRGQAGSFLGTG
                     TGSSLDFHDHRAYLPGDDPRQINWQAYARTGSYTMKLYREEVRPLVDVILDVSASMFA
                     HAAKERRALELLAFVCESALQSGASLRCHAVAGAECRALEPESILGGQWPERVAGMSA
                     VSAPPDLQRVQLRAGALRVLVSDLLFTCPPEAVLGSLAGRQGRGIVLAPFAREEADPA
                     WDGSYEFLDPETGLPHDHRVEPALLRRYEAAYRRHFDLWKAAALRHHIALVRVSSDAE
                     FLKALQTEGVPAGAVEMG"
     gene            complement(1274..2710)
                     /locus_tag="PLP58_19260"
     CDS             complement(1274..2710)
                     /locus_tag="PLP58_19260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ10728.1"
                     /translation="MVKELRQGLRTRFFTYALTLFHTALILLLACANLELSLEMMNSA
                     FWVLTGIMLLVVLPLRGFDALHSEAKAGTLDMLALTSMPAQRILQGKWAAIYGQTLLV
                     ACSVLPYMVARYFFGGVEIAREALGLASLVLGSGILSAALLAFSSQASLLLRLTLGVA
                     LGFAALPLGFFTAGVVMSTEADSLLMDFRDLSLWQQAGIVVLVLTLTVYSIWYLLAMG
                     ASRIAPASENHSTRKRFAALVMLSLLTLAGVLHSLLTNDAEELIWYFLPLLFLTMFTC
                     MDVMTEEMPHFPTCVVGLQRLRNLSGIFYRLFLPGWASGVAFSMLLCLLTLAFTMAVI
                     LCRAGLMGDLDWDVPQGITCFLAAPFLPVLIQVSKANHFANWWVVQIILLALGFGLVI
                     SGEVLDVDWLPGLSFVTPTTAHFGVQSIAYEYRDTVLVLSASLSGIWMLAALVYAWRF
                     FPLYRELEDQALSLDKPPPPAADDVPAA"
     gene            complement(2767..4209)
                     /locus_tag="PLP58_19265"
     CDS             complement(2767..4209)
                     /locus_tag="PLP58_19265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ10729.1"
                     /translation="MTQTLTLSDFPDRLSPMVVKELRQGMRTRAFAIVMLTLHLVLVL
                     LTLLGGGGGNSEIAHAWFDGIVTLVLCIILPLRGFSAVADEIRAGTLDMLALTRLSPW
                     RIVFGKWLSIAAQSLLIALSILPYVVARYVHGGLDLFGEMLALGGKTLAGLVLAAFIV
                     ALSTQRNFWLRLLVVGLPMLMGGCGMMSYMMMSRVGGMSPGTRLSISGSFSAFTADGV
                     TGVLLVLAYAVWAVFYFLSLAATRIAPAASLLSVPKRLVHLAALAVACVIGWSQGNAM
                     AAGGLIGFIMLDVLTEPVNTVPSVYAAFYRRGLPGRLGMWLLAPGWVTGFLFTVLLTA
                     GTAALAFSTGGPELAMLVFITACGTWMVAALVQLLPRARQGADLFPAFLLWWVVLSVL
                     LSWLGGLLVMQSTMTSTTPWFTCALPQMVPLGHAIAQAADKAAFLRMATLAALVWPLV
                     LLVPAAFAWRRAREARREARMMTRPGGTEA"
     gene            complement(4229..4657)
                     /locus_tag="PLP58_19270"
     CDS             complement(4229..4657)
                     /locus_tag="PLP58_19270"
                     /inference="COORDINATES: protein motif:HMM:NF023580.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3592 domain-containing protein"
                     /protein_id="HRJ10730.1"
                     /translation="MNLIATACHLLGLFLLFVSRDSIRLGWQSRRWPHTQGIVTHISD
                     KSYTLDTVTRQGPRIVHHSCWLPHYRYEVDGVEYESTGVCFGGRLDEEKARFKQGGRV
                     SVYYDPQNPANAVLLPSVQPTSLAGLAPIGAGLLVGFFGG"
     gene            complement(4903..5925)
                     /locus_tag="PLP58_19275"
     CDS             complement(4903..5925)
                     /locus_tag="PLP58_19275"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRJ10731.1"
                     /translation="MPTTSTHEMDRAQPAVLVEDLHRNFGELKAVDGVSFTIDHGRVT
                     GFVGANGAGKTTTMRIMATLDSPGRGSVRIGGHNVVHHPAKVRRLLGWMPDSFGAYEN
                     MTVLEYMDFYARALGFKGGERARRVQEVMEFTDLAPLASRMCDKMSKGQTQRLCLGRA
                     LLHDPQILILDEPAAGLDPKARVELKNLIRILAQEGKTILISSHILSELAEMCDSLLF
                     IDKGRIVHHGDAESMIRGTESAGATLVNVQVAGDPALLHQWALTAPLIQIVEETKRGA
                     RLRIQAEDEATLAGVLRRMIQDGLPVLDYHREERRLEDAFIDMLGRLDSGAPPPPLPL
                     IPPKSA"
     gene            complement(5968..>6971)
                     /locus_tag="PLP58_19280"
     CDS             complement(5968..>6971)
                     /locus_tag="PLP58_19280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ10732.1"
                     /translation="GEISVFGWDGTKLAALETVNRYWGEPARLKKLVERHSTASDWPL
                     LGLLGERSFASWQVIAFLVIFGILVGPVNLFVLAPSGRRHRLFLTTPLLSLGASVIMV
                     VLILVQDGTGGTGRRFVHLHLEPDDAAAFVTQEQVCRTGVLLGAAFETAQPATIEPLA
                     LPDTQWVKLKSTHNTQAVQLTQNGRERRGNYFQSRTEQGHLLRAVVPTRARLELKAGT
                     APGAAPEVISALGFTVDWLCYKDDERQWWVLEQPLATGASAVLKKAEAVAVQKTLGDA
                     AKPAEQTLRDKLTNHFIERTGWFYARASQAPGLTLDTLAAIRWQDDRILVSGPVVKP"
BASE COUNT         1374 a         2230 c         2278 g         1089 t
ORIGIN      
        1 gatcggggtg gaaagaaact ttcccgttcg gcagcgaagc caaggccgcc atgctggccg
       61 aggcatccag caccacggcc acgcgctgca ccgcgtccct ccccagccag cgcggctggg
      121 ccagcagcca ggccagcagc agcaccatga gaagctgaag ccagaagggg atggagacgc
      181 gcagcttttc aaagcggttc cccgtgcggc tctcccgccg catttgctcc aacagaaaca
      241 gggtcgtgca tgtcacgcgc cgctgccgcc gctgaagaaa atgaatcgcc accaccaccg
      301 gcagccccag caatgcccaa agcccgagag ggttggcaaa ggtgaggctg gcggggatga
      361 gggacatgtt gggaggatca aaggttgtca gcgtgattcc ctgcctcacc ccatttccac
      421 ggcgccggcg ggcacgcctt cggtttgcag tgctttgaga aactccgcgt ccgaggacac
      481 gcggaccagg gcgatgtggt gcctgagggc ggcggctttc cagagatcaa aatggcggcg
      541 gtaggcggcc tcgtagcggc gcagcagcgc gggctccacg cggtggtcgt gcggcaggcc
      601 ggtctcggga tcgagaaact cgtagctgcc atcccaggcg ggatcggcct cctcccgcgc
      661 gaagggcgcc agcacgatgc cgcgtccctg gcgtccggcc aggctgccga gcaccgcctc
      721 gggcgggcag gtgaaaagca gatcactcac cagcacgcgg agcgctcccg cgcgcaactg
      781 cacgcgctgg agatcgggcg gcgcggagac cgcgctcatg cccgccaccc gctccggcca
      841 ttgcccgccg aggatgctct ccggctccag cgcgcggcat tccgcgccag ccacggcgtg
      901 gcagcgcagg gaggcgccgc tttgcagcgc ggactcgcag acaaaggcca gcagctccag
      961 cgcgcggcgt tctttggccg cgtgggcgaa catggaggcg gacacgtcca gaatcacatc
     1021 caccagcggg cgcacctcct cgcggtagag cttcatcgtg taggagccgg tgcgcgcata
     1081 ggcctgccag ttgatctggc gcggatcatc ccccggcagg taggcgcggt gatcgtggaa
     1141 atccaggctg ctgcccgtgc cggtgccgag aaaggagccc gcctgcccgc gccagcgctg
     1201 ctggcggaag ggcagcatca gcacccgcgc ccacccacgg gcctgcgcgt gcagcgcgcg
     1261 caaggcggcg gtgtcatgca gcagggacat cgtcagcagc aggtggcggg ggtttgtcca
     1321 ggctcagggc ctggtcttcg agttcgcggt agagggggaa aaatctccat gcatacacca
     1381 gcgcggcaag catccagatg ccactcaagc tggcactgag gaccagcacc gtgtctcgat
     1441 attcataagc gatggattgc actccaaagt gcgcggtcgt tggtgtcaca aagctgagtc
     1501 caggcagcca gtccacatca agcacttctc cgcttatcac aaggccaaag ccaagtgcca
     1561 aaagaatgat ctgcaccacc caccaattgg cgaagtggtt tgctttgctc acctgtatca
     1621 acacaggaag gaaaggcgca gccaggaagc aggttattcc ttgtggcaca tcccagtcta
     1681 aatctcccat taagccagcc ctgcagagta taaccgccat ggtgaacgcc aaagtcagca
     1741 ggcaaagaag cattgagaat gcgactcctg atgcccatcc aggaagaaag agccgataaa
     1801 atatcccact caagttccgc agtctctgaa gtcccacaac gcaggtggga aagtgtggca
     1861 tttcctcggt catcacgtcc atgcaggtga acatcgtcag aaacaaaagg ggcagaaaat
     1921 accagattag ttcttcggca tcattcgtca gcaaggaatg aagcaccccg gccaatgtca
     1981 gcagacttag catgaccagc gcggcgaagc gcttgcgtgt gctgtgattt tcggaagcgg
     2041 gagcaatgcg tgaggcgccc atcgccagca gataccagat gctgtaaacg gtcagcgtca
     2101 gcaccagcac aacgattccc gcctgctgcc acaggctcag atctctgaaa tccatgagga
     2161 ggctgtccgc ctcggttgac atcaccaccc cggctgtaaa aaagcccagc ggcagcgccg
     2221 cgaaccccag cgccaccccc agtgtcagcc gcagcagcag cgacgcctgc gaggaaaagg
     2281 ccagcaaggc ggcggagaga atgccggagc cgagcacgag agaggcgagc cccagcgcct
     2341 cgcgcgcgat ctccaccccg ccgaaaaaat aacgcgccac catgtagggc agcaccgagc
     2401 acgccaccag cagcgtctgg ccgtaaatgg cagcccattt cccctggagg atgcgctgcg
     2461 cgggcatgga ggtcagcgcc agcatgtcca gcgtgcccgc cttggcctcg ctgtgcagcg
     2521 cgtcgaagcc gcgcagaggc aggacgacca gaagcataat accggtgaga acccagaagg
     2581 cggagttcat catctccagc gacagttcca gattcgcaca ggccagcagc agaatgagcg
     2641 ccgtgtggaa aagcgtgagc gcgtaagtga agaagcgcgt gcgcagcccc tggcgcagct
     2701 ccttgaccac gatggggctg aagtggtcgc caaaatcctg cggcaatgcg agcgctggcg
     2761 gtgggttcat gcttcggttc ctccgggtct tgtcatcatc cgtgcctcgc gccgcgcctc
     2821 ccgcgcgcgt ctccatgcga aggcggccgg caccagcagc acgagcggcc acaccagcgc
     2881 ggccagggtg gccatgcgca aaaacgccgc cttgtccgcc gcctgagcga tggcgtggcc
     2941 cagcggcacc atctgcggca gcgcgcaggt gaaccagggc gtggtgctcg tcatcgtgct
     3001 ctgcatgacc agcagaccgc ccagccatga cagcagcacg gacaaaacca cccaccagag
     3061 caggaaggcg gggaacaaat ccgcgccctg gcgcgcgcgc ggcaggagct gcaccagcgc
     3121 cgccaccatc caggtgccgc aggcggtgat gaagaccagc atggccagct ccggcccgcc
     3181 cgtcgaaaac gcgagcgccg ccgtccctgc tgtcagcagc acggtgaaaa ggaaccccgt
     3241 cacccagccg ggtgccagca gccacatccc cagcctgccc ggcagaccgc gccggtaaaa
     3301 agccgcgtac actgagggca ccgtgttcac cggctcggtc agcacatcca gcatgatgaa
     3361 accgatcagc ccgccggcgg ccatcgcatt gccctggctc cacccgatga cgcaggcaac
     3421 cgccagcgcc gccagatgca ccagcctttt cggcaccgag agcagcgatg ccgcaggtgc
     3481 gatgcgcgtg gcggccaggc tcaggaaata aaacaccgcc cagacagcat aggcgaggac
     3541 gagcagcact cccgtcactc catcggcggt gaaagcggag aatgaaccgg agatggagag
     3601 gcgcgtgccc ggactcatcc ccccaacgcg ggacatcatc atgtacgaca tcatcccgca
     3661 cccgcccatg agcatcggca gtcccaccac gagcaggcgc agccagaagt tccgttgcgt
     3721 ggacaaagcc acgatgaagg ccgcaagcac gagtcccgcc agagtttttc ctcccagcgc
     3781 cagcatctcc ccgaacaagt ccagcccgcc atgcacatag cgcgccacca catagggcag
     3841 gatgctcagg gcgatgagca gcgactgcgc ggcgatggac agccacttgc cgaagacaat
     3901 gcgccacggc gacagccgcg tgagcgccag catgtccagc gtgcccgccc tgatctcatc
     3961 cgccacggcg gaaaagccgc gcaaaggaag gatgatgcaa agcacgagtg tgacgatgcc
     4021 gtcgaaccac gcatgggcga tctctgaatt gccgccacct ccgcccagca gcgtgagcag
     4081 caccagcacg agatgcagcg tcagcatgac gatggcaaag gcgcgcgtcc gcatcccctg
     4141 ccgcagctct ttgaccacca tgggcgaaag gcggtccggg aaatcggaga gggtgagggt
     4201 ttgtgtcatg cgcttggggt tttgatcatc agcctccaaa gaatccaacc agcagcccgg
     4261 ccccgatggg agcgagtccg gccaaacttg tgggctggac actgggcagc aggaccgcgt
     4321 tggcgggatt ctgcggatcg taataaacac tcactctccc gccttgcttg aagcgcgctt
     4381 tctcttcatc cagcctgccg ccaaagcaca ccccggtgga ttcatactcc acgccatcca
     4441 cctcatagcg gtagtgagga agccagcatg aatgatgcac gatgcgcggc ccctgtcggg
     4501 tgaccgtgtc cagcgtgtag cttttatcag aaatatgcgt gacgatgccc tgggtgtgcg
     4561 gccacctccg ggattgccag cccagccgga tggagtctct gctgacaaag agcaggaaca
     4621 atcccaaaag atggcaggct gtggcgatca ggttcatgat ttcagcaaac agagatgatt
     4681 cccgcactct ggcgcgcgcc tgggtagggc ggctggtcct ccagccgccg cgagcgaagg
     4741 cgcagggtac gaatggcgtg gaagggtggc tcggaggtct ggcttgcgct cgcggcgcgt
     4801 tgaggacaac gcgccctacc tggtgccgca ggcgcgcttg ccaaaacgcg ggagacggct
     4861 gcgagagcag ggcaggtttc ttcgatgaca aggcgcatgg tgtcaggcgg actttggtgg
     4921 aatgagtggc agtggcggag gcggcgcgcc ggagtcgagg cggccgagca tgtcaatgaa
     4981 ggcgtcctcc aggcggcgct cctcgcggtg gtagtcgagc acgggcaggc cgtcctggat
     5041 catgcggcgc agcacgccgg cgagggtggc ctcgtcctcc gcctggatgc gcaggcgcgc
     5101 gccgcgcttg gtctcctcga cgatctggat gagcggcgcg gtgagcgccc attggtggag
     5161 cagcgcaggg tctccggcca cctgcacatt caccagcgtg gcgcccgcgc tttcggtgcc
     5221 gcggatcatg ctttcggcgt cgccatggtg gacgatgcgg cctttgtcta tgaagagcag
     5281 gctgtcgcac atctccgcca gctcgctgag gatgtgggag ctgatgagga tggtcttgcc
     5341 ctcctgggcg aggatgcgga tgaggttttt cagctccacg cgcgccttcg ggtccaggcc
     5401 tgcggcgggc tcgtcgagaa tgaggatctg cgggtcgtgc agcagggcgc ggcccaggca
     5461 gaggcgctgg gtctggcctt tcgacatctt gtcgcacatg cgcgaggcca gcggggcgag
     5521 gtcggtgaac tccatgacct cctgcacacg gcgtgcgcgc tccccgcctt tgaagccgag
     5581 cgcgcgggcg tagaagtcca tgtattcgag cacggtcatg ttttcatacg cgccgaagct
     5641 gtccggcatc cagccgagca gccggcgcac tttggccgga tgatgcacca cattgtgccc
     5701 gccgatgcgc acgctgccgc gccccggcga gtcgagcgtg gccatgatgc gcatggtggt
     5761 ggtcttgccc gcgccgttgg cgccgacaaa gccggtcacg cgcccgtgat caatggtaaa
     5821 ggacactccg tccacagcct tcagctcccc aaaattgcgg tgcagatcct cgacgagcac
     5881 cgctggctgg gcgcggtcca tttcatgggt ggaggtggtg ggcatgattc cgtttttgcg
     5941 tctttttgtt ttttttgcgg ccaatcctca cggcttcacc accggcccgg agacgaggat
     6001 gcggtcgtcc tgccagcgga tggcggcaag ggtgtcgagg gtgaggccgg gggcctggct
     6061 ggcgcgggcg taaaaccagc cggtgcgctc gatgaagtgg ttggtgagct tgtcgcgcag
     6121 agtctgctcg gcgggctttg cggcatctcc cagcgttttt tggacggcca cagcctccgc
     6181 ctttttcagc acggcgctgg cgccggtggc gaggggctgc tccagcaccc accactggcg
     6241 ctcgtcatcc ttgtagcaaa gccagtccac ggtgaagccg agagcggaga tgacctcggg
     6301 cgcggctccg ggcgcggtgc cggctttgag ttccaaacgc gcgcgcgtgg gcacgacggc
     6361 gcgcagcagg tgcccctgct cggtgcggct ttgaaagtaa ttgccgcgcc gctcgcgccc
     6421 gttctgggtg agctgcacgg cctgggtgtt gtgggtgctt ttgagcttca cccactgggt
     6481 gtccggcaag gccagcggct cgatggtggc gggctgcgcg gtttcaaaag ccgcgcccag
     6541 cagcacaccc gtgcggcaga cctgctcctg cgtgacgaag gcggcggcat cgtccggctc
     6601 caggtgcagg tgtacaaagc gcctgccggt gccgccggtg ccatcctgca ccaaaatgag
     6661 caccaccatg atgacgctgg cgccgaggga gagcagcggc gtggtgagga aaagccggtg
     6721 ccgccggccg ctgggggcta gcacgaaaag attcaccggc ccgacgagga tgccgaagat
     6781 gacgagaaag gcgatgacct gccaggaggc aaaactgcgc tcgccaagca gaccgagcaa
     6841 tggccagtcc gaggcggtgc tgtgcctctc gacgagtttt ttcagccgcg cgggctcgcc
     6901 ccagtaacgg ttcacggttt cgagggcggc gagtttcgtg ccgtcccagc cgaagacgct
     6961 gatctcgccg a
//
LOCUS       DAOMYJ010000255         6962 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Prosthecobacter sp. isolate AMR_MDS_5629
            NODE_3661_length_6962_cov_22.143333, whole genome shotgun sequence.
ACCESSION   DAOMYJ010000255 DAOMYJ010000000
VERSION     DAOMYJ010000255.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867850
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prosthecobacter sp. (wastewater metagenome)
  ORGANISM  Prosthecobacter sp.
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Verrucomicrobiaceae; Prosthecobacter.
REFERENCE   1  (bases 1 to 6962)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6962)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.30
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:34:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,742
            CDSs (total)                      :: 4,684
            Genes (coding)                    :: 4,676
            CDSs (with protein)               :: 4,676
            Genes (RNA)                       :: 58
            tRNAs                             :: 55
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6962
                     /organism="Prosthecobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3661_length_6962_cov_22.143333"
                     /isolate="AMR_MDS_5629"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1965333"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(197..3337)
                     /locus_tag="PLP58_19285"
     CDS             complement(197..3337)
                     /locus_tag="PLP58_19285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009958062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PSD1 and planctomycete cytochrome C
                     domain-containing protein"
                     /protein_id="HRJ10733.1"
                     /translation="MIFTRPHFTLPLLPALAAALSAHAATEADRARMQFFENEVRPLL
                     VQRCFECHAEKKQKGGLRVDNIGYLTAGGDSGPALVPGEPEKSLIIEAVRYHNAELEM
                     PPKEKLADKEIAALEQWVKIGAPWPESEARQVSMDEHGFTEEDRKFWSFQPLTNPKPP
                     VVKGGWVRNDIDRFVAAKHAELGLKPAPEADRRELVRRVYFTLHGLPPTPEQTRAFVS
                     STDPLAYEKLVDELLASPRYGERWAQHWLDLVRYAESDGYNQDALRPHAWPYRDYVVR
                     SFNADKPYDQFVREQLAGDELAPEDPEVLVATSYLRNPIYEYNQRDARGQYEVVLTDM
                     TDNAGEVFMGLSMGCARCHDHKFDPILQKDYYRLRAFFTPVRWRDDLTLATREEKARF
                     AMENAKWEEATKDIRAEMDAITEPMIQRAIENAYKRFQADIRAMVDKKPEEREPQDWQ
                     FSYFCERQMAYERERFDPMKSIKDEKVKARYLELVEELKKHDAIKPKPLLPAFIATDA
                     MPKGPPNVLKTRKGEEDIPPGFLTMLQPGLPEIKPLPNSTGRRSALAAWLTRKDNPFT
                     TRVITNRVWHYLFGRGIVETPNDFGKLGEQPTHPELLDYLTQKMLADGWKLKALQREI
                     LLSATFRQTAHIPVPEVAAKVDPANKYLWRFTPRRLDAEQVRDAMLAASGELDLSAGG
                     PSTDGNGLRRSIYTTKKRNSQNELLRALDAPAGFASTSERQSTTTPTQALLLVNGDWA
                     LSRASKLAERVYSIDEAWQYALGRAPTAKEVTLAESFIEKRQKEKAAPAAAPAVPMES
                     AAEFKENTPRERLIAQEAERTGDEFTVEAIVKLDSIDTAASVRTIASHWTGGKDNVES
                     FGWSIGVTGEKSRFKPRNLIIQLVGEDENRNIAYEPVASDLRVELGVTYHIAVKVSCA
                     AQEIVFRVQQVDKPNAPVLTSVVPHHVRVGLDRGVANLVIGGVHKRAPSHHWDGRIEA
                     ARITCGLLPDEALSAKPETWKAPALVNWNAKAGISERLAWSSAESPAVPLDPRRQAIV
                     DLCHVLLNANEFFYLH"
     gene            3593..5047
                     /locus_tag="PLP58_19290"
     CDS             3593..5047
                     /locus_tag="PLP58_19290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009962162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1501 domain-containing protein"
                     /protein_id="HRJ10734.1"
                     /translation="MNTPQDWHTFETRRRFFARGRNVVGYAALTSLLGEALTGRANAG
                     PGQVTTHFPAKAKRVIYLHMVGGPSQMDLFDYKPEMAKWYDKDLPESIRQGQRLTTMT
                     SGQARFPIAPSKFSFERRGQCGMWMNADLLPHLGTVADDICWMRSLHTQAINHEPAIC
                     AMQTGNQVTGRPCLGSWASYGLGSVNQNLPGFVVLIATPTNREQEQAVSPRLWSSGYL
                     PGQHAGVSFRSKGDPILFINNPPGVDDRVRRRMLDGVQALNEIAYQEVGDPETHTRIQ
                     QYEMAFRMQASVPELTDLSKEPGHTFKLYGEEAKKPGSFANSVLMARRLAERGVRFTQ
                     IYHNNWDHHSNVAGRMPSQCKDVDQPCAALIQDLKQRGMFEDTLIIWGGEFGRTIYSQ
                     GGLSKENYGRDHHPRCFTMWMAGGGAKGGAIHGETDDFSYNIVKDPLHISDFHATVLH
                     LLGYNHERFTFKYQGLDQRLTGVEPAKVVKALVA"
     gene            complement(5090..5524)
                     /locus_tag="PLP58_19295"
     CDS             complement(5090..5524)
                     /locus_tag="PLP58_19295"
                     /inference="COORDINATES: protein motif:HMM:NF012257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030551 - cyclic nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HRJ10735.1"
                     /translation="MPSLTPLLANFSHDELALLSSFGDSRSYAEDEILIRQGEENDHL
                     FLVLKGRLDVLQDVDGFNKKVATLEPGDSLGEVSVFDPGPASATVRSCGESEVWLITS
                     EGLDRLHSASPKVAYRLLSRITTCLSKRLRMMNDKVVDLVNR"
     gene            complement(5631..>6962)
                     /locus_tag="PLP58_19300"
     CDS             complement(5631..>6962)
                     /locus_tag="PLP58_19300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ10736.1"
                     /translation="LGGEIFAAIEDSDALTPDQKADLCCKMGARIVAQECAEAQGQPH
                     TLLRGNNAGTNFMSAFMNEHGADYFNAVKNAGLQAARQAQAAGLDPAQKQYAAAQAMM
                     TAMTTQAQLLEGPAANFLQATSQAARNAMPPDQAQAAVDKVMANTVALRGVCPAIIAE
                     SGVARRNAQIPPQEQAAHAVLFEAARGVQQFANVGGGGNVMGNKAECFDLIVEDEELG
                     EEFNNTLDLMSQGHQFPPPPVQQQAAPVVEQQVAPVVEQQANPDANALNEGAEKKSRL
                     ASVRDALRGAAGKIQDAAHDLGAGIKDKIEQAKLDRLDKQVQKRQDHGAYLQEQKDFA
                     DHVSRMSPEGRERLLADLQDKIDPDKLLKGDRQERAAQMQLMDQIEACKMSPEKLDQR
                     SAKLQEKLDKNEEKLGRQEQKRDAQKAKVDLRAARHANAQQQPQQAHAHGH"
BASE COUNT         1119 a         2362 c         2162 g         1319 t
ORIGIN      
        1 cagcaagcgc caccgcgcca aagccgccgc cggcgcggct gaggaactca cggcgtgagg
       61 tggggatgtc gtagttgtgg cagggcatgg tgggtaaagg tgcgggtttt tgaaccacta
      121 atggacgcta atgggcacta atttcagaaa tcgtaatctg tgtctgaatc ctctccaaag
      181 tcgcaaaacg cctgtctcaa tgcagataga aaaactcgtt cgcgttcagg agcacatggc
      241 aaagatccac gatggcctgg cggcgcgggt cgagggggac ggcggggctt tcggcgctgg
      301 accaggcgag gcgttcgctg ataccggctt tggcgttcca gttgacgagc gcgggcgctt
      361 tccaggtctc gggcttggcg ctgagagcct catccggaag caggccgcag gtaatgcgcg
      421 cggcctcgat gcggccatcc caatggtggc tcggcgcgcg cttgtgaacg ccgccgatga
      481 ccagattcgc cacgccacga tccagcccca cgcgcacatg atgcggcacc acggaggtga
      541 gcacgggcgc gtttggcttg tccacctgct gcacgcggaa aacgatctcc tgcgccgcgc
      601 acgaaacctt cacggcgatg tgatacgtca cacccagttc aacgcggagg tctgatgcca
      661 ccggttcgta ggcgatgttg cggttctcat cctcgcccac gagctggatg atcaggttgc
      721 gcggcttgaa gcgcgacttc tcacctgtga cgccgatgct ccagccaaag ctttccacat
      781 tgtccttccc gccagtccag tgggaggcga tggtgcgcac gctggcggcg gtgtctatgc
      841 tgtcgagctt cacgatggcc tccacggtga actcatcccc cgtgcgctcc gcctcctgcg
      901 cgatgagcct ctcacgcggt gtgttttctt tgaactcggc agcgctctcc atcggcaccg
      961 ccggggcggc ggcaggcgcg gctttttcct tctgccgctt ctcgatgaag gactccgcca
     1021 gcgtcacctc tttggccgtg ggcgcgcggc ccagcgcata ctgccaggcc tcatcaatcg
     1081 aatagacacg ctccgccagc ttgctcgcgc gggagagcgc ccagtcgcca ttcaccagca
     1141 gcagcgcttg tgtgggcgtg gtggtgctct ggcgctcgga ggtgctggca aagcccgccg
     1201 gggcatccag cgcgcgcagc agttcattct gcgagttgcg cttcttcgtg gtgtaaatgg
     1261 agcggcgcag gccgttgcca tccgtggagg gtccgcccgc cgaaaggtcc agctcgccgc
     1321 tggccgccag catggcatca cgcacctgct cggcatccag gcggcgcggg gtgaagcgcc
     1381 acagatactt gttcgccggg tccaccttgg ccgccacttc tggcacgggt atgtgcgccg
     1441 tctggcggaa ggtggccgag agcaatatct cccgctgcag cgccttcagc ttccagccat
     1501 ccgccagcat cttttgcgtc aggtagtcca gcagctcggg gtgcgtgggc tgctcgccga
     1561 gtttgccgaa gtcattcggc gtctccacga tgccgcggcc aaacaaataa tgccacacgc
     1621 gattggtgat cacgcgcgtt gtgaagggat tgtccttccg tgtcagccat gccgccaggg
     1681 cgctgcgccg accggtggag ttcggcagtg gtttgatctc cggcagaccg ggttgcagca
     1741 tcgtcaggaa gcccggaggg atgtcttcct cgcccttgcg ggttttcaaa acattcggcg
     1801 gccccttcgg catcgcgtcc gtggcgatga aggccggcaa aagcggcttc ggcttgatgg
     1861 cgtcatgctt cttcagctcc tccaccagct ccaggtagcg cgccttcacc ttctcatcct
     1921 tgatggactt catcgggtca aagcgctccc gctcataggc catctggcgc tcgcagaagt
     1981 agctgaactg ccagtcctgc ggctcgcgct cctcgggctt cttgtccacc atggcgcgga
     2041 tgtccgcctg gaaacgcttg taggcgttct cgatcgcgcg ctggatcatc ggctcggtga
     2101 tggcgtccat ctccgcgcgg atgtccttcg tggcctcctc ccacttcgcg ttctccatgg
     2161 caaagcgcgc cttttcctcc cgcgtcgcca gagtcaggtc atcccgccag cgcaccgggg
     2221 tgaagaaggc gcgcaggcgg tagtagtcct tttggaggat ggggtcgaac ttgtggtcat
     2281 ggcagcgcgc gcagcccatg ctcaggccca tgaagacctc ccccgcattg tctgtcatgt
     2341 ctgtgagcac cacctcatac tgcccgcgcg cgtcgcgctg attgtattca tagatcgggt
     2401 tgcgcaggta agacgtggcc accagcacct ccgggtcctc cggtgccagc tcatcccccg
     2461 cgagttgctc gcgcacgaac tggtcgtacg gcttgtccgc attgaagctc ctcaccacat
     2521 agtcgcggta aggccaggca tgaggccgca gcgcgtcttg attgtaacca tcactctccg
     2581 cgtagcgcac caaatccagc caatgctgcg cccagcgctc gccatagcgc ggactggcca
     2641 gcagctcatc caccagcttc tcataggcca gcgggtccgt gctgctcaca aaagcccgcg
     2701 tctgctcagg cgtcggcggc aggccgtgca gggtgaaata cacacgccgc accagctccc
     2761 ggcggtccgc ctcgggcgcg ggcttcaggc ccagctccgc gtgcttcgcc gccacgaagc
     2821 ggtcaatgtc attgcgcacc cagccgccct tcaccaccgg cggcttcgga ttcgtcaacg
     2881 gctggaagct ccaaaacttc cggtcctcct ccgtgaagcc gtgctcgtcc atggacacct
     2941 gccgcgcctc gctctccggc cagggcgcgc caatcttcac ccactgctcc agcgccgcga
     3001 tctccttgtc cgccagcttc tccttcggcg gcatctccag ctccgcgttg tgatagcgca
     3061 ccgcctcgat gatcagcgac ttttcaggct cccccggcac cagcgccggc cccgagtccc
     3121 cgcccgccgt caggtagccg atgttgtcca cgcgcaggcc gcccttttgc ttcttttccg
     3181 catggcattc aaagcagcgc tgcaccagca gcggccgcac ctcgttttca aagaactgca
     3241 tccgcgcccg gtccgcctcc gtggcggcat gggcggaaag cgccgccgcc agagcgggca
     3301 gcagcgggag ggtgaaatgc gggcgagtga aaatcatgag gcgggaaagg aagccggaac
     3361 cttgaagcac catcaggcgc caaagccccg gctttggaca aaaaaatcac agccaggcgc
     3421 aagcgacggg tgatgtgacg ctgggcaaaa cctacgcgcg cgggccgggg aatcttcgcg
     3481 cgggcatccg ccggcaagat tgatgcatgc cacatcgccc atgccgcgcc ttgttgcacg
     3541 atccccgcac tgcggatcgt attgatctgg caaatccccc catctctccg tcatgaacac
     3601 tccgcaggac tggcacactt ttgaaactcg ccggcgcttt ttcgcgcgcg ggaggaatgt
     3661 cgtcggctat gccgccctca cctccctgct gggcgaggcg ctcaccggcc gtgcgaatgc
     3721 cggccccggc caagtgacca cccatttccc cgccaaggcc aagcgcgtca tctacctgca
     3781 catggtcggc ggcccctcgc agatggacct cttcgattac aagccggaga tggccaaatg
     3841 gtatgacaag gacctgcccg aaagcatccg ccagggccag cgcctcacca ccatgaccag
     3901 cggccaggcg cgcttcccca tcgcgccgtc gaagttcagc ttcgagcggc gcgggcagtg
     3961 cggcatgtgg atgaatgcgg acctgctgcc gcatctcggc accgtcgccg atgacatctg
     4021 ctggatgcgc tctctccaca cccaggccat caaccacgag cccgccatct gcgccatgca
     4081 gaccggcaac caggtcaccg gccgcccctg cctcggctcc tgggcctcct acggcctcgg
     4141 ctccgtgaat caaaacctgc ccggctttgt cgtgctcatc gccaccccca ccaaccgcga
     4201 gcaggagcag gccgtctccc cccgcctctg gtccagcggc tacctgcccg gccagcacgc
     4261 cggcgtgtcc ttccgcagca agggcgaccc gatcctcttc atcaacaacc cccccggcgt
     4321 ggacgaccgc gtgcgccgcc gcatgctcga cggcgtccag gccctcaacg agatcgccta
     4381 ccaggaagtc ggcgaccccg agacccacac ccgcatccag caatacgaaa tggccttccg
     4441 catgcaggcc agcgtgccgg agctgacgga cctctccaaa gagcccgggc acaccttcaa
     4501 gctctatggt gaggaggcca aaaagcccgg ctccttcgcc aacagcgtcc tcatggcccg
     4561 ccgcctcgcc gagcgcggcg tgcgcttcac ccagatctac cacaacaact gggaccacca
     4621 ctccaacgtc gccggccgca tgcccagcca gtgcaaggat gtggaccagc cctgcgccgc
     4681 cctcatccag gacctcaagc agcgcggcat gttcgaggac accctcatca tctggggcgg
     4741 cgaattcgga cgcaccatct acagccaggg cggcctctcc aaggaaaact acggccgcga
     4801 ccaccacccc cgctgcttca ccatgtggat ggccggcggc ggagccaaag gcggcgccat
     4861 ccacggcgag accgacgact tcagctacaa catcgtcaag gaccccctcc acatcagcga
     4921 cttccacgcc accgtcctgc acctgctcgg ctacaaccac gagcgcttca ccttcaagta
     4981 ccagggcctg gaccagcgcc tcaccggcgt cgagcccgcg aaggtggtga aggcgctggt
     5041 ggcgtgacgc atccgccgcg cgtcacgcgg ggctttcaca cgcgcgccct caccggttca
     5101 ccaggtccac gaccttgtca ttcatcatgc gcaggcgctt ggagaggcag gtggtgatgc
     5161 ggctgagcag gcggtaggcc accttggggc tggcggagtg caggcggtcg agcccctcgg
     5221 aggtgatgag ccagacctcg gactcgccgc aggagcgcac ggtggcgctg gcggggccgg
     5281 gatcgaagac gctgacctcg ccgagggagt cgcccggctc cagggtggcg acttttttgt
     5341 taaaaccgtc cacgtcctgg agcacgtcga gcctgccctt gaggacgagg aagagatggt
     5401 cgttctcctc gccctggcgg atgagaatct catcctcggc gtaggagcgg gagtcgccga
     5461 agctggagag cagggcgagt tcatcatggg agaagttggc gaggaggggg gtgagggagg
     5521 gcatggctgg aggcagagtg cctggtgaat gggtggagtc cagaggttat gagcgcgccc
     5581 gccggatcat gggctggcgg tgcggccagt ggggcgtgct tgttgtcaat tcagtggcca
     5641 tgcgcgtgcg cctgctgcgg ctgctgctgg gcgttggcgt ggcgcgcggc gcgcaggtcc
     5701 accttggctt tttgagcatc ccgtttttgc tcctgcctgc cgagtttttc ctcgttcttg
     5761 tcgagcttct cctgaagttt ggcggagcgc tggtcgagct tctcggggga cattttgcag
     5821 gcttcgatct gatccatgag ctgcatttgg gcggcccgct cctggcggtc gcctttgagc
     5881 agcttgtccg ggtctatctt gtcctggaga tccgccagca gcctctcccg gccttcgggg
     5941 gacatccggc tgacatggtc cgcgaagtcc ttctgctcct gaagatacgc gccgtggtcc
     6001 tggcgcttct ggacctgctt gtcgaggcgg tcgagcttgg cctgctcgat cttgtccttg
     6061 atgccggctc ccagatcgtg ggcggcgtcc tggatttttc ccgccgcgcc gcgcagggcg
     6121 tcgcgcacgc tggcgagtct ggattttttc tccgctccct cgttcaaggc attggcgtcc
     6181 gggttcgcct gctgctcgac gacgggtgcg acctgctgct cgacgacggg tgcggcctgc
     6241 tgctggacgg gcggcggcgg gaactggtgt ccctgactca ttaaatccag cgtgttgtta
     6301 aactcctctc ccagctcctc atcctccaca atcaggtcaa agcattcggc tttgttgccc
     6361 atgacattgc cgccaccacc gacgttggcg aactgctgga ccccccgggc ggcctcgaag
     6421 agcacggcgt gggctgcctg ttcctggggg gggatctgcg cgttcctgcg cgcgacgccg
     6481 gattccgcga tgatggcggg gcagactccg cgcagtgcga ccgtgttggc catgactttg
     6541 tccaccgcgg cttgagcctg gtccggcggc atggcgtttc tcgcggcctg gctggtggcc
     6601 tggaggaagt tggcggccgg tccctccagc agttgcgcct gggtggtcat cgccgtcatc
     6661 atcgcctgcg ccgccgcgta ttgtttctgg gccgggtcca ggccggctgc ctgcgcctgc
     6721 cgcgccgcct ggagtccggc gtttttgacc gcgttaaagt aatccgcccc atgctcgttc
     6781 atgaacgcgc tcatgaagtt ggtgccggcg ttgttcccgc gcagcagggt gtgcggctgc
     6841 ccctgcgctt cagcgcactc ttgcgcgacg atcctggccc ccatcttgca gcagagatcg
     6901 gccttctggt ccggggtgag cgcgtcgctg tcctcgatgg ccgcgaagat ttccccgccc
     6961 ag
//
LOCUS       DAOMYJ010000256         6911 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Prosthecobacter sp. isolate AMR_MDS_5629
            NODE_3702_length_6911_cov_21.615373, whole genome shotgun sequence.
ACCESSION   DAOMYJ010000256 DAOMYJ010000000
VERSION     DAOMYJ010000256.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867850
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prosthecobacter sp. (wastewater metagenome)
  ORGANISM  Prosthecobacter sp.
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Verrucomicrobiaceae; Prosthecobacter.
REFERENCE   1  (bases 1 to 6911)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6911)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.30
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:34:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,742
            CDSs (total)                      :: 4,684
            Genes (coding)                    :: 4,676
            CDSs (with protein)               :: 4,676
            Genes (RNA)                       :: 58
            tRNAs                             :: 55
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6911
                     /organism="Prosthecobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3702_length_6911_cov_21.615373"
                     /isolate="AMR_MDS_5629"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1965333"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..443
                     /locus_tag="PLP58_19305"
     CDS             <1..443
                     /locus_tag="PLP58_19305"
                     /inference="COORDINATES: protein motif:HMM:NF023197.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF3313 family protein"
                     /protein_id="HRJ10737.1"
                     /translation="LREEFARAFRESPGPRQEVVAAPAADSLLVELALVELDPNPVSG
                     GVTRRFINLLTFPGAESVVGDPLKGRTAIEGRLYDPVQKQVLLEFTDVEQNRSALILS
                     LHDYNHYSHARKVAREWARQFEALVSMPPGARVKDSPAFMIWLW"
     gene            complement(453..665)
                     /locus_tag="PLP58_19310"
     CDS             complement(453..665)
                     /locus_tag="PLP58_19310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ10738.1"
                     /translation="MRPILYWLSSLFVAVIAAKAAGRADSETISLDAAMPLLAVADPV
                     RSVLLGAGVLALAFTYRQAWAAFRGR"
     gene            855..2810
                     /locus_tag="PLP58_19315"
     CDS             855..2810
                     /locus_tag="PLP58_19315"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HRJ10739.1"
                     /translation="MNASQTSNHRPDIDGLRGLAVLLVVLFHARLSFRGGFIGVDVFF
                     VISGYLITGIMVRDLERGGFSLLEFWERRARRILPALTVLVLAVLGLGFWLMLPGDLD
                     RLGSSAAAQALLAANVYFWREGGGYFAGPAEQMPLLHLWSLAVEEQFYLLMPFLCMAL
                     AAWQRRGGGRDLRGLCLRVFGALLLLSLAVSAVMVEADPHWAFYWLPARAWELLLGSV
                     LACLPAAGRFQQALKRRQGLVSGASLCVIVGTALAYDNDTPFPGLAALPPCLAAGLLT
                     GAHATNGAGWARRVLESAPLRHLGLVSYSLYLWHWPLLAFVSYLSPVVLDADDRVCRL
                     AVVLAAWGLAVLSWRWVETPVRRRTLLGRRRPLLQTAALALAVTFAVGWSLRGGLPGR
                     IKPEVLALASAESAAKEWPDDAGAALENRLPKFGAKAAEAPARVLIWGDSHAKSIVPA
                     LAAWCVEERAAGRVAAYSATAPVIGFHRKSRGGLNTRSHAWSAAVLEIIRREKIPDTV
                     LTALWSKCPGDDPAAFGAALLDTVRLLREAGTRVWIMLDAAGHDFIVSKALAFHTMHP
                     AVFRDPRRLGRTLQDYRDDNAVIESLRPALEKAGARVLDPAPLLFGPDGRALIEADGK
                     ALYQDDNHLTHDGALHLKSLFEPVFKN"
     gene            2874..3932
                     /locus_tag="PLP58_19320"
     CDS             2874..3932
                     /locus_tag="PLP58_19320"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HRJ10740.1"
                     /translation="MRVSLLHYTLPPAIGGVERVVGTQAEALRSLGHEVSLWPAQQKA
                     EFAAALARGAVDAVIVHNVFTMPFDLEWTRELTRLALARKDVLWINWVHDVAAVNPHY
                     AGIPWKEPAPRALHVAVSEARRRDYERAAGLRDIRVIPNGLDMAQVLGLTRRVKELGL
                     ESCGLVLFHPARLVRRKNIELGIRVTSALRKAGVDALYLVSGAPDPHQADGRAYHAEL
                     CRLAGELGLDDGVRFLGEDGPLCEADVRALYAAADLLFFPSTGEGFGLPLLEAAAHRL
                     PVWCSDLPVHREVLGDGAHFFPANSPPETISAQIMRWWRADVPDACRKSLWRRHDMLK
                     ICLEHLEPLLLAHTHDHD"
     gene            3925..6108
                     /gene="glgB"
                     /locus_tag="PLP58_19325"
     CDS             3925..6108
                     /gene="glgB"
                     /locus_tag="PLP58_19325"
                     /EC_number="2.4.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009962912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003844 - 1,4-alpha-glucan branching
                     enzyme activity [Evidence IEA];
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0043169 - cation binding [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-alpha-glucan branching protein GlgB"
                     /protein_id="HRJ10741.1"
                     /translation="MTEDTGFSADALALLEARHSDPFGFLGMHECAGGVVVRAFHPRA
                     QSARVTARDGSGSWEMSREHHHGLFSVTVTGHGCFPYDIEFTSYEGRVTRGADPYSFG
                     SLLGEQDIYFFREGTHQRLWDCLGARLRVVDGIPGAQFAVWAPNARRVSVVGDFNHWD
                     GRVHPMRLRVEGGVWEIFLPGITELTHYKFEILTAEGHLLLKTDPMGFFGQHGTQTAS
                     LVFDLERYRWSDQEWMNRRATHDLYHTPMSIYEVHLGSWKRIPEQGNRPKSYLELADD
                     LIPYVKSLNFTHIELMPVAEHPFDGSWGYQVTGYYAPTSRFGNPDEFRQFVDRCHQAG
                     IGVILDWVPGHFPKDAHGLAKFDGSALYEHADPRQGEHQDWGTLIFNYGRAEVKNFLV
                     ANALFWLEQYHIDGLRVDAVASMLYLDYSRKPWAWVPNKYGGRENLEAIDFMRDLNRV
                     CYEKHPGTTIIAEESTAWPGVSKPTDAGGLGFGFKWNMGWMNDTLHYMQEDPVHRKYH
                     HGEATFSMLYAYDENFILVISHDEVVHGKGSLINKLPGDRWQKFANLRMFFAWMWTHP
                     GKKLIFMGSEFGQWSEWNHARSLDWHLFLGEEHAAAQKLVQDLNWLYQNTPALHQLDH
                     EPGGFDWLDASDADQSIFAYTRADREGGIVYVIVNATPVPRKGYRVGVARPGSYRELL
                     NTDAPKYAGSGMVNPLPLQSHDIPWHGLAQSIIVDIPPLGASILA"
     gene            6181..>6911
                     /locus_tag="PLP58_19330"
     CDS             6181..>6911
                     /locus_tag="PLP58_19330"
                     /inference="COORDINATES: protein motif:HMM:NF037667.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ10742.1"
                     /translation="MRKIIAFILAGVASAGAEALHECGHCAKHLLPSVTKLTPGRKYA
                     RDRRVDIVHLKLEVVPDFSKRTVAGLMTMDFSPVGLPLEKLELDAVDLAVTEVSANGA
                     ALADWHVGEEKLVLHFDRPAAPGAKLQVRARWTAAPERGLYFRTPEMGYKPGDTQLWT
                     QGEADLHRFWFPCYDYPNERFSSEIVCDAPAGMEVISNGVLLGKAPAGDLVRWHWRQD
                     KPHVNYLVALAAGYFHKLEDKAGELP"
BASE COUNT         1117 a         2222 c         2332 g         1240 t
ORIGIN      
        1 acctgcgcga ggagttcgcg cgggcgttcc gcgagtcgcc cggcccgcgc caggaagtgg
       61 tggcagcgcc cgcggcggac agcctgctgg tggagctggc gctggtggaa ctggacccga
      121 atcctgtctc cgggggagtc acgcgccggt tcatcaacct gctgaccttt cccggcgcgg
      181 agtccgtggt gggggacccg ctcaaagggc gcaccgccat cgaggggcgg ctctatgatc
      241 cggtgcagaa gcaggttttg ctggagttca ccgatgtgga gcagaaccgc tcggcgctca
      301 tcctctccct gcacgactac aaccactaca gccatgcgcg caaggtggcg cgcgagtggg
      361 cgcggcagtt cgaggcgctg gtgagtatgc cgccgggtgc cagggtgaag gacagccccg
      421 ccttcatgat ctggctctgg tgacgcgcgc gctcaacgcc cgcggaatgc cgcccatgcc
      481 tggcggtagg tgaaggcaag ggccagaact ccggctccaa gcagcacgga gcgcaccggg
      541 tctgcgaccg ccagcagggg catggccgcg tccagggaaa tggtctctga atccgcgcgg
      601 cccgcggctt tcgcggcgat cacagccaca aagagggaac tcagccaata aaggatggga
      661 cgcatttttt gaatttattg gcctggtttt atctcgtccg gcaggcttgg ggcaagtaaa
      721 tacattgtgc ttcccaaaaa agtgcctcca gtcagcgccg ccggctgccg caggctcgcc
      781 cgatggaggg acgccggccc tcccgccgcg ttgagcttgc gccgcgaggc ggcaggcgta
      841 tcggggcggc ttcgatgaat gcatcacaga cctccaacca caggccggac atagacgggc
      901 tgcgcgggct ggccgtgctc ctggtggtgc tgtttcacgc gcgcctttcc ttccggggcg
      961 ggttcatcgg agtggatgtg tttttcgtca tctccggcta cctgatcacg ggcatcatgg
     1021 tcagggacct ggaacgggga ggcttcagcc tgctggagtt ttgggagcgg cgcgcgcggc
     1081 gcatcctgcc cgcgctcacg gtgctggtgc tggcggtgct gggtctcggc ttctggctga
     1141 tgctgcccgg tgatttggac cggttgggaa gctcggcggc ggcgcaggcg ctgctggcgg
     1201 ccaatgttta tttctggagg gaaggcggag gctattttgc cgggcctgcg gagcagatgc
     1261 cgctgctgca tctgtggtcg ctggcggtgg aggagcagtt ttatctgttg atgcccttcc
     1321 tgtgcatggc gctggccgca tggcagcgga gaggcggggg ccgggatttg cgcggcctgt
     1381 gcctgcgcgt cttcggggcg cttttgctgc tgagtttggc ggtttcagcc gtgatggtcg
     1441 aggcggaccc gcattgggcg ttttactggc tgcctgcgcg cgcatgggag ctgctgctgg
     1501 gctcggtgct ggcctgcctg ccagcggcgg gccggtttca gcaggcgttg aaaagacggc
     1561 agggactcgt gtcgggcgct tccctttgcg tcatcgtcgg gacggctctc gcctatgaca
     1621 atgacacgcc ttttcccgga ctcgcggctc tgcctccgtg cctggccgcc ggcctcttga
     1681 caggggcgca tgcaacgaac ggcgcgggct gggcgcgccg tgtgctggag tcggctccgc
     1741 tgcgccatct cggcctggtc tcgtactcgc tttatctctg gcactggccg ctgctggctt
     1801 tcgtgagcta tctcagcccg gtggtgctgg atgcggacga ccgggtttgc aggctggcgg
     1861 tcgtgcttgc cgcctgggga ctggcggtgc tctcctggcg ctgggtggag acgccggtgc
     1921 ggagacggac gctgctcggc cggagaaggc ctcttttgca aaccgccgcc cttgcgttgg
     1981 cggtgacttt tgccgtcggc tggagcctgc gcggaggctt gcccgggagg atcaaaccgg
     2041 aggtgctggc gctggcctcg gcggagtccg ccgcgaagga gtggccggat gatgccgggg
     2101 cggcgctgga aaacaggctg ccgaagttcg gagcaaaggc agccgaagct cccgcgcggg
     2161 tgctgatctg gggggacagc cacgccaaaa gcatcgtgcc cgcgctggcg gcgtggtgcg
     2221 tggaggagcg ggctgccgga cgggtggccg cgtactccgc cacggcgcca gtcatcggat
     2281 tccaccgcaa aagccggggc gggctgaaca cccgcagcca cgcctggagc gcggcggtgc
     2341 tggagatcat acgccgggaa aaaatcccgg acacggtgct gacggcgctt tggagcaaat
     2401 gcccgggcga cgatccggcg gcattcggcg cggcgctgct ggacacggtg cggctgctgc
     2461 gcgaggccgg cacccgcgtc tggatcatgc tcgatgcggc gggtcatgac ttcatcgtct
     2521 cgaaagccct ggcctttcac accatgcacc ccgccgtttt ccgggatccg cgccgcctgg
     2581 ggcggactct gcaggattac cgcgatgaca acgcggtgat cgaatccctc cgcccggcgc
     2641 tggagaaagc gggcgcgcgc gttctcgatc ccgcgcccct cctgttcggc ccggacgggc
     2701 gcgcgctgat cgaagcggac ggaaaagccc tctaccagga tgacaaccac ctgacgcatg
     2761 acggcgcgct gcatttgaaa agcctcttcg agccggtctt caaaaactaa acccgccggg
     2821 cgggggcgcg cactttcgga tttcgatttt cccattccgg gcttccatga agcatgcgcg
     2881 tctccctgct ccattacacc ctgcctccgg ccatcggcgg agtggagcgg gtggtgggga
     2941 cgcaggcgga ggccttgcgc agcctggggc acgaggtgag cctgtggccg gctcaacaaa
     3001 aggcggagtt cgcggcggcg ctcgcgcgcg gggcggtgga cgcggtcatc gtgcataacg
     3061 tgttcacgat gccgttcgac ctggagtgga cgcgggagct gacgcgcctg gcgctggcgc
     3121 gaaaggacgt gctgtggatc aactgggtgc atgacgtggc ggcggtgaat ccgcattacg
     3181 cgggcatccc gtggaaggaa ccggcgccgc gcgcgctgca tgtggcggtt tcggaggccc
     3241 ggcggcggga ctatgaaagg gccgccggtt tgcgggacat ccgcgtcatc ccgaacggcc
     3301 tcgacatggc gcaggtgctg ggactgacgc ggcgcgtgaa ggagctggga ctggaaagct
     3361 gcggtcttgt gctgttccat cccgcgcggc tggtccggcg gaaaaacatc gagctgggaa
     3421 tccgtgtcac gtcagcgctg cgcaaggcgg gtgtggacgc gctctacctt gtcagcggcg
     3481 cgccggaccc gcaccaggcg gacgggaggg cataccacgc ggagctttgc cgtctggccg
     3541 gcgagcttgg cctggatgac ggggtgcgct ttctcggcga ggacggaccg ctctgcgagg
     3601 cggatgtgcg cgccctgtat gcggcggcgg accttttgtt tttccccagc acgggggagg
     3661 gtttcggact gccgctgctg gaggcggcgg cgcaccggct gccggtatgg tgctcggacc
     3721 tgcctgtgca tcgcgaggtg ctgggggacg gagcacattt ttttccggca aattcgccgc
     3781 ctgagaccat ctcggcacag atcatgcgct ggtggcgcgc cgatgttccg gacgcatgcc
     3841 gcaagtcgct ctggaggcgg catgacatgc tcaaaatctg cttggaacat cttgagcctc
     3901 tgctacttgc acatacccat gatcatgact gaagacaccg gattttccgc cgacgcgctc
     3961 gccctcctgg aagcccggca cagtgatccg tttggtttcc tcggcatgca tgaatgcgcc
     4021 ggcggagtgg tcgtgcgcgc cttccacccg cgcgcccaaa gcgcccgcgt cacagcgcgc
     4081 gacggctccg gctcctggga gatgagccgc gagcatcatc acggcttgtt ttccgtgact
     4141 gtcaccgggc atggctgctt cccctacgac atcgagttca ccagctacga gggccgggtc
     4201 acgcgtggcg cggaccccta ttccttcggt tcgctgctgg gtgagcagga catttatttt
     4261 ttccgcgaag gcacccacca gcggctctgg gactgcctcg gcgcgcgcct gcgcgtggtg
     4321 gacggcatcc ccggcgcgca gttcgccgtc tgggcgccca atgcgcgccg cgtctccgtg
     4381 gtgggcgact tcaatcactg ggacggccgc gtgcatccca tgcgcctgcg cgtcgagggc
     4441 ggcgtctggg agatctttct gcccggcatc accgagctga cgcattacaa attcgaaatc
     4501 ctcaccgccg aggggcacct gcttttgaag accgatccca tggggttctt cggccagcac
     4561 ggcacacaga ccgcaagtct cgttttcgac ctcgagcgct accgctggag cgaccaggag
     4621 tggatgaacc gccgcgccac ccacgatctc taccacacgc ccatgagcat ctacgaggtg
     4681 catctcggct cgtggaagcg catccccgag cagggaaacc ggcccaagtc ctacctcgaa
     4741 ctcgccgacg acctcatccc gtatgtgaag tcgctgaatt tcacccacat cgagctgatg
     4801 cccgtggcgg agcacccctt cgacggctcc tggggctacc aggtcaccgg ttactacgct
     4861 cccaccagcc gctttgggaa tccggatgag ttccgccagt tcgtggaccg ctgccaccag
     4921 gcgggcatcg gcgtcatcct cgactgggtg cccgggcatt ttccaaagga cgcccacggc
     4981 ctcgccaaat tcgacggcag cgccctctac gagcatgccg atccgcgcca gggcgagcac
     5041 caggactggg gaacgctcat cttcaactac ggccgcgccg aggtgaagaa ctttctcgtc
     5101 gcgaacgccc tcttctggct ggagcagtac cacattgacg gcctgcgcgt ggacgccgtg
     5161 gcctccatgc tctatctcga ctactcccgc aagccctggg cctgggtgcc caacaaatac
     5221 ggcgggcgcg agaacctcga agccattgac ttcatgcgcg acctcaaccg cgtctgctac
     5281 gagaagcacc ccggcaccac catcatcgcc gaggaaagca ccgcctggcc cggcgtctcg
     5341 aagcccaccg atgcgggcgg cctcggcttc ggtttcaaat ggaacatggg ctggatgaac
     5401 gacaccctcc actacatgca ggaggatccc gtccaccgga agtatcacca cggcgaggcc
     5461 accttctcca tgctctacgc ctacgacgag aacttcatcc tcgtcatcag ccacgacgag
     5521 gtcgtgcatg gcaagggcag cctcatcaac aaacttcccg gcgaccgctg gcaaaagttc
     5581 gccaatctgc gcatgttctt cgcctggatg tggacccacc caggcaagaa gctcatcttc
     5641 atgggcagcg agttcggcca gtggagcgaa tggaaccacg cccgcagcct cgactggcat
     5701 ctctttctcg gcgaggagca cgccgccgcg caaaagctcg tgcaggacct caactggctt
     5761 taccaaaaca cgcccgccct ccaccagctc gaccacgagc cgggcggatt cgactggctc
     5821 gacgccagcg acgccgacca gagcatcttt gcctacaccc gcgccgaccg cgagggcggc
     5881 atcgtttatg tcatcgtcaa tgccacccct gtcccgcgca aaggctaccg tgtcggagtc
     5941 gcccgccccg gctcctaccg ggagttgctg aacacggacg ctcccaaata cgccggcagc
     6001 ggcatggtca acccgctgcc cctgcaaagc cacgacatcc cctggcacgg cctggcccaa
     6061 agcatcatcg tggacatccc gccgctcgga gcgagcattc tggcctgagt ctgccagact
     6121 ttgccgcggg cgatttttcc cccttgccaa gccgccgctc cgcccgccat gctgcgggtc
     6181 atgagaaaaa tcatcgcttt cattctggcg ggagtcgcat cagcgggcgc ggaggcgctg
     6241 catgagtgcg ggcactgcgc caagcatctg ctgccctcgg tgacgaagct cacccccggc
     6301 cgcaaatacg ctcgcgaccg ccgggtggac atcgtgcact tgaagctgga ggtggtgccg
     6361 gacttctcca aaaggaccgt ggccggactg atgacgatgg atttctcacc ggtcgggctg
     6421 ccgctggaaa agctggagct ggacgccgtg gacctggcag tgaccgaggt ctccgccaat
     6481 ggcgcggcgc tcgcggactg gcatgtgggc gaggaaaagc tcgtgctgca ttttgacagg
     6541 cccgcggctc caggcgcgaa gctgcaagtg cgcgcgcgct ggacggcggc tccggagcgc
     6601 ggcttgtatt tccgcacgcc ggagatgggc tacaaaccgg gcgacacgca actctggacg
     6661 cagggggagg cggatctgca ccgcttctgg ttcccctgct acgattaccc gaacgagcgc
     6721 ttcagcagcg agattgtctg cgatgcgccc gccgggatgg aggtcatctc caatggcgtg
     6781 ctgctgggca aggcgcccgc cggtgatctt gtgcgctggc attggcggca ggacaagccg
     6841 catgtgaact acctcgtggc gctggcggcg ggctatttcc acaagctgga ggacaaggcc
     6901 ggcgagctgc c
//
LOCUS       DAOMYJ010000257         6907 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Prosthecobacter sp. isolate AMR_MDS_5629
            NODE_3705_length_6907_cov_21.186369, whole genome shotgun sequence.
ACCESSION   DAOMYJ010000257 DAOMYJ010000000
VERSION     DAOMYJ010000257.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867850
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prosthecobacter sp. (wastewater metagenome)
  ORGANISM  Prosthecobacter sp.
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Verrucomicrobiaceae; Prosthecobacter.
REFERENCE   1  (bases 1 to 6907)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6907)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.30
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:34:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,742
            CDSs (total)                      :: 4,684
            Genes (coding)                    :: 4,676
            CDSs (with protein)               :: 4,676
            Genes (RNA)                       :: 58
            tRNAs                             :: 55
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6907
                     /organism="Prosthecobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3705_length_6907_cov_21.186369"
                     /isolate="AMR_MDS_5629"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1965333"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1219
                     /locus_tag="PLP58_19335"
     CDS             <1..1219
                     /locus_tag="PLP58_19335"
                     /inference="COORDINATES: protein motif:HMM:NF024794.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="M60 family metallopeptidase"
                     /protein_id="HRJ10743.1"
                     /translation="SAPAAHPAHESFPGKAPAAAPRVSREVSVNPAIPGWTSTGLYAA
                     AGEPVRVQIPAERAGKGYALRIGCHSDTLYHLDSWLRAPEITKSVSLDMAETTVSSAF
                     GGLIYIVVPDKAADGTRFTCALHGGIPAPHFVLGLHSDAQWAGEISRHPAPWAEFECG
                     NIILSCPADTARAVRNPAQLMEFWKKVVEAQDEITNQAAERRRPERIVADVQISAGYM
                     HSGYPIMIPVSAAEEMVTFNRLKFPGWGFYHEIGHNHQRPHFTFEGTGEVTNNVISMW
                     CYEAVLGKDWLIGHPNITAEARAEHIQKIKRASDKWAVWKSDPFLALTTYIQLVQGFG
                     WDSWRKYLHSFADPAHGPMPAGDDERRDQFLVRYSKIVNRDLGPLFDFWGIPVSPAAK
                     ASVAGLEPWMPKM"
     gene            1355..2161
                     /locus_tag="PLP58_19340"
     CDS             1355..2161
                     /locus_tag="PLP58_19340"
                     /inference="COORDINATES: protein motif:HMM:NF024664.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4058 family protein"
                     /protein_id="HRJ10744.1"
                     /translation="MNKNPFPGMNPFMEALWPDIHTALIGYIRDSIAEQLPPDLNARS
                     EESVTLHDDLNTPPSKARADVAVTESWRQGIPPSWNPAQETDGPARTLSRPQIILVEE
                     EVERWIEITDRHGLLITVIEVLSPANKGAHREEYRSKRDAFLHAGVNVVEIDLLHQGR
                     HTVAVALESVRMSAPGQHYLIRVTRATRPSAREVYAAPLREPLPVISIPLRAKDKDAV
                     LALQPLVDRCYRMGAYWNANHDTLPEPAPAPDDEEWIRSRVKAAREQAAA"
     gene            complement(2177..3274)
                     /locus_tag="PLP58_19345"
     CDS             complement(2177..3274)
                     /locus_tag="PLP58_19345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ10745.1"
                     /translation="MNIRTSILAAALLFNAVSFPGQEAETPIDWDRARQLFQREQRGE
                     KLAAEDQKYLDRAKEARRKGAARPEAGAGQRKAPESLRPLCDMTAEDRYEGQDGGLYG
                     GGMNEPPEALRKTAAAALAQIQPLNREGKPADDGRVVLVSISMSNATQEFSVFKRIAD
                     ADPRKFAKLTIVDCAQGGQAMAEWAPPAGRPWEEAMRRISAAGVTAAQVQVAWIKLAN
                     KSPSGGMSEHLARLEADTTAVLRNARERFPNLRVAYLGSRIWAGNARGALNPEPYAYE
                     SAFAARNLIRRQMSGDDSLAAGRAPLLLWGPYLWAEGEKGRKLDGLKYAPDDFAADGV
                     HPSNSGREKVARLLLEFFATDPLAKSWFAGK"
     gene            3413..4606
                     /gene="thiH"
                     /locus_tag="PLP58_19350"
     CDS             3413..4606
                     /gene="thiH"
                     /locus_tag="PLP58_19350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009964277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-iminoacetate synthase ThiH"
                     /protein_id="HRJ10746.1"
                     /translation="MLPAQAFPPTTTLMPADFLTTLDALPDRKSPLMERFWSMIGPKA
                     PARIEAMAREARAVTRRNFGRTMRLFAPLYLSNECVNNCAYCGFSRDNPDILRVTLSV
                     DQVVREARHLAEQGFRNVLLVAGEHPRFVSEGYLEECIRAIRGFIPTVGIEVGPMEAP
                     EYARMVEAGAEGLVVYQETYDREAYAEMHTAGPKKDFAWRMACPERGHEGGFRRIGIG
                     ALFGLSDWRLEALRLAAHLEHLLRHCWRASFTVAFPRLRPAAGGFQPLTAFPDWALVQ
                     TLCAFRLTFPEAGIVLSTREPAALRDALAPLGVTSMSAGSHTEPGGYTGAGAEDLHKT
                     VKGRRVDLEDKPCSSRAEGQFHIADERSPAEVAAMLRARGLDPVWKDWDPAILSAVGA
                     GLTAA"
     gene            4626..4826
                     /gene="thiS"
                     /locus_tag="PLP58_19355"
     CDS             4626..4826
                     /gene="thiS"
                     /locus_tag="PLP58_19355"
                     /inference="COORDINATES: protein motif:HMM:TIGR01683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur carrier protein ThiS"
                     /protein_id="HRJ10747.1"
                     /translation="MTIILNGEKRGFDGPMPLPSLLEKLGLAGRPVVVEHNQSALLPR
                     EIESALVADGDVIEIVQITAGG"
     gene            complement(4986..6212)
                     /locus_tag="PLP58_19360"
     CDS             complement(4986..6212)
                     /locus_tag="PLP58_19360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009961586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HRJ10748.1"
                     /translation="MSRKIRYGMIGGGRGAFIGGVHRIAAAIDQQTELVCGAFSSNPE
                     KSKASGADFFLPADRCYGTFEEMIQKEAALHADKRMDFVSIVTPNHMHFPAAKAALEA
                     GFHVLSDKPATFNLAQAKELAEIVKKSGKLYGVTYNYTGYPMIRQARQMIEQGKLGKI
                     RKVVAEYPQGWLATMLENTGQKQASWRVDPAICGAAGCMGDIGTHAINLAEYVTGLHI
                     KELAADLTHFLGDQGRKLEDDGNVLLRFTNGAKGVLHSSQISVGCENNLSLRVYGELG
                     GIEWNQLDPNTMIVTWLDQPKQIYRTSMGYLGTAAAAATRTPAGHPEGYLEGFANLYK
                     NFVNHIRAVEAGATPNPLDMDYPTIEEGVSGMAFIEAVVASSANNAAWTPLDFTPTPR
                     PKYGDATLNSDRGGLS"
     gene            6350..>6907
                     /locus_tag="PLP58_19365"
     CDS             6350..>6907
                     /locus_tag="PLP58_19365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007415167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P1 family peptidase"
                     /protein_id="HRJ10749.1"
                     /translation="MKLLLGVLACLAATVFAGDKRARELGIEPGVMRPGALNAITDVP
                     GVRVGHATLIEGSGVRTGVTAILPHEGNPFLEKVPAAIHAGNGFGKLAGATQVEELGN
                     LESPVILTNTLAVGTAVSAVVENLLGLEGMEEVRSINAVVGETNDGGLNDIRSLPVRR
                     EHVWQAIASAAPGPVAEGCVGAGAGT"
BASE COUNT         1119 a         2265 c         2296 g         1227 t
ORIGIN      
        1 ctccgcgccc gccgctcatc ccgcgcatga gtccttcccc ggcaaagcgc ccgccgccgc
       61 gccgcgcgtg agccgcgagg tgtctgtcaa tccggccatc cccggctgga ccagcaccgg
      121 tctttacgcc gccgccggcg agcctgtccg cgttcagatt ccggcggagc gcgcgggcaa
      181 gggctacgcg ctgcgcatcg gctgccacag tgacacgctt tatcatctcg actcctggct
      241 gcgcgcgccg gagatcacca aaagcgtgtc tctcgacatg gcggagacga cggtgtccag
      301 cgcctttggc ggcctcattt acatcgtcgt gccggacaag gcagcggacg ggacccgctt
      361 cacctgcgcg ctgcatggcg gcatcccggc gccgcatttc gtgctgggtc tgcacagtga
      421 cgcgcaatgg gcgggcgaga tcagcaggca tcccgcgccc tgggcggagt ttgaatgcgg
      481 aaacatcatc ctctcctgcc cggcggacac cgcgcgcgcc gtgcgcaatc ccgcccagct
      541 catggagttt tggaaaaagg tcgtcgaagc gcaggacgag atcaccaacc aggccgccga
      601 gcggcgcagg ccggagcgca tcgtcgccga tgtgcagatc agcgccggct acatgcacag
      661 cggctacccc atcatgatcc ccgtgagcgc ggcggaggag atggtcacat tcaaccggct
      721 gaaatttccc ggctgggggt tttatcatga gatcggccac aaccaccagc gcccgcattt
      781 caccttcgag ggcaccggcg aggtgacgaa caatgtcatc agcatgtggt gctacgaggc
      841 cgtgctgggc aaggactggc tcattggcca tcctaacatc accgcggaag cgcgcgcgga
      901 gcacatccag aaaatcaagc gcgcctccga caagtgggcc gtgtggaaaa gcgatccctt
      961 cctcgccctc accacctaca tccagctcgt gcagggcttt ggctgggact cctggcgcaa
     1021 gtacctccac agcttcgccg acccagccca cggccccatg cccgccggcg acgacgagcg
     1081 ccgggaccag ttcctcgtgc gctactccaa aatcgtgaac cgcgacctcg gccccttgtt
     1141 tgacttctgg ggcatccccg tgagtcccgc cgccaaggcg agcgtggccg gactggaacc
     1201 gtggatgccg aaaatgtagg ctcgggcgac gccgccggag tgaggctatc aggcggtcgg
     1261 aaaggccgcc tcgcggtgaa tgccatccca tcaagctcga accaccccac cgctctcttg
     1321 cggcagccgg gcctcctgtt aaagtcgcac catcatgaac aaaaatcctt tcccaggcat
     1381 gaaccccttc atggaggcgc tgtggccgga tattcacaca gcactcatcg gttacattcg
     1441 cgacagcatc gccgagcagc ttcctcccga ccttaacgcc cgctccgagg aaagcgtgac
     1501 cctgcatgac gacctcaata cacccccgtc caaggcgcgc gcggatgtgg ccgtcacgga
     1561 gtcctggcgg cagggcatcc cgccttcctg gaatcccgcc caggaaacgg acggtccggc
     1621 gcggacactc tcccgtccgc aaatcatcct ggtcgaggag gaggtggagc gctggatcga
     1681 gatcaccgac cggcatggcc tcctgatcac cgtcattgaa gtgctcagcc ccgcaaacaa
     1741 aggcgcccac cgcgaggaat accgcagcaa gcgcgatgcc ttccttcacg ctggcgtcaa
     1801 tgtcgtggag atagacctcc tgcaccaggg caggcacacg gtggccgtgg cccttgaatc
     1861 cgtgcgcatg agcgctcccg gccagcatta cctgatccgc gtcacccgcg ccacccgccc
     1921 ttccgcgcgc gaggtctatg ccgccccgct gcgcgagcct ttgcccgtca tctccatccc
     1981 gctgcgcgcg aaggacaagg acgccgtgct ggctctgcag cccctggtgg accgctgcta
     2041 ccgcatgggt gcctactgga acgccaacca cgacaccctt cccgaacccg ctcccgcgcc
     2101 cgatgatgag gagtggatac gctcgcgggt caaagcggcg cgcgaacaag cggcggcttg
     2161 agagtccgcc gccacctcac ttgccggcaa accaactctt cgcgagcgga tcggtggcga
     2221 agaactccag cagcaggcgg gccaccttct ctcgtccgct gttgctggga tgcacgccat
     2281 ccgcggcgaa gtcatccggc gcgtacttca acccatccag cttgcgtccc ttctccccct
     2341 ccgcccagag gtagggaccc caaagcagca ggggcgcgcg gccggcggcc agggagtcgt
     2401 caccgctcat ctgcctgcgg atcaggtttc gcgccgcgaa ggcgctttca taggcgtagg
     2461 gttcggggtt cagagcccct ctggcattgc ccgcccagat gcggctgccg agataggcga
     2521 cgcggaggtt cgggaagcgc tcgcgcgcgt tgcgcagcac ggcggtggtg tcggcttcca
     2581 gcctggccag atgctcgctc atgccgccag aggggctttt gttcgccagc ttgatccacg
     2641 ccacctgcac ctgcgcggca gtgactccgg ccgcgctgat gcggcgcatg gcttcctccc
     2701 acggcctgcc cgctggcggc gcccactccg ccatcgcctg gccgccctgg gcgcagtcca
     2761 cgatggtgag tttggcgaac tttcgcgggt ccgcgtcggc gatgcgtttg aaaacggaga
     2821 actcctgcgt ggcgtttgac atgctgatgg agaccagcac gaccctgccg tcgtccgccg
     2881 gtttgccctc tcggttcaga ggctggattt gcgccagcgc ggccgccgcc gttttcctca
     2941 aggcctccgg cggttcgttc atgccgccgc cgtacaagcc gccgtcctgg ccttcatagc
     3001 ggtcctccgc ggtcatgtcg cacagaggcc ggagtgactc gggcgccttg cgctggcctg
     3061 ctcccgcctc gggccgggcc gcgcctttcc tgcgcgcctc cttggcgcgg tccaggtatt
     3121 tttgatcctc ggcagccagc ttctcaccgc gctgctcgcg ctggaagagc tgtctggcgc
     3181 gatcccagtc aatcggggtt tcggcctcct ggccggggaa ggacaccgcg ttgaaaagca
     3241 gcgccgccgc gagaatggat gtgcggatgt tcatggcagg gcggggaaac acctccaaat
     3301 cacccgggtt gcactgccat cccccgaatg gtcgcacacc ggggcatgac ataggagcga
     3361 gccatccttg aaaagacggg tttcaaaagg atgcatttcg agtttccttt ctttgcttcc
     3421 tgcacaagcg tttccaccca ccaccacgct gatgcctgcg gattttctca ccacccttga
     3481 tgccctcccg gaccggaagt cgccgctcat ggagcgcttc tggtccatga tcgggccgaa
     3541 agcgcccgcg cggatcgagg ccatggcgcg cgaggcgcgg gcggtgacgc ggcggaactt
     3601 cggacgcacg atgcggcttt tcgccccgct ttacctttcg aacgagtgcg tgaacaactg
     3661 cgcctactgc ggtttttccc gcgacaaccc ggacatcctg cgcgtgacgt tgagcgtgga
     3721 ccaggtggtg cgcgaggcgc ggcacctggc ggagcagggc ttccgcaatg tgctgctggt
     3781 ggccggggag cacccgcgtt ttgtcagcga gggctatctg gaggagtgca tccgcgcgat
     3841 tcgcggtttc atacccaccg tgggcatcga agtcggcccg atggaggcgc ccgagtatgc
     3901 gcgcatggtg gaggctgggg ccgaggggct ggtggtgtat caggagacct acgaccgcga
     3961 agcctatgcg gaaatgcaca ccgccgggcc gaagaaggac ttcgcctggc gcatggcctg
     4021 cccggagcgc ggacatgagg gcggcttccg ccgcatcggc atcggcgcgc tcttcggcct
     4081 ctccgactgg cggctggagg cgctgcggct ggcggcgcat ctggagcatc tgctgcggca
     4141 ctgctggcgc gccagtttca cggtggcctt tccgcgcctg cggccggcgg cgggcggatt
     4201 ccagccgctg acggctttcc cggactgggc gctggtgcag acgctgtgcg ccttccgcct
     4261 caccttcccc gaggccggca tcgtgctgag cacgcgggag cctgcggcgc tgcgggatgc
     4321 cctggcgccg ctgggtgtga cgagcatgag cgcgggcagc cacaccgagc ccggcggcta
     4381 caccggcgcg ggcgcggagg acctgcacaa gacggtcaaa ggccgccgcg tggacctgga
     4441 ggataaaccc tgctcatcgc gcgcggaggg ccagttccac atcgccgacg aacgctcccc
     4501 ggcggaagtc gccgccatgc tgcgcgcgcg ggggctggac ccggtgtgga aggactggga
     4561 ccccgccatc ctctcagccg tgggcgcggg cttgacggcg gcgtgaggct tttttcactc
     4621 catccatgac gatcatcctc aacggcgaaa aacgcggctt tgacggcccg atgcccctgc
     4681 cctccctgct ggaaaaactc ggcctggccg gcagacccgt cgtggtcgag cacaaccagt
     4741 ccgccctgct gccgcgcgag atcgaatccg ccctcgtcgc ggacggggat gtcattgaga
     4801 tcgtgcagat cacggccggg ggatgagccg gggagggggt tcctccagac ttcactgtcc
     4861 ctgtcagatc tgcttccctg agacgagcag gcggcggcag cttgaattca tcccaaatat
     4921 ttcggggaga acggcacgcg gacgttctcc ccgaatcgtt ttctgatgat gatgtcgtcc
     4981 gtgtttcagg acaacccgcc cctgtcgctg ttcagcgtgg cgtccccgta tttcgggcgc
     5041 ggagttggcg tgaaatccag cggggtccag gcggcgttgt tggcggagga ggccaccacg
     5101 gcttcaataa aggccatgcc gctcacgcct tcctcgatgg tggggtagtc catgtcgagc
     5161 gggttcggcg tggcgccggc ctcgacggcg cggatgtggt tgacgaagtt tttgtacagg
     5221 ttggcgaagc cttcgagata cccctcgggg tgcccggcgg gagtgcgggt ggcggcagcg
     5281 gcggcggtgc cgaggtagcc catgctggtg cggtagattt gcttgggctg gtcgagccag
     5341 gtgacgatca tggtgttggg gtcgagctga ttccattcga tgccgccgag ctcgccatag
     5401 acccgaaggc tgaggttgtt ttcgcagccg acgctgatct ggctggagtg cagcacgcct
     5461 ttggcgccgt tggtgaagcg caggagcaca ttgccgtcgt cctcaagctt gcggccctgg
     5521 tcgccgagga agtgggtgag gtcggcggcg agctccttga tgtgcaggcc ggtgacgtat
     5581 tcggcgaggt tgatggcatg ggtgccgatg tcgcccatgc acccggcggc tccgcagatg
     5641 gcggggtcca cgcgccagga ggcctgcttt tggccggtgt tctccagcat ggtggccagc
     5701 cagccctgcg gatactcggc cacgactttg cggatcttgc ccagcttgcc ctgctcgatc
     5761 atctggcgcg cctggcggat catcgggtag ccggtgtagt tgtaggtgac gccgtagagc
     5821 ttgccggact tcttcacgat ttccgcgagt tccttggcct gggcgaggtt gaaggtggcg
     5881 ggcttgtcag aaaggacatg gaagcctgcc tcaagagccg ccttggccgc cgggaaatgc
     5941 atgtggttcg gcgtgacgat ggagacaaag tccatgcgct tgtcggcgtg gagcgcggcc
     6001 tccttttgga tcatctcctc gaaggtgccg tagcagcggt cggcgggcag gaagaaatcg
     6061 gcgccgctgg ccttggattt ttccgggttg gatgaaaacg cgccgcagac gagttccgtc
     6121 tgctggtcaa ttgccgccgc gatgcgatgg actccgccga tgaaggcgcc gcgtccgccg
     6181 ccgatcatgc cataacgaat tttccggctc atataaaagt gggtgtgggg ttggtggtga
     6241 aagagggccg aaactgcgca gagccgccgc gcgggtcaag cgcgcgtttg acggaagcgc
     6301 gttgacgaag ccgttggcgg aacgggcgcg gcgtgtcaat gtgcgcgcca tgaagcttct
     6361 cctcggagta ctcgcatgcc tggccgcgac cgttttcgcg ggggacaaac gcgcgcgcga
     6421 gctgggcatc gagcccggcg tgatgcgccc gggcgcgctc aatgccatca ccgatgtgcc
     6481 cggcgtgcgc gtgggacacg ccacactgat cgagggctcc ggcgtgcgca ccggtgtcac
     6541 cgccatcctg ccgcatgagg gcaatccttt tctcgaaaaa gtccccgccg ccattcatgc
     6601 cggcaacggc tttggcaagc tggctggcgc cacgcaggtg gaggagctgg gcaatctgga
     6661 gtccccggtc atcctgacca acacgctggc ggtgggcacg gctgtgagcg cggtagtgga
     6721 gaatcttttg ggactcgagg gcatggagga agtccgctcc atcaacgcgg tcgtcggcga
     6781 gacgaatgac ggcggtttga atgacatccg ctcccttccg gtgcggcggg agcatgtgtg
     6841 gcaggccatc gccagcgccg cgccagggcc ggtggcggag gggtgcgtcg gcgcgggcgc
     6901 gggcacg
//
LOCUS       DAOMYJ010000258         6857 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Prosthecobacter sp. isolate AMR_MDS_5629
            NODE_3761_length_6857_cov_18.185387, whole genome shotgun sequence.
ACCESSION   DAOMYJ010000258 DAOMYJ010000000
VERSION     DAOMYJ010000258.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867850
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prosthecobacter sp. (wastewater metagenome)
  ORGANISM  Prosthecobacter sp.
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Verrucomicrobiaceae; Prosthecobacter.
REFERENCE   1  (bases 1 to 6857)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6857)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.30
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:34:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,742
            CDSs (total)                      :: 4,684
            Genes (coding)                    :: 4,676
            CDSs (with protein)               :: 4,676
            Genes (RNA)                       :: 58
            tRNAs                             :: 55
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6857
                     /organism="Prosthecobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3761_length_6857_cov_18.185387"
                     /isolate="AMR_MDS_5629"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1965333"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..631
                     /locus_tag="PLP58_19370"
     CDS             <1..631
                     /locus_tag="PLP58_19370"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HRJ10750.1"
                     /translation="NEPTATPGGPEQLARQLAGHGSIRQWLLVMGKLGPRKRQVDAVR
                     VLRHLLDSGHHGLGLLLAGDCEPDYEPLMQAAIRDCRAQDRVLMLGNVQDVPALLALR
                     PVCLLTSLREALPGSVIEALGSGRPCFMYPCEGAEDIFGPHLEHFVSAGFQPALLAEK
                     MHALLSDPPRLESETAALQARARSLFSQEAHLATCLATFGLDPRPASGA"
     gene            complement(1656..2333)
                     /locus_tag="PLP58_19375"
     CDS             complement(1656..2333)
                     /locus_tag="PLP58_19375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="PEP-CTERM sorting domain-containing protein"
                     /protein_id="HRJ10751.1"
                     /translation="MRISIASALFLAAASFGHASITINFGLGGVYTAGGGSQIAAGTI
                     GVLVADTTGAGFAQGADLVGSTLSVGSSLGTNGRILGVLSATNNLFGSGIAGFDAAIN
                     ITDPLLSGLTFGTATNNAGSDLMFYWFPGITSAGSTITEGQSYGSFREDSLAALAGSA
                     SDMSFNLPANSDLLGLYANTINTGGDLPNDTFQANSTAGVAGVPEPSRMILALLGFAG
                     LMFRRRR"
     gene            complement(2384..3451)
                     /locus_tag="PLP58_19380"
     CDS             complement(2384..3451)
                     /locus_tag="PLP58_19380"
                     /inference="COORDINATES: protein motif:HMM:TIGR02597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02597 family protein"
                     /protein_id="HRJ10752.1"
                     /translation="MKASISTCLSLLLCLGAGSLYSQSAETNPVGFNTVTCLPNSDTI
                     CGVPFARPAVFQGVVVSTQAGTGTLTITPDGTPGWTENAFQTLYMVRIISGAKAGMYY
                     EVSANAVGDVTVNLAGDTATGINPGDSFRIHPFWTLSTLFPPGNQNTIVESLSTAPAG
                     RRTQILLPDVNGVGFNLAPLQTFFLVTGGAGWKATGGVDATNEILFPDTYFIVRHNHA
                     TITNSTTFTPVGSVELNDIVTPLATRSAGAQDNFVSSGRPIPVKLSDLDLLSSGAFVQ
                     SLSLSPGGRRDLLLVFDNSVSGINKAPAVTCFANGGNWYVIDSTGTNLNNDYEIKPSH
                     GVIIRKYANAGSVTSYWKNSF"
     gene            3637..4752
                     /locus_tag="PLP58_19385"
     CDS             3637..4752
                     /locus_tag="PLP58_19385"
                     /inference="COORDINATES: protein motif:HMM:NF014460.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRJ10753.1"
                     /translation="MPDSASTFPPRASWQQGGRVLRLEGSWHRDAPAPVIEGSPPAAM
                     PERYEAEGLRGFDSTLPAFLLAHARTAGGAAAGPWQGLPENLRGLMELALSVPEKDDA
                     RSGPQKKSGLRELGRRTLQVWQAARDAVEFTGESVLALGRFATGRARFRARDFWEVLQ
                     QCGAEALPIVALISFLVGLILGFVGNVQLTNFGANIFVADLVAIAMVREMGVMMTAII
                     MSGRTGAAFAAHLGSMKANEEIDALRTFGLQPFDFLVLPRLLALVLMMPLLTVYSNVI
                     GILGGMVVGISVGIPPVLYWNETLLSLTMTTASLGVMKSFFFGAAIAICGCMTGMNAG
                     RSSAAVGEATTRAVVASITVVIVLDSAFAAIFTILDI"
     gene            4763..5554
                     /locus_tag="PLP58_19390"
     CDS             4763..5554
                     /locus_tag="PLP58_19390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007414963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HRJ10754.1"
                     /translation="MSAAAQPPKARISARSLTMAYGSFVVMRDLEFDIRERDIFIIMG
                     GSGCGKSTLLRHLIGLREPAQGEVFYDGESFTRAGEAERERLTRRFGVMYQSGALWSS
                     LTIAENVALPLEEFTTLPARTVRDIVSYKLSLVGLAGFEDFYPSQVSGGMNKRAGIAR
                     AMALDPDILFLDEPSAGLDPLSARRLDDLILRLRDSLGSTFVIVTHELASIFAIGSNS
                     IFLDTDTRAQGAIGPPCFLRDHSESATVRRFLNRGEEGAPGKPAS"
     gene            5558..6577
                     /locus_tag="PLP58_19395"
     CDS             5558..6577
                     /locus_tag="PLP58_19395"
                     /inference="COORDINATES: protein motif:HMM:NF014523.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MlaD family protein"
                     /protein_id="HRJ10755.1"
                     /translation="MSRKASPTLIGVFTLLALLFAAGAIMLLGAGKFMQRTHRILMHF
                     EKSAMGLLVGSDVRFGGVRIGRVADISVLVDHKNNRKVIPVIVELEEKDLRSIRSTSG
                     NALDFTTPEGVVKAVKDGLRAGMVQQSLLTGMLYIEFDIVPDEPGFVYEDEDGHELPT
                     VPTIPTEIDQLVAGVADGLKKFNALDIDGVVKDLQKLLRDADEQIVALNMREISENFV
                     AVSKDVRAFTGNAKITSAFEKLDSALAELRELSAKLNKGMDPVLADAAKVLEKAQAGM
                     DSLGKAAADISQASNPRGPVLMRLQMVLDETERASRAIKELANDLKRNPNAILVGKEQ
                     QEPQR"
BASE COUNT         1260 a         2188 c         2255 g         1154 t
ORIGIN      
        1 gaacgaaccc accgccacgc ccggcgggcc ggagcagctc gcccgccagc ttgccgggca
       61 cggcagcatc cgccagtggc tcctggtcat gggcaagctc ggcccccgca agcgccaggt
      121 ggacgccgtc cgcgtcctgc gccacctgct cgactccggg caccacggcc tcggcctcct
      181 gctcgccggc gactgcgagc cggactacga gccgctcatg caggccgcca tccgcgactg
      241 ccgcgcgcag gaccgcgtgc tcatgctcgg caacgtccag gacgtgcccg ccctcctcgc
      301 cctgcgcccc gtctgcctcc tcaccagcct gcgcgaggcc ctccccggct ccgtcatcga
      361 ggcgctcggc tccggccgcc cctgcttcat gtacccgtgc gagggcgccg aggacatctt
      421 cggcccccac ctcgagcact tcgtcagcgc cggcttccag cccgccctcc tggcggagaa
      481 aatgcacgcc ctgctttccg atcccccccg gctggagtcc gagaccgccg ccctccaggc
      541 ccgcgcccgc tcccttttct cccaggaggc ccacctcgcc acctgcctcg ccaccttcgg
      601 cctcgacccc cgccccgcat caggcgccta gaaagaagca gcgcgcctcc ccggcgcgaa
      661 cccgcccgcc caggggcatc cgcgcgcctc tcccgatggg actcatgccc ggatccagcc
      721 cggccccgga ccatccgcgg ccaatcccca acccgcgccc cgccgcccag ccaccctgga
      781 gacgccccgg cagacttccc gccgcattcc gcgcgttttg gaggcgcgcc accaccccct
      841 tcagcgcatt ccgcgcgttt tggaggcgcg tcaccacccc cttcagcgca tcccgcgcgt
      901 tttggaggcg cgccaccacc tccttcagcg catcccgcgc gttttggagg cgcgtcacca
      961 aaccccccgg aagattccgc gcggtttggt gacgcgtctc caaagtctgg agatgcgtca
     1021 ccaaagtctg gagacgcgtc tccaaagcgt ggtgaagccg ttttcaaacc gagaaaaccc
     1081 gtcaccaaag cccggagacc cgtcaccaaa gcgtggaggc gcgtcaccaa agcgtggtga
     1141 actgtctcca aagcctggtg aaccgtcacc aaagcgtgga aaaccgtctc caaagcccgg
     1201 tgaaccgtca ccaaagcgtg ggaaaccgtc tccaaggcgc gggaaaccgc caccaagacc
     1261 gggtgaatcg tccccaaagg ccctaaaaca cgcccaaaag agccgaaaaa ccgccaatat
     1321 cgaaaaacgc ccgctttccg ggcaacacgc cccacctggg agcgccaggg cggtctgtcc
     1381 ccagcccccc cgcgcgctgg cgcgcagtgc gagcggcgcg gcagggtggt tcgtaccctc
     1441 ctccccggca cgcggccgtt gaggacaacg cgccccaccc tgcgccgcag gcgcgccttc
     1501 cggggcaggt ccggctggtc ctccaccccg cgcgcccaag gccctgtgtc cgggggtggg
     1561 aaaagaccgg gcggcagggc gttcccggcg caaaaaaaag cggccccgaa tcacttcggg
     1621 gccgcccagg gaaaaaacac aaactcaaac ccgcctcaac ggcggcgacg gaacatcagc
     1681 cccgcaaaac ccagcagcgc cagaatcatc cggcttggct ccggaacacc ggcgacaccg
     1741 gctgtggagt tggcttggaa ggtgtcgttt ggaagatctc caccagtgtt gattgtattg
     1801 gcgtagaggc caagaagatc actatttgca ggcaaattga aggacatatc agaagccgaa
     1861 cctgccaggg cagccagact gtcttcacgg aacgagccgt atgactggcc ctcagtgatc
     1921 gtgcttccgg cagaagtgat gcctgggaac caatagaaca tcaggtcgga acctgcattg
     1981 ttggtggctg tgccaaaagt cagaccggaa agcagggggt ctgtgatatt tattgccgcg
     2041 tcaaagcccg cgatgccgga accgaacagg ttgttcgtgg cagagagtac gccaagaatt
     2101 cgtccattcg ttccgagact cgagccaaca gagagggtgg aaccaaccag atccgcgcct
     2161 tgcgcgaaac cagcgccagt ggtgtccgca acgagcacac cgatggtgcc tgcggcgatc
     2221 tggctgccgc ctcccgccgt gtaaactccg ccgagcccga agttaatagt gatggaggca
     2281 tgcccgaagg atgccgcagc aaggaaaaga gcgcttgcga tggagattct cattggagtg
     2341 tgtgttgtga ggttgcgtta aggatttgaa aaggagaatg gcttcagaag ctatttttcc
     2401 agtagcttgt gacgctgccc gcattggcat atttgcggat gatgacgcca tgagaaggtt
     2461 tgatctcata gtcgttgttt aagttcgtgc cggtagagtc aatcacatac cagttgccac
     2521 cgtttgcaaa gcaggtgact gcaggagcct tgttgatgcc ggatacgctg ttgtcgaaga
     2581 cgagcagcaa atcccgcctg ccgccaggag agaggctgag actctgcaca aaggcaccgc
     2641 tgctgagcaa atccaaatcc gagagtttga cgggaatggg ccgtccagaa ctgacgaaat
     2701 tgtcctgggc gccagcggaa cgagtggcga gaggcgtgac gatgtcgttc agttcgacac
     2761 taccgaccgg agtgaaagtt gtcgaattgg tgattgtggc atgattgtga cggacgatga
     2821 aataagtgtc cgggaaaagg atttcattgg tggcatcaac acctcccgtt gctttccatc
     2881 cagcaccgcc agtaacgagg aaaaatgtct gcaagggagc caaattgaag ccgacaccgt
     2941 ttacgtcagg cagcaaaatt tgagtgcggc gacccgccgg agcggtgctc agcgattcga
     3001 caatcgtgtt ttgatttcca ggcgggaaca gagtcgaaag cgtccagaag gggtggatgc
     3061 ggaaggagtc gccggggttg atgccggtgg cggtgtcacc ggcaagattc acggtgacgt
     3121 cgccgacggc gttggcggag acctcataat acatgcccgc cttggcgccg gagatgatgc
     3181 ggaccatgta gagggtctgg aaggcgtttt cggtccagcc gggggtgcca tccggggtga
     3241 tggtgagggt gccggtgccg gcctgggtgc tgaccaccac gccctggaac acggcgggac
     3301 gggcgaaggg cacgccgcag atggtgtcgg agttcggcag gcaggtgacg gtgttgaagc
     3361 cgacgggatt ggtctcggcg gactgggaat aaaggctccc tgctccgagg cagaggagca
     3421 ggctgaggca ggtggagatg gaggctttca tggcgggatg gaatgttttc acattatttt
     3481 accctttttc agggctgttg ttaccgggtt tgcgagattt ggtcgagatt tttttcggag
     3541 gcggcgattt tttttgaaac tggccgggct tcagggggtg ccgcgggcgc ggaagcgctc
     3601 cttgccgccg ggggcttccg gtggcatgga gggcgcgtgc cggactccgc ctccaccttc
     3661 ccgccacgcg cctcctggca gcaggggggc agggtgctgc gcctggaggg aagctggcac
     3721 cgggacgcgc cggcgccggt gatcgagggc agtccgcccg cggccatgcc tgagaggtac
     3781 gaggcggagg ggctgcgggg gtttgactcg acgctgccgg cttttttgct ggcccatgcg
     3841 cgcacggcgg gcggggcggc ggcgggtccc tggcaggggc tgccggaaaa tctgcgcggt
     3901 ctgatggagc tggcgctgag cgtgccggag aaggatgacg cgcgctccgg gccgcaaaaa
     3961 aagtccggtc tgcgggagct ggggcggcgc acgctgcaag tctggcaggc ggcgcgggac
     4021 gcggtggagt tcacagggga gtcggtgctg gcgctgggcc gcttcgccac cgggagggcg
     4081 cggttccggg cgcgggattt ctgggaggtg ctccagcagt gcggggcgga ggcgctgccg
     4141 atcgtggcgc tgatcagctt cctggtgggg ctgatcctgg ggttcgtggg caatgtgcag
     4201 ctcaccaact tcggcgcgaa catcttcgtg gccgacctgg tggcgatcgc gatggtgcgc
     4261 gagatggggg tgatgatgac ggcgatcatc atgagcgggc gcacgggcgc ggccttcgcg
     4321 gcgcacctgg gcagcatgaa ggcgaacgag gagatagacg cgctgcgcac cttcggcctc
     4381 cagccctttg actttctggt gctgccgcgc ctgctggcgc tggtgctgat gatgccgctg
     4441 ctgacggtgt actcgaatgt gatcggcatc ctcggcggca tggtggtggg catttccgtg
     4501 ggcatcccgc cggtgctcta ctggaatgag acgctgctct cgctgacgat gaccacggcc
     4561 agcctggggg tgatgaagag ctttttcttc ggcgcggcca tcgccatctg cgggtgcatg
     4621 acgggcatga acgcgggccg ctcctccgcc gcggtgggcg aggcgacaac acgcgccgtg
     4681 gtggcctcca tcaccgtggt gatcgtgctg gactcggcct ttgccgccat cttcaccatc
     4741 ctcgacatct gactccgccc ccatgagcgc cgccgcccag ccaccgaaag ccaggatctc
     4801 cgcgcgcagc ctgaccatgg cctacggctc cttcgtggtc atgcgggacc tggagttcga
     4861 catccgggag cgggacattt tcatcatcat gggcggcagc ggctgcggca agagcaccct
     4921 cctgcgccat ctcatcggcc tgcgggagcc ggcgcagggg gaggtgtttt atgacgggga
     4981 gagcttcacc cgagccggcg aggcggagcg ggagcggctg acgcggcgct tcggggtgat
     5041 gtatcaatcc ggcgcgctgt ggtcctcgct caccatcgcg gaaaatgtgg cgctgccgct
     5101 ggaggagttc accaccctgc ccgcgcggac ggtccgcgac atcgtctcct acaagctctc
     5161 gctggtgggg ctggcgggtt ttgaggactt ttaccccagc caggtcagcg gcggcatgaa
     5221 caagcgcgcc ggcatcgcgc gcgccatggc gctggacccg gacatccttt tcctcgacga
     5281 gccgagcgcg ggcctcgacc cgctgagcgc gcggcggctg gacgacctga ttttgcgcct
     5341 gcgtgacagc ctcggctcca ccttcgtcat cgtcacccac gagctggcca gcattttcgc
     5401 catcggcagc aactccatct ttctcgacac cgacacgcgc gcgcagggcg ccatcggacc
     5461 gccttgtttc ttgagagatc acagcgagag tgccaccgtg cgccgctttc tgaaccgcgg
     5521 ggaggagggc gcgcccggca agcctgcatc ctgactcatg agccgcaaag ccagtcccac
     5581 tctcatcggc gtcttcacgc tgctcgcgct gcttttcgcc gcgggagcga tcatgctgct
     5641 gggcgcgggg aagttcatgc agcgcacgca ccgcatcctg atgcacttcg aaaaaagcgc
     5701 catgggcctg ctggtcggct cggacgtgcg gtttggcgga gtgcgcatcg gccgcgtggc
     5761 ggacatcagc gtgctggtgg accacaagaa caaccgcaag gtcatcccgg tcatcgtgga
     5821 actggaggag aaggatttaa ggtccatccg ttccacctcg ggcaatgctc tcgacttcac
     5881 caccccggag ggagtggtca aggccgtgaa ggacggcctg cgcgcgggga tggtgcagca
     5941 gagcctgctg acggggatgc tttacatcga gttcgacatc gtgccggacg agccgggctt
     6001 tgtgtacgag gacgaggacg gccacgagct gcccaccgtg ccgaccatcc cgacggagat
     6061 agaccagctc gtggcggggg tggcggacgg attgaaaaag ttcaacgcgc tggacattga
     6121 cggcgtggtc aaggacctgc aaaagctgct gcgcgacgcc gacgagcaga tcgtggcgct
     6181 gaacatgcgg gagatcagcg agaatttcgt ggccgtgtcc aaagacgtgc gcgccttcac
     6241 cggcaacgcc aagatcacct ccgccttcga gaagctcgac agcgccctgg ccgagctgcg
     6301 cgagctgagc gccaagctca acaaaggcat ggaccccgtg ctggcggatg cggcaaaggt
     6361 gctggaaaaa gcccaggccg gcatggacag cctcggcaag gccgccgccg acatctccca
     6421 ggccagcaac ccgcgcggcc ccgtgctcat gcgcctgcaa atggtgctgg acgagaccga
     6481 gcgcgcctcg cgcgccatca aggagctggc caacgacctc aaacgcaacc ccaacgccat
     6541 cctcgtcggc aaggagcagc aggaaccgca gcgatgaaac acgaagcgac aactgggaaa
     6601 ttcgacatgc gaaattcgaa gctcgaacag cggggcggga cttgggctga tccccgtagg
     6661 cgcgcttgcc agagcgcggg gacggctcac gcacacggag ccgattcccg cattctggcg
     6721 aatgcgccta cagtgccgca gaggcgcgct tgccagggcg ttttcgttgc gctgacggcg
     6781 ttttgcttcg cctcctgctc cctcaccaag ccggtgagtg accccgccac gcggcatgtg
     6841 ctggacccgg ccatccc
//
LOCUS       DAOMYJ010000259         6796 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Prosthecobacter sp. isolate AMR_MDS_5629
            NODE_3813_length_6796_cov_19.319982, whole genome shotgun sequence.
ACCESSION   DAOMYJ010000259 DAOMYJ010000000
VERSION     DAOMYJ010000259.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867850
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prosthecobacter sp. (wastewater metagenome)
  ORGANISM  Prosthecobacter sp.
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Verrucomicrobiaceae; Prosthecobacter.
REFERENCE   1  (bases 1 to 6796)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6796)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.30
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:34:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,742
            CDSs (total)                      :: 4,684
            Genes (coding)                    :: 4,676
            CDSs (with protein)               :: 4,676
            Genes (RNA)                       :: 58
            tRNAs                             :: 55
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6796
                     /organism="Prosthecobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3813_length_6796_cov_19.319982"
                     /isolate="AMR_MDS_5629"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1965333"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..731)
                     /locus_tag="PLP58_19400"
     CDS             complement(<1..731)
                     /locus_tag="PLP58_19400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ10756.1"
                     /translation="MADNPIETAAQELKKWLEQLQKIYQLKIDEEKKAQALAKEQAKE
                     ALAAERHEEMMEELRGIREGKSEGQGKDYSAPYEDLDEDWEPPEPEVEYDYEKDVKEA
                     REFKAKLNEMPDGDDKAQLLREKGPEARKMFDDYRKQVEARPDCLGKEALLKELDDLD
                     QMLDYEAYAQEHGLDGNELDALDGADDLDAGDMGMDAPEIGDPGEGLEQAVEGIEQAA
                     DQAGEALEQASKGVQQAAGAVAGGAG"
     gene            complement(758..1198)
                     /locus_tag="PLP58_19405"
     CDS             complement(758..1198)
                     /locus_tag="PLP58_19405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ10757.1"
                     /translation="MHHTPVASSSLRRCWQALALIAFSLLPSCQSGPMPLGDAQLLPP
                     GTLVEFWVYRDAKNEDGEEIGWQKSNIHVLSLPRPMSGKEARLWAEGVGRRDAGMRWT
                     HYYVLATPPKPAYRPAHTYRSAVFPNRALPNEIIAARPGNTRNP"
     gene            complement(1223..1627)
                     /locus_tag="PLP58_19410"
     CDS             complement(1223..1627)
                     /locus_tag="PLP58_19410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ10758.1"
                     /translation="MGIWSSIREFWARHRRFLTRYGTGTLLFVSALAMGKIPVACLIL
                     GGILVIYAVDRWGEEHLLRGRMLMEQRRLEQQRKMQELKQAVGDFLGARAKQASSAPP
                     PAGKEEDEPVPGASFIHEPPPEPPKPPVTHET"
     gene            complement(1602..2498)
                     /locus_tag="PLP58_19415"
     CDS             complement(1602..2498)
                     /locus_tag="PLP58_19415"
                     /inference="COORDINATES: protein motif:HMM:NF019344.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HRJ10759.1"
                     /translation="MPDLQHIQGLPAFLKERIIGQDHVVPRVVPIIQNGELGLSDPGR
                     PKGTFLFLGPTGVGKTEITLLITEYIFKDVQKHCIRLDMSEYQNQESLGLLLGKDESS
                     RGNMGRFYDRAEGRGVILFDEIEKAHPRVLDIFLQVLDAGRITVASGETLNLCEFYIV
                     ATSNIGADALMELKNSPMATVERFIEDLAAAELRPEVLARFNVRCVFQKLGYAAQKQI
                     AQIMLNKELKLLRGKGYDLKAGPGVLDLLVAQGVEPRLGVRRMRATAEANCRHAVREA
                     LMAGRPTSGTLVAENGHLVIHP"
     gene            complement(2516..3211)
                     /locus_tag="PLP58_19420"
     CDS             complement(2516..3211)
                     /locus_tag="PLP58_19420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ10760.1"
                     /translation="MIPKLHHALLLALLVLTGASCRSKKTDLSDDPLVRPEDEQELAG
                     ITTYSKEVVQGWPRGRSLHPRDVSRVRLGEEVHAYHLGRLPSRDRREMHEAHTVYRVE
                     QDARWDTRLPATPMDSGGVILGITDPARDSLPKDILIAQERERLATMALNLQKSMARL
                     DELRRTLEKKSADFDAGKEEAVQIKQYLAEIRADRDEKEEQLKNAQERIKALEEQQRL
                     YDMTSQQSGARKP"
     gene            complement(3208..4422)
                     /locus_tag="PLP58_19425"
     CDS             complement(3208..4422)
                     /locus_tag="PLP58_19425"
                     /inference="COORDINATES: protein motif:HMM:NF015688.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrbI/VirB10 family protein"
                     /protein_id="HRJ10761.1"
                     /translation="MMKHLQKPGVQMALLFAVVAIGGAAYYLSTRPPPPVNVPPENET
                     PRTKGVGQMAVEEKKVEAGQQRTAEVSSDVSRLMLPKEKPKPPPALIETSSDSGPQKK
                     SGPPPLPRLVHVKNDIVEPHVPQPPSFFAPRGLLIKAALVITVDSSSLETPVLALVTE
                     DVYFKGRLIVPAGTQVLAQAAQGRSRDRIEVKGAYTFIWADGREYRINGVALDHERLP
                     DGTYGITDGSAGIRGQIVKADAYAELKILVAEALQGIMNNQQDQFQSIYGLAPQNTNR
                     NAALGGGSQAASAYSRMLTDRLAQDLDFVRVPAGTQFYIFTLDVFEPGLASVAGLVQG
                     EQPVSSWKLAEETYARAQAEQAALKQNAAAAEAQAGQVRAAQQALAPVSRVRGIVAGD
                     SAPSTPSTRSEP"
     gene            complement(4432..5343)
                     /locus_tag="PLP58_19430"
     CDS             complement(4432..5343)
                     /locus_tag="PLP58_19430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ10762.1"
                     /translation="MNTLLPALLHAQVKEGNEMVMLNPASAVHEIHINSHVATSVIFP
                     EPVSMLTGFGLVEDSAAVSGMAQSRTSVVHYELVMSDTLVVRLLKEGDPCHVTVRAGK
                     NIHLLRFAADVEAHLAVIIPPPGQRNTAREVAPVEVAQKRITYNSDELVGMLGRARQR
                     KFLQSVNPALYMGWQERNGLELSTAKDGVTCTIYEIQRWPEKDVLVFRCWLNNTGAAA
                     YEFDPVDIRVRADERAYAAQLVDCSGIVMPNEKIAMDVVLQGGPGGGREHLSIDNDFR
                     IDLPQAGRTRASAALFGGATATLDDGK"
     gene            complement(5388..6032)
                     /locus_tag="PLP58_19435"
     CDS             complement(5388..6032)
                     /locus_tag="PLP58_19435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ10763.1"
                     /translation="MSQAKPDITPKKRRGAVDAFVAKDRVAVFWFLIACLTAAACSWY
                     VVKVSDTMKERPPFVIMDGAGVFYSAPGLNFEKATPIHLALTEILVDTMLTRGPEGLA
                     YGRRLNKLCTRDALGQINKLIQEESSNFSRQQVRQTYEIEEVKSLGASGTAAGTQATG
                     RIFRRGQFGGAVEEVSTFKIKFQWVLNPDVRGNESFPALLFNLYSYERIPLSMP"
     gene            complement(6029..6643)
                     /locus_tag="PLP58_19440"
     CDS             complement(6029..6643)
                     /locus_tag="PLP58_19440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ10764.1"
                     /translation="MKGVSDLFISKEKLAFFWFFVCVAILAGTGWYVNDIAGDGKSRM
                     LYVMVNRPDVMYLDRDLQPETASDLFDTQTRLALETMLNRAPNGPVTQKRVAKIFGGP
                     ALEWLMKDLAENREEFRSRQYHQMAEVGSVRVFLGEDGGAIAMATGQVIRVGVDPVQQ
                     KVVNQVFNLGARLVWEPNINLRDNKLYPHTCREAAYTLTLVSES"
BASE COUNT         1099 a         2059 c         2250 g         1388 t
ORIGIN      
        1 cccgctccgc cagccaccgc accggcagcc tgctggacgc ctttgctggc ttgctccagc
       61 gcttctccgg cttggtcggc tgcttgttca atgccttcca cagcttgttc cagcccttca
      121 cctggatcgc cgatttcggg ggcgtccatg cccatgtctc cggcgtcaag atcatccgcg
      181 ccatccagcg cgtccaattc gttcccgtcc aagccgtgct cttgagcata tgcctcgtaa
      241 tcaagcatct ggtcaagatc atccaattcc ttcagcagcg cctccttgcc cagacagtcg
      301 ggcctggctt cgacttgttt tctgtagtca tcgaacatct ttcgggcctc aggccctttt
      361 tcccgcagca attgggcttt gtcgtccccg tcgggcatct cattgagctt tgctttgaac
      421 tcccgcgctt ctttgacatc tttttcgtag tcgtactcca cttcgggttc tggcggctcc
      481 cagtcttcgt ccaaatcttc gtagggtgct gaataatcct tgccctgtcc ttcactcttg
      541 ccctccctga tgccgcgcag ttcctccatc atttcctcgt gtcgctctgc ggcgagtgcc
      601 tctttggctt gctccttggc cagcgcctgg gcttttttct cctcgtctat cttcaactga
      661 taaatctttt gcagttgttc cagccacttc ttgagttcct gcgctgcggt ttcgatggga
      721 ttgtcagcca tagggagtga gaggaaaaat tttggactta ggggttgcgc gtgttgccag
      781 ggcgtgcggc gatgatttca ttcggcaggg cgcgattcgg gaaaacggcg ctgcggtaag
      841 tgtgagcggg ccggtaggct ggcttgggcg gcgtggccag cacataatag tgcgtccagc
      901 gcatcccggc gtcgcgacgg cccactccct ccgcccagag ccgcgcttcc ttgccggaca
      961 tgggccgggg aaggctgagc acatggatgt tggatttctg ccagccgatc tcctcgccgt
     1021 cctcattttt ggcgtcccgg taaacccaga attccaccag cgtgccgggc ggcagaagct
     1081 gcgcgtctcc aagcggcatc ggcccgcttt ggcaggaggg cagcaggctg aacgcgatga
     1141 gggccagcgc ctgccagcag cggcggagtg aggaggaggc tacgggggtg tggtgcatga
     1201 ctggctgggg tggattagcc gctcaggtct cgtgtgtgac aggcggtttc ggcggttcgg
     1261 gcggcggctc gtggatgaat gacgcgccgg gcaccggctc gtcctcctct ttgccggcgg
     1321 gcgggggcgc ggacgatgcc tgtttggcgc gcgcgcccaa aaagtctccg acggcctgct
     1381 tgagttcctg catttttctc tgctgctcca accgccgctg ctccatgagc atgcgcccgc
     1441 gcaggaggtg ctcctccccc cagcggtcca cggcgtagat gaccaggatg ccgcccagaa
     1501 tgaggcaggc gacggggatt ttccccatcg ccagggcgct tacaaagagc agcgtgcccg
     1561 tgccgtagcg agtgaggaag cgccggtgcc gcgcccaaaa ttcacggatg gatgaccaga
     1621 tgcccatttt ccgcgacgag tgtgcctgat gtcggtctgc cggccatgag cgcctcgcgt
     1681 acggcgtggc ggcagttggc ctcggcggtg gccctcatgc ggcgcacgcc gaggcgcggc
     1741 tccacgccct gggccacgag caggtccagg acgccggggc cggccttgag gtcgtagccc
     1801 ttgccgcgca ggagttttaa ttccttgttc agcatgatct gggcgatctg cttttgcgcg
     1861 gcgtagccaa gtttttgaaa gacgcagcgc acattgaaac gggcgagcac ctcggggcgc
     1921 agctcggcgg cggcgaggtc ctcgatgaag cgctcgacgg tggccatggg gctgtttttc
     1981 agctccatca aggcgtctgc gccgatgttg gaggtggcca cgatgtagaa ctcgcaaagg
     2041 ttcagcgtct cgccactggc cacggtgatg cgcccggcgt cgagcacctg caggaagatg
     2101 tcgagcacac gcggatgggc tttttcgatc tcgtcaaaga ggatgactcc cctgccctcg
     2161 gcgcggtcgt aaaagcggcc catgttgccg cggctggact cgtccttgcc gagcaggagg
     2221 ccgagggact cctggttttg atactcggac atgtcgagcc ggatgcagtg cttctgcacg
     2281 tctttgaaaa tgtattcggt gatcagcagg gtgatctcgg ttttgccgac accggtgggg
     2341 ccgaggaaga gaaaggtgcc cttcggcctg cccgggtcgc tgaggccgag ttcgccgttt
     2401 tggatgatgg gcacaactct ggggacgacg tggtcctggc cgatgatgcg ctccttgaga
     2461 aaggcgggca ggccctggat gtgctggagg tctggcatgt ggaggccggg acggttcaag
     2521 gctttctggc gccggactgc tgggaggtca tgtcataaag gcgctgctgc tcctcaagcg
     2581 ctttgatgcg ctcctgggcg ttcttgagct gctcctcctt ttcatcgcgg tcggcgcgga
     2641 tttcggccag gtattgcttg atctggacgg cctcctcctt gccggcgtcg aagtcggcgc
     2701 ttttcttttc cagagtgcgg cgcaactcat cgaggcgggc catggatttc tgcaaattga
     2761 gcgccatggt ggcgagcctc tcgcgctcct gggcgatgag aatgtccttg ggcagcgaat
     2821 cacgcgcggg gtcggtgatg ccgaggatga cgccgccgga gtccatgggc gtggcgggca
     2881 ggcgggtgtc ccagcgcgcg tcctgctcga cgcggtaaac cgtgtgcgcc tcgtgcatct
     2941 cacgccggtc ccgcgagggc aggcggccga ggtggtaggc gtggacttcc tcgccgaggc
     3001 gcacgcggga aacatcgcgc ggatgcaggg agcgtccgcg cggccagccc tggacgactt
     3061 ctttggaata ggtggtgatg ccggcgagct cctgctcgtc ctcgggacgg acgaggggat
     3121 cgtcggaaag gtccgtcttt ttcgagcggc aggaggcgcc ggtcaaaacg aggagcgcga
     3181 ggagaagggc gtgatggagt ttggggatca tggctcgctg cgggtggagg gggtggatgg
     3241 tgctgaatcg ccggcgacaa tgccgcgcac ccggctgacg ggagcgagcg cctgctgggc
     3301 ggcacggacc tgtcctgcct gcgcctcggc agcggcggca ttctgcttga gggcggcctg
     3361 ctcggcttgc gcgcgggcgt aagtctcctc ggcgagcttc cagcttgaga caggctgctc
     3421 tccctggacg agacccgcga cgctggcgag gccgggttca aagacatcga gagtgaaaat
     3481 ataaaactgc gtgccggccg ggacgcggac aaagtcaagg tcctgcgcca gcctgtctgt
     3541 gagcatgcgc gagtaggcgg aagcggcctg ggagccgccg ccgagggccg cgttgcgatt
     3601 ggtgttttgc ggggcgagtc cgtagatgct ctggaactgg tcctgctggt tgttcatgat
     3661 gccttgaagc gcctcggcca cgagaatctt gagttcggcg taggcgtcgg ccttgacgat
     3721 ctgcccgcga atgccggcgg agccgtccgt gatgccatag gtgccgtccg gcagccgctc
     3781 gtggtcgagg gcgactccgt taatgcggta ctcgcgcccg tccgcccaga tgaaagtgta
     3841 agcgcccttg acttcgatgc ggtcgcggct gcgtccctgg gccgcctggg ccaggacctg
     3901 ggtgcccgcc ggcacgataa gccggccttt gaaatacaca tcctcggtga ccagggcgag
     3961 caccggtgtc tcaagcgagg aggagtccac cgtgatgacg agcgccgcct tgatcagcag
     4021 gccgcgcggc gcgaaaaagc tcggcggctg gggcacatgc ggctcgacga tgtcattttt
     4081 gacatggacg agccttggca gcggcggcgg cccgctcttt ttctgcgggc ctgagtccga
     4141 ggaagtctcg atgagggctg gcggcggctt gggcttctcc ttgggcagca tgagcctgga
     4201 cacgtccgag ctgacctcgg cggtgcgctg ctggccggcc tcgacttttt tctcctccac
     4261 cgccatctgg cccactccct tggtgcgcgg ggtctcgttc tcgggcggca cattcaccgg
     4321 cggcggcggg cgtgtgctga ggtagtaggc ggcgccgccg atggcgacga ccgcgaacag
     4381 aagcgccatc tggacgccgg gtttctggag gtgcttcatc atggcggcgg ctcactttcc
     4441 atcgtcaagg gttgcagtgg cgccgccaaa gagggcggcg gaggcgcggg tgcgtcccgc
     4501 ctggggcagg tctatgcgga aatcattgtc tatggaaaga tgctcgcgcc ctccgccagg
     4561 tccgccctgg agcacgacat ccatggcgat tttttcattc ggcatcacga tccccgaaca
     4621 gtccacaagc tgggcggcgt aggcgcgctc gtcggcgcgc acgcggatgt ccacgggatc
     4681 gaactcgtaa gccgcggcgc cggtgttgtt cagccagcag cggaagacga gcacatcctt
     4741 ctccggccag cgctggattt cataaatggt gcaggtgacg ccgtccttgg cggtgctcaa
     4801 ctccaggccg ttgcgctcct gccagcccat gtagagggct ggattgacac tttgaaggaa
     4861 cttgcgctga cgcgccctgc ccagcatgcc gacaagctcg tcggaattgt aggtgatgcg
     4921 tttttgggcc acctcgaccg gggcgacctc cctggccgtg ttgcgctggc cgggcggcgg
     4981 aatgatgacg gccaagtgag cctcgacatc ggcggcgaag cgcagcaggt ggatgttttt
     5041 tccagcgcgc acggtgacat ggcagggatc accctccttc aaaaggcgca cgaccagggt
     5101 gtcggacatc acaagctcgt agtgaaccac gctcgtgcgg ctttgcgcca tgcccgagac
     5161 ggcggcgctg tcttccacga ggccgaaacc ggtgagcatg ctgaccggct cgggaaaaat
     5221 gacggaggtg gccacatggc tgttgatgtg gatttcatga acggcggatg ccgggttgag
     5281 catgaccatc tcattcccct ccttgacctg ggcgtgaaga agcgcgggga ggagcgtgtt
     5341 catcagaacc gcgaggatgt tcaaacacgg atattggatt ttccttctca tggcatgctg
     5401 agcgggatgc gctcgtagga gtagaggttg aaaagcagcg cggggaagga ctcattgccg
     5461 cgcacatcgg ggttgagcac ccattggaat ttgattttga aggtggagac ttcctcgacc
     5521 gcgccgccga actgcccgcg ccggaagatg cgcccggtgg cctgggtgcc cgccgccgtg
     5581 ccggaagcgc ccaggctctt gacctcctcg atctcatagg tctgccgcac ctgctggcga
     5641 ctgaagtttg aggactcctc ctgaatgagc ttgttgatct ggccgagcgc gtcgcgcgtg
     5701 cagagtttgt tcagccgcct gccgtaagcc agcccctccg gcccgcgtgt gagcatggtg
     5761 tccaccagga tctctgtcag cgccaggtgg atgggggtgg ctttttcgaa gttcagtcct
     5821 ggcgcggaat agaagacccc cgcgccatcc atgatgacga agggcgggcg ctccttcatc
     5881 gtgtcggaaa ccttcaccac ataccaggag cacgctgccg ctgtcaggca ggcgatcagg
     5941 aaccaaaaca cggccacacg atccttcgcc acgaaggcgt ccaccgcgcc gcgccgcttt
     6001 ttcggagtta tgtctggctt ggcctggctc atgattccga aacaagggtg agtgtgtagg
     6061 ccgcctcgcg gcaggtgtgc gggtagagct tgttgtcgcg cagattgatg ttcggctccc
     6121 agacgaggcg cgcaccgaga ttgaagacct ggttgacgac cttctgctgc accggatcca
     6181 cgccgacgcg gatgacctgg ccggttgcca ttgctatggc gcccccgtcc tcgccgagaa
     6241 aaacgcgcac cgagcccacc tccgccattt ggtgatactg ccgggagcgg aactcctctc
     6301 gattctccgc caaatccttc atcaaccact ccagggcggg tccgccgaaa attttggcca
     6361 cccgcttctg cgtgaccggg ccgttcggag cgcggttgag catggtttcc agtgccagac
     6421 gcgtctgggt gtcgaacaag tccgaagccg tctccggctg cagatcgcgg tccaggtaca
     6481 tgacatcggg ccggttgacc atgacgtaaa gcatccggct tttgccatcc ccggcgatgt
     6541 cgttcacata ccagcccgtg cccgcgagga tggccacgca gacgaagaac cagaaaaaag
     6601 ccagcttttc cttggagatg aagagatcgg agacgccttt catgtgtcag tggatcaaag
     6661 aaaatcagtt gcccgcgctc gtcacgccct tgctcagggt gcccgccgcg ccgcccattg
     6721 tgcccgcgcc cccagccatc atgccgacgc cgacagccgc gccggcgcct ccagttcccg
     6781 cggtgatggc cgcgcc
//
LOCUS       DAOMYJ010000260         6701 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Prosthecobacter sp. isolate AMR_MDS_5629
            NODE_3877_length_6701_cov_15.142040, whole genome shotgun sequence.
ACCESSION   DAOMYJ010000260 DAOMYJ010000000
VERSION     DAOMYJ010000260.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867850
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prosthecobacter sp. (wastewater metagenome)
  ORGANISM  Prosthecobacter sp.
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Verrucomicrobiaceae; Prosthecobacter.
REFERENCE   1  (bases 1 to 6701)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6701)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.30
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:34:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,742
            CDSs (total)                      :: 4,684
            Genes (coding)                    :: 4,676
            CDSs (with protein)               :: 4,676
            Genes (RNA)                       :: 58
            tRNAs                             :: 55
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6701
                     /organism="Prosthecobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3877_length_6701_cov_15.142040"
                     /isolate="AMR_MDS_5629"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1965333"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            139..642
                     /locus_tag="PLP58_19445"
     CDS             139..642
                     /locus_tag="PLP58_19445"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014566.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HRJ10765.1"
                     /translation="MSAALGAGIVEEFFFRALLLGLLMRTLRAGPAVFWTTFIYALVH
                     FLKPPARWKIADESVTWTSGFEVLAQIARGFGDVDFLLAEFATLFAVGWVLARARLRT
                     GALWAGIGLHGGWVFGLKYFSALTRHPGGWLPWIGENLKIGLLPLATVLLTGWLALRL
                     LPRQADK"
     gene            688..769
                     /locus_tag="PLP58_19450"
     tRNA            688..769
                     /locus_tag="PLP58_19450"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:722..724,aa:Leu,seq:tag)
     gene            complement(803..1435)
                     /gene="bioD"
                     /locus_tag="PLP58_19455"
     CDS             complement(803..1435)
                     /gene="bioD"
                     /locus_tag="PLP58_19455"
                     /EC_number="6.3.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009963299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004141 - dethiobiotin synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dethiobiotin synthase"
                     /protein_id="HRJ10766.1"
                     /translation="MHYFITGTDTDAGKTYVTCLLIEALRRAGHDAVGYKPFACGDRA
                     DAHALREAGGHALTLEETNPVFFKAPASPCAAAMLENKTVDLEAARQGFDALAARHAS
                     VLVEGAGGWEVPLADGMTMAGFAASLGLPVILVVDNRLGCLNHTLLTLRAIRASGLEC
                     AGIVLNHTRDERDLASISNRAVLERFAEAPVLLDIMHGETRVDWPEALRP"
     gene            complement(1442..3103)
                     /gene="recN"
                     /locus_tag="PLP58_19460"
     CDS             complement(1442..3103)
                     /gene="recN"
                     /locus_tag="PLP58_19460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009965626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HRJ10767.1"
                     /translation="MLSFLKIRHLALVEDVTWELRAGLTGVTGETGAGKSIIVGALKL
                     ILGERADRSLIRTGQDTCTVEACFHLPDTRAVDAVLEEAGLEPCQDGELLIKRSISAG
                     GANKQFVNCSPVTIQVLKSLGEMLVDLHGPHDHQSLNSGARQLEMLDKHAGSEDALAA
                     YQEAWRQWRAALGELDELENSERAGSQQLDMLRFQVQEIAAAALQPGEEEEIAARHRV
                     AANGARLAELCGIIISRLGGGEGGVLDALREIARAIHEMEKIDPHCARLFEGYQSAQI
                     ELAELENNAGDYLEGLEPDPEVLARLEQRIHVIQTLKRKYGPTVAAILDFQREAETKL
                     NKIENRAGEIGRLQQLVKDRRAEVDRLAAQIGKKRAASAPKLAAEVAAHLADLGFKRS
                     VFEARLTPLDEPGALGREEVDFLFAPNPGEPLKPLRLTASSGEMSRVLLAVKSALARQ
                     DAVPLLVFDEIDANVGGSIAGAVGRKMASLGGTHQVIAITHLPQVASLAASHFVVTKD
                     IEDDRTRSSIREVSGEERVEELARMLGGKAESAREHARNLLAGRA"
     gene            complement(3232..3957)
                     /locus_tag="PLP58_19465"
     CDS             complement(3232..3957)
                     /locus_tag="PLP58_19465"
                     /inference="COORDINATES: protein motif:HMM:NF015297.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase/citrate lyase family protein"
                     /protein_id="HRJ10768.1"
                     /translation="MNTAPLQLGTWISTGSAAVTELAALCGLDWVLLDLEHGCESEAA
                     IPGQLRSLRGGKTRGIVRVGAPHADLIGRVLDWGADGVMVPHVESAAAAEAIVRAAHH
                     APRGQRGFARTVRACDYGLRPAEEKARPLLMAQIETIAGVGHCADIAAVEGIDVLFVG
                     PADLQHDLRSRPERAPGDYARCLEIVNEAARAAGRQTGILIREAGGVERHRELGFTHI
                     AVDSDLSILRKAWRRTAEAAEGG"
     gene            complement(3954..5012)
                     /locus_tag="PLP58_19470"
     CDS             complement(3954..5012)
                     /locus_tag="PLP58_19470"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013775.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside phosphotransferase family
                     protein"
                     /protein_id="HRJ10769.1"
                     /translation="MSRRDIYYWKCDRPAAFHGTRGHGAAAETEPALRAALQEHFHTR
                     DVTLRPGAGQGNHLTWLADVDGRALFLRVEDGPEGDTHLAAESAVLDAVRACGVPTPR
                     VFGCDASRLRAPFAWQALERVDAPDLNQWFKDGALDTHAAAFEIGAAVARWQSAPVTG
                     FGPLDAGLRGCHAAYPAYFHLRLERHLAFLVERRFLSRARADEFAAAIAEHGALLDLE
                     KGCLVHKDLALWNILGTTRGIRAFIDFDDAISGDAMDDLSLLGCFHDAAFVARAIEGY
                     QSLRALPAESARRFWLHLLRNMIVKAVIRVGAGYFERDAGFFLIQNGGGSALRERTLE
                     RLALALRGLREDAPLSLL"
     gene            complement(5009..5962)
                     /locus_tag="PLP58_19475"
     CDS             complement(5009..5962)
                     /locus_tag="PLP58_19475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009963460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bile acid:sodium symporter family protein"
                     /protein_id="HRJ10770.1"
                     /translation="MMHRFLSWFTRLYPVWLVGTAVFGFWRPERLDWFAGQWIVWALS
                     VSMLGMGLTLSPADFRGLLRMPGAAALGFACQYTLMPLTGWVVAWALGLEPGFAVGLI
                     LVCCCPGGLASNMITFLAGANLALSVVLTMTSTLLAFAATPLWTWALAGQYVPVDAWG
                     LCKSTLQAVIVPVLLGVFCNWRFPRAVTRVAPYGPAVSVVALCLITGGIVAVRAPLIA
                     QYFGTLLAAALAIHVLGFGLGYAVGKALRYDEIVARTVSIEVGMQNGGMASMLASLHF
                     AAQPLAAVPAVFSGVMQNILGGLLASWWKARPPVVMAKNDS"
BASE COUNT         1062 a         2314 c         2335 g          990 t
ORIGIN      
        1 ggcgtccaac cttccaggaa gggtcggcgc cagtgatcgg ttacgctggg gctgctgctg
       61 gcgctgggcg cgggctttgt gctggcgggc gcgcacacgc tgcgcgagga gccgaaatgg
      121 ccggcctttg gcgaacccat gagcgccgcg ctgggcgcgg gcatcgtgga ggagttcttc
      181 ttccgcgcgc tgctgctggg cctgctcatg cgcacgctgc gcgcggggcc ggcggtgttt
      241 tggaccacgt tcatttacgc gctcgtgcat ttcctcaaac cgcccgcgcg gtggaagatc
      301 gccgatgaat ccgtgacgtg gacgagcggc ttcgaggtgc tggcgcaaat cgcgcgcggc
      361 tttggcgacg tggacttcct cctggcggag ttcgccacgc ttttcgccgt gggctgggtg
      421 ctggcgcgcg cgcggctgcg gacgggggcg ttgtgggcgg gcatcggcct gcatggcggc
      481 tgggtttttg gattgaaata tttcagcgcg ctcacccgcc accccggcgg ctggctgccc
      541 tggatcgggg agaacctgaa aatcggcctg ctgccgctgg cgacggtgct gctcaccggc
      601 tggctggcgc tgcgcctgct gccgcgccag gcggacaaat gatttgcagc gcggcggaag
      661 cggacaaact atcccgccgc gctccctgcc ggagtggcgg aattggtaga cgcgccagac
      721 ttaggatctg gtttggcaac aagtgcaggt tcgagtcctg tctccggcac tttgaagcac
      781 gacttcgcag cccggaacag gatcacggcc gcagcgcctc aggccagtcc acccgcgtct
      841 ctccgtgcat gatgtcgagc agcacggggg cttcggcgaa gcgttcgagc acggcgcggt
      901 tggagatgct ggccaggtcg cgctcgtcgc gtgtgtggtt gagcacgatg ccggcgcact
      961 ccaggccgct ggcgcggatg gcgcgcaggg tgagaagcgt gtggttcagg cagccgaggc
     1021 ggttgtccac gacgaggatg accggcaggc caagactggc ggcaaagccg gccatcgtca
     1081 tgccatcggc cagcggcacc tcccagccgc ccgcgccttc cacgagcacg ctggcatggc
     1141 gggcggcgag cgcgtcaaag ccctggcgcg ccgcttcgag gtccaccgtt ttgttctcca
     1201 gcatggcggc ggcgcagggg ctggcggggg ctttgaaaaa gacggggttg gtctcctcca
     1261 gggtcagcgc atggccgccg gcctcgcgca gggcgtgggc gtccgcgcgg tcgccgcagg
     1321 cgaagggttt gtagcccacg gcgtcatgcc cggcgcggcg cagggcctca atgagcaggc
     1381 aggtgacgta ggtcttgccc gcgtccgtgt ccgtgccggt gatgaagtaa tgcatggcgg
     1441 ttcaggcgcg gccggccagg aggttgcgcg cgtgctcgcg ggcggactcg gccttgccgc
     1501 cgagcatcct ggccagctcc tccacgcgct cctccccgga gacctcccgg atgctggagc
     1561 gtgtgcggtc gtcctcgatg tccttggtga cgacgaaatg gctcgccgcc agtgacgcca
     1621 cctgggggag gtgggtgatg gcgatgacct ggtgcgtgcc gccgagggag gccatcttgc
     1681 gcccgaccgc cccggcgatg ctgccgccga cattggcgtc aatctcatcg aagacgagca
     1741 gcggcacggc gtcctgccgg gccagggcgc tcttcaccgc gagcagaacg cgggacatct
     1801 cgccgctgct ggcggtgagg cgcaggggct tcaatggctc gccgggattc ggggcgaaga
     1861 ggaaatccac ctcctcgcgc ccgagcgcgc cgggttcatc gagaggcgtc agcctggcct
     1921 cgaagacgct gcgcttgaag ccgaggtcgg ccagatgcgc ggcgacttcg gcggcgagct
     1981 tgggggcgga ggcggcgcgt tttttgccga tctgcgcggc cagcctgtcc acctcggcgc
     2041 gccggtcttt gacgagctgc tggagcctcc cgatctctcc ggcgcggttt tctatcttgt
     2101 tgagcttcgt ctcggcctcg cgctggaagt cgaggatggc ggcgaccgtg gggccgtatt
     2161 tgcgcttgag ggtctggatg acgtggatgc gctgttcgag gcgggcgaga acttccgggt
     2221 cgggctccag cccctccaag taatcccccg cgttgttttc caactcagcc agctcgatct
     2281 gcgcggactg gtagccttca aacagccggg cgcagtgggg gtcaattttc tccatttcat
     2341 ggatggcgcg ggcgatctcg cgaagggcgt ccagcacacc gccctcgccc ccgcccaagc
     2401 ggctgatgat gatgccgcag agttcggcga ggcgcgcgcc attggccgcc acgcgatgcc
     2461 gcgcggcgat ctcctcctcc tctcccggct ggagcgcggc ggcggcgatc tcctggacct
     2521 ggaagcgcag catgtcgagc tgctggctgc cggcgcgctc gctgttttcc agctcgtcca
     2581 gctcgcccag agcggcgcgc cactgccgcc acgcctcctg gtaggcggcg agcgcgtcct
     2641 cgctgccggc gtgcttgtcg agcatctcga gctggcgcgc gccggagttc agggactggt
     2701 ggtcatgcgg gccgtgcagg tccacgagca tctcgcccag gcttttgagc acctggatgg
     2761 tcacgggcga gcagttgacg aactgcttgt tcgccccgcc ggcgctgatg ctgcgcttga
     2821 tgagcagctc cccgtcctgg cacggctcca gccccgcctc ctccagcacg gcgtccaccg
     2881 cgcgcgtgtc cggcaggtga aaacacgcct cgaccgtgca ggtgtcctgc ccggtgcgga
     2941 tgagggagcg gtccgcgcgc tcgcccagga tgagcttcaa cgcgccgacg atgatggact
     3001 tgcccgcccc tgtctcgccc gtgacgccgg tcagcccggc ccgcagctcc caggtgacat
     3061 cctcgacgag ggccagatgg cggattttga gaaaggacag cattgcggcg gataggtgcg
     3121 cgaagatggg gaaggctggc ggcaatgcaa ccggctcgtc ttgtggaatg cggcggccgc
     3181 ctcccgccgt ttcgattttc gaaagcgccg tcacacctcc gatcccgccg ctcatccgcc
     3241 ctccgcagcc tccgccgtcc gccgccaggc cttgcgcagg atggagaggt ccgagtccac
     3301 cgcgatgtgg gtgaagccca gctcccggtg gcgctccacc ccgccggctt cgcggatgag
     3361 gatgccggtt tgcctgcccg cggcgcgggc ggcctcgttg acgatctcca ggcagcgggc
     3421 gtaatccccc ggcgcgcgct ccggccggct gcgcaggtcg tgctggaggt cggcggggcc
     3481 gacaaagagc acgtcaatgc cctcgacggc ggcgatgtcc gcgcaatgcc cgacgcccgc
     3541 gatcgtctcg atctgcgcca tgagcagggg ccgggccttt tcctcggcgg ggcgcaagcc
     3601 gtagtcgcag gcgcgcacgg tgcgggcgaa gccgcgctgc ccgcgcggcg cgtgatgagc
     3661 cgcccgcacg atggcttcgg cggcggcggc ggactcgaca tgcggcacca tgacgccgtc
     3721 cgcgccccag tcgagcacgc gcccgatgag gtcggcatga ggcgcgccga cacggacgat
     3781 gccgcgcgtt ttgccgccgc gcagggagcg gagctggccg gggatggccg cctccgactc
     3841 gcagccatgc tccaggtcca gcagcaccca gtccagcccg cagagggcgg cgagttcggt
     3901 gacggcggcg gagccggtgg aaatccaggt gccgagttgc agaggcgcgg tgttcataaa
     3961 agggagaggg gagcatcctc gcgcagaccg cgcaacgcca gggcgagccg ctccagcgtg
     4021 cgctcccgca aggcgctgcc gccgccgttt tggatgagaa aaaagccggc gtcacgctca
     4081 aagtatcccg cgccgacacg gatgacggct ttgacgatca tgttgcgcag caggtgcagc
     4141 cagaagcggc gcgcggactc cgcgggcagc gcgcgcaggc tttgataccc ctcgatggcg
     4201 cgggccacaa aagcggcgtc gtggaagcag ccgagcaggg agagatcgtc catggcatcg
     4261 ccgctgatgg cgtcgtcgaa gtcaatgaag gcgcggatcc cgcgggtggt gccgaggatg
     4321 ttccaaagcg cgaggtcctt gtggacgagg cagccttttt ccaggtccag caaagcgccg
     4381 tgctccgcga tggcggcggc gaactcgtcc gcgcgcgccc ggctgagaaa ccggcgctcc
     4441 acgagaaaag ccaggtgccg ctccaggcgc aggtggaagt aggccgggta agccgcgtgg
     4501 cagccgcgca gcccggcatc cagcggacca aagccggtga cgggcgccga ctgccagcgc
     4561 gccacggcgg cgccgatctc aaaagccgcg gcatgtgtgt cgagcgcgcc gtctttgaac
     4621 cattggttca agtccggcgc gtccacccgc tccagcgcct gccaggcgaa gggcgcgcgc
     4681 aagcgcgagg catcgcagcc gaaaacgcgc ggcgtgggca cgccgcacgc gcgcacggca
     4741 tccagcacgg cggattcggc tgccaggtgc gtgtcgccct ccggcccgtc ctccacgcgg
     4801 agaaaaagcg cgcgcccgtc cacatccgcc agccaggtga ggtggttccc ctgccccgcg
     4861 ccgggccgca gcgtcacatc ccgcgtgtgg aagtgctcct gcaaagccgc gcgcagcgcg
     4921 ggctcggtct ccgccgccgc gccatgcccg cgcgtgccgt gaaaggcggc ggggcggtcg
     4981 cacttccagt aataaatgtc gcgccggctc atgagtcgtt ctttgccatg accaccggcg
     5041 ggcgtgcctt ccaccagctc gcgagcaggc cgccgaggat gttttgcatc accccgctga
     5101 agaccgcggg cacggcggcc agcggctgcg cggcgaagtg caggctggcc agcatcgaag
     5161 ccatgccgcc gttttgcatg cccacctcga tgctcaccgt gcgcgccacg atctcgtcat
     5221 aacgcagcgc cttgcccacc gcgtagccga ggccgaagcc gagcacatgg atggccagcg
     5281 cggccgcgag cagggtgccg aaatactgcg cgatcaaagg cgcccgcacc gccacgatgc
     5341 cgcccgtgat cagacacagc gccaccaccg acaccgccgg gccgtagggc gccacgcggg
     5401 tcaccgcgcg cgggaagcgc cagttgcaga acactcccag cagcaccggc acgatcaccg
     5461 cctggagcgt ggacttgcac aggccccagg catccaccgg cacatactgg cccgccagcg
     5521 cccacgtcca cagcggcgtg gccgcgaagg ccagcagcgt cgaggtcatc gtcagcacca
     5581 ccgacagcgc caggttcgcc ccggccagga aagtaatcat gttcgaggcc agcccgcccg
     5641 ggcagcagca caccaggatc agccccactg cgaagcccgg ctccagcccc agcgcccatg
     5701 ccaccaccca gccggtcagc ggcatcagcg tgtactggca ggcaaacccc agcgccgccg
     5761 cgcccggcat ccgcagcagc ccgcggaagt ccgccggact cagcgtcagc cccatgccca
     5821 gcatcgagac gctcagcgcc cacacgatcc actgcccggc gaaccagtcc agccgctcag
     5881 gccgccaaaa cccaaagacg gccgtgccga ccagccagac ggggtaaaga cgggtgaacc
     5941 aggagagaaa gcggtgcatc attcaggtgt agcggacagc gcggacaaaa cgcaagcccc
     6001 ccttttctcg gagacccggc ccgccgcgcc accccgcttc tacgcatcat ccccgcgact
     6061 tccacgaccg gaaacccgcg aaaccctggt atgctaccac agcccggccc gcccccgtgc
     6121 gcgtcaggca gggcgcgttg cccccggcgc gccttggccg ccatgccaca cgtccacggg
     6181 tcctgggaaa gcccgtgttt ggatcaaccc aacagggctt gcccatcctt gccaggcaag
     6241 gattgaggca aagccgctac cctggaataa ggatgccaca cccgcgcccc cggagcagcc
     6301 tcagggctgg cggcaagact cgcgtccggc aggaaggcag actccgacgt gggaaaaaac
     6361 agggcgcgtc gccggaatga tgagcgtagg acgactgtgg ctttagcccg gtcgtctggt
     6421 gggcgcacgc tgcaacgacg cctctccaaa gccgggcggg ctgcgccaca cccggcctgc
     6481 atccttctgg cgacgcaccc aaaaacatcc ccgtcggccc ggtggagcct cgggaccggc
     6541 ggcaagacag atttcggcat ggggaacggc atccctgccg gcccggtgga gcctcaggac
     6601 cggcggcaag actcgcgtcc ggcaggaagg cggactccgg gacggaagaa aacaagcccc
     6661 gcgcgcccag ggcagcctcg ggaccggcgg gaagactcgt c
//
LOCUS       DAOMYJ010000261         6642 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Prosthecobacter sp. isolate AMR_MDS_5629
            NODE_3927_length_6642_cov_19.761348, whole genome shotgun sequence.
ACCESSION   DAOMYJ010000261 DAOMYJ010000000
VERSION     DAOMYJ010000261.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867850
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prosthecobacter sp. (wastewater metagenome)
  ORGANISM  Prosthecobacter sp.
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Verrucomicrobiaceae; Prosthecobacter.
REFERENCE   1  (bases 1 to 6642)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6642)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.30
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:34:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,742
            CDSs (total)                      :: 4,684
            Genes (coding)                    :: 4,676
            CDSs (with protein)               :: 4,676
            Genes (RNA)                       :: 58
            tRNAs                             :: 55
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6642
                     /organism="Prosthecobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3927_length_6642_cov_19.761348"
                     /isolate="AMR_MDS_5629"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1965333"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..667
                     /locus_tag="PLP58_19480"
     CDS             <1..667
                     /locus_tag="PLP58_19480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ10771.1"
                     /translation="VELVPDVVANARDAARRAPALVMAGLCLWAGLGAGIMYFKNTAR
                     VTQEKLAGMQQESAALTGLANQIRALDGQLAQTLALTDPLIKAVGDRSYWVRLLTEMN
                     SRFDNDLIWLTTVELLKDGKSITPALFGQEQSSSSSKSGAAGQPVYELQLQGLYRKNE
                     EGEQIVYRYAGALAKSGWFAADKFEEKRAEYVSAESGVEEDRYAYKFNIKLPLQQPMQ
                     FQN"
     gene            691..1680
                     /locus_tag="PLP58_19485"
     CDS             691..1680
                     /locus_tag="PLP58_19485"
                     /inference="COORDINATES: protein motif:HMM:NF038287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Amuc_1100 family pilus-like protein"
                     /protein_id="HRJ10772.1"
                     /translation="MPAAIMGLSAAGAAGLGFMMFQSWQEHSTALEQFDNLNRGIQGL
                     NSAVLAPTPQNLELKKGLVGELTTEAGKLNTVLTRLQGDVKPEAISDTGFQAKLKTRI
                     AEVRNLAAGRMALPAEFNLAFDRYNSELPKSNDEATELSGYLDAVESIVKSMVQSGVA
                     QLDMISRSDLPSEKSSAPAPKSKGGQPAPAAPQLVKRQTVTAVITTDQAALQLLLSKL
                     ASPSEMPYFTVVRLVRIENERKEGPLRADVGLHTITPPEGTEAADNAALIVVAPPVPA
                     PGGANPPEKDDTIAAVQPMNQDAVDVLGKEALKVYLEIDIISFQTTASAAPGR"
     gene            1725..2687
                     /locus_tag="PLP58_19490"
     CDS             1725..2687
                     /locus_tag="PLP58_19490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ10773.1"
                     /translation="MQKKNGNYEKVLLGVCALVAVGIAGFLVWQSQQFEERLTRLQAS
                     SKNDPGQPPTEIVKATIQRLVEKVSWVSPVIKGKPVPLNKSVRLVLKGDQLIDLMTEE
                     VQLRPPMPNSFIVANDLPNYLFANFGDLDADEDGFSNLEEFNAKTNPKDSGSHPPVTD
                     KLFLARRITHDYIIRLNSTSDPFQVQRILPAPDRPVSKFVSVGADFGFEKGSERFRTI
                     KFEKKTIPDPSVGEKDVSELTVLDKATNKEFVVAKGTDTNLAEYEAEFEFLLGKRQTR
                     VAKKGETFQIPGIGQTFRLLEVEEDHAVIQPVEEGSKPITIRKA"
     gene            2786..5281
                     /locus_tag="PLP58_19495"
     CDS             2786..5281
                     /locus_tag="PLP58_19495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009959352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II and III secretion system protein"
                     /protein_id="HRJ10774.1"
                     /translation="MMHPSRIKKPQIALLVALAAACSAVTGQAGEGGLGVPGIAEREI
                     ARRAARIEDARQAMAKGDELFAQGKYEDALAQYRSAKDTLDQLPNAPFIQDWRDLANL
                     KFADCAVVVAREKAKVGDYAASRKLLEEAQLAVPGHKAAALFAKQMNDPDRWPPALTP
                     QHVENVSKVTQQLTLANSSMEIGNYDDALDQYEDVIRIDPYNVAARRGMEAVEQKRAQ
                     YFDASRDHQRSRMLNMVNEAWEHKPPVRGLRIDSLQTGGEEPTVYLTRKMQQLIFPSV
                     QFAGASIEEAVEFLRVKSKDIDVAETDPTKKGVNIILKAGDAPITASISLDLRDVPMV
                     EALRYVTELAGMKYKVEPYAVLIVPVSDISDNQYTRIYKVRPDFLSLGGTDAAAAPAA
                     SADPFAAGNAGAAPASGLLQRKTAIEILKAQGIPFPEGSSAVFNPVTSQLIVKNTQPN
                     LDLVEAFVEASKTDAPKQIYITSKFVEVSQKNTDELGFDWLLGGWGGEVLAGGGTDGN
                     SGLNTNTMDYPVTFNPLTTFDPVTGAVVSRAISPVSRGLRTGSRAITGNAIDATIAQG
                     IGGQIGATEAPGVFSLAGVLTDPQFGVVIRALAQRKGVDLMSAPSVTTKGGQRATIEV
                     VREFIYPTEFDPPQVPTNVGGGGALGALGGGTTTIPVTPTTPTAFEMRPVGVRMEVDP
                     TVGADGYTIDLNLLPEVTEFDGFINYGSPILSNGVVLTENIINQPVFSTRKVTTAVTV
                     WDGQTVVLGGLIREDVQDVEDKIPLLGDLPWVGRMFKSQAEDHFKRNLMIFVTAKLID
                     PAGQPINTNLGGGGAEGIGSGPSTDLLPPVTGL"
     gene            5355..5966
                     /gene="coaE"
                     /locus_tag="PLP58_19500"
     CDS             5355..5966
                     /gene="coaE"
                     /locus_tag="PLP58_19500"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: protein motif:HMM:TIGR00152.1"
                     /note="Dephospho-CoA kinase (CoaE) performs the final step
                     in coenzyme A biosynthesis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004140 - dephospho-CoA kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HRJ10775.1"
                     /translation="MKTWVITGGMGCGKTFFLDVLFRSVEEKVEVFSADAEVRRLMSL
                     PETLQSILEVFGPEAVIQEAGADKVNRDWLRGLVFSDPSHRSSLEALLHPGVLSSLEK
                     RRREARVAGVNLFLAEVPLHYEIQSTVSADLVIVVASSRAVQVRRLMETRGLDQSIIE
                     KMLSAQLPIEAKVEKADVVIWNDGRPAALEAQALTLARQIRHE"
     gene            5963..>6642
                     /locus_tag="PLP58_19505"
     CDS             5963..>6642
                     /locus_tag="PLP58_19505"
                     /inference="COORDINATES: protein motif:HMM:NF019138.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ10776.1"
                     /translation="MNPIETTPPPLVPSEPAGTASEAAPRTLAPAVDGESAPEATEAS
                     EQPQPSETAPAEPGGGLSPVEKQSPAAEPPFPVEISLNDVQEMPLSALQELASSVGFR
                     LNASRTKHQLIYDLLSWMADHRTRVKVDGILEIGADNYGLIRYPKYSFAPLPEDVFIP
                     LFLVRKFQLRPGNRITGFARAPKDRDKYLAIDRILEIDGIPVETWEPPVHFDKLTAMF
                     PNQRIILET"
BASE COUNT         1431 a         2071 c         1897 g         1243 t
ORIGIN      
        1 ggtggagctg gtgccggacg tcgtcgccaa cgcgcgtgac gccgcgcgcc gcgcccccgc
       61 gctggtgatg gcggggcttt gcctctgggc cggcctcggc gcgggcatca tgtatttcaa
      121 aaacaccgcc cgcgtgaccc aggagaagct cgccggcatg cagcaggaga gcgccgcatt
      181 gacgggcctg gccaaccaga tccgcgcgct ggacggccag ctcgcgcaga ccctggcctt
      241 gacggacccg ctcatcaaag ccgtgggcga ccgctcctat tgggtgaggc tgctgaccga
      301 gatgaacagc aggttcgaca acgacctcat ctggctcacc acggtcgagc tgctcaagga
      361 cggcaaatcc atcacaccgg cgttgttcgg ccaggagcaa agctcgtcct catcgaagtc
      421 cggggcggca ggccagccgg tgtacgagct gcagctccag ggcctctacc gcaaaaacga
      481 ggagggcgag cagatcgtct atcgctacgc cggcgcgctc gccaagtcgg gctggttcgc
      541 ggcggacaag ttcgaggaga agcgcgccga gtacgtcagc gccgagagcg gggtcgagga
      601 ggaccgctac gcctacaagt tcaacatcaa gctgcccctc cagcagccca tgcaattcca
      661 aaactgatga gctggattca acaaaacaaa gtgcccgcgg ctatcatggg cctgtccgcg
      721 gccggcgccg ccgggcttgg cttcatgatg ttccaatcct ggcaggagca cagcaccgcg
      781 ctggagcagt tcgacaacct caaccgcggc atccagggcc tcaacagcgc cgtgctggcc
      841 cccacgcccc agaacctgga gctgaaaaaa ggcctggtgg gcgagctgac cacggaggct
      901 ggaaagctga acactgtgct cacacgcctc cagggcgatg tgaagccgga ggcgatctcc
      961 gacacgggtt tccaggccaa gctcaaaacc cgcatcgccg aggtgcgcaa cctggccgcc
     1021 gggcgcatgg ccctgcctgc cgagttcaac ctggcctttg accgctacaa ttcagagctg
     1081 cccaaatcca atgatgaggc caccgagctg tccggctatc tcgacgctgt cgagtccatc
     1141 gtgaaaagca tggttcaatc cggcgtggcc cagctcgaca tgatcagccg ttcggacctc
     1201 ccctccgaaa agagttccgc gcccgccccc aaaagcaagg gcggccagcc agcgcccgcc
     1261 gcgccgcagt tggtgaagcg gcagacggtg actgcggtga tcaccacaga tcaagccgcg
     1321 ctgcaactcc tcttgagcaa actcgccagc ccttccgaga tgccctactt caccgtggtc
     1381 cgtctcgtga ggatcgagaa tgaaagaaag gaaggcccgc ttcgcgccga tgtcgggctt
     1441 catacaatca ctccgccgga aggcacggaa gccgccgaca acgctgctct cattgtcgtc
     1501 gccccccccg tgcctgcgcc gggcggcgcc aatccgcccg aaaaggacga caccattgcc
     1561 gctgtgcagc cgatgaacca ggacgcggtg gacgtgctcg gcaaggaggc gctcaaagtc
     1621 tatctggaga tagacatcat cagctttcaa acaaccgcct ccgccgcccc cggccgctga
     1681 cgcggcggcc gcgcgccaga taacagacac atcattttga ttttatgcag aagaagaacg
     1741 gaaattacga gaaagtgctt ctgggtgttt gcgccctcgt cgcggtgggc atcgccgggt
     1801 tcctggtctg gcagagccag cagttcgaag agcggctgac acgtcttcag gcgtcctcca
     1861 aaaacgatcc cggacagccg ccgacggaaa ttgtcaaagc caccatccag cgcctggtgg
     1921 aaaaagtctc ctgggtgtcc ccggtcatca aaggcaagcc cgtgccgctc aacaaatccg
     1981 tccggcttgt gctcaagggt gaccagttga tagacctcat gaccgaggag gtgcagttgc
     2041 gcccgccgat gccgaactcc ttcatcgtcg caaatgatct gccgaactac ttgttcgcca
     2101 attttggcga tctggacgcc gacgaggacg gtttttcgaa tttggaggag ttcaatgcca
     2161 agaccaatcc caaggattcc ggcagccatc cgcctgtcac cgacaagctc tttctcgccc
     2221 gccgcatcac ccacgactac atcatccgcc tgaacagcac ctcggaccct ttccaggtgc
     2281 agagaatcct tcccgcgccg gaccggccgg tcagcaagtt tgtctccgtc ggagcggact
     2341 ttggttttga aaaaggttct gagagattcc ggaccatcaa gtttgagaaa aaaacaatcc
     2401 ctgacccctc tgttggcgag aaggatgtct cagagcttac cgtgctcgac aaggccacca
     2461 ataaagagtt tgtcgtcgca aagggcaccg acaccaacct cgccgaatac gaggccgagt
     2521 ttgaattcct gctcggcaaa cgccagacgc gcgtggccaa gaagggcgag actttccaga
     2581 tacccggaat cggccagacc ttccgtctgc ttgaggtcga ggaggatcac gcggtgattc
     2641 agccggtcga ggaaggcagc aaacccatca caatccgcaa ggcctgaatt ttttccaaaa
     2701 atctgaaaaa aacatctcgc caaaaaggcg aaaaacctga taaagacacg ccgctcaacg
     2761 caaattccgt taaatcgctc cttctatgat gcatccctcc cgaatcaaaa agccacaaat
     2821 cgccctcctg gtcgcgctcg ccgccgcctg ctccgcagtc accggacaag ccggggaagg
     2881 cggacttgga gttccaggaa tcgcagagcg cgagatcgcg cgccgcgcag cccgcatcga
     2941 agatgccaga caggcgatgg caaaaggcga tgaactcttt gcccagggca aatatgagga
     3001 tgccctggcc cagtaccgct ccgcgaagga cacgctggac cagcttccaa acgctccatt
     3061 catccaggat tggcgtgact tggcaaattt gaagttcgcg gactgcgcgg tggttgtcgc
     3121 gcgtgagaag gcaaaagtgg gcgattacgc cgcctcccga aaattgctcg aagaagcgca
     3181 actggcagtc cccggccaca aggccgccgc gctttttgcc aagcagatga atgaccctga
     3241 tcgctggcct cccgcgctga ccccgcagca tgtggagaat gtttccaaag tcacgcagca
     3301 gttgaccctt gccaacagtt cgatggaaat cggcaactat gacgacgccc tcgaccaata
     3361 tgaggacgtg atcagaattg acccttacaa tgtcgcagcc cgcagaggca tggaggctgt
     3421 cgagcaaaag cgcgcgcagt attttgacgc ctcccgcgat catcagcgct cccgcatgct
     3481 gaacatggtt aatgaggcat gggagcacaa gccgccggtt cgcggcctca gaattgacag
     3541 cctgcagacc gggggagagg agcccacggt atatctcaca cgcaaaatgc agcagttgat
     3601 cttcccttct gtccaatttg cgggcgccag cattgaggag gcagtggagt tccttcgagt
     3661 caaaagcaag gatattgatg ttgctgagac tgatccgacc aaaaagggtg tgaacatcat
     3721 cttgaaagcc ggtgacgctc cgatcaccgc ttccatcagc ctggatctgc gtgatgtgcc
     3781 catggttgaa gcgctgcggt atgtcacaga actggccggc atgaaataca aagtggagcc
     3841 ctacgctgtg ctcatcgtgc cagtatccga catcagcgac aatcaataca cccgcatcta
     3901 caaggtgagg ccggacttcc tgagcctcgg aggcaccgat gccgctgcgg ctcccgccgc
     3961 ctccgcagac ccctttgctg ctggtaatgc gggtgctgct ccagcttcgg gactgctgca
     4021 aagaaagacg gcgattgaga tattgaaggc ccagggcatc cctttccctg aaggctcatc
     4081 tgccgtgttc aatccggtga cttcccagtt gatcgtcaaa aacacccagc ccaaccttga
     4141 tcttgtcgag gcgtttgtcg aagcgtccaa aacagacgcg cccaagcaaa tctacatcac
     4201 ctccaaattc gtggaagttt cccagaagaa caccgacgaa cttggatttg actggttgct
     4261 cggcggctgg ggtggtgaag tcctggcggg tggcggtacg gatggcaact ccgggcttaa
     4321 caccaacacc atggattatc cggtgacttt caatccgctc accactttcg accctgtgac
     4381 aggtgccgtt gtcagtcgtg ctatttcccc tgtttcgcgc ggattgcgca caggttccag
     4441 ggccatcaca ggcaacgcga ttgacgccac catcgcgcag ggaatcggag gccagattgg
     4501 tgccaccgaa gctccaggtg tgttcagtct ggcgggtgtc ctcacagatc cgcagttcgg
     4561 tgtcgttatt cgcgcgcttg cccagcgcaa aggtgtggac ttgatgagcg ctccaagcgt
     4621 gaccaccaaa ggcggccagc gcgccacgat cgaagtcgtt cgcgagttca tctatcccac
     4681 cgagttcgac ccgccccagg ttccgaccaa cgttggtggt ggtggtgctc ttggggctct
     4741 tggcggcggc acaaccacta ttccggttac tcctaccacc ccgacggcct ttgaaatgcg
     4801 cccggtaggt gtgcgcatgg aggtggatcc caccgtagga gcggacggat atacaattga
     4861 tctcaatctt ctgcctgaag tgacagaatt cgacggcttc atcaattacg gcagcccgat
     4921 tctgagcaat ggagtagtcc tgaccgaaaa catcatcaac cagcccgtgt tctccacccg
     4981 caaagtcacc acggcggtca ctgtttggga tggacaaacc gtggtgctgg gtggcttgat
     5041 tcgtgaggat gtgcaggatg tcgaggacaa gattcctctc cttggtgatc tcccctgggt
     5101 gggccgcatg ttcaaatctc aggccgagga tcactttaaa cggaatctca tgattttcgt
     5161 gaccgccaaa ctcattgacc ctgccgggca acccatcaac accaaccttg gcggaggcgg
     5221 cgcggaaggc attggttcag ggcctagcac cgaccttttg ccgccagtca caggtctttg
     5281 atttcatagt ttgaccagat caggcaggcg ccccccccgg acgcctgcct gtttttttta
     5341 atcgctgact ttccatgaaa acctgggtca tcaccggcgg aatgggctgc ggaaagacct
     5401 tttttctgga tgtgctgttt cgctcagtgg aggagaaggt ggaggtgttc tcggcggacg
     5461 ccgaggtccg gcggctgatg agtttgccgg aaaccctgca aagcatcctg gaggtcttcg
     5521 gcccggaggc tgttattcag gaggctgggg cagacaaggt taatcgcgac tggctgcgcg
     5581 ggctggtttt ctcagatccc tcgcaccggt ccagcctgga agcgctgctt catcccggtg
     5641 tcctgtcctc cctggaaaag cggcggcggg aggcgcgcgt ggctggcgtc aatcttttcc
     5701 ttgcggaggt gcctttgcac tatgagattc aatccacagt atcagcggac ttggtcatcg
     5761 ttgtggcttc cagccgcgcg gtccaggtga ggcggttgat ggaaacccgg ggtctcgacc
     5821 aatcaatcat cgaaaagatg ttgagcgccc agttgcccat cgaagccaaa gtggagaagg
     5881 cggacgtggt catctggaat gatggccgcc ccgccgccct cgaagcccag gcgctgacgc
     5941 tggcaagaca gatccggcac gaatgaatcc catcgaaacc accccacctc cgcttgttcc
     6001 ctccgaacct gccgggaccg cttcggaggc ggccccccgc acattggctc ccgccgtgga
     6061 tggagagtca gcgcccgaag ccacggaggc atcggagcag ccgcagcctt ccgaaactgc
     6121 tccagcagag ccgggaggcg gcctgtctcc cgtggagaag cagtcccccg ccgccgagcc
     6181 tccattccct gtcgaaatct ccctcaacga cgtgcaggag atgccgttgt ccgcgcttca
     6241 ggagctcgcc tccagtgtcg gcttccggct caatgccagc cgcaccaagc accagctcat
     6301 ttacgacctg ctgtcctgga tggccgacca ccgcacccgc gtcaaagttg acggcatcct
     6361 cgaaatcgga gcggacaact acgggctgat acgttacccg aagtacagtt tcgccccgct
     6421 gcccgaggat gttttcatcc cgctgttcct ggtgcgcaaa ttccagctcc gtcccggcaa
     6481 ccgcatcacc ggctttgcgc gcgcgcccaa ggacagggac aagtacctgg ccattgaccg
     6541 catcctggag atagacggca tacccgtcga aacctgggag ccgcccgtgc atttcgacaa
     6601 gctcacggcc atgttcccca accagcggat catcctcgag ac
//
LOCUS       DAOMYJ010000262         6470 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Prosthecobacter sp. isolate AMR_MDS_5629
            NODE_4079_length_6470_cov_21.143882, whole genome shotgun sequence.
ACCESSION   DAOMYJ010000262 DAOMYJ010000000
VERSION     DAOMYJ010000262.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867850
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prosthecobacter sp. (wastewater metagenome)
  ORGANISM  Prosthecobacter sp.
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Verrucomicrobiaceae; Prosthecobacter.
REFERENCE   1  (bases 1 to 6470)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6470)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.30
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:34:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,742
            CDSs (total)                      :: 4,684
            Genes (coding)                    :: 4,676
            CDSs (with protein)               :: 4,676
            Genes (RNA)                       :: 58
            tRNAs                             :: 55
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6470
                     /organism="Prosthecobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4079_length_6470_cov_21.143882"
                     /isolate="AMR_MDS_5629"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1965333"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1935)
                     /locus_tag="PLP58_19510"
     CDS             complement(<1..1935)
                     /locus_tag="PLP58_19510"
                     /inference="COORDINATES: protein motif:HMM:NF019219.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1549 domain-containing protein"
                     /protein_id="HRJ10777.1"
                     /translation="MIRLLKPMSSLKAAALCCGAALTGIVPAAQAREVFFEDHIAPLL
                     ERSCRQCHNAETKRANLDLSSPATLLRGSDAGVVVKAGDPKHSLLYELVHSGEMPRKG
                     DKLTHDEVALIAEWIEGGARFRLAPVAVENGPRKHDILPVLLLRCTACHGAELQRGGL
                     DLRFAENMLQGGQSGPAVVPGKPDESLAIQRVEKGLCPPQDQLLKYFVERPGGTEVEK
                     LRDWVAAGASMRETPPDIADGKPDPLVTDEDRKHWAFQPLPAVVPVPASPDGLPMRPV
                     DAFIHEKLRAAGLDFSPEAERHSLIRRAYFDLTGLPPSTAELARWMEHLSDGWFAEMV
                     DHLLASPHYGERWGRFWLDVAGYSDSEGGTTEDPVREVAWKYRDYVIRAFNDDKPWDR
                     FLHEQLAGDELADHTDPAKITDEVTENLIATGFLRMGVDQTGSRTMNFVPERLGVISD
                     AIQVVGSGILGLTLDCARCHSHKYDAIPQRDYYRLKAVFQGAFDEHDWMTWKTRKLDT
                     ASPEAMARHKEVNGPLEKEIKALETTRTAALKKLQDEIYKQRWPALDKAGQEEILAAV
                     KANAIRRTLRQVELVKRYETEFRPVEAVLVKERPAFADELRGFDEKLAALRARLLPPL
                     TVRALWDRGRPSPTYLLSRGE"
     gene            2244..3065
                     /locus_tag="PLP58_19515"
     CDS             2244..3065
                     /locus_tag="PLP58_19515"
                     /inference="COORDINATES: protein motif:HMM:NF024242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HRJ10778.1"
                     /translation="MNTRTTQLQARQRFIENLAGSDLFRHYQTAFHTLTGLPLSLRAL
                     EDEEHVEEVVTRRGVAGVVHTLIPVKVGKNPVALLETGHVRLEPANADTFAPLASALL
                     DDDRSPAEIRSAKVHFEQAPMMAPARYDAAVCLLRSFAIQLGESAHRLLFAHATHEPE
                     AVRNAKIFIHAHLVEPMTLEDVAKAVNVSPFHFCKVFKRATGLTFTDFVNRARVEKAK
                     RMLMRPDARITEVAYDVGFQSLSHFNRSFRRIASESPTEYRGRMKNERGGVLAAG"
     gene            complement(3134..3409)
                     /locus_tag="PLP58_19520"
     CDS             complement(3134..3409)
                     /locus_tag="PLP58_19520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009965621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HRJ10779.1"
                     /translation="MNKAELTELVQKALGADTSKRAASDALEAVLESIAKGVKKDGSV
                     QLIGFGTFKVAKRAARTGRNPKTGESMKIKASKSVRFVPSSSLKGSL"
     gene            3545..4090
                     /locus_tag="PLP58_19525"
     CDS             3545..4090
                     /locus_tag="PLP58_19525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009965622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HRJ10780.1"
                     /translation="MSRSLLKNFLLSSTAPRAHPTVFTAPGAVVLGAVELAEESSIWF
                     GSVLRGDINRIVVGARSNVQDGSVLHVSDDHPCVLGALVTVGHRAVVHACEVGDETLV
                     GMGAIILDGAVIGPRCIIAAGALVPKGMRVPEGSLVMGSPARVTRPLTREEMDANTRL
                     ARKYVELARRYLGLDAWEPEA"
     gene            4145..4894
                     /locus_tag="PLP58_19530"
     CDS             4145..4894
                     /locus_tag="PLP58_19530"
                     /inference="COORDINATES: protein motif:HMM:NF014618.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional nuclease family protein"
                     /protein_id="HRJ10781.1"
                     /translation="MNREVIEIQVRAVLPLEGSFAVFLGNADKTFVIYVDENVGTAIS
                     MFMRGVPKDRPLTHDLLGSVLQAFGARVERVIINHVQGDVFHARLILSAENEIHQRKV
                     VEIDARPSDSIAMAVQQGAPLFVARKVLDSVQDVSATLAEIEKRGVEQTLPGAASEDD
                     ESGPEAEAETAGDEDDDDDGDDDDDDDDDVDDDDLEEIDPDSLDLDDLLADKDSDDDD
                     DDDDSGGTYDDGYQNLDDDDDEGEEWKKADG"
     gene            4945..>6470
                     /locus_tag="PLP58_19535"
     CDS             4945..>6470
                     /locus_tag="PLP58_19535"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012420888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004329 - formate-tetrahydrofolate ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HRJ10782.1"
                     /translation="MVPDISTVAHKLGLPEVHLQHHGRDKAKVSLKALEGRSARGKLI
                     LVSAITPTPAGEGKTTVSIGLAQGLKKIGRSVALALRQPSMGPVFGRKGGATGGGRST
                     IQPAESINLHFTGDFHAITSAHNLLAAVMDNQLFNKQTRLSPDGVLWKRVLDVNDRAL
                     RSLRTSAGGPTERATGFDITAASEVMAILCLSESLADLRARLDRIVMGFTADGAPVFA
                     KECHVTGAMLALLRDAMMPNLVQSVEGVPAFVHGGPFANIAHGCNSVLATRMALAHAD
                     YAVTEAGFAFDLGGEKFMHIKCRQSGLKPEAVVIVATVRALKMHGGATLDSLAQPNTA
                     AVERGLENLDAHLEAAALFQRPVIVALNRFTHDTDSELDLVRRHCQARGVPSAVADVF
                     GSGGEGAVALAETVLAALPAESPSLPFLYAATDDVEAKLSVIATQIYGAEGVTLTEEA
                     RARLAQFEKAGLSRLPLCMAKTQNSLSDDAKRPGRPRGFTLTVRDFEIANGAGFLVAL
                     T"
BASE COUNT         1141 a         2134 c         2045 g         1150 t
ORIGIN      
        1 ctcgccgcgc gaaagcaggt aggtgggcga gggccggccg cgatcccaca aggcgcgcac
       61 ggtgagcggc ggcagcaggc gcgcgcgcag cgcggcgagt ttttcatcga acccgcgcag
      121 ctcatccgcg aaggcggggc gctctttgac gagcacggct tccacaggcc tgaactcggt
      181 ctcgtaacgc ttcacgagtt ccacctggcg cagggtgcgg cggatggcgt tcgctttcac
      241 ggcggcgaga atttcctcct gcccggcctt gtccaatgcc ggccagcgtt gcttgtaaat
      301 ttcgtcctgg agctttttga gggccgctgt gcgcgtggtc tccagcgcct tgatttcttt
      361 ttccaagggg ccgttcactt ccttgtggcg ggccattgcc tcagggctgg cggtgtcgag
      421 cttccgcgtc ttccacgtca tccagtcgtg ctcgtcgaac gcgccttgga agacggcttt
      481 aagccggtag tagtcgcgct gcgggatggc gtcatacttg tggctgtggc agcgcgcgca
      541 gtcgagcgtg aggccgagga tgccggagcc gaccacctgg atcgcgtcac tgatgacgcc
      601 gaggcgctcg ggcacgaagt tcatcgtgcg cgaaccggtc tggtccacac ccatgcgcag
      661 aaagccggtg gcgatgaggt tttcagtgac ctcgtcagtg atcttggcgg ggtcggtgtg
      721 gtcggccagt tcatcgccgg cgagctgctc gtgcaggaag cggtcccagg gtttgtcgtc
      781 attgaaggcg cggatgacgt agtcgcggta cttccaggcc acttcgcgca cagggtcctc
      841 ggtggtgccg ccttcggagt cggagtagcc ggccacatcg agccagaagc ggccccagcg
      901 ctcgccgtag tggggagagg cgagcaagtg atccaccatc tcggcgaacc agccgtcgct
      961 caagtgctcc atccagcgtg cgagttcggc ggtggaaggc gggagtccgg tgaggtcgaa
     1021 gtaggcgcgg cggatgaggc tgtggcgctc cgcctcgggt gaaaagtcga gcccggcggc
     1081 gcgcagcttt tcgtgaatga aggcatccac cgggcgcatg ggcaggccgt ccggcgatgc
     1141 tggcacgggc acgacggcgg gcaggggttg aaaggcccag tgcttgcggt cctcgtccgt
     1201 gaccagcggg tcgggtttgc cgtcggcaat atcgggcggc gtctcgcgca tggaggcgcc
     1261 tgcggcgacc caatcgcgca gcttctccac ctcggtgccg ccggggcgct cgacgaagta
     1321 tttcaaaagc tggtcctgcg gcgggcacaa acccttttcg actcgctgga tggcgaggct
     1381 ttcgtccggc ttgccaggca cgacggcggg gccggactgg ccgccttgga gcatgttttc
     1441 ggcgaagcgc aggtccaagc cgccgcgctg caactccgct ccgtggcagg ccgtgcagcg
     1501 cagcagcagc acgggcagga tgtcgtgttt gcgcggtcca ttctccaccg ccaccggcgc
     1561 caggcggaaa cgcgcgccgc cctcgatcca ctcggcaatg agcgccacct cgtcgtgagt
     1621 gagcttgtcg cccttgcgcg gcatctcgcc gctgtggacg agctcgtaga gcaggctgtg
     1681 cttggggtcg cccgccttca cgaccacgcc cgcgtcgctg ccgcgcagga gggtggcggg
     1741 gctggagaga tcgagattgg cgcgtttggt ctccgcattg tggcactgcc ggcaagagcg
     1801 ctccagcagc ggggcgatgt ggtcctcgaa gaacacctcc cgcgcctgcg ccgcaggcac
     1861 gatgccggtc aaagccgcgc cgcagcagag cgcggcggct ttcagggatg acatcggctt
     1921 gaggagtcgg atcacggaaa caaataacgt ctttgcccgg cgctgtttgt ctgacgcgct
     1981 cgaatcctgg cgttttcaca gcgatccacg gcctttcaaa atcaggaaac accttgagtt
     2041 ggagagaatg gtggaggcgc gggttggacg cgaaaaccgg cgcatgacgc ccttacgagc
     2101 cggagcgagg cgcgcacggc taatggtttc cactatgcct tgattgcggt gagtcatcat
     2161 gaattgcgcc gtagccgcca ccaatagcgg gggagtcagg cggagcctag ccttcgtccg
     2221 tcacaccact cgacaacacc gccatgaaca cacgcaccac ccaactgcaa gcacgtcaac
     2281 gtttcatcga aaatctggca gggagcgatc tcttccgcca ctaccaaacc gcgttccaca
     2341 ccctcaccgg cctgcccctt tccctccgcg ccctggagga cgaggagcat gtggaggagg
     2401 tggtcacgcg ccggggcgtc gccggagtgg tccacaccct cattcctgtg aaagtgggaa
     2461 aaaatcccgt ggccctgctc gagaccgggc atgtgcgcct cgagccagcc aatgcggaca
     2521 ccttcgcccc gctggccagc gccctgctcg acgacgaccg cagcccggcc gagatccgct
     2581 ccgccaaggt tcacttcgag caggctccca tgatggcccc cgcgcgttat gacgcggccg
     2641 tgtgcctgct gcgctccttc gccatccagc tcggcgagag cgcccaccgg ctgctcttcg
     2701 cccacgccac gcacgagccc gaggcggtgc gcaacgccaa gatcttcatc cacgcccacc
     2761 tcgtcgagcc gatgacgctc gaggacgtgg caaaggcggt caacgtgagc ccctttcatt
     2821 tctgcaaggt cttcaagcgg gccaccggcc tcaccttcac cgactttgtg aaccgagccc
     2881 gtgtggagaa agccaagcgc atgctcatgc gccccgacgc gcgcatcacc gaggtggcct
     2941 atgatgtcgg cttccagagc ctgtcgcact tcaaccgcag tttccgccgt atcgccagcg
     3001 aatcgccgac cgagtaccgc gggcgcatga aaaacgagcg cggcggtgtc cttgccgccg
     3061 gctgagagat cgaaaaagaa aaaccccgcg cccggaagag cgcggggttt ttttcgaatg
     3121 aaccagagtc agatcacagc gagcctttca gcgagctgga aggcacgaag cgcacgctct
     3181 tcgaggcctt gatcttcatg gactcgccgg tcttgggatt gcggcccgtg cgggcggcgc
     3241 gcttggcgac cttgaaggtg ccaaagccga tcagctggac actgccgtcc tttttgacgc
     3301 ctttggcgat ggattcgagc acggcttcaa gggcgtcgga agctgcgcgc ttcgaggtgt
     3361 cggcgccgag cgccttctgg acgagttcgg tgagttctgc tttgttcatg gtggtgggtt
     3421 gatggtgttg atgatttttg ctttttccgc cgggatttct cgcggaattg ccgcgattta
     3481 gacgccagcc agccagtcca gtcaatcata aaaatcccgc ctaccgcccg caaattcaaa
     3541 gatcatgagc cgcagtcttc tgaaaaactt cttgctttcc tccaccgcgc cgcgcgcgca
     3601 tcccacggtt ttcaccgcgc cgggagcggt cgtcctaggc gcggtggagc tggccgagga
     3661 gtcgagcatt tggtttggca gcgtcctgcg cggcgacatc aaccgcatcg tcgtcggcgc
     3721 gcgcagcaac gtgcaggacg gcagcgtgct gcatgtgagc gacgaccacc cctgcgtgct
     3781 cggcgcgctc gtcaccgtgg gccaccgcgc cgtggttcat gcctgcgaag tgggggatga
     3841 aacacttgtg ggcatgggcg ccatcatcct cgacggcgcg gtcatcggcc cgcgctgcat
     3901 catcgccgct ggcgcgctcg tgccgaaagg catgcgcgtg ccggaaggca gcctggtcat
     3961 gggttccccc gcgagagtga cgcggcccct cacccgggag gagatggacg ccaatacgcg
     4021 cctcgcccgc aaatatgtgg agctggcgcg ccgctacctc gggctggatg catgggagcc
     4081 tgaagcataa attcacggtc agggggttgc ctttgcccgg cgcggagttt tagtcatggc
     4141 agtcatgaac cgcgaagtca tagaaatcca ggtgcgcgcc gtcctcccgc tggagggcag
     4201 ttttgccgtg ttcctcggga acgcggacaa gaccttcgtg atctatgtgg acgagaacgt
     4261 gggcacggcc atctccatgt tcatgcgcgg agtgcctaag gaccgcccgc tcacgcatga
     4321 tctgctgggc tctgttctcc aggcttttgg cgccagggtc gagcgggtca tcatcaatca
     4381 tgtgcagggt gatgtttttc acgcgcgtct gattctctcc gccgagaatg aaatccacca
     4441 gcgcaaagtc gtcgaaatag acgcgcggcc cagcgacagc atcgccatgg cggtgcagca
     4501 gggcgccccg ctgttcgtgg cgagaaaggt gctggattcg gtgcaggatg tcagcgccac
     4561 cctggcggaa atcgaaaaac gcggtgtcga gcaaaccctg ccgggcgcag cgtccgaaga
     4621 tgatgaatcc ggcccggagg cagaagccga gactgccggc gacgaagacg atgatgatga
     4681 tggtgacgat gacgatgacg acgacgacga cgttgatgat gatgatctcg aagagataga
     4741 tcccgacagc ctcgacctcg acgacttgct tgctgacaag gacagcgatg atgatgatga
     4801 tgatgacgac agcggcggca cctacgacga cggttatcaa aacctcgacg atgatgatga
     4861 cgagggcgag gaatggaaaa aagcggacgg ttgagaaaac gcctttcccc ggaaggtcag
     4921 ccgtccattt tccaacaccc ccgcatggtt cctgacatct ccaccgtcgc tcacaaactc
     4981 ggtttgcccg aggtccacct gcagcaccat ggccgtgaca aggccaaggt ttccctcaaa
     5041 gccctcgaag ggcgctccgc gcgcggcaag ctcatcctcg tctccgccat cacgcccacc
     5101 cccgccggcg aaggcaagac caccgtctcc atcggcctcg cccagggtct caaaaaaatc
     5161 ggcagaagcg tcgcgctagc cctccggcag ccctccatgg ggccggtctt cgggcgcaaa
     5221 ggcggcgcca ccggcggcgg gcgcagcacc atccagccgg cggagtccat caatctccac
     5281 ttcaccggcg acttccacgc catcaccagc gcgcacaacc tgctcgccgc cgtgatggac
     5341 aaccagctct tcaacaaaca gacgcgcctt tccccggacg gcgtgctgtg gaagcgcgtg
     5401 ctcgatgtca acgaccgcgc cctgcgctcc ctgcgcacct ccgccggcgg ccccaccgag
     5461 cgcgccaccg gctttgacat caccgccgcc tccgaggtca tggccatcct ctgcctcagc
     5521 gaatccctgg ccgacctgcg cgcgcggctc gaccgcatcg tcatgggctt cactgcggat
     5581 ggcgctcccg tctttgctaa agaatgccat gtcaccggag ccatgctcgc cctgctgcgg
     5641 gatgccatga tgccgaatct tgtccagtct gtcgaaggcg tgcccgcctt tgtccacggc
     5701 ggcccgtttg ccaacatcgc ccacggctgc aactccgtgc tcgccacccg catggcgctg
     5761 gcgcacgccg actacgctgt caccgaggcg ggcttcgcct ttgatctcgg cggtgaaaaa
     5821 ttcatgcaca tcaagtgccg ccagtccggc ctcaaacccg aagccgtcgt catcgtcgcc
     5881 accgtgcgcg cgctgaagat gcacggaggc gccacgctcg acagcctggc gcagcccaac
     5941 accgccgccg tggagcgcgg gctggaaaat ctcgacgccc atctcgaagc tgcggcgctg
     6001 tttcagcggc cggtcatcgt cgcattgaac cgcttcactc acgacactga ttccgagctt
     6061 gatctcgtgc gccggcactg ccaggcgcgc ggcgtgccca gcgccgtggc ggatgtcttc
     6121 ggcagcggtg gagaaggcgc ggtggccctg gcagagaccg tcctcgccgc gctgcccgcc
     6181 gaaagcccgt ccctgccctt cctctatgcc gccaccgatg acgtggaggc aaagctcagc
     6241 gtcatcgcca cccagatcta tggcgccgag ggcgtcactc tcaccgaaga ggcccgcgcc
     6301 cggctcgcgc agtttgaaaa agccggcctc tcccgcctgc ccctgtgcat ggccaaaacg
     6361 caaaactccc tctccgacga cgccaaacgc cccggccgcc cgcgcggttt caccctcacc
     6421 gtgcgcgact tcgaaatcgc caacggcgcc ggcttcctcg tcgccctcac
//
LOCUS       DAOMYJ010000263         6420 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Prosthecobacter sp. isolate AMR_MDS_5629
            NODE_4126_length_6420_cov_19.856245, whole genome shotgun sequence.
ACCESSION   DAOMYJ010000263 DAOMYJ010000000
VERSION     DAOMYJ010000263.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867850
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prosthecobacter sp. (wastewater metagenome)
  ORGANISM  Prosthecobacter sp.
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Verrucomicrobiaceae; Prosthecobacter.
REFERENCE   1  (bases 1 to 6420)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6420)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.30
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:34:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,742
            CDSs (total)                      :: 4,684
            Genes (coding)                    :: 4,676
            CDSs (with protein)               :: 4,676
            Genes (RNA)                       :: 58
            tRNAs                             :: 55
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6420
                     /organism="Prosthecobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4126_length_6420_cov_19.856245"
                     /isolate="AMR_MDS_5629"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1965333"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(148..1431)
                     /gene="serS"
                     /locus_tag="PLP58_19540"
     CDS             complement(148..1431)
                     /gene="serS"
                     /locus_tag="PLP58_19540"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012421138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004828 - serine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006434 - seryl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HRJ10783.1"
                     /translation="MLDIRQIRENPGLVKTRLAARGSEWPALVDELLALDTARRAAET
                     ERQHLQAERNRISKEIGIGRKNGLDTSAQEAEVRGIGERIEQTGREADAADEKQRALL
                     LDLPNLPHNACPAGAGAEDNPVVREWGAKPEFGFTPRDHTLLGQSLGLFDFEAGAKIS
                     GSAFAVYRGLGARLERALINFLLDLHTTKHGYVEISPPLLVRPECLVGTGQLPKFGDQ
                     VYHSATDDLYLIPTAEVPVTNLHREEILKPAQLPVRYAAYTPCFRREAGSAGLGTRGL
                     IRMHQFDKVELVKITTPETSMDELESLTADAEAVLQKLGLHYRVIELCTGDLGFGSAK
                     TYDIEVWAPGQGAYLEVSSCSNFGDFQARRMNLRHKDEEGKNRFCHTLNGSGTALARL
                     FVALVESCQQDDGSILIPEALRPWFGAEKIAAAQA"
     gene            complement(1533..5192)
                     /locus_tag="PLP58_19545"
     CDS             complement(1533..5192)
                     /locus_tag="PLP58_19545"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HRJ10784.1"
                     /translation="MASSSQSRTSSAWVPPTAEELQKLLPQYEITAQLGRGGMGAVYR
                     GRQTTLDRPVAIKILSNALDEADASFAERFKNEARSMAKLAHPGIVGVYDFGETESGL
                     LYIVMEFIEGTDVAKMISEQGRLPVAHALAITAHVCDALRFAHDRGVIHRDIKPANIM
                     VGYDGVVKVADFGLAKMSAGGETAGLTQSGMALGTLHYMAPEALMLGSSADHRADIYA
                     VGVMLYQMLTGKVPHGMFELPSRQVPDLDPRYDDIIARAMREDRELRYQQIAELRRDL
                     DTMLTQPVVKVEEKAEKAPAALPTKDRPQRPVGRPASPPKPEPSPEPPAKSKSSGGGM
                     IWAVVGGVVVLGGLAYVLMGGSGKDDSGDKAVAKPSQPAPAEQKPVTVQQPPAPKKAA
                     PVIASKSPTPSPVPAPPVKTPAPAVSSKPVASPAPVVEPKLEDPLIALVKLPGFQTRV
                     ANYQKARATQLGELTAKYQNALAAARQSATQSGSLTHVVAMDQAIQRATTHANTLEKL
                     PAVLKVEPLPPLPALDDSTPDSLKKLRGIFDGETERIEEELQAALLKSLNALQVSLVK
                     SGDLDNARVVEVYRKQLDGAATIAPTGPFTNSLGMKFVPLKIGSGPSAGTTLFFSVWE
                     TRVADYAAFAAENEVPGNWRGRKDQGIEQTPDHPVLEVTAELASKFCDWLSQKEKMNC
                     RLPTSEEWDWAAGIRWAGQTEQELATKRGQRPWWKPGQTSKEVANFGLGLVDDFPFTA
                     PVGSFPPNEQGLFDIFGNVWEWVKSGDNRHLARGGGWGNNNWFTLGKEEPLPGFAGKA
                     TAMGFRVVVDSPERQPPVVAVATPPTTTPAPSPAPAPAADHVLFNGRDLTGWQTANPS
                     AWKVENGALVCRGADSLLAHPLSSHNIDLTGEFRVSSKASGGIVFYATSANPDEGWEV
                     PIVGSAVRADVLTGGMVDHKTGAKTTPVATQSKDGEWQSFRFLVSKVLSKIWVGGPGT
                     NHRMGRPITQPTHLILRASVADGEIAFRNLVVKNTLDYPGINLGDPGTGPEWTPLITD
                     AQLSQWKISGNSKDFAFEDGALRIQVTDKGPSYLYLTFGQGRPPPIKSFDLMFKARAT
                     GDANSGIYFHVDPSRWQSLGHPSTGYEINLANGGPPDKLTGAVYNIAPPLIDLPSQSD
                     WFDVRICVKDNRVLCLINGKIAQDFVEPADRSTLPVLASQKDRGFRKSGGAIAIQANS
                     KDAFFIKDMRFKSLD"
     gene            complement(5411..>6420)
                     /locus_tag="PLP58_19550"
     CDS             complement(5411..>6420)
                     /locus_tag="PLP58_19550"
                     /inference="COORDINATES: protein motif:HMM:NF012546.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PhoPQ-activated protein PqaA family protein"
                     /protein_id="HRJ10785.1"
                     /translation="LRKLTTPRRASAGTSPKAALAMDLNGSRPPAPEYDPQTGLALGN
                     GHRGQRTRLPKGGLEHGHPQKFGSIGGTHLDDWPYHAAASVIRAHSLLRGFPEVDAER
                     TAVTGISWGGYTTCLAASLDDRFKAAVPVYGCGFLHEGESVQKPSIDALGDRRMDWVR
                     AYDPGSLLPRAKVPLLFVNGTNDKHYVLDSYMKSYAVVTAPKQLRIQVNMPHGHPPGW
                     APQEIGLFIDSHCVGGTPLPVPGQPVIEGEKVTLVCDSATALKSAALHYTTDTGPRSE
                     RKWTTVPAMLAFVTATTGGTTAYRTRITAPAPPPEANTWFLSVTDERDAMVSTTVQFA
                     P"
BASE COUNT         1025 a         1976 c         2181 g         1238 t
ORIGIN      
        1 ccccacgttc acatccacgt tcgcatattc gcaaatttcg tcggggatgt cctccacccg
       61 ccagccggcg ccgagcatgg cggcgacttc ggcggtcttg tgcgcgttcg aggtggcgag
      121 cagcaggcgg ggcatgggcg cggcggttca ggcttgggcg gcggcgatct tttccgcgcc
      181 gaaccagggg cgcagagcct ccgggatgag gatggagccg tcgtcctgct ggcagctctc
      241 caccagggcc acgaacagcc gggccagcgc ggtgccggag ccgttgagcg tgtggcagaa
      301 gcggtttttt ccctcctcgt ccttgtggcg caggttcatg cgccgcgcct ggaagtcgcc
      361 gaagttcgag cagctcgaca cctccaggta ggcgccctgg ccgggcgccc agacctcgat
      421 gtcataggtc ttggccgagc cgaagccgag atcgccagtg cacagctcga tgacgcggta
      481 atgcaggccg agcttttgca gcaccgcctc ggcgtcggcg gtcaggcttt ccagctcgtc
      541 catgctcgtc tccggcgtgg tgatcttcac cagctccact ttgtcgaact gatgcatgcg
      601 gatcaagccg cgcgtgccca ggccggcgct gcccgcctcg cgccggaagc agggcgtgta
      661 ggcggcgtag cggaccggga gttgcgcggg tttcaggatc tcctcgcggt gcaggttcgt
      721 caccggcacc tcggcggtgg ggatgaggta aagatcatcc gtggcgctgt gatagacctg
      781 gtctccgaac ttcggaagct gccccgtgcc caccaggcac tccggcctga cgagcagcgg
      841 cgggctgatc tccacataac cgtgcttcgt ggtgtgcagg tcgagcagga aattgatcag
      901 cgccctttcc agcctggcgc ccaggccgcg atacaccgcg aaggcgctgc cgctgatctt
      961 cgcgcccgcc tcgaagtcga aaaggccgag cgactgcccc agcagcgtgt gatcccgcgg
     1021 cgtgaagccg aactccggct tcgcgcccca ttcgcggacc acgggattgt cctccgcgcc
     1081 cgcgccggcg gggcaggcat tgtgcggcag gttcggcagg tccagcagca gcgcgcgctg
     1141 cttttcatcg gcggcgtcgg cctcgcgccc ggtctgctcg atgcgctcgc cgatgccgcg
     1201 cacctccgcc tcctgcgcgc tggtgtccag gccgtttttc cgcccgatgc cgatctcctt
     1261 gctgatgcgg ttgcgctccg cctggaggtg ctggcgctcc gtctccgccg cgcgccgcgc
     1321 cgtgtccagc gccagcagtt cgtccaccag cgccggccat tcggagccgc gggcggcgag
     1381 gcgggttttg acgaggccgg ggttttcgcg aatctggcgg atgtcgagca taagggcgcg
     1441 gagcctagcg cgaatgcgcg ggctgccaaa ggcaaaatca ccgcctgcaa agcggcgcgg
     1501 gctttgcctc cggccgcgcc cgctttccgg gttcaatcca gggacttgaa gcgcatgtcc
     1561 ttgatgaaaa aggcgtcctt ggagttcgcc tggatggcga tggcgcctcc ggatttgcgg
     1621 aatccccggt ccttttggct ggcgaggacg ggcagcgtgg agcggtcggc gggttcgacg
     1681 aagtcctggg cgatcttgcc gttgatgagg cagaggacgc ggttgtcttt gacgcagatg
     1741 cgaacatcga accagtcgct ttgactgggg aggtcaatga ggggcggggc gatgttgtaa
     1801 acggcgccgg tgagcttgtc gggagggcct ccgttggcga gattgatctc atacccggtg
     1861 gagggatggc cgagcgactg ccaccgggag gggtcaacgt ggaagtagat gccggagttg
     1921 gcatctccgg ttgcccgggc cttgaacatg aggtcgaagc tcttgatggg cggaggccga
     1981 ccctgcccga aggtgaggta aaggtaggat ggtcccttgt cggtgacttg gatccgaaga
     2041 gcgccatcct caaacgcaaa gtccttcgag ttgccgctga tcttccactg gctgagttgg
     2101 gcgtcggtga tgaggggcgt ccactcggga cccgtgccgg ggtcacccag attgatcccg
     2161 ggataatcca gcgtgttttt cacgacaaga ttgcggaaag cgatctcacc atcggcgacc
     2221 gaggcacgca ggatgagatg ggtgggctgg gtgatgggac ggcccatccg atggttggtg
     2281 cccggaccac cgacccaaat cttgctcagg accttcgaca ccagaaatcg gaagctttgc
     2341 cactcgccat ccttggattg agtggcgaca ggcgtcgttt tggcccccgt cttgtgatcc
     2401 accatgccgc cggtcaggac atctgcacgg actgcggaac ccacgattgg cacctcccac
     2461 ccctcatccg gattggcaga ggtggcatag aacacgatgc ctccgctggc ctttgaagaa
     2521 acgcggaact cgccagtcag gtcaatattg tgcgaggaaa gcgggtgggc gaggaggctg
     2581 tcggcaccgc ggcagacgag ggcgccattc tccaccttcc atgcagatgg attggccgtc
     2641 tgccagccgg tcaggtcacg accgttgaaa agaacatgat cagctgcggg agcgggtgcg
     2701 ggagacggcg caggagtggt ggttggaggc gtggcgacag cgacgactgg cggctgcctc
     2761 tcgggtgagt ccaccaccac acgaaatccc atggccgttg ccttgccggc aaatcccggc
     2821 agcggctctt cttttcccag agtgaaccag ttgttgttgc cccagcctcc tccgcgggcg
     2881 agatggcggt tgtcgccaga ctttacccac tcccacacgt tgccgaagat gtcgaacaga
     2941 ccttgttcgt ttggcgggaa ggatccgacc ggtgcggtga agggaaagtc atcgaccagc
     3001 cccagcccaa aattggccac ttcttttgag gtttgtccgg gtttccacca tggcctctgt
     3061 cctctcttcg tcgccagttc ctgctcggtc tgacccgccc aacggattcc agcggcccag
     3121 tcccactcct ccgaagttgg cagccggcaa ttcatcttct ccttttggga aagccagtca
     3181 cagaacttgc tggccagttc agcagtgact tcgagcacag gatgatccgg tgtctgttca
     3241 atcccctgat cctttctgcc acgccagttg ccaggcacct cgttttccgc cgcgaaggcg
     3301 gcatagtccg ccacccgcgt ctcccacaca ctgaagaaga gcgtcgttcc cgcactcgga
     3361 ccgctgccga tcttcagggg cacgaatttc atgccgaggc tgttggtgaa cggacctgtg
     3421 ggtgcgatgg tggcggcgcc gtcaagctgc ttgcggtaaa cttccaccac gcgcgcgttg
     3481 tcgagatcgc cagatttgac gagggagacc tggagggcat tgagcgattt aaggagcgcg
     3541 gcttgcagtt cctcttcgat cctttcggtt tcaccgtcga agattccccg caacttcttc
     3601 agggagtcgg gcgtgctgtc atcgagcgcg ggcagcggcg ggagcggctc gaccttgaga
     3661 acggcaggca gcttttcgag cgtgttcgcg tgggtggtgg cgcgctggat ggcctgatcc
     3721 atcgccacca catgggtgag gctgccactt tgggtggcgc tctgcctggc ggcggcgagg
     3781 gcgttttgat atttggccgt caactcgccc agctgggtgg cacgcgcctt ttgataattg
     3841 gccacgcgcg tttgaaagcc gggaagcttt accagcgcga tgagcggatc ttccagtttg
     3901 ggttccacaa caggcgctgg agaagccacc ggcttcgaac tcaccgctgg cgcgggtgtt
     3961 ttcaccggag gtgccggcac gggcgagggc gtcgggcttt tggacgcaat caccggagcc
     4021 gccttcttgg gagccggagg ttgttgcacc gtgacgggtt tttgttctgc gggagctggt
     4081 tgggatggtt tagccacggc tttatcaccg gagtcgtcct tccccgaacc gcccatgagc
     4141 acatatgcca gcccacccag cacgacgact cctcccacca ccgcccaaat catgccgccg
     4201 ccggatgact tggatttggc aggaggctct ggcgaaggtt ccggctttgg tggagacgcg
     4261 ggccggccca cgggacgctg cggacggtct ttggtcggca gggccgcggg cgctttttcc
     4321 gccttctcct ccaccttcac caccggctgc gtgagcatcg tgtcgagatc acggcgcagt
     4381 tcggcgatct gctggtagcg caattcgcga tcctccctca tcgcgcgggc gatgatgtcg
     4441 tcgtagcgtg gatcaagatc cgggacctgc cttgacggca attcaaacat gccgtgcggc
     4501 actttgccgg tgagcatctg gtaaagcatc accccgactg cgtagatgtc cgcgcggtga
     4561 tcggcggagc ttcccagcat cagcgcctcc ggagccatgt aatgcagtgt tcccaaagcc
     4621 atgccgcttt gcgtcaggcc ggccgtttct ccacccgcgc tcatcttggc caggccgaag
     4681 tccgccacct tcaccactcc gtcataaccc accatgatgt tcgccggttt gatgtcccgg
     4741 tggatgacgc cccggtcgtg cgcaaaccgc agcgcatcgc aaacatgcgc cgtgatggcc
     4801 agcgcatgcg ccacgggcag gcggccctgc tccgagatca tcttcgccac gtcggtgccc
     4861 tcgatgaact ccatcacgat gtagagcagc ccgctttcgg tctcgccaaa gtcgtaaacc
     4921 ccgacgatgc ccggatgcgc gagcttcgcc atggagcgcg cctcgttctt gaagcgctcg
     4981 gcaaagctgg cgtccgcctc gtccagcgcg ttggagagaa tcttgatggc caccggccgg
     5041 tccagcgtgg tctgcctccc cctgtacacc gcccccatgc caccgcgccc gagctgggcg
     5101 gtgatctcat actgcggcag cagtttttgc aactcctccg ccgtgggtgg cacccatgcg
     5161 cttgaggtcc tgctttgtga agagctggcc atggcgcgca acctcgaatg aagccgggtg
     5221 gatggcaagc atggaaaaat gccttcgcgt caggcattgg gttttgaatt tgggcagggc
     5281 ggcattcacc cgaacggcag aaggcggggc cgcgcagccc gccgtctttc aggaggttca
     5341 tcaggaacgc aaggcccggc tcaaacggct ggcactgcga attcgggatg ccgcggtgaa
     5401 tgtgccgtgc tcacggcgcg aactgcacgg tcgtgctgac catggcgtcg cgctcgtcgg
     5461 tgacgctaag gaaccaggtg ttcgcctccg gcggcggggc gggcgcggtg atgcgggtgc
     5521 ggtacgccgt cgtgccgccg gtggtggcgg tgacgaaggc gagcatggcg gggacggtgg
     5581 tccacttgcg ctcgctgcgc gggccggtgt cggtggtgta atgcagggcg gcgcttttca
     5641 gggcggtggc gctgtcacaa acaagcgtga ctttttcccc ttcgatgacg ggctggccgg
     5701 gcacgggcag cggtgtgccg ccgacgcagt ggctgtctat gaagaggccg atctcctgcg
     5761 gcgcccagcc gggcgggtga ccgtggggca tgttcacctg gatgcggagc tgcttcggcg
     5821 cggtgacgac ggcgtagctt ttcatgtagc tgtcgagcac atagtgcttg tcgttggtgc
     5881 cgttgacaaa gagcagcggg actttggcgc ggggcagcag gctgccggga tcataggcgc
     5941 gcacccagtc catgcggcgg tcgccgaggg cgtctatgga gggcttttgc acggactcgc
     6001 cctcgtgcag gaagccgcag ccatagacgg ggacggcggc cttgaagcgg tcgtcaaggg
     6061 aggcggcgag gcaggtggtg tagccgcccc aactgatgcc cgtgacggcg gtgcgctccg
     6121 cgtccacctc ggggaagccg cgcaggaggg agtgggcgcg gatgacgctg gcggcggcgt
     6181 ggtagggcca gtcgtcaagg tgggtgccgc cgatgctgcc aaacttctgc gggtggccgt
     6241 gctcgagacc gcccttgggc aggcgggtgc gctggccgcg gtggccgttg cccagggcca
     6301 ggccggtctg cggatcatac tccggcgcgg gcgggcggga accgttcagg tccatggcca
     6361 gggcggcctt cgggctcgtg ccggcggagg cacgccgcgg ggtggttaat tttctcaaag
//
LOCUS       DAOMYJ010000264         6398 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Prosthecobacter sp. isolate AMR_MDS_5629
            NODE_4140_length_6398_cov_19.384834, whole genome shotgun sequence.
ACCESSION   DAOMYJ010000264 DAOMYJ010000000
VERSION     DAOMYJ010000264.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867850
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prosthecobacter sp. (wastewater metagenome)
  ORGANISM  Prosthecobacter sp.
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Verrucomicrobiaceae; Prosthecobacter.
REFERENCE   1  (bases 1 to 6398)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6398)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.30
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:34:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,742
            CDSs (total)                      :: 4,684
            Genes (coding)                    :: 4,676
            CDSs (with protein)               :: 4,676
            Genes (RNA)                       :: 58
            tRNAs                             :: 55
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6398
                     /organism="Prosthecobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4140_length_6398_cov_19.384834"
                     /isolate="AMR_MDS_5629"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1965333"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..308
                     /locus_tag="PLP58_19555"
     CDS             <1..308
                     /locus_tag="PLP58_19555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018290447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HRJ10786.1"
                     /translation="ALPAGEERWHMLPLTELAGRLARCAAFLGHDSGISHLAAACGLP
                     CLLLFGPTDPALWAPPQDGVRVLPSESGLLTGLDVGRVSRTARAWLEEITAKGKSVL"
     gene            339..971
                     /locus_tag="PLP58_19560"
     CDS             339..971
                     /locus_tag="PLP58_19560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009958236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside monophosphate kinase"
                     /protein_id="HRJ10787.1"
                     /translation="MADSVKARFRTLLILGAPGSGKGTQGLVLGQIPRFHHLACGDVF
                     RSLDTRTPLGQKFVQYSSRGELVPDDVTVALWRANITQRVDSHAFKPEIDLLVLDGIP
                     RNVEQARLMEADIEVLKVFHLSCPDRAELARRLRKRALKDNRFDDASEAVIQNRFATY
                     EAETKPILEHYAGARIVNIDASQSPARVMHDILGHVLQLPAWQEIERMNV"
     gene            987..1928
                     /gene="fmt"
                     /locus_tag="PLP58_19565"
     CDS             987..1928
                     /gene="fmt"
                     /locus_tag="PLP58_19565"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012420019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004479 - methionyl-tRNA formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HRJ10788.1"
                     /translation="MRLIFIGTGDIGLPALDWLIRASGHELAAVVTQPDKPAGRKLVL
                     TPPQVKVRAIEAGLPVLQPVKIRHAIEELRAFQADAAIVAAYGQLLSPEVLRVPRHGC
                     LNLHASLLPRHRGASPIQAAIREGDSETGITVMHMDEGLDTGDILLMEHTPILATDTG
                     GSLHDRLAALAPAALEHALALIESGKAPRIPQDAALATHCGKLRREDGRLDWTRPASE
                     LERLIRAYQPWPGTFTQTGGEKPATLKIHRAIAVPEADACPIPGTLLSADPKNGLVIA
                     CGRGFLRLDEVQAEGGKRLPASDFLRGHPMQPGTLLR"
     gene            complement(2463..3338)
                     /locus_tag="PLP58_19570"
     CDS             complement(2463..3338)
                     /locus_tag="PLP58_19570"
                     /inference="COORDINATES: protein motif:HMM:NF020039.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMP-30/gluconolactonase/LRE family protein"
                     /protein_id="HRJ10789.1"
                     /translation="MKAAFFTFALLAAPLAAQDTSLHEYLIEGEDWKEAASGYEFTDG
                     LCNDAAGNLYFTDVKAGKGIYKLDAATGSTSLFLDNLPGISGLHIGPDGRFYACHNRE
                     QRVIAISMKGEVEVLVTGVKCNDLVVSKKGHVYFTETPTQRVHWITPDKKHAVADEGR
                     VVKPNGITLSADEATLAVSEHGGKHVWAWQIQEDGSLTAGAPFMTMWLPVGKEAAAGD
                     GSTTESKGRYFVTTELGVQIFDPAGRLAGIIRKPVADGKVVSVGFGGKDHDILYVAAG
                     KSIYGRKVKVSGYFR"
     gene            complement(3387..4247)
                     /locus_tag="PLP58_19575"
     CDS             complement(3387..4247)
                     /locus_tag="PLP58_19575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007414949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase-fold protein"
                     /protein_id="HRJ10790.1"
                     /translation="MNHALLFASLALTLHAASQDDQYVLGPDSQVQPGVPQGRVTQMP
                     AWTDSKIFPGTTRDWWVYVPAQYAKDKPAAVMVFCDGAGFVKADGQFRAPVVFDNLIA
                     KGEMPVTIGIFIQPGVFPASNPKDKPRSNRSFEYDSLGDLYARFLLEEILPAVAKDHN
                     LSTDPEMRAICGNSSGGICAFTVAWERPDAFRRVVSHIGSFTNIRGGHVYPALIRKTD
                     KKPLKVFLQEGSNDLDNQFGNWPLANQDMAAALKFAGYDHKLVMGEGTHNGRHGAAIF
                     PDTMRWLWAK"
     gene            4404..5681
                     /locus_tag="PLP58_19580"
     CDS             4404..5681
                     /locus_tag="PLP58_19580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009958816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HRJ10791.1"
                     /translation="MQRPRLAIIGTGISGLGCAHFLHERFDLTLFEQTDHIGGHTNTV
                     TVDEDGREVPIDTGFMVFNHQTYPLLCRLFRELGVETRRTDMSFSLKHLPTGYEWNGG
                     SLGKVFGQRKNLASPRFWSFVLKIHRFNQETAAALDDPAFDRMTLREYVAARGYGRDF
                     LDLYIIPMGSAVWSTPPDLMLEFPAKTLMRFWFNHGFLGMETRHPWWTVVDGARAYVR
                     KIIPPFADRIRLRAPVARVERREGKAWVQPREGAPECFDKVILAGHAPQTLAILADPT
                     PLEAELLGCFKYQPNTATLHTDESFMPKTRRCWASWNYRVALDAQGRPDPSTHYWMNS
                     LQGVSDKKNYFVSINAADEINPAKVLRRIHYEHPLFDLRAVDAQKRLSGLNRLGPDQT
                     TYYCGAWFRHGFHEDGFMSAVHLCRDLLGREPW"
     gene            5973..>6398
                     /locus_tag="PLP58_19585"
     CDS             5973..>6398
                     /locus_tag="PLP58_19585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ10792.1"
                     /translation="MRKGGFLVLTRRVDGAGRLAAVEWSFAGVERVVRYDYDSHSRLE
                     QASFGLMNQPPQVSAGYHWLPGTRRVERLERPIAGSAQPLTGVFTHDAAGRLETLEWR
                     RGGTLYGSHEYGFDAAGRRQTETRQDGATLHYAYTPRGEL"
BASE COUNT         1097 a         2282 c         1957 g         1062 t
ORIGIN      
        1 cagcgctccc cgccggcgag gagcgctggc acatgctgcc gctcaccgag ctcgccggca
       61 ggctggcccg ctgcgccgcc tttctggggc atgacagcgg catctcccac ctcgccgccg
      121 cctgcggcct gccctgcctg ctgctgttcg gccccaccga ccccgccctg tgggcgccgc
      181 cgcaggatgg agtgcgtgtt cttccctccg aatccggcct tctcaccggc ctggacgtcg
      241 gccgcgtcag ccgcaccgcc cgcgcatggc tggaggaaat caccgccaaa ggcaagagcg
      301 tcttgtgaca ggcgcgtgac gtgctatgcc atgacctcat ggctgactca gtaaaagcac
      361 gtttccgcac cctcctcatc ctcggcgccc ccggcagcgg caaaggcacg cagggcctgg
      421 tcctgggcca aatcccgcgg tttcaccacc tggcctgcgg ggacgtcttc cgctccctgg
      481 acacccgcac cccgctcggc cagaaattcg tccaatactc cagccgcggc gagctggtgc
      541 cggatgatgt caccgtcgcc ctctggcgcg cgaacatcac ccagcgcgtg gacagccatg
      601 ccttcaagcc ggagattgac ctcctcgtcc tcgacggcat cccgcgcaac gtcgagcagg
      661 cccgcctcat ggaggccgac atcgaggtgc tcaaagtctt ccacctttcc tgccccgacc
      721 gcgccgagct ggcgcgccgc ctgcgcaaac gcgcgctcaa ggacaaccgc ttcgacgacg
      781 ccagcgaggc cgtcatccag aaccgcttcg ccacttacga ggccgagacc aagcccatcc
      841 tcgaacacta cgccggcgcg cgcatcgtca acatagacgc cagccagtca cccgcccgtg
      901 tcatgcacga catcctcggc cacgtcctcc agctccccgc ctggcaggag atcgagcgca
      961 tgaatgtgtg accccgcacc cgccccatgc gcctcatttt catcggcacc ggcgacatcg
     1021 gcctccccgc cctcgactgg ctcatccgcg cttcaggaca cgagcttgcc gccgtcgtca
     1081 cccagcccga caagcccgcc gggcggaagc ttgtgctcac gccgccgcaa gtcaaagtgc
     1141 gcgccatcga ggccgggctg cccgtgctcc agccggtgaa aatccgccac gccattgagg
     1201 aactgcgcgc cttccaggcc gatgccgcca tcgtcgccgc ctacggccag cttctctccc
     1261 ccgaagtgct gcgcgtcccg cgccacggct gcctgaatct gcacgcctcc ctcctgcccc
     1321 gccaccgcgg tgccagcccc atccaggccg ccatccggga gggcgacagc gagaccggca
     1381 tcaccgtcat gcatatggac gagggtctcg acaccggcga catcctgctc atggagcaca
     1441 cccccatcct cgccaccgac accggcggca gcctgcacga ccgcctcgcc gccctggcgc
     1501 ccgcggccct tgagcacgcc ctcgctctca tcgagtccgg caaagcgccg cgcatcccgc
     1561 aggacgccgc cctcgccacc cactgcggca agctgcgccg cgaggacggc cggctcgact
     1621 ggacccgccc cgcgtccgag ctcgagcgcc tcatccgcgc ctaccagccc tggccgggca
     1681 ccttcaccca gaccggcggt gaaaagcccg ccacactcaa aatccaccgc gccatcgccg
     1741 ttcccgaagc ggacgcctgc cccattcccg gcacgctcct gagcgccgac cccaaaaacg
     1801 gcctcgtcat tgcctgcggc cggggtttcc tccgtctcga tgaagtccag gccgagggcg
     1861 gaaaacgcct gcccgcctcc gacttcctgc gcggccaccc catgcagccg ggaacgctgc
     1921 tgcgctgagc cgcgccgcgt ggcaggcgcg aaaaagagaa aagcgccccg gagaaccggg
     1981 acgcttttgt gacccgcctg tgccgtccaa gacgagggct ctcgtatccc aaaggcgacg
     2041 aggtgggtgc cggcggacaa gggcgctgtc ttccagtgaa ggcatgacat gacatcccag
     2101 gccgccgtgc tcccggtgtc attgaaacgg gccgggccac tgcgtcttga tgacgaacac
     2161 cgcaggaaag tctgccgccc ggaccgtgcc cggcgcggcg aaaggaggag tctggcaaag
     2221 agcggatgga aagcgcgcgc gccctccacg aattctgttt tcagcataaa agatgccgga
     2281 accgccggca agactttcct gccgccggat gcaggcgcgg cagggcgttt tcatgcgcaa
     2341 gcgcagctcg ttggagttca agctttagct tgtaaagcgc gtgttttcac cagccccccg
     2401 cactcagcag cttgtggcat cgtatggaaa cgccctggca ggcgcggaca gaggcgcgcg
     2461 gctcagcgga agtagccgct gaccttcacc ttgcgcccgt aaatgctctt tccggcggcc
     2521 acatagagga tgtcgtggtc tttgccgccg aagccgacgc tgaccacctt gccatcggcc
     2581 accggcttgc ggatgatgcc cgccaggcgc ccggcggggt cgaagatctg cacgcccagc
     2641 tcggtggtga caaagtagcg cccctttgac tcggtcgtgg agccgtcacc cgccgcggcc
     2701 tccttgccca cgggcagcca catggtcatg aagggagcgc ccgccgtgag gctgccgtct
     2761 tcctggatct gccacgccca cacatgcttg ccgccgtgct cggagacggc cagcgtggcc
     2821 tcgtcggcgg agagggtgat gccgttgggc ttcaccacgc ggccctcgtc ggccacggcg
     2881 tgctttttgt ccggcgtgat ccagtggacg cgctgcgtgg gcgtctcggt gaaataaacg
     2941 tggccctttt tgctgaccac gaggtcgttg cacttcacac cggtgacgag cacctccacc
     3001 tcccccttca tgctgatggc gatgacgcgc tgctcgcggt tgtggcaggc gtaaaagcgc
     3061 ccgtccggcc cgatgtgcag gccgctgatg cccggcaggt tgtcgaggaa aaggctcgtc
     3121 gagcccgtgg cggcgtccag cttgtagatg cccttgccgg ctttgacatc ggtgaagtag
     3181 aggttccccg ccgcgtcatt gcagaggccg tcggtgaatt catagcccga ggcggcctcc
     3241 ttccagtcct cgccctcgat gaggtattcg tgaagggagg tgtcctgggc ggcgagaggc
     3301 gcggcgagca gcgcgaaagt gaaaaaggcg gctttcatag tggcaggggg aggaatcaat
     3361 gagactggaa aatcaaaagc aagccctcac ttggcccaca gccagcgcat cgtgtcgggg
     3421 aaaatggcgg cgccgtgcct gccgttgtgg gtgccctcgc ccatgacgag cttgtggtcg
     3481 tatccggcga acttcagcgc ggcggccatg tcctggttgg ccagaggcca gttgccgaac
     3541 tggttgtcga ggtcgttgct gccttcctgg aggaagacct tgagcggctt tttgtccgtc
     3601 ttgcggatga gcgcggggta cacatggccg ccgcggatgt tggtgaagct gccgatgtgg
     3661 ctgaccactc tgcggaaggc gtccggccgc tcccaggcca ccgtgaaggc gcagatgccg
     3721 ccgctgctgt tgccacagat ggcgcgcatc tcggggtcgg tgctgaggtt gtggtctttg
     3781 gccacagcgg gcaggatttc ctccagcagg aagcgcgcgt agaggtcgcc cagggaatcg
     3841 tactcgaagc tgcgattgct gcgcggcttg tccttcgggt tcgaggccgg aaacacaccc
     3901 ggctggataa agatgcctat cgtcaccggc atctcccctt tggcgatgag gttgtcaaaa
     3961 accaccggcg cgcggaactg gccgtcggct ttcacaaaac ccgcgccatc gcaaaacacc
     4021 atcacggcgg cgggcttgtc cttcgcatac tgcgccggca catacaccca ccagtcgcgc
     4081 gtggtgccgg ggaagatctt cgaatccgtc cacgccggca tctgcgtcac cctgccctga
     4141 ggcacgccgg gctgcacctg cgagtcggga ccgagcacat attgatcatc ttgagaggcg
     4201 gcatggaggg tgagggcgag ggaggcgaac agcagggcgt gattcataaa gagcgggaag
     4261 gaacggcagg ccgcgcgctt tcttgcccgc acaccttggg acgggctcga aacacgcgcg
     4321 gatttccagg aatccatttc cacgcgcgcg gcgaattgac tccctctgtc cgcgccatcc
     4381 cggagcggcc ccactccgct cccatgcaac gtccccgtct cgccatcata ggcaccggca
     4441 tttccggcct cggctgcgct cattttctcc atgagcgctt tgatctcacc ctcttcgagc
     4501 agaccgacca catcggcggc cacaccaaca ccgtcaccgt ggacgaggac gggcgggagg
     4561 tgcccattga caccggcttc atggtcttca accaccagac ctatcccctg ctctgccgcc
     4621 ttttccgcga gctcggcgtg gagaccaggc gcacggacat gtccttcagc ctcaagcacc
     4681 tgcccaccgg ctatgaatgg aacggcggca gcctcggcaa ggtcttcggc cagcgcaaaa
     4741 acctcgccag tccgcgcttc tggagctttg tgctcaaaat ccaccgcttc aaccaggaga
     4801 ccgccgccgc cctcgacgac cccgccttcg accgcatgac gctgcgcgag tatgtggccg
     4861 cgcgcggtta cgggcgggat ttcctggacc tctacatcat ccccatgggc tccgccgtct
     4921 ggtccacgcc gcccgatctc atgctcgagt tccccgccaa gacgctcatg cgcttctggt
     4981 tcaaccacgg cttcctcggc atggagacgc gccacccctg gtggaccgtg gtggacggcg
     5041 cgcgcgccta tgtgcggaaa atcatcccgc ctttcgccga ccgcatccgc ctccgcgcgc
     5101 ccgtggcccg tgtcgagcgg cgggagggca aagcctgggt ccaaccgcgc gagggcgcgc
     5161 ccgaatgctt cgacaaggtc atcctcgccg gccacgcccc gcagacgctc gccattctgg
     5221 cggacccgac gcctctggag gcggagctgc tcggctgctt caaataccag ccgaacaccg
     5281 ccaccctgca caccgacgag tccttcatgc ccaaaacccg ccgctgctgg gcctcctgga
     5341 actaccgcgt ggcgctcgac gcccagggcc gcccggaccc cagcacgcat tactggatga
     5401 actccctgca gggcgtctcc gacaagaaga actacttcgt cagcatcaac gccgccgatg
     5461 agatcaaccc cgccaaagtc ctccgccgca tccactacga gcacccgctt ttcgacctcc
     5521 gcgccgtgga cgcgcaaaaa cgcctgtccg ggctgaaccg cctcggcccg gaccagacca
     5581 cctactactg cggcgcctgg ttccgccacg gcttccacga ggacggcttc atgtccgccg
     5641 tccacctctg ccgcgacctg ctcgggcggg agccgtggtg agagatgggc atggcctgac
     5701 ttttgccgaa aaagggtgcg tcgccagaat gatgcaggcc gggagtggcg cagcccgccc
     5761 gacttttggg aggcgtcgtt tcgcatcgtg cgcccaccag acgaccgggc taaagccaca
     5821 gtcgtcctac gctacatcat tccggcaacg tgaatagttg aatagttgcc aaaatagtta
     5881 aatagtttaa atagttgcca ggcgttttgt acagtccgct gtgaatcaat agaaacggtg
     5941 gactgatcgg agcgaagaaa agccgcgcag cgatgcgtaa aggtggcttt ctggtgctca
     6001 cccggcgtgt ggacggtgcc gggcggctgg cggcggtgga gtggtccttc gcgggggtgg
     6061 agcgcgtggt gcggtatgat tatgactcgc attcccggct ggagcaggct agtttcggcc
     6121 tcatgaacca gccgccccag gtcagcgccg gctaccactg gctgcccggc acccgccgtg
     6181 tggagcggct ggagcgcccc atcgcgggca gcgcccagcc tttgaccggc gtcttcaccc
     6241 atgacgcggc cgggcgtctg gaaacgctgg agtggcggcg cggcggcacg ctttacggca
     6301 gccatgagta cggcttcgac gccgccggac gccgccagac cgagacccgg caggacggcg
     6361 ccaccctgca ctacgcctac acgccgcgcg gggagctg
//
LOCUS       DAOMYJ010000265         6381 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Prosthecobacter sp. isolate AMR_MDS_5629
            NODE_4149_length_6381_cov_21.288018, whole genome shotgun sequence.
ACCESSION   DAOMYJ010000265 DAOMYJ010000000
VERSION     DAOMYJ010000265.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867850
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prosthecobacter sp. (wastewater metagenome)
  ORGANISM  Prosthecobacter sp.
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Verrucomicrobiaceae; Prosthecobacter.
REFERENCE   1  (bases 1 to 6381)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6381)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.30
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:34:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,742
            CDSs (total)                      :: 4,684
            Genes (coding)                    :: 4,676
            CDSs (with protein)               :: 4,676
            Genes (RNA)                       :: 58
            tRNAs                             :: 55
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6381
                     /organism="Prosthecobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4149_length_6381_cov_21.288018"
                     /isolate="AMR_MDS_5629"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1965333"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1216)
                     /locus_tag="PLP58_19590"
     CDS             complement(<1..1216)
                     /locus_tag="PLP58_19590"
                     /inference="COORDINATES: protein motif:HMM:NF019222.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1552 domain-containing protein"
                     /protein_id="HRJ10793.1"
                     /translation="MSVPGSMSMRQLPQTRRHFLKSSGVTLALPLMESLGWRAFAATS
                     SARPQRMVCIGNALGFYQPAFWPEKTGRGYDLPELLQPLAAHQKDFTLFQGLDHGHKG
                     GHFAIHSYLSGVRTMDAKGMPDGNITVDQRAAESIGGATRFPSLAIGSEDGIHGGCLM
                     SWTRSGTRVPPIPGPRELFHKLFVSDNAADLARTQDKLALHGSILDAVHGDARDLKKT
                     LGRRDQEKLDEYFTSVRDVERQLELSRQWSAVPKPQPGMAEPENTGFVSDLPVLYDLI
                     ALALQTDSTRIATLEIAGGFEAAAFDLRKDYHALSHHGQVQENIDGLLKLERYQTGQF
                     ARFLGKMKSIEDGEGTLLDHSMVLFGSGMANANAHTNVNLPIILAGGGFKHGEHKAYP
                     TSGLNKQPLCNLY"
     gene            complement(1270..2070)
                     /locus_tag="PLP58_19595"
     CDS             complement(1270..2070)
                     /locus_tag="PLP58_19595"
                     /inference="COORDINATES: protein motif:HMM:NF015000.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HRJ10794.1"
                     /translation="MNVIPTAAFAAFIVMTAISLHAVEIVAHRGFSERAPENTVAAFK
                     LAWEHGADACELDLHLTADGEIAAIHDKDTKRTTGVLKVVKDSTLAELRALDAGSWKD
                     ARHAGERIPSLAECLATMPEGRGRFFLEIKCGPEVVPVLARQLEAWKPRAAQLCIIAF
                     DREVARESKKALPWLKVFRLSSEVTKDKRPVDLAALIADTKADGLDGLDLGLKWNWNE
                     EMARQIRAAELELYVWTVNKPEDARRLAALGVDGITTDDPVMLREALK"
     gene            complement(2122..3780)
                     /locus_tag="PLP58_19600"
     CDS             complement(2122..3780)
                     /locus_tag="PLP58_19600"
                     /inference="COORDINATES: protein motif:HMM:NF015094.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AarF/ABC1/UbiB kinase family protein"
                     /protein_id="HRJ10795.1"
                     /translation="MEPLARLERHAKRLGEIAAVAGKYGLSDWFGGFGYPWLQDRLRS
                     SDGQTLSGLTTAARLRLALTELGTTFIKLGQMLSTRADIVGAELAGELAGLQSNVPAD
                     LSGAARETLEADLGGKVEELFKSFDDTPLAAASIAQVHGARLPGGESVVVKIRRAGIE
                     AKVVTDLEIVQGLAELLEKHSPGLRPYQPVAITRQFRRTLLREMDFSYEKRNLQDFAR
                     RFERDATVHIPRVFEELCSHQIITMERLEGIPGTDVAALQASGEDLAEFARRGANMYL
                     EMVFRDGFYHADPHPGNLMLLPGCVVGVLDCGQVGRLDDELRDEVEALLLAVVENDAD
                     QVTESVLRLGAVPPDCARDRLRGDLDDFMSDYIGHPLGDIHVGQALSNLIEIIRSHHI
                     TLPPPLALLLKTLILLEGTSRRFNPGFSLAELMAPFCRRMALRRLSPRRLQRRARRFI
                     RDWDRLVTTLPRDVADLLARFRDGTLTVHLDHRHLDPIVNRLVLGVLTAAIFLGSSEL
                     WSRQAPPLLWGVSVFGALGYLTSVWLGWRLLRAIRKSGNIDSRD"
     gene            complement(4009..5562)
                     /locus_tag="PLP58_19605"
     CDS             complement(4009..5562)
                     /locus_tag="PLP58_19605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009962059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase"
                     /protein_id="HRJ10796.1"
                     /translation="MKRLILLLALAAALGTARADRPNILFIFCDDLTTQAISAYGHKL
                     KLLETPHMDRIAKEGMTFQRCLVPNSICGPSRAVIQTGKYNHINGFYHNGSRFNGDQP
                     TMPKMLRAGGYQTAVIGKWHLVSDPQGFDHWHILPGQGAYYNPPMIRDGQQVKHEGYT
                     TDLIGDFSVEWLKKRDKTRPFLLMSQHKAPHREWAPALRHLGWHGGRQFPEPETLFDD
                     YTGRGKAEREQDMTLEKTFTLLDAKLTPPPSLNAEQLKVWNAYYEPLNEKYRQAGLSG
                     DDIVRWRYQRYMHDYLGTVKAVDENVGKLLDYLDEEGIAENTLVILSSDQGFYLGEHG
                     WFDKRWIYEESLTTPCLARWPGVVQPGSTCQPMVSIIDFPETFLEAAGLPVPADMQGR
                     SLMPLLKGSTPADWRTSFYYHYYEFPGPHSVRKHYGVVTDSYKLFHFYEPEVNYWSLI
                     DRQSDTHEMTNVYDKPEYAAARAELHAELTRLRAELKVPAEDPPEAYNRPGSGKAKGL
                     MKKKSPGQK"
     gene            complement(5668..>6381)
                     /locus_tag="PLP58_19610"
     CDS             complement(5668..>6381)
                     /locus_tag="PLP58_19610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ10797.1"
                     /translation="VGGVGSGHISADYYGMYCAALEKRHTRADDGPPFVAMMANGTSG
                     DVNNVNFRQPRPRKGPYEQMRHVAEDVAAKVHGALSGLEWSNAASLAARYRELDVKWR
                     TIEPELLAWAKETEAAAPRIQGKTNLPLAYAGRVQHLAKASPQTRFPAQVLRIGDICI
                     GSSPCETFAETGLEFKKRSPFPKSFMVELAHGYYGYMPTPRHFPLGGYETWPGTNNVE
                     PQASVKLMDALLEMAAELK"
BASE COUNT         1057 a         2047 c         2106 g         1171 t
ORIGIN      
        1 catagagatt gcacaggggc tgcttgttca gcccgctggt tggatacgcc ttgtgctcgc
       61 cgtgcttgaa gccgccgccg gcgaggatga tcgggaggtt gacgttggtg tgggcgttgg
      121 cgttggccat gccgctgcca aagaggacca tgctgtggtc gagcagcgtg ccttcgccat
      181 cctcgatgga cttcatcttg ccgagaaagc gcgcgaactg cccggtctga tagcgttcca
      241 gcttgagcag gccgtcaatg ttttcctgaa cctggccgtg atgtgagagg gcatggtaat
      301 ccttgcgcag gtcaaatgcc gcagcctcaa agcctccggc gatctccagc gtggcgatgc
      361 gggtggagtc ggtttggagc gcgagcgcga tgaggtcgta gagcacgggc agatcggaga
      421 caaagccggt gttttccggc tcggccatgc cgggctgcgg cttcggcacg gcggaccact
      481 ggcggctgag ttcgagctgc ctctccacat cgcgcacgga ggtgaagtat tcgtcgagct
      541 tctcctggtc gcgcctgccg agggtctttt tgaggtccct ggcatcgcca tgcacggcgt
      601 cgaggatgct gccatgcaga gccagcttgt cctgagtgcg ggccagatcg gcggcgttgt
      661 cggagacgaa gagcttgtgg aaaagctcgc gcggaccggg gatcggcggc acgcgggtgc
      721 cgctgcgcgt ccaggacatg aggcagccgc cgtggatgcc gtcctccgag ccaatcgcaa
      781 gcgagggaaa gcgcgtggcc ccgccgatgc tttccgccgc gcgctggtcc acagtgatgt
      841 tgccatccgg catgcctttg gcgtccatcg tgcgcactcc gctgaggtag gagtggatgg
      901 cgaaatgccc gcctttgtgt ccatggtcga gcccctggaa aagcgtgaag tctttttgat
      961 gcgcggcgag cggctgcagc agctccggca ggtcatagcc gcgcccggtt ttctccggcc
     1021 agaacgcagg ctggtaaaag ccgagcgcat tgccgatgca gaccatgcgc tgcggacgcg
     1081 cgctggacgt ggcggcaaag gcgcgccagc ccagcgactc catcaatggc agcgcgagag
     1141 tgacgccgga ggatttgagg aaatggcggc gggtttgagg caattgacgc atggacatgg
     1201 agccgggaac ggacatgggc cgcgcggctt tcattcagct tcgcgaatcc acgcgctcca
     1261 gtcgccgctt catttcaaag cctcccgcag catcacggga tcatctgtcg tgatgccatc
     1321 cacgccaagc gcggccaggc ggcgcgcatc ctccggcttg ttcaccgtcc acacatacag
     1381 ctccagctcc gccgcgcgga tctggcgcgc catctcctcg ttccagttcc atttcagccc
     1441 caggtccagg ccatccagcc cgtccgcttt cgtgtccgcg atcagcgccg ccaggtccac
     1501 cggcctcttg tccttcgtca cctccgacga aagccggaac actttcagcc agggcagagc
     1561 ctttttcgac tcccgcgcca cctcgcggtc aaaagcgatg atgcagagtt gcgcggcgcg
     1621 cggcttccat gcctcaagct ggcgcgccag caccggcacc acttcagggc cgcatttgat
     1681 ctccaggaaa aagcgccccc tgccctcagg catcgtcgcc agacactccg ccagagatgg
     1741 aatgcgctcc ccggcgtggc gcgcgtcctt ccacgacccc gcatccagcg cgcgcagctc
     1801 cgccagagtc gagtccttca ccaccttgag cacacccgtc gtgcgtttcg tgtccttgtc
     1861 atgaatcgcc gcgatctccc cgtccgccgt caggtgcaga tccagttcgc aggcgtccgc
     1921 gccgtgttcc caggccagtt tgaatgccgc caccgtgttc tcaggcgccc gctccgagaa
     1981 gccgcggtgc gccacaatct cgacggcatg cagggaaatg gcggtcatga caataaaggc
     2041 ggcaaaagcg gcggtaggga tgacattcat caacgcaaac aacgcgcctc cacaccgcca
     2101 gcttgccgcg catctcgccg gctaatcccg cgagtcaatg ttccccgact tgcggatcgc
     2161 gcgcagcagc cgccagccca gccacacgct ggtcagataa cccagcgcgc cgaacaccga
     2221 cacaccccac agcagcggcg gcgcctgccg gctccacagc tcgctcgaac ccagaaaaat
     2281 ggccgccgtc agcaccccca gcaccagccg gttcacgatc gggtccagat gccggtggtc
     2341 cagatgcaca gtcagcgtgc cgtcccggaa ccgcgccagc aggtccgcca catcccgcgg
     2401 cagcgtcgtc accagccggt cccagtcccg gatgaagcgg cgcgcgcggc gctgcagccg
     2461 gcgcggcgag agcctgcgca gcgccatgcg ccggcaaaac ggcgccatca gctccgccag
     2521 gctgaacccc ggattgaagc gccggctcgt cccctccagc aggatcagcg tcttgagcag
     2581 cagcgccagc ggcggcggca gcgtgatgtg gtggctgcgg atgatctcga tcagattgct
     2641 cagcgcctgc cccacatgga tatcccccag cggatggccg atgtaatccg acatgaaatc
     2701 atccaggtcg ccccgcagcc ggtcccgcgc gcagtccgga ggcaccgccc ccagccgcag
     2761 caccgactcc gtcacctggt ccgcgtcatt ctccaccacc gccagcagca gcgcctccac
     2821 ctcgtcccgc agctcgtcat ccagccggcc cacctgcccg cagtccagca cacccaccac
     2881 gcaacccggc agcagcatga gattccccgg atgcgggtcc gcgtggtaga agccgtcgcg
     2941 gaacaccatc tccagataca tgttcgcccc ccggcgggcg aactccgcca gatcctcccc
     3001 ggacgcctgc aaagccgcca catccgtgcc cggtatcccc tccagccgct ccatcgtgat
     3061 gatctggtgc gagcacagct cctcaaacac gcgcggaata tgcaccgtcg catccctctc
     3121 aaagcggcgc gcaaagtcct gcaaattccg cttctcatag ctgaaatcca tctcccgcag
     3181 cagcgtccgg cggaactgcc gtgtgatcgc caccggctga tacggacgca gccccggcga
     3241 gtgcttctcc agcaactccg ccagcccctg cacgatctcc aggtccgtca ccaccttcgc
     3301 ctcgatgccc gcccggcgga tcttcaccac aacactctcc cctccgggca ggcgcgcccc
     3361 atgcacctgc gcgatggacg ccgccgccag aggcgtgtca tcaaagctct tgaaaagctc
     3421 ctccaccttg ccgcccaggt ccgcctccag agtctcccgc gccgccccgc tcaaatccgc
     3481 cggcacattc gattgcagcc ccgccaattc ccccgccaac tccgctccca caatgtccgc
     3541 gcgcgtgctc agcatctggc ccagcttgat gaatgtcgtc cccagctccg tcagcgccag
     3601 gcgcaaccgc gccgccgttg tcaggcccga aagcgtctgc ccgtcggaac tgcgcagccg
     3661 gtcctgcagc cacggatagc cgaagcctcc gaaccaatcc gaaagcccgt acttgcccgc
     3721 cacggcggcg atttccccca ggcgcttggc atggcgttcc agacgtgcga gcggttccac
     3781 tttcatatcg cccactctgc tcccgcccgc cgccttcaca acccggcttt tcaaagtccc
     3841 gtgtttgcag tcccgccgcc gggatacggg gcttccgaac ccggctttgg cacacgcgcc
     3901 cgcagatcca gccgcagagg tttgatcgcc tccccgatga tctcaaactt ggcctcagag
     3961 gccgtccaca ccatgccgca taggttcgca gggagggcgg cggcagcgtt atttttggcc
     4021 ggggcttttc ttcttcatca ggcctttggc tttgccgctg ccggggcggt tgtaggcttc
     4081 aggcgggtct tcggcgggga ctttgagttc ggcgcgcagg cgggtgagct cggcgtggag
     4141 ttcggcgcgg gcggcggcgt actcgggttt gtcatagacg ttggtcatct catgggtgtc
     4201 gctctggcgg tctatgaggg accagtagtt gacctccggt tcgtagaagt ggaagagctt
     4261 gtagctgtcg gtgacgacgc cgtagtgctt gcgcacgctg tgcgggccgg ggaactcata
     4321 gtagtggtag taaaaagagg tgcgccagtc ggcgggtgtg ctgcctttga gcaggggcat
     4381 gagggagcgg ccctgcatgt cggcggggac gggaaggccg gcggcttcga ggaaggtctc
     4441 ggggaaatca atgatggaga ccatgggctg gcaggtgctg ccgggttgga cgacgccggg
     4501 ccagcgggcg aggcagggag tggtgaggga ttcctcatag atccagcgtt tgtcgaacca
     4561 gccgtgctcg ccgaggtaga agccctggtc ggaggagagg atgacgaggg tgttttcagc
     4621 gatgccttcc tcgtcgaggt agtcgaggag tttgccgacg ttttcgtcca cggctttgac
     4681 ggtgccgagg tagtcgtgca tgtagcgctg gtagcgccag cggacgatgt cgtcgccgct
     4741 gaggccggcc tggcggtatt tttcgttgag gggctcataa taggcgttcc agaccttgag
     4801 ctgctcggcg ttgaggctgg gcggcggggt gagcttggcg tcgaggaggg tgaaggtttt
     4861 ttcgagagtc atgtcctgct cgcgctcggc tttgccgcgg ccggtgtagt cgtcgaagag
     4921 ggtctcgggc tcggggaact ggcggccgcc atgccatccg aggtggcgga gcgctggcgc
     4981 ccactcgcgg tggggcgcct tgtgctggct catgaggaga aagggcctgg ttttgtcgcg
     5041 cttcttgagc cactcgacgg agaaatcgcc gatgaggtcg gtggtgtagc cctcgtgttt
     5101 gacctgctgg ccgtcgcgga tcatgggggg attgtaataa gcgccctggc cggggaggat
     5161 gtgccagtgg tcgaagccct gggggtcgct gacgagatgc catttgccga tgacggcggt
     5221 ctggtagccg ccggcgcgga gcattttggg catggtgggc tggtcgccgt tgaaacggct
     5281 gccgttgtgg tagaagccgt tgatgtggtt gtatttgccg gtctggatga cggcgcggct
     5341 ggggccgcag atggaattgg gcacgaggca gcgctggaag gtcatgccct ctttggcgat
     5401 gcggtccatg tgcggggtct caagcagctt gagcttgtgc ccgtaggcgc tgatggcctg
     5461 ggtggtcaag tcgtcgcaga agatgaaaag gatgttgggc cggtcggcgc gcgcggtgcc
     5521 gagggctgcg gccagagcaa ggaggaggat gaggcgtttc atggagcggc gtgggaacgc
     5581 gcgggccgcc ggcatcttgc gggtgaacac agccgctgtt gaattcggag gcggggcact
     5641 tggcatgcgg ctctccttgg ggacgggtca cttcaattcg gcggccattt ccaggagggc
     5701 gtccatgagt ttcacgctgg cttgcggctc gacgttgttg gtgccgggcc aggtctcgta
     5761 acctccaagc gggaagtggc gcggcgttgg catgtagccg tagtaaccgt gggcgagttc
     5821 gaccatgaac gacttgggaa agggactgcg ctttttgaat tcgaggccgg tctcggcaaa
     5881 ggtctcgcag ggggaggagc cgatgcagat gtccccaatg cgcaggacct gggcgggaaa
     5941 acgggtctgc gggctggctt tggcaaggtg ctgcacgcga ccggcgtagg cgaggggcag
     6001 gttcgtcttg ccctggatgc gcggcgcggc ggcctcggtt tctttggccc aggcgagcag
     6061 ttcgggctcg atggtccgcc atttcacatc cagctcgcgg tagcgcgcgg ccagggacgc
     6121 ggcgttgctc cattcgaggc cggagagcgc gccatgcact ttggcggcca catcttcggc
     6181 gacatggcgc atttgctcat acggtccctt gcgcggacgg ggctggcgga agttgacatt
     6241 gttgacgtcg ccgctggtgc cgttggccat catggcgaca aaaggcgggc cgtcgtccgc
     6301 gcgcgtatgc cgcttttcca aggcggcgca atacatgccg taataatcgg cggagatgtg
     6361 gccggagccg acgccgccga c
//
LOCUS       DAOMYJ010000266         6201 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Prosthecobacter sp. isolate AMR_MDS_5629
            NODE_4297_length_6201_cov_24.490075, whole genome shotgun sequence.
ACCESSION   DAOMYJ010000266 DAOMYJ010000000
VERSION     DAOMYJ010000266.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867850
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prosthecobacter sp. (wastewater metagenome)
  ORGANISM  Prosthecobacter sp.
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Verrucomicrobiaceae; Prosthecobacter.
REFERENCE   1  (bases 1 to 6201)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6201)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.30
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:34:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,742
            CDSs (total)                      :: 4,684
            Genes (coding)                    :: 4,676
            CDSs (with protein)               :: 4,676
            Genes (RNA)                       :: 58
            tRNAs                             :: 55
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6201
                     /organism="Prosthecobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4297_length_6201_cov_24.490075"
                     /isolate="AMR_MDS_5629"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1965333"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..353)
                     /locus_tag="PLP58_19615"
     CDS             complement(<1..353)
                     /locus_tag="PLP58_19615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009959620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoglycerate dehydrogenase"
                     /protein_id="HRJ10798.1"
                     /translation="MRVLLTTTSYQDTPGEHHALLESQGWHIHRERGPLPEARMLELA
                     GEFDAFLCGDDEITKAVLDKALPRLRVISKYGIGLDKIDVEECTARRLPVLFTPGVNH
                     TTVAEHTFCLLLALVR"
     gene            528..3023
                     /locus_tag="PLP58_19620"
     CDS             528..3023
                     /locus_tag="PLP58_19620"
                     /inference="COORDINATES: protein motif:HMM:NF019223.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PSD1 and planctomycete cytochrome C
                     domain-containing protein"
                     /protein_id="HRJ10799.1"
                     /translation="MRLHFFAAAFPAFALSAETAPEGHASIPADQLNFFEKNIRPVLV
                     EHCHQCHSAESDKVKGGFTLDTKQGLELGGESGHPGVTPGRPDQSTLYEAVTWENPDM
                     QMPPKNKLPADVIANIKKWIEMGAPDPRVQEVPNAAGGRRVIDMDEGRRHWAFQKPVK
                     PQPPEVKKADWAKTDIDRFILAGLEEKGLTPMPDADRRTLIRRIAYDLTGLPPTPDEV
                     RAFVEDKSPEALKRVIDLYLDSPRFGERWARHWLDVARYAESSGKEINILYPHAWRFR
                     DYVIDAFNRDKPYDQFLKEQIAGDLLGFENKRDQAEKIVATGFLAIGAKGHNTRDRRQ
                     FMMDLVDEQIDAVSQSMLGLTLACARCHDHKFDPVTQRDYYALAGIFLSSEPLYGTYS
                     QLQNLTPSTLIELDLEAGQIASLASISAREVQELKNRVSQAAEAAEQVQREVLSERDA
                     GNPAARFLRVRAARDRASTIQADLDLFHEDGTPRALAMGVLDRERPANTPLLVRGDIK
                     QPSDIVPRGLVEVLCEKGEPLSIGEGSGRLDLAYWIASKDNPLTARVMANRVWLKLMG
                     QGIVPTPDNFGIMGFAPTHPELLDYLAVTFMEKDWSVKQLIREILMSRVYQLGSGHHA
                     QNYAVDPDNTWRWRMTPRRLEAEAIRDAMLAVSGELNLYPVDGSPVARAGEGREGLIN
                     LAREVASKPFNYRSIYLPIVRDQIPEFLNVFDFPDASLVSGERDSTNVPGQSLFLMNN
                     PQVLALADAFARRIAEYEGDGPQRLAYAYELVFARPPTEAEMTAIKRFWQEFPRQAAG
                     GKNTAQAQEQARKAALSAFCQGLFASAEFRYIN"
     gene            3058..4464
                     /locus_tag="PLP58_19625"
     CDS             3058..4464
                     /locus_tag="PLP58_19625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018969253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1501 domain-containing protein"
                     /protein_id="HRJ10800.1"
                     /translation="MNTRRDFLKTTSNGFGWLAFSALAAQQGLRASSVLAPKSPHHAA
                     RAKRVIFLCMQGGPSHVDLFDYKPALAGASGKSAPSAAGRYGRANLMASPWKFQQHGR
                     SGLWISDLLPNLAKHADDLCLLNSMTTDLPAHPQAFTQLHTGSTQFVRPSLGAWTLYG
                     LGTQNENLPGFVTINPPANATRTYGSAFLPAIYQGAKVGGSTVPGIPPQLAARLGRGA
                     QQATIANIKNERFGTGAQRAQLDLVQSLNRARMAQDGGVNSEVEGVIESYELAFRMQA
                     EVPKVLDLESESAATKTLYGIGAGETDNFGRQCLMARKLVEAGVRFVEISLGNWDHHF
                     NLTTAMERMTGQMDKPIAGLLQDLKQRGLLKDTLVIWGGEFGRTPHSQGDDGRDHNNK
                     GFTMWMAGGGVKGGMAYGSTDELGYEAVLDKMHIHDWHATVLHLLGLDHEKLTYRYAG
                     RDFRLTDVHGVVAKEILA"
     gene            complement(4477..5526)
                     /gene="hemA"
                     /locus_tag="PLP58_19630"
     CDS             complement(4477..5526)
                     /gene="hemA"
                     /locus_tag="PLP58_19630"
                     /EC_number="1.2.1.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009964498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008883 - glutamyl-tRNA reductase activity
                     [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl-tRNA reductase"
                     /protein_id="HRJ10801.1"
                     /translation="MPQATEKSGRLVCLGLNYRTTPVEVREKVAFPQPAVPAALREVA
                     ALPGFDESVVLSTCNRVELYAAHHLDDCAQAHATLRDYLVRRFDLATGQAEALVTYGL
                     DSAEAARHLFRVVSGLDSMVLGETEIFGQVKDAYKAALETGVTGRALNKLFQHAFSTG
                     KKVRNQTSIQRGSTSVGSVAVDLAEKIHDLAECRVMLVGAGEMSRTCAQSLLSRGAKS
                     IIVSNRNYDRAVELAAEMRGSAIKFDAWEASMHEVDVIISSTSAPHFVIHPEQIRQVM
                     RKRRWEPLLIIDIAVPRDVDPRVNEIEGVYLYDIDALQAIADEGRRERQRQLAACEQI
                     IEQQLEKFGLAASRE"
     gene            complement(5530..>6201)
                     /gene="ccsA"
                     /locus_tag="PLP58_19635"
     CDS             complement(5530..>6201)
                     /gene="ccsA"
                     /locus_tag="PLP58_19635"
                     /inference="COORDINATES: protein motif:HMM:NF013725.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcsA"
                     /protein_id="HRJ10802.1"
                     /translation="VQLAAMLAGFALQTASIYLRGQQVGQCPMKSLPDILVFVAWSIA
                     LLYFLVGSTFRVSLLGMFTAPLLVVMHGLAFSFPGGFRPYPVKGQIIALVELHAALAL
                     VAYAAFALACVTGVMYLLQERLLKKHLIGGLFYELPPIQGLARAIQRMVMLGVVLLSA
                     SLGISFKLEGVAAGPKLAIAWGVWALYTLISIVIWRHTLSPRQTAWLAALGFVIPFIS
                     LWLVT"
BASE COUNT         1121 a         2040 c         2035 g         1005 t
ORIGIN      
        1 cgcaccagcg ccagcagcag gcagaaggtg tgctccgcca cggtggtgtg gttcacgccc
       61 ggcgtgaaga gcaccggcag ccgccgcgcg gtgcattcct ccacgtctat cttgtccagg
      121 ccgatcccgt atttgctgat gacccgcagc cggggcagcg ccttgtccag caccgccttt
      181 gtgatctcgt catcgccgca gagaaaggcg tcgaactccc cagccagctc cagcatccgc
      241 gcctccggca gcgggccgcg ctcgcggtgg atgtgccagc cctgggactc gagcagggca
      301 tgatgttcgc cgggggtgtc ttgatagctg gtggtggtga gcaggacgcg catggctctc
      361 caggatgggc ggctgccgct ccccggcaag ccaagtttga ccctggcgga cgatgagact
      421 ttcccgcgcg gcgctgccag gcggaacggg cattcgcgcg ggagcagcgt tcgggcacgc
      481 gcaactttca tccgcagggc gggtttcctg ccggcacaag caccgccatg agactccact
      541 tttttgccgc cgcctttccc gccttcgcgc tgtccgcgga gaccgcgcct gagggtcacg
      601 cctccatccc ggcggaccag ctcaacttct ttgaaaagaa catccgcccc gtgctggtgg
      661 agcactgcca ccagtgccac tccgccgagt cggacaaggt gaagggcggc ttcacgctcg
      721 acacgaagca gggcctcgaa ctcggcggcg agtcgggcca tcccggtgtc acccccggcc
      781 ggccggacca gagcacgctg tatgaggcgg tgacctggga gaacccggac atgcagatgc
      841 cgccaaagaa caagctgccg gcggatgtga tcgccaacat caaaaagtgg atcgagatgg
      901 gcgcgccgga cccgcgcgtg caggaggtgc caaacgcggc gggcggacgc cgggtgattg
      961 acatggacga ggggcgcagg cactgggcct tccaaaagcc ggtgaagccg cagccgccgg
     1021 aggtcaagaa ggcggactgg gcgaagacgg acattgaccg cttcatcctc gccggcctgg
     1081 aggagaaggg cctgacgccc atgcccgacg cggaccggcg cacgctcatc cgccgcatcg
     1141 cctacgatct caccggcctg ccgccgacgc cggacgaggt gcgcgccttt gtcgaggaca
     1201 aatcgccgga ggcgctgaag cgtgtcattg acctgtatct ggactcgccc cgcttcggcg
     1261 agcgctgggc gcggcactgg ctggatgtgg cgcgctacgc ggagagcagc ggcaaggaga
     1321 tcaacatcct gtatccccac gcctggcgtt tccgcgacta tgtgattgac gccttcaacc
     1381 gcgacaagcc ctacgaccag tttctcaaag agcagatcgc cggggatttg ctgggcttcg
     1441 agaacaaacg ggaccaggcg gaaaagatcg tggccacggg tttcctcgcc atcggcgcga
     1501 aggggcacaa cacgcgcgac aggcggcagt tcatgatgga cctcgtggac gagcagatcg
     1561 acgccgtgtc gcagtccatg ctcggcctca cgctggcctg cgcgcgctgc catgaccaca
     1621 agttcgatcc cgtgacgcag cgcgactatt acgcgctggc gggcatcttc ctgagcagcg
     1681 agccgcttta cggcacctac agccagcttc aaaacctcac accctccacg ctcatcgaac
     1741 tcgacctcga ggcagggcag atcgcctcgc tggcatccat ctccgcgagg gaggtgcagg
     1801 agctgaaaaa ccgcgtcagc caggccgccg aggccgccga gcaggtgcag cgcgaggtgc
     1861 tctccgagcg tgacgcgggc aacccggcgg caaggttcct ccgcgtccgc gccgcccgcg
     1921 accgcgcctc caccatccag gcggacctgg accttttcca tgaggacggc acgccgcgcg
     1981 cgctggcgat gggcgtgctg gaccgcgagc gtcctgccaa caccccgctg ctcgtgcgcg
     2041 gcgacatcaa gcagccctcc gacatcgtgc cgcgcggcct ggtggaggtg ctctgcgaga
     2101 aaggcgagcc gctcagcatc ggcgaaggca gcggcaggct ggacctggcg tattggatcg
     2161 cctccaagga caacccgctc accgcgcgcg tgatggcgaa ccgtgtctgg ctgaagctca
     2221 tggggcaggg catcgtgccc acgccggaca acttcggcat catgggcttc gcgccgacgc
     2281 atcctgaact cctggattat ttggccgtga ctttcatgga aaaggactgg tccgtgaagc
     2341 agctcatccg cgagattctc atgagccgcg tctatcagct cggctccggt catcatgcgc
     2401 aaaactacgc ggtggaccct gacaacacct ggcgctggcg catgaccccg cgccgtctgg
     2461 aagccgaggc catccgggac gccatgctgg ccgtctccgg cgagctgaat ctctatcccg
     2521 tggacggctc acccgtggcg cgtgctggag aggggcgcga gggcttgatc aatctggcgc
     2581 gcgaggtggc atcaaagccc ttcaactacc gcagcatcta cctgcccatc gtgcgcgacc
     2641 agattcccga gttcctcaac gtcttcgact ttcccgatgc gagtctggtg agcggagagc
     2701 gcgactcgac gaacgtgccg ggccagagcc tcttcctcat gaacaacccg caggtgctcg
     2761 ccctggcgga cgccttcgcg cggcgcatcg ccgaatacga gggcgacggg ccgcagcgtc
     2821 tcgcttatgc ctatgagctg gtctttgccc ggccgccgac cgaggctgag atgaccgcca
     2881 tcaagcgctt ctggcaggaa ttcccccgcc aggccgccgg cggcaaaaac accgcgcaag
     2941 cccaggagca ggcccgcaag gccgccctct cagccttctg ccaggggttg ttcgcgagcg
     3001 cggagtttcg ctacatcaac tgattttctt ccatcccaac tcgaacactg aactgccatg
     3061 aacacgcgtc gcgacttcct caaaaccacc tcgaacggct tcggctggct ggccttttcc
     3121 gcgctggccg cccagcaagg cttgcgcgcc agctcggtgc tggcgccgaa gagcccgcac
     3181 catgccgcgc gggcgaagcg ggtcatcttt ctctgcatgc agggtggtcc ttcgcatgtg
     3241 gacctctttg attacaaacc cgcgctggcc ggcgcctcgg gcaagagcgc gccttccgcg
     3301 gcgggacgtt acgggcgcgc gaacctgatg gcctcgccct ggaagttcca gcagcatgga
     3361 cgcagcggtc tgtggatttc cgatctgctg cccaacctgg ccaagcacgc ggatgatctc
     3421 tgcctgctga actcgatgac caccgacctg cccgcgcatc cgcaggcctt cacgcaactg
     3481 cacacgggca gcacgcagtt tgtccggccc tcgctcgggg cgtggacgct ttacgggctg
     3541 ggcacacaga atgaaaacct gcccggcttt gtcaccatca acccgcccgc gaatgccacg
     3601 cgcacttacg gcagcgcgtt cctgccggcg atttaccagg gagccaaggt gggtggcagc
     3661 accgtgcctg gaatcccccc gcagctcgcc gcgcgcctgg ggcgcggcgc gcagcaggcg
     3721 acgatcgcca acatcaaaaa cgagcgtttt ggcacgggcg cccagcgcgc gcagctcgac
     3781 ttggtgcagt cgctcaaccg cgcgcgcatg gcgcaggatg gcggcgtgaa ctccgaggtg
     3841 gagggcgtca tcgagtccta cgagctggcc ttccgcatgc aggccgaggt gccgaaggtg
     3901 ctggatctgg agagcgagag cgcggccacc aagacgctat acggcatcgg cgcgggcgag
     3961 acggacaact tcggccgcca gtgcctcatg gcgcgcaagc tggtggaggc gggcgtgcgt
     4021 tttgtcgaga tcagcctcgg caactgggat caccatttca atctcaccac cgccatggag
     4081 cgcatgaccg ggcagatgga caagccgatc gcgggtctcc tgcaggacct caagcagcgc
     4141 ggtcttctga aggacacgct ggtcatctgg ggcggcgagt tcggccgcac gccgcacagc
     4201 cagggcgacg acggacgcga ccacaacaac aaaggcttca cgatgtggat ggccggcggc
     4261 ggcgtgaagg gcggcatggc ttacggcagc acggacgagc tgggctacga ggcggtgctg
     4321 gacaagatgc acatccacga ctggcacgcc acggtgctgc atctgcttgg cctggatcac
     4381 gaaaaactca cttatcgcta cgccggccgc gacttccgcc tcacggacgt gcatggcgtg
     4441 gtggcaaaag agatcctggc gtgatctccc ggcgcatcat tcgcgcgatg ctgccaggcc
     4501 gaatttttca agctgctgct cgatgatctg ctcgcaggcg gcgagctggc gctggcgctc
     4561 gcggcggccc tcgtcggcga tggcctggag ggcgtctatg tcatagagat agacgccctc
     4621 gatctcgttg acgcgcgggt ccacgtcgcg cggcacggcg atgtctatga tgagcagcgg
     4681 ctcccagcgg cgcttgcgca tgacctgccg gatctgctcc gggtggatga cgaaatgcgg
     4741 cgcgctggtg ctggagatga tgacatccac ctcatgcatg gaggcctccc atgcgtcgaa
     4801 tttgatggcg ctgcccctca tctcggcggc cagctcgacg gcgcggtcat agttgcggtt
     4861 ggagacgatg atgctcttcg ctccgcgcga gagcaggctc tgggcgcagg tgcggctcat
     4921 ctcgcccgcg ccgacgagca tgacgcggca ctcggccagg tcgtggattt tctccgccag
     4981 gtccaccgcc acgctgccga cactggtgga gccgcgctgg atgctggtct ggttgcggac
     5041 ttttttgccg gtggagaagg cgtgctggaa gagcttgttg agcgcgcggc cggtgacgcc
     5101 ggtttcgagc gcggctttgt aggcgtcttt gacctggccg aagatttccg tctcgccaag
     5161 caccatgctg tcgagcccgc tgacgacccg aaagagatgc cgggccgctt cggcggagtc
     5221 gagtccgtag gtcacgagcg cctcggcttg accggtggcg aggtcaaagc ggcgcacgag
     5281 gtagtcgcgc agagtcgcat gagcctgcgc gcagtcatcc aggtgatgcg cggcatacag
     5341 ctccacacgg ttgcaggtgc tgagcacgac gctttcatca aatcccggca aggccgccac
     5401 ctcgcgcagg gcggcgggca cggccggctg ggggaaggcc accttctcgc gcacctccac
     5461 cggcgtggtt cggtaattca atcccaggca gaccaggcgg ccgctttttt cagtcgcttg
     5521 aggcatgggt caggtgacaa gccagaggga gatgaagggg atgacgaagc ccagcgcggc
     5581 cagccaggcg gtctggcgcg gggatagcgt gtgacgccag atgacgatgc tgatgagcgt
     5641 gtaaagcgcc cacacacccc acgcgatggc gagttttggc cccgcggcga cgccttcgag
     5701 tttgaaactg atgcccaggc tcgcgctcag cagcaccacg cccagcatga ccatgcgctg
     5761 aatggcgcgg gccaggccct ggatgggcgg cagctcgtaa aacaggccgc cgatcaaatg
     5821 cttcttcaac agcctttcct gcagcaggta catcacccct gtgacgcagg ccagcgcgaa
     5881 agccgcgtag gccaccagcg ccagggcggc gtgcagctcc accagcgcga tgatttgtcc
     5941 tttcaccggg tagggacgga atccgccagg aaatgagaag gccagcccgt gcatcaccac
     6001 cagaagcggc gcggtgaaca tgcccagcag cgagacacgg aaagtggagc cgaccaggaa
     6061 ataaagcagc gcgatgctcc atgccacgaa gaccaggatg tcgggcaggc ttttcatagg
     6121 gcactggccc acctgctgtc cgcgcagata gatggaggcg gtctgcagcg cgaaccccgc
     6181 cagcatggcg gcgagctgca c
//
LOCUS       DAOMYJ010000267         6153 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Prosthecobacter sp. isolate AMR_MDS_5629
            NODE_4334_length_6153_cov_24.949328, whole genome shotgun sequence.
ACCESSION   DAOMYJ010000267 DAOMYJ010000000
VERSION     DAOMYJ010000267.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867850
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prosthecobacter sp. (wastewater metagenome)
  ORGANISM  Prosthecobacter sp.
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Verrucomicrobiaceae; Prosthecobacter.
REFERENCE   1  (bases 1 to 6153)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6153)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.30
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:34:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,742
            CDSs (total)                      :: 4,684
            Genes (coding)                    :: 4,676
            CDSs (with protein)               :: 4,676
            Genes (RNA)                       :: 58
            tRNAs                             :: 55
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6153
                     /organism="Prosthecobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4334_length_6153_cov_24.949328"
                     /isolate="AMR_MDS_5629"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1965333"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..240
                     /locus_tag="PLP58_19640"
     CDS             <1..240
                     /locus_tag="PLP58_19640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ10803.1"
                     /translation="PPPTHTPAVPGGVQTPPAPPPAPTSPAARSDVPFARRVPGQPNQ
                     VYSLKDPKRAISVEGFRPGQTVADPVTGDLFRVPY"
     gene            complement(268..1665)
                     /locus_tag="PLP58_19645"
     CDS             complement(268..1665)
                     /locus_tag="PLP58_19645"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HRJ10804.1"
                     /translation="MKATLFSLACLCLEAGAAAGRPDILFVVTDDQRPDTLAALHGAH
                     LRTPNLDRLAARGTVFTRAYAGYPICHVSRAEILTGCTAFRAYPNYPVGPVDPALKTL
                     AATFREAGYLAWYSGKWHNDGTPLQRGYTGIRGLYSSGGGGKKPAAPQTDERGNPLTG
                     YTGWTFKNDEGRPEPEKGAGLRPDNSRHIADGAIRALRETPAGQPFLLHVNFAFPHDP
                     RMWPPDPAHHHELHRTPLPANFAPEHPFDHGNQGGRDEVLLPVPRAEGAVRLELAVYH
                     SMITDLDAQLGRILAALEDTGRASNTIIVFTSDQGLALGSHGLLGKQNQYEHSIRSPL
                     IIAGPGLPAGRQNRALVMLRDLFPTFCDLASVPVPDTVQGRSLAPLLRGQVARVHEQV
                     FGVFTDTQRMICDERWKLILYPQAGRGQLFDLENDPHELRDLSANAMHSARMASLLGQ
                     LNAWRMSAGDPLAPH"
     gene            complement(1765..2718)
                     /gene="tal"
                     /locus_tag="PLP58_19650"
     CDS             complement(1765..2718)
                     /gene="tal"
                     /locus_tag="PLP58_19650"
                     /EC_number="2.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009959336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004801 - transaldolase activity [Evidence
                     IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase"
                     /protein_id="HRJ10805.1"
                     /translation="MNQLEQLKQHTIVVADTGDFEAMKAYKPRDATTNPSLILQASQK
                     PEYKSLVDQAVAEHKSSPLSGAAKIESIMDRILILFGVEILKIVPGRVSTEVDARLSF
                     DIQGNVDKARHLIAAYEKQGIPRERVLIKIASTWEGIKAAEILQKESINCNLTLLFSL
                     AQAVACAEGGIKLISPFVGRILDWFKKSTGQDYAGAEDPGVQSVTQIYNYYKHFGHST
                     EVMGASFRNKGEITELCGCDLLTISPALLGELQSAEESITPKLNAAHARSLQIERVPC
                     DEKSFRWLLNEDAMATEKTAEGIRNFAKDIVKLEKFIESCL"
     gene            2952..5000
                     /locus_tag="PLP58_19655"
     CDS             2952..5000
                     /locus_tag="PLP58_19655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009962952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HRJ10806.1"
                     /translation="MNRLALLTLALVMPVHAATEADVIVYGSTPGGFCAAIAAAREGA
                     SVILLEPTAHVGGVNTGGLCFSDSNQTVRSTVLGLFEEWHQCIENDYQQRGVELPYRV
                     SEKDHTHWTYEPHVAARVTKAMLDEAGVRVLARRVLMRAVKEGARLTRLVTNAGEFAA
                     KVFIDASYEGDLMAAAGVSWTIGREGRGEYGESLAGKQYPKARMAISGLDDSGRPLPL
                     ITTGDAGPEVEGDKNVMVYSFRLCVTRDPANRVPFPQPMNYDPARFELVRRYFAQEKR
                     PHILWDLYPLPGGKFDANNGIGKQFSMGLVGACNGWSEADEAGRAVIWEAHKQYTLEF
                     YHFLTTDPAVPEHLRKEMSELGLCRDEFPEYEHWSPQLYVREGRRMIGEHIVTQKDIV
                     EQPEKPDAIVVSSFPIDSHDCQRVGTGEFVVNEGTIMPVRMAGRRHGYPFHIPYRAIT
                     PKASECSNLLVPVALSCTHVGISSIRVEPTWMILGQSAGIAAALSAKRGIAVQELSYP
                     ILRERLLAQKQVLDLPVLPDLPPGPPMSVSIDPKSLPGIVVDDSEAALKGAWSRSSNF
                     KPHIGRGYLHDDRRADGLSEAVFRFRVPKAGNYDLRMAYSAHETRATRVPVIVETGGG
                     KTRLLVDQTLPLPLGRHFRGIGSAALDAETDVVITVTNQDTDGFVIVDALQVVEAGKD
                     "
     gene            5220..5834
                     /locus_tag="PLP58_19660"
     CDS             5220..5834
                     /locus_tag="PLP58_19660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ10807.1"
                     /translation="MHSTRSLLRLSAALAFLSMTGAALSQALPLDHPCAGLVKKYLQS
                     VVAQDWKTAANMLLPSSLERKQKETVAIIKTARTMTEEADMLERFGVKDIRDLEKLTP
                     QEFYITDRTAWHQRVNSSPEVIKRKQDTLKIDVLGLVEEKDKNYIHVTVRTSQETLTD
                     RIEELFLISFVKEDDKYLIWPEMKDRPIITPLNEAASKEEAKSK"
     gene            5886..>6153
                     /locus_tag="PLP58_19665"
     CDS             5886..>6153
                     /locus_tag="PLP58_19665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009962445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HRJ10808.1"
                     /translation="MLRDTIAAISTAHGGAALSVVRVSGPDALGVCGRVFRARRPLTE
                     LAPRTAFRACAADSSGRAFDEGLVLVFHGPASFTGEDLVEFHGHG"
BASE COUNT         1119 a         1829 c         2027 g         1178 t
ORIGIN      
        1 cccccgccca cgcacactcc ggcggttcca ggcggtgtgc agacaccccc cgcgccgccg
       61 ccagcgccga cttcacccgc cgcgcgctcg gatgtgccgt tcgcccgcag ggtgccggga
      121 cagccgaacc aagtctacag cctgaaggat cccaaacgcg ccatcagcgt ggaagggttc
      181 cgccccggtc aaacggtggc tgacccggtt acaggcgatc ttttccgcgt gccttattga
      241 ggcggtgccc cgccccgatt gcctggatca gtgtggcgcc agcggatcgc cggcagacat
      301 gcgccatgcg ttgagctggc ctagcaggct tgccattctc gcggagtgca tggcattggc
      361 tgagaggtcg cgcaattcat gcgggtcgtt ttccaagtcg aaaagctgcc cgcgccctgc
      421 ctgcggatag agaatcaatt tccagcgctc atcgcaaatc atgcgctggg tgtcggtgaa
      481 gacgccgaac acctgctcgt ggacacgcgc cacctgcccg cgcagcaatg gcgcgagact
      541 gcgcccctgc acggtgtccg gcacgggcac gctggcgaga tcgcagaagg tgggaaaaag
      601 atcgcgcagc atcactagcg cgcggttttg gcgtccggcg ggcaggcccg gtccggcgat
      661 gatgagcgga gagcggatgc tgtgctcgta ttggttttgc ttgccgagca agccgtggct
      721 gccgagcgcc agcccctggt cgctggtgaa gacaatgatc gtgtttgagg cgcgccccgt
      781 gtcctccaac gccgcgagaa tgcggccgag ctgcgcgtcg agatcagtga tcatggaatg
      841 atacaccgcc agctccagcc gcaccgcgcc ctcggcgcgc ggcacaggca ggagcacctc
      901 gtcgcgcccg ccctggttgc cgtgatcgaa gggatgctcc ggcgcgaagt tggcaggcag
      961 cggcgtgcga tgcagctcgt gatgatgcgc ggggtccggc ggccacatgc gcggatcgtg
     1021 gggaaaggcg aagttcacat gcagcaggaa gggctgcccg gcaggtgtct cccgcagagc
     1081 gcggatggcg ccgtcggcga tgtggcggct gttgtccggg cgcagccccg cgcctttctc
     1141 cggctccggt ctgccctcgt cattcttgaa ggtccagccg gtgtagcccg tgagcggatt
     1201 gccacgctcg tcggtctgcg gggccgccgg ttttttgccg ccgcctccgc tggagtagag
     1261 tccgcggatg cccgtgtaac cgcgctgcaa gggggtgccg tcattgtgcc atttgccgga
     1321 ataccaggcc agatagcccg cctcgcgaaa ggtggccgcg agtgttttca gcgcgggatc
     1381 aaccggcccg accgggtagt ttggatacgc gcggaaggcc gtgcagccgg tgaggatttc
     1441 cgcccggctg acatggcaga tggggtatcc ggcataagcg cgtgtgaaga ccgtgccccg
     1501 cgcggcgagc cggtcgagat tcggcgtgcg caaatgcgcg ccatgcagcg ccgcgagggt
     1561 gtccggcctc tggtcgtcgg tgacgacgaa caggatgtca ggccgcccgg cggcggcgcc
     1621 ggcttcaagg caaagacagg ccagggagaa aagcgtggct ttcatgcggt ggaggaatcc
     1681 cggtaatgcc ggccaatctt tccctcagcg cgcaaaaaag cgcggccccg tgaggagccg
     1741 cgcttcgcaa agcatgcgga cgaatcaaag acaggactcg atgaacttct cgagcttgac
     1801 gatgtccttg gcgaagttgc ggatgccttc ggcggtcttc tcggtggcca tggcgtcctc
     1861 gttgagcagc cagcggaagg atttctcatc gcagggcaca cgctcgatct ggagggacct
     1921 ggcgtgggcg gcgttgagct tcggcgtgat ggactcctcc gcgctttgca actcgccgag
     1981 cagggcgggg ctgatggtga ggaggtcgca gccgcagagt tcggtgatct cgcccttgtt
     2041 gcggaagctg gcgcccatga cctcggtgct gtggccgaaa tgcttgtagt agttgtagat
     2101 ttgcgtcacg gactgcacgc ccggatcctc cgcgcccgcg tagtcctggc cggtgctctt
     2161 tttgaaccag tcgaggatgc ggcccacgaa gggggagatg agcttgatgc cgccctcggc
     2221 gcaggccacg gcctgggcga gactgaagag cagggtgaga ttgcagttga tggactcctt
     2281 ttgcaggatc tcggcggctt tgatgccctc ccaggtggag gcgatcttga tgagcacgcg
     2341 ctcgcgcggg atgccctgct tttcataggc ggcgatgagg tggcgcgcct tgtccacgtt
     2401 gccctggatg tcgaaggaca agcgggcgtc cacctcggtg gagacacggc cgggcacgat
     2461 cttcaaaatc tcgactccga agaggatgag gatgcggtcc atgatggact cgatcttggc
     2521 ggcgccgctc agcgggctgc tcttgtgctc ggccacagcc tggtccacga gggatttgta
     2581 ttccggcttc tgggaagcct ggaggatgag ggagggattg gtggtggcgt cccggggttt
     2641 gtaggccttc atggcctcaa agtcgcccgt gtcggccacg acgatggtgt gttgtttgag
     2701 ctgttcgagc tggttcatgt gtttcaataa gggggagcag actaggggtg aatgcgggca
     2761 cgcgcaagtt ccggcctgaa agcgcattca tcacgcctgg gtgtggccgg gtccgccgag
     2821 ttttgccaca tgatcccatg ccgcaggagt tcaccagcag ccgccgtatt catgcgggga
     2881 actgatggat gatgagccgt gcagcggcaa aaaaatgtcc aaaacgcggc attcatgtcc
     2941 ttgtgcaggt catgaaccgc ctcgcccttc tgaccctcgc tctagtcatg cccgtccacg
     3001 ccgccacaga ggcggatgtc atcgtgtatg gctcgacacc cggaggcttt tgcgccgcca
     3061 ttgcggcggc gcgtgaggga gcttccgtca ttctgctgga gccgacagcc catgtcggtg
     3121 gtgtgaacac cggggggctt tgtttttcgg actcgaatca aacggtgcga agcacggtgc
     3181 tggggctgtt cgaggagtgg catcagtgca ttgaaaatga ctaccaacaa cgcggcgtgg
     3241 aactgcccta ccgggtgagt gagaaggatc acacgcattg gacttacgag ccgcatgtgg
     3301 cggcgcgtgt gacgaaggcg atgctcgacg aggcgggtgt cagggtgctt gcgaggcgtg
     3361 ttttgatgag ggctgtcaaa gaaggcgcgc gcctcacaag gctcgtcaca aatgcgggag
     3421 aattcgccgc aaaggttttc attgacgcct catatgaagg ggatctgatg gctgctgccg
     3481 gtgtgagctg gacgatcggc cgcgaggggc gcggcgaata tggcgagtcc ctggccggaa
     3541 agcagtatcc aaaggcgaga atggccatct ccggtcttga tgactccggc agaccgctgc
     3601 cgctcatcac cactggcgac gcagggcctg aggtggaggg agataaaaat gtgatggttt
     3661 acagcttccg cctctgcgtg actcgggacc ccgccaaccg cgtccctttt ccccagccca
     3721 tgaattatga tcccgcgcgc tttgagttgg tgcgccgcta cttcgcgcag gaaaaacggc
     3781 cgcatatctt gtgggacttg tatccgctgc ccggcggcaa attcgacgcg aacaacggca
     3841 tcgggaaaca gttctcgatg ggtctcgtcg gtgcctgcaa tggctggagc gaggctgatg
     3901 aggccggccg tgctgtcatc tgggaggctc acaaacaata cacgctggag ttctaccact
     3961 tcctcaccac ggatcccgct gtgccggagc atctgcgcaa agagatgagc gaactcggct
     4021 tgtgccggga tgaatttcct gaatatgagc attggtcgcc gcagctctat gtgcgcgagg
     4081 ggcggcgcat gatcggtgaa cacatcgtca cgcagaaaga catcgtggag cagccggaga
     4141 aaccggacgc cattgtcgta tcttccttcc ccattgattc gcacgactgt cagcgagtcg
     4201 gcacggggga gttcgtggtc aacgagggca ccatcatgcc cgtgcgcatg gcggggcgca
     4261 ggcacggcta cccattccac attccttacc gtgccatcac tcccaaggct tccgaatgct
     4321 cgaaccttct ggtgccagtg gcgctctcat gcacgcatgt gggcatctca tccatccgtg
     4381 tggagccgac atggatgatc ctcggccaga gtgcgggaat cgccgccgcg ctcagcgcaa
     4441 agcgtggaat cgccgtgcag gagctgtcct atccgatttt gcgcgagcgt ttgctggcgc
     4501 aaaagcaggt gcttgacctc cccgtgctgc ctgacctccc gccggggcct cctatgtctg
     4561 tgagcattga tccgaaaagt ctgcccggca tcgtggtgga cgactcggaa gccgcgctaa
     4621 agggtgcctg gagccggtct tcgaacttca agccgcacat cgggaggggc tacctgcatg
     4681 acgaccgtcg cgcggatgga ttgtctgagg ctgtttttcg attcagggtt cccaaggccg
     4741 gcaactacga tctgcgcatg gcctattccg cccacgagac acgggccacg cgcgtgcctg
     4801 tcattgtcga gaccggcggt gggaaaaccc ggctgcttgt ggatcagaca ctgccgctgc
     4861 cattggggcg gcatttccgc ggcatcggca gcgccgctct tgacgcggag acggatgtgg
     4921 tgatcacggt gacgaatcag gacacggacg gttttgtcat cgtggatgcc ctgcaggtgg
     4981 tggaggccgg gaaggattga cgcagtgcat gcctttcgac caggcgtgcc tccccattcg
     5041 ccggcgcatt gccacccgcg ctttttgcgc gcgttaccgc cccaaggatg gggagtttaa
     5101 aaatggcgtg aacccagggg aaagcccgtc ccgtttttga tttcgccatt tgacgcgccg
     5161 tgacgcgcag agtaaaacct gcccgaacat tccggcccct cgctggtcca agccatccta
     5221 tgcattccac ccgttccctg ctccgccttt ccgcagccct ggcctttctc tccatgacgg
     5281 gagcggcatt gagccaggcg ctgcccctcg accatccgtg cgcgggtctg gtgaaaaaat
     5341 accttcaaag cgtggtggct caggactgga aaacggcggc aaacatgctg ctgcccagct
     5401 ctctggagcg gaagcagaaa gagaccgtgg cgatcatcaa gaccgcgcgc accatgaccg
     5461 aggaggcgga catgctggag cgtttcggtg tgaaggacat cagggatttg gaaaagctga
     5521 ccccccagga gttttacatc accgaccgca ccgcctggca ccagcgtgtg aattcctcgc
     5581 ccgaagtcat caagcgcaag caggacacgc tcaagattga cgtgctcggc ttggtcgagg
     5641 agaaggacaa gaattacatc cacgtcaccg tgcgcacctc gcaggagacg ctcacagacc
     5701 ggattgagga gctttttctc atctcttttg tcaaggaaga cgacaagtac ctcatctggc
     5761 cggaaatgaa ggaccgtccc atcatcaccc cgctcaacga agcggcgtcc aaagaagagg
     5821 ccaaatcgaa gtgaggcctc ttaccgcgcg cggagcgatg aatcaggcat caactggcgg
     5881 cctccatgct gcgtgacacc atcgccgcca tctccaccgc tcatggcgga gcggctctca
     5941 gtgtggtgcg tgtgagcggt cccgacgccc tgggcgtttg cggcagagtc ttccgcgcgc
     6001 gccgtccgct caccgagctg gcgccgcgca ccgcattcag ggcgtgcgcc gcggactcaa
     6061 gcggcagggc ttttgacgag ggcctggtgc tcgtcttcca cgggccggcc agcttcactg
     6121 gagaagattt ggtggagttt cacggtcacg gag
//
LOCUS       DAOMYJ010000268         6143 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Prosthecobacter sp. isolate AMR_MDS_5629
            NODE_4340_length_6143_cov_21.665736, whole genome shotgun sequence.
ACCESSION   DAOMYJ010000268 DAOMYJ010000000
VERSION     DAOMYJ010000268.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867850
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prosthecobacter sp. (wastewater metagenome)
  ORGANISM  Prosthecobacter sp.
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Verrucomicrobiaceae; Prosthecobacter.
REFERENCE   1  (bases 1 to 6143)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6143)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.30
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:34:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,742
            CDSs (total)                      :: 4,684
            Genes (coding)                    :: 4,676
            CDSs (with protein)               :: 4,676
            Genes (RNA)                       :: 58
            tRNAs                             :: 55
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6143
                     /organism="Prosthecobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4340_length_6143_cov_21.665736"
                     /isolate="AMR_MDS_5629"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1965333"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..720)
                     /gene="sppA"
                     /locus_tag="PLP58_19670"
     CDS             complement(<1..720)
                     /gene="sppA"
                     /locus_tag="PLP58_19670"
                     /inference="COORDINATES: protein motif:HMM:TIGR00706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HRJ10809.1"
                     /translation="MNRTAVGCLIGALVLALLVSVGLNMAQSMLLLGQEAAGLDGREK
                     LTEKLETPGGKNATDKIARVDLEGIIASTEEGGLMGMAVAGPDFIKRALEQALEDTDV
                     KAVLLRVNSPGGEVTASDTIYAAVKKAAEKKPVVVFMDSMAASGGYYVACGATKVVAA
                     ETTLTGSIGVIMESMSYHDLFDKVGLATRTFTSGAFKDTLSGARPMREDERVYVQGLV
                     NEMYERFLGIVAQARGKPAETL"
     gene            945..1304
                     /gene="rplS"
                     /locus_tag="PLP58_19675"
     CDS             945..1304
                     /gene="rplS"
                     /locus_tag="PLP58_19675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005590628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HRJ10810.1"
                     /translation="MSNIISKIESEQLKPDVAAFNVGDTVKVHTRVVEGDKERIQIFA
                     GIVIARKGAGINEAFTVRKISYGEGVERVFPLHSPKVAKIEVTKPGKVRRSRLHYLRG
                     RIGKQAMTVKEVGQNEG"
     gene            1458..1949
                     /locus_tag="PLP58_19680"
     CDS             1458..1949
                     /locus_tag="PLP58_19680"
                     /inference="COORDINATES: protein motif:HMM:NF012257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030551 - cyclic nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HRJ10811.1"
                     /translation="MADIFSAFQDHPVRRFDAGKTVLAQGDSTGMLYILIEGRVEVVK
                     DGMAVARCADPGAVFGDLAALLAVPHTAEVRTVSAASFYEVADAAAFLERNPAVCLHL
                     CRLLARRLDAVNKYLVDVKQQFAGHDHLAMVDGMLDTLMHRQPRAHVAPKASTLRDPE
                     TAD"
     gene            complement(1917..2648)
                     /locus_tag="PLP58_19685"
     CDS             complement(1917..2648)
                     /locus_tag="PLP58_19685"
                     /inference="COORDINATES: protein motif:HMM:NF024258.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HRJ10812.1"
                     /translation="MRLLFAADLHYSLKQFDWLLAQAPDFGAVIIGGDLLDLSSALDA
                     DVQIAIVEKYLDLIRQKTMLVVCSGNHDGDCRNEADESIARWVRAARHEGLHVDGDSF
                     FIGETLVSVCPWWDGELTRSLIERQLEQDAAKDKSRWIWIHHAPPLGARTCWTGRKFI
                     GDEHLLGWIERFQPGMVFSGHIHNSPFYPEGSWIDRLGGTWVFNPGRQIGPQPATIIL
                     DLDEMTAEWSSLEGHSVRSLRIAEG"
     gene            complement(2707..5721)
                     /locus_tag="PLP58_19690"
     CDS             complement(2707..5721)
                     /locus_tag="PLP58_19690"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HRJ10813.1"
                     /translation="MNFERNSFFFLCLTAAGFCLCCCPPARAQDSLRGGLRGSVKDAD
                     FYVGVPEVVISLEPGGQSVRTDKDGRFFVNDLAPGVYRLAATAEGYIRTSQNGVVVSA
                     GSVREVDLDMTAEVVELDEFSVQDLITDESNVVPLSIGASLQSFASAISPELLKSAGS
                     SGDIGGALKRLASTAVVDSRYVVVRGLSDRYNVVVLNGARLPSSDPDKRAVNIDIFPT
                     GLIETVVSSKTAVPSMPGESTGGYLNIITKRIPKEPFLNISMSYGYNTLSTGSGQFLS
                     YKGGGTGFLGDAGSREMPSIMQDINAMPNSSAALTFNDPALSPADIEAARLSANSRAA
                     AIALRDRAMGTSYKDAPMDFSFSAIGGTRFEDFMGGTLGLIGGFTYGKKYTAEVGERG
                     FANIGAGVAQPTQQFDFQRGTESLLAGALLSASLEFNPENSVTLTWFANLSAQDDAIF
                     AFGPSLTLTELMIFREFMFYTERRLQTLQLTGEHVMPGMSDIKMDWLIAYSMSSQNQP
                     DLRKANYAFSTDPSINAYVGPGDPAPPDFERTWRRLDDTNYNVALNFEIPLDEEAKNG
                     GGGTKFKFGGNFDHSTRDYKTDNFEYVGLPDGLLVANFLGLAPGTPLLTLPPSPDDRR
                     MLTLGDLLGGIDLVDQTRDPNGLGGDLVRDNFFLARAFNIPAREVYNAYQTIPAAYGS
                     FTFNLNKKFETTVGARVEATRMTVSVERIGDGGSNFASGKSDNFELNRTDLLPSMSNR
                     WEIADKMSIRSSVSRTVARPTFKEIALVFSRDPETGNFFVGNPALQMSSIMNYDLRWE
                     YNPAPGDQFAISLFAKSIDKPIEFVNLGVFNTAENEERAVVYGFEIEMYKDLGRLMET
                     LEGFSFGLNYGFVFSKVELSQNSREIRERAGLSLDRPLQGQPDYTFNANLTHEIKDWG
                     LTSAILLNVTGPLLYTVGGQFESNLTPDIYQRPFTSLDMAFSKQITEHWSFNLRVSNL
                     LNQPRERYFEGGLPFAVTQTGTTYTMGMTGKW"
     gene            complement(5738..>6143)
                     /locus_tag="PLP58_19695"
     CDS             complement(5738..>6143)
                     /locus_tag="PLP58_19695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ10814.1"
                     /translation="RIFSVVEDRLAAPHEEQARLMWHVARLWALRGRASDMPPLLAET
                     EAAVRRMDGMHHPFAFAWLAAALELAGEKDKAAALLDEAVTSAAENPNRRTGLAGAVE
                     ISLCHARTGRVLPENVNKKLAEMAGLPAAAGE"
BASE COUNT         1154 a         1893 c         1964 g         1132 t
ORIGIN      
        1 gagcgtctcg gcgggcttgc cgcgggcctg cgcgacgatg ccgagaaagc gctcgtacat
       61 ctcgttgacg aggccctgca catacacgcg ctcgtcctcg cgcatcggcc gcgcgccgct
      121 gagcgtgtcc ttgaaggcgc cgctggtgaa ggtgcgcgtg gccaggccca ccttgtcgaa
      181 gaggtcatgg tagctcatgg actccatgat cacgccgatg ctgcccgtca gggtcgtctc
      241 cgcggccacc accttcgtgg cgccgcatgc cacataatat ccgccgctgg ctgccatcga
      301 gtccatgaaa acgaccaccg gctttttctc cgccgccttc ttcaccgccg cataaatcgt
      361 gtcggaggcc gtaacctcac cgccgggcga gttcacccgc aggagcacgg ctttcacgtc
      421 cgtatcctcc agcgcctgct ccagcgcgcg cttgatgaag tccggccccg ccacggccat
      481 gcccatcagc ccgccctcct cggtggaggc gatgatgccc tccaggtcca cccgggcgat
      541 tttgtcagtc gcatttttgc cgcccggtgt ctccagtttt tcggtcaatt tctcacgccc
      601 gtccaggccc gcagcctcct gtccgagcag gagcatggat tgcgccatgt tcaggcccac
      661 actcacgagc agggcgagca ccagcgcgcc gatcaggcag ccaacagcag ttcgattcat
      721 gctccgactc tagccgcgcc atccgctttg ccaaccgtct ttccgcagcc acgcagggtc
      781 cggaagcctg ccgggcaaat gattgcagcc acggaagccc tcaagacggg cccgcgtccg
      841 cccaaaaacc gtttgccaaa agggtgatcg catccacaat gcgcgcccct ctcaccgttc
      901 gcccctcatg ggaacaggga ggcagaggga aaaagacacc tgccatgagc aacatcatct
      961 ccaaaatcga atccgagcaa ctcaagcccg acgtggccgc cttcaatgtg ggcgacacgg
     1021 tcaaggtcca cacccgcgtc gtcgagggtg acaaggagcg catccagatt ttcgcaggca
     1081 tcgtcatcgc gcgcaaaggc gccggcatca acgaggcctt caccgtccgc aaaatctcct
     1141 atggcgaggg cgtcgagcgc gtcttccccc tgcacagccc gaaagtggcc aaaatcgagg
     1201 tcaccaagcc cggaaaagtc cgccgctccc gcctgcatta cctgcgcggc cgcatcggca
     1261 agcaggcgat gacggtcaag gaagtgggcc agaacgaggg ctgatcccct cctcggtaga
     1321 acaccgtcca cccggattat caacgccgtg tcatggcacg agccatgctg tgtcatggcg
     1381 aggcggcttg atttcctggc ggccattcct ttcagaatcc gcttttcact gggcggcaaa
     1441 ggcgtttcat gggcttcatg gctgacattt tctccgcgtt tcaagatcat cccgtgcggc
     1501 ggttcgacgc gggaaaaacc gtgctggccc agggtgacag cacgggcatg ctctacatcc
     1561 tcatcgaagg ccgcgtggag gtggttaagg acggcatggc ggtggcccgc tgcgcagatc
     1621 cgggcgcggt gttcggcgat ctggcggcgc tgctggccgt cccgcacacg gcggaggtgc
     1681 gcacggtgtc agcggcgagt ttttatgaag tggccgatgc cgccgccttt ctggagcgca
     1741 atccggcggt gtgcctgcac ctgtgccggc tgctggcgcg gcgtctggac gcggtgaaca
     1801 aatacctggt ggatgtgaag cagcagttcg ccggtcatga ccatctggcg atggtggacg
     1861 gcatgctcga cacgctcatg caccgccagc cgcgcgcgca tgtggcgccg aaggcgtcaa
     1921 ccctccgcga tcctgagact gcggactgag tgcccctcca gcgaactcca ctcagcggtc
     1981 atctcatcca ggtcgaggat gatggtagcg ggctgcggac cgatctgacg gccgggattg
     2041 aaaacccagg tgccgcccag ccggtcaatc catgaacctt cggggtaaaa cggggagttg
     2101 tgaatgtgac cgctgaaaac catgccgggc tggaaccgct caatccagcc gagcagatgt
     2161 tcgtcgccta tgaacttgcg cccggtccag caggtgcgcg ctccgagcgg aggcgcgtgg
     2221 tgaatccaga tccagcggct tttgtccttt gcggcgtcct gttcaagctg ccgctcgatc
     2281 aaggaccggg tcaattcacc gtcccaccac gggcagactg agacgagcgt ttcaccgatg
     2341 aaaaaactgt cgccgtccac atgcaggccc tcatgccgcg ccgcgcgcac ccatcgggcg
     2401 atggactcgt cggcctcgtt gcggcagtcg ccatcgtgat tgccggagca gacgacgagc
     2461 attgtcttct ggcggatgag gtcgaggtat ttctcgacaa tcgcgatctg cacatccgcg
     2521 tccagagccg aggacagatc aagcaaatca ccgccaatga tcaccgcgcc aaagtcgggc
     2581 gcctgggcaa gaagccagtc gaactgcttg agcgaataat gaagatcggc ggcgaacagc
     2641 aaacgcatgg ctgcgcgatg aaagcatctg ccgccgtgtt tgccaccacg attccccgga
     2701 gcgcgctcac cacttcccgg tcatgcccat ggtgtaggtg gtgccggtct gcgtgacggc
     2761 gaagggcagg ccgccttcga aatagcgctc gcgcggctgg ttgagcaggt tgctcacacg
     2821 cagattgaag gaccagtgct cggtgatctg cttgctgaag gccatatcca gagaggtgaa
     2881 gggacgctgg tagatgtccg gcgtcaggtt cgactcgaac tggccgccga cggtgtagag
     2941 cagcgggccg gtgacgttga gcaggatggc gctggtgaga ccccagtcct tgatctcatg
     3001 ggtgaggttg gcgttgaagg tgtagtcggg ctggccctgg agcgggcggt ccagggaaag
     3061 gccggcgcgc tcgcggatct cgcggctgtt ctggctcagc tcgaccttgg agaagacaaa
     3121 gccgtagttc agcccgaagg agaagccctc cagagtttcc atgagccggc cgaggtcctt
     3181 gtacatctcg atctcgaaac catagaccac ggcgcgctcc tcgttttccg ccgtgttgaa
     3241 gacgccgagg ttcacgaact cgatgggctt gtctatgctt ttggcgaaca atgagatggc
     3301 gaactggtcc cccggtgcgg ggttgtattc ccagcgcagg tcgtagttca tgatggagga
     3361 catctgcagc gcgggattgc cgacgaagaa gttcccggtt tccgggtcgc gtgagaaaac
     3421 gagggcgatc tccttgaagg tgggccgggc caccgtgcgg ctgacggacg agcggatgga
     3481 cattttgtcg gcgatctccc agcggttcga catgctgggc agaaggtcgg tgcggttgag
     3541 ttcgaagttg tccgacttgc cgctggcgaa attgctgccc ccgtcaccaa tgcgctccac
     3601 actgaccgtc atgcgcgtgg cttccacgcg cgcgccgacg gtggtctcga actttttgtt
     3661 caggttgaac gtgaaagagc cgtatgccgc cggtatggtt tggtatgcgt tgtaaacctc
     3721 gcgggccggg atgttgaagg cgcgggcgag gaagaagttg tcgcgcacaa ggtccccacc
     3781 aagaccattc ggatcacgag tctgatcaac caggtcaatg ccgccaagca agtcaccgag
     3841 ggtaagcatg cggcggtcgt cagggctggg tggaagggtg aggagaggag tgcctggagc
     3901 caggccgagg aaattggcca ccagcagacc atccggcaga cccacatact cgaagttgtc
     3961 ggttttataa tcacgcgtgc tgtggtcgaa gttgccgccg aatttgaact tggtgccgcc
     4021 gcccccgttc ttcgcctcct cgtcgagcgg gatttcgaag tttaacgcca cattgtagtt
     4081 cgtgtcgtcg agccggcgcc aggtgcgctc gaagtcgggc ggcgctgggt cgccggggcc
     4141 gacgtaagcg ttgatgctgg ggtcggtgct gaaggcatag ttggccttgc gcaagtcggg
     4201 ctggttctgg gacgacatgc tgtaggcgat gagccagtcc attttgatat cgctcatgcc
     4261 gggcatgacg tgctcgccag tgagctgaag tgtctgaaga cggcgctcgg tgtaaaacat
     4321 gaactcgcgg aagatcatca actcggtgag ggtgagcgag gggccgaagg cgaagatggc
     4381 atcatcctgg gcggagaggt tggcgaacca ggtgagggtg acactatttt cgggattgaa
     4441 ctccagcgag gcgctgagca aagcacctgc gagcagactc tccgttccgc gctggaagtc
     4501 gaactgctgc gtaggctggg cgacaccggc cccgatgttg gcaaagccgc gctcacccac
     4561 ttcggcggtg tattttttgc cataggtgaa gccgccgatc agaccgagcg tgccgcccat
     4621 gaagtcctca aaacgcgtgc cgccaatggc gctaaagctg aagtccatgg gcgcgtcctt
     4681 gtagctggtg cccatggcgc ggtcccgcaa ggcgatggcc gcggcgcggg agttggcgga
     4741 aaggcgcgcc gcctcgatgt cggcgggcga gagggcggga tcgttgaaag tcagcgcggc
     4801 ggagctgttg ggcatggcgt tgatgtcctg catgatggag ggcatttcgc gggaacccgc
     4861 gtctccaagg aagcccgtgc cgccgccttt gtagctcaag aactggccgc tgccggtgga
     4921 cagcgtgttg tagccgtaag acatggagat gttaagaaag ggctctttcg gaatgcgctt
     4981 ggtgatgatg ttcaggtagc cgccagtgga ttcgccaggc atggagggca ctgcggtttt
     5041 ggaactgacg accgtctcga tgagacctgt ggggaagatg tcaatgttca ccgcgcgctt
     5101 gtcggggtcc gagctgggca ggcgcgcgcc attgagcacg acgacgttgt aacggtcgct
     5161 caatccgcgc acgacgacgt agcggctgtc caccacggcg gtgctggcca ggcgcttgag
     5221 cgcgccgccg atgtcgccgg aggagccggc gcttttcagc agctccggcg agatggcgct
     5281 ggcgaaggac tgcaaggacg cgccgatgct caggggcacg acattgctct catccgtgat
     5341 cagatcctgc acactaaact cgtccagctc caccacctca gcagtcatgt cgagatccac
     5401 ctcccgcaca ctgccggcgc tgacgaccac gccgttttgg ctggtgcgga tatagccctc
     5461 ggccgtggcg gcgagccgat agacgccggg tgcgaggtca ttgacaaaaa accgcccgtc
     5521 cttgtccgtg cgcacgctct gccccccagg ctcaagactg atgacgacct ccggcacgcc
     5581 gacataaaag tccgcgtcct tcaccgagcc gcgcaaacca ccgcgcaggg agtcctgggc
     5641 gcgtgcggga ggacagcagc agagacaaaa gcctgcggcg gtgagacaga ggaaaaagaa
     5701 ggagttccgc tcgaagttca tgctcgcatc cgcccgctca ttcacctgcc gccgccggca
     5761 gtcccgccat ttccgccaat tttttgttca cattctccgg caaaacgcgc ccggtgcgcg
     5821 catgacagag gctgatctcc accgcgccgg cgaggcccgt gcgccggttc ggattttccg
     5881 ccgcgcttgt caccgcctca tcgagcaggg ccgccgcttt gtctttctca cccgccagtt
     5941 ccagcgccgc cgccagccag gcgaaggcaa acgggtgatg catcccgtcc atgcggcgca
     6001 ccgccgcctc cgtctcagcc agcaggggcg gcatgtccga ggcgcggccc ctcagcgccc
     6061 aaaggcgagc cacatgccac atcagccggg cctgctcctc atgcggagcc gcgagccggt
     6121 cttcgacgac actaaatata cgg
//
LOCUS       DAOMYJ010000269         6075 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Prosthecobacter sp. isolate AMR_MDS_5629
            NODE_4418_length_6075_cov_20.744352, whole genome shotgun sequence.
ACCESSION   DAOMYJ010000269 DAOMYJ010000000
VERSION     DAOMYJ010000269.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867850
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prosthecobacter sp. (wastewater metagenome)
  ORGANISM  Prosthecobacter sp.
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Verrucomicrobiaceae; Prosthecobacter.
REFERENCE   1  (bases 1 to 6075)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6075)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.30
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:34:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,742
            CDSs (total)                      :: 4,684
            Genes (coding)                    :: 4,676
            CDSs (with protein)               :: 4,676
            Genes (RNA)                       :: 58
            tRNAs                             :: 55
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6075
                     /organism="Prosthecobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4418_length_6075_cov_20.744352"
                     /isolate="AMR_MDS_5629"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1965333"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..915)
                     /locus_tag="PLP58_19700"
     CDS             complement(<1..915)
                     /locus_tag="PLP58_19700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009958503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ10815.1"
                     /translation="MRETKLLLFALLPLFCGQTQALDDARNIDKAWEVVWSRFYLPKV
                     QTFGDYLSSYEKGKEQAHLPTAEEVRRQYPNPCGYSTGMEDGAILGGAMLSVLCDRFE
                     VTKDESLREKAANVFAGLRRCVAVHGVKGFVARNVCPEDGRSTYINSSRDQVTHFVHG
                     LWQYFHSALCDEVTKVQIRVILSDVAERMIKFVTPENDYDFCRADGTRCPLGICRMWK
                     VQAHEAARLPMVYAAAWDVTKNERYRAEWRKYAAEAIAQSASPVENKPAYALLQMQCS
                     LELLHAVEPDKLQMQCSLELLHAVEPDLD"
     gene            complement(1290..1652)
                     /locus_tag="PLP58_19705"
     CDS             complement(1290..1652)
                     /locus_tag="PLP58_19705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ10816.1"
                     /translation="MKPKIIKTKEEYTEALARVESLMDAKPGTAKEEELELWSLLVER
                     YEQEHFPIDMPDPVEAIKFRMEQKGLQQKDLVQYFPGKNRVSEVLNHKRPLSIGMIRA
                     LHRGLRIPAEVLLREATV"
     gene            complement(1656..1949)
                     /locus_tag="PLP58_19710"
     CDS             complement(1656..1949)
                     /locus_tag="PLP58_19710"
                     /inference="COORDINATES: protein motif:HMM:NF021431.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HigB family
                     toxin"
                     /protein_id="HRJ10817.1"
                     /translation="MRVIARKTLRDFYEQHADSKSALEAWFHEAVAARWQGPQEIKSR
                     YPSADILPGNRVVFNIKGNSYRLIVKIHYNTSIVFIRFVGTHAEYDKIDATAI"
     gene            complement(2019..4640)
                     /locus_tag="PLP58_19715"
     CDS             complement(2019..4640)
                     /locus_tag="PLP58_19715"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF012403.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HRJ10818.1"
                     /translation="MPKTSASRKAKRAKKKSTAIPLPSAGDWRSTDEIEVLRRIQRAR
                     EEKHSISNLNAEHPVFSTFAVKSPSGMTYQVEIRDLASRSFSCTCPDFRTAGLGTCKH
                     VEATLIWLKRRHKADYKLAETRSSPHLHLVPDGERLRIERDLKRVPASLRALFDEAGF
                     LIGDPHEVLPKLRRTAKVRISQDVEPFLESRGHAEERRELRRDYETGVVEGRHPEHVT
                     LHPLYPYQREGMLHLAFGERALLADEMGLGKTIQAVAACALLHHLGKAKRVLVVTPAS
                     LKTEWEEQIKRFTTLGLQLVFGPRTARLKAYTASEPPFFTIVNYEQVVTDTLDINQHL
                     KPDIVVLDEAQRIKNWATKTAQAVKRLQSRYAFVLTGTPIENRIDELYSIVDFLDPSL
                     LGPLFRFNREFYQLDENGRPKDYQNLAALRERVRPVLLRRRKHDVETELPIRTDRQHF
                     IQLTPAMRGEYDEVKQQVSQLVQKSLRRPLTKKESDLLLLFLGMMRMICDSPSIIKGH
                     DCQDCPKMDELAGILDEALADPYVKVIIFSEWEGMLKKVRTWAEKNGIGFAWHTGSVP
                     QQKRRAEIMAFRNDPACRLFLSTDSGGVGLNLQNASIVINCDLPWNPARLEQRIARAW
                     RKHQKRPVTVINLVAENTIESAMLETLATKANLAEGVLDGSEESLSKARLKRGNDANL
                     ARLKQFISETPASPATPQVKPPPSDPALVFAERVTSVLGQSLLHCQEAMLPGESSPVL
                     LTVLRDTSRSAAVSALFHETQWSGNTPKLHVLDEHTWQSLQQLAQIGLITFNTRATRH
                     LSGEQPPAPPKPTLTPEQLQRITDLRTFAAKKEKVAQFLIAEEMHDDAIPHQQAALSA
                     LRQADFIESGDEDDIPF"
     gene            complement(4680..>6075)
                     /locus_tag="PLP58_19720"
     CDS             complement(4680..>6075)
                     /locus_tag="PLP58_19720"
                     /inference="COORDINATES: protein motif:HMM:NF024642.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HRJ10819.1"
                     /translation="PLLLDYKQERCARLDLEPDPDAENLARDLRLRVITGVAGCGKTL
                     VLVHRAALLATHFPQARVLLVSFNRPLINDLRRRLARHHAGSRVECCTFNQWLGRVAR
                     SAGDMMSEPENLRWIERERSPMTSLSKLSPEWLRDELRWMCDHALADETYLTAERKGR
                     GTRLSARQRRELLELLYRYRDHLRQNGRADWSEWPLTVRENPPASLFAEKFDHLLIDE
                     AQFFAPVWLELLKSALKPGGHLFLCADPTQGFLKRRLSWSAVGLDVRSRSHKLEKPYR
                     STRAILEFARDFYRSRLSEEDEPLNLPAPEWLETLEPGIPPILQPGGAGQDQLRRLTA
                     DLKALRQSGMPASHILILVAGRTMTVQTVMQHLNTSLGSNSASSVRDDHTPEESIGVA
                     HLMAATGLERPVVFLLGCDDLIATESNPLLTDEERAELIQDHTRQIYVGITRGMERVV
                     IYEDRLLASSNDRA"
BASE COUNT         1117 a         1657 c         2014 g         1287 t
ORIGIN      
        1 gtccaaatca ggctcaacgg catggagaag ctccagcgag cattgcatct ggagcttatc
       61 aggctcaacg gcatggagaa gctccagcga gcattgcatc tggagcagtg cgtaggcggg
      121 cttgttctca acggggcttg ccgactgcgc gatggcctcc gccgcatact tccgccattc
      181 ggcgcgatag cgttcattct ttgtcacatc ccacgccgcc gcgtaaacca tcggcagccg
      241 cgccgcctca tgcgcctgca ccttccacat gcggcagatg ccgagtgggc agcgcgtgcc
      301 atcggcgcgg cagaagtcgt aatcgttctc cggcgtcaca aacttgatca tgcgctcagc
      361 cacatcggac aggatcacgc ggatctgaac cttcgtgacc tcatcgcaca acgcgctgtg
      421 aaaatactgc cacaggccgt gcacgaaatg cgtcacctga tcgcgagacg agttgatgta
      481 ggtgctcctg ccatcctctg gacacacatt gcgcgccaca aagcccttca cgccatgcac
      541 cgcgacgcat cgccgaagac cggcgaagac gttcgcggcc ttttcacgca acgattcatc
      601 cttcgtgacc tcaaatcgat cacacagcac actgagcatc gccccgccga ggatcgcacc
      661 gtcctccatg cctgtgctgt agccgcaggg attcggatac tgccgcctca cctcctccgc
      721 cgtcggcaga tgcgcctgct ccttgccttt ctcgtagcta ctgaggtaat cgccaaaagt
      781 ctgcaccttg ggcaggtaaa accggctcca gaccacttcc caggctttgt cgatgtttcg
      841 cgcatcgtcc agcgcctgag tttggccgca aaaaagcgga aggagcgcaa aaagaagaag
      901 tttggtctct ctcatgaaaa gtgtgaggtg tcagcttcaa aatgggatgt cgtcattgtc
      961 gtggatggtt tcgattcgat gggctttatc aagggcattt ccggccgcct cttgatgcga
     1021 agcggcttcg tcgtccagac cttcggcgat gagcagttgg gcgacttttt tttggcagcg
     1081 agagcacgga ggtcggcgat gcgctggagt tgctcggggg tgagggcggg tttggctgga
     1141 ggtggtgggc gcgggtgttg aaggtgatga ggccgatctg agcgagctgc tggaggctca
     1201 gtcaggtggg ttcgtcgtga atgctcagct tcagggacca aagcagaaat gaggactgga
     1261 actcgggcca aatgttgcac gatggcgcat catactgtcg cctctctcag cagcacttcc
     1321 gccgggattc gaagcccgcg gtgcagcgcc cggatcatgc cgatgctgag cgggcgcttg
     1381 tggttgagca cttcggacac ccggttcttg cctgggaaat actgcaccag atctttttgc
     1441 tgaaggcctt tctgctccat gcggaatttg atcgcttcta ccggatcggg catgtcgatg
     1501 gggaaatgct cctgctcata acgctccacc agcagggacc acagttcgag ttcctcctcc
     1561 ttggccgtgc cgggcttggc atccatcagg ctctccacac gggccagggc ctccgtgtat
     1621 tcctccttag ttttgatgat tttcggtttc atgggctaga tggctgtggc gtcgatcttg
     1681 tcgtattcgg cgtgcgtgcc gacaaagcgg atgaagacaa tgctggtgtt gtagtggatt
     1741 ttcacgatca gacggtagct gttccctttg atgttaaaca ccacccggtt gccgggcagg
     1801 atgtcggcag acgggtagcg gctcttgatt tcctgcggcc cctgccagcg ggcggccacg
     1861 gcttcgtgaa accaagcctc cagggcggac ttgctgtcgg cgtgctgttc ataaaagtcg
     1921 cggagcgttt tgcgggcgat gactctcatg ggcagaatcg aggtttaagg cttaaaatca
     1981 agaacgaagt tcccatcatg ggaacttcgt caatgatttc aaaaggggat gtcgtcttca
     2041 tctcccgact cgatgaaatc ggcctgacgt agagcgctca gcgctgcttg ctgatgtggg
     2101 atggcgtcgt cgtgcatttc ttcagcgatg agaaactgag cgactttttc cttcttggcc
     2161 gcaaaggtgc ggaggtcggt gatgcgctgg agttgctcgg gggtgagggt gggcttgggc
     2221 ggcgcgggtg gttgctcgcc gctgaggtgg cgggtggcgc gggtgttgaa ggtgatgagg
     2281 ccgatctggg cgagctgctg caggctctgc caggtgtgct cgtcgaggac gtggagcttg
     2341 ggggtgttgc cgctccactg ggtctcgtgg aagagggcgc tgacggcggc gctgcgggag
     2401 gtgtctcgca gcacggtgag gaggacgggg ctggattcgc cgggcagcat ggcttcctgg
     2461 caatggagaa ggctttgacc tagcacgcta gtgacacgct ccgcaaaaac gagcgcggga
     2521 tcactcggcg gcggcttcac ttgcggtgtg gcgggtgagg cgggcgtttc gctgatgaat
     2581 tgtttcaacc gggcgaggtt ggcgtcgttg ccacgcttga gcctggcttt ggagagggat
     2641 tcctcgctgc cgtcgagcac gccttcggcg aggtttgcct tggtggcgag ggtttcgagc
     2701 atggcggact cgatggtgtt ttcagcgacg agattgatga cggtgacggg acgcttctga
     2761 tgcttgcgcc aggcgcgggc gatgcgctgc tccagccggg cgggattcca cggcaggtcg
     2821 cagttgatga cgatgctggc gttttgcaaa ttgaggccca cgccgccgga gtcggtgctg
     2881 aggaagaggc ggcaggccgg atcattgcgg aaggccatga tctcggcgcg gcgtttttgc
     2941 tgcggcacag agccagtgtg ccaggcgaag ccgatgccgt ttttctccgc ccaggtgcgc
     3001 acttttttca acatgccttc ccattcggag aagatgatga ctttcacgta ggggtcggca
     3061 agggcttcat cgaggatgcc ggcgagttcg tccatcttcg gacagtcctg gcagtcgtgg
     3121 cctttgatga tgctgggcga gtcgcaaatc atgcgcatca tgccgagaaa cagcagaagc
     3181 agatcagact cctttttcgt caaaggacgg cgcagggact tctgcacgag ctgggagacc
     3241 tgctgcttga cctcatcata ctccccacgc atggcggggg tgagctggat gaagtgctgg
     3301 cggtcggtgc ggatgggcag ctcggtttcg acatcgtgct tgcggcggcg gagcagcacg
     3361 gggcgcacgc gctcgcggag ggcagccagg ttttggtaat ccttcgggcg gccattctcg
     3421 tcgagctggt agaactcgcg attgaagcgg aagagcggcc cgagcaggct gggatcgagg
     3481 aaatcgacga tggagtagag ttcgtcgatg cggttctcga tcggggtgcc ggtaagcacg
     3541 aaggcgtagc ggctttgcag gcgcttgacg gcctgggcgg ttttggtggc ccagtttttg
     3601 atgcgctggg cttcgtcgag caccacgatg tcgggcttca aatgctggtt gatgtcgagc
     3661 gtgtcggtga cgacctgctc atagttcacg atggtgaaaa acggcggctc agaagcggtg
     3721 taggccttga gccgggcggt gcgcggacca aaaacgagct ggaggccgag ggtggtgaag
     3781 cgcttgatct gctcttccca ttccgttttc agcgaggcgg gtgtgacgac gagcacgcgc
     3841 ttcgctttgc ccaaatgatg cagcagcgca cacgcggcca cggcttggat cgttttgccg
     3901 aggcccatct catccgccag cagcgcccgc tcgccaaagg ccaggtgcag catgccctcc
     3961 cgctgatacg ggtagagcgg atgcagcgtg acgtgctcgg gatggcggcc ctcgacgacg
     4021 ccggtctcgt agtcacggcg gagctcgcga cgctcctcag catggccgcg ggattcgaga
     4081 aaaggctcca cgtcctgcga gatgcggact tttgccgtgc ggcggagctt tggcaggact
     4141 tcatgcggat caccgatgag gaagccagcc tcatcaaaca gcgcacgcaa cgaagcaggc
     4201 acacgcttga ggtcgcgctc gatgcgcagc ctctcgccat ccggcacgag atggagatgc
     4261 ggcgaggaac gtgtttccgc gagcttgtag tctgccttgt ggcggcgttt cagccagatg
     4321 agcgtggcct cgacatgctt gcaggtgccc aggcctgcgg tgcggaagtc cgggcaggtg
     4381 caggaaaacg agcgactggc tagatcgcga atctcaacct ggtaagtcat gccgctgggg
     4441 gacttcaccg cgaaggtgga gaagaccgga tgctccgcgt tgagattcga gatggagtgt
     4501 ttttcttccc tcgcccgctg aatgcggcgg agcacttcga tctcatccgt gctgcgccag
     4561 tctcctgcgg atggaagcgg gatggctgtg gacttcttct tggcgcgttt ggcttttctg
     4621 gaagcggatg tcttgggcat gagggcgtga gctttggata gaaatgaagc cagggcaaat
     4681 catgctctgt catttgacga tgccaggagc ctgtcctcat agatcaccac acgctccatg
     4741 ccacgggtga tgccgacgta gatctggcgg gtgtggtcct ggatgagttc ggcgcgttct
     4801 tcgtcagtga ggagcgggtt ggactcggtg gcgatgaggt catcacagcc gaggaggaag
     4861 accaccggcc tctccagccc ggtcgctgcc ataaggtgag ccacgccgat gctttcctcc
     4921 ggcgtatgat catcacgcac ggagctggcg gagtttgatc cgaggcttgt attgaggtgc
     4981 tgcatgacgg tctgaacagt catggtgcgg ccagccacga ggatgaggat gtggctcgct
     5041 ggcatgccgc tttgtcgcaa agctttcaaa tcggcggtca agcgacgcag ttgatcctgt
     5101 cccgctcctc caggctgcaa aatgggtgga atgcccggct ccagtgtctc cagccattca
     5161 ggcgcgggaa ggttcagcgg ctcgtcctct tcagaaagcc ggctgcggta aaaatcacgc
     5221 gcaaactcca ggatggcgcg tgtgcttcgg taaggttttt cgagcttgtg agagcggctg
     5281 cgcacatcca gcccgacggc gctccaggac aggcgtcgct tcaaaaaacc ttgggtggga
     5341 tcagcacaca aaaagaggtg cccgccgggt ttgagagccg atttgagcaa ttcgagccac
     5401 accggcgcga agaactgcgc ctcgtcgatg agcagatgat caaacttctc cgcgaaaagg
     5461 gatgcaggtg gattttcacg cacggtgaga ggccactcgg accagtcagc acgcccgttt
     5521 tgccgcagat ggtcgcggta acggtagagc aactccagca gttctcgccg ttgtctggcg
     5581 ctgaggcggg tgccgcggcc tttgcgctcg gcggtgaggt aggtttcatc cgcgagtgca
     5641 tggtcacaca tccagcgcag ctcatcacgc agccactcgg ggctgagttt tgacagtgag
     5701 gtcatgggcg agcgttcacg ctcgatccag cgcaggttct ccggctcgct catcatgtca
     5761 ccagcggagc gggccacacg tccgagccat tgattgaaag tgcagcactc cacgcggctg
     5821 cctgcgtgat gccgcgccag acgacggcgg aggtcgttga tgagcggccg gttgaagctg
     5881 acgagcagca cgcgtgcttg tgggaagtgt gtggcgagca aggcggcgcg atgcacgagc
     5941 acgagcgtct ttccacagcc cgccacgccg gtgatgacac gcaggcgcag atcacgcgcg
     6001 aggttttctg catcaggatc gggttccaga tcgagacgtg cacagcgctc ttgtttgtag
     6061 tcgagcagca gtggc
//
LOCUS       DAOMYJ010000270         6057 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Prosthecobacter sp. isolate AMR_MDS_5629
            NODE_4431_length_6057_cov_23.633122, whole genome shotgun sequence.
ACCESSION   DAOMYJ010000270 DAOMYJ010000000
VERSION     DAOMYJ010000270.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867850
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prosthecobacter sp. (wastewater metagenome)
  ORGANISM  Prosthecobacter sp.
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Verrucomicrobiaceae; Prosthecobacter.
REFERENCE   1  (bases 1 to 6057)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6057)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.30
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:34:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,742
            CDSs (total)                      :: 4,684
            Genes (coding)                    :: 4,676
            CDSs (with protein)               :: 4,676
            Genes (RNA)                       :: 58
            tRNAs                             :: 55
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6057
                     /organism="Prosthecobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4431_length_6057_cov_23.633122"
                     /isolate="AMR_MDS_5629"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1965333"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..587
                     /locus_tag="PLP58_19725"
     CDS             <1..587
                     /locus_tag="PLP58_19725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009958012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] synthase III
                     C-terminal domain-containing protein"
                     /protein_id="HRJ10820.1"
                     /translation="NDAPDSILANSLFADGAEAAIVSARAPEPRGAAYRLRGFESALM
                     ASSEQEMAWDIGDTGFNIVLSSYVPDIIGANIRELLGGVLLRQNLGFGQIGEWAVHPG
                     GRAILDKVQQSLALEPDALDASRAVLRDYGNMSSATILFVLKELLDKAESEPAMTCAL
                     AFGPGLTVEAAVLERLGCTVPPAQSRKETRTESE"
     gene            584..1777
                     /locus_tag="PLP58_19730"
     CDS             584..1777
                     /locus_tag="PLP58_19730"
                     /inference="COORDINATES: protein motif:HMM:NF013646.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HRJ10821.1"
                     /translation="MNHDIAIVGAGPAGLLLAGLLGQGGLRVLIVDKRAGPLAHSQAI
                     GITPPSLEILARLGLDAEFVRRGVPIRDCHVHGRSGYLGCASFREIPGAHRHILSLPQ
                     QVTLSLLESKIATMPNVELHRGIEVTRIGQDAERVALSGGGFSSTARWLVGCDGHRSR
                     VRELIQMRCKGASYGCHFLMGDFTDRTTLGDEAHLWFTHEGAVESFPLPGGRRRWIVQ
                     TAQAMPETERGFISGIVRERTGLDLPAGDQHNQSPFSPRWMQAGQYHDGRVILCGDAA
                     HLMSPIGGQGMNTGWADAEFLAGALLAIEQRGQPALPLLEAYDHCRHRASNAAIRRAA
                     LGMWLGTRTGRAQSWLRDVFLRELVLKGPLSRRVGPHFAMLTIPCGTLAQVPEKDRAR
                     LTPAA"
     gene            complement(1761..3377)
                     /locus_tag="PLP58_19735"
     CDS             complement(1761..3377)
                     /locus_tag="PLP58_19735"
                     /inference="COORDINATES: protein motif:HMM:NF036494.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HRJ10822.1"
                     /translation="MKPIFLTALPALAVCLCAPAQDAPQTPAPKRPAGKSAPPSPGQL
                     PFGPAKPLGAIVPPAGLPGMAPVIQTSALLKVNVTYQSHSQRIPWQKESPGSRRGLGV
                     VLAGNRVLVTGQMVGDATYIELELPESGQKLPAKVEAVDYEANVALLAPASPEKEAAF
                     FAGLKPMSVDTSARIGDALNVWQTGRVGELIVTPMRVGKVMTQSYAVPNASFLVYEAQ
                     GIIRSEANSFTLPVVKGGRLAGLLLRYDSKNQVATILPAPIIEHFLKDVAKAPYEGFP
                     SLGVEFQTTLDEQFRDYLGLAPDQPGVYISKVVKGASADAAGVKKGDIMLSINGARID
                     SRGDYADPQYGALSISHVVRGRAYVGDEVEIKVLREGRELTLKSKLTRKLPEDYLVLP
                     YLYDQGAKYVLHGGLLFQELTQPYLGSFGREQLGGPLLRLSRVASDPELFEEDGRKRV
                     VFLSAVLPTPTTQGYERLSGQVVTTVNGVKITEIKDLAEAFTKPKDGIHTVELREFPF
                     IVHLDAASAERDNLQLMNGMFRIGSLKRLE"
     gene            complement(3415..4887)
                     /locus_tag="PLP58_19740"
     CDS             complement(3415..4887)
                     /locus_tag="PLP58_19740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009958240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HRJ10823.1"
                     /translation="MKFSLLPLAALAAVFLPAALRGQAIDLSTDEWEGVVNIDCSVLI
                     PDYREPWNAGQPSGGSGTGFLIGKNRFLTNAHVVSNATKLVLRTSNDPRPHPARIVHI
                     AHDCDLAILEAEDGEPFEKLKPLSIGGLPKLNTEVIAIGYPIGGDRISVTRGVVSRID
                     FRPYSHTQVDSHLAIQVDAAINPGNSGGPVMQDGKVVGVAFQGFSGRVAQNVGYIIPV
                     PVIERFLKDVEDGKYDHYVDFAVSDFAIENIAQRKALGLVDNTGVMIADVEPAGSAGG
                     ILQRGDVLLSINGNPVFNNGLIRMEGEMVNMNEVVERKFAGDVLDLVILRGGKRQETK
                     VALKRFDPYVRLGEQYNQRPRYIVYAGLVFQPMDKSLMDSHQINDSTASYIFDNFLTE
                     KLYVERPEPVILTSILADEVNTHLSPYAHSVVDEINGMKIRTLKDVREALSKEEEKFV
                     VIKLLEKGRPLVLRRDLAETAHPRIMQTYSIRDDAYLGLE"
     gene            complement(5187..5891)
                     /locus_tag="PLP58_19745"
     CDS             complement(5187..5891)
                     /locus_tag="PLP58_19745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ10824.1"
                     /translation="MKISSPLQAAIMTALALTIGNQALGQRPVFVPDDRYPVAQRQSY
                     LLQEVECLQSTTRTLHQAFVSYCKRYKVAAASPDGQLMRALVDLQLDVSRLASQIRAH
                     TGNASIPLGEAYRSFHMAEYASRDSQLMAVQAGFIRSMFPYFRDIDQHLSEIGRLGYR
                     NPMLRPLEMDGRSYGSRGSGVQERHFELPPLVLPQPIPSGPVPPRPDRGRDPYEREDK
                     LDLGDVLKQLFRGRLN"
BASE COUNT         1049 a         1878 c         2043 g         1087 t
ORIGIN      
        1 tgaatgacgc gccggactcc atcctcgcga actcgctctt tgccgacggc gccgaggcgg
       61 ccatcgtcag cgcgcgcgcg ccggagccgc gcggcgccgc ataccgcctg cggggtttcg
      121 agtccgcgct catggcctcc agcgagcagg agatggcctg ggacatcggc gacacgggtt
      181 tcaacatcgt cctctccagc tatgtgccgg acatcatcgg ggccaacatt cgcgagctgc
      241 tcggcggcgt gctgctgcgg cagaacctcg gctttggaca aatcggggaa tgggccgtgc
      301 atcccggcgg acgggcaatc ctcgacaaag tccagcaaag tcttgctttg gaaccagacg
      361 cgctggacgc ctcccgcgcc gtgctgcggg attacgggaa catgagcagc gccaccatat
      421 tgtttgtcct caaggagctg ctggacaagg ccgagagcga gcctgcgatg acctgcgcgc
      481 tggctttcgg ccccggtctc acggtggagg ccgccgtgct ggagcgcctg ggctgcacgg
      541 tgccgcccgc tcaatcacgc aaggaaaccc ggactgagtc ggaatgaatc atgacatcgc
      601 catcgtcggc gcggggccgg ccggtttgct gcttgcgggg ctgcttgggc agggcgggct
      661 gcgggtgctc atcgtggaca agcgcgccgg gccgcttgcg cattcgcagg ccatcggcat
      721 cacgccgcca tcgctggaga tcctggcgcg cctcggtctc gatgctgagt ttgtccgtcg
      781 tggcgtgccg atccgtgact gtcatgtgca tgggcggtcg ggttatctcg gctgcgcgtc
      841 gttccgtgag attccgggcg cgcatcgcca cattctctcg ctgccgcagc aggtgacgct
      901 ctctctgctg gagtcaaaaa tcgccacgat gccgaatgtg gagctgcatc gcggcattga
      961 ggtcacccgg atcgggcagg atgccgaaag ggtggcgctg agtggcgggg ggttcagctc
     1021 gaccgcccgc tggctcgtcg gttgcgacgg gcacagaagc cgcgtgcgcg agctgatcca
     1081 aatgcgctgc aagggcgcgt cctatggctg ccattttctg atgggtgact tcaccgaccg
     1141 caccacgctc ggtgacgagg ctcatctgtg gttcacgcac gagggagcgg tggagtcttt
     1201 tcccctgcca ggcggccgcc gccgctggat tgtgcagaca gcgcaggcca tgccggagac
     1261 agaacgcggc ttcatcagcg gcatcgtgcg cgaacgaacc gggcttgatc tgcccgccgg
     1321 ggatcagcac aaccaaagcc ccttctcacc gcgctggatg caggccgggc agtatcacga
     1381 cgggcgtgtc attctctgcg gcgatgcggc gcatctgatg agtcccatcg gcggccaggg
     1441 catgaacacc ggctgggcgg acgccgaatt cctcgccggg gcgctgctcg ccatcgagca
     1501 gcgcggccag cccgcgctgc cattgctcga agcctatgac cactgccgcc accgcgcctc
     1561 caacgcggcc atcaggcggg cggcgctggg catgtggctc ggcacgcgca cggggcgcgc
     1621 gcagtcctgg ctgcgtgatg tgtttctgcg cgaacttgtg ctgaaagggc cgctctcgcg
     1681 tcgagtcggg ccgcatttcg cgatgctgac gattccctgc gggacgctgg cgcaagtgcc
     1741 tgaaaaagac cgcgcccgcc tcactccagc cgcttgaggc tgccgatgcg gaacatgccg
     1801 ttcatgagct ggaggttgtc ccgctcggcg gaggccgcgt cgagatgcac gatgaaggga
     1861 aactcccgca actccacggt gtggatgccg tctttgggct tggtgaaggc ttcggccagg
     1921 tccttgatct cggtgatctt cacgccgttg acggtggtga ccacctggcc gctgaggcgc
     1981 tcgtagccct gggtggtcgg cgtgggcagc acggcgctga ggaagaccac gcgcttgcgg
     2041 ccatcctcct cgaagagttc ggggtcggag gccacgcgcg agaggcgcag cagcgggccg
     2101 ccgagctgct cgcgcccgaa ggagccgagg tagggctggg tgagctcctg aaagagcagc
     2161 ccgccatgca gcacatactt cgccccctga tcgtaaagat aaggcagcac caggtaatcc
     2221 tccggcagct tgcgggtgag cttgcttttg agcgtcaact ccctgccctc gcgcaggact
     2281 ttgatctcca cctcgtcgcc gacataggcg cgtccgcgca cgacatggct gatgctcagc
     2341 gctccgtatt gcggatcggc gtagtctccg cgcgagtcaa tgcgggcgcc gttgatggaa
     2401 agcatgatgt cgcccttctt gacacccgcc gcgtcggcgg aggcgccttt gaccaccttg
     2461 ctaatataga cacccggctg gtcgggcgcg aggccgaggt agtcgcggaa ctgctcgtcg
     2521 agagtggtct ggaattcgac gcccagcgag gggaagccct cataaggcgc cttcgccacg
     2581 tccttgagga agtgctcgat gatgggcgcg ggcaggatgg tggccacctg gttcttcgag
     2641 tcgtagcgca gcagcaggcc ggcgaggcgg ccgcctttga ccacgggcag ggtgaagctg
     2701 ttcgcctcgc tgcggatgat gccctgcgcc tcatagacga ggaagctggc attgggcacc
     2761 gcataactct gggtcatcac cttgcccacg cgcatggggg tgacgatgag ttcgcccacg
     2821 cggccggtct gccagacatt gagcgcgtcg ccaatgcggg cggaggtgtc caccgacatg
     2881 ggcttcagcc cggcgaaaaa ggcggcctcc ttctccggcg aggccggggc gagcagggcg
     2941 acattcgcct cgtaatccac cgcctcgact ttggcgggca gcttctgccc gctctcgggc
     3001 agctccagct cgatgtaggt ggcgtcaccg accatctggc cggtgaccag cacgcggttg
     3061 cctgcgagca ccacgcccag gccgcgacgg ctgccggggg actccttctg ccaggggatg
     3121 cgctggctgt gggactggta ggtgacgttc accttcagca gggcgctggt ctggatgacg
     3181 ggagccatgc cggggagccc cgccggcggc acgatggctc ccaacggttt ggcggggcca
     3241 aacggtagct ggcctggaga ggggggcgcg gacttgccag ccgggcgctt cggcgcggga
     3301 gtctgcggcg cgtcctgcgc cggggcgcag aggcagacgg ccagggcggg gagggcggtg
     3361 agaaaaatcg gcttcatggc ggaaaagggg cgcggggtgg aaatgcgggc ggggttattc
     3421 gaggccgagg taggcgtcgt cacggatgct gtaggtctgc atgatgcgcg ggtgcgcggt
     3481 ctcggccagg tcccgccgca gtacgagcgg gcggcctttt tccagcagct tgatgacgac
     3541 gaacttctcc tcctccttcg agagcgcctc gcgcacgtcc ttgagggtgc ggattttcat
     3601 gccgttgatc tcatccacca cgctgtgcgc gtaggggctg aggtgggtgt tcacctcgtc
     3661 ggccaggatg ctggtgagga tgaccggctc gggccgctcc acatagagct tctcggtgag
     3721 gaagttgtca aagatgtagc tggcggtcga gtcgttgatc tggtggctgt ccatcaggct
     3781 cttgtccatc ggctggaaga ccaggccggc atagacgatg tagcgcgggc gctggttgta
     3841 ctgctcgccc agccgcacat agggatcgaa gcgtttgagt gcgaccttcg tttcctgcct
     3901 cttgccgccg cgcaggatca cgagatcgag cacgtccccg gcgaacttgc gctccaccac
     3961 ctcgttcatg ttcaccatct ccccctccat gcggatgagg ccgttgttga agaccgggtt
     4021 gccgttgatg gagagcagca catcccctcg ctgcaaaatg ccgcccgcgc tgccggcagg
     4081 ctccacgtcg gcgatcatca cgcccgtgtt gtccacaagg cccagggcct tgcgctgcgc
     4141 gatgttctca atggcgaagt cactcaccgc gaagtccaca tagtggtcgt atttgccgtc
     4201 ctccacgtcc ttgaggaagc gctcgatgac cggcacaggg atgatgtagc ccacgttctg
     4261 cgccacccgc ccgctgaagc cctggaaagc cacgccgacc accttgccat cctgcatcac
     4321 cgggccgccc gagttgccgg ggttgatggc cgcgtccacc tggatggcca ggtgcgagtc
     4381 cacctgcgtg tggctgtagg ggcggaagtc aatgcgcgag accacgccgc gcgtcaccga
     4441 gatgcggtcc ccgccgatgg ggtagccaat ggcgatgacc tccgtgttca gcttcggcag
     4501 gccgccgatg gagagcggct tgagcttctc aaaaggctcg ccgtcctccg cctccaggat
     4561 cgccaaatcg cagtcgtggg cgatgtggac gatgcgcgcc gggtgcggcc gcgggtcgtt
     4621 ggaggtgcgc agcacgagct tggtggcgtt gctgaccaca tgagcgttgg tgagaaaacg
     4681 attcttgccg atgaggaagc ccgtgcccga gccgccggag ggctgccccg cgttccacgg
     4741 ctcgcggtag tcggggatga gcaccgagca gtcaatgttg accacgccct cccattcatc
     4801 cgtgctcaga tcaatcgcct ggccgcgcag cgcggctgga agaaaaacgg cggccagggc
     4861 ggccaggggg aggagggaaa atttcatgcc aatcacaaat tcaaagaacg caaccatacg
     4921 cggcggccct ccacgatgac aagagtttct ggagatttgc tgaaaatgcc ccgcatccgt
     4981 caagcacggc agcaagtctc gtccatgaaa cgctgaaata gctgcctggc gttttgcggc
     5041 gttttgcaca cgagcagcaa cttgaagatt gaactgaccc agtgatctcc tgccggcgat
     5101 ccgaacaaaa tggatgcagg caacggcctg aaggctgact gacgtgtcag caacgtcatg
     5161 cgctcgcctt cgcctgatcc gagaccttaa ttcagcctgc ctcggaagag ttgcttcagc
     5221 acatccccca agtcgagctt gtcctctctc tcataaggat cccggccacg atccggccgc
     5281 ggcggaaccg ggcctgatgg tatcggctga ggcaggacga gcggaggcag ctcaaagtgg
     5341 cgctcctgca ctccagaacc gcggctgcca taggaccggc catccatctc caggggccgg
     5401 agcatggggt tgcggtagcc aagccggccg atctcgctga gatgctggtc aatgtcccga
     5461 aaataaggaa acatgctgcg aatgaaaccc gcctgcaccg ccatcaactg cgagtctctg
     5521 gaggcatact ccgccatgtg aaaggagcgg taggcttcac ccagggggat gctggcattg
     5581 ccagtgtgcg cgcggatctg gctcgccagg cggctcacgt ccaactgcag gtccaccagc
     5641 gcgcgcatga gctgcccgtc cggactggcg gcggcaacct tgtagcgctt gcaataggag
     5701 acaaaggcct ggtgcagtgt gcgcgtggtg ctctgcaggc actccacctc ctggaggaga
     5761 taggactggc gctgcgcgac ggggtatctg tcatccggaa cgaaaacggg tcgctgcccc
     5821 agagcctggt tcccgatcgt caaggccaga gccgtcatga tggccgcctg aagtggagat
     5881 gagattttca tgaggggtca tggtatgaaa cccccgcccg ttcggcaatc accaattcca
     5941 aaacatgcac ccaaagggag acttcatgcc gggatgagcg ggctataata gctgccaggc
     6001 gtgaatggca ctcagttaag acaaggagcg gtgccgtagg ccgggtgtgt ccggcga
//
LOCUS       DAOMYJ010000271         6033 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Prosthecobacter sp. isolate AMR_MDS_5629
            NODE_4453_length_6033_cov_17.371696, whole genome shotgun sequence.
ACCESSION   DAOMYJ010000271 DAOMYJ010000000
VERSION     DAOMYJ010000271.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867850
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prosthecobacter sp. (wastewater metagenome)
  ORGANISM  Prosthecobacter sp.
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Verrucomicrobiaceae; Prosthecobacter.
REFERENCE   1  (bases 1 to 6033)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6033)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.30
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:34:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,742
            CDSs (total)                      :: 4,684
            Genes (coding)                    :: 4,676
            CDSs (with protein)               :: 4,676
            Genes (RNA)                       :: 58
            tRNAs                             :: 55
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6033
                     /organism="Prosthecobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4453_length_6033_cov_17.371696"
                     /isolate="AMR_MDS_5629"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1965333"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..778)
                     /locus_tag="PLP58_19750"
     CDS             complement(<1..778)
                     /locus_tag="PLP58_19750"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF012933.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000701 - purine-specific mismatch base
                     pair DNA N-glycosylase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HRJ10825.1"
                     /translation="MSAAAFARDLSAWFRKNGRDLPWRRTTDPYAILVSEVMLQQTQV
                     ATVLERGHYTRWMERFPDFATLAAAEENAVLQAWEGLGYYRRARNLLALARTVAHRHG
                     GVFPRDLESIRALPGIGPYTAGAVASFAHDTPAPLVDGNVARVLSRLDDDATPADSTA
                     GQKRLWQRAAELVLAASSPRVFNAALMELGQTLCRPGAPLCLSCPVKKHCRATDPAAL
                     PVKRARPAPTRVVERVFLLQENGSVLLEQERGPRRTGLWKL"
     gene            complement(794..2908)
                     /locus_tag="PLP58_19755"
     CDS             complement(794..2908)
                     /locus_tag="PLP58_19755"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HRJ10826.1"
                     /translation="MKFHTSHPALLAGLRLLAMCLTALPVKAADDPGDEYFRGYMMLK
                     DAEKMESEGNLNGAFSLYQQMAEIFDGVARAHPEWQPGMLATRRSLVEKAMNRVQAGL
                     IQPAAPAQAAPAAGLVIPQAGPAQPGTSGPLPSLTEALAQWEQTWRQRMTELETQNSQ
                     MQVDLGKWQQWYQWASGEITTARQRMTELERKSATLEAAIQAMQVEVAAGRAKASELD
                     ALTKEKLAIEVEYRKVSQRLNGAELAAREASQKLSDASLRISELETERNKALAERDAA
                     VKERDTAMTERDSAASARDAAVAERDKLKTQNLGMTAEIESLKKANPAAEEVKKLIAE
                     NERLRSDLESAQKQIVTLKADVSRKDQEVAALRSQVATLQGEIATLRQESAAYQTQVA
                     DLTLQLKNLSSEGGAMLTPELARENELLREIIMRQLRGQYRQQQAKELVLAELKKMEG
                     VSRDLLEQVQELETNRLALTPDEEKLFTDPAVREMLGQDGGIQGTLIARIPKNDGAAK
                     PGASPVDTLLNSANEAFAAQRFAEAAGLFEDALRADPQNTTALVGLGYARQRENKLDA
                     AAAALKKCLAIDPGHDLASFHLGVTHFKQQQWQDSMASFEKSLAKAPNNARARHYLGI
                     ISTKLNLLDRAEREFKTALAIDPAYGEAHFNLAVLYATWDPPQWDKAKAEYEQALKKG
                     VTPDEALEKLLNGSAAKSVSAR"
     gene            3090..4694
                     /locus_tag="PLP58_19760"
     CDS             3090..4694
                     /locus_tag="PLP58_19760"
                     /inference="COORDINATES: protein motif:HMM:NF012686.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ10827.1"
                     /translation="MRRKSRPLLFPALLLPATIHAAETGGTPAAHGLLWVDWLVVAVY
                     MALTLYIGWRCSRDEKTTGSYFIGSGKMNPVLIGVSLFATLLSTISYMAMPGESLGKG
                     PVFFSNYLAYPLVFLVVSFGLLPIYMRHKVHSAYELLEERLGASVRLLGAGLFLLLRL
                     VWMSLMIHMTSNAIAIMAGLGEEWVPWIAAASGLVAVTYTSMGGLRAVVITDFMQTLL
                     LYGGALLVIATVTVQLGGFSWFPTEWRDNWDRQVVFTWDWSERVTMAGSIISVFFWMV
                     ATMGGDQVSVQRFMATEDAGAARRALAVQLCCSVVVGLTLACVGFALLGYFEAFPERL
                     GAGLDLKGKADKIFPHFIAFHLPPVVSGLVVSGLFAAAMSSLDSGVNSITAVVTRDFL
                     DRAGIGPKDDAAHFRMARWMAFGIGAVVVAGSSVMKNVPGNFMAVTNKVVNFFTVPIF
                     CLFLFALVLRFVSPAGAWIGALCGVAAAGMVAFSGPIFVPGFDAATMNDPVSFQWMQP
                     ASFIAAMAGGILGSLVFPRKEASLKK"
     gene            4710..5459
                     /locus_tag="PLP58_19765"
     CDS             4710..5459
                     /locus_tag="PLP58_19765"
                     /inference="COORDINATES: protein motif:HMM:NF021507.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphodiester glycosidase family protein"
                     /protein_id="HRJ10828.1"
                     /translation="MRHLLFLITAIAAAHGIEPVKYEGALYHVRRIPPGGHSRLELRW
                     LDAAGQPLVHFGGLKKQLATEGKKIAFATNAGLYEKGPRPCGLTIIEGRELVPLQLGE
                     GFGNFYLKPNGVFFLDDKTGPGIMETTGYAALSLRPRLAQQSGPLLLRKGAIHPAFRA
                     DSPNKRQRSAVGIVAATKEVVFVMSDREDRVQGRVTFHQLSRFFLHIGCQDALYLDGD
                     ISEMMDTTTRLPADAAERPTNTFAGMFVLVE"
     gene            5596..6012
                     /locus_tag="PLP58_19770"
     CDS             5596..6012
                     /locus_tag="PLP58_19770"
                     /inference="COORDINATES: protein motif:HMM:NF012371.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HRJ10829.1"
                     /translation="MSPLTRRRFLATSTCALLARPAPAVETSPAWAAKLEAIRARYDL
                     PALGAAIVTAEGCQSLAVTGVRKKDAETPVTTADLWHLGSNTKAMTATLAALAVQEGK
                     LRWDSTLGELFPRQRDLKKSPLAKATLTHRRDDDRL"
BASE COUNT          893 a         1798 c         2128 g         1214 t
ORIGIN      
        1 gcagcttcca gaggccggtg cggcgcgggc cgcgctcctg ctcgagcagg acggagccgt
       61 tttcctggag gaggaaaaca cgctcgacca cccgggtggg ggcggggcgc gcgcgcttca
      121 ccggcagcgc cgccgggtcg gtggcgcggc agtgtttttt gacggggcat gacaggcaaa
      181 gcggcgcgcc aggccggcag agggtctggc cgagctccat gagcgcggcg ttgaagacgc
      241 gcggcgagga cgcggcgaga accagctccg cggcgcgctg ccagaggcgt ttttgcccgg
      301 cggtggagtc tgcgggtgtg gcgtcgtcgt cgagccggga gagcacgcgc gccacgttgc
      361 catccacgag cggcgccggc gtgtcgtggg cgaagctggc caccgcgccg gcggtgtagg
      421 ggccgatgcc gggcagtgcg cggatgcttt cgaggtcgcg gggaaagacg ccgccgtgac
      481 ggtgtgcgac ggtgcgggcc agggcgagca ggttgcgcgc gcggcggtag tagccaaggc
      541 cctcccatgc ctgaagcacg gcgttttcct ccgcggcggc gagcgtggcg aagtcgggga
      601 agcgctccat ccagcgcgtg taatggccgc gctccagcac ggtggccacc tgcgtctgct
      661 ggagcatgac ctcggaaacg aggatggcgt agggatcggt ggtgcgccgc cagggcaggt
      721 cacgcccgtt cttccgaaac caggcggaga gatcgcgggc aaatgcggcg gcggacacgg
      781 ttggccgggg tgctcagcgc gcggagacgg acttggcggc gctgccgttg agcagttttt
      841 ccagagcctc gtcgggggtg acgccttttt tgagcgcctg ctcgtactcg gccttggcct
      901 tgtcccactg gggcgggtcc caggtggcgt agaggacggc gaggttgaag tgcgcctcgc
      961 cataagcggg gtcaatggca agggcggttt tgaactcgcg ctcggcgcgg tcgagcaggt
     1021 tgagcttggt ggagatgatg ccgaggtagt ggcgggcgcg ggcgttgttc ggggctttgg
     1081 cgagggattt ttcaaaggag gccatggagt cctgccactg ctgctgtttg aaatgggtga
     1141 cgccgagatg gaaggaggca agatcgtggc cgggatcaat ggcgaggcat tttttcagcg
     1201 cggcggcggc ggcgtcgagc ttgttttccc gctggcgggc gtagccgagg ccgacgaggg
     1261 cggtggtgtt ttgcgggtcg gcgcgcaggg cgtcctcgaa gagtccggcg gcctcggcga
     1321 agcgctgcgc ggcaaaggcc tcgttggcgc tgttgagcag ggtgtccacc gggcttgcgc
     1381 cgggtttggc ggcaccgtcg tttttgggga tgcgggcgat gagggtgccc tggatgccgc
     1441 catcctggcc gagcatctcg cgcacggcgg ggtcggtgaa gagtttttcc tcatccgggg
     1501 tgagggccag gcggttggtc tcgagttcct gcacctgctc gagaagatcc ctggagactc
     1561 cctccatctt tttgagttcg gccaggacga gttccttggc ctgctgctgg cggtactggc
     1621 cgcggagctg gcgcatgatg atctcacgca gcagttcgtt ttccctggcc agctcggggg
     1681 tgagcatggc gccgccttcg gaggagaggt ttttcagttg gagggtgagg tccgccacct
     1741 gggtctggta ggcggcgctt tcctggcgca gggtggcgat ctccccctgc agggtggcga
     1801 cctgggagcg cagggcggcc acttcctggt ccttgcggga aacgtcggcc ttcagggtga
     1861 cgatctgctt ttgcgcgctt tccaggtcgc tgcgcaggcg ctcgttttcc gcgatgagct
     1921 tcttgacctc ctcggcggcg gggttggctt ttttgagcga ttcgatctcg gcggtcatgc
     1981 cgaggttctg ggttttgagc ttgtcgcgct cggcgacggc ggcatcccgc gcggaggcgg
     2041 cgctgtcacg ctctgtcatg gcggtgtcgc gctccttgac ggccgcgtcg cgctcggcca
     2101 gggccttgtt gcgctcggtc tccagctcgg agatgcgcag ggaggcgtcg gagagttttt
     2161 gcgaggcttc cctggcggcc agctccgcgc cgttgaggcg ctggctgact tttcgatact
     2221 cgacctcgat ggcgagtttt tccttggtga gggcgtcgag ttctgacgcc ttggccctgc
     2281 cggcggccac ctcgacctgc atggcctgga tggctgcctc aagggtggcg gacttccgct
     2341 ccagctcggt catgcgctgg cgcgcggtgg tgatctcgcc ggaggcccat tggtaccact
     2401 gctgccattt gccgaggtcc acctgcatct ggctgttttg agtttccagc tcggtcatgc
     2461 gctggcgcca ggtctgctcc cactgcgcca gcgcctcggt gaggctgggc aacggaccac
     2521 ttgtgcccgg ctgggctggt cccgcttgtg gaatgacaag ccctgcggcg ggagctgcct
     2581 gggccggcgc ggcgggctga atgagaccgg cctgcacgcg gttcatggcc ttttccacga
     2641 ggctccgccg tgtggcgagc atgcccggct gccattcggg atgcgcgcgg gcgactccgt
     2701 caaaaatctc cgccatttgc tggtagagtg aaaaggcgcc gttcagattt ccctcggact
     2761 ccattttttc cgcgtccttg agcatcatgt agccgcgaaa atattcgtcg cccggatcgt
     2821 cggcggcctt caccggcagg gcggtgaggc acatggccag caggcgcaaa ccggccagga
     2881 gggctggatg ggaggtgtgg aatttcatgc gggcgcgcag tctaagcgcc cggttccttt
     2941 ttgtaaacag cgccggaaac ggcctgccgg gcgccgggac atgggtgtgt ttgctccgct
     3001 ttgtcgaaaa gtcgccgtgc gggaggtgtt attttgtgac ctgtccgcca ggcttgaccc
     3061 ttcaaggcgc ggtgttcagg atgattttta tgagacgcaa atcccggccg cttctgttcc
     3121 cagccctcct gcttcccgcc acgatccatg cggctgagac cggcggcacc ccggcggcgc
     3181 atggactgct gtgggtggac tggctggtgg tggcggttta catggcgctg acgctttaca
     3241 tcggctggcg ctgctcgcgt gatgagaaaa cgacggggtc ttatttcatc ggttcgggaa
     3301 aaatgaatcc ggtgctcatc ggtgtgtcgc tctttgccac acttctgagc acgatttctt
     3361 acatggcgat gccgggagag agtcttggca aggggccggt gttcttttcc aactacctgg
     3421 cctaccccct ggtcttcctg gtcgtgtcct tcggactgct gccgatctac atgcgccaca
     3481 aggtccacag cgcctacgag ctgctggagg agcggctggg cgcgagcgtg cgcctgctgg
     3541 gcgcgggtct ttttctgctg ctgcggctgg tgtggatgtc gctcatgatc cacatgacct
     3601 cgaacgccat cgccatcatg gcggggctgg gggaggaatg ggtgccgtgg atcgcggcgg
     3661 caagcgggct ggtggcggtc acctacactt caatgggcgg actgcgcgcg gtggtgatca
     3721 cggatttcat gcagaccctc ctgctctatg gaggcgccct gctggtgatc gccacagtga
     3781 cggtgcagct gggcggcttt tcctggttcc ccaccgaatg gcgtgacaac tgggacaggc
     3841 aggtggtctt cacctgggac tggtcggagc gggtgaccat ggccgggtcc atcatttccg
     3901 tgtttttctg gatggtggcc acgatgggcg gcgaccaggt ctcggtgcag cgcttcatgg
     3961 ccaccgagga cgcgggcgcg gcgcggcgcg cgctggcggt gcagctctgt tgcagtgtgg
     4021 tggtgggtct cacactggct tgcgtcggct tcgcgctgct gggctacttc gaggcgtttc
     4081 ccgaaaggct cggcgctgga ctggacttga agggaaaagc cgacaaaatc ttcccgcact
     4141 tcatcgcctt tcatctgccg ccggtggttt ccggcctggt ggtctcgggt ctgttcgcgg
     4201 cggcaatgtc gagcctcgac tcgggtgtga actccatcac cgccgtggtg acgcgggatt
     4261 tcctcgaccg cgccggcata ggcccgaagg atgacgcggc gcacttccgc atggcgcgct
     4321 ggatggcctt cggcatcgga gccgtggtcg tggcgggcag ctccgtcatg aagaatgtgc
     4381 cgggcaattt catggccgtg acaaacaaag tggtgaactt ctttaccgtg ccgattttct
     4441 gtcttttcct tttcgcgctg gtgctgcgct ttgtctctcc cgccggtgcc tggatcggcg
     4501 cgctctgcgg tgtggctgcg gcgggcatgg tcgctttctc aggcccgatc ttcgtgcccg
     4561 gtttcgatgc tgcgacgatg aatgatccgg tcagttttca atggatgcag cccgcgtcat
     4621 tcatcgccgc catggctgga ggcattctgg gcagcctcgt ctttccgaga aaagaagcgt
     4681 cactcaaaaa atgatctccc cccactgaca tgcgccacct gttgtttctg atcacagcca
     4741 tcgccgcggc gcacggcatc gagccagtga aatacgaggg cgcgctctac catgtgcggc
     4801 gcatcccgcc aggcgggcac tcccggctgg aactgcgctg gctggatgcc gcaggccagc
     4861 cgctggtcca ttttggcggt ctgaaaaaac agctcgccac ggagggcaaa aagatcgcat
     4921 tcgccacgaa tgcggggctt tatgaaaaag gcccgaggcc ttgcgggctg acgatcatcg
     4981 aaggccgcga gctggtgccg cttcaactcg gggagggctt tgggaacttc tacctgaagc
     5041 cgaacggggt tttctttctc gatgacaaaa ccggaccggg catcatggag accaccgggt
     5101 atgccgcgct aagcctgcgc ccgagactcg cccagcagtc ggggcctttg ctcctgagga
     5161 agggcgccat ccaccctgca tttcgtgcgg actcgccgaa caagcgccag cgcagcgcgg
     5221 tgggcatcgt ggcggcaacg aaggaggtgg tctttgtcat gagcgaccgg gaggatcgtg
     5281 tgcaggggcg cgtgaccttc caccaactca gccgcttttt tctgcatatc ggctgccagg
     5341 acgcgctcta cctggatggt gacatctccg agatgatgga caccaccaca cgcctgcccg
     5401 ccgatgccgc cgagcgtccc acgaacacct tcgcgggcat gtttgtgctg gtggagtgag
     5461 tcctgctgga atgaagcctt caagtgccgc aggttggagt gaagccttca ggcgccacgg
     5521 gagttgactc cgctgcggga attcatggaa caagctaaag cttgaactcc aaccggcggc
     5581 gaggtgtaat gcggcatgag cccgctgacc cgccgccgtt tcctcgccac cagcacctgc
     5641 gccctgctcg cgcgcccagc cccggcggtg gagacaagcc ccgcctgggc ggcaaagctg
     5701 gaggccatcc gcgcccgcta cgacctgccc gcgctcggcg cggccatcgt cacggcggag
     5761 ggctgccaaa gcctggcggt gaccggagtg cggaagaagg acgccgagac cccggtgaca
     5821 accgccgacc tttggcatct cggctcgaat acgaaggcga tgaccgccac actcgccgcc
     5881 ctggctgttc aggagggcaa actgcggtgg gacagcacgc tgggggagct tttccccagg
     5941 cagcgggact tgaagaagtc cccgctcgcc aaggccaccc tcacccaccg ccgagacgac
     6001 gaccgccttt gacctctcct tcatcttcag gat
//
LOCUS       DAOMYJ010000272         6031 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Prosthecobacter sp. isolate AMR_MDS_5629
            NODE_4460_length_6031_cov_19.238621, whole genome shotgun sequence.
ACCESSION   DAOMYJ010000272 DAOMYJ010000000
VERSION     DAOMYJ010000272.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867850
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prosthecobacter sp. (wastewater metagenome)
  ORGANISM  Prosthecobacter sp.
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Verrucomicrobiaceae; Prosthecobacter.
REFERENCE   1  (bases 1 to 6031)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6031)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.30
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:34:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,742
            CDSs (total)                      :: 4,684
            Genes (coding)                    :: 4,676
            CDSs (with protein)               :: 4,676
            Genes (RNA)                       :: 58
            tRNAs                             :: 55
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6031
                     /organism="Prosthecobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4460_length_6031_cov_19.238621"
                     /isolate="AMR_MDS_5629"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1965333"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..686
                     /locus_tag="PLP58_19775"
     CDS             <1..686
                     /locus_tag="PLP58_19775"
                     /inference="COORDINATES: protein motif:HMM:NF021507.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phosphodiester glycosidase family protein"
                     /protein_id="HRJ10830.1"
                     /translation="AALRHGARLHVFEARKGAEAVRLSGVVFDDRHLALRIVDQPDEQ
                     AGGARIEKIARELDASACLNGGFFQRDFTPLGQFVSDGRTTGAFTRSSLVSGAVLVRG
                     GSPALVWNAEFTGAYGITQMLQAGPRLVDAGRPVGTLEKTRSARRSFIAHDGRHLWLA
                     GTAEDCTLAALAEILVTPGLLPGFTTRRALNLDGGGSTALCARLADGGEFHIRSWRTV
                     RNYLAIVAR"
     gene            complement(714..1472)
                     /locus_tag="PLP58_19780"
     CDS             complement(714..1472)
                     /locus_tag="PLP58_19780"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HRJ10831.1"
                     /translation="MKAFSLENKRALVTGSSQGIGAAIADGLAEAGALVARHGIQERP
                     AELPADAPYLQEDLLDPGAPARLIAAAGPLDILVCNAGGFFDLPVLEMNRERWERTFQ
                     LNVASTFFIAQAFARSCVEAGRAGCIVITSSTNGFQAEADSCAYDASKGALVMLTRSL
                     AVSLAPHGIRVNGIAPGLIRTPLTAQWMEPRAHDLLRHYENKILLGEVGRPWDCAGAV
                     VFLCSEAARYLTGEIIVIDGGLTVCQIGKMTAQP"
     gene            complement(1453..2427)
                     /locus_tag="PLP58_19785"
     CDS             complement(1453..2427)
                     /locus_tag="PLP58_19785"
                     /inference="COORDINATES: protein motif:HMM:NF016777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HRJ10832.1"
                     /translation="MLIDCHNHVGADLLFYLHGDFPYAQHLVAMMDEGGALGVDRWVV
                     FPFVSHAALDISGFLENEIREGGMQLATVPYALENLRLLREIHELFPERAPRLLPFVM
                     ADPMRNTAAQAVELRKLRARYRFHGIKMQTTILQADIKHLRDKGKVFVELAAEWDVPF
                     LIHSSVAESDLWAQARDILDIAEENPGVRFCLAHSCRFDKECLDRVNDLPNTWFDNSA
                     HCIHCEGVVKDMPFVAPKHRRFDSDYSDPARVIADLAAAYPGKFMWGSDSPFYSYAAG
                     INGRVVRLICTYEREIRALKSNPPDVVHRITDTNTRAWLKLADESLLS"
     gene            complement(2484..2939)
                     /locus_tag="PLP58_19790"
     CDS             complement(2484..2939)
                     /locus_tag="PLP58_19790"
                     /inference="COORDINATES: protein motif:HMM:NF012257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030551 - cyclic nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HRJ10833.1"
                     /translation="MSDTLNTPRLPGLGILQPLGDEDRDILSGYGEFRPVQAGQHLIE
                     EGMTQNGVYFVISGKFHASSTRGGHKVLLGAVETGETIGEINVLDPSVASATVTAREF
                     SQVWFIDSKSLETYINEYPGPAAWLLIGIGKTIARRLRAVNEKIASLYS"
     gene            3097..4944
                     /gene="htpG"
                     /locus_tag="PLP58_19795"
     CDS             3097..4944
                     /gene="htpG"
                     /locus_tag="PLP58_19795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009961553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone HtpG"
                     /protein_id="HRJ10834.1"
                     /translation="MSTPTTHEFQAEVRQVLDIVIHSLYTDREIFIRELVSNAADALE
                     KMRLTQLTEKEVFGAELPLEIRITTDETAGTITIADHGIGMTRAELVENLGTIAHSGS
                     KAFAAALKNAGKTSEASLIGQFGVGFYSAFMVADEVKVYTHSWRNDGEHLVWSSTGAG
                     TYSIDEAPDQARGCRIVIKLKEDAKEFAQAHRVRSILGKYSNFVGFPIQLNGERVNTV
                     EAIWLKSKDEVSEEQYEEFYKFTAHAFDKPGYRLHFQSEVPLAINALLFLPEQNMELF
                     GMGQMEPGVGLYCKKVLIDPHPKKLLPEWMRFVRGVMDSEDLPLNISRESMQDSALVR
                     KLGEVVTKRLLKFLDKEAQDDAKKYQEFYGKFSRFLKEGVATDFANREAIAKLLRFES
                     SLTGKGEVIGLADYAGRMKEDQKAIYYQVAPSRSTIESGPYVEAFKSKGYEVLYLYET
                     IDEYVVSSLREFEGKPLQAVNSPDVDLGDISSEGEALSEEETGKLCEWLKETLAAGVE
                     EVRAGKRLVSSPALAITPEGEMPPQMRQMMRAMKPDEVEAPKVILEVNPRHEIIKKLA
                     ALSASDAGAAKLVGEQVLDNALLSAGLLDDPQRIVARTEKIMARMLGGA"
     gene            5020..5757
                     /gene="dapB"
                     /locus_tag="PLP58_19800"
     CDS             5020..5757
                     /gene="dapB"
                     /locus_tag="PLP58_19800"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009960758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008839 - 4-hydroxy-tetrahydrodipicolinate
                     reductase [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HRJ10835.1"
                     /translation="MSELQLLVTGCKGRMGQAVINAAAAQGVAVGAGIDVGDALPEAL
                     AKCSCVIDFTSHHFSDELVAECLRQGRSMVIGTTGHTADELARIRAAAEKLPIVLASN
                     FSVGVNTLFWLTRKAAELLGPDFDLEVVEMHHRMKKDAPSGTARTLVEILAAARGLEY
                     EKDCRHGRHGDVGARTPAEIGVHALRGGDVVGDHTVIFANVGERVELTHKASSRDTFA
                     GGAVRAARWLADKPAGLYDMQDVLGLA"
BASE COUNT         1083 a         1900 c         2061 g          987 t
ORIGIN      
        1 ccgcggcttt gcggcatggc gcgcggctgc atgtcttcga ggcgcgcaag ggcgcggagg
       61 cggtgcggct gagcggcgtg gtctttgacg accggcatct cgcgctgcgg atcgtggacc
      121 agccggatga gcaggcgggc ggcgcgcgca ttgaaaaaat cgcccgcgag cttgacgcaa
      181 gcgcctgcct caacggcggc ttttttcagc gggacttcac accactcggc cagttcgtct
      241 cggatggccg gaccacgggc gccttcacgc gctccagcct cgtctccggc gcggtgctcg
      301 tgcgcggcgg ctctcccgcg ctggtgtgga atgccgagtt caccggcgcg tacggcatca
      361 cccagatgct ccaggccggg ccgcgcctcg tggatgccgg gcggcccgtc gggactttgg
      421 agaaaaccag gagcgcgcgg cgcagtttca tcgcgcatga cgggcggcac ctctggctgg
      481 ccggcaccgc ggaggattgc acgctcgccg ccctcgcgga aatcctcgtc acacccggcc
      541 tgctgcccgg attcacaacg cgccgcgcgc tgaacctcga cggcggcggc tcaaccgcgc
      601 tttgcgcgcg ccttgcggac ggcggcgagt tccacatccg aagctggcgc acggtgagga
      661 actacctcgc catcgtggcg cggtgagggg ggatcaaaag gaccgctccc ccttcaaggc
      721 tgcgctgtca ttttgccgat ctggcagacc gtcagcccgc cgtctatgac gatgatctcg
      781 ccggtgaggt aacgcgcggc ttcggagcac aggaacacca ccgcgcccgc gcaatcccac
      841 gggcggccga cctcgccgag gaggattttg ttttcgtaat ggcgcagcag gtcgtgcgcg
      901 cgcggctcca tccactgcgc ggtgagcggc gtgcggatga ggccgggggc gatgccgttg
      961 acgcggatgc catgcggcgc gagcgagacg gcaagcgagc gcgtgagcat gaccagcgcg
     1021 cccttgctgg cgtcgtaggc gcaggagtcc gcctcggcct ggaagccgtt ggtcgaggag
     1081 gtgatgacga tgcagcccgc gcgcccggcc tcgacgcagg agcgcgcgaa ggcctgcgcg
     1141 atgaagaagg tggaggccac gttgagctgg aaggtccgct cccagcgctc gcggttcatt
     1201 tccagcaccg gcaggtcaaa aaagccgccg gcgttgcaga ccaggatgtc gagcggaccg
     1261 gcggctgcga tcagacgcgc gggcgcgccg ggatcgagca ggtcctcctg gaggtaaggc
     1321 gcgtccgccg ggagttccgc gggcctttcc tggatgccgt gccgcgcgac caatgcgccc
     1381 gcttcagcga gtccgtccgc aatggccgcg ccgatgccct gggaggagcc ggtgacaagg
     1441 gcgcgcttgt tttcaagaga gaaggctttc atcggcgagc ttgagccagg cgcgggtgtt
     1501 ggtgtcggtg atgcggtgga cgacatcggg cgggttgctt ttcagcgccc ggatctcgcg
     1561 ctcgtaggtg cagatgaggc gcaccacccg gccgttgatc ccggcggcgt agctgtaaaa
     1621 gggcgagtcg ctgccccaca tgaacttgcc gggataggcg gcggcgaggt cggcgatgac
     1681 gcgcgcgggg tcgctgtagt cgctgtcaaa gcggcggtgc ttcggcgcca cgaagggcat
     1741 gtctttgacg acgccctcgc agtggatgca gtgggcggaa ttgtcgaacc aggtgttcgg
     1801 caggtcgttg acgcggtcca ggcattcctt gtcgaagcgg caagagtgcg ccaggcagaa
     1861 gcgcacgccc gggttctcct cggcgatgtc gaggatgtcc cgcgcctgcg cccacaggtc
     1921 gctctcggcg acgctggagt ggatgaggaa gggcacgtcc cactcggcgg cgagttccac
     1981 gaagactttg cccttgtcgc gcaggtgctt gatgtccgcc tgcaggatgg tcgtctgcat
     2041 tttgatgccg tgaaaacggt agcgggcgcg cagcttgcgc agctccacgg cctgcgcggc
     2101 ggtgttgcgc atcgggtccg ccatcacaaa gggcagcagc ctcggcgcgc gctccgggaa
     2161 cagctcgtgg atttcccgca gcaggcgcag gttctccagc gcgtagggca cggtggcgag
     2221 ctgcatgccg ccctcgcgga tctcgttttc caggaaaccg ctgatgtcca gggccgcgtg
     2281 gctgacaaag ggaaagacca cccagcggtc cacgcccagc gcgcctccct cgtccatcat
     2341 cgccaccaga tgctgcgcgt aggggaagtc gccgtgaaga taaaacagca ggtccgcgcc
     2401 gacgtggttg tggcagtcaa tgagcatggc gaatcatacc gcgcgggggc ggcgttgtta
     2461 tcagcggaat gcgcggcgcc ggctcaggag tagaggctgg cgatcttttc attcaccgcg
     2521 cgcaggcggc gggcgatggt cttgccgatg ccgatgagca gccatgcggc cgggccgggg
     2581 tattcattga tgtaggtttc caggctcttc gagtcaatga accacacctg gctgaactcg
     2641 cgcgcggtga cggtggcact ggccacggag gggtcgagca cgttgatctc gccgatggtc
     2701 tcgcccgtct caaccgcgcc gagcagcacc ttgtgcccgc cgcgcgtcga gctggcgtgg
     2761 aatttgccgg agatgacaaa ataaacgccg ttctgggtca tgccctcctc gatgagatgc
     2821 tggcccgcct gcacggggcg gaactcgccg tagccgctga ggatgtcgcg gtcctcgtcg
     2881 ccgaggggtt gcaggatgcc gaggccggga aggcgcggag tgttcagggt gtcgctcatg
     2941 atttttttga aatacccgcc tttcttagtg ggactcggtg ttcctggcaa gcctgtgaac
     3001 ggaaaagatg gcgtcgcatg ccgcgcaagc gggggcttga ccgcacccgg cggcccatta
     3061 ttctccgcgc ccttcaaccc cgaaaaaacc gcaaacatga gcactccgac cacccatgaa
     3121 ttccaggccg aagtgaggca ggtcctggac atcgtcatcc actccctcta caccgaccgc
     3181 gagattttca tccgcgaact ggtctccaac gccgccgacg cgctggaaaa gatgcgcctc
     3241 acccagctca ccgagaagga ggtcttcggc gccgaactgc ccctggaaat ccgcatcacc
     3301 actgacgaga ccgccggcac catcaccatc gccgaccacg gcatcggcat gacacgcgcc
     3361 gaactggtcg agaatctcgg caccatcgcc cactccggct cgaaggcctt cgccgccgct
     3421 ctcaaaaacg ccggcaagac cagtgaggca agccttatcg gccagtttgg cgtcggcttt
     3481 tacagcgcct tcatggtggc cgacgaggtc aaggtttaca cccactcgtg gcgcaacgac
     3541 ggcgagcatc tcgtctggtc cagcaccggc gcgggcacct actccataga tgaagcgccg
     3601 gatcaggcgc gcggctgccg catcgtcatc aagctcaagg aggacgccaa agagttcgcc
     3661 caggcgcacc gggtgaggag catccttggc aaatattcga acttcgtcgg ctttcccatc
     3721 cagctcaacg gcgagcgcgt caacacggtc gaggccatct ggctgaagag caaagacgag
     3781 gtcagcgagg agcagtacga ggagttctac aagttcaccg cccacgcctt tgacaagccc
     3841 ggctaccgcc tgcacttcca gtccgaagtg ccactggcca tcaacgcgct gcttttcctg
     3901 ccggagcaga acatggagct tttcggcatg ggccagatgg agcccggcgt cggcctctac
     3961 tgcaaaaagg tgctcattga tccgcacccg aaaaagctgc tgcccgagtg gatgcgcttc
     4021 gtgcgcggcg tcatggacag cgaggacctg ccgctgaaca tctcccgcga gtccatgcag
     4081 gacagcgcgc tcgtgcgcaa gctgggagag gtggtcacca agcgcctgct caagttcctc
     4141 gacaaggagg cgcaggacga cgcgaagaaa taccaggagt tttacggcaa gttcagccgc
     4201 ttcctcaaag aaggcgtggc caccgacttc gccaaccgcg aggcgatcgc caagctgctg
     4261 cgctttgaat ccagcctcac tggaaagggc gaggtcatcg gcctggccga ttacgcgggc
     4321 cgcatgaagg aggaccagaa ggccatttat taccaggtgg cccccagccg ctcgaccatc
     4381 gagagcggcc cgtatgtgga ggctttcaag agcaagggtt acgaggtgct ctacctctat
     4441 gagaccattg acgagtacgt cgtctccagc ctgcgcgagt tcgagggcaa gccgctccag
     4501 gccgtgaact cgcccgatgt ggacctgggc gacatctcca gcgaaggcga ggcgctcagc
     4561 gaggaagaaa ccggcaagct ctgcgaatgg ctgaaggaaa ccctggccgc gggtgtcgag
     4621 gaagtgcgcg ccggcaagcg cctggtgagc agccccgcgc tggccatcac acccgagggg
     4681 gaaatgcccc cgcagatgcg ccagatgatg cgcgccatga agcccgacga ggtcgaggcg
     4741 ccgaaagtca ttctggaggt caacccgcgc cacgagatca tcaaaaagct cgccgccctc
     4801 agcgccagcg atgccggggc cgcgaagctc gtcggcgagc aggtgctcga caacgccctg
     4861 ctctccgccg gcctccttga cgatccccag cgcatcgtcg cccgcaccga gaagatcatg
     4921 gcgcggatgc tcggcggcgc gtagccggcg gcatggccgc gcggcgcgga ctcttgcggg
     4981 gttgcggctg gcgcggaacg cgctatctcc acggctctta tgagcgaact ccaactactc
     5041 gtcaccggct gcaaaggccg catgggccag gccgtcatca atgccgccgc cgcgcagggc
     5101 gtggccgtgg gcgcgggcat tgatgtgggg gatgcgctgc cggaggcgct ggcgaagtgc
     5161 tcgtgcgtga ttgatttcac ctcccatcat ttcagcgacg agctggtggc ggagtgcctg
     5221 cggcagggga ggagcatggt gatcggcacc acgggccaca cggcggacga gctggcgcgc
     5281 atccgcgcgg cggcggagaa gctgcccatc gtcctcgcct cgaacttcag cgtgggggtg
     5341 aacacgctct tctggctgac gcgcaaggcg gcggagctgc tgggaccgga cttcgatctg
     5401 gaggtggtgg agatgcatca ccggatgaaa aaggacgcgc ccagcggcac cgcgcgcacg
     5461 ctggtggaga tcctcgccgc cgcgcgcggc ctcgaatatg aaaaggactg ccgccacggc
     5521 cgccacggtg atgtgggcgc gcgcacgccg gcggagatcg gcgtgcatgc cctgcgcggc
     5581 ggggatgtgg tgggcgacca taccgtgatc tttgccaatg tgggcgagcg cgtggagctg
     5641 acgcacaagg cgagcagccg cgacaccttc gccggaggcg cggtgcgcgc cgcgcgctgg
     5701 ctggcggaca agcccgccgg gctctatgac atgcaggatg tgctagggct ggcgtgaggc
     5761 ggtgccgttt ccgggtggat tcagggacgg attaagagac ggattaagag acggattatg
     5821 cctcgcgaca ccacagattg aaataagcgg atggagtcat cgagtgctgg agccttgaaa
     5881 atccaccact ccaggaatga attcacaaat cgtgattacg cgcggtataa gactggaatg
     5941 accacctttg gcatcgcctt cggctcgaac ctcggcgacc gcgccgcgaa cctgcggcgc
     6001 gggctggagc tgctgctggc gcggctgccg g
//
LOCUS       DAOMYJ010000273         6027 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Prosthecobacter sp. isolate AMR_MDS_5629
            NODE_4465_length_6027_cov_18.039183, whole genome shotgun sequence.
ACCESSION   DAOMYJ010000273 DAOMYJ010000000
VERSION     DAOMYJ010000273.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867850
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prosthecobacter sp. (wastewater metagenome)
  ORGANISM  Prosthecobacter sp.
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Verrucomicrobiaceae; Prosthecobacter.
REFERENCE   1  (bases 1 to 6027)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6027)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.30
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:34:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,742
            CDSs (total)                      :: 4,684
            Genes (coding)                    :: 4,676
            CDSs (with protein)               :: 4,676
            Genes (RNA)                       :: 58
            tRNAs                             :: 55
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6027
                     /organism="Prosthecobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4465_length_6027_cov_18.039183"
                     /isolate="AMR_MDS_5629"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1965333"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            272..1228
                     /locus_tag="PLP58_19805"
     CDS             272..1228
                     /locus_tag="PLP58_19805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ10836.1"
                     /translation="MKNILLPCLLVCLPLAAQQTEPPQTLTQEQMDNVMAQLKQLEDQ
                     IQNMRGGKLSTVMARLQAGLASKKDALNLYFECDKLVNAEIKEMDRTEARRRQEMMEK
                     RAKSEDPKEEGDFAQAVYLGLKYLVLTLEAHEARDEDFAKMAPKLQSYIQELVASAPL
                     LKGRSLGYLNGAVSDRNPIVEAFQLGRYLQREGWTTRSNDFGGMFSETLLPLAKKEKP
                     ETLPALWDSRISAEAAFNKESMTEPEYQRWLQDGLPVLKWERGKYLAEKGPSKITGLA
                     DMLAVIKEFPGHADAPKWLLEMRQAINGAAPSVSSANLPPAQ"
     gene            1232..1582
                     /locus_tag="PLP58_19810"
     CDS             1232..1582
                     /locus_tag="PLP58_19810"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013391.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HRJ10837.1"
                     /translation="MPAWTARFLRGFRHAFDGIAWALLCQRNMRFHAAATLAVTGLGM
                     VWNLELWKWCVLCLCAGLVWTAEIFNTAIELLCDLVQPERDERVRRIKDTAAGAVLAA
                     AIAAAVAGGIVFLC"
     gene            1650..3392
                     /locus_tag="PLP58_19815"
     CDS             1650..3392
                     /locus_tag="PLP58_19815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009964132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HRJ10838.1"
                     /translation="MSDLRPLTPALLSFVLLSGAARAAEARVDFTTQVKPVLESACTH
                     CHGADKDKGDFRLHTLEDMKKGNDNGPGLVPGDPAKSSIYTTLVLPEDDDMVMPPAKE
                     GLLDKSQIAVIKAWIEQGADWPAGLALEVTPRIDFAKNVQPILEQNCLSCHNPEKDKG
                     GWIASTKKEAFETGDNAPNILPFAAAKSALFTLAALPEDDDDLMPPAKSGGPLKKDEI
                     AILKSWIEQGAVWPEGVTLTAKEKKAGDTNNPDDIELVKKIHAFITATSKEKSEAEMK
                     PYDSKVPKTGAAYSMVAIKGGEFTMGSPDAEEGRIEDEGPQFKVKVKPFWMGKYEVTW
                     DEYEPFQLTAEGRNKDGSRKVWSPDDKPEDLISQPTPPYQPMDFGMGRNGYPAICMTQ
                     HAANKYCQWLSAQTGHFYRLPTEAEWEYAARAGTSTAYFFGDDASQLGEYAWYFENAP
                     NFQYSVIGKKKPNPWGLHDIYGNVCEWTLDQYAEGYQALAALGAGNKWVPSTTPYPHV
                     ARGGHYDDDPEMLRSAARRSSEPGWKQQDPQLPKSIWYLTDATWLGFRIVRPLEIPTV
                     EEMFAAWNNGVARE"
     gene            complement(3417..5825)
                     /locus_tag="PLP58_19820"
     CDS             complement(3417..5825)
                     /locus_tag="PLP58_19820"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional serine/threonine-protein
                     kinase/formylglycine-generating enzyme family protein"
                     /protein_id="HRJ10839.1"
                     /translation="MTPAEATPLPPPDALSTGTEPTLAPDLPAPPQRPGKRDGPPSHR
                     DDIVIQDYTLLKRVGSGAYGEVWLAQSVTGAYRAVKIVWREDFELTRTFHREFLGIQQ
                     FEPISRAHPCLVHILHVGWNEQRGFYYCVMELADDAEEGPNFEDIRAYVPRTLGTDMK
                     RHGRLDLTFCRDAGVYLADALHYMHNHGLTHRDIKPSNIIFVGGVCKLADIGLVAGFG
                     ERTFVGTEGFVPPEGPGTPQADIYSLGKVLYEMSSGKDRMEFPEIPSNLGDDEWPFWV
                     DLNRVICKACAPDLHERFQTAADFAEALQMVGVSRPESFPRRFARGVALTLAGSFLAG
                     AGLTAAKYQKEWAWVMPLPEKPEPPRPEPPRQGKPWQNARGQWFSFARDRHVADLPVE
                     VTTYRLFLEATLRAAEFSVVPYETPDKKIINCVMIPAADADAFCAWMTSRDRQAGRLD
                     MDHEYAWRPAANIARPPGSAARPEWQVIRAQIVRVLYGSVSLDSSPQGADVFNGQLRV
                     GRTPLEMPKVQAGEFDFEIRLPGYKTEHARGTLKEGQAVSFNARLRATGSVVFGKPWE
                     NSLGIQMMPLGRAMLAATETRRKDFAEFARSANLPPVEGRVLDEAPDLPVTIVTRAEA
                     AQFCRWLTDRERAKGLLEPDQEYRLPRDDEWSMAAYLPREKGATPAERNMGIEGIYPW
                     GFTSTPPAKTVNLLDKSADPSGKKSIAGYEDGQPGPAPVGSYRADTRGLFDLAGNVWE
                     WVAENWADNAAEGAVRGGAFTTMERTELLASFRRKTGVEERLPDVGFRILLGVNGLPA
                     RDEE"
BASE COUNT         1193 a         2129 c         1750 g          955 t
ORIGIN      
        1 gatggacggc aaagtcgtcc aacttgtcca gggccgcgaa aaagccctcg aaggccgcca
       61 gggcgggcgc caccccggcc agaaaggcca gcgtggagag gacgagcaga aattgcgcgg
      121 agggcatggt ggaaacgggc atctttgcgc gcgcgcgccg ggccgcaagg atgaaaggcc
      181 gcaaggcgga ccgaacctgc ccgccggtgt aaaaacatat ttccctcaag gaaacccgcg
      241 cctccgcgcg ttgttccaga acccacccgc catgaaaaac atcctgctcc cctgtctcct
      301 cgtctgcctg ccgctcgccg cccagcaaac agagccgccg caaaccctga cccaggaaca
      361 gatggacaac gtgatggccc agctcaagca gttggaggat cagattcaaa acatgcgcgg
      421 cggcaagctt tcgaccgtca tggccaggct ccaggccggt ctcgccagca agaaggacgc
      481 gctcaacctc tattttgaat gcgacaagct ggtcaacgcc gagatcaagg agatggacag
      541 gaccgaagcc cgccgccggc aggaaatgat ggagaagcgc gccaaaagcg aagaccccaa
      601 agaggagggc gactttgccc aggctgtgta cctgggcctc aagtacctcg tcctcaccct
      661 ggaggcgcac gaggccaggg atgaggactt tgccaaaatg gcccccaagc ttcagtcata
      721 catccaggag cttgtcgctt ccgctccgct tctcaaaggg cgctccctcg gttacttgaa
      781 cggcgctgtc agcgaccgca accccatcgt cgaagcgttt caactcggac gctatcttca
      841 gcgcgaggga tggaccacac ggtccaatga ttttggcggc atgttttccg aaaccctgct
      901 gccactggcc aaaaaagaaa agccggagac actacccgcc ctttgggaca gccgcatctc
      961 cgccgaggcg gccttcaaca aggagtcaat gaccgagccc gaataccagc gctggctgca
     1021 ggacggactg cctgtcttga aatgggagcg cggcaaatac ctcgctgaaa aaggcccctc
     1081 gaaaatcacc ggccttgccg acatgctcgc tgtcatcaag gaattccccg gccatgccga
     1141 cgcgcccaaa tggctgctgg aaatgcgcca ggccatcaac ggcgcggccc catccgtcag
     1201 cagcgccaat ctgccacccg cgcagtgagc catgcccgcc tggaccgcac gcttcctgcg
     1261 cggtttccgc cacgcctttg acggcattgc ctgggccttg ctctgccagc ggaacatgag
     1321 attccacgcc gctgccaccc tggcagtcac gggactcggc atggtctgga atctggagct
     1381 ttggaagtgg tgcgtcctct gcctttgcgc cggcctggtc tggacggcgg agatcttcaa
     1441 caccgccatc gagcttctct gcgacctcgt gcagccggag cgcgacgagc gcgtccgccg
     1501 catcaaggac accgccgccg gagccgtgct cgccgccgcc atcgcggcgg cggtcgcggg
     1561 cggcattgta ttcctgtgct gaccgcgccc cgcccgcaac ccgccgccgc agtggacaaa
     1621 aagcgcgcgc cgtgcaaagt gccgccccta tgtccgacct ccgccctctg acacctgccc
     1681 tcctgtcctt cgttttgctc tccggcgccg cccgcgccgc cgaggccagg gtggacttca
     1741 caacgcaggt gaagcccgtc cttgaatccg cctgcaccca ctgccacggg gccgacaagg
     1801 acaagggcga cttccgcctg cacaccctgg aggacatgaa aaaaggcaac gacaacggtc
     1861 ccggcctcgt gccgggagac cccgccaaat cctccatcta caccacgctc gtgctgccgg
     1921 aggacgacga catggtcatg ccgccggcca aggagggcct gctggacaaa tcccagatcg
     1981 ccgtcatcaa agcctggatc gagcagggcg ccgactggcc cgccggactc gcgctcgaag
     2041 tcacgccgcg cattgatttt gccaaaaatg tgcagcccat cctcgaacaa aactgccttt
     2101 cctgccacaa cccggagaag gacaagggcg gctggatcgc cagcacaaaa aaggaggcgt
     2161 ttgagacagg cgacaacgcc cccaacatcc tcccttttgc cgccgccaaa agcgccctgt
     2221 tcaccctggc cgcgctgccg gaggatgatg acgacttgat gccaccggcc aagagtggcg
     2281 gtccgctgaa aaaggacgag atcgccatcc tcaaaagctg gatcgagcag ggtgcggtgt
     2341 ggcccgaggg agtcaccctc accgccaagg aaaagaaggc cggggacaca aacaaccctg
     2401 acgacattga actggtcaaa aaaatccacg ccttcatcac cgccacttcc aaagagaaga
     2461 gcgaggccga gatgaagccc tacgacagca aagttccaaa gactggcgcg gcctattcca
     2521 tggtcgccat caaaggcggc gagttcacca tgggcagccc cgatgccgag gagggccgca
     2581 tagaggacga ggggccgcag ttcaaagtga aggtgaagcc cttctggatg ggcaaatacg
     2641 aggtcacctg ggacgaatac gagccattcc agctcactgc ggaaggccgc aacaaggatg
     2701 gcagccgcaa agtctggagc ccggatgaca agccggagga cctcatctcg cagccaacac
     2761 cgccctacca gcccatggac ttcggcatgg gccgcaatgg ctaccccgcc atctgcatga
     2821 cccagcacgc cgccaacaaa tactgccagt ggctcagcgc gcagacgggc cacttttacc
     2881 gcctgcccac cgaggccgag tgggagtatg ccgcccgcgc cggcaccagc accgcctact
     2941 tctttggcga cgacgcctcc cagctcggcg aatacgcctg gtatttcgaa aacgccccca
     3001 acttccaata cagcgtcatc ggcaaaaaga agcccaaccc ctggggcctc cacgacatct
     3061 acggcaacgt ctgcgaatgg acactcgacc agtacgcaga aggctaccaa gccctggccg
     3121 cgctcggcgc cggaaacaaa tgggtgccgt ccacaactcc ctacccgcat gtggcccgcg
     3181 gcggccatta tgacgacgat cctgaaatgc tgcgcagcgc cgcccgccgc tcctccgagc
     3241 cgggctggaa gcagcaggac ccgcagcttc ccaaatccat ctggtacctc actgacgcca
     3301 cctggctcgg cttccgcatc gtccggcccc tggagatacc cactgtcgag gaaatgtttg
     3361 ccgcatggaa caacggcgtg gccagggagt gacgctgcgg ctttgtctca agcctgtcac
     3421 tcctcatccc tggcaggcag tccattgacg ccaagaagga tgcggaatcc gacgtcaggg
     3481 agccgctcct ccacgccggt ttttctgcgg aatgaggcga gcagttccgt gcgctccatg
     3541 gtggtgaaag cgccgccgcg cacagcgccc tcggcggcat tgtccgccca gttttcggcc
     3601 acccattccc agacattgcc cgccaggtca aaaagcccgc gcgtgtcggc gcggtaactg
     3661 cccacggggg ccgggccggg ctggccgtcc tcataaccgg cgatgctttt tttgccggac
     3721 gggtccgccg acttgtcgag caaattgacc gtcttcgcgg gcggcgtgga ggtgaagccc
     3781 caggggtaga tgccttcgat gcccatgttc ctctcggcgg gtgtggcgcc cttttcacgc
     3841 ggcagatagg ccgccatgct ccactcgtca tctcgcggca ggcggtactc ctggtcaggc
     3901 tccagcagcc ccttggcacg ctcgcggtcc gtcagccagc ggcagaactg cgcagcctcg
     3961 gcgcgggtga cgatggtgac cggcaggtcg ggggcctcat ccaaaacacg cccttcaacc
     4021 ggcggcagat tcgcgctgcg cgcgaactcc gcgaagtcct ttcttcgcgt ctccgtggcc
     4081 gcgagcatcg cccggcccag cggcatcatc tgaatgccga ggctgttttc ccagggcttg
     4141 ccgaagacca cggagccggt cgcgcgcagg cgcgcattga agctcaccgc ctggccttcc
     4201 ttcagcgtgc cgcgcgcgtg ctcggtcttg tagccgggca ggcggatttc gaaatcaaac
     4261 tcgcccgcct gcaccttcgg catctccagc ggcgtccggc ccacgcgcaa ctggccgttg
     4321 aaaacatccg cgccctgggg cgagctgtcg aggctgacgc ttccgtaaag cacccgcacg
     4381 atctgcgcgc ggatcacctg ccactcggga cgcgccgccg agcccggcgg ccgggcgatg
     4441 ttcgccgcag ggcgccaggc atactcatgg tccatgtcca gccggcccgc ctgccggtcc
     4501 cggctcgtca tccacgcgca aaaagcatcc gcgtccgccg ccgggatcat gacgcagttg
     4561 atgatcttct tgtccggcgt ctcgtagggc accacgctga actccgccgc gcgcagcgtc
     4621 gcctccagaa acaggcggta ggtcgtcacc tccaccggca ggtcagccac atgcctgtcg
     4681 cgggcgaagc tgaaccactg cccgcgcgca ttctgccagg gcttcccctg cctgggcggc
     4741 tccgggcgcg gcggctcggg cttctccggc aggggcatca cccacgccca ttccttttga
     4801 tacttagccg ccgtcagccc ggccccggcg agaaaggagc ccgccaaagt cagcgccacc
     4861 ccgcgcgcga acctgcgcgg aaagctctcc ggccggctca cccccaccat ttgcagggcc
     4921 tcggcaaagt ccgccgccgt ctggaagcgc tcgtgcaaat ccggcgcgca ggccttgcag
     4981 atgacgcggt tgaggtccac ccagaacggc cactcgtcat cacccaggtt cgaagggatc
     5041 tccggaaact ccatccggtc cttcccgctg ctcatctcat agagcacctt gcccaggctg
     5101 tagatgtccg cctgtggcgt gcccggcccc tcgggcggca caaagccctc cgtgcccaca
     5161 aacgtgcgct cgccaaaacc cgccaccagc ccgatgtccg ccagcttgca cacaccgccc
     5221 acgaaaataa tgttcgatgg cttgatgtcc cggtgcgtaa ggccgtggtt gtgcatgtaa
     5281 tgcagcgcgt ccgccaggta aacccccgca tcgcggcaga aagtcaggtc aagccgccca
     5341 tgccgcttca tgtccgtgcc cagcgtgcgc ggcacatagg ctcggatgtc ctcgaagttc
     5401 ggcccttcct ccgcgtcatc cgccagctcc atcacgcagt aataaaaacc gcgctgctcg
     5461 ttccacccca cgtgcaggat gtggaccagg cagggatgcg cgcgcgagat cggctcgaac
     5521 tgctgaatgc cgaggaactc gcggtgaaac gtccgcgtca gctcgaagtc ctcccgccac
     5581 acgatcttca ccgcccggta ggccccggtc acactctgcg ccagccacac ctccccataa
     5641 gccccggagc ccacccgctt cagcagcgtg tagtcctgga tcacaatgtc atcccggtgc
     5701 gagggcggcc cgtcgcgctt gcccgggcgc tgcggcggcg ccggcaggtc cggagccagg
     5761 gtcggctccg tcccggtgga cagcgcgtcc ggcggcggca atggcgtggc ttcagcaggc
     5821 gtcatggttg accgcaaaac taagcgccgc gcgcgccgga cacaaccctt tagccgtctt
     5881 tcacacaacc ccggcagaca cccaaccgcg catcatcacc ccgccgctcg gagaccgcga
     5941 attcgtcttt caaaccgtgc ctttcagaaa attgcttgtg caaaaattac ccgttcctta
     6001 gccttcacct gcccccggat aaagcca
//
LOCUS       DAOMYJ010000274         6001 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Prosthecobacter sp. isolate AMR_MDS_5629
            NODE_4500_length_6001_cov_21.243189, whole genome shotgun sequence.
ACCESSION   DAOMYJ010000274 DAOMYJ010000000
VERSION     DAOMYJ010000274.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867850
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prosthecobacter sp. (wastewater metagenome)
  ORGANISM  Prosthecobacter sp.
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Verrucomicrobiaceae; Prosthecobacter.
REFERENCE   1  (bases 1 to 6001)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6001)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.30
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:34:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,742
            CDSs (total)                      :: 4,684
            Genes (coding)                    :: 4,676
            CDSs (with protein)               :: 4,676
            Genes (RNA)                       :: 58
            tRNAs                             :: 55
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6001
                     /organism="Prosthecobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4500_length_6001_cov_21.243189"
                     /isolate="AMR_MDS_5629"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1965333"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..677
                     /locus_tag="PLP58_19825"
     CDS             <1..677
                     /locus_tag="PLP58_19825"
                     /inference="COORDINATES: protein motif:HMM:NF024829.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="HlyD family efflux transporter periplasmic
                     adaptor subunit"
                     /protein_id="HRJ10840.1"
                     /translation="ARVRATEDSLARERRDLERTRIRTPFAGRLRATHTELGSYLTPG
                     ARVADFDSTGRYEIRLPVSLDDLAFLEAQTGADALLKASVAGQDLVWKGTVARTEGEV
                     ERASRSVYLVVEIEEDSRPENAFLKPGIFLRAEVAGRVAKGVFQIPRRAFLDEARLVV
                     VGPGDKLEIRSVKTLRGGRDSVLVSEGLQEGERVCLTSLPAPMNGMEVRVLESGQGSD
                     AQAAKF"
     gene            693..3860
                     /locus_tag="PLP58_19830"
     CDS             693..3860
                     /locus_tag="PLP58_19830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018970679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HRJ10841.1"
                     /translation="MEKAIYWFSKNHVAANFLMLAVMIIGFTSWGKLKKEIFPETSVD
                     VVLVRIPYPNATPEEVERGVCVPVEEAVEDVEGVDRIKSTAGQGMGSVVIEVKSGYKV
                     RDVMDDVKTRVDAITNFPEEAETPTLEEMILNAEVISVAIYADTDEASLLEMSNRLRD
                     ELQGLPEITKVTLSNNRPYEIGIEVSEDTLRAYGLTFDQVANAVRASSLDLPAGSVRT
                     EAGEVLVRTEARRYRADEFARITVVTRQDGSQVKLGEIADIVDGFEEIDLESRFNGKP
                     CMLLRVFRTGNEDTLKVAAAVKKFIAEDAPLLFPEGVSFEIWKDDSKYLSGRLDLLGR
                     NAIFGFVLVLIVLSLFLRPVLAFLVALGIPASFMGALMLMPWLGVSINMISLFAFILV
                     LGIVVDDAIIVGENVYERISRGEDPRHAAPAGTHEVGVVVIFGVLTTMMAFTPMLGLS
                     GVSGKIWPNIPLVVIPTLAWSLLQSKFVLPAHLALLRRHDPNKRTLSDKLLGGVDRSL
                     KAFIERVYQPVLNLAMRWRYVTTCLFLALLVTVGGLVGTGWIKFNFFPDVEADVVIAR
                     LRLADGVAFETTRDAVRKIEAASNRLNQEYLEKHGASVIRNTLASAGVQPFLNGFEAL
                     AGAPKGDHLGEVTIELIDGADRQMSGIEIASRWRELTGPIPGALELAFQTQGAGGGNA
                     IDLELTGTDIVELEKASEFVKTALADYQGVIDIADNNNPGQRELKLGIKPAGEILGLR
                     LMDVSRQVRQAFYGDETQRLQRGRDEVKVMVRYPEAERRSLQNLTEMKIRAADGSEIP
                     FHEVAEVKMGRSASAIRRADRRRAISVTADIDKAVPGANANEVVASLNAEVLPKMKER
                     FPGVSYGFEGEQKDQRQSVQEIGQKALLALLGIYVLLAIPLRSYFQPLIVMSVIPFGL
                     VGAALGHVGMGLNLSIMSMCGIVALAGIVVNDSLVMVEFVNRERALGHDSHDAAIKAG
                     MRRFRPILLTSVTTFVGIMPMVFETDLQARFLIPMAVSLGFGILFATFITLLLVPVIY
                     LIMEDTGRLLRRAAASQPPLPGFGI"
     gene            complement(4095..>6001)
                     /locus_tag="PLP58_19835"
     CDS             complement(4095..>6001)
                     /locus_tag="PLP58_19835"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HRJ10842.1"
                     /translation="VLLALEVLAHAAFEEAAPGIHVWKDTCNVHVIQSGDAALLINLG
                     DGSVLDHLEKIGVKRVEWVILTDHHREVCQGAPKLDRTVTRVAASEIEKEILEAPLSF
                     RKWHPRLGDKHSVHGASYVRPPADPVRVDHVLADGGVFEWRGLELACAGTPGHSPGGM
                     SFVLRRDGRASAFIGGVMHDGAKMTNWFDTEWDYGFGKGLDALITSVQKLAALDIGTA
                     FASQGPVIHDAAAQFAAYEKKLADFRPDYLRGYPVNHLSKRGPHPATKPTKANHIVEV
                     TPHLYMFGPEMAGKNFAILIADSGHALLLDCGLFPKLVLERIISDMKEFLGLKQIDAC
                     WISHSHGDHFTLFPALQDHGVKFWTMDTIADKCENPRFYDYPAMIGAYNAGFEKAKID
                     RVLKAGDVIEWEGYQLHIDWMPGQTEFGNALWLMLDGKKVVFTGDNLFGDPSDPAQNG
                     HECVNARNSAIIEEGYLVAAKYLQKLQPDIIMGAHGVLMTEPKAFIERYHDWALRAIR
                     KYKDLLPDKDYEYGYDPYWVSAYPYRVDVAQPAEVSITVRNFRDTPQKHRIVLQLPPG
                     VRAEPAVLEGSLPAKSRASFPVKLAADAAVIPSGVLMIPMDITLDGKRHGQAFDFIVQ
                     GREPGEAGGK"
BASE COUNT         1037 a         1969 c         1907 g         1088 t
ORIGIN      
        1 aggccagggt gcgcgccacg gaggactcgc tggcacgcga gcggcgcgac ctggagcgca
       61 cgcggattcg cacgcccttc gcggggcggc tgcgcgccac gcacacggag ctgggctcct
      121 acctcacgcc cggagcgcgc gtggcggact ttgactcgac ggggcggtat gagatccgcc
      181 tgcccgtctc cctcgacgat ctcgcctttc tcgaagccca gaccggcgcg gacgccctgc
      241 tcaaggccag cgtcgccggc caggacctcg tctggaaggg cacggtggcg cgcacggagg
      301 gcgaggtcga gcgcgccagc cgctccgtgt atctcgtggt ggaaatcgag gaggacagca
      361 ggccggagaa cgccttcctc aagccgggca tcttcctccg cgcggaggtc gccggccgcg
      421 tggcgaaggg tgtcttccag atcccgcgcc gcgcctttct cgatgaggcg cggcttgtcg
      481 tcgtcggccc cggtgacaag ctggagatcc gctcggtgaa gacgctgcgc ggcggcaggg
      541 actccgtgct cgtctcagag gggctgcagg aaggcgagcg cgtctgcctc accagcctgc
      601 ccgcgccgat gaacggcatg gaggtgcgcg tgctggagtc cggccagggt tcggacgcgc
      661 aggcggcgaa gttttaaccc gcagatcccg ccatggaaaa agccatctat tggttctcga
      721 aaaaccacgt cgccgccaac ttcctgatgc tggccgtgat gatcatcggc ttcacctcct
      781 gggggaagct caaaaaggaa atcttccccg agacctccgt ggacgtggtg ctcgtgcgca
      841 tcccctaccc caacgccacg ccggaggagg tggagcgcgg cgtgtgcgtg ccggtggagg
      901 aggcggtgga ggatgtcgag ggcgtggacc gcatcaaatc caccgccggc cagggcatgg
      961 gctccgtggt catcgaggtg aagagcggct acaaggtgcg ggacgtgatg gacgacgtga
     1021 agacgcgcgt ggacgccatc accaacttcc ccgaggaggc cgagacgccg acgctcgagg
     1081 agatgatcct gaatgccgag gtcatcagcg tggccatcta cgcggacacg gacgaggcga
     1141 gcctgctgga gatgagcaac cgcctgcgcg acgaactcca aggcctgccg gagatcacca
     1201 aggtcacgct ctccaacaac cgcccgtatg agatcggcat cgaggtctcc gaggacactt
     1261 tgcgcgctta cgggctgacc tttgaccagg tggccaacgc cgtgcgtgcc agctcgctcg
     1321 acctgccggc cggctccgtg cgcacggagg cgggggaggt gctcgtgcgc accgaggcgc
     1381 ggcgctaccg cgcggacgag ttcgccagga tcactgtcgt cacccgccag gacggcagcc
     1441 aggtcaagtt gggcgagatc gcggacatcg tggacggctt tgaggagata gacctcgagt
     1501 cccgcttcaa tggcaagccc tgcatgctgc tgcgcgtctt ccgcacgggc aatgaggaca
     1561 cgctgaaggt ggcggcggcg gtgaaaaaat tcatcgccga ggacgcgccg cttctctttc
     1621 cagagggcgt gagttttgag atctggaagg acgactcgaa gtacctctcc ggccgcctcg
     1681 acctgctcgg gcgcaacgcc atttttggtt ttgtcctcgt gctgatcgtc ctctcccttt
     1741 tcctgcggcc cgtgttggcc ttcctggtgg cgctgggcat ccccgcctcc ttcatgggcg
     1801 cgctcatgct catgccctgg ctgggcgtgt ccatcaacat gatctccctc ttcgccttca
     1861 tcctggtgct cggcatcgta gtggacgatg ccatcatcgt cggcgaaaac gtctatgagc
     1921 gcatcagccg gggcgaggac ccgcggcacg ccgcgcccgc cggcacgcac gaggtgggcg
     1981 tcgtcgtcat cttcggcgtg ctcaccacca tgatggcctt cacgcccatg ctgggcctca
     2041 gcggcgtgag cgggaaaatc tggccgaaca tcccgctcgt ggtcatcccc acgctcgcgt
     2101 ggtccctcct ccagtcgaag ttcgtcctgc ccgcgcacct ggccctgctg cgccggcatg
     2161 atcccaacaa gcgcacgctt tccgacaagc tgctcggcgg cgtggaccgc tcgctgaagg
     2221 cgttcatcga gcgcgtttac cagcccgtgc tgaatctcgc aatgcgctgg cgctacgtca
     2281 ccacctgcct cttcctggcg ttgctcgtca ctgtcggcgg actggtcggc accggctgga
     2341 tcaagttcaa cttcttcccc gatgtcgagg ccgatgtcgt catcgcccgg ctgcgtctcg
     2401 ccgatggcgt ggcctttgaa accacacgtg acgccgtgcg caagatcgag gccgcctcca
     2461 accggctgaa ccaggagtat ctcgaaaagc acggcgcctc cgtcatccgc aacacgctcg
     2521 ccagcgccgg tgtgcagccc tttctcaacg gcttcgaggc cctcgccggc gcgcccaagg
     2581 gagaccacct cggcgaggtg accatcgaac tcattgacgg cgcggaccgg caaatgagcg
     2641 gcatcgagat cgcctcccgc tggcgcgagc tcaccggccc catccccggc gcgctcgagc
     2701 tggccttcca gacccagggc gcgggcggtg gcaatgccat tgatctggaa ctgaccggca
     2761 ccgacatcgt cgagctggaa aaagcctccg agtttgtgaa gaccgccctc gccgactacc
     2821 agggcgtcat tgacatcgcc gacaacaaca accccggcca gcgcgagctg aagctcggca
     2881 tcaagcccgc cggcgagatc ctcggcctgc gcctcatgga tgtctcccgc caggtgcgcc
     2941 aggcctttta tggcgacgag acccagcgcc tccagcgcgg gcgggacgag gtgaaggtca
     3001 tggtccgcta ccccgaggcc gagcgccgct ccctgcaaaa cctcaccgaa atgaaaatcc
     3061 gcgccgccga cggcagcgag attccttttc atgaggtggc cgaggtcaag atgggccgca
     3121 gcgcctccgc catccgccgc gccgaccgcc gccgcgccat cagcgtcacg gcggacatag
     3181 acaaggccgt gcctggggcg aacgccaacg aggtcgtcgc cagcctcaac gccgaggtgc
     3241 tgccaaagat gaaggagcgc ttccccggag tcagctacgg cttcgaggga gaacagaagg
     3301 accagcgcca gtccgtgcag gagatcggtc aaaaagccct gctcgccctg ctcggcatct
     3361 acgtgctgct cgccatcccg ctgcgctcgt atttccagcc gctcatcgtc atgagcgtca
     3421 tccccttcgg ccttgtaggc gcggcgctcg gccatgtcgg catggggctg aacctcagca
     3481 tcatgtccat gtgcggcatc gtcgccctgg ctggcattgt ggtgaatgac agcctcgtca
     3541 tggtcgagtt cgtcaaccgt gagcgcgccc tgggccatga ctcccacgat gccgccatca
     3601 aagccggcat gagacgcttc cgccccattt tgctcacctc cgtcaccacc ttcgtcggca
     3661 tcatgcccat ggtcttcgag accgatctgc aggcgcgctt tttgatcccc atggctgtct
     3721 ccctcggctt cggcatcctc ttcgccacct tcatcacgct gctcctggtg cccgtgattt
     3781 atctcatcat ggaggacacc gggcggctcc tgcgccgcgc cgcagcctcg cagccgccgt
     3841 tgccagggtt tgggatttag tagaaacgga cgtccatctc gccacgccac gcctttgcaa
     3901 gcgcagccac gcggcacgat gtggccagcg gcggttacct tcgcagccac agccaggatg
     3961 ccggttagct tgagaaagct gtcactgttg gagatgtcct gcctttgccc gcgcagagcg
     4021 atcacaagaa gatttggccg tcactgccga agacgggtga gccaccgccc tgcagcacca
     4081 tgtccaaggg tggattattt tccgccggct tcaccgggct cgcggccctg gacgatgaag
     4141 tcgaaggcct gtccgtggcg tttgccatca agggtgatgt ccatcgggat catgagcacg
     4201 ccagacggga tgaccgcagc gtcggcggca agcttcacag gaaagctggc gcgggatttg
     4261 gcgggcaggc tgccctccag aactgcgggc tcggcgcgca caccgggcgg tagttgcaga
     4321 acgatgcggt gcttttgcgg cgtgtcgcgg aagttgcgca cggtgatgct aacctcggcc
     4381 ggttgcgcga catcgacacg atacggatac gcgctgaccc aatacgggtc gtagccgtat
     4441 tcatagtcct tgtcgggcag cagatccttg tatttccgga tcgcccgcag cgcccagtcg
     4501 tggtagcgct cgatgaaggc cttcggctcg gtcatgagca cgccatgcgc acccatgatg
     4561 atgtccggct ggagcttttg cagatacttc gccgccacga gataaccttc ctcaatgatg
     4621 gcgctgttgc gcgcgttcac gcactcgtgg ccgttttgcg cgggatcgct gggatcaccg
     4681 aacaggttgt caccggtgaa aacgaccttt ttgccatcga gcatgagcca cagcgcattt
     4741 ccaaactccg tctggccggg catccagtcg atgtgaagct gataaccttc ccattcgatc
     4801 acgtcgccgg ctttgagtac acggtcgatc ttcgccttct cgaaaccggc gttgtaggcg
     4861 ccgatcatcg ccgggtagtc gtagaagcgc gggttctcgc atttgtcggc gatggtgtcc
     4921 atcgtccaga acttcacgcc gtggtcctgc agcgcgggga agagcgtgaa gtggtcgcca
     4981 tgcgagtggc tgatccagca ggcgtcgatc tgcttgaggc cgaggaactc cttcatatcg
     5041 ctgatgatgc gctcgagcac gagtttcggg aacaagccgc aatccagcag cagggcatgg
     5101 ccgctgtcag cgatgaggat ggcgaagttc ttccccgcca tctccgggcc gaacatgtaa
     5161 aggtgcggcg tcacctcgac gatgtgattc gccttcgtcg gcttcgtggc gggatgcggg
     5221 ccgcgcttgg agaggtggtt caccggatag ccgcgcaggt agtcggggcg gaagtcggcg
     5281 agctttttct cataagccgc gaactgcgcc gccgcgtcat ggatgaccgg cccctgcgac
     5341 gcgaaggcgg tcccgatgtc aagcgcggcg agcttttgca ccgaggtgat gagcgcgtcg
     5401 agacccttgc caaaaccgta gtcccactcg gtgtcgaacc agtttgtcat cttcgcgccg
     5461 tcgtgcatga cgccgccgat gaaggccgag gcgcggccgt cacgcctcaa gacgaagctc
     5521 atgccaccgg gggaatgacc cggtgtgccc gcgcaggcga gttcaaggcc gcgccattcg
     5581 aagacgccgc cgtcagcgag gacgtggtcc acccgcacag gatctgcggg cgggcggaca
     5641 tagctggcgc catgcacgct gtgcttgtca ccgagacgcg gatgccattt gcggaaggag
     5701 agaggggctt cgaggatttc cttctcgatc tccgatgccg ccaccctcgt gaccgtccgg
     5761 tccagcttcg gcgcaccctg gcagacttcc cggtggtggt cggtgaggat cacccactcc
     5821 acacgcttga cgccaatttt ctcaagatgg tcaagcacac tgccgtcgcc cagattgatg
     5881 agcagcgcgg cgtccccgct ttggatcaca tggacgttgc aggtgtcctt ccagacatga
     5941 atgccgggcg ccgcttcttc aaatgcggcg tgcgccagta cttcgagggc cagcagaacg
     6001 g
//
LOCUS       DAOMYJ010000275         5862 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Prosthecobacter sp. isolate AMR_MDS_5629
            NODE_4654_length_5862_cov_21.804202, whole genome shotgun sequence.
ACCESSION   DAOMYJ010000275 DAOMYJ010000000
VERSION     DAOMYJ010000275.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867850
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prosthecobacter sp. (wastewater metagenome)
  ORGANISM  Prosthecobacter sp.
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Verrucomicrobiaceae; Prosthecobacter.
REFERENCE   1  (bases 1 to 5862)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5862)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.30
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:34:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,742
            CDSs (total)                      :: 4,684
            Genes (coding)                    :: 4,676
            CDSs (with protein)               :: 4,676
            Genes (RNA)                       :: 58
            tRNAs                             :: 55
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5862
                     /organism="Prosthecobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4654_length_5862_cov_21.804202"
                     /isolate="AMR_MDS_5629"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1965333"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            179..2242
                     /locus_tag="PLP58_19840"
     CDS             179..2242
                     /locus_tag="PLP58_19840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ10843.1"
                     /translation="MKTTMPRPIEGIAAHVPRMALLLLACSVSALQAQEMVTRSFDVP
                     TKFPRLVGEIFPKPESGESQPYDPFSPDNPPGLRPQEFSKSTLQILRDAGLPFPEGSA
                     AFHDATHHVLTVRNTPANMKIVEQVIATLNEELPVTLSIRADLVEAPGSELRRIAALD
                     FEKAAPALAALLKQAFVPGSNVRMVRTALVESQSGMRSDILSGFEDWEHSGIEAGENG
                     HVIQIEKTARGFHFETDAYGSNSALHGRGIDLNFAVSHHPLLNQINEVEPQERMPFQH
                     GRDSISTSIQPRDGLPCLAGLWDAPSQNQDVLQAVVITVKTIIHKPLRTKWNNAFRPN
                     LEEPIAKSVQHVFDLPKDAWTLPARFGLGENKEIADDLRQADIQLPEDARVSLETPHK
                     LSVQADMETMEVIQRWLVEQHRGQATSLDMTLEIVRGPASLLRPMLEDIGDSGDDTSL
                     WSQAHEAVRRGEARIISTLHARVDSKHEVQLTAGNGERRLNEVGWTQGRDQHAWLATE
                     EGFTGVKLKLLPTLGEDGQTMAAECKLEWNPAPAIPGRMAIPEASDGGIELPHIRERR
                     MELSSHMVLMDGATKLIGAWNPESEEAGDAGSIMELAFMKVHFARHPGGAPVDATTRP
                     LPPGVEPPPPPKPGMETRVFRVSPSFLSSAKLPSDESDPSLPVSSAKKIFSDHGFPLP
                     EGSTN"
     gene            2289..3536
                     /locus_tag="PLP58_19845"
     CDS             2289..3536
                     /locus_tag="PLP58_19845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HRJ10844.1"
                     /translation="MSTLPSLNDHYSLLLGLDKHWEVERVDLQLAAKRVRIDLRHVRG
                     AALSCPQCQNACVMFDHAPEREWRHLDTMQFETVLRARVPRCKCEDCGVKTIAVPWAG
                     KHSPFTWLFEAFAIAVLQSAGSTSDACRLLRIGWESAQRIMERGVQRGLERRSVAGIK
                     HAGMDEKSFKRGQSYITTLNDIDADNARVLEVVEGRTHQAAVTLLESIPAAQRSEVRA
                     VALDMWDPFLKAVRQVLPEADIVHDKYHVTAHLNTAVDTVRKAEHTRLLAAGDDTLKG
                     SKYQWLRTYCDGRSSEAVSFRKLHQLDLKTSRAWHYKEDFRHFWNYRLPDAALRFYEA
                     WRKAVMRSRLEPLKKVVRMIDEHWREIFNYIRHRITNAVSEGLNSRIQAIKSAARGFR
                     SYANYRVHILFHCGMLDLKPSIH"
     gene            3599..3940
                     /locus_tag="PLP58_19850"
     CDS             3599..3940
                     /locus_tag="PLP58_19850"
                     /inference="COORDINATES: protein motif:HMM:NF037202.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ10845.1"
                     /translation="MAEPELKFAKKRDPETNKSINDKTTVIYNSRITLRGIPLEAYDY
                     VVNGKPALEWVMERQSVTTDKDSGIVNDANLWATETMNNPAYPLELFLRVITVSLETL
                     KIVNGLPGMEI"
     gene            4008..4511
                     /locus_tag="PLP58_19855"
     CDS             4008..4511
                     /locus_tag="PLP58_19855"
                     /inference="COORDINATES: protein motif:HMM:NF016060.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HRJ10846.1"
                     /translation="MVKEITATARFIKHNDWATIRRAFLRKDAHPYLQFVKYVICGLA
                     AFTTHQVVWLAASAWFFPSIDSAIPQETRALNSTLSNCIALVFSTAVAYLTNIRWVFT
                     PGRHSRTMEIVYFFAVGAISFAGGLAAGPWMIKVFGLNTLVAQLFMAVTSVLINFVCR
                     KFFIFKS"
     gene            4625..>5862
                     /locus_tag="PLP58_19860"
     CDS             4625..>5862
                     /locus_tag="PLP58_19860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009961385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arylsulfatase"
                     /protein_id="HRJ10847.1"
                     /translation="MKRLLTALILLAAPHACLSAGLAGSRPNILFILTDDQGYGDMSA
                     HGNPVLKTPNLDRLRAEGRHFSDFNVSPTCAPTRSALLTGRHEFRNGITHTILERERM
                     DPKAVTIAQVLKSAGYTTGIFGKWHLGDEKEYRPRARGFDEQFIHGGGGIGQSYPGSC
                     GDAPGNLYFDPAILHNDSFVRTKGYCTDVFFEQAARWIEGVKGKQPFLCWLATNAPHA
                     PYIARPEDRALYEGRGLGENVENFFGMIHNIDENIGRLRARLEQWGIANDTLIVFIND
                     NGTAAGHSVFNAGMRGQKGSPWLGGIRAISLWNWPGRIQPGECGALTAHVDVFRTWAA
                     LAGASLPEAAQQQAEGRDLLPLLENPAAPWDERVLFTHVGRWPKGQDYHAFKDRNASV
                     RDSRWQLVSAAQPARKGQPAP"
BASE COUNT         1195 a         1886 c         1789 g          992 t
ORIGIN      
        1 cccccaaaga ccgctcggag gagttttgac acagtctcgc cagaatgcgg ggcctgggtg
       61 tcgcggcgga agcacatccc gcgctttggc aagcgcgcct acagagcact gatgccacat
      121 gcaaacgcca gtaattcaag gctcgacatg ccatgacccc ccgggataaa agcgggtcat
      181 gaaaaccacc atgccgcgtc ccattgaggg catcgccgcc cacgtcccac gcatggcctt
      241 gctgcttctg gcctgttccg tcagcgcatt gcaggcgcag gagatggtga cgcgctcctt
      301 cgatgtgccg acgaagttcc cccggctggt gggggagatt ttcccaaagc ctgaaagcgg
      361 cgaatcacaa ccctacgacc ccttcagccc cgacaatcct ccaggcttgc ggccgcagga
      421 gttcagcaaa agcacgctgc aaatcctgag ggacgccggg ctgcccttcc cggaaggctc
      481 cgccgccttt cacgatgcca cgcatcatgt gctgaccgtg cgcaacactc cggcaaacat
      541 gaagatcgtg gagcaggtca tcgccacgct gaatgaggag ttgccggtga cattgtccat
      601 ccgggcggac ctagtcgagg cgcctggcag tgaactcagg cggatcgccg cgctggactt
      661 tgagaaagcc gcgcccgcct tggcggccct gctcaagcag gcgttcgtgc ccggatcgaa
      721 cgtgcgcatg gtcaggacgg ccctggtcga atcccagagc ggcatgcgaa gtgacatcct
      781 ttcgggattc gaggactggg agcacagcgg gatcgaggcc ggggaaaacg gccatgttat
      841 ccagatcgag aagacagccc gaggattcca tttcgaaacc gatgcgtatg gcagcaactc
      901 cgcccttcat ggacgcggca ttgatttgaa ctttgcagtc agtcatcacc cgctgctgaa
      961 ccaaatcaac gaagtcgagc cgcaggagcg catgccattc cagcatggca gagattccat
     1021 atccacctcc atccaacccc gtgacgggct gccctgcctc gccggacttt gggacgctcc
     1081 cagccagaac caggacgtgc tccaagccgt ggtgatcacg gtcaagacca tcattcacaa
     1141 acccctgcgc acgaaatgga acaatgcgtt caggccaaat ctggaggaac ccatcgccaa
     1201 gtcagttcag catgtctttg acctgccaaa ggacgcatgg actctgcccg cgcgcttcgg
     1261 cctgggggag aacaaggaga ttgcggacga tctgcggcag gcagacatcc aattgccgga
     1321 agacgcgcgg gtcagcctgg agactcccca caaactctcc gtgcaggcgg acatggaaac
     1381 gatggaggtc atccagcgct ggctggtcga gcagcatcgc gggcaagcga caagcctgga
     1441 catgaccctg gagatcgtgc gcggtccggc aagcctgctg cgtcccatgc tggaggacat
     1501 cggggattcg ggcgatgaca catcgctatg gagtcaggcg catgaggcgg tgcggagggg
     1561 cgaggcgcgg atcatcagca cgctgcacgc gcgggttgat tcgaagcatg aagtccagct
     1621 cactgcgggc aatggcgaga ggcgtttgaa cgaagtcggt tggactcagg ggcgggatca
     1681 gcatgcctgg ctcgctaccg aggagggatt caccggcgtg aaactgaagc tcttacccac
     1741 gcttggtgaa gacggccaga ccatggcggc ggagtgcaag ctggagtgga accccgcccc
     1801 ggccattccg gggcggatgg ccatccctga ggcatcggat ggcggaatcg agctgccgca
     1861 catccgcgag cggcgcatgg agctttcctc ccacatggtg ctcatggatg gcgccaccaa
     1921 actcatcggt gcatggaatc cggagagcga ggaagccggg gatgcgggaa gcattatgga
     1981 actggccttc atgaaggttc attttgcacg gcatccggga ggtgccccgg tggatgcaac
     2041 cacccgcccg ctgcctccag gcgtcgagcc gcctccgcca cccaagcccg gaatggaaac
     2101 acgagtgttc cgtgtgtctc ccagttttct aagctctgcc aaattacctt ccgatgaaag
     2161 cgatccgtcc ttgcctgtct catctgccaa aaagatattt tctgaccacg gctttcccct
     2221 cccagaggga agcacaaatt gattcttcgc gacttttaat gggtgagagg gagctgtaaa
     2281 gtgagcggat gagcacattg ccgagcctca atgaccacta cagccttctt ttgggactcg
     2341 acaagcactg ggaggtggag cgggtggatt tgcaactggc agccaaaagg gtgcgcattg
     2401 acctgcggca tgtccgtggt gcggcgctgt cctgtccgca gtgtcagaac gcctgcgtca
     2461 tgttcgacca cgccccggag cgtgagtggc ggcacctgga caccatgcag tttgaaacgg
     2521 ttctgcgagc acgggtgcca cgctgcaagt gcgaggactg cggcgtcaaa accattgccg
     2581 tgccttgggc gggaaagcat tcacccttca cctggttgtt tgaagccttc gccatcgcgg
     2641 tgctgcagag tgccggcagc accagtgatg cctgccgact gctgcgcatc ggctgggaga
     2701 gtgcgcaacg catcatggaa cgtggcgtgc agcgtggcct ggagcggcgc agcgtggcag
     2761 ggataaagca tgcgggcatg gatgagaaga gcttcaaacg cggacagagc tacatcacca
     2821 cgctcaacga cattgatgcg gacaacgcac gcgtgctcga agtggtggag gggcgcaccc
     2881 atcaagccgc cgtgaccttg ctggagagca tccccgcggc gcagcgatcc gaagtgcggg
     2941 ccgtcgcact ggacatgtgg gatccttttc tcaaagccgt gcgtcaagtg ctgccagagg
     3001 ccgacatcgt gcatgacaag tatcacgtca ccgcccatct caacactgcg gtggacaccg
     3061 tgcgcaaggc cgagcacacg cgactgctgg ctgccggtga tgacacgctc aagggcagca
     3121 aatatcaatg gctgcgcact tactgcgacg ggcgcagcag cgaggcggtg agttttcgca
     3181 agctgcacca gctcgatctg aaaaccagcc gcgcgtggca ctacaaggag gactttcggc
     3241 acttctggaa ctaccgttta ccagatgccg cactgcgctt ctacgaggcc tggcgcaagg
     3301 cggtgatgcg cagccgcctg gagccgttaa aaaaggtcgt gcgcatgatc gacgagcatt
     3361 ggcgcgagat cttcaactac atcaggcacc gcatcaccaa cgccgtgagt gaaggactca
     3421 acagccgcat ccaagccatc aagagcgccg ccaggggctt tcgctcctat gccaactacc
     3481 gggtgcacat cctcttccat tgcggaatgc ttgacctgaa accatccatc cattaaatcc
     3541 cgcgtagaac ctatctagaa aacggccagt ccgcgccacc aatgaagctc aggcacttat
     3601 ggctgagcca gaactgaaat tcgccaagaa gcgcgacccc gaaacgaaca agagcatcaa
     3661 cgacaagacc accgtcattt acaactcccg gatcacgctg cgcggcatcc cgctggaggc
     3721 ttatgattat gtggtgaacg gcaaacccgc cctcgaatgg gtcatggagc gccagtccgt
     3781 gaccaccgac aaagacagcg gcatcgtcaa cgacgccaac ctctgggcca ccgaaacgat
     3841 gaacaacccc gcctaccccc tggaactctt cctccgcgtg atcaccgtga gcctggagac
     3901 gctgaagatc gtgaacgggc tgccgggaat ggagatttga gcggggtggt gaggcacttg
     3961 tcaccgcccg ccgcttgcat tcgcgcgggg cgcgggtaaa gccgggcatg gtcaaggaaa
     4021 tcaccgccac ggcgcggttc atcaagcaca acgactgggc gacgatccgg cgcgccttcc
     4081 tccgcaagga cgcgcatcct taccttcagt tcgtgaagta tgtgatctgc gggctggcgg
     4141 ccttcaccac gcaccaggtg gtgtggctgg ctgcgtcggc gtggtttttc ccctcgattg
     4201 actcggccat cccgcaggag acgcgcgcgc tcaacagcac tttgagcaac tgcatcgccc
     4261 tggtcttcag cacggcggtg gcttacctga cgaacatccg ctgggtgttc acgcccggac
     4321 gccacagccg gacgatggag atcgtgtact tcttcgcggt gggcgccatc agcttcgcgg
     4381 gcgggctggc ggcggggccg tggatgatca aggtcttcgg cctgaacacg ctggtggcgc
     4441 agctcttcat ggcggtgacc tcggtgctga tcaatttcgt ctgccggaag ttcttcatct
     4501 tcaaaagctg agcggcctcc gggcggggcg gcccggcgct tgcgggcggg gcgggcgcgg
     4561 aggttgtgat ccgggcatga gtttgcataa gttccggcgc gcgcgcgccg ttactggcgg
     4621 ctccatgaaa cgcctgctca ccgccctcat cctcctcgcc gcgccccatg cctgcctcag
     4681 cgccggactc gccggctcgc gcccgaacat ccttttcatc ctcaccgacg accagggcta
     4741 cggcgacatg tccgcccacg gcaaccccgt cctcaagacg ccgaacctgg accgcctgcg
     4801 cgccgaaggc aggcacttct ccgacttcaa tgtcagcccc acctgcgcgc ccacccgcag
     4861 cgcgctgctg accgggcggc acgagttccg caacggcatc acccacacca tcctggagcg
     4921 cgagcgcatg gacccgaagg ccgtgaccat cgcccaggtg ctcaagtccg ccggctacac
     4981 caccggcatc tttggcaaat ggcacctcgg tgatgaaaag gaataccgcc cccgcgcgcg
     5041 cggcttcgac gagcagttca tccacggcgg cggcggcatc ggccagagct accccggcag
     5101 ttgcggagac gcgccgggca atctctactt cgaccccgcc atcctccaca atgacagctt
     5161 tgtgaggacg aagggttact gcacggatgt cttcttcgag caggcggcca ggtggatcga
     5221 gggcgtcaag ggcaagcagc cctttctctg ctggctggcc accaacgccc cgcacgcgcc
     5281 ctacatcgcc cgtccggagg accgcgcgct gtatgagggc aggggactcg gcgagaatgt
     5341 cgagaacttc ttcggcatga tccacaacat agacgaaaac atcggcaggc tgcgcgcgcg
     5401 gctggagcag tggggcatcg ccaacgacac gctgatcgtt ttcatcaatg acaacggcac
     5461 cgccgccggc cacagcgtct tcaacgccgg catgaggggg caaaagggca gcccatggct
     5521 cggcggcatc cgcgccatct ccctgtggaa ctggcccggc cgcatccagc ccggtgaatg
     5581 cggcgcgctc accgcgcatg tggatgtctt ccgcacctgg gcagccctgg ccggcgcgtc
     5641 cctgccggag gccgcgcagc agcaggccga ggggcgcgat ctcctccccc tcctggaaaa
     5701 ccccgccgcc ccctgggacg agcgcgtcct cttcacccac gtcggccgct ggcccaaggg
     5761 ccaggactac cacgccttca aggaccgcaa cgccagcgtc cgcgacagcc gctggcagct
     5821 cgtcagcgcc gcccagcccg cgcgcaaagg ccagcccgcc cc
//
LOCUS       DAOMYJ010000276         5816 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Prosthecobacter sp. isolate AMR_MDS_5629
            NODE_4697_length_5816_cov_21.791876, whole genome shotgun sequence.
ACCESSION   DAOMYJ010000276 DAOMYJ010000000
VERSION     DAOMYJ010000276.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867850
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prosthecobacter sp. (wastewater metagenome)
  ORGANISM  Prosthecobacter sp.
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Verrucomicrobiaceae; Prosthecobacter.
REFERENCE   1  (bases 1 to 5816)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5816)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.30
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:34:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,742
            CDSs (total)                      :: 4,684
            Genes (coding)                    :: 4,676
            CDSs (with protein)               :: 4,676
            Genes (RNA)                       :: 58
            tRNAs                             :: 55
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5816
                     /organism="Prosthecobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4697_length_5816_cov_21.791876"
                     /isolate="AMR_MDS_5629"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1965333"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..705)
                     /locus_tag="PLP58_19865"
     CDS             complement(<1..705)
                     /locus_tag="PLP58_19865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ10848.1"
                     /translation="MLGLGGGDFPFRPEWQRFDNTRVLWSSYSKDSGRTWSEPRFLAA
                     LVDASEGAGIRKITLRPERGAVLALSFENGRGKRFEMRFKEYELRKLATVDDLFNAPA
                     SRFVLRSTVAGSDDTTLADAVCVGRDIRLTPDHLPEECNLRRDLGLIVAQSVEGQLVH
                     TARSQSKTLFETRAVVTPKGDYLVFIPDGSHGNTLRKNGNLLTAYRSSDQGRTWQRPF
                     YPFGEGTHYGVLPMVPA"
     gene            complement(724..2235)
                     /locus_tag="PLP58_19870"
     CDS             complement(724..2235)
                     /locus_tag="PLP58_19870"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialidase family protein"
                     /protein_id="HRJ10849.1"
                     /translation="MDVVVVNGIEISAKSPPKGLVLDKHTGLLTVEAVQGRVVHRTQT
                     RILETRATITPRGDYLLMFPEGDHYAKSKGEKINSMMACRSTDKGKTWSKPAVAYDIP
                     YGQHGFIPLTPRGTQTIHCFGTQPVPGKWTWEDGKRENAPIGWRTSEDDGHTWSDVRL
                     IEPVNDPGFMGMSVMRMTETDAGTWLLGSHLADWSVKPFTTRQYLLRSEDKGKTWSVL
                     PGARPKGWQAAGFNRMDEGRPLNLGGGKVLFMSRTPQGHLFTAWSQDDGKTWTDPAPS
                     TLVHPDAPPMLFPLSDGKTLVAFHHNKVPVTNHGDLNDHSELMRMRSEIWASLSTDEG
                     RTWSEPRFMLANAVAPVHKVSGFNSQCSYLDAFTEDGVMHLFMPHRWQQVLHLTIKES
                     ALKTLPTKAQLLAKQAGSESASVPLMETFPLAGRELNPAAPPLGLKTYSDLGLMIEES
                     ILGYELTAGQPAPPPAAAAASDTDTTGRMDPGAADLSVLTPLETTGGSLVFPL"
     gene            2551..>5816
                     /locus_tag="PLP58_19875"
     CDS             2551..>5816
                     /locus_tag="PLP58_19875"
                     /inference="COORDINATES: protein motif:HMM:NF024753.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HRJ10850.1"
                     /translation="MKLTRFAFGALLLPSLLPAADWPQWRFDAGRSAASEERLPEKLT
                     AVWERGYGAREQVWDDPLNHDLMSYDRVFEPVVLGNRMFLGFNDHDKVVALDLDTGAE
                     VWTFFTGGPVRLPPACHDGKVVFASDDGFLYCVSAEEGKLLWSFQGAPSARKALGNRR
                     LISAWPARGGPVIRDGRVYFAASIWPFMGTFLYALDVETGKVVWVNDGTGADFIKQPH
                     SASSFAGVAPQGALVAVKDTLLVPGGRSVPAAFDLATGGLKYFHLNDGGKGNGGAFVI
                     ANDEFYFVHTRERGVRVYDLKTGTAGKSEMNEPVLHGGRFYTAAESKGKRPVVRALGT
                     SEKVYWEIEADATGDLIKAGGRLYAAGKDKLTAIELPEKKDGKPRVAWSLPVKGEVRR
                     LLAANGRLVAVTLDGRILVHGAEKGPGVIAERMDPAPEVPAAARETARRLLAGVEDKG
                     GHALIFGSDESGLVDALLAESEMQLTVVEPDAARVAKLRGEYDARGLYGKRVTFHEGD
                     AVSFQAPPYIAPLVVVGGSQAERVGDEAFMQAAYASVRPYGGVLHVAGAQVNLKPGML
                     ESGEAKVHETGTLVVRAGALPGAADWTHQYGDIGNTVKSDDSRVRAPLGVLWFGGSSN
                     LDVLPRHGHGPPEQVVGGRLFIEGMNSLSARDVYTGRVLWKHDFGDLGTFGIYYNETY
                     FDDPLNTAYNQKHIPGANGRGTNFVATEDSVYMALKGECQVLDARTGDLRRTVKLRED
                     KKDPAQWAYIGVYEDLLIGGDDYAHFSLKLGGAKSRAESSIEDMSASDGLAVFDRHTG
                     KALWRVKARHSFIHNGIVAGNGRIYCLDKLTKHAEDLLKRRGQDLPGDYRIVAFDART
                     GEEVWQHEKDIFGTWLGYSAQHDVLLQAGAKASDRLSSEVGTGMAAYQGADGKVRWSD
                     LKREHTGPCILHNELILTGANSYSSSGGAFNLLTGEPHLVLNPLTGKMEPWRLNRTYG
                     CNTIIASEHLLTYRSGAAGFYDLESMSGTGNLGGFKSGCTSNLVVANGVLNAPDYTRT
                     CSCAYQNQTSLALIHMPDMEMWTYSQFGLDGDQGDRVLRAGINFGAPGNRRASDGTLW
                     L"
BASE COUNT         1024 a         1680 c         2047 g         1065 t
ORIGIN      
        1 agcgggcacc atgggcagca cgccgtagtg ggtgccctcg ccgaaggggt aaaaggggcg
       61 ctgccaggtg cggccctgat cgctggagcg ataggcggtc aggaggttgc cgtttttgcg
      121 gagggtgttg ccgtggctgc catcgggtat gaaaacgaga tagtcgccct tgggagtcac
      181 cacggcgcgc gtttcgaaaa gcgttttgct ttgcgaacgc gcggtgtgga caagctggcc
      241 ttcgacggat tgcgccacaa tcaggcccag atccctgcgg aggttgcatt cctccggcag
      301 gtggtccggc gtgagacgga tgtcccggcc cacgcagacc gcgtcggcca gcgtggtgtc
      361 atcgctgcca gccacggtgc tgcgcaggac gaagcggctc gctggcgcat tgaagaggtc
      421 atcaacggtt gccagtttgc gcaactcata ctccttgaac cgcatttcga atcttttgcc
      481 ccggccgttt tcgaatgaca aggccaggac agcgccgcgt tcaggacgca gcgtgatctt
      541 gcggatgccc gcgccctcgg aagcgtccac caaggcggcg agaaagcgcg gctcgctcca
      601 ggtgcgcccg ctgtccttgg agtaggaaga ccagagcacg cgggtgttgt cgaagcgctg
      661 ccattccggg cggaagggaa agtccccgcc gccaagaccg agcatgatct gacagttttt
      721 gacctagagc ggaaaaacga gcgagccacc cgtggtctcg agcggtgtca ggactgacaa
      781 gtctgcggcg ccaggatcca ttcgaccggt ggtatcggtg tcgctggcag cagctgcggc
      841 tggcggcgga gctggctggc cggctgtgag ttcgtatcca aggatggatt cctcgatcat
      901 cagccccagg tccgagtaag tcttgagacc cagaggaggc gccgcagggt tcaactcccg
      961 gcccgcaagg gggaaagttt ccatcaaagg cacgctcgcc gactctgaac cggcctgctt
     1021 tgccaggagt tgcgctttcg ttggcagcgt cttgagcgcg gattccttga tggtgagatg
     1081 cagcacctgc tgccagcggt gcggcatgaa gaggtgcatg acgccgtcct cggtgaaggc
     1141 gtcgaggtag gaacattgcg agttgaagcc gctgaccttg tggacgggcg cgacggcgtt
     1201 ggcgagcatg aagcgcggtt cgctccaggt gcggccttcg tcggtggaga gcgatgccca
     1261 gatttcgctg cgcatgcgca tcagttccga gtggtcgttg agatccccgt ggttcgtgac
     1321 agggaccttg ttgtgatgaa aggcgacgag cgtcttgcca tcgctgagcg ggaacagcat
     1381 cggcggcgcg tcgggatgca ccagcgtgct cggcgcgggg tcggtccagg ttttgccgtc
     1441 gtcctggctc catgcggtga acagatgccc ctgcggcgtg cgtgacatga agagcacttt
     1501 gccaccgccg agattcagcg gccggccctc gtccatgcga ttgaaacctg cggcctgcca
     1561 gcctttcggc cttgctccag gcagcacgct ccatgtcttg cccttgtcct cgctgcgcaa
     1621 aagatactgc cgcgtggtga acggcttcac cgaccagtcg gcgagatgcg agccaagcaa
     1681 ccaggtgccc gcgtctgtct ccgtcatgcg catgacggac atgcccatga agccgggatc
     1741 gttcaccggc tcgatgagac gcacatcaga ccaggtgtga ccgtcgtctt cggaggtgcg
     1801 ccagccgatg ggagcgtttt cgcgtttgcc gtcctcccag gtccatttgc cgggcaccgg
     1861 ttgcgtgccg aaacaatgga tggtttgcgt gccgcgcggg gtcagcggga tgaagccgtg
     1921 ctggccatag gggatgtcgt aggccacggc gggcttgctc catgttttgc ctttgtcggt
     1981 ggatcggcat gccatcatgg agttgatctt ctcgcccttg ctcttcgcgt agtgatcgcc
     2041 ctcgggaaac atgagcagat agtcgcccct cggggtgatg gtggcacggg tttcaaggat
     2101 gcgcgtctgg gtgcgatgca cgacccggcc ttggacagcc tcgacggtga gcaagcccgt
     2161 gtgcttgtcg agcacgagac ccttgggcgg tgatttggcg ctgatctcga tgccattcac
     2221 gacgacgaca tccatgagtg gcaaaggttc aagcgcgtga gccgctgcaa tggcaagcag
     2281 tggggcaaag aaaatctgat tcatgtcttc aagacgcgcc gcgaagtcgc atgttgcttg
     2341 gtcaatacag atgacctgtc tgtatgatgc cgctggtttt gaggggtctt tcagctcgga
     2401 ggctgaagca tgggagatgt gtccagatgt cgggaggcat gccacctgga agggttcagg
     2461 tcgttgcatg gcatggacga tatgtgccca actcagaaaa aactcctttc gcaaaggagc
     2521 cgctgctggc ggcgttgtgc tttgcccatc atgaagctga cccgattcgc ctttggcgcg
     2581 ctgctattgc catcccttct ccccgcagcc gactggccgc agtggcgctt tgatgccggg
     2641 cgctcggcgg cttcggagga gcggctgccg gaaaagctga cggctgtctg ggagcgcggc
     2701 tacggcgcgc gggagcaggt gtgggatgat ccactgaacc atgacctgat gtcttatgac
     2761 cgcgtctttg aaccggtggt gctgggcaac aggatgttcc tcggcttcaa tgaccacgac
     2821 aaggtggtgg cgctggatct ggacacgggc gcggaggtgt ggacgttttt cacgggcggt
     2881 ccggtgcgcc tgccgccggc ctgtcatgac ggcaaagtgg tctttgccag tgacgacggc
     2941 tttttgtact gcgtgtcggc ggaggagggg aagctgctgt ggtccttcca gggcgcgccc
     3001 tcggcgcgca aggcgctggg caaccgccgt ctgatctcgg cctggccggc gcgcggcggg
     3061 ccggtgatcc gggatggcag ggtctatttt gcggcgagca tctggccgtt catgggcacg
     3121 ttcctttatg cgctggatgt ggagacgggg aaggtggtgt gggtgaatga cggcacgggg
     3181 gcggacttca tcaagcagcc gcacagcgcg tcgtccttcg caggtgtggc gccgcagggg
     3241 gcgctggtgg cggtgaagga cacgctgctg gtgccgggag ggcgctcggt gccggcggcc
     3301 tttgatctgg cgactggcgg gctgaagtac ttccacctca atgacggcgg caagggcaat
     3361 ggcggggcgt ttgtgatcgc gaacgacgag ttttatttcg tgcatacccg agagcgcggg
     3421 gtgcgggtct atgatttgaa aacgggcacg gcgggcaagt cggagatgaa cgagccggtg
     3481 ctgcacggcg gacgctttta cacggcggct gagtccaagg gtaagaggcc tgtggtgcgc
     3541 gcgctgggca cgagcgagaa ggtgtattgg gagatcgagg cggatgcgac gggggatttg
     3601 atcaaggcgg gcggccgcct ttatgccgca ggcaaggata agctgacggc catcgagttg
     3661 ccggagaaaa aggatggcaa accgcgagtg gcgtggtcgc tgccggtgaa gggggaggtg
     3721 cggcggctgc ttgcggcgaa tggcaggctc gtcgcggtga cgctggacgg gcgcattctg
     3781 gtgcatggcg cggagaaggg gccgggcgtg atcgcggaga ggatggatcc cgcgcctgag
     3841 gtgcccgccg ccgcgcgtga gacggcgcgg cgcctgctgg cgggtgtgga ggacaagggg
     3901 ggacacgctt tgatcttcgg ctcggatgaa agcggcctgg tggacgcgct gctggcggag
     3961 tcggagatgc agctcacggt ggtggagccg gacgcggcgc gggtggcaaa gctgcgcggc
     4021 gagtatgacg cgcgcgggct ttatggaaag cgtgtgacgt ttcatgaggg cgatgccgtt
     4081 tccttccaag cgccgccata catcgcaccg ctggtggtgg tcggcggttc gcaggcggag
     4141 cgggtggggg acgaggcgtt catgcaggcc gcgtatgcct cggtgcggcc ctatggcggg
     4201 gtgctgcatg tggcgggcgc gcaggtgaac ttgaaacctg ggatgctgga gagcggtgaa
     4261 gccaaagttc acgagaccgg cacccttgtg gtacgcgccg gcgcgctgcc cggcgcggcg
     4321 gactggacgc atcaatacgg cgacatcggc aacacggtga agtccgacga cagccgcgtg
     4381 cgcgcgccgc tgggcgtgct gtggttcggc ggcagctcga acctggacgt gctgccgcgc
     4441 cacggccacg gcccgccgga gcaggtggtg ggcgggcggc ttttcatcga gggcatgaac
     4501 agcctcagcg cgcgcgatgt gtacacgggc cgcgtgcttt ggaagcatga cttcggcgac
     4561 ctgggaacct ttggcattta ttacaacgag acctacttcg acgatccgct caacacagcc
     4621 tacaaccaaa agcacatccc cggcgccaac gggcgcggca cgaacttcgt cgccaccgaa
     4681 gactcggttt acatggcctt gaaaggcgag tgccaggtgc tggacgcgcg cacgggcgat
     4741 ctgcggcgca cggtgaaact gcgcgaggac aaaaaagacc ccgcgcagtg ggcctacatc
     4801 ggcgtgtatg aggacctgct catcggcggc gatgactacg cgcatttcag cctcaaactg
     4861 ggcggggcaa aaagccgcgc ggagtcgagc atcgaggaca tgtcagccag cgacggcctc
     4921 gccgtttttg accgtcacac cggcaaagcg ctctggcgcg tgaaagcgcg gcacagcttt
     4981 atccacaacg gcatcgtggc cggcaacggc cgcatctact gcctcgacaa gctcaccaag
     5041 cacgcggagg acctgctcaa gcggcgcggg caggatttgc ccggggacta ccgcattgtg
     5101 gccttcgacg cgcgcaccgg ggaggaagtg tggcagcatg agaaggacat ctttggcacc
     5161 tggctcggct actccgcgca gcatgacgtg ctgctgcaag ccggtgccaa ggccagcgac
     5221 cgcctgtcct ccgaggtcgg caccggcatg gcggcgtatc agggcgcgga tggcaaagtg
     5281 cgctggagtg acttgaagcg cgagcacacc ggcccctgca tcctgcacaa tgagttgatc
     5341 ctcaccggcg ccaactccta ctccagcagc ggcggcgcat tcaacctgct caccggcgag
     5401 ccgcatctcg tgctcaaccc gctcaccggc aaaatggagc cctggcggct gaaccgcact
     5461 tatggctgca acaccatcat cgccagcgag catctgctca cctaccgcag cggcgcggcc
     5521 ggcttttatg atctggaaag catgagcggc accggcaacc tgggcggctt caaaagcggc
     5581 tgcacctcga acctcgtcgt ggccaacggc gtgctcaacg cgcccgacta cacccgcacc
     5641 tgctcctgcg cgtatcaaaa ccagacctcg ctggccctca tccacatgcc ggacatggaa
     5701 atgtggacct acagccagtt cggcctcgac ggtgaccagg gtgaccgcgt gctgcgcgcg
     5761 ggcatcaact tcggcgcgcc cggcaaccgc cgcgccagcg acggcacgct ctggct
//
LOCUS       DAOMYJ010000277         5795 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Prosthecobacter sp. isolate AMR_MDS_5629
            NODE_4720_length_5795_cov_20.639199, whole genome shotgun sequence.
ACCESSION   DAOMYJ010000277 DAOMYJ010000000
VERSION     DAOMYJ010000277.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867850
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prosthecobacter sp. (wastewater metagenome)
  ORGANISM  Prosthecobacter sp.
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Verrucomicrobiaceae; Prosthecobacter.
REFERENCE   1  (bases 1 to 5795)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5795)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.30
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:34:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,742
            CDSs (total)                      :: 4,684
            Genes (coding)                    :: 4,676
            CDSs (with protein)               :: 4,676
            Genes (RNA)                       :: 58
            tRNAs                             :: 55
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5795
                     /organism="Prosthecobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4720_length_5795_cov_20.639199"
                     /isolate="AMR_MDS_5629"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1965333"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..449)
                     /locus_tag="PLP58_19880"
     CDS             complement(<1..449)
                     /locus_tag="PLP58_19880"
                     /inference="COORDINATES: protein motif:HMM:NF019262.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ10851.1"
                     /translation="MLLLPSLLHAADYEREIKPLLHERCFACHGALKQKAGLRLDTVA
                     LMRQGGDEGDILAGGAPLILQRVTTHDEDDLMPPEGEGARLSEEQVVLLKEWIAAGAP
                     GPANEQAEPDPNAHWAYHLPQDHGQGVDALLEKKLAARGLRAQPPAAP"
     gene            584..1438
                     /locus_tag="PLP58_19885"
     CDS             584..1438
                     /locus_tag="PLP58_19885"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HRJ10852.1"
                     /translation="MHLVTLAWGLTAILGKLITLGPVDLVVWRTLLAALGFGLLAVVA
                     GRRLALPRAEALRLLGTGALLGLHWVLFFASARLATASVCLAAMPTGILWCSLIEPWV
                     DGTRRWRFSELGVGAVIIGAVWMIYKVEFHHVGGFTVGLAAAALAALFVIANKHWAGR
                     AHHLARGAWQMSGALLVTLGAAFLYAGAPPALPAPQDWLWLALLSGVCTVGAYAGYMV
                     ALGHLSVFTTNVIYNMEPVYGIVLAMLVLGAEERMSGGFYAGAAIILACVISVPWLKG
                     RAERVEMA"
     gene            1435..2367
                     /locus_tag="PLP58_19890"
     CDS             1435..2367
                     /locus_tag="PLP58_19890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007413123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HRJ10853.1"
                     /translation="MSHTTRRVFLAASLSAAASRIAAQEDAGAEPVTDIHQHTNYRGR
                     ADEHLLAHQKAMGVTTTILLPAGTPIDTPSTNQGKSNGLAAGCGTTDTCMALARAHPG
                     ACLWGANEVTDHENAAAEIEKWLKQGACIIAEQKFHVECDSAQSQRLYALAADYGVPI
                     LLHFQEGAYNLGYERFHAMLAKFPKTSFIGHAQTFWGHIDKGHDPKALYPKGPVTPGG
                     LTDRYLADYPNMFADMSAGSGLNALTRDEDHTRGFLERHQDKLLYGSDCTDHLGRGPA
                     CQGAQTLAMIRKLAATRAIERKILHGNAARLFRL"
     gene            complement(2371..3603)
                     /locus_tag="PLP58_19895"
     CDS             complement(2371..3603)
                     /locus_tag="PLP58_19895"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="vanadium-dependent haloperoxidase"
                     /protein_id="HRJ10854.1"
                     /translation="MLSPEIARDMAMLQTAIYNAVEGIAGDHEIFASGAYLGPSGTAP
                     AGASMEAATAAAAFTILQSLHPGFVGDFATLYTTQLSGIAEGAAKTDGINFGTLVAND
                     ILNWRALDGAADASDPGLYTPVGTIGHWQPTPPGTGALPGWGNVTTFGISGTVGFTTS
                     LPGPTREDYLATGQYAADYQQVLDLGGLTSLARTADQLDAAFFWSGADGTVTVAGMWN
                     QIAQTVAASEGMSLQDTARLFAALNVALADAAIVAWETKYNVDFWDPLSAIVNGEFDG
                     NGATLGDEFWSSLLGLDSLAPSYFSDHSALSAAAAAVLASFFGDAHAFSLDSDTDGNG
                     TFDMTRLFSSFSGAAEEAGMSQIWGGMSYETGHLDALDSGSAVGNDVMGSYFAPVPEP
                     SGLALVILGAVLLSRRRA"
     gene            complement(3635..3793)
                     /locus_tag="PLP58_19900"
     CDS             complement(3635..3793)
                     /locus_tag="PLP58_19900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ10855.1"
                     /translation="MLEYRPSEGIVQAFHGFIDVQFSSFPGHPAAFLPSAPEGGSGAG
                     VIVGAGLE"
     regulatory      3899..4010
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            4075..>5795
                     /gene="thiC"
                     /locus_tag="PLP58_19905"
     CDS             4075..>5795
                     /gene="thiC"
                     /locus_tag="PLP58_19905"
                     /EC_number="4.1.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007415738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomethylpyrimidine synthase ThiC"
                     /protein_id="HRJ10856.1"
                     /translation="MIADPTTSFEPHSSEQLPASTRVYVEGQIHKDVRVPMREIALSP
                     TKSFNGRIEVNEPVRVYDTSGPWGDPSYKGTVEEGLPALRKAWILSRNDVEEYTGRAI
                     EPRDNGYLTANHAEYAAAKREGLLSPLKAPINAQRNPLRSTGKPVTQLHYARQGIITP
                     EMEFIAIRENMRRAQIADFKDDILRNDLDKQHAGSATLQHSTTPSIFRRFPQRIPAEI
                     TPEFVRSEVAAGRAIIPANINHPELEPMIIGRNFLVKINANIGNSAVASSIEEEVEKM
                     RWATKWGADTVMDLSTGKNIHATREWILRNSPVPIGTVPIYQALEKVNGKAEDLTWEL
                     FRDTLIEQAEQGVDYFTIHAGVLLRFVPLTASRMTGIVSRGGSIMAKWCLAHHKENFL
                     YTHWDDICDIMAAYDVSFSIGDGLRPGSIADANDKAQFGELEVQGELTQRAWAKGVQV
                     MNEGPGHVPMHMIEENMAKQLEWCHEAPFYTLGPLTTDIAPGYDHITSGIGAAMIGWY
                     GCAMLCYVTPKEHLGLPNKEDVKAGVITYKLAAHAADLAKGHPGAQYRDNALSKARFE
                     FRWEDQF"
BASE COUNT         1071 a         2131 c         1698 g          895 t
ORIGIN      
        1 ggcgcggcgg gcggctgggc gcggaggccg cgcgcggcga gctttttctc cagcagcgcg
       61 tccacgccct ggccgtggtc ctggggcaaa tggtaggccc agtgcgcgtt cgggtccggc
      121 tccgcctgct cgttcgctgg accgggagcg ccggcggcga tccattcctt gagcaagacc
      181 acctgctcct cgctgaggcg cgcgccctcg ccctcgggcg gcatgaggtc gtcctcgtca
      241 tgggtggtga cgcgctggag gatgagcggc gcaccgcccg caaggatgtc cccctcgtcc
      301 ccgccctggc gcatgagggc cacggtgtcg aggcgcaggc cggccttttg cttgagcgcg
      361 ccgtggcagg cgaagcagcg ctcatgcagc agcggcttga tctcgcgctc ataatccgcc
      421 gcgtgcagca gggacggcag aaggagcatg aaaaaaacgc ggcgcatggt ctggacggac
      481 ttggaaggta cgtctttgcg catcccgcgc tttcgcgcgc tgcggttgcc aaatcagcgc
      541 cccgcattat gggaatgcgc caatgacgcg ccatcatctt ctcatgcacc tggtcacgct
      601 ggcctggggc ctgacggcga tcctgggcaa gctcatcaca ctcggcccgg tggacttggt
      661 ggtgtggcgc actctgctgg ccgcgctcgg attcggcctg ctggcggtgg tggcaggccg
      721 caggctggcg cttccgcgcg cggaggcgct gcggctgctg ggcacggggg cgttgctggg
      781 cctgcattgg gttttgtttt tcgcctcggc gcgtctggcc accgcgtcgg tatgcctggc
      841 ggcgatgccg acgggaattc tttggtgttc cctcatcgaa ccctgggtgg acggcacgcg
      901 gcgctggcgc ttttccgaat tgggcgtggg cgcggtcatc atcggggcgg tgtggatgat
      961 ttataaggtg gagtttcacc atgtgggcgg ctttacggtg gggctggctg cggcggcgct
     1021 ggcggcgctg ttcgtcatcg ccaacaagca ctgggcgggc cgcgcgcatc atctggcgcg
     1081 gggtgcgtgg cagatgagcg gggcgctgct agtgacgctg ggcgcggcat ttttatacgc
     1141 gggagcgccg cccgcgctgc ccgcgccgca ggactggttg tggctggcgc tgctctccgg
     1201 cgtctgcacg gtgggcgcct acgcgggcta catggtggcg ctcggccatc tgtcggtctt
     1261 taccaccaat gtcatctaca acatggagcc ggtgtatggc atcgtgctgg cgatgctggt
     1321 cctcggcgcg gaggagcgca tgagcggcgg cttttacgcc ggggcggcca tcatcctggc
     1381 ctgtgtcatt tcagtgccct ggctaaaagg gcgcgccgag cgcgtagaga tggcatgagc
     1441 cacactaccc gccgcgtctt tctcgccgcc agcctctccg ccgccgcctc ccgcatcgcc
     1501 gcgcaggagg acgccggggc ggagcctgtg acagacatcc accagcacac gaactaccgc
     1561 ggccgggcgg acgagcacct cctggcgcac cagaaggcga tgggagtgac caccaccatc
     1621 ctgctgcccg cgggcacgcc tatcgacacg ccctccacaa atcaaggcaa gtccaacggt
     1681 ctcgccgccg gttgcggcac cacggacacc tgcatggctc tggcgcgcgc ccaccccggc
     1741 gcctgcctgt ggggcgccaa cgaggtcacc gaccacgaga acgcggcggc ggagatagag
     1801 aagtggctca aacagggggc gtgcatcatt gccgagcaga aattccatgt ggagtgtgac
     1861 tccgcgcaaa gccagcggct ctacgcactg gccgccgact atggcgtgcc catcctgctg
     1921 catttccagg agggcgccta caacctgggc tacgaacgct ttcacgccat gctcgccaag
     1981 ttcccgaaga cgagcttcat cggccacgcg cagaccttct ggggacacat tgacaagggc
     2041 cacgacccca aggcgctcta ccccaagggc cccgtcacac ccggcggcct cacggaccgc
     2101 tacctcgccg actacccgaa catgttcgcc gacatgtccg ccggctccgg cctcaacgcc
     2161 ctgacgcgcg acgaggacca cacgcgcggc tttctggagc ggcaccagga caagctgctc
     2221 tacggcagcg actgcaccga ccacctcggc cgcggccccg cctgccaggg cgcgcagacc
     2281 ctcgccatga tccgcaagct cgccgccacg cgcgccatcg agcgcaagat cctgcacggc
     2341 aatgccgcgc ggcttttccg cctgtgaggc tcaggcccgg cggcggctca gcagtaccgc
     2401 gccgaggatg accagcgcca gccccgaagg ctccggcacc ggggcgaagt agctgcccat
     2461 cacgtcattt cccacggccg agccggagtc cagcgcgtcc aggtggccgg tttcgtagct
     2521 catgcctccc cagatttggc tcatgccagc ctcctccgcc gcgccgctga aggaggaaaa
     2581 caggcgcgtc atgtcaaagg tgccgttgcc gtcagtgtca ctgtccagcg aaaaggcgtg
     2641 ggcatccccg aagaaggacg ccagcaccgc cgccgccgcc gcgctcagcg cgctgtggtc
     2701 ggaaaaatac gagggagcca gcgagtcgag accgagcagg gacgaccaga actcatcccc
     2761 cagcgtcgcg ccgttgccgt cgaactcgcc gttcacgatc gccgagagcg ggtcccagaa
     2821 gtccacattg tatttcgtct cccaggccac gatcgccgcg tccgccagcg ccacattcag
     2881 cgccgcgaac agccgcgccg tgtcctgcag actcatgccc tcgctcgccg ccaccgtctg
     2941 cgcgatctgg ttccacatgc ctgccaccgt caccgtgcca tccgcccccg accagaagaa
     3001 cgccgcgtcg agctggtccg ccgtgcgcgc caggctcgtc agcccgccaa ggtcgagcac
     3061 ctgctggtaa tccgccgcgt attgccccgt cgccagataa tcctcccgcg tcgggccagg
     3121 caggctcgtc gtgaacccca ctgtgccgga gatgccaaag gtcgtcacat ttccccagcc
     3181 cggcagcgcc cccgtgcccg gcggcgtcgg ctgccagtgg ccgatggtgc ccaccggggt
     3241 gtaaaggccc gggtccgacg cgtccgccgc gccatccagc gcgcgccagt ttagaatgtc
     3301 attggccacc agcgtgccga aattgatccc gtccgtcttg gccgcccctt ccgcgatgcc
     3361 gctgagctgt gtcgtgtaca gggtcgcaaa atcccccaca aagcccggat gcaggctctg
     3421 caaaatcgtg aacgccgccg ccgccgtcgc cgcctccatt gaggcacccg ctggagccgt
     3481 gccggagggg ccgagatacg cgccgctggc gaaaatctca tgatcccccg cgatgccctc
     3541 caccgcgttg tagatcgccg tttgcagcat cgccatgtcg cgcgcgattt ccggcgagag
     3601 cataccgctc tgtcgcatcg cctccagggc cgactcattc cagtccagca cccacgatga
     3661 cgcccgcgcc gctcccgcct tcaggagcag aaggcaaaaa agcagccgga tggcccggaa
     3721 agctcgaaaa ctggacgtca atgaagccat gaaacgcctg aacaatccct tcggaaggcc
     3781 gatattcaag cattatttat aaatgccata attcatccac acaatcctca accccgcgcg
     3841 caaccgaaat caccgagatt gcctttgacg aaagccacgc ggttgatgac actcgcaccg
     3901 taaacgacag gggtgtcccc gcgcagggac tgagacggac cgtccccggc ccgaaccctc
     3961 cgaacctgat gcgggtcatg ccgccgaagg gagctcgctc ggaggtatct cagttccggc
     4021 cacaggccgc cccaaccaaa aacgaagaac cacgaaccca gacctttccc cgacatgatc
     4081 gccgacccaa cgacctcctt tgagccgcat tcctccgagc agctccccgc ctccacccgc
     4141 gtctatgtcg aggggcagat ccacaaagac gtccgcgtcc ccatgcgcga gatcgctttg
     4201 agccccacga agagcttcaa cggccgcatc gaggtgaatg aacctgtgcg cgtctatgac
     4261 acctccggcc cttggggcga tcccagctac aaaggcaccg tcgaggaagg cctgcccgcc
     4321 ctgcgcaaag cctggatact ctcccgcaac gacgtcgagg aatacaccgg ccgcgccatc
     4381 gagccccgcg acaacggcta cctcaccgcc aaccacgccg aatacgccgc cgccaagcgc
     4441 gaaggcctcc tcagccccct caaggccccc atcaacgccc agcgcaatcc ccttcgcagc
     4501 accggcaagc ccgtcaccca gctccactac gcccgccagg gcatcatcac ccccgagatg
     4561 gagttcatcg ccatccgcga aaacatgcgc cgggcccaga tcgccgactt caaagacgac
     4621 atcctccgca acgacctcga caaacagcac gctggttccg caacactcca gcactccacc
     4681 actccatcca tcttccgccg cttcccccag cgcatccccg ccgaaatcac ccccgagttc
     4741 gtccgctccg aagtcgccgc cggccgcgcc atcatccccg cgaacatcaa ccaccccgag
     4801 ctcgagccga tgatcatcgg ccggaacttc ctcgtcaaaa tcaacgccaa catcggcaac
     4861 agcgccgtcg cctccagcat cgaggaagag gtcgaaaaaa tgcgctgggc caccaagtgg
     4921 ggcgcggaca ccgtcatgga cctctccacc ggcaagaaca ttcacgccac gcgcgagtgg
     4981 atcctgcgca acagccccgt gcccatcggc accgtgccca tctaccaggc gctcgaaaaa
     5041 gtgaacggca aggccgagga cctcacctgg gagctcttcc gcgacaccct catcgagcaa
     5101 gccgagcaag gcgtcgatta cttcaccatc cacgccggcg tcctcctccg cttcgtcccc
     5161 ctcaccgcct cccgcatgac cgggatagta agccgcggcg gcagcatcat ggccaaatgg
     5221 tgcctcgccc accacaaaga aaacttcctc tacacccact gggacgacat ctgcgacatc
     5281 atggccgcct acgacgtcag cttctccatc ggcgacggcc tccgccccgg ctccatcgcc
     5341 gacgccaacg acaaagccca gttcggcgaa ctcgaagtgc agggcgaact cacccaacgc
     5401 gcctgggcca aaggcgtcca agtcatgaac gaaggccccg gccacgtccc catgcacatg
     5461 atcgaggaaa acatggccaa gcagctcgaa tggtgccacg aagccccctt ctacaccctc
     5521 ggccctctca ccaccgacat cgcccccggc tacgaccaca tcacctccgg catcggtgcc
     5581 gccatgatcg gctggtatgg ctgcgccatg ctctgctacg tcacgcccaa ggaacacctc
     5641 ggcctcccca acaaagaaga cgtcaaagcc ggcgtcatca cctacaaact cgccgcccac
     5701 gccgccgacc tcgccaaagg ccaccccggc gcccaatacc gcgacaacgc cctctccaaa
     5761 gcccgcttcg agttccgctg ggaagaccag ttcaa
//
LOCUS       DAOMYJ010000278         5789 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Prosthecobacter sp. isolate AMR_MDS_5629
            NODE_4731_length_5789_cov_22.860481, whole genome shotgun sequence.
ACCESSION   DAOMYJ010000278 DAOMYJ010000000
VERSION     DAOMYJ010000278.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867850
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prosthecobacter sp. (wastewater metagenome)
  ORGANISM  Prosthecobacter sp.
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Verrucomicrobiaceae; Prosthecobacter.
REFERENCE   1  (bases 1 to 5789)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5789)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.30
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:34:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,742
            CDSs (total)                      :: 4,684
            Genes (coding)                    :: 4,676
            CDSs (with protein)               :: 4,676
            Genes (RNA)                       :: 58
            tRNAs                             :: 55
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5789
                     /organism="Prosthecobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4731_length_5789_cov_22.860481"
                     /isolate="AMR_MDS_5629"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1965333"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..861
                     /locus_tag="PLP58_19910"
     CDS             <1..861
                     /locus_tag="PLP58_19910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ10857.1"
                     /translation="SKTVQAPATLLNRASKAGGPVDQGFYTAALPAMEQDPVKPISEY
                     PQGTGYGSITLKNDGTLMLAGVLADGTKFTAASALVVDNTTPVYAQLLTPGTTTKEKG
                     GSLTGSLAFDPGAADSDVSAESLQWFRPAVSEQSGTTKAALATQLYTAGWPQGIHVRL
                     IGAKYDKTATAQTTLGLPEPGPWPGILSFDLGKLAAPVTATNFVINGNTVLISKTPGS
                     PFFTLGFTPSTGLMKGTFTPFWPSAKPPAFTGVLLGKGANQGGWGYFLSNQTGDTDPE
                     SGSVMLLGQP"
     gene            complement(899..1801)
                     /locus_tag="PLP58_19915"
     CDS             complement(899..1801)
                     /locus_tag="PLP58_19915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009959805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HRJ10858.1"
                     /translation="MPVSLDLHALARIRSLELRAKSVVEGLWRGLHRSPYHGFSVEFS
                     EYRAYVQGDDPRHMDWKVMARSDRAFIKKFEDETNLRCQLVVDHSRSMQFGSGPMTKA
                     DYAATLAATLALFLMQQGDAVGVTTFGQTLQEHIPPRNRPGHLRRLMLELEKPAPAGG
                     TALDLSVRQLADLLRKRGMICLVSDLLAPVDQLERQLGLLSAMGHDLVLFHVMDRAEI
                     DFTFEKAAHFRDAETGAERFVDPAVARAAYLQNLQTHRDHIRGSCERLGIEYHWTPTD
                     TPLEGVLFDFLSARSRKAGAVRRR"
     gene            2023..3723
                     /locus_tag="PLP58_19920"
     CDS             2023..3723
                     /locus_tag="PLP58_19920"
                     /inference="COORDINATES: protein motif:HMM:NF015684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LptF/LptG family permease"
                     /protein_id="HRJ10859.1"
                     /translation="MNLLQGARAGWARVRAWPYAGRSAFVLALLAMLLLAWEGHHREN
                     PADVAGYDVRGGSLIAADGAPAGPVLRAVSLLLPYLDQWMFVGGAVYVFILLRQWGNA
                     RKLVFPSWVAAPSVAAWAVCKDIALHFGPMQMTEMGEPPAMAAYWLKLGMVFVVALCP
                     AALLHFYTRQGALERYTLRTFFAPLVFCFIAFCSLWMIMDLLDNMKEFQDVGSSASTV
                     ALFYLSIIPFIYVSVMPAALLLAVLYTLTRMSRANEIVAMLGTGRSVVQILRPVLVSA
                     LALAAVSMAANYHWAPRAEGSRKAILRAMDERQKDSIRADVLMHRDPQTRRVWYIGTF
                     PFSLGESRLRGVQVREHDEAGHLTRVIHADSAIWRPDGVWRFFDGREVLHEKGEVAAI
                     RDFPEKDGNKMLVEKAFAETPWSMVSYALKADSMGVPELVSYIKTHAGDPPQKLRAFQ
                     AHYHHRFAMPWQSLALALVAAPLGIAWSRRGAVGGIAGSIFIFFGVLFLNNLCLNLAK
                     GGHAPAWLAAWIPHLIFGSLGLALLYYRSQNKDLPRLSLDFLFKRKPAPARPRRRAAA
                     "
     gene            3745..4329
                     /locus_tag="PLP58_19925"
     CDS             3745..4329
                     /locus_tag="PLP58_19925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ10860.1"
                     /translation="MIQNWSIRSRSHQCALTARPFEDGEVFHTAIYFDPETSGYLRRD
                     VALDAWAQETSERKPIAYWRTTYSPTIVEEKPEVASKESAMSLLQRFIEEDNPATENA
                     RYILALMLERKRILSPTASRETEDGRMLFYENKNTGEVFIIRDPELHLDEIAQVQDEV
                     ATLLGFGGPAAEAAKAAGMKITPEGKVEPAGEVA"
     gene            4402..5649
                     /locus_tag="PLP58_19930"
     CDS             4402..5649
                     /locus_tag="PLP58_19930"
                     /inference="COORDINATES: protein motif:HMM:NF024919.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C39 family peptidase"
                     /protein_id="HRJ10861.1"
                     /translation="MCKAPHLFLTCLLAGVTPGQAQEPPQLSADLDHLLNFAELWTST
                     RADFATRYTAKTDDETADKPPQFEWMSTEKDRARFSRKMFRNVETRLTMFGKSVKVEE
                     AVVEFVNDRPARVSVSFYNRGDSGAMETADFERIFKTVGQSLNQALKSAPRSVLNPSA
                     GASKLVSWGWNTPQVVALLEHNDYSPRANGGAAGQPEFLRLKLAAPSQADWTMGRLST
                     GVGRMGMAANVRKSADGDVHIANVPMVDQGDKGYCVAATCQRLFEYLRVPCDQHELAN
                     LVSVDAQSGVNIFSMQKSLKKVVGTYRVNFKTHINPELYYDNRRQRRVSLQEFTGIVK
                     EHIGKGVPLLWALELGKYKEDPPLPVSGQTIGGHMRMIIGYNEAKKHIIFSDTWGAGH
                     EIKRMDLPSAWECSIGLYSMTPR"
BASE COUNT          990 a         1897 c         1939 g          963 t
ORIGIN      
        1 agcaagaccg tgcaggcgcc cgccacgctg ctgaaccgcg ccagcaaggc cggcggcccg
       61 gtggaccagg gcttctacac agccgcgctg cccgccatgg agcaggaccc ggtgaaaccg
      121 atctcggagt atccgcaggg cacgggctac ggcagcatca cgctgaagaa cgacggcacg
      181 ctcatgctgg ccggggtgct ggcggacggg acgaagttca ccgccgccag cgcgctggtg
      241 gtggacaaca ccacgccagt ctatgcccaa ctgctgactc ccggcaccac cacaaaggaa
      301 aaaggcggca gcctcaccgg cagcctggcc tttgaccccg gcgcggcgga cagcgacgtg
      361 tccgccgaga gcctgcagtg gttccgcccg gcggtcagcg agcagtccgg cacgacaaag
      421 gccgcgctgg ccacgcagct ctacaccgcc ggctggccgc agggcatcca tgtgcggctc
      481 atcggggcga agtatgacaa gacggccacc gcgcagacga ccctgggcct gcctgaaccg
      541 ggtccctggc cgggcatcct gagctttgac ctgggcaagc tggcggctcc cgtgacggcg
      601 acgaatttcg tcatcaacgg caacacggtg ctcatcagca agactccggg atctccattc
      661 ttcaccctgg gcttcactcc aagcacgggc ctgatgaagg gaaccttcac acccttctgg
      721 ccgagcgcga agccgcccgc cttcaccggc gtgctgctgg gcaagggcgc caaccagggc
      781 ggctggggct atttcctgag caaccaaacc ggcgacaccg accccgaaag cggcagcgtc
      841 atgctgctcg gccagccgtg agcgcggggt gccgcgcccg cgagatcgtg gctttccctc
      901 acctcctcct caccgccccc gccttgcgcg agcgggcgga gaggaagtcg aagagcacgc
      961 cttccagcgg cgtgtccgtg ggggtccagt ggtattcgat gccgaggcgc tcgcaggagc
     1021 cgcggatgtg atcgcggtgt gtctggagat tctgcaaata ggcggcgcgc gccacggcgg
     1081 ggtccacgaa gcgctcggcg ccggtctcgg catcacggaa gtgggcggct ttctcgaagg
     1141 tgaagtctat ctcggcgcgg tccatgacgt ggaagagcac gaggtcgtgc cccatggcgc
     1201 tgagcaggcc gagctggcgc tcgagctggt ccacgggggc gagcaggtcg gagacgaggc
     1261 agatcatgcc gcgcttgcgc aggaggtcgg cgagctggcg cacgctgagg tcgagcgcgg
     1321 tgcctccggc gggcgcgggc ttttccagct cgagcatgag gcggcgcagg tggccggggc
     1381 ggttgcgcgg cgggatgtgc tcctggaggg tctggccgaa agtggtgacg ccgaccgcgt
     1441 ccccctgctg catgaggaag agggcgaggg tggcggcgag ggtggcggcg tagtcggcct
     1501 tggtcatggg gccggagccg aactgcatgg agcggctgtg gtccaccacg agctggcagc
     1561 gcaggttggt ctcgtcctcg aattttttga tgaaggcgcg gtcactgcgg gccatgacct
     1621 tccagtccat gtggcgcggg tcatccccct ggacataggc gcggtattcg gagaactcga
     1681 cggagaagcc gtggtagggg ctgcgatgca ggccgcgcca gaggccctcg acgacgcttt
     1741 tggcgcgcag ctcaagagag cggatgcggg ccagggcatg gagatcgagg gagacgggca
     1801 ctggatggaa aatggcggat gaggggcgcg cgttcagccg cgccgccgac acaacgtccg
     1861 cgcgccgggc ttcatgtcag ggatggatca agaaagtgtc cgggcaagtg ccggaccggt
     1921 ttgggttttt cctgatgcgg tctcaaccgc cggggtcttc ccgcattgac tccgccgggc
     1981 ttttcgccaa ccctgcgcgc ccctggaatg atgaaccggt tcatgaacct gctgcaaggc
     2041 gcccgcgctg gctgggcgag ggtgcgcgca tggccttacg cggggcggtc cgcctttgtg
     2101 ctggcactgc tggccatgct gctgctggca tgggagggac atcaccggga gaaccctgcg
     2161 gatgtggcgg gttatgatgt gcgcgggggc agcctgatcg ccgctgacgg ggctcccgct
     2221 gggccggtgc tccgcgcggt ctcgctgctg ctgccttatc tggaccagtg gatgttcgtc
     2281 ggcggggcgg tgtatgtttt catcctgctg cggcagtggg ggaacgcgcg caagctggtc
     2341 ttcccctcct gggtggccgc gccgtccgtg gcggcgtggg cggtgtgcaa ggacatcgcc
     2401 ctgcatttcg gcccgatgca gatgacggaa atgggcgagc ctccggccat ggcggcctac
     2461 tggctgaagc tgggcatggt cttcgtggtg gcactgtgcc cggcggcgct gctgcatttc
     2521 tacacccgtc agggcgcgct tgagcgctac acgctgcgca cgttcttcgc gccgctggtc
     2581 ttttgcttca tcgccttctg ctcgctgtgg atgatcatgg acctgctgga caacatgaag
     2641 gaattccagg acgtgggcag cagcgcctcc acggtggcgc tcttttacct gagcatcatc
     2701 cccttcatct acgtctccgt catgcccgcc gcgctgctgc tggcggtgct ctacacgctg
     2761 acgcgcatgt cacgcgccaa cgagatcgtg gccatgctcg gcacgggccg cagtgtcgtg
     2821 caaatcctgc gtcctgtgct cgtctcagcg ctggccctgg ccgcggtcag catggccgcc
     2881 aactaccact gggcgccgcg cgccgaaggc agccggaagg ccatcctgcg cgccatggac
     2941 gagaggcaga aggactccat ccgcgcggat gtgctcatgc accgcgaccc tcagacccgc
     3001 cgtgtctggt acatcggcac cttccccttt tccctcggag agagccgcct gcgcggcgtg
     3061 caggtgcgcg agcacgacga ggcgggacac ctgacgcgcg tcatccacgc cgactcggcc
     3121 atctggcgcc cggatggtgt gtggcgtttc tttgacggca gggaggtgct gcatgaaaag
     3181 ggcgaggtgg ccgccattcg cgacttcccg gaaaaggacg gaaacaaaat gctggtggag
     3241 aaagccttcg ccgagacgcc ctggagcatg gtcagctacg cgctcaaggc cgactccatg
     3301 ggcgtgccgg aactggtctc ctacatcaag acacatgccg gcgacccgcc tcagaagctg
     3361 cgcgccttcc aggcgcatta ccaccaccgc tttgccatgc cttggcaaag cctggccctt
     3421 gctcttgtcg ccgcgccgct tggcatcgcc tggtcgcggc gcggagcggt gggcggcatc
     3481 gccggctcca tcttcatttt cttcggcgtg cttttcctca acaacctttg cctgaacctg
     3541 gccaagggcg gccacgcacc cgcatggctg gcggcgtgga ttccgcatct catcttcggc
     3601 tcgctgggtc tggcgctgct ttactaccgc tcgcaaaaca aggatctgcc gcgcctcagc
     3661 ctggacttcc tgttcaaacg caagcccgcg cccgcccggc cgcgccgcag ggctgccgcc
     3721 tgaccccgct ttatccgacc gcccatgatt caaaactggt ccattcgctc ccgctcccat
     3781 cagtgcgccc tgaccgcgcg tcctttcgag gacggggagg tctttcacac cgccatctac
     3841 ttcgaccccg aaaccagcgg ctacctccgg cgcgatgtgg cgctcgacgc ctgggcacag
     3901 gaaaccagcg agcgcaaacc catcgcctac tggcgcacga cttattcgcc gacgatcgtc
     3961 gaggaaaagc ccgaggtcgc cagcaaggag agcgccatga gcctgctcca gcgcttcatc
     4021 gaggaggaca atcccgccac cgaaaacgcg cgctacatcc tggccctcat gctcgagcgc
     4081 aagcgcatcc tcagccccac cgcgtccagg gagaccgagg acggcaggat gctcttttac
     4141 gagaacaaaa acaccggcga ggtcttcatc atccgcgacc ccgagctgca tctcgacgag
     4201 atcgcccagg tgcaggacga ggtggccacc ctgctcggct tcggcggccc cgccgccgag
     4261 gctgccaaag ccgccggcat gaagatcacc ccggagggca aagtggagcc ggccggggaa
     4321 gtggcttgag tcgcctccat cccgcttggc gggaattttg tttgttgacc tgtgtgccgg
     4381 cgttcgcgca gaatgcgcgg catgtgcaaa gcaccgcatc tctttctgac atgtctgctg
     4441 gccggagtga cgcccggcca ggcccaggag ccgccgcagc tctccgccga ccttgatcat
     4501 ctgctgaact tcgcggaact gtggacctcg acgcgcgcgg atttcgcgac ccgctacaca
     4561 gcgaagacag acgatgaaac ggcggacaag ccgccgcagt tcgagtggat gagcacggag
     4621 aaggaccgcg cgcgcttcag ccgcaaaatg ttccgcaacg tggaaacgcg cctgaccatg
     4681 ttcggcaagt cggtgaaagt ggaggaggcg gtggtcgagt ttgtcaatga ccgcccggca
     4741 agagtgagcg tgtcctttta caaccgcggc gactccggcg cgatggagac ggcggacttc
     4801 gagcgcatct tcaagaccgt gggccagagc ctcaaccagg cgctgaaatc ggcgccgcgc
     4861 agcgtcctga atccctccgc cggagcctcg aagctggttt cctggggctg gaacacgccg
     4921 caggtggtgg cgctgctgga gcacaacgat tactcgccgc gcgccaatgg cggcgctgcc
     4981 gggcagccgg agttcctgcg cctgaaactc gccgcgccga gccaggcgga ctggaccatg
     5041 ggccgcctct ccaccggcgt gggccgcatg ggcatggcgg ccaacgtgcg caagtcggcc
     5101 gacggcgacg tccacatcgc caacgtgccg atggtggacc agggtgacaa aggctactgc
     5161 gtggcggcca cctgccagcg cctctttgaa tatctgcgcg tgccgtgcga ccagcatgag
     5221 ctggcgaatc tcgtcagcgt ggacgcgcag agcggcgtca acatcttctc catgcagaag
     5281 agcctcaaga aagtcgtcgg cacctacagg gtgaacttta aaacgcacat caacccggag
     5341 ctttattacg acaaccgccg ccagcgccgc gtgtccctcc aggagttcac cggcattgtc
     5401 aaagagcata ttggcaaggg cgtgcccctg ctctgggcgt tggaactcgg caaatacaag
     5461 gaggatccgc ccctgccggt cagcggccag accatcggcg gccacatgcg catgatcatc
     5521 ggctacaacg aggcgaagaa gcacatcatc ttctccgaca cctggggcgc cgggcacgag
     5581 atcaagcgca tggatctgcc ctccgcgtgg gaatgcagca tcggcctcta cagcatgact
     5641 ccgcgctgag gcgcgcaggc gcagccggcg gaggcatgct ccgcattcac ggattcttcg
     5701 cgggttcatt ccgccccgat tccagtctgg cggcaggcgc cgcaggctgt gggaggctgg
     5761 gcgcgggagg gcgcgccagc ggcgcgggc
//
LOCUS       DAOMYJ010000279         5755 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Prosthecobacter sp. isolate AMR_MDS_5629
            NODE_4770_length_5755_cov_18.966667, whole genome shotgun sequence.
ACCESSION   DAOMYJ010000279 DAOMYJ010000000
VERSION     DAOMYJ010000279.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867850
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prosthecobacter sp. (wastewater metagenome)
  ORGANISM  Prosthecobacter sp.
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Verrucomicrobiaceae; Prosthecobacter.
REFERENCE   1  (bases 1 to 5755)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5755)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.30
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:34:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,742
            CDSs (total)                      :: 4,684
            Genes (coding)                    :: 4,676
            CDSs (with protein)               :: 4,676
            Genes (RNA)                       :: 58
            tRNAs                             :: 55
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5755
                     /organism="Prosthecobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4770_length_5755_cov_18.966667"
                     /isolate="AMR_MDS_5629"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1965333"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            371..1849
                     /locus_tag="PLP58_19935"
     CDS             371..1849
                     /locus_tag="PLP58_19935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007415763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1501 domain-containing protein"
                     /protein_id="HRJ10862.1"
                     /translation="MHPVEQQILQTRREFLATSANGIGALALGAMLTREGVISAAPPV
                     ADPLAPRAPHFPARARNCIFIFMEGAPSQMDLFDPKPKLNELHGQALPESMTKNVRFA
                     FIKKETARLMGSPRKFQKHGQCGMDFSDLLPHMAKTADEWLMIRSLHTEQFNHHPGQL
                     VLHSGRAMFGLPTVGSWLNYGLGSPSGNLPGYVVLSAGRGTSGGASLWQSGFLPSSYA
                     GVLFRNQGEPVLNLKNPPGIPMSLQRKGLDALMELNQSSFSHFNDPEIASRIAAYELA
                     FRMQSAAPELIDLSGEDARTLEMYGVNRPDPPIKAQRGGGPGQYKAFATNCLLARRMV
                     ERGVRFVSIMHASWDHHSNLDSELAYNAGMADQPIAALIKDLKQRGLLEDTMVVWGSE
                     FGRTPLGENRNGSPNVTGRDHHPFAFTMLMAGGGVKGGRVYGETDEIGWSIVRDPVDL
                     HDFHATLLHQFGFDHLRLTHRFQGRDFRLTDVSGRVIKEWIA"
     gene            complement(1859..2605)
                     /gene="fabG"
                     /locus_tag="PLP58_19940"
     CDS             complement(1859..2605)
                     /gene="fabG"
                     /locus_tag="PLP58_19940"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009963251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004316 - 3-oxoacyl-[acyl-carrier-protein]
                     reductase (NADPH) activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] reductase"
                     /protein_id="HRJ10863.1"
                     /translation="MGKLQDKVAVITGAGRGIGRAIAETFAAEGAKVAVVSRTEASSQ
                     AAAEAINAAHPGAAKAYAVDVADAAATAELGKAVLADFDHVDILVNNAGVTKDGLLLR
                     MSDADWDFVVDTNLKGAFNMVKAVQRSILKQKGARIINVASVIGLIGNAGQANYAASK
                     AGLIGFTKSLAREFAGRGVTANVIAPGFIATDMTGVLDEKVKAAILEKIPLGDFGQGA
                     DIAAAALFLASPEARYITGQVLAVDGGMVM"
     gene            complement(2657..4375)
                     /locus_tag="PLP58_19945"
     CDS             complement(2657..4375)
                     /locus_tag="PLP58_19945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009963789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ10864.1"
                     /translation="MKELLLIDAIGPFFRGYDRRVINWSKIPWDHAGRQGAAWWENAR
                     SELNVLARQAVAWGFNAASIDDVAHLADHEWLEPEVRQRVGRYRTEMRRCLEVLTRTG
                     LAPHVTMDVFSTTPGLQRRLRIERMEPGEYLRGLLDRFLTDFPDVAGVIVRIGESDGK
                     DVHDDFRSQLVLKTPEQARAFLQEMLPVFERHGRRLIFRTWTVGAYPIGDLMWHRDTF
                     ARVFEGITSPALVVSMKYGESDFFRYLPLNRNFFRTPLAKIVELQSRREYEGCGEYPN
                     FTGWLHERFARELRQAQNVIGCMVWCQTGGWVPFRRLSFLDPEALWNDLNTYVTIRVV
                     REGCLAEEAVRGFARERRLGDADALIELLRLADECVRELLYVEEFARQKLFFRRVRIP
                     PMLVVYWGNIFISHGLRRLLRYFVKDADAALRSARRCMDNLERMFPLAEKAGVPLEDI
                     EYMRDTFHLLALAREYYFADDAPDIEQRILEAKRAYKRKYAKRGLRARYRVKTDFKPL
                     LLNPRHLGWAVSFLMRRQRGYRMVDRLVILHGLSLIYRVIAARRPHWIPGFASKSAMG
                     VDVVFK"
     gene            complement(4442..5419)
                     /locus_tag="PLP58_19950"
     CDS             complement(4442..5419)
                     /locus_tag="PLP58_19950"
                     /inference="COORDINATES: protein motif:HMM:NF025465.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PmoA family protein"
                     /protein_id="HRJ10865.1"
                     /translation="MRLLFLLLLPCPALTAQVSPLPSAKPVPRMQAVPLPHHITSFQL
                     DGRELTACHFDPADMRPFWHPIRTTKEASITRMGHPHDPHGHSHHNSVWITHNLLNGV
                     DFWGDHAREQGRIVCAQVPREAYEEGDEAASMRMVNHWIKVADGSVQLVETRRSEVRP
                     LDGARSWFMIVDLELGAPKGGKAVFGATGFGLIAVRMARSIGVHDGGGRILNSEGLVN
                     EKAVFRQPARWCDYSGRLTNEDDGFAGITLMNHPMNPRHPTAFHVRDDGWMGCCLSLD
                     EDVEIAAGGSLRLRYGLWVHDGVSTLEESEAHWRRFAELPVADLGGKKP"
     gene            5510..>5755
                     /locus_tag="PLP58_19955"
     CDS             5510..>5755
                     /locus_tag="PLP58_19955"
                     /EC_number="1.1.1.1"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HRJ10866.1"
                     /translation="MNFEFATATRIVFGPGSARRLGELCAGLAQRFLVVTGRDTARHA
                     PVLEGLKRAGCRLIPLLTQGEPTLLDARNGASFAREAG"
BASE COUNT          925 a         2062 c         1864 g          904 t
ORIGIN      
        1 cgcggcgcgc cgcctcccca aagccgggcg ggctgcgcca cacccggcct gcggcatccc
       61 ggcggcgcac ccgtctaggt gcgggtctcc gagcaaagcg ggacactcca tcccgcagcg
      121 cgcgcgggca tgacacaggt cttgggcgcg accttgacga agttcgccga cttgcgcgcg
      181 caccggcggc ggggtgccgg tgggttcaag gctgcggcgg cggcatgggc gcgggcatca
      241 atgacggcgg ctgccttttt gccgccctcc ccccccggcg gcggcaacga tgcggcgcgg
      301 gtcttcgcgt ggcggagcgc ccgccctgca acagcgcggc cccgcgccgc gttctgtggg
      361 tgtgatctcc atgcaccccg tcgagcagca aatcctccag acccgccgcg agttcctcgc
      421 caccagcgcc aacggcatcg gcgccctggc gctcggggcc atgctgacgc gggagggcgt
      481 catttccgcc gccccacccg tggcggaccc cctggcgccg cgcgcgccgc atttccccgc
      541 cagggccagg aactgcatct tcatcttcat ggagggcgcg ccctcgcaga tggacctctt
      601 cgacccgaag ccgaagctca acgagctgca cggccaggcg ctgccggaga gcatgacgaa
      661 gaacgtgcgc ttcgccttca tcaaaaagga gaccgcgcgg ctcatgggca gcccgaggaa
      721 gttccagaag cacggccagt gcggcatgga cttcagcgac ctgctgccgc acatggcaaa
      781 gacggcggac gagtggctca tgatccgcag cctgcacacc gagcagttca accaccaccc
      841 cggccagctc gtgctgcaca gcgggcgcgc catgttcggc ctgccgacgg tgggctcctg
      901 gctgaactac ggcctgggca gcccctccgg caacctgccg ggctatgtgg tgctcagcgc
      961 cgggcgcggc accagcggcg gcgcgtccct gtggcagagc ggcttcctgc ccagctcgta
     1021 tgccggggtg ctcttccgca accaggggga gccggtgctc aacctgaaaa acccgcccgg
     1081 catccccatg agcctccagc gcaagggcct ggacgcgctc atggagctga accagtcctc
     1141 cttttcccac ttcaacgacc ccgagatcgc cagccgcatc gccgcctacg agctggcctt
     1201 ccgcatgcag tccgccgcgc cggagctgat agacctctcc ggcgaggacg ccaggacgct
     1261 ggagatgtat ggcgtcaacc gccctgaccc gcccatcaaa gcccagcgcg gcggcgggcc
     1321 ggggcagtac aaagccttcg ccaccaactg cctgctggcg cgccgcatgg tggagcgcgg
     1381 cgtgcgcttc gtcagcatca tgcacgcgag ttgggaccac cactcgaacc tggacagcga
     1441 actggcctac aatgccggca tggccgacca gcccatcgcc gcgctgatca aggacctcaa
     1501 acagcgcggc ctgctggagg acacgatggt ggtctggggc agcgagttcg gccgcacgcc
     1561 gctgggggaa aaccgcaacg gcagtcccaa cgtcaccggc cgcgaccacc accccttcgc
     1621 cttcaccatg ctcatggcgg gcggcggcgt caaaggcggc cgggtttacg gcgagaccga
     1681 cgagatcggc tggagcatcg tccgcgaccc cgtggacctg cacgacttcc acgccacgct
     1741 gctgcaccag ttcggcttcg accacctgcg cctcacgcac cgcttccaag gccgcgactt
     1801 ccgcctcacg gatgtctccg ggcgcgtgat caaagaatgg attgcctgag tgcggcggtc
     1861 acatgaccat gccgccatcc acggccagga cctggccggt gatgtaacgc gcctccgggc
     1921 tggcgaggaa gagcgcggcg gcggcgatgt cggctccctg gccgaagtcg ccgaggggga
     1981 tcttctccag gatggcggct ttgactttct cgtcgaggac tccggtcatg tcggtggcga
     2041 tgaagccggg ggcgatgaca ttggcggtga caccccggcc tgcgaactcg cgggcgaggg
     2101 acttggtgaa gccgatgagg cccgccttgc tggcggcgta gttggcctgg cccgcgttgc
     2161 cgatgaggcc gatgacggag gcgacgttga tgatgcgcgc gcccttctgc ttgaggatgc
     2221 tgcgctggac ggctttgacc atgttgaagg cgcccttgag gttcgtgtcc accacgaagt
     2281 cccagtccgc gtcactcatg cgcaggagca ggccgtcctt ggtgacgccg gcgttgttga
     2341 ccaggatgtc cacatggtcg aaatcggcga gcacggcctt gcccagctcg gcggtggcgg
     2401 cggcgtccgc gacatccaca gcgtaggctt ttgccgcgcc ggggtgcgcc gcgttgatgg
     2461 cctccgccgc ggcctgagag ctggcctcgg tgcggctgac cacggcgact ttggcgccct
     2521 cggcggcgaa ggtttcggcg atggcgcggc cgatgccgcg gcccgccccg gtgatgacgg
     2581 cgactttgtc ctggagtttt cccatgcgcc atgatgccgg gcggggcagg ggagtcaagg
     2641 cgcgggtctg cgcggattac ttgaagacca catccacacc catcgcgctc ttcgaggcga
     2701 agccggggat ccagtgcggg cggcgcgcgg cgatgacgcg gtagatcaag gacaacccgt
     2761 gcaggatgac cagccggtcc accatgcggt agccgcgctg gcggcgcatg aggaagctga
     2821 ccgcccagcc gaggtggcgc ggattgagca gcagcggctt gaagtccgtc ttcacccggt
     2881 agcgcgcgcg taggccgcgc ttggcatact tccgcttgta ggcgcgcttg gcctccagga
     2941 tgcgctgctc aatgtccggc gcgtcatcgg cgaagtagta ttcccgggcc agcgccagca
     3001 ggtggaaggt gtcccgcatg tattcgatgt cctccagcgg cacgccggcc ttttccgcca
     3061 ggggaaacat gcgctccagg ttatccatgc accggcgcgc gctgcgcagg gccgcgtcgg
     3121 cgtccttcac gaagtagcgc agcagccggc gcaggccgtg gctgatgaag atgttgcccc
     3181 aatagaccac cagcatcggc gggatgcgca cccggcggaa gaagagcttc tgccgggcaa
     3241 actcctccac atacagcagc tcgcgcacgc actcgtcggc caggcgcagc agctcgatga
     3301 gggcgtccgc gtcgccgaga cggcgctctc tggcaaagcc gcgcacggct tcctcggcca
     3361 ggcagccctc cctcaccacg cggatcgtca cataggtgtt gaggtcgttc cacagcgcct
     3421 cggggtccag gaagctcagc ctgcggaagg gcacccagcc gcccgtctgg caccagacca
     3481 tgcagccgat cacattctgc gcctggcgca gctcgcgggc gaagcgctcg tgcagccagc
     3541 cggtgaagtt cgggtattcc ccgcagccct cgtattcgcg gcgcgactgc aactcgacga
     3601 tcttcgccag cggcgtgcgg aaaaagttcc ggttcagcgg caggtagcgg aagaagtccg
     3661 actcgccgta cttcatcgag accaccaatg ccggactggt gatgccttcg aacactctgg
     3721 caaaggtgtc gcggtgccac atcaggtccc cgatggggta ggcgcccacc gtccaggtgc
     3781 ggaagatgag cctccttccg tgcctctcga agacgggcag catctcctgg aggaaggccc
     3841 gcgcctgctc cggggtcttg agcacgagtt ggctgcggaa gtcgtcatgc acgtccttgc
     3901 cgtcgctctc gccgatgcgc acgatgacac ccgccacatc ggggaaatcg gtgaggaacc
     3961 ggtccagcag tccgcgcaga tactcgcccg gctccatgcg ctcgatgcgc agccgccttt
     4021 gcagccccgg cgtggtggaa aagacatcca tcgtcacatg cggggccagc cccgtgcgtg
     4081 tcagcacctc caggcagcgg cgcatctccg tgcggtagcg gcccacgcgc tgccgcacct
     4141 ccggctccag ccactcatgg tccgccaggt gcgccacatc atctatggat gccgcgttaa
     4201 agccccacgc caccgcctgc cgcgccagca cgttcagctc cgagcgcgcg ttctcccacc
     4261 aggccgcgcc ctgccgtccc gcgtgatccc aggggatctt cgaccagttg atgacacgcc
     4321 ggtcgtagcc gcggaaaaac ggcccgatcg cgtctatgag caacagttcc ttcactcggc
     4381 acagatgacg agccatcctg gccgtgcgat gcggcgcgcg tgacgctttt gtcacataaa
     4441 ctcagggctt cttcccgccc aggtctgcca ccggcagctc cgcgaacctc cgccagtgcg
     4501 cttcgctctc ctccagtgtg ctcaccccgt catgcaccca caagccgtag cgcaggcgca
     4561 ggctgccgcc tgcggcgatc tcaacatctt catccaggct caggcagcag cccatccagc
     4621 cgtcatcgcg cacatggaag gcggtgggat ggcgcgggtt catcggatgg ttcatcagcg
     4681 tgatgcccgc gaacccgtca tcctcgttgg tcagccttcc gctgtagtcg caccagcgcg
     4741 ccggctggcg gaacaccgcc ttctcattga ccagcccctc ggagttcagg atgcgcccgc
     4801 cgccgtcatg cacgccgatg ctgcgagcca tgcgcaccgc gatcaagccg aagcccgtgg
     4861 cgccaaacac cgccttcccg cccttcggcg cgcccagctc caggtccacg atcatgaacc
     4921 agctccgcgc cccgtccagc gggcgcacct ccgagcgccg cgtctccacc aactgcacgg
     4981 agccgtccgc caccttgatc cagtggttca ccatgcgcat cgaggccgcc tcatccccct
     5041 cctcatacgc ctccctcggc acctgcgcgc agacgatgcg gccctgctcc ctcgcatggt
     5101 cgccccagaa atccaccccg ttgagcaggt tgtgggtgat ccacacactg ttgtggtggc
     5161 tgtgcccgtg cgggtcatgc gggtggccca tgcgcgtgat gctggcctcc ttggtcgtgc
     5221 gtattggatg ccagaacggt cgcatgtccg ccggatcgaa gtggcaggcc gtcagctccc
     5281 gcccgtcgag ctggaaggac gtgatgtgat gcggcagcgg caccgcctgc atgcgcggca
     5341 ccggcttggc cgagggcagg ggggagacct gcgcggtgag cgcggggcag gggagcagga
     5401 ggaggaaaag caggcgcatg accggcagga tacgctccct ggagcgccat tcatccgccg
     5461 caggcaaact cccccgtgca aaacaccccg tcccgcccat cctgccccca tgaacttcga
     5521 gttcgccacc gccacccgca tcgtcttcgg ccccggcagc gcccgccgcc tgggggagtt
     5581 gtgcgccggc ctcgcccagc gctttctcgt cgtcaccggg cgcgacaccg cccgccacgc
     5641 gcctgtcctg gagggcctca agcgcgccgg ttgccgcctc attcccctgc tcacccaggg
     5701 cgagcccacc ctcctggatg cccgcaacgg cgccagcttc gcgcgagagg ccgga
//
LOCUS       DAOMYJ010000280         5737 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Prosthecobacter sp. isolate AMR_MDS_5629
            NODE_4788_length_5737_cov_20.167899, whole genome shotgun sequence.
ACCESSION   DAOMYJ010000280 DAOMYJ010000000
VERSION     DAOMYJ010000280.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867850
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prosthecobacter sp. (wastewater metagenome)
  ORGANISM  Prosthecobacter sp.
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Verrucomicrobiaceae; Prosthecobacter.
REFERENCE   1  (bases 1 to 5737)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5737)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.30
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:34:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,742
            CDSs (total)                      :: 4,684
            Genes (coding)                    :: 4,676
            CDSs (with protein)               :: 4,676
            Genes (RNA)                       :: 58
            tRNAs                             :: 55
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5737
                     /organism="Prosthecobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4788_length_5737_cov_20.167899"
                     /isolate="AMR_MDS_5629"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1965333"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..184
                     /locus_tag="PLP58_19960"
     CDS             <1..184
                     /locus_tag="PLP58_19960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009962935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ10867.1"
                     /translation="EAGDGWQRLVLRVEPRQDVREAVMALASERGWQVRELHRRLPGL
                     EEVFVELASAESPGVF"
     gene            194..952
                     /locus_tag="PLP58_19965"
     CDS             194..952
                     /locus_tag="PLP58_19965"
                     /inference="COORDINATES: protein motif:HMM:NF024141.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRJ10868.1"
                     /translation="MRIFWVLLKKELRAFFVSPMAYIVLALVMVLNGFCLRAALSVLE
                     RGPSEGSIVTWTFHAMWFWLSYFFIFPLLTMRLFAEEKKMGTLETLFTAPVRTWQVVW
                     SKYAASVLLYCLLWTPSALNFALVDWITAGQVEMPAGALKGSYLILFVMGLFNLAIGC
                     FASSLTSNQIIAAVVSFTMSLLHFLGGLFISVIGRQVSESFVDIIHYIASTEHIRIFT
                     NGLIDTRPLVYYTSFALLFLALTHQVVESRRWKP"
     gene            complement(963..2939)
                     /locus_tag="PLP58_19970"
     CDS             complement(963..2939)
                     /locus_tag="PLP58_19970"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HRJ10869.1"
                     /translation="MNSLRLRFLLPLVLFGFLPAMGAARLMGHSLDWFFGTQGVLLGG
                     GVFLAAYLLSGWVLKPVAAMMNATASLMRGIPPTKDVAEWLPADFWKLRCDVNMLFAK
                     HRQALSTAETHSCQTAQRLANAERLLRDAFTALQGIFSASDEGVAVVDGNGTIIASNA
                     RLDEFLGSPLDENGGRDFETLAGLLAARFQDSGKVAGWIRECLEEPAFKGSLEAGMDG
                     GAIMELRTAPMLGDSGEVLGRLWLLRDRTELSGLRQQLRESQKLGTLGQLAGGIAHDF
                     NNMLTTIRGNLTLAEISPGGDVKEKIKTATHATQRAADLVKQLLSYSRRSGGASARRI
                     TNVKKLLADVENMLKHSVDPRVSIRVRAGMDASFVMADAAQLEQVVLNLCINARDSLP
                     AENGVIEIGIENITQRAPDGVSGPAEFTVITVRDNGRGIPESVRPHIFEPFFTTKQDG
                     KGTGLGLSMAQDIIREHGGWIEFDSAEGQGSVFRIHLPRTSDPENVDDDLHADALIPA
                     QSLESGRGTILVVDDEESVRATAVNMLKYLGYQILEAADGQQALDVLLHSGRQVDAVM
                     LDIYMPVLSGRDAFKQMREAGNDTPVVICSGFVMDPDEFVILGQGHPPPVEIMLKPYS
                     LDALGKAMAKAVQSGREAAPPAGRSAARIPFFQH"
     gene            complement(2936..3415)
                     /locus_tag="PLP58_19975"
     CDS             complement(2936..3415)
                     /locus_tag="PLP58_19975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ10870.1"
                     /translation="MPRTAIAASLASAAKTPGELLPDPGADKTAVPAVDSWKTEIDEG
                     INQLRNDIFAAVMGVSALKDRLDRLEYHAPAPSQPANDLPEAAREQILAWIEDRLASC
                     LDEKIACALDQVISARQESLITRMSTAEFFRQPPGFPLPDRRTAFAQAPTILATSLS"
     gene            3864..>5737
                     /locus_tag="PLP58_19980"
     CDS             3864..>5737
                     /locus_tag="PLP58_19980"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018968975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HRJ10871.1"
                     /translation="MPPAHNYTEDSIKSLDWREHIRLRPGMYIGKLGDGSQPEDGIYV
                     LLKEVLDNSIDEHVMGYGKVIEVEITEDHQVTVRDYGRGIPLGKLMECAAQINTGAKY
                     DSEVFKRSVGLNGVGIKAVNALSTFFEIQAIREKQTRAIEFSQGIVKYDMGRPAPCDE
                     PDGTRITFRPDTESFTSHVHFKLDYVREMLRFYSWLNPGLTLKLNGETFRSKDGLRDL
                     IAAKLTEEPLYPVVYLTGTDIEVAFTHGTGYGEEYYTFVNGQHTTQGGTHLAAFREAI
                     VQECRAYFNKQYDPNDVRGSLIAAVSVKVQEPVFESQTKTKLGSTHMEPNGRNLRTHI
                     INTVVKELDNYLHKHGDLAKALQEKINANEKERKEISGIQKAARDSARKAKVHNKKLR
                     DCRIHLDTKDKRREESTLFITEGDSASGSITKSRDVETQAVFSLRGKPLNSYGLSRKI
                     VYENEEFYLLANALQIDENIEDLRYNKIILATDADVDGMHIRLLMITFFLQFFPEIVR
                     AGHLHILQTPLFRVRNKKETFYCYGDEERQRAIHCCGKNAEITRFKGLGEISPDEFKH
                     FIGPKMRLEPVMMPEHKTIKELLAFYMGKNTPDRQTYIVDNLRVEQELRFTGEGGGGE
                     "
BASE COUNT         1087 a         1622 c         1911 g         1117 t
ORIGIN      
        1 ggaggcgggg gacggctggc aaaggctggt gctgcgcgtg gagccgcgcc aggatgtgcg
       61 cgaggcggtg atggcgctgg cctcggagcg cggctggcag gtgcgggagc tgcaccgcag
      121 gctgccgggg ctggaggagg tgtttgtgga actggcgtcg gcggagtcgc cgggcgtgtt
      181 ttaattgatc atcatgagaa ttttctgggt gctgctgaaa aaggaactgc gggcgttttt
      241 cgtctcgccg atggcctaca tcgttctggc gctggtgatg gtgctcaatg gcttctgcct
      301 gcgcgcggcg ctctcggtgc tggagcgcgg tccgagcgag gggtccatcg tgacgtggac
      361 cttccatgcg atgtggttct ggctttcgta tttcttcatc ttcccgctgc tgacgatgcg
      421 gctgttcgcg gaggagaaaa agatggggac gctggagacg ctgttcacgg cgccggtgcg
      481 cacctggcag gtggtgtggt cgaagtacgc ggcctcggtg ctgctctact gcctgttgtg
      541 gacgccgagc gcgctgaact tcgcgctggt ggactggatc accgcagggc aggttgagat
      601 gccggcgggg gcgctgaagg gcagctacct gattttgttt gtgatggggc ttttcaacct
      661 ggcgatcggc tgcttcgcct cctcgctgac ctcgaaccag atcatcgcgg cggtggtgtc
      721 cttcacgatg agcctgctgc atttcctggg cgggctgttc atcagcgtca tcggccggca
      781 ggtgagcgag agctttgtgg acatcatcca ctacatcgca agcacggagc acatccgcat
      841 cttcaccaac gggctgattg acacgcgccc gctggtttac tacacgagct tcgcgctgct
      901 gttcctggcg ctgacgcacc aggtggtgga gtcccgccgg tggaagccct gagcacgggc
      961 ggctagtgtt ggaagaaggg gatccgggcg gcgctgcggc cggccggggg ggcggcctcc
     1021 ctgcctgact ggacggcttt ggccatggcc ttgccgaggg cgtcgagaga gtagggtttg
     1081 agcatgattt cgacgggcgg cgggtgcccc tggccgagaa tgacgaactc gtcggggtcc
     1141 atgacgaagc cgctgcagat gacgacgggg gtgtcgttgc cggcctcgcg catttgcttg
     1201 aaggcgtcgc gcccgctgag cacgggcatg tagatgtcga gcatgacggc gtccacctgg
     1261 cgtccgctgt ggaggaggac atcaagcgcc tgctggccgt cggcggcttc gagtatctgg
     1321 tagccgaggt atttgagcat gttcacggcg gtggcgcgca cggattcctc gtcgtccacg
     1381 acgaggatgg tgccgcgccc gctctcgagg gactgggcgg ggatgagggc gtccgcgtgg
     1441 aggtcgtcat ccacattttc ggggtcgctg gtgcggggca gatggatgcg gaagacgctg
     1501 ccctggccct cggcgctgtc gaactcgatc cagccgccgt gctcgcggat gatgtcctgc
     1561 gccatggaga gtccgagtcc ggtgcctttg ccgtcctgtt tggtggtgaa gaagggctcg
     1621 aagatgtgcg ggcggacgga ttcgggaatg ccgcggccgt tgtcgcgcac ggtgatgacg
     1681 gtgaactcgg cggggccgga gacgccgtcg ggagcgcgct gggtgatgtt ttcgatgccg
     1741 atttcgatga cgccgttctc ggcgggcagg gaatcgcgcg cgttgatgca gagattgagg
     1801 acgacctgct cgagctgggc ggcatccgcc atgacgaagg aggcgtccat gccggcgcgg
     1861 acgcggatgc tgacgcgggg gtccacgctg tgcttgagca tgttttcgac gtcggcgagg
     1921 agctttttga cgttggtgat cctgcgcgcg gaggcgccgc cgctgcggcg ggagtagctg
     1981 aggagctgct tgacgaggtc ggcggcgcgc tgggtggcgt gtgtggcggt tttgattttc
     2041 tccttcacat cgccgccggg gctgatctcg gcgagggtga gattgccgcg gatggtggtg
     2101 agcatgttgt tgaagtcgtg ggcgatgccg ccggcgagct ggccgagggt gccgagtttt
     2161 tgcgactcgc gcagttgctg gcgcagaccg ctgagctcgg tgcggtcgcg caggagccag
     2221 aggcggccaa ggacttcgcc gctgtcgcca agcatggggg cggtgcgcag ctccatgatg
     2281 gcgccgccgt ccatcccggc ttccagagag cctttgaacg cgggttcttc aaggcactcg
     2341 cggatccagc cggcgacttt gccggagtcc tggaagcgcg cggcgaggag tccggcaaga
     2401 gtttcaaagt cgcgtccgcc gttttcatcg agcgggctgc cgaggaattc gtcgaggcgg
     2461 gcattggagg cgatgatggt gccgttgcca tccacgacgg cgacgccttc gtcgctggcg
     2521 gagaagatgc cctggagcgc ggtgaacgca tcgcgcagaa gacgctcggc attggcgagg
     2581 cgctgggcag tctggcagga gtgggtctcc gcagtgctga gcgcctggcg gtgcttggcg
     2641 aagagcatgt tgacgtcgca gcgcagcttc cagaagtcgg cgggcagcca ttcggccacg
     2701 tcttttgtcg gcgggatgcc gcgcatgagg gaggcggtgg cgttcatcat cgcggccacg
     2761 ggcttgagca cccagccgct caggagatag gcggcgagaa aaacaccccc gccgagcagc
     2821 accccctggg tgccgaaaaa ccagtcgagc gagtggccca tcagccgcgc ggcgcccatg
     2881 gccgggagga agccgaacaa aacaagcggc aggaggaagc ggagtcgtag cgagttcatg
     2941 agagggaggt ggcgaggatc gtcggagctt gcgcgaaagc agtgcggcgg tcggggaggg
     3001 ggaagccggg aggctggcgg aagaattccg ctgtgctcat gcgggtgatg agactttcct
     3061 ggcgggcgga gatcacttga tcgagagcgc atgcgatttt ttcgtccagg catgaggcga
     3121 gccggtcttc gatccatgcc aggatctgct cgcgagccgc ttccggcagg tcgttggcgg
     3181 gttgggaagg ggctggtgca tggtattcca gcctgtccag gcggtctttc agagcgctga
     3241 cacccatgac ggctgcgaag atgtcgttcc gaagctggtt gatgccctcg tcaatttccg
     3301 ttttccagga gtccactgcc ggtactgctg tcttgtcagc tccagggtct ggcagcagtt
     3361 cgccaggtgt ctttgctgcc gaggcaagac tcgcggcgat ggctgttcgg ggcatcactg
     3421 catgcgggat agattccggt ggctgcggca tgccccagct ttgattcact gccatggcgg
     3481 ccaagccgcc tgtgctcggt ggagaatcaa aaagaggaag cgccttttgg ggagtggagg
     3541 cattttgatt ctctttgtcg ggggacgcgg cggcttcatc caccgcccct gaaaaagagg
     3601 gaggttcgtc cgcaaaatgg cggcgctggg attggaacga gaaagcaagg ggcatgaact
     3661 gaatggcaat ataccgtgat tttatgacaa atatgaaaat tattccagca aaaaattaaa
     3721 ctttctttga taattcgagc ctggagagca aaatcggcag cctttgccgc atcagcgagt
     3781 ctctccgatc aagagaaggg cgtgatcttg actccgcctt cattccgccc taacccacgc
     3841 tggattttgc cacttttttg accatgcctc ccgcccacaa ctacactgaa gacagcatca
     3901 aatccctcga ctggcgcgag catattcgcc ttcggccagg aatgtatatt ggcaaactgg
     3961 gggacggttc ccagccggag gatggcattt atgtacttct caaagaggtt ctcgacaaca
     4021 gtattgatga acatgtcatg ggctacggaa aggtcatcga agtggagatc accgaggacc
     4081 atcaagtgac ggtgcgcgac tacggccgag gcatccctct gggcaagctc atggaatgcg
     4141 ccgctcagat taacacgggt gccaagtatg acagcgaggt cttcaagcgc tccgtgggcc
     4201 tcaacggcgt gggcatcaag gcggtgaacg ccctcagcac tttctttgag atacaggcca
     4261 tccgcgaaaa gcagacccgc gccattgagt tcagccaggg cattgtgaaa tacgacatgg
     4321 gcaggcccgc gccttgtgac gagccggatg gcacgcgcat taccttccgc ccggacacgg
     4381 agagtttcac cagccatgtg catttcaagc tggattatgt gcgcgagatg ctgcgctttt
     4441 actcctggct gaatcccggc ctcaccctca agctcaatgg tgagaccttt cgctccaaag
     4501 acggtctgcg cgacctgatc gccgccaagc tcaccgagga gccgctctac ccggtcgtct
     4561 atctcaccgg cacggacatc gaggttgcct tcacccacgg caccggttac ggcgaggagt
     4621 actacacctt cgtcaacggc cagcacacca cccagggcgg cacgcatctt gccgcttttc
     4681 gtgaagccat cgtgcaggaa tgccgtgctt acttcaacaa gcagtatgac cccaatgacg
     4741 ttcgcggttc cctgatcgcc gctgtttccg taaaagtgca ggagcctgtc tttgaaagcc
     4801 agaccaagac caagctaggc tccacccaca tggagcccaa cgggcgtaat ctgcgcaccc
     4861 acatcatcaa caccgtcgtc aaggaactcg acaactatct ccacaagcac ggtgacctcg
     4921 ccaaagcgct ccaggaaaaa atcaacgcca acgaaaagga gcgcaaagag atcagcggca
     4981 tccagaaagc cgcgcgcgac agcgcgcgca aggccaaggt ccacaacaaa aagctgcgcg
     5041 actgccgcat ccatctcgac accaaggaca agcgtcggga ggagagcacg ctcttcatca
     5101 ccgagggcga cagcgcctcc gggtccatca ccaaaagccg cgatgtcgag acccaggccg
     5161 tcttctcctt gcgcggcaag ccgctcaaca gctacggtct cagccgcaag atcgtctatg
     5221 agaacgagga gttctacctt ctggccaacg ccctccagat tgacgaaaac atcgaggacc
     5281 tgcgctacaa caagatcatc ctcgccaccg atgccgacgt ggacggaatg cacatccgcc
     5341 tgctgatgat caccttcttt ctccagttct tcccggagat cgttcgcgcc ggccacctgc
     5401 acatcctgca gacgcccctc ttccgcgtcc gcaacaagaa agagaccttc tactgctacg
     5461 gcgacgagga gcgccagcgt gccatccact gctgtggcaa gaacgcggag atcacccgtt
     5521 tcaaaggcct cggcgaaatt tcgcccgacg aattcaagca cttcataggc cccaaaatgc
     5581 gcctggaacc cgtcatgatg cccgagcaca agaccatcaa ggaactgctt gctttttaca
     5641 tgggtaaaaa cacaccggac cgccagacct atatcgttga taatctgcga gttgagcagg
     5701 agttgcgttt tacgggcgag gggggaggcg gggaaaa
//
LOCUS       DAOMYJ010000281         5730 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Prosthecobacter sp. isolate AMR_MDS_5629
            NODE_4794_length_5730_cov_18.929868, whole genome shotgun sequence.
ACCESSION   DAOMYJ010000281 DAOMYJ010000000
VERSION     DAOMYJ010000281.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867850
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prosthecobacter sp. (wastewater metagenome)
  ORGANISM  Prosthecobacter sp.
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Verrucomicrobiaceae; Prosthecobacter.
REFERENCE   1  (bases 1 to 5730)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5730)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.30
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:34:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,742
            CDSs (total)                      :: 4,684
            Genes (coding)                    :: 4,676
            CDSs (with protein)               :: 4,676
            Genes (RNA)                       :: 58
            tRNAs                             :: 55
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5730
                     /organism="Prosthecobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4794_length_5730_cov_18.929868"
                     /isolate="AMR_MDS_5629"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1965333"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..386)
                     /locus_tag="PLP58_19985"
     CDS             complement(<1..386)
                     /locus_tag="PLP58_19985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009962973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HRJ10872.1"
                     /translation="MPFKSLGLDPGILTAIQEAGYTEPTPIQSAAIPAVLAGGDVIGI
                     AQTGTGKTAAFTLPILTRLAQNPPQGQRRRTRVLVLAPTRELVAQIEENVLAYARHLP
                     LKIAKVYGGVGENPQKAALRSGADIV"
     gene            495..1694
                     /locus_tag="PLP58_19990"
     CDS             495..1694
                     /locus_tag="PLP58_19990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009960004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-like beta-propeller repeat protein"
                     /protein_id="HRJ10873.1"
                     /translation="MKAHPLLITTFLGGSTLFAADWAIWRGPTHDGISTESGWTLPAD
                     PQTLWEAEVGLGFSSFCVSEGRVVTTGHADDKDTVFCLDAKSGKEIWKHSYPADLGDK
                     YYEGGTSATPVFDGGKVYHLSRWGDLFCFEAVTGKVVWEKNLQKETGADLPDWGFAGA
                     PLVQGGLLILNVGKAGLAVEKDSGRIVWKSETDAAGYSTPYPITRGGKKLAVIGSAEA
                     YIAVEVETGKQVWSHRWNTRYGVNAADPILSGDLLFISSGYNKGCALLKLTDGAPEKV
                     WENKNLKNQFNSSVLLDGHLYGIDGDQNSRCALKCVRLSDGRELWDAKEAGFGSLMAA
                     DGKLIVLTSKGELIIAKATPAEFDVISRAQILNGKCWSAPVLANGLILARNSEGRVVC
                     VGVDRGK"
     gene            1748..2227
                     /locus_tag="PLP58_19995"
     CDS             1748..2227
                     /locus_tag="PLP58_19995"
                     /inference="COORDINATES: protein motif:HMM:NF013743.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein H"
                     /protein_id="HRJ10874.1"
                     /translation="MPLPTVRFKHARFSARFPESYRYSRSHYWMAPVEGQDGVWRVGF
                     TKFATRMLGELVDSEWKKQEGDDLAPGDIVGWVEGFKAASDVYSVMLGAFVEGNPHLK
                     QDACIVRSDPYEVGWLYAVRGEPEEDHLDVHGYIDHLSTIIQRMAEEGHGEEGAAEE"
     gene            complement(2256..2576)
                     /locus_tag="PLP58_20000"
     CDS             complement(2256..2576)
                     /locus_tag="PLP58_20000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ10875.1"
                     /translation="MSPESTAEKRYETYAGIIAPAAAGCALGILFGRGMSRGAANLVA
                     LGLLAGSAAVAAPLITDLVQRTANRPSSERGSRRRLESIRNSALPDEQLGDFFVDSPN
                     ALLP"
     gene            2659..3741
                     /locus_tag="PLP58_20005"
     CDS             2659..3741
                     /locus_tag="PLP58_20005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009958967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HRJ10876.1"
                     /translation="MDQPRKTSRYALILAGGSGQRFWPLSRDHLPKQLLKLFGSKTLL
                     EMTVDRLDGLVPRENILILTNTQQEAAVRALFPALPAENIIAEPEKRDTAPAIAIGIG
                     WVVARDPSATMIVLPADHLIKDDAGFRRVLDQAACAAEFGSSLVTVGIKPSWPCPSYG
                     YIERGQRVSLPGADAAPVYEVARFREKPNPELAEHFLAQGGFAWNAGMFIWSIPAIFS
                     ELTRHCPVLADFVSELRNSKDFMATVRGQFHRLPKLSIDYALMERATRVLNIEAGFDW
                     DDVGNWTSVGRYLDKDAAGNQHNAPLTTLDAADNLVFSQTGQHIALLGVHDLIVIAAG
                     DALLVTTRAQAENIKKLADKLPASLK"
     gene            complement(3795..4997)
                     /locus_tag="PLP58_20010"
     CDS             complement(3795..4997)
                     /locus_tag="PLP58_20010"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HRJ10877.1"
                     /translation="MKTALLFAALLLSMLHARADAVGEALEVFVKEGHIAGAVTLVAK
                     REQILSMQAVGWQVLETRKPMEQDSLFWIASMTKPVIALAILMLHDDGRLDIHDPVER
                     YLPEFKGQMLLQEKSDDRVVLVKPARPVTIKDLLTHTSGIAGNANAEPGPADSRTPRE
                     SAVICALTPLQFEPGSKWAYSNPGINTLGRIIEVVTGEDLSKAMSNYVFRPLNMKNTT
                     FLPTSKQIEKLAVSYKMDDELRRIVPSGIPYLSEPYTSTKRQPFGAGGLFSTAEDLHK
                     LCLMMVSGGEANRRKHLSKETLALMFQNHTGDMKAGFVEGMGMGLGLQVVARPTGVTA
                     KLSAGTAGHGGAHGTQMWFDPASGSIQIMLIQRAGLPNGDASPMRQAFHDAAAKVLQD
                     AASLPLEN"
     gene            5125..>5730
                     /locus_tag="PLP58_20015"
     CDS             5125..>5730
                     /locus_tag="PLP58_20015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009965066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HRJ10878.1"
                     /translation="MPASDALIQAARDLDAALKSLSFGPPTAYVYHPLDYARAPHEAY
                     LARYGGGKKRVVFVGMNPGPFGMTQTGVPFGEISAVRDWLDIREPVGRPEREHPKRRV
                     SGFDCKNSEVSGRRLWGLFKERFGTPEAFFKDHFVANYCPLVFMEEGGKNRTPDKLPA
                     HETGAMEALCDAHLRRVIEALEPEWVIGVGAFAEERAARAQA"
BASE COUNT          998 a         1833 c         1860 g         1039 t
ORIGIN      
        1 atgacgatgt ccgccccgga gcgcagggcg gctttttgag ggttctcgcc cacgccgcca
       61 tagactttgg cgattttcaa tggcaggtgc ctcgcataag cgaggacgtt ttcctcgatc
      121 tgcgcgacca gctcgcgcgt gggcgccagc acgagcacgc gggtgcggcg acgctggcct
      181 tggggcgggt tttgcgccag gcgcgtgagg atgggcaggg tgaaagcggc ggtcttgccc
      241 gtgcctgtct gggcgatgcc gatgacatcc ccccctgcga gcacggcggg gatggcggcg
      301 gattgaatgg gagtgggctc ggtgtagccg gcctcctgga tggcggtgag gatgccgggg
      361 tcaaggccga gggatttgaa aggcatgcgc gcccataagc gcggcgcgcg cgggatgcga
      421 gccggatttg cacagcgcgt actgccagcc ttcgcggttg ccagaagcgg cgggccgggc
      481 gttaatcccg ccgcatgaaa gctcacccac ttctcatcac aacatttctc ggaggttcca
      541 ccctcttcgc ggcggactgg gccatctggc gcggacccac ccatgacggc atctccaccg
      601 agtcaggctg gacgctgcca gcggatccgc agaccctttg ggaggccgaa gtgggccttg
      661 ggttctcctc cttctgcgtg tccgaaggca gggtcgtcac caccggccac gccgatgaca
      721 aggacacggt cttctgcctc gacgcgaaga gtggcaagga aatctggaag cacagctacc
      781 ccgccgatct cggagacaag tattatgaag gaggcacctc ggccacaccg gtcttcgacg
      841 gcggcaaggt ttatcatttg agccgctggg gtgatctttt ctgcttcgag gccgtcacgg
      901 gcaaggttgt ctgggagaag aacttgcaga aggagaccgg cgcggacttg ccggactggg
      961 gcttcgccgg cgcgccgctc gtccagggcg ggctgctcat tctgaatgtc ggcaaggccg
     1021 gcctcgccgt cgaaaaggac agcggcagga tcgtgtggaa aagcgagacc gacgccgccg
     1081 gatactccac gccttatccc atcacccggg gcggcaaaaa gctggccgtc atcggctccg
     1141 cggaggctta catcgctgtc gaggttgaga caggaaaaca ggtctggtcc caccgctgga
     1201 acacccgcta tggtgtgaat gccgccgacc ccatcctcag tggcgacctg cttttcatct
     1261 ccagcggcta caacaaaggc tgcgctcttc tgaaactcac cgacggcgcg ccggagaaag
     1321 tctgggaaaa caaaaacctc aaaaaccagt tcaactccag cgtcctgctc gatggccatc
     1381 tctacggcat tgatggcgat caaaacagcc gctgcgccct caaatgtgtg cgcctcagcg
     1441 atggcaggga gttgtgggac gccaaggaag ccggcttcgg ctccctcatg gccgccgatg
     1501 gcaagctgat cgtcctgacc tccaaaggcg agctcatcat cgccaaggcc acgcctgccg
     1561 agttcgatgt catcagccgc gcgcaaatcc tcaatggcaa atgctggagc gctcccgtcc
     1621 tggccaacgg actcatcctc gcccgcaact cggaaggccg cgttgtttgt gtcggggtgg
     1681 atcgcggcaa gtgaggtttg atgatggatg aatgcggagt gacgatagcc gacagcccac
     1741 agtccgaatg cctctcccca ccgtccgctt caagcacgcc cgcttctcgg cccgctttcc
     1801 cgagtcgtac cgctactcgc gcagtcatta ctggatggct cccgtggaag gtcaggatgg
     1861 agtgtggcgg gtgggcttta ccaaattcgc cacacgcatg ctgggggagc tggtggacag
     1921 cgagtggaag aagcaggaag gcgatgacct ggctccaggc gacatcgtcg gctgggtgga
     1981 gggcttcaag gcggcgtcag acgtgtattc ggtgatgttg ggagcgtttg tcgaaggcaa
     2041 tccccacctc aagcaggacg cctgcatcgt ccgcagtgat ccttatgagg tcggctggct
     2101 ttatgccgtt cgtggagaac ccgaggagga ccacctcgac gttcacggct acatagacca
     2161 cctgagcacc atcatccaac gcatggccga ggaaggccac ggggaggagg gcgcggctga
     2221 ggaatgatca cctgccattc cagttcgcgc gctgttcagg gcagaagcgc gttgggcgag
     2281 tccacaaaga agtcgccgag ttgctcatcg ggcagggcgc tgttgcggat gctttccagg
     2341 cgcctgcggc tgccgcgttc ggaggagggc cggttggccg tgcgctgcac caggtcggtg
     2401 atgagcggcg cggccaccgc cgcgctgcca gccagcaggc ccagcgccac gagattggcc
     2461 gcgccgcggc tcatgccgcg cccgaagagg atgcccagcg cgcagccagc ggccgccggg
     2521 gcgatgatcc cagcgtaggt ttcgtagcgt ttttcggcgg tggactcggg tgacatggag
     2581 gtttccatac agcatcgcgg cactttgtcc aaagtggaat ttgcccggca cggtgtttgc
     2641 tccatgatgg gcgcttccat ggaccagccc cgcaagacct cccgctacgc gctcatcctc
     2701 gccggaggca gcggccagcg cttctggccg ctcagccgcg atcatctgcc caagcagttg
     2761 ctcaagctct tcggctcgaa aaccctgctg gagatgaccg tggaccggct tgatggcctc
     2821 gtgccccgcg agaacatcct cattctcacc aacacccagc aggaggcggc cgtacgggcg
     2881 cttttccccg cgctgccggc ggagaacatc atcgccgagc cggagaagcg ggacaccgcg
     2941 ccggccatcg caattggcat cggctgggtg gtcgcgcgcg acccgtccgc caccatgatc
     3001 gtgctgccgg ccgatcattt gatcaaggac gacgccggct tccgccgcgt gctcgaccag
     3061 gccgcctgcg ccgccgagtt tggcagcagc ctggtcaccg ttggcatcaa gccctcatgg
     3121 ccctgcccca gttacgggta catcgagcgc ggccagcgtg tctcgctgcc cggcgcggac
     3181 gccgcgcccg tttacgaggt ggcgcgcttc cgtgaaaagc ccaaccccga gctggccgag
     3241 cactttctcg cgcagggagg cttcgcctgg aacgcgggca tgttcatctg gagcatcccc
     3301 gccattttca gcgagctgac gcggcactgc cccgtgctgg ccgacttcgt ctccgagctg
     3361 cgcaattcga aggacttcat ggccactgtg cgcggccagt ttcaccggct tcccaaactc
     3421 tcgattgatt acgcgcttat ggagcgcgcc acgcgcgtgc tgaacatcga agccggtttc
     3481 gactgggatg acgtgggcaa ctggacctcc gtgggccgtt atctcgacaa ggacgccgcc
     3541 ggcaaccagc acaacgcgcc cctcaccacg ctagatgccg ctgacaacct cgtcttttcg
     3601 cagaccggcc agcacatcgc cctgctgggc gtgcatgact tgattgtcat cgccgccggg
     3661 gacgccctgc ttgtcaccac gcgcgcccag gctgagaaca tcaaaaaact ggccgacaag
     3721 ctgcccgcca gcctcaagtg aagccggggt ggccgctgac ttgggaagac ggcacggagg
     3781 ctcccggccc ggaatcaatt ctccaagggc agactggccg cgtcttgcag caccttcgcc
     3841 gccgcgtcat gaaaggcctg gcgcatgggc gaggcatccc cgttcggcag gccggcgcgc
     3901 tggatgagca tgatctggat ggagccggag gcggggtcga accacatttg cgtgccgtgc
     3961 gcgccgccat gccccgcagt gcccgcgctg agcttggcgg tgacgccggt gggccgggcc
     4021 accacctgga ggccgaggcc catgcccatg ccttcgacga agccggcctt catgtctccc
     4081 gtgtggtttt ggaacatcag cgccagggtt tccttcgaaa gatgcttgcg ccggttggcc
     4141 tcgccgccgc tgaccatcat cagacagagc ttgtgcagat cctccgccgt ggagaacagc
     4201 ccgcccgcgc cgaagggctg gcgcttggtg gaggtgtaag gctcgctcaa atatggaatg
     4261 ccggagggca cgatgcggcg cagctcgtca tccatcttgt aggacaccgc cagtttctcg
     4321 atctgcttgg aggtgggcag aaaggtggtg tttttcatgt tcagggggcg gaaaacgtag
     4381 ttcgacatgg ctttggaaag gtcctccccg gtgacgacct cgatgatgcg gcccagggtg
     4441 ttgatgcccg gattcgagta agcccatttg ctgccgggtt cgaactgcaa gggggtcagc
     4501 gcgcagatga ccgcggattc gcgcggcgtg cgggagtccg cggggcctgg ctcggcgttc
     4561 gcattgcccg cgatgccgct ggtgtgggtg agaaggtctt tgatcgtgac cggacgcgcg
     4621 ggcttcacca gcaccacgcg gtcatcgctt ttttcctgga gcagcatctg ccctttgaac
     4681 tcgggcagat acctctccac cgggtcatgg atgtccaggc gcccgtcgtc atgcagcatc
     4741 aggatcgcaa gggcgatgac gggcttggtc atcgaggcga tccagaacag gctgtcctgc
     4801 tccatcggtt tccgcgtctc cagcacctgc cagcccaccg cttgcatgct gagaatctgc
     4861 tcgcgtttcg ccaccagggt gaccgctccg gcgatgtgcc cctctttgac aaaaacctcc
     4921 agcgcctcgc cgacagcgtc agcgcgggca tggagcatgg acaggagcag ggcggcgaaa
     4981 agaagggcgg ttttcatgaa ggtggatcgg atcttcatcg tggatgcttg cgcgcggaat
     5041 gcaaaatttc agccttccat tctccccccc ttgaatggtg acgcccgctt gacccgccgc
     5101 gcctgcggct ccacctcgtc cgccatgccc gcctcagacg ccctcatcca ggccgcgcgt
     5161 gatctcgacg ccgcgctcaa gtccctctcc ttcggcccgc cgaccgccta tgtgtatcat
     5221 ccactggact acgcccgcgc cccgcacgag gcctacctcg cgcgctatgg cggggggaaa
     5281 aagcgcgtgg tttttgtcgg catgaatcca gggcctttcg gcatgacgca gacgggggtg
     5341 cccttcggcg agatttccgc cgtgcgtgac tggctcgaca tccgcgagcc ggtgggtcgc
     5401 ccggagcgcg agcatccgaa gcgccgcgtg tccggttttg actgcaagaa ctccgaggtc
     5461 agcggacgcc gcctctgggg cctgttcaag gagcgcttcg gcacgccgga ggcgtttttc
     5521 aaagatcact tcgtggccaa ctactgcccg ctcgtcttca tggaggaggg tggcaaaaac
     5581 cgcacgccgg acaagctgcc cgcccacgag accggggcca tggaggcctt gtgcgacgcg
     5641 catctgcgcc gtgtgatcga ggcgcttgag ccggagtggg tcatcggcgt cggcgccttc
     5701 gccgaggagc gcgccgcgcg cgcccaggcc
//
LOCUS       DAOMYJ010000282         5727 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Prosthecobacter sp. isolate AMR_MDS_5629
            NODE_4803_length_5727_cov_23.140691, whole genome shotgun sequence.
ACCESSION   DAOMYJ010000282 DAOMYJ010000000
VERSION     DAOMYJ010000282.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867850
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prosthecobacter sp. (wastewater metagenome)
  ORGANISM  Prosthecobacter sp.
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Verrucomicrobiaceae; Prosthecobacter.
REFERENCE   1  (bases 1 to 5727)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5727)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.30
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:34:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,742
            CDSs (total)                      :: 4,684
            Genes (coding)                    :: 4,676
            CDSs (with protein)               :: 4,676
            Genes (RNA)                       :: 58
            tRNAs                             :: 55
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5727
                     /organism="Prosthecobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4803_length_5727_cov_23.140691"
                     /isolate="AMR_MDS_5629"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1965333"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            101..3064
                     /locus_tag="PLP58_20020"
     CDS             101..3064
                     /locus_tag="PLP58_20020"
                     /inference="COORDINATES: protein motif:HMM:NF040138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HRJ10879.1"
                     /translation="MNIGFAGTVYALVLDSTDTLYAGGTFGWANAANSGDMGATVVNG
                     VAKWNGSAWEALGTGADGADGANDLNALAVDSSDNVYAGGNFATMSGVANTARIAKWN
                     GSAWSALGRGVSNEVNALLVFGNELYVAGSFTYADAPTSSTPGALTANRIARFNLNDN
                     TWGLLGSGMNTGAIINALAVTGTDLYVGGSFVTAGGKVAGYAAYADINPPPVVNAGAN
                     GTVDEGSAFVQAGSFDDPGMDSWTATVNYGDGSGVQALTLDQMAKTFELNHTYADNGV
                     YTITVVVTDSDLRDGSDTVQVTVSNVAPDFELGLPDPEFLAVGSGDFSRTVDFTDPGD
                     DVWSGTANYGAGGGNVALSVDQMAKTFDLTATYTLGGLFTVSATLQDDDLGSHNDSMT
                     VDVLLEPIPVTGAASGITAYSATLNGTVNPNHSQLRGATTAWFEYSRDPAMMEGIMQS
                     SSVPVASTGNNPAAVPVSIEIADLLADEDYYFRMVAENEAGTTYGDIQSLHSRYPFAT
                     GDFMLADEGLLGGGELSRPIEGVINNAGRVSFKAAGKVGSGGITSSNDSLLLSDASGE
                     LKVIARESTTVEYQESPYIWSTLNGTFDDPILSETGETVFRDKILGTTTLKDQGYFIS
                     PDGEALEILSREGDAAPDAGTFTLHTGKPVMDSDGRVYFQGKVSGLTTKTDTGIWFED
                     IGTPLERLVAEGQDVSDALPSGIGWLGQLSGDIAAGGDGVGFIAALQHNPANSDEKTD
                     TALNQAAFSCSSAGDLEVILRKGDLVDGAGVSKLTGIARGSSGDHAVLATLAAPATTA
                     DDLVLVYKGLSGVELVAREGVTVISGAATLASVSEFHVTTDGAVVFLGKVKGDGTTTV
                     TDGVVCKWTSGGGVTLLAREGSTANGTGSNYGAIQALSVSPGGVVLVQATLTNGRVAA
                     LSDTGGTLDLLVQTTDTVTYNGSPLSVISLSIYKGTTGIGGGGGGMGAAINDSGDALI
                     MISLGGGVHIARIYR"
     gene            complement(3362..4009)
                     /locus_tag="PLP58_20025"
     CDS             complement(3362..4009)
                     /locus_tag="PLP58_20025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ10880.1"
                     /translation="MPRLLIPAAAALLGLCSCQSLKKDQVVITVHAQGTDMDSKKTVF
                     TRPIGGRTVIFKIIPEFSTQSMAAFHPFPADNGTQGVAMKLDFKGTNSLELVTRLRHG
                     EMLMTMVNGQVVDHVIIDRPITDGIFTIWQGFSDEQIAALDEEYPRIKDLKSSSTFIE
                     MTPSTATEKKQSKRRAEDEEKQRKADEKRRARGGSDPEAPDGELVPLSELLKTSR"
     gene            4049..>5727
                     /locus_tag="PLP58_20030"
     CDS             4049..>5727
                     /locus_tag="PLP58_20030"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HRJ10881.1"
                     /translation="MEKEIVRSARAGMMRGHYHARPVTVPPPDSPTLITGKGPPARDT
                     GPLTAGEKDSALPRAGAPRTETGEWLDGKFRLMEKLGEGGFGLVYKAEQTQPIHRFVA
                     VKVLKAGMDTRQVIARFETERQSLALMEHPNIARVLDAGETAAGQPYFVMELVRGMSI
                     TKHTREKHLPLRARLELFIPVCHAVNHAHQKGIIHRDLKPSNIMVMEEDGAPVPKVID
                     FGIAKVLEQKDASQTLATGMDQLVGTPGYISPEQIQHGSSHVDTRSDVYALGSILLEL
                     LTGKGLVTPMDLAHKPIHLILRDQVDLDPPRPSSRDPDLKGDLDWITLKALEREPARR
                     YGSADDLADDLRRFLDHQPVRARPPSRRYLLEKFVRRHRVGVAASAAIALAVLGGGVT
                     STALYLQAERNQRLLIKSYSRSDEQMARQLGERADHSEAVARLCRALRTDPENGPAAV
                     NLLSLLQHVHLLHPLTPELPLPEGMTSARLTVLSPQTRRLLALGSVSDDGGKNDTLCV
                     WDTVSQARRDFPLPSGVLATGLLATPDGSHAILTRDDGQVELLTLPSAGRAP"
BASE COUNT         1069 a         1816 c         1789 g         1053 t
ORIGIN      
        1 ggggaatttg tatgtcggag gggcattcac cacggtcagc ttaggcccga cgataccggc
       61 ctcgcgcgtg gccagttgga acggcacttc atggagctca atgaacatcg gatttgccgg
      121 aaccgtttat gcgctggtgc tggacagtac cgacaccctg tatgcgggcg ggacatttgg
      181 atgggcgaat gccgccaact caggcgacat gggagccact gtggtgaacg gagtcgccaa
      241 gtggaacggc tccgcctggg aggctctggg cacaggggcg gacggggcgg acggggccaa
      301 tgatctgaac gctcttgcgg ttgattcctc ggacaatgtt tacgcaggcg ggaatttcgc
      361 gaccatgagc ggtgtggcga acacggcgag gatcgccaaa tggaacggct ccgcatggtc
      421 ggcgctgggg cgcggtgtca gcaatgaagt gaatgcgctg ctggttttcg gaaacgagct
      481 gtatgtggcg gggtcgttta cttatgcgga tgcgccgaca agctcaacgc ccggcgctct
      541 gacagcgaac cgaatcgctc gtttcaacct caacgacaac acctggggcc tgcttggctc
      601 cggcatgaat actggcgcga tcatcaacgc gctggcggtg actggaaccg atctgtatgt
      661 ggggggcagc tttgtgaccg ctggcggcaa ggtggccggt tacgcggcct acgctgacat
      721 caacccgccg cctgtcgtga atgcgggcgc gaacggtacg gtggacgagg gcagcgcttt
      781 tgtacaggcc ggctcgttcg atgatccggg catggactcg tggaccgcca ctgttaacta
      841 tggcgatggc agcggggttc aggccctgac tctggaccag atggccaaga ccttcgaact
      901 gaaccacacc tatgctgaca acggcgtcta caccatcact gtggtcgtca ccgacagtga
      961 tttgcgggat ggctcggaca cggtgcaggt cacagtgagc aatgtggcgc ctgattttga
     1021 gcttggactc cctgatccag agttcctggc cgttggctca ggcgatttct cgcggacggt
     1081 ggattttacc gaccctggag atgatgtctg gagcggcact gccaactatg gagccggagg
     1141 cggcaatgtc gcgctgtctg tggaccagat ggccaagaca ttcgatttga ccgcgactta
     1201 cacccttggc ggtcttttca ccgtcagcgc caccctgcag gatgatgact tgggcagcca
     1261 caacgactcc atgacagtgg atgttcttct tgaacccatc ccggtcactg gcgcggcatc
     1321 cggcatcacc gcttactcgg cgaccttgaa cggaaccgtc aaccccaacc acagccaact
     1381 ccgcggcgcg acgacagcat ggtttgaata ctccagagat cctgcgatga tggaaggaat
     1441 catgcaaagc tcgtcagtgc cggtagccag caccggcaac aaccccgccg ctgtgccagt
     1501 gagcatcgag atcgccgacc tgctcgcaga tgaggattac tatttccgaa tggtggcgga
     1561 aaacgaggcg ggaaccacct atggcgacat tcagagcctg cattcacgct atcctttcgc
     1621 caccggagac ttcatgctgg ccgatgaggg attgctaggc ggcggggaac tttccaggcc
     1681 catcgaaggg gtgatcaaca atgccgggcg cgtgtctttc aaagcggcag gcaaagtcgg
     1741 cagcggtggc atcacctcaa gcaatgacag cctgctgctc agcgacgcca gcggggaatt
     1801 gaaagtgatc gcgcgtgaaa gcacgaccgt ggagtaccag gaatcgccat acatctggag
     1861 cacgctcaac ggcacgtttg acgatcctat tttgagcgag acaggggaga ccgtcttccg
     1921 ggacaaaatc ctgggcacca caactttgaa agaccagggc tatttcattt caccggatgg
     1981 agaagccctg gaaatcctga gtcgcgaggg agacgccgcg cccgatgcgg gcacattcac
     2041 tttgcacaca ggaaaacccg tcatggattc tgatggcagg gtttacttcc aaggcaaggt
     2101 ttctgggctg accaccaaaa ccgataccgg catctggttt gaggacattg gcacccccct
     2161 ggagcgcctg gtcgcggaag gtcaggatgt ttctgacgcg ctgccctccg gcattggctg
     2221 gctcggccag ttgtccggcg acattgccgc cggcggtgac ggagtgggtt ttatcgcggc
     2281 cctgcagcac aatccggcga actcggatga aaaaactgac accgcactca atcaagccgc
     2341 cttctcctgc tccagcgcag gagaccttga agtgatcctt cgaaagggag atttggtgga
     2401 tggcgcgggc gtctccaaat tgacaggcat cgcgcgcggc agcagcggcg atcacgcggt
     2461 ccttgccaca ctcgcagctc cagcgaccac agcggatgac ctggtactgg tttacaaggg
     2521 tttgtcgggc gttgaactcg tggctcgcga gggtgtcaca gtcatttccg gggctgcgac
     2581 ccttgccagt gtcagcgaat ttcatgtgac caccgacggc gcggtggttt tccttggcaa
     2641 agtgaagggt gatggaacca ccacggtcac ggatggcgtc gtctgcaaat ggacttcggg
     2701 aggcggcgtc actcttctcg cccgcgaagg ttccaccgcc aatggaaccg ggtccaatta
     2761 cggagccatc caggcgctct ctgtcagtcc cgggggagtg gttttggttc aagcgacgct
     2821 gaccaacggc agggttgccg ccttgagcga cacgggaggc accttggact tgctggtgca
     2881 gaccactgac accgtgacct acaatggctc ccctctctcc gtcatctcct tgtccatcta
     2941 caaaggcacc acgggcatcg gcggcggcgg cggcggcatg ggtgcggcca tcaatgactc
     3001 cggggacgct ttgatcatga tctccctcgg tggcggtgtg catatcgccc gaatctatcg
     3061 ttgattgcgt ggcacctact gcaccggcgt attcagtgtc ttgaccctac acaaaccggc
     3121 ccatcatccc gctccagggg tggtgggctg tgttgtttga ggcagggaag agtcggcgtg
     3181 ggttgccgtg aatcctggat tgctccgcac cattgccacc attgacagaa tgctgctttt
     3241 tgtttcccgg caccgccggg aatcagcccg ctgtgcgcat gaaacgcgaa atgcttggca
     3301 tccgggttca aaacaccctt tgtggaatga atccttccga atagacaaag gtgccaggcg
     3361 ctcagcggga ggtcttgagc agttcggaga gggggaccag ctcgccatcg ggggcctcgg
     3421 ggtcggagcc gccgcgggcg cggcgtttct catcggcttt gcgctgcttt tcctcatcct
     3481 cggcgcggcg cttggactgc tttttctccg tggcggtgga aggggtcatc tcgatgaagg
     3541 tggaggagga tttgaggtcc ttgatgcggg gatactcctc gtcgagggcg gcgatctgct
     3601 catcgctaaa accctgccag atggtgaaga tgccgtcggt gatggggcgg tctatgatga
     3661 catggtccac cacctggccg ttgaccatgg tcatgagcat ctcgccatga cgcaggcggg
     3721 tgacgagttc gagggagttg gtgcccttga agtcgagctt catggccacg ccctgggttc
     3781 cgttgtcggc gggaaagggg tggaaggcgg ccatgctctg cgtgctgaac tcggggatga
     3841 tcttgaagat gaccgtgcgg ccgccgatgg ggcgggtgaa gacggtcttc ttcgagtcca
     3901 tgtccgtgcc ctgcgcgtgg acggtgatga ccacctggtc ttttttcagg ctctggcagg
     3961 agcagagacc gaggagcgcg gcggcggcgg ggatgagcag gcgtggcatg atgtcagtga
     4021 tggcgcggcg cgggcttttg gcaagagggt ggagaaggag attgtcagaa gcgcgcgcgc
     4081 gggcatgatg cgcggccact accacgcgcg ccctgtgact gtcccgcctc cagactcccc
     4141 cacactgatc accggcaaag gcccgccggc tcgggacacg ggccccctga ctgcgggcga
     4201 aaaggattcc gccctgccca gggctggagc gcccaggacg gagaccgggg agtggctgga
     4261 cggcaagttc cgcctcatgg agaagctggg ggaaggcggg ttcgggctgg tgtacaaagc
     4321 ggagcagacc cagcctatcc accgcttcgt ggccgttaag gtgctcaagg ccggcatgga
     4381 cacgcgccag gtcatcgccc gcttcgagac cgagaggcag agcttggcgc tcatggagca
     4441 cccaaacatc gcccgcgtgc tcgacgccgg cgagaccgcc gcaggccagc cctacttcgt
     4501 catggagctg gtgcgcggca tgtccatcac gaaacacacg cgggagaagc acctccccct
     4561 gcgcgcgcgg ctggagcttt tcatccccgt ctgccacgcc gtgaaccacg cgcatcagaa
     4621 gggcatcatc caccgcgact tgaagccctc caacatcatg gttatggagg aggacggcgc
     4681 gcccgtgccc aaggtcattg acttcggcat cgccaaggtg ctggagcaaa aggatgccag
     4741 ccagacactc gccaccggca tggaccagct cgtcggcacc cccggctaca tcagcccgga
     4801 gcagatccag cacggcagct cccatgtgga cacgcgctcg gacgtgtacg ctctcggctc
     4861 catcctcctc gaactgctca ccggcaaggg cctggtcacc cccatggacc tcgcccacaa
     4921 gcccatccac ctcatcctgc gcgaccaggt ggacctcgac ccgccgcgcc ccagctcccg
     4981 cgatcccgac ctcaagggcg acctcgactg gatcaccctc aaagccctgg agcgcgagcc
     5041 cgcccgccgt tacggcagcg ccgacgacct cgccgacgac ctccggcgtt tcctcgacca
     5101 ccagcccgtg cgcgcccgcc ctcccagccg ccgctacctg ctggagaaat tcgtccgccg
     5161 ccaccgcgtc ggtgtcgccg cctccgccgc catcgccctc gccgtcctcg gcggcggcgt
     5221 caccagcacc gccctctacc tccaggccga gcgcaaccag cgccttctca tcaagtccta
     5281 ctcccgctcc gacgagcaaa tggcccgcca gctcggcgag cgcgccgacc actccgaggc
     5341 cgtcgcccgc ctttgccgcg ccctgcgcac cgacccggaa aacggccccg ccgccgtcaa
     5401 cctcctctcc ctcctccagc acgtccacct tctccacccc ctcacgcccg agctgcccct
     5461 gcccgagggc atgaccagcg cccgcctcac cgtcctcagc ccccagaccc gccgcctgct
     5521 cgcccttggc tccgtcagcg atgatggggg gaaaaatgac accctctgcg tctgggacac
     5581 cgtgtcacag gcccggaggg actttcccct gccctccggc gtgctcgcca ccggcctgct
     5641 cgccaccccg gacggctccc acgccatcct gactcgcgat gacggccagg tcgagctgct
     5701 caccctgcca agcgccggcc gcgctcc
//
LOCUS       DAOMYJ010000283         5720 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Prosthecobacter sp. isolate AMR_MDS_5629
            NODE_4813_length_5720_cov_17.887026, whole genome shotgun sequence.
ACCESSION   DAOMYJ010000283 DAOMYJ010000000
VERSION     DAOMYJ010000283.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867850
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prosthecobacter sp. (wastewater metagenome)
  ORGANISM  Prosthecobacter sp.
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Verrucomicrobiaceae; Prosthecobacter.
REFERENCE   1  (bases 1 to 5720)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5720)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.30
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:34:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,742
            CDSs (total)                      :: 4,684
            Genes (coding)                    :: 4,676
            CDSs (with protein)               :: 4,676
            Genes (RNA)                       :: 58
            tRNAs                             :: 55
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5720
                     /organism="Prosthecobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4813_length_5720_cov_17.887026"
                     /isolate="AMR_MDS_5629"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1965333"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1072)
                     /locus_tag="PLP58_20035"
     CDS             complement(<1..1072)
                     /locus_tag="PLP58_20035"
                     /EC_number="2.7.14.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009959293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0046314 - phosphocreatine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein arginine kinase"
                     /protein_id="HRJ10882.1"
                     /translation="MRFNTLINHPADWMKGSGPHSEIVMTSRVRLARNLRGHSFPGYS
                     PEARRVALLQEALPHVTSLPRMDGGYSDEYSALSKVRKQVLVERHLVSREHAARSAGS
                     AVVLDRAQSLSIMINEEDHFRIQGIRPGLALRSAWQLVDEADSALEALLPYAFDERLG
                     YLTACPTNLGTGMRASVMLHLPALVLTEQINPALKAVGKIGLAVRGLYGEGTEALGNL
                     FQISNQHTLGESESEIITEIEKVIHGVVRAETNARAKLLQDHTLMLHDQVGRAYAILR
                     HAHILTSKDALNLLSLLRLGADLDIIPACDRGLIDMLLLGIQPAHLQFGAGRDLSPEE
                     RDALRAQITRAHLQAVPPPQPPA"
     gene            complement(1105..1650)
                     /locus_tag="PLP58_20040"
     CDS             complement(1105..1650)
                     /locus_tag="PLP58_20040"
                     /inference="COORDINATES: protein motif:HMM:NF014232.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrB/UvrC motif-containing protein"
                     /protein_id="HRJ10883.1"
                     /translation="MNCDVCEAEATVFLTQIINGQMTTVNLCDACSKAKGVTDETGFG
                     LAEAFLTPPHPHSEIETACPACGFTSAQLKKIGRMGCPECYTTFRQHIEGLLKSMHKG
                     TQHTGKKPRHLHIAAPQAAPVAFPESSAPAPPPPAKARKKASLPELRAQLEQAVRDER
                     YEDAARIKARMDAIESGQDPA"
     gene            1844..4081
                     /locus_tag="PLP58_20045"
     CDS             1844..4081
                     /locus_tag="PLP58_20045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ10884.1"
                     /translation="MMDPPDETPRKPRVRLTPWRGPFHFLLLLATSSVTLLLPLLYAL
                     LLLALGFHVGALAFGWIEHLALAETRAWRELLVIPYIAVGAVTWVFMLRPLLPRPRST
                     RVAMQVTPTSQPALFELVDELCWNLRLEPPEEIWLDTTTSVRTSLRNGVSGALTGESV
                     IHVGLPVVSVLGAREFAALVARELAMNTGGLGTTFSHLVRELNTWFYRALHERDPWEL
                     RLRQKQEKETRWQAGCRVAIWLWMAAAKLPFAFFVLAARVISAAALMRLGSGADKAGV
                     NLAGRAAWRAMQAKLRLLDAAWESARVEVRRGISQHRLPDNLSLLIARHVARAAGDGC
                     PAEPAGGPEPKGARIISGLEENAPAAVLLRGFVDLSRQLTSFYYQHDLGIQIHEHRLV
                     AEEEVIHQNRREDESLVIIRRYFSGLAHPERAMCGHAPTHAFSPGRERLENEIRRVRQ
                     DIREWGSRLKPALQEWNLAWQRRRDLEAAAVLSLGGFNVSRIQFGTEDTSPQSLRQEA
                     ARQRMLMEHMETALTAFEAKMESRFAAALGLLWWSAPDELDARLIERRQDLPHWVGLY
                     EAMAGALPSFRELLTTFFAFQTLGARFSSLDDAGPMFQALQSVVPKMINLTRQILSAM
                     DGAQYAFTSNRRPMPLVAHMLPGRLPEPVGISMDPAAAADTKALSAKMAADASEVVAP
                     FVDRFMQLWHKSYAWLAESAELTEMHFLGPMSLSAGTAELLTPEDMARPAPGAGAAAA
                     KTKAA"
     gene            4142..5125
                     /locus_tag="PLP58_20050"
     CDS             4142..5125
                     /locus_tag="PLP58_20050"
                     /inference="COORDINATES: protein motif:HMM:NF024989.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-enoyl thioester reductase domain-containing
                     protein"
                     /protein_id="HRJ10885.1"
                     /translation="MSRCLSFAATGNPAEVLELIERPLPALGPHDVRVHMRYSPVNPA
                     DLNFIEGTYGRPAHPPAIPGHEGCGEVVETGAEVASLKNGDVVIPLLGAGCWTRHMTA
                     AEHFFAKLPPGVDRVQASMLRINPVTAWHLLHHFIPLEDGAWVAQNAGNSGVGRCVIQ
                     IARQLGLKTASFARRPELVEELRALGADAVYLDDEAGLAAAKKDLHGSEIRLAANAVS
                     GDSAIRLMDLLSPGGTLVTYGAMSRRSLKVPNKFLIFKDLVLRGLWITRWLEHAGTDE
                     LFKALDPLAKMILHGELVQPVDEIIPMSEHRRAMRRAQETGRTGKIILDLA"
     gene            5122..>5720
                     /locus_tag="PLP58_20055"
     CDS             5122..>5720
                     /locus_tag="PLP58_20055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ10886.1"
                     /translation="MSADTHAIFKVMRPFLLIPLLLSTTAPLRAAPEIISPQVKASVD
                     KAVAWLLRQQHPSGYFSAEKTDKQPQRGDIPSHSAAMTALTLMGLASVGHLPGNPGSE
                     GEAAARALKFIVDHVEPDENGYLGRTDRSRMYGHGIITLMLTEMLGMAADEETDRKAR
                     AMTAKAIQLIIRAQQTPKSEANRGGWRYEPSSSDSDISVS"
BASE COUNT          884 a         1776 c         2118 g          942 t
ORIGIN      
        1 cggcgggggg ctggggcggc gggacggcct ggaggtgggc gcgggtgatc tgggcgcgca
       61 gggcgtcgcg ctcctcaggg ctgagatcgc gcccggcgcc gaattggagg tgggcaggct
      121 ggatgccgag caggagcatg tctatgaggc cgcggtcgca ggcggggatg atgtcgaggt
      181 cggcgccgag gcggaggagg gagaggaggt tgagcgcgtc cttggaggtg aggatgtggg
      241 cgtggcgcag gatggcgtag gcgcggccga cctggtcgtg gagcatgagg gtgtggtcct
      301 ggaggagctt ggcgcgggcg ttggtctcgg ctcggacgac gccgtggatg actttttcga
      361 tctcggtgat gatctcggac tcgctctctc caagggtgtg ctggttggag atttggaaaa
      421 ggttgccgag ggcctcggtg ccctcgccat agaggccgcg gacggcgagg ccgatcttgc
      481 cgacggcttt gagggcgggg ttgatctgct cggtaaggac gagggcgggg aggtggagca
      541 tgacggaggc tctcatgccg gtgcctaggt tggtggggca ggcggtgagg tagccgaggc
      601 gctcgtcaaa ggcgtagggg aggagggctt cgagggcgct gtcggcctcg tccacgagct
      661 gccaggcgct gcgcagcgcg agtccggggc ggatgccctg gatgcggaag tggtcctcct
      721 cgttgatcat gatggagagg ctctgggcgc ggtcgagcac gacggcgctg ccggcggagc
      781 gggcggcgtg ctcgcggctg acgaggtggc gctcgacgag gacctgcttg cgcactttgc
      841 tcaaggcgga gtattcgtcg ctgtagccgc cgtccatgcg cggcaggctg gtgacgtgag
      901 gcagggcctc ctggaggagg gccacgcgcc gggcctcggg ggagtagccg gggaagctgt
      961 ggccgcgcag attccgggcg aggcggacgc gggaggtcat gacgatctcg gaatgggggc
     1021 cgctgccttt catccagtcg gcagggtggt tgatgagggt gttgaagcgc atggaggacg
     1081 atttttcggg gctgcgaggc ggggtcaggc ggggtcctgc ccagactcga tggcgtccat
     1141 ccgcgctttg atgcgggcgg cgtcctcgta gcgctcgtcg cgcacggcct gctcgagttg
     1201 ggcgcgcagt tcggggaggc tggctttttt cctggctttg gcggggggcg gcggcgcggg
     1261 ggcggaggac tccgggaagg caacgggggc ggcctggggg gcggcaatgt ggaggtggcg
     1321 gggctttttg ccggtgtgct gggtgccttt gtgcatggat ttgagcaggc cctcaatgtg
     1381 ctggcggaag gtggtgtagc attcggggca gcccatgcgg ccgatttttt tgagctgggc
     1441 ggaggtgaag ccgcaggcgg ggcaggcggt ctcgatctcg ctgtgcggat gcgggggggt
     1501 gaggaaggct tcggcgagtc cgaagccggt ctcgtcggtg acgcctttgg ctttggagca
     1561 ggcgtcgcac aagttcacgg tggtcatctg gccgttgatg atctgggtga ggaacacggt
     1621 ggcctcggct tcgcaaacgt cgcagttcat gggtgggggg atttttcggg tgccgccgga
     1681 agggttacgc gcgcgctgcg cgccgggtgc tgacttatgg cagggcggcg gatgcgtcaa
     1741 ccacggggcg ggcagtgcgg agggacgggg cggtggcgga gcggaaggaa gcagggagtg
     1801 cttgattgtc gggttatatt atggaaatta taaggctgac tgcatgatgg accctcccga
     1861 tgaaactcca cgcaagccca gggtgcgcct gacaccctgg cgcgggccgt ttcatttcct
     1921 gctgctgctg gccaccagca gtgtgacgct gctgctgccg ctgctctacg cgctgctgtt
     1981 gctggcgctg ggcttccatg tgggagcgct ggcttttggc tggatcgagc atctggcgct
     2041 ggcggagacg cgggcatggc gggagctgct ggtcattccc tacatcgcgg tgggggcggt
     2101 gacgtgggtc ttcatgctgc gcccgctgct gccgcgcccg cgctcgacgc gggtggcgat
     2161 gcaggtgacg ccgacgagcc agcctgcgct cttcgaactg gtggacgagc tgtgctggaa
     2221 cctgcgcttg gagccgccgg aggagatctg gctggacacg acgacgagcg tgcgcacgag
     2281 cctgcgcaac ggggtgtccg gcgcgctgac aggggagtcg gtgatccatg tcgggctgcc
     2341 ggtggtgtcg gtgctggggg cgcgggagtt tgcggcgctg gtggcgcggg agctggcgat
     2401 gaacacgggc gggctgggca cgacattttc ccacctggtg agggagctga acacctggtt
     2461 ctaccgcgcg ctgcacgagc gcgacccgtg ggagctgcgg ctgcgccaga agcaggaaaa
     2521 ggagacgcgc tggcaggcgg gctgccgggt ggcgatctgg ctgtggatgg cggcggcgaa
     2581 gctgcccttc gccttcttcg tgctggcggc gcgggtgatc agcgcggcgg cgctcatgcg
     2641 cctgggttcg ggcgcggaca aggcgggcgt gaatctggcg ggacgcgcgg catggcgcgc
     2701 gatgcaggcg aagctgcggc tgctggacgc ggcgtgggag tcggcgcggg tggaggtgcg
     2761 gcgcggcatc tcgcagcacc ggctgccgga caatctctcc ctgctcatcg cccgccatgt
     2821 ggcgcgcgcc gccggggacg ggtgcccggc tgagcctgcg ggcggcccgg agccgaaagg
     2881 cgcgcgcatc atcagcgggc tggaggaaaa cgcgcccgcc gccgtgctgc tgcggggctt
     2941 tgtggacctg tcacggcagt tgacgtcttt ttattaccag cacgacctgg gcatccagat
     3001 tcacgagcac aggctggtgg ccgaggagga ggtcattcat caaaaccgcc gcgaggacga
     3061 gagcctggtc atcatccgcc gttacttcag cgggctggcg catccggagc gcgccatgtg
     3121 cgggcatgcg ccgacgcacg ccttttcccc aggcagggag cggctcgaga atgagatccg
     3181 ccgggtgcgc caggacatcc gcgagtgggg cagccgcctg aagcccgcgc tccaggagtg
     3241 gaacttggcc tggcagcgac ggcgagacct ggaggccgcc gccgtgctga gcctgggcgg
     3301 cttcaatgtc tcgcgcatcc agtttggcac ggaggacacc agcccgcagt cactgcgcca
     3361 ggaggccgcg cgccagcgca tgctgatgga gcacatggag acggcgctga cggctttcga
     3421 ggccaaaatg gaaagccgct ttgccgccgc gctcggcctg ctgtggtgga gcgcgcccga
     3481 cgagctggac gcgcgcctca tcgaacgccg gcaggacctg ccgcattggg tgggactgta
     3541 tgaggccatg gccggggcgc tgcccagctt ccgcgagttg ctcaccacct ttttcgcctt
     3601 ccagacgctg ggcgcgcgct tctccagcct ggatgacgcc ggaccgatgt tccaggcgct
     3661 ccagtccgtg gtgccaaaga tgatcaacct cacgcgccag atcctctccg ccatggacgg
     3721 cgcgcaatac gccttcacct ccaaccgccg ccccatgccg ctggtcgcgc acatgctgcc
     3781 cggcaggctg cccgagccgg tgggcatttc catggacccg gcagccgccg ccgacaccaa
     3841 ggcgctctcc gccaaaatgg ccgccgacgc ctccgaggtc gtcgccccgt ttgtggaccg
     3901 cttcatgcag ctctggcaca agtcctacgc atggctggcc gagtccgccg agctgaccga
     3961 gatgcatttc ctcggcccca tgagcctgag cgcgggcacc gccgagctgc tgacacccga
     4021 ggacatggcc cggcccgcgc caggcgccgg cgcggcggcg gcgaaaacga aagcggcgtg
     4081 agcaaaaagc cccggaaaaa agcacgctgg cagcgcgcgt taaaccgtgc caatcgtccg
     4141 catgtcacgc tgcctgtcct ttgccgccac cgggaatccc gccgaggtgc tcgaactcat
     4201 agagcggccc ctgcccgcgc tcggcccgca tgacgtgcgc gtccacatgc gctactcccc
     4261 cgtcaatccc gcggatctca atttcatcga gggcacttac ggacgcccgg cgcatccgcc
     4321 cgccattcca gggcacgagg gctgcggcga ggtggttgag accggagcgg aggtggcctc
     4381 tctgaaaaac ggggacgtgg tcatcccgct gctcggcgcg ggctgctgga cgcggcacat
     4441 gacagcggcg gagcattttt tcgccaaact gccgccgggc gtggaccgtg tgcaggcgtc
     4501 catgctgcgc atcaatccgg tcaccgcatg gcacctgctg caccacttca tcccgctcga
     4561 agatggcgcg tgggtggcgc aaaacgcggg caactccggc gtcgggcgct gcgtcatcca
     4621 gatcgcgcgc cagctcggtt tgaaaacggc cagctttgcg cgccggccgg agctggtgga
     4681 ggagctgcgc gcgctgggcg cggacgcggt ttacctcgat gacgaggccg gcctggctgc
     4741 cgccaaaaaa gacctccacg gcagcgagat ccgcctcgcc gccaatgccg tcagcggtga
     4801 cagcgccatc cgtctcatgg acctgctctc ccccggcggc acgctggtga cttacggagc
     4861 catgagccgc cgcagcctca aagtgccgaa caaatttctt atcttcaaag acctcgtcct
     4921 gcgcggcctg tggatcaccc gctggctgga gcacgccggc acggacgagc tgttcaaagc
     4981 gctcgacccc ctggccaaaa tgatcctcca tggcgagctc gtccagcccg tggacgaaat
     5041 catccccatg agcgagcacc gccgcgccat gaggcgcgcc caggagaccg ggcgcacagg
     5101 caagatcatc cttgatctgg catgagcgcg gacacacacg ccatcttcaa agtcatgagg
     5161 ccctttttgc tcatccccct gctgctctcc accaccgcgc ccctgcgcgc cgcgccggag
     5221 atcatctcac cccaggtcaa ggccagcgtg gacaaggccg tggcctggct gctgcgccag
     5281 cagcacccct ccggctactt ttccgccgag aaaaccgaca agcagcccca gcgcggcgac
     5341 atccccagcc attccgccgc catgaccgcg ctcactctca tgggcctggc ctccgtcggt
     5401 cacctgcccg gcaatcccgg ctccgagggc gaggccgccg cgcgcgcgct gaagttcatc
     5461 gtggaccatg tcgagccgga tgaaaacggc tacctcggcc gcaccgaccg cagccgcatg
     5521 tacgggcacg gcatcatcac cctcatgctc accgaaatgc tcggcatggc cgccgacgag
     5581 gagaccgacc gcaaagcgcg cgccatgact gccaaagcca tccagctcat catccgagcc
     5641 cagcagacgc ccaagagcga ggccaaccgc ggcggctggc gctacgaacc cagcagcagc
     5701 gacagcgaca tctccgtctc
//
LOCUS       DAOMYJ010000284         5619 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Prosthecobacter sp. isolate AMR_MDS_5629
            NODE_4946_length_5619_cov_18.029655, whole genome shotgun sequence.
ACCESSION   DAOMYJ010000284 DAOMYJ010000000
VERSION     DAOMYJ010000284.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867850
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prosthecobacter sp. (wastewater metagenome)
  ORGANISM  Prosthecobacter sp.
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Verrucomicrobiaceae; Prosthecobacter.
REFERENCE   1  (bases 1 to 5619)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5619)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.30
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:34:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,742
            CDSs (total)                      :: 4,684
            Genes (coding)                    :: 4,676
            CDSs (with protein)               :: 4,676
            Genes (RNA)                       :: 58
            tRNAs                             :: 55
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5619
                     /organism="Prosthecobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4946_length_5619_cov_18.029655"
                     /isolate="AMR_MDS_5629"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1965333"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..918)
                     /locus_tag="PLP58_20060"
     CDS             complement(<1..918)
                     /locus_tag="PLP58_20060"
                     /inference="COORDINATES: protein motif:HMM:NF024994.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HRJ10887.1"
                     /translation="MENLEAFFSPSRVAVIGATEKAGSVGRAVVENLRGFHGAVHLVN
                     RKHPEVLGIKTLARVSDLPAGTDLAVVVTPAPAVPEILRECAAAGIPAAVVISAGFKE
                     AGAAGRELEREVLEIARAASMRVIGPNCLGVMVPGTGLNATFAAAMAMPGNIAFLSQS
                     GALCTAILDWSFSAGMGFSAFVSTGSMLDAGWADLIAHFADDPRTAVIVLYMESVDDA
                     PAFLGSARRAALKKPLVVLKAGRTEAAARAAASHTGALTGSDSVFDAAFARAGVLRVD
                     SIAELFQTAEVLACRRPPRGRRLAIVTNAG"
     gene            complement(1018..2520)
                     /locus_tag="PLP58_20065"
     CDS             complement(1018..2520)
                     /locus_tag="PLP58_20065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009963974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ10888.1"
                     /translation="MKKLILAFLLPATATLAQTAVPVLKAGFAERDITPGIGMEQPGG
                     YGKSFHQKFHDACKVRAAFFDDGKKRVALVGTDTLVIPRQVVLDARARIEKELGIPGD
                     CVLVGASHSHSSGPVGMVLPGEYDAASDLVKRLAYEESSCADEGFLMKVTHEIVEGVR
                     AAARGAVPAQIGCGFGHEDKVAFNRRLRMKNGQSWSHPGQGNPDMLEYAGPIDPQVGV
                     IGAWDAEGNLLGTIVNYACHATTNPGGISANWIYYLEKTIQGALESRAPVVFLQGACG
                     DVTQVDNLSPHRRPGAEEWARFVGGRVGAEAVKVLLSMPRTGEAVLDSRQKVLSIPRR
                     KPAPARVQGSLEIIQAGRPQGDTSGYLFAKEILMLDHLCKVAPEVECEVQVIQVGPAA
                     FVTNPAEYFVQFGLDIKKGSPFAYTFPVELANGIVGYVPTEEAFGPNGGGYETRLTAY
                     SNLVITAGRQMADTGIALTKDMQPGPAPEHPKAPPFGRAWTYGNVPPELE"
     gene            complement(2682..3968)
                     /locus_tag="PLP58_20070"
     CDS             complement(2682..3968)
                     /locus_tag="PLP58_20070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009961111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HRJ10889.1"
                     /translation="MNLLQSLDYSVLQQCMHCGMCLPTCPTYVETKLERNSPRGRISL
                     MRSIADGDLGVTRAFADEMYYCLGCLACQTACPAGVKYAELFETSRAEVERAGVAAHD
                     ERDFWRWLTLDVLFMHPRLLRLAGWGLRLWQSTGLDRVLRRFHFLGLLPKKLRALEPQ
                     TPRMAQAFSDSLIDPVETPPEKRYRVGLLTGCVQDLAFSGINRDTADVLLANGCEVVT
                     PRVQHCCGSLHAHNGAVEQARELARRQLDSFDLDQFDAVITNAGGCGSHLKSYGHLLH
                     DDPFYAAKARQWDAKVKDIHEWLVHTGIRAPEHGAGVCEVTYHESCHLCHGQKVTSQP
                     RQVLQAIPGLVLKELPESNWCCGSAGIYNITQPEQSAKLLRRKVGNIRATRAPVVATS
                     NPGCHMQLVNGLDGSAEVTQPVTLLARAYRGGASGA"
     gene            complement(4044..>5619)
                     /locus_tag="PLP58_20075"
     CDS             complement(4044..>5619)
                     /locus_tag="PLP58_20075"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF012403.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HRJ10890.1"
                     /translation="ATRPAAGRDGRLHLDKLRAAQLASLEGLPLQMPAELTRLRERLA
                     GFTSMAFVEPPAGLRATLRPYQTEGLSWLQFLREFGLHGILADDMGLGKTLQTLAHLL
                     AEKEAGRMDRPCLILAPTSVLRNWAREAAKFTPDLRVLVLHGEERRGDYFRIPKHDLV
                     ITSYPLLVRDADRLLGIQWHLAVLDEAQHIKNPRSKAAQTCRQLKARHRLCLTGTPME
                     NHLGELWSLFDFLMPGLLGDADSFRELYRNPIERGQDDERRQQLAARLQPLMLRRTKD
                     AVAKDLPPKTEILHTIELDKTQADLYETIRAAVDKRVKEAISAQGLDRSQIIVLDALL
                     KLRQVCCHPRLLKTESAQHSVVSAKTAYLMEEMLPELLDEGRSILIFSQFTEMLALLE
                     KEIRGAGIRFVKLTGSTKDRETPVRQFQSGEARVFLISLKAGGSGLNLTAADTVIHYD
                     PWWNPAAEAQASDRAHRIGQTRPVFIHKLICQDTIEERILEMQKSKAALVEGLLSGRA
                     ENLHLTREDLQRLLEA"
BASE COUNT          863 a         1758 c         2076 g          922 t
ORIGIN      
        1 gcccgcgttg gtgacgatgg ccagccgccg tccgcgcggc ggtctgcggc aggccaggac
       61 ctcggcggtc tggaaaagct cggcaatgct gtccacgcgc agcacgccgg cgcgagcgaa
      121 ggcggcgtca aagaccgagt cgcttccggt cagggcgccg gtgtgggagg cggcggccct
      181 cgcggcggcc tcggtgcggc cagccttgag cacgacgagc ggttttttca aagccgcccg
      241 ccgcgccgaa ccaagaaatg cgggcgcgtc atccacggac tccatgtaga gcacgatgac
      301 cgccgtgcgc ggatcatccg cgaagtgcgc gatgaggtcc gcccagcccg catcgagcat
      361 cgaacccgtg gagacaaagg cgctgaagcc catgcccgcg ctgaagctcc agtccaggat
      421 ggcggtgcag agcgccccgc tctggctgag aaaggcgatg tttccaggca tcgccatggc
      481 ggcggcgaaa gtggcgttca ggccggtccc aggcaccatc acgccgaggc agttcggacc
      541 gatgacgcgc atggacgcgg cgcgcgcgat ctccagcacc tcacgctcca gctcgcggcc
      601 cgcggctccg gcctccttga aacccgccga aatgacgacg gccgccggaa tgcccgccgc
      661 cgcgcactcg cgcaggattt cgggcactgc cggcgcggga gtcacgacga ccgccaggtc
      721 cgtcccggcg ggcaggtcgg agacgcgcgc gagtgtcttg atgccgagca cctcgggatg
      781 cttgcgattc accagatgca ccgcgccgtg gaagccgcgc agattttcca ccaccgcgcg
      841 ccccacgctg ccggccttct ccgtggcgcc gatcacagcc acacgactcg gagaaaaaaa
      901 ggcttcaaga ttttccatga catccagtct gccgggcaca aagccgcgag gcaagccgcc
      961 cggaaggcgt ctttcgcaac tctccaccga aacacagcgg tatttggacg ggaagcgtca
     1021 ttccagctcg ggcggcacgt tgccgtaggt ccaggcgcgg ccgaagggcg gggccttcgg
     1081 gtgctcgggg gcgggaccgg gctgcatgtc cttggtcaag gcgatgccgg tgtcggccat
     1141 ctggcggccg gcggtgatga cgaggttgct gtaggcggtg aggcgcgtct cgtagccgcc
     1201 gccattcggg ccgaaggcct cctccgtggg cacatagccg acgatgccgt tcgccagctc
     1261 cacggggaag gtgtaggcga aggggctgcc ttttttgatg tccagcccga actggacgaa
     1321 atactcggcg gggttcgtga cgaaggcggc ggggccgacc tggatgacct gcacctcgca
     1381 ctccacctcg ggcgcgactt tgcacaggtg gtcgagcatg aggatctcct tggcgaagag
     1441 gtagccggag gtgtccccct gcgggcggcc ggcctggatg atctccaggc tgccctggac
     1501 gcgggcgggc gcgggcttcc ggcgggggat ggagagcacc ttttgccggc tgtcgagcac
     1561 ggcctcgccg gtgcgcggca tggagagcag cactttcacc gcctcggcgc ccacgcggcc
     1621 gccgacgaac cgcgcccact cctccgcgcc ggggcggcgg tgcgggctga gattgtccac
     1681 ctgcgtcaca tcgccacacg cgccctggag gaagaccacg ggcgcgcggc tctccagcgc
     1741 tccctggatg gtcttttcca ggtaataaat ccagttcgcc gagatgccgc cggggttcgt
     1801 ggtggcgtgg caggcgtagt tgacgatcgt gcccagcagg ttcccctccg cgtcccacgc
     1861 gccgatgacg cccacctgcg ggtcaatggg gccggcatac tccagcatgt ccgggtttcc
     1921 ctggccgggg tggctccagc tctggccgtt tttcatgcgc aggcggcggt tgaaggccac
     1981 cttgtcctca tggccgaagc cgcagccgat ctgcgcgggc accgctccgc gcgcggcggc
     2041 gcgcacgccc tccacgatct cgtgcgtgac cttcatgagg aagccctcgt cggcgcagga
     2101 ggactcctca taggccaggc gcttcaccag gtcggaggcg gcgtcatatt cccccggcag
     2161 caccatgccc accggcccgg aggagtggga gtgggacgcg ccgacgagca cgcagtcgcc
     2221 cggaatgccc agctcctttt cgatgcgcgc ccgcgcgtcg agcaccacct gtcgcggaat
     2281 gacgagcgtg tcggtgccca cgagagccac gcgctttttg ccgtcgtcaa aaaaggccgc
     2341 gcgcaccttg caggcgtcgt ggaacttctg gtggaagctc ttcccatagc cgcccggctg
     2401 ctccatgccg atgcccggcg tgatgtcgcg ctcggcaaag ccggccttca aaaccgggac
     2461 ggcagtctgc gcgagcgtgg cggtggcggg gagaaggaag gcgaggatca gctttttcat
     2521 gcgcgagggt tacgctacat gaaggtggaa tcgcgcacgc gcggccctgg tgaggcgcgc
     2581 gcccgtagag gaagacgtga gtcttccctt tgacgcgtcg agtggagaga agactcgcga
     2641 gccaacgtga gctctctccc aaggcaaact cacgtttgcc tctacgcgcc cgaagcgccg
     2701 ccccgatacg cccgcgccag cagggtgacg ggctgggtga cttcggcgct gccgtcgagg
     2761 ccgttgacga gctgcatgtg gcagccgggg ttcgaggtgg cgacgacggg ggcgcgtgtg
     2821 gcgcggatgt tgcccacttt gcgccgcagg agcttcgcgg actgctccgg ctgggtgatg
     2881 ttgtaaatgc cggcgctgcc gcagcaccag ttgctctccg gcagctcttt caagacgagg
     2941 cctgggatgg cctggagcac ctggcggggc tggctggtga ctttctgccc gtggcagagg
     3001 tggcagcttt cgtggtaggt gacctcgcag acgcccgcgc catgctccgg agcgcggatg
     3061 ccggtgtgga cgagccattc gtggatgtcc ttcaccttcg catcccactg ccgggctttg
     3121 gcggcgtaaa aggggtcgtc gtgcagaagg tggccgtagc ttttgagatg ggagccgcag
     3181 ccgccggcgt tggtgatgac ggcgtcgaac tggtcgagat cgaagctgtc gagctgccgc
     3241 cgcgccagct cgcgcgcctg ctccacggcg ccattgtgcg cgtgcaggga gccgcagcag
     3301 tgctggacgc gcggggtgac gacctcgcag ccattggcca ggaggacatc ggcggtgtcg
     3361 cggttgatgc cggagaaggc caggtcctgc acgcagccgg tgagcaggcc gacgcggtat
     3421 cttttttccg gcggtgtctc cacgggatcg atgagggaat ccgagaacgc ctgcgccatg
     3481 cgcggtgtct gcggctccag cgcgcgcagt tttttgggca gcaggccgag gaagtgaaag
     3541 cggcgcagca cgcggtccag cccggtgctc tgccacaggc gcaggcccca gccggccaga
     3601 cgcagcaggc gcgggtgcat gaagagcacg tcgagcgtga gccagcgcca gaagtcgcgc
     3661 tcgtcatggg ccgcgacgcc ggcgcgctcc acctcggcgc gggaggtttc gaaaagctcg
     3721 gcgtatttca cacccgccgg gcaggccgtc tggcaggcca ggcagccgag gcagtagtac
     3781 atctcgtcgg caaaggcgcg cgtgaccccc aggtcgccgt cggcgatgga gcgcatgagg
     3841 gagatgcgcc cgcgcgggct gttccgctcc agcttggtct ccacataagt cgggcaggtg
     3901 ggcaggcaca tgccgcagtg catgcactgc tggagcacgg agtagtcgag ggattggagg
     3961 aggttcaccg gcggctatga agggtggatt cccgcgcttt ggcaaccgac ttcgaaggca
     4021 tgattgaaac gcgccgggcg gcctcacgcc tcgagcagcc tttgcaggtc ctcgcgggtg
     4081 aggtggaggt tttcggcgcg gccggagagc aggccctcga cgagggcggc cttgcttttc
     4141 tgcatctcca ggatgcgctc ctcgatggtg tcctggcaga tgagcttgtg gatgaagacg
     4201 ggccgggtct ggccgatgcg gtgcgcgcgg tcgctggcct gggcttcggc ggcggggttc
     4261 caccacgggt cgtagtggat gacggtgtcg gcggcggtga ggttgaggcc ggagccgccg
     4321 gccttgaggc tgatgaggaa gacgcgcgcc tcgccgctct ggaactggcg cacgggagtc
     4381 tcgcggtcct tggtggagcc ggtgagtttg acaaaacgga tgcccgcccc cctgatctcc
     4441 ttctccagca gggcgagcat ttcggtgaac tgggagaaga tgagaatgga gcgcccctca
     4501 tcgagaagct cgggcagcat ttcctccatg agataggcgg tctttgccga gacgacggag
     4561 tgctgggcgg actcggtctt caaaagccgc gggtggcagc agacctggcg gagcttgagc
     4621 agggcgtcga gcacgatgat ctggctgcgg tccaggccct gggcgctgat ggcctccttc
     4681 acgcgcttgt ccacggcggc gcggatggtc tcgtagaggt cggcctgggt tttgtccagc
     4741 tcgatggtgt ggaggatctc ggtcttgggc ggcagatcct ttgccacggc gtccttggtg
     4801 cggcggagca tgagcggctg gaggcgcgcg gcgagctgct ggcggcgctc gtcgtcctgg
     4861 ccgcgctcaa tggggttgcg atacagctcg cggaagctgt cggcgtcgcc gagcaggccg
     4921 ggcatgagga agtcgaagag gctccagagt tcgccgaggt ggttttccat cggcgtgccg
     4981 gtgaggcaga ggcggtggcg ggccttgagc tggcggcagg tctgggcggc tttgctgcgg
     5041 gggtttttga tgtgctgggc ctcgtcgagc acggccaggt gccactggat gccgaggagc
     5101 cggtccgcgt cgcgcacgag cagggggtag gaggtgatga cgaggtcgtg cttggggatg
     5161 cggaagtagt cgccgcgccg ctcctcgccg tgcagcacga gcacgcgcag gtccggggtg
     5221 aacttggcgg cctcgcgcgc ccagtttcgc aggacggagg tgggcgccag gatgaggcag
     5281 gggcggtcca tgcgcccggc ctctttttcc gccaggaggt gggccagggt ctggagtgtt
     5341 ttgcccaggc ccatgtcatc ggccaggatg ccgtgcaggc cgaactcgcg caggaactgc
     5401 agccaggaaa gcccctcggt ctggtagggc cggagcgtgg cgcgcaggcc ggcgggcggc
     5461 tccacgaagg ccatgctggt gaagccggcg aggcgctcgc gcaggcgcgt cagctcggcg
     5521 ggcatctgaa gaggcaggcc ttccagactg gcgagctggg cggcgcgcag cttgtcgagg
     5581 tgcaggcgcc cgtccctgcc ggcggcgggg cgcgtggcc
//
LOCUS       DAOMYJ010000285         5615 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Prosthecobacter sp. isolate AMR_MDS_5629
            NODE_4951_length_5615_cov_19.454496, whole genome shotgun sequence.
ACCESSION   DAOMYJ010000285 DAOMYJ010000000
VERSION     DAOMYJ010000285.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867850
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prosthecobacter sp. (wastewater metagenome)
  ORGANISM  Prosthecobacter sp.
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Verrucomicrobiaceae; Prosthecobacter.
REFERENCE   1  (bases 1 to 5615)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5615)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.30
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:34:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,742
            CDSs (total)                      :: 4,684
            Genes (coding)                    :: 4,676
            CDSs (with protein)               :: 4,676
            Genes (RNA)                       :: 58
            tRNAs                             :: 55
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5615
                     /organism="Prosthecobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4951_length_5615_cov_19.454496"
                     /isolate="AMR_MDS_5629"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1965333"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..517)
                     /locus_tag="PLP58_20080"
     CDS             complement(<1..517)
                     /locus_tag="PLP58_20080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ10891.1"
                     /translation="MTFPPRFQRLLLAALIWLGGAVSATAEWLVYELKFTPDSETVNF
                     SFYSSAYVIAPLNGGPVSIVLATEEGGQYYALAESSGKFFVAANAQARKAVFSALALK
                     GTSQAFYTASGHLNRSLLLKNAAGQTRTWRVAETLAGRLMSADDESERGPAEDGSYGV
                     VGGAVITGALRE"
     gene            complement(599..1393)
                     /gene="lpxA"
                     /locus_tag="PLP58_20085"
     CDS             complement(599..1393)
                     /gene="lpxA"
                     /locus_tag="PLP58_20085"
                     /EC_number="2.3.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009964190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008780 -
                     acyl-[acyl-carrier-protein]-UDP-N-acetylglucosamine
                     O-acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-ACP--UDP-N-acetylglucosamine
                     O-acyltransferase"
                     /protein_id="HRJ10892.1"
                     /translation="MIHPTAIIDPSAQIAADVEIGPYCIIGAGVDLGAGCRLQHHVTL
                     MGPAKIGPGNIFFAYGSIGQQTQDLKYKGEPTLLEIGEGNTFREFCTVHRATSPGDKT
                     VIGSRNHFLSYVHIAHDCVVGSHCVFSNNGTLAGHVIVGDHVILGGLSAVHQFCRIGE
                     RSIIGGCSKIVQDVPPYSTADGNPARARGLNIVGLQRAGFSREQIRALRHAFRKVYRS
                     GLNNAQAVEELRAGELTPEAARFTDFVATTKRGIIAGGKSADDAED"
     gene            complement(1447..2766)
                     /locus_tag="PLP58_20090"
     CDS             complement(1447..2766)
                     /locus_tag="PLP58_20090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009964189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008759 - UDP-3-O-[3-hydroxymyristoyl]
                     N-acetylglucosamine deacetylase activity [Evidence IEA];
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-3-O-[3-hydroxymyristoyl]
                     N-acetylglucosamine deacetylase/3-hydroxyacyl-ACP
                     dehydratase"
                     /protein_id="HRJ10893.1"
                     /translation="MSASDRQHTLAKAASITGTSLHTGEQVTLTLQPAPENFGFKFRR
                     IDLEDKPFIPALVEKVQKVERATTIAEGGVNVHTVEHVISALTGMGVDNAIIEMDANE
                     PPIVDGSSQPFVELIKKAGLQEQSEPRKIFEIREPIYQETRDGTILTIVPDKKFRVSC
                     TNVGPGGRFTQYLSLEITPETYEKEIAPARTFVYYEDIAPLMEKGLIKGGTLEAAVVV
                     RGETLLSKQPLRFENEFVRHKILDIVGDLMLSGKRIRGHVIAVRPGHGPNTEMARAIV
                     AQYNAMRAMVPPAVNIPGGEAVLDVNEVMNILPHRYPFLLVDRIVGFEGENKCRGVKN
                     VTINEPYFQGHFPGHPIMPGVLQLEAMAQVASIVLLRLPGNEGKIGYFMSANNVKWRK
                     PVLPGDTLFIETEVLKVKRSIAQAVGRCVVNGQVVSEADLMFSVVDR"
     gene            complement(2869..3420)
                     /locus_tag="PLP58_20095"
     CDS             complement(2869..3420)
                     /locus_tag="PLP58_20095"
                     /inference="COORDINATES: protein motif:HMM:NF019262.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ10894.1"
                     /translation="MIHRPILVLFSAALAVQAADFKKDIQPILEKHCYECHSEKTGKQ
                     KAGYVFDDLETLKLDINPKGAIVPGKPAESHVFVVVSDPNHEAHMPPKGNLSDREISK
                     LRDWITAGAHLTPEAAATAAAPAKPAVLDWTNFEGRTIKAGFVRLDGENVVLKMASGQ
                     EIPYPLAKLNEASRKQAQDSAAQ"
     gene            complement(3469..>5615)
                     /locus_tag="PLP58_20100"
     CDS             complement(3469..>5615)
                     /locus_tag="PLP58_20100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009960863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF1549 and DUF1553 domain-containing protein"
                     /protein_id="HRJ10895.1"
                     /translation="PAWVPGRPEESQLIQAVTGTHAEIAQMPYKRGPLDSAQIALLRQ
                     WVAEGAAAPAGETPSDDRHWAFVPPVKAVKSETRNSKPGTHPIDVFIRGKLAKAGLEP
                     SPRAAPETLIRRLHLDLTGLPPTPEEVESFAKDGDYEARVERLLASPAHGERWGRWWL
                     DQARYADSNGYSIDAPRSIWPYRDWVVAALNADMPFDRFTIEQLAGDLLPEATREQII
                     ATGFHRNTQINQEGGIDREQFRIESVFDRVGTTGTVWLGLTLACAQCHDHKFDALSQR
                     EFYEMFAFFNSQDEPALPVPQPGQDLEKLKSELAEVAGKLDAHVQANLERLREWEKDL
                     NADAKKLLSAAVTKTLAKPARKRTADDLRVLFKAMTGTGDPFFNPLHDRLRELEQTLK
                     SGVTTLVLKELPKPRKTTLFIKGDFTRPAEEVAPAVPAVLHPLKAENPTRLDLARWLV
                     SRDNPLTARVIVNRVWQQYFGRGLVETENDFGTMGTPPTHPELLDWLAVEFMDGGWSL
                     KHLHRVIVTSAAYQQSSARHPQAGGGRAGSADPNNDLLWRQNRLRLDAEIVRDASLAA
                     SGLLSPKMGGPPVYPPIPEGVMSLGQVKRAWTESKGDDRYRRGLYTFVFRATPPPSMS
                     VFDAPDGYSACTRRLRSNTPLQALTLLNDAGFVEFARALEKIIEREGVGRAFQRCTSR
                     LPDAEEKRALAALPPFEAARVMLNLDETITRE"
BASE COUNT          822 a         1743 c         1980 g         1070 t
ORIGIN      
        1 cctcgcgcag cgcgccggtg atgacggccc cgccgacgac gccgtaggag ccgtcctccg
       61 cggggccgcg ctcggactcg tcatcggcgc tcatgagcct gccggcgagg gtctcggcca
      121 cccgccaggt gcgggtctgg ccggcggcgt ttttcagcag cagggagcgg ttcaggtggc
      181 cggaggcggt gtaaaatgcc tgcgaggtgc ctttcaaggc cagggcgctg aagacggcct
      241 tgcgcgcctg cgcgtttgcg gccacgaaga acttgccgct gctctccgcc agcgcgtaat
      301 actgcccgcc ctcctcggtc gccagcacga tggacaccgg cccgccgttc aggggcgcga
      361 tgacataggc gctgctgtag aagctgaagt tcaccgtctc gctgtccggg gtgaatttca
      421 gctcatagac gagccactcc gcggtggcgg atacggcgcc gcccagccag atcagggcgg
      481 cgagcagcag gcgttgaaag cgcgggggga aagtcatgaa agcggatttt atagccgtgg
      541 tggacagggt tgtcgagccg ggcgttgaat gttcctcctt cggcggacgt tggccgcctc
      601 aatcctccgc gtcgtccgcg cttttgcctc ccgcgatgat gccgcgcttg gtggtggcga
      661 caaagtcggt gaagcgcgcc gcctccgggg tgagctcgcc ggcgcgcaac tcctccacgg
      721 cctgggcgtt gttcaagccg ctgcgataga ctttgcggaa ggcgtggcgc agggcgcgga
      781 tctgctcgcg gctgaatccg gcgcgctgga ggccgacgat gttcaggccc ctggcgcggg
      841 cggggttgcc gtcagcggtg gagtaggggg gcacgtcctg gacgatcttt gagcagccgc
      901 cgatgatgct gcgctcgccg atgcggcaga actggtgcac ggcgctcagc ccgccgagga
      961 tgacatggtc ccctacgatg acatgcccgg cgagggtgcc gttgttggag aagacgcagt
     1021 ggctgccgac gacgcagtca tgggcgatgt ggacgtagga gaggaagtgg ttgcggctgc
     1081 cgatgacggt cttgtccccc ggactggtgg cgcggtgaac ggtgcagaat tcgcggaagg
     1141 tgttcccttc gccgatttcg aggagggtcg gctcgccctt gtatttcaag tcctgcgtct
     1201 gctggccgat ggagccgtag gcgaagaaga tgttgccggg gccgattttg gcgggtccca
     1261 tgagggtgac gtggtgctgg aggcggcatc cggcgccgag atcgaccccg gctccgatga
     1321 tgcagtaagg gccgatctcg acatcggcgg cgatttgcgc ggaggggtca atgatggcgg
     1381 tggggtggat cacgcgggag ggtgtcagga tgaaaagagt tgctggcgcg gccatcgcac
     1441 ggcggttcag cggtccacga cgctgaacat gaggtcggcc tcggagacga cctggccgtt
     1501 gacaacgcac cggcccacag cctgggcgat gctgcgcttg accttgagca cttcggtctc
     1561 gatgaaaagg gtgtcgccgg gcagcacggg cttgcgccac ttgacgttgt tggcgctcat
     1621 gaagtagccg attttgccct cgttccccgg caggcgcagc aggacgatgc tggccacctg
     1681 ggccatggcc tcgagttgga gcacgccggg catgatgggg tggccgggga agtggccctg
     1741 aaaatagggc tcgttgatgg tgacgttttt cacgccccgg catttgttct ccccctcgaa
     1801 gccgacgatg cggtccacga gcaggaaggg gtagcggtgc gggaggatgt tcatcacctc
     1861 gttgacatcc agcacggcct cgccgccggg gatgttcacc gccgggggca ccatggcgcg
     1921 catggcgttg tattgggcga cgatggcgcg cgccatctcg gtgttcggtc cgtggccggg
     1981 gcgcacggcg atgacgtgcc cgcggatgcg cttgccggag agcatgaggt ctcccacgat
     2041 gtcgaggatc ttgtggcgga cgaactcgtt ttcaaagcgc agcggctgct tggagagcag
     2101 cgtctccccg cgcacgacca cggccgcctc gagggtgccg cctttgatga ggcccttctc
     2161 catgagcggg gcgatgtcct cgtaataaac aaaggtgcgc gccggggcga tctccttctc
     2221 ataagtctcc ggcgtgatct ccagggacag gtactgggtg aagcggcctc caggcccgac
     2281 gttggtgcag gagacgcgga attttttgtc gggtacgatg gtgaggatgg tgccgtcccg
     2341 cgtctcctgg tagatgggct cgcggatttc gaagattttg cgcggctcgg actgctcctg
     2401 gaggccggct tttttgatca gctcgacgaa gggctgcgag ctgccgtcca cgatgggcgg
     2461 ctcgttggcg tccatctcga tgatggcgtt gtccacgccc atgccggtga gcgcggagat
     2521 gacgtgctcg acggtgtgaa cattgacccc gccctcggcg atggtggtgg cgcgctcgac
     2581 cttctgcact ttctccacca gggcggggat gaagggcttg tcctccaggt caatgcgcct
     2641 gaatttgaag ccgaaattct ccggcgcggg ctgcagggtc agcgtcacct gctcgcccgt
     2701 gtgcagggag gtgccggtga tggaggcggc tttggcgagg gtgtgctgac ggtctgaggc
     2761 ggacattggg cagccgtttt agccgcgcgc gctttcaggg tcaaaggaaa atccagccgc
     2821 acggcggcgc cgggaaatgg acgggtccgg tcatgaggcc gggcctcctc attgcgccgc
     2881 gctgtcctgc gcctgcttgc ggctggcttc gttgagcttc gccagcggat acgggatctc
     2941 ctgcccgctg gccatcttga gcaccacgtt ttcgccgtcg agccgcacga agcccgcttt
     3001 gatggtccgg ccttcaaagt tggtccagtc cagcaccgcc ggcttggcgg gggcggcggc
     3061 ggtggccgca gcctcgggtg tcaggtgcgc gccggcggtg atccagtcgc gcagcttgga
     3121 gatttcacgg tcggaaaggt tgcctttggg cggcatgtgc gcctcgtggt tgggatcgga
     3181 gacgacgacg aagacatggc tttccgcagg cttgccgggg acgatggccc ctttcggatt
     3241 gatgtcgagc ttcagggttt ccaggtcgtc gaaaacgtag cccgcctttt gcttgccggt
     3301 cttctcgctg tggcactcgt agcagtgctt ttcgaggatg ggctggatgt cttttttgaa
     3361 atccgccgcc tgcacggcaa gcgcggcgga gaacaggaca aggatggggc ggtggatcat
     3421 ggtgcggatg gaacgcgcct gggcggcatt ccttggcgcg tttttcggtc actcgcgcgt
     3481 gatcgtctcg tccaggttca gcatcacccg cgccgcttcg aagggaggca gcgccgccag
     3541 cgcccgcttt tcctccgcgt caggcagccg cgaggtgcag cgctggaagg cgcgcccgac
     3601 gccctcgcgc tcgatgattt tttccagcgc gcgggcgaac tccacaaagc ccgcgtcatt
     3661 gagcagtgtc aacgcctgca gcggcgtgtt gctgcgcagg cggcgggtgc aggcgctgta
     3721 gccgtccggc gcgtcaaaga cgctcatgct cggcggcgga gtggcgcgga agacgaaggt
     3781 gtaaagcccg cgccggtagc ggtcgtcccc cttgctttcc gtccaggcgc gcttcacctg
     3841 gccgagggac atcacgcctt ccgggatggg cggatacacc ggcggaccgc ccattttcgg
     3901 ggagagcagg ccgctggcgg cgaggctcgc atcccgcacg atctcggcgt ccagccgcag
     3961 gcggttctgc cgccagagca ggtcattgtt ggggtccgct gatccggccc tgccgccgcc
     4021 ggcctgcggg tgcctcgcgc tgctctgctg gtaggcggcg ctggtgacga tgacgcggtg
     4081 cagatgcttc aggctccagc cgccgtccat gaactccacc gccagccagt cgagtagctc
     4141 cgggtgcgtg ggcggcgtgc ccatggtgcc gaaatcattc tccgtctcca ccagcccgcg
     4201 cccgaagtat tgctgccaca cccggttcac aatgacccgc gccgtgagcg gattgtcccg
     4261 gctcaccagc cagcgggcga ggtccaggcg cgtgggattt tcggccttca acggatgcag
     4321 cacggcgggc acggcgggcg cgacttcttc ggcggggcgg gtgaagtcgc ccttgatgaa
     4381 aagcgtggtc ttgcgcggct tcggcagctc tttgagcaca agcgtggtga cgccgctctt
     4441 gagggtctgc tccagttcgc gcaggcggtc gtggagagga ttgaaaaacg ggtcgcccgt
     4501 gccggtcatg gccttgaaga gcacccgcag gtcatccgcg gtgcgtttgc gcgcgggttt
     4561 cgccagcgtc ttcgtcacgg cggcggaaag cagctttttc gcgtcggcat tgaggtcctt
     4621 ttcccactcg cgcagacgct ccaggttcgc ctggacatgg gcgtccagtt ttccggccac
     4681 ctcggccagt tcggacttga gcttttccaa gtcctgcccc ggctgcggca ccggcagcgc
     4741 cggctcgtcc tggctgttga agaaggcgaa catttcatag aactcgcgct ggctgagggc
     4801 gtcgaacttg tggtcgtggc actgcgcgca ggcgagggtc aggcccagcc acacggtgcc
     4861 cgtcgtcccc acccggtcga agacgctctc aatgcggaac tgctccctgt ctatgccgcc
     4921 ctcctggttg atttgcgtgt tgcggtggaa accggtggcg atgatctgct cccgcgtcgc
     4981 ctccggcagc agatcgcccg ccagttgctc gatggtgaac cggtcaaagg gcatgtccgc
     5041 gttcaaggcg gccaccaccc agtcgcggta gggccagatg ctgcgcggcg cgtcaatgct
     5101 gtagccgttg ctgtccgcgt agcgcgcctg gtccagccac cagcgtcccc agcgctcgcc
     5161 atgagccggc gaggccagca gccgctccac gcgcgcctca tagtccccgt ccttggcaaa
     5221 actttccacc tcctccggcg tcggcggcag tccggtgagg tcgaggtgca gccggcggat
     5281 cagcgtctcc ggagccgcgc gcggcgaggg ttccagcccc gcttttgcca gcttcccacg
     5341 gatgaagacg tctatcggat gcgttcccgg cttcgaattt cgagtttcgg atttcaccgc
     5401 tttcaccggc ggcacaaagg cccagtggcg gtcgtcactc ggcgtctcgc cggccggggc
     5461 cgccgccccc tccgccaccc actggcgcag cagcgcgatc tgcgccgagt ccagcggccc
     5521 gcgtttgtag ggcatctgcg cgatctccgc gtgcgtgccc gtcaccgcct ggatgagctg
     5581 gctttcctcc ggcctgcccg gcacccaggc cgggc
//
LOCUS       DAOMYJ010000286         5536 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Prosthecobacter sp. isolate AMR_MDS_5629
            NODE_5029_length_5536_cov_22.231710, whole genome shotgun sequence.
ACCESSION   DAOMYJ010000286 DAOMYJ010000000
VERSION     DAOMYJ010000286.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867850
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prosthecobacter sp. (wastewater metagenome)
  ORGANISM  Prosthecobacter sp.
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Verrucomicrobiaceae; Prosthecobacter.
REFERENCE   1  (bases 1 to 5536)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5536)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.30
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:34:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,742
            CDSs (total)                      :: 4,684
            Genes (coding)                    :: 4,676
            CDSs (with protein)               :: 4,676
            Genes (RNA)                       :: 58
            tRNAs                             :: 55
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5536
                     /organism="Prosthecobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5029_length_5536_cov_22.231710"
                     /isolate="AMR_MDS_5629"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1965333"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(32..1495)
                     /locus_tag="PLP58_20105"
     CDS             complement(32..1495)
                     /locus_tag="PLP58_20105"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase"
                     /protein_id="HRJ10896.1"
                     /translation="MKFSLLLCTLALCQTLNAAGKPGILWLTSEDHSPHMGCYGDKIA
                     RTPNVDALAAKGMLFQKAWSVAPVCAPARTAIISGIYPSSSGGLHMRSMVSLPEDVRM
                     YPAILREAGYYCTNNSKTDYNLRETGKVWDESSNKAHWKNRAEGQPFFAIFNSTKSHE
                     SQIRTRPHTQITKPDDVRVPAYHPDTPEVRQDWAQYYDKVSEADADAGRHLKELEEAG
                     LAEDTIVFYYADHGSGMPRGKRWPSNTGLHVPFVVYFPEKWKHLAPKEYMPGGKSDRL
                     ISFVDLAPTLLSLAGIEPPKWMQGRAFAGPYQSEPQPFIFGERGRMDERMDLVRSVTD
                     GRYVYLRNYHPHVSQAQRVAYQFETPTTRVWHDLYTQGRTNEAQSRFWKTPKDTEELY
                     DLKSDPDEVLNLAQSPEHRAILEKLRAAQREHLARVRDICFLPEREIHTRSEGGTPYE
                     TARDERKYPFEKILAAAELAAGMDSTASGELARTQPY"
     gene            complement(1583..4168)
                     /locus_tag="PLP58_20110"
     CDS             complement(1583..4168)
                     /locus_tag="PLP58_20110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ10897.1"
                     /translation="MNAPEAPLARHRRLIAENDALILGPSLDNGREITARRTAIHLDA
                     IAAWAAREWAERGCGRPFAVAAIGGTGRHEMTPCSDLDVAFLCDEAPDQSVFLCEMHR
                     QTNGSREFLDEHGFTFHAATYQLDYVADLDAKAVTALLDLSPVFDPHDLTGRFRERLR
                     VNHDAFAHFLHISEFWRARGGMHEDAHHAGKLDGFDIKAEALRDFQAGVWTLGGRRFR
                     HSHEVHAILPPEDMAAYHLLLRVRAFVQLRRGTRMEPLPNGLHIEDRMRFEDFLAFGD
                     LLGPDAAERDRFEFAAHARAALLNARRRVTRFARATIGRELLEGREVGRGIVYGMGGM
                     RHTTAPGCVSPEEKSRAALDLLLEAQRHSLPIDPAELDSAFKDIENWLVPVPELGALF
                     YEPRGSLASSLAHLARFPGAEERLFPGHAAFEVSVDERVMTEKKTLRGALLVEKFISL
                     EKRRAAGLDLSETRAEGLPVDINLLDEVETARAAPDTMAAVKLALKIKRLPLMADDAR
                     RRADTRLSLGERYSSGFSGVPVEDYLERCFSNAGFLPETLELTRFLITHRRLLEDLVA
                     DGVNDKSKVDRLASVCGGTSRLRALFVFTRADRAGWKDNVTDPGNWFAIRELYKMTWE
                     RFHPPGNPLGILDQMLAGGMPEEDAEVMRDLGVSFFGGAYARHTSRFSGHLAALHQKR
                     ETEPLLRMLHDGASIILGIAANNIQGLAAAICHTLWTNGVPVFRSHLFSATNLGLALD
                     FFHLRPGTAQPGREVLEKMRREIAAGPPSRAALPALPVSNMVMERRDSKVMRLRVWSP
                     ETSGGLLCMMTGLLYHALDAGIYALDSRAETDGTRLTILHSLPSRMTPENALARLRAE
                     TSLVA"
     gene            complement(4177..5319)
                     /locus_tag="PLP58_20115"
     CDS             complement(4177..5319)
                     /locus_tag="PLP58_20115"
                     /inference="COORDINATES: protein motif:HMM:NF002872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HRJ10898.1"
                     /translation="MNPLFEKRPVLIAFSGGDSPGMNPFMRAAARLGGNLHDVPVIGV
                     MEGYAGLVRTLARLESGEVTLDSLREEILHRPGIFGFHAKNQHLVHLDPESVSGLVVR
                     GGIKLRSARCDEFRDDGIRARVIQMLRGLGFRAVIVCGGDGSLAGARRLHEESGIQVV
                     GVPGTIDNDLPVTDWALGVQSAIENILQEVTKIKDTARSHRRVMVLETMGRGCGELAL
                     LSAIASGAEYAIIPEDGFLSGDRIRKLAGEIEQSHARGRTHSIVLVAEGVRMDRHKWE
                     SPAWMVAMRLGECFKHGPFKEIETRATVLGYVQRGGSPAAFDGILAAEFAGTAWQAIM
                     GTGASGVTVVRNGGHKLAPFDWQPDSLERAARLQKLRETHRALSGY"
BASE COUNT          904 a         1637 c         1983 g         1012 t
ORIGIN      
        1 cctgttcggc actggaatga gccataattg ttcaataggg ctgcgttctt gccagctcgc
       61 cgctggcggt ggagtccatg ccggcggcga gttcggcggc ggcgaggatt ttttcaaaag
      121 gatactttct ttcgtcacgc gcggtctcgt aaggcgtgcc gccctcggag cgggtgtgaa
      181 tctcgcgctc tggcaggaag caaatgtccc gcaccctggc cagatgctcg cgctgtgcgg
      241 cgcggagttt ttcgaggatg gctcggtgct ccggggattg ggcgaggttg agaacttcgt
      301 cggggtcgct tttcaggtcg tagagttcct cggtgtcttt gggcgtcttc cagaaacggg
      361 actgtgcttc attggtcctg ccctgggtgt aaagatcgtg ccagacgcgg gtggtgggcg
      421 tctcgaattg ataagccacg cgctgggcct gggagacgtg gggatggtag tttcgcaaat
      481 acacataacg cccgtcggtg acgctgcgaa cgaggtccat gcgctcgtcc atgcgcccgc
      541 gctcgccgaa gatgaagggc tgtggttcgc tttggtaagg accggcaaaa gcgcggccct
      601 gcatccactt cggcggttca atgccggcaa ggctgaggag cgtgggcgct aggtcaacga
      661 agctgatgag acggtctgac ttgccgccgg gcatgtattc cttcggtgcc agatgtttcc
      721 acttctcggg gaaatacacg acaaaaggca catgcagccc cgtgtttgaa ggccagcgct
      781 tgccgcgggg catgccgctg ccgtggtcgg cgtagtagaa gacgatggta tcctccgcga
      841 ggccggcctc ttcgagttcc ttcaaatggc gtcccgcgtc ggcgtcggcc tcgctgactt
      901 tgtcataata ctgcgcccag tcctggcgca cttcgggggt gtctggatgg taggcgggca
      961 cgcgcacatc atcgggcttg gtgatctgcg tgtgcgggcg ggtgcggatc tggctttcgt
     1021 ggcttttggt ggagttgaag atggcgaaga agggctggcc ttcggcgcgg tttttccagt
     1081 gcgccttgtt ggaggactca tcccagacct tgccggtctc gcgcaggttg tagtcggtct
     1141 tgctgttgtt cgtgcagtaa tagccggcct cgcggagaat ggcgggatac atcctcacgt
     1201 cctcgggcag ggacaccatg gagcgcatgt gcaggccgcc gctgcttgag ggatagatgc
     1261 cggaaatgat ggcagtgcgc gcgggcgcgc aaacaggagc cacggaccag gctttttgga
     1321 aaagcatgcc cttcgccgcg agcgcatcca cattcggagt gcgggcgatt ttgtcaccgt
     1381 agcagcccat gtgcgggctg tggtcctcgc tggtgagcca aaggatgcct ggcttgccgg
     1441 cggcgttcag tgtctggcag agagcgaggg tgcaaagcag gagcgaaaac ttcatggtga
     1501 gaggggacaa cgcggcaagg cagcctggat ttcaaggata tggcggctga tttcgggcga
     1561 tgcccggcga tgccggtgtg cttcatgcca cgagactggt ctccgcgcgc agcctggcga
     1621 gggcgttctc aggggtcatc cgcgaaggca ggctgtgcag gatggtgagg cgtgtgccgt
     1681 ctgtctcggc gcgcgagtcg agggcgtaaa tgccggcgtc caacgcatgg tagagcagtc
     1741 cggtcatcat gcagagaagg ccgccggatg tctcggggga ccagacgcgc agacgcatca
     1801 ctttggagtc gcgccgctcc atgaccatgt ttgaaacggg cagcgcgggc agcgcggcgc
     1861 gggaaggcgg tccggcggcg atctcccggc gcattttttc cagcacctcc cggccgggct
     1921 gggcggttcc cggccggagg tggaagaagt ccagcgccag gcccagattg gtggcggaga
     1981 aaagatggga gcggaacaca ggcaccccgt ttgtccatag agtgtggcag atggcggcgg
     2041 cgaggccttg gatgttgttc gcggcgatgc cgagaatgat ggaggctccg tcatgcagca
     2101 tgcgcagaag cggttcggtc tcgcgcttct ggtgcagtgc ggcgaggtgg ccgctgaagc
     2161 gggatgtgtg gcgcgcgtag gctccgccaa agaaggacac gcccaggtcc ctcatcacct
     2221 cggcgtcctc ctccggcatg ccccccgcga gcatttgatc aaggatgccg agcggattgc
     2281 ccggcgggtg gaagcgctcc caagtcatct tgtaaagctc gcggatggcg aaccagttgc
     2341 cgggatcggt gacattgtcc ttccagcctg cgcggtcggc ccgggtgaag acgaaaagcg
     2401 cccgcagcct gctggtgccg ccgcagacgg aggccagccg gtccaccttg gatttgtcat
     2461 tgacgccgtc ggccaccagg tcctccagca gacggcggtg ggtgatgagg aagcgcgtca
     2521 gttccagtgt ctccgggaga aatcccgcgt tggagaagca gcgctccagg taatcctcga
     2581 ctggaacgcc tgagaagccg ctggagtatc gctcgccaag gctgaggcgg gtgtcggcgc
     2641 ggcggcgcgc gtcatcggcc atgaggggaa ggcgtttgat tttgagcgcc agcttgacgg
     2701 ccgccatggt gtcgggcgcg gcgcgcgccg tctccacttc atcgagcagg ttgatgtcca
     2761 ccggaaggcc ctccgcgcgt gtctcggaca ggtcgaggcc ggcggcgcgg cgtttttcaa
     2821 ggctgataaa cttctcgacc agaagcgcgc cgcgaagtgt ctttttctct gtcatcacgc
     2881 gctcgtccac ggagacctcg aaagcggcgt ggccggggaa aaggcgctcc tccgcgccgg
     2941 ggaagcgagc cagatgcgcg agagaactgg cgaggctgcc gcgcggctcg taaaacagcg
     3001 cgccgagctc gggcacgggc accagccagt tctcgatgtc cttgaaggcg ctgtccagct
     3061 cggcggggtc aatcggcagg ctgtggcgct gggcttcaag caggaggtcc agggcggcgc
     3121 ggcttttttc ctccggggag acgcagccgg gcgcggtggt gtgcctcatg ccgcccatgc
     3181 cgtaaacgat gccgcgcccg acctcgcggc cctccagcaa ctcccgcccg atggtcgcgc
     3241 gggcgaagcg tgtgacgcgc cggcgcgcgt tgagcagggc ggcgcgcgcg tgcgcggcga
     3301 attcgaaacg gtcgcgctcc gcagcgtccg gtccgagaag atccccgaag gccaggaagt
     3361 cttcaaaacg catcctgtcc tcgatgtgaa ggccgttcgg cagaggctcc atgcgggtgc
     3421 cgcgccgcag ttgcacaaag gcgcgcacac gcagcagcag gtggtaggcg gccatgtcct
     3481 ccggtggcag gatggcgtga acctcgtggc tgtggcggaa gcgccgcccg cccagagtcc
     3541 agacgccggc ctggaaatcc cgcagggcct ccgctttgat gtcgaatcca tccagtttcc
     3601 cggcgtggtg cgcatcctcg tgcatgccgc cgcgggcgcg ccagaattcg ctgatgtgca
     3661 ggaaatgcgc gaaggcgtca tggttcacgc gcaaccgctc gcggaacctg ccggtgaggt
     3721 cgtggggatc gaagacggga ctcagatcaa gcagcgcggt gacggctttg gcgtcgagat
     3781 cggccacata gtcgagctga taggtggcag catggaaggt gaagccgtgc tcgtcaagga
     3841 actcgcgcga gccgttcgtc tgccggtgca tctcgcacag gaagacgctc tggtccggcg
     3901 cttcatcgca gagaaaagcc acgtcaaggt ccgagcaggg cgtcatttca tggcggccgg
     3961 tgccgccgat ggcggcaacg gcgaaggggc gcccgcagcc gcgctccgcc cactcgcgcg
     4021 cggcccatgc ggcaatggcg tcgagatgga tggcggtgcg ccgcgcggtg atctcgcgtc
     4081 cgttgtccag gctgggtccg agaatgagcg cgtcattttc cgcgatgagg cggcggtggc
     4141 gggccagggg agcttcaggg gcattcatgg ccgtggtcaa tagccgctga gcgcgcggtg
     4201 cgtttcacga agcttttgca gacgcgcggc gcgctccagc gagtccggct gccagtcaaa
     4261 gggcgcgagc ttgtgcccgc cattccggac aacggtgacg ccgctggctc ccgtccccat
     4321 gatggcctgc cacgctgtgc cggcgaactc tgccgccagg atgccgtcaa aggccgcggg
     4381 gctgccgccg cgctgcacat agccgagcac ggtggcgcgg gtctcgatct ccttgaaagg
     4441 cccgtgtttg aagcattccc ccaggcgcat ggccaccatc caggcggggc tttcccattt
     4501 gtgcctgtcc attcgcacgc cctcggccac gagcacgatg ctgtgggtgc ggccgcgcgc
     4561 gtgggattgc tcgatctccc cggccagttt ccgaatgcgg tcgccgctga ggaagccgtc
     4621 ctccgggatg atggcgtact ccgcgccgga ggcgatggcg gagagcaagg ccagctcgcc
     4681 gcagccgcgc cccatggttt cgagcaccat gacgcggcga tgactgcgcg cggtgtcttt
     4741 gatcttggtg acctcctgca ggatgttttc gatggcgctc tgcactccaa gcgcccagtc
     4801 ggtgacgggc aggtcattgt ctatggtgcc gggcacgccc acgacctgga tgccgctctc
     4861 ctcatgcagg cggcgcgcgc ccgcgagcga gccgtcgccg ccgcagacaa tgacggcgcg
     4921 gaagccgaga ccgcgcagca tctggatgac acgcgcgcgg atgccgtcgt cgcggaattc
     4981 gtcacagcgc gcgctgcgca gcttgatgcc gccgcgcacg acgagtccgc tgacggattc
     5041 cgggtcgaga tgcaccaagt gctggttttt ggcgtggaaa ccgaagatgc ccggccggtg
     5101 gagtatctcc tcccgcagcg agtccagagt cacttccccg gactccagcc gcgccagggt
     5161 gcgcacgagg ccggcatagc cttccatgac gccgatgacg ggcacgtcgt ggagattgcc
     5221 gccgaggcgc gcggcggcgc gcatgaaggg gttcattccg ggggagtctc cgccactgaa
     5281 ggcgatgagc acgggacgtt tttcaaagag agggttcatt ggagttgaaa gggggttcgg
     5341 atgcggggct ggcgggcggg gtggtgtcgg atggctggcg gaaggatgtg tccgcagcgt
     5401 ccgcgcaagg cggcggggga cttccctcct gttctgaagc aggcgggaca agcgcggggg
     5461 gagtttgggc cgccgcacgc gcaggtttcc ttttgaggat gcggcggcgt tcgcttttgt
     5521 taatcactcc gccgca
//
LOCUS       DAOMYJ010000287         5508 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Prosthecobacter sp. isolate AMR_MDS_5629
            NODE_5076_length_5508_cov_19.921144, whole genome shotgun sequence.
ACCESSION   DAOMYJ010000287 DAOMYJ010000000
VERSION     DAOMYJ010000287.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867850
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prosthecobacter sp. (wastewater metagenome)
  ORGANISM  Prosthecobacter sp.
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Verrucomicrobiaceae; Prosthecobacter.
REFERENCE   1  (bases 1 to 5508)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5508)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.30
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:34:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,742
            CDSs (total)                      :: 4,684
            Genes (coding)                    :: 4,676
            CDSs (with protein)               :: 4,676
            Genes (RNA)                       :: 58
            tRNAs                             :: 55
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5508
                     /organism="Prosthecobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5076_length_5508_cov_19.921144"
                     /isolate="AMR_MDS_5629"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1965333"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..190
                     /locus_tag="PLP58_20120"
     CDS             <1..190
                     /locus_tag="PLP58_20120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ10899.1"
                     /translation="GLEALARRHGMTPSRLRVFFSRTLKRRARCAFDEMAMNDSPGIR
                     EEELDHLWHMLRRYGDAV"
     gene            complement(211..1728)
                     /locus_tag="PLP58_20125"
     CDS             complement(211..1728)
                     /locus_tag="PLP58_20125"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase"
                     /protein_id="HRJ10900.1"
                     /translation="MIPRALLLPLLLSLLAPAAAAPPNVVVFLVDDMGVMDTSVPFLT
                     SGSGEPRRHPLNDYYRTPAMERLAARGIRFNNFYAMSVCSPTRISIMTGQNAARHRAT
                     NWINPQRDNAGPQGPPDWNWRGLKKGDVTLAGLLRGAGWRTIHVGKGHFGPDGAEGAE
                     PLNLGFEVNVAGASFGAPGSYYASLDFGAKTRRAHHAVPGLEKYHGSGTFLTEALTLE
                     ARDRVSRAVADKKPFFLHLAHYAVHGPFDSDPRFAKNYAASGKPAPAQAFATLVEGMD
                     KSLGDLLDHLETLGVAENTLVFFLGDNGTDAPLGHQHEVACAAPLRGKKGAHYEGGMR
                     VPFIASWARPDPANEHQKRLPIPAGVIQAQQASVQDILPTVLALCQITPPKEHQVDGQ
                     DMSALLAGKADASRPEKFLMHYPHAPHRSDYWTSLREGDWKVVYHYFPTEVSGGSHYQ
                     LFNLRDDPFEQKDLAAEDPATLRRMMRALIAELESYAAVYPVAKDGASPVRPVPP"
     gene            complement(1807..2970)
                     /locus_tag="PLP58_20130"
     CDS             complement(1807..2970)
                     /locus_tag="PLP58_20130"
                     /inference="COORDINATES: protein motif:HMM:NF012872.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite reductase subunit alpha"
                     /protein_id="HRJ10901.1"
                     /translation="MSTPAPGKPVYNVKNPYIARVKRAFDLSGPGAPKNTRHYEIDLA
                     GSGLEYTPGDSLAVQPTNDPALVDEMLQVLGFSGAEIVTHPKRPGEQVPVRQALIEAS
                     LVTEVDNKLIKAIIEKTGGQTALNGLLEPEKKEELKSYLWGRFVIDLLIENPAARFEP
                     GEFVGLLKKLNIRLYSISSSQRAVPDEVHLTVATVRYTSHGRERGGVASTFLAERVTP
                     DTPIPVFVNHGKGFRLPEPHEDTPVIFIGPGTGIAPFRAFLQERQATAAKGKAWLFFG
                     EVSQSTCFFYKDEFEAWLAGGTLDRLTTAWSRDQAEKIYVQHKMLEHGREIHEWLEQG
                     AIVYVCGDAARMAVDVDKALHSIVEKEGGKSPEEAAAYMAALKEAKRYRRDVY"
     gene            complement(3084..4775)
                     /locus_tag="PLP58_20135"
     CDS             complement(3084..4775)
                     /locus_tag="PLP58_20135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018969375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HRJ10902.1"
                     /translation="MSRPLALIVYLTMAAFFGCFFAWPIWTTVRLAFEAPDHSFTLEF
                     VGEVFMNPLYREGLLNSFSIAVWTTLGCLVLSVPLALLFVRYDFTGKTLLNSLVLTPM
                     VLPPFVGAIGIKAILGQAGALNALLMNLGLMSRSQPVDWLGEGQMTGIVIMEVLHLYP
                     ILYLNVAAALANLDPAMEEAAANLGCPPWRRFWKVTLPLIMPGMFAGGTLVFIWAFTE
                     LGVPLVFDFDRVTSVQIFRSLNDLSDNPFPYALVVVLLLFSTLIYGSSKLLFGGANAV
                     GGGRATTAREVIRLRFLPGLGCAAVFALVTFLAVVPHLGVALLSVADDWYGTVLPRVL
                     TLDHFHDALGHELTLGSIANSLKYSVAAIFIALLLGIGVAYINVRTRLWGRQLLDVMA
                     MLPLAVPGVVMAFGYLAMTRPGREFDWLIPGENPLLILIIAYAVRRLPYIVRSASAGF
                     QQVSPSLEEAAQNLGARPERALWRVTLPLVAPNLLAGGLLAFAFAMLEVSDSMILAQQ
                     AVHYPITKAIYSLVMALGNGPNLASALGVWAMIFLTVTIAGASLVLGKKLGALFR"
     gene            complement(4869..>5508)
                     /locus_tag="PLP58_20140"
     CDS             complement(4869..>5508)
                     /locus_tag="PLP58_20140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009961053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HRJ10903.1"
                     /translation="GGGVLMNQCPHNLDLFQWMFGMPKSVRGFCQFGRYHQVEVEDNV
                     TAFMAWENGATATFVTSTGEAPGANRLEIAAEQGRLTVTDGTKIHFQRNRQPMSKFCM
                     EAEAAFAMPESWSMEIPVEQSGGQHVEILQNFTNAILKGEKLLSPAEQGICSVELANA
                     ILLSTWQDKTIELPMSSADYERILIEKGETSTFQKTKVVAKASADDFARSFR"
BASE COUNT          905 a         1649 c         2037 g          917 t
ORIGIN      
        1 gggcctggag gcgctggcgc ggcggcacgg catgacgccc tcgcggctga gggtgttttt
       61 cagcaggacg ctgaagcgcc gggcgcggtg cgcgttcgat gaaatggcga tgaacgactc
      121 gccgggcatc cgggaggagg agctggacca tctctggcac atgctgcgcc gctacggaga
      181 cgcggtgtga gcgcgcggcg cggcggggtg tcagggcggc acgggccgga ccggtgatgc
      241 gccgtccttt gccacgggat agacggcggc gtagctctcc agttcggcga tgagggcgcg
      301 catcatgcgg cgcagggtgg cgggatcttc ggcggccagg tctttttgct cgaaggggtc
      361 gtccctgagg ttgaagagct ggtagtgcga tccgccggac acttcggtgg ggaagtagtg
      421 gtagacgacc ttccagtcgc cctcgcgcag gctggtccag tagtcgctgc ggtgcggggc
      481 gtgcgggtag tgcatgagga atttttcggg acgcgaggca tcggccttgc ccgcgagcag
      541 cgcgctcatg tcctggccgt ccacctggtg ctccttcggc ggcgtgatct ggcagagggc
      601 gagcacggtg ggcaggatgt cctgcacgga ggcttgctga gcctggatga ctcctgcggg
      661 gatgggcagg cgcttttgat gctcgttggc ggggtcgggc ctggcccatg aggcgatgaa
      721 gggcactctc atgccgccct cgtaatgcgc gccttttttg ccgcgcagcg gagcggcgca
      781 ggcgacctcg tgctggtggc cgagcggcgc gtcggtgccg ttgtcaccga ggaaaaagac
      841 cagcgtgttc tcagccacgc cgagggtttc gaggtggtcg agcaggtcgc cgagggattt
      901 gtccatgccc tcgacgaggg tggcgaaggc ctgggcgggc gcgggcttgc cgctggcggc
      961 gtagtttttg gcgaagcgcg ggtcggagtc gaaggggccg tgaaccgcgt agtgggcgag
     1021 gtggaggaag aagggctttt tgtccgccac ggcgcggctg acgcggtccc tggcctcgag
     1081 ggtgagggct tcggtgagga aggtgccgga gccgtggtat ttctccagtc cgggcacggc
     1141 gtggtgcgcg cgccgggtct tcgcgccaaa gtcgaggctg gcgtaatagc tgccgggcgc
     1201 gccgaaggag gcgccggcga cgttgacctc gaagccgagg ttcagcggct cggccccctc
     1261 ggcgccgtcg gggccgaagt ggcccttgcc gacatggatg gtgcgccagc cggcgccgcg
     1321 caacaggccg gcgagggtga catcgccctt cttcaagccg cgccagttcc agtccggggg
     1381 gccctggggg ccggcgttgt cgcgctgggg gttgatccag ttcgtggcgc ggtggcgggc
     1441 ggcgttctgg ccggtcatga tggagatgcg cgtgggggag cacacgctca tggcgtagaa
     1501 gttgttgaag cggatgccgc gcgcggccag gcgctccatg gcgggcgtgc ggtagtagtc
     1561 gttgagcggg tggcgcctcg gctcgcccga gccgctggtg aggaatggca ccgaggtgtc
     1621 catgacgccc atgtcatcca ccaggaaaac gacgacattg ggcggcgccg cggcggcggg
     1681 cgcgagcagg gagagcagca gcggcaggag cagggcgcgg gggatcatga tgacgaacgc
     1741 gaacgcgccc ggcgcgcggg tcttgcagcg cgggaaagcg gcccggctcc tggcggcggg
     1801 gcgggctcag taaacgtcgc gccggtagcg cttggcctcc ttgagcgcgg ccatgtaggc
     1861 ggcggcctcc tcgggcgact tgccgccttc tttttcgacg atgctgtgca gcgccttgtc
     1921 cacatccacg gccatgcgcg cggcgtcgcc gcagacatag acgatggcgc cctgctcgag
     1981 ccattcatgg atctccctgc cgtgctcgag catcttgtgc tggacgtaga ttttctccgc
     2041 ctggtcgcgg ctccaggcgg tggtgaggcg gtcgagcgtg ccgccggcca gccaggcttc
     2101 gaactcgtcc ttgtaaaaga agcaggtgct ctggctgacc tcgccgaaaa acagccaggc
     2161 tttgcccttg gcggcggtgg cctggcgctc ctgaaggaag gcgcggaagg gggcgatgcc
     2221 ggtgccgggg ccgatgaaga tgacgggcgt gtcctcgtga ggctcgggca ggcggaagcc
     2281 tttgccgtgg ttcacaaaga cggggatggg agtgtccggc gtgacgcgct cggccaggaa
     2341 ggtggaggcg acgccgccgc gctcgcgccc gtggctggtg tagcgcacgg tggccacggt
     2401 gaggtggacc tcgtcaggca cggcgcgctg gctgctgctg atggagtaaa ggcggatgtt
     2461 gagctttttg agcaggccga cgaactcgcc cggctcgaac ctggcggcgg ggttctcgat
     2521 gaggaggtca atgacgaagc ggccccaaag gtagcttttc agctcctcct ttttctccgg
     2581 ctccaggaga ccgttgagag cggtctggcc gccggttttt tcgatgatgg ccttgatgag
     2641 cttgttgtcc acctcggtga cgaggctggc ctcgatgagg gcctggcgga cgggcacctg
     2701 ctcgccgggg cgcttcgggt gggtgacgat ctccgcgccg ctgaagccga gcacctggag
     2761 catctcatcc acgagcgcgg ggtcgttcgt gggctgcacg gcgagcgagt cgccgggggt
     2821 gtactccagg ccgctgccgg ccaggtctat ctcgtagtgg cgggtgtttt tcggcgctcc
     2881 cggcccggag aggtcaaagg cccgcttcac tctggcgatg tacgggtttt tgacgttgta
     2941 gacgggtttt ccaggggcgg gggtgctcat tgcgatgttt ggtgggatga aaagggggcg
     3001 ggcaggttgg agaaaagccg gggcttgtca attccagcag ccttttccag gaactggaag
     3061 cagatggcgt gccgggtggg gactcagcgg aacaacgcgc cgagcttttt gcccagcacc
     3121 aggctggcgc cggcgatggt gaccgtgagg aagatcatcg cccagacacc gagggccgag
     3181 gcgagattgg ggccgttgcc cagggccatg accagggagt agatggcctt ggtgatgggg
     3241 taatgcaccg cctgctgcgc caggatcatc gagtcgctga cctccagcat ggcgaaggcg
     3301 aaggcgagca gcccgcccgc gagcaggttc ggcgccacca gcggcagcgt gacacgccag
     3361 agagcgcgct cgggcctggc gccgaggttt tgcgccgcct cctccaggct cggactgacc
     3421 tgctggaagc ccgccgaggc ggagcgcacg atgtaaggca gccggcgcac ggcataggcg
     3481 atgatgagga tgagcagcgg attctcgcca ggaatgagcc agtcaaactc gcgccccggc
     3541 cgtgtcatcg ccagataacc gaaggccatc accacacccg gcaccgccag cggcagcatg
     3601 gccatgacat caaggagctg ccgcccccag agccgcgtgc gcacattgat gtaggcgacg
     3661 ccgatgccga gcagcagggc gatgaaaatg gcggcgaccg agtacttgag gctgttggcg
     3721 atggagccga gcgtcagctc gtggccgagg gcgtcgtgga agtggtcgag cgtcagcacc
     3781 cgcggcagca cggtgccgta ccagtcgtcg gccacgctca gcaacgccac tccgagatgc
     3841 ggcaccaccg ccaggaaggt gacgagcgcg aacaccgccg cgcatcccag gccgggcagg
     3901 aagcgcaggc ggatcacctc ccgcgccgtg gtggcgcggc cgccgccgac cgcgttcgcg
     3961 ccgccgaaga gcagcttgct ggagccgtag atcagcgtgc tgaaaagcag cagcaccacc
     4021 accagagcgt aggggaaggg attgtcgctc aggtcgttga gcgagcggaa gatctgcacg
     4081 gaggtgacgc ggtcgaagtc gaagaccagg ggcacgccca gctcggtgaa agcccagatg
     4141 aaaaccagcg tgccgccggc gaacatgccc ggcatgatca gcggcagcgt caccttccaa
     4201 aacctgcgcc acggcgggca gcccaggttg gccgccgcct cctccatcgc cggatccaga
     4261 tttgccaggg cggcggccac attcagatag aggatggggt aaagatgcag cacctccatg
     4321 atgacgatgc ccgtcatctg cccctcaccc agccagtcca ccggctgcga gcggctcatc
     4381 aggccgaggt tcatgagcag cgcgttcagc gcgcccgcct ggccgaggat ggccttgatg
     4441 ccgatggcgc cgacaaaggg cggcagcacc atgggcgtga gcaccaggct gttgagcagc
     4501 gtcttgcccg tgaagtcata gcgcacaaaa agcagcgcca gcggcacgga gagcaccagg
     4561 cagcccagcg tggtccacac cgcgatcgag aaggagttca gcaggccctc ccggtaaagc
     4621 gggttcatga acacctcgcc cacgaattcc agcgtgaagc tgtggtccgg cgcctcgaag
     4681 gccagccgca ccgtcgtcca gatcggccac gcgaagaagc agccgaaaaa ggcggccatc
     4741 gtgagataga cgatgagggc gagcgggcgc gacatgcgcc cggatcatga agacaaaatc
     4801 gaaatccgaa acacgaaatt cgatccgccc gcggtccgcc cccctccggc acggcgcgtc
     4861 gtcacggctc agcggaagct cctcgcgaaa tcgtcggcgc tggccttggc cacgaccttg
     4921 gttttctgga aggtgctcgt ctcgcccttc tcgatgagga tgcgctcgta atcggcgctg
     4981 ctcatcggca gctcgatggt cttgtcctgc caggtggaga gaaggatggc attggccagc
     5041 tcgacgctgc atatgccctg ctcggcgggg ctgagcagtt tttcgccctt caaaatggcg
     5101 ttggtgaagt tttgcaggat ctccacatgc tggccgccgc tttgctcgac ggggatctcc
     5161 atgctccagc tttccggcat ggcaaaggcg gcctccgcct ccatgcagaa cttgctcatc
     5221 ggctggcggt tgcgctggaa gtggatcttc gtgccgtcgg tgacggtgag gcggccctgc
     5281 tcggcggcga tctccagccg gttcgcgccc ggcgcctcgc cggtggaggt gacgaaggtg
     5341 gcggtggcgc cgttttccca ggccatgaag gcggtgacgt tgtcctcgac ctcgacctgg
     5401 tggtagcggc cgaactggca gaagccgcgc acgcttttcg gcatgccgaa catccactgg
     5461 aagaggtcca gattgtgcgg gcactggttc atgagcacgc cgccgccc
//
LOCUS       DAOMYJ010000288         5507 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Prosthecobacter sp. isolate AMR_MDS_5629
            NODE_5077_length_5507_cov_20.252018, whole genome shotgun sequence.
ACCESSION   DAOMYJ010000288 DAOMYJ010000000
VERSION     DAOMYJ010000288.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867850
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prosthecobacter sp. (wastewater metagenome)
  ORGANISM  Prosthecobacter sp.
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Verrucomicrobiaceae; Prosthecobacter.
REFERENCE   1  (bases 1 to 5507)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5507)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.30
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:34:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,742
            CDSs (total)                      :: 4,684
            Genes (coding)                    :: 4,676
            CDSs (with protein)               :: 4,676
            Genes (RNA)                       :: 58
            tRNAs                             :: 55
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5507
                     /organism="Prosthecobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5077_length_5507_cov_20.252018"
                     /isolate="AMR_MDS_5629"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1965333"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1087)
                     /locus_tag="PLP58_20145"
     CDS             complement(<1..1087)
                     /locus_tag="PLP58_20145"
                     /inference="COORDINATES: protein motif:HMM:NF024668.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4062 domain-containing protein"
                     /protein_id="HRJ10904.1"
                     /translation="MQKPPASPRPRVFLSTVSREFKTLRAYIAKVVRHLGYDPVSMDD
                     WSAAHGELRAWLRAQLESCDGIIHLAGLAYGAEPETHDPAAHGLLDDTPRYSYTHYEW
                     HYAQAIGLKSWLILPGADCTRDTAPEALDLPEDPAHPDPAAYQSERRLWQAAHLARLQ
                     AANHVHHHPANDHELHRLLDTLHDHAAEMRERFGQWQGFVTDYLARMEGKVDRVQRDT
                     RHLRKAALLGLAALMLVAGGVSLVWRQARQTGDDIAETRKEISEIKDIHARERKFMAL
                     ILGKANERLKDWESMTPAMRFDLALDEIAADQKIPARELRDMLDLYVARVEADPDAEA
                     EDKFYALMRRQAFGEAATLARAEAGGAE"
     gene            complement(1242..5087)
                     /locus_tag="PLP58_20150"
     CDS             complement(1242..5087)
                     /locus_tag="PLP58_20150"
                     /inference="COORDINATES: protein motif:HMM:NF037660.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HRJ10905.1"
                     /translation="MPDETRATPPAPDKPPASPRPRVFLSTVSREFKTLRMEIEKVVR
                     HLGYDPVSMDDWSAAHGELRAWLRAQLESCDGIIHLAGLAYGAEPETHDPAAHGLLDD
                     TPRYSYTHYEWHYAQAIGLKSWLILPGADCTRDTAPDELDLPVDPAPPGQPPPDPAVH
                     QAARRLWQAEHIARLKAQNHARHEPADDLQLHKLLDTLHDHAAEMRQRFAQWQGFVTE
                     YLARMEGKLDAAAEGVRRVEGQTRGLRKAAALGVAALLVIGAAGWWIRRDTSQTAKGQ
                     DEIVQGQARLEEQMRKMRESLGIIQQQSDPLLDPVADWPRERLERALARQMGMPEEEL
                     RALLAAGRTSVDELLQGQALLASGQSADAVARFDRVISQEQAATERRSLAYLGKSNAA
                     YMQGDRAAALALSGRACALADREENPGLWAELHFNQGFLLWEEGRLAEASKMLQQVLE
                     AREQTEGETSADTLSACFMLARVLQDQGSEQEAFFLYERVLEDPRRLLPAASHIRFYA
                     RYNLAYLLFEAGDAGEAERLARESLASGVEDFGEGSAMHVESLVLLAVILRDKGSLAE
                     ARDLADGAAELAQRHLPVGSPVKEQTLALRASMLQTFGHYQAAEGIYRSLAETQSALE
                     KDGGPKTAAALADLAQAILDRGERTGALEMLARSVAMLEKHHGTDSHVVWTARDNLAL
                     ALAETGDYSGAEALHQQVLAARTEKYGAEHPATLLSLGNLADAQKQQGSYEKAEKNMR
                     AVYQASLRLYGPDHARTLSSLNNLAVLLKEKGDYAAAEPLQRQAYEDSLRHLGKEHPY
                     TLRYLNNLALVLEHKGDYAAAEPLQRQAYEDSLRVLGKDHPDTLNHLNNYAVLLEHKG
                     DLKAAEPLQRQAYEHSRRVLGKDHPLTLSRLNNLAVLLERKGDYAAAEPLQRQAYEDS
                     RRIHGKDHPQTLSSLNNLAVLLEHKGDLAAAETLQRQAYEDTRRILGKDHIETLNRLN
                     NLAVLLERKGDHAAAGPLQRQAYEDCLRVLGKDHPQTLNCLNNLAVFLEHKGDYAAAE
                     PLQRQAYEDSLRVLGRDHIDTLNRLHNLTVLLQNKGDHAAAEPLLRQAHEDSRRILGG
                     DHPQTLRLLDSLAALLCNSGRHLAAEPLQRQAYEGRQRVLGDHHSDTLHSLNNYAGVI
                     LANHGPEKAGPLFQQALDERRRHLGAEHPDTLHSLHDMAKLMRKKGDTARAEELFRQA
                     WQARERVLGADHPRTLASLHDLAEMLEEEGRHAEARELFLRAMDSARRVFAEDHPTRV
                     DYQSHLDRIEKALKGKPNP"
BASE COUNT          776 a         1776 c         1952 g         1003 t
ORIGIN      
        1 gctcggcgcc gccggcctcg gcgcgcgcca gcgtggcggc ctcgccaaaa gcctggcggc
       61 gcatcagcgc ataaaacttg tcctccgcct ccgcgtccgg gtcggcctcc acccgcgcga
      121 cgtagaggtc cagcatgtcg cgcagttccc tggccgggat cttctggtcg gcggcgatct
      181 catcgagcgc caggtcaaag cgcatggcgg gcgtcatgct ctcccagtcc ttgagacgct
      241 cgttggcctt gcccagaatg agggccatga acttgcgttc cctggcgtgg atgtccttga
      301 tctcgctgat ctctttgcgc gtctccgcga tgtcatcccc cgtctgccgc gcctgcctcc
      361 agaccaggga aacgccaccc gccacgagca tgagcgccgc cagccccagc agggcggctt
      421 tgcgcaggtg gcgggtgtcg cgctggacgc ggtccacctt gccctccatg cgcgccaggt
      481 aatccgtcac aaagccctgc cactggccga accgctcgcg catctcggcg gcgtggtcgt
      541 ggagggtgtc gagcaggcgg tggagttcgt gatcgttggc ggggtggtgg tggacgtggt
      601 tggcggcttg caaccgcgcg aggtgcgccg cctgccaaag cctgcgctca ctctgataag
      661 ccgccgggtc gggatgcgcg gggtcttcgg gaaggtcgag agcctcgggg gcggtgtcgc
      721 gcgtgcagtc cgcccccggc aggatgagcc agcttttcag gccgatggcc tgggcgtagt
      781 gccactcgta atgggtgtag gaataacggg gagtgtcatc cagcaggcca tgcgcggcgg
      841 ggtcgtgggt ctcaggctcg gcgccgtagg ccaggccggc gaggtggatg atgccgtcgc
      901 aactctccaa ctgcgcgcgc agccaggcgc gcagctcgcc atgcgcggcg gaccagtcat
      961 ccatgctcac cggatcataa ccgagatggc gcacgacctt ggcgatgtac gcccgcaggg
     1021 tcttgaactc cctgctgacc gtggagagaa aaacgcgcgg gcgcggggag gcgggcggct
     1081 tctgcatggg ctgggatcag gggttgggct tgcccttgag ggccttttcg atgcggtcga
     1141 gatggctctg gtagtccacg cgggtggggt ggtcctccgc gaagacccgg cgcgcggagt
     1201 ccatggcgcg gggaggcggg cggcttctgc atgggctggg atcaggggtt gggcttgccc
     1261 ttgagggcct tttcgatgcg gtcgagatgg ctctggtagt ccacgcgggt ggggtggtcc
     1321 tccgcgaaga cccggcgcgc ggagtccatg gcgcggagaa aaagctccct ggcctcggca
     1381 tgacggccct cttcctccag catctcggcc agatcatgca ggctggccag cgtccgcgga
     1441 tggtcggctc caagcacgcg ctcgcgggcc tgccatgcct ggcggaaaag ctcctccgcc
     1501 cgcgcagtgt cccccttttt ccgcatgagc ttggccatgt catgaaggct gtggagcgtg
     1561 tccgggtgct cggctcccag gtggcgccgg cgctcgtcca atgcctgctg gaaaagcggc
     1621 ccggcttttt cagggccgtg gttggcgagg atgacacccg cgtagttgtt gaggctgtgc
     1681 agggtgtcgg agtggtggtc gccgagcacg cgctggcggc cctcgtaagc ctggcgctgg
     1741 agcggctcgg cggcgaggtg cctgccgctg ttgcaaagca gggccgccag gctgtccagc
     1801 agacgcagtg tctgcggatg gtccccgccc aggatgcgcc ggctatcctc atgggcctgg
     1861 cgcaggagcg gctcggcggc ggcgtggtcg cccttgttct ggagcagcac ggtcaggtta
     1921 tggagccggt tgagagtatc aatgtggtcc ctgcccagga cgcgcagact gtcctcataa
     1981 gcctggcgct ggagcggctc ggcggcggcg tagtcgccct tgtgttcaag aaagacggcc
     2041 aggttgttga ggcagttgag ggtttgtgga tggtccttgc ccagcacgcg cagacagtcc
     2101 tcataagcct ggcgctggag cggcccggcg gcggcatggt cgcctttgcg ttcgagcagc
     2161 acggccaggt tgttgaggcg gttgagtgtt tcaatgtggt ccttgccgag gatgcgccgg
     2221 gtgtcctcat aagcctggcg ctggagcgtc tcggcggcgg cgaggtcgcc cttgtgttcg
     2281 agcagcacgg ccaggttgtt gaggctgctg agggtttgtg gatggtcctt gccgtggatg
     2341 cgccggctgt cctcgtaagc ctggcgctgg agcggttcgg cggcggcgta gtcacccttg
     2401 cgttcaagca ggacggccag gttgttgagg cggctgaggg ttagagggtg gtccttgccc
     2461 agcacgcgcc ggctgtgctc ataggcctgg cgctggagcg gctcggcggc tttgaggtcg
     2521 cccttgtgtt cgagcaggac ggcgtagttg ttgaggtggt tcagagtatc gggatggtcc
     2581 ttgcccagga cgcgcaggct gtcctcataa gcctggcgct ggagcggctc ggcggcggcg
     2641 tagtcgccct tgtgttcgag cacgagggcg aggttgttga ggtatctcag cgtgtaggga
     2701 tgctccttgc ccagatggcg caggctgtcc tcataggcct ggcgctggag cggttcggcg
     2761 gcggcgtagt cgcctttctc tttgagcagg acggcgaggt tgttgaggct gctcagagtg
     2821 cgggcgtggt cggggccgta aaggcgcagg ctggcctggt aaaccgcgcg catgttcttc
     2881 tccgcctttt cataactgcc ctgctgcttt tgcgcgtcgg ccaggttgcc caggctgagc
     2941 agcgtggcgg gatgctcggc cccgtacttc tcggtgcgcg cggccaggac ctgctggtgc
     3001 agggcctccg cgccggagta gtcccccgtt tccgccagag ccagcgcgag attgtcgcgc
     3061 gccgtccaga ccacatggga gtccgtgcca tgatgctttt ccagcatcgc cacggaccgc
     3121 gccagcatct ccagggcccc ggtgcgttcg ccccggtcca ggatggcctg ggcgaggtct
     3181 gcgagcgccg ccgccgtctt cggtccgccg tccttctcca gggcggactg tgtctccgca
     3241 aggctgcggt agatgccctc cgccgcctgg taatgcccga aagtctgcag catggaggcc
     3301 cgcagcgcca gggtttgctc cttcacaggg ctgccaaccg gaaggtgcct ttgcgccagt
     3361 tccgccgccc cgtcggccaa gtccctggcc tcggcgaggc tgcccttgtc ccgcaaaatc
     3421 acggccagca ggacgaggct ttccacatgc atggcggagc cttcgccgaa gtcctcgacg
     3481 ccggaggcca ggctttcgcg cgccagcctc tcggcctccc cggcatcccc cgcctcaaag
     3541 agcagatacg ccagattgta acgggcatag aaccggatgt gggaggccgc cggcagaagc
     3601 ctcctggggt cctccagcac gcgctcgtag aggaaaaaag cctcctgctc actgccctgg
     3661 tcctggagca cgcgcgccag catgaagcag gcgctcagcg tgtcggcgga ggtctcgccc
     3721 tccgtctgct cgcgcgcctc cagcacctgc tgcagcatct tcgaagcctc cgccagacgc
     3781 ccttcctccc aaagcagaaa gccctggttg aaatgcagct ccgcccagag accgggattc
     3841 tcctccctgt ccgccagggc gcaggcgcgg ccggacaagg ccagcgcggc ggccctgtcc
     3901 ccctgcatgt aagcggcgtt ggacttgccc agataggcca ggctgcgcct ctccgtggcc
     3961 gcctgctcct ggctgatcac gcggtcaaac ctcgccaccg cgtcggcgct ctgcccggag
     4021 gccagcagcg cctgcccctg gagcagctcg tccacgctcg tgcgccccgc ggcaagcagc
     4081 gcgcgcagct cctcctccgg catccccatc tgccgcgcca gcgctctttc cagacgctcc
     4141 ctcggccagt cggccacagg gtcgagcaaa gggtcgctct gctgctggat gatgccgaga
     4201 gactcccgca ttttccgcat ctgctcctcc agccgggcct ggccttgcac aatctcgtcc
     4261 tggcccttcg cggtctggct cgtgtccctc ctgatccacc agccggccgc gccgatgacc
     4321 agcagcgccg ccaccccgag cgcggcggct ttgcgcaggc cgcgcgtctg cccctccaca
     4381 cggcggacgc cctccgccgc ggcatcgagc ttcccctcca tgcgcgccag gtactccgtc
     4441 acaaacccct gccactgcgc aaacctctgc cgcatttccg ccgcgtggtc gtgcagcgtg
     4501 tccagcagct tgtgcagttg aagatcatcc gcaggctcgt ggcgggcatg gttttgggct
     4561 ttcagccggg cgatgtgctc cgcctgccag agccggcgcg ccgcctggtg aacggcgggg
     4621 tcggggggag gctggccggg aggcgcgggg tccacgggaa ggtcaagctc gtcgggcgcg
     4681 gtgtcgcgcg tgcagtccgc ccccggcagg atgagccagc ttttcaggcc gatggcctgg
     4741 gcgtagtgcc actcgtaatg ggtgtaggaa taacggggag tgtcatccag caggccatgc
     4801 gcggcggggt cgtgggtctc aggctcggcg ccgtaggcca ggccggcgag gtggatgatg
     4861 ccgtcgcaac tctccaactg cgcgcgcagc caggcgcgca gctcgccatg cgcggcggac
     4921 cagtcatcca tgctcaccgg atcataaccg agatggcgca cgaccttttc gatttccatc
     4981 cgcagggttt tgaactccct gctgacggtg gagagaaaaa cgcgcgggcg cggggaggcg
     5041 ggcggcttgt ccggggcagg gggcgtggcg cgggtctcgt caggcatggg cggcggggga
     5101 gttccggcgg gagaatggcg gcaaagcgcg cggcggggaa gcgggaaatg cgggcggcgg
     5161 tgaaaaaatg cgcggggatg ccgcgccgtc atgccggggt gggaggccgg cgcctggagt
     5221 gatggagtgt tggaatgccg ggtccaggat tccatcagga ctgcacggct cctggaaccg
     5281 ccaaagggga ctcccgaaaa ctcagggcat ggggcgcggg catcctgcct gcgcggacag
     5341 gcgggacgcc tgttccccat gcaaaggcgg gttttcggga gtgcctaaaa tttgccggcg
     5401 cggggtcttt tttccaacgc ctcgcctctt ttcctgtcat gcatggcagc gatgccccac
     5461 accccgaccc gccatgaaaa cgccacttca ggttcgacgg tgatttc
//
LOCUS       DAOMYJ010000289         5422 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Prosthecobacter sp. isolate AMR_MDS_5629
            NODE_5194_length_5422_cov_18.919694, whole genome shotgun sequence.
ACCESSION   DAOMYJ010000289 DAOMYJ010000000
VERSION     DAOMYJ010000289.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867850
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prosthecobacter sp. (wastewater metagenome)
  ORGANISM  Prosthecobacter sp.
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Verrucomicrobiaceae; Prosthecobacter.
REFERENCE   1  (bases 1 to 5422)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5422)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.30
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:34:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,742
            CDSs (total)                      :: 4,684
            Genes (coding)                    :: 4,676
            CDSs (with protein)               :: 4,676
            Genes (RNA)                       :: 58
            tRNAs                             :: 55
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5422
                     /organism="Prosthecobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5194_length_5422_cov_18.919694"
                     /isolate="AMR_MDS_5629"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1965333"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..683
                     /locus_tag="PLP58_20155"
     CDS             <1..683
                     /locus_tag="PLP58_20155"
                     /inference="COORDINATES: protein motif:HMM:NF012257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030551 - cyclic nucleotide binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HRJ10906.1"
                     /translation="LRAVVMANTVSLKLLADLCGRMRRLGVQLVVCQPGGLAPRLQSA
                     GVPADCLFSTLDAALEKCEDALLLAVTGVSWRPARGLKLAECPVFQSCSPEEARMLEQ
                     TLTRRTFQTDETLIKAGESSDEMFVLLEGNVEVRIKGKLGLSQRVDVLTSGMTAGEMA
                     FLDGSPRSADVVAMDRVECLVMTRAWFESLSETHPELKIRLLQAFMREISSRLRQANI
                     AIAALHSQ"
     gene            complement(770..1903)
                     /gene="murG"
                     /locus_tag="PLP58_20160"
     CDS             complement(770..1903)
                     /gene="murG"
                     /locus_tag="PLP58_20160"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: protein motif:HMM:TIGR01133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050511 -
                     undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HRJ10907.1"
                     /translation="MKPSSKKPAHVLIACGGTGGHLFPGIAVGEVLAARGHPVTLFIS
                     EKKIDAIAASGHKELRFEKMPFLAMPKPWSPKMPGFLLGVWRGIQASRKFIRDNDVAL
                     VLGMGGFTSFAPLYAGRKEGRHTLIHESNAIPGKANRLNARYAGTVLCGLGACKSAFA
                     AHPDVRVTGTPVRSSMKTASKEDPHAFFKLDKNKKTLLVMGGSQGARGINRVVGMTLE
                     HFEKMDVQVLHIAGPGDYEEVRDTYARHPMLSQHVAAFCHRMDLAWRVADLALARSGA
                     STMAEIALFGVPSLLVPYPFAADDHQTRNAEVFANAGAALLLPESGLNAEVLADAARS
                     ILQDGRKAAEMRRAAGSLAVRDAAQKIATLIEKTLKNQTGAKR"
     gene            complement(1910..3088)
                     /locus_tag="PLP58_20165"
     CDS             complement(1910..3088)
                     /locus_tag="PLP58_20165"
                     /inference="COORDINATES: protein motif:HMM:NF013279.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative peptidoglycan glycosyltransferase FtsW"
                     /protein_id="HRJ10908.1"
                     /translation="MARRSVFILLAAVFLLTGLGITMLASTSVYSVKAGEDYVTVWKQ
                     CVSAGVALLAGLIAALVDYHVWHRLRWWIYGLACAGLALCFEWHLAKLAGYEPLIGVW
                     KGGAARWLKTGLGDMQLQPSEFAKIALIIALAGWFVAHEGKERTFRHGLLLPGLILGG
                     VTGLIGLEVDLGNGSLAIAVGMGILFVAGTRARYLTILGGLGGALLAAGIWLAPKRFE
                     RVIAIFDLEKYKDGVGLQQWISWLAFGSGGLDGRGLGEGRMKHSYLPEAHTDFIFPMI
                     GEEIGLWGSLGTLLIFVVIALCGMSIAMHAPDRFGRLLGFGLTLLISLEALLNMGVTT
                     ALLPNKGLPMPFVSYGGSSLVAAMVAVGILVNIHCRSTHSARDQLPLIRRDKRWTPQI
                     "
     gene            complement(3101..4159)
                     /locus_tag="PLP58_20170"
     CDS             complement(3101..4159)
                     /locus_tag="PLP58_20170"
                     /inference="COORDINATES: protein motif:HMM:NF013630.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HRJ10909.1"
                     /translation="MSTTRQKKMLLPDSPRRRKIKQHQMIVATVTEEEGFNPPVPHTD
                     TLRLLVAIFLIHAVVIGAIIGYDRMSGVTEETRTMKKDTRGEAVPSVLPPSAVNTDLL
                     QKEYPIEEYATYEWRSGDSLPKVAEKLRVPQDLLVRLNMLDKGAQIATNTILRYPRQP
                     AVKAVGIGVAGADGLPPVTAVAFEPPPPAGSLAASSPSIGLEMPGEAPFSLSPNIMHE
                     LAQGPDLGSGAVSAPDSPPPAQPVAEKTPAVQDQPPPAPPPVVKTAGEKPVPKAIPVP
                     RSYTPPAPPAARKSAPPPAVKPAPQRQAASHVVRPGENLYRIAARYKVDVKSIQDANK
                     LKNPDHIREGMKLVIPAK"
     gene            complement(4181..>5422)
                     /gene="murD"
                     /locus_tag="PLP58_20175"
     CDS             complement(4181..>5422)
                     /gene="murD"
                     /locus_tag="PLP58_20175"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR01087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008764 -
                     UDP-N-acetylmuramoylalanine-D-glutamate ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HRJ10910.1"
                     /translation="ARVTVMDEGDPARIAPALEKLHAEGFDTLPGAPARDLVVRPGEW
                     SRAVISPGLDAGWPLPKKFTSAGVPLTGEMEFGYGFCRTSLAAITGTNGKSTCTEITA
                     AVFNGCGLKSLPCGNHGVSLSEVVASGENYDVLALEVSSFQLETIRAFRPRVAVWLNF
                     APDHLDRYPGMEEYFSAKARIFENMTGEDLAVVRAGEPVNSGAARRVTFSAHGMEADW
                     TWADGVIRHPGGEAPTGGIRLRGRHNMENLLAALIACQSFGLDVPDMLRAAAGFEPPA
                     HRCELVREWRGREFINDSKATNLHALNACLLAMDRPVILIAGGKDKQLDYAPLLASLP
                     GRVRAMVLIGEIAPALHSLFSPHLPCERAADMDEAVRRAARLSQEGDLVLLSPGTSSF
                     DMYTGYAQRGEAFRAAALSLH"
BASE COUNT          904 a         1627 c         1951 g          940 t
ORIGIN      
        1 acctgcgcgc ggtggtgatg gcgaacacgg tgagcctgaa actgctggcg gacctgtgcg
       61 ggcggatgcg ccggctgggg gtgcaactgg tggtgtgcca gccgggcggc ctggccccgc
      121 gcctgcaaag cgcgggcgtg ccggcggact gcttgttttc caccctggac gcggcgctgg
      181 agaaatgcga ggacgcgctg ctcctggctg tgacgggggt gtcatggagg ccagcgcgcg
      241 gtctgaagct ggcggaatgc cccgtcttcc agtcctgctc gccggaggag gcgcggatgc
      301 tggagcagac gctgacgagg cggaccttcc agacggacga gacgctgatc aaggcgggcg
      361 agagctccga cgagatgttt gtgctgctgg aggggaatgt ggaggtgcgg atcaagggca
      421 agctggggct gagccagcgg gtggacgtgc tgacctccgg catgacggcg ggtgagatgg
      481 ccttcctcga cggctcgccg cgctcggcgg atgtggtggc gatggaccgg gtggagtgcc
      541 tggtgatgac gagggcatgg ttcgagtcgc tgtcggagac gcaccccgaa ctgaagatca
      601 ggctgctcca ggcgttcatg agggagatct catcccgcct gcggcaggca aacatcgcca
      661 tcgcggcgct gcacagccaa tgaggcgggc ggggctgaat ccgtgcccgt ccgtgtgatt
      721 cgtgtttttt tcccgctccc aagttgaccc gttggcggcg gcgcgcttct cagcgtttgg
      781 cgccggtctg gtttttgagg gtcttttcga tgagggtggc gattttttgc gcggcgtcgc
      841 gcacggcgag actgcctgcg gcgcggcgca tttcggcggc tttgcgcccg tcctggagga
      901 tggagcgcgc ggcgtcggcg aggacttcgg cattgaggcc ggactcgggc agcagcaatg
      961 cggcgccggc attggcgaag acttcggcgt tgcgcgtctg gtggtcatcc gcggcgaagg
     1021 ggtagggcac aagcaggctg ggcacgccaa agagggcgat ctcggccatg gtggaggcgc
     1081 cgctgcgggc gagggcgaga tcggcgacgc gccaggcgag gtccatgcgg tggcagaagg
     1141 cggcgacatg ctgggagagc atggggtggc gggcataggt gtcgcgcact tcctcatagt
     1201 cgccgggacc ggcgatgtgg aggacctgga catccatttt ttcaaagtgc tcgagggtca
     1261 tgccgacgac tcggttgatg ccgcgcgcgc cctggcttcc gcccatgacg aggagggttt
     1321 ttttgttttt gtcgagcttg aagaaggcgt gggggtcctc cttggaggcg gtcttcatgc
     1381 tggagcgcac gggggtgccg gtgacacgca cgtcggggtg cgcggcgaag gcgcttttgc
     1441 acgcgccgag gccgcagagg acggtgcctg cgtagcgggc gttgagtcgg ttggccttgc
     1501 cggggatggc gttgctctcg tggatgaggg tgtggcggcc ttccttgcgc ccggcataga
     1561 gcggggcgaa ggaggtgaag ccgcccatgc cgagaacgag ggccacgtcg ttgtcgcgga
     1621 tgaatttccg cgaggcctga atgccgcgcc agactccgag caggaagccg ggcattttgg
     1681 gcgaccacgg cttcggcatg gccaggaagg gcattttttc gaaccgcagc tccttgtgac
     1741 cggaggcggc gatggcgtct atttttttct cgctgatgaa aagggtgaca gggtgcccgc
     1801 gcgcggcgag cacctcaccg acggcgatgc cggggaacag gtgcccgccg gtgccgccgc
     1861 aggcgatgag cacatgggcg ggctttttcg aggatggttt cacggaaaat caaatctggg
     1921 gcgtccagcg cttgtccctg cggatcagcg ggagctggtc acgcgcggaa tgggtgctgc
     1981 gacagtgaat gttgacaagg atgcccaccg ccaccatggc ggccacaagg ctggagcccc
     2041 cgtacgagac gaaaggcatg ggcaggccct tgttcggcag caaagcagtg gtgacgccca
     2101 tgttcaagag cgcttccaga ctgatgagca gggtgagacc aaagcccagc aggcggccga
     2161 agcggtccgg cgcgtgcatg gcgatggaca tgccgcagag ggcgatgacg acgaagatca
     2221 gcagggtgcc caggctgccc caaaggccga tctcctcccc gatcatggga aaaatgaagt
     2281 cggtgtgcgc ctcgggcagg taggagtgct tcatgcgccc ctcgccaagc ccgcgcccgt
     2341 ccaggccgcc ggagccgaag gcgagccagg agatccactg ctggagaccg actccgtcct
     2401 tgtacttctc caggtcaaag atggcgatga cgcgctcgaa ccgcttcggc gccagccaga
     2461 tgcccgccgc cagcagcgcg ccacccaatc cgccaaggat ggtcaaatag cgcgcgcggg
     2521 tgcccgccac aaagaggatg cccatgccga cggcgatggc gaggctgcca ttgccaaggt
     2581 caacctccag tccgatcaag ccagtgacgc cgccaaggat gagaccgggc agcagcaggc
     2641 cgtgcctgaa tgtgcgctcc tttccctcat gcgcgacgaa ccagcccgcg agggcgatga
     2701 tgagggcgat ttttgcaaac tccgaaggct gcaactgcat gtcgcccagg cccgttttca
     2761 accagcgcgc ggcaccgcct ttccagacgc cgatcaacgg ctcatagccg gcgagctttg
     2821 ccaggtgcca ttcgaaacaa agcgccagcc ccgcgcaggc gagtccgtaa atccaccagc
     2881 gcagccggtg ccagacgtgg taatccacca gggccgcgat cagccccgcc agcaaggcca
     2941 caccggcgga gacgcactgc ttccagacgg tgacgtagtc ctcgccggcc ttcacggaat
     3001 aaacactggt gctggccagc atggtgatgc ccagccccgt gagcagaaac acggccgcca
     3061 gcagaatgaa aacggagcgt ctggccatct gagtgctttc ttatttggcg ggaatgacca
     3121 gcttcatgcc ttcgcggatg tggtccggat ttttgagctt gttggcgtcc tggatggatt
     3181 tcacatccac cttgtaccga gcggcgatgc ggtaaagatt ctcacccggc ctgacaacat
     3241 gggatgccgc ctgcctttgg ggtgcgggct tgaccgccgg cggcggcgcg gatttgcgcg
     3301 cggcgggcgg tgcgggcggt gtgtaggaac gcggtaccgg tatggccttg ggcacgggct
     3361 tttccccggc cgttttcacc acaggcggag gcgcgggcgg cggctggtcc tggactgccg
     3421 gggttttttc cgcgacgggc tgcgccggcg gcggcgaatc tggagccgac accgcgccgg
     3481 agccgagatc gggcccctgg gccagctcat gcatgatgtt gggagagagg ctgaaaggcg
     3541 cctcaccagg catttcgaga ccgatggaag gcgaggacgc ggccaggctg cccgcgggcg
     3601 gcggcggctc aaatgccacg gcggtcaccg gcggcaggcc gtccgctcca gcaacaccaa
     3661 taccgacggc cttgacggcg ggctgcctgg gataacgcag aatggtgttc gtggcgatct
     3721 gcgccccctt gtccagcatg ttcaggcgca cgagcaggtc ctgcggcacg cgcagttttt
     3781 ccgccacctt gggcagcgag tcgccggagc gccattcata agtggcgtat tcttcgatgg
     3841 gatactcctt ttgcagcaga tcggtgttga ccgcggaggg cggcagcacg ctcgggacgg
     3901 cctcgccacg ggtgtctttt ttcatcgtgc gtgtctcctc ggtcacgccg ctcatgcggt
     3961 cgtagccgat gatggcgccg atgacgacgg cgtgaatgag gaagatggcg acaagcagtc
     4021 tcagcgtgtc ggtgtggggc acgggcgggt tgaagccctc ctcctcggtg acggtggcca
     4081 cgatcatctg gtgctgtttg attttccgcc gcctggggga atccggcagc agcattttct
     4141 tttgccttgt ggtgctcatg gtgttcggga gttggcgggt tcaatggagt gaaagtgcgg
     4201 cggcgcggaa ggcctccccg cgctgtgcgt agccggtgta catgtcgaag ctggaggtgc
     4261 ccggactgag gaggacgagg tcgccctcct gggagaggcg cgcggcgcgc cgcacggcct
     4321 cgtccatgtc cgcggcgcgc tcgcagggca gatgcgggga aaatagcgag tgcagggcgg
     4381 gagcgatctc gccgatgagg accatggcgc gcacgcggcc gggaaggctg gccagcagcg
     4441 gcgcgtagtc gagctgcttg tccttgccgc cggcgatgag gatcacgggc cggtccatgg
     4501 cgagcaggca tgcattgagg gcatggagat tggtggcctt cgagtcgttg atgaactccc
     4561 gcccgcgcca ttcgcgcacc agctcgcaac gatgcgccgg aggctcaaac ccggcggcgg
     4621 cgcgcagcat gtccggcacg tccaaaccaa agctctgaca ggcgatgagc gcggccagga
     4681 ggttctccat gttgtgccgt ccgcgcaggc ggatgccgcc ggttggcgcc tcgccgccgg
     4741 gatgccggat gacgccgtcc gcccatgtcc agtccgcctc catgccatgc gccgaaaagg
     4801 tgacacggcg cgccgcgccg ctgttgaccg gctctccggc gcgcaccacg gccaggtctt
     4861 ccccggtcat gttttcaaaa atgcgcgcct tggcggaaaa gtactcctcc atgcccggat
     4921 agcggtccag atggtcgggc gcgaagttca accacacggc cacgcgcgga cggaaggcgc
     4981 gaatggtctc gagctggaag ctgctgacct ccagggcgag cacgtcgtag ttttcccccg
     5041 aggccacgac ctcgctgaga ctgacgccgt gattgccgca ggggagggat ttcagaccgc
     5101 agccattgaa gacggcggcg gtgatctccg tgcatgtgct cttgccattg gtgccggtga
     5161 tggccgccag ggacgtccgg caaaagccgt agccgaactc catttcacct gtgagcggca
     5221 ctccggcact ggtgaatttt tttggcaaag gccagcccgc gtcgagtccg gggctgatga
     5281 ccgcccggct ccattccccc ggcctcacca ccaggtcgcg cgccggcgcg cccgggagcg
     5341 tgtcaaaacc ctcggcgtga agtttctcca acgccggcgc gatgcgcgcg gggtcgccct
     5401 cgtccatgac cgtcacccgg gc
//
LOCUS       DAOMYJ010000290         5420 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Prosthecobacter sp. isolate AMR_MDS_5629
            NODE_5195_length_5420_cov_20.761603, whole genome shotgun sequence.
ACCESSION   DAOMYJ010000290 DAOMYJ010000000
VERSION     DAOMYJ010000290.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867850
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prosthecobacter sp. (wastewater metagenome)
  ORGANISM  Prosthecobacter sp.
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Verrucomicrobiaceae; Prosthecobacter.
REFERENCE   1  (bases 1 to 5420)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5420)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.30
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:34:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,742
            CDSs (total)                      :: 4,684
            Genes (coding)                    :: 4,676
            CDSs (with protein)               :: 4,676
            Genes (RNA)                       :: 58
            tRNAs                             :: 55
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5420
                     /organism="Prosthecobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5195_length_5420_cov_20.761603"
                     /isolate="AMR_MDS_5629"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1965333"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..207)
                     /locus_tag="PLP58_20180"
     CDS             complement(<1..207)
                     /locus_tag="PLP58_20180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ10911.1"
                     /translation="MTRHATPSAAHAAGFSLVETIMAMGITSTALLAVMGMLSTTLGD
                     ARESRVETAAGILTRRIVAEARGLP"
     gene            complement(204..965)
                     /locus_tag="PLP58_20185"
     CDS             complement(204..965)
                     /locus_tag="PLP58_20185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ10912.1"
                     /translation="MSVRRAGTGVHFAPATRPALMRRPRHTSSFPPPGRSHVRHAPGY
                     TMIEMMFVVIVITLVVALTAGVTDSLKSNRGTTAMQQLVSVMDSGRAKALAGQQEIWI
                     AFANGEARAPAQAFRSYAVCYSIEDDNLPEGQRSILLPLTGWEHLPTGFVFTLTAPAL
                     QSAGQNLMSHPAAAKRVRFSASNPAGMGNLPCIRFGTLGEVEQPESGGTLLLAVAEGE
                     VRGSIPVKMSGGGHDPAYCRWLSVQRNTGKAVLLP"
     gene            1113..1586
                     /gene="bcp"
                     /locus_tag="PLP58_20190"
     CDS             1113..1586
                     /gene="bcp"
                     /locus_tag="PLP58_20190"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007415812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-dependent thiol peroxidase"
                     /protein_id="HRJ10913.1"
                     /translation="MLSRPEPGKKAPAFSAPAAGGGLKGGAKVTLKDFQGRPLVLYFY
                     PKDDTPGCTRQACALRDGWARISEKAQVLGVSGDSIASHEKFIKKHSLPFPILSDEDH
                     AVASAYGVWVEKSMYGKKHMGIERSTFVIGPGGVISAVFPKVKPDEHLERVLAAL"
     gene            complement(1638..4889)
                     /locus_tag="PLP58_20195"
     CDS             complement(1638..4889)
                     /locus_tag="PLP58_20195"
                     /inference="COORDINATES: protein motif:HMM:NF013069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HRJ10914.1"
                     /translation="MISWFARNHVASNLLMTGMTVAGLWSLLSGRIPLEVFPDMPSRI
                     VSVTVPYPGGTPEEVEESIVLPIEEAIEQVGGIKQINSTAGSSGGSVFIEVEEGRDTR
                     EVMEEVRGRLDTIPNFPELAERPVVQVDDGFHSVINVVIAADMAERDLRRLGEQVRDE
                     IAALPAITHCSLAGVRPYEIGIEIPEETLRKHGLTLSQVRDAIRASAVDLPAGVVRTD
                     AGDVSIRTRGRAYTGADYERVVIHTRPDGSKLTLGEVAVIQDGFNENPLIARLNGRRA
                     VIVTVQREAGQNAIHIADAVKSYVGEARGRLPAGVEIEFWNDRSKIVKGRIALLMQNA
                     QSSLILVLLFVGLFLRLDTVFWMALGMAASFMGALALMPFFDVTINLSSLFGFILVLG
                     IVVDDAIVVSEHVDTLRQRGLGMMEAAIRGTREVAVPIVFGVLTTVVAFLPMAWGASD
                     YLMMFKPIAIIFILVLLIALVETKIILPSHLAHRTPLVSRLADILDPVHRFSDAALHR
                     FVERFYRPALRACARHRYTAICGFVGGLVVLCAFFFSGRILWVFFPRVQSERIEARLA
                     MLEGTPFEVTEAHIQRICEIAEQMRKDYTGPDGRPVIRNILAAVGSTRVTYGSSSGGG
                     SSHLGEVTIETHGPEERSMKVNTVEMADDWRRRIGSIVGAEELTFRAEIMRGSDPVDI
                     QLTGTDPAELLALSEKIKAHLGTYSGVFDIGDSLDSGRAEIQLRLKPEAEQLGVTVGE
                     LARQVRQAFFGDEVQRIQRGRNEVRVMLRYPRDDRRNLATLETMRVRAAGGLEIPFAR
                     VAEASVGKSFTSIRRVDRRRALNITADVNKDTTDPAKVLAGAGAFIAELMAGHPHVQW
                     SFKGEAERQAEGAEAGKWALLFILMGIYTLIAIPFKSYAQPLMVMIVIPFGIVGSVLG
                     HLLHDMAVSSMSVCGMLAVTGVVVNDSIIMVDRVNRLREDGMAAFDAVVRGAASRFRA
                     ILLTSLTTYVGLMPLMYDFSGLISAMPPGVSHLLVLVFGDNSAAQSTSSQFLKPVSIA
                     IGYGCIFATLITLLLVPACYLAIEDLGKAWRWIKNEPPVKKRTPGPAMASAGE"
BASE COUNT          898 a         1770 c         1893 g          859 t
ORIGIN      
        1 gggcaggccg cgcgcctcgg ccacgatgcg ccgcgtcagg atgccggccg ctgtctccac
       61 ccggctctcg cgcgcgtctc cgagcgtggt ggagagcatg cccatcaccg ccagcagggc
      121 ggtggaggtg atgcccatgg ccatgatcgt ctccacgagg ctgaagcctg cggcatgagc
      181 tgcggaaggc gtggcgtggc gtgtcatggc aggagtacgg cctttccagt gttgcgttgc
      241 acggaaagcc accggcaata cgcgggatcg tggccgccgc cgctcatttt gaccgggatg
      301 ctgccgcgca cctccccctc ggccaccgcc agcagcaaag tgccgccgct ctccggctgc
      361 tccacctccc ccagcgtgcc gaagcggatg cagggcaggt ttcccatgcc cgcgggattg
      421 ctcgcggaaa agcgcacccg cttcgccgcc gcagggtggc tcatgaggtt ctgcccggcg
      481 ctctggagcg cgggcgcggt gagggtgaaa acgaagccgg tgggcaggtg ctcccagccg
      541 gtcagcggca gcaggatgct tcgctggccc tccggcaggt tgtcatcctc gatggagtag
      601 cagaccgcgt aggagcggaa ggcctgcgcc ggcgcccgcg cctcgccatt ggcaaaggcg
      661 atccatattt cctgctgccc ggccagcgcc ttcgcgcgtc cggagtccat cacggaaacg
      721 agctgctgca tcgcggtggt gccgcggttg ctcttcaagg aatccgtcac tcccgccgtc
      781 agcgccacca ccagggtgat gacgatgacc acgaacatca tctcgatcat cgtgtagccc
      841 ggcgcgtgcc ggacgtgcga cctgccaggc ggtggaaagc ttgatgtgtg acgcgggcgg
      901 cgcatgaggg cagggcgcgt ggccggggca aaatgcaccc ccgtcccggc acggcgaacg
      961 ctcatgtgtg tcattattgt cgaattcctg gcggcttgat tttcaacgaa ctgactttct
     1021 ttgagcagcc atcataccag gacgcccgta actgacaagc catccggtgg cagaattccg
     1081 ccggccttcc tcacccccgt catcttcccg tcatgctctc ccgccccgaa cccggaaaaa
     1141 aagcccccgc cttctccgcc cccgccgccg gtggcggcct caaaggcggc gccaaagtca
     1201 ccctcaaaga cttccaaggc cgccctcttg tgctctactt ttaccccaag gacgacaccc
     1261 ccggctgcac ccgccaggcc tgcgccctgc gcgacggctg ggcgcgcatc tcggagaaag
     1321 cgcaagtcct cggcgtcagc ggcgactcca tcgccagtca tgagaagttc atcaaaaaac
     1381 actcgctgcc ctttcccatc ctcagcgacg aggaccacgc cgtcgccagc gcctacggcg
     1441 tgtgggtgga gaaaagcatg tatggcaaaa aacacatggg catcgagcgc tccaccttcg
     1501 tcatcggccc cggcggcgtc atcagcgccg tcttccccaa agtgaagccc gacgagcatc
     1561 tggagcgggt tctcgcggct ctttgacttt ccctgcctat cctcgtcaat ccggtggatg
     1621 aatgagcggg ggcgctctca ttcgccggcg gacgccatcg cgggcccggg ggtccgcttt
     1681 ttgaccgggg gctcgttttt gatccagcgc caggctttgc ccaggtcctc gatggcgagg
     1741 tagcaggcgg ggacgagcag gagggtgatg agggttgcga agatgcagcc gtagccgata
     1801 gcgatggaca cgggtttgag gaactggctg ctggtggact gggcagcgct gttgtcgcca
     1861 aagacgagca cgagcaggtg gctgacgcct ggcggcatgg cggagatgag gccgctgaaa
     1921 tcgtacatca gcggcatgag gccgacatag gtggtcaggg aggtgagcag gatggcgcgg
     1981 aagcgcgagg cggcgccgcg aaccacggcg tcaaaggctg ccatgccgtc ctcccgcagg
     2041 cggttgacgc ggtccaccat gatgatgctg tcattgacca cgacgccggt gacggccagc
     2101 atgccgcaca cgctcatgct gctcacggcc atgtcgtgca gcaggtggcc gagcacggag
     2161 ccgacgatgc cgaaggggat gacgatcatg accatgagcg gctgcgcgta gcttttgaag
     2221 gggatggcga tcaatgtgta gatgcccatg aggatgaaaa gcagcgccca tttgcccgcc
     2281 tcggcgccct cggcctggcg ctcggcctcg cctttgaagc tccactgcac atgcgggtgc
     2341 ccggccatga gttcggcgat gaaggcgccg gcgccggcga gcaccttggc ggggtcggtg
     2401 gtgtccttgt tgacgtcggc ggtgatgttc agggcgcgcc ggcggtccac gcggcggatg
     2461 ctggtgaagc tcttgcccac gctggcctcg gccacgcgcg cgaaggggat ctccagcccg
     2521 ccggcggcgc gcacgcgcat ggtctccaga gtggcgaggt tccggcggtc gtccctgggg
     2581 tagcggagca tgacgcgcac ctcgttgcgc ccgcgctgga tgcgctgcac ctcgtcgcca
     2641 aaaaaagcct gccgcacctg ccgggccagc tcgcccacgg tgacgccgag ctgctcggcc
     2701 tcgggcttga ggcggagctg gatctcggcg cggccgctgt cgagcgagtc gccgatgtcg
     2761 aacacgcccg aataggtgcc caggtgcgcc ttgatctttt cggaaagggc cagcagctcg
     2821 gcggggtcgg tgccggtgag ctggatgtcc accgggtcgc tgccgcgcat gatctcggcg
     2881 cggaaggtca gctcctcggc gccgacgatg gagccgatgc gccgccgcca gtcgtcggcc
     2941 atctcgacgg tgttgacctt catgctgcgc tcctcggggc cgtgggtctc gatggtcacc
     3001 tcccccaggt gggagctgcc gccgccggag gaggagccgt aggtgacgcg ggtggagccg
     3061 acggcggcga ggatgttgcg gatcacgggc cggccgtcgg ggccggtgta gtctttgcgc
     3121 atctgctcgg cgatctcgca gatgcgctgg atgtgcgcct cggtcacctc gaagggcgtg
     3181 ccctccagca tggcgaggcg ggcctcgatg cgctcgctct gcacgcgggg gaagaagacc
     3241 cagaggatgc ggccgctgaa gaagaaggcg cacagcacca ccaggccgcc gacgaagccg
     3301 cagatggccg tgtagcggtg gcgcgcgcag gcgcgcaggg cggggcggta aaagcgctcc
     3361 acaaaccggt gcagcgcggc gtctgaaaaa cggtgcacgg ggtcgaggat gtcggccagg
     3421 cggctgacca gcggcgtgcg gtgggccagg tgggagggca ggatgatctt ggtctccacc
     3481 agggcgatga gcagcacgag gatgaagatg atggcgatgg gcttgaacat catcaggtag
     3541 tcactcgcgc cccaggccat gggcaggaag gcgacgacgg tggtcagcac gccgaagacg
     3601 atgggcacgg ccacctcccg cgtgccgcgg atggcggcct ccatcatgcc caggccgcgc
     3661 tggcgcagcg tgtccacatg ctcggagacg acgatggcgt catccaccac gatgcccagc
     3721 acgaggatga agccgaagag ggaggagagg ttgatggtga catcaaagaa cggcatgagc
     3781 gccagcgcgc ccatgaagct ggcggccatg cccagcgcca tccagaagac ggtgtccagc
     3841 cgcaggaaaa ggcccacgaa gagcaggacg aggatgaggc tgctctgcgc gttctgcatg
     3901 agcagggcga tgcggccttt gacgatcttc gagcggtcgt tccaaaactc gatctccacg
     3961 cccgcgggca gccgccctcg cgcctcgccc acgtagctct tcaccgcgtc ggcgatgtgg
     4021 atggcgttct gccccgcctc ccgctgcacg gtgacgatga cggcgcgcct gccgttgagc
     4081 cgcgcgatga gcgggttctc gttgaagccg tcctggatca ccgccacctc ccccagtgtc
     4141 agcttgctgc cgtccggacg ggtgtggatg accacgcgct cgtagtccgc gccggtgtag
     4201 gcgcgcccgc gcgtgcggat ggacacatcc cccgcgtccg tgcgcaccac gcccgcgggc
     4261 aggtccacgg cggaggcgcg gatggcgtcc cgcacctggc tgagggtcag cccgtgcttg
     4321 cgcagcgtct cctccgggat ctcgatgccg atctcatacg ggcgcacccc ggccagcgag
     4381 cagtgggtga tggccggcag cgcggcgatc tcgtcgcgca cctgctcgcc cagccggcgc
     4441 aggtcgcgct cggccatgtc cgcggcgatg accacattga tcacggagtg aaagccgtcg
     4501 tccacctgca ccaccggccg ctcggccagt tccggaaagt tcgggatggt gtccagcctg
     4561 ccgcgcacct cctccatcac ctcccgcgtg tcccgcccct cctcgacctc gatgaagacc
     4621 gacccgccgc tggaccccgc cgtggagttg atctgcttga tgccgcccac ctgctcgatg
     4681 gcctcctcaa tcggcagcac gatgctctcc tccacctcct ccggcgtgcc cccgggatag
     4741 ggcaccgtca ccgagacaat gcgcgagggc atgtccggga acacctccag cgggatgcgc
     4801 ccggagagca ggctccaaag cccggccacg gtcatgccgg tcatcagcag gttggaggcg
     4861 acgtgattgc gggcgaacca ggagatcatg ggcgggagtt gtgagggaaa acgcctggcg
     4921 gcggcaaaag tagccgcgcc tttgccgtgc cgcccgcgga tttccaaatg ccactgcccc
     4981 gcgcccccgg caaggcgcgc tttcagggcg cggccgggcg ccgcagccgc ccgcacgcca
     5041 ggttctgaag ggggcaaagc gggcctttcc cggtggctgc cggggctggc aacgggggtg
     5101 gggaacccgc cagcgggggc gcccaccctg gcaaggtggg cgggaagttt gaaaaagggg
     5161 gtgcccaccc tggcaaggtg ggcggggact ttgagaaggt gggcggcgac cctgacgagg
     5221 tgggagggga ctttgggaag gtgggcggcg accctgacgg ggtgggcgcg gctgccgggg
     5281 aacccgccag cggggcttca cggcttgatg cgggcgcagc cggaacaggg gcgcggattc
     5341 tcagccatcg tcgcgcaata gacgcttatc ccgaacgccg cttttgaaat gagatttgtt
     5401 aggggttgag gggtcagatc
//
LOCUS       DAOMYJ010000291         5400 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Prosthecobacter sp. isolate AMR_MDS_5629
            NODE_5224_length_5400_cov_22.111506, whole genome shotgun sequence.
ACCESSION   DAOMYJ010000291 DAOMYJ010000000
VERSION     DAOMYJ010000291.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867850
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prosthecobacter sp. (wastewater metagenome)
  ORGANISM  Prosthecobacter sp.
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Verrucomicrobiaceae; Prosthecobacter.
REFERENCE   1  (bases 1 to 5400)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5400)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.30
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:34:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,742
            CDSs (total)                      :: 4,684
            Genes (coding)                    :: 4,676
            CDSs (with protein)               :: 4,676
            Genes (RNA)                       :: 58
            tRNAs                             :: 55
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5400
                     /organism="Prosthecobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5224_length_5400_cov_22.111506"
                     /isolate="AMR_MDS_5629"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1965333"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(447..2189)
                     /locus_tag="PLP58_20200"
     CDS             complement(447..2189)
                     /locus_tag="PLP58_20200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ10915.1"
                     /translation="MAEHPEPAWRCPLMRGVPDNGDVRLPREMLEALLPHMQALGMTP
                     PDVADMVPKVRMDEPVRDLALRLGLLLKGSGLYRFGPNERLIYFDRGKPVRMTPVKFC
                     SWVERHVEVVKTAVGKSGAYDVRVSLGKDLAAKLLEADELTGELPLIEQIVATRIPVR
                     RRAGPLELCKVGYDEEAAIFCTNEVPFDEGWELGRALALLEDWCSEFSFAEAGAVGRD
                     LWQNRSFVVHVSAMLGVFGRRLLPAGTVRPMVIYLANDQGSGKSLLVSMVLAACFGVA
                     SSTDLPLSSRGPNQEKFTALLETVAQSMKEYLWIDDVPPAVFSNALNRFVTASAHTGR
                     KYGGNDEMFDAPAVTQVFMTGNNVELTRDIMQRSLVVELFMRTDSQKRTFKRRLTAMW
                     FCSRERRGEMLSALWAFFRAWSEAGMPMGQTLQERASDWSELVGGVLEAAGVTIDPFG
                     VPDLPMAGDRETEEWRALLTALADEAEAAPEFMESESSGRYEVDTDRIVSVARDNRLL
                     ADIVGTKDDKPLKGGELKRLGRRLAKWRGREDLVASSGRRFRFGKRKQNSNWVYPIEW
                     LDEPRDEESRGSAD"
     gene            complement(2150..3454)
                     /locus_tag="PLP58_20205"
     CDS             complement(2150..3454)
                     /locus_tag="PLP58_20205"
                     /inference="COORDINATES: protein motif:HMM:NF019878.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="primase-helicase zinc-binding domain-containing
                     protein"
                     /protein_id="HRJ10916.1"
                     /translation="MSEQPRTLPGVSRFFDASDVLDAARGRWPRVLADLGVRADLLTG
                     RHGPCPGCGGKDRFRFDDIEGNGSFVCSQGGAGLLAGNGVTLLMHVTGWEWKDAIEQL
                     GRRLLPDSMRRGFHRAAGGGGAGDARPPIVEDLPPEPVREDESRVPVYDEAKLRAYVA
                     SVPPVTRQMLKRVSPLKVQHATASDFLDVLYGDRERVLVFTEFFSQGNFLHQVGSGSC
                     RLSPDRGIKAVVSPLPTRGPEGVWYLCNPVDGRWQPSESRTRLAHLPAEMHGPPQRVV
                     EHGKWGRRTWRNVTTFRYAVLESDCAPEDLWMRALVKLPVPIAAIYSSGGKSLHALVR
                     VDAGSKPEWDLWVRGKGQGTHTRVASIMDLVCPLGADAAALTAVRLTRLPCCFREGVT
                     VRGEGYKRYPEPRMQELIYLNPMSFQRKPEWRSILSRHGGAR"
     gene            complement(3462..>5400)
                     /locus_tag="PLP58_20210"
     CDS             complement(3462..>5400)
                     /locus_tag="PLP58_20210"
                     /inference="COORDINATES: protein motif:HMM:TIGR00180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HRJ10917.1"
                     /translation="ELVAGERRLRAARLAGLETVPVIVYELDERAAQEIVLIENLQRE
                     DLTVTEEARGYEAALALRDDAGRPVYTQESLAAKLGKKVGYIRDRLKLLLCPELLITA
                     VEAGEVALSTAMLVGRIPGDKERVEAAKKVLSPTRQDVPLNYEQTREMIREEFMVSLQ
                     KPGFDLEDAELVPVRVDDDGSRIFGGACVGCPFAVKQEHALQMESPKNGAKIVSGSGT
                     MLCTLPSCFKKKQDAAWRIIARQAEAAGSRVLEGDAAAKVFNTWNGEVTDSAPYVALD
                     EAPGYTDLGMLSYENKKKWRSLLKGAELEVVVARHPKTGRRVELVERKPAQQIVRAKL
                     KGGGAAADVKDLAQAEAARKEQRAEDLRKAKLEKITLHECVGDVLASVGRKGVGVEEG
                     CLLFQMALDNGGADGMKFFKEWLGLKLPKGTARSGRDYEESILEAVRARAVSVNDWLG
                     FIVVAVIARGLAWQGCDNEDLELLMERYGVKKKEVARRAQALLDAGKKAPKGKRLDKA
                     DSDDRDDGVEVKPDLDLLERCRVWRAENPGRGAAALADALGLSYDEACRCVDALIDEA
                     APAEDPAAASADEGGGDEAADVAFKNQVRWIAEGSAKVGDYIGVKPKADEVEALAEWN
                     RERMRYNRAVKKYKQRPDLLR"
BASE COUNT          971 a         2178 c         1291 g          960 t
ORIGIN      
        1 aacgccgccc ctcagggcgc ccaccccccc tttcccaggc taaaagattc ctttctcaga
       61 tatgcgtctg ataaaatccc gagatgcata gtgacaagac cgccaagaaa agcgcaaaac
      121 agcccactct tgaaaaaacg tctttatcag accaatgcca ttttactcgg ctgtcttacc
      181 actcttgcca ctttccttga tttttcaagc ctcccatccg tggcaagaag tgttccaaga
      241 tgatttttaa gggcacgggt cttgccaccg ctgcgcccgc tcggcggtgg caagagagaa
      301 gcgtcagcat ccttttctat ggtgggcacg ggtagacagt ccgcgctacc gcgcgatcac
      361 ggaattccgc gcgctcttgc gcgagggccc cggcatgcgc gggcgtaact cgccggtgcc
      421 gcgcctggta cagcacgcac accggatcag tccgcgctac cgcgcgactc ttcatcgcgt
      481 ggctcatcaa gccactcgat gggatagacc caattcgagt tctgcttccg tttgccgaag
      541 cggaatctgc ggccgctact tgccaccaaa tcttcacggc cgcgccactt cgccaggcgt
      601 cgccccaacc gcttcagctc gccacccttc aacggcttgt catccttcgt ccccacaatg
      661 tcagctagga ggcgattgtc tcttgccact gacacgatgc gatccgtgtc cacctcataa
      721 cggccactgc tttcagactc catgaattcg ggcgcagcct cagcctcgtc cgccagcgcg
      781 gtcagcagcg cgcgccattc ctccgtttcc cggtcgcccg ccatcggcag atccggcacc
      841 ccgaaggggt caatcgttac cccagccgcc tccagcacgc cccccaccag ctccgaccag
      901 tcggatgccc gctcctgcag cgtctgtccc atcggcatgc ccgcctcaga ccaggcccga
      961 aagaaagccc acaacgccga gagcatctcg ccacgccttt cgcgcgagca aaaccacatc
     1021 gcggtgagcc ggcgcttgaa tgtccgcttc tgcgagtccg tgcgcatgaa aagctccacc
     1081 acaagcgagc gctgcataat gtcgcgcgta agctccacgt tatttccggt catgaaaact
     1141 tgagtcacag ccggcgcgtc gaacatttca tcgttcccgc catacttgcg gcctgtgtgc
     1201 gccgacgccg tcacaaagcg gttaagtgcg ttcgaaaaaa ccgccggcgg cacgtcgtca
     1261 atccacaaat actccttcat gctctgcgcc accgtctcca gcagcgccgt gaatttctcc
     1321 tggttcggcc cgcgcgaact cagcggcaga tccgtgctcg acgccacgcc gaagcacgcc
     1381 gccagcacca tgctcaccag cagactcttg ccgctgccct ggtcgttcgc caggtagatc
     1441 accatcgggc gcaccgtccc cgccggcagc agccgcctgc cgaacacccc cagcatggcc
     1501 gaaacgtgga ccacaaagct cctgttctgc cacagatccc gccccaccgc ccccgcctcc
     1561 gcgaaactaa actccgagca ccaatcctcc agcagagcga gcgccctccc cagctcccac
     1621 ccctcatcaa acggcacctc gttggtgcaa aaaatcgccg cctcctcatc ataccccacc
     1681 ttgcacagct ccagcgggcc cgccctgcgg cgcacaggga tgcgcgtcgc cacgatctgt
     1741 tcgatgagcg gcagctcgcc agtaagttca tccgcctcca gcagcttcgc cgccagatcc
     1801 ttccccagac tcaccctcac atcatacgcg ccgctcttgc ccacagccgt cttcaccacc
     1861 tccacatgcc gctccaccca cgagcaaaat ttcaccggcg tcatccgcac cggcttgccc
     1921 cggtcaaaat aaatcaaccg ctcattcggc ccgaacctgt agagaccgct ccccttcagc
     1981 agcaggccca ggcgcagcgc cagatcgcgc acaggctcgt ccatgcgcac cttcggcacc
     2041 atgtccgcca catcgggtgg cgtcatcccc agagcctgca tgtgcggcag cagagcctcc
     2101 agcatctcgc gcggcaggcg cacatccccg ttgtccggca cgcccctcat cagcgggcac
     2161 ctccatgccg gctcaggatg ctccgccact caggctttct ctggaaactc atcggattca
     2221 gataaatcaa ttcctgcatc ctcggctcag gataccgctt gtaaccttcc ccgcgcaccg
     2281 tcacaccctc gcgaaagcag cacggcagcc gggtcagccg cactgccgtc aatgccgccg
     2341 cgtccgcacc cagagggcac accaggtcca tgatggacgc cacccgcgtg tgcgtcccct
     2401 gccccttgcc gcgcacccac agatcccact ccggcttgct ccccgcgtcc acccgcacca
     2461 gcgcgtgcag gctcttgccc ccgctcgaat agatcgccgc aatcggcacc ggcagcttga
     2521 ccagcgcccg catccacaaa tcttccggcg cgcagtcgct ctccagcacg gcataacgaa
     2581 atgttgtcac attccgccac gtccgccgcc cccacttgcc gtgctccacc acacgctgcg
     2641 gcggcccatg catctccgcc ggcagatgtg ccaggcgcgt gcggctctct gagggctgcc
     2701 accgcccatc cacagggttg cacagatacc acaccccctc tggcccccgc gtcggcagcg
     2761 gcgacaccac cgccttgatt ccgcgatccg gcgacagccg gcagctcccg ctccccacct
     2821 ggtggagaaa attgccctgc gagaaaaatt ccgtgaaaac cagcacccgc tctctgtcac
     2881 cgtagagcac atcgaggaaa tccgacgccg tcgcatgctg caccttcagc ggcgacaccc
     2941 gcttcagcat ctgcctcgtc acaggcggca cgctcgccac ataggcacgc agcttcgcct
     3001 cgtcatacac cggcacgcgc gactcatctt cccgcacagg ctcgggaggc agatcctcca
     3061 caatcggcgg ccgcgcatcg ccagcccccc cgccgcccgc cgcgcgatga aacccccgcc
     3121 tcatcgagtc cggcagcagc cgccgcccga gctgctcgat ggcgtccttc cattcccatc
     3181 ccgtcacgtg catgagcaac gtcaccccgt tgcccgccag caggcccgca ccgccctggc
     3241 tgcacacaaa cgagccattc ccctcgatgt catcgaagcg gaagcggtcc ttgcccccgc
     3301 agccagggca cgggccatgc cggccagtca gcagatccgc gcgcaccccc aagtccgcca
     3361 gcacacgcgg ccatcggccg cgcgccgcgt ccagcacatc gcttgcgtcg aaaaatctgc
     3421 tcacaccagg cagagttcgg ggctgctcgc tcatgttgca tttacctcag taaatcaggc
     3481 cgctgtttgt atttcttcac agcgcggttg tatctcatcc gctcgcggtt ccactcggcc
     3541 agagcctcca cctcgtccgc cttgggcttg acgccgatgt agtcccccac cttggccgac
     3601 ccctccgcga tccagcgcac ctggtttttg aaggcgacat ccgcagcctc atcgcccccg
     3661 ccctcatcag cagaggccgc agccggatct tccgccggcg ccgcctcatc aatgagcgcg
     3721 tccacgcacc ggcacgcctc atcatagctc agaccaagcg cgtccgccag cgccgccgca
     3781 ccgcgtccgg ggttctccgc gcgccacacc ctgcaccgtt ccagcagatc cagatctggc
     3841 ttcacctcca cgccgtcatc ccggtcatca gagtcagcct tgtccagccg cttccccttc
     3901 ggcgccttct tccccgcgtc cagcagcgct tgagcgcggc gcgccacctc cttctttttc
     3961 accccgtatc gctccatgag cagctccaga tcctcgttgt cacagccttg ccaggccagc
     4021 ccccgcgcga tgaccgccac aacgatgaag cccagccagt cattcacact caccgcccgc
     4081 gcgcgcaccg cttccaggat gctttcctca taatcacgcc cgctcctcgc agtgcccttc
     4141 ggaagtttca ggcccagcca ttccttgaaa aacttcatgc catccgcgcc accgttgtcc
     4201 aaggccattt gaaagagcag gcacccctcc tccacgccca cacccttgcg ccccacagac
     4261 gccagcacat cccccacaca ctcatgcagc gtgatcttct ccagcttcgc cttccgcaga
     4321 tcttccgccc gctgctcctt ccgcgccgcc tccgcctgcg ccaggtcctt cacatccgcc
     4381 gcagcacccc cacccttgag cttcgcccgc acgatctgct gcgcaggctt ccgctccacc
     4441 agctccaccc tccggcccgt cttcgggtgg cgcgccacca ccacctccag ctccgcgccc
     4501 ttcagcagcg accgccactt cttcttgttc tcataggaga gcattcccag gtccgtataa
     4561 cccggcgcct catccagcgc cacgtaaggt gccgagtcag taacctcccc gttccacgtg
     4621 ttgaaaacct tcgccgcagc gtccccctcc agcacccgac tccctgccgc ctccgcctgc
     4681 cgcgcaatga tccgccaggc cgcgtcctgc ttcttcttga agcaggaggg cagggtgcag
     4741 agcattgtgc cgcttccaga tactattttt gcaccgtttt ttggggattc catttgcaag
     4801 gcatgctcct gtttcacagc aaacgggcac cccacacacg ccccgccaaa aatccggctc
     4861 ccgtcatcat ccaccctcac aggcaccaac tccgcatcct ccagatcgaa gcccggcttc
     4921 tggagcgaga ccatgaattc ctcccggatc atctcccgcg tctgctcata gttcagcggc
     4981 acatcctggc gggtcggcga gagcaccttc ttcgccgcct ccacccgctc cttgtcgccc
     5041 gggatgcgcc ccaccagcat cgcggtcgag agtgccacct cacccgcctc caccgccgtg
     5101 atcagcagct ccgggcacag cagcagcttc aggcggtcgc ggatgtagcc cacctttttc
     5161 cccagcttcg ccgccaggct ctcctgcgtg taaaccggcc ggcccgcgtc atcccgcagc
     5221 gccagcgccg cttcataacc gcgcgcctct tcggtcacgg tgagatcctc gcgctgcagg
     5281 ttttcgatca acacgatctc ttgcgccgcg cgctcatcca gctcatacac gattaccggc
     5341 actgtctcca gtcccgccag ccgcgccgcg cgcaggcgcc tctcgcccgc caccagctcc
//
LOCUS       DAOMYJ010000292         5375 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Prosthecobacter sp. isolate AMR_MDS_5629
            NODE_5260_length_5375_cov_20.375000, whole genome shotgun sequence.
ACCESSION   DAOMYJ010000292 DAOMYJ010000000
VERSION     DAOMYJ010000292.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867850
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prosthecobacter sp. (wastewater metagenome)
  ORGANISM  Prosthecobacter sp.
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Verrucomicrobiaceae; Prosthecobacter.
REFERENCE   1  (bases 1 to 5375)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5375)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.30
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:34:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,742
            CDSs (total)                      :: 4,684
            Genes (coding)                    :: 4,676
            CDSs (with protein)               :: 4,676
            Genes (RNA)                       :: 58
            tRNAs                             :: 55
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5375
                     /organism="Prosthecobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5260_length_5375_cov_20.375000"
                     /isolate="AMR_MDS_5629"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1965333"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2989)
                     /locus_tag="PLP58_20215"
     CDS             complement(<1..2989)
                     /locus_tag="PLP58_20215"
                     /inference="COORDINATES: protein motif:HMM:NF012200.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choice-of-anchor D domain-containing protein"
                     /protein_id="HRJ10918.1"
                     /translation="MTLFEYNYHIQTIHSNNSQAATPHCSLMLPFPSSIRQFAAQSGC
                     LTLIWTLAGILAWPLAAQDFQPAYDLAKPPGASLDAPSILAHGKLPGDGFGRVIRTGD
                     VNGDGIEDLIVGSATASAGATRVNAGTVCIWFGSGQIGGVFDAASSVGRAPDVTLLGA
                     RAGDSLTSGGALIVADVSGDGIADILLGAPGADGPSASRDGAGESYIVFGRDIFPNVL
                     DLAVQGDGGADVTIHGASEGDSLTSGGALTAGDVNGDGITDLLLGALAADGPDESRPS
                     AGEAYVIFGREEFTFTLDLGIQGPGGADVTLYGADEGDFLAYDGAVKAADVNADGIPD
                     ILLGAVGADGPDETREAAGECYIVFGRPGLPPVLDLKISGPGNADVTLYGVSPDDQMT
                     SGGSIVVADVNGDGIDDLLLGTPGGDGPNDTRSGAGESYIVLGRPFFPPVMDLAFQGH
                     GGADVTLYGASAEDSLTSGGALAAADVDKDGIADILLGAGLADGPEESRLSAGEACIV
                     LGRPVFPVTLDLAIQGPGGADVTLYGASPGDNLAIGGALLAADVNQDGRADLILGALL
                     ADGPEEGLSNAGEACVILGRDAFPPVLDLAIHGPGGADITLYGATANDQLTRERSLAA
                     GDINGDGIPDILLGARDADGPGEARGGAGEVTVILGSGIPFTGPQITLHQPAGTYLAD
                     GGGRYFGAVLLNNIGTRLFTVVNTGTEPLTGIAITLGGVNEDDYSVDRAALPAELAPG
                     QSGAFLVTFAPGTLGLRAAAMQIHSSDTSRSPYDVRLTGIGAAPELSVEQPAGSPRHT
                     GSTRAYGGVATGYSKTLVFVLKNIGTADLEGVIPSLTGADAARFSFPAPASSTIAPGQ
                     SLSLPVTFTPGVLGDFSAALHVISSVSPDAPYVVHLTGSGVPPAPELDLEQPAHTVLE
                     SGGARAYGSVSIGASKNLVFTLRNTGTAPLTGIAFSTAGENPADFLPLSPTLPDVPPG
                     GSAAFTLAFAPQARGARAAELRIA"
     gene            3128..4621
                     /locus_tag="PLP58_20220"
     CDS             3128..4621
                     /locus_tag="PLP58_20220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009961879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase"
                     /protein_id="HRJ10919.1"
                     /translation="MRLPTAFFLTVVCALGALLPGAEAARPNVLFVLTDDQRWDAIGL
                     GGSKHLKTPNMDRIGREGVHFKNTFCTTSLCSPSRASILSGMYAQKHGVTNNFTEYPE
                     GFKSFPMVLQGAGYDTAYIGKWHMGEDNDEPRPGFNWFVTHKGQGKYFDTEFNINGQG
                     AKVVPGYYTHVVTGMAEEWLGRERGDKPWCLMIGHKAPHSFYFPEPKYEKSFENARIP
                     YPETAFMLEDKPKWITERLYTWHGIYGPLFEWRKKFPDDSPEAVKDFEAMTRAYWGTL
                     LSVDDSMGRLYELLKKRGELDNTIIVLMGDNGILNGEHGMVDKRTMHEASIRIPLVVR
                     YPGLVPADKPVVVEQQVLTLDMAPSILELCGAPALEGIHGRSWVRLAREGDPGWRGSW
                     LYHYNYEKQFPYTPNVRGVRTESWKYVHYPHGDGTPDRHMAELYNIEFDPEERHNLIA
                     NPKYASVVAEMKNELARVMAGAGLTAENDKMPLDEGIKSELPDAKIR"
     gene            4748..>5375
                     /gene="scpB"
                     /locus_tag="PLP58_20225"
     CDS             4748..>5375
                     /gene="scpB"
                     /locus_tag="PLP58_20225"
                     /inference="COORDINATES: protein motif:HMM:NF016005.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HRJ10920.1"
                     /translation="MELTAIVESLLFATQEPLLLEKMAAAVKETAKDIKEAAGEQPPE
                     WLEPLLAVDEAGIREAIDQLTAHYEKDGRAFTIVERSHGWRLCARIEFAEWCRALYPG
                     KKVQRLSQPALETLAIIAYRQPITKAGIEAVRGVSVDAMVQQLVDRGLAKIEGRADLP
                     GKPLLYGTTDAFLDHFGVRTLDELPNAAELRRVKLPSPESGQASPAAEE"
BASE COUNT          967 a         1648 c         1917 g          843 t
ORIGIN      
        1 tggcgatgcg cagctccgcc gcgcgcgcgc ccctggcctg gggggcgaag gcgagggtga
       61 atgcggcgct gccgccgggg ggcacgtcgg gcagggtggg ggagaggggc aggaagtcgg
      121 cggggttttc ccccgcggtg ctgaaggcga tgccggtgag gggcgcggtg ccggtgttgc
      181 gcagggtgaa gacgaggttt ttcgacgcgc cgatggagac gctgccatag gcgcgggcgc
      241 cgccgctttc caggacggtg tgcgcgggct gctcgaggtc cagctcgggc gcgggcggca
      301 cgccggagcc ggtgaggtgg acgacgtaag gcgcgtccgg cgagacgctg ctgatgacgt
      361 gcagggcggc gctgaagtct ccaagcacgc cgggggtgaa ggtgacgggc aggctcaggc
      421 tctggccggg ggcgatggtg ctggaggcgg gcgcggggaa gctgaagcgg gcggcgtccg
      481 ccccggtgag agaggggatc acgccttcga gatcggcggt gccgatgttt ttgaggacaa
      541 agacgagggt cttgctgtaa ccggtggcca ccccgccata ggcgcgcgtg gagccggtgt
      601 ggcgcgggct gccggcgggc tgctcgacgg agagctcggg cgcggcgccg atgccggtga
      661 ggcgcacatc ataggggctg cggctggtgt cgctgctgtg gatctgcatg gcggcggcgc
      721 gcaggccgag ggtgccgggg gcgaaggtga cgaggaaggc accgctttgg ccgggggcaa
      781 gctcggcggg cagggcggcg cggtccacgg agtagtcgtc ctcattgacg ccgccgaggg
      841 tgatggcgat gccggtgagg ggctcggtgc cggtgttgac gacggtgaaa aggcgggtgc
      901 cgatgttgtt gagcagcacc gcgccaaagt agcgcccgcc gccgtcggcc aggtaggtgc
      961 cggcgggctg gtgcagggtg atctgcgggc cggtgaaggg gatgccgctg ccgaggatga
     1021 cggtgacttc gccggctccg ccgcgcgcct cgccggggcc gtcggcatcg cgcgcgccga
     1081 ggaggatgtc ggggatgccg tcgccattga tgtcgccggc ggccagggac cgctcgcggg
     1141 tgagctggtc attggcggtg gcgccataaa gggtgatgtc cgcgccgccg ggtccatgaa
     1201 tggcgaggtc gagcacgggc gggaaggcat cgcggccgag gatgacgcag gcctcgccgg
     1261 cgttggagag gccttcctcc ggtccgtcgg ccagcagcgc gccgaggatg agatcggcgc
     1321 gtccgtcctg gttcacatcc gccgcgagca gcgcgccgcc gatggcgagg ttgtcgccgg
     1381 gggacgcgcc atagagggtg acatccgcgc cgccggggcc ctggatggcg aggtcaagcg
     1441 tgacgggaaa aacgggccgg cccaggacga tgcaagcctc gcccgcgctg aggcggcttt
     1501 cctccggccc gtcggccagt cccgcgccga ggaggatgtc ggcgatgccg tccttgtcca
     1561 catccgcagc ggccagcgcc ccgccggagg tgaggctgtc ctcggcggag gcaccgtaga
     1621 gggtgacatc cgcgccgccg tggccctgga aggccaggtc catgaccggc gggaagaagg
     1681 ggcggccaag aacaatgtag ctttcgcccg cgccgctgcg ggtgtcattc ggaccgtcgc
     1741 cgccgggtgt gccgagcagg agatcgtcaa tgccgtcgcc attgacatcg gccacgacaa
     1801 tggagccgcc ggaggtcatc tgatcgtcag ggctgacgcc gtaaagggtg acatccgcgt
     1861 tgccggggcc gctgattttc agatcaagga cgggaggcag tcccggcctg ccaaagacga
     1921 tgtagcattc gcctgcggct tcgcgtgttt catcgggacc gtccgcgccg accgcgccga
     1981 ggaggatgtc cggtatgccg tccgcattga catccgccgc tttgaccgcg ccgtcatagg
     2041 ccaggaaatc gccctcgtcc gcgccgtaga gggtgacatc agcaccgccg gggccttgaa
     2101 tgccgaggtc cagcgtgaag gtaaactcct ccctgccgaa gatgacataa gcctcgcccg
     2161 cgctgggtcg cgactcgtcc gggccatcgg cggccagtgc gccaaggagc aggtcggtga
     2221 tgccgtcgcc attgacatcc cccgcagtca aagcgccgcc ggaggtgagg ctgtccccct
     2281 cgctggcacc gtggatcgtg acatccgcgc ctccatctcc ctggacggcc aggtcgagca
     2341 cattgggaaa aatgtcgcgg ccaaagacaa tatagctttc ccccgcgcca tcgcggctgg
     2401 cggaggggcc gtccgcgccg ggggcgccga ggaggatgtc ggcgatgccg tcgccactca
     2461 catccgccac gatgagagcg ccgccggaag tgagactatc gcctgcgcgc gcgcccagca
     2521 gggtcacatc cggcgctctg ccaacggagg aggcggcatc aaacacgccg ccgatttgcc
     2581 cgctgccgaa ccagatgcag actgtgcccg cgttgacccg ggtagccccg gcgctcgcgg
     2641 tggctgagcc gacaatcaag tcttcaatac cgtcaccatt gacatccccg gtgcggatga
     2701 cgcggccaaa gccatcgccg ggcagcttgc catgcgccag aatggagggg gcgtccaggg
     2761 aggcgccggg gggttttgcc agatcgtagg caggctggaa atcttgagcg gccaggggcc
     2821 aagccaaaat cccggcgagt gtccagatca gagtcagaca gccggactgg gctgcaaact
     2881 ggcggatgct ggacgggaag gggagcatga gagagcagtg aggagtcgcg gcctgggaat
     2941 tgttcgagtg gatcgtctga atatggtaat tatattcaaa taaggtcatt cacaagcatt
     3001 gattctcaat tttgagataa accgggccat tttgagccaa gcaaaagggt atgcctcaag
     3061 gcgcccggcc tgatccgggg agaatgcgtt tgcaagaatc cgcctcccgt gcgttttgcg
     3121 cactgccatg agactgccaa ccgccttttt tctcactgtc gtctgcgctc tcggggcgct
     3181 cctgcctggc gctgaagccg cgcgcccgaa cgtgctcttt gtcctgaccg acgaccaacg
     3241 ctgggatgcc atcggccttg gtggcagcaa gcatctcaag acgccgaaca tggaccgcat
     3301 cggcagggaa ggggtgcatt tcaaaaacac cttctgcacc acctcactct gctcgcccag
     3361 tcgcgccagc attctcagcg gcatgtatgc gcaaaagcac ggggtgacga acaacttcac
     3421 cgagtatccc gagggcttca aaagcttccc catggtgttg caaggcgcgg gctatgacac
     3481 cgcctacatc ggcaagtggc acatgggcga ggacaacgac gagccgcgcc ccggcttcaa
     3541 ctggttcgtg acgcacaagg ggcagggcaa gtatttcgac accgagttca acatcaacgg
     3601 ccagggcgcg aaggtggtgc cgggttatta cacgcatgtc gtcaccggga tggccgagga
     3661 atggctgggc cgcgagcgcg gggacaagcc ctggtgcctg atgatcgggc acaaggcgcc
     3721 gcacagcttt tacttcccgg aaccgaagta tgaaaagtcc tttgaaaacg cgcgcatccc
     3781 gtatcccgag acggccttca tgctggagga caagccgaaa tggatcaccg agcggcttta
     3841 cacctggcac ggcatttacg ggccgctctt cgaatggcgg aaaaaattcc ccgacgacag
     3901 ccccgaggcg gtgaaggact tcgaggccat gacgcgcgcc tactggggca cgctgctctc
     3961 cgtggacgac agcatgggcc gcctttatga gctgctgaaa aagcgcggcg agctggacaa
     4021 caccatcatc gtcctcatgg gcgacaatgg catcctcaac ggcgagcacg gcatggtgga
     4081 caaacgcacc atgcacgagg ccagcatccg catcccgcta gtcgtgcgct accccggact
     4141 cgtcccggcg gacaagccgg tggtcgtaga gcagcaggtg ctcaccctcg acatggcgcc
     4201 cagcattctc gaactctgcg gcgcgcccgc gctggagggc atccacggca gatcgtgggt
     4261 caggctcgcg cgtgaaggcg accccggctg gcgcgggagc tggctctatc attacaacta
     4321 cgagaagcag ttcccctaca cgccgaacgt gcgcggcgtg cgcaccgagt cgtggaagta
     4381 tgtgcattat ccgcatggcg acggcacgcc ggaccgccac atggccgagc tttacaacat
     4441 cgagttcgac ccggaagagc ggcacaacct gatcgccaat cccaaatacg cctccgtggt
     4501 cgcggagatg aaaaacgagc tggctcgtgt catggccggt gccggcttga cagccgaaaa
     4561 cgacaagatg ccgctggatg agggcatcaa gtccgaactg ccggacgcga agatccggtg
     4621 agcgtggcgg gagcggcttt ttttccatgc gaggcggtgc gatgtgcttt ccgagcaggc
     4681 gcgctcaacc tcgcgcccaa gcgtggcttg actctccccc cgtgcttgct tggatgggcg
     4741 gcccatcatg gaactgaccg ccatcgtcga aagccttctt tttgccaccc aggagccgct
     4801 gcttctggaa aaaatggcgg cggcggtcaa agagacggcc aaggacatca aggaggccgc
     4861 cggcgagcag ccgccggagt ggctggagcc gctcctggcg gtggacgagg ccggcatccg
     4921 cgaggccata gaccagctca ccgcgcacta cgagaaggac gggcgcgctt tcacgattgt
     4981 ggagcgcagc cacggatggc ggttgtgcgc ccggatcgag tttgccgagt ggtgccgcgc
     5041 gctttatccg ggcaaaaaag tccagcgcct cagccagccg gcgctggaga cgctggcgat
     5101 catcgcctac cgtcagccga tcaccaaagc gggcatcgag gcggtgcgcg gcgtgagtgt
     5161 ggacgccatg gtgcagcagc tcgtggaccg gggcctggcg aagatcgaag gccgcgccga
     5221 tctgcccggc aaacccttgc tctatggcac gacggatgcc tttctcgacc atttcggcgt
     5281 gcgcacactg gacgagctgc caaatgccgc cgaactgcgc cgcgtgaagc tgccctcgcc
     5341 ggaatccggg caggcctccc cggcagcgga ggaga
//
LOCUS       DAOMYJ010000293         5360 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Prosthecobacter sp. isolate AMR_MDS_5629
            NODE_5278_length_5360_cov_20.204147, whole genome shotgun sequence.
ACCESSION   DAOMYJ010000293 DAOMYJ010000000
VERSION     DAOMYJ010000293.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867850
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prosthecobacter sp. (wastewater metagenome)
  ORGANISM  Prosthecobacter sp.
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Verrucomicrobiaceae; Prosthecobacter.
REFERENCE   1  (bases 1 to 5360)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5360)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.30
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:34:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,742
            CDSs (total)                      :: 4,684
            Genes (coding)                    :: 4,676
            CDSs (with protein)               :: 4,676
            Genes (RNA)                       :: 58
            tRNAs                             :: 55
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5360
                     /organism="Prosthecobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5278_length_5360_cov_20.204147"
                     /isolate="AMR_MDS_5629"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1965333"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..557)
                     /locus_tag="PLP58_20230"
     CDS             complement(<1..557)
                     /locus_tag="PLP58_20230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ10921.1"
                     /translation="MPLPRILLVFGAFAREGALKAVAGWSRAVVLEVDAFLPSPQREQ
                     CAAIVTVPPLLPEAEAMRLQNRAEDLLDEFLDERAPQSGLETSRPFRASCLSIEAWRI
                     VIPHIVCLEYASRALEHGPFAEIIVAPGAGVSLQAFKQLAGALGAPLRVLPHDRQKPP
                     LLWMLKRRWQRARLKAQAAPPPPPRL"
     gene            complement(557..1588)
                     /locus_tag="PLP58_20235"
     CDS             complement(557..1588)
                     /locus_tag="PLP58_20235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ10922.1"
                     /translation="MKPPLFIHIGTHKTGSTAIQRALPVGGAAALKEGLVRLDHHGDI
                     SRANWTDADAEALASGFRRQMLERPVLYLLTCEGFCGDPYQGYAGASLIASRLRAATL
                     DFDVSIVVFLRRQDDFIESIYTQLIHQGGSLSFDAFLRSLPPGGLDWHRLLETYAAEF
                     GRERLIVRRYHHDFYPKPESLLEDFCSILGVSPASLEKKKASATPNQGYSREAQEIAL
                     LCNAHLDADDRKALRSLLQKISPKPVFQNYSYLDLPARQRLLETFAAGNAAVLRDYFC
                     GGTGGALFPPPEAVPDTDSPSDVVPLTVILVKMLLDLHQTQRGSRLVRLAASLDKALR
                     RMRGHSTSR"
     gene            1717..3123
                     /locus_tag="PLP58_20240"
     CDS             1717..3123
                     /locus_tag="PLP58_20240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009959695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ10923.1"
                     /translation="MRTLLPACIPALFTVMLQAAAPVPLYIDGYAGKISVAQGEKVPL
                     HVSTTAEKYDIEVARLGRERVVVWKQAGVPGAEHPVPEDASAQGCRWPVSVEVPVGAD
                     WRSGYYEISLRAADRGGKWTARGARTASDGAFFIGGQATPGSASKILLQLATNTYNAY
                     NNWGGFSVYAYNSRGNNQGSRVSFERPPASQFSRWELPFVQWAESAGHTLEFAANADL
                     EFHPGMLQSYKLVLSVGHDEYWSTPMRDHLEDFIGKGGNVCFFSGNTCCWQVRSEDDG
                     RAFTCWKQNYFQDPVFKTSDHRTLSTLWSHHLLKRPETQLTGVGFLWGGYHRSHGQFM
                     DGKAAFTVHRPAHWIFEGTGLKRGDEFGGKDSIVGYECDGCELEWRDGLPFPTHRDGS
                     PPTFEVLGTCEARWHPDDAEWYEKWEQGRTGAACMGVHERGGIVFTAGTTDWAHGLSG
                     GDATVTRITRNLLDRLSK"
     gene            3205..4179
                     /locus_tag="PLP58_20245"
     CDS             3205..4179
                     /locus_tag="PLP58_20245"
                     /inference="COORDINATES: protein motif:HMM:NF012970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HRJ10924.1"
                     /translation="MQPPLWMKSPALRPLLRLCAVLALLVAPALAQAQSAVLSADTFN
                     RKVHVAAGGNTRRAVGGLAKIATAMVTLDWAEASRQGVSVLATVPEYAPRVAGHNTLG
                     LQPGDKATLRDLICATLMTSDDIAAITLGEFVGRDHLYRLGRSGVPLDEFARQMNQLA
                     AREGATNTRFTNPHGYENTRAASYSTAADIARLALYAVSRPALKFYTNQRSRGISVYR
                     SGGQVSIPITNTNPLLGVSGIDGLKYTSTPRSGGCIVVTAERPSSVIRQADGSSLIYR
                     HRMVVVVLGSRDPGAEAHAVLQQSWGAYDRWLAAGRPITDTRQLLNHY"
     gene            4261..5352
                     /locus_tag="PLP58_20250"
     CDS             4261..5352
                     /locus_tag="PLP58_20250"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009961506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003872 - 6-phosphofructokinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006002 - fructose 6-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent 6-phosphofructokinase"
                     /protein_id="HRJ10925.1"
                     /translation="MRIGILNSGGDCPGLNAVIHGVVGAAHTLGWEVVGFRDGFEGLL
                     PPGDYMMLDPSKTVGIMKLGGTILGTTNKGHFVAKVGEGNVAEVPAEIVEKARKILDQ
                     LEIRALIVVGGDGSLTTGLQLYKMGIPVIGVPKTIDNDIQATAMTFGFDSACHSVVDA
                     LDRLHTTAESHKRVIVLEVMGRHAGWIALYGGISGGADVILLPEIPFHMEHVADAIRK
                     RDARGLHSTLVVVAEGARMESGELLKKEDVGGKGEDRLGGIGEHVAKRIQELTGKETR
                     ACTLGHLQRGGAPTALDRILGVRFGAKAVHLIEEGKFGRMVSYQHYNVGDVSIEEAVN
                     QLRLVDPGSEIIRAGRSIGVCFGDRAVGA"
BASE COUNT          947 a         1819 c         1767 g          827 t
ORIGIN      
        1 agccggggcg ggggaggcgg cgcggcctgc gccttgagcc gggcgcgctg ccatcggcgt
       61 ttgagcatcc agagcagggg cggcttttgc cggtcatgcg gcagcacacg caggggcgct
      121 ccgagcgcgc cggcgagctg cttgaaggct tgcagactga cgcccgctcc gggcgcgacg
      181 atgatttcgg caaatgggcc gtgctccagg gcgcggctgg catattccag gcagacgatg
      241 tgcggaatga caattcgcca ggcttcaatg ctcaggcagg aggcgcggaa cggacggctg
      301 gtttccaggc cggactgcgg cgcgcgctca tcaagaaact cgtcgagcaa atcctcggcg
      361 cggttttgca gccgcatcgc ctcggcttcc ggcagcaagg gcggcacggt gacgatggcg
      421 gcgcattgtt ccctctgcgg agacggcaaa aaggcgtcca cttccaggac gacggcgcgg
      481 ctccagccgg ccactgcttt caaggcgcct tcccgggcga aggcgccgaa cacgaggagg
      541 atccgtggca gcggcatcag cgggaagtgg agtggccgcg catccggcgc agggccttgt
      601 ccagactggc ggccaggcgg acgagccgcg agccgcgctg ggtttggtgg aggtcgagca
      661 gcatcttgac caggatgacg gtcagcggca caacgtcact aggagagtcg gtgtccggaa
      721 ctgcctcggg cggcgggaaa agcgcgccgc cggtgccgcc gcagaagtag tcacggagga
      781 cggcggcatt tcccgcggcg aaggtctcca gcaggcgctg gcgcgcgggc aggtcgagat
      841 acgagtagtt ttggaagaca ggcttggggc tgattttttg cagcagggag cggagcgctt
      901 tgcggtcgtc agcgtcgaga tgggcgttgc acagcagggc gatctcctgg gcttcgcgcg
      961 agtagccctg gttcggggtg gcggaggctt ttttcttttc caatgaggcg gggctgacgc
     1021 cgaggatgga gcaaaagtcc tccagcaggc tctccggctt cggataaaaa tcgtggtgat
     1081 aacgccggac gatgaggcgc tccctgccaa actcggcggc ataggtctcc aggagccggt
     1141 gccagtccag tccaccgggg ggcagcgatc gcagaaaagc gtcaaaactc agcgagccgc
     1201 cctggtgaat gagctgggtg taaatggact cgatgaagtc atcctgccgg cgcaggaaga
     1261 cgacgatgct cacgtcgaaa tccagggtgg ccgcgcgcag ccgggaggcg atgagcgacg
     1321 cgccggcgta gccctggtag ggatcgccgc agaagccctc gcaggtgagg aggtaaagta
     1381 cgggacgctc cagcatctgc cggcggaaac cggacgcaag cgcctcggcg tcagcgtccg
     1441 tccagttggc gcgggaaatg tcaccatgat gatcgaggcg caccaatccc tccttcaaag
     1501 cagcggcgcc gccgactggc agggcgcgct gaatggcggt ggagccggtc ttgtgggtgc
     1561 cgatgtggat gaagagggga ggtttcaaat cgagagcgtg aatggccgga ggtgagcggc
     1621 gggcaaggat gatgtcaccg cgtccagccc ggctcaaccg gcaaaatggc gcgcgcgttc
     1681 acgccagaac ccggcagccg cccgcgtagg agtgccatgc gcactctact gccagcctgc
     1741 attcccgctc ttttcaccgt catgctccag gccgccgctc cggtgccgct ctatatagac
     1801 ggctatgccg gaaaaataag cgtggcgcag ggggagaagg tgcctctgca tgtcagcacg
     1861 acggcggaga agtatgacat cgaggtggcg cgcctgggca gggagcgcgt ggtggtgtgg
     1921 aagcaggcgg gcgtgccggg cgcggagcat ccggtgccgg aggatgcctc ggcgcagggc
     1981 tgccgctggc ctgtgtccgt ggaggtgccg gtgggcgcgg actggcggag cggatattat
     2041 gagatcagcc tgcgcgctgc ggaccggggc gggaagtgga cggcgcgcgg cgcgcgcacc
     2101 gcgtcggacg gcgcgttctt catcggcggc caggccacgc cgggcagcgc gtcgaagatc
     2161 ctgctgcagc tcgccacgaa cacctacaac gcctacaaca actggggcgg cttcagcgtg
     2221 tatgcctaca acagccgcgg caacaaccag ggcagccgcg tttcattcga gcgtccaccg
     2281 gcctcgcagt tcagccgctg ggagctgcct ttcgtgcagt gggcggagtc tgcgggacac
     2341 accctggagt tcgctgccaa cgcggatttg gagttccatc ccgggatgct gcaaagttac
     2401 aagctcgtgc tcagcgtcgg tcacgacgaa tactggtcca caccgatgcg cgaccatttg
     2461 gaggacttca tcggcaaagg cggcaacgtc tgctttttca gtggcaacac ctgctgctgg
     2521 caggtgcgca gcgaggatga cgggcgcgcc ttcacctgct ggaagcagaa ttacttccag
     2581 gacccggtct tcaaaaccag tgaccaccgc accctgagca ctctctggag ccatcatctg
     2641 ctcaagcggc cggagactca gctcaccggc gtcggctttc tctggggcgg ctaccaccgc
     2701 agccacggcc agttcatgga tggcaaagcc gccttcaccg tccacaggcc cgcgcattgg
     2761 atcttcgaag gcaccggcct caagcgcggg gacgagtttg gcggcaagga cagcatcgtc
     2821 ggttacgagt gcgacggctg cgagctggag tggcgggacg gcctgccttt ccccacgcac
     2881 cgcgacggca gcccgcccac cttcgaagtg ctcggcacct gcgaggctcg ctggcatccc
     2941 gatgacgctg agtggtatga gaaatgggag cagggacgca ccggcgcggc ctgcatgggc
     3001 gtgcatgagc gcggcggcat cgtcttcacc gccggcacca ccgactgggc gcacggactc
     3061 tccggcggag acgcgaccgt cacgcgcatc acgcgcaact tgctggaccg gctctcgaag
     3121 tgaaatgacc tccctcgccc gcgagacggg cggcattttt ccagggttgc cccccggcga
     3181 agcggtgcta agggacggcc tgccatgcag cctcccctct ggatgaagtc ccccgccttg
     3241 cgccctctgc tccgtctttg cgccgtgctg gcgctcctcg tcgcgccagc gctcgcccag
     3301 gcgcagtccg ccgtgctttc cgcggacact ttcaaccgca aagtccacgt cgccgccggc
     3361 ggcaacaccc gccgcgccgt cggcggcctg gccaagatcg ccaccgccat ggtcacgctc
     3421 gactgggccg aagcctcccg ccagggcgtc agtgtcctcg ccaccgtgcc ggagtacgcc
     3481 ccgcgcgtgg ccgggcacaa caccctcggc ctccagccgg gtgacaaggc caccttgcgc
     3541 gacctcatct gcgccaccct catgaccagt gacgacatcg ccgccatcac gcttggcgag
     3601 ttcgtcgggc gcgaccatct ttaccgtctc ggcaggagcg gcgtgccgct cgacgagttc
     3661 gcccgccaga tgaaccagct cgccgcgcgc gagggcgcga cgaacacccg cttcaccaac
     3721 ccccacggct atgaaaacac ccgcgccgcc tcctattcca ccgcggcgga catcgcccgc
     3781 ctcgcactct acgccgtgtc ccgcccggcg ctgaagttct acaccaacca gcgcagccgc
     3841 ggcatcagcg tctatcgttc cggcggccag gtcagcatcc ccatcaccaa caccaatccg
     3901 ctgctcggcg tgtccggcat tgacggcctc aaatacacca gcacaccccg ctccggcggc
     3961 tgcatcgtcg tcaccgccga gcgcccctcc tccgtcatcc ggcaggccga cggcagcagc
     4021 ctcatctacc gccaccgcat ggtcgtcgtc gtcctcggct cgcgcgatcc cggagccgag
     4081 gcgcacgccg tcctccagca aagctggggt gcctatgacc gctggctggc cgcaggcagg
     4141 cccatcaccg acacgcgcca gcttctcaat cattactgac ccgcttggca tcctttgtgc
     4201 ggccttcacc gcgagaggag cgcccacacc ttttccaata aacaccaccc acacagacac
     4261 atgcgcatcg gcatcttgaa cagcggcggg gactgccccg gactcaacgc ggtcatccac
     4321 ggcgtcgtcg gcgccgccca caccctcggc tgggaggtcg tcggctttcg cgacggcttc
     4381 gaggggctgc tcccgcccgg cgactacatg atgctcgacc ccagcaaaac cgtcggcatc
     4441 atgaagcttg gcggcaccat cctcggcacc acgaacaagg gccacttcgt cgccaaagtc
     4501 ggcgagggca acgtggccga ggtgcccgcc gaaatcgtcg agaaggcgcg caaaatcctc
     4561 gaccagctcg agatacgcgc cctcatcgtc gtcggcggcg acggctccct caccaccggc
     4621 ctgcagcttt ataaaatggg catccccgtc atcggcgtgc ccaagaccat agacaatgac
     4681 atccaggcca ccgccatgac cttcggcttc gactccgcct gccactccgt ggtggacgcg
     4741 ctcgaccgtc tgcacaccac cgccgagagc cacaagcgcg tcatcgtcct cgaggtcatg
     4801 gggcgccacg ccggctggat cgccctttac ggcggcatct ccggcggcgc ggatgtcatc
     4861 ctgctgccgg aaatcccctt ccacatggag catgtggctg acgccatccg caagcgcgac
     4921 gcgcgcggcc tgcacagcac tctcgtcgtc gtcgccgagg gcgcgcgcat ggagtccggc
     4981 gagcttttga aaaaagagga cgtcggcggc aaaggcgagg accgcctcgg cggcatcggc
     5041 gagcatgtgg ccaagcgcat ccaggaactg acgggcaagg aaacccgcgc ctgcaccctc
     5101 ggccacctcc agcgcggcgg cgcgcccacc gcgctggacc gcatcctcgg cgtccgtttc
     5161 ggcgccaagg ccgtgcatct catcgaggag ggcaagttcg gccgcatggt cagctaccag
     5221 cactacaacg tgggcgatgt cagcatcgag gaagccgtca accagctccg cctcgtggac
     5281 cccggcagcg agatcatccg cgccggccgc agcatcggcg tctgcttcgg cgaccgcgcc
     5341 gtcggcgcgt gaaccgcgcc
//
LOCUS       DAOMYJ010000294         5342 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Prosthecobacter sp. isolate AMR_MDS_5629
            NODE_5301_length_5342_cov_20.789673, whole genome shotgun sequence.
ACCESSION   DAOMYJ010000294 DAOMYJ010000000
VERSION     DAOMYJ010000294.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867850
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prosthecobacter sp. (wastewater metagenome)
  ORGANISM  Prosthecobacter sp.
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Verrucomicrobiaceae; Prosthecobacter.
REFERENCE   1  (bases 1 to 5342)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5342)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.30
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:34:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,742
            CDSs (total)                      :: 4,684
            Genes (coding)                    :: 4,676
            CDSs (with protein)               :: 4,676
            Genes (RNA)                       :: 58
            tRNAs                             :: 55
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5342
                     /organism="Prosthecobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5301_length_5342_cov_20.789673"
                     /isolate="AMR_MDS_5629"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1965333"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..731)
                     /locus_tag="PLP58_20255"
     CDS             complement(<1..731)
                     /locus_tag="PLP58_20255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ10926.1"
                     /translation="MRRLLLPLLLAAQTASAEVEVDVEAFGQGLGGWVARKGQAAEYK
                     ISDAGYRTYQPEVSPSPDGGIYVSVRIDHRRGVFASDDHASLELSFAADGTLVSAQSS
                     LALQGRTITSELVKGGASAATNVAAPGIDHAVKVGSDLVADLSSKILREKIIEPGRVS
                     FPAAIRHNYNLLYKAVKLKDAKPAAEETAAKNPAATASPAGNDTAPSPSPPAAGPEIE
                     KPAATTPAPGSTSPAAPTPPAPVPVP"
     gene            961..1872
                     /locus_tag="PLP58_20260"
     CDS             961..1872
                     /locus_tag="PLP58_20260"
                     /inference="COORDINATES: protein motif:HMM:NF012437.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HRJ10927.1"
                     /translation="MPAFLRAHDKGLEFALEDFNLLGRSPDASIRLNDGGVSRQHATV
                     RRDGNLYWLSDLGSANGSFVNDVAVTTARALRHGDRIQIGTCVFVFETSEAAQAGDAV
                     TSSQMLHTVALPMKSIKATLLVGDLRNFTSISAQLSAEQVAAMLREWYADCERILKPR
                     GAIIDKFIGDGVFAYWAGDDVSIREQATEAARLLSSPDASESPVRKKMREEMNLEVYC
                     HIGINIGDVALGAMGRGVNTAVGEAVNVAFRIESLTRTLKVPVLVSASFISGWPGGLR
                     HFKNVGIHPVKGQPEPVEVYALIESGE"
     gene            1969..2721
                     /locus_tag="PLP58_20265"
     CDS             1969..2721
                     /locus_tag="PLP58_20265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009964664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HRJ10928.1"
                     /translation="MNQIQLSGRKAIVTGGARGIGLAIAERLLQSGASVCLWDVDAAA
                     LDTACAQLAPHGEIIGAKVDVTSADSIEAGVQKALGVFGKLDILVNNAGIAGQNAKVW
                     ELSPEDWRRVVDINLTGPFLCCRAVIPHLLANGYGRIVNIASIAGKEGNPNASHYSSS
                     KSGVIALTKSLGKELATSGVIVNCITPAVIETDIFAQMSQAHIDYMLSKIPMARFGQK
                     HEAAALTAWLCSEDCSFSTGAVFDLSGGRATY"
     gene            2769..3581
                     /gene="menB"
                     /locus_tag="PLP58_20270"
     CDS             2769..3581
                     /gene="menB"
                     /locus_tag="PLP58_20270"
                     /EC_number="4.1.3.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009963195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008935 - 1,4-dihydroxy-2-naphthoyl-CoA
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoyl-CoA synthase"
                     /protein_id="HRJ10929.1"
                     /translation="MWQTIQTFQDIKLEKTADGIGKLTINRPEVRNAFRPQTVREMLQ
                     ALDILHEDREVGVIILCGEGPLAFCSGGDQKVRGDAGYVGDDGVPRLNILDVQRKIRT
                     LPKPVVAMVAGYAIGGGHVLHVVCDLTIAADNARFGQTGPKVGSFDGGLGSSYLARIV
                     GQKKAREIWYLCRQYDAHQALEMGLVNTVVPLAELETETLKWCREMLQHSPLALRCLK
                     ASLNADCDGQMGLLDLAGNATLLYYMSDEAQEGKNAFVEKRKPDFSKFPRLP"
     gene            3612..4463
                     /gene="gluQRS"
                     /locus_tag="PLP58_20275"
     CDS             3612..4463
                     /gene="gluQRS"
                     /locus_tag="PLP58_20275"
                     /EC_number="6.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009957978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA glutamyl-Q(34) synthetase GluQRS"
                     /protein_id="HRJ10930.1"
                     /translation="MPARFAPSPTGWLHLGHAFAALFAWRQAMETDGRFLVRLEDIDT
                     TRARPEYETALFEDLAWLGVKWEEPVRRQSAHFADYQAALDQLAARELLYPCFCTRRE
                     IQEEIARAANAPHGPDGALYPGTCRDLEENERARRIASGQPYALRLHMSRALAAAPEM
                     KWQDLDHGIHTATPEIFGDVVLARKDTPASYHLAVVVDDALQGITHVTRGADLLPATH
                     LHRLLIALLGLPVPLWRHHRLITDAQGRRLAKRDDARSLRQLRAQGWTPADVRRALDL
                     PPARDSA"
     gene            4536..>5342
                     /locus_tag="PLP58_20280"
     CDS             4536..>5342
                     /locus_tag="PLP58_20280"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HRJ10931.1"
                     /translation="MLRSLFFAAIAFHTAVAEKPNIILVLADDLGWADIGCQGNTFNE
                     TPNMDRLAAQGVRFTDFYAGPVCSPTRANLQSGRDQARYGITQHIPGHKRPFAKLSDP
                     PVPLQLPLEVETVAERLGAAGYATGYFGKWHLGGEDHSPSRQGWQTAEETKGNTRPGG
                     GERTAAYLTGKAVEFIKRHQDKPFLLQVSHHAVHIPLTTTPELLAKYQRKTPMPGYPS
                     RPEYAGLLDELDQSLGRIVETVDSLGLGGKTLILFLSDNGGLEHEQSGNIV"
BASE COUNT          968 a         1918 c         1561 g          895 t
ORIGIN      
        1 ggcacgggca cgggcgcggg cggggtaggc gctgctggtg aagtactgcc aggcgcaggc
       61 gtcgtggccg cgggcttttc gatctccggc ccggcggcgg gcggcgaagg acttggcgca
      121 gtgtcattgc cagccggaga cgcggtcgca gccggattct tcgccgcagt ctcctcggca
      181 gcgggcttgg cgtccttcaa tttcacagcc ttgtagagca gattgtaatt gtggcggatg
      241 gcggcgggaa aggagacgcg ccccggctcg atgatttttt cccgcaggat tttggaggaa
      301 aggtccgcca caaggtcgct gccgaccttc accgcgtggt caatgccagg cgcggcgaca
      361 ttggtggcgg ccgaggcgcc gcctttgacc agttcgctgg tgatggtgcg cccttgaagt
      421 gccagactgc tctgggcgga gacgagagtg ccgtcggcgg cgaagctcag ctccaggctg
      481 gcatggtcgt ccgaggcgaa aaccccccgc cggtggtcaa tccgcacgga gacgtaaatg
      541 ccgccatccg gggagggcga gacctccggc tggtaggtgc ggtagccggc gtcggagatt
      601 ttatattcgg cggcctggcc cttgcgggcg acccagcctc caagcccctg accaaacgcc
      661 tcgacatcca cctccacttc agcggatgcg gtttgggcgg cgagcaggag cggcagcagc
      721 agtcgtctca taaaatgcgg gcgggttttg ggtggccggg tttaaactgg ttcctgggca
      781 ctcttattca agacgaaatg ccgcgcgccg ctgaaacccg ggcgaggcgc gcccggcagc
      841 ccgaaacaag catgattctc atgccgaagg cggttttgag cggcgttatt tgagcaaagc
      901 accggcgcag gcttgacttc cagcgccgta gttgcttcaa cagcttttta aattttatct
      961 atgcccgctt ttctcagagc ccatgacaaa ggattggaat tcgccctgga ggatttcaac
     1021 cttctaggcc gcagcccgga cgccagcatc cggctcaatg acgggggcgt ctcccgccag
     1081 cacgcaaccg tccgccggga cggcaatctt tactggctct ccgacctcgg cagcgccaac
     1141 ggcagcttcg tcaacgacgt ggccgtcacc accgcgcgcg ccctgcgcca cggggaccgc
     1201 atccagatcg gcacctgcgt ttttgttttt gaaaccagcg aggccgccca agccggcgac
     1261 gccgtcacca gctcgcagat gctgcacacc gtggccctgc cgatgaaatc catcaaggcc
     1321 accctgctcg tgggagatct gcgcaacttc accagcatct ccgcccagct cagcgccgag
     1381 caggtggccg ccatgctgcg cgagtggtac gcggactgcg agcgcatcct caagccgcgc
     1441 ggcgccatca ttgacaagtt catcggcgac ggcgtcttcg cttactgggc cggggatgat
     1501 gtctccatcc gcgagcaggc caccgaggcc gcccggctgc tcagcagccc cgatgccagc
     1561 gagtcccctg tgcgcaaaaa aatgcgcgag gaaatgaacc tcgaagtcta ttgccacatc
     1621 ggcatcaaca tcggcgacgt ggccctcgga gcgatgggcc ggggcgtcaa caccgccgtg
     1681 ggcgaggccg tgaacgtggc ttttcggatc gaaagcctca cccgcaccct caaagtgccg
     1741 gtgctcgtca gcgcctcctt catcagcggc tggcccggcg gcctccggca ctttaagaac
     1801 gtcggcatcc atccggtcaa aggccagccc gagccggtgg aggtgtacgc gctcatcgag
     1861 tccggggagt aacaaaggcc atcctccgca ttcatccgcc tcaaagcatt gacgccgcgg
     1921 gcaaactgcc gtttgcgccc ttgcgccgcc cggcttcact ggcgggccat gaatcaaatc
     1981 cagctctccg gccgcaaggc catcgtcacc ggcggcgcgc gcggcatcgg actcgccatc
     2041 gccgaacgcc ttctgcaatc cggcgcctcc gtctgcctct gggatgtgga cgccgccgct
     2101 ctcgacacag cctgcgctca gttggcgccg catggcgaga tcatcggagc aaaggtggat
     2161 gtgacctccg ccgattccat tgaggctggt gtgcaaaagg cgctcggcgt cttcggcaag
     2221 ctcgacatcc tcgtcaacaa tgccggcatc gccgggcaga acgccaaggt ctgggaactc
     2281 agcccggagg actggcggcg cgtggtggac atcaatctca ccggcccttt cctctgctgc
     2341 cgcgccgtca ttccccatct gctggccaac ggctacgggc gcatcgtcaa cattgcctcc
     2401 atcgccggca aggagggcaa tccgaacgcc tcgcattaca gctcctcaaa gtccggagtg
     2461 attgccctga ccaagtccct cggcaaggag ctcgccacct ccggagtcat cgtcaattgc
     2521 atcactcccg ccgtcattga aaccgacatc ttcgcgcaga tgtcgcaggc gcacattgac
     2581 tacatgcttt caaaaattcc catggcccgc ttcggccaga agcacgaggc cgccgcgctc
     2641 accgcctggc tgtgctcgga ggactgctct ttttccaccg gcgccgtctt cgacctctcc
     2701 ggcggccgcg ccacctactg agccgcgcag cctctcacac gcgtatcgaa atctaaaaca
     2761 cttctcccat gtggcagacc atccaaacct tccaggacat caagctcgaa aaaaccgccg
     2821 acggcatcgg caagctcacc atcaaccgcc ccgaggtgcg caacgcgttc cgcccccaga
     2881 ccgtgcgcga gatgctccag gcgctcgaca tcctgcatga ggaccgcgag gtcggcgtca
     2941 tcattctctg cggcgaggga cctctggctt tctgctccgg cggcgaccag aaagtgcgcg
     3001 gagacgccgg ctacgtcggt gatgacggcg tgccgcgcct gaacatcctc gacgtgcagc
     3061 ggaaaatccg caccctgccc aaacccgtcg tcgccatggt cgccggatac gccatcggcg
     3121 gcggccacgt tcttcatgtc gtgtgcgatc tcaccatcgc cgccgacaac gcgcgattcg
     3181 gccagaccgg ccccaaggtc ggctcttttg acggcggtct cggaagcagt tacctcgccc
     3241 gcatcgtagg tcagaagaaa gcccgcgaga tctggtatct gtgccgtcaa tatgacgcgc
     3301 accaggctct tgagatgggc ctcgtcaaca ccgtcgtccc gcttgccgaa ctcgaaaccg
     3361 agaccctgaa atggtgccgc gagatgcttc agcattcccc cctggccctg cgctgcctca
     3421 aagcctccct caacgccgac tgcgacggcc agatggggct gctagacctc gcgggcaatg
     3481 ccaccctcct ctactatatg agcgatgagg cgcaggaggg caaaaacgcc ttcgtcgaaa
     3541 agcgcaagcc cgacttctcc aagttcccgc gcctgccctg aacgccgcag ccgcaacgcc
     3601 agccaccgca catgcccgcc cgattcgcgc ccagtcccac cgggtggctg catctcggcc
     3661 atgcgtttgc cgcgctgttt gcctggcggc aggccatgga gactgacggg cgcttcctcg
     3721 tgcggctgga ggacattgac accacccgcg cgcgtcctga atacgagacg gcgctgttcg
     3781 aggatctggc ctggctcggc gtgaaatggg aagagcctgt gcgccgccaa tccgcgcact
     3841 tcgccgatta ccaggccgcg ctcgaccagc tcgccgcgcg cgaactgctc tatccctgct
     3901 tctgcacgcg gcgggaaatt caggaggaga tcgcccgcgc cgcgaacgcc ccgcacgggc
     3961 cggatggcgc gctttatcca ggcacctgcc gcgacctgga ggaaaacgag cgcgcgcgcc
     4021 gcatcgcctc cggccagccc tacgcgctgc gcctgcacat gagccgcgcg ctggccgccg
     4081 ccccggaaat gaaatggcag gacctggatc acggcatcca cactgcaacg ccggagatct
     4141 tcggggacgt tgtcctggcg cgcaaggaca ctcccgccag ctaccacctc gccgtcgtcg
     4201 tggatgacgc gctgcagggc atcacccacg tcacgcgcgg agccgatctt ctgcccgcca
     4261 cgcacctgca ccgcctgctc atcgccctgc tgggactccc cgtgcccctc tggcgccacc
     4321 accgcctcat cacagacgcc cagggccgcc gcctcgccaa acgcgacgac gcgcgctccc
     4381 tccgccagct ccgcgcccag ggctggacac ccgccgatgt ccgccgcgcc ctggatttgc
     4441 cgcctgcgcg agattccgca tgacgattcg cgcaattccg gcgaccggcg cgcggttctt
     4501 gagcctgcca gttttcccac cgccctcacc cttccatgct ccgctccctc tttttcgccg
     4561 ccatcgcctt tcacaccgcc gttgctgaaa agcccaacat catcctggtc ctcgccgacg
     4621 acctcggctg ggcggacatc ggctgccagg gcaacacctt caatgagacg ccgaacatgg
     4681 accgcctggc cgcgcaaggc gtgcgcttca cggatttcta cgcgggaccg gtctgctccc
     4741 ccacccgcgc caatctgcaa agcggccgcg accaggcccg ctacggcatc acccagcaca
     4801 tccccggcca caagcgcccc ttcgccaaac tcagtgaccc gcccgtgccg ctgcaactgc
     4861 ccctggaggt cgagaccgtc gccgaacgcc ttggcgcggc aggatacgcc accggttatt
     4921 tcggcaaatg gcatctcggc ggggaggacc acagtccctc gcggcaaggc tggcagaccg
     4981 ccgaggaaac caaaggcaac acccggcccg gcggcggcga acgcaccgcc gcatacctca
     5041 ccggcaaagc cgtcgagttc atcaagcgtc accaagacaa gccctttctc ctccaggtct
     5101 cccaccacgc cgtccacata cccctcacca ccacgcctga gctgctggcc aaataccagc
     5161 gaaaaacccc catgcccggc taccccagcc gccccgaata cgccggcctc cttgatgaac
     5221 tggaccaaag tttgggccgc atcgttgaga ccgttgacag cctcggcctc ggcggcaaaa
     5281 cgctcatcct tttcctctcc gacaacggcg gcctcgagca cgagcaaagc ggcaacatcg
     5341 tc
//
LOCUS       DAOMYJ010000295         5335 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Prosthecobacter sp. isolate AMR_MDS_5629
            NODE_5313_length_5335_cov_19.257008, whole genome shotgun sequence.
ACCESSION   DAOMYJ010000295 DAOMYJ010000000
VERSION     DAOMYJ010000295.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867850
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prosthecobacter sp. (wastewater metagenome)
  ORGANISM  Prosthecobacter sp.
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Verrucomicrobiaceae; Prosthecobacter.
REFERENCE   1  (bases 1 to 5335)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5335)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.30
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:34:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,742
            CDSs (total)                      :: 4,684
            Genes (coding)                    :: 4,676
            CDSs (with protein)               :: 4,676
            Genes (RNA)                       :: 58
            tRNAs                             :: 55
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5335
                     /organism="Prosthecobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5313_length_5335_cov_19.257008"
                     /isolate="AMR_MDS_5629"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1965333"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1029
                     /locus_tag="PLP58_20285"
     CDS             <1..1029
                     /locus_tag="PLP58_20285"
                     /inference="COORDINATES: protein motif:HMM:TIGR01494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HRJ10932.1"
                     /translation="AEAIEKLAALNTLAVDKTGTLTLGRPEVTEILLAPHTLLSRDDA
                     LRHAAALERHSEHPLAHAILQAAEKKQLALPDVPDFQAAPAAGVSGRVEGRAVVVGKT
                     DFLRAHGVENLGGLETLATPHQGEGKSAIFLAVDRQAAAVIVVSDPIKPTTPEALARL
                     RALGISVVMLTGDNERTAQRIATTLGIGRFHAGVTPQEKHAFITAMKRPGAVVGMAGD
                     GINDAPALAEADVGIAMGTGTDIAMETAPVTLVRGDLRGIVHAIQLGRAMMRNIRQNL
                     LFAFLYNALGIPVAAGILYPSLGVLLSPMLAGAAMSLSSVSVIANALRLHRFRGGERR
                     LVNEMYFQ"
     gene            complement(1109..1837)
                     /locus_tag="PLP58_20290"
     CDS             complement(1109..1837)
                     /locus_tag="PLP58_20290"
                     /inference="COORDINATES: protein motif:HMM:NF024258.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HRJ10933.1"
                     /translation="MDDALAVLSDLHGNLHALQAVLADVEAQGIRELVCLGDVVGYGG
                     SPAECVEVLRAAGIRCLRGNHDAMVASSSGMDGLTLQTRLAVEWTARVLSVEQRAWLA
                     ELPMTLEGEDWEAVHACLHRADDWPYVLTADMAALHFTHQSKPLCFIGHTHRPAMWVE
                     GEALGVDITSIENLRAGKKCLVNAGAVGQPRDRDSRACYAVYRRGQRDIWWRRVAYDV
                     AAAQKAIHEAGLPPYFAQRLELGR"
     gene            1920..2498
                     /locus_tag="PLP58_20295"
     CDS             1920..2498
                     /locus_tag="PLP58_20295"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HRJ10934.1"
                     /translation="MTAEQRFTLHLPLAAQIAAGYANIPGVSLDDIRSVADAALHRAS
                     LAFDAEKGDFKPYAARAIRNALNDLHQKQARQTRLLVGEADLPQADAASTSPSHRLPD
                     AGADVVLQIREAESRRALEQALAALPVRGRQILALLAQGCSYSEMGERLGVSKQAASK
                     AAAKAMQTLREELARQGFHGLDTQGLLKTDPP"
     gene            2569..3855
                     /locus_tag="PLP58_20300"
     CDS             2569..3855
                     /locus_tag="PLP58_20300"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0051219 - phosphoprotein binding [Evidence
                     IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing serine/threonine-protein
                     kinase"
                     /protein_id="HRJ10935.1"
                     /translation="MSAKVQFRITEGAARGKVFTFAAHDTFFLGRHPDCHVHLADDTF
                     VSRHHFILEVCPPQVCVRDLGSRNGTYVNGRKIGGRKEGETPEEGARRAYPQVNLKHG
                     DRIQVGDTALEVAVSQPAAAPGQAAAAAPLPAFDELDPKHLADLLFKAAQNKKAPRLQ
                     IPGYEIEKELGRGGFGVVFRARRLADQLPVAIKVLLPRVAVLPAEMEKFLREMAVTAQ
                     LRHHNIVKLYDQGHSEGIPWFIMEYCEGGSLWDHIMRHGGKLPLEAARPIMLDALEGL
                     THAHASGIVHRDLKPQNILMDRGVARISDFGLAKNYEQAGFSGLSVTGNYAGTPLFMP
                     REQLINFKHLKPVSDVWSIAATFYFMLTGAFPYPFTKNRDPIDVILNEPIVPISARKC
                     SIPAELARVIDQALALQLSNRFPTAGEMLSAIQKIR"
     gene            3852..>5335
                     /locus_tag="PLP58_20305"
     CDS             3852..>5335
                     /locus_tag="PLP58_20305"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HRJ10936.1"
                     /translation="MNGNEQEIDPLEWQVGDTILETYKIKRKIESGGMGIVYGASHLH
                     WNIDIAIKSPRASLFDTDSNRDNFKREAETWISLGLHPHIVECFYVQRIGGTPLIIAE
                     FVNGGTLAEWIQNGKFSEGSDVDRLRRILDIAIQIAWGLNFAHKKSLVHQDVKPGNIL
                     MTENGVAKVSDFGLSSALKFTNASSSPNYRQGQSILVPGAGFLTREYASPEQFSGEML
                     TRRSDIWSWAVLVLEMLKGECDWQDGRVAPEVLTCFYGSALKSDPLGLLLEKCLQKLP
                     ADRPNDFGAVADELKAIWKATGAGEYFRTLPEGDLMEIASINNRAVSMLELDHQSDAD
                     TVKSGMTLFDDLLKRFPLNKTVRLNCALFRWLHCGAPIRDLDREIEMILQLPGPPVAP
                     DWLIRVELAKLDTRRALSIPHRSAKSDEAISVLHCALKERFQILFENATKNSESSSCQ
                     SQRSSLGSTGLSESKQDSTDSEQNAATCKAFLEVQRYPQAKVAVR"
BASE COUNT         1079 a         1754 c         1523 g          979 t
ORIGIN      
        1 gcggaggcca tcgagaaact ggccgcgctg aacacgctgg cggtggacaa gaccggcacg
       61 ctgacgctgg gccgaccgga ggtgacggag atcctgcttg caccccacac cctgctgagc
      121 cgcgacgacg cgctgcgcca tgcggcggcg ctggagcgcc acagcgagca tccgctggcc
      181 catgccatcc tccaggcggc ggagaaaaag cagctggccc tgcccgatgt gcccgatttt
      241 caggccgcgc ccgcggcagg tgtcagcggc cgggtggagg gccgggcggt ggtggtcggc
      301 aagacggact ttctgcgcgc gcacggtgtg gaaaacctcg gcggcctgga aaccctggcg
      361 actccccatc aaggcgaagg caagtcggcc atctttcttg ccgtggaccg gcaggcagcg
      421 gcggtcatcg tcgtgagcga ccccatcaag cccaccacgc ccgaggcgct ggcccggctg
      481 cgcgcgctgg gcatctccgt ggtcatgctg acgggcgaca acgaacgcac ggcgcagcgc
      541 atcgccacga cgctgggcat cgggcgcttc catgccgggg tcacgccgca ggagaagcac
      601 gccttcatca ccgcgatgaa gcgccccggc gctgtcgtcg gcatggcggg cgatggcatc
      661 aatgacgcgc ctgccctggc cgaggccgat gtcggcatcg ccatgggcac gggcacggac
      721 atcgccatgg agaccgcgcc ggtcacgctg gtgcgcggcg acctgcgcgg catcgtccac
      781 gccatccagc tcggccgcgc gatgatgcgg aacatccggc agaacctgct gtttgccttc
      841 ctctacaacg ccctgggcat ccccgtcgcc gccggcatcc tgtaccccag cctgggcgtg
      901 ctgctcagcc cgatgctcgc cggcgccgcg atgagcctca gctccgtctc cgtcatcgcc
      961 aacgccctgc ggctgcaccg gttcaggggg ggtgagagga ggctggtgaa cgagatgtat
     1021 tttcaatgag ttgcacacgg aagccgtctc ccgtttcccc aactttgcga cgaccctctt
     1081 ttcctcagcc cctcgcgaat cagccacctc atctccccag ctccagccgt tgcgcaaagt
     1141 agggcggcag ccccgcctca tggatcgcct tctgcgcagc cgccacatcg taggccacgc
     1201 gccgccacca gatgtcccgc tgcccgcgcc gatacaccgc gtagcacgcg cgtgagtcgc
     1261 ggtcgcgcgg ctggcccaca gctcccgcgt tcaccaggca tttcttgccc gcgcgcaggt
     1321 tctcaatgct cgtgatgtcc acccccagcg cctccccctc cacccacatc gcaggccgat
     1381 gcgtgtgccc gatgaagcac agcggcttgc tttgatgcgt gaaatgcagc gccgccatgt
     1441 ccgccgtcag cacatagggc cagtcatccg cccggtgcag gcaggcatgg acggcctccc
     1501 agtcttcccc ctccagcgtc atcggcagct ccgccagcca ggcccgctgc tccacgctca
     1561 gcacgcgcgc cgtccactcc accgccagcc gcgtttgcag cgtcaacccg tccatgccag
     1621 acgacgaggc caccatcgca tcatgattgc cccgcaggca gcggatgccc gccgcgcgca
     1681 gcacctccac gcactccgcc gggctgccac catacccgac cacatccccc aggcagacca
     1741 actcccggat tccctgcgcc tccacatccg ccagcaccgc ctgcaaagcg tgcaggttcc
     1801 cgtgaagatc gctcaaaacc gccagggcat catccatgcg ctcaactacg gcccggaaga
     1861 aaaaacgtca accccgcgcc cttgcctcgc tgcccatcct gggtgcatga tgcggcacga
     1921 tgaccgccga gcagcgcttc acactccacc tgcctctggc cgcgcagatc gcggcgggct
     1981 atgccaacat ccccggcgtc tccctggatg acatccgctc cgtcgccgac gcggccctgc
     2041 atcgggcgtc tctggctttt gatgctgaaa aaggggactt caagccctac gcagcacgcg
     2101 ctatccgcaa tgccttgaat gatctgcatc aaaagcaggc gcgccagacc cgcctgctcg
     2161 tcggcgaggc cgatctcccc caggcagacg ccgcctccac cagccccagt catcgcctgc
     2221 ctgatgccgg agcggatgtg gtgctgcaaa tccgggaggc cgaaagccgt cgcgccctgg
     2281 agcaggcgct cgccgctctg cccgtgcgcg gcaggcagat ccttgccttg ctggcccagg
     2341 gttgcagcta ctccgagatg ggcgagcgcc tcggcgtcag caagcaggcc gccagcaaag
     2401 ccgccgccaa agccatgcag acgctccggg aagagcttgc ccgccagggt tttcatggcc
     2461 tcgacactca gggcttgctg aaaacggacc cgccgtagtt caatgcagtc tgcctgcagg
     2521 ggagcggcgg gttgacgttt tttcggaaag tccgtagttg ccctcattat gagcgcaaag
     2581 gtccagttcc gaatcactga gggtgccgcc aggggcaaag tcttcacctt cgccgcgcat
     2641 gacacctttt tcctcggcag gcatcccgac tgccatgtgc atctggcgga cgacaccttt
     2701 gtctcccgcc accacttcat cctggaggtc tgcccgccgc aggtctgcgt gcgcgacctc
     2761 ggcagtcgca acggcaccta tgtgaacggg cgcaagattg gcgggcgcaa ggaaggcgaa
     2821 accccggagg agggcgcgcg ccgggcctat ccccaggtga atctcaaaca tggcgaccgc
     2881 atccaggtgg gcgacaccgc cctggaggtg gccgtgagcc agcccgcggc cgccccaggt
     2941 caggctgcag ccgcagcgcc gttgcccgcc tttgatgagc tggaccccaa gcacctggcc
     3001 gacctgctgt tcaaggccgc ccaaaacaag aaagcgcccc gtcttcagat ccccggctac
     3061 gagattgaaa aggagcttgg ccgcggcggc tttggagtcg tcttccgcgc gcggcgtctt
     3121 gccgatcagc ttcccgtcgc catcaaggtg ctgctgccca gggtggcggt gcttccggcg
     3181 gagatggaaa agttccttcg cgagatggcc gtcaccgccc agctccgcca tcacaatatc
     3241 gtcaagctct acgaccaggg ccacagcgag ggcatcccct ggttcatcat ggagtactgc
     3301 gagggcggca gcctctggga tcacatcatg cggcacggcg gcaagctgcc gcttgaagcc
     3361 gcccgaccca tcatgctgga tgcgctggaa ggcctgaccc atgcccacgc ctcaggcatc
     3421 gttcatcgcg acctcaagcc gcagaacatc ctcatggacc gtggcgtggc ccgcatctct
     3481 gacttcgggc tcgccaaaaa ctacgagcag gccggcttca gcggcctcag cgtcaccggc
     3541 aactacgccg gcaccccgct cttcatgccg cgcgagcagc tcatcaactt caagcacctc
     3601 aaacccgtct ccgacgtctg gagcatcgcc gccaccttct acttcatgct caccggcgcg
     3661 ttcccgtatc ccttcaccaa aaaccgcgac ccaattgatg tgattctcaa tgagcccatc
     3721 gtgcctatct ctgcgaggaa atgttcgatc ccggctgaac tggcgcgcgt cattgaccag
     3781 gcgctggcac tccaactgtc aaaccgattt cccactgcgg gagagatgct ttccgcaatt
     3841 caaaaaattc gatgaacggc aacgaacaag agattgaccc attggaatgg caggtcggcg
     3901 atacgatttt ggaaacctac aagatcaagc ggaaaatcga atcaggcgga atgggaatag
     3961 tgtatggagc ttcccacttg cattggaaca ttgacattgc aatcaaatcg ccgcgcgcat
     4021 cgcttttcga tactgattct aatcgcgata acttcaaacg agaagccgaa acttggatct
     4081 cactaggact tcatcctcac atcgttgagt gcttttatgt gcaaagaatc ggcggcacgc
     4141 ctctaatcat cgcagagttt gtcaatgggg gcactttggc cgaatggatt caaaacggaa
     4201 agttcagtga aggatcggac gtggacaggc tgcgcagaat actggacata gcgattcaaa
     4261 tcgcttgggg gctcaatttc gcgcataaaa aaagcttggt tcatcaggat gtgaaaccgg
     4321 gcaacattct aatgactgag aacggtgttg ctaaggtcag cgattttggt ctttcaagtg
     4381 ctctcaagtt caccaatgcg tcttcaagtc cgaattaccg acaggggcaa agcatcttgg
     4441 ttccgggtgc tggattcctc acccgcgagt acgcctcccc ggagcagttt agtggggaga
     4501 tgttgacacg ccgctctgat atttggagtt gggcggttct ggttctggag atgctcaaag
     4561 gtgaatgtga ttggcaggac gggcgagttg cgccagaagt gctaacttgt ttttatggca
     4621 gtgcgctaaa gtctgaccca cttggcctat tacttgaaaa atgccttcaa aagcttccag
     4681 cagaccgacc aaatgacttt ggggctgtcg ctgacgaatt gaaggcaatt tggaaagcca
     4741 caggagcagg cgaatacttc cggaccctgc ctgaaggtga cttgatggaa attgcgtcca
     4801 taaataaccg agcggtttcc atgttggaac tggaccacca gtctgatgca gacacagtca
     4861 agagcgggat gaccttattc gacgatttac tgaaacggtt tccgctaaac aaaacggttc
     4921 gattgaactg tgccttgttc cgctggcttc attgcggggc tcccatcagg gacctagacc
     4981 gggagattga aatgatactg caactaccgg gtccgccagt cgcccccgat tggctcatca
     5041 gagtggaact cgccaaattg gacacacgtc gcgcactttc catcccacat cgctctgcta
     5101 aaagtgacga ggcaatctca gttcttcact gcgcattgaa ggaacgtttt caaatcttat
     5161 ttgaaaatgc tacaaaaaac agtgagagca gctcatgtca aagccagcgt tccagtttgg
     5221 gctctactgg gctcagtgaa tctaagcagg actcgacgga ttcagaacaa aacgcagcta
     5281 cgtgcaaagc attcttggag gtgcaaagat atcctcaagc caaagtggcc gtccg
//
LOCUS       DAOMYJ010000296         5327 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Prosthecobacter sp. isolate AMR_MDS_5629
            NODE_5326_length_5327_cov_19.952580, whole genome shotgun sequence.
ACCESSION   DAOMYJ010000296 DAOMYJ010000000
VERSION     DAOMYJ010000296.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867850
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prosthecobacter sp. (wastewater metagenome)
  ORGANISM  Prosthecobacter sp.
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Verrucomicrobiaceae; Prosthecobacter.
REFERENCE   1  (bases 1 to 5327)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5327)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.30
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:34:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,742
            CDSs (total)                      :: 4,684
            Genes (coding)                    :: 4,676
            CDSs (with protein)               :: 4,676
            Genes (RNA)                       :: 58
            tRNAs                             :: 55
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5327
                     /organism="Prosthecobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5326_length_5327_cov_19.952580"
                     /isolate="AMR_MDS_5629"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1965333"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1477)
                     /locus_tag="PLP58_20310"
     CDS             complement(<1..1477)
                     /locus_tag="PLP58_20310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ10937.1"
                     /translation="MLCLPLLLAGCGTLPKTNIPRTLAQERHTVTQGFQARVPRHQGA
                     EFLTFEELKSLAREPKPGGELEAKLERFFSRPIISNEAWYSGKRPTRPSNDTLGEHLS
                     IATWNIEKSIHAHEAARVLGSEAAFEALIDPAIAPPGSKLRADLLRQRQRLAGADVIL
                     LQEMDIGIGRSGYHDSARDIARALGMNYAYAPKQLEIDPVLLGLESIPDGRGGSVRHQ
                     PDPARYKGVFGKAVLSRYPIKSARCFQLQTQPYDWHEGERKDTDLLEDSRRFAAEIMF
                     ENKMVREMKVGGRVFFQVDLEVPGLPGDTLTLIHNHLEIKARPQDREAQMAEILSHIR
                     HIPHTVVMGGDHNSSRADLSPTSATRVIRRTTLDSETWLGVGMNALMSAPMLVNSGRI
                     LLNNVKNLHSPLAPHIPVILPNRTRAMFEQIEDFRFRDGGRFDFRGDRSRSINQSSAT
                     LANSNEKAIKGHTPSFSVRRPIGPFGKSRLDWLFVKAPPVAQ"
     gene            complement(1591..2214)
                     /gene="hisH"
                     /locus_tag="PLP58_20315"
     CDS             complement(1591..2214)
                     /gene="hisH"
                     /locus_tag="PLP58_20315"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009962116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisH"
                     /protein_id="HRJ10938.1"
                     /translation="MNLGVLDYGAGNLRSVLNAFTAIGSRARLVTAPAEFEGVDVLVF
                     PGQGAFGDSVRILKERRLWEPLREWLRAQRPYLGICLGYQLLFESSEESPGVEGLCAA
                     PGRVRKFQAGEGLKIPHMGWNEVRWEPRAAAWWQGLPNPAHLYFVHSYYPEVADDSLA
                     LCRTGYGVDFVSGIARKNLVAVQFHPEKSQDTGLKLLGGALRVLAEA"
     gene            complement(2211..2813)
                     /gene="hisB"
                     /locus_tag="PLP58_20320"
     CDS             complement(2211..2813)
                     /gene="hisB"
                     /locus_tag="PLP58_20320"
                     /EC_number="4.2.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009962117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004424 - imidazoleglycerol-phosphate
                     dehydratase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazoleglycerol-phosphate dehydratase HisB"
                     /protein_id="HRJ10939.1"
                     /translation="MQQPRSAALSRKTAETDIQIELVVDGAGVSRIDTGVPFFDHMLT
                     LFAKHGLFDLKVKVTGDIEVDYHHTVEDTGIVLGQCFREALGDKRGITRYGFFLLPMD
                     ETLAEVALDLSGRAFLSYHAPEGVEAIGGVNRFSYQLVEEFLRAFASSLLATLHVEIR
                     RGRDAHHMAEAIFKGLARALDAATRIDPRVTGIPSTKDVL"
     gene            complement(2915..3607)
                     /locus_tag="PLP58_20325"
     CDS             complement(2915..3607)
                     /locus_tag="PLP58_20325"
                     /inference="COORDINATES: protein motif:HMM:NF012360.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ10940.1"
                     /translation="MTRRATLMTLSLPLPRLAAYIARQINTFFPDGEDITDGQVRPLL
                     EAAAAELGEGLSRFHNAYFWRGGQVHLDHLHGDQYLLLLYRLGHHAALAGPARRGLMD
                     KCYLLNKALHGLDIYPQVSLPAACWLAHPVGTVLGRADYRGPLMTMQGCTIGNKSGGY
                     PVIGSNVVLCARTTILGACEIGDRVCFGAGSLVLDEKIPSDSTVVGRSPNLRVIPRRA
                     NLISRVFRDVAE"
     gene            complement(3608..4909)
                     /locus_tag="PLP58_20330"
     CDS             complement(3608..4909)
                     /locus_tag="PLP58_20330"
                     /inference="COORDINATES: protein motif:HMM:NF027579.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4910 domain-containing protein"
                     /protein_id="HRJ10941.1"
                     /translation="MADSTEDTHSMHSLLRRLFPICRSITGEGVRETLRILRGHMPGL
                     RIHEVPTGTPCFDWEVPPEWNITAATLTGPDGQTLADFKDHNLHVVSYSEPVDLELSL
                     DELQPHLHSLPAQPDAIPYVTSYYRRDWGFCLADRVRRSLQPGRYRAIIRSTLENGSL
                     TYGELVLPGETTDEILVSTYICHPSMANNELSGPVVATWLAKHLAARPRRHTLRFIFI
                     PETIGSLVYLSRHLDHLKKHVKAGLVLSCVGDERAWSWLSSRLGGTLADKAAAHVMRH
                     LHPEHHAYSWLHRGSDERQFCWPGVDLPVCSFMRSKYATYPEYHTSLDNPGLVTEAGL
                     RGSFDTARVFIECIERNQTYRATTLGEPRLGKRDLYPAASTIAPEAARAVTSARTLVD
                     ILAYCDGAHDLLDVAERLGKPVWELFDGVQTLMDHHLLRPA"
BASE COUNT          838 a         1478 c         2100 g          911 t
ORIGIN      
        1 gctgggcgac gggcggggct ttgacgaaga gccagtcgag gcggcttttg ccgaaggggc
       61 cgatgggacg gcggacgctg aagctggggg tgtggccttt gatggctttt tcgttggagt
      121 tggcgagcgt ggcggaggac tggttgatgg agcgggagcg gtcgccgcgg aagtcgaagc
      181 ggccgccgtc gcggaagcgg aagtcctcga tctgctcgaa catggcgcgg gtgcggttgg
      241 ggaggatgac ggggatgtgg ggggcgaggg ggctgtggag gtttttgacg ttgttgagga
      301 ggatgcggcc ggagttgacg agcatggggg cggacatgag ggcgttcatg ccgacgccga
      361 gccaggtctc ggagtcaagg gtggtgcggc ggatgacgcg ggtggcggag gtggggctga
      421 ggtcggcgcg ggaggagttg tgatcgccgc ccatgacgac ggtgtggggg atgtggcgga
      481 tgtgggagag gatctcggcc atctgcgcct cgcggtcctg ggggcgggct ttgatctcga
      541 ggtggttgtg gatgagggtg agggtgtcgc cggggaggcc ggggacttcg aggtccacct
      601 ggaagaagac acgcccgccg actttcatct cgcggaccat tttgttttca aacatgatct
      661 cggcggcgaa gcggcgggag tcctccagga ggtcggtgtc tttgcgctcg ccctcatgcc
      721 agtcgtaggg ctgggtctgg agctggaagc agcgggcgct tttgatgggg tagcgcgaga
      781 gcacggcctt gccgaagacg cctttgtagc gggcggggtc gggctggtgg cggacgctgc
      841 ctccgcggcc gtcggggatg ctctcgaggc cgaggaggac ggggtctatt tcgagctgct
      901 tgggggcgta ggcgtagttc atgccgagag cgcgggcgat gtcgcgcgcg gagtcgtggt
      961 agcctgagcg gccgatgccg atgtccatct cctggagcag gatgacatcg gcgccggcga
     1021 ggcgctggcg ctggcggagg agatcggcgc gcaatttgct gccgggcggg gcgatggcgg
     1081 ggtctataag cgcctcgaaa gcggcctcgg agcctaggac gcgggcggcc tcgtgggcgt
     1141 ggatggactt ttcgatgttc caggtggcga tgctgaggtg ctcgccgagg gtgtcattgc
     1201 tggggcgggt ggggcgcttg ccggagtacc aggcctcgtt ggagatgatg gggcggctga
     1261 agaagcgctc cagctttgcc tccagctcgc cgccgggctt gggctcgcgc gcgagacttt
     1321 tcagctcctc gaaggtgagg aactcggcgc cctgatggcg aggcacacgc gcctggaaac
     1381 cctgcgtcac ggtgtgccgc tcctgcgcca gggtgcgcgg gatgttcgtc ttcggcagtg
     1441 tgccgcagcc cgccagcagc aggggcaggc agagcatggg tgggaggcgg tgaaaaacgg
     1501 gcatgcggcg tcgtgcggga tgccaagcat tcgccccgcg gtcaagcctg ggatgccgcg
     1561 aagtgcgggc gcgcggcggc cccgccccgg tcatgcctcc gccagcacgc gcaacgcgcc
     1621 gcccaggagc ttgagtcctg tgtcctgact tttctccggg tggaactgca cggcgacgag
     1681 gtttttcctt gcgatgccgg agacgaagtc caccccgtag ccggtgcggc agagcgcgag
     1741 cgagtcatcc gccacctcgg ggtagtagct gtggacgaag taaaggtgcg ccggatttgg
     1801 cagcccctgc caccaggcgg cggcgcgcgg ctcccagcgg acctcgttcc aacccatgtg
     1861 agggattttc agtccttctc ccgcctgaaa cttgcgcacc cgtccgggcg cggcgcacag
     1921 gccctccaca ccgggggact cctcgctgga ctcgaagagc aactggtagc ccaggcagat
     1981 gccgaggtag ggacgctgcg cgcgcagcca ctcgcgcagc ggctcccaga gccggcgctc
     2041 ctttaaaatg cgcacgctgt cgccaaaggc cccctgtccg ggaaacacga gcacatccac
     2101 gccctcgaac tccgccggcg cggtgaccag gcgggcccgg ctgccgatgg cggtgaaggc
     2161 gttgagcacg ctgcgcaggt tgcccgcgcc gtagtccagc acacccaggt tcataggacg
     2221 tccttcgtgc tcgggatgcc cgtcacgcgc gggtcaatgc gcgtggctgc gtcaagtgcc
     2281 cgtgccaggc ccttgaaaat ggcctccgcc atgtggtgcg cgtcacgacc gcggcggatc
     2341 tccacatgca gggtcgccag caggctggag gcaaacgcgc gcaggaactc ctccacgagc
     2401 tggtaactga agcggttcac cccgccgatg gcctccaccc cctccggcgc gtggtagctg
     2461 agaaaagcgc gcccgctcag gtccagcgcc acctcggcca gagtctcatc catgggcagg
     2521 agaaaaaagc cgtagcgtgt gatgccgcgc ttgtctccca gcgcctcgcg gaagcactga
     2581 cccagcacga tgccggtgtc ctccaccgtg tgatggtagt ccacctcgat gtcgcccgtg
     2641 accttcactt tcaggtcgaa aaggccgtgc ttggcgaaga gggtgagcat gtggtcgaag
     2701 aacggcacgc cggtgtcaat ccgggacaca ccggcgccgt ccaccaccag ctcgatctgg
     2761 atgtcggtct cggcggtctt gcgggatagg gctgcggagc ggggttgttg catgaagggt
     2821 ctcactaaag ccccatcccg cgcgcgggtc aatgcgccgc tgctgcccga tccccgtcat
     2881 gccgctgtcc gatgcggccg cgcgcgcggc gcggtcactc cgccacgtcg cggaagacgc
     2941 ggctgatgag gttggcgcgg cgggggatga cgcgcaggtt cgggctgcgg ccgacgacgg
     3001 tggagtcgga ggggattttc tcgtcgagca cgaggctgcc cgcgccgaag cagacgcggt
     3061 cgccgatctc gcaggcgccg aggatggtgg tgcgggcgca gaggacgaca ttgctgccga
     3121 tgacggggta gccgccgctt ttgttgccga tggtgcagcc ctgcatggtc atgagcgggc
     3181 cgcggtagtc ggcgcgtccg agaaccgtgc ccacggggtg ggccagccag caggcggcgg
     3241 gcagggagac ctgggggtag atgtcgaggc cgtggagggc tttgttgagc aggtagcact
     3301 tgtccatgag gccgcgccgc gcggggccgg ccagggcggc gtggtggccg aggcggtaga
     3361 ggaggaggag gtattggtcg ccgtggaggt gatccaggtg gacctggccg ccgcgccaga
     3421 agtaggcgtt gtgaaagcgg gagaggccct cgccgagttc ggcggcggcg gcttcaagga
     3481 gtggccgcac ctggccgtcg gtgatgtcct cgccgtcggg gaagaaggtg ttgatctggc
     3541 gggcgatgta ggcggcgagg cgcggcaggg ggagggagag ggtcatgagc gtggcgcggc
     3601 gggtcattca ggcgggacgc agaagatggt gatccatcag ggtctggacg ccatcgaaga
     3661 gttcccacac gggtttccca agccgctcgg cgacatcgag aagatcgtgc gcgccgtcgc
     3721 agtaggcgag gatgtccacg agggtgcggg cggaggtgac agcgcgggcg gcttcggggg
     3781 caatggtgct ggcggcgggg taaaggtcgc gcttgccgag gcggggctcg ccaagggtgg
     3841 tggcgcggta ggtttgattg cgctcgatgc actcgatgaa gacgcgcgcg gtgtcgaagg
     3901 agccgcgcag tccggcttcg gtgacgaggc cgggattgtc cagggaggtg tggtattcgg
     3961 ggtaggtggc gtatttgctg cgcatgaagg agcagactgg caggtccacg ccgggccagc
     4021 aaaactggcg ctcgtcactg ccgcggtgca gccaggagta ggcatggtgc tctggatgca
     4081 ggtggcgcat gacatgggcg gcggctttgt cggccagggt gccgccaagg cgggaggaga
     4141 gccaggacca ggcgcgctcg tctccgacgc aggagagcac aaggccggct ttgacatgct
     4201 ttttcaaatg gtcgagatgg cgggagagat agacgaggct gccaatcgtt tccgggatga
     4261 agatgaagcg cagggtgtgg cggcgcgggc gggcggcgag gtgcttcgcc agccaggtgg
     4321 cgacgacggg gccggagagt tcgttgttgg ccatcgaggg gtggcaaatg taggtggaga
     4381 cgaggatttc atccgtcgtc tcgccaggca ggaccagttc gccgtaggtg aggctgccgt
     4441 tttccaatgt ggagcggatg atggcacggt aacgtcccgg ctggagggag cggcggacgc
     4501 ggtccgcgag gcagaagccc cagtcgcgcc ggtagtagct ggtgacatag gggatggcgt
     4561 cgggctgcgc gggcagggag tggagatggg gctggagttc gtcgaggctg agttcgagat
     4621 caaccggctc tgagtagctg acgacgtgga ggttgtggtc tttgaagtcc gcgagggtct
     4681 gcccgtccgg gccggtgagg gtggcggcgg tgatgttcca ctccggcggc acctcccagt
     4741 caaagcaggg cgtgcctgtg gggacctcgt ggattcgcag gccgggcatg tgcccgcgca
     4801 ggatgcgcag ggtctcgcgc acgccctcgc cggtgatgct gcggcagatg gggaaaagcc
     4861 gacgcagcag gctgtgcatg gagtgggtgt cttcggtgct gtctgccatg ggaaaagaaa
     4921 cgaggcatgc ccatgaagcc gcgccagcaa gaccgcgcaa ggggaaggat gcgtccggag
     4981 cgcgtcagcg cggggtgctg cggagcgttc ccgacggcat caactgcatg gtaaacgctg
     5041 tgcctcttgg gtttagccgc tggcgatgaa gatggcggct tcagtgacga tggatggcgg
     5101 tgtgttgaat gaggctctca tggcacccca tcatagcaaa ctccgtttta cccagggaat
     5161 gccgctggaa cccttgattt tccgtgcttc atgtcgcctg acaccgtttc cccgccgtcg
     5221 aaaaagctgc gccagttcag gccgtgcgcg gtctcccagg cgtcgggctt gccgtcgtgg
     5281 tcggcgtctc cggcgggggc gggcgctggg cggcgagctt tgcctca
//
LOCUS       DAOMYJ010000297         5319 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Prosthecobacter sp. isolate AMR_MDS_5629
            NODE_5333_length_5319_cov_18.784954, whole genome shotgun sequence.
ACCESSION   DAOMYJ010000297 DAOMYJ010000000
VERSION     DAOMYJ010000297.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867850
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prosthecobacter sp. (wastewater metagenome)
  ORGANISM  Prosthecobacter sp.
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Verrucomicrobiaceae; Prosthecobacter.
REFERENCE   1  (bases 1 to 5319)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5319)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.30
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:34:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,742
            CDSs (total)                      :: 4,684
            Genes (coding)                    :: 4,676
            CDSs (with protein)               :: 4,676
            Genes (RNA)                       :: 58
            tRNAs                             :: 55
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5319
                     /organism="Prosthecobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5333_length_5319_cov_18.784954"
                     /isolate="AMR_MDS_5629"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1965333"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1143
                     /locus_tag="PLP58_20335"
     CDS             <1..1143
                     /locus_tag="PLP58_20335"
                     /inference="COORDINATES: protein motif:HMM:NF024915.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HRJ10942.1"
                     /translation="QEAQRAGRGGRAQSIYASLIRQYPFTSSAAEAAYSSAVITRSES
                     DITTAFDAFQKFIDDHRASARFNDAVQQQFELAEEGRSGRRHRALLIIPVKMGGEDVV
                     KLYQQIIKNAPFGKLAPVAQFNIGEIYQDLGEKDKAVLAYQTVVDNYPNTKQAREAQF
                     RIGSISSVAASRSEDKSNLIATRDALTSYMTANPSGERSGEAQMILQTVNAAEAAQSL
                     EIAKFYERMGKTKAAAIYYNEALKYGAPEASKEARERLAELAAKDPEAVSAAQKGQPD
                     QDYTVPGARELKKRDDYIGPLAPELRRLGERPKMRAGDDNFLPIPLQEPALPVIPGVT
                     PPVGGTLLPAMEQTRPAVLPVEPPALPVPPKPEALPVPPKPAAPPP"
     gene            1175..1687
                     /gene="lptE"
                     /locus_tag="PLP58_20340"
     CDS             1175..1687
                     /gene="lptE"
                     /locus_tag="PLP58_20340"
                     /inference="COORDINATES: protein motif:HMM:NF016290.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0043165 - Gram-negative-bacterium-type cell
                     outer membrane assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS assembly lipoprotein LptE"
                     /protein_id="HRJ10943.1"
                     /translation="MNFRLLACALGLGFLLSGCAGYQLGGQKPGHLAGVTKLAVPAFE
                     NLSLEPRLGALVTNAVIKQIQLTGAYEIVNEADAEAVLTGRIVRIRRSQFRSDRDNVL
                     RTSQLLLTLSTDYTIRDSGGGSILHRGAAAADSYTVLDRNVQNSEAQALEDAAQRLAA
                     NIATDLSEGW"
     gene            1687..2502
                     /gene="rsmI"
                     /locus_tag="PLP58_20345"
     CDS             1687..2502
                     /gene="rsmI"
                     /locus_tag="PLP58_20345"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009962138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0000451 - rRNA 2'-O-methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HRJ10944.1"
                     /translation="MESVDTPQTEPLDDAAELSSGTLPPALYLVATPIGNLEDITLRA
                     LHVLKNAGVIACEDTRHTGRLLARHGIHARLLSLNEHNEAARIPGLIEHLQSGGSAAL
                     VSDAGMPAVSDPGQRLIHAVIATGLRVEPVPGPSAVLAALAASGLPVTPFYFGGFLPH
                     KKGQRASELAAAAGRDCTSVYFESPFRLVDTLALLAETAPEHLVVVARELTKKFEEFR
                     RGPARNVLSHFQNKPPKGEITLVIAPAEIPKWMQRHEARAANFETAAPSRLRE"
     gene            2555..5014
                     /locus_tag="PLP58_20350"
     CDS             2555..5014
                     /locus_tag="PLP58_20350"
                     /EC_number="2.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009962139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen/starch/alpha-glucan phosphorylase"
                     /protein_id="HRJ10945.1"
                     /translation="MTPAPCELGNSPENFKESILNHLRFTLGAHVSTASRNDWWVATC
                     MAVRDRVMEHATKSRTLHRQQGVRRVHYLSLEYLMGRLLENNLRNSGLYDAASQALSE
                     MGKDLGEILESEPDMGLGNGGLGRLAACFMDSLSTLNLPAIGYGIHYEFGLFRQEFVD
                     GRQVEHPDAWMRDGSPWKIARPSYRQTVRLYGHVEQKFDDHGHPHFEWAGGKCLLGLP
                     WDIPIVGYDSATVNVLRLWQAQADEDFNFQIFNEGSYIEALREKVVAETVSKVLYPND
                     STESGKELRLVQQYFFVACSLGDIIRRFKNGYSLPWSEFPAKAAIQLNDTHPAVAIPE
                     LMRILVDEEGLPWNQAWKICQETFSYTNHTLLPEALEMWSVSLFERVLPRHLQIIYRI
                     NQQFLQNEVEACWPGDVEKKRVLSLIEERGGKKVRMAHLSVLGSHATNGVAALHTRLL
                     CERLFPEFHELYPQRFLNLTNGVTFRRWLDVCNPELSALITGTIGEAWKKDASLLRQL
                     DSSADDAGFRARYQEIKRGHKVVLAKIIQDSCGTHVSPDALFDVQIKRLHEYKRQHLN
                     LLNIVTLYREILDNPGADFHPRVFIFGAKAAPGYFLAKNIIHAINSVAAKVNNDPRVG
                     DKLKVVFIPNYGVSVAEKIIPAADLSEQISTAGKEASGTGNMKLALNGALTIGTLDGA
                     NVEIAEEVGDDNIFIFGLTVEEVTALRAKGYNPWDYYWASERLRDTIDWISSDFFTGD
                     ANDFKPLRHSLLDHGDPYLCLADFQSYVEAQARVDAAYKDRDRWIRMAVLNTARMGKF
                     SSDRTILEYADKVWKLPGVAV"
BASE COUNT         1025 a         1976 c         1520 g          798 t
ORIGIN      
        1 caggaggcgc agcgcgcggg ccgcgggggc agggcgcaga gcatctacgc ctcgctcatc
       61 cgccagtatc ccttcaccag ctccgccgcc gaggccgcct actccagcgc ggtgatcacg
      121 cgctcggaga gcgacatcac cacagccttc gacgccttcc agaagttcat agacgaccac
      181 cgcgccagcg cccgctttaa tgacgcggtg cagcagcagt tcgagctggc ggaggagggg
      241 cgcagcggcc gccgccaccg cgctttgctc atcatcccgg tgaagatggg cggggaggat
      301 gtggtgaaac tctaccagca gatcatcaag aacgcgccgt tcgggaagct ggcgccggtg
      361 gcgcagttca acatcgggga gatttaccag gacctcgggg agaaggacaa ggcggtgctc
      421 gcctaccaga ccgtggtgga caattacccg aacaccaagc aggcgcgcga ggcgcagttc
      481 cgcatcggct ccatcagcag cgtggccgcc agccgcagcg aggacaagtc caacctcatc
      541 gccacccgcg acgccctcac cagctacatg acggcgaacc cgagcggcga gcgctccggc
      601 gaggcgcaga tgatcctgca aaccgtcaat gcggccgagg cggcgcagtc attggagatc
      661 gccaaattct acgagaggat gggcaagacc aaggccgccg ccatttacta caacgaggcc
      721 ctgaaatacg gggcgccgga ggcctccaag gaagcgcgcg agcgcctggc cgaactcgcc
      781 gccaaggacc ccgaagccgt ctccgccgcc cagaaaggcc agccggacca ggattacacc
      841 gtgcccggag ccagggaact gaagaagcgc gacgactaca tcggccccct ggcccccgaa
      901 ctccgccgcc tcggcgagcg cccgaagatg cgcgccgggg atgacaactt cctgcccatc
      961 cccctccagg agccggccct gcccgtcatc cccggagtca ccccgccggt gggcggcacc
     1021 ctgctgccgg ccatggagca gacacgcccc gccgtgctgc cagtggagcc ccctgccctg
     1081 cctgtgccgc ccaagcccga ggcactcccc gtgccgccca agcccgccgc gccgccgccc
     1141 tgatccgcca ccgccatcct gacattgacc caagatgaat ttccgacttc tcgcctgcgc
     1201 cctcggtctc ggcttcctgc tcagcggctg cgcgggctac cagctcggcg gacagaaacc
     1261 cggccacctg gcgggtgtga ccaaactggc cgtccccgcc tttgaaaacc tctccctcga
     1321 gccgcgcctc ggcgccctgg tgaccaacgc cgtcatcaag cagatccagc tcaccggagc
     1381 gtatgagatc gtcaacgagg ccgacgccga ggccgtgctc accggcagga tcgtgcgcat
     1441 ccgccgctcg cagttccgct ccgaccgtga caacgtcctg cgcacctccc agctcctact
     1501 caccctgagc accgactaca ccatccgcga cagcggcggc ggcagcatcc tgcaccgcgg
     1561 cgccgccgcc gccgactcct acaccgtgct cgacaggaac gtgcagaact ccgaggcgca
     1621 ggcccttgag gacgccgccc agaggctcgc cgccaacatc gccaccgacc tcagcgaagg
     1681 ctggtgatgg aatccgtgga cacgccgcag acggagccgc tggatgatgc cgcggagctt
     1741 tcatccggca ccctgccccc ggcgctgtac ctggtggcca cgcccatagg caacctggag
     1801 gacatcaccc tgcgcgccct gcatgtgctg aaaaacgccg gggtcatcgc ctgcgaggac
     1861 acccgccaca ccggacgcct gctcgcccgc cacggcatcc acgcccgcct gctcagcctc
     1921 aacgagcaca acgaggcggc gcgtattccc ggattgatcg aacacctgca aagcggcggc
     1981 agcgccgccc tcgtctccga cgccggcatg cccgccgtct ccgacccagg ccagcgcctc
     2041 atccacgccg tcatcgccac cgggctgcgc gtggagccgg tgcctggacc gagcgccgtg
     2101 ctggccgcgc tggccgcctc cgggctgccg gtcacaccgt tttacttcgg cggctttctc
     2161 cctcacaaaa agggccagcg cgccagtgaa ctggccgccg cagccgggcg ggactgcaca
     2221 agtgtctatt ttgaaagccc cttccgtctc gtggacacgc tggcgttgct ggcggagacc
     2281 gcgccggagc atctcgttgt cgtcgcccgt gagttgacga agaagtttga ggaattccgg
     2341 cgcggcccgg cccggaatgt gctttcccat ttccaaaaca aaccgcccaa aggtgagatc
     2401 acactcgtca tcgcccccgc tgaaattccc aagtggatgc aaaggcacga agcccgtgcc
     2461 gcgaatttcg aaactgccgc cccttcccgt ctgagagagt gagagcagac acttgcccct
     2521 tgaaacttcg aatttcgatc ctcccccccc tcccatgaca cccgccccct gcgaactcgg
     2581 aaactccccg gagaacttca aagaatccat cctcaaccac ctgcgcttca ccctcggcgc
     2641 gcatgtctcc accgccagcc gcaatgactg gtgggtggcc acctgcatgg ccgtgcgcga
     2701 ccgcgtcatg gagcacgcca ccaagtcgcg caccctgcac cgccagcagg gggtgcgccg
     2761 cgtgcattac ctctcattgg aatatctcat gggcaggctc ttggagaaca acctgcgcaa
     2821 ttccggcctc tacgatgccg ccagccaggc gctttccgag atgggcaagg acctgggcga
     2881 gatcctcgaa agcgagcctg acatgggcct gggcaacggc ggcctcggcc ggctcgcggc
     2941 ctgcttcatg gacagcctca gcacgctgaa cctgcccgcc atcggctacg gcatccatta
     3001 tgagttcggg ctcttcaggc aggagttcgt ggacgggcgc caggtcgagc atcccgacgc
     3061 ctggatgcgc gacggcagcc cgtggaaaat cgcccgcccc agctaccgcc agaccgtgcg
     3121 cttgtatggc catgtcgagc agaaattcga cgaccacggc catccccact tcgaatgggc
     3181 gggcggcaag tgcctgctcg gcctgccgtg ggacatcccc atcgtcggtt atgacagcgc
     3241 cacggtgaac gtgctgcgcc tctggcaggc gcaggcggac gaggacttca acttccaaat
     3301 cttcaacgag ggcagctaca tcgaggcgct gcgggagaaa gtcgtcgccg agaccgtctc
     3361 caaagtcctt tatcccaacg actccacgga aagcggcaag gagctccgcc tcgtgcagca
     3421 gtacttcttt gtcgcctgct cgctgggcga catcatccgc aggttcaaaa acggctacag
     3481 cctgccctgg agcgagttcc ccgccaaggc ggccatccag ctcaacgaca cccatcccgc
     3541 ggtcgccatc ccagagctca tgcgcatcct cgtggacgag gaggggctgc cctggaacca
     3601 ggcgtggaaa atctgccagg aaaccttttc ctacaccaac cacaccctgc ttcccgaagc
     3661 cctggaaatg tggagcgtct cgctcttcga gcgcgtcctc ccccgccatc tccagatcat
     3721 ctaccgcatc aaccagcagt tcctccaaaa cgaggtcgag gcgtgctggc ccggagacgt
     3781 ggagaaaaag cgcgtccttt ccctcatcga ggagcgcggc ggcaaaaaag tgcgcatggc
     3841 ccacctttcc gtgctcggca gccacgccac caacggagtc gccgccctgc acacgcggct
     3901 gctttgcgag cggcttttcc ccgagttcca cgagctttac ccgcagcgct tcctcaacct
     3961 caccaacggc gtcaccttcc gccgctggct ggatgtgtgc aatccggagc tctccgccct
     4021 catcaccggc accatcggcg aggcgtggaa aaaggacgcc tcccttctcc gccagctcga
     4081 ctccagcgcg gatgacgccg gcttccgcgc gcgctaccag gagatcaagc gcgggcacaa
     4141 ggtcgtgctc gccaaaatca tccaggactc ctgcggcacg cacgtcagcc ccgacgcgct
     4201 cttcgacgtg cagatcaagc gcctgcatga atacaagcgc cagcatctca acctgctcaa
     4261 catcgtcacc ctctaccgcg agatcctcga caaccccggc gcggacttcc acccgcgcgt
     4321 cttcatcttc ggcgccaagg ccgcccccgg ctacttcctg gccaaaaaca tcatccacgc
     4381 catcaactcc gtcgccgcca aagtgaacaa cgacccgcgc gtgggtgaca agctcaaagt
     4441 cgtcttcatc ccgaactacg gcgtctccgt cgccgaaaag atcattcccg ccgccgacct
     4501 ctccgagcag atttccaccg ccggcaagga agcctccggc accggaaaca tgaagctcgc
     4561 cctcaacggc gccctcacca tcggcaccct tgacggcgcc aatgtcgaga tcgccgagga
     4621 ggtcggggac gacaatatct tcattttcgg actcaccgtc gaggaggtca ccgccttgcg
     4681 cgccaagggt tacaacccct gggactacta ctgggccagc gagcgcctgc gcgacaccat
     4741 tgactggatc agcagtgact tcttcaccgg cgacgccaac gacttcaagc ccctccgcca
     4801 cagcctgctc gaccacggag acccctacct ctgcctggcc gactttcaaa gctacgtcga
     4861 agcccaggcc agggtggacg ccgcctacaa agaccgcgac cgctggatca ggatggccgt
     4921 gctcaacacc gcccgcatgg gcaagttcag cagcgaccgc accatccttg agtacgccga
     4981 caaggtctgg aaactgcccg gggtggctgt ttgagaggca acgcccaggc gcgacatggc
     5041 ctgaagtttg ggccggcggc gcccggacac cgtgcccagg aacgcgccca cacctccctt
     5101 ccgcctccag ccggcggtgg acggagcgtg agtattcccg ccgccatccc aagcccaggc
     5161 tccagcccgc aaccactctg gttgcgggcc gggggagact cccggaggcg gcaaccacta
     5221 tggttactag aatgggcagc cggttgcagg ctgcgaaaag cgcgattttg cgcggagcgc
     5281 atctccaaga cccccgccag caccccccac acccgcccg
//
LOCUS       DAOMYJ010000298         5302 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Prosthecobacter sp. isolate AMR_MDS_5629
            NODE_5363_length_5302_cov_20.735277, whole genome shotgun sequence.
ACCESSION   DAOMYJ010000298 DAOMYJ010000000
VERSION     DAOMYJ010000298.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867850
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prosthecobacter sp. (wastewater metagenome)
  ORGANISM  Prosthecobacter sp.
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Verrucomicrobiaceae; Prosthecobacter.
REFERENCE   1  (bases 1 to 5302)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5302)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.30
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:34:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,742
            CDSs (total)                      :: 4,684
            Genes (coding)                    :: 4,676
            CDSs (with protein)               :: 4,676
            Genes (RNA)                       :: 58
            tRNAs                             :: 55
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5302
                     /organism="Prosthecobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5363_length_5302_cov_20.735277"
                     /isolate="AMR_MDS_5629"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1965333"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..319
                     /locus_tag="PLP58_20355"
     CDS             <1..319
                     /locus_tag="PLP58_20355"
                     /inference="COORDINATES: protein motif:HMM:NF018533.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="zinc finger domain-containing protein"
                     /protein_id="HRJ10946.1"
                     /translation="TGVGNIYACEALFMAGIRPDKAAGKVSRVKLARLVEAIREVLAA
                     SIEMGGTTLRDFVNEKGEPGYFQQTLRVYDREGGPCRVCGARIRRMVMSNRSTFFCPR
                     CQK"
     gene            complement(323..703)
                     /locus_tag="PLP58_20360"
     CDS             complement(323..703)
                     /locus_tag="PLP58_20360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009959493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019239 - deaminase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rid family detoxifying hydrolase"
                     /protein_id="HRJ10947.1"
                     /translation="MTLVNDTANVPAAVGPYSQAARSGGFLFCSGQIPINPASGKIEA
                     ADVEGQARQVLANIESLLTAHGRAMRDVVKATVFLQSMADFPKVNPIYEAAFKGHKPA
                     RSTVQVAGLPLGALIEIEVVVEES"
     gene            complement(728..1942)
                     /gene="glgA"
                     /locus_tag="PLP58_20365"
     CDS             complement(728..1942)
                     /gene="glgA"
                     /locus_tag="PLP58_20365"
                     /EC_number="2.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009961524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004373 - glycogen (starch) synthase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen synthase"
                     /protein_id="HRJ10948.1"
                     /translation="MKSLFLTNEYPPHIYGGAGVHVEYLTRELAKLMEVEVRCFGGQE
                     SSAPNLAVHGAPLDASSWTCPANLKSVFGALQRCLDFNTRAIDADIVHLHTWYSHFGG
                     ILAKLNYGTPMVLTVHSLEPLRPWKREQLGGGYDFTVWLERTALEMADAVIAVSEETK
                     YDLLRLFNLDPHKIHVIHNGIDLDEYRKVEAPDVLLKHGVDPAVPFVLFVGRITRQKG
                     IIHLVRAIDRMDPGFQVVLCAGAPDTPEIAAEMQAAVAAAQAKRGGIHWISAMLPVEE
                     KIAMYSHADVFCCPSIYEPFGIINLEAMACETAVVASAVGGIKEVVVHGETGLLVPLD
                     QQNESPFEALEPELFAGDLARAVNQLMADPAQRRSMAGAGRRRAEERFSWTSIAHRTR
                     ALYESLMRQPNA"
     gene            complement(2161..3840)
                     /gene="ggt"
                     /locus_tag="PLP58_20370"
     CDS             complement(2161..3840)
                     /gene="ggt"
                     /locus_tag="PLP58_20370"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009966081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HRJ10949.1"
                     /translation="MKPLPLVCAAAVLSAVSAACGAEEAGYEKFAVATVHPLATDAAL
                     AAYEMGGNAVDAAVAAGLTLGVVDGHNSGIGGGCFFVIRAADGTVTAIDGREMAPAAA
                     HRDMYVIDGRLDSEASKTGALAPGVPGYLKACDLALRKHGKLTLADLLSPAADLAEKG
                     FPIDEVYERKLAAVAEKMRAFPASAGIFLKPDGVPLKKGDILVQKDLAATYRAIARNG
                     LEWFYGGDFAERAEAWMKANGGIMTRADFAAYRAVERQPVRASYRGYDLIAMPPPSSG
                     GVHVAQILGMLERHPLKHLRTASRVHLVTEAMKLAFADRAHWLGDPDFAKVPRGLVDA
                     DYLAELAGRIDLDRATPVPAHGTPPRWEGDIFGKHTTHLSAADAEGNWVALNQTINTG
                     FGSKVVVPGTGVVLNNEMDDFSVQPGVPNAFKLVGAEANAIAPGKRPLSSMSPTLVLK
                     DGKPVLSVGAAGGPTIITQALLLITGVLDDGLGPNAALAQPRFHHQWAPDELKIETGF
                     GESVLAEVEDFGHKLSLSTRFGACQCVMLDEKRGLLVPAHDPRVPGKSAGR"
     gene            3951..>5302
                     /gene="leuC"
                     /locus_tag="PLP58_20375"
     CDS             3951..>5302
                     /gene="leuC"
                     /locus_tag="PLP58_20375"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009958268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase large subunit"
                     /protein_id="HRJ10950.1"
                     /translation="MKFEFPSPTPVLRWVPSITHVTHTMGQTLFEKVWESHAVGTLAN
                     GQTQLLIDTHLVHEVTSPQAFGMLRDLGLKVAYPHRTFATVDHIVPTDNQQSPFADPL
                     AEAMIQELNKNAREFGITYFSLSSGRQGIVHVVGPEQGITQPGTTIACGDSHTATHGA
                     FGAIAFGIGTTQVRDILATQTMALSKMKVRRINVDGRLRPGVYAKDVILHIIRLLGAN
                     GGIGYAYEYGGSLFDSFSMEERMTVCNMSIEGGARCGYVNPDDKTFEYLKGRPYSPAG
                     DAWDDAVAKWRATASDPDARYDDIVSIRAEDVPPTVTWGTSPDQAIAVTENVPTPESA
                     ATGPARVSIQEALDYMKLPAGQPIKGTKVDVAFIGSCTNGRLSDFREVAKHIQGRKVA
                     RGVKAIAVPGSQIVAAMAEKEGLDKIFTAAGFEWRGAGCSMCLAMNPDKLVGDQLCAS
                     S"
BASE COUNT          971 a         1816 c         1668 g          847 t
ORIGIN      
        1 cacgggcgtg ggcaacatct acgcctgcga ggcgcttttc atggccggca tccggcctga
       61 caaggcggcg ggaaaggtca gccgcgtgaa gctggcgcgc ctcgtggagg ccattcgcga
      121 ggtgctcgcc gcctccatcg agatgggcgg caccacgctg cgcgattttg tgaatgagaa
      181 gggcgagccg ggttatttcc agcaaacgct gcgcgtctat gaccgcgagg gcgggccctg
      241 ccgtgtgtgc ggcgcgcgca tccgccgcat ggtgatgagc aaccgctcca ctttcttctg
      301 cccgcgctgc cagaaataag gatcaggact cctcgacgac gacctcgatc tcgatgagcg
      361 cgccgagagg cagcccggcc acttgcacag tggagcgcgc tggcttgtgg cctttgaagg
      421 cggcctcata gatcggattc accttgggga aatctgccat gctttgcagg aacaccgtgg
      481 ccttgaccac atcgcgcatg gcccggccat gcgccgtgag cagcgattcg atattcgcca
      541 gcacctgccg cgcctgccct tcgacatccg cagcctcgat tttaccgctg gcggggttga
      601 tggggatctg cccggagcag aaaagaaagc cgccggagcg cgccgcttgc gaataggggc
      661 cgactgcggc gggcacattg gctgtgtcgt tgacgagggt catggctgga gatgatggcg
      721 tttggattca agcgttcggc tggcgcatca gggattcgta aagcgccctg gtccggtggg
      781 cgatgctggt ccaactgaag cgctcctcag cacggcggcg tccggctccg gccatgctcc
      841 ggcgttgggc aggatcggcc atgagctggt tcaccgcccg cgccaggtcg ccggcaaaaa
      901 gctccggctc cagcgcctcg aaagggcttt cattctgctg gtcgagcggc acgagcaggc
      961 cggtttcccc atgcacgacg acctccttga tgccgcccac ggcgctggcg acgacggcgg
     1021 tctcgcaggc catcgcctcc aggttgataa tgccgaacgg ctcgtagatg gacgggcagc
     1081 agaacacatc agcgtgcgag tacatggcga tcttttcctc caccggcagc atggcgctga
     1141 tccagtggat gccgccgcgt tttgcctggg cggcggcgac cgcggcctgc atctcggcgg
     1201 cgatctcggg cgtgtccggc gcgccggcgc agagcacgac ctggaaaccc ggatccatcc
     1261 ggtcaatggc gcgcacgagg tggatgatgc ccttctggcg cgtgatgcgg ccgacgaaga
     1321 ggacgaaggg cacggcgggg tccacgccgt gtttgagcag cacatcgggc gcctccacct
     1381 tgcggtattc atcaaggtca atgccgttgt ggatgacgtg gattttgtgc gggtcgagat
     1441 tgaagaggcg cagcaggtcg tacttcgtct cctcggaaac agcgatgacc gcgtcggcca
     1501 tctccagcgc ggtgcgctcc agccagacag tgaagtcgta gccgcctcca agctgctcgc
     1561 gcttccaggg gcggaggggc tccagcgagt ggacggtgag caccatgggc gtgccatagt
     1621 tcagcttcgc caggatgccg ccgaagtggg aataccaggt gtgcagatgc acgatgtcgg
     1681 cgtcaatggc gcgcgtgttg aagtccaggc agcgctgcag cgcgccgaag acggatttca
     1741 aattcgcggg gcaggtccat gacgaggcgt cgagaggcgc gccatgcacg gccaggttcg
     1801 gagcggagct ttcctgcccg ccgaagcagc gcacctccac ctccatcagc ttcgccagct
     1861 cgcgcgtcag atattccaca tgcacgcccg cgccgccgta aatgtgcgga gggtactcgt
     1921 tggtgagaaa aagggatttc atggacagcg cccatctagc gcctgccagc gcgcatctgc
     1981 aagcctgtga tgcgccctca tcgcagatgg cgcggggcgt tgaatggggg cagtgacttc
     2041 accgcggagt gcgcatatca ataatcaacg ctcacaggcg tcaatgccag atgcggcgat
     2101 aaactgacag gaatggcggc atcaatgaga ccctgccgat tcagtgccgg agatgcaaac
     2161 tcacctgccg gcggacttgc ccggcacacg cggatcgtgc gcgggcacga gcaggccgcg
     2221 cttttcatcg agcatcacgc actggcaggc gccgaagcgg gtggagaggc tgagcttgtg
     2281 gccgaaatcc tccacctcgg cgagaacgga ctcgccaaag ccggtctcga tcttcaactc
     2341 atcgggcgcc cattgatgat ggaagcgcgg ctgcgccagg gcagcgttcg gacccaggcc
     2401 gtcatccagc acgccggtaa tgagcagcag ggcctgggtg atgatggtgg ggccgccggc
     2461 ggcgccgacg ctgagaacgg gtttgccgtc cttgagcacg agcgtggggc tcatgctgga
     2521 gagcgggcgc ttgccgggcg cgatggcgtt ggcctcggcg ccgacgagct tgaaggcgtt
     2581 cggcacgccg ggctgcacgg agaagtcgtc catctcgttg ttgagcacca caccggtgcc
     2641 gggcaccaca accttgctgc cgaagccggt gttgatggtc tggttgagag ccacccagtt
     2701 gccctcggcg tcggcggcgg agaggtgcgt ggtatgtttg ccaaagatgt cgccctccca
     2761 gcgcggaggc gtgccgtgcg cgggcacggg cgtggcgcgg tccaggtcaa tcctccccgc
     2821 cagctccgcc aggtagtccg cgtccaccag gccgcgcggc actttggcaa agtcggggtc
     2881 gcccagccag tgagcgcggt cggcgaaggc gagcttcatc gcctcggtca ccagatgcac
     2941 gcggctggcc gtgcgcaggt gcttgagcgg atggcgctcc agcatcccca gaatctgcgc
     3001 cacatgcacc ccgccgctgc tcggcggcgg catggcgatc aagtcgtacc cccggtagct
     3061 ggcgcgcacc ggctgccgct ccacggcgcg gtaggcggcg aagtccgccc gcgtcatgat
     3121 gccgccattc gccttcatcc acgcctccgc gcgctcggcg aaatcaccac cataaaacca
     3181 ttccagcccg ttccgtgcga tggcgcggta ggtggcggcc agatccttct gcaccaggat
     3241 gtcccctttc tttaatggca ctccatccgg cttcaaaaaa atgcccgccg acgccgggaa
     3301 ggcccgcatt ttctcggcca cagccgcgag cttgcgctca taaacctcgt caatggggaa
     3361 gcctttctcg gcgagatcgg cggcaggtga aagcaagtca gccagagtca gtttgccatg
     3421 cttgcgcagc gccaagtcgc acgccttcag gtagccgggg acaccgggcg cgagcgcgcc
     3481 ggtcttgctg gcctcgctgt cgagcctgcc gtcaatgaca tacatgtccc ggtgagcggc
     3541 ggcgggcgcc atttcgcgcc cgtcaatggc ggtgacagtc ccatcagcgg cgcggatgac
     3601 aaagaagcag ccgccgccga tgccggagtt gtggccatcc acgacaccga gggtcagtcc
     3661 ggcggcgacg gcagcatcca cggcgttgcc tcccatttca taggcggcca gcgccgcatc
     3721 ggtggccagc ggatgcaccg tggcgacggc gaacttttca taccccgcct cctccgcgcc
     3781 gcaggcggcg gagacagcgg acaaaacggc ggcggcgcag acgaggggca ggggcttcat
     3841 gagggaaaat ggcacgcccc acgcatagcc gccaccgcca tgaaagccag cgcgcctgcc
     3901 tccccaaaac gcgcggtgcg cgcgccccgc cggcgcgcca cccccaaaac atgaaattcg
     3961 aatttccttc ccccaccccc gtgctacgct gggtgccctc aatcacgcac gtcacacaca
     4021 ccatgggcca aacactcttc gaaaaagtct gggaatctca cgccgtcggc accctcgcca
     4081 acggccagac gcagctcctc attgacaccc acctcgtcca cgaagtcacc agcccccagg
     4141 ccttcggcat gctccgcgat ctcggactca aagtcgccta cccccaccgc accttcgcca
     4201 ccgtggacca catcgtgccc accgacaacc agcagtcccc cttcgccgac cccctcgccg
     4261 aagccatgat ccaggagctg aacaaaaacg ccagggagtt cggcatcacc tacttcagcc
     4321 tcagcagcgg caggcagggc atcgtccacg tcgtcggccc cgagcagggc atcacccagc
     4381 ccggcaccac catcgcctgc ggagacagcc acactgccac gcacggcgcc ttcggcgcca
     4441 tcgccttcgg catcggcacg acgcaagtgc gcgacatcct cgccacccag accatggcct
     4501 tgagcaaaat gaaggtccgc cgcatcaacg tggacggccg cctgcgcccc ggcgtctatg
     4561 ccaaggacgt catcctccac atcatccgcc tcctcggcgc caacggcggc atcggctacg
     4621 cctacgaata cggcggcagc ctcttcgaca gcttctccat ggaggagcgc atgaccgtct
     4681 gcaacatgtc catcgaaggc ggcgcccgct gcggctacgt caaccccgat gacaagacct
     4741 tcgaatatct caaaggccgg ccctattccc ccgccggtga cgcctgggat gacgccgtcg
     4801 ccaaatggcg cgccaccgcc tccgaccccg acgcccgcta cgacgacatc gtcagcatcc
     4861 gcgccgagga cgtgcccccc accgtcacct ggggcaccag ccccgaccag gccatcgccg
     4921 tcaccgagaa tgtgccgacg cccgaaagcg cggccaccgg accagcccga gtctccattc
     4981 aggaagctct cgactacatg aagctgccag ccggccagcc catcaaaggc accaaagtgg
     5041 acgtggcttt catcggctcc tgcaccaacg gccgcctgag cgacttccgc gaagtcgcca
     5101 aacacatcca gggccgcaaa gtcgcccgcg gcgtcaaagc catcgccgtc cccggctccc
     5161 agatcgtcgc cgccatggcc gagaaggaag gcctcgacaa aatcttcacc gccgcaggct
     5221 tcgaatggcg cggcgccggc tgctccatgt gcctggccat gaaccccgac aagctcgtcg
     5281 gagaccagct ctgcgcctcc tc
//
LOCUS       DAOMYJ010000299         5241 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Prosthecobacter sp. isolate AMR_MDS_5629
            NODE_5452_length_5241_cov_23.117817, whole genome shotgun sequence.
ACCESSION   DAOMYJ010000299 DAOMYJ010000000
VERSION     DAOMYJ010000299.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867850
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prosthecobacter sp. (wastewater metagenome)
  ORGANISM  Prosthecobacter sp.
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Verrucomicrobiaceae; Prosthecobacter.
REFERENCE   1  (bases 1 to 5241)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5241)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.30
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:34:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,742
            CDSs (total)                      :: 4,684
            Genes (coding)                    :: 4,676
            CDSs (with protein)               :: 4,676
            Genes (RNA)                       :: 58
            tRNAs                             :: 55
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5241
                     /organism="Prosthecobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5452_length_5241_cov_23.117817"
                     /isolate="AMR_MDS_5629"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1965333"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..3066
                     /locus_tag="PLP58_20380"
     CDS             <1..3066
                     /locus_tag="PLP58_20380"
                     /inference="COORDINATES: protein motif:HMM:NF012311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HRJ10951.1"
                     /translation="NGAGVSGVAPRATLVGFRLIADLFSAEDEAEAMARGMDLIQVKN
                     NSWGILDGYPWELGQTGALFDAAMSQAATLGRGGLGTISVWSAGNGRHQGDQGGKDGY
                     ASNRYCITVGAVTNKGALAFYSETGAHLCVVAPSAENRAGIVTTDLAGIRGYNDGLNL
                     KNLGDIDYTNDFSGTSAAAPVVAGVAALMLEANPNLNWRDVKEILLRSSTQIGGSSGW
                     VSRDARAELGLPAVKHHHSFGGGLVNARAAVDMAKTWTSLGPEVEIVKSAVSTESGPA
                     SIKGGTTILIPEETKKTLKVNRMDLDFSASTALRVEHVAVTVSITNTKRGDMTVKLVS
                     PSGVVSVLAPASAQDVGSDYSDWTFTSLRHWGESSRGVWSIITHEPDDGSTGKINAAA
                     IRLFGTAWPAAEIVTAPQDALLATGDSLAFTGAVSGQAPLTRQWLKNGKVIAGAAGDS
                     FSIASVKLADAGAYDYIVSNLTGLDGARALLGVVDRVVASQNLVAGRTATFTARTAGP
                     STLRHQWFKGAQMLEDDGRVTGARSPVLTLRNVSNADAEDYFCRVSLGELSLDTLRGT
                     LGIIQKPVLEAPDPGSFFTFASGYVEYQVNADNAPDSFTARGLPPGLKLDPKTGLISG
                     RPDKPGIYTVTISASNAAGSSAPVTAVVEIAPLPQGTTGVFTGLLDRLFYYNGGHGGF
                     VTLTVNESGRFTGRIIRGGFYTTNFTGRLDIDPQAQAPEATVLIPRAAPHPPLELFFA
                     LVDGVLEGDLSAPGEEGIRVRGFRQVKSATPASVAGRYNMPLRTVQSDAAYPLGTGHL
                     SAVLSAQGAVNFSGKLADGTTLTGSGIPASGGELPLHRMLYNNTGSIQGLLILDRGTP
                     SFEAALDWGKNTQAPGSTRSYAAGFPVHDIGGGGGRYLPPATGQPVMGLPLLDNNAVL
                     VFTEGGLAAPFSQSLTLGAGNKVTLPSGAANPHQIRVTLNSANGLVTGTGKAMDIDPD
                     NPGLNRQRQGSMSALIIQGEARAEGYFLLPQDQGKTSPIWSGRVMLQAD"
     gene            complement(3160..4950)
                     /locus_tag="PLP58_20385"
     CDS             complement(3160..4950)
                     /locus_tag="PLP58_20385"
                     /inference="COORDINATES: protein motif:HMM:TIGR02603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HRJ10952.1"
                     /translation="MLKNALATALLQRAEDKDDPMIPLLIWYGIEPLVGADAQAGLEL
                     ARVSKMPKVTQFIYRRLGAEQAGRTALLRVAADSEDVEERESLLKSVVEAARAGNKIA
                     KPADWDTLRDRLMATQMTESPRTPEMGPPAKGILVAELEAFMGIGPAIAAFRERLTST
                     APLDARVAALKLLVQIRDAQTAPILHTLLGDGQEAHPTLRRAAIQALATLPHPRTPAM
                     LAGLLPALAPVVKNDAINTLATTPEGSKTLLLAVKEEKVAKSLLSPFLARQMDALKNA
                     EVSALLKEVWGDLNAPKPDLEQRKQKLRAMLTPDALAKADLAQGKTIYSAVCGTCHTL
                     FGQGAKVGPDLTGSNRANLDYLLDNVLDPNAVIGKDYQLNIFELADGRLASGIIKEES
                     PAAWKVAMPGGIEQLITKAEVKKRTVSPVSTMPEGLFDALPQEQLLALVAYLQSPGGD
                     AGAGAGMIEAEKLQAAVSRGKTAPQGMGNFRDGRWSGDSHLFWTGGKPGDTLTLSFPV
                     AEAGKRKVYAVLTKAPDYGSVRLTVNGAAASTVEMDLYDAKVTNTEEVLLGEFDFKPG
                     AQKLEITISGANPKARPGRWYVALDYLRVE"
BASE COUNT          919 a         1792 c         1625 g          905 t
ORIGIN      
        1 aacggcgcgg gcgtcagcgg cgtggcaccg cgcgcgaccc tggtgggctt ccgcctcatc
       61 gcggacctgt tcagcgccga ggatgaggcg gaggcgatgg cacgcggcat ggacctcatt
      121 caggtcaaaa acaacagttg gggcatcctg gacggatatc catgggagct gggtcagacc
      181 ggcgcgctct ttgacgccgc catgagccag gcggccactc tgggacgcgg agggctgggc
      241 accatctccg tctggtccgc gggcaacggc cgccaccagg gcgaccaggg cggcaaagat
      301 ggttatgcca gcaaccggta ctgcatcacc gtcggcgcgg tgacgaacaa gggcgcgctg
      361 gccttttaca gcgaaaccgg cgcgcatctc tgcgtggtcg cgccctcggc ggaaaaccgc
      421 gccggcatcg ttaccacgga cctcgccggc attcgcggct acaatgacgg gctgaacctg
      481 aaaaatctgg gcgacattga ctacaccaac gacttcagcg gcacctccgc cgccgcgccc
      541 gtcgttgccg gtgtcgccgc cctcatgctg gaggcaaatc cgaacctcaa ctggcgggat
      601 gtgaaagaaa tcctgctgcg ctcctccacc cagatcggcg gcagctccgg ctgggtttcg
      661 cgcgatgcca gagcggagct gggtttgcca gcggtgaagc atcatcactc gttcggcggc
      721 ggtctggtca acgcgcgcgc ggcagtggac atggcaaaaa catggacctc cctcggcccc
      781 gaggtggaaa ttgtgaaaag cgctgtgagc accgagtccg gccccgcctc gatcaagggc
      841 ggcaccacca tcctcatccc cgaggagaca aaaaagaccc tcaaagtcaa ccgcatggac
      901 ctcgacttca gcgcctccac cgccctgcgc gtcgagcatg tggcggtgac ggtttccatc
      961 acgaacacca aacgcggcga catgacggtg aaactcgtct ccccctccgg tgtcgtcagc
     1021 gtgctggctc cggcctccgc gcaggatgtg ggctcggatt acagtgactg gaccttcacc
     1081 agcctgcgcc actggggcga atcctcgcgc ggagtgtgga gcatcatcac tcacgagcct
     1141 gatgatggct ccaccggcaa gatcaatgcc gccgccatcc gtctattcgg caccgcctgg
     1201 cctgccgccg aaatcgtcac cgccccgcag gatgccctgc tcgccaccgg tgacagcctc
     1261 gccttcactg gcgcggtctc cggccaggcc ccgctcaccc gccagtggtt gaaaaacggc
     1321 aaggtcatcg caggcgcggc gggcgactcc ttcagcatcg cctccgtcaa gctcgcggac
     1381 gcgggagcct atgactacat cgtgtccaac ctcaccggcc tcgatggtgc gcgcgccttg
     1441 ctcggcgtgg tggaccgcgt ggtggcatcg caaaacctcg tcgccggcag gaccgccact
     1501 ttcaccgcgc gcacggcggg accatccacg ctccgccatc aatggttcaa aggcgcgcaa
     1561 atgctggagg acgacggccg cgtcaccggc gcgcgctctc ccgtgctcac gctgcgcaat
     1621 gtcagcaacg ccgacgcgga ggattacttc tgccgagtca gcctcggcga gctttccctc
     1681 gacaccctgc gcggcacttt gggcatcatc cagaagcccg tccttgaggc gcccgatccc
     1741 gggtcgttct tcacctttgc cagcggctat gtcgagtatc aggtcaatgc cgataacgcg
     1801 cccgacagct tcaccgcgcg cggcctgccg cctgggttga agctcgatcc gaagaccggc
     1861 ctcatctccg ggcgccccga caagccgggc atctacacgg tgacgatctc tgccagcaac
     1921 gcggccggga gcagcgcgcc cgtgaccgcc gtggtggaga tcgccccgct gccgcaggga
     1981 accacaggag tcttcacagg cctgctggac cgtctctttt attacaacgg cggccatggc
     2041 ggcttcgtca ctctcacggt gaacgaaagc ggccgcttca caggccgcat catccgcggc
     2101 ggcttttaca ccaccaactt cacaggcagg ctcgacattg atccgcaagc ccaggccccc
     2161 gaagccaccg tcctcattcc ccgcgccgcg ccgcatccgc cactggagct gtttttcgcc
     2221 ctcgtggatg gagtcctgga gggcgacctc agcgcgcctg gcgaggaggg catccgcgtc
     2281 cggggcttcc gccaggtaaa atccgccact cccgcctccg tggccgggcg ctacaacatg
     2341 ccgctgcgca ccgtgcaaag cgacgccgcc tatccgctcg gcaccggtca cctctccgcc
     2401 gtgctttcgg cgcagggagc ggtgaatttt tccggcaaac tcgccgacgg caccaccctc
     2461 accggctccg gcatcccggc ctccgggggc gagctgccgc tgcaccgcat gctctacaac
     2521 aacaccggct ccatccaggg ccttctcatc ctcgaccgcg gcacgccctc gtttgaggcg
     2581 gcgctcgact ggggcaaaaa cacgcaggct cccggctcca cgcgcagtta cgcggcggga
     2641 ttccccgtgc atgacattgg cggcggcggc gggcgctacc tgccgcccgc gaccggccag
     2701 cctgtgatgg gcctgccctt gctcgacaac aacgccgtgc tcgtcttcac cgaaggcggc
     2761 ctcgccgcgc ccttcagtca gtcattgacc ctcggcgcgg gaaacaaagt cacactgccc
     2821 tccggcgcgg ccaacccgca tcaaatccgt gtcaccctca acagcgccaa cggcctcgtc
     2881 accggcacgg gcaaggccat ggacattgac ccggacaatc cgggcctcaa ccgccagcgc
     2941 cagggcagca tgtccgcgct catcatccag ggcgaggcgc gggcggaggg ttattttctc
     3001 ctgccccagg accagggcaa aacctcgccc atctggtcgg gccgggtgat gctccaagcg
     3061 gactgacgcg gccgtagcgc tcgtagagga agtcgtgaga cttccgggtt tgaaaggaag
     3121 ggtgacgcac ccgcgaaaca tccaaatccc cgaattccct cactccacac gcaggtaatc
     3181 cagagcgacg taccaacggc cgggccgggc tttcgggttg gcgccggaga tcgtgatctc
     3241 gagcttttgc gcgccgggtt tgaagtcgaa ctcgccgagc agcacctctt cggtgttggt
     3301 gactttggca tcgtaaagat ccatctccac cgtggaggcc gccgcgccgt tcaccgtcag
     3361 gcgcacgctg ccgtagtcgg gggccttggt gagcacggca tagactttgc gcttcccagc
     3421 ctcagccacg gggaagctga gcgtgagcgt gtcgcccggt ttgccgccgg tccagaagag
     3481 atgggagtcg ccgctccagc gcccgtcgcg gaagttgccc atgccttgcg gcgcggtttt
     3541 gcctctggaa acggcggcct gcagcttctc cgcctcgatc atccccgcgc cagcgccggc
     3601 atcgccgccg gggctttgca ggtaggcgac cagggccagc agttgctcct ggggcaaggc
     3661 gtcgaacaag ccctcgggca tggtgctcac cggtgacacc gtgcgttttt tcacctcggc
     3721 cttggtgatg agctgctcga tgccgccggg catcgccacc ttccacgcgg caggggattc
     3781 ctccttgatg atgccgctgg ccaggcggcc atctgcgagt tcgaagatgt taagctggta
     3841 gtccttgcca atgaccgcgt tgggatcgag cacgttgtcg agcaggtagt cgaggtttgc
     3901 gcgatttgaa ccggtgaggt ctgggcctac cttggcaccc tgaccgaaca gcgtgtggca
     3961 ggtgccgcag acggcgctgt aaatcgtctt gccctgggcc aggtcggcct tcgccagcgc
     4021 atcgggcgtg agcatggcgc ggagcttctg tttgcgctgc tccaggtccg gcttgggcgc
     4081 gttgagatcg ccccagactt ccttgagcag ggcggaaacc tcggcgtttt tcagagcatc
     4141 catctgccgc gccaggaagg gcgagagcag gctcttcgcc accttctcct ccttcaccgc
     4201 cagcagcagg gtcttcgatc cctcgggcgt ggtggccagg gtgttgatgg cgtcgttttt
     4261 caccaccggt gccagcgcgg gcagcaagcc tgccagcatg gcgggtgtgc gcggatgcgg
     4321 cagtgtggcc agcgcctgga tggccgcgcg gcgcagcgtg ggatgagcct cctgaccatc
     4381 acctaacaag gtgtgcaaaa tgggtgccgt ctgcgcatcg cggatctgca ccagcagctt
     4441 caaagccgcc acccgcgcat ccagcggcgc ggtcgaggtc aggcgttcgc gaaaagcggc
     4501 gatggccggc ccgatgccca tgaaggcctc caactcggcg actaggatgc ccttcgcagg
     4561 cggccccatc tccggtgtgc gcggagactc ggtcatctgc gtggccatga ggcggtcgcg
     4621 caaagtgtcc caatcggcgg gtttggcgat cttgttgccc gcccgcgcgg cctcgaccac
     4681 ggacttcaag agcgactcgc gttcctccac atcctccgag tccgccgcca cgcgcagaag
     4741 agccgtgcgt cccgcctgct cggcgccgag gcggcgatag atgaactgcg tcaccttcgg
     4801 catcttcgac acccgcgcga gttccagccc cgcttgcgcg tcagctccca ccagcggctc
     4861 gatgccatac cagatgagca acgggatcat cggatcgtcc ttgtcctcgg cacgctggag
     4921 gagggcggtg gcgagggcgt ttttcaacac gagtccctgt ggcaaagcgg aaagcatgga
     4981 agcgagttcg cgacgcacaa cagcggattt actttccttg gcgagtttca acaggggggc
     5041 ggaataccag gttgcataag gttccttgag tgaactcgga ctatcagagt cttgacgcag
     5101 ttgatacggg atcaaatcag ccaaataacg aatggtccac ttttgcactt cagggcttgc
     5161 tgaccgaagt gcatcccgca aatccccccc gcctccgata gaagataaag tcaactcaat
     5221 ccacaaggat ctgagtgaga c
//
LOCUS       DAOMYJ010000300         5215 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Prosthecobacter sp. isolate AMR_MDS_5629
            NODE_5479_length_5215_cov_22.276357, whole genome shotgun sequence.
ACCESSION   DAOMYJ010000300 DAOMYJ010000000
VERSION     DAOMYJ010000300.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867850
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prosthecobacter sp. (wastewater metagenome)
  ORGANISM  Prosthecobacter sp.
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Verrucomicrobiaceae; Prosthecobacter.
REFERENCE   1  (bases 1 to 5215)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5215)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.30
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:34:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,742
            CDSs (total)                      :: 4,684
            Genes (coding)                    :: 4,676
            CDSs (with protein)               :: 4,676
            Genes (RNA)                       :: 58
            tRNAs                             :: 55
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5215
                     /organism="Prosthecobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5479_length_5215_cov_22.276357"
                     /isolate="AMR_MDS_5629"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1965333"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..556
                     /locus_tag="PLP58_20390"
     CDS             <1..556
                     /locus_tag="PLP58_20390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ10953.1"
                     /translation="QAAQAVETAANALPPAFEPNPFQQMQSAFLAQALDQLDQTLHPM
                     QGNQPQAGGEQQQQQGGSQQQQQAQQSLANARQSQQQSMASQRNQGQTPGSQQPGRQM
                     AQNPPGQNEGDPSQASSEGGNQSMQLVEGEIGAMLQLSGGDWGRLPGKMAADLSEATR
                     TEAAPEYRAAIESYYKAIATKARK"
     gene            complement(587..>832)
                     /locus_tag="PLP58_20395"
     CDS             complement(587..>832)
                     /locus_tag="PLP58_20395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ10954.1"
                     /translation="AGGKCSVHLWCAPCYGSALRVARQPARGGQSAGLAPAAIPKPRI
                     LANAPTRPPGSSASSSNSGPMPGVKSKAVRVRQALGD"
     assembly_gap    833..932
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <933..1125
                     /locus_tag="PLP58_20400"
     CDS             <933..1125
                     /locus_tag="PLP58_20400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ10955.1"
                     /translation="AATHAKCTLHLPPAAATHAKCTLHLPPAAATHTKCTLHWRRAGV
                     AWLLFLPGGAATCPPATTA"
     gene            complement(1412..1747)
                     /locus_tag="PLP58_20405"
     CDS             complement(1412..1747)
                     /locus_tag="PLP58_20405"
                     /inference="COORDINATES: protein motif:HMM:NF024886.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HRJ10956.1"
                     /translation="MQSIPDTTATFTRFPQLKSLRARTLESCLSWVARIARHHGAACA
                     SLLTQEQVLAFLHHVQQTKGCGGSTLNQCVCALRTCYRDHLERAGCRMAHGKAAKKLP
                     GMNGTQLRR"
     gene            complement(1833..2900)
                     /locus_tag="PLP58_20410"
     CDS             complement(1833..2900)
                     /locus_tag="PLP58_20410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ10957.1"
                     /translation="MLAAPAPAPLPEEAPVKKDNSQRRGIIASIIIASLVVHLAGLAL
                     FGLWIVARQFAKVEAVFQVRETLKIPLQKTPEHRMSVAAHEAAAPKPVFQDKLVSTRP
                     APFSLPELPKLDVQQMLPLDPSELVSDQVTSLVGTAGLGSGLGAGLSGSGGAGKALAN
                     FTFMGIKAEGRRIVLLFDVSGSVVNKANATGMPLGKIKEETLKLISGLPSGAQFGLVQ
                     FVRNYKPFRAEMTPVTPANRDLAAQWMESEWNESGQMPRGGSGVRSPEPNGVVCVLDA
                     AFELKPDVIFLISDGGFWQTYPQERRIPHEDLEDKMKELQKGRAERIPVHFIGFQMHP
                     NDKGAWERIVRRTGGKLREIK"
     gene            complement(2912..3361)
                     /locus_tag="PLP58_20415"
     CDS             complement(2912..3361)
                     /locus_tag="PLP58_20415"
                     /inference="COORDINATES: protein motif:HMM:NF014525.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HRJ10958.1"
                     /translation="MIRRRRRHDTAVEMQMGPMIDMVFLLLVFFMVTAKPLKPEADIP
                     LGLPGTAEQDEPLELPDEQKIVIQSSGAILLNESPLADAGDRELDKLHRTLFRLGEAA
                     KASQSELLVTLAPEDEARHQRIVDVLNACARAGISGVTFDDPGGEEN"
     gene            complement(3358..3765)
                     /locus_tag="PLP58_20420"
     CDS             complement(3358..3765)
                     /locus_tag="PLP58_20420"
                     /inference="COORDINATES: protein motif:HMM:NF014525.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HRJ10959.1"
                     /translation="MRRGKARQDEVGFNITPMIDLTFLLLIFFMVTSKLSKEQKKMDI
                     SLPAAASATQPEDLSNRDIVNLDGKGVLHLADRAMSDEQMKEYLEARFRGNPPLKIYL
                     RADRDTPGKRIREFMKLASEAGAISVIFGTYNK"
     gene            complement(3972..4514)
                     /locus_tag="PLP58_20425"
     CDS             complement(3972..4514)
                     /locus_tag="PLP58_20425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ10960.1"
                     /translation="MDEPSPPPIPRDLHCEYEERPFKHCTRCGETLEDDAGGFQVSKA
                     YKNGECVMEYALCDHCRTAMMEEFSQESKKRLSTFQDESVDLERGLHLCSVCGQGRDG
                     EGTDDYVITGVCEHDRLLHSIMICGQCADGVQELLSAKTKDTWRRFVDENFPGPPGEG
                     EYPEIEETKTTPMPVFSIKV"
     gene            4637..>5215
                     /locus_tag="PLP58_20430"
     CDS             4637..>5215
                     /locus_tag="PLP58_20430"
                     /inference="COORDINATES: protein motif:HMM:NF014016.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CbiX/SirB N-terminal domain-containing protein"
                     /protein_id="HRJ10961.1"
                     /translation="MQSPAPTSLTPHSQAALILAGHGSTVNPDSSAPTHSHADRIRRM
                     GVFREVVCCFWKEEPGMREVFESVDSAEVYVVPNFISEGYFCQQVLPRELGLDGPVTR
                     REGKVVYYCDPVGVHPSMTRLLLRRADEVAPGVPRAETSLVVAGHGTSLNENSRKAIE
                     NQVRLIREGGHGFAEVVDAYMEEPPLVSDWAEL"
BASE COUNT          854 a         1597 c         1764 g          900 t
ORIGIN      
        1 ccaggccgcc caggccgtcg agaccgccgc caacgccctg ccgcccgcct tcgagccgaa
       61 ccccttccag caaatgcagt ccgccttcct cgcccaggcc ctggaccagc tcgatcaaac
      121 cctccacccc atgcagggga accagccgca ggccggcggc gaacaacagc agcagcaggg
      181 aggcagccag cagcagcagc aggcgcagca aagcctcgcc aacgcccggc aaagccagca
      241 gcagagcatg gccagccagc gcaaccaggg ccagacaccc ggctcccagc agcccggccg
      301 gcagatggcg caaaacccgc ccggccagaa cgagggcgac ccctcccagg ccagctccga
      361 aggcggcaac cagtccatgc aactcgtcga aggcgagatc ggagccatgc tccagctcag
      421 cggcggcgac tggggccggc tgcccggcaa aatggccgcc gacctcagcg aagccacccg
      481 caccgaagcc gcccccgaat accgcgccgc catcgaaagc tactacaaag ccatcgccac
      541 caaggcgcgc aagtaagctt gatttttgag cggggggcag cggccttcaa tcaccaagcg
      601 cctgacgcac acggacggct ttggatttca cgcccggcat cgggccactg ttcgagctgg
      661 aggcggagct gcccgggggg cgtgtaggcg cattcgccag aatgcggggc ttgggtatcg
      721 cggcgggagc caaacccgcg ctttgcccgc cacgggcagg ctgccgcgcc acccgcaggg
      781 cggagccgta gcagggtgcg caccacaaat gcacgctgca tttgccacct gcnnnnnnnn
      841 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      901 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnggcggcga cacacgccaa atgcacgctg
      961 catttgccac ctgcggcggc gacacacgcc aaatgcacgc tgcatttgcc acctgcggcg
     1021 gcgacacaca ccaaatgcac gctgcattgg cggcgggcgg gcgtggcctg gctgcttttt
     1081 ttaccgggtg gtgctgccac ctgcccacct gccaccaccg catgaaaatc atcacctggg
     1141 actccggggt aacttgggat aatctcaatg cccgatgggg tgattcagca tctttgcttg
     1201 acgcagatgc ctggcaggaa tggtactcag ttaagacaag gggcggtgcc gtaggccggg
     1261 tgtgtccggc gaagaatcgc gcccctgaac tcaacaagcc ctcaccgccg gaccgcccgg
     1321 cttttggcgc gtgaatccca tgccggaggt gcgtgcacag ccgggcgggc taaagccaca
     1381 cccggcctac gaatcgtcat tccgccacga ttcagcgcct taactgagtg ccattcatgc
     1441 ctggcagctt tttggcagct tttccgtgag ccattcgaca tcccgcgcgc tccaggtggt
     1501 cgcggtaaca cgtgcgcagg gcgcagacgc actggttgag cgtgctgccg ccgcagccct
     1561 tggtctgctg gacatggtgg agaaaggcga gcacctgctc ctgggtgagc agggaggcgc
     1621 aggccgcgcc gtgatgcctg gcgatgcgcg cgacccagga gaggcagctc tccagcgtgc
     1681 gggcgcgcag ggacttgagt tgaggaaaac gggtgaaggt ggcggtggta tcgggtatgc
     1741 tttgcatagg gccgccaagc accgcctgag caggtttgcc tttccgcttt gccaggtgcg
     1801 ggacgggccg gatgaagaag cgaaagggcg ggttacttga tttcccgcag tttgccgccg
     1861 gtgcggcgga cgatgcgctc ccaggcgcct ttgtcgttcg ggtgcatctg gaagccgatg
     1921 aagtgaacgg ggatgcgctc ggcccggcct ttttgcagct ctttcatttt gtcctcgagg
     1981 tcctcgtgcg ggatgcggcg ctcctggggg taggtctgcc agaagccgcc gtcggagatg
     2041 aggaagatga cgtcgggctt cagttcgaag gcggcgtcga gcacgcagac gacgccgttg
     2101 ggctcggggc tgcgcacgcc ggagccgccg cgcggcatct ggccgctctc gttccattcg
     2161 gactccatcc attgggcggc gaggtcgcgg ttggcggggg tgacgggggt catctccgcg
     2221 cggaagggtt tgtagttgcg cacgaactgg acgaggccga actgcgcgcc gctgggcagg
     2281 ccgctgatga gcttgagggt ctcctctttg attttgccga ggggcatgcc ggtggcattg
     2341 gccttgttga cgacgctgcc tgagacatcg aagagcagca cgatgcgccg cccctcggcc
     2401 ttgatgccca tgaaggtgaa gttggccagc gccttgcccg cgccgccgga gccggagagg
     2461 cccgcgccca ggccggagcc gagtccggcg gtgccgacga ggctggtgac ctggtcgctg
     2521 accagctcgc tggggtcgag cgggagcatc tgctgcacgt cgagcttcgg cagctcgggc
     2581 agggagaagg gggcgggtcg ggtgctgacg agcttgtcct ggaagacggg cttgggcgcg
     2641 gcggcctcgt gcgcggccac gctcatgcgg tgctcgggcg tcttttggag ggggattttc
     2701 aatgtctccc gcacctggaa gacggcctcc actttggcga actgccgggc cacgatccac
     2761 aggccgaaga gcgcgaggcc ggcgagatgc acgacgaggc tggcgatgat gatggaggcg
     2821 atgatgcccc ggcgctggga gttgtctttt ttgaccggcg cctcctcagg cagcggcgcg
     2881 ggggctgggg cggcgagcac gatccgggac attagttttc ctcccctccc ggatcgtcaa
     2941 aggtgacgcc gctgattccg gccctggcgc aggcgttgag cacgtccacg atgcgctggt
     3001 gcctggcctc gtcctctggg gcgagggtga cgagcagctc gctctgggag gctttggcgg
     3061 cctcgccgag gcggaagagg gtgcggtgga gcttgtccag ctcgcggtcg ccggcgtcgg
     3121 cgagggggct ttcgttgagc aggatggcgc cgctgctctg gatgacgatt ttttgctcgt
     3181 cgggcagctc gaggggctcg tcctgctcgg cggtgccggg caggccgagg gggatgtcgg
     3241 cctcgggctt gaggggcttg gcggtgacca tgaagaagac gaggagcagg aagaccatgt
     3301 caatcatggg tcccatctgc atttccacag cggtatcgtg gcggcggcgg cggcggatca
     3361 tttgttgtag gtgccaaaga tgacgctgat ggcgccggcc tcgctggcca gcttcatgaa
     3421 ctcgcggatg cgcttgccgg gggtgtcgcg gtcggcgcgg aggtagattt tgagcggcgg
     3481 gttgccgcgg aagcgggcct ccaggtattc tttcatctgc tcgtcgctca tggcgcggtc
     3541 ggcgaggtgg agcacgccct tgccgtcgag attgacgatg tcgcggttcg agaggtcctc
     3601 cggctgcgtg gcgctggcgg cggcgggcag ggagatgtcc atcttcttct gctccttgct
     3661 cagcttcgag gtgaccatga aaaagatcag cagcaggaag gtgaggtcaa tcatgggggt
     3721 gatgttgaac cccacctcgt cttgtctggc ttttccgcgg cgcatgatga ctggctttag
     3781 atgcgccgct tccgccgtcc ccgcaaacgg aaagctcgac acatttcagg ccccgtcatc
     3841 cccggaccgc cgccgcgcgg cccgccctca gcgcccctcg aaacgcagca ccaccttccc
     3901 ggaatccagc acccgcgtcc catccggcag cgtgccgcgg aattccttcg gcacctggag
     3961 cgccgcctcc ctcacacctt gatggaaaag acaggcatgg gcgtggtttt tgtctcctcg
     4021 atctcagggt actccccttc cccgggcggg ccggggaaat tctcgtccac aaagcgccgc
     4081 caggtgtctt tggtcttcgc ggaaagcagt tcctgcacgc catccgcgca ttggccgcag
     4141 atcatgatgc tgtgcagcag gcggtcatgc tcgcaaacac cggtgatgac atagtcgtcc
     4201 gtgccctcgc catcccgccc ctgcccgcag acggagcaga ggtggagtcc gcgctccagg
     4261 tccacgcttt cgtcctgaaa ggtggaaagg cgctttttcg actcctggga gaactcctcc
     4321 atcatggccg tgcggcagtg gtcgcagagc gcgtattcca tcacgcattc gccgttttta
     4381 taggccttgg agacttggaa tccgccggcg tcatcctcca gtgtctcgcc gcagcgcgtg
     4441 caatgcttga atgggcgctc ctcgtactcg caatgaagat cgcggggaat gggcggcggg
     4501 ctgggttcat ccatgggcga acatgaacgc gccctgccgt tttcacaagc gccatgcggg
     4561 gaggcaacat gagggctttt tctcacatga aggtgtttcc gcgtgccaga aagcgtggcg
     4621 gggcgtaact ctgcgcgtgc aaagccccgc ccccacctct ttgaccccgc actcccaggc
     4681 cgccctcatt ctggcgggcc acggctcgac cgtgaatccc gactccagcg ctcccactca
     4741 ttcgcacgcg gaccgcatcc ggcgcatggg ggtcttccgc gaggtggtgt gctgcttctg
     4801 gaaggaggag ccgggaatgc gggaggtctt cgagtcggtg gacagcgcgg aggtttatgt
     4861 ggtgccgaat ttcatcagcg aaggctattt ttgccagcag gtgctgccgc gcgagctggg
     4921 gctggacgga ccggtgacgc ggcgggaggg aaaagtggtt tactactgcg atccggtggg
     4981 agtccaccca agcatgacgc gcctgctgct gcgcagggct gatgaggtgg cgccgggcgt
     5041 gccgcgcgcg gaaacgagcc tggtggtggc gggccacggc acaagcctca atgagaactc
     5101 gcgcaaggcc atcgaaaatc aggtgcgcct gatccgtgag ggcgggcatg gctttgcgga
     5161 ggtggtggac gcttacatgg aggagccgcc cctggtgtcg gactgggcgg agctg
//
LOCUS       DAOMYJ010000301         5166 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Prosthecobacter sp. isolate AMR_MDS_5629
            NODE_5554_length_5166_cov_20.311485, whole genome shotgun sequence.
ACCESSION   DAOMYJ010000301 DAOMYJ010000000
VERSION     DAOMYJ010000301.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867850
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prosthecobacter sp. (wastewater metagenome)
  ORGANISM  Prosthecobacter sp.
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Verrucomicrobiaceae; Prosthecobacter.
REFERENCE   1  (bases 1 to 5166)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5166)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.30
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:34:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,742
            CDSs (total)                      :: 4,684
            Genes (coding)                    :: 4,676
            CDSs (with protein)               :: 4,676
            Genes (RNA)                       :: 58
            tRNAs                             :: 55
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5166
                     /organism="Prosthecobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5554_length_5166_cov_20.311485"
                     /isolate="AMR_MDS_5629"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1965333"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(296..1711)
                     /locus_tag="PLP58_20435"
     CDS             complement(296..1711)
                     /locus_tag="PLP58_20435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009966061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HRJ10962.1"
                     /translation="MSQPAANRRQFIFRTTLATAGALGFPAIVSSRSPNARVNLAFIG
                     VGGRGAANIFGLTGVPLAPRKDPKADPKAPPPPPPEPTENVVAICDVNAMNLDKAAQF
                     FPKARACRDFRELYDTSKDFDAVVVSTTEHTHAFATLPALLMKKPVYCEKPLTRDVAE
                     ARLITAAAEKAGVATQMGTQIHGMPNYRRVVELVQSGAIGAVSEVHVCVSRAWGLQSQ
                     EDAEKNKDVVFVTERPKEEQTPPPYLDWDLWLGPAPWRPYHEIYFPGPKWYRWWDFGN
                     GTMSDLGSHWNDLPFWALNLDAPLSVEAFGPEPHPEIAPATMSAVYEYGPRDGRPALK
                     LHWHQGATKPEVWKNDPVISQWNSGVLFIGERGRILSDYNKHLLLPEEKFQDFQPPKP
                     FIADSPGQHVEWLNAIKNGTPTGSPFSYAGPLSEANHLGNVAFRAGGKILWDAQAMRI
                     TNNESANRFLSREPRKGWSLG"
     gene            complement(1856..2953)
                     /locus_tag="PLP58_20440"
     CDS             complement(1856..2953)
                     /locus_tag="PLP58_20440"
                     /inference="COORDINATES: protein motif:HMM:NF024488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exo-alpha-sialidase"
                     /protein_id="HRJ10963.1"
                     /translation="MKSLILISSLAAPILFAAAPAVVVSEFIYDTGPYPQIHASTIVE
                     TPDGLVAAWFGGTHEKNPDVGIWVSRHVDGRWTESIEVANGVQHTLADGTVVRHPTWN
                     PVLFQPKDGPLLLFYKAGPNPQSWWGMLTTSTDQGKTWDQPRRLPEGILGPVKNKPVQ
                     MADGSILCPTSEETKAASKDEKETWTVHFERTRDFGRTWERTPPLHDGREIQAIQPSI
                     LRLGGDNLLALGRSRQDRVFEIRSSDGGKSWGEIKLGTLPNNNSGTDAVTLADGSHLI
                     IYNHIGGTPGKWGGKRTPLNLASSKDAQTWNAALVLESDPGEYSYPAIIQTRDGLVHV
                     TYTWKRQKVKHVVINPAKLESKPIVDGQWPQ"
     gene            complement(3075..3674)
                     /locus_tag="PLP58_20445"
     CDS             complement(3075..3674)
                     /locus_tag="PLP58_20445"
                     /inference="COORDINATES: protein motif:HMM:NF014316.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase"
                     /protein_id="HRJ10964.1"
                     /translation="MAHLGTDFFERDVLAVARDLVGAELVWQGCSGVIVETEAYAVEG
                     DPACHTASRPSAREFVSERPPGAAYVYFNYGMHWLFNLLVKGGGRDGLILIRALDPRR
                     GIAEMKKRRGRDKIEDLCSGPGKLALALGIGGGHHGTPLAGRGRPAGVGLRPPAGPLD
                     VLADVRVGISKAADFPWRFLLAGSPHVSVPHGRVKPWKA"
     gene            complement(3680..4438)
                     /locus_tag="PLP58_20450"
     CDS             complement(3680..4438)
                     /locus_tag="PLP58_20450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009965889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0051540 - metal cluster binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HRJ10965.1"
                     /translation="MDLHELVKHLNSTLRVAELPDYGSAVNGLQLASRNGSVSKVAAA
                     VDATLPVVRKAIAEGADLLIVHHGMFWSGLQPWTGAAFERMSAAIEAGLAIYSAHLPL
                     DAHPDLGNNARIAAALGLTPSGGFLNYKGLDVGVTCAADLTVEEVTARFSRALDGGRV
                     HVCAGGPQICRRIGISSGGSGSEVAAAAAAGVDTFLTGEGPHWSYTLAEELGINVLYG
                     GHYATETFGVKALAMHLEEKFGLPWRFVDHPTGM"
     gene            4572..>5166
                     /locus_tag="PLP58_20455"
     CDS             4572..>5166
                     /locus_tag="PLP58_20455"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HRJ10966.1"
                     /translation="MTFLRHNLFWITLALICAGYLLFWALLIGATAGYSTPEHWMKAL
                     SAPAIRHAGWLSLATSTGAALLALAIALPIGYLLARKDFKGKALLDAALDIPIVLPPM
                     VVGLCLLIFFQTTLGRVIEHGVEKGQLISVFGWFEFASPLKLPGFMVFTYTVAGVVLA
                     QFTVAAAFAIRTMRGTFDHLPARPEDVALTLGCTPGQA"
BASE COUNT          821 a         1587 c         1762 g          996 t
ORIGIN      
        1 gtgccaagct ccgaacaact tccacaggag aacggctgct tgattcttga gaattgccag
       61 ttccaacttc cttcctgaat gcgcgaagcc tgaaactcct gagttggcgg cgaaagactc
      121 tctaccaatg gtttaggcaa ctgtttcgat gacggagaca agagcctgtc agaacgagga
      181 gggtgtccga acgtttgtgt gcgccgcaag tgctcggcgc agcggcagaa ccgcgcgcac
      241 tttcaggaat gcacggccct tcgttgaccc cttgacggcg cagccgcctg gaccttcacc
      301 ccaggctcca gcctttgcgc ggctcgcggc tgaggaagcg gttggcggac tcgttgttgg
      361 tgatgcgcat ggcttgggcg tcccagagga ttttgcctcc ggcgcggaag gcgacgttgc
      421 cgaggtggtt ggcctcgctc agcgggccgg cgtagctgaa ggggctgccg gtgggggtgc
      481 cgtttttgat ggcgttgagc cactcgacat gctggccggg ggagtcggcg atgaagggct
      541 tgggcggctg gaagtcttgg aacttctcct cgggcagcag gaggtgcttg ttgtagtcgg
      601 agaggatcct gcctctctcg ccgatgaaga gcacgccgct gttccactgg ctgatgacgg
      661 ggtcgttttt ccacacttcg ggcttggtgg cgccctggtg ccagtggagc ttgagggcgg
      721 ggcgcccgtc gcgcgggccg tattcataga cggcgctcat ggtggcgggg gcgatctcgg
      781 ggtgcggctc ggggccgaag gcttccacgc tgagcggggc gtcgaggttc agcgcccaga
      841 agggcaggtc attccaatgg ctgccgaggt cggacatggt gccgttgccg aagtcccacc
      901 agcgatacca cttcgggccg gggaaataaa tctcatggta gggccgccac ggcgcggggc
      961 cgagccacag gtcccagtcc agataaggcg gcggggtctg ctcctccttg gggcgttcgg
     1021 tgacaaagac gacatccttg tttttctccg cgtcctcctg gctctggagg ccccaggcgc
     1081 gcgagacgca gacatggacc tcgctcaccg cgccgatggc cccgctttgc acgagttcga
     1141 cgacgcggcg gtagttcggc atgccgtgaa tctgcgtgcc catctgggtg gcgactccgg
     1201 ctttctccgc ggcggcggtg atgagccgcg cctcggccac atcgcgcgtg aggggtttct
     1261 cacaatacac gggcttcttc atgagcagcg cgggcagggt tgcgaaggcg tgcgtgtgct
     1321 cggtggtgct gacgacgacg gcgtcgaagt ccttcgacgt gtcataaagc tcgcggaagt
     1381 cgcggcaggc gcgcgctttg gggaaaaact gcgcggcttt gtcgaggttc atcgcgttca
     1441 cgtcgcagat ggccacgacg ttttccgtcg gctcaggcgg cggtggcggc ggggctttcg
     1501 ggtcggcctt ggggtcttta cggggtgcca gaggaacgcc ggtgaggccg aaaatgtttg
     1561 ccgcgccgcg cccgcccacg ccgatgaaag cgagattcac cctggcattc ggcgaacggg
     1621 agctgacgat ggccgggaag ccgagcgcgc cggcggttgc gagagtagtg cggaagataa
     1681 attgacggcg gttggcggct ggctgggaca tgggcaggga acgcggcgcg ggcgcggggt
     1741 cttgccggcg ctggtgttca ggctccggcg ctccaggctt cgctgaagtt ttgaaaacac
     1801 gcggactatg ggtggaaaat cgaaaatcga atttcgagct tcgaattttc cccgctcact
     1861 gcggccactg gccgtccacg atgggtttcg actccaactt cgcgggattt atgacgacgt
     1921 gtttcacttt ctgccgcttc caggtgtagg tgacgtggac gaggccgtca cgggtctgga
     1981 tgatggcggg gtagctgtac tcgccgggat cgctctccag cacgagggcg gcgttccagg
     2041 tttgcgcgtc tttgctggat gcgaggttca gcggcgtacg cttgcctccc cattttcccg
     2101 gtgtgccgcc gatgtgattg tagatgatga ggtggctgcc gtctgccagc gtgacagcat
     2161 cggtgccgct gttgttgttg ggcagggtgc cgagtttgat ttcgccccag cttttgccgc
     2221 cgtcgctgct gcggatttcg aagacgcggt cctgccgtga gcgtcccagg gcgaggaggt
     2281 tgtcaccgcc gaggcggagg atgctgggct ggatggcctg gatttccctg ccgtcatgca
     2341 ggggaggtgt gcgctcccag gtgcggccaa agtcgcgcgt gcgctcgaaa tggacagtcc
     2401 aggtttcctt ttcatccttg cttgcggcct tggtttcctc gctggtgggg cagagaatgc
     2461 tgccgtcggc catttgcacg ggcttgtttt tcaccggacc gaggatgcct tcgggaaggc
     2521 ggcggggctg gtcccaggtc ttgccttggt cggtggaggt ggtgagcatg ccccaccagg
     2581 actgcgggtt ggggcctgct ttgtaaaaga gcagcagcgg gccgtccttg ggctgaaaaa
     2641 gcacggggtt ccaggtggga tggcgcacca cggtgccgtc ggcgagggtg tgctggacgc
     2701 cgttggcgac ttcgatgctt tccgtccacc tgccatccac atgacgggag acccagatgc
     2761 cgacgtcggg attcttttca tgcgtgccgc caaaccacgc ggcgacaagg ccgtccggtg
     2821 tctcgacgat ggtgctggcg tgaatctgcg gataaggacc ggtgtcatag atgaactcgc
     2881 tgacaaccac ggcgggggcg gcggcgaaga ggatgggcgc ggcgagagaa ctgatcagga
     2941 tgaggctttt catggcgggc gggttggaga ggctggcagc ccaccgcaca gcacgcgggc
     3001 attcgagtca acgcccggcg tctgcgtatt tgcggacagc agatgcggat tcttgaccct
     3061 gtgcctgcca cgactcaggc cttccagggc ttcaccctgc catgcggcac gctgacatgc
     3121 gggctgcccg cgagcaggaa gcgccaggga aagtccgccg ccttgctgat gcccacgcgc
     3181 acatccgcaa gcacatccaa ggggccggcg ggcggccgca atccgacgcc cgccgggcgt
     3241 ccgcgccctg ccagaggagt gccgtggtgc ccgccgccga tgccgagagc cagcgccagc
     3301 ttgcccggac cggagcaaag gtcctcgatc ttgtccctgc ccctgcgctt tttcatttcc
     3361 gcgatgccgc gccggggatc aagcgcgcgg atgaggatga ggccgtcccg cccgccgcct
     3421 ttgaccagca ggttgaacag ccagtgcatg ccgtaattga agtaaacata agccgcgcca
     3481 ggcgggcgct cgctcacaaa ctcgcgcgcg ctggggcggc tggccgtgtg gcaggcgggg
     3541 tctccctcca cggcgtaggc ctccgtctcg acgatgaccc ccgagcagcc ctgccagacc
     3601 agctccgctc ccacaagatc acgcgccaca gcgagcacat ccctctcaaa gaaatcggtg
     3661 cctaaatgtg ccatgaccat cacattcccg tcggatggtc cacaaagcgc cagggcaggc
     3721 cgaatttctc ctcaaggtgc atggccagcg ccttcacgcc gaaggtctcg gtggcgtagt
     3781 ggccgccata gagaacgttg atgcccagtt cctcggccag ggtgtaactc cagtgaggcc
     3841 cttcgcccgt gagaaaggtg tccactccgg cagcggcggc ggcggccacc tcggagcccg
     3901 agcctcctga ggaaatgccg atgcgccggc agatctgcgg acctccggcg cagacgtgga
     3961 cgcgcccgcc atcgagcgcg cgggagaagc gcgcggtgac ctcctccact gtcaagtccg
     4021 ccgcgcaggt gacaccgaca tcaaggccct tgtaatttaa aaatccgccg cttggggtga
     4081 ggccgagtgc tgcggcgatg cgcgcgttgt ttcccagatc aggatgcgcg tcgagaggca
     4141 gatgcgcgct gtagatggcg agaccggcct cgatggccgc gctcatgcgc tcaaaggccg
     4201 cgccggtcca gggctgcaac ccgctccaaa acatgccgtg gtgcacgatc agcaggtcag
     4261 ccccctccgc gatggctttg cgcaccacgg gcagggtggc atccacggcg gcggccactt
     4321 tgctgacgct gccgttccgg ctggcaagct ggaggccgtt gaccgcgctg ccgtagtccg
     4381 gcagctctgc cacacgcaga gtggagttca ggtgtttgac gagttcgtgg aggtccatgc
     4441 aagttggggg aaagggggga tgactggcgg cggcggactg tcatgcctca atttgcattt
     4501 tcaatttcca agaggccgcg ccgtttgaca tccgccctgc cagcctttca ggttcgcaca
     4561 ccccggtcgc cgtgaccttc cttcgccaca atctcttctg gatcaccctc gccctcatct
     4621 gcgcgggcta tctgctgttc tgggcgctgc tcatcggagc aacggcgggc tacagcacgc
     4681 ctgagcattg gatgaaggcg ctctccgcgc ccgccatccg ccacgccggc tggttgagcc
     4741 tcgccaccag cacgggggcc gcactgctgg ctctcgccat cgccctgccc attggctacc
     4801 tgctggcgcg gaaggacttc aaaggcaagg cgctgctcga tgccgcgctg gacatcccca
     4861 tcgttctgcc gcccatggtc gtaggcctgt gcctgctcat ttttttccag accacgctgg
     4921 gccgcgtcat cgagcacggc gtggagaaag gccagctcat cagtgtcttc ggctggtttg
     4981 agttcgcctc gccgctgaag ctgcccggct tcatggtctt cacctacacc gtggccggcg
     5041 tggtgctggc gcagttcacc gtggccgccg cctttgccat ccgcaccatg cgcggcacct
     5101 tcgatcacct gcccgcgcgc ccggaggatg tggcgctcac cctcggctgc acgcccgggc
     5161 aggcct
//
LOCUS       DAOMYJ010000302         5161 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Prosthecobacter sp. isolate AMR_MDS_5629
            NODE_5564_length_5161_cov_16.452409, whole genome shotgun sequence.
ACCESSION   DAOMYJ010000302 DAOMYJ010000000
VERSION     DAOMYJ010000302.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867850
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prosthecobacter sp. (wastewater metagenome)
  ORGANISM  Prosthecobacter sp.
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Verrucomicrobiaceae; Prosthecobacter.
REFERENCE   1  (bases 1 to 5161)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5161)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.30
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:34:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,742
            CDSs (total)                      :: 4,684
            Genes (coding)                    :: 4,676
            CDSs (with protein)               :: 4,676
            Genes (RNA)                       :: 58
            tRNAs                             :: 55
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5161
                     /organism="Prosthecobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5564_length_5161_cov_16.452409"
                     /isolate="AMR_MDS_5629"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1965333"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            362..434
                     /locus_tag="PLP58_20460"
     tRNA            362..434
                     /locus_tag="PLP58_20460"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:395..397,aa:Arg,seq:tct)
     gene            523..1827
                     /locus_tag="PLP58_20465"
     CDS             523..1827
                     /locus_tag="PLP58_20465"
                     /inference="COORDINATES: protein motif:HMM:NF012798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HRJ10967.1"
                     /translation="MKSPEIATGGRGQTLQRRKRAAVPKIVEQRNSSGTVRWMISGLY
                     VDGKRKREFFTDPDTAKRRLQMLQTARANTGALAERIAKRPELAADAGRAADLLAPLG
                     LSLLDAARVHVECLKALEGTGLALLDVARDAAAAHASRRAALTLEALGARFLSDPETL
                     KLSRAYLVDVRNRWRRFQDDMGAETLACDVTPDRVRRWLAGLDVAAISKGNFHRTCSA
                     VFSYAMHAELVRENPFRKVKKPVVETKDGVEVFTPEQMGALLRAADQSWLPALAIGGF
                     AGLRPEELRRLEWDEVDLKAGFIEVKASKSKTGRRRLVTISANLLSWLEPCAREKGRV
                     VNPNERKKRLAAMKAAGIERWPSDVLRHSFASYHLAAHKDMNGTALELGHNSTTMLFR
                     HYREAVKPEAARAWWALMPEKCGNVLAFPAGKTGRKKKGAAA"
     gene            1869..2177
                     /locus_tag="PLP58_20470"
     CDS             1869..2177
                     /locus_tag="PLP58_20470"
                     /inference="COORDINATES: protein motif:HMM:NF021431.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HigB family
                     toxin"
                     /protein_id="HRJ10968.1"
                     /translation="MRVIKPATVREWMRQHPTAKPGLEWWLDTVRCGGWGSLAELRRT
                     FPAADLVRVASGRKVIVFNIGGNAFRLVAAVHFNTGLAYALAFMTHAEYSKDRWKETL
                     "
     gene            2174..2617
                     /locus_tag="PLP58_20475"
     CDS             2174..2617
                     /locus_tag="PLP58_20475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HRJ10969.1"
                     /translation="MKTRTTSPHRSPARLPEDYASLCRKVWLPRPIHDAQEHAAALAA
                     IEPLWGREEEMNEDQADWFKLAADLIAEYEERAAPKRKPLPLARRLAGLLEAHGMTAA
                     DLGRLLELEPSMGSKIMNGARQLTAAHVRKLARHFALPADYFLEP"
     gene            2614..3801
                     /locus_tag="PLP58_20480"
     CDS             2614..3801
                     /locus_tag="PLP58_20480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ10970.1"
                     /translation="MKKKAARKKAQPAKSAGGGGDARRRKTRGPDMDSGAGNFSQGSG
                     DGAGKVWRGWIHNEEGKMIAKIEALSADDLDAALEGINAGGRIIQEAKAWNMKPEDYW
                     QLENFPSNPDDWEVRMQRLIASKLVDFNSDTDGLMHSCTWIIDLLWKWLHEVAVKTEN
                     PNHKRDAGRTLGRLYHQARQEGEEERLSSANEEFRSNINPFPWARRPPSKALVNWTNR
                     KMHRHLEFWKKAVEVAAAFEDKKPFSRDDRDSQGVHHFQRLPMMDMKEAWQSYFIQER
                     NAYPGRSGSWSDFLDELEIHPFRKRMLALSQLTAFKECWKLALYDVIKEEWNKDLEIK
                     KACGSKFVGRFGDKTHLEGAGFHLAEDFFKRSYLPQWNQKKRLKSMMEQMIRCRKKQA
                     VEG"
     gene            3946..4254
                     /locus_tag="PLP58_20485"
     CDS             3946..4254
                     /locus_tag="PLP58_20485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ10971.1"
                     /translation="MDNTSNTERPRRKALVKPGPAKAGTGNKNNETAPRRRRTGGGEL
                     LKNRREMAQAAGFSMRTLDRLTKARVVPVVRIGRLCFYDPARVMAALQRNCEQKEVQP
                     "
     gene            4251..4601
                     /locus_tag="PLP58_20490"
     CDS             4251..4601
                     /locus_tag="PLP58_20490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ10972.1"
                     /translation="MMPQHFQEVRETDEAEAANRLLAEGWALLGVFQRAEVCDGRLLC
                     WALYVLGRGEAGQGVEAIEHVEEVAKDAANKALAEGAVLMRVITRKADEEEYPRFIIG
                     WPKRGGGFGNNGVH"
     gene            4900..>5161
                     /locus_tag="PLP58_20495"
     CDS             4900..>5161
                     /locus_tag="PLP58_20495"
                     /inference="COORDINATES: protein motif:HMM:NF013930.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CHC2 zinc finger domain-containing protein"
                     /protein_id="HRJ10973.1"
                     /translation="MKDAFTVADAWHALGLEGKPAKSCRCPWRNDRRPSFSVWAEGRR
                     WHDHASGEGGDVVDFVKAAAGLETAEAIRGWTSQYIIAQPRIL"
BASE COUNT         1113 a         1371 c         1779 g          898 t
ORIGIN      
        1 cacccgccac cgaggctcca aatccacccg tggtccagca acctgcgcca atgcccgccc
       61 ccgcccccat gatgacacca ccctccgaag agtcaggttt tcaaacttat ctgatcattg
      121 gcatgggcgt ggtgcttctc cttctggtgg tcatgctgct tcagaagaaa aagtgagcct
      181 ccctcagtct cccgccggac gcccgcaagg gtgtccggct ttttgttgtc cggcgcaggc
      241 cggcaaaccc gcgcgaacaa gcgcctccca cccatgcgtg aagcctggaa catgcccgtc
      301 ccctctggca gaagcaggct tgcccggcac tgataacgcc ttatgatgct cacaccacga
      361 cgcgcccgta gctcagtgga tagagcagcg gatttctaat ccgctggtcg ttggttcgat
      421 cccaaccggg cgtgctctca taatcaatga gttacgaaga ttttagctta ttgagaatga
      481 aagacattgc cagataggtg acagttaagc tagtttcggg gcatgaaatc gccagaaatt
      541 gccacgggtg gacggggaca gacattgcag cggcgcaagc gcgcggcggt gccgaagatc
      601 gtcgagcaac gaaattcttc cgggacagtg cgctggatga tctccgggct ttatgtggac
      661 gggaaacgca agcgggagtt cttcacggac ccggacacgg ccaagcgccg cttgcagatg
      721 ctccaaacgg cgcgggccaa tacgggggca ctggcggaac gaatcgccaa gaggccggag
      781 ctggcggcgg atgctggccg ggcggcggat ttgctggctc ccttggggct ttccctgctg
      841 gatgcggcgc gggtgcatgt ggaatgcctc aaggcgctgg aaggcacggg gctggcgctg
      901 ctggatgtgg cgcgggacgc ggcggcggct catgcctcac gccgggcggc gctgactctg
      961 gaggcgctgg gcgcgcgctt tctctccgac cctgagacgc tgaaactttc cagggcgtat
     1021 ctggtggatg tgcgcaaccg ctggcggcgc ttccaggatg acatgggcgc ggagacgctg
     1081 gcttgtgatg tgacgccgga cagggtgcgg cgctggctgg ccgggctgga tgtggcggcc
     1141 atctcaaagg ggaacttcca tcgcacttgc agcgcggttt tcagctatgc catgcacgcg
     1201 gagctggtgc gggagaatcc tttccgcaag gtgaagaagc cggtagtgga aacaaaggac
     1261 ggtgtggagg tcttcacgcc ggagcaaatg ggggcgctgc tgcgggcggc ggaccagtca
     1321 tggcttccgg cgcttgccat cggcggcttt gcgggcctga ggccggagga actgcggcgg
     1381 ctggagtggg atgaggtgga cttgaaggcg ggcttcatcg aggtgaaggc gagcaagagc
     1441 aagacgggca ggcggcggct ggtgacgatc tccgcgaatc tgctttcctg gctggagcct
     1501 tgcgcgcggg agaagggccg ggtggtgaat ccaaacgagc gcaagaagcg gctggcggcc
     1561 atgaaggcgg cggggattga gcgctggccg tcggatgtgc tgcggcactc cttcgccagc
     1621 tatcatctgg cggcgcacaa ggacatgaac ggcacggcgc tggagctggg ccacaattcg
     1681 acaaccatgc ttttccggca ctaccgggag gcggtgaagc cggaggcggc gcgggcgtgg
     1741 tgggcgctga tgccggagaa gtgcgggaac gtgctggcct tcccggcggg gaagacgggc
     1801 cggaaaaaga agggcgcggc ggcatgaatg cggctggaca ttttgtccag ccaagtccat
     1861 cctagtccat gagagtcatc aagcctgcca cggtgcgcga gtggatgcgg cagcatccga
     1921 ccgcaaagcc gggcctggaa tggtggctgg acacggtgcg ctgcggcggc tgggggagcc
     1981 tggccgaact gcggcggacg tttccggcgg ctgatctggt gcgcgtggcg agcggcagga
     2041 aggtgattgt cttcaacatc ggcggcaatg ccttccggct ggtggccgcc gtgcatttca
     2101 acacggggct ggcctatgcg ctggccttca tgacacacgc cgaatactcg aaagaccgct
     2161 ggaaggaaac gctatgaaaa cccgcacgac atccccgcac cgttccccgg ccagactgcc
     2221 ggaggattac gccagcctgt gccgcaaggt ctggctgccg cgccccatcc acgacgcgca
     2281 ggagcacgcg gcagcgctgg cggccatcga accgctttgg ggccgtgagg aggagatgaa
     2341 cgaggatcaa gcggactggt tcaagctggc ggcggacctt atcgccgagt atgaggagcg
     2401 cgccgcgccg aaaaggaagc cgctgccgct ggcgcggcgg ctggccgggc tgctggaggc
     2461 gcacgggatg acggcggcgg acctggggcg gctgctggag ctggagccga gcatgggcag
     2521 caagatcatg aacggggcgc gccagctcac ggcggcgcat gtgcggaagc tggcgcgcca
     2581 cttcgcgctt ccggcggatt acttcctgga gccatgaaga agaaggcggc caggaagaaa
     2641 gcgcaacccg ccaagtctgc cgggggcggg ggggatgcgc ggcgacgcaa gacgcgcggc
     2701 cctgatatgg attcaggggc gggaaatttt tcacaaggca gcggagatgg agccggaaaa
     2761 gtgtggcggg gctggattca taacgaagaa ggcaagatga ttgcaaaaat cgaagctctt
     2821 tctgccgatg atctggatgc tgctttggaa ggcatcaatg cggggggcag aatcattcag
     2881 gaagcaaagg cttggaacat gaagcccgaa gactactggc agcttgaaaa tttcccatcc
     2941 aacccagatg actgggaggt gagaatgcaa aggctcatcg ccagcaagct ggtggatttc
     3001 aattctgaca cagatggcct gatgcactct tgtacatgga tcattgatct gctgtggaaa
     3061 tggcttcatg aagtggcggt caaaactgaa aatccaaacc acaagcgtga cgctggcagg
     3121 actctcggca gactctatca ccaagcaaga caagagggcg aggaggaacg attatcgagt
     3181 gccaatgagg aatttcggag caatatcaat cctttcccgt gggcaagaag accgccttcc
     3241 aaggcgcttg taaactggac caatcgaaag atgcataggc atctcgaatt ttggaaaaag
     3301 gcggtagagg tggccgcagc ctttgaggac aagaaacctt tcagccgaga cgaccgcgat
     3361 tcccaagggg tgcatcattt tcaacgtctg ccgatgatgg acatgaagga ggcttggcag
     3421 agctacttca ttcaagaacg caacgcgtat ccaggacgat ccggttcatg gagtgatttt
     3481 ttggatgaac ttgaaatcca tcctttccgc aaacgaatgc tagctttatc gcaactgaca
     3541 gcattcaagg aatgctggaa gctggcttta tatgatgtga tcaaagaaga atggaacaaa
     3601 gacctcgaaa tcaagaaggc gtgtggatca aaatttgtgg gtagatttgg cgacaagacg
     3661 catttggaag gcgctggatt tcatctggca gaggatttct tcaagcggtc ttacctgcct
     3721 cagtggaatc aaaagaagcg cctgaaatcc atgatggagc agatgatacg atgccgaaaa
     3781 aagcaagctg ttgaagggtg atcccggaac aaaaagtctc cgcacaagca ttcaaaccac
     3841 atagtcaagc acgggcgaat ttaagcctaa ttcgcccaag atggcgcacg catgaaattg
     3901 catcgaggtg ccagcaatcg ccagaaaaac cctggcggac attgtatgga taacacctca
     3961 aacactgaaa ggccgcgccg gaaggcgctg gtgaaacccg gaccggccaa agccgggacc
     4021 ggcaacaaaa acaacgaaac cgcaccacgg cggcggcgga caggcggcgg cgagctgctg
     4081 aagaaccgcc gcgagatggc acaggcggcg ggcttttcca tgcggaccct ggaccggctg
     4141 acgaaggcgc gggtggtgcc ggtggtgcgc atcggcaggc tgtgcttcta tgatccggcg
     4201 cgggtcatgg cggcgctgca acgcaactgc gaacagaagg aggtgcagcc atgatgccgc
     4261 agcacttcca agaagtccgt gagacggacg aagccgaagc cgccaacagg ctgctggcgg
     4321 agggctgggc gcttctgggc gtctttcagc gggccgaggt ctgcgatggc cgtcttttgt
     4381 gctgggcgct gtatgtcctg gggcgagggg aagccgggca gggcgtggaa gccatcgagc
     4441 acgttgagga ggtggcgaaa gacgctgcca ataaggcgct ggctgaaggt gcggtgctga
     4501 tgcgtgtcat cacacggaag gccgacgagg aggagtatcc gcgtttcatt atcggctggc
     4561 cgaaacgcgg cgggggcttt ggcaacaatg gagttcactg aggggagagg gcggaacagg
     4621 cggaacccat gaaggagaga agaaggactt cttctctttt cccggagtcc ctggccgtgt
     4681 ccggcgcggt ttttcccgga ggcgcattcc ccgccctgtc tgtcacctgc tggcgagtgc
     4741 tggcacggcg gcaggcaggg gcgggattcc tcctccggca tggctgaaag caggcatgga
     4801 ctgagggctt gaggccgggg catgggctgg ctcctcctcc gcttttcccc cgcaaccgga
     4861 ggaatgaaga tgatgaatgt acgaggtttg attgaacggg tgaaggatgc cttcacggtg
     4921 gcggatgcct ggcacgcgct gggcctggag ggcaaaccgg ccaagagctg ccgctgcccg
     4981 tggcgcaatg accggaggcc gagcttttcc gtatgggcgg agggccgccg ctggcatgac
     5041 cacgccagcg gcgagggcgg ggacgtggtg gattttgtga aggcggcggc agggctggag
     5101 acggcggagg caatcagggg atggacatcc cagtacatca tcgcgcagcc caggatcttg
     5161 a
//
LOCUS       DAOMYJ010000303         5148 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Prosthecobacter sp. isolate AMR_MDS_5629
            NODE_5589_length_5148_cov_19.993913, whole genome shotgun sequence.
ACCESSION   DAOMYJ010000303 DAOMYJ010000000
VERSION     DAOMYJ010000303.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867850
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prosthecobacter sp. (wastewater metagenome)
  ORGANISM  Prosthecobacter sp.
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Verrucomicrobiaceae; Prosthecobacter.
REFERENCE   1  (bases 1 to 5148)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5148)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.30
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:34:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,742
            CDSs (total)                      :: 4,684
            Genes (coding)                    :: 4,676
            CDSs (with protein)               :: 4,676
            Genes (RNA)                       :: 58
            tRNAs                             :: 55
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5148
                     /organism="Prosthecobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5589_length_5148_cov_19.993913"
                     /isolate="AMR_MDS_5629"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1965333"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            62..469
                     /locus_tag="PLP58_20500"
     CDS             62..469
                     /locus_tag="PLP58_20500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ10974.1"
                     /translation="MFDPSLPQENTPVDAAQMRAQLTGLKDLIDAVPAITSAVVDAVD
                     TLPPDESATVSVSVTGTVLHLTFGIPQGEQGDSGPPGEVSAQDLADGLETRAHAIPST
                     GTLDQSAEPEYSPTQAQDIINTLNALITALKGS"
     gene            524..1366
                     /locus_tag="PLP58_20505"
     CDS             524..1366
                     /locus_tag="PLP58_20505"
                     /inference="COORDINATES: protein motif:HMM:NF024762.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase-like domain-containing protein"
                     /protein_id="HRJ10975.1"
                     /translation="MSALANLPHLITLLDDESEVVREALQRELGSIRRELPEQLRLMR
                     RRLTPEEEGHLNRILDPLRQTDLENAWMRWRWMDDPTTQLEVGVAQISAYLHGWSCQP
                     ADLGRRLDALADEAFKDQGRMDARELAAWMFAFQHGGARFRGNNKDYYAPGNSDLFQV
                     LETGLGNPISLACLYRLLGQRFGIGIGGCNFPGHFLARVETDGGLFLVDCFNRGRFMP
                     AADVARHHPAATPGVEEIIREAASTEAVLLRILRNLDESCGRARDTRLRQLMRRLAVR
                     LMEE"
     gene            complement(1411..1629)
                     /locus_tag="PLP58_20510"
     CDS             complement(1411..1629)
                     /locus_tag="PLP58_20510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ10976.1"
                     /translation="MTPSKLKTSPDRTWTWEQWQAGRAERRRVGNLVAPKNIRRAKSE
                     GYEYLRETYGGERGPKFNKPFDLSADEV"
     gene            complement(2201..2422)
                     /locus_tag="PLP58_20515"
     CDS             complement(2201..2422)
                     /locus_tag="PLP58_20515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ10977.1"
                     /translation="MTTAEITAALGIIRAETDLSAKALLLAGLVSELFRERGFEPVVV
                     GGSAIEFYTDGAYMSGDTDICWGCVARMM"
     gene            complement(2484..3230)
                     /locus_tag="PLP58_20520"
     CDS             complement(2484..3230)
                     /locus_tag="PLP58_20520"
                     /inference="COORDINATES: protein motif:HMM:NF026257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HRJ10978.1"
                     /translation="MSKRPQPRRLSGLLFRWEWKKTAAMSRQPRIIPVAALARSGYHS
                     TMSTVIALQPVSWQRMAKGIDDVRQRLERAAAALEKLGVQYAVVGGNAVAAWVSRVDE
                     SAVRNTRDVDILLRREDAEKAREALEAAGFVHRRVASLGKAGAMDVFLDGPDAKVRDA
                     VHVLWAGEKPVPDAIENTPELRETEQGEGFELVPLQDLVRMKLVSFRDKDRMHLRDLL
                     SVGLIDESWTGRFPKELAARLHVILNDPEG"
     gene            3233..4357
                     /locus_tag="PLP58_20525"
     CDS             3233..4357
                     /locus_tag="PLP58_20525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ10979.1"
                     /translation="MKSAPILLALAGVTALAATKPKPEFQYQSAGIAIPVPTADEPKA
                     AAFGPESVRAAARYMEDGALSWVREKSCVNCHTTGPYLSERPALTPWLGKPSEEVLAN
                     FIEAVPEQVKPQQETEKDGHRYYAGSWTSIWRSLGLAEWDRHVTGALSPHTDLSLREM
                     FERQSASGAFVSYGEVEIPHITTDFELTLQAARAVTAAPGWLEKQTDPALLARIGKMK
                     DWLRDAPPRNDFDRVLRLQLAHYFPGLVTPAQTGSALALLAAKQHADGGWSTRDFSAV
                     EDWHYKMSETVLKLIASLPDAARPESDPYMTALAIVLMRQSGVPAGDERVRRGIAWLK
                     GEQRASGRWWMHSLYRGNYHYITYIATAQALKALALCGEL"
     gene            complement(4391..>5148)
                     /locus_tag="PLP58_20530"
     CDS             complement(4391..>5148)
                     /locus_tag="PLP58_20530"
                     /inference="COORDINATES: protein motif:HMM:NF012493.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="C-terminal helicase domain-containing protein"
                     /protein_id="HRJ10980.1"
                     /translation="KIELKAFCTLTRRQATLYAKLVDQLAKMLDDKDLEPVKRQGLVL
                     GFLLKFKQVCNHPSHWNGDGVWQPEDSGKFARLTEICAQLAERRERALVFTQFQETCA
                     PLARHLAGVFGREGLILHGGTAVKKRPDLVESFQQPGGPPFMVISVKAGGTGLNLTAA
                     SHVIHFDRWWNPAVENQATDRAFRIGQKRNVIVHKFLCQGTIEERIDALIEEKLALSS
                     DLLGSGAGAEKLLTDMSGDELLDLVRLDVSAVG"
BASE COUNT          867 a         1757 c         1654 g          870 t
ORIGIN      
        1 tccgcattcc gcattccgca ttccgcattc cgcattccgc attccgcatt ccgcaattcc
       61 catgttcgac ccatccctcc cgcaggaaaa cacgcccgtg gatgccgcgc agatgcgcgc
      121 gcagctcacc gggctgaaag acctcattga cgcggtgccc gccatcacct ccgcggtggt
      181 ggacgcggtg gacacgctgc cgcccgacga atccgccacc gtgtcggtgt ccgtcaccgg
      241 cacggtgctg cacctgacct tcggcatccc ccagggggag cagggggact ccggaccgcc
      301 gggggaagtg agcgcgcagg acctagcgga cgggctggag acgcgcgcgc acgccatccc
      361 ctcgacgggc acgctggacc agtcggcgga gccggagtat tcaccgacgc aggcgcagga
      421 catcatcaac acgctcaatg cgctgatcac ggcgctgaag gggagctgac tcgttggcgc
      481 atttcagctt tgcagcgcgc gggctttccg ctaaaggagg ctcatgtccg cgctcgccaa
      541 cctgccgcac ctcatcacgc tgctcgatga tgagtccgag gtggtgcggg aggcgctgca
      601 gcgggagctg ggcagcatcc gccgggagct tcccgagcag ctccggctca tgcgccgccg
      661 gctgaccccc gaggaggagg gccacctcaa ccgcatcctc gacccgctgc gccagaccga
      721 cctggaaaac gcctggatgc gctggcgctg gatggacgac ccgaccacgc aactcgaggt
      781 gggcgtggcg caaatctccg cctacctcca cggctggagc tgccagccgg cggacctggg
      841 ccgccggctc gacgcgctgg cggatgaggc cttcaaggac cagggccgca tggacgcgcg
      901 cgagctggcc gcgtggatgt tcgccttcca gcacggcggc gcccgcttcc gcggaaacaa
      961 caaggactac tacgcgcccg gcaacagcga cctcttccag gtcctcgaaa ccgggctggg
     1021 caaccccatc agcctcgcct gcctctaccg cctgctcggc cagcgcttcg gcatcgggat
     1081 cggcggctgc aacttccccg gccacttcct cgcccgcgtg gagacggacg gcgggctttt
     1141 cctcgtggac tgcttcaacc gcgggcgctt catgcccgcc gccgacgtgg cccgccacca
     1201 ccctgccgcc actccgggag tggaggagat catccgcgag gccgcctcca ccgaggccgt
     1261 gctcctgcgc atcctgcgca acctcgacga atcctgcgga cgcgcgcgcg acacccgcct
     1321 gcgccagctc atgcgccgcc tcgccgtgag gctcatggag gaatgacccg gcggcagcct
     1381 cgccgccccc cccccgcgcg cgagccgctc tcacacctcg tcggcggaga gatcgaaggg
     1441 tttgttgaac ttcgggccgc gctcgccgcc gtaggtctca cggaggtatt catacccctc
     1501 gcttttcgcg cggcggatgt ttttcggagc cacgagattc cccacacggc gcctctccgc
     1561 gcggcccgcc tgccattgct cccaggtcca ggtgcggtcc gggctggttt tgagtttcga
     1621 aggtgtcatt tggaggaacc ctacattcag accgcccttt tgccaaatcg gcttccacct
     1681 cgcatctcat gtcttcaaat tccttccgac agttgtattt ggagctgctg acaatccggc
     1741 gcgcctcctc ccaatcaatt ggaatctgcc ccttcaaagc agatgccatc agctttttgg
     1801 cacaatcatc atctttctcg tcatagccgc tgtggtatcg tcttgcggca tacacacggc
     1861 ccgccaaagc atcttccaca gggatcaacc gcacctcgcc aaacggcgtg tcgaagcgag
     1921 aaaatgcctt ctcatagcgg cagtcaatct cccccggcaa atcaatccac agcccctcaa
     1981 tgccccagct tttccctccg ccgtggcatt tcacacctgg aatctggcgc atgatctcct
     2041 ccctttgccc gatggccggc tgaggcaagc cggcccagca gagggtgcgt cgccggaatg
     2101 atgtagcgta ggacgactgt ggctttagcc cggtcgtctg gtgggcgcac gatgcgaaac
     2161 gacgcctccc aaaagccggg cgggctgcgc cacacccggc ctacatcatt ctggcgacgc
     2221 acccccagca gatgtccgtg tcaccggaca tgtaggcgcc atccgtgtaa aactcgatgg
     2281 ccgagccgcc gacgaccacg ggctcaaaac ccctctcgcg aaacagctcc gacaccagcc
     2341 cggccagcag aagcgccttg gcgctcagat cagtctctgc ccggatgatg ccgagcgcgg
     2401 cggtgatttc agcggtggtc atgcgggtga ctgataactc ggaaccgcgc gtctgggaat
     2461 gctgaactgc aagcgcggcg gtttcagccc tcagggtcat tcagaatgac atggagccgt
     2521 gccgcgagtt ccttcggaaa gcgcccggtc cagctttcat caatgaggcc gacggaaagc
     2581 agatcgcgga gatgcatgcg gtccttgtcg cggaaggaga cgagcttcat gcgcacgagg
     2641 tcctgcaacg gcacgagttc gaagccttca ccctgctcgg tctcgcgcag ttcgggtgtg
     2701 ttctcgatgg cgtccggcac gggcttctcg cccgcccaga gcacatgcac ggcatcacgc
     2761 actttggcgt caggtccgtc gaggaagaca tccatggcgc cggctttgcc gagtgaggcg
     2821 acgcggcgat ggacaaaccc ggcggcttcg agggcttcgc gggctttttc ggcgtcttcg
     2881 cggcggagca ggatgtccac atcacgcgtg tttcgcaccg cgctctcatc cacacgggaa
     2941 acccaggccg cgacggcatt gccacccact acagcgtatt gaacaccgag cttctccagc
     3001 gcggcggctg cgcgttcgag gcgttgtctg acgtcgtcga tgccttttgc catgcgttgc
     3061 cagcttacgg gttgaagagc gatcacggtg ctcatggtcg agtgatagcc gcttcgggca
     3121 agggcggcaa ccgggatgat gcgcggctgg cgggacatgg cggcggtctt tttccactcc
     3181 catcgaaaca gcaggcccga tagccggcgc ggctgcggcc gtttactcac gcatgaaatc
     3241 cgcgcccatc ctcctcgccc tcgccggcgt caccgcgctc gccgccacga agccgaagcc
     3301 ggaattccag taccagagcg cgggcatcgc catccccgtc cccaccgccg acgagccgaa
     3361 ggcggccgcc ttcgggccgg agagcgtccg cgccgccgcc cgctacatgg aggatggggc
     3421 gctctcctgg gtgcgtgaaa agtcctgcgt caactgccac accaccggcc cctacctgag
     3481 cgagcgcccc gcgctcaccc cctggctggg caaaccctcg gaggaggtgc tggcaaattt
     3541 catcgaggcc gtgccggagc aggtcaagcc gcagcaggag accgagaagg atggccaccg
     3601 ttactatgcc ggctcgtgga cgagcatctg gcgctcgctc ggcttggccg agtgggacag
     3661 gcatgtcacc ggcgcgctca gcccgcacac ggacctctcc ctgcgcgaga tgttcgagcg
     3721 gcagtccgcc agcggcgcct tcgtcagcta tggcgaggtg gagatccccc acatcaccac
     3781 cgacttcgaa ctcaccctgc aggccgcgcg cgccgtgaca gcggctccgg gctggctgga
     3841 aaaacaaacc gaccccgcgc tgctcgcccg gatcggaaaa atgaaggact ggctgcgtga
     3901 cgcgccgccg aggaacgact tcgaccgcgt gctgcgcctg cagctcgccc attactttcc
     3961 cggcctggtc acgccggcgc agaccgggtc cgcgctggcc ctgctggcgg cgaagcagca
     4021 cgccgacggc ggctggtcca cgcgggattt ctccgccgtc gaggactggc actacaagat
     4081 gagcgagacg gtgctcaagc tcatcgccag cctgccggac gccgcgcgtc cggagagcga
     4141 cccctacatg accgcgctgg ccatcgtgct catgcgccag agcggcgtgc ccgccggcga
     4201 cgagcgcgtg cggcgcggca tcgcctggct caagggcgag cagcgcgcca gcgggcgctg
     4261 gtggatgcac tccctctacc ggggcaacta ccactacatc acctacatcg ccacggcgca
     4321 ggcgctcaag gcgctggcct tgtgcgggga gctttgaaag aagagcgcga acccgccgaa
     4381 gggcggcctt tcaacccacc gcgctgacat ccaggcggac gaggtcgagc agctcgtcac
     4441 cgctcatgtc ggtgaggagt ttttccgcgc ccgcgccgga gccgaggagg tcggaggaaa
     4501 gggcgagctt ttcctcgatg agggcgtcga tgcgctcctc gatggtgccc tggcagagga
     4561 atttgtggac gatgacattg cgcttctggc cgatgcggaa ggcgcggtcg gtggcctggt
     4621 tttcgacggc gggattccac cagcggtcga agtggatgac gtggctggcg gcggtgaggt
     4681 tcagcccggt gccgccggcc ttgacgctga tgaccatgaa gggcgggccg ccgggctgct
     4741 ggaagctttc gacaagatcg gggcgttttt tcacggcggt gccgccgtgg aggatgaggc
     4801 cctcgcgccc gaagacgccg gcgaggtggc gggcgagagg ggcgcaggtc tcctgaaact
     4861 gggtgaagac cagggcgcgc tcccgccgct cggcgagctg ggcgcagatt tcggtgaggc
     4921 gggcgaattt gccgctgtcc tccggctgcc agacgccgtc gccattccag tggctggggt
     4981 ggttgcagac ttgcttgaac ttgagcagga agccgaggac gaggccctgg cgcttgacgg
     5041 gttcgaggtc cttgtcgtcg agcattttgg cgagctggtc cacgagcttg gcgtagaggg
     5101 tggcctggcg cctggtgagg gtgcagaagg ctttgagttc gatcttgt
//
LOCUS       DAOMYJ010000304         5129 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Prosthecobacter sp. isolate AMR_MDS_5629
            NODE_5614_length_5129_cov_21.118250, whole genome shotgun sequence.
ACCESSION   DAOMYJ010000304 DAOMYJ010000000
VERSION     DAOMYJ010000304.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867850
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prosthecobacter sp. (wastewater metagenome)
  ORGANISM  Prosthecobacter sp.
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Verrucomicrobiaceae; Prosthecobacter.
REFERENCE   1  (bases 1 to 5129)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5129)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.30
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:34:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,742
            CDSs (total)                      :: 4,684
            Genes (coding)                    :: 4,676
            CDSs (with protein)               :: 4,676
            Genes (RNA)                       :: 58
            tRNAs                             :: 55
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5129
                     /organism="Prosthecobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5614_length_5129_cov_21.118250"
                     /isolate="AMR_MDS_5629"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1965333"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..348
                     /locus_tag="PLP58_20535"
     CDS             <1..348
                     /locus_tag="PLP58_20535"
                     /inference="COORDINATES: protein motif:HMM:NF012514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HRJ10981.1"
                     /translation="EQAAAREVREETGLHLPAVAFRPLATLPNEYEFQDYLWPTLDLF
                     YTVQAPENAALRPDPAEVSEALWLPPEDAAMCDFAFESNRRAVRLLLSARNQGPQTQG
                     EAHNLPHFCQNAA"
     gene            complement(531..845)
                     /locus_tag="PLP58_20540"
     CDS             complement(531..845)
                     /locus_tag="PLP58_20540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ10982.1"
                     /translation="MLRPNEIAEYGERHAEKWLAAQGYHCHHNQRQGVRDFEARGGES
                     QLLVHVNTAIAPGHPPEISQHDHDSIVSRAMMLGFEPWLAQVQIDKNGDLVGEIAWSH
                     MK"
     gene            complement(985..1743)
                     /locus_tag="PLP58_20545"
     CDS             complement(985..1743)
                     /locus_tag="PLP58_20545"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HRJ10983.1"
                     /translation="MDLQLKGQTAVVIGAASGIGRAIARGFEAEGCAVRGFDLRASEG
                     VAITTGDVTDYSALRAFAADCGAVDHVVFAAGAGSGKFGFPFWNLEPGDWQRVLEINL
                     VGAVNTAHAFAPALIARGGGTLLFLVSVAGQTGSQTDPPYSAAKAGLINFMQCAAKDL
                     APRDVRVNALSPGMVRTPLNEAVWAAAQARLPEAERQGYDSWAEEKMRKVSPLGRWQT
                     PEELAAMAVFLASPCARNITGQTLNVDGGQVMHA"
     gene            complement(1822..1898)
                     /locus_tag="PLP58_20550"
     tRNA            complement(1822..1898)
                     /locus_tag="PLP58_20550"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1862..1864),aa:Pro,seq:ggg)
     gene            2190..3260
                     /locus_tag="PLP58_20555"
     CDS             2190..3260
                     /locus_tag="PLP58_20555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ10984.1"
                     /translation="MAWKLLSILSAVCLGAACYFAHANKQRLENEKTRQAAAEANLKA
                     AQDRKAEGDAAVKVKEEQYAAIQKERDAAKEEVVKLAADAQEKEAALSVVKGNLEQVT
                     TQVNAVQKQIDDAGDIEKLIAQVEKLKKDQTEAEGAVANQKQRLANVQATIAGVTGQI
                     DKLKETEAQGRRGIVAPEFSARVAQFFPEWGFAILNKGNSQGVFANADLEVKRGKDVI
                     ARLKVRSVEQHNAIADVVPGSVAEGSSIFNGDSVVAAAQQSAADPKKGSSSTGGTPEQ
                     GAPVPDSTAPQAPAMSSDPFGAPAPAAAPMNSDPFGAPPAAPAAPMNSDPFGAPPAAP
                     PAAPSGGAMNSDPFGAAPPPSN"
     gene            3303..4088
                     /locus_tag="PLP58_20560"
     CDS             3303..4088
                     /locus_tag="PLP58_20560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ10985.1"
                     /translation="MTKVLFILSAIVTLVAAVFAYQNGREFSSIRQAVIAMNKDVDAQ
                     LAAATAVVAQVTKLNGDIATVQQELDVEGEKIKAQKLKIAQLDNDSKRVQDELDAKNK
                     KLAELNVLLGKLPVGVKPETLVEDINNMKKAIAEAEAEAEMKKKEVAAEEAKVADLQR
                     ALDDVIRKIEDRKKSFDRNSLNAQIVAVNSDWGFVVVNAGQSLGITEATKLLVTRGTQ
                     TIGKLSIVSVQGDRTVANILSDTLAKGEQISPGDRVILENLYQ"
     gene            complement(4228..>5129)
                     /locus_tag="PLP58_20565"
     CDS             complement(4228..>5129)
                     /locus_tag="PLP58_20565"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HRJ10986.1"
                     /translation="RGRILKDPLFGHYWREVAEGAWMGSRLRSALMALLLKALHRSGW
                     LEAVHTWIAISDFMRRRLIEAGAVHPARIVTLRHAWQAMPAAPSAEDAGHYLFLGRLV
                     HEKGVATLLDAWDTVHQRLGRTTPRLHIAGEGPLSGLVSERARRNPFVCALGPIGGQS
                     KEDQLRQARAVIVPSVWWEPLGLVTYEAYDHAKPVLAARSGGLAETVEHGQTGLLHEP
                     GNAGELAGQIFEMEAMSATSRAALGHAGRRWLLTEANPARWLRQFDEILTGCLESAAL
                     RHHRTVAGVALPFRRTIPGQGAV"
BASE COUNT         1003 a         1712 c         1547 g          867 t
ORIGIN      
        1 gagcaagccg ccgcgcgcga ggtgcgcgag gagaccggcc tccatcttcc cgccgttgcc
       61 ttccgccctc tggccactct gcccaacgag tacgagttcc aggattatct ctggcccacg
      121 ctcgacctct tttacaccgt ccaggccccg gaaaacgccg ccctgcggcc cgaccctgcc
      181 gaagtctccg aagccctctg gctgcccccg gaggatgccg ccatgtgcga cttcgcgttc
      241 gaatccaacc gccgcgccgt gcgcctgctg ctctctgccc ggaatcaggg gccgcaaacc
      301 caaggcgaag cacataactt gcctcatttc tgtcaaaatg ccgcctgaca ccgtttcccc
      361 catttacccc gcaccttcga tgctgtgact atgcaggaac aacttcgcag cgttgcatcg
      421 cgattgtcgg gcgttcagac aagcccgtgg agccgcggtt caaagcgatt atgcagcctt
      481 gcaaaacaac tgtgcaagaa gggctggggg aatcgcgccc acgccgctcc tcacttcatg
      541 tgcgaccagg cgatctcgcc cacgaggtcg ccgtttttgt ctatctgtac ctgggccagc
      601 cacggctcga agccgagcat catggcgcgg gagacgatgc tgtcgtggtc gtgctgggag
      661 atttccggcg gatgacccgg cgcgattgcc gtgttcacat ggacgaggag ctggctctcc
      721 ccgccgcgcg cctcgaagtc cctgacgccc tggcgctggt tgtggtggca gtggtagccc
      781 tgggcggcga gccatttctc cgcgtgtcgt tcaccgtatt ccgcgatctc gttgggtctc
      841 aacatggccg tgcagtttgg gttggcataa accgcccgcg caggcaggag cgccgtcgcc
      901 cggcggttta tttattcggt tataccggtc cggcggaaaa acggcaatcc ttcaatgcaa
      961 aaaaatcttc cgcgcccgcc ggcctcaggc gtgcatcacc tggccgccat ccacgttcag
     1021 cgtctggccg gtgatgttcc tcgcgcacgg ggaggcgagg aagacggcca tcgcggccag
     1081 ttcctccggc gtctgccagc ggcccagagg ggagaccttg cgcatttttt cctccgccca
     1141 cgaatcgtac ccctgcctct cagcctccgg gagccgcgcc tgcgccgccg cccagaccgc
     1201 ctcgttcaag ggcgtgcgca ccatgccggg agacagcgcg ttcacgcgca cgtcgcgcgg
     1261 cgccaggtcc ttcgccgcac actgcatgaa attgatcagc ccggccttcg ccgcgctgta
     1321 gggcgggtcg gtctgggagc cggtctgccc ggccacggag accaaaaaga gcagtgtgcc
     1381 gccgccgcgc gcgatgagcg cgggggcgaa agcgtgcgcc gtgttcaccg cgccgaccag
     1441 gttgatctcc agcacgcgct gccagtcgcc cggctccaga ttccaaaacg gaaaaccaaa
     1501 cttgcccgag cccgcgcccg cagcgaacac gacatgatcc accgcgccgc agtccgccgc
     1561 aaaggcgcgc agggccgagt aatccgtaac atcccccgtg gtgatggcga ctccctccga
     1621 cgcgcgcaga tcaaagccgc gcaccgcgca tccctccgcc tcaaaacccc gcgcgatggc
     1681 gcgccctatg ccgctggcgg cgccgatgac cacggccgtc tgtcctttga gttgcaaatc
     1741 catccgccac cctggcaccg cgcgcatgag caggcaagcc gccgcgggga ggattcatcc
     1801 gggttgtgcg ggatgagaaa atggtcgggc cggcgagatt cgaactcacg acctcttgca
     1861 ccccatgcaa gcgttctacc aggctgaact acggcccgac ctttttgcgg ggcgtgggtc
     1921 acgcccggcg gggacggcga tattggcgcg gcgtgccgga cagtgcaacc tgcatgttga
     1981 atttttggcg aaatttggcc ccgtcatcct gccgcgggct tgcaaatcaa gagaccgggg
     2041 agcttggatg cggaaggaat ttcgagtcct cccacaaaga cagccccctg attttcggga
     2101 cgtaaaatga aaagtctgaa aaatgctgtt gtcgaaaaag ccaggattcg gtagaaagcg
     2161 gctgttaatt cacatatccc gacaaaatca tggcctggaa actactctcc attctctccg
     2221 ccgtctgcct tggtgccgcg tgctacttcg ctcacgccaa caaacagcgc ctggaaaacg
     2281 aaaaaacccg ccaggctgcc gccgaggcca atctcaaggc cgcccaggat cgcaaagccg
     2341 agggcgatgc cgcggtcaag gtgaaggagg agcaatacgc ggccatccaa aaggagcgtg
     2401 acgccgccaa ggaggaagtg gtgaagctgg ccgccgacgc gcaggaaaaa gaggcagcct
     2461 tgtccgtcgt aaagggcaat ctggagcagg tgaccaccca ggtgaacgcc gtgcagaagc
     2521 agattgatga cgcgggtgac atcgaaaaac tcatcgccca ggtcgaaaag ctcaaaaagg
     2581 accagaccga agccgaggga gccgttgcca accaaaaaca gcggcttgcc aacgtccagg
     2641 ccaccatcgc cggcgtcacc gggcagattg acaagctcaa ggaaaccgag gcccagggcc
     2701 gccggggcat cgtggcgccg gagttctccg cgcgggtcgc ccaatttttc cccgaatggg
     2761 gtttcgccat cctgaacaag ggcaactccc agggagtttt cgccaacgcc gaccttgagg
     2821 tcaagcgcgg caaggatgtc atcgccagac tgaaagtgcg cagcgtcgag cagcacaatg
     2881 ccatcgctga cgtggtcccc ggctctgtcg ctgagggcag cagcattttc aacggcgact
     2941 ctgtcgtggc tgcggcgcag cagagcgcgg cagacccgaa aaaagggtct tcgtccacag
     3001 gaggcacgcc tgagcagggc gctcccgtgc cagacagtac cgctccccag gctcccgcca
     3061 tgtcctccga tcctttcggc gctccggcac cggcagccgc gcccatgaat tccgatccct
     3121 ttggcgcgcc tccggccgct ccagccgcgc cgatgaactc cgatcctttc ggcgccccgc
     3181 cggccgctcc tcctgcggcg ccatcgggtg gcgccatgaa ctccgatccc ttcggagcag
     3241 cacctcctcc ctcaaactga tccactttcc gctgaaccca tagaacttca ttttcatccg
     3301 ccatgaccaa agtccttttc atcctcagcg ccatcgtcac cctggtggcg gcagtgttcg
     3361 cttatcaaaa cgggcgtgaa ttcagcagca tccgccaggc agtcattgcc atgaacaaag
     3421 atgtggacgc gcagctcgcc gcggccaccg ccgtcgtcgc ccaggtgacg aagctcaatg
     3481 gcgacatcgc cacagtgcag caggaactcg atgtcgaggg cgagaaaatc aaagctcaaa
     3541 agctcaagat cgcccagttg gacaatgact ccaagcgcgt gcaggacgaa ctggacgcca
     3601 aaaacaagaa gctggccgag ttgaatgtgc tgctcggcaa gctgcctgtg ggagtcaagc
     3661 ccgaaacgct ggttgaggac atcaacaata tgaagaaggc cattgccgag gccgaggccg
     3721 aggccgagat gaagaagaag gaggtggcag ccgaggaggc caaggtggcg gacctccagc
     3781 gcgctctgga tgacgtgatc cgcaagattg aggaccgcaa aaaatccttc gaccgcaaca
     3841 gcctgaacgc gcagatcgtg gccgtgaact ccgactgggg attcgtggtg gtgaacgccg
     3901 gccagtctct cggcatcacc gaggccacca agctgctcgt cacgcgcggc acccagacca
     3961 tcggcaagct cagcatcgtt tccgtgcagg gagaccgcac cgtggcgaac atcctttccg
     4021 acaccctggc caagggggag cagatttctc ccggagaccg tgtcattttg gaaaatctct
     4081 atcagtgaga cctgccacgg cgcatctgtg cgagtcatga acccacgccg cattcttcat
     4141 tccctgatct tccttgccgc cgcccagttt cttgtgtcct gcgcgtcgga tgaaaccaag
     4201 cgccgcgagc gtgtgccgcc gactgtgtca gacagcaccc tgccctggaa tcgtccgccg
     4261 aaatgggagg gcaacgcccg ctacggttcg atgatgccgc agagccgctg attccaggca
     4321 gccggtcaat atctcatcaa actggcgcag ccagcgcgca gggtttgcct ccgtcagcag
     4381 ccagcgcctg cccgcgtggc ccagcgcggc ccgcgaagtg gcggacatgg cttccatctc
     4441 aaaaatctgg ccggccagct ccccggcatt tccgggttcg tgcagcaggc ccgtctgtcc
     4501 atgctccacg gtttcggcca gtccgcccga gcgcgccgcc agcacgggtt tggcgtggtc
     4561 gtaagcctcg taggtgacca ggccgagagg ttcccaccag accgagggca cgatgaccgc
     4621 gcgcgcctgg cggagctggt cctccttgct ctggcctccg atggggccga gcgcgcagac
     4681 aaatggattg cgccgcgcgc gttcgctgac cagtccggat agcggcccct ccccggcgat
     4741 gtgaagcctc ggcgtcgtcc tgccgagccg ctgatggacc gtgtcccagg cgtccaggag
     4801 cgtggcgaca cctttttcat gcaccaagcg gcccaggaaa agataatggc cggcgtcttc
     4861 cgcggacggc gcggctggca tggcctgcca ggcatggcgc agagtgacga tgcgcgcggg
     4921 atgcaccgcc ccggcttcga tcagccgccg ccgcatgaag tccgagatgg cgatccatgt
     4981 gtggaccgct tccagccagc cggagcggtg cagtgctttg agcaggagcg ccatgagcgc
     5041 gctgcgcagg cgcgagccca tccaagctcc ctcggctacc tccctccaat aatggccgaa
     5101 gagcgggtcc ttgaggatgc gtccgcggg
//
LOCUS       DAOMYJ010000305         5064 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Prosthecobacter sp. isolate AMR_MDS_5629
            NODE_5712_length_5064_cov_19.426233, whole genome shotgun sequence.
ACCESSION   DAOMYJ010000305 DAOMYJ010000000
VERSION     DAOMYJ010000305.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867850
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prosthecobacter sp. (wastewater metagenome)
  ORGANISM  Prosthecobacter sp.
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Verrucomicrobiaceae; Prosthecobacter.
REFERENCE   1  (bases 1 to 5064)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5064)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.30
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:34:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,742
            CDSs (total)                      :: 4,684
            Genes (coding)                    :: 4,676
            CDSs (with protein)               :: 4,676
            Genes (RNA)                       :: 58
            tRNAs                             :: 55
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5064
                     /organism="Prosthecobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5712_length_5064_cov_19.426233"
                     /isolate="AMR_MDS_5629"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1965333"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            248..322
                     /locus_tag="PLP58_20570"
     tRNA            248..322
                     /locus_tag="PLP58_20570"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:280..282,aa:His,seq:gtg)
     gene            376..1113
                     /gene="rnc"
                     /locus_tag="PLP58_20575"
     CDS             376..1113
                     /gene="rnc"
                     /locus_tag="PLP58_20575"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR02191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004525 - ribonuclease III activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HRJ10987.1"
                     /translation="MSPRLEAAQNIAGHVFQNPALLEQALTHSSVNHNSAKERAPNNE
                     RLEFLGDAVLQLALSEWLYERLQTADEGALTKLRAQLVSTRALARLSRQLGLGSHMLI
                     GRGEEAVGGRENENILADVVEAVLGAIYLDGGLAAAQGFVRRHFAEALDAAGRLEHEG
                     NPKGCLQEICQTLGPVNLRYEIIAESGLDHAKNFTAAVLWDDEPLGTGTGASKKEAEI
                     RAASAALESEALQSSLKRRGAPIIQPP"
     gene            1408..2484
                     /gene="aroC"
                     /locus_tag="PLP58_20580"
     CDS             1408..2484
                     /gene="aroC"
                     /locus_tag="PLP58_20580"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018290835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004107 - chorismate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HRJ10988.1"
                     /translation="MSSSFGTLFRISTWGESHGPGVGVVVDGCPPRIPLTEADLQHEL
                     DRRRPGQSKIVTPRKEDDKAEILSGVLDGQTLGTPIGILVRNTDQRPSAYTEMQQAYR
                     PSHADYTYDAKYGIRAVSGGGRSSARETIGRVAGGAIARKVLQHSLPGYECLAYVKTV
                     RDLTAAVPARLSAEVIESNIVRTCDPAAAEQMIVLIEAMRSEGNSVGGVIECVVRGVP
                     AGLGAPVFDKLEADLAKAMMSLPATKGFEIGSGFAGTQMTGLEHNDEFYMDEGRVRTR
                     TNRSGGIQGGISNGEDIVFRVAFKPTATVLREQKTVTNTGEDTTLSARGRHDPCVLPR
                     AVPMIEAMTHLVLADHLLRQRALG"
     gene            2650..3987
                     /locus_tag="PLP58_20585"
     CDS             2650..3987
                     /locus_tag="PLP58_20585"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HRJ10989.1"
                     /translation="MAGGGGCGIVSGHGEVLNPHVVDNQDHFHPATMIPTRARFIVLA
                     GLCSAAALAYLVRNGVAPAESTIRADLGLTKEQSGLMMSAFFWPYALCQIPAAMLAQR
                     LGSRRALALYAVMWSLATAGFALGELGWMTASRAFMGVAQAGLVPVAVGVMARWFPKT
                     AQATAAGAFSGFMSAGSIVAAPLTAWLVMDLGWRWMFVLYAIPGVLWAAWFAWWHRDD
                     PARHPAVNEAELERIGPAGTKAGDDAATGVWRALAVNPALWLLCVQQFCRAAGYIFFA
                     SWFATYLQEARGITLVKSAWLTTLPLLADVAGGFSGGLISDALLRRTRSRRIARQGMA
                     MAALLLCAALIFTAWFVADPVLAVLVISAGMYCAGVVNPCFSATLMTCGGPHAATWSA
                     VTNMCGNFGAASFPILVPWLIAHAGGWDAVLAGFGSIYILAALLWALLRGGRE"
     gene            4074..4709
                     /locus_tag="PLP58_20590"
     CDS             4074..4709
                     /locus_tag="PLP58_20590"
                     /inference="COORDINATES: protein motif:HMM:NF018178.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1080 domain-containing protein"
                     /protein_id="HRJ10990.1"
                     /translation="MKHLHFLSPLLCALTLPVLADEGFTPLFDGKTMNGWRNAYEWGD
                     IEVVNGEIHLTGDKKFFVVTEKTYSDFIFEGEILLPEGQANSGFMFRAHVEPNKVFGY
                     QAEVDGDANRKWSGGLYDEGRRKWFISPVKGDKESEAAFRARAGDCFKRNDWNTYRIT
                     CQGKKLKIEVNGVVTTDVVDDMDASGVIAIQHHGEKGQTYKFRNLRIKELK"
     gene            4743..>5064
                     /locus_tag="PLP58_20595"
     CDS             4743..>5064
                     /locus_tag="PLP58_20595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009960823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent transferase"
                     /protein_id="HRJ10991.1"
                     /translation="MKDDPTVPPDARFETRAIHLGQDYRAETGAVIPPIYMTSTFETG
                     NPGGFDYTRSGSPNFRNLQTTLASLENARHCTVFASGVSAITAVVFGLKAGDVILSEQ
                     NIYGC"
BASE COUNT          895 a         1662 c         1620 g          887 t
ORIGIN      
        1 cgggtccgcc gggacgtgcc ggcagggagg catggcgagg aaacacgggc cgcctaccct
       61 tgcgcaagtc gcagggaaag aaggagggcg gcgctgttgc ggcgggtcac ggcgtagtgg
      121 tagacccgcc gccgcgatgt tgcacggact tttttcctcc ggcccggcgc accgttcccg
      181 cgccatgaaa aaaaccgcta gcacatgcag ggaggggcgt tatcatcgtg ccgcttccag
      241 cgaaatggtg gtcgtagctc aatggtagag ccccagattg tgattctggt tgttgcgggt
      301 tcgagccccg tcgatcaccc cacgcttccc cgccattcag attgatggcg gcattttttc
      361 ggatacttcc gagtcatgtc tccccgcctc gaagccgcgc aaaacatcgc cggtcatgtg
      421 tttcaaaatc ccgcgctgct tgagcaggcc ctcacgcaca gcagcgtaaa tcacaactcc
      481 gccaaagagc gcgcgcccaa caatgagcgg ctggaatttc tcggcgacgc ggtgctgcag
      541 ctcgcgcttt ccgaatggct gtacgaacgg ctgcaaaccg ccgacgaggg cgcgctgacc
      601 aaactgcgcg cgcagctcgt ctcgacccgc gccctggcgc gtctctcacg gcagttgggc
      661 ctcggcagcc acatgttgat cggccgcggc gaggaagcgg tgggcggccg cgaaaatgaa
      721 aacatcctcg ctgatgttgt cgaggccgtc ctcggagcca tctatctcga tggcggactc
      781 gctgcggcgc agggctttgt gcgccggcac ttcgcggagg cgctggatgc cgccgggcgt
      841 ctcgagcacg agggcaatcc caaaggctgc ctccaggaaa tctgccaaac ccttggaccc
      901 gttaacctgc gctatgaaat catcgccgaa agcggcctcg accacgccaa gaatttcacc
      961 gccgccgttc tctgggacga cgaaccgctc ggcaccggca ccggggccag caaaaaggag
     1021 gccgagatcc gcgccgcgag cgccgcgctg gaaagcgagg cactccagag cagcctgaag
     1081 cgacgaggcg cgccgattat acagccacca taattcccgg aacaacatgt gcgcaacact
     1141 gcgaacaaac ccctcaatgc tgagagtcac gcgcacaact gcgacttgtt cctgagcgcg
     1201 cacagactgt gcgcattttt gttcgccgat catgcgggcg tggaacagcg gcggcacaaa
     1261 catggaaccc gcttgttgca gttcctcaca ccgcctacgg ctgggaagaa tttgaatttt
     1321 tcacctccaa tcattccaga gctgcgcaca actccgaaaa cttctccctg cttgcagtca
     1381 caccgtgacc gcccatcatg aacgaacatg tccagcagct ttggcacgct ttttcgaatc
     1441 agcacttggg gggaatccca cggtcccggc gtgggcgtgg tcgtggatgg ctgcccgccg
     1501 cgcatccccc tcaccgaggc cgacctccag catgagttgg accgccgccg tcccggccag
     1561 agcaagatcg tcaccccgcg caaggaggac gacaaagccg agatcctctc cggcgtgctc
     1621 gatgggcaga ccctcggcac ccccatcggc atcctcgtgc gcaacaccga ccagcgcccc
     1681 tccgcctaca cggagatgca gcaggcctac cgccccagcc acgccgacta cacttatgac
     1741 gccaagtacg gcatccgcgc cgtctccggc ggcggccgct ccagcgcccg cgagaccatc
     1801 ggccgcgtcg ccggcggagc catcgcgcgc aaggtgctcc agcattccct gcccggctac
     1861 gaatgccttg cctatgtgaa gaccgtgcgt gacctcacag ccgccgtccc cgcccggctc
     1921 agcgccgaag tcatcgagtc gaacatcgtc cgcacctgcg accctgcggc ggcggagcaa
     1981 atgatcgtcc tcatcgaggc catgcgcagc gagggcaaca gcgtcggcgg cgtcatcgag
     2041 tgcgtcgtgc gcggcgtgcc cgccggactc ggcgcgcctg tctttgacaa gctggaggcc
     2101 gacctcgcca aagccatgat gagcctgccc gccaccaagg gtttcgagat cggcagcggc
     2161 tttgccggca cgcaaatgac cggcctggag cacaacgacg aattctacat ggacgagggc
     2221 cgcgtccgca cccgcaccaa ccgcagcggc ggcatccagg gcggcatcag caacggcgag
     2281 gacatcgtct tccgtgtcgc cttcaagccc accgccaccg tcctgcgcga gcagaaaact
     2341 gtcaccaaca ccggcgagga caccaccctc tccgcccgcg gccgccatga cccctgcgtg
     2401 ctcccccgcg ccgtgcccat gatcgaggcc atgacccatc tcgtgctcgc cgaccacctc
     2461 ctgcgccagc gcgctctcgg gtagggcggc gggaaaggcg gggcgtgttg tcctcaacgc
     2521 gcagcgtgct cactgcacac ggagtttgga cgatggaccc acgctcgcgg cggcttgagg
     2581 acaagccgga ctggttgtgg ggactctcga aaactcagag catgggctgc gggcatcctg
     2641 cttgcgcgaa tggcgggcgg cggtggatgc gggattgttt ccgggcatgg tgaagttcta
     2701 aacccgcatg tcgttgacaa tcaggaccac ttccatcctg ccaccatgat acccacccga
     2761 gcccgtttca tcgtcctggc cgggctgtgc tcggcggcgg cgctggcgta tttggtgcgg
     2821 aacggcgtgg ctccggcgga gagcacgatt cgtgcggacc tgggactcac gaaagagcag
     2881 tcgggcttga tgatgagcgc gtttttctgg ccgtatgcat tgtgccagat cccggcggcg
     2941 atgctggcgc agcggctcgg gtcgcgtcgg gcgctggcgc tctatgcggt gatgtggtcg
     3001 ctggccacgg caggctttgc gctgggcgaa ctcggctgga tgacggcctc gcgggccttc
     3061 atgggtgtgg cgcaggcggg actggtgccg gtggcggtgg gcgtgatggc gcgctggttt
     3121 cccaaaacgg cgcaggctac cgctgcgggc gcgttcagcg ggttcatgtc cgcgggcagc
     3181 attgtggcgg ccccgctgac ggcgtggctg gtgatggacc tgggctggcg ctggatgttt
     3241 gtgctgtatg cgattcccgg tgtgctgtgg gcggcgtggt ttgcctggtg gcatcgtgac
     3301 gacccggcgc ggcatccggc ggtgaatgag gcggagttgg agcgcatcgg gccagccggc
     3361 acgaaggccg gggatgatgc cgcaaccgga gtctggcgtg ccttggccgt caatccggcg
     3421 ctttggctgc tgtgcgtgca gcaattctgc cgcgcggcgg gctacatctt cttcgcgagc
     3481 tggtttgcga cctatctgca ggaggcgcgg ggcatcacgc tggtgaaatc cgcgtggctg
     3541 accacgctgc cgctgctggc ggatgtggcc ggcggttttt ccggcgggct gatctcggac
     3601 gcgctgctgc gccgcacgcg cagccgccgc atcgcgcgcc agggcatggc gatggcggcg
     3661 ctgctgctct gcgcggcgct catttttacg gcgtggttcg tcgccgatcc ggtgctggcc
     3721 gtgctcgtga tcagcgcggg catgtattgc gccggggtcg tgaatccgtg cttctccgcc
     3781 acgctgatga cctgcggcgg acctcacgcg gcgacatgga gcgccgttac gaacatgtgc
     3841 gggaatttcg gcgcggcatc gttcccgatc ctcgtgccct ggctgatcgc gcatgcgggc
     3901 ggctgggacg cggtgctggc gggatttggc tccatttaca tcctggcggc gctgctgtgg
     3961 gcgctgctgc ggggcgggcg ggagtgaggc gcggcgcggg gagccctcgg ccacgcgcca
     4021 gacaaaggcg cttttcatcc gcccgctttt cggcatgatc accgcctcga accatgaaac
     4081 accttcattt tctctccccc cttctctgcg ccctgaccct gcccgtgctg gccgacgaag
     4141 gtttcacgcc gctctttgac ggcaaaacga tgaacggctg gcgcaacgcc tacgagtggg
     4201 gcgacatcga agtggtgaat ggggagatcc atctcacggg cgacaaaaaa ttcttcgtcg
     4261 tcaccgagaa gacctacagc gacttcatct tcgagggcga aatcctcctg cccgagggcc
     4321 aggccaacag cggcttcatg ttccgcgccc atgtcgagcc caacaaagtc ttcggctacc
     4381 aggccgaggt ggatggcgac gccaaccgca agtggtccgg cggcctctac gatgaaggcc
     4441 ggcgcaagtg gttcatcagc cccgtcaagg gtgacaagga aagcgaggcc gccttccgcg
     4501 cgcgcgcggg cgactgcttc aagcgcaatg actggaacac ctaccgcatc acctgccagg
     4561 gcaaaaaact caagatcgag gtcaatggcg tggtcaccac cgatgtcgtg gatgacatgg
     4621 atgccagcgg tgtgatcgcc atccagcacc acggagaaaa aggccagacc tacaagttcc
     4681 gcaacctgcg catcaaggaa ctgaagtgag gggatccgat ttcgagcttc gatttttccc
     4741 ccatgaaaga cgaccccacc gtgccgccgg acgcgcgttt cgaaacgcgc gccattcatc
     4801 tcggccagga ttaccgcgcc gagacaggag cggtgatccc gcccatctac atgacctcga
     4861 ccttcgagac ggggaacccg ggcggctttg actacacacg ctctggcagt ccgaacttcc
     4921 gcaacctgca aacgacgctc gcctcgctgg aaaacgcccg gcattgcacc gtcttcgcca
     4981 gcggcgtctc cgccatcacg gcggtggtct ttggcttgaa ggccggggat gtgatcctct
     5041 ccgagcagaa catttacggc tgca
//
LOCUS       DAOMYJ010000306         5036 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Prosthecobacter sp. isolate AMR_MDS_5629
            NODE_5752_length_5036_cov_23.726962, whole genome shotgun sequence.
ACCESSION   DAOMYJ010000306 DAOMYJ010000000
VERSION     DAOMYJ010000306.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867850
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prosthecobacter sp. (wastewater metagenome)
  ORGANISM  Prosthecobacter sp.
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Verrucomicrobiaceae; Prosthecobacter.
REFERENCE   1  (bases 1 to 5036)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5036)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.30
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:34:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,742
            CDSs (total)                      :: 4,684
            Genes (coding)                    :: 4,676
            CDSs (with protein)               :: 4,676
            Genes (RNA)                       :: 58
            tRNAs                             :: 55
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5036
                     /organism="Prosthecobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5752_length_5036_cov_23.726962"
                     /isolate="AMR_MDS_5629"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1965333"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            234..848
                     /gene="ruvA"
                     /locus_tag="PLP58_20600"
     CDS             234..848
                     /gene="ruvA"
                     /locus_tag="PLP58_20600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009965909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009379 - Holliday junction helicase
                     complex [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HRJ10992.1"
                     /translation="MIAFLRGKITSALPHQAVLDVAGVGYAVNIPLSTFDRLPQSGTE
                     ATLLTHHHVTEHEHLLFGFHTEEERDLFRLLIDRVSGIGPKMALSVLSGMPVPAFKDA
                     VIRGDTAALARIKGVGKKTAERIVLELKDKVGVVTAWQAAEAARGAHDPRQEAASDAV
                     LGLIALGFKQAEAQKAVTELLKQPAFDTAPQADKLIREALRLLQ"
     gene            891..3284
                     /locus_tag="PLP58_20605"
     CDS             891..3284
                     /locus_tag="PLP58_20605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ10993.1"
                     /translation="MHRRPRHHRPQAPKTSKAPPAKPLASLLDECPELRAADRGPLPR
                     EFWKALDEKHAPRPDYLLWRVVLSGGPAPAGLREDAAALALRALTSPDPCVALGALVL
                     LPGLKIEQVPPQRLAVLPAALMKMRVPSGQRLAHWLAAGKAGGTTAPEGALRLAELEE
                     ILRTDPSWKPGRAPDPGFLASAMSPVPAAKAARWLAAQIHSETRRLRQNAETLAAQAD
                     WHLTTWLLLRDSRPAHAPALPAEVEALLNAASASRLNNRPLEAARWTALALHLLPPRV
                     PEILLQRARVAAWSLAEAGIEAPAGLGVSLDDLPFPGENPGTPKGREVAALFHDPEDA
                     SLACLARAIANDPDWSSLAAAGVELHHPLAALAWVARKAQSHHLKKQHALLDAAARLA
                     ARHHCLGSQGKILAQWPGGATAVLDYARALRLSLRQMPYLRDAAAWERMTSCLRAAWG
                     RLEPEAITDPEDVFFIHETLLDREATTLRRLPPALRGNALRHPHSRRTPSALVRELAS
                     EPKLLRQLEHQRRLELWEIAAALRERAGLANTVWVSLASVGEPGCGRFSLLVLGREGR
                     RHIMGRLRSGTDGLLDMAPLAAEIARSIRIAAPDASWVLLAADSGWQRIPWAGLFHQE
                     GLDVMIALLPGWEWAFRVMREPGLEQPPPARVLLPENMPPPQAVPALPAGKDSSHACL
                     LLSGAEMCDASTRWHPLPAQEQEKPLYSLSLGSHPLVISMSVLKPGSLRDDLVRQCLA
                     QSTRRVLAPARPLTKDEAALYETHAASVDPSAPLESRLLRALPDAWLLHGLPSLCEH"
     gene            3437..4369
                     /locus_tag="PLP58_20610"
     CDS             3437..4369
                     /locus_tag="PLP58_20610"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009958572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004749 - ribose phosphate diphosphokinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0006015 - 5-phosphoribose 1-diphosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009156 - ribonucleoside monophosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009165 - nucleotide biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate pyrophosphokinase"
                     /protein_id="HRJ10994.1"
                     /translation="MNEQLKILSGSAHPELAKLICQHLGANLCDATLTTFPDGETFVQ
                     IKENIRGGDIFIVQPTCPPTNQNLMELLIMVDAVRRASAHRITAVLPFFGYARQDRKD
                     RPRVPITAKLVANLLVASGVHRVLTVDLHAGQIQGFFDIPVDHLYAAPVLMKAIRERG
                     IGDLVVVSPDVGGIKMTHAYAKALNAPMAIVAKNRVSAEEVEALNVIGDVAGKNVLLV
                     DDLTETAGTLTAAADLLLKHGAGKIYAGVSHAVLGEKGKARIAESPIIELFATNSVPQ
                     AAGEKVTTLDISGLLAQAILRIHNNESVTSLFDI"
     gene            4432..>5036
                     /locus_tag="PLP58_20615"
     CDS             4432..>5036
                     /locus_tag="PLP58_20615"
                     /inference="COORDINATES: protein motif:HMM:TIGR00731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0008097 - 5S rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25"
                     /protein_id="HRJ10995.1"
                     /translation="MAKILDIQAEPRASVGTTAVNRLRKSGVIPGVIYGRKTAPSNVQ
                     VDRKTFTKLLESSASDNILVSLKMGADSQLALVQEVQHDHLRGGILHVDFHAVAMDEE
                     IHAQVPVVLVGESPGAKLGGLVEIFHHNIEVRCLPKDLPESIDIDVTSLDLGNSVNVG
                     DLKLPEGVRAVLAADVLVLHCEAPKVAEEAPAAAAAPAAAAP"
BASE COUNT          866 a         1778 c         1532 g          860 t
ORIGIN      
        1 gtagtcgcgg cggaggcggc ggctggagcc ggggctgggg aaggcggcga aacgggcgct
       61 ggagtctcct gcgccggaag cccggcggcc agcagcagga ggatgtaaag acggtatgtc
      121 atgccaggag cagttcctcg tggcttgtgt gcgcgcgcgc tgctcgtcat ttcgcggacc
      181 agcctgcgca tcaaacctct tgcacttaac atgcctcccg gcttcatcaa ggcatgatcg
      241 cttttctgcg cggaaaaatc acctccgccc tgcctcacca ggccgtgctc gatgtcgccg
      301 gcgtcggtta tgccgtgaac atcccgcttt ccaccttcga cagactgccg caatccggca
      361 cggaggcgac cctgctcact catcatcatg tgacggagca cgagcatctg ctgtttggct
      421 tccacaccga ggaggagcgc gatttattcc gcctgctcat tgaccgcgtc tccggcatcg
      481 ggccgaagat ggcgctctcg gtgctcagcg gcatgcccgt gccagctttc aaagacgccg
      541 tcatccgcgg cgacacagcc gctcttgcgc gcatcaaagg tgtggggaaa aaaaccgccg
      601 agcgcatcgt gctcgagttg aaggacaagg tcggcgtcgt caccgcctgg caggccgccg
      661 aagccgcgcg cggcgctcat gatcctcgcc aggaggccgc cagtgacgcg gtgctcggtc
      721 tcatcgcgct gggcttcaag caggccgagg cgcaaaaggc cgtcaccgag ctgctcaagc
      781 agccagcctt tgacaccgcc ccgcaggctg acaagctcat ccgcgaggcg ctgcggctcc
      841 tgcaataaat agtcccgcag ccaggcactc cgccccattg agagacaccc atgcaccgac
      901 gcccgcgcca tcaccgcccg caagcgccca agacttcgaa ggctcccccc gcaaagccgc
      961 ttgcctcgct tctggacgag tgccctgaac tgcgcgcggc agacagggga cctctgccgc
     1021 gcgagttttg gaaagccctg gatgaaaaac acgcgcctcg gcctgactac cttctctggc
     1081 gtgtggtgct ctcaggcggt cccgcgcccg ccggcctgag ggaggacgcc gccgcgctgg
     1141 cgctgcgcgc tttaacttcg cccgatccct gcgttgcctt gggggcgctc gtcttgctgc
     1201 cgggactgaa aatcgagcaa gtgccgccgc aaagactggc cgtgctgccc gccgcattga
     1261 tgaaaatgcg cgtcccgtcc ggacaaaggc tggcgcattg gctggcggcg gggaaggcag
     1321 gcgggacaac cgcacccgag ggggcgctgc gtctggctga gttggaggag atcttgcgaa
     1381 ccgacccttc ctggaagcct ggccgcgcgc ctgatcctgg attcctcgcc tccgcgatga
     1441 gtcccgtgcc cgccgccaag gccgcgcgct ggctggcggc gcagattcac agcgaaaccc
     1501 gccgactccg gcaaaatgcg gagacccttg cggcacaggc agactggcat ctcaccacct
     1561 ggctgctgct gcgcgactcg cgcccggccc atgcgcccgc gctgcctgca gaggtggagg
     1621 cgctgctcaa tgccgcctcc gcgtcccgtc tgaacaaccg gcctttggag gccgcgcgct
     1681 ggacggctct ggcgctccat ctgctcccgc cacgcgttcc ggaaatactc ctccagcgcg
     1741 ctcgtgtggc cgcctggagc ctggccgagg ccggcatcga ggcgcctgcc ggtctcggtg
     1801 tgtctttgga cgacctgcct ttccccggtg aaaaccccgg cactcccaaa ggcagggagg
     1861 tggcggccct tttccacgat ccggaggatg cctcccttgc ttgtctggcg cgcgcgatcg
     1921 cgaatgatcc cgactggtca tcgctcgcgg cggcaggagt ggaactgcat catccgctgg
     1981 ccgcgctggc ctgggtggcc agaaaagccc aatcgcatca tttgaaaaaa cagcacgccc
     2041 tgctggatgc cgcagcccgt ctcgccgcgc gtcatcactg cctcggcagc cagggtaaaa
     2101 tcctcgccca atggccgggc ggcgccacgg cggtgctgga ctacgcgcga gccctgcgcc
     2161 tgagcctgcg gcagatgccc tacctccggg atgccgcggc ctgggagcgc atgacctcat
     2221 gcctgcgcgc cgcatggggc cgtcttgagc cggaggccat cacggatccg gaggatgtgt
     2281 ttttcattca cgaaaccctg ctcgaccgtg aggcaaccac gctgcgccgc ctcccacccg
     2341 ccttgcgcgg gaacgccttg cggcacccgc acagccgccg gacaccctcg gcactggtgc
     2401 gggaactcgc gtcggaaccg aaattattgc gccagttgga gcatcaacgc cgcttggagc
     2461 tttgggagat cgccgccgcg ttgcgcgagc gcgcgggtct cgctaacacc gtgtgggtga
     2521 gcctcgccag cgtcggcgaa cccggctgcg gccgcttcag cctgcttgtt ttaggccgcg
     2581 agggacgcag gcacatcatg ggccgccttc gctccggcac ggacggcctt ctggacatgg
     2641 cgccgctggc agcggaaatc gcgcgctcca tccgcatcgc cgcgccggac gcctcatggg
     2701 ttttgctggc cgccgacagc ggatggcagc gaattccatg ggcggggctt tttcaccagg
     2761 aaggtctgga tgtgatgatc gcgctgcttc ccggatggga atgggccttc cgcgtcatgc
     2821 gcgagcccgg gctggagcaa ccgccacccg cgcgtgtgct gctgccggaa aacatgccgc
     2881 ctccacaggc cgttcccgct ttgccagccg gcaaggattc gagccatgcc tgcctcctgc
     2941 tctccggcgc ggagatgtgc gatgcctcca cacgctggca cccactgcca gcgcaagagc
     3001 aggagaagcc tctctactcc ctcagcctgg gttcgcatcc gctggtcatc tccatgagcg
     3061 tgttgaagcc tggctcgctg cgggacgatc tcgtccgcca gtgcctggcg cagtccaccc
     3121 gccgcgtgct ggcacccgct cgaccgctga cgaaggacga ggcggcgctg tatgaaaccc
     3181 atgccgccag cgtcgatccc tcggcccctc tggaaagccg cctgcttcgc gccctgccgg
     3241 acgcctggct attgcacggc ctgccgtcac tctgcgagca ctgagagatg gatgactgcg
     3301 gacaaagcgg catggacgat gtgattcgga ctttgacaac caaaggcatc gcacaacccg
     3361 tccgggcggc tatctggtct ctgccacccg cctcttgatt tccgcgttga ttccgcctaa
     3421 cgagcaggcc gctcccatga acgaacaact gaagatcctc agcggatccg cccatcccga
     3481 actcgccaag ctcatctgcc agcacctggg cgccaatctc tgtgatgcca cgctgaccac
     3541 ttttccggac ggcgaaacct ttgtgcagat caaggagaac atccgcggcg gggacatctt
     3601 catcgtccag cccacctgcc cgccgacgaa ccagaacctc atggagctgc tcatcatggt
     3661 ggacgccgtg cgccgcgcca gcgcccaccg tatcaccgcc gtgctgccgt tttttggcta
     3721 cgcccgccag gaccgcaagg accgcccgcg cgtgcccatc accgccaagc tggtggcgaa
     3781 cctcctcgtc gccagcggcg tgcatcgcgt gctcaccgtg gacctgcacg ccgggcagat
     3841 tcagggcttt tttgacattc ccgtggacca cctctacgcc gctcccgtgc tgatgaaggc
     3901 catccgtgag cgcgggatcg gcgatctcgt cgtcgtctcg cccgatgtcg gcggcatcaa
     3961 gatgacccat gcctacgcca aagcgctgaa cgcccccatg gccatcgtcg ccaagaaccg
     4021 cgtcagcgcc gaggaggtgg aggctctcaa tgtcatcggc gacgtggcgg gaaaaaacgt
     4081 cctgctcgtg gacgacctca ccgagaccgc cggcaccctc accgctgccg ccgacctgct
     4141 gctcaagcat ggggccggca aaatctacgc cggcgtctcc cacgccgtgc ttggcgagaa
     4201 gggcaaggcg cgcatcgccg agtcgcccat catcgagctc ttcgccacga actccgtgcc
     4261 gcaggccgcg ggtgaaaaag tcaccacgct cgacatctcc ggactgctgg cccaggccat
     4321 tctgcgcatc cataacaacg agtctgtcac ttctctcttt gacatctagg aaagggcggc
     4381 tactctcccg gcccttttcc tcaaaacgac tgttcatagg agcaaacgac catggccaaa
     4441 atcctcgaca tccaagccga gccgcgcgcg agcgtcggca ccaccgccgt caaccggctg
     4501 cgcaaatccg gcgtcattcc cggcgtcatc tatggccgca aaacggcccc gtcgaatgtt
     4561 caggtggacc gcaagacatt caccaagctg ctggaaagca gcgcctcgga caacattctt
     4621 gtcagcctca aaatgggcgc ggattcccag ctcgccctcg tccaggaagt gcagcacgac
     4681 catctgcgcg gcggcattct ccatgtggac ttccacgccg tcgcaatgga cgaggaaatt
     4741 catgcgcagg tgcccgtcgt gctcgtcggt gaatcccccg gcgccaagct gggcggtctg
     4801 gtggaaatct tccaccacaa catcgaggtg cgctgcctgc ccaaagacct gcccgagtcc
     4861 attgacattg atgtcaccag ccttgacctc ggaaacagcg tcaacgttgg cgatctgaaa
     4921 ctgccggagg gcgtccgcgc cgtgctcgcc gccgacgtgc tcgtccttca ctgcgaggct
     4981 ccgaaagtgg ctgaggaagc cccggctgcc gcggccgcac ccgctgccgc cgcgcc
//
LOCUS       DAOMYJ010000307         5000 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Prosthecobacter sp. isolate AMR_MDS_5629
            NODE_5811_length_5000_cov_25.740546, whole genome shotgun sequence.
ACCESSION   DAOMYJ010000307 DAOMYJ010000000
VERSION     DAOMYJ010000307.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867850
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prosthecobacter sp. (wastewater metagenome)
  ORGANISM  Prosthecobacter sp.
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Verrucomicrobiaceae; Prosthecobacter.
REFERENCE   1  (bases 1 to 5000)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5000)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.30
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:34:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,742
            CDSs (total)                      :: 4,684
            Genes (coding)                    :: 4,676
            CDSs (with protein)               :: 4,676
            Genes (RNA)                       :: 58
            tRNAs                             :: 55
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5000
                     /organism="Prosthecobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5811_length_5000_cov_25.740546"
                     /isolate="AMR_MDS_5629"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1965333"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..212
                     /locus_tag="PLP58_20620"
     CDS             <1..212
                     /locus_tag="PLP58_20620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ10996.1"
                     /translation="KGSDARKVLLADLLWRRTVVSQEWLAERLRMSSAANVSQQLRRL
                     DRKRALGRVPEAMRNFLEQLDDSSP"
     gene            600..908
                     /locus_tag="PLP58_20625"
     CDS             600..908
                     /locus_tag="PLP58_20625"
                     /inference="COORDINATES: protein motif:HMM:TIGR02606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051983 - regulation of chromosome
                     segregation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system ParD family
                     antitoxin"
                     /protein_id="HRJ10997.1"
                     /translation="MPTQNVNLTPVLESFVKTQVSLGHFNNASEVHRSALAAMARQEE
                     ERALRLEQLRREIQLGIEDVEAGRWAKIGSVNGLGAMLDGCLEKAVERLELSNAEPAS
                     "
     gene            905..1255
                     /locus_tag="PLP58_20630"
     CDS             905..1255
                     /locus_tag="PLP58_20630"
                     /inference="COORDINATES: protein motif:HMM:NF016876.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HRJ10998.1"
                     /translation="MNLTRTAKAVEDIESCLLWSAENFGIPAARRYRALLEVALLAIL
                     EDPELCGSKLMEGFDGKVRIYHLRHSRKAAPVGGLIVKNPRHFIVYRITSDRAIELLR
                     VLHERMDFETQMNG"
     gene            1739..2164
                     /locus_tag="PLP58_20635"
     CDS             1739..2164
                     /locus_tag="PLP58_20635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ10999.1"
                     /translation="MNEESNAQEPEEEPSQAEVAKRLLTPEGRAFMAEIMRKTLAGDY
                     GELTEEVMQSAKAGLERVRSQEVMEEVMTRVEALRERLLAAQREKPECSMWAFMRDIN
                     EEMKELMDLALDVHEPRRSEIMPCLLSFQQHIEEFMKKL"
     gene            2177..2965
                     /locus_tag="PLP58_20640"
     CDS             2177..2965
                     /locus_tag="PLP58_20640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ11000.1"
                     /translation="MLSFRDEDWIFRHGPSRFEAWIMPGHRKPADVMWGLELHYKAGG
                     ARWRRVEEHVRPEVDLAIRSWKLKSGRWTDLTEVKYWNRNLEDQKRTGLPWFLTWEGG
                     MLDLCHRAEAGGKHEFSHGCEVFWQVREREGGRFLVELAAGVGREQPAEEKELVLPDG
                     TRETGERDPSDFWRETAAIYALEWVPFGKVTVRVPRNARDHLACARHRAHELLGISTP
                     PEAYHMQDFLVVREGREEEEAVAANSLHDDVFVKLHFHGYHEDE"
     gene            2967..3731
                     /locus_tag="PLP58_20645"
     CDS             2967..3731
                     /locus_tag="PLP58_20645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ11001.1"
                     /translation="MSWIQWRDETLPATQDEARMELRLHAPDMGGPRQCWSLRLAHEV
                     PWPDDGVKRKYRHQCWFTVELLEMGAQVRDVRDFSGMVIRSMPEWLEQVQESDKYGHL
                     VEPVLQLHHGRVSEEKGEFDVWENCLSLEWELRFGQLDGMSLPCELEAWAAIPEKEFY
                     RKQPETPEELARFAQGEPVLRMMARADIKHARVEMERCGDDPAPLARKRLNEAVRGLM
                     IRREQVGWENNYVSMPEHKAVNEPGWRSVVTFAMRT"
     gene            complement(3881..>5000)
                     /locus_tag="PLP58_20650"
     CDS             complement(3881..>5000)
                     /locus_tag="PLP58_20650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009959334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Hsp70 family protein"
                     /protein_id="HRJ11002.1"
                     /translation="DRALAEHIAPPSTDPALFITAAEAAKKRLSTEDTTRVEAPFYDG
                     ARSLSVEITRGEFEKIIRPLIERTRAHCLRALSDAGLEARDLQRVILVGGSTRIPLVR
                     ALAADIFDQEPDLSQNPDEAVALGAVIQAGILSGTLKNVLLLDVTPLSLGIETFGGLM
                     NVIIPRNTTLPCKAGEMFTNAVPNQKDMLIRVLQGERELARDNWELGRVIVPFPPGPK
                     GSARVGVQFTIDADGILHVLARDTATGADTLLEIQNTAVDVDDARVEAMISESVEHAF
                     EDMNERVWTEARLKAEELLAAVAAAMEQFGDAVSPEERAEIGTAAAAVRETLATPGHD
                     ARSLKAANQTLDDATQELAVRLVERAMEESLERRGLLG"
BASE COUNT          945 a         1291 c         1899 g          865 t
ORIGIN      
        1 tgaagggctc cgacgcgcgc aaggtgctgc tggcggactt gttgtggagg cggacggtgg
       61 tgtcgcagga atggctggcg gagaggttgc ggatgagttc ggcggcgaat gtgagccagc
      121 aactgaggcg gttggacaga aagcgcgcgc tgggcagggt gcccgaggcg atgagaaact
      181 ttctggagca acttgatgac agctccccgt gaacaacgca cgttttccgc gcgcagcagg
      241 gggcgcggga ccccgggggg gtttgaagga tgttggagaa cgctgaattt cgtttagaat
      301 ggaagccatg tacgaggaac ccaatgcaca ggagccggaa gaagagccaa gccaagcggc
      361 aaatagctgc caggcgaaaa atagccgtta ttaacgcaac acattcacaa tcaatgactc
      421 atttaccatt gtcgaaagcg cgtcatgccg cgccttggct gaactcggag ttgtcagggc
      481 gagccggaaa ccttgaggct gcagcacaac gcccggcatg gctgcggctg cgtagtcacc
      541 ccaatcgagc attgacaaag attgccaatg gcggcagggt ctggccaaag agaatatata
      601 tgccaacaca aaatgtgaat ttgacgcccg tcttggagag cttcgtgaag acgcaggtat
      661 cgctggggca cttcaacaat gccagcgagg tgcatcgctc ggcccttgcc gcgatggcgc
      721 ggcaggagga ggagcgggcg ttgcggttgg agcagttgcg gcgggaaatc caactcggta
      781 ttgaggatgt ggaggcgggg cggtgggcga agattggctc agtcaacggt ttgggtgcca
      841 tgctggatgg ctgtttggag aaggcggtcg agcgactgga actctcaaac gctgagccgg
      901 catcatgaat ttgaccagga cggccaaagc tgttgaggat atcgagtctt gtctgctgtg
      961 gtcggctgag aactttggaa tcccggcggc ccgtcgttac cgggcgctgc tggaagtggc
     1021 gctgctggcc attttggaag acccggaact gtgcggatcg aagctcatgg agggttttga
     1081 tggcaaggtg aggatttacc acctcaggca tagccgcaaa gcagctccgg tgggcggact
     1141 gatcgtcaag aatccaaggc atttcatcgt ctatcgaatt acgagcgaca gggcgattga
     1201 gcttctgcga gtgctgcacg agaggatgga ttttgaaacg cagatgaacg ggtaacgcca
     1261 ctgcccccag cggaagccgc ttcgctgctt ctcttggaaa gatgccagag tctggaatcc
     1321 gctagttgta tgatgaccgc tctgcactgg ctacgacttg cctgtctcct gctcttcggg
     1381 cttttcgcca ctacgacgac gagccaaggg gcgattcggg cgtatgattc tgaaggccgg
     1441 gttcctgccg caaagacaac agcgggtgac cttactgctg ctgagataaa gctaatccaa
     1501 ggaacggtgg atgcggctgg aaggccgctc gaagtcgtag ggacggaaaa ctgacgtttt
     1561 ccgctacggg ggaaagctga cgtactacgc cacggcgcgg ggagcggcga gtggaggaag
     1621 tcgtgaggct agctcgggga actgccgcca gggttggcag cgcgccgcct cgtgacgact
     1681 tggaataaat caggttaagg agactccagg acggatcgtt tttccctgcc ataaagccat
     1741 gaacgaggaa tccaatgcac aggagccgga agaagagcca agccaggcgg aggtggcgaa
     1801 gaggctgctg acgccggaag gccgcgcttt catggcggag atcatgcgca agacgctggc
     1861 cggagactac ggcgagctga cggaggaggt gatgcaaagc gcgaaagcgg ggctggagcg
     1921 ggtgcgctcg caggaagtga tggaggaggt gatgacccgc gtggaagcgc tgcgggagcg
     1981 gctgctggcc gcgcagcggg agaagccgga gtgcagcatg tgggccttca tgcgggacat
     2041 caatgaggaa atgaaggagc tcatggacct ggcgctggat gtgcatgagc cgcgccgctc
     2101 ggagatcatg ccgtgcctgc tttccttcca gcaacacatc gaagagttca tgaaaaagct
     2161 ctgacccgcc ccgcccatgc tctccttccg cgatgaagac tggattttcc gccacgggcc
     2221 gtcgcgcttt gaggcgtgga tcatgcccgg tcaccggaaa ccggcggatg tgatgtgggg
     2281 actggaactg cattacaaag cgggcggggc gcgctggcgg cgcgtggagg agcatgtgcg
     2341 cccggaggtg gatctcgcca tcagatcatg gaagctgaag agcgggcggt ggacggacct
     2401 gacggaggtc aaatattgga acaggaacct ggaagaccag aagcggaccg ggctgccgtg
     2461 gtttctgacg tgggagggcg gcatgctgga cctgtgccat cgtgcggagg cgggcgggaa
     2521 gcacgagttc tcccacgggt gcgaggtgtt ctggcaggtg cgggagcggg agggggggcg
     2581 ctttctggtg gagttggcgg cgggggtggg ccgggagcag ccggcggagg agaaggaact
     2641 ggtgctgccg gacggcacgc gggagaccgg ggagcgtgat ccatcggact tttggaggga
     2701 aaccgccgcc atctacgcgc tggagtgggt gccctttggc aaggtcaccg tgcgcgtgcc
     2761 gcgcaatgcg cgggatcatc tggcctgcgc ccgccaccgc gcgcatgagc tgctgggcat
     2821 ctccacacca ccggaggcct accatatgca ggatttcctg gtggtgcggg agggacgcga
     2881 ggaagaggag gctgtggcgg cgaacagcct gcatgacgat gtgtttgtga agctgcattt
     2941 ccacggctat cacgaggacg aatgacatga gctggatcca atggcgtgat gaaaccctgc
     3001 ccgcgaccca ggacgaggcg cgcatggagt tgaggctgca tgcgccggac atgggcggtc
     3061 cgcggcagtg ctggagtctg cggctcgcgc acgaagtgcc gtggccggat gacggggtga
     3121 agcggaagta ccgccatcag tgctggttca cagtggagct gctggagatg ggcgcgcagg
     3181 tgcgggacgt gcgggacttt tccggcatgg tgatccgctc gatgccggag tggctggagc
     3241 aagtgcagga gagcgacaag tacggacatc tggtggagcc ggtgctgcaa ctgcaccacg
     3301 ggcgcgtctc agaggaaaag ggggagtttg acgtgtggga gaactgcctg agcctggagt
     3361 gggagctgcg cttcggccaa ctggacggga tgagcctgcc gtgcgagctg gaggcgtggg
     3421 cggcgatccc ggagaaggag ttctaccgca agcagccgga gacgccggag gagctggcgc
     3481 gcttcgccca gggggagccg gtgctgcgca tgatggcgcg ggcggacatc aagcatgcaa
     3541 gggtggaaat ggagcgctgc ggggatgacc cggcgccgct ggcgcggaag cggctgaacg
     3601 aggcggtgcg cgggctgatg atcaggcggg agcaggtggg ctgggaaaac aattacgtgt
     3661 ccatgccgga gcacaaggcg gtgaatgagc ccggctggcg cagcgtggtg acctttgcca
     3721 tgcggacgtg acccgcgccg ggctcgtcgc tggctcgtag cggaagtcgt cagacttccc
     3781 ggatcgggag attggaggct ggagacgcgc caggaaagag aagcacgctt ccccaagccg
     3841 gggaaaactg acgttttcct ccacgggcac ggagcgcgtc tcagccgagc aggccgcgtc
     3901 tttcgaggga ctcctccatg gcgcgctcga cgaggcggac ggcgagctcc tgggtggcgt
     3961 cgtcgagggt ttggttggcg gctttgaggg agcgggcgtc gtgcccggga gtggcgaggg
     4021 tttcccgcac ggcggcggcg gcggtcccga tctcggcgcg ctcttcaggg ctgacggcgt
     4081 cgccaaactg ctccatggcg gcggcgacgg cggcgagcag ttcctcggct ttcaacctgg
     4141 cctcggtcca gacgcgctcg ttcatgtcct cgaaggcgtg ctcgacgctc tcgctgatca
     4201 tggcctcgac gcgggcgtcg tccacgtcca cggcggtgtt ttggatctcc aggagggtgt
     4261 cggcgccggt ggcggtgtcg cgcgcgagga cgtggaggat gccgtcggcg tcaatggtga
     4321 actggacgcc gacgcgggcg ctgcccttgg gtccgggcgg gaagggcacg atgacgcggc
     4381 cgagctccca gttgtcccgc gccagctcgc gctcgccctg aagcacgcgg atgagcatgt
     4441 ccttctggtt gggcacggcg ttggtgaaca tctcgccggc tttgcagggc agggtggtgt
     4501 tgcgcgggat gatgacgttc atgaggccgc cgaaggtctc gatgccgagg gagagcgggg
     4561 tgacatcgag caggaggacg tttttgagcg tgccgctgag gatgcctgcc tggatgacgg
     4621 cgccgagggc cacggcttcg tcggggttct gggaaaggtc cggctcctgg tcgaagatgt
     4681 cggcggccag ggcgcgcacg agcgggatgc gggtgctgcc gccgacgagg atgacgcgct
     4741 gcaggtcgcg cgcctctagc ccggcatcgc tgagggcgcg caggcagtgg gcgcgggtgc
     4801 gctcgatgag cgggcggatg attttttcaa actcgccgcg ggtgatctcg acggagaggc
     4861 tgcgcgcgcc atcgtagaag ggggcttcga cgcgggtggt gtcctcggtg gagaggcgct
     4921 tcttcgcggc ctcggcggcg gtgatgaaaa gggcggggtc cgtgctgggc ggggcgatgt
     4981 gttcggcgag ggcgcggtcc
//
LOCUS       DAOMYJ010000308         4980 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Prosthecobacter sp. isolate AMR_MDS_5629
            NODE_5844_length_4980_cov_19.333401, whole genome shotgun sequence.
ACCESSION   DAOMYJ010000308 DAOMYJ010000000
VERSION     DAOMYJ010000308.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867850
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prosthecobacter sp. (wastewater metagenome)
  ORGANISM  Prosthecobacter sp.
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Verrucomicrobiaceae; Prosthecobacter.
REFERENCE   1  (bases 1 to 4980)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4980)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.30
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:34:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,742
            CDSs (total)                      :: 4,684
            Genes (coding)                    :: 4,676
            CDSs (with protein)               :: 4,676
            Genes (RNA)                       :: 58
            tRNAs                             :: 55
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4980
                     /organism="Prosthecobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5844_length_4980_cov_19.333401"
                     /isolate="AMR_MDS_5629"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1965333"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..384
                     /locus_tag="PLP58_20655"
     CDS             <1..384
                     /locus_tag="PLP58_20655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ11003.1"
                     /translation="HEILRHGLGSDESTVIMFANDIIKRLDLVDALMSRYAGQNGGDV
                     VSQLRTLRHSFEDILLQHGVSEFDVDPGTEVDVPLRKRITIVESLPGKNKPRVVESCR
                     SGFIYSREEGHEVVLRKVEVRISSN"
     gene            427..1917
                     /locus_tag="PLP58_20660"
     CDS             427..1917
                     /locus_tag="PLP58_20660"
                     /inference="COORDINATES: protein motif:HMM:NF012242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp70 family protein"
                     /protein_id="HRJ11004.1"
                     /translation="MAEHVGIDLGTTLSGLAWLKPDGNPEIVPNADGERLTPSVVFFE
                     AHEDVKLVGSAARDGGDPGRTVFQIKRHMDDPEYLVKIDGKDWTPSEISALILAKLKR
                     ECSRIIGDINEVVITVPANFNELARKSTIAAAQMAGMKVRRLVNEPTAAAVYYAHTQG
                     VQGRILVYDLGGGTLDVTILQVDGDNIRILTSEGARHLGGSNFDELLLEAYAQQYNKQ
                     TGAGLYSDERHRRRVLQAVEDAKKMLSKLRRVNDTVANDQNSGLARIDLAREDFEAMI
                     RHMLTRTVMLVEQALDSAQLKASDIDHVVLVGGSTRIPKVKTMLEKLFGKSAVSCGNV
                     DECVALGAALFAKKSAKIQEVCNASYGTVAMVYNARTGEEKLMNSIVIPKNTPIPCSR
                     TQVYQTSEDNERIIEVDITQGEDEDAEYVDIIGRLTLEVPPNRPKGCEVAVTFSYDEN
                     QRVRALVLDKQSGRSREVAVSYKGAGVLSDEELKRRSAHLRQMRIE"
     gene            1958..2290
                     /locus_tag="PLP58_20665"
     CDS             1958..2290
                     /locus_tag="PLP58_20665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ11005.1"
                     /translation="MFKKILQSLTKSHSSRTAAAPAPAARASAPKTSPLAGIKNAPAK
                     SSAPQTPDELCGITPKMPKSEIMDQLKRLYRRHNRGASSLDPKVRARAEEMLHAIVVV
                     REKHFGQI"
     gene            2419..3744
                     /locus_tag="PLP58_20670"
     CDS             2419..3744
                     /locus_tag="PLP58_20670"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018969191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase"
                     /protein_id="HRJ11006.1"
                     /translation="MPALDDLLTCLRFPSVSTDPKHQPDVRACAAWLVEKLRGMGLTV
                     ELHETPGHPVIVAKNAHQPGRRTVLLYGHYDVQPPEPLNEWRTPAFEPTIRDARVWCR
                     GATDNKGQLMAHISGIAETLAQHGDLPVNLTILFEGEEEIGSPNLKPFLEAHREELAC
                     DVVAISDTGMVAPGVGTFTYGLRGIACLEARVHGPAIDLHSGIFGGAVANPATVAARL
                     AASLHDAEGRVAIPGFYDGVRDLQDWERAAWAELGDGEAETLELTGSPALFGEPGYTE
                     RERRWARPTAEVNGIGGGWQGAGSKTVIPREAFVKISFRLVPDQDPARILDLAEAHLR
                     AHMPAGARLEVHRGHTGQAYLMDPGSPFGQAARRALEKTFGGQVALIREGGSIPIVQA
                     FKDVLGVDTLLLGLALPDCQAHAPNENFPIASFEAGIRLNQELLRALAA"
     gene            complement(3907..4179)
                     /locus_tag="PLP58_20675"
     CDS             complement(3907..4179)
                     /locus_tag="PLP58_20675"
                     /inference="COORDINATES: protein motif:HMM:NF024600.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EF-hand domain-containing protein"
                     /protein_id="HRJ11007.1"
                     /translation="MKIIASILTVIAFAGITLDASAQEKKKQDPEKVFAKKDANGDGK
                     LSKEEFTKGAKDAAAAEKQFTARDKDKDGSVSKEEFMAAPKKKKDA"
     gene            complement(4555..>4980)
                     /locus_tag="PLP58_20680"
     CDS             complement(4555..>4980)
                     /locus_tag="PLP58_20680"
                     /inference="COORDINATES: protein motif:HMM:TIGR02603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HRJ11008.1"
                     /translation="VEAVMKLTGNPASGASLVMRCIMCHQIGEQGVEFGPSLQGWGIS
                     QPAEVIAQSLIHPSKDIAHGFDGHEIVTKDGVKIHGLVLAEGEVLIVRSMGGQTQYVP
                     RSRIASRKKLDRSMMLSAAQLGLTAQDVADITAWLRQAE"
BASE COUNT          851 a         1821 c         1505 g          803 t
ORIGIN      
        1 cacgagattc tccgccacgg cctgggcagc gacgagtcca ccgtcatcat gttcgccaac
       61 gacatcatca agcgcctcga cctcgtggac gcgctcatgt cccgctatgc cggccagaac
      121 ggcggcgatg tcgtctccca gctccgcacc ctgcgccact cgttcgagga catcctgctc
      181 cagcacggtg tcagcgagtt cgacgtggac cccggcaccg aggtggacgt gcctttgcgc
      241 aagcgcatca ccatcgtcga atccctgccc ggcaaaaaca aaccccgcgt ggttgaaagc
      301 tgccgctccg gctttatcta ctcgcgcgag gagggccatg aggtcgtcct gcgcaaggtc
      361 gaagtccgca tttccagcaa ctgagacccc tccagcaccc gcacattcct tttctcccct
      421 cacgttatgg ctgaacacgt tggcattgac cttggcacca ccctctccgg cctcgcctgg
      481 ctgaaacccg atggcaaccc cgaaatcgtc cccaatgccg acggcgagcg gctcactcct
      541 tcggtcgtct ttttcgaggc gcacgaggac gtgaaactcg tcggcagcgc cgcgcgcgac
      601 ggcggcgacc ccgggcgcac cgtcttccag atcaagcgcc acatggacga ccccgagtat
      661 ctcgtcaaaa ttgacggcaa ggactggact ccatccgaga tctccgcgct catcctcgcc
      721 aagctcaaac gcgagtgctc gcgcatcatc ggcgacatca acgaggtcgt catcaccgtc
      781 ccggccaact tcaacgaact cgcccgcaaa agcaccatcg ccgccgccca gatggccggc
      841 atgaaagtgc gccgcctcgt caacgaaccc accgcggccg ccgtctatta cgcgcacacc
      901 cagggcgtgc aggggcgcat cctcgtttat gacctcggcg gcggcacgct cgatgtcacc
      961 atcctccagg tcgatggcga caacatccgc atcctcacca gcgagggcgc gcgccacctc
     1021 ggcggcagca atttcgacga gctgctgctc gaagcctacg cccagcagta caacaagcag
     1081 accggcgccg gactctacag cgacgagcgc caccgccgcc gcgtgctcca ggccgtggag
     1141 gacgccaaaa aaatgctctc caagctgcgc cgcgtgaatg acaccgtggc caacgaccag
     1201 aactcgggcc tggcccgcat tgacctcgcc cgcgaggact tcgaggccat gatccgccac
     1261 atgctgaccc gcaccgtcat gctagtggag caggcgctgg actccgcgca gttgaaggcg
     1321 tccgacattg accacgtcgt cctcgtcggc ggctccaccc gcattcccaa ggtcaagacc
     1381 atgctcgaga agctcttcgg caagtccgcc gtctcctgcg gcaacgtgga cgagtgcgtc
     1441 gccctcggcg cggccctgtt cgccaagaag tcggcgaaaa tccaggaggt ctgcaacgcc
     1501 agctacggca ccgtggccat ggtctataac gcccgcaccg gcgaggagaa gctgatgaac
     1561 tccatcgtca tccccaaaaa cacccccatc ccctgctcgc gcacccaggt ttaccagact
     1621 tccgaggaca acgagcgcat catcgaggtg gacatcaccc agggcgagga cgaggacgcc
     1681 gagtatgtgg acatcatcgg ccgcctcact ctcgaggtgc cgccgaaccg ccccaagggc
     1741 tgcgaggtcg ccgtcacttt cagctatgac gagaaccagc gcgtgcgcgc cctcgtgctc
     1801 gacaagcagt ccggccgcag ccgcgaggtc gccgtcagct acaagggtgc cggcgtgctt
     1861 tccgacgagg aactcaagcg ccgcagcgcc cacctccgcc agatgcgcat cgagtgacgc
     1921 catcctccaa cacccctcat cccaccgcgc cgccgccatg ttcaagaaaa tcctccagtc
     1981 cctgaccaag tcccacagca gcaggacagc cgccgccccg gctcccgccg ccagggcgtc
     2041 cgcccccaag acctcgccgc tggccggcat caaaaacgcc cccgccaaat cctccgcgcc
     2101 gcagacgccc gatgagttgt gcggcatcac gccgaagatg cccaagagcg agatcatgga
     2161 ccagctcaaa cgactctacc gccgccacaa ccgcggcgcc tccagccttg acccgaaggt
     2221 ccgcgccagg gctgaggaaa tgctccacgc catcgtcgtc gtgcgcgaaa agcacttcgg
     2281 acagatttga ccgccagtgc cgcccctccc gtccgccagc gcgtcaggct cccccacagc
     2341 gggatgcgcc gtgacaggcc ccggacgcgg cgcagcccat ctccgagcag ccagtttcat
     2401 ttcccagcgt ccacactcat gcccgccctt gacgacctcc tcacctgcct gcgcttcccc
     2461 agcgtctcca ccgacccgaa gcaccagccc gacgtgcgcg cctgcgccgc gtggctggtg
     2521 gaaaagctgc gcggcatggg cttgaccgtg gagctgcacg agacgcccgg ccacccggtc
     2581 atcgtggcga aaaacgccca tcagccgggc cgccgcaccg tcctgctcta tggccactac
     2641 gatgtgcagc cgccggagcc gctcaatgaa tggcgcactc ccgccttcga acccacgatc
     2701 cgcgacgcgc gcgtctggtg ccggggtgcc acggacaaca aaggccagct catggcccac
     2761 atctccggca ttgccgaaac gctcgcccaa cacggggacc tccccgtgaa cttgacgatt
     2821 ctcttcgaag gcgaggagga gatcggcagc cccaatttaa aacccttcct cgaagctcat
     2881 cgcgaggaac tggcctgcga tgtcgtcgcc atctccgaca ccggcatggt ggcgcccggc
     2941 gtcggcacct tcacctacgg gctgcgcggc atcgcctgcc tggaggcgcg cgtgcatggg
     3001 ccggccatag atttgcactc cggcatcttc ggcggcgctg tggcaaaccc cgccaccgtg
     3061 gccgcccgcc tcgccgcctc actgcacgat gccgaaggcc gcgtggccat ccccggcttc
     3121 tacgacggcg tgcgcgacct ccaggactgg gagcgcgccg cctgggcgga gctgggtgat
     3181 ggcgaagccg agacgctgga actcaccggc tcccccgcgc tgttcggcga gcccggctac
     3241 accgagcgcg agcgccgctg ggcgcgcccc acggcggagg tcaacggcat cggcggcggc
     3301 tggcagggcg ccgggtcgaa gaccgtcatc ccgcgcgagg cctttgtgaa aatctccttc
     3361 cgtctcgtgc ccgaccagga ccccgcgcgc atcctcgacc ttgccgaggc gcacctgcgc
     3421 gctcacatgc ccgccggcgc ccggctcgaa gttcatcgcg gccacaccgg ccaggcctac
     3481 ctcatggacc ctggcagccc cttcggccag gccgcgcgcc gcgccttgga aaagaccttc
     3541 ggcggccagg tggccctcat ccgcgagggc ggcagcatcc ccatcgtcca ggccttcaaa
     3601 gacgtgctcg gtgtggacac cctgctgctc ggcctggccc tgccggactg ccaggcgcac
     3661 gcgccgaatg agaacttccc catcgccagc ttcgaggccg gcatccgcct gaatcaggag
     3721 ctgctgcgcg cgctggccgc ctgacctggg acgcgggcag cctgcctgcg cggcggaggg
     3781 agaggcgaca ccccccttcc ccatgcaccc gccagcggcg gcgcgcgcga ggccggaaag
     3841 aaaaacaccg cactcgcgcg gggcgggtgc ggtgttgagg tgaccgggct ggccggtggc
     3901 gccggatcag gcgtccttct tcttcttcgg agcggccatg aactcttcct tgctcacgga
     3961 gccgtccttg tccttgtcgc gcgcggtgaa ctgcttctca gcggcagccg cgtccttggc
     4021 gcccttggtg aactcctcct tgctcagctt gccgtcgccg ttggcgtctt ttttggcgaa
     4081 gaccttttct gggtcctgct tcttcttctc ctgggcggag gcgtcgagcg tgatgccggc
     4141 gaaggcgatc acggtgagga tggatgcgat gattttcatg tctgttggtg tgtgttgggt
     4201 tctggatacg ccggggtttg gttttggccc atccctatgc gatgcgcgct ttaacgcggg
     4261 cgccttcaca cagtttcagc tttttttcag tcccgcaaaa aagccgcccc tccggctacg
     4321 cgaggctgcc ccgcagcccg aatgccgggg aggaaacggc gggcggcccc cgcgctgcgg
     4381 ctccgccttt tcacgcggcg gcgtttgaga gcccggaccg gagttcaggc aacggacccg
     4441 tcacgcaccc ggccacgatc cggttccagt cctgaccagg cagtctgctc cgcgcaggcc
     4501 gcggatttgc acccgcagcc acgaagccgt gacgcgcact ggcgcgcgga ggggtcactc
     4561 ggcctggcgg agccaggcgg tgatgtcggc cacgtcctgg gcggtgaggc cgagctgggc
     4621 ggcgctgagc atcatgctgc ggtccagctt tttgcggctg gcgatgcggc tgcgcgggac
     4681 atactgggtc tgcccgccca tgctgcgcac gatgagcacc tccccttcgg cgaggacgag
     4741 tccgtggatc ttgacgccgt ccttggtgac gatctcgtgg ccgtcgaaac cgtgggcgat
     4801 gtctttgctc gggtggatga gggactgggc gatgacttcg gcgggctggc tgatgcccca
     4861 gccctggagg ctcgggccga actcgacacc ctgctcgccg atctggtggc acatgatgca
     4921 gcgcatgacg agggaggctc cgctggcggg gttgccggtg agtttcatga cggcttcgac
//
LOCUS       DAOMYJ010000309         4812 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Prosthecobacter sp. isolate AMR_MDS_5629
            NODE_6138_length_4812_cov_20.985705, whole genome shotgun sequence.
ACCESSION   DAOMYJ010000309 DAOMYJ010000000
VERSION     DAOMYJ010000309.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867850
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prosthecobacter sp. (wastewater metagenome)
  ORGANISM  Prosthecobacter sp.
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Verrucomicrobiaceae; Prosthecobacter.
REFERENCE   1  (bases 1 to 4812)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4812)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.30
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:34:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,742
            CDSs (total)                      :: 4,684
            Genes (coding)                    :: 4,676
            CDSs (with protein)               :: 4,676
            Genes (RNA)                       :: 58
            tRNAs                             :: 55
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4812
                     /organism="Prosthecobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6138_length_4812_cov_20.985705"
                     /isolate="AMR_MDS_5629"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1965333"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            253..930
                     /locus_tag="PLP58_20685"
     CDS             253..930
                     /locus_tag="PLP58_20685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ11009.1"
                     /translation="MLSYLLTFAAGFLCGAATWLLWMTLRFMADVKAASRAASRRLEE
                     AASGKDLEALQAVEACKNRLRWQKRVNPEWLPPLFEEVPRLVREIARIYHPAHPDPLL
                     APGLSEFARAVQLAAQDVSDFLETRSIGRLVDVSASTALKTWEMTHKIATHDAVQTAS
                     KWYKRILPVWQVVKYKSPMVWAGMAVSNVAARTLQPALIDIIARRVMDLYGGRAAAEM
                     PEKPVQN"
     gene            978..2318
                     /locus_tag="PLP58_20690"
     CDS             978..2318
                     /locus_tag="PLP58_20690"
                     /inference="COORDINATES: protein motif:HMM:NF012593.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dicarboxylate/amino acid:cation symporter"
                     /protein_id="HRJ11010.1"
                     /translation="MSSSKSSHGHHWPIVIALVSGAAAAVVLQPFVSPAEGDPAAWAG
                     KIIEVCRFFADLFLRALKMVIVPLVVASIISGIAGLKSLDGFARMGLKTWAYYLFGSL
                     VAVCVGLMFVNLVQPGLVDGAPNPTLRAAMDAALESEEAGEVGEIMKNAEGGTASLVD
                     IVRRAVPTNVIDACAKGDLLAIIMFSILFAVAMVLVPGGPPRALQDTFNQLTDVMTLI
                     TTWIMKFTPYAVFCLVIPAIATVGLGVLHNLVPYVLTVAGALLFHLLVIMPLVLRFIA
                     RVRPAAHFRNMEDPLLMSFSTASSTATIPVTMRSLMENSKVSEKVASFVVPLGATVNM
                     NGTALYECVVVVFAAQVLGIDLSLTQQFIVVLLALVSSIGVAGIPAASLVAIIIIMQN
                     AGFSEDMIKASLGLILTIDRPLDMARTTVNVFGDTVGAVLIARSEGEDVDGRVA"
     gene            complement(2324..2893)
                     /locus_tag="PLP58_20695"
     CDS             complement(2324..2893)
                     /locus_tag="PLP58_20695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009959338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C"
                     /protein_id="HRJ11011.1"
                     /translation="MNAAQMAAALKEKFGDAVLSTTEFRGEHTVQVAPTSLKLLLKYC
                     RDEMNFDYLVDISSLDHMGEEPRFEMVYELYGYGHLHHLRVKAKVAETQAVPTVSDIW
                     GTADWHEREVWDMMGIRFDGHPDLRRILMWEGYPYFPLRKEFPLAGKPSDLPEVGFTK
                     PAPMADGPFVTSAGAPDTVAREPRARRVG"
     gene            complement(2929..3462)
                     /gene="nuoB"
                     /locus_tag="PLP58_20700"
     CDS             complement(2929..3462)
                     /gene="nuoB"
                     /locus_tag="PLP58_20700"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009959337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoB"
                     /protein_id="HRJ11012.1"
                     /translation="MVAPAETHAAYDSKIEGNVVFTKLDSAINWMRKNSLWPMPMGLA
                     CCAIEMMAAACSRYDLSRFGMEVMRFSPRQADVMIVAGTVTYKMALAVKRIWDQMPEP
                     KWCIAMGACASSGGMYRSYAVLQGIDQLIPVDVYISGCPPRPEALLEGLMRLQHKIES
                     EHSLVEQKKELLAEMAS"
     gene            complement(3596..4519)
                     /locus_tag="PLP58_20705"
     CDS             complement(3596..4519)
                     /locus_tag="PLP58_20705"
                     /inference="COORDINATES: protein motif:HMM:NF024214.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HRJ11013.1"
                     /translation="MQKAFVLGAGLGTRLRPLTDQLPKPLIPVFHQPLISHAFDHLLG
                     AGVGEFVVNTHHLPHAYAGAFPESMHRRAPIRFRHESPERLETAGGIANVRDLLGEDP
                     FLVYNGDILTDLPLQRLLEEHEASGNLVTLALRSHGPALHIALDEQSGLVTDIRNLLG
                     TGQPGSLLFTGVYACQPEFHQWLTPGKVESVIPVFLRMIQAGARLGGVVIDEGRWWDL
                     GSRASYLEAHRELHESGSGPAIHPEARVSPDAVLTGLNVIGPGAVVESGAVLENCLLW
                     PDARVTASARLSRCIVRRGIQARDSHQDEDL"
     gene            complement(4667..4807)
                     /locus_tag="PLP58_20710"
     CDS             complement(4667..4807)
                     /locus_tag="PLP58_20710"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: protein motif:HMM:NF013436.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004329 - formate-tetrahydrofolate ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HRJ11014.1"
                     /translation="MRDFEIANGAGFLVALTGAMMRMPALPKVPAAERIQVSPDGAIT
                     GI"
BASE COUNT          792 a         1440 c         1712 g          868 t
ORIGIN      
        1 cggcgccggt ggcgaccgcg cgcgccgccc tttcggaggg cctccgcgcc gtcccttcgg
       61 cggtggtggc ggtggctatg gaggcggcgg cggatacggt ggcggcaagc ccggcggttt
      121 ccgcaagcct tatggcggca aacccggagg cttcaagccg cgccgttttg aggattgacg
      181 ccccgcgtgc cgctagcgca tgaggattct ttgcgttgag ttttcacgcg cccgcccgtt
      241 tgttggcgca ggatgttgtc ctatctcctg acattcgcgg cggggttcct ctgcggggcg
      301 gccacatggc tgctctggat gaccctgcgc ttcatggcgg atgtgaaggc cgccagccgc
      361 gcggcctcca ggaggctgga ggaggcggcc tcaggcaaag accttgaggc gctccaggcc
      421 gtggaggcgt gcaaaaaccg cctgcgctgg cagaaacgcg tgaacccgga atggctgccg
      481 cccctgttcg aggaggtgcc gcgcctggtg cgcgagatcg cccgcatcta ccacccggcg
      541 catcccgacc cgctgctggc tccgggtctg agcgagttcg cgcgcgcggt gcaactggcg
      601 gcgcaggatg tgtcggactt tctggagacg cgctccatcg ggcggcttgt ggacgtgagc
      661 gccagcacgg ctttgaagac ctgggaaatg acccacaaga tcgccacgca cgacgcggtg
      721 cagaccgcca gcaaatggta caagcgcatc ctgccagtgt ggcaggtggt gaagtacaaa
      781 tcgcccatgg tctgggcggg catggctgtc tccaacgtgg ccgcgcgcac gctgcagccc
      841 gcgctcattg acatcatcgc ccggcgtgtc atggacctct acggcgggcg cgcggcggcg
      901 gaaatgccgg agaagccggt gcaaaactga gtattcggct cttgccgcag gaggggaaaa
      961 gcggcataaa cgcgcccatg tcctccagca aatcctcgca cggccatcac tggccgattg
     1021 tcatcgcgtt ggtgtcaggc gccgccgcgg ctgtggtgct ccagcccttc gtcagccccg
     1081 cggagggaga cccggccgca tgggcgggca aaatcatcga agtctgccgc tttttcgccg
     1141 acctgttttt gcgcgctttg aaaatggtca tcgtgccgct ggtggtggcc agcatcatca
     1201 gcggcatcgc cgggctgaaa tctctggacg gtttcgcgcg catgggattg aaaacctggg
     1261 cctattatct ttttggcagc ctcgtggcgg tttgcgtggg cctgatgttc gtcaacctcg
     1321 tgcagccggg cctcgtggac ggcgcgccca accccaccct gcgcgcggcc atggacgcgg
     1381 cgctggaaag cgaggaggcg ggcgaggtgg gcgagatcat gaaaaacgcg gaaggcggca
     1441 ccgcctctct ggtggacatc gtgcgccgcg ccgtgcccac caatgtgata gacgcctgcg
     1501 cgaaggggga tctgctcgcc atcatcatgt tcagcattct tttcgccgtg gcgatggtgc
     1561 tcgtgcccgg cgggccgccg cgcgccctgc aggacacttt caaccagctc acggatgtga
     1621 tgacactcat caccacctgg atcatgaagt tcacgcccta cgcggtgttt tgcctggtca
     1681 tcccggccat cgccacggtg ggcctgggag tgctgcacaa tctggtgccc tatgtgctga
     1741 cagtggcggg cgcgctgctg tttcacctgc tggtgatcat gccgctggtg ctgaggttca
     1801 tcgcccgggt gagacccgcc gcgcattttc gcaacatgga ggatccgctt ttgatgtcct
     1861 tctccaccgc gtcctccacc gccaccattc cggtgaccat gcgcagcctg atggagaact
     1921 cgaaggtcag cgaaaaggtg gcgagctttg tcgtgccgct cggcgccacg gtgaacatga
     1981 acggcaccgc gctctatgag tgcgtggtcg tggtcttcgc cgctcaggtg ctgggcattg
     2041 acctgagttt gacacagcag ttcatcgtcg tcctgctggc gctggtctcc agcatcggcg
     2101 tggctggcat cccggcggcg agccttgtgg ccatcattat catcatgcaa aacgccggct
     2161 tttccgagga catgatcaag gccagcctgg gtctcatcct caccattgac cgcccgctcg
     2221 acatggcgcg caccacggtg aacgttttcg gcgacacggt cggcgcggtt cttatcgccc
     2281 gctcggaagg cgaggatgtg gacggcaggg tggcgtaggc ggatcagccc acgcgcctgg
     2341 cgcgcggctc gcgcgccacg gtgtcgggcg cgccggcgct ggtgacgaag gggccgtcgg
     2401 ccatgggggc gggtttggtg aagccgacct cgggcagatc gctgggcttg ccggcgaggg
     2461 gaaattcctt gcgcagcggg aagtaggggt agccctccca catgaggatg cggcgcaggt
     2521 ccgggtggcc gtcgaagcgg atgcccatca tgtcccacac ctcgcgctca tgccagtcgg
     2581 cggtgcccca gatgtcggag acggtgggca cggcctgggt ttcggccact ttggccttca
     2641 cgcgcaggtg atggaggtgg ccgtagccgt acagctcata gaccatctcg aatcgaggct
     2701 cctcgcccat gtggtcgagg ctggagatgt ccacgaggta gtcgaagttc atctcgtccc
     2761 ggcagtattt cagcaggagc ttgagcgagg tgggggcgac ctgcacggtg tgctctccgc
     2821 gaaattccgt ggtggagagc acggcgtcgc cgaatttttc cttgagggcg gcggccattt
     2881 gggcggcgtt catgagggcg ggggcgggaa ggcggtgagg gtgggaaatc aggaggccat
     2941 ctcggcgagc agttcttttt tctgctccac cagggagtgc tcggattcga tcttgtgctg
     3001 gaggcgcatg agcccttcga gcagggcctc cggacgcggc gggcagccgg agatgtacac
     3061 atccacgggg atgagctggt caatgccctg gagcaccgcg tagctgcggt acatgccgcc
     3121 gctggaggcg cacgcgccca tggcgatgca ccacttcggc tcgggcatct ggtcccagat
     3181 gcgcttcacg gcgagggcca ttttataagt gacggtgccg gcgacgatca tcacatccgc
     3241 ctggcggggg gagaagcgca tgacctccat gccgaagcgg ctcaaatcat agcggctgca
     3301 tgcggcggcc atcatctcga tggcgcagca ggccaggccc atgggcatgg gccagaggga
     3361 gttcttgcgc atccagttga tggcggagtc gagcttggtg aaaacgacgt tgccttcgat
     3421 ctttgagtcg taggcggcgt gagtttcggc gggggcgacc atgtcgtgcg ggggtaatac
     3481 ggggttcggc gagacttacg cgccgcgctg gcggcgggca acctggaatt tggcgggacg
     3541 gcggcggggc atccgcagat ttcacagatt tcgcagatga gttgcggaac tggctttaaa
     3601 gatcctcgtc ctggtggctg tcgcgcgcct ggatgccgcg gcggacgatg cagcgggaaa
     3661 gccgcgcgct ggcggtgacg cgggcgtcag gccagagcag gcagttttcc aggacggctc
     3721 ctgactcgac cacggcgccg gggccgatga cattcaaccc ggtcaaaacg gcgtccggcg
     3781 agacacgggc ctccgggtgg atggcggggc cgctgccgga ttcatgcagc tcgcggtgcg
     3841 cctccaggta ggaggcgcgg ctgcccagat cccaccagcg gccctcatca atgaccacgc
     3901 cgccgagacg cgcgccggcc tggatcatgc gcaggaaaac agggatgacg gactccactt
     3961 tccccggcgt gagccactgg tggaactcag gctggcaggc atacacgccg gtgaacagca
     4021 ggctgcccgg ctggccggtg ccgagcaggt tgcgaatgtc ggtgacgagg ccgctctgct
     4081 catccagggc gatgtgcaag gcggggccgt gactgcgcaa ggcaagggtg acgaggttgc
     4141 cgctcgcttc gtgctcctcc agcaggcgct gcaagggcag gtcggtgagg atgtcgccgt
     4201 tatagaccag gaagggatct tcccctagca ggtcgcgcac attggcgatg cctccggccg
     4261 tttccaggcg ctcagggctt tcatgacgga agcggatggg cgcgcgccgg tgcatggatt
     4321 ctggaaaagc tccggcataa gcatgaggaa gatggtgtgt gttcaccacg aactcaccca
     4381 cgcccgcgcc gagcaggtga tcgaaggcat ggctgatgag cggctgatga aaaacgggaa
     4441 tgaggggctt ggggagctgg tcggtcagcg gccgcagccg cgtgccgagt cccgcgccga
     4501 ggacgaaggc tttttgcacg cgcggcctgt aagcggaagc cgcgcgggcg caatcggatg
     4561 tttgcggaaa ggctggagcg aagcagcctg caggcggcat tgaggcttcg ctgcgggggg
     4621 agctggccgg acggctaaag cctggcacca gcgtcagacc ccagcgtcaa atgcctgtga
     4681 tggcgccgtc cgggctgacc tggatgcgct cggcggcggg gactttgggc agggcgggca
     4741 tgcgcatcat ggcgccggtg agggcgacga ggaagccggc gccgttggcg atttcgaagt
     4801 cgcgcacggt ga
//
LOCUS       DAOMYJ010000310         4805 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Prosthecobacter sp. isolate AMR_MDS_5629
            NODE_6151_length_4805_cov_22.966947, whole genome shotgun sequence.
ACCESSION   DAOMYJ010000310 DAOMYJ010000000
VERSION     DAOMYJ010000310.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867850
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prosthecobacter sp. (wastewater metagenome)
  ORGANISM  Prosthecobacter sp.
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Verrucomicrobiaceae; Prosthecobacter.
REFERENCE   1  (bases 1 to 4805)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4805)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.30
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:34:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,742
            CDSs (total)                      :: 4,684
            Genes (coding)                    :: 4,676
            CDSs (with protein)               :: 4,676
            Genes (RNA)                       :: 58
            tRNAs                             :: 55
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4805
                     /organism="Prosthecobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6151_length_4805_cov_22.966947"
                     /isolate="AMR_MDS_5629"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1965333"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..244
                     /locus_tag="PLP58_20715"
     CDS             <1..244
                     /locus_tag="PLP58_20715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ11015.1"
                     /translation="PVKPADENFSVFPQGALVRRRVCLNHNDAAGGLYPDLFTSHWLE
                     ALKSTHLRRWACFERHRAFTKALSFSNCARFAATHF"
     gene            complement(382..1029)
                     /locus_tag="PLP58_20720"
     CDS             complement(382..1029)
                     /locus_tag="PLP58_20720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007412903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HRJ11016.1"
                     /translation="MKRILLVDDHPIMRHGLAQLIRGEDGLEVCGEAGSAREGLEAVA
                     RHQPDLVVIDLTLPDKNGLELLKDIQAMHPGTLCLVLSMHDENMYGERALRAGARGYI
                     MKEAAADHLITAARKVLSGGIYVSEVIAGRMLEQVTGQRPGAASGIDSLTDRELEVLD
                     MIGRGVPTKTIAEKLCISARTVEAHRAHIKEKLGIHDGAALVRHAVRWVEERAGV"
     gene            complement(1026..2462)
                     /locus_tag="PLP58_20725"
     CDS             complement(1026..2462)
                     /locus_tag="PLP58_20725"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HRJ11017.1"
                     /translation="MMPATDPDLVQSLVDHAGMLVIGLDLEGRILWMSRGLEKISGRK
                     TAEVAGREWVETFVPEEHREEARKLCRGKLGSERSESHVKPLLVSNGPPRLVDWFHSD
                     LRDDKGNITGILCIGRDITELRAAREALEASEKRNRAVLETAVNAIITMDERCVIETA
                     NSATERIFGYTRDELVGSNISILMPQPYRDKHDSYVHAYMRTGERKIIGIGREVVGQR
                     KDGTIFPIDLSVGEALLPDGRRVFTGIIRDLTDRKRLEEKILQISEEEQHRIGQDIHD
                     DLCQQLAAIGCLAKVAQRQLARGGSPEAENLDEIVRLVTQANVRAREMSRGLMPVVLD
                     SAGLMEALKELAQGTVRIFRVSCPFRCERPVSVPDNKMATQLFRIAQEAVANAIKHSH
                     AARIEIALASGDDHVLLTIRDNGQGIPDNIPGKRTGMGLLTMTHRARMMGGALSVEPD
                     EFGGTIVRCRVPVPSPSKARPKSSPRKP"
     gene            complement(2571..3479)
                     /locus_tag="PLP58_20730"
     CDS             complement(2571..3479)
                     /locus_tag="PLP58_20730"
                     /inference="COORDINATES: protein motif:HMM:NF012791.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HRJ11018.1"
                     /translation="MKPYQRLIAAIDFTPSCRNALREAVRLAGGSGAAITAVHVMDEF
                     LVSELKRALSTDQASVRAEWEARLRKFVDDSDLGTPHVETEVRIGNAFFELSESCRVR
                     QADLLVMGAKGSRNEPNRVGVIAAKCVRKAPVDVLLVREDAQGPFKHLVACVDLSENS
                     AKAVSHALQIAAQDDASLDCLHVYQSALAMSLDYGGLAAPVASTMDDQGLRMWQSELD
                     KFIEPLKAAAPGVRVTSLVKERVNIREAILDHVTETHADMVVLGTRGKSGLRELLIGT
                     TAEKIVANAPCSILAVKPHILPEGQD"
     gene            3613..4764
                     /locus_tag="PLP58_20735"
     CDS             3613..4764
                     /locus_tag="PLP58_20735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009958169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HRJ11019.1"
                     /translation="MLDSSSRLRDFPGLADIAYLNTAAESIPPQCVHEALERYWRDKT
                     LGMRGREGHFSAVESCREISARMTGLKSREVSFCSCSSEAYNLLSSALDLGPQDEVVV
                     TDLDFPAGATPWLRAARPPVTRLWKNTGGVLDAACLQPLLNERTRLVQVSLVSFYNGH
                     RIAWQPLCAAVRALAPKALISVDVTQALGRVDLDCAAEADILISSTHKWSLGIHGGCV
                     IGVPERNAERLTTRAGGWYHLGNAFDADRFERADSKTGAASYSVGMPNFPALYALDAA
                     LRYVDGVSVAAISAHAGPLVAELRAGLLELGLEPMCPLDPALPTGILAFQHQRSAEMH
                     AALEKAGVHVMHHAGRIRLAVHGYNTREDITRALAALRAAVRGGKTTPH"
BASE COUNT          714 a         1514 c         1676 g          901 t
ORIGIN      
        1 ccccgtcaag cctgcggatg agaatttttc agtttttccg cagggtgctt tggtgaggcg
       61 ccgggtttgt ctgaatcata atgatgcggc aggaggactc taccctgatc tattcacgag
      121 ccattggctt gaggcattga aaagcacgca tctgcggcgt tgggcttgct ttgaaagaca
      181 tcgggcattc accaaggcac tgagcttttc aaactgcgcc cgctttgcag ccacgcactt
      241 ttgaacgtct ccctctcaaa gttcgtgaat agatcaggct agagcagggg agcgtttcgg
      301 aatgcgaatt ccatgagggt cactgcaagc ggccagacat cgcattgcct ggctgtcatt
      361 cggagacctt ttcaaacacg tctaaacccc ggcgcgctcc tcgacccatc tgacggcgtg
      421 gcggaccagg gcggcgccgt cgtggatgcc gagtttttct ttgatgtggg cgcggtgggc
      481 ctcgacggtg cgggcgctga tgcagagctt ttcggcgatg gtcttggtgg gcacgccgcg
      541 gccgatcatg tccagcacct ccagttcgcg gtcggtgagc gagtcaatgc cgctggcggc
      601 gccggggcgc tggccggtga cttgctcgag cattctgccg gcgatgacct cgctgacata
      661 gatgccgccg gagagcacct tgcgcgcggc ggtgatgagg tggtcggcgg cggcctcctt
      721 catgatgtag ccgcgcgcgc cggcgcgcag ggcgcgctcg ccgtacatgt tttcgtcatg
      781 catggagagg acgaggcaaa gggtgccggg atgcatggcc tggatgtcct tgagcagttc
      841 gaggccgttt ttgtccggga gggtgaggtc tatgacgacg aggtcgggct ggtggcgcgc
      901 cacggcttca aggccctcgc gcgcgctgcc ggcctcgccg cagacctcca ggccgtcctc
      961 gcctcggatg agctgagcga ggccgtggcg catgatgggg tggtcatcca cgaggaggat
     1021 gcgcttcacg gcttccgggg gctgcttttc gggcgggctt tggacggaga cggaacgggg
     1081 acgcggcatc ggacgatggt gccgccgaac tcgtcgggtt caactgaaag cgcgccgccc
     1141 atcatgcgcg cgcggtgggt catggtgagc aggcccatgc cggtgcgctt gcctgggatg
     1201 ttgtcgggga tgccctggcc gttgtcgcgg atggtgagca ggacgtggtc gtcgccggag
     1261 gcgagggcga tctcgatgcg ggcggcgtgg ctgtgcttga tggcgttggc gacggcctcc
     1321 tgggcgatgc ggaagagctg ggtggccatt ttgttgtccg gcacgctgac aggacgctcg
     1381 cagcggaagg ggcagctcac gcggaagatg cgcacggtgc cctgcgccag ctctttcaac
     1441 gcctccatga ggccggcgga gtcgagcacc acgggcatga ggccgcgcga catctcgcgg
     1501 gcgcgcacgt ttgcctgggt gacgaggcgc acgatctcgt ccaggttctc cgcctcgggg
     1561 ctgccgcctc gggcgagctg ccgttgcgcg acttttgcca ggcaaccgat ggcggcgagc
     1621 tgctggcaga ggtcgtcgtg aatgtcctgg ccgatgcggt gctgctcctc ctcgctgatc
     1681 tggaggattt tttcctcgag ccgcttgcgg tcggtgaggt cgcggatgat gccggtaaag
     1741 acgcgccgcc cgtcaggcag cagggcctcg cccacggaga ggtctatggg gaaaatggtt
     1801 ccgtccttgc gctggccgac gacctcgcgg ccgatgccga tgattttgcg ctcgccggtg
     1861 cgcatgtagg cgtggacgta gctgtcatgc ttgtcccggt agggctgggg catgagaatg
     1921 ctgatgttgc tgccgaccag ctcgtccctg gtgtagccga agatgcgctc ggtggcgctg
     1981 ttcgccgtct cgatgacgca gcgctcgtcc atggtgatga tggcgttgac ggcggtttcg
     2041 agcacggcgc ggttgcgctt ttcggaggcc tccagcgcct cgcgcgcggc gcgcagctcg
     2101 gtgatgtcgc gcccgatgca gaggatgccg gtgatgttgc ccttgtcgtc cctgaggtcg
     2161 gagtggaacc agtccaccag gcgcggcggg ccgttgctga cgagcagggg tttcacatgg
     2221 ctctcgctgc gctccgagcc gagcttgccg cggcagagtt tgcgcgcctc ctcgcggtgc
     2281 tcctcgggca caaaggtctc cacccattcc cgcccggcca cctctgcggt cttgcggcct
     2341 gagatttttt ccaggccgcg gctcatccag agaatgcggc cctccaggtc caggccgatg
     2401 acgagcatgc cagcgtggtc cacgaggctt tgcacgagat cagggtcggt ggcgggcatc
     2461 atccggcgcg gaggcgcggg ttcaccgtcc ccgccatgcc gcccggccat tccttgggaa
     2521 tgggccgggc cgctgagggc atgacgaggg ctgtggtccg ggatcatggc tcaatcttgc
     2581 ccctcgggca ggatgtgcgg cttcaccgcc aggatggagc agggggcgtt ggcgacgatt
     2641 ttttccgccg tggtgccgat caaaagctcg cgcaggccgc tcttgccgcg tgtcccgagc
     2701 acgaccatgt cggcatgggt ctcggtgacg tggtcgagga tggcctctcg gatgttgacg
     2761 cgctctttga caagacttgt gacgcgcacg ccgggcgcgg cggctttcag cggctcgatg
     2821 aatttgtcca gctccgactg ccacatccgc aggccctggt cgtccatggt ggaggccacg
     2881 ggcgcggcca gaccgccgta gtcgagggac atggccaggg cggactgata gacgtgcagg
     2941 cagtcgagcg aggcgtcatc ctgggcggcg atctggaggg cgtggctgac ggccttggct
     3001 gagttttccg agaggtccac gcaggccacg aggtgcttga aggggccttg cgcgtcctcg
     3061 cgcacgagca gcacgtccac gggcgccttg cgcacgcatt tggcggcgat gacgccgacg
     3121 cggttcggct cgttgcgcga gcctttggcg cccatgacga gcaggtccgc ctgacgcacg
     3181 cggcagctct cggacagctc gaagaaggcg ttgccgatgc gcacctccgt ctccacatgc
     3241 ggggtgccga ggtcggagtc gtccacgaat ttccgcagcc gcgcctccca ctcggcgcgc
     3301 acgctggcct ggtcggtgga gagcgcgcgc ttcagctcgc tgacgaggaa ttcatccatg
     3361 acatgcacgg cggtgatggc cgcgccggag ccgccggcaa ggcgcaccgc ctcgcgcagc
     3421 gcgttgcggc aggagggggt gaagtcaatg gcggcgatga gacgttggta gggcttcatg
     3481 tcggttggtt tgggttgagg gtaaaaccct gacggcaaaa ccgccccgcg tcacttgctt
     3541 tgttctgggc catgtttgcg gcgggtcctt gaaggaaaag ccccgcccgc gcgtgtttgc
     3601 tcatgccttc ccatgctcga ctcatcctcc cgccttcgtg atttccccgg ccttgcggac
     3661 atcgcctacc tgaacaccgc cgccgaaagc atcccgccgc aatgcgtgca tgaggccctg
     3721 gagcgctact ggcgggacaa gacgctgggg atgcgcgggc gcgagggcca cttttccgct
     3781 gtcgagtcct gccgggagat cagcgcgcgc atgaccgggc tgaaaagccg cgaagtctcc
     3841 ttttgctcat gcagttcgga ggcttacaac ctcctctcca gtgccctcga tctggggccg
     3901 caggacgagg tggtcgtcac cgatctcgac ttccccgccg gagccacacc ctggctgcgc
     3961 gccgcccgcc cgcctgtgac ccgcctgtgg aaaaacactg gcggcgtgct cgatgcggcc
     4021 tgcctccaac ccctgctcaa tgagcgcacc cgtctggtgc aggtctcgct tgtgagcttc
     4081 tacaacggcc accgcatcgc ctggcagccc ttgtgcgcgg ctgtgcgcgc cctggcccca
     4141 aaggcgctca tcagcgtgga tgtgacgcag gcgctcgggc gtgtggatct ggactgcgcg
     4201 gcggaggcgg acatcctcat cagcagcacg cacaaatgga gcctcggcat ccacggcggc
     4261 tgcgtcatcg gagtgccgga gcggaatgcg gagcgcctca ccacccgcgc cggcggctgg
     4321 tatcatctcg gcaacgcctt tgatgccgac cgcttcgagc gcgcggacag caagacgggc
     4381 gcggcgagct actccgtggg catgccgaac ttcccagcgc tctacgcgct cgatgccgcc
     4441 ctgcgttatg tggacggggt gagtgtggcc gccatcagcg cccatgccgg cccgcttgtg
     4501 gcggagctgc gcgccggcct tctggagctt ggcctggagc cgatgtgccc gctggacccg
     4561 gcactgccca cgggcatcct ggccttccag catcagcgat ccgcggaaat gcacgccgcc
     4621 ctggagaagg cgggcgtcca cgtcatgcac cacgccggcc gcatccgcct ggccgtgcat
     4681 ggttacaaca cgcgggagga catcacccgc gcgctagccg ccctgcgcgc cgcggtgcga
     4741 ggcggaaaaa cgaccccgca ttgacaagac gcgggcagct tccactttgc ccgcccccaa
     4801 ccccc
//
LOCUS       DAOMYJ010000311         4787 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Prosthecobacter sp. isolate AMR_MDS_5629
            NODE_6197_length_4787_cov_17.756340, whole genome shotgun sequence.
ACCESSION   DAOMYJ010000311 DAOMYJ010000000
VERSION     DAOMYJ010000311.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867850
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prosthecobacter sp. (wastewater metagenome)
  ORGANISM  Prosthecobacter sp.
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Verrucomicrobiaceae; Prosthecobacter.
REFERENCE   1  (bases 1 to 4787)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4787)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.30
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:34:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,742
            CDSs (total)                      :: 4,684
            Genes (coding)                    :: 4,676
            CDSs (with protein)               :: 4,676
            Genes (RNA)                       :: 58
            tRNAs                             :: 55
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4787
                     /organism="Prosthecobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6197_length_4787_cov_17.756340"
                     /isolate="AMR_MDS_5629"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1965333"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..400
                     /locus_tag="PLP58_20740"
     CDS             <1..400
                     /locus_tag="PLP58_20740"
                     /inference="COORDINATES: protein motif:HMM:NF024503.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TonB C-terminal domain-containing protein"
                     /protein_id="HRJ11020.1"
                     /translation="PIPPPKARATSTRSTPPPSLRPVVTLMDIAKLKDNSTQGMDAVL
                     VEMQGAFMDAWQPPEMTSANGRALSVVMRLLLRKDGTLGGTVLEKSSGRDALDASVLN
                     AAAKVKKISTELPANFPEHGYDVEATFLIE"
     gene            401..1591
                     /locus_tag="PLP58_20745"
     CDS             401..1591
                     /locus_tag="PLP58_20745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ11021.1"
                     /translation="MRLPFLLACLLVVSAAEAFELPGWLRLPGAEKREKSAAAPRRLG
                     ISEFTGGTGGSARRSLERELAAASGIVVTVEQPEFQVSGSSVGGRVTGRLVDGAGKVL
                     FERTYAAPGLEENLRLLADDIIYSITGKPGLATSRIAFVSDRGGTRQIYLCDPQGGEV
                     QQLTRHAHGAVSPSLSPDGSAIAFTSYRGGFPSVMLLDLGLGWERSVTDTAGINSGAA
                     FSPEGRRLALVMSFLGNPEIFVTDLNTNTAACVSDTLGVPSGPAWHPDGRHIIFSDDR
                     GRGPRLYLAEIPEKEDGEARLYLWRTGHSFCTDPEFSPDGKQVAFTARVHGRSAVVMR
                     DFPSGRSRVVQGEGAEHPSWSPNGRHLCYTQHGALYIHDLVSGQRRVVLSGHGTITEP
                     RWMR"
     gene            1588..2046
                     /locus_tag="PLP58_20750"
     CDS             1588..2046
                     /locus_tag="PLP58_20750"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HRJ11022.1"
                     /translation="MKVLLTALPCLLLLPACGHLGGLLPEADTGGLVYALGPREGFTS
                     PLAAALKPACPPLEFPADGHALTGAHEKILRDFMAELGGDLEKRHLIVGHTPVELPGG
                     YARSLSERRAQAARQFLIENSMPAASLQTLGLGHDSPAGATGHRVILYAQ"
     gene            2105..3238
                     /gene="moeB"
                     /locus_tag="PLP58_20755"
     CDS             2105..3238
                     /gene="moeB"
                     /locus_tag="PLP58_20755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018968955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008641 - ubiquitin-like modifier
                     activating enzyme activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-synthase adenylyltransferase MoeB"
                     /protein_id="HRJ11023.1"
                     /translation="MQDLTQAELQRYARHLAIPGFGREAQEKLKAASVLCIGAGGLGS
                     PVALYLAAAGIGRLGLVDPDIVDLSNLQRQILYGGADVGRPKLDAARERLLQTNPHVE
                     VVPHPEHFTAANARQIAAGYDIILDGTDNFPTRYLSNDVAVWQRKPNIYGSILRFEGQ
                     VSVFAPHLGGPCYRCMAPAPPKPGLVPTCAEGGVLGVLPGLIGTMQALEVIKLVTGIG
                     APLIGRLLHVDTLAPRYRTFTLPRDPDCPVCGAHPTITEPIDYTGFCGMTPTPDVPTL
                     TVHELHQMRQRGDSHFLLDVREPDEHATARISGSTLIPLGQVTDRAAEIPRDTRVLVH
                     CKLGGRSARAVAALRDLGFDNVWNIAGGITAWSREIDTTVPQY"
     gene            3305..4096
                     /locus_tag="PLP58_20760"
     CDS             3305..4096
                     /locus_tag="PLP58_20760"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HRJ11024.1"
                     /translation="MSSLLRVQNASFVRGGRRILQDITWEVRPGEHWCVLGPNGCGKT
                     SLINLITGYEPATEGDIQIGADHFGGSDWREVRKRVGLVTNTLTTFIEMEEPVLHVIA
                     SGRDAKLNLWQPPPPAWRRQAASLLDAVGGAHLRDSLWGTLSQGERQKVLICRALMAR
                     FEVLILDEPCAGLDPVAREQFLDWMAGIATWEEAPSLVLVTHHVEEILPCLSHVLLLR
                     AGRVFAAGEKSATLTSANLGAVYGAPVKLTRRQGRYTLKVSGGAA"
     gene            complement(4198..>4787)
                     /locus_tag="PLP58_20765"
     CDS             complement(4198..>4787)
                     /locus_tag="PLP58_20765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ11025.1"
                     /translation="ALAGVIEGDPGFTQSVAECAAQEARALLETLRQEAPGQRLGTLV
                     LGCTHYPLARGELDAAFTTLRADPRFSPLLEDTLAYVDPAEWTARELFRELAKAGLRL
                     KAGETCDAGAHSFYLSVPNPACSGVKLAEDGSLDRDYKYTRLPGDLMREDTLNTPLKA
                     AALPAVGRNLVEKRLPEVWRALGGSKNLDASSRVE"
BASE COUNT          764 a         1754 c         1516 g          753 t
ORIGIN      
        1 gcctattcca cccccgaagg cccgggcaac ctctacaaga tcgaccccgc caccgtcgct
       61 gcgtcccgtg gtgacgttga tggacatcgc caaactcaag gacaacagca cccagggcat
      121 ggacgcggtg ctggtggaga tgcagggggc attcatggat gcctggcagc cgccggagat
      181 gaccagcgcc aacggccgcg ccctctccgt ggtgatgcgt cttttgctgc gcaaggacgg
      241 cacactgggc ggcacggtgc tggagaagtc ctccgggcgc gatgccctgg acgcctccgt
      301 tttgaacgcg gccgcgaagg tgaaaaaaat atccaccgag ctgcctgcaa acttcccgga
      361 gcacggctat gatgtggagg caaccttcct gatcgaataa atgcgccttc ccttcctgct
      421 cgcctgcctc ctcgtcgtct ctgcggcgga ggcctttgag ctgccgggct ggctgcggct
      481 gccgggcgcg gaaaaacggg aaaaatccgc cgccgcgcca cgcaggctgg gcatcagcga
      541 gttcaccggc ggcaccggcg gcagcgcgcg ccgcagcctg gagcgcgaac tggcggcggc
      601 cagcggcatc gtcgtcaccg tcgagcagcc ggaattccag gtcagcggat catccgtggg
      661 cgggcgagtc accggccggc tcgtggacgg cgcggggaag gtgcttttcg agcgcaccta
      721 cgccgctccc ggcctggagg aaaacctgcg cctgctggct gatgacatca tctactccat
      781 caccggcaag cccgggctgg ccaccagccg catcgccttt gtcagcgacc gcggcggcac
      841 ccggcagatt tacctgtgcg acccccaggg cggggaggtc cagcagctca cccgccacgc
      901 tcacggagcg gtctcgccca gcctctcgcc ggacggctcc gccatcgcct tcaccagtta
      961 ccgcggcggt ttcccctccg tcatgctgct tgatctcgga ctcggctggg agcgctccgt
     1021 gacggacacc gccggcatca atagcggcgc cgccttttct cccgaaggac gcaggctggc
     1081 cctggtcatg agtttcctcg gcaaccccga aatcttcgtc accgacctca acaccaacac
     1141 cgccgcctgc gtcagcgaca ccctgggcgt gcccagcggc cccgcctggc atcccgacgg
     1201 gcggcacatc atcttctccg acgaccgcgg gcgcggaccc aggctctacc tggccgagat
     1261 acccgaaaag gaggacggcg aggcgcggct ctacctctgg cgcaccggac actccttctg
     1321 caccgacccg gaattctcac cggatggcaa acaggtggcc ttcacggccc gcgtgcatgg
     1381 caggtccgcc gtggtgatgc gggacttccc ttccggccgt tcgcgcgtcg tgcagggcga
     1441 aggcgctgag cacccctcat ggagtccgaa cgggcgccac ctctgctaca cccagcacgg
     1501 cgcgctctac atccacgacc tggtctccgg ccagcgccgt gtggtgctct ccgggcacgg
     1561 caccatcacc gaaccccgct ggatgcgatg aaagtcctcc tcacagcact cccctgcctg
     1621 ctgctcttgc ccgcctgcgg ccacctcggc ggactgctgc ccgaggccga caccggcggc
     1681 cttgtctatg cgctcggccc gcgcgaaggc ttcaccagcc cgctggccgc ggccttgaag
     1741 ccagcctgcc cgccgcttga gttcccggcg gacgggcacg cgctcacagg cgcgcatgag
     1801 aaaatcctgc gcgacttcat ggcggagctt ggcggggatt tggaaaaacg acacctcatc
     1861 gtcgggcaca cccccgtgga gctgccgggc ggttacgcgc gctccctctc agagcggcgc
     1921 gcccaggcgg cccggcagtt cctcatcgaa aacagcatgc ccgccgcaag cctgcaaacc
     1981 ctcggactcg gccacgactc tcccgccggc gccaccggcc accgcgtcat cctttacgcg
     2041 cagtgacggc ccgcgccaaa gccgggttgc catcccgcag cgctggtgct cacttggctc
     2101 ctgcatgcaa gatctcaccc aggctgaact tcagcgctac gcccgccacc tggccattcc
     2161 aggtttcggg cgcgaggcgc aggagaagct caaagccgcc agcgtactct gcatcggcgc
     2221 cggcggtctc ggctcccccg tggcgctcta cctagccgcc gcaggcatcg gcaggctcgg
     2281 ccttgtggat cctgacatcg tggacctcag caacctccag cgccagatcc tctatggcgg
     2341 cgcggatgtg ggccgcccca agctcgacgc cgcgcgcgag cgcctcctcc agaccaaccc
     2401 gcacgtcgag gtcgtccccc accctgaaca cttcaccgcc gccaacgccc gccagatcgc
     2461 agccggttac gacatcatcc tcgacggcac cgacaacttc cccacccgct acctctccaa
     2521 tgatgtggct gtctggcagc gcaagcccaa catctacggc agcatcctgc gctttgaagg
     2581 ccaggtctcc gtctttgccc cgcatctcgg cggcccctgc taccgctgca tggcgcccgc
     2641 cccgcccaag cccggcctcg tgcccacctg cgccgagggc ggcgtgctcg gcgtcctgcc
     2701 cggcctcatc ggcaccatgc aggcgctgga ggtcatcaag ctcgtcaccg gcatcggcgc
     2761 gcccctcatc ggccgcctcc tgcatgtgga caccctggcc ccgcgctatc gtactttcac
     2821 cctcccgcgc gaccctgact gccccgtctg cggcgcgcac cccaccatca ctgaacccat
     2881 agactacaca ggcttctgcg gcatgacacc aacacccgac gtccccaccc tcaccgtcca
     2941 cgaactgcat caaatgcgcc agcgcggcga ctcccacttc ctgctcgacg tgcgcgagcc
     3001 cgacgagcac gccaccgcgc gcatcagcgg ctccacgctc attcccctcg gccaggtcac
     3061 cgaccgcgcc gctgaaatcc ccagggacac ccgggtgctc gtccactgca agctcggcgg
     3121 gcgcagcgcg cgcgccgtcg cagccctgcg cgacctcggc ttcgacaacg tctggaacat
     3181 cgccggcggc atcaccgcct ggtccaggga gatagacacc accgtgcccc agtattagcg
     3241 ccggcctgcc cgccctgcgg cgtgtcttgc cagggcgggc ctccgcatgt agggacggcg
     3301 gcacatgagc agccttctcc gggtccaaaa cgccagcttc gtgcgcggcg gccgccgcat
     3361 tttgcaggac atcacctggg aggtgcggcc gggggagcat tggtgcgtac tgggaccgaa
     3421 cggctgcggg aagacctcgc tcatcaacct gatcacgggc tacgagccgg cgacggaggg
     3481 cgacatccag atcggcgcgg accatttcgg cggcagcgac tggcgggagg tgcgcaagcg
     3541 cgtgggcctg gtgaccaaca cgctgacgac tttcatcgag atggaggagc ctgtgctgca
     3601 cgtcatcgcc agcgggcgcg atgccaagct caatctctgg cagccgccgc cgcccgcgtg
     3661 gcggcggcag gctgcgtccc tgctcgacgc ggtgggcggc gcgcacctgc gcgacagcct
     3721 gtggggcacg ctctcgcagg gggagcggca gaaggtgctc atctgccggg cgctaatggc
     3781 gcgcttcgaa gtgctcatcc tcgacgagcc atgcgcgggg ctggaccccg tggcgcgtga
     3841 gcaattcctg gattggatgg cgggcatcgc cacctgggag gaggcgccct cgctggtgct
     3901 ggtgacgcac catgtggagg agatcctgcc ctgcctgagc catgtgctgc tgctgcgcgc
     3961 ggggcgcgtc ttcgcggcgg gagaaaagtc cgccacgctc accagcgcga acctgggcgc
     4021 cgtctatggc gcgccggtga aactcacgcg gaggcagggg cgctacacgc tcaaagtcag
     4081 cggcggcgcg gcgtgaggat ggagcttttt gcgaagcggc attgcgtgag tgcggctcgc
     4141 atcttcaggc ttcagcgcgt catgccagcc tgacgatccg attcattccc atcgaagcta
     4201 ttcaactctc gaactggcat caagattttt gctcccgccc agtgcgcgcc agacttcggg
     4261 cagtcttttc tccacgagat tgcgccccac ggcgggcaga gcggcggctt tcaggggagt
     4321 gttcagtgtg tcctcgcgca tcaggtcgcc gggcaggcgt gtgtatttgt agtcccggtc
     4381 cagggagcca tcctccgcga gtttgactcc ggaacaggcg ggattgggga cagagaggta
     4441 aaaggagtgc gcgccggcat cgcaagtctc gcccgctttg aggcgcaggc cggctttggc
     4501 cagctcccgg aacagctcgc gcgcggtcca ctcggcaggg tccacatagg ccagggtgtc
     4561 ctccagcagg gggctgaagc gcgggtccgc gcgcagggtg gtgaaggcgg cgtccagctc
     4621 gccgcgcgcc agcgggtaat gcgtgcagcc cagcaccagg gtgccgagcc tttggccggg
     4681 agcctcctgg cgcagcgtct ccagcagcgc gcgcgcctcc tgcgcggcgc actcggccac
     4741 gctttgggtg aagccggggt cgccctcgat gactcccgcg agcgccg
//
LOCUS       DAOMYJ010000312         4769 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Prosthecobacter sp. isolate AMR_MDS_5629
            NODE_6229_length_4769_cov_16.935087, whole genome shotgun sequence.
ACCESSION   DAOMYJ010000312 DAOMYJ010000000
VERSION     DAOMYJ010000312.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867850
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prosthecobacter sp. (wastewater metagenome)
  ORGANISM  Prosthecobacter sp.
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Verrucomicrobiaceae; Prosthecobacter.
REFERENCE   1  (bases 1 to 4769)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4769)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.30
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:34:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,742
            CDSs (total)                      :: 4,684
            Genes (coding)                    :: 4,676
            CDSs (with protein)               :: 4,676
            Genes (RNA)                       :: 58
            tRNAs                             :: 55
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4769
                     /organism="Prosthecobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6229_length_4769_cov_16.935087"
                     /isolate="AMR_MDS_5629"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1965333"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(38..805)
                     /gene="pyrH"
                     /locus_tag="PLP58_20770"
     CDS             complement(38..805)
                     /gene="pyrH"
                     /locus_tag="PLP58_20770"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012420358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033862 - UMP kinase activity [Evidence
                     IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HRJ11026.1"
                     /translation="MCQIENHMGDTNTGTRKFRRVLLKLSGEALREPGSTDNISPPIV
                     EDIAAQIKAAHQTGIEVAVVVGGGNFWRGVSASNKGMERATADYMGMLATVMNSLAVQ
                     SMLEAMDVPTRVQSAIEMKNVAEPFIRRVAMRHLELGRVVIFAAGTGNPFFSTDTTAA
                     LRASEIGAQCVFKATKVDGIYDKDPSRHADAVRFENVSFHECLTRQLKVMDATAFSLC
                     MENDMPVIVFSMNEPGNIRRALTGETLGTLVNTAGKP"
     gene            918..2018
                     /locus_tag="PLP58_20775"
     CDS             918..2018
                     /locus_tag="PLP58_20775"
                     /inference="COORDINATES: protein motif:HMM:NF013567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HRJ11027.1"
                     /translation="MNTTHCRWGILGAAFIARKNWQAIRDAGNAALIAVASRDLERAR
                     AFITECQASAPHARTPEAVEGYEALLARKDIDAVYLPLPTGLRKEWALRAAAAGKHVL
                     VEKPAGRDAAEVAEIIAACEKHGVQFMDGVMFMHGARLRELRGVLDREAGRVRHVAAQ
                     FSFLADEGFEGGNIRAHGALEPLGCLGDLGWYCLRFTLWAMNYAQPEVVTGRVHRWTK
                     GGPESPPVPLEFSGTLAFADGASASFYCSFTAANAQWAVVSGEKALLQVTDFVLPFSG
                     ARTAYSVTRSAFFLDGCRSDMREGRAERWLDEPSSNAPGAQEAEMFRVFSGLVLEGAR
                     DAFWPRASLLTQRVLDACLESARADSRVVRLS"
     gene            2052..2804
                     /locus_tag="PLP58_20780"
     CDS             2052..2804
                     /locus_tag="PLP58_20780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009960312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein A"
                     /protein_id="HRJ11028.1"
                     /translation="MPRLSRRVEDKDYKVKLEIFEGPLDLLLYLIKKDEIGIYDVSIE
                     RITRQYLEYMSTFKMLNVELASEFIVMAANLMYIKSRELLPQSQQPPEEDAEEEDPRW
                     ELIRQLVEYKKFKDAAQFLGTQEVKADEFFATNPELPDLEAPVAPAIGQVGIFDLIRA
                     FQRVLKRFENTTDIREIVSDRFTVADKIEHLLEAIPAGGRARFESLFSDAASRVEVIV
                     TFLAMLELMKLNHLQVEQEGMLGEIIVLRPAQ"
     gene            2905..3570
                     /locus_tag="PLP58_20785"
     CDS             2905..3570
                     /locus_tag="PLP58_20785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009961350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HRJ11029.1"
                     /translation="MEEQPDIPEEVKKEKGLWEKTRKSLIERLNNWEDQRTWNEFYQT
                     YWRLIYSVATKAGLTREEAFDVIQETIIAIARQVQKGQYDPRAGSFKAWLLQMTRWRV
                     LDVFRARKRQPSLADQGNADSEETSNLALERLSNEKDNLLEKIWDREWRDNITAAALE
                     KVKAKVSPRQFQIFDCYVMKGWGVKKTSEALGINAAQVYLAKHRVGALVKKEIQALEH
                     TML"
     gene            complement(3683..>4769)
                     /locus_tag="PLP58_20790"
     CDS             complement(3683..>4769)
                     /locus_tag="PLP58_20790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ11030.1"
                     /translation="GGHARLTVTTGGKVTGSLLERGKKFPITGQLDVQPGGAGASLTA
                     PVKRATGQSRVILKVPLFANLAALTGSLDEEGTAESTDLNGWQCPFSAKNKTTLTGLH
                     NLALTGPGGLDSALAPDGDSFAVVTLGATGSAKWAGVLADGATFTAAHLLGRNAGAEA
                     AQLHLHAPLYAGTGSVQGGARVNDLPGAWDAVPGLEMDWRKLAQLKPARSYTNGFGPL
                     ALDVQGARFVKAAPGQRLTGLAPAMVLELRSGGLGANEFQCNAAISDKNIVTLGAVAG
                     PPGTTQITGLKVAVAAASGRISGGFTLIENPGTPQQTKRAVKLAGIVVPVAGGGGAGH
                     FTLAENPGPGQKASATPIRGGRVILRE"
BASE COUNT          776 a         1426 c         1757 g          810 t
ORIGIN      
        1 gtttgcctct acggggcgcg cggggggaat ggggcgctca gggtttgccg gcggtgttga
       61 cgagggtgcc gagggtctcg ccggtgaggg cgcggcggat gttgccgggc tcgttcatgc
      121 tgaagacgat gacgggcatg tcattctcca tgcacaggga gaaggcggtg gcgtccatca
      181 ccttgagctg gcgggtcagg cactcgtgga agctgacgtt ttcaaagcgc acggcgtcgg
      241 cgtgcctgga ggggtccttg tcgtagatgc cgtccacctt ggtggctttg aagacgcatt
      301 gcgcgccgat ctcggaggcg cgcagggcgg cggtggtgtc ggtggagaag aaggggttgc
      361 cggtgccggc ggcgaagatg acgacgcggc cgagctccag atggcgcatg gcgacgcggc
      421 ggatgaaggg ctcggcgacg tttttcatct cgatggcgct ctgcacgcgg gtgggcacgt
      481 ccatggcctc gagcatggac tggacggcga gggagttcat gacggtggcg agcatgccca
      541 tgtagtcggc ggtggcgcgc tccatgcctt tgttgctggc gctgacgccg cgccagaagt
      601 tgccgccgcc gacgacgacg gccacctcga tgccggtctg gtgcgcggct ttgatctgtg
      661 cggcgatgtc ctcgacgatg ggcggggaga tgttgtcggt gctgccgggc tcgcgcaggg
      721 cctcgccgct gagtttgagt aatacacgcc tgaattttcg agtgccggtg ttggtgtcgc
      781 ccatgtgatt ttcgatttgg cacatgaaac aggatgcgca aggcgatgcc aagatttttc
      841 cgcaaagaga gggcggcttg acttgcctgg ggctgtcttg aagactgcgc gatcctccaa
      901 cccgtcttcc caatcccatg aacacgaccc attgccgctg gggcatcctc ggcgcggcct
      961 tcatcgcccg caagaactgg caggccatcc gcgatgccgg gaatgccgcg ctcatcgcgg
     1021 tggcgagccg cgatctggag cgggcgcgcg ctttcatcac cgaatgccag gccagcgcgc
     1081 cgcacgcccg gacgcccgag gcggtggagg gctacgaggc gctgctggcg cggaaggaca
     1141 ttgacgcggt gtacctcccg ctgcccacgg gcctgcggaa ggagtgggcg ctgcgcgcgg
     1201 cggcggcggg gaagcatgtg ctggtggaga agccggcggg gcgggacgcg gcggaggtgg
     1261 cggagatcat cgcggcctgt gaaaagcacg gggtgcagtt catggatggt gtgatgttca
     1321 tgcacggggc gcggctgcgg gagctgcgcg gcgtgctgga cagggaggct ggccgggtgc
     1381 ggcatgtggc cgcgcagttc agcttcctgg cggacgaggg ttttgagggc gggaacatcc
     1441 gcgcgcacgg ggcgctggag ccgctgggct gcctggggga tctgggctgg tactgcctgc
     1501 gcttcacgct ctgggccatg aactacgcgc agccggaggt ggtgacgggg cgcgtccaca
     1561 ggtggacgaa gggaggaccg gagtcgccgc cggtgccgct ggagttctcc ggcacgctgg
     1621 ccttcgcgga cggggcgtcg gcgtcgtttt actgctcgtt cacggcggcg aacgcgcagt
     1681 gggccgtggt cagcggggag aaggcgctgc tgcaggtgac ggatttcgtg ctgcccttct
     1741 ccggcgcgcg gacggcgtac agcgtgacgc gcagcgcgtt cttcctggac gggtgccgct
     1801 cggacatgcg cgaggggcgg gcggagcgct ggctggacga gccttcgagc aacgcgcccg
     1861 gcgcgcagga ggcggagatg ttccgcgtct tttcaggcct ggtgctggag ggcgcgcgcg
     1921 acgcgttctg gccgcgcgcg agcctgctga cgcagcgcgt gctggacgcc tgcctggagt
     1981 ccgcgcgcgc ggattcgcgg gtggtgaggc tttcctgatg cgggcggttt ttttcttctg
     2041 gcagggcgcg gatgccgcgt ttatctcggc gggtggaaga caaggattac aaggtcaaac
     2101 tggagatttt cgagggaccc ctcgacctgc tgctgtatct catcaaaaag gacgagatcg
     2161 gcatctatga cgtgtccatc gagcgcatca cgcgccagta tctggagtac atgagcacgt
     2221 ttaaaatgct caatgtggag ctggccagcg agttcatcgt catggcggcg aacctgatgt
     2281 acatcaagag ccgggagctg ctgccgcagt cccagcagcc gccggaggag gacgcggagg
     2341 aggaggatcc gcgctgggag ctgatccgcc agctggtgga gtacaagaag ttcaaggacg
     2401 cggcgcagtt cctcggcacg caggaggtga aggcggacga gtttttcgcc accaacccgg
     2461 agctgccgga cctggaggcc ccggtggcgc cggccatcgg gcaggtgggc atcttcgacc
     2521 tcatccgcgc cttccagcgc gtgctcaagc gcttcgagaa cacgaccgac atccgcgaga
     2581 tcgtcagcga ccgcttcacg gtggccgaca agatcgagca cctgctggag gccatcccgg
     2641 ccggcgggag ggcgcggttc gagagcctgt tcagtgacgc cgccagccgc gtggaggtca
     2701 tcgtcacctt cctcgccatg ctggagctga tgaagctgaa ccatctccag gtggagcagg
     2761 agggcatgct cggcgagatc atcgtgctgc gccccgcgca gtgaggcggg ggcggttttg
     2821 cgaaagaaaa agtttttttc cacggggtgg catccctgtc agcgcgcgtt tgacacgggc
     2881 ggaggcaggg ggagagtgcc ggacatggag gagcagccag acatccccga agaagtgaag
     2941 aaagagaaag gcctgtggga gaagacccgc aagagcctca tcgagcgtct caacaactgg
     3001 gaggaccagc gcacctggaa tgagttttac cagacctact ggcggctcat ctacagtgtg
     3061 gccacgaagg ccggtctgac gcgggaggag gcgtttgacg tgatccagga gaccatcatc
     3121 gccatcgccc gccaggtgca gaaggggcag tacgacccgc gcgccggctc cttcaaggcc
     3181 tggctgctcc agatgacgcg ctggcgcgtg ctggatgtct tccgcgcccg caagcgccag
     3241 ccctcgctgg ccgaccaggg caacgccgac agcgaggaga ccagcaacct ggccctggag
     3301 cgcctctcca atgaaaagga caacctgctg gaaaaaatct gggaccgcga atggcgggac
     3361 aacatcaccg ccgccgcgct ggagaaagtg aaggccaagg tctcgcccag gcagtttcaa
     3421 atcttcgact gctacgtcat gaaaggctgg ggggtgaaaa agaccagcga ggccctcggc
     3481 atcaacgccg cccaggtcta tctggcgaag catcgtgtcg gcgccctggt caaaaaggaa
     3541 atccaggcgc tcgagcacac gatgctgtga gccgcggacc tgccgggaga gggggctgaa
     3601 cattcgagat tcgaagccgg aaaccgggct tcgaatttcc accccaagcc ccggcgtcca
     3661 gtcaggctgc gcccgcgccg cgtcactccc ggagaatgac gcgcccgccg cgtatcggag
     3721 tggcgctggc tttctggccc ggccctggat tctcggccag ggtgaagtgg cccgcgccgc
     3781 cgccgcccgc cacgggaacc acgatgccgg ccagcttcac cgcgcgcttg gtctgctgcg
     3841 gggtgccggg gttttcaatc aaggtgaaac cgccgctgat ccggccgctg gcggcggcca
     3901 ccgccacttt caatcccgtg atctgcgtgg tgcccggcgg gccggcgacc gcgccgaggg
     3961 tgacgatgtt tttgtcgctg atggcggcat tgcattggaa ttcgttcgcg cccaggccgc
     4021 cgctgcgcag ttccagcacc attgccggcg ccagtccggt gaggcgttgt ccgggagctg
     4081 ctttgacgaa acgcgcgccc tgcacatcca gcgccagggg gccgaatccg ttggtgtaac
     4141 tgcgcgcggg cttgagctgc gccagcttcc gccagtccat ttccaggccg ggcacggcat
     4201 cccatgcacc gggcaggtca ttgacgcgcg cgccgccctg cacgctgccg gtgccggcgt
     4261 aaaggggcgc gtgcaggtgc agttgcgcag cctccgcgcc ggcgttgcgg cccaggagat
     4321 gcgcggcggt gaaggtggcg ccgtcggcga gcacgccggc ccatttcgcc gagcctgtgg
     4381 cgccaagggt gactacggca aagctgtcgc catccggcgc cagggcgctg tcgaggccgc
     4441 cggggccggt gagggccagg ttgtggagtc cggtgagggt ggttttgttt ttggcgctga
     4501 aggggcactg ccagccgtta agatccgtgc tctccgccgt gccttcctcg tccaggctgc
     4561 cggtcaaggc ggcgaggttg gcaaagagcg gcaccttcaa aatcacacgg ctctggcccg
     4621 tcgcacgctt gaccggcgcg gtcagcgaag cgcccgcgcc gccgggctgc acgtcgagct
     4681 ggccggtgat ggggaacttt ttgccgcgct ccagcagcga gccggtgact ttgccgccgg
     4741 tggtgacggt cagccgcgcg tggccgccg
//
LOCUS       DAOMYJ010000313         4756 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Prosthecobacter sp. isolate AMR_MDS_5629
            NODE_6247_length_4756_cov_14.775154, whole genome shotgun sequence.
ACCESSION   DAOMYJ010000313 DAOMYJ010000000
VERSION     DAOMYJ010000313.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867850
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prosthecobacter sp. (wastewater metagenome)
  ORGANISM  Prosthecobacter sp.
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Verrucomicrobiaceae; Prosthecobacter.
REFERENCE   1  (bases 1 to 4756)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4756)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.30
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:34:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,742
            CDSs (total)                      :: 4,684
            Genes (coding)                    :: 4,676
            CDSs (with protein)               :: 4,676
            Genes (RNA)                       :: 58
            tRNAs                             :: 55
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4756
                     /organism="Prosthecobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6247_length_4756_cov_14.775154"
                     /isolate="AMR_MDS_5629"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1965333"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..248)
                     /locus_tag="PLP58_20795"
     CDS             complement(<1..248)
                     /locus_tag="PLP58_20795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ11031.1"
                     /translation="MNIPRVPAFPLLAVFSVLALSQCMTPKYREVRRQAPGGGWYVQR
                     VPINPGEKIERVKRPKPPKAPRPPKEEKPAPLVEDKPAP"
     gene            complement(355..1146)
                     /locus_tag="PLP58_20800"
     CDS             complement(355..1146)
                     /locus_tag="PLP58_20800"
                     /inference="COORDINATES: protein motif:HMM:TIGR00010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HRJ11032.1"
                     /translation="MFFDTHTHLGSRQFDADLPVVLARAAAAGVTRMLAPAVDLENTR
                     KLLALSADLPQVRVAAGIHPCDADSVSGEAWLDELRALARHEKAAAIGEIGLDYFHAP
                     PEGWTVDVWRAHQARCLRMQIELAVELGLNVVLHNRESWDDLVAQITPFHGRLRAVFH
                     CFTGTLEQARPLLEAGHLISFTGIVTFKNPGPAGETARGVPDGGYMLETDAPYLAPVP
                     YRGKRCEPAYVADTARRVAEMRGQSVEEVARATTETALAFFRGFE"
     gene            1255..2871
                     /locus_tag="PLP58_20805"
     CDS             1255..2871
                     /locus_tag="PLP58_20805"
                     /EC_number="3.1.6.-"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arylsulfatase"
                     /protein_id="HRJ11033.1"
                     /translation="MKRLLLLLLAALALPVFGRTNFVVILADDMGWSDAGCYGGEIAT
                     PNLDALARDGLRFTQFYNTARCWPTRSALLTGYYAQQIHRDELPGIGGGGRGTRQPWA
                     RLLPDFLRPAGYRCYHSGKWHIDGLVLEGGFHRSLDIRNQGDFFSTAGNFLDDKPMTP
                     VRTGPAAYSTLINADHAIECLKDHAASHAGSPFFHYIAFIAPHFPLHALPEDIARYRD
                     TYLQGWEKMRAARHARQIETGLHNTHLSALEPEVGPPYHFPDALEKLGPGEVNRPLPW
                     DSLTGEQRRFQAAKMAIHAAMVDRMDREIGRILEQIKAMGAWDDTLILFASDNGASAE
                     IMVRHGGHDPRADPGSSATYLCLGPGFSSACNTPFRRHKTWVHEGGISTPLIAHWPRG
                     IRDKGALRSTPGHVIDIVPTLLEIAGVEKPAQWQGQPIPPAPGRSLLPVFAKDTPLQR
                     DSLWWLHEGNRALRAGDWKLTAAKDQPWELYDLSTDRAEQHDLAARMPEKVRELEAAW
                     QARTDEFTTLAQKTLHLQPPPGAGKKKKAR"
     gene            complement(2951..3433)
                     /locus_tag="PLP58_20810"
     CDS             complement(2951..3433)
                     /locus_tag="PLP58_20810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ11034.1"
                     /translation="MLKVSDFTHKVADFTVKVSDFTVKVTDFTLKVSDFTVKVSDFTL
                     KVADFTVKVSDFTLKVSDFTVKVTDFWPSAVISSKPFLGQPPREASLNPLIMSEWRLF
                     IIFSLRKNAGAFRGMPKTLGMGRWQPACADRRPVCEGGAPSWTRHALARGRVPKAGAN
                     "
     gene            3680..3817
                     /locus_tag="PLP58_20815"
     CDS             3680..3817
                     /locus_tag="PLP58_20815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ11035.1"
                     /translation="MKRDSDLDHTLTGLTPGSTVAAYVIAANPGGEAAPSPTVTKVVG
                     A"
     gene            complement(3997..4593)
                     /locus_tag="PLP58_20820"
     CDS             complement(3997..4593)
                     /locus_tag="PLP58_20820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ11036.1"
                     /translation="MKQALPTSQSRMLNRIGTLLLIASSLALNINAQDLPSEVKALKE
                     SYEREMERVLSPIQSKYIAALEQLQRNYTRAGKLNEAVFVAKEIELAKEWESLPLNQL
                     RGNAKMQMSRDDFRNWLAKKTFTFRGVQLVTLQFEEKHVSWIVGNQPQKYEYKVTGDR
                     RISIMGAQEFKLEFSKDLTSGTFESNAGKYNLTITDRQ"
BASE COUNT          892 a         1720 c         1334 g          810 t
ORIGIN      
        1 ggggctggct tgtcttccac caagggagcg ggcttttcct ccttgggcgg ccttggggcc
       61 ttgggcggtt tgggccgctt cactctctcg atcttctcac cggggttgat cgggacgcgc
      121 tgcacatacc agccgccgcc gggagcctgc ctgcgcacct cgcggtactt cggcgtcatg
      181 cattgggaga gggccaggac cgagaagacg gccagcaggg gaaaggcagg aacccggggg
      241 atgttcatgg ggagtgactt gttgttcgag ccgccttaaa gcacagcgtg ccggaaacgc
      301 catcagaaat ccgcgcgcca gtgccggcgc cgggcacagg tccgcctggc cgcgtcattc
      361 gaagccgcgg aaaaaagcca gcgccgtctc cgtcgtcgcg cgcgccacct cctccacgct
      421 ctggccgcgc atttccgcca cacggcgcgc cgtgtccgcg acataagcgg gctcgcagcg
      481 tttgccgcga tacggcaccg gcgccaggta aggcgcgtcc gtctccagca tgtagccgcc
      541 gtccggcacg ccgcgcgcgg tttccccagc gggaccggga tttttaaagg tcacgatgcc
      601 cgtgaaggaa atcaggtgcc ccgcctccag caaaggccgc gcctgctcca gggtgccggt
      661 gaagcagtga aacacggcgc gcagcctgcc atgaaaaggt gtgatctgcg ccaccagatc
      721 atcccagctc tcgcggttgt gcagcaccac gttgagaccg agctccaccg ccagctcgat
      781 ctgcatccgc aggcagcgcg cctgatgcgc gcgccagaca tccaccgtcc agccctccgg
      841 cggcgcgtgg aagtaatcca gcccgatctc gccgatggcg gccgcctttt catgacgcgc
      901 cagcgcgcgc aattcgtcca gccatgcctc gccgctgaca ctgtccgcat cacagggatg
      961 gatgcccgcc gccacgcgca cctgcggcag gtccgccgag agcgccagca gcttgcgcgt
     1021 gttctccagg tccaccgccg gggccagcat ccgcgtcaca ccggcggccg ccgcgcgcgc
     1081 cagcaccacc ggcaggtccg cgtcaaactg ccggctgccc aggtgcgtgt gcgtgtcaaa
     1141 aaacatgccg ctcctaaagc cggggactca cgcgctggca acggccaatg cgcggggaac
     1201 ggcggcggcg tccccgcgca agagccgcgc ggcgcccgcg ttcccacgcc ctccatgaaa
     1261 cgcctcctcc tgctgctgct cgccgccctc gccctgcccg tgtttggcag gaccaacttc
     1321 gtcgtcatcc tggccgatga catgggctgg tcagacgccg gctgttacgg cggcgagatc
     1381 gccacgccga acctcgacgc cctggcgcgg gacgggctgc gcttcaccca gttttacaac
     1441 accgcccgct gctggcccac ccgctccgcc ctgctcaccg gctactacgc ccagcagatc
     1501 caccgcgacg agctccccgg catcggcggc ggcgggcgtg gcacccgcca gccctgggcg
     1561 cgcctgctgc cggacttcct ccgccccgcc ggctaccgct gctaccacag cggcaagtgg
     1621 cacattgacg gcctcgtcct cgaaggcggc ttccaccgct ccctcgacat ccgcaaccag
     1681 ggcgacttct tcagcaccgc cggcaacttc ctcgacgaca agcctatgac ccccgtgcgc
     1741 accggccccg ccgcctactc caccctcatc aacgccgacc acgccatcga gtgcctcaaa
     1801 gaccacgccg ccagccacgc aggcagcccc tttttccact acatcgcctt catcgccccc
     1861 cacttccccc tccacgccct gcccgaggac atcgcccgct accgcgacac ctacctccaa
     1921 ggctgggaaa aaatgcgcgc cgcccgccat gcccgccaga tcgaaacggg cctccacaac
     1981 acccacctct ccgccctgga gccggaagtc ggccccccct accacttccc cgacgccctc
     2041 gaaaaactcg gccccggcga ggtcaaccgc cccctcccct gggacagcct caccggcgag
     2101 cagcgccgtt tccaggccgc gaagatggcc atccacgccg ccatggtgga ccgcatggac
     2161 cgcgagatcg gccgcatttt ggagcagatc aaagccatgg gcgcctggga cgacaccctc
     2221 atcctcttcg cctccgacaa cggcgccagc gccgagatca tggtccgcca cggcgggcac
     2281 gacccccgcg ccgaccccgg cagctccgcc acctacctct gcctcggccc cggcttctcc
     2341 agcgcctgca acaccccctt ccgccgccac aagacctggg tccacgaggg cggcatctcc
     2401 acccccctca tcgcccactg gccgcgcggc atccgcgaca aaggcgcgct gcgctccacc
     2461 cccggccacg tcatagacat cgtccccacc ctcctggaga tcgccggagt cgaaaagccc
     2521 gcccagtggc agggccagcc catccccccg gcccccggca gaagcctcct gcccgtcttc
     2581 gccaaagaca cccccctcca gcgcgactcc ctctggtggc tccacgaggg caaccgcgcc
     2641 ctccgcgccg gcgactggaa gctcaccgcc gccaaagacc agccctggga actctacgac
     2701 ctctccaccg accgcgccga gcagcacgac ctcgccgccc gcatgcccga aaaagtccgc
     2761 gagcttgaag ccgcctggca ggccaggacc gacgagttca ccaccctggc gcaaaagacc
     2821 ctccacctcc agccgccccc cggcgctgga aaaaagaaaa aagccaggtg agcctctccc
     2881 cgcgcctcca aaccgggaag actcacgtct tcctctacgt ccgccgccgc gtgtcccgtg
     2941 gaggcaaacg tcagttcgcc cccgccttgg gaactcgccc ccgcgccagc gcatggcgtg
     3001 tccaggaggg cgcacccccc tcacagacgg gacgcctgtc cgcgcaggca ggctgccagc
     3061 gccccatgcc cagagttttc ggcattcccc taaaagcacc agcattcttt cttagactaa
     3121 agataatgaa taaacgccat tcactcatta tcagcggatt gagactggcc tcccttggag
     3181 gctgaccaag aaaaggcttg gaagagatca ctgcggaagg ccaaaagtca gtgactttga
     3241 ccgtaaagtc actgacttta agcgtgaagt cactgacttt gaccgtgaag tcagcgactt
     3301 taagcgtgaa gtcactgact ttgaccgtga agtcactgac tttgagcgtg aagtcagtga
     3361 ctttgaccgt aaagtcactg actttgaccg tgaagtcagc gactttatgc gtgaagtcac
     3421 tgactttgag catgaagtca gtgacattga acgcgagatc accagtattt ggcggccaac
     3481 cccaggctcc gcgctcatgt gctgcgactc gtcatcaggt tccgtggaaa gcgcgctcat
     3541 gttcaagccc gaaacgccgc agtctggcgg ctgcgcctac gcggggaggg cacagtcaca
     3601 gatgcccggc agcttgatgc aacttgatac gagatgccgg gcttctgccc ctggagcctg
     3661 tcagaaagtt ttccgcttct tgaagcggga cagcgacctg gaccacaccc tcaccggcct
     3721 cacgcccggc agcaccgtcg ccgcctacgt catcgccgcc aaccccggcg gcgaggccgc
     3781 gccctcgccc accgtgacga aggtggtcgg agcctgagaa gcccccgccg cccccgtcaa
     3841 cgcgtcacca cgcccccacc cgcgtggtga cgccgcccgc cgccactggg aggcggctgg
     3901 aacgaggcgg atttcagcaa gatgcctctc tgtcagatga ggcgcctgca agcagctgat
     3961 ccttaccact cctgacgacc aaaatccgcg cgaagattac tggcgatccg tgatcgtgag
     4021 attgtatttg cccgcgttgg attcaaacgt tcctgatgtc aggtctttcg agaattccaa
     4081 tttaaattct tgagccccca tgatggaaat gcggcggtcg cctgtaactt tgtattcata
     4141 cttctgaggc tgatttccaa caatccaaga aacgtgcttt tcctcgaact gtagcgtcac
     4201 gagctggacc cctctgaatg tgaacgtctt tttggccagc caatttctaa agtcatcacg
     4261 gctcatctgc attttcgcat tgccacgcag ctggttgagc ggaaggcttt cccactcttt
     4321 tgcaagttca atctccttgg caacaaatac ggcctcattc agcttcccag cacgtgtata
     4381 attgcgttga agctgctcaa gggcggcgat gtatttgctt tgaatagggg acaagacccg
     4441 ctccatttcg cgttcgtagc tctctttcag agccttgact tcggatggca ggtcttgcgc
     4501 atttatgttc aaagccagag aagatgcgat aagaagcaga gttccgatgc ggttaagcat
     4561 gcgcgactga gatgttggca gagcttgttt cataggagtg attcaataga tggttgaagc
     4621 ttttcaacgg gcagtgtgcc catttggaaa gccggggtca aggcgggatt gatgccggtc
     4681 tgcctgattc agccggggag ccggtccagg gcccgaagca gggcggggcg ggcggcggag
     4741 aggcctttgt actccg
//
LOCUS       DAOMYJ010000314         4746 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Prosthecobacter sp. isolate AMR_MDS_5629
            NODE_6267_length_4746_cov_16.579621, whole genome shotgun sequence.
ACCESSION   DAOMYJ010000314 DAOMYJ010000000
VERSION     DAOMYJ010000314.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867850
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prosthecobacter sp. (wastewater metagenome)
  ORGANISM  Prosthecobacter sp.
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Verrucomicrobiaceae; Prosthecobacter.
REFERENCE   1  (bases 1 to 4746)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4746)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.30
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:34:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,742
            CDSs (total)                      :: 4,684
            Genes (coding)                    :: 4,676
            CDSs (with protein)               :: 4,676
            Genes (RNA)                       :: 58
            tRNAs                             :: 55
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4746
                     /organism="Prosthecobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6267_length_4746_cov_16.579621"
                     /isolate="AMR_MDS_5629"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1965333"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            383..559
                     /locus_tag="PLP58_20825"
     CDS             383..559
                     /locus_tag="PLP58_20825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ11037.1"
                     /translation="MSDRMQQIIQTKDAERRRLRELPWLEKLDMLTQAPPAPRLPANP
                     AETRCPRIGLSRVR"
     gene            complement(916..2193)
                     /locus_tag="PLP58_20830"
     CDS             complement(916..2193)
                     /locus_tag="PLP58_20830"
                     /inference="COORDINATES: protein motif:HMM:NF014717.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRJ11038.1"
                     /translation="MLKSASLFLALRHLRPRRSFVSVITIISVLGVAVGVLMMIVVRA
                     VMLGFEVDFRDTLMGSEPHVLISRAGSGPAWQDALAGVRARPETVSAAPYAGGMLYMA
                     AGDYQTGGPALALPPAQALPVLEKLRRHLLAGSLDLKEGTIVLSDHHAGQLGVDVGGE
                     ISVYAGQNVNEAVRQYSRANEEDAAENKKAILDAIRLRPRKLAVAGILRAETGGYTAW
                     LGLETGKDIFQPAGGVTGIAIELANPERAVEFQAAAAPELPGWGFTLWTDAGEARLAA
                     MRNEQTMMQFVLSIIALVAAFSVMNTTITVTTQKRKEIGVLAALGAPRGQVVRVFVLQ
                     AAIVGVLGTGFGVTGALLVLWLREDIRALLAALTGGQIHAVEGVFLSSIPAFIQPVDV
                     ALTCLISLVLCLLAGLIPAWAAARVEPAEALRD"
     gene            complement(2246..2677)
                     /locus_tag="PLP58_20835"
     CDS             complement(2246..2677)
                     /locus_tag="PLP58_20835"
                     /inference="COORDINATES: protein motif:HMM:NF019575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HRJ11039.1"
                     /translation="MKFENSSRRTSLGFSLIELLTAIAILGLIAGIGIPVFGGNADHA
                     RHARDQRNAQTISSLYVTAQAAGVDFSTGIQEVAAIVSRLEQGVTVGKGILKGRTYML
                     PNLGTEGFSGAVQFLKLENGMLVYQANGAAVAGEHRAGLAE"
     gene            complement(2831..3643)
                     /gene="murI"
                     /locus_tag="PLP58_20840"
     CDS             complement(2831..3643)
                     /gene="murI"
                     /locus_tag="PLP58_20840"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009960018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008881 - glutamate racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HRJ11040.1"
                     /translation="MPAKDLRSAPLGVFDSGVGGLTVVRALRELLPGESIIYLGDTAR
                     VPYGSKSPDTIRRYAVEDTQFLTRLGVKAVVAACNTATAHALQLLQSTFRVPVIGVLE
                     PGVEATLADPACQRVGIIGTEGTIRSSAYQYSLAMRRPDLRIHAAATPLLVPFIEEGW
                     TEHPALKAVLRDYLKPLLDKGVDTLVLACTHYPLLIPVLKKMLGGKIRLVDSASTCAA
                     HVRSLLDSHDLRRAAKSKPSLEIYLTDLSEKAEVMVRRFLGDDAGKVKKAVL"
     gene            complement(3702..>4746)
                     /locus_tag="PLP58_20845"
     CDS             complement(3702..>4746)
                     /locus_tag="PLP58_20845"
                     /inference="COORDINATES: protein motif:HMM:NF012337.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="beta-ketoacyl synthase N-terminal-like
                     domain-containing protein"
                     /protein_id="HRJ11041.1"
                     /translation="QTDLRPFSRCYRRPLHRHLQDGLLAGRIADRAPLKGRRHAAASN
                     LALHVARRAVARAGWTPEQTREAWLFAASSRGNAAELLGENTWRRPNRRFSASNTLHS
                     EIAAAVSIGLAIRGPWQMLANGCAAGLDALGMAWMAMRAGMTRRALVVAADLPLLPAL
                     LRDFEDTRLLARDEINDPLSPLTNGFHPGEAAAALTLELGDGGLIVEDYRANSDAHDS
                     LVVPEDGGPLAELLHGMGRPDFLCLHATGTPNHAVAEVSALRAAYGEKVPPLLLMKPF
                     TGHTLGASGLLDAALLAGAIQADISPGNLPGLHCPGGLALADALRLPEGGGVLKIASG
                     MGGHNAAVMLRRV"
BASE COUNT          849 a         1462 c         1671 g          764 t
ORIGIN      
        1 gttcaaaccg aaacgggagg gaaacgggga aacggggtca gtgtgcaaaa ttctggggaa
       61 acggggtcag tgtgcaaaat tctgacaaac gcttcatttc tcgcgaggct gcggagcctg
      121 gatcgagccc cgaaatcccc cgccagccgt tccctgggcg tcccggcgac tgctaggacg
      181 cgatgcccga cgaggatgcc atcgcgtcgg acaacaacca cgaaagactc ggaatacacg
      241 gaacgcagaa ccccaaagtc cagcctcttc ccacagattg gctgcgcctg tcttgcggct
      301 cacggaggcc agcacattgg tttcggaaga atctgcgggc ctccttgaat ctgctggaag
      361 ttcgaggatc gtttctcatt ccatgagcga ccggatgcag cagatcatcc aaaccaagga
      421 cgcggaacgc cgccgtctgc gggaactgcc gtggctggag aagctggaca tgctcaccca
      481 agctcctcca gcgccacgcc tgcctgcaaa ccctgcggaa acccgctgcc cgcgcattgg
      541 cctgagtcgt gttcgttgag ccggcgggac gggagcctga atcgcccccg taagccccct
      601 ccagccgttt cctggggtgt cccggcggct ccaactacct tggccgtgaa gccccgcccg
      661 cgatccagtg gtgtcgcgag aggctgcctt tcccaacacc cgctcggcgg cccctccgaa
      721 cggcacaagc acggaaccca aaccttcgga aggctggcaa aaaacacctg tcgtttttta
      781 tttccgaaag ctcggtaaag gaggaagacc tgcttttttg cagacagcgg aaggatgagg
      841 caggcatcgc cactcgctca cggccaagcc gccagtgaac tgtggacagc gaatcgcggt
      901 ggagcggttc acgagtcaat cccgcagcgc ctcggcaggc tcgacgcgtg cggcggccca
      961 tgcggggatg agaccggcga gcaggcagag gaccagggag atgaggcagg tgagggcgac
     1021 atccactggc tggatgaagg cggggatgct ggagagaaaa acgccctcca cggcgtggat
     1081 ctggccgccg gtgagggcgg cgagcagcgc gcggatgtcc tcccgcagcc agagcacaag
     1141 cagggcgccg gtgacgccga aaccggtgcc gagcacaccg acgatggcgg cctggagcac
     1201 gaaaacgcgc accacctgac cgcgcggggc gccgagggcg gcgagcacgc cgatctcctt
     1261 gcgcttctga gtggtgacgg tgatggtggt gttcatgacg gagaaggcgg ccaccagggc
     1321 gatgatggat agcacgaact gcatcatggt ctgctcgttg cgcatggcgg ccaggcgcgc
     1381 ctcgcccgcg tcggtccaga gggtgaagcc ccacccaggc agctccggcg cggcggcggc
     1441 ctggaattcc acggcgcgct ccgggttcgc cagctcgatg gcgataccgg tgacgccgcc
     1501 ggcgggctgg aagatgtctt ttcccgtctc cagccccagc caggcggtgt agccgccggt
     1561 ctcggcgcgc aggatgccgg ccacggccag cttgcgcggg cgcaggcgga tggcgtctaa
     1621 aatggccttt ttgttttcgg ccgcatcctc ctcgttggcg cggctgtatt ggcggacggc
     1681 ttcgttcacg ttctgcccgg catagacgct gatctcgccg ccgacatcca cgcccaactg
     1741 cccggcatgg tggtcggaaa gaacgatggt cccctctttc aaatccaggc tgccggccag
     1801 caggtggcgg cgcaactttt ccaaaacggg cagggcctgc gcgggcggca gcgccagggc
     1861 cgggccgccg gtctggtaat cccccgccgc catgtagagc atgccgccgg cgtagggcgc
     1921 ggcggagacg gtctccgggc gggcgcggac accggccagc gcgtcctgcc aagcggggcc
     1981 ggaaccggcg cgggagatga ggacgtgggg ttcggacccc atgagggtgt cgcgaaagtc
     2041 cacctcgaag ccgagcatga cggcgcgcac gacgatcatc atgagcacgc cgacggccac
     2101 accgagcacg gagatgatgg tgatgaccga gacgaaagac cggcgcgggc gcagatggcg
     2161 gagggccaga aaaagggagg cggatttcag catgggcggc gcattgtccg gcaggagcgc
     2221 cgcacatgcc aggggcaaaa cgcgctcact ccgccaggcc ggcgcggtgc tctcccgcaa
     2281 cggcggcgcc attggcctga tagacgagca tgccgttttc cagcttgagg aactggaccg
     2341 cgccgctgaa cccctcggtg cccagattcg gcagcatgta ggtgcggcct ttgaggatgc
     2401 ctttgcccac agtcacgccc tgctccagcc ggctgacgat ggcagcgact tcctggatgc
     2461 cggtggagaa atccacaccg gcggcctggg cggtgacata gagggagctg atggtctgag
     2521 cgttgcgctg gtcgcgcgca tggcgggcgt ggtccgcatt gccgccgaag acaggaatgc
     2581 cgatgccggc gatcaaaccg agaatggcga tggcggtgag cagctcgatg aggctgaagc
     2641 ccagagaggt gcggcggctg gaattctcaa atttcatatt cgtggctttt atttgtctaa
     2701 attatgataa tttaaagcgt gatggcaaca ctttattctg gcaattatat aaaactcgct
     2761 cgattgattt tcaaaaatga gaattgccac cgaaccatgt cgttggcgcg tgcctcaagg
     2821 gcctgcggga tcaaagcacc gcctttttca ccttccctgc atcgtcgccc aggaagcggc
     2881 gcaccatgac ctcggccttc tcggagaggt cggtgaggta gatttccaga gagggcttgg
     2941 atttggcggc gcggcggagg tcgtgggagt ccagcagact gcgcacatgc gctgcgcagg
     3001 tgctggcgct gtccacgagg cggattttgc cgccgagcat ttttttcagc acagggatga
     3061 gcagcggata atgggtgcag gcgagcacga gcgtgtccac gcctttgtcc agcagcggtt
     3121 tgaggtagtc cctgagcacg gctttgagcg ccggatgctc cgtccagcct tcctcgatga
     3181 agggcacgag cagcggcgtg gcggcggcgt ggatgcgcag gtcggggcgg cgcatggcca
     3241 gggagtattg gtaggcgctg ctgcggatgg tgccctcagt gccgatgatg ccgacgcgct
     3301 ggcaggcggg atcggccagg gtggcctcga ctccaggctc cagcacgccg atgacgggca
     3361 ctcggaaggt ggactgcaaa agctgcaggg catgggcggt ggcggtgttg caggcggcga
     3421 cgacggcctt caccccgagc cgggtgagga attgcgtgtc ctcgacggcg taacggcgga
     3481 tggtgtcggg ggatttcgag ccataaggca cgcgcgcggt gtcgccaaga tagatgatgg
     3541 actcgccggg cagcagctcg cgcagagcgc gcaccacggt cagtccgccg acgccggagt
     3601 cgaaaacacc gaggggggcg gagcggaggt ctttggcggg catgggcgcg caagatggcc
     3661 gtgtccacgc ggcgggcaag cgcgcgcttt gctgccgcga atcaaacgcg ccggagcatg
     3721 accgcggcat tgtgcccgcc catgccggag gcgattttta aaacgccacc gccctccggc
     3781 aggcgcagcg cgtccgccag cgcgagtccg ccggggcagt gaaggccggg caggtttccc
     3841 ggtgaaatat ccgcctggat cgcgccggcc agcagcgcgg catccagcag gccgctggcg
     3901 ccgagggtgt gtcccgtgaa gggtttcatc agcagcaggg gcggcacttt ttcaccatag
     3961 gcggcgcgca gggcgctcac ctccgccacg gcgtggttcg gggtgccggt ggcgtgtagg
     4021 cagagaaagt ccggtctgcc catgccgtgc agtagctccg ccagagggcc gccatcctcg
     4081 ggcacgacga gcgagtcgtg ggcgtcgctg ttggcgcggt agtcctcgac gatcaaaccg
     4141 ccgtcgccaa gctcgagcgt cagcgcggcg gcggcctcgc cggggtggaa gccgttggtc
     4201 agcggtgaaa gagggtcatt gatctcatca cgcgccagca ggcgcgtgtc ctcgaagtcg
     4261 cgcagcagcg ccggcagcag gggcaggtcc gccgcgacga cgagggcgcg gcgggtcatg
     4321 cccgcgcgca ttgccatcca ggccatgccg agggcgtcga gtccggcggc gcagccgttg
     4381 gcgagcatct gccagggtcc gcgaatggcc agcccgatgc tgacggcggc ggcgatctcg
     4441 ctgtgcaggg tgttgctggc gctgaatctc cggttgggcc tgcgccaggt gttttcgccg
     4501 agcagttcgg cggcgttgcc ccggctgctg gcggcgaaaa gccatgcctc gcgtgtctgc
     4561 tccggcgtcc agcccgcgcg cgccaccgcc ctccgcgcca catgcagggc cagattcgac
     4621 gccgccgcgt gacgcctgcc tttgagcggc gcgcggtcgg cgatgcggcc ggccagcagg
     4681 ccgtcttgca ggtgacgatg cagcggccgt ctatagcatc gcgaaaacgg gcgcaagtct
     4741 gtttgc
//
LOCUS       DAOMYJ010000315         4744 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Prosthecobacter sp. isolate AMR_MDS_5629
            NODE_6272_length_4744_cov_17.362764, whole genome shotgun sequence.
ACCESSION   DAOMYJ010000315 DAOMYJ010000000
VERSION     DAOMYJ010000315.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867850
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prosthecobacter sp. (wastewater metagenome)
  ORGANISM  Prosthecobacter sp.
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Verrucomicrobiaceae; Prosthecobacter.
REFERENCE   1  (bases 1 to 4744)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4744)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.30
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:34:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,742
            CDSs (total)                      :: 4,684
            Genes (coding)                    :: 4,676
            CDSs (with protein)               :: 4,676
            Genes (RNA)                       :: 58
            tRNAs                             :: 55
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4744
                     /organism="Prosthecobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6272_length_4744_cov_17.362764"
                     /isolate="AMR_MDS_5629"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1965333"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1625
                     /locus_tag="PLP58_20850"
     CDS             <1..1625
                     /locus_tag="PLP58_20850"
                     /inference="COORDINATES: protein motif:HMM:NF012337.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="beta-ketoacyl synthase N-terminal-like
                     domain-containing protein"
                     /protein_id="HRJ11042.1"
                     /translation="GAAVMLLESADAAAERGADALAVAAGYATATDLHHLTQPDPEGR
                     AAIRTMTGACVMAGLRPDEIHYLNSHGTGTPYNDVAEASAIRSWAGGAAAGLAVSSTK
                     SAMGHLLGGAGAVEAVVCLMAMRGGWLPGSLNVREVDECAGFDLVRAFRQARVRAAMT
                     NSFGFGGTNATLVFSAESPSSGAAPALPVDLRILGGGAVSSAGWTAADLKKAVLENAA
                     PPLSDCPRSIGTRDWECPARRVPAMPPDRIPKSPRLRRASPITKFAMAAALEALEQAG
                     FPQGAGAGRLGIVMAVFNGCVQFSGRFYHEVIETPALASPLIFPETVYNAPASHIAAA
                     LSADGPVSTLVGESSAIADAVTLARLWLEQGLVDHCLVIGAEEADWLGAEAASYYHPQ
                     LPASEGAGALLLGAAHEARADGTPAPHAPSLRVLGTAAFLGENERAAVLEGLALALAA
                     PAPPGLLVDGSSGISVLDSPEEAALRSPGLQPARRLHPRAVIGESMGAAGAFQLVLAA
                     ALAAEEKRVDVLMPGTLCGAHALRVEIDGNAG"
     gene            complement(1645..2907)
                     /locus_tag="PLP58_20855"
     CDS             complement(1645..2907)
                     /locus_tag="PLP58_20855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ11043.1"
                     /translation="MFLPFLAAAALQAEDLRWFKGNTHTHSLWSDGNDFPETIAAWYK
                     DQGYDFLALSDHNTLQKGEKWMTEEAIEKRRVTLGRKVIEKCRDRFGPGWVETRMNAE
                     GKLEVRLKTLEDFRSKLEAPGKFILVQAEEVTASFNKAPIHINAINLENVIEPLKDVV
                     SIRETMRKNLQAIAAEAARSGRPMIAHINHPNFRWALTAEDLAHVIEDKYFEVYNGHP
                     SCYNEGEEGRADTHLGRVWDIANTIRLAELKHPPLYGMATDDSHHYHGGANTPGRGWV
                     MVRAAKLDASVLTEAMQRGDFYATTGILLDDVSFDKVTRRLSLKIQGKPGVTYTTEFR
                     GTLKNYDRAVKEVPTPEGDNHPVRLQHGADVGKLLASSTSLEPSHQLTGDELYVRAVI
                     RSDEEMKNPITPGEKQQAWTQPVGWMAE"
     gene            2961..>4744
                     /locus_tag="PLP58_20860"
     CDS             2961..>4744
                     /locus_tag="PLP58_20860"
                     /inference="COORDINATES: protein motif:HMM:NF024640.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prenyltransferase/squalene oxidase
                     repeat-containing protein"
                     /protein_id="HRJ11044.1"
                     /translation="MIESLETALANTRAHLLSLRSAEGCWKGELSSSALSTATAIVAL
                     HGVDAQKHAPLIAAGARWLVASQNDDGGWGDTTLSKSNLSTTLLCWSALHLDDEDDNG
                     GRGHGDRESRLRAGAWITTRTGSLAPEAIARAVIARYGKDKTFSVPILMLCAICGTLG
                     ARPWLHVLPLPFELAALPRSWFGAIGLPVVSYALPALIAIGHARWRNAPPAWWNPLRW
                     LRAMLWPRVRPMLKALQPSTGGYLEATPLTSFVTMALAAAGEKNHPCVPEAVAFLTRS
                     MRADGSWPIDTNLATWGTTLAVKALGEIEPATADWLLSQQYLEVHPFTNAAPGGWAWT
                     DLPGGVPDADDTSGALIALKLAGEGNSKFEVRNSRPKEPCPRSAIRNPQSAIAWLLNL
                     QNRDGGIPTFCRGWGALPFDRSTPEITAHALLAWWLWDVEELRPRVRDATARALDYLA
                     KTRRADGSWAPLWFGNEHVHEEENPVYGTAMVVGCLSAQPALAEQARVLIKGGAAFLL
                     KAQREDGGWGGAGDAPASIEETAVAVNALASQGGHHRDAVQRGVSWLVSATQGGAHFP
                     PSPIGLYFARLWYHERLYPVVWTLQALRA"
BASE COUNT          710 a         1536 c         1623 g          875 t
ORIGIN      
        1 aaggcgccgc cgtcatgctt ctggagagcg cggacgcggc ggcggagcgc ggggcggacg
       61 cgctggccgt ggcggcgggt tacgccacag ccaccgacct ccaccatctc acccagcccg
      121 atcccgaagg cagggccgcc atccgcacca tgaccggcgc ctgtgtcatg gccggactca
      181 ggccggatga aatccactac ctcaactccc acggcaccgg cacaccctac aatgatgtgg
      241 ccgaggccag cgctatccgc tcctgggccg ggggcgcggc ggcaggcctc gctgtcagct
      301 ccacaaagtc cgccatgggc catttgctag gcggcgcggg cgcggtggag gccgtggtct
      361 gcctcatggc catgcgcggc ggctggctgc ccggcagtct gaatgtgcgc gaagtggatg
      421 aatgcgcggg ttttgatctc gtgcgcgcat tccgccaggc gcgggtgcgc gctgcaatga
      481 ccaactcctt cggtttcggc ggcaccaatg ccactctcgt tttttccgcc gagtccccct
      541 catcgggtgc cgcgccagcg ctgcccgtcg acttgaggat tttggggggc ggcgcggtct
      601 cgtccgctgg ctggacggcc gccgatctca aaaaagccgt tctggaaaat gccgcgccgc
      661 ctttgagtga ctgcccgcgc agcattggca cgcgcgactg ggagtgtccc gcgcgccgcg
      721 tgcccgccat gccgccggac cgcattccaa agtcgccccg tttgcgccgt gccagtccga
      781 ttaccaaatt cgccatggcc gccgcgcttg aggcgctgga acaggcgggt tttccccagg
      841 gcgcgggtgc ggggcggctc ggcatcgtca tggccgtgtt caacggatgc gtgcagttct
      901 ccggccgctt ttatcatgag gtcatcgaga cccccgcgct ggccagcccg cttattttcc
      961 cggagactgt ctataacgcg cccgcctcgc atatcgccgc cgctctcagc gcggacgggc
     1021 cggtgtccac gctcgtcggc gagtcctccg ccatcgcgga tgccgtcaca ctcgcccggc
     1081 tgtggctgga gcagggactc gtggatcact gcctggtcat cggagcggag gaggccgact
     1141 ggctcggcgc ggaggctgcc agttattatc acccgcaact gccggccagt gagggcgcag
     1201 gcgccttgct gcttggagcc gctcatgaag cgcgcgcgga cgggacgccc gcgccccatg
     1261 ctcccagcct gcgagtcctg gggacagccg cttttctcgg agaaaacgag cgcgccgcag
     1321 tgctcgaggg gctagccctt gcccttgccg cccccgcgcc gccgggcctg ctcgtggacg
     1381 gcagcagcgg catctccgtg ctcgattctc cggaggaggc cgctttgcga tccccgggtt
     1441 tgcagccagc gcggcgcttg catccgcgcg ctgtcatcgg agaaagcatg ggcgccgccg
     1501 gtgcttttca actcgtcctg gcagcggcgc tcgccgcgga ggaaaagcgc gtggatgtgc
     1561 tcatgcccgg caccctctgc ggcgctcacg cattgcgcgt cgaaattgac ggcaatgcgg
     1621 gttgagatag gagcgcggtt caaatcactc ggccatccag cccacaggct gagtccatgc
     1681 ctgctgcttc tcccccggcg tgatcggatt tttcatttcc tcgtcggagc ggatgacagc
     1741 gcggacgtaa agctcgtctc cagtgagctg gtgggagggt tcgaggctgg tgctggaggc
     1801 gagcagtttg cccacgtccg cgccatgttg caggcgcacg ggatggttgt cgccctcggg
     1861 ggtgggcacc tctttcaccg cgcggtcata gtttttcaaa gtgccgcgaa actccgtggt
     1921 gtaagtgact ccgggtttgc cctggatttt caagctgagg cggcgggtga ctttatcaaa
     1981 agaaacgtca tcgagcagga tgccggtggt ggcgtaaaaa tccccgcgct gcatggcctc
     2041 ggtgagcacg ctggcgtcga gcttcgcggc acgcaccatc acccagccgc gccccggcgt
     2101 gttggctccg ccgtggtagt ggtgtgaatc gtcggtggcc atgccgtaaa gtggcgggtg
     2161 ctttaactcg gccagccgga tggtgttggc gatgtcccac acgcggccga gatgcgtgtc
     2221 cgcgcggcct tcctccccct cgttgtagca ggaggggtgg ccgttataga cctcgaagta
     2281 tttgtcctcg atgacatgcg ccaggtcctc cgcggtgagc gcccagcgga agttcggatg
     2341 gttgatgtgg gcgatcatgg gccgtccgct gcgcgcggcc tccgcggcga tggcctggag
     2401 gtttttgcgc atcgtttcgc ggatggagac cacatccttg agcggctcga tgacattttc
     2461 gaggttgatg gcgttgatgt gaatcggagc cttgttgaag gaggcggtga cctcctcggc
     2521 ctgtacgagg atgaacttgc cgggcgcttc gagcttggag cggaagtcct ccagcgtttt
     2581 gagccggact tcgagcttgc cctcggcgtt catgcgggtc tccacccagc cggggccgaa
     2641 gcggtcgcgg catttctcga tgacttttct accgagcgtg acgcggcgtt tttcgatggc
     2701 ctcctcggtc atccattttt cgcctttttg cagggtgttg tggtcggaga gcgcgaggaa
     2761 gtcgtagccc tggtccttgt accatgcggc gatggtctcg gggaagtcgt tgccgtcgct
     2821 ccacagcgag tgcgtgtgcg tgttgccttt gaaccagcgc aagtcttcgg cttggagcgc
     2881 ggcggcggcg aggaagggga ggaacattct catgagaatg gcttgaacgc gctccggcgc
     2941 ggcgaacaat cacgcgcttg atgattgagt ccctcgaaac cgccctcgcg aacacccgcg
     3001 cgcatctttt gtccctgcgc agcgcggagg gctgctggaa gggggagctt tccagcagcg
     3061 cgctgagcac ggccacagcc atcgtggctc tgcacggggt ggacgcgcaa aaacacgccc
     3121 cgctcatcgc ggcgggcgcg cgctggctcg tcgccagtca aaatgacgac ggcggctggg
     3181 gtgacacgac cctcagcaag agcaacctct ccaccacgct gctctgctgg agcgcgctgc
     3241 atctggatga tgaggatgac aacggcgggc gcgggcatgg ggaccgggaa tcccgcctgc
     3301 gcgccggggc ctggatcacc acccgcacgg gcagtctcgc gccggaggcc atcgcgcgcg
     3361 cggtcatcgc ccgttacggg aaggacaaga ccttctccgt gcccatcctc atgctgtgcg
     3421 ccatctgcgg cacgcttggc gcgcggccct ggctgcatgt gctgccgctg ccgttcgagc
     3481 tggcggcgct gccgcggagc tggttcggcg ccatcggcct gccggtggtc agttacgcgc
     3541 tgccggcgct catcgccatc ggccacgcgc gctggaggaa cgctccaccg gcgtggtgga
     3601 accctctgcg ctggctgcgc gcaatgttgt ggccgcgcgt ccggcccatg ctcaaggcgc
     3661 tgcaaccctc cactggcggc tacctggagg ccacaccgct gaccagcttt gtgacgatgg
     3721 cactggcggc ggcgggggag aaaaaccacc cgtgtgtgcc ggaagcggtg gcttttctca
     3781 cccgctccat gcgcgcggat ggaagctggc ccatagacac caacctcgcc acttggggaa
     3841 ccacgctcgc cgtgaaggcg ctgggcgaaa tcgagccggc cacggcggac tggctgctga
     3901 gccagcaata cctggaggtg catcctttca ccaacgccgc gcccggcggc tgggcctgga
     3961 cggaccttcc gggcggtgtg cccgacgcgg atgacacctc gggggcgctc atcgctttga
     4021 aactcgcggg ggagggaaac tcgaagttcg aagttcgaaa ttcgaggccc aaagagccat
     4081 gtccccgttc cgcaatccgc aatccgcaat ccgcaatcgc ctggcttctc aacctgcaaa
     4141 accgcgatgg cggcatcccc actttctgcc gcggctgggg cgcgctgccg tttgaccgca
     4201 gcactccgga gatcaccgcc catgccttgc tggcgtggtg gctttgggat gtggaggaac
     4261 tgcgcccgcg tgtgcgggac gccacggcgc gggcgctgga ttacctggca aaaacgcggc
     4321 gcgcggacgg ctcgtgggcg ccgctctggt tcggcaacga gcatgttcat gaggaggaga
     4381 accccgtcta tggcacggcg atggtggtgg gttgcctcag cgcgcagccg gcgctggcgg
     4441 agcaggcgcg tgtcttgatc aaaggcggcg cggcttttct cctgaaagcg cagcgggagg
     4501 atggcggctg gggcggggca ggggacgcgc ccgccagcat tgaggagaca gcggtggcgg
     4561 tgaacgcgct ggcttcacag ggcgggcatc atcgcgatgc cgtgcagcgc ggtgtttcat
     4621 ggctcgtttc cgccacgcaa ggcggcgcgc atttcccccc cagccccatc ggcctctact
     4681 tcgcgcgctt gtggtaccac gagcggctct atcccgtggt ctggacgctc caggctctgc
     4741 gcgc
//
LOCUS       DAOMYJ010000316         4729 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Prosthecobacter sp. isolate AMR_MDS_5629
            NODE_6301_length_4729_cov_21.012837, whole genome shotgun sequence.
ACCESSION   DAOMYJ010000316 DAOMYJ010000000
VERSION     DAOMYJ010000316.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867850
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prosthecobacter sp. (wastewater metagenome)
  ORGANISM  Prosthecobacter sp.
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Verrucomicrobiaceae; Prosthecobacter.
REFERENCE   1  (bases 1 to 4729)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4729)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.30
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:34:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,742
            CDSs (total)                      :: 4,684
            Genes (coding)                    :: 4,676
            CDSs (with protein)               :: 4,676
            Genes (RNA)                       :: 58
            tRNAs                             :: 55
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4729
                     /organism="Prosthecobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6301_length_4729_cov_21.012837"
                     /isolate="AMR_MDS_5629"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1965333"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..543
                     /locus_tag="PLP58_20865"
     CDS             <1..543
                     /locus_tag="PLP58_20865"
                     /inference="COORDINATES: protein motif:HMM:NF015679.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HRJ11045.1"
                     /translation="SGPCRRSACDLPHEHEERAPKTEEPAPVDDGSFWIGDGVSGKPK
                     IRISLGEQKISYFKGGELVGQSPMSSGREGYKTVVGTFFISEKDEDHKSSLYGNYVDE
                     TGLVLKEDVDVRKDPKPPGAKFDGADMRWFMRIHKAIGMHEGYLPGFPASHGCIRLPS
                     RMAEIFYKATPPGTEVEITH"
     gene            complement(620..853)
                     /locus_tag="PLP58_20870"
     CDS             complement(620..853)
                     /locus_tag="PLP58_20870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009959880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HRJ11046.1"
                     /translation="MADAANKYPQNITGAYYVDDQCIDCDLCRETAPANFTRDDDGGH
                     SYVYKQPETDDERAQCDEAMAGCPVEAIGSDGE"
     gene            986..1813
                     /locus_tag="PLP58_20875"
     CDS             986..1813
                     /locus_tag="PLP58_20875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009959879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chlorite dismutase family protein"
                     /protein_id="HRJ11047.1"
                     /translation="MNQDPPATPLVPQEGWTVQHLFFHIDHGFWAALTPSEKAERAAR
                     MQCTVDAIRAHPKTQLLTFSMVGPKADLGFMLLTPDLHDSDRFSKQLGLALGPDVLTP
                     CYNYLSMTEWTEYSESEDEAAARIQRTENLQPGSEEFEKRLGEWKAHMGKYYQDRLYP
                     NMPDWPVMCFYPMSKRRNVGANWYAQDFAKRRELMGGHAKTGRKYAGKVRQLVTGSTG
                     LDSHEWGVTLFAHDVFQIKSIVYEMRWDEVTTQFGEFGDFFIGIQLPVPALVERALA"
     gene            1839..2621
                     /locus_tag="PLP58_20880"
     CDS             1839..2621
                     /locus_tag="PLP58_20880"
                     /EC_number="2.8.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018968836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1990107 - thiazole synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiazole synthase"
                     /protein_id="HRJ11048.1"
                     /translation="MPNTPLIIAGREFASRLMLGTGKFASLDLMRQAIAASGTEIVTV
                     ALRRADLSGKGDPFANILDFIPENVLLLPNTSGAFTAEEAVRLARLAVAAGMPNWVKL
                     EIHPDPRYLLPDPIETLKAAEILVKEGFTVLPYINADPVLARRLQDAGTATVMPLGSP
                     IGSHQGITTRRQIEIILSQATVPVVVDAGIGAPSHAAEALEMGADAVLVNTAIAIASD
                     PVRMASAFKAAVEAGRAAYEIGLADSTDEASATSPLTGFLYS"
     gene            2630..3700
                     /locus_tag="PLP58_20885"
     CDS             2630..3700
                     /locus_tag="PLP58_20885"
                     /EC_number="2.1.1.172"
                     /EC_number="2.1.1.174"
                     /inference="COORDINATES: protein motif:HMM:NF017026.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0052916 - 23S rRNA
                     (guanine(1835)-N(2))-methyltransferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HRJ11049.1"
                     /translation="MKALEALFHTLDTQPEIPLDGSILFLHALAHPALEAWRGRLTCE
                     QVWKPAALQLQQSGIPSTRTATGTFDTVLLLPERQRDTVLADFAKAHDLLNEGGTLVV
                     ALHNDWGAKRMEQQLANVAGELQTVSKSHCRVFWAKKHPGWKTVQLAQWRQNAAMRRI
                     LDGRFWSMPGLFHWDAIDEGSALLVEHLPRDLRGAVADLGAGWGFLSQHILQHCPDIR
                     SLDMYEADARALECARRNTGLIPVPVRPKLHWHDVTQGLGGAMYDTVVMNPPFHDGRD
                     SNSLLGLKFITSAAQALRPGGELWLVANRHLPYENLMAEAFETHRTTGENKAFKVLHG
                     KNPARVTRMSHARPHRGRKGGP"
     gene            3796..4506
                     /gene="purQ"
                     /locus_tag="PLP58_20890"
     CDS             3796..4506
                     /gene="purQ"
                     /locus_tag="PLP58_20890"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012419588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase I"
                     /protein_id="HRJ11050.1"
                     /translation="MPHALLIKFPGTNCDAETARALEAAGFTAEVLPASQLAPAALER
                     ARLVVFSGGFSYGDYVMSGRIAQLITKHKLGEKLKDFVAGGGHVLGICNGFQILAQLD
                     LLPRGSLIHNESGRFICKWAPLKKNSSTSPFLKALPDDFELPVAHAEGRFVGAPGDAE
                     KYVKDGHAALLYGADVNGSSARIAGMQDDTGRVFGLMPHPERFLSKAHHYDPDWSRAE
                     HGWGYYFFKGAAEAMAAA"
BASE COUNT          866 a         1605 c         1443 g          815 t
ORIGIN      
        1 tcggggcctt gtcgccgctc agcgtgcgac cttccgcacg agcatgaaga gcgcgcgccg
       61 aagaccgagg agcccgcgcc cgtggacgac ggctcctttt ggatcggcga tggcgtctcc
      121 ggcaagccga agatcaggat cagcctcggc gagcaaaaga tcagctattt taaaggcggc
      181 gagctggtgg ggcagtcgcc catgtcctca ggtcgcgagg gttacaagac cgtggtgggc
      241 acctttttca tcagcgagaa ggacgaggac cacaaaagct ccctctacgg caactatgtg
      301 gatgagaccg gcctggtgct gaaggaggat gtggatgtgc gcaaggatcc gaagccgccg
      361 ggcgcgaagt ttgacggcgc ggacatgcgc tggttcatgc gcatccacaa ggccatcggc
      421 atgcacgagg gctacctgcc cggcttcccc gcctcgcacg gctgcatccg cctgccttcg
      481 cgcatggcgg agattttcta caaggccacg ccgcccggaa ccgaggtgga gatcacccac
      541 tgagcggggg aagacccggc caaaaaaaag gcatgaccgg agtcatgcct ttgagtttgg
      601 aaaaaatgaa gcgggcggat cactctccgt cgctgccgat ggcctcgacc gggcagccgg
      661 ccattgcctc gtcgcattgg gcgcgctcgt cgtcggtctc gggctgcttg taaacgtagg
      721 agtggccgcc gtcgtcgtcg cgggtgaagt tggcgggggc tgtctcgcgg cacagatcgc
      781 agtcaatgca ttgatcgtcc acatagtagg cgccggtgat gttctggggg tatttgtttg
      841 ctgcgtcagc catgaagggt gttctcctgt cggggtgaat tgggcggcga tggtaatgcc
      901 gggactccgg gtttcaatca ctttttgcga atccctgctc ccgtggttga gcaggcggca
      961 ttccagagta tggacaaaat tcaccatgaa tcaggatccc cccgcaaccc ccctcgtccc
     1021 ccaggaaggc tggacggtgc agcatctgtt tttccacatt gaccacggct tctgggccgc
     1081 gctcacgccc tcggagaaag ccgagcgcgc cgcccggatg caatgcaccg tggacgccat
     1141 ccgcgcgcac ccgaagacgc agcttctgac cttcagcatg gtgggtccga aggcggacct
     1201 cggcttcatg ctgctgacgc ccgatctgca tgactcggac cgcttttcca agcagctcgg
     1261 ccttgccctc gggccggatg tgctcacgcc ctgctacaac tacctctcga tgaccgagtg
     1321 gaccgagtac agcgagtccg aggatgaggc cgccgcgcgc atccagcgca cggagaacct
     1381 ccagcccggc agcgaggagt ttgaaaaacg cctcggcgag tggaaggcgc acatgggcaa
     1441 gtattaccag gaccgcctct acccgaacat gcccgactgg ccggtgatgt gcttttaccc
     1501 catgagcaag cgccgcaacg tgggagccaa ctggtacgcg caggacttcg ccaaacgccg
     1561 cgagttgatg ggcggccacg ccaagaccgg ccgcaaatac gccggcaagg tgcgccagct
     1621 cgtcaccggc tccaccggac tcgacagcca tgagtggggt gtcacgcttt ttgcccacga
     1681 tgtcttccag atcaaatcca tcgtctatga gatgcgctgg gacgaggtga ccacgcagtt
     1741 cggcgagttt ggcgacttct tcatcggcat ccagctcccc gtccccgccc tcgtcgagcg
     1801 ggcgctggct tgatgacgcg ccgagcatca cctcacccat gcccaacacc cccctcatca
     1861 tcgccggacg cgagtttgcc tcgcggctca tgctcggcac cggcaagttc gcctcgcttg
     1921 atctcatgcg ccaggccatc gccgcctccg gcacggagat cgtcaccgtg gccctgcggc
     1981 gcgcggattt gtcaggcaaa ggcgacccct tcgccaacat cctcgatttc attccggaaa
     2041 atgttcttct gctgccgaac accagcggcg ccttcactgc cgaggaagcc gtgcgcctgg
     2101 cccgcctggc cgtcgccgcc ggaatgccaa actgggtgaa gctcgaaatc caccctgatc
     2161 cgcgctacct gctgcccgac cccatcgaga cgctcaaggc cgccgagatt ctcgtgaagg
     2221 agggtttcac agtgctgccc tacatcaatg ccgacccggt gctcgcgcgc cggctccagg
     2281 acgcgggcac cgccaccgtc atgccgctcg gctcgcccat cggctcgcac cagggcatca
     2341 ccacccggcg gcagatcgag atcattctca gccaggccac cgtgcccgtg gtggtggatg
     2401 ccggtatcgg cgcgcccagc catgccgccg aggccctcga aatgggcgcc gacgccgttt
     2461 tggtgaacac tgccatcgcc atcgcctccg accccgtgcg catggcttcc gcgttcaaag
     2521 ccgccgtcga agccggccgc gccgcctatg aaatcggcct cgcggacagc acggatgagg
     2581 ccagcgccac cagcccgctc accggctttc tctattcctg acgcggcgga tgaaagccct
     2641 cgaagccctt ttccacaccc tcgacacgca gccggagatc cctctcgacg gcagcatcct
     2701 ctttctccac gccctggccc atccggcact ggaggcatgg cgcggcagac tcacctgcga
     2761 gcaggtgtgg aagcccgccg cactccagct ccagcaaagc ggcatcccca gcaccaggac
     2821 cgccaccggc accttcgaca ccgtcctgct cctgcccgaa cgccagcgtg acacggtgct
     2881 cgccgacttc gccaaagccc acgatctttt gaacgaaggc ggcaccctcg tcgtggccct
     2941 gcacaacgat tggggggcaa aacgcatgga gcagcagctc gccaatgtcg cgggtgagtt
     3001 gcagaccgtc tccaaaagtc actgccgcgt cttttgggcg aaaaagcacc ctggttggaa
     3061 gaccgtccag cttgcccagt ggaggcaaaa cgccgccatg cgccgcatcc tcgacggacg
     3121 cttctggtcc atgcccggct tgtttcattg ggatgcgatt gacgaaggct cggccctgct
     3181 cgtcgagcat ctgccgcgcg atctgcgcgg cgccgtggcc gacctcggcg cgggctgggg
     3241 cttcctcagc cagcacatcc tccaacattg cccggacata cgcagcctcg acatgtacga
     3301 ggccgacgcc cgcgccctgg agtgcgcgcg gcggaacacc ggcctcatcc ccgtccccgt
     3361 gcgcccgaag ctccactggc acgatgtcac ccagggactc ggcggcgcca tgtatgacac
     3421 agtcgtcatg aacccgccct tccatgacgg acgggattcg aactccctgc tcggcttgaa
     3481 attcatcacc agcgccgccc aggctctgcg ccccggaggc gagctttggc tcgtcgccaa
     3541 ccgccatctg ccctatgaaa acctcatggc cgaggcattc gagacgcacc gcaccaccgg
     3601 cgaaaacaaa gccttcaaag tcctccacgg caaaaacccc gcccgagtca cccgcatgag
     3661 ccatgcgcgc ccgcaccgcg gccgcaaggg cgggccgtga ggagcgcgag ccttcggcgg
     3721 agcggatgcg ggacaatttg ccggctttca gggaaggcct cttgcatcct gcgcgggcat
     3781 cgccataaaa gccccatgcc ccacgcattg ctcatcaaat ttcccggcac caactgcgat
     3841 gctgaaaccg cccgcgccct ggaggcggct ggcttcaccg ccgaggttct gcccgcctcg
     3901 cagctcgccc ccgcggccct ggagcgggcg cggctcgtgg tcttctccgg cggtttcagc
     3961 tatggcgact atgtcatgag cggccgcatc gcccagttga tcaccaagca caagctgggg
     4021 gagaaattga aagacttcgt ggccggtggg ggccatgtgc tgggcatttg caacggcttc
     4081 caaatcctgg cgcagctcga cctcctgccg cgcggcagcc tgatccacaa cgagagcggg
     4141 cgcttcatct gcaagtgggc gcccctgaaa aaaaacagct ccaccagccc ctttctcaaa
     4201 gccttgccgg acgacttcga gctgccggtg gctcatgcgg aaggccgctt tgtcggcgcg
     4261 ccgggcgacg cggaaaagta cgtcaaggac gggcacgccg cgctgcttta cggagcggat
     4321 gtgaacggct ccagcgcgcg catcgccggg atgcaggatg acaccgggcg cgtcttcggc
     4381 ctgatgccgc atcctgagcg cttcctgtcc aaagcgcacc actacgatcc ggactggagc
     4441 cgcgcggagc acggctgggg ttattacttc ttcaaaggcg ccgccgaggc gatggccgcc
     4501 gcctgaggcg cggaaaaagt ggctggttcg tgaaatccga aattcggttt tagaatctca
     4561 caccttctcc agcttgacgg ggtaggtctg gaaggcgttc cattggcaga cgatgaggtc
     4621 gccgttgtcc aggacgcaga catcgtggcc gtggtcgaag acgcgctcgg cctgcatgag
     4681 gggctggagt ttgccgtcct catagacggg ggcggtgccg ccgggggcg
//
LOCUS       DAOMYJ010000317         4683 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Prosthecobacter sp. isolate AMR_MDS_5629
            NODE_6378_length_4683_cov_17.815903, whole genome shotgun sequence.
ACCESSION   DAOMYJ010000317 DAOMYJ010000000
VERSION     DAOMYJ010000317.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867850
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prosthecobacter sp. (wastewater metagenome)
  ORGANISM  Prosthecobacter sp.
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Verrucomicrobiaceae; Prosthecobacter.
REFERENCE   1  (bases 1 to 4683)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4683)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.30
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:34:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,742
            CDSs (total)                      :: 4,684
            Genes (coding)                    :: 4,676
            CDSs (with protein)               :: 4,676
            Genes (RNA)                       :: 58
            tRNAs                             :: 55
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4683
                     /organism="Prosthecobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6378_length_4683_cov_17.815903"
                     /isolate="AMR_MDS_5629"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1965333"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(309..1676)
                     /locus_tag="PLP58_20895"
     CDS             complement(309..1676)
                     /locus_tag="PLP58_20895"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase"
                     /protein_id="HRJ11051.1"
                     /translation="MKNLRAAFLLSMLAAAALHAAPPNIIFILVDDMGYGDLGCTGAK
                     DIRTPHIDRLAAEGVKFTDFYSNAPVCTPTRVGFMTGRWQQRTGIEFAFGYQVEQWRK
                     VGGEWVPEPDVHGLGLPAGETTIAQRLKTAGYTTGAFGKWHLGHKDEYNPLHRGFDEY
                     FGELLGHADFYRHTYFDGTHALRDGLQPVQMEGYFTDLVNERAARFIRQHGGRGKKPF
                     FLYVPHLAVHAPFQPPDAPRTPMVTKENMLTGSRAIYKAMLERVDHGVGMILAELEAH
                     GITNDTLIVFSSDNGGERWASNAPYFHHKATLWEGGIRVPCLMSWPARLPKGSVCETP
                     AITMDLHATFLLAAGLPLPEDKPLDGMDLLPHALSAEAAAQDRSLCWRMDRANRKMKA
                     IRHGRWKYINDGNTMDLLFDLKDDPGERVNLGYQHPEIVTGLKARLARWEAEMDATEK
                     EIKVR"
     gene            complement(1692..2375)
                     /locus_tag="PLP58_20900"
     CDS             complement(1692..2375)
                     /locus_tag="PLP58_20900"
                     /inference="COORDINATES: protein motif:HMM:NF013718.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HRJ11052.1"
                     /translation="MMDWDLHLLRKINQDWTHPVLDWLMPAVSCIQAWVPLLILLVIA
                     ILWRGGARARLMLLCVGVAVGTGDGIVGNVLKKHTGQVRPRDALEEVVVRDLGKAPQP
                     EFRRLFLPPVQKPGFRKASPDRKSFPSSHTVNMFALATVVALFYRRWGILAFMFAAAV
                     AWSRVYVGAHWPSDVIPSTGIGILLGWGAVRLAGWAAAKLAARHQMTAASLASKKSPH
                     SALATATAD"
     gene            complement(2372..3913)
                     /locus_tag="PLP58_20905"
     CDS             complement(2372..3913)
                     /locus_tag="PLP58_20905"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HRJ11053.1"
                     /translation="MISDSDDTLWTRLFWRGLAAVFAFRLAFMLFFCQQADLLGDEAY
                     YWDWGRQPDWGYFSKPPMIGWLMGITGWITGNAEWGIRLAPLLLGTGTLAAVFSLARD
                     LYGARAACLAALLIVLTPGNAGLNLFFTIDAPLLLAWSLAMLALWRAAEEAKPAWWLV
                     LALALGFGVLSKQMMLVFPALALVFSAVSKDNRPMLARPGFWLAALAGMAFITPVLMW
                     NARHEWVTLEHTRHHFDTESLGMGKWLGRTLEFPAVQALVYTPVTFATLIFAAWLSLK
                     NWRALGSRELYLLVFSVLPLLGFLLLSLRQRINPNWPAVFYVPLFILAAAWLEGRLPF
                     PPRSAWKKWSLRVAGSVTALLHLALLVIFLTDLKGHKKLVEMQGWHETGSQAGAFLDD
                     IPNPRRAFVLVAGYRYDAAQLAFHMPQQPRVYRWSKTADVESQYEVWPGPEERLGDDA
                     LIFQPGKEAPAELKAPLRACFENAERLGRIETPLGGGHVRYFDVFLARNLLTWTRAGG
                     MTSAP"
     gene            complement(4054..>4683)
                     /locus_tag="PLP58_20910"
     CDS             complement(4054..>4683)
                     /locus_tag="PLP58_20910"
                     /inference="COORDINATES: protein motif:HMM:NF014684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ11054.1"
                     /translation="AFAALVIAALGFCAWRGRASARAAGFAVPDAETSFLRFFIGAQA
                     VLLFVIITSQFRGVPLPALIFGAVACAVWVLTRHTPFGRHLYAIGGNPEAAFVSGVPV
                     RRVVVGAFALMGGIVAVTGFMLTSYTGNSTPDLGEWMELDAVAACVIGGVSLRGGRGS
                     VIGVIFGALIMAVLLNGMTLMSVTPEHKLVVRGLVLLGAVWLDVRMNRA"
BASE COUNT          848 a         1359 c         1758 g          718 t
ORIGIN      
        1 ggcctgcgcc acgcggccca ggccagcgcg ccggcgagcc agaggaaggg ggccgccttt
       61 aacaaagtga cgctgccgcc gatgccggac atggcgccca gggccagagc gcaggtgagc
      121 gccgtcgccg gccagagtgt ggcgttcacg ctggcgaccc agcggcgcag cacgacccag
      181 gctgtaacgc gccgcgcgac tgaggcgaag gataactggg gggggccggg ttgtgacatg
      241 gggctttcga tgagcataac gagcgccagt cctccgcatt cagcgggaaa tgccgctcct
      301 ggaggatttc agcggacttt gatctctttt tcagtggcgt ccatttcggc ctcccaacgg
      361 gcgaggcggg ctttgaggcc ggtgacgatt tcggggtgct ggtagccgag gttgacgcgc
      421 tctccagggt cgtctttgag atcaaaaagc aggtccatgg tgttgccgtc gttgatgtat
      481 ttccaccggc cgtggcggat ggctttcatc ttgcggttgg cgcggtccat gcgccagcag
      541 agggagcggt cctgagcggc ggcttccgcg ctgagggcgt gggggaggag gtccatgccg
      601 tcgagcggct tgtcctcggg caggggcagg ccggcggcta agagaaaggt ggcgtgcagg
      661 tccatggtga tggcgggggt ttcgcacaca gagcctttgg gcaggcgggc gggccagctc
      721 atgaggcagg gcacgcggat gccgccctcc cagagggtgg ctttgtggtg aaaatagggg
      781 gcgttgctgg cccagcgctc gccgccgttg tcgctggaaa agacgatgag ggtgtcgttg
      841 gtgatgccgt gcgcttcgag ttcggcgagg atcatgccga cgccgtgatc cacgcgctcg
      901 agcatggctt tgtagatggc gcgggatccg gtgagcatgt tttccttggt gaccatgggt
      961 gtccggggcg cgtccggcgg ctggaagggg gcgtggacgg cgaggtgggg gacatagaga
     1021 aagaagggct ttttgcccct gccgccgtgc tggcggatga agcgggcggc gcgctcgttg
     1081 acgaggtcgg tgaagtaacc ctccatttgc acgggctgga gtccgtcccg cagggcgtgg
     1141 gtgccgtcga agtaggtgtg gcggtaaaag tcggcgtggc cgagcagctc gccgaagtat
     1201 tcgtcaaagc cgcggtggag ggggttgtac tcgtccttgt ggccgaggtg ccatttgccg
     1261 aaggcgccgg tggtgtagcc ggcggtcttg aggcgctgtg cgatggtggt ctcgccggcg
     1321 ggcaggccga ggccgtgaac gtcgggctcg ggcacccatt ctccgccgac tttgcgccat
     1381 tgctcgacct ggtagccgaa ggcgaactcg atgccggtgc gctgctgcca gcgcccggtc
     1441 atgaaaccga cgcgggtggg ggtgcagacg ggggcgttgg agtagaagtc ggtgaacttc
     1501 acgccctcgg cggcgaggcg gtctatgtgc ggggtgcgga tgtctttggc gccggtgcag
     1561 ccgaggtcgc cgtagcccat gtcatccacg aggatgaaga tgatgttcgg cggagctgcg
     1621 tgaagggcgg cagcggcaag catggagagg aggaaggcgg cgcggaggtt tttcatctcc
     1681 ggcgagccta ttcaatccgc cgtggctgtg gcaagcgcgg agtgggggct ttttttcgag
     1741 gccaatgagg cggccgtcat ttgatggcgc gcggcgagct ttgcggcggc ccagccggcg
     1801 agacgcacgg ctccccagcc gagcaggatg ccgatgccgg ttgagggaat gacatcgctg
     1861 ggccagtgcg cgcctacata gacgcgggac caggcgacgg cggcggcgaa catgaaggcc
     1921 aggatgcccc agcgccggta aaagagggcg acgacggtgg cgagggcgaa catgttcacc
     1981 gtgtggctgc tggggaagga tttgcggtcg gggctggcct tcctgaagcc gggcttctgg
     2041 acaggcggaa ggaacaggcg gcggaactcg ggctggggag ccttgccgag atcgcgcacg
     2101 acaacttcct ccagggcgtc gcgcggacgg acctggccgg tgtgtttttt gagcacgttg
     2161 cccacgatgc cgtcgccggt gccgacggcg acgccgacgc acaggagcat gagccgggcg
     2221 cgcgcgccgc cgcgccataa aatggcgatg acgaggagaa tgagcagggg cacccaggct
     2281 tgaatgcagg agacggcggg catgagccag tccaggaccg ggtgcgtcca gtcctggttg
     2341 atctttcgca gcagatgcag atcccagtcc atcatggtgc ggaggtcatg ccgcccgccc
     2401 tggtccaagt caaaaggttc ctggcgagaa aaacgtcaaa ataacggaca tgcccgccgc
     2461 cgagaggagt ctcgatgcgt ccgaggcgct cggcattctc gaagcaggcg cggaggggcg
     2521 ctttgagttc ggcgggggct tccttgccgg gctggaaaat gagggcgtcg tccccgaggc
     2581 gctcctcggg gccgggccag acctcgtatt gggactccac atcggcggtc ttgctccagc
     2641 gatagacgcg cggctgctgc ggcatgtgga aggcgagctg ggcggcgtca taccggtagc
     2701 cggccacgag cacgaaagcg cgcctcgggt tcgggatgtc atcgaggaaa gcgcccgcct
     2761 gactgccggt ctcgtgccag ccctgcatct cgacgagttt cttgtggcct ttgagatcgg
     2821 tgaggaagat gacaagcagt gcgaggtgaa gcaaggcggt gacgctgccg gcgacacgca
     2881 ggctccactt tttccacgcg gagcgcgggg ggaatggcag acgcccctcc agccaggcgg
     2941 cggcgaggat gaagagtggg acataaaaga cagcgggcca gttcgggttg atgcgctggc
     3001 gcagggacag cagcaggaag ccaagcagcg gcagcacgga gaagaccagc aggtagagct
     3061 cccggctgcc gagggcgcgc cagtttttca gagacagcca ggccgcaaaa atgagcgtgg
     3121 cgaaggtgac gggcgtgtag accagcgcct gcacggcggg gaactcgagg gtgcgcccga
     3181 gccatttgcc catgccgagg ctctccgtgt cgaagtggtg cctcgtgtgc tccagggtga
     3241 cccactcgtg gcgcgcgttc cacatcagca cgggggtgat gaaggccatg ccggcgagag
     3301 ctgcgagcca gaagccgggg cgggcgagca tggggcggtt gtctttggaa accgcgctga
     3361 aaaccagcgc cagcgcggga aagacgagca tcatctgctt gctcagaacg ccaaagccaa
     3421 gcgcgagcgc cagcacgagc caccaggcgg gctttgcctc ctccgccgcg cgccagagcg
     3481 ccagcatggc gagcgaccag gccagcagca gcggggcgtc tatggtgaaa aaaaggttca
     3541 gcccggcatt gcccggcgtg agcacgatga gcagcgcggc caggcaggcg gcacgcgcgc
     3601 cgtagagatc gcgcgcaagc gagaagacgg cggcgagcgt gcccgtgccc agcagcagcg
     3661 gggcgaggcg gatgccccac tcggcattgc cggtgatcca gcccgtaatg cccatgagcc
     3721 agccgatcat cggcggtttg ctaaaataac cccagtccgg ctggcgcccc cagtcccagt
     3781 aatacgcctc gtctccgagc aggtccgcct gctggcagaa gaagagcatg aaggccaagc
     3841 ggaaggcgaa gactgcggcg agcccccgcc aaaagaggcg ggtccagagg gtgtcgtcgg
     3901 agtctgaaat cactggcaat ggcaaagagc tagcagggta ggctgggttt ccgggctggc
     3961 aagcggggcc ggccttgctt ggcaatggat tgaccgcgca ggattcgagc ctgagtacga
     4021 gctttcttgg gatgcgcaac cttgccggcg ctttcacgcc cggttcatgc gcacatccag
     4081 ccacaccgcg ccgagcagga cgaggccgcg cacgacgagc ttgtgctccg gggtcacgga
     4141 catcagggtc atgccgttga gcagcacggc catgatgagc gcgccgaaaa tgacgccgat
     4201 gacactgccg cgcccgccgc gcaggctgac ccccccgatg acgcaggcgg ccacggcgtc
     4261 gagctccatc cactcgccga ggtcgggggt ggagtttccg gtgtaggagg tgagcatgaa
     4321 gccggtgacg gccacgatgc cgcccatgag cgcgaacgcg ccgacgacga cgcgccgcac
     4381 cggcacgccg gagacaaagg cagcctctgg atttccgccg atggcgtaaa gatgccggcc
     4441 aaagggggtg tggcgcgtca gcacccagac cgcgcaggcc accgcgccga agatgagcgc
     4501 gggcagcggc acgccgcgaa actggctggt gatgatgacg aagagcagca ccgcctgcgc
     4561 gccgatgaaa aagcgcagaa aggatgtctc cgcgtccggc acagcgaatc ccgccgcccg
     4621 cgccgaggcg cgcccgcgcc aggcgcagaa gccgagcgcg gcgatgacca gggcggcaaa
     4681 cgc
//
LOCUS       DAOMYJ010000318         4419 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Prosthecobacter sp. isolate AMR_MDS_5629
            NODE_6963_length_4419_cov_21.184005, whole genome shotgun sequence.
ACCESSION   DAOMYJ010000318 DAOMYJ010000000
VERSION     DAOMYJ010000318.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867850
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prosthecobacter sp. (wastewater metagenome)
  ORGANISM  Prosthecobacter sp.
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Verrucomicrobiaceae; Prosthecobacter.
REFERENCE   1  (bases 1 to 4419)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4419)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.30
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:34:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,742
            CDSs (total)                      :: 4,684
            Genes (coding)                    :: 4,676
            CDSs (with protein)               :: 4,676
            Genes (RNA)                       :: 58
            tRNAs                             :: 55
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4419
                     /organism="Prosthecobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6963_length_4419_cov_21.184005"
                     /isolate="AMR_MDS_5629"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1965333"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(280..1623)
                     /locus_tag="PLP58_20915"
     CDS             complement(280..1623)
                     /locus_tag="PLP58_20915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007418178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HRJ11055.1"
                     /translation="MNTQPAPLTRRRFVGAAGLVAGSMITRPIFGQDAPGNKLNIALI
                     GVWGRGLAHYDAISEENVVALCDVNEERFPEALKRFPGAKTYVDWRKCLDHPGLDAVV
                     ICTTDHTHAFIANWALKRDLHVYCEKPLAITVEEARIVRATWESKKGKLATQVGMQRH
                     AQANFNRVRELIHDGAIGDLKAAYAWGNRQIHKPGYLPEEGAPPPGFHYDLWLGPAPY
                     HPYNPAYFGGRAGANCLSWNMFWDFGAGQIGDMGSHTMDIVWNVVDARLPTSAEAKGE
                     PLNSDVTPVECESHFEHPANDWRGPITVSWYQGGVLPRSPKPWIDFNGIGHGAMFKGS
                     KGFLIADFDTRIILPFGDDADLTYYKPRKKEELLPPVGVFQKQWLDACRDPSKKTACD
                     FGYSADMIEQMLLGLVAYRIGKKIQYDPKTGTSPDTPEANQFMGKEYRKGWSIDG"
     gene            complement(1725..2480)
                     /gene="pyrF"
                     /locus_tag="PLP58_20920"
     CDS             complement(1725..2480)
                     /gene="pyrF"
                     /locus_tag="PLP58_20920"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018968754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004590 - orotidine-5'-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HRJ11056.1"
                     /translation="MTFREKLEKRIENAGTHLCVGLDPRMTKPDEAAKLWLQDVIAQT
                     APYAAAFKPNSAYFEAHGWRGMRLLEMILKFIPGDIPVVLDVKRGDIGETQAFYAKAA
                     FDQMRVDAVTLNPYMGSDTLAPFLEYPDKGIYLLGVTSNPGAADIELQNCGGRHVYEL
                     VAVMTRASRQAGLVLGLTNAAPDVLQRIPDVPLLIPGLGAQGGDLSALKDSGRLAPIL
                     VNVSRGILYHGQEGRTYGEVAREWSEKIEHALA"
     gene            2564..3079
                     /gene="purE"
                     /locus_tag="PLP58_20925"
     CDS             2564..3079
                     /gene="purE"
                     /locus_tag="PLP58_20925"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018290900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009320 - phosphoribosylaminoimidazole
                     carboxylase complex [Evidence IEA];
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HRJ11057.1"
                     /translation="MDQPLVGIIMGSSSDWPTLENAARVLEDFGVPYEKKVVSAHRTP
                     QLLYEYASSAAGRGLKCVIAGAGGAAHLPGMTASMTTLPVLGVPVQSRALNGLDSLYS
                     IVQMPGGVPVATFAIGNAGALNAALFAVSMLANNDPALAAKLQAFRERQTAKVLESQK
                     ELDAPAGGDVR"
     gene            3128..4267
                     /locus_tag="PLP58_20930"
     CDS             3128..4267
                     /locus_tag="PLP58_20930"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: protein motif:HMM:NF004677.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HRJ11058.1"
                     /translation="MFLPPSTLGILGGGQLGRMLALEARRSGYRVVIFTDEPPGCPAG
                     QFADLEINAAYDDRAALDRFLSAVDVVTAEFENIPDSCLQAVEAVKPLRPSRAAIHTT
                     QHREREKLFLRRQGIACADFRIVNDLPGLERAVAELGRPCVIKTAAFGYDGKGQRKVV
                     EDTELAQAWRDFEGHNAVVEQWVPFACEVSVVGARGADGGMAVHGVVENWHLHHILDV
                     TIAPARVTPAVMKQALQLWRAVAEGLDYAGTMAVEMFVTRSEKVLVNEIAPRPHNSGH
                     YTIDACVTNQFQQQLRAVCGLPLGDPSQHTPAVMVNLLGDVWPSETRHPDWSPVLAHP
                     RAKLHLYGKNAARPRRKMGHFTVLGNTRDEALLAARQLRFALGIE"
BASE COUNT          757 a         1390 c         1456 g          816 t
ORIGIN      
        1 gtagcctgtc tcctcccagc cgtggtcgtc gcccatcatg aggatgatgt tcggcggggc
       61 gctcagggcg gtgatgaaag gcgtgagtgc gaggaaaatg aggacggcgt ggttcattcg
      121 gcttcgggtt gactgtgagg ctggctctgc caggcagggg tcaacgacct gcgcgcgcac
      181 gactttcctc aaatgaacgg tgccaggcac catgaccagc aacaaaaaca tccgtcaccc
      241 aacggtgacg gatgttggca gccggcgtct cttttggctt cagccgtcaa tgctccagcc
      301 tttgcgatac tccttgccca tgaactggtt ggcctccggc gtgtcggggc tggtgccggt
      361 cttggggtcg tattggattt tcttgccgat gcggtaggcc acgaggccga ggagcatctg
      421 ctcgatcatg tccgcgctgt agccgaagtc gcaggcggtc ttcttcgaag ggtcgcggca
      481 ggcgtcgagc cattgttttt ggaagacgcc gacgggcggc agcaactcct ctttcttgcg
      541 cggcttgtag taggtgaggt cggcatcgtc gccaaagggc aggatgatac gggtgtcgaa
      601 gtcggcgatg aggaacccct tgctgccttt gaacatggcg ccgtggccga tgccgttgaa
      661 atcaatccag ggctttggcg agcgcgggag cacgccgccc tgataccagc tcacggtgat
      721 gggcccgcgc cagtcgttgg cgggatgctc gaaatgggac tcgcactcca cgggtgtcac
      781 atcggagttg agaggctcgc ccttggcctc ggcggaggtg ggcaggcgcg cgtccacgac
      841 attccagaca atgtccatgg tgtggctgcc catgtctccg atctggccgg cgccgaagtc
      901 ccagaacatg ttccaggaaa ggcagttggc tccggcgcgc cctccgaagt aggcggggtt
      961 gtacggatga taaggggcgg ggccgagcca gagatcgtag tggaagccgg gcggcggagc
     1021 gccctcctcg ggcaggtagc cgggcttgtg gatctggcgg ttgccccagg cataggcggc
     1081 tttgaggtcg ccaatggcac cgtcgtgaat gagctcgcgc acgcggttga agttggcctg
     1141 ggcatggcgc tgcatgccga cctgggtggc cagcttgccc tttttgctct cccaggtggc
     1201 gcggacgatg cgggcctcct cgacggtgat ggccagcggc ttttcacaat agacgtgcag
     1261 gtcgcgtttg agcgcccagt tggcgatgaa ggcgtgcgtg tggtctgtgg tgcagatgac
     1321 cacggcgtcg agtccgggat gatccaggca cttgcgccag tccacataag tcttggcgcc
     1381 ggggaagcgc ttcaatgcct ctgggaatcg ctcctcgttc acatcgcaaa gggcgacgac
     1441 attttcctcg ctgatggcgt cgtaatgcgc gaggccacgg ccccagacac cgataagggc
     1501 gatgttgagt ttgttgcctg gcgcgtcctg gccgaagatg ggccgggtga tcatggagcc
     1561 ggcgacgagg ccggccgcgc cgacgaaacg gcggcgggtc aagggtgcgg gttgggtgtt
     1621 catggcagga aggaaaacga cacccgccgc ggcgatagcg caagcaatgt tcctgcgcgt
     1681 cacctcgcct tgcgcatttt cgcgctccca caccgccaaa gcgatcaagc aagcgcgtgc
     1741 tcgatcttct ccgaccactc gcgcgccacc tcgccataag tgcggccttc ctggccgtgg
     1801 tagaggatgc cgcgcgagac gttcaccaga atgggcgcga ggcgtccgct gtccttgagc
     1861 gcggagaggt ctccgccctg cgcgccgagg ccggggatga gcagcggcac atccgggatg
     1921 cgctggagca catccggcgc ggcattggtg aggccaagca cgagtcctgc ctgacggctg
     1981 gcccgcgtca tgacggccac gagttcataa acatggcggc cgccgcagtt ctgcagctcg
     2041 atgtccgccg cgccgggatt ggaggtgacg ccgaggaggt agatgccttt gtcaggatac
     2101 tccagaaagg gggcaagcgt gtcgctgccc atgtagggat tcagcgtcac ggcgtccacg
     2161 cgcatctgat cgaaggcggc tttggcgtaa aaggcctgcg tttcgccgat gtcgccgcgc
     2221 ttcacatcga gcaccacggg gatgtcgccg gggatgaact tcaaaatcat ctccagcagc
     2281 ctcatgccgc gccagccgtg ggcctcaaag taggcggagt tcggcttgaa ggcggcggcg
     2341 tagggggcgg tctgcgcgat cacatcctgc agccagagtt tcgccgcctc gtcaggcttg
     2401 gtcatgcgcg gatcgagtcc cacgcacaga tgcgtgccgg cattctcgat tcgtttctcc
     2461 aatttttcac ggaaagtcat gcgggcggac attgaatcgc cacggcggca tggcaaccgc
     2521 aagcatggct tgcagggctg aaatgtccgg cttatctcgg cgcatggatc aaccccttgt
     2581 cggcatcatc atgggctcca gctccgactg gcccaccctc gaaaacgcgg cgcgcgtgct
     2641 tgaggacttc ggcgtgccct atgaaaaaaa agtggtcagc gcgcaccgca cgccgcagtt
     2701 gctttatgaa tacgcctcca gcgccgccgg gcgcgggctg aaatgcgtca tcgccggcgc
     2761 gggcggcgcc gcgcatctgc cgggcatgac ggccagcatg accacgctgc cggtgctggg
     2821 agtgccggtg cagagccgcg cgctcaacgg actcgacagc ctctactcca tcgtgcaaat
     2881 gcccggcggc gtgccggtgg ccacctttgc catcggcaac gcgggcgctc tcaacgccgc
     2941 gcttttcgcc gtctccatgc tcgccaacaa cgaccccgcc ctggccgcca aactccaggc
     3001 cttccgagag cgccagacgg ccaaggtgct ggagagccag aaggaactcg acgcgcccgc
     3061 cggaggtgat gtccgatagc cgatagccaa cggccctttt ctcatttcca tttgcccacc
     3121 gcccaccgtg ttcctccccc cctccaccct aggcatcctc ggcggcggcc agctcgggcg
     3181 aatgcttgcc ctggaagcgc gccgcagcgg ttatcgtgtc gtcattttca cggacgagcc
     3241 gccaggctgc ccggcggggc agttcgccga tcttgaaatc aacgcggcct atgacgaccg
     3301 cgcggctttg gaccgcttcc tctccgctgt ggatgtggtc acggcggagt ttgaaaacat
     3361 cccggactca tgcctccagg ccgtcgaggc ggtgaagccg ctgcgccctt cgcgcgccgc
     3421 catccacacc acccagcatc gcgagcgtga aaagctcttc ctgcggcggc agggcatcgc
     3481 ctgcgcggac tttcgcatcg tcaacgacct ccccggcctg gagcgcgccg tggctgagct
     3541 gggccgtccc tgcgtcatca aaacggccgc ctttggctac gacggcaaag gccagcgcaa
     3601 ggtcgtggag gacaccgagc tggcccaggc gtggcgggac tttgaagggc ataacgccgt
     3661 ggtcgagcaa tgggtgcctt tcgcgtgcga ggtgtccgtc gtcggcgcgc gcggagcgga
     3721 tggcggcatg gccgtgcatg gcgtggtgga gaactggcac ttgcaccaca ttctggatgt
     3781 caccatcgcc ccggcgcgtg tgactcccgc cgtcatgaaa caagccctgc aactctggcg
     3841 cgccgtggcg gaggggctgg actacgcggg cacgatggct gtggagatgt tcgtgacccg
     3901 ctcggaaaaa gtgctcgtca acgagatcgc cccgcgtccg cacaacagcg ggcattacac
     3961 cattgacgcg tgcgtcacga accagttcca gcagcagttg cgcgcggtgt gcggcctgcc
     4021 gctgggcgac ccctcccagc acacgcccgc tgtcatggtg aacctgctcg gcgatgtgtg
     4081 gccctcggaa acacggcatc ccgactggtc cccagtgctc gcgcatccgc gcgccaaact
     4141 gcatctttac ggcaagaacg cagcccggcc acgccgaaag atggggcact tcaccgtgct
     4201 gggaaatacg cgggatgaag ccctgctcgc agcccgccag ttgcgtttcg cgctcggcat
     4261 cgagtgaaat ggagacacta tcagcgctcc aagcctcaca cgaccacgtt caccagcttc
     4321 cccggcacga cgatgatctt cttggcgggc ttgccctcca tgaattcctg cacgcgggcg
     4381 ctggcgaggg cgattttttc gatctcctcc tttgtggcc
//
LOCUS       DAOMYJ010000319         4313 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Prosthecobacter sp. isolate AMR_MDS_5629
            NODE_7175_length_4313_cov_19.226632, whole genome shotgun sequence.
ACCESSION   DAOMYJ010000319 DAOMYJ010000000
VERSION     DAOMYJ010000319.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867850
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prosthecobacter sp. (wastewater metagenome)
  ORGANISM  Prosthecobacter sp.
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Verrucomicrobiaceae; Prosthecobacter.
REFERENCE   1  (bases 1 to 4313)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4313)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.30
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:34:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,742
            CDSs (total)                      :: 4,684
            Genes (coding)                    :: 4,676
            CDSs (with protein)               :: 4,676
            Genes (RNA)                       :: 58
            tRNAs                             :: 55
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4313
                     /organism="Prosthecobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7175_length_4313_cov_19.226632"
                     /isolate="AMR_MDS_5629"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1965333"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..900)
                     /locus_tag="PLP58_20935"
     CDS             complement(<1..900)
                     /locus_tag="PLP58_20935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ11059.1"
                     /translation="MKIAPSLKLALGTALLAAPAAQAGTNVWFTPLTQSAPIVAPNSI
                     EELDGPWTTPEGILQNNRVSMREVEDAILSPGQSIVRVPGAGTSACMIDMLAFNKTAT
                     HLFLPHETPTGAGVSRYDIYANKCEVIFAGDATGNWASDYGAFDPCRWTPNGTIFLGE
                     EWSGEGRLIEILNPMADVSDIQFRELNSIANVSHEGINFSNRYRNTIYFIDENNSGSI
                     YKFVMKKTGDYTAGQTFVLSVNAFAATGGVASQNYNSTANTAATRTGAATWVPLTDRD
                     GNPLPGIPNPFQNGPTGSASLGGR"
     gene            1129..3012
                     /locus_tag="PLP58_20940"
     CDS             1129..3012
                     /locus_tag="PLP58_20940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018969518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HRJ11060.1"
                     /translation="MLSASELTLSFGNQRLLEGVTFAVSDGEKAGLVGRNGCGKTSLL
                     KILSGQERPDSGELSMRRGLRTGYLPQEFELDDAATVLENIQSGAADLIEWLGRYEAG
                     AGSGAELEQIQHQIEAADGWNLRARIKTIATALAAPPLDAPVAPLSGGEKRRVALCRA
                     LVSQPELLLLDEPTNHLDSESIRWLEDYLRGFPGAVIFVTHDRYFLDVIATRILELAD
                     GRCYSHEGNYTAYLESKAARRQIAEQTERRRQRFLRTELEWVRAGVKARTTKSRHRLE
                     MFYQIEGMEAPPEEREMDLLMPPPPALGDIAVNLEDAGARVTDEAGRERWLFRHLEVM
                     LRPGQCTGIVGRNGAGKTTLLRLCMGERAPDEGRVTVGKKVVINYIDQARMTLDGAGS
                     VLAEVADQDETVFFGAQKLGVRAYLRRFLFNDDRINERVDRLSGGERARLMLAKVLKR
                     GGNLIVLDEPTNDLDLQSLRILEEALSDFDGTVLVVSHDRYFLDRVCDQIIAFEDGGV
                     HVQVGNYSYYLEKRRERETRERMWTSAAKPAKAAPAPKARPRKLTFKEARELETIEAA
                     ILAAEERAGALDATLNDPAFYITRSHEAEGLIKDLEAAKTEVTRLYARWEELEAVKAE
                     GGP"
     gene            3034..3819
                     /gene="hisF"
                     /locus_tag="PLP58_20945"
     CDS             3034..3819
                     /gene="hisF"
                     /locus_tag="PLP58_20945"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009958765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisF"
                     /protein_id="HRJ11061.1"
                     /translation="MPRHPGLPPMLTKRIIPCLDVKEGRVVKGTQFLQLRDAGDPVEC
                     AKVYNAQGADELVFLDITASHEERKTMADVVARTAESCFMPLTVGGGIRTVADMREML
                     LAGADKVGINTAAVKTPDVIDAAAEAFGCQCLVVAIDAKRNDRGSWTVFTHGGRNATA
                     LDAVEWAAEVCRRGAGEILLTSMDADGTKAGFDIALTRAVSEAVTIPVIASGGAGSLQ
                     HMADVLSQGKADAVLAASIFHFGEHTVGDVKHFLAGHGVPVRL"
BASE COUNT          722 a         1506 c         1397 g          688 t
ORIGIN      
        1 gcggccgccg agggaggcgc tgccggtggg gccgttttgg aaggggttcg ggatgccggg
       61 cagcgggttg ccgtcgcggt ccgtcagcgg cacccaggtg gccgcgccgg tgcgggtggc
      121 ggcggtgttg gctgtgctgt tgtagttctg cgaggccaca ccaccggtgg cggcgaaggc
      181 gttcacactg agcacgaagg tctggccggc ggtgtagtcg ccggttttct tcatgacgaa
      241 cttgtagatg gagccggagt tgttctcgtc aatgaagtag atggtgttgc ggtagcggtt
      301 gctgaagttg atgccctcgt gcgagacgtt ggcgatgctg ttcagctcgc ggaactggat
      361 gtcgctcaca tccgccatcg gattgaggat ttcaatgagg cggccttcgc cggaccattc
      421 ctcgccgagg aaaatggtgc cgttgggcgt ccagcggcac gggtcgaagg cgccgtagtc
      481 gctggcccag ttgccggtgg cgtcgcccgc gaagatgacc tcgcacttgt tggcgtagat
      541 gtcgtagcgc gagacgcccg cgccggtcgg ggtctcgtgc gggaggaaga ggtgggtggc
      601 ggtcttgttg aaggccagca tgtcaatcat gcaggcgctg gtgccggcgc cggggacgcg
      661 gacgatggac tggccggggc tgaggatggc gtcctccacc tcgcgcatgg agacgcggtt
      721 gttttggagg atgccttccg gcgtggtcca ggggccgtcg agttcctcga tggagttcgg
      781 agccacgatg ggggcggact gggtcagcgg ggtgaaccag acattggtgc cggcctgggc
      841 ggcgggcgcg gccagcaggg ccgtgccgag ggcgagtttg agcgagggtg cgattttcat
      901 gttgttgggt ggtgtgttgc gccggctgtt gatgcgccgg cgcgccgctg ttacccgcct
      961 cttgcgggac cgtcgcgccg aataagttga cgttatcgcc atgtggcaaa accggcacga
     1021 ggctggcgga taatgtcgcg caatgtaact tgacgcgccc gccgccggcg gcggcgcgcc
     1081 atcatgcgcg cttggcaaag ccccccgcat ggtccaggga atgcgcccat gctttccgcc
     1141 agcgaactca ccctctcctt cggcaaccag cgcctgctcg aaggcgtcac cttcgcagtc
     1201 tccgacggcg agaaggcggg cctcgtcggc cgcaacggct gcggcaagac cagcctgctg
     1261 aaaatcctct ccggccagga gcgccccgac agcggggagc tgtccatgcg gcgcgggctg
     1321 cgcaccggct atctgcccca ggagttcgag ctggatgatg ccgccaccgt gctggaaaac
     1381 atccagtccg gcgcggcgga cctcatcgaa tggctcggcc gctacgaggc cggcgcgggc
     1441 agcggggcgg agctggagca gatccagcac cagatcgagg ccgccgacgg ctggaatctc
     1501 cgcgcacgca tcaaaaccat cgccaccgcc ctggccgccc cgcctctgga cgcgcccgtg
     1561 gccccgctct ccggcggcga aaaacgccgc gtcgccctct gccgcgccct cgtctcgcag
     1621 ccggagctgc tcctgctcga cgagccgacc aaccacctcg actccgagtc catccgctgg
     1681 ctggaggatt acctgcgcgg ttttcccggc gcggtcatct tcgtcaccca tgaccgctat
     1741 ttcctcgatg tcatcgccac ccgcatcctg gagctggcgg acgggcgctg ctactcccac
     1801 gaggggaact acaccgctta tctcgagagc aaggcggcgc gccgccagat cgccgagcag
     1861 accgagcggc ggcggcagcg cttcctgcgc accgagctgg agtgggtgcg cgccggggtg
     1921 aaggcgcgca ccaccaagtc acgccaccgg ctggaaatgt tttaccagat cgaaggcatg
     1981 gaggccccgc ccgaggagcg ggagatggat ctcctcatgc cgccgccgcc cgccctcggg
     2041 gacattgccg tgaacctgga ggacgccggc gccagagtca ccgacgaggc gggccgggag
     2101 cgctggcttt tccgccatct ggaggtcatg ctccgccccg gccagtgtac aggcatcgtc
     2161 ggccgcaacg gcgcgggaaa gaccaccctg ctgcgcctct gcatgggcga gcgcgcgccg
     2221 gacgagggcc gcgtaaccgt ggggaaaaaa gtcgtcatca actacataga ccaggcgcgc
     2281 atgacgctcg acggcgccgg cagcgtgctc gccgaggtgg ccgaccagga cgagaccgtc
     2341 ttcttcggcg cgcaaaagct cggcgtccgc gcctacctgc gccgcttcct tttcaatgac
     2401 gaccgcatca acgagcgcgt ggaccgcctc tccggcggcg agcgcgcccg cctcatgctg
     2461 gccaaagtcc tcaagcgcgg cggcaacctc atcgtcctcg acgagcccac caacgacctg
     2521 gatctgcaaa gcctccgcat cctcgaggag gccctcagcg acttcgacgg caccgtgctc
     2581 gtcgtcagcc acgaccgcta cttcctcgac cgcgtctgcg accagatcat cgccttcgaa
     2641 gacggcggcg tgcatgtgca ggtgggcaac tacagctact atctggaaaa acgccgcgag
     2701 cgcgaaaccc gcgagcgcat gtggaccagc gccgccaagc ccgccaaagc cgcccccgcg
     2761 cccaaagcca gaccgcgcaa gctcaccttc aaggaggcgc gcgagctgga aaccatcgag
     2821 gccgccatcc tcgccgccga ggagcgcgcc ggcgccctcg acgccaccct caacgacccc
     2881 gccttctaca tcacccgctc ccatgaagcc gagggcctca tcaaggacct ggaggccgcc
     2941 aaaaccgaag tcacccgcct ctacgcccgc tgggaggagc tggaggccgt gaaagcggaa
     3001 ggcggccctt gaagcgggcg ttgatttgaa aaaatgccgc gccatcctgg cctccccccc
     3061 atgctcacca agcgcatcat tccctgcctc gatgtcaaag aaggccgtgt cgtcaaaggc
     3121 acgcaattcc tccagctccg cgacgccggc gaccccgtgg aatgcgcgaa agtttacaac
     3181 gcccagggcg ccgacgagct ggtctttctc gacatcaccg ccagccacga ggagcgcaag
     3241 accatggccg acgtcgtcgc ccgcaccgcc gagagctgct tcatgcccct caccgtcggc
     3301 ggcggcatcc gcaccgtcgc cgacatgcgc gaaatgctgc tggccggcgc ggacaaggta
     3361 ggcatcaaca ccgccgcagt gaaaacgccc gatgtcatag acgccgccgc cgaggccttc
     3421 ggctgccagt gcctcgtggt ggccattgac gccaagcgca acgaccgcgg ctcctggacc
     3481 gtcttcaccc acggcgggcg caacgccaca gcgctcgacg ccgtcgaatg ggccgccgaa
     3541 gtctgccggc gcggcgcggg cgagatcctc ctcaccagca tggacgccga cggcaccaag
     3601 gccggctttg acatcgccct cacccgcgcc gtcagcgagg cggtcaccat ccccgtcatc
     3661 gccagcggcg gcgccggcag tctccagcac atggccgatg tgctcagcca gggcaaagcc
     3721 gacgccgtgc tcgccgccag catcttccac ttcggcgagc acaccgtcgg ggacgtgaag
     3781 cacttcctcg ccggccacgg cgtccccgtg cggctttgat tgccccccgg cacgggtttt
     3841 gcctccggcc aacccgcggg gggagttcat ttcgtgcgcg cctactgtca tcgtgcgtcc
     3901 gctctccacg gcttggggcg gattgagcct gcggacgttc cactggcttc cccccacagg
     3961 tcccggcgcg cccggttcag gcatccggct cctcgtgcca cggtttcaca tgaatgaccg
     4021 tccgcgccgg ccatgtctgg cgaaaaaaaa atccagcccc ctgtaaccgc cgccgccgga
     4081 acccttagat gatggtgtgg ggatgctctc tcccctgacc gccatgatcg caccctccat
     4141 cctcatgccg ctcctgccac tcccgccagc cgcgcgccct ggcgttcttc caaaacggca
     4201 gccagttgac gcaaaaccgc cgcccgtttt cgcaaaaccg ccgacggttt cctcagaact
     4261 gccgcctgtt tcctcagaac tgccgacggt ttccgcaaaa ccacagacgg ttt
//
LOCUS       DAOMYJ010000320         4309 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Prosthecobacter sp. isolate AMR_MDS_5629
            NODE_7185_length_4309_cov_21.129525, whole genome shotgun sequence.
ACCESSION   DAOMYJ010000320 DAOMYJ010000000
VERSION     DAOMYJ010000320.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867850
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prosthecobacter sp. (wastewater metagenome)
  ORGANISM  Prosthecobacter sp.
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Verrucomicrobiaceae; Prosthecobacter.
REFERENCE   1  (bases 1 to 4309)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4309)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.30
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:34:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,742
            CDSs (total)                      :: 4,684
            Genes (coding)                    :: 4,676
            CDSs (with protein)               :: 4,676
            Genes (RNA)                       :: 58
            tRNAs                             :: 55
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4309
                     /organism="Prosthecobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7185_length_4309_cov_21.129525"
                     /isolate="AMR_MDS_5629"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1965333"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            324..1586
                     /locus_tag="PLP58_20950"
     CDS             324..1586
                     /locus_tag="PLP58_20950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ11062.1"
                     /translation="MANPRAFFQTHFHGLLAALAVAAVYSTLAVLRHSDALIWDEGRY
                     LDCARNMTRGFYATDDNPDFVNGPGYPIVLLPFVLAGAEGLLPARLLNAFFMAGAAWF
                     AWLLLRHYAGAAWAAAVAWLTGFHPTLLWMGFAIMTEPLAMFCMAGFAWSFCAAVRGG
                     GRGMMLTAAFLLGWLTLTRVFFGHVIMAAALPCLALLPFWKSWRHVLARTLVILGGAF
                     LLCTPWLVHTWQKTGRVLCWSTNSGELLYWMTSHHEGENGHWFTTEDAVAHPQLAPNH
                     KDFYERVMRMPILQRDAFLMEAAMANLRADPARVAYNWACNLSRMAFGFPRSFLPEEL
                     RTVVLIAVNGPVIALASLLGLVGLWRWRSVPPEVWLLAVFAAIYLGGGSVAPALPRYF
                     ALMLPVLMLGIATVWSRNVRLSLMKSAG"
     gene            complement(1660..2523)
                     /locus_tag="PLP58_20955"
     CDS             complement(1660..2523)
                     /locus_tag="PLP58_20955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009961829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HRJ11063.1"
                     /translation="MAKIQFGSEVYTWFMQGTGKGYDNKLGHMIKVAAEAGFTGIEPM
                     VLEISESALGCCKYWLGDFCDPARLKDTLQQHNMQLAGLALVCAWDGDTEAPNEREAA
                     DFTIGLLKHFPGALLGTVTLPSGRAKDLQKRRLNVARNVNAVSQRAADQGIKCSYHPN
                     SPPSSLVRTQEDYDVVLSSLDPKVTGWTPDVGHIIRGGMDVIATLSKWQHLINHIHYK
                     DFSGNGAEPWAQMGTGKLDFHKITEFLVQRNYEGWIICEDEAHIAVEDPDGVTRQNGE
                     WCKANLHPLVA"
     gene            2707..3564
                     /locus_tag="PLP58_20960"
     CDS             2707..3564
                     /locus_tag="PLP58_20960"
                     /inference="COORDINATES: protein motif:HMM:NF013760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HRJ11064.1"
                     /translation="MHSIHLAVGPGLHGLQFGFEHLTPPGMAICGVLVFLSVISWTAV
                     LAKWMFLRRSEKANRRFLEKYRASPQPLALYLTRERVEQAPMYHIYHEACRELAFYLV
                     GEEEPGRAFSSRLQGAGRINASQMSAVHVAMERAVAAAATRLEARIGFVATALSAAPL
                     LGLLGTVWGILEVFAALASGEAAPTVAALAPGIASALITTLLALLVALPSLVCYNGLA
                     GRIRRMVVRLDNFAADLNGMLDRHFVDHHAPDESLPSLGGMGAPSLSVHSQPPGGALA
                     SMVKLSAAS"
     gene            3561..3971
                     /locus_tag="PLP58_20965"
     CDS             3561..3971
                     /locus_tag="PLP58_20965"
                     /inference="COORDINATES: protein motif:HMM:NF014525.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HRJ11065.1"
                     /translation="MKRFSNRHLPRMIAGVSPMPLLDAAAVLLLVFLVLMVLPEKGVS
                     ITAAAAQPAETPSHVARLAINRDLEFSLDGAPVAAAELEQALRARVEKQPGLGVVVNL
                     PGNFASRSLSMIMETLRRVGVSRTALEYPDAAPP"
BASE COUNT          640 a         1322 c         1463 g          884 t
ORIGIN      
        1 gggttgtgga ttccaaggtc agcccccatg tcgcggccgc gagcacggcg gcgagaaggc
       61 atccagtcca tctgtgtttg gtggtcagca tagatatttc ggtttgagtt ggtgtgatgg
      121 cggctacggg aaccgttaca aaaaatcatg gttagcaggg ggcgtgccaa agttggattt
      181 cgatctttcc cgccgggccg cggaggggtt gcctaactaa aaccgccgtc gtgatcgagg
      241 ccgctttcga ttgatgacgt acacagccgg cagccgctga aaaacccgct ggcgcgcggg
      301 tgggatgtcc ctttgagtac ttgatggcaa atccccgcgc tttttttcaa acccactttc
      361 acggactcct ggccgcgctt gccgtggccg ctgtttattc cacgcttgca gtgctgagac
      421 actcggacgc gctgatttgg gacgagggcc gttatctgga ttgcgcgcgc aacatgacgc
      481 ggggttttta cgccacggat gacaacccgg attttgtcaa cggaccgggg tatcccatcg
      541 tgctgctgcc ttttgttctg gcgggggccg agggtttgct gcccgccagg ctgctgaacg
      601 cttttttcat ggctggcgcg gcatggttcg cctggctgct tctgcgccac tacgcgggcg
      661 cggcctgggc tgccgccgtg gcgtggctga cgggtttcca tcccacgctg ttgtggatgg
      721 gtttcgccat catgaccgag cctctggcga tgttctgcat ggcgggtttc gcgtggagct
      781 tctgcgcggc cgtgcgcggc ggcggcaggg gcatgatgct gacggcggca tttctgcttg
      841 gctggctgac tctgacgcgc gtgttcttcg gccatgtcat catggcggcc gcgctgccat
      901 gcctggcgct gctgccgttt tggaaaagct ggcggcatgt gctggcgcgg acgctggtca
      961 tccttggcgg cgcttttttg ctctgcacgc cctggctggt ccacacatgg caaaaaacgg
     1021 gccgcgtctt gtgctggtcc acgaacagcg gcgagctact ctattggatg acgagtcatc
     1081 atgaggggga aaacggccac tggttcacca ccgaggacgc cgtggcgcat ccgcaactcg
     1141 cgccgaacca caaggatttt tacgagcgcg tgatgaggat gcccatcctt cagcgcgacg
     1201 cctttctcat ggaggcggcg atggcgaacc tgcgcgctga ccccgcccgc gtggcctaca
     1261 actgggcgtg caatctcagc cgcatggctt tcggctttcc tcgatccttc ctgcccgagg
     1321 agctgcgcac ggtggtgctc atcgcggtga acggcccggt gatcgcgctg gcctcgcttc
     1381 ttgggctggt cggtctctgg cgctggcgct cggtgccgcc ggaagtctgg ctgctggcgg
     1441 ttttcgccgc catctatctc ggcggcggca gcgtggcccc ggctctgccg cgctattttg
     1501 cgctcatgct tccagtcctg atgctgggca tcgccacggt gtggagcagg aacgtgcggc
     1561 tctctttgat gaagtccgcc gggtgagcgc cgccggccaa acaaaaagcg gcagccccgc
     1621 gagaaggctg ccgctgggtg aagggaaagt ccgcccgtat caggcgacca gagggtgcag
     1681 gttggccttg caccactcgc cgttctgccg ggtcacgcca tcggggtctt cgacggcgat
     1741 gtgcgcctcg tcctcgcaga tgatccagcc ttcataattc ctctgcacga ggaactcggt
     1801 gatcttgtgg aagtcgagct tgccggtgcc catctgcgcc cagggttcgg cgccgttgcc
     1861 ggagaagtcc ttgtaatgga tgtggttgat gaggtgctgc cacttggaga gtgtggcgat
     1921 gacatccatg ccgccgcgga tgatgtggcc gacatcaggc gtccagccgg tgaccttcgg
     1981 gtcaaggctg ctcaaaacca cgtcgtaatc ctcctgcgtg cgcacgaggg aggatggcgg
     2041 ggagttgggg tggtaggagc atttgatgcc ctggtcggcg gcgcgctggg acacggcgtt
     2101 gacgttgcgg gccacattaa gccggcgctt ttgcaggtct ttggcgcgcc ctgagggcag
     2161 ggtgacggtg ccgagcaagg cgccggggaa gtgcttcagc aggccgatgg tgaagtcggc
     2221 ggcctcgcgc tcgttcggag cctcggtgtc gccgtcccaa gcgcagacga gcgccaggcc
     2281 ggcaagttgc atgttgtgct gctggagggt gtccttgagc ctggcgggat cgcagaaatc
     2341 acccagccag tatttgcagc agccgagcgc gctctcggag atttcgagca ccatgggctc
     2401 gatgccggtg aagcccgcct cggcggcgac cttgatcatg tggccgagtt tattgtcgta
     2461 gcccttgccc gtgccctgca tgaaccaggt gtaaacttcg gagccgaatt ggatttttgc
     2521 catgtggatg aagtggtttg gttgatctcg atgcgacgcc ccgtggcggc gcgtgcgcat
     2581 tggagacgaa ggcggaaaaa atgtccagcg ttgaattgcc gggggctgtc cgcgcgcctt
     2641 gattcagatt cgggcattga cgcggcgcgg gcttgccgct ttatagcggg cgaatttcac
     2701 acgctgatgc actccattca tttagccgtc ggaccgggcc ttcacggtct tcaatttggc
     2761 tttgagcatc tgacgccgcc tggcatggcg atctgcgggg tgctcgtttt tctttccgtc
     2821 atcagttgga cggcggtgct ggccaaatgg atgtttctcc ggcgctcgga gaaggccaac
     2881 cggaggttcc tggaaaaata ccgcgccagt cctcagccgc tggcgcttta cctgacgcgc
     2941 gagcgtgtgg agcaggcgcc gatgtatcac atctaccacg aggcatgccg ggagctggct
     3001 ttttatctgg tgggcgagga ggagccgggg cgcgcgttca gcagccggct ccagggggcg
     3061 ggccgcatca atgcctcgca gatgagcgcg gtgcatgtcg ccatggagcg cgcggtggcc
     3121 gcggcggcaa cgcggcttga ggcgcgcatc ggttttgtgg cgaccgccct ctcagccgcc
     3181 ccgctgctgg gtctgctggg cacggtgtgg ggcatcctgg aggttttcgc cgcgcttgcc
     3241 tcgggggagg cggcgcccac ggtcgcggca ctggcgccag ggatcgcctc ggcattgatc
     3301 accacgctgc tggcgctgct ggtggcgctt ccgagcctgg tctgctacaa cggcctcgcg
     3361 gggcgcatcc gccgcatggt ggtgcggctg gacaacttcg cggcggacct gaacggaatg
     3421 ctggaccggc attttgtgga ccaccacgcg ccggatgaaa gcctgccgag ccttggcggc
     3481 atgggcgcgc cctccctgtc cgttcacagc cagccgcccg gcggcgctct ggcctcgatg
     3541 gtgaagctct ccgccgcgtc atgaagcgtt tttccaaccg ccacctgccg cgcatgatcg
     3601 cgggcgtgag tcccatgccc cttcttgacg ctgcggcggt gctgctgctg gtcttcctgg
     3661 tgctgatggt gttgcctgaa aaaggggtgt ccatcactgc cgccgcggcg cagccggcgg
     3721 agactccgtc tcatgtggcg cggctggcca tcaaccgcga cttggagttc agcctggatg
     3781 gcgctcccgt ggccgccgcc gaactggagc aggcgctgcg cgcgcgggtg gaaaagcagc
     3841 ccggcctcgg agtggtggtg aacctgcccg gcaacttcgc aagccgctcg ctttccatga
     3901 tcatggagac gctgcgccgc gtcggtgtga gccgcaccgc gctggaatac ccggacgcgg
     3961 cccccccctg agcgcccatg ccagccagcc gccgaactct ggaaccgacg ggagccgtgt
     4021 gcgcgctggc tctcctgggg cacatgctgg caggcgccgg actgtatttc atgctgcgga
     4081 aaaaccgccc tgagccggtg gaaagcgttc atcaaccctg gttccagccc gccgattttc
     4141 tgacgccctc gtgggctgcc gccacaccgc aggcagactc accgaaagcg cccgccgctc
     4201 cggcaacctc gtcacccgcg cctgccgcgc cggagactcc caccccgccc gcacgatggc
     4261 gaccaggtcc aagtcttcgg cggagtctcc gtctatgagc cgcgcgggc
//
LOCUS       DAOMYJ010000321         4308 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Prosthecobacter sp. isolate AMR_MDS_5629
            NODE_7187_length_4308_cov_22.675053, whole genome shotgun sequence.
ACCESSION   DAOMYJ010000321 DAOMYJ010000000
VERSION     DAOMYJ010000321.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867850
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prosthecobacter sp. (wastewater metagenome)
  ORGANISM  Prosthecobacter sp.
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Verrucomicrobiaceae; Prosthecobacter.
REFERENCE   1  (bases 1 to 4308)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4308)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.30
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:34:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,742
            CDSs (total)                      :: 4,684
            Genes (coding)                    :: 4,676
            CDSs (with protein)               :: 4,676
            Genes (RNA)                       :: 58
            tRNAs                             :: 55
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4308
                     /organism="Prosthecobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7187_length_4308_cov_22.675053"
                     /isolate="AMR_MDS_5629"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1965333"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..502
                     /locus_tag="PLP58_20970"
     CDS             <1..502
                     /locus_tag="PLP58_20970"
                     /inference="COORDINATES: protein motif:HMM:NF024767.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HRJ11066.1"
                     /translation="DIADCMRFLADALGAAGRHDEAVQTARDALALAVEDGNGGRISS
                     AQVMLAVTLERARRLDEAHAAHAAAFAGHSDRNASFKDRVYDLHAMAGIRRSQGRHDE
                     ALAHHQEAWRLTLADPASKADPDFFPFLARPAMQCWKERLANDPDAAPPPELQEWEAW
                     LNRPKP"
     gene            complement(567..1718)
                     /locus_tag="PLP58_20975"
     CDS             complement(567..1718)
                     /locus_tag="PLP58_20975"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007413400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HRJ11067.1"
                     /translation="MTPPPIRTPGRQCEVAWFSALCSDDYEFLGVPDGSLRSSYEHCG
                     DIVRKADELGFQNILLPSGWIAGQDALAFAAAMAPQTKQINQLVALRMGEVWPPMLAR
                     ALATVDHIAIGRLCINIISSDMPGIKESNETRYARASEIIQILQALWRTDGPFEWKGE
                     FYQISLPTTEPAKPYQQNGGPLMYFGGISPAAQDLCARHCDVFLMWPETEDRIAETMR
                     LMSEKAAGYGRQIDFGFRGHIIVRETEAEARAAADRLISKLTAEKGAEIKARSQDSQS
                     FGVQRQDELRAQSKDLYIEPHLWSGIGLARSGCASAIVGDPDQVLAKLNRYMDMGIRA
                     FILSGYPHLDECDLFAKHVLTKLPTCRLNELQGRRVPDPVTPLTTAERK"
     gene            complement(1730..2452)
                     /locus_tag="PLP58_20980"
     CDS             complement(1730..2452)
                     /locus_tag="PLP58_20980"
                     /inference="COORDINATES: protein motif:HMM:NF018178.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1080 domain-containing protein"
                     /protein_id="HRJ11068.1"
                     /translation="MTRRHIFNLLSLALTSRVMGAEAVEWQAIFDGRTLQGWKPTAKS
                     PHSKASKNTTAGNWRVEDGVICGDQDTPGNGGLLITEAVYGDFEVTVETNNDFGPDSG
                     LFLRCTEDGKAYQCLIDYHPGGTIGGILGEGIWKRRGVRNYTFGGTPDVITLNENPQQ
                     PCPVSPEAWKTFWRVGGWNEVRARITGDPPTITTWVNGVQIMRHTEPASVHPATGHIG
                     LQIHGGKFSGTVRYRNIRIRQL"
     gene            complement(2606..>4308)
                     /locus_tag="PLP58_20985"
     CDS             complement(2606..>4308)
                     /locus_tag="PLP58_20985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ11069.1"
                     /translation="AREAAARLLRERPAEARTWMIQARTLTEPGEFEEKIAALDKAIE
                     ISPDEPRPADLKALALTQAGLYDAALEVCRAHPSADVSLRMRESWILWERGGKDEAVR
                     VMEAALARDPAHIWGWERLADWHQERQELDKAEAACRQLIRLQPMEHVPLGYLGSLLE
                     ERDKKQEAAAAYTRALEIFPYYPFAIARLLGLRFAAREWPEAAGLLESLTPHHPALFL
                     DSWRFALERRRGGHQQALVILEHMLGDSHDDESCFQRVLDEVKAAPNSLRRAVCETSA
                     RLLKEGCPQIHAGAFYVGVCKICEAAPDLQLLEKLPPGTQAGQRAWNAWINLQGDTWV
                     QNTAVWARLRQRRRWRAVMARHADAFRAESRLYGSVGYTLRNSSQNRALIEWFADWRE
                     RGPGMRPYMLNNLVLACQELGDAATAAEVVRAGMAYPEHGQLKMRFHLWAALEHALNE
                     ETAAALDCLQSVNPHVLDGYGQSLHGFVQTALEYQPGRRPPPFDHTRKNQLGTLFDEH
                     RNNRLIRASIRRCCRLASRRLGTSKPARWAFWKDNERFVGHAWLVFAILCFAVALSIR
                     "
BASE COUNT          728 a         1260 c         1495 g          825 t
ORIGIN      
        1 ggacatcgcc gactgcatgc gcttcctggc ggacgcgctc ggcgccgccg gccgtcatga
       61 cgaggcggtg caaaccgcgc gcgacgccct ggcgctcgcc gttgaggacg gcaatggcgg
      121 gcgcatttca agcgcccagg tgatgctggc cgtcacgctg gagcgcgccc ggcgtctcga
      181 tgaggcgcat gccgcccatg ccgccgcttt tgccggtcac agcgaccgca acgccagctt
      241 caaagaccgc gtttacgatc tccacgccat ggcgggcatt cgccgaagtc agggccgtca
      301 tgacgaggct ctggcgcatc atcaggaggc atggcgcctg actctggcgg atcccgcctc
      361 caaagcggat ccggactttt tccccttcct ggcccgcccg gctatgcagt gctggaaaga
      421 gcggcttgcc aatgacccgg atgccgcgcc tccaccggaa ctccaggaat gggaggcatg
      481 gctcaaccgc ccaaagccgt gacgacacgg aaggattctg ccgcgggcgc cctgcgggct
      541 cgcaggatcg gcgcggagga tgtttgtcat tttctctccg ccgtggtgag tggtgtgaca
      601 ggatcgggaa cgcggcggcc ttggagttcg ttcaagcggc aggtggggag tttggtgagg
      661 acatgtttgg cgaagaggtc gcactcgtcg aggtgcggat aaccgctgag gatgaaggca
      721 cggatgccca tgtccatata ccggttcagt ttggcaagga cttgatcagg atcgcccaca
      781 atggcgctgg cgcagccgct gcgggcgaga ccgatgccac tccataaatg cggctcgatg
      841 taaagatcct tcgattgcgc gcggagctcg tcctggcgct gcactccgaa gctctggctg
      901 tcctgggagc gcgccttgat ttccgccccc ttttcggcgg tgagtttgga aatgaggcgg
      961 tcggctgcgg cgcgggcctc ggcctccgtt tcgcgcacga tgatgtgccc acgaaagcca
     1021 aaatcaatct ggcggccgta gccggcggct ttttccgaca tgaggcgcat ggtctcggcg
     1081 atgcggtcct ccgtctccgg ccacatgagg aagacatcgc agtgcctggc gcacaaatcc
     1141 tgcgcggcgg gcgagatgcc gccgaagtac atgagcgggc cgccgttttg ctggtagggc
     1201 ttcgcaggtt cggtggtggg aaggctgatt tgataaaact ctcccttcca ctcgaaagga
     1261 ccgtcggtac gccagagcgc ctgcaggatc tggatgatct cgctggcgcg ggcgtagcgc
     1321 gtctcattgc tctccttgat gccgggcatg tcggaggaga tgatgttgat gcacaggcgg
     1381 cctatggcga tgtgatcgac ggtggcgagg gcgcgcgcga gcatcggcgg ccaaacctcg
     1441 cccatgcgca gggcgacgag ctggttgatc tgcttggttt gcggtgccat ggcggcggcg
     1501 aaagccagcg cgtcctgccc ggcgatccag ccgctgggca gcaggatgtt ttgaaagccg
     1561 agttcgtccg cttttcgcac gatgtcaccg cagtgctcgt agctggagcg cagggaacca
     1621 tcggggacgc cgaggaactc gtagtcatca gagcaaagag cggaaaacca ggcgacttcg
     1681 cattggcgac cgggcgtgcg gatgggggga ggtgtcatga tggtgtgact caaagctggc
     1741 ggatgcggat gttgcgatag cgcacggtgc cggagaattt gccgccgtgg atttgaagac
     1801 cgatgtgacc ggtggcggga tgcacgctgg caggctcggt gtgccgcatg atctgcacgc
     1861 cgttcaccca agtggtgatg gtgggcggat cgccggtgat gcgggcgcgg acctcattcc
     1921 atccgccgac acgccagaag gtcttccatg cctctggtga gaccgggcag ggctgctgcg
     1981 ggttctcatt gagcgtgatg acgtccggcg taccgccaaa ggtgtagttg cgcacaccgc
     2041 ggcgcttcca gatgccttcg ccaaggatgc caccgatggt gccgcccggg tggtagtcga
     2101 tgaggcactg gtaggctttg ccatcctccg tgcagcgaag aaacaggccg ctgtcggggc
     2161 cgaaatcgtt gttggtctcg acggttactt caaaatcgcc atacacagcc tcggtgatga
     2221 gcaggccgcc atttccgggc gtgtcctgat cgccgcagat gacgccgtct tcgacccgcc
     2281 agtttccggc ggtcgtgttc ttgctggcct tgctgtgcgg gcttttcgcc gtgggtttcc
     2341 atccttgcag cgtgcgcccg tcgaagatgg cctgccattc aaccgcctcc gcgcccatga
     2401 cccggcttgt cagcgcgagg gacaggagat tgaaaatgtg ccgccgggtc atgaaggtaa
     2461 attgtatggg tttaattatt tctaaaccgg aatatgtccc gatttttcat gctgtggcaa
     2521 tctgtttcag aaatgtcatc cgactacctg ccgcagtatg gatgaggagt gcgtggtgac
     2581 aggccggacg tcgggcggtc tcaagtcagc gtatgctgag tgccaccgca aagcaaagaa
     2641 tggcaaacac cagccaggcg tggcccacaa agcgctcgtt gtccttccag aaagcccagc
     2701 gggctggttt tgaagtgcct aggcggcggg aggcgaggcg gcagcagcgg cggatggagg
     2761 cgcggatgag gcggttgttt cgatgctcgt caaagagggt gccgagctgg ttcttgcggg
     2821 tgtggtcgaa gggcggcgga cggcggccgg gctggtattc gagcgcggtt tggacaaagc
     2881 cgtggaggct ctggccatag ccgtcgagga catgaggatt gacgctttgc aggcagtcca
     2941 gagcggcggc ggtttcctcg ttgagggcgt gctccagggc ggcccaaagg tggaagcgca
     3001 ttttgagctg gccatgctcc gggtaggcca tgcccgcgcg caccacttcg gcggcggttg
     3061 ccgcatcacc cagttcctgg caggcgagca cgaggttgtt gagcatgtaa gggcgcatgc
     3121 ccgggccgcg ctcccgccag tcggcgaacc attcgatgag ggcgcgattt tggctggagt
     3181 tccttagtgt gtagccgacc gaaccataca gcctggactc ggcgcgaaag gcatccgcat
     3241 ggcgggccat gacagcgcgc catcggcggc gctggcgcag gcgcgcccag acggcggtgt
     3301 tttgcaccca ggtgtctccc tgcaagttga tccaggcatt ccaggcgcgc tggccggcct
     3361 gggtgccggg tgggagcttc tccagaagct ggaggtccgg ggccgcctcg cagattttgc
     3421 agactccgac ataaaatgcg cccgcatgta tttgaggaca gccttccttg agcaggcgcg
     3481 cgctggtttc gcacacggcg cggcgcaagg agttgggggc tgctttcacc tcgtcaagca
     3541 cgcgctgaaa gcagctctca tcatcatggg agtcccccag catgtgctcc aggatgacca
     3601 aagcctgctg atgaccgccg cggcggcgct caagggcgaa gcgccaggaa tcaagaaaca
     3661 aggccggatg atggggcgtc aagctttcca ggagtccggc agcctcaggc cattccctgg
     3721 cggcaaagcg caggccgagc aggcgtgcga tggcgaaggg gtagtagggg aatatttcca
     3781 gcgcgcgggt gtaggctgcg gcggcctcct gttttttgtc ccgttcttcg agcaggctgc
     3841 cgaggtagcc gaggggcaca tgctccatgg gctggagacg gatcaactgg cggcaggcag
     3901 cttccgcctt gtccagttcc tggcgttctt gatgccagtc cgcgagtcgc tcccaccccc
     3961 agatgtgtgc tgggtcccgg gccagggccg cctccatgac gcgcacggcc tcgtccttgc
     4021 cgcctcgctc ccagaggatc cagctctcgc gcatgcgcag cgagacatcg gctgaagggt
     4081 gtgcgcggca gacttccaga gcggcatcgt agaggccggc ttgcgtgagg gccagggctt
     4141 tgagatcggc cgggcggggt tcgtccgggc tgatctcgat ggctttgtcc agcgcggcga
     4201 tcttttcttc gaactcgccg ggttcggtga gcgtccgcgc ttgaatcatc caagtgcgcg
     4261 cctcggcggg gcgctcgcgc agcaggcgcg cggcggcctc gcgcgctg
//
LOCUS       DAOMYJ010000322         4301 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Prosthecobacter sp. isolate AMR_MDS_5629
            NODE_7199_length_4301_cov_18.959962, whole genome shotgun sequence.
ACCESSION   DAOMYJ010000322 DAOMYJ010000000
VERSION     DAOMYJ010000322.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867850
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prosthecobacter sp. (wastewater metagenome)
  ORGANISM  Prosthecobacter sp.
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Verrucomicrobiaceae; Prosthecobacter.
REFERENCE   1  (bases 1 to 4301)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4301)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.30
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:34:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,742
            CDSs (total)                      :: 4,684
            Genes (coding)                    :: 4,676
            CDSs (with protein)               :: 4,676
            Genes (RNA)                       :: 58
            tRNAs                             :: 55
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4301
                     /organism="Prosthecobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7199_length_4301_cov_18.959962"
                     /isolate="AMR_MDS_5629"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1965333"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            541..762
                     /locus_tag="PLP58_20990"
     CDS             541..762
                     /locus_tag="PLP58_20990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ11070.1"
                     /translation="MKTPAASTTRCTQGLTSGGVFTSLGSFMKNLASRELPPLTQSGP
                     IHPQKIWTNLICPHFMAAKQCSPNSTRHP"
     gene            1398..2171
                     /locus_tag="PLP58_20995"
     CDS             1398..2171
                     /locus_tag="PLP58_20995"
                     /inference="COORDINATES: protein motif:HMM:NF025464.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide lyase"
                     /protein_id="HRJ11071.1"
                     /translation="MHTMKPACLFFCFLTAAASAHAQTGLLKVADFESGDLSQLFTQK
                     AQTDSLTIVTLPVRAGQHAARTLLRASDPEVNGGQRAEFSDGKKLTRIEMEKDYWYGL
                     SIFLPREFVAPTKTNAVLFQWHTQQGGPSPVLAIRVLDGDWLITSNATEKKRLLARMP
                     FEKDRWTDWVVHVRWSAERKGFWTIWKDGVEVVNERDIVTQYPETLGPYAKFGQYHSV
                     GEVPQNVVYFDEYRVAGPDGSYAAVAPPNGHAPTGKGAR"
     gene            complement(2525..3691)
                     /locus_tag="PLP58_21000"
     CDS             complement(2525..3691)
                     /locus_tag="PLP58_21000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009965893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HRJ11072.1"
                     /translation="MRSAFLDKLLKRMDRLEPGEVQSVMLELMKEKGFLEKTFQALQE
                     GVILLDPEARVTYVNQAACQFFGLHAEQVIGQKLTQGMRGLDWGELLKPGAVVSRDLE
                     IFYPENRYLNFYITSIDDEQPMGFVMLIRDVTETRKRTEAQIESERLNAFTLLAAGVA
                     HELGNPLNSLTIHLQLIERRLKKMGKSGEQLREHLDVASAEIKRMDGIISQFLAAIRP
                     TQPQMRRASVAELLQESLRFLQPEIGQAKVKVRLDLRPDLPPMPLDAGQMKQAFYNLI
                     RNACQAMPEGGTLTIHGSFTDFEVRLSFEDTGRGISPEQMGRLFQPFSTTRPTGTGLG
                     LLIVRRIIREHGGEIDIESRAGLGTKVSLWLPLVERKVRLLEPGESRTGTDCPP"
     gene            3783..4133
                     /gene="cutA"
                     /locus_tag="PLP58_21005"
     CDS             3783..4133
                     /gene="cutA"
                     /locus_tag="PLP58_21005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004201171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010038 - response to metal ion [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="divalent-cation tolerance protein CutA"
                     /protein_id="HRJ11073.1"
                     /translation="MTDLLLVLTTFPDAAHARQAAAALVEERLAACASLVPGIESIYR
                     WQGAVETAAEVLLLIKTRAGLYDRLAGRLRGLHPYEVPEILALRPEHASPAYRQWLLA
                     ETGPSNGEPGKLGA"
BASE COUNT          714 a         1302 c         1418 g          867 t
ORIGIN      
        1 cgcggaggca gggcaggctg ccggggtggc ggaaaggtct ttggcgcggg ttcgggaact
       61 gctcctggac gggtggcggg gtttttcggc tcatccggga agttggtgcg gagtttgggt
      121 tccgcgcggc agcaccttca cggcggcagc ccttggactg cggcagcctg ctgccgcttt
      181 cggcaagcca gcctgctggc cgtcgagcgt tgccgtgcag tcttttcttt tggatggatc
      241 cccctgcgcc agtcctgggc gttgcgcgcg ccagcagaca gaggcggcag caggctgcct
      301 cggaaaagcg gcagcaggct gccgcagtcc agggtgcgga gcacttcaaa acccgtttgg
      361 aaaccgctcc gtgccgctgc tgctgctgca aattccgcgc caacttcccg gatgaaccgg
      421 ctttcccgcc tggccggggt tcatggttca cggcgtgtct ttgcagtgtt cagagcacgc
      481 tgaagcgcgg acgacaggcc cggaggcgcc atccgccgct ccctgtccgc attggctggc
      541 atgaaaacac ccgctgcctc gaccacccgg tgcacccaag gcttgacttc cggcggcgtt
      601 tttacttcgc tcggctcatt catgaaaaac cttgcttctc gtgaattgcc cccattgact
      661 caatccggcc cgattcatcc gcaaaaaatc tggacgaatc tgatttgccc acattttatg
      721 gcggctaaac aatgttcgcc taactcaaca cgccatccgt gatgccacct gaccagcagc
      781 ccatctcttt tcgctgccga ccatctccga aggtgatgtt attttgctgc ctattcttcg
      841 gagcttgtgc tgcggtgctc agccacaagg catccacgaa cacgagaggc cttattctga
      901 acggcatttt tgagttcagc ccttccagtg cctcggtatt ctactgggtt ttggctgctc
      961 tctccgtctt gtttgttgta gctggcggct tgacgatcct caccactttg gtccacggtg
     1021 tgcctgacgt agttttggca gcggactcca tttcatttcc cgtgggcttt ccgatcaagc
     1081 gtccatttag cctgccctac tctcagatca cgggattgag tcggagtgag gttagcggcc
     1141 agcgattttt gatgcttcac acagccacga agaagcatca catagtcttg aattggctgg
     1201 gatcgaaggt tgcagaggag agtttaccac aggaattagc ccagcgtcta accgagggca
     1261 cttcacagac atgactgcca cgcacacagc cggaaagaag gcgaaccagg cgtgggagac
     1321 aacggcacga aggctatctg tcgtgtcagc aacgtcttgc gctcgccgtc gccacagctt
     1381 agacgttgcg cttctgcatg cacaccatga aacctgcctg tcttttcttc tgtttcctta
     1441 ccgctgcggc ctccgcacac gcgcaaaccg gcttgctcaa ggttgcggat ttcgagtcgg
     1501 gcgatctctc gcaactgttc acgcaaaagg cacagaccga ttccttgacc atcgtgactt
     1561 tgcccgtgcg cgcgggtcaa catgcggccc gcacactcct gcgcgccagc gatcccgagg
     1621 tgaatggcgg tcagcgcgcg gaattttccg atggcaagaa gctcaccagg atcgaaatgg
     1681 aaaaggacta ctggtatggg ctgagcatct tcctgccccg cgagttcgtc gctcccacga
     1741 agacaaacgc ggtgcttttc caatggcaca cgcagcaggg cggaccgagt ccggtgctgg
     1801 cgatccgggt gctcgacggt gactggctca tcaccagtaa cgcgacggaa aagaagcgcc
     1861 tgctcgctcg aatgcctttc gagaaggatc gctggaccga ctgggtggtg catgtgcgct
     1921 ggtcggcgga gcgcaagggc ttctggacga tctggaagga tggtgttgaa gtggtgaacg
     1981 agcgcgacat cgtcacgcaa tatcccgaaa cgctcgggcc gtatgcgaag tttggacagt
     2041 atcacagcgt cggcgaggtg ccgcagaacg tcgtttattt tgacgaatac cgcgtggctg
     2101 gcccggacgg aagctatgca gcggtggctc cccccaatgg ccatgcacca acaggcaaag
     2161 gggcacgatg atctcctcat ttccattgcc aacgcgcctc ctgagtaact aagcttcgag
     2221 ttacgaggcg gggacgagct ggaggccggg ttgggcggct tgtttttgaa ggttggtgag
     2281 gcggaggggg tgactttgaa cgcctcgttt tcgtcgcagg ctttgccgct ctggatcatg
     2341 ccgtggatga tgcgggcggg gttgcttctc gcgccgcccc acggcgctcg cctctgcgtg
     2401 gcgcctgccg gctggctgtc tcgctccgct cggctgtggg cggtggcgac gatgcccccg
     2461 gccttgctcc tggccacagg gacggcattg cccgcgtctc cgctgacggt ctgaaccggc
     2521 gcagtcaggg ggggcagtcc gtcccggtcc gcgactcgcc gggctcgagc aggcggactt
     2581 tgcgctcgac gagggggagc cagaggctga ctttggtgcc gagaccggcg cgggattcga
     2641 tgtctatctc gccgccgtgc tcgcggatga tgcggcggac gatgagcagg ccgaggccgg
     2701 tgccggtggg gcgtgtggtg gagaagggct ggaagaggcg gcccatctgc tcgggggaga
     2761 tgcctctgcc ggtgtcctca aagctgaggc gcacttcgaa gtcggtgaag ctgccgtgga
     2821 tggtgagggt gccgccttcg ggcatggcct ggcaggcgtt gcggatgagg ttgtaaaagg
     2881 cctgcttcat ctggcctgca tccaggggca tgggcgggag gtcgggccgc aagtcgagcc
     2941 gcactttgac tttggcctgc ccgatctcgg gctggaggaa gcggaggctc tcctggagca
     3001 gctcggccac gctggcgcgc cgcatctgcg gctgggtggg gcggatggcg gcgaggaact
     3061 ggctgatgat gccgtccatg cgcttgatct cggcgctggc cacgtcgagg tgctcgcgca
     3121 gttgttcgcc ggacttgccc atttttttca gccgccgctc gatgagctgg aggtggatgg
     3181 tgagggagtt gagcgggttg cccagctcgt gggcgacgcc ggcggccagg agggtgaagg
     3241 cgttgagccg ctcgctctcg atctgcgcct cggtgcgctt gcgcgtctcg gtgacatcgc
     3301 ggatgagcat gacgaagccc atgggctgct cgtcgtctat gctggtgatg tagaagttca
     3361 ggtagcggtt ttcggggtag aagatctcca ggtcgcggct gaccacggcg ccgggcttga
     3421 gcaactcacc ccagtccagc ccgcgcatgc cctgagtgag cttctggccg atgacctgct
     3481 ccgcgtgcag gccgaagaac tggcatgcgg cctggttcac ataggtgacg cgggcctcgg
     3541 ggtcgagcag gatgacgccc tcctggaggg cctggaaggt cttttccagg aagccttttt
     3601 ccttcatcag ctcgagcatg acgctctgca cctcgccggg ttccaggcgg tccatgcgct
     3661 tgagcagctt gtccaaaaag gccgatctca tgggggtgtc ttgggtgttg gggaaaaagc
     3721 cgccggatca tgccatgcgc gcgccgcttt gcatccgcga aaagcgcccg tagtctgccg
     3781 ccatgaccga tctgctgctc gtcctcacca cgtttcccga tgccgcccat gcccgccaag
     3841 ccgccgccgc gctcgtggag gagcgtctgg ccgcgtgcgc cagcctggtg cccggcatcg
     3901 agtccatcta ccgctggcag ggcgcggtgg agactgcggc ggaggtgctg ctgctgatca
     3961 aaacccgcgc cggcctttac gaccggctcg ccggccgcct gcgcgggctg cacccctacg
     4021 aggtgccgga gatcctggcc ctgcgccccg agcacgccag cccggcctac cgccaatggc
     4081 tgctggcgga gacagggcct tcgaatgggg aacccggcaa acttggcgcg tgaaaacccg
     4141 cgccgcccgg cgttgttctc aggcccatga cccgcgcacc ccgcaccgaa gacgaactcg
     4201 aagaatggct cagccgcccc acgcccgccg cggcagacgc gctgcgcggg ctggaggggg
     4261 atgtgatggt gctcggcgcg ggcggcaaga tggggccgac c
//
LOCUS       DAOMYJ010000323         4284 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Prosthecobacter sp. isolate AMR_MDS_5629
            NODE_7249_length_4284_cov_19.032159, whole genome shotgun sequence.
ACCESSION   DAOMYJ010000323 DAOMYJ010000000
VERSION     DAOMYJ010000323.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867850
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prosthecobacter sp. (wastewater metagenome)
  ORGANISM  Prosthecobacter sp.
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Verrucomicrobiaceae; Prosthecobacter.
REFERENCE   1  (bases 1 to 4284)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4284)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.30
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:34:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,742
            CDSs (total)                      :: 4,684
            Genes (coding)                    :: 4,676
            CDSs (with protein)               :: 4,676
            Genes (RNA)                       :: 58
            tRNAs                             :: 55
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4284
                     /organism="Prosthecobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7249_length_4284_cov_19.032159"
                     /isolate="AMR_MDS_5629"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1965333"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..192)
                     /locus_tag="PLP58_21010"
     CDS             complement(<1..192)
                     /locus_tag="PLP58_21010"
                     /inference="COORDINATES: protein motif:HMM:NF013308.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NTP transferase domain-containing protein"
                     /protein_id="HRJ11074.1"
                     /translation="MTTAIIVAAGSSRRMGFDKLLAPLAGVPVLRRSITAFQDCADVG
                     DILVVASEAATAAVAAWKAQ"
     gene            complement(189..1658)
                     /locus_tag="PLP58_21015"
     CDS             complement(189..1658)
                     /locus_tag="PLP58_21015"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase"
                     /protein_id="HRJ11075.1"
                     /translation="MLRLLCCLALSAGSASAKTNVLLICVDDLKPALGCYGDSFAKTP
                     HTDRLARRGVLFEAAYCNQAVCSPSRNALMTGLRPQTLGIYELSTNFRKAAPDAVTLA
                     QHFRQQGYRTEAMGKIFHRGHGNIEDAASWTIPHWTPKAPTYALPESSANMREGRNGP
                     RGPATESAPVADDTYADGQTALEAVKRLKAAAQKPDEPFFIAVGFIRPHLAFVAPQKY
                     WDLYDSAAIPMPQVTEPPAGAPAYAGQFGGELRQYSDMPPQGDIDPAATRRLIHGYYA
                     ATSYMDAQLGLVLDALDQSGLAESTHIVLWGDHGWHLGDHRFWCKHTNYEQAARIPLI
                     LAGPGITAGAKTRALVETVDIYPTLTELAGLPAREGLDGLSFAPVLKDPAQAARQSVI
                     HVYPRNNLLGRAIRTARHRMVEWKKPGSPADTAEFELYDYQADPLETKNLAAEQPEVL
                     AGLRAILATHPEARPQIKSAASDTPAKKTGAGKKKKKQA"
     gene            1787..3223
                     /locus_tag="PLP58_21020"
     CDS             1787..3223
                     /locus_tag="PLP58_21020"
                     /inference="COORDINATES: protein motif:HMM:NF016612.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="neutral/alkaline non-lysosomal ceramidase
                     N-terminal domain-containing protein"
                     /protein_id="HRJ11076.1"
                     /translation="MIKTASLLLLLSATLATRAEFRAGAYAQDISPVKFPAPVNGSMS
                     ANFASGIHDPMHARCLALHDGKRALVFVVVDACLIPREVCEEAKDIASKETGIPKEHM
                     LISATHTHSAAALTPVFQSETDPDYVRTVPPLIARGIAQAVKNLEPAELGWAFGSDPS
                     QVFNRRWHMKPGQYYENPFGITTDRARMNPGNVSRDVSVPTAPVDQDVAVLAVRSAAD
                     QRPLGLLANYSLHYVGGNPAISADYFGAFAAEVGRMLNAGDARYADKPAFVGIMSNGT
                     SGDVNNANYGLSIRHKRNPGEQIQIVARSVATAALEAYQAIAWKKDVTLDSEETELHL
                     GVRKASAQELAQAREWMQTIPKAANGQWADRKAIYAREAVLLADYPDTVPVKLQMHRI
                     NDLSVGAIPCEVFVEIGLRLKRTSPFTRHFTISLANGYNGYLPTGEAHAMGGYETWRA
                     RSSYLEVPAAGKITEALEQMAATLERRR"
     gene            3649..>4284
                     /locus_tag="PLP58_21025"
     CDS             3649..>4284
                     /locus_tag="PLP58_21025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ11077.1"
                     /translation="MSASFAAPPLNASSSPGHGAPLFKAVPGESFQASPFNPAPATAS
                     VAPLTVGDLLPQLPPDVARVTGLPPDHPVALPQEVLENALSSGKPEVPIFEVWRVCPA
                     MFQTTISPQDPRLVPLPAAKLPRLIAITQPGQGAQPFSEPASAPAPAFTPAASPFTAA
                     PASPFTAQAAASAPAVFGRENAPPASPAGIALPPRRNGPPQRSCSGFSRRRP"
BASE COUNT          694 a         1359 c         1477 g          754 t
ORIGIN      
        1 ctgggccttc catgccgcca cggcggcggt ggcggcctcg gaggcgacga cgaggatgtc
       61 tcccacgtcg gcgcagtcct gaaaggcggt gatggagcgg cgcagcacgg gcacgccggc
      121 caggggggcg agcagtttgt caaaccccat gcggcggctg ctgccggcgg cgacgatgat
      181 ggcggtggtc atgcctgctt tttcttcttt ttgccggcgc cggttttttt cgcgggggtg
      241 tcggaggcgg cggacttgat ctgcggcctg gcctcggggt gggtggcgag gatggcgcgc
      301 aatccggcaa gcacttcggg ctgctctgcg gcgaggtttt tcgtttcgag cgggtcggcc
      361 tggtagtcgt aaagctcgaa ctcggcggtg tcggcgggtg agccgggttt tttccactcg
      421 accatgcggt ggcgcgcggt gcggatggcg cggcctaaca gattgttgcg cggatagacg
      481 tggatgacgc tctggcgggc ggcctgggcg gggtccttca agacaggggc gaagctgagg
      541 ccgtccaacc cctcgcgcgc gggcagcccg gcgagttcgg tgagggtggg gtaaatgtcc
      601 acggtctcga cgagggcccg agtcttcgcg ccggcagtga tgcctgggcc ggcgaggatg
      661 agggggatgc gcgcggcctg ctcgtagttc gtgtgcttgc accagaagcg gtggtcgccg
      721 aggtgccagc cgtggtcgcc ccagaggacg atgtgggtgc tttcggcgag tccgctctgg
      781 tcgagcgcgt cgagcacgag gccgagctgg gcgtccatgt agctggtggc ggcatagtaa
      841 ccgtggatga ggcggcgggt ggcggcgggg tcaatgtcgc cctggggcgg catgtcggag
      901 tattggcgca gctcgccgcc gaactgcccg gcgtaggcgg gcgcgccggc gggcggctcg
      961 gtgacctggg gcatggggat ggcggcgctg tcgtagaggt cccagtattt ctgcggggcg
     1021 acgaaggcga ggtgcgggcg gatgaagccg acggcgatga agaagggctc gtcgggcttt
     1081 tgcgcggcgg ccttgaggcg cttcacagct tcgagggcgg tctggccgtc ggcataggtg
     1141 tcatcggcca caggggcgct ttccgtggcg gggccgcgcg ggccgttgcg gccctcgcgc
     1201 atgttggcgc tgctttcggg gagggcgtaa gtgggggctt tgggagtcca atgcgggatg
     1261 gtccaggagg cggcgtcctc gatgttgccg tggccgcggt ggaagatctt gcccatggcc
     1321 tcggtgcggt agccctgctg gcggaagtgc tgcgccaggg tgacggcatc cggcgcggct
     1381 ttgcggaagt tcgtgctcag ctcgtagatg ccgagggttt gcgggcgcag gccggtcatg
     1441 agcgcgttgc gggaggggga gcagacggct tggttgcaat acgcggcctc gaagagcacg
     1501 ccgcgcctgg cgaggcggtc ggtgtggggc gttttggcga agctgtcgcc atagcagccg
     1561 agggcgggct tcagatcgtc cacgcagatg agcaggacgt tcgttttggc ggacgcggag
     1621 ccggcgctga gggccaggca gcaaaggagg cggagcattt tcatgaggcc gtccgtaacg
     1681 cggcgcgcgc ggcctttcat ctggaaaaag ccgcggcacc tggcgtcaac atccagttac
     1741 caagtccgcc aagaaccgcc gctgcggcgg cgtatgcctc cgcgccatga tcaagaccgc
     1801 ctccctgctc ctgctgctct ccgccaccct cgccacccgc gctgaattcc gcgcgggggc
     1861 ctacgcccag gacatctcgc ccgtgaaatt ccccgcgccg gtgaacggca gcatgagcgc
     1921 gaactttgcc agcggcatcc acgaccccat gcacgcgcgc tgcctggcgc tgcacgacgg
     1981 caaacgcgcg ctcgtctttg tcgtggtgga tgcctgcctc atcccgcgcg aggtctgcga
     2041 ggaggcgaag gacatcgcct cgaaggagac cggcattccg aaagagcaca tgctcatctc
     2101 cgccacgcac acgcattccg ccgccgcttt gacgcccgtc ttccagagtg aaactgaccc
     2161 cgattatgta aggacggtgc cgccgctcat cgccaggggc atcgcgcagg cggtgaaaaa
     2221 cctggagcct gccgagctgg gctgggcctt tggcagcgac cccagccagg tcttcaaccg
     2281 tcgctggcac atgaagccgg gccagtatta tgagaacccc ttcggcatca ccaccgaccg
     2341 cgcgcgcatg aatcctggca acgtcagccg ggatgtgtcc gtgcccaccg cgccggtgga
     2401 tcaggacgtg gccgtgctgg ccgtgcgctc cgccgccgat cagagaccgc tgggcctgct
     2461 ggcgaattac agcctccact atgtgggcgg gaaccctgcc atcagcgcgg actatttcgg
     2521 cgctttcgcc gccgaggtcg ggcgcatgct caacgcgggc gacgcgcgct acgcggacaa
     2581 gcccgccttt gtcggcatca tgtccaacgg caccagcggc gacgtgaaca acgccaacta
     2641 cggtctctcc atccgccaca agcgcaatcc tggagagcaa atccagatcg tggcgcgcag
     2701 cgtggcgacg gcggcattgg aggcgtatca agccatcgcc tggaaaaagg acgtgacgct
     2761 cgactccgag gagaccgagc tgcatctcgg tgtgcgcaaa gccagtgcgc aggagctggc
     2821 gcaggcgcgc gaatggatgc agacgatccc gaaagccgcc aacggccagt gggccgaccg
     2881 caaggccatt tacgcgcgcg aggccgtgct gctggcggat tacccggaca cggtgccggt
     2941 gaagctgcaa atgcaccgca tcaacgatct ctcggtgggc gccatcccgt gcgaggtctt
     3001 cgtggagatc ggcctgcggc tgaagcgcac ctctcccttc acccgccact tcaccatctc
     3061 cctggcgaat ggttacaacg gctacctgcc caccggggag gcgcacgcca tgggcggcta
     3121 tgagacctgg cgcgcgcgca gcagttacct ggaggtgcct gcggcgggaa aaatcaccga
     3181 ggctctggag cagatggcgg ccacgctgga gcggcggcgt taagcgcgcc tgcggatgcg
     3241 tggaagcggc gtcctgccgt tggcctttcc ccagcagccg gagcccggtt gcaaaaaaat
     3301 ccgcgcaaac agtgcgactt ggcttgaggc aaaaagcgtc gggatgccgc ttcaacttca
     3361 cacgtcacgt tcccttcttg aaagataggg gagaaagtga aaaaaatgtg gacttaagag
     3421 atgttaatta ttatcatctc gaaaatttcc attcggaaaa tggaattctt gtttttatag
     3481 tttcatgaaa tagtgttgat cgttgtttgt tcttagccgc cgctcgctcc catgggtatc
     3541 acactacgca gcatgtttcc cgcaggaccg gagaccaccg atccggcagc cggaaccatg
     3601 aacacccctt cggcggggcc ttttaccgct caatctcctt ttcaagcgat gtccgccagc
     3661 ttcgcagcgc cacctctgaa tgcctcctca tcaccaggcc atggcgctcc cttgttcaag
     3721 gcggtccctg gcgagtcttt tcaagcctcc cccttcaatc ccgcgcccgc cacagcaagc
     3781 gtggcgccgc tgactgtggg ggatctgctg ccgcaactgc cgccggatgt ggcgcgcgtc
     3841 accggcctgc cgcccgatca tccagtggcc ctgccgcagg aggtgcttga gaacgccttg
     3901 agcagcggaa agcccgaagt gccgatcttc gaagtctggc gggtctgccc ggcgatgttt
     3961 cagaccacca tctcgccgca ggatccgcgc ctcgtgcccc tgcctgcggc gaaacttccg
     4021 cgcctcatcg ccatcaccca gccgggccag ggcgcgcagc cattctcgga acccgcatcg
     4081 gcgcccgcgc cagccttcac gccggcggca tcgccgttca ccgcagcacc cgcttcgccc
     4141 ttcacagcgc aggcagccgc gtccgccccg gcagtctttg gccgggaaaa cgcgccgcct
     4201 gcatccccgg cgggcattgc cctgccgccg cgccgcaacg gccccccgca gcgctcgtgc
     4261 tctggttttt cgagacgcag gccc
//
LOCUS       DAOMYJ010000324         4262 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Prosthecobacter sp. isolate AMR_MDS_5629
            NODE_7310_length_4262_cov_21.920371, whole genome shotgun sequence.
ACCESSION   DAOMYJ010000324 DAOMYJ010000000
VERSION     DAOMYJ010000324.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867850
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prosthecobacter sp. (wastewater metagenome)
  ORGANISM  Prosthecobacter sp.
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Verrucomicrobiaceae; Prosthecobacter.
REFERENCE   1  (bases 1 to 4262)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4262)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.30
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:34:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,742
            CDSs (total)                      :: 4,684
            Genes (coding)                    :: 4,676
            CDSs (with protein)               :: 4,676
            Genes (RNA)                       :: 58
            tRNAs                             :: 55
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4262
                     /organism="Prosthecobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7310_length_4262_cov_21.920371"
                     /isolate="AMR_MDS_5629"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1965333"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            244..1506
                     /gene="murA"
                     /locus_tag="PLP58_21030"
     CDS             244..1506
                     /gene="murA"
                     /locus_tag="PLP58_21030"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009960849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008760 - UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HRJ11078.1"
                     /translation="MEKLIVHGGAPLKGRVNISGSKNSSLPILAATLLTRDTCTIRRV
                     PDLSDTNYMVRILGELGSEVERASGVVVVKAEKIKSVAPYDLVRKMRASICVLGPLTG
                     RLKKCTVSLPGGCVIGDRPVDLHLKGLEALGAQIEVEGGDIRVNARKGFKGGPMNLMG
                     KHGSTVLGTDNVMMAAVLAKGTTIIEGAAAEPEVEDLADFLVKMGAKIEGAGSNRIVI
                     QGVKELHGAEHTVIPDRIEAGTFLCAGAMTGEGITLRRVMPEHLTAVVDALKKCGFPV
                     ETTRDSITIAPNPGARGFDLTTLCYPGFPTDMQAQFCALACAINDTSTITETIFPQRF
                     MHVAEMKRMGADIDLQGATARIRGGAPLKGAPVMASDLRASAALVLAGLVAGGRTEVN
                     RLYHIDRGYEHLDDKLASLGAHVERVRE"
     gene            complement(1512..2186)
                     /locus_tag="PLP58_21035"
     CDS             complement(1512..2186)
                     /locus_tag="PLP58_21035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ11079.1"
                     /translation="MNDSLPRFWLRLLTFRATQDDYASLGPRHALAGLAACWVVGMGR
                     YWDDPRASLLQHLGAGSVVYVFVLSALLWCVVKPLEPLLFSYARVLAFITMTAPPAIL
                     YAVPVEKWMTLQEANHMNLRFLLLVAAWRVALWVHNLRVWGRFSPGTLVVAATMPLAV
                     IFWALTSLNLQHVVVNIMGGIREADKSSQDAAWSWLFTMTLLSVPVSVASALAWLLIL
                     ARDRNN"
     gene            complement(2179..3531)
                     /locus_tag="PLP58_21040"
     CDS             complement(2179..3531)
                     /locus_tag="PLP58_21040"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Presence of a B(12) (cobalamin)-binding domain
                     implies dependence on cobalamin itself, in one of its
                     several forms, or in some unusual lineages, dependence on
                     a cobalamin-like analog; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin-dependent protein"
                     /protein_id="HRJ11080.1"
                     /translation="MNPVNSRPQPRLLLLAMSGVRVCDEELLRLGLTLPGFVERSRVI
                     ASLPSLGLLTLAARTPGSWEIVYREYDEMPEDAARRIAEEEFTVVAISSLTARIEEAY
                     RLADDLRALRVLVIMGGLHVSAMPEEAAGHADIVVKGEGEMVWGEIIAGIEHQDWRAF
                     YDASKVAGDAHFGFPQPPPRYDLLDINRYNRIPLQTTRGCPLHCEFCAASRTISPWRS
                     KSIAQIGREMDAILALWPRPFVELADDNTFVDKKWSRELACLLAAHPVRWFTESDISL
                     ADDRELLRLLAKSGCAQVLIGLESAAQEAMRGLDTRDWKSARHASYRERVRVIQDHGI
                     SVNGCFVLGFDTDGPDIFQRTLNFIDMLDLAEVQITLLTAFPGTSLRRRLESEGRLLP
                     EAGWRHHTLFDVTFQPSRMTVDALRSGFRELMSGVYSAERVIRRGAIFKQCLKNRAHE
                     "
     gene            complement(3674..>4262)
                     /locus_tag="PLP58_21045"
     CDS             complement(3674..>4262)
                     /locus_tag="PLP58_21045"
                     /EC_number="4.1.1.31"
                     /inference="COORDINATES: protein motif:HMM:NF012532.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008964 - phosphoenolpyruvate carboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA];
                     GO_process: GO:0015977 - carbon fixation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxylase"
                     /protein_id="HRJ11081.1"
                     /translation="EHSRIGSRPSRRTGQATLDDLRAIPWVFSWTQSRFYLPGWFGTG
                     AALDKLRTEDSAAWDTLAAELRRSPFLRYVLTNIESSLVSANESLMRDYAALVPDGTV
                     RAHFLDMILAEHQRTRLMLEELFQGTFRQRRPRLAYTLEIREEPLAVLHAQQITLLRE
                     WRAHQEAGRAGDAEAMLPDLLISINALASGLRTTG"
BASE COUNT          835 a         1415 c         1350 g          662 t
ORIGIN      
        1 tgcgacgtat cgcagccgac gtgcgttccc gtcagcccga gcgtctcgcg caccagctcg
       61 acgagaagcg tccggtcctc gacctcggcc gaggcggcct tgccattgac ggtcagttcg
      121 acggcgggca tcccgcgctt cgtcctcgcc acagcgcccg cacgcgccgc tgaggctgcc
      181 gcgccggaga cggagtgatt ttccgcttgc agcggggaac gcgcggcgca cttcacaccg
      241 cacatggaaa aactcatcgt tcacggcggc gcgccattga agggccgcgt caacatcagc
      301 gggtcgaaga actcctccct gcccatcctc gccgccaccc tgctcacccg ggacacctgc
      361 accatccgcc gcgtgcccga cctgagcgac accaactaca tggtgcgcat cctcggcgag
      421 ctgggctcgg aggtcgagcg cgccagcggt gtcgtggtgg tcaaggcgga gaaaatcaag
      481 tccgtggcgc cctacgatct ggtgcgcaaa atgcgcgcca gcatctgcgt gctcgggccg
      541 ctgaccggga ggctgaaaaa atgcaccgtc tccctgcccg gcggctgcgt cattggcgac
      601 cggcccgtgg atttgcatct caaaggcctg gaggcgctgg gcgcgcaaat cgaggtggag
      661 ggcggggaca tccgtgtgaa cgcgaggaag ggcttcaaag gcggccccat gaacctgatg
      721 ggcaaacacg gctccaccgt gctcggcacg gacaatgtca tgatggccgc cgtgctcgcc
      781 aaaggcacga ccatcatcga aggcgccgcc gccgagccgg aggtcgagga cctggcggac
      841 ttcctcgtca agatgggcgc caaaatcgag ggcgccggca gcaaccgcat cgtcatccag
      901 ggcgtcaagg aactgcacgg cgcggagcac accgtcatcc cggaccgcat cgaagcgggc
      961 accttcctct gcgcgggcgc gatgacaggc gaaggcatca ccttgcgccg cgtcatgccg
     1021 gagcacctga ccgcagttgt tgacgctttg aaaaaatgcg gcttccccgt cgagaccacg
     1081 cgcgacagca tcaccatcgc cccgaatccc ggcgcgcgcg gctttgacct caccaccctc
     1141 tgctatccgg gctttcccac cgacatgcag gcgcagtttt gcgcgctggc ctgcgccatc
     1201 aacgacacca gcaccatcac cgagaccatc ttcccgcagc gcttcatgca tgtggcggag
     1261 atgaagcgca tgggcgccga cattgacctc cagggcgcca ccgcgcgcat ccgcggcggc
     1321 gctccgctca aaggcgcgcc cgtcatggcc agcgacctgc gcgcctccgc cgcgctcgtc
     1381 ctggccggac tcgtcgccgg cggcaggacc gaggtgaacc gcctctacca cattgaccgc
     1441 ggctacgagc acctcgacga caagctcgcc tccctcggcg cgcatgtcga gcgcgtgcgg
     1501 gagtgaggcg gtcagttgtt tctgtccctc gccaaaatca aaagccaggc cagcgccgaa
     1561 gccaccgaca ccggcactga cagaagggtc atggtgaaaa gccagctcca tgccgcgtcc
     1621 tgggaggact tgtccgcctc gcgtatgccg cccatgatgt tgaccacgac atgctggagg
     1681 ttcagcgagg tcagcgccca aaaaatgacg gccagcggca tggtggcggc cacgaccaga
     1741 gtgcccggcg aaaagcgtcc ccatacccgc agattgtgaa cccacaaagc cacacgccag
     1801 gcagccacca gcagcagaaa gcgcaggttc atgtgattgg cctcctgcag agtcatccac
     1861 ttctccaccg gcacggcata aaggatggca ggcggagccg tcatcgtgat gaaagccagc
     1921 acgcgcgcat aactgaaaag caggggttcc aaaggcttca ccacgcacca gagaagggcc
     1981 gacagcacaa aaacatagac cactgagccg gctcccagat gctgaagcag cgaagcgcgc
     2041 ggatcatccc aataccggcc catgcccacc acccagcagg ctgccaggcc ggcgagcgca
     2101 tggcgcggtc ccaggctcgc atagtcatcc tgcgtggcac ggaaagtgag gagcctcagc
     2161 cagaagcggg ggagggagtc attcatgagc gcggtttttc aggcattgtt taaaaatggc
     2221 gccgcgccgg atgacacgtt ccgcggaata aaccccgctc atcaattccc gaaagcccga
     2281 tctcaatgcg tccacagtca ttcgggaggg ttgaaatgtg acgtcaaaca aagtgtgatg
     2341 ccgccagccc gcctccggca gcaggcggcc ctcggactca agcctccggc gcaggctggt
     2401 gcctggaaag gcggtcagca gcgtgatctg aacctcggcc agatccagca tgtcaatgaa
     2461 gttgagcgtg cgctggaaaa tgtccggtcc atcggtgtca aaacccagca caaagcagcc
     2521 gttcaccgag atgccgtgat cctggatgac gcgaacgcgc tcgcggtagg aggcatggcg
     2581 cgctgatttc cagtcgcgcg tgtccaatcc gcgcatcgcc tcctgcgcgg ccgattccag
     2641 accgatgagc acctgggcgc agcccgattt tgccagcagg cgcaaaagct cgcggtcatc
     2701 cgcgagggaa atgtcactct cggtaaacca gcgcaccgga tgcgccgcaa gcaggcaggc
     2761 cagctctctc gaccattttt tgtccacaaa agtgttgtca tcggcaagct cgacaaaagg
     2821 gcggggccaa agggccagga tggcgtccat ctcgcgcccg atctgcgcga tgctcttact
     2881 cctccacggg ctgatggtgc gcgaagccgc gcaaaactcg cagtgcagcg ggcagcctcg
     2941 cgtcgtttgc aggggaatcc ggttgtagcg gttgatgtcc agcaggtcat accgtggcgg
     3001 cggctgtgga aagccaaaat gcgcgtcccc cgcgaccttg gaggcatcat aaaacgcccg
     3061 ccagtcctgg tgctcgatgc cggcgatgat ctcaccccag accatctccc cctcgccttt
     3121 gacaacgatg tccgcatggc ccgccgcctc ctccggcatg gcgctgacat gaagtccgcc
     3181 catgatgacc agcaccctga gggcgcgcag atcgtccgcc agtcggtaag cctcctcgat
     3241 acgagcggtc agggacgaga tggcgacaac ggtgaactcc tcctccgcga tgcggcgcgc
     3301 ggcatcttcg ggcatttcgt catattcccg gtaaactatc tcccaggagc ctggtgtgcg
     3361 ggctgcgagc gtcagcaggc cgagactggg cagcgacgca atgacgcgac tgcgttccac
     3421 gaaaccgggc agagtcagcc caaggcgcag cagttcctcg tcgcacacac gcacgccgct
     3481 catggcgagc agcaaaagtc ttggctgtgg cctggagttg acgggattca tttggaaaag
     3541 cggtcagacc ttgcgccggc aaagatgcag gccggccatg ctgacggtgg ctggaatttc
     3601 cacaagcacc cgcggagctt gcatctttca gcaagctgca cgccctggag gagcgccccg
     3661 gtgatggcgc gcctcacccc gtcgtccgca acccggaggc cagggcgttg atggaaatga
     3721 gcaggtctgg aagcatggcc tcggcgtcac cggcgcgccc tgcctcctgg tgcgcgcgcc
     3781 attcgcgcag gagggtgatc tgctgcgcgt gcagcacggc cagcggctcc tcgcggattt
     3841 ccagcgtgta ggccaggcgc gggcggcgct gccggaaagt gccttggaaa agctcctcca
     3901 gcatcagccg cgtgcgctgg tgctcggcga gaatcatgtc gagaaaatgc gcgcgcaccg
     3961 tgccgtccgg gacgagggcg gcatagtcgc gcatcaggct ctcattggcg ctgacgaggg
     4021 agctttcgat gttggtcagc acatagcgca ggaaggggga gcggcgcagc tccgcggcca
     4081 gggtgtccca cgcggcgctg tcctcggtgc gcagcttgtc caatgccgct ccggtgccga
     4141 accagcccgg caggtagaag cgcgactgcg tccatgagaa gacccacggg atggcgcgca
     4201 ggtcgtccag cgtggcctgg ccggtgcggc gggaggggcg cgagccgatg cgcgagtgct
     4261 cc
//
LOCUS       DAOMYJ010000325         4258 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Prosthecobacter sp. isolate AMR_MDS_5629
            NODE_7317_length_4258_cov_15.985724, whole genome shotgun sequence.
ACCESSION   DAOMYJ010000325 DAOMYJ010000000
VERSION     DAOMYJ010000325.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867850
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prosthecobacter sp. (wastewater metagenome)
  ORGANISM  Prosthecobacter sp.
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Verrucomicrobiaceae; Prosthecobacter.
REFERENCE   1  (bases 1 to 4258)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4258)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.30
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:34:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,742
            CDSs (total)                      :: 4,684
            Genes (coding)                    :: 4,676
            CDSs (with protein)               :: 4,676
            Genes (RNA)                       :: 58
            tRNAs                             :: 55
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4258
                     /organism="Prosthecobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7317_length_4258_cov_15.985724"
                     /isolate="AMR_MDS_5629"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1965333"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1960
                     /locus_tag="PLP58_21050"
     CDS             <1..1960
                     /locus_tag="PLP58_21050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ11082.1"
                     /translation="EWHREGRAAGLSAITYENRDEGHSRIEAGQYPQLQVFKPDEKSG
                     PPGGIAVMVRARPTVGNCSMSAPAERGGSLPRHYLMQPEGARFLMGQYLSNNLFIYPE
                     HQDHDPGANGAGGWGDLYPANTPCVIISQGSSLSDQPFVHAVLETIAAFPPETQKLLI
                     EKRLLMPTVQAIFRQSNKGVDTPQGYLTGAAHPPVFDAARLDVEKMARAANQMTPELV
                     PPVVQLEVVEENEERPGVHHFEDAKAAPLKLADTPVAVARVLHAGMGEKVMLVSAAKS
                     GDLTGRPLQIRWQVLQGDPALVRVETTDLNGTARVRVRWQPPVVNTAGVRSHRVDIGV
                     FALNGVTISAPAMLSFYMPPCEARFEDKDGRALEVLHQAHNPDFGLPAADTDPRWMRA
                     IFALSVAGDGLRSRLTEKLLGESERKALQQIWMSLDTRQREVRKLEADPARRTRAAEA
                     GARLGQEIALALREKLPGERALTARQAVERVLDAIAGFSDLYPSFQKELHRLAAQSPK
                     PGAEGVIRAEVARLVGLGVLYQEASGSVLTQSAPEKLSPGEQYCLRGLNLTLLSHALF
                     PEVLERSPAPAWTDPRISFPKPWRDVHRHDEEGRFLGWLRHHDGRTTWFSLDGKLLPD
                     GPGPSAPAHSVIYEAAPDGRMIWRRAER"
     gene            complement(2100..3368)
                     /gene="dgt"
                     /locus_tag="PLP58_21055"
     CDS             complement(2100..3368)
                     /gene="dgt"
                     /locus_tag="PLP58_21055"
                     /inference="COORDINATES: protein motif:HMM:TIGR01353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008832 - dGTPase activity [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dNTP triphosphohydrolase"
                     /protein_id="HRJ11083.1"
                     /translation="MPANTFYNSFDTETLDPRNTHPGEYRNAFQIDRDRIIHSSAFRR
                     LQNKTQVFLSGEYDFYRTRLTHSIEVAQIGRSICAWLNQQSGLLDEACHIDPDLVESA
                     CLAHDLGHPPFGHTGERTLHRLMQPYGGFEGNAQTLRILTQTLFNEGRDGMNPTRALL
                     DGVLKYKTLHAEAAGAKNHYLYSDQERWLDFTLGHQPFPAELTPGSARNSFRSIECQI
                     MDWADDTAYSLNDIADGIHAGFITVTRLEHWAAGQSLAPGQSAHLDFLCAAIRENRVE
                     ARLNRSIGDHIRAARLVPDASFLSAMTRRHQYRLETDPALAARAALNKKIALDLVFRS
                     PQLQQLDYKADFILTRMFEVLRARYIEPGGGSLHLMPAAQERQIENAPDTPARARLVC
                     DWIASMTDSFAFRTYRRLFDADFGSITDFV"
     gene            complement(3417..>4258)
                     /locus_tag="PLP58_21060"
     CDS             complement(3417..>4258)
                     /locus_tag="PLP58_21060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009964629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRJ11084.1"
                     /translation="PGPWQAAWRRLRQNRLAMAALAFLILLVAVCVAGPLFSPYGPND
                     QDLELKATAPSAAHWLGTDSLGRDLATRALHGGRVSLLVGIVATLVASVIGVGYGLVA
                     GLAGRRLDAFMMRLVDILYAFPFITFVIILVVVFGRELWLIFAAIGAVEWLTMARVVR
                     GQVLALKNLEFVTAARASGAGGAHILVRHMLPNVLGPVIIYASLTVPGVMLLEATLSF
                     LGLGIQPPDASWGVLIREGADSMETYPWMLAGPGLFFSATLLALNLLGDALRDALDPK
                     SGG"
BASE COUNT          641 a         1267 c         1710 g          640 t
ORIGIN      
        1 tgagtggcac cgggagggca gggcggcggg gctttcggcc atcacctatg agaaccggga
       61 cgagggccac tcgcgcatcg aggcggggca gtatccgcag ctgcaggtct tcaagccgga
      121 tgagaaaagc ggcccgccgg gcggcatcgc ggtgatggtg cgggcgcgcc cgacggtggg
      181 aaactgctcg atgtcggcgc cggcggagag gggcggcagc ctgccgcggc attacctgat
      241 gcagccggag ggggcgcgct tcctcatggg gcagtacctc tcgaacaatc tcttcatcta
      301 cccggagcac caggaccatg atccgggcgc gaacggggcg ggcggctggg gggacctcta
      361 cccggccaac acgccgtgtg tgatcatcag ccagggttcg tcgctctcgg accagccgtt
      421 tgtccacgcg gtgctggaga cgatcgcggc cttcccgccg gagacgcaga agctgctgat
      481 cgagaagcgg ctgctgatgc cgacggtgca ggccattttc cgccagtcga acaagggcgt
      541 ggacacgccg caggggtatc tgacgggcgc ggcgcacccg ccggtgttcg acgcggcgcg
      601 gctggatgtg gagaagatgg cgcgcgcggc caatcagatg acgccggagc tggtgccgcc
      661 ggtggtgcag ctggaggttg tggaggagaa cgaggagcgg cccggcgtgc accacttcga
      721 ggacgcgaag gccgcgccgc tcaagctggc ggacacgccg gtggcggtgg cgcgggtttt
      781 gcacgcgggc atgggggaga aggtgatgct ggtcagcgcg gcgaagtcgg gggacttgac
      841 gggccggccg ctgcaaatcc gctggcaggt gctgcagggg gacccggcgc tggtgcgcgt
      901 ggagacgacg gacctcaacg gcacggcgcg ggtgcgggtg cgctggcagc cgccggtggt
      961 caacacggcg ggggtgcgca gccaccgggt ggacatcggc gtcttcgcgc tcaacggggt
     1021 gacgatcagc gcgccggcga tgctgagctt ttacatgccg ccgtgcgagg cgcgcttcga
     1081 ggacaaggac ggccgcgccc tggaggtgct gcaccaggcg cacaacccgg acttcggcct
     1141 gcccgcggcg gacaccgacc cgcgctggat gcgcgccatc ttcgccctct ccgtggccgg
     1201 ggacgggctg cgctcgcgcc tgacggagaa gctgctcggc gagtccgagc gcaaggcgct
     1261 ccagcagatc tggatgagcc tggacacccg ccagcgggaa gtccgcaagc tggaggccga
     1321 ccccgcgcgc aggacccggg ccgcggaggc cggcgcgcgc ctcggccagg agatcgcgct
     1381 cgccctccgg gaaaagctgc ccggcgagcg cgccctcacc gcgcgccagg cggtggagcg
     1441 cgtgctggat gccatcgccg gtttcagcga cctctacccc tccttccaaa aggagctgca
     1501 ccgcctcgcc gcgcagtcgc ccaagccggg cgccgagggc gtgatccgcg cggaggtggc
     1561 ccgcctcgtt ggtctcggcg tgctttacca ggaggcctcc ggcagcgtcc tgacccagtc
     1621 ggcccccgaa aaactctccc ccggcgagca atactgcctg cgcggcctca acctcaccct
     1681 gctcagccac gcgctctttc ccgaggtcct ggagcgctcg cccgcgcccg cctggaccga
     1741 cccgcgcatc tccttcccca aaccctggcg cgatgtccac cgccacgacg aggagggccg
     1801 cttcctcggc tggctgcgcc accacgacgg ccgcacgacc tggttttccc tcgacggcaa
     1861 gctcctgccc gacggccccg gcccgtccgc cccggcccac tccgtcatct acgaagccgc
     1921 gccggatggc cgcatgatct ggcggcgagc ggagaggtga ggggcatggc gcgcgcgcat
     1981 cccagcttct gatcgcctca aaaccccgcc tccctgtgtg ccgcgcccgg atggcgctgg
     2041 cattgtttca cggatgctgc gcggcggcgg agacggcggg tgcaggacga gcggccggct
     2101 cagacgaagt cggtgatgga gccgaagtcg gcgtcgaaga ggcggcggta ggtgcggaag
     2161 gcgaagctgt cggtcatgct ggcgatccag tcgcagacga ggcgggcgcg cgcgggggtg
     2221 tcgggggcgt tctcgatctg ccgctcctgg gcggcgggca tgaggtggag gctgccgccg
     2281 ccgggctcga tgtagcgggc gcgcaggacc tcgaacatgc gggtgaggat gaagtcggcc
     2341 ttgtagtcga gctgctggag ttgggggctg cggaagacga ggtcgagggc gatctttttg
     2401 ttgagcgcgg cgcgggcggc gagggcgggg tcggtctcga ggcggtattg atggcgacgg
     2461 gtcatggcgc tgaggaagct ggcgtcgggg acgaggcggg cggcgcggat gtggtcgccg
     2521 atgctgcggt tgaggcgggc ttcgacgcgg ttttcgcgga tggcggcgca gaggaagtcg
     2581 aggtgcgcgc tttgcccggg ggcgaggctt tggccggcgg cccaatgctc gagcctggtg
     2641 acggtgatga agccggcgtg gatgccgtcg gcgatgtcgt tgagggagta ggcggtgtcg
     2701 tcggcccagt ccatgatctg gcattcgatg ctgcggaagc tgttgcgggc gctgccgggg
     2761 gtgagttcgg cggggaaggg ctggtggccg agggtgaaat ccagccagcg ctcctggtcg
     2821 ctgtagaggt agtggttttt ggcgcctgct gcctcggcgt ggagggtttt gtatttgagc
     2881 acgccatcga gcagggcgcg ggtggggttc atgccgtcgc ggccttcatt gaagagggtc
     2941 tgggtgagga tgcggagggt ctgggcgttg ccctcgaagc cgccgtaggg ctgcatgagg
     3001 cggtgcaggg tgcgctcgcc ggtgtggccg aagggtgggt ggccgaggtc gtgggcgagg
     3061 caggcgctct cgacgaggtc gggatcaatg tggcaggctt cgtcgagcag gccgctctgc
     3121 tggttgagcc aggcgcagat ggagcggccg atctgggcga cttcgatgct gtgggtgagg
     3181 cgggtgcggt agaagtcgta ttcgccggag aggaagacct gggttttgtt ttgcaggcgg
     3241 cggaaggcgc tgctgtggat gatgcggtcg cggtctatct ggaaggcgtt gcggtattcg
     3301 ccggggtggg tgttgcgcgg atcgagggtc tcggtgtcga agctgttgta gaaggtgttg
     3361 gcgggcatgg gcggggcctg gatcagagtt tgggggcggt ttttcgaaaa cgaaggtcac
     3421 ccgccggact tcgggtcgag cgcgtcgcgc agggcgtcgc cgaggaggtt gagggccagg
     3481 agggtggcgc tgaaaaagag gccggggccg gcgagcatcc aggggtaggt ctccatggag
     3541 tcggcgcctt cgcggatgag cacgccccag ctcgcgtcag gcggctggat gccgagtcca
     3601 aggaagctga gggtggcctc caggagcatg acgccgggga cggtgaggct ggcgtagatg
     3661 atgacggggc cgagcacgtt gggcagcatg tgcctgacca ggatgtgcgc gccgccggcg
     3721 ccgctggcgc gcgcggcggt gacgaactcg aggttcttca aagccagcac ctgcccgcgc
     3781 acgacgcgcg ccatggtcag ccactcgacg gcgccgatgg cggcaaagat gagccagagc
     3841 tcgcgcccga agacgacgac gagaatgatg acgaaggtga tgaaggggaa ggcgtagagg
     3901 atgtccacga ggcgcatcat gaaggcgtcc agccgccgcc cggccaggcc ggcgacgagg
     3961 ccgtagccga ctccgatgac ggaggcgacg agggtggcga cgatgccgac gagcagggag
     4021 acgcgcccgc cgtgcagggc gcgcgtggcg aggtcccgcc ccagggagtc ggtgcccagc
     4081 cagtgggcgg cggagggagc ggtggctttt aactccaggt cctggtcgtt cgggccgtag
     4141 ggtgagaaga gcggaccggc cacgcacacg gccaccagca ggatgaggaa ggcgagggcc
     4201 gccatggcga gccggttctg ccgcagcctg cgccaggcgg cctgccaggg gccggggt
//
LOCUS       DAOMYJ010000326         4211 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Prosthecobacter sp. isolate AMR_MDS_5629
            NODE_7446_length_4211_cov_25.247834, whole genome shotgun sequence.
ACCESSION   DAOMYJ010000326 DAOMYJ010000000
VERSION     DAOMYJ010000326.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867850
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prosthecobacter sp. (wastewater metagenome)
  ORGANISM  Prosthecobacter sp.
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Verrucomicrobiaceae; Prosthecobacter.
REFERENCE   1  (bases 1 to 4211)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4211)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.30
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:34:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,742
            CDSs (total)                      :: 4,684
            Genes (coding)                    :: 4,676
            CDSs (with protein)               :: 4,676
            Genes (RNA)                       :: 58
            tRNAs                             :: 55
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4211
                     /organism="Prosthecobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7446_length_4211_cov_25.247834"
                     /isolate="AMR_MDS_5629"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1965333"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1033)
                     /locus_tag="PLP58_21065"
     CDS             complement(<1..1033)
                     /locus_tag="PLP58_21065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ11085.1"
                     /translation="MKTTSLPLVSSLLVFLTAGCDPSAFQANSAAQDELRRQMEETRA
                     AYEQQAADMQARGESMQKELADLKLQMQEKENAELQARLLAMEEENRRLMADAETARQ
                     KADALRDQLLTQPQPQAPPVVVQPVAPVQPLPQAPVTQPWVDPAADYSLFYEQLSPHG
                     DWLDVEGYGYAWRPRLAAQASWRPYMDGRWMWSDHGWAWDSREPFGWACYHYGRWVRV
                     SRHGWVWIPGREWAPAWVSWRTGPDYIGWAPLPPGPGLNVTVISHDCDVRYGLSPSSY
                     FFIQSSYFGRSSYTSIGLSITNVTRIFASTVNVTSITVVNQSQSRFFAHRGGPDRAWL
                     ERRLGSPVPR"
     gene            1111..2031
                     /locus_tag="PLP58_21070"
     CDS             1111..2031
                     /locus_tag="PLP58_21070"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRJ11086.1"
                     /translation="MAVFLIRRLLSSLLVLFFAVSLTFLLTRSLPGGPFDREKLASAQ
                     VREALMAKYKLDGTLWQQYTTYMGDLLRGDLRVSFKYRDWTVAEILAQKLPVSLQLGA
                     LSFLLASTLGVLLGALAALRRDTPVDWLAMLGALLAISIPSFITGPVMIAVFALWLGW
                     LPVAGWGSPAHLVLPAICLAAPYTAYVARLMRNSLLDVLQSDFLRTAKAKGLSDAQAL
                     ARHAMKPAILPVVTFLGPLAAHLLTGSMIIESVFNISGAGGVFVNAIQNRDAFLLCGA
                     VVIYCSLLVFFNLVVDLLYRVLDKRIQLHG"
     gene            2138..3109
                     /locus_tag="PLP58_21075"
     CDS             2138..3109
                     /locus_tag="PLP58_21075"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF025010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HRJ11087.1"
                     /translation="MRPLILLAVLPVLSGAAAAQSMSNGIAAVVNGNPIMKSEVRDSV
                     NAQEQILRFQYQNDSNRLQKELSQLRATALDSLIDRELVLGEFKRLGASIKAQWVDDD
                     INSIVRESFKGDREAFIRELTSTGVTLKKFRELREKMMIVQAMRGKKAAEQPPATPKE
                     VEEYYRKNMDAWRSGGKVKISTITLSKLGTEPGFTLEGQRRIAEELRAKITKGADFAT
                     LAKTYSQDSKAEEGGSWDWMGREEMNPSIANVAFGMKAGQISEIIDDQAAYIIIACDA
                     IQHGPAKPMSEVREEIERAVSAEKSKAIIDQWMENLRKRAVIKKFGW"
     gene            complement(3193..>4211)
                     /locus_tag="PLP58_21080"
     CDS             complement(3193..>4211)
                     /locus_tag="PLP58_21080"
                     /inference="COORDINATES: protein motif:HMM:NF024578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HRJ11088.1"
                     /translation="EPPAERGQPGSRERPEQRGRQPETRRVPFIGVLTAPLSVDARAH
                     LKLAEGFGLRVVEVLPESPAKAAGLKEDDILIRFEDQRLASMEQLQALVRERRAGERV
                     ALTVISAGEQREVGVEIGENTVPARIEQGPPGPRFAPPPPGGRFPDSPPREGRPGPEG
                     PWRDWGHDLGRQMNEWREKLEQHQKAWREWQERVREWTQEHGRHFPPPPSMPGMPDFS
                     RREQPPEAQTGRIEDRREERREASTITRSDESGIYTLKREGDRVIFSATPRDGEQRSW
                     NLNDERERREIPEPLRDKLRQLEELRRDSQGAPPFRGEFREDEGNRQPPESRSSSSGP
                     RDRP"
BASE COUNT          723 a         1406 c         1352 g          730 t
ORIGIN      
        1 cgcgcggcac cgggctgcca aggcggcgct caagccaggc gcggtccggg ccgccgcgat
       61 gcgcgaagaa gcggctctgc gactggttga ccacggtgat gctggtcaca ttcactgtgg
      121 aggcaaaaat gcgggttacg ttggtgatgg acagcccgat gctggtgtag ctggagcggc
      181 caaaatagct gctctggatg aagaagtagc tgctggggct gagaccgtag cgcacatcac
      241 aatcgtggct gatgacggtg acattcaacc cggggccggg aggcagtggc gcccagccga
      301 tgtagtccgg ccccgtcctc caggagaccc atgcgggcgc ccattcgcgt ccgggaatcc
      361 acacccatcc gtgccgcgac acgcgcaccc agcgcccgta atgatagcag gcccaaccaa
      421 aaggctcgcg gctgtcccaa gcccatccgt ggtcgctcca catccagcgc ccgtccatgt
      481 agggccgcca ggaggcctgc gccgccagcc tgggacgcca ggcatagccg tagccttcga
      541 catccagcca gtctccatgg ggggagagct gctcataaaa gagcgagtag tcggcggcgg
      601 ggtccaccca gggctgggtc acgggcgcct ggggaagagg ctgcaccggc gccacgggct
      661 gaaccacaac gggcggggcc tgcggctgcg gctgggtcag cagttggtcg cgcagggcgt
      721 cggctttctg gcgcgcagtt tcagcatcgg ccatgaggcg gcggttttcc tcctccatgg
      781 ccagcagccg ggcctgcagc tcggcgttct ccttttcctg catttggagc ttcaagtcgg
      841 cgagttcttt ctgcatggac tcgccgcgcg cctgcatgtc ggcggcctgc tgctcataag
      901 cggcccgggt ttcctccatc tgccggcgca gctcgtcctg cgcggcggag tttgcctgga
      961 aggccgaggg gtcgcaaccc gcggtgagga acaccagcaa actggagaca agggggaggg
     1021 atgtcgtttt catggcaatg cctgaacaaa gacgaggcgc gggtgaatac cattcgattt
     1081 cacgcgcgga tgccgccatg atccgcgcgc atggccgtct ttctcatccg tcgtttgctc
     1141 agcagcctgc tggtgctgtt cttcgcggtg tcactcactt tcctgctgac gcgctcgctg
     1201 ccgggcgggc cttttgacag ggaaaagctg gcctccgcgc aagtgcggga ggcgctgatg
     1261 gccaagtaca agctggatgg cacgctgtgg cagcagtaca cgacttacat gggcgacctg
     1321 ctgcgcggcg acctgcgtgt gtccttcaaa taccgcgact ggacggtggc ggagatcctg
     1381 gcgcaaaagc tgcccgtctc actgcagctc ggcgcgctgt ccttcctgct cgcaagcacg
     1441 ctcggcgtgc tgctcggcgc gctggcggcg ctgcggcggg acacgccggt ggactggctg
     1501 gccatgctcg gcgcgctgct cgccatctcg atcccatcct tcatcaccgg gccggtgatg
     1561 atcgccgtct tcgcgctctg gctgggctgg ctgccggtgg cgggctgggg cagcccggcg
     1621 catctggtgc tgcccgccat ttgcctggcc gccccctaca cagcctatgt ggcgcggctg
     1681 atgcgcaaca gcctgctgga tgtgctccag agcgacttcc tgcgcacggc caaggccaag
     1741 ggcctctccg acgcgcaagc gctggcgcgc catgccatga agcccgccat cctgcctgtg
     1801 gtgacttttc tcggcccgct ggcggcgcat ctgctcacgg gttcgatgat catcgagagc
     1861 gtcttcaaca tctccggcgc gggcggagtc ttcgtcaacg ccattcagaa ccgggacgcc
     1921 tttctgctct gcggagcggt ggtcatctac tgctccctgc tggtcttctt caacctcgtg
     1981 gtggacctgc tttaccgagt gctcgacaag cgcatccagc tccacggctg acggacaccg
     2041 tgccgccagg gccaaaatgc gcgccggttt tggccttgcc ggagcgttca atggtgagta
     2101 agcgtcacgt ccacccccgc gtcctcgatc catgtccatg cgtcccctca tccttctggc
     2161 cgttctgcca gtcctatccg gcgccgccgc agcccagtcc atgagcaacg gcatcgccgc
     2221 cgtggtcaac ggcaacccca tcatgaagtc cgaggtgcgc gactcggtga acgcccagga
     2281 gcagatcctc cgctttcaat accagaacga ctcgaaccgg ctgcagaagg agctttccca
     2341 gctccgcgcc accgcgctgg acagcctcat agaccgggag ctggtgctcg gagaattcaa
     2401 gcgcctgggc gccagcatca aagcccagtg ggtggatgac gacatcaaca gcatcgtgcg
     2461 cgagagcttc aagggcgacc gcgaggcctt catccgggag ctgaccagca ccggagtcac
     2521 cttgaagaaa ttccgcgaac tgcgcgaaaa gatgatgatc gtgcaagcca tgcgcgggaa
     2581 aaaagccgcc gagcagcctc cggccacgcc aaaggaagtg gaggagtatt accggaagaa
     2641 catggatgcc tggcgcagcg gcgggaaggt gaagatcagc accatcacgc tctccaaatt
     2701 aggcacggaa cccggcttca ccctcgaagg ccagcgcagg atcgccgagg agctgcgcgc
     2761 caaaatcacc aaaggcgcgg actttgccac cctggcgaag acttactccc aggacagcaa
     2821 ggccgaggaa ggcggctcat gggactggat ggggcgcgag gaaatgaacc cctccatcgc
     2881 caacgtggcc tttggcatga aggcgggaca gatcagcgaa atcattgacg accaggcggc
     2941 ctacatcatc atcgcctgcg acgccatcca gcatggcccc gccaagccga tgagcgaggt
     3001 gcgcgaggag atcgagcgag ccgtctccgc cgaaaagtcc aaggccatca ttgaccagtg
     3061 gatggaaaac ctgcgcaagc gcgcggtcat caaaaaattt ggctggtaag ccccgtgcca
     3121 aaaggaaagc agggacgcgc ggctcccgac acacgcgccc ctgctccttg gttgcggggg
     3181 ttggatgcaa aatcaaggac ggtcacgagg accgctggaa gaagaacgac tctcaggcgg
     3241 ctggcggttg ccttcatctt cacgaaattc acccctgaaa ggcggcgcgc cctgcgagtc
     3301 acgccgcaat tcctctagct ggcgcagctt gtcgcgcagc ggttcgggga tttcccggcg
     3361 ctcgcgctcg tcgttcagat tccaactgcg ctgctccccg tccctgggcg tggcggagaa
     3421 aatcacccgg tcgccctcgc gcttgagcgt gtagatgccg ctctcgtcgc tgcgggtgat
     3481 ggtggaggcc tcccgcctct cctcgcggcg gtcctcaata cgcccggtct gcgcttcggg
     3541 cggctgctcg cgccgggaaa aatccggcat gccaggcatc gaaggcggcg gcgggaaatg
     3601 cctgccgtgc tcctgggtcc actcgcgcac gcgctcctgc cattcacgcc aggccttctg
     3661 atgctgctcc agcttctccc gccattcatt catctgccgt ccaaggtcat ggccccagtc
     3721 gcgccaggga ccctccgggc cgggtctgcc ttcgcgcggc ggcgagtccg ggaacctgcc
     3781 tcccggaggc ggaggtgcga agcgcggtcc tggaggcccc tgctcgatcc gtgctggcac
     3841 ggtgttttca ccgatctcca cgcccacctc gcgctgctcg cccgcggaga tgaccgtgag
     3901 cgccacgcgc tctcccgcgc gccgttcccg caccagggcc tggagctgct ccatgctggc
     3961 gaggcgctgg tcttcgaagc ggatcaaaat gtcgtcctcc ttcagtcccg ccgctttggc
     4021 gggactctct ggcagcacct cgacgacgcg caggccgaag ccctcggcca gcttgagatg
     4081 cgcgcgcgca tccacgctca aaggcgcggt gagcacgccg atgaaaggca ccctgcgggt
     4141 ttccggctgg cggccgcgct gctccgggcg ctcgcgactc cccggctgcc cgcgctccgc
     4201 tggcggctcc g
//
LOCUS       DAOMYJ010000327         4180 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Prosthecobacter sp. isolate AMR_MDS_5629
            NODE_7543_length_4180_cov_20.640242, whole genome shotgun sequence.
ACCESSION   DAOMYJ010000327 DAOMYJ010000000
VERSION     DAOMYJ010000327.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867850
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prosthecobacter sp. (wastewater metagenome)
  ORGANISM  Prosthecobacter sp.
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Verrucomicrobiaceae; Prosthecobacter.
REFERENCE   1  (bases 1 to 4180)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4180)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.30
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:34:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,742
            CDSs (total)                      :: 4,684
            Genes (coding)                    :: 4,676
            CDSs (with protein)               :: 4,676
            Genes (RNA)                       :: 58
            tRNAs                             :: 55
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4180
                     /organism="Prosthecobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7543_length_4180_cov_20.640242"
                     /isolate="AMR_MDS_5629"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1965333"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2200)
                     /locus_tag="PLP58_21085"
     CDS             complement(<1..2200)
                     /locus_tag="PLP58_21085"
                     /inference="COORDINATES: protein motif:HMM:NF019219.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1549 domain-containing protein"
                     /protein_id="HRJ11089.1"
                     /translation="MALPRSILLLCLASAALSPAPAEVDYARDIQPILAEHCLLCHGP
                     DDSKGGLTLTSFELATNALKSGAHAIMPGKPEQSAVIERMTTTDPDEQMPPPEQRAKK
                     PVTARETELLRQWIAAGAKYDLHWAYKPVVRPAVPEVRNPQSKIRNAIDAFIQQKLAA
                     QGIAPSPEADAHTLVRRLSLDLTGLPPSLDLIERHCQPGQSSLTDAAYERLVDELLQS
                     PHFGERWGRHWLDMARYADSDGYEKDRPRPDAWRFRDWVIRAINDDMPFDQFTIEQLA
                     GDLLPDATAEQLVATAFNRQTLTNTEGGTDQEQFRVEACMDRAETVGTVWLGLTVGCA
                     RCHTHKYDQITIQEYYQLYAFFDNGDEATRQAPVSPEAWAEYEKKNGQAARLLIPLQK
                     RLDEAKAALPLKLPEWEKAMQARLAKARADKPALQHEPVKIAKVDSQAGAKFQTLEDG
                     SVLASGKQIKDERYTVRLAPSDKAVTSLQIEVLPHDTLPKQGPGRSSGGNFVLSEVRV
                     TAAGKTLVAHSPKASFEQKTFGATGAVDGKGDTGWAVSGGAGKAHKLTLHLAEPVPAG
                     AEITLELDQNYAKDATHTLGRFRILAASQETEETLLPAAVIRILNEEPRRRNPVVIQA
                     LYDWLEKTDADVIAAAAALQNAQDKLPKPPLMDVRVIAQRAQNPRQSHVLHRGDFLQP
                     AEPVTPAVLGVASQPGAKREASTRLDLARWLVSRENPLTARVTVNHIWARL"
     gene            complement(2248..3888)
                     /locus_tag="PLP58_21090"
     CDS             complement(2248..3888)
                     /locus_tag="PLP58_21090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009958674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase"
                     /protein_id="HRJ11090.1"
                     /translation="MKTALALAFTLLFCLNLRAADRPNILFLFADDWGRYASIYAEAN
                     GAGGINDVVRTPNFDRIAKKGVLFRNAHVNAPSCTPCRSSLLSGQYFWRTGRGAILRG
                     AVWDDKIPAYPLLLKDSGYHIGKTYKVWSPGTPADAPYGQQAHSYQKAGGKFNQFSQN
                     VTKMVAEGMDLQAAKEVLYDEVRGNFRDFLAAREAGKPFCYWFGPTNVHRTWIKGSGK
                     ALWDINPGDLKGKMPAFLPDVPEVREDLADYFGEIAAWDHAIGLLIEAVEKAGELDNT
                     LIVISGDHGAPGFPHGKCNLYGFGTGVSLSITGPGVQGGRVVDDFVNLTDLAPTFLEA
                     GGVAVPDVMTGRSLWPVLKSGKTGLVDPRRTWVVTGRERHVEVARADYTPYPQRALTT
                     HDHLFIINFRPDRWPLGDPYGLRPGETEPTAEEITQTTRVTHPDEDAGPTKAWLIGAR
                     NSAEWAGHYQWVYGQRPKYELYDLKKDPHETTNLADDPAYAAVKAEMEKRLLDELRRT
                     GDPRLIDDGRFFETPPMAGPLEDEKEFWNKGKGRGKKK"
     gene            complement(3949..>4180)
                     /locus_tag="PLP58_21095"
     CDS             complement(3949..>4180)
                     /locus_tag="PLP58_21095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009964308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF1501 domain-containing protein"
                     /protein_id="HRJ11091.1"
                     /translation="GGAIGGVMGGVKAGHAHGATDDIGFQAVDGKVHMHDLHATILHL
                     LGLDHEKLTFRYAGRDFRLTDVHGHVVKEILA"
BASE COUNT          595 a         1190 c         1566 g          829 t
ORIGIN      
        1 acaggcgcgc ccagatgtgg ttgacggtga cgcgcgcggt gaggggattt tcccggctga
       61 cgagccagcg ggcgaggtcg aggcgagtgg aggcttcacg cttggctccg ggctggctgg
      121 ccacgccgag aacggcgggc gtgacgggtt cggcgggctg gaggaaatca ccacggtgca
      181 gcacatggga ctggcgcgga ttttgagcgc gctgggcgat gacacgcacg tccatgagcg
      241 ggggcttggg cagcttgtcc tgcgcgtttt gcagggcggc ggctgcggcg atgacatcgg
      301 cgtcggtttt ttccagccag tcatagagcg cctggatgac cacgggattc cggcggcgcg
      361 gctcctcgtt gagaatgcgg atgacagcgg cagggagcag agtttcctcg gtctcctggc
      421 tggcggcgag gatgcggaag cggccgaggg tgtgcgtggc gtccttggcg tagttttgat
      481 cgagttcgag cgtgatctcc gcgcccgcgg gaacgggttc ggcgagatgg agcgtgagct
      541 tgtgcgcctt gcccgcgccg ccgctgacgg cccagccggt gtcgcctttg ccatccactg
      601 cacctgtcgc gccgaaggtt ttttgctcaa agctggcctt cggcgaatgc gccacgaggg
      661 tcttgccggc ggctgtcacg cgcacttcgc tgagcacgaa gttgccgccg ctgctgcgtc
      721 cggggccttg cttgggcagt gtgtcgtggg gcaagacctc gatctgcaag gaagtgaccg
      781 ctttgtccga aggggccagc ctcaccgtgt agcgctcgtc tttgatctgc ttgccgctgg
      841 cgagcacgga gccgtcttcg agagtttgaa atttcgcgcc ggcttggctg tccaccttgg
      901 cgattttcac cggctcgtgc tggagcgcgg gtttgtccgc gcgcgctttc gccagccttg
      961 cctgcatggc tttttcccac tcgggcagct tcaaaggcag ggcggctttg gcttcgtcga
     1021 gccgcttttg cagcgggatg agcaggcgcg cggcctggcc gtttttcttt tcatactcgg
     1081 cccatgcctc gggggaaacg ggcgcctggc gggtggcttc gtcgccattg tcgaagaagg
     1141 cgtagagctg atagtactcc tggatggtga tctggtcata cttgtgggta tggcagcgcg
     1201 cgcagccgac ggtgaggccg agccagacgg tgccgacggt ctcggcgcgg tccatgcagg
     1261 cctccactcg gaactgctcc tggtcggtgc cgccctcggt gttggtgagg gtctggcggt
     1321 tgaaggcggt ggccacgagc tgctcggcgg tggcgtcggg caggaggtcg ccggcgagct
     1381 gctcgatggt gaactggtca aagggcatgt cgtcattgat ggcgcggatg acccagtcgc
     1441 ggaagcgcca ggcatccggg cgcgggcggt ccttctcata gccgtcgctg tcggcgtagc
     1501 gcgccatgtc gagccagtgc cggccccagc gctcaccgaa gtgcgggctc tgcaaaagct
     1561 cgtccacgag ccgctcgtag gcggcgtcgg tgagacttga ctgaccgggt tgacagtggc
     1621 gctcgatcag gtcgagcgag ggcggcaggc ctgtgaggtc cagggaaaga cggcgcacga
     1681 gggtgtgcgc gtcggcctcc ggtgaggggg cgatgccttg agcggcgagc ttttgctgga
     1741 tgaaggcgtc tatggcattg cggattttcg attgcgggtt tcgtacttcc ggcaccgccg
     1801 gcctaaccac aggcttgtaa gcccaatgca ggtcgtattt ggcccctgcg gcgatccatt
     1861 gccggagcag ttcggtctcg cgcgcggtaa cgggtttttt ggcgcgctgc tcgggcggcg
     1921 gcatttgctc gtcgggatcg gtggtggtca tgcgctcgat gacggcgctc tgctccggct
     1981 tgccgggcat gatggcgtgc gcgccgcttt tgagggcatt ggtggcgagt tcgaaggagg
     2041 tgagtgtaag gccgcccttt gagtcgtcag ggccgtggca gagcaggcag tgctccgcca
     2101 ggatgggctg gatgtcgcgc gcgtaatcca cctccgccgg ggcgggggag agcgcggcgc
     2161 tggcgaggca gagcaggagg atggaccggg ggagtgccat gcccatttaa cgtcggcgcg
     2221 gggcgggctt tgtcggcgtg aggacgatca cttcttcttc cccctgcctt tgcccttgtt
     2281 ccaaaactcc ttctcatcct ccagcgggcc ggccatgggc ggagtctcga agaacctgcc
     2341 gtcgtcaatg aggcgcgggt cgccggtgcg gcggagttcg tcgagcaggc gcttttccat
     2401 ctcggctttg acggcggcgt aggcggggtc gtcggcgaga ttggtggtct cgtgcggatc
     2461 ttttttgagg tcgtagagtt cgtatttggg gcgctgcccg tagacccatt ggtaatgccc
     2521 cgcccattcc gccgagttgc gcgcgccgat gagccaggcc ttcgtcgggc cggcgtcctc
     2581 gtcggggtgg gtgacgcgcg tggtttgtgt gatctcctcg gcggtgggct cggtctcgcc
     2641 ggggcgcagg ccgtaggggt cgccaagcgg ccagcggtcg gggcggaagt tgatgatgaa
     2701 aagatgatcg tgcgtggtga gcgcgcgctg cgggtagggc gtgtagtcgg cgcgggcgac
     2761 ttcgacatgg cgctcgcggc cggtgacgac ccaggtgcgt cgcgggtcca cgaggccggt
     2821 tttgccggat ttcaaaacgg gccagaggct gcgcccggtc atgacatccg gcacggccac
     2881 gccgccggct tcgaggaagg tgggggcgag gtcggtgagg ttgacgaagt catccacgac
     2941 acggccgcct tggactcccg ggccggtgat ggagagggag acgccggtgc cgaagccgta
     3001 gaggttgcac ttgccatgcg ggaagccggg ggcgccgtgg tcgccgctga tgacgatgag
     3061 ggtattgtcg agttccccgg ctttttccac cgcctcgatg agcaaaccga tggcgtggtc
     3121 ccaggcggcg atctcgccga agtagtcggc gaggtcctcg cgcacctcgg gcacgtcggg
     3181 caggaaggcg ggcattttgc ctttgaggtc gccggggttg atgtcccaga gggctttgcc
     3241 gctgcctttg atccaggtgc ggtggacgtt ggtggggccg aaccagtagc agaagggttt
     3301 gccggcctcg cgcgcggcga ggaagtcgcg gaaattcccg cgcacctcgt cgtagagcac
     3361 ttctttggcg gcctggaggt ccatgccttc ggcgaccatc ttcgtcacgt tctgggagaa
     3421 ctggttgaac ttgccgcccg ccttttgata cgagtgcgcc tgctggccgt aaggcgcgtc
     3481 cgccggtgtg ccgggactcc acactttata ggtcttgccg atgtggtagc cggagtcttt
     3541 gagcaggagc gggtaggcgg gaattttgtc atcccacacc gcgccgcgca ggatggcgcc
     3601 gcgtccggtg cgccagaagt actggccgga gagcagggaa ctgcggcagg gcgtgcagga
     3661 gggggcgttg acgtgggcgt tgcggaagag cacgcctttt ttcgcgatcc tgtcgaagtt
     3721 cggcgtgcgc accacgtcgt tgatgccgcc tgccccattc gcctcggcat agatgctggc
     3781 gtagcgcccc cagtcgtcgg cgaaaaggaa gaggatgttc ggccggtccg cggcgcgcag
     3841 gttcaggcaa aaaagaaggg tgaatgcaag ggcgagggcg gtcttcatgg tcggcgcagg
     3901 agaacgcgcc gcgcatggcg ggctttcggg aatgtcgccc ggcccggttc aagccaggat
     3961 ctctttcacc acatgtccat gcacatcggt gaggcggaag tcgcgcccgg cgtaacggaa
     4021 ggtgagcttt tcgtggtcga gaccgagcag gtgcaggatg gtggcgtgca ggtcgtgcat
     4081 gtggactttg ccatccacgg cctggaagcc gatgtcgtcc gtggcgccgt gcgcgtgccc
     4141 ggccttcacg ccgcccatca cgccaccgat cgcgccacca
//
LOCUS       DAOMYJ010000328         4158 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Prosthecobacter sp. isolate AMR_MDS_5629
            NODE_7593_length_4158_cov_18.961979, whole genome shotgun sequence.
ACCESSION   DAOMYJ010000328 DAOMYJ010000000
VERSION     DAOMYJ010000328.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867850
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prosthecobacter sp. (wastewater metagenome)
  ORGANISM  Prosthecobacter sp.
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Verrucomicrobiaceae; Prosthecobacter.
REFERENCE   1  (bases 1 to 4158)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4158)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.30
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:34:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,742
            CDSs (total)                      :: 4,684
            Genes (coding)                    :: 4,676
            CDSs (with protein)               :: 4,676
            Genes (RNA)                       :: 58
            tRNAs                             :: 55
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4158
                     /organism="Prosthecobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7593_length_4158_cov_18.961979"
                     /isolate="AMR_MDS_5629"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1965333"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            228..1634
                     /locus_tag="PLP58_21100"
     CDS             228..1634
                     /locus_tag="PLP58_21100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009958246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HRJ11092.1"
                     /translation="MPNPANLHSMGKEFDTTPQNVPHVSTRHRTIRTPVPHPDSLPHI
                     ERSRKYEPLSMRGMPPIVWERAEDIYVFDKHGNRWLDWSSGVLVTNCGHGVPEVRQAI
                     IDQVNSGLIHNYVFPSEERPELAELIASVSPEPLKKVFLLSTGSEATECAIKLSRAHG
                     IAKGGKEKIGIIGFDRGYHGRTLASQQAGGMAGQKTWIVNLDPAIINVPFPDGYWEAD
                     SSFEGFLQAVEKSGMRPAQIAGVIMESYQGVGPDFAPVEYIQKLRAWCDEHDVVLTFD
                     EVQSGFGRTGKFWAFEHYGVVPDLICCGKGISSSLPLSAVIGRQDIMDQFPRGSMTST
                     HTGNPVCCAAALANIRKILREDLTGNAARLDPILLAGCKAIQARHPEVIGNVTCAGLV
                     AGLQTVKRGTKQPCHDLAHRVIELCYQKGLLFFAPVGAWGQTVKICPPLTIPQDALEE
                     GLAVLGEAFDEAVAERSA"
     gene            1765..3651
                     /gene="nagB"
                     /locus_tag="PLP58_21105"
     CDS             1765..3651
                     /gene="nagB"
                     /locus_tag="PLP58_21105"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009963436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004342 - glucosamine-6-phosphate
                     deaminase activity [Evidence IEA];
                     GO_process: GO:0006046 - N-acetylglucosamine catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HRJ11093.1"
                     /translation="MSRRPPTEAYEKTPTHIFPDSAAAAKALAAEVRALIEERAAQGK
                     HAVLGMATGSTPVPFYRELIRLHKEEGLSFKNVITFNLDEYYGLGPDHPESYACFMRE
                     QLFDHVDIPKASINIPSGTVPGDQVFAHCREYEEKIDAVGGIDLQILGIGRTGHIGFN
                     EPGSSRDSLTRRITLDRVTRQDAAADFRGEENVPHFAITMGVGTILRGKKLVLMAWGE
                     NKAGMVAKALEGPVTEAISASFLQGHPDARFFIDTGASRELTRIKLPWLVGPVSWTPR
                     ETRRAVCWQVFKTGRPVLKLTDEHYNEHGLSDLLSEQGPAYQLNIRIFNQLQHTITGW
                     PGGKPSEDDTYRPERATPYPKRVLILSPEPQDAIVAMGSTLERLVEQGHEVRLTALTS
                     GNLRVPDSEADKFAGTLQELAGTVENPAAWAEQTAYAREALRLLEEKGEFGEDPPLIR
                     QLKALILRGELRDAAHACNLGTDRVTFLDLPFYEQGRYRRFRGTQTDVDALAALLRRE
                     KPHQIYTTGDAADPSSVSGICHRLLQAALKSCAGEEWAGTCSLWLYRGKEKALEPHEI
                     DMAVPLSPMQLERKARALSRYGSLSTLELSAPETNRENARHYDSLGLAEYEAIECFQR
                     WVRA"
     gene            complement(3744..>4158)
                     /locus_tag="PLP58_21110"
     CDS             complement(3744..>4158)
                     /locus_tag="PLP58_21110"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HRJ11094.1"
                     /translation="TPRPGVPGEGHVLIVDDEAPLRAFARQCLVNNGYQVSETDNGQD
                     AVRLYQSLRRSGRTPDVVLMDLTLRGGMNGVEAAGEILRFDPDARIVVCSGSVNQDVQ
                     NIFLEKGFLAALPKPYDVAELTRTVSDVITMMSRH"
BASE COUNT          742 a         1578 c         1219 g          619 t
ORIGIN      
        1 acctcggcgg cgcgggcgag cttttcctgc cccaggcccg cgccggccag gagcagcagg
       61 cacggcgtga tgatccagac tttcatgacc cgcgcatttg accgcctccc cccgccggat
      121 gcaacgcccg ctttcccaag acacgcaagc ggcctggaca ttcagcacaa aaataccgtg
      181 tgccgtgaga tgggcagtag cgcgccacgc ccgccgcggg cagcatcgtg ccgaaccccg
      241 ccaacctgca ttccatgggc aaagaattcg acaccacccc gcaaaacgtc ccgcacgtct
      301 cgaccaggca ccgcaccatt cgcacccccg tgccgcaccc ggactcgctc ccgcacatcg
      361 agcgcagccg caagtacgag ccgctgtcca tgcgcggcat gccgcccatc gtctgggagc
      421 gcgcggagga catttatgtc ttcgacaaac acggcaaccg ctggctggac tggagcagcg
      481 gcgtgctcgt caccaactgc ggccacggcg tgcccgaggt gcgccaggcc atcattgacc
      541 aggtcaacag cgggctgatc cacaactacg tcttccccag tgaggagcgc cccgagcttg
      601 cggagctgat cgccagcgtc tcgcccgagc ctctgaaaaa agtcttcctc cttagcaccg
      661 gcagcgaggc caccgagtgc gccatcaaac tcagccgcgc ccacggcatc gccaagggcg
      721 gcaaggagaa gatcggcatc atcggcttcg accgcggcta ccacggccgc accctcgcca
      781 gccagcaggc cggcggcatg gccgggcaga agacctggat cgtcaatctc gaccccgcca
      841 tcatcaacgt ccccttcccc gacggctact gggaggctga cagcagcttc gagggctttt
      901 tgcaggccgt ggagaaaagc ggcatgaggc ccgcgcagat cgccggcgtc atcatggaga
      961 gctaccaggg cgtcggcccc gacttcgccc ccgtcgagta catccagaag ctgcgcgcct
     1021 ggtgtgacga gcacgacgtg gtgctcacct ttgacgaggt gcagtccggc tttggccgca
     1081 cgggcaagtt ctgggccttc gagcactacg gcgtggtgcc cgacctcatc tgctgcggca
     1141 agggcatctc ctcctccctg cccctgagcg ccgtcatcgg caggcaggac atcatggacc
     1201 agttcccccg cggctccatg accagcaccc acaccggcaa ccccgtctgc tgcgccgccg
     1261 cgctggccaa catccgcaaa atcctgcgcg aggacctcac cggcaacgcc gcccgccttg
     1321 atcccatcct gctcgcaggc tgcaaggcca tccaggcacg ccaccccgaa gtcattggca
     1381 acgtcacctg cgcgggcctc gtcgccggat tgcagaccgt caagcgcggc accaagcagc
     1441 cctgccacga cctggcgcac cgcgtcatcg aactgtgcta ccagaagggg ctgctcttct
     1501 tcgcgccggt gggcgcctgg ggccagaccg tgaaaatctg cccgccgctc accatcccgc
     1561 aggacgcgct ggaggagggc ctcgccgtgc tgggtgaggc gtttgacgaa gctgtggcgg
     1621 aacggtcggc gtgacaatca cggtggtgat ctttctgctg gcgaatctct ccgcctgagg
     1681 ggacgcgccg ccgagcaagt tgccactgtc tcagcccttg acggaaccct gtcctgcggt
     1741 ccgttgtttt ccaaaccaac caccatgtca cgccgcccgc ccaccgaagc ctacgagaag
     1801 acacccaccc acattttccc cgactccgcc gccgccgcaa aggcgctcgc cgccgaggtg
     1861 cgcgcgctca ttgaggagcg cgccgcgcag gggaagcacg ccgtgctcgg catggccacc
     1921 ggctccacgc ccgtgccctt ttatcgcgag ctcatccgac tgcacaagga ggagggcctc
     1981 agcttcaaaa acgtcatcac cttcaatctc gacgaatatt acggcctcgg tcccgaccac
     2041 cccgagagct acgcctgctt catgcgcgag cagctctttg accatgtgga catccccaag
     2101 gcgagcatca acatccccag cggcaccgtg ccgggggatc aggtcttcgc ccactgccgc
     2161 gagtatgagg agaaaataga cgccgtcggc ggcatagatc tgcaaatcct cggcatcggc
     2221 cgcaccggcc acatcggctt caacgagccc ggctccagcc gcgactccct gacccgccgc
     2281 atcacgctcg accgcgtcac ccgccaggac gccgccgccg acttccgcgg tgaggaaaac
     2341 gtgccccact tcgccatcac catgggcgtc ggcaccatcc tgcgcggcaa aaaactcgtc
     2401 ctcatggcct ggggcgaaaa caaagccggc atggtcgcca aagccctcga aggccccgtc
     2461 accgaggcca tctccgcctc cttcctccag ggccaccccg acgcccgctt cttcatagac
     2521 accggcgcct cacgcgagct cacccgcatc aagctcccct ggctcgtcgg ccccgtctcc
     2581 tggaccccgc gcgagacgcg ccgcgccgtc tgctggcagg tcttcaaaac cggccgcccc
     2641 gtcctcaagc tcaccgacga gcactacaac gagcacggcc tcagcgacct cctctccgag
     2701 caaggccccg cctaccagct caacatccgc atcttcaacc agctccagca caccatcacc
     2761 ggctggcccg gcggcaagcc cagcgaggac gacacctacc gccccgagcg cgccaccccg
     2821 tatccgaagc gcgtcctcat cctcagcccc gagccgcagg acgccatcgt cgccatgggc
     2881 tccaccctcg agcgcctcgt cgagcagggg cacgaggtcc gcctcaccgc cctcaccagt
     2941 ggcaacctgc gcgtgcccga cagcgaggcc gacaaattcg ccggcaccct ccaggagctg
     3001 gccggcaccg tcgaaaaccc cgccgcctgg gccgagcaga ccgcctacgc ccgcgaggcc
     3061 ctccgcctcc tggaggaaaa aggcgagttc ggcgaggacc cgcccctcat ccgccagctc
     3121 aaagccctca tcctgcgcgg cgagctgcgt gacgccgccc acgcctgcaa cctcggcacc
     3181 gaccgcgtca ccttcctcga cctccccttc tacgagcagg gccgctaccg ccgcttccgc
     3241 ggcacccaga ccgacgtgga cgccctcgcc gccctgctgc gccgggaaaa gccgcaccag
     3301 atttacacca ccggcgacgc cgccgacccc agcagcgtct ccggcatctg ccaccgcctc
     3361 ctccaagccg ccctgaaaag ctgcgccggc gaggaatggg ccggcacctg ctccctgtgg
     3421 ctctaccggg ggaaggaaaa agccctcgag ccgcacgaga tagacatggc cgtgccgctc
     3481 agccccatgc agctcgagcg caaagcccgc gccctctccc gctacggcag cctctccacc
     3541 ctcgagctct ccgcccccga gaccaaccgc gaaaacgccc gccactacga ctccctcggc
     3601 ctcgccgaat acgaggccat cgagtgcttc cagcgctggg tgcgggcgtg agtctggcag
     3661 attgtttttc gatccatcaa gtgacgcaca gaggctggct catgggctga gtgctgcccc
     3721 tccctggccg ccggcatgtt ttttcaatgc ctgctcatca tggtgatgac atcgctgacg
     3781 gtgcgtgtga gttcggcgac gtcgtagggc ttgggcaggg cggcgaggaa gcctttttcc
     3841 aggaagatgt tttgcacgtc ctggttgacg ctgccgctgc agacgacgat gcgggcgtcg
     3901 gggtcgaagc ggaggatctc gccggcggcc tcgacgccgt tcatgccgcc gcgcagggtg
     3961 aggtccatga gcacgacgtc gggggtgcgc ccggagcggc ggaggctttg gtagaggcgg
     4021 acggcgtcct ggccgttgtc ggtctcgctg acttggtagc cgttgttgac gaggcattgg
     4081 cgggcgaagg cgcggagggg ggcctcgtcg tccacgatga gcacatgccc ctcgccgggg
     4141 acgccgggcc tgggggtg
//
LOCUS       DAOMYJ010000329         4149 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Prosthecobacter sp. isolate AMR_MDS_5629
            NODE_7618_length_4149_cov_22.550073, whole genome shotgun sequence.
ACCESSION   DAOMYJ010000329 DAOMYJ010000000
VERSION     DAOMYJ010000329.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867850
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prosthecobacter sp. (wastewater metagenome)
  ORGANISM  Prosthecobacter sp.
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Verrucomicrobiaceae; Prosthecobacter.
REFERENCE   1  (bases 1 to 4149)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4149)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.30
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:34:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,742
            CDSs (total)                      :: 4,684
            Genes (coding)                    :: 4,676
            CDSs (with protein)               :: 4,676
            Genes (RNA)                       :: 58
            tRNAs                             :: 55
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4149
                     /organism="Prosthecobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7618_length_4149_cov_22.550073"
                     /isolate="AMR_MDS_5629"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1965333"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..750
                     /locus_tag="PLP58_21115"
     CDS             <1..750
                     /locus_tag="PLP58_21115"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HRJ11095.1"
                     /translation="GWLWGRLPLRLRAALPHGAEPFWLLPPMLLLAWAGWELGPVLER
                     HWMVAVLPDGSKAADFRLWWPQFTGLPFDQRNGMVVGFMMGFAVIPVIFTIAEDALSN
                     VPGELVAASEALGASRWQVLRTVVLPVASAGIFSALMIGLGRAVGETMIVVMATGNTP
                     VMDETGRLLLGDGLLGGGKFPLAAHWNPFDGMRTLSANLAVELPEAPQGSTHYRTLFL
                     GAFVLFVLTFALNTAAELLRQRLRRKFKVGR"
     gene            complement(821..1090)
                     /locus_tag="PLP58_21120"
     CDS             complement(821..1090)
                     /locus_tag="PLP58_21120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ11096.1"
                     /translation="MHAAALAVLLFLLARHEVCFSLLKTTLVVFGVSLICVLLELWRP
                     LAPLAGFAVITPLALKRFFHMRTRIVVAVTIPFMCWLTLTYVPFW"
     gene            complement(1165..1506)
                     /locus_tag="PLP58_21125"
     CDS             complement(1165..1506)
                     /locus_tag="PLP58_21125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ11097.1"
                     /translation="MFRQAPLAALAVMLFTFTDGVVSLHELLFVIPVSALFLTGLTLG
                     WRTGQPFWNILTWVALMAGSRMAGVHDAGVSLALFLQWLCIVIILTGLPLLMFPAWFR
                     EKGRILPDKWE"
     gene            complement(1530..1829)
                     /locus_tag="PLP58_21130"
     CDS             complement(1530..1829)
                     /locus_tag="PLP58_21130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ11098.1"
                     /translation="MLGLIVNTVALMAILWMLARHEADLSFGRTMLVVFGITFGCGLL
                     GLLHPLAPLAAFAVVTPLALKFFFYLRTGPAFGATGLFLVWLVVWELLWAWLRRP"
     gene            2136..2528
                     /locus_tag="PLP58_21135"
     CDS             2136..2528
                     /locus_tag="PLP58_21135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ11099.1"
                     /translation="MPDPSVLIVHAFFFGILVFLLARTALAERKSRRLLFACTVFAWT
                     CLIYQLAGWARSLGYNLHYSLATSVLLNSCAGALEEGQHEDVTRELRQLTDEFRVTYE
                     NRGDFMELALKAVNSLNGTQAQEQKQKE"
     gene            complement(2709..3962)
                     /locus_tag="PLP58_21140"
     CDS             complement(2709..3962)
                     /locus_tag="PLP58_21140"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HRJ11100.1"
                     /translation="MPSRFTFLQYTLGSFQEAAARVKGAGTKLTDHLGKAAYSFRLLR
                     YWWAGCAIAAEARRAGRPLVVVDAGCERGWLRHFTPVGAVERWIGLDWNPREEVRQLA
                     CYDEVRHANFDDPLPLASGIADVVVSLHVFEHLPRPGATLAELSRVLKPGGIFLGCTP
                     TMPGWLARLREGYLRASLRRGKVVPGGHITVLSPGRWHSLARDAGLEPEFVTGSHAVR
                     RTGGWLENFPAWVRLNQLWGALFPALGSECCLQARRQDPWDYKAAKLESRGRHLRPVW
                     ISLTAAAALALGWAWLAARDAHQIHQRAAIMSWLESHQKGGDVFILADCVSHLGDATR
                     EDVFEARDREELESILRKHPHSHILVSEHTARRFVSQYPPLTWRVDSWFEIGEEDFLM
                     LRTWPEGTPLDEYLLGMASGANRAE"
BASE COUNT          809 a         1320 c         1297 g          723 t
ORIGIN      
        1 ggctggctct gggggcggct gcccctgcgc ctgcgcgccg ccctgcctca tggcgcggag
       61 cccttctggc tgctgccgcc catgctgctg ctggcctggg ccggctggga gttgggaccg
      121 gtgctggagc ggcactggat ggtggccgtg ctgccggacg ggtcaaaggc ggccgacttc
      181 cgcctctggt ggccgcagtt caccggcctg ccctttgacc agcgcaatgg catggtcgtc
      241 ggcttcatga tgggcttcgc cgtcattccc gtcatcttca ccatcgccga ggacgccctc
      301 tcgaacgtgc ccggcgagct cgtggccgcc tccgaggcgc tcggcgccag ccgctggcag
      361 gtgctgcgca ccgtcgtcct gcccgtggcc tccgccggca tcttctccgc gctcatgatc
      421 ggcctcggcc gcgccgtggg cgagaccatg atcgtcgtca tggccaccgg caacaccccc
      481 gtcatggacg agaccggccg cctgctgttg ggagacgggc tgctgggcgg cggaaaattc
      541 cccctggccg cgcattggaa cccctttgac ggcatgcgca ccctctccgc caacctcgcc
      601 gtggagctgc ccgaggcccc ccagggctcc acccactacc gcaccctctt tctcggcgcc
      661 ttcgtgctct tcgtgctcac cttcgctctg aacacggcgg ccgagctgct gcgccagcgg
      721 ctgcggcgga agttcaaagt gggcagatga ggcgcaagga gaggcgcttt gatgcggaag
      781 gccttttggc cttcagaaag gcggaaggca ggctctttgt ttaccaaaag ggcacatacg
      841 tgagggtcaa ccagcacatg aacggtattg tgaccgcgac gacgattctc gtgcgcatgt
      901 gaaaaaagcg cttcaaagcc aggggggtga tcaccgcgaa accagccagg ggtgccagtg
      961 gccgccacag ctccagcaaa acgcagatga gggaaacacc aaagaccacc agcgtcgtct
     1021 tgagcaacga aaagcacacc tcatgccgag ccagcaggaa gagaagcaca gccagtgcgg
     1081 ctgcatgcat cacaacagcc ggcatcccag cctccacaat ccaaggaata gcatgattca
     1141 tggcaatgtt ttcaattcct cacttcactc ccatttgtcg ggaagtatcc ggcctttctc
     1201 gcgaaaccag gcgggaaaca tcaaaagcgg cagtccggtt agaatgataa cgatgcagag
     1261 ccattgcagg aacagggcaa gtgagactcc cgcgtcatga acgccagcca ttcgcgaacc
     1321 cgccatgagg gccacccatg tgagaatgtt ccagaaaggc tggcccgtgc gccagcccag
     1381 cgtcaaaccg gtcagaaaca gtgccgagac ggggatcacg aaaagcagct cgtgaaggga
     1441 gacgactccg tcggtgaagg tgaagagcat caccgccagc gcggccagag gcgcttgacg
     1501 aaacatttca aaagtcatcc gcgcgtcgct catggtcgtc tcaaccaggc ccagagcagc
     1561 tcccaaacga ccagccagac cagaaacaaa cccgtggccc cgaaggccgg ccccgtgcgc
     1621 agatagaaga agaacttcag cgccagtggc gtgaccacgg cgaaagccgc caggggtgcc
     1681 aggggatgca acagccccag caaaccgcag ccgaaagtga tgccaaagac cacaagcatg
     1741 gtcctgccaa aagaaagatc cgcctcatgg cgcgccagca tccagaggat ggccatgagc
     1801 gcgacggtgt tgacgatgag tccgagcatg gcgggaaggg attcggcggg gggaaatccg
     1861 caaatgtttt ggtgcgggaa gctccggatt ttcagaaacc agggcgtctg tgcttgagac
     1921 tcggcctcga tttatggaga gtaaaacccg tcgtcttgag attagaggcg tttttttgag
     1981 aattgactat tccaagcccg ctcctgggcg cgacgagccc aggggcgtct gcgggacttc
     2041 attgacctga gtgacagaac gcaagcgcgt gttggcgcgg gggcatccgc cgttttcttt
     2101 gatggcactt ggcatgaagg cgcgcatttt ccgccatgcc tgacccttcc gtgctcatcg
     2161 ttcatgcctt tttctttggc attctggttt ttctcctggc gcggacggcg ctggcggaac
     2221 gcaaatcccg caggctgctt tttgcctgca ctgtgtttgc ctggacgtgc ctgatttacc
     2281 agcttgccgg ttgggcacgg agcctgggct acaatcttca ttacagtctt gcgacttcgg
     2341 tcctgttgaa ctcgtgcgcg ggcgcgctgg aggagggaca gcacgaggac gtgacccgcg
     2401 agttgcggca actgacagat gagttccggg tcacttatga gaatcggggg gattttatgg
     2461 aactcgcgct caaggctgtg aactccttga atgggacgca ggctcaggag cagaagcaga
     2521 aggagtgacg gcgccttcgt gctcttcgcg ctcacctttg cactgaacac ggcggccgag
     2581 ctgttgcgcc agcgcctgcg gcggaggttc cgagtgggcg ggtgaggcga cggaagggcg
     2641 ggggtgaaag gcgggggtga aaggcgggag gggatggaca gcgtgacccg gaggccgggc
     2701 ttccatcctc attccgcacg gttcgcgccg gaggccatgc cgagcagata ctcatccagc
     2761 ggcgtgcctt ccggccaggt ccgcagcatg aggaaatctt cctcgccgat ctcgaaccac
     2821 gaatccacgc gccaggtcag cggcggatac tggctgacaa aacgccgtgc cgtgtgctcc
     2881 gagaccagga tgtgtgaatg aggatgcttc cgcaggattg actcaagctc ttccctgtcg
     2941 cgcgcttcga agacatcctc ccgcgtcgcg tctccgagat ggctcacgca gtcggccaga
     3001 atgaacacgt cccctccctt ttgatgcgat tccagccagc tcatgatggc ggcgcgctga
     3061 tggatttggt gggcgtccct ggcggcaagc catgcccacc ccagtgccag cgccgcagcg
     3121 gcggtgaggg aaatccacac cgggcgcaaa tgacggcccc tggattccag cttggctgcc
     3181 ttgtagtccc agggatcctg ccgccgcgcc tgcaggcagc actccgagcc cagcgcggga
     3241 aaaagcgcgc cccagagctg gttcagccgc acccaggcgg gaaaattctc cagccacccg
     3301 ccagtgcgcc gcacggcgtg gctgcccgtc acaaactccg gctccagccc ggcatccctg
     3361 gccagtgagt gccagcgccc gggggagagc accgtgatgt ggccgccggg caccaccttt
     3421 ccccgccgca ggctggcgcg cagatagccc tcgcgcaggc gcgccagcca gcccggcatc
     3481 gtcggcgtgc aacccaggaa aatgccgcca ggcttcagca cgcgggaaag ctccgccagt
     3541 gtcgctccgg gccgcggcag atgctcgaag acatgcaggc tgaccaccac gtccgcgatg
     3601 ccggaggcca ggggcagggg atcgtcgaag ttcgcgtgcc gcacctcgtc atagcaggcg
     3661 agctgtctta cttcctcacg cgggttccag tccaggccga tccagcgctc caccgcgccc
     3721 accggggtga agtgcctcag ccagcccctc tcgcagcccg cgtccaccac caccagaggc
     3781 cggcccgcac ggcgcgcctc cgcggcgatc gcgcagcccg cccaccagta gcgcagcagt
     3841 cggaaggaat aagccgcttt tcccaaatga tccgtcagct tcgtgcccgc gcctttgaca
     3901 cgcgcggcag cttcttgaaa tgagccgagg gtatattgca gaaaggtgaa tcgggagggc
     3961 attgtggtga atttttcggt caaaacctac gagtgagccg cctctgggac gctggaaaca
     4021 tggttcgcgt tgaaaattcc tgctctcatc tggaatcacc ctggcccggg ttccagttcc
     4081 atcccgcacg gtcagtgcca agctccgaac aacttccaca ggagaacggc tgcttgattc
     4141 ttgagaatt
//
LOCUS       DAOMYJ010000330         4125 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Prosthecobacter sp. isolate AMR_MDS_5629
            NODE_7668_length_4125_cov_19.713759, whole genome shotgun sequence.
ACCESSION   DAOMYJ010000330 DAOMYJ010000000
VERSION     DAOMYJ010000330.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867850
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prosthecobacter sp. (wastewater metagenome)
  ORGANISM  Prosthecobacter sp.
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Verrucomicrobiaceae; Prosthecobacter.
REFERENCE   1  (bases 1 to 4125)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4125)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.30
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:34:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,742
            CDSs (total)                      :: 4,684
            Genes (coding)                    :: 4,676
            CDSs (with protein)               :: 4,676
            Genes (RNA)                       :: 58
            tRNAs                             :: 55
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4125
                     /organism="Prosthecobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7668_length_4125_cov_19.713759"
                     /isolate="AMR_MDS_5629"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1965333"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..582
                     /locus_tag="PLP58_21145"
     CDS             <1..582
                     /locus_tag="PLP58_21145"
                     /inference="COORDINATES: protein motif:HMM:NF016906.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HRJ11101.1"
                     /translation="PGSANVRFIGKGKPYGNDETARWGHLSICDNVLSDVSVNIDLQE
                     VRGAVITGNTFGVGFEHDLLAVGCSNLAIGPNTFDRNPPYYRGKAAAVKGGLVFRDCR
                     DITLTGLHIDGVRTHPAAVQIINGDTFNISGCSILDSDGAGLLLQNTSHSLVTGCLIA
                     DRRKDRQPAPSLRVEGGEDNTFANNKLANGESK"
     gene            685..1467
                     /locus_tag="PLP58_21150"
     CDS             685..1467
                     /locus_tag="PLP58_21150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ11102.1"
                     /translation="MRLLFFAFLTLQSLHAADFHATACEGTYPRHLQGICTNGKDAIY
                     WCWTDYLVKTDTEGRILKKVPVADHHGDLCFHDGRVYVATNLAKFNRPAGEADSWVCV
                     YDAETLAELAKHRVPEVVHGAGGMAWRDGRFFIVGGLPPGVGENYVYEYDTGFRFQRR
                     HVLASGYTLMGIQTAAHASGAWWFGCYGKPQVLLRADENLRLTGKWEFNASVGIAPIS
                     GGRFLIAQNTVVKGTDGKSDGNQAKVLIARPDEKAGLVIHER"
     gene            complement(1540..2604)
                     /locus_tag="PLP58_21155"
     CDS             complement(1540..2604)
                     /locus_tag="PLP58_21155"
                     /inference="COORDINATES: protein motif:HMM:NF013847.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin-independent methionine synthase II
                     family protein"
                     /protein_id="HRJ11103.1"
                     /translation="MSKPLLFPASVVGSLPRPAFVLDLINDRPPVSAERYTHEMQAAV
                     RYAVAMQEHAGLDVVTDGEWWRKSYIGVIAELAHGFELGTAPDGRPMTVVTDKLSPKN
                     PGFIAREVTFLKTITNRLIKSTLPSPALLGERMWHPEKSAKAYPNRDDFVRDCVPILR
                     RELELVREAGAGIVQIDDPHLCLFVDHDVRGKYTDPDAAADFAVDTVNEVVSGISGVK
                     IAVHLCRRAGGRARGEVCHGGGYGPIIPHLNKLKAHHLTMEFTAPQAGDMTVFRELRE
                     DLEIGLGCVDVTPGQVDSAETIVSRVRKALEFLAPERITLNPDCGFAPGSGAAVSLDE
                     TYQKLSNEAEAARILRAEHG"
     gene            3116..>4125
                     /locus_tag="PLP58_21160"
     CDS             3116..>4125
                     /locus_tag="PLP58_21160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ11104.1"
                     /translation="MPESAAQDAPLPPPDAGARARRAQGELRVWLTAAGLAAGLVMIT
                     GLLALIAWKGLAVFWPVEVSLVRLAGAEGAGGETFAGILVKAQERRDAEGALARERQY
                     FTGNKEAGRLAFRHVDVAEIVEETRPGGIRVAERRQGGDAIFFPVALVGPDGARVEAE
                     AADFEKTLAARMAEGRAGREAGRLECRLGSGEARVLEVADLLRLHAPNDLGLAGKAGV
                     FARGVWAFLSEEPREANTEGGIFPAVFGTFVMTLLMALLVTPFGIIAAVYLREYARQG
                     LVVRVVRICVNNLAGVPSIVFGVFGLGFFVYGAGGFIDGGPEQPLPPLWWSAAVLGGL
                     GAV"
BASE COUNT          716 a         1188 c         1453 g          768 t
ORIGIN      
        1 cccggctcgg cgaatgtgcg cttcatcggc aaaggcaagc cctacggcaa cgacgaaacc
       61 gcccgctggg gccatctcag catctgcgac aacgtgctga gcgatgtctc ggtaaacatc
      121 gacctccagg aagtgcgcgg agccgtcatc accggcaaca ccttcggcgt cggcttcgag
      181 catgaccttc tcgcggtcgg gtgcagcaac ctcgccatcg gtccgaacac ctttgaccgc
      241 aatccgccct actaccgcgg caaagccgcc gcggtgaaag gcggactcgt cttccgcgac
      301 tgccgcgaca tcacccttac cggtctgcac atcgacggcg tgcgcacgca tcccgccgcc
      361 gtgcagatca tcaatggcga caccttcaac atcagcggct gctccatcct cgacagcgac
      421 ggcgcgggtc tgctgctgca aaacaccagc cacagcctcg tcaccggctg ccttattgct
      481 gatcgccgca aagaccgcca gcctgcgccg tcgctgcgag tcgaaggcgg cgaggacaac
      541 acctttgcca acaacaagct cgcgaatggc gaatccaagt agctctctcg aattgagaag
      601 ttggagtcca ggctttagcc gatctttgac ctctcaacca tcccaaaccg tcggctaaag
      661 cctggactcc aacctcacct tgccatgcgt cttctgtttt ttgctttcct cacgcttcaa
      721 tcactccatg cggcggattt ccacgcgacc gcttgcgaag gcacctatcc gcgccatctc
      781 caaggcatct gcacgaacgg caaggatgcc atctactggt gctggaccga ttatctcgtc
      841 aaaaccgaca cggagggccg catcctgaaa aaggttcccg tggcggatca tcatggcgac
      901 ctttgctttc acgatggccg cgtttatgtc gccacgaacc tggcaaaatt caaccggccc
      961 gccggcgagg cggattcctg ggtgtgtgtc tatgacgccg aaacgctggc cgagctggca
     1021 aagcatcgcg tgccggaggt cgtgcatggc gctggcggca tggcgtggcg tgatgggcgt
     1081 ttttttatcg tgggtggctt gccgcccggt gtgggggaaa actacgtcta tgaatatgac
     1141 accggcttcc gcttccagag gcgccatgtg ctcgccagcg gttacacact gatgggcatc
     1201 caaaccgccg ctcacgccag cggcgcgtgg tggttcggct gctacgggaa accgcaggtg
     1261 ctgctgcggg ccgatgagaa tctgcggctc accggcaaat gggagttcaa cgcctccgtc
     1321 ggcattgcgc ccatcagcgg cgggcggttt ctcatcgccc aaaacaccgt cgtaaaaggc
     1381 acggacggca aatcagacgg aaatcaggcg aaggtcctca ttgccagacc ggatgaaaaa
     1441 gccggccttg tcatccatga gcggtgatgc tggcctgatc gtgtcaagcc acagccaaaa
     1501 cacgcgactc ccagccatcg aagcagcggt gccagccact cacccatgct ccgcgcgcag
     1561 gatgcgggcg gcttcagcct cgttgctgag cttttggtag gtttcgtcga ggctgacggc
     1621 ggcgccggag ccaggggcga agccgcagtc gggattgagc gtgatgcgct cgggcgcgag
     1681 gaattccaag gctttgcgca cgcggctgac gatggtttcc gccgagtcca cctgcccggg
     1741 tgtgacatcc acgcaaccga gtccgatttc gagatcttca cgcagttcgc ggaagacggt
     1801 catgtcacca gcctggggcg cggtgaactc catcgtgagg tggtgggcct tgagcttgtt
     1861 caggtgcgga atgatggggc cgtagccgcc gccatggcag acctcgccac gcgcgcgtcc
     1921 accggcgcgg cggcagagat gcacggcgat tttgacgccg gaaatgccgc tgacgacctc
     1981 gttgaccgtg tccacggcga agtcggcggc ggcatcgggg tcggtgtatt tgccgcgcac
     2041 atcgtggtcc acgaagaggc acaaatgcgg atcgtcaatc tgcacgatgc cggccccggc
     2101 ctcgcgcacg agttcgagct cgcggcgcag gatgggcacg cagtcgcgca caaagtcatc
     2161 gcggttcgga taggccttgg cggacttctc cgggtgccac atgcgctcgc cgagcagcgc
     2221 gggcgagggc agcgtggact tgatgaggcg gttggtgatc gttttgagaa acgtgacctc
     2281 gcgggcgatg aagccggggt ttttgggtga gagcttgtcc gtgacgacgg tcatgggccg
     2341 gccatccggc gcggtgccga gttcaaagcc gtgcgccagt tccgcgatga cgccgatgta
     2401 gcttttgcgc caccattcgc cgtcggtgac gacatccagc cccgcgtgct cctgcatggc
     2461 gacggcgtag cgcacggcgg cctgcatctc gtgcgtgtag cgctcggcgg agacaggcgg
     2521 gcggtcgttg atgagatcaa gcacgaaggc cgggcgcggc agcgagccga cgacggaggc
     2581 ggggaagaga agaggcttgc tcatgaatga tgcggtgctg gacagtgaag tgaggggtcg
     2641 aagttggaca acgaaacgtt ctgtgcggaa atgatgaaat gggatgcatt accaacggtc
     2701 aattcaatga gcagcttcaa gctcaatttc accccttatg agaacttcaa aacccaagcc
     2761 tttcctccac tccaaatcgc ctaaccttgc ggcaaatacc tctttctcag ggatttaggc
     2821 aacttttccc atgacggaga caactgtttc cgtgacggag acaactgttt ccgtggcgga
     2881 gacaactgtt tccgtgacgg aaacaactgt ttcgatgacg cagacaactg tttccgtggt
     2941 gaaaactacg gccttaatga gaaagacctt ggtttgatga gactcaagcg tttcccgatg
     3001 cctggatgcc ggggcttggc aagcccggct ccctgcggca agccggctgc gggtcgagaa
     3061 tgtgggttgc ttttggaccg gggcgggcat ggtggcggcg gtttcatttc tcgccatgcc
     3121 ggagtctgcc gcccaggatg ccccgctgcc gccgcccgat gctggcgcgc gggcacggcg
     3181 cgcccagggg gagctgcggg tgtggctgac ggcggcgggt ctggcggcgg ggttggtgat
     3241 gatcacgggc ctgctggcgc tcatcgcgtg gaaggggctg gcggttttct ggccggtgga
     3301 ggtgagcctg gtgcggctgg cgggggcgga gggcgcgggg ggggagactt tcgcgggcat
     3361 cctggtgaag gcgcaggagc ggcgggacgc ggagggcgcg ctggcgcggg agcggcagta
     3421 tttcacgggg aacaaggagg cgggccggct ggccttccgc catgtggatg tggcggagat
     3481 cgtggaggag acaaggccgg gcggcatccg cgtggcggag cggcggcagg gcggggatgc
     3541 gatctttttt cccgtggcgc tggtggggcc ggatggggcg cgggtggagg cggaggcggc
     3601 ggattttgaa aaaacgctgg cggcgcggat ggcggagggg cgcgcgggcc gggaggcggg
     3661 gcggctggag tgccggctgg gctcggggga ggcgcgggtg ctggaggtgg cggacctgct
     3721 gcgcctgcat gcgccgaatg acctggggct ggcgggaaag gcgggggtgt tcgcgcgcgg
     3781 ggtgtgggct tttctctcgg aggagccgcg ggaggcgaac acggaggggg ggatttttcc
     3841 ggcggtgttc ggcacgttcg tgatgacgct gctgatggcg ctgctggtga cgccgttcgg
     3901 catcatcgcg gcggtgtatc tccgggagta tgcgcggcag ggactggtgg tgcgggtggt
     3961 gcgcatctgt gtgaacaatc tggcgggcgt gccctcgatc gtgttcgggg tgttcgggct
     4021 gggcttcttc gtctatgggg cgggtggttt cattgacggc gggccggagc agccgctgcc
     4081 gccgctgtgg tggagcgcgg cggtgctggg cggtctgggc gcggt
//
LOCUS       DAOMYJ010000331         4116 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Prosthecobacter sp. isolate AMR_MDS_5629
            NODE_7690_length_4116_cov_19.764344, whole genome shotgun sequence.
ACCESSION   DAOMYJ010000331 DAOMYJ010000000
VERSION     DAOMYJ010000331.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867850
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prosthecobacter sp. (wastewater metagenome)
  ORGANISM  Prosthecobacter sp.
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Verrucomicrobiaceae; Prosthecobacter.
REFERENCE   1  (bases 1 to 4116)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4116)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.30
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:34:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,742
            CDSs (total)                      :: 4,684
            Genes (coding)                    :: 4,676
            CDSs (with protein)               :: 4,676
            Genes (RNA)                       :: 58
            tRNAs                             :: 55
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4116
                     /organism="Prosthecobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7690_length_4116_cov_19.764344"
                     /isolate="AMR_MDS_5629"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1965333"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..270)
                     /locus_tag="PLP58_21165"
     CDS             complement(<1..270)
                     /locus_tag="PLP58_21165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ11105.1"
                     /translation="MRLIHFAQALTLILLAITGYLAWEGQQAARGAKRELEHLKKVQA
                     AEEAAKPAGPQALPVIPPVASITPPAPGALAQSEEPGLLPGPGALP"
     gene            complement(318..1367)
                     /locus_tag="PLP58_21170"
     CDS             complement(318..1367)
                     /locus_tag="PLP58_21170"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018970491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-dihydrouridine synthase family protein"
                     /protein_id="HRJ11106.1"
                     /translation="MRDLLPTQRPGLLLAPMQDVTDLPFMRLMARYGGPDLFVTEYFR
                     VTRDSKPEPQILRSITENETGIPVMAQIIGQDVEAMVRTARALERHPVAGIDLNLGCP
                     APIVCRKHAGGGLLRHPERVDALVGALRQAVDGRFTVKCRAGFDSPDEFPRLLEIFQK
                     HALDGLTIHARTVREGYKTAVHPECVAAAVRSLPFPVIANGNVVNARTGLAYLRRTGA
                     AGLMIGRGAIRHPWIFDELRASFSGAEAPGPRGRDLLGYIRELYEITAAFHPVFHELR
                     HVQKMKRYLGYIAGGVDREFEHHIRRVVTPAEFDAVCRRFLDHDEPLPCEPPHDSPVF
                     CGFRDLTVTEAEPVS"
     gene            1471..>4116
                     /locus_tag="PLP58_21175"
     CDS             1471..>4116
                     /locus_tag="PLP58_21175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009963474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1080 domain-containing protein"
                     /protein_id="HRJ11107.1"
                     /translation="MIRRLLLLPFFLLTPALAAPVPLFDGKTFAGWEGDTEKTWRIEE
                     GVILGGSMEGNPQNEFLATTKEYRNFILRFEYKLTGTEGFVNGGVQFRSQRVSEPPNE
                     MIGYQADIGAGYSGHLYDESRRKTMLAKPDKLQIESIEKPGEWNRYEIRAEDQRIQLR
                     LNGTRTVDYTEREPGIPLKGRIALQIHGNCKAVIAFRKIMIEELPDALVPEGAQVQER
                     FGNPESAAVVPAPFQDGRFEIAQGEVIVLAGQENLVREQKAGALEAILTHELAERQPR
                     FRSMAWEGDTVYEQWRDLNFGDWQAQLAAVGAGMVICQFGQVEAFDGKARVAEFTSAY
                     HRLLDQFRARTPRLVLISPMPFEKPDAAHAPDAPDLTLRNDDVKAYADAVREIARQRG
                     ALFVDLFTELSRRSPQAPRLTDDGLHLNAEGLRVVGRIIGQQLGVSSSDSDDLAALRE
                     AIVEKNRLWFDCWRPANWSFVYGDRVTQMFGKPAADALSLRESFEAHKPLIAWHDALI
                     HATAQGKPHATPKPAPVQPTQAAPEPVLTPEQQIASFRVAEGYEMSLFASEAQGVMKP
                     VQFAWDEKGRLWVACSPTYPHTLPGVKPSDFILVLEDTDHDGIADKSTRFAEGLTMVQ
                     GVEPGAGGAYVCDFDRLIHLRDTDGDGRADQTRVLFSGFGIGDTHQLINSICHGPDGA
                     LWFTQGLHAFSRVETPHGLAILEKAGVWRLDPRTLKLEGFFNGGKAGHNCWGVAFDDH
                     FQVFHKSGDRPAGYFSTPGLIPLREPDEYHPVGQLFDTSPKTNSLEIIGTRALPEEIQ
                     GCALIGGYFGSVVELHRFEDEGSGFKTTQLPRLVTSTDPSFRPVDVSIGPDGAMYLCD
                     WFNPVIGHYQASYADPRRDRAHGRIW"
BASE COUNT          695 a         1321 c         1419 g          681 t
ORIGIN      
        1 aggcagggcg ccggggccgg gcaaaaggcc gggctcctcg ctttgcgcca gcgcacccgg
       61 cgcgggcggg gtgatgctgg ccacgggcgg aatgacgggc agggcctgtg ggccggcggg
      121 tttcgcggcc tcctcggcgg cctggacttt tttcaggtgc tccagctccc tctttgctcc
      181 gcgcgctgcc tgctggccct cccaggccag gtagccggtg atggcgagca ggataagggt
      241 gagggcttgg gcaaagtgga tcaatctcat ggcgcggacg ctagcaggct gtgccgcgcc
      301 gcgcaacggg cttggcatca cgagacgggc tccgcctccg ttacggtgag atcgcggaag
      361 ccgcaaaaga ccggcgagtc gtgcggcggc tcgcagggca gcggctcgtc gtgatcgaga
      421 aaacggcggc acaccgcgtc gaactcggcg ggtgtcacca ccctgcggat gtgatgctcg
      481 aactcgcgat ccaccccgcc cgcgatgtag ccgaggtagc gcttcatctt ctgcacatgc
      541 ctcagctcat ggaagaccgg atgaaaagcg gcggtgatct cataaagctc gcggatgtag
      601 ccgagcaagt cccgcccgcg cggtcccggc gcctcagcgc cggaaaagga ggcgcgcaat
      661 tcgtcgaaaa tccacggatg gcggatcgcg ccgcgcccga tcatgagacc cgccgcgccc
      721 gtgcggcgca ggtaggccag gccggtgcgg gcattgacca cgttgccgtt ggcgatgacc
      781 ggaaaaggca gcgagcgcac ggctgcggcc acgcattcag gatgcaccgc ggttttgtaa
      841 ccctcgcgca ccgtccgggc gtggatcgtg aggccgtcga gcgcgtgctt ttggaagatc
      901 tccagcagcc ggggaaactc gtccggactg tcaaaaccgg cgcggcactt cacggtgaag
      961 cgcccgtcca cggcctggcg cagcgctccg acgagcgcgt ccacacgctc cgggtggcgc
     1021 agcaggccgc ctcccgcgtg cttgcggcag acaatgggcg ccgggcagcc cagattgagg
     1081 tcaatgccgg ccacagggtg cctctccagc gcgcgggcgg tgcggaccat ggcctccaca
     1141 tcctggccga tgatctgcgc catgacggga atgccggtct cgttctccgt gatggagcgc
     1201 aggatttgcg gttcgggctt cgagtcccgc gtgacacgga aatactccgt gacaaaaagg
     1261 tccggcccgc cataccgcgc catcaggcgc atgaatggca ggtcggtcac gtcctgcatc
     1321 ggggcgagca ggaggccggg gcgctgtgtg ggcaggaggt ctcgcacgat gtggagcctt
     1381 gaaggcttcc gccaaaactt gcaaggacac atgcgggttt cctgatttca ttcccggccc
     1441 tgccgcgtta cctcacgctc tttttccgcc atgatccgcc gactgctcct tctgcctttc
     1501 tttctcctta ctcccgccct ggccgctcca gtgccgttgt ttgacggcaa aaccttcgcg
     1561 ggctgggagg gcgacacgga gaagacgtgg cgcatcgagg agggtgtcat tctcggcggc
     1621 tccatggagg ggaatccgca aaatgagttt ctggcgacga cgaaggagta caggaacttc
     1681 atcctgcgct tcgagtacaa gctcacgggc acggagggtt ttgtgaacgg cggggtgcag
     1741 ttccgcagcc agcgcgtcag cgagccgccg aatgagatga tcggctatca ggcggacatc
     1801 ggcgcgggtt actcggggca tttgtatgac gagtcccggc gcaagacgat gctggcgaag
     1861 ccggacaagc tgcagatcga gagcatcgag aaaccgggcg agtggaaccg ctatgagatc
     1921 cgcgccgagg accagcgcat ccagctccgg ctcaatggca cgcgcacggt ggactacacg
     1981 gagcgggagc cgggcatccc tttgaaaggc cgcatcgctt tgcaaatcca cggcaactgc
     2041 aaggcggtga tcgcttttcg gaagatcatg atcgaggagc tgccggacgc gctggtgccg
     2101 gagggggcgc aggtgcagga gcgctttggc aatcccgagt cggcggcggt ggtgcccgcg
     2161 ccttttcaag acgggcgctt cgagatcgcg cagggggagg tgattgtcct ggcagggcag
     2221 gagaatctgg tgcgggagca gaaggcgggc gcgctggagg ccatcctcac ccacgagctg
     2281 gcggagcggc agccgcgctt ccgcagcatg gcctgggagg gggacacggt gtatgagcag
     2341 tggcgggatt tgaatttcgg cgactggcag gcgcagctcg cggcggtggg cgcgggcatg
     2401 gtgatctgcc agttcggcca ggtggaggcc ttcgatggca aggcgcgcgt ggcggagttc
     2461 acctcggcgt atcaccggct gctggaccag ttccgcgcgc gcacgccgcg gctggtgctc
     2521 atctcgccga tgccttttga aaagccggac gcggcccatg cgccggacgc gccggacctg
     2581 actttgagaa atgacgacgt gaaggcctat gccgacgccg tgcgggagat cgcccggcag
     2641 cgtggcgcgc tgtttgtgga tcttttcacc gagctctccc gtcgcagtcc gcaggcgccg
     2701 cgcctcacgg atgacggcct gcatttgaat gccgagggtc tgcgcgtggt ggggcgcatc
     2761 atcggccagc agctcggggt ttcctccagt gactcggatg acctggccgc gctgcgggag
     2821 gccattgtgg aaaagaaccg cctgtggttc gactgctggc ggcccgcgaa ctggtccttc
     2881 gtctatggcg accgcgtgac gcagatgttc ggcaagcccg ccgccgacgc gctctccctg
     2941 cgcgagagct tcgaggcgca caagccgctc atcgcctggc atgacgcgct catccacgcc
     3001 accgcgcagg gcaagccgca cgccacgccg aaacccgcgc cggtgcagcc gacgcaggcc
     3061 gcgccggagc ctgtgctgac gccggagcag cagatcgcca gcttcagggt ggccgagggg
     3121 tatgagatga gccttttcgc ctccgaggcg cagggtgtga tgaagccggt gcagttcgcc
     3181 tgggatgaaa aaggccgcct ctgggtggcc tgctccccca cctacccgca cacgctgccg
     3241 ggtgtgaagc cctccgactt catcctcgtg ctggaggaca cggaccacga cggcatcgct
     3301 gataaaagca cccgcttcgc cgaggggctc accatggtgc agggcgtgga gcccggcgcg
     3361 ggcggtgcgt atgtgtgcga cttcgaccgc ctcatccatc tgcgcgacac ggacggcgac
     3421 ggccgcgccg accagacccg cgtgcttttc tccggctttg gcattggcga cacgcaccag
     3481 ctcatcaact ccatctgcca cggcccggac ggcgcgctgt ggttcacgca gggcctgcac
     3541 gccttctcgc gtgtggagac gccgcacggc ctggccatcc tggaaaaagc cggcgtctgg
     3601 cggctggacc cgcgcacgct gaagctggag ggctttttca atggcggcaa agccgggcac
     3661 aactgctggg gcgtggcctt tgacgaccac ttccaggtct tccacaaaag cggcgaccgc
     3721 cctgcgggtt acttcagcac gccgggcctc atccccctgc gcgagccgga tgaataccac
     3781 ccggtgggcc agctcttcga caccagcccg aagacgaact cgctggagat catcggcacg
     3841 cgcgcgctgc cggaggaaat ccagggctgc gcgctcatcg gcggctactt cggcagcgtt
     3901 gtggagctgc accgcttcga ggacgaaggc agcggcttca agaccacgca actgccccgc
     3961 ctggtcacca gcacggaccc ttctttccgc cccgtggatg tcagcatcgg cccggatggc
     4021 gccatgtatt tgtgtgattg gttcaacccc gtcatcggac actaccaggc cagttacgcc
     4081 gacccgcggc gcgaccgcgc ccacggccgc atctgg
//
LOCUS       DAOMYJ010000332         4094 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Prosthecobacter sp. isolate AMR_MDS_5629
            NODE_7745_length_4094_cov_16.320624, whole genome shotgun sequence.
ACCESSION   DAOMYJ010000332 DAOMYJ010000000
VERSION     DAOMYJ010000332.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867850
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prosthecobacter sp. (wastewater metagenome)
  ORGANISM  Prosthecobacter sp.
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Verrucomicrobiaceae; Prosthecobacter.
REFERENCE   1  (bases 1 to 4094)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4094)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.30
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:34:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,742
            CDSs (total)                      :: 4,684
            Genes (coding)                    :: 4,676
            CDSs (with protein)               :: 4,676
            Genes (RNA)                       :: 58
            tRNAs                             :: 55
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4094
                     /organism="Prosthecobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7745_length_4094_cov_16.320624"
                     /isolate="AMR_MDS_5629"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1965333"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1120)
                     /locus_tag="PLP58_21180"
     CDS             complement(<1..1120)
                     /locus_tag="PLP58_21180"
                     /inference="COORDINATES: protein motif:HMM:NF025056.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003953 - NAD+ nucleosidase activity
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="toll/interleukin-1 receptor domain-containing
                     protein"
                     /protein_id="HRJ11108.1"
                     /translation="MAYELFISYSRKDNIPRPLPPDSPSAIPNPPSKGWVTALHDEIL
                     ADHRRFSTEPLRIFFDTSEIKDMDDWRHRILTGLRESRILLVCLSPDYFKSPPCLWEW
                     EEYLKRQVHALMGQESIAPVYFVEAPDSDEQLNAAWAEAVTRRVVTPADRPVWEQRWK
                     AWHEGTLGRPNWVDLKPWFPHGTEALREAAVREKLAALGTSLWERIQRARRATGAPGN
                     LRRLNPHFIGRQTELRQLHENLALGAVGVVTAVHGLGGQGKTELATAYAHGWADSYPA
                     GLWVLGAEGRKEILPLLGELCAGLEIPLATGPDETAEARGRRVLAELKRRAFEAKDRD
                     PDKGAATLVLLDNVSEPALLARFELEHGLQTNRGHRLHC"
     gene            complement(1365..2672)
                     /locus_tag="PLP58_21185"
     CDS             complement(1365..2672)
                     /locus_tag="PLP58_21185"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009964337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070573 - metallodipeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane dipeptidase"
                     /protein_id="HRJ11109.1"
                     /translation="MQKLPLAALAGDVNIHVRAQSPREIWRTGNEAIDGPREVALNLL
                     KPAQAQIERAWELHFGSVVFESYGFAPRCAIDGERFNEAVREGASADELGDLRESMSM
                     NGHTRSEREKTEFFEAFRAAGVTCIFQNAGEEGSDPMRLLKRLAHFTQAVDLQPDLVK
                     VLRADDVPEVKKAGRVSLCFTGNGVPLRQEWKSVRDELRMIRIFHELGIRMMHVTYNR
                     RNPLGDGAGEPHDGGLSDFGRAAVAEMNRAGVIVDVAHSGWKTSLDAAKASAKPMVAS
                     HSACADVYKHFRSKPDEVIKAVCDTGGYIGICCISRFLGGRGDITSMMDHLDHVLKKF
                     GPDHAAIGVDTAYTSRFDKEERARVLRRPDGSPAIGGSVDRWEHLWPQDDFKTTLEAE
                     RSIAWSNWPLFTLGMVMRGHSDDAIRRVLGGNVMRVLKANETV"
     gene            complement(2814..3608)
                     /locus_tag="PLP58_21190"
     CDS             complement(2814..3608)
                     /locus_tag="PLP58_21190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009965877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HRJ11110.1"
                     /translation="MNGLHLTFLGTGTSVGIPMIGCDCATCSSTDPRDNRLRSSVWMR
                     APEAAWVVDTGPDFRTQCLRAGIRHLDAALYTHPHMDHVTGFDELRRFTPGTDDAIPI
                     HALPSTLAVLERMFDYAFNGENRYRGYLKPFPKPVHGPFHLGETRITPLPVRHGKVET
                     TGYLFTRSARKLCAYIPDAKELLPETLAALQGVDTLILDALRHTQHPTHLTFAESLAI
                     HQILRPRQTWFTHLQCEIMHSIEEPLLPGGVRIAYDGLELHWPDPD"
     gene            complement(3653..>4094)
                     /locus_tag="PLP58_21195"
     CDS             complement(3653..>4094)
                     /locus_tag="PLP58_21195"
                     /inference="COORDINATES: protein motif:HMM:NF014500.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ11111.1"
                     /translation="GRCVQEIIEVSGYFGSALRQTGVAELIQQMGGTAARHWALPICF
                     LVTALVRTAQGSATVAMITAAPIAKAFLDSGDLGFHPVYLALAVGCGSKPVPWFNDSG
                     FWIITRMTGLHEGQTLRIVTPMMSLMGLAGLPVVMLGAWLWPMV"
BASE COUNT          651 a         1207 c         1544 g          692 t
ORIGIN      
        1 tgcagtgtaa cctatgtccc cggtttgttt gtaacccatg ttccagttca aaccgggcca
       61 gcagggcggg ttcggagacg ttgtcgagga ggacgagggt ggcggcgccc ttgtcgggat
      121 cgcggtcttt ggcttcgaac gcgcggcgtt tcagctcggc gaggacgcgg cgtccgcggg
      181 cctcggcggt ttcgtcgggg ccggtggcga gggggatttc cagcccggcg cagagttcgc
      241 cgagcagggg gaggatttcc tttcgtccct ccgcgccgag cacccagagg ccggcgggat
      301 aggaatcggc ccagccgtgg gcgtaggcgg tggcgagttc ggttttgccc tggccgccga
      361 ggccatgcac ggcggtgacg acgccgacgg cgccgagggc gaggttttcg tgaagctggc
      421 gcagctcggt ctggcggccg atgaagtggg ggttgaggcg gcggagattg ccgggcgcac
      481 cggtggcgcg gcgggcgcgc tggatgcgct cccacaggct ggtgccgagg gcggcgagct
      541 tttcgcgcac ggcggcctcg cgcagggcct cggtgccgtg gggaaaccaa ggcttgagat
      601 ccacccagtt cgggcggccg agcgtgcctt cgtgccaggc cttccaccgc tgctcccaga
      661 ccgggcggtc ggcgggtgtg acgacccggc gcgtgacggc ctcggcccac gcggcgttga
      721 gttgctcgtc gctgtcgggc gcttccacga aatacacggg cgcgatgctc tcctggccca
      781 tgagggcgtg gacctggcgc ttgagatact cctcccattc ccagaggcag ggcggacttt
      841 tgaagtaatc cggcgagagg cagacgagga gaatccggct ctcacgcagg ccggtgagga
      901 tgcggtggcg ccagtcgtcc atgtctttga tctcggacgt gtcgaagaaa atgcgcagcg
      961 gctcggtgga gaagcggcgg tggtcggcga ggatttcgtc gtggagggcg gtgacccagc
     1021 ccttggatgg cggattgggg attgcggatg gcgaatcggg cggcagaggg cgcgggatgt
     1081 tgtctttccg cgagtaggag atgaaaagct cgtaggccat ggcgggcgga ggttagggag
     1141 gcgggcgcgg cggcggcaag atttatttgc gccagcccct tcgaaaaaca gggcgtttcc
     1201 atgcgatgcc acaagctgcc gagtgcgggc ggcatgggaa aacacgcgct tcacaagcta
     1261 aagcttgaac tccaacgagc tgcgcttgcg catgaaaacg ccctgcttcg gaaaagcggc
     1321 atcacgcgcg tccttcctcc ccgcgaccgg gaccgcgcgg cggctcaaac ggtctcgttg
     1381 gccttgagca cgcgcatgac attgccgccg agcacccggc ggatggcgtc atcactgtgg
     1441 ccgcgcatga ccatgccgag ggtgaaaagc ggccagttgc tccaggcgat gctgcgctct
     1501 gcctcaagcg tggtcttgaa gtcatcctgc ggccacagat gctcccagcg gtccaccgag
     1561 ccgccgatgg ccgggctgcc gtccgggcgg cgcagcaccc tggcgcgctc ctccttgtca
     1621 aagcgggagg tgtaggccgt gtccacgccg atggcggcgt gatccgggcc gaactttttg
     1681 agcacatggt ccaggtgatc catcatggag gtgatgtccc ccctgccgcc gaggaagcgg
     1741 gagatgcagc agatgccgat gtagccgccg gtgtcgcaca cggccttgat gacctcgtcg
     1801 ggcttggagc ggaagtgctt gtagacatcc gcgcaggcgc tgtggctggc gaccatgggc
     1861 ttggcggagg ccttggcggc gtcgaggctg gtcttccagc cgctgtgcgc cacgtctacg
     1921 atgacaccgg cgcggttcat ctccgccacc gccgcgcggc cgaagtcgct gaggccgccg
     1981 tcgtgcggct cgcccgcgcc gtcgcccagc ggattgcggc ggttgtaagt cacatgcatc
     2041 atgcggatgc ccagctcgtg gaagatgcgg atcatgcgca gctcgtcgcg cacgcttttc
     2101 cactcctggc gcaggggcac gccgttgccg gtgaagcaga ggctcaccct gccagccttc
     2161 ttcacctccg gcacgtcgtc ggcgcgcagc accttcacca ggtccggctg caaatccacc
     2221 gcctgggtga agtgcgccag ccgcttgagc aggcgcatgg ggtcgctgcc ctcctcgccg
     2281 gcgttttgga agatgcaggt gactccggcg gcgcggaagg cctcgaagaa ctccgtcttc
     2341 tcccgctcgc tgcgcgtgtg gccgttcatg gacatggact cgcgcaggtc gcccagctcg
     2401 tccgccgacg cgccctccct caccgcctcg ttgaagcgct ccccgtcaat cgcgcagcgc
     2461 ggggcgaagc cgtaggactc gaagaccacc gagccgaagt gcagctccca cgcgcgctcg
     2521 atctgcgcct gcgcgggctt gagaaggttc agcgcgacct cacgcggccc gtctatcgcc
     2581 tcattgcccg tgcgccagat ctcgcgcggc gactgcgccc tgacatgaat gttcacgtcc
     2641 cccgccagag cggcgagagg cagcttttgc aaaagggagc ggcgggagaa tccaggagac
     2701 gacatccgaa accaacggcg cgggaaagcg gatttccaaa gcgggctgcc aaaactttgg
     2761 cggtgttcat cctgcccggt ggttttggcc gggcttgcag ccgtgccatt gactcaatcc
     2821 gggtcgggcc agtggagttc caggccgtcg taagcgatgc ggactccccc gggcaggagg
     2881 ggttcctcga tggagtgcat gatctcgcat tgcaggtggg tgaaccaggt ctggcgcggg
     2941 cgcaggattt ggtggatggc gagggattcg gcaaaggtga gatgcgtggg atgctgggtg
     3001 tggcgcaggg cgtcgaggat gagggtgtcc acgccctgga gggcggcgag ggtttcgggc
     3061 agcagctctt tggcgtcggg gatgtaggcg cagagcttgc gcgcgctgcg ggtgaaaaga
     3121 tagccggtgg tctcgacctt gccgtggcgg acaggcagcg gggtgatgcg cgtttcgccg
     3181 aggtgaaagg ggccgtggac gggtttggga aagggcttca aatagccgcg gtagcggttt
     3241 tcgccgttga aggcgtagtc gaacatgcgt tcgaggacgg cgagggtgga gggcagggcg
     3301 tggatgggga tggcgtcgtc ggtgccgggg gtgaagcggc gcagctcgtc gaagccggtg
     3361 acgtggtcca tgtgcgggtg ggtgtagagg gcggcatcca ggtggcggat gccggcgcgc
     3421 aggcactggg tgcggaagtc ggggccggtg tccacgaccc aggcggcctc gggcgcgcgc
     3481 atccacacgg aggagcgcag gcggttgtcg cgcgggtcgg tgctgctgca ggtggcgcag
     3541 tcgcagccga tcatcgggat gccgacggag gtgccggtgc cgaggaaggt gagatggagg
     3601 ccgttcatgc gcggaggagt gagtatctcc atgacagggc ggcggatttt tgtcaaacca
     3661 tgggccaaag ccaggcgccg agcatgacga cgggcagccc ggcgagcccc atgagggaca
     3721 tcatgggcgt gacgatgcgc agcgtctggc cctcgtgcag gccagtcatg cgggtgatga
     3781 tccagaagcc gctgtcattg aaccagggca cgggctttga cccgcagccg acggcgagcg
     3841 cgagatagac ggggtgaaag ccgaggtcgc ccgagtcgag aaaggctttg gcgatgggcg
     3901 cggcggtgat catggcgacg gtggcggagc cctgcgcggt gcgcaccagg gcggtgacga
     3961 gaaagcagat gggcagcgcc caatgccgcg cggccgtgcc gcccatctgc tggatgagct
     4021 cggccacgcc ggtctggcgc agggcgctgc cgaagtaccc ggaaacctcg atgatttcct
     4081 ggacgcagcg gcct
//
LOCUS       DAOMYJ010000333         4093 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Prosthecobacter sp. isolate AMR_MDS_5629
            NODE_7748_length_4093_cov_20.878158, whole genome shotgun sequence.
ACCESSION   DAOMYJ010000333 DAOMYJ010000000
VERSION     DAOMYJ010000333.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867850
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prosthecobacter sp. (wastewater metagenome)
  ORGANISM  Prosthecobacter sp.
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Verrucomicrobiaceae; Prosthecobacter.
REFERENCE   1  (bases 1 to 4093)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4093)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.30
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:34:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,742
            CDSs (total)                      :: 4,684
            Genes (coding)                    :: 4,676
            CDSs (with protein)               :: 4,676
            Genes (RNA)                       :: 58
            tRNAs                             :: 55
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4093
                     /organism="Prosthecobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7748_length_4093_cov_20.878158"
                     /isolate="AMR_MDS_5629"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1965333"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..472
                     /locus_tag="PLP58_21200"
     CDS             <1..472
                     /locus_tag="PLP58_21200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ11112.1"
                     /translation="LAAAAVVFWMPRGNGVLSFAEAEVELVKMFDRMQTEGFSLDHVS
                     DELAEVGTWLADAGAPLPYVVHAGARAATPMGCRVLDWRGGRVTMICFRKGGDSAHLF
                     VLDRARLAEGEGPLSRDPAPVHHVDGRPVTAWGCRKCLYLLVGGAKDTRLDDFL"
     gene            521..3649
                     /locus_tag="PLP58_21205"
     CDS             521..3649
                     /locus_tag="PLP58_21205"
                     /inference="COORDINATES: protein motif:HMM:TIGR02604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDSL-type esterase/lipase family protein"
                     /protein_id="HRJ11113.1"
                     /translation="MNRLVFLLAFITAGASAQLPLELKPGTRIALIGNSLFDRMRDDG
                     QFEALMHQRFAREKLVFRNLSWSADEVALRPRPDGFGDLNQHLAEHKADVILAAFGFN
                     ESFKGEKGLAEFETLLKAFLIELKAHRYNGTSEPKIVLVSPTAAEKPHEHLNAQIKLY
                     SDAMKKVAAAEKVAFADVFDIPPSEFRTPHSTNGVHLNQEGYRVFSERLYNQLTQETP
                     PKLREVVRAAVVEKEDKFFQHYRPLNYYYIKGGRMEPYGVVNFPGELKKLLQMTEARD
                     QLIWTTASTDFADGADSKTKIQSEKSVKSVDETPLPVITGDRPINEWLSPADELKAFK
                     IDPRFEVSLFASEEDFPDLFVKPIAIRFDGKGRLWVSTSTTYPQIMPGEAPQDRILIL
                     EDTNGDHRADKCSVWADKLAIPLSFEFGNGGVFVSDQPHLTFLKDTDGDGKADHREKL
                     LTGFGTEDSHHALHDFVWSPEGDLIFRESIFHHSQVETPYGPVRARESSFFRYTPATG
                     KLLAFGSYMSTNPWGITFDDWGFHVGSHPVFASAVHALNAPYPDIHIPAGNYFPAYSG
                     TCGQEFLTSSHWPKDLRDKKHFMRVRYKPTNEVELHEWVEHDTHFEEKKVGIVFQSTN
                     LSFIPVDIQQGPDGAMYIADWYNPVKGHMQYSLRDTRRDKKSGRIWRITAKDQPLEEA
                     PKIAGASVPELLELLKSENYRTRYRAKVELREREPEEITVGIGLSGRFHLNTDQALAE
                     WLWLNDWLVKQNFENGITPEISKAETWDFSNHMLSRIMREHDKFNHLMRAVAARSLRK
                     LPLREWKEIVGPQPLRIAANDPSGLVRLEAAIAASYIGTPEALEAALDLLKHPMDPYL
                     TYALRTSLDSHTLQPLWKGNTAFMAKHPELVKFLEDSEPKQPARMAKKKKPEAPHPFD
                     AQPGLQTVTIKTIPERLLYDVRDFKVKAGQPVKLVFENPDVTPHNLLIVQPGAADEVG
                     MAGNEMAKLPDGMAKGFLPDSPKILHHTRMLMQNESETLRFTAPEMAGAYPYICTFPG
                     HWLVMKGEMVVE"
     gene            3692..>4093
                     /locus_tag="PLP58_21210"
     CDS             3692..>4093
                     /locus_tag="PLP58_21210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009959137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HRJ11114.1"
                     /translation="MSLFPSEAARLEAFPVARDSIFLAHAGVTILPRVVARTMQDYLE
                     QCSLLMQEYPEAWRAVNETRVTAARLIGAKAREISLLGPTSVGLSLVANGLDWQPGDE
                     VVCYSDDYPANVYPWTGLARRGVTVRLLRPEE"
BASE COUNT          849 a         1411 c         1142 g          691 t
ORIGIN      
        1 gcttgcggca gccgccgtgg tcttctggat gccgcgcgga aacggcgtcc tctcttttgc
       61 cgaggcggag gtggagctgg tgaaaatgtt cgaccgcatg cagacggagg gtttttccct
      121 ggatcatgtc agcgacgagc tcgccgaggt cgggacctgg ctggcggatg ccggggcgcc
      181 gctgccctat gtggtccatg ccggcgcccg cgccgccacg cccatgggct gccgcgtgct
      241 tgattggcgc ggcggcaggg tcaccatgat ctgcttccgc aaaggcggcg actccgccca
      301 cttgtttgtc ctggaccgcg ccaggctcgc cgagggcgag gggccgctga gccgtgatcc
      361 cgcgcccgtt catcatgtgg atggccgtcc cgtcaccgct tggggctgcc gcaaatgcct
      421 ctaccttctc gtcggcggcg ccaaggacac acggctggac gatttcctgt gacgcggcag
      481 gcgggcgcga gtttgagcgg aagggcgcgt tctttccgcc atgaaccgtc tcgtctttct
      541 cctcgcgttc atcacagccg gcgcctccgc ccagctcccg ctggaattga agccaggcac
      601 gcgcatcgct ctcatcggga actcgctctt cgaccgcatg cgcgacgacg gccagttcga
      661 ggcgctgatg caccagcgct ttgccaggga gaagctcgtt ttccgcaatc tgagctggtc
      721 cgccgatgaa gtggccctgc gcccgcgccc cgacggcttt ggcgacctca accagcacct
      781 cgccgagcac aaggccgacg tcatcctcgc cgccttcggc ttcaacgaga gcttcaaagg
      841 cgaaaaaggc ctcgccgagt tcgaaacact cctcaaagcc ttcctcatcg agttgaaagc
      901 gcatcgctac aacggcacct ccgagccgaa aatcgtcctc gtgagcccca ccgccgccga
      961 aaagccgcat gagcatctca acgcgcaaat caagctctac tccgacgcga tgaaaaaggt
     1021 cgctgcggcc gagaaagtgg ccttcgcgga tgtgttcgac attccgcctt ccgaattccg
     1081 cactccgcat tccaccaatg gcgtccacct caaccaggaa ggctaccgcg ttttctccga
     1141 gcgcctctac aaccaactca cccaagaaac cccacccaag ctccgcgaag tcgtccgcgc
     1201 cgccgtcgtc gaaaaggagg acaagttctt ccagcattac cgcccgctga actactacta
     1261 catcaaaggc ggccgcatgg agccctacgg cgtcgtgaac ttccccggcg agctgaaaaa
     1321 actcctccag atgaccgaag cacgcgatca actcatctgg accaccgcat ccacagattt
     1381 cgcagatggc gcagattcaa aaaccaaaat ccaatctgag aaatcggtga aatcggtgga
     1441 tgaaactccc cttcccgtga tcaccggaga ccgccccatc aacgaatggc tctctcccgc
     1501 tgatgaactg aaagccttca aaatcgaccc gcgcttcgaa gtcagcctct tcgccagcga
     1561 agaagacttc cccgacctct tcgtgaagcc catcgccatt cgtttcgatg gcaaaggccg
     1621 cctgtgggtc agcacgagca ccacctaccc gcaaatcatg ccaggcgagg ccccgcagga
     1681 tcgcatcctc atcctcgaag acacgaacgg cgaccaccgc gcggacaaat gcagcgtgtg
     1741 ggccgacaag ctcgccatcc cgctcagctt cgagttcggc aatggcggcg tcttcgtctc
     1801 cgaccagccg catctgacct tcctcaaaga caccgatggc gatggcaagg ccgaccaccg
     1861 cgaaaaactc ctcaccggct tcggcaccga ggacagccac cacgcgctgc acgacttcgt
     1921 ctggagcccc gagggcgatc tcatcttccg cgagagcatc ttccatcaca gccaggtcga
     1981 gacgccctac ggccccgtcc gcgcccgcga gagcagcttc ttccgctaca cgcccgccac
     2041 cggaaaactg ctcgcctttg gcagctacat gagcacgaac ccctggggca tcaccttcga
     2101 cgactggggt ttccacgtcg gcagccatcc cgtcttcgct agcgccgtgc acgcgctcaa
     2161 tgcgccgtat cccgacatcc acatccctgc cgggaactac tttcctgctt acagcggcac
     2221 ctgcggccag gaattcctca ccagcagcca ttggcccaaa gacctgcgcg acaaaaaaca
     2281 cttcatgcgc gtgcgctaca agcccaccaa cgaagtcgag ctgcacgagt gggtggagca
     2341 cgacacgcac ttcgaggaaa agaaagtcgg catcgtcttc cagagcacca atttgagctt
     2401 catccccgtg gacatccagc aaggccccga cggcgcgatg tacatcgccg actggtacaa
     2461 ccccgtcaaa ggccacatgc aatactccct gcgcgacacc cgccgcgaca aaaaatccgg
     2521 ccgcatctgg cgcatcaccg ccaaagacca gccgctggag gaagcgccaa aaatcgccgg
     2581 tgcgagtgtg cccgagctgc tggagctgct gaagagcgag aactaccgga cgcggtatcg
     2641 cgcgaaggtg gagttgaggg agagggaacc ggaagaaatt actgttggca ttggtttaag
     2701 cggcaggttt caccttaaca ctgaccaagc tctggctgag tggttgtggc tcaacgactg
     2761 gcttgtaaaa caaaatttcg aaaatggcat aacgccagaa atatcaaagg ctgaaacctg
     2821 ggatttcagc aatcacatgc tttcaagaat catgcgcgaa catgacaagt tcaaccatct
     2881 catgcgggcc gtggcagcgc gtagtctgcg aaagttgccc ctacgtgagt ggaaagagat
     2941 cgtgggaccg caaccccttc gaattgcagc caacgacccc agcggcctcg tccgcctcga
     3001 agccgccatc gcggcgagtt acatcggcac gccggaggcc ttggaggccg cgctcgatct
     3061 gctgaagcac ccgatggacc cttacctcac ctacgccctg cgcacctcgc tcgacagcca
     3121 cacgctgcag ccgctctgga agggcaacac cgccttcatg gcgaagcatc ccgagttggt
     3181 aaagttcctc gaagacagcg agccgaagca gcccgcgcgc atggcgaaaa agaagaagcc
     3241 tgaagcgccc catccctttg acgcgcagcc cggcctgcaa accgtcacca tcaagacgat
     3301 ccccgagcgc ctgctttatg acgtgcgcga tttcaaggtg aaggccggcc agccggtgaa
     3361 gctcgtcttt gaaaacccgg acgtgacccc gcacaacctc ctcatcgtcc agcccggcgc
     3421 ggcggacgag gtgggcatgg cggggaatga aatggccaag ctgcctgacg gcatggcgaa
     3481 gggctttctg ccggacagcc cgaagatcct gcatcacacc aggatgctga tgcagaacga
     3541 gagcgagacc ctgcgcttca ccgcgccaga aatggccggc gcctatccct acatctgcac
     3601 cttccccggc cactggctcg tcatgaaggg cgagatggtc gtcgagtgag gcggcttgcg
     3661 cccgcgcgca tccggcgcaa aaggggtggg catgtccctt ttcccctccg aagccgcccg
     3721 gctcgaagcc ttccccgtcg cgcgtgacag catcttcctc gctcatgccg gcgtcaccat
     3781 cctgccgcgc gtcgtggcgc gcacgatgca ggactacctg gagcaatgca gcctgctcat
     3841 gcaggagtat cccgaggctt ggcgcgccgt gaatgagacc cgcgtcaccg ccgcgcggct
     3901 catcggggcg aaggcgcgcg agatttccct gctcggcccc acctccgtcg gcctcagcct
     3961 cgtcgccaac ggcctcgact ggcagccggg cgacgaggtc gtctgctact ccgatgacta
     4021 ccccgccaac gtctatccct ggaccggcct cgcgcgccgc ggcgtcaccg tccgcctgct
     4081 gcggcctgag gag
//
LOCUS       DAOMYJ010000334         4074 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Prosthecobacter sp. isolate AMR_MDS_5629
            NODE_7796_length_4074_cov_19.706892, whole genome shotgun sequence.
ACCESSION   DAOMYJ010000334 DAOMYJ010000000
VERSION     DAOMYJ010000334.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867850
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prosthecobacter sp. (wastewater metagenome)
  ORGANISM  Prosthecobacter sp.
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Verrucomicrobiaceae; Prosthecobacter.
REFERENCE   1  (bases 1 to 4074)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4074)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.30
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:34:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,742
            CDSs (total)                      :: 4,684
            Genes (coding)                    :: 4,676
            CDSs (with protein)               :: 4,676
            Genes (RNA)                       :: 58
            tRNAs                             :: 55
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4074
                     /organism="Prosthecobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7796_length_4074_cov_19.706892"
                     /isolate="AMR_MDS_5629"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1965333"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(148..1386)
                     /locus_tag="PLP58_21215"
     CDS             complement(148..1386)
                     /locus_tag="PLP58_21215"
                     /inference="COORDINATES: protein motif:HMM:NF018743.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1343 domain-containing protein"
                     /protein_id="HRJ11115.1"
                     /translation="MTTRRSFLLASGAAALGGCQFAPPMPPHGQPEGGPFMLGIDFLA
                     ARNFDLLRGRRVGLITNHTSMTGRGEMTRVVMQRALGPALTALYAPEHGIDGTVGAGV
                     HVSTRRDAVTGLTVHSLYGPTRKPTPQMLAPVDTLVFDLQDIGCRSYTYISTMAVAME
                     AATECGKHFVVLDRPNPMGGWRVEGPPLEARWKSFVGQVPVPYVHGMTAGELALMAGA
                     RGWVAATPRLNVVRMQGWNRGMVWQDTGLRWHRTSPNIPHAVSPFYYVATGMLGGAAA
                     VDIGIGTENPFGYAGAAGVNPQAMLAACRGFGAAGVGFEPYARNGFGGVRLSIHPRAQ
                     ADLTALDVRMLDALNRLSGGRVLARMSGAKLNLFHKVYGSERLYNDLRRGVPAAQITA
                     GWSASVRAFMSARQRHLLYA"
     gene            complement(1417..1782)
                     /gene="queD"
                     /locus_tag="PLP58_21220"
     CDS             complement(1417..1782)
                     /gene="queD"
                     /locus_tag="PLP58_21220"
                     /EC_number="4.1.2.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009960776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-carboxytetrahydropterin synthase QueD"
                     /protein_id="HRJ11116.1"
                     /translation="MRCRIIKDFRFEAAQTLPTLPSSHKCTRMHGHSFKVEVTVEGVV
                     DPAIGWVYDHAEISGAMKPLLELLDHSYLNDIEGLENPTIENMAGWLWRKLAPQLPGL
                     AEITIHETPSARCVFRGEF"
     gene            complement(2032..2994)
                     /locus_tag="PLP58_21225"
     CDS             complement(2032..2994)
                     /locus_tag="PLP58_21225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HRJ11117.1"
                     /translation="MPQDRLAKALEQLKTISPEDHAKVKGLAAIVQPLRSFIHKTPDD
                     YGMTGWHDLVIPSDDGTPLEGWFIPAKGGESDKLVIFNHALPMCRSGFPGHFGPPWSN
                     FDAVEIDFVIQYKHLTEAGYNVLTYDIRNHGNSAAANGGLSGIGQWEWRDCVGVKKYV
                     DAHPRLSKMKVGLYSQCMGGNSQYHAIHRRPELFANVRCMVSPMVVSMAAIYDAFSEL
                     QGIRQYQELIDLELMKMGGFVAAEMTPHHWAASVTMPVLMVQVLEDEWTRNPEDAQKT
                     FDLLGSADKELFWIENTKKRFKDGYNHFGRHPDKVLSFLAKHMA"
     gene            complement(3147..3668)
                     /locus_tag="PLP58_21230"
     CDS             complement(3147..3668)
                     /locus_tag="PLP58_21230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ11118.1"
                     /translation="MFAEALTCFESAVSSTQFHGYGPRPIPHDLDGAVDRFIDAYLSA
                     THEERRLLECLPTNAASVLLAFAERQATLAVRVKSKELLVRALIAVGLSAEIRADERE
                     GMMILPLPWHSAQFLDYNPADVFKQAATVLPAVGAQALVSFSQRSPDDQTLDCMGYRT
                     GSDEGGFRYVRTW"
BASE COUNT          731 a         1334 c         1276 g          733 t
ORIGIN      
        1 cacatggatg cgccgttcgc gccccgcgct ctgcgcacgc ggcgcttgga ggaccaagcg
       61 ccctaccccg cccccgcagg cgcattcgcc agaatgcggg gctcaggtgc cgcggcggca
      121 gtcaaacccg cgctatggca acagcgccta cgcgtagagc agatgccgct ggcgcgcgct
      181 catgaaagcc cgcacgctgg cggaccaccc cgccgtgatc tgcgccgccg gcaccccgcg
      241 ccgcaggtcg ttgtaaaggc gctcgctgcc atagaccttg tggaagaggt tcagcttcgc
      301 cccgctcatg cgcgccagca ccctgccgcc ggaaaggcgg ttcagcgcgt ccagcatgcg
      361 cacatccagc gccgtcagat ccgcctgcgc ccgcggatgg atggagagcc gcaccccgcc
      421 gaagccgttg cgcgcatacg gctcgaagcc cacccccgcc gcgccgaagc cccggcacgc
      481 cgccagcatc gcctgcgggt tcacgcccgc ggcccccgcg tagccaaaag gattctccgt
      541 gccgatgccg atgtccaccg ccgccgcgcc gcccagcatg cccgtcgcca cataatagaa
      601 aggcgacacc gcgtgcggga tgttcggcga ggtgcggtgc cagcgcagcc ccgtgtcctg
      661 ccacaccatg ccgcggttcc agccctgcat ccgcaccaca ttcaggcgcg gcgtggcggc
      721 cacccagccg cgcgcccccg ccatcagcgc cagctccccc gccgtcatcc catgcacata
      781 cggcaccggc acctggccca cgaaggactt ccagcgcgcc tccagcggcg gcccctccac
      841 gcgccacccg cccatcggat tcggcctgtc cagcaccaca aaatgcttcc cgcactccgt
      901 cgccgcctcc atcgccaccg ccatcgtgct gatgtaggtg tagctgcggc agccgatgtc
      961 ctgcagatcg aacaccagcg tgtccaccgg cgccagcatc tgcggcgtcg gcttccgcgt
     1021 cggcccgtag agggagtgga ccgtcagccc cgtcaccgcg tcccgccgcg tgctcacgtg
     1081 gacgcccgcc cccaccgtcc cgtcaatccc gtgctccggc gcgtacagcg ccgtcagcgc
     1141 cggccccagc gcccgctgca tcaccacccg cgtcatctcc ccgcgccccg tcatgctcgt
     1201 gtggttggtg atcagcccca cgcgcctgcc gcgcagcagg tcaaagttcc gcgccgccag
     1261 aaaatcaatc cccagcatga aagggccgcc ctcgggctgc ccatgcggcg gcatcggcgg
     1321 ggcgaactgg cagccgccga gcgcggcggc acccgaggcg agcaggaagg agcggcgcgt
     1381 ggtcatggca tccggcgggc aaagcggcgc gtgaaattaa aactcccccc ggaacacgca
     1441 gcgcgccgag ggcgtctcat ggatcgtgat ctccgccagc cccggcaatt gcggcgccag
     1501 cttccgccac agccaccccg ccatgttctc gatcgtggga ttctccaggc cctcgatgtc
     1561 gttgaggtag gaatggtcca gcaactccag cagcggcttc atcgccccgg agatctccgc
     1621 gtggtcgtac acccacccga tcgccgggtc caccacccct tccaccgtga cctccacctt
     1681 gaagctgtgt ccgtgcatgc gcgtgcattt gtggctcgag ggcagcgtcg gcagcgtctg
     1741 ggcggcctcg aagcggaaat ctttgatgat gcggcagcgc atgggcggaa aaagcgggaa
     1801 ctcacaggca tctcccgctc cccgcccggc tcgtcaaggc gcaatttcac ccgcctgcgc
     1861 gggagcgccg ccttgtctgg agcgcggtac tcgcgcaatc atggtttagc cgaacgaccc
     1921 aagctcagcg acggcggagc gtggcgcggt agctgcgaag gggagtgcag aaagcggcca
     1981 cggacgtagg acagaggtgg ctcggacgag acaggttagg ccacattggt gctacgccat
     2041 gtgcttggcc aaaaacgaca gcaccttgtc cgggtgtctt ccaaagtggt tgtatccgtc
     2101 cttgaaacgc ttcttggtgt tctcgatcca gaacagctct ttgtctgcgc tgcccagcag
     2161 gtcgaaggtc ttttgtgcgt cttcgggatt gcgggtccat tcgtcttcca gcacctgcac
     2221 catcagcacc ggcatggtga cgctcgcggc ccagtgatgc ggagtcatct cggcggcgac
     2281 gaagccgccc atcttcatca attcgaggtc aatgagttcc tgatactgcc ggatgccttg
     2341 tagctcggag aaggcatcgt aaatcgcggc catcgagacg accatcgggc tgaccatgca
     2401 gcgcacgttg gcgaagagtt cgggtcggcg gtggatggcg tggtattgcg agttgccgcc
     2461 catgcactgg ctgtagagcc cgaccttcat cttcgagagg cgcgggtggg catccacgta
     2521 tttcttcacg cccacgcaat cgcgccactc ccactggccg atgccgctca agccgccgtt
     2581 cgccgcggcg ctgttgccgt ggttgcggat gtcgtaggtg agcacgttat agccggcctc
     2641 ggtgaggtgt ttgtattgga tgacaaaatc aatctcgacc gcgtcgaagt tgctccacgg
     2701 cgggccgaag tgtccgggaa aaccggagcg gcacatgggc agggcgtgat tgaagatgac
     2761 gagcttgtcg ctctcgccac ccttggccgg gataaaccaa ccttcgagcg gcgtgccatc
     2821 gtcggaagga ataaccaagt cgtgccagcc cgtcatgccg tagtcatccg gcgtcttgtg
     2881 gatgaagctt cgcaagggct gcacgatggc ggcgagacct ttcaccttgg cgtggtcctc
     2941 cggcgaaatg gtcttgagtt gttcgagagc cttggcgagg cggtcttgtg gcattcggct
     3001 catagtattt gtgtgtggtg gattgtgttg gcggattcgc tgcggcctaa cgacccatac
     3061 agcgacccgg cccgcgggac gccacgatca caaccgcgac gctcatgccg ggttcgctgc
     3121 agcgcatggc aggcccgttt tgctctctac caagtgcgga catagcggaa ccctccttca
     3181 tctgaaccgg ttctgtatcc catgcaatca agtgtctgat cgtcaggtga acgctgagaa
     3241 aagctgacaa gcgcttgtgc gccgacggcg ggaagaacgg tcgccgcctg cttgaaaacg
     3301 tcggcgggat tgtagtcaag gaactgcgcc gaatgccagg gcagcggcag aatcatcatg
     3361 ccttcgcgtt cgtctgcacg aatttcggcg gaaagcccca cggctataag agcgcggaca
     3421 agaagctctt tcgacttcac acggaccgcg agcgtagcct gacgttctgc gaaggcgagc
     3481 agcacgctgg cagcgttggt cggtagacat tccagtagtc ggcgttcctc atgggttgcg
     3541 gagaggtagg cgtcgatgaa ccgatcgact gccccgtcaa gatcatgcgg aatcggccgc
     3601 gggccgtatc cgtgaaattg cgtgctcgat acagcagatt caaagcaagt taatgcttcg
     3661 gcgaacattg atggtgcggt tgcgatgggc ctaacttatt gatgagacac aaatttgtca
     3721 attacgtcat ccccattcta gccggcaaat gggtggtttt ttcacggttg gggaatgtgc
     3781 atgaccggtt ttaattcgaa cgcgggtcat gagcaacaag gcagcggatt atggcgcaga
     3841 tgcgtgtcat caaaccggtg aaatgatcac cgggtgaaac ttaaaagctg ccagacatct
     3901 gcgttcactg gcacgattgc ggagtcgtgc ggcaggtggg gaggctgcct tggcacgttt
     3961 gcggagtcgt gcggcaggtg gggaggctcc cttggcacgt ttgcgaagtc gcgcggcagg
     4021 tggggaggct ctttttgggg cggaaaaggc tttggggctg cctccagggc tgac
//
LOCUS       DAOMYJ010000335         4068 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Prosthecobacter sp. isolate AMR_MDS_5629
            NODE_7817_length_4068_cov_21.437578, whole genome shotgun sequence.
ACCESSION   DAOMYJ010000335 DAOMYJ010000000
VERSION     DAOMYJ010000335.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867850
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prosthecobacter sp. (wastewater metagenome)
  ORGANISM  Prosthecobacter sp.
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Verrucomicrobiaceae; Prosthecobacter.
REFERENCE   1  (bases 1 to 4068)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4068)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.30
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:34:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,742
            CDSs (total)                      :: 4,684
            Genes (coding)                    :: 4,676
            CDSs (with protein)               :: 4,676
            Genes (RNA)                       :: 58
            tRNAs                             :: 55
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4068
                     /organism="Prosthecobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7817_length_4068_cov_21.437578"
                     /isolate="AMR_MDS_5629"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1965333"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..744
                     /locus_tag="PLP58_21235"
     CDS             <1..744
                     /locus_tag="PLP58_21235"
                     /inference="COORDINATES: protein motif:HMM:NF014625.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L family deacetylase"
                     /protein_id="HRJ11119.1"
                     /translation="GHHVKLVSVTNGDIGHWREAGGPLALRRAAEAAACAKKLGVASQ
                     VLDIHDGELMPTLENRKLITRVIREWQADIVIAHRPWDYHPDHRYVGVLIQDAAFMVT
                     VPFICPDVPPLKKNPVFLYSSDGFQKPYPFTPDIAVSVDDSFDQKLDGLHELTSQAYE
                     GGANGSAEHVEKQVPPASDETARRAWLKRRWGARQGSEADKFRELLVKWHGPEKGAAV
                     RYAETFEVCEYGRRPSEDELRKLFPFFGN"
     gene            838..3594
                     /locus_tag="PLP58_21240"
     CDS             838..3594
                     /locus_tag="PLP58_21240"
                     /inference="COORDINATES: protein motif:HMM:NF012905.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HRJ11120.1"
                     /translation="MRLFHAAAMNNDASPAAPAYWHHLEMREVCRLLRADLQHGLSVD
                     EVRRRRKEYGPNSVTARGGTPAWKIFLMQFNQPLVCILLVAVAITAFLREWVDSGVIF
                     GVVFINAVIGFIQESKAGKAIEALSRMVRTEATVRRGGKRLRVPSEELVPGDVVLLQS
                     GDRVPADLRLFDVHSLQVDESALTGESLPAGKHPDPLDHDVVLADRRNLAYAGTLVAA
                     GQAEGMVWAIGDKTETGRIAWLIHEAVEISTPLTKKITEFSKLLLWVILALAALTFFI
                     GLARGQPAVDTFMAAVALAVGAIPEGLPAAVTIVLAIGVSRMARRRAIIRKLPAVETL
                     GSTTVICSDKTGTLTENQMTVQRVFAGGEWFDVTGGGYDTRGDIRQAGAKLDVESKPA
                     LRECLLAGVLCNESQLLTDDDGRAGMQGDPTEAALLVAGAKAGLIHEETHAGTPRLDM
                     IPFDSAHMFRATLHQAGGDTMIYKVGAVERLLERCTDALGPDGSLMPLDKESVRRAVE
                     EMAAGGLRVLAFTRRHGGHVEGALGHEHVSEGLTFLGLQGMMDPPRPEAIQAVAKCRQ
                     AGIKVKMITGDHAVTARAIATRIGLADEDGVQAVTGRELEDVPESELPALAEKTEVFA
                     RVAPDQKLRLVQALQACGHVVAMTGDGVNDAPALKQADIGIAMGIAGTDVAKGAADMI
                     LTDDNFASIKAAVEEGRGVFDNLRKFIIWTLPTNLGEGAIILVAILLGVTLPVLPVQL
                     LWVNMATAIFLGLMLVFEPKEPDLMLRRPRDPRRPLLTFPLIMRTGLVSLFIIGGGFW
                     LFFHEMNVLGETESEARTAVVNVIVMVETAYLFSCRSLNHSVFSIGLLTNPLALAGAS
                     AMIAAQVLFTYLPLMNRLFHTTPLNAWSWVPIGAVALLSFVAVELEKWIRFGGGRGGN
                     AELD"
     gene            3688..>4068
                     /locus_tag="PLP58_21245"
     CDS             3688..>4068
                     /locus_tag="PLP58_21245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ11121.1"
                     /translation="MSLRETIFTLAIASAVVLGVVVVGLIARGPRKEPVQAIPVKVAG
                     IPGQTLPDEGGRPKEVSEQFQIFPRPELVESRANEADTLRVRSGQEEHIFVLYFVDAL
                     ETTENHPQRVAEQARYFMVKDVETI"
BASE COUNT          693 a         1314 c         1340 g          721 t
ORIGIN      
        1 ggtcatcatg tgaaactggt gtccgtgacc aatggcgaca tcggccactg gcgagaggcg
       61 ggcgggccgc tggcgttgcg ccgcgccgcc gaggccgccg cctgcgcgaa aaaactgggc
      121 gtcgcctccc aggtgctcga catccatgac ggcgagctca tgcccacgct ggagaaccgc
      181 aagctcatca cccgcgtcat tcgcgagtgg caggccgata ttgtcatcgc ccaccgcccg
      241 tgggactacc atcccgacca tcgttatgtg ggcgtgctca tccaggacgc cgccttcatg
      301 gtcacggtgc ccttcatctg cccggacgtg ccgccgctga aaaagaaccc ggtgttcctg
      361 tattcgagtg acggttttca aaaaccctac cccttcacac cggacatcgc cgtcagcgtg
      421 gacgactcct ttgaccagaa actcgacggc ctgcacgagc tgacctccca ggcctatgaa
      481 ggcggcgcca acggctccgc tgagcacgtc gaaaaacaag tgccccccgc ttccgacgag
      541 accgcccgcc gcgcctggct caagcgccgc tggggcgcgc gccagggcag cgaggcggac
      601 aagttccgcg aactgctcgt caaatggcac ggccctgaga agggtgcggc ggtgagatac
      661 gctgagactt tcgaggtctg cgaatatggc cgacgcccct ccgaggacga gctgcgcaag
      721 ttattcccat tcttcggcaa ctgagtcact tcccggcctt ggcctggacc tctccgatct
      781 tgggaaatga cgccccgatt ctggaggcgc ggaccgcctt ttcttgcaat cgcggggatg
      841 cgccttttcc atgccgccgc catgaacaat gacgccagtc ccgccgctcc tgcttattgg
      901 caccacctcg aaatgcgcga ggtgtgccgc ctgctccgcg ccgatctcca gcacgggctc
      961 agcgtggacg aggtgcggcg caggcggaag gaatacggtc cgaacagtgt caccgcgcgc
     1021 ggcggcacgc cggcgtggaa gatcttcctc atgcagttca accagcctct ggtgtgcatt
     1081 ctgctcgtcg cggtggcgat cacagctttc ctccgggagt gggtggattc cggggtcatc
     1141 tttggcgtgg tcttcatcaa tgccgtcatc ggcttcatcc aggagtccaa ggcgggaaaa
     1201 gccatcgagg cgctgagccg catggtgcgc accgaggcca cggtgcggcg cggcggcaaa
     1261 aggctgcgcg tcccctcgga ggagctggtg ccgggggatg tcgtgctgct gcagtccggc
     1321 gaccgcgtgc ctgcggactt gcggcttttc gatgtccaca gcctccaggt ggacgagtcc
     1381 gcgctcacgg gagagtcgct gcctgccggc aagcaccccg atcccctgga tcatgacgtg
     1441 gtgctggccg accggagaaa cctggcctac gcgggcacgc tcgtggctgc aggccaggct
     1501 gagggcatgg tgtgggccat tggagacaag acggagacgg gccgcatcgc ctggctcatc
     1561 cacgaggcgg tggagatttc cacaccgctg acgaaaaaaa tcaccgagtt cagcaagctg
     1621 ctgctctggg tgatcctggc gctggcggct ctgacctttt tcatcggcct cgcgcgcggc
     1681 cagcccgcgg tggacacctt catggcggcg gtggcgctgg ccgtgggcgc gatcccggag
     1741 gggctgccgg cggcggtgac catcgtgctt gccatcggtg tcagccgcat ggcgcggcgg
     1801 cgcgccatca tccgcaagct gcccgccgtg gagacgctgg gcagcaccac ggtcatctgc
     1861 tcggacaaga ccggcactct caccgagaac cagatgactg tgcagcgcgt ctttgccgga
     1921 ggcgagtggt tcgatgtcac cggcggaggc tatgacacgc gcggggacat ccggcaagcc
     1981 ggcgcgaaat tggatgtcga aagcaaaccc gccctgcgcg agtgcctgct ggctggcgtg
     2041 ctctgcaatg aatcccaact gcttaccgat gacgacggac gggccgggat gcaaggcgac
     2101 cccaccgagg ccgcgctgct cgtggccggg gcaaaggcgg gtctcattca cgaggaaacc
     2161 cacgcgggca cgccaaggct ggacatgatc cccttcgact ccgcgcacat gtttcgcgcc
     2221 acgcttcacc aggcgggcgg ggacacgatg atctacaaag tcggcgccgt cgagcgcctg
     2281 ctcgaacgct gcacggacgc gcttggtcct gatggcagcc tcatgcctct ggacaaggaa
     2341 tccgtgcgcc gcgcggtgga ggagatggcc gccggcggcc tgcgcgtgct ggccttcacc
     2401 cgccggcatg gcgggcatgt cgagggcgcg ctcggtcacg agcatgtgag cgaggggctg
     2461 acctttctgg gactccaggg catgatggat ccgccgaggc cggaggccat ccaggccgtg
     2521 gccaaatgcc gccaggcggg catcaaagtc aaaatgatca ccggcgacca tgccgtgact
     2581 gccagggcca tcgccacgcg catcggcctc gcggacgagg acggcgtgca ggctgtgacc
     2641 gggcgtgaac tggaggacgt tccggagagc gagctgcccg cgctggccga gaagaccgag
     2701 gttttcgcgc gcgtcgcgcc cgatcaaaaa ctgcgccttg tgcaggccct gcaggcgtgc
     2761 ggccatgtgg tggccatgac gggcgacggc gtcaatgacg cgcccgcctt gaagcaggcc
     2821 gacatcggca ttgccatggg catcgcgggc acggatgtgg cgaaaggcgc ggcggacatg
     2881 atcctcacgg atgacaactt cgccagcatc aaggccgccg tggaggaggg gcgcggtgtc
     2941 tttgacaacc tgcgcaaatt catcatctgg accctgccaa ccaatctcgg cgagggcgcc
     3001 atcatcctcg tggccatcct gctcggcgtc acgctgccgg tgctgcctgt gcagctcctg
     3061 tgggtgaaca tggccaccgc catctttctc ggcctgatgc tcgtctttga gccgaaggag
     3121 cctgacctca tgctgcgccg cccgcgtgat ccgcggcggc cgctgctgac cttcccgctc
     3181 atcatgcgca cggggctggt ctcgctgttc atcatcggcg gcggcttctg gctgtttttc
     3241 cacgagatga atgtgctcgg cgagacggag tccgaggcgc gcacggcggt ggtgaatgtc
     3301 atcgtcatgg tcgagacggc ctatctcttc agttgccgct cgctgaacca ctcggtgttc
     3361 agcatcggcc tcctcaccaa tccgctcgcg ctcgcgggag cgtcggccat gatcgccgcg
     3421 caggtgctct tcacctacct gccgctgatg aaccgccttt ttcacaccac gccgttgaac
     3481 gcctggtcct gggtgccgat cggcgcggtg gccctgcttt cattcgtcgc tgtggagttg
     3541 gagaagtgga tccgctttgg cggcgggcgc ggaggcaatg cggagctgga ctgaccggaa
     3601 tcccggtggt gaaaaatggc ccggctgtcg ttttcccgtg ttgaacagct tgagaaacac
     3661 gggctttgca gggtatgttc ccggctcatg tctctccggg aaacgatctt caccctcgcc
     3721 atcgcctccg ccgtggtgct cggagtggtg gtcgtcggcc tcatcgcgcg aggaccccgc
     3781 aaggaaccgg tgcaggccat tcccgtgaaa gtcgccggca ttccgggaca gactctgccc
     3841 gacgagggcg gacgaccgaa ggaggtcagc gagcagtttc aaatctttcc ccgccccgaa
     3901 ctggtcgaaa gccgggcgaa tgaggctgac accctgcgcg tgcgcagcgg gcaggaggag
     3961 cacatttttg ttctctactt tgtggacgcc cttgaaacga cggagaacca tccccagcgc
     4021 gtggcggagc aggcccgcta tttcatggtg aaggatgtcg aaaccatc
//
LOCUS       DAOMYJ010000336         4067 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Prosthecobacter sp. isolate AMR_MDS_5629
            NODE_7821_length_4067_cov_20.985045, whole genome shotgun sequence.
ACCESSION   DAOMYJ010000336 DAOMYJ010000000
VERSION     DAOMYJ010000336.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867850
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prosthecobacter sp. (wastewater metagenome)
  ORGANISM  Prosthecobacter sp.
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Verrucomicrobiaceae; Prosthecobacter.
REFERENCE   1  (bases 1 to 4067)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4067)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.30
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:34:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,742
            CDSs (total)                      :: 4,684
            Genes (coding)                    :: 4,676
            CDSs (with protein)               :: 4,676
            Genes (RNA)                       :: 58
            tRNAs                             :: 55
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4067
                     /organism="Prosthecobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7821_length_4067_cov_20.985045"
                     /isolate="AMR_MDS_5629"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1965333"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            15..1031
                     /gene="floA"
                     /locus_tag="PLP58_21250"
     CDS             15..1031
                     /gene="floA"
                     /locus_tag="PLP58_21250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012419766.1"
                     /note="flotillin-like protein involved in membrane lipid
                     rafts; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flotillin-like protein FloA"
                     /protein_id="HRJ11122.1"
                     /translation="MNFQFLLVGGAIIVALVLFLIIAKFFNLWLRARISNAPVSIINL
                     LAMYLRGIPNSMVVDSRITAVKAGIPLETDPLEAHYLAGGDVQHVVLALVAADKAGIP
                     LTFDRACAIDLACKGTSKTVLDAVRTSINPKVIDCPNQEMGRGGKIDAVAKDGISLRV
                     RARVTVRTNLDRFVGGATEETVIARVGEGIVSSIGSAGSYKEVMENPDSISKLVLAKG
                     VDAGTAFEILSIDIADIDVGENVGAKLQTEQAEADKKIAQANAEMRRAAAVALEQEMS
                     ARTQEMRARVVEAEAQVPLAMAEAFRAGNLGVMDYARFRNIAADTDMRAKIAGGEGGG
                     TQQE"
     gene            1197..2048
                     /gene="purU"
                     /locus_tag="PLP58_21255"
     CDS             1197..2048
                     /gene="purU"
                     /locus_tag="PLP58_21255"
                     /EC_number="3.5.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009961359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008864 - formyltetrahydrofolate
                     deformylase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formyltetrahydrofolate deformylase"
                     /protein_id="HRJ11123.1"
                     /translation="MPTATLLIHCPDKPGLVHDVTGFILSHQGNIIDLQQHIDPEQGA
                     FFMRLEWSLERFTLEPEEIESRLQPLARQHQMRLTLSLSSRRKRVALLVSRDSHCLYD
                     LLARHEAGDLPVDIPLILSNHEHLRPAAERFGIPFHHFPVTPQTKDAQEQAQISLLRA
                     HDVDTLVLARYMQILSPRMIEAFPLRILNIHHSFLPAFVGARPYHQAHERGVKIIGAT
                     SHYVTAELDQGPIIHQDVMRVSHEHSVADLVRLGRDLEKTVLAKALWWHVRDRVLVFQ
                     NKTLVFE"
     gene            complement(2090..2743)
                     /locus_tag="PLP58_21260"
     CDS             complement(2090..2743)
                     /locus_tag="PLP58_21260"
                     /inference="COORDINATES: protein motif:HMM:NF014244.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HRJ11124.1"
                     /translation="MLRFRLFGFPILVHWFFWVNMALLGGAIGADTPEAMQRLLMWMA
                     AGFVSIVVHELGHAFAMRHYGDRHVNILLYAFGGLAQGSRGLTRRQDFFVSGAGPFAQ
                     IAAGVVMWWVYDLLGPGPGLPGYFMRAFMMVSFFWAVLNLFPIIPLDGGHICLAVLGP
                     RRVRLALVISLVCAVAGGLWFLSARSIFAALFFGMFAFNNWKELQGRPQTPWMMGRN"
     gene            complement(2915..3346)
                     /locus_tag="PLP58_21265"
     CDS             complement(2915..3346)
                     /locus_tag="PLP58_21265"
                     /inference="COORDINATES: protein motif:HMM:NF025484.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4288 domain-containing protein"
                     /protein_id="HRJ11125.1"
                     /translation="MKRSKPWYAATLIVEVRVEGRKTCDAHLNTVLIQAASPEDAHAK
                     ALVLGKEADADFINLQGRKAVLRFRGLRDLTFVCDELEHGAEILWRRKARLNQAGIRR
                     LMTRKSELGVFKKAGPLIAEIMPADTAEVLLNRAGKSRPGR"
     gene            complement(3509..3844)
                     /locus_tag="PLP58_21270"
     CDS             complement(3509..3844)
                     /locus_tag="PLP58_21270"
                     /inference="COORDINATES: protein motif:HMM:NF016167.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF433 domain-containing protein"
                     /protein_id="HRJ11126.1"
                     /translation="MTATFDSIRDAVLHLPKGDQVRILALVALDVADAHPGIDFQESV
                     CGGAARIIRTRIPVWLLESLRRGGKTDAELLAAYPSLNAEDLANAWNYARTHREEMDR
                     EIAANGDES"
BASE COUNT          772 a         1548 c         1108 g          639 t
ORIGIN      
        1 acccccgcac ccgcatgaac ttccagttcc tcctcgtcgg cggcgccatc attgtcgccc
       61 tggtcctctt cctcatcatc gccaagttct tcaacctctg gctgcgcgcc cgcatctcca
      121 acgcgccggt gagcatcatc aacctcctgg ccatgtacct gcgcggcatc ccgaactcca
      181 tggtcgtgga ctcccgcatc accgccgtca aagccggcat ccccctggag accgaccccc
      241 tcgaagccca ctacctggcc ggcggcgatg tccagcacgt cgtgctcgcc ctcgtcgccg
      301 ccgacaaggc cggcatcccc ctcacctttg accgcgcctg cgccattgac ctcgcctgca
      361 aaggcaccag caagaccgtg ctcgacgccg tccgcaccag catcaacccc aaggtcattg
      421 actgccccaa ccaggaaatg ggacgcggcg gcaaaattga cgccgtcgcc aaggacggca
      481 tctccctgcg cgtccgcgcc cgcgtcaccg tgcgcaccaa tctcgaccgc ttcgtcggcg
      541 gcgccaccga ggagaccgtc atcgcccgtg tcggcgaggg catcgtctcc tccatcggct
      601 ccgccggctc ctacaaggag gtcatggaaa accccgacag catctccaag ctcgtcctgg
      661 ccaaaggcgt ggacgccggc accgccttcg agatcctctc catagacatc gccgacattg
      721 acgtcggcga aaacgtcggc gccaagctcc agaccgagca ggccgaggcc gacaaaaaaa
      781 tcgcccaggc caacgccgaa atgcgccgcg ccgccgccgt cgccctcgag caggaaatgt
      841 ccgcccgcac ccaggaaatg cgcgcccgcg tcgtcgaagc cgaagcccag gtccccctcg
      901 ccatggccga ggccttccgc gccggcaacc tcggcgtcat ggactacgcc cgcttccgca
      961 acatcgccgc cgacaccgac atgcgcgcca agatcgccgg cggcgagggc ggcggcaccc
     1021 agcaggagtg atcccgccgc ggcggcctgg catggttcca accagcgccg ccatgatttg
     1081 tgcttccccg ccgcctgcag ttctggcaac cgtctggtgg agtcctgcgg cggccggccg
     1141 gctcgatacc gccagcactc catgactcca cccctccgac tccgcaagcc cagcccatgc
     1201 ccaccgccac cctgctcatc cactgcccgg acaagcccgg cctcgtgcat gacgtcaccg
     1261 gcttcatcct ctcccatcag gggaacatca tagacctcca gcagcacata gaccccgagc
     1321 agggcgcctt cttcatgcgc ctggagtgga gcctggagcg cttcaccctc gaaccggagg
     1381 agatcgagag ccgcctccag cccctggcgc gccagcatca gatgcgcctc accctcagcc
     1441 tctcctcccg gcgcaagcgc gtggccctcc tcgtcagccg ggacagccac tgcctctacg
     1501 atctcctcgc ccgccacgag gccggcgacc tgcctgtgga catccccctc atcctctcca
     1561 accacgagca cctccgcccc gccgccgagc gtttcggcat ccccttccac cacttccccg
     1621 tcacacccca gaccaaggac gcccaggagc aggcccagat cagcctcctg cgcgcgcacg
     1681 atgtggacac cctcgtcctg gcccgctaca tgcagatcct cagcccccgc atgatcgagg
     1741 ccttccccct ccgcatcctc aacatccacc acagcttcct ccccgccttc gtcggcgcca
     1801 ggccctacca ccaggcccac gagcgcggcg tcaagatcat cggcgccacc agccactacg
     1861 tcaccgccga gctggaccag ggacccatca tccaccagga tgtcatgcgc gttagccacg
     1921 agcacagcgt cgccgatctc gtccgcctcg ggcgcgacct ggaaaaaacc gtcctcgcca
     1981 aagccctctg gtggcatgtg cgcgaccgcg tcctcgtctt ccaaaacaaa accctcgtct
     2041 tcgagtgacg ccgcgccccg cgcgcgccgg ccctccgccc gcgccgcggt cagttccggc
     2101 ccatcatcca gggcgtctgc ggccggcctt gcagctcctt ccagttgttg aaggcgaaca
     2161 tgccgaaaaa cagcgcggca aagatcgaac gcgccgagag aaaccacagc ccgcccgcca
     2221 ccgcgcacac caggctgatc accagcgcca gccgcacccg gcgcggcccc agcaccgcca
     2281 ggcagatgtg cccgccatcc agcgggatga tggggaaaag attcagcacc gcccagaaaa
     2341 agctcaccat catgaaagcc ctcatgaaat accccggcag gcccggcccc ggccccagca
     2401 ggtcataaac ccaccacatc accacccccg ccgcgatctg cgcgaagggg cccgccccgc
     2461 tcacaaaaaa atcctgccgc cgcgtcagcc cccggctccc ctgcgccagc ccgccaaaag
     2521 catagagcag gatgttcaca tgccggtccc cgtagtggcg catcgcgaag gcatggccca
     2581 gctcatgcac cacgatggac acaaagccgg ccgccatcca catcagcagc cgctgcatcg
     2641 cctccggcgt gtccgccccg atggcgccgc cgagcagcgc catgttcacc cagaaaaacc
     2701 agtgtacgag gatcggaaac ccaaaaagac ggaagcgcag catgacccgc caccttggcc
     2761 tccccatgca cgccggcaag agcgggtttc acctttcccg cacccctgaa agccgccgcg
     2821 gcagccgcca gcccaacggc ggcatccctc gacttcgaca tcccctcatc agcgtcacca
     2881 ccggcaaggc ccgcgccctt ccgggagaga gggatcatcg cccaggtctg gatttgcccg
     2941 cgcgattgag aagcacctcc gccgtatccg caggcatgat ctcagcaatg agcggtcccg
     3001 ccttcttgaa cacgccaagt tcagacttcc gcgtcatcag cctgcggatg ccggcttgat
     3061 tgaggcgcgc cttgcgcctc cacaggattt ccgccccatg ctccagttcg tcgcagacaa
     3121 acgtaaggtc gcgaagcccg cgaaaccgta ggacagcctt cctgccctga aggttgatga
     3181 agtccgcgtc ggcttccttg ccaagcacca acgccttcgc atgggcatcc tcagggcttg
     3241 ccgcttggat gagaacagtg ttgagatgcg cgtcgcaggt cttccggcct tcgacgcgga
     3301 cctccacaat caaagttgcc gcataccatg gtttggaccg tttcatgaat gccgtcgtag
     3361 ttcgtgtgtc aaattccagc gccgccgccg gagaccgtgg tgcgaatggg ttgcacatca
     3421 ttcagaacca gatggcgccc ttgtcccatg tgacgcagaa cttccacgac tggcagcgga
     3481 aagttctcgt tggaatatag ccgggcgatc atgattcgtc tccattggcg gcgatctccc
     3541 ggtccatttc ctcccgatgc gtgcgcgcat aattccaggc gttggcgagg tcctccgcat
     3601 tcagtgaagg ataggccgcc agcagttccg cgtctgtctt gccaccacgc cgcagggatt
     3661 cgaggagcca gacaggaatc cgcgtgcgga taattcttgc ggccccgccg cagacactct
     3721 cctgaaagtc gatgccagga tgcgcatccg ccacgtccag cgccacaagg gcgaggatgc
     3781 gcacttgatc gcccttgggc agatggagca cggcgtcacg gatggagtca aaggtggcag
     3841 tcatgaacgg attctatcac agagttcaga ttgggcagtc gtcttttcga tccatcaaac
     3901 actcgccccg taatgcgcgt ccagcttctc gtgcagggcg aggatgctgg cgatgtggcg
     3961 gacgtggtgg gtgaagtgtt gggcttcgtc gggggtgagg gggcggccga ggagggcgga
     4021 ctcgcggtag ctgagccact tcttgaggac ttggtagccg ccgaggg
//
LOCUS       DAOMYJ010000337         4063 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Prosthecobacter sp. isolate AMR_MDS_5629
            NODE_7830_length_4063_cov_23.206337, whole genome shotgun sequence.
ACCESSION   DAOMYJ010000337 DAOMYJ010000000
VERSION     DAOMYJ010000337.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867850
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prosthecobacter sp. (wastewater metagenome)
  ORGANISM  Prosthecobacter sp.
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Verrucomicrobiaceae; Prosthecobacter.
REFERENCE   1  (bases 1 to 4063)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4063)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.30
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:34:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,742
            CDSs (total)                      :: 4,684
            Genes (coding)                    :: 4,676
            CDSs (with protein)               :: 4,676
            Genes (RNA)                       :: 58
            tRNAs                             :: 55
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4063
                     /organism="Prosthecobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7830_length_4063_cov_23.206337"
                     /isolate="AMR_MDS_5629"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1965333"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>4063
                     /locus_tag="PLP58_21275"
     CDS             <1..>4063
                     /locus_tag="PLP58_21275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ11127.1"
                     /translation="ADDSGYFLQPLTAQASIETTPENINALWKSAVKRAADLAGQPVE
                     DWNEVARGTAANATNFIITDFLFKAGTFTTTSIIANMNEVSKAALGLEIPMARWIERD
                     ARNNPAIRSTPISIGDHAAMLLLRDEFVKLMREQHAQWQKNQAPAAMRAIFETQKDAV
                     AARRSPLSYVRVPVKQLQGYSEIEPSMLQKVSLFFREEEEPLLHTLFSREFSGGVMRV
                     DKDAPGFERLQWEAFKLAYTGWGALMQRSITDAQNLSDGDVLGLLKLTGMSFEPVVER
                     LRPKLMKLEIDAQARRQRWVPDRVARAYVTGLGVLGAAVRAQEEVSEADTRVIQATAA
                     LASVALGNVSGVWAAAAAVGISAADAAYAIYQDGYMNTARRDQLQSEIGAGAVLGADR
                     AAEAEGKILSTLEQALSIGGSAFGLTADGAVLRSLKSAKALTLAETLDKAADILKNVD
                     VADPAALRKLSKADAAVLLRAAEAAEDAAIAGRPMDLFEKIQEVVPHKDDLFEALAEG
                     TEAAGLSEKLKRVAESPAITLKRTELQDAGKNLDARIAKTEAEISDIKAKKPADGSAE
                     AKLLDAKKGELGDLKQQKTVVDAMEQPVVSVPKRTAEMLTGEATAITSAERAAVDEAS
                     KLLAGGGRFSGEGLQKVNLTSAGKRLDDLIQQSTKRLDDLRANPAAAKAGELDDAARR
                     LDDLQSARETVRKLDKAVGDASETRISQRDLDLINGARKFDDLDQAEVHKAILKSGGD
                     WKQIVSKAKKGPEEQLLLHKVAAYRRKVVHEIADEVIEGVENATGQKGLFRDAFGSAN
                     LSSDYDVAFRGPGAELAVQEFNNRVRKVLGGRESGAVLDNNFYTDPVYSIYKLGDGAG
                     AGGVTPAQLDTARQFLYQQMAGVKYRTPKQFASFRNKMLGQLPEGPRKFFDDLLDQAL
                     GAQSLAQKRIEARLAKIGKTAETATPGEMLRVTNDLYGDTLSGIHGYRSFVDHLDAVI
                     SGGGKSLRNAPVPAVLQNTEFSKQLGIIQGMIADPGTRAAGLEALKKLRDRTVLNMRN
                     QQGLALYYASEAYQAGDTIKHVVKEIQAGGHRFDPVAALKGQRPSNQGITPDGYINSY
                     YENGANLVKELNGKHVLDEATGALKSGLDPSYLEAAATKSAKYFVRQLDAANAAGADL
                     NKLTDAEQLLTATARLDAARDSPAKFNDVLKEFGWTHEEYVQRVLAANDYLDSQVVGK
                     SSLTGIAGNLVAAEKEAANLPARLQDLASQRAYQESLVRVFGDSASLAKLERQSLNAL
                     FDGQPATPPDARSVSAQKQFEASGQAARLAGLSQEEMAGIVAAVPQSGDPAAWFAGSA
                     SALEKAALDRLSSSPASPASAATSSLPPATRRAI"
BASE COUNT          849 a         1365 c         1229 g          620 t
ORIGIN      
        1 cgccgatgac agcggctatt tcctccagcc gctcactgcg caggcctcca tcgagaccac
       61 gccggagaac atcaacgcgc tatggaagtc cgccgtgaaa cgcgccgccg acctagccgg
      121 gcagccggtc gaggactgga acgaggtggc gcgcggcacc gctgccaacg ccacgaactt
      181 catcatcacc gacttccttt tcaaagcggg cacctttacg acgacctcca tcatcgcgaa
      241 catgaacgag gtttccaagg cggcgctggg cctggagatc cccatggcgc gctggatcga
      301 gcgcgacgcg cgcaacaatc ctgccatacg ctccaccccc atcagcatcg gcgaccacgc
      361 cgccatgctg ctgctccggg atgagtttgt gaagctcatg cgcgagcagc acgcccagtg
      421 gcaaaaaaac caggcccccg ccgccatgcg cgccatcttt gagacacaaa aagacgccgt
      481 cgccgcccgc cggtcgccgc tgtcctatgt gcgggtgcct gtgaagcaac tccagggtta
      541 cagcgagatc gagccttcca tgctgcaaaa agtctccctc tttttccgcg aggaggagga
      601 gccgctgctg cacacgcttt tcagcaggga attctccggc ggcgtcatgc gtgtggacaa
      661 ggacgctccc ggtttcgagc gcttgcagtg ggaggcgttc aagctggcct acacgggttg
      721 gggcgcgctc atgcagcgct ccatcactga cgcgcaaaac ctctccgatg gcgatgtgct
      781 cggcctgctc aagctcaccg ggatgagttt tgaacccgtc gtcgagcggc tgcggccgaa
      841 gctcatgaaa ctagaaattg acgctcaggc gcgccgccaa cgctgggtgc ccgaccgcgt
      901 cgcccgcgcc tacgtcaccg gcctgggcgt gctcggcgcg gccgtgcgcg cgcaggagga
      961 ggtgtcagag gccgacacgc gcgtcatcca ggccaccgcc gcgctcgcca gcgtggctct
     1021 gggcaatgtc agcggtgtct gggccgccgc agccgccgtg ggcatcagcg ctgcggacgc
     1081 cgcctatgcc atctaccagg acggctacat gaacaccgcg cggcgggacc agctccagtc
     1141 cgagatcggc gcaggcgcgg tgctcggagc ggatcgtgcg gcggaggctg agggcaaaat
     1201 cctctccacc ctcgagcaag cgctctccat cggcggctcc gcattcggcc tgacggcgga
     1261 tggcgcggtc ctgcgcagcc tgaagagcgc caaggcactg acgctggccg agacgctcga
     1321 taaagccgcc gacatcctca aaaacgtgga cgtggccgac cccgccgccc tgcgtaaact
     1381 cagcaaagcg gacgccgccg tcctgctgcg tgccgccgag gctgcggagg acgcggccat
     1441 cgcgggacgt cccatggact tgtttgaaaa gatccaggaa gtggtgcccc ataaagacga
     1501 cctgtttgag gcgctggccg agggcaccga ggcggcggga ctctctgaga agctgaaaag
     1561 agtggcggag tctccagcca tcaccttgaa gcggaccgag ctgcaggacg ctggcaaaaa
     1621 cctcgacgcg cgcatcgcca aaaccgaagc ggagatcagc gacatcaaag ccaaaaaacc
     1681 tgcggatgga tcagcggagg caaagctgct cgatgccaaa aaaggcgagc tgggcgatct
     1741 caagcagcaa aaaacggtcg tggatgccat ggagcagccc gtggtcagcg tgccgaagcg
     1801 caccgccgaa atgctcacgg gagaggccac cgccatcacc agcgccgagc gcgccgctgt
     1861 ggatgaggct tcgaagctgc tggccggtgg cggacgcttt agcggagagg gcttgcagaa
     1921 agtgaatctc acctccgcag gcaaacggct cgacgatctc atccagcaat ccacgaaacg
     1981 gctcgacgat cttcgcgcca atcccgctgc cgccaaagcc ggcgagcttg atgatgcggc
     2041 acgccggctt gatgatcttc agtccgcgcg cgaaactgtc cgaaagctcg acaaggccgt
     2101 tggcgacgca tctgagacac ggatttccca gcgcgacctc gatttgatca atggcgcgcg
     2161 gaaattcgac gatctcgacc aggcagaggt ccacaaagcc atcctcaaat ccggcggtga
     2221 ctggaagcag atcgtcagca aggccaagaa aggtcccgaa gagcaactgc tgctgcacaa
     2281 agtcgccgcc taccgtagaa aggtcgtcca tgagatcgcc gacgaggtca tcgagggcgt
     2341 cgaaaatgcg acaggacaaa aaggactctt tcgcgacgcc ttcggctccg ccaatctcag
     2401 cagcgactac gatgtggcct ttcgcggtcc cggcgcggag ctggccgtgc aggagtttaa
     2461 caaccgcgtg cgcaaggtcc tcggcggtcg cgagagcggc gcggtgctcg acaacaactt
     2521 ctacaccgac cctgtttaca gcatttacaa gctcggcgac ggcgcgggcg cgggcggagt
     2581 cacaccggcg cagttggaca ccgcgcgcca gtttctttac cagcagatgg ccggcgtcaa
     2641 ataccgcacc ccgaagcagt tcgcctcgtt ccgtaacaaa atgctcggcc agttgcccga
     2701 aggcccccgc aagttttttg acgacctcct cgatcaggcg ctcggcgcgc aatcgctcgc
     2761 gcaaaagcgc atcgaggcac gactcgccaa gatcggcaag acggcggaga cggccacacc
     2821 cggcgagatg ctgcgcgtca ccaatgacct ctatggggac accctctcag gcatccacgg
     2881 ctaccgcagc tttgtggacc atctcgatgc tgttatcagc ggcggcggca aatccctccg
     2941 caacgcgccc gtgccagcgg tgctgcaaaa cacggaattc tcgaagcagc tcgggatcat
     3001 ccaaggaatg atcgctgatc ccggcacgcg cgccgcaggt ctggaggcgc tgaaaaaact
     3061 gcgcgaccgc acggtgctga acatgcgcaa ccagcagggg ctggcgcttt actacgcctc
     3121 tgaggcttat caggcgggcg acacgatcaa gcacgtcgtc aaagagatcc aggcaggcgg
     3181 ccaccgcttt gaccccgttg ccgcgctgaa aggccagcgg ccatccaatc aaggcatcac
     3241 ccccgacggc tacatcaact cctactacga gaacggagcc aacctcgtca aggaactcaa
     3301 cggcaagcat gtgctggatg aggccacggg cgccctcaag tccggcctgg atccctccta
     3361 tctcgaagcc gcggcgacga agtctgccaa gtatttcgtc cgccagctcg acgccgccaa
     3421 cgccgccgga gcggacctca acaagctcac cgacgccgag cagcttctca ccgccaccgc
     3481 caggctcgac gccgcgcggg attcaccagc caagttcaac gacgtgctca aggagttcgg
     3541 ctggactcat gaggagtatg ttcaacgggt gctcgccgcg aatgactacc tcgactccca
     3601 ggtcgtcggc aagtccagcc tcacgggcat cgctggaaac ctcgtcgccg ctgaaaaaga
     3661 agccgccaac ctgcccgcgc ggctccaaga cctcgccagc cagagagctt atcaggaaag
     3721 cctcgtgcgg gtctttggag attcagccag tcttgccaaa ctcgagcgcc agtccctgaa
     3781 cgcgctcttc gacggccagc ccgcgactcc gccggacgcg cgctccgtct cggcgcaaaa
     3841 acaattcgaa gcatcaggcc aggccgcgcg cctcgccggt ctcagccagg aagaaatggc
     3901 cgggatcgtc gccgcggtgc cgcagagcgg tgatcccgcc gcttggtttg cgggttccgc
     3961 ctctgctttg gaaaaagcgg cgttggacag gctctcaagc agcccggcat ctccggcatc
     4021 cgcagccacc tccagcctgc caccggccac acgccgcgcc atc
//
LOCUS       DAOMYJ010000338         4060 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Prosthecobacter sp. isolate AMR_MDS_5629
            NODE_7841_length_4060_cov_17.621973, whole genome shotgun sequence.
ACCESSION   DAOMYJ010000338 DAOMYJ010000000
VERSION     DAOMYJ010000338.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867850
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prosthecobacter sp. (wastewater metagenome)
  ORGANISM  Prosthecobacter sp.
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Verrucomicrobiaceae; Prosthecobacter.
REFERENCE   1  (bases 1 to 4060)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4060)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.30
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:34:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,742
            CDSs (total)                      :: 4,684
            Genes (coding)                    :: 4,676
            CDSs (with protein)               :: 4,676
            Genes (RNA)                       :: 58
            tRNAs                             :: 55
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4060
                     /organism="Prosthecobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7841_length_4060_cov_17.621973"
                     /isolate="AMR_MDS_5629"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1965333"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2603
                     /locus_tag="PLP58_21280"
     CDS             <1..2603
                     /locus_tag="PLP58_21280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ11128.1"
                     /translation="AWLVTRPSPPAAEPQVAAVPALNGKAVVETPLTVPDSPPAPAPP
                     SPDPSSPEPPPVRPVIVEHDLLALTDPLMDRAPLPRLVRRNEWRREDGGGLAFQSDGQ
                     AGRLAAPVAFECADYEIEFKARKLEGSERVQLNLPAGEARELRLVFNSPNRKVMDGRE
                     GVPWRQIEELVHVSARIIHAGGGKDRVYIRRLDGAQQILADVTADIASLAADSRGHPA
                     FPGRPVLSVFLPKDAYRIETLRLRVFEGGAGVLREPGELPILTHGGSRWQFQPGAVSW
                     NEARDLAAARGGHLATITDQETYDWIKGEFISKQAPGLAVWLGGEKKPDTPVSDWAWV
                     TGESFAFRAFQPGAPSGTVPHFGLAWRRPPEADAAQGDSWWPANPGQSHDISGFLIEW
                     DEAPPASMPAPATVMEAPALPPDDFITKLESGFKARFDADAQTPFLAAVARLDQSYLA
                     NGLPRARAAAQARGSLAEVTALDEETARVGNKEGMPAEDVEGTPEALKTLRQTYRAAF
                     AKLAAERDARAAPLYDIYLKSLDGHIAVLTKAGKITEATAAQAWRDRIAERRPVNDSS
                     ARIAATQPAKAAPSVTAPKAAKASTPTGGGSSWRVAADYLVNNGGSFVSLKGGATITV
                     TKPDEIPSGRFDIIELSLDRLGSALPSPVDTDFAAFSGLRDLRRLWLRSIPALTDAAL
                     AFVAANDDLTFINFEGVNALTDAVTMHLEPLKKLDYLAIQYAEGFTGGGLGQLASKDS
                     MRTFEFLSSGITDEGMRAVGTFKKLQTLRVTSQKVTPAGFAPLAGLTSLTSLTLSGTQ
                     FDDTSADAVAGLLQLSHLDLANTKLGDAGLAKLKSLKKLSNLYLSGTAVSGKAAGEFQ
                     LALPQCRVSR"
     gene            complement(2697..3593)
                     /locus_tag="PLP58_21285"
     CDS             complement(2697..3593)
                     /locus_tag="PLP58_21285"
                     /inference="COORDINATES: protein motif:HMM:NF013429.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HRJ11129.1"
                     /translation="MNRRSFFQTTALAAAGLGFARPASAQSILKSAADPAYKIKNSGI
                     KHTLMGWCWKPMDMLELARHAKDIGLVGIEGIDRKLYPDVKKLGLGISLVGGHGFSNG
                     PCNPKFREEVIAKLTEGIDVAADVGCKKVITFTGMKFDGMDREQAVKDCLDTWKVVLP
                     HAEKKGVTLVLEHLNSRDDTHPMKGHPGYFGDDVDFCVDLIKQIGSPNFKLLFDIYHV
                     SIMNGDVIRRLRLHKDFIGHIHTAGNPGRCELDENQEINYPAVMKALLEIGYHDYVAH
                     EFIPTWEDPILALRHACMVCDV"
BASE COUNT          712 a         1338 c         1289 g          721 t
ORIGIN      
        1 tggcatggct ggtcacgcgg ccttcgccgc ccgcagccga gccgcaggtg gcggccgtcc
       61 ccgcgctgaa tggcaaggca gtcgtcgaaa cgccgcttac cgtgcccgac tcaccgcccg
      121 ctcccgcgcc gccaagtcct gatccttcca gccctgagcc tccgccagtg cgtccggtga
      181 ttgtggagca tgatctcctg gcgttgacgg atccgctgat ggaccgcgcg ccgctcccca
      241 ggctggtcag gcgcaacgaa tggcggcgcg aggacggggg aggtctcgcc tttcaaagcg
      301 atggccaggc gggcaggctg gccgcgccgg tcgcgttcga atgcgcggac tacgagatcg
      361 agttcaaagc gcgcaagctg gagggctccg agcgggtgca actgaacctg cccgccggcg
      421 aggccaggga gctgcggctg gtgttcaact caccgaaccg caaggtgatg gatggcaggg
      481 agggagtgcc atggcggcag attgaggagc ttgtgcatgt ctccgcgcgg atcattcatg
      541 cgggcggcgg caaagaccgt gtttacatcc ggcgcctgga cggggcgcag cagatcctcg
      601 cggatgtgac cgcggacatt gccagcctgg cagccgacag tcgcgggcat cccgctttcc
      661 cgggccggcc tgtgctttcc gtgttcctgc ccaaggacgc ctaccggatt gagacgctgc
      721 gcctgcgcgt gtttgaaggc ggggcaggcg tgctgcgcga gcctggcgag cttccgatcc
      781 tcacgcacgg tggcagccgc tggcagttcc agcccggcgc ggtctcatgg aacgaggcgc
      841 gtgatctcgc cgcggcgcgc ggcggtcacc tcgccaccat caccgaccag gagacttatg
      901 actggatcaa gggcgagttc atctcgaagc aggcgccggg cctggctgtc tggctgggcg
      961 gggaaaagaa gcccgatacg ccggtcagcg attgggcatg ggtgaccggt gagagtttcg
     1021 ccttccgcgc cttccagccg ggcgcgcctt ccggcaccgt gccgcatttt ggcctggcct
     1081 ggcggcgtcc gcctgaggcg gatgccgcgc agggtgactc atggtggccg gccaaccccg
     1141 gtcagagcca tgacatttcc gggttcctga tcgaatggga cgaggcgccg cccgcgtcca
     1201 tgccggcgcc cgccactgtc atggaagccc cggctttgcc gccggatgac ttcatcacca
     1261 aacttgaatc cggcttcaaa gcgcgcttcg acgccgacgc gcaaacgccc ttcctcgccg
     1321 ccgtcgcgcg cctggaccag agctacctgg ccaacggcct tccgcgcgcg cgagccgccg
     1381 cgcaggccag aggcagcctc gccgaagtca ccgcgctgga tgaggaaacg gcgcgcgtcg
     1441 ggaacaagga gggcatgccc gccgaggacg tggaaggcac gcccgaagcg ctcaaaacac
     1501 tgcgccagac ttaccgcgcg gcgttcgcaa agctcgccgc cgagcgcgat gccagggccg
     1561 cgccgctcta tgacatttat ttgaaatcgc tcgatggcca catcgccgtg ctgaccaagg
     1621 ccggcaagat caccgaggcc accgccgcgc aggcgtggcg ggaccgcatc gcggagcgga
     1681 ggcctgtcaa tgacagctcc gcccggatcg cagcgaccca gcccgcgaaa gccgcgccct
     1741 cagtcacggc acccaaggcg gcgaaagcat ccactccgac ggggggcggc agctcgtggc
     1801 gcgtggccgc ggattacctc gtcaacaacg gcggctcttt tgtgtccctc aaaggtggcg
     1861 ccaccatcac ggtcaccaag ccggatgaga ttccgagcgg gcgctttgac atcatcgagc
     1921 tttcgctgga ccggcttggc tccgcgctgc cgtcccctgt tgacacggac ttcgccgcgt
     1981 tcagcggtct gcgcgatctg cgccgcctgt ggcttcgcag tatccccgcg ctcacggatg
     2041 ccgcgctcgc ctttgtggcc gccaatgacg atttgacctt cattaatttc gagggcgtca
     2101 acgcactcac ggacgcggtc accatgcatc ttgagccgct gaaaaagctc gactacctcg
     2161 ccatccaata cgcggaaggc ttcaccggcg ggggactcgg ccaactggcc tcaaaggact
     2221 ccatgcggac ttttgagttc ctctccagcg gcatcaccga cgagggcatg agggctgtcg
     2281 gcaccttcaa gaaacttcag acgctgcgcg tcacctcgca gaaagtcacc cccgccggct
     2341 tcgcgccgct cgccggtttg acctcgctga ccagtctcac actcagcggc acgcagtttg
     2401 acgacacctc ggcggacgcc gtcgccggac tcctgcagct ctcgcatctc gacctcgcaa
     2461 acaccaagct cggtgacgcc ggcctggcaa aactgaagtc cctcaagaag ctctccaacc
     2521 tctacctttc cggcaccgcc gtcagcggca aggcggcggg ggagtttcag ctcgcgctgc
     2581 cccaatgccg cgtctcgcgc tgacggggtt ggtgttcagg ctttggctgg tgctggtcgc
     2641 attgtcggcc ttcccggcct tttctcaatg gccggctgat gcgcgcgcgt gagccctcac
     2701 acatcgcaca ccatgcaggc gtggcgcagg gccaggatgg gatcctccca ggtcgggatg
     2761 aactcgtggg ccacatagtc gtggtagcca atttcaagga gcgccttcat cacggcgggg
     2821 tagttgatct cctggttctc gtccagttcg cagcggccgg gatttcccgc cgtgtggatg
     2881 tggccgatga agtccttgtg gaggcgcagg cggcggatga catcgccgtt catgatggag
     2941 acatggtaga tgtcgaagag cagcttgaag ttcgggctgc cgatctgctt gatgaggtcc
     3001 acgcaaaaat ccacatcatc gccgaaatag ccggggtgtc ctttcatcgg gtgcgtgtca
     3061 tcgcggctgt tgagatgctc cagcacgagc gtgacaccct ttttttccgc gtgcggcagg
     3121 acgaccttcc aggtgtccag acagtccttg acggcctgct cgcggtccat gccgtcaaac
     3181 ttcatgccgg tgaaggtgat gaccttcttg cagcccacgt cggcggccac gtcaatgccc
     3241 tcggtgagct tggcgatgac ctcctcgcgg aacttgggat tgcagggtcc gttgctgaaa
     3301 ccgtgcccgc cgacgagcga gatgcccagg ccgagctttt tcacatccgg gtagagcttc
     3361 cggtctatgc cctcgatgcc aacgaggccg atgtctttcg catggcgcgc caactccagc
     3421 atgtccatcg gcttccagca ccagcccatg agcgtgtgct tgatgccgct gttcttgatc
     3481 ttgtaggcgg gatcggcggc ggatttcagg atggactgcg cggaagccgg gcgggcaaag
     3541 ccaagaccgg cggcggcaag agcggtggtt tggaaaaaag agcggcggtt catgagaaag
     3601 agaaaacagg aaacggcagg caaaggcaag acttcagggg aagttcgaaa ttcgaagccc
     3661 gattttcgac ctcctccacc cgccacgtca accacctgcg cccgccctcc cgccacaagg
     3721 ccgcatccgc cgcatccgca acaccggcgc gctcctcgcc gccctcgtta ttgctgtgcg
     3781 ggtcttcatc ggggttggtc gtctgcaaga ggtttggcga actcacaaac cacttccgag
     3841 actgaatccg gggaccacaa tccgcgagca agcagtgcca tgcagcacca cccatcacga
     3901 caaagcacat catccccgtg caaatccccg cgagaatggc ttgtgtgcgt gccacggcgc
     3961 ttcaggggtt tgggcaattt ctcggatcag gcgacgtcga gcgcaagacg ttgctgacac
     4021 gacagacagc cttcgagccg ttgcctgcat ccgttttgtt
//
LOCUS       DAOMYJ010000339         4053 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Prosthecobacter sp. isolate AMR_MDS_5629
            NODE_7865_length_4053_cov_19.660080, whole genome shotgun sequence.
ACCESSION   DAOMYJ010000339 DAOMYJ010000000
VERSION     DAOMYJ010000339.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867850
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prosthecobacter sp. (wastewater metagenome)
  ORGANISM  Prosthecobacter sp.
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Verrucomicrobiaceae; Prosthecobacter.
REFERENCE   1  (bases 1 to 4053)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4053)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.30
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:34:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,742
            CDSs (total)                      :: 4,684
            Genes (coding)                    :: 4,676
            CDSs (with protein)               :: 4,676
            Genes (RNA)                       :: 58
            tRNAs                             :: 55
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4053
                     /organism="Prosthecobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7865_length_4053_cov_19.660080"
                     /isolate="AMR_MDS_5629"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1965333"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..352
                     /locus_tag="PLP58_21290"
     CDS             <1..352
                     /locus_tag="PLP58_21290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ11130.1"
                     /translation="AAPAATAGGAMSSVPRDSGAIDATDEGSMEANFGLTTTVIGKVK
                     RVATLGGAGHKLIEFEDTQFNVFINKRQLDQSQDWHLEGLEGRLVQVKGKIAKYNDKL
                     QIQAYEPAQLGVVE"
     gene            complement(356..754)
                     /locus_tag="PLP58_21295"
     CDS             complement(356..754)
                     /locus_tag="PLP58_21295"
                     /inference="COORDINATES: protein motif:HMM:NF012790.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HRJ11131.1"
                     /translation="MLCSCKPQWHAPEPVSMPEVTLPDTVRKLSVEEASALIASTPGL
                     RILDLRMEEELARDGRFPKAAHVDFFHHARLAEHLAALEKDAPCLILCALGGRAEKVA
                     VRMHAAGFTRLHLLEGGIEAWIKAGQAMEK"
     gene            complement(897..2423)
                     /gene="modA"
                     /locus_tag="PLP58_21300"
     CDS             complement(897..2423)
                     /gene="modA"
                     /locus_tag="PLP58_21300"
                     /inference="COORDINATES: protein motif:HMM:NF024921.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009897 - external side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015412 - ABC-type molybdate transporter
                     activity [Evidence IEA];
                     GO_function: GO:0030973 - molybdate ion binding [Evidence
                     IEA];
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter substrate-binding
                     protein"
                     /protein_id="HRJ11132.1"
                     /translation="MKRLLGIILLLAAGTLAFFALKSGSKSSVDTLTVYCAAGLKKPV
                     EAAAAEYEKETGVKVALQFGGTGTLLTQLRVARQGDLFIAADDGALADARKLEVVREV
                     LPMVVQYPVLAVAKGNPKGIRTLDDVMKPDIRAALPNPEAASAGKVAQKLLGARWESL
                     AQKAAVMKPTVTEIAADVKLGAADAALVWNSTVPQFEGVEAVEVPEFSKHRENASAAV
                     LSSTVSSVEALKFARYLAAPEKGGQIFAAHGFTPAGGDKWAAKPELILYSGGVNRPAI
                     EHLVQEFANREGISVTTVFNGCGILCAAMKTMGDSSNPKFPDAYYACDVCFVPPVAEH
                     FPEAVLLTEAEIIIAVPKGNPKGIKTLADLARPGLRVGLCNAEQSTLGFMTMGILTSM
                     NLWDSVVANASSQVPTGDFLVNQLRAGSLDAAVVYQINIQHQAEHFDAIPLPADKAKA
                     IQPFSVRKDSPNRELARRLLDHLKANRESFEKAGFVWTGDNKPIKSADIELPEWLKQK
                     "
     gene            complement(2451..3431)
                     /locus_tag="PLP58_21305"
     CDS             complement(2451..3431)
                     /locus_tag="PLP58_21305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ11133.1"
                     /translation="MTRFFLPLLALTAHGTTCACTIPVFRYALDRWEADKFQLLLPTE
                     AGPETADLLRPLRANGKANLTIQAVPGVEGEAVLRFPSRQEIWRGRLNAASLAALLDS
                     PGRRRIVERVLAGDSVLWVVCGGEADTERIGKRLRFLEQVAALPIQDPNDPDSRLGPG
                     PPLLLKFTTLRLEQDDPAEKLLRRMLAGPRAEFDAEKTPFAAAVFGRGRVLGAWPLAE
                     LDDQALEEASMFLIGRCSCRVKDQNPGWDLLLNVDWDQALKKAGQNVAAAPPESAPPQ
                     APETVVTASKTSEPQVFVINQFVPLPWLAGGGALMLGAAAWLLLRKAPKD"
     gene            3544..3783
                     /locus_tag="PLP58_21310"
     CDS             3544..3783
                     /locus_tag="PLP58_21310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ11134.1"
                     /translation="MLSDAHAYLLAGFTEQEVREVLDDLDYLLQNSTWPYSRESTADM
                     IVELPSMLTDFLRSVRRDALQSAMISRKVKAAILG"
BASE COUNT          704 a         1394 c         1232 g          723 t
ORIGIN      
        1 agccgcgcct gccgcgacgg ccggcggggc catgtccagc gtgccgcgtg actcgggcgc
       61 gattgatgcc acggacgagg gttccatgga ggcgaacttc ggcctcacca ccaccgtcat
      121 cggcaaggtg aagcgcgtgg ccacgctcgg cggcgcgggg cacaagctca ttgagttcga
      181 ggacacccag ttcaatgtgt ttatcaacaa acgccagctc gaccaaagcc aggactggca
      241 tctcgaaggg ctggagggca ggctcgtgca ggtgaagggc aaaatcgcca aatacaacga
      301 caagctccag atccaggcct acgaaccggc ccagctcggc gtcgtcgagt gacgctcact
      361 tctccattgc ctggccggcc ttgatccatg cctcgatgcc gccctccagc agatgcagcc
      421 gggtgaatcc cgccgcgtgc atccgtaccg ccactttttc cgcccgccct cccagcgcgc
      481 acaggatgag gcagggcgcg tctttttcca acgccgccaa atgctccgcc agccgcgcat
      541 gatgaaaaaa atccacatgc gcggctttcg ggaaacgccc gtctcgcgcc agctcctcct
      601 ccatccgcaa gtccagaatg cgcaggccgg gagtggatgc aatcagcgcg gatgcctcct
      661 ccacagacag tttccgcacc gtgtccggca gtgtcacctc cggcatggaa accggctccg
      721 gcgcatgcca ttgcggcttg caggagcaca gcatgacaaa aagcaggatg caaaccggtg
      781 gacatggcag acgcatggcg gcagtctgcg ccctcctggc tgggcggcaa cggaatcttg
      841 ggtaagatcg taattcgaag atcgaggttt caattcgaat ttcgagcttt cctccctcac
      901 ttctgcttca accactcggg cagctcgatg tcggcgctct tgataggctt gttgtcaccc
      961 gtccagacaa agccggcttt ttcaaagctc tcgcggttgg ccttcagatg gtcgagcagg
     1021 cggcgggcga gttcgcggtt gggcgagtct ttgcgcacgg agaagggctg gatggccttc
     1081 gccttgtccg ccgggagagg gatggcgtcg aagtgctccg cctgatgctg gatgttgatc
     1141 tggtaaacca ccgccgcgtc gagcgagccg gcgcgcagtt ggttgacgag gaaatcgccg
     1201 gtcggcacct ggctggaggc gttggcgacg acgctgtccc acaggttcat ggaggtgagg
     1261 atgcccatgg tcatgaagcc gagcgtggac tgctccgcat tgcacaggcc gactcgcagg
     1321 ccggggcggg cgaggtcggc cagggtcttg atgcccttgg ggttgccctt cggcacggcg
     1381 atgatgatct ccgcctcggt cagcagcacg gcctccggga aatgctcggc cacgggcggg
     1441 acgaagcaga catcgcaggc gtaataggca tccgggaact tggggttgct gctgtctccc
     1501 atcgtcttca tggccgcgca caggatcccg cacccgttga agacagtggt gacgctgatg
     1561 ccctcgcggt tggcgaactc ttgcaccaga tgctcgatgg ccgggcggtt caccccgccg
     1621 ctgtagagga tgagttccgg cttggcggcc catttgtctc cgcccgcagg ggtgaagcca
     1681 tgcgccgcga aaatctgccc gcctttttcc ggcgccgcga ggtagcgggc aaatttcagc
     1741 gcctccaccg aggagactgt cgagctcagc acggcggcgc tggcgttttc gcggtgcttg
     1801 gaaaactccg gcacctccac cgcctccacg ccttcaaact gcggcacggt gctgttccag
     1861 acgagtgccg cgtccgccgc gccgagcttc acatcggcgg cgatctcggt gacggtgggc
     1921 ttcatcaccg ccgctttttg cgccagcgac tcccagcgcg cgccgaggag cttttgcgcc
     1981 accttgcccg cgctggcggc ctcgggattc ggcaaggccg cgcggatgtc cggcttcatc
     2041 acgtcgtcca gcgtgcggat gcccttgggg ttgcccttcg ccacggcaag cacggggtat
     2101 tgcaccacca tgggcagcac ttccctcacc acctccagct ttcgcgcgtc cgccagcgcg
     2161 ccgtcatcgg cggcaatgaa aagatcgccc tgcctggcca cgcggagctg cgtgagcagc
     2221 gtgcccgtgc cgccgaattg cagcgccacc ttcacaccgg tttctttttc atactctgcg
     2281 gccgctgcct ccacgggctt tttcaatccg gccgcgcaat acacggtgag cgtgtccacg
     2341 ctggacttcg agccgctttt caaggcgaaa aaggcgagcg tgccggctgc aagcaggagg
     2401 ataatgccaa ggagtcgttt catgagagtc aaagtcggag cgggagaggg tcaatctttc
     2461 ggggccttgc gcaagagcag ccaggcggcg gctccgagca taagcgcccc gccgccggcg
     2521 agccagggca aggggacgaa ctggttgatg acgaagactt gaggttcgga agtcttggaa
     2581 gcggtcacca ccgtctccgg tgcctgcggc ggggcgctct ccggcggtgc cgccgccacg
     2641 ttttgccccg cttttttcag cgcctggtcc cagtccacat tcagcagcag atcccagccg
     2701 gggttttggt ccttcacgcg gcaggagcag cggccgatga gaaacatgct cgcctcctcc
     2761 agcgcctggt cgtccagttc cgccagcggc cacgcgccca gcacgcgccc gcgcccgaag
     2821 acggccgcgg cgaagggcgt cttctccgcg tcgaactccg cgcgcggccc cgccagcatc
     2881 cggcgcagca gcttttccgc ggggtcatcc tgctccagcc gcagcgtggt gaatttcaaa
     2941 agcagcggcg gccccggccc gagacggctg tccgggtcgt tcgggtcctg gatgggcagg
     3001 gcggccacct gctccagaaa gcgcaacctt ttgccgatgc gctccgtgtc cgcctcaccc
     3061 ccgcacacca cccacagcac cgagtcgccc gccagcacgc gctccacgat ccgccgccgc
     3121 cccggcgagt ccagcagcgc cgccagcgag gccgcattta ggcggccacg ccagatttct
     3181 tgccggcttg gaaaacgtag tacggcttct ccctcgacac cgggcacggc ctgaatggtc
     3241 agattcgcct ttccattcgc cctcagcggg cgcagcagat ccgccgtttc aggccccgcc
     3301 tctgtcggca gcaacagttg aaatttgtcc gcctcccacc ggtccagcgc atagcggaag
     3361 accgggatcg tgcatgcgca cgtcgtgccg tgcgcggtca gggccagcag ggggaggaaa
     3421 aagcgcgtca ttgggcgatt gggaacggag ccccgcagga aaagttgccg ccaccttggt
     3481 gtccaggctt tagccgcccc gccacgcagt tgacgcccgc gcccctccgg ctatctgcac
     3541 gccatgcttt ccgacgccca tgcctacctc ctcgccgggt tcaccgagca ggaggtccgc
     3601 gaggtgctcg acgacctcga ctacctcctg caaaactcca cctggcccta cagccgcgag
     3661 agcacggcgg acatgatcgt cgaactgccc tccatgctca ccgacttcct ccgctccgtc
     3721 cggcgggatg ccctccagag cgccatgatc tcccgcaaag tcaaggccgc catcctcggc
     3781 tgaccatcac ccttcgccaa aatgcccctc cgccctttac agccaggggg caccgtgcaa
     3841 gatgcagcgc tttcagggca gtaggacagg catcctgcct gtctcgtgtg ttccgggcat
     3901 tctgcccgga acacccaatg cccacgaaag tgcccccacc tgaacgcttt cacccccagc
     3961 attcgacatt cgtcattctg cattcgtcat ccccccatca tgcccaaaga cacctccatc
     4021 aaacgcattc tcgtcatcgg ttccggcccc atc
//
LOCUS       DAOMYJ010000340         4045 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Prosthecobacter sp. isolate AMR_MDS_5629
            NODE_7890_length_4045_cov_20.011278, whole genome shotgun sequence.
ACCESSION   DAOMYJ010000340 DAOMYJ010000000
VERSION     DAOMYJ010000340.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867850
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prosthecobacter sp. (wastewater metagenome)
  ORGANISM  Prosthecobacter sp.
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Verrucomicrobiaceae; Prosthecobacter.
REFERENCE   1  (bases 1 to 4045)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4045)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.30
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:34:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,742
            CDSs (total)                      :: 4,684
            Genes (coding)                    :: 4,676
            CDSs (with protein)               :: 4,676
            Genes (RNA)                       :: 58
            tRNAs                             :: 55
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4045
                     /organism="Prosthecobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7890_length_4045_cov_20.011278"
                     /isolate="AMR_MDS_5629"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1965333"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..465)
                     /locus_tag="PLP58_21315"
     CDS             complement(<1..465)
                     /locus_tag="PLP58_21315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009961565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1501 domain-containing protein"
                     /protein_id="HRJ11135.1"
                     /translation="MKMHPNCQGNHIDLRIGASRRDFMYVGLLGGLGLTLPEMLRLQA
                     AQVMPEVETFKPIADSIIHIYLPGGMAQHESWDPKPFASPDYRGPYTPIKTSVPGEYV
                     GEKFQNIAKIMNKLTVIRSMTHGEAAHERGTHNMFTGYRPSPAIKFPSFGSII"
     gene            complement(556..921)
                     /locus_tag="PLP58_21320"
     CDS             complement(556..921)
                     /locus_tag="PLP58_21320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ11136.1"
                     /translation="MTQSTNDPHGGKGEPDRLAREPLVLMRKDPEVAGMRRKLLWLAA
                     LILGAVLLAVLNHGMKPASNSGSTVELRASKSSPKSAIPHLLPASEGNLEPDIMESFK
                     RAQSGGPPGFDALSQPPGL"
     gene            complement(975..2282)
                     /locus_tag="PLP58_21325"
     CDS             complement(975..2282)
                     /locus_tag="PLP58_21325"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HRJ11137.1"
                     /translation="MHRWTYLMLALLACAPLSCKRVEAKLDRLARAAGLIEEPPPPKP
                     AEPVLTPEELALEQRLQDGAAFATATQDDAVPKAVPFELNKSSVVSILGYHDFRERGG
                     SPMIIAVDKFREQMKAVKDSGIPVIPLSDVLAWRRGEKNIPEEAFVITMDDGWQGVYE
                     HAFPILKEHGFPFTIYLYKKYVNAGGRSMTWAQIKEMMEHGCEVGSHSVSHESLRARK
                     KGRTEEQHQEWVLAELKESREFIEAALGVPCRTHAYPFGIFDDAIAETGLQVGYEALV
                     TVNSQKVVWDTPLSKMGRFIIHGDSNTNFKLATSFRGRGDVSSSHFLAADMRDAEGKK
                     LVELSPEPEAVIKERLPLIRARLAGVGTVVPESVRLRVAGLGMVPAVYDTGSMTVSYQ
                     IPVRLRREDCAVTLSFKKAADQPDEVLTWRFKVDLAAEYAPRM"
     gene            2277..3311
                     /locus_tag="PLP58_21330"
     CDS             2277..3311
                     /locus_tag="PLP58_21330"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015411.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HRJ11138.1"
                     /translation="MHGLLIKPCPAKASRQPPSRAAAAERPAFQACPLAANLQTARPN
                     PSTHLMSSQKIAFVGVGRMGANMARRLKDCGHILSAVFDVNRPAAESLAAELGCSAPA
                     TLREVTAASDLIFTVVTNDDAMRGIFFNEEDNLLIGAEGRVFLNCATLSPGIHREVAA
                     AAAKAGAACLEAAMASSISHAREGKLYLMIGGDEAAYQKVLPILEQMSVNRRHVGAIG
                     RAAEVKALVNMVMNINTAGLAEGLGLAAALGHDLGMIREVFSQTGANSRVLETDSADM
                     AAREHDCWFSAEHAAKDSGIAAGLAAEAGLNLPLNNAAKAQYDKMVALGLGGLDKSGI
                     AELTFKGRHA"
     gene            3363..>4045
                     /locus_tag="PLP58_21335"
     CDS             3363..>4045
                     /locus_tag="PLP58_21335"
                     /EC_number="3.4.21.105"
                     /inference="COORDINATES: protein motif:HMM:NF013827.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HRJ11139.1"
                     /translation="MSLDNRDYMRNEPRPAEGALFSRMDTFQWVFALNIGVFVIQWLF
                     QIGWIEDPVTGRRVMPMGGVSVDDLARGQVWTPFTYMFVHGSVGHILVNMIMLWFAGR
                     RVHDLFGGRNFLYIYLMSGLAGAALEMAQGALVLGSTSITLIGASACCMGLLLAYALA
                     MPDEQLTLLLFFIIPVQATLRGMARFLLLLNVALGLLACFDALPSWLSGGGGAVAYWA
                     HVGGALAGW"
BASE COUNT          667 a         1253 c         1352 g          773 t
ORIGIN      
        1 gatgatggag ccgaagctgg ggaacttgat ggccgggctg gggcggtagc cggtgaacat
       61 gttgtgggtg ccgcgctcgt gggcggcctc gccatgagtc atgctgcgga tgaccgtcag
      121 cttgttcatg attttggcga tgttctggaa tttctcgccg acatattcgc cggggacgga
      181 ggtcttgatg ggcgtgtagg ggccgcgata gtccggggag gcgaacggtt tcggatccca
      241 ggattcgtgc tgggccatgc cgccgggcag gtagatgtga atgatggagt ccgcgatggg
      301 cttgaaggtc tccacttcgg gcatgacctg ggcagcctgg agacggagca tttcaggcag
      361 ggtgaggccg aggccgccaa gcaggccgac gtacatgaag tcgcgccggc tggcgccgat
      421 gcggaggtca atgtggttgc cttggcagtt ggggtgcatt ttcatgggag tgagggggta
      481 tgttttttcg gcggggattt acggctgccg ttcagccttc ttgcagtttt gaaaaaaatt
      541 cttccgtggc tggtttcaca agcctggcgg ttgtgacagg gcgtcgaaac ccggggggcc
      601 gccggactga gcgcgcttga aggactccat gatgtcgggt tccaggttgc cttcagaggc
      661 agggagcagg tgcgggatgg cggatttggg cgaggatttg gaggcgcgga gttccactgt
      721 ggagccggaa ttggatgcag gtttcatgcc atgattgagc actgccagca ggaccgcgcc
      781 caggatgagg gcggccagcc agagcagttt ccgcctcatt ccggccactt ccgggtcttt
      841 ccgcatcagc accagaggct cgcgcgccag cctgtccggc tcgcccttcc cgccatgcgg
      901 atcgttcgtg gactgtgtca tggtgatttc taaaacatac ccggtctggg cgggaaatct
      961 tgccgggagt tggatcacat gcgcggggcg tattcagcgg cgaggtccac tttgaaacgc
     1021 caggtcagga cttcatcggg ctggtcggcg gcttttttga aactgagggt cacggcgcaa
     1081 tcctcccggc gcaggcggac gggaatctgg tagctcacgg tcatggaccc ggtgtcataa
     1141 accgccggca ccatgccaag gccggccaca cgcaggcgga cggactcggg caccacggtt
     1201 ccgactcccg ccagccgcgc ccggatgagg ggcaggcgct ccttgatgac ggcctcaggc
     1261 tcgggagaca attccacgag ttttttcccc tccgcgtccc gcatgtcggc ggccaggaaa
     1321 tggctggagg acacatcgcc gcgcccgcga aagctggtgg cgagcttgaa gttggtgttg
     1381 ctgtcaccgt ggatgatgaa gcggcccatt ttggacaggg gcgtgtccca gacgactttt
     1441 tgcgagttga ccgtgacgag cgcctcgtag ccgacctgga ggccggtctc cgcgatggcg
     1501 tcgtcgaaaa tgccgaacgg ataggcgtgc gtgcggcagg gcacgccgag tgcggcctca
     1561 atgaactccc tggactcttt caactcggcg agcacccact cctggtgctg ctcctcggtg
     1621 cggccctttt tgcgcgcgcg caggctctcg tgggagacgc tgtggctgcc cacctcgcag
     1681 ccgtgctcca tcatctcctt gatctgcgcc caggtcatgc tgcggccgcc ggcgttcaca
     1741 tactttttgt aaaggtagat ggtgaaggga aagccgtgct ccttgaggat ggggaaagcg
     1801 tgctcgtaaa cgccctgcca gccgtcatcc atggtgatga cgaaggcctc ctccgggatg
     1861 tttttctccc cgcgcctcca ggccagcaca tcactcagcg ggatgaccgg gatgcctgag
     1921 tccttgacgg ccttcatctg ctcgcggaat ttgtccacgg cgatgatcat ggggctgccg
     1981 ccgcgctcgc ggaagtcgtg gtagccgagg atggagacga ccgaggattt gttcagttcg
     2041 aacgggacgg ccttcggcac cgcgtcgtcc tgcgtggcgg tggcgaaagc cgcgccgtcc
     2101 tggagccgct gctccaaggc cagctcctcg ggcgtgagca cgggctcggc gggcttgggc
     2161 ggaggcggct cctcaatgag acccgcagcc ctggccaggc ggtcgagctt ggcttcgacg
     2221 cgcttgcagg agagcggagc gcaggccagc agtgcgagca tgaggtaggt ccaacggtgc
     2281 atgggcttct cataaagcca tgccccgcaa aagcaagccg ccagccccct tcccgggcgg
     2341 cagcggcgga gcggcccgct ttccaggctt gccctttggc cgcgaatctc caaactgcgc
     2401 gtcccaaccc ttccacacac ctcatgagca gccagaaaat cgcctttgtc ggagtcggac
     2461 gcatgggggc caacatggcc cgccgcctta aagattgcgg acacatcctc agcgccgtct
     2521 ttgacgtgaa ccggcccgcc gccgaatcgc tggccgccga gctgggctgc tccgccccgg
     2581 ccacattgcg cgaggtcacc gccgcctcgg atctcatttt caccgttgtc accaatgacg
     2641 acgccatgcg cggcatcttt ttcaacgaag aggacaatct tctcatcgga gcggaagggc
     2701 gcgtttttct caactgcgcc accctttccc ccggcatcca ccgcgaggtg gccgccgccg
     2761 ccgcgaaagc cggggccgcc tgcctggagg cggcgatggc ctcctccatc agccacgcgc
     2821 gcgagggcaa gctctacctg atgatcggcg gcgacgaggc ggcataccaa aaagtgctcc
     2881 ccatcctgga gcaaatgagc gtgaaccgcc gccatgtggg cgccatcggc cgcgcggcgg
     2941 aggtgaaggc gctggtgaac atggtcatga acatcaacac cgccggcctg gccgagggcc
     3001 tcggactcgc cgccgctctt ggacacgacc tgggcatgat ccgcgaggtc ttcagccaga
     3061 ccggagccaa cagccgcgtg ctggagactg acagcgccga catggcggcg cgcgagcacg
     3121 actgctggtt ctccgccgag catgccgcca aggacagcgg catcgccgca gggctggccg
     3181 cagaggcggg actgaacctg ccgctgaaca atgccgccaa ggcccagtat gacaaaatgg
     3241 tggccctcgg cctgggcgga ctcgacaagt ccggcatcgc cgagctgacc ttcaagggcc
     3301 gccacgcctg aggcggaaaa tttgcctcgc aaggccgcgc ccgcgcccgt agtatcgtcc
     3361 ccatgtccct ggacaaccgc gattacatgc gaaacgagcc gcgccctgcg gagggcgcct
     3421 tgttttcacg catggacacc tttcaatggg tcttcgcgct gaacatcggt gtcttcgtga
     3481 tccagtggct ttttcaaatt ggctggatcg aggacccggt cacaggccgc cgcgtcatgc
     3541 ccatgggtgg agtcagtgtg gatgatctgg cgcgcgggca agtgtggacg cccttcacct
     3601 acatgttcgt ccacggcagt gtgggccaca ttctggtgaa catgatcatg ctctggttcg
     3661 ccgggaggcg cgtgcatgac ctcttcggcg ggcgcaactt cctttacatc tacctcatgt
     3721 ccggcctggc cggggccgcg ctggaaatgg cgcagggagc gctggtgctg ggcagcacct
     3781 ccatcacgct catcggcgcc tcggcctgct gcatggggct gctcctggcc tatgcgctgg
     3841 ccatgccgga cgagcagctc accctgctgc tgttcttcat catccctgtc caggccaccc
     3901 tgcgcggcat ggcgcgtttc cttctcctcc tgaatgtggc gctcggcctg ctggcctgct
     3961 tcgacgccct gccctcctgg ctctccggcg gcggcggcgc ggtggcctac tgggcgcatg
     4021 tgggcggcgc gctggccggg tggta
//
LOCUS       DAOMYJ010000341         4035 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Prosthecobacter sp. isolate AMR_MDS_5629
            NODE_7910_length_4035_cov_23.928894, whole genome shotgun sequence.
ACCESSION   DAOMYJ010000341 DAOMYJ010000000
VERSION     DAOMYJ010000341.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867850
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prosthecobacter sp. (wastewater metagenome)
  ORGANISM  Prosthecobacter sp.
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Verrucomicrobiaceae; Prosthecobacter.
REFERENCE   1  (bases 1 to 4035)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4035)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.30
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:34:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,742
            CDSs (total)                      :: 4,684
            Genes (coding)                    :: 4,676
            CDSs (with protein)               :: 4,676
            Genes (RNA)                       :: 58
            tRNAs                             :: 55
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4035
                     /organism="Prosthecobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7910_length_4035_cov_23.928894"
                     /isolate="AMR_MDS_5629"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1965333"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..762
                     /locus_tag="PLP58_21340"
     CDS             <1..762
                     /locus_tag="PLP58_21340"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009957898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoamide acetyltransferase family
                     protein"
                     /protein_id="HRJ11140.1"
                     /translation="APAGGASSARGPATPAIRPSYGPEDERVPTSSMRNIIAERLLAS
                     KTQIPHFYLQMEVDAGPLMDFRAHLNASAEKAGGNKYTVNDFILKAVIRAAQAQPAIN
                     AAWDGDAIVKFKSVGLSVAIAIDDGLVTPVIKQAEKKTLLEISQAVKDLAGKAKNKKL
                     SPDDFAGGTITVSNLGAYGIDQFAAIINPPQAAIVAIGSIRKAPVVDDKGQITVGQRM
                     WVGLSGDHRVVDGAVAATFLAEMRKLLENPALMLV"
     gene            1296..>4035
                     /locus_tag="PLP58_21345"
     CDS             1296..>4035
                     /locus_tag="PLP58_21345"
                     /inference="COORDINATES: protein motif:HMM:NF024353.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter-associated beta strand
                     repeat-containing protein"
                     /protein_id="HRJ11141.1"
                     /translation="MTTSPFTPTPRLLATAPLLASFLLLGQTHATAATIQWTGTDSAL
                     WAAGANWSGGSAPVAVDDIVFGSPVPGTGPIITLASGSLANRLWVRDDYTLSGGDVSL
                     TAGSIEVNLGNTLTLDSLLAGSAGLSKTRGGTVRLTNNSNSYTGITSIANGTLVISHP
                     TQLGSDPSAVVISESNTAAGSNSIIGFYGGSLVLDGSLGGMNFTRDVHLQGSGPIADR
                     GGALISIGDNTLSGALRMADGASPRSSRILSNNGTLMLSGGLNAAGTPGTHISSLGGV
                     NTAGAGGSFVLTGELTGSGTLQKQGAGTLVLTPSSTAGFEGRLRISGSATGGQSSVRV
                     FAGDVFGTANSGNASAPIDMNGGVLEIHSDSGLDIAKNVYNRASSTYFFAPAIGGDGI
                     NGNVTFGTLRAAANTTATFNSRNGFGATFGTQSMESSNNNSTFTNNMGGPLVFTGNFW
                     NNSDGSARTLTISGSGNTVIQGSINTSGAGEKTITKTNNGVLTLNGTATSLNGNVNIS
                     GGAVAVRDFRALNNASPGIVNIGSGGTAGALIIGTSAPADSEGLRTTRTINLASTTGA
                     PSIYANQSGLHPVVLAGAFTTTGGSATQSKTLVLGGTNTADNIIHGGISNQAAATTGA
                     VSLLKSGPGTWVLAGENTYTGTTTISNGTLKLRANAPASTVLGAANNITFGANNSYAG
                     GTLEFAGQAGQSNMQSLGVMAYSAGSNTLRVTPGAGGTASLVIDRLNTTGGSTLNIVG
                     ADFVSNLVTFTTINGSVGSDGILTRSVYWNGADFAFREGGVARAPVYGVDVGMVTSDT
                     ELAAGNNQITGSFATGSISVSTLKILGSRTLTINDGATLTLSGGGLLATHGSSTVTGG
                     TLALGSQTLVARVNESADQLAIESVVTGTGGFTKSGAGTLILSGASTRTGTTSIDEG"
BASE COUNT          846 a         1290 c         1205 g          694 t
ORIGIN      
        1 gcccccgccg gcggagccag ctccgcgcgc ggccccgcca cacccgccat ccgccccagc
       61 tacggccccg aggacgagcg cgtgcccacc agcagcatgc gcaacatcat cgccgagcgc
      121 ctgcttgctt ccaagaccca gatcccccac ttctaccttc agatggaagt ggacgccggc
      181 ccgctcatgg acttccgcgc gcacttgaac gcctccgccg aaaaagccgg cggcaacaaa
      241 tacaccgtca acgacttcat cctcaaagcc gtcattcgcg ccgcccaggc ccagcccgcc
      301 atcaatgccg cctgggatgg cgacgccatc gtcaaattca aatctgtcgg cctcagcgtc
      361 gccatcgcca ttgacgacgg tctcgtcacc cccgtcatca agcaggctga aaagaaaacc
      421 ctcctggaga tcagccaggc cgtcaaagac ctcgccggca aagccaaaaa caaaaagctc
      481 agccccgacg acttcgccgg cggcaccatc accgtctcca acctcggcgc gtacggcatt
      541 gaccagttcg ccgccattat caatcccccc caggccgcca tcgtcgccat cggcagcatc
      601 cgcaaagccc ccgtcgtgga tgacaaaggc cagatcaccg tcggccagcg catgtgggtc
      661 ggcctcagcg gcgaccaccg cgtcgtggac ggcgccgtcg ccgccacctt cctcgccgaa
      721 atgcgcaagc tcctggaaaa cccggcgctc atgctggtct gagtggaggt tcggaaatct
      781 ggactcggtt tcacaccgca ctgcctgcgg cggcacgcgc gccatcccgc gcggattcgg
      841 ttctggactg gaatgcggcc atgccttcgc gcggctcgtt gacggctcgc aagacgctcc
      901 aaatgagggt ggaaacacac tcctccaaat tggcgaaaaa aggcgaaaaa aggcggaaaa
      961 ttatggctcg gaactctcat tgactaggaa gatgaatgaa caagaaagcc ggggaggctg
     1021 gctgcgcgga taaagtccgg gcgtctttaa gatacgagaa acctttttga caaaggcggc
     1081 tttggaattc caaaggcggt gattctcaga agccggacgg acacgcgcag gcgatgcaaa
     1141 cacaggccat cgctggcttt ttgatgcctg gcggacggcc gctttccgtg gatacgcaaa
     1201 gactttagcg tgccgggcag ggcgaggtgg cggggttatt ttttgactcg cccgtctcat
     1261 gggcaggttc ctatgacggc gaaaatccaa tcgccatgac caccagtccc ttcactccga
     1321 ccccgcgcct gctcgccacc gctccattgc ttgcctcgtt tttgctgttg gggcagactc
     1381 atgcaaccgc agcaaccatt caatggacgg gaacggacag cgcgctatgg gctgcgggcg
     1441 ccaattggag tggtggaagc gcgcccgtcg cagttgatga cattgttttt ggttcgcctg
     1501 ttcctggcac cggcccgatc atcactcttg catccggcag tctggcgaac agactttggg
     1561 tgcgtgatga ctacacctta tccggcgggg atgtcagcct cactgccgga agcatcgagg
     1621 tgaatcttgg aaacacgctc acgttggact cactcctggc tggaagcgcg gggctgagca
     1681 agacgcgcgg tggcaccgtg aggctcacga acaattcgaa ttcctacaca gggattacaa
     1741 gcattgcgaa tggaaccctg gtcatcagcc acccgaccca gcttgggtca gacccctccg
     1801 ccgtggtcat cagcgagtcg aacacggccg ccggcagcaa ttcgatcatc ggattctatg
     1861 gcggctcttt ggtgctcgat ggcagcctgg gggggatgaa tttcacaagg gatgtccatc
     1921 ttcagggcag cggccctatt gctgacaggg gcggcgccct catcagcatt ggtgacaaca
     1981 cactctccgg agccttgaga atggcggatg gggcatcccc gcgctcttct cgaatcctct
     2041 cgaacaacgg cactctgatg ctttccggcg gattgaatgc ggcgggaaca cccggcactc
     2101 acatcagcag cctgggcgga gtgaacacgg cgggggctgg cggctccttc gtcttgacgg
     2161 gggagttgac cggcagcggc acgctgcaaa aacagggggc ggggacactg gtcctcacac
     2221 catcctccac agcgggtttt gaagggcgac tgcggatatc cggcagcgcc acaggcgggc
     2281 agagctccgt gcgcgtcttt gccggcgatg tttttggaac ggcgaactcg gggaacgcgt
     2341 cggcaccgat tgacatgaac ggcggcgttt tggagatcca ctccgacagc ggtctggaca
     2401 tcgcgaagaa tgtttacaac agagcgtcct ccacctattt cttcgctccg gcgatcggcg
     2461 gtgacggcat caatggcaat gtgactttcg gcacactacg cgccgcggcg aacaccacgg
     2521 ccaccttcaa cagccgtaac ggattcggcg ccacgtttgg cacgcagagc atggagagct
     2581 cgaacaacaa ctcgaccttc accaacaaca tgggaggccc gctggtgttc accgggaact
     2641 tttggaacaa cagcgacgga agcgcccgca ccctcaccat cagcggcagt ggcaacacgg
     2701 tgatccaggg cagcatcaac acgagcgggg ccggtgaaaa aacgatcacc aaaaccaaca
     2761 atggcgtgct gaccctgaac ggcaccgcca cgtcgttgaa tggtaatgtc aacatcagtg
     2821 gcggtgcggt ggcggtccgt gattttcgcg cgctcaacaa cgccagtcct gggattgtca
     2881 atatcggttc aggcggcacg gccggcgcgc tcatcatcgg cacatccgcg ccggcggact
     2941 ccgagggttt gcgaaccacg cgcaccatca acctggcctc caccacagga gcgccaagca
     3001 tttacgcgaa ccagtccggc ctgcaccccg tggtgctcgc cggcgcgttc accacaactg
     3061 gaggcagcgc cactcagagc aagacgctcg tccttggcgg cacgaacacg gcggacaaca
     3121 tcatacacgg gggcatcagc aaccaggctg cggcgacaac cggcgcggtg agcctcctga
     3181 aatccggccc gggcacctgg gtgctcgcgg gagagaacac ctacaccggc accacaacga
     3241 tctccaacgg gacactgaaa ctgcgcgcga acgccccggc ctcgacggtg ctgggcgctg
     3301 caaacaacat cactttcggc gcgaacaaca gctatgccgg cggaacgctg gagtttgccg
     3361 ggcaggcggg tcagagcaac atgcaaagcc tgggcgtcat ggcatactcc gccggctcga
     3421 acacgctgcg ggtcacgccg ggcgcgggag gcacggcgtc attggtgatt gaccggctca
     3481 acaccacggg gggcagcacg ctcaacattg tcggcgctga ttttgtaagc aacctcgtca
     3541 cattcacgac gatcaacggc tctgtgggca gtgacggcat cctcacccgt tcggtttact
     3601 ggaacggcgc tgattttgcc tttcgcgaag gcggggtggc gcgcgcgccg gtttatggtg
     3661 tggacgtcgg catggtgaca tcggacacag agctggccgc ggggaacaac cagatcacag
     3721 gcagttttgc caccggatcc atatcggttt caaccttgaa gatactggga tctcggacgc
     3781 tgaccatcaa tgacggggcc actcttacct tgtcaggcgg cgggttgctt gccacgcacg
     3841 ggagttccac cgtcacaggc ggcaccctgg ccctgggcag ccagacgctg gtcgcgcgtg
     3901 tgaacgagtc cgcggatcaa ctggccattg aatccgtggt cactgggacc gggggcttta
     3961 ccaaaagcgg cgcgggcact ttgatcctgt ccggcgccag cacgcgcacg ggaacaacca
     4021 gcattgacga gggtg
//
LOCUS       DAOMYJ010000342         4003 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Prosthecobacter sp. isolate AMR_MDS_5629
            NODE_7997_length_4003_cov_19.291793, whole genome shotgun sequence.
ACCESSION   DAOMYJ010000342 DAOMYJ010000000
VERSION     DAOMYJ010000342.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867850
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prosthecobacter sp. (wastewater metagenome)
  ORGANISM  Prosthecobacter sp.
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Verrucomicrobiaceae; Prosthecobacter.
REFERENCE   1  (bases 1 to 4003)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4003)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.30
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:34:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,742
            CDSs (total)                      :: 4,684
            Genes (coding)                    :: 4,676
            CDSs (with protein)               :: 4,676
            Genes (RNA)                       :: 58
            tRNAs                             :: 55
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4003
                     /organism="Prosthecobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7997_length_4003_cov_19.291793"
                     /isolate="AMR_MDS_5629"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1965333"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(48..1160)
                     /locus_tag="PLP58_21350"
     CDS             complement(48..1160)
                     /locus_tag="PLP58_21350"
                     /inference="COORDINATES: protein motif:HMM:NF024989.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Zn-dependent alcohol dehydrogenase"
                     /protein_id="HRJ11142.1"
                     /translation="MPTAPAAVLYESAQPMVIEEVEVLPPQAGEVMVRMKAAGVCHSD
                     LHVMKGDLPMPVPIILGHEGAGVVEEAGPGVTSVAPGDHVVPIWRASCGRCDFCLKGR
                     PALCDMGTAMRFTGLMPDGNTRFRSAGGRSIRHYAGVSTFSSLSTMPEAAVVRIDPAF
                     PLWKAALISCGVITGVGAVTHAAKVPPGSSVAVFGCGGIGLNIIQGARMVSAGPVIAV
                     DAHPGKEAHARRMGAAHFINAAECDAVQAVKDLTGGRGVDYAFEAIGQPRAIEQAYDS
                     LKKGGTCVVSGICRADARAAINVNQLVYAEKTLRGTLYGSTRPRIDVVNLMRLHESGA
                     LQLDELLTRTWRLEQINEAYEALERGEVARSLIVWE"
     gene            2687..3775
                     /locus_tag="PLP58_21355"
     CDS             2687..3775
                     /locus_tag="PLP58_21355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ11143.1"
                     /translation="MPNPAGARYDDGLTYWDDGSTYDSATAAQPPLLTPLQPISPSTT
                     MEFWEVTKNRAQETLPVWASYAPDLTVGGKTPADLEALIDGFEPLVQARTLAQDGVDA
                     AYYAIQDALQRMRVLGTRVPQIIEGQLDENALLMKDVDDLFRTSPRTEGTILKRLRDL
                     LPVWERANTALAAMTPPQPPVTRTVGGVAYTVASATTLLDGYTALVKAHKDKEEDLDI
                     TRQELAAHDRACDQLNKRWYKVAKATADPGQPLYDELEDITTEPSTPAPEVIEIDHLT
                     QGGEGGLEVLVAYEPGGGAHATQRLVKWQIVGVDPGHIHSAPLDPSGNNLGAFAVGQT
                     IQVITEVSNSAGTRTSAPRSITLGEPVV"
     gene            3772..>4003
                     /locus_tag="PLP58_21360"
     CDS             3772..>4003
                     /locus_tag="PLP58_21360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ11144.1"
                     /translation="MSASTKRIFPVIPAGRKTETTHAPEQTMKIIQSLLTLLTVACMA
                     SCKSSSINLPLDHVKTSDGYLNSSSLDFGHVLV"
BASE COUNT          785 a         1373 c         1204 g          641 t
ORIGIN      
        1 gcgattcgaa ggcggcgcgc gcgtttgccc ccgccgcggc gcgcggctca ctcccacacg
       61 atgaggctgc gcgccacctc gccgcgctcc agcgcctcgt aggcctcgtt gatctgctcc
      121 agccgccagg tgcgggtgag cagctcgtcg agctgcagcg cgccgctctc gtgcaggcgc
      181 atgaggttca ccacgtctat gcgcgggcgc gtgctgccgt agagcgtgcc gcgcagcgtc
      241 ttctccgcat agacgagctg gttcacattg atggccgcgc gcgcgtcggc gcggcagatg
      301 ccggagacca cgcaggtgcc tccttttttc aggctgtcat acgcctgctc gatggcgcgc
      361 ggctggccga tggcctcaaa cgcgtagtcc acgccgcgcc cgccggtgag gtctttcacg
      421 gcctgcaccg cgtcgcactc cgcggcgttg atgaaatgcg cggcgcccat gcggcgcgcg
      481 tgcgcctctt ttccaggatg cgcgtccacc gcgatgaccg gcccggcgct gaccatgcgc
      541 gcgccctgga tgatgttcag cccgatgccg ccgcagccga acaccgccac gctgctgccc
      601 ggcggcactt tggcggcatg agtcaccgcg cccacgccgg tgatgacgcc gcaactgatg
      661 agcgccgcct tccacagcgg aaaagccgga tcaatgcgca ccaccgccgc ctccggcatc
      721 gtcgagagcg agctgaaggt ggacacgccc gcgtaatgcc ggatgctccg cccgcccgcg
      781 ctgcggaagc gcgtgttgcc gtcgggcatc agccccgtga agcgcatggc ggtccccatg
      841 tcgcacaaag ccgggcggcc tttgaggcag aaatcacagc gcccgcacga ggcgcgccag
      901 atgggcacca catggtcccc cggcgccacc gaggtgacac ccggccccgc ctcctccacc
      961 acgcccgcgc cctcgtggcc gaggatgatg ggcacgggca tgggcaggtc ccccttcatc
     1021 acatgcaggt cgctgtggca gacgcccgcc gccttcatgc gcaccatcac ctcccccgcc
     1081 tgcggcggca gcacctccac ctcctcgatg accatgggct gggcggactc atacaggacg
     1141 gcggcgggtg cggtgggcat ggcgtgcggc gggttggatc aaaaaaaccg ccccagactc
     1201 cccgcgcgcc ccgcctcccg caaccggaaa ccgcgcgcgc cgcgcatggc ttgcgccgga
     1261 gcgtgttgaa aatggacgcg cggtcgcgca tgagccgggc tttcagccct cacccggaga
     1321 ttgttcatgg tttgcccccg gttcccgggc cgctggcccg ggctggtata ctgaaggtca
     1381 tcaccttttg tgaattgtga tttcccgcag attcagggag gccggcagat ttctgctttt
     1441 catccttaat ctgtgggcct ccctgaatct gtgggagaaa accaaattca caaagcatga
     1501 tgacgttcag tgtagccgcc gccgcaaggc ggtgggtttg gaaaaagacg ccgcgctcct
     1561 acctttgcgc agcccgccac gggcttgccc ccgcagccac tagagacgcc gccgctgcta
     1621 gtagccgcca ccgcagggcg atggtccggc tgcgttattt ggcgtgttag gcgaggttcc
     1681 aagcacacgc catgccgcgc ggacgaagct caggccgccg gcctccagcg ccaaaggcgc
     1741 gcacgatacc agcccgggcc aacggcccgg gtgatgccgc ccgccgcacc aacccgatga
     1801 agggctgaaa gcccgcccca tgcacacgca atgcgcgctg cgccggagcc tgagctgaaa
     1861 atggacgtgt gccgcattgg gacaacgcgc ctgccccgcg acgggacggg gaaaagagct
     1921 gttcgcaacc cttcatgcag gcaaatccct gatgtcattc ttaatacgcc tgtcagcaac
     1981 agcaagactg ctttttgtca taatgcactg gctgtgaatg tcaatgcagc agctttttgc
     2041 atggctgcaa cggttgcagc tagcaaaaaa gcgaccgcaa gctattaaaa agcggctgca
     2101 attcatcaaa aagcgcctgc aacctataaa aaagcgccag catgaatgca aaaagctggc
     2161 gcagactggc tcatagcggc agcaaaaaca gccaaagtgc cttcagaggc attcatagcg
     2221 gctgcaggca gcttccagca ggtgcatggc tgatcagagc tgaaagttga tgtcaatcag
     2281 cgctcagagc gcaagccgca gcccgctcaa gggcttggga atttcttggt ggcgactctg
     2341 ccgctgaggc aggttttcca gacgcatcgg ccagcgcgca aggctggcat tttgcgccgc
     2401 gatgccgggc tttagccctg cttgaggcac agcagatgac tgtaacgcca ttgtcaagac
     2461 gcaccggatt agatcaaggt cttaggcgta ggattttccg agggaaaaat gcgttctgcg
     2521 gcagattttt ctcgacggaa ataaaaagat cgaatattcc gcgatttatg ccggaatctt
     2581 cctccagtgc gcgttgcgtg cgaagccttg caaaacaggc tgacggggat gttttcaccc
     2641 cgcatgggca tccggcgcgc caaacaaccg ccaacccgac caggccatgc caaatccagc
     2701 cggtgcccgc tatgatgacg gccttacata ttgggatgac ggctcaacct atgactccgc
     2761 cacagccgcg cagccgccgc ttttaactcc gcttcaaccc atctccccct caaccaccat
     2821 ggaattctgg gaagtcacca aaaaccgcgc gcaggaaacc ctgcccgtct gggccagcta
     2881 cgcgcccgac ctcaccgtcg gcggcaaaac ccccgccgac ctcgaggcgc tcattgacgg
     2941 cttcgagccg ctcgtgcagg cgcgcaccct ggcgcaggac ggcgtggacg cggcctatta
     3001 cgccatccag gacgcgctcc agcgcatgag agtgctgggc acgcgcgtgc cgcagatcat
     3061 cgaaggccag ctcgatgaaa acgcgctgct catgaaggac gtggacgacc tcttccgcac
     3121 cagcccgcgc accgagggca ccatcctcaa acgcctgcgc gacctgctgc ccgtgtggga
     3181 gcgcgcgaac acggcgctgg cggccatgac accgccgcag ccgcccgtca cgcgcaccgt
     3241 cggcggcgtg gcctacaccg tcgcgtcggc caccacgctg ctggacggct acaccgcgct
     3301 ggtcaaggcg cacaaggaca aggaggagga cctggacatc acccgccagg agctggccgc
     3361 gcacgaccgc gcgtgcgacc agctcaacaa gcgctggtac aaagtggcca aggccacggc
     3421 ggaccccggc cagccgctct acgacgagct ggaggacatc accaccgagc cgagcacgcc
     3481 ggcgccggag gtcatcgaga tagaccacct cacccagggc ggggagggcg gcctggaagt
     3541 gctcgtcgcc tacgagcccg gcggcggcgc gcacgccacg cagcgcctgg tgaagtggca
     3601 gatcgtcggc gtggaccccg gccacatcca cagcgccccc ctggacccca gcggcaacaa
     3661 cctcggcgcc ttcgccgtgg gccagacgat ccaggtcatc accgaagtca gcaacagcgc
     3721 cggcaccaga accagcgccc cgagaagcat cacgctcggg gagccggtgg tgtgagcgcc
     3781 tccacgaaac gaatttttcc ggtcatcccg gccggacgaa aaacagaaac aacacacgct
     3841 cctgaacaga ctatgaaaat tatacaatcg cttctaactc tgctgacggt cgcctgcatg
     3901 gcatcatgca agtcatccag cataaacctg cccctcgatc atgtgaagac atccgacggc
     3961 tatttgaatt catcctcgct ggattttgga catgtcctgg tct
//
LOCUS       DAOMYJ010000343         4003 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Prosthecobacter sp. isolate AMR_MDS_5629
            NODE_7998_length_4003_cov_15.257599, whole genome shotgun sequence.
ACCESSION   DAOMYJ010000343 DAOMYJ010000000
VERSION     DAOMYJ010000343.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867850
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prosthecobacter sp. (wastewater metagenome)
  ORGANISM  Prosthecobacter sp.
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Verrucomicrobiaceae; Prosthecobacter.
REFERENCE   1  (bases 1 to 4003)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4003)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.30
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:34:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,742
            CDSs (total)                      :: 4,684
            Genes (coding)                    :: 4,676
            CDSs (with protein)               :: 4,676
            Genes (RNA)                       :: 58
            tRNAs                             :: 55
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4003
                     /organism="Prosthecobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7998_length_4003_cov_15.257599"
                     /isolate="AMR_MDS_5629"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1965333"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            320..1549
                     /locus_tag="PLP58_21365"
     CDS             320..1549
                     /locus_tag="PLP58_21365"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009964392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HRJ11145.1"
                     /translation="MSLIVQKYGGTSVGNPERIRNCARRILETQRAGHKVVAVVSAMS
                     GVTDSLIKLAREVSPGGEPAEREMDVLLATGEQTTIALTAMAIQDLGGKAVSLTGAQA
                     GISTDRVHTKARIRNITPDAVHRMLDEGNIVMLAGFQGATDSGEITTLGRGGSDLTAI
                     AMAGAIKADLCQIFTDVDGVYTCDPRVVKAAAKIPEISYDEMLEMASSGSKVMQSRSV
                     EFAKKFGVRFEVRSSLNNNPGTLVKEETPGMESVVVRGVSLERNQAKVTIDDVPDRPG
                     ISAVIFGAIAQANVVVDMIVQNVAFDGETDISFTIAAADLPKAETALRAILPALGEKV
                     TLRTESGIAKLSVVGIGMRSHSGVAATMFRALADANANILMISTSEIKTAVIVREEEI
                     EKAARAVHTAFGLDAAA"
     gene            1600..2391
                     /locus_tag="PLP58_21370"
     CDS             1600..2391
                     /locus_tag="PLP58_21370"
                     /inference="COORDINATES: protein motif:HMM:NF018038.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ThuA domain-containing protein"
                     /protein_id="HRJ11146.1"
                     /translation="MIGSAMQPPIRVTIWNEFVHERQNAAVAEIYPRGIHAALAEGLG
                     GNTDFATRTATLDEPEQGLPPEVLDATDVLLWWGHAAHDAVLDETAARVQQRVLAGMG
                     LIVLHSGHFSKVFKRLMGTSCALCWREAGERERVWVVNPGHPIAAGLGACIEIGQSEM
                     YGEPFGIPAPDELVFVSWFQGGEVFRSGATWTRGSGRVFYFSPGHEVYPIYHHPEVLR
                     VIANGIRWARPQGAAADTPRHVPAEQARERVEPRGGSVAHPGQRT"
     gene            complement(2414..2647)
                     /locus_tag="PLP58_21375"
     CDS             complement(2414..2647)
                     /locus_tag="PLP58_21375"
                     /inference="COORDINATES: protein motif:HMM:NF014636.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoaD/ThiS family protein"
                     /protein_id="HRJ11147.1"
                     /translation="MLFFSLLQDITGCQELAVDDAPATVHALLEESIYPRWPALREWR
                     GSMLLALDHEYIRADAPLRPGFELALMPPVQGG"
     gene            complement(2668..3210)
                     /gene="hpt"
                     /locus_tag="PLP58_21380"
     CDS             complement(2668..3210)
                     /gene="hpt"
                     /locus_tag="PLP58_21380"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR01203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006166 - purine ribonucleoside salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HRJ11148.1"
                     /translation="MTSPTGLLSDLDRVLLSADAIRDRVREMAARIAADYQGSVVTVV
                     VLMDGALFFVADLLRQLDLPIRLVTLSASSYHGGTASTGEVRVQWPEAVEFAGQHVLL
                     LDDIYDTGLTLSALRARLLTERPASLRTGVLLAKKRPRAAEVPLDYRGFEIEDEFVVG
                     YGMDYQGRFRNLPCIGILKP"
     gene            3343..3972
                     /locus_tag="PLP58_21385"
     CDS             3343..3972
                     /locus_tag="PLP58_21385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009962742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HRJ11149.1"
                     /translation="MAGQPVISLLLVDDHFVVRSGLAASLELEDDMKVAGELGEAGMI
                     EQAYEKTRPDVVVMDLQLPGISGVEAAGRLLRAHPGARVLIFSTFARDEEIQAALKAG
                     ALGYVQKSASRGELLSAVRAVAAGRRHLPEGIEQRLRGLAAGPEITPRELDILRLVAR
                     GNANKEIAATLGIGEDTVKQHVSRILHKLDVNDRAQATAEAIRRGLVSV"
BASE COUNT          669 a         1269 c         1497 g          568 t
ORIGIN      
        1 ccccgggcca aacgggggcg accccgggcc aaatgggggc gaccccgggc caaatgggga
       61 cgccgccggg ccaaatgggg gtgccgccgg gcgaaatggg ggcgccccga aatgaaatgg
      121 gggcgccccc atttggcccg gcggggtcga aaaaggcggc aaagcggcgg tttcccaccc
      181 cgctggctcc acggcagacc acggcggctt gccagagccg gccgtcacgc tcccggcagc
      241 cggtttgcag ggagggccgg cgccatgctt ttccgttgcg cgcggcggcg ggcgtggcag
      301 tatcgcggcc cttttcccca tgtccctcat tgtccagaaa tacggcggca cctccgtcgg
      361 caacccggaa cgcatcagga actgcgcgcg gcgcatcctg gagacccagc gcgcgggcca
      421 caaggtggtg gcggtggtct cggccatgtc gggcgtgacc gacagcctga tcaagctggc
      481 gcgggaggtt tcccccggcg gggagcctgc cgagcgcgag atggatgtgc tgctggccac
      541 gggcgagcag acgaccatcg cgctgacggc gatggcgatc caggacctgg gcggcaaggc
      601 ggtctccctc accggcgcgc aggcgggcat ttccaccgac cgcgtccaca ccaaggcgcg
      661 catccgcaac atcacgccgg acgcggtcca ccggatgctg gacgagggga acatcgtcat
      721 gctggccggc ttccaggggg cgacggacag cggggagatc accacgctgg ggcgcggcgg
      781 cagcgacctg acggccatcg ccatggcggg ggccatcaag gcggacctgt gccagatatt
      841 caccgatgtg gacggcgtgt acacctgcga cccgcgcgtg gtgaaggcgg cggcgaagat
      901 ccccgagatc agctatgacg agatgctgga aatggccagc agcggcagca aggtgatgca
      961 gagccgcagc gtcgagtttg cgaaaaagtt tggcgtacgc tttgaggtgc gcagcagttt
     1021 gaacaacaac ccaggaaccc tcgtgaaaga agaaaccccc ggcatggaat ccgtcgtggt
     1081 gcgcggcgtc agcctcgagc gcaaccaggc caaagtcacc attgacgacg tgccggaccg
     1141 gcccggcatc tcggcggtca ttttcggcgc catcgcccag gcgaacgtgg tggtggacat
     1201 gatcgtgcaa aacgtggcct tcgatggcga gacggacatc tccttcacca tcgccgcggc
     1261 cgacctgcca aaggcggaga ccgccctgcg cgccatcctg cccgccttgg gggagaaggt
     1321 gacgctgcgc acggagagcg gcatcgccaa gctcagcgtg gtgggcatcg gcatgaggag
     1381 ccacagcggc gtggcggcca ccatgttccg cgcgctggcg gacgcgaacg cgaacatcct
     1441 catgatctcc acctcggaaa tcaagacggc ggtgatcgtc agggaggagg agatcgagaa
     1501 agcggcgcgc gcggtccaca cggccttcgg gctggacgcg gcggcctgag ggcgcgcgcc
     1561 gcagcgcgtg aaaatgcggc tttcatccgg cggcggcccg tgattggttc cgcgatgcag
     1621 ccgcccatcc gagtcacgat ctggaacgag tttgtccacg agcgccagaa tgccgccgtg
     1681 gcggagatct acccgcgcgg catccatgcg gcgctggcgg aggggctcgg aggaaacacg
     1741 gacttcgcca cccgcacggc gaccctggat gagccggagc aagggctgcc gccggaggtg
     1801 ctggacgcga cggatgtgct gctgtggtgg gggcacgcgg cgcacgacgc ggtgctggac
     1861 gagacggcgg cgcgcgtgca gcagcgggtg ctggcgggca tggggctgat cgtcctgcac
     1921 tcggggcact tttccaaggt cttcaagcgg ctcatgggca cgagctgcgc gctgtgctgg
     1981 cgcgaggccg gcgagcggga gcgcgtgtgg gtggtgaatc cggggcaccc catcgcggcg
     2041 gggctgggcg cgtgcatcga gatcgggcag tcggagatgt acggcgagcc tttcggcatc
     2101 cccgcgcccg acgagctggt gtttgtgtcc tggttccagg gtggcgaggt cttccgcagc
     2161 ggggccacct ggacacgcgg cagcggccgc gtcttttact tcagcccggg gcatgaggtg
     2221 tatcccatct atcatcaccc cgaggtgctg cgggtcatcg ccaacggcat ccgctgggcg
     2281 cgcccgcagg gggcggcggc ggacacgccg cgccatgtgc cggcggagca ggcgcgggag
     2341 cgcgtggagc cgcgcggagg cagcgtggcg catcccggac agcggacttg agcaggagtc
     2401 gtttttgaat tcctcaccct ccctgcaccg gcggcatgag ggcgagttcg aagcccggac
     2461 gcagcggcgc gtccgcgcgg atgtattcgt ggtcgagggc gaggagcatg gagccgcgcc
     2521 attcgcgcag cgccggccag cgcgggtaaa tgctttcctc cagcagggca tggacggtgg
     2581 ccggggcgtc atccacggcc agttcctggc agccggtgat gtcctggagc agggagaaaa
     2641 aaagcacgcg ccctttcatg agtccggtca gggtttgagg atgccgatgc agggcaggtt
     2701 gcggaagcgg ccctggtagt ccatgccgta gccgacgacg aactcgtcct cgatctcaaa
     2761 cccgcggtag tccagcggga cctcggcggc gcgggggcgc tttttggcca gcagcacacc
     2821 ggtgcgcagg ctggcgggcc gctccgtgag cagccgcgcg cgcagggcgg aaagggtcag
     2881 gccggtgtcg tagatgtcat ccagcaggag gacgtgctgc cctgcgaact cgaccgcctc
     2941 gggccactgc acccgcacct ctcccgtgga ggcggtgccg ccgtggtagc tgctggcact
     3001 gagggtgacg aggcggatgg gcagatcgag ctggcggagg aggtcggcca cgaaaaaaag
     3061 cgcgccatcc atgaggacca cgacggtgac cacgctgccc tggtagtccg cggcgatgcg
     3121 cgcggccatc tcgcggacgc ggtcgcgaat ggcatccgcg ctgagcagga cgcggtcgag
     3181 atcactcaaa aggccggtgg gtgaggtcat ggcccatcaa ggcgcaaagt gcgcgcggct
     3241 gcaaccggca gggctggcat tgaccgggat ttgacggcgg cgcgcgcgga tcagcggggc
     3301 ttttcacttg aagcgcacct ccggctttcc agtctggccc gcatggccgg ccagcccgtc
     3361 atctccctgc tgctcgtgga cgaccacttc gtcgtgcgca gcgggctggc ggcgtccctc
     3421 gaactggagg acgacatgaa agtggcgggc gagctgggcg aggccggcat gatcgagcag
     3481 gcctatgaaa aaacgcggcc cgatgtggtg gtcatggacc tgcaactgcc cggcatctcc
     3541 ggcgtggagg ccgccgggcg gctgctgcgc gcgcatcccg gcgcgcgggt tctcattttc
     3601 tccaccttcg cgcgggatga ggaaatccag gcggcgctga aggcgggcgc gctgggttat
     3661 gtgcagaaat cggcctcgcg cggggagctg ctctcggcgg tgcgcgcggt ggccgcgggg
     3721 cggaggcatc tgccggaggg catcgagcaa aggctgcgcg gcctggctgc cggacccgag
     3781 atcactccgc gcgagctgga catcctccgg ctcgtggcgc gcggcaacgc gaacaaggag
     3841 atcgccgcca cgctcggcat cggcgaggac accgtgaagc agcacgtcag ccgcatcctg
     3901 cacaagctgg atgtcaacga ccgcgcgcag gccacggcgg aggccatacg gcgcggcctg
     3961 gtcagcgtgt gaggcggcgc gcgcccctca cgctggcgcg gcg
//
LOCUS       DAOMYJ010000344         3984 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Prosthecobacter sp. isolate AMR_MDS_5629
            NODE_8066_length_3984_cov_19.039959, whole genome shotgun sequence.
ACCESSION   DAOMYJ010000344 DAOMYJ010000000
VERSION     DAOMYJ010000344.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867850
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prosthecobacter sp. (wastewater metagenome)
  ORGANISM  Prosthecobacter sp.
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Verrucomicrobiaceae; Prosthecobacter.
REFERENCE   1  (bases 1 to 3984)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3984)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.30
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:34:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,742
            CDSs (total)                      :: 4,684
            Genes (coding)                    :: 4,676
            CDSs (with protein)               :: 4,676
            Genes (RNA)                       :: 58
            tRNAs                             :: 55
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3984
                     /organism="Prosthecobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8066_length_3984_cov_19.039959"
                     /isolate="AMR_MDS_5629"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1965333"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..385
                     /locus_tag="PLP58_21390"
     CDS             <1..385
                     /locus_tag="PLP58_21390"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HRJ11150.1"
                     /translation="VGGGFTGLWTALRAAERDPAARILLIEGGAASSNNQPAGLLILV
                     TSGGWPVTDLNQDHFTVMQHFDTPGNTFSNNIVSFRNAGSGAYLIQVRPNGGAVWRSG
                     HLLGQLLVSSPDDRQGQAAFKLIIR"
     gene            complement(415..1053)
                     /locus_tag="PLP58_21395"
     CDS             complement(415..1053)
                     /locus_tag="PLP58_21395"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF016115.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016812 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in cyclic amides
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclase family protein"
                     /protein_id="HRJ11151.1"
                     /translation="MGTRKSRWKDITLPLRNGMAGWPGDAPFEITRDLKMEDGAVCNL
                     SRLSLSAHAGTHMDAPRHFIAQGAAMEEAPLDMLMGSCRVVLLDCADQITARDLEPLS
                     LRRGERGLFKTRNSRRDWSGEPFDKDFVSIRQDAAEHLAACGVALVGVDYLSIGGYEK
                     DGAATHQIILGAGIWVVEGLRLLDIHPGKYEMICLPLKIVGADGAPCRVLIR"
     gene            1201..2457
                     /locus_tag="PLP58_21400"
     CDS             1201..2457
                     /locus_tag="PLP58_21400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007417868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan 7-halogenase"
                     /protein_id="HRJ11152.1"
                     /translation="MNPETDVIIIGGGPSGAAAAAIIAEAGNKVLVLEREKFPRYHIG
                     ESLIPFTYAPLERLGMIPKMRGSCFTKKYSVSFVQPDGRRSVPFYFHTRYDINTVAQT
                     WQVTRAEFDVMLLDNAREKGAAVLEQTAVTRLLKNDSGTVIGVEAQGAGGVKRQFIAR
                     LVIDASGKEAFASNREGWRVGDPYLNKVAVWTYYKGSKREQGLDEGATTIAFIPDKGW
                     FWHIPMHDDRVSVGVVAEGKYLTRGGVRDPEAIFQREIRENKWIEEHLSQGRCEGEYW
                     LTSEYSRHSKYGAAPGLLLVGDAFAFLDPVFSSGVMLALKSGVMAGDAVNAGLAANDL
                     SPERFADYASQLRAGVENMRKLVYAFYDPDFSFKDVVTRHPDAAGEITDCLSGDVNKD
                     YSRLWERIREFCPLPGELPYGAPLHA"
     gene            2598..3620
                     /locus_tag="PLP58_21405"
     CDS             2598..3620
                     /locus_tag="PLP58_21405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018969771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamate-ammonia ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine synthetase beta-grasp
                     domain-containing protein"
                     /protein_id="HRJ11153.1"
                     /translation="MAKYKLEYIWLDGYTPVPNLRSKTQIKEYDSFPALEQLPMWGFD
                     GSSTQQAEGRSSDCMLKPVSVYPDSTRKNGVLVMCEVMMPDGVTPHPSNSRATILDDP
                     DAWFGFEQEYFLYQDGRPLGFPEGGYPAPQGPYYTGVGYKNVGDIARQIVEEHLDLCL
                     DAGINHEGINAEVAKGQWEFQIFGKGSKKCADQMWVARYILLRLCEKYCVDVEFHCKP
                     LGRDLDWNGSGMHANFSTKHLREVGGKEYFEKLMAAFAKNLNEHIAVYGPDNHMRLTG
                     LHETQSIDKFTYGIADRGSSVRVPHSFINNGYKGYLEDRRPNSQGDPYAIASRILKTI
                     AEVPLA"
     gene            3738..3811
                     /locus_tag="PLP58_21410"
     tRNA            3738..3811
                     /locus_tag="PLP58_21410"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3770..3772,aa:Gly,seq:ccc)
BASE COUNT          795 a         1384 c         1121 g          684 t
ORIGIN      
        1 cgtcggtggc ggcttcaccg gcctgtggac ggcgttgcgc gctgcggagc gcgaccccgc
       61 cgcgcggatc ctgctgatcg agggcggcgc ggcctccagc aacaaccagc cggccggcct
      121 tctcatcctg gtcacctccg gcggctggcc ggtgacggac ctcaaccagg accatttcac
      181 ggtgatgcag cacttcgaca ctccgggaaa cacgttcagc aacaacatcg tctccttccg
      241 caacgccggc tccggcgcct acctcatcca agtgcgcccc aacggcggag ccgtgtggcg
      301 ttccggccac ctgctcggcc agttgctggt ctcctctccc gatgaccgcc agggccaggc
      361 cgccttcaag ctcatcatcc gctgaggcgc tccgcgcgcg gggccgccag gggatcagcg
      421 gatgagcacg cggcaggggg cgccgtccgc tcccacgatt ttcagcggca ggcagatcat
      481 ctcgtacttg ccgggatgga tgtccagaag ccgcagcccc tccaccaccc agatgcccgc
      541 gccgagaatg atctgatggg tggccgcgcc gtccttttca tatcctccga tgctgaggta
      601 atccaccccg actaatgcga ccccgcacgc ggccagatgc tcggcggcat cctggcggat
      661 ggagacgaaa tccttgtcaa aaggctcccc ggaccagtca cggcgggaat tccgcgtctt
      721 gaacagcccc ctctcgcccc gccgcaatga caaaggctcc agatcccgcg ccgtgatttg
      781 atccgcgcag tccagcagga cgacacggca ggagcccatg agcatatcca ggggcgcctc
      841 ctccatggcg gccccttgcg cgatgaaatg gcgcggcgcg tccatgtgcg tgcccgcatg
      901 ggcgctgagg gacaggcggg agagattgca cacggccccg tcctccattt tcaaatcgcg
      961 cgtgatctca aaaggcgcgt cgcccggcca gccggccatg ccgttgcgca ggggtagggt
     1021 gatgtctttc cagcgtgatt tccgggtgcc cataaacctc gcggcataaa cgcgactgcc
     1081 aatgcggtca atgatctcat gccggcggca agctccatgc gcaccgatgc cgtcccggca
     1141 tcatcgccat gctgcaatag aatctcgcgc ggccagcgcc gccgcgtatc aatgcatggc
     1201 atgaatccag aaacagacgt catcatcatc ggcgggggcc cctcgggcgc cgccgccgcc
     1261 gccatcatcg cggaggccgg caacaaagtg ctcgtcctgg agcgggaaaa atttccccgc
     1321 taccacatcg gcgagtcatt gatccccttc acttacgctc cgctggaacg ccttggcatg
     1381 atccccaaaa tgcgcggctc ctgcttcacc aaaaaataca gcgtcagctt tgtgcagccc
     1441 gacggacggc gctccgtgcc cttttatttc cacacccgtt acgacatcaa caccgtggct
     1501 cagacctggc aggtcacgcg cgcggagttc gatgtcatgc tgctcgacaa cgctcgtgaa
     1561 aaaggcgccg ccgtcctgga gcagaccgcc gtcacgcgcc tgctgaaaaa cgacagcgga
     1621 acagtgattg gagtcgaggc ccagggcgcc ggcggtgtga aacgccagtt tatcgcccgc
     1681 cttgtcatag acgcgtccgg caaggaagcc ttcgcctcca accgggaggg ctggcgcgtc
     1741 ggcgacccgt atctgaacaa ggtcgccgtc tggacctact acaaaggctc caagcgggag
     1801 caaggcctgg acgaaggcgc caccaccatc gccttcatcc ccgacaaagg ctggttctgg
     1861 cacatcccca tgcatgacga ccgcgtcagc gtgggcgtcg tggccgaggg caaatacctc
     1921 acgcgcggcg gcgtgcgcga tccggaggcc atcttccagc gggaaatccg ggagaacaaa
     1981 tggatcgagg agcacttgtc ccagggccgc tgcgaaggcg aatactggct caccagcgag
     2041 tattcccgcc actccaaata cggcgccgcc cccggcctgc tgcttgtcgg ggacgccttt
     2101 gcctttctcg accccgtctt cagcagcgga gtcatgcttg ccctgaaaag cggcgtcatg
     2161 gccggggacg ccgtcaacgc agggctggcc gccaacgacc tctcaccgga acgcttcgcc
     2221 gactatgcca gccagctccg cgccggcgtg gagaacatgc gcaagctcgt ctatgccttt
     2281 tacgaccccg atttctcctt caaggatgtc gtcacccgcc atcctgacgc cgccggcgag
     2341 atcaccgact gcctttccgg cgatgtgaac aaggactact cacgcctctg ggagcgcatt
     2401 cgcgagttct gcccgctgcc cggagagctg ccctacggcg cgcccctgca cgcctgagca
     2461 ggcggccgga ttgaagcgcc ggaggcaaaa acccccggaa aaaaacattt cacttctggc
     2521 acgaggcgtg ctttttctgc acgcgctgac cggcgcgcat cataagccgg catcaatccg
     2581 aacctcgaac aacaaccatg gcaaaataca aactcgaata catttggctc gacggttaca
     2641 cccccgtgcc aaacctgcgc agcaagacgc aaatcaagga atacgacagc ttccccgcgc
     2701 ttgagcagct tcccatgtgg ggcttcgacg gcagctccac ccagcaggct gaaggccgca
     2761 gctccgactg catgctcaag cccgtctccg tctatcctga cagcacccgc aaaaacggcg
     2821 tgctcgtcat gtgcgaggtc atgatgcccg acggcgtcac cccgcacccc tccaacagcc
     2881 gcgccaccat ccttgacgat cctgacgcat ggtttggctt cgagcaggaa tacttcctct
     2941 accaggacgg acgcccgctc ggcttccccg aaggtggcta ccccgcccct caaggcccct
     3001 actacaccgg agtcggttac aaaaacgtcg gcgacatcgc ccgccagatc gtcgaggagc
     3061 acctcgacct ctgccttgac gccggcatca accacgaagg catcaacgcc gaggtggcca
     3121 aaggccagtg ggaattccaa atcttcggca aaggctccaa aaagtgcgcc gaccagatgt
     3181 gggtggcccg ctacatcctc cttcgcctct gtgagaaata ttgcgtggat gtcgaattcc
     3241 actgcaagcc tctcggcagg gatctcgact ggaacggctc cggcatgcac gccaacttct
     3301 ccaccaagca cctgcgcgaa gtcggcggca aggaatactt cgagaagctc atggccgcgt
     3361 tcgccaaaaa cctcaacgag cacatcgccg tttacggacc cgacaatcac atgcgcctca
     3421 ccggcctgca tgagacccag tccattgaca agttcaccta cggcattgcc gaccgcggct
     3481 cctcggtccg cgtcccgcac agcttcatca acaacggtta caagggctac cttgaggacc
     3541 gccgccccaa ctcccagggc gacccctatg ccatcgcctc ccgcatcctc aaaacgatcg
     3601 cggaagtgcc gctggcctga gttttgccca cggaacctcg ctctgacgcc gcctggagac
     3661 aggcggcgtt tttgttttga tgaatccggg cgcgccttga catcgcatgc cgaagcgcca
     3721 gtttccaacc actcgttgcg ggtgtagttc aatggtagaa cacgagcttc ccaagcttga
     3781 tacgtgggtt cgattcccat cacccgctcc acgcaaaatt aagggttcca cagcgtgtgc
     3841 agtaaaagtg cagcggggga gaggcgcact ttgcccatct gcccacggtg aacctcggct
     3901 gcgacgcgct gcatctcaaa gaactcgctg cgagcgatgc tgaggcagtg cgtgtcgtca
     3961 tccgcgacaa cgccgggttc catc
//
LOCUS       DAOMYJ010000345         3942 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Prosthecobacter sp. isolate AMR_MDS_5629
            NODE_8172_length_3942_cov_16.780036, whole genome shotgun sequence.
ACCESSION   DAOMYJ010000345 DAOMYJ010000000
VERSION     DAOMYJ010000345.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867850
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prosthecobacter sp. (wastewater metagenome)
  ORGANISM  Prosthecobacter sp.
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Verrucomicrobiaceae; Prosthecobacter.
REFERENCE   1  (bases 1 to 3942)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3942)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.30
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:34:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,742
            CDSs (total)                      :: 4,684
            Genes (coding)                    :: 4,676
            CDSs (with protein)               :: 4,676
            Genes (RNA)                       :: 58
            tRNAs                             :: 55
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3942
                     /organism="Prosthecobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8172_length_3942_cov_16.780036"
                     /isolate="AMR_MDS_5629"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1965333"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            195..1085
                     /locus_tag="PLP58_21415"
     CDS             195..1085
                     /locus_tag="PLP58_21415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009965774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase"
                     /protein_id="HRJ11154.1"
                     /translation="MTEFIERFIQHLAVERGLSLNYQLLVRRVLESMASWFQQEKNLA
                     DPAGVTTAHLADLLARRKKDGLAASSARVELVAVKIFFRWLAQRGHIPRDPAESILPP
                     RLEKHLPGTLNEEHARRLVESITGAGPLDRRDRAMLELFYASGLRLSELLESRLENLS
                     LDEGWIRVTGKGGKTRLAPVGQAAREALAGWLQHGRPALVGPKTGSHVFLNRRGGTLT
                     RARVWQIVKQRAAHAGLEQRVHPHLLRHSFATHLLNNGADLRVIQEMLGHADISTTQI
                     YTHVDQKRLKQVHRKFHPRG"
     gene            complement(1151..2515)
                     /locus_tag="PLP58_21420"
     CDS             complement(1151..2515)
                     /locus_tag="PLP58_21420"
                     /inference="COORDINATES: protein motif:HMM:NF025885.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HRJ11155.1"
                     /translation="MAKLVIIDDETAILELMSKLCRAAGHTVFGCTTGLDGINAIRTQ
                     KPELVIVDLRIGDVNGLDLVSMCHEQFPDTAVIMVTGHGSVETAVEAMRLGAFDYLTK
                     PFDLGDLIKTVNQALNRNNPGAQAAAMPVTTSVRSPGASKLIGHSGAVQRIFEILRRV
                     ADNDSPVLLEGEFGVGKHMVARTLHDSSRRASAPFKELQCSAMPEDALEAELFGHANG
                     AQGIFGRATGGTVHLAEVHVLPARIQAQLNTFLDETQARRSAWSNGGSHFRLVSSTTV
                     SLEEEAKEGRFREDLFYKLSVVPLKIPPLRERREDIKPLVDHFLKELSDRMDGRPFSM
                     DSYAFEFLEKYSWPGNISELRNAVERACAFAENYRVKPADLPAKVTQQIEVPTTNASN
                     GVTQLPIGSTLDDFIRGQERLFIQETLKYNNGSREKTASMLGVSIATLYRKMGLNVDR
                     KTTA"
     gene            complement(2705..>3942)
                     /locus_tag="PLP58_21425"
     CDS             complement(2705..>3942)
                     /locus_tag="PLP58_21425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ11156.1"
                     /translation="ARADLRLRRAAGIVTGLALLHALFDVPLHRPAYFMVAAVTAALA
                     LRPERLATATGRAGRFFFRSAGTAVLGLGAVYLGLHLGVWMPPSGTTAGVLHDRAVAE
                     SAAGRHSAALADMEKAVTLAPLNFRMYFLRAQLQLKLRQPAEQALMDFSRARALEPGH
                     AGMCFDEGLYWLGFRPLFAIIPWRECLRRDPEAAQGIHGFYQRMLNSMGQHPELAEPL
                     WQLASQVELQILYLRSAPGGEMWQARLDKVLAANPRLRMTDKAGLRMIFDAWALKGDS
                     TKLEAGLRQHPEWQTYGWRHLAAAEAREGRFREACDLAAAHLPASARSTSLGVADIPR
                     LERVVLLNPEDARPAVELYYAQRASGDYKAALRNLEKIATLPNPPDFLQREMAAMHMQ
                     LEDYQRAWEILRGIIGASS"
BASE COUNT          667 a         1425 c         1178 g          672 t
ORIGIN      
        1 tgctgcaggt gctcgaaggc atgaccgcgc ccaccccggc agccttcgcc gcctccgcca
       61 gacgcgtcct ctccgagcgc gtccgcaact cgccgcccgc gcgcaacgag cagcgctggc
      121 tcgcaggctg gcacaaccgt tgcgatggct acctgcgccc tttgtgatca cgccagccgc
      181 cgcgcgccca cgtcatgacc gagttcatcg agcgcttcat ccagcacctc gccgtcgagc
      241 gcggcctctc cctgaattac cagctcctgg tgcgccgcgt gctcgagtcc atggcatcct
      301 ggttccagca ggaaaaaaac ctcgccgacc ccgccggcgt caccaccgcc catctcgccg
      361 acctcctcgc ccggcgcaaa aaggacggcc tcgccgcctc aagcgcccgc gtcgaactcg
      421 tcgccgtcaa aatcttcttc cgctggctcg cccagcgcgg ccacatcccg cgcgaccccg
      481 ccgaatccat cctgccgccg cgcctggaga agcacctgcc aggcacgctc aatgaggagc
      541 acgcccgccg cctcgtcgag tccatcaccg gcgcgggccc gctcgaccgg cgcgaccgcg
      601 ccatgctcga gcttttctac gccagcggcc tgcgcctcag cgagctgctc gaatcccgcc
      661 tggaaaatct cagcctcgat gaaggctgga tccgcgtcac cggcaaaggc ggcaaaaccc
      721 gcctcgcacc cgttggacaa gccgcgcgcg aggccctggc cggctggctc cagcacggta
      781 ggcctgccct ggtcggaccg aaaacgggca gccatgtctt tttaaaccgg cgcggcggca
      841 cgctcacgcg cgcccgcgtc tggcagatcg tcaaacagcg cgccgctcat gccggactgg
      901 agcagcgcgt gcatccgcac ctgctgcgcc acagtttcgc cacccacctg ctcaacaacg
      961 gcgccgacct ccgcgtcatc caggaaatgc tcggccacgc cgacatcagc accacccaga
     1021 tttacacgca tgtggaccag aagcgcctca agcaggtcca ccgcaaattc cacccgcgcg
     1081 gttgacgcgc cctgcccttc ccgcgcggca aggcctgtca taggttcgct gcgggtgacg
     1141 gcgcgcacgc ctacgccgtg gtcttgcggt ccacgttgag tcccattttg cggtagagcg
     1201 tggcgatgct gactccaagc atcgaggccg tcttctcgcg ggagccgttg ttgtacttca
     1261 gcgtctcctg gatgaacagg cgctcctgcc cgcggatgaa gtcatccagt gtggagccga
     1321 tggggagctg ggtgacgccg ttgctggcgt tggtggtcgg cacctcgatc tgctgggtga
     1381 ccttcgcggg caggtcggcg ggcttgacgc ggtagttttc cgcgaaagcg caggcgcgct
     1441 cgacggcgtt gcgcagctcg gagatgttgc cgggccagga atacttctcc aggaactcaa
     1501 aggcgtagct gtccatggag aaaggccgcc cgtccatgcg gtcggagagc tccttgagga
     1561 agtggtccac cagcggtttg atgtcctcgc ggcgctcgcg cagcggcggg atcttcaaag
     1621 gcacgaccga gagcttgtaa aaaagatcct cgcggaagcg gccctccttg gcctcctcct
     1681 ccagcgagac ggtggtggag ctgacaaggc ggaaatggct gccgccgttg ctccaggcgc
     1741 tgcggcgggc ttgtgtctcg tccaggaaag tgttcaactg cgcctggatg cgcgcgggca
     1801 gcacatgcac ctcggccaga tgcacggtgc cgccggtggc gcggccgaag atgccctgcg
     1861 cgccgttggc gtggccgaag agctcggcct ccagcgcgtc ctccggcatg gcgctgcatt
     1921 gcagctcctt gaagggcgcc gacgcgcgcc ggctcgagtc atgcagcgtg cgcgccacca
     1981 tgtgcttgcc cacgccaaac tcgccctcca gcagcaccgg gctgtcattg tccgccaccc
     2041 ggcgcagaat ctcgaaaatg cgctgcaccg ctccgctgtg gccgatcagc ttcgaggcgc
     2101 cggggctgcg cacactggtc gtgaccggca tggccgcggc ctgggcgccg ggattgttgc
     2161 ggttcagcgc ctggttgacc gtcttgatca gatcccccag atcgaagggc ttcgtcaggt
     2221 agtcaaacgc gcccagccgc atcgcttcca ccgccgtctc cacgctgccg tggcctgtca
     2281 ccatgatgac cgccgtgtcg gggaactgtt cgtggcacat gctcaccaga tcgaggccgt
     2341 tcacatcgcc aatgcgcaga tccacaatga caagctcggg cttctgggtg cggatcgcat
     2401 tgatgccatc cagacccgtc gtgcagccaa acacggtgtg gccggctgcc cggcagagct
     2461 tgctcatgag ctccaggatg gcggtctcgt catcaatgat tactagtttg gccataagaa
     2521 attagaattt ttggaatcaa agctgattat caagaatgat aaattaattc tcacgctcgt
     2581 caatcatgtt tctttttttc attcattcct gctgtctgcc ttttaggttt gcgtgtccgc
     2641 gctcatgccg cgtcttatct tccgcaggac tcttcagcgg aacatgaatg agactttcat
     2701 gccgctacga ggatgctccg atgattccgc gcaaaatctc ccacgcgcgc tggtagtcct
     2761 caagttgcat gtgcatggcc gccatttcac gctggagaaa gtcgggcgga ttcggaagcg
     2821 tggcgatttt ttccaggttc cgcagcgccg ccttgtaatc accagacgcc cgttgcgcgt
     2881 aataaagctc caccgccggc ctggcatcct ccggattcag cagcaccacc cgctccaggc
     2941 gcgggatgtc cgccaccccc aggctcgtgc tgcgcgcgga cgcgggcaga tgcgcggcgg
     3001 ccaggtcgca cgcctcgcgg aagcgcccct cgcgcgcctc ggcggcggcc agatgccgcc
     3061 agccgtacgt ctgccattcg gggtgctggc gcaggcccgc ctcgagcttg gtgctgtcgc
     3121 ccttcagcgc ccaggcgtcg aagatcatcc tcagccccgc cttgtccgtc atgcgcaggc
     3181 gcggattggc cgccagcacc ttgtccagcc tggcctgcca catctccccg cccggagccg
     3241 agcgcaggta aagaatctgc agctccacct ggctggcgag ctgccacagc ggctccgcca
     3301 gctcgggatg ctggcccatc gagttcagca tccgctggta aaagccgtga atgccctgcg
     3361 ccgcctccgg atcacggcgc aggcactcgc gccatgggat gatggcaaag agcggcctga
     3421 aacccagcca gtaaagccct tcgtcaaagc acatccccgc gtggcccggc tccagagcgc
     3481 gcgcccggct gaaatccatc agcgcctgtt ccgcgggctg gcgcagcttt agttggagct
     3541 gcgcgcgcag gaagtacatg cggaaattca aaggcgccag cgtcaccgcc ttctccatgt
     3601 cggccagcgc cgcgctgtgc cgccccgctg ccgactccgc caccgccctg tcatgcaaaa
     3661 cccccgccgt cgtccccgag ggcggcatcc acacacccag gtgcagcccc agatacaccg
     3721 cccccagtcc cagcaccgcc gtccccgccg agcggaaaaa aaaccgcccc gcgcgcccgg
     3781 tcgccgtcgc caagcgctcc ggtctcaaag ccagcgccgc cgtcaccgcc gccaccatga
     3841 aataagccgg gcggtgcagc ggcacatcga acagcgcatg cagcagcgcc agccccgtca
     3901 cgatccccgc cgcgcgccgc aggcgcaggt cggcccgcgc cc
//
LOCUS       DAOMYJ010000346         3892 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Prosthecobacter sp. isolate AMR_MDS_5629
            NODE_8313_length_3892_cov_17.037269, whole genome shotgun sequence.
ACCESSION   DAOMYJ010000346 DAOMYJ010000000
VERSION     DAOMYJ010000346.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867850
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prosthecobacter sp. (wastewater metagenome)
  ORGANISM  Prosthecobacter sp.
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Verrucomicrobiaceae; Prosthecobacter.
REFERENCE   1  (bases 1 to 3892)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3892)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.30
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:34:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,742
            CDSs (total)                      :: 4,684
            Genes (coding)                    :: 4,676
            CDSs (with protein)               :: 4,676
            Genes (RNA)                       :: 58
            tRNAs                             :: 55
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3892
                     /organism="Prosthecobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8313_length_3892_cov_17.037269"
                     /isolate="AMR_MDS_5629"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1965333"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..285
                     /locus_tag="PLP58_21430"
     CDS             <1..285
                     /locus_tag="PLP58_21430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ11157.1"
                     /translation="DEADNVALNLRQYGNRFGGNPAGTNAEIVKALNGGNPQGVRFLP
                     PELLRLNAQGELVDRWDTPYFFHQMSADHMEVRSAGPDKTLWTSDDIVTR"
     gene            complement(391..2907)
                     /locus_tag="PLP58_21435"
     CDS             complement(391..2907)
                     /locus_tag="PLP58_21435"
                     /inference="COORDINATES: protein motif:HMM:NF019607.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-dependent sugar dehydrogenase"
                     /protein_id="HRJ11158.1"
                     /translation="MTTTRHLSLPAALLALLACHAAHAAFPTVALRPVVLQQIHSPTV
                     ITHAPDGSGRLFVCDQHGKIFIIRNGMMLPAPFLNLASPANTLIEDRGPGPVITLGTG
                     YSERGLLGLAFHPGFADPGSPGYRKFYVNYIKADADGEPDPPQAGDPVNCITVIDEFE
                     VSAADPDLADPASQRRVLAFTQPQANHNGGALEFGPDSMLYIGSGDGGSSNDNAAGHT
                     GREVSTTACLGNSQDKTRYLGKILRINPLDPDGGGPLTYSIPADNPFFSDATPGLKIE
                     IYALGLRNPWKFSFDWRPGGTDRLFCGDVGQGRIEEINLIVSGGNYGWRYQEGLELPT
                     FSSGAGSNPMPHPGGVLITPIAMYAHPGVNTSPPLPQLGLSVTGGHVYRGQAIPEMQG
                     KYVFGDYGSTGGASDGRLMGLEETAPGSGVFTLTQALPLFGQANPVTGERILCLGEDE
                     AGEIYIGMKSNAGVLARIGGLPAGGIYQIVPVETVTTVLNPQLDNTIYSEDGGLSDAR
                     GYFYAGRTGPNHGPYLRRALLSFNVEIIPPEAALVSASLKLRLNKLGPGGSGTPMALH
                     RLNQTWGEGTSKNPSDVGTGAPATPGDATWSHRFHDTSTWTTPGGVFEAAPSAAATAD
                     VGTITFGPSAQMLADAQAWHADPAANAGWMLRGDETQNETVGRFDSKEVGQVFPELEI
                     QYQLPPPVPPFGTWLKGKFPALLTGQFVDIFDDQEGDSIPLLLEYALGFDPFTRDTDS
                     GLHLSVAPGEEGGTDLTLTFRRETAATDLVLRLEMSASPVAWGTPLAEVSAGQIQTMN
                     GADLIADDWLVDTTNLVTLRLNLPETPGQRRFVRMSVDWQ"
     gene            complement(2935..>3892)
                     /locus_tag="PLP58_21440"
     CDS             complement(2935..>3892)
                     /locus_tag="PLP58_21440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018969015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="cytochrome c peroxidase"
                     /protein_id="HRJ11159.1"
                     /translation="MGAAFPQLALPADNPLTAERSGLGKRLFHETALSRDGSISCASC
                     HHAGAALSDPRRFSPGIEGRTGDRNAMPLFNLAWKKEFFWDGRAPSLRAQALIPIEDH
                     REMDEKLENVVAKLERQPAWTAAFQKAFGTPEITPERIGLALENFLLGLTSHDSKFDK
                     AQRGEAQLSAEEQRGFELFMTEREPRLGALGADCFHCHGGALFTDHQFRNNGLPVSDA
                     DPGRYKVTGVSIDRGAFATPSLRNIALTAPYMHDGRFATLEEVVDHYSTGVQRTDTLD
                     PNLAKHPDGGLHLTQEDKKALLAFLRTLTDDRFAASAHADPP"
BASE COUNT          558 a         1075 c         1589 g          670 t
ORIGIN      
        1 gacgaggcgg acaacgtcgc gctgaacctc cgccaatacg gcaaccgctt cggaggcaac
       61 cccgccggga ccaatgcgga gatcgtcaag gcactcaacg gcggcaaccc ccagggcgtg
      121 cgtttcctgc cgccggagct gctgcgcctc aacgcgcagg gagagctggt ggaccgctgg
      181 gacaccccct atttcttcca ccaaatgtcc gccgaccaca tggaggtgcg ctccgccggt
      241 ccggacaaga cgctgtggac ctcggatgac atcgtgaccc gctgatccgg gtggtttcgg
      301 caggcaggtt gaccgggcga aagaactcgg cgggcgctgt gccaggcgag ttttgacttg
      361 gctggggctg cttttccgca gggttgggct ttactgccag tccacgctca tgcgcacgaa
      421 gcggcgctga ccgggggttt cgggcaggtt caagcgcagg gtgacgaggt tggtggtgtc
      481 cacgagccag tcgtcggcga tgaggtcggc gccgttcatg gtctgaatct ggccggcgct
      541 gacttcggcg aggggggtgc cccaggctac cggactggcg ctcatttcca gacgaaggac
      601 gaggtcggtg gcggcggtct cgcggcggaa ggtgagggtg aggtcggtgc cgccctcctc
      661 gccgggggcc acgctgaggt ggagaccgct gtccgtgtcg cgggtgaagg gatcaaaacc
      721 gagggcgtat tcgagaagga gggggatgga gtcgccttcc tggtcgtcga agatgtccac
      781 gaactggccg gtgagaaggg cggggaattt gcctttgagc caggttccga agggcggcac
      841 cgggggcggg agctggtatt ggatttcgag ttcggggaag acctggccga cttccttgct
      901 gtcgaagcgg cccacggtct cgttttgcgt ctcatccccg cgcagcatcc agccggcgtt
      961 ggcggctggg tcggcgtgcc aggcctgcgc gtcggccagc atctgggcgc tggggccgaa
     1021 ggtgatggtg ccgacatcgg cggtggcggc ggcggagggc gcggcctcga agacgccgcc
     1081 tggggtggtc caggtgctgg tgtcgtggaa gcggtggctc caggtggcgt cgccgggggt
     1141 ggcgggcgcg ccggtgccca cgtcggaggg gtttttggag gtgccctcgc cccaggtctg
     1201 gttcaggcgg tgcagggcca tgggagtgcc ggagccgccg gggccgagct tgttgaggcg
     1261 gagcttgagg ctggcggaga cgagcgcggc ctcgggcggg atgatctcga cattgaagga
     1321 aagcagggcg cggcgcaggt aggggccgtg attggggccg gtgcggccgg cgtaaaaata
     1381 gccgcgcgcg tcgctgaggc cgccgtcctc ggagtagatg gtgttgtcca actgcgggtt
     1441 gagcacggtg gtgactgtct cgacggggac gatctggtag atgccgccgg caggcaggcc
     1501 gccgatgcgc gccagcacgc cggcattgct tttcatgccg atgtagattt cccccgcctc
     1561 gtcctcgccg aggcagagga tgcgctcgcc ggtgacgggg ttcgcctggc cgaagagcgg
     1621 cagcgcctgg gtgagggtga agacgccgct gccgggcgcg gtctcctcca gacccatgag
     1681 ccgcccatcg gaggcgccgc cggtggagcc gtagtcgccg aagacgtatt tgccctgcat
     1741 ttcgggaatg gcctggccgc gatagacgtg gccgccggtg acggaaaggc cgagctgggg
     1801 caggggcggg ctggtgttga cgccggggtg ggcgtacatg gcgatggggg tgatcaaaac
     1861 gccgccaggg tggggcatgg ggttgcttcc cgcgccggag gagaaggtgg gcagttccag
     1921 cccctcctgg taacgccagc cgtagttgcc gccggagacg atgagattga tctcctcgat
     1981 gcggccctgg ccgacatcgc cgcagaagag ccggtccgtg ccgccgggcc gccagtcgaa
     2041 ggagaacttc cacggattgc gcaggccgag ggcgtagatt tctattttca ggccgggagt
     2101 ggcgtcgctg aagaaggggt tgtcggcggg gatggagtaa gtgagcgggc cgccgccgtc
     2161 ggggtcgagg gggttgatgc ggaggatctt gccgaggtag cgtgtcttgt cctggctgtt
     2221 gccgaggcag gcggtggtgg agacctcgcg cccggtgtgg cccgcggcgt tgtcattgct
     2281 gctgccgccg tcgccgctgc cgatgtagag catgctgtcg gggccgaact cgagcgcgcc
     2341 gccgttgtgg ttggcctgcg gctgggtgaa ggcgagcacg cggcgctggg aggcggggtc
     2401 ggccaggtcc gggtcggcgg cggagacttc gaactcgtca atgacggtga tgcaattgac
     2461 ggggtcgcct gcctgtggcg ggtcgggttc gccatcggcg tcggctttga tgtagttcac
     2521 ataaaacttg cggtagccgg gcgaccccgg atcggcgaag ccggggtgaa aggccaggcc
     2581 gagcaggccg cgctcgctgt agccggtgcc gagggtgatc acggggccgg ggccgcggtc
     2641 ttcgatcagc gtgttggcgg ggctggcgag gttgaggaag ggcgcgggca gcatcatgcc
     2701 gttcctgatg atgaagatct tgccatgctg gtcgcagacg aagaggcgtc cgctgccgtc
     2761 gggggcgtgg gtgatgacgg tgggagagtg gatctgctgg agcaccaccg gcctgagcgc
     2821 gacggtgggg aaggcggcgt gtgcggcgtg gcaggccagc agcgcgagga gcgcggcagg
     2881 caggctgagg tggcgggtgg ttgtcatggc tggcgggtcc gggaggctgc ccggtcaggg
     2941 tgggtcggca tgggcggagg cggcgaagcg gtcgtcggta agggtgcgca ggaaggcgag
     3001 cagggctttt ttgtcctcct gggtgaggtg caggccgccg tcggggtgct tggccaggtt
     3061 ggggtcgagt gtgtcggtgc gctggacgcc ggtgctgtag tggtcgacca cttcctcaag
     3121 cgtggcgaag cgcccgtcgt gcatgtaggg ggcggtgagg gcgatgttgc gcaggctggg
     3181 cgtggcgaag gcgccgcggt caatggagac gccggtgact ttgtagcggc cggggtcggc
     3241 atcggagacg ggcaggccgt tgttgcggaa ctggtggtcg gtgaaaagcg cgccgccgtg
     3301 gcagtggaag cagtccgcgc cgagggcgcc gaggcgcggc tcgcgctcgg tcatgaacag
     3361 ctcgaagccg cgctgctcct cggcgctgag ctgcgcctcg ccgcgctggg ctttgtcgaa
     3421 cttcgagtcg tgggaggtga ggccgagcag gaagttttcc agggccaggc cgatcctttc
     3481 cggggtgatc tccggcgtgc cgaaggcctt ttgaaacgcc gccgtccagg cgggctggcg
     3541 ctccagtttg gcgacgacgt tttcgagctt ttcatccatc tcgcggtggt cctcgatggg
     3601 gatgagcgcc tgcgcgcgca ggctgggggc gcggccatcc cagaagaact cctttttcca
     3661 cgccaggttg aaaagcggca tggcgttgcg gtcgccggtg cggccttcga tgccggggct
     3721 gaagcgccgg ggatcactca aggcggcgcc ggcgtggtgg caggaggcgc aggagatgga
     3781 gccgtccctg gacagagcgg tttcgtggaa gagccgttta ccaaggccgg agcgctccgc
     3841 cgtgaggggg ttgtccgccg gcagcgcgag ctgcgggaag gcggcgccca tg
//
LOCUS       DAOMYJ010000347         3883 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Prosthecobacter sp. isolate AMR_MDS_5629
            NODE_8336_length_3883_cov_21.143156, whole genome shotgun sequence.
ACCESSION   DAOMYJ010000347 DAOMYJ010000000
VERSION     DAOMYJ010000347.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867850
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prosthecobacter sp. (wastewater metagenome)
  ORGANISM  Prosthecobacter sp.
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Verrucomicrobiaceae; Prosthecobacter.
REFERENCE   1  (bases 1 to 3883)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3883)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.30
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:34:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,742
            CDSs (total)                      :: 4,684
            Genes (coding)                    :: 4,676
            CDSs (with protein)               :: 4,676
            Genes (RNA)                       :: 58
            tRNAs                             :: 55
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3883
                     /organism="Prosthecobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8336_length_3883_cov_21.143156"
                     /isolate="AMR_MDS_5629"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1965333"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..361
                     /locus_tag="PLP58_21445"
     CDS             <1..361
                     /locus_tag="PLP58_21445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ11160.1"
                     /translation="LRGTGGRVSTLGSATAPDVLQSHLAADRAVLVSMPVPDAGGRSW
                     ARLLGVNDSTVRVTDPATGDPVSYPRSVFDQVTRGQDGYFILDPWRDTEGAPRTEPAP
                     VPRVLADLDELLTPAVE"
     gene            365..811
                     /locus_tag="PLP58_21450"
     CDS             365..811
                     /locus_tag="PLP58_21450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ11161.1"
                     /translation="MNLSPGSIHFPPQRHYRVTRRLPLLAVCLLGAALVWPSSVLLGA
                     AFLAGAFATGLPMLAADVQNARARRAWAREFSRHVHAGGVGELARAHPGGYRLHFREY
                     HGFEMNRLPAAALLENLETGELLPVWPPPKIIETFHAVDGLVVVRH"
     gene            857..2926
                     /gene="recG"
                     /locus_tag="PLP58_21455"
     CDS             857..2926
                     /gene="recG"
                     /locus_tag="PLP58_21455"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:NF008168.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HRJ11162.1"
                     /translation="MPIRPDSPLSEVPGLPARVLRALEKEGVENAARIIEWLPFRHED
                     RRQTGGRVFQPGTTPVCHQVRVTRTGNKFFGSRRGGVFEAQVEQLDDNALGQVLTLRW
                     WGMPFMSRAIAEGQELVVYGRVKDFKGRLSMDHPEYEVLGGEDEPGIHTGRVTPVYRL
                     RAGITQKALRTAAWHVFQSLEPDFTADVLPAPGAQGEFAGWSRARAVRAAHFAESLEE
                     LETARRYLALEEFYTYQLRVVRRRRAVVLSGGHAHPAGELAGRFEAGLPFAMTGAQKR
                     SLEEIQRDMAAPAPMNRLLHGDVGSGKTVVAFAAMLGAVEAGRQAALMAPTQILAEQH
                     FLTARKWLEPLGLGVALRTAARSSEGESFFSDRRADIVIGTHALLYDDELLKNTGLVV
                     IDEQHKFGVAQRARLIQKGGTPDVLVMTATPIPRTLTLTIYGDLDVSTLDERPKERGK
                     IITRVRPASKQDEAARFLIGQLEQGRQAYLVYPIIEESEKLDVTAVTKGCAEWAKRLP
                     HFQTALLHGRMSAEEKEEVMRGFRAGRVDVLVATTVIEVGVDVPNASVMFIHHAERYG
                     LAQLHQLRGRVGRGEHTSYCVLFVKDKDDEARERLAILEETTDGFRISEEDLKRRGPG
                     DVLGRAQSGQAPLQFAELLADTRLVRLARRLAERTLDEDPDLLTPRLAGVRGFVLNEE
                     TPRSAMQ"
     gene            complement(2964..>3883)
                     /locus_tag="PLP58_21460"
     CDS             complement(2964..>3883)
                     /locus_tag="PLP58_21460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009960671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF1588 domain-containing protein"
                     /protein_id="HRJ11163.1"
                     /translation="LLTPDGRAAQVRRMMRDPKAAALAGEFAGQWLEFKGFDKHTAVD
                     EKKFPEFTPELRRDMDRETALFFTHLIREDRPVHEILSADYTFLNERLARHYGVPGVT
                     GEELVRVVVDGRQRGGLLGQGSLLTKTSRSHRTSPVLRGNWLLQAVLGTPVPPPPSDV
                     PELKEHGPKPATVREMLEQHRASKSCSGCHDRIDPLGFALENFDPIGRWREQDEAGLP
                     LDTSARVKGGAPFTGFEGLRGYLRTQQPQFARHFSRKLLGYALGRQVLPTDKALLQEM
                     QAAAGGDDGRFSAAILRVVESRQFLNKRL"
BASE COUNT          570 a         1393 c         1309 g          611 t
ORIGIN      
        1 cctgcgcggc accggcggac gcgtcagcac cctcggttcc gccaccgctc ccgatgtgct
       61 gcaaagccac ctcgccgccg accgcgccgt gctcgtctcc atgccggtgc ctgacgccgg
      121 cgggcgctcg tgggcgcggc tgctcggtgt caatgacagc accgtccgtg tcaccgaccc
      181 cgccacgggt gatcccgtca gctacccgcg ctccgtattt gaccaggtca cgcgcggaca
      241 ggatggctac ttcattctcg acccctggcg cgacaccgag ggtgcgccgc gcaccgaacc
      301 cgcgcccgtt cctcgcgtgc tcgcggacct tgatgaattg ctgacacccg ccgtcgaatg
      361 acgcatgaac ctctcccccg gcagcatcca ctttcctccg cagcgccatt atcgtgtcac
      421 gcgccgtctt ccgctgctgg ccgtctgcct gctgggtgcc gcgcttgtct ggccgtcctc
      481 ggtgctgctt ggagccgcat ttctcgcggg tgctttcgcg acaggtttgc cgatgctggc
      541 ggcggatgtg caaaacgccc gcgcccggcg cgcatgggcg cgtgagtttt cgcgccatgt
      601 ccatgccgga ggcgttggcg aactggcgcg cgcgcatccc ggcggctacc gcctgcattt
      661 ccgcgagtat cacggctttg aaatgaaccg cctcccggcg gcggccctgt tggagaatct
      721 ggagaccggc gagctgctgc ccgtgtggcc tcctcccaaa atcatcgaga cctttcacgc
      781 cgtggatggc cttgtggtcg tcaggcactg atgccgcttg gaaagtgccg cgcctccttc
      841 catgttgggg cgctttatgc ccatccgccc tgactccccg ctctccgaag tgcccggcct
      901 gcccgcgcgg gtgctgcggg cgctggagaa ggagggcgtc gaaaacgcgg cgcgcatcat
      961 cgaatggctg cccttccgcc atgaggaccg ccgccagacg ggcgggcggg ttttccagcc
     1021 gggcacgaca cccgtgtgcc atcaggtgcg cgtcaccagg acgggaaaca aattcttcgg
     1081 cagccgcagg ggcggagtct tcgaggcgca ggtggagcag ctcgatgaca acgcgctggg
     1141 ccaggtgctc acgctgcgct ggtggggcat gcccttcatg agccgcgcca tcgccgaggg
     1201 gcaggagctg gtggtgtatg ggcgcgtgaa ggacttcaaa ggccgcctgt ccatggacca
     1261 cccggagtac gaggtgctgg gcggcgagga cgagccgggc atccacaccg ggcgcgtcac
     1321 gcccgtctat cgcctgcgcg ccggcatcac ccagaaggcg ctgcgcacgg cggcctggca
     1381 tgtgtttcag agcctggagc cggacttcac ggcggatgtg ctgcccgcgc cgggagcgca
     1441 gggcgagttc gccgggtgga gccgcgcgcg cgcggtgcgc gcggcgcact tcgcggagag
     1501 cctggaggag ctggagacgg cgcggcgtta cctggcgctg gaggagttct acacctacca
     1561 gttgcgcgtt gtgcggcgtc ggcgcgcggt ggtgctcagc ggcggccatg cgcatccggc
     1621 gggggagctg gcggggaggt tcgaggcggg gctgcccttt gccatgaccg gagcgcagaa
     1681 gcgctccctg gaggagatcc agcgcgacat ggccgccccc gcgcccatga accgcctgct
     1741 gcatggggat gtgggcagcg gcaagaccgt ggtggccttt gccgccatgc tcggcgcggt
     1801 ggaggccgga cgccaggccg cgctcatggc ccccacgcag atcctggccg agcagcactt
     1861 cctcaccgcg cggaaatggc tggagccgct tggcctcggc gtcgccctgc gcactgccgc
     1921 gcgctccagc gagggggaga gctttttctc cgaccgcagg gcggacatag tcatcggcac
     1981 gcacgcgctg ctttatgatg acgaactgct gaaaaacacc ggcctcgtgg tcattgacga
     2041 gcagcacaag ttcggcgtgg cccagcgcgc gcggctcatc cagaaaggcg gcacgcccga
     2101 tgtcctcgtg atgaccgcca cccccatccc gcgcacgctg acgctgacca tctacggcga
     2161 cctggatgtc tccacgctcg acgagcgccc caaagagcgc ggcaaaatca tcacccgcgt
     2221 gcgccccgcc tcgaagcagg acgaggccgc caggttcctc atcgggcagc ttgagcaggg
     2281 ccgccaggcc tacctcgtct atcccatcat cgaggagtcc gagaagctgg atgtcaccgc
     2341 cgtcaccaag ggctgcgccg agtgggcgaa aaggctgccg cattttcaga ccgccctgct
     2401 gcacgggcgc atgagcgcgg aggagaagga ggaggtcatg cgcggcttcc gcgcgggcag
     2461 ggtggacgtg ctcgttgcca ccaccgtcat cgaggtgggc gtggatgtgc ccaatgccag
     2521 cgtcatgttc atccaccacg ccgagcgtta cggcctggcg cagctccacc agctccgcgg
     2581 gcgcgtcggg cgcggggagc acacctccta ctgcgtgctc tttgtgaagg acaaggacga
     2641 cgaggcccgc gagcgcctcg ccatcctgga ggagaccacc gacggcttcc gcatctccga
     2701 ggaggacctc aagcggcgcg gccccggcga tgtcctcggc cgcgcccaga gcggccaggc
     2761 gccgctgcaa ttcgccgagc tgctcgccga cacccgcctc gtgcgcctgg cacgccgcct
     2821 cgcggagagg actctcgacg aggatcccga tctcctcacc ccgcgccttg ccggcgtgcg
     2881 cggcttcgtc ctcaacgagg aaaccccgcg ctccgccatg cagtgaccgg cattcgcgca
     2941 tgtcaaagca cacgcgccgc ggctcacagc cgcttattga ggaactgccg gctctccacc
     3001 acgcgcagga tggcggcgga aaagcgcccg tcatccccgc ccgccgccgc ctgcatctcc
     3061 tggagcagcg ccttgtccgt cggcagcacc tggcggccca gcgcgtagcc cagcagcttc
     3121 cggctgaagt ggcgggcaaa ctgcggctgc tgcgtccgca ggtagccgcg caggccctcg
     3181 aagcccgtga agggagcgcc gcccttcacc cgggcggagg tgtccagcgg caggcccgcc
     3241 tcgtcctgct cccgccagcg gccgatgggg tcgaagttct ccagggcgaa gcccagcgga
     3301 tcaatgcggt cgtggcagcc cgagcagctc tttgacgccc ggtgctgctc cagcatctcc
     3361 cgcacggtgg ccggcttcgg cccgtgctcc ttcagctccg gcacatcact gggcggcggc
     3421 ggcacgggcg tgcccagcac cgcctgcagc agccagttcc cgcgcagcac ggggctggtg
     3481 cggtggctgc gggaggtctt cgtcagcagg ctgccctggc ccagcaggcc gccgcgctgc
     3541 cgcccgtcca ccaccaccct caccagctcc tctcccgtga cgccgggcac gccgtagtgg
     3601 cgggccaggc gctcattcag gaaggtgtag tccgcgctca ggatctcgtg cacgggccgg
     3661 tcctcgcgga tgaggtgggt gaaaaagagc gccgtctccc ggtccatgtc acgccgcagc
     3721 tcgggcgtga actcggggaa cttcttctca tccaccgccg tgtgtttgtc gaagcccttg
     3781 aactccagcc actgcccggc gaactccccc gccagcgccg ccgccttcgg gtcgcgcatc
     3841 atgcgccgca cctgcgccgc ccggccatcg ggtgtgagca gcg
//
LOCUS       DAOMYJ010000348         3883 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Prosthecobacter sp. isolate AMR_MDS_5629
            NODE_8337_length_3883_cov_16.847962, whole genome shotgun sequence.
ACCESSION   DAOMYJ010000348 DAOMYJ010000000
VERSION     DAOMYJ010000348.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867850
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prosthecobacter sp. (wastewater metagenome)
  ORGANISM  Prosthecobacter sp.
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Verrucomicrobiaceae; Prosthecobacter.
REFERENCE   1  (bases 1 to 3883)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3883)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.30
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:34:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,742
            CDSs (total)                      :: 4,684
            Genes (coding)                    :: 4,676
            CDSs (with protein)               :: 4,676
            Genes (RNA)                       :: 58
            tRNAs                             :: 55
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3883
                     /organism="Prosthecobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8337_length_3883_cov_16.847962"
                     /isolate="AMR_MDS_5629"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1965333"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..285
                     /locus_tag="PLP58_21465"
     CDS             <1..285
                     /locus_tag="PLP58_21465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ11164.1"
                     /translation="GGRRLAGEVAISGAKNAAISLHGGARGISLFAAPDDALLLLAHP
                     VDTSPATLQDWARELLASAGPATAATQPDAPVARGTGVVSLSDALNVSAP"
     gene            285..1379
                     /locus_tag="PLP58_21470"
     CDS             285..1379
                     /locus_tag="PLP58_21470"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HRJ11165.1"
                     /translation="MNLALIRRHFAATGGAELYLQRLIAALAEAGHETHLYAEAWKGA
                     PQGVTLHQVPVRATRASRPPRFAEAVAGQLRGARHDIVLSLERTVSQDVYRAGDGVHA
                     VWLEQRKRYAPWWRRWLIGRGAFHRNMLALEKRVFDPAVTRHVIANSEMVRQEILERF
                     PFPAERVHLVRNGIHTARFQTGERAAARRSLGLEPGHYALLFAGSGWERKGLRFLIAL
                     MRRLEKSAPHVRLVVAGKGRLLRPPPRNVILAGPMPRVEDAYAAADLFTFLPVYEPGS
                     NVVLEALASGLPVITSRFNGAAEWVVEGENGHVLADPADTAGLEAAVRHWMARPGARP
                     VSAPFPLELEANVRGTLEVLERAFEEKRAG"
     gene            complement(1412..1852)
                     /locus_tag="PLP58_21475"
     CDS             complement(1412..1852)
                     /locus_tag="PLP58_21475"
                     /inference="COORDINATES: protein motif:HMM:NF013228.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HRJ11166.1"
                     /translation="MSYQKNAEAQGIVFEKQTPGYLNLCIKSGNQLITSGHVSDLKGK
                     LGAGLTVDEGYRAARNCAEKILKSVWNTHGTLDGLRVIKVLGCVNSTLDFTDQHLVIN
                     GASDLLHVIFGKDGDGYHARSALGFAQLPTGAAVEVEAVFEIAG"
     gene            complement(1976..3205)
                     /locus_tag="PLP58_21480"
     CDS             complement(1976..3205)
                     /locus_tag="PLP58_21480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009959729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1015 family protein"
                     /protein_id="HRJ11167.1"
                     /translation="MRLRSFQGLVPAPQHAAAVAAVPYDVVNREEAYSLAHDNPLSLL
                     HVDRAEIDLPPETDPYSAEVYAKALENFERLQADGVLVREERPCLYLYRQTIGSHSQT
                     GLVGVCHTGDYEGNLIKKHEKTRQDKEDDRTRLVDTLSANTGPIFLTYRGHPAIDRSI
                     AAHMAGHAPLHDFTAPDGVRHEVWPLPVCASAELEEAFASHVPAAYVADGHHRAASAW
                     RVSRQRRSSNHEHNGTEDYNWFLCVLFPGNELNILPYNRVVRDLNGRSPEAFLDEVAG
                     IFKVSPAEEKAPAKPGQCCMYLGGKWHLLEWQPEPDANPVGVLDVSILQDRLLRPVLG
                     IDDPRTSKRVDFIGGIRGTAELERLVDSGAHAVAFSMYPVTVDQLMDIADAGEIMPPK
                     STWFEPKLRSGLFIHTF"
     gene            3352..>3883
                     /locus_tag="PLP58_21485"
     CDS             3352..>3883
                     /locus_tag="PLP58_21485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ11168.1"
                     /translation="MPPQLLFVQKAAGRGGAKNRLLDTLAALQAGADARLHVITGEQG
                     EFTGRLAEMRVPFSVHDLPEWRKPLHRLRFPRAMRRILAALPFDRAAWVVTNEMWWAP
                     HAGALARSLHARSAVIIRDGIATAPKARKYHLHRNDLLLPSSIKIARGLSLDPELAAR
                     TRVFLDSVRVPPATPGG"
BASE COUNT          551 a         1411 c         1276 g          645 t
ORIGIN      
        1 ggaggccggc ggctcgccgg cgaagtcgcg atttccggcg ccaagaacgc ggccatctcc
       61 ctccacggcg gcgcgcgcgg catcagcctc ttcgccgcgc cggatgacgc cctgctgctg
      121 ctggcccatc cggtggacac ctcccccgcc actcttcagg attgggcgcg cgagctgctc
      181 gcctccgcag gtccggccac tgccgccacc cagcctgatg ctcccgtggc ccgcggcacc
      241 ggcgtcgtca gcctttccga tgcgctcaat gtctcagcgc cctgatgaat ctggccctca
      301 tccgcaggca cttcgccgcc accgggggcg cggagcttta cctccagcgc ctcatcgccg
      361 cgctcgccga ggccgggcat gagacgcacc tctatgccga ggcttggaaa ggcgcgccgc
      421 agggcgtgac gctgcatcag gttcccgtgc gcgccacccg cgccagccgc cctcctcgct
      481 tcgcggaggc ggtggccggg cagttgcgcg gcgcgcgtca tgacattgtg ctgagcctgg
      541 agcgcaccgt ctcgcaggat gtctatcgcg ccggggacgg cgtccacgcc gtctggctgg
      601 agcaaagaaa acgctacgcg ccctggtggc ggcgctggct catcgggcgc ggcgccttcc
      661 accgcaacat gctggcgctg gagaagcgcg tcttcgatcc cgccgtcacg cgccatgtca
      721 tcgcgaactc cgaaatggtg cgccaggaga tcctcgagcg cttccctttt ccggcggagc
      781 gcgtccacct cgtgcgcaac ggcatccaca ccgcgcgctt ccagaccggg gagcgcgcgg
      841 ccgcgcgccg cagcctggga ctcgagcccg gtcactacgc gctgctcttt gccggctcgg
      901 gctgggagcg caaagggctg cgctttctga tcgccctcat gcgccggctg gaaaagtccg
      961 cgccgcatgt ccgcctcgtc gtcgccggca aaggccgcct cctgcgcccg ccgccgcgca
     1021 acgtcatcct ggccgggccg atgccgcgcg tggaggatgc ctatgcggcg gcggacctct
     1081 tcaccttcct gcccgtctat gagccgggtt cgaacgtcgt cctcgaggcg ctcgcctccg
     1141 gcctgcccgt catcaccagc cgcttcaatg gcgccgccga gtgggtggtc gagggtgaaa
     1201 acggccatgt cctcgccgat cccgccgaca ccgccgggct ggaggcagcc gtgcgccact
     1261 ggatggcccg ccccggcgcg cggcctgtca gcgcgccctt ccccctggag ctggaagcca
     1321 atgtgcgcgg cacgctcgag gtgctggagc gcgccttcga ggagaagcgc gcgggttgag
     1381 gcgcgcgccc gatccgcgcg gtgcccaccc ctcaccccgc gatctcgaag accgcctcca
     1441 cctccaccgc cgcgccggtc gggagctggg caaagccgag cgcgctgcgg gcgtggtagc
     1501 cgtcaccgtc cttgccgaag atgacgtgca gcaaatccga cgcgccattg atcacgagat
     1561 gctggtccgt gaagtcgagc gtgctgttca cgcagcccag caccttgatc acgcgcagcc
     1621 cgtccagcgt gccgtgggtg ttccagaccg acttgaggat tttctccgcg cagttgcgcg
     1681 ccgcgcggta gccctcgtcc accgtcaagc cagcgcccag tttgcccttc aggtcgctca
     1741 catggccgga ggtgatgagc tggttcccgg atttgatgca gaggttcagg tagccgggag
     1801 tctgcttttc aaagacaatt ccctgggctt cggcgttttt ctggtaggac atggcggggt
     1861 gttgggtggg tgaggattga ggggttaaaa gacgccggca gggcggcctt gcttgcctga
     1921 atggcgggtt ttgtgagacg gaaaggccgc cgcatgatcc ggcgcgcccc cgccctcaaa
     1981 acgtgtggat gaaaagtccc gagcgcagct tcggctcgaa ccaggtgctc ttcggcggca
     2041 tgatctcgcc cgcgtcggcg atgtccatga gctggtccac ggtgacggga tacatgctga
     2101 aagccacggc atgcgctcca ctgtccacca gccgctccag ctccgccgtg ccgcggatgc
     2161 cgccgatgaa gtccacgcgc ttgctggtgc gcgggtcgtc aatgccgagc acgggccgca
     2221 gcaggcggtc ctgcaggatg ctgacatcca gcaccccgac cgggttggcg tccggctccg
     2281 gctgccactc cagcagatgc catttgccgc ccaggtacat gcagcactgg ccgggcttgg
     2341 caggggcttt ttcctccgcc gggctgactt tgaaaatgcc cgccacctcg tcgaggaagg
     2401 cctccgggct gcggccgttg agatccctca ccacgcggtt gtagggcagg atgttcagct
     2461 cgttgccggg gaaaagcacg cagaggaacc agttgtaatc ctccgtgccg ttgtgctcgt
     2521 ggttcgagga gcggcgctgg cggctcacgc gccaggcgct ggcggcgcgg tggtgcccgt
     2581 cggccacata agcggcgggc acatgggagg cgaatgcctc ctccagttcg gcgctcgcgc
     2641 acaccggcag aggccacacc tcatggcgca cgccgtcggg cgcggtgaag tcatgcagcg
     2701 gcgcgtgccc tgccatgtgg gcggcgatgg agcggtctat cgccggatgg ccgcggtagg
     2761 tgaggaaaat ggggccggtg ttcgcgctca gggtgtccac caggcgcgtg cggtcgtcct
     2821 ccttgtcctg gcgcgtcttc tcatgcttct tgatgaggtt gccctcataa tccccggtgt
     2881 ggcagacgcc gacgaggccc gtctggctgt ggctgccgat ggtctggcgg taaaggtaaa
     2941 ggcagggcct ctcctcgcgc acgagcacgc catccgcctg cagcctctcg aagttctcca
     3001 gcgccttcgc atacacctcg gcggagtagg ggtccgtctc cggcggcagg tctatctccg
     3061 cgcggtccac atgcagcagg ctcaagggat tgtcatgggc cagggagtat gcctcctcgc
     3121 ggttgacgac atcatacggc accgcagcca cggcggcggc gtgttgcggg gctgggacga
     3181 gaccttggaa ggagcggagg cgcatggggc gcgcatcctt cacgggcgcg ccgggaagtc
     3241 aacgcccccg gcctgatttt ctcgttggaa gcgggctggt tgaagggtga tggatgctgg
     3301 ccgataggcg gtggcccacc gctgccagcc cgccgcccac cgtcctcccc catgcctccc
     3361 cagctactct ttgtccaaaa agccgccggc cgcggcggcg ccaagaaccg cctgctcgac
     3421 accctcgccg ccctccaggc cggggccgac gcgcgcctcc acgtcatcac cggcgagcag
     3481 ggcgagttca ccgggcgcct cgccgaaatg cgcgtgccct tttccgtcca cgacctgccg
     3541 gaatggcgca agcccctgca ccgcctgcgc ttcccccgcg ccatgcgccg catcctcgcc
     3601 gccctgccct ttgaccgcgc cgcctgggtc gtcaccaacg aaatgtggtg ggcgccccac
     3661 gccggcgccc tggcccgctc cctgcacgcg cgctccgccg tcatcatccg cgacggcatc
     3721 gccaccgccc cgaaggcgcg caagtaccac ctccaccgca acgacctcct gctgccctcc
     3781 tccatcaaaa tcgcccgcgg cctgagcctg gaccccgaac tcgccgcccg cacccgcgtc
     3841 ttcctcgact ccgtgcgcgt gccccccgcc acgcccggcg ggc
//
LOCUS       DAOMYJ010000349         3870 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Prosthecobacter sp. isolate AMR_MDS_5629
            NODE_8383_length_3870_cov_21.762516, whole genome shotgun sequence.
ACCESSION   DAOMYJ010000349 DAOMYJ010000000
VERSION     DAOMYJ010000349.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867850
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prosthecobacter sp. (wastewater metagenome)
  ORGANISM  Prosthecobacter sp.
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Verrucomicrobiaceae; Prosthecobacter.
REFERENCE   1  (bases 1 to 3870)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3870)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.30
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:34:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,742
            CDSs (total)                      :: 4,684
            Genes (coding)                    :: 4,676
            CDSs (with protein)               :: 4,676
            Genes (RNA)                       :: 58
            tRNAs                             :: 55
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3870
                     /organism="Prosthecobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8383_length_3870_cov_21.762516"
                     /isolate="AMR_MDS_5629"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1965333"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..220
                     /locus_tag="PLP58_21490"
     CDS             <1..220
                     /locus_tag="PLP58_21490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009962288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HRJ11169.1"
                     /translation="AAALNAGRIAGAGLDVLSIEPPPPGNPLLTARNCLITPHIGWAS
                     REARIRLIEQTAANLRAYLDGCPVNVVG"
     gene            complement(227..1930)
                     /locus_tag="PLP58_21495"
     CDS             complement(227..1930)
                     /locus_tag="PLP58_21495"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HRJ11170.1"
                     /translation="MPLLVILMLGVTAGIPAQNADLSLPPVDASAARRLQAGGERVAS
                     ALAHYSTALRLEVENNPRAALEHYLRALEVDPSNASLAMHTAELAYSFRGRETAVALL
                     EKTVEANPDAPEPYLNLARFCATYAPDDPFENDRAKLTLDRALKRFPRSAEVHGFAAV
                     TLLSQGQRDEAARVLDGAARQNVSDSQHWLTLGRAAQKVWPLGQAEMREEHTARVNVF
                     FQNALRHAPADDEAARLEVARYYLLTNQLRPARDLCLEISRQTGSLQARKLLHRLHES
                     FEEKEQALRVLEKIVEDAPDDVEYRRMLARAYELREEYARAAPHYETALQKGGGSAED
                     YLHLGETLLRAYLNEKLIEFCKRSAMLFPDQAMFHVQAAMANRALQQWDDAVACFEKA
                     AALAESNQAELVNHRFYFQYGVTLERAGRHEDAGRMFEKSITVTPKEDVEAAASAMNY
                     LGYMWLELDRHFDKAGELILKANELQPETPAYVDSLGWWHFKKGNHTEALRELERAVS
                     LIPDLQAEDAEIIEHIGRVHLAMKQPEKAREAFQKALSLQPKDEKVLRQIKEGLDMAG
                     K"
     gene            complement(2213..3097)
                     /gene="hisG"
                     /locus_tag="PLP58_21500"
     CDS             complement(2213..3097)
                     /gene="hisG"
                     /locus_tag="PLP58_21500"
                     /EC_number="2.4.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012420148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003879 - ATP phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase"
                     /protein_id="HRJ11171.1"
                     /translation="MSEPKNILRLGLPKGSLQEPTLELFKRAGFNVVVSSRSYRPTVD
                     DDELELRLLRAQEIGRYVDHGFLDCGITGRDWIAENNARVEVLTDLRYSRATSKPTRW
                     VLVVPEDSPIKTVKDLQGKRIATEAVDMTKAYLAKHGVEAEVEFSWGATEVKVPELVD
                     AIVDITETGSSLRANKLRIVDTLMESYPQFVSSPPAYRDEWKRRKMETLVLLLKGALE
                     ARDKVGLKMNVPAASLAEVVQALPSERSPTISQLANQEWVAVETVISESVVREIIPRL
                     KSLGAEGIVEYPLNKVVH"
     gene            3297..3701
                     /locus_tag="PLP58_21505"
     CDS             3297..3701
                     /locus_tag="PLP58_21505"
                     /inference="COORDINATES: protein motif:HMM:NF015898.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HRJ11172.1"
                     /translation="MPDKTDITATPRISDAEWTVMKVLWERGTSTLGEVVRELEGRLH
                     WKPRTVQSLIRRLTEKGALAVEAVGREFRYTPAVAQDQCQHEESVSFLDRVFNGRLTP
                     FVAGLMEREEVTKDDLAALREVLAQAEHKLKK"
BASE COUNT          704 a         1291 c         1099 g          776 t
ORIGIN      
        1 ggccgcggcg ctcaacgccg gtcgcatcgc cggcgcgggg ctggacgtgc tctccatcga
       61 gcctccgcca cccggcaacc cgctcctcac cgcgcgcaac tgcctcatca ccccgcacat
      121 cggctgggcc agccgggagg cgcgcatccg gctcattgag cagacagccg ccaatctgcg
      181 cgcttatctc gatggctgcc cggtgaatgt ggtgggatga aacccgtcat ttccccgcca
      241 tgtccaatcc ttctttgatc tgccgcagca ccttttcatc cttcggctgc aaagacaggg
      301 ctttttgaaa agcctcgcgc gccttttcgg gctgcttcat ggcgagatgc acgcgcccga
      361 tgtgctcgat gatctcggcg tcctcggcct gcaaatcggg gatcagcgaa acggcgcgct
      421 ccaattcgcg cagagcctcg gtatgattcc cctttttaaa atgccaccag ccaaggctgt
      481 ccacataggc cggggtctca ggctgcagtt cgttggcctt gaggatcaac tccccagcct
      541 tgtcgaaatg acggtccagt tccagccaca tgtagcccag atagttcatg gcgctcgccg
      601 ccgcctcgac atcctccttg ggcgtgaccg tgatggactt ctcgaacatg cgtcccgcgt
      661 cctcatgcct gccggcccgc tccagggtga cgccgtattg aaaataaaag cggtgattga
      721 cgagctcggc ctggttgctc tccgcgagag cggccgcttt ttcaaagcag gccaccgcgt
      781 catcccattg ctgcagagca cggttcgcca tcgcggcctg gacatgaaac atcgcctggt
      841 cgggaaacag catggcgctg cgtttgcaga actcgatcag cttctcgttc agataagcgc
      901 ggagcagggt ttcccccaga tgcagataat cctcggcgga gccgccgccc ttctggagag
      961 cggtctcgta atgcggggcg gccctggcgt actcctcgcg cagctcatag gcccgcgcca
     1021 gcatgcgcct gtattccaca tcgtccggcg cgtcctcgac gatcttttcc agcacgcgca
     1081 gcgcctgctc tttttcctcg aaggactcgt gcagccggtg cagcagcttg cgcgcctgca
     1141 ggctgcctgt ctgccgggag atttccagac acaaatcccg ggccggacga agctgattgg
     1201 tgagcaaata gtagcgtgcc acctccagcc gggcagcctc gtcatccgcc ggcgcgtgcc
     1261 tgagggcgtt ctgaaaaaac acgttcaccc gtgccgtgtg ctcctcgcgc atctccgcct
     1321 ggcccagcgg ccagactttc tgcgccgccc taccgagcgt cagccagtgc tgcgaatcgc
     1381 tcacattctg ccgcgccgcg ccgtcgagca cacgcgccgc ctcgtctcgc tgcccctggc
     1441 tgagcagggt caccgcggcg aaaccatgca cctccgcgct gcggggaaac cgcttcaatg
     1501 ccctgtccag cgtgagcttg gcgcggtcgt tctcaaaagg atcgtccggc gcgtaagtcg
     1561 cgcaaaaccg cgccaggttc aggtagggct ccggggcatc aggattggcc tccaccgttt
     1621 tctccagcaa agccaccgcc gtttccctgc cccggaaact gtaggccagc tcggcggtgt
     1681 gcatggccag cgaggcgttt gagggatcca cctccaacgc cctgagataa tgctccagcg
     1741 ccgcgcgcgg attgttttcg acctccaggc ggagagcggt cgagtaatgg gcaagcgcgg
     1801 atgccacacg ctcccctccg gcctgcagcc gccttgccgc cgaggcatcc acaggaggca
     1861 gtgagaggtc cgcattctgc gctggaatcc ctgccgtgac gcccagcatg agaatcacca
     1921 gcaagggcat ctggcgcatg aaactccccg ccttcgtgac atgagcaagc gccgtgaaga
     1981 cggcaaaaaa acccggtcgc cggccaatag ccaccccggc gaacgaaaat ggatttctcg
     2041 cagcgtgggg gcggatcatg aggccaaaat ggcgcttacg ctttgtagcg caaacgcttc
     2101 ttgtggcggt tggcgcgcat gcgcttcttg cgcttgtgct tgttgatctt cgtctttctg
     2161 cgctttttca gcgatcccat gttcgtgtgt tctcctgttt tcttgcgccg tctcagtgga
     2221 cgactttgtt cagcgggtat tccacgatgc cttcggcacc gagagatttg agccgcggga
     2281 tgatctcccg cacgacagat tccgagatga ccgtctccac ggccacccat tcctggttcg
     2341 cgagctgcga aatggtcggc gagcgctcgc tgggcagggc ctgcaccacc tcggcgaggc
     2401 tggcggcggg cacgttcatt ttcaggccca ccttgtcacg cgcctccagg gcgcccttga
     2461 gtagcagcac cagggtctcc attttccggc gcttccactc atcccggtag gcgggcgggc
     2521 ttgagacaaa ctgcgggtag ctttccatca gcgtgtccac gatgcgcagc ttgttcgcgc
     2581 gcagggagct gccggtctcg gtgatgtcca caatggcgtc caccagttcc ggcaccttca
     2641 cctccgtggc accccagcta aactccactt cggcctccac gccgtgcttt gccaggtacg
     2701 ccttcgtcat gtccaccgcc tcggtggcga tgcgcttgcc ctgcaaatcc ttgacggtct
     2761 tgatgggcga gtcctccggc accaccagca cccagcgcgt gggctttgag gtggcgcggc
     2821 tgtagcgcaa gtcggtgagc acctcgaccc tggcgttgtt ttccgcgatc cagtccctgc
     2881 ccgtgattcc gcagtccagg aagccgtggt ccacatagcg tccaatttcc tgcgcgcgca
     2941 gcaggcgcag ctccagttca tcgtcatcaa ccgtgggccg gtaggaccgg gaggagacca
     3001 cgacgttgaa ccccgcgcgc ttgaacaatt caagcgttgg ctcctgcagg ctgcccttcg
     3061 gcaggccaag gcggaggatg tttttgggtt cgctcatcag gtcggggaaa agggcgcgca
     3121 aagtagcggg ctttggccgg gtgcaaagga ttatttgggt tcgtaaaaca aaacccaatt
     3181 ttcagcatcc cctgacacca tcccggtctg aagttaagcc cgatgctaaa gtttgagaaa
     3241 aaaatcttgc aatttggatt acatgcgtaa tccttggatc acaaccgtaa tcccaaatgc
     3301 ccgacaaaac tgacatcacc gccacacccc gcatctccga cgctgaatgg acggtcatga
     3361 aggtcctctg ggaacgcggc accagcacgc tcggcgaggt cgtgcgcgag ctcgaaggcc
     3421 gcctgcactg gaaaccgcgc accgtgcagt ctctcattcg ccgcctcacc gaaaaaggcg
     3481 cgctcgccgt cgaagccgtg ggccgtgagt tccgctacac gcccgccgtt gcccaggacc
     3541 agtgccagca tgaagaaagc gtgagttttc tcgaccgagt ctttaatggc cgcctcaccc
     3601 ccttcgtcgc cggcctcatg gagcgcgagg aagtcacgaa agacgacctc gccgcccttc
     3661 gtgaagtgct cgcccaggcc gagcacaaac taaagaagtg agcatacgct caatcgtcct
     3721 cgtcctccta ctcgaactcg tcctcgatcc caacgatctc gtgagaagtc agatgtgaaa
     3781 agtcagaagt agctccgccc cgctgccaat tttcagtttt cagttctcaa ttttcaatcc
     3841 caacccgcgc catgaccgcc gactccctcc
//
LOCUS       DAOMYJ010000350         3870 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Prosthecobacter sp. isolate AMR_MDS_5629
            NODE_8384_length_3870_cov_17.289908, whole genome shotgun sequence.
ACCESSION   DAOMYJ010000350 DAOMYJ010000000
VERSION     DAOMYJ010000350.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867850
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prosthecobacter sp. (wastewater metagenome)
  ORGANISM  Prosthecobacter sp.
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Verrucomicrobiaceae; Prosthecobacter.
REFERENCE   1  (bases 1 to 3870)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3870)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.30
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:34:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,742
            CDSs (total)                      :: 4,684
            Genes (coding)                    :: 4,676
            CDSs (with protein)               :: 4,676
            Genes (RNA)                       :: 58
            tRNAs                             :: 55
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3870
                     /organism="Prosthecobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8384_length_3870_cov_17.289908"
                     /isolate="AMR_MDS_5629"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1965333"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1057
                     /locus_tag="PLP58_21510"
     CDS             <1..1057
                     /locus_tag="PLP58_21510"
                     /inference="COORDINATES: protein motif:HMM:NF012706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HRJ11173.1"
                     /translation="ALRLAFSRGTTAERSRGDAPHAGAGAWRVARRDERSLLLERRDW
                     ESGALPQRLRFFPAQPAHSTRLTLAARGIDIIWPNESGMSAPPPWVEKRPLPSAGQLL
                     LLWNARSPRVADPGLRAALDRMLDRSALAEKNTAQVSDSFFPEGMWFTPPARQRPPEP
                     GAAEQLRNLGWLQDLEGVWKKNGVPLEFTLLAPEDNAPRLALAQAMALQWLDAGARAS
                     IETLPQNSIIGERLATCRFDAVMLGRLNEPGYDMSAWWHSGGRLNYSGLADARLDLLL
                     EALASEFDLTRVPARALAVEERLRELHIALPLLADAPHAAVSRARFAGRPGSTLRELL
                     SPAAPLPPNLKMLEPKE"
     gene            1054..2019
                     /locus_tag="PLP58_21515"
     CDS             1054..2019
                     /locus_tag="PLP58_21515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ11174.1"
                     /translation="MTASLADTSRRLDLTNLMADPPGLLSWNGLGHILSCLWIMMAAL
                     AVSGPAAHGLAAFFINGRAWLRWPARVLAQGLAFLPLTALALAAAGGMAGARGWPVPS
                     LMPLENEPGLASWLWYWTPAVTLLCLPLTAQIFAGLLATEASPRRVLMGAACLAAAGV
                     VMLSETLEILPGMAAPARALRQAHPAFLRHAVAHLALLAGLGTALAALWPAPARKHLT
                     SAADLILEGAVVIGCAPWRLWWRHRLPVLARPAMALVLDALAWCAALAGAWGCLVPLP
                     LLADLHAAGEHFLEQPGALPAAALPVALCSLSLWLAARIVRHGST"
     gene            2026..2865
                     /locus_tag="PLP58_21520"
     CDS             2026..2865
                     /locus_tag="PLP58_21520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009959466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRJ11175.1"
                     /translation="MTPLLRLRDLKIEFRRHDEPPTEAVKGISLELNEGESLAVVGES
                     GSGKSATALALARLLPEPPARITAEELSLAGHDILKMDERALRKVRGREIAYIFQEPT
                     TSLNPVLTVRTQIGEALRLHRPDVTDRDAETLEWLRLVGITEPEKRLRAYPHELSGGM
                     QQRVMIAMALCCRPRLLIADEPTTALDVTIQKQIMDLLAELRRRLGMSLILITHNFGI
                     IRDVADRVAVMYRGRIVETGPVGEILTRPRHPYTQALLECVPRMGARQARLRTIDHAA
                     LEA"
     gene            3005..>3870
                     /locus_tag="PLP58_21525"
     CDS             3005..>3870
                     /locus_tag="PLP58_21525"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HRJ11176.1"
                     /translation="MMPPFTPLAETPRRPPMNIVTEIFRRADPAAIALIENERSVSFG
                     QLQAWCDQAAAQLTGWQGRRAGFYCPNGLAHIVWSLGLLRAGAVLVPVAPELSGPERE
                     QLLATTALHGVLTAGGQNWPHPAGQTRDLKLDDMQARLHSELPPHPAPLFEEKDLAAL
                     NPALIRFSSGTTGKRKGVVIAHQTLLDRVTTANAGLRIGPGDRVVWMLPMAHHFAVSI
                     ILYLLHGATTVLIESHLGADVYAGLKKHGGTVLYAAPFHHALLAAVPDAAPIPSLRLA
                     VSTAAALPEGTAL"
BASE COUNT          570 a         1399 c         1262 g          639 t
ORIGIN      
        1 tgcgttgagg ctggcattca gccgagggac gacagcagaa cgatcgcgag gagacgcgcc
       61 acacgctggc gcgggagcgt ggcgcgtggc gcggagggat gagcgctcct tgctgctgga
      121 gcggcgtgat tgggaaagtg gcgcgctgcc gcaacgtctg cgctttttcc cggcgcagcc
      181 ggcgcattcg acacggctga ccctggccgc gcgcggcatt gacatcatct ggccgaacga
      241 aagcggcatg tccgcgccgc cgccctgggt ggaaaagcgc ccgctgccct ccgccgggca
      301 gttgctcctg ctctggaacg cgcgctcgcc gcgcgtggcg gacccgggcc tgcgcgccgc
      361 gctggatcgg atgctggacc ggtcggcgct ggcggaaaaa aacacagcgc aggtgtcgga
      421 cagctttttc ccggagggca tgtggttcac accgcccgcg cggcagaggc cgccggaacc
      481 cggtgcggcg gaacaacttc gcaacctggg ctggctgcag gacctggagg gagtgtggaa
      541 gaaaaacgga gtcccgctcg agttcacgct gctggccccc gaagacaatg cgccaaggct
      601 ggccctggcg caggcaatgg ccctgcaatg gctcgacgcc ggcgcgcgcg cgagcattga
      661 gacactgccc cagaattcca tcatcgggga aagactcgcc acatgccgct tcgatgccgt
      721 catgctgggc aggctcaatg agccgggtta tgacatgtcc gcctggtggc acagcggggg
      781 gaggctgaac tattccggcc tggctgatgc gcgcctggat ctgctgctgg aggcgctggc
      841 ctcggagttt gacctgaccc gcgtgcccgc gcgggctctg gccgtggagg agcgcttgcg
      901 cgagttgcat atcgcgctgc cgctgctggc agacgcgccc catgccgcag tgagccgggc
      961 gcgttttgcc gggcgtcctg gcagcacgct gcgggagctg ctgagccctg cggcaccgtt
     1021 gcctccaaac ctgaaaatgc tggagccaaa agaatgacag ccagccttgc cgacacctcc
     1081 cgccgcttgg atttgaccaa tttgatggct gatcctcccg gcttgctgtc ctggaacggg
     1141 ctggggcaca tcctctcatg cctctggatc atgatggccg cgctggcggt gtccggcccc
     1201 gccgcgcatg ggctggccgc attcttcatc aacggcaggg catggctgcg ctggcctgcg
     1261 cgtgtgctgg cgcaaggcct ggccttcctc cccctcacgg cgctggcgct tgccgccgca
     1321 ggtggcatgg cgggcgcgcg cggatggcca gtgccgagcc tgatgcctct ggaaaacgaa
     1381 cccggactgg cttcatggct gtggtattgg acacccgccg tcactctgct ctgcctgcct
     1441 ctgacggcgc aaattttcgc cggcctgctc gcaacggaag ccagcccgcg ccgggtgctc
     1501 atgggcgcgg cctgcctcgc cgccgcaggc gtggtgatgc tgtcggagac gttggaaatc
     1561 ctccccggca tggccgcgcc cgcgcgcgct ttgcgccagg cccaccccgc cttcctccgg
     1621 cacgctgtgg cgcatctggc gctgctcgcg ggactgggga cggcgctcgc ggcactgtgg
     1681 cccgcgccgg cgaggaagca cctgacctcc gccgccgatc tcatcctcga gggcgcggtg
     1741 gtcatcggct gcgcgccctg gcgcttgtgg tggcgccacc ggctgccggt gcttgcccgt
     1801 cccgccatgg cgctcgtgct tgacgcgctg gcatggtgcg cggcgctcgc cggagcctgg
     1861 ggctgcctgg tgccgctgcc tttgctggcg gacttgcacg cggcggggga gcactttttg
     1921 gagcagcccg gcgccttgcc cgcggccgcg ctgccggtgg cgctttgctc cttgtcattg
     1981 tggctggcag cgcgcatcgt ccgccacggc tccacttgaa aattcatgac tcccctgctg
     2041 cgcctccgcg atttgaagat cgaattccgc cgtcatgacg agccgccgac ggaggcggtc
     2101 aaaggcatct ctctcgaatt gaacgagggc gagtccctgg ccgtagtcgg cgagagcggc
     2161 agcggcaaaa gcgccaccgc gctggccctg gcgcgcctgc tgccggagcc gcccgcgcgc
     2221 atcacggcgg aggagctttc cctggccggg catgacatat taaagatgga cgagcgcgcg
     2281 ctgcgcaaag tgcgcggcag ggagatcgcc tacatcttcc aggagccgac cacctccctg
     2341 aaccccgtgc tcacagtccg cacgcagatc ggcgaggcgc tgcgcctgca ccgcccggat
     2401 gtcaccgacc gcgacgccga gacgctggag tggctgcgcc tggtgggcat caccgagccg
     2461 gagaagcgcc tgcgcgccta cccgcacgag ctgagcggcg gcatgcagca gcgcgtcatg
     2521 atcgccatgg ccctgtgctg ccgcccgcgc ctgctcatcg ccgacgagcc caccaccgcg
     2581 ctcgatgtca ccatccaaaa gcagatcatg gacctgctcg cggaactgcg ccgccgcctg
     2641 ggcatgagcc tcatcctcat cacccacaat ttcggcatca tccgggatgt ggccgaccgc
     2701 gttgccgtca tgtaccgcgg ccgcatcgtc gagaccggcc cggtggggga aatcctcacc
     2761 cgcccgcggc acccctacac ccaggccctg ctcgaatgcg tgccgcgcat gggcgccagg
     2821 caggcgcgtc tgcgcaccat tgaccacgcc gcgctggagg cttgatgttc cttcacggtc
     2881 atttcagagg cattcatccc gatccagttc cttttgcggg cgtgaaatga caatgcgggc
     2941 aagccctgct tgaacgggcg ggacccttgg gcttggctgg gacgcgggtg ttttcatgaa
     3001 acggatgatg ccgcccttca ccccgctcgc cgagacgccc cgccgccccc caatgaacat
     3061 cgtcaccgaa atcttccgcc gcgccgatcc cgccgccatc gccctcattg aaaacgagcg
     3121 ctccgtcagc ttcggccagc tccaggcatg gtgtgaccag gccgcagccc agctcaccgg
     3181 ctggcagggc cgccgcgccg gcttttactg cccgaacggc ctggctcaca tcgtctggtc
     3241 tctcggcctg ctgcgcgcgg gagccgtgct cgtgcccgtg gcgccggagc tttccgggcc
     3301 tgagcgggag cagctcctcg ccaccaccgc cctgcacggc gtgctcaccg ctggagggca
     3361 aaactggccc catcctgccg gacagacgcg tgatttgaaa ctcgatgaca tgcaggcgcg
     3421 cctgcactcg gagctgccgc cccaccccgc gccactcttt gaggaaaagg acctcgccgc
     3481 gctgaacccc gcgctcatcc gcttcagctc cggcaccacc ggcaaacgca agggcgtcgt
     3541 catcgcgcat cagacactgc tcgaccgcgt caccaccgcc aatgccggcc tccggatcgg
     3601 ccccggcgac cgcgtcgtgt ggatgctgcc catggcgcac cacttcgccg tctccatcat
     3661 cctttacctc ctgcatggcg ccaccaccgt gctcatcgag tcccatctcg gagccgacgt
     3721 ttatgccggc ctgaaaaagc acggcggcac cgtgctctac gccgccccct tccaccacgc
     3781 cctgctcgcc gccgtgccgg acgccgcgcc catccccagc ctgcgcctcg ccgtctccac
     3841 cgccgccgcc ttgccggagg gcacggcgct
//
LOCUS       DAOMYJ010000351         3844 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Prosthecobacter sp. isolate AMR_MDS_5629
            NODE_8473_length_3844_cov_26.517551, whole genome shotgun sequence.
ACCESSION   DAOMYJ010000351 DAOMYJ010000000
VERSION     DAOMYJ010000351.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867850
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prosthecobacter sp. (wastewater metagenome)
  ORGANISM  Prosthecobacter sp.
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Verrucomicrobiaceae; Prosthecobacter.
REFERENCE   1  (bases 1 to 3844)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3844)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.30
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:34:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,742
            CDSs (total)                      :: 4,684
            Genes (coding)                    :: 4,676
            CDSs (with protein)               :: 4,676
            Genes (RNA)                       :: 58
            tRNAs                             :: 55
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3844
                     /organism="Prosthecobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8473_length_3844_cov_26.517551"
                     /isolate="AMR_MDS_5629"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1965333"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..236
                     /locus_tag="PLP58_21530"
     CDS             <1..236
                     /locus_tag="PLP58_21530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ11177.1"
                     /translation="ECAAARLLRAGGRLAGVWFIEPEMDPGEEGPPFALPVAELDALF
                     PAGRWRVVEDEIPTAAYPGRAGRERLRVLERLP"
     gene            233..1375
                     /gene="alr"
                     /locus_tag="PLP58_21535"
     CDS             233..1375
                     /gene="alr"
                     /locus_tag="PLP58_21535"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HRJ11178.1"
                     /translation="MTTFMDAENDSVRPTHVEVDLSALRENLAAIRVRAGAAKVMAVV
                     KANAYGHGLVEVARHMEKCGADCLGVALLEEGLALRKAGVTLPVLVFGGIPAAQMPSM
                     LRHGLEMTAPSVEKLRQMDEAAAAAGIRARVHLKVDTGMGRIGVQYFNARTLIEAGLA
                     CRHVEVAGIYSHFANADAADLSHARLQLERFHEALAVYEKLGERSPMRHIANSAAILQ
                     LPESHLDMVRPGILLYGVQPGPECPLTLPVRPALTWRSRVVYFKVLPSGHPVSYGSTW
                     QSDHPVRVVTVPCGYGDGFPRALSNKAHVLIRGARFPVVGKVCMDQFMVNLGQGTAYN
                     GDEVVLVGAQGGEVIRVEEVAERADTIAYEILTGISSRVPRAHVGD"
     gene            complement(1372..1665)
                     /locus_tag="PLP58_21540"
     CDS             complement(1372..1665)
                     /locus_tag="PLP58_21540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009961927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3817 domain-containing protein"
                     /protein_id="HRJ11179.1"
                     /translation="MSFLRLAALLEAVSYLLLLFIAMPLKYVWAMPLAVRIAGSVHGG
                     LFIVLCLALWRVLLSANWPFSRAALVFAASLLPIVPFLMDGRMKAWADEHASA"
     gene            complement(1749..2735)
                     /locus_tag="PLP58_21545"
     CDS             complement(1749..2735)
                     /locus_tag="PLP58_21545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009959296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase family
                     protein"
                     /protein_id="HRJ11180.1"
                     /translation="MRALTLAGKEDFEWTTLPEPAAPGPGQALVAIRAIGICGTDISG
                     WRGRMPFITCPRILGHELGVEVLATGEDVAHLRAGDRCSVEPYLECGACHSCSRGKTN
                     CCESLEVLGVHCDGGMRERMILPARKLHSCGGLHFEQLALVETLAIGCHAVDRAVLEP
                     EDDVLIIGAGPIGLSVLEFARLAAKRITVFELSAARREFVARHYPGTHVVDSLPDEPV
                     AQVVFDATGSAVSMARAFRHVRFTGRVVYVGITPDPVPLDDPLLHRRELTLLASRNAV
                     SADFPRILRLIQEGRIRTGPWITHRAPLEELPEKMREWTQPEAAVVKAVVAV"
     gene            2817..3218
                     /locus_tag="PLP58_21550"
     CDS             2817..3218
                     /locus_tag="PLP58_21550"
                     /inference="COORDINATES: protein motif:HMM:NF021221.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4870 domain-containing protein"
                     /protein_id="HRJ11181.1"
                     /translation="MESSTPPPDGGQETTSQAGPSKEERTMAMICHLLAFAGFLVPFP
                     GGSIIGPLILWAVKKDGMPFVNEQGKEAINFNITVSIIAVVCMFTFWLILPMILMFAV
                     GVAAIVLIIIAAIQSNEGKHYRYPWILRFVK"
     gene            3234..3569
                     /locus_tag="PLP58_21555"
     CDS             3234..3569
                     /locus_tag="PLP58_21555"
                     /inference="COORDINATES: protein motif:HMM:NF025475.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4282 domain-containing protein"
                     /protein_id="HRJ11182.1"
                     /translation="MPDTEPAPPPAKPAGRPVWGELRAILDLVLDFSFKRFVTPQLIR
                     VLYALSLLGALLGTLAWMFGGFKDGITHGVFTLVTGPVAFVIYVLAARVVMEVILAIF
                     MIAERSRRD"
     gene            3628..>3844
                     /locus_tag="PLP58_21560"
     CDS             3628..>3844
                     /locus_tag="PLP58_21560"
                     /inference="COORDINATES: protein motif:HMM:NF014625.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L family deacetylase"
                     /protein_id="HRJ11183.1"
                     /translation="MRRATFLLAAVTFFAASILPAQDGDGKLRILVFGAHPDDAEYKA
                     GGTAVKWARLGHHVKLVSVTNGDIGHWR"
BASE COUNT          673 a         1231 c         1289 g          651 t
ORIGIN      
        1 acgagtgcgc ggcggcgcgg ctcctgcgcg cgggcggccg cctcgcgggg gtgtggttca
       61 tcgagccgga gatggacccc ggagaggagg ggccgccttt tgccctgccg gtggcggagc
      121 tggacgctct ttttcccgcc gggcgctggc gggtggtgga ggatgaaatc ccaaccgcgg
      181 cctaccccgg acgcgccggg cgcgagaggc tgcgcgtttt ggaaaggctg ccatgacgac
      241 cttcatggac gccgaaaacg acagcgtccg tccgactcat gtcgaggtgg acctttccgc
      301 tttgcgggaa aatctggccg ccatccgtgt ccgcgcgggc gccgcgaaag tcatggccgt
      361 ggtgaaggcc aacgcctacg gccacggtct ggtggaagtg gcgcggcaca tggagaaatg
      421 cggcgcggac tgcctcggcg tggccttgct ggaggagggg ctggctttgc gaaaggcggg
      481 agtcacactg ccggtgctcg tctttggcgg catcccggcc gcgcagatgc cgtccatgct
      541 gcggcacggg ctggagatga ccgcgccctc ggtggaaaaa ctgcgccaga tggacgaggc
      601 ggccgccgct gcgggcatac gcgcgcgcgt gcatctgaag gtggacacgg gcatggggcg
      661 catcggcgtg cagtatttca acgcgcgcac gctcatcgag gccggcctgg cctgccggca
      721 tgtggaggtg gcaggcattt actcccactt cgccaacgcg gacgcggcgg atctctccca
      781 cgcgcggctg caactggagc gcttccacga ggcgctggcc gtgtatgaga agctcggcga
      841 acggtcgccc atgcggcaca tcgccaacag cgcggcgatt ttgcaactgc ctgaaagcca
      901 cctcgacatg gtgcgccccg gcatcctgct ttacggtgtg cagcccggcc cggagtgtcc
      961 gttgaccctg cccgtgcggc ccgctctgac ctggcgcagc cgcgtggtct atttcaaagt
     1021 gctgccgtcc ggccaccccg tcagctacgg ctccacctgg cagagtgatc atcccgtgcg
     1081 cgtcgtcacc gtgccctgcg gttatggcga cggctttccg cgcgctctct cgaataaggc
     1141 gcatgtgctc atccgcggcg cgcgatttcc cgtcgtggga aaggtgtgca tggatcaatt
     1201 catggtgaac ctggggcagg gcacggctta caacggggac gaggtcgtgc tcgtcggcgc
     1261 tcagggcggg gaggtcatcc gcgtcgagga agtggccgag agggcggata ccatcgctta
     1321 cgaaatcctc accggcatca gcagccgcgt gccgagagcg catgtcggcg actaagctga
     1381 cgcatgctca tccgcccacg ccttcatgcg cccgtccatg aggaaaggca cgataggcag
     1441 cagtgaggcg gcgaacacca gcgccgcgcg gctgaaaggc cagttcgcgc tcaagagcac
     1501 cctccacagc gcgaggcaga gcacgataaa aagaccgcca tgcaccgagc cggcgatcct
     1561 caccgccagc ggcatggccc agacatattt gaggggcatg gcgatgaaaa gcagcagcag
     1621 gtaggacacg gcctccagca gggcggccag gcgcagaaac gacacgggat ttttgatcac
     1681 aggccgcgcc tttagcaccg gatgcgggaa gggtgaaagg gaaaacgtgc catcgcggcg
     1741 gatacccctc acacagccac caccgccttc accacggcgg cctcgggctg ggtccactcg
     1801 cgcattttct ccggcagctc ctccagcggc gcgcggtgtg tgatccaggg gccggtgcgg
     1861 atgcggccct cctggatgag gcggaggatg cgcggaaagt cggcggagac ggcgttgcgg
     1921 ctggccagca gggtcagctc gcgccggtgg agcaggggat catccagcgg caccggatcc
     1981 ggggtgatgc cgacatagac gacacgcccg gtgaagcgca cgtggcggaa ggcgcgcgcc
     2041 atggagacgg cgctgcccgt ggcatcaaag accacctgcg ccacaggctc gtccggcagg
     2101 ctgtccacga catgcgtgcc gggataatga cgggccacaa actcccgacg cgccgcgctc
     2161 aactcgaaca cggtgatcct tttggccgcc aggcgcgcaa actccagcac actcaggccg
     2221 atggggcccg cgccgatgat gagcacatca tcctccggct ccagcaccgc gcggtccacg
     2281 gcatggcagc cgatggcgag ggtctcgacg agagcgagct gctcaaaatg gagtccgccg
     2341 cacgagtgta gtttgcgcgc gggcaggatc atgcgctccc tcatgccgcc gtcgcaatgc
     2401 acgccaagga cttcaaggga ctcgcaacag ttcgttttgc cccggctgca ggaatggcag
     2461 gcgccgcatt caaggtaagg ctccacagag cagcggtcgc cagcccgcag atgtgccacg
     2521 tcttcaccag tggcgagcac ctcgacacca agctcgtggc cgaggatccg cgggcaggtg
     2581 atgaagggca tcctgccccg ccagccgctg atgtcggtgc cgcagatgcc aatggcgcga
     2641 atcgccacga gcgcctggcc ggggccggga gcggcgggtt cgggcagtgt ggtccattca
     2701 aagtcttctt tgccggcgag tgtgagcgcg cgcatgggcg catctacgcg cggcggcggc
     2761 gcgcgtcaaa gaccagggtt gcggcgcggc gcgcgcgggc ttagcatccg gcccgtatgg
     2821 aaagcagcac accaccacca gacggcggcc aggagacaac ttcccaggcc ggtccctcca
     2881 aagaggagcg cacaatggcc atgatctgcc acctgctcgc cttcgcgggt tttctcgttc
     2941 cgtttccagg cggctccatc atcgggccgc tgatcctgtg ggccgtcaaa aaagacggca
     3001 tgccttttgt gaacgagcag gggaaggagg cgatcaactt caacatcacc gtgagcatca
     3061 tcgcggtggt gtgcatgttc accttctggc tcatcctgcc catgatcctg atgtttgccg
     3121 tgggcgtcgc cgccatcgtg ctcatcatca tcgccgccat ccagtcgaac gaaggcaagc
     3181 actaccgtta cccctggatt ttgcgttttg tgaaatgacc ctctgatccc gccatgccag
     3241 acaccgagcc agcgccaccc ccggcgaaac ccgccggacg ccccgtctgg ggcgagctgc
     3301 gcgccatcct ggacctggtg ctggactttt ccttcaagcg cttcgtcaca ccgcagctca
     3361 tccgtgtgct ttacgcgctg agcttgctgg gagcgcttct gggcacgctg gcgtggatgt
     3421 tcggcggctt caaggacggc atcacccacg gtgtcttcac cctggtcaca ggaccggtgg
     3481 cttttgtcat ctatgtcctc gccgcgcgcg tggtcatgga ggtgatcctc gccattttca
     3541 tgatcgccga gcggtcacgc agggactgaa taggtgcgag gctgcgcttg cgcgaaggtt
     3601 cgctctgttg tggtgcgtaa gaggaacatg cgccgcgcca ccttcctgct tgccgctgta
     3661 acatttttcg ctgctagcat cctgcccgcc caggacggag atgggaagct gcgtattctc
     3721 gtctttggcg cgcatcccga tgacgcggaa tacaaagccg gtggcactgc ggtgaagtgg
     3781 gcgcgcctcg gtcatcatgt gaaactggtg tccgtgacca atggcgacat cggccactgg
     3841 cgag
//
LOCUS       DAOMYJ010000352         3832 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Prosthecobacter sp. isolate AMR_MDS_5629
            NODE_8509_length_3832_cov_17.424411, whole genome shotgun sequence.
ACCESSION   DAOMYJ010000352 DAOMYJ010000000
VERSION     DAOMYJ010000352.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867850
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prosthecobacter sp. (wastewater metagenome)
  ORGANISM  Prosthecobacter sp.
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Verrucomicrobiaceae; Prosthecobacter.
REFERENCE   1  (bases 1 to 3832)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3832)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.30
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:34:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,742
            CDSs (total)                      :: 4,684
            Genes (coding)                    :: 4,676
            CDSs (with protein)               :: 4,676
            Genes (RNA)                       :: 58
            tRNAs                             :: 55
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3832
                     /organism="Prosthecobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8509_length_3832_cov_17.424411"
                     /isolate="AMR_MDS_5629"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1965333"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(202..885)
                     /locus_tag="PLP58_21565"
     CDS             complement(202..885)
                     /locus_tag="PLP58_21565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ11184.1"
                     /translation="MTRFPRPGCWFRFTQADWNFIRQTLAPDERARRGLDELMQDPES
                     LPLILDNDRLFDAAVVSRERAAPLSPELFFFLVARHALKECGIRELAVADYMAVVCAD
                     YGCAPAKGPEQIERGVDSLYSVDYLEAIENAGRHHRFFLHVQCANQFLVLTCLYPDFL
                     HRRAARRGAPDVDYYEQVVVSHLDSASKHVLAEEFALEETLEKVAECFPPARRAMNHT
                     VREHLSLGQ"
     gene            complement(1113..1463)
                     /locus_tag="PLP58_21570"
     CDS             complement(1113..1463)
                     /locus_tag="PLP58_21570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ11185.1"
                     /translation="MKPLICASCLLTLLASCSAPAPQKPPQVAGQPVPDRNVVLDFTK
                     RYNLEYREGDETRKIQNCRIQGYTGEAVRDATGALSKSYYGDFGRWLVLELADGRLAC
                     LPGSRVSYLEESRR"
     gene            complement(1956..2420)
                     /locus_tag="PLP58_21575"
     CDS             complement(1956..2420)
                     /locus_tag="PLP58_21575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ11186.1"
                     /translation="MNQMLLLALFFVSALSISQGQTNFDYGPIRADKLKRPYTSSFYD
                     DFITARITMKSEIVKINLKVSAYFYGPDNKLLYSSAGHVRGLTEGETPSSYLYPNKKY
                     LAVVQEQPQKAKKWRHVVLVIGMPGDLWARVYPNDDLAKYSFPEKDSVSVAR"
     gene            complement(2475..2939)
                     /locus_tag="PLP58_21580"
     CDS             complement(2475..2939)
                     /locus_tag="PLP58_21580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ11187.1"
                     /translation="MMKSYMLLFILLALAAPAAQGQYQLGPVTLRNVTSTQSGFICNV
                     SVPSDMDPTVLVRKAYIFNTENKRILTIIGGTEDPREMNTRNYTAPDMFKGGKKYPIF
                     FMLPPEFESGRRRWKHAVVVFGKGNDLVARVYPQDELKKYNFPEKGIAKLVE"
     gene            complement(3244..>3832)
                     /locus_tag="PLP58_21585"
     CDS             complement(3244..>3832)
                     /locus_tag="PLP58_21585"
                     /inference="COORDINATES: protein motif:HMM:NF012264.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HRJ11188.1"
                     /translation="LIRLIASARHPAPEALATAAQVLDHPMDKFLDYALKQAVKARQL
                     IPGQAAFARPAHAEYVKKIATAAPVAAHPGKAIYDALCLNCHQPEGKGLPGIYPSIAA
                     SDWVNGDPARLIQITLHGLSGPIQVNGQPFAQAAPLPMPPMGLDDRQTADVLTWLRAS
                     FGNDAPPITPAEVQKVRAAHAARAAFWTAEELGAR"
BASE COUNT          717 a         1140 c         1207 g          768 t
ORIGIN      
        1 ggggaaggcg gcggccccac cgcgcggcag gtcttcgacc atctggccgc cgggcgcatc
       61 cacccctccc tgcgccacga ccgctccggg cgcgagcttc tgcgcttcag cccgcattac
      121 tacaacacac tggaggaaat ggaccgcgtg gccgccgccg tggccgccgc ctgaggactc
      181 gcgcgcgccg gggccggcgc ctcactggcc caggctcagg tgctcgcgca cggtgtggtt
      241 catggcgcgg cgcgcgggcg ggaagcactc cgccaccttc tccagcgtct cctccagcgc
      301 gaactcctcc gccagcacat gcttgctggc cgagtcgagg tggctcacca ccacctgctc
      361 gtaatagtcc acatccggcg cgccccggcg cgcggcgcgg cggtgcagga agtccgggta
      421 gaggcaggtg agcaccagga actggttggc gcactgcaca tgcaggaaga acctgtggtg
      481 ccgcccggcg ttctcgatcg cctccaggta gtccacgctg tacaggctgt ccacgccgcg
      541 ctcgatctgc tccggcccct tcgccggcgc gcagccgtag tccgcgcaca ccaccgccat
      601 gtagtcggcc accgccagct cgcggatgcc gcattccttc agagcgtggc gcgccacgag
      661 aaagaaaaac agctccggcg aaagcggggc cgccctctcc cgcgagacca ccgccgcgtc
      721 aaagagccgg tcattgtcca ggatcagcgg cagcgactcc gggtcctgca tcagctcgtc
      781 cagcccgcgc cgcgcccgct cgtcgggcgc cagggtctgg cggatgaaat tccagtccgc
      841 ctgggtgaag cggaaccagc agccggggcg ggggaagcgc gtcataaatg caagggggtg
      901 accggaaaca catcagcagg gccgatgcca gccggaacgc cgatttggaa agaccccgcc
      961 ggatctctgt tcaaaatcca ccctgcggcg gacggcacca cctgacgcgc tcaaaatcag
     1021 ccgtttggag cctggaaaag ccgcatccca ctctggcccc cctcctttcg gtcggaaatg
     1081 ggggcgggaa accgccgaag ggcgggagag gctcatctcc ggctctcctc cagatagctg
     1141 accctggagc cgggcaggca ggcaagacgc ccgtcagcga gctcgagcac gagccagcga
     1201 ccaaagtccc cgtagtagct tttggagaga gcgccggtgg catcacgcac ggcttctccg
     1261 gtgtagcctt ggattctgca gttttggatt ttgcgcgtct cgtctccctc gcggtattcc
     1321 aggttgtagc gcttggtgaa atccaggacc acattcctgt ccggcacggg ctgcccggcc
     1381 acttgcggcg gcttctgcgg tgctggcgcc gagcacgatg cgagcagtgt gaggagacag
     1441 gatgcgcaaa tcagcggctt catggttggt gaaataaatc agcttctttg tatgcggcgg
     1501 gaaggaattt gtcgagatgc atccatccct cccaggcagc cgggcccggt gccaccccgt
     1561 ttcaggctgc ccgccgcttt gaggcgtgtt tgaaaacccc aagctcctgg tgcggagaag
     1621 caaaggagtc ctcccgatcc gcgggaggaa cgttgcgctt ccagcaggcg gtcatgtccc
     1681 caggcgttgg caaagcgtgg tctgtgcggc cccgaggctc atctcgccga gcgggatggc
     1741 ggccgtgctg gcgcgttttc cggcatgccc gcgcatgttc gggaggtttg caaacacagc
     1801 ccagggcact ttcaccgcgt gccgagctcc tccgctgtcc tttgggagaa taggcgtggg
     1861 tgacccgccg tagcagggca ccgtctatct gtcagtggaa gagttttgct ttgaaccgac
     1921 ttggcttact cccgccattg agcagcgaag taagctcaac gcgccacaga aacagaatcc
     1981 ttctccggaa agctatactt cgccaaatca tcattcggat acacacgagc ccataggtca
     2041 cctggcatgc cgataacaag gacgacatgc ctccacttct tcgctttctg tggttgctcc
     2101 tgcacaacag ccaaatactt cttgttaggg tagaggtatg aactcggggt ctcgccttcg
     2161 gtcagaccac gaacgtgtcc ggcacttgaa taaagcaact tattgtcagg tccatagaaa
     2221 tatgcgctca cctttaaatt gattttcaca atttctgact tcatcgtaat tctggcagta
     2281 atgaaatcat catagaacga cgaggtatac ggtcttttga gtttatcagc tctgatggga
     2341 ccgtagtcaa aattggtctg gccttgagaa atcgaaagcg cgctcacaaa gaaaagcgcg
     2401 agcagaagca tctggttcat ggcgttaggt ggttggaatt ttcgtcttta tatgctgggt
     2461 tggacgatct gcatttactc aactagcttg gcgataccct tttcggggaa attatacttt
     2521 tttagttcgt cttggggata aactcgcgcc accaaatcgt tccccttgcc aaaaacgacc
     2581 acggcatgtt tccatctgcg tcgaccggat tcaaattcag gtgggagcat gaagaaaatt
     2641 gggtatttct tccccccctt gaacatgtca ggtgccgtgt aatttcttgt attcatttct
     2701 cgcgggtctt ccgtccctcc gataatggtc aaaatgcgct tgttttcagt attgaaaata
     2761 tacgccttcc tgacgaggac agtgggatcc atgtcggaag ggacggacac attgcatata
     2821 aacccactct gtgtgctcgt gacattcctg agagtgacgg ggcctagttg atactgccct
     2881 tgggcagcag gtgcggctag ggccagcaaa atgaaaagca gcatgtatga tttcatcatt
     2941 ttattaataa tatcatgctt aagttacttg tcgccgcaat gtcaactctt atatcgtgct
     3001 tgcccgccgc tttgaggcgt gtttgaaaac cccaagctcc gggtgtggcg aagcaaagaa
     3061 gtcctcccga tccgcgggag gaaggttgcg cctccacagg cagtcatgtc cccaggcgtt
     3121 ggcaaagcgt ggtctgtgcg gccccgaggc tcatctcgcc gagcgggatg gcggccgtgc
     3181 tggcgcgttt tccggcatgc ccgcgcatgt tcgggaggtt tgcaaacaca gcccagggca
     3241 ctttcaccgc gcgccgagct cctccgctgt ccagaaggcg gcgcgcgcgg catgggcggc
     3301 gcggactttt tggacctcgg cgggggtgat gggcggggcg tcgttgccaa agctggcgcg
     3361 cagccaggtg aggacatctg cggtctgccg gtcatcgagg cccatgggcg gcatgggcag
     3421 cggggcggcc tgggcgaagg gctggccgtt cacctggatg gggccgctga ggccgtggag
     3481 ggtgatctgg atgagccgcg ccgggtcgcc attcacccag tcgctggcgg cgatgctggg
     3541 gtaaatgccg ggcaggccct tgccttcggg ctggtggcag ttcaggcaaa gggcgtcgta
     3601 gatggccttg ccgggatgcg cggccacggg cgcggcggtg gcgatctttt tcacatactc
     3661 ggcgtgggcg ggacgggcaa aggcggcttg tccggggatg agctggcggg ctttgacggc
     3721 ctgcttcagg gcgtagtcga ggaacttgtc catggggtga tccagcacct gcgcggcggt
     3781 ggcgagcgcc tcgggcgcag ggtggcgcgc ggaggcgatg aggcggatga gc
//
LOCUS       DAOMYJ010000353         3819 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Prosthecobacter sp. isolate AMR_MDS_5629
            NODE_8562_length_3819_cov_20.345377, whole genome shotgun sequence.
ACCESSION   DAOMYJ010000353 DAOMYJ010000000
VERSION     DAOMYJ010000353.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867850
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prosthecobacter sp. (wastewater metagenome)
  ORGANISM  Prosthecobacter sp.
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Verrucomicrobiaceae; Prosthecobacter.
REFERENCE   1  (bases 1 to 3819)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3819)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.30
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:34:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,742
            CDSs (total)                      :: 4,684
            Genes (coding)                    :: 4,676
            CDSs (with protein)               :: 4,676
            Genes (RNA)                       :: 58
            tRNAs                             :: 55
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3819
                     /organism="Prosthecobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8562_length_3819_cov_20.345377"
                     /isolate="AMR_MDS_5629"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1965333"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            223..2958
                     /locus_tag="PLP58_21590"
     CDS             223..2958
                     /locus_tag="PLP58_21590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009963735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HRJ11189.1"
                     /translation="MRLGFIPLVFLAVSSAAAQSPAKPGTAPVTPWGDFVEKDFPFFS
                     SVLDCRDLGEGFPKDNLTPRGLILNVGSERGGGNLWACFDTELLRLAFVWSGREKSPV
                     LPAALATGSYHVAGQKTKDGQEDLPEPGPSVGPVLSNGVYPGWQVLQSASGKPVFHDP
                     REPAPSPEEVGRGPLDEATGRFESVTVVDGAARLSYRVGGTRVTERLEMVNDSGSSLF
                     VRHFDIEPHDKPLALVYAHEANVDDARFLVGEGRDPSSLKVCQNQMEKDSGVFWLKVE
                     ASPVPHSLTIQYGAFPARGFVASNHPAAKEKSQPHWSETVTTHGVLSTKPDAHVVDTI
                     PLPVPNPWKRNVRLADLDFLDDQGNAAGVTFDGDVWLISGLKGDLENVTWKRFASGLH
                     EPMSIVARKNSKLETRTSNLDILVFDRNGIWKLVDSDNDGVCDRYEMFCNLFAQTAET
                     REFPNSMKLGPDGALYISKGGQEGTTRGKHNGTVIKVAADGKSFEVIGHGLRQPFIGV
                     HPRTGLVTASDQQGHYVPSTPLQIIEKNQFYGHLPTIAPKEQYPEPIAEPAAWIPHPV
                     NPSGATQTWLVDAKMGPVNDELIHVGYNRPEIFRVVMNTRFARPQAAVMSFSREFDFG
                     PLNAKVNPADGQLYVVGFQVWGTTAKQLSGLARVRHTGKPRVLLKEVTPMDKGVLLRF
                     NAPLDAKLAADPASYSAERWNYKRSAEYGSPHLKLDGSAGQEFMTASSAYLSKDGQSV
                     LVGIPDMQGGVQQMRIGWGLKSADGLPANNTAYFTPWELAVFDAKKEGFDDIQVDLTP
                     RAAVAMTQAKASAEEGERVAQMFGCMACHSIDGTTVGKVGPSWKGLHGSEREIAKGMK
                     GKVTADADYLRESILAPSAKVVKGFEKFDTGMPIYAGILNESQIESLILYIKSLE"
     gene            complement(2999..>3819)
                     /locus_tag="PLP58_21595"
     CDS             complement(2999..>3819)
                     /locus_tag="PLP58_21595"
                     /inference="COORDINATES: protein motif:HMM:NF024799.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HRJ11190.1"
                     /translation="DREAGLSLAHAGGSAARQMDQMLDALKKKPAFVIVSPVDAAMLT
                     PLVLEARKNGALVIGIGENARQAPCDTVLWCDPQKLGRLAGEVAVRALKRRAEAESAD
                     EARGRVLQIRGDENSARCTGMDAGFQAALKEAPGVVLVHDAPGFWTREGGEARARDAL
                     RIQRVVDVIYAHNDLMALGAVEALGGSRDDVLVIGTDGLAGKNGGYTLVNEGVLDATI
                     YQPLLVDFAALIVAKKMQDPAFEPRKEYELVPRSVMPRDLDDIRLKGMMPYPDL"
BASE COUNT          698 a         1206 c         1227 g          688 t
ORIGIN      
        1 ggcaatcaag ccgcgcgggc ggcgtcggga agctggtctg tcagggtgca tgggttcaac
       61 ggatcaagcc aacgactcaa ggggcgcgaa agtcgcggaa aagtgtaggc cgtccaggcc
      121 aaatcgcaat cattgatcca tcttcccggc atttcaccag agggttgcgg atgaaaatct
      181 ctcccgctga cagacgcggc gttttcggct tgtttcttga cgatgcgcct tggcttcatt
      241 cctctggttt ttctggctgt ttcatccgct gccgcccaat ctcccgccaa acccggcacc
      301 gcgccggtga cgccctgggg cgacttcgtg gagaaggatt tcccgttttt cagcagcgtg
      361 ctcgactgcc gcgacctcgg cgagggcttc cccaaggaca atctcacccc gcgcggcctg
      421 attctgaacg ttggcagcga aaggggtggc gggaacctct gggcctgctt tgacacagaa
      481 ttgcttcgcc tggcttttgt ctggtcaggc cgggaaaaaa gccccgtctt gcctgctgcg
      541 ctcgccaccg gctcctacca tgtcgccggc cagaagacga aggacggcca ggaggatttg
      601 cccgagccag gaccgtccgt cggtcctgtt ttgtccaatg gcgtttatcc cggctggcag
      661 gtgctccagt cagcttccgg caaacctgtc ttccatgatc cgcgcgaacc tgcgccgagc
      721 cctgaagaag tgggtcgcgg gccgcttgat gaagcaacgg gccgcttcga gtcggtcacc
      781 gtcgtggacg gagcggccag actttcttac cgggttggag gaacgcgggt gaccgagcgg
      841 cttgagatgg tgaacgactc cggctctagt ctttttgtcc ggcactttga cattgagccg
      901 catgacaagc ccctggccct ggtctatgcc catgaggcga atgtggatga cgctaggttt
      961 ctggtcggcg agggccgtga tccctcgtca ctcaaggtgt gtcaaaacca gatggagaag
     1021 gattcaggcg tcttctggct gaaggtggag gcctcgcctg tgccacattc gttgacgatt
     1081 caatacggtg cctttcccgc acgcggcttc gtcgccagca atcaccctgc cgccaaagaa
     1141 aaatcgcaac cgcattggtc tgaaaccgtc accacccacg gcgtgctctc caccaagccc
     1201 gacgcccacg tcgtggacac catcccgctg ccggtgccaa acccgtggaa gcgcaacgtg
     1261 cgtctggccg acctcgattt cctcgacgac cagggcaatg cggcgggcgt caccttcgac
     1321 ggcgatgtct ggctcatctc cgggctgaag ggcgatctcg aaaacgtgac ctggaagcgc
     1381 ttcgcctccg ggctgcatga gccgatgagc attgtggcac ggaaaaattc gaagctcgaa
     1441 actcgaactt cgaatttgga catcctcgtc ttcgaccgca acggcatctg gaagctcgtg
     1501 gactccgaca acgacggtgt gtgcgaccgc tatgagatgt tctgcaacct cttcgcgcag
     1561 acagcggaga cgcgggagtt tccgaactcg atgaagctcg gcccggatgg agcgctctac
     1621 atttccaagg gcggccagga gggcacgacg cgcggcaagc acaacggcac cgtcatcaag
     1681 gtggcggcgg atgggaagtc cttcgaggtc atcggccacg gcctgcgcca gcccttcatc
     1741 ggcgtgcatc cgcgcactgg cctcgtcacc gccagcgatc agcaggggca ttacgtgccc
     1801 tccacgccgc tgcagatcat tgagaaaaac cagttctacg gccacctgcc caccatcgcg
     1861 ccgaaggagc agtatccgga acccatcgcc gagccggcgg cgtggattcc gcatcccgtg
     1921 aatcccagcg gcgccacgca gacctggctg gtggacgcga agatgggtcc ggtgaatgac
     1981 gaactgatcc atgtgggcta caacaggccg gaaatcttcc gcgtggtgat gaacacacgc
     2041 tttgccaggc cgcaggccgc cgtgatgagc ttctcgcgcg agttcgactt cggcccgctg
     2101 aacgcgaagg tgaatcccgc cgacggccag ctttatgtcg tggggttcca ggtctggggc
     2161 accacggcca aacagctcag cggtctggcg cgcgtgcgcc acaccggcaa gccgcgcgtg
     2221 ctgctgaagg aggtcacccc catggacaaa ggcgtgctgt tgcgcttcaa cgcgccgttg
     2281 gatgcgaagc tggccgccga tcccgccagc tacagcgccg agcgctggaa ttacaagcgc
     2341 agcgccgagt atggatcgcc gcatctgaag ctggacggca gcgccgggca ggagttcatg
     2401 accgccagca gcgcctacct gagcaaggat ggccaaagcg tgcttgtggg catcccggac
     2461 atgcagggcg gcgtgcagca gatgcgcatc ggctggggcc tcaagagcgc cgacggcctg
     2521 cccgcgaaca acaccgccta cttcacccct tgggagctgg ccgtctttga tgcgaaaaag
     2581 gagggctttg acgacatcca ggtggacctc accccgcgcg cggcggtggc gatgacgcaa
     2641 gcaaaagcca gcgccgagga gggcgagcgc gtggcgcaga tgttcggctg catggcctgc
     2701 cacagcatag acggcaccac ggtgggcaag gtggggccga gctggaaggg cctgcacggc
     2761 tccgagcgcg agatcgccaa aggcatgaag ggcaaagtga ccgccgatgc ggactacctg
     2821 cgcgagtcca tcctggcccc gtccgccaag gtggtgaaag gtttcgagaa gttcgacacc
     2881 ggcatgccga tttatgcggg catcctcaat gagtcgcaga tcgaaagcct catcctttac
     2941 atcaagtcgc tggagtgagc gtgcgggtcg gaggcgaaac ccgtgccact atgcggtgct
     3001 ataaatccgg gtagggcatc atgcccttga ggcggatgtc gtcgaggtcc cggggcatga
     3061 cggagcgcgg gaccagctcg tattccttgc gcggttcgaa ggcggggtcc tgcatttttt
     3121 tggcgacgat gagggcggcg aagtccacga gcagtggctg gtagatggtg gcgtcgagca
     3181 cgccctcatt gacaagggtg tagccgccgt ttttgcccgc caacccatcc gtgccgatga
     3241 cgagcacatc gtcacggctg cctccgaggg cctcgacggc gccgagggcc atgaggtcgt
     3301 tgtgggcata gatgacatcc accacgcgct ggatgcgcag cgcgtcccgc gcgcgcgcct
     3361 cgccgccctc gcgcgtccag aagccgggcg cgtcatgcac gagcacgact ccgggcgcct
     3421 ctttgagggc ggcctggaat cctgcgtcca tgcccgtgca gcgcgcggag ttctcatctc
     3481 cgcggatttg cagcaccctg ccgcgcgcct cgtccgcgct ctccgcctcg gccctgcgct
     3541 tgagcgcgcg cacggccacc tcgcccgcga gcctgccgag tttttgcgga tcgcaccaga
     3601 gcacggtgtc gcagggggcc tggcgcgcgt tttcaccgat gccgatgacg agggcgccgt
     3661 tttttctggc ctccaggacc agcggcgtga gcatggcggc atccaccggc gagacgatga
     3721 caaaggcggg cttttttttc aacgcgtcga gcatctggtc catctgccgc gcggcgctgc
     3781 cgccggcatg ggcaagggag aggcccgcct cgcggtccc
//
LOCUS       DAOMYJ010000354         3784 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Prosthecobacter sp. isolate AMR_MDS_5629
            NODE_8662_length_3784_cov_20.616251, whole genome shotgun sequence.
ACCESSION   DAOMYJ010000354 DAOMYJ010000000
VERSION     DAOMYJ010000354.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867850
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prosthecobacter sp. (wastewater metagenome)
  ORGANISM  Prosthecobacter sp.
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Verrucomicrobiaceae; Prosthecobacter.
REFERENCE   1  (bases 1 to 3784)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3784)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.30
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:34:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,742
            CDSs (total)                      :: 4,684
            Genes (coding)                    :: 4,676
            CDSs (with protein)               :: 4,676
            Genes (RNA)                       :: 58
            tRNAs                             :: 55
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3784
                     /organism="Prosthecobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8662_length_3784_cov_20.616251"
                     /isolate="AMR_MDS_5629"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1965333"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(487..876)
                     /locus_tag="PLP58_21600"
     CDS             complement(487..876)
                     /locus_tag="PLP58_21600"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009960203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HRJ11191.1"
                     /translation="MNHPLVSRPQLPGTPASHLPFSPCVVVGDLIFVSGQASVDAQGN
                     IISDSFEGEFRRSIENVRKVLEAAGSDLSHVVQTRNYVRDDADLPLYNQLYREYFPAP
                     CPARTTLTHCLPPTLKYEIEAVAVRKA"
     gene            complement(1058..2773)
                     /gene="ilvD"
                     /locus_tag="PLP58_21605"
     CDS             complement(1058..2773)
                     /gene="ilvD"
                     /locus_tag="PLP58_21605"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009958912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004160 - dihydroxy-acid dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009099 - valine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HRJ11192.1"
                     /translation="MSKKAASNASLHRKHSAAVVDGVERAASRAMLHAVGFKREDFKK
                     NKIGIASTWSMVTPCNMHIDRLAKEAAKGVDAAGGKSVIFNTITISDGISMGTEGMKY
                     SLVSREVIADSIETVVGCEGMDGYVAIGGCDKNMPGCIMAMGRMNRPSVFVYGGTILP
                     GCVGPEKKDADVVTVFEAIGKHANCQINDKELQDIEEHAIPGEGSCGGMYTANTMASA
                     IEALGMSLPNSGAQSAVSNEKLIDCFDAGAAVMNMIKLGITPRDIMTKEAFENAITLI
                     IALGGSTNAVLHLIAAAHSTGVKLSIEDFVRIGKKTPVLADLRPSGKYFMNDLVKIGG
                     TVPLMRMLVEEGLMHGGCLTVTGRTMKENMQRSKIVWPKSQTIVRPLSNPIKKDSHLV
                     IFKGNLCPDGAVGKITGKEGTKFSGKAIVFESEEKALQAILTDKVKKGHVIVVRMEGP
                     KGGPGMREMLSPTSAIMGKGLGKDVAFITDGRFSGGSHGFVVGHVTPEAFVGGPIAVI
                     KNGDPITIDAEKRQITLGIPDKELKARLKAWKQPKPRYTRGVLAKYAKLTSDASHGAV
                     TDLGL"
     gene            2893..>3784
                     /locus_tag="PLP58_21610"
     CDS             2893..>3784
                     /locus_tag="PLP58_21610"
                     /inference="COORDINATES: protein motif:HMM:NF019040.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1501 domain-containing protein"
                     /protein_id="HRJ11193.1"
                     /translation="MHTRRHFLTRSGFGLGSLALAGVLNDMGLTRANAENLSPLAPRR
                     PHFPGTAKRVIHLFMNGGPSQIDTFDPKPLLKKFHGKSLPSAKNLAKDRRLGGAALAS
                     PFEFKKHGQSGMEISELFPHVARHADDLCVIRSMFSDVPNHEPGLMIMNTGNFTLPRP
                     SVGSWALYGLGTENQSLPGFVVMCPNGLPTAQSANWRNAFLPGIYQGTHIDTKETTPD
                     QLIANLRNDFILPGQQRKQLGFIQKLNEMHRAQRAGDPQLEARIQSLELAFRMQTEAT
                     EAFDVEQEPANIREMYGDTLQ"
BASE COUNT          658 a         1114 c         1258 g          754 t
ORIGIN      
        1 gcagcgctga ctgtccggca cgcgtcgact gtacccgctg gccaatgttg agcatccgct
       61 ccgcgcaggt ggctgcgcaa tcccttctcc agcacgccca gcgccccgca ataccagggt
      121 ggcggcggtc cgccgccggg gttgcagacc ttttgaaggc atggaaaaaa ttggccaagt
      181 ttgggccggc attgcctcac aggggaaatg aggcggaagg gcggcgtgag gaagccgcag
      241 ccctccaacg ttcgcagacg gacctcctgc ggcggtagaa agagccgtca atgacacatt
      301 ttgaaaggct ccaacaaaga acaaaaattt tcaacagatg acccggaatt ccattttcac
      361 cccgcttttt gagatggctg tcaccctttt caaacagcca tccttttgat gaattggccc
      421 ggtattcccc ctgggccatg atatggcagg gttgtgacgg atgtcggtgg ccggaggtca
      481 gggcggtcag gcttttcgga cggcgacggc ttcgatctcg tatttgaggg tgggtggcag
      541 gcagtgggta agtgtggtgc gcgcggggca gggcgcggga aaatattcac ggtaaagctg
      601 gttgtagagg ggcaggtcgg cgtcgtcgcg gacatagttg cgggtttgca cgacgtggct
      661 gagatcgctg ccggcggctt cgagcacttt gcgcacgttc tcgatgctgc ggcggaactc
      721 gccctcaaaa ctgtcgctga tgatgtttcc ctgcgcgtcc acggaggcct ggccggagac
      781 gaagatcaaa tcaccgacaa cgacgcaggg gctgaagggc agatgcgagg cgggtgtgcc
      841 gggaagctgc gggcgggaga cgagggggtg gttcatgcgg gagggcaggg agcgggggag
      901 gcgcgggctt tggattcggg acgggctttt tccccgtccc ggcgctttgg ctggcaggcg
      961 gggaatgcca ccgggaggag cgggaaaaaa ggatactgga atgacagcgc ggatgccgcg
     1021 agcccggcgc gcgcggcatg gcagcgccag cctcaagtca cagccccagg tccgtcaccg
     1081 cgccatggct ggcgtcgctg gtgagcttgg cgtacttggc gagcacgccg cgggtgtagc
     1141 gcggtttggg ctgtttccag gctttgaggc gggctttgag ctccttgtcg gggatgccga
     1201 gcgtgatctg gcgtttctcg gcgtcgatgg tgatggggtc gccgttcttg atgacggcaa
     1261 tcgggccgcc gacgaaggct tcgggcgtga cgtggccgac gacgaagccg tggctgccgc
     1321 cgctgaagcg tccgtcggtg atgaaggcga catctttgcc caaacccttg cccatgatgg
     1381 cggaggtcgg cgagagcatc tcacgcatgc cggggccgcc tttcgggcct tccatgcgca
     1441 cgacgatgac gtggcctttt ttgactttgt cggtcaaaat ggcctgcagg gctttttcct
     1501 cgctctcgaa gacgatggcc ttgccgctga acttcgtgcc ttccttgccc gtgatcttgc
     1561 cgacagcgcc atcggggcag aggttgcctt tgaagatgac gaggtggctg tccttcttga
     1621 tgggattgga cagcggacgg acgatggtct ggctctttgg ccacacgatc ttcgagcgct
     1681 gcatgttttc cttcatcgtg cggccggtga cggtgaggca gccgccgtgc atgaggcctt
     1741 cctcgacgag catgcgcatg agcgggaccg tgccgccgat tttgacgagg tcgttcatga
     1801 aatatttgcc gctggggcgc agatcggcca gcacgggcgt ttttttgccg atgcggacga
     1861 agtcttcgat gctgagcttc acgccggtgc tgtgcgcggc ggcgatgagg tgcaagacgg
     1921 cgttcgtgga gccgccgagc gcgatgatga gcgtgatggc gttctcaaag gcctccttcg
     1981 tcatgatgtc gcgcggcgtg atgccgagct tgatcatgtt catgacggcc gcgccggcgt
     2041 cgaaacagtc gatgagcttt tcgttgctca ccgcggactg cgcgccgctg tttggcaggc
     2101 tcatgccgag ggcttcgatg gcgctggcca tcgtgttggc ggtgtacatg cctccgcagg
     2161 agccttcacc tggaatggcg tgctcctcga tgtcttgcag ctccttgtca ttgatctggc
     2221 agttggcgtg cttgccgatg gcctcaaaga ccgtgacgac atccgcgtcc ttcttctccg
     2281 ggccgacgca gccggggagg atggtgccgc cataaacaaa gacgctcggg cggttcatgc
     2341 ggcccatcgc catgatgcag ccgggcatgt ttttgtcaca gccgccgatg gcgacgtagc
     2401 cgtccatgcc ttcgcagccg acgacggtct cgatcgagtc cgcgatgact tcgcgggaga
     2461 cgagcgagta tttcatgccc tccgtgccca tcgagatgcc gtcggagatg gtgatggtgt
     2521 taaagatcac cgatttgccg cccgcagcat cgacgccctt cgcggcttct ttggcgagac
     2581 ggtcaatgtg catgttgcac ggcgtcacca tgctccaggt gctggcgatg ccgatcttgt
     2641 tctttttgaa gtcctcgcgc ttgaagccca cagcgtgcag catcgcgcgg ctggcggcgc
     2701 gttccacgcc atcgacgacg gcggcgctgt gtttgcggtg cagggaggcg ttggaggcgg
     2761 ctttcttgga catggtgtgg aatgagaacg cgcaaggtgc ctgaagcccc tgcgctcgca
     2821 agcagccttt gccaggtgcg ccgaactttg gttgaaatgc cccgcatggc gcgggcgttg
     2881 ccacaagaaa gcatgcacac acgccgtcac ttcctcacgc gctccggctt tggcctcggc
     2941 agcctcgcgc tggccggggt gctcaatgac atgggcctga cccgcgcgaa tgcggaaaat
     3001 ctctcgccac tggctccgcg acgaccgcat tttccaggca cggcgaagcg cgtcattcac
     3061 cttttcatga atggcggccc ctcgcagata gacaccttcg atccgaagcc gctgctcaag
     3121 aaatttcatg gcaagtcgct gccctccgcg aagaatctgg cgaaggaccg ccgccttggc
     3181 ggcgcggcgc tggcctcgcc tttcgagttc aaaaagcacg gccagtccgg catggagatc
     3241 agcgagcttt tcccgcatgt ggcgaggcac gcggacgact tgtgcgtgat ccgctccatg
     3301 ttcagcgacg tgccgaacca cgagcccggc ctcatgatca tgaacacggg caatttcacc
     3361 ctgccgcgcc cgagcgtcgg ctcatgggcg ctctatggcc tgggcacgga gaatcaaagc
     3421 ctgcccggct ttgtggtcat gtgcccgaac ggcctgccga cggcgcaaag cgcgaactgg
     3481 cgcaacgcct tcctgcccgg catctaccag ggcacgcaca ttgacaccaa ggagaccacg
     3541 ccggaccagc tcatcgccaa cctgcgcaac gacttcatcc tgcccggtca gcagcgcaag
     3601 caactcggct tcatccagaa gctcaatgaa atgcaccgcg cgcaacgcgc gggtgatccg
     3661 cagctcgagg cgcgcattca gtccctggag ctggccttcc gcatgcagac cgaggccacg
     3721 gaggccttcg atgtggagca ggagccggcg aacatccgcg agatgtatgg cgacacgctt
     3781 cagg
//
LOCUS       DAOMYJ010000355         3773 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Prosthecobacter sp. isolate AMR_MDS_5629
            NODE_8696_length_3773_cov_24.413663, whole genome shotgun sequence.
ACCESSION   DAOMYJ010000355 DAOMYJ010000000
VERSION     DAOMYJ010000355.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867850
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prosthecobacter sp. (wastewater metagenome)
  ORGANISM  Prosthecobacter sp.
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Verrucomicrobiaceae; Prosthecobacter.
REFERENCE   1  (bases 1 to 3773)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3773)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.30
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:34:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,742
            CDSs (total)                      :: 4,684
            Genes (coding)                    :: 4,676
            CDSs (with protein)               :: 4,676
            Genes (RNA)                       :: 58
            tRNAs                             :: 55
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3773
                     /organism="Prosthecobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8696_length_3773_cov_24.413663"
                     /isolate="AMR_MDS_5629"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1965333"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..201
                     /locus_tag="PLP58_21615"
     CDS             <1..201
                     /locus_tag="PLP58_21615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ11194.1"
                     /translation="AWLVLRARRARALITGAGLMFAALVAVFLVFQSALVWCPPTMPV
                     FALASGALIARLFGRAAAVDAP"
     gene            322..>3773
                     /gene="gltB"
                     /locus_tag="PLP58_21620"
     CDS             322..>3773
                     /gene="gltB"
                     /locus_tag="PLP58_21620"
                     /EC_number="1.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009965039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0015930 - glutamate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0006537 - glutamate biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase large subunit"
                     /protein_id="HRJ11195.1"
                     /translation="MKHPHYDLDIPNEGSLHLIENEKDACGVGFVANIHGKRSRDILD
                     MAICGVCSVQHRGAVDADRVTGDGSGVLTQIPYDVLLPEVEKLGHALDHPMDLAVGVF
                     FLPNEPMEVMRIHLLTESILRYRGIKALGWRDVPVNPNELGEKARRTMPLIQHLFLAR
                     PEDMGDHEFERQLYLVRRELAIKTREQKLTNFYVASLSHRTIVYKALLLPSSLQKFYT
                     DLQQDEYDTSLALFHQRFSTNTFPTWALSHPFRMLAHNGEINTVRGNRNWLASRTSDF
                     ESELWAGDEHLLKRLLDVNTSDSASLDQALELLVLSGRSLTHALAMLVPSAYGIDPGT
                     SPELKAFYEFHSCFSEPWDGPAALVMTDGTRVAAGLDRNGLRPSRWKLTEDGIFALGS
                     EVGIIHLDDSKVIRKGRLAPGEILEVDTSKGRVRFNAEIKAELAARQPYGEWLKNRTQ
                     LTALAPNPPKDDLDILTLSQKQAAFGWTKEELDFSLIPMLQKGEESVYSMGDDVALSI
                     LSTRPRLLPSYFKQTFAQVTNPPIDPIRERAVMSLDVVLGWQRNLLDESPDHAKVVHL
                     TSPFLFENELEKLKSLPDYCCRTLDMTWPVSEGPAGLKAAIVRLCHEAGRAVQDDVSI
                     LILSDRAVDHDRVAVPVLLAVGAVHQHLNRKQVRMRLSIVADTAEARDTHQMATLFGF
                     GASAVCPYLAFDTIHEALEADKTARKPQLEGIEYTKALTNFRKALEKGVLKIMSKMGI
                     SVLSSYTGAQIFEAVGIGKEVIDFCFTGTPSQIGGIGFADIAEESLARHALGFSQPVP
                     EGAGAEEAKPGPVDLGDPGFYRPRQKGEMHAITGPVIKNFHTFVKTGAPEDYQKYVSS
                     QLENTPVALKDLLEFVPSPDGPIPIEEVEPIEDIRVRFTTAAMSLGAISPEAHEALAI
                     AMNRIGGKSDSGEGGEDPKRFRPYDNGDWANSKIKQVASGRFGVTAEYLSSAWEIEIK
                     MAQGAKPGEGGQLPAMKVNHLIARLRNTTPGVMLISPPPHHDIYSIEDLAQLIHDLKE
                     VNSRARVCVKLVAESGVGTIAAGVAKANADIILISGHDGGTGASPLSSIKYAGLPWEL
                     GLAETQQVLMLNSLRERVTLRTDGGLRNGRDIAIAAMLGAEEFNFGTIALIALGCVYV
                     RQCHL"
BASE COUNT          680 a         1420 c         1055 g          618 t
ORIGIN      
        1 gcgtggctgg tcctgcgcgc gcgtcgcgcg cgcgcgttga tcacaggcgc ggggctgatg
       61 tttgccgcgc tggtggccgt ctttctggtc tttcagtccg ccctggtctg gtgcccgccg
      121 acgatgccgg tcttcgctct ggcttcaggg gcgctcatcg cgcggctctt tggccgcgcc
      181 gcagccgtgg atgccccctg agcgcggtct ggcgcggccc ctgtttctgg cattctcctt
      241 gcttgccaat caagtgcaac gctccaaaat acccgcccct ccgaccggac ggcagtcgtc
      301 cgcagagggg cttcttgccc catgaaacat ccccattacg acctggacat ccccaatgaa
      361 ggcagcctcc atctcattga aaacgagaag gatgcctgcg gcgtcggctt tgtggcgaac
      421 attcatggca aacgcagccg cgacattttg gacatggcca tctgcggcgt gtgcagcgtc
      481 cagcaccgcg gcgcggtgga tgccgaccgc gtgaccgggg acggctcagg tgtgctcacc
      541 cagattccct atgacgtgct gctgcccgag gtggaaaaac tcggccatgc cctggatcat
      601 ccgatggacc tcgctgtcgg cgttttcttc ctgccgaacg agccgatgga ggtcatgcgc
      661 atccacctgc tcaccgagag catcctgcgc taccgcggca tcaaggcgct cggctggcgt
      721 gatgtgccgg ttaacccgaa cgagctgggt gaaaaagccc gccgcaccat gccgctcatc
      781 cagcacctct tcctggcgcg gccggaggac atgggggacc acgaattcga gcgccagctc
      841 tacctcgtgc gccgcgagct ggccattaag acgcgcgagc agaagctgac gaacttttac
      901 gtcgcctccc tttcgcaccg caccatcgtc tataaagcgc tgctcctgcc ctcctccctc
      961 cagaagtttt acaccgacct ccagcaggac gagtacgaca cctcgctggc gcttttccac
     1021 cagcgctttt ccacaaacac cttccccacc tgggcgctca gccacccctt ccgcatgctg
     1081 gcgcacaatg gcgagatcaa caccgtgcgc ggcaaccgca actggctcgc cagccgcacc
     1141 agcgacttcg agagcgaact ctgggctggc gatgagcacc tgctcaaacg cctgctcgat
     1201 gtcaacacct ccgactccgc aagcctggac caggcgctcg agctgctcgt gctcagcggc
     1261 cgcagtctca cccatgccct ggccatgctc gtgcccagcg cctacggcat tgaccccggc
     1321 acctcccccg agctgaaggc cttctacgag ttccactcct gcttcagcga gccctgggac
     1381 ggccccgccg ccctggtcat gaccgacggc acccgcgtcg ccgccggcct cgatcgcaac
     1441 ggcctgcgcc ccagccgctg gaagctcacc gaggacggca tcttcgccct cggctccgag
     1501 gtcggcatca tccacctcga cgactcgaag gtcatccgca aaggccgcct ggcccccggc
     1561 gagatcctgg aggtggacac cagcaagggc cgtgtgcgct tcaatgcgga gatcaaggcc
     1621 gaactcgccg cccgccagcc ctacggcgag tggctcaaaa accgcaccca gctcaccgcc
     1681 ctggcgccca acccgcccaa ggacgatctc gacatcctca ccctctccca gaagcaggcc
     1741 gcctttggct ggaccaagga ggagctggat ttctcactca tccccatgct ccagaagggc
     1801 gaggaaagcg tctactccat gggggatgac gtggccctct ccatcctttc cacacgtccg
     1861 cgcctcctgc ccagctattt caagcagacc ttcgcccagg tcaccaaccc gcccattgac
     1921 cccatccgcg agcgcgccgt catgagcctg gatgtcgtgc tcggctggca gcggaacttg
     1981 ctcgatgagt caccggacca cgccaaagtc gtccacctca ccagcccctt cctcttcgaa
     2041 aacgagctcg aaaaactcaa gagcctgccg gattactgct gccgcacgct cgacatgacc
     2101 tggcctgtct ccgaaggccc cgccgggctg aaggccgcca tcgtccgcct ttgccacgag
     2161 gcaggccgcg ccgtgcagga tgacgtgagc atcctcatcc tcagcgaccg cgccgtggat
     2221 cacgaccgcg tcgccgtgcc ggtgttgctc gccgtcggtg ccgtgcatca gcacctcaat
     2281 cgcaagcagg tgcgcatgcg cctgagcatc gtcgccgaca ccgccgaggc gcgcgacacg
     2341 caccagatgg ccaccctttt cggcttcggc gcctccgccg tctgccccta tctggccttc
     2401 gacaccatcc acgaggcgct ggaggcggac aaaaccgccc gcaagccgca gcttgagggc
     2461 atcgagtaca ccaaggcgct cacgaacttc cgcaaagccc tcgaaaaagg cgtgctcaag
     2521 atcatgagca agatgggcat ctccgtactc agcagctaca ccggcgcgca gattttcgag
     2581 gcagtcggca ttggcaaaga agtcattgat ttctgcttca ccggcacccc ctcccagatc
     2641 ggaggcatcg gcttcgccga catcgccgag gagtccctgg cccggcacgc cctcggtttc
     2701 tcccagcccg tcccggaagg cgcgggcgcc gaggaggcaa aacccggccc tgtggacctg
     2761 ggcgaccccg gcttctaccg tccgcgccag aaaggcgaga tgcacgccat caccggcccg
     2821 gtcatcaaaa acttccacac cttcgtcaag accggcgcgc cagaggacta ccaaaagtac
     2881 gtctcctcgc agcttgagaa cacacccgtg gccttgaagg acctgctgga gttcgtcccc
     2941 agccctgacg gccccattcc catcgaggaa gtggagccca tcgaggacat ccgtgtgcgc
     3001 ttcaccaccg ccgccatgtc cctcggcgcc atctcccccg aggcgcacga ggcgctcgcc
     3061 atcgccatga accgcatcgg cggcaagtcc gactccggcg agggtggcga ggaccccaag
     3121 cgcttccgcc cctatgacaa cggcgactgg gcgaactcga agatcaagca ggtggcctcc
     3181 ggccgcttcg gcgtcaccgc cgaatacctc agcagcgcct gggagatcga gatcaaaatg
     3241 gcccagggcg ccaagcccgg cgagggcggc cagctcccag ccatgaaggt gaaccacctc
     3301 atcgcccgcc tgcgcaacac cacccccggc gtcatgctca tcagcccgcc gccgcaccat
     3361 gacatctact ccatcgagga tctggcccag ctcatccacg acctcaaaga ggtcaactcc
     3421 cgcgcccgcg tctgcgtgaa gctcgtcgcc gaatccggcg tcggcaccat cgccgccggt
     3481 gtggccaagg ccaacgccga catcatcctc atctccggcc acgacggcgg cacgggcgcc
     3541 agcccgctct cctccatcaa atacgccggc ctgccctggg agctcggcct cgccgagacc
     3601 cagcaggtgc tcatgctcaa cagcctccgc gagcgcgtca ccctgcgcac cgacggcggc
     3661 ctgcgcaacg ggcgcgacat cgccatcgcc gccatgctgg gcgccgagga gttcaacttc
     3721 ggcaccatcg ccctcatcgc cctcggctgc gtctatgtgc gccagtgcca cct
//
LOCUS       DAOMYJ010000356         3750 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Prosthecobacter sp. isolate AMR_MDS_5629
            NODE_8765_length_3750_cov_20.801083, whole genome shotgun sequence.
ACCESSION   DAOMYJ010000356 DAOMYJ010000000
VERSION     DAOMYJ010000356.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867850
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prosthecobacter sp. (wastewater metagenome)
  ORGANISM  Prosthecobacter sp.
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Verrucomicrobiaceae; Prosthecobacter.
REFERENCE   1  (bases 1 to 3750)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3750)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.30
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:34:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,742
            CDSs (total)                      :: 4,684
            Genes (coding)                    :: 4,676
            CDSs (with protein)               :: 4,676
            Genes (RNA)                       :: 58
            tRNAs                             :: 55
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3750
                     /organism="Prosthecobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8765_length_3750_cov_20.801083"
                     /isolate="AMR_MDS_5629"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1965333"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            296..1315
                     /gene="pheS"
                     /locus_tag="PLP58_21625"
     CDS             296..1315
                     /gene="pheS"
                     /locus_tag="PLP58_21625"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009960238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HRJ11196.1"
                     /translation="MLSELDSLQSEALAALESLQDEAAMEAFRIDYLGKKGRLTAISA
                     RMREVPPEQKKDVGAKLNEVREAITGGLDSRLVALQAAKDAASVAGIDLTLPGRPGMG
                     AGSLHPLTQIRDLAVRTLRRMGFTLADGPEIETEWHCFDALNTPADHPARNESDTFYM
                     PDGKLMRTHTSTVQIRTMETVKELPVRIIAPGAAYRRDEIDATHLSVFNQIEALYVDR
                     EVSLADLKGTLEFFFREVFGPKTAVRFRPHFFPFTEPSFEIDVKLEAKGREDRWIEIA
                     GCGMVDPAVFAAVCRTRGDLLFDPGAVTGFAFGMGLDRLAMILHGITDIRHLIENDAR
                     FLRQF"
     gene            1431..>3750
                     /gene="pheT"
                     /locus_tag="PLP58_21630"
     CDS             1431..>3750
                     /gene="pheT"
                     /locus_tag="PLP58_21630"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: protein motif:HMM:TIGR00472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HRJ11197.1"
                     /translation="MQVSLSWLSTHLDLSSYTTAQLSDLLTFAGVEVEGIEEKGVASD
                     KVVVAQILSFEQHPNADKLSVCQVDDGSGAPRQIVCGAKNFKAGDKVPLALPGAQLPG
                     GFEIKEGKLRGVVSGGMMCSGKELGIGEDHGGLLILDAGAPVGKPFKELVEPDTIFDL
                     EITPNRPDLLSHLGLARELAALTGLPLKQARDHTASSTKTRHAKADEVRLDAPDGCPL
                     YTARVIKGVKVGPSPDWLKARLESIGLRPINNIVDITNYVLMEMGQPLHAFDLAKLEG
                     GIVVRWAAEGETIRALDEKDYALTGTDLVIADSKKPVAIAGVMGGADTGVTEGTTDIL
                     LEAAYFTPSIVRRGARRLGISSDSSYRFERGADPMQVRGASDLAAHHIVTLAGGKAED
                     EIVIAGEAPALTGEVELDEARAHRLLGMPELSVDEAHGILEKLGLVKQAATKEKSRWQ
                     IPSYRLDLQRGVDLVEEVARVVGLDRVPSRFTAVMAQGDATDRQYDHAMQLRQALAAR
                     GFHEAQTLRLIAASQLGDALGAGDPAASGAALKNPLSEDHTHLRPSIVPGLLATAELN
                     IRQGAQRLRFFEAGRVFLKMPNGQVREEERLAVLLSGPAAESSWHEKEPRAVDVFDLR
                     GLIEALPGVAALEVKKLDDTGTLLLRHELRAGNRVLGWLAQVHPARARAMDARHPVYA
                     AELLLSALRQGSGGPAKFEELPRFPAVTRDVAFELPLDFPNARVRAFFTGVKEPLFMG
                     AEIFDVFTDSAGMKIAAGHVGHAHRVADGLGLH"
BASE COUNT          644 a         1105 c         1338 g          663 t
ORIGIN      
        1 ttccgtggca aaagcatgtg cttccgtggc aaaagcatgt gcttccgtgg caaaagcatg
       61 tgcttctatg gcaaaccctt gagggttttt gccctggccc ttgctcccca ctccgcgtca
      121 gcggtctttc gctggacggg ccactgcgtg tccctgccag agtcaacaac cccaaccgcc
      181 gcgccgccgg gctgagaggc gcggcggttt cggaggcagc gttgacttgc gcggcggatg
      241 atccgggcca tgatccacgc cctttcgcgc cgggcgggcg cgcgctatcc cgatcatgct
      301 ttccgaactc gactccctcc aatccgaggc cctggcggct ctcgaatccc tccaagacga
      361 agcggcgatg gaggccttcc gcattgatta cctggggaaa aaaggccgcc tcaccgccat
      421 cagcgcgcgc atgagggaag tgccgccgga gcagaagaag gatgtggggg cgaagctcaa
      481 cgaggtgagg gaggccatca ccggcgggct ggacagccgg ctggtggcgc tgcaggcggc
      541 gaaggatgcg gcctcggtgg cgggtattga tcttacgctg cctggcaggc cgggcatggg
      601 cgcgggcagc ctgcatccgc tgacgcagat ccgggatctg gcggtgcgca cgctgcggcg
      661 catgggcttc acgctggcgg acgggccgga gatcgagacg gagtggcatt gcttcgacgc
      721 gctgaacacg ccggcggacc atccggcgcg caatgagagc gacacgtttt acatgccgga
      781 tggaaagctc atgcgcacgc acacttcgac ggtgcagatc cgcacgatgg agacggtgaa
      841 ggagctgccg gtgcgcatca tcgcccccgg cgcggcctac cggcgggatg agattgacgc
      901 cacgcacctg agtgtcttca accagatcga ggcgctgtat gtggacaggg aggtgagcct
      961 ggcggatctg aagggcacgc tggagttttt cttccgcgag gtcttcgggc cgaagacggc
     1021 ggtgcgtttc cgcccgcatt tcttcccctt cacggagccg agcttcgaga ttgatgtgaa
     1081 gctggaggcg aagggcaggg aggaccggtg gatcgagatc gcgggctgcg gcatggtgga
     1141 cccggcggtg ttcgcggcgg tgtgcaggac gcgcggggat ttgctcttcg atcccggggc
     1201 ggtgacgggc tttgcttttg gcatgggcct ggaccgcctg gcgatgatcc tgcacggcat
     1261 cacggacatc cgccacctca ttgaaaatga cgcgcgtttc ctgcggcagt tttgacgcgg
     1321 gtggaaacca cgaatggaca cgaaacaaca cgaacttttc caaaccctct gacacgatca
     1381 ttagtgcgaa ttagtgagca ttagtggtta gacgcttttt ttgacatccc atgcaagtct
     1441 ccctctcctg gctcagcact catctcgacc tctcctctta cacgacggcg cagctttccg
     1501 atctcctgac cttcgcgggt gtggaggtgg agggcattga ggagaaaggc gtggcgtcgg
     1561 acaaggtcgt ggtggcgcaa atcctttcct tcgagcagca cccaaacgcg gacaagctca
     1621 gcgtctgcca ggtggacgac ggatcgggcg cgccgcggca gattgtctgc ggggcgaaga
     1681 atttcaaagc gggcgacaag gtgccgctgg cgctgccggg cgcgcagttg ccgggcggat
     1741 ttgagatcaa ggaaggcaag ctgcgcgggg tggtctccgg cggcatgatg tgcagcggca
     1801 aggagctggg catcggcgag gaccatggcg ggctgctcat tctggatgcc ggcgcgccgg
     1861 tgggcaaacc tttcaaggag ctggtggagc cggacacgat ttttgatctg gagatcacgc
     1921 cgaaccgccc ggatttgctc agccacctgg gcctggcgcg cgagctggcg gctttgaccg
     1981 gcctgcctct gaagcaggcg cgggatcaca cggcatcatc cacgaagacc cgccatgcga
     2041 aggcggacga ggtgcggctg gatgcgccgg atggatgccc gctttacacg gcgcgcgtca
     2101 tcaagggcgt gaaggtgggg ccgagcccgg actggctcaa ggctcggctg gaaagcatcg
     2161 gcctgcgccc gatcaacaac atcgtggaca tcacgaacta tgtgctcatg gagatggggc
     2221 agccgctgca tgcttttgat ctggcgaagc tggagggcgg catcgtggtg cgctgggcgg
     2281 cggagggtga gacgatccgc gctttggatg aaaaagatta cgcgctcact ggcacggacc
     2341 tggtgatcgc ggactcgaag aaaccggtgg ccatcgcggg tgtcatgggt ggcgcggaca
     2401 cgggcgtgac ggagggcacg acggacatcc tgctggaggc ggcgtatttc acgccttcca
     2461 tcgtgcgccg tggcgcgcgc aggctgggca tcagctcgga ctccagctac cgcttcgagc
     2521 gcggcgctga tccgatgcaa gtgcgcggcg cgagtgatct ggccgcgcat cacatcgtga
     2581 cgctcgcggg tggcaaagcg gaggatgaga tcgtcatcgc gggcgaggct ccggctttga
     2641 cgggcgaggt ggagctggac gaggcgcgcg cgcatcgcct gctcggcatg cctgagctga
     2701 gcgtggacga ggcgcatggc attctggaaa agctcggcct ggtgaagcag gcggccacga
     2761 aggaaaagag ccgctggcag attcccagct accgcctgga tttgcagcgc ggggtggact
     2821 tggtggagga agtcgcgcgc gtggtcgggc tggatcgtgt gccctcccgt ttcactgccg
     2881 tcatggccca gggtgatgcc acggaccgcc agtatgatca tgcgatgcaa ctgcgccagg
     2941 cactcgccgc gcgcggtttc catgaggcgc agaccttgcg cctcatcgcc gccagccagc
     3001 tcggtgacgc tctcggcgcg ggcgaccctg ccgcatccgg cgcggctttg aaaaatccgc
     3061 tcagcgagga ccacacgcac ctgcgcccga gcatcgtgcc gggtctgctg gccacggctg
     3121 agctgaacat ccgccagggc gcgcagaggc tgcgcttctt cgaggcgggg cgcgtgtttt
     3181 tgaaaatgcc caacggccag gtgcgcgagg aggagcggct ggcagtgctg ctcagcggtc
     3241 cggcggcgga gagttcctgg catgagaagg agccgcgcgc cgtggatgtc tttgatctgc
     3301 gcggtctgat cgaggcgctg ccgggtgtcg cggccctgga ggtgaagaag ctggatgaca
     3361 caggcactct gctgctgcgg catgaactgc gcgcgggcaa ccgcgtgctc ggctggctgg
     3421 cccaggtgca tccggcgcgc gcgcgcgcca tggacgcgcg gcatccggtc tatgcggccg
     3481 aactgctgct cagcgcgctg cgccagggca gcgggggacc ggcgaagttc gaggaattgc
     3541 cgcgcttccc tgcggtgact cgggacgtgg ccttcgagct gccgctcgac tttccgaatg
     3601 ccagggtgcg cgcgtttttc acaggcgtga aagagccgct gttcatgggc gcggagatct
     3661 tcgatgtctt caccgacagc gccgggatga aaatcgccgc cgggcatgta ggtcacgcgc
     3721 atcgggtcgc tgatggcctt ggcctccatc
//
LOCUS       DAOMYJ010000357         3715 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Prosthecobacter sp. isolate AMR_MDS_5629
            NODE_8891_length_3715_cov_22.387705, whole genome shotgun sequence.
ACCESSION   DAOMYJ010000357 DAOMYJ010000000
VERSION     DAOMYJ010000357.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867850
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prosthecobacter sp. (wastewater metagenome)
  ORGANISM  Prosthecobacter sp.
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Verrucomicrobiaceae; Prosthecobacter.
REFERENCE   1  (bases 1 to 3715)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3715)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.30
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:34:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,742
            CDSs (total)                      :: 4,684
            Genes (coding)                    :: 4,676
            CDSs (with protein)               :: 4,676
            Genes (RNA)                       :: 58
            tRNAs                             :: 55
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3715
                     /organism="Prosthecobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8891_length_3715_cov_22.387705"
                     /isolate="AMR_MDS_5629"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1965333"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            113..982
                     /locus_tag="PLP58_21635"
     CDS             113..982
                     /locus_tag="PLP58_21635"
                     /inference="COORDINATES: protein motif:HMM:NF014477.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0017013 - protein flavinylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HRJ11198.1"
                     /translation="MMHCFEEHAMATSFQVIIAQEDVDEAYAAHAAEAVFTEIRRLED
                     ELSRFRPGSDIWRLSRLRAGESARVSLATWDCLSLAKAMHEETGGAFDITIGPLMHLW
                     RDENDNLRQPPPEALERARQSVGSHLFDLHEDDGLHVSVRADHMVFDLGGLGKGYALD
                     QAAVLLGEWSITRAFLNAGDSTLLALGSPPGEDAWEVTLAEGARFLTLRDRSLSGSGF
                     MVKGAHIMDPRTLQPLSVREKRGYALAPAAALSDALSTAFMILDPEAIHSLCARNPGV
                     EWLEVWETLEGGA"
     gene            1035..2339
                     /locus_tag="PLP58_21640"
     CDS             1035..2339
                     /locus_tag="PLP58_21640"
                     /inference="COORDINATES: protein motif:HMM:NF019262.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ11199.1"
                     /translation="MLRQALILLLLTAPAHADTRAAMRVLRDECVGCHKPGKAKGGLL
                     LTTHEKMMKGGDSGPAVVPGKPADSPLYHLVLADADPHMPPKKQLDTAALKALESWVK
                     AGAPWDARVFDEPPAPRPVKLSSMPETWQPVLALALSPDGKNLAVARGGSVILVDMAA
                     RERPVLGRLEGHAGPVQSLAWTPDGARLVTGGYRDIRLWDPAALQQTGGITTALIGMV
                     TALAATDTHVFAADGETGGAGFLHQFDFHGKLTATWKAHDDNILALRLSAKGDRLLSG
                     GADRMARLWDTSTRALVAFYEGHTNHVHSVAFDHDASRIATAGADREVKVWDVPTREQ
                     DASLGDKKNAYVALSWTPDGKALAAITDKGHGSVYTELKKHDGAQRSDTAKERRLESA
                     GAPLTTVVITADAKTVIAGSFDGRLHTWDAATGKPGSGIALE"
     gene            complement(2388..3413)
                     /locus_tag="PLP58_21645"
     CDS             complement(2388..3413)
                     /locus_tag="PLP58_21645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose oxidase"
                     /protein_id="HRJ11200.1"
                     /translation="MKRHALFLLPALALYGAEISWSPLPALPDAEGFAGSFAGVSGGA
                     LLVAGGANFPDAPPWRGGTKTWYDGIFALDRPDGAWRVAGRLPRPNGYGVSLSIPEGL
                     VCIGGGDAAAHFREAFVLRVFKEGLVNEPLPPLPRPCAYMAGAVLDGVLHVCGGTEKP
                     DATEAMPAFWALDMREEKPAWRELPACPGPARILAVMGAHDGALYLFSGAALKAGADG
                     RPEREWLRDAWKYTAASRTWKRIADLPRVAVAAPGPAPVVEGRLLVVGGDDGANAAFE
                     PKDEHPGFPRGILAYDPARDEWSGAGEAPFSLVTTALVEWQGRLVIPGGEARPGKRSP
                     QVWSGGW"
     gene            3514..>3715
                     /locus_tag="PLP58_21650"
     CDS             3514..>3715
                     /locus_tag="PLP58_21650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ11201.1"
                     /translation="MLEFFRRHRGAFLITVTVIIIISFSVWGGWKSGRDSIMAQPTDP
                     AFEVYGRTYTVAEAQRLSRRLNV"
BASE COUNT          635 a         1464 c         1095 g          521 t
ORIGIN      
        1 gcgaaccact cacccccgga gccggcggcg ccacgctgac acccgcgggc acgctttcca
       61 ccccgccggt cttctccccc gaactgccgc ccctgccggg catgccgcga ccatgatgca
      121 ctgcttcgag gagcatgcga tggccaccag cttccaggtc atcatcgccc aggaggatgt
      181 ggacgaagcc tacgccgccc atgccgccga ggctgtcttc acggaaatcc gccgcctgga
      241 ggacgaactc agccgcttcc gccccggcag cgacatctgg cgcctctcac ggctccgcgc
      301 cggagagagc gcccgcgtct ccctcgccac ctgggactgc ctgagcctgg ccaaggcgat
      361 gcacgaggag acaggcggcg cgtttgacat caccatcggc ccgctgatgc acctctggcg
      421 ggatgagaat gacaacctcc gccagccgcc gccggaagct ctggagcgcg cgcgccagtc
      481 ggtgggatcg catctcttcg accttcacga ggacgacggc ctgcatgtgt cggtgcgcgc
      541 ggatcacatg gtcttcgacc tcggcggact gggcaaaggc tacgcgctgg atcaagccgc
      601 cgtgctgctc ggcgaatgga gcatcacgcg cgctttcctc aatgccggag acagcactct
      661 gctggcgctt ggcagccctc ccggagagga tgcatgggaa gtcaccctgg cagaaggcgc
      721 gcgcttcttg acactgcgcg accgctccct gagcggcagc ggcttcatgg tcaaaggcgc
      781 gcacatcatg gacccgcgca cactccagcc cctcagcgtg cgtgaaaaac gcggctacgc
      841 cctggcaccc gccgccgccc tctccgatgc gttatcaacc gccttcatga tcctcgatcc
      901 ggaggccatc cactccctct gcgcgcgcaa tcccggcgtc gaatggctgg aggtttggga
      961 aacgctggaa ggaggcgcat gagtgaaaca cacgttccgc aatccccgcc ccgcggccgc
     1021 cgtattagcc cctgatgctc cgccaagccc tgatcctcct gctcctcacc gccccagccc
     1081 atgccgacac ccgggccgcc atgcgtgttc tgcgtgacga gtgcgtcggc tgccacaagc
     1141 cgggcaaagc caagggcggc ctgctgctca ccacccacga gaagatgatg aaaggcggcg
     1201 acagcggccc cgccgtcgtc cccggcaaac ccgccgacag ccctctctac cacctcgtcc
     1261 tcgccgatgc cgatcctcac atgccgccga agaagcaact cgacaccgcc gccttaaagg
     1321 ccctcgaaag ctgggtcaaa gcaggtgctc cctgggatgc cagggtcttc gacgagccgc
     1381 ccgcgccgcg ccccgtgaag ctctccagca tgcctgagac atggcagccc gtgctcgcgc
     1441 tggcgctttc accggatggc aaaaatctgg ccgtcgcgcg cggcggcagc gtcatcctgg
     1501 tggacatggc ggccagggag cgccccgtac tcggccgcct cgaagggcac gcaggacccg
     1561 tgcaatcgct cgcctggaca cccgacggcg ctcgccttgt caccggcggc taccgtgaca
     1621 tccgcctctg ggaccctgcg gccctccagc agaccggcgg catcaccacc gcgctcatcg
     1681 gcatggtcac cgcccttgcc gccactgaca cgcatgtctt tgccgccgat ggcgagactg
     1741 gcggcgccgg tttcctccat cagttcgatt ttcacggaaa gctcactgcc acatggaaag
     1801 cgcacgatga caacatcctc gccctccgcc tctccgccaa aggagaccgc ctcctcagtg
     1861 gcggcgcgga caggatggca cgcctctggg acacctcgac gcgcgcgctc gtcgcctttt
     1921 atgaaggcca caccaaccac gtccactccg tggccttcga ccatgacgca agccgcatcg
     1981 cgaccgccgg agccgaccgc gaggtcaaag tctgggacgt gcccacccgc gagcaggacg
     2041 cctccctcgg cgacaaaaag aacgcctatg tcgccctctc ctggacaccc gacggcaagg
     2101 cgctcgccgc catcactgac aaaggccacg gcagcgtcta taccgagttg aaaaagcacg
     2161 acggagcgca gcgcagcgac accgccaaag agcgcaggct ggaaagcgcc ggcgcgcccc
     2221 tcaccaccgt cgtcatcacc gccgacgcca aaaccgtcat cgccggctcc ttcgacggca
     2281 ggctccacac ctgggacgcc gccaccggca agcccggcag cggcatcgcc ctggagtgaa
     2341 gcccccgccc gcagagggaa aacgccccgg cgcggacacc cgccgcctca ccagcccccg
     2401 ctccacacct gcggagatct cttgcccggc ctcgcctccc cgccggggat gaccagccgc
     2461 ccctgccact ccaccagcgc ggtcgtcacc agggaaaacg gcgcctcccc cgccccgctc
     2521 cactcgtccc tggcgggatc ataggccagg atcccgcgcg gaaagccggg atgctcatcc
     2581 tttggctcaa aggccgcgtt cgctccatca tccccgccga ccaccagcag ccgcccctcc
     2641 accacgggcg cgggtccagg cgcggccacc gccacgcgcg gcaggtcggc gatgcgcttc
     2701 catgtccgcg aggcggcggt gtatttccac gcgtcacgca gccactcacg ctccggcctg
     2761 ccatccgctc ccgccttcaa ggcggctccg ctgaagagat acagcgcccc gtcatgagcg
     2821 cccatcaccg ccaggatgcg cgccgggccg gggcacgcgg gcagctcccg ccacgcgggt
     2881 ttctcctccc gcatgtccag cgcccagaac gcgggcatcg cctccgtggc gtccggcttc
     2941 tccgtgccgc cgcacacatg cagcaccccg tccagcaccg cccccgccat gtaggcgcac
     3001 gggcgcggca gcggcggcag cggctcgttc accagaccct ccttgaagac ccgcagcaca
     3061 aaagcctccc ggaaatgcgc ggcggcgtcc ccgcccccga tgcacaccag gccctcgggg
     3121 atcgagagcg acacaccata gccgttcggg cgcggcagtc tgcccgccac ccgccatgcg
     3181 ccatccggcc ggtccagggc aaagatgccg tcataccagg tcttcgtgcc cccgcgccag
     3241 ggtggcgcgt cggggaagtt cgccccgccg gccaccagca gcgcgccgcc gctcaccccc
     3301 gcgaaggagc cggcaaagcc ctccgcgtcc ggcagcgccg gcagggggct ccaggaaatc
     3361 tccgcgccgt aaagcgcgag ggcgggcagc aggaatagcg cgtgtcgttt catcagccca
     3421 gcataccgcg cacccgccgc tggtcatgga ttttccggtt gccgccagag ggagggcggc
     3481 ttacttggcg gcccttttac ccagccgcac gccatgctcg agttcttccg ccgccaccga
     3541 ggcgccttcc tcatcactgt caccgtcatc atcatcatca gtttctccgt ctggggcggc
     3601 tggaagtcgg ggcgtgacag catcatggcc cagcccaccg accccgcctt cgaggtctat
     3661 ggccgcacct acaccgtggc cgaggcccag cgcctcagcc gccgcctcaa cgtga
//
LOCUS       DAOMYJ010000358         3681 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Prosthecobacter sp. isolate AMR_MDS_5629
            NODE_8994_length_3681_cov_16.327910, whole genome shotgun sequence.
ACCESSION   DAOMYJ010000358 DAOMYJ010000000
VERSION     DAOMYJ010000358.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867850
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prosthecobacter sp. (wastewater metagenome)
  ORGANISM  Prosthecobacter sp.
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Verrucomicrobiaceae; Prosthecobacter.
REFERENCE   1  (bases 1 to 3681)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3681)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.30
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:34:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,742
            CDSs (total)                      :: 4,684
            Genes (coding)                    :: 4,676
            CDSs (with protein)               :: 4,676
            Genes (RNA)                       :: 58
            tRNAs                             :: 55
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3681
                     /organism="Prosthecobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8994_length_3681_cov_16.327910"
                     /isolate="AMR_MDS_5629"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1965333"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1037)
                     /locus_tag="PLP58_21655"
     CDS             complement(<1..1037)
                     /locus_tag="PLP58_21655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ11202.1"
                     /translation="MAVPTSNFEIRSSRFSPRPSGAALVWVLLLTGFMAVVAIAFLGS
                     SSIQNTNARLDQAETLTDELMLSARQEVLAKLREAFVVEKTGAFTGHVAATPGLLEVR
                     RYDVPWNRGGHTGAAAFTGTKENPAPFSQPFSATYQGEPANPRHIPLFSWKAFAPRLK
                     HLTVQGGTPSRLNPDYNPALSFNLNTPRNPFAPGACLLSGVPEDAEALVRERTGTRWS
                     DDSFRIGTGQTSAERPVWVQWIPVHKNPAEPPGPDNPIIGRYAYWVDVENTKLHVDQP
                     LRDLRELPHYARLIGEPDRVEGGGSWHEQGDGSSKRQLRLALESSLPALEKNSSGPRN
                     RGGQDGIGFAPG"
     gene            complement(1106..1912)
                     /locus_tag="PLP58_21660"
     CDS             complement(1106..1912)
                     /locus_tag="PLP58_21660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ11203.1"
                     /translation="MKRESRNSKFGVRSSKFEVCRALRAVPGPPRAETPGANDAEHPR
                     GLAALPIRISSFEFRASPSHAAFTLPELLVSVTLTILVCVLVAEMSTSMLTSVSTASG
                     RMTSTQKLDRIRHLLGDDLARLPRLDDGEKRLLAAGDEEGWRVTLHLPARGDALRREG
                     RAWQVCRYHWIKSKALLMCEWLEDGEVSQSEAVLTGVIALHPEWLTQGTLQEQAEPAW
                     DSTALPGLLRLRVTLTDVWEEGRAEDQTAQPHRVREFRMILPVAGGGGGA"
     gene            complement(1909..2349)
                     /locus_tag="PLP58_21665"
     CDS             complement(1909..2349)
                     /locus_tag="PLP58_21665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ11204.1"
                     /translation="MKNLESRKTARGFSITEILMALALVAGSALPVLGLLSVGILDAK
                     EAGDHRLTANLRNTVQQLLSDPAWPEEAKPEGEWTAERGFDHEGRLLEPEEKNQAVLL
                     LRLRSLPAIGFESDHLETVQVSFYAPDRPDMLSRSVVQRVKTAP"
     gene            complement(2346..>3681)
                     /locus_tag="PLP58_21670"
     CDS             complement(2346..>3681)
                     /locus_tag="PLP58_21670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HRJ11205.1"
                     /translation="ARRPVIALTEAAEKAGLRAFRAAPDALASLDLSQERWLILPHAG
                     EADAVRLNALVERVAEMKKGSLKRLEFALEPGLAEKAAGLVKALAVAGAKAIRLPDPD
                     APWAQALVTHLVAQSPTLRARQAKPAPRPASAPAVTAAPAFTVLEVLISVVVLTFIVS
                     ITASGFQSLKRQDGVHEAAMDFAQVLRDARQMARRQNTLIRVALVSPALKDKLAEYGL
                     EATDLKAGCGVYAFRVPAHQLEPVAFTRPTAAHESAAHLRETAPLIRVPLPRSLIGGW
                     TLAAGQRSWQRWREHVWLGGPVLDRYLESDFEAFAKDLLHQPDSVWSEERDPDETRAS
                     PFSIYPPQMDRTPYRALPRPVTAALPESERVRLDDGTQIPAREIWGDSPVLRWPQNDA
                     LALPLPALDFTPEGRLACVAQEQLEFHFSPGPGRQPVYRVIIRSRDGEVHIE"
BASE COUNT          611 a         1107 c         1355 g          608 t
ORIGIN      
        1 ccgggggcga agccgatgcc gtcctggccg ccacggttgc gcggcccgct ggagtttttt
       61 tccaaagcgg gcaggctgga ctcgagggcg agacggagct ggcgcttgga actgccatcg
      121 ccctgctcat gccaggagcc gccgccttcg acgcggtcag gctcgccgat aaggcgtgcg
      181 tagtggggca gctcgcgcag gtcgcgcaaa ggctggtcca catgcagctt ggtgttttcc
      241 acatccaccc agtaggcgta gcggccgatg atgggattgt ccgggccggg cggttcggcg
      301 gggtttttgt gaaccggaat ccactgcacc cagacggggc gctcggcgct ggtctggccg
      361 gtgccgatgc ggaagctgtc gtcactccag cgggtgcctg tgcgctcgcg cactagggcc
      421 tcggcgtcct cgggcacgcc ggaaagcagg caggcgccgg gagcgaaggg attgcgcggg
      481 gtgttgagat tgaaggagag ggcggggttg taatccggat tcaggcggga aggcgtgccg
      541 ccctggacgg tgaggtgctt caggcgcggg gcgaaggctt tccaggagaa aagcgggatg
      601 tggcgcgggt tggcgggctc accctgatag gtggcgctga agggctggga aaagggcgcg
      661 ggattctcct ttgtgccagt gaaggcggcg gcgccagtat gtccgccgcg gttccacggc
      721 acgtcatagc ggcggacttc cagcaggccg ggcgtggcgg cgacatggcc ggtgaaagcg
      781 ccggttttct cgacgacgaa ggcctcgcgc agctttgcca gcacttcctg ccgggcggag
      841 agcatcagct cgtcggtgag cgtctcggcc tgatccaggc gggcgttggt gttttgaatg
      901 ctgctgctgc cgagaaaggc gatggcgacc acggccatga aaccggtgag caggagcacc
      961 cagacgaggg cggcgccgga ggggcggggg gagaagcgcg aacttcgaat ttcgaaattc
     1021 gaagttggaa ctgccatgat ggaaaccggc cgggcaaccg agcgcgcgcc aggcatcgaa
     1081 tttcgagttt cgaatttcga atttttcacg ctccacctcc tccccctgcc acgggcagga
     1141 tcatgcggaa ctcgcgcacg cggtgcggct gcgcggtctg gtcctcggcg cggccttcct
     1201 cccagacatc ggtaagggtg acgcgcaggc gcagcaggcc ggggagggcg gtgctgtccc
     1261 aggcgggttc ggcctgctcc tggagcgtcc cctgcgtgag ccattcggga tgcaaagcga
     1321 tgacgccggt gagcacggcc tcggactggc tgacctcgcc atcctccagc cactcgcaca
     1381 tgagcagcgc cttgcttttg atccaatggt agcggcagac ctgccaggcg cggccctcgc
     1441 ggcgcaacgc atcgccgcgc gcgggcaggt gcagggtgac gcgccagcct tcctcgtcgc
     1501 ctgcggccag caggcgcttt tctccgtcat caaggcgagg caggcgggcg aggtcgtcgc
     1561 cgagcaggtg gcggatgcgg tcgagctttt gcgtggaggt catgcgcccg ctggcggtgc
     1621 tcaccgaggt gagcatggag gtggacatct cggcgacgag cacgcagacg aggatggtga
     1681 gggtgacgga gacgaggagc tcggggaggg tgaaggcggc atgggacggg gaagctcgaa
     1741 attcgaagct cgaaattcga atggggaggg cggcaagtcc gcgcggatgt tcagcgtcat
     1801 tcgcacccgg tgtctccgcg cgcggcgggc cggggacagc ccgcagtgcc cggcaaactt
     1861 cgaacttcga acttcgaact ccgaacttcg aatttcgact ctcccgtttc atggtgccgt
     1921 cttgacgcgc tgcaccaccg agcggctgag catatccggc ctgtcagggg cgtaaaagct
     1981 gacctggacg gtctccaggt gatcgctttc aaaaccgatg gcgggcaggg agcgcaggcg
     2041 cagaagcagc actgcctggt ttttctcctc gggttccagc agccggccct catgatcaaa
     2101 gccacgctcg gcggtccact caccctcagg ctttgcctcc tcgggccagg cgggatcgga
     2161 aagcaactgc tgcacggtgt tgcggagatt ggcggtgagg cggtggtcac ccgcctcttt
     2221 ggcgtccagg atgcccacac tcaacagtcc cagcaccggc agcgccgaac ccgccacaag
     2281 ggcgagcgcc atgaggatct cagtgatact gaagccgcgt gcggtttttc tggattcgag
     2341 gtttttcatt cgatatgcac ctccccgtca cggctgcgga tgatgacccg gtacaccggc
     2401 tggcggccgg gaccggggct gaaatgaaac tccaactgct cctgcgcgac gcaggccaga
     2461 cggccctcgg gtgtgaaatc cagcgctggc agcggcagcg caagagcgtc attttgcggc
     2521 catcgcagca caggggaatc gccccagatt tcacgggcgg ggatttgcgt gccatcatca
     2581 aggcggacgc gctcgctttc cggcagtgcg gcggtgactg ggcgcggcag cgcgcggtaa
     2641 ggagtgcggt ccatctgcgg cggatagatg gagaaagggc tggcgcgggt ctcatcggga
     2701 tcgcgctcct cgctccagac gctgtccggc tggtgcagca ggtctttggc aaaagcttcg
     2761 aagtcagact ccaaatagcg gtccagcacc gggccgccga gccagacgtg ctcccgccat
     2821 ctctgccagg aacgctggcc tgcggcgagg gtccagccgc cgatgagact gcgcggcagg
     2881 ggcacgcgga tgagcggcgc ggtctcgcgc aaatgggcgg cgctctcgtg ggcggcggtc
     2941 gggcgggtga aggccaccgg ctcgagctgg tgcgcgggca cgcggaaggc atagacgccg
     3001 cagccggctt tgagatcggt cgcctccaga ccgtattcgg ccagcttgtc cttcaaagcg
     3061 gggctgacga gggccacgcg gatgagggtg ttttggcggc gggccatctg gcgcgcgtca
     3121 cgcaggacct gggcgaagtc catggccgcc tcatgcacgc catcctggcg tttgagggat
     3181 tgaaaaccgc tggcggtgat gctgacgatg aatgtcagca cgacgacgga aatgagcacc
     3241 tccaacacgg tgaaggcggg cgcggcagtg acagccgggg cggaggcagg gcgaggcgcg
     3301 ggctttgcct gacgggcgcg cagggtgggg ctttgcgcga cgagatgcgt gacgagagcc
     3361 tgcgcccaag gcgcgtccgg gtccggcagg cggatggcct tggcaccggc aacggcgagc
     3421 gccttgacga gacctgcggc cttttccgcg aggccgggct ccagggcgaa ctccaggcgc
     3481 ttgagcgagc cttttttcat ctccgccaca cgctcgacga gcgcgttcag gcggactgcg
     3541 tcggcttcgc ccgcgtgagg cagaatgagc cagcgctcct gcgagagatc caggctggcg
     3601 agcgcgtccg gcgcggcgcg gaaagcgcgc aggccggcct tttccgccgc ctcggtgagc
     3661 gcgatgaccg gcctgcgcgc t
//
LOCUS       DAOMYJ010000359         3678 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Prosthecobacter sp. isolate AMR_MDS_5629
            NODE_9008_length_3678_cov_17.684792, whole genome shotgun sequence.
ACCESSION   DAOMYJ010000359 DAOMYJ010000000
VERSION     DAOMYJ010000359.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867850
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prosthecobacter sp. (wastewater metagenome)
  ORGANISM  Prosthecobacter sp.
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Verrucomicrobiaceae; Prosthecobacter.
REFERENCE   1  (bases 1 to 3678)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3678)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.30
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:34:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,742
            CDSs (total)                      :: 4,684
            Genes (coding)                    :: 4,676
            CDSs (with protein)               :: 4,676
            Genes (RNA)                       :: 58
            tRNAs                             :: 55
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3678
                     /organism="Prosthecobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9008_length_3678_cov_17.684792"
                     /isolate="AMR_MDS_5629"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1965333"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1065)
                     /locus_tag="PLP58_21675"
     CDS             complement(<1..1065)
                     /locus_tag="PLP58_21675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ11206.1"
                     /translation="MEKLILTLLAVFTASASAETLLLRGATVHTVAGGTLAPGDVLVR
                     GGKIAEVAAAITAKADRVLDLKGLHLYPGLVSPGTDLGLVEISGVRASVDDRETGDFI
                     PEVESWLAVNPDSELIPVARANGITHFAPIPQGRHIAGTSALLAARGWTIEDMLVRRH
                     TAMHLFWPDHSLSIPGPNPSPNSKSLEDQARERKEKVREIERFFADAEAWAKRPPDSP
                     PVPAWQAMQPVLSGEVPLMIHAQDAREIRAALKWSEGRPGLRVILYGARDAWQLADEL
                     AQRKIPVIYSQVFALPARFSDAYDAHFSAPAILHKAGVTVAISEGLENASASGQRNLP
                     YIAAQAAAFGLPADAALAAIT"
     gene            complement(1053..>3678)
                     /locus_tag="PLP58_21680"
     CDS             complement(1053..>3678)
                     /locus_tag="PLP58_21680"
                     /inference="COORDINATES: protein motif:HMM:NF014081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HRJ11207.1"
                     /translation="PPDARVHDMSGTTIYAGFIDMHAALGKTSAARATASDEGPPVDD
                     LAAGAGSGFLGVASAAGEAGAKSVVTPERRMAREFAPDKKTLDALRHEGFTAANFVPD
                     SGVIRGVSAFVSLGSGDPDEAVIRPETFQHMAIDAPRSSSSESSRPRAYPGSLMGVIS
                     VIRQTLLDARFQARDHAHFSQNPASRPRPAFSTMLDALQPVTAQKMPVVFEPASVLMV
                     DRAARLAGEFELRPLILATGQEWRRPDIMRGIDAPFIVPVDFPEAVKLPDDEDWQAMP
                     LDQLRAWDHAPGNAALLRKEGREIALTTHGIARKTSFRPNLRLAIARGLSQDDALAAL
                     TTVPARLCGVADQLGSIAPGKLAHLTVFENGRAFDDDARVREVWIDGRQYPAPFRDEK
                     KPAPKRDAANPRLTLTAAPSNHDRGPLLEPKSVLIRNATLWTCGPEGRIENASLLVTD
                     GKIVRAGRFKTDPGPGTHVIDLPGIHVTPGLIDCHSHSMIMGGVNEGTLPSTAMVRVA
                     DVLNSESETIHQQLAGGLTVANLLHGSANPIGGQNCVIKLRDGAPPEGLKFVDAPAGI
                     KFALGENVKQSNWGDAFKSRFPQSRMGVPAFHTNRFTAARHYMAAVEKAASGGPPVRR
                     DLELEAIAEILCGERLIHCHSYRQDEILAFLRVMEGFKVRVATLQHILEGYKVANEIA
                     RHGAGASAFSDWWAYKFEVLDAIPHAGAIMHERGILVSFNSDSSDHARRMNLEAAKAV
                     KYGGVSEEEALKFVTLNPAKQLRIDARAGSLEPGKDGDFAIWNGHPLDTRSVCLQTWI
                     EGRQYFEREAARQRASARHSERLALIEKARKSTAGPGSASEEDAKAGAKFFLRALEER
                     QGHLCVDCCMDRELSWKN"
BASE COUNT          552 a         1076 c         1377 g          673 t
ORIGIN      
        1 ggtgatggcg gcgagcgcgg cgtcggcggg caggccgaag gcggcggcct gcgcggcgat
       61 gtagggcaga ttgcgctggc cggaggcgct ggcgttctcg aggccttcgc tgatcgccac
      121 ggtgacgccg gctttgtgca ggatggcggg cgcgctgaag tgcgcgtcat aggcgtcact
      181 gaagcgcgcg ggcagcgcga agacctggct gtagatgacg ggaatcttcc tttgcgcgag
      241 ttcatcggcg agctgccatg cgtcgcgcgc gccgtagaga atgacgcgca gtccaggccg
      301 tccttcggac catttcaagg cggcgcggat ttcacgcgcg tcctgcgcat gaatcatgag
      361 tggaacctcg ccgctcagca cgggctgcat ggcctgccag gcgggcacgg ggggactgtc
      421 tggagggcgt ttggcccagg cttcggcgtc ggcgaagaag cgctcgattt cccggacttt
      481 ttctttccgc tcgcgcgcct ggtcttcgag ggacttcgag ttgggggagg gattcgggcc
      541 cggaatgctg agcgaatggt cgggccagaa gagatgcatg gcggtgtgac gcctcacgag
      601 catgtcctcg atcgtccagc cgcgcgcggc aagcagggcg gaggtgccgg cgatgtgcct
      661 gccctgcggg atgggggcga agtgggtgat gccgttggcg cgcgcgacgg ggatgagttc
      721 ggagtcggga ttgaccgcca gccaggactc cacctccggg atgaagtcgc ccgtttcccg
      781 gtcgtccacg gaggcccgca cgccgctgat ttcgacgagg cccaggtcgg tgcccggact
      841 gacgaggccg gggtagaggt gcagcccttt gaggtcgagc acgcggtccg cctttgcggt
      901 gatggcggcg gcgacttcgg cgattttgcc gccgcgcacg agcacgtcac cgggtgcgag
      961 ggtgccgccg gccacggtgt ggacggtggc gccgcgcagc agcagggttt cagcggaggc
     1021 tgaggccgtg aagacggcga ggagggtgag gatcagtttt tccatgagag ttcgcggtcc
     1081 atgcagcagt ccacgcagag atggccctgg cgctcctcca gggcgcggag gaagaatttg
     1141 gccccggctt tggcgtcctc ttctgaagcg gagccggggc cggcggtgga tttcctcgct
     1201 ttttcaatga gggcgaggcg ctcgctgtgg cgcgcgctgg cgcgctggcg ggcggcttca
     1261 cgctcgaagt attggcggcc ttcgatccag gtctgcaaac agacgctgcg ggtgtcgagc
     1321 gggtgcccgt tccagatggc gaagtcgccg tccttgcccg gctccagtga gccggccctg
     1381 gcgtcaatgc ggagctgttt ggcggggttg agggtgacga atttcagggc ctcctcctcg
     1441 ctgacgccgc cgtatttcac ggccttggcg gcttccaggt tcatgcgcct ggcgtggtcc
     1501 gaggagtcgg agttgaaaga gacaaggatg ccgcgctcgt gcatgatggc gcccgcgtgc
     1561 gggatggcat cgagcacttc gaatttgtag gcccaccagt cggagaaggc ggaggcgccc
     1621 gcgccgtgcc gggcgatttc attggcgact ttgtagccct ccaggatgtg ctggagcgtg
     1681 gccacgcgaa ccttgaagcc ctccatgacg cgcaggaagg cgaggatttc atcctgccgg
     1741 tagctgtggc agtggatgag acgctcgccg caaaggatct cggcgatggc ttccaactca
     1801 aggtcgcggc gcactggcgg gccgccgctg gccgccttct cgacggcggc catgtagtgc
     1861 cgggcggcgg tgaagcggtt ggtgtggaag gcggggacgc ccatgcggct ctgcgggaag
     1921 cgggacttga aggcatcccc ccagttggat tgcttcacat tttcgccgag ggcgaacttg
     1981 atgccggcgg gggcgtccac gaatttcagg ccttcgggcg gcgcgccgtc gcgcagtttg
     2041 atgacgcagt tctggccgcc gatggggttg gcggaaccat gcagaagatt ggccacggtg
     2101 agaccgcctg ccaactgctg gtgaattgtt tcggactcgg agttgagcac gtcggcgacg
     2161 cgcaccatgg ccgtgctggg cagggtgccc tcgttgacgc cgcccatgat catgctgtgg
     2221 ctgtggcagt ctatgaggcc gggcgtcaca tggatgccgg gcaggtcaat cacatgggtg
     2281 cccgggccgg ggtcggtctt gaagcggccg gctctgacaa ttttgccgtc agtgaccagc
     2341 aggctggcgt tttcgatcct tccctccggg ccgcaggtcc acagggtggc attgcgaatg
     2401 aggacacttt tcggttcgag caaaggtcct cggtcatggt tggagggagc ggcggtgagt
     2461 gtgagacgcg ggttggcagc gtcccttttt ggagccggtt tcttctcatc gcggaaggga
     2521 gcggggtatt gcctgccatc aatccacacc tcgcgcacac gcgcgtcatc gtcgaaggcg
     2581 cggccgtttt cgaacacggt caaatgcgcg agttttcccg gcgcgatgct gccgagctgg
     2641 tctgcgactc cgcagagtct ggcgggcacc gtggtcaacg cggcgagcgc gtcgtcctgg
     2701 ctcaggccgc gcgcgatggc gaggcgcagg ttggggcgga aggaggtctt cctggcgatg
     2761 ccgtgcgtgg tcagcgcgat ttcccggccc tctttgcgca gcagggcggc attacccggc
     2821 gcgtggtccc aggctctcag ttgatcgagc ggcatggcct gccagtcctc gtcatcgggc
     2881 agcttcacgg cttcgggaaa atccaccggc acgatgaagg gcgcgtcaat gccgcgcatg
     2941 atgtccggcc tgcgccattc ctggccggtg gcgaggatga gcggacgcag ctcaaactcg
     3001 ccagcgagcc gagccgcacg gtccaccatg agcacgctgg cgggttcaaa gaccaccggc
     3061 attttttggg cggtcacagg ctgcagggcg tccagcatgg ttgaaaatgc ggggcgcggc
     3121 ctggaggcgg ggttttggga gaaatgtgcg tggtccctgg cctggaagcg cgcgtccagg
     3181 agcgtctgcc ggatgacgga gatgacgccc atgagcgagc ctggataggc cctggggcgc
     3241 gaggactccg aactggagga gcgcggggcg tcaatcgcca tgtgttgaaa agtctccgga
     3301 cggatgacgg cctcgtccgg gtcgccggag ccgagagaga caaaggcgct gacaccgcgg
     3361 atgacgccgc tgtccggcac gaagttcgcc gccgtgaaac cttcgtgacg cagggcgtcg
     3421 agggtttttt tgtccggcgc gaactcgcgc gccatgcgcc gctccggtgt gacgacggat
     3481 tttgcgcccg cctctcccgc cgcagaggcg actccgagaa agccgctgcc ggcgccggcg
     3541 gcgaggtcat ccacgggcgg tccctcgtcg cttgcggtgg cgcgcgcggc gctggttttc
     3601 ccgagggcgg cgtgcatgtc aatgaagcct gcgtagatcg tggtgccgct catgtcatgc
     3661 acgcgcgcgt ccggcggc
//
LOCUS       DAOMYJ010000360         3670 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Prosthecobacter sp. isolate AMR_MDS_5629
            NODE_9041_length_3670_cov_16.134716, whole genome shotgun sequence.
ACCESSION   DAOMYJ010000360 DAOMYJ010000000
VERSION     DAOMYJ010000360.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867850
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prosthecobacter sp. (wastewater metagenome)
  ORGANISM  Prosthecobacter sp.
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Verrucomicrobiaceae; Prosthecobacter.
REFERENCE   1  (bases 1 to 3670)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3670)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.30
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:34:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,742
            CDSs (total)                      :: 4,684
            Genes (coding)                    :: 4,676
            CDSs (with protein)               :: 4,676
            Genes (RNA)                       :: 58
            tRNAs                             :: 55
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3670
                     /organism="Prosthecobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9041_length_3670_cov_16.134716"
                     /isolate="AMR_MDS_5629"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1965333"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1315
                     /locus_tag="PLP58_21685"
     CDS             <1..1315
                     /locus_tag="PLP58_21685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ11208.1"
                     /translation="DPAPALRVARGLAGAEGVVVLVTDHRLDETPAHGPQVLAVGEAT
                     DNVGWAGVRVEEKDGQWLWHALVKNHGRSPQQREWRALAAGVESPAKTLALAPGEMLS
                     LSGPFPAEGDGRLLLRLSGDALPLDDELPLVRPAPKTLAVFAPAEGLPETMRHLLESL
                     PHTGRSASAAAADLEVAVWREATLLPEGRHALVILPPPAKDAPFPKGGIVAEPHPLME
                     GLNWQGLLPREAPAPPRLTRDSVLLWQGERPLILLRQMPAGGRQLLCLFDPDSSGALR
                     MPAFAVLAHRFLESLRAEKIAPESANYDTRQRLHLAHLGGPDAPPLVIESQDESREVP
                     PAQGGLLRAPARPGHFTVSQGGTVLLTAAAHFADTREADLSHAAAWGDPAMIRADLTQ
                     TRRETSPHQPLWLLALLLLLMGSWWWSRERKILPLQSTEASTNAS"
     gene            1395..>3670
                     /locus_tag="PLP58_21690"
     CDS             1395..>3670
                     /locus_tag="PLP58_21690"
                     /inference="COORDINATES: protein motif:HMM:NF012321.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HRJ11209.1"
                     /translation="MTLHHPEWLALFPLLLLAGWMLPRLGVLRPLRLALLALVTLILA
                     QPQWRRLADGVDLWVLLDQSVSAEDALARRLPEWERLLEKSRGAQDRVYYLDYAAEVM
                     KRGTQGAEMMERARLQTLTAQAVSQALALAQREGRGRPARVLAVTDGYATEPLAGLAE
                     KLARQGVELDYLLARASDERDYRVAGLKMPARAQLGEPFLIEVSVRGSADADVPLTLL
                     RNGRELKTDTVPVRLGSGVVRFTDRLIQAGAAHYEAVILPGQDAHTGNNRHEAMIEII
                     GGPRVLLLTAYADDPVAGVLIKQSFEVETVTATRSVLPGQLAGAKCVILNNVPAYELP
                     GDFLTALDFHVREQGGGLLMAGGRHSFAAGGYFESALDPLLPVSMEMKQEHRKLMVAM
                     AIVMDRSGSMSMTVRNGFTKMSLANEGAGNAIRFLGPQDLLAVFAVDSEAHEVVPLQQ
                     IGPNREKMEKSVRRIESMGGGIFVYNGLKAAWDQLKKAPAGQRHIILFSDAADSEQPD
                     AYRALLEEITRNSGTVSVIALGTRSDPDAAFLDDIAARGKGRIFYTDRAEELPGIFSQ
                     ETVAVARGAFIAEPVGAQSAPGWLEISGQGMEWPAQVDGYNLSYLRDWAAQALVSKDE
                     YAAPLVAFGARGTGRTAAVSFPLGGEHSQSARAWPKYGDFLQTLTRWLMGETLPPGIG
                     LRHRLEGNTLTLDLLYDEEWEDTLRAKAPRIMLAHGQGAEARQEIAWERVAPGHFQAS
                     TVLAEGAMTRGAVRVGAQA"
BASE COUNT          551 a         1314 c         1245 g          560 t
ORIGIN      
        1 tgatccggcc cccgcgctgc gcgtggcgcg cgggctagcc ggcgcggagg gcgtggtggt
       61 gctcgtcacg gatcaccgac tggacgagac tcccgcgcat ggcccgcaag tgctggccgt
      121 gggcgaggcg acggacaacg tcggctgggc cggcgtgcgt gtggaggaaa aggacgggca
      181 atggctctgg cacgcgctgg tgaaaaatca cggccgcagc ccgcagcagc gcgaatggcg
      241 cgcgcttgcg gcgggagtgg agtctcccgc gaaaacgctc gcgctggccc ccggcgagat
      301 gctttccctc tccggccctt ttcccgccga gggcgacggg cgcctgctcc tgcgtctctc
      361 gggcgacgcg ctgcctctgg atgacgagct gcccctggtg cgcccggctc cgaaaacgct
      421 cgccgttttc gcgcctgcgg agggcctgcc ggagaccatg cggcatttgc tggaaagcct
      481 gccgcacaca ggccgcagtg ccagcgccgc agcggcggat ctggaggtgg cggtctggcg
      541 cgaggccacg cttttgcccg aaggccggca cgcgctcgtc atcctgccgc ctccggcaaa
      601 ggacgcgccc tttcccaaag gcggcatcgt ggccgagccg catccgctca tggagggact
      661 gaactggcag ggcctgctgc cgcgcgaagc gccggcgccg ccgcgcctga cgcgggacag
      721 cgtgctgctc tggcagggcg agcgcccgct catcctgctg cgccagatgc ccgcaggcgg
      781 acggcagctt ctttgtctct tcgatcccga cagctccggc gctttgcgaa tgccggcctt
      841 cgccgtgctg gcgcatcgtt ttttggaaag cctgcgcgcc gaaaaaatcg cgcccgagtc
      901 cgccaactac gacacccgcc agcgccttca cctggcccac cttggcggcc cggacgcgcc
      961 gccactcgtc atcgaaagcc aggacgagtc gcgcgaggtt ccgcccgcgc aaggcggcct
     1021 gctgcgcgcc cccgcgcggc ccggccactt caccgtgagc cagggcggca ccgtgctgct
     1081 caccgcagcc gcgcatttcg ccgacacgcg cgaggcggac ctctcccatg ccgccgcctg
     1141 gggtgacccc gccatgatcc gcgcggactt gacccaaacc cgccgcgaga ccagccccca
     1201 ccagcctctc tggctcctgg ccctgctgct tctgctcatg ggcagttggt ggtggagcag
     1261 agagcggaaa atcctgccac tgcaaagcac ggaagcctcg acaaatgcat catgagttcc
     1321 acccccgtcg ccttcatcct tttgcgtttt tttgagtttt ttgcggccat tcctcatcgc
     1381 ccacgctctc tgccatgacc ctccaccacc ccgaatggct cgcgcttttc cccctgctgc
     1441 tgctggcggg ctggatgctg ccgcgcctgg gggtgctgcg ccccttgcga ctggccttgc
     1501 tggcgctggt cacgctcatc ctggcgcagc cgcagtggcg caggctggcg gatggcgtgg
     1561 atctctgggt gctgctggac cagtccgtct ccgccgagga tgccctggcg cgccgcctgc
     1621 cggagtggga gcggctgctg gaaaagtcac gcggcgcgca ggaccgcgtc tattatctcg
     1681 actacgctgc ggaggtgatg aagcgcggca cgcagggcgc ggagatgatg gagcgcgccc
     1741 ggctccagac cctcacggcg caggccgtct cccaggcgct ggccctggcg caacgcgagg
     1801 ggcgcggacg cccggcgcgc gtgctggctg tcacagatgg ttatgccacc gagccgctcg
     1861 ccggcctggc ggaaaagctc gcgcgccagg gagtggagct ggattacctg ctggcgcgcg
     1921 ccagtgacga gcgggactac cgcgtggcag ggctgaaaat gcccgcccgc gcgcagcttg
     1981 gcgagccttt cctcatcgag gtcagcgtgc gcggcagcgc ggatgcggat gtgccgctca
     2041 ccctgctgcg caatggccgc gagctgaaga ccgacaccgt gcccgtgcgc ctcggcagcg
     2101 gcgtggtccg cttcacggac cgcctcatcc aggcaggcgc ggcgcattac gaggcggtga
     2161 ttcttcccgg ccaggacgcg cacacgggca acaaccgcca cgaggccatg atcgagatca
     2221 tcggcggccc gcgcgtgctg ctgctcaccg cctatgccga cgacccggtg gccggggttt
     2281 tgatcaagca gagcttcgag gtcgagaccg tcaccgccac ccgatccgtg ctgcccggcc
     2341 agctcgcggg ggccaaatgc gtgatcctta acaacgtgcc cgcctacgag ctgccggggg
     2401 actttctcac cgcgctcgac tttcacgtgc gcgagcaggg cggcggcctg ctcatggccg
     2461 gcgggcggca cagctttgcc gcgggcggct acttcgagtc ggcgctggac cctcttttgc
     2521 ccgtgtccat ggagatgaag caggagcacc gcaagctcat ggtggccatg gccatcgtca
     2581 tggaccgcag cggctccatg agcatgaccg tgcgcaacgg cttcaccaaa atgtccctcg
     2641 ccaacgaggg cgcgggcaac gccatccgct tcctcggccc gcaggatttg ctggccgtct
     2701 tcgccgtgga cagcgaggcg cacgaggtgg tgccgctcca gcagatcggc ccgaaccgcg
     2761 agaagatgga gaaatccgtg cgccgcatcg agagcatggg cggcggcatc tttgtctata
     2821 acggcctcaa ggccgcctgg gaccagctca aaaaagcgcc cgccgggcag cggcacatca
     2881 tccttttctc cgacgccgcc gactccgagc agccggacgc ctaccgcgcg ctgctggagg
     2941 agatcaccag gaacagcggc accgtcagcg tcatcgccct cggcacccgc tccgacccgg
     3001 acgcggcctt cctcgacgac atcgccgcgc gcggcaaagg ccgcattttt tacaccgacc
     3061 gtgccgagga gctgcccggc atcttcagcc aggagaccgt ggccgtggcg cgcggcgcct
     3121 tcatcgccga gccagtgggc gcgcagtccg cgcccggctg gctggagatc agcggccagg
     3181 gcatggaatg gcccgcgcag gtggacggct acaacctcag ctacctgcgg gactgggccg
     3241 cgcaggcgct cgtcagcaaa gacgagtatg cggcgccgct cgtggccttc ggcgcgcgcg
     3301 gcacgggacg cactgccgcc gtctcctttc ccctcggcgg ggagcattcc caaagcgcgc
     3361 gcgcctggcc gaagtatggc gactttttgc aaacgctcac gcgctggctc atgggcgaga
     3421 ccctgccgcc cggcatcggc ctgcgccacc ggctggaggg aaacacgctc acgctcgatc
     3481 tgctctacga tgaggaatgg gaggacaccc tgcgcgccaa ggccccgcgc atcatgctgg
     3541 cccacggcca gggcgcggaa gcgcggcagg agatcgcctg ggagcgcgtc gcccccggcc
     3601 acttccaggc ctcgaccgtg ctggcggaag gcgccatgac acgcggcgcg gtgcgcgtgg
     3661 gcgcgcaggc
//
LOCUS       DAOMYJ010000361         3664 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Prosthecobacter sp. isolate AMR_MDS_5629
            NODE_9065_length_3664_cov_19.398171, whole genome shotgun sequence.
ACCESSION   DAOMYJ010000361 DAOMYJ010000000
VERSION     DAOMYJ010000361.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867850
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prosthecobacter sp. (wastewater metagenome)
  ORGANISM  Prosthecobacter sp.
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Verrucomicrobiaceae; Prosthecobacter.
REFERENCE   1  (bases 1 to 3664)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3664)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.30
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:34:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,742
            CDSs (total)                      :: 4,684
            Genes (coding)                    :: 4,676
            CDSs (with protein)               :: 4,676
            Genes (RNA)                       :: 58
            tRNAs                             :: 55
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3664
                     /organism="Prosthecobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9065_length_3664_cov_19.398171"
                     /isolate="AMR_MDS_5629"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1965333"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1249)
                     /locus_tag="PLP58_21695"
     CDS             complement(<1..1249)
                     /locus_tag="PLP58_21695"
                     /inference="COORDINATES: protein motif:HMM:NF014628.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0019516 - lactate oxidation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lactate utilization protein B"
                     /protein_id="HRJ11210.1"
                     /translation="MHPAAAREFLSDPARAAWHDQTLWMVRVKRDRMAARVPEWEQLR
                     EAASRIKEHTLSRLAEYLEEFSARAEANGVRVHWAADAEEHNRIVHGILAARGVTRMV
                     KSKSMLTEECHLNPYLEARGIEVVDTDLGERIVQFKNEPPSHIVMPAIHLKRAEVGAV
                     FHEHLGTPAGMEDAQELAEAARQHLRGHFLGAQAALTGVNFAIAETGGFVVCTNEGNA
                     DMGAHLAPVHIACMGIEKLIPKAEHLGVFLRLLARSATGQPSTVFSSHFLKPRPGRET
                     HLVLVDNGRSRQLARADFRKSLACIRCAACFNTCPVYRRSGGHSYSWLISGPIGSILA
                     PNVDKKAHSDMPFASTLCGSCTAVCPVKIDIHAQLLKWRQEVVAAGGSGRAKPLLMRL
                     TAFVLRRPALYRLGGALMRLAGPL"
     gene            complement(1283..2005)
                     /locus_tag="PLP58_21700"
     CDS             complement(1283..2005)
                     /locus_tag="PLP58_21700"
                     /inference="COORDINATES: protein motif:HMM:NF014774.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HRJ11211.1"
                     /translation="MRLGLFIPCYMDQLYPRAAHATLRLLERLGHEVECPDTPACCGQ
                     PMSNSGHAALGEDFVDAFARRYAGYERVVSPSASCLLHLKEGLEARGHPLAHHAHELC
                     EFLHDVERVEAFDAAFPHRVGLHQGCHGLRGLGLGRPSERMEPHFSKPERLLKRVRGL
                     ELVELDRPDECCGFGGSFAVFESDVSAAMGSDRVADHAGHGAEFITSTDMSCLMHLGG
                     LAARSHPRIKVLHIAEILAGEV"
     gene            complement(2026..2802)
                     /locus_tag="PLP58_21705"
     CDS             complement(2026..2802)
                     /locus_tag="PLP58_21705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009959611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HRJ11212.1"
                     /translation="MSSIFELFSLQGRTAVVIGGTGELCGAIAEGYASAGAEVVLVGR
                     DPAKAEKRLETIHAAGGTGYFVGADASRKADLQLLLDQVIERSGGCQILVNGAGVNSA
                     TPFLDISEEEYDRIMGINTKAVFLACQVFGKYFTEQKVEASIINLGSMSGLLPLSRVF
                     TYSMSKGAVHNLSKNLAREWAPQGIRVNTLVPGFFPAEQNKKVLTPDRVAKIMGHTPM
                     NRFGSASELAGAALLLAAGAGSFVTGHELVVDGGYSSMTI"
     gene            2954..>3664
                     /locus_tag="PLP58_21710"
     CDS             2954..>3664
                     /locus_tag="PLP58_21710"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015679.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_function: GO:0071972 - peptidoglycan L,D-transpeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HRJ11213.1"
                     /translation="MTLSALPLPKPARRFAAASLPMLAAGLLLSGCVSKPKSEVVVSV
                     KDQKMGIYDQGKLKKQFVISTSKFGVGDQPNSYRTPLGKHEVIAKIGHGLPTGAVLKS
                     RHWNGEVLKPNAPGRDPIVSRILWLRGLEKDNRNAMRRYIYIHGTTEESRLGTPASYG
                     CIRMGMKDVVEAFNEVGIGAKVVITKDHLPKGAPAPKEPGPDAEPAKTEAQAGEKAAA
                     PESPASTAPAAGDAPALAA"
BASE COUNT          620 a         1383 c         1102 g          559 t
ORIGIN      
        1 ccagcggccc ggccagccgc atcagcgcgc cgcccaggcg gtagagcgcg ggccggcgca
       61 gcacgaaggc ggtgaggcgc atgagcagcg gcttcgcgcg cccggagccc cccgctgcca
      121 ccacctcctg ccgccatttc aggagctgcg cgtggatgtc tatcttcacc gggcacacgg
      181 cggtgcagga gccgcacaga gtggaggcga agggcatgtc actgtgcgct tttttgtcca
      241 cgttcggagc caggatgctg ccgatcggcc ccgagatgag ccagctgtaa ctgtgcccgc
      301 cgctgcgccg gtagaccggg caggtgttga agcaggccgc gcagcggatg caggccaggc
      361 tcttgcggaa atccgcgcgc gcgagctggc ggctgcggcc gttgtccacc agcacgaggt
      421 gcgtctcccg gcccggacgc ggcttgagga agtgggagct gaacaccgtg ctcggctgcc
      481 ccgtggcgct gcgcgccagc agccgcagga agacgcccag gtgctccgcc ttcgggatca
      541 gcttctcgat gcccatgcac gcgatgtgga ccggcgccag gtgcgctccc atgtcggcat
      601 tcccctcgtt cgtgcagacg acaaagccgc ccgtctccgc gatggcgaag ttcacccccg
      661 tgagcgccgc ctgcgcgccg aggaaatgcc cgcgcagatg ctggcgcgcg gcctccgcca
      721 gctcctgcgc gtcctccatg cccgccggcg tgcccagatg ctcgtggaag accgcgccca
      781 cctccgcccg cttcagatga atggccggca tcacgatgtg gctcggcggc tcgtttttga
      841 actgcacgat gcgctcgccc aggtccgtgt ccaccacctc gatgccgcgc gcctccagat
      901 agggattcag atggcactcc tcggtgagca tggacttgct cttcaccatg cgcgtgacgc
      961 cgcgcgcggc caggatgccg tggacgatgc ggttgtgctc ctccgcgtcc gccgcccagt
     1021 gcacccgcac cccgttggcc tccgcccgcg cgctgaactc ctccaggtac tccgccagcc
     1081 gtgaaagcgt gtgctccttg atccgcgagg ccgcctcgcg caactgctcc cactccggca
     1141 cccgcgccgc catccggtcg cgcttcaccc gcaccatcca cagcgtctgg tcatgccacg
     1201 ccgcgcgcgc gggatcgctc agaaactctc gtgcggcggc ggggtgcatg gcggcgggga
     1261 tcttgttttg caaatgccgc gctcacactt ccccggccag gatttcggcg atgtgcagca
     1321 ccttgatccg gggatgcgag cgcgccgcca gcccgcccag atgcatcagg caggacatgt
     1381 ccgtgctggt gatgaactcc gccccatgcc ccgcgtggtc cgccacgcgg tcgctcccca
     1441 tcgccgccga gacatcgctc tcaaaaaccg cgaaactgcc gccgaacccg cagcactcgt
     1501 cgggccggtc cagctccacc agctccagcc cgcgcaccct tttcagcagc cgctccggct
     1561 tggaaaaatg cggctccatg cgctcgctcg gcctgcccag gcccagcccg cgcaggccgt
     1621 ggcagccctg gtgcaggccc acgcggtgcg gaaaggccgc gtcaaacgcc tccacccgct
     1681 ccacatcgtg cagaaactcg cacagctcgt gcgcgtgatg cgccagcgga tgaccgcgcg
     1741 cctccagccc ctccttcaaa tgcagcaggc acgaggccga gggcgagacc acgcgctcat
     1801 agcccgcgta gcgccgcgcg aaggcgtcca caaaatcctc ccccagcgcc gcatggccgg
     1861 agttcgacat cggctggccg cagcaggccg gcgtgtccgg gcactccacc tcgtgcccca
     1921 gccgctccag cagccgcaga gtggcatggg cggcccgcgg gtagagctgg tccatgtagc
     1981 aggggatgaa aaggccgagg cgcatgacgg gcgggccttt cctgctcaga tcgtcatcga
     2041 gctgtaaccg ccgtccacca ccagctcatg gccggtgacg aacgaccccg cgcccgccgc
     2101 cagcagcagc gccgcgccgg ccagttccga ggcgctgcca aagcggttca tcggcgtgtg
     2161 gcccatgatc ttcgccacgc ggtccggcgt cagcaccttc ttgttctgct cggctgggaa
     2221 aaagcccggc accagcgtgt tcacccggat gccctgcggc gcccactcgc gcgccaggtt
     2281 cttgctcagg ttgtgcaccg cgcccttcga catcgagtaa gtgaagacac ggctcagcgg
     2341 cagcaggccg gacatcgagc cgagattgat gatcgaggcc tccaccttct gctcggtgaa
     2401 gtatttgccg aaaacctggc acgccagaaa caccgccttc gtgttgatgc ccatgatccg
     2461 gtcatactcc tcctcgctga tgtcgaggaa aggcgtcgcc gagttcaccc ccgcgccgtt
     2521 gaccaggatc tggcagcccc cggagcgctc gatcacctgg tccagcaaaa gctgaaggtc
     2581 cgccttccgc gaggcgtccg cgcccacgaa gtagcccgtg ccgcccgccg cgtggatcgt
     2641 ctccagccgc ttctcggcct tggcggggtc ccgccccacc agcaccacct ccgcgcccgc
     2701 gctggcgtag ccttcggcga tcgcgccgca tagttcgcca gtgccgccga tcaccacggc
     2761 ggtccggccc tgcaaagaaa agagttcgaa gatcgaggac atgagagcgc atagaaagca
     2821 agagatgcgc ccctgtcgaa tgaagaacac gccatgcgcc gccgtccgca attctcaccg
     2881 attgaaatcc cccgccgcga atgttctttt ggagattgcg atttacttag cttttgttaa
     2941 ctttcccagt gcaatgactc tctccgcact tccgctcccc aagcccgcgc gccgtttcgc
     3001 cgccgcctcc ctgcccatgc tcgcggccgg cctcctgctc agcggctgcg tctccaagcc
     3061 caagtccgag gtcgtcgtca gcgtcaagga tcaaaagatg ggcatctatg accagggcaa
     3121 gctcaagaag cagttcgtca tctccacctc caagttcggc gtcggcgacc agcccaacag
     3181 ctaccgcacc cccctcggca agcatgaggt catcgccaaa atcggccacg gcctgcccac
     3241 cggcgccgtg ctcaagagcc ggcattggaa tggcgaggtg ctcaagccca acgcccccgg
     3301 ccgcgacccc atcgtctccc gcatcctctg gctgcgcggc ctggagaaag acaaccgcaa
     3361 cgccatgcgc cgctacatct acatccacgg caccaccgag gagagccggc tcggcacccc
     3421 cgccagctac ggctgcatcc gcatgggcat gaaggatgtc gtcgaagcct tcaacgaggt
     3481 cggcatcggc gccaaagtcg tcatcaccaa agaccacctc cccaaaggcg cccccgcgcc
     3541 caaggagccc ggccccgatg ccgaaccggc gaaaaccgaa gcccaagccg gcgaaaaagc
     3601 cgccgccccc gaatcccccg cctccacagc gcccgcggcc ggtgacgcgc ccgccctggc
     3661 cgcc
//
LOCUS       DAOMYJ010000362         3645 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Prosthecobacter sp. isolate AMR_MDS_5629
            NODE_9146_length_3645_cov_23.232033, whole genome shotgun sequence.
ACCESSION   DAOMYJ010000362 DAOMYJ010000000
VERSION     DAOMYJ010000362.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867850
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prosthecobacter sp. (wastewater metagenome)
  ORGANISM  Prosthecobacter sp.
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Verrucomicrobiaceae; Prosthecobacter.
REFERENCE   1  (bases 1 to 3645)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3645)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.30
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:34:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,742
            CDSs (total)                      :: 4,684
            Genes (coding)                    :: 4,676
            CDSs (with protein)               :: 4,676
            Genes (RNA)                       :: 58
            tRNAs                             :: 55
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3645
                     /organism="Prosthecobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9146_length_3645_cov_23.232033"
                     /isolate="AMR_MDS_5629"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1965333"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1448)
                     /locus_tag="PLP58_21715"
     CDS             complement(<1..1448)
                     /locus_tag="PLP58_21715"
                     /inference="COORDINATES: protein motif:HMM:NF040438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase domain-containing protein"
                     /protein_id="HRJ11214.1"
                     /translation="MTALPRLLLATFLCSGARAEIARIEITERAPFADGRVFGNAGAY
                     ERLKGRMFIETDPANQANERISDLQRAPRNARDKVESWTDFFLLKPVDATKGNGVLLY
                     DVNNRGNMLALWTFNDGERTNDPKTEAHAGHGFLMKHGFSVLWCGWNGEVQADDTQRL
                     LCGLPIATENGKTITGKAHLEITSTEKVFSRAFSWSPWGIGAAFPSVSLDNTDATLTM
                     RPHRVAGGVEVPRDEWAFGRWENEKLIPDATHVYVKAGLRPGWLYDLAYTAKEPRVTG
                     LGLTAMRDCVAFFRHGDAKTNPLAGAVQKACVFGISQSGRVIHHFLYEGLNGDEQGRI
                     VFDGALIHVAGSGKGMFNHRFRMSTEYGTQHEGHLSGSEFFPLAPLPQTDPVTGESGD
                     SLARSRKSGHTPRILFTQSSTEYWSRAASLLHTDVEGQTDLTLPDDVRVYLVAGAQHL
                     GKSDGTPGICQQPRNTLDDRGPVLRAMLMHLV"
     gene            1567..2211
                     /locus_tag="PLP58_21720"
     CDS             1567..2211
                     /locus_tag="PLP58_21720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009963497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HRJ11215.1"
                     /translation="MKTLLCFGDSNTWGFRPDSINAPHPERHALDVRWTGVLARELGP
                     EWRVIEEGQNGRTTVHDDPLNVARNGKTYLPASLESHMPVDLVIMMLGTNDLKALHNL
                     PPGEIAAGAAVLARMILGSAAGPGHRAPRLLLVSPPAIGDLTHLPDLAAKIPNGIERS
                     RALPRYYEAVAATLGCAYLNSQEIVRPSLLDGIHLDAEDHEKLGLAIAAAVRKM"
     gene            complement(2218..>3645)
                     /locus_tag="PLP58_21725"
     CDS             complement(2218..>3645)
                     /locus_tag="PLP58_21725"
                     /inference="COORDINATES: protein motif:HMM:NF015155.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HRJ11216.1"
                     /translation="RRLAAPGILAGAATTAAVFLGLTLCSMPGMAEMGLLVALGVLSG
                     LVVMIGLMPVICAGSPVPQDRIIQAPYSGLVSHRLAAAATGLLVGGVGGVLALLGAPE
                     MERKVDALRPRNSEAMAAFQWVQERLGRSAEASVPVLLTGPMTEMKARAVALEARLKD
                     AVQKGILVRQAVPLPLVFDAGAQRANQAAIEWLLGERARLEAAIGEAGFADAALGLLR
                     GLCAVWEKIPAWPQMPADSAAVPVLGRILSAADGVNGLPEGGGILLSSVSVPGMPGHP
                     DRAKLEELRVHLLPQEGSFVAGWEMLSTTLSRLAQADLAVLLPPVVLLLLVTLFIVFR
                     NLRDVLLSVLSLCAGVAALMATMSLLGQSWNLASLAAIPLLLGTGIDYSIHILLALRR
                     TGNDIRLVRATTGRAVFFAGMTTVIGFSSLVFANNRGLSALGLACCAGVLWVLLIVLW
                     LMPHWRAWLVKSGAASGLAESSSAK"
BASE COUNT          661 a         1205 c         1193 g          586 t
ORIGIN      
        1 tcgacgagat gcatgagcat cgcacgcagc acggggccgc gatcatcgag cgtgttgcgc
       61 ggctgctggc agatgccggg cgtgccgtcg ctcttgccga ggtgctgcgc accggccacg
      121 agatacacac gcacgtcgtc gggcagggtg aggtcggttt ggccttccac atcggtgtgc
      181 agcagcgagg cggcgcggct ccagtattcg gtggaggatt gcgtgaagag gatgcgcggc
      241 gtgtggccgg acttccgcga acgggcgagc gagtcgccgg attcaccggt gacgggatct
      301 gtttgaggca acggggcgag cgggaagaac tcgctgccgg acagatggcc ctcgtgctgc
      361 gtgccatact ccgtgctcat gcggaagcga tggttgaaca tgcctttgcc cgaccccgcg
      421 acgtggatca aggcgccgtc gaagacgatg cggccctgtt catcgccatt caggccttcg
      481 tagaggaagt gatgaatgac gcgacccgac tgcgaaatgc cgaagacgca ggctttctgc
      541 accgcgcctg ccagcggatt cgttttggca tcgccgtgac gaaagaacgc cacgcaatcc
      601 cgcatcgccg tgaggccgag accggtcacg cgaggctcct tcgcggtgta agcgaggtcg
      661 tagagccagc cggggcgtaa accggccttc acatagacat gcgtggcatc gggaatgagc
      721 ttctcatttt cccaacgtcc aaacgcccac tcatcacgcg gcacctcaac accgcccgca
      781 acgcgatgcg gccgcatcgt gagcgtggcg tcggtgttgt cgagactcac ggatggaaac
      841 gccgcgccga tgccccacgg gctccacgag aaggcacggc tgaagacttt ttccgtgctc
      901 gtgatctcca gatgcgcttt gccggtgatt gtcttgccgt tttccgtcgc aatgggcaga
      961 ccgcacagca ggcgctgcgt gtcatccgcc tgcacctccc cattccatcc gcaccacagc
     1021 acggagaagc catgcttcat gagaaagcca tgacccgcat gcgcctccgt cttcggatca
     1081 ttggtgcgct cgccatcgtt gaaggtccac agcgcgagca tgttgccgcg attgttcacg
     1141 tcgtagagca gcacgccgtt gcccttggtt gcatcgaccg gctttagcag aaagaagtcc
     1201 gtccagctct ccaccttgtc acgagcattg cgcggtgccc gctggaggtc gctgatgcgt
     1261 tcgttagcct ggttcgccgg atcggtctcg atgaacatcc ggcccttgag gcgctcgtag
     1321 gctccagcgt tgccaaagac cctgccatcc gcgaagggcg cgcgttccgt gatctcgata
     1381 cgcgcgattt ccgcgcgcgc gccggagcaa aggaatgtcg caagcagaag gcgtggaagt
     1441 gctgtcatgg agaaagtgaa cggcgggaat cggcgggctt ttgccccggc gatgcttgcg
     1501 aacacacggg ttgccgttct tcccttctga tttatgggat ggattccaga tcaaatccgc
     1561 acttccatga aaacactcct ctgcttcggc gactcgaaca cctggggctt ccgcccggac
     1621 agcatcaatg ctccgcatcc cgagcgccac gcgcttgatg ttcgctggac gggagtgctg
     1681 gcgcgggagt tgggaccgga gtggcgcgtc atcgaggagg ggcagaacgg gcgcaccacc
     1741 gtgcatgacg atccgctcaa tgtggcgcgc aacggcaaaa cttatctgcc cgccagcttg
     1801 gaaagccaca tgccggttga cctcgtcatc atgatgctgg gcacgaacga cctgaaggct
     1861 ctccacaact tgccgccggg cgagattgcc gccggtgccg ccgtgctggc gcgcatgatc
     1921 ctgggcagcg ccgccggacc ggggcatcgc gcgccgcgtc tgcttctggt cagcccgccg
     1981 gccatcgggg atctgacgca tttgcccgat ctggcggcga agattcccaa cggcatcgag
     2041 cgcagccgcg ccctgccgcg ttattacgag gcggtggccg ccacccttgg ctgcgcgtac
     2101 ttgaacagcc aggaaatcgt gcggccaagc cttttggacg gcattcatct ggatgcggaa
     2161 gaccacgaaa agctcggcct cgccattgcc gctgcggtgc ggaaaatgtg aggggtgtca
     2221 tttcgcgctg gacgactctg caagccccga tgcggcgccg cttttcacca gccaggcgcg
     2281 ccagtgcggc atgagccaga gcacaataag cagcacccag agcacgcccg cgcagcatgc
     2341 caagcccagg gcggaaaggc cgcggttgtt ggcaaagacg aggctggaga aaccgatgac
     2401 ggtggtcatg cccgcgaaga agacggcccg ccccgtggtg gcgcgcacga ggcggatgtc
     2461 attgcccgtg cgccgcagcg ccagcaggat gtggatgctg tagtcaatgc cggtgcccag
     2521 cagcagcggg atggcggcca ggctggcaag gttccatgac tgtcccagca ggctcatggt
     2581 ggccatcagc gccgccacgc ccgcgcacag ggagagcacg gagagcagca catcccgcag
     2641 attgcggaag acgatgaaga gcgtgacgag cagaagaagg acgaccggcg gcagcagcac
     2701 cgcgaggtcc gcctgggcaa gccgcgaaag cgtggtgctg agcatctccc acccggccac
     2761 gaagctgccc tcctgcggca gcagatgcac gcgcagctcc tccagcttcg cccggtccgg
     2821 gtgtcctggc attccaggca cgctcaccga ggaaagcagg atgccgccgc cctcgggcag
     2881 tccattgaca ccatccgcgg cggagaggat gcggcccagc acgggcacgg cggcgctgtc
     2941 ggcgggcatt tgcggccagg cggggatttt ctcccacacc gcgcacaagc cgcgcagcag
     3001 ccccagggcg gcatcggcga agcccgcctc gccaatggcc gcctccagcc gcgcccgctc
     3061 tccgagcagc cactctatgg cggcctggtt cgcgcgctgc gccccggcgt cgaagaccag
     3121 cggcagcggc acggcctggc gcacgaggat gcctttttgc acggcgtctt tcaggcgcgc
     3181 ctccagcgcc acagcgcgcg ccttcatctc ggtcatgggg ccggtgagca gcacgggcac
     3241 gctggcctcc gccgagcggc ccagtctttc ctgcacccat tgaaaagccg ccatggcctc
     3301 gctgttgcgc gggcgcaggg catccacctt gcgctccatc tcaggcgcgc ccagcagggc
     3361 cagcacgccg cccacgccgc ccacgagaag cccggtggcc gcggcggcaa ggcggtgact
     3421 caccaggccg ctgtacgggg cttgaatgat ccggtcctgc ggcaccgggg agcccgcgca
     3481 gatcactggc atgagaccga tcatgaccac cagaccggag agaacgccca gcgccaccag
     3541 cagccccatc tccgccatgc ccggcatgct gcaaagtgtg agcccgagaa aaacggccgc
     3601 cgtggtggcc gcgcccgcca ggatgcccgg cgcggccaac cggcg
//
LOCUS       DAOMYJ010000363         3624 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Prosthecobacter sp. isolate AMR_MDS_5629
            NODE_9212_length_3624_cov_20.237041, whole genome shotgun sequence.
ACCESSION   DAOMYJ010000363 DAOMYJ010000000
VERSION     DAOMYJ010000363.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867850
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prosthecobacter sp. (wastewater metagenome)
  ORGANISM  Prosthecobacter sp.
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Verrucomicrobiaceae; Prosthecobacter.
REFERENCE   1  (bases 1 to 3624)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3624)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.30
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:34:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,742
            CDSs (total)                      :: 4,684
            Genes (coding)                    :: 4,676
            CDSs (with protein)               :: 4,676
            Genes (RNA)                       :: 58
            tRNAs                             :: 55
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3624
                     /organism="Prosthecobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9212_length_3624_cov_20.237041"
                     /isolate="AMR_MDS_5629"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1965333"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(49..1812)
                     /gene="lnt"
                     /locus_tag="PLP58_21730"
     CDS             complement(49..1812)
                     /gene="lnt"
                     /locus_tag="PLP58_21730"
                     /inference="COORDINATES: protein motif:HMM:TIGR00546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006497 - protein lipidation [Evidence
                     IEA];
                     GO_process: GO:0042158 - lipoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="apolipoprotein N-acyltransferase"
                     /protein_id="HRJ11217.1"
                     /translation="MIKYFPHSSLAPPLALNNPRATVRRTMIRRIFSPRLLPPLLGGV
                     LLSFAYPGWDIQAAVWIWLFPVLWVLWPVNDMAQAPAGRRAFAIGMAAGLGFWIPNLT
                     WLRHSSRVISGALDDTWIGWGPELLGAGAVFGLALVCSLYWGLWAWFSARFARPDPAV
                     LAGASWQSSTWHSLSRAFLAAAAWTGCEWLRGTTLFNGFGWNGLGVALHENRVLIQAA
                     DIIGVTGLSYLPVFIACTAWNTLTRVVRVYQGAGSCRSRLDFTLAMLLLLGAAGYGIQ
                     RLGQPKGETVTVRAVLVQPNVAQVDAWTGRVDEQTYSRLHDFTRLYGEARDGVTHTDL
                     VIWPESALPRHFSEDAEGHAAFFDSLLKAGDFSLLTGTEMHDEQSSHVSALLMRGSAA
                     NRQQYDKVHLVPFGEYLPFRTIPPFSWLRGVLPGDFDPGVKTEPLRLEKPAVEIIPLI
                     CFEDTVGRLARRFARPGPQMIVNLTNDGWFLQSRETAVHLANAKFRAIELRRPMVRAA
                     NTGVTCFIDTLGRVTSRLTDPETGGTFLEGCLPGEAHVPAQGEMTFYARHGDAFSILM
                     LLACAAAAGIERWRSPRGVKK"
     gene            2202..3104
                     /locus_tag="PLP58_21735"
     CDS             2202..3104
                     /locus_tag="PLP58_21735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ11218.1"
                     /translation="MDGKILAYIFLLAAVVAATYGVYQTTLIDDAQRELNVLQAQVES
                     TASAMQQMSRTLELRKQEQAREEEQGKKMEHLQKEQETAKTDVEKAETDLKTLIAEHG
                     KVRAEFERDIARAREETVGMEFFEMELANGSVLKNAKIQRVEEAVLTILHSQGISKIP
                     VNDVHGKLKDRLRFGPEFALPELGPKPAPPKAKAEPQQSASPSPGPAPEKTTADKETE
                     AKEKRILEGKGALARLKREISNMNYEVFKLNEELENRSTSAARRYHATQRKTALEQQL
                     DASRRQLAVVESELAKLGVSVTLY"
     gene            3262..>3624
                     /locus_tag="PLP58_21740"
     CDS             3262..>3624
                     /locus_tag="PLP58_21740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ11219.1"
                     /translation="MSDFPFDQLWTQIRRKTWMWLFQRRQIENAARLVKVFTEIRAVT
                     LAGASEVEISGAAGRGEMTHDEPRVRLRLTDNGDLHVTTTCTCFAGSFCDHAAALLTL
                     LGEPPVQSLVKVMAEPGRG"
BASE COUNT          776 a         1070 c         1180 g          598 t
ORIGIN      
        1 cggctgcgcg cgcgctgagc aggcggggcg cttaagagcg cgcggtttct atttcttgac
       61 gccgcgcggg cttctccagc gctcgatgcc ggcggcagcc gcgcaggcca ggagcatgag
      121 gatggagaag gcgtcgccgt ggcgggcata gaaggtcatt tctccctgag cgggcacatg
      181 cgcctcaccg ggcaggcagc cctccaaaaa ggtgccgccg gtttcggggt cggtgagtcg
      241 agaggtgacg cggcccaggg tgtctatgaa gcaggtgacg ccggtgttgg cggcgcgcac
      301 catcgggcgg cgcagctcga tggcgcggaa tttggcattg gcgaggtgga cggcggtctc
      361 gcggctttgc aggaaccagc cgtcattggt gaggttcacg atcatttgcg gacctggacg
      421 cgcgaagcgc cgcgccagcc tgccgacagt gtcctcaaag cagatgagcg ggatgatctc
      481 cacagccggt ttctccagcc gcagcggctc tgttttcacg cctggatcga agtcgccggg
      541 cagcacgccg cgcagccatg aaaaaggggg gatggtgcgg aatggcaggt attcgccaaa
      601 aggcacgaga tgcactttgt cgtattgctg ccggttcgcg gcgctcccgc gcatgagcag
      661 ggctgagaca tggctggact gctcgtcatg catctcggtg ccggtgagaa ggctgaagtc
      721 acccgctttg agcaggctgt caaagaaggc cgcgtgtccc tccgcatcct cgctaaaatg
      781 acgcggcagc gcgctttccg gccagatgac gaggtcggtg tgggtgacgc cgtcccgcgc
      841 ctcgccgtag agacgggtga agtcgtggag gcggctgtag gtctgctcat caacacgtcc
      901 ggtccaggcg tccacctgcg ccacgttcgg ctgcacaagc acggcgcgca cggtcactgt
      961 ctcacccttt ggctggccga gcctttgaat gccgtatcca gcggcgccga gcagcagcag
     1021 catggccagg gtgaagtcga gccgggagcg gcaggagccc gcgccttgat aaacgcggac
     1081 gacgcgcgtc agggtgttcc atgccgtgca ggcgatgaag acgggcaggt acgacagccc
     1141 tgtgacgccg atgatgtcag ccgcctggat gagcacgcgg ttttcatgca gcgccacgcc
     1201 caggccgttc cagccgaatc cattaaagag cgtggtgccg cgcagccact cgcaaccggt
     1261 ccaggcggcc gcggccagga aagcgcggct cagggagtgc caggtgctgc tttgccacga
     1321 tgccccggcg agcacggcgg ggtctggccg ggcaaagcgc gcgctgaacc aggcccacag
     1381 cccccagtag agcgagcaca ccagcgccag cccgaagacc gcgcccgcgc cgagcagctc
     1441 cggcccccag ccaatccagg tgtcgtcgag cgcgccggaa atgacgcgcg aggaatgacg
     1501 cagccaggtc agattgggaa tccagaaacc cagtccggcg gccatgccga tggcgaaagc
     1561 gcgcctgcct gccggggcct gcgccatgtc gttcacgggc cagagcaccc acaacacggg
     1621 gaagagccat atccatacag ccgcctggat gtcccatcca ggataggcga aggacagcag
     1681 caccccgcca agcaaaggcg gaagcaggcg tggtgaaaag atgcggcgga tcatcgtgcg
     1741 gcgcactgtg gcgcgcgggt tgttcagggc aaggggcggg gcgagacttg agtgaggaaa
     1801 gtacttgatc aatctgcggg aaaagcgcaa aactcggttt tatgagtagt tctggcaagt
     1861 taagccgcat atgtcttcgg agcgtttttg gcgcgttggt tctgaccctg gccacagcct
     1921 gcggaaagag ggaggatttg gaggcgaaag cgcaaattgc caaagcggct ctggatgagc
     1981 aacgggcgcg catggatcag ttgcgcaagg agtcagaggt ggtgtttgcc aggaaacggg
     2041 ctccatccac gacacaaaaa gagcaggaac tgcaaaactc atcgagcagg ggaaagcgcg
     2101 aaaagccacg ctggatcgcg agcttgagca ggccagggaa agcctgaaaa aactccaaga
     2161 tgaccatcaa gagtatcgca aaacccattt gaaagcctga tatggacgga aaaattctcg
     2221 cttacatctt cctgctcgcg gctgttgtgg ccgcaactta cggggtctat caaaccacgc
     2281 tcattgacga cgcccagcgc gaactgaatg tcctgcaagc ccaggtcgaa tccaccgcct
     2341 ccgccatgca gcagatgagc cgcactctgg agttgcgcaa gcaggagcag gccagggagg
     2401 aggagcaggg gaaaaaaatg gaacacttgc agaaggagca ggaaaccgcc aagacggatg
     2461 tggagaaagc cgagactgac ttgaaaaccc tcatcgccga gcatggcaaa gttcgcgcgg
     2521 agtttgagag ggacatcgcc cgcgcccgcg aggagaccgt gggcatggag tttttcgaga
     2581 tggaactagc gaatggaagc gtgctcaaga acgccaaaat ccagcgtgtc gaggaggcgg
     2641 ttttgaccat tcttcactcg cagggaattt ccaaaattcc tgtaaatgac gttcatggaa
     2701 agctcaaaga ccgcctgcgc tttggcccgg agttcgcatt gcctgagctc ggccccaaac
     2761 ccgcgccacc caaggccaag gctgaaccgc aacaatcggc gtcgccatcc cccggcccgg
     2821 ctcctgaaaa aacgacggcc gataaggaga cggaagccaa agaaaaacgc attctcgaag
     2881 gcaagggtgc tctagcccgc ctcaagcgtg agatttccaa catgaactac gaagtgttca
     2941 agctgaatga ggaactggag aaccgctcca catccgccgc ccgcaggtat cacgccacgc
     3001 agaggaaaac cgccttggaa cagcaactcg acgcttcacg ccgtcaattg gcggtggtgg
     3061 agtcggaact ggcaaagctc ggtgtgtctg tgactcttta ctgacgcggt aacagctttg
     3121 aatagccggg tcttctcaag gcacgttaat caagaagaca aggctgggtg tcgcttaatt
     3181 gcgcgctctc attccatttg cacgacactc caacccgccc ccggttgctt tcccgccccc
     3241 gcgcgctatg cggggaggta cgtgtctgac tttcccttcg accagctttg gactcagatc
     3301 cgccgcaaga cctggatgtg gcttttccag cggcggcaga tcgagaacgc cgcgcgcctg
     3361 gtgaaggtct tcacggaaat ccgcgccgtg accctggccg gcgcgagcga ggtggagatc
     3421 tcgggcgcgg cggggcgtgg tgaaatgacc catgacgagc cgcgcgtgcg cctgcgcctc
     3481 acggacaatg gcgacctcca tgtcaccacc acctgcacct gcttcgccgg ctcgttttgt
     3541 gatcatgccg ccgccctgct cacgctgctg ggcgagccgc ctgtgcagtc attggtgaaa
     3601 gtcatggccg agccggggcg cggc
//
LOCUS       DAOMYJ010000364         3617 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Prosthecobacter sp. isolate AMR_MDS_5629
            NODE_9231_length_3617_cov_17.711117, whole genome shotgun sequence.
ACCESSION   DAOMYJ010000364 DAOMYJ010000000
VERSION     DAOMYJ010000364.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867850
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prosthecobacter sp. (wastewater metagenome)
  ORGANISM  Prosthecobacter sp.
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Verrucomicrobiaceae; Prosthecobacter.
REFERENCE   1  (bases 1 to 3617)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3617)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.30
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:34:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,742
            CDSs (total)                      :: 4,684
            Genes (coding)                    :: 4,676
            CDSs (with protein)               :: 4,676
            Genes (RNA)                       :: 58
            tRNAs                             :: 55
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3617
                     /organism="Prosthecobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9231_length_3617_cov_17.711117"
                     /isolate="AMR_MDS_5629"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1965333"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            69..1175
                     /locus_tag="PLP58_21745"
     CDS             69..1175
                     /locus_tag="PLP58_21745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ11220.1"
                     /translation="MISRLIPFLPVLFVGAALQAAAPPDDQARFLAGLSSGGAGELAP
                     LEGEAAWQEHAAAFHRAWVDVEDRQLAHIHTWMPLHFPYIQADPSPLFYFFSGPDFLY
                     ASAFFPNASEYILCGREPVGTLPDVAALSAAARAGALRNLRHSLNAILSYSFFITADM
                     KSDLANTQLSGTLPVLYLFLARAGCRITAAELVWLDAAGDPTQEKSKTPGVRIRFNNH
                     RGVAQTLWYFTTDLSNWGIKDNPAFIEFCRQRGQGTALVKAASYLMHLDSFSSARGFL
                     LEHSRHILQDDSGIPWRFFNAENWMTSLHGHYPGPINLFKQHFQRDLDTAFKTQSAGP
                     LPFGAGYQWRPNVSSLIIATARRTVLRAIPVNEE"
     gene            1261..2322
                     /locus_tag="PLP58_21750"
     CDS             1261..2322
                     /locus_tag="PLP58_21750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007417941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ11221.1"
                     /translation="MTPEEAQNQLDQHVRETLEWHFSPETGCPFWLDWAKKNFDPRGK
                     VGGYADIITHFPHFEDEWLRDLQPEVWVPAAFKGKPYNIFETGGTTGMPKQRIGWNDF
                     RVDYEEFSHKLDDAHFPPGQAWIMVGPTGPRRLRLAVEHLANYRGSSCYFVDLDPRFV
                     KKLISNKQFDVARQYMDHVVDQATLLLKHRKISAIFTTPKLLEALAEKLDLYDAGIRG
                     CFVGGTTMTPQYVRFIIEEVLENRIGFYPTYGNTLMGLAASVPLQPEDQFSITYYAPQ
                     PRAVLRVVNPNETSQTVDYDTWGRVELTTLTKEFFMPRFLERDEAMRRRPRAPYAWDG
                     VADVRPFGAMEKTIVEGVY"
     gene            complement(2371..2742)
                     /locus_tag="PLP58_21755"
     CDS             complement(2371..2742)
                     /locus_tag="PLP58_21755"
                     /inference="COORDINATES: protein motif:HMM:NF017496.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Uma2 family endonuclease"
                     /protein_id="HRJ11222.1"
                     /translation="MIPAACFVDRESPVTCARSEPEPDLAVFAGAPEDFRHAHPATAE
                     LVIEIAINTRQRDLDKAAVCAGAGVKEYWLIEPEAGASARFTEPSAEGYGRRDDFAAG
                     DEAVSTVFPGFRFRLAAWLGA"
     gene            complement(2739..2936)
                     /locus_tag="PLP58_21760"
     CDS             complement(2739..2936)
                     /locus_tag="PLP58_21760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ11223.1"
                     /translation="MITVLESPEARQTVHPLSVEFYHEAGRLGLIGEDVELLEGVLFK
                     KMPKSPLHQPWSACCKDCWNL"
     gene            2984..>3617
                     /locus_tag="PLP58_21765"
     CDS             2984..>3617
                     /locus_tag="PLP58_21765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009958012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III polyketide synthase"
                     /protein_id="HRJ11224.1"
                     /translation="MSAHIHHIATRTPGHAYSQACTRDRLKSWTSNPKTRRLIHAVYN
                     RSGIETRHSVSGDFITGADAALFRTAGDGALIPPGTGARNACYARESRKLAVAVARQA
                     LNDAPGFTARDVTHIVFASCTGFVNPGPDYHIIRELGLRESVQRYTLGFMGCCAAFPA
                     LRMAAQFCAADAGAVVLVVCLELCTLHMQVNDAPDSILANSLFADGAAAAI"
BASE COUNT          621 a         1257 c         1099 g          640 t
ORIGIN      
        1 gtggcgggga gttgcagtga acttttttct tctggcaaag ccggggccgt tgctgctgta
       61 ttggccgggt gatttcacgt ctcatcccat ttctgccggt gctgtttgtc ggcgcggcgc
      121 ttcaggctgc cgctccgccg gatgaccagg cgcgtttcct ggccgggctg tcatcgggcg
      181 gcgccggcga gctggcgccg ctggagggcg aggccgcatg gcaggagcac gccgcggctt
      241 ttcacagggc ctgggtggat gtggaggacc ggcaactggc gcacatccac acctggatgc
      301 ctctgcactt cccctacatc caggcggacc caagcccgct gttctatttt ttcagcggcc
      361 ccgatttcct ttacgccagc gcgtttttcc ccaacgcctc cgagtacatt ctgtgcgggc
      421 gcgagcctgt gggcacgctg ccggatgtcg cggcgctctc cgccgccgcg cgcgccggcg
      481 ccttgaggaa cctgcgccac tccttgaatg ccatcctcag ctacagcttc ttcatcaccg
      541 ccgacatgaa gagcgatctg gcgaacaccc agctcagcgg cacgctgccg gtgctgtatc
      601 ttttcctggc gcgcgccggc tgccgcatca ccgcggccga actggtgtgg ctcgacgccg
      661 ccggtgatcc cacgcaggag aaatccaaaa caccgggcgt caggatccgc ttcaacaatc
      721 atcgcggcgt ggcgcagacg ctttggtatt tcacgaccga tctttcgaac tggggcatca
      781 aggacaaccc cgctttcatc gaattctgcc gccagcgcgg gcagggcacg gcgctggtca
      841 aggccgcctc ctatttgatg catctggaca gcttcagcag cgcgcgcggc tttttgctgg
      901 agcactcccg ccacatcctc caggacgaca gcggcatccc ctggcgtttt ttcaacgcgg
      961 agaactggat gacgagcctg cacggccact accccggacc gatcaacctc ttcaagcagc
     1021 acttccagcg cgatctggac acggccttca aaacgcaaag cgcgggtccg ctgcctttcg
     1081 gagcaggcta ccaatggcgg ccgaatgtct cctccctcat catcgccacc gcgcgccgca
     1141 ccgtcctgcg cgccatcccg gtgaacgagg aatgatcccg ctcccgccgc cgggcgtaga
     1201 aagccctggc cggcagcttt cgtttgactg cccctccctc ccaactctca tagccgcccc
     1261 atgacacccg aagaagccca gaatcaactc gatcagcacg tccgcgaaac ccttgaatgg
     1321 cacttctccc ccgagacggg ctgcccgttc tggctcgatt gggcgaagaa aaacttcgac
     1381 ccgcgcggca aggtgggcgg ctacgctgac atcatcaccc atttcccgca tttcgaggac
     1441 gagtggctgc gcgatctcca gccggaagtc tgggtgcccg cagccttcaa aggcaagccc
     1501 tacaatatct tcgagaccgg aggcaccacc ggcatgccca agcagcgcat cggttggaat
     1561 gacttccgcg tggattacga ggagttcagc cacaagctgg acgacgcgca tttcccgccc
     1621 ggccaggcat ggatcatggt cggccccacc ggcccgcggc ggctgcgcct ggccgtggag
     1681 catctggcga actaccgcgg cagctcctgc tacttcgtgg acctcgaccc gcgctttgtg
     1741 aagaagctca tctccaacaa gcagttcgac gtggcgcgcc agtacatgga ccatgtggtg
     1801 gaccaggcca cgcttttgct caagcaccgc aaaatctccg ccatcttcac cacgccgaaa
     1861 ctgctcgaag ccctggcgga aaagctggat ctctatgacg ccggcatccg cggctgcttc
     1921 gtgggcggca ccaccatgac cccgcagtat gtgcgcttca tcatcgagga ggtgctggaa
     1981 aaccgcatcg gcttctaccc cacctatggc aacacgctca tgggcctggc cgccagcgtg
     2041 ccgctgcagc cggaggacca gttttccatc acctactacg ccccgcagcc gcgggctgtg
     2101 ctgcgcgtcg tcaacccgaa cgagacctcc cagaccgtgg actacgacac ctggggccgg
     2161 gtggagctga ccacgctgac gaaggagttc ttcatgcccc gcttcctgga gcgcgacgag
     2221 gccatgcgcc gcagaccgcg cgcgccctac gcctgggacg gcgtcgccga cgtgcgcccc
     2281 ttcggcgcga tggagaagac gattgtcgaa ggtgtgtatt gagcgaagcg ccagtgacac
     2341 cagcccgcgc ctcatgaggc gcgggctttt tcatgccccc agccaggcgg ccaggcggaa
     2401 gcggaatccg ggaaacacgg tgctcaccgc ctcgtcgccg gcggcaaagt cgtcccgccg
     2461 tccataaccc tccgccgaag gctccgtgaa gcgcgcggat gctcccgcct ccggctcgat
     2521 gagccagtat tctttcacac ccgccccggc gcagaccgcc gctttgtcca gatcacgctg
     2581 gcgtgtgttg atggcgatct cgatgaccag ctccgccgtg gccggatgcg cgtggcggaa
     2641 gtcctccggc gcgccggcga acacggcgag gtccggctcc ggctcggagc gggcgcaggt
     2701 gacggggctt tcgcgatcca cgaagcaggc ggcgggaatc acagattcca gcagtctttg
     2761 caacaggcgg accagggttg gtgcagcggt gattttggca tttttttgaa aagcacgccc
     2821 tccagcagct ccacatcctc cccgatgagt ccgagcctgc cggcctcgtg gtaaaactcc
     2881 acgctcaagg gatggacggt ttggcgtgct tccgggcttt ctaaaaccgt aatcatgggc
     2941 acagcttaag cccgcgcttt gcgatcccgc aatccaggaa tccatgtccg cccacatcca
     3001 ccacatcgcc acccggacgc ccggtcatgc ctacagccag gcttgcacgc gcgaccgcct
     3061 gaaaagctgg accagcaatc ccaagacccg gcggctcatc cacgcggttt acaaccgcag
     3121 cggcatcgag acgcgccaca gtgtcagcgg agacttcatc acgggcgccg atgccgcgct
     3181 tttccgaacg gcgggggacg gcgcgctgat cccgccgggc acgggcgcgc gcaacgcctg
     3241 ctacgcgcgc gagtcgcgca agctggcggt cgccgtggcg cggcaggcgc tgaatgacgc
     3301 gccggggttc accgcgcggg atgtcacgca catcgtgttc gcctcatgca ccggctttgt
     3361 gaatccgggg ccggactacc acatcatccg cgagctgggc ctgcgggaga gcgtgcagcg
     3421 ctacacgctc ggcttcatgg gctgctgcgc ggccttcccc gcgctgcgca tggcggcgca
     3481 gttttgcgcg gcggatgccg gcgccgtggt gctcgtggtg tgcctggagc tctgcacgct
     3541 gcacatgcag gtgaatgacg cgccggactc catcctcgcg aactcgctct ttgccgacgg
     3601 cgccgcggcg gccatcg
//
LOCUS       DAOMYJ010000365         3569 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Prosthecobacter sp. isolate AMR_MDS_5629
            NODE_9393_length_3569_cov_15.935970, whole genome shotgun sequence.
ACCESSION   DAOMYJ010000365 DAOMYJ010000000
VERSION     DAOMYJ010000365.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867850
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prosthecobacter sp. (wastewater metagenome)
  ORGANISM  Prosthecobacter sp.
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Verrucomicrobiaceae; Prosthecobacter.
REFERENCE   1  (bases 1 to 3569)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3569)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.30
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:34:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,742
            CDSs (total)                      :: 4,684
            Genes (coding)                    :: 4,676
            CDSs (with protein)               :: 4,676
            Genes (RNA)                       :: 58
            tRNAs                             :: 55
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3569
                     /organism="Prosthecobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9393_length_3569_cov_15.935970"
                     /isolate="AMR_MDS_5629"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1965333"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1437)
                     /locus_tag="PLP58_21770"
     CDS             complement(<1..1437)
                     /locus_tag="PLP58_21770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ11225.1"
                     /translation="MSDLAQFISRIQDSVEPRLRQEAERLADDDAVRALDLFEDEILA
                     EVILDDRRVNVRWARSPEGRWSEESDCEEETLHKLAACAVLEAARRRLARQPEAVAPS
                     AESEEAFQAQIERRLARRLTPDEENYLGKLEKRFERVRQSGGRIYDQDMVRLHSRWSI
                     QSTDPLHLWPETPAGLREFWDYVALALDEKKLPAPAFLRGFTDLAALRGKLAFWRQES
                     TLPAWKERIREVLRSMDSARQPGPLPLDFRLILTPSEARLQARVDGLEGFRNVSVAEL
                     AGSAREHERGALVLPAEAELLLRACLPLPCSGEVCRYDLDTHGAWLGALFQQPALASR
                     LLTLDETPFHRPAEPLHWRSVEDAETRQLRLGLALADGAPPPLPLRVLPGAVMLYLAA
                     DTLFTGPFWFGEGTLCQEPLRIPLGALSTQEGITFLEKLGLPLPAEIAARVRHEALSV
                     GIRASCVARHPGGGTEHAVFQVVATAPDG"
     gene            complement(1541..2683)
                     /locus_tag="PLP58_21775"
     CDS             complement(1541..2683)
                     /locus_tag="PLP58_21775"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HRJ11226.1"
                     /translation="MCSAPDLTFVFPCLNEEKTLGICIERVKASLDPAGIAYEIVVAD
                     NGSTDRSREIAAAAGCRVVPVAARGYGAALKGGIEAALGKYAMFADSDSTYMYEHAAP
                     LHEAAVKNDAGMAIASRMIGEIEPGAMPPLHRHLGTPVLTWLINTLFHGRLTDCNSGF
                     RCVKKEEYLKWGIRSDGMEFASELLIKALKAGTKTVEIPSGLRCGPPDRVAHLRTWRD
                     GMRHLLFILSERPALFEWLGLLLLVPATLLQILSAITGPVALGPFNVFDIHSQALLLM
                     AGILGTQLYIFGCSLHLRGGDKPLRFTEKFIHLDEGTLFFMLLGVFTAFLATVGGLLI
                     IWAGRGFAGLHQANSLLVAVHILGLMAMGGIGLLSIHTLKKARTGA"
     gene            complement(2702..3427)
                     /locus_tag="PLP58_21780"
     CDS             complement(2702..3427)
                     /locus_tag="PLP58_21780"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HRJ11227.1"
                     /translation="MPDAAPSANDPPPRAEFDAFAEDYDQALNRGLKFSGETKEYFAE
                     GRVNWLRARLSAAGHTAERCLDFGCGTGTAFPYLQAGLSLEDYLGYDPSSESVKEAGG
                     LHPRAKFTADAGDVPESAFDLAFTNGVFHHIPPEHRAQAARLVWRALKPGGWFAFWEN
                     NRWNPVVHFMMSRVPFDRDAQMLFPHQARAQLRDAGFQIVLGDSLFVFPGSLKMLRPL
                     EPLLCKLPLGGQYLVLARKPPLA"
BASE COUNT          610 a         1175 c         1258 g          526 t
ORIGIN      
        1 gccgtccggc gcggtcgcca cgacctgaaa tacagcgtgc tccgtgccgc cgccgggatg
       61 ccgcgcgaca cacgaggcgc ggatgcccac ggagagcgcc tcgtgcctca cgcgcgcggc
      121 gatctccgcc ggcaggggca ggccgagctt ttccagaaaa gtgatgccct cctgcgtgga
      181 caatgcgccc agcgggatgc gcagcggctc ctggcagagc gtcccctcgc cgaaccagaa
      241 cggccccgtg aacaaagtgt ccgccgccaa ataaagcatg accgcgccgg gcaggacgcg
      301 cagcggcagg ggcggcggcg cgccatccgc cagcgcaagg ccgagcctga gctggcgcgt
      361 ctccgcgtcc tccacactcc gccagtgcaa aggctccgca ggacggtgaa aaggtgtttc
      421 gtccagggtc agcaggcgcg aggccagcgc cggctgctgg aagagcgcgc cgagccacgc
      481 gccgtgagtg tcgagatcgt agcggcatac ctcgccggag caaggcagcg gcaggcaggc
      541 gcgcagcagc agctccgcct cggcaggcag gacgagggcg ccgcgctcat gctcgcgcgc
      601 gctgcccgcc agctctgcca cactgacatt gcggaagccc tccaggccgt ccacccgcgc
      661 ctgcagcctg gcctccgagg gtgtgaggat gaggcggaag tccagcggaa gcggccccgg
      721 ctgccgcgcc gagtccatgc tgcgcagcac ctcgcggatg cgctccttcc atgccggcag
      781 ggtgctttcc tgcctccaaa acgccagctt tccacgcaga gcggccaagt ccgtgaagcc
      841 gcgcagaaaa gcgggcgccg gcagcttttt ctcgtccagc gccagcgcca catagtccca
      901 aaactcccgc agccccgcgg gcgtctccgg ccagagatgc agcgggtccg tgctctggat
      961 gctccagcgc gaatgcaggc gcaccatgtc ctggtcgtag atgcgcccgc cgctctggcg
     1021 cacgcgctcg aagcgcttct ccagcttgcc gaggtagttc tcctcatccg gcgtgagcct
     1081 gcgcgccagc cgccgctcga tctgcgcctg aaaagcctcc tccgactccg ccgatggcgc
     1141 cactgcctcc ggctggcggg ccagccgccg ccgcgcggcc tccagcaccg cgcaggcggc
     1201 caacttgtgc agggtctcct cctcgcagtc gctctcctcg ctccaacggc cctccgggga
     1261 cctggcccag cgcacgttca cgcgccggtc gtcgaggatg acctcggcca ggatttcatc
     1321 ctcgaagaga tccaaggcgc gcaccgcgtc atcatcagcc agacgctccg cctcctgccg
     1381 caggcgcggc tccacggagt cctgaatgcg tgaaatgaat tgggctagat cagacatcgc
     1441 tgggggatgc tccaccgcgc gggacggggg gaactggatg ccctagcaca cccccgccgc
     1501 ctttgccaat cctgtctcga aaatgtcacg ctccgcgcgc tcaggcgccg gtgcgggctt
     1561 ttttcaaagt gtggatggag agcaggccga tgccgcccat ggccatgagg ccgaggatgt
     1621 ggacggcgac gaggaggctg ttggcctggt gcagaccggc gaagccgcgc ccggcccaga
     1681 tgatgagcag gccgccgacg gtggcgagga aggcggtgaa gacgccgagc agcatgaaaa
     1741 aaagcgttcc ctcgtcgagg tggatgaatt tttccgtgaa gcgcagcggc ttgtccccgc
     1801 cgcgcaggtg caggctgcag ccgaagatgt agagctgggt gccgaggatg ccggccatga
     1861 gcaggagggc ctggctgtgg atgtcgaaga cgttgaaggg gccgagcgcc acggggccgg
     1921 tgatggcgga gaggatttgc agcagcgtgg cgggcacgag cagcagcagg ccgagccatt
     1981 cgaaaagcgc gggccgctcg gacaggatga aaagcagatg cctcatgccg tcgcgccagg
     2041 tgcgcaggtg cgccacgcgg tcgggcgggc cgcagcgcag gccggagggg atttccaccg
     2101 ttttggtgcc ggctttgagc gccttgatga gcagctcgga ggcgaactcc atcccgtcgg
     2161 agcggatgcc ccacttgaga tactcctcct ttttcacgca gcggaagcct gagttgcagt
     2221 cggtgagccg gccgtggaaa agcgtgttga tgagccaggt gagcacgggc gtgcccaggt
     2281 ggcggtgcag gggcggcatg gcgccgggct cgatctcgcc gatcatgcgg gaggcgatgg
     2341 ccatgccggc gtcgtttttc accgccgcct catgcagcgg cgcggcgtgc tcgtacatat
     2401 aggtgctgtc ggagtcggcg aacatggcat atttgccaag cgcggcctcg atgccgcctt
     2461 tcaatgccgc gccatacccg cgcgcggcca cgggcaccac gcggcagccg gcggcggcgg
     2521 cgatctcgcg ggagcggtcg gtgctgccgt tgtcggcgac gacgatctca taagcgatgc
     2581 ccgccggatc gagcgaggcc ttcacgcgtt caatgcagat gccgagggtt ttttcctcgt
     2641 tgaggcaggg gaagacgaaa gtcaggtcgg gcgcggaaca catgggcggg aggcggactg
     2701 gctaagcaag gggcggcttt ctggcaagca cgagatactg gccgccgagc ggcagcttgc
     2761 acagcagcgg ctccaggggg cgcagcatct tgagcgaacc ggggaagaca aacagcgagt
     2821 cccccagcac gatctggaag ccggcgtcgc gcagctgggc gcgcgcttga tgcgggaaga
     2881 gcatttgcgc gtcgcggtca aagggcacgc ggctcatcat gaaatgcacc accggattcc
     2941 agcggttgtt ctcccaaaag gcgaaccagc cgcccggctt gagggcccgc cagaccaggc
     3001 gcgcggcctg cgcgcggtgc tccggcggga tgtggtggaa gacaccgttg gtgaaggcca
     3061 gatcaaacgc ggactccggc acatcaccgg catcggcggt gaatttcgcc cgcggatgca
     3121 gcccgccggc ttctttgacg gactcggatg aggggtcgta gccgagatag tcctcaaggc
     3181 tcaaacccgc ctgcagatac gggaaggcgg tgccggtgcc gcagccgaag tcaagacagc
     3241 gctccgccgt gtgcccggcg gcggagagcc gcgcgcgcag ccagttcacg cgcccttcgg
     3301 cgaagtattc cttggtctcg ccgctgaact tgaggccgcg gttgagcgcc tggtcgtagt
     3361 cctccgcgaa ggcgtcgaac tccgcccgcg gaggcgggtc gttggcggat ggcgcggcgt
     3421 ctggcatggg cgtgtcagtg cgccggagag gcgctttgcg caagcgcctc gttgagcgcg
     3481 ttttcgtaaa accgcgccac attcggccag gcgcgcgcca tgcccagctc ggagaggcgc
     3541 gcgcgcccgg cgggctggca aaagacgaa
//
LOCUS       DAOMYJ010000366         3567 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Prosthecobacter sp. isolate AMR_MDS_5629
            NODE_9409_length_3567_cov_16.128702, whole genome shotgun sequence.
ACCESSION   DAOMYJ010000366 DAOMYJ010000000
VERSION     DAOMYJ010000366.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867850
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prosthecobacter sp. (wastewater metagenome)
  ORGANISM  Prosthecobacter sp.
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Verrucomicrobiaceae; Prosthecobacter.
REFERENCE   1  (bases 1 to 3567)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3567)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.30
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:34:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,742
            CDSs (total)                      :: 4,684
            Genes (coding)                    :: 4,676
            CDSs (with protein)               :: 4,676
            Genes (RNA)                       :: 58
            tRNAs                             :: 55
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3567
                     /organism="Prosthecobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9409_length_3567_cov_16.128702"
                     /isolate="AMR_MDS_5629"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1965333"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            326..1666
                     /locus_tag="PLP58_21785"
     CDS             326..1666
                     /locus_tag="PLP58_21785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007417429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="POT family MFS transporter"
                     /protein_id="HRJ11228.1"
                     /translation="MAYRTAPAATPLMPPGIPYIIGNELAERFSFYGMRAILYVFMTT
                     ALVTHEGAPDLMSEAEAKKWTHLFIAAVYFTPLIGAFLADLWLGKYRTIIALSLVYCA
                     GHLVLAVDNTRAGLMLGLGLIALGAGGIKPCVSAHVGDQFGRENARLIPRAFNWFYLS
                     INIGAAVSQLLTPVLLESAGPHVAFGVPGLLMLAATLVFWMGRHEFAHIPAEPSRFLD
                     ELCDLRFVRALAGLLPLYLLITVFWSIFDQTASAWVEQAGKMDRVVFGWEIHAAQLQA
                     VNPFFILVLIPIFTLVIYPQAGRLVEVTPLRKIGAGFVLTLATLCVSGWIEGQISAGL
                     RPFIGWQVLAYLLVTSAEILISVTALEFSYTQAPPRMKSVVMSLYLLSMSLGNFFTSR
                     VNAHIESAGADAGLDGAGYYWFFLRLTAAAMVLFFIVAGFYKGRTHIQGEDAPA"
     gene            complement(2059..>3567)
                     /locus_tag="PLP58_21790"
     CDS             complement(2059..>3567)
                     /locus_tag="PLP58_21790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ11229.1"
                     /translation="NLAAAREIARELGGFTLAVESVAIYLGLHPDIRPADYLTRLRAE
                     GLTGVDALPADAGVAAQMQHREKQLRLVLDQTLEKLSPAERTALDYAALLPPESIPWP
                     WLRALVEQEHPDAFATAPGHPDPWPALRRRLEGLRLLTPGDHVNVARLHRMVAAHLRE
                     RLDTHASPAGANPPRAGGAGSEGQRPGPMPAQGSALGSASPRAPSPEGAAQNAHVPPL
                     QGLGEPGAAVPGALPQAGMARPFGAERLRLLRDKVEAFALALQYDIEHDPAAHLWQVA
                     PMQEAVRHWQHGEQDTALGHIAGVVGGIEATLGRLDVALHFLSIYHETAERLLRANPE
                     SAQAARDVSVSLNKLADFLARRGLPGDAEAALGHYQRSLEVLERLLRANPESAQAARD
                     VMVSLERLAAFEAGRSGGEAKALELQSRALGIALKLREGNPQSLFCGRTAAVSFFLTF
                     QRAQAAGNEKLARQCLAGCFSVLDPMVTAGVGLDAPMRQLHAQLAPMFHRDA"
BASE COUNT          503 a         1145 c         1258 g          661 t
ORIGIN      
        1 tggccggagc aggcgctttc cttggagcgg gcggggcggc tttctttgcc ggtggcgcgg
       61 gctttttggc cgggggagcc gcctttttcg gaggggcggg ctttttggag ggtgcggcgg
      121 gctttttggc ggcggaaggt ttcgctgccc ctttttttgg cgtggatttg ctggccatgt
      181 tgcgaatttg tttctgaatt tccggcaggc taacaaggag ttggtgaaag tgtccagtct
      241 ccgctgtgaa aaatgtgcgg ccagagtgat tttttttcac tttcccggct cgacgccccg
      301 cctgcggtgg tcaaaatgcc gcctcatggc ctaccgcacc gcccccgccg ccaccccgct
      361 catgcctccg ggcatcccct acatcatcgg caacgagctg gcggagcgct tcagcttcta
      421 cggcatgcgg gccattcttt acgtcttcat gaccaccgcg ctggtcaccc acgagggcgc
      481 gccggacctg atgagcgagg cggaggcgaa aaaatggacg cacctgttca tcgccgccgt
      541 ctatttcacg cccctcatcg gcgccttcct ggcggacctc tggctgggga aataccgcac
      601 catcatcgcg ctctcccttg tctattgcgc ggggcacctg gtgctggcgg tggacaacac
      661 gcgcgccggt ctcatgctcg gcctgggcct catcgccctc ggagcgggcg gcatcaagcc
      721 gtgcgtttcc gcgcatgtgg gcgaccaatt cggcagggaa aacgcgcgcc tcatcccgcg
      781 cgccttcaac tggttttacc tctccatcaa cattggcgcg gcggtttccc agcttctcac
      841 gcccgtgctg ctggagagtg cggggccgca tgtggccttt ggcgtgcccg gcctgctcat
      901 gctggcggcc accttggtct tctggatggg gcggcacgag tttgcccaca tcccggccga
      961 gccgagccgc ttcctggacg agctgtgcga tctgcgcttc gtgcgcgccc tggccgggct
     1021 gctgcccttg tatctgctga tcactgtgtt ctggtccatc ttcgaccaga ccgcctccgc
     1081 ctgggtggag caggcgggga agatggaccg cgtggtcttc ggctgggaaa tccacgccgc
     1141 ccagctccag gcggtgaacc cgtttttcat cctcgtgctc atccctatct tcactctggt
     1201 gatctacccg caggccgggc ggctggtgga ggtgacaccg ctgcgcaaga tcggcgcggg
     1261 cttcgtgctc accctggcca cgctctgcgt ttccggctgg atcgaggggc agatcagcgc
     1321 cggcctgcgg cctttcatcg gctggcaggt gctggcctac ctgctggtga ccagcgccga
     1381 gattctcatc tccgtcaccg ccctggagtt cagctacacc caggcgccgc cgaggatgaa
     1441 atccgtcgtc atgtccctct acctgctctc catgtccctg gggaatttct tcacctcccg
     1501 tgtcaacgcg cacatcgaga gcgccggcgc ggacgccggg ctcgacggcg cggggtatta
     1561 ctggttcttc ctgaggctca ccgccgccgc catggtgctg ttcttcatcg tggccggctt
     1621 ttacaaaggc cgcacccaca tccagggcga ggacgcgcct gcctgagccg ccgcgtgtga
     1681 aagattcatg cggcgcgcgc gccgcgttga caccgcgttt tcaaaaaggc gggcgttccg
     1741 gccaccggcg ggcatggagg gcgcggacag ggccttggaa gccttttgtt gagaatgccg
     1801 gtctgcgatt gagattgtcg agcttacttt gggcaatgtg gggcctccgt ggaggaagac
     1861 gtgagtcttc ccggatcgga cgagcgggga gagtcgctgg cgtggtggtg cgctggtcct
     1921 gggaacgggg caaactgacg tttgcctcta cgggcctccg tggaggaaga cgtgagtctt
     1981 cccggatcgg gcgcgcgggg agagtcgctg gcgtggtggt gcgctggtcc tgggaacggg
     2041 gcaaactgac gtttgcctct acgcgtcgcg gtggaacatc ggcgcgagct gtgcgtggag
     2101 ctggcgcatg ggcgcgtcca acccgacccc cgccgtcacc atcggatcca gcaccgagaa
     2161 gcagccggcc aggcattgcc gcgccagttt ctcgttcccc gccgcctggg cgcgctggaa
     2221 ggtgagaaag aacgacaccg ccgccgtgcg accgcaaaaa agcgactgcg gattgccttc
     2281 gcgcagcttg agcgcgattc ctaaagcccg gctctggagt tccagcgcct ttgcttcccc
     2341 gcccgaccgc ccagcctcga atgccgccaa ccgttcaaga gaaaccatca cgtcacgcgc
     2401 ggcctgcgca ctctcggggt tggcccgcag cagccgttcg agcacctcca gactgcgctg
     2461 gtaatggccc agggcggcct ccgcgtcgcc gggcagcccg cgacgggcga gaaagtccgc
     2521 gagcttgttc aaactcaccg acacgtcacg cgcggcctgg gcactctcgg ggttggcccg
     2581 cagcaggcgt tcggcggtct cgtggtagat ggagagaaaa tgcagggcca catcaagccg
     2641 cccaagggtg gcctcaatgc cgccgacgac tccggcgatg tggcctaggg cggtgtcctg
     2701 ctcgccgtgc tgccagtggc gcacggcttc ctgcatgggg gcgacctgcc agaggtgcgc
     2761 ggcggggtcg tgctcgatgt cgtactggag ggcgagggcg aaagcttcca ccttgtcacg
     2821 caggaggcgg agcctttcag ccccaaaggg gcgggccatg ccagcctggg gcagcgcccc
     2881 aggaacggcc gcccccggtt cccccaagcc ctgaaggggc gggacatgcg cgttttgggc
     2941 cgccccttca gggctgggcg cgcgcgggga cgcggaaccc agggcgctgc cctgggctgg
     3001 catggggccg ggcctttggc cctccgaacc tgcgcctccg gcgcgtggcg ggtttgcgcc
     3061 tgcgggtgac gcatgcgtgt cgagccgctc ccgcagatgc gcggcgacca tgcggtggag
     3121 gcgggcgacg ttcacatggt cgccgggggt gagcaggcgt aggccctcga ggcggcggcg
     3181 cagggcgggc caggggtcgg gatggccggg ggcggtggcg aaggcgtcgg ggtgttcctg
     3241 ctccaccaag gcgcgcagcc agggccaggg gatgctctca ggagggagca gggcggcgta
     3301 gtccagggcg gtgcgctcgg cgggggagag cttttcgagg gtctggtcga gcacgaggcg
     3361 gagctgcttt tcccggtgct gcatctgcgc ggccacgccg gcatcggcgg gcaacgcgtc
     3421 cactccggtg aggccctcgg cgcgcaggcg ggtgaggtag tcggcggggc ggatgtccgg
     3481 gtgcaggccg aggtaaatgg cgacgctctc gacggcgagg gtgaagccgc ccagctcgcg
     3541 ggcgatctcg cgcgcggcgg cgagatt
//
LOCUS       DAOMYJ010000367         3488 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Prosthecobacter sp. isolate AMR_MDS_5629
            NODE_9715_length_3488_cov_20.436644, whole genome shotgun sequence.
ACCESSION   DAOMYJ010000367 DAOMYJ010000000
VERSION     DAOMYJ010000367.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867850
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prosthecobacter sp. (wastewater metagenome)
  ORGANISM  Prosthecobacter sp.
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Verrucomicrobiaceae; Prosthecobacter.
REFERENCE   1  (bases 1 to 3488)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3488)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.30
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:34:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,742
            CDSs (total)                      :: 4,684
            Genes (coding)                    :: 4,676
            CDSs (with protein)               :: 4,676
            Genes (RNA)                       :: 58
            tRNAs                             :: 55
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3488
                     /organism="Prosthecobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9715_length_3488_cov_20.436644"
                     /isolate="AMR_MDS_5629"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1965333"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..286)
                     /locus_tag="PLP58_21795"
     CDS             complement(<1..286)
                     /locus_tag="PLP58_21795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ11230.1"
                     /translation="MKTESLHALIIDHHLGELSPEATELLEHHLAANAQARAEAERIL
                     QSLDATREAVLRHTELAQVPAAVPHTPLIVKKAAPTPWMARAAAVALLAGG"
     gene            complement(293..787)
                     /locus_tag="PLP58_21800"
     CDS             complement(293..787)
                     /locus_tag="PLP58_21800"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HRJ11231.1"
                     /translation="MDTTGHPPLLERLFTESAGDLTRYFARRHGGDEGVQDLVQQTFL
                     EMARGLRDGKTPKCARGYLFGIARHLSQAAWSRGAREKTVPFEAAADVPAPDDDDRVE
                     AARQTIAALQPIQREILDLRFAQGLSYAEIAEALSIPIGTVRSRLHHAVAEVRHRLKH
                     EIDP"
     gene            complement(901..1905)
                     /locus_tag="PLP58_21805"
     CDS             complement(901..1905)
                     /locus_tag="PLP58_21805"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HRJ11232.1"
                     /translation="MPRIILRTLLKMLLVTLGVFVAGVAGLALWAGSEIASPERRGLQ
                     DYHREFLDNPAAHGVGLASFALKDGTPCVMCAPHPGGKLGARGVKVREQLVQRGITLA
                     EPGKITGTLVLAHGRRGRKEDYLLIAERLCAAGFRCLLPDLPAHGDHPTAHAGYGVLE
                     TELPARVLTEAAERFGFEPRPAGLLGISMGGSVVVHSAAQADAPWRALVVISSFDSFT
                     AVVEHQTSKLFGGWSGPAWARMAGAVYRVRSGVALTDIQPGRKAASLGMPALIAHGSA
                     DRVVPVSAGRRLFDALPDGIEKKWIEIPGADHDNVLITDFPIYTEVAEWMLRHVSGAE
                     "
     gene            2304..>3488
                     /locus_tag="PLP58_21810"
     CDS             2304..>3488
                     /locus_tag="PLP58_21810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009958871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1549 domain-containing protein"
                     /protein_id="HRJ11233.1"
                     /translation="MPFVFPQPVRILTLALAASCLTATAVDFRREVQPVLSEHCYHCH
                     GVDEKTRKGGLRLDVREAALKGGKSDGPAITPGKPEESSILARMTAHDADEIMPPPKE
                     KKPVTAAQIETLKKWIAEGAEYAGHWAFESPKIEARNPTNDKHPLDAMVEERLAKENL
                     KLSPPAAPATLARRLWLDLTGLPPTPEEVEAFTADSIRNPQSAIRNLTDTLLQSPRHG
                     EKWARVWLDAARYSDSNGYEKDLPREQWAWRDWVIAALNRDMPYDQFIIEQIAGDLLP
                     GATQEQVIATGFLRNGMVNEEGAIVPEQFRIEGVFDRMDCVGKAVLGLTLQCAQCHTH
                     KFDPITHDEYFGMFAFLNNAYEAQTWVYDEAQQKAVAKVRSGLTSIRDRLKKQTPDWQ
                     EKL"
BASE COUNT          689 a         1200 c         1035 g          564 t
ORIGIN      
        1 cgccgcccgc cagcagcgcg actgccgccg cgcgcgccat ccagggcgtc ggcgcggcct
       61 ttttcacgat gagcggcgtg tgcggcactg ccgccggcac ttgcgccagc tcggtatgcc
      121 gcagcacggc ctcgcgcgtg gcatcaaggg attgcaaaat gcgctccgcc tcggcgcggg
      181 cctgcgcgtt ggccgccaga tgatgctcca gcagctccgt cgcctccggt gaaagctcgc
      241 cgagatgatg gtcaatgatc agtgcgtgaa gggactcggt tttcatgggg attcaggggt
      301 caatttcatg tttcaaacga tggcgcacct ccgccacggc gtgatgcaaa cgcgagcgca
      361 cggtgccgat gggaatgctc aaagcttcgg cgatctccgc gtaggacagt ccctgtgcga
      421 aacgcaggtc cagaatctcg cgctggatcg gctgcagcgc cgcgatggtc tgccgcgccg
      481 cttccacacg gtcgtcgtcg tccggcgcgg gaacatccgc cgcagcctca aatggcaccg
      541 tcttttcacg cgcgccgcgc gaccacgccg cctggctcag atgccgcgcg atgccgaaca
      601 aatagccgcg cgcgcatttg ggagtcttcc catcgcgcag cccgcgcgcc atttcgagaa
      661 aggtctgctg caccagatcc tgcacgcctt cgtcaccgcc atgccgccgc gcgaagtagc
      721 gcgtcagatc ccccgcgctc tccgtgaaga ggcgttcgag caggggagga tgtccggtgg
      781 tgtccacgag gttggatagc cattcggcgg ctgccagcgc agcctaccat tcgagacgtg
      841 actttgacga tgcaaaaggt tcacaggaaa tttaacgacc cgggccgacc cttatcaaac
      901 tcactccgct ccgctcacat ggcgcagcat ccactccgcc acctcggtat agatggggaa
      961 gtcggtgatg agcacattgt catgatcagc gcctgggatc tcgatccact ttttctcaat
     1021 cccgtcgggt agcgcgtcaa agagacgccg cccggcggac acgggcacca cgcggtcggc
     1081 gctgccatgc gcgatgagcg ctggcatgcc cagggatgcc gctttccgcc ccggctggat
     1141 gtccgtcagg gcgacacccg accgcactcg atagaccgcg cccgccatcc gcgcccaggc
     1201 cgggccgctc catccgccga acagtttcga agtctgatgc tccaccactg cggtgaagga
     1261 gtcgaagctg gaaatgacca ccagcgcgcg ccagggcgcg tccgcctgag cggcggaatg
     1321 caccactacc gagccgccca tggagattcc caacagaccg gcgggccgcg gctcgaagcc
     1381 aaagcgctcc gccgcctctg tcagcacgcg cgcgggcagc tctgtttcaa gcactccata
     1441 acccgcatgg gcggtcgggt ggtcgccatg cgcgggcagg tctggcagca ggcagcggaa
     1501 gccggcggcg cagaggcgct cggcgatgag cagataatcc tccttccgtc cgcgccgccc
     1561 atgcgccagc acgagtgtgc cggtgatctt tccaggctcc gccagcgtga tcccgcgttg
     1621 cacaagctgt tcccgcactt tcacaccgcg cgcgccgagt tttccgccgg gatgaggcgc
     1681 gcacatcaca cagggtgtgc cgtccttcag ggcaaaggag gcaagcccga ccccgtgcgc
     1741 ggctggatta tccagaaact cacggtgata atcctgcaat ccccgcctct ccggactcgc
     1801 gatctccgac cccgcccaca aagccaggcc cgccaccccg gcaacgaaga caccgagagt
     1861 gacaagcagc attttgagca gggtacgaag gatgatccgg ggcatgaccg gccgaggaat
     1921 ccgccctctc caccgactcg gcaagccatt tcaaaggaga ccgggacaat gaagcgccga
     1981 acacagccat gagcacagcc tgcgcgccgc tttcccgcag tgttgaggta tgaagaccgg
     2041 ctccatcaaa acaaacttga gtcggggtgg cagtcctcat cgccggattg acatgtctga
     2101 tacgcaggag gcttgtttga taagatggaa atcctgttct gcgcactgga tggcctgctc
     2161 gtgaggggga actcgatgct cgaaaacctc ccttcaggcc tcgcacgacc tccacccttg
     2221 tccaatcatg cccgcgcttt gtctggatgc cgcaggctgg ccgggccgcg tcagaagtca
     2281 tgcccgcgcg cgttggtttg cagatgccat tcgttttccc acaacccgtc cggatcctca
     2341 cgcttgccct ggccgcctcc tgcctgactg cgacggccgt ggacttccgc cgcgaggtgc
     2401 agccggtcct ttccgagcac tgttaccact gccacggcgt ggatgagaag acgcgcaaag
     2461 gcggcctgcg cctcgatgtg cgcgaggccg cgctgaaggg cggcaagagc gacggaccgg
     2521 ccatcacccc cggcaaaccg gaggaaagct ccatcctcgc ccgcatgacg gcccatgacg
     2581 ccgatgagat catgccgccg ccgaaggaga aaaagcccgt cacagccgcg cagatcgaga
     2641 ctttgaaaaa atggatcgcc gagggtgccg agtacgccgg gcactgggct ttcgaaagtc
     2701 cgaaaatcga agctcgaaat ccgacgaacg acaagcaccc gttggatgcc atggtcgaag
     2761 agcgactcgc aaaggaaaac ctcaaactct ctccgcccgc cgcccccgcc acccttgccc
     2821 gacgcctctg gctcgacctc accggcctgc cgccgacccc cgaggaagtc gaggcattca
     2881 cagcggactc aatccgcaat ccacaatccg caatccgcaa cctcaccgac accctcctcc
     2941 aaagcccgcg ccacggcgaa aaatgggccc gcgtctggct cgacgccgcc cgttactccg
     3001 acagcaacgg ttatgaaaaa gacctgccgc gcgagcagtg ggcctggcgg gactgggtga
     3061 tcgccgcctt gaaccgcgac atgccttacg accagttcat cattgagcag atcgccggcg
     3121 atttgctccc aggcgccacg caggagcagg tcatcgccac cggatttctg cgcaacggca
     3181 tggtcaatga ggagggagcc atcgtgcccg agcagttccg catcgaaggc gtcttcgacc
     3241 gcatggactg cgtgggcaaa gccgtgctcg gcctcactct gcaatgcgcc cagtgccaca
     3301 cgcacaagtt cgatcccatc acgcatgacg aatacttcgg catgttcgcc ttcctcaaca
     3361 acgcctacga ggcgcagacc tgggtctatg atgaagcgca gcagaaggcc gtcgccaaag
     3421 tccgctccgg cctcaccagc atccgggacc ggctgaaaaa gcaaaccccc gactggcagg
     3481 aaaagctg
//
LOCUS       DAOMYJ010000368         3480 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Prosthecobacter sp. isolate AMR_MDS_5629
            NODE_9742_length_3480_cov_16.804672, whole genome shotgun sequence.
ACCESSION   DAOMYJ010000368 DAOMYJ010000000
VERSION     DAOMYJ010000368.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867850
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prosthecobacter sp. (wastewater metagenome)
  ORGANISM  Prosthecobacter sp.
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Verrucomicrobiaceae; Prosthecobacter.
REFERENCE   1  (bases 1 to 3480)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3480)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.30
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:34:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,742
            CDSs (total)                      :: 4,684
            Genes (coding)                    :: 4,676
            CDSs (with protein)               :: 4,676
            Genes (RNA)                       :: 58
            tRNAs                             :: 55
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3480
                     /organism="Prosthecobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9742_length_3480_cov_16.804672"
                     /isolate="AMR_MDS_5629"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1965333"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1451
                     /locus_tag="PLP58_21815"
     CDS             <1..1451
                     /locus_tag="PLP58_21815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ11234.1"
                     /translation="GSGTVLPERRGGGAPSHSSLIPGAPALPTLGGGHEGREAGSLLG
                     RGVPQGMPVMQPAPAMPASQPLHQPMPRGSRPLRRPKRASNIFMFSLAVIFLLGTLAA
                     AGWMFREPLMEIVHKYMPAGNETPVEVPVPSPKPVVSTPGPGPEPETVNAPAPKVAAE
                     PPPEKPAITGFDPDEPAPPKAVPLTDDERTALTAGPGMSNSQPPAPAVPDATPGSGLQ
                     EVPQPAPDTKSGVARADIEIEVPEEAKPAAEALMKFLTARTLEDRLRHSLAPDFMRPL
                     MERYYKGAPEGPVKVDAVGLVRFDPKPQIGGGAHAVFGLESREWEFPIPVMLEEGKDG
                     FRVDWLSFVEFKDRLLERFFENYQEGPARFHVGITRTHYFDDKVPNSGSKDAFLVSPA
                     PPNPWKTTVFLDKDSPLARDLRERIPWGAQVWAVVELEWVKLGATQWVQLAAVPQLNW
                     YSVPAANGQAGGGPSSQNQLPNEIQRAAPVGR"
     gene            complement(1462..2664)
                     /locus_tag="PLP58_21820"
     CDS             complement(1462..2664)
                     /locus_tag="PLP58_21820"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HRJ11235.1"
                     /translation="MFGERWQKILDAQGDDIALRHPGGVWSFHELEKQARTLEPDHCP
                     VLGPYYLAQGNGPEIARALLAGFLSGLPVQVVEKDRSRRVPSCPAPAGTALIKQAVGA
                     SGSRRCQFFTLAQAAADADRLHAALGLGGIGTALAPLSVAHSFGLTTTLLPMLLHGLR
                     VHWLPTPFPAGIAEALALHERVFLPGVPAMWKAWLLSGLDLERVALAVSAGSPLTLEL
                     ESKVRRQFGLKLHNLYGTSETGAISYDAGQSPRTDARDLGRLLPGVRALCDEQGFLIV
                     ESDAAGLGYDAPLPGELFSASRHRTWDKARLEGDALFFESCAGPGINVASRKLSPDEI
                     AAKIRRATGIADIAVHGAASRDPERVQDVVAVAGLPAGQITQAFKSAACAGLAAWEVP
                     RHWRGKPG"
     gene            2780..>3480
                     /locus_tag="PLP58_21825"
     CDS             2780..>3480
                     /locus_tag="PLP58_21825"
                     /inference="COORDINATES: protein motif:HMM:NF012337.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl synthase N-terminal-like
                     domain-containing protein"
                     /protein_id="HRJ11236.1"
                     /translation="MSRPSDPRILITGAGIVSPLGLDWRENEASLMADRAAFRPVTLF
                     DVSERIAKTAASVDLPEDRLFQRVPRRRAHLVDRGTKMLMLAVREALGMAGMAGLAGV
                     DAIVLGTSAGAMSVGQEYFRAARASPSRQPGQLSRLEHYQPQRQISLIAAEYDWRGPV
                     ILISNACASGANAIGEAMALIRCGRARRVLAGGYDALAELVFAGFDTLRALAPSGIPR
                     PFDAARDGLALGEGAA"
BASE COUNT          567 a         1147 c         1208 g          558 t
ORIGIN      
        1 gggggagcgg aaccgtgctg cctgaacggc gcggcggcgg cgcgccatcc cacagcagtc
       61 tcatccctgg agcgcccgcg ctgcccacgc taggcggcgg gcatgaaggc agggaagccg
      121 gttccctgct cggtcgcggc gtgccgcagg gcatgcctgt gatgcagccg gccccggcca
      181 tgccggcctc gcagccgctg caccagccca tgccgcgcgg ctcccgtccg ctgcgaaggc
      241 ccaagcgcgc ctcgaacatt ttcatgttca gtctcgcggt cattttcctg ctcggcacgc
      301 tggccgcggc tgggtggatg ttccgcgagc ctttgatgga gatcgtccac aagtacatgc
      361 cggcggggaa tgagacgccg gtggaggtcc ccgtgccttc gccaaaaccc gtggtttcca
      421 cacccggtcc aggccctgag ccggagaccg tgaatgcgcc agcgccaaaa gtggcggcgg
      481 agccgccgcc agagaagccg gcaatcaccg gattcgatcc agatgaaccc gcgccgccga
      541 aggctgtgcc gctcactgat gacgagcgca ctgctttgac tgccgggcca gggatgagca
      601 attctcagcc ccctgcgccg gcagtgcccg acgcgacgcc gggttcggga ttgcaggagg
      661 ttcctcagcc ggcgccggac acaaagagcg gcgtggccag ggcggacatt gagatcgagg
      721 tgcccgagga agccaagccc gccgccgagg cgctgatgaa attcctcacc gcgcgcacgt
      781 tggaggatcg tctccgccac agcctggcgc cggatttcat gcgcccgctg atggagcgct
      841 attacaaggg cgcgccggaa ggcccggtga aggtggatgc cgtgggcctg gtgcgctttg
      901 acccgaagcc gcagattggc ggcggcgctc atgccgtgtt cggcctggaa agccgcgagt
      961 gggagtttcc catcccggtg atgctggagg aaggcaagga cggcttccgc gtggactggc
     1021 tgtcgtttgt ggagttcaag gacaggctgc tggagcgctt ctttgaaaac taccaggaag
     1081 ggccggcgcg cttccatgtg ggcatcacgc gcacgcatta ctttgacgac aaagtgccga
     1141 actcgggttc gaaagacgct tttctcgtca gccccgcgcc gccgaatccc tggaaaacga
     1201 ccgtctttct cgacaaggac tcgcccctgg cgcgcgacct tcgcgagcgc atcccctggg
     1261 gcgcgcaagt gtgggcggtg gtggagttgg agtgggtgaa gctgggcgcc acgcagtggg
     1321 tgcagctcgc cgccgtgccc cagcttaact ggtactcggt gccagccgcc aacggccagg
     1381 ccggcggagg cccatcctcg cagaaccagc ttcccaacga gattcagcgc gcggctccag
     1441 tggggcgctg atgcgcgcgg ctcagccggg tttgccgcgc cagtggcgcg gcacctccca
     1501 ggcggcgagt ccggcgcagg cggcggattt gaaagcctgg gtgatctgcc ccgcgggcag
     1561 gccggcgacg gcgacgacat cctgcacgcg ttcggggtcg cggctggcgg cgccatgcac
     1621 cgcgatgtcc gcgatgccgg tggcgcggcg gatttttgcg gcgatctcgt ccgggctgag
     1681 cttgcgactg gcaacattga tgcccgggcc ggcgcaggat tcgaaaaaca gggcgtcacc
     1741 ctccaggcgc gccttgtccc aggtgcggtg acgacttgcg gaaaaaagct cgcctggcag
     1801 cggtgcatcg tagccgagac cggcggcgtc gctttccacg atgagaaagc cctgctcgtc
     1861 gcacaaggca cggactcccg gcagcaggcg gccgagatcg cgcgcatcgg tgcgcggaga
     1921 ctggccggca tcatagctga tggcacccgt ctcgcttgtg ccgtagagat tgtgcagctt
     1981 gaggccgaac tgccgccgca ctttgctctc aagctccagc gtgagggggg aacccgcgct
     2041 gaccgccagc gccacgcgct ccaggtccag gccggaaagc agccatgcct tccacatcgc
     2101 gggcactccg ggcaggaaca cgcgctcatg cagggccagc gcctcagcga tgccggcggg
     2161 gaaaggcgtg ggcagccagt ggacgcgcag gccatgcagc agcatgggca gaagcgtggt
     2221 ggtcaggccg aaagagtgcg ccacgctcag gggagccagc gccgtgccga tgccgccgag
     2281 tcccagcgcg gcatgaaggc ggtccgcgtc cgcagcagcc tgcgcgaggg tgaagaactg
     2341 gcatctgcgc gatccagagg cgccgacggc ctgcttgatc aaggcggtgc cggcgggagc
     2401 ggggcaggat ggcacgcggc gggagcggtc tttctccacc acctgcaccg gcaggccgga
     2461 aaggaagccc gccagcagcg cgcgggcgat ctccggcccg ttaccctgcg ccaggtaata
     2521 aggtccaagc accggacaat ggtctggctc cagggtgcgg gcctgctttt ccaactcgtg
     2581 aaagctccac acgccgccag gatgccggag ggcgatgtca tcgccctgcg cgtccaggat
     2641 tttttgccag cgttcaccaa acatttgaca ctctccgccg cgaacagcaa aacgtggagc
     2701 gcgggcgtgc cagtgtgcgc gtgtccgtcc tgagttcaag cctgggaatc catccggctg
     2761 aaaacttcgt tttctcattg tgtcccgacc ctccgatcct cgaattctca tcactggcgc
     2821 aggcatcgtc tcgcccctgg ggctggactg gcgggaaaac gaggcgtctt tgatggcgga
     2881 ccgcgccgct tttaggccgg tgacgctttt cgatgtctcc gagcgcatcg cgaaaacagc
     2941 ggccagcgtg gatttgccgg aagaccggct ttttcagaga gtgccgcgcc gccgcgcgca
     3001 tctggtggac cgcggcacaa aaatgctgat gctggccgtt cgcgaggcct tgggcatggc
     3061 cggcatggcg gggttggccg gggtggatgc catcgtgctc ggcacttcgg cgggggccat
     3121 gagcgtgggg caggaatact tccgcgcggc gcgcgccagt ccgtcccgtc agccggggca
     3181 gctctcgcgg ctggagcact accagccgca aagacagatc tccctcatcg ccgccgagta
     3241 cgactggcgc gggccggtca tcctcatttc caatgcctgc gccagcggcg ccaacgccat
     3301 cggcgaggcc atggctctca ttcgctgcgg gcgcgcgcgc cgtgttctgg ccggaggcta
     3361 cgatgccctg gcggagctgg ttttcgcagg cttcgacact ctgcgcgccc tggcgccctc
     3421 cggcattccc cggcccttcg atgccgcgcg cgacggactc gccctcggcg aaggcgccgc
//
LOCUS       DAOMYJ010000369         3476 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Prosthecobacter sp. isolate AMR_MDS_5629
            NODE_9755_length_3476_cov_23.950015, whole genome shotgun sequence.
ACCESSION   DAOMYJ010000369 DAOMYJ010000000
VERSION     DAOMYJ010000369.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867850
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prosthecobacter sp. (wastewater metagenome)
  ORGANISM  Prosthecobacter sp.
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Verrucomicrobiaceae; Prosthecobacter.
REFERENCE   1  (bases 1 to 3476)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3476)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.30
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:34:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,742
            CDSs (total)                      :: 4,684
            Genes (coding)                    :: 4,676
            CDSs (with protein)               :: 4,676
            Genes (RNA)                       :: 58
            tRNAs                             :: 55
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3476
                     /organism="Prosthecobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9755_length_3476_cov_23.950015"
                     /isolate="AMR_MDS_5629"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1965333"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2070
                     /locus_tag="PLP58_21830"
     CDS             <1..2070
                     /locus_tag="PLP58_21830"
                     /inference="COORDINATES: protein motif:HMM:NF019223.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1549 domain-containing protein"
                     /protein_id="HRJ11237.1"
                     /translation="DAETTAPDGNSNFRTWRDQQGRTVEATFRGIENGQIFLQTRNGY
                     VHRLPLNNLSEEDQKLAATLKPEGLGIPKDPNVAQAAAMIDKGVLMGLEKAGQKPNEL
                     ASDEQFIRRVYLDLAGRIPTREETLSFLGDGSASKRAKIIDRLVSSEGFTSRMYNYLS
                     DMLRIADDAQKAKFFHYEEWLKQQIADNRPWNQIVRDMLVADGKLLDNGAAGYLLRDR
                     GMRLDNLSLTLSTFLGADVSCAQCHDHPFADWTQRQFYEMAAFFGATETYTRSQARAS
                     MRGLRDELTQQELQQAKRLFDANALAIFDQKQNDLVLPEDYKYKDASPGDPVKPALIA
                     WGKDDRRLKCYQTAEAALKKAGDDTRLREIFADWMTAPDNPRFAMTIANRLWKMVFGV
                     GVAEPVAELDDPNASSNPALLHHLGREMVRLNFDLRAFMRLLCNTQTYQREAYTAELV
                     MGEPYLFPGPVLRRMTAEQAWDSCVTLAIGERVDAFKLRRADSYKEVMALNVSEISPE
                     IIKEKLGQMQSMRRKADGPLGGGKPGKAGKNRPNRRAMMETASQVNEEGEVTQPDMML
                     GLALARASELRQPEREGHFLRMFGQSDRQIADSNSSEGSVPQVLMLMNGEAQQVLSSR
                     SALAVADAEKHETAQAQVESLYLSFFSRRPTEAELKTVYEEINGGLTPGDLAWVLFNS
                     REFVFVQ"
     gene            2103..3386
                     /locus_tag="PLP58_21835"
     CDS             2103..3386
                     /locus_tag="PLP58_21835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009960473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1501 domain-containing protein"
                     /protein_id="HRJ11238.1"
                     /translation="MKNSLLQLDPQSRRQFAETIAKATLGVTLLPQLSRAADTAPGVD
                     NKMLPGFGNAKHVIWLQMIGGMSHIDTLDPKEGDTKGPSDPVQTKAGFKLGGFLPTLA
                     KEHSEKLAIIRSMTSKTGVHASGQYIIRTGYDPRATIKHPVLGAWAQQLLGKNSGSLP
                     SSVCINRGPDNGNGFFSAAFSPLPIHDPEAGLQYANSGGDTLNKRLALLNRLDSDFRA
                     KFQDTNLKAYTDFYDDTLKLLSSEDLTVFRLSEESSDARSSYGMNKFGQGCLLARRLV
                     ERGVRFIEVAYGGWDMHNDIDDGMEDHGATLDTALSALLTDLQSKGLLESTMVVLCSE
                     FGRSPKINSRNGRDHHPKVFSTLLAGGPVKGGTIYGASDKEGSAPADKQATIQDFHST
                     VGHAMGLDVNEIVMSPSNRPFTVGDKGQVITEVFA"
BASE COUNT          723 a         1236 c          974 g          543 t
ORIGIN      
        1 gacgctgaga cgacagcccc ggatggcaac tccaacttcc gcacttggcg cgaccagcag
       61 ggccgcaccg tggaggccac ttttcgcggc atcgaaaacg ggcagatttt tctgcaaacc
      121 cgcaacggtt atgtccaccg gctgccgctg aacaacctct ccgaagaaga tcaaaagctg
      181 gccgccaccc tgaaacccga ggggctcggc atccccaaag acccaaatgt ggcccaggca
      241 gccgccatga tagacaaagg cgtgctcatg gggctggaga aggccgggca gaagccgaac
      301 gaactggcca gtgacgagca gttcatacgc cgcgtctatc tcgacctcgc cgggcgcatc
      361 cccacgcgcg aggagacgct gtccttcctc ggcgatggga gcgcctcgaa acgcgccaag
      421 atcattgacc ggctggtgag cagcgagggc tttacctcgc gcatgtacaa ctatctgtcc
      481 gacatgctgc gcatcgccga tgacgcgcag aaggccaagt ttttccacta cgaggagtgg
      541 ctcaaacagc agatcgccga caaccgcccc tggaaccaga tcgtgcgcga catgctcgtg
      601 gccgacggca aactactcga caacggcgcc gccggttacc tcctccggga ccgcggcatg
      661 aggctcgaca acctcagcct cacgctctcc acctttctcg gcgcggatgt ctcctgcgcc
      721 cagtgccacg atcacccctt cgccgattgg acgcagcggc agttttatga aatggcagcc
      781 ttttttggcg ccaccgagac ctacacccgc agccaggcgc gcgcctccat gcgcggcttg
      841 cgcgatgagc tgacccagca ggaactgcag caagccaagc ggctgtttga cgcgaacgcg
      901 ctggccattt tcgaccagaa gcaaaacgac ctcgtcctgc ccgaagacta caaatacaag
      961 gacgccagcc ccggcgaccc tgtgaagccc gccctcatcg catggggcaa ggacgaccgc
     1021 cgcctcaaat gctaccagac cgccgaggcc gccctcaaga aagccggcga cgacacgcgc
     1081 ttgcgtgaga ttttcgccga ctggatgacc gcgccggaca acccgcgctt cgccatgacg
     1141 atcgccaacc ggctttggaa gatggtcttt ggtgtcggcg tcgccgagcc cgtcgcggaa
     1201 cttgacgatc ccaacgcctc aagcaatccc gccctgcttc accacctcgg cagggaaatg
     1261 gtgcgcctga acttcgacct ccgcgccttc atgcgcctgc tttgcaacac ccagacctat
     1321 cagcgcgagg cctataccgc ggagctggtg atgggcgagc cttacctttt ccccggcccc
     1381 gtcctgcgcc gcatgaccgc cgagcaggcc tgggactcat gcgtcacact cgccatcggc
     1441 gaaagagtgg acgccttcaa attgcgccga gcggacagct acaaggaggt catggccctg
     1501 aatgtctccg agatcagccc tgagatcatc aaggaaaagc tcggccagat gcagtccatg
     1561 cgccgcaagg ccgacggccc gctgggtgga ggcaagccgg gcaaagcggg gaaaaaccgc
     1621 cccaaccgcc gcgccatgat ggagaccgcc agccaggtca acgaggaggg cgaggtcacc
     1681 cagccggaca tgatgctcgg tctcgcgctc gcccgcgcct ccgaactgcg ccagcccgag
     1741 cgcgaggggc acttcctgcg catgttcggc cagagcgacc gccagatcgc ggacagcaac
     1801 agctccgaag gcagcgtgcc ccaggtgctc atgctcatga acggcgaggc ccagcaagtg
     1861 ctcagcagcc gaagcgccct cgccgttgcc gacgcggaaa agcacgagac cgcacaagcc
     1921 caggtcgagt ccctctatct cagcttcttc tcccgccgcc ccaccgaggc ggaactcaag
     1981 accgtctatg aggagatcaa cggcggcctg actcccggag acctcgcctg ggtgctcttc
     2041 aactcgcgcg agttcgtctt tgtgcagtga ccctccattg aaaccaccag catttcacgg
     2101 ccatgaaaaa ttccctgctc cagctcgacc cgcaatcccg ccgccagttc gccgagacca
     2161 tcgccaaggc aaccctcggt gtgacactgc tcccgcagct ctcccgcgcc gctgacacgg
     2221 ctccaggcgt ggacaacaag atgctgcccg gttttggcaa tgccaaacat gtcatctggc
     2281 tgcaaatgat cggcggcatg tcccacattg acacgctcga ccccaaggag ggcgatacca
     2341 aaggcccctc cgacccggtg cagaccaagg ctggcttcaa gctcggcggc ttcctgccca
     2401 ccctggcgaa ggagcactcc gaaaagctcg ccatcatccg cagcatgacc tccaagacag
     2461 gtgtccatgc ctccggccag tacatcattc gcaccggcta cgacccgcgc gccaccatca
     2521 agcaccccgt gctcggagcc tgggcgcagc agttgctcgg caaaaacagc gggagcctgc
     2581 cctcctccgt ctgcatcaac cgcggcccgg acaacggcaa cggcttcttc tccgccgcct
     2641 tcagtcccct ccccatccac gatcccgaag ccggcctcca gtatgccaac agcggcggcg
     2701 acaccttgaa caagcgcctg gccctgctca accggctgga ctcggacttt cgcgccaaat
     2761 tccaggacac gaacctgaaa gcctacaccg acttttatga cgacaccctg aagctgctct
     2821 ccagcgagga cctgaccgtc ttccggctct ccgaggaaag cagcgacgcg cggtcctcct
     2881 atggcatgaa caaattcgga cagggctgcc tgctcgcgcg caggctcgtc gagcgtggcg
     2941 tgcgcttcat cgaggtggcc tacggcggct gggacatgca caacgacatt gacgacggca
     3001 tggaggacca cggcgccacg ctcgacaccg ccctctccgc actgctcacc gatcttcaat
     3061 ccaaaggcct gctggagtcc acgatggtcg tcctgtgctc tgaattcggc cgctcgccga
     3121 aaatcaacag ccgcaacggc cgcgaccacc acccgaaagt cttcagcacc ctgctcgctg
     3181 gcggcccggt caaaggcggc accatctatg gtgccagtga caaagaaggc agcgcccccg
     3241 ccgacaagca ggccaccatc caggacttcc acagcaccgt cggccatgcc atgggcctcg
     3301 acgtgaacga aatcgtcatg tcccccagca accgcccctt caccgtcggc gacaaaggcc
     3361 aggtcatcac ggaagtgttc gcgtgatttt gacgtgaagc gccagcggtt gtctagccgg
     3421 cgggtgcgtc gccagaatga tgtaggccgg gtgtggcgca gcccgcccgg cttttg
//
LOCUS       DAOMYJ010000370         3455 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Prosthecobacter sp. isolate AMR_MDS_5629
            NODE_9814_length_3455_cov_16.862647, whole genome shotgun sequence.
ACCESSION   DAOMYJ010000370 DAOMYJ010000000
VERSION     DAOMYJ010000370.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867850
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prosthecobacter sp. (wastewater metagenome)
  ORGANISM  Prosthecobacter sp.
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Verrucomicrobiaceae; Prosthecobacter.
REFERENCE   1  (bases 1 to 3455)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3455)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.30
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:34:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,742
            CDSs (total)                      :: 4,684
            Genes (coding)                    :: 4,676
            CDSs (with protein)               :: 4,676
            Genes (RNA)                       :: 58
            tRNAs                             :: 55
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3455
                     /organism="Prosthecobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9814_length_3455_cov_16.862647"
                     /isolate="AMR_MDS_5629"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1965333"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            114..1460
                     /locus_tag="PLP58_21840"
     CDS             114..1460
                     /locus_tag="PLP58_21840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ11239.1"
                     /translation="MHALPASGPVRAGKDAAVWVRPGLVEEYRVSTDGVRQDFIVMQR
                     PAGAGEELAVDLEVTGARAETAAYGVKLTVSATGRELAYSRLKVTDAAGKELTARLDV
                     VDAGRLRVIVRDAGAAYPLRIDPTFSDADWVALNPSISGANSTVLAFAVDGGGNLYAG
                     GEFTAIGTVAASRIAKWDGSAWSALGTGMNSDVSALAVSGGDLYAGGAFTTAGGTSAN
                     RIAKWDGSAWSALGTGMDNSVRALAVSGGELYAGGNFTAAGGTTVNRIAKWNGSAWSA
                     LGSGMNSTVRALAADASGHLFVGGSFLTAGTTFTPYIAQANISGLTPIESWRQVHFGT
                     TDNTGNAADLFDHDLDGLVNLLEYAFGLNPTLITPTLPGLTREGGYLTMTITKQPGVT
                     YEVQTAGDLATGQPDSFSAASTTVLINDATTLKVRDNILISSTARRFLQVLVTAAP"
     gene            1626..>3455
                     /locus_tag="PLP58_21845"
     CDS             1626..>3455
                     /locus_tag="PLP58_21845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009957951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042625 - ATPase-coupled ion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HRJ11240.1"
                     /translation="MNDRASEDRPWAEALADFLLQQKGVEAIRLNPAERTVQVATLGR
                     VDTALLQARLNEVLRALDAHLLAAREAGRPDLGPVQLTEEAGAVRLEKPSCPTAPRFW
                     KWRDFEWPEPEEMEARSREEWRAMAAQAAVCGTALVAGFVVEKVGAGPEWLARALFGL
                     ALVSGGWDAAKDAWENLRERRLDVHFLMLAVAAGAVAIGAWEEGALLLFLFSTSGALE
                     HYVLHRTHREINALTKAAPKLARVVLADGSTEERAVGLLRAGEVIQVRPAELFPVDAV
                     VTIGETAADESTLTGESVPVDKGVGAVVYGGTLNLWGLVRARVERPATQSALAKIISL
                     IQTAQHLRAPSQRFTDRFGTNYTLLTLGTVLGMFLVWWLALGIPPMENTAESKSAFYR
                     AMTLLVVMSPCALVLSIPSAILAAIAWGARRGILFRGGAAIEKLAEVEVVAMDKTGTL
                     TEGNLRVAAVESFPPGREEEVLRLAVTLDAHSNHPVARAITSHAREQGIEPGELLEYE
                     SIPGQGLRGRTAEGVVYAGRRELIGQGGFDAWLKDVPDAPLGFSEVWVLHEKAMGRIL
                     LKDEIRAGSKAVLEALAAEGVLTVMLTGDRRAAAAQVAQELGVA"
BASE COUNT          518 a         1024 c         1397 g          516 t
ORIGIN      
        1 tgaatatcaa ctgcttatca gtcgaatgga cgtagccccc cgcggggacg acagccggtt
       61 ccccggccgc gatttcagcc gccacggcgc taggagcacc aggcgccctg cctttgcacg
      121 cgctccccgc ctccggcccc gtccgcgcag gcaaagatgc cgccgtgtgg gtgcgtcccg
      181 gtctggtgga ggaataccgc gtcagcacgg atggcgtgcg ccaggacttc atcgtcatgc
      241 agcgcccggc gggggcgggg gaggagctgg ccgtggacct ggaggtcacc ggcgcgcggg
      301 cggagacggc ggcctacggc gtgaagctca ccgtcagcgc caccggccgc gagctggcct
      361 acagccgcct gaaggtcacc gatgcggcgg ggaaggaact caccgcccgc ctcgacgtgg
      421 tggatgccgg gcgcctgcgc gtgatcgtgc gggacgccgg cgcggcctac cccctgcgca
      481 ttgaccccac cttctccgat gcggactggg tggcgctcaa tcccagcatc tcaggggcca
      541 acagcaccgt tttggccttt gcggtggatg gcggcgggaa cctgtatgcg ggcggggaat
      601 tcaccgccat cggcacggtg gcggccagcc gcatcgcgaa atgggatggc agcgcctgga
      661 gcgccctggg aacgggcatg aacagcgacg tctctgcgct ggcggtgagc gggggcgatc
      721 tctacgccgg gggcgccttc accacggcgg gcgggacgag cgcgaaccgc atcgccaaat
      781 gggacggcag cgcctggagc gccttgggca cgggcatgga caacagcgtc cgtgcgctgg
      841 cggtaagcgg gggcgagctc tacgccgggg gaaacttcac cgcggcgggt gggactacgg
      901 tgaaccgcat cgcgaaatgg aacggcagcg cctggagcgc cctcggctcg ggcatgaaca
      961 gcaccgtccg ggcgctggcg gcggatgcca gcgggcatct cttcgtcggc ggcagttttc
     1021 tcaccgccgg caccacattc accccctata tcgcccaggc caacatcagc ggtctcaccc
     1081 ccatcgaatc ctggcggcag gtccacttcg gcaccaccga taacaccggc aacgcggcgg
     1141 atctctttga ccatgacctc gacgggctgg tgaacctgct ggagtatgcc ttcgggctga
     1201 atcccaccct catcaccccc accctccccg gcctgacgcg ggagggcggc tacctgacga
     1261 tgacgatcac caagcagccc ggcgtgacct atgaggtgca gacggcgggc gatctggcca
     1321 ccggccagcc ggattccttc agcgctgcga gcaccacagt gctcatcaat gatgcgacga
     1381 cgctgaaggt acgggacaac atcctcatca gcagcaccgc gcggcgtttt ctccaagtgc
     1441 tggtgaccgc ggcgccgtag ctcgcggttc gcgaagagcc gaagctgcaa ccggcggccc
     1501 gggtcgtcgc catcaatgac ctccagcttc tcgcggagat gtgcgaaagg cgggctgcat
     1561 ggctgccgcc gcaagacggc ggtttgggct ttcccggatg gagtttccgg cgtagccggt
     1621 gggtgatgaa tgaccgcgcc agcgaagacc gcccctgggc cgaggccctg gcggatttcc
     1681 ttttgcagca gaaaggggtg gaggccatcc ggctgaatcc ggcagagcgc acggtgcagg
     1741 tggccacgct ggggcgggtg gacacggcgc tgctccaggc gcggctcaat gaggtgctgc
     1801 gcgcgctgga cgcgcacctg ctggcggcgc gtgaggcggg ccgcccggac ctggggccgg
     1861 tgcaactgac ggaggaggcg ggagcggtgc gcctggagaa gccgagctgc cccacggcgc
     1921 cgcgcttctg gaagtggcgg gactttgagt ggccggagcc ggaggagatg gaggcgcgga
     1981 gccgggagga gtggcgggcg atggcggcgc aggcggcggt gtgcgggacg gcgctggtgg
     2041 cgggctttgt ggtggagaag gtcggcgcgg gaccggagtg gctggcgagg gcgcttttcg
     2101 gcctggcgct ggtgagcggg ggctgggacg cggcgaagga tgcctgggag aatttgcggg
     2161 agcggcggct ggatgtgcat ttcctgatgc tggcggtggc ggcgggggcg gtggccatcg
     2221 gcgcatggga ggagggggcg ctgctgctgt ttcttttctc gacctcgggg gcgctggagc
     2281 attatgtgct gcaccgcacg caccgggaga tcaacgcgct gacgaaggcg gcgccaaagc
     2341 tggcgcgcgt ggtgctggcg gacggctcca cggaggagcg cgcggtgggc ctgctgcgcg
     2401 cgggggaggt gatccaggtg cggccggcgg agcttttccc ggtggacgcg gtggtgacga
     2461 tcggggagac ggcggcggat gagtccacgc tgacggggga gagcgtgccg gtggacaagg
     2521 gcgtgggcgc cgtggtgtat ggggggactt tgaacctgtg gggcctggtg cgggctcggg
     2581 tggagcgccc ggccacgcag agcgcgctgg cgaagatcat cagcctgatc cagacggcgc
     2641 agcacttgag ggcgccgagc cagcgcttca cggaccgctt cggtacgaac tacacgctgc
     2701 tgacgctggg cacggtgctg ggcatgttcc tggtgtggtg gctggcgctg ggcatcccgc
     2761 cgatggagaa cacggcggag tcgaagtcgg ccttttaccg ggcgatgacg ctgctggtgg
     2821 tgatgagccc gtgcgcgctg gtgctgtcca tcccgtcggc catcctggcg gcgatcgcgt
     2881 ggggggcgcg gcggggcatc ctgttccggg gcggggcggc gatcgagaag ctggcggagg
     2941 tggaggtggt ggcgatggac aagacgggca cgctgacgga ggggaacctg cgggtggcgg
     3001 cggtggagag cttcccgccg ggcagggagg aggaggtttt gcgcctggcg gtgacgctgg
     3061 acgcgcactc gaaccatccg gtggcgcggg cgatcacgag ccatgcgcgg gagcagggca
     3121 tcgagccggg ggagctgctg gagtacgagt ccatccccgg ccaggggctg cgcgggcgca
     3181 cggcggaggg ggtggtgtat gcgggacggc gggaattgat cggccagggt ggctttgacg
     3241 cctggctgaa ggatgtgccg gacgcgccgc tgggcttctc ggaggtgtgg gtgctgcatg
     3301 agaaggcgat ggggcgcatt ttgctgaagg atgagatccg cgcgggcagc aaggcggtgc
     3361 tggaggcgct ggcggcggag ggggtgctga cggtgatgct gacgggggac cggcgcgcgg
     3421 cggcggcgca ggtggcgcag gagctgggcg tggcg
//
LOCUS       DAOMYJ010000371         3431 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Prosthecobacter sp. isolate AMR_MDS_5629
            NODE_9927_length_3431_cov_16.731931, whole genome shotgun sequence.
ACCESSION   DAOMYJ010000371 DAOMYJ010000000
VERSION     DAOMYJ010000371.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867850
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prosthecobacter sp. (wastewater metagenome)
  ORGANISM  Prosthecobacter sp.
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Verrucomicrobiaceae; Prosthecobacter.
REFERENCE   1  (bases 1 to 3431)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3431)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.30
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:34:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,742
            CDSs (total)                      :: 4,684
            Genes (coding)                    :: 4,676
            CDSs (with protein)               :: 4,676
            Genes (RNA)                       :: 58
            tRNAs                             :: 55
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3431
                     /organism="Prosthecobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9927_length_3431_cov_16.731931"
                     /isolate="AMR_MDS_5629"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1965333"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..248)
                     /locus_tag="PLP58_21850"
     CDS             complement(<1..248)
                     /locus_tag="PLP58_21850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ11241.1"
                     /translation="MNTRPLRTPALLLALALSCQTLAAQDNPPPRPPENPRRDAPPPE
                     ARERAEQAERETRERNERAERERRENEELLEHVGPDVTG"
     gene            complement(358..738)
                     /locus_tag="PLP58_21855"
     CDS             complement(358..738)
                     /locus_tag="PLP58_21855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018970948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="globin"
                     /protein_id="HRJ11242.1"
                     /translation="MDPLGTLYEMTGEPRLRGLVAAFYRRVRTDDLIAPLYPPDDWEG
                     AEKRLADFLIYRFGGPQTYIEERGHPRLRGRHMPFAIGTAQRDRWVELMDAALKETVM
                     PAQAAELMRPFFARVADFMRNREG"
     gene            858..1490
                     /gene="msrA"
                     /locus_tag="PLP58_21860"
     CDS             858..1490
                     /gene="msrA"
                     /locus_tag="PLP58_21860"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009965933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HRJ11243.1"
                     /translation="MKLPIVLTALVSLMSTAPMPAADAASPKTETAILGGGCFWCTEA
                     QYKLLKGVKKVVSGYANGHVDNPTYKQVCNGDTGHAEVVQIEFDPQVISYKDIVDFFW
                     LAHDPTTLNRQGNDVGTQYRSCILYADEEQKKIAEASMKEARKDWEDPIVTEIAPLKK
                     FYPAEDYHQDYFAINPNAGYCRVVVGPKVAKFREKLEAKGKIKEEASPAP"
     gene            1545..1877
                     /locus_tag="PLP58_21865"
     CDS             1545..1877
                     /locus_tag="PLP58_21865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ11244.1"
                     /translation="MPDPAETDSAFESYDAEVVDRVWGLARMIPGNDPEVWRKDEFGA
                     WVHRREYRNRHSEFGWEIADYGFQRRGCGLASLRPMQWQNHVDFLVAGRRQAVVSADG
                     LRNARRLL"
     gene            2046..3008
                     /locus_tag="PLP58_21870"
     CDS             2046..3008
                     /locus_tag="PLP58_21870"
                     /inference="COORDINATES: protein motif:HMM:NF013567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HRJ11245.1"
                     /translation="MAENLRIGLIGLDTSHCEQFTLRLNDPANPNHVGGARVVAAFPA
                     GSPDLPESADRLAGYTALLRDKFGVQIKSGIPEVCAEVDAVMILSVDGRPHLEQARQV
                     ILSGKPFFLDKPVAASLKEAVEIYQMADAAKVPMFSASAIRWYPGVIEVAAAGDAPAL
                     GALSYGPAPILPHHPDLFFYGIHPAEALFTVMGSGCREVRRATSAALSVVTGVWQGGR
                     LGTLHALHSQPMGSPHYKLVRFGQKGVTEQKNQGDYTPLLREIVKFFQTRQPPVSPRE
                     TLEIYAFMEAAEESKRLGGQAVSLREVLLKAGSPEKWLPVPAEP"
     gene            complement(3125..>3431)
                     /locus_tag="PLP58_21875"
     CDS             complement(3125..>3431)
                     /locus_tag="PLP58_21875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ11246.1"
                     /translation="GSHVARCRADVDDAADDRRRVTTAARHLIQRTDYPHPPDLSKTS
                     NGSSPSATRSRACKGVEWQVAAAQAVEAIARASRWPCRTSEKARTRARTLLDVPLEL"
BASE COUNT          572 a         1144 c         1136 g          579 t
ORIGIN      
        1 ccggtgacat ccggaccgac atgttccagg agttcctcgt tttcgcggcg ttcgcgctcg
       61 gcgcgttcgt tacgctcgcg ggtttcgcgt tcggcctgct cggcgcgttc gcgggcttcg
      121 ggtggcgggg cgtcgcgccg gggattttca ggcggacgcg gcggcgggtt gtcctgcgcg
      181 gcgagggtct ggcaggagag ggccagcgcc agcaggaggg cgggggtgcg gaggggtcgg
      241 gtgttcatgg gtgggttgtc aggttgcgga cacgggtccg ttcacgcgcc taacgacacc
      301 cggcgcgggc tttgtgtgag tcgggtgaaa aaagtgccgg agcgccgcgc cccagactca
      361 cccctcgcgg ttgcgcatga agtccgccac ccgggcaaag aatgggcgca taagctccgc
      421 cgcctgcgcc ggcatgacgg tctccttgag ggcggcgtcc atgagttcca cccagcggtc
      481 gcgctgcgcc gtgccgatgg cgaagggcat gtggcgtccg cgcagccgcg gatgcccgcg
      541 ctcctcgatg taggtctgtg gaccgccgaa acggtagatg agaaagtccg ccagtcgctt
      601 ttccgcgccc tcccagtcat ccggcgggta aaggggggcg atgaggtcgt cggttcgcac
      661 cctgcggtaa aacgcggcca ccagtccgcg caggcgcggc tctccggtca tttcataaag
      721 cgtgcccaaa ggatccatgc cgcgcatcag agcatcgaaa tgcggcttga aaacccattg
      781 cgcaaaaaag gaatcgcgaa agttttcccc cgctcgtaac gcatccctgg ctgcacacca
      841 gcccgcctta tcgcgccatg aaacttccca ttgtcctcac agccctcgtc tccctcatga
      901 gcaccgcccc catgcccgcc gccgatgccg ccagcccgaa gaccgagacc gccatcctgg
      961 gcggcggctg cttctggtgt accgaggccc agtacaagct gctcaaaggc gtgaaaaaag
     1021 tggtcagcgg ctacgccaac ggccatgtgg acaaccccac ttacaagcag gtctgcaatg
     1081 gcgacaccgg ccacgcggaa gtcgtccaga tcgagttcga tccgcaggtc atcagctaca
     1141 aggacatcgt ggacttcttc tggctggcgc atgatcccac cacgctcaac cgccagggca
     1201 acgacgtcgg cacccagtac cgctcctgta ttctttatgc ggacgaggag cagaaaaaaa
     1261 tcgccgaggc ctccatgaag gaggcgcgga aggactggga ggacccgatc gtcacggaga
     1321 tcgcgccgct gaaaaaattc tacccggcgg aggactacca ccaggactac ttcgccatca
     1381 accccaacgc cggctactgc cgcgtggtgg tggggccgaa ggtggccaag ttccgcgaaa
     1441 agctggaggc caagggcaaa atcaaggagg aggccagccc ggcgccgtga ggcggcgctc
     1501 tttccttttg agagccggcg cgtgacggcg tagtacaccc ggcaatgccc gaccccgccg
     1561 aaaccgacag cgcctttgaa agctatgacg ccgaagtggt ggaccgtgtc tgggggctgg
     1621 cgcggatgat tccggggaac gaccccgaag tctggcgcaa ggacgagttc ggcgcgtggg
     1681 tacaccggcg cgaataccgg aacaggcaca gcgagttcgg ctgggagatc gccgattacg
     1741 gattccagcg gcgcggctgc ggtcttgcga gcctgcggcc catgcagtgg cagaaccatg
     1801 tggacttcct cgtggccggc cgccgccagg cggtggtcag cgccgacggc ctgcgcaacg
     1861 cgcggcggct gctctgaccg cgctgccgcg cctctccgcg gctggagaaa atgcccgcgg
     1921 ggtgctctgc gcgcttgcct gcggatgaag tctgctgcct tgtgggcggc atgtttgcca
     1981 gacaacacag acccgcccgc gcgtccatcg cggccgcgct tggcttgtcg ctttcttttc
     2041 ccatcatggc agaaaatctc cgcatcggcc tcatcggcct ggacacttcg cactgcgagc
     2101 agttcaccct caggctgaat gatcccgcga atcccaatca tgtgggcggc gcgcgcgtcg
     2161 tggccgcttt tcccgccggc agccccgacc tgccggagag cgcggaccgt ctcgccggct
     2221 acaccgccct gctcagggac aaattcggcg ttcagatcaa gagcggcatc cccgaggtct
     2281 gcgcggaggt ggatgccgtg atgatcctga gcgtggacgg ccgcccgcat ctggagcagg
     2341 cgcgccaggt catcctctcc ggcaagccct tcttcctcga caaacccgtg gccgcctccc
     2401 tgaaggaggc ggtggagatt tatcaaatgg cggacgccgc caaggtgccc atgttcagcg
     2461 cctccgccat acgctggtat ccgggcgtca tcgaggtggc cgccgccggg gacgcgcccg
     2521 cgcttggcgc cctctcatac ggccccgcgc ccatcctccc gcatcatccc gacctcttct
     2581 tttacggcat ccatcccgcc gaggcgctct tcaccgtcat gggatccggc tgccgcgagg
     2641 tgcgccgggc caccagcgcc gccctctccg tggtcaccgg cgtctggcag ggcgggcgcc
     2701 tcggcacgct gcacgccctg cattcgcagc cgatgggctc cccgcattac aagctggtcc
     2761 gcttcgggca aaagggcgtc accgagcaga agaaccaggg ggactacacg ccgctgctgc
     2821 gcgagatcgt gaaattcttc cagacccgcc agccgcccgt ctcccccagg gagacgctgg
     2881 agatctacgc cttcatggag gccgccgagg agagcaagcg cctgggcggg caggccgtga
     2941 gcctgcgaga ggtcctgctc aaagccggct cccccgaaaa atggctgccg gttcccgccg
     3001 aaccgtgaat gcggcgggtg cgcccgcccg cattttgcca cgccccgcgc ccttcatgga
     3061 gtcggcggtt tgcggtttcc ctccgcgccg tccgagggac gctgaaatgt ctgctgactc
     3121 gccttcacaa ctccaagggc acatcgagaa gcgtgcgtgc tcgggtcctg gctttctcgg
     3181 atgttcggca aggccatctg ctggcgcggg cgattgcttc cacggcttgc gccgcagcta
     3241 cttgccactc cacgcctttg caggcgcggc tacgggtggc gctgggggag gagccatttg
     3301 aggtcttcga caagtcgggc gggtgggggt agtcggtgcg ctggatgagg tggcgggcgg
     3361 cggtggtgac gcggcggcgg tcgtcggcag catcatcgac gtcggcgcgg caccgtgcca
     3421 catgactgcc c
//
LOCUS       DAOMYJ010000372         3412 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Prosthecobacter sp. isolate AMR_MDS_5629
            NODE_10004_length_3412_cov_17.620494, whole genome shotgun
            sequence.
ACCESSION   DAOMYJ010000372 DAOMYJ010000000
VERSION     DAOMYJ010000372.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867850
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prosthecobacter sp. (wastewater metagenome)
  ORGANISM  Prosthecobacter sp.
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Verrucomicrobiaceae; Prosthecobacter.
REFERENCE   1  (bases 1 to 3412)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3412)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.30
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:34:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,742
            CDSs (total)                      :: 4,684
            Genes (coding)                    :: 4,676
            CDSs (with protein)               :: 4,676
            Genes (RNA)                       :: 58
            tRNAs                             :: 55
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3412
                     /organism="Prosthecobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10004_length_3412_cov_17.620494"
                     /isolate="AMR_MDS_5629"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1965333"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..514
                     /locus_tag="PLP58_21880"
     CDS             <1..514
                     /locus_tag="PLP58_21880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ11247.1"
                     /translation="LDAPASPQGFSRGLESSPQALSLGACRARREASFTNGEKVVLNI
                     PVLRRGNTPVDPSQVDINVFFFDRVNGREIAQTIADDPPTAWTSPPVDWSGIGEEPLE
                     VTYLMPNLTPGEVAAHGKRAFHGYVVRLYYQNKLQDVAAEPRDLLDFGSPAPRTDMGG
                     VNPLLPPAAP"
     gene            534..2471
                     /locus_tag="PLP58_21885"
     CDS             534..2471
                     /locus_tag="PLP58_21885"
                     /inference="COORDINATES: protein motif:HMM:NF015722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HRJ11248.1"
                     /translation="MTLLFLDSNAATRGPRTEALKAHPGWTVKSVPNVAEALAWLGSA
                     TRLDLLITEAVVDATSTGFELRDRALAKFPQARVLFTTRYDLSGFEDRIAGWTVLPDA
                     PFSPEKLVQKARAALGATPPPSIVPQPPHLDPGTMLGQYQVMERLTVEPESETYRAIQ
                     VTVQRPVALVLLRPDLLGQADAVADFKARERLKASISHPRIAPLYEASSAGGLIFYTR
                     EMPRGRPLEDILAAGEHYSERKIAEILFGVAEAMHHGVSRGHHHRRLMARDIFVDSEN
                     QASIVNLFRPGGSRPRVPEEEVRALLELVSPLAAEGKARGLISGLIEKNHDWAGLLED
                     LDDVREAMRERSIMRRIEAEESQVAADRNIPWWGWALVVGAVILVFVLGSELGKNGVG
                     ARNFVLEPEMVRIPAGRFIFQNGARRNLPDYWISRHEVTIGQYADFIAAIGGGTALDH
                     PSQPKTKTGHTPPKWAEILAAAKAGATYNGQPISLNTPVTQVDWWDAYAYARWRGQRL
                     PTEEEWEKAARGEDGRRLPWGAKPLKGAANLGEDYDANGKGGKVDGHVFWAPVDRQTQ
                     DISPFGIHDMAGNVSEWTASERAGETWPAHPDYPDLRVPVVRGGHFALKNTTDLLTTR
                     FFAESPDEATLTRGFRTAADK"
     gene            2548..3375
                     /locus_tag="PLP58_21890"
     CDS             2548..3375
                     /locus_tag="PLP58_21890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ11249.1"
                     /translation="MRLLIFLLLASAAHAQYAVNVSLPRTNFLALEAIPATVTITNRS
                     GAEVVLGGPGRASWLSFQMTDAKGYELGPIDVSGVDLAQIPPGGTIQRKITVTDAYAP
                     TDIGNYGLMARVLHTPTGQYFGSNRVRFAITDAKAMWEQEYGVPPGFRGEGAIRKYGI
                     VLFTDVDSTSLYLRLTDSKTGLRIQTYRLGPITMAHDPQITIDHENHLQVLFLAQPNL
                     WSHCVVAPDGVLKRRAYYRETSGNRPRLAQEASGAASVIGGVIYDPDAAPPVPRIAN"
BASE COUNT          602 a         1393 c          911 g          506 t
ORIGIN      
        1 gctcgacgca cccgccagtc cgcaaggctt ttcccgcggg ctggaaagct ccccccaggc
       61 gctctccctc ggcgcatgcc gggcccggcg ggaggccagc ttcaccaacg gcgagaaagt
      121 cgtcctcaac atccccgtcc tgcgccgtgg caacacgccg gtggacccct cccaggtgga
      181 catcaacgtc ttcttcttcg accgcgtgaa cggccgcgag atcgcccaga ccatcgccga
      241 cgatccgccc accgcgtgga cctcccctcc tgtggactgg tccggcattg gcgaggagcc
      301 gctcgaggtc acctacctca tgcccaacct cacccctggc gaggtggcag cgcacggcaa
      361 gcgcgccttc cacggctatg tcgtccgcct ttactaccag aacaaactcc aggatgtcgc
      421 cgccgagccg cgcgacctcc tcgacttcgg ctcacccgcg ccccggacag acatgggcgg
      481 agtgaatccg ttgctgcccc cggccgctcc gtgaatgcgc ggcctcccat gaaatgactc
      541 tccttttcct cgactccaac gccgccaccc gcggcccccg caccgaagcc ctcaaagccc
      601 accccggctg gactgtcaaa tccgtgccca acgtcgccga agcgctcgcc tggctcggct
      661 ccgccacccg gctcgacctc ctcatcaccg aggccgtcgt ggacgccacc agcaccggct
      721 tcgaactgcg ggaccgcgcg ctcgccaagt tcccccaggc gcgcgtgctt ttcaccaccc
      781 gctatgatct ttccggcttt gaagaccgga tcgccggctg gaccgtcctg cccgacgcgc
      841 ccttctcccc tgagaagctc gtgcagaaag cccgcgccgc ccttggcgcc acgccgccgc
      901 cctccatcgt ccctcagcca ccgcatctcg accccggcac catgctcggc cagtatcaag
      961 tcatggagcg cctcactgtg gagcccgagt ccgagaccta ccgcgccatc caggtcaccg
     1021 tgcagcggcc cgtggcgctc gtgctcctgc gccccgacct gctcggccag gcggacgccg
     1081 tcgctgactt caaggcgcgc gagcggctca aagcctccat ctcccatccg cgcatcgccc
     1141 cgctctatga ggcgtcctca gccggcggcc tcatctttta cacccgcgag atgccgcgcg
     1201 gacgcccgct ggaggacatc ctcgccgccg gggagcacta ctccgagcgc aagatcgccg
     1261 aaatcctctt cggagtcgcc gaggccatgc accacggcgt cagccgcggc caccaccacc
     1321 gccgcctcat ggctcgggac atcttcgtgg actccgagaa ccaggccagc atcgtcaacc
     1381 tcttccgccc cggcggcagc cgcccgcgcg tgccggagga ggaagtgcgc gccctgctcg
     1441 aactcgtcag ccccctcgcc gccgaaggca aggcgcgcgg cctcatctcc ggcctcatcg
     1501 agaaaaacca cgactgggcc ggcctgctcg aggatctcga cgacgtgcgc gaggccatgc
     1561 gcgagcgcag catcatgcgc cgcatcgagg ccgaggaaag ccaggtcgcc gccgaccgca
     1621 acatcccctg gtggggctgg gcgctggtcg tcggcgcggt catactcgtc tttgtcctcg
     1681 gctccgagct gggcaaaaac ggcgtcggcg ccaggaactt cgtcctggag ccggagatgg
     1741 tgcgcatccc cgccgggcgc ttcatttttc aaaacggcgc gcggcggaat ctgcccgact
     1801 actggatcag caggcacgag gtcaccatcg gccagtatgc cgacttcatc gccgccatcg
     1861 gcggcggcac cgccctggac cacccctccc agcccaagac caagaccggc cacaccccgc
     1921 cgaagtgggc ggaaatcctc gccgccgcca aagccggcgc cacctacaac ggccagccca
     1981 tcagcctgaa cacccccgtc acccaggtgg actggtggga cgcctacgcc tacgcgcgct
     2041 ggcgcggcca gcgcctgccc accgaggagg agtgggaaaa agccgcgcgc ggcgaggacg
     2101 gccgccgcct cccctgggga gccaaacccc tcaaaggcgc cgccaatctc ggcgaggact
     2161 acgacgccaa cggcaaaggc ggcaaagtgg acggccacgt cttctgggcg cccgtggacc
     2221 gccaaaccca ggacatcagc cccttcggca tccacgacat ggccggcaac gtctccgaat
     2281 ggaccgcctc cgagcgcgcc ggagaaacct ggcccgccca ccccgactac cccgacctgc
     2341 gcgtccccgt cgtgcgcggc ggccattttg ccctgaaaaa caccaccgac ctgctcacca
     2401 cccgcttctt tgccgaatct cccgacgaag ccaccctcac ccgcggcttc cgcaccgccg
     2461 cagacaaatg attcaaaatc gaatttcgat tttcgatttt cgaaatcaaa ctctccccaa
     2521 tccccacccc cccaatccca actcctcatg cgccttctca tcttcctcct cctcgccagc
     2581 gccgcgcacg cccagtatgc ggtgaacgtc tccctgccca ggaccaactt cctcgccctt
     2641 gaggccatcc ccgccactgt caccatcacc aaccgcagcg gagccgaagt cgtcctcggc
     2701 ggtcctggac gcgcctcctg gctcagcttt caaatgaccg acgccaaagg ctacgagctc
     2761 ggccccattg acgtctccgg tgtggacctc gcccagatcc cgcccggcgg caccatccag
     2821 cgcaaaatca ccgtcaccga cgcctacgcc cccaccgaca tcggcaacta cggcctcatg
     2881 gcccgcgtcc tgcacacccc caccggccag tacttcggca gcaaccgcgt ccgcttcgcc
     2941 atcaccgacg ccaaagccat gtgggagcag gaatacggcg tgccgcccgg tttccgcggc
     3001 gagggcgcca tccgcaaata cggcatcgtc ctcttcaccg acgtggacag cacctccctc
     3061 tacctgcgcc tcaccgactc caaaacaggc ctgcgcatcc agacctaccg cctcggcccc
     3121 atcaccatgg cccacgaccc gcagatcacc atagaccatg aaaaccacct ccaggtcctc
     3181 ttcctcgccc agcccaacct ctggtcgcac tgcgtcgtcg cgccggacgg agtcctgaag
     3241 cgccgcgcct actaccgcga gaccagcggc aaccgccccc ggctcgccca ggaggcctcc
     3301 ggcgccgcct ccgtcatcgg cggcgtgatt tacgatcccg acgccgcgcc ccccgtgccg
     3361 cgtattgcaa actgatgatc tgtacttcca tacagtgaac cataattatt at
//
LOCUS       DAOMYJ010000373         3388 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Prosthecobacter sp. isolate AMR_MDS_5629
            NODE_10125_length_3388_cov_17.255326, whole genome shotgun
            sequence.
ACCESSION   DAOMYJ010000373 DAOMYJ010000000
VERSION     DAOMYJ010000373.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867850
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prosthecobacter sp. (wastewater metagenome)
  ORGANISM  Prosthecobacter sp.
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Verrucomicrobiaceae; Prosthecobacter.
REFERENCE   1  (bases 1 to 3388)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3388)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.30
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:34:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,742
            CDSs (total)                      :: 4,684
            Genes (coding)                    :: 4,676
            CDSs (with protein)               :: 4,676
            Genes (RNA)                       :: 58
            tRNAs                             :: 55
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3388
                     /organism="Prosthecobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10125_length_3388_cov_17.255326"
                     /isolate="AMR_MDS_5629"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1965333"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..847)
                     /locus_tag="PLP58_21895"
     CDS             complement(<1..847)
                     /locus_tag="PLP58_21895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009958870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1501 domain-containing protein"
                     /protein_id="HRJ11250.1"
                     /translation="MSSTSSQPPRACTENSKLEIRRPRPDIPTSNFDFRSSNFLPRRW
                     FLRDCGVGLAGIAAHSLLSREAGGAVPRRAGHHEPRAKRVIYMFQAGAPSHLELFDPK
                     PELAKRDGQLPPADLLKNYRAAFIQPDSALLAPKYKFNRHGQCGMELSEILPHTGSIA
                     DEICLVRSMQTDAVNHAPGQILMNTGSEQFGRPSFGAWTLYGLGSDSENLPGYVVLTS
                     AKGTSGGASNYGSGFLPTLYGGVPLRSTGDAVLYLSNPKGVDAAAQRASLDTLHELNR
                     QSFGAF"
     gene            complement(867..1337)
                     /locus_tag="PLP58_21900"
     CDS             complement(867..1337)
                     /locus_tag="PLP58_21900"
                     /inference="COORDINATES: protein motif:HMM:TIGR02436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four helix bundle protein"
                     /protein_id="HRJ11251.1"
                     /translation="MMLSEFTETEEDFPSSGELDFWGVHEAAITLPASADSAMLREEA
                     RPYDLGERTAVFGQKVIRLAKTVPFNPVNNPILTQLVKAATSPGANYCEANDSISAKD
                     FKHRISICRKEAKETRYWLRMLATSEPKITDEARALWREATELNLIFPSIWRKA"
     gene            complement(1396..>3388)
                     /locus_tag="PLP58_21905"
     CDS             complement(1396..>3388)
                     /locus_tag="PLP58_21905"
                     /inference="COORDINATES: protein motif:HMM:NF019223.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF1553 domain-containing protein"
                     /protein_id="HRJ11252.1"
                     /translation="TSIRDRLKKQTPDWQEKLAAWEKSVAAQQAEWQPLPPKKLMTVS
                     GLNHPTRLEDESILTLGHPSSSSDHLIFAEPALDGVTGLRLEALTHRDLPHTGPGHSR
                     HGIWAITELECHTQKPGEKDWQKVEMKNATADFSEPDHRIEPEWQTKADEKSERRVGP
                     VAFLIDGDNQTAWRADRGPGRRNAPSVAVVQFATPLKAPAGTRMRLTVRMNHSGDGNG
                     RGQRAIAMLGRMRFSTTRSPTPKTPAVDHAAQLAMQVPAEKRGADEHDALLTAWSRTL
                     PEAKKLNDEAEALWKNWPAEPKTSVLHLAEREPEMARSTHLLERGVWNQPKHDVRPHV
                     PAALHPLPAGDTADRLTFARWLVDERSPLTARVAVNRVWQTLFGTGLVETSEDFGTRA
                     PTPEHHALLDWLAADFMRNGWSQKHLLRTIVTSSVYQQRSTLTPELREKDPNNRLLAR
                     GPRFRADAETVRDMALAISGLLHEQVGGPSIYPPVPQSVLDYNYNPPDYWHAPTDAQR
                     YRRSLYHFRKRSMPDPVLQAFDAPNGDFSCARRTRSNTPLAALTSLNETLFVEAAQAL
                     AQRILREGGGSDESRIRRAYLLCTSRAPTAAETGAVLRLLEQNRARLRAGELKAGEIA
                     FSPLTKPEALPPDATPNDIASWTLVARVLLNLDETLSKS"
BASE COUNT          540 a          935 c         1251 g          662 t
ORIGIN      
        1 cgaaggcgcc gaaagactgg cggttcagct cgtgcagcgt gtcgagcgag gcgcgctggg
       61 cggcggcgtc cacgcctttc ggattggaca ggtagagcac ggcatcgccc gtggagcgca
      121 ggggcacgcc gccgtagagg gtggggagga aaccggagcc gtagttgctg gcgccgccgc
      181 tggtgccctt ggcgctggtg agcacgacgt aaccaggcag gttttcactg tcgctgccca
      241 ggccgtaaag cgtccacgcg ccgaagctcg ggcggccgaa ctgctcggag ccggtgttca
      301 tgaggatctg gccgggcgcg tgattgacgg catccgtctg catggagcgc accaggcaga
      361 tctcgtcggc aatggagccg gtgtgcggca ggatctcgct caactccatg ccgcactggc
      421 cgtgacggtt gaatttatac ttgggcgcga gcagggcgga gtccggctgg atgaaggcgg
      481 cgcggtagtt tttcagcaaa tcggcgggtg ggagctgccc gtcccgtttt gccagctccg
      541 gcttcggatc gaaaagctcc agatgactcg gcgcacccgc ctggaacatg tagatgaccc
      601 gcttcgccct tggctcgtga tgccccgcgc gcctcggcac ggcacccccc gcctcccgcg
      661 agagcaaaga gtgcgcggca atgcccgcca gtccgactcc gcagtcgcgc agaaaccagc
      721 ggcgggggag gaaattcgaa gatcgaaaat cgaaattcga agttggtata tcgggcctcg
      781 gtcttcgaat ttcgagcttc gaattttccg tgcaggctcg tgggggctga gaagaggtgg
      841 aggacatgtg gatcagtttt gaggaattag gcttttctcc agatgctggg gaagatcagg
      901 ttgagttccg tggcctcgcg ccagagggcg cgggcttcat ccgtgatttt gggttctgaa
      961 gtggcaagca tgcgcagcca gtagcgagtt tctttggcct ccttgcggca gatggagatg
     1021 cggtgcttga agtctttggc ggaaatcgag tcattggcct cgcagtagtt ggctccaggg
     1081 ctggtcgccg ctttgaccaa ctgggtaagg atcggattgt tcacgggatt gaagggaaca
     1141 gtcttcgcga gccggatcac cttctgtccg aataccgccg tgcgctcgcc gagatcataa
     1201 gggcgggctt cttccctcaa catagcagag tcagccgacg ctggaagggt gatggcggct
     1261 tcatggactc cccaaaagtc caactccccc gaagaaggaa agtcttcttc ggtttcggtg
     1321 aattcagaaa gcatcatggt ttcagagggt tcttcgaaat tcgaaatgtg agcttcgagc
     1381 ttcgaatttc gaatttcaag acttgctcaa ggtctcgtcc aaattcagca gcacccgcgc
     1441 aacgagagtc caggaggcga tgtcgttggg ggtggcgtcg ggcgggaggg cctccggttt
     1501 ggtgagcggg gaaaaggcga tctctccggc tttcagttcg cccgcgcgga ggcgggcgcg
     1561 gttttgctcc aggagacgga gcacggcgcc ggtctcggcg gcggtggggg cgcggctggt
     1621 gcagagaaga taggctcggc ggatgcgcga ctcgtcactg ccgccgccct cgcgcaggat
     1681 gcgctgggcc agggcctggg cggcttcgac aaagagcgtt tcattgaggc tggtgagggc
     1741 ggcgaggggt gtgttcgagc gggtgcggcg cgcgcaggaa aagtcgccgt tcggcgcgtc
     1801 gaaagcctgc agcacggggt cgggcatgga gcgtttgcgg aagtggtaga ggctgcggcg
     1861 gtagcgctgg gcatcggtgg gcgcgtgcca gtagtcggga gggttgtaat tgtaatcgag
     1921 cacgctctgg ggcacgggcg ggtaaatgct ggggccgccg acctgctcgt gcagcaggcc
     1981 ggagatggcg agggccatgt cgcgcacggt ctcggcatcg gcacggaagc gcgggccgcg
     2041 ggcgagcagg cggttgttcg ggtccttctc ccgcagttcg ggcgtgaggg tggagcgctg
     2101 ctgatagacg ctgctggtga cgatggtgcg caggaggtgt ttctggctcc agccgttccg
     2161 catgaaatcc gcggccagcc agtcgagaag ggcgtggtgc tccggcgtgg gcgcgcgcgt
     2221 gccgaagtcc tcggaggtct ccacgaggcc cgtgccgaac aaagtctgcc aaacgcggtt
     2281 caccgccacg cgtgcggtga gaggcgagcg ctcgtccacg agccagcggg caaaggtgag
     2341 gcggtcggcc gtgtcaccgg cgggcagggg atgcagcgcg gcgggaacat gcggccgcac
     2401 gtcgtgcttc ggctggttcc agacgccgcg ctcgagcagg tgcgtggatc gggccatctc
     2461 cggttcgcgc tcggcgaggt ggagcacgga ggtcttcggc tcggcgggcc agtttttcca
     2521 caaggcctcg gcctcgtcgt tgagcttttt ggcttcgggt aaagtgcggc tccatgcggt
     2581 gagaagggcg tcatgctcgt cagcgccgcg tttttccgca ggcacctgca tggcgagctg
     2641 cgcggcgtgg tccacggcgg gcgtcttcgg cgtcgggctg cgggtggtgg agaagcgcat
     2701 gcgtccgagc atggcgatgg cgcgctggcc gcggccgttg ccgtcgccgc tgtgattcat
     2761 gcgcacggtg aggcgcatcc gggtgccggc gggggccttc aaaggcgtgg caaattgcac
     2821 gacggccacg gagggggcgt tgcgtcgtcc gggacctcga tccgcgcgcc aggcggtctg
     2881 gttgtcgcca tctatgagga aggccacggg gccgacacgg cgctcgctct tttcatccgc
     2941 cttggtctgc cattcgggtt cgatgcggtg gtcgggctcg gagaaatcgg cggtggcgtt
     3001 tttcatctcc accttttgcc agtctttctc tcccggcttc tgggtgtggc actccagctc
     3061 ggtaatggcc cagatgccat gccggctgtg cccagggccg gtgtgaggca gatcgcggtg
     3121 ggtgagcgcc tcaaggcgca ggccggtgac gccgtcaagc gcgggctcgg caaagatgag
     3181 atgatcgctg ctggatgagg gatgtccgag ggtgagaatg gattcatcct ccaagcgcgt
     3241 ggggtggttc aacccgctga cggtcatgag tttttttggc ggcaaaggct gccattcggc
     3301 ctgctgtgcg gcgacacttt tctcccaggc ggccagcttt tcctgccagt cgggggtttg
     3361 ctttttcagc cggtcccgga tgctggtg
//
LOCUS       DAOMYJ010000374         3384 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Prosthecobacter sp. isolate AMR_MDS_5629
            NODE_10146_length_3384_cov_17.198258, whole genome shotgun
            sequence.
ACCESSION   DAOMYJ010000374 DAOMYJ010000000
VERSION     DAOMYJ010000374.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867850
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prosthecobacter sp. (wastewater metagenome)
  ORGANISM  Prosthecobacter sp.
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Verrucomicrobiaceae; Prosthecobacter.
REFERENCE   1  (bases 1 to 3384)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3384)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.30
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:34:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,742
            CDSs (total)                      :: 4,684
            Genes (coding)                    :: 4,676
            CDSs (with protein)               :: 4,676
            Genes (RNA)                       :: 58
            tRNAs                             :: 55
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3384
                     /organism="Prosthecobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10146_length_3384_cov_17.198258"
                     /isolate="AMR_MDS_5629"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1965333"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..341
                     /locus_tag="PLP58_21910"
     CDS             <1..341
                     /locus_tag="PLP58_21910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007415199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HRJ11253.1"
                     /translation="AARSGAAIKGAVSFHGSLDSPAPADGANIKCRVLALHGADDPFV
                     SAADLAAFEKEMKDHRVDCKLVQYPGAVHAFTQKMAGDDPSKGAAYQEAADKASWEEM
                     KGFFRAIFGP"
     gene            338..1243
                     /locus_tag="PLP58_21915"
     CDS             338..1243
                     /locus_tag="PLP58_21915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ11254.1"
                     /translation="MTHPPPMTRSGTAILLAALLLAAHAPAQEKSAEPLGTDFLRFVE
                     DENEASLQTVLATYERADGVRAALAGAVHIADKAYFDDLNERFKKYDAVLYEMVGGPY
                     PKKKAARAQEAADGHHEEGDGGRLQWVGQMQEMMRKMLELEGQMACVNYGAPNFIHAD
                     MTTEGFFQSQEEKQETFLTLWLKSIQAQNSRVDARPRTGMAELLRILMSGDSATEMKR
                     VIGREFDSVEEILAGVEAGGGTTLIGERNRHALEVLDREIAAGKKNLAVFYGAAHLPD
                     MEKRLLERGFKLIRVEHLKAWLLKP"
     gene            1315..1399
                     /locus_tag="PLP58_21920"
     tRNA            1315..1399
                     /locus_tag="PLP58_21920"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1349..1351,aa:Leu,seq:aag)
     gene            1731..2786
                     /gene="glpX"
                     /locus_tag="PLP58_21925"
     CDS             1731..2786
                     /gene="glpX"
                     /locus_tag="PLP58_21925"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009962966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042132 - fructose 1,6-bisphosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphatase"
                     /protein_id="HRJ11255.1"
                     /translation="MNPAELPPLRSTLDLERVLEFEFVRATENAALQSFHWLGRGQKE
                     LADAAACAAIYGVFDLLDIRGEVVIGEGIKDNAPGIFIGEHLGTWRPGAPRFDIALDP
                     IDGTTNIAKGTPNSISVIAAAQKPEGAPCAMRSIPSFYCHKLAYGPAVKRALQGAGDR
                     PLLDMPLHEVIRFVADCLGKNIRDVVVVTMDRPRHHGIIEEVRSCGAALRMITDGDIA
                     AAVAPSLPDGGVDMYVGMGGSPEAVLAAAALKCLGGDMDARMWFHTPEHRAEVAAQCS
                     GEEMTRVFRSGDLIQGESALFCATGISDSPLVPGVKITGHRAETHSILMRARSGTIRH
                     IRASHDLDRKIVPIREA"
     gene            complement(2807..3166)
                     /locus_tag="PLP58_21930"
     CDS             complement(2807..3166)
                     /locus_tag="PLP58_21930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ11256.1"
                     /translation="MPKSVSHFPKCGADFPKCGADFPKCGADFPKCGAHLPKCGADFP
                     KCDADFPKCDAVFPKCDAVFPKCGWHLSECDAVFPKCGADFPKCGWHLPKCGADFPKC
                     DADFPKCGWHLPRHRAV"
BASE COUNT          636 a         1302 c          988 g          458 t
ORIGIN      
        1 aggccgcgcg ctccggcgcc gccatcaagg gcgccgtcag cttccacggc agcctggact
       61 cccctgcccc cgccgacggc gccaatatca aatgccgcgt cctcgccctg cacggcgccg
      121 acgacccctt cgtctccgcc gccgacctcg ccgccttcga aaaggaaatg aaggaccacc
      181 gcgtggactg caagctcgtg caataccccg gcgccgttca tgccttcacc cagaaaatgg
      241 ccggggacga cccctccaaa ggcgccgcct accaggaggc ggcggacaag gcatcgtggg
      301 aggagatgaa aggcttcttc cgcgccatct tcggcccatg acacacccac cgcccatgac
      361 acgctcaggc acagccatcc tcctcgcagc cctcctcctc gccgcccacg cgcccgcgca
      421 ggaaaaaagc gccgaaccac tgggcacgga tttcctccgc ttcgtcgagg acgaaaacga
      481 ggcctccctg caaaccgtgc tcgccaccta cgagcgcgcc gacggcgtgc gcgccgccct
      541 cgcaggcgcc gtccacatcg ccgacaaagc ctacttcgac gacctcaacg agcgcttcaa
      601 aaaatacgac gccgtgctct acgagatggt cggcggcccc taccccaaaa agaaagccgc
      661 acgcgcgcag gaggcggcgg acgggcatca cgaagaaggc gatggcggcc gcctccagtg
      721 ggtggggcag atgcaggaaa tgatgcgcaa gatgctggaa ctcgaaggcc agatggcctg
      781 cgtcaactac ggcgcgccca acttcatcca cgccgacatg accaccgagg gcttcttcca
      841 gtcccaggag gaaaagcagg agaccttcct caccctctgg ctcaaatcca tccaggcgca
      901 gaactcccgg gtggacgccc gcccccggac cggcatggcc gagctgctcc gcatcctcat
      961 gagcggcgac agcgccaccg aaatgaagcg cgtcatcgga cgcgagttcg acagcgtcga
     1021 ggaaatcctc gccggagtcg aagccggcgg cggcaccacc ctgatcggcg agcgcaaccg
     1081 ccacgccctg gaggtcctcg accgcgagat cgccgccgga aagaaaaacc tggccgtctt
     1141 ttacggcgcc gcgcacctgc ccgacatgga aaagcgcctg ctggagagag gcttcaaact
     1201 catccgcgtc gagcacctca aagcatggct cctcaagcct tgaaaaaagt gcaaactagc
     1261 ggttgcaagc cgcccccgga agctatactt cgcgcccctg cgttcagcag ccctgctcgg
     1321 gtggcggaat tggtatacgc gcaagactaa ggatcttgtg ccgcaaggct taagggttcg
     1381 agtcccttcc cgagcaccac ttcattttcg aggtgtcagc ggcacgtttt cccgccgcga
     1441 acaggcggaa aatcgtcccc gccccgcaat gcctccgcct cggaaacgcg cccgcgaaga
     1501 cgcctcatgc ccgcccggca ttccaccgcg cccagcgccg aagtccggat cgagagttcg
     1561 gtaatcctcc ccccggcgac gcgcgtcaca aacagccggt tgcgcgtgat cgagatgaca
     1621 caatgagccg cgaaaaagca gccggggaaa gtcgccgcca tcccgccgcc ccgccatccc
     1681 gccactttcc gctttcctcc gcccgcaagc cccgctttca tcccgcaccc atgaaccccg
     1741 ccgaactccc cccgctgcgc agcaccctcg acctggaacg cgtgcttgag ttcgagttcg
     1801 tccgcgccac cgaaaacgcc gccctccaga gcttccactg gctcggccgc ggccagaagg
     1861 agctggccga cgccgccgcc tgcgccgcca tctacggcgt cttcgacctg ctcgacatcc
     1921 gcggcgaggt cgtcatcggc gagggcatca aggacaacgc ccccggcatc ttcatcggcg
     1981 agcacctcgg cacctggcgg cccggcgcac cccgcttcga catcgccctc gaccccatag
     2041 acggcaccac caacatcgcc aaaggcaccc ccaacagcat ctccgtcatc gccgccgccc
     2101 agaagcccga gggcgcgccc tgcgccatgc gcagcatccc cagcttctac tgccacaagc
     2161 tcgcctacgg ccccgccgtc aagcgcgccc tccagggcgc cggagaccgc cccctgctcg
     2221 acatgcccct ccacgaggtc atccgcttcg tcgccgactg cctcggcaaa aacatccgcg
     2281 atgtcgtcgt cgtcaccatg gaccgccccc gtcaccacgg catcatcgag gaggtccgct
     2341 cctgcggcgc cgccctgcgc atgatcaccg atggcgacat cgccgccgcc gtcgccccct
     2401 ccctgcccga cggcggcgtg gacatgtacg tcggcatggg cggcagcccc gaggccgtgc
     2461 tcgccgccgc cgccctgaag tgcctcggcg gcgacatgga cgcgcgcatg tggttccaca
     2521 cccccgagca ccgcgccgaa gtcgccgccc agtgctccgg ggaggaaatg acccgcgtct
     2581 tccgcagcgg cgacctcatc cagggcgaga gcgcgctctt ctgcgccacc ggcatcagcg
     2641 acagcccgct ggtccccggc gtcaaaatca ccggccaccg cgccgagacc cacagcatcc
     2701 tcatgcgcgc ccgcagcggc accatccgcc acatccgcgc cagccacgac ctcgaccgca
     2761 agatcgtccc catccgcgaa gcttgagcac cccgcctgcc gccgcctcag actgcccggt
     2821 ggcggggcaa atgccagccg cacttgggga agtccgcgtc gcacttggga aagtccgcgc
     2881 cgcacttggg caaatgccag ccgcacttgg gaaagtccgc gccgcacttg gggaagaccg
     2941 cgtcgcactc ggacaaatgc cagccgcact tggggaagac cgcgtcgcac ttggggaaga
     3001 ccgcgtcgca cttggggaag tccgcgtcgc acttgggaaa gtccgccccg cacttgggca
     3061 aatgcgcgcc gcacttgggg aagtccgcgc cgcacttggg gaagtccgcg ccgcacttgg
     3121 ggaaatccgc gccgcacttg gggaagtgcg aaacggactt gggcaaatgc cagccgcgct
     3181 cggcctaatg cgcgcgggac tctgggaagt gccgtccgca ttcgggcaat tacgggccgg
     3241 attttccgcg cgccgggcag tattttgggg aatgcgcact ggcgcgccgc acttcagcac
     3301 cggcatcccc gcagccgcac aggcgcacgc gaggccgcca ggggtggctg cggcgctcaa
     3361 aggcggccac ggggctgcgc ggtc
//
LOCUS       DAOMYJ010000375         3356 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Prosthecobacter sp. isolate AMR_MDS_5629
            NODE_10284_length_3356_cov_21.577704, whole genome shotgun
            sequence.
ACCESSION   DAOMYJ010000375 DAOMYJ010000000
VERSION     DAOMYJ010000375.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867850
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prosthecobacter sp. (wastewater metagenome)
  ORGANISM  Prosthecobacter sp.
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Verrucomicrobiaceae; Prosthecobacter.
REFERENCE   1  (bases 1 to 3356)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3356)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.30
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:34:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,742
            CDSs (total)                      :: 4,684
            Genes (coding)                    :: 4,676
            CDSs (with protein)               :: 4,676
            Genes (RNA)                       :: 58
            tRNAs                             :: 55
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3356
                     /organism="Prosthecobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10284_length_3356_cov_21.577704"
                     /isolate="AMR_MDS_5629"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1965333"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..451
                     /locus_tag="PLP58_21935"
     CDS             <1..451
                     /locus_tag="PLP58_21935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ11257.1"
                     /translation="PSHRSHRAKRLSSIAAFGFKKQIGFLAARHQTSAMQVTLDLPDS
                     LAKRLPAEPGQLASILERGLRGMWAATAGAGKEVIEFLASGPSPSAIVTFKPSAQFAE
                     RSRDLLEKNTAGRLAPEEAAELDELAQLDSLVSILKAEARKRLQQSA"
     gene            448..864
                     /locus_tag="PLP58_21940"
     CDS             448..864
                     /locus_tag="PLP58_21940"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013964.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease signature motif containing
                     protein"
                     /protein_id="HRJ11258.1"
                     /translation="MSTTRISAALRRLVAQRAAGCCEYCRIHENEVLFAHEVDHVVAE
                     QHGGQTVEENLCLACFQCNRLKGPNLAGIDTLTGAVEVLFHPRLDDWCSHFRFIGAEI
                     EARTPRGRATVNMLRMNAPDRVRLRGMLIEAGRRFA"
     gene            1067..>3356
                     /locus_tag="PLP58_21945"
     CDS             1067..>3356
                     /locus_tag="PLP58_21945"
                     /inference="COORDINATES: protein motif:HMM:TIGR02604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HRJ11259.1"
                     /translation="MPRKQITYGVPCLVFDLPLLTLLSAAILCAQEPYKKPVSVGEPP
                     EDHSPAAELATFQVLEGFEVNLFADESDGIANALVIRWDEKGRLWCLQTSAYPQPAPD
                     EPADDKVIILEDTDGDGRADKSTVWASGLRMPMGMELAGPGQLYVGEGEKLLLLTDTD
                     GDDRADRREVIFSGFGTGDTHQNLNSFVWAPEGCLVMHQGLHCYSRVVTPHGVAQLYG
                     AGFWRYWPRSQRLEAYPTGMPLNPWGTAFTREGQPIMVAGAAGMFWARPMEIAVPEVP
                     PEQAGGTGTTAGLPYFLLTRHMLPHSGQIIKTSGLRKFCGVDIAGGPAWPGGMHGEVV
                     TGGFFENAVYRHQLIEDKDAPSGFEAVEQTPLITSSSVAFRPVDVRFGPDGALYIADW
                     YNPIIGHYQASFRHPNRDKQHGRIWRVAAKGRELSRPNLATMAWVRESSKFDQQKTAL
                     LLSQTEIKGDLWLAYQAERILRFLPDNHAIRAASWELGIDDSMEIVIPPDAPEQLRRE
                     LEEVIGNHKAWVHQQHHRILRAMVSFSESVHLEYLQTCLGDGRSTIRAWATHSIGDCA
                     HQLPDVLALLEPRIADPHPRVRLEAIVACAKLKSADALRLALKALDAPMDTFLDRALW
                     LAVHATAPQWKQPGTLAPFLAALPPEHLSYVLEKDGSRELIETAREMLASGADTLPGP
                     TRLALHRILIRKGGVEDQMRALQAGMEDIAVLTELKLTLGAQAKAPAPEGALPLLLEL
                     LASDSEDRRAAACELAAVWRLMP"
BASE COUNT          631 a         1061 c         1079 g          585 t
ORIGIN      
        1 cccatcgcac cgttctcacc gcgcaaaacg actttcttca atcgcggcgt tcgggttcaa
       61 gaaacagatt ggatttttgg cggcgcgaca tcaaactagc gccatgcaag tcacactaga
      121 tcttccagat tcgttggcaa agcggctgcc ggccgagccg gggcagcttg cctcgattct
      181 ggaacgcgga ctgcgcggca tgtgggctgc cactgcgggt gctggcaaag aagtgatcga
      241 gtttctggcc agcgggcctt ctccgtcggc catcgtgact ttcaagccgt cggcacagtt
      301 cgcggagcgc agccgtgatc ttttggaaaa gaacaccgca ggccgcctcg cgccggaaga
      361 ggccgccgag cttgatgaac tggcccagct cgactcgctg gtttccatct tgaaagccga
      421 ggcacggaaa agactccagc agtcggcatg agcacgacac gcatcagcgc ggccctgcgc
      481 aggctggtgg cgcagcgggc agccggatgc tgcgaatatt gccgcattca tgagaacgaa
      541 gtgctgttcg cccacgaggt ggatcatgtc gtggcagagc aacatggtgg tcagacggtc
      601 gaggaaaacc tctgtctggc ctgttttcag tgcaaccggc tcaaaggccc caatctcgcg
      661 ggcattgaca cactgaccgg cgcggtcgaa gtgcttttcc atccaaggct ggatgactgg
      721 tgcagccact tccgctttat cggagccgag atagaggccc gcacaccacg aggacgcgcc
      781 acagtgaaca tgctgcgcat gaacgctccc gatcgcgtgc ggctccgcgg catgctgatc
      841 gaagccggac gtcggtttgc gtgagaggct ggcattcaag ggattcatgc tttttgccct
      901 gcgctgaccc atccagcacg actttgccgc cgaatggcga gttccaggag cctgcgaagg
      961 tggctgattc aatgccggcc agggaagtca accaagcccg acaaattctc cggcggtcaa
     1021 aaaaagcctt cattgatccc gaatcccaag ggaagatgcg agcacgatgc caagaaaaca
     1081 aatcacctat ggggtgccat gccttgtctt tgacctgcca ctcctgacgc ttctgtcggc
     1141 tgccattctc tgcgcccagg agccgtacaa aaaacccgtc tccgtgggcg agccgccaga
     1201 ggatcactcg cccgccgccg agctggccac cttccaggtg ctggagggct ttgaggtgaa
     1261 tctcttcgcg gacgagtcgg acggcatcgc caacgcgctg gtcatccgct gggatgaaaa
     1321 aggccgcctt tggtgcctgc aaacctccgc ctatccccag cccgcgcccg atgaacctgc
     1381 ggatgataaa gtgatcatcc tggaggacac cgacggcgat ggccgggccg acaagagcac
     1441 cgtctgggcc tccggcctgc gcatgcccat gggcatggag ctggccgggc cggggcagct
     1501 ttatgtcggg gaaggggaga agctgctgct gctcaccgac acggatggcg acgaccgcgc
     1561 ggaccggcgg gaggtgattt tcagcggctt cggcaccggg gacacgcatc aaaacctgaa
     1621 cagcttcgtc tgggcgccgg agggctgcct ggtcatgcac caggggctgc actgctactc
     1681 gcgcgtggtc acgccgcacg gggtggcgca gctctacggc gcgggtttct ggcgttactg
     1741 gccgcgcagt cagcggctgg aggcctaccc caccggcatg ccgctgaacc cctggggcac
     1801 cgccttcacg cgggaaggcc agcccatcat ggtggcgggc gcggcgggca tgttctgggc
     1861 gcggcccatg gagatcgccg tgccggaggt gccgccggag caggcgggcg gcacgggcac
     1921 cacggcgggc ctgccgtatt tcctgctcac ccgccacatg ctgccgcaca gcggccagat
     1981 catcaagacg agcggcctgc ggaaattctg cggggtggac atcgccggcg gtccggcctg
     2041 gcccggcggc atgcatgggg aggtggtcac gggcggcttt ttcgagaacg ccgtttatcg
     2101 ccaccagctc atcgaggaca aggacgcgcc cagcggcttc gaagccgtgg agcagacacc
     2161 gctcatcacc agcagcagcg tggccttccg ccccgtggac gtgcgcttcg gccccgacgg
     2221 cgcgctgtac atcgccgact ggtacaaccc catcatcggc cactaccagg ccagcttccg
     2281 acacccgaac cgcgacaagc agcacgggcg catctggcgg gtggcggcga aagggcggga
     2341 gctgagcagg ccaaatctcg ccacaatggc atgggtcaga gagagttcca aatttgatca
     2401 gcaaaagacg gcgctgctgc tgtcacagac cgaaatcaaa ggcgatctct ggcttgccta
     2461 tcaagcagag agaattctgc gctttctgcc ggataaccac gcaatccgtg cggcgtcttg
     2521 ggagcttgga atagacgatt ccatggaaat tgtgatcccg cctgatgctc ctgagcaact
     2581 gaggcgggag cttgaggaag ttatcggcaa tcacaaagcc tgggttcatc agcaacatca
     2641 ccggatcctg cgcgcgatgg tttccttctc ggaaagtgtt caccttgaat atctccaaac
     2701 ctgcctcggc gacggaagat caacgattcg cgcatgggcc actcatagca tcggtgattg
     2761 tgcgcatcag ctgcccgatg tcctcgcctt gctggagccg cgcatcgccg atccccaccc
     2821 gcgcgtgcgg ctggaggcca tcgtcgcctg cgccaagctg aaatcggctg atgcgctgcg
     2881 gctggcgctg aaggcgctcg atgcgccgat ggacactttt ttggaccgcg cgctgtggct
     2941 cgccgtccat gccaccgcgc cgcagtggaa gcagccgggg actttggcgc ccttcctcgc
     3001 cgcgctgccg ccggagcacc tgagttatgt gctggaaaag gacggcagcc gtgagttgat
     3061 cgagaccgcg cgtgagatgc tcgccagcgg cgcggacacc ttgcctggtc ccacgcgcct
     3121 ggcgctgcac cgcatcctca tccgcaaggg cggtgtggaa gaccagatgc gcgcgctgca
     3181 ggcgggcatg gaggacatcg ccgtgctgac ggagctgaaa ctgacgctcg gcgcgcaggc
     3241 gaaggcaccg gcaccggagg gcgcgctgcc tttgctcctg gagctgctgg cctccgactc
     3301 cgaggaccgc cgcgccgccg cctgcgagct ggccgccgtg tggcgtctga tgcctg
//
LOCUS       DAOMYJ010000376         3355 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Prosthecobacter sp. isolate AMR_MDS_5629
            NODE_10293_length_3355_cov_18.869697, whole genome shotgun
            sequence.
ACCESSION   DAOMYJ010000376 DAOMYJ010000000
VERSION     DAOMYJ010000376.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867850
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prosthecobacter sp. (wastewater metagenome)
  ORGANISM  Prosthecobacter sp.
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Verrucomicrobiaceae; Prosthecobacter.
REFERENCE   1  (bases 1 to 3355)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3355)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.30
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:34:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,742
            CDSs (total)                      :: 4,684
            Genes (coding)                    :: 4,676
            CDSs (with protein)               :: 4,676
            Genes (RNA)                       :: 58
            tRNAs                             :: 55
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3355
                     /organism="Prosthecobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10293_length_3355_cov_18.869697"
                     /isolate="AMR_MDS_5629"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1965333"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..552)
                     /locus_tag="PLP58_21950"
     CDS             complement(<1..552)
                     /locus_tag="PLP58_21950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ11260.1"
                     /translation="MDSIPREAHLYVLIAALAVFFLLLALIRVILLRLAERKLLKANE
                     NLAAQLSVTKQDLATSRHESRAWREEMQRQFDAFRATADAHVSGERQRFDGLLHDSRL
                     REMELRASLEAARQMCAELPAAKARILQLEAMTAPAPKPQAPKPQAAKPPADDPPSGE
                     GGDGGAPVITPLPDLGGGGAPAPS"
     gene            complement(745..2433)
                     /gene="rpiB"
                     /locus_tag="PLP58_21955"
     CDS             complement(745..2433)
                     /gene="rpiB"
                     /locus_tag="PLP58_21955"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018970331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004751 - ribose-5-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009052 - pentose-phosphate shunt,
                     non-oxidative branch [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose 5-phosphate isomerase B"
                     /protein_id="HRJ11261.1"
                     /translation="MPDLPRTLAIGADHGGLSLKNEVAAHLRAGGYEVEDVGTHTPDS
                     VDYPDFAEAVAQRVLDGRADAGILVCTTGIGVCIAANRHAGIQASLVHDAETAAITRE
                     HNASNVLCLAGKTTPPATASQIVDAWLKTPFAGGRHGRRVDKMNTQAHPLSILASADP
                     AVAEIIRAEQHRQQTHIELIASENFTSRAVMAAQGSCLTNKYAEGYPAKRWYGGCEEV
                     DKVEQLAIDRACQLFGAKYANVQPHSGSQANAAVYFSVLQPGDKVLGMNLAHGGHLTH
                     GNPANFSGRFYDFCQYGVSPNDERIDYDELAGVALREKPKMITAGASAYPRVIDFARM
                     AEIAKSVGAYLFVDMAHIAGLVAGGAHPSPIEHADFITTTTHKSLRGPRGGLILTNRE
                     DLFKKLQSQVFPGIQGGPLMHVIAAKAVCFGECLNPDFKTYTRQIVKNAQALAAALAA
                     RGYRITSGGTDNHLMLVDLRPKGLNGKIASETLDLAGITVNKNGIPFDTEKITLGGGI
                     RVGTPAVTTRGMKEPEMELIAAWMDRALQSVNQPETLAQIKAEVAATNERFPLP"
     gene            complement(2470..2955)
                     /locus_tag="PLP58_21960"
     CDS             complement(2470..2955)
                     /locus_tag="PLP58_21960"
                     /inference="COORDINATES: protein motif:HMM:NF013607.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight protein arginine
                     phosphatase"
                     /protein_id="HRJ11262.1"
                     /translation="MKRVLFVCTGNTCRSPLAEALFRDLVKDRPDYTVQSAGVGAFSG
                     QPASRHSVQLARERGLDLSAHSSRAVTTGLVDEATHVFALSRSHLASLLMDYPEAGDK
                     FYLLSEFTADDRLRGRDVTDPFGGGIEEYRETLEILDQLLPSIHAYIEQTWKRAEPGS
                     Q"
BASE COUNT          463 a          938 c         1330 g          624 t
ORIGIN      
        1 ggacggggcg ggcgctccgc cgccgccgag gtcgggcagc ggagtgatca ctggcgcgcc
       61 gccatcgcca ccctccccgg atggcgggtc atccgcaggc ggtttggcag cctgcggctt
      121 cggggcttga ggtttcggag cgggggccgt catcgcttcg agctggagga tgcgcgcctt
      181 ggcggcgggc agttcagcgc acatctgccg ggcggcctcc agggaggcgc gtagttccat
      241 ctcgcgcagc cgggagtcgt gcagcaggcc gtcaaagcgc tgcctctcgc cgctgacgtg
      301 agcgtcagcc gtggcgcgga aggcgtcgaa ctggcgctgc atctcctcgc gccaggccct
      361 ggactcatga cggctcgtcg ccaggtcctg cttcgtcacg gagagttgcg cggccaggtt
      421 ttcattggcc ttgagcagct tcctttccgc cagacgcagc aggatcaccc ggatgagggc
      481 cagcaggagg aagaacaccg caagagcggc gatgaggacg taaagatggg cttcgcgcgg
      541 gatggaatcc ataattccca tgctaagcag gttcgtttcc gggaggcaat cttttttcca
      601 acggcggaaa atgatcccgg gttgcgactg aagcaggagc cttgtggtcc ccttggccgg
      661 ggattgcgcc ggcgcaggaa cagcgcgtcc ggcctgactt ggcggcgggc ggcagcgggt
      721 gccaggctgg caaggcccgc cggctcaggg cagcgggaag cgctcgttgg tggcggcgac
      781 ttcggctttg atttgggcga gcgtctccgg ctggttcacg ctttgcaggg cgcggtccat
      841 ccaggcggcg atgagctcca tctcgggctc cttcatgccg cgggtggtga cggcgggggt
      901 gccgacgcgg atgccgccgc cgagggtgat cttctcggtg tcaaagggga tgccgttttt
      961 gttcacggtg atgccggcga ggtcgagcgt ctcgctggcg atcttgccgt tgaggccctt
     1021 ggggcgcagg tccacgagca tgaggtggtt gtcggtgccg ccgctggtga tgcggtatcc
     1081 gcgcgcggcg agggcggcgg ccagggcctg ggcgtttttg acgatctgcc gggtgtaggt
     1141 cttgaagtcg gggttgaggc actcgccgaa gcagacggcc ttggcggcga tgacgtgcat
     1201 gagcgggccg ccctggatgc cggggaagac ctggctttgg agttttttga aaaggtcctc
     1261 gcggttggtg aggatgaggc cgccgcgcgg tccgcgcagg cttttgtgcg tggtggtggt
     1321 gatgaagtcg gcatgctcga tggggctggg atgcgcgccg ccggcgacga ggccggcgat
     1381 gtgggccatg tccacaaaga gataggcgcc gacgcttttg gcgatctcgg ccatgcgcgc
     1441 gaagtcaatg acacgcgggt aggcggaggc gccggcggtg atcatcttcg gcttctcgcg
     1501 cagggccacc ccggccagct cgtcgtagtc aatgcgctcg tcattcgggc tgacgccgta
     1561 ctggcagaag tcataaaagc ggccggagaa gttggcgggg ttgccgtggg tgaggtggcc
     1621 gccgtgggcg aggttcatgc ccagcacttt gtcgccgggc tggagcacgc tgaaatagac
     1681 ggcggcattg gcctgggagc cggagtgggg ctggacgttg gcgtatttgg cgccgaagag
     1741 ctggcaggcg cggtcaatgg cgagctgctc gaccttgtcc acctcctcgc agccgccgta
     1801 ccagcgcttg gcggggtagc cctcggcgta tttgttcgtc aggcaggagc cctgcgcggc
     1861 catgacggcg cggctggtga agttctcact ggcgatgagc tcgatgtggg tctgctggcg
     1921 gtgctgctcg gcgcggatga tttcggcgac ggcagggtcg gcggaggcga ggatgctgag
     1981 ggggtgggct tgggtgttca ttttatctac ccggcggcca tggcggccgc cggcaaaggg
     2041 tgtcttgagc cacgcgtcca cgatctggct ggcggtggcg ggcggggtgg ttttgccggc
     2101 caggcagagc acgttgctgg cattgtgctc gcgggtgatg gcggcggtct ccgcgtcatg
     2161 gacgaggctg gcctggatgc cggcgtggcg gttggcggcg atgcagacgc cgatgccggt
     2221 ggtgcagacg aggatgcccg cgtcggctct gccgtcgagc acgcgttgag cgacggcttc
     2281 agcaaagtcc ggataatcca ccgagtccgg cgtgtgagtg cccacatcct cgacctcgta
     2341 accgccggcg cgcaggtgcg cggcgacttc gtttttcagg ctcaggccgc cgtggtcggc
     2401 gccgatggcg agggtgcggg ggaggtcggg cattggggtg tttggagcgg gggaagggca
     2461 ggagccgggt cactggctgc cgggttcggc gcgtttccag gtctgctcga tgtaggcgtg
     2521 gatgctggga aggagttgat caaggatttc gagggtctcg cggtattcct cgatgccgcc
     2581 gccgaaaggg tcggtgacat cgcgtccgcg cagtctgtca tccgccgtga actcgctgag
     2641 aaggtagaat ttgtccccgg cctccgggta gtccatgagc agggaggcca ggtggctgcg
     2701 gctgagggcg aagacgtggg tggcttcgtc cacgaggccg gtggtgacgg cgcggctgct
     2761 gtgggcggag aggtccagtc cgcgctcccg ggccagttga acgctgtggc ggctggcggg
     2821 ctggccggag aaggcgccga ctccggcgct ctggacggtg tagtcgggac ggtccttgac
     2881 cagatcgcgg aaaagcgcct ccgccagggg gctgcggcag gtgtttccgg tgcagacaaa
     2941 gaggactcgt ttcaaaggac gcgcaggatg ccgcgccggg gggctttgtc aaaagcggag
     3001 agcctggaaa tgggcgcgcg gcctgcctta tcgtgcgcat gggtcgcccg ttcacttcgc
     3061 ggcttcgcag cagggctcca tcctcgcggg gcgcgtgacc cagggcgtcg ccctgggctg
     3121 ggatgggccg cgcctttggc gctttcggat cgaaggcgtc gtgccgggcc ggggcggcat
     3181 cctggagggg caggccgtcg gcgctgtgca gaagctgcca ggcatctgcg catctgcgcg
     3241 cgcggagtcg tgcggcaggt ggggaggctg ccttggcacg tttgcgaagt cgcgcggctt
     3301 gtggggaggc tctttttggg gcggaaaagg ctttggggct gcctccaggg ctgac
//
LOCUS       DAOMYJ010000377         3337 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Prosthecobacter sp. isolate AMR_MDS_5629
            NODE_10389_length_3337_cov_21.849482, whole genome shotgun
            sequence.
ACCESSION   DAOMYJ010000377 DAOMYJ010000000
VERSION     DAOMYJ010000377.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867850
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prosthecobacter sp. (wastewater metagenome)
  ORGANISM  Prosthecobacter sp.
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Verrucomicrobiaceae; Prosthecobacter.
REFERENCE   1  (bases 1 to 3337)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3337)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.30
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:34:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,742
            CDSs (total)                      :: 4,684
            Genes (coding)                    :: 4,676
            CDSs (with protein)               :: 4,676
            Genes (RNA)                       :: 58
            tRNAs                             :: 55
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3337
                     /organism="Prosthecobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10389_length_3337_cov_21.849482"
                     /isolate="AMR_MDS_5629"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1965333"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..646
                     /locus_tag="PLP58_21965"
     CDS             <1..646
                     /locus_tag="PLP58_21965"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HRJ11263.1"
                     /translation="ALELAARTADLPAPGERHEYSNFGYVLAGHMLEEVWRAPWEELM
                     RRRLFAPLGIQNAGFGGTGTPGKLDQPWPHNDKGEPMPGNGPLVDNPPVLGPAGTVHM
                     ALADWGLFLAEHLAGRAGRGRLLKTAAAYEHLHTAAQAGENYAFGWLASERPWGGRVL
                     NHAGSNTMNRSVTWLAPEKGFGVLACTNTGAETASKALDDVSGLLIQAHRQGGA"
     gene            complement(655..1500)
                     /gene="ftsY"
                     /locus_tag="PLP58_21970"
     CDS             complement(655..1500)
                     /gene="ftsY"
                     /locus_tag="PLP58_21970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009962129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005047 - signal recognition particle
                     binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HRJ11264.1"
                     /translation="MAGFFKSLLQRFTKPDIDWDELEAVLVTGDLGPRLSMRIVDDLK
                     AQGRKLHGADIVQVAREHVRRILPAQVTPLTPLEGRPKVVLIVGVNGTGKTTSTAKLA
                     KLLHDQGSKVVLAAADTFRAAAVEQLEVWSQRLGIPMVNGPPNGDPAAVCFEAYDLAC
                     RTGADFLLCDTAGRLHTKHNLMEELKKVHRILGRKDETSPHEVILVVDATTGGNALQQ
                     AREFHKAIPLTGVIVTKLDGSGKGGILVAIQQELGIPTRYIGLGEKAEDFQCFDPEKY
                     VAELL"
     gene            complement(1583..3004)
                     /gene="creC"
                     /locus_tag="PLP58_21975"
     CDS             complement(1583..3004)
                     /gene="creC"
                     /locus_tag="PLP58_21975"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF008312.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     CreC"
                     /protein_id="HRJ11265.1"
                     /translation="MSLTARFLLGFALVSTAGLYLLLARVLQRVERQYMEAAEEPMVD
                     VANIIAGILAAHSADGALDTAVLEKALGSAHQRRFEARIWSLLKERVDMHVYVTDAAG
                     RLLFDSAGIEPPGHPLGHRDVFLTLQGAYGARASRTDPADPLSVTMYVGAPVVAGGKT
                     LGMVSVAKPQRSLLAFIRETEAWLKTTLAGVVVFMLIGIYLVARWATRPLDDLARHAL
                     AVSRGERPAPPRMPGRQMKTLARALESMRDSLEGREYVEHYVQSLTHEMKSPVAAILG
                     ASEILQGDVPAPKRARFLQNIRAEAGRLRDLLERLLQLAALEKKKALETHESVDLRRA
                     LDRAWDHHALIAGARGITLHREMRHGLVIRGDSWLVELALGNLIQNAVDFSPDDGVIT
                     ARAYSFEDRAVMEIEDQGPGIPEYARERVFERFYSLPRPGTGKKSSGLGLCFVREVAQ
                     LHGGGIELLPVEGGLKAMLYLKG"
     gene            complement(3001..>3337)
                     /locus_tag="PLP58_21980"
     CDS             complement(3001..>3337)
                     /locus_tag="PLP58_21980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009965609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HRJ11266.1"
                     /translation="EPEAAPAAARGAPPLVVDETRCQVTYYGVPLALTRYEFRLLRAF
                     AAHPGRVFSREQLMERASEEPDASMERTVDTHIKTLRARLREARPGIDPIVTHRGMGY
                     SLLETWPAS"
BASE COUNT          502 a         1060 c         1242 g          533 t
ORIGIN      
        1 ggcgctggag ctggcggcgc gcacggcgga cctgcccgcg ccgggggagc ggcacgagta
       61 ctcgaacttc ggttacgtgc tcgccggcca catgctggag gaggtgtggc gcgccccgtg
      121 ggaggagctg atgcgccggc gcctctttgc cccgctgggc atccagaacg cgggcttcgg
      181 cggcaccggc acgcccggga aactggacca gccctggccg cacaacgaca aaggcgagcc
      241 catgccgggg aacggtcccc tggtggacaa cccccccgta ctcggccccg cgggcaccgt
      301 ccacatggcg ctggcggact ggggcctctt cctcgccgag cacctggccg ggcgcgcggg
      361 caggggccgg cttttgaaga ccgccgccgc ctacgagcac ctgcacaccg ccgcgcaggc
      421 tggggagaac tatgcctttg gctggctcgc cagcgagcgc ccgtggggag gccgggtgct
      481 gaaccacgcc ggcagcaaca ccatgaaccg cagcgtcacc tggctggccc cggagaaggg
      541 cttcggcgtg ctggcctgca ccaacacggg cgccgagacc gcctccaagg ccctcgatga
      601 tgtgtccggc ctgctcatcc aggcgcaccg ccagggcggc gcgtgaggct gcagtcacag
      661 cagctccgcc acatatttct ccgggtcgaa gcattgaaag tcctccgcct tttcccccag
      721 gccgatgtag cgggtgggga tgccgagttc ctgctggatg gcgacgagga tgccgccttt
      781 gccgctgccg tcgagcttgg tgacgatgac tccggtgagc gggatggctt tgtgaaactc
      841 ccgcgcctgc tggagcgcgt tgccgcctgt ggtggcatcc acgacgagga tgacctcatg
      901 cggcgaggtc tcatccttcc ggccgaggat gcggtggacc tttttcagct cctccatgag
      961 gttgtgcttg gtgtgcaggc ggccggcggt gtcgcacagg aggaagtcgg cgccggtgcg
     1021 gcaggcgagg tcgtaggcct cgaagcagac ggcggcgggg tcgccattcg gggggccatt
     1081 gaccatgggg atgccgaggc gctgggacca cacctcgagc tgctcgacgg cggcggcgcg
     1141 gaaggtgtcg gcggcggcga gcacgacctt gcttccctgg tcatggagga gcttggcgag
     1201 cttggcggtg gaggtggtct tgccggtgcc attgacgccg acgatgagca ccaccttcgg
     1261 ccggccctca agcggtgtga ggggtgtgac ctgcgcgggc aggatcctgc gcacatgctc
     1321 gcgcgcgacc tgcacgatgt cggccccgtg gagcttgcgc ccctgggctt tgaggtcgtc
     1381 cacgatccgc atggacagcc tcggccccag gtctccggtg acgagcacgg cctcgagttc
     1441 gtcccagtca atatcgggtt tcgtgaagcg ctggaggagg gatttgaaaa agccggccat
     1501 gagagtgtgt gcgggcgttt tgcataagca ggattcaccg gggtgccagt tttgattggg
     1561 cggacgaagc ggcaggcatg actcacccct tgaggtagag catggccttg agtccgccct
     1621 cgacaggcag caactcgata ccgccgccgt ggagttgggc gacttctcgg acgaagcaga
     1681 ggccgaggcc gctgctcttc ttgccggtgc cggggcgcgg cagggagtag aaacgctcga
     1741 acacgcgctc gcgggcgtat tccgggatgc ctggcccctg gtcctcgatc tccatgacgg
     1801 cgcggtcctc gaagctgtag gcgcgcgcgg ttatcacgcc gtcatccggg gagaaatcca
     1861 cggcgttttg gatcaggttg cccagtgcca gctcgacgag ccatgagtcg ccccggatga
     1921 cgagtccgtg gcgcatttcc cggtgaaggg tgatgccgcg cgcgccggcg atgagggcgt
     1981 ggtggtccca ggcgcggtcg agagcgcgcc gcaggtccac actctcgtgc gtttcgagcg
     2041 ccttcttttt ttcgagcgcg gccagttgca gcaggcgctc gagaagatcg cgcaggcggc
     2101 cggcctcggc gcggatgttc tggagaaagc gcgcgcgttt tggcgcgggc acgtcgccct
     2161 gcaggatttc ggaggctccg agaatggcgg ccacggggct tttcatctcg tgggtgaggc
     2221 tttgcacata gtgctccaca tactcgcggc cttcgaggga atcgcgcatg gactccaggg
     2281 cgcgggccag ggttttcatc tggcgcccgg gcatgcgcgg aggcgcgggt cgctcccccc
     2341 ggctgacggc gagggcgtgg cgggccaggt cgtcgagcgg ccgggtggcc cagcgcgcca
     2401 cgaggtagat gccgatgagc atgaagacca ccacgccggc gagggtggtc ttcagccagg
     2461 cctcggtctc gcggataaag gccaggaggc tgcgctgcgg cttggccacg ctgaccatgc
     2521 cgagggtctt gccgccggcc acgaccgggg cgcccacata catggtgacg gagagcgggt
     2581 cggcgggatc ggtacgggag gcgcgcgcgc cgtaggcgcc ctggagggtc aggaagacat
     2641 cccgatgacc gagcgggtgg ccgggcggct cgatgcctgc ggagtcgaaa agcaggcggc
     2701 ccgccgcgtc ggtcacatag acgtgcatgt ccacgcgctc cttgagcagg ctccagatgc
     2761 gcgcctcgaa gcgccgctgg tgcgcggagc caagcgcttt ttccagcacg gcggtgtcga
     2821 gcgcgccgtc cgcggaatgg gcggcgagga tcccggcgat gatgttcgcc acatccacca
     2881 tcggctcctc ggcggcctcc atgtactggc gctccacgcg ctgcagcacg cgcgcgagca
     2941 gcaggtagag gccggcggtg gagaccagcg cgaagccgag gaggaagcgc gcggtgaggc
     3001 tcatgaggcg ggccaggtct ccaggagtga ataacccatg ccgcggtggg tgacgatggg
     3061 gtcaatgccg gggcgcgcct ctcgcagccg cgcgcgcagg gtcttgatgt gcgtgtccac
     3121 ggtgcgctcc atggaggcgt ccggctcctc ggaggcgcgc tccatgagct gctcccggct
     3181 gaagacacgg cccggatgcg ccgcgaaggc gcgcagcagg cggaactcgt agcgtgttag
     3241 cgcgagcggg acgccgtagt aagtcacctg gcagcgggtc tcgtccacca ccagcggcgg
     3301 cgcgccgcgc gctgcggctg gcgcggcttc aggctct
//
LOCUS       DAOMYJ010000378         3321 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Prosthecobacter sp. isolate AMR_MDS_5629
            NODE_10472_length_3321_cov_18.469382, whole genome shotgun
            sequence.
ACCESSION   DAOMYJ010000378 DAOMYJ010000000
VERSION     DAOMYJ010000378.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867850
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prosthecobacter sp. (wastewater metagenome)
  ORGANISM  Prosthecobacter sp.
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Verrucomicrobiaceae; Prosthecobacter.
REFERENCE   1  (bases 1 to 3321)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3321)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.30
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:34:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,742
            CDSs (total)                      :: 4,684
            Genes (coding)                    :: 4,676
            CDSs (with protein)               :: 4,676
            Genes (RNA)                       :: 58
            tRNAs                             :: 55
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3321
                     /organism="Prosthecobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10472_length_3321_cov_18.469382"
                     /isolate="AMR_MDS_5629"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1965333"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1080
                     /locus_tag="PLP58_21985"
     CDS             <1..1080
                     /locus_tag="PLP58_21985"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013434.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HRJ11267.1"
                     /translation="TPRWRRRPPLSRSRPILIIGGGLAGTALAWRLHERGAPFLLVDR
                     DAPVTASKIAAGLVTPITGMRLTLSWRYSAFHAEALRFYRDKEALLGGVFFYETPVVR
                     LLRSEKEVHRWQERRAQPEIASFIHATREPLVNDAVFANPHGGFEQRPAGWLDTAAWL
                     QASRDFFTRQGAWLTGEVAADALQVNAGSASWDGREYESAVFCTGWEAARHPWFDWAP
                     FKSARGTILTLRADLGGERRVISSGCWVVPRADGSARAGPTYEIPFTAPHTPAREAVA
                     GLEAKLRAMVRADFQVIEEQTAVRPIVHGHEALLGLHPGRPRVAILNGLGSKGVLRSP
                     WLARHLVEHLLDGAPLEPAVDLAAH"
     gene            1140..3047
                     /locus_tag="PLP58_21990"
     CDS             1140..3047
                     /locus_tag="PLP58_21990"
                     /inference="COORDINATES: protein motif:HMM:NF040521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C45 family autoproteolytic
                     acyltransferase/hydrolase"
                     /protein_id="HRJ11268.1"
                     /translation="MKLRALLLASLVAIACLPSSARADALAAQKTLLSGVNQIMSVLA
                     PAPDSAPRTFSTTLRIVRATGLPQDVVGREAALAFQAPDKLRLSAKVGDETYSAGRNG
                     GRLWMHVAGKKFGVIGSPDAPRFKTDEGRPDGTKLPPLKLPVTREQLLLLPLLCSVET
                     LPDEAVLGELCAVLRLTPKSEARAAMNLPAATVTLWLRKSDDAPLRIGYADGRKVEVI
                     VELDGLALEPAWPDSAWNLQAGPEDKIETVALGHLTRFLPAALDMLGQKLPPLGPATG
                     ERRVLARHGKGRLEMRDGTRVLFLEGMPEEIGAQHGTLMKKEVHHLVRRILYGVGVGS
                     SFGKGRWFFGEIEEAQSRLQPFVSGRHLREMDALAAAAGVRVEEARLANFFPELFHCS
                     GFAVFGDATEGGRMYHGRILDYMKGVGLEQNACVIVMRPDEGHAWVNLGYAGFLGTVT
                     AMNEKRIAIGEMGGRGEGAWDGKPMAQLMREVMEKASTIDEAVEIMRSSPRTCEYYYV
                     ISDGNTRRAVGIAATPEKFETIWAGASHPQLPHPVKDTVLMSAGDRYEALVSRVREGY
                     GRFDADRARDLMTRPVCMTSNIQSVLFAPETLDFWVANADGEYVASHTRYTAYNLAEL
                     LKPEAPAREGE"
BASE COUNT          533 a         1207 c         1062 g          519 t
ORIGIN      
        1 actcctcgat ggcggcgacg gccgcccttg tctcgttcgc gccccatcct catcatcggc
       61 ggcgggctcg ccggcaccgc gctggcgtgg cggctgcatg agcgcggcgc gcccttcctg
      121 ctcgtggacc gcgacgcgcc cgtcacagcc tcgaagatcg ccgccggact cgtcactccc
      181 atcacgggaa tgcgcctgac gttaagctgg cgatactccg cctttcacgc cgaggcgctg
      241 cgtttttacc gggacaagga ggccctgctc ggcggcgtgt ttttttacga aacccccgtc
      301 gtacgcctgc tgcgcagtga gaaagaggtc caccgctggc aggagcgccg tgcccagccg
      361 gagatcgcgt ccttcattca tgccacccgc gagccgctgg tgaatgacgc cgttttcgcc
      421 aacccccacg gcggcttcga gcaaaggccc gccggatggc tcgacaccgc cgcctggctc
      481 caggccagcc gcgacttttt cacccgccag ggcgcctggc tcacgggcga ggtggccgcc
      541 gatgccctcc aggtgaatgc cggctcggcg agctgggatg gccgcgagta tgagagcgcg
      601 gtcttttgca caggctggga ggctgcccgc cacccgtggt tcgactgggc acccttcaag
      661 tccgcgcgcg gcaccatcct caccctgcgc gcggatctcg gcggggagcg gcgcgtcatc
      721 agctccggct gctgggtcgt gccgcgcgcc gacggcagcg cgcgcgccgg acccacttat
      781 gagataccct tcaccgcgcc gcacactccc gcgcgcgagg ccgtggccgg cctggaggca
      841 aaactgcgcg ccatggtgag ggcggacttc caggtcatcg aagagcagac cgccgtgcgc
      901 cccatcgtcc acgggcacga ggccctgctc ggcctccacc ccggccggcc gcgcgtggcc
      961 atcctcaacg gcctcggctc caaaggcgtg ctgcgctcac cctggctcgc gcggcatctc
     1021 gtggagcacc tgctggatgg agctccgctg gagcctgcgg tggatctggc ggcacattga
     1081 gagtgccgtg gaattatttg agattgccca ctgcaccggg cgcggagtaa aaccgccgca
     1141 tgaaactccg cgcgctcctt ctcgccagcc tcgtcgccat cgcctgcctg ccgtcatccg
     1201 cgcgcgccga cgctctggcc gcgcagaaga cgctgctctc cggcgtgaat caaatcatgt
     1261 ccgtgctggc tccggccccg gactcggctc cgcgcacctt ctccaccacg ctgaggatcg
     1321 tccgcgccac gggcctgccg caggacgtgg tcgggcgcga ggcggccctg gctttccaag
     1381 cgcctgacaa gctgcgcctc agcgccaaag tcggcgatga aacctacagc gccggacgca
     1441 acggcggccg cctctggatg catgtcgcgg ggaaaaaatt cggcgtcatc ggctcgccgg
     1501 acgcgccgcg tttcaagacg gacgagggcc gcccagacgg caccaagctg ccgccgttga
     1561 agctgcccgt cacgcgcgag caactgctcc tgctgccgct gctctgctcc gtggagacac
     1621 tgccggatga ggccgtgctc ggcgaactct gcgccgtgct gcgcctcaca cccaagagcg
     1681 aggcgcgcgc cgccatgaac ctgcccgccg ccactgtcac cctctggctg cgcaagagcg
     1741 acgacgcgcc gctgcgcatc ggttatgcgg acggcaggaa ggtggaagtc atagtcgagc
     1801 tggacggcct cgcgctggag cccgcctggc cggacagcgc ctggaacttg caagccggac
     1861 cggaggacaa gatcgagacc gtcgcgctcg gccacctcac tcgttttctc cccgccgcgc
     1921 tcgacatgct cggccaaaaa ctgcccccgc tcggacccgc caccggcgag cgccgcgtgc
     1981 tggcccggca tggcaaaggg cggctggaaa tgcgcgacgg cacgcgcgtg ctcttcctcg
     2041 aaggcatgcc cgaggagatc ggcgcgcagc acggcacgct catgaagaag gaggtgcatc
     2101 acctcgtgcg gcgcatcctt tacggagtcg gcgtcggcag ctcctttggc aaagggcgct
     2161 ggtttttcgg cgagatcgag gaggcgcaat cccggcttca gcccttcgtc agcgggcgtc
     2221 atctgcgcga gatggatgcc ctggccgccg cggcgggagt gcgtgtcgag gaggcccggc
     2281 tggcgaactt tttccccgag ctcttccatt gcagcggctt cgcggtcttt ggcgacgcca
     2341 ccgagggcgg gcgcatgtat cacgggcgca tcctcgacta catgaagggc gtgggcctgg
     2401 agcaaaacgc ctgtgtcatc gtcatgcgcc cggacgaggg ccacgcctgg gtgaacctcg
     2461 gctacgccgg cttcctcggc accgtcaccg ccatgaacga gaagcgcatc gccatcggcg
     2521 aaatgggcgg gcgcggcgag ggcgcgtggg acggcaaacc catggcgcag ctcatgcgcg
     2581 aggtgatgga aaaagcctcc accattgacg aggccgttga gatcatgcgc tccagcccgc
     2641 gcacctgcga gtattactat gtgatctccg acggcaacac gcgccgcgcc gtcggcatcg
     2701 ccgccacgcc ggagaaattc gagaccatct gggccggtgc cagccacccg cagctcccgc
     2761 atcctgtcaa agacaccgtg ctcatgtccg ccggcgaccg ctacgaagcg ctcgtctccc
     2821 gcgtgcgcga gggttacggg cgcttcgacg cggaccgcgc gcgcgatctc atgacgcgcc
     2881 ccgtgtgcat gacctcgaac atccagtccg tgctcttcgc gcccgaaacg ctcgacttct
     2941 gggtcgcgaa cgccgacggt gaatacgtcg ccagccacac ccgctacacg gcttacaacc
     3001 tcgccgagct gctcaagccg gaggcgcctg ccagggaagg tgagtgactc caccgggcgg
     3061 cgggcttcac gcgccccgcg caaaatccac cgccatttca tcccggcttc atcaagactt
     3121 cacacacctc ttgaggatgc tcggtgatga aaccgctcat cctcctgctg ctgggagccg
     3181 gccttgcgcc cgctcaaaac tcatcccgtg tcacctccgc gtcactcgac gacagcgagg
     3241 tgcgcatcac ctacggcgag ctgaagagcc tcgtcaccgc cgcgcagccc gcgccatccg
     3301 ccgcggagcc gaagcccaag c
//
LOCUS       DAOMYJ010000379         3310 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Prosthecobacter sp. isolate AMR_MDS_5629
            NODE_10513_length_3310_cov_19.652227, whole genome shotgun
            sequence.
ACCESSION   DAOMYJ010000379 DAOMYJ010000000
VERSION     DAOMYJ010000379.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867850
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prosthecobacter sp. (wastewater metagenome)
  ORGANISM  Prosthecobacter sp.
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Verrucomicrobiaceae; Prosthecobacter.
REFERENCE   1  (bases 1 to 3310)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3310)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.30
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:34:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,742
            CDSs (total)                      :: 4,684
            Genes (coding)                    :: 4,676
            CDSs (with protein)               :: 4,676
            Genes (RNA)                       :: 58
            tRNAs                             :: 55
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3310
                     /organism="Prosthecobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10513_length_3310_cov_19.652227"
                     /isolate="AMR_MDS_5629"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1965333"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1362
                     /locus_tag="PLP58_21995"
     CDS             <1..1362
                     /locus_tag="PLP58_21995"
                     /inference="COORDINATES: protein motif:HMM:NF024545.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HRJ11269.1"
                     /translation="GRTYTVAEAQRLSRRLNVTYMLQLFDLLSALSRSGGQADAAINQ
                     VILQTELERLGVMPDDAEAQAAMEKLPAFMENGVFSQQRAYNAQQMLGSYGLSAQDML
                     DIMKLSIGHAKLKDLAGKNYVAGPLEVEKSYASEHQTLKVATISFSLEDQKKAAVVTD
                     EEIQKYYDENKETYKTPEKRAVSYVFFENPKEDDKKPLEERQKAQREVVDRVNKFNEA
                     SIAPGASFDAIVKDLKEAELKAPLFERESPPDAIKDEADLIAAIFAHNNQTRPVSDPV
                     KGAKGYYIFTVTQTEEPRQQELGEVKDKIKETLTAQKAQEGLTKAVNDARDALAAGLK
                     EGKKIEDLAKELKLTLTPVTDLTIFAPPAEMPHAYEIARAARDTPAGGLTQSIDTDTG
                     ALLAHVQAKELRKREDSAALRENMAATRASMEQNRLFEAWFSKLREAAKPRTLLKEAE
                     EQA"
     gene            1369..1719
                     /locus_tag="PLP58_22000"
     CDS             1369..1719
                     /locus_tag="PLP58_22000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018971121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HRJ11270.1"
                     /translation="MSQPAPASVDDIFDEASGHLALGEMDQAVALYRRCVQQDPRFFD
                     GWHALGMALLKTGEVKEAIGAGLMATTLRPNDLLAWTGLSQMYVRAGMIAEAEDAKGK
                     ARILSLGGKVAREA"
     gene            1762..2544
                     /gene="trpC"
                     /locus_tag="PLP58_22005"
     CDS             1762..2544
                     /gene="trpC"
                     /locus_tag="PLP58_22005"
                     /EC_number="4.1.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009962094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004425 - indole-3-glycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006568 - tryptophan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="indole-3-glycerol phosphate synthase TrpC"
                     /protein_id="HRJ11271.1"
                     /translation="MNKLDEIIAHKRTEVEKLLPRLDKLRAAATRRNDTRSLADALRA
                     DPTRLGMIAEIKRASPSAGTIAADFDPLAIARGYEKAGATALSVLTDEKYFQGRLEYL
                     SLIRSQVDIPCLRKDFIIHEAQIYEASVAGADAILLIVAALDQPELEHLLETAHTFQL
                     DALVEVHDLPELERALATDARIIGVNNRNLKTFTVDLATTEALAEEVPDDIILVSESG
                     IKTPADAARLAEAGADALLVGETLMRSANILADLPALRAPRP"
     gene            complement(2593..>3310)
                     /locus_tag="PLP58_22010"
     CDS             complement(2593..>3310)
                     /locus_tag="PLP58_22010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ11272.1"
                     /translation="PPPAAAGRGGLRDMLANPQMRAVMDQQQAMQIEMSYARLFQHLG
                     LNDQEKEHFKKLLTGRQKAMMDTSLKLMDPNLTPEQRQALSQEVEKQKTAFDQTIKAF
                     LNNEDDWQTFQQWEATLPERQMFDTLGRGLFSSSAEPLSAQQEQQLIQLMAGVRASPG
                     RGGNLMGKTNMDPAKMTPELINEMVSQLQVNAQVVEERAAQFLTPAQLQTLKAYHKQT
                     IEMSKSGIEMSRMLMQGKGQ"
BASE COUNT          644 a         1283 c          904 g          479 t
ORIGIN      
        1 ggccgcacct acaccgtggc cgaggcccag cgcctcagcc gccgcctcaa cgtgacttac
       61 atgctccagc tcttcgacct gctctccgcc ctcagccgct ccggcggcca ggccgacgcg
      121 gccatcaacc aggtcatcct ccaaaccgaa cttgagcgcc tcggagtcat gcccgacgac
      181 gccgaggccc aggccgccat ggaaaagctg cccgccttca tggagaacgg cgtcttcagc
      241 cagcagcgcg cctacaacgc ccagcagatg ctcggctcct acggtctcag cgcccaggac
      301 atgctcgaca tcatgaagct cagcatcggc cacgccaagc tcaaggacct cgccggcaaa
      361 aactacgtcg ccggccctct cgaagtcgaa aagagctacg ccagcgagca ccagaccctc
      421 aaggtcgcca ccatcagctt ctccctcgaa gaccagaaga aggccgccgt cgtcaccgac
      481 gaggagatcc agaagtatta cgacgagaac aaggagacct acaaaacccc cgagaagcgc
      541 gccgtcagct acgtcttctt tgagaacccc aaagaggacg acaaaaagcc cctggaagag
      601 cgccagaaag cccagcgcga ggtcgtggac cgggtcaaca agttcaacga ggccagcatc
      661 gcccccggag ccagcttcga cgccatcgtc aaagacctca aggaggccga actcaaagcc
      721 ccccttttcg agcgcgaatc cccccccgac gccatcaagg acgaagccga cctcatcgcc
      781 gccatcttcg cccacaacaa ccagacccgc cccgtcagcg accccgtcaa aggcgccaag
      841 ggctactaca tcttcaccgt cacccagacc gaggagccca ggcagcagga actcggagaa
      901 gtgaaggaca agatcaagga aaccctcacc gcccagaaag cccaggaagg actcaccaag
      961 gcagtcaacg acgcgcgcga cgccctcgcc gccggactca aggagggcaa aaaaatcgag
     1021 gatctcgcca aggaactcaa actcaccctc acccccgtca ccgacctcac catcttcgcc
     1081 ccgcccgccg aaatgcccca tgcctacgag atcgcccgcg ccgcgcgtga cacccccgcc
     1141 ggcggcctca cccagagcat agacaccgac accggcgccc tgctcgccca cgtccaggcc
     1201 aaggagctgc gcaagcgcga ggacagcgcc gccctgcgtg aaaacatggc cgccacccgc
     1261 gcctccatgg agcagaaccg cctcttcgaa gcctggttct ccaagctgcg cgaggccgca
     1321 aagccccgca ccctcctcaa agaggccgaa gagcaagcct gacccctcat gagccagccc
     1381 gccccggcca gcgtggacga catcttcgac gaggccagcg gccacctcgc cctcggggag
     1441 atggatcagg ccgttgccct ctaccgccgc tgcgtccagc aggatccgcg gttcttcgac
     1501 ggctggcacg ccctcggcat ggccctgctg aaaaccggcg aggtcaaaga agccatcggg
     1561 gccggcctca tggccaccac cctccgcccc aacgacctcc tcgcctggac cggcctctcc
     1621 caaatgtatg tgcgcgccgg catgatcgcc gaggccgagg acgccaaggg caaggcccgc
     1681 atcctctccc tcggaggaaa ggtcgccagg gaggcatgag gcgcttgcgc ccccgccgca
     1741 gcgccccatc ttccgcgccc catgaacaag ctggacgaga tcatcgcgca caagcgcacc
     1801 gaggtggaaa agctcctgcc gcgcctggac aagctccgcg ccgccgccac ccgcaggaat
     1861 gacacccgct ccctcgccga cgccctgcgg gcggatccca cccgcctcgg catgatcgcc
     1921 gaaatcaagc gcgcctcccc ctccgccggc accatcgccg ccgacttcga ccccctcgcc
     1981 atcgcccgcg gctacgagaa agccggcgcc accgccctct ccgtcctcac tgacgaaaaa
     2041 tacttccagg gccggctcga atacctcagt ctcatccgct cccaggtgga catcccctgc
     2101 ctgcgcaagg acttcatcat ccacgaggcc cagatttacg aggcctccgt cgccggggcg
     2161 gacgccatcc tcctcatcgt cgccgccctc gaccagcccg aactcgagca cctcctggag
     2221 accgcccaca ccttccagct cgacgccctc gtcgaagtcc acgacctgcc cgaactcgag
     2281 cgcgccctcg ccaccgacgc ccgcatcatc ggcgtcaaca accgcaacct caagaccttc
     2341 accgtggacc tcgccaccac cgaggccctg gccgaggaag tgcccgacga catcatcctc
     2401 gtctccgaaa gcggcatcaa gacacccgcc gacgccgccc gcctcgccga agccggagcc
     2461 gacgccctcc tcgtcggcga gaccctgatg cgcagcgcca acatcctcgc agacctcccc
     2521 gccctccgcg cccccaggcc ctgagcgccc cctcggccac atcactagcc agcgggcagg
     2581 cgcgcgctgc cctcactgcc ccttcccctg catgagcatg cgggacattt cgatgccgga
     2641 cttcgacatc tcgatggtct gcttgtggta ggccttgagt gtctgcaact gcgcgggggt
     2701 gaggaactgc gcggcgcgct cctcgacgac ttgcgcgttc acctggagct ggctgaccat
     2761 ctcgttgatc aattccggcg tcatcttcgc cgggtccatg ttcgtcttgc ccatgaggtt
     2821 ccccccgcgt cccggcgagg cgcgcacgcc cgccatcaac tggatgagct gctgctcctg
     2881 ctgggcgctg agcggctcgg cggaggagct gaaaaggccc cgtccaagag tgtcgaacat
     2941 ctgccgctcc ggcagggtgg cctcccactg ctggaaggtc tgccagtcgt cctcgttgtt
     3001 gaggaaggcc ttgatggtct ggtcaaaggc ggtcttttgc ttctccacct cctggctcag
     3061 cgcttggcgc tgctcgggcg tgaggttcgg gtccatgagc ttgaggctgg tgtccatcat
     3121 ggccttttgc cgcccggtga gcagcttttt aaaatgctcc ttttcctggt cgttgaggcc
     3181 gaggtgctgg aacagtcgcg cgtaggacat ctcgatctgc atggcctgct gctggtccat
     3241 caccgcgcgc atctgggggt ttgccagcat gtcccgcagt ccgcccctgc ctgcggcggc
     3301 gggcggcggc
//
LOCUS       DAOMYJ010000380         3303 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Prosthecobacter sp. isolate AMR_MDS_5629
            NODE_10554_length_3303_cov_17.849138, whole genome shotgun
            sequence.
ACCESSION   DAOMYJ010000380 DAOMYJ010000000
VERSION     DAOMYJ010000380.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867850
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prosthecobacter sp. (wastewater metagenome)
  ORGANISM  Prosthecobacter sp.
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Verrucomicrobiaceae; Prosthecobacter.
REFERENCE   1  (bases 1 to 3303)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3303)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.30
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:34:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,742
            CDSs (total)                      :: 4,684
            Genes (coding)                    :: 4,676
            CDSs (with protein)               :: 4,676
            Genes (RNA)                       :: 58
            tRNAs                             :: 55
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3303
                     /organism="Prosthecobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10554_length_3303_cov_17.849138"
                     /isolate="AMR_MDS_5629"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1965333"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1059)
                     /locus_tag="PLP58_22015"
     CDS             complement(<1..1059)
                     /locus_tag="PLP58_22015"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007412814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HRJ11273.1"
                     /translation="MPDTLQSFVEEAFRELAADDAPTLPLEERLCGGENAGDVIGRYT
                     LVEKLGEGGFGSVWRAEQERPVRREVALKIIKLGMDTREVIARFEVERQALAVMEHPG
                     IAKVFDAGATVSGRPFFVMELVRGTPLTQFCEEAAMPLPERLELFIQVCQAVQHAHQK
                     GVIHRDLKPSNILVTGADGRPLPKVIDFGIAKAAAGTPLTDNTLVTRADRLMGTPAYM
                     SPEQSETGMDLDTRTDIYSLGVLLYELLTGQVPFAAGPDGKSTRGRDPQRPSTRIRSF
                     TAEQMRRLAAGRGTDGPRLIGQVKGDLDWIAMKALEADRARRYDSANALAEDLRAFLE
                     RRPVQARPPTAWYLARRFA"
     gene            complement(1104..2219)
                     /locus_tag="PLP58_22020"
     CDS             complement(1104..2219)
                     /locus_tag="PLP58_22020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009959602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HRJ11274.1"
                     /translation="MKALVLTGPCEFNYDIAFPDPAPAAGEVLVKVKACGICGSDIHG
                     MDGRSGRRQMPIVMGHEAAGEIVALGEGVTGWEIGERVTFDSTEYCGECEECRSGYVN
                     LCPNRKVLGVSPGEYRRHGCFAEKIALPARILYRIPEALTFEKAAFAEPVSIALHAVN
                     LADGIEVGEAFTQGADCAEGCECHDEDAEGHGGTAVVVGAGLIGLLVVQALKARGWER
                     VIAVDLDDKRLALARELGAAETFNACQEGLAMHLRQLCGGDGADASFEVVGAAAPLDL
                     AIRCVRKGGQVILVGNLQPNTPFPLQEVVTRQLTLRGSCSCAGEYPEAIRRIEDGSIR
                     VEPLLSAVAPLQEGADWFQRLYDNKEGLLKVVLTPEA"
     gene            complement(2279..>3303)
                     /locus_tag="PLP58_22025"
     CDS             complement(2279..>3303)
                     /locus_tag="PLP58_22025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ11275.1"
                     /translation="PAAAASGPAPAAEKEWPRSVAIADKPEVVVVKEDAEAKKFIYRS
                     PHYEFECDSRLGANVVREFGRLFEATYLLNCMLPLDLKPKPEPLREYFLARLYTNEED
                     YMKSGGAPGSAGVYQRGEKALSVPLKSLGVKMVGSRVSLDKTDDDANATLIHEITHQM
                     MNHWLARLPTWFIEGSAEYPTLIEYFPTGRFSWTGIRRRLETYAAQKNYYEPMPFKML
                     DLQELMEIKSAAWSAALTREIQAPRVAGGVATQSSQNYGSAGLLTYYFYHHDGNGDAG
                     NVIAWLREVEAAERGTDFAALMKKHLLRERTYAQLAADVKKGLNKAGIDVVFEPPGKN
                     PAATAE"
BASE COUNT          483 a         1163 c         1109 g          548 t
ORIGIN      
        1 ggcgaagcgc cgcgccaggt accacgccgt gggcgggcgc gcctgcacgg gacgccgctc
       61 caggaaggcg cgcaggtcct ccgccagcgc gttggccgag tcgtaacggc gcgcgcggtc
      121 ggcctccagc gccttcatgg cgatccagtc caggtccccc ttcacctgcc cgatgagccg
      181 cgggccgtcc gtgccgcggc ctgcggccag ccggcgcatc tgctcggcgg tgaaggagcg
      241 gatgcgcgtc gagggccgct gcggatcgcg cccgcgcgtg cttttcccat ccggcccggc
      301 ggcgaagggc acctgccccg tcagcagctc gtagagcagc acgccgaggc tgtagatgtc
      361 cgtgcgcgtg tccaggtcca tgcccgtctc ggactgctcc gggctcatgt aggcgggcgt
      421 gcccatgagg cggtccgcgc gcgtcaccag cgtgttgtca gtcagcggcg tgccggccgc
      481 ggccttggcg atgccgaagt caatgacctt cggcagcggc ctgccgtccg ccccggtgac
      541 gaggatgttc gagggcttca agtcacggtg gatcacgccc ttttgatgcg cgtgctgcac
      601 cgcctggcag acctggatga aaagctccag cctctccggc agcggcatgg ccgcctcctc
      661 gcagaactgc gtcagcggcg tgccgcgcac cagctccatg acaaaaaacg gccgcccgct
      721 caccgtcgcc cccgcgtcga acaccttggc gattcccgga tgctccatca ccgccagcgc
      781 ctgccgctcc acctcgaagc gcgcgatgac ctcccgcgtg tccatgccca gcttgatgat
      841 cttcagcgcc acctcccgcc gcaccggccg ctcctgctcc gcgcgccaca ccgagccgaa
      901 gcccccctcc cccaacttct ccaccagcgt gtagcgcccg atgacatccc ccgcgttttc
      961 cccgccgcag agcctctcct ccaatggcag cgtcggcgcg tcatccgccg ccagctcgcg
     1021 gaacgcctcc tcgacgaatg actgcaatgt gtccggcatg ggcgcacaca tactctccgc
     1081 gcctccgcgc gcaaggcgcg cgctcacgcc tccggcgtca gcaccacttt cagcaggccc
     1141 tctttgttgt cataaagccg ctggaaccag tccgcgccct cctggagcgg cgccacggcg
     1201 ctgagcaggg gctccacgcg gatgctgccg tcctcgatgc ggcggatggc ctcgggatac
     1261 tcgccggcgc aggagcagga gccgcgcagg gtgagctggc gggtgacgac ttcctgcagc
     1321 gggaagggtg tgttcggctg gaggttgccc accaggatca cctggccgcc tttgcgcacg
     1381 cagcggatgg cgaggtccag cggggccgcc gcgccgacga cctcgaagct ggcgtccgcg
     1441 ccatcgccgc cgcagagctg gcgcaggtgc atggccaggc cttcctggca cgcgttgaaa
     1501 gtctccgccg cgcccagctc gcgggccagg gccaggcgct tgtcatccag gtccacggcg
     1561 atcacccgct cccagccgcg cgccttgagc gcctgcacca cgagcaggcc gatcaagccc
     1621 gcgccgacca cgacggcggt gccgccgtgg ccttccgcgt cctcatcgtg gcactcgcag
     1681 ccttcggcgc agtccgcgcc ctgggtgaag gcctcgccca cctcgatgcc gtccgccagg
     1741 ttcactgcgt gcagggcgat ggacacgggc tcggcaaagg cggctttttc aaaggtcagc
     1801 gcctccggga tgcggtagag gatgcgcgcg ggcagggcga tcttttcggc gaagcagccg
     1861 tgccgccggt actcgccggg ggagacgccg agcaccttgc ggttcgggca gagattcaca
     1921 taaccggagc ggcactcctc gcactcgccg cagtattcgg tggagtcgaa agtgacgcgc
     1981 tcgccgatct cccagccggt gacgccctcg ccgagggcga cgatttcccc cgccgcctca
     2041 tgacccatga cgatgggcat ttgacggcgg ccgctgcgcc cgtccatgcc gtggatgtcg
     2101 ctgccgcaga tgccgcaggc tttgactttc accagcacct cgccggccgc gggcgcggga
     2161 tcggggaagg cgatgtcgta gttgaattcg caggggccgg tgaggacgag ggctttcatg
     2221 gtggcgctga attggcgcag agcgcccgcg caggcaagca cgaagcggtg cggcagcctt
     2281 actccgccgt ggcggccggg ttcttccccg gcggctcgaa gacgacatca atgccggctt
     2341 tgttgagccc cttcttcacg tcggcggcaa gctgcgcgta ggtgcgctcg cggagcaggt
     2401 gcttcttcat gagggcggcg aaatccgtgc cgcgctccgc ggcctccacc tcgcgcagcc
     2461 aggcgatgac attgccggcg tcgccgttgc cgtcgtgatg atagaagtaa taggtgagca
     2521 gccccgccga gccgtagttc tggctggact gggtggccac gccgcccgcc accctgggcg
     2581 cctgaatctc gcgtgtgagc gcggcgctcc aggcggcgct cttgatctcc atgagctcct
     2641 ggagatcgag catcttgaag ggcatcggct catagtagtt cttctgcgcg gcataggtct
     2701 ccaggcggcg gcggatgccg gtccaggaaa acctgcctgt ggggaagtat tcgatgagcg
     2761 tggggtactc ggcgctgccc tcgatgaacc aggtgggcag gcgcgccagc cagtggttca
     2821 tcatctggtg ggtgatctcg tgaatgaggg tggcattggc gtcgtcgtcg gtcttgtcga
     2881 gggagacacg gctgccgacc attttgacgc cgaggctttt gagcggcacg gagagcgcct
     2941 tctcaccgcg ctggtaaacc cccgcggagc cgggcgcgcc gccactcttc atgtagtcct
     3001 cctcgttggt gtagagccgg gccaggaaat actcgcgcag cggctcgggc ttcggcttga
     3061 ggtccagggg cagcatgcag ttcaaaagat aggtcgcctc aaacaggcgc ccgaactcgc
     3121 gcaccacatt cgcgccgaga cgcgagtcgc actcgaactc gtagtgcggg gagcggtaga
     3181 tgaatttttt cgcctcggcg tcctctttga ccaccaccac ctcgggcttg tcggcgatgg
     3241 cgacgctgcg cggccattcc ttctccgccg ccggtgccgg gccggacgcg gcggcggccg
     3301 ggg
//
LOCUS       DAOMYJ010000381         3302 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Prosthecobacter sp. isolate AMR_MDS_5629
            NODE_10557_length_3302_cov_26.812134, whole genome shotgun
            sequence.
ACCESSION   DAOMYJ010000381 DAOMYJ010000000
VERSION     DAOMYJ010000381.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867850
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prosthecobacter sp. (wastewater metagenome)
  ORGANISM  Prosthecobacter sp.
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Verrucomicrobiaceae; Prosthecobacter.
REFERENCE   1  (bases 1 to 3302)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3302)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.30
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:34:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,742
            CDSs (total)                      :: 4,684
            Genes (coding)                    :: 4,676
            CDSs (with protein)               :: 4,676
            Genes (RNA)                       :: 58
            tRNAs                             :: 55
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3302
                     /organism="Prosthecobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10557_length_3302_cov_26.812134"
                     /isolate="AMR_MDS_5629"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1965333"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..376
                     /locus_tag="PLP58_22030"
     CDS             <1..376
                     /locus_tag="PLP58_22030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ11276.1"
                     /translation="QRVAEQARYFMVKDVETITRTGREAAAFVADVLKRKPFNVLTRW
                     ERVPNTLRYYAIIVVEGDDGRPVYLADLLMRRGYARVSGVDTFLPGDARDVPTYMAEL
                     QSLARKARETKSGIWGKAGPPE"
     gene            512..>3302
                     /locus_tag="PLP58_22035"
     CDS             512..>3302
                     /locus_tag="PLP58_22035"
                     /inference="COORDINATES: protein motif:HMM:TIGR02604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FG-GAP-like repeat-containing protein"
                     /protein_id="HRJ11277.1"
                     /translation="MKRALLSLLLPALLQAETHITWKRVQLQDQFYSEGANYADINKD
                     GHADIISGSYWYEGPDWQRKHAFYEPKVFNIFGYSDNFFCYAHDFTGNGWLDILVIGF
                     PGKEARLYLNPGRFDEDKPWPMHIVAEVVDGESPVFSDITGDGKPEIVCGNGGRFGWF
                     APDWSNPTAKWPFVPVTENVQVAKFTHGMGVGDVDGDGKLDLLEAKRWWRQGAGVANS
                     GADLASPAPWQPHTFALGVPGGAQMFAYDFDGDGRNDIFTTLAAHRHGVAVFFNRAGQ
                     GGASWERKLIIGEHPWENDYGVVFSQPHAAHLADMDGDGVMDVVTGKRYWAHNGRDAG
                     EHAAKVLYWLQTKRDGKGGVEFIPHLISADCGVGVDVQVGDVNGDGFPDVIVGNKAGV
                     FVLLQERQEVSQEVAEQMRPKKMYGEGLMPYGEYKEGQPAAEALKNLQLPGGFKAELI
                     AAEPDVVQPIAMCWDERGRLWVVEGNSYPKPREVGAGQDRILIFEDSNGDGTFETRKV
                     FAEGLNLVSGIELGFGGVWVGAAPYLMFIPDRDRDDKPDVPDAADPRPKVPGLKFTAY
                     ALLDGWGSQDTHETLNSFIWGPDGWLYGCHGVFTHSRVGKPGTPDAQRVPINAGVWRY
                     HPVRHEFEVYAHGTSNPWGLDYDQHGEFFVTACVIPHLYHIVPGGRYHRQAGQHFNPH
                     TYEDIKTIADHAHFAGDVRENAHWGPRSKGGIISDDTNMMGGGHAHCGLAIYQSSQFP
                     ATYRNQLIFGNLHGHRLVMDELDPKASTYIGKHGSDFLRANDLHFIPVSQRVGPDGAL
                     YVSDWADKQICHRGSNAMEMWDRSNGRIYRVSYTTSSDRSDLSDFPTAPFDLSKETDV
                     KLAQLAVQTENEWFSRMARRVLMQKAVERTLAGVSGADYPSWMKVMLETRGAAQVEVS
                     LRSLWIELTLSSIGGG"
BASE COUNT          626 a          991 c         1090 g          595 t
ORIGIN      
        1 ccagcgcgtg gcggagcagg cccgctattt catggtgaag gatgtcgaaa ccatcacgcg
       61 cacaggcagg gaggccgccg cctttgtggc ggatgtgctc aagcgcaagc ccttcaacgt
      121 gctgacgcgc tgggagcggg tgccgaacac cttgcgctac tatgccatca tcgttgtgga
      181 gggcgacgac ggcaggccgg tttatctggc ggacctcctc atgcggcgcg gttatgcgcg
      241 tgtgtccggc gtggacacct tcctgccggg cgacgcccgc gatgtgccga cctacatggc
      301 cgagctgcaa tccctggccc gcaaggctcg cgagacaaag tccggcatct ggggcaaggc
      361 cggcccgcct gagtagtgag gagttttgga gtcaaagtga agcgccatcg cccgtcaccc
      421 gagggcttcg cttccgctcc ttcactgtcc gatcttttat aaaacttgtc cgccatcagg
      481 cctttctggt agcgttggcc taattccaac catgaaacgc gcgctcctct ccctcctcct
      541 gcccgccctg ctccaggcgg agacccacat cacctggaag cgggtgcaac tgcaggacca
      601 gttttactca gaaggcgcca actacgccga catcaacaag gacggccacg cggacatcat
      661 cagcggctct tactggtatg aggggccgga ctggcagagg aagcacgctt tctacgagcc
      721 taaggtcttc aacatcttcg gctattcgga caactttttt tgttatgccc acgacttcac
      781 gggcaacggg tggctggaca tcctggttat cggcttcccc ggcaaggagg cgcggcttta
      841 tctgaatccg ggccgttttg acgaggacaa gccctggccg atgcacatcg tggccgaggt
      901 ggtggatggc gagtcgcccg tgttctccga catcacgggc gatggcaagc cggagatcgt
      961 ctgcggcaat ggcggacgct ttggctggtt cgcgccggat tggagcaacc cgacggcaaa
     1021 gtggcccttc gtgcccgtga cggagaatgt gcaggtggcc aaattcaccc acggcatggg
     1081 tgtgggggat gtggatggcg atggcaaact ggacctgctg gaggcgaagc gctggtggcg
     1141 gcaaggagcc ggagttgcca actccggcgc agacttggca agtccggctc cttggcaacc
     1201 ccacaccttc gcgctcggcg tgccgggcgg ggcgcagatg ttcgcctatg attttgatgg
     1261 cgacgggcgc aatgacatct tcaccacgct ggcggcgcat cggcatgggg tggctgtgtt
     1321 tttcaatcgc gcgggccagg gcggggcgag ctgggagcgc aagctcatca ttggcgagca
     1381 tccttgggag aatgattacg gcgtggtctt cagccagccg cacgcggcgc atctggcgga
     1441 catggatggc gatggtgtga tggatgtggt cacgggcaag cgctactggg cgcacaacgg
     1501 gcgcgacgcg ggcgagcacg cggccaaggt gctctactgg ctgcaaacca agcgcgatgg
     1561 caaaggcggc gtggaattca tcccgcacct catcagcgcg gactgcggcg tgggcgtgga
     1621 tgtgcaggta ggggatgtga atggcgacgg cttcccggat gtgatcgtgg gcaacaaggc
     1681 gggcgtcttt gtcctgctcc aggagcggca ggaggtgagc caggaggtgg cggagcagat
     1741 gcggcccaag aaaatgtatg gcgagggtct catgccctac ggcgagtaca aggaagggca
     1801 gccggcggcg gaggccttga agaatctgca actgcccggc ggcttcaaag ccgagctgat
     1861 cgccgccgaa ccggatgtgg tgcagcccat cgccatgtgc tgggacgagc gcggccgcct
     1921 gtgggtggtg gagggcaaca gctatccgaa gccgcgcgag gtgggcgcgg gacaggaccg
     1981 cattttgatc tttgaggata gcaatggcga cggcaccttc gagacgcgca aggtcttcgc
     2041 cgaagggctg aacctggtca gcggcatcga gctgggcttc ggcggtgtgt gggtcggtgc
     2101 cgcgccgtat ttgatgttca tcccggaccg ggaccgcgat gacaaacccg acgttcctga
     2161 tgctgccgat ccacggccaa aagtccccgg cctcaaattc accgcttacg cgctgctcga
     2221 tggctggggc agccaggaca cgcatgagac gctgaacagc ttcatctggg ggccggacgg
     2281 ctggctttac ggctgccacg gcgtcttcac ccacagccgc gtgggcaagc ctggcacgcc
     2341 ggacgctcag cgcgtgccga tcaatgcggg cgtttggcgg tatcatccgg tgcggcatga
     2401 gttcgaagtc tatgcgcacg gcaccagcaa cccgtggggg ctcgattacg accagcacgg
     2461 cgagtttttc gtcaccgcct gtgtcatccc gcatctctac cacatcgtgc ctggcgggcg
     2521 ctaccaccgg caggcggggc agcacttcaa tccgcacact tacgaggaca tcaagaccat
     2581 cgccgaccac gcgcacttcg ccggcgacgt gcgtgagaac gcgcactggg gaccgcgcag
     2641 caagggcggc atcatctccg acgacacgaa catgatgggc ggcggccacg cccactgcgg
     2701 cctggcgatt tatcagagca gccagttccc cgccacctac cggaaccagc tcatcttcgg
     2761 caatctgcac ggccaccgcc tcgtgatgga cgagctggac ccgaaggcca gcacctacat
     2821 cggcaagcac ggcagcgatt tcctgcgcgc caacgacctg cacttcatcc ccgtcagcca
     2881 gcgcgtcggt cccgacggcg cgctctacgt cagcgactgg gcagacaaac aaatctgcca
     2941 ccgcggcagc aacgccatgg aaatgtggga ccgcagcaat gggcggatct accgggtgag
     3001 ttacacgact tcgtcggaca ggtccgacct gtccgacttc cccaccgcgc cgtttgatct
     3061 ctctaaagag acggatgtga aactcgccca actcgccgtg cagacggaga acgagtggtt
     3121 ctcgcggatg gcacggaggg tgttgatgca gaaggctgtg gaacgcacac tcgctggcgt
     3181 ttctggtgcc gactacccat cgtggatgaa ggtaatgctc gaaacacgag gtgccgcaca
     3241 agtggaagtc tcactcagat ccttgtggat tgagttgact ttatcttcta tcggaggcgg
     3301 gg
//
LOCUS       DAOMYJ010000382         3256 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Prosthecobacter sp. isolate AMR_MDS_5629
            NODE_10770_length_3256_cov_18.513590, whole genome shotgun
            sequence.
ACCESSION   DAOMYJ010000382 DAOMYJ010000000
VERSION     DAOMYJ010000382.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867850
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prosthecobacter sp. (wastewater metagenome)
  ORGANISM  Prosthecobacter sp.
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Verrucomicrobiaceae; Prosthecobacter.
REFERENCE   1  (bases 1 to 3256)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3256)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.30
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:34:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,742
            CDSs (total)                      :: 4,684
            Genes (coding)                    :: 4,676
            CDSs (with protein)               :: 4,676
            Genes (RNA)                       :: 58
            tRNAs                             :: 55
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3256
                     /organism="Prosthecobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10770_length_3256_cov_18.513590"
                     /isolate="AMR_MDS_5629"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1965333"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(266..961)
                     /gene="pseF"
                     /locus_tag="PLP58_22040"
     CDS             complement(266..961)
                     /gene="pseF"
                     /locus_tag="PLP58_22040"
                     /EC_number="2.7.7.81"
                     /inference="COORDINATES: protein motif:HMM:TIGR03584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070567 - cytidylyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudaminic acid cytidylyltransferase"
                     /protein_id="HRJ11278.1"
                     /translation="MSALAIILARSGSKRIPGKNLRPFLGHPVIHYPVKAALDSGCFD
                     EVMVSTDDDQIAAAARAAGAVTPFRRSAENASDQATTTDALLEVIAQYRAAGCEFEFI
                     CGMYASAPLITVDHLRAGWEIIKDNPAAQTVMPVIRYGYPVHRAFVIQQGRLQLMMPE
                     HGFTRSQDLPVAYHDAGQWYWMRVADFERGRKVFADRCLPVELSELEVQDIDNEVDWK
                     LAELKARIQRGLL"
     gene            complement(989..2164)
                     /gene="pseC"
                     /locus_tag="PLP58_22045"
     CDS             complement(989..2164)
                     /gene="pseC"
                     /locus_tag="PLP58_22045"
                     /EC_number="2.6.1.92"
                     /inference="COORDINATES: protein motif:HMM:TIGR03588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-4-amino-4,
                     6-dideoxy-N-acetyl-beta-L-altrosamine transaminase"
                     /protein_id="HRJ11279.1"
                     /translation="MSAPFLPYGRQSLDEADIAAVVEVLKSDYLTQGPAIPRFEKAVA
                     HWCGARHGIAMANGTATLHCAAKALGLGHGDRLWTSPVTFVASANAGRYCGAKIDFVD
                     VDPGTILMCPDRLEAKLVEAEKHGTLPKVVVPVHFAGQSCDMPRIHALSEKYGFQILE
                     DAAHALGGEYQGQRIGNCRWSHAASHSFHPVKIITSGEGGMITTNDDELAWRIGLLRT
                     HGITREPGRMTGESHGPWYYQQLELGFNYRMTDLQAALGASQMTRLDAFARRRREIAD
                     LYDRELSHLPLRPLACDPRGISGWHLYMIRLDLEAIRPRTRRQVFESLRAQGIGVNVH
                     YIPVHLQPDYQRLGFTAGMFPDAERYYEEAVTLPMFPAMTDGDIARVRAALEAALAA"
     gene            complement(2161..>3256)
                     /gene="pseB"
                     /locus_tag="PLP58_22050"
     CDS             complement(2161..>3256)
                     /gene="pseB"
                     /locus_tag="PLP58_22050"
                     /EC_number="4.2.1.115"
                     /inference="COORDINATES: protein motif:HMM:TIGR03589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 4,6-dehydratase
                     (inverting)"
                     /protein_id="HRJ11280.1"
                     /translation="IFRLQTSPLPPPVFSKNSRSTPTPDPVLKSQSILVTGGTGSFGH
                     KCVARLLADPDVKRVVIYSRDELKQFEMAQRFTDKRVRFFIGDVRDRERLTHAMSGID
                     TVIHAAALKQVPTAEYNPFECIKTNVLGAENVVQACLDAGVTNVVALSTDKAAAPINL
                     YGATKLCSDKLFIAANNVRGWSKIRFSVVRYGNVMGSRGSVIPFFLEKRKTGVLPITD
                     MQMTRFNITLEEGVDLVMHALEHALGGEIFVPRIPSYRIGDVAEAIGPQCQHPVVGIR
                     PGEKIHEEMITETDALQTIETGRHYIIVPMLYGHTAASLMERYCAAHQGAPVAAGFSY
                     NSGKNTEWLTVAQIRELIRQHCDPSFTVDA"
BASE COUNT          511 a          829 c         1325 g          591 t
ORIGIN      
        1 agattttgcc cggggcggca aaagcggctg cgcttgggcg gttgggatgc gtcggggccg
       61 acgtgccgcg cgccctggcc tgaggtccag cggcatttat accaagcgtc atcacaaatg
      121 atgaaaccag caattggatt catggagtgc tgggcgtcga atagccatca ttccacatct
      181 ccagcactcc aggattggat tcacaaaatc gtgatgacgc gcggcatggc cgtgctcgtt
      241 cccaaacagg gtctggcggt tcctgttaga gaagtcctct ttggatgcgg gctttgagct
      301 cggcgagctt ccagtccact tcgttgtcta tgtcctggac ttcgagctcg gagagctcga
      361 cggggaggca gcggtcggcg aagactttgc ggccgcgctc gaagtcggcg acgcgcatcc
      421 agtaccactg gccggcgtcg tggtaggcga cggggaggtc ctgggagcgg gtgaagccgt
      481 gctcgggcat catgagctgg aggcggccct gctggatgac gaaggcgcgg tgcacggggt
      541 agccgtagcg gatgacgggc atgacggttt gcgcggcagg gttgtctttg atgatctccc
      601 agccggcgcg caggtggtcc acggtgatga ggggggcgga ggcgtacatg ccgcagatga
      661 actcgaactc gcacccggcg gcgcggtatt gggcgatgac ttcgagcagg gcgtctgtgg
      721 tggtggcctg gtcgctggcg ttttccgcgg agcgccggaa gggggtgacg gctccggcgg
      781 cgcgggcggc ggcggcgatt tggtcgtcgt cggtggagac catgacttcg tcaaagcagc
      841 cggagtcgag ggcggctttg acggggtagt ggatgacggg gtggccgagg aaggggcgga
      901 ggtttttgcc ggggatgcgc ttgctgccgc tgcgggcgag gatgatggcg agggcggaca
      961 tgggaggcgg tgaatgcggc gcaccgtgtc acgcggcgag ggcggcttca agcgcggcgc
     1021 ggacgcgggc gatgtcgccg tcggtcatgg cggggaacat gggcagggtg acggcctcct
     1081 cgtagtagcg ctcggcatcg gggaacatgc cggcggtgaa gccgaggcgc tggtagtcgg
     1141 gctggaggtg gacggggatg tagtggacgt tgacgccgat gccctgggcg cgcaggcttt
     1201 cgaagacttg gcggcgggtg cgcgggcgga tggcttcgag gtcgagccgg atcatgtaaa
     1261 gatgccagcc gctgatgccg cgcgggtcgc aggcgagggg gcggaggggg aggtgggaga
     1321 gctcgcggtc gtagaggtcg gcgatctcgc gccggcggcg ggcgaaggcg tcgagccggg
     1381 tcatctggct ggcgccgagg gcggcctgga ggtcggtcat gcggtagttg aagccgagct
     1441 cgagctgctg gtaataccag gggccgtggg attcgccggt catgcggccg ggctcgcggg
     1501 tgatgccgtg ggtgcgcagc aaaccgatgc gccaggcgag ctcgtcgtcg ttggtggtga
     1561 tcatgccgcc ctcgccgctg gtgatgattt tgacggggtg gaagctgtgg gaggcggcgt
     1621 gggaccagcg gcagttgccg atgcgctggc cctggtattc gccgccgagg gcgtgggcgg
     1681 cgtcttcgag gatttggaag ccgtattttt ccgagagtgc gtggatgcgc ggcatgtcgc
     1741 aggactgccc ggcgaagtgg acggggacga cgactttggg cagagtgccg tgtttttcgg
     1801 cttcgacgag ctttgcctcc aggcggtccg ggcacatgag gatggtgccg gggtccacgt
     1861 ccacgaagtc tatcttcgcg ccgcagtagc gcccggcatt ggcggaggcg acgaaggtga
     1921 cgggggaggt ccaaagccgg tcgccatggc cgaggccgag ggctttggcg gcgcagtgga
     1981 gggtggcggt gccgttggcc atggcgatgc cgtggcgggc gccgcaccag tgggcgacgg
     2041 ctttttcaaa gcgggggatg gcgggcccct gggtgaggta gtcggacttc aggacctcga
     2101 cgacggcggc gatgtcggcc tcgtcgaggc tctggcgtcc gtagggcagg aagggggcgc
     2161 tcatgcgtcc acggtgaagc tcgggtcaca atgctggcgg atgagctcgc ggatctgggc
     2221 gacggtgagc cattcggtgt ttttgccgga attgtaggag aagccggcgg ccacgggcgc
     2281 gccctggtgg gcggcgcagt agcgctccat gagactggcg gcggtgtggc cgtagagcat
     2341 ggggacgatg atgtagtggc ggccggtctc gatggtctgg agggcgtcgg tctcggtgat
     2401 catctcctcg tggatcttct cgccgggacg gatgccgacg acgggatgct ggcactgcgg
     2461 gccgatggcc tcggccacgt caccgatgcg gtaggagggg atgcggggga cgaaaatctc
     2521 ccctccgagc gcgtgctcca gcgcgtgcat gacgaggtcc acgccctcct cgagggtgat
     2581 gttgaagcgc gtcatctgca tgtccgtgat gggcagcacg ccggtcttgc gcttctccag
     2641 aaagaagggg atgacggagc ctcgggagcc catgacattg ccgtagcgga cgacgctgaa
     2701 gcggattttg ctccagccgc ggacgttgtt ggcggcgatg aaaagcttgt ccgagcagag
     2761 cttggtggcg ccgtagaggt tgatgggggc ggcggccttg tcggtggaaa gggcgacgac
     2821 gttggtgacg ccggcgtcga ggcaggcctg gacgacgttt tcggcgccga ggacgttggt
     2881 cttgatgcac tcgaaggggt tgtactcggc ggtgggcacc tgcttgagcg cggcggcgtg
     2941 gatgacggtg tctatgcccg acatggcgtg cgtgaggcgc tcgcggtcgc gcacgtcgcc
     3001 gatgaagaag cgcacgcgct tgtcggtgaa gcgctgggcc atttcaaact gcttcaactc
     3061 gtcgcgcgag tagatgacca cgcgcttgac atcgggatcg gcgagcaggc gggcgacgca
     3121 tttgtggcca aaggaaccgg tgcctccggt gacaaggatg gactgggatt tcaagacggg
     3181 atcaggggtg ggagtggagc gagagttctt cgagaagact gggggaggca agggggaagt
     3241 ttgaagtctg aaaatc
//
LOCUS       DAOMYJ010000383         3219 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Prosthecobacter sp. isolate AMR_MDS_5629
            NODE_10950_length_3219_cov_14.670354, whole genome shotgun
            sequence.
ACCESSION   DAOMYJ010000383 DAOMYJ010000000
VERSION     DAOMYJ010000383.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867850
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prosthecobacter sp. (wastewater metagenome)
  ORGANISM  Prosthecobacter sp.
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Verrucomicrobiaceae; Prosthecobacter.
REFERENCE   1  (bases 1 to 3219)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3219)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.30
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:34:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,742
            CDSs (total)                      :: 4,684
            Genes (coding)                    :: 4,676
            CDSs (with protein)               :: 4,676
            Genes (RNA)                       :: 58
            tRNAs                             :: 55
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3219
                     /organism="Prosthecobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10950_length_3219_cov_14.670354"
                     /isolate="AMR_MDS_5629"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1965333"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(43..1002)
                     /locus_tag="PLP58_22055"
     CDS             complement(43..1002)
                     /locus_tag="PLP58_22055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009966017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ThuA domain-containing protein"
                     /protein_id="HRJ11281.1"
                     /translation="MIRRLILLAALLSLTAAHAAPKKLLVVTVTTGFRHSSIETAEKV
                     LAQLGATSETYTVDYVRQPEGGQPKSPGRPPERKPKESDAEFKARQEAYSTALAAFNT
                     ANAAWNDTVKAHMAAHMSLDKLRAYDGFIFANTTGDLQLPDRDGFIQLIREGRAFIAM
                     HSGGDTYHPFRGYVDMLGGEFLTHKQQVEVQPTLHDPAHPATQPVPSGWRVFDEIYIF
                     KSYDPATVHALMGLNEHPNDKTPGYYPVSWCKEFGKGKVFYTSLGHREDVWDPTWKEG
                     TSERKNSPEIARTYQAHILGGIRWALGLQPGSAEPGNVKAAAP"
     gene            complement(1075..>3219)
                     /locus_tag="PLP58_22060"
     CDS             complement(1075..>3219)
                     /locus_tag="PLP58_22060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ11282.1"
                     /translation="KDRAAAEWVALGQAALALGADAQKVIQQYFAVAQKKDPKLEAAW
                     LAEGNLALEKNDAKRAADVFRAGLKAHGETAALRLGLARAFAGSDGEKERENLARALE
                     INPVLAEAHLMRAEGLIRAEKFLDAEAAIQKVLDLRENHPEAWGLRAAIARLSAADEK
                     KFQSARAGGLQLWDKNPAVDHAIGRILSRAYRFAEGSTHQRRALEMDPDYLPAKVQLC
                     HDLLRLGEEDEAWKLAAAIREQDGYNVQAHNVGRLEQQMRGFTTESFEDFIIKMPKRE
                     WPVYGERALALLREAKAALCAKYGLELRRPVIVEFFDAQQDFAIRTFGSLGGQGLLGV
                     CFGTVITMNSPGSMAHGRNNWESTLWHEFCHVVTLTLTKNKMPRWLSEGVSVHEEALR
                     DPAWGMKMNADFRRMILEDGDATPVSQLSGAFMSPESPDHLSFAYFQSSLVVDHIVDR
                     FGHQALLGILRDLASGKKINDAISANTGPVEELEKDFERHLKTLARAYGPDADWSEPE
                     PEQLNPLDPASLAAFREKHPANLHALRLDLKNLTAKEQWAEALVLAEELARLVPGDFS
                     GEGGHAMKAFLLKKLDRRDDELAVQRHIAASEPSAMPVFLRLIEHDEQAQDWPQVRLN
                     AARAMALNPFLTTPQRALAAAAEALGENDTAAAALQRLLLLDPASAARSHYRLALLLR
                     ESDAAQAKRHLLESLALAPRFREGHALLRDWP"
BASE COUNT          409 a          905 c         1317 g          588 t
ORIGIN      
        1 ggaccaagtg ccctaccccg gagcggcgcg ggtttggatg gctcagggcg cggcggcttt
       61 cacgttgccg ggctcggcgc tgccgggctg gaggccgagg gcccagcgga tgccgccgag
      121 gatgtgggcc tggtaggtgc gggcgatctc gggggagttt ttgcgctcgc tggtgccctc
      181 cttccacgtc gggtcccaga cgtcttcgcg gtggccgagg gaggtgtaga agactttgcc
      241 tttgccgaac tccttgcacc aggagacggg gtagtagccg ggggtcttgt cattcgggtg
      301 ctcgttgagg cccatgaggg cgtggacggt ggcggggtcg tagcttttga agatgtagat
      361 ctcgtcgaac acgcgccagc cggagggcac gggctgggtg gcggggtggg cggggtcgtg
      421 cagggtgggc tggacttcga cctgctgctt gtgggtgagg aattcgccgc cgagcatgtc
      481 cacgtagccg cggaaggggt ggtaggtgtc gccgccggag tgcatggcga tgaaggctct
      541 gccttcgcgg atgagctgga tgaagccgtc gcggtcggga agctggaggt cgccggtggt
      601 gttggcgaag atgaagccgt cgtaggcgcg gagcttgtcg aggctcatgt gcgcggccat
      661 gtgggctttg acggtgtcgt tccaggcggc gttggcggtg ttgaacgcgg cgagggcggt
      721 gctgtaggcc tcctggcggg ccttgaactc ggcgtcgctt tccttgggct tgcgctcggg
      781 ggggcgtccg gggcttttcg gctggccgcc ctcgggctgg cggacgtaat ccacggtgta
      841 ggtctcggag gtggcgccga gctgggcgag gactttttcg gcggtctcga tggaggagtg
      901 gcggaagccg gtggtgacgg tgacgacgag gagcttcttc ggcgcggcgt gggcggcggt
      961 gagggagagc agggcggcga ggaggatgag gcggcggatc atgggtgcgg gctgggtgtt
     1021 agcatgacgg caggcggcgg cggcttcttg cctgcaaatg cgcgcgccgg cgggttacgg
     1081 ccagtctctg agcagggcgt ggccttcgcg gaagcggggc gcgagggcga ggctttccag
     1141 gagatgcctc ttggcctggg cggcgtcgct ttcgcgcagc aggagcgcga ggcggtagtg
     1201 gctgcgggcg gcgctggcgg ggtcgagaag caggaggcgc tggagggcgg cggcggcggt
     1261 gtcgttttct ccgagggcct cggcggcggc ggcgagggcg cgctggggtg tggtgaggaa
     1321 ggggttcagg gccatggcgc gcgcggcatt gaggcggacc tggggccagt cctgcgcctg
     1381 ctcgtcgtgc tcgatgaggc ggaggaagac gggcatggcg gagggttcgc tggcggcgat
     1441 gtggcgctgg acggcgagct cgtcgtcgcg gcggtcgagc tttttgagga ggaaggcctt
     1501 catggcgtgg ccgccctcgc cgctgaagtc cccgggcacg aggcgggcga gctcttcggc
     1561 gaggacgagg gcctcggccc actgctcttt ggcggtgagg tttttcaagt cgaggcgcag
     1621 ggcgtggagg ttggcggggt gcttttcgcg gaaggcggcg agggaggcgg ggtcgagcgg
     1681 gttgagctgc tccggctccg gctcgctcca gtcggcgtcg gggccgtagg cgcgggcgag
     1741 ggtcttgagg tggcgctcga agtccttctc cagctcctcc accggcccgg tgttcgcgga
     1801 gatggcgtcg ttgatctttt tgccggaggc gagatcgcgc aggatgccga gcagcgcctg
     1861 gtgcccgaag cggtccacga tgtggtccac gacgaggctg gactggaagt aggcgaagga
     1921 gaggtgatcg ggcgactcgg gactcatgaa ggcgccgctg agctggctga cgggggtggc
     1981 gtcgccgtcc tcaaggatca tgcggcggaa gtcggcgttc attttcatgc cccaggcggg
     2041 gtcgcgcagc gcctcctcat gcacgctgac gccctcgctg agccagcgcg gcattttgtt
     2101 tttcgtgagg gtgagggtga cgacgtggca gaactcatgc cagagggtgg actcccagtt
     2161 gttgcgcccg tgggccatgc tgccggggct gttcatggtg atgacggtgc cgaagcagac
     2221 gcccagcagg ccctggccgc cgaggctgcc gaaggtgcgg atggcgaagt cctgctgcgc
     2281 gtcgaagaac tcgacgatga cgggccgccg cagctccagg ccgtacttcg cgcagagggc
     2341 ggcctttgcc tcgcgcagca gggccagggc gcgctcgcca tagacgggcc actcgcgttt
     2401 gggcatcttg atgatgaagt cttcgaagct ctcggtggtg aagccgcgca tctgctgctc
     2461 gagcctgccg acattgtgcg cctgcacatt gtagccgtcc tgctcgcgga tggcggcggc
     2521 cagcttccaa gcctcgtcct cctcgccgag gcggagcagg tcatggcaga gctgcacctt
     2581 ggccgggaga taatccgggt ccatctccag ggcgcggcgc tggtgcgtgg agccttcggc
     2641 aaagcggtag gcgcgggaaa ggatgcggcc gatggcgtgg tccacggcgg ggttcttgtc
     2701 ccacaattgc aggccgccgg cgcgcgcgct ctggaacttc ttttcgtcgg cggcgctgag
     2761 ccgggcgatg gccgcgcgca gtccccaggc ttcgggatgg ttctcgcgca ggtcgaggac
     2821 cttctggatg gcggcctcgg cgtcgaggaa cttctccgcg cggatgaggc cctcggcgcg
     2881 catgaggtgc gcctcggcga ggacggggtt gatctccagg gcgcgggcga ggttctcgcg
     2941 ctccttctcg ccgtcgctgc cggcaaaggc gcgagccagt ccgaggcgga gagccgccgt
     3001 ctcgccatgc gccttgaggc cggcgcggaa gacatcggcg gcgcgtttgg cgtcgttttt
     3061 ctccagggcc aggttcccct cggcgagcca ggcggcctcc agcttcggat ccttcttctg
     3121 cgccacggcg aagtattgct ggatgacctt ttgcgcgtca gcgccgagcg ccagcgcggc
     3181 ctggccgagc gccacccact cggcggcggc gcggtcctt
//
LOCUS       DAOMYJ010000384         3146 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Prosthecobacter sp. isolate AMR_MDS_5629
            NODE_11325_length_3146_cov_19.652863, whole genome shotgun
            sequence.
ACCESSION   DAOMYJ010000384 DAOMYJ010000000
VERSION     DAOMYJ010000384.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867850
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prosthecobacter sp. (wastewater metagenome)
  ORGANISM  Prosthecobacter sp.
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Verrucomicrobiaceae; Prosthecobacter.
REFERENCE   1  (bases 1 to 3146)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3146)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.30
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:34:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,742
            CDSs (total)                      :: 4,684
            Genes (coding)                    :: 4,676
            CDSs (with protein)               :: 4,676
            Genes (RNA)                       :: 58
            tRNAs                             :: 55
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3146
                     /organism="Prosthecobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11325_length_3146_cov_19.652863"
                     /isolate="AMR_MDS_5629"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1965333"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..232
                     /locus_tag="PLP58_22065"
     CDS             <1..232
                     /locus_tag="PLP58_22065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ11283.1"
                     /translation="SVVPEAPALGAVHDAGGGWSRATWYSQPWYGPWQGGFISGHGGW
                     PAYPGWGVCAPRYRVSGSYHGRAGGVIFSIGR"
     gene            complement(233..1624)
                     /gene="ffh"
                     /locus_tag="PLP58_22070"
     CDS             complement(233..1624)
                     /gene="ffh"
                     /locus_tag="PLP58_22070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009961691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0048501 - signal recognition particle,
                     plasma membrane targeting [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005048 - signal sequence binding
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HRJ11284.1"
                     /translation="MFSALTDKLEDAFRKLRGLGKISESNVADALREIRLALLEADVD
                     FKVAKDLVESVKARALGEEVLRNVSPGQQIVKIFHDELVKLLGGGAQELDLSPPQRIL
                     LAGLNGAGKTTTSAKLAAWLKKQGRRPLLVALDLYRPAAITQLQVLGQQLGAPVFAPA
                     AGETDVVRSAKAALAWLDQQGAGIAIFDTAGRQEVDEALLAELKAVRDLVQPRETLLV
                     ADAATGQQAVSVASKFHEAIGITGLIMSKLDGDARGGALLSMRQVTGCPVKFIGVGEK
                     TDQLEVFHPDRMAQRILGMGDVVSLVEKAAQEIDEKEAMSMMRRLEQNKFDFNDFLGQ
                     LRFMKKLGPLEGLLGMIPGMGKLKGMPGVDDKRMRHVEAIILSMTPKERARPEILNGS
                     RRKRIAKGAGRPVVELNQLLKQFEMMRKLMKNKGAMAQMAGGLLGGGGMPQMPKGMFP
                     GGKMPKMPKGFRF"
     gene            1709..>3146
                     /locus_tag="PLP58_22075"
     CDS             1709..>3146
                     /locus_tag="PLP58_22075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ11285.1"
                     /translation="MKANPRFLLTALFAATALSLQGTDLYSIGQPTDEEQFYLELINR
                     ARANPAAEGARLAAATDPDVLSAIATFNVNLTVMQNEFNALPVRPPMAFNLKLMLAAR
                     GHSQDMFNNVFQGHDGSNGSTLSQRVTAQGYAFSSLGENVFSFASSVFHGHAGFQIDW
                     GEEGDGTGMQIGRGHRTSIHSARREVGIGMVLGSKTQGATTVGPQLVTQDFGTGNPDN
                     QAFVTGVAFYDLNGNSFYDPGEGVGGLTVNVAGSAFHAVTANSGGYTVPVPTTNTTRA
                     VTFAGLGLNGGADAVISGGANVKVDFAPAYSPPVLSGPVDIALNEAATHSFTTVGGAT
                     GYELRAVPDAPVPVDGAENLGLVTAATTGAYTPRSTTVKHAGTSAYRLTHPAVAVSKE
                     RLTYNAAYLVQSGGALSFRSRLRLASADQTAQVQVSTDGGFTWQTVFSQSGTGGAGEG
                     SFQLRNISLAAFEGREIRVRFEYAFPGGS"
BASE COUNT          469 a         1011 c         1121 g          545 t
ORIGIN      
        1 cagcgtggtg ccggaggcgc cggcgctggg cgcggtgcat gacgcgggcg ggggctggag
       61 ccgcgccacc tggtattcgc agccctggta cgggccgtgg caggggggat tcatcagcgg
      121 ccacggcggc tggcctgcct atccgggctg gggcgtgtgc gcgccgcgct accgcgtcag
      181 cggcagctat cacgggcgcg cgggcggggt gattttcagc atcgggcgct gatcagaagc
      241 ggaagccttt gggcatcttg ggcattttgc cgccggggaa catgcctttg ggcatctggg
      301 gcatgccgcc gccgccgagg aggccgccgg ccatctgcgc catggcgccc ttgtttttca
      361 tcagcttgcg catcatctcg aactgcttga ggagctggtt gagctccacc acggggcggc
      421 cggcgccttt ggcgatgcgc ttgcgccggc tgccgttgag gatctcgggg cgggcgcgct
      481 ccttgggcgt catggagagg atgatggcct ccacatgcct catgcgcttg tcgtccacgc
      541 cgggcatgcc tttgagcttg cccatgccgg ggatcatgcc gagcaggccc tccagagggc
      601 cgagcttttt catgaagcgg agctggccga ggaagtcgtt gaagtcgaat ttgttctgct
      661 ccaggcggcg catcatgctc atggcctcct tctcgtctat ttcctgggcg gctttttcga
      721 cgaggctgac cacgtccccc atgccgagga tgcgctgcgc catgcggtcg gggtggaaga
      781 cctcaagctg gtcggtcttt tccccgacgc cgatgaactt cacggggcag ccggtgacct
      841 ggcgcatgga gagcagcgcg ccgccgcggg cgtcgccgtc gagcttggac atgatgaggc
      901 cggtgatgcc gatggcttca tggaacttgg aggcgacgct gacggcctgc tggccggtgg
      961 cggcatcggc cacgagcagg gtctcgcgcg gctgcaccag atcacgcacg gccttcaact
     1021 cggcgagcaa ggcctcgtcc acctcctggc ggccggcggt gtcgaagatg gcgatgccgg
     1081 cgccctgctg gtcgagccag gcgagggcgg ctttggcgga cctcaccaca tccgtctcgc
     1141 cggcggcggg ggcgaagacg ggcgcgccga gctgctggcc gagcacctgg agctgggtga
     1201 tggcggcggg gcggtaaagg tccagggcga cgaggagggg gcgcctgccc tgctttttga
     1261 gccaggcggc gagcttggcg gaggtggtgg ttttgcccgc gccgttgagg ccggcgagca
     1321 ggatgcgctg cggcggggag agatcgagtt cctgggcgcc gccgccgagc agcttgacaa
     1381 gctcgtcgtg gaagattttg acgatctgct ggcctgggga gacattgcgc agcacctcct
     1441 cgccaagggc gcgggccttg acggactcga cgaggtcttt ggcgaccttg aagtccacgt
     1501 cggcctcaag gagggcgagg cggatttcac gcagcgcgtc ggccacgttg gactcgctga
     1561 ttttgccgag gccgcgcagc ttgcggaagg cgtcttcgag tttgtcggtg agggcggaaa
     1621 acatgggcgc attctttagc cccggatgcc tgcgaagcaa ggagcgtgtg ggcttgcttt
     1681 caggcgcgga gggcggagac tggcgctcat gaaggccaac ccccgctttc tcctcacggc
     1741 cctctttgcc gcaactgcct tgtccttgca gggcactgac ttgtactcca tcggccagcc
     1801 gactgacgag gagcagttct acctggagct catcaaccgc gcccgcgcga atcccgccgc
     1861 cgaaggcgcg cgcctggcgg cggcgacgga cccggacgtg ctcagcgcca tcgccacttt
     1921 caatgtgaac ctgacggtga tgcagaacga gttcaacgcc ctgccggtgc ggccgccgat
     1981 ggcgttcaac ctcaagctca tgctggccgc gcgcgggcac agccaggaca tgttcaacaa
     2041 tgtcttccag gggcacgacg gcagcaacgg ctccacgctc tcccagcgtg tgaccgccca
     2101 gggctatgcg ttttccagcc tgggggagaa tgtgttctcg ttcgcctcct cggtcttcca
     2161 cggacacgcg ggcttccaga ttgactgggg cgaggagggc gacggcaccg gcatgcagat
     2221 cggccgcggc cacaggacca gcattcactc ggcccggcgc gaggtgggca tcggcatggt
     2281 gctcggcagc aagacgcagg gcgcgaccac ggtggggcca caactggtga cacaggattt
     2341 cggcaccggc aatccggaca accaagcctt cgtgaccgga gtggcgtttt acgatctcaa
     2401 tggcaacagc ttctacgacc ccggcgaggg cgtcggcggt ctgacggtga atgtggcggg
     2461 ctccgccttc cacgccgtca cggccaactc cggcggctac accgtgccgg tgccgacgac
     2521 gaacaccacg cgcgcggtca ccttcgccgg cctcggcctc aatggcggcg cggacgccgt
     2581 gatcagcggc ggcgcgaatg tgaaggtgga cttcgcgccc gcgtattcgc cgccggtgtt
     2641 gtcggggccg gtggacatcg ccctcaacga ggcggccacg cattccttca ccacggtggg
     2701 cggcgccacg ggttacgagc tgcgcgccgt gcccgacgcg cccgtgccgg tggatggcgc
     2761 ggagaatctg ggcctggtca ccgccgctac caccggcgcc tacacgccgc gctccaccac
     2821 ggtcaagcac gcgggcacct ccgcctaccg gctgacgcat ccggcggtgg cggtgagcaa
     2881 ggagcgtctg acttacaatg cggcttacct ggtgcaaagc ggcggcgctt tgtccttccg
     2941 cagccggctg cgcctggcct cggcggacca gaccgcgcag gtgcaggtat ccacggacgg
     3001 cggcttcacc tggcagacgg tcttcagcca gtcgggcact ggcggcgcgg gcgagggttc
     3061 gtttcaactg cgcaacattt ccctggccgc ctttgagggg agggaaatcc gcgtccgctt
     3121 cgaatacgcc ttccccggcg gcagcc
//
LOCUS       DAOMYJ010000385         3117 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Prosthecobacter sp. isolate AMR_MDS_5629
            NODE_11461_length_3117_cov_18.584585, whole genome shotgun
            sequence.
ACCESSION   DAOMYJ010000385 DAOMYJ010000000
VERSION     DAOMYJ010000385.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867850
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prosthecobacter sp. (wastewater metagenome)
  ORGANISM  Prosthecobacter sp.
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Verrucomicrobiaceae; Prosthecobacter.
REFERENCE   1  (bases 1 to 3117)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3117)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.30
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:34:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,742
            CDSs (total)                      :: 4,684
            Genes (coding)                    :: 4,676
            CDSs (with protein)               :: 4,676
            Genes (RNA)                       :: 58
            tRNAs                             :: 55
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3117
                     /organism="Prosthecobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11461_length_3117_cov_18.584585"
                     /isolate="AMR_MDS_5629"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1965333"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            164..1480
                     /locus_tag="PLP58_22080"
     CDS             164..1480
                     /locus_tag="PLP58_22080"
                     /inference="COORDINATES: protein motif:HMM:NF021446.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2157 domain-containing protein"
                     /protein_id="HRJ11286.1"
                     /translation="MSASRNRWLLEQLPLWEGEGLLTAEAARTLRARHAVEAAGPGLG
                     QIMMGALGALLLGSGLIAVIGHNWDEFSRPVRLLFAFLPLLLAQLFSWRVLRHGAAAA
                     WVRETAGLLQALTAGACIALVSQIYNLGGEWTDFLFWWFMLSLPLAWVLMADSVVVFY
                     AAATAVWALSRNETGIPWHDSPLIYPLLLLALLPRWWRRPAPSGLMRWILALSAVTGL
                     CSAAAFTVGHGGVFRSSFSAVVLLWSFTAACLVLLPLNARGIAESAARKPQVVLGCLW
                     LFGYGVGATFPSACRELLKGVEKAVAAPWGQGLLAVLAVFTVLALIQRRWAVLAIASV
                     VALPLLARLPRAEAEGTGGTLLSGLFTAHLAVIGLTLILLDFAGKRAAPRLGAALLSV
                     LVLARMADSHFSLLAKGLAFMGVGAAFLAFNFFMSRRARRQREGAA"
     gene            1477..2049
                     /locus_tag="PLP58_22085"
     CDS             1477..2049
                     /locus_tag="PLP58_22085"
                     /inference="COORDINATES: protein motif:HMM:NF025703.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDYXXLXY domain-containing protein"
                     /protein_id="HRJ11287.1"
                     /translation="MRWPPFVIFALAVLAQWAVPLAGIWRHEQVLARGALIKLKCTAP
                     DPYDPLRGRYLVVLPEQSEVPVDKGMEDHRGAAFAVLEVDADGFAKVIRVERAAPASG
                     LFVKVRIRYAYGGKARFDWPFDRFYLNEKLAPEADKWLAESLRGAKGVTAEVRVLDGR
                     AVLFDLSLGGRSFREHLEERLEGGKTPGQD"
     gene            complement(2069..3058)
                     /locus_tag="PLP58_22090"
     CDS             complement(2069..3058)
                     /locus_tag="PLP58_22090"
                     /inference="COORDINATES: protein motif:HMM:NF012485.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="secretin N-terminal domain-containing protein"
                     /protein_id="HRJ11288.1"
                     /translation="MSFVVGKTLLIGDPSTSSLIVSGSPEHIAIIDQLIEEMDVRPQQ
                     VYISTIIGQLSLGGEFNYGFDFLRLLDDFTLRQKGVAAPAGTTSVAGALDIPFNMDGI
                     STASLGLYGQLGSLSRYVNLLEGNRNFKVLQSPSMYTINNGKAVISSGQRIAVPVNIL
                     SNAGFNNVAATSASIDYRDVVLKLEVIPMINSDNEVTLRIAQVNDNIVGQQDISGNTV
                     PTIGTQELLTTVTVKDGATVVLGGLITERVSNNERGGIFLRRVPVLKHLFGTTQKENL
                     RDELLIFIQPKIINAKDALDSPNQLEAGRSSILDETIRFGMPIEQIPRALPAK"
BASE COUNT          409 a          857 c         1236 g          615 t
ORIGIN      
        1 ggaggcgggt ggctgcgggg gctgacgggt gtggcggcgc gccaccgggg cgcaggccgg
       61 cctggctgct gtccggcaag agggatttcc gtggccgcgc ggcggcggca ggaattattt
      121 gcttgcggcg gcggcgggct tctggcggaa tgcgcggaaa ttcatgagcg cctcacgcaa
      181 ccgatggctt ctggagcaac tcccgctgtg ggaaggggag gggcttttga cggcggaggc
      241 ggcgcggacg ctgcgggcca ggcacgcggt ggaggcggcg gggcccggcc ttggccagat
      301 catgatgggc gcgctgggcg cgctgctcct ggggtcgggg ctgatcgcag tcatcgggca
      361 taactgggat gaattttcgc ggcctgtgcg gctgctgttc gcttttctgc cgctgctgct
      421 ggcgcagctt ttcagttggc gggtgctgcg gcacggcgcg gccgcggcct gggtgcggga
      481 gacggcgggg cttttgcagg cgctgacggc tggggcctgc atcgcgctgg tgtcgcagat
      541 ttacaatctg ggcggggagt ggacggattt tctgttctgg tggttcatgc tgagcctgcc
      601 gctggcgtgg gtgctgatgg cggattcggt ggtggtgttt tacgccgccg ccaccgcggt
      661 gtgggcgttg agcaggaatg agacgggcat cccgtggcat gacagcccgc tgatttatcc
      721 gctgttgctg ctggcgctgc tgccgcgctg gtggcggcgt cccgcgccct cggggctgat
      781 gcggtggatc ctggctttga gcgcggtcac cgggctgtgc tcggcggcgg ctttcacggt
      841 ggggcacggg ggcgttttca gatcgagctt cagcgccgtc gtgctgctct ggtccttcac
      901 ggcggcctgc ctggtgctgc tgccgctcaa tgcgcgcggc atcgcggaat ccgcggcgcg
      961 caagccgcag gtggtgctgg gctgcctgtg gctgtttggc tacggcgtgg gcgccacttt
     1021 cccgtctgct tgcagggagt tgctcaaggg ggtggagaag gccgtggccg cgccctgggg
     1081 acagggcctg ctggcggtgc tggcggtctt cacggtcctg gcgttgatcc agaggcgctg
     1141 ggcggtgctg gcgatcgcct cggtggtggc gctgccgctg ctggcgcgcc tgccgcgcgc
     1201 ggaggccgaa gggacgggcg gcacgctgct ctcggggctt ttcacggcgc acctggccgt
     1261 gatcggactg acgctgatcc tgctggactt cgccggcaaa cgcgccgcgc cgcgccttgg
     1321 cgccgcgctg ctcagcgtgc tggtgctggc tcgcatggcg gacagtcatt tctccctgct
     1381 ggcgaagggc ctggccttca tgggcgtggg cgcggctttt ctggccttca actttttcat
     1441 gagccgccgc gcgcggcggc aaagggaggg cgcggcatga ggtggccgcc ctttgtcatc
     1501 ttcgcgctgg ccgtgctggc ccagtgggcg gtgccgctgg ccggaatctg gcggcatgag
     1561 caggtgctgg cgcgtggcgc gctcatcaag ctcaaatgca cggctcctga tccatacgat
     1621 ccactgcgcg ggcgctacct cgtggtgctg cccgaacagt cggaggttcc cgtggacaag
     1681 gggatggagg atcatcgcgg cgcggccttt gccgtgctgg aggtggacgc cgacggtttc
     1741 gccaaagtca tcagggtgga gcgcgcggcg cccgcatccg ggctgttcgt gaaggtcagg
     1801 attcgttatg cctatggggg caaggcgcgc tttgactggc cgttcgaccg cttttacctc
     1861 aatgagaaac tcgcgcccga ggcggacaaa tggctggccg agtccctgcg cggcgccaag
     1921 ggagtgaccg ccgaggtccg ggtgctggac gggcgcgcgg tgctttttga tctgagcctg
     1981 ggcggcagat ctttccgcga gcatctggag gagcgcctgg agggcggcaa gacgccgggt
     2041 caggactgag ctccgggggc gcgggcgctc acttcgcggg cagggcgcgg gggatttgct
     2101 cgatgggcat gccgaagcgg atggtctcat cgaggatgct ggagcgtccg gcctcgagct
     2161 ggttggggga gtcgagggcg tccttggcgt tgatgatctt cggctggatg aagatgagca
     2221 gctcgtcgcg caggttctct ttttgcgtgg tgccgaaaag gtgcttcaag acggggacgc
     2281 ggcgcaggaa gatgccgccg cgctcgttgt tggagacgcg ctcggtgatg aggccgccga
     2341 gcacgacggt ggcgccgtcc ttgacggtga cggtggtcag cagctcctgg gtgccgatgg
     2401 tgggcacggt gttgccggag atgtcctgct ggccgacgat gttgtcattg acctgggcga
     2461 tgcggagggt gacctcgttg tcggagttga tcatggggat gacttcgagc ttcaggacga
     2521 catcccggta gtctatgctg gcgctggtgg cggcgacgtt gttgaagccg gcgttggaga
     2581 ggatgttcac gggcacggcg atgcgctggc cggaggagat gacggccttg ccgttgttga
     2641 tggtgtacat gctggggctt tgcaggactt tgaagttccg gttgccctcc aggaggttca
     2701 cataacggct gaggctgccg agctggccgt aaaggccgag gctggcggtg ctgatgccgt
     2761 ccatgttgaa cggaatgtcg agcgcgccgg caacgctggt ggtgccggcg ggcgcggcca
     2821 cgcctttttg ccggagggtg aagtcgtcga gcagccgcag aaagtcgaag ccgtagttga
     2881 actcgccgcc gaggctgagc tggccgatga tggtggagat gtacacctgc tgcgggcgca
     2941 cgtccatttc ctcaatgagc tggtctatga tggcgatgtg ctcgggggag ccgctgacga
     3001 tgaggctgct ggtggagggg tcgccgatga ggagggtctt gccgacgacg aaggacatgg
     3061 gcgccgcaag gtgacggaaa gtgcaacaga aaacaaaccg ccgatgtccc ggctcag
//
LOCUS       DAOMYJ010000386         3095 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Prosthecobacter sp. isolate AMR_MDS_5629
            NODE_11572_length_3095_cov_14.103947, whole genome shotgun
            sequence.
ACCESSION   DAOMYJ010000386 DAOMYJ010000000
VERSION     DAOMYJ010000386.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867850
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prosthecobacter sp. (wastewater metagenome)
  ORGANISM  Prosthecobacter sp.
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Verrucomicrobiaceae; Prosthecobacter.
REFERENCE   1  (bases 1 to 3095)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3095)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.30
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:34:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,742
            CDSs (total)                      :: 4,684
            Genes (coding)                    :: 4,676
            CDSs (with protein)               :: 4,676
            Genes (RNA)                       :: 58
            tRNAs                             :: 55
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3095
                     /organism="Prosthecobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11572_length_3095_cov_14.103947"
                     /isolate="AMR_MDS_5629"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1965333"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            698..770
                     /locus_tag="PLP58_22095"
     tRNA            698..770
                     /locus_tag="PLP58_22095"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:731..733,aa:Phe,seq:gaa)
     gene            905..1777
                     /locus_tag="PLP58_22100"
     CDS             905..1777
                     /locus_tag="PLP58_22100"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015411.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HRJ11289.1"
                     /translation="MIKTLTGQKIGFAGLGNMGSAMARHLHEAGAEMVVWNRSDAPAE
                     KAATLGMRRALSLTELAREIGAGIVCINLTTTEVVEQMVFGAGGLAEGLSPEALIIDF
                     GTTSVPATREFARRARWLDVPVSGGQVGAEAGNLTLMAGGSAEDFARALPVLRVVGER
                     ITHMGGPGAGQVTKLANQLIVAQTIDAVAQALRLAELAGVDAALVREALLGGFAESRI
                     LHLHGDRMVRRDFAPGGRSTLQLKDVRLICELAGSLGFHSPTLENCRAQWETFVHEKG
                     GGDLDHSGLFRMYE"
     gene            complement(2138..>3095)
                     /locus_tag="PLP58_22105"
     CDS             complement(2138..>3095)
                     /locus_tag="PLP58_22105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009958323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="carboxypeptidase M32"
                     /protein_id="HRJ11290.1"
                     /translation="SLRSSSVDVASLCRSAQAPAALPRGRYPVAKQQQLNTEIAASIG
                     FDFDCGRIDTTAHPFCTTLGPRDVRLTTRYDEADFTSSLFGVMHEAGHGLYEQGLPGD
                     DFGLPSGSACSLGIHESQSRLWENHVGRSRPFWEKWLPRAQEIFPHLKKTSLESFLAF
                     IHRARYSFIRVEADEATYDLHILLRFSIERRIVSGELPVEKVPEAWNDEFHQLFGVFP
                     PDDARGCLQDIHWSMGGLGYFATYTLGNLNAAQLFATALRQRKTAAAMDRADYAPLLA
                     WLREKVHAQGGAGLPGEVMTRATGRPTEAAWHLKHLRARFLV"
BASE COUNT          556 a          953 c         1079 g          507 t
ORIGIN      
        1 ccggtccagg cgggcggact ttgcgcggga ggaaaaagcg gacccgcgcc ctgctgtggc
       61 aagccctgcc cgggagcggc accgccaaga agagcctggc caagaccggg cggctgccaa
      121 gcgggaggag attgcggaag cggactttga gccgatgagg gcgcaggccc cgggccgccc
      181 tgccatgctg gagcacccgc gcccaaggga ttcagatgcc gggccagcga ggccggatca
      241 ggcagcactg gacctgcggc agtcgcggct gacggcgact gcgcgggcgg tgcgggtgga
      301 aacatgggcg ctgccgctgc ttgaggtgag gtcacagtct gaccacacac agggcaagga
      361 cccgacactc cggcgagata ccccggcacc gtgagggtgc tttggcagga agaacattga
      421 aactggatcg tgtcaggagc catgaggggg atttcaatac ccgcaggtat ttttttaaat
      481 ttcgctcaac tcaccagttc gacaagcacc attctgatga ttataaagcc ccgacatcct
      541 ttgcgcggcc tgcatcaaga cgctcatccg ccatttgtcg cccacgacgc caccacgcat
      601 ggtctgctct gcatgaagaa acaaaatgca aaaagccgtt gcacgctgct gactgtcttg
      661 ccacaatcgc ggcccgctga aaacagcgga aagtgaaggc tcggtagctc agtcggtaga
      721 gcaggggatt gaaaatcccc gtgtcggcgg ttcgattccg tcccgagcca cctcttaaga
      781 cgccaaagca tggcgcggcc gctcaaacca ccaaaaacac gacacctgcg cacggcgccc
      841 acccctgccc cgcgttcccc gaaactttct ttgcattccc gccgccgcgc tctcgaatga
      901 gtggatgatc aagactttga ccggacaaaa aatcggcttc gcgggattgg gcaacatggg
      961 atcggccatg gcgcggcatt tgcatgaggc gggggctgaa atggtggtgt ggaaccgcag
     1021 tgacgcgccc gctgaaaaag ccgccacgct gggcatgagg cgcgcgcttt ccctgacgga
     1081 gctggcgcgc gagattggcg cgggcatcgt ctgcatcaat ctcaccacta ccgaggtggt
     1141 ggagcagatg gtcttcggcg cgggcgggct ggcggaggga ctttcgcccg aggcgctcat
     1201 catagacttc ggcaccacgt ccgtgccggc cacgcgcgag ttcgcccggc gcgcgcgctg
     1261 gctggatgtg cccgtctccg gcggccaggt gggcgcggag gcgggaaacc tcaccctcat
     1321 ggccggcggc agcgcggagg acttcgcgcg cgcccttccc gtgctgcggg tggtgggcga
     1381 gcgcatcacc cacatgggcg gcccgggcgc cgggcaggtc acaaagctgg ccaatcaact
     1441 catcgtggcg cagaccattg acgctgtggc gcaggcgctg cggctggccg agctggcggg
     1501 cgtggatgca gcgctcgtgc gcgaggcgct gctaggcggc ttcgccgaaa gccgcatcct
     1561 gcatttgcac ggggaccgca tggtgcggcg cgacttcgct cccggcggac gctccaccct
     1621 ccagctcaag gacgtgcgcc tcatctgcga gctggccgga agcctcggct tccactcccc
     1681 cacgctcgaa aactgccgcg cgcagtggga gaccttcgtc catgaaaaag gcggcggcga
     1741 cctggaccac tccggccttt tccgcatgta tgagtgagct gtggtcgccg ccgcaaggcg
     1801 gcgcatcagc gtggcggcca atccaagcac caccaccttg tcacagcact ctcactgcga
     1861 aagcgccgaa cagcccggac tctccctgcg gacctcccgc tcgcgggatc gggatcacgc
     1921 agccttccgc aaccacgctt ggcgtcggca gtgtggatga tgatcttgag gggactcccg
     1981 aaaactctga gcatgggcgc aggcatcctg cctgcgcgaa caggcgggac gcctgttccc
     2041 catgcaaaag cgggttttcg gaagtaccct tgatttgctc tcatcccgtt tcgcgcaaat
     2101 cggaatcgca accggctcac tcccgcgttt cccggtttca gactaaaaag cgcgcgcgca
     2161 ggtgcttcag atgccaggcg gcttcggtgg ggcggccggt ggcgcgggtc atgacttcgc
     2221 cgggcaggcc ggcgccgccc tgggcgtgga ctttttcacg cagccaggcg agcagggggg
     2281 cgtagtcggc gcggtccatg gcggcggcgg tcttccgctg cctgagtgcg gtggcgaaga
     2341 gctgggcggc attcaggttc cccagcgtat aggtggcgaa gtagccgagg ccgcccatgc
     2401 tccagtggat gtcctgcagg cagccgcgcg cgtcatccgg cgggaacacg ccgaagagct
     2461 ggtggaactc gtcattccag gcttcgggga ctttctccac aggcagctcg ccgctgacga
     2521 tgcggcgctc gatgctgaag cggaggagaa tgtggaggtc gtaggtggcc tcgtcggcct
     2581 cgacgcggat gaaggagtaa cgggcgcggt gaatgaaggc gaggaagctc tccaggctgg
     2641 tttttttcag atgcgggaag atctcctgcg cgcgcggcag ccatttttcc cagaaggggc
     2701 gggagcggcc gacgtggttc tcccacaggc ggctctggga ctcgtggatg ccgagggagc
     2761 aggcgctgcc tgagggcagg ccgaagtcgt cgccgggcag cccctgctcg taaagaccgt
     2821 gaccggcctc gtgcatgacg ccaaagaggg aggaggtgaa gtcggcctcg tcatagcggg
     2881 tggtgaggcg cacatcgcgc gggccgaggg tggtgcaaaa ggggtgcgcg gtggtgtcta
     2941 tgcggccgca gtcgaagtcg aagccgatgc tggcggcaat ctcggtgttg agctgctgct
     3001 gtttggcgac ggggtagcgc ccgcgcggga gggcggcggg ggcctgggcg ctgcggcaca
     3061 aggatgcgac gtcgacgctg gaggacctga gcgaa
//
LOCUS       DAOMYJ010000387         3093 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Prosthecobacter sp. isolate AMR_MDS_5629
            NODE_11581_length_3093_cov_20.736669, whole genome shotgun
            sequence.
ACCESSION   DAOMYJ010000387 DAOMYJ010000000
VERSION     DAOMYJ010000387.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867850
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prosthecobacter sp. (wastewater metagenome)
  ORGANISM  Prosthecobacter sp.
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Verrucomicrobiaceae; Prosthecobacter.
REFERENCE   1  (bases 1 to 3093)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3093)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.30
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:34:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,742
            CDSs (total)                      :: 4,684
            Genes (coding)                    :: 4,676
            CDSs (with protein)               :: 4,676
            Genes (RNA)                       :: 58
            tRNAs                             :: 55
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3093
                     /organism="Prosthecobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11581_length_3093_cov_20.736669"
                     /isolate="AMR_MDS_5629"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1965333"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..355)
                     /locus_tag="PLP58_22110"
     CDS             complement(<1..355)
                     /locus_tag="PLP58_22110"
                     /inference="COORDINATES: protein motif:HMM:NF017534.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HRJ11291.1"
                     /translation="MTDWDQCYITGQTPWDKGAPSPPLLAWVERSRPQGRALVPGCGI
                     GHDVALLAASGMDATGLDVAPTAVERARLAYPELAERFVLGDLFATPPEWDGAFDWII
                     EHTCLCALPPARRADY"
     gene            complement(410..3034)
                     /locus_tag="PLP58_22115"
     CDS             complement(410..3034)
                     /locus_tag="PLP58_22115"
                     /inference="COORDINATES: protein motif:HMM:NF014585.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase B/oxoprolinase family protein"
                     /protein_id="HRJ11292.1"
                     /translation="MARIDGRPGWRFTQEVAGMQLLAPCVAIETVAAGGGSICRWTPQ
                     GLTVGPESAGADPGPACYGKGGPLTITDVNLLLGRFDPARAPIPLDAEAARRRLLELH
                     AESDGSLAPQELLRACLRLAVEHMADAIRRISVAKGYDPRDYALLAFGGAGPQHACAV
                     AEHLGMSTILAPQHAGILSAVGLHEAVREKFAARQILRPLAECAGDLEDWLRTLTDEA
                     ASALGHPAETSAAFLAEMRLRGQDTPLQVEFENAADLEALYRAAYQRLFGYAPPAARL
                     IELVSLRVVARATGGEKSPPLTCEPEALGAGPLLIQDEFSTLVVEPGWQAALLPGFGY
                     VLTWAVGVQALACSGKGSATESTPVSPKGFTPTRDLIRHRLHSIVSEMGALLCRTAIS
                     TNIRERLDFSCALLDAEGRLVSSAPHIPVHLGALGVCVREAVRGFDLQPGDTLITNHP
                     AFGGSHLPDVTLITPVFEAGGGALLGYVANRAHHAEIGGITPGSMPATATCLEQEGVV
                     IAPRFLVKAGESCFEEIAALLTGAAHPTRNVADNLADLHAQLAANQLGVERLRALAGA
                     DGEGLREALQGILEHSAEVMRAFLPQARAGMAEQALDDGSLLKIQVSHVNEVTPVKTL
                     RLDFTGTAPTHQGNLNATRAIVRSVVLYCLRLLLREVLPLNEGLLEPVEVVLPEGTLL
                     NPVFTGDAARDPAVVGGNVEISQRLVDTLLLAFGLQACSQGTMNNFLFGDGRFGYYET
                     VAGGTGAGPGYEGAHALHSHMTNTAITDPEVLEQRYPVRLRQFAIRQGSGGAGRWRGG
                     DGVVREFEFLAPLTLSLLTQHRSSGPYGMEGGGAGAVGRQILIRDGKEKELPASASLP
                     VLPGDRVRLETPGGGGWG"
BASE COUNT          524 a         1149 c          974 g          446 t
ORIGIN      
        1 cgtagtccgc gcgccgcgcg ggcggcagcg cgcacaggca ggtatgctcg atgatccagt
       61 caaacgcgcc atcccactcc ggcggagtgg cgaaaaggtc cccgagcacg aaacgctccg
      121 ccagctccgg gtaagccagg cgcgcgcgct caacagcggt cggagccacg tccagacccg
      181 tggcatccat gcccgaggcc gccagcagcg ccacatcatg cccgatgccg cagccgggca
      241 ccagcgcccg tccctgcgga cggctccgct ccacccacgc gagcagtggt ggcgagggcg
      301 cgcccttgtc ccagggcgtt tggccggtga tgtagcattg gtcccagtcg gtcatcgggc
      361 cgcgaggtta gggcgcgttt gaagcctggc aagcacgctt caaaaaccct catccccagc
      421 cgccgccacc gggcgtctcc agccgcacgc ggtctcccgg cagcaccggc aggctggcgc
      481 tggcaggcag ctctttttcc ttcccatccc ggatcaaaat ctgccgcccc actgcccccg
      541 cgccgccgcc ctccatgcca tacgggccgc tgctccggtg ctgggtgagc aaggacaaag
      601 tcagcggcgc caggaactcg aactcccgca ccacgccatc cccgccgcgc cagcgccccg
      661 cgccgccgct gccttggcga atggcgaact gcctcagccg caccgggtag cgctgctcca
      721 gcacctccgg gtcggtgatg gccgtgttcg tcatgtggct gtgcaaagcg tgcgcgccct
      781 cgtaacccgg ccccgcgccg gtgccgccag ccactgtctc gtaatagcca aagcggccgt
      841 cgccgaacag aaagttgttc atcgtgccct ggctgcacgc ctggaggcca aaggccagca
      901 gcagcgtgtc caccagccgc tggctgatct ccacattgcc gcccaccacg gcggggtcgc
      961 gcgcggcatc tccagtaaag acgggattca aaagcgtgcc ctccggcaat accacctcca
     1021 ccggctccag cagcccctca ttgagcggca gaacctcccg cagcagcagg cgcaggcagt
     1081 agagcaccac actgcgcacg atggcgcgcg tggcgttcag attcccctga tgcgtaggcg
     1141 cggtgcctgt gaagtcgagg cggagcgtct tgactggagt gacttcattg acgtggctga
     1201 cctggatttt taggagcgag ccgtcatcca aagcctgctc cgccatgccc gcgcgggcct
     1261 gcggcaggaa ggcgcgcatg acctccgcgc tgtgctccag gatgccctgc agagcctcgc
     1321 gcagcccctc gccatccgcg ccagccagcg cgcggaggcg ctccacaccg agctgattgg
     1381 ccgcgagctg cgcatgcagg tcggccaagt tatccgccac attccgcgtg ggatgcgcgg
     1441 ctccggtgag cagcgcggcg atttcctcaa aacacgactc ccccgccttc accaaaaatc
     1501 gcggcgcgat gaccacgccc tcctgctcca ggcacgtggc cgtggccggc atggagccgg
     1561 gtgtgatgcc gccaatctcc gcatgatgcg cgcggtttgc cacatagccc agcagcgcgc
     1621 cgccgcccgc ctcgaacacc ggcgtgatga gtgtgacatc cggcaggtgc gagccgccga
     1681 atgccgggtg gttcgtgatc agcgtgtccc ccggctggag gtcaaagcca cgcaccgcct
     1741 cccgcacgca cacgccgagc gcgcccagat gcaccgggat gtgcggcgcg ctggagacca
     1801 gccgcccttc cgcgtccagc agcgcgcagg aaaagtccag ccgctcgcga atgttcgtcg
     1861 agatggccgt gcggcaaagc agcgcgccca tttcggagac gatgctgtgc agcctgtggc
     1921 ggatgagatc gcgcgttgga gtgaagcctt taggcgacac gggagtggac tccgttgcag
     1981 agcctttccc ggaacaagct aaagcttgaa ctccaacagc ccacgtcagc acatacccga
     2041 agcccggcag cagcgccgcc tgccaccccg gctccaccac cagcgtgctg aattcatcct
     2101 ggattaaaag cggccccgcg cccagcgcct ccggttcaca ggtcaaaggc gggctttttt
     2161 ccccgcccgt ggcacgcgcc accacgcgca ggctgaccag ctcgatgaga cgcgcggcag
     2221 gcggcgcata accaaaaaga cgctgataag cggcgcgata cagcgcctca agatcagcgg
     2281 cgttttcgaa ctccacctgc aaaggcgtgt cctgcccgcg cagacgcatc tctgccagaa
     2341 atgccgcgct tgtctccgcg ggatgcccca gcgcagaggc ggcctcatcc gtcagagtgc
     2401 gcagccaatc ctccaaatcc cccgcgcact ccgccagcgg gcgcaggatt tgccgcgcgg
     2461 cgaatttttc ccgcaccgcc tcgtgcagtc ccaccgcgct caggatgccc gcgtgctgcg
     2521 gtgccaggat ggtgctcatg cccaggtgct ctgccaccgc gcaggcgtgc tgcggccccg
     2581 ccccgccaaa ggccagcagc gcgtagtcgc gcggatcata ccccttggcc accgagatgc
     2641 ggcggatggc atccgccatg tgctccacgg ccaggcgcag gcaggcgcgc agcagttcct
     2701 ggggggcgag gcttccgtcc gactccgcgt gcagctccag caggcggcgg cgcgcagctt
     2761 cggcatcgag cgggatcggc gcgcgcgcgg gatcgaagcg gccgagcagc aaattcacat
     2821 ccgtgatggt cagcggtccg cctttgccat aacaggccgg gccggggtcg gcgcccgcgc
     2881 tctccggccc cacagtcagg ccctgcggcg tccagcggca gatggagccg ccgcccgccg
     2941 ccacggtctc gatggccacg cacggagcca gcaattgcat cccggccacc tcctgcgtga
     3001 agcgccagcc gggccgcccg tcaatgcgcg ccacatccgt gctcgaccgc ctgcgtactg
     3061 tccggcgccg cagccgggat catcgccaag agg
//
LOCUS       DAOMYJ010000388         3083 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Prosthecobacter sp. isolate AMR_MDS_5629
            NODE_11624_length_3083_cov_20.502642, whole genome shotgun
            sequence.
ACCESSION   DAOMYJ010000388 DAOMYJ010000000
VERSION     DAOMYJ010000388.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867850
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prosthecobacter sp. (wastewater metagenome)
  ORGANISM  Prosthecobacter sp.
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Verrucomicrobiaceae; Prosthecobacter.
REFERENCE   1  (bases 1 to 3083)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3083)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.30
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:34:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,742
            CDSs (total)                      :: 4,684
            Genes (coding)                    :: 4,676
            CDSs (with protein)               :: 4,676
            Genes (RNA)                       :: 58
            tRNAs                             :: 55
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3083
                     /organism="Prosthecobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11624_length_3083_cov_20.502642"
                     /isolate="AMR_MDS_5629"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1965333"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..286
                     /locus_tag="PLP58_22120"
     CDS             <1..286
                     /locus_tag="PLP58_22120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ11293.1"
                     /translation="EAHLDAGARALLADPALLNSDGSLNLARPLLRLHPALLGHTLRL
                     WLCGQLGLDGIGSAEVGAASSMLAPGGPAKVNLPGGRHLRRKARRLWVEA"
     gene            complement(326..2341)
                     /locus_tag="PLP58_22125"
     CDS             complement(326..2341)
                     /locus_tag="PLP58_22125"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009962004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HRJ11294.1"
                     /translation="MPREYTLHTHAPAGPRIDYAAELNEQQHAAVTAPPGQTLIIAGA
                     GSGKTRTLTYRVAWLLDNGVLPEQILLLTFTNKAAREMLERVTALVPVETQRLWGGTF
                     HSIGNRLLRWNAERLGYRKGFSIMDREDQKDLMETVVAASGVDTSGFRFPKAEVLGDI
                     YSMADNTCEPLEEVLAVRYPWFEQVAPHIIELRRLYQEKKRETNCMDFDDLLTLSVKL
                     LEENEDLLERYRRQFEFILVDEYQDTNSLQCRFVEMLTGPDGNLMVVGDDAQSIYSWR
                     GADVGNILNFTDHWPGARTHKIEVNYRSVPEVLELANASIANNRAQIRKSLRAARPSQ
                     GNLPALVPLDNASAQAGFVAQRILELADEGVEFNEMAVIYRAHFHSMEIQMELTSRGI
                     PFQITSGLRFFEQAHVKDVAAFMKFAVNRRDEVSFMRMVRLIPGIGGASANKLWTSWL
                     KSEAASAETMTGTFSEVLLPMNVPKKARETWDQLAYTLDELMADGKPAAPPMMIRSIV
                     EGVYDDYMKAKFKNADQRAQDLEQLSNFSDRFTDTLEFLSQLSLLSGVDTSDKPDAQP
                     QDRDAVTLTTAHQAKGLEWHSVFAVWLADGMFPHKRAIEDGGDTALEEERRLFYVTVT
                     RAKDNLHLTYPVLNHAARDGDILMKPSRFITELPQDILEKWNVRGGW"
     gene            2447..>3083
                     /locus_tag="PLP58_22130"
     CDS             2447..>3083
                     /locus_tag="PLP58_22130"
                     /inference="COORDINATES: protein motif:HMM:NF013353.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0036361 - racemase activity, acting on
                     amino acids and derivatives [Evidence IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate/glutamate racemase family protein"
                     /protein_id="HRJ11295.1"
                     /translation="MRAFILLLSLALCCRADDVVERIIAHAAAHPAGDAPVSFERAVF
                     DKDTRHLPIGVFDSGIGGLTVLEALLTLDAFHNDSLQPGADGVPDFARERFIYFGDQA
                     NMPYGNYSAAGRTDYLRELILKDAVFLLGSRWHDGKAARPGKPPVKAIVIACNTATAY
                     GLEDIRAAVKAWGLPVIVIGVVEAGARGVLETGGKTGAVGVLATVGTCASGA"
BASE COUNT          475 a         1029 c          995 g          584 t
ORIGIN      
        1 cgaggcgcat ctcgatgccg gggcgcgcgc cctgctggcc gatcccgcct tgctgaacag
       61 cgacggcagc ctcaacctcg cccgcccgct cctgcgcctg catcccgccc tgctcggcca
      121 caccctgcgc ctctggctgt gcggacagct tggattggat ggcatcggca gcgccgaagt
      181 cggggccgcg tcctccatgc tcgctcccgg cggccccgcc aaagtcaacc tcccaggcgg
      241 gaggcatctc cgccgcaaag cccggcgtct ttgggtggag gcttgaggcg ttgcgcaagt
      301 catgtgcgcg cccggctcaa ccagctcacc agccgccgcg gacgttccat ttttccagga
      361 tatcctgcgg cagctcggtg atgaagcggc tcggtttcat gaggatgtcg ccatcccgcg
      421 cggcgtggtt cagcaccggg taggtgaggt gcaggttgtc cttggcgcgg gtgacggtga
      481 cgtaaaaaag ccgccgctct tcttcaagcg cggtgtctcc gccgtcctcg atggcgcgtt
      541 tgtgcgggaa catgccgtcg gccagccaga cggcgaagac gctgtgccac tccagcccct
      601 tggcctggtg cgcggtggtg agagtcaccg cgtcgcggtc ctggggctgc gcgtccggtt
      661 tgtcactggt gtccacgccg ctgagcaggg agagctggct gagaaactcc agcgtgtcgg
      721 tgaagcggtc gctgaagttg ctgagctgtt ccaggtcctg cgcgcgctgg tcggcgtttt
      781 tgaacttcgc cttcatgtag tcgtcgtaaa cgccctcgac gatgctgcgg atcatcatcg
      841 gcggcgcggc gggttttcca tccgccatca gctcgtccag cgtgtaggcg agctggtccc
      901 aggtctcgcg cgcttttttc ggcacgttca tgggcaggag gacttcggag aaagtgccgg
      961 tcatcgtctc cgcgctggcg gcctcgcttt tcagccagga ggtccagagc ttgttcgcgc
     1021 tcgccccgcc gatgccgggg atgagccgca ccatgcgcat gaagctcacc tcatcccgcc
     1081 ggttcaccgc gaacttcatg aaggcggcca catccttcac atgcgcctgc tcgaaaaagc
     1141 gcaggccgct ggtgatctgg aaggggatgc cgcggctggt cagctccatc tggatttcca
     1201 tgctgtgaaa gtgcgcgcgg tagatgaccg ccatctcgtt gaactccacg ccctcgtccg
     1261 ccagctccag gatgcgctgc gccacaaagc cggcctgagc cgaggcattg tccagcggca
     1321 ccagcgcggg cagattgccc tgcgaagggc gcgccgctcg caggctcttg cggatttggg
     1381 ctcggttgtt ggcgatggag gcgttcgcca gctccagcac ctccggcacg ctgcggtagt
     1441 tcacctcgat cttgtgcgtg cgcgcgcctg gccagtggtc ggtgaagttc aggatgtttc
     1501 ccacatccgc gccgcgccag gagtaaatgg actgggcgtc atcccccacc accatgagat
     1561 tgccatccgg cccggtgagc atctccacaa agcggcactg gaggctgttc gtgtcctggt
     1621 attcatccac caaaatgaac tcaaactgcc gccggtagcg ctccagcagg tcctcatttt
     1681 cctccagcag cttcacggag agcgtgagca ggtcatcgaa gtccatgcag ttcgtctcgc
     1741 gctttttttc ctgataaagc cggcgcaact cgatgatgtg cggcgccacc tgctcgaacc
     1801 atggatagcg gacggccagc acctcctcca gcggctcgca ggtgttgtcg gccatggaat
     1861 aaatatcccc cagcacctcg gccttcggga agcggaagcc ggaggtgtcc accccgctgg
     1921 ccgccaccac ggtctccatg aggtcttttt gatcctcgcg gtccatgatg gagaagccct
     1981 tgcggtagcc gaggcgctcc gcgttccagc gcagcaggcg gttgccgatg gagtgaaagg
     2041 tgccgcccca cagccgctgc gtctccactg ggacaagcgc agtcacgcgc tccagcatct
     2101 cccgcgccgc cttgttggtg aaggtgagca gcaggatctg ctccggcagc acgccattgt
     2161 ccagcagcca ggccacgcgg taagtaagcg tgcgcgtctt gcccgagccc gctcctgcga
     2221 tgatgagcgt ctgccccggc ggagcggtca cagcggcgtg ctgctgctca ttgagctccg
     2281 ccgcgtaatc aatgcgcggg ccggccgggg cgtgggtgtg cagggtgtat tctcggggca
     2341 tgtctcacgc ggcttatgcg ccagggcacg gcccggctca agcctcaaag cgcgcccgcg
     2401 tcatgctccg cattgacggc gggcgcggag cgtgatgcac tgcgccatgc gtgcattcat
     2461 tcttctgctc tccctcgccc tctgctgccg cgccgacgac gttgtggaaa ggatcatcgc
     2521 ccatgccgcc gcgcatccgg cgggggatgc tccggtgtcc tttgaacgcg ccgtgtttga
     2581 caaggacacg cgccatctgc ccatcggggt ctttgattcc ggcatcggcg gtctcacggt
     2641 gttggaggcg ctgctcactc tcgacgcttt tcacaatgac agcctccaac ccggcgcgga
     2701 cggggtgccg gacttcgcca gggagcggtt catttatttc ggcgatcagg ccaacatgcc
     2761 ctacggcaac tactccgcgg ccgggcgcac cgattacctg cgggaactga tcctcaaaga
     2821 cgccgtgttc ctgctcggca gccgctggca tgacggcaag gccgcgcgcc ccggcaagcc
     2881 gcccgtgaaa gccatcgtca tcgcctgcaa cacggccacc gcctacggac tggaggacat
     2941 tcgcgcggcg gtgaaagcct gggggctgcc ggtcatcgtc atcggcgtcg tcgaggccgg
     3001 cgcgcgcggc gtgctggaga ccggcggcaa aacaggcgcg gtgggcgtgc tggccacggt
     3061 cggcacctgc gccagcggcg cct
//
LOCUS       DAOMYJ010000389         3077 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Prosthecobacter sp. isolate AMR_MDS_5629
            NODE_11663_length_3077_cov_13.985109, whole genome shotgun
            sequence.
ACCESSION   DAOMYJ010000389 DAOMYJ010000000
VERSION     DAOMYJ010000389.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867850
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prosthecobacter sp. (wastewater metagenome)
  ORGANISM  Prosthecobacter sp.
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Verrucomicrobiaceae; Prosthecobacter.
REFERENCE   1  (bases 1 to 3077)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3077)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.30
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:34:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,742
            CDSs (total)                      :: 4,684
            Genes (coding)                    :: 4,676
            CDSs (with protein)               :: 4,676
            Genes (RNA)                       :: 58
            tRNAs                             :: 55
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3077
                     /organism="Prosthecobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11663_length_3077_cov_13.985109"
                     /isolate="AMR_MDS_5629"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1965333"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..228)
                     /locus_tag="PLP58_22135"
     CDS             complement(<1..228)
                     /locus_tag="PLP58_22135"
                     /inference="COORDINATES: protein motif:HMM:NF012334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HRJ11296.1"
                     /translation="MNATYDVRGLSVLVMGGTTGLGLAAARALARQGARVVVTSRSEE
                     NVRRVKPCFHGFGAQSGPPTRTLADTPHTYHA"
     gene            350..1660
                     /locus_tag="PLP58_22140"
     CDS             350..1660
                     /locus_tag="PLP58_22140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018970260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase family protein"
                     /protein_id="HRJ11297.1"
                     /translation="MIRVRFAPSPTGYLHVGGARTALFNWLFARHHGGKFILRIEDTD
                     EARNTDAARQAILDGLRWLGLDWDEGPEAGGDHGPYFQSQRKSVYDAALARLEAAGRT
                     YTEENGAVRFKFSRTAITVRDLVCGDVTFAPTEEPDMTLRRPDGSYIFHFVNVVDDAE
                     MGITHVIRGEDHLSNTWKHMDLFAALGKDAPAYAHIPLILNPGGSKMSKRDEGASIQS
                     YVDSGFLPDAVFNYLCLLGWTPRAEGEKLDRATLVSLFDPAGVHSANARFDMHKCAWF
                     NAQYLRELDPAALLAAARPFLEMAGIIVTDDSLAAAALQSVKEKVSRLAEFPEWVHYF
                     FREDYPTEEDALAKLRAKPENPALLQAASASFQALTEWTEPAVHTAIEDAAKGAGVKP
                     GALMPLLRFALSGQTRGPGVATIAHLIGKERTLGRIQSALARMG"
     gene            complement(1694..>3077)
                     /locus_tag="PLP58_22145"
     CDS             complement(1694..>3077)
                     /locus_tag="PLP58_22145"
                     /inference="COORDINATES: protein motif:HMM:NF019040.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF1501 domain-containing protein"
                     /protein_id="HRJ11298.1"
                     /translation="CRRRQQALHHECAQRRRLHADGAHRPGVHAPKAKRVIWLFMRGG
                     VSHMESFDPKPALNRHAGKSIGETPFASVQDPEKLKKVRVVVVNDANGQQRNVIYPLQ
                     TGFKKYGRCGVEISDWFPHIGSCADEIAFIRGMWTTDDNHGAQVQFHSGRHMLEPRAP
                     TLGAWVNWGLGSMTDNLPSFIGMGPRYFDTRDGHYLGPAHDAVKLSVDPKNPLAFAVS
                     EGGASAREQAAQFQLIQRLNAITARQHPGDAMLAARMRSYTLAGNMQTSVPETLDLDS
                     ESEETKRLYGLDHKVTEPFARQLLTARRLAERGVRFIQIMHGDGAAGAWDSHSGLKKN
                     HSALAEQVDKPVSGLLKDLKQRGLLDDTLVVFGTEFGRTPGSQGTDGRDHHPYGFSVW
                     MAGGGVKGGVIHGATDELGFHAVEHPHYVTDIHATVLHLLGLNPHRMEIPGRKRLERD
                     FGKVIREVLA"
BASE COUNT          494 a         1136 c          932 g          515 t
ORIGIN      
        1 cgcgtggtag gtgtgcggcg tgtccgccag cgtgcgcgtc ggagggccgc tctgcgctcc
       61 gaatccgtgg aaacaaggct tcacgcggcg cacattctcc tccgagcggc ttgtgaccac
      121 cacgcgcgct ccctgccgcg ccagcgcccg cgccgccgcc agacccaggc cggtcgtccc
      181 gcccatgacc agcacgctca gaccccgcac atcgtaggtt gcattcatcc cgcaaaacat
      241 cccccgcgcc cccgcgcgca caactgccac tttgcattcg ccacgttttg ttttaacgcc
      301 tcccccggcc acagcggccg gaccgcatcc ccatcaccag gcacccacca tgatccgcgt
      361 ccgtttcgcc ccatccccca ctggctacct ccacgtcgga ggcgcgcgca ccgccctttt
      421 caactggctc ttcgcccgcc accacggcgg caaattcatc ctccgcatcg aggacaccga
      481 cgaagcgcgc aacaccgacg ccgcccgcca ggccattctc gacggtctcc gctggctcgg
      541 cctcgactgg gatgaaggcc ccgaagccgg cggcgaccac ggcccctatt tccaaagcca
      601 gcgcaagtcc gtctatgacg ccgcactcgc ccggctcgaa gccgccggcc gcacctacac
      661 cgaggaaaac ggcgccgtcc gtttcaaatt cagccgcacc gccatcaccg tgcgcgacct
      721 cgtctgcggc gatgtcacct tcgcccccac cgaagagccc gacatgaccc tccgtcgccc
      781 cgacggcagt tacatcttcc acttcgtcaa cgtcgtggac gacgccgaaa tgggcatcac
      841 ccacgtcatc cgcggcgagg accacctctc caacacctgg aagcacatgg acctcttcgc
      901 cgcccttggc aaagacgctc ccgcctacgc ccacatcccc ctcattctga accccggcgg
      961 ctccaaaatg agcaagcgcg acgaaggcgc ctccatccag agctatgtgg actccggctt
     1021 cctccccgac gccgtcttca actacctctg cctcctcggc tggacgcccc gcgccgaggg
     1081 cgaaaagctc gaccgcgcca ccctcgtcag cctcttcgac cccgccggcg tccacagcgc
     1141 caacgcccgc ttcgacatgc acaagtgcgc ctggttcaac gcccaatacc tgcgcgagct
     1201 cgaccccgcc gccctgctcg ccgccgccag gccctttctc gaaatggccg gcatcatcgt
     1261 caccgatgac tccctcgccg ccgccgccct ccaaagcgtg aaggaaaaag tcagccgcct
     1321 cgccgaattc cccgaatggg tccactactt cttccgcgag gactacccca cggaggaaga
     1381 cgccctcgcc aagctgcggg ccaagcccga aaaccccgcc ctgctccagg ccgcttccgc
     1441 cagcttccag gctctgaccg aatggaccga gcccgccgtc cacaccgcca tcgaggatgc
     1501 cgccaagggc gccggcgtaa aacccggcgc cctcatgccc ctgctgcgct ttgccctcag
     1561 cggccagact cgcggcccag gcgtcgccac catcgcccac ctcatcggca aagagcggac
     1621 acttgggcgc atccagagcg cgctggcgag gatggggtga aaacctccgc gaccgcgtgt
     1681 gtcgaatgac acctcacgcc agcacctcgc ggatgacttt tccgaaatcg cgctccaggc
     1741 gtttgcgtcc ggggatttcc attcggtggg ggttgaggcc gagcaggtgc agcacggtgg
     1801 cgtggatgtc ggtgacgtag tgcggatgct ccacggcgtg gaagcccagt tcgtcggtgg
     1861 cgccgtggat gaccccgcct ttgaccccgc cgccggccat ccagacgctg aagccgtagg
     1921 gatggtggtc gcgcccgtcg gtgccttgcg agcctggcgt ccggccaaac tcggtgccga
     1981 agacgacgag ggtgtcatcc agcaggccgc gctgcttgag gtctttcaaa agaccggaga
     2041 cgggcttgtc cacctgctcc gcgagggcgg agtggttttt tttcaagccg gagtgcgagt
     2101 cccacgcgcc ggctgcgccg tcgccatgca tgatctggat gaagcgcacg ccgcgctcgg
     2161 ccaggcggcg ggccgtgagg agctggcggg cgaagggctc ggtgacttta tggtcgagtc
     2221 cgtagaggcg ctttgtctcc tcgctttcgc tgtccaggtc cagcgtctcc ggcacggagg
     2281 tctgcatgtt gccggccagt gtgtagctcc tcatgcgcgc ggcgagcatg gcatcgccgg
     2341 ggtgctgcct ggcggtgatg gcgttcagcc gctggatgag ctggaactgc gcggcctgct
     2401 cgcgcgcgct ggcgccgcct tcggacacgg cgaaggcgag cggatttttg ggatccacgc
     2461 tgagcttcac ggcatcgtgc gcggggccga ggtaatggcc gtcgcgcgtg tcgaagtagc
     2521 gcgggcccat gccgatgaag gagggcaggt tgtcggtcat gctgccgagg ccccagttca
     2581 cccacgcgcc gagggtgggc gcgcgcggct ccagcatgtg gcggccgctg tggaactgca
     2641 cctgcgcccc gtggttgtca tccgtggtcc acatgccgcg gatgaaggcg atctcgtcgg
     2701 cgcaggagcc gatgtgcgga aaccagtcgc tgatctccac gccgcaccgg ccgtattttt
     2761 tgaagccggt ctgcagcggg taaatgacgt tgcgctgctg gccgttggcg tcgttcacca
     2821 ccaccacgcg cactttcttc agcttctcgg gatcctgcac gctggcgaag ggcgtctcgc
     2881 cgatgctctt gcccgcgtgg cggttcagag cgggcttcgg atcaaagctc tccatgtggc
     2941 tgacgccgcc gcgcatgaag agccagatga cgcgcttcgc cttcggcgcg tgcacgccgg
     3001 gtcggtgcgc gccatcagcg tgaagacgcc ggcgctgggc gcattcgtga tgtagcgctt
     3061 gctgccgtcg acgacat
//
LOCUS       DAOMYJ010000390         3058 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Prosthecobacter sp. isolate AMR_MDS_5629
            NODE_11762_length_3058_cov_19.470196, whole genome shotgun
            sequence.
ACCESSION   DAOMYJ010000390 DAOMYJ010000000
VERSION     DAOMYJ010000390.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867850
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prosthecobacter sp. (wastewater metagenome)
  ORGANISM  Prosthecobacter sp.
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Verrucomicrobiaceae; Prosthecobacter.
REFERENCE   1  (bases 1 to 3058)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3058)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.30
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:34:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,742
            CDSs (total)                      :: 4,684
            Genes (coding)                    :: 4,676
            CDSs (with protein)               :: 4,676
            Genes (RNA)                       :: 58
            tRNAs                             :: 55
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3058
                     /organism="Prosthecobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11762_length_3058_cov_19.470196"
                     /isolate="AMR_MDS_5629"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1965333"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1092
                     /locus_tag="PLP58_22150"
     CDS             <1..1092
                     /locus_tag="PLP58_22150"
                     /inference="COORDINATES: protein motif:HMM:NF024816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HRJ11299.1"
                     /translation="DTARATRALREGGALIVKDRHPLLWADYHQPLAEYLIEQAHWQE
                     ADDLLGEILDIREEHATTHPAELAGALLTWSKLLLDTAKFQGAHDVARRAAEIFGEIS
                     PPDAMSRAGAQSNQACALYEMGQIDQAEPIFARTLEFHETVLGQHDPLVAVSLNNLAK
                     IHQEKGRLRQAETMFRRALAIQEAVYGAHHGKLVNRLDNLAALIETSGRVEEAEALHR
                     RALGILESAYGTRHPRVAQSLSNLGLLLTRAQRFAEAEVFLRRAVEIDRECFEPDSIR
                     NVLALNNLALLLHETGRLQESLRLYERAVKLVMRYHLVYQHPSRHEDHVMANCSGVLW
                     DMGWDDAAVMRHLGGLRQEVAQDLRHRHE"
     gene            1771..1914
                     /locus_tag="PLP58_22155"
     CDS             1771..1914
                     /locus_tag="PLP58_22155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ11300.1"
                     /translation="MSRNAVPRNNKRLLARAEDCADGAHELEDEIPLVLIRESNLRAQ
                     IRG"
     gene            complement(2178..>3058)
                     /locus_tag="PLP58_22160"
     CDS             complement(2178..>3058)
                     /locus_tag="PLP58_22160"
                     /inference="COORDINATES: protein motif:HMM:NF024762.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transglutaminase-like domain-containing protein"
                     /protein_id="HRJ11301.1"
                     /translation="RRRELERQAAALRDLEKDLHRRAVTRDLLAELAKPEDKADLMRA
                     TLLLARHDNPEIEIRHYMQAFTRMVDELRHDPAIAKGTLPAIARLNEYLFEQGGFHGS
                     RHDYESRSNSYMNEVLDDREGLPITLSVLYLELASRLGIPHVFGAPLPGKFMVAYRDG
                     PEGELKLLDVFERGKTLTVEEAALQLTRTGELDESFLQPATKKSIILRMLRNLLGGAL
                     DDESSVKESLPYLDLLLSIDPQAAVERLTRARMNQRLGHKDAAARDVEWLMENFPQDG
                     PDQLRLQLEQWLDALR"
BASE COUNT          538 a          966 c         1025 g          529 t
ORIGIN      
        1 gacaccgccc gcgccacccg cgccctgcgc gagggcggcg ccctcatcgt caaagaccgc
       61 caccccctcc tctgggccga ctaccaccag cccctcgccg aatacctcat cgagcaggct
      121 cactggcagg aagccgacga cctcctcggc gaaatcctcg acatccgcga ggagcacgcc
      181 accacccacc ccgccgagct ggccggtgcg ctcctgacct ggagcaaact cctgctcgac
      241 accgccaaat tccagggcgc gcacgacgtc gcccgccgcg cggcggagat ttttggtgaa
      301 atctcgcccc cggacgccat gagccgggcc ggcgcgcagt ccaaccaggc gtgcgccctt
      361 tatgaaatgg gccagattga tcaagccgag ccgattttcg cgcggacttt ggaattccat
      421 gaaaccgtcc tcgggcagca tgatccgcta gtcgccgtga gcctgaacaa cctcgccaaa
      481 atccaccagg aaaaaggccg gctgcggcaa gccgagacca tgttccgccg cgccctcgcc
      541 atccaggagg ccgtctatgg cgcgcaccac ggcaaactcg tcaacaggct cgacaatctc
      601 gccgctctca tcgagaccag cggccgggtg gaggaggctg aggcacttca ccgcagggcg
      661 ctgggtattt tggagtcggc gtatgggacg cgtcatccaa gagttgccca aagcctcagc
      721 aatttgggcc tgctgctgac ccgcgcccag aggtttgccg aagcggaagt attcctgcgc
      781 cgcgcggtgg aaattgaccg ggaatgcttt gaaccagaca gcatcagaaa tgtcctcgcg
      841 ctgaacaatc tggctctgct tcttcacgaa accggcaggc tgcaggagtc cctccggctc
      901 tacgagcgcg ccgtgaagct ggtcatgcgt tatcatctcg tctaccagca cccctcccgg
      961 catgaggatc atgtcatggc aaactgctcc ggcgttttgt gggacatggg ctgggacgat
     1021 gccgccgtca tgcgccatct gggtggcttg cggcaggaag tggcgcagga cctgcgccac
     1081 aggcacgaat gaccgtaaaa ccctgactcg tgcggcagcc ccgcctccgc agggcgggcg
     1141 gatgtgtttt ttcacccccg ccgcattttt ttctcgactt ccggcgcgcg ttccggtgaa
     1201 atgcttttta ttccgcgcgc ccaaccccgc gcggtccgcc gccgatgatc gccaacgcgc
     1261 tttcctggcc acgggcaggc tgccgcgcca cccgcagggc ggagccgttg caggatgccg
     1321 cgctccaaat gcacgccgca ttcgccgccc gcgccggtga cagggcacaa atgcacggtg
     1381 catttgccac ccgcagcggc aacacatgcc aaatgcacgg tgcatttgcc acctgcagcg
     1441 acaacacatg ccaaatgcac ggtgcatttg ccacctgctg cgacgacaca tgccaaatgc
     1501 acggtgcatt tgccacccgc agcgacgaca cacgccaaat gcacgccgca ttgagagcag
     1561 gcgggcgggg ccttgatgct ttttttcacc gggtggtgcc gccacccgcc cacctgccac
     1621 cgccgcatga aaatcattac cttggacaca tgggtttcct aggatggttt caatgcccga
     1681 tgaggtgatc gagcctgcct gcttgagccg ggtgatccgg gttaacaggg gccggttcca
     1741 agtccggcag acatcaaacg caaaaccaaa atgagccgca acgccgtccc aagaaacaac
     1801 aagcgcctgc tggcgcgggc cgaggactgc gccgacggcg cgcatgagct ggaggatgaa
     1861 atacccctgg tgctgatccg cgagtcgaat ctgcgcgcgc aaatacgggg ctgaaatccg
     1921 tgtgtgtatc tttcacacac ggagccagct ttggagcagc ggctcatcgg agtgacgcgc
     1981 ttccggcact cttcccggca gatggagccg ggccatgcgc acagaggggc cggcgctttg
     2041 tccgaacctc ccgcgcggaa gcgtcccgga gtgcgcggca aaaccctgaa ggggtggcga
     2101 cgcggctccg cgcgggtgtg taaactgtct ggctgaaggc cgcccccttc ctgacggggt
     2161 gggggttgcg gcggcgctca gcgcagggcg tcgagccatt gctcgagctg gaggcggagc
     2221 tggtcggggc cgtcttgggg gaagttctcc atgagccatt cgacatcccg cgcggcggcg
     2281 tccttgtggc cgaggcgctg gttcatgcgg gcgcgggtga ggcgctcgac ggcggcctgg
     2341 gggtctatgc tgaggaggag gtcgaggtaa gggagggatt ccttgacgga ggattcgtcg
     2401 tcgagggcgc cgccgagcag gttgcgcaac atgcgcagga tgatggattt tttcgtggcg
     2461 ggctggagga aggactcgtc cagctcgccg gtgcgggtga gctggagggc ggcctcctcg
     2521 acggtgaggg ttttgccgcg ctcgaagaca tcgagcagct ttaactcgcc ctcgggtccg
     2581 tcgcggtagg cgaccatgaa tttgccgggc agcggggcgc cgaagacgtg ggggatgccg
     2641 aggcgggagg ccagctccag atagagcacg gagagggtga tgggcaggcc ctcgcggtcg
     2701 tcgaggacct cgttcatgta gctgttggag cggctttcgt agtcgtgccg gctgccgtgg
     2761 aagccgccct gctcgaagag gtattcattg aggcgggcga tggcggggag ggtgcctttg
     2821 gcaatggcgg ggtcgtggcg cagctcgtcc accatgcggg tgaaggcctg catgtagtgg
     2881 cggatctcga tctcggggtt gtcatgccgg gccaggagca gggtggcgcg catgaggtcg
     2941 gctttgtcct cgggcttggc cagctcggcg aggaggtcgc gggtgacggc gcggcggtgg
     3001 aggtcttttt ccaggtcgcg cagggcggcg gcctggcgct ccagctcgcg gcggcgct
//
LOCUS       DAOMYJ010000391         3056 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Prosthecobacter sp. isolate AMR_MDS_5629
            NODE_11777_length_3056_cov_16.274575, whole genome shotgun
            sequence.
ACCESSION   DAOMYJ010000391 DAOMYJ010000000
VERSION     DAOMYJ010000391.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867850
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prosthecobacter sp. (wastewater metagenome)
  ORGANISM  Prosthecobacter sp.
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Verrucomicrobiaceae; Prosthecobacter.
REFERENCE   1  (bases 1 to 3056)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3056)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.30
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:34:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,742
            CDSs (total)                      :: 4,684
            Genes (coding)                    :: 4,676
            CDSs (with protein)               :: 4,676
            Genes (RNA)                       :: 58
            tRNAs                             :: 55
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3056
                     /organism="Prosthecobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11777_length_3056_cov_16.274575"
                     /isolate="AMR_MDS_5629"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1965333"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..226)
                     /locus_tag="PLP58_22165"
     CDS             complement(<1..226)
                     /locus_tag="PLP58_22165"
                     /inference="COORDINATES: protein motif:HMM:NF012610.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HRJ11302.1"
                     /translation="MSDSESLPMRPSLVAETARVLREGISSGRWPERLPGERELSAAL
                     QVGRNTLRAALAELEREGCQTTLGDAVGDVL"
     gene            359..1108
                     /locus_tag="PLP58_22170"
     CDS             359..1108
                     /locus_tag="PLP58_22170"
                     /inference="COORDINATES: protein motif:HMM:NF017532.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phytanoyl-CoA dioxygenase family protein"
                     /protein_id="HRJ11303.1"
                     /translation="MNIPHHPGSAALVAGYETDGVVRVPGFLQSDEVAAARAELDRYI
                     REDLPSRPADARTFEKDDKTVRNLWRLEQHNEFFRALGLRPDILALIAPLVNGDPVLC
                     AVETFNKPALIGSGVPPHQDNAYFCQTPPDMLTVWIAIDAVTEANGPVFFIKGSHLEG
                     MRPTRPSGVRGNSIGLAESPAVPLPEQYCGLLQPGDATIHHCQTIHHSAPNTTPHSRL
                     GLLLVYRGSHTRTDPALQAAYSAAAAATPPA"
     gene            1133..2665
                     /locus_tag="PLP58_22175"
     CDS             1133..2665
                     /locus_tag="PLP58_22175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ11304.1"
                     /translation="MTLKSALLLPLLALAAHGAETTFFAFDDHNIAWQHNLKLTLAAA
                     EKHPANPVLRAGPPGSPDHGHAILYGTVLKQDGIFKMWYLGMHETENKAGQAPGWWRP
                     MCYAESRDGVTWTKPALGLVEFNGGTANNICLIEGGVHSMTRVNDFLSILHEPDDPDP
                     DPDRRYKCAFIAHMPFEEVKGGRSKIGPDERRWGAFITATSADGLRWKVVGDRPVNAG
                     GERFEVSGLYRFGDFYHATGQLISPWSWRADGSQVGRDMLAYRSPDFTTWSKAKARSF
                     IRPGQAANPPVKGQQMHMGAGLWNRGNVIVGLHGMWQDAAQPPPKGASWNLGVRIDLG
                     LLVSNDGVHFREPVPGHAVIPRGRDGEWDDIAILQGHAFVNEGDRTLIWYSHWDTGGR
                     LKDMHIGLATLRRDGFGHLSRQVSEEEAHFITSTFEASAIALNVDGITAGAPLTLQLL
                     DHLDRPLEGMTASLDQNGVRVPVTWPRPLPAGKKTALRVHYPANSAARVYAVYVTRGV
                     GS"
     gene            complement(2716..>3056)
                     /locus_tag="PLP58_22180"
     CDS             complement(2716..>3056)
                     /locus_tag="PLP58_22180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ11305.1"
                     /translation="LGGRLTHRDGALVDWGDTADWASWLVEIPAAGDYEILIHQAHAG
                     PETGTYEVLLAGQVLPAAVVATGAEFKGFILGTVAVPAPGIYKVFLRPRKLPASGPLF
                     QVRGLALKEG"
BASE COUNT          528 a         1250 c          856 g          422 t
ORIGIN      
        1 gcagtacatc gccgaccgca tccccgagag tggtctggca cccctcccgc tccagctcag
       61 ccagcgccgc gcgcagcgtg ttgcgcccca cttgcagcgc cgcgctcagc tcgcgctcgc
      121 ccggcaggcg ctccggccag cgtcccgaac tgattccctc cctcagcacg cgcgccgtct
      181 ccgccaccag cgaagggcgc atcggcaggg attcggagtc gctcatcccg ccatgctggc
      241 aggggcgggc gtgaaaggca aactctgatc ccgccccctg gttccatgcg gaaaccagtc
      301 cggcaggctt gtccgctgcg ggcggagaca gttccctgcg cgcgtccatt cgcagcccat
      361 gaacattccc caccacccag gcagcgccgc gctcgtcgca ggctatgaga cagacggcgt
      421 cgtccgcgtc cccggcttcc tccagtcgga tgaagtggcg gccgcgcgcg ccgagcttga
      481 ccgctacatc cgcgaggacc tgccctcccg gcctgcggac gcgcgcacct tcgaaaagga
      541 cgacaaaacc gtgcgcaacc tctggcgcct ggagcagcac aacgaattct tccgcgccct
      601 cggcctgcgt ccggacatcc tcgcgctcat cgccccgctg gtgaacggcg accccgtcct
      661 ctgcgccgtg gagaccttca acaaacccgc cctcatcggc tccggcgtcc cgccccacca
      721 ggacaacgcc tacttctgcc agacgccgcc ggacatgctc accgtctgga tcgccattga
      781 cgccgtcacc gaggcgaacg gccccgtctt tttcatcaaa ggctcccacc tcgaaggcat
      841 gaggcccacc cgccccagcg gcgtccgcgg caacagcatc ggcctcgccg aaagccccgc
      901 cgtgccgctg ccggagcaat actgcggcct gctccagccc ggagacgcca ccatccacca
      961 ctgccagacc attcaccact ccgcgcccaa caccaccccg cattcccgcc tcgggctcct
     1021 gctggtctat cgcggctccc acacccgcac cgatcccgcc ctgcaggccg cctacagcgc
     1081 cgccgccgcc gccacaccgc ccgcctgaat ccgcccccgc acccccgcag ccatgactct
     1141 gaaatccgcg ctcttgctcc cgctgctcgc cctggccgcc cacggggccg agaccacatt
     1201 cttcgccttc gacgaccaca acatcgcctg gcagcacaat ctcaagctca ccctggccgc
     1261 cgccgaaaag cacccggcca atcccgtcct gcgcgccgga ccgcccggca gccccgacca
     1321 cggccacgcc atcctctacg gcaccgtcct caagcaggac ggcatcttca aaatgtggta
     1381 tctcggcatg cacgagaccg aaaacaaagc cggccaggcc cccggatggt ggcggcccat
     1441 gtgctacgcg gagagcaggg acggcgtcac ctggaccaag cccgccctcg gcctcgtcga
     1501 attcaacggc ggcaccgcca acaacatctg cctcatcgag ggcggcgtgc attcaatgac
     1561 ccgcgtcaac gacttcctct ccatcctcca cgagccggac gaccccgacc ccgaccccga
     1621 tcgccgctac aaatgcgcct tcatcgccca catgcccttc gaggaagtca aaggcggccg
     1681 cagcaaaatc ggccccgacg agcgccgctg gggcgccttc atcaccgcca ccagcgccga
     1741 cggcctgcgc tggaaggtcg tcggcgaccg ccccgtcaac gccggcggcg agcgcttcga
     1801 ggtcagcggc ctctaccgct ttggcgactt ctatcacgcc accggccagc tcatcagccc
     1861 ctggtcctgg cgcgccgacg gcagccaggt cgggcgcgac atgctcgcct accgctcccc
     1921 ggacttcacc acctggtcca aggccaaagc ccgctccttc atccgccccg gccaggccgc
     1981 caacccgccc gtcaaaggcc agcagatgca catgggcgcc ggcctttgga atcgcggcaa
     2041 cgtcatcgtc ggcctccacg gcatgtggca ggacgccgcc cagcccccgc ccaaaggcgc
     2101 gagttggaac ctcggcgtcc gcattgacct cggcctgctc gtcagcaacg acggcgtcca
     2161 tttccgcgaa cccgtccccg gccacgccgt catcccgcgc ggcagggacg gcgagtggga
     2221 cgacatcgcc atcctccagg gccacgcctt cgtcaacgag ggggaccgca ccctgatctg
     2281 gtactcccac tgggacaccg gcggcaggct caaggacatg cacatcggcc tggccaccct
     2341 ccggcgcgac ggcttcggcc acctctcgcg ccaagtcagc gaggaagaag cccacttcat
     2401 caccagcacc ttcgaagcct ccgccatcgc cctcaacgtg gacggcatca ccgccggcgc
     2461 cccgctcacc ctgcagctcc tcgaccacct cgaccgcccg ctcgaaggca tgaccgcgag
     2521 cctcgaccag aacggcgtcc gcgtccccgt cacctggccg cgacccctgc cggcaggaaa
     2581 gaaaaccgcc ctccgcgtcc actaccccgc caacagcgcc gcgcgggtct atgccgtgta
     2641 tgtcacacgc ggcgtcggat cgtgatggga aagctcgaaa attgaaatcg cggagtagtt
     2701 ttcgaatttc aaatttcacc cctctttcag cgccaggccg cgcacctgaa acagcggccc
     2761 cgaggccggc agtttgcgcg ggcgcaggaa gaccttgtag atgccgggag cggggacggc
     2821 cacggtgccg aggatgaagc ccttgaactc cgcgccagtg gcgaccaccg ccgccggcag
     2881 cacctgcccg gccagcagca cctcataagt gccggtctcc ggccccgcgt gcgcctggtg
     2941 gatgagaatc tcgtagtcac cggcggcggg gatttcgacg agccatgagg cccagtccgc
     3001 cgtgtcgccc cagtccacca gcgcgccgtc gcggtgggtg agcctgccgc ccaggc
//
LOCUS       DAOMYJ010000392         3031 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Prosthecobacter sp. isolate AMR_MDS_5629
            NODE_11912_length_3031_cov_19.655578, whole genome shotgun
            sequence.
ACCESSION   DAOMYJ010000392 DAOMYJ010000000
VERSION     DAOMYJ010000392.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867850
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prosthecobacter sp. (wastewater metagenome)
  ORGANISM  Prosthecobacter sp.
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Verrucomicrobiaceae; Prosthecobacter.
REFERENCE   1  (bases 1 to 3031)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3031)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.30
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:34:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,742
            CDSs (total)                      :: 4,684
            Genes (coding)                    :: 4,676
            CDSs (with protein)               :: 4,676
            Genes (RNA)                       :: 58
            tRNAs                             :: 55
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3031
                     /organism="Prosthecobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11912_length_3031_cov_19.655578"
                     /isolate="AMR_MDS_5629"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1965333"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..479)
                     /locus_tag="PLP58_22185"
     CDS             complement(<1..479)
                     /locus_tag="PLP58_22185"
                     /inference="COORDINATES: protein motif:HMM:NF013094.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008641 - ubiquitin-like modifier
                     activating enzyme activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ThiF family adenylyltransferase"
                     /protein_id="HRJ11306.1"
                     /translation="MNFERALLCGLGNTGSALLPLLAALPGLREVVLVDRDRIERANL
                     ACQAGLTAADVGRSKARVHARRLRGLRPGLKVVAYDQDLARIPLGEWHGALVVGCLDN
                     NEARVVLNAGAFRARAALLLDLGVRADNRLARVSVLHPGRAGSACLECSWSEADYTPA
                     "
     gene            complement(476..796)
                     /locus_tag="PLP58_22190"
     CDS             complement(476..796)
                     /locus_tag="PLP58_22190"
                     /inference="COORDINATES: protein motif:HMM:NF025802.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RING finger protein"
                     /protein_id="HRJ11307.1"
                     /translation="MSVNSEDAAVTGIRVLRHKDMICAEGLRLFFSGEKIAAVSALEA
                     ADVHCARCQSEMQTGTPVLLCPECGAPHHAEGCWDAAPACALCPRPTNARGLLWSPVE
                     GGWS"
     gene            1171..1623
                     /locus_tag="PLP58_22195"
     CDS             1171..1623
                     /locus_tag="PLP58_22195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ11308.1"
                     /translation="MAAPSPHSADIKRAFELFFDARRLLEEGYMTYFKAEEKLDLAES
                     LLRNIGGDAVAGQPQLEIKVSVMEASINGSHIPLSPAQLKLMNLLNESPGMTGREELQ
                     RLMNLNSHGLSVLMNKLKAKLRDAGFPDVIIQENNAYRLRKIQPVTDG"
     gene            1801..2625
                     /locus_tag="PLP58_22200"
     CDS             1801..2625
                     /locus_tag="PLP58_22200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ11309.1"
                     /translation="MPAQTSTLPDLSTVAADLSVPEITEGPPAAGKRVYAMASGWENT
                     RVRHLLYLPADWQPAGAPLPVLVEYAGNGGYKNAFGDACDGGVEGCRLGYGISGGRGF
                     LWLCLPYVEPHEDGKRNAVKWWGDVQESKRYCLAAVRETCARYGGDPARVVLCGFSRG
                     SIGCNFLGLHDDEIAALWRAFICHSHYDGVSERWPYAGADRASSLQRLARLRGRPQFI
                     SHEGGTGAIETWLISTGIEGAWTFTPIPFRNHSDAWVLRDLPERARLREWLARALE"
     gene            complement(2669..>3031)
                     /locus_tag="PLP58_22205"
     CDS             complement(2669..>3031)
                     /locus_tag="PLP58_22205"
                     /inference="COORDINATES: protein motif:HMM:TIGR02464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADAR family protein"
                     /protein_id="HRJ11310.1"
                     /translation="VKWRSVEHCYQAAKYEDASIVKIIHSASSPLAARKAGQNRSLTP
                     RQDWDENKIRVMTEAVRAKFTQNRRLREQLLATGDEELVHLSRNDLFWGRTAEGGGEN
                     RLGLILMEVRSELRRQDG"
BASE COUNT          577 a          997 c          928 g          529 t
ORIGIN      
        1 gcgggcgtgt agtcggcctc ggaccacgag cactctaggc aggcggaccc ggcgcggccg
       61 ggatgcagca cagagacgcg agccaggcgg ttgtcggcgc gcacccccag gtccagcagc
      121 aaggcggcgc gcgcgcgaaa ggcgccggca ttgagcacga ccctggcctc gttgttgtcc
      181 agacagccca ccaccagcgc gccatgccac tcgccgagcg ggatgcgcgc caggtcctga
      241 tcgtaggcca cgactttcaa gcccggacgc agcccgcgca gccgccgggc atgcacgcgg
      301 gccttggacc tgccgacatc cgccgcggtg agacccgcct ggcaggccag attggcgcgc
      361 tcaatccggt cccgatccac aagcaccacc tcgcgcaagc ccggcagcgc ggccagcaaa
      421 ggcaggaggg ccgagccggt gttgcccagc ccgcagagca gcgcgcgctc gaaattcatg
      481 accagcctcc ttccaccggc gaccagagca ggccgcgcgc attcgtcggc cgcgggcaca
      541 gcgcgcaggc gggcgcggca tcccagcagc cctccgcatg gtgcggcgcg ccgcactccg
      601 ggcacagcag aacgggagtt cccgtctgca tctccgactg gcagcgcgcg cagtggacgt
      661 ccgccgcctc cagcgccgag acggcggcga ttttttcgcc gctgaaaaac aaccgcagcc
      721 cctccgcgca gatcatgtcc ttgtggcgca gcacccggat gccggtgaca gcggcatcct
      781 cgctattgac actcactctt gaatcaggag cggccagcag cacccattcg gctgagccgg
      841 aaccacacgc ggagtggatg gacaaacctt gcgcgcccag acacacggcg cgccccaccg
      901 gcaaaagcac acactcccac cggggggagg cagaggacag caagggcggc agagacggcc
      961 cggcatgaga gaagaacagt tgcggcatgg ctcgaatttt ttcaaatttt agcctgccat
     1021 ccggcatcac cggtgatcag tgcgcagaat caccaaaaaa aatctgacgg tgccagatct
     1081 tgccgtgggc ggtcaccgtg tgccgtgaaa tagaatcgtt aacatatcga gccatggcgg
     1141 gataccaggt gaatcaaccc caccttcaac atggccgcac cgtctcccca ctcagccgac
     1201 atcaaacgcg catttgaact gtttttcgat gccaggcgtc tgctcgagga gggttacatg
     1261 acctacttca aggccgagga aaaactcgac ctcgccgagt cgcttttgcg caacatcggc
     1321 ggtgatgccg tggctgggca gcctcaactc gagatcaagg tttcagtgat ggaagcatcc
     1381 atcaacggct cgcacatccc gctttcgccg gctcagctca aactcatgaa tttgttgaat
     1441 gagtctcccg gcatgacggg cagggaggag ttgcaacggc tcatgaacct caactcgcac
     1501 ggcctttccg tgctcatgaa caagctcaaa gccaaactca gggacgcagg cttccctgat
     1561 gtgatcattc aggaaaacaa cgcctaccgg cttcgtaaaa tccagcctgt gactgatggc
     1621 tgaccggtct ttcgaacccg ctttggcatc cagacctcag tgcaggcttc ccagcttgca
     1681 cacaagacgg gtgtcattgc gcaagattgt cattgatggt gtctctcgaa tatgcttcgc
     1741 ccgcccatga atttcattca gtccttgtcc gcatgtcttt tcttgctgct tcgaacgctc
     1801 atgcccgccc agacatccac gctgcctgat ctctccactg tagccgcaga cttgagcgtg
     1861 ccggaaatca cggagggacc gcctgccgcg ggaaaacgtg tgtatgccat ggcctcgggc
     1921 tgggagaaca cgcgggtgcg gcacttgctt tacctgcccg ccgactggca gccggcaggc
     1981 gcgccactgc cagtgctggt ggagtacgcg ggcaatggcg gttacaagaa cgcattcggt
     2041 gatgcctgcg acggcggtgt ggaggggtgc cggctcggct atggcatcag cggcggcagg
     2101 ggatttctct ggctgtgcct gccctatgtg gagccgcacg aggacggcaa acgcaacgcc
     2161 gtgaaatggt ggggcgatgt gcaggagagc aagcgctact gcctggccgc cgtgcgcgag
     2221 acctgcgcgc gttacggagg cgaccccgcg cgcgtggtgc tgtgcggctt ctcgcgcggc
     2281 agcatcggct gcaatttcct cgggttgcac gatgacgaga ttgcggcgct ttggcgcgcc
     2341 ttcatctgcc acagtcatta tgacggcgtg agcgaacgct ggccgtatgc gggcgcggac
     2401 cgagcctcgt cgctgcaaag actggcccgg cttcgcggca ggccgcagtt catcagccac
     2461 gagggcggca ccggcgccat cgaaacatgg ctgatctcca cggggatcga aggcgcgtgg
     2521 actttcacgc cgatcccctt ccgcaatcac agcgacgcat gggtgctgcg cgacctgccg
     2581 gagcgcgccc gcctgcgcga atggctggcg cgcgcgctgg aatgacggac tcgatgggct
     2641 ggcacgggag gcattctccg agctggcatc atccgtcctg cctgcgcaat tcgctcctga
     2701 cctccatcag gatcagtccc agccggttct cccccccgcc ctccgctgtg cgaccccaga
     2761 acaaatcgtt gcgcgaaagg tggaccaact cctcgtcacc cgtcgccagc agttgctccc
     2821 gcaaacgccg gttctgtgtg aacttggctc gcacagcctc cgtcattacc ctgatcttgt
     2881 tttcatccca atcctgcctc ggagtgaggg accggttctg accggctttg cgcgccgcca
     2941 ggggtgatga ggctgaatgg atgattttga cgatggaggc gtcctcgtat ttggcggcct
     3001 gataacagtg ctccacactg cgccacttca c
//
LOCUS       DAOMYJ010000393         3029 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Prosthecobacter sp. isolate AMR_MDS_5629
            NODE_11928_length_3029_cov_19.565568, whole genome shotgun
            sequence.
ACCESSION   DAOMYJ010000393 DAOMYJ010000000
VERSION     DAOMYJ010000393.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867850
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prosthecobacter sp. (wastewater metagenome)
  ORGANISM  Prosthecobacter sp.
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Verrucomicrobiaceae; Prosthecobacter.
REFERENCE   1  (bases 1 to 3029)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3029)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.30
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:34:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,742
            CDSs (total)                      :: 4,684
            Genes (coding)                    :: 4,676
            CDSs (with protein)               :: 4,676
            Genes (RNA)                       :: 58
            tRNAs                             :: 55
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3029
                     /organism="Prosthecobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11928_length_3029_cov_19.565568"
                     /isolate="AMR_MDS_5629"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1965333"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            346..858
                     /locus_tag="PLP58_22210"
     CDS             346..858
                     /locus_tag="PLP58_22210"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009963756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HRJ11311.1"
                     /translation="MPLLAAFYSSLFGLAKAGPGESVDLPAPEVAGINQDGREVRFAE
                     VYARGPTVVFFYPKAGTPGCTAQACSLRDAFAELHKQGVQVLGVSTDGQAAQKKFRGD
                     HKLPYDLIADPEGKILAAFGVGKVLGGLLPYSSRQCFLIKGGRVIWRDTSASTSQQAD
                     DILRVLARGQ"
     gene            985..1905
                     /locus_tag="PLP58_22215"
     CDS             985..1905
                     /locus_tag="PLP58_22215"
                     /inference="COORDINATES: protein motif:HMM:NF013429.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HRJ11312.1"
                     /translation="MNRRHFLASSLGLSAVSTLSAIDPINRSGASRMQLGVAAYSFRD
                     FFQWSRGKEQKPAEGRKPWSILDFIDWCADHNVPGAEVTSYFFPPDVDAAFLLEVRRH
                     AYLRGVQLAGTAVGNNFALPKGEKLDAEIAAVKQWIDHAAVMGAPHIRVFAGPVPKDL
                     TIEEAVANCSAAYQECLDYAGEKGVFLGLENHGGIVAEPENLVKMVKDARSPWAGINF
                     DSGNFHTEDPYGDLAKIAPYAINVQLKMVMRPKGVERNQPGEPTDIPRVLKILREANY
                     QGWFTIEFEEKKDPFEELPKIIEKLRPLLA"
     gene            complement(2011..2934)
                     /locus_tag="PLP58_22220"
     CDS             complement(2011..2934)
                     /locus_tag="PLP58_22220"
                     /inference="COORDINATES: protein motif:HMM:NF016777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HRJ11313.1"
                     /translation="MTTSRRRFLSTSLAAAAAGNLTQCASSGAAGAVDAHVHVWTPDT
                     ARYPLAPGFARADMRPASFTPEQLFAHARPAGVSRVVLIQMSYYRFDNRYMLDSIAAH
                     PGVFAGVAIIDEHAPDVRGRMKALAARGVTGFRVHTGEKDIESWLGSPGMAEMWRVAA
                     DENLSICLLINPEALPAVDKMCARFPRTPVVVDHFARIGMSGAVDRAQLDRLLHLARH
                     PLAHVKTSAFYALGKKRPPYTDLGPMIRECRDAYGAQRLMWASDCPFQVGPGHNYPDS
                     IALIRGRLDFLSASDRAWMLRKTAERVFFRA"
BASE COUNT          563 a          986 c          955 g          525 t
ORIGIN      
        1 cgtccgccgg aaggcggcga acgtggcggc ttccgccgcc cccctgaagg tgagggcaca
       61 ggccgcccgc gtcctcccct ggaggatgag gcgccgaaaa aagacccggc ctgatcaaca
      121 gccagccgtt tgactcaaag gcggaattcc gaaagggatt ccgccttttc cgtgccgggg
      181 cagaacgcca gtgaatatca ggaaggcaac tttcccgcaa ggtggatgct ccaggtcctg
      241 agtttgtttg gagagtttgt cgcctgaccg atccaggaga atggcgggca ttccctgccg
      301 tccgtctgga cttgcccgcc gtatgctggc taaatgatcc gcatcatgcc cctgcttgcc
      361 gctttttatt ccagtctgtt cggcctcgcc aaagccggtc ccggcgagtc tgtggacctg
      421 cccgcgccag aggtggccgg catcaaccag gatgggcggg aggtgcggtt cgcggaggtc
      481 tatgccaggg ggccgaccgt ggtgtttttt tatccaaaag cgggcacgcc cggctgcacc
      541 gcgcaggcct gctcactgcg cgatgctttt gccgaattgc ataaacaggg cgtgcaggtg
      601 ctcggtgtct ccacggacgg gcaggcggcg cagaaaaaat tccgcggcga tcacaagctg
      661 ccctatgacc tgatcgccga tcccgaaggc aaaatcctgg cggcgttcgg cgtgggcaag
      721 gtgctcggcg ggctgctccc ctattcctca aggcagtgct tcctgatcaa gggcgggcgc
      781 gtcatctgga gggacaccag cgcctccact tcacaacagg cggatgacat cctgcgagtg
      841 ctggcgcgcg gccagtgatc tccggtgcgt ggcatggtga aaggatgccg ctgccgtcgc
      901 cagcgcggcc cggctgggta gaaaatctgt tcacattcgc ggaaagctgc tattcctgcg
      961 cttccaatcc atacttcccc acccatgaac cgccgtcatt tcctcgccag cagcctcggc
     1021 ctctccgccg tctccaccct ttccgccatt gaccccatca accgttccgg cgccagtcgc
     1081 atgcagctcg gcgtggccgc ctatagcttc cgcgattttt ttcaatggag ccgcggcaag
     1141 gagcaaaaac cagccgaagg ccgcaaaccc tggagcatcc tcgacttcat tgactggtgc
     1201 gccgaccata acgttcccgg cgcggaagtg acgagctatt tcttcccacc ggatgtggac
     1261 gccgccttcc tgctcgaagt gcgccgccat gcctacctgc gcggtgtgca gctcgccggc
     1321 accgccgtgg ggaacaactt cgcactgccc aagggggaga agctggacgc ggaaatcgcc
     1381 gccgtgaagc agtggataga ccacgccgcc gtgatgggcg cgccgcatat tcgcgtcttc
     1441 gccggccccg ttccgaagga tctcaccatc gaggaagcgg tggcgaactg ctcggcggct
     1501 tatcaggaat gcctcgacta cgcgggcgaa aaaggcgtct tcctcggcct ggaaaaccac
     1561 ggcggcatcg tcgccgagcc ggagaatctg gtaaagatgg tgaaggatgc gcgcagcccc
     1621 tgggccggca tcaacttcga cagcggcaac ttccacactg aggacccgta tggcgacctc
     1681 gccaaaatcg ccccctacgc catcaacgtg cagctcaaga tggtcatgcg ccccaaaggc
     1741 gtggaacgca accagcccgg cgagcccacc gacatcccgc gcgtgctcaa aatcctgcgc
     1801 gaggcaaact accagggctg gttcaccatc gagttcgagg agaagaaaga ccccttcgag
     1861 gagctgccaa agatcatcga aaaactgcgc ccgctgctgg cctgagcgcg ccgggcatga
     1921 tgcggacact gttgtccgca tcagttctct tcagaaaagt cagcccgatc tggacagact
     1981 ttggtgtctt tgccgcgccc gcacggcgtg tcacgcgcga aaaaaaaccc gttccgccgt
     2041 tttgcgcagc atccaggctc ggtcgctggc gctgaggaag tccagccgcc cgcggatgag
     2101 ggcgatgctg tccgggtagt tgtgaccggg gcccacctga aaggggcagt cactcgccca
     2161 catgagccgc tgcgcgccat aggcgtcacg gcactcgcgg atcatcgggc cgaggtcggt
     2221 gtaaggcggg cgttttttcc ccagcgcgta aaaggcggag gtcttcacat gcgccagcgg
     2281 atgccgcgcc aggtgcagca ggcggtcgag ctgggcgcgg tccaccgcgc cgctcatgcc
     2341 gatgcgggcg aagtggtcca ccaccaccgg cgtgcgcgga aagcgcgcgc acattttgtc
     2401 cacggcgggc agcgcctccg ggttgatgag caggcagatg ctcaggttct catccgccgc
     2461 cacccgccac atctcggcca tgccgggcga gccgagccag gactcgatgt ctttttcacc
     2521 ggtgtgaacg cggaaaccgg tgacaccccg cgccgccagc gccttcatgc gcccgcgcac
     2581 gtccggcgcg tgctcgtcaa tgatggccac gcccgcgaat actccgggat gcgccgcgat
     2641 actgtcgagc atgtaacgat tgtcgaagcg gtagtagctc atctggatca gcacgacgcg
     2701 cgacaccccg gcgggccgcg catgggcgaa gagctgctcc ggcgtgaagc tggcgggccg
     2761 catgtccgcc ctggcaaagc ccggcgccag cggataccgc gcggtgtccg gcgtccacac
     2821 atgcacatgc gcgtcaaccg cccccgctgc gccggagctg gcgcattggg tgagattccc
     2881 cgctgctgcg gcggcgagac tggtggagag aaatcggcgg cggctggtgg tcatgacagg
     2941 caagatttac gcgaaggtgg aaaggaatct tcgatggaac tccagggcgc gctccacctg
     3001 atccagctcg atgaattcat cggcggtgt
//
LOCUS       DAOMYJ010000394         2993 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Prosthecobacter sp. isolate AMR_MDS_5629
            NODE_12165_length_2993_cov_20.092920, whole genome shotgun
            sequence.
ACCESSION   DAOMYJ010000394 DAOMYJ010000000
VERSION     DAOMYJ010000394.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867850
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prosthecobacter sp. (wastewater metagenome)
  ORGANISM  Prosthecobacter sp.
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Verrucomicrobiaceae; Prosthecobacter.
REFERENCE   1  (bases 1 to 2993)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2993)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.30
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:34:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,742
            CDSs (total)                      :: 4,684
            Genes (coding)                    :: 4,676
            CDSs (with protein)               :: 4,676
            Genes (RNA)                       :: 58
            tRNAs                             :: 55
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2993
                     /organism="Prosthecobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12165_length_2993_cov_20.092920"
                     /isolate="AMR_MDS_5629"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1965333"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            31..2886
                     /gene="polA"
                     /locus_tag="PLP58_22225"
     CDS             31..2886
                     /gene="polA"
                     /locus_tag="PLP58_22225"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007413087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HRJ11314.1"
                     /translation="MSKRLFLLDGMALLYRAHFAFIKNPVRTSDGVNTSALYGFANTL
                     LDILKNQQPTHLAVVLDTAAPTPRHEIFPEYKAQREEMPEDIAGAIPQIHRLCAAMRI
                     PLLTRDGYEADDIIGILAKRSEGQGFETFMVTPDKDFGQLVSDSVRIYKPGRQGADVE
                     ILGVPEVCAKWGIERPEQVVDILALMGDAVDNIPGIPGFGEKTATALIQKYGSVENLI
                     ARAAELKGRQREKVEQHADKARLSRQLATIMHDAPLEVELEDLAVQGHDDEALKALFT
                     EFEFNALGKRLFGEEFKAGRGRALAAGQASSLTPVAGASSPGASAGPSDDLFSTTEIQ
                     QQSANPTRTLADTPHTYHAVRTAEERAALIGKLAELEGFCFDLETTSLDPRQTEVVGI
                     AFSWQAHEGWFVFFPRAAAEAAAVLEEFRAVFTKPGVEKTGHNLKFDLAVLLAKGLEV
                     AGPFFDTMLAHSLIEPEQRHGMDYLAESLLGYTPVPLSALIGLEKNDLFAQATMADVA
                     ESEPQKITDYAAEDADVTWQLASKLRPQLPPMGAEQVFYEIESPLLPVLTRMEHAGVK
                     VDVEVLREIGQTLEKRAGELHQSIQESAGMAFNLNSPKQLGEVLFDKLRLLDKPKKTA
                     TGQYQTNEQVLLSLTGAHPIIQDILDYREVTKLKNTYVDTLPNEVNRATGRIHTTFHQ
                     LMAATGRMASSNPNLQNIPIRSAHGREIRKAFVPGEPGWVLLSADYSQVELRVMAALS
                     EDPAMIEAFQNGLDIHQATAARVYGVPLDAVLPEMRRTAKMVNFGIIYGISAFGLAQR
                     LGIPRGEAGQIIENYFKQFPGVQKYTRQIVEDARRHGYVETITGRRRVVRDIDSANAT
                     IRGAAERVAMNTPIQGTAADMIKLAMIRIDAALRGSGLRTRMLLQVHDELLFEMPEAE
                     VEAARALILEHMRAALPLGDVPVEVEAGTGMNWLEAH"
BASE COUNT          546 a          886 c         1117 g          444 t
ORIGIN      
        1 gggggggctg gctctttagg ctcggcgggg atgtccaaac gcctttttct cctcgacggg
       61 atggcgctgc tttaccgcgc gcatttcgcc ttcatcaaga acccggtccg cacgagcgac
      121 ggggtgaaca cttcggcgct ctacgggttt gcgaacacgc ttttggacat cctgaaaaac
      181 cagcagccca cgcatctggc ggtggtgctg gacacggcgg cgccgacgcc gcggcatgag
      241 attttcccgg aatacaaggc gcagcgggag gagatgccgg aggacatcgc gggcgccatc
      301 ccgcagatcc accgcctgtg cgcggcgatg cgcatcccgc tgctgacgcg ggacggctac
      361 gaggcggatg acatcatcgg catcctggcg aagcgctcgg aggggcaggg cttcgagacg
      421 ttcatggtga cgccggacaa ggacttcggc cagctcgtgt cggactcggt gcgcatttac
      481 aagccgggca ggcagggcgc ggatgtggag atcctgggcg tgccggaggt gtgcgcgaag
      541 tggggcatcg agcgcccgga gcaggtggtg gacatcctgg cgctgatggg tgacgcggtg
      601 gacaacatcc ctggcatccc cggcttcggc gagaagacgg cgacggcgct gatccagaag
      661 tatggcagcg tggaaaacct gatcgcccgc gcggcggagc tgaagggcag gcaaagggag
      721 aaggtggagc agcacgcgga caaggcgcgt ctgtcgaggc aactggcgac gatcatgcac
      781 gacgcgcctc tggaggtgga gctggaggac ctggcggtgc aaggccacga tgacgaggcg
      841 ctcaaggcgc tgttcacgga gttcgagttc aacgcgctgg ggaagaggct gttcggggag
      901 gagttcaagg cggggcgcgg cagggcgctg gcggcaggcc aggcttccag cctgacacct
      961 gtggccgggg cttccagccc cggcgcgagc gccggaccgt cagacgacct gttttccacc
     1021 accgagatcc agcagcaatc cgcgaacccg acgcgcacgc tggcggacac gccgcacacc
     1081 taccacgcgg tgcgcacggc ggaggagcgc gcggctttga tcgggaagct cgcggagctg
     1141 gagggcttct gctttgacct ggagacgacc tcgctggacc cgcgccagac ggaggtcgtg
     1201 ggcatcgcct tctcctggca ggcgcatgag gggtggttcg tgtttttccc gcgcgcggcg
     1261 gcggaggcgg cggcggtgct ggaggagttc cgcgcggttt tcaccaagcc gggtgtggag
     1321 aagacggggc ataacctgaa gttcgacctc gccgtgctgc tggcgaaggg gctggaggtg
     1381 gcggggccgt tttttgacac gatgctggcg cactcgctca tcgagccgga gcaaaggcat
     1441 ggcatggact atctggcgga gagcctgctg ggctacacgc cggtgccgct gagcgcgctc
     1501 atcgggctgg agaagaacga tctctttgcc caggcgacga tggccgacgt ggcggagagc
     1561 gagccgcaaa agatcaccga ctacgcggcg gaggacgcgg atgtgacctg gcagctcgcg
     1621 tcgaagctgc gcccgcaact gccgccgatg ggcgcggagc aggtgtttta tgagatcgag
     1681 tcgccgctgc tgccggtgct cacgcgcatg gagcatgccg gcgtgaaggt ggatgtggag
     1741 gtgctgcggg agatcggcca gactttggaa aagcgcgcgg gcgagctgca ccagagcatc
     1801 caggagagcg cgggcatggc cttcaacctg aacagcccga agcagctcgg ggaggtgctc
     1861 tttgacaagc tgcgcctgct ggacaagccg aagaagacgg ccaccggcca ataccagacc
     1921 aatgagcagg tgctgctctc gctgaccggc gcgcacccga tcatccagga catcctggac
     1981 taccgcgagg tgacgaagct gaagaacacc tatgtggaca cgctgccgaa cgaggtgaac
     2041 cgcgccacgg gccgcatcca cacgacgttt caccagctca tggcggccac ggggcgcatg
     2101 gcgtcgagca atccgaacct gcaaaacatc ccgatccgca gcgcgcacgg gcgggagata
     2161 cggaaggcct ttgtgcccgg cgagccgggc tgggtgctgc tgagcgcgga ctacagccag
     2221 gtggagctgc gcgtgatggc ggcgctgagc gaggacccgg cgatgatcga ggccttccaa
     2281 aacgggctgg acattcacca ggccacggcc gcgcgtgtct atggcgtgcc gctggacgcg
     2341 gtgctgccgg agatgcggcg cacggcgaag atggtgaact tcggcatcat ctacggcatc
     2401 agcgcttttg gtctggcgca aaggctgggc atcccgcgcg gggaggcggg gcagatcatc
     2461 gagaactact tcaagcaatt ccccggcgtg caaaaataca cgcgccagat cgtggaggac
     2521 gcgcgccgcc acggctatgt ggagacgatc acgggccggc ggcgcgtggt gcgggacatt
     2581 gacagcgcca acgccacgat ccgcggcgcg gcggagcggg tggcgatgaa cacgcccatc
     2641 cagggcacgg cggcggacat gatcaagctg gcgatgataa ggatagacgc ggcgctgcgc
     2701 gggtccgggc tgaggacgcg gatgctgctg caggtgcatg acgagctttt gttcgagatg
     2761 cccgaggcgg aggtggaggc ggcgcgcgcg ctcatcctgg agcacatgcg cgcggctttg
     2821 ccgctggggg atgtgcccgt ggaggtggag gcgggcacgg gcatgaactg gctggaggcg
     2881 cattgaagcc gcgccggctc aaagcatgtc ctcgcgctcg cgcagccact ccggccggtc
     2941 ataacgcgcc tcgacgagaa cccgcgcgcg ctcccgcgcc ggcccgggca ggg
//
LOCUS       DAOMYJ010000395         2980 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Prosthecobacter sp. isolate AMR_MDS_5629
            NODE_12222_length_2980_cov_19.973675, whole genome shotgun
            sequence.
ACCESSION   DAOMYJ010000395 DAOMYJ010000000
VERSION     DAOMYJ010000395.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867850
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prosthecobacter sp. (wastewater metagenome)
  ORGANISM  Prosthecobacter sp.
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Verrucomicrobiaceae; Prosthecobacter.
REFERENCE   1  (bases 1 to 2980)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2980)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.30
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:34:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,742
            CDSs (total)                      :: 4,684
            Genes (coding)                    :: 4,676
            CDSs (with protein)               :: 4,676
            Genes (RNA)                       :: 58
            tRNAs                             :: 55
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2980
                     /organism="Prosthecobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12222_length_2980_cov_19.973675"
                     /isolate="AMR_MDS_5629"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1965333"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(152..730)
                     /locus_tag="PLP58_22230"
     CDS             complement(152..730)
                     /locus_tag="PLP58_22230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009961357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DJ-1/PfpI family protein"
                     /protein_id="HRJ11315.1"
                     /translation="MSQPKGKVLIVVGDATETVDTLYPYYRLIEGGYQPVVTAPEKRK
                     YQMVLHEVKPGWTITKEWEGYSIDCDVPFAEVRPEEYAGIFFSGGRAPEYIREDENLL
                     NFTRWFWDNQRPMASVCHGVEIPARAGIVQGLRMATVAKCKFDLEVCGGIYVNEPCVI
                     DRHMYSGRTYHDSGHFVGPWIAALDAWVAANA"
     gene            843..1994
                     /locus_tag="PLP58_22235"
     CDS             843..1994
                     /locus_tag="PLP58_22235"
                     /inference="COORDINATES: protein motif:HMM:NF012742.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator"
                     /protein_id="HRJ11316.1"
                     /translation="MKRIPRVALLIETARSYTRDMLAGVRRYIAEHGPWSIFIELRAS
                     DSAPPVWLSKWDGDGLICRTFNETIARAVKAGGLPVVETRSSHLCPEFPFVGMDNSLI
                     GRTVAQHFLSRGYRNFAAYSLDTERFFEQRVQNFVAAVNEAGADCPVLPGAEPESVRD
                     WEKAQQRLIEWLESLPKPVGVYAANDQLGFRVLDACQRAGISVPEEVAVVGTENEETL
                     CSLASPPMTSLRLDGHTVGYLAARTLDRMMRGQKPERMETLVPPRGIVVRASSDDLVI
                     HDKLVQKAARLIREQVAAGVNVDQLCAALHISRSSLERRMKEALGRTPKEEIQRQRFR
                     LVERLLRDTDLTIEAIAAQTGFTHSHYLQAAFKKHHGIPPGQFRQQREA"
     gene            complement(2170..2697)
                     /locus_tag="PLP58_22240"
     CDS             complement(2170..2697)
                     /locus_tag="PLP58_22240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ11317.1"
                     /translation="MGELVAGFDSKRRGSFRSWLMTCLRNLTIKDHHARRSLKRGGGW
                     KFEPLDSEAVEREMLAIQAVHLEHGPACDLILARCIWRSVARRMEEGRSAERVRLIRD
                     LLPLVLFEKWPPPPFPTQQEMADKHDMTVVNLRGYFHHTLKGRVRKLFAEEILGPPDE
                     ADYLWHMLCRYGDAA"
BASE COUNT          508 a         1015 c          946 g          511 t
ORIGIN      
        1 cagatctagg gagaggaatt ggcggcggtt gggtttcatg atgggtgggt ctgggattcg
       61 ttggagtgaa gcctttaggc gacacgggag cgggtgttgt ctcgaaagtt cattgaacaa
      121 gctaaagctt gaactccaac ggggactgca actatgcatt cgcggcgacc cacgcatcca
      181 gcgcggcgat ccaggggccg acgaagtggc cgctgtcgtg gtaggtgcgt cctgagtaca
      241 tgtgccggtc aatgacacac ggctcgttga catagatgcc gccgcagact tcgagatcga
      301 acttgcactt cgccaccgtg gccatgcgca ggccctgcac gatgccggcg cgcgcgggaa
      361 tctccacgcc gtggcagacg ctggccatgg ggcgctggtt gtcccagaac caacgcgtga
      421 aattcaataa gttctcgtct tcacgaatgt actccggcgc gcggccgccg ctgaaaaaga
      481 tgcccgcata ttcctccggc ctcacctcgg cgaagggcac gtcgcaatca atgctgtagc
      541 cctcccactc cttggtgatg gtccagccgg gtttgacctc gtgcagcacc atctggtatt
      601 tgcgtttttc cggcgcggtg acgacgggct ggtagccgcc ctcgatgagg cggtagtagg
      661 ggtaaagcgt gtccacggtt tccgtggcgt cgccgacgac gatgaggact tttcctttgg
      721 gctgtgacat gagagcgggt ctgggttgaa ggcgggcagc gccgccggga gagggatatt
      781 aaacgaggaa gggcttgcct ttctctgcat ttgtcgcgca tcatcctcag attttccgtc
      841 agatgaaacg catcccccga gtcgccctgc tcattgaaac cgcgcgcagc tacacgcgcg
      901 acatgctggc gggcgtgcgg cgctacatcg ccgagcacgg gccgtggtcc atcttcatcg
      961 agctgcgcgc ctcggactcc gcgccgccgg tttggctgtc caagtgggac ggtgacggtt
     1021 tgatctgccg gactttcaac gaaacgatcg cccgcgcggt gaaggccggg gggctgccgg
     1081 tggtggagac gcgctccagc cacctctgcc cggagttccc tttcgtgggc atggacaact
     1141 ccctcatcgg ccgcaccgtg gcccagcatt tcctctcgcg cggctaccgg aacttcgccg
     1201 cctacagcct cgacaccgag cgcttcttcg agcagcgggt gcaaaatttc gtcgccgctg
     1261 tcaacgaggc tggagcggac tgcccggtgc tccccggagc cgagccggag tccgtgcgcg
     1321 actgggaaaa ggcccagcag cgtctcattg aatggctgga gtccctgccc aagccggtgg
     1381 gcgtttatgc cgccaatgac cagctcggct tccgcgttct ggacgcctgc cagcgcgcag
     1441 gcatctccgt gccggaggag gtggccgtgg tgggcaccga aaacgaggag acgctctgct
     1501 ccctggccag cccgccgatg accagcctgc ggctcgacgg ccacaccgtg ggctacctgg
     1561 ccgcacgcac gctggaccgc atgatgcgcg ggcagaagcc cgagcgcatg gagaccctgg
     1621 tgccgccgcg cggcatcgtc gtgcgcgcct cctcggacga cctcgtcatc cacgacaagc
     1681 tcgtgcagaa agccgcgcgc ctcatccgcg agcaggtggc cgccggagtc aatgtggacc
     1741 agctctgcgc agcgctccac atctcccgca gcagcctgga gcggcgcatg aaggaggcgc
     1801 tggggcgcac gccgaaggag gagatccagc gccagcgctt ccgcctcgtc gagcgcctgc
     1861 tgcgagacac ggatctcacc atcgaggcca tcgccgcgca gaccggcttc acgcacagcc
     1921 actacctcca ggccgccttc aagaagcacc acggcatccc ccccggccag ttccgccagc
     1981 agcgtgaagc ctgacccggc ggccaggcgg tagccgccgc cgcaaggcgg tgggggattc
     2041 gaggatcggg caccggcttt gctgccggag tcgccacgcc cttgcgggcg cagccacgcc
     2101 gcagcagccg cctcaagacg gtggggctgg aaggatcggg cgcctgcttt gccgccaggc
     2161 ggcccttcgt tacgccgcat ccccataccg gcacagcatg tgccagagat agtccgcttc
     2221 gtcaggcggg ccgaggattt cctcggcaaa gagtttgcgc accctgccct tgagcgtgtg
     2281 gtggaagtag ccgcggagat tgaccactgt catgtcatgc ttgtccgcca tttcctgctg
     2341 ggttggaaaa ggcggcggcg gccatttttc gaaaagcacc aatggcagca ggtcccggat
     2401 caggcgcacg cgctccgcgc tccgcccctc ctccatgcgg cgcgccaccg agcgccagat
     2461 gcagcgcgcc aggatcaggt cgcaagccgg cccgtgctcg aggtgcacgg cctggatggc
     2521 gagcatctcg cgctccacag cctcggagtc cagaggctca aacttccagc ccccgccgcg
     2581 cttcagactg cgccgcgcat ggtggtcctt gatcgtcagg tttctgaggc aggtcatcag
     2641 ccagcttcgg aagctgccgc gcctcttcga gtcgaagccc gctacaagct cgcccattgc
     2701 cgccccataa ccagggcgtg gctgctgccg cccggcacat cgcccgcgtg ccggggaacc
     2761 tcccccgcca agtcctctgg gaattcggcg cagcatcgcc accaaagtcg aacacatcta
     2821 tgtgcgggaa ggctacggca tcagcctgcc cggcatctcc cccgcccgca tcagcgccgc
     2881 caccccccat gcctgatacc caccctcgag cgctggcagc acgccctgcc gcggaaatgc
     2941 caccctgctt tgtctccacg agcacacccg ccgcgccgcc
//
LOCUS       DAOMYJ010000396         2961 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Prosthecobacter sp. isolate AMR_MDS_5629
            NODE_12329_length_2961_cov_18.099449, whole genome shotgun
            sequence.
ACCESSION   DAOMYJ010000396 DAOMYJ010000000
VERSION     DAOMYJ010000396.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867850
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prosthecobacter sp. (wastewater metagenome)
  ORGANISM  Prosthecobacter sp.
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Verrucomicrobiaceae; Prosthecobacter.
REFERENCE   1  (bases 1 to 2961)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2961)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.30
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:34:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,742
            CDSs (total)                      :: 4,684
            Genes (coding)                    :: 4,676
            CDSs (with protein)               :: 4,676
            Genes (RNA)                       :: 58
            tRNAs                             :: 55
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2961
                     /organism="Prosthecobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12329_length_2961_cov_18.099449"
                     /isolate="AMR_MDS_5629"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1965333"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            145..1086
                     /locus_tag="PLP58_22245"
     CDS             145..1086
                     /locus_tag="PLP58_22245"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012419172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004070 - aspartate carbamoyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0016597 - amino acid binding [Evidence
                     IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase catalytic
                     subunit"
                     /protein_id="HRJ11318.1"
                     /translation="MSLKTPRKDLLDIASLSDEEIEFVLANAVPFKDLFKRSVKKVPT
                     LKGRTVLTLFYEPSTRTRSSFEVAANRLSADVTHFDIESSSVVKGESVLDTVETLESM
                     RADYIVVRHKQSGTPNLIAKNTRASVINAGDGWHAHPTQALLDAFTLREKLRDLRGAR
                     ALIVGDIQHSRVARSTSLILRRLGMSLAYLAPGSMMPRETPDEVGLFGNWDDALSWRP
                     DVVYLLRVQSERLDEPFFPTAAEYHKHYGLTDARVGILRERGLWLMHPGPVNRGVEIT
                     DTGMNYERSLINAQVENGIAVRMSVLYWLRPGALEES"
     gene            1090..2082
                     /locus_tag="PLP58_22250"
     CDS             1090..2082
                     /locus_tag="PLP58_22250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009962437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="KpsF/GutQ family sugar-phosphate isomerase"
                     /protein_id="HRJ11319.1"
                     /translation="METPAAIYDAPERARRVIRLEIEQLERLHERIGAAFSRAVEMLH
                     ACLSARHKLIVCGVGKSGNIGRKLAATLNSTGATAVCLDAGDALHGDLGVIDPGDLGI
                     VLSHSGETRELLDILPHLKRLGLPLIAITGDTGSTLARHAECVLDVHVTQEACPLNLA
                     PTASTTTMLVLCDALAMVLLEARGFRQEDFASLHPGGSLGRALLTRVADVMRSGPQLA
                     LIPPASSVQEALQAMTRARSGAAVVVSPEGALAGVFTHGDFVRAYQKDHDIASRPVSD
                     FMTARPITLEAGKLAAEVLTTLQNNRVDDIVVVDEAGRPVGLVDTQDLTRLRLV"
     gene            2131..>2961
                     /locus_tag="PLP58_22255"
     CDS             2131..>2961
                     /locus_tag="PLP58_22255"
                     /inference="COORDINATES: protein motif:HMM:NF012576.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HRJ11320.1"
                     /translation="MPGPTLSQIAEKTGVSKMTVSRALRGERHVADEVRERVRAAAAE
                     MHWRPDPEISKLMTHMRRGRRAAAPPALAFVWSEKVEKPPSPWSRRLFEGALERARLH
                     GYDLEEFQIGARGMTGRRLSDVLEARGIRGLVLSPLVSRSRGHVSLRWEKFSSVVIGL
                     GYARPELHRVHHHHFHGMMTVLRMLKKQKVRRAGFYSESTVNERMFGAWAASFLTHHP
                     LPAAQAARLLRLTRKPGREDFINWVLETRPEVVVDSGLRCAEWLAALPADSRPAHVTL
                     N"
BASE COUNT          478 a         1058 c          964 g          461 t
ORIGIN      
        1 cgcgccgggc gggaactacc gctctacccg cgctactcgg gcatcgagac cgacctgccg
       61 gccggcgagc gcgtgtgcgt gcgcttcgtg gatggcgacg gcgtggacga agtcttcacc
      121 tgcccctggg agaaaaagtg acacatgagc ctcaagacac cgcgcaaaga cctcctcgac
      181 atcgcctcgc tctcggatga ggagatcgaa ttcgtgctcg ccaacgccgt gccgttcaag
      241 gacctcttca agcggagcgt gaagaaggtg cccacgctca agggccgcac ggtgctcact
      301 cttttctatg agccgagcac gcgcacgcgc tcctccttcg aagtggccgc caaccgcctc
      361 tccgccgatg tcacccactt tgacatcgag tcctccagcg tggtcaaagg cgagtccgtg
      421 ctcgacaccg tggagacgct ggagtccatg cgcgcggact acatcgtggt ccgccacaag
      481 cagtcaggca cgccgaacct catcgccaaa aacacccgcg ccagtgtcat caatgccggt
      541 gacggctggc acgcccaccc gacccaggcg ctgctggacg ccttcaccct ccgggaaaaa
      601 ttgcgcgacc tgcgcggcgc gcgcgccctc attgtcgggg acatccagca ctcacgcgtg
      661 gcgcgcagca ccagcctcat cctgcgccgc ctcggcatga gcctggccta cctggcgccc
      721 ggctccatga tgccgcgcga gacgccggac gaggtgggcc tctttggcaa ctgggatgac
      781 gccctctcct ggcggcccga cgtggtctat ctgctgcgcg tgcagagcga gcggctcgac
      841 gagcccttct tccccaccgc cgccgagtat cacaagcact acggcctgac cgacgcgcgc
      901 gtcggcatcc tgcgcgagcg cggcctctgg ctcatgcacc ccggccccgt caatcgcggc
      961 gtcgagatca ccgacaccgg catgaattac gagcgcagcc tcatcaacgc ccaggtcgaa
     1021 aacggcatcg ccgtgcgcat gagcgtgctt tactggctgc ggccgggcgc tttggaggaa
     1081 tcctgagaca tggaaacccc cgccgccatc tacgacgcgc ctgagcgcgc ccgccgcgtc
     1141 atccgcctgg agatcgagca gctcgagcgc cttcatgagc gcatcggcgc ggccttttcc
     1201 cgggcggtgg aaatgctcca cgcctgcctc agcgcgcggc acaagctcat cgtctgcggc
     1261 gtcggcaaaa gcggcaacat cggccgcaag ctcgccgcca ccctcaacag caccggcgcc
     1321 accgccgtct gcctggacgc gggggacgcc ctgcatggcg acctcggtgt cattgaccca
     1381 ggcgatctcg gcatcgtcct cagccacagc ggcgagacgc gcgagctgct cgacatcctc
     1441 ccccacctca agcgcctcgg cctgccgctc atcgccatca cgggagacac cggctccacc
     1501 ctcgcccgcc acgccgagtg tgtgcttgat gtccatgtca cccaggaggc ctgcccgctg
     1561 aatctggccc ccaccgccag caccaccacc atgctcgtgc tgtgcgacgc gctggccatg
     1621 gtgctgctgg aggcgcgcgg cttccgccag gaggacttcg ccagcctgca ccccggcggc
     1681 tccctcggcc gcgccctgct cacccgtgtc gccgatgtca tgcgcagcgg tccgcagctc
     1741 gccctcatcc cgcccgcgtc cagcgtccag gaggcccttc aggccatgac ccgcgcccgc
     1801 agcggcgcgg cggttgttgt ctcaccggag ggtgcgctcg cgggcgtctt cacgcatggc
     1861 gactttgtcc gcgcctatca aaaggatcat gacattgcca gccgcccggt cagcgatttc
     1921 atgaccgcgc gcccgatcac gctggaggcc ggcaaactcg ccgccgaggt cctcaccacc
     1981 cttcaaaaca accgcgtgga cgacatcgtc gtcgtggacg aggcaggccg cccggttggc
     2041 cttgtggaca cccaggacct cacgcggctg cgccttgtgt aggcatcccc gccatgtgga
     2101 ggcgcgcgca ttcccagata ctcctccgcc atgcccggac ccacgctaag ccagattgcc
     2161 gaaaagaccg gagtgtcgaa gatgaccgtc tcgcgcgctc tgcgcgggga gcggcatgtg
     2221 gcggacgagg tgcgggagcg ggtgcgcgcg gcggcggcgg agatgcattg gcggccggac
     2281 ccggaaattt cgaagctgat gacgcacatg cggcgcggca ggcgcgcggc ggctccgccg
     2341 gcgctggctt ttgtgtggtc ggaaaaagtg gaaaagccgc cctcgccctg gtcgcggcgg
     2401 cttttcgaag gggcgctgga aagggcgcgg ctgcacgggt atgacctgga ggagttccag
     2461 atcggggcgc gcggcatgac ggggcggcgg ctctcggacg tgctggaggc gcgcggcatc
     2521 cgcgggctgg tgctctcgcc gctcgtctcg cgcagccgcg gccatgtgtc cctgcgctgg
     2581 gagaaattca gcagcgtggt catcggcctg ggttatgcgc gccccgaact gcaccgggtg
     2641 catcaccacc acttccacgg catgatgacg gtgctgcgaa tgctgaaaaa gcagaaggtc
     2701 aggcgcgccg gcttttactc ggagagcacg gtgaacgagc gcatgttcgg cgcgtgggcg
     2761 gcgagtttcc tgacgcatca cccgctgccg gcggcgcagg ctgcgcggct gctgcggctg
     2821 acgcgcaagc cggggcgcga ggatttcatc aactgggtgc tggagacgcg cccggaggtg
     2881 gtggtggaca gcggcctgcg ctgcgcggaa tggctggccg ccctgccggc ggactcgcgg
     2941 cccgcgcatg tgacgctgaa c
//
LOCUS       DAOMYJ010000397         2955 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Prosthecobacter sp. isolate AMR_MDS_5629
            NODE_12369_length_2955_cov_19.758276, whole genome shotgun
            sequence.
ACCESSION   DAOMYJ010000397 DAOMYJ010000000
VERSION     DAOMYJ010000397.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867850
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prosthecobacter sp. (wastewater metagenome)
  ORGANISM  Prosthecobacter sp.
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Verrucomicrobiaceae; Prosthecobacter.
REFERENCE   1  (bases 1 to 2955)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2955)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.30
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:34:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,742
            CDSs (total)                      :: 4,684
            Genes (coding)                    :: 4,676
            CDSs (with protein)               :: 4,676
            Genes (RNA)                       :: 58
            tRNAs                             :: 55
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2955
                     /organism="Prosthecobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12369_length_2955_cov_19.758276"
                     /isolate="AMR_MDS_5629"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1965333"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(14..142)
                     /locus_tag="PLP58_22260"
     CDS             complement(14..142)
                     /locus_tag="PLP58_22260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ11321.1"
                     /translation="MKRLFRWSILAAAIALPLAAALVWGCDAWLGVPESEGVHARR"
     gene            855..1478
                     /gene="gmk"
                     /locus_tag="PLP58_22265"
     CDS             855..1478
                     /gene="gmk"
                     /locus_tag="PLP58_22265"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012420526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004385 - guanylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HRJ11322.1"
                     /translation="MHSHQPRLGLLVIVSGPSGTGKTTLCRQVCDGVRAVFSVSCTTR
                     APRPGEVDGRDYFFLSEDDFLARVDRGELFEHARVHGRWYGTLKSHVFDNLRRGVDVF
                     MDIDVQGAAQVRACQDELVRRCLTDIFVMPPGIGELRARLLGRATDSAEVVELRLRNA
                     EAELRHWREYRYNLVSGERPRDEARFKAILEAERMRSSLVACGGDAW"
     gene            complement(1494..2699)
                     /locus_tag="PLP58_22270"
     CDS             complement(1494..2699)
                     /locus_tag="PLP58_22270"
                     /EC_number="2.3.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009960896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine O-acetyltransferase"
                     /protein_id="HRJ11323.1"
                     /translation="MSQGLGATATQFFHLGSADEPFEFASGERLPGITVAYETYGKLN
                     DKGSNAILLFHALSGSQHAAGVNTAIEGVGARWTEDVHLGWWDLFIGPGKALDTDRYF
                     VICANYLGGCYGSSGPLSINPATGMPYGRDFPPVLSSDIVRSQAALLDHLGIRALHAI
                     IGASVGGLLAINFATLFPDRVRIVIPIATGLSTTVLTRLTLFEQVLAIENDPHFNGGD
                     YTCERSPEYGLALARMISHKTFVHLDAIERRARQDVSQPNGQLAWYKVGHNVESYMLH
                     QGKKFVKRFDANTYLRIINMWLRYDPLADAGVGGFAELFQRSKAAGHHYLVFSIDSDF
                     CFYPEEQSLLVAELEKAGVSSMYITVHSDKGHDSFLLEPELYTPHLVYTLEGRWGKDS
                     RRSHVAQVE"
BASE COUNT          526 a          878 c         1052 g          499 t
ORIGIN      
        1 cttcgcgccc aattcaccgg cgcgcatgca cgccttcgct ttccggaact ccgagccagg
       61 catcgcaccc ccagacgagc gcggcggcca ggggcagcgc gatggcggcg gcgaggatgc
      121 tccagcggaa aagtcgcttc atgacgtgaa aaaccacccg gctggcccga ctgcaagccg
      181 caagtgaggc ggcgggaggc cgcgagccgc ctcccttccc ctgtcccagc gttctcgttc
      241 cttcgaatgg ctgcgccaag gtgcgaaagg ccgggttcat ctgttcaaat gccgcaatca
      301 ccaggaagac tgcggcaatc gaacagtcgt ccgcaccaga cgaacggtag attgagccag
      361 aagaacggta gttcgggcca aactaacggt agtttgcgcc agacgaacgg tagtttggac
      421 caaactaacg gtagtttgcg ccagacgaac ggtagtttgg accgaacgaa tggtagtttg
      481 cgccggacga acggtagttt gaaccaaacg aatggtagtt tgcgccagac gaacggtagt
      541 ttggaccaaa cgaacggtag tttgcgccag acgaacggta gtttggacca gacgaacggt
      601 agtttgcgcc agacgaacgg tagtttgaac caaacgaatg gcagcttgga gcgggtgaac
      661 ggtagtccgg gccaaaatat cggtggtttg cgccagcagg acggcagtct ggctcgaacg
      721 acatgcagtt tgacggagcg ctcctgcccg gcgttggcgg cggttgagcc ttgcgctgct
      781 ggcgcggggt gggccgggct gcggcggcgc atttccacct tgcggcgcgg tggcgttgtc
      841 gctaaacgct gcccatgcac tcccaccagc cacgccttgg acttctggtc attgtctccg
      901 gcccctccgg cacggggaag accacgctct gccgccaggt gtgcgacggg gtgcgggcgg
      961 tgttttccgt gtcatgcacg acgcgcgcgc cgcgccccgg cgaggtggac gggcgcgact
     1021 attttttcct cagcgaggac gatttcctgg cccgcgtgga ccgcggcgag ctcttcgagc
     1081 acgcgcgcgt gcacggccgc tggtatggca cgctgaagag ccatgtcttc gacaatctgc
     1141 ggcgcggggt ggatgtgttc atggacattg acgtgcaggg cgcggcgcag gtgcgcgcct
     1201 gccaggatga gctggtgcgc cgctgcctga cggacatatt tgtcatgccg ccgggcatcg
     1261 gagagctgcg cgcgcggctg ctgggccgcg ccacggacag cgccgaggtg gtggaactgc
     1321 ggctgaggaa cgccgaggcg gagctgcgcc actggcgcga gtaccgctac aatctcgtca
     1381 gcggcgagcg gccgcgggac gaggcgcgct tcaaagccat cctggaggcg gagcggatgc
     1441 gctccagcct ggtggcgtgc ggcggcgacg catggtgatc aaggccgcgc gcgtcactcc
     1501 acctgcgcca catgggagcg gcgggagtcc ttgccccagc ggccctcaag cgtgtacacg
     1561 agatgcgggg tgtagagctc gggctcgagc aggaaggagt cgtgcccttt gtcggaatgc
     1621 acggtgatgt acatgctgga gacgccggct ttctccagct cggcgaccag cagggactgc
     1681 tcctcggggt aaaaacaaaa gtccgagtca atgctgaaga ccaggtagtg atgcccggcg
     1741 gctttgctgc gctggaaaag ctccgcaaag ccgccgacgc cggcgtcggc gagcgggtcg
     1801 tagcgcagcc acatgttgat gatgcgcagg taggtgttgg cgtcgaagcg cttcacgaac
     1861 ttcttgccct ggtggagcat gtagctctcc acgttgtggc ccactttgta ccaggccagt
     1921 tggccgttcg gctggctcac atcctggcgg gcgcggcgct cgatggcgtc gagatgcacg
     1981 aaggtcttgt gcgagatcat gcgcgccagg gccaggccgt attcggggct gcgctcgcag
     2041 gtgtagtcgc cgccgttgaa gtgcgggtcg ttttcaatgg ccagcacctg ctcgaacaaa
     2101 gtcagccgcg tcagcacggt ggtgctcagg ccggtggcga tggggatgac aatgcgcacc
     2161 cggtccggga agagggtggc gaaattgatg gccagcagcc cgcccactga cgcgccgatg
     2221 atggcgtgca gcgcgcggat gccgagatgg tcaagcagcg cggcctggct gcgcacgatg
     2281 tcgctggaga gcaccggggg gaaatccctg ccgtagggca tgcccgtggc cgggttgatg
     2341 gagagcggtc cgctggagcc gtagcagccg ccgaggtagt tcgcgcagat gacgaagtag
     2401 cggtccgtgt cgagagcctt gccggggccg atgaaaagat cccaccagcc gagatgcacg
     2461 tcctccgtcc agcgcgcgcc cacgccttcg atggcggtgt tcacgcccgc cgcgtgctgg
     2521 ctgccggaga gggcgtgaaa gagcaggatg gcgttgctgc ccttgtcatt gagtttgccg
     2581 taggtctcgt atgccacggt gatgccgggc agcctttcac cgctggcgaa ttcgaagggt
     2641 tcgtcagcgg agccgagatg gaaaaactgg gtggcggtcg cgccaaggcc ttgggacata
     2701 gggcgcgcag caaacagcag gatggggcgc gcgcaacccc ggcgggcatt ttccagcgcg
     2761 cgaaaaagac cccgctgctc agggcgcgac cgggccgatg agccgcctgc cgctgcggtc
     2821 tgtctccacg gcgatccagc cgtcgaaggt gacctcgaca tggccgtcct gcctgcgccc
     2881 ggtggcaaag acggggacgc cggggttgaa cacggccagc accggcgtgc cgcccgtgct
     2941 ggcgcgcttg aggga
//
LOCUS       DAOMYJ010000398         2948 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Prosthecobacter sp. isolate AMR_MDS_5629
            NODE_12423_length_2948_cov_15.683374, whole genome shotgun
            sequence.
ACCESSION   DAOMYJ010000398 DAOMYJ010000000
VERSION     DAOMYJ010000398.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867850
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prosthecobacter sp. (wastewater metagenome)
  ORGANISM  Prosthecobacter sp.
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Verrucomicrobiaceae; Prosthecobacter.
REFERENCE   1  (bases 1 to 2948)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2948)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.30
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:34:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,742
            CDSs (total)                      :: 4,684
            Genes (coding)                    :: 4,676
            CDSs (with protein)               :: 4,676
            Genes (RNA)                       :: 58
            tRNAs                             :: 55
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2948
                     /organism="Prosthecobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12423_length_2948_cov_15.683374"
                     /isolate="AMR_MDS_5629"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1965333"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(86..1513)
                     /gene="purB"
                     /locus_tag="PLP58_22275"
     CDS             complement(86..1513)
                     /gene="purB"
                     /locus_tag="PLP58_22275"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009960728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004018 - N6-(1,2-dicarboxyethyl)AMP
                     AMP-lyase (fumarate-forming) activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HRJ11324.1"
                     /translation="MIDAIPNVLAERYATPAMRALWSAEGKVRLERDYWIAVLKGQRE
                     LGIPVPEGVIEAYESVKDKVFLASIMQRERVTRHDVKARIEEFCELAGHEHIHKGMTS
                     RDLTENVEQLQVFRSLLEVRKKSLAALAAFARRAVEARDIPFTARTHNVAAQVTTLGR
                     RWAMFGEELLLGVQALEDLIRTYPARGLKGAVGTRLDQITLFNGDAAKARELETRILR
                     HLGISAALDSPGQVYPRSLDLQVVSALCQLASGPSSFARTLRLMAGHELAGEGFAKGQ
                     VGSSAMPHKMNNRSCERVNGLHVILKGHLAMAAGLAGDQWNEGDVSCSVVRRVMLPDA
                     FLAMDGLLETLLTILNQMEVFDSVVESELLRYLPFLLTTTVLMQAVKAGAGREAAHEA
                     IKEHAVAVAKDLRTGKTARNNLLDRLAADPRLGLDTAALESLLAQGRANAGDGPAQVD
                     HFHARVSALLAAHPDAAAYQPGSIL"
     gene            1701..2798
                     /gene="dnaN"
                     /locus_tag="PLP58_22280"
     CDS             1701..2798
                     /gene="dnaN"
                     /locus_tag="PLP58_22280"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009963080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HRJ11325.1"
                     /translation="MKFTISKESLQQAIQQVQHVVSARTTLAILSNVLLRARDGVLEL
                     TTTDLDVGVTCSVAAEVEEEGATTLPARKLATIVRELPAEEVEISVDGKNTASIRSGP
                     SFFKVHGLSSEEFPSLPGFDEAREFKIEQPLLRDCLRKTSYAISTDETRYVLNGILFS
                     FKGSGLTMVATDGRRLAMVEQEVEFPESQAVDIIVPTKAVNELARLLGDNGDVIIRVS
                     GSQVGFDLGESLLISKLIDGNYPNYRQVIPGEAKEKIVLEREAFLRAISRVSLLATDK
                     SNSVKFIFTDGQVEIVASSPDVGEARETLPINYKGADITIAFNPEFTMAPLRNLTADE
                     VTLHLIDEISPGVLRTGASFLYVLMPMRVNN"
BASE COUNT          496 a          901 c         1061 g          490 t
ORIGIN      
        1 gggagggcgg gtcttgggga ccggattgac ctaactgacc gggcggaacg ggcggacctg
       61 gacgagctgg ctggctcatg gcgggtcaaa ggatgctgcc gggctggtag gcggcggcgt
      121 cggggtgcgc ggcgagcagg gcgctgacac gggcgtgaaa gtggtccacc tgagcggggc
      181 cgtcgccggc attggcgcgt ccctgggcca ggaggctctc caatgcggcg gtgtcgagcc
      241 cgaggcgcgg gtcggcggcg aggcggtcga gcaggttgtt gcgtgcggtt ttgccggtgc
      301 gcaggtcttt ggcgacggcg acggcgtgct ccttgatggc ctcgtgcgcg gcctcgcgcc
      361 cggcgccggc tttgacggcc tgcatgagca cggtggtggt gagcaggaag ggcaggtagc
      421 gcagcagctc gctctcgacg acggagtcga agacttccat ttgattcaaa atggtgagca
      481 gggtctccag caggccgtcc atggcgagga aggcatccgg caacatgacg cggcggacga
      541 cggagcagga gacatcgccc tcgttccact ggtcgccggc gaggccggcg gccatggcga
      601 ggtggccttt gaggatgacg tggaggccgt tcacgcgctc gcaggagcgg ttgttcattt
      661 tgtgcggcat ggcggagctg ccgacctggc ctttggcgaa accctcgccg gccagctcgt
      721 gcccggccat gaggcgcagg gtgcgggcga agctggaggg gccggaggcg agctggcaga
      781 gggcggagac gacctggaga tccaggctgc gcggatagac ctggccgggg gagtcgagcg
      841 cggcggagat gccgaggtgg cggaggatgc gggtttcgag ttcgcgggcc ttggcggcgt
      901 cgccattaaa aagggtgatc tggtcgagcc gggtgccgac ggcgcctttg aggccgcgcg
      961 ccgggtaggt gcggatgagg tcctccaggg cctgcacgcc gaggagcagc tcctccccaa
     1021 acatggccca gcggcggccg agggtggtga cctgggcggc gacgttgtgc gtgcgcgcgg
     1081 tgaaggggat gtcccgcgcc tcgacggcgc ggcgggcgaa ggcggcgagg gcggcgaggc
     1141 tttttttccg cacttccagc agggagcgga agacctggag ctgctcgaca ttttcggtga
     1201 ggtcgcggct ggtcatgcct ttgtggatgt gctcgtgccc ggccagctcg cagaattcct
     1261 cgatgcgcgc cttcacatcg tggcgggtga cgcgctcgcg ctgcatgatg gaggcgagaa
     1321 agaccttgtc cttcacggac tcgtaagcct cgatgacgcc ttccggcacg gggatgccga
     1381 gctcgcgctg gcctttgagc acggcgatcc agtagtcgcg ctccaggcgg accttgccct
     1441 cggcggacca gagggcgcgc atggcggggg tggcgtagcg ttcggcgagg acattgggga
     1501 tggcgtcaat catggggcgg gcatggtggg cgcgggcagg cggtgaggaa agttgaaaaa
     1561 tggaatcccg ccgcgtgaga agggggctaa aaaagaatgg aatccagggt ttccgaccgg
     1621 gaatttccgc gccaaacttc ttgccacccg ctcccggtca gataaaaagg cggattgatc
     1681 ccaggcgcca gccccacttt atgaagttca ccatcagcaa ggaatctctc cagcaggcca
     1741 tccagcaagt gcagcacgtc gtcagcgcgc gcaccaccct ggccattctc tccaacgtcc
     1801 tgctgcgcgc gcgcgacggg gtgctggagc tgaccacgac cgacctggac gtgggcgtga
     1861 catgcagcgt ggccgccgag gtggaggagg aaggcgccac cacgctgccc gcgcgcaagc
     1921 tggcgacgat cgtgcgcgag ctgcccgccg aggaggtgga gatcagcgtg gacggcaaaa
     1981 acaccgcctc catccgcagc ggcccctcct ttttcaaagt ccacggcttg agcagcgagg
     2041 agtttccctc cctgcccggc ttcgacgagg cgcgcgagtt caaaatcgag cagccgctgc
     2101 tgcgcgactg cctgcgcaag acctcgtacg ccatctccac cgacgagacg cgttatgtgc
     2161 tcaacggcat tcttttctcc ttcaaaggca gcggcctgac catggtggcc accgacgggc
     2221 gcaggctggc gatggtggag caggaggtgg agttccccga aagccaggcg gtggacatca
     2281 tcgtgccgac caaggcggtc aacgagctgg cgcgcctgct cggcgacaac ggcgatgtga
     2341 tcatccgcgt cagcggcagc caggtgggtt ttgatctggg cgagagcctg cttatcagca
     2401 agctcattga cggcaactat ccgaactacc gccaggtcat ccccggcgag gcgaaggaaa
     2461 aaatcgtcct ggagcgcgag gcctttctgc gcgccatctc gcgcgtctcc ctgctcgcca
     2521 ccgacaagtc gaattctgtc aaattcatct tcaccgacgg gcaggtggaa atcgtcgcct
     2581 cctcgccgga tgtgggcgag gcgcgcgaga cgctgcccat caactacaag ggcgcggaca
     2641 tcaccatcgc cttcaacccc gagttcacca tggcgccgct gcggaatctg acggcggacg
     2701 aggtgacgct gcatctcatt gatgaaatca gccccggcgt gctgcgcacg ggggccagct
     2761 tcctttatgt gctcatgccc atgcgtgtga acaattaaca accgccgcgt catgctccgc
     2821 ccaactcatc tcgcccccct ggccgcgctc actgtcgtgc tcgccggttg ttacggtccg
     2881 gaaccccgcc ccttcgcggg ccgtggcggc cgctccctgg accgcccgcc gccgcgctat
     2941 ggcgagcc
//
LOCUS       DAOMYJ010000399         2937 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Prosthecobacter sp. isolate AMR_MDS_5629
            NODE_12481_length_2937_cov_23.705760, whole genome shotgun
            sequence.
ACCESSION   DAOMYJ010000399 DAOMYJ010000000
VERSION     DAOMYJ010000399.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867850
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prosthecobacter sp. (wastewater metagenome)
  ORGANISM  Prosthecobacter sp.
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Verrucomicrobiaceae; Prosthecobacter.
REFERENCE   1  (bases 1 to 2937)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2937)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.30
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:34:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,742
            CDSs (total)                      :: 4,684
            Genes (coding)                    :: 4,676
            CDSs (with protein)               :: 4,676
            Genes (RNA)                       :: 58
            tRNAs                             :: 55
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2937
                     /organism="Prosthecobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12481_length_2937_cov_23.705760"
                     /isolate="AMR_MDS_5629"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1965333"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..407)
                     /locus_tag="PLP58_22285"
     CDS             complement(<1..407)
                     /locus_tag="PLP58_22285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ11326.1"
                     /translation="MSRTFRKDKALQEDLRRLQSRAYGVADWAGDALRRYHACVPRLA
                     PEESRVLAPLHEWIFVPLTLWPFSITDLLSECISIVEKGKPLTARLRLIIELLPPVPH
                     EEVCAAVADHERQVQRGNYEDVVNTQAKFAQAEL"
     gene            685..1653
                     /gene="hprK"
                     /locus_tag="PLP58_22290"
     CDS             685..1653
                     /gene="hprK"
                     /locus_tag="PLP58_22290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009961214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HPr(Ser) kinase/phosphatase"
                     /protein_id="HRJ11327.1"
                     /translation="MSQPAKVKRPASITVEECFRVNEKALKLKLVGGDAGFGRKILEP
                     SVNRPGLALSGFFTYFAYKRVQVVGNSEHSYLDQLDPKTRLARFSQLCTWDIPCIVSA
                     RGRRLPDDLVQIANEAGICVFQTSMMTMKFINVATIKLEWAFAPTMLVHGCFVDVQGI
                     GVLIRGKSGSGKSEAVIGLLQRGGSLVADDAVRLRLIEDREIIGSSPEMTRNMIEIRG
                     LGILNVAALFGVGSVRMSKRLDLVVDLVHLKDAEELERVGMASQTENVMGLPVAKVEI
                     PVAPGRDVAGLIELAALNYKLRSFGYNSAVEFDQRLLKKIADEQIG"
     gene            1669..1941
                     /locus_tag="PLP58_22295"
     CDS             1669..1941
                     /locus_tag="PLP58_22295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009961215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HPr family phosphocarrier protein"
                     /protein_id="HRJ11328.1"
                     /translation="MTVSKELKICNKMGMHARPAAQFVKRASKYKCDVWVEKDEDPVN
                     GKSIMGLMMLAAGRGETIKLTTDGTDAEAAMADLEDLINSGFGDNE"
     gene            1981..>2937
                     /locus_tag="PLP58_22300"
     CDS             1981..>2937
                     /locus_tag="PLP58_22300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ11329.1"
                     /translation="MTRLPAFLALISCAALSARAQLPQAIVEAQRAKLLEGVESVPKT
                     GAPGPVAIWGQTAFPLLAAPDRAGAEIAVAAAAGFGKGRILLLGHNGYLEGAAGGGHA
                     RLMANCARWTANKEKPRIGLHGVRAHSFYEPLGSKVETLNGPLQARALSGLDLLVLNA
                     QGVTDAEQGAAILEWVKNGGGLIAGMTGWAFAQTSGGKDLAAAHGLNHALMEAGLAFT
                     DQGNMDAAAYAARTRLPALMNAADAVQALKRQRDKAATLSDAEALQAASAIQLAMSAQ
                     PPGRSQLQEAVLAALGGADARSRLPAPQSPLTQAAHAADRIRL"
BASE COUNT          538 a          958 c          941 g          500 t
ORIGIN      
        1 agctcagcct gggcaaactt ggcctgggtg ttcaccacgt cctcataatt gccgcgctgc
       61 acttggcgct catgatctgc cacagccgca cagacttcct catgcggcac aggcggcagc
      121 agctcgatga tcaagcgcag ccgcgccgtg agaggcttgc ctttttccac gatggaaatg
      181 cattcactca gcagatccgt gatgctgaag ggccacaagg tcagcggcac aaagatccat
      241 tcatgaaggg gtgccaagac gcgcgattcc tccggggcaa ggcgcggcac gcacgcatgg
      301 taacgccgca gcgcgtcgcc cgcccagtcg gccaccccgt aggcgcgtga ttggagacgg
      361 cgcaggtctt cttgcagcgc tttgtctttg cggaaggttc gactcatgcg ggaatgtggc
      421 tcatggcgcg ggctggcgca tcacccaatc caggcgctcg gtgccattca tctatccggg
      481 ggtggatttt gatggggcgc atggagttat gccgcgcgtc atcacgattt gtgattccca
      541 tcttggagtg ttggaattgt ggagtgatga atttccaatg ccccagcact ccatgactcc
      601 aagccggttg cacaatttgt ggtgacgcga ggcatatgat tttgtgcttg ggagcaggca
      661 ttccgctgct aagatcgccg ccgcatgagc cagcccgcga aagtcaaacg ccccgcctcc
      721 atcacggtgg aggagtgttt tcgcgtcaac gagaaggcgc tgaagctgaa gctggttggc
      781 ggtgatgcgg ggttcggaag gaaaattcta gagccctcgg tgaaccgtcc cggcctggcc
      841 ttgtccggtt ttttcaccta cttcgcctac aagcgcgtgc aggtggtggg aaactcggag
      901 cactcctatc tggatcagct cgacccgaaa acgcggctgg cacgcttcag ccagctctgc
      961 acctgggaca tcccgtgcat cgtgtccgcg cgcgggcgcc gcctgccgga cgacctggtg
     1021 cagatcgcca acgaggcggg aatctgcgtt ttccagacca gcatgatgac gatgaagttc
     1081 atcaacgtgg ccaccatcaa gctggagtgg gccttcgcgc cgacgatgct cgtgcatggc
     1141 tgcttcgtgg acgtgcaggg catcggcgtg ctcatccgcg gcaaaagcgg ctccggcaag
     1201 agcgaggcgg tgatcggcct gctccagcgc ggcggcagcc tggtggcgga cgacgcggtg
     1261 cggctgcgcc tcatcgagga ccgcgagatc atcggctcct ccccggagat gacgcggaac
     1321 atgatcgaga tccgcggcct cggcatcctc aatgtcgccg ccctgttcgg cgtcggctcc
     1381 gtgcgcatga gcaagcggct ggacctggtc gtggacctcg tgcatttgaa ggacgccgag
     1441 gagctggagc gcgtgggcat ggccagccag accgagaatg tcatgggact gcctgtggcg
     1501 aaggtggaaa tcccagtggc gccgggccgc gacgttgcgg gcctcatcga gctggcggcg
     1561 ctgaactaca agctgcgcag cttcggctac aacagcgccg tggagtttga tcaaaggttg
     1621 ttgaaaaaga tcgcggacga acagataggt tgaccccccg agacggacat gacagtcagc
     1681 aaagaactca aaatctgcaa caagatgggc atgcacgccc gtccggcggc gcagttcgtc
     1741 aagcgcgcca gcaagtacaa atgcgatgtc tgggtcgaga aggacgagga tcccgtcaac
     1801 ggcaagagca tcatgggact catgatgctg gcggcgggca ggggcgagac catcaagctc
     1861 accaccgacg gcaccgacgc cgaggccgcc atggccgacc tggaggatct gatcaactcc
     1921 ggcttcggcg acaacgagtg attcgcgcgt tccaggcgcg gtcttttctt tcctgccacc
     1981 atgacccgtc ttcccgcctt cctcgccctc atttcctgcg ccgccctcag cgcgcgcgcg
     2041 cagctcccgc aagccattgt cgaagcccag cgcgccaaac tgctcgaagg cgtcgagtct
     2101 gtgccgaaaa ccggggctcc ggggccggtc gccatctggg gtcagacggc cttcccattg
     2161 ctcgccgcgc cagaccgcgc gggcgcggag atcgcggtcg ccgctgcggc gggctttggc
     2221 aaaggccgca tccttttgct cggccacaac ggctaccttg agggcgcggc gggcggcggc
     2281 cacgcgcggt tgatggccaa ctgcgcgcgc tggacggcga acaaggaaaa gccgcgcatc
     2341 ggactgcacg gcgtgcgcgc gcactccttc tacgagcctc tcggctccaa agtcgaaacg
     2401 ctcaacggtc cgctccaagc gcgcgcgctg tccggtctcg accttctcgt gctcaatgcc
     2461 cagggcgtca ccgacgcgga gcagggcgcc gccattttgg aatgggtcaa aaacggcggc
     2521 ggcctcatcg ccggcatgac tggatgggcc ttcgcgcaga ccagcggcgg caaggacctg
     2581 gccgccgctc acggactcaa ccacgcgctc atggaggccg gcctggcctt cactgaccag
     2641 gggaacatgg acgccgccgc ttacgcggcg cgcacccggc tgcccgcgct catgaacgcc
     2701 gccgatgccg tccaggcatt gaagcgacag cgtgacaaag ccgccacgct ctccgacgcc
     2761 gaagccctgc aggccgccag cgccatccag ctagccatga gcgcgcagcc tccgggacgc
     2821 agccagctcc aggaggccgt cctcgccgcc cttggcggag cggatgcccg ctcgcgcctg
     2881 cctgcgccgc aaagccctct cacccaggcc gcgcacgccg ccgaccgcat ccgcctc
//
LOCUS       DAOMYJ010000400         2909 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Prosthecobacter sp. isolate AMR_MDS_5629
            NODE_12654_length_2909_cov_19.539944, whole genome shotgun
            sequence.
ACCESSION   DAOMYJ010000400 DAOMYJ010000000
VERSION     DAOMYJ010000400.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867850
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prosthecobacter sp. (wastewater metagenome)
  ORGANISM  Prosthecobacter sp.
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Verrucomicrobiaceae; Prosthecobacter.
REFERENCE   1  (bases 1 to 2909)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2909)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.30
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:34:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,742
            CDSs (total)                      :: 4,684
            Genes (coding)                    :: 4,676
            CDSs (with protein)               :: 4,676
            Genes (RNA)                       :: 58
            tRNAs                             :: 55
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2909
                     /organism="Prosthecobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12654_length_2909_cov_19.539944"
                     /isolate="AMR_MDS_5629"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1965333"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..492)
                     /locus_tag="PLP58_22305"
     CDS             complement(<1..492)
                     /locus_tag="PLP58_22305"
                     /inference="COORDINATES: protein motif:HMM:NF024846.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD/NAD(P)-binding protein"
                     /protein_id="HRJ11330.1"
                     /translation="MSHTLFTQVTIIGGGFSGVMTAVNLARLGLPSSQITILNTLRPA
                     GRGVAYGTRRMEHLLNVAARNMSAFPDLPDHFVNWLRSRSEFDLIRENELRERFIPRV
                     IYGDYLRSLMHHHLPDARLVDAEAVDYANRMVLLAGGGSIEAGRVVLATGNEAPADLP
                     GAES"
     gene            604..900
                     /locus_tag="PLP58_22310"
     CDS             604..900
                     /locus_tag="PLP58_22310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ11331.1"
                     /translation="MSLSSSPTRRSLIKSAGLAPVLNSIGTVAQTNGRPLIVDCQSHL
                     FFPEVLEMMRRRKTEPLVYEQGGTVFLKMGGWLRKVPEHYSSGQPSWPSWMRLA"
     gene            903..1703
                     /locus_tag="PLP58_22315"
     CDS             903..1703
                     /locus_tag="PLP58_22315"
                     /inference="COORDINATES: protein motif:HMM:NF016777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HRJ11332.1"
                     /translation="MTLLSTNDPGPEWFGNDGPAVARLIHDSLAEVVAAHPARFRGLC
                     VLPMQNESAAGAELDRCVKKLGFKGVLLYTNLAGAWCDEPQFHWLYARAEELGVPILL
                     HPAKPMTTDQVGGYELTSTLGNMFENTIALARIIASGLLDRHPRLKLVCPHLGGTLPY
                     ICGRMDHQLHVLKRGPQNLLRKPSEYLRSIYMDLVSPLPEAMRFALDFTSEERLLFSS
                     DHPWVQPGEILAPLRSLKLSAGTEARLLGEMRGSFSRFETPGLSRCAR"
     gene            complement(1734..>2909)
                     /locus_tag="PLP58_22320"
     CDS             complement(1734..>2909)
                     /locus_tag="PLP58_22320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007415672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1501 domain-containing protein"
                     /protein_id="HRJ11333.1"
                     /translation="IDTAVDGLKICQGLENIARVMDRATLIRSAVQPDLGSILHSRHQ
                     YHWHTGYVPPQTVASPHIGAWMAKVLGPRNQVMPAFINIGQRLEGVGESEELKAFTTA
                     GFFGSEFGPMNLPFPEEAAQSVRPPQGMDANRFANRDRLFRKLVDQNPNRDRMSDYQQ
                     QSMLRSMDNAYRLLSSKEREAFDITLEPKEVQAKYDTGRFGRGCLLARRLVEAGARFV
                     EVTTEYVPFLHWDTHKDGHQTVAKLHQEIDLPIATLIRDLEERGLLERTLVIIASEFS
                     RDAMIEGKPGSNANDQATFKVDTLGEMAHYGLHRHFTGGTSVVMFGGGMKKGHIHGQT
                     ADERPLIAIKDPVTVMDLHATIMTAMGISPKTEFTIEGRPFYVTEDGKGQPVQDVFA"
BASE COUNT          538 a          818 c          941 g          612 t
ORIGIN      
        1 tgattccgct cccggcaggt cggctggagc ctcatttcca gtggccagga cgacccgccc
       61 cgcctcgatg cttccgcctc cggccagcag caccatcctg ttcgcgtagt ccaccgcctc
      121 cgcgtccaca agccgtgcat ccggcagatg atggtgcatg agtgagcgca gatagtcgcc
      181 ataaatcacg cgcgggatga aacgctcgcg cagttcattt tcgcggatca gatcaaattc
      241 acttcgactg cgcagccagt tcacaaagtg atccggcaga tcaggaaagg cggacatgtt
      301 gcgggccgcc acattcagca ggtgctccat gcggcgagtg ccatacgcga ctccgcgccc
      361 ggccggacgg agcgtgttga ggatcgtgat ttgcgaggat ggcaggccga gccgagcgag
      421 attcacggct gtcatgactc cgctgaagcc gccgccaatg atcgtgactt gtgtgaaaag
      481 ggtgtggctc atttttctca aggccagaaa acgagttttt cgagaatgca caacgactta
      541 ttgcgaaatc gcaatatata tggtgcggca ggactcgcct ggtgaggacg tttccctgtc
      601 gccatgtcac tgtcatcctc ccccacacgg cgttcattga tcaagagcgc cggacttgct
      661 cccgtcttaa attcaatcgg aacagtcgca cagacgaatg gccggccctt gattgtggac
      721 tgccagagcc atttgttttt tcccgaagtg ctcgaaatga tgcggaggcg caaaacagag
      781 ccgctggtct atgagcaggg cggcacggtg ttcctaaaaa tgggaggctg gctgcgcaag
      841 gtaccggagc actattcaag cggccagcca agctggccgt catggatgcg gctggcatag
      901 agatgaccct gctcagcacc aatgatcccg gaccggagtg gtttggaaat gacgggccgg
      961 ctgtcgcccg tctcattcac gactcgctcg ctgaggtcgt cgcagcgcat ccggcgcgtt
     1021 tccgcgggtt gtgcgtgctg cccatgcaaa acgagagcgc ggccggcgcg gagctggatc
     1081 gttgcgtcaa aaagctcggc ttcaaaggcg tgctcctcta caccaatctt gcgggcgcct
     1141 ggtgtgatga gccgcaattt cactggctgt atgcccgcgc cgaggaactc ggcgtgccaa
     1201 tcctgctgca tcccgccaaa ccgatgacca cggatcaagt gggcggctac gagctcacca
     1261 gcacacttgg caacatgttc gaaaacacca tcgcgctggc ccgcatcatc gccagcggac
     1321 tgctcgacag gcatccaagg ctgaaactgg tctgtccgca tcttggaggc acgctcccct
     1381 acatctgcgg acgcatggac caccagcttc atgtcctgaa acgcgggccg cagaaccttt
     1441 tgagaaagcc aagcgagtat ttgcgctcga tttacatgga cctcgtctcg ccgctgccgg
     1501 aggcgatgag gtttgccctc gatttcacca gcgaagaacg gctgcttttt tccagcgatc
     1561 atccctgggt tcaaccgggg gaaatcctag ctccactgcg cagtctcaaa ctttcagcgg
     1621 gtaccgaggc cagactgctt ggcgaaatgc gcgggagctt ttccagattt gagacgccag
     1681 ggttgtcgcg ctgcgccaga tgatgtcatt cattgatcaa aatgatgtgt gtttcacgcg
     1741 aaaacatcct gcaccggctg gcctttgccg tcctcggtga cgtagaaggg gcggccttcg
     1801 atggtgaact cggtcttggg actgatgccc atggcggtca tgatcgtggc gtgcaggtcc
     1861 atgacggtga cggggtcttt aatcgcgatg agcgggcgct cgtcggcggt ctggccgtgg
     1921 atgtggccct ttttcatgcc gccgccaaac atcaccacgc tggtgccgcc ggtgaagtgg
     1981 cggtgcaggc cgtagtgagc catctcgccg agggtgtcca ctttgaaggt ggcctggtcg
     2041 ttggcgttgg agccgggttt gccttcgatc atggcgtcgc ggctgaactc gctggcgatg
     2101 atgacgaggg tgcgttcaag taggccgcgc tcttcgaggt cgcggatgag ggtggcgatg
     2161 gggaggtcga tttcctggtg gagtttggcg acggtttggt ggccgtcctt gtgggtgtcc
     2221 cagtgcagga aggggacata ttcggtggtg acctcgacaa aacgcgcgcc ggcttccacg
     2281 aggcggcgag cgagcaggca gccgcggccg aagcgaccgg tgtcgtattt ggcctgcacc
     2341 tctttgggtt cgagggtgat gtcgaacgcc tcgcgctcct tgctgctgag caggcggtag
     2401 gcgttgtcca tgctgcggag catggactgc tgctggtagt cgctcatgcg gtcgcggttc
     2461 gggttttggt ccacgagctt gcggaagagt cggtcgcgat tggcgaagcg gttcgcgtcc
     2521 atgccctgcg gcgggcgcac ggattgggcg gcctcttcgg ggaagggcag gttcatgggg
     2581 ccgaactcgg agccgaagaa gccggcggtg gtgaaggcct tcaattcctc gctctcgccg
     2641 acaccttcaa ggcgttggcc gatgttgatg aaagcgggca tgacttgatt gcgtggacca
     2701 aggaccttgg ccatccaggc gccaatgtgc gggctggcga cagtttgcgg tgggacgtaa
     2761 ccggtgtgcc aatggtactg gtggcgggaa tgtagaatgc tgccgaggtc aggctgcacg
     2821 gcgctgcgaa tgagtgtggc acggtccatg actcgcgcga tgttttcgag gccctggcag
     2881 attttcaggc cgtccaccgc ggtatcaat
//
LOCUS       DAOMYJ010000401         2890 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Prosthecobacter sp. isolate AMR_MDS_5629
            NODE_12770_length_2890_cov_19.792945, whole genome shotgun
            sequence.
ACCESSION   DAOMYJ010000401 DAOMYJ010000000
VERSION     DAOMYJ010000401.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867850
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prosthecobacter sp. (wastewater metagenome)
  ORGANISM  Prosthecobacter sp.
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Verrucomicrobiaceae; Prosthecobacter.
REFERENCE   1  (bases 1 to 2890)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2890)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.30
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:34:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,742
            CDSs (total)                      :: 4,684
            Genes (coding)                    :: 4,676
            CDSs (with protein)               :: 4,676
            Genes (RNA)                       :: 58
            tRNAs                             :: 55
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2890
                     /organism="Prosthecobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12770_length_2890_cov_19.792945"
                     /isolate="AMR_MDS_5629"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1965333"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(33..146)
                     /locus_tag="PLP58_22325"
     CDS             complement(33..146)
                     /locus_tag="PLP58_22325"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: protein motif:HMM:NF014033.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008817 - corrinoid adenosyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_process: GO:0009235 - cobalamin metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP:cob(I)alamin adenosyltransferase"
                     /protein_id="HRJ11334.1"
                     /translation="MSIATRRGDQGETDLLFGCRLPKSHPRVHALGAVTGP"
     gene            complement(182..1627)
                     /locus_tag="PLP58_22330"
     CDS             complement(182..1627)
                     /locus_tag="PLP58_22330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ11335.1"
                     /translation="MNTHRLFFLSALLCLTPGLLEAARLHVGAATVDITPEQPVGLDG
                     QRGIRISKKPETPIQAAALALEAREGDRVLDQAVIVSCDLVAIRDGVIEQVREKLAGR
                     AAGLDPEKVFLCATHTHTAPVTTDGKYALPEDGSVMRPSEYTAWMTARVADGIVEAWE
                     KRAPGRVAWGQSQAVIAQNRRPYYADGTAVMYGNPDSPNFRGIEGHEDHSVDVLFFWN
                     EREEMVATAVNVPSPAQEVGGGRSIHADFWHPVRAALRERHGGQLHILAWTGAGGDVT
                     SRPVINRAAEERMRKLRGGLTRLEELARRIVTAWEECLEGARHDIREEVVFQHHVETL
                     RLPYRKVTILERSAAMTEAAKFKEDPAQRWNYLWNQRVVDRFNAQKEGAQEPYPMELH
                     ALRLGDCAIATNSFELYTDYGIQMKARSPGVQTFVIQLAGPGTYLPTERAARHGGYGA
                     VIQSSQIGPDGGQILVEETVRALKALWPEKK"
     gene            2006..2878
                     /locus_tag="PLP58_22335"
     CDS             2006..2878
                     /locus_tag="PLP58_22335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009963498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HRJ11336.1"
                     /translation="MQTRRAFLHHSALCLAGFGGGKMLAAAPGSRPLLRAGLMTDLHY
                     ADKEPTKTRFYREALAKLDEAVDHMNREGPAFVAELGDFIDQADSVETEIGWLRTVEA
                     RYAKLEMPRHYVMGNHCVGTLTKEEFAAHTQSAGGHAAFEAGGVTFLILDSCFRSDGA
                     PYARKNFHWQDANLPKGGLAWLESELGRAGGPVIVLAHQRLDEDKAHAVRNAAEARAL
                     LEKSGKVLAVFQGHSHKNDYQRIAGIHYTTLVAMVEGSGAENSGHTLLDIMADGSLRV
                     NGYRRQANRTLPRA"
BASE COUNT          468 a         1071 c          887 g          464 t
ORIGIN      
        1 aacaaccgat ggcatactgc tcggggagca tattagggtc cagtcaccgc gccgagggcg
       61 tggacgcgcg ggtgggactt gggcaggcgg cagccaaaga gcaggtcggt ctccccctgg
      121 tcgcctcgtc gggtggcaat ggacatgcct catgctacgc gcgagccgcg ggccgcgccg
      181 gtcatttttt ctccggccac agcgccttga gcgcgcgcac cgtctcctcc accaaaatct
      241 gccctccatc cgggccgatc tggctgctct ggatcaccgc gccgtagccg ccgtgccgcg
      301 ccgcgcgctc cgtgggcagg taggtgccgg ggccggcgag ctggatgaca aaagtctgca
      361 cccccggact gcgcgccttc atctggatgc cgtagtcggt gtaaagctcg aaactgttcg
      421 tggcgatggc gcagtccccc aggcgcagcg cgtgcagttc catcggataa ggctcctgcg
      481 ctccctcctt ctgcgcgttg aagcggtcca ccacccgttg gttccacagg tagttccagc
      541 gctgcgcagg atcttccttg aactttgccg cctcggtcat tgccgcagag cgctccagga
      601 tggtgacctt ccggtaaggc aggcgcagcg tctccacatg atgctggaaa accacctcct
      661 cgcggatgtc atgccgcgcg ccctccaggc actcctccca ggccgtgaca atgcgccgcg
      721 ccagctcctc cagccgcgtg aggccgccgc gcagctttct catgcgctcc tccgccgcgc
      781 ggttgatgac ggggcgggaa gtcacatccc cgcccgcgcc cgtccacgcc aggatgtgca
      841 gttgcccgcc atgcctctcc cgcaaagccg cgcgcaccgg atgccagaaa tccgcgtgga
      901 tggacctgcc gccgcccacc tcctgcgccg gcgagggcac attgacggcc gtggcgacca
      961 tctcctcccg ctcgttccag aaaaacagca cgtccacact gtgatcctca tgcccttcga
     1021 tgccgcggaa attcggcgag tccgggttcc cgtacatcac cgccgtgccg tccgcgtaat
     1081 aaggccgccg gttctgcgcg atgaccgcct ggctctgccc ccacgccacc cggcccggcg
     1141 cgcgtttttc ccacgcctcg acgattccat ccgcgacccg cgccgtcatc cacgccgtgt
     1201 actcggaggg cctcatcacg ctgccatcct ccggcagcgc gtatttgccg tccgtcgtca
     1261 ccggcgccgt gtgcgtgtgc gtcgcgcaca gaaagacctt ctccgggtcc agccctgccg
     1321 cgcgcccggc cagcttttcc cgcacctgct cgatgacccc gtcgcggatc gccaccagat
     1381 cgcacgagac aatgaccgcc tggtccagca cccggtcccc ttccctggcc tccagcgcca
     1441 gcgccgccgc ctggatcgga gtctccggct ttttcgagat gcggatgccc cgctgcccgt
     1501 ccagccccac cggctgctca ggcgtgatgt ccaccgtggc cgcgccaaca tgtaggcggg
     1561 cggcttcgag caggcccggc gtcaggcaca gcagggcgga gaggaaaaaa aggcggtgag
     1621 tgttcatggc agaaaaagcg gcaaaacgtg acgctcaaaa ctacgcgccg cgcccaccct
     1681 ccctctcatc cgcgccgcct ggctctcggc tgacgcaaag cgccccgcgc agacactccc
     1741 gcccgctctg cacgggcatg gagagccggc tgccctccaa tgacagaacg gcggttgccg
     1801 gattcattcc agcaactgcc ggaatcaccc cgtgaacttc cgtaatcgtt ccgtgtgctc
     1861 ctgacaggag aggtgtccgc ctcttgaaaa agcccccttg tcctccccgc ccggcaggcc
     1921 gggacctgtc cctgccagga attttccggg agatttttca gccatccgcc gcaagtccgg
     1981 gccgccggcg gcgctatccc ttcccatgca aacacgccgc gcctttctcc accacagcgc
     2041 cctctgcctg gccggattcg gcggcgggaa aatgctggcc gccgcgccgg gcagcaggcc
     2101 gctcctgcgc gccggcctga tgacggacct gcactacgcg gacaaggagc caacgaaaac
     2161 gcgcttttac cgcgaggccc tggcgaagct ggacgaggcc gtggaccaca tgaaccggga
     2221 ggggcccgcc ttcgtcgccg agctggggga tttcattgac caggcggact ccgtggagac
     2281 tgagatcggg tggctcagga ccgtggaggc gcgctacgcc aagctggaga tgccccgcca
     2341 ctatgtcatg ggcaaccact gcgtcggcac gctgaccaag gaggagttcg cggcgcacac
     2401 acagtctgcg ggcgggcatg ccgccttcga ggccggcggt gtcacctttc tcatcctcga
     2461 ctcctgcttc cgctccgacg gcgcgcccta cgcgcggaag aatttccact ggcaggacgc
     2521 caacctgccc aagggcgggc tggcctggct ggagtccgaa ctgggcaggg ccggcggccc
     2581 tgtgatcgtg ctcgcgcacc agcggctcga cgaggacaag gcgcacgccg tgcgcaatgc
     2641 cgccgaggcg cgcgcgctgc tggagaagtc cggcaaggtg ctcgccgtct tccagggcca
     2701 ctcgcacaag aacgactacc agcggatcgc cggcatccac tacaccacgc tcgtcgccat
     2761 ggtggagggc agcggcgcgg agaacagcgg ccacacgctg ctcgacatca tggccgacgg
     2821 ctccctgcgc gtgaacggct accgcaggca ggcaaaccgg accctgcccc gcgcctgaac
     2881 cgcccgcctc
//
LOCUS       DAOMYJ010000402         2885 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Prosthecobacter sp. isolate AMR_MDS_5629
            NODE_12808_length_2885_cov_18.599647, whole genome shotgun
            sequence.
ACCESSION   DAOMYJ010000402 DAOMYJ010000000
VERSION     DAOMYJ010000402.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867850
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prosthecobacter sp. (wastewater metagenome)
  ORGANISM  Prosthecobacter sp.
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Verrucomicrobiaceae; Prosthecobacter.
REFERENCE   1  (bases 1 to 2885)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2885)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.30
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:34:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,742
            CDSs (total)                      :: 4,684
            Genes (coding)                    :: 4,676
            CDSs (with protein)               :: 4,676
            Genes (RNA)                       :: 58
            tRNAs                             :: 55
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2885
                     /organism="Prosthecobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12808_length_2885_cov_18.599647"
                     /isolate="AMR_MDS_5629"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1965333"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..606
                     /locus_tag="PLP58_22340"
     CDS             <1..606
                     /locus_tag="PLP58_22340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ11337.1"
                     /translation="AAGEGARLVYARHSADDGRTFSEPQPLNEEKPGVCACCSLRAHL
                     AADGTLTVLYRGAPEPAARGMIQLTHGAGRTTLTPLDDWRVARCPMSSASLLPAGPSL
                     RAAWENDGQILTGLLGEPAAAAQKIGPGNAKHPALAQNAKGQTLIASVIGSGWAKAGT
                     LRWDLLEAQGTPADSGEGGKLPVWSHPAAYARPDGTFVILR"
     gene            658..2028
                     /locus_tag="PLP58_22345"
     CDS             658..2028
                     /locus_tag="PLP58_22345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ11338.1"
                     /translation="MHLLNITLSAGLAAASLANAAVPAFSGAEGFGAASAGGRGGRVI
                     EVTNLSDAGPGSFRAAIEAEGPRTVVFRTGGTIELMSEVVLTHPFITIAGQTAPGGGI
                     TLKTHPSNTRSALTIAKGCHDVIIRHLRSRPGPHDGFPPLKSGREADTSEVKDALQIL
                     GARRVIVDHCSFSWATDEVVSTFYDAQDITIQWCIIAEALRKSRPDQSLPGKGLLIGS
                     KGGQRISVHHNLMAHNVGRNPLIKAEGIVDVVNNVVLAPAIVAMAIDAELGHSPANFV
                     GNHVSAPAADGLANGLAVVGGGTFSLFVKDNLGPKRLRGGQPEELFVNATNQGRKWIT
                     PDRHAAPPITTHPASEVVEAVLADAGCLRPLRDAADERILADVRQNRPRTVDSPAQVG
                     GWPDLAPGTAPADRDHDGMPDEWETQHGLNPGDPADGRTDADGDGYTNLEEFLNGTDP
                     KRAEGK"
     gene            2076..2549
                     /locus_tag="PLP58_22350"
     CDS             2076..2549
                     /locus_tag="PLP58_22350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ11339.1"
                     /translation="MSGTKRERALLPAGFSRRLRLQGFRCGEESPRPLSGAEGPQGPE
                     GAQGNDGPQGPPFASAVVDDVFTVPPGQPATVGVWFDGTQVHFTFEIPRGENGSDGPS
                     GEVSQQQLDDAIAGTSPNSNAVATLGLSISDPPTAWEVQEVANKLDELIYALRRQ"
     gene            complement(2637..>2885)
                     /locus_tag="PLP58_22355"
     CDS             complement(2637..>2885)
                     /locus_tag="PLP58_22355"
                     /inference="COORDINATES: protein motif:HMM:NF019886.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor-like helix-turn-helix DNA-binding
                     protein"
                     /protein_id="HRJ11340.1"
                     /translation="EDEKALCQCVLRLLPELPAGDAELLRRVDLGGESPTEIAAAANE
                     RVNTLNVRLLRARRKLREQVERTCRACATHGCLDCDCP"
BASE COUNT          484 a         1013 c          987 g          401 t
ORIGIN      
        1 gcggcggggg agggcgcgcg cctcgtctat gcgcggcact ccgccgatga cggccgcacc
       61 ttttcagaac ctcagccgct caatgaggaa aagcccggcg tctgcgcctg ctgctccctg
      121 cgcgcgcatc tggccgcgga tggcacgctc accgtcctct accgcggcgc accggagccg
      181 gcagcgcgcg gcatgatcca gctcacccac ggggctggcc gcaccacctt gaccccgctg
      241 gatgactggc gcgtggccag gtgccccatg agcagcgcca gcctgctgcc cgccgggccg
      301 tccctgcgcg ccgcctggga aaacgacggc cagatcctca ccggactcct gggcgagccc
      361 gcggctgccg cccagaaaat cggccccggg aacgccaagc atcccgccct ggcgcagaat
      421 gccaaggggc agaccctcat cgccagcgtc atcggctccg gctgggccaa ggccggcacc
      481 ctgcgctggg acctgctgga ggcccagggc acccccgcgg attccggcga aggcggaaag
      541 ctccccgtct ggagccaccc cgccgcctac gcccggcccg atggcacctt cgtcatcctg
      601 aggtgacgcc atggcggcac accgcaacat gcctgctgcc aggccgttca taaggccatg
      661 cacctgttga atatcaccct gtcagcgggc ctggccgccg cttcgctggc gaatgccgca
      721 gtccccgcct tctctggcgc ggagggcttt ggagccgctt cggccggcgg ccgtggcggg
      781 cgtgtgatcg aggtgacgaa cctcagcgac gccggaccgg gcagtttccg cgctgccatt
      841 gaggccgagg ggccgcgcac cgtcgtgttc cgcaccggcg gcacgatcga gctcatgagc
      901 gaggtcgtgc tgacgcatcc cttcatcacc attgcgggac aaaccgcgcc gggcggcggc
      961 atcacgctga agacgcatcc ctcaaacacc cgctcggcgc tgaccatcgc caaaggctgc
     1021 cacgatgtca tcatccgcca cctgcgcagc cgtccggggc cgcatgacgg ctttccgccg
     1081 ctgaaaagcg gccgcgaggc ggacacgagc gaggtgaagg acgcgctgca gatcctcggc
     1141 gcccggcgcg tgatcgtgga ccactgctcc ttcagctggg ccacggacga ggtcgtttcg
     1201 accttctacg acgcgcagga catcacgatc cagtggtgca tcatcgccga ggcgctgcgc
     1261 aaaagccgcc cggatcagag cctgccgggc aaagggctgc tcatcggctc caaaggcggc
     1321 cagcgcatct ccgtgcatca caacctcatg gcgcacaatg tcggccgcaa tccgctcatc
     1381 aaagcggaag gcatcgtgga tgtggtgaac aacgtcgtgc tcgcgcccgc gatcgtggcg
     1441 atggccattg acgcggagct cggccactcg cccgcgaact tcgtcggcaa tcacgtgagc
     1501 gccccggcgg cggatgggct cgccaacggc ctcgcggtgg tcggcggcgg cacgttttcg
     1561 ctgttcgtga aggacaacct cggcccgaag cggcttcgcg gcggccagcc ggaggagctt
     1621 ttcgtcaatg ccaccaacca agggcggaaa tggatcacgc cagaccgcca cgccgcgccg
     1681 cccatcacca cgcatcccgc ctccgaggtg gtcgaggccg ttttggccga tgcaggctgt
     1741 ctgcgcccgc tgcgcgatgc cgcggacgag cgcatcctcg ccgacgtccg tcaaaacagg
     1801 ccgcgcaccg tcgattcacc cgcgcaggtc ggcggctggc ccgatctcgc gccaggaacc
     1861 gcccctgccg accgggatca cgacggcatg cccgacgaat gggagacgca gcacggtctc
     1921 aaccccggcg atcccgccga cggccgcacc gatgccgatg gcgacggcta cacgaacctg
     1981 gaggagtttc tcaacggcac cgacccgaag cgcgccgaag ggaaatgatg ccccctatcc
     2041 gaagcgtgcg ccctcattca tcctgcgccg aagcgatgag cggcacgaaa cgagagcggg
     2101 cactcctgcc cgccgggttc tccaggcggc tgcgcctcca gggtttcaga tgcggagagg
     2161 agtctccgcg ccccctttct ggcgcggaag gtccgcaggg gccggagggt gcccagggca
     2221 atgatggtcc gcaggggccg ccctttgcca gcgcggtggt ggacgatgtc ttcaccgtgc
     2281 cccccggcca gccggcgacg gtgggggtgt ggttcgatgg cacgcaggtg catttcacct
     2341 tcgagatccc gcgcggggag aatggcagcg atggcccctc gggcgaggtg agccagcagc
     2401 agctggacga cgccatcgcc gggacaagcc ccaacagcaa cgccgtagcc accctgggcc
     2461 tgagcatcag cgacccgccg accgcctggg aagtgcagga ggtggcgaac aagctggatg
     2521 agctgatcta cgcgctgcgg cggcagtgag gggggaatgg aggagacaat gagacaatga
     2581 gacaatgaga cggggagatg gggcgggagc ggtgagggga agaggcgcgg ctgtggctac
     2641 gggcagtcgc aatccaggca gccgtgggtg gcgcaggcgc ggcaggtgcg ctccacctgc
     2701 tcgcgcagtt tgcggcgggc gcgcaggagg cggacgttga gggtgttcac gcgctcgttg
     2761 gccgccgcgg cgatctccgt gggggattcg ccgccgagat ccacacggcg gagcagctcc
     2821 gcatcgcccg ctggcagctc gggcagcagc cgcagcacgc actggcagag ggctttctcg
     2881 tcctc
//
LOCUS       DAOMYJ010000403         2854 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Prosthecobacter sp. isolate AMR_MDS_5629
            NODE_13026_length_2854_cov_17.030368, whole genome shotgun
            sequence.
ACCESSION   DAOMYJ010000403 DAOMYJ010000000
VERSION     DAOMYJ010000403.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867850
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prosthecobacter sp. (wastewater metagenome)
  ORGANISM  Prosthecobacter sp.
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Verrucomicrobiaceae; Prosthecobacter.
REFERENCE   1  (bases 1 to 2854)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2854)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.30
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:34:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,742
            CDSs (total)                      :: 4,684
            Genes (coding)                    :: 4,676
            CDSs (with protein)               :: 4,676
            Genes (RNA)                       :: 58
            tRNAs                             :: 55
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2854
                     /organism="Prosthecobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13026_length_2854_cov_17.030368"
                     /isolate="AMR_MDS_5629"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1965333"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..450)
                     /locus_tag="PLP58_22360"
     CDS             complement(<1..450)
                     /locus_tag="PLP58_22360"
                     /inference="COORDINATES: protein motif:HMM:NF012363.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HRJ11341.1"
                     /translation="MKPTRRHLICAIAALLLATPAGAQQAGKKKAGTDRPEPAEKRVY
                     KTAGESKLEIWIWKPQGWKAEDKRPAMVFYHGGGWRGGAPTSFSRQSAQLAARGMVAM
                     SVQYRLTSQPGVGIANCVEDARSAFRWVRAHAGELGIDPDRIAAGGGS"
     gene            complement(541..2187)
                     /locus_tag="PLP58_22365"
     CDS             complement(541..2187)
                     /locus_tag="PLP58_22365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ11342.1"
                     /translation="MKHARVFCILALGGIAPPPSSAEPAPSWHVRILESLLASDSVLP
                     ARPDAHPPATLFNPEAVIPPQCYTRTEARSNPCYVCHQDPIEGRENRMSDAGLQEAYS
                     FSDEGMTNHWLNLFEDRRERAARISDGEIIHYISQDNYSELPARLREAGFKGFIPDLK
                     NLHLGAEAFDEHGHARDGSHWVAFNYKPFPSTFWPTNGSTDDVMIRLPEPYRGGKDGR
                     HSADIYKANLAILEARMKGMDSVTCPPVDENVIGRDLDQDGSLGTARRIVRPAAWAGA
                     AEGLYFETFLYPEGTEFLHTVRYIAVDKSGGITLSTRMKEVRYMRKVKAYSKAMYARK
                     YQTEGYEKEAGNLPGYVDIGHHGLDNGNGWAIHGFIEDRKGRLRALTYEENFSCMGCH
                     NSVGSTIDKTFSFPRKVDGAAGWGCINLRGMPDAPSKGEAKGEFLTYFERVAGGSEFR
                     NNEEMEARWFRPDGTPDPDKLAQARDVYDLVTPSPARAVALNKAYRVIVEDQDYIYGK
                     DTLTAPPANVYDRIDNKTAPTLPADKIYRYNILLDWPAAP"
     gene            complement(2275..>2854)
                     /locus_tag="PLP58_22370"
     CDS             complement(2275..>2854)
                     /locus_tag="PLP58_22370"
                     /inference="COORDINATES: protein motif:HMM:NF017591.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF839 domain-containing protein"
                     /protein_id="HRJ11343.1"
                     /translation="PYGRPEDAAVSRLANGNEVLYVAVTSENAVISIEILTPGTTKVN
                     GTAFVRTFISRATPKNAGFDGTTGQIDSPDNLALDAAGNIYVIEDSPNSGSIGGDIWF
                     ARDTDNDGVAESADHFMTLGIPTSEATGMIFNPTRPTEFWVAVQHPASTDLNAVPNGF
                     GDAVWVFDVSNVSDTGFVKALKRGAFRFISNQ"
BASE COUNT          426 a          796 c         1076 g          556 t
ORIGIN      
        1 ggagccgccg cctgcggcga tgcggtccgg gtcaatgccc agctccccgg cgtgtgcgcg
       61 cacccagcgg aaggcgctgc gcgcgtcctc gacgcagttg gcgatgccca caccgggctg
      121 ggaggtgagg cggtactgca cgctcatcgc caccatgccg cgcgcggcga gctgcgcgct
      181 ctggcgggag aaggaggtgg gcgctccgcc gcgccagccg ccgccgtggt aaaagaccat
      241 ggccgggcgc ttgtcctccg ccttccagcc ctgcggcttc cagatccaaa tctccagctt
      301 gctctcaccc gccgttttat agacccgctt ctcggcgggt tcgggccggt cggtgccggc
      361 tttctttttc ccagcctgct gggcgccggc gggggtggcg agcaggagcg cggcgatggc
      421 gcaaatcagg tggcggcggg tcggtttcat ggcagccact gacacgatgg caggcggggg
      481 attgttgcgc gggattgccg ctgcttttgg gaaggatccc gtgttttctg aacccgctgg
      541 tcagggcgcg gcgggccagt cgaggaggat gttgtaacgg taaattttgt ccgcgggcag
      601 ggtgggggcg gttttgttgt ctatcctgtc atagacgttg gcgggcgggg cggtcagggt
      661 gtccttgccg taaatgtagt cctggtcctc gacgatgacg cggtaggcct tgttcagcgc
      721 cacggcccgc gcgggcgagg gggtgacgag gtcgtaaaca tcacgcgcct gggcgagctt
      781 gtccgggtcg ggtgtgccgt ccgggcggaa ccagcgggct tccatttcct cgttgttgcg
      841 aaattcgctg ccgcctgcga ctcgctcgaa ataggtcagg aactcgcctt tggcctcgcc
      901 tttgctgggc gcgtcgggca tgccgcgcag attgatgcat ccccagccgg cggcgccgtc
      961 cactttgcgc ggaaagctga aagtcttgtc aatagtggag cccacggagt tgtggcagcc
     1021 catgcaggag aagttctcct cgtaagtgag ggcgcgcagc cggcctttgc ggtcctcgat
     1081 gaagccatgg atggcccagc cgttgccgtt gtccagcccg tgatgaccga tgtccacata
     1141 acccggcagg ttgcccgcct ccttttcgta accctcggtc tggtatttgc gggcatacat
     1201 ggccttggag taggctttga ctttgcgcat gtagcgcacc tccttcatgc gggtggaaag
     1261 agtgatgccg ccgcttttgt ccacggcgat gtaacgcacg gtgtgcagga actcggtgcc
     1321 ctcggggtag aggaaggttt cgaaatacag cccctcagcc gcgcccgccc atgcggcggg
     1381 ccgcacgatg cgccgggcgg tgccgaggct gccgtcctgg tcaagatcac gcccgatgac
     1441 gttctcgtcc accggcgggc aggtgacgct gtccatgcct ttcatgcggg cttcgaggat
     1501 ggcgaggttg gccttgtaaa tgtcggcgga gtgcctgcca tccttgccgc cgcggtaggg
     1561 ctccggcagg cggatcatga catcgtcggt ggagccgttg gtgggccaga aggtgctggg
     1621 gaagggtttg taattaaagg ccacccagtg gctgccgtcc ctggcgtggc cgtgctcgtc
     1681 gaaggcttcc gcgccgagat gcaggttttt caggtcgggg atgaagcctt tgaatccggc
     1741 ttcgcgcagg cgcgcgggca gctcgctgta gttgtcctgg gagatgtaat gaatgatttc
     1801 cccgtcgctg attctggcgg cgcgctcgcg gcggtcttcg aaaaggttga gccagtggtt
     1861 ggtcatgccc tcgtcactga agctgtaggc ttcctggagg ccggcgtcgc tcatccggtt
     1921 ctcgcggccc tcgatgggat cctggtggca gacgtagcag ggattggagc gggcctcggt
     1981 gcgggtgtaa cactgcgggg ggatgacggc ctcgggattg aaaagagtcg cgggaggatg
     2041 cgcgtcgggc cgcgcgggca gcacggagtc actggcgagg aggctttcca aaatccgcac
     2101 gtgccaggac ggggccggtt cagcgctgga aggcgggggg gcgatgccac cgagggcgag
     2161 gatgcaaaag acgcgggcgt gtttcatggg gccggaggcg gcgggcgtga ggaaagctgg
     2221 gaagggcggc cccggcgggc cgcccttcgt gggtggattg ctggacggcg gggcttactg
     2281 gttcgagatg aagcggaagg cgccgcgctt gagggctttc acgaagccgg tgtcggacac
     2341 gttggagaca tcgaagaccc agacggcgtc accgaagccg ttggggacgg cgttgaggtc
     2401 ggtgctggcg ggatgctgca cggcgaccca gaattcggtg gggcgggtgg ggttgaaaat
     2461 catgccggtg gcttcggagg tggggatgcc gagggtcatg aagtggtcgg cggattcggc
     2521 cacgccgtcg ttgtcggtgt cgcgggcgaa ccagatgtca ccgccgatgc tgccgctgtt
     2581 cggggagtct tcgatgacat aaatgttgcc ggcggcgtcg agggcgaggt tgtccgggga
     2641 gtcaatctgg ccggtggtgc cgtcaaagcc ggcattcttc ggggtggcgc gggagatgaa
     2701 ggtgcggacg aaagcggtgc cgttgacctt ggtcgtgccg ggggtgagga tctcgatgga
     2761 gatgacggcg ttctccgagg ttacggccac atagagcacc tcgttgccat tggccaggcg
     2821 cgagacggcg gcgtcctccg ggcggccgta aggc
//
LOCUS       DAOMYJ010000404         2849 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Prosthecobacter sp. isolate AMR_MDS_5629
            NODE_13068_length_2849_cov_15.758769, whole genome shotgun
            sequence.
ACCESSION   DAOMYJ010000404 DAOMYJ010000000
VERSION     DAOMYJ010000404.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867850
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prosthecobacter sp. (wastewater metagenome)
  ORGANISM  Prosthecobacter sp.
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Verrucomicrobiaceae; Prosthecobacter.
REFERENCE   1  (bases 1 to 2849)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2849)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.30
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:34:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,742
            CDSs (total)                      :: 4,684
            Genes (coding)                    :: 4,676
            CDSs (with protein)               :: 4,676
            Genes (RNA)                       :: 58
            tRNAs                             :: 55
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2849
                     /organism="Prosthecobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13068_length_2849_cov_15.758769"
                     /isolate="AMR_MDS_5629"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1965333"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2001
                     /locus_tag="PLP58_22375"
     CDS             <1..2001
                     /locus_tag="PLP58_22375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ11344.1"
                     /translation="PSFMPQAGPFQAARPPQQDLAAPVALKPPGDMGGLFAPAGEKSL
                     PAVPPGPSPAPVAPAGDSPFTALFGPKVTPTGQPAPDPSRPGPALPSSNGGGQVRLSV
                     AGLLRGYSVAELGFDPLIVPAWIMTTQPAHDVRQWLELPSPQTNLGVLVDNITDVGFR
                     NVLNNAHREFQVLLAREELMRALQGESPPTLPSLASLKPAAPIFPAQPPSGNGGASPF
                     QVSPSQPPSGNGGASPFQVSPAQPPSGNGGTSPFQVSPAQPPSGNGGASSFQVSPAQP
                     PSGNGGASPFQVSPAQPPSGNGGASPFQVSPAPAPAPQPAPREIKAPVFQVMPPAGAP
                     AAQEPAAAPPSPVFGSPPAQTPFSASPPASPPAQPSPFIVQPATPPGAPAFAQQAPAP
                     GFAAAPAAQNHAPGLITPDSARQPVAHVPVPPQVFPVSPDFTPERAGFGSAELMAAPS
                     APESRPPVPVPQQQQFTPPVERPPQQIAPVFPAIEPAPPPAAQAFIPPVMPAPPASSA
                     PAFTPAASPPRPAHQAAQPALGIQAADSRPDQIMLRALLGTDEELTAQRVVEMTCALP
                     GIAACVCLRGEQALSHIGAHKPQAREFQKQATGLSHHLRSLAPLIGIEGAETFTLTSG
                     DRLMTFCFPENIIFGILHDASPSLGLRDKITLIARELSRMLP"
     gene            2017..2643
                     /locus_tag="PLP58_22380"
     CDS             2017..2643
                     /locus_tag="PLP58_22380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009965103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase domain-containing protein"
                     /protein_id="HRJ11345.1"
                     /translation="MPVLNTEQKTVSFKIVYCGTHLSGKTANLQQIHAKLDPQGRSDL
                     VSLSTARDRTLFFDFLSIESAALPGYKTAFQLYTVPGQVTYNATLQLVLRQADGVVFV
                     ADSQLDRQRDNIQALQALDANLRLNGHSLDRIPLVLQYNKRDLPNTAPVEYLEFLLNN
                     RPRPWLSFEAEARAGKNVLATLNAISQAVLSSFQARQAGRLQAEAVAA"
BASE COUNT          433 a         1168 c          806 g          442 t
ORIGIN      
        1 ccgtcgttca tgccgcaggc cggccccttc caggcggcgc ggccgccgca acaggacttg
       61 gctgccccag tcgcactcaa gcctcctgga gacatgggcg gcctgtttgc tccagccggg
      121 gaaaaaagcc tgcccgccgt gccgcccggg ccatcgcccg cgcctgtcgc acctgcggga
      181 gactcgcctt tcaccgcgct ttttggcccg aaggtcacgc ccaccggcca gcccgcgcca
      241 gatccgtccc gccccggccc ggcgctgccc tcctccaatg gcggcggcca ggtgcggttg
      301 agcgtggccg ggctgctgcg cggctactcg gtggccgagc tcggctttga tccgttgatc
      361 gtgcccgcct ggatcatgac cacccagccc gcgcatgacg tgcggcaatg gctggagttg
      421 ccgagcccgc agacaaacct cggcgtgctg gtggacaaca tcaccgacgt gggtttccgc
      481 aatgtgctga acaacgccca ccgggaattc caggtgctgc tggccaggga ggaactcatg
      541 cgcgccttgc agggcgagtc gcctcccact ctgccatcgc tcgccagcct gaaacctgct
      601 gcaccaatct tccccgctca gccaccctcc ggcaacggtg gtgccagccc cttccaggtc
      661 agcccctcgc agccaccctc cggcaatggt ggtgccagcc cttttcaggt cagccccgcg
      721 cagccaccct ccggcaacgg cggcaccagc cccttccagg tcagccccgc tcagccaccc
      781 tccggcaacg gcggtgccag ctccttccag gtcagccccg cgcagccacc ctccggcaac
      841 ggtggtgcca gccctttcca ggtcagcccc gcgcagccgc cctccggcaa tggtggtgcc
      901 agcccttttc aagtcagccc agcgccagca cccgctccgc agccggcacc gcgcgagatc
      961 aaagcgccgg tttttcaggt catgccgcct gccggcgcgc ctgcggcgca ggaacccgcc
     1021 gcggcgccgc cgtctccggt gttcgggtcg cctcccgcgc agactccatt ttcagcgtct
     1081 ccgcccgcat ctccacccgc ccagccgagt cccttcatcg tgcagcctgc cacgccgcca
     1141 ggcgcgcctg cgttcgctca acaggcgccg gcgcccggtt ttgcggccgc tcctgccgcg
     1201 caaaatcatg cgcctggctt gatcacaccc gactccgcgc ggcagcccgt cgcccatgtt
     1261 ccggtgccgc cgcaggtctt tcctgtcagt ccggacttca ccccggagcg cgccgggttt
     1321 ggcagcgcgg aactgatggc cgccccatcc gctcccgaga gcaggccgcc cgtgcccgtg
     1381 ccgcagcagc agcagttcac gccgccagtg gaaagaccgc cgcagcagat cgcgcccgtc
     1441 tttcccgcca tcgagcctgc tccgccgcct gccgcccagg cattcatccc gccggtgatg
     1501 ccagcgccac cggcatcgtc agcgccggcg ttcacacccg ccgccagtcc gccgcgccca
     1561 gctcaccagg ccgcccagcc cgcgctcggc attcaggcgg ctgacagcag gccggaccag
     1621 atcatgctgc gcgccctgct cggcacggat gaggagctga ctgcgcagcg ggtggtggaa
     1681 atgacctgcg ccctgcccgg catcgccgcc tgcgtctgcc tgcgcgggga gcaggcgctt
     1741 tcccacatcg gcgcgcacaa gccgcaggcg cgcgagttcc agaagcaggc caccggcctt
     1801 tcccatcatc tgcgcagcct ggccccgctc atcggcatcg agggcgcgga gaccttcacc
     1861 cttacttccg gcgaccgcct gatgaccttt tgcttcccgg aaaacatcat cttcggcatc
     1921 ctgcacgacg cctctccctc cctcggattg cgggacaaga tcaccttgat cgcgcgcgag
     1981 ctttcacgga tgctgccctg aacccgccgc gccgccatgc ccgtcctcaa caccgagcaa
     2041 aagactgtct ccttcaagat tgtctattgc ggcacgcacc tcagcggcaa gacggcgaac
     2101 ctccagcaga tccacgccaa gctcgacccc cagggccgca gcgaccttgt cagcctctcc
     2161 accgctcgcg accgcaccct gttcttcgac ttcctcagca tcgaaagcgc cgccctgccc
     2221 ggctacaaga ccgccttcca gctctacacc gtgcccggcc aggtcactta caacgccacc
     2281 ctccagctcg tgctgcgcca ggccgacggc gtcgtcttcg tggccgacag ccagctcgac
     2341 cgccagcgcg acaacattca ggccctccag gctctcgacg ccaatctccg cctcaacggc
     2401 cactccctgg accgcatccc gctcgtgctg caatacaaca agcgcgacct gcccaacacc
     2461 gccccggtgg aatacctcga attcctcctc aacaaccgcc cccggccctg gctcagcttc
     2521 gaggcggagg cgcgcgcggg gaaaaacgtg ctggccaccc tcaacgccat ctcccaggcc
     2581 gtgctctcca gctttcaagc gcgccaggcc gggcgcttgc aggcagaggc ggtggccgca
     2641 tgagcgccgc gtttgttttc aaatggcgtc accacgctga cgcactatca tgaccacgcc
     2701 cggcctgtgc cgggaacacc catccccagc cccgaccgag tgaccgccgc cctcatccag
     2761 cctgatggac atttccagct tctctgcgga gaggccgccg ccgacaccgc ctgcgtcgcc
     2821 gcgctcgccc acgccgtgcg cgcggcggg
//
LOCUS       DAOMYJ010000405         2831 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Prosthecobacter sp. isolate AMR_MDS_5629
            NODE_13180_length_2831_cov_18.559078, whole genome shotgun
            sequence.
ACCESSION   DAOMYJ010000405 DAOMYJ010000000
VERSION     DAOMYJ010000405.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867850
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prosthecobacter sp. (wastewater metagenome)
  ORGANISM  Prosthecobacter sp.
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Verrucomicrobiaceae; Prosthecobacter.
REFERENCE   1  (bases 1 to 2831)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2831)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.30
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:34:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,742
            CDSs (total)                      :: 4,684
            Genes (coding)                    :: 4,676
            CDSs (with protein)               :: 4,676
            Genes (RNA)                       :: 58
            tRNAs                             :: 55
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2831
                     /organism="Prosthecobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13180_length_2831_cov_18.559078"
                     /isolate="AMR_MDS_5629"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1965333"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            102..608
                     /gene="gspG"
                     /locus_tag="PLP58_22385"
     CDS             102..608
                     /gene="gspG"
                     /locus_tag="PLP58_22385"
                     /inference="COORDINATES: protein motif:HMM:TIGR01710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system major pseudopilin GspG"
                     /protein_id="HRJ11346.1"
                     /translation="MNTRTSPTKLEARNSKSPFRIPQSAFRNCHSAFTLIEIVIVLTI
                     ISILAAGSIYMLKGNVDVAKETRVDGDLQNIMTQLQLYEARNMRPPTTEQGLKALVEK
                     PTTEPLPEKWTALLEEVPKDPWGMEYKYRYPAQKSKKAYDVYSVGKDGVEGTEDDIGN
                     WKATATAP"
     gene            605..1327
                     /locus_tag="PLP58_22390"
     CDS             605..1327
                     /locus_tag="PLP58_22390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ11347.1"
                     /translation="MRTLPEHRSSNFEFRISNFDLQEFSIHPGSTARAFTLVEIVISL
                     TIIIVIAAASIPTIRGIRDEQLAREPIIELTRMAKEARLRAMKEKRPYQVAFYSGGFM
                     ASRYFNPYLQLSDLDLLAQELESGVSRVNPNEDPTLVDLDSGATTTPKTQLPLAPPGP
                     KLDNDWQERYELPPGSQYTLKFWHDLEETPVEGEIVKLWVFQPTGICQPLQLRLENSS
                     ASFSIEYGALTADIIREVIDLR"
     gene            1465..1893
                     /locus_tag="PLP58_22395"
     CDS             1465..1893
                     /locus_tag="PLP58_22395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ11348.1"
                     /translation="MRPSQRSALPCRLKASLKRGYILLELVIALSMFAIGVLGLARAL
                     NTSMEVANILNKDQRVRIGMRSFLEEVRRKPLAEMSTTFTDAASGVTYTSTAERIVLN
                     TTRGGTLSDMYNLKITAAYSVGDEQREETVSVYVYKPAQR"
     gene            1967..>2831
                     /locus_tag="PLP58_22400"
     CDS             1967..>2831
                     /locus_tag="PLP58_22400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HRJ11349.1"
                     /translation="MVPKTHLQSTPVSTSQWFKTASAFTLMEVVIALTILGMITGTLF
                     AIIQGSVRAAAQIEQIQRENDGINRFLELCRKTFTTLPSTATLTLSAIGGNLDQGQEL
                     TISGAPNCFGFGARPISYSDTILGLRPDPNGDVDSNEAPLNYLCLSREDLISQADNRD
                     MAVRQEITGLAAPDDQGRYWMPLLPDVVALKWRFYKEADDTWLEEWSDSNWPDLIEVQ
                     LLMRDRQTALRMVYSVPVIQLVAGTGTSSTPSSSTGGTGAAAGGGGGPGGGRGGPGGG
                     GDGGGRGDGGDP"
BASE COUNT          620 a          966 c          751 g          494 t
ORIGIN      
        1 aaagcgttta tcaaacccgg agagcactga agaagttcgt cgacgaacag atgcacccat
       61 cctgtagcat ccgaaaaaat cccgaatctc cctccaccgc catgaacacc cgcacctccc
      121 caacgaaact cgaagctcga aattcgaagt cgccattccg cattccgcaa tccgcattcc
      181 gcaattgcca ctccgccttc acactcatcg agatcgtcat cgtactgacg atcatctcca
      241 tcctcgccgc cggctccatc tacatgctca aaggcaacgt ggatgtcgcc aaggaaaccc
      301 gcgtggatgg cgacctgcaa aacatcatga cccagcttca actttacgaa gcccgcaaca
      361 tgcgcccgcc caccaccgag cagggcctca aggcgctcgt cgaaaaaccc accaccgagc
      421 cgctgccgga aaaatggacc gccctgcttg aggaggtgcc taaagacccc tggggcatgg
      481 aatacaaata ccgttacccc gcccaaaaat ccaaaaaggc ctacgatgtc tattccgtcg
      541 gcaaggacgg cgtcgaaggc accgaggatg acatcgggaa ctggaaagca actgcgacag
      601 cgccatgaga actcttcctg agcaccgaag ctcgaatttc gaatttcgaa tttcgaattt
      661 cgatcttcag gagttttcca ttcatccggg aagcaccgcc cgcgctttca ccctcgtcga
      721 gatcgtcatc tccctgacga tcatcatcgt catcgccgcc gcctccattc ctaccatccg
      781 gggcattcgt gatgagcagt tggcccgcga gcccatcatc gagctgacgc gaatggccaa
      841 ggaggcgcgg ctgcgggcga tgaaggaaaa gcggccctac caggtggcct tttactccgg
      901 cggcttcatg gcctcacgct acttcaatcc ctacctccag ctttccgacc tcgacctcct
      961 ggcgcaggag cttgagtcag gcgtcagccg cgtgaacccg aacgaggatc cgaccctcgt
     1021 ggacctcgac tccggcgcca ccaccacgcc gaagacccag cttcccctgg caccgcccgg
     1081 cccgaaactc gacaacgact ggcaggagcg ctacgagttg ccgcccggct cgcaatacac
     1141 cctcaaattc tggcacgact tggaggagac acctgtcgag ggcgagatcg tcaagctctg
     1201 ggtcttccag cccaccggca tctgccagcc cttgcaactg cgcctggaaa actccagcgc
     1261 cagtttcagc atcgaatacg gcgcgctcac cgccgacatc atccgggagg tgattgacct
     1321 gcgatgaaga cggcccacgt tggagttcaa gctttagctt gcccgggagt tgaccacgca
     1381 acggagtcca ctcccgtgtc gcctaaaggc ttcactccag cggtcttcac tccaattgtt
     1441 ggagttcaag ctttagcttg ttctgtgcga ccctcgcagc ggagcgcact cccgtgtcgc
     1501 ctcaaggctt cactcaaacg cggctacatc ctgctcgaac tcgtcatcgc cctgagcatg
     1561 ttcgccatcg gcgtgctggg cttggcgcgc gcgctgaaca cctccatgga agtggcgaac
     1621 atcctcaaca aagaccagcg cgtgcgcatc ggcatgagat cctttctgga ggaggtgcgg
     1681 cgcaaaccgc tggccgagat gagcaccacc ttcaccgatg ccgccagcgg cgtcacctac
     1741 acaagcaccg ccgagcgcat cgtgctcaac accacccgcg gcggcaccct gagcgacatg
     1801 tacaacctca aaatcaccgc cgcctacagc gtgggcgacg aacaacggga ggaaaccgtc
     1861 tccgtctatg tgtacaaacc cgcgcaacgg tgagagtcac gaacttcgaa cttcgaaact
     1921 cgaagttcga agttcccatt cattagtgtc catcagtgtt ccattagtgg ttccaaaaac
     1981 tcatctccaa tcaaccccag tcagcaccag tcagtggttc aaaaccgcca gcgccttcac
     2041 cctgatggag gtcgtcatcg ccctgacgat cctcggaatg atcaccggca cgcttttcgc
     2101 catcatccag ggcagcgtgc gcgcggcggc gcagatcgag caaatccagc gcgagaacga
     2161 cggcatcaac cgcttcctgg aactctgccg caagaccttc accaccctgc ccagcaccgc
     2221 cacgctcacc ctcagcgcca tcggtggcaa cctcgaccaa ggacaggaac tcaccatcag
     2281 cggcgcgccg aactgcttcg gcttcggcgc gcgccccatc tcttacagtg acaccatcct
     2341 cggtctgcga ccagacccca acggagacgt ggacagcaac gaggcgccgc tgaactacct
     2401 ctgcctgtcc cgcgaggatt tgatctcaca ggccgacaac cgcgacatgg ccgtgcgcca
     2461 ggagatcacc ggcctggccg cgccggatga ccagggccgc tactggatgc cgcttttgcc
     2521 ggatgtcgtg gctttgaaat ggcgcttcta taaggaagcg gacgacacct ggctggagga
     2581 atggtcggac tcgaactggc cggatctcat cgaagtccag ctcctcatgc gcgaccgcca
     2641 gaccgcgctg cgcatggtct atagcgtgcc ggtcatccag ctcgtcgccg gcacaggcac
     2701 ttcgtccacc ccatcctcca gcacgggtgg cacaggcgcg gcagcagggg gaggtggcgg
     2761 tccaggcggc ggtcgtggag ggccgggcgg aggtggtgat ggtggcggcc gcggagacgg
     2821 gggcgatccg g
//
LOCUS       DAOMYJ010000406         2828 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Prosthecobacter sp. isolate AMR_MDS_5629
            NODE_13203_length_2828_cov_28.912009, whole genome shotgun
            sequence.
ACCESSION   DAOMYJ010000406 DAOMYJ010000000
VERSION     DAOMYJ010000406.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867850
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prosthecobacter sp. (wastewater metagenome)
  ORGANISM  Prosthecobacter sp.
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Verrucomicrobiaceae; Prosthecobacter.
REFERENCE   1  (bases 1 to 2828)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2828)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.30
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:34:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,742
            CDSs (total)                      :: 4,684
            Genes (coding)                    :: 4,676
            CDSs (with protein)               :: 4,676
            Genes (RNA)                       :: 58
            tRNAs                             :: 55
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2828
                     /organism="Prosthecobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13203_length_2828_cov_28.912009"
                     /isolate="AMR_MDS_5629"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1965333"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..549)
                     /gene="mutM"
                     /locus_tag="PLP58_22405"
     CDS             complement(<1..549)
                     /gene="mutM"
                     /locus_tag="PLP58_22405"
                     /EC_number="3.2.2.23"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: protein motif:HMM:NF002211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008534 - oxidized purine nucleobase
                     lesion DNA N-glycosylase activity [Evidence IEA];
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA-formamidopyrimidine
                     glycosylase/DNA-(apurinic or apyrimidinic site) lyase"
                     /protein_id="HRJ11350.1"
                     /translation="MPELPEVETTLRGVSPWLVGRVVREVIVREKRLRWPVPDEIHEL
                     EGRRIVSGRRRGKYLLFESPAGTLLLHLGMSGSLRVSEPEAPWKKHDHLAFTTDHDRQ
                     IRLHDPRRFGAALWLTGDAEAHPLLADLGPEPLGADFTAEHLRARCQERAAPVKAVIM
                     DSHVVTGVGNIYACEALFMAGIR"
     gene            complement(620..1576)
                     /locus_tag="PLP58_22410"
     CDS             complement(620..1576)
                     /locus_tag="PLP58_22410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009962019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxy-5-phosphonooxypentane-2,4-dione
                     thiolase"
                     /protein_id="HRJ11351.1"
                     /translation="MADLSTSAADKKEKEFFTHVPQEAPGFFLKGSHQYDWGLKNRLS
                     RVFNPKDGRTVMLAFDHGYFQGPTTGLERVDQTILPLEPYADCLMLTRGIQRSIIPAS
                     TQKAIALRASGGTSMVSPFEEWEGEIGDKKFKLGRPGFEPLSNESTALSIEEAIRLNA
                     SVLAVQVFIGSAYERQSLKNLTDLVDAASRYGIGVMGVVAVGRAMARNAQYFRLATRI
                     MAELGANVVKCYYTDEGFDTITSCCPVPIVIAGGKKLPELDALTMCHNAIQQGASGVD
                     MGRNIFQSDAPVAMMQAVRGVVHEGLTPAQGYQMYQDLKTGG"
     gene            complement(1755..2393)
                     /locus_tag="PLP58_22415"
     CDS             complement(1755..2393)
                     /locus_tag="PLP58_22415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009959145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trimeric intracellular cation channel family
                     protein"
                     /protein_id="HRJ11352.1"
                     /translation="MPPWIEYSAVAVCAISGVLAAEGKRMDLFGALVLALVTAVGGGT
                     LRDLCLGAEPVFWIRSPAHVTTALGAALATFILARFVVFPARALALADAFGLALFGIV
                     GTEKALLFDSPAIVAILLGVVTGVAGGILRDVLRQEVPEVFRTEIELYATAVFAGALV
                     YVLLRQWLPPSEAHRYIGMGVILLMRLAAMRWRLRLPAYRSRSHVPPGEGGK"
     gene            complement(2577..>2828)
                     /locus_tag="PLP58_22420"
     CDS             complement(2577..>2828)
                     /locus_tag="PLP58_22420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ11353.1"
                     /translation="RQTAQLPLALQGDTAASARRQADSLLSDLSALSLDALARAPGTV
                     TEAVPAFCASFTAGLEKLSDLITEHYFSHAAARNGRREG"
BASE COUNT          504 a          884 c          971 g          469 t
ORIGIN      
        1 ccggatgccg gccatgaaaa gcgcctcgca ggcgtagatg ttgcccacgc ccgtgaccac
       61 atgcgagtcc atgatgacgg ccttgacggg cgcggcgcgc tcctggcagc gggcgcgcag
      121 atgctccgcc gtgaagtcag cccccaaagg ttcggggccg agatcggcga gcaggggatg
      181 cgcttcggca tctcccgtga gccagagcgc cgcgccgaac cggcgcggat catgcaggcg
      241 gatttgcctg tcatgatcgg tggtgaaagc cagatgatcg tgctttttcc acggtgcttc
      301 gggttcactc acgcgcaggc tgccggacat gccgagatgc agcagcagcg tgcccgccgg
      361 gctttcaaaa agcaggtatt tcccgcgcct ccgcccggag acaatgcgcc gcccctccag
      421 ttcatggatc tcatccggca ccggccagcg caggcgtttc tcccgcacaa tgacctcacg
      481 caccacgcgc cccaccagcc acggggagac gccccgcagc gtggtttcga cttcgggcag
      541 ttcaggcatg gggaatgtgg aaactcgaaa ttcgaagacc gaaactcaaa gttgaagatc
      601 gagtctggat ttcggatttt cagcccccgg tcttgaggtc ctggtacatc tggtagccct
      661 gcgcgggcgt cagcccctca tgcaccacgc cgcgcaccgc ctgcatcatc gccaccggcg
      721 cgtcgctctg gaagatgttc cggcccatgt ccaccccgct ggctccctgc tggatggcgt
      781 tgtggcacat cgtcaaggcg tcgagctccg gcagcttctt gccccccgcg atcacgatgg
      841 gcaccgggca gcaggaggtg atggtgtcaa agccctcgtc ggtgtagtag cacttcacca
      901 cattggcccc caactccgcc atgatgcgcg tggccaggcg gaaatactgc gcgttgcgcg
      961 ccatcgcccg gcccacggcc accacgccca tcaccccgat gccgtagcgg ctcgccgcgt
     1021 ccaccaggtc ggtgaggttt ttcaacgact gccgctcata cgcgctgccg atgaagacct
     1081 gcacggccaa aaccgaggcg ttgagccgga tggcctcctc gatgctcagc gcggtgcttt
     1141 cattggacag cggctcgaag ccggggcgcc cgagcttgaa cttcttgtcg ccgatctcgc
     1201 cctcccactc ctcaaagggg ctgaccatgc tcgtgccgcc cgaggcgcgc agggcgatgg
     1261 ccttctgcgt gctcgccggg atgatgctgc gctggatgcc gcgcgtgagc atgaggcagt
     1321 ccgcatacgg ctccaggggc aggatggtct ggtccacgcg ctccaggccc gtggtggggc
     1381 cctggaagta gccgtggtcg aaggccagca tgacggtgcg cccgtccttg ggattgaaga
     1441 ccctggagag gcggttcttc aggccccagt catactggtg ggagcccttg agaaagaagc
     1501 cgggcgcctc ctgggggacg tgggtgaaga attccttttc cttcttgtct gctgcgctgg
     1561 tgctgaggtc ggccatggtg tgcggtgggt tggtgtttga gagggccgat catacgcttc
     1621 gggcacggtt catccatgcc ggactcggcc agatttgtgt caaatcccgc aatcccagcg
     1681 ggtttgccgg ctggtttgcc ccggcgcaca gctttcagaa aatggcaagg ggatggcgcc
     1741 atgaagcacc ttccttattt cccgccttcg cccggcggga catggctgcg ggagcgatag
     1801 gctgggagtc gcagacgcca ccgcatggcg gccaggcgca tgagcaggat gactcccatg
     1861 ccgatgtaac gatgcgcttc ggagggaggc agccattggc gcagcagcac atagaccagc
     1921 gccccggcga agacggcggt ggcataaagt tcgatctcgg tgcgaaagac ctccggcacc
     1981 tcctggcgca gcacatcccg caaaatgccg cccgccacgc cggtgacgac gcccagcagg
     2041 atggcgacga tggcggggga gtcaaaaagc agggctttct ccgtacccac gatgccaaag
     2101 agcgccaggc cgaaggcatc cgccagagcc agggcgcggg cggggaagac cacgaagcgc
     2161 gccaggatga aggtggccag cgcggctccg agcgcggtgg tgacatgggc tggggagcga
     2221 atccagaaaa ccggctcggc gccaaggcac aaatcccgca gcgtgccgcc gcccacggcg
     2281 gtgaccaggg ccagcaccag cgcgccaaag aggtccatgc gcttgccttc ggcagccagc
     2341 acgcctgaga tggcgcagac ggcgacggcg gagtattcga tccagggggg catggcggcg
     2401 gggggatggc gggtgaatga tggatggaag gcgcggaccc ggcaagcccg agcggcttgg
     2461 tgaaaccgac actctcgcct gttttcgaga ggcaaagaca ggagtgcccg cgccacggcg
     2521 aagcgagcct caaatcgtgg cttcacttca tctggattca atcagcgggc gttcattcag
     2581 ccttcccggc gtccgttgcg ggcggcggcg tggctgaagt agtgctcggt gatgaggtcg
     2641 gagagctttt ccagtccggc ggtgaaactg gcgcaaaaag ccgggacggc ttcggtgacg
     2701 gtgccggggg ctcgggcaag ggcgtcgagg ctcagggcgg agaggtcgga aagcagggag
     2761 tcggcctggc ggcgggcgct ggcggcggtg tcaccttgaa gggcgagcgg aagctgggcg
     2821 gtctggcg
//
LOCUS       DAOMYJ010000407         2825 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Prosthecobacter sp. isolate AMR_MDS_5629
            NODE_13217_length_2825_cov_15.470397, whole genome shotgun
            sequence.
ACCESSION   DAOMYJ010000407 DAOMYJ010000000
VERSION     DAOMYJ010000407.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867850
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prosthecobacter sp. (wastewater metagenome)
  ORGANISM  Prosthecobacter sp.
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Verrucomicrobiaceae; Prosthecobacter.
REFERENCE   1  (bases 1 to 2825)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2825)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.30
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:34:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,742
            CDSs (total)                      :: 4,684
            Genes (coding)                    :: 4,676
            CDSs (with protein)               :: 4,676
            Genes (RNA)                       :: 58
            tRNAs                             :: 55
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2825
                     /organism="Prosthecobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13217_length_2825_cov_15.470397"
                     /isolate="AMR_MDS_5629"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1965333"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            208..747
                     /locus_tag="PLP58_22425"
     CDS             208..747
                     /locus_tag="PLP58_22425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ11354.1"
                     /translation="MLFSTVLTWLLIAVAFVIALPSLWLLGQALWPQAMWRRRVAAQR
                     GLWKTFLLGLIPVTGGIVLVSILAKLPQMGALAAVTGGVLMAWGFLGAGGIAALVGER
                     LWGGAAPWRQMMRGGFVVIGCALLPVVGWVVLLPLLAVIGWGINVRAWFVKMPPQPVE
                     VETAPAAPASPPAIPEAAV"
     gene            762..2258
                     /locus_tag="PLP58_22430"
     CDS             762..2258
                     /locus_tag="PLP58_22430"
                     /inference="COORDINATES: protein motif:HMM:NF020390.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1800 domain-containing protein"
                     /protein_id="HRJ11355.1"
                     /translation="MSLDRRALLKTAACGAGGAWLSGCERAATEINRRFFGEGVPARL
                     DVADAPEIDPDFHLLSRAAFGPWPGDLQRLKSMGREAWLEEQLNPSSISDTACDLRAE
                     RFESLYFSAGDAYEFRKPVLREEITRHSLLRAIYSRRQLFEVMVEFWTDHLNIDLEKG
                     DCVYLKPEDDREVARKHALGNFRDLIRASATSPAMLVYLDGVSNKVRKDTDDRPNENY
                     ARELLELHTLGVHGGYTQQDVLEAARCLSGWTFDRKRFLALDAGRSFFKPAWHDNGEK
                     RLLGQVIPAGGGAADLDRLVDIVCAHPGTARFIAEKLCRRFVSLEPPGALVQSVAAEF
                     SRARGDIPSLLRVIFRSQEFMDARGRLLKRPFKFMVSALRALAADTRAEKAILEPLQR
                     MGHGLFQYPTPDGYPDDELPWMGTLMWRWNFALALAGGRQPGVRVGRAPLEKALSRGK
                     TKPALETWFAHFTGRAPTSAERTALEVFPGDLPQMAGLILASPAFQRC"
     gene            complement(2267..2339)
                     /locus_tag="PLP58_22435"
     tRNA            complement(2267..2339)
                     /locus_tag="PLP58_22435"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2304..2306),aa:Ile,seq:cat)
     gene            2429..>2825
                     /locus_tag="PLP58_22440"
     CDS             2429..>2825
                     /locus_tag="PLP58_22440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ11356.1"
                     /translation="MSENSTPPPRLLPVMGFTVLYMTAAIAGSWLQGNREFVFYIAVM
                     IVLISVMMLVHRAARLTGGLLWALSMWGLLHMAGGLVPVPESWPYNGEHAVLYSLWLI
                     PDLLKYDHIVHAYGFGVTTWLCWHALSRSV"
BASE COUNT          456 a          976 c          892 g          501 t
ORIGIN      
        1 tcctggcgca cgagggcgcg cttttccgcg tcctcaacgc gagcgtgcgg cgcgccgatc
       61 tgccagtcgg cggtgtggag aaaagtcatc acgcatggaa agcgagaaaa ttgaaattcg
      121 aaattcgaaa ttcagactgc gaacagcgaa ccgcctcgtt accaccacgg ctacagccgc
      181 gttagcctcc tgtcccaccc accgcccatg ctgttctcca ccgtcctcac ctggctgctc
      241 atcgcagtgg ccttcgtcat tgccctgcca tctctctggc tgcttggcca ggccctctgg
      301 ccgcaggcga tgtggcggcg gcgggtggcc gcgcagcgcg gtctttggaa aacattcctt
      361 ctcggcctca tcccggtgac aggcggcatc gtgctggtct cgatcctggc gaagctgccg
      421 cagatgggcg cgctggcggc cgtcacaggc ggagtcctga tggcatgggg tttcctcggc
      481 gcgggcggca tcgcggcact ggtcggcgag cgactctggg gcggggccgc cccctggcgg
      541 cagatgatgc gcggcggctt cgtcgtcata ggctgcgcct tgctgccggt cgtgggctgg
      601 gtggtgctgc tgccgctgct ggcggtgatc ggctggggga tcaacgtgcg ggcctggttt
      661 gtcaaaatgc cgccgcagcc cgtggaagtg gaaaccgcac ccgccgcgcc tgcctcaccg
      721 ccagccatcc cggaagccgc cgtttgaatt tcaccgccgt catgagcctc gaccgccgcg
      781 ctctgctgaa aaccgccgcc tgcggcgcgg gcggcgcgtg gctttccggc tgcgagcgcg
      841 ccgccacgga gatcaaccgc cggttcttcg gcgaaggcgt gcccgcaaga ctcgatgtgg
      901 cggacgcgcc ggagattgac ccggattttc acctgctctc ccgcgccgct ttcggcccct
      961 ggcccggcga tctccagcgg ctcaagtcaa tggggcgcga ggcatggctg gaggagcaac
     1021 tcaacccctc atccatttcc gacaccgcct gcgatctgcg cgcggagcgt ttcgagtccc
     1081 tctacttcag cgccggggac gcctacgagt tccgcaaacc ggtgctgcgc gaggagatca
     1141 cccgccacag cctgctgcgc gccatttaca gccgaaggca gctcttcgag gtgatggtgg
     1201 agttctggac cgaccatctc aacatagacc tggaaaaggg cgactgcgtc tatctcaagc
     1261 ccgaggacga ccgcgaggtg gcgcgcaaac acgcgctggg gaatttccgc gacctcatcc
     1321 gcgcctccgc cacctccccg gccatgctcg tctatctcga cggcgtctcg aacaaagtcc
     1381 gcaaagacac cgatgaccgg cccaatgaaa actacgcgcg cgagctgctg gagctgcaca
     1441 cactcggcgt gcatggcggc tacacgcagc aggatgtgct ggaggccgcc cgctgcctga
     1501 gcggctggac ctttgaccgc aaacgcttcc tcgcgctgga cgccgggcgc tcttttttca
     1561 aacccgcatg gcatgacaac ggcgaaaagc gcttgctcgg ccaggtcatt cccgcaggcg
     1621 gcggcgcggc ggacctcgac cggctcgtgg acatcgtctg cgcgcatccc ggcaccgcgc
     1681 gcttcatcgc ggaaaaactc tgccgccgct tcgtcagcct ggagccgccc ggcgcgctgg
     1741 tgcaaagcgt ggccgccgag ttcagccgcg cgcgcggtga catcccgtcg ctgctgcgcg
     1801 tcattttccg ctcgcaggag ttcatggacg cgcgcggacg gcttttgaaa aggcccttta
     1861 aattcatggt gtccgccttg cgcgcgctgg ctgcggacac ccgcgcggag aaagccatcc
     1921 tggagccgct gcagcgcatg ggacacggcc ttttccaata ccccacgccc gacggctacc
     1981 ccgacgacga actaccctgg atgggcacgc tcatgtggcg gtggaacttc gcgctggcgc
     2041 tcgccggcgg caggcagcca ggagtccgtg tcgggcgcgc cccgctggaa aaagcgctca
     2101 gccgggggaa aacaaaaccc gcgttggaaa cctggttcgc tcatttcacg ggccgcgccc
     2161 ccacatcagc ggagcgcacg gcgctggaag ttttcccggg ggatcttcca cagatggccg
     2221 gactcatcct cgcctcaccc gcgtttcagc gctgctgaag caggcgtgct cccgaccgga
     2281 ctcgaaccga taaccaagga attatgagtt ccctgctcta accattgagc tacaggagcg
     2341 tctgcgccgc gcgcgggatc atggagccga tgcgccgccg tggcaagtcc ggctcgacag
     2401 gccggatgat ttgtgatgcc atgagcgcat gagcgaaaat tccacacccc cgccgcgcct
     2461 gctccccgtc atgggcttca ccgtcctcta catgaccgcc gccatcgccg gctcctggct
     2521 ccagggcaac cgggagtttg tcttttacat cgccgtcatg atcgtgctga tctccgtgat
     2581 gatgctcgtc caccgcgcgg cgcggctcac cggcggcctg ctgtgggcgc tgagcatgtg
     2641 ggggctgctg cacatggcgg gcgggctggt gccggtgccg gaaagctggc cctacaatgg
     2701 cgagcacgcg gtgctttaca gcctctggct catccctgac ctgctcaaat acgaccacat
     2761 cgtccacgcc tacggcttcg gcgtcaccac ctggctgtgc tggcacgcgc tgagccgctc
     2821 cgtcc
//
LOCUS       DAOMYJ010000408         2814 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Prosthecobacter sp. isolate AMR_MDS_5629
            NODE_13281_length_2814_cov_19.633200, whole genome shotgun
            sequence.
ACCESSION   DAOMYJ010000408 DAOMYJ010000000
VERSION     DAOMYJ010000408.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867850
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prosthecobacter sp. (wastewater metagenome)
  ORGANISM  Prosthecobacter sp.
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Verrucomicrobiaceae; Prosthecobacter.
REFERENCE   1  (bases 1 to 2814)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2814)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.30
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:34:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,742
            CDSs (total)                      :: 4,684
            Genes (coding)                    :: 4,676
            CDSs (with protein)               :: 4,676
            Genes (RNA)                       :: 58
            tRNAs                             :: 55
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2814
                     /organism="Prosthecobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13281_length_2814_cov_19.633200"
                     /isolate="AMR_MDS_5629"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1965333"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(79..1887)
                     /locus_tag="PLP58_22445"
     CDS             complement(79..1887)
                     /locus_tag="PLP58_22445"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF039989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acyltransferase"
                     /protein_id="HRJ11357.1"
                     /translation="MILDPGEHLPAAWQRGLYRAFSPFFEGPLGLRGLEEIYQEAARL
                     QDARSEGRTCEAWFESVLEAMDASGDVDEAPEFEFPKSGPLVIVANHPFGILDPVVLA
                     HYAARQRPDVKVMTNSLLGRFERLRPHIIPVNPFGGAEAARENLAGMKEAMRHLRGGG
                     ALVIFPAGEVSRWRPGRGIEDAAWSSHAAALVRRTGAAVLPVYFPGRNSALFHAAGLV
                     HPRLRTGLLLREFCNKRGTRVEMRAGAVIPFAKLRKFEDDEALTKYLRVHTFILAGRR
                     QANDGRSADTQADTPPADRFHHERHQQRIQAEVRHLRGHGACLASQGGFGVYAAHSHE
                     IPETLQEIGRLREVAFRQVGEGTGQEVDLDRFDRYYEHLFLWDEEKGRVAGAYRLGRA
                     DMILREYGPRGLYTSTLFKFEKPFLASLGNAVEMGRSFVAREYQRNLSSLPLLWRGIA
                     QWMVRNGGYTKLFGPVSISKDYDSLSRKMMVEFLQENCLHEDLASYVKPRNPFRYLRG
                     RRMMREFISASLRDVDDCSALISSVETDGKGIPVLLKHYLRLSGTILSFNVDREFSSV
                     IDGLILVDLTETEPRLLAKYMGEENCRAWLARHAPR"
     gene            2047..>2814
                     /gene="prmC"
                     /locus_tag="PLP58_22450"
     CDS             2047..>2814
                     /gene="prmC"
                     /locus_tag="PLP58_22450"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009960850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HRJ11358.1"
                     /translation="MSKPLLETLHAGAGYLEKHGVEEARLNMEHLLAHVLGCRRLDLY
                     LRHAEVPREDDLARLRELAKRRASGEPLQHLLGTVDFLGHELVCDERALIPRPETEYL
                     CDLLIKASRAAPPVRVLDMAAGSGCIGLTLAAAWPGSQVTLADISEDALDLARLNAGR
                     LGLDAVRIVRSDLFEKISGGFDLIVSNLPYIPSAEIATLSREVRRDPALALDGGPDGL
                     DIVRRFLAAAPAHLNAEGGIALDTASSRARLHGDKRIQ"
BASE COUNT          444 a          990 c          903 g          477 t
ORIGIN      
        1 cctggcgaac tgggacgcca tccggctgcc ggcgctggtg cgccccggcg cgcgtgtcat
       61 cgtgcgttga gcgccgcctc agcgcggcgc gtgcctggcc agccaggcgc ggcagttttc
      121 ctctcccatg tatttggcca gcaggcgcgg ctcggtctcg gtgaggtcca cgaggatgag
      181 gccgtcaatc acggaggaga actccctgtc cacgttgaag ctgaggatgg tgccgctgag
      241 gcggaggtag tgcttgagca ggacggggat gcccttgccg tcggtctcga cgctggagat
      301 gagcgcggag cagtcgtcca cgtcgcgcag gctggcgctg atgaactcgc gcatcatcct
      361 cctgccgcgc aggtagcgga aggggttgcg cggcttcaca tagctggcca ggtcctcatg
      421 caggcagttc tcctggagaa actccaccat catcttccgg ctcaggctgt cgtagtcctt
      481 gctgatgctg accggcccga agagcttggt gtaacccccg ttgcgcacca tccattgggc
      541 gatgccgcgc cagagcaggg gcagggagga aagattgcgc tggtattccc tggcgacgaa
      601 gctgcgcccc atctccacgg cgttgccgag gctggcgagg aagggctttt cgaatttgaa
      661 gagcgtgctg gtgtaaagac ccctgggtcc gtattcgcgc aggatcatgt cggcgcggcc
      721 caggcggtag gctccggcga cgcggccttt ttcctcgtcc cagagaaaca ggtgctcgta
      781 atagcggtcg aacctgtcca ggtccacctc ctgccctgtg ccctcgccca cctgccggaa
      841 ggcgacctcc cgcagccgcc cgatctcctg gagagtctcg gggatctcgt gcgagtgcgc
      901 ggcatagacg ccgaagccgc cctggctcgc gaggcaggcc ccgtgcccgc gcagatgacg
      961 cacctccgcc tggatgcgct gctggtggcg ctcgtggtgg aagcggtcgg cgggcggggt
     1021 gtccgcctgt gtgtccgcgg agcgcccgtc attggcctgc ctgcgtccgg ccaggatgaa
     1081 ggtgtggacg cgcagatact tggtgagcgc ctcgtcgtcc tcgaactttc tgagcttggc
     1141 aaacggaatg accgcccccg cacgcatctc cacgcgcgtg ccgcgcttgt tgcagaactc
     1201 gcgcagcagc aggccggtgc gcaggcgcgg atgcaccagc ccggcggcgt ggaaaagcgc
     1261 gctgttcctg cctggaaaat acaccggcag caccgccgct cccgtccgcc gcaccagggc
     1321 cgccgcatgg ctgctccacg ccgcgtcctc aatgccgcgc cccgggcgcc agcgcgagac
     1381 ctcgcccgcc gggaagatga ccagcgcgcc gccgcccctg aggtggcgca tggcctcctt
     1441 catgccggcc aggttctccc tcgcggcctc cgcgccgccg aaggggttca ctggaatgat
     1501 gtgcggacgc agccgctcga acctgccgag cagcgagttg gtcatcacct tcacatccgg
     1561 acgctggcgc gccgcgtaat gcgccagcac caccggatcg aggatgccaa aggggtggtt
     1621 cgccacgatc accaacggcc cggatttcgg aaactcaaac tccggcgcct cgtccacatc
     1681 accactggcg tccatggcct ccagcacgga ttcgaaccag gcctcgcagg tccggccctc
     1741 actccgcgcg tcctgcaggc gcgccgcctc ctgataaatc tcctccagcc cgcgcagccc
     1801 cagcggcccc tcgaaaaagg ggctgaaagc acggtagagt ccgcgctgcc atgcggccgg
     1861 gagatgttcg ccggggtcga gaatcatcgg ttgatcactt tgtcaaagtg gcagcttgaa
     1921 gaattcacgc tggatggcaa cccctcatgc agtgccggtc ttgtcacttc ttcatgccgc
     1981 gtttgcacac cacttgcgct gaatttgccc ttgcaatccc gtccccggcc aggagctact
     2041 cgccgcatgt ccaaacccct gctcgaaacc ctgcacgccg gcgccggcta tctggaaaaa
     2101 cacggcgtgg aggaggcgcg gctgaacatg gagcatctgc tggcccatgt gctgggctgc
     2161 cgccgcctcg acctctacct gcgccacgcc gaggtcccgc gcgaggatga tctggcacgc
     2221 ctgcgcgagc tggccaagcg ccgtgcctca ggcgagccgc ttcagcacct gcttggcacg
     2281 gtggacttcc tcggccacga gctggtctgc gacgagcgcg ccctcatccc ccggccggag
     2341 acggagtatt tgtgcgatct gctcatcaag gcgagtcgcg ccgcgccgcc cgtccgcgtg
     2401 ctcgacatgg ccgccggctc cggctgcatc ggcctcaccc ttgcagccgc ctggcccggc
     2461 agccaggtga cgctggccga catctccgag gacgcgctcg atctggcgcg cctcaatgcc
     2521 ggacgcctcg gactcgatgc ggtgcgcatc gtgcgcagcg atctctttga gaaaatcagc
     2581 ggcggctttg acctcatcgt cagcaacctg ccctacatcc caagcgcgga gatcgccacc
     2641 ctcagccgcg aagtgcggcg cgaccccgcg ctcgccctgg atggcggccc ggatggtctg
     2701 gacatcgtgc ggcgtttcct cgccgccgcg cccgcgcacc tcaacgcgga gggcggcatc
     2761 gcccttgaca ccgcttccag ccgtgcacgc ctgcatggcg acaaacgaat ccaa
//
LOCUS       DAOMYJ010000409         2813 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Prosthecobacter sp. isolate AMR_MDS_5629
            NODE_13295_length_2813_cov_18.973532, whole genome shotgun
            sequence.
ACCESSION   DAOMYJ010000409 DAOMYJ010000000
VERSION     DAOMYJ010000409.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867850
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prosthecobacter sp. (wastewater metagenome)
  ORGANISM  Prosthecobacter sp.
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Verrucomicrobiaceae; Prosthecobacter.
REFERENCE   1  (bases 1 to 2813)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2813)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.30
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:34:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,742
            CDSs (total)                      :: 4,684
            Genes (coding)                    :: 4,676
            CDSs (with protein)               :: 4,676
            Genes (RNA)                       :: 58
            tRNAs                             :: 55
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2813
                     /organism="Prosthecobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13295_length_2813_cov_18.973532"
                     /isolate="AMR_MDS_5629"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1965333"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1589)
                     /locus_tag="PLP58_22455"
     CDS             complement(<1..1589)
                     /locus_tag="PLP58_22455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007416740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HRJ11359.1"
                     /translation="MTLPPLCALLCAPMLSVSNVSKSYAGRTLFSGVSFHINRGDKIG
                     LIGPNGAGKSTLFSLLLQESTPDEGLVTMEKGLDFGFLPQESAPVDDVTVLELATSHC
                     HEHNRWEAEPLAKRILSGLAFREGDFDRRARTLSGGWVMRAHLARLLVQEPDLLLLDE
                     PTNHLDLESLLWFQNYLRSYPGAILMISHDRAFLNALTDSILEIAHSKVHRYRGNYDD
                     YLREKAAREEQMVAAFENQQREIAKLQQWADRFKAKANFASRAQDKLKMIDRMERVDA
                     PQAAAKTVKFRFPQPPRSGQRVLSLKGVSFGYAETPVYEGLDFEVERGQRLVLVGPNG
                     AGKSTLLKLMAGALTPQAGKRELGHNVKAGYFAQYRGDVLNMRHTVLQSAMDLPSRPG
                     EQLTRTLLGSFLFHGDDVFKPVGVLSGGEKSRLALVRLLLDPPNLLLMDEPTTHLDMG
                     SIDALAGALEDYEGTLVFISHDVYFIRAMARGVLHIAGGVLTPYAGDYQYYLDKTRAS
                     SEREALTASLGNSQPGAWAAAA"
     assembly_gap    2123..2222
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    2411..2510
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2511..2751
                     /locus_tag="PLP58_22460"
     CDS             <2511..2751
                     /locus_tag="PLP58_22460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ11360.1"
                     /translation="SERRPIHSERRPIHSERRPIHFECRPIYFECRPIGSENGSMHLQ
                     SSPPRLECDTPHPERHTPRLKMDSMSLGCDITLLA"
BASE COUNT          492 a          921 c          740 g          460 t
ORIGIN      
        1 gcggcagcgg cccaggctcc gggctggctg ttgcccaggc tggcggtcag cgcctcgcgc
       61 tccgaggacg cgcgcgtctt gtccaggtag tactggtagt cccccgcgta cggcgtgagc
      121 accccgccgg cgatgtgcag caccccgcgc gccatggcgc ggatgaaata aacatcatgg
      181 ctgatgaaaa ccagcgtccc ctcatagtcc tccagcgcgc ccgccagcgc gtcaatgctg
      241 cccatgtcca ggtgcgtcgt cggctcgtcc atgagcagca ggttcggcgg gtcgagcagc
      301 agccgcacca gcgccaggcg gctcttttcc ccgccgctga gcacgcccac gggtttaaag
      361 acatcatccc cgtggaagag gaagctgccc agcagcgtgc gcgtcagttg ctcccccggc
      421 cggctcggca ggtccatggc ggactgcagc accgtgtgcc tcatgttcag cacatccccg
      481 cggtattgcg cgaagtagcc cgccttcaca ttatgcccca gctcccgttt gcccgcctgc
      541 ggcgtcaagg ccccggccat gagcttcaag agcgtggact tccccgcccc gttcggcccc
      601 accagcacca ggcgctggcc gcgctccacc tcgaagtcca gcccctcata caccggcgtc
      661 tccgcgtagc caaaggacac ccctttgagc gagagcacgc gctgcccgct gcgcggcggc
      721 tgcggaaagc ggaacttcac cgtcttcgcc gccgcctggg gcgcgtccac ccgctccatg
      781 cggtcaatca ttttcagctt gtcctgcgcg cgcgaggcga agttcgcctt cgccttgaag
      841 cggtccgccc actgctgcag cttggcgatc tcccgctgct ggttttcaaa cgccgccacc
      901 atctgctcct cccgcgccgc cttctcccgc aggtaatcat catagtttcc ccggtagcga
      961 tgcaccttgg agtgcgcgat ctccaggatg gagtccgtca gcgcgttgag gaaggcgcgg
     1021 tcgtgcgaga tcatcagaat cgcccccgga tagctcctca ggtagttttg aaaccacagc
     1081 agcgactcca gatccaggtg gttcgtcggc tcgtccagca gcagcaggtc cggctcctgc
     1141 accagcaggc gcgccaggtg cgcgcgcatc acccagccgc cgctcagcgt gcgcgcgcgg
     1201 cggtcgaagt cgccctcgcg gaaggccagc ccgctcagga tgcgcttcgc cagcggctcc
     1261 gcctcccagc ggttgtgctc gtggcaatgg ctcgtcgcca gctccagcac cgtcacatca
     1321 tccaccggcg cgctctcctg cggcaggaag ccaaagtcca gccccttctc catcgtcacc
     1381 agcccctcgt ccggcgtgct ctcctgcagc agcagggaga aaagcgtgga cttccccgcc
     1441 ccgttcggcc cgatcagccc gatcttgtcc ccgcggttga tgtgaaaaga cacgccgctg
     1501 aacaaagtgc ggcccgcgta ggacttggag acattcgaga cagaaagcat gggggcgcag
     1561 agcagtgcgc agagtggcgg gagggtcaag gcgtcaagac agccgccttg tgaatgttcc
     1621 gcgctgccgg ccggctccgt cttcctgtca atcgggtcca tccggtcccc ccgctgcctg
     1681 gccgggagac aggatgaccc ggtgaaaagg tgaaaaagcc ccccccggcc gcccccccgg
     1741 cgcgcctttc ccccaaaacg tgctggaaaa cagccccggc agccgctcga accgcaacgg
     1801 cagcggctcg aattgcgatg aaagccgctc gaaccacaaa aatggcaaat cggaccaccc
     1861 caacagccgc tcaaatggac tgcctgctgg gtagagcggc tgcaacagta aaaatgatga
     1921 cgcactaagc ccatcggatg aaactcctag tccatctagc cgtcctttga caccatagga
     1981 tgccaatcag agtgcatcgg atggctggca aagcgcatcg gatggctggc aaagcgcatc
     2041 gcatgactgt caaagcgcat cgcatggctg tcaaagcgca tcgcatggct gtcaaagcgc
     2101 atcgcatggc tgtcaaagcg cannnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2161 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2221 nntcaaatgt tcaaattagc acgtcggatg gcagtccaag cgcatcggat gccagtacaa
     2281 gacactctga acaccatccg acccacttgt actgccgccc tatccacttg tactgccgcc
     2341 ctatccactc tgaacgccgc cctatccact tgtactgccg ccctatccac tctgaacgcc
     2401 gccctatcca nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2461 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn ctctgaacgc
     2521 cgcccgattc actctgaacg ccgcccgatt cactctgaac gccgcccgat tcactttgaa
     2581 tgccgcccga tttactttga atgccgcccg attggctctg aaaacggttc gatgcacttg
     2641 caatccagtc cgccgcgctt ggaatgtgat acgccgcacc cggagcgcca tacgccgcgc
     2701 ctaaaaatgg attcgatgag cttaggatgt gatattacac tcttggcatg acgaatgacg
     2761 gggtgcgtcg ccagaatgat gtaggccggg tgtggcgcag cccgcccggc ttt
//
LOCUS       DAOMYJ010000410         2768 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Prosthecobacter sp. isolate AMR_MDS_5629
            NODE_13610_length_2768_cov_18.891633, whole genome shotgun
            sequence.
ACCESSION   DAOMYJ010000410 DAOMYJ010000000
VERSION     DAOMYJ010000410.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867850
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prosthecobacter sp. (wastewater metagenome)
  ORGANISM  Prosthecobacter sp.
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Verrucomicrobiaceae; Prosthecobacter.
REFERENCE   1  (bases 1 to 2768)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2768)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.30
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:34:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,742
            CDSs (total)                      :: 4,684
            Genes (coding)                    :: 4,676
            CDSs (with protein)               :: 4,676
            Genes (RNA)                       :: 58
            tRNAs                             :: 55
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2768
                     /organism="Prosthecobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13610_length_2768_cov_18.891633"
                     /isolate="AMR_MDS_5629"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1965333"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..592
                     /locus_tag="PLP58_22465"
     CDS             <1..592
                     /locus_tag="PLP58_22465"
                     /inference="COORDINATES: protein motif:HMM:NF024829.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="HlyD family efflux transporter periplasmic
                     adaptor subunit"
                     /protein_id="HRJ11361.1"
                     /translation="QTRRRLEAEAARQRGQGKEALARIAALEAQAQGENEKRLELEIA
                     LAELRSPLDGVVMTPDVHLKTGGFLQAGETLAEVASVDAWDLRLTLPEADLSLLEEAL
                     AAGPVPVRCLLYTQSAREFQAVITEPGQISPVLEPGAEGGVFTVTLPRVEAPPELVPL
                     LRPGLTGRVRISLHERPAGVVFLRGFTRWLRMRWWL"
     gene            1262..2224
                     /locus_tag="PLP58_22470"
     CDS             1262..2224
                     /locus_tag="PLP58_22470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009958991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HRJ11362.1"
                     /translation="MNPPSSRRQFLQLAAATFAASATGARAQGKEPLFQISLAEWSLN
                     KGMFKRAGAEPLEHLDFCKIARGLGIDGVEYVNQMFPDKAQDKAYLDEMKARQDGEGV
                     KGLLIMVDREGNLGAPDAAAKAKTVENHLKWLDAAARLGCHSIRVNAASDPKLSYEEQ
                     MKHAAEGLHNLCMEADKRGLFVVVENHGGLSSNGMWLTGVMKLADHPRVGILPDFGNF
                     YTDRNKGELYNPYKGLREFMPWVKQAMSAKAYDWDTGAGKFYTEDRREGREMTLDFER
                     LIKIVVAAGYRGYIGIEYEGDKHPEMEGIKRTKQALEEIRALLA"
     gene            complement(2229..>2768)
                     /locus_tag="PLP58_22475"
     CDS             complement(2229..>2768)
                     /locus_tag="PLP58_22475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2817 domain-containing protein"
                     /protein_id="HRJ11363.1"
                     /translation="WGLLAWARENAAWLSAHPAVLLPCLNPRGLAANTRADHRGLDLN
                     RRFHLEDDDLSGPWRSLVRARSWRLALCLHEDYDAQGIYVYELGAHQGIHSRVLLENA
                     ARILPADPRRRIDGSVARGGVIRRRRLPLHLPGLPEAVVLHQLGCPLTLTFETPSEFG
                     LDDRVRAHAAFIPAALAGA"
BASE COUNT          527 a          880 c          955 g          406 t
ORIGIN      
        1 ccagacgcgc cgcaggctgg aggcggaggc cgcccgccag cgcgggcagg ggaaggaggc
       61 cctggcgcgc atcgccgccc tggaggccca ggcccagggg gaaaatgaga agcgcctgga
      121 gctggagatc gcgctcgccg agctgcgcag cccgctggac ggcgtggtca tgacgcccga
      181 cgtgcatttg aagacgggcg gctttttgca ggccggtgaa accctggccg aggtggcctc
      241 cgtggacgcc tgggacctgc gcctgaccct gccggaggcc gatctcagcc tgctggagga
      301 ggccctggcc gccggcccgg tgccggtgcg ctgcctgctc tacacacaga gcgcgcgcga
      361 gttccaggcc gtcatcacgg aaccggggca gatcagcccc gtgctggagc cgggggcgga
      421 gggcggcgtc ttcaccgtca ccctgccgcg ggtggaggcg ccgccggaac tcgtcccgct
      481 gctgcgtccc ggcctgaccg ggcgggtgcg catctccctg catgagcgcc ccgccggggt
      541 ggtcttcctg cgcggcttca cccgctggct gcgcatgcgc tggtggctct gacgctccgg
      601 cgccccgtga aatgccgaca atgcccaccg aaacacttgg gaaaccgtta aaatcccggt
      661 gatggcttcc gatggaaatg ggaggccgtt aaaatcccgg caatggcttc cgataaagat
      721 gggaggccgt tgaaatcccg gcaatggctt ccgataaaca tgggaggccg ttaaaatccc
      781 ggcaatggct tccgataaac atgggaggcc gctaaaatcc cggcaatggc ttccgatgga
      841 catgggaggc cgttaaaatc ccggcaatgg cttccgataa agatgggagg ccgttgaaat
      901 cccggcaatg gcttccgata aacatgggag gccgttaaaa tcccggcaat ggcttccgat
      961 aaacatggga ggccgctaaa atcccggcaa tggcttccga tggacatggg aggccgttaa
     1021 aatcccggca atggcttccg ataaacatgg gaggccgtta aaatcccggc agtggcttcc
     1081 gatggacatg ggaggccgtt caagcgccgg agtggggttt cgatggcggc gtgccctggg
     1141 cgggccgggt gtttccgcgc ggttttgcag tggaggcgcc aataagcgcg tggattttgc
     1201 cattgccaga ggcggcggga tgggctgaat tcggaccgct ttggagcaac ccaaccgtct
     1261 tatgaatcca ccctcctccc gccgtcagtt cctgcaactc gccgccgcca cctttgccgc
     1321 ctcggccacc ggcgcgcgcg cccagggcaa ggagccgctt tttcaaatct ccctggcgga
     1381 gtggtcgctg aacaagggca tgttcaagcg cgcgggcgcg gagccgctgg agcacctgga
     1441 cttctgcaaa atcgcgcgcg gcctgggcat tgacggcgtg gagtatgtga accagatgtt
     1501 ccccgacaag gcgcaggaca aggcctacct cgacgagatg aaggcgcgcc aggatggcga
     1561 gggggtgaag ggcctgctga tcatggttga ccgcgagggg aacctgggcg cgcccgacgc
     1621 cgcggcgaag gccaagacgg tggaaaacca cctcaaatgg ctcgacgccg ccgcccgcct
     1681 gggctgccac agcatccgcg tgaacgcggc cagcgacccc aagctcagct acgaggagca
     1741 gatgaaacat gccgccgagg gcctgcacaa cctgtgcatg gaggcggaca agcgcggcct
     1801 ctttgtcgtg gtggagaacc acggcggcct ctccagcaac ggcatgtggc tcaccggcgt
     1861 gatgaaactg gccgaccacc cgcgggtggg cattttgccg gatttcggca acttttacac
     1921 cgaccgcaac aagggcgagc tctacaaccc ctacaagggc ctgcgcgagt tcatgccctg
     1981 ggtgaagcag gccatgagcg ccaaagccta cgactgggac acgggcgcgg gcaaattcta
     2041 caccgaggac cggcgcgagg ggcgggagat gacgctcgac ttcgagcgcc tgatcaagat
     2101 cgtcgtggca gcgggctacc ggggctacat cggcatcgaa tacgagggcg acaagcaccc
     2161 ggagatggag ggcatcaaac gcaccaagca ggcgctggag gaaatccgcg cgctgctggc
     2221 ctgaggcgtc acgccccggc cagcgccgcc gggatgaaag ccgcgtgcgc ccgcacccgg
     2281 tcatccagcc cgaactcgga gggcgtctcg aaagtcagcg tcagcggaca gccgagctgg
     2341 tgcagcacca ccgcctcggg cagccctggc agatgcagcg gcagccggcg gcggcggatg
     2401 accccgccgc gcgcgacaga cccgtcaatg cgccggcgcg ggtccgccgg cagaatgcgg
     2461 gcggcatttt ccagcaggac ccggctgtga atgccctggt gagcgcccag ctcatagacg
     2521 tagatgccct gcgcgtcgta gtcctcatgc agacaaaggg ccagcctcca ggagcgcgcg
     2581 cggaccaggc tgcgccaggg accgctcagg tcgtcatcct caagatgaaa acggcggttc
     2641 aaatccagcc cgcgatggtc cgcgcgcgtg ttggcggcca ggccgcgcgg attcaagcag
     2701 ggcagcagga cggcggggtg ggcggacagc caggccgcgt tttcccgcgc ccaggccagc
     2761 agccccca
//
LOCUS       DAOMYJ010000411         2747 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Prosthecobacter sp. isolate AMR_MDS_5629
            NODE_13762_length_2747_cov_16.230312, whole genome shotgun
            sequence.
ACCESSION   DAOMYJ010000411 DAOMYJ010000000
VERSION     DAOMYJ010000411.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867850
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prosthecobacter sp. (wastewater metagenome)
  ORGANISM  Prosthecobacter sp.
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Verrucomicrobiaceae; Prosthecobacter.
REFERENCE   1  (bases 1 to 2747)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2747)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.30
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:34:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,742
            CDSs (total)                      :: 4,684
            Genes (coding)                    :: 4,676
            CDSs (with protein)               :: 4,676
            Genes (RNA)                       :: 58
            tRNAs                             :: 55
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2747
                     /organism="Prosthecobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13762_length_2747_cov_16.230312"
                     /isolate="AMR_MDS_5629"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1965333"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..517
                     /locus_tag="PLP58_22480"
     CDS             <1..517
                     /locus_tag="PLP58_22480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ11364.1"
                     /translation="APAAPRPPGAPPVPGAPRTETGATTAPLTKTMPMKPMAAPRPPG
                     GAGTGPMSGASGGLPKATVKLQQTQPMQRPAIGAPPSAPVKRAAAADSQQFYEETDPD
                     AGLMPLSVVCFLLSLGLLAVQMLGGDIVKSEDNSPIMVPYSQPAKWETYNKEAGTWTN
                     RFASQLPVLPQ"
     gene            910..1296
                     /locus_tag="PLP58_22485"
     CDS             910..1296
                     /locus_tag="PLP58_22485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ11365.1"
                     /translation="MLKKNWYLLLPLLLVALPGLLFGYYTLSYGYGPKDAWNATRYFL
                     QSGTRYTQKYEEYNFSRLRNGTDGRSVYQIMKMQPFERHDNDSRWIYSLPKPGSTAYH
                     ERVVLLQRDKDNVPRVKGLVRGFKIP"
     gene            1321..1794
                     /locus_tag="PLP58_22490"
     CDS             1321..1794
                     /locus_tag="PLP58_22490"
                     /inference="COORDINATES: protein motif:HMM:NF013630.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HRJ11366.1"
                     /translation="MIHPRLLLAAFPALLAAGLSCSSGVRGVPRNLPVINIHDTPRTP
                     SHGMSKKDYPFHPDTGDYITAWAAVGEAAATERDIERWSSSHGGTISKKQPSPVRKAP
                     SKSSSKGGSTTYTIKPGDTLGGIAAKHKTTVAKIKAANGLKSDLIRAGKTLRIPR"
     gene            1816..>2747
                     /locus_tag="PLP58_22495"
     CDS             1816..>2747
                     /locus_tag="PLP58_22495"
                     /inference="COORDINATES: protein motif:HMM:NF014500.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HRJ11367.1"
                     /translation="MTSLLLVALGLAIVLLALIRLRLHPFLAMILAAFAVAFATPRAA
                     LEGQAAAQVEAGKITARQGAEFAASKAPDRVLAAFGDTCGKMGLLIAMASILGKCLLD
                     SGAAMSIVRAVLRLFGQARVHLGFILTGFGLAIPVYFDAVFYLLMPIGKAMARVTGKN
                     YLLYILTIVAGATMAHSLVPPTPGPLFAARELGVDISTMMLAGSITGLFTASTGLLWA
                     LWANRKWSIPLPVDTPDEVLAEDHPLPPLWLSLLPIVLPVALIAWGTAVSPLWFTDSN
                     VALSLGAVAGIFLLARHRPAGSASVHDAVQAAILS"
BASE COUNT          446 a         1108 c          711 g          482 t
ORIGIN      
        1 agcgcctgcg gcgccgcgtc caccgggagc gccgcccgtc cccggagctc cgcgcaccga
       61 gactggagcc accacggcac cgctgaccaa gaccatgccc atgaaaccca tggcggcacc
      121 gcgtccgccc ggcggcgctg gcaccggccc catgtcgggc gccagcggcg gcctgccaaa
      181 ggccaccgtc aagctccagc aaacccagcc gatgcagcgg cccgccatcg gcgcaccgcc
      241 cagcgcgccg gtgaaacgcg ccgccgccgc cgactcccag cagttttatg aggagactga
      301 cccggatgcc ggcctgatgc cactgtccgt ggtgtgcttc ctcctctcgc ttggcctgct
      361 cgccgtgcag atgctcggtg gagacatcgt caaatccgag gacaactcgc ccatcatggt
      421 gccctattcg caacccgcca aatgggaaac ctacaacaag gaggccggca cctggacgaa
      481 ccgcttcgcc agccagctcc ccgtgctgcc gcagtgatcc gcgccggttt cgaaccatta
      541 tttcaacacc ctaacccatt ctccgcacat gctttttgcc tggcttgtct tccttctctc
      601 cgtcgccgct ctcgctctga acttcattgc caaaagcggc ggcgcgggtc tttgattcca
      661 ccggtcatct ccatgaccgc gcgcggcctt cacggggatg ccacgtgaag gccgcttctt
      721 tttgacgttg gagttcaagc ttcagcttgt tccggaaaag ttccgcagcg gagccgcatc
      781 tcatgtcgcc tgaaggcttc actccaacgg ttttcaatcc ggcaaccgct cccgcatcca
      841 gtccatcttt catcttgcca gtgcccgccg ggtggcacag catcaaagcc gtttcccact
      901 cctcccgtca tgctcaagaa aaactggtac ctcctcctgc cattgctcct cgtcgccttg
      961 ccggggctgc tttttggcta ctacaccctg agctacggct acggcccgaa ggacgcctgg
     1021 aacgccacgc gctacttcct ccagtccgga acccgctaca cgcagaaata cgaggagtac
     1081 aacttctccc gcctcaggaa cggcaccgac gggcgcagcg tctatcaaat catgaagatg
     1141 cagcccttcg aacgccacga caacgactcc cgctggatct actccctgcc caaacccggc
     1201 tccaccgcct atcacgagcg cgtcgtgctt ctgcagcgtg acaaggacaa tgtgccccgc
     1261 gtgaagggcc tcgtgcgcgg cttcaaaatc ccctgaccgg cggcagccgc cctcctcttg
     1321 atgatccacc cccgcctcct cctcgccgca ttccccgccc tgctcgccgc cggcctctcg
     1381 tgcagctccg gcgttcgcgg cgtgccgcgc aacctgccgg tcatcaacat ccatgacacg
     1441 ccgcgcacac cgtcgcatgg catgtcgaaa aaagactacc ccttccatcc cgacaccggc
     1501 gactacatca ccgcctgggc cgccgtcggc gaggccgccg cgaccgagcg agacattgag
     1561 cgctggagca gctcccacgg cggcaccatc tccaaaaagc agccctcccc ggtgcgcaag
     1621 gcgccctcca agtcctcatc caaaggcggc tccaccacct acaccatcaa gcccggcgac
     1681 accctcggcg gcatcgccgc gaagcacaag accaccgtcg ccaaaatcaa ggccgccaac
     1741 ggcttgaagt cggacctcat ccgcgccggc aaaaccctca gaatcccgcg ctgacgcgcc
     1801 ttccccaggc tgcccgtgac ctccctcctg ctggttgccc tcggcctcgc catcgtcctg
     1861 ctcgcgctca tccggctgcg cctgcatccc tttctggcga tgatcctcgc cgccttcgcc
     1921 gtcgccttcg ccacgccgcg cgccgccctg gagggccagg ccgccgccca ggtggaggcc
     1981 ggaaagatca ccgccaggca gggcgcggag ttcgccgcct ccaaggcgcc cgatcgcgtg
     2041 cttgccgcct ttggcgacac ctgcggaaag atgggcctgc tcatcgccat ggcctccatc
     2101 ctcggcaagt gcctgctcga ctccggcgcc gccatgagca tcgtccgcgc cgtgctccgc
     2161 ctcttcggcc aggcgcgcgt ccacctcggc ttcatcctca ccggtttcgg cctggccatc
     2221 cccgtgtatt tcgacgccgt cttctacctg ctcatgccca ttggcaaagc catggcgcgc
     2281 gtcacgggga aaaactacct cctctacatc ctcaccatcg tcgccggagc caccatggcg
     2341 cactccctcg tgccgcccac acccggcccg ctttttgccg ccagggagct cggcgtggac
     2401 atctccacca tgatgctcgc cggctccatc accggactct tcaccgcctc caccggcctg
     2461 ctctgggcct tgtgggcgaa ccgcaaatgg agcatccccc tgcctgttga caccccggac
     2521 gaggtgctgg ctgaagacca tcccctgccc ccgctctggc tttccctcct gcccatcgtc
     2581 ctgcccgtgg ccctcatcgc ctggggcacc gccgtcagcc ccctgtggtt cactgacagc
     2641 aatgtcgccc tctccctcgg cgccgtggcc ggcatcttcc tcctcgcgcg ccaccggccc
     2701 gccggctccg cctccgtcca cgacgccgtg caggccgcca tcctctc
//
LOCUS       DAOMYJ010000412         2739 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Prosthecobacter sp. isolate AMR_MDS_5629
            NODE_13811_length_2739_cov_16.605812, whole genome shotgun
            sequence.
ACCESSION   DAOMYJ010000412 DAOMYJ010000000
VERSION     DAOMYJ010000412.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867850
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prosthecobacter sp. (wastewater metagenome)
  ORGANISM  Prosthecobacter sp.
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Verrucomicrobiaceae; Prosthecobacter.
REFERENCE   1  (bases 1 to 2739)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2739)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.30
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:34:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,742
            CDSs (total)                      :: 4,684
            Genes (coding)                    :: 4,676
            CDSs (with protein)               :: 4,676
            Genes (RNA)                       :: 58
            tRNAs                             :: 55
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2739
                     /organism="Prosthecobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13811_length_2739_cov_16.605812"
                     /isolate="AMR_MDS_5629"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1965333"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..879
                     /locus_tag="PLP58_22500"
     CDS             <1..879
                     /locus_tag="PLP58_22500"
                     /inference="COORDINATES: protein motif:HMM:NF024770.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HRJ11368.1"
                     /translation="APAHGRHRLVRAGAGGALKAGRKGRVGVLSSLALEQLPPQTALL
                     VDEMRRALAASGCALVFHHVKGCFSGQPARALEKLAAQETADLWIVLGSREPMQRWFL
                     RRGLPCLVVGSCLPEVALPSMDADHRAVCRHAGHLLLRHGRKRIALVRHKDHYGGEDE
                     SEHGLREALAENARLQVLRHDGSREHVCALVDKALRAEPPPDAFLVCRAVHALTVLTH
                     LMRRGVRVPADAALISRDDHPALRATSPVVTCYTLDARRFARRVVLAVRRYVEGGALP
                     AQGARLMPEFCPGETV"
     gene            956..1040
                     /locus_tag="PLP58_22505"
     tRNA            956..1040
                     /locus_tag="PLP58_22505"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:990..992,aa:Leu,seq:cag)
     gene            1064..1735
                     /locus_tag="PLP58_22510"
     CDS             1064..1735
                     /locus_tag="PLP58_22510"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012419109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone/menaquinone biosynthesis
                     methyltransferase"
                     /protein_id="HRJ11369.1"
                     /translation="MQDPGFVRKAFAGIAGRYVLANHVLSLGIDILWRRRAAAKVAAR
                     GPGMILDLATGSGDLAVEMQRRCPRALIIGADFSQPMMRRAQERNFHQLITADGTRLP
                     FQDAVFDAVTVAFGLRNMASWPGALAEMRRVLKPGGMLLILDFSRPRAPLVRQGYGFY
                     LKHVMPRVAGLITGQRAAYEYLCSSVERFPSGPEMEALIRSCGFAETRAEPLSFGIAT
                     LYECW"
     gene            complement(1750..1824)
                     /locus_tag="PLP58_22515"
     tRNA            complement(1750..1824)
                     /locus_tag="PLP58_22515"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1790..1792),aa:Met,seq:cat)
     gene            complement(1831..1905)
                     /locus_tag="PLP58_22520"
     tRNA            complement(1831..1905)
                     /locus_tag="PLP58_22520"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1871..1873),aa:Asn,seq:gtt)
     gene            2126..>2739
                     /locus_tag="PLP58_22525"
     CDS             2126..>2739
                     /locus_tag="PLP58_22525"
                     /inference="COORDINATES: protein motif:HMM:NF013739.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HRJ11370.1"
                     /translation="MNREQPSIVIVGAGLSGLACARLLARAGLGFKIYEAADAVGGRV
                     CSERVEGFTLDRGFQVFLPAYPEAKRVLDYDGLKLRPFYRGAEVYYKGRLHRLADPFS
                     HPRDAIRHARDPFVSWKDKWLSLVLRKEVFCTRQVERRLPEMQTLKYLRDFGFSDDFI
                     DRFFRPFFGGVFLERDLRTSARMFLFLYSMFGQGGAALPAHGMQA"
BASE COUNT          403 a          820 c          986 g          530 t
ORIGIN      
        1 gcgccggcgc acggccggca caggctcgtc cgcgcgggtg ccggtggcgc gcttaaagcc
       61 gggcggaagg gcagggtggg cgtgctctcc agtctggcgc tggagcaact gccgccgcag
      121 acggcgctgc tggtggacga gatgcggcgc gcgctggccg cgtccggctg cgcgctggtt
      181 tttcatcatg tgaagggctg cttttccggc cagccggcgc gggctttgga gaagctggcg
      241 gcccaggaga ccgcggacct gtggatcgtg ctcggctcgc gcgagccgat gcagcgctgg
      301 ttcctgcgcc gggggctgcc gtgcctggtg gtggggtcgt gcctgccgga ggtggccctg
      361 ccctcgatgg acgcggacca ccgcgcggtg tgccgccatg ccggtcatct gctgctgcgg
      421 catgggcgga aaaggatcgc gctggtgcgg cacaaggacc actacggcgg cgaggatgag
      481 agtgagcatg gattgcgcga ggcgctggcg gaaaacgcgc ggctgcaggt gctgcggcat
      541 gacggcagcc gggagcatgt gtgcgcgctg gtggacaagg cgctgcgcgc cgaaccgccg
      601 cccgacgcct ttctggtctg ccgggccgtc cacgcgctga cggtgctgac gcatctgatg
      661 cggcgcggag tgcgggtgcc cgcggacgcg gccctgatca gccgggacga ccatcccgcg
      721 ctgcgcgcca cgagtccggt cgtcacctgc tacacgctgg atgcccgccg cttcgcccgc
      781 cgtgtggtgc tggcggtgcg gcgttatgtg gagggcggcg cgctgcccgc gcagggcgcg
      841 cggctgatgc cggagttctg ccccggggag acggtgtgag gcacgggaaa atcccgtttt
      901 ttccggttga gttccgccgc cgcccggcca gtattgcggc ccgttcaatg gcaatgcgcg
      961 cgtggcggaa ttggtagacg cgctagattc aggttctagt gagtaacatc gtggaggttc
     1021 gagtcctctc gcgcgcacca tcccagatca tcggggtggc accatgcaag acccaggatt
     1081 tgtcagaaaa gccttcgccg gaatcgccgg acgttatgtc ctggccaatc atgtgctgag
     1141 cctgggcatt gacatcctct ggcggcggcg cgcggcggcg aaagtggcgg cgcgcggtcc
     1201 gggcatgatt ctggacctgg ccacgggcag cggtgatctg gccgtggaga tgcaaaggcg
     1261 ctgcccgcgg gcgctgatca tcggcgccga tttttcccag ccgatgatga ggcgggcgca
     1321 ggagcggaac tttcatcagc tcatcactgc ggatggcacg cggctgcctt ttcaagacgc
     1381 ggtcttcgac gcggtgacgg tggctttcgg cctgcgcaac atggcctcat ggccgggcgc
     1441 gctggccgag atgcggcgtg ttttgaaacc gggcggcatg cttttgatcc tggatttctc
     1501 ccggccgcgc gcgccgctgg tccggcaggg atacggcttc tatctcaaac atgtgatgcc
     1561 gcgcgtggcg ggcttgatca ccgggcagcg cgcggcatac gagtacctgt gcagctcggt
     1621 ggagcggttt ccttcggggc cggagatgga ggcgctgatc cgctcgtgcg ggtttgcgga
     1681 gacgcgggcc gagcctttga gcttcggcat tgccactctg tatgagtgct ggtgaagacc
     1741 ggcgcaacct ggtggcagag gggagatttg aactcccgac caaaggctta tgagtcctct
     1801 gctctaccac tgagctactc tgccggaggt tggctcctga ggtagggttc gaacctacga
     1861 cctagtggtt aacagccacc cgctctaccg ctgagctact caggaatcac gtcgcccttt
     1921 cgggcaaaca ggagggcgag actattcggc ggccctgttc gcgcaagtga ttgtttgccg
     1981 cgcgcgagat tttcagcgcc tcattcccgg ccatgaaaac gtccgcgcgt tttgaaaaac
     2041 gccgccggtt tgccgtccac agagttcact cgcgcgagcc agccgcttgc aatcaaggaa
     2101 ctggcattca tggtggcgcc gcgtcatgaa tcgggagcag cccagcattg tcatagtcgg
     2161 ggcgggtttg tccggcctgg cttgcgcgcg gctgctggcg cgggcggggc tgggctttaa
     2221 aatttacgag gcggcggatg ccgtgggcgg gcgcgtgtgc tccgagcgtg tggagggctt
     2281 cacactggac cgcggctttc aagtgttcct gcccgcctac ccggaggcga aacgggtgct
     2341 ggactatgac gggctgaaac tgcggccttt ctaccggggc gcggaggtgt attacaaagg
     2401 caggctgcac cggctggcgg accccttcag ccatccgcgt gacgccatcc gccatgcgcg
     2461 cgatccgttt gtgtcctgga aggacaaatg gctctcgctg gtgctgagaa aagaggtctt
     2521 ttgcacgcgg caggtggagc ggcggctgcc ggaaatgcag accctgaaat atctgcggga
     2581 ctttggtttc tccgatgact tcattgaccg ctttttccgg ccttttttcg gcggcgtgtt
     2641 cctggagcgc gacctgcgca cctcggcccg catgtttttg tttctctact ccatgttcgg
     2701 ccagggcggc gcggccctgc cggcgcatgg catgcaggc
//
LOCUS       DAOMYJ010000413         2720 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Prosthecobacter sp. isolate AMR_MDS_5629
            NODE_13967_length_2720_cov_17.522702, whole genome shotgun
            sequence.
ACCESSION   DAOMYJ010000413 DAOMYJ010000000
VERSION     DAOMYJ010000413.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867850
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prosthecobacter sp. (wastewater metagenome)
  ORGANISM  Prosthecobacter sp.
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Verrucomicrobiaceae; Prosthecobacter.
REFERENCE   1  (bases 1 to 2720)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2720)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.30
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:34:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,742
            CDSs (total)                      :: 4,684
            Genes (coding)                    :: 4,676
            CDSs (with protein)               :: 4,676
            Genes (RNA)                       :: 58
            tRNAs                             :: 55
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2720
                     /organism="Prosthecobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13967_length_2720_cov_17.522702"
                     /isolate="AMR_MDS_5629"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1965333"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..991)
                     /locus_tag="PLP58_22530"
     CDS             complement(<1..991)
                     /locus_tag="PLP58_22530"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HRJ11371.1"
                     /translation="MNLLRTAAAACLVLALFHSPALGAERLNFLLVTTDDMSCDSVGA
                     YGCRLAGTTPHMDRLAARALRFNHAFVQVGNCFPSRNVMWSGRYPHTTRVEGFYQVKD
                     PDYPHLSDLMRAGGWFTGIRGKVSHSTPYSPYPGWDADLDTLPDGTRAHIKDARSYFL
                     STADGIARAKAAGKPFCLNINISDPHKPFYAEGGRADPHVPSRVFTAEEAPVPGFLFD
                     DPQVRAELAEYYSSVRRADDCLGEVLRALEESGEAGRTLVLFLSDHGMPLPFAKTQVY
                     FHSTRTPLMVLWPGVTRAGAVDDEHLVSAVDLTPTLLEAAGLPAPAGTQGRSFA"
     gene            1253..1792
                     /locus_tag="PLP58_22535"
     CDS             1253..1792
                     /locus_tag="PLP58_22535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ11372.1"
                     /translation="MSQSQSIGSVDIPEDHLAVDFAVLEHYQKVSSELLRISLLGIPV
                     VGYMATEDKIPEGIFAHSGILISTIASLCCFALSASLALLHRYVSSDGMSDHIQLLRL
                     EALGKMEGTDKIEKRRQKRNTAFKISSLALAFSAIFLALAAAALVLAISLTLKNIGRS
                     QSIEADKTMVEPSANTEMK"
     gene            1908..>2720
                     /locus_tag="PLP58_22540"
     CDS             1908..>2720
                     /locus_tag="PLP58_22540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009964001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HRJ11373.1"
                     /translation="MKPADSRPPAESLRGVVERVLFHTEETGFCILRVVPQERREAVG
                     LIGRAPRVVAGEEFHASGVWENSREHGRQFKAETLRLTRPDSPEGIERYLGSGLIEGI
                     GPAYARRMVERFGKGVFEIIEHESAKLEEVEGIGRKRRLEIRESWLRQRSIHKIMIFL
                     HEHGVSSARALRIYKTYGEEAVAVLKANPYRLAQDIHGIGFKTADQIARQLGMAEDAP
                     ERVRAAVLHTLSAAASGGHSCLPESKAVEQTMQLVGAGAEAVSAALETLLAEQ"
BASE COUNT          538 a          812 c          885 g          485 t
ORIGIN      
        1 gcgcgaagct gcgcccctgc gtgcccgccg gcgccggcag ccccgccgcc tccagcagcg
       61 tcggcgtcag gtccaccgcc gagaccagat gctcgtcatc caccgcgccc gcccttgtca
      121 cccccggcca cagcaccatc agcggtgtgc gcgtgctgtg gaagtacacc tgcgtcttgg
      181 cgaagggcag cggcatcccg tggtccgaga ggaaaaggac cagcgtgcgc cccgcctccc
      241 ccgactcctc cagcgcgcgc agcacctccc ccaggcagtc atccgcgcgc cgcaccgacg
      301 agtaatactc cgccagctcc gcgcgcacct gcggatcatc aaaaagaaaa cccggcaccg
      361 gcgcctcctc cgccgtgaag acccggctcg gcacatgcgg gtccgccctg ccgccctcgg
      421 cataaaaagg cttgtgcgga tcggagatgt tgatgttcag gcagaagggc ttccccgccg
      481 ccttcgccct ggcgatgccg tccgccgtgc tcaggaaata cgagcgcgcg tccttgatgt
      541 gcgcccgcgt cccgtccggc agcgtgtcca ggtccgcatc ccagcccgga tacgggctgt
      601 aaggcgtgga gtggctcacc ttcccccgga tgcccgtgaa ccagcccccc gcccgcatca
      661 gatcactcaa atgcggataa tccggatcct tcacctggta aaagccctcc acccgcgtcg
      721 tgtgcgggta gcggcccgac cacatcacat tgcgggaggg gaagcaattg cccacctgca
      781 caaaagcgtg gttgaagcgc agcgcccgcg ccgccagccg gtccatgtgc ggcgtcgtcc
      841 ccgccagcct gcagccgtag gcccccaccg aatcacagct catgtcatcc gtggtgacga
      901 gcaggaaatt cagcctctcc gcgcccaaag cgggcgagtg gaaaagcgcg aggacgaggc
      961 aagcggcggc ggcagtgcgg agcaggttca tgcgcgtggg aacgcggaaa gcgggcgggc
     1021 atttcgcgag gtcctgccca tgacgagaaa atcttgatgc acttgatgcc tattcgtgcc
     1081 agcggcgtgt ccagccaaag ctctggcaga gcgctgaagc catccatcgc gatatgagag
     1141 tcagggcgtg gaagaaaagt taaacccaca cttgcagtac tgatgacatg tttgacagaa
     1201 ttaaaccgaa tcaaacaggt tctccggctt caataccact cgtcttccac ttatgagcca
     1261 aagccaatcc atcggatcag ttgacatacc tgaagaccat ctagctgtcg attttgcagt
     1321 attggaacat taccagaagg tctcgtcaga gttgcttcgc atttcactct tggggattcc
     1381 ggttgttggc tatatggcaa cagaagacaa gattcctgag ggaatttttg cccattcagg
     1441 cattttgatc agtacgattg cctcgctctg ttgcttcgcg ctttcggcat ccctggcatt
     1501 gctgcacaga tatgtgtcga gtgatggcat gtcagatcac atccaattgc tgcgccttga
     1561 ggcattgggg aaaatggagg gcaccgataa gatcgaaaag cgcaggcaaa aaagaaatac
     1621 agcgtttaaa atttccagtt tggcactggc attttcagcc atttttcttg ctcttgcggc
     1681 tgccgctttg gtcctggcca tatcgctgac tctaaaaaac attggccggt cgcagtcaat
     1741 tgaggcggac aagactatgg tggaaccttc cgccaacacc gagatgaagt gacccgtcaa
     1801 aagcgcaagt ttcaagcctg ttgagcgtgg tgatttggtc agctggtgct ggtgctttga
     1861 agcgtgatga caagcagaca caacgggaga atgaatccct cagacccatg aagcccgccg
     1921 actcacgccc gcccgccgag agcctgcgcg gggtggtgga gcgtgtgctg tttcacacgg
     1981 aggagacggg attttgcatc ctgagggtgg tgccgcagga gcggcgggag gcggtgggcc
     2041 tcatcgggcg agcgccgcgc gtggtggcgg gggaggagtt ccatgcctcg ggtgtgtggg
     2101 aaaattcgcg cgagcacggg cggcagttca aggcggagac actgcggctg acgcgcccgg
     2161 attcgccgga gggcatcgag cgttacctgg gcagcggctt gatcgagggc atcggtccgg
     2221 cgtatgcgcg gcggatggtg gagcggtttg gcaagggcgt gtttgaaatc atcgagcacg
     2281 agtcggcgaa gctggaggag gtggagggca tcgggcggaa gcggcggctg gagattcgcg
     2341 agtcgtggct gcggcagcgg agcattcaca agatcatgat cttcctgcac gagcacggtg
     2401 tcagctccgc gcgcgcgctg cgcatttaca agacctacgg ggaggaggcg gtggcggtgc
     2461 tgaaggcgaa cccttaccgg ctggcgcagg acattcacgg catcggcttc aagacggcgg
     2521 accagatcgc ccgccaactc ggcatggcgg aggacgcgcc ggagcgggtg cgcgcggcgg
     2581 tgctgcacac gctgtcggcg gcggcgagcg gggggcattc ctgcctgccg gagtcgaagg
     2641 cggtggagca gacgatgcag ctcgtggggg cgggtgccga ggcggtgagc gcggcgctgg
     2701 agaccctgct ggcggagcag
//
LOCUS       DAOMYJ010000414         2701 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Prosthecobacter sp. isolate AMR_MDS_5629
            NODE_14112_length_2701_cov_21.865835, whole genome shotgun
            sequence.
ACCESSION   DAOMYJ010000414 DAOMYJ010000000
VERSION     DAOMYJ010000414.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867850
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prosthecobacter sp. (wastewater metagenome)
  ORGANISM  Prosthecobacter sp.
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Verrucomicrobiaceae; Prosthecobacter.
REFERENCE   1  (bases 1 to 2701)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2701)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.30
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:34:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,742
            CDSs (total)                      :: 4,684
            Genes (coding)                    :: 4,676
            CDSs (with protein)               :: 4,676
            Genes (RNA)                       :: 58
            tRNAs                             :: 55
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2701
                     /organism="Prosthecobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14112_length_2701_cov_21.865835"
                     /isolate="AMR_MDS_5629"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1965333"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..561)
                     /locus_tag="PLP58_22545"
     CDS             complement(<1..561)
                     /locus_tag="PLP58_22545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ11374.1"
                     /translation="MKKILVALFCLAALVLSAAGAASWWVMRQMGPDIWVQQAEKNWN
                     CRAQIDEASLNLLTSPATLVFKGVHLARRDAEADKPLPERARMEAATADIYIAELRLE
                     VRLEDLLKKRVHVENLAIIEPVIRETQTPDGSTLEALFKKPRSEKTTSNAAAAATANT
                     APSPAPPPASAPAAPEQSLPLDEDPAA"
     gene            complement(613..1203)
                     /locus_tag="PLP58_22550"
     CDS             complement(613..1203)
                     /locus_tag="PLP58_22550"
                     /inference="COORDINATES: protein motif:HMM:NF024811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HRJ11375.1"
                     /translation="MLQIPEHPFRAFIFDCDGTLVDSMPLHFIAWTQSLRAHDAPFDF
                     TEEFFYSTAGVREHDMVRRLNDMHGCALDPDGVAETKAEIFIKLVPQVPVVRPVADFA
                     RSLHGRAPMAVASGSEEPIVRACLEANELILLFETIITPRDVAKGKPAPDMFLLAAER
                     LAVPPEDCLVLEDGMAGVEAAKAAGMQWVFIPRTLR"
     gene            1347..>2701
                     /locus_tag="PLP58_22555"
     CDS             1347..>2701
                     /locus_tag="PLP58_22555"
                     /inference="COORDINATES: protein motif:HMM:NF013740.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HRJ11376.1"
                     /translation="MKNLPTVFQRNTLWTAVTALSVTVIGALAIGFVYLGTQVIAFLQ
                     PILTPFAVAGVLAYLLEPGVEWLERKGMSRQSSVLVAFAVFSLAIFGLAWWVVPKVSG
                     QTAALVKKVPAYTVTASKAVLEFSTRIEKEYGIELIPHTTARELEADLKKAESAPAPV
                     EASTQPAASAPAAGATTPVDVAPEAAGTGALEEFDFKALLTGEWVRTALPTVLANAWK
                     VVKSTFGGFLGVFGFLLSLIIVPLYLYYFLIESAKIQEQWSDYLPLRASRFKDEVVDC
                     LNEINGYLIAFFRGQLFVSFINGIATGIGLVVIGLDFGLLIGLALCVLGIIPYLGIAL
                     CWIPAVIIGAVQGGSALIPGDPWWAMPLAVTAIFIVVQQVDGFFITPRIVGEAVGLHP
                     MTVIASVLVWTLLLGGLLGAILAVPMTASVKVLFQRYIWRARRRRRLRPHLRRLLRHV
                     LR"
BASE COUNT          422 a          853 c          915 g          511 t
ORIGIN      
        1 cgcggcgggg tcttcgtcca gcggcaggga ttgctccggc gcggctggcg cggaagcggg
       61 cggcggggca ggggaagggg cggtgtttgc ggtggcagcg gcggccgcgt tggatgtggt
      121 tttttcagag cggggctttt tgaaaagcgc ctcaagagtg ctgccgtcgg gagtttgggt
      181 ctcgcggatg acgggttcga tgatggcgag attttcgacg tggacgcgct ttttgagcaa
      241 gtcctccagc ctcacttcga ggcgcagctc ggcgatgtaa atgtcagccg tggcggcttc
      301 catgcgggcg cgctccggga ggggcttgtc cgcctcggcg tcgcgccggg ccaggtggac
      361 gcctttgaag acgagggtgg ccgggctggt gaggaggttc agcgaggctt cgtcaatctg
      421 ggcgcggcag ttccagttct tctccgcctg ctggacccag atgtccggcc ccatctggcg
      481 catgacccac catgaggcgg ctcccgcggc ggagagcacg agggcggcca ggcagaacag
      541 agcgacaagg atctttttca tggcgggtca tggtgccgcc gcgccgcatg aacgcaagac
      601 gcaatcgaac cctcagcgca gcgtgcgcgg gatgaacacc cactgcatgc cggccgcttt
      661 cgccgcctcc acgccggcca tgccatcctc cagcaccagg cagtcctccg gcggcactgc
      721 aaggcgctcg gcggccagca ggaacatgtc cggggcgggt ttgcctttgg cgacatcacg
      781 cggagtgatg atggtttcaa acaggaggat caactcgttc gcctccaggc aggcgcgcac
      841 gatgggctcc tcgctgccgg aggccacggc catgggcgcg cgcccgtgca gcgagcgggc
      901 gaagtcggcc accgggcgca cgacgggcac ctgcggcacg agcttgatga aaatctccgc
      961 cttggtctcc gccacgccgt cggggtcgag cgcgcatccg tgcatgtcat tgaggcggcg
     1021 caccatatca tgctcgcgca cgccggcggt ggaatagaaa aactcctcgg tgaagtcgaa
     1081 aggcgcgtcg tgcgcccgca ggctctgcgt ccaggcgatg aaatgcagag gcatggagtc
     1141 aacgagggtg ccgtcgcagt cgaagatgaa ggcgcggaag ggatgttcgg ggatttgcag
     1201 catgggggga tgcgccgact tttaagcggc ggacgcgggc cgtaaaggcg caaggcgccc
     1261 gccgcgcgcg cgtccccatg cgcgaactgc aaaatcagcg gaaaattcca gttccacccg
     1321 gcgcgccccg ccgctatagc cctgccatga aaaatctgcc cacggtcttt cagcgcaaca
     1381 ccttgtggac ggcggtcacg gcgctgtccg tcaccgtcat tggcgcgctt gccatcggct
     1441 ttgtgtatct gggcacgcag gtcatcgcct ttttgcagcc gatcctcact cccttcgcgg
     1501 tggccggcgt gctggcctac cttctggagc cgggcgtcga gtggctggag cgcaagggca
     1561 tgagccggca gtcctccgtg ctcgtggcct tcgcggtttt ctcgctggcc atcttcgggc
     1621 tggcctggtg ggtggtgccg aaggtgtccg gccagacggc ggcactggtg aagaaagtgc
     1681 cggcctacac cgtgacggcg agcaaggccg tgctggaatt ctccacccgc atcgaaaaag
     1741 agtacggcat cgagctgatc ccgcacacga ccgcgcgcga gctggaggcg gatctgaaga
     1801 aagctgaaag cgcccccgct cctgtcgagg catccacgca gcccgccgcc agcgctcctg
     1861 ctgccggtgc gacaacccca gttgatgtcg cgcccgaagc agccggcacg ggggccctgg
     1921 aggagttcga cttcaaagcg ctgctgactg gcgagtgggt gcgcaccgct cttcccacgg
     1981 tgctggcgaa cgcctggaag gtagtcaagt ccaccttcgg cggcttcctc ggtgtcttcg
     2041 gcttcctgct ctcgctcatc atcgtgccgc tctacttgta ttacttcctc atcgagagcg
     2101 cgaagatcca ggagcaatgg tccgactacc tgccgctgcg cgccagccgc ttcaaggacg
     2161 aggtggtgga ctgcctcaac gagatcaacg gctacctcat cgccttcttc cgcgggcagc
     2221 tttttgtcag tttcatcaat ggcatcgcca ccggcatagg cttggtggtc atcggcctgg
     2281 acttcgggct gctcatcggc ctggctctgt gcgtgctggg catcattccg tatctcggca
     2341 tcgccctgtg ctggatcccc gccgtcatca tcggggccgt gcagggcggc agcgcgctca
     2401 ttcccggtga tccgtggtgg gccatgccgc ttgccgtcac ggccatcttc atcgtcgtgc
     2461 agcaggtgga cggtttcttc atcacgccgc gcatcgtcgg cgaggccgtg gggctgcatc
     2521 caatgacggt catcgccagc gtgcttgtgt ggacgcttct gctcggcggt ctgctcggcg
     2581 ccatccttgc cgtgcccatg accgccagtg tgaaggtgct ctttcagcgc tacatctggc
     2641 gcgcgcgtcg gcggcgtcgg ctccggccac atctccgccg attattacgg catgtattgc
     2701 g
//
LOCUS       DAOMYJ010000415         2687 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Prosthecobacter sp. isolate AMR_MDS_5629
            NODE_14240_length_2687_cov_22.523556, whole genome shotgun
            sequence.
ACCESSION   DAOMYJ010000415 DAOMYJ010000000
VERSION     DAOMYJ010000415.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867850
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prosthecobacter sp. (wastewater metagenome)
  ORGANISM  Prosthecobacter sp.
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Verrucomicrobiaceae; Prosthecobacter.
REFERENCE   1  (bases 1 to 2687)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2687)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.30
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:34:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,742
            CDSs (total)                      :: 4,684
            Genes (coding)                    :: 4,676
            CDSs (with protein)               :: 4,676
            Genes (RNA)                       :: 58
            tRNAs                             :: 55
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2687
                     /organism="Prosthecobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14240_length_2687_cov_22.523556"
                     /isolate="AMR_MDS_5629"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1965333"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..743
                     /locus_tag="PLP58_22560"
     CDS             <1..743
                     /locus_tag="PLP58_22560"
                     /inference="COORDINATES: protein motif:HMM:NF012264.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HRJ11377.1"
                     /translation="MLAAAPLDAARQLALAPVFASAGPVELEKLLPLLRKAKESDTAR
                     ALATELAKNPAIASQQESLYRTALHQHPPEIFETIILPAYTRASEAQEEKKRALPALA
                     EKAVATGRATEGRHVFESGKGTCIACHQIGGVGRALGPDLSKIGSIRTERDLIESILF
                     PSASLARDYETHLIETADGQTVIGVIRSHTAEGLLVMDVAGQEKNLPHAAIAASTQLP
                     ASLMPMGLDQTLPEQDLLDLVAWLRSLK"
     gene            1038..2396
                     /locus_tag="PLP58_22565"
     CDS             1038..2396
                     /locus_tag="PLP58_22565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009964693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HRJ11378.1"
                     /translation="MSTPSDRRSFLKHSALAAAAAGFPAVVRSASPNSMLQVASIGAD
                     GMAFSDIKNIGAHGKVRYVGFCDIDSTRFAKADAAHPGVPHFADFREMLASLGDKVDA
                     VNVGTPDHMHAKAAIEAMRRGKHVYCQKPLAHTVWESRQMRLEAEKAGVVTQMGNQIH
                     SNLEYRLGTRLLKEGAIGKIKEVYSWVGVTGNERNKKLEPLPGAPVPAHVNWDLWIGV
                     APMREYAPCYHPFVWRDWQDFGGGALGDFGCHILDPVFTALGLNAPLTVTARNSGINA
                     HIWPVNEEVEYVFPGNALTADKTLKVTWSDGGLRPDRKKAKMPPDLELPRSGSLFIGE
                     EGNMVLAHVAGPRLYPLEKFQGFAYPKEEGKSHWHVWVDACLAGQKTSDGFHYAGPLS
                     EAVQLGNVATRFCTRGPIDSRTGDPLNPVILEWDAEKLAFTNAPEANPLLTKTYREGW
                     AI"
     gene            complement(2427..>2687)
                     /locus_tag="PLP58_22570"
     CDS             complement(2427..>2687)
                     /locus_tag="PLP58_22570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009962799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HRJ11379.1"
                     /translation="SSDLGPGQPLAPLAAFALECAHPHDLVEYANTRGLALRGGHHCT
                     QPLMRRFKVPGTSRASFYFYNTEQEVDAMLGILRQAVEFFGG"
BASE COUNT          492 a          959 c          859 g          377 t
ORIGIN      
        1 ccatgctcgc cgccgcgccc ctggatgccg cccggcaact cgcgctcgcc cccgtcttcg
       61 ccagcgccgg ccccgtcgaa ctcgaaaagc tcctccctct cctgcgcaaa gcgaaggaaa
      121 gcgacaccgc ccgcgccctc gccaccgagc tggccaaaaa ccccgccatc gccagccagc
      181 aggagagcct ctaccgcacc gccctccacc agcacccgcc cgagattttt gagaccatca
      241 ttctccccgc ctacacccgc gccagcgaag cccaggagga gaaaaagcgt gccctgcccg
      301 ccctcgccga aaaagccgtc gccacaggcc gcgccaccga gggcaggcac gtcttcgagt
      361 ccggcaaagg cacctgcatc gcctgccacc agatcggcgg cgtgggccgc gccctcggac
      421 ccgacttgtc aaagatcggc tccatccgca ccgagcgcga cctcatcgag agcatcctct
      481 tccccagcgc ctccctcgcc cgcgactacg agacccacct catcgagacc gccgacggcc
      541 aaaccgtcat cggcgtcatc cgcagccaca ccgccgaggg cctgctcgtc atggacgtgg
      601 ccggacagga gaaaaacctc ccccacgccg ccatcgccgc cagcacccag ctccccgcca
      661 gcctcatgcc catggggtta gatcaaaccc tgcccgagca ggacctcctc gacctcgtcg
      721 cctggctgcg cagcttgaag tgaccccaaa ccgatcaacg aatcgcagca tgatggcggg
      781 caggctgtag cgtgagcaac atggcacgaa gagccatcct actggggcgg ccggagctgg
      841 ccgccgagtc tgggtgccca agggcgcatg cccggtggtg gagcggcaga ccaggcatga
      901 atgagactac ctttacggct cgctgaaggt gaccggggga gaggcgcact ttgcccatct
      961 gcccaccgtg aacctcggct gcggggcgcg ggagtttacc ggcaaggatt ccgggggcgg
     1021 gcgcgtagtc tccggccatg tccactccca gcgaccgccg ttccttcctc aagcactccg
     1081 ctctcgccgc cgctgccgcg ggtttccccg ccgtggtgcg ctccgccagc ccgaactcga
     1141 tgctccaggt ggcgtccatc ggcgcggatg gcatggcgtt cagcgacatc aagaacatcg
     1201 gggcgcacgg gaaggtgcgc tatgtgggct tctgtgacat tgacagcacg cgcttcgcca
     1261 aggcggacgc ggcccacccg ggggtgccgc atttcgccga tttccgcgag atgctggcca
     1321 gcctgggcga caaggtggac gcggtgaacg tgggcacgcc cgaccacatg cacgcgaagg
     1381 cggccatcga ggccatgcgc cggggcaagc acgtctattg ccagaagccg ctggcgcaca
     1441 cggtgtggga gagccggcag atgcgcctgg aggcggaaaa ggccggcgtg gtcacccaga
     1501 tgggcaacca gatccactcc aacctggagt accgcctcgg cacgcggctg ctgaaggagg
     1561 gggccatcgg caaaatcaaa gaagtgtact cctgggtggg tgtgacgggc aacgagcgca
     1621 acaaaaagct ggagcccctg cccggcgccc ccgtgcccgc gcatgtgaac tgggacctgt
     1681 ggatcggcgt ggcgcccatg cgcgagtacg cgccctgcta ccatcctttc gtgtggcggg
     1741 actggcagga cttcggcggc ggcgcgctgg gggacttcgg ctgccacatt ctggaccccg
     1801 tcttcaccgc gctgggcctc aacgcccctc tgaccgtcac ggcgcgcaac agcggcatca
     1861 atgcccacat ctggccggtg aatgaggagg tggagtatgt cttcccaggc aacgcgctga
     1921 ccgcggacaa gaccctgaag gtgacgtgga gcgatggcgg cctgcgcccg gaccgcaaaa
     1981 aggcgaagat gccgccggac ctggagctgc cgcgcagcgg ctcgctcttc atcggcgagg
     2041 agggcaacat ggtgctggcc catgtggccg ggccgcggct ctacccgctg gagaagttcc
     2101 agggcttcgc ctacccgaag gaggagggca aaagccactg gcatgtctgg gtggacgcct
     2161 gcctcgcggg acaaaagacc agcgacggct tccactacgc cggcccgctc tccgaggccg
     2221 tgcagcttgg caatgtggcg acacgattct gcacgcgcgg ccccattgac tcccgcaccg
     2281 gcgaccctct gaaccccgtc atcctggaat gggacgcgga aaagctggcc ttcaccaacg
     2341 cccccgaggc caacccgctg ctgacgaaga cctaccgcga aggctgggcc atctgacccc
     2401 cggcccgcgc tgaggcgggc gccgtctcat ccgccaaaaa actccaccgc ctggcgcagg
     2461 atgccgagca tggcgtccac ctcctgctcc gtgttgtaga aataaaaact ggcgcggctg
     2521 gtgccgggga ctttgaagcg cctcatcagc ggctgagtgc agtggtggcc gccgcgcagg
     2581 gccaggccgc gggtgttcgc atactcgacg aggtcatgcg gatgcgcgca ttcgagtgcg
     2641 aaggcggcca gcggcgcgag cggctggccc gggccgagat cggaaga
//
LOCUS       DAOMYJ010000416         2685 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Prosthecobacter sp. isolate AMR_MDS_5629
            NODE_14256_length_2685_cov_15.748669, whole genome shotgun
            sequence.
ACCESSION   DAOMYJ010000416 DAOMYJ010000000
VERSION     DAOMYJ010000416.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867850
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prosthecobacter sp. (wastewater metagenome)
  ORGANISM  Prosthecobacter sp.
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Verrucomicrobiaceae; Prosthecobacter.
REFERENCE   1  (bases 1 to 2685)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2685)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.30
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:34:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,742
            CDSs (total)                      :: 4,684
            Genes (coding)                    :: 4,676
            CDSs (with protein)               :: 4,676
            Genes (RNA)                       :: 58
            tRNAs                             :: 55
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2685
                     /organism="Prosthecobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14256_length_2685_cov_15.748669"
                     /isolate="AMR_MDS_5629"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1965333"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..833)
                     /locus_tag="PLP58_22575"
     CDS             complement(<1..833)
                     /locus_tag="PLP58_22575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ11380.1"
                     /translation="MAKYHITYSCGHEDTVQLFGPCRDRERKIEWMEQHQLCPECYRA
                     SIDAKRAAASEAAARESEEIGLPDLEGSEKQVAWALRLRAEALKNLSENLKLKVPRFF
                     SKLELKHEMTDVLSAFEALALNWAAGKTSASWWIDSREAGWLDLFAKEERVSLERLQT
                     AVKDGLSLDVAAATVEAEKSADQMDERDIANAETMEPQERLSPLVAGVNIHSTKVTVR
                     FPEKNEALRTCVKQVGFRWAEVEWVKDCVAGEVDDAAVEVAWRLLKAGFRVRLAPEEV
                     R"
     gene            complement(837..1412)
                     /locus_tag="PLP58_22580"
     CDS             complement(837..1412)
                     /locus_tag="PLP58_22580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ11381.1"
                     /translation="MSNNQIEIHIAAAENKVTKVINSYGKKKTYHNGGFRVGVSIEGE
                     GDFICITKSATYSGLAESWGVLRAVEWAIAQGIKDLKVCTVDHCTSSRRKSGFFLKLA
                     HAKAEVAGMTLDVERIGKEDNDAVDLARDQDAKFLPRVADDTEEVKAAIAIVLKSYET
                     RDGEINPAAEEGMPELPVPESVEINPELVEG"
     gene            complement(1575..>2685)
                     /locus_tag="PLP58_22585"
     CDS             complement(1575..>2685)
                     /locus_tag="PLP58_22585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ11382.1"
                     /translation="ATRERAREIARKKVRELAAGAGARLVSAVEASALDRLRRMIGAR
                     ALSAAVEQLEDSVLRLGDWVHLGRAVDAYVRAGHGRVEARPLRAAVADFLAEHAARAP
                     MYVAGLRKELEAACATLGDVAVCDLSAEMLRAWMGRRLADGSAPGPRFGNNRLGTWKR
                     FLSWSRERGLLAGGEPHVLELVRPARLDDRAPPVWTPEQARAVLEVARAEMPEAVPYV
                     VVGCWLGCRPSEILRLKWADFDWSRGYLNVAADVARKVRRERFVPVPDNARAMLEPLA
                     SGPLHRVRGRRGRGHLCGLHVQRELMVRLRAAGVLEVWQQDVMRHSYISYRLAQGHGR
                     GQVAEWAGNSEGEILRSYRRPLRAEDGAAWFGVVV"
BASE COUNT          470 a         1040 c          673 g          502 t
ORIGIN      
        1 atccgcactt cttccggagc caagcgcacg cggaaccccg ccttgagcag acgccaggcc
       61 acttccactg cggcatcgtc cacctctccg gccacacaat ctttcaccca ctccacctcc
      121 gcccagcgga accccacctg tttcacgcat gtgcgcaacg cctcattttt ttccgggaat
      181 cggaccgtga cttttgtgga atgaatattc acgccagcaa ccaagggaga gagacgctct
      241 tgcggctcca tcgtttcagc attcgcaata tcccgctcat ccatctggtc cgccgatttt
      301 tcggcctcca ccgtggcagc cgccacatcc agtgacagcc cgtctttgac cgccgtctga
      361 aggcgctcca gagacaccct ctcctctttt gcaaacaggt ccagccagcc agcttccctg
      421 gaatcaatcc accagctcgc cgacgttttt cccgcagccc agttcaacgc cagagcctca
      481 aaagccgaga gcacatcagt catctcatgc ttcagctcca gctttgaaaa aaagcgcggc
      541 accttcagct tcaagttttc actcaaattc ttgagtgcct cagcacgcaa ccgcaatgcc
      601 caggccactt gcttttcgct gccctccaaa tccggcaggc caatctcttc ggactcccgc
      661 gcggcagcct cgctggctgc cgcgcgcttg gcgtcaatgg acgcccgata gcattcaggg
      721 cagagctgat gctgctccat ccactcgatt ttacgctcac gatcccggca gggtccgaaa
      781 agctgcaccg tgtcttcgtg cccgcaactg taggtgatgt ggtatttcgc catgactcag
      841 ccctccacaa gttcagggtt gatctcgaca gactcaggca ccggcagttc cggcatgcct
      901 tcctccgcag ccgggttgat ctcgccgtcg cgcgtctcgt agcttttcag cacgatggcg
      961 atggctgcct tcacttcctc ggtgtcgtcg gcgacgcggg gcagaaactt ggcgtcctga
     1021 tcgcgggcga gatcgacggc gtcgttgtct tccttgccga tgcgctcgac gtcgagcgtc
     1081 atgccggcga cttcggcctt ggcgtgagcg agcttgagga agaagccact cttgcgacgg
     1141 ctgctggtgc agtggtccac ggtgcagacc ttgaggtctt tgatgccctg ggcgatagcc
     1201 cattcaacgg cgcggaggac gccccaggac tccgccaggc cgctgtaggt ggccgatttc
     1261 gtgatgcaga tgaagtcgcc ctcaccctcg atgctcaccc cgactctgaa ccctccattg
     1321 tggtaggtct tctttttccc gtagctgttg atcaccttgg tgaccttgtt ttcagccgct
     1381 gcgatgtgga tttcaatctg gttgttgctc atgtcattta tcctctgtcg agttcgggga
     1441 gccgccccgg cgggtgaccg cagtttgcga tcacaagcgc actatgcgcc tttgttttgt
     1501 ggcgtcaaat caaaaaaatg attcaccttc aattttcagg ccttagcccc catttttcct
     1561 gcctcatccc ggcctcatac caccacccca aaccacgccg caccatcctc cgcgcgcaga
     1621 ggccggcggt agctgcgcag gatctccccc tcggaattcc ccgcccactc cgccacctgg
     1681 ccgcggccat gcccctgcgc caggcggtag ctgatgtagc tgtgccgcat cacatcctgc
     1741 tgccacacct ccagcacccc ggcggcccgc agtctaacca taagctctct ctgcacatgc
     1801 agtccgcaca gatgcccgcg cccccgcctg ccgcgcacac ggtgcagcgg ccccgacgcc
     1861 agcggctcca acatcgcccg cgcattgtcc ggcaccggca caaaccgctc ccggcgcacc
     1921 ttccgcgcca catcagccgc cacattgagg tagcctctgg accagtcaaa atccgcccac
     1981 ttcagccgca gaatctccga aggccggcag cccagccagc agccaaccac cacatacggc
     2041 accgcctccg gcatctccgc gcgcgccacc tccagcaccg cccgcgcctg ctccggcgtc
     2101 cacaccggcg gcgccctgtc atccagccgc gccggcctga ccagctccaa cacatgcggc
     2161 tccccgcccg ccagcagccc ccgctcgcgc gaccaggaca aaaagcgctt ccaggtgcct
     2221 aaccgattgt tcccaaatct cggccccggg gccgagccgt ccgccagtcg ccgccccatc
     2281 cacgcgcgga gcatctcggc ggagagatcg cacaccgcca catctcccag cgtcgcgcat
     2341 gccgcttcca gctccttgcg cagacccgcc acatacatcg gcgcgcgcgc cgcgtgctcg
     2401 gccaaaaaat ccgccaccgc cgcgcgcagc ggccgcgcct ccacccggcc atgcccggcc
     2461 ctcacatagg catccacagc ccggcccaaa tgcacccagt ctccgagacg cagcaccgag
     2521 tcctcgagct gctccaccgc tgcggagaga gcccgcgcgc cgatcatcct ccgcagcctg
     2581 tccaacgcag acgcctccac cgccgagacc aggcgcgcgc ccgctcccgc cgccagctcc
     2641 cgcacctttt tccgcgcgat ctcccgcgcc cgctcccgcg tcgcc
//
LOCUS       DAOMYJ010000417         2636 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Prosthecobacter sp. isolate AMR_MDS_5629
            NODE_14647_length_2636_cov_22.790391, whole genome shotgun
            sequence.
ACCESSION   DAOMYJ010000417 DAOMYJ010000000
VERSION     DAOMYJ010000417.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867850
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prosthecobacter sp. (wastewater metagenome)
  ORGANISM  Prosthecobacter sp.
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Verrucomicrobiaceae; Prosthecobacter.
REFERENCE   1  (bases 1 to 2636)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2636)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.30
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:34:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,742
            CDSs (total)                      :: 4,684
            Genes (coding)                    :: 4,676
            CDSs (with protein)               :: 4,676
            Genes (RNA)                       :: 58
            tRNAs                             :: 55
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2636
                     /organism="Prosthecobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14647_length_2636_cov_22.790391"
                     /isolate="AMR_MDS_5629"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1965333"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..979
                     /locus_tag="PLP58_22590"
     CDS             <1..979
                     /locus_tag="PLP58_22590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ11383.1"
                     /translation="KPKPPPLAAALLAFHCRLDTDTLEAELLVESFAEGWQLIPVAGP
                     GAGVVTFEPQDARLVFTEGQLCLLTDKPGRQTLRLSFAPPGADRPGALHLAPAAAASL
                     RVTGLPEGRSLHVRHGGGEFVAVQAGTVPLPAAGGEVLLSFQETGRPPQPATTGDAAL
                     ITRATYTTQVARDGSVLTEGVLETTRDQAAEARLQLPASARLLRCRVGDSTLRLTPDG
                     GNQITVPLPAPGADKAPDGVIHLSYTDRLPTLDPAEGELELALPGTPLFARQIEWNVL
                     LPAAYELAFSGSMDAADDPPKTPGLHLRQSFCRDQQPQVRVTYRKRTSL"
     gene            1020..2204
                     /locus_tag="PLP58_22595"
     CDS             1020..2204
                     /locus_tag="PLP58_22595"
                     /inference="COORDINATES: protein motif:HMM:NF017893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane CreD family protein"
                     /protein_id="HRJ11384.1"
                     /translation="MKLTHLFTIAFIFVCTTFAWWLLGSTITYRTHHVAAQTGDSVSG
                     RWGPPLVQTHPRARYTSSNGTSVTLQPARSEVRADLRYQPVKMGLLWHRTYGVKFAGD
                     YTFTNNTSITQTLSIDFELPPGNTLLDKIVFIIGEGGKARRSVAAPEQGVITDSVQLA
                     PGESIPVTVGYECRGMDTWRYIFPGAGRIRDFTLTMSTNFDDVNFPVSSPTDRESAAD
                     GMTLVWKYEDAISAPGIAMDMPRELNAGPVAAQISMYAPLSLLLFFSVLLITCVTRGI
                     ALHPVNYFFIAAGFFTFPLLFAYMLDLAPVHLSFIVAAGASLALVCGYLRAAGGLFLF
                     RVALAAQTAYMVLFSYSFFFKGLTGLTLTLGGVATLGMLMALTAKVDWSSKLGAVSPK
                     LA"
     gene            2244..>2636
                     /locus_tag="PLP58_22600"
     CDS             2244..>2636
                     /locus_tag="PLP58_22600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009965609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HRJ11385.1"
                     /translation="MSPNSAAMPARILVVEDEPSICENIVYALEADGFAVETAGTGFQ
                     ALEALRAGAFQLVILDVGLPDMTGFEVCKTLRQSSQTPVIFLTARSSEVDRVVGLEIG
                     GDDYVVKPFSPRELAARARAILRRARPDA"
BASE COUNT          405 a          954 c          812 g          465 t
ORIGIN      
        1 gaagcccaag ccgccgccgc tggccgccgc cctgctcgcc ttccactgcc gtctcgacac
       61 ggacacgctg gaggccgagc tgctcgtgga gagttttgcg gaagggtggc agttgatccc
      121 cgtggcgggc cccggcgcgg gtgtcgtgac cttcgagccg caggacgcgc ggctggtctt
      181 caccgagggc cagctttgcc tgctcaccga caaaccgggg cggcagacat tgaggttgtc
      241 ctttgcgccg cccggagctg accggcccgg cgcgctgcat ctggcaccgg cggcggccgc
      301 ttcactgcgg gtcaccgggc tgccggaggg caggagcctg catgtgcgcc atggcggggg
      361 tgaatttgtg gcggtccagg ccggcaccgt gcctctgccg gccgcgggcg gcgaggtgct
      421 gctctctttt caggagacgg gcaggcctcc ccagcccgcc accaccgggg atgcggccct
      481 catcacgcgc gccacctaca ccacccaggt ggcgcgggac ggctccgtgc tcaccgaggg
      541 tgtgctggaa accacccgcg accaggctgc cgaggcgcgc ctccaattgc ccgcgtcggc
      601 ccggctgctg cgctgccgtg tgggtgacag caccctgcgt ctcacgcccg acggtggaaa
      661 tcaaatcacc gtgccgctgc cagctccggg cgcggacaag gcgccggacg gcgtcattca
      721 cctttcctac acagatcgtc ttccgaccct ggaccccgcc gagggcgagc tggagctggc
      781 cctgcctggc accccgctct ttgcccggca gatcgagtgg aatgttctcc tgcccgccgc
      841 ctacgagctc gctttcagcg gcagcatgga cgccgcggac gatccgccaa agacgcccgg
      901 cctccatctc cgccagtcct tctgccgcga ccagcagccg caagtccgcg tcacctaccg
      961 caagcgcacc tccctctgaa ctccgaaccc gaatccaaac ctgaatctca cgacatccca
     1021 tgaaactgac ccacctcttc accatcgcct tcatctttgt ctgcaccacc ttcgcgtggt
     1081 ggctcctcgg cagcaccatc acctatcgca cgcatcacgt cgccgcccag acaggggaca
     1141 gcgtcagtgg gcgctggggt ccgccgctgg tacagaccca tcccagggcg cgctacacct
     1201 cctcaaatgg cacgagtgtc accctccagc ccgcgcgcag cgaggtgcgg gcggaccttc
     1261 gttatcaacc ggtcaaaatg ggactgctct ggcaccgcac ttacggagtg aagtttgctg
     1321 gcgactacac cttcacgaac aacacctcca tcacccagac gctgtccatt gacttcgagc
     1381 tgccgcccgg caacacgctg ctcgacaaga tcgtcttcat catcggcgaa ggcggaaaag
     1441 cgcgccgctc cgtggccgcg cccgagcagg gcgtcatcac ggacagcgtc cagctcgcac
     1501 ccggcgagag catcccggtg acggtcggct acgaatgtcg cggcatggac acctggcgct
     1561 atatttttcc cggagcgggc cgcatccgcg acttcacgct gacgatgagc acgaactttg
     1621 acgacgtgaa cttccccgtc agctccccca ccgatcgcga gtccgcggcg gatggcatga
     1681 cgctggtctg gaaatacgag gacgccatct ccgcgccggg aatcgccatg gacatgccgc
     1741 gcgagctgaa cgccggcccg gtggcggcgc agatttccat gtatgcgccg ctttccctgc
     1801 tgctgttctt cagcgtcctg ctcatcacct gcgtgacacg cggcatcgcg ctgcatccgg
     1861 tgaactattt cttcatcgcc gccggcttct tcacctttcc gctgctgttc gcctacatgc
     1921 tcgacctcgc gccggtgcat ctgagcttca tcgtcgccgc gggcgcctcg ctggcgctgg
     1981 tctgcggcta tctgcgcgcc gccggcggcc tgtttctttt ccgcgtggcc ctggcggcgc
     2041 agacggccta catggtgctc ttcagctaca gcttcttttt caaggggctc accgggctca
     2101 ccctcactct cggcggcgtg gccacgctcg gcatgctgat ggcgctgacc gcgaaggtgg
     2161 actggagcag caagctgggc gccgtgtcac cgaagctggc atgagaaaat gccgtttgca
     2221 gtgtgccgcg cccacgcgcc accatgagcc cgaactccgc cgccatgccc gcccgcattc
     2281 ttgtcgtcga ggacgagccc tccatctgcg aaaacatcgt ctatgccctc gaggcggacg
     2341 gcttcgcggt ggagacggcg ggcacgggct tccaggcgct tgaggcgctg cgggcgggcg
     2401 ccttccagct tgtgatcctt gatgtgggcc tgcctgacat gaccggcttc gaggtctgta
     2461 aaacgctgcg ccagtccagc cagacacccg tgatcttcct caccgcgcgc tcctccgaag
     2521 tggatcgtgt ggtggggctg gaaatcggcg gtgacgacta tgtggtgaag cccttcagcc
     2581 cgcgcgagct ggccgcccgc gcgcgcgcca tcctgcgccg ggcgcggcct gacgcg
//
LOCUS       DAOMYJ010000418         2625 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Prosthecobacter sp. isolate AMR_MDS_5629
            NODE_14730_length_2625_cov_16.202724, whole genome shotgun
            sequence.
ACCESSION   DAOMYJ010000418 DAOMYJ010000000
VERSION     DAOMYJ010000418.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867850
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prosthecobacter sp. (wastewater metagenome)
  ORGANISM  Prosthecobacter sp.
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Verrucomicrobiaceae; Prosthecobacter.
REFERENCE   1  (bases 1 to 2625)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2625)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.30
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:34:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,742
            CDSs (total)                      :: 4,684
            Genes (coding)                    :: 4,676
            CDSs (with protein)               :: 4,676
            Genes (RNA)                       :: 58
            tRNAs                             :: 55
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2625
                     /organism="Prosthecobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14730_length_2625_cov_16.202724"
                     /isolate="AMR_MDS_5629"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1965333"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..191
                     /locus_tag="PLP58_22605"
     CDS             <1..191
                     /locus_tag="PLP58_22605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ11386.1"
                     /translation="AARLTGGAVLKPGELPALDATRAVQYQELRPALLLALLALLLAD
                     VLIRRWEHVQAFIPRLQK"
     gene            complement(234..2561)
                     /locus_tag="PLP58_22610"
     CDS             complement(234..2561)
                     /locus_tag="PLP58_22610"
                     /inference="COORDINATES: protein motif:HMM:NF019259.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1592 domain-containing protein"
                     /protein_id="HRJ11387.1"
                     /translation="MAATSTRFLSSWLILWSASAAFAAEAAHPGKAIYDKQCLECHGD
                     RGQGVEGRYDEPLIGERTLEALARKIEKTMPEDNEDACVGEDAKAVAEYIYHTFYSPE
                     AQARVNPPARDLARLTAPQYRNSVADLIGRFRMGAGFDRPLGGEPGLKAIYRGVELPK
                     PGEKAVDTTPKDGIKKKRPNKTIERVEPVISFHWGEGSTVPETLEAEQFESRFEGCVI
                     PRETGVHEFAVKSENGFRLWINDDDEADALIDGWVSAGPKVREEKKSIFLVGGRAYQL
                     LLEHFKFKEASSSLELWWRPPHGVWELLPARATRSTRPRELMIVDTSFPADDRSAGYE
                     RGTTISKEWQQAVTEAAVATAEHVVEYLDELAQTKPGAPDRPEKLRQFARVFVETAFR
                     RPLTDEQKNLHIEAQFQTAPTPETAVKRVVLLALKSPQFIYPGIGAGEKPDDFETASR
                     LALTLWDSLPDKRLWQAAAEGRLRRPEQVRAEARRMVADARTRAKLHGFFHHWLDLEH
                     AESTSKDPKAFPGFDQAVLADLRESLLRFVDDVVWSEASDYRELLQADYLLLNERLGK
                     FYGKPVQGGEFQKVSFDPRQRAGVVTHPYLLASLSYARQTSPIHRGVFLTRNIVGMSL
                     KPPQVAVSFDESRFDPGLSMREKITELTKGGACMSCHSVINPLGFSLENFDAIGRWRT
                     RDNNKPVNPVSDFSDELGNTVHLSGPRDIVNHVADNPAGHRAFIRHLFHHLVKQQVPA
                     YGPNVLDELQRKFAAEGCGIRQLVEEIALVAATAP"
BASE COUNT          405 a          835 c          889 g          496 t
ORIGIN      
        1 aggccgcgcg cctcaccggc ggcgccgtgc tcaagccggg cgaactgccc gcgctcgacg
       61 ccacccgcgc ggtgcaatac caggagctgc ggcccgcgct gctgctggcg ctgctggcgc
      121 tgctgctggc ggatgtgctc atccgccgct gggagcatgt gcaggcgttt attccgcgcc
      181 tgcaaaaatg acgcggatgc ttcaagaatg cccgcgccac ggctggaagg cttttacgga
      241 gccgtggcgg cgacgagggc gatctcctcg acgagctggc ggatgccgca gccttcggcg
      301 gcgaatttcc gctgcaattc gtcgagcacg ttcggtccat aggcggggac ctgctgcttg
      361 acgaggtggt ggaacagatg acggatgaag gcgcgatgac cggcggggtt gtcggcgacg
      421 tggttgacga tgtcccgcgg tccgctgaga tggacggtgt ttcccagctc gtcgctgaag
      481 tcacttacgg ggttgacggg cttgttgttg tccctggtgc gccagcgtcc gatggcgtcg
      541 aagttctcga ggctgaagcc gagggggttg atgacactgt ggcaggacat gcaggcgccg
      601 cccttggtga gctcggtgat cttttccctc atgctcaggc cgggatcaaa acggctctcg
      661 tcgaagctca cggccacctg gggcggcttc aatgacatgc cgacgatgtt gcgggtgagg
      721 aagacgccgc gatggatggg agaggtctgc cgcgcgtagg agagggaggc gagcaggtag
      781 ggatgagtga ccacgccggc gcgctgtctg gggtcgaagg agactttctg gaattcaccg
      841 ccctggacgg gcttgccgta gaatttgccg agcctctcgt tgagcagcag gtagtcggcc
      901 tggagcagct cgcggtagtc ggaggcctcg ctccacacca catcatccac aaagcgcagc
      961 agggactcgc gcaggtcggc cagcacagcc tggtcgaaac cggggaaggc cttcggatcc
     1021 ttcgaggtgc tctcggcgtg ctccaggtcc agccagtggt ggaagaagcc gtgcagcttg
     1081 gcccgcgtgc gcgcgtccgc caccatgcgc cgcgcctcgg cgcggacctg ctcgggtctg
     1141 cgcagcctcc cctcggcggc tgcctgccag aggcgcttgt ccgggaggga atcccacaac
     1201 gtcaatgcca ggcgggaggc ggtctcaaag tcgtcgggct tttccccggc gccgatcccc
     1261 ggatagatga actgcggcga cttcagggca agaagcacca cgcgcttcac ggcggtctcc
     1321 ggcgtcggcg cggtctggaa ctgagcctcg atgtggagat tcttttgctc atcggtgagc
     1381 ggacgccgga aggcggtctc gacaaagacg cgggcgaatt ggcgcagctt ttccggacgg
     1441 tcgggcgcgc cgggtttggt ctgcgccagc tcgtcgagat actccaccac atgctcggcg
     1501 gtggcgacgg cggcctcggt gaccgcctgc tgccattctt tggaaatggt cgtgccgcgc
     1561 tcgtagcccg cgctgcggtc gtcggcgggg aagctggtgt caacgatcat cagctcgcgc
     1621 gggcgcgtgc tgcgcgtggc acgcgcgggc agcagctccc acacgccgtg cggcggccgc
     1681 caccacagct caagggagga ggatgcctcc ttgaatttga aatgctccag caggagttgg
     1741 taggcgcgcc ctccgacgag gaagatgctt tttttctcct cgcgcacctt cggccctgcg
     1801 cttacccagc cgtcaatgag cgcgtccgcc tcgtcgtcgt cattgatcca gagacggaag
     1861 ccgttttccg acttcaccgc aaactcatgc acccctgtct cgcgcggaat gacgcagcct
     1921 tcaaagcggc tctcgaactg ctccgcctcc agcgtctcgg gcacggtgct gccctcgccc
     1981 cagtgaaagg agatcaccgg ctccacgcgc tcgatggttt tgttgggccg ctttttcttg
     2041 atgccgtcct tcggtgtcgt gtccaccgcc ttttcccccg gcttcggcag ctccacgccg
     2101 cggtagatgg ccttcaaccc aggctcgccc ccgagcgggc ggtcgaagcc cgcgcccatg
     2161 cggaagcgcc cgatgaggtc ggccacgctg ttgcggtatt gcggcgctgt gaggcgcgcc
     2221 agatcccgcg cgggcgggtt gacgcgcgcc tgcgcctccg gcgagtaaaa ggtgtggtag
     2281 atgtactcgg ccaccgcttt cgcgtcttcg cccacgcagg cgtcctcgtt gtcctccggc
     2341 atcgtctttt cgatcttgcg cgccagtgcc tcaagcgttc gctcgccgat gagcggctcg
     2401 tcataccttc cctccacgcc ctggccgcgg tcgccgtggc attcgaggca ttgtttgtca
     2461 tagatggcct tcccgggatg cgccgcctcc gccgcgaaag cggctgacgc gctccagaga
     2521 atgagccagg atgaaaggaa acgggtggat gtggcagcca tgactttttg agggggcgga
     2581 gaaccgtgcc tggaggatta aatccccagc ccgccacctc tttca
//
LOCUS       DAOMYJ010000419         2603 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Prosthecobacter sp. isolate AMR_MDS_5629
            NODE_14906_length_2603_cov_20.946232, whole genome shotgun
            sequence.
ACCESSION   DAOMYJ010000419 DAOMYJ010000000
VERSION     DAOMYJ010000419.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867850
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prosthecobacter sp. (wastewater metagenome)
  ORGANISM  Prosthecobacter sp.
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Verrucomicrobiaceae; Prosthecobacter.
REFERENCE   1  (bases 1 to 2603)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2603)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.30
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:34:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,742
            CDSs (total)                      :: 4,684
            Genes (coding)                    :: 4,676
            CDSs (with protein)               :: 4,676
            Genes (RNA)                       :: 58
            tRNAs                             :: 55
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2603
                     /organism="Prosthecobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14906_length_2603_cov_20.946232"
                     /isolate="AMR_MDS_5629"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1965333"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2603)
                     /locus_tag="PLP58_22615"
     CDS             complement(<1..>2603)
                     /locus_tag="PLP58_22615"
                     /inference="COORDINATES: protein motif:HMM:NF012311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HRJ11388.1"
                     /translation="QMMAAARPRAEDRFAGLPVLEEKLHEARAAGESVTRRLRLVRDE
                     SFKHPLIRVEDEMSGGRLLRQRAMVADHVMVKPLDPRMPEADLLKRLDVPGASVRRRM
                     PASGVWLVAFPLAGLETVPDALARFARLRDVVRVAEPDYIVHAGTTAYPNDPSFNELW
                     GMDNTGQSGGVADADIDAPEAWSLHTGSSSVVVAVIDSGMDMSHPDLHPNLWTNPGEI
                     PGNGIDDDGNGYVDDAHGWNFVNNTYNPQDDHGHGTHCAGTIGAAGDNSEGVAGVCWE
                     VSLMPLKFLDSSGNGTLADAVEAVAYSTMMGVTLSSNSWGGGGFSQALLDEIVAADSA
                     GILFVAAAGNAALNMDVTPDYPGSYNVPNLITVASTTSADGLSSFSNFGLTTTHLGAP
                     GSDIYSTLPGGGYGFNSGTSMAAPHVSGACALLKSFKPGLTHHQMRALVLGTVDAIPA
                     LQGRTVTGGRLNAFSALMASDDILLSPSQELTVRGPVGGPFTPGAQVFTLSNQSPDTA
                     PWSFSVNRSWVTLSAAEGVLPPGASTTVTATLNEEAGGLLAGVRTATFTLHNSGTGRS
                     QTRIMRVRVTPPPLYSFDLDTDPGWARDGQWQHGAPQGQGALNFGFPDPASGATGSSV
                     FGVNLAGDYTADPGSAQYLTAGPFDLTGMNSVSVRFKRWLNTDHLPWVEAAVEVSNDG
                     VFWAALWDNGGAAITDSNWTEVEQDLSLIADGRPQVHVRWKHHVTASGAYPYSGWNLD
                     DIEILGVPAVQLRMTLPASVEEGGSPVLAKITALPVLPSDLVVSLSSSRPGEKLGLPA
                     TVVIPAGEEEAEFVITPLQDTLVDGSQAVLVTASAAGYPSHEAQVLVHDDEQGALGLI
                     LPATAAEGAGV"
BASE COUNT          418 a          937 c          859 g          389 t
ORIGIN      
        1 gcacacccgc gccctccgcc gccgtggccg gcaggatgag gcccagcgcg ccctgctcgt
       61 catcatgcac gagcacctgc gcctcatgcg acgggtagcc cgccgcgctg gcggtgacga
      121 gcacggcctg ggagccgtcc accagcgtgt cctggagcgg cgtgatgaca aactccgcct
      181 cctcctcgcc agccgggatg accaccgtgg cgggcaggcc cagcttttcc cccggcctgc
      241 tggagctgag gctgacgacc agatcggacg gcagcaccgg cagcgcggtg atcttcgcca
      301 gcactggaga gccgccttcc tcgacggacg ccggcagtgt catgcgcaac tgcacggcgg
      361 gcacgccgag gatctcgatg tcgtccagat tccagccgga gtaaggatac gcgccgctgg
      421 ctgtgacgtg gtgcttccag cgcacatgca cctgcggccg gccgtcggcg atgagggaga
      481 ggtcctgctc cacctccgtc cagttggagt cggtgatggc cgcgccgccg ttgtcccaga
      541 gcgcggccca gaaaacgccg tcgttcgaga cctcgacggc cgcctccacc cagggcaggt
      601 ggtccgtgtt caaccagcgc ttgaagcgca ccgacacgct gttcatgccg gtcaggtcaa
      661 acggccccgc cgtcagatat tgagcggagc cgggatcggc agtgtagtcg ccggccaggt
      721 tgacgccgaa gacggaggag cccgtggcgc cggaagcagg gtcgggaaag ccgaagttca
      781 aagcgccctg gccctgcggc gcgccgtgct gccattgtcc gtcccgcgcc cagccggggt
      841 cggtgtcgag atcgaaggag taaagcggcg gcggcgtcac ccgcacgcgc atgatccggg
      901 tctgggagcg gcccgtgccg gagttgtgca aggtgaaggt ggccgtcctc actcccgcga
      961 gcagaccgcc ggcctcctcg ttgagcgtgg ccgtgaccgt ggtgctggcg cccggcggca
     1021 gcacgccctc cgcggcggaa agcgtgaccc aggagcggtt cacggaaaag ctccacggcg
     1081 cggtgtcggg gctttgattg gaaagcgtga acacctgcgc tcccggcgtg aacggcccgc
     1141 ccaccggacc gcgcaccgtc agctcctggg agggggagag caggatgtca tcgctggcca
     1201 tcagcgcgct gaaagcgttc agccggccgc cggtgacggt gcggccttgc agcgcgggga
     1261 tcgcatccac cgtgccgagc accagcgcgc gcatctggtg gtgggtgagg ccgggcttga
     1321 aggacttcaa aagcgcgcac gcgcccgaga catgcggcgc ggccatcgaa gtcccgctgt
     1381 tgaagccgta gccgccgccg ggcagcgtgc tgtaaatgtc gctgccgggc gcgccgagat
     1441 gcgtcgtcgt caggccgaag ttcgagaagg aggaaagccc gtccgccgag gtggtggagg
     1501 ccacggtgat caggttcggc acattgtagc tgccgggata gtcgggcgtc acgtccatgt
     1561 tcagcgccgc attgcccgcc gccgccacaa agaggatgcc cgcgctgtcc gccgccacga
     1621 tctcatccag caaagcctgc gagaacccgc cgccgcccca cgagttcgag gaaagcgtca
     1681 ctcccatcat cgtcgagtag gccaccgcct cgaccgcgtc cgccagcgtg ccgttgccgg
     1741 acgaatcgag aaatttcagc ggcatgagcg agacctccca gcacacgccg gccacgccct
     1801 cggagttgtc ccccgccgcg ccgatggtgc ccgcgcagtg cgtgccgtgg ccgtggtcat
     1861 cctgggggtt gtaggtgtta ttgacgaagt tccacccgtg cgcatcgtcc acatagccgt
     1921 tgccatcatc atcaatgccg ttgccgggga tttctcccgg attggtccac aggttcgggt
     1981 ggaggtccgg gtggctcatg tccatgcccg agtcaatgac cgccaccacc accgaacttg
     2041 accccgtgtg cagcgaccac gcctccggcg cgtcaatgtc cgcgtccgcc accccgccgc
     2101 tctggccggt gttgtccatg ccccacagct cgttgaagga cgggtcgttc gggtaagccg
     2161 tcgtgcccgc gtggacgatg tagtcaggct ccgccacacg caccacatcc cgcagccgcg
     2221 cgaaccgcgc cagcgcgtcc ggcaccgtct ccaggcccgc cagcggaaaa gccaccagcc
     2281 acacgccgga ggccggcatc ctccgccgca cgctggcgcc gggcacatcc agccgcttga
     2341 gcaaatccgc ctccggcatc cgcgggtcca gcggcttcac catcacatga tccgccacca
     2401 tcgcccgctg ccgcagcagc ctgccgccgg acatctcgtc ctccacgcgg atcagcggat
     2461 gtttgaacga ctcgtcgcgc accagccgca gccgccgcgt cacactttcc ccggcggcac
     2521 gcgcctcgtg cagcttttcc tccagcaccg gcagcccggc aaagcggtcc tccgcccgcg
     2581 ggcgcgccgc cgccatcatc tgc
//
LOCUS       DAOMYJ010000420         2596 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Prosthecobacter sp. isolate AMR_MDS_5629
            NODE_14958_length_2596_cov_20.624951, whole genome shotgun
            sequence.
ACCESSION   DAOMYJ010000420 DAOMYJ010000000
VERSION     DAOMYJ010000420.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867850
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prosthecobacter sp. (wastewater metagenome)
  ORGANISM  Prosthecobacter sp.
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Verrucomicrobiaceae; Prosthecobacter.
REFERENCE   1  (bases 1 to 2596)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2596)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.30
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:34:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,742
            CDSs (total)                      :: 4,684
            Genes (coding)                    :: 4,676
            CDSs (with protein)               :: 4,676
            Genes (RNA)                       :: 58
            tRNAs                             :: 55
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2596
                     /organism="Prosthecobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14958_length_2596_cov_20.624951"
                     /isolate="AMR_MDS_5629"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1965333"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1946)
                     /locus_tag="PLP58_22620"
     CDS             complement(<1..1946)
                     /locus_tag="PLP58_22620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009961489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase family protein"
                     /protein_id="HRJ11389.1"
                     /translation="MAIHVALRHVTRYDFDRPVSLSPHVVRLRPAPHCRTKVLSYSLK
                     VTPEQHFINWQQDPQSNHLARLVFPEKTRSLCVEVDLVAEMAVYNPFDFFLEPQAEHF
                     PFKYDPALAHELEPFHRKLPLTPQFTAYLREVRHKLLNGRGPLPHAPVPHSETADTQP
                     HGILPEPPHEPVPDTDKRPRSMDFLVQINQMLWSDIRYTIRMEPGVQTPEETLELLSG
                     SCRDSAWLLVQLLRHLGLAARFVSGYLIQLKPDVKALDGPSGAETDFTDLHAWCEVYL
                     PGAGWIGLDPTSGLLAGEGHLPLAATPDPSSAAPITGLVDKCEVQFGHEMHISRVHET
                     PRVTKPYTPEQWAAIEALGHRIDSDLLSHDVRLTMGGEPTFVSIDDMDGEEWNTAAVG
                     PQKRLLSGQLIKRLRARFAPGALLHYGTGKWYPGEPLPRWALGCYWRKDGVPVWKDDS
                     LIADESKNLGHNEADARAFGLALASALQVDTRWLKEAYEDVYYYLWREKRLPVNVDPL
                     QSNLKDELERARLARLFEHGLDQITGFILPIERAWQGGQLVWKSGPWFVRDDTLHLIP
                     GDSPMGLRLPLDSLPWVSAGDYPWIYPTDPASDWPELPPRPDSRQRIVEQIAGSLYGP
                     GATSTPPASWRQGIHAGQGKPERR"
     gene            complement(2215..>2596)
                     /locus_tag="PLP58_22625"
     CDS             complement(2215..>2596)
                     /locus_tag="PLP58_22625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ11390.1"
                     /translation="GGMKRALLSALLSTAAVHAETAGHWDLDALKQPPVMRWEDETGP
                     VRSLIYEGAPDAKGARTEVFAFYASPATDGGKAKPPFPGVILIHGGGGTAFADWVHLW
                     ARRGYAALAMNLNGSRPPAPAFGS"
BASE COUNT          411 a          691 c         1052 g          442 t
ORIGIN      
        1 ttccggcgct cgggcttgcc ctggccggcg tggatgccct gacgccagga ggcgggcggg
       61 gtgctggtgg cgccggggcc gtaaaggctg ccggcgatct gctcgacgat gcgctggcgg
      121 ctatcggggc ggggcggcag ttcgggccag tcgctggcgg ggtcggtggg gtagatccag
      181 gggtagtcac cggcgctgac ccaggggagg ctgtcgagcg gcaggcgcag gcccatgggg
      241 gagtctcccg ggatgaggtg gagggtgtcg tcacggacga accaggggcc gcttttccag
      301 acgagctggc cgccctgcca ggcgcgctcg atggggagga tgaagccggt gatctggtcg
      361 agcccgtgct cgaagaggcg ggccaggcgg gcgcgctcca gctcgtcttt gaggttggac
      421 tggagggggt ccacattgac ggggaggcgt ttttcccgcc agaggtagta gtacacgtcc
      481 tcataggctt ctttgagcca gcgggtgtcc acctggaggg cggaggcgag ggcgaggcca
      541 aaggcgcggg cgtcggcttc gttgtggccg aggtttttgg attcgtcggc aatgaggctg
      601 tcgtccttcc agacggggac gccgtccttg cgccagtagc agccgagagc ccagcgggga
      661 agtggctcgc cgggatacca tttgccggtg ccatagtgga ggagcgcacc gggggcgaag
      721 cgggcgcgca ggcgcttgat gagctggccg gagagcagtc gcttttgcgg gccgacggcg
      781 gcggtgttcc actcctcgcc gtccatgtcg tcaatgctga cgaaggtggg ctcgccaccc
      841 atggtgaggc gcacgtcatg ggaaaggagg tcgctgtcaa ttcggtggcc gagggcctcg
      901 atggcggccc attgctcggg ggtgtagggc ttggtgacgc ggggcgtctc atgcacgcgg
      961 ctgatgtgca tttcatggcc gaactggacc tcgcatttgt ccacgaggcc ggtgatgggg
     1021 gcggcgctgg aggggtccgg cgtggcggcg agggggaggt ggccttcccc ggcaaggagg
     1081 ccggaggtgg ggtcgaggcc gatccagcct gcgccgggga gatagacctc gcaccaggcg
     1141 tggaggtcgg tgaagtcggt ctcggctccg ctggggccgt cgagggcttt gacgtcgggc
     1201 ttgagctgga tgaggtagcc gctgacgaag cgggcggcga ggccgaggtg gcggaggagc
     1261 tggacaagaa gccaggcgga gtcgcggcag gagccgctga ggagttcgag ggtctcctcg
     1321 ggcgtctgca cgccgggttc catgcggatg gtgtagcgga tgtcgctcca gagcatttgg
     1381 ttgatctgga cgaggaagtc catgctgcgc gggcgtttgt ccgtatctgg gacgggctcg
     1441 tggggaggct cggggaggat gccgtggggc tgggtgtcgg cggtctcgga gtgggggacc
     1501 ggagcgtgcg gcagtgggcc gcgaccgttg agcagcttgt gccgcacctc gcgcagatag
     1561 gcggtgaact gcggggtgag aggcagcttt cgatggaaag gctccagttc gtgggccagg
     1621 gcgggatcgt atttgaaggg aaagtgctcg gcctgcggct ccaggaagaa atcaaaggga
     1681 ttatagacgg ccatctcggc cacgaggtcc acctcgacgc aaaggctgcg ggttttctcg
     1741 gggaagacga ggcgggcgag gtggttggac tgcgggtcct gctgccagtt gatgaaatgc
     1801 tgctccggcg tgaccttcaa cgagtagctg agcaccttgg ttcggcaatg cggcgccggg
     1861 cgcaggcgca cgacgtgcgg ggaaagcgag acgggacggt caaagtcgta ccgtgtgacg
     1921 tgacgaaggg cgacgtgaat ggccatgcag ggggggttgg tattctcatg ccaaagcacg
     1981 gcgcgtgcca gcgggaggct gggaaagtta aagtccgaac ttcgaagctc gatattcgaa
     2041 gttctgaggg ggggattccg acttgccatt tagagcctgt ccggcaaata gctggaacga
     2101 gtaaacccgg gtgccatggt ggcgatgcat gtccagcaag acgtgtcaac ccgccggcca
     2161 cgccaccgac ctcacagacg ctcaatgggc gttgattgaa ccctcctctt tccctcagga
     2221 gccaaacgcg ggcgcgggcg ggcgggagcc gttcaggttc atggccaggg cggcatagcc
     2281 gcgccgcgcc cagaggtgga cccagtcggc aaaggcggtg ccgcccccgc cgtggatgag
     2341 gatgacgccg ggaaagggcg gcttggcttt tccgccgtcg gtggcggggc tggcatagaa
     2401 ggcgaagacc tcggtgcgcg cgcctttcgc gtccggcgcg ccttcgtaga tgagggagcg
     2461 cacggggccg gtctcatcct cccagcgcat gacgggcggc tgcttgaggg cgtcgaggtc
     2521 ccagtggccg gcggtttcag catggacggc ggcggtgctg agcagggcgg aaaggagggc
     2581 gcgcttcatg ccgcca
//
LOCUS       DAOMYJ010000421         2580 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Prosthecobacter sp. isolate AMR_MDS_5629
            NODE_15117_length_2580_cov_20.064950, whole genome shotgun
            sequence.
ACCESSION   DAOMYJ010000421 DAOMYJ010000000
VERSION     DAOMYJ010000421.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867850
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prosthecobacter sp. (wastewater metagenome)
  ORGANISM  Prosthecobacter sp.
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Verrucomicrobiaceae; Prosthecobacter.
REFERENCE   1  (bases 1 to 2580)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2580)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.30
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:34:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,742
            CDSs (total)                      :: 4,684
            Genes (coding)                    :: 4,676
            CDSs (with protein)               :: 4,676
            Genes (RNA)                       :: 58
            tRNAs                             :: 55
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2580
                     /organism="Prosthecobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15117_length_2580_cov_20.064950"
                     /isolate="AMR_MDS_5629"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1965333"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1244
                     /locus_tag="PLP58_22630"
     CDS             <1..1244
                     /locus_tag="PLP58_22630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ11391.1"
                     /translation="AASAEAVAGMFFGGGLLLLMAGLSLARQWLRRPRGLADSLAQIG
                     LRNLARRPSRSLAVMGMMAAGIFLVTAVNAFRMSAGDAARKDSGTGGFTLVGESSLPV
                     YEDLNTQAGRDAFGLDEEIMEGVRVAPFRVRQGDDASCLNLNRAQKPVLAGVNPALLA
                     GRFRFASGNWEALAEPQAAIADQATAMWGLGIGMGDALEYQDGAGRAWSVRLAGMLAG
                     SVLQGKMILGEDAFLKAYPDTAGYRFFLIETAPERAGEVAAHLTRQLEKRGLALEPAR
                     QRLETYLSVQNTYIGIFTVLGGLGVLLGTAGLGILVSRHVLERKGELGLMQAVGFLPG
                     ALRRMVLGEHVFLLLAGLLLGVVSALLAVWPALRQGGGDLPLAFLGLLLAGVLAFGIL
                     ACALAVQAAVRGRLIEAVRRE"
     gene            1341..2435
                     /gene="hisC"
                     /locus_tag="PLP58_22635"
     CDS             1341..2435
                     /gene="hisC"
                     /locus_tag="PLP58_22635"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009962446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004400 - histidinol-phosphate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HRJ11392.1"
                     /translation="MSIWNHANPQLKDLVAYEPGKPIEDVARELGLRPEDIIKLASNE
                     NPLGPSPKAVEAMKQAVEEVHIYPDGASWKLRNALAEKFGLEMGNFIMGCGSNEVIEF
                     IGHAFLKPGDNIITAEHAFLVYKLMAKVFGADTIEVPDPGYVHDLDAMAAAVTPRTKE
                     VFIANPNNPTGTLVTQEQIDRFMDKVPEHVVVVFDEAYYEFLDNPPDTLKYVREGRKV
                     VVLRTFSKIQGLAGTRVGYGIANKELIDVLQRTRQPFNLNSIAQAGALAGLLDEEHQA
                     KTKRITDEGRAYLQKQFGAMGLEYIPSHANFVLVKVGDGNAVFKAMMAKGVIVRAMAA
                     YKLPEWVRISIGTMPQNERCIEVLKGVLGR"
BASE COUNT          420 a          819 c          901 g          440 t
ORIGIN      
        1 ccgccgcgtc ggcggaggcg gtggcgggca tgtttttcgg cggcggtttg cttttgctga
       61 tggccgggct gtccctggcg cgccaatggc tgcggcgccc gcgcgggctg gcggactcgc
      121 tggcgcagat cggcctgcgg aacctggcgc gccggccttc gcgcagcctg gcggtcatgg
      181 gcatgatggc ggcgggcatt ttcctggtca cggcggtgaa cgccttccgc atgagcgccg
      241 gggatgccgc gcgcaaggac tccggcaccg gcggcttcac cctggtgggc gagtcttctc
      301 tgccggtgta tgaggacctg aacacgcagg cgggccggga cgccttcggc ctggatgagg
      361 agatcatgga aggcgtgcgc gtggcgccct tccgcgtgcg ccagggcgat gacgcgagct
      421 gcctgaacct gaaccgcgcg cagaagcccg tgctggcagg ggtgaacccg gcgctgctgg
      481 ccgggcgctt ccgctttgcc tcgggaaact gggaggcgct cgcagagcct caggccgcca
      541 tcgccgacca ggccaccgcc atgtggggcc tggggatcgg catgggagac gcgctggagt
      601 atcaggacgg cgcgggccgc gcatggagcg tgcggctcgc cgggatgctg gccggctccg
      661 tgctccaggg gaagatgatc ctcggcgagg acgctttttt gaaggcctac cctgacacgg
      721 ctggctaccg ctttttcctc atcgagaccg cgccggagcg cgcgggggag gtggccgcgc
      781 acctgacccg ccagctcgag aagcgcggcc tggcgctgga acccgcgcgc cagcgtctgg
      841 agacgtatct cagcgtgcag aacacctaca tcggcatctt cactgtgctt ggcggactcg
      901 gcgtgctgct gggcaccgcc ggcctgggca tcctcgtctc ccgtcatgtg ctggagagaa
      961 agggcgagct ggggctcatg caggcggtgg gcttcctgcc gggcgctctg cggcgcatgg
     1021 tgctggggga gcatgtcttc ctgctgctgg ccgggctgct gctcggggtg gtgagcgcgc
     1081 tgctggccgt gtggcccgct ctcaggcagg gcggcgggga tttgcccctg gcattcctgg
     1141 ggctgctgct ggccggggtg ctggctttcg gcatcctggc ctgcgcgctc gccgtgcagg
     1201 ccgctgtgcg cgggcgtctc atcgaggcgg tgcgcaggga gtaggcggac tgcgccaggg
     1261 ccgcgccggg cgcggctcga aattcgaatt tcgtactcct tgccaccgcg cgtttccccg
     1321 ccaaagaaaa ccccccgctc atgtccatct ggaaccacgc caacccgcag ctcaaagacc
     1381 tcgtcgccta cgaacccggc aaacccatcg aagatgtggc cagggaactc ggactgaggc
     1441 cggaggacat catcaagctg gcctcgaacg aaaacccgct gggtccaagc cccaaggccg
     1501 tcgaggcgat gaaacaggcg gtcgaggagg tccacatcta tccggacggc gcgtcttgga
     1561 agctgcgcaa cgccctggcg gagaagttcg gcctggaaat gggcaacttc atcatgggct
     1621 gcggcagcaa cgaggtcatc gagttcatcg gccacgcctt cctcaaaccc ggagacaaca
     1681 tcatcaccgc cgagcacgcc ttcctcgtgt acaagctcat ggcgaaggtc ttcggcgcgg
     1741 acaccatcga ggtgcccgat cccggttatg tgcatgatct tgacgcgatg gccgcggcgg
     1801 tcacgccgcg gacgaaggag gtcttcatcg cgaaccccaa caacccgacg ggcacgctcg
     1861 tcacgcagga gcagattgac cgcttcatgg acaaggtgcc ggagcatgtc gtcgtggtgt
     1921 ttgacgaggc ttattacgag ttcctcgaca acccgccgga cacgctcaaa tacgtccgcg
     1981 aagggcgcaa ggtggtcgtg ctgcgcacct tctcgaagat ccagggcctg gccggcacgc
     2041 gcgtgggcta cggcatcgcc aacaaggagc tcattgacgt gcttcagcgc acgcggcagc
     2101 ccttcaatct caactccatc gcccaggcgg gcgcgctggc cggcctgctc gacgaggagc
     2161 atcaggcgaa aaccaagcgc atcaccgatg agggccgcgc ctacctgcag aagcagtttg
     2221 gtgcgatggg tctcgaatac atcccgagcc acgccaactt cgtgctcgtc aaagtcggtg
     2281 acggcaacgc ggttttcaaa gcgatgatgg ccaagggtgt catcgtgcgc gccatggccg
     2341 cctacaagct gccggagtgg gtgcgcatct ccatcggcac catgccgcag aacgagcgct
     2401 gcatcgaggt gctcaaaggc gtgctgggcc gctgatgcgc ggggtcattc ctccgcgagc
     2461 gcttcgagca gatacctgga gggctggaag tgctcgatga gaatctcgtg ctgcgcgccg
     2521 cgtgtcatgc gcacctgctt gatgtcaaaa ttcggcgtct tgtgcggggc gaagaggatg
//
LOCUS       DAOMYJ010000422         2562 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Prosthecobacter sp. isolate AMR_MDS_5629
            NODE_15276_length_2562_cov_19.410850, whole genome shotgun
            sequence.
ACCESSION   DAOMYJ010000422 DAOMYJ010000000
VERSION     DAOMYJ010000422.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867850
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prosthecobacter sp. (wastewater metagenome)
  ORGANISM  Prosthecobacter sp.
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Verrucomicrobiaceae; Prosthecobacter.
REFERENCE   1  (bases 1 to 2562)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2562)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.30
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:34:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,742
            CDSs (total)                      :: 4,684
            Genes (coding)                    :: 4,676
            CDSs (with protein)               :: 4,676
            Genes (RNA)                       :: 58
            tRNAs                             :: 55
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2562
                     /organism="Prosthecobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15276_length_2562_cov_19.410850"
                     /isolate="AMR_MDS_5629"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1965333"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(36..548)
                     /locus_tag="PLP58_22640"
     CDS             complement(36..548)
                     /locus_tag="PLP58_22640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ11393.1"
                     /translation="MGEEEVAVAGAAGEEEAVEGGFAPVEGEVAAPLAGGGFEAGDLE
                     GQGVGADAGFQCGGDLDGDAGFFGEPAAEEDELGVSGGGRLPGAAVEAEAVQGDALAV
                     AAGGAGLGGGCDEPVALDAVDALRGEEGAAVEAAGFLMEEGVQAGLAAGAEAAGVGEG
                     AAVGEAIFGS"
     gene            675..968
                     /locus_tag="PLP58_22645"
     CDS             675..968
                     /locus_tag="PLP58_22645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ11394.1"
                     /translation="MPAAKLLDSPAPPSRTKRTPPAMSQLELFENNPPSTTGAILDIK
                     GETSNFVDNMRLPVHRWFRYSAGFSAEWVKSVLAERTRAPQRVLDPFVGSGTT"
     gene            990..2036
                     /locus_tag="PLP58_22650"
     CDS             990..2036
                     /locus_tag="PLP58_22650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ11395.1"
                     /translation="MPSIGLEAHPFVIRVARAKLGYSADSARFSQLAEQVILDAQQAP
                     TGTSAIPALMTKCFSEESFRSLDALRLAIEKADDSSAETSLLWLALVATLRPASEAGT
                     APWQYVLPGRRKTIPQDPFLLFRRTVATFAADMTVVSRRATPLARIEQDDARECRTVP
                     EGWATLVITSPPYANNYDYADATRLEMTFLREVTGWGDLQSAVRRHLVRACSQHVPPG
                     AVDMEAIISEPVLAPIREELARVCGQLSVLRMERGGKKTYNNMIACYFHDTARVWWAL
                     RRACADGAEVCFVIGDSAPYGVYVPVADWLGRLAVAAGFKDWTFEKLRDRNHKWKNRK
                     HRVPLCEGRLWVRG"
     gene            2052..2525
                     /locus_tag="PLP58_22655"
     CDS             2052..2525
                     /locus_tag="PLP58_22655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ11396.1"
                     /translation="MAQSLAHRWGQIIGDVFELFVERVLADAAAKHGLYLDKKGRRPA
                     RPGKKVTWHDGYGNKHDLDYVLERGGSASTLGTPVAFIESAWRRYTKHSKNKVQEIEA
                     AVSPVADTFSRHQPFRGAVLAGEFTHSALQQLESKGFALLHLPYASILTAPERRD"
BASE COUNT          463 a          960 c          718 g          421 t
ORIGIN      
        1 tcgcaagccg atcgctggca agaaatgatt agcaactagg acccgaagat ggcctcgccg
       61 acggcagcgc cttcacccac gccggcagcc tcggccccgg cggccagacc agcctgcacg
      121 ccctcttcca tcaaaaaacc ggcagcctcc acggccgcgc cctcctcacc cctgagagca
      181 tcaacggcat cgagagcgac tggttcatcg caccccccgc caagccccgc gccaccggca
      241 gctaccgcca gggcatcccc ctgcacagcc tcagcctcca cggcggcccc tggcagccgc
      301 ccgcccccgg acacacccag ctcatcctcc tcggcggcgg gctctccgaa gaagccggca
      361 tccccatcga gatcaccccc gcactgaaaa cccgcgtcag cgcccacccc ctggccctcc
      421 agatcaccgg cttcaaaccc gccaccggcc agtggagcgg ccacttcacc ctcgacgggc
      481 gcaaagcccc cctccacggc ctcctcctcc ccggcggcgc cggccacggc cacttcctcc
      541 tcccccaagc ccccgcccca ggagaaaaaa tcctcccccc ctggtccggc cgcgcccgaa
      601 tcgagccgtg aaaaacatgg cactgcggtg aatcgggtct tgcacaagca tgggcggcac
      661 tcactgtcag cgccatgccc gccgcgaaac tcctggactc ccccgctcct ccgagccgga
      721 ccaagcgcac cccgcctgcc atgtcgcagt tggagctttt tgaaaacaac ccgccttcca
      781 ccactggcgc gatcctcgac atcaagggcg agacctccaa ctttgtggac aacatgcggc
      841 tgcctgttca caggtggttc cgttattccg ccgggttttc cgccgaatgg gtgaagtccg
      901 tgctcgcaga aaggacccgc gcgccccagc gtgtgctcga ccccttcgtc ggctcaggaa
      961 cgacctgact ggcctgcgat gaaacgggcg tgccctccat cggactggaa gcgcatcctt
     1021 ttgtcatccg cgttgcccgc gccaaactcg gctattcggc ggactccgcc cgtttctcgc
     1081 agcttgcgga gcaagtcatt ctggatgctc agcaagcccc gacgggcacg tccgccatcc
     1141 cggcgctgat gaccaagtgc ttcagtgagg agtcattccg ctccctcgac gccctccggc
     1201 tcgccattga aaaagctgac gacagttccg cggagacctc cctgctctgg ctcgccctcg
     1261 tcgccaccct gcggcccgcg tccgaggctg gcacagcgcc gtggcagtat gtcctgccag
     1321 gacgccgaaa gaccattcct caggacccct tccttctctt tcggcgcacc gtcgccactt
     1381 ttgcggcgga catgaccgtc gtctcacgac gcgcgacccc gctggcgcgc atcgagcagg
     1441 acgacgcccg tgaatgccgc accgtgcccg aaggctgggc cactctggtc atcacttccc
     1501 cgccctatgc caacaactac gattacgcgg atgccacccg gctcgaaatg accttcctgc
     1561 gggaggtcac aggctggggt gatctccagt cagccgtgcg ccgtcatctc gtgcgcgcct
     1621 gctcccagca tgtgcctcct ggagctgtgg acatggaagc catcatttcc gagccagtcc
     1681 tcgcgcccat ccgggaggaa ctggcgcggg tgtgcgggca gttgtccgtg ctgcgcatgg
     1741 agcgcggcgg caagaaaacc tacaacaaca tgatcgcctg ctatttccac gacacggcgc
     1801 gggtgtggtg ggccctgcgc cgggcctgcg ccgacggggc ggaagtctgc ttcgtcattg
     1861 gcgactcggc gccctacggt gtctatgtgc ccgtcgccga ctggctgggc aggctggctg
     1921 tggcggcggg cttcaaagac tggaccttcg aaaaactgcg cgacaggaac cacaaatgga
     1981 agaaccgcaa acaccgggtg cccctctgcg agggtcgtct ctgggtgcgc ggctgaatct
     2041 gaatccgcgt catggcacaa tcactcgcac atcgttgggg acagatcatt ggcgacgtgt
     2101 tcgagctttt tgtcgagcgc gtccttgccg acgctgccgc gaaacacggc ctctatcttg
     2161 acaagaaagg cagacgcccg gcaaggcccg ggaaaaaagt cacctggcac gacggttacg
     2221 gcaacaagca cgacctggat tatgtgctgg agcgcggcgg ctccgcgtcc accctgggca
     2281 cgcccgtggc cttcatcgaa tccgcctggc gacgctacac caagcactcc aagaacaaag
     2341 tccaggaaat tgaggcggcc gtcagccccg tcgccgacac cttcagccgc caccagccct
     2401 ttcgcggcgc ggtgctcgcg ggcgagttca cccattccgc cctccagcag ctcgaaagca
     2461 agggcttcgc cttgctccac ctgccctacg cctccattct caccgcgccc gaacgccgcg
     2521 attgaagaaa gtcgttttgc gcggtgagaa cggtgcgatg gg
//
LOCUS       DAOMYJ010000423         2558 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Prosthecobacter sp. isolate AMR_MDS_5629
            NODE_15312_length_2558_cov_16.955254, whole genome shotgun
            sequence.
ACCESSION   DAOMYJ010000423 DAOMYJ010000000
VERSION     DAOMYJ010000423.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867850
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prosthecobacter sp. (wastewater metagenome)
  ORGANISM  Prosthecobacter sp.
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Verrucomicrobiaceae; Prosthecobacter.
REFERENCE   1  (bases 1 to 2558)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2558)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.30
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:34:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,742
            CDSs (total)                      :: 4,684
            Genes (coding)                    :: 4,676
            CDSs (with protein)               :: 4,676
            Genes (RNA)                       :: 58
            tRNAs                             :: 55
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2558
                     /organism="Prosthecobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15312_length_2558_cov_16.955254"
                     /isolate="AMR_MDS_5629"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1965333"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..376)
                     /locus_tag="PLP58_22660"
     CDS             complement(<1..376)
                     /locus_tag="PLP58_22660"
                     /inference="COORDINATES: protein motif:HMM:NF019589.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-hydroxyacyl-ACP dehydratase"
                     /protein_id="HRJ11397.1"
                     /translation="MLQPPSFVQALSALPHGPEFRFIDELLELEPGVSARARWTLKGT
                     ESFLPGHFPGEPLLPGVIMIEALAQLGGILAQSDRAERPLRDLRLTAVRQFKILGSAP
                     PGTVLEITARREAVMPGLVQVSG"
     gene            474..1280
                     /locus_tag="PLP58_22665"
     CDS             474..1280
                     /locus_tag="PLP58_22665"
                     /inference="COORDINATES: protein motif:HMM:NF013702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HRJ11398.1"
                     /translation="MDRFRLICAWLASLIYWVVGGIVFLLLCVLLPLFYRPERAREIA
                     QILLRASFQGFVRMMRFYDIVECEYEGFDRVREAKGPLILAPNHPALWDAVFVLAEAG
                     GMACVLKAGLMCNPILYGGATAAGFIPNEPAHKMLRQCIETLKRDGRLLFFPEGTRTR
                     PEHGLLNPFQGGLGIVAKNSGAPVWPVHVRASSPYLSKGWPLWKLPRGKIRIRMSVGE
                     PVCFLPGVTSRHFWTTCARATWPRWKSPLNEPPHPHPLLQHRPHPVPHCR"
     gene            1216..1989
                     /locus_tag="PLP58_22670"
     CDS             1216..1989
                     /locus_tag="PLP58_22670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009963600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HRJ11399.1"
                     /translation="MNPLILIPSYNTGPILSRTVADALAGWPQVWVVVDGSTDGSAAL
                     LDDLKAAHPGLRVLSLPRNQGKGSAVLHGAAEAAKAGFTHVLTLDADGQHPAGEIPRF
                     LALAEAHPQAALMGRPVFGPEAPQLRVQGRKLSNVWAHIETLGWGIPDSLFGMRLYPV
                     EALLRAFTGTAWARRFDYDTEIAVRLAWLGVPMLAVPTPVRYLTREEGGISQFRYLRD
                     NALLTWMHARLLCGFLLRLPRLLWLLGKGGNPLRRARAG"
     gene            2180..>2558
                     /locus_tag="PLP58_22675"
     CDS             2180..>2558
                     /locus_tag="PLP58_22675"
                     /inference="COORDINATES: protein motif:HMM:NF017026.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HRJ11400.1"
                     /translation="MNPRTPAKADFDRIARLCDRRWDYHYTRSKLRTDPVYAAVAAVI
                     AARPLPVLDIGCGIGLLGQYLRACGLDAPLTGFDYDERKIRAARVMAARAGHSDLNFE
                     AGDARTGLPEFSGHVVILDILQFF"
BASE COUNT          393 a          969 c          779 g          417 t
ORIGIN      
        1 ccccggacac ctggaccagg ccgggcatca ccgcctcacg ccgcgccgtg atctccagca
       61 ccgtgcccgg cggcgcgctg ccgagaatct tgaactggcg caccgccgtc aggcgcaggt
      121 cccgcagcgg gcgctccgcg cggtcactct gcgccagaat gccgccgagc tgcgccagcg
      181 cctcgatcat gatcacgccc ggcagcagcg gctcgccggg gaagtggccg ggcaggaagg
      241 actcggtgcc tttcagcgtc cagcgcgcgc gcgccgagac gcccggctcc agctccagca
      301 gctcgtcaat gaagcggaac tccggcccgt gcggcagcgc ggaaagtgct tggacaaatg
      361 aggggggttg caacatactc gcggcccttt ttacgcatga accggctcgc tgacaatggt
      421 caaagcccca gcccaaacgc cccccgagcg agcgcgcgcg cgggtcttcc ctcatggaca
      481 ggttccgtct gatctgcgcg tggctggcca gcctcatcta ctgggtggtc ggcggcattg
      541 tcttcctgct gctatgcgtg ctgctgccgc tgttctaccg gcccgagcgc gcgcgggaga
      601 tcgcgcagat ccttctgcgc gccagcttcc agggctttgt gcgcatgatg cgcttttacg
      661 acatcgtgga gtgcgagtac gagggctttg accgggtgcg cgaggcgaaa gggccgctca
      721 tcttggcgcc gaaccacccc gcgctgtggg acgccgtctt tgtgctggcc gaggccggcg
      781 gcatggcctg cgtgctcaaa gcgggcctga tgtgcaaccc catcctctac ggcggcgcca
      841 cggcggcggg cttcatcccg aacgagcccg cgcacaaaat gctgcgccag tgcatcgaga
      901 cactcaagcg ggacggccgt ctgcttttct tccccgaagg cacgcgcacc cgccccgagc
      961 acgggctgct caatcccttc cagggcggcc tcggcatagt cgccaaaaac tccggcgcgc
     1021 ccgtctggcc cgtgcatgtg cgcgccagca gcccctacct gagcaaaggc tggcccttgt
     1081 ggaagctccc gcgtgggaaa atccgcatcc gcatgagcgt gggcgagccg gtttgcttcc
     1141 tgcccggggt gacatccaga catttctgga cgacctgcgc gcgcgccacc tggccgcgct
     1201 ggaaaagccc gctgaatgaa ccccctcatc ctcatcccct cctacaacac cggccccatc
     1261 ctgtcccgca ctgtcgctga cgccctggcc ggctggccgc aggtctgggt cgtggtggac
     1321 ggcagcaccg atggcagcgc cgcattgctg gacgatctca aagcagccca ccccggcctg
     1381 cgcgtgctct ccctcccgcg caaccagggc aaaggctccg ccgtgctgca cggcgccgca
     1441 gaggcggcca aagcgggttt cacccatgtg ctcacgctcg acgcggacgg gcagcacccc
     1501 gccggggaaa tcccgcgctt cctggccctg gcggaggcgc acccgcaggc cgcactcatg
     1561 ggccggcccg tcttcggccc ggaggcaccg cagctccgcg tgcaggggcg gaagctctcc
     1621 aacgtctggg cacacatcga gaccctcggc tggggcatcc ccgactcgct cttcggcatg
     1681 aggctctacc ccgtggaggc gctgctgcgc gcctttaccg gcaccgcctg ggcgcgccgc
     1741 ttcgactacg acaccgagat cgccgtgcgc ctggcctggc tcggcgtgcc catgctcgcc
     1801 gtgcccaccc ccgtgcgcta cctcacccgc gaggagggcg gcatttccca gttccgctac
     1861 ctccgggaca atgccctgct cacctggatg cacgcccgct tgctctgcgg cttcctcctg
     1921 cgcctgccgc gcctgctctg gctgctgggc aaaggcggca accccctgcg ccgcgcccgc
     1981 gccggataac ccgaatccgg aaatgaatgg ccgcaaaaaa ctcaaaaaaa ccgcaagaaa
     2041 taaagcccgc atgctcctca cgcactcacg cgcgcggtga gggccccgcc gctttcccat
     2101 gtcttgtttt tgcgtctttt gtgtcttttt gcggccatcc catctccatt ttcagaaaga
     2161 accccgcgcc gcccatccca tgaacccgcg cacgccagcc aaagccgact tcgaccgcat
     2221 cgcccgcctg tgcgaccggc gctgggacta ccactacacc cgctcgaagc tccgcaccga
     2281 ccccgtctat gccgccgtgg ccgccgttat cgccgcgcgc ccgctgcccg tgctcgacat
     2341 cggctgcggc atcggcctgc tcggccagta cctgcgcgcc tgcgggctgg acgcgccgct
     2401 caccggtttc gactatgacg agcggaaaat ccgcgctgcc cgcgtcatgg ccgcgcgcgc
     2461 cgggcacagt gacttgaatt tcgaggcggg cgacgcgcgc accggcctgc ccgaattctc
     2521 cggccacgtc gtcatcctcg acatcctgca attcttca
//
LOCUS       DAOMYJ010000424         2549 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Prosthecobacter sp. isolate AMR_MDS_5629
            NODE_15395_length_2549_cov_17.734162, whole genome shotgun
            sequence.
ACCESSION   DAOMYJ010000424 DAOMYJ010000000
VERSION     DAOMYJ010000424.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867850
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prosthecobacter sp. (wastewater metagenome)
  ORGANISM  Prosthecobacter sp.
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Verrucomicrobiaceae; Prosthecobacter.
REFERENCE   1  (bases 1 to 2549)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2549)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.30
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:34:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,742
            CDSs (total)                      :: 4,684
            Genes (coding)                    :: 4,676
            CDSs (with protein)               :: 4,676
            Genes (RNA)                       :: 58
            tRNAs                             :: 55
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2549
                     /organism="Prosthecobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15395_length_2549_cov_17.734162"
                     /isolate="AMR_MDS_5629"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1965333"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..376)
                     /locus_tag="PLP58_22680"
     CDS             complement(<1..376)
                     /locus_tag="PLP58_22680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocation signal
                     domain-containing protein"
                     /protein_id="HRJ11401.1"
                     /translation="MGAAFERRAATNHSHPAMIRRISRRRLLQNACAASGAGLFSPGL
                     TAAPEGMRIAPFRFDVTPPAGHPLCGGWITPAVDVEDALEAIGFVLLGAGAPVVVCAV
                     DWTGILNEAHIAWRGALAEAAGT"
     gene            736..1626
                     /locus_tag="PLP58_22685"
     CDS             736..1626
                     /locus_tag="PLP58_22685"
                     /inference="COORDINATES: protein motif:HMM:TIGR00147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family lipid kinase"
                     /protein_id="HRJ11402.1"
                     /translation="MNGSIPVILNPAARSTQAAAREQAIRSLRPEPELFLTSAPGEAA
                     EIAERLARAGRPLIVAAGGDGTMNEVLQGVCKANATLPAGAKHTALGVLPLGTMNVFA
                     LELGLPSADIAACWRMISGGRFKEVDLWLAGEHYFVQLAGVGFDAEIIQETPWERKKK
                     FGPLSYVMSAMQVLTRKPPVLSVHAEGRPPLHGSVVLIGAGRHYGGPVPVFPKADNQD
                     GLLDVLIFRGLGGWEFAQMLRAILTGGYEPAEDIDYLQLREFTVTSEPAAPVELDGEL
                     SSGMTPVTFRPAAFKLQVAV"
     gene            complement(1629..>2549)
                     /locus_tag="PLP58_22690"
     CDS             complement(1629..>2549)
                     /locus_tag="PLP58_22690"
                     /inference="COORDINATES: protein motif:HMM:NF019223.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1553 domain-containing protein"
                     /protein_id="HRJ11403.1"
                     /translation="RENPLTARVTVNHIWARLFGIGLVHTPGDFGVRGERPTHPELLD
                     WLAAEFMERGWSRKQFLKTVLLSATYRQSSAAARPAAELARIQELDPNNSLLWRQNRL
                     RVEGEIVRDLHLAASGLLSPKIGGPSVFPPMPEDVAALSYANNFKWTTSKGGDRHRRG
                     MYTFFKRTSPHPDLMTFDCPDSNITNIQRTVSNTPLQALTTLNAETYAEAAQALAKRV
                     LTTPGLGNDEERLSHAFRLCLSRAPSAREIAALKKLLTESRYHYEASPDEAKAAVSLH
                     PAADIPPPENAAWTATARILLNMDEFITRG"
BASE COUNT          384 a          768 c          951 g          446 t
ORIGIN      
        1 tggtgcccgc cgcctccgcc agcgcgccgc gccaggcgat gtgcgcctcg ttgaggatgc
       61 ctgtccagtc caccgcgcac accaccaccg gcgcgccagc ccccagcagc acaaagccga
      121 tggcctccag cgcgtcctca acatccaccg ccggggtgat ccagccgccg cacagcggat
      181 gtcccgccgg cggtgtcaca tcaaagcgga aaggcgcgat gcgcatcccc tccggcgcgg
      241 ctgtgaggcc gggggagaaa agccccgcgc cactggcggc gcaggcgttt tggagcagac
      301 ggcggcggga gatgcggcgg atcatggcag gatggctgtg gttggtggcg gcgcggcgct
      361 caaaggccgc gcccatgcca aaacacgcct ccaaccgccc tttttttcca atgaacccgc
      421 gcatggattc tctggcgcgc gcctccgcga ggcggtttgc cgccggcggc tttcaatcgc
      481 tgccttgaag catgccgggc gtcatcacga tttgtgcttc ccttcttgga gtgttggagt
      541 tgtggagtga tgaatctcca atgctccagc actccatgac tccaactgcc ggtttcacaa
      601 tttgtggtga cgcgaggcat gacaccggca aaaccatgac cgcgtcagca ggcggcgcgc
      661 cggatgaaag ggccggtttt ttgcggcgcg ttcgggcttg agaaagcggg catcctgccg
      721 cataggcggc gcgccatgaa cgggagcatt ccagtcatac tcaatccggc ggcgcgcagc
      781 acccaagcgg cggcgcggga gcaggccatc cggtcccttc ggcctgagcc ggagcttttc
      841 ttgacctcgg ctccgggcga ggcggcggag atcgcggaac ggctggcgcg cgcgggccgg
      901 cctctcatcg tggccgcggg cggggatggc acgatgaacg aggtgctcca aggcgtgtgc
      961 aaggccaatg ccacgctgcc cgccggcgcg aagcacacgg cgctgggcgt gctgccgctg
     1021 gggacgatga atgtgtttgc cctggaactt ggcctgccct ccgccgacat cgccgcctgc
     1081 tggcggatga tctccggcgg gcgcttcaag gaggtggact tgtggctggc cggggagcat
     1141 tattttgtgc aactggcggg cgtgggcttc gacgcggaaa tcatccagga gacgccgtgg
     1201 gagcggaaaa aaaagttcgg cccgctgagc tatgtgatgt ccgccatgca ggtgctcacg
     1261 cgcaagccgc cggtgctctc ggtgcatgcg gaggggcgcc cgccgctgca tggctcggtg
     1321 gtgctcatcg gcgcgggcag gcattatggc gggccggtgc cggtgtttcc caaggcggac
     1381 aatcaggacg ggctgctgga tgtgctcatt tttcgcggcc tgggcggctg ggagttcgcg
     1441 caaatgctgc gcgccatcct caccggcggc tacgaacccg cggaggacat agactatctg
     1501 caactgcgcg agttcaccgt gacctcggag ccggccgcgc cggtggagct ggacggggag
     1561 ctttcctccg gcatgactcc ggtgaccttc cgcccggccg ccttcaagct gcaggtggcg
     1621 gtgtgagttc agccgcgcgt gatgaactcg tccatgttca gcaggatgcg cgcggtggcg
     1681 gtccaggcgg cgttttccgg cggcgggatg tccgccgcag gatggaggct gacggcggct
     1741 ttggcctcgt cgggacttgc ctcgtagtgg tagcgggact cggtgaggag tttcttcaac
     1801 gcggcgatct cgcgcgctga cggggcgcgg ctgaggcaga ggcggaaggc gtgggagagg
     1861 cgttcttcgt cgttgcccag gccgggcgtg gtgagcacgc gcttggcgag agcctgcgcg
     1921 gcctcggcgt aggtctcggc gttgagggtg gtgagggctt gcagcggggt gttggagacg
     1981 gtgcgctgga tgttggtgat gttcgagtcc gggcagtcaa aagtcatcaa gtccgggtgc
     2041 ggcgaggtgc gcttgaaaaa ggtgtacatg ccgcgccggt ggcggtcccc gcctttgctt
     2101 gtggtccact tgaagttgtt ggcgtagctg agcgcagcca catcctcggg catgggcggg
     2161 aagacgctgg ggccgccgat cttcggggac agcaggccgg aggcggcgag gtggaggtcg
     2221 cgcacgatct cgccctccac acgcaggcgg ttctggcgcc agagcagcga attgttcggg
     2281 tccagctcct gaatgcgcgc gagttcggcg gcggggcgcg cggctgcgct gctctggcgg
     2341 taggtggcgg agaggagcac ggtcttgaga aattgcttcc ggctccaacc gcgctccatg
     2401 aactcggcgg cgagccagtc gagcagttcg gggtgggtgg ggcgctcgcc gcgcacgccg
     2461 aagtcgccgg gtgtgtggac gaggccgatg ccgaacaggc gcgcccagat gtggttgacg
     2521 gtgacgcgcg cggtgagggg attttcccg
//
LOCUS       DAOMYJ010000425         2546 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Prosthecobacter sp. isolate AMR_MDS_5629
            NODE_15414_length_2546_cov_23.935367, whole genome shotgun
            sequence.
ACCESSION   DAOMYJ010000425 DAOMYJ010000000
VERSION     DAOMYJ010000425.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867850
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prosthecobacter sp. (wastewater metagenome)
  ORGANISM  Prosthecobacter sp.
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Verrucomicrobiaceae; Prosthecobacter.
REFERENCE   1  (bases 1 to 2546)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2546)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.30
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:34:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,742
            CDSs (total)                      :: 4,684
            Genes (coding)                    :: 4,676
            CDSs (with protein)               :: 4,676
            Genes (RNA)                       :: 58
            tRNAs                             :: 55
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2546
                     /organism="Prosthecobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15414_length_2546_cov_23.935367"
                     /isolate="AMR_MDS_5629"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1965333"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2016)
                     /locus_tag="PLP58_22695"
     CDS             complement(<1..2016)
                     /locus_tag="PLP58_22695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ11404.1"
                     /translation="MSENDAHVRPPADATASVHISANAQSSGLHAAALQSDGTVRVWG
                     KISPTPDEVRDVADLAASSGRVLAINANGEFFAWGSWSERADFIRRIQQGSHKIVQIA
                     AGAGYDYLLTDQGRILTRSSLADRQADIDANGPFQALVNGSGKAGAITLDGRLLELHT
                     PNNAKPNLKPLAFLRAQADAFALNHETVIASKDGKLVGAVPESQASSYAGVTRIVADS
                     SRIQAWRMADGAWKFVTGASSGTISIPHCEQQAEGCFDIALAGDYAFGLKAEADSSLA
                     GASTSNLPSAPSTPVPAAFPPGQWVKVFTKFEDLPDSLRQPGRGVKFEDGWIRFEPGK
                     PLRIYVVDSLTNYAIRATMQRAGDEYKSKSLMIRAAHGTSIDGYLLHVPNDNSFFGAG
                     RRVNGGTATTYLWKALPHEFQPPRIGQEYTLEWGCVGDKLIARYGTHFVKTVINTDLK
                     SGPAYLTGEEPVRDIEAINLDGLPEAEALKLLGVDEKGNDLRALAAQQERQQMEQSQK
                     DDALAAIPELKALHEQFVKLQAERVTAPFEAEVAKLNASYLGGIDREIANEKKAGHLD
                     GVIALESEKKIIADKQPVPAADDAKTPEALKKLRAIYRDTHAKIDASRAANLKALTDP
                     LITRLKQLVAELTRQDRVAHAKTVREYREALEAGRPAAPPAAASGPAA"
BASE COUNT          399 a          748 c          857 g          542 t
ORIGIN      
        1 ggccgcgggg ccgctcgcgg cagctggggg ggcggccggc ctgccagctt ccagcgcctc
       61 ccgatactcc cgcacggtct tggcatgggc gacgcggtcc tgccgggtga gctcggcgac
      121 gagctgcttc aaccgcgtga tgagcgggtc ggtgagcgcc ttgagattcg cggcgcggga
      181 ggcgtcaatc ttcgcgtggg tgtcacggta gatggcgcgg agtttcttga gcgcctcggg
      241 cgtcttcgca tcgtccgcgg caggcacggg ctgcttgtct gcgataatct tcttctctga
      301 ctccagagcg atgacaccgt caaggtgccc ggccttcttc tcattggcga tctcgcggtc
      361 gatgccgccg agataactcg cattgagctt cgccacctcc gcctcgaagg gcgcggtgac
      421 gcgctcggcc tgcagcttca cgaactgctc gtgcagcgcc ttgagttccg ggatggcggc
      481 gagggcatcg tctttctgcg actgctccat ctgctgccgc tcctgctgcg cggccagcgc
      541 gcgcagatca ttgcccttct catccacgcc gaggagtttg agcgcctccg cttcgggaag
      601 gccgtcgagg ttgatggctt cgatgtcgcg gacgggttct tctccggtga ggtatgccgg
      661 gccgctcttc aagtcagtgt tgatcaccgt tttaacgaaa tgggtcccgt aacgggcgat
      721 gagcttgtca ccgacacaac cccattccag cgtgtattcc tgtccgatgc gaggtggctg
      781 gaactcatga ggcagcgcct tccacaagta ggtggtggcg gttcctccat tcaccctgcg
      841 gcccgccccg aagaacgaat tgtcgttcgg gacgtgaagc aagtaaccgt cgatgctggt
      901 tccatgtgcg gcacggatca tgagggactt gctcttatac tcatcgcctg cacgctgcat
      961 cgtggcgcga atcgcatagt tggtgagcga gtccacgacg taaatgcgca acggcttgcc
     1021 tggttcgaag cggatccacc cgtcttcgaa cttcacgcct cttcccggct ggcgcagaga
     1081 gtccggcaag tcctcgaact tcgtgaatac cttcacccac tggccgggcg ggaaggccgc
     1141 cgggacggga gttgaagggg cggatggaag atttgaggtg gaggcgccag cgagcgaaga
     1201 gtccgcctct gctttcagac cgaaggcgta gtcgccagcc agggcgatgt cgaagcagcc
     1261 ttcggcttgc tgctcgcagt gcgggatgct gatcgtgccc gatgatgccc cggtgacgaa
     1321 tttccacgca ccgtcggcca tcctccaggc ctggatgcgg ctgctgtctg ctacaatccg
     1381 ggtcactccg gcgtagctgg atgcctgaga ttccggcaca gcgcctacca acttgccgtc
     1441 cttggaagcg atgaccgttt cgtgattcag cgcgaaggca tctgcctggg cgcgcaggaa
     1501 ggcgagaggc ttcaagtttg gcttggcatt gttgggtgtg tgaagttcca gaaggcgtcc
     1561 gtccagcgtg atggccccgg cttttccaga gccattgacg agagcctgga aagggccgtt
     1621 ggcatcgatg tcggcctggc gatctgccag actgctccgc gtcaggatcc ggccttggtc
     1681 cgtgagcagg taatcgtaac ccgcccctgc ggcgatttgg acgattttgt gactcccttg
     1741 ctgaatgcgg cggatgaagt cagctctttc actccaactg ccccaggcga aaaattcacc
     1801 gtttgcgttg atggcaagga cgcgccccga agaggccgcg aggtcggcaa cgtctcgaac
     1861 ctcatcgggg gttggcgaaa ttttgcccca aacccgcacg gtgccgtcgg actggagggc
     1921 ggcggcgtgc aacccgcttg actgtgcatt cgcggagata tgcacgctcg ccgtcgcgtc
     1981 cgcaggcggc ctgacgtgcg cgtcattctc gctcatgggc ttgccatcaa cgcgccagac
     2041 gacaatctgc ccgacttggg ttggtcttgc gggcgccttg agcggcaagg tcgtctggac
     2101 gggttgtggc gtaggcagga tcgaagacgc gactgttgct gatttgccag actgcgggcg
     2161 tatcgtggct gtcggttgaa tgttatttgc ggtgctgctt gatttggggg tggtgccgct
     2221 gccagtcgtt gtagaactgg taagagttga tggcgaagat tgagaaggtc tgggtgcggg
     2281 tttggcagcg ctgggggccg gggtggaagg cttggtgggc tcctttttcc cgctcatcat
     2341 gacgaacgct ccgatgccga tggcggcgat ggcgccaatg ctgatgaaga gcggtgcttt
     2401 ggacttcgcg gatgggtcgg aagtggtgcg ggcactcttg cccgcgtgag cgccttcttt
     2461 gcgggcagga gtgcccacat cactcctccc cgccggcctc tgcgcggcgc tgcgctggcc
     2521 gggggcctgc gccacctggg ccacgg
//
LOCUS       DAOMYJ010000426         2538 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Prosthecobacter sp. isolate AMR_MDS_5629
            NODE_15483_length_2538_cov_20.215868, whole genome shotgun
            sequence.
ACCESSION   DAOMYJ010000426 DAOMYJ010000000
VERSION     DAOMYJ010000426.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867850
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prosthecobacter sp. (wastewater metagenome)
  ORGANISM  Prosthecobacter sp.
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Verrucomicrobiaceae; Prosthecobacter.
REFERENCE   1  (bases 1 to 2538)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2538)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.30
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:34:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,742
            CDSs (total)                      :: 4,684
            Genes (coding)                    :: 4,676
            CDSs (with protein)               :: 4,676
            Genes (RNA)                       :: 58
            tRNAs                             :: 55
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2538
                     /organism="Prosthecobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15483_length_2538_cov_20.215868"
                     /isolate="AMR_MDS_5629"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1965333"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..587)
                     /locus_tag="PLP58_22700"
     CDS             complement(<1..587)
                     /locus_tag="PLP58_22700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ11405.1"
                     /translation="MRQGCSTFIPRSCHEPGGFGYEWALARGEQPLELPAAAAGYRHY
                     QDGSREWVTWEQLHAARTARQAERLAAKLWPAWHVYERRLRPAQVWVFDNEGGADGVL
                     HAGTYCYLRWQQDGLACTPAMERRRGIRAQGSHAALALHLMRVIPLEDLLLLPENFED
                     WKEAFMKAYPKVRTRNDPRRAGVLSGWSDGVWWERR"
     gene            complement(584..895)
                     /locus_tag="PLP58_22705"
     CDS             complement(584..895)
                     /locus_tag="PLP58_22705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ11406.1"
                     /translation="MRGGYVHVEIRTPEGALVLTADVGLLHREVVQLAARSGWEQEAA
                     LSVAGAVLAHELDRAKTVAGECPPSHGQPVGAGDVPAGDEQVSVSHFLSPSGEPGRRS
                     A"
     gene            complement(892..1164)
                     /locus_tag="PLP58_22710"
     CDS             complement(892..1164)
                     /locus_tag="PLP58_22710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ11407.1"
                     /translation="MSSGLYTVAHVARYMGKRPQEVARLIDEDGLPAVELPGPKRPVR
                     KIALHGLHKWLAERAVGGAFMSVTELAREMAGVASGDRGVGEEVAA"
     gene            complement(1161..1502)
                     /locus_tag="PLP58_22715"
     CDS             complement(1161..1502)
                     /locus_tag="PLP58_22715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ11408.1"
                     /translation="MSIPMKHAADFTTHARRHGLWLSNVGARAVSQLMDMREAPVVPL
                     TGRDRHNKFWRETRPALGVLIRHGLAVWAGGQYEITAQGADYLLKLEAAGLLAGVPKI
                     REIRAEKRRAA"
     gene            complement(1499..1933)
                     /locus_tag="PLP58_22720"
     CDS             complement(1499..1933)
                     /locus_tag="PLP58_22720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ11409.1"
                     /translation="MKRAWLTNHGAELECSAGARLLQECRVALREWLASPARSELLPR
                     FMELRGELSWVAPEKMLRMPGLSFLSAYPDRVTLLYDGCPECGNLALEALTWESEGSA
                     ALTVGCWDCGWHLGPGGEVRQAPDNPMPHPENENQLLEEDEA"
     gene            complement(2253..>2538)
                     /locus_tag="PLP58_22725"
     CDS             complement(2253..>2538)
                     /locus_tag="PLP58_22725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ11410.1"
                     /translation="LRRQAAAPALLPVRDAREQWRAAGHDSALQRVCPRTGLYLVDGR
                     GGRCLMARDEWELYRGLCHAAHEPVDRALAGAVKTDAAARRGSTTEGGAA"
BASE COUNT          412 a         1034 c          691 g          401 t
ORIGIN      
        1 cgccgctccc accacacccc gtccgaccac cccgagagca cacccgcgcg gcggggatca
       61 ttccgcgtcc gcactttcgg gtaggccttc atgaaggcct ccttccagtc ctcgaaattt
      121 tcgggcagca aaagcagatc ctccagcggg atcacccgca tcagatgcag cgccagtgcc
      181 gcatgcgagc cctgcgcgcg gatgccccgg cgccgctcca tcgcgggcgt gcaggccagc
      241 ccgtcctgct gccagcgcag gtagcaataa gtgcccgcgt ggagaacccc atccgcgccg
      301 ccctcgttgt cgaagaccca cacctgagcc ggcctcagcc gccgctcata gacgtgccac
      361 gccggccaca gcttcgccgc cagcctctcc gcctggcgcg ccgtgcgcgc cgcatggagt
      421 tgctcccatg tgacccactc gcggctgcca tcctggtagt gccggtagcc agccgcggca
      481 gccggcagct ccagcggctg ctcgccgcgc gccagcgccc actcatagcc aaacccgcca
      541 ggctcatggc agctgcgcgg gatgaacgtc gagcaaccct gtctcacgcc gacctccttc
      601 ccggctcgcc gcttgggctt aaaaaatgac tcacggacac ttgctcgtca cctgcgggca
      661 catccccagc tccgacaggc tgtccgtgag agggcgggca ctcgcccgcc acagtttttg
      721 cgcggtccag ctcatgcgcc agcaccgccc ccgccaccga gagagccgcc tcctgctccc
      781 agccgctgcg cgcagccagt tgcaccacct cgcggtgcag cagacccaca tccgccgtca
      841 gcaccaatgc gccctccggc gtccggatct ccacatgcac atacccgccc ctcatgccgc
      901 cacctcctct cccacaccac gatcaccgct cgccacgccc gccatctctc gcgccagttc
      961 ggtcaccgac ataaatgcac cgcccacagc acgctccgcc agccatttgt gcagcccgtg
     1021 cagcgcaatt tttctgaccg gccttttggg ccccggcagc tccaccgccg gcagcccgtc
     1081 ctcgtcaatg aggcgcgcca cctcttgcgg ccgtttcccc atgtagcgcg ccacatgagc
     1141 caccgtgtat agcccgctgc tcatgccgcc ctccttttct ccgcgcggat ctcgcgtatt
     1201 ttcggcacac cggccagcag ccccgccgcc tccagcttga gcaggtaatc cgcgccctgg
     1261 gcggtgatct cgtattgccc gcccgcccac accgccagcc cgtgccggat cagcacaccc
     1321 agcgccggcc gcgtctcgcg ccagaattta ttatgccggt cacgacccgt cagaggcacc
     1381 accggcgcct cgcgcatgtc catgagctgc gacaccgcgc gcgcgcccac gttgctcaac
     1441 caaaggccat gccggcgggc atgtgtcgta aaatccgccg catgcttcat agggatgctc
     1501 atgcctcatc ttcctccagt agctgatttt cgttttcggg gtggggcatg ggattgtccg
     1561 gagcctggcg cacctcgcca cccggcccca ggtgccaccc gcagtcccag cagcccaccg
     1621 tcagagccgc gctgccctcg ctctcccacg tcagcgcctc cagcgccagg ttgccgcact
     1681 ccgggcagcc gtcatacagc agcgtcacgc ggtcgggata cgcgctcaga aaagacaggc
     1741 cgggcatgcg cagcatcttc tctggtgcca cccagctcag ctcgccacgg agttccataa
     1801 atcgcggcag caactcgctc cgcgctggcg acgccagcca ctcgcgcaac gccacccggc
     1861 actcctgcag cagccgcgcg cccgccgagc actccagctc agcgccgtga tttgtcagcc
     1921 aggcccgctt catgccgccc tcctttccgc cgcgcgcaga tcccgcacgt ccccacatac
     1981 atcccggcgg ttcagcgcct ggttcggcct tgcgcgccat ttctcagcgg cttcttgcac
     2041 gcttttggcg accgggccga tagcaaagca ctcgatattg gcgcatcgaa tgcaccagag
     2101 gcgcggacca acgccatagc cagactgaca gaaactcaga ggatccgttg gagttccgca
     2161 aaaccgacaa tcaccgaagg ccgaaccatg cttttcctcc aggtccgcga gcgtgcccag
     2221 ctcagcgccg tgatttgtca gccaggcccg cttcatgccg cccccccttc cgtggtcgag
     2281 ccgcgccgcg cggcggcgtc cgtttttacc gcgcccgcca gtgccctgtc caccggctca
     2341 tgagccgcgt ggcagagtcc acgataaagc tcccactcat cacgcgccat caggcagcgt
     2401 ccgccgcggc cgtccacgag gtagagaccc gtgcgcgggc acacccgctg cagcgcgctg
     2461 tcatggcccg ccgcgcgcca ctgctcccgc gcgtcacgca caggcagcag agcaggcgcc
     2521 gcagcctgcc gccgcagc
//
LOCUS       DAOMYJ010000427         2523 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Prosthecobacter sp. isolate AMR_MDS_5629
            NODE_15613_length_2523_cov_15.287682, whole genome shotgun
            sequence.
ACCESSION   DAOMYJ010000427 DAOMYJ010000000
VERSION     DAOMYJ010000427.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867850
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prosthecobacter sp. (wastewater metagenome)
  ORGANISM  Prosthecobacter sp.
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Verrucomicrobiaceae; Prosthecobacter.
REFERENCE   1  (bases 1 to 2523)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2523)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.30
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:34:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,742
            CDSs (total)                      :: 4,684
            Genes (coding)                    :: 4,676
            CDSs (with protein)               :: 4,676
            Genes (RNA)                       :: 58
            tRNAs                             :: 55
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2523
                     /organism="Prosthecobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15613_length_2523_cov_15.287682"
                     /isolate="AMR_MDS_5629"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1965333"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1567)
                     /locus_tag="PLP58_22730"
     CDS             complement(<1..1567)
                     /locus_tag="PLP58_22730"
                     /inference="COORDINATES: protein motif:HMM:NF015722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formylglycine-generating enzyme family protein"
                     /protein_id="HRJ11411.1"
                     /translation="MNFRLHAACLLLLLLCAGCSKPVIEGRVDFSPADALEWASAPLE
                     VGVLTEPELEKTARAALLAFREDAARLAAENAALQAQLGALDQARKALEARAKVLVSD
                     EGLGRHREGPLKIILEQTERIETLARGVEARHPGGFPAPFESRLLAALPSGSAKTGPD
                     GSFRLPPPRGPAWVMARAANRGEDEEHWWLLPATPGQALDLKTENAGGKALAAWVKEH
                     LPELLEPAPEPETAVAEEVRALAAASAAAVDAEINRVRALAAEERQKAEMAAKAEMEE
                     TARKMAAERAKAEAEAREKKHAEMMAAIAKPAREFLPGLKMLPVKAAAFTMGSPESEL
                     GRSANEGAVEARITRGFLLGRTEVTQAQWMLVMGDNPSEFRGATNPVEKVSWLEAVEF
                     CEKLTAKARAEGLLPEGWRFSLPTEAQWELACRAGAATALYSGKNLVSEKGPDKGLAE
                     IAWYHANSDQKTQAAGLKPANELGLHDMLGNVWEWCLDSHTPALQGGDDPAQVGDAKD
                     RVVRGGSWSQLPRG"
     gene            1755..>2523
                     /locus_tag="PLP58_22735"
     CDS             1755..>2523
                     /locus_tag="PLP58_22735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ11412.1"
                     /translation="MPPNHELALHLLRAAFWRSLGVWALFTFTCAAQDSRPSPVAGDS
                     IKVNFPNSPVQAIIPFYTEITGKKIILDSALQGEPLRIVAPQPLSRKEAIAFIEATLL
                     LNGYAIIPVDDSTVKLIHHTGGKNPASHGLKVYTAIHDLPQNEEICHFVLPLQHISPD
                     EASKAFQQVIKLNSFGAITPVTNAAALIITENSITIRSICEIAKVIDVPPAEIANEMI
                     KLERSDAESIAEIISEIYEERENAEAAAAPAPAPAAPR"
BASE COUNT          424 a          969 c          707 g          423 t
ORIGIN      
        1 agccgcgcgg gagctggctc caggagccgc cgcgcaccac gcggtctttg gcatcaccca
       61 cctgcgcggg gtcgtcgcca ccctggagcg ccggcgtgtg gctgtccagg caccactccc
      121 agacattgcc gagcatgtcg tgcagaccga gttcgttggc gggtttcagt cccgcagcct
      181 gggttttctg gtccgagttg gcgtgatacc aggcgatttc cgccagcccc ttgtccggcc
      241 ccttctccga gaccaggttt ttgccggagt aaagcgccgt ggcggcgccc gcccggcagg
      301 ccagctccca ttgcgcctcg gtgggcaggg agaaacgcca gccctccggc agcaggcctt
      361 ccgcgcgtgc cttggcggtg agtttttcac agaactccac cgcctccagc caggacacct
      421 tttccaccgg attcgtcgcg ccgcggaatt cgctgggatt gtcccccatc accagcatcc
      481 actgcgcctg agtcacctcc gtacggccga gcaggaagcc gcgggtgatg cgcgcctcca
      541 ccgcgccctc gttggcgctg cgaccgagct cactttccgg actgcccatt gtgaaggccg
      601 ccgccttcac cggcagcatt ttcagcccag gtaaaaactc gcgcgcgggt ttggcgatgg
      661 ccgccatcat ctccgcgtgt tttttctccc gcgcctcggc ctccgccttc gcgcgctcgg
      721 cggccatttt ccgcgccgtc tcctccatct ccgccttggc ggccatctcg gctttttggc
      781 gctcctccgc agccaaggcg cggacgcggt tgatctcggc atccacggcg gcggcgctgg
      841 cggcggccag ggcgcgcact tcctccgcca cagcggtctc cggctccggc gcgggctcca
      901 gcaactccgg caggtgttcc ttcacccagg ctgccagcgc cttgcctccg gcgttttccg
      961 ttttcaaatc cagcgcctgg cccggcgtgg cgggcagcag ccaccagtgc tcctcgtcct
     1021 ccccgcgatt cgcggcgcgc gccatcaccc aggccggccc ccgcggcggc ggcaggcgga
     1081 aggagccgtc cggtcccgtt ttggcactcc cagacggcag cgcggcaagc agccggctct
     1141 caaaaggcgc cgggaatccg cctggatgac gagcctccac cccgcgcgcc agcgtctcga
     1201 tgcgctccgt ctgctcgaga atgattttca acggcccctc acggtgacgg cccagcccct
     1261 cgtcggagac cagcaccttg gcgcgcgcct ccagcgcctt gcgcgcctga tccagcgcgc
     1321 cgagctgcgc ctgcagggcc gcgttttccg ccgccagccg cgcggcatcc tcccggaaag
     1381 ccagcaaagc ggcgcgcgcc gtcttttcca gctccggctc cgtcagcact cccacttcca
     1441 acggcgcgga ggcccattcc agcgcatccg ccggggaaaa atccacccgc ccctcgatca
     1501 ccggcttcga gcagcccgcg cagagcaaca gcagcagcag gcaggcggcg tggaggcgga
     1561 aattcatgcg aagcacgggc ggaggacggg gaatgtctgg cgcgaatgaa tggcctttcc
     1621 ccgcgcttgc aagcccgctc taatccctgc cacgcgccaa atccagcgag gaaagctcga
     1681 cggctctgaa ttttaagaac gccaaacaat tcagttgccg gaaaatcggt ttaaaattat
     1741 ggtttatata attcatgccg ccaaatcacg aactcgccct gcatcttctg cgcgccgcct
     1801 tctggcgaag cctgggggtt tgggcgcttt tcaccttcac ctgcgccgcc caggacagcc
     1861 ggccaagccc cgtcgctggc gactccatca aggtcaattt ccccaattcc cccgtccagg
     1921 ccatcatccc cttctacacg gagatcacgg ggaagaagat catcctcgac tccgccctcc
     1981 agggcgagcc cctgcgcatc gtcgcccccc agccgctctc gcgcaaagag gccatcgcct
     2041 tcattgaggc cacccttttg ctcaacggct acgccatcat ccccgtggat gacagcaccg
     2101 tgaagctcat ccaccacacc ggcggcaaaa accccgcctc ccacggcctc aaggtctata
     2161 ccgccatcca cgacctgccc cagaacgagg aaatctgcca cttcgtcctc ccgctccagc
     2221 acatctcccc ggacgaggca tcgaaggcct tccagcaggt catcaagctc aacagcttcg
     2281 gcgccatcac ccccgtcacc aacgccgccg ccctcatcat caccgaaaac agcatcacca
     2341 tccgcagcat ctgcgagatc gccaaagtca tagacgtgcc gcccgccgag atcgccaacg
     2401 agatgatcaa gctcgagcgc agcgacgccg aaagcatcgc cgaaatcatc tccgagatct
     2461 acgaggagcg cgagaatgcc gaggccgccg ccgcgcccgc tccggcgccc gccgccccgc
     2521 gac
//
LOCUS       DAOMYJ010000428         2504 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Prosthecobacter sp. isolate AMR_MDS_5629
            NODE_15793_length_2504_cov_13.783177, whole genome shotgun
            sequence.
ACCESSION   DAOMYJ010000428 DAOMYJ010000000
VERSION     DAOMYJ010000428.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867850
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prosthecobacter sp. (wastewater metagenome)
  ORGANISM  Prosthecobacter sp.
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Verrucomicrobiaceae; Prosthecobacter.
REFERENCE   1  (bases 1 to 2504)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2504)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.30
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:34:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,742
            CDSs (total)                      :: 4,684
            Genes (coding)                    :: 4,676
            CDSs (with protein)               :: 4,676
            Genes (RNA)                       :: 58
            tRNAs                             :: 55
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2504
                     /organism="Prosthecobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15793_length_2504_cov_13.783177"
                     /isolate="AMR_MDS_5629"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1965333"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2161)
                     /gene="rnr"
                     /locus_tag="PLP58_22740"
     CDS             complement(<1..2161)
                     /gene="rnr"
                     /locus_tag="PLP58_22740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009960828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008997 - ribonuclease R activity
                     [Evidence IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HRJ11413.1"
                     /translation="MQSKILKLLARPGYKPANIPELLQQLRLPPNQQQELQRALAGLL
                     AEGRVVRTKGNRHILAAEADLVPGVIQITRGGRGFVQPDEPGRPEIGIPENATATAIH
                     GDRVLVRLDVKPQGLRKSAAPGEVSGAVVRVLERARSRFVGTLHRGKKFLHVTPDDPR
                     LPSAIYVPEPRDVGRPARIGDKVVVEILGWEARGVPPEGEVIEVLGAPDEEGVDMLSV
                     LRHYDLPLHFPKPVLAEASAIARSRPDNQPAPEERAGRVDCRAHPVITIDPDDAKDFD
                     DAICVQRQDGQWRLWVHIADVSHYVRPGSALDTEARRRGNSTYLVDRVIPMLPEALSN
                     ELCSLKPGLDRLTKCVEFVLSGDGKVVSARFYPAVIHSRRRFSYQEAFACIQRAPRDS
                     LERMLHDAHTLAQRVRKLRFANGSLDLDFPENKIRLDGQGRVLRIERMENDASHQLIE
                     EFMLLANEAVAGRLLETNRPAVHRIHEPPKEKRLNDYREEVLSHGVRCGNLTHRPEVQ
                     KLLAKLNTLPIGQALKIGFLKSLMRARYSTEPLGHYGLAKKKYTHFTSPIRRYADLVV
                     HRALFEKIRLQNSEAKKIADHISTTERNSADAERDSRDVKLHAYLNAQIESGEPHTYR
                     ALVTDVRNFGFFVDVPDLGMSGLVPLSAIPDDFFLFDPARGHLVGRRTRRVIRLGDEM
                     PVQIHKVDSASKRVDFKPADAPAQKHGRGKPQPSKPGG"
BASE COUNT          374 a          771 c          928 g          431 t
ORIGIN      
        1 gtccgccggg tttggagggc tgcggtttgc cgcggccgtg cttttgcgcg ggcgcgtcgg
       61 cgggcttgaa gtccacgcgc ttgctggcgc tgtccacttt gtgaatctgc acgggcatct
      121 cgtcgccgag gcggatgacg cggcgggtgc ggcggccgac gagatggccg cgcgcggggt
      181 cgaagaggaa gaagtcgtcc gggatggcgg agaggggcac gaggccgctc atgccgaggt
      241 cgggcacgtc cacaaagaag ccgaagttcc gcacatcggt gacgagcgcg cggtaggtgt
      301 gcggctcgcc ggactcgatc tgcgcgttga ggtaggcgtg gagtttgaca tcgcggctgt
      361 cgcgctcggc gtcggcgctg ttgcgctcgg tggtggagat gtggtcggcg atctttttcg
      421 cctcgctgtt ctggaggcgg attttttcaa aaagcgcgcg atgcacgacg aggtccgcgt
      481 agcggcggat gggggaggtg aagtgggtgt actttttctt ggccagtccg tagtgcccca
      541 gcggctcggt ggagtagcgg gcgcgcatga gggattttaa aaagccgatc ttcagcgcct
      601 ggccgatggg aagggtgttc agcttggcga gcagtttttg cacttcgggc cggtgcgtca
      661 ggttgccgca gcgcacgccg tggctgagca cttcctcgcg gtagtcgttg aggcgctttt
      721 ccttcggcgg ctcgtggatg cggtggacgg cggggcggtt cgtctccagc agccggcccg
      781 ccacggcctc gttggccagg agcatgaact cctcgatgag ctggtgggag gcgtcgtttt
      841 ccatgcgctc gatgcgcagc acgcggccct gcccgtcgag gcggattttg ttttcgggga
      901 agtcgaggtc cagcgagccg ttggcgaagc gcagcttgcg cacgcgctgc gccagggtgt
      961 gcgcgtcgtg cagcatccgc tccagcgaat cccgcggcgc gcgctggatg caggcaaaag
     1021 cctcctgata gctgaagcgg cgccgcgagt ggatgaccgc tgggtaaaac ctggcgctga
     1081 cgactttgcc gtcgccgctc agcacgaact cgacgcactt cgtcagacgg tcgaggcccg
     1141 gcttgagcga gcacagctcg ttgctcaagg cctccggcag catgggaatg acacggtcca
     1201 cgaggtaggt ggagttgccg cgccggcgcg cctcggtgtc cagcgcgctg ccggggcgga
     1261 cgtaatggga gacatcggcg atgtggaccc acaagcgcca ctggccgtcc tggcgctgca
     1321 cgcagatggc gtcgtcgaag tccttggcgt catccgggtc aatggtgatg accggatgcg
     1381 cgcggcagtc cacgcgcccc gcgcgctcct ccggcgcggg ctggttgtcg ggccgcgagc
     1441 gggcgatggc gcttgcctcg gcgagcacgg gcttggggaa gtgcagcggc aggtcgtaat
     1501 gccggagcac ggagagcatg tccacaccct cctcgtcggg cgcgccgagg acttcgatga
     1561 cctcgccctc gggcggcacg ccgcgcgcct cccagccgag gatctcgacg acgaccttgt
     1621 ccccgatgcg cgcggggcgg cccacatcgc gcggctcggg cacataaatg gcggagggca
     1681 ggcgcggatc gtccggcgtg acgtggagga attttttccc ccggtgcagc gtgccgacaa
     1741 agcggctgcg cgcacgctcc agcacgcgca ccacggcgcc gctcacctcg ccgggcgcgg
     1801 cgcttttgcg caggccctgc ggcttcacat ccaggcgcac cagcacgcgg tcgccgtgga
     1861 tggcggtggc ggtggcgttt tcagggatgc cgatctccgg gcggccaggc tcgtcgggct
     1921 ggacaaagcc gcgcccgccg cgggtgatct ggatgacgcc cggcaccagg tcggcctcgg
     1981 cggcgaggat gtggcggttc cccttcgtgc gcacgacgcg gccttcggcc agcaggccgg
     2041 cgagcgcgcg ctgcagctcc tgctgctggt tcggcgggag gcggagctgc tggagcagtt
     2101 cggggatgtt ggcgggcttg taaccgggcc gcgcgagcag cttgaggatt ttcgattgca
     2161 tggccgcacc ctggcacagg cggaccgggc ggccaaggcg gattcatgcg cgcggcggga
     2221 atcgctccag ggactccgca actggagttc aacgccaagg ccagggcgtc agattgtcat
     2281 gatcacgcgc cgctctcacc cgtcgaaata cagagcagcg gcacctcatt gaaaggacct
     2341 ctgaaaagaa tcccggccat gcttgcgaac gcctcggatc aggcgacggc gagcgcaaga
     2401 cgttgctgac acgacagata gccttcgagc cgttgcctgc atccgttttg ttccagggag
     2461 tccgcacccg cgaagcggaa aacagcgccg tcaagccccc cggc
//
LOCUS       DAOMYJ010000429         2486 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Prosthecobacter sp. isolate AMR_MDS_5629
            NODE_15975_length_2486_cov_23.442205, whole genome shotgun
            sequence.
ACCESSION   DAOMYJ010000429 DAOMYJ010000000
VERSION     DAOMYJ010000429.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867850
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prosthecobacter sp. (wastewater metagenome)
  ORGANISM  Prosthecobacter sp.
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Verrucomicrobiaceae; Prosthecobacter.
REFERENCE   1  (bases 1 to 2486)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2486)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.30
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:34:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,742
            CDSs (total)                      :: 4,684
            Genes (coding)                    :: 4,676
            CDSs (with protein)               :: 4,676
            Genes (RNA)                       :: 58
            tRNAs                             :: 55
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2486
                     /organism="Prosthecobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15975_length_2486_cov_23.442205"
                     /isolate="AMR_MDS_5629"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1965333"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(103..1014)
                     /locus_tag="PLP58_22745"
     CDS             complement(103..1014)
                     /locus_tag="PLP58_22745"
                     /inference="COORDINATES: protein motif:HMM:NF021454.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2167 domain-containing protein"
                     /protein_id="HRJ11414.1"
                     /translation="MNIRILTLAGIIGGIPSVFTLAQEPAPSAQPSQAEMEAREKAFL
                     DRIESFGWTREGVGRLKDMAQVGIPSGWRFTDGNGTRQMLQLYQNLTSDSELGMLTTE
                     GEGPWVIFEFDDSGYVKDDEKDKLDADAMLASLQEGQRHGNERRREMGMPELHLLGWA
                     VPPRFNDQTKNLEWALRVGSQNGESINYSTRLLGRHGVMKVDLVCGPEEMDSLIPAYQ
                     NIISGYQYLGGHSYAEFREGDKMAKYGLTALVAGGAAVAAGKMGLFAKLAGFFAKLGK
                     GAIVIVIGIGVALKGLFSKLFGGRGQA"
     gene            complement(1110..2018)
                     /locus_tag="PLP58_22750"
     CDS             complement(1110..2018)
                     /locus_tag="PLP58_22750"
                     /inference="COORDINATES: protein motif:HMM:NF015431.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HRJ11415.1"
                     /translation="MQTDFLQQSNLTLDRLVDFSRVVDAGGCTQAAKAAGGAPGNFAR
                     KMKELEQCFGVTLLKPGAQGMTPTEEGMELYQIINHNLQRLGDFHRKCRGRPATVRLC
                     AGSSMLHWWVAPRLTGIVGSLNGARLDLVARRVHERVPDLRKQEADFAIVRGEAVGSQ
                     LKSCPLLVVEHLLFVSRSLLKRLRLEAGDPRLVTKLPLALPAEGDVRTILERGLERAK
                     ARVTEVIQTASFVMAARAAAAREVAAILPHIAAWELPDAEFAAVAVPALGGHTQRLHL
                     CWNPAHIRASAPAERVLPVLQHALAV"
     gene            2135..>2486
                     /locus_tag="PLP58_22755"
     CDS             2135..>2486
                     /locus_tag="PLP58_22755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ11416.1"
                     /translation="MNADLSNPWVLALLCLASSIFGYYLARVIFLYPDERRPSRRDPW
                     LETEITTSRHRTWRNAGQGVLIRREAERHRYKKRIVHDDKSCGNSHHWPPDDFHFCGG
                     VINKSERRRILKRKP"
BASE COUNT          461 a          809 c          771 g          445 t
ORIGIN      
        1 ccggtgagca gccacatgcc gccgggcagc catgccaggc actccagcag atagagcgcc
       61 gcgaagagca gccacaatgc ctggccttcg gtcatggggg gatcacgcct ggccgcggcc
      121 gccgaagagc ttgctgaaca gccccttgag cgccacaccg atgccgatga cgatgacgat
      181 ggcgcccttg cccagcttgg cgaaaaaccc ggcgagcttg gcaaaaaggc ccatttttcc
      241 cgccgccacc gccgcgcctc cggccacaag ggcggtgagg ccgtacttcg ccattttgtc
      301 gccctcgcgg aattcggcgt agctgtgccc tcccaggtat tgatagccgg agatgatgtt
      361 ttggtaggcc ggaatgagcg agtccatctc ctcggggccg cagacgaggt ccactttcat
      421 cacgccatgc ctgcccagca gccgcgtgct gtagttgatg gactcgccgt tttgcgagcc
      481 gacacgcagc gcccactcca ggttcttggt ctggtcgttg aagcgcggcg gcacggccca
      541 gccgagcaga tgcagctccg gcatgcccat ctcgcgccgg cgctcgttgc cgtggcgctg
      601 gccttcctgg agcgatgcca gcatggcgtc ggcgtcgagc ttgtccttct cgtcatcctt
      661 cacataaccg gagtcgtcga actcgaaaat gacccacgga ccctcgccct cggtggtcag
      721 catgcccagc tcgctgtcgc tggtcaggtt ttgataaagc tgcagcatct ggcgagtgcc
      781 gttgccgtcg gtgaagcgcc agccggaggg gatgcccacc tgggccatgt ccttgagccg
      841 gcccactccc tcgcgcgtcc agccgaagga ctcgatcctg tccagaaagg ctttttcccg
      901 cgcctccatc tccgcctggg aaggctgggc ggaaggagca ggctcctggg cgagggtgaa
      961 aacggagggg atgccgccaa tgatgccggc cagggtgagg atgcggatgt tcatgggaaa
     1021 aactcaggaa gctggagcct cccaggctgt cggacccgcg gcgccgggtc aactggcaaa
     1081 gcgcgtcttt ttttttgacc tgggccgggc tagactgcca gcgcgtgttg caggacaggc
     1141 agcacgcgct cagccggagc cgaggcgcgg atgtgcgcgg gattccagca cagatgcaga
     1201 cgctgagtat gaccgcccag tgcggggacg gcgacggcgg caaactccgc gtccggcagc
     1261 tcccatgcgg cgatgtgcgg caggatggcg gccacctccc gcgcggcggc ggcgcgcgcg
     1321 gccatgacga acgaagccgt ctggatgact tcggtgaccc ttgctttggc gcgctccaag
     1381 cctcgttcga gaatggtgcg cacatcaccc tccgcaggca gggccagcgg cagctttgtc
     1441 acgagacgcg gatcgccggc ttccaggcgc agccgcttga gcagcgagcg cgaaacaaaa
     1501 agcagatgct cgacgaccag gagagggcag ctcttgagct ggctgcccac cgcctctccg
     1561 cggacgatgg cgaagtcagc ctcctgcttg cgcaggtccg gcacgcgctc gtggacgcgg
     1621 cgcgccacta agtcgagacg cgcaccgttc agcgagccga cgatgccggt gaggcgcggg
     1681 gcgacccacc agtgaagcat gctgctgccc gcgcacaggc gcacggtggc gggacgtccg
     1741 cggcattttc gatgaaaatc gcccagccgc tggagattgt gattgatgat ctggtatagc
     1801 tccatgccct cctccgtggg cgtcatgccc tgggcgcccg gcttgagcag ggtcacgccg
     1861 aagcactgct ccaactcttt cattttgcgg gcgaagtttc ccggagcgcc tcccgcagcc
     1921 ttggccgcct gggtgcagcc gcccgcgtcc accacacggc tgaagtccac gagccggtct
     1981 aacgtgagat tgctctgctg caaaaagtcc gtctgcatcc agttatttca gcattcctaa
     2041 ttaaatttgc aacccccttg ccaattgcct gtcttcccaa aaacgggcgg catggaaatg
     2101 tgacggtccc gacaccaacc accccgtccc gatcatgaac gccgacctgt ccaacccctg
     2161 ggtgcttgcg ctcctgtgcc tcgcctcctc catttttggc tattatctgg cgcgcgtcat
     2221 ttttctgtat ccggacgaaa gaagaccttc ccgcagggat ccctggctcg aaaccgaaat
     2281 caccacatcc agacaccgga cttggagaaa tgccggtcag ggtgtgctga tccggagaga
     2341 ggctgaacgc cacagataca agaaacgcat cgtccacgat gacaagtcgt gcgggaattc
     2401 gcaccattgg ccgcctgatg acttccattt ttgcggcgga gtgattaaca aaagcgaacg
     2461 ccgccgcatc ctcaaaagga aacctg
//
LOCUS       DAOMYJ010000430         2482 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Prosthecobacter sp. isolate AMR_MDS_5629
            NODE_16011_length_2482_cov_13.912237, whole genome shotgun
            sequence.
ACCESSION   DAOMYJ010000430 DAOMYJ010000000
VERSION     DAOMYJ010000430.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867850
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prosthecobacter sp. (wastewater metagenome)
  ORGANISM  Prosthecobacter sp.
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Verrucomicrobiaceae; Prosthecobacter.
REFERENCE   1  (bases 1 to 2482)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2482)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.30
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:34:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,742
            CDSs (total)                      :: 4,684
            Genes (coding)                    :: 4,676
            CDSs (with protein)               :: 4,676
            Genes (RNA)                       :: 58
            tRNAs                             :: 55
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2482
                     /organism="Prosthecobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16011_length_2482_cov_13.912237"
                     /isolate="AMR_MDS_5629"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1965333"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..453
                     /locus_tag="PLP58_22760"
     CDS             <1..453
                     /locus_tag="PLP58_22760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ11417.1"
                     /translation="QQAMRDLVWLLDPGSAGARDLHARLRGLCRQMLEPAGAEVSVES
                     AGAPPERHLPLEWARDVLLFVKEALGNCARHSRARRARVGMTWSPGSFEWSLHDDGVG
                     FDEKSPAFQPGAGLRNLRKRAASLKGAAVIESAPGAGACVRLRAPLPR"
     gene            477..1127
                     /locus_tag="PLP58_22765"
     CDS             477..1127
                     /locus_tag="PLP58_22765"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HRJ11418.1"
                     /translation="MSDSSDCLRLWIIEDHRALRESLLAALLSARPAWQGRGFPGCED
                     ALSLPAGRAPDVILLDLGLPGMGGLEGLPKLKTRWPESEIVVFTVFDDSEKIYSAICA
                     GASGYLLKSESPAGVIRAVEEVRQGGSPMHPQVARKVLERLGGVRRARTGTTLSERER
                     GVLEKMVEGLTKKEISAALDLSIHTVDNYLRRIYRKLHVNTMQGAVARALKDGLVG"
     gene            1246..2460
                     /locus_tag="PLP58_22770"
     CDS             1246..2460
                     /locus_tag="PLP58_22770"
                     /EC_number="2.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007415151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004618 - phosphoglycerate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HRJ11419.1"
                     /translation="MPKKTLRDIELNGKRVLVRVDFNVPLDEQDGRMVITDDTRIRET
                     LPTLNALLEKGAKVILCSHLGRPKGKPDPRQSLAPVAPALSALLGRPVAFAADCIGDE
                     AGAKAAALQPGEVLLLENTRFHAGEEDNDRALAAGLAGLAEIFVNDAFGSAHRAHSST
                     AGVADLLPSVSGLLMEKELAWLHDELENPGRPFVVILGGAKVSDKIGVINRLLDKADS
                     IIIGGGMAYTFRKLVQGVSLGNSLYKPEWEPIAQAALDKARERGVKLLIPVDALITDK
                     LDFVAKKVGATRFTQPGENIPDGWEGVDIGPESVKIFAAEIAGARTVIWNGPMGVFEI
                     KECAQGTFDIAKAVAANSSAKTIIGGGDSVKAVKKAGLADKMTFISTGGGASLELLEG
                     KPLPGVVCLQDK"
BASE COUNT          436 a          862 c          772 g          412 t
ORIGIN      
        1 cagcaggcca tgcgcgacct cgtctggctg cttgacccgg ggagcgccgg cgcgcgcgac
       61 ctgcacgccc gcctgcgcgg cctttgccgc cagatgctgg agcctgccgg cgccgaagtc
      121 agcgtggaaa gcgctggagc gccgccggaa cgccacctgc cactggagtg ggcgcgggat
      181 gtgctgcttt ttgtcaagga agcgcttggc aactgcgccc gccacagccg cgcgcgccgg
      241 gcgcgggtgg gcatgacatg gtcccccggg tcctttgagt ggagccttca cgatgatggc
      301 gttggttttg acgaaaagtc ccccgccttt caacccggtg ccggcctgcg caacctgcgc
      361 aagcgcgccg cctcgctcaa aggcgccgcc gtcatcgaga gcgcgcccgg cgcgggcgcg
      421 tgcgttcgtt tgcgcgcccc gcttccgcgt tgagactcca ggccaccctt ttgaaaatga
      481 gcgactcttc agactgccta cgcctttgga tcatcgaaga ccaccgcgcg ctgcgcgagt
      541 ccctgctcgc cgcgctgctg tcggcgcggc ctgcctggca gggcagggga ttccctggct
      601 gcgaggacgc gctgtccctt ccggccgggc gcgcgccgga tgtcatcctc cttgatctcg
      661 gcctgcccgg catgggcggc ctggagggtc tgccaaagct caaaacacgc tggcccgaat
      721 ccgagatcgt tgtcttcacc gttttcgacg actcggaaaa aatttactcc gccatctgcg
      781 ccggcgcctc cggttatctg ctcaagtccg agagtcccgc cggagtcatc cgcgcggtcg
      841 aggaggtgcg ccagggcggc tcgcccatgc atccgcaggt ggcgcgcaag gtgctggagc
      901 gtcttggcgg agtccggcgc gcccgcaccg gcaccaccct ctccgagcgc gagcgcggag
      961 tgctggagaa aatggtcgag ggcctcacca aaaaggaaat ctccgccgcg ctcgacctca
     1021 gcatccacac ggtggacaac tacctgcgcc gcatctaccg caagctccac gtcaacacca
     1081 tgcagggagc cgtggctcga gcattgaagg acgggctggt cgggtagctt tcgagcggga
     1141 cgaggctttt ttttcatcgg cgaaaaaatt tccgcgattc ccgcctgctt tgtgctaggc
     1201 atcacgcccg cctttcccgg ccccccggat caccccctgc tcaacatgcc caaaaagacc
     1261 ctccgcgaca tcgaactgaa cggcaagcgc gtgctcgtgc gcgtggactt caacgtgcct
     1321 ctcgacgagc aggacggccg catggtcatc accgacgaca cacgcatccg cgagaccctg
     1381 ccgacgctca acgcgcttct cgaaaaaggc gccaaagtca ttctttgcag ccacctcggc
     1441 cgccccaagg gcaagccgga cccccgccag tccctggcgc cagtcgcgcc cgcgttgagc
     1501 gcgctgcttg gccgtccagt cgctttcgcc gccgactgca tcggtgacga ggccggggcc
     1561 aaagccgcag ctctccagcc gggagaagtc ctgctgctgg aaaacacccg ctttcacgcc
     1621 ggcgaggagg acaacgaccg cgccctggcc gccggcctcg ccggactggc cgagattttt
     1681 gtcaacgacg cattcggctc cgcgcaccgc gcccacagct ccaccgcagg cgtggcggat
     1741 ctgctgccgt ccgtctcggg cctgctcatg gagaaggagc tggcctggct gcacgacgag
     1801 ctggaaaacc ccgggcgccc cttcgtggtc atcctcggcg gcgccaaggt gagcgacaag
     1861 atcggagtca tcaaccgcct gctcgacaag gccgacagca tcatcatcgg cggtggcatg
     1921 gcctacacct tccgcaagct cgtccagggc gtcagtctgg gaaacagcct ctacaaaccg
     1981 gagtgggaac ccatcgccca ggccgcgctc gacaaggcca gggagcgcgg cgtgaagctg
     2041 ctcatcccgg tggacgcctt gatcactgac aagctcgatt ttgtcgccaa aaaggtgggc
     2101 gccacccgct tcacccagcc gggtgaaaac atcccggacg gctgggaggg cgtggacatc
     2161 gggccggagt ctgtgaagat cttcgccgcc gagatcgccg gtgccaggac cgtcatttgg
     2221 aacggaccga tgggtgtttt tgagatcaag gaatgcgctc aaggcacctt cgacattgcc
     2281 aaagccgtgg ccgccaacag ctccgccaag accatcatcg gcggcggtga cagcgtcaaa
     2341 gccgtcaaga aagccggact ggccgacaag atgaccttca tttccaccgg tggcggagcc
     2401 agcctcgaac ttcttgaagg caagcccctg cccggcgtcg tttgtctcca ggacaagtga
     2461 gcctttcccc gctgacggac tt
//
LOCUS       DAOMYJ010000431         2474 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Prosthecobacter sp. isolate AMR_MDS_5629
            NODE_16105_length_2474_cov_18.000827, whole genome shotgun
            sequence.
ACCESSION   DAOMYJ010000431 DAOMYJ010000000
VERSION     DAOMYJ010000431.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867850
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prosthecobacter sp. (wastewater metagenome)
  ORGANISM  Prosthecobacter sp.
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Verrucomicrobiaceae; Prosthecobacter.
REFERENCE   1  (bases 1 to 2474)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2474)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.30
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:34:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,742
            CDSs (total)                      :: 4,684
            Genes (coding)                    :: 4,676
            CDSs (with protein)               :: 4,676
            Genes (RNA)                       :: 58
            tRNAs                             :: 55
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2474
                     /organism="Prosthecobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16105_length_2474_cov_18.000827"
                     /isolate="AMR_MDS_5629"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1965333"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1124)
                     /locus_tag="PLP58_22775"
     CDS             complement(<1..1124)
                     /locus_tag="PLP58_22775"
                     /inference="COORDINATES: protein motif:HMM:NF027806.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HRJ11420.1"
                     /translation="MIVSNPWFLAALAGVLVVFHLEWLAALLNSARIGKALPPVLADL
                     YTDEKRGQLREYFLDKQRLGLTQSFTSLALLLGVWWIGGFGWLQSACASLGHGPVLTG
                     LIVITAVMLALTLAGLPFEIWHTFGVEAKHGFNKTTPRTFVSDQLKGLLLMALLGLPA
                     AALVLWFFETQALAALYAWLFLAAFSLLMTWLSPRLIMPLFLKFRPLEEGALRAGILA
                     LAEKLHFPVGEVSIVDGSRRSTKANAFFAGFGKTRRIALFDTLLENHTQEEILAVLAH
                     EIGHSRLRHVPKHLALALAEMGVIFALLGWALRSPDFFAAFGVAGTPVGMGLVLFGLI
                     YKPLGLLIGIGGLALSRRHEFEADAFAARAVGGPEPLLRS"
     gene            complement(1121..2110)
                     /gene="queG"
                     /locus_tag="PLP58_22780"
     CDS             complement(1121..2110)
                     /gene="queG"
                     /locus_tag="PLP58_22780"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018969730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0052693 - epoxyqueuosine reductase
                     activity [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HRJ11421.1"
                     /translation="MAPARPAAHRELYEKWIADGKHGDMAWMARNLDRRADPRLVLPG
                     ARSIIVLAMNYYQGAAPGPAAADYRIARYAWNDDYHDLIEKKLRAFHDHLVTLGGAQR
                     YYVDTGPVLERDFANEAGLGWNGKSTMQIHRQLGTWFFLAELITTLDLAPDEPARDLC
                     GKCTRCMDACPTQAITAPRSLDARRCVSYLTIESKGPIPEEFRRAIGDRIYGCDDCLT
                     ACPWNKFAQASREAAFQAREAIFQHRLRDFLSLTDDSFRALFAKSPIKRIKRPAFLRN
                     VCVALGNTGTPADLPALAAAARDEHPLISGHAHWAIAEIQRRFLVSSTPASHP"
     gene            2045..2293
                     /locus_tag="PLP58_22785"
     CDS             2045..2293
                     /locus_tag="PLP58_22785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ11422.1"
                     /translation="MFAVGDPFFIKLAVRGGARGGDAAVGKAEVAGEGAEGGWFWVLS
                     LESSVLGLGSWIFDLGFCILHSAFCILNFALLRILHFD"
BASE COUNT          421 a          644 c          972 g          437 t
ORIGIN      
        1 tccgatctga gcaggggctc cggcccgccg acggcgcgcg cggcgaaggc gtcggcctcg
       61 aactcatgcc gccggctcag cgccaggccg ccgatgccga tgagcaggcc gagcggtttg
      121 tagatgaggc cgaaaagcac caggcccatg cccacgggtg tgcccgccac gccgaaggcg
      181 gcgaagaaat ccggcgagcg cagcgcccac ccgagcaggg cgaagatgac gcccatctcc
      241 gccagggcca gggcgaggtg cttcggcaca tggcgcaggc ggctgtggcc gatctcatgc
      301 gcgagcacgg cgaggatttc ctcctgcgtg tggttttcca gcagggtgtc gaagagggcg
      361 atgcggcgcg tcttcccgaa gccggcgaag aaggcgttgg ccttcgtgga gcggcgggag
      421 ccgtccacga tgctgacctc gccgacgggg aagtggagct tttcggcgag ggcgaggatg
      481 ccggcgcgca gggcgccctc ctccaggggg cggaacttta aaaacagggg catgatgagc
      541 cggggggaga gccaggtcat gagtaggctg aaggcggcga ggaagagcca ggcgtaaagc
      601 gcggccaggg cctgcgtctc gaaaaaccag agcacgagcg ctgcggcggg caggccgagc
      661 agggccatga gcagcaggcc tttgagctgg tcgctgacga aggtgcgcgg ggtggttttg
      721 ttgaagccgt gcttggcctc gacgccgaag gtgtgccaga tctcaaacgg cagcccggcc
      781 agggtgagcg cgagcatgac ggcggtgatg acgatgaggc cggtgagcac gggcccgtgc
      841 ccgaggctgg cgcaggcgct ttgcagccag ccgaagccgc cgatccacca aacgccgagc
      901 agcagcgcca gggaggtgaa gctctgggtg aggccgaggc gctgcttgtc gaggaagtat
      961 tcgcggagct gcccgcgctt ttcgtcggtg tagaggtccg ccagcacggg tggcagggct
     1021 ttgccgatgc gggcgctgtt cagcagggcg gccagccatt cgagatggaa gacgacgagc
     1081 actcccgcga gcgcggcgag gaaccagggg ttggagacaa tcatgggtgg gaagctgggg
     1141 tggatgaaac gaggaaccgg cgctgaatct cggcgatggc ccagtgggcg tgcccggaga
     1201 tgagggggtg ctcgtcgcgg gcggcggcgg cgagggcggg gaggtcggcg ggggtgccgg
     1261 tgttgccgag ggcgacgcag acgttgcgca ggaaggcggg gcgcttgatg cgcttgatgg
     1321 gggatttggc gaagagggcg cggaagctgt cgtcggtgag gctgaggaag tcgcgcaggc
     1381 ggtgctggaa gatggcttcg cgcgcctgga aggcggcttc gcgggaggct tgggcgaatt
     1441 tgttccaggg gcaggcggtg aggcagtcgt cgcagccata gatgcggtcg ccgatggcgc
     1501 ggcggaactc ttcggggatg ggccctttgc tctcgatggt gaggtaggag acgcagcggc
     1561 gggcatcgag gctgcgcggg gcggtgatgg cctgggtggg gcaggcatcc atgcagcggg
     1621 tgcatttgcc gcagaggtcg cgcgcgggct cgtcgggagc gaggtcgagg gtggtgatca
     1681 gctcggcgag gaagaaccag gtgccgagct ggcggtggat ctgcatggtg cttttgccat
     1741 tccagccgag gccggcctcg ttggcgaagt cgcgctccag cacggggccg gtgtccacat
     1801 aatagcgctg cgcgccgccg agggtgacga ggtggtcatg gaaggcgcgg agcttttttt
     1861 cgatgaggtc gtggtagtcg tcattccatg cgtagcgggc gatgcggtaa tccgctgcgg
     1921 cggggccggg ggccgcgccc tggtagtagt tcatggcgag cacgatgatg ctgcgtgcgc
     1981 cgggcaggac gaggcgtgga tcggcgcggc ggtcgaggtt gcgcgccatc caggccatgt
     2041 cgccgtgttt gccgtcggcg atccattttt cataaagctc gcggtgcgcg gcggggcgcg
     2101 cgggggcgat gcggcagtcg gcaaagccga ggtcgcgggc gagggcgcgg agggcggctg
     2161 gttttgggtc ttgagtcttg agtcttcggt cttgggtcta ggatcttgga tttttgatct
     2221 tggattttgc attttgcact ctgcattttg cattttgaat tttgcattgc tccgcatttt
     2281 gcatttcgac tgaccactga caactgttca ctctgttcat tgttcgttta cgcgtgttgc
     2341 gctactatta caaggttatg ttggacgctg gattggttta cacaaaagaa acggaacagg
     2401 cgacaagact gccgtttcca tataacaagc aggatttctg catttacgag cccatcgaga
     2461 aaaagatcga ttac
//
LOCUS       DAOMYJ010000432         2467 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Prosthecobacter sp. isolate AMR_MDS_5629
            NODE_16172_length_2467_cov_18.690299, whole genome shotgun
            sequence.
ACCESSION   DAOMYJ010000432 DAOMYJ010000000
VERSION     DAOMYJ010000432.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867850
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prosthecobacter sp. (wastewater metagenome)
  ORGANISM  Prosthecobacter sp.
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Verrucomicrobiaceae; Prosthecobacter.
REFERENCE   1  (bases 1 to 2467)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2467)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.30
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:34:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,742
            CDSs (total)                      :: 4,684
            Genes (coding)                    :: 4,676
            CDSs (with protein)               :: 4,676
            Genes (RNA)                       :: 58
            tRNAs                             :: 55
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2467
                     /organism="Prosthecobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16172_length_2467_cov_18.690299"
                     /isolate="AMR_MDS_5629"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1965333"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            217..570
                     /locus_tag="PLP58_22790"
     CDS             217..570
                     /locus_tag="PLP58_22790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ11423.1"
                     /translation="MSDSFQNQEEQRLQRQKLEAEKKRHFIATRDLDRDGDVDLADDL
                     SHDMNRDGRINANDRDHYDLDGDNEIDAADRTLAAAQERQGESVGQALGMDKTSEGWQ
                     RAQTQQIKPQGQGVR"
     gene            688..1347
                     /gene="trmD"
                     /locus_tag="PLP58_22795"
     CDS             688..1347
                     /gene="trmD"
                     /locus_tag="PLP58_22795"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009959182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052906 - tRNA
                     (guanine(37)-N(1))-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HRJ11424.1"
                     /translation="MITLFPELVSVPMGTSIMGRARERGIITLGVHDLREHGLGRHRQ
                     VDDTPCGGGPGMVLRPEPLFAALEALDTERARVILMTPGGRRFDQAAARRLAAEEHLI
                     FISGHYEGVDERVVEHWVDEELSIGDFVLTNGAIAAVVVMDALVRLLPGVLGDDTSAV
                     DESFGASGLLEAPHYTKPAEFRGMRVPEVLLGGNHAKIAQWRAAQSLERTRRNRPDLL
                     A"
     gene            1344..>2467
                     /locus_tag="PLP58_22800"
     CDS             1344..>2467
                     /locus_tag="PLP58_22800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ11425.1"
                     /translation="MSADTKTQAQVLARWRGMGALLAVAFYSLFFAAHRQLQETPAHV
                     RDAVIFEARSQSVFLALTEPRERDKRGIGAEHPAFVLLHQPPVQLLMKGWLALGLDLN
                     SARKHGVAMLTGAAGALSVVMVYHALLWSGIGRMRALMLAAVHGAGTGTLMMAALPEV
                     WIFAGMSVAALAAVTARGALAPWWLHLAAAAYAVSCFIGSVIAVVLFGLVRCAQDTAL
                     NRRFTPGPLLVTLAALTLAFGLANTQRAVFPLSPPLPEDLLSWRVRDAGWPAGESLPG
                     RVTREIFLTNLVAPPMPVTRSDPGGTRSARRIVVLDDADWSRMDWQKGVGGGWLLLLA
                     LACAGLCWRARLDPYALGILAALAWTVAALAWHGSPGRLLL"
BASE COUNT          380 a          798 c          885 g          404 t
ORIGIN      
        1 ccgctgccgc gccagcggct cccgcggcag gcgtcgaggc ccctgcggcc gcacagtgga
       61 aacaaggctc tgtgggcgaa gccttgaaaa aagaaggcta ccctgtgcgc aggcaaggcg
      121 gaccgcgcat cagccaccag cgccgcgagc aaaagatcgg cacccaccgt cgtaaataag
      181 gctcatttcc ccctttgttt cggcaccccc gtcaccatga gcgactcctt ccaaaaccag
      241 gaagaacagc gtcttcaaag gcagaagctg gaggcggaaa agaaacggca tttcatcgcc
      301 accagggacc tggaccgcga cggggatgtg gacctcgccg acgatctctc ccacgacatg
      361 aaccgcgacg ggcgcataaa cgccaatgac cgcgaccatt acgaccttga cggcgacaac
      421 gagatagacg ccgccgaccg caccctggcc gccgcgcagg agcggcaggg agaaagtgtg
      481 ggccaggcgc tcggcatgga caaaacctcc gagggctggc agcgcgcgca gacacagcag
      541 atcaagccgc aggggcaggg ggtgaggtga gcgcggttcg cgtgacgcgg acactcctgt
      601 ccgcccgcgt tggggcggat cgaaattcgc atttcggctt ttgaaattcc cccgcaaccg
      661 ccctagagca gcgaagtgcg tctggatgtg atcacgcttt tccccgaact tgtctcggtg
      721 ccgatgggca cgagcatcat gggccgcgcc cgggagcgcg gcatcatcac gctcggcgtg
      781 catgacctgc gcgagcacgg cctgggcagg caccgccagg tggatgacac gccctgcggc
      841 ggcggtccgg gcatggtgct gcggccggag ccgctgttcg cggcgctgga ggccttggac
      901 accgaacgcg cgcgggtgat cctgatgacg ccgggggggc ggcgctttga ccaggcggcg
      961 gccaggcggc tggcggcgga ggagcatttg attttcatct ccggccacta cgagggcgtg
     1021 gacgagcgcg tggtggagca ttgggtggac gaggagctga gcatcgggga ctttgtgctg
     1081 accaatggcg ccatcgccgc cgtggtggtc atggacgccc tcgtgcgcct gctgcccggc
     1141 gtgttggggg atgatacgag cgcggtggat gagtcctttg gcgcttcggg gctgctggag
     1201 gcgccgcatt acacgaagcc ggcggagttt cgcggcatga gggtgccaga agtgctgctt
     1261 ggtggaaatc acgcgaagat tgcgcaatgg cgcgccgccc agtccctgga gcgcacgcgg
     1321 cggaaccgcc cggatttgct ggcatgagcg cggacacgaa aacacaggca caggtgctcg
     1381 cacgctggcg cggcatgggc gcgctgctgg cggtggcttt ttacagcctg ttcttcgccg
     1441 cccaccgcca gctccaggaa acgcccgcgc atgtgcggga cgcggtcatc ttcgaggcgc
     1501 gctcgcagtc cgtctttctt gcccttaccg agccgcgcga gcgcgacaag cgcggcatcg
     1561 gcgcggagca tcccgcattc gttctcctgc accagccgcc ggtgcaactg ctgatgaaag
     1621 gctggctggc gctcggcctg gatctcaaca gcgcgcgcaa gcacggcgtg gccatgctca
     1681 ccggcgcggc cggcgcgcta agcgtggtga tggtctatca tgcgctgctg tggagcggga
     1741 tcgggcggat gcgcgcgctg atgctggcgg cggttcacgg cgcgggcacg ggcacgctca
     1801 tgatggcggc gctgccggag gtgtggatct tcgccggcat gtccgttgcc gcgctcgcag
     1861 cggtgacggc gcgcggggcg ctggcgccgt ggtggctgca tctggcggcg gcggcttacg
     1921 cggtgtcatg cttcataggc agcgtcatcg ccgtggtcct gttcggcctg gtccgctgcg
     1981 cgcaggacac ggcattgaac cgccgcttca ctcccgggcc gctgcttgtc actcttgccg
     2041 cgctgacgct ggcgttcgga ttggcgaaca cccagcgcgc ggtctttcct ctgtccccgc
     2101 cgctgccgga ggatctgctg agctggcgag tgcgggacgc cggctggccc gccggggaaa
     2161 gcctgcccgg ccgcgtgacg cgggagattt ttctgaccaa tctcgtggct ccgccgatgc
     2221 ccgtgacgcg gtccgacccc ggcggcactc ggagcgcgcg tcgcatcgtc gttctcgacg
     2281 atgccgactg gtcccgcatg gactggcaga aaggcgtggg cggtggctgg ctgctgctgc
     2341 tggccctggc ctgcgcgggg ctgtgctggc gcgcgcggct ggatccctac gcgctgggca
     2401 ttctcgccgc gctggcatgg accgtggccg cccttgcctg gcacggctcg ccaggccgcc
     2461 tgctgct
//
LOCUS       DAOMYJ010000433         2459 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Prosthecobacter sp. isolate AMR_MDS_5629
            NODE_16236_length_2459_cov_19.996256, whole genome shotgun
            sequence.
ACCESSION   DAOMYJ010000433 DAOMYJ010000000
VERSION     DAOMYJ010000433.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867850
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prosthecobacter sp. (wastewater metagenome)
  ORGANISM  Prosthecobacter sp.
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Verrucomicrobiaceae; Prosthecobacter.
REFERENCE   1  (bases 1 to 2459)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2459)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.30
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:34:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,742
            CDSs (total)                      :: 4,684
            Genes (coding)                    :: 4,676
            CDSs (with protein)               :: 4,676
            Genes (RNA)                       :: 58
            tRNAs                             :: 55
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2459
                     /organism="Prosthecobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16236_length_2459_cov_19.996256"
                     /isolate="AMR_MDS_5629"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1965333"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(122..715)
                     /locus_tag="PLP58_22805"
     CDS             complement(122..715)
                     /locus_tag="PLP58_22805"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HRJ11426.1"
                     /translation="MPAVFDLQDCLRRARLGDTEAGGALVAHLHPLVARLVRRHLPRR
                     ELEEDLAQEVFIKILQRLDRYEERQGVPFEHWVSRLTVRTCLDALRAEKARPEWRVAD
                     LGEGGSEWVDFLLERQPTPPVERATDAAEVLERLLCRLSPPDRLVITLLDLEERSTAE
                     ISQMTGWTRPMVKMRAMRARRKLRGIAREIMKEEFDP"
     gene            877..2142
                     /gene="bioA"
                     /locus_tag="PLP58_22810"
     CDS             877..2142
                     /gene="bioA"
                     /locus_tag="PLP58_22810"
                     /EC_number="2.6.1.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009959923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004015 -
                     adenosylmethionine-8-amino-7-oxononanoate transaminase
                     activity [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine--8-amino-7-oxononanoate
                     transaminase"
                     /protein_id="HRJ11427.1"
                     /translation="MLPQLLALEKRHLWHPFTPMREWCAPEHEPLLLAEGKGCVLRDQ
                     HGREYLDGNSSIWTNIHGHGHPVIHAAIKEQLDRVAHTSFLGFTHEPAIRLAGELVDF
                     FPEGTLTRVFYSDDGSTAVECALRMALQFWRQNGCPERDTILAFDHAYHGDTLGAAST
                     GGIPLFKGSGNDFGYRTLRVPDLDRLDALTRDETRRLAAVILEPLIQGSAGMRLWPRG
                     MLAALRQWCDRHDVLLILDEVMTGFGRTGMMFACELEGVTPDLICLAKGLTGGLLPMA
                     ATLTTERVFEGFLGREKTFYYGHSYTASQLGCAAALASLRVFREERVLESLPGKTACF
                     TSLLDGLRALPRVKEIRQCGMIAGIEIGPFDTALLTGAAVCLAARKHGLLTRPILDTL
                     VLMPPLCVTSSELERMTRALKLALEEVCG"
     gene            complement(2178..>2459)
                     /locus_tag="PLP58_22815"
     CDS             complement(2178..>2459)
                     /locus_tag="PLP58_22815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ11428.1"
                     /translation="AAGVKDGVTTRKDIIRKLGLFYTKGTAASGQAAATWKFQTTAST
                     AKSWIPGSAFIPGALITYDQSLTVVLDANDVVVSHSFLETSREKTGLGY"
BASE COUNT          422 a          844 c          776 g          417 t
ORIGIN      
        1 gcgcgcgcga gcacccgggt ggcaaagccg aacggcatct gcggcagggg cgtctgcgcc
       61 cggcgcgcgt cagcggccag cgcctgccag cgcggttcga agtccctcat cggtcgtgtg
      121 ttcatgggtc aaactcctcc ttcatgattt cgcgcgcgat gccgcgcagc ttccgccgcg
      181 cgcgcatggc gcgcattttc accatcgggc gcgtccagcc cgtcatctgg ctgatctccg
      241 ccgtggaacg ctcctccagg tccagaagcg tgatcaccag ccggtccggc ggggacaggc
      301 ggcaaagcag gcgctccagc acctccgccg cgtccgtggc gcgctccacc ggcggcgtgg
      361 gctggcgttc gagcaggaaa tccacccact ccgagcctcc ctcccccaaa tcggccacgc
      421 gccactccgg gcgcgccttt tccgcgcgca gcgcgtccag gcaggtgcgc accgtgaggc
      481 gcgacaccca gtgctcgaaa ggcacgccct ggcgctcctc gtagcggtcc agccgctgga
      541 ggattttgat gaagacctcc tgcgccagat cctcctccaa ctcccgccgc ggcaggtgcc
      601 gccgcaccag cctggccacc agcggatgca gatgcgcgac gagcgcgccg cccgcctccg
      661 tgtctccaag gcgcgcgcgc cgcaggcagt cttgcagatc gaaaacagcc ggcatcacgg
      721 ttcaaagagg cattgtggaa ggcgggacac accgcagcag caaacgcacc ccgccgccgc
      781 agggtaacaa ccgcgtcaaa aaattgaaag cgggaatcca ccctcagggg tcgaatcgcg
      841 aagttgcatc cgtcgcgggc gcgtcctcat tcaggcgtgc tgccgcaact gctcgccctc
      901 gaaaaacgcc atctctggca tcctttcacg ccgatgcggg aatggtgcgc gccggagcat
      961 gagccgctgc tgcttgcgga gggaaagggc tgcgtgctgc gcgaccagca tggccgcgag
     1021 tacctcgacg gcaacagctc catctggacc aacatccacg gccacggcca cccggtcatc
     1081 catgccgcca tcaaggagca gctcgaccgc gtggcgcaca cctccttcct cggtttcacc
     1141 cacgagcccg ccatccggct ggccggggag ctggtggatt ttttccccga aggcacgctc
     1201 acccgcgtct tttactccga cgacggcagc acggccgtgg agtgcgcgct gcgcatggcc
     1261 ctgcagttct ggaggcaaaa tggatgcccg gagcgcgaca cgatccttgc ctttgaccac
     1321 gcctatcacg gtgacacact cggcgcggcc agcacgggag gcatcccgct attcaaaggc
     1381 agcggcaacg acttcggcta ccgcaccctg cgtgtcccgg acctggaccg gctcgacgct
     1441 ttgacgcggg atgagacccg ccgcctggcc gccgtgatcc tcgagccgct catccagggc
     1501 agcgccggca tgaggctctg gccgcgcggc atgctcgccg ccctgcgcca atggtgcgac
     1561 cggcacgacg tgcttctcat ccttgacgag gtgatgaccg gctttggccg caccgggatg
     1621 atgttcgcct gcgagctcga gggagtgacg ccggatttga tttgcctggc caaggggctc
     1681 acaggcggct tgctgccaat ggcagccaca ctgaccacgg agcgtgtgtt cgaagggttc
     1741 cttggccgcg aaaaaacctt ttattacggc cacagctaca ccgccagcca gctcggctgc
     1801 gcggccgcgc ttgccagcct gcgggttttt cgcgaggagc gtgtgctgga gtctctgccg
     1861 ggaaagacag cgtgtttcac cagcctgctc gacgggttgc gcgccctgcc ccgggtcaaa
     1921 gaaatccgcc agtgcggcat gatcgcgggc atcgaaatcg gtcccttcga caccgcgctg
     1981 ctcactggcg ccgccgtctg cctggccgcg cgaaaacacg gcctgctcac acgccccatt
     2041 ctcgacaccc tggtgctcat gccgccgttg tgtgtcacca gctccgaatt ggagcgcatg
     2101 acacgcgcct tgaagctggc gctggaggaa gtctgcggat gagttttttt gaaaccgcgg
     2161 tccgccgtgc cctcgcgtta atagccaagt ccggtttttt cacggctggt ttccaggaag
     2221 gagtgcgaaa ccacgacatc attggcatcc agcaccacgg tcaggctttg atcataagtg
     2281 atgagcgcgc cgggaatgaa ggcggaaccc ggaatccagg acttggcggt ggaggcggtg
     2341 gtctgaaact tccaagtggc agcggcctgg ccggaggcgg cggtcccttt ggtgtaaaag
     2401 agaccgagct tgcggatgat gtccttgcgt gtggtgacac cgtctttcac gccggcggc
//
LOCUS       DAOMYJ010000434         2399 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Prosthecobacter sp. isolate AMR_MDS_5629
            NODE_16852_length_2399_cov_14.840870, whole genome shotgun
            sequence.
ACCESSION   DAOMYJ010000434 DAOMYJ010000000
VERSION     DAOMYJ010000434.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867850
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prosthecobacter sp. (wastewater metagenome)
  ORGANISM  Prosthecobacter sp.
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Verrucomicrobiaceae; Prosthecobacter.
REFERENCE   1  (bases 1 to 2399)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2399)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.30
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:34:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,742
            CDSs (total)                      :: 4,684
            Genes (coding)                    :: 4,676
            CDSs (with protein)               :: 4,676
            Genes (RNA)                       :: 58
            tRNAs                             :: 55
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2399
                     /organism="Prosthecobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16852_length_2399_cov_14.840870"
                     /isolate="AMR_MDS_5629"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1965333"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            168..656
                     /locus_tag="PLP58_22820"
     CDS             168..656
                     /locus_tag="PLP58_22820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ11429.1"
                     /translation="MKQGARHQWALVGDASVADQGGLVIAPGTGVMFKARAPAAFTLT
                     GHVRQNSFLRALNEGHNLLSPPWPLAATPRRLHFTAANAFTAAATADAADQLQFWKGD
                     TAPGAEGYDIHWLQNTGAQGAWISPEGADVSESLVLPAHRAFFLRARPATAAQGWWCP
                     AP"
     gene            complement(942..1652)
                     /locus_tag="PLP58_22825"
     CDS             complement(942..1652)
                     /locus_tag="PLP58_22825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ11430.1"
                     /translation="MNIDKLSLNDALRYFFCGAAFMLAFARGADWDASDFKTLTGASV
                     AGIGLIALTSGAIIYSIYRALIYVYTSRIAGCLSSMTTSWNERKISYWLPTHVPSAEF
                     DEDIWRWTFGAENGKQDEAARGRLSEWGSQVHLCYTIALAIGMGSFFGVLAIGKCPSP
                     DRFLILIAVFFLSPASFLTFASRALNPVSANQNHQNRVPVTDLIKLSSFKASSGQGRK
                     PLPAAGCRSPGWGMPEDS"
     gene            complement(1843..>2399)
                     /locus_tag="PLP58_22830"
     CDS             complement(1843..>2399)
                     /locus_tag="PLP58_22830"
                     /inference="COORDINATES: protein motif:HMM:NF014724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ElyC/SanA/YdcF family protein"
                     /protein_id="HRJ11431.1"
                     /translation="GGAHCHAETSGVPKTGAALVLGCSPRLADGRRNLFFTKRIQAAA
                     ELYHSGRVRALIVSGDNSTKHYDEPTAMKEALIQAGVPAAAIHCDYAGFRTLDSVVRA
                     HAVFGQREFIIVSQRFHNERAVFIARRHGLEAHGFDAPDVSRRIAPTTYLREYLARVK
                     AVLDVTLLRTKPKFYGPPVEIGLE"
BASE COUNT          423 a          754 c          782 g          440 t
ORIGIN      
        1 cgagactcac ttgaagcccg ccttgcccgc gcactggctg gaactcccag ccgcatggca
       61 cgctgggcca ggtctttgac aagcacctgc tcaccggcgg ccccgcctcg gcgcgcgcgg
      121 accaggtcat cttccacgac ggctccggct ggcggactta ctggctcttg aagcagggcg
      181 ctcggcatca gtgggcgctc gtcggcgacg cctccgtggc ggatcaaggc ggcctcgtca
      241 tcgcccccgg caccggcgtc atgttcaagg cgcgcgcccc cgccgccttc accctcaccg
      301 gtcatgtgcg gcaaaactcc ttcctgcgcg cgctgaacga gggccacaac ctgctctccc
      361 cgccctggcc gctcgccgcc accccgcgcc gcctgcattt caccgccgcc aacgccttca
      421 ctgcggcggc cactgcggat gccgccgacc aactccagtt ctggaaaggc gacaccgccc
      481 ccggcgccga aggctacgac atccactggc tgcaaaacac cggtgcacaa ggcgcgtgga
      541 tttcaccgga aggcgccgat gtcagcgaaa gcctcgtcct ccccgcccac cgcgccttct
      601 tcctgcgcgc ccgccccgcc accgccgccc agggctggtg gtgccccgcg ccgtgaggtg
      661 ggcatgtagg cgcattcgcc agaatgcggg agatgcgggt gttagcggga gtcaaacgcc
      721 gcgctttggc aagcgcgcct acggcgccag gtagggcggg ctgtcctcag cccgccgcgc
      781 gcggaggctg cgggtccgca tggcgcgtac gggcagctcg gaggtacgcc tcccgcacgc
      841 ggcgcttcga ggacgaagcg ccctacctcg gagccgccca gggctggtgg tgcccggtgc
      901 cgtgaggcgg cctctgcata gtgcaaccgt cctggttaca ctcaagagtc ttcaggcatg
      961 ccccacccag gactgcggca gcctgctgca gggagtggct tccttccctg ccctgaagat
     1021 gccttgaaac tggacagttt tatcaagtca gtcacaggaa cccgattctg atggttttgg
     1081 tttgccgaga ctggattcaa agctcttgag gcgaaggtga gaaaagaagc cggcgataaa
     1141 aaaaacaccg caataagaat gagaaaacgg tcagggctcg gacactttcc aatggcgaga
     1201 acaccaaaga aggagcccat cccgattgcg agggcaattg tatagcagag gtgaacctgg
     1261 gagccccact cagacaatct gcctctcgct gcctcatcct gctttccgtt ttcggctcca
     1321 aatgtccaac gccatatgtc ttcgtcgaac tctgcggatg ggacatgggt aggcagccag
     1381 tagctgatct ttctctcgtt ccagctggtg gtcatactgc taagacagcc agcgatccga
     1441 ctcgtgtaga catagatcaa cgctctgtaa atagagtaga ttatcgctcc tgatgtaaga
     1501 gcaatgagcc cgatcccggc aactgacgct ccggtgagcg tcttgaaatc tgatgcatcc
     1561 cagtcggcac ccctcgcgaa ggccaacatg aatgccgctc cacaaaagaa gtatcgaagt
     1621 gcatcattca ggctgagttt atcgatgttc atttttttcg ctgaacagtc ttattcgtgc
     1681 ggtgcgagat cggttgtggc gatattccgg ggcgacgatg cgcggcgagg ctcggatggc
     1741 aaggtttttg gaagtgcgct tcgggtttgg agtggttcga acgccgtgcg ttgggcaggg
     1801 tggcggcgtg ccgggagggg gatggtgtct gaggatgctt ttttactcca gcccgatctc
     1861 gacgggcggg ccgtagaatt tcggttttgt gcgcagaagg gtcacgtcca ggacggcttt
     1921 cacgcgggcg aggtattcgc gcaggtaggt ggtgggggcg atgcggcggg agacatccgg
     1981 cgcgtcaaag ccgtgcgcct ccagcccgtg gcggcgggcg atgaagacgg cgcgctcgtt
     2041 gtggaagcgc tgggagacga tgatgaactc gcgctgcccg aagacggcat gggcgcgcac
     2101 cacggagtcg agcgtgcgga agccggcgta gtcgcagtgg atggccgccg cgggcacgcc
     2161 ggcctggatg agcgcctcct tcatggcggt gggctcgtcg tagtgtttgg tgctgttgtc
     2221 gccgctgacg atgagggcgc gcaccctgcc ggagtggtag agctccgccg cagcctggat
     2281 gcgcttggtg aaaaacagat tgcgccgccc gtccgccagc cggggcgagc agccgagcac
     2341 cagggccgcg cccgtctttg gcacgccgct ggtttcggcg tggcagtgcg cgccgccgg
//
LOCUS       DAOMYJ010000435         2378 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Prosthecobacter sp. isolate AMR_MDS_5629
            NODE_17075_length_2378_cov_23.139905, whole genome shotgun
            sequence.
ACCESSION   DAOMYJ010000435 DAOMYJ010000000
VERSION     DAOMYJ010000435.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867850
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prosthecobacter sp. (wastewater metagenome)
  ORGANISM  Prosthecobacter sp.
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Verrucomicrobiaceae; Prosthecobacter.
REFERENCE   1  (bases 1 to 2378)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2378)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.30
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:34:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,742
            CDSs (total)                      :: 4,684
            Genes (coding)                    :: 4,676
            CDSs (with protein)               :: 4,676
            Genes (RNA)                       :: 58
            tRNAs                             :: 55
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2378
                     /organism="Prosthecobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17075_length_2378_cov_23.139905"
                     /isolate="AMR_MDS_5629"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1965333"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            20..778
                     /locus_tag="PLP58_22835"
     CDS             20..778
                     /locus_tag="PLP58_22835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009962467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0046855 - inositol phosphate
                     dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HRJ11432.1"
                     /translation="MNDLLPLAIEAATQAGRLIKDNFKSEKTVNEMKRRDVKLELDVR
                     TQKLITGILLAAHPEHAILGEEEGDLGGEGPVEWIVDPIDGTVNYFYGIPHFCVSIAA
                     RMRESGEYLLGVIHDPMQGETWTVEKGGPPRLDGQAVRVSPRENMSEAIITAGFSKSK
                     SALDAGFERYRRISYEVFKTRMLGSAALALAYVATGRLDAYVEEQISLWDIAAGVQLV
                     EAAGGRVILRPSTVKEDTFFICAWNGRLPIEQYL"
     gene            complement(785..2113)
                     /locus_tag="PLP58_22840"
     CDS             complement(785..2113)
                     /locus_tag="PLP58_22840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009958988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HRJ11433.1"
                     /translation="MSQTITTTRRQFLTRAAGGLFAAPFVTTGMRAASPNGKLRHAAF
                     GASGMSWADISQMANHEMWELTAVADVDTRNFARIKEKFPHARCYQDWRELLEKEAGN
                     IDSVNVSTPDHMHGPMGMRVLELGKHMYGQKPLAQNLFECRQLMLKAREKKVMTQMGI
                     QISSAFTERHAVAIIQQGTIGKVKEVHTFSNKFWGDMDPVPQRADAVPEGFDWQLWLG
                     PAADRPYMDKYYHPGQWRKRRDFGTGTLGDMGCHMFSGWFRALSLAAPVGVKSTGPAP
                     LNLTNWAVNAVVEYTFKGTEFTAADTVKVTWYDGDARPPKEVMALADPEKFPGQGSLY
                     IGTDGVLLAPHGSTPMLYPRGKFTGFQYPKLEPRDHWRDYIDCCLKGDKQPSANFDYA
                     GPMTEAVLLGCLASAFPGQDLVWDEPGLKIPNNEAANALVRRKYRAGWEL"
     gene            2204..>2378
                     /locus_tag="PLP58_22845"
     CDS             2204..>2378
                     /locus_tag="PLP58_22845"
                     /inference="COORDINATES: protein motif:HMM:NF012242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp70 family protein"
                     /protein_id="HRJ11434.1"
                     /translation="MSLILGIDLGTTHSLVGVMDSGFPILLADAEGGRLTPSAVHFGA
                     DGSVTVGAPALRRR"
BASE COUNT          394 a          813 c          741 g          430 t
ORIGIN      
        1 agacgccccc atcccctcag tgaacgacct cctccccctc gccatcgaag ccgccaccca
       61 ggcgggccgc ctcatcaagg ataacttcaa gtccgagaaa accgtcaacg agatgaagcg
      121 ccgcgatgtg aagctggagc tcgatgtgcg cacccagaag ctcatcaccg gcatcctgct
      181 cgccgcccac ccggagcacg ccatcctcgg cgaggaggag ggcgacctcg gcggcgaggg
      241 ccccgtcgaa tggatcgtgg accccattga cggcaccgtg aactatttct acggcatccc
      301 gcatttctgc gtctccatcg ccgcccgcat gcgcgagtcg ggtgaatacc tcctcggcgt
      361 cattcacgac cccatgcagg gcgagacctg gacggtggag aaaggcggcc cgcccaggct
      421 cgatggccag gcggtgcgcg tcagcccgcg tgaaaacatg agcgaggcca tcatcaccgc
      481 cggcttttca aagagcaaat ccgccctcga cgcgggcttc gagcgctacc gccgcatcag
      541 ctacgaggtc ttcaagacgc gcatgctcgg cagcgccgcg ctggcgctgg cctatgtcgc
      601 caccggccgg ctcgacgcct atgtcgagga gcagatcagc ttgtgggaca tcgccgctgg
      661 cgtgcaactc gtcgaggcgg cgggcggcag ggtcatcctg cgtcccagca ccgtgaagga
      721 ggacaccttc ttcatctgcg cctggaatgg ccgcctgccc atcgagcagt acctgtgagg
      781 cgggtcacag ctcccatccg gcgcggtatt tgcgcctgac cagcgcgttg gccgcctcgt
      841 tgttggggat cttcagcccc ggctcgtccc acaccaggtc ctggccgggg aaggcgctgg
      901 ccaggcagcc gaggagcacc gcctcggtca tcggccctgc gtagtcaaaa ttggcgctgg
      961 gctgcttgtc tcccttcagg cagcagtcaa tgtagtcacg ccaatggtcg cgcggctcca
     1021 gcttcgggta ttggaagccg gtgaatttcc cgcgcggata cagcatcggc gtgctgccat
     1081 gcggggcgag caaaacgccg tctgtgccga tgtagaggct gccctggccg gggaatttct
     1141 ccggatcagc caaagccatg acctccttcg gcgggcgcgc gtcgccatca taccacgtca
     1201 ccttcacggt gtcggcggcg gtgaactcgg tgcctttgaa ggtgtactcg accacggcat
     1261 tgacggccca gttggtgagg ttcaacgggg cggggccggt ggacttcacg ccgacaggcg
     1321 cggccaggct gagcgcgcgg aaccagccgc tgaacatatg gcagcccatg tctcccagag
     1381 tgccggtccc aaagtctcgc cgcttacgcc actggccggg gtggtagtat ttgtccatgt
     1441 agggccggtc ggctgcgggg ccgagccaga gctgccagtc gaagccctcc ggcaccgcgt
     1501 ccgcgcgctg cggcacggga tccatgtccc cccagaactt gttcgagaaa gtgtgcacct
     1561 ctttcacctt tccaatggtg ccctgctgaa tgatcgccac cgcgtgccgc tcggtgaagg
     1621 cggaggaaat ctggatgccc atctgcgtca tcaccttctt ctcgcgcgcc ttgagcatga
     1681 gttggcggca ctcgaacagg ttctgcgcca gcggcttctg gccgtacatg tgcttgccca
     1741 gctccagcac cctcatgccc atcggcccgt gcatgtggtc cggcgtggag acgttcaccg
     1801 aatcaatgtt tcccgcctcc ttctccagca gctcccgcca gtcctggtag caacgcgcgt
     1861 gggggaattt ctccttgatg cgggcgaagt tccgcgtgtc cacatccgcc acggccgtca
     1921 gctcccacat ctcatggttc gccatctggc tgatgtccgc ccacgacatg ccggacgcgc
     1981 cgaaggccgc gtgccgcagc ttgccattcg gggaggcggc cctcatgccg gtggtgacga
     2041 agggggcggc gaaaagtccg cccgctgcgc gggtgaggaa ctggcggcgt gtggtggtga
     2101 tggtctggct catgacgggc ggaaggtacg cgcaaaagcg ccgccgtctt tggaagttca
     2161 aaattcgaat ttcgaatttc gaatttcgca ctaccaccct tccatgtccc tcatcctcgg
     2221 cattgacctc ggcaccaccc actccctcgt cggcgtcatg gacagcggct tccccatcct
     2281 cctcgcggat gcggagggcg gccgcctcac gccctccgcc gtgcatttcg gcgcggacgg
     2341 cagcgtcacc gtcggcgcgc ccgccctgcg ccgtcggg
//
LOCUS       DAOMYJ010000436         2377 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Prosthecobacter sp. isolate AMR_MDS_5629
            NODE_17091_length_2377_cov_18.026270, whole genome shotgun
            sequence.
ACCESSION   DAOMYJ010000436 DAOMYJ010000000
VERSION     DAOMYJ010000436.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867850
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prosthecobacter sp. (wastewater metagenome)
  ORGANISM  Prosthecobacter sp.
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Verrucomicrobiaceae; Prosthecobacter.
REFERENCE   1  (bases 1 to 2377)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2377)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.30
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:34:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,742
            CDSs (total)                      :: 4,684
            Genes (coding)                    :: 4,676
            CDSs (with protein)               :: 4,676
            Genes (RNA)                       :: 58
            tRNAs                             :: 55
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2377
                     /organism="Prosthecobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17091_length_2377_cov_18.026270"
                     /isolate="AMR_MDS_5629"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1965333"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            77..334
                     /locus_tag="PLP58_22850"
     CDS             77..334
                     /locus_tag="PLP58_22850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ11435.1"
                     /translation="MNPSICRTLSTVCFQAGILSILLSIWIWWFLSGEGPEQQAHAER
                     FGIFIGLWAPTLLILSNRFDRYADKARPVPGLVDEKDGEQG"
     gene            complement(361..1518)
                     /locus_tag="PLP58_22855"
     CDS             complement(361..1518)
                     /locus_tag="PLP58_22855"
                     /inference="COORDINATES: protein motif:HMM:NF013755.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HRDC domain-containing protein"
                     /protein_id="HRJ11436.1"
                     /translation="MSDAPPAAPPEAAPGDYVFIHQPGELARWIAETERHMEALGEER
                     CCLDTEADSLHHYHEKLCLLQVACAGRFALVDPLAIPDVSGLLALLDRHELWFHGSDY
                     DLTMLRRTYGWEPRVVRDTQIAARLAGFRQFGLAALIHSVFGVQLSKASQKADWSRRP
                     LPVHMMDYAVDDVRYLIPLADHLMRILREKNRVAWFEESCRDLQVDVAARGAEAREDP
                     WRVQGSGRLHPKGLAILKAMWEWREGIARERDIPCYRIMSNKQMVAYAETFEAGGVMN
                     PPAGWRPRWKKEFHDLVASVFREGQGAWPQRVKKPKARLTDPQREHIDRLCAHRDKIA
                     AGLGIEGSLLGSRGTLESLVITGRDAVLMEWQRQLLADGLKMPGPPPGTLL"
     gene            complement(1515..>2377)
                     /gene="rho"
                     /locus_tag="PLP58_22860"
     CDS             complement(1515..>2377)
                     /gene="rho"
                     /locus_tag="PLP58_22860"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009959355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008186 - ATP-dependent activity, acting
                     on RNA [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HRJ11437.1"
                     /translation="HFDKLTAMFPNQRIILETGKTSPPSARILDLVAPLGKGQRGLIN
                     ASPRSGKTMLLKDIAKSIVKNHKEVTLIILLLDERPEEVTDFEESVSGCEIYSSTFDE
                     SPKRHAQLAEIVRERACRLVEQKKDVVILLDSLTRLARGYNANAGGKGRTMSGGMDSK
                     ALARPKKFFGAARNVEEGGSLTIIASALVETESRMDELIFEEFKGTGNMEATLDREIS
                     ERRIFPALHVLKSGTRRDDLLYHPEEFRRVSAIRKQLAQLPAVDALELLLRNIARTSN
                     NAEILLTGLK"
BASE COUNT          333 a          678 c          926 g          440 t
ORIGIN      
        1 gcgctttttg gctttcacgg ggcggggctt ggcgggggct tgcggcgggg ccgggtggtc
       61 cgtttaatgg gctgccatga atccctccat ctgccgcacg ctgagcacgg tctgtttcca
      121 ggccggcatt ctttcgatcc tgctttcgat ctggatctgg tggttcctga gcggggaggg
      181 gccggagcag caggcgcacg cggagcgctt cggcattttc atcggcctgt gggcgccgac
      241 tctgctgatc ctgtcgaacc gcttcgaccg ctacgcggac aaggcgcgcc cggtgccggg
      301 cctggtggat gagaaggacg gcgagcaggg ctgagcgcca ggcccgcgcg gccgcgcggg
      361 tcagaggagg gtgccgggcg gcgggccggg cattttcaac ccgtccgcga gcaactgccg
      421 ctgccattcc atgaggacgg cgtcccggcc ggtgatgacg aggctctcga gggtgccgcg
      481 ggagccgagc aggctgccct cgatgccgag cccggcggcg attttgtccc ggtgggcgca
      541 gaggcggtct atgtgctcgc gctgggggtc ggtgaggcgg gccttgggct ttttcacgcg
      601 ctggggccag gcgccctggc cttcgcggaa gacggaggcg acgaggtcgt ggaactcttt
      661 tttccagcgc gggcgccagc ctgcgggcgg attcatgacg ccgccggcct cgaaggtctc
      721 ggcgtaggcg accatttgct tgttcgacat gatgcggtag caggggatgt cgcgctcgcg
      781 ggcgatgccc tcgcgccact cccacatggc tttgaggatg gcgaggcctt tggggtggag
      841 cctgccgctg ccctggacgc gccagggatc ctccctggcc tccgcgccgc gcgcggccac
      901 gtccacctgc aagtcgcggc agctttcctc gaaccatgcg acgcggtttt tctccctgag
      961 aatgcgcatg aggtggtcgg cgagggggat gaggtagcgc acgtcgtcca cggcgtagtc
     1021 catcatgtgg acggggaggg ggcggcggct ccagtcggct ttttgggagg ctttggagag
     1081 ctggacgccg aagacggagt ggatgagggc ggcgaggccg aactggcgga aaccggcgag
     1141 gcgcgcggcg atctgggtgt cgcgcacgac gcgcggctcc cagccgtagg tgcggcggag
     1201 catggtcaga tcatagtcgg agccgtgaaa ccagagctcg tggcggtcca gcagcgcgag
     1261 cagcccgctc acatcgggga tggcgagcgg gtccacgagg gcgaagcggc ccgcgcacgc
     1321 cacctggagc aggcagagct tttcatgata gtggtggagg gagtcggcct cggtgtcgag
     1381 gcagcagcgc tcctcgccga gcgcttccat gtggcgctcg gtctcggcga tccagcgggc
     1441 aagctcgccg ggctggtgga tgaagacata atcccccggc gcggcttcgg ggggggctgc
     1501 tgggggagcg tcgctcattt cagtccagtg agcaggattt cggcgttgtt gctggtgcgg
     1561 gcgatgttgc gcaggaggag ctcgagggcg tccacggcgg ggagctgggc gagctgcttg
     1621 cggatggcgc tgacgcggcg gaactcctcg ggatgataga gcaggtcgtc gcggcgggtg
     1681 ccggatttga gcacatgcag ggcggggaag atgcgcctct cggagatctc gcgatccagg
     1741 gtggcctcca tgtttccggt gcctttgaac tcctcaaaga tgagctcgtc catgcggctc
     1801 tcggtctcga caagggcgga ggcgatgatg gtgaggctgc cgccctcctc gacgttgcgc
     1861 gcggcgccga agaatttctt cggcctggcc agcgccttgg agtccatgcc gccggacatg
     1921 gtgcggccct tgccgccggc gttggcgttg tagccgcgtg cgaggcgtgt gagggagtcg
     1981 agcaggatga cgacgtcctt tttctgctcg acgaggcggc aggcgcgctc gcggacgatc
     2041 tcggcgagct gggcgtggcg cttggggctt tcgtcaaagg tggagctgta gatctcgcag
     2101 ccgctgacgg actcctcgaa gtcggtgacc tcctcggggc gctcgtcgag caggaggatg
     2161 atgagggtga cctccttgtg gttcttgacg atggatttgg cgatgtcctt gagcagcatc
     2221 gtcttgccgg agcgcgggga ggcgttgatg aggccgcgct ggcctttgcc gaggggcgcc
     2281 acgagatcaa ggatgcgcgc ggatggaggg gaggtcttcc ctgtctcgag gatgatccgc
     2341 tggttgggga acatggccgt gagcttgtcg aaatgca
//
LOCUS       DAOMYJ010000437         2373 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Prosthecobacter sp. isolate AMR_MDS_5629
            NODE_17145_length_2373_cov_19.753236, whole genome shotgun
            sequence.
ACCESSION   DAOMYJ010000437 DAOMYJ010000000
VERSION     DAOMYJ010000437.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867850
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prosthecobacter sp. (wastewater metagenome)
  ORGANISM  Prosthecobacter sp.
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Verrucomicrobiaceae; Prosthecobacter.
REFERENCE   1  (bases 1 to 2373)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2373)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.30
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:34:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,742
            CDSs (total)                      :: 4,684
            Genes (coding)                    :: 4,676
            CDSs (with protein)               :: 4,676
            Genes (RNA)                       :: 58
            tRNAs                             :: 55
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2373
                     /organism="Prosthecobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17145_length_2373_cov_19.753236"
                     /isolate="AMR_MDS_5629"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1965333"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            123..1010
                     /locus_tag="PLP58_22865"
     CDS             123..1010
                     /locus_tag="PLP58_22865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009958032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD/SigA"
                     /protein_id="HRJ11438.1"
                     /translation="MELDGGIKIYLREIGKTDLLTPEQEVDLAERIKRGDPEARSHMI
                     RANLRLVVKIAQDYANYGLPLLDLISEGNIGLMKAVERFDPNKGGKLSTYAAWWIKQS
                     IKRALANQSKTIRLPVHMVDKISKMRRVAMAMSEELGREPTDDELSEEIGIDRAKLSQ
                     LKVASMRPASLDAPISDDDSTEFGEIVGDENAQTPFDLLSHKNMHSQLDGLLTVLDER
                     ERKIIDARFGLAGQKPRTLEEVGQEFGVTRERIRQLQNIALRKLRRALQKKEDPIPKA
                     LRNAGKKKKKAAAAAEDKK"
     gene            complement(1019..1627)
                     /locus_tag="PLP58_22870"
     CDS             complement(1019..1627)
                     /locus_tag="PLP58_22870"
                     /EC_number="2.7.7.77"
                     /inference="COORDINATES: protein motif:HMM:NF024214.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0061603 - molybdenum cofactor
                     guanylyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor guanylyltransferase"
                     /protein_id="HRJ11439.1"
                     /translation="MPPFAALLLAGGRSRRMGRDKAFLDWQGVPLWRWQFEKLAALRP
                     ARLLLSCRAEQRSEMTQPPSDIEWFVDPPGYDSGPLGILTRALELAGMPVLALAVDMP
                     LMPAGEIVRCWALEAGGMGFCFESKHGHEPLAAVYTPALLPLMRKSLVAGSMGLQPLI
                     LEAVLRGLMRTRKGVKTESSWFTNLNTLDDVEHEKGGHFCPP"
     gene            complement(1636..>2373)
                     /locus_tag="PLP58_22875"
     CDS             complement(1636..>2373)
                     /locus_tag="PLP58_22875"
                     /inference="COORDINATES: protein motif:HMM:NF012742.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HRJ11440.1"
                     /translation="LREKLAAAEHALELLTLPAAFGPDGEKSLKHLEKRGQEAVWLLH
                     LAPERVQRWFAQRGFPCLVAGSAAPGVELPSVDLDHRATCRHAGGLLAAKGKTRPVLL
                     LPDTHAPGDKESEQGFLEGYTRQAGKPLILRHDGTVEGLCAQVSAAARGRAPADAFVV
                     GRSAHALTVLTCLLGEGCRVPGEVAVISRDHDLFLAHTTPEMARYASDPSKWARVIFK
                     CLLQLMEGVPLRTQHRLVPDFIRGGTL"
BASE COUNT          512 a          818 c          665 g          378 t
ORIGIN      
        1 gagccacccg gatgaggcgc acgtcgttgc tcgtggccgg ctgctgcttg ctgcccctga
       61 atttcatcag gaaatcaaga atcccaaatc tgcatcgtac caacccttga aacctttcat
      121 ctatggaact cgacggaggc attaaaatct acctgcgtga gatcggcaag actgatttgc
      181 tgactccgga gcaggaggtt gatctcgccg agcgcatcaa gcgcggcgac ccagaggcgc
      241 gctcgcacat gattcgcgcc aacctccgcc tcgttgtcaa aatcgcccag gattacgcga
      301 actacggcct gcccctgctc gacctcatct ccgagggcaa catcggcctc atgaaggcgg
      361 tggagcgctt cgaccccaac aagggcggca aactctccac ttacgcggca tggtggatca
      421 aacagtccat caagcgcgcc ctggccaacc agtccaagac catccggctg cccgtccaca
      481 tggtggacaa gatcagcaag atgcgccgcg tggccatggc catgtctgag gaactcgggc
      541 gcgagccgac ggatgacgaa ctatcagaag aaatcggcat tgaccgcgcc aagctcagcc
      601 agctcaaagt ggcctccatg cgccccgcct cgctcgacgc gcccatcagc gatgatgaca
      661 gcaccgagtt tggagagatc gtcggcgatg aaaacgcgca gaccccattc gacctcctca
      721 gccacaagaa catgcacagc cagctcgacg ggctgctgac cgtgctggat gagcgtgaac
      781 gcaagatcat tgacgcgcgc ttcggcctcg ccggacaaaa gccgcgcacg cttgaggaag
      841 tgggtcagga attcggagtc acacgcgagc gtattcgtca gcttcaaaac atcgctctgc
      901 gcaagctgcg ccgcgcgctt cagaagaagg aagatccaat tccaaaagcg cttcgcaacg
      961 ccggcaagaa aaaaaagaag gctgccgccg ctgcggaaga caaaaagtga ttcatccatc
     1021 aaggcgggca gaaatgcccg cctttttcat gctcgacatc atccagcgtg tttaaatttg
     1081 taaaccagga agactctgtt ttcactccct tcctggtcct catcaaccct cgcagcactg
     1141 cttcaaggat caggggttgc aaccccatac tgccagccac caggctcttt cgcatgagcg
     1201 gcaacaaagc aggcgtataa actgcggcca gcggctcatg gccatgcttt gactcaaagc
     1261 aaaaacccat gccgcccgct tccaacgccc agcacctcac gatctcgccc gcgggcatga
     1321 gcggcatgtc cacagccagc gcgagcaccg gcatgcccgc caactctagc gcgcgagtca
     1381 aaatacccaa cggccccgaa tcataaccgg ggggatccac aaaccactcg atgtccgatg
     1441 gtggttgagt catttcagat cgctgttccg cgcggcagga gagcagcagg cgcgcgggac
     1501 gcagtgcggc caacttctca aactgccatc tccacaaagg cacgccctgc caatccagaa
     1561 aagccttgtc cctgcccatt cggcgtgagc ggccaccggc aagcagcagc gcggcgaaag
     1621 gcggcatctc cgcgctcaca gcgtgcctcc ccggatgaag tcaggcacca gacggtgctg
     1681 cgtcctgagc ggcacgccct ccatgagttg cagcaggcat ttgaaaatga cacgcgccca
     1741 cttcgagggg tcgctggcgt agcgcgccat ctccggagtg gtgtgagcta ggaaaagatc
     1801 atggtcccgc gagatcaccg ccacctctcc aggcacgcgg cagccctcgc caagcaggca
     1861 ggtgagcacc gtcagcgcat gagccgagcg gcccaccaca aaggcatccg ccggcgcccg
     1921 gccccgcgcc gccgcgctca cctgggcgca gagtccctcc accgtgccat cgtgccgcag
     1981 gatgagtggc ttgccggcct ggcgcgtgta gccttccaga aagccctgct cgctctcctt
     2041 gtcccccggc gcgtgcgtgt ccggcagcag cagcaccgga cgcgttttcc ccttcgccgc
     2101 cagcaggccg cccgcatggc ggcaagtggc gcggtggtcc aggtccaccg agggcagctc
     2161 cactccggga gccgctgatc ccgccaccag gcaggggaag ccccgctgcg cgaaccagcg
     2221 ctgcacacgc tcaggcgcca gatgcaaaag ccacaccgcc tcctggccgc gcttctccag
     2281 atgtttgagg gacttctccc cgtccgggcc gaacgccgcc ggcagcgtca gcagctccag
     2341 cgcgtgctcc gccgccgcca gcttctcccg cag
//
LOCUS       DAOMYJ010000438         2367 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Prosthecobacter sp. isolate AMR_MDS_5629
            NODE_17192_length_2367_cov_19.734429, whole genome shotgun
            sequence.
ACCESSION   DAOMYJ010000438 DAOMYJ010000000
VERSION     DAOMYJ010000438.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867850
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prosthecobacter sp. (wastewater metagenome)
  ORGANISM  Prosthecobacter sp.
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Verrucomicrobiaceae; Prosthecobacter.
REFERENCE   1  (bases 1 to 2367)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2367)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.30
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:34:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,742
            CDSs (total)                      :: 4,684
            Genes (coding)                    :: 4,676
            CDSs (with protein)               :: 4,676
            Genes (RNA)                       :: 58
            tRNAs                             :: 55
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2367
                     /organism="Prosthecobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17192_length_2367_cov_19.734429"
                     /isolate="AMR_MDS_5629"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1965333"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1377
                     /locus_tag="PLP58_22880"
     CDS             <1..1377
                     /locus_tag="PLP58_22880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009958870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1501 domain-containing protein"
                     /protein_id="HRJ11441.1"
                     /translation="DQAARSLLAREGWAAAQANGLPDLPHHFAKAKRVIYLFQNGAPS
                     HVDLFDHKPKLREWHGRQIPDSVAAGKRFSTMTGNQTDRPVLGEITHFSRHGQSGATV
                     SDFLPRTAEIADELCFIKSMHTEAVNHAPGITFFLTGSEMAGRPSMGAWLTYGLGRDS
                     EELPSYVVMTSRDKEASCGQIFYDFYWGSGFLPTKYQGVKFRGSGDPVLYLSNPEGMS
                     AQVRRGVLDDLARLNEMKLAEHGDPEIATRIAQYEMAYRMQTSVPELTDLSKEPKHIL
                     EMYGPDVLKQGSYAYNCLMARRLAERGVKFIQLMHAGWDQHRNLNSQLKIQCQDTDAP
                     SAALVKDLKQRGLLDDTLVIWGGEFGRTPFLQGKIEETKQWGRDHHPYAFTLWMAGGG
                     VRAGHSHGASDEFGFSAVEDKVHVHDFQATVLHLLGIDHERLTYKFQGRYFRLTDVHG
                     HVVRQVLA"
     gene            1423..>2367
                     /locus_tag="PLP58_22885"
     CDS             1423..>2367
                     /locus_tag="PLP58_22885"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HRJ11442.1"
                     /translation="MPRLPATLCLIVCLHMAARGQTFEDTLGLRTEWITLDEAVGRVL
                     ENSLDVRIERLNWAVSERQTEAAWAKFDPAWFMNSTWRDASLPQNTLDFVQTGGGFVV
                     LDEPNIFRQQSLLSQAGFEGLLPTGTQYRLFAGAGEFRNDLNRQRPPAIFYPEYAAAV
                     GGTITQPLLRGFGLGVNLAEVRVGRKNQEIALRQWEARLQRSVAQVVADYFDLIFAVE
                     DLEVKRGVTAFARRLVLENEKRLEAGQLSPADVQEAEVAVAVSREEVIAALGFAVEKQ
                     RSLKTQILGSMREGEGVVFLPRDSLPVIAPRTERAPLLA"
BASE COUNT          405 a          686 c          872 g          404 t
ORIGIN      
        1 gatcaggcag cccgttcgct gctggcgcgg gagggctggg cggcggcgca ggcgaacggg
       61 ctgcctgatc tgccgcatca tttcgcgaag gcgaagcggg tgatttatct tttccaaaac
      121 ggcgcgccct cgcatgtgga cctctttgat cacaagccga agctgcggga gtggcatggg
      181 cggcaaatcc cggattcggt ggcggcgggg aagcgcttca gcacgatgac gggcaatcaa
      241 accgaccggc ctgtgctggg ggagatcacg catttttccc gccacggtca gagcggggcg
      301 acggtgtcgg acttcctgcc gcgcacggcg gagatcgcgg acgagctgtg cttcatcaag
      361 tccatgcaca cggaggcggt gaatcatgcg ccgggcatca ccttctttct caccggctcg
      421 gaaatggcgg gccggccgag catgggggcg tggctgacgt acgggctggg gcgggacagc
      481 gaggagctgc cctcctatgt ggtgatgacc tcccgcgaca aggaggcgag ctgcggccag
      541 atcttttatg acttctactg gggcagcggt ttcctgccga cgaagtatca gggggtgaag
      601 tttcgcggca gtggcgaccc ggttttatac ctctccaatc cagaaggcat gagcgcgcag
      661 gtgcggcgcg gggtgctgga tgatctggcg cggctgaatg agatgaagct ggcggagcac
      721 ggggacccgg agatcgccac gcgcatcgcg caatacgaga tggcctaccg gatgcagacg
      781 agcgtgccgg agctgacgga cctttccaag gagccgaagc acattctcga gatgtacggg
      841 ccggatgtgc tgaagcaggg cagctatgcg tacaattgcc tgatggcgcg gcggctggcg
      901 gagcgggggg tgaagttcat ccagctcatg cacgcggggt gggaccagca ccggaatctg
      961 aacagccagc tcaagatcca gtgccaggac acggacgcgc cgagcgcggc gctggtgaag
     1021 gatttaaaac agcgcggtct gctggatgac acgctggtga tttggggcgg ggagtttggc
     1081 cgcacgccgt ttttgcaggg gaagatcgag gagacgaagc aatggggccg cgaccatcat
     1141 ccgtatgcct tcacgctctg gatggcgggc ggcggggtga gggccggcca ctcccacggg
     1201 gcgagtgatg agttcggctt cagcgcggtg gaggacaagg tgcatgtgca tgacttccag
     1261 gcgacggtgc tgcatctgct gggcattgat cacgaaaggc tgacctacaa gttccagggg
     1321 cggtatttcc ggctcacgga tgtacacggg catgtggtga ggcaggtgct ggcgtgagca
     1381 ccgccgcctt gaaatatccc cgcgccgccg ccaacatggc gcgtgccgcg tcttccagcc
     1441 accctctgcc tcatcgtctg cctgcacatg gcggcgcgcg ggcagacctt tgaggacacg
     1501 ctggggctgc ggacggagtg gatcacgctg gacgaggcgg tggggcgcgt gctggaaaac
     1561 agcctcgatg tgcgcatcga gcgcctgaac tgggccgtga gcgagcggca gacggaggcc
     1621 gcctgggcga aattcgaccc cgcctggttc atgaactcca cctggcggga tgccagcctg
     1681 ccgcagaaca cgctggactt tgtgcagacc ggcggcggct ttgtggtgct ggacgagccg
     1741 aacatcttcc gccagcagag tctgctgagc caggccggtt tcgaggggct gctgcccacg
     1801 ggcactcaat accggctgtt cgcgggcgcg ggcgagtttc gcaatgatct gaaccgccag
     1861 cgcccgcccg ccattttcta tccggaatac gccgccgcgg tggggggcac gatcacccag
     1921 cctctgctgc gcgggtttgg cctgggggtg aacctggccg aggtgcgcgt gggcaggaag
     1981 aaccaggaga tcgccctgcg ccagtgggag gcgcgcttgc agcgctcggt ggcgcaggtg
     2041 gtggcggatt attttgacct catcttcgcc gtggaggacc tggaggtgaa gcgcggcgtc
     2101 accgcctttg cccggcggct ggtgctggaa aatgaaaagc gcctggaggc cggccagctc
     2161 tcccccgccg atgtgcagga ggcggaggtg gccgtggccg tgagccggga ggaggtcatc
     2221 gccgcgcttg gcttcgcggt ggagaagcag cgctccctca agacgcagat cctcggctcc
     2281 atgcgcgagg gcgagggcgt ggtcttcctg ccgcgggact cgctgccggt catcgcgccg
     2341 cgcacggagc gcgccccgct gctggcc
//
LOCUS       DAOMYJ010000439         2366 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Prosthecobacter sp. isolate AMR_MDS_5629
            NODE_17202_length_2366_cov_25.491129, whole genome shotgun
            sequence.
ACCESSION   DAOMYJ010000439 DAOMYJ010000000
VERSION     DAOMYJ010000439.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867850
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prosthecobacter sp. (wastewater metagenome)
  ORGANISM  Prosthecobacter sp.
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Verrucomicrobiaceae; Prosthecobacter.
REFERENCE   1  (bases 1 to 2366)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2366)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.30
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:34:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,742
            CDSs (total)                      :: 4,684
            Genes (coding)                    :: 4,676
            CDSs (with protein)               :: 4,676
            Genes (RNA)                       :: 58
            tRNAs                             :: 55
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2366
                     /organism="Prosthecobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17202_length_2366_cov_25.491129"
                     /isolate="AMR_MDS_5629"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1965333"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            234..>2366
                     /locus_tag="PLP58_22890"
     CDS             234..>2366
                     /locus_tag="PLP58_22890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ11443.1"
                     /translation="MFQLPYAGLAEDKVSAIPASQIGAEAQKQYRGEKISIQQEPGGI
                     RLKALFQDLEGLATSEGLLMESRGREDGAGATRFRVVASEVGRDGGRMALPESGTVRM
                     GDGSVLFAREGLTEEYRVSMDGVRQDFVLRERPAGEGALKVTLRVTGAVAENARYGAK
                     LTLPSGREIAYSRLFASDAAGQELQAGISAHGGALVIEVDDRDAVYPVRIDPTFSDAD
                     WYSLGGVPGANDIVYATAVDGSGNLYIGGDFTVVGETQASRIAMWDGSTWSQVGAGLN
                     NIVYSLAYSGGVLYVGGEFTAAVGGASNSLKRLATWNGTAFAQVGGAGVNQGFVREIM
                     LDGTDIIIGGSFTTVASPAQTVNRIARWNGVAWSGFGSGFDNTVRALAKDSGGNIYAG
                     GSFSQEVGGAANGLMRVAMWNGAAWSALGAGFNNIVRDLAIDGADTLYAGGEFSSLVG
                     GAANSLTRVARWDGATWSTLGATAGLSNFVYTLAWDEAAETLYAGGAFTSTTGGVASS
                     MRRVGRWSRASNTWSAMGIGTTPGPGVEDGNVWTLTLAAGGVYLGGDFTEADDWGYSR
                     IAFWDSGTNEFSGLASGLNNVVNALAVNGTDVYLGGDFIRAGDIQASSIVKWDGSDFE
                     ALGAGLNGSVIVLLADGSDVYVGGTFTSTLGGPTNSLRNLARWDTVADAWNAVGLGVN
                     STVRSLAKDGMGNLYVGGAFTTVSLGPTI"
BASE COUNT          418 a          632 c          889 g          427 t
ORIGIN      
        1 ccgagccggg gttgctcggg gttgctgttt ttgaggcgct gactaagggt gggttagtaa
       61 ggggtgcgga ttttgccatg cagaactgtg acgcaattct gcgattcgta gcaaaaatcc
      121 gtcattcgcc gcttttagtt cgaaccttta atggcacatt gaagcgtatg agaatactcc
      181 cgaacttaag attggccgag tcgtcgtttg caaaagcatt cattctgagt ctgttgttcc
      241 aactgcccta tgcgggttta gctgaggata aagtttcggc catacccgcc agccagatcg
      301 gcgcggaggc gcagaagcag tatcgaggcg agaagatctc gatccagcag gagccgggcg
      361 gcatccgcct caaggcgctg ttccaggatt tggagggtct ggcgacgagc gagggcctgc
      421 tgatggagtc gaggggccgt gaggacggcg ctggcgcgac gaggttccgc gtggtggcgt
      481 ccgaagtcgg gcgtgacggc ggcaggatgg cgctgccgga gtctggcacg gtgcgcatgg
      541 gggacggcag tgtgctgttc gcccgcgagg gtctgacgga ggaataccgg gtgagcatgg
      601 atggcgtgcg gcaggacttt gttctgcggg aaaggccggc cggagagggt gccttgaagg
      661 tgacgctgcg cgtgacgggg gctgtggcgg agaatgcccg ctacggggcg aagctgacgc
      721 tgccgagcgg gcgcgagatc gcctacagca ggctgttcgc gagtgacgcg gctggccagg
      781 agctgcaggc gggcatttcc gctcatggcg gcgcgctggt catcgaggtg gatgaccggg
      841 acgcggtgta tcctgtgcgc attgacccga ccttcagtga cgcggactgg tatagcctcg
      901 gcggagtgcc cggagccaat gacatcgtgt atgcgacggc ggtggacggg agcgggaatc
      961 tttacattgg cggcgacttt accgtggtgg gcgagacaca ggccagccgg atcgcgatgt
     1021 gggacggcag cacctggagc caggtgggcg cggggttgaa caacatcgtg tattccctgg
     1081 cctactccgg gggcgtgctg tatgtgggcg gcgagttcac cgcggcggtc ggaggtgcga
     1141 gcaacagcct gaaacgcctc gccacctgga acggcacagc ctttgcccag gtgggcggag
     1201 cgggcgtgaa ccagggattt gtgcgcgaga tcatgctcga tggaacggac atcatcatcg
     1261 gcggctcctt caccacggtg gcgagcccgg cgcagacagt gaaccgcatc gcgcggtgga
     1321 acggggttgc ctggagcggc ttcggcagcg gctttgacaa cacagtgcgc gcgctggcga
     1381 aggacagcgg cggcaacatc tacgcaggcg gcagctttag ccaggaagtg ggcggcgccg
     1441 caaacggtct catgcgtgtg gcgatgtgga atggcgcggc ctggagcgcg ctgggagccg
     1501 gctttaacaa catcgtcagg gacctggcga ttgacggtgc tgacacgctg tatgccgggg
     1561 gtgagttctc ctccctggtg ggtggcgcgg ccaacagcct gacccgcgtg gcccgctggg
     1621 atggcgcgac ttggagcaca ctgggcgcca cggcgggtct gagcaacttc gtgtataccc
     1681 tggcctggga cgaggccgcc gagacactct acgcgggcgg ggcattcacc tcgaccaccg
     1741 gtggcgtggc cagcagcatg cggcgagtgg ggcgctggag cagggcgtca aacacctggt
     1801 cggcgatggg gatcggcaca acgcccggac ccggcgtgga ggacggcaat gtctggacgc
     1861 tgacgctggc ggccggcggg gtgtatctgg gtggcgactt cacggaggcg gatgactggg
     1921 gctactcccg catcgccttc tgggacagcg gcaccaacga gttcagcgga ctggcatccg
     1981 gcctgaacaa tgtggtcaac gcgctcgccg tgaatggaac ggatgtttac ctgggcggcg
     2041 atttcatccg cgccggtgac atccaggcaa gcagcatcgt caaatgggat ggcagtgact
     2101 tcgaggcgct gggggcggga ctcaacggtt cggtgatcgt cttgctggcg gacggcagcg
     2161 acgtgtatgt gggtggaact ttcacctcaa ccctgggagg cccgaccaac agcctgcgca
     2221 atctcgcccg ctgggacacg gtggcggacg cctggaatgc ggtggggctg ggtgttaact
     2281 ccacagttcg ctcgctggcc aaggatggca tggggaattt gtatgtcgga ggggcattca
     2341 ccacggtcag cttaggcccg acgata
//
LOCUS       DAOMYJ010000440         2351 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Prosthecobacter sp. isolate AMR_MDS_5629
            NODE_17380_length_2351_cov_14.539199, whole genome shotgun
            sequence.
ACCESSION   DAOMYJ010000440 DAOMYJ010000000
VERSION     DAOMYJ010000440.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867850
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prosthecobacter sp. (wastewater metagenome)
  ORGANISM  Prosthecobacter sp.
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Verrucomicrobiaceae; Prosthecobacter.
REFERENCE   1  (bases 1 to 2351)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2351)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.30
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:34:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,742
            CDSs (total)                      :: 4,684
            Genes (coding)                    :: 4,676
            CDSs (with protein)               :: 4,676
            Genes (RNA)                       :: 58
            tRNAs                             :: 55
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2351
                     /organism="Prosthecobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17380_length_2351_cov_14.539199"
                     /isolate="AMR_MDS_5629"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1965333"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..657)
                     /locus_tag="PLP58_22895"
     CDS             complement(<1..657)
                     /locus_tag="PLP58_22895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009965205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HRJ11444.1"
                     /translation="MTELISKGGPLVWLLMACMGMSLAIFLERLAYYHRAGMNIGEFL
                     SGLAALIRRRNYAEALQEALATRVPAGRVIHAALLRHHAPREQLKEIVQEAGQLEVPR
                     LERYLGILNAIAHVAPLIGLLGTVIGLLNSFTNLSSAQGVSTPAEVAAGLYQSLVTAA
                     LGLVVAIPAYLCYAFLSARARSIMHDLERAGVEIVNLIEDARGGDNIVEFRQAGAEEA
                     A"
     gene            792..1223
                     /locus_tag="PLP58_22900"
     CDS             792..1223
                     /locus_tag="PLP58_22900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ11445.1"
                     /translation="MPTETLRVLLAEPSLAYRRVIREALESFRRCQVDDTPTGERAFE
                     MALQRPYNLLILALPLSDISGLLLDRLIALAFPRVHSGSLTAPPVIFIARQEDAPAFQ
                     ASQRDARHRGLLHYPPKLDALLALTSGLLPEHSPALPPHVP"
     gene            1213..1911
                     /locus_tag="PLP58_22905"
     CDS             1213..1911
                     /locus_tag="PLP58_22905"
                     /inference="COORDINATES: protein motif:HMM:NF012900.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylanthranilate isomerase"
                     /protein_id="HRJ11446.1"
                     /translation="MFPEPARLGVKICGLTSESQARAVIALGADALGINFWPRSKRHL
                     PPEQAAPWLPALHPRTTLVAVLVNAGPELVAQILGENLARILQLHGDETPSQVAALME
                     RGIPVIKALQVRDRACLDAIGRYPCQTLLLDAFNPGHYGGGGATFPWELAVIARDMFP
                     EKRLILSGGLTPQNVRQAVVETRPAAVDVASGVESSPGVKDLARVAEFIAQARAGAAS
                     PGSPASPPLPSSLR"
BASE COUNT          354 a          921 c          700 g          376 t
ORIGIN      
        1 ggccgcctcc tcggcgccgg cctggcggaa ttcgacgatg ttgtccccgc ccctggcatc
       61 ctcgatcagg ttcacgatct ccaccccggc gcgctccagg tcgtgcatga tggagcgggc
      121 gcgggcgctg aggaaggcgt agcaaaggta ggccgggatg gccaccacga ggccgagcgc
      181 ggcggtgacg aggctttgat aaaggcccgc cgccacctcc gccggagtac tcacgccctg
      241 ggccgaggag aggttggtga agctgttgag caggccgatc actgtgccca gcaggccgat
      301 gagcggcgcc acatgggcga tggcgttgag gatgcccagg tagcgctcca ggcgcggcac
      361 ttcgagctgg ccggcctcct ggacgatctc cttgagctgc tccctgggcg cgtgatggcg
      421 cagcagcgcg gcatggatga cgcgcccggc gggcacgcgc gtggccagcg cctcctgcag
      481 cgcctcggcg taattgcgcc ggcggatcag cgcggccagg ccggaaagga actcgccgat
      541 gttcatgccc gcgcggtggt agtaggccag gcgctccagg aaaatggcca gggacatgcc
      601 catgcaggcc atcaacagcc agaccagcgg tcctcctttg gaaatgagtt ctgtcatgtc
      661 agtgcggtgc tcgcgaacct gccgcgcggc cgggaacacc cggcgtcacg ctgagcaagt
      721 tttatgcctt gggattgaat ttgcaaatat ggcgctgaaa atagcctccc tcctccgcga
      781 tggaaagaca tttgccaacc gaaactctgc gcgtcctgct ggccgagccc agcctggcct
      841 accggcgcgt catccgcgag gcgctggagt ccttccgccg ctgccaggtg gacgacacgc
      901 cgacggggga gcgcgccttc gagatggcgc tccagcgccc ctacaacctg ctcatcctgg
      961 ccctgcccct gtctgacatc tccggcctgc ttctggaccg cctcatcgcc ctggcctttc
     1021 cccgcgtcca cagcggcagc ctcaccgcgc cgcccgtcat cttcatcgcc cgccaggagg
     1081 acgcacccgc cttccaagcc tcccagcgcg acgcccgcca ccgcggcctg ctccactatc
     1141 cgccgaagct cgacgccctg ctcgcgctca ccagcggcct gctgccggaa cactcccccg
     1201 cccttccccc tcatgttccc tgaacccgcc cgcctcggcg tcaaaatctg cggcctcacc
     1261 agcgaaagcc aggcgcgcgc cgtcatcgcc ctcggcgccg acgccctcgg catcaacttc
     1321 tggccccgct ccaagcgcca cctccccccg gagcaggccg ccccctggct gcccgccctg
     1381 cacccgcgga ccaccctcgt cgccgtcctg gtcaacgccg gcccggagct cgtcgcgcaa
     1441 atcctcggcg aaaacctcgc ccgcatcctc cagctccacg gggatgagac cccttcccag
     1501 gtcgccgccc tcatggagcg cggcatcccc gtcatcaaag ccctccaggt gcgcgaccgc
     1561 gcctgcctcg acgccatagg ccgctacccc tgccagaccc tcctgctcga tgccttcaac
     1621 cccggccact acggcggcgg cggcgccacc ttcccctggg agctcgccgt catcgcccgc
     1681 gacatgtttc ctgaaaagcg gctcatcctc tccggcgggc tgacgccgca aaacgtgcgc
     1741 caggcagtcg tcgaaacccg ccccgccgcc gtggacgtgg ccagcggcgt cgaatccagc
     1801 cccggcgtca aagacctggc ccgcgtggcg gaattcatcg cccaggcgcg cgccggagcg
     1861 gcctcccccg gcagcccggc cagccccccg ctcccatcat ccctgcgctg acaggcaggc
     1921 tcccggtccc ctcccacccc gccaaggtgc gccgtcacct tcacaaactc cccgcgcacc
     1981 tcgccagggt gcgccgtcac cttcacaaac ttcccgcgta cttcgccagg gtgcgccgtc
     2041 actttcacaa acttcccgcc tgcctcactg tggttatgac tcttggcacc ataaaaaggc
     2101 ggggtttttg gggtcagcct ttgtcggcac atggatccca tccagccgcc cttcctccat
     2161 tttcccccgc ttcccgtctt gccgcgcagg gagctgcctg ctaaacctgc gctcctgcaa
     2221 tgacacgcgc ccaatggttg accgccggct cctggctcct gctgttctgg ctgctggccg
     2281 gaatggtctg ggcgccggcg ctggagcggc gtctggagcg gcgggcggca gagctgctgg
     2341 cggaggcgga c
//
LOCUS       DAOMYJ010000441         2331 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Prosthecobacter sp. isolate AMR_MDS_5629
            NODE_17595_length_2331_cov_15.760545, whole genome shotgun
            sequence.
ACCESSION   DAOMYJ010000441 DAOMYJ010000000
VERSION     DAOMYJ010000441.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867850
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prosthecobacter sp. (wastewater metagenome)
  ORGANISM  Prosthecobacter sp.
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Verrucomicrobiaceae; Prosthecobacter.
REFERENCE   1  (bases 1 to 2331)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2331)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.30
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:34:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,742
            CDSs (total)                      :: 4,684
            Genes (coding)                    :: 4,676
            CDSs (with protein)               :: 4,676
            Genes (RNA)                       :: 58
            tRNAs                             :: 55
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2331
                     /organism="Prosthecobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17595_length_2331_cov_15.760545"
                     /isolate="AMR_MDS_5629"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1965333"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..604
                     /locus_tag="PLP58_22910"
     CDS             <1..604
                     /locus_tag="PLP58_22910"
                     /inference="COORDINATES: protein motif:HMM:TIGR02603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HRJ11447.1"
                     /translation="LKAADPLARGSAIVAAGGDEIHPDELASWFSAQSRAKAVAEAPK
                     IQHFNADRQKVVTRYLKEAAPLQGDAARGMLVYQKACMACHQMRGQGAELGPDLATVA
                     GKPVEQILEAVFDPNRAVELRNAATQITKKDGGLVLGLIALETPHAVTLRLPGGVELP
                     VPRAEIQEMKTLQTSLMPDGLEAVLSAQDTADLLAAIQGK"
     gene            complement(621..1991)
                     /locus_tag="PLP58_22915"
     CDS             complement(621..1991)
                     /locus_tag="PLP58_22915"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009959344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HRJ11448.1"
                     /translation="MQLTQLLDSGLLAFCLWFAHYFRSRTLPVFIPDLVEIPPIEQLY
                     WLMALVVPFTPLILEERGFYSGLVEKTTAQSLRQLMEALAGISLAVGALVVFLKWEVP
                     SRSVAMLAIVMSGSALLLREEVQRARLRRRLASGKGRERVLLAGAGDDMRQLMEKVPA
                     EQRAHIEVAGCVDITRQPISDLVAAMHEHSVARVLFAAQHVHFGKIEEAVQACETEGV
                     EAWIAADFFQTAISRPTFDVLGGKLMLVFHSTPQVSWALLFKDLADRVVAALLLVLTL
                     PLWLAAVIGIRLASPGGPVFFRQERAGHYGRPFMMWKFRTMHPDAEARRAELEFQNEM
                     RGPAFKIANDPRVFAFGRWLRKFSIDELPQLLNVLRGEMSLVGPRPLPVYEIGKIEKH
                     AQRRRLSVKPGLTCLWQITGRNGIRNFEDWVALDLQYIDNWSLWLDAKILLKTPAAVL
                     RGVGAS"
     gene            2073..>2331
                     /locus_tag="PLP58_22920"
     CDS             2073..>2331
                     /locus_tag="PLP58_22920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ11449.1"
                     /translation="MPQLLSSSNLLLSSRPLTVAVIPDAATLALWQGMEPEARAAACD
                     VIELRLDTLQTAPGDIRASLQGNATPVLLTARHPAEGGQGSA"
BASE COUNT          401 a          741 c          835 g          354 t
ORIGIN      
        1 gctgaaggcg gccgatccgc tggcgcgcgg ctcggccatc gtggccgcgg gcggggatga
       61 gatccacccg gacgagctgg cctcctggtt ctccgcgcag agccgggcaa aggcggtggc
      121 ggaggcgccg aagatccagc atttcaatgc cgaccgccag aaggtggtga cgcggtatct
      181 gaaggaggcc gcgcctttgc agggggatgc cgcgcgcggg atgctggtct atcaaaaggc
      241 ctgcatggcc tgccatcaaa tgcgcggcca gggcgcggag ctggggccgg acctggcgac
      301 ggtggcgggc aagccggtgg agcagatttt ggaggccgtc tttgacccca accgcgccgt
      361 ggagctgcgc aacgcggcca cgcagatcac gaagaaggac ggcgggctgg tgctgggcct
      421 catcgcgctg gagacgccgc acgcggtgac gctgcgcctg cccggcgggg tggagctgcc
      481 cgtgccgcgg gcggagattc aggagatgaa gaccctgcaa acctccctca tgccggacgg
      541 cctggaggcc gtgctcagcg cccaggacac ggcggacctg ctggcggcga tccaggggaa
      601 ataagcgcgc gccagccggc tcacgaggcg cccacgccgc gcaggacggc ggcgggggtt
      661 ttgagcagga ttttggcgtc gagccagagg gaccagttgt ctatgtattg caggtccagg
      721 gccacccagt cctcgaagtt gcggatgccg ttgcggccgg tgatctgcca caggcaggtg
      781 aggccgggct tcacgctgag ccggcggcgc tgggcgtgct tttcgatttt gccgatctca
      841 tacacgggca gcgggcgcgg gccgacgagg ctcatctccc cgcgcagcac gttcaggagc
      901 tgcggcagct cgtctatgct gaacttccgc agccagcggc caaaggcgaa gacgcgcggg
      961 tcgttggcga tcttgaaggc ggggccgcgc atttcattct gaaactccag ctcggcgcgg
     1021 cgggcctcgg cgtccgggtg catggtgcgg aatttccaca tcatgaaggg ccgcccgtag
     1081 tgcccggcgc gctcctggcg gaaaaagacg gggccgccgg gcgaggcaag acggatgccg
     1141 atgacggcgg ccagccagag cggcagggtg agcacgagca gcagggctgc cacaacgcgg
     1201 tcggcgaggt ctttgaacag cagcgcccag gacacctgcg gggtgctgtg aaagacgagc
     1261 atgagcttcc cgccaagaac atcgaaggtg gggcgggaga tggcggtctg gaaaaagtcg
     1321 gcggcgatcc atgcctccac gccctcggtc tcgcaggcct gcacggcttc ctcgattttc
     1381 ccaaaatgca catgctgcgc ggcgaagagc acgcgcgcca cggagtgctc gtgcatggcg
     1441 gcgacgaggt cgctgatcgg ctggcgggtg atgtccacgc acccggccac ctcgatgtgc
     1501 gcgcgctgct cggcgggcac cttttccatg agctggcgca tgtcgtcccc ggccccggcc
     1561 agcagcacgc gctcccggcc tttgccgctg gcgaggcggc ggcgcaggcg cgcgcgctgc
     1621 acctcctccc gcagcagcag ggcggagccg gacatgacga tggcgagcat ggccaccgag
     1681 cggctgggca cctcccactt taaaaacacc accagcgctc ccacggccag gctgatgccg
     1741 gcgagcgcct ccatgagctg gcgcaggctc tgcgcggtgg ttttctccac cagtccggag
     1801 taaaagccgc gctcctccag gatgagcggc gtgaagggca ccaccagggc catcagccag
     1861 tagagctgct cgatgggcgg gatctcgacc agatcgggga tgaaaacagg cagtgtgcgc
     1921 gagcggaagt aatgcgcgaa ccacaggcaa aaggccagca gcccgctgtc gagaagctgg
     1981 gtaagctgca aattgatttc ttgccggcgg ccaagcatag tgttcggatt ccgatgtgtt
     2041 tcgaattctt gaaattatag ctgtcttaag taatgccaca acttctttcc tcctcaaacc
     2101 tcctgctttc ctcccgcccg ctcaccgtcg cggtcatccc ggacgcggcg acgctggcgc
     2161 tgtggcaggg aatggagccg gaggcgcgcg cggcggcgtg cgatgtcatc gagctgcggc
     2221 tggacacgct gcagaccgcg ccgggggaca tccgggcctc tctccagggc aatgccacgc
     2281 ccgttttgct caccgcgcgc caccccgccg agggcggcca gggcagcgcg g
//
LOCUS       DAOMYJ010000442         2319 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Prosthecobacter sp. isolate AMR_MDS_5629
            NODE_17717_length_2319_cov_18.235866, whole genome shotgun
            sequence.
ACCESSION   DAOMYJ010000442 DAOMYJ010000000
VERSION     DAOMYJ010000442.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867850
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prosthecobacter sp. (wastewater metagenome)
  ORGANISM  Prosthecobacter sp.
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Verrucomicrobiaceae; Prosthecobacter.
REFERENCE   1  (bases 1 to 2319)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2319)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.30
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:34:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,742
            CDSs (total)                      :: 4,684
            Genes (coding)                    :: 4,676
            CDSs (with protein)               :: 4,676
            Genes (RNA)                       :: 58
            tRNAs                             :: 55
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2319
                     /organism="Prosthecobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17717_length_2319_cov_18.235866"
                     /isolate="AMR_MDS_5629"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1965333"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1170)
                     /locus_tag="PLP58_22925"
     CDS             complement(<1..1170)
                     /locus_tag="PLP58_22925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009962498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1501 domain-containing protein"
                     /protein_id="HRJ11450.1"
                     /translation="MHASRRTFLQSTGCGFGWLAASAMAGGSSTFARLQPHVTPRAKR
                     VIFLFMQGGVSHVDSFDYKPRLIRDHEKTLDLADPRTVAKTGKGAPQNLMKPLWDYAR
                     HGQTGRWASGLFPHMNRHVDDMCFLHGMHTEGVAHGPATLFLHTGTTSFIRPSMGAWV
                     MYGLGSENENLPGFVTISPSLGNGGPRNYGNAFLPAVYQGTPIGRSGIPAADSGIKNI
                     ANAAWTPEQQRRQYELLGALNARQARPGDTEIEAVIQSYELAWRMQQRAPDALDLARE
                     SEATLKLYGIGEKATDNYGRQCLMARRLAEQGVRYIQVNYGDNSNNPAWDQHSNMPKH
                     ADHAAAVDKPVAGLLADLKQRGLLEDTLVWWGGEFGRTPYSQSNGTGRDHNPGGFT"
     gene            complement(1286..1639)
                     /locus_tag="PLP58_22930"
     CDS             complement(1286..1639)
                     /locus_tag="PLP58_22930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ11451.1"
                     /translation="MQRQHPQAANKKPDHGTSLPVQTKAELPGTAFGSAKPGIVFIQT
                     AAKSMIPAAKSICLKAVCMKMTAESILPAIKGIKTAAKGIKTAIKGILLKAKGIWLIV
                     NQILLASIFTLQAIN"
     gene            complement(1608..2036)
                     /locus_tag="PLP58_22935"
     CDS             complement(1608..2036)
                     /locus_tag="PLP58_22935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ11452.1"
                     /translation="MTTIAKYSLPEQAHLARIKLEGQGIKAHVADENVIQWYWYLSNA
                     IGGVRLLVAPEDEAAARACLASELDLSDIELDEEAGCFLCPACGSREVAPDPDAKSFF
                     FVTALIGIPLPLLWPRWKCLACDWRGKESALAETAPASGE"
     gene            complement(2069..>2319)
                     /locus_tag="PLP58_22940"
     CDS             complement(2069..>2319)
                     /locus_tag="PLP58_22940"
                     /inference="COORDINATES: protein motif:HMM:NF024776.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HRJ11453.1"
                     /translation="PPASQPQPAQAESAAPPRAELDVHEMERQLIMQAVERCGGNRSD
                     AAALLNISRRTLQRKLKEMGMVRKHRPRAPRGGAAAAG"
BASE COUNT          366 a          671 c          811 g          471 t
ORIGIN      
        1 ggtgaagccg ccggggttgt ggtcgcgccc ggtgccgttg ctctgcgagt agggcgtgcg
       61 gccgaattcg ccgccccacc agacgagggt gtcctccagc aggccgcgct gtttgaggtc
      121 ggcgagcagg ccggcgacgg gcttgtccac ggcggcggcg tggtcggcgt gcttgggcat
      181 gttgctgtgc tggtcccagg cggggttgtt ggagttgtcg ccgtagttga cctggatgta
      241 acgcacgccc tgctcggcta ggcggcgggc catgaggcac tggcggccgt agttgtcggt
      301 ggctttttcg ccgatgccgt agagcttcaa agtggcctcg gactcccgcg cgaggtcgag
      361 cgcgtccggc gcgcgctgct gcatgcgcca ggccagctcg tagctctgga tgacggcctc
      421 gatctcggtg tcgccggggc gcgcctggcg ggcgttgagc gcgccgagca gctcgtactg
      481 ccgccgctgc tgctccggcg tccaggccgc gttggcgatg tttttgatgc cggagtccgc
      541 cgccgggatg ccgctgcggc cgatgggcgt gccctggtaa acggcgggca ggaaggcgtt
      601 gccgtaattg cgcgggccgc cgttgccgag gctggggctg atggtgacaa atccgggcag
      661 gttttcattc tccgaaccca gcccgtacat gacccacgcg cccatgctgg ggcggatgaa
      721 gctggtggtg ccggtgtgga ggaaaagggt ggcaggcccg tgcgccacgc cctcggtgtg
      781 catgccgtgg aggaagcaca tgtcgtccac atgccggttc atgtgcggga aaaggccgga
      841 ggcccagcgc ccggtctggc cgtgccgcgc gtagtcccac agcggcttca tgagattctg
      901 cggggcgccc ttgccggtct tggcgacggt gcgcgggtcg gcgagatcga gcgtcttttc
      961 atggtcgcga atgagccgcg gcttgtaatc aaaggagtcc acatggctca ccccgccctg
     1021 catgaagaga aaaatgacac gcttcgccct cggtgtgacg tgcggctgca ggcgcgcgaa
     1081 cgtggatgaa ccgccggcca ttgcggaggc tgccagccag ccaaaaccgc agccggtgga
     1141 ttgcagaaaa gtgcgtcggg aagcgtgcat gagggcttaa acgctcactc agtggggcgt
     1201 ttttgcctgc gttgggcaag ttttgcacaa aaacccggcg agcgatgggc atgccgggca
     1261 gttgttatga caagtatgaa tgacgctaat taattgcctg aagtgtgaaa attgaggcaa
     1321 ggagtatctg gttaacgatc aaccagatac cttttgcctt caaaaggata cctttgatgg
     1381 ctgttttgat acctttggcc gctgtcttga tacctttgat ggccggaagg atgctttcgg
     1441 cggtcatctt catacagaca gccttcaaac agatgctttt ggcggccgga atcatgcttt
     1501 ttgccgctgt ttggatgaag acgatgcccg gcttggcgct tccgaaggcc gttcctggca
     1561 gttcggcttt cgtttggacc ggcaggctgg ttccatgatc tggtttttta ttcgccgctt
     1621 gcgggtgctg tctctgcaag ggcggactct ttgccgcgcc agtcgcaggc caggcatttc
     1681 cagcgtggcc agagcagggg caggggaatg ccaatgaggg cggtgacaaa gaaaaagctt
     1741 ttggcatctg gatcgggcgc cacttccctg gagccgcagg ctgggcagag aaagcagccc
     1801 gcttcctcgt ccagctcgat gtcggagagg tccaactcgg aagccaggca ggcgcgcgcc
     1861 gccgcttcat cctccggggc caccagcagc ctgacaccgc cgatggcgtt ggagaggtac
     1921 caataccact gaatcacatt ttcatcggcc acatgcgcct tgatgccctg gccctccagc
     1981 ttgatgcgcg ccagatgggc ctgctctggg agggagtatt tggcgatggt ggtcatgagg
     2041 gatccgttgt gaacgcgccg cgcacgtctt acccggcggc agcggctcca ccacgcggcg
     2101 cgcgggggcg gtgtttgcgc accatgccca tctccttgag cttccgctgg agggtgcggc
     2161 ggctgatgtt gagcagggcg gcggcatcgc tgcggttgcc gccgcagcgc tccacagctt
     2221 gcatgatgag ctgcctctcc atttcatgaa cgtccagttc agccctgggc ggcgcggcgc
     2281 tctccgcctg cgccggctgg ggctgcgagg cgggcggca
//
LOCUS       DAOMYJ010000443         2318 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Prosthecobacter sp. isolate AMR_MDS_5629
            NODE_17736_length_2318_cov_20.718073, whole genome shotgun
            sequence.
ACCESSION   DAOMYJ010000443 DAOMYJ010000000
VERSION     DAOMYJ010000443.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867850
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prosthecobacter sp. (wastewater metagenome)
  ORGANISM  Prosthecobacter sp.
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Verrucomicrobiaceae; Prosthecobacter.
REFERENCE   1  (bases 1 to 2318)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2318)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.30
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:34:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,742
            CDSs (total)                      :: 4,684
            Genes (coding)                    :: 4,676
            CDSs (with protein)               :: 4,676
            Genes (RNA)                       :: 58
            tRNAs                             :: 55
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2318
                     /organism="Prosthecobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17736_length_2318_cov_20.718073"
                     /isolate="AMR_MDS_5629"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1965333"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2318)
                     /locus_tag="PLP58_22945"
     CDS             complement(<1..>2318)
                     /locus_tag="PLP58_22945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ11454.1"
                     /translation="GQLPLAANAAVVDWSFFQAPWPRGFEDLAIGPDLRESFRQWRNS
                     PREKPVHPWFLRRALPQDATAEDRARAELLTQAAASGLTLFGHEEDLDPLGRPKLSLT
                     EFQLAATSGRSAPVSVAIIQSSLLWSRLTDSAYCAAYFPGLIPTRGAPRSLADSFNRF
                     AGTGNAGGTQNGRAALLQMLVNVAESAQPDIVPPVIDRSRGIVGARSMPYVAEVTTRA
                     RSALWLLPEDVRNDTARLLETDSSGRYAFKHPYQGVDRHLRFYATSVVLDLCIGFINP
                     NPFETGDFQGEIEVEVDWGSLSSGAFVQRGWLKAPISGRYTPTPEPGKDPKKMHALGQ
                     CARIELGVVPASFLNDDNRASVLRIKGWRILRGGQVWHEVPVPAPGQSTARPWWEMTR
                     SGNNLGSPEDEYSLEAYRAGGYRAAGWFTKATVESLIPDTLFVSPSWTSDTAPVSEEV
                     RSSVEFWERHTRKAAVLERLASLDPVLGHRTGSNSPAGLFGRGHFYGALGHTWRHRPF
                     GENTPAVPPEMPEGQEPVLETKSVTARPWQADFAGTGSQSVRVRQPVGGKLVTRSQNV
                     PVPQWSTSATGPLLGGEYLTQKYLEPDPNGGDGTEEPPPEDEPDERHDPDLPGSLTGI
                     YLCPAVDTHAPQGMTAFVDYTKVKFTVTSGTLAATFKDMDVETSGAGQIPLVDEQFTK
                     RTDGKKGPRSLLCSAPAKRPCASVGELGFIHSGFPQTPILTGPDEGRTEFQLNSPRNG
                     PPMRMMLDILAPPRYTDARGAPVSEGEWLAGSA"
BASE COUNT          348 a          644 c          933 g          393 t
ORIGIN      
        1 gcgctgccgg cgagccactc gccctcgctg acgggggcgc cgcgcgcgtc ggtgtagcgg
       61 ggcggggcga ggatgtcgag catcatgcgc atgggcgggc cgttgcgcgg gctgttgagc
      121 tggaattcgg tgcggccctc gtccgggccg gtgaggatgg gtgtctgggg gaagccggag
      181 tgaatgaagc cgagttcgcc tacggaggcg caggggcgct tggcgggtgc gctgcacagg
      241 agggagcgag ggcctttttt gccgtcggtg cgcttggtga actgctcgtc cacaaggggg
      301 atctggcctg ctccggaggt ctccacgtcc atgtccttga aggtggctgc cagagtgcca
      361 ctggtgacgg tgaatttgac tttggtgtaa tccacaaaag cggtcattcc ctggggggcg
      421 tgggtgtcca cggcggggca gaggtagatg ccggtgaggg agccgggcag gtcgggatca
      481 tgccgctcgt cgggctcgtc ctcgggagga ggctcttcgg tgccgtcgcc gccattaggg
      541 tcgggctcga ggtatttttg agtgaggtat tcgccgccga ggagcgggcc ggtggcgctg
      601 gtgctccatt gggggacggg cacgttctgg ctgcgggtga cgagcttccc gcccacgggc
      661 tggcgcacgc gcacactctg gctgccggtg cctgcaaaat cggcctgcca ggggcgggcg
      721 gtgacgcttt tggtctccaa cacgggttcc tggccttccg gcatctctgg cggaacggcg
      781 ggggtattct ccccgaaagg ccggtgccgc caggtgtggc cgagcgcgcc gtagaagtgg
      841 ccgcgcccga agagaccggc gggggagttt gagccggtgc ggtggccgag cacggggtcg
      901 aggctggcga ggcgctcgag gacggcggct ttgcgcgtgt ggcgctccca gaactcgacg
      961 ctgctccgca cctcctcgct gacgggggcg gtgtcggagg tccaactggg ggatacaaaa
     1021 agtgtgtcgg gaatgaggct ttccacggtg gctttggtga accacccggc ggcgcggtag
     1081 ccgccagcgc ggtaggcttc gagggaatac tcgtcctcgg ggctgccgag attgttgccg
     1141 ctgcgggtca tctcccacca ggggcgggcg gtgctctggc cgggcgcggg cacggggacc
     1201 tcatgccaga cctggccgcc gcgcaggatg cgccaacctt tgatgcgcag gacgctggcg
     1261 cgattgtcgt cgttgaggaa ggaggcaggc acgacgccca gctcaatgcg ggcgcactgg
     1321 ccgagggcgt gcattttttt ggggtccttg cccggctcgg gcgtgggagt gtagcggccg
     1381 gagatggggg ctttgagcca gccgcgctga acgaaggcac cgctggagag cgatccccag
     1441 tccacttcga cttcaatttc gccctggaag tcccctgtct cgaaggggtt cggattgatg
     1501 aagccgatgc agagatcaag gacgacgctg gtggcgtaga agcgcaggtg gcggtccacg
     1561 ccctggtagg gatgcttgaa agcgtagcgc ccggagctgt cggtctccag caagcgggca
     1621 gtgtcattgc gcacatcctc cggcagcaac caaagggcgc tgcgggcgcg ggtggtgacc
     1681 tcggcgacgt agggcatgga gcgcgcgccg acgatgccgc gactgcggtc tatgacgggc
     1741 gggacgatgt cgggctgcgc ggactcggcg acattgacga gcatctggag gagggcggcg
     1801 cgaccgttct gggtgcctcc tgcattgccg gtgccggcga agcggttaaa ggagtcggcc
     1861 aatgagcgcg gggctccacg cgtgggaatg aggccgggga aataggcggc gcagtaagcg
     1921 gagtcggtga ggcgggacca gagcaggctg ctttgtatga tggcgacgga gactggcgcg
     1981 gagcgtccgc tggtggcggc gagctggaat tcggtgaggc tgagctttgg cctgcccagc
     2041 gggtcgaggt cctcctcatg gccgaagagg gtgaggccgg aggcggcggc ctgggtgagc
     2101 agctcagcgc gggcgcgatc ctcggcggtg gcatcttgcg gcagggcgcg gcgcaggaac
     2161 caagggtgga cgggtttttc acgcgggctg ttgcgccatt ggcggaagct ctcgcgcaaa
     2221 tcggggccga tggcgagatc ctcaaaaccg cgcggccagg gcgcctggaa aaaagaccag
     2281 tccaccacgg cggcattggc ggcgagcggg agctggcc
//
LOCUS       DAOMYJ010000444         2313 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Prosthecobacter sp. isolate AMR_MDS_5629
            NODE_17787_length_2313_cov_15.920283, whole genome shotgun
            sequence.
ACCESSION   DAOMYJ010000444 DAOMYJ010000000
VERSION     DAOMYJ010000444.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867850
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prosthecobacter sp. (wastewater metagenome)
  ORGANISM  Prosthecobacter sp.
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Verrucomicrobiaceae; Prosthecobacter.
REFERENCE   1  (bases 1 to 2313)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2313)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.30
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:34:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,742
            CDSs (total)                      :: 4,684
            Genes (coding)                    :: 4,676
            CDSs (with protein)               :: 4,676
            Genes (RNA)                       :: 58
            tRNAs                             :: 55
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2313
                     /organism="Prosthecobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17787_length_2313_cov_15.920283"
                     /isolate="AMR_MDS_5629"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1965333"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..400
                     /locus_tag="PLP58_22950"
     CDS             <1..400
                     /locus_tag="PLP58_22950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ11455.1"
                     /translation="LPRLRRGALSLTPGLAAKIQPLPDSSAEAAAIGVIEWRIESLAA
                     GAYEVILHYACPALDSVLPVTLTLGKQSLATRLDSGKTTPDAATYSILRLGRLDLPAE
                     ARGGILSLSAGEPARLHLKQLILTPVKTTD"
     gene            428..1153
                     /locus_tag="PLP58_22955"
     CDS             428..1153
                     /locus_tag="PLP58_22955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009964260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HRJ11456.1"
                     /translation="MGAQWKQKWRELAADQKGKIVGKLTREIQVAARLGGPDPDLNAR
                     LHAAIAAARKQSVTRDAIERAIAKGSGIGGEPVNYQTVVFEGFAPHRVPVMVECLTDN
                     NNRTSSEVRVLFKAGSMGTPGSVAWMFEHCGLIEAHHPDAAQDIETAAIEAGADNVEP
                     LELDDEDTAGHAGARFLTATTDLDAVTKALKAAGWQITTSELHHQPKDCPPLDDTQRA
                     EVTAFLQALDGHADVHRVYAAMK"
     gene            1243..2121
                     /gene="mtnP"
                     /locus_tag="PLP58_22960"
     CDS             1243..2121
                     /gene="mtnP"
                     /locus_tag="PLP58_22960"
                     /EC_number="2.4.2.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018971158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017061 - S-methyl-5-thioadenosine
                     phosphorylase activity [Evidence IEA];
                     GO_process: GO:0006168 - adenine salvage [Evidence IEA];
                     GO_process: GO:0019509 - L-methionine salvage from
                     methylthioadenosine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-methyl-5'-thioadenosine phosphorylase"
                     /protein_id="HRJ11457.1"
                     /translation="MADTLPPAIGIIGGSGLYEIEGFEQPEELEITTPFGPPSDKLVG
                     GVLAGRRVYFLPRHGRGHRILPTELPHRANLWALRSLGVRHVIAVTAVGSLRAEYHPR
                     DVLLPDQFFDRTSRRAEHTFFGRGIVAHVAFADPLSAGLRALLKQAAAEQGARVHDGG
                     TYVCMDGPAFSTRAESNAHRQLGFDVIGMTNLPEAKLAREAEMALATLAMITDFDCWK
                     TDEEHVTAEAVMGHVQANAAMAKAILACVIPRIPNDACWPEHRSLDGSLMTPRQLWPE
                     STTQELRPLLERFLEG"
BASE COUNT          404 a          864 c          719 g          326 t
ORIGIN      
        1 cctgccgcgc ctgcgccggg gcgcgctcag tctcacgccc ggactcgccg ccaagatcca
       61 gcccctgccc gactccagcg ccgaggccgc cgccatcggc gtcatcgagt ggcgcatcga
      121 aagcctcgcc gccggagcct acgaggtcat cctgcactac gcctgcccgg cgctggactc
      181 cgtgctgccg gtcacactca ccctggggaa gcagagcctt gccacacggc tcgactccgg
      241 caagaccacc ccggacgccg ccacctacag catcctccgc ctgggccggc tcgacctgcc
      301 cgccgaggcg cgcggcggca tcctcagcct gagcgccggc gagccggccc gcctgcatct
      361 caagcagctc atcctcaccc ctgtcaaaac cacggactga accccaaacc ggaccctcaa
      421 acatctcatg ggagcacaat ggaaacagaa atggcgcgaa ctggccgcgg atcaaaaagg
      481 caaaatcgtc ggcaagctga cgcgtgaaat ccaggtcgcc gcccgcctcg gcggccccga
      541 ccccgacctc aacgcgcgcc tccacgccgc catcgccgcc gcgcgcaagc aaagcgtcac
      601 gcgcgacgcc atcgagcgcg ccatcgccaa aggcagcggc atcggcggcg agccggtgaa
      661 ctaccagacc gtcgtcttcg agggcttcgc accgcaccgc gtgccggtga tggtcgagtg
      721 cctcaccgac aacaacaacc gcacctcctc cgaagtccgc gtgcttttca aagcgggcag
      781 catgggcacg cccggctccg tcgcctggat gttcgagcac tgcggcctca tcgaggccca
      841 ccatccggac gccgcccagg acatcgaaac cgccgccatc gaagccggcg cggacaacgt
      901 cgagccgctc gaactcgacg atgaggacac cgccgggcat gccggcgcgc gctttctcac
      961 cgccaccacc gatcttgacg ccgtcaccaa ggcgctcaag gccgccggct ggcagatcac
     1021 cacttcggaa ctccaccacc agcccaagga ctgcccgccg ctcgacgaca cccagcgcgc
     1081 cgaggtcacc gccttcctcc aggctctcga tggccacgcc gacgtccacc gcgtctatgc
     1141 cgccatgaaa tgagagtgcc ggggcgcggc ctgggcgccg caaccatgac gcgatgacac
     1201 cgcgcccttg cgcacggcgg gccgcgcctt atggatgggc gcatggcaga cacactccct
     1261 ccagcgatcg gcatcatcgg cggctccggg ctttatgaaa tcgaaggctt cgagcagccg
     1321 gaggagctgg agatcaccac gcccttcggc ccgccatcgg acaagctggt gggcggcgtc
     1381 ctcgccggtc ggcgcgttta tttcctgccc cggcacgggc gcggacaccg catcctgccc
     1441 acggagctgc cgcaccgcgc caatctctgg gctctgcgct cgctgggcgt gcgccacgtc
     1501 atcgccgtca ccgccgtggg cagtctgcgg gcggaatacc acccgcggga tgtgctgctg
     1561 ccggaccagt tttttgaccg caccagccgg cgcgctgagc acaccttctt cgggcgcggc
     1621 atcgtggcgc atgtggcctt tgcggacccg ctcagcgcgg gcctgcgcgc gctgctgaag
     1681 caggctgccg cggagcaggg cgcgcgcgtt catgacggcg gcacttatgt gtgcatggac
     1741 gggccggcct tctccaccag agcggagtcg aacgcgcacc ggcagctcgg ctttgacgtg
     1801 atcggcatga ccaatctgcc ggaggccaag ctggctcgcg aggccgagat ggcgctggcg
     1861 acgctggcga tgatcaccga cttcgactgc tggaaaacgg acgaggagca cgtcaccgcc
     1921 gaggcggtga tgggccatgt tcaggccaat gcggcgatgg cgaaagccat cctcgcatgc
     1981 gtcatcccgc gcatcccgaa tgatgcctgc tggccggagc accgctcgct cgacggctcc
     2041 ctcatgacgc cgcgccagct ctggccggag agcacgacgc aggagctgcg gcccctgctg
     2101 gagcgcttcc tcgaagggtg atgcctcgca cgcctcaatg cgcggagttt attcgatgtc
     2161 ttccgccgcg gaaatgaccg cgacttcgga gtcaccttcc gccatttcag ccggaacggc
     2221 ggcaggctcg ccgaggagat ggcgccgcag aaaagcgagc gcgtcgccga gcaggccatc
     2281 gggatagcct gtgccgtcaa aatgctcgtg atg
//
LOCUS       DAOMYJ010000445         2296 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Prosthecobacter sp. isolate AMR_MDS_5629
            NODE_17970_length_2296_cov_15.526551, whole genome shotgun
            sequence.
ACCESSION   DAOMYJ010000445 DAOMYJ010000000
VERSION     DAOMYJ010000445.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867850
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prosthecobacter sp. (wastewater metagenome)
  ORGANISM  Prosthecobacter sp.
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Verrucomicrobiaceae; Prosthecobacter.
REFERENCE   1  (bases 1 to 2296)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2296)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.30
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:34:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,742
            CDSs (total)                      :: 4,684
            Genes (coding)                    :: 4,676
            CDSs (with protein)               :: 4,676
            Genes (RNA)                       :: 58
            tRNAs                             :: 55
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2296
                     /organism="Prosthecobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17970_length_2296_cov_15.526551"
                     /isolate="AMR_MDS_5629"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1965333"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..738)
                     /locus_tag="PLP58_22965"
     CDS             complement(<1..738)
                     /locus_tag="PLP58_22965"
                     /inference="COORDINATES: protein motif:HMM:NF024600.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EF-hand domain-containing protein"
                     /protein_id="HRJ11458.1"
                     /translation="MKTTLLCALGLLAAGVSTASAQQGGGRLAEFLKRADKDGDGKIS
                     KEEFKSLSQRDDGDERFSRMDTNGDGFVDQSEMAAIGERMRRGPGGPPESPGSKSEGG
                     FRRPPSENPEGKPAPEGERPPGPPGERPPGDRPQGDRPPGGPGMGPNPEEIYGRMDKN
                     SDGFVDKDEYVAFAREEIEQRFGRLDENADGKISKEEMKAGMERLRNAMRGGQGGPPG
                     MPGGEGMRRPGGGPPGEGGFRRPEGGPP"
     gene            874..1263
                     /locus_tag="PLP58_22970"
     CDS             874..1263
                     /locus_tag="PLP58_22970"
                     /inference="COORDINATES: protein motif:HMM:NF019498.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HRJ11459.1"
                     /translation="MNTPPSKTQGPLLIRHEGHTPRERSTCGWRDRLISREDAPLHPA
                     AWAHAVDIDGARPHYHKRSTELYYVLDGGGEVVLDGVAHPVMKGSLVHIPPGVVHGAR
                     GKMRVLVVGVPDIAEDDYFEPEETSAA"
     gene            1317..>2296
                     /locus_tag="PLP58_22975"
     CDS             1317..>2296
                     /locus_tag="PLP58_22975"
                     /inference="COORDINATES: protein motif:HMM:NF024777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LamG domain-containing protein"
                     /protein_id="HRJ11460.1"
                     /translation="MRLCFFLILPPLLHAEYQETWETGYSGRDADGAHVLGYWKFDEG
                     AELKDSSGKGNDLALNGALTVAAGKNGGALESFEGIPVEDKPHQARVTAASRLTPAGA
                     FTFELWMRPKGEFEKGARCHLADKKYVPDNHADYAWQITEADKGGLRRLLVTLGFGSH
                     SESFYSDPLRIASGEWRHLAFTYDGAGSVAFYHNGSTLSRVTKPGLGPLAPGRRGLFL
                     GDRGGSSYSGFPGFLDEVRLCEGALKFEPVEIEVASARSVWQRMERAEPVQILCTNLR
                     RETLRGASLRVDFDGKVETFLVPDLNPGAAHTARFPVNTALKAGAYTVRAR"
BASE COUNT          366 a          720 c          798 g          412 t
ORIGIN      
        1 gggagggccg ccttcggggc ggcggaatcc gccctcaccg ggaggaccgc cgccgggacg
       61 gcgcatgcct tcaccgccgg gcattccggg agggccgccc tggccgccgc gcatcgcgtt
      121 gcgcaggcgc tccatgcctg ccttcatttc ttctttgctg atcttgccgt cggcgttttc
      181 atccaggcgg ccaaagcgct gttcgatctc ctcgcgggcg aaggcgacat attcgtcctt
      241 gtccacaaag ccgtcactgt ttttgtccat gcggccatag atttcctcgg ggttgggtcc
      301 catgcctggg ccgccggggg gacgatctcc ttgcggacgg tcgccagggg ggcgctcacc
      361 gggagggcct gggggccgtt cgccttcggg cgcgggtttg ccttcgggat tctccgaagg
      421 cgggcggcgg aagccgccct ccgatttgct gccggggctt tcaggaggtc cgccgggtcc
      481 gcgtcgcatg cgttcgccga tggcggccat ctcggactgg tccacaaagc cgtcgccatt
      541 ggtgtccatg cgggaaaagc gctcgtcgcc gtcgtcgcgc tgggagaggg atttgaactc
      601 ctctttgctg atcttgccgt cgccgtcctt gtcggcgcgt ttcaggaact cagccagacg
      661 gccgccgccc tgctgggcgg atgctgtgga gacaccggcg gccagaaggc cgagggcgca
      721 cagaagggtg gttttcaatt tcataaggtg tgtgggtgtg cgggttggta acgcaagccc
      781 tggaacaacc ctccgcgccg ccggttgcgc gggcatgcaa aaaagatgcg cggctttgac
      841 aggagacctg gaaaaggtta ggcgcgccag accatgaaca caccaccctc caagactcaa
      901 ggcccgctgc tcatccgcca tgaggggcac acgccgcgcg agcgcagcac ctgcggatgg
      961 cgcgaccgcc tcatcagccg cgaggacgcg cctctgcacc cggcggcttg ggcgcacgcg
     1021 gtggacattg acggcgcgcg gccgcattac cacaagcgca gcacggagct gtattatgtg
     1081 cttgatggcg gcggcgaggt ggtgctcgac ggcgtggcgc atccggtcat gaaaggctcc
     1141 ctcgtccaca tcccgcccgg cgtggtccac ggggcgcggg gaaaaatgcg cgtgctcgtc
     1201 gtcggcgtgc cggacatcgc cgaggacgac tacttcgagc cggaagaaac ttccgcggcg
     1261 tgagtcattc ggctgaaagg cacgggcgct gagccgggat atactccgcc ccgaccatgc
     1321 gactttgttt ttttctcatc ctccccccgc tgttgcacgc ggagtaccaa gaaacctggg
     1381 aaaccggcta ctccggcagg gacgcggatg gcgcgcatgt gctgggttat tggaagttcg
     1441 acgagggcgc ggaactcaag gacagctccg gcaagggcaa cgacctggct ttgaacgggg
     1501 ctctgacggt ggctgcgggc aaaaatggcg gcgcgctgga atccttcgag ggcatcccgg
     1561 tggaggacaa gccgcatcag gcgcgggtga ccgccgccag ccggctcacg cccgcgggcg
     1621 cgttcacatt cgagctgtgg atgaggccga agggggagtt cgagaaaggc gcgcgctgcc
     1681 atctggcgga caagaagtat gtgccggaca accacgccga ttacgcctgg cagatcaccg
     1741 aggcggacaa gggcggcctg cgccgcctgc tggtcacgct tggcttcggc tcgcattcgg
     1801 agtcgtttta ttcggacccg ctgcgcatcg cttccggcga atggcggcat ctggccttca
     1861 cttatgacgg cgcgggttcg gtggcgtttt atcacaacgg ctccacgctc agccgcgtca
     1921 ccaagcccgg actcggcccg ctggcgccgg gcaggagagg gcttttcctg ggcgaccgcg
     1981 gcggcagcag ttattcgggt ttccccggtt ttctcgatga agtccggctc tgcgagggag
     2041 cgttgaaatt cgagccggtc gaaatcgagg tggccagcgc gcgctcggtg tggcagcgca
     2101 tggagcgcgc ggagccggtt cagatcctct gcacgaacct gcgccgcgag acactgcgcg
     2161 gcgcgagcct gcgggtggat ttcgacggca aggtggagac ttttctcgtt ccggatttaa
     2221 atccaggcgc cgcgcacacg gcgcgctttc cagtgaacac cgcgctgaag gctggagcct
     2281 acaccgtgcg cgcgcg
//
LOCUS       DAOMYJ010000446         2288 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Prosthecobacter sp. isolate AMR_MDS_5629
            NODE_18071_length_2288_cov_19.315271, whole genome shotgun
            sequence.
ACCESSION   DAOMYJ010000446 DAOMYJ010000000
VERSION     DAOMYJ010000446.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867850
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prosthecobacter sp. (wastewater metagenome)
  ORGANISM  Prosthecobacter sp.
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Verrucomicrobiaceae; Prosthecobacter.
REFERENCE   1  (bases 1 to 2288)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2288)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.30
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:34:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,742
            CDSs (total)                      :: 4,684
            Genes (coding)                    :: 4,676
            CDSs (with protein)               :: 4,676
            Genes (RNA)                       :: 58
            tRNAs                             :: 55
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2288
                     /organism="Prosthecobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18071_length_2288_cov_19.315271"
                     /isolate="AMR_MDS_5629"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1965333"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..854)
                     /locus_tag="PLP58_22980"
     CDS             complement(<1..854)
                     /locus_tag="PLP58_22980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ11461.1"
                     /translation="MTRLLPALLAVFLCLPAPAQENPKAKKKGARAAQSAPSFTAPLA
                     QHLKLADAALHWDCPDFVIGADLRLWAVAVEHDGAADTLRLLSRVDGGWQQVAALSEP
                     GVVHQPALAADGARGVWAVWSQVDGREVMTLRARRHKGDDLEDEVVLAAPEGAGCGFA
                     DAGTDAAGRVWVVWQEIRPGASQVWARHWSREGGAWSAPIRISAEEKGGHWEPRVAFT
                     DTEGAWVVYDSSAGAEFNLRLARAGLDGTVRHLELPSTPEYEARASVAADGAGGLWIT
                     AERGRRSM"
     gene            complement(1159..>2288)
                     /locus_tag="PLP58_22985"
     CDS             complement(1159..>2288)
                     /locus_tag="PLP58_22985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ11462.1"
                     /translation="GIIRDDAGRAILNYLRKFYQHGWAFREVMRHAPTLCLPDDHDVF
                     QGNIWGEGGKPMDTGDGGASSKGGYIQPARMVNVVHKTNCAHHPDAYDAKPVLQDISV
                     YYGDMVYGGVSFAILADRQWKSGPERVDTGSGRADHVLAADFDTAKLDKPGLILLGER
                     QEAFLKEWASDWRGHKLKAVLSQTVFANVATHHGGFDGYLKADLDSGGWPQTPRNRTI
                     ELMRPSMALHINGDQHLTTLVQYGVDRQRDSNWSFCTPAIAAGYPRWWRPDDLRMPHA
                     NRPKHGLPDTGEYIEGLGNKAYVYAVGNPQVGQAPKRYDKAHEKGSGFGFITFDTDKL
                     VYHVESFRFLIDATDGKPGNQFPGWPVTIQQKENRGENLLQ"
BASE COUNT          336 a          808 c          775 g          369 t
ORIGIN      
        1 ctcatactcc ggcggccacg ctccgccgtg atccacaggc ccccggcccc gtccgccgcc
       61 acactggcgc gcgcctcata ctccggcgtg gacggcagct ccagatggcg caccgtgccg
      121 tccaaccccg cacgggccag gcgcaggttg aattccgccc ctgccgagct gtcatagacc
      181 acccaggcgc cctccgtgtc ggtgaatgcc acgcgcggct cccagtggcc gcctttttcc
      241 tccgccgaga tccggatggg ggccgaccac gcaccgccct cccggctcca gtgccgcgcc
      301 cacacctgcg aggcgcccgg ccggatctcc tgccacacca cccacacccg gcccgccgca
      361 tccgtgcccg cgtcggcgaa gccgcagcct gccccctccg gcgcggccag caccacctca
      421 tcctccagat catcaccctt gtggcggcgc gcgcgcagcg tcatcacctc ccgcccgtcc
      481 acctggctcc acaccgccca caccccgcgc gcgccatccg ccgccagcgc cggctggtgg
      541 acaacccccg gctcggaaag cgccgccacc tgctgccacc cgccgtccac cctggacaga
      601 aggcgcagcg tgtccgccgc cccgtcgtgc tccaccgcca ccgcccagag gcgcaggtcc
      661 gcgccgatga cgaagtccgg gcagtcccag tgcagggccg catccgccag cttcaaatgc
      721 tgggccaggg gtgcggtgaa gctgggcgct gactgcgcgg ccctggcccc tttcttttta
      781 gcctttgggt tctcctgggc gggcgcaggc agacacagga aaacggcgag cagggcgggc
      841 aagaggcgcg tcatgccccg gggaacgccg ccgcccactc aggcttgcgg gaaagtcacc
      901 cgccgatgca gggaggcgca ggccaagccc ccgcccgccg cgcggggagg ctgcgggtgc
      961 gcatgacgct gacggccagt tcggatatgc gcccaccgca cgcggcgctg aggggcggga
     1021 ttgacctgac tgaccgcatg acacgcctgc ggcggatgga tgccctgccg ccgggcttgg
     1081 cgggcagcgg ggcgtcaatg cggtcagcag tggcagcaaa gtccccccca gcccgccgcg
     1141 cgcggaggct gcggggtgtc actggaggag gttctccccg cggttctcct tttgctggat
     1201 cgtcaccggc cagccgggga attgattgcc aggcttgccg tcggtggcgt caatcaggaa
     1261 gcggaaggac tcgacatgat aaaccagctt gtccgtgtcg aaggtgatga agccgaagcc
     1321 gctgcccttt tcatgggcct tgtcatagcg cttcggcgcc tggccgactt gcgggttccc
     1381 gacggcatag acgtaggcct tgttgccgag gccctcgatg tattcgcccg tgtcgggcag
     1441 gccgtgcttc ggccggttgg cgtggggcat tctcagatcg tcaggccgcc accagcgcgg
     1501 gtaaccggcg gcgatggcgg gggtgcagaa ggaccagttg ctgtcccgct gcctgtccac
     1561 gccgtactgc acgagcgtgg tcaaatgctg gtcgccattg atgtgcaggg ccatgctggg
     1621 acgcatcagc tcgatggtgc ggttgcgcgg cgtctgcggc cagccgccgg agtcgaggtc
     1681 ggccttcaga taaccgtcaa agccgccgtg atgagtggcg acgttggcga agacggtctg
     1741 gctgagcacg gctttgagtt tatggccgcg ccagtcgctg gcccactctt tgaggaacgc
     1801 ttcctggcgc tcgccgagca ggattaggcc gggcttgtcg agcttggcgg tgtcgaagtc
     1861 cgccgccagg acatggtcgg cacggccgct gccggtgtcc acgcgctcgg ggccgctctt
     1921 ccactggcgg tcggcgagga tggcgaagct gacgccgcca tagaccatgt cgccataata
     1981 cacactgatg tcctgcagca cgggcttggc gtcgtaagcg tccgggtggt gggcgcagtt
     2041 ggttttatgc acgacgttga ccatgcgcgc cggctggatg tagccgccct ttgaggaggc
     2101 gccgccgtcg ccggtgtcca tgggtttgcc gccctcgccc cagatgttgc cctggaagac
     2161 atcgtgatcg tcgggcaggc agagcgtggg cgcgtggcgc atgacctcgc ggaaagccca
     2221 gccgtgctgg taaaacttgc gcaggtagtt gaggatggcg cgcccggcat cgtcgcggat
     2281 gatgccga
//
LOCUS       DAOMYJ010000447         2274 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Prosthecobacter sp. isolate AMR_MDS_5629
            NODE_18224_length_2274_cov_17.646688, whole genome shotgun
            sequence.
ACCESSION   DAOMYJ010000447 DAOMYJ010000000
VERSION     DAOMYJ010000447.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867850
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prosthecobacter sp. (wastewater metagenome)
  ORGANISM  Prosthecobacter sp.
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Verrucomicrobiaceae; Prosthecobacter.
REFERENCE   1  (bases 1 to 2274)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2274)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.30
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:34:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,742
            CDSs (total)                      :: 4,684
            Genes (coding)                    :: 4,676
            CDSs (with protein)               :: 4,676
            Genes (RNA)                       :: 58
            tRNAs                             :: 55
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2274
                     /organism="Prosthecobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18224_length_2274_cov_17.646688"
                     /isolate="AMR_MDS_5629"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1965333"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..592)
                     /locus_tag="PLP58_22990"
     CDS             complement(<1..592)
                     /locus_tag="PLP58_22990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ11463.1"
                     /translation="MPLEAAEREQIITDALAAINQPGAAPDIARQVMEGIGQRPGLSD
                     TDKQKICDDVRKQWVAQQTPALLAGANGPGGTVQACQNVLQSPDYRAGDKAQLVGALV
                     HGRMDNAVQQAVAANNASDDKQPEQAVRSAAVKAEIMAIIQDIEDAPGLSADQKRDMQ
                     ADVLAGCIGLDKDPSVEIKLGGEIFAAIEDSDALTPD"
     gene            complement(659..2152)
                     /locus_tag="PLP58_22995"
     CDS             complement(659..2152)
                     /locus_tag="PLP58_22995"
                     /inference="COORDINATES: protein motif:HMM:NF021897.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretion system DNA-binding
                     domain-containing protein"
                     /protein_id="HRJ11464.1"
                     /translation="MFSFLKFLVAVGIAGSGVWYMLQYDSINEVTGGVAFVCLLVAMV
                     LIKWQPDLSKKDYIVQYKNLKWLPGEFTRHWLITGDTGVGKTTSGFNPLLHQISVSRP
                     NWGGLILGAKGDEHFFAIEHFTGHGRPEAVCVLAVRPDRLDRTWQPKERYNLVSDKRL
                     PYTTHAKNLVDTGASLTEGEQSSFFKPAAQQALTSAFELLESLGKPVNVLRAYDFLTD
                     KSTMEQLFNELLDADATPERIKLAKYFDQTFLSAQAAEQKEGLVGTLKVMLGFFTHPD
                     IAEIFCSDEPNTIDIQDVDKGRVFCTAMPQTFQTERRYINTYIKLLFYTHAMMRFDKP
                     KSARKGENLLIALMDEFQALATASEDGISDHNVIDRLRAANCCLILGMQSDASLFPVL
                     GKETAQVLTLNCRSRIAFRQPDPEGAEAVAQFIAKVEKKKVSKSSGFMGANSSKTVNK
                     EWDYEVQPGELMKMPDATAWIVHPSKKKIKMRIPAMDGAGKIPDWWK"
BASE COUNT          352 a          707 c          755 g          460 t
ORIGIN      
        1 ggtccggggt gagcgcgtcg ctgtcctcga tggccgcgaa gatttccccg cccagcttga
       61 tttcgacgct gggatccttg tccaaaccga tgcaacccgc cagcacgtcc gcctgcatgt
      121 cgcgcttttg gtcggcggac aatccggggg catcttcgat gtcctgaatg atggccatga
      181 tctcagcctt caccgcggcg ctcctcaccg cctgctcggg ctgcttgtca tcactggcgt
      241 tgttcgccgc cacggcctgc tggaccgcgt tgtccatcct gccatgcacc agcgcgccca
      301 cgagctgcgc cttgtccccg gctctgtaat cgggggattg caggacgttt tgacaggcct
      361 gcacggttcc gccaggcccg ttcgcgccgg cgagcagggc gggcgtctgc tgcgccaccc
      421 actgcttgcg cacatcgtcg cagatctttt gtttgtcggt gtcggaaagg ccgggccgct
      481 gcccgattcc ctccatgact tgtcttgcga tgtccggcgc cgcgccgggc tggttgatcg
      541 cggcaagggc gtcggtgatg atttgttcgc gttcggctgc ttcgagtggc atggagtttg
      601 ggtgtggagt tgggtgtgga ggcttgggga agggcatgcc ggcgaaaagg acggatcatc
      661 acttccacca gtccgggatc ttgcccgcgc cgtccatggc ggggatgcgc atcttgatct
      721 tcttcttgct ggggtggacg atccaggcgg tggcgtcggg catcttcatc agctcgccgg
      781 gctgcacctc gtagtcccac tctttgttga ccgtcttgct gctgttggcg cccatgaagc
      841 cggaggactt ggagactttc ttcttctcca ctttggcgat gaactgggcc acggcctcgg
      901 cgccctcggg gtcgggctgg cggaaggcga tgcgcgagcg gcagttgagc gtgagcacct
      961 gggcggtctc cttgcccagc acggggaaca atgacgcgtc gctctgcatg ccgaggatga
     1021 ggcagcagtt cgcggcgcgc aggcggtcaa tgacattgtg gtcggagatg ccgtcctcgc
     1081 tggcggtggc cagcgcctgg aactcgtcca tgagggcgat gagcaggttc tcccccttgc
     1141 gcgcggactt gggcttgtca aagcgcatca tcgcgtgcgt gtagaagagc agcttgatgt
     1201 aggtgttgat gtagcggcgc tccgtctgga aggtctgcgg catggcggtg cagaagacgc
     1261 ggcccttgtc cacatcctgg atgtctatgg tgttcggctc gtcggagcag aagatttccg
     1321 cgatgtccgg gtgggtgaag aagccgagca tgaccttcag ggtgccgacg aggccctcct
     1381 tctgctccgc ggcctgggcg ctgaggaagg tctggtcgaa gtacttggcc agcttgatgc
     1441 gctccggcgt ggcatccgcg tcgagcagct cgttgaaaag ctgctccatc gtggacttgt
     1501 ccgtcaggaa gtcgtaggcg cgcagcacat tgacgggctt gccgaggctc tccagcagct
     1561 cgaaggcgct ggtcagcgcc tgctgggcgg cgggtttgaa gaaggagctc tgctccccct
     1621 ccgtcagtga ggcgccggtg tccaccaggt tcttcgcgtg cgtcgtgtag ggcaggcgtt
     1681 tgtcggacac cagattgtag cgctccttgg gctgccacgt ccggtcgagc cggtccgggc
     1741 gcaccgccag cacgcacacc gcctccggcc ggccgtggcc ggtgaagtgc tcgatggcga
     1801 agaaatgctc gtcccccttg gcgcccagga tcagcccgcc ccagttgggc cgcgagaccg
     1861 agatctggtg cagcagcgga ttgaagccgg aagtcgtctt gcccacgccc gtgtcccccg
     1921 tgatgagcca gtggcgggtg aactcccccg gcagccactt gaggttcttg tactgtacaa
     1981 tgtagtcttt cttggagagg tccggctgcc atttgatcag caccatcgcc accagcaggc
     2041 agacaaaggc gacgccgccg gtgacctcgt tgatcgaatc atactggagc atgtaccaca
     2101 caccggaacc ggcgatgccc acggcgacga ggaatttgag aaaggagaac atgaaaaaaa
     2161 ctgcggctgc cgtgtcaata gcccgtcccc cgctccttgt gacacagcaa acgcgccccg
     2221 accccgcccg ccaaaagcgc cgcgcggcac ggacccgccc gcgccgcgcg cacc
//
LOCUS       DAOMYJ010000448         2272 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Prosthecobacter sp. isolate AMR_MDS_5629
            NODE_18245_length_2272_cov_17.231394, whole genome shotgun
            sequence.
ACCESSION   DAOMYJ010000448 DAOMYJ010000000
VERSION     DAOMYJ010000448.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867850
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prosthecobacter sp. (wastewater metagenome)
  ORGANISM  Prosthecobacter sp.
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Verrucomicrobiaceae; Prosthecobacter.
REFERENCE   1  (bases 1 to 2272)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2272)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.30
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:34:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,742
            CDSs (total)                      :: 4,684
            Genes (coding)                    :: 4,676
            CDSs (with protein)               :: 4,676
            Genes (RNA)                       :: 58
            tRNAs                             :: 55
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2272
                     /organism="Prosthecobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18245_length_2272_cov_17.231394"
                     /isolate="AMR_MDS_5629"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1965333"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(197..544)
                     /locus_tag="PLP58_23000"
     CDS             complement(197..544)
                     /locus_tag="PLP58_23000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007416213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HRJ11465.1"
                     /translation="MNTKMYVGNLPFSATEDDVRTLFSDYGTVTEVYLPMDRESGRPR
                     GFAFVTMDSQTAMNEAIKGLNDKDYGGRNLAINEARPKEERPAYSGGGGGYGGGGRGG
                     KGGGRGGYGGGRY"
     gene            complement(742..1212)
                     /locus_tag="PLP58_23005"
     CDS             complement(742..1212)
                     /locus_tag="PLP58_23005"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009962249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HRJ11466.1"
                     /translation="MSFRHTLKDRVQFADTDMAGIVHFSNFFRYMERVEHDFFRSLGM
                     SIWDGNNLEVAAEARVGWPRVHASCDYKAPLRFEEEFEMELLVEDIRARTLRYRVRFW
                     KSDGTLAAEGSLVAACVQKDPASGRMKAVSIPDRVRERLSTAPPESLAPPKGHA"
     gene            1320..>2272
                     /locus_tag="PLP58_23010"
     CDS             1320..>2272
                     /locus_tag="PLP58_23010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007416916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HRJ11467.1"
                     /translation="MTADLVGKLRALLAPDRVLMADVDILPYSFDGTAALKQKPGAVV
                     FPLTPEEVAACVGAAREAAVPVVTRGSGTGLSGGSVPVPGCLVVCLARMDRLIEVDEK
                     NLTLRAQCGVITKEIDDAAAKAGLFYPPDPGSMKISTIGGNVAENSGGLRGLKYGVTR
                     DYVMGLQVVLPDGTLATLGNKCVKDVAGYSMKDLFIGSEGTLGIITEVTLKLLPRPRA
                     RRTMLALYDSMEAAAETISAIIAAKIIPCTLEFLDRVTVECVEDYAKIGLPVDVEALV
                     LMETDGHPAVVEEEAARIMDLARQNGAREVRAAADEAEGARL"
BASE COUNT          398 a          707 c          765 g          402 t
ORIGIN      
        1 ggcctggaaa aactcgtggc gcgcggagac gccgccgagt ccctcaccgc cgccgcgcgc
       61 gaggccctca tggcgatggg gctggagccg ccgcagcccg gcaaggtgga ctgaagactc
      121 caagccggac aaaaaacggc atcccgtctc cgggatgccg tcttgaatgg gtcagatgga
      181 tgcgccgcgc gctggatcaa taacgaccgc cgccgtagcc gccacgaccg ccgcctttgc
      241 cgccgcggcc accgccgccg tagcctcctc ctcctccgga gtaagcgggt cgttcttctt
      301 tcgggcgggc ttcattgatg gcgaggttgc gaccaccgta gtctttgtca ttgagaccct
      361 tgatggcctc gttcatggcc gtctgggagt ccatggtgac gaaggcgaag ccgcgcgggc
      421 ggcctgactc gcggtccatg ggcagataga cttcggtgac ggtgccatag tcggagaaga
      481 gagtgcgaac gtcgtcttcg gtggcgctga agggcaggtt gcccacatac attttggtgt
      541 tcatagattt ggatttgtcg cgcaagactg ctcaggctgc gggaagctcc aggtgcggat
      601 ttcagaacac caaagaatca acctgatgat cagcttcatt tagcctttgt caggttaact
      661 tgcgccccct ctgtcgtctg ctttgaggga atggcaaacg atagtttgga aatcttgccg
      721 ggcgtgtccg cgctcgaaaa atcaggcgtg acccttcggc ggcgccagtg actccggcgg
      781 cgcggtgctc agcctctcac gcacgcgatc ggggatggac acagccttca tccggccgct
      841 ggcggggtct ttctggacgc aggcggccac gaggctgcct tcggcggcga gcgtgccgtc
      901 gcttttccaa aagcgcaccc ggtagcgcag cgtcctggcg cggatgtcct cgacgagcag
      961 ctccatctcg aactcctctt caaagcgcag cggcgccttg tagtcgcacg aggcatggac
     1021 acgcggccag ccgacgcggg cctccgccgc cacctccagg ttgttgccgt cccagatgga
     1081 catgcccagg gagcggaaaa aatcatgctc cacgcgctcc atgtatcgga agaaatttga
     1141 aaagtggacg atgccggcca tgtcggtgtc agcgaactgg acgcggtcct tgagagtgtg
     1201 gcggaaagac atgcgccgac tatggagcgc gccggatgca aagcgccagc cttttgtcaa
     1261 agcgggagtg cggagcttgc gcgcggcggg cggcccgcca gactgcgcgc gtgaattccg
     1321 tgaccgccga ccttgttgga aaactccgcg ccctgctcgc tcccgaccgc gtgctgatgg
     1381 cggatgtgga catcctgcct tacagtttcg atggcacggc agcgctcaag cagaagccgg
     1441 gcgcggtcgt ttttcctctg accccggagg aggtggccgc ttgcgtcggc gcggcgcggg
     1501 aggccgccgt gccggtggtg acgcgcggct ccggcacggg cctgagcggt ggcagcgtgc
     1561 cggtgccggg ctgcctggtt gtgtgcctgg cgcgcatgga ccggctcatc gaggtggatg
     1621 agaaaaacct caccctgcgc gcgcagtgcg gcgtcatcac caaggaaatt gacgacgcgg
     1681 cggcaaaggc gggcctgttt tatccgcccg accccggctc gatgaagatc agcaccatcg
     1741 gcggcaatgt ggccgagaac tccggcggac tgcgcggcct caaatacggc gtcacgcgcg
     1801 attatgtgat gggcctgcaa gtggtgctgc ctgacggcac cctcgccacg ctcggcaaca
     1861 agtgtgtcaa agacgtggcc ggctattcga tgaaggacct ctttattggc agcgagggca
     1921 cgctgggcat catcaccgag gtcacgctca agctgctgcc gcgcccgcgg gcgcgccgga
     1981 cgatgctggc gctctatgac agcatggagg ccgccgccga aactatctcc gccatcatcg
     2041 cggcgaagat catcccctgc acgctggagt tcctggatcg cgtgacggtg gaatgcgtgg
     2101 aggactacgc caaaatcggc ctgcctgtgg atgtggaggc gctcgtgctc atggagactg
     2161 acggccaccc cgccgtggtc gaggaggagg ccgcgcgcat catggatctg gcccgccaaa
     2221 acggcgctcg cgaggtgcgc gccgcagccg acgaggccga gggcgcgcgc ct
//
LOCUS       DAOMYJ010000449         2271 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Prosthecobacter sp. isolate AMR_MDS_5629
            NODE_18260_length_2271_cov_20.578520, whole genome shotgun
            sequence.
ACCESSION   DAOMYJ010000449 DAOMYJ010000000
VERSION     DAOMYJ010000449.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867850
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prosthecobacter sp. (wastewater metagenome)
  ORGANISM  Prosthecobacter sp.
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Verrucomicrobiaceae; Prosthecobacter.
REFERENCE   1  (bases 1 to 2271)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2271)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.30
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:34:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,742
            CDSs (total)                      :: 4,684
            Genes (coding)                    :: 4,676
            CDSs (with protein)               :: 4,676
            Genes (RNA)                       :: 58
            tRNAs                             :: 55
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2271
                     /organism="Prosthecobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18260_length_2271_cov_20.578520"
                     /isolate="AMR_MDS_5629"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1965333"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            75..707
                     /gene="pth"
                     /locus_tag="PLP58_23015"
     CDS             75..707
                     /gene="pth"
                     /locus_tag="PLP58_23015"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018970257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HRJ11468.1"
                     /translation="MTGDATAPATTEAQTPRLIVGLGNPGPEYRNTRHNAGFMVLDEL
                     AGRMGASFAEESRFHGLVAKFSGGWLLKPRTFMNDSGRSVAALARFHKISPAETLVVH
                     DEVDLALGALKFRQSGSAAGHNGIKSLIQHLGTQAFPRLKVGIGSATGRPAGERLAGH
                     VLGGFRPEEWVEMQISIQRAADAVMRALHSGLAAAMNLFNQKPSTPQTPQ"
     gene            704..994
                     /locus_tag="PLP58_23020"
     CDS             704..994
                     /locus_tag="PLP58_23020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009958576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HRJ11469.1"
                     /translation="MKRKYEALIVLDMKGKEETVEDLVNGIGREMEKAGAKLEEIDRL
                     GKRQFPHNPRHVESGYFVNFKIEADNAALDSMRAKFKLNENVYQQYYQRREE"
     gene            complement(1122..>2271)
                     /locus_tag="PLP58_23025"
     CDS             complement(1122..>2271)
                     /locus_tag="PLP58_23025"
                     /inference="COORDINATES: protein motif:HMM:TIGR00368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="YifB family Mg chelatase-like AAA ATPase"
                     /protein_id="HRJ11470.1"
                     /translation="ARGRRRILVPKRAAAEASVVAGIDIIGVATLQEVVNFLKGDLDI
                     PPEPCRAAEYFAASAHYDMDFADVKGQRDAKRAIEVAVAGGHNILMVGPPGTGKSMIA
                     KRIGTIMPGMTEEEAIETTKIHSAAGLLGESNAFVATRPFRPPHHTISDAGLLGGGSN
                     PGPGEVSLAHNGVLFLDELPEFRRSTLEVMRQPLEDGKVTISRAAGTVTFPAQFMLVA
                     AMNPCQCGFYGDRKRECRCSPPMIQKYRQRISGPLLDRIDLHVEVPLVDYKALANAEP
                     GESSAEIRARVERARAVQAERFRGQKDVHTNSHMTPRLIKKHCELDAEGAAILEHQMG
                     TMSFSARAHDRILKVARTLADLKGLEKIDSDSVLEAVGYRTLDRNLWA"
BASE COUNT          410 a          761 c          715 g          385 t
ORIGIN      
        1 tgccgccgcg ccggcacccg ccgctgccgc gaagaagtaa tcccggacag gcagcctgag
       61 acagcgcccc agcagtgacc ggagatgcca cagcacccgc caccaccgag gcgcagacac
      121 cgaggctgat cgtcggattg ggaaatccag gaccggaata ccgcaacacc cgtcacaacg
      181 caggcttcat ggtcttggat gaactggctg gtagaatggg cgcctctttc gcggaggaga
      241 gccgctttca cggcctcgtc gccaaattct ccggcggctg gctgctcaag ccccggacct
      301 tcatgaacga cagcggccgc agcgtggcgg cgctggcgcg tttccacaaa atcagccccg
      361 ccgagacgct cgtcgtccac gacgaggtgg acctggccct tggcgctttg aaattccgcc
      421 agtccggctc cgccgcgggt cacaacggca tcaagtccct catccagcat ctaggcaccc
      481 aggcattccc gcgcctcaaa gtcggcatcg gctccgccac cggcaggccg gcgggggagc
      541 gccttgccgg gcatgtcctc ggcggcttcc gcccggagga gtgggtggaa atgcaaatct
      601 ccatccagcg cgccgccgat gccgtcatgc gcgccttgca ttccggcctt gccgccgcca
      661 tgaatctttt caaccagaaa cccagcacac cccagacacc ccaatgaagc gcaaatacga
      721 agccttgatc gtcctcgaca tgaaaggcaa agaagaaacc gtggaagacc tcgtcaacgg
      781 catcggccgc gaaatggaaa aggccggagc caaactcgag gagattgacc gcctcggcaa
      841 acgccagttc ccgcacaatc cccgccatgt cgagagcggc tacttcgtga acttcaagat
      901 cgaggctgac aatgcggccc tcgacagcat gcgcgcgaag ttcaagctca acgaaaacgt
      961 ttaccagcag tactaccagc gccgggaaga gtaggccgga ccgcgctcgc gcgcagcctc
     1021 cggcaatgcg ccaggaattt cgaggagatg cccgcctgtg gcggtcgtct cctcttttta
     1081 tcagctgact ggcgcgtccg cccgcacccg catcaagaaa gtcaggccca aagattgcgg
     1141 tccagcgtgc ggtagcccac ggcttcgagg acgctgtcgg agtctatttt ttccaggcct
     1201 ttcaaatccg cgagagtgcg ggccaccttc agaatgcggt catgagcgcg ggcgctgaag
     1261 ctcatcgtgc ccatctggtg ctccaggatg gcggcgccct cggcatccag ctcgcagtgt
     1321 tttttgatca ggcgcggagt catgtggctg ttggtatgca cgtccttctg gccgcgaaaa
     1381 cgctccgcct gcacggcgcg tgcccgctcc acccgggcgc ggatttcggc tgaggactcc
     1441 cccggctcgg cgtttgccag cgccttgtag tccaccagcg gcacctcgac atgcaggtct
     1501 atgcggtcga gcaaagggcc gctgatgcgc tggcggtatt tttggatcat cggcgggctg
     1561 caccggcact cgcgcttgcg gtcgccataa aagccgcact ggcaggggtt cattgccgcc
     1621 accagcatga actgcgcggg gaaggtgacg gtgcccgccg cgcgcgagat ggtgactttt
     1681 ccatcctcca gcggctggcg catgacctcc agggtgctgc ggcggaactc gggcagctcg
     1741 tcgagaaaca gcacgccatt atgcgccagg ctcacctcgc cagggccggg attgctgccg
     1801 ccgccgagca gcccggcatc gctgatcgtg tggtgcggcg ggcggaaggg gcgggtggcg
     1861 acgaaggcgt tcgactcgcc cagcaggccg gcggcgctgt ggattttggt ggtctcaatg
     1921 gcctcctcct cggtcatccc cggcatgatg gtgccgatgc gcttggcgat catgcttttg
     1981 cccgtgccgg gcgggccgac catgaggatg ttgtgcccgc cggccacggc cacctcgatg
     2041 gcgcgcttgg catcccgctg gcctttcaca tcggcgaagt ccatgtcgta atgcgccgac
     2101 gcggcgaaat actcggcggc gcggcagggt tcgggcggga tgtccagatc gcccttcaag
     2161 aagttcacca cctcctgcag cgtggccacg ccgatgatgt caatgccggc caccacgctg
     2221 gcctcggcgg cggcgcgctt gggcacgaga atgcgtcgcc ggccgcgcgc c
//
LOCUS       DAOMYJ010000450         2235 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Prosthecobacter sp. isolate AMR_MDS_5629
            NODE_18696_length_2235_cov_18.102752, whole genome shotgun
            sequence.
ACCESSION   DAOMYJ010000450 DAOMYJ010000000
VERSION     DAOMYJ010000450.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867850
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prosthecobacter sp. (wastewater metagenome)
  ORGANISM  Prosthecobacter sp.
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Verrucomicrobiaceae; Prosthecobacter.
REFERENCE   1  (bases 1 to 2235)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2235)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.30
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:34:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,742
            CDSs (total)                      :: 4,684
            Genes (coding)                    :: 4,676
            CDSs (with protein)               :: 4,676
            Genes (RNA)                       :: 58
            tRNAs                             :: 55
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2235
                     /organism="Prosthecobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18696_length_2235_cov_18.102752"
                     /isolate="AMR_MDS_5629"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1965333"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1127
                     /locus_tag="PLP58_23030"
     CDS             <1..1127
                     /locus_tag="PLP58_23030"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HRJ11471.1"
                     /translation="LRYGRGHGAPATAAGRRPIVVWNITRRCNLKCLHCYQDSDSRHY
                     PGELDWQQCQSVVDDLAQFKVPALLLSGGEPMIHPRFFELAEYAAGKGLRLTLSTNGT
                     LIDAENARRLKDIGFSYVGISLDGMGATHDHFRGRSGAFEKAVAAFRHCKAVGQKVGL
                     RLTLSSHNIDDLDNILDFIEREDIERVCFYHLVYSGRGAELVEVPHEKTRAAIDKILD
                     RTARWVAEGSPREVLTVDQPVDGPYVYMRLLRENPERAERAWELLNWNGGGANSSGTG
                     ISNIDTQGNVHPDQFWHSLTLGNVKDRPFSEIWTNRDHPELTALRNRKEHLTGRCASC
                     RWLNLCGGGFRVRALQMTGDFWAPDPSCYLRDEETALEAV"
     gene            complement(1188..1943)
                     /locus_tag="PLP58_23035"
     CDS             complement(1188..1943)
                     /locus_tag="PLP58_23035"
                     /inference="COORDINATES: protein motif:HMM:NF016430.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor"
                     /protein_id="HRJ11472.1"
                     /translation="MPENSENSFPTTSQTLLTEMLHPAANVSKPALERFCELYYLPVY
                     GVMRRQGMMVEDAWDQTQIFFARFVRMGLLKSFDPAEGKFRSWLTTCAQNQARSAIKA
                     RSREKRGGGVIFETYDPEQAEREWQAEQTAHLNPDQEFDLQLARNLWRGLKRTLIQSK
                     GDAGGKAALTQALLPLVLLEKWPKQPFPTQEEMAASFGTTTIRLRAYFNHTLKKKARR
                     VFEEEVVRYSPGISTEDVEYLWGLLAQYGEAPH"
BASE COUNT          385 a          762 c          684 g          404 t
ORIGIN      
        1 acctgcgtta cggccgcggc cacggcgcgc ccgccactgc cgccgggcgc cggcccatcg
       61 tggtctggaa catcacccgc cgctgcaacc tcaagtgcct gcactgctac caggactccg
      121 actcccgcca ctaccccggc gaactggact ggcagcaatg ccagagcgtg gtggatgacc
      181 tggcgcagtt caaagtgccc gcgctgctgc tctccggcgg cgagcccatg atccacccgc
      241 gctttttcga gctggccgaa tacgccgccg gcaagggcct gcggctgacg ctttccacca
      301 atggcacgct cattgacgcg gagaatgcgc gccggctcaa ggacatcggc ttctcctatg
      361 tgggcatctc cctggatggc atgggcgcca cgcacgacca cttccgcggg cgcagtggcg
      421 cgtttgaaaa agccgtggcc gccttccgcc attgcaaggc cgtcgggcaa aaagtcgggc
      481 tgcgcctcac cctctccagc cacaacattg acgacctgga caacatcctc gacttcatcg
      541 agcgcgagga cattgagcgc gtctgctttt accacctcgt ctatagcgga cggggagcgg
      601 aactcgtgga ggtcccgcat gaaaagaccc gcgcggccat tgacaaaatc ctcgaccgca
      661 ccgcccgctg ggtggccgag gggagtccgc gcgaggtgct caccgtggat caaccggttg
      721 acggtcccta tgtctacatg cgcctgctgc gtgagaaccc ggagagggcg gaacgcgcct
      781 gggagctgct gaactggaac ggcggcggtg ccaacagctc cggcaccggc atcagcaaca
      841 ttgacaccca gggcaatgtc caccccgacc agttctggca cagcctcacc ctcggcaacg
      901 tcaaagaccg ccccttcagc gagatctgga cgaaccgcga ccacccggag ctgaccgccc
      961 tgcgcaaccg caaggagcac ctcaccggcc gctgcgcctc ctgccgctgg ctgaacctct
     1021 gcggcggcgg cttccgcgtg cgcgccctcc agatgacggg cgacttttgg gcgcctgatc
     1081 cttcctgtta tttgcgcgat gaggagaccg ccctggaggc ggtgtgacag cctccacgcg
     1141 cagcgcaccg atggcgcatt ccgccagcct gtcttgcggg cggggagtta gtggggcgcc
     1201 tcgccatatt gggcgagcaa cccccagaga tattccacat cctcggtgga aatgccgggc
     1261 gagtaacgaa ccacctcctc ctcaaagacg cgccgggctt ttttcttgag cgtgtggttg
     1321 aagtaggccc tgagccggat cgtcgtggtg ccaaagctgg ccgccatttc ctcctgggtg
     1381 gggaaaggct gtttcggcca tttttcaagg agcaccaatg gcagcagggc ctgggtgagc
     1441 gcggctttgc cccccgcatc cccctttgat tggatgagcg tccgcttcag accgcgccag
     1501 agattgcgcg ccagttgcag gtcaaactcc tggtcgggat tgaggtgggc ggtctgctcg
     1561 gcctgccatt cccgctcggc ttgttcggga tcataggttt caaatatgac gccaccgccg
     1621 cgcttctctc tgctccgggc cttgatggcg ctgcgcgcct ggttttgcgc gcaggtcgtc
     1681 agccagcttc ggaatttgcc ctccgccgga tcgaatgact tcaacaagcc catccgcacg
     1741 aaccgggcga agaagatctg tgtctgatcc caggcgtcct ccaccatcat gccctgcctg
     1801 cgcatgaccc cgtaaacggg cagatagtac aactcacaaa accgctccag ggcaggtttg
     1861 ctcacatttg ccgcaggatg cagcatctcc gtcagcaagg tctgcgaggt ggtggggaag
     1921 ctgttctcgg agttctcggg catcagcctt ggataggaga gcgcatccag ccccgcgcaa
     1981 gcaggccttg ccaccctccc cgctttcagg catccgccgc ctcaggagtt gcttgccgcg
     2041 cgggccggtc ccggcttata accccgcgcg cccacccgag tcatgaatca aatcctcgcc
     2101 gaagtcctcg cctcgtttgg ttggcaggag cgtttcgagc ctctcgagcg cgtcggccag
     2161 ggcggcatgg ggcaggtgtg gcgcgcgcgc gaggcgggtt ccggccgggc cgtggccttg
     2221 aaaatcctca gcccc
//
LOCUS       DAOMYJ010000451         2214 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Prosthecobacter sp. isolate AMR_MDS_5629
            NODE_18959_length_2214_cov_21.397406, whole genome shotgun
            sequence.
ACCESSION   DAOMYJ010000451 DAOMYJ010000000
VERSION     DAOMYJ010000451.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867850
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prosthecobacter sp. (wastewater metagenome)
  ORGANISM  Prosthecobacter sp.
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Verrucomicrobiaceae; Prosthecobacter.
REFERENCE   1  (bases 1 to 2214)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2214)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.30
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:34:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,742
            CDSs (total)                      :: 4,684
            Genes (coding)                    :: 4,676
            CDSs (with protein)               :: 4,676
            Genes (RNA)                       :: 58
            tRNAs                             :: 55
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2214
                     /organism="Prosthecobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18959_length_2214_cov_21.397406"
                     /isolate="AMR_MDS_5629"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1965333"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..924)
                     /locus_tag="PLP58_23040"
     CDS             complement(<1..924)
                     /locus_tag="PLP58_23040"
                     /inference="COORDINATES: protein motif:HMM:NF025720.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4407 domain-containing protein"
                     /protein_id="HRJ11473.1"
                     /translation="MNLLDRLERVFFWLSGASTDNLEACPAWERRKYVAFGATVLVPC
                     TFAIIACAYALSTLTDNWLVIAPVSLVWAFIILTVDRALLATYRAYQNIFRKLSQFAL
                     RIVVAMLMGVTIAHPLTLLLFKDTIVSAIEEDRQAEIEQTRQAAAAQKALIEARVAPL
                     EQQIARQREAWNASFQASFLDADGKLIEQPPTEEELKARADREKQISEATAAARERLA
                     ALDADLAKQGAEHQKITAELNHWQTEFEREVNGQRSGIIGLGPRAKSIREDQLVWRRT
                     ESARLTSALDSLTAARAATLAEIKTTEENVNS"
     gene            complement(1037..1582)
                     /locus_tag="PLP58_23045"
     CDS             complement(1037..1582)
                     /locus_tag="PLP58_23045"
                     /inference="COORDINATES: protein motif:HMM:NF036614.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5362 family protein"
                     /protein_id="HRJ11474.1"
                     /translation="MENNPYQTPSANLFGSSSATTPEAVPGEAITQLQRTKPWLRFFG
                     VLMWIAVAFMLLGALAMLVMGVGGAAMGGDAATGLGLPEAGIMIGVAALYVVMAFFYI
                     YPAIKIWKTGTAIKKLMASRQPEDLVAALDHQRAFWKFVGIMLIIMIVLYIGMIVAMG
                     VGVAGAMGGMKALEGVTPPGQ"
     gene            1680..>2214
                     /locus_tag="PLP58_23050"
     CDS             1680..>2214
                     /locus_tag="PLP58_23050"
                     /inference="COORDINATES: protein motif:HMM:NF018028.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HRJ11475.1"
                     /translation="MPETADTLQPVELADGVEIHLRVAGPAARSMAWVVDFLIFAVAL
                     LVIGLALMFASAGIGGRTAIGVFLLCGFILSWFYNVIFEMGRHAATPGQRMLGLKVCG
                     VSGAPVRLPQSVIRNLLRVIDFMPGFYLLGLVCCLFNRRFQRLGDLVADTVVVHVEPK
                     HEPALVEVRAEPVPPPRP"
BASE COUNT          345 a          580 c          832 g          457 t
ORIGIN      
        1 ggagttgacg ttttcctcgg tggtcttgat ctcggcgagg gtggcggcgc gggctgcggt
       61 gaggctgtcg agggcgctgg tgaggcgggc ggactcggtg cggcgccaga cgagctggtc
      121 ttcgcggatg cttttggcac gggggccgag gccgatgatg ccgctgcgct ggccgttgac
      181 ttcgcgctcg aactcagtct gccagtggtt gagttcggcg gtgatttttt ggtgctcggc
      241 accctgcttg gcgaggtcgg cgtcaagggc ggcaagcctc tcacgggcgg cggcggtggc
      301 ttcgctgatt tgtttttcgc ggtcggcgcg ggctttgagt tcctcctcgg tgggcggttg
      361 ctcgatgagt ttgccgtcgg cgtcgaggaa gctggcctgg aaggaggcgt tccaggcttc
      421 gcgctggcgg gcgatctgct gctccaaggg tgcgacgcgg gcttcgatga gggctttctg
      481 cgcggcggcg gcctggcgtg tctgctcgat ctcggcctgg cggtcctcct cgatggcgga
      541 gacgatggtg tctttgaaga gcagcagtgt cagcggatgg gcgatggtga cgcccatgag
      601 catggcgacg acgatgcgca gtgcgaactg ggagagcttg cggaagatgt tttgataggc
      661 tcggtaggtg gccagcaggg cgcggtccac ggtgaggatg atgaaggccc agacgaggga
      721 gacgggggcg atgacgagcc agttgtcagt gagggtggaa agggcgtagg cgcaggcgat
      781 gatggcgaag gtgcatggca cgagtacggt ggcgccgaag gccacatact tgcggcgctc
      841 ccaggcgggg caggcctcaa ggttgtcggt ggaggctccg gagagccaga agaagacgcg
      901 ttccagtcgg tcgaggaggt tcatggcgcg gagggatgag ggtgcgccaa agtgcgggcg
      961 cgcccgcact tgtcaaagat gattcatttt ttgttcgccg ggaggcttca cagatcactg
     1021 ccagcccgct ggtgactcac tggccgggtg gtgtgacgcc ttcgagggcc ttcatgccgc
     1081 ccatggctcc ggccacgccg acccccatgg cgacgatcat gccgatgtag agaacgatca
     1141 tgatgatgag catgatgccg acgaatttcc aaaaggcgcg ttgatgatcc agggcggcga
     1201 cgaggtcttc cggctggcgg ctggccatga gctttttgat ggcggtgccg gtcttccaga
     1261 ttttgatggc cgggtagatg tagaagaagg ccatgaccac atagagggcg gcgacgccaa
     1321 tcatgatgcc tgcctctggc aatcccaagc ctgtggctgc atcgccgccc atggctgcgc
     1381 cgccgactcc catgacaagc atggccaggg ctccaagcag catgaaggcc acggcgatcc
     1441 acatgagcac gccaaagaag cgcagccagg gcttggtgcg ctggagctgg gtgatggcct
     1501 cgccgggcac ggcctcgggg gttgtggcgg aggaggagcc gaacaagttc gcggagggag
     1561 tctggtaggg attgttttcc atgacgggag ggagttggga gtgaattatc gtatttatgc
     1621 tgacagggca gctttttagt tgcaagcggc ggcttttgtc aaaacacatc tccgccttca
     1681 tgcccgaaac cgcagacacc ctccagcccg tggaactcgc cgatggcgtg gagattcacc
     1741 tgcgggtggc tgggcctgcg gcgcgcagca tggcgtgggt ggtggacttt ctcatttttg
     1801 cagtggcact gctggtcatc gggctggcgt tgatgttcgc ctcggcgggc atcggcgggc
     1861 ggacggcgat cggcgtgttt cttttgtgcg ggttcatcct gtcgtggttt tacaatgtga
     1921 tcttcgagat gggccggcat gcggccacgc cggggcagcg catgctgggt ctgaaggtgt
     1981 gcggtgtctc tggcgcgccg gtgcggctgc cgcagtcggt catccgcaat ctgctgcggg
     2041 tgatagactt catgccgggt ttttatcttc tgggactggt ctgctgcctg ttcaaccgcc
     2101 gcttccaaag acttggcgac ctggtggccg acacggtggt ggtgcatgtg gagccgaagc
     2161 acgagcccgc gctggtggag gtgcgcgcgg agcctgtgcc accgccgcgc ccgc
//
LOCUS       DAOMYJ010000452         2183 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Prosthecobacter sp. isolate AMR_MDS_5629
            NODE_19351_length_2183_cov_24.295113, whole genome shotgun
            sequence.
ACCESSION   DAOMYJ010000452 DAOMYJ010000000
VERSION     DAOMYJ010000452.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867850
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prosthecobacter sp. (wastewater metagenome)
  ORGANISM  Prosthecobacter sp.
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Verrucomicrobiaceae; Prosthecobacter.
REFERENCE   1  (bases 1 to 2183)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2183)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.30
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:34:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,742
            CDSs (total)                      :: 4,684
            Genes (coding)                    :: 4,676
            CDSs (with protein)               :: 4,676
            Genes (RNA)                       :: 58
            tRNAs                             :: 55
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2183
                     /organism="Prosthecobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19351_length_2183_cov_24.295113"
                     /isolate="AMR_MDS_5629"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1965333"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..424)
                     /locus_tag="PLP58_23055"
     CDS             complement(<1..424)
                     /locus_tag="PLP58_23055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009958399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HRJ11476.1"
                     /translation="MIRPLPVLAAFLLAPLLPARAAVVEKPVEYKSGDVLCEGWHAHD
                     DALEGKRPAVLVVHQWTGVSDHEKERARMLAELGFNVLVADIYGKGVRPRPPASGQEA
                     GKYKKDRALLRSRLDAALKVLAADPRTDPARIACIGYCF"
     gene            complement(485..1495)
                     /gene="galE"
                     /locus_tag="PLP58_23060"
     CDS             complement(485..1495)
                     /gene="galE"
                     /locus_tag="PLP58_23060"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009961054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003978 - UDP-glucose 4-epimerase activity
                     [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HRJ11477.1"
                     /translation="MAEAKGTLLVTGGAGYIGSHTVARLHEGGERVVVLDNLVFGHRE
                     ALPAAGVTFVKGDMADAGLVERVFAEHKPEAVLHFAAFAYVGESVTDPLKYYRNNLAA
                     PLVLLEAMQRHGCRRFIFSSTCATYGDPVRVPMNEEHPQAPVNPYGASKWMLERVLRD
                     CGTAWGLRSVFLRYFNASGCDPEGRIGEDHDPETHLIPRILMAAKGEIEGLTIFGDDY
                     PTPDGTCVRDYIHVCDLASAHEKALAHLRGGGDTTAVNLGTGRGFSVKEILRAAEQVT
                     GVSIPVTYGPRRAGDPAELVCDPSRAKALLGWEAEHKDPRGHIESAWRWMTGPRGGRY
                     GA"
     gene            1611..>2183
                     /locus_tag="PLP58_23065"
     CDS             1611..>2183
                     /locus_tag="PLP58_23065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009961053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HRJ11478.1"
                     /translation="MQNVRLGIVGLGNMGRAHLHNIRAGKVPGLRVTALCESVGTLPE
                     LLEGEHPFTDVSQMIHSGRIDAILICTPHFSHTTIGIEALQNGLHVLVEKPISVHKAD
                     CERLIDAHTDKGKVFAAMFNMRTNATFKKVKDLIDSGELGAVRRIHWEVTNWFRTNYY
                     YATGGWRGTWKGEGGGVLMNQCPHNLDLFQW"
BASE COUNT          362 a          729 c          744 g          348 t
ORIGIN      
        1 cgaagcagta gccgatgcag gcgatgcgcg cggggtcggt gcgcgggtcc gcggcgagga
       61 ctttgagcgc ggcgtcgagg cgggagcgga gcagggcgcg gtcttttttg tatttgcccg
      121 cctcctggcc ggaggctggg ggccgggggc gcacgccttt gccatagatg tcggcgacga
      181 ggacgttgaa gccgagctcc gccagcatgc gcgcgcgctc cttttcatgg tcgctgacgc
      241 cggtccattg atggacgacg agcacggcgg ggcgtttccc ctccagggcg tcatcatggg
      301 cgtgccatcc ctcgcacaaa acatcgccgg atttgtactc gaccggtttt tcgacgacgg
      361 cggcgcgcgc ggggaggagc ggggcgagca gaaatgcggc gaggactggc agcgggcgga
      421 tcatgccgta cttatactca ggggcgggtt tcctggcaag acgcgccttt gccgtcctgc
      481 cgcgtcacgc gccatacctg ccgccgcgcg ggcctgtcat ccagcgccag gcgctctcga
      541 tatggccgcg ggggtccttg tgctccgcct cccagcccag cagggccttt gcccgggacg
      601 gatcgcagac gagttcggcg gggtcgccgg cgcggcgcgg cccgtaagtc acggggatgc
      661 tgacgccggt cacctgctcg gcggcgcgca ggatttcctt cacggagaag ccgcgcccgg
      721 tgccgagatt gacagcggtg gtgtcgccgc cgccgcgcag gtgggcgagg gctttttcat
      781 gggccgaggc gaggtcgcac acatggatgt agtcgcgcac gcaggtgccg tccggcgtgg
      841 ggtagtcgtc gccgaaaatc gtcagtcctt cgatctcgcc tttcgcagcc atgaggatgc
      901 gcgggatgag gtgtgtctcg gggtcgtggt cctcgccgat gcggccttcg ggatcgcagc
      961 cgctggcatt gaagtagcgc aggaaaaccg acctcagccc ccaggccgtg ccgcagtccc
     1021 gcagcacgcg ctcgagcatc cacttcgacg cgccgtaggg gttcacgggc gcctgcgggt
     1081 gctcctcgtt catcggcacg cggacggggt cgccataggt ggcgcaggtg gaggagaaaa
     1141 tgaagcgccg gcagccgtgg cgctgcatgg cctccagcag cacgagcggc gcggcgaggt
     1201 tgttgcggta gtatttgagc gggtcagtga ccgactcgcc cacataagca aaggcggcga
     1261 agtgcagcac agcctcgggc ttgtgctccg caaacacacg ctccaccaaa ccggcgtccg
     1321 ccatgtcgcc cttgacgaac gtcacgcccg ccgcgggcag cgcctcgcgg tggccgaaga
     1381 cgaggttgtc gagcaccacc acgcgctcgc cgccttcatg caggcgcgcc actgtgtgcg
     1441 agccgatgta gcccgcgccg cccgtcacca agagtgtgcc ttttgcttcc gccatgcgcg
     1501 cctttacaca ggcaaagcgc cgcgcgcaaa cgccgcgcgg tgaagaactt gcgcggagcg
     1561 gctcatcccc ggttgaccag ccagcgccgc ccgctagcct gcgctctcct atgcaaaacg
     1621 taagactcgg catcgtcggc ctcggcaaca tgggcagagc ccatctccac aacatccgcg
     1681 cgggcaaggt gcccggactg cgcgtcaccg ccctgtgcga gagcgtcggc accctgcccg
     1741 agcttctgga gggcgagcac cccttcacgg acgtgagcca gatgatccac tccggccgca
     1801 ttgacgccat cctgatctgc acgccgcact tcagccacac cacgatcggc atcgaggcgc
     1861 tgcaaaacgg cctgcatgtg ctggtggaga agcccatctc cgtccacaag gccgactgcg
     1921 agcgcctgat cgacgcgcac acggacaagg gcaaggtctt cgccgccatg ttcaacatgc
     1981 gcaccaacgc aaccttcaag aaggtgaagg acctcattga cagcggcgag ctcggcgcgg
     2041 tgcgccgcat ccactgggag gtcaccaact ggttccgcac caactactac tacgccaccg
     2101 gcggctggcg cggcacctgg aagggcgagg gcggcggcgt gctcatgaac cagtgcccgc
     2161 acaatctgga cctcttccag tgg
//
LOCUS       DAOMYJ010000453         2174 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Prosthecobacter sp. isolate AMR_MDS_5629
            NODE_19484_length_2174_cov_19.882492, whole genome shotgun
            sequence.
ACCESSION   DAOMYJ010000453 DAOMYJ010000000
VERSION     DAOMYJ010000453.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867850
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prosthecobacter sp. (wastewater metagenome)
  ORGANISM  Prosthecobacter sp.
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Verrucomicrobiaceae; Prosthecobacter.
REFERENCE   1  (bases 1 to 2174)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2174)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.30
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:34:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,742
            CDSs (total)                      :: 4,684
            Genes (coding)                    :: 4,676
            CDSs (with protein)               :: 4,676
            Genes (RNA)                       :: 58
            tRNAs                             :: 55
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2174
                     /organism="Prosthecobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19484_length_2174_cov_19.882492"
                     /isolate="AMR_MDS_5629"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1965333"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(17..403)
                     /locus_tag="PLP58_23070"
     CDS             complement(17..403)
                     /locus_tag="PLP58_23070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ11479.1"
                     /translation="MSGKCSFFLLVPCALAILLAPDARSAIVGDDLQQDMNTYDPPRE
                     LRFPQGYEIVEEARSGARAIKTMPAGTANIAAWRDVGGTRYYMTDWSFNRLVKEGIRP
                     NWLRPLGGSPAPAASSPPATTSRCSR"
     gene            complement(551..1813)
                     /locus_tag="PLP58_23075"
     CDS             complement(551..1813)
                     /locus_tag="PLP58_23075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ11480.1"
                     /translation="MRPLLILLLAAAAPACMAADAATAVQNAHDEIWRRFMDKHHVML
                     DFCTPDGSVSLPTPEECRLGKPNALGWWAPIENGAMFNGMYMDALVTRWRVTRDPAAA
                     GKARRLMQGLLFLNSISDVPGFVGRGVSTDGRSHYPMGSNDQTFPWFHGLWLYWRSGI
                     ATEDEKSTILRHLVTTAEAIEALRWQMPAEPPFGVRGGFGGFSFEDAPRLLFLCKMMH
                     QVTGQAKWDAHYRAHLHQSGGKGDTKTRLEWCAHGMRFDDGHPHSWTACNGVCALRSL
                     WEMETDETLRARYHDGLLQSAATAMGSLKTAAQWDNADTSPFEHDWRVMNTQWRPQTT
                     EHEAQALAQEQLRAYGKVSPRRGLETRLVREPVFAAWVVTLAPDRSLLRQRASEIEQV
                     ITAYDYTRLIYSQFFPVEAAWWRLQEAR"
     gene            complement(1906..>2174)
                     /locus_tag="PLP58_23080"
     CDS             complement(1906..>2174)
                     /locus_tag="PLP58_23080"
                     /inference="COORDINATES: protein motif:HMM:NF013308.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070567 - cytidylyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HRJ11481.1"
                     /translation="NWPAWKALPEPSQTRASGWIMETPQVFQHALLVRAYETVMRERL
                     LVTDEVSALQHLGSPPVHLVKNDSPNPKITFPEDLALAAKMLAL"
BASE COUNT          330 a          601 c          850 g          393 t
ORIGIN      
        1 gcaacggttc gcggatctac ctgctacacc tgctggtggt ggcaggcggg ctggaggccg
       61 ccggggcggg gctgccgccg agggggcgga gccagttggg cctgatgcct tctttgacga
      121 ggcggttgaa gctccagtcc gtcatgtaat agcgggtgcc gcccacgtcc cgccaggcgg
      181 cgatgttggc ggtgcccgca ggcatggtct tgatggcgcg ggcgccgctc ctggcttcct
      241 cgacgatctc gtaaccctgc gggaagcgca gctcgcgcgg cgggtcgtag gtgttcatgt
      301 cctgctgcaa gtcatccccg acgatggcgc tccgggcgtc cggggccaga aggatggcaa
      361 gcgcgcaggg gaccagcagg aagaaggagc attttccact catggcgcga gaatgaggga
      421 aagcccgccg cggatcaacg ccattctgcg cgggcgcgct cgatgcgcag tgatggggac
      481 actcttgtct ctcccggcag actgggcagc ctgcccaacc cgaacaggca ggagtctttg
      541 tgccacactt tcagcgcgct tcctggagcc gccaccaggc ggcttcgacg gggaagaatt
      601 gggagtagat aaggcgggtg tagtcgtagg cggtgatgac ttgttcgatc tcggaggcgc
      661 gctggcggag gaggctgcgg tccggggcga gggtgacgac ccaggcggcg aagacaggct
      721 cgcgcacgag ccgtgtctcc aggccgcggc ggggggagac tttgccatag gcgcggagct
      781 gctcctgggc gagcgcctgg gcttcgtgct cggtggtctg gggccgccac tgggtgttca
      841 tgacgcgcca gtcgtgctcg aaggggctgg tgtcggcgtt gtcccactgg gctgcggttt
      901 tgaggctgcc catggcggtg gcggcgctct ggagcaggcc gtcgtggtag cgcgcgcgca
      961 gagtctcgtc ggtctccatc tcccagaggg agcggagggc gcagacaccg ttgcatgccg
     1021 tccaggagtg ggggtggccg tcgtcgaagc gcatgccgtg ggcgcaccat tcgaggcggg
     1081 tctttgtgtc gcctttgccg ccgctctggt gaaggtgggc gcggtaatgg gcgtcccatt
     1141 ttgcctggcc ggtgacctgg tgcatcattt tgcagaggaa gaggagcctg ggcgcgtcct
     1201 cgaagctgaa gccgccgaaa ccgccgcgca cgccgaaggg tggctcggcg ggcatctgcc
     1261 agcgcagggc ctcgatggct tcggcggtgg tcacgaggtg gcggaggatg gtgcttttct
     1321 cgtcttcggt ggcgatgccg ctgcgccagt agagccagag gccgtggaac caggggaagg
     1381 tctggtcgtt cgatcccatg gggtagtggg acctgccgtc ggtgctcacg ccgcggccga
     1441 cgaagcctgg cacgtcgctg atggagttga ggaagagcag gccctgcatg aggcggcggg
     1501 ctttgccggc ggcggcgggg tcgcgggtga cgcgccagcg ggtgacgagg gcgtccatgt
     1561 acatgccgtt gaacatggcg ccgttttcga tgggagccca ccagccgagg gcgttgggct
     1621 tgccgaggcg gcactcctcc ggggtgggca gggagacgct gccgtcgggc gtgcagaagt
     1681 ccaacatgac gtggtgcttg tccatgaagc gccgccagat ctcgtcatgc gcgttttgga
     1741 cggcggtggc ggcatcggcg gccatgcagg cgggagctgc ggcggcgagg aggaggataa
     1801 ggagggggcg catggagggg aaggtttgaa gctcgaaaat tgaaattcga aatcggagtg
     1861 tcgagcttcc ggctttgaac ttcgagcttc gaagttctgc tccggttaca aggcaagcat
     1921 ttttgcggcg agggcgaggt cttcggggaa ggtgatcttg gggttggggc tgtcgttttt
     1981 caccagatgc acgggggggg agccgagatg ctggagggcg gagacttcgt cggtgacaag
     2041 caggcgctcg cgcatgacgg tctcgtaggc gcgcacgagc agggcgtgct ggaagacctg
     2101 gggcgtctcc atgatccagc ccgaagccct cgtctggctc ggctccggca aagccttcca
     2161 ggccggccag ttct
//
LOCUS       DAOMYJ010000454         2130 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Prosthecobacter sp. isolate AMR_MDS_5629
            NODE_20065_length_2130_cov_19.100241, whole genome shotgun
            sequence.
ACCESSION   DAOMYJ010000454 DAOMYJ010000000
VERSION     DAOMYJ010000454.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867850
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prosthecobacter sp. (wastewater metagenome)
  ORGANISM  Prosthecobacter sp.
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Verrucomicrobiaceae; Prosthecobacter.
REFERENCE   1  (bases 1 to 2130)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2130)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.30
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:34:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,742
            CDSs (total)                      :: 4,684
            Genes (coding)                    :: 4,676
            CDSs (with protein)               :: 4,676
            Genes (RNA)                       :: 58
            tRNAs                             :: 55
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2130
                     /organism="Prosthecobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20065_length_2130_cov_19.100241"
                     /isolate="AMR_MDS_5629"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1965333"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            51..1454
                     /locus_tag="PLP58_23085"
     CDS             51..1454
                     /locus_tag="PLP58_23085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ11482.1"
                     /translation="MAAAAVLALAFGIGWFARSTGERTAVAITDSGDDIERDLTGGGV
                     TLQAGRKTLAERFLQAREMMVRELRFAEAGHQFEQIIKSGEARQPTLNWARYNAAICA
                     IVLGKREEAARHMADMAGDTGEGGSLVTAELAAFFKTLGGRLAGLNGGAEDSGSADYG
                     TAPEAVLGRLIEGLAGWHLGDPQTGMEDLRRFSTILEESLTLPEWVPLYAGLLKPYEH
                     DMRLLASVDKLKESASEVEAKEALDLLRETRAKLQTRGRWDEVLEERAKEFRQDLARL
                     RMERQRREMAEAAVLRQKELAQLAEITAALPSLSQGYDFERVTGLLRDIQFQSPEVQA
                     ALEGKLYLYEQARAFLEQWFADVAEKGWQGRFQRLSGAPLEGRVTEASLRQVTISLER
                     GVVTLPLDTLSPETLLDSAQSFAAQVTDSTDYYTRQERIAVFARVAGLHSLSATVAAQ
                     LMEEHRGFRQRWMKVAQ"
     gene            complement(1402..>2130)
                     /locus_tag="PLP58_23090"
     CDS             complement(1402..>2130)
                     /locus_tag="PLP58_23090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ11483.1"
                     /translation="EAQEWIQTLFSSQRRLLPAEALEAWAEAQEWIQTLFSSQRRLLP
                     GVHFIFKTAAGGLYPRLAHERRRLEMAWRVFCAERLPGGLAETLLDAWRAAEDAEALA
                     RVDAAFHLPADESRRSMEGGRLLLERTRWAPHQGALGRYRALREAGAAPGHFIIVWAA
                     VARFFQLGLGSYLTEYIRLEWDTARQSLACGGRALNLEEMAGLASALMRGRSAGLSLL
                     EADEEAAVTAPPSSSAGESRGAPP"
BASE COUNT          375 a          705 c          717 g          333 t
ORIGIN      
        1 gcggtgtgag cgaggttggc agcggcactg tagtttgtgt atggtagttg gtggcggcgg
       61 cggcggtgct ggcgctggct tttggcatcg gctggttcgc gcgcagcacg ggcgagcgca
      121 ccgcagtggc gatcaccgac tctggcgacg acatcgagcg cgatctcacg ggcgggggag
      181 tgactctcca ggccgggcgc aagacgctgg cggagcgttt tctccaggcg cgcgagatga
      241 tggtgcggga gctgaggttc gccgaagccg ggcaccagtt tgagcagatc atcaaatcgg
      301 gcgaggcgcg ccagcccacg ctgaactggg cgcgctacaa tgccgccatc tgcgccatcg
      361 tgctcggcaa gagagaggag gccgcgcgcc acatggctga catggccggg gacaccggcg
      421 aagggggcag cctggtgacg gccgagcttg ccgcgttttt caaaacattg ggtggcaggc
      481 ttgccggcct gaatggcgga gcagaggaca gcggctccgc cgattacggc acggctccgg
      541 aggcggtgct tggccggttg atcgaaggcc tggccggctg gcatcttggc gatccgcaga
      601 caggaatgga agacctgcgc cgtttttcaa ccatcctgga ggaaagcctc accctgcccg
      661 agtgggtgcc gctttacgcc ggtctgttga agccgtacga gcatgacatg aggctgctcg
      721 ccagcgtgga taagctcaag gagtccgcca gcgaggtcga agccaaagag gcgcttgatc
      781 ttcttcgcga gacccgcgcc aagctgcaaa cccggggccg ctgggacgaa gtgctcgaag
      841 aaagggcaaa ggagttccgc caggatctcg ctcgtctgcg catggagagg cagcgccggg
      901 agatggcgga agccgccgtg ctgcgccaga aggagctggc ccagctcgcc gagatcaccg
      961 ccgcgctgcc gtcgctctcg caggggtatg atttcgagcg ggtgaccggc ctgctgaggg
     1021 acatccagtt tcaatccccc gaagtgcagg cggcgctgga aggcaagctc tacctctacg
     1081 agcaggcacg cgcctttctg gagcagtggt tcgccgacgt ggcggagaaa ggctggcagg
     1141 gccggttcca gcgtctctcc ggcgcgccgc tcgaaggccg tgtcaccgaa gccagcctgc
     1201 ggcaggtcac catctcattg gagcgcggcg tggtgaccct gcctctggac actctgtcgc
     1261 ccgagacact gctggactcc gcgcagtcct tcgcggcgca ggtcaccgac agcacggact
     1321 attacacgcg ccaggagcgc atcgccgtgt tcgcgagggt ggccggtctt cacagccttt
     1381 ccgccactgt cgcagcccag ctcatggagg agcaccgcgg ctttcgccag cgctggatga
     1441 aggtggcgca gtgacggccg cctcctcatc cgcctcgagc agagacagcc cggcggagcg
     1501 tccgcgcatc aatgccgagg caagtccggc catttcctcc agattcagcg cgcgtccgcc
     1561 gcaggccagg gactggcgcg ccgtgtccca ctccagccgg atgtattcgg tgaggtagga
     1621 gccgagtccg agctggaaaa aacgcgccac cgccgcccag acgatgatga aatgacccgg
     1681 cgcggctccc gcctcccgca gcgcgcgata cctgcccagc gcaccctggt gcggcgccca
     1741 gcgcgtgcgc tccagcagca gccggccgcc ctccatgctg cgccggctct cgtccgccgg
     1801 cagatggaaa gccgcgtcaa ctctggcaag cgcctccgca tcctccgccg cgcgccaggc
     1861 atccagcaaa gtctccgcca ggccgcccgg caggcgttct gcgcaaaaca cccgccatgc
     1921 catttccagc ctgcggcgct catgcgccag gcgcggatac agcccgccag cagccgtctt
     1981 gaaaataaaa tgcacacccg gcaataaacg ccgctggctg gagaacagcg tctggatcca
     2041 ctcctgcgct tccgcccatg cctccagagc ctccgccggc aataaacgcc gctggctgga
     2101 gaacagcgtc tggatccact cctgcgcttc
//
LOCUS       DAOMYJ010000455         2125 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Prosthecobacter sp. isolate AMR_MDS_5629
            NODE_20125_length_2125_cov_19.573430, whole genome shotgun
            sequence.
ACCESSION   DAOMYJ010000455 DAOMYJ010000000
VERSION     DAOMYJ010000455.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867850
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prosthecobacter sp. (wastewater metagenome)
  ORGANISM  Prosthecobacter sp.
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Verrucomicrobiaceae; Prosthecobacter.
REFERENCE   1  (bases 1 to 2125)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2125)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.30
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:34:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,742
            CDSs (total)                      :: 4,684
            Genes (coding)                    :: 4,676
            CDSs (with protein)               :: 4,676
            Genes (RNA)                       :: 58
            tRNAs                             :: 55
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2125
                     /organism="Prosthecobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20125_length_2125_cov_19.573430"
                     /isolate="AMR_MDS_5629"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1965333"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..278
                     /locus_tag="PLP58_23095"
     CDS             <1..278
                     /locus_tag="PLP58_23095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ11484.1"
                     /translation="TALQALHFLNDETVHTTAEGIVKALLKTPRSNEDHLTRLHRQLF
                     ARAPSTEEQTLLLTHLKTLKKQTPDPAKAWASLTRSLLRANEFLYLD"
     gene            complement(295..1005)
                     /locus_tag="PLP58_23100"
     CDS             complement(295..1005)
                     /locus_tag="PLP58_23100"
                     /inference="COORDINATES: protein motif:HMM:NF020419.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="HRJ11485.1"
                     /translation="MLRPDTLELWQFLENQPALQGFVLVGGSALALRIRHRVSEDLDF
                     AWPGLKLPRARLEALLHLSDGKGFHARRRDDPGALDEFEIAGMDLHDYQQDFLVNERV
                     KLSFFAADEGLVRILSSTGEEAVRVATLDELFASKALVTAQRSKTRDWFDLFVLMTRH
                     GKTLEDMRAVFQKAGMSMQFDTALQRLCSGQPSKHDEGYEHLMENPPSLEEMTRFFRA
                     ERDAYEQRQAAERGKNVR"
     gene            1366..>2125
                     /locus_tag="PLP58_23105"
     CDS             1366..>2125
                     /locus_tag="PLP58_23105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009964665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1501 domain-containing protein"
                     /protein_id="HRJ11486.1"
                     /translation="MLTRRSLLARSPLLFPGVLSELLAADDPLAPRRPHTRAKAKRLI
                     MIYATGGVSHIDTFDPKPLAKGRDGQGPDKLMGNVFGAAPNKNCGTEVSDIFPHLRGV
                     MDEVCLIRSMKASHFDHSEATLGMHTGSPTFARPSMGSWVSYGLGTLNQNLPSFIVIC
                     PHLPYGGTQVYASDFLPAAHQGTRVIPGDRPIANLQTPPAARDLQTLEIDLTRALNQR
                     HLAARPHDTALAARIKSFETAFQMQTAAPEAFDLA"
BASE COUNT          428 a          806 c          498 g          393 t
ORIGIN      
        1 tcaccgccct gcaagccctc catttcctca acgacgaaac cgtccacacc accgctgaag
       61 gcatcgtcaa agccctgctc aaaacaccgc gctccaatga agatcacctc acccgtctcc
      121 accgccagct cttcgcccgc gccccaagca ccgaagaaca aacccttctt ctcacccatc
      181 tgaagaccct taaaaaacaa actccagacc ccgccaaagc ctgggcttcc ctgactcgtt
      241 cgttgctgag ggcgaacgag tttctctatc tcgattgatt accctatgag cgtttcaccg
      301 cacattcttg ccgcgctcgg cggcttgtcg ctgctcgtag gcgtcgcgct ccgcccggaa
      361 gaatcgcgtc atttcctcaa ggctcggcgg attctccatc aaatgctcat agccttcgtc
      421 atgcttcgaa ggctgaccgg agcagagcct ctgcagcgcc gtgtcaaatt gcatgctcat
      481 gccggctttc tgaaaaactg ctctcatgtc ctccaaagtt ttgccatgac gcgtcatcaa
      541 aacaaaaaga tcaaaccagt cgcgagtctt cgatctttgg gccgtcacca gcgctttgct
      601 ggcaaaaagc tcgtccagcg tcgccacacg aacagcttcc tcacccgtgc tggaaaggat
      661 gcggacgagt ccctcatccg cggcgaagaa actcagcttg acccgctcgt tgaccaaaaa
      721 atcctgctgg tagtcatgca ggtccatgcc ggcgatctca aactcatcca gcgcgccggg
      781 atcgtcccgg cgccgggcat ggaatccttt tccatccgac aagtgaagca gcgcctccag
      841 tcttgctcgt ggcagtttca gacccggcca ggcaaagtcc aagtcctcgc tcacccggtg
      901 ccgaatgcgc agtgccagcg cactgccgcc caccagaacg aagccttgca aagccggctg
      961 gttttccaga aactgccaaa gctcaagtgt gtcaggtcgc agcatctcga aaccccgctt
     1021 caatgttccg cagcatccgc tccaccagtg gtgccgcccg ccttacatcg cggccttgct
     1081 cccgcgcaag ttcccgccac tcccctgcca ccttctccaa tccaaaagcg cagcaggcgt
     1141 caaggatcgt gtgaaatctc ggctgcatca atgccgcgcg caaaatcacc tccggcgccg
     1201 cgcccctgcg cgaccacacc aggctttggt gctttgaggc gaactcttgc ggtgtcatgg
     1261 ctcaagatgg cacgagtgta aggggttcca agcgattcta aatcttcctt ccacaatcct
     1321 gtttctcctg aaatcctgtc aatcctgtcc aaaaaaaacg ccgccatgct cacccgccgc
     1381 tccctcctcg cccgcagtcc gctgctgttt cccggcgtgc tctccgagct gctcgccgcg
     1441 gacgatccgc tcgcgccgcg ccgcccgcac acccgcgcca aggccaagcg actgatcatg
     1501 atctacgcca ccggcggcgt ctcccacatt gacaccttcg accccaagcc gcttgccaaa
     1561 ggccgcgacg gacaggggcc ggacaagctc atgggcaatg tcttcggcgc cgcgcccaac
     1621 aaaaactgcg gcaccgaggt cagcgacatc tttccgcatc tgcgcggcgt catggacgaa
     1681 gtctgcctca tccgcagcat gaaggcctcc cacttcgacc acagcgaggc cacgctcggc
     1741 atgcacaccg gctcgcccac tttcgcgcgt cccagcatgg ggtcctgggt cagctacggc
     1801 ctcggcacgc tgaatcaaaa cctgccctct ttcatcgtca tctgccccca cctgccctat
     1861 ggcggcaccc aggtctatgc cagcgacttc ctgcccgccg cgcatcaggg cacccgcgtc
     1921 attcctggcg accgccccat cgccaacctg caaaccccgc ccgccgccag agacctgcaa
     1981 actctcgaga tcgacctcac ccgcgccctc aaccagcgcc acctcgccgc ccgcccgcac
     2041 gacaccgccc tggccgcccg catcaagtcc ttcgagaccg ccttccagat gcaaaccgcc
     2101 gcgcccgagg ccttcgatct cgccc
//
LOCUS       DAOMYJ010000456         2106 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Prosthecobacter sp. isolate AMR_MDS_5629
            NODE_20407_length_2106_cov_25.394442, whole genome shotgun
            sequence.
ACCESSION   DAOMYJ010000456 DAOMYJ010000000
VERSION     DAOMYJ010000456.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867850
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prosthecobacter sp. (wastewater metagenome)
  ORGANISM  Prosthecobacter sp.
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Verrucomicrobiaceae; Prosthecobacter.
REFERENCE   1  (bases 1 to 2106)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2106)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.30
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:34:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,742
            CDSs (total)                      :: 4,684
            Genes (coding)                    :: 4,676
            CDSs (with protein)               :: 4,676
            Genes (RNA)                       :: 58
            tRNAs                             :: 55
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2106
                     /organism="Prosthecobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20407_length_2106_cov_25.394442"
                     /isolate="AMR_MDS_5629"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1965333"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            187..1044
                     /locus_tag="PLP58_23110"
     CDS             187..1044
                     /locus_tag="PLP58_23110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009960981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate hydrolase family protein"
                     /protein_id="HRJ11487.1"
                     /translation="MPNPWTGIGNPYTKQEVIDRLKATLAKGEPIIAAGAGTGISAKF
                     IEKGGADLIIIYNSGRFRMMGHGSTCGLMAYGDANAIAMEIGEYEVLPVVKEIPVICG
                     VHATDPRRRMWHWLGRVKEMGFSGVNNFPTHTIVDGHFRGVLEETGMSVRKEFEMVGL
                     ASRMDLFSIVYVATPEEAVEMAKNGADAVIAHVGTTVGGSIGVVNAVVDWDFTVNRTQ
                     EIIDAAKSVKPDIFTLTHGGPINTPKDVEYILGKTTADGFVGASSLERMGVEDSLTSL
                     TREFKGVRR"
     gene            complement(1053..2027)
                     /gene="queA"
                     /locus_tag="PLP58_23115"
     CDS             complement(1053..2027)
                     /gene="queA"
                     /locus_tag="PLP58_23115"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012419765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HRJ11488.1"
                     /translation="MLTADFDYTLPPELIASEPLAGRADSRMMVVQRDSGVVEHRHFS
                     ELPGFMREGDLLVLNDTRVVPARFFSNDGKKELLRVDSLTATRWRCMVKPGRRLRPGH
                     TVGIGAATGTVVEVLENGDRVIEFDQPVDEDAHGHLALPHYMGRMDRAEDRERYQTVY
                     ARASGAIAAPTAGLHFTPDLLAALPHAFVTLHVGAGTFQPVKAERVEDHVMHSERYQV
                     PEETAAAIAAARRVIAVGTTSMRTLESVTRDHGRITAAGGSTDIFIYPGFKFRACGAL
                     LTNFHLPKSTLLMLVSAFAGKDLILSAYAEAVREKYRFFSYGDCMLIL"
BASE COUNT          405 a          607 c          736 g          358 t
ORIGIN      
        1 ggcgcgcacc acgcgatagt cggtgtccgg gccgtgggtg tgcgtttcgg atagttcatg
       61 gatggcggcg gaggcgtgca tggtggcggg acttgtggtt tacgctgctt gtcgctttat
      121 agcatgtgtc atccggcagt gtgccgcgcg tgaccatcca acatcaatcc caaatccacc
      181 accaccatgc ccaacccctg gaccggaatc ggcaatccct acacgaagca ggaagtcatt
      241 gaccgcctga aagccaccct ggccaagggg gagcccatca ttgccgcagg cgcgggcact
      301 ggcatcagcg ccaagttcat cgagaaaggc ggcgcggacc tcatcatcat ctacaacagc
      361 gggcgcttcc gcatgatggg ccacggcagc acctgcggcc tgatggcgta tggagatgcg
      421 aacgccatcg ccatggagat cggcgaatac gaggtgctgc ccgtggtgaa ggagatcccc
      481 gtgatctgcg gcgtgcatgc cacggacccg cgccgccgca tgtggcactg gctgggccgg
      541 gtgaaggaga tgggcttcag cggcgtgaac aacttcccca cgcacacgat cgtggacggg
      601 cacttccgcg gcgtgttgga ggagacgggc atgagcgtgc ggaaggagtt tgaaatggtg
      661 ggcctggcct cgcgcatgga ccttttcagc atcgtctatg tggccacgcc ggaggaggct
      721 gtggaaatgg cgaaaaacgg cgcggatgcc gtcatcgccc acgtcggcac caccgtcggc
      781 ggcagcatcg gcgtggtgaa tgccgtggtg gactgggact tcacggtgaa ccgcacgcag
      841 gagatcatag acgcggccaa aagcgtgaag ccggacattt tcactctcac ccacggcgga
      901 cccatcaaca cgccgaaaga tgtggaatac atcctcggca aaaccacggc ggacggcttt
      961 gtcggcgcca gcagcctgga acgcatgggc gtggaggact ccctgacgag cctgacgcgt
     1021 gagttcaagg gcgtgcggag gtgagggaga actcacaaaa tcaacatgca gtctccgtag
     1081 ctgaagaagc ggtatttttc ccgcacggct tcggcgtagg cgctcaaaat gaggtctttg
     1141 ccggcgaagg cgctgacgag catgagcagg gtggactttg gcaggtggaa gtttgtcagc
     1201 agcgcgccgc aggcgcggaa cttgaatcct ggataaatga aaatgtccgt gctgccacca
     1261 gctgcggtga tgcgtccgtg gtcgcgggtt acggattcga gagtgcgcat ggaggtggtg
     1321 ccgacggcga tgacgcggcg cgcggcggcg atggcggcgg cggtttcctc aggcacctgg
     1381 tagcgctcgg agtgcatgac atggtcctcg acacgctccg ctttcacggg ctgaaaggtg
     1441 cccgcgccga catgcagggt gacaaaggca tgcggcagcg cggccagcag atccggtgtg
     1501 aagtgaagcc cggctgtggg cgcggcgatg gcgcccgaag cgcgcgcata gacggtctgg
     1561 taacgctcgc gatcctccgc ccggtccatc cggcccatgt aatgcggcag ggccaggtgg
     1621 ccgtgcgcgt cctcatccac gggctggtcg aattcgatga cgcggtcgcc attttccagg
     1681 acttcgacca cggtgccggt ggcggcgccg atcccgacgg tgtggcccgg cctgaggcgg
     1741 cgaccgggct ttaccatgca gcgccagcgt gtggcggtga ggctgtccac gcgcagcagc
     1801 tctttttttc cgtcattgga gaagaagcgc gcggggacga cacgggtgtc attgagcacg
     1861 agaaggtcgc cttcccgcat gaagccaggc agctcgctga aatgacggtg ctccacgacc
     1921 ccgctgtcgc gctgcacgac catcatgcgc gaatccgccc tgccggccag aggctcggag
     1981 gcgatgagtt cgggcggtag ggtgtagtcg aagtcggcgg tgagcacgcg gtctggggga
     2041 atggcaggcg cggctttgac ggccgcgttc gaaggagaaa caaaaagcgg aatcctgccg
     2101 gtagca
//
LOCUS       DAOMYJ010000457         2083 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Prosthecobacter sp. isolate AMR_MDS_5629
            NODE_20720_length_2083_cov_20.926529, whole genome shotgun
            sequence.
ACCESSION   DAOMYJ010000457 DAOMYJ010000000
VERSION     DAOMYJ010000457.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867850
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prosthecobacter sp. (wastewater metagenome)
  ORGANISM  Prosthecobacter sp.
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Verrucomicrobiaceae; Prosthecobacter.
REFERENCE   1  (bases 1 to 2083)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2083)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.30
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:34:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,742
            CDSs (total)                      :: 4,684
            Genes (coding)                    :: 4,676
            CDSs (with protein)               :: 4,676
            Genes (RNA)                       :: 58
            tRNAs                             :: 55
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2083
                     /organism="Prosthecobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20720_length_2083_cov_20.926529"
                     /isolate="AMR_MDS_5629"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1965333"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(138..1019)
                     /gene="pssA"
                     /locus_tag="PLP58_23120"
     CDS             complement(138..1019)
                     /gene="pssA"
                     /locus_tag="PLP58_23120"
                     /EC_number="2.7.8.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009962661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003882 - CDP-diacylglycerol-serine
                     O-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--serine
                     O-phosphatidyltransferase"
                     /protein_id="HRJ11489.1"
                     /translation="MIEREPRIFVLPTLMTAGNILSGFVAILQIFKGREGLVTEHYYW
                     AIIAILAACLFDVLDGRVARLGGQESPFGRELDSIADIVSFGVAPALLVHDIVLSSIE
                     KPAGLGWLIASVYLVCGAMRLARFNCIAASDDKTNSKHFRGCPIPAAAGVIASLTLLM
                     LWLDEGNKEIGPWKYGLAVLMVLLSFLMMSDLEYPSFKSINWRTRRSPAWVLVSIIVV
                     AFTVRNWEWMPSVLFVSYLLYGLFRPWISRRWRRQIEIESETGEPADEPANGALNSDE
                     DSAAHESPAAPGKGSIA"
     gene            1105..1662
                     /locus_tag="PLP58_23125"
     CDS             1105..1662
                     /locus_tag="PLP58_23125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009960482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HRJ11490.1"
                     /translation="MTAPIPLTPETTLAQVLQAYPGARRSMFAKYHIGGCSSCAFSPQ
                     ETLAQLCARNDQIPVHELISHIQESHESDVRLQIPPAELAALLAGENPPRLLDARTRE
                     EHEAVHIPGSQLMTQEVVQEAFSQWDKAASVIIYDHTGTRSLDVAAYFIGHGFDDVRC
                     LSGGIDAYSAEADPSLPRYRVELEE"
     gene            1818..>2083
                     /locus_tag="PLP58_23130"
     CDS             1818..>2083
                     /locus_tag="PLP58_23130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ11491.1"
                     /translation="MSSLPDKVFERLDMLAEGSGLFRVVKEQRSKRENAILAKHPWKA
                     RLESLLHQASEHKADWVYFRLECEAGAPKPEAFIFDHTSSLSTGG"
BASE COUNT          389 a          717 c          616 g          361 t
ORIGIN      
        1 ttgaaccacc gggcgtggaa gcgcgccagg tcctcccggg tgagcgcggc cagctcctcc
       61 ccgcgtgtgg aggggtccgg cgcggccagg tgatgcagcg tggggaggcg ggtgtgctgg
      121 taaaagtccg ccatccttta tgcgatgctc cccttgccgg gcgcggcggg gctttcatgg
      181 gcggcgctgt cctcgtcaga gttgagggcg ccgttggcgg gttcgtcggc gggttcgccg
      241 gtctcgcttt cgatctcgat ctgcctgcgc cagcggcggg agatccacgg gcggaagagg
      301 ccgtagagca ggtagctcac aaagagcacc gagggcatcc attcccagtt ccgcacggtg
      361 aaggccacca cgatgatgga gaccagcacc caggcgggcg agcggcgcgt gcgccagttg
      421 atggacttga agctgggata ctccaggtcg ctcatcatga ggaaggagag cagcaccatg
      481 agcaccgcca ggccgtactt ccacgggccg atctccttgt tcccctcgtc cagccacagc
      541 atcagcagcg tcagggaggc gatgacgccc gccgccgccg ggatggggca gccgcggaag
      601 tgcttgctgt tcgtcttgtc atccgaggcg gcgatgcagt tgaagcgcgc cagccgcatg
      661 gccccgcaca ccaggtacac cgaggcgatc agccagccca ggccggccgg tttttcaatg
      721 ctggagagca cgatgtcatg caccagcagc gccggcgcca cgccaaagct cacaatgtcc
      781 gcgatcgagt ccagctcccg cccgaagggc gactcctgcc cccccagccg cgccacgcgc
      841 ccgtccagca catcgaaaag gcacgcggcc aggatggcga tgatggccca gtagtagtgc
      901 tccgtgacca gcccctcgcg ccccttgaag atttgcagga tggccacgaa tccggacagg
      961 atgttgcccg ccgtcatcag cgtgggcagc acaaaaattc ggggttcgcg ttcgatcatg
     1021 agcgcgcact gtaacccgcg gcccgcgctt gtccgcatga actttgcagt ttcctcgccg
     1081 ccgccgcgcg ttcatccttc cgccatgacc gcccccatcc cgctcacacc cgaaaccacg
     1141 ctcgcccagg tgctccaggc ctaccccggc gcgcgccgct ccatgttcgc caaataccac
     1201 atcggcggct gcagctcctg cgccttcagc ccgcaggaga cgctggccca gctctgcgcg
     1261 cgcaacgacc agatccccgt ccacgaactc atctcccaca tccaggaaag ccacgagtcc
     1321 gacgtgcggc tgcaaatccc ccccgccgaa ctcgccgccc tgctcgccgg ggaaaatccc
     1381 ccccgcctgc tcgacgcgcg cacccgcgag gagcacgagg ccgtccacat ccccggctcc
     1441 cagctcatga cccaggaggt ggtgcaggaa gccttttccc agtgggacaa agccgcctca
     1501 gtcatcatct atgaccacac cggcacccgc tccctcgacg tggccgccta cttcatcggc
     1561 cacggctttg acgacgtgcg ctgcctctcc ggcggcatag acgcctacag cgccgaggcc
     1621 gacccctccc tgccgcgcta ccgcgtggaa ttggaggaat gagcggggag cgagatcggc
     1681 agaggactgc cacacccgct cgacctccat gctctctttt gtgaattttg gcttgtcatc
     1741 ccatgttaaa aatgatgtgt tgttgaacag ttttagctga ctgccagcaa ttcttcatag
     1801 cgctcgtttt tccacccgtg tcatccctcc ccgataaagt cttcgaaaga ctcgacatgc
     1861 ttgccgaagg cagcgggtta tttcgtgtcg ttaaagaaca gagatcaaaa agggaaaatg
     1921 ccatcctggc aaagcatccc tggaaagccc gcctggaatc gctgcttcat caggccagtg
     1981 aacacaaggc agattgggtg tacttccgcc tcgaatgcga ggcgggtgcc cccaagccag
     2041 aagcgttcat ctttgaccac acttccagtc tgagtacggg tgg
//
LOCUS       DAOMYJ010000458         2071 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Prosthecobacter sp. isolate AMR_MDS_5629
            NODE_20900_length_2071_cov_22.608631, whole genome shotgun
            sequence.
ACCESSION   DAOMYJ010000458 DAOMYJ010000000
VERSION     DAOMYJ010000458.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867850
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prosthecobacter sp. (wastewater metagenome)
  ORGANISM  Prosthecobacter sp.
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Verrucomicrobiaceae; Prosthecobacter.
REFERENCE   1  (bases 1 to 2071)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2071)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.30
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:34:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,742
            CDSs (total)                      :: 4,684
            Genes (coding)                    :: 4,676
            CDSs (with protein)               :: 4,676
            Genes (RNA)                       :: 58
            tRNAs                             :: 55
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2071
                     /organism="Prosthecobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20900_length_2071_cov_22.608631"
                     /isolate="AMR_MDS_5629"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1965333"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>2071
                     /locus_tag="PLP58_23135"
     CDS             <1..>2071
                     /locus_tag="PLP58_23135"
                     /inference="COORDINATES: protein motif:HMM:TIGR02604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ11492.1"
                     /translation="GSTLPESGMRSVVVEASTFEFQIPEFTLSEGFTLAVVAAAPLVT
                     HPIMGCVDDRGRLFIGDAAGVNWNKAQLEANPPNRVLMLEDTDGDGVYEKSTVFADKM
                     TFPQGAYWHEGSLYVASPPGIWRLTDKDGDGIAEDRRQIVTGFDYTGNAADVHGPKVH
                     PNGRLYWCHGRKGHVISQKDGTLVHEGKASGIWSCLPDGGDVQWHSLGCADNPTGLAF
                     TPEGDIIGTCNLYYSNPRGDTLMHWLYGGVYERADQMQVIADLPRTLEKMPVIHNFGH
                     VAVSGCEFWRSQPGSPLYVTHFNTQRLVRMDLAKAGASYTATESEFLKIHNPDVHLTD
                     VIESTDGSLLVLDTGGWFRIGCPSGLMAKPDVLGAVYRITAGKKAAPVAAALPQPPAN
                     AKAAAALLKSPDAHARRRALEWTAQRLPHILEADAKAHADAAKLGTAIRLMMREPLDP
                     PLEHSLILAAQSMSLLAVSMDDLRKEKNPAALRRMLSSFIPDDSAAMNQHMGIAVQQL
                     DSADAALARVALGIVTQHPDADEWVAPTLIQWLSHAPTAARLTALEGFALVLRDQPQT
                     QKLLTAMLAAPQGETRRAALRVLAAQPVNAVHPGWLPPMQALLEKPADTGLPLTLDAI
                     KRLKTHPFDPQLQALADDSAQPLSLRLKALDAMKSLQLAGPTFDLIRATVTDPAASAA
                     ARIQAAAM"
BASE COUNT          374 a          906 c          508 g          283 t
ORIGIN      
        1 ggcagcaccc tccccgagtc cggcatgcgc tccgtggtgg tcgaagcctc gactttcgaa
       61 ttccaaatcc ccgagttcac cctctccgaa ggcttcactc tcgccgtcgt cgccgccgcg
      121 cccctcgtca cccaccccat tatgggctgc gtggacgacc gcggccgcct cttcatcggc
      181 gacgccgccg gcgtgaactg gaacaaagcc cagctcgaag ccaacccgcc caaccgcgtc
      241 ctcatgcttg aggacaccga cggcgacggc gtctatgaaa agagcaccgt cttcgccgac
      301 aagatgacat tcccccaggg cgcctactgg catgaaggca gcctctacgt cgccagcccg
      361 cccggcatct ggcggctcac cgacaaggac ggcgacggca tcgccgaaga ccgccggcag
      421 atcgtcaccg gcttcgacta caccggcaac gccgccgacg tccacggccc gaaagtccac
      481 cccaacggcc gactctactg gtgccacggg cgcaaaggcc acgtcatcag ccagaaggac
      541 ggcaccctcg tccacgaggg caaagccagc ggcatctggt cctgcctgcc cgacggtggc
      601 gatgtgcaat ggcactccct cggctgcgcc gacaacccca ccggcctcgc cttcaccccc
      661 gagggcgaca tcatcggcac ctgcaatctc tattacagca acccgcgcgg cgacaccctc
      721 atgcactggc tctacggcgg cgtctatgag cgcgccgacc agatgcaggt catcgccgac
      781 ctgccccgca ccctggagaa aatgcccgtc atccacaact tcggccacgt cgccgtcagc
      841 ggctgcgaat tctggcgctc ccagcccggc tctcccctct acgtcaccca cttcaacacc
      901 cagcgcctcg tgcgcatgga cctcgccaaa gccggtgcca gctacaccgc caccgagagc
      961 gagttcctca aaatccacaa ccccgacgtc cacctcaccg acgtcatcga gagcaccgac
     1021 ggctccctcc tcgtactcga cactggcggc tggttccgca tcggctgccc cagcggcctc
     1081 atggccaagc ccgacgtcct cggcgccgtc tatcgcatca ccgccgggaa aaaagccgcc
     1141 cccgtcgccg ccgccctgcc tcagccgccc gccaacgcca aagccgccgc cgcattatta
     1201 aaatcccccg acgcccacgc ccgccgccgc gccctcgaat ggaccgccca gcgcctcccc
     1261 cacatcctcg aagccgacgc caaagcccac gccgacgccg ccaagctcgg caccgccatc
     1321 cgcctcatga tgcgcgagcc gctcgacccg ccccttgagc actccctcat cctcgccgcg
     1381 cagagcatga gcctgctcgc cgtcagcatg gacgacctgc gcaaggagaa aaaccccgcc
     1441 gccctccgcc gcatgctctc cagcttcatc cccgacgact ccgccgccat gaaccagcac
     1501 atgggcatcg ccgtccagca gctcgacagc gccgatgccg ccctcgcccg tgtcgccctc
     1561 ggcatcgtca cccagcatcc cgacgccgac gaatgggtcg cccccacctt gatccaatgg
     1621 ctctcccatg cgcccaccgc cgcccgtttg accgccctcg aaggtttcgc cctcgtgctg
     1681 cgcgaccagc cgcagaccca aaaactcctc accgccatgc tcgccgcccc gcagggcgag
     1741 acacgccgcg ccgcattgcg cgtgctagcc gcccagcccg tcaatgccgt gcatccaggc
     1801 tggctccccc ccatgcaggc cctcctcgaa aaacccgccg acaccggcct ccccctcacc
     1861 ctcgacgcca tcaagcgcct caagacccac ccctttgatc cccaactcca ggccctcgcc
     1921 gacgactccg cgcagcccct ttcactccgg ctcaaagcgc tcgacgccat gaaaagcctc
     1981 cagctcgccg gccccacctt tgacctcatc cgagccaccg tcaccgaccc cgccgcctcc
     2041 gccgccgcgc gcatccaggc cgccgccatg c
//
LOCUS       DAOMYJ010000459         2058 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Prosthecobacter sp. isolate AMR_MDS_5629
            NODE_21084_length_2058_cov_14.566151, whole genome shotgun
            sequence.
ACCESSION   DAOMYJ010000459 DAOMYJ010000000
VERSION     DAOMYJ010000459.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867850
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prosthecobacter sp. (wastewater metagenome)
  ORGANISM  Prosthecobacter sp.
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Verrucomicrobiaceae; Prosthecobacter.
REFERENCE   1  (bases 1 to 2058)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2058)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.30
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:34:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,742
            CDSs (total)                      :: 4,684
            Genes (coding)                    :: 4,676
            CDSs (with protein)               :: 4,676
            Genes (RNA)                       :: 58
            tRNAs                             :: 55
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2058
                     /organism="Prosthecobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21084_length_2058_cov_14.566151"
                     /isolate="AMR_MDS_5629"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1965333"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..406
                     /locus_tag="PLP58_23140"
     CDS             <1..406
                     /locus_tag="PLP58_23140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ11493.1"
                     /translation="WGALGLLMRGESAVREHAAALQAALSDDSVLVRVTAAQSLGQHG
                     DPSRLSAALAALGALAAPQDHDVLTAMSALAAIEALGEKARPLHDEIAQMSPDGPSPD
                     GRYNSYVPRLIANIVPDAVAKAPKGGKGKKKK"
     gene            complement(429..1286)
                     /locus_tag="PLP58_23145"
     CDS             complement(429..1286)
                     /locus_tag="PLP58_23145"
                     /inference="COORDINATES: protein motif:HMM:NF013429.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HRJ11494.1"
                     /translation="MKKLLPLLALTALLSHAAEIPDTAKVGHFYAGCQTYTFRLFSVM
                     EAIEKTARAGGKTIEFYPRQKLSPEQPDAVFNHESAPEVIAAVKAKLAEKGVTAVAYG
                     VVRLGSNEVENRKVFEFCKTMGIGIVVTEPEVAALDQIEALVKEFDIKMAIHNHPRRP
                     LDRAYLFWDPEYVLSLVKDRDPRMGACADTGHWVRSGLDPVECVRILKGRIFDSHLKD
                     LDEKGNPKAKDVPYGGGVSNISAILAEYHKQGYPGPIHVEYESNWEDNVPDVTRCLDF
                     VRAWKPAVE"
     gene            complement(1331..>2058)
                     /locus_tag="PLP58_23150"
     CDS             complement(1331..>2058)
                     /locus_tag="PLP58_23150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ11495.1"
                     /translation="AARLLREEIQKAQGRPGQRADFLARFARILQHHDLARSGPAPVT
                     EPLEIKPKPRQQRTVPIGTAAAASKPLPGAPATALTSGFAPETQKPSIGFAELLFRDT
                     PVMETSSGPDWAKTAADAPPTIHPDEVLLPDSDYVPFWLRAAVFVGGSMILGAVFAHF
                     SGHAQWQKAKAPASSPPAAAAAATPVSVPAARQVITTAPEPPAAPPVDIPALSNAPDP
                     APSSPSLLMRPPGLKEDITGVPK"
BASE COUNT          326 a          620 c          760 g          352 t
ORIGIN      
        1 ctggggcgcg ctcggcctgc tcatgcgcgg cgagtccgcc gtccgcgagc acgccgccgc
       61 cttgcaggcc gcgctgtcag acgactccgt gcttgtgcgc gtcaccgccg cgcaaagtct
      121 ggggcagcac ggcgatccct cccgcctctc cgccgccctg gctgccctcg gcgccctcgc
      181 cgctccgcag gaccacgatg tgctgaccgc catgtcggcc ctcgccgcca ttgaggccct
      241 cggagagaaa gcgcggcctc tgcacgatga gatcgctcaa atgagccccg acggcccctc
      301 ccccgacggg cgctacaaca gctacgtccc gcggcttatc gccaacatcg ttccagacgc
      361 ggtcgccaaa gctcccaagg gcgggaaggg aaaaaagaaa aagtgagccg ggccgcgtcg
      421 cccccgcctc actcgacggc gggtttccag gcgcgcacaa agtcaaggca tcgcgtcaca
      481 tcggggacgt tgtcctccca gttggactcg tattcgacat ggatggggcc ggggtaaccc
      541 tgcttgtggt attcagccag gatggcgctg atgttggaga cgccgccgcc atagggcacg
      601 tctttggcct tgggattgcc tttttcatcc aggtccttga ggtggctgtc aaagatgcgg
      661 cccttgagga tgcgcacgca ctcgacgggg tcgagaccgg agcgcaccca atggccggtg
      721 tcggcgcagg cgcccatgcg cgggtcgcgg tctttgacga ggctgagcac atactctgga
      781 tcccagaaaa gataggcgcg gtcgagcggg cggcggggat ggttgtggat ggccatcttg
      841 atgtcgaact ccttcaccag ggcctcgatc tggtccagcg ccgcgacctc cggctcggtg
      901 acgacgatgc caatgcccat ggttttgcag aactcgaaga ccttgcggtt ttccacctcg
      961 ttcgagccga ggcggacgac tccgtaggcg acggcggtga cgcctttttc cgccagcttc
     1021 gctttcacgg cagcgatgac ttcgggcgcg gactcgtggt tgaagaccgc gtccggctgt
     1081 tcgggggaaa gtttctggcg cggataaaat tcgatggttt tgccgcctgc ccgggcggtt
     1141 ttttcaatgg cctccatcac ggaaaagagg cggaaagtgt aggtctggca gccggcgtaa
     1201 aagtgcccga ctttggccgt gtccgggatt tcggcggcgt gggaaagcag ggcggtcagc
     1261 gcgaggagcg ggaggagctt tttcatggca ggcggcaggg aacgggccgc gcgctgcgat
     1321 cttgcagagg ctacttcggc acgccggtga tgtcctcctt gagtcctggc gggcgcatga
     1381 gcagggatgg gctggacggg gcgggatcgg gcgcgtttga cagggcggga atgtccacgg
     1441 gcggggcggc cggaggctcc ggcgcggtgg tgatgacctg acgcgcggca ggcacggaga
     1501 caggcgtggc ggcagccgcg gcggcgggcg gagaggatgc gggtgccttg gctttctgcc
     1561 attgcgcgtg gccggagaaa tgcgcgaaaa cggcccccag gatcatggag ccgccgacaa
     1621 agacggcggc gcgcagccag aagggcacat agtcgctgtc aggcagcagg acttcatcgg
     1681 ggtggatggt cggcggcgcg tcggcggctg tcttggccca gtcgggaccg gaggatgtct
     1741 ccatgacggg cgtgtcgcga aaaagcagct cggcgaaccc gatcgagggt ttctgcgttt
     1801 cgggcgcgaa accgctcgtg agcgcggtgg cgggcgctcc aggcaggggc ttggatgcgg
     1861 ctgcggcggt gccgatgggc acggtgcgct gctggcgcgg cttgggtttg atctccagcg
     1921 gctcggtgac aggggccggg ccggaccggg ccaggtcgtg atgctggagg atgcgggcga
     1981 aacgggcgag aaaatccgcg cgctggccgg gacggccctg cgctttctgg atttcctccc
     2041 gcaggaggcg ggcggcgc
//
LOCUS       DAOMYJ010000460         2049 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Prosthecobacter sp. isolate AMR_MDS_5629
            NODE_21224_length_2049_cov_20.071214, whole genome shotgun
            sequence.
ACCESSION   DAOMYJ010000460 DAOMYJ010000000
VERSION     DAOMYJ010000460.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867850
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prosthecobacter sp. (wastewater metagenome)
  ORGANISM  Prosthecobacter sp.
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Verrucomicrobiaceae; Prosthecobacter.
REFERENCE   1  (bases 1 to 2049)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2049)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.30
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:34:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,742
            CDSs (total)                      :: 4,684
            Genes (coding)                    :: 4,676
            CDSs (with protein)               :: 4,676
            Genes (RNA)                       :: 58
            tRNAs                             :: 55
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2049
                     /organism="Prosthecobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21224_length_2049_cov_20.071214"
                     /isolate="AMR_MDS_5629"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1965333"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1691
                     /locus_tag="PLP58_23155"
     CDS             <1..>1691
                     /locus_tag="PLP58_23155"
                     /inference="COORDINATES: protein motif:HMM:NF037660.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HRJ11496.1"
                     /translation="PAHPATPAMPDPVIFLSAASVDLKPWREHLHTAFERAGFRVLTQ
                     DRSLRSAPGDVKRLLSETIEEATCVIHLAGLGYGSHATAPFPGHPAFQCSWTQFEYYH
                     AHQQGKDVIAFVCAPDLSTPGFTEEHASPAELALKQRLQREHRQRVATGEFTGTPLEG
                     KTPRTCNEAAHTPGKLLESVAAAVGTLRSLDLQDRERAQRELRSLAGSLAGVHRKLTA
                     ALVLLALVGLGVFWIKRDTAPLPAAVAQVGEGQEQLAAQMSQVQEALARIQQQTDPAK
                     DPVSQWPQERLETALAEQLQMKVDDLRALLVAGRTSLDALVAGQALLATGQSEDAGKK
                     FDLVLQQEQAAADRMRQAFAGKAQIAFDAVRYTEALDYYQKAAALVDKTADPLAWAEA
                     QHGVAFVLDKLARYKEAEPLMREVVRIWEEKLGPDDPKLATALNNLALLLQATNRLSE
                     AEPLMERVVKILENPGGEPLPNYAAALNNLAQLLKATNRLSEAEPLMRRALRIDEASL
                     GPDHPDVARDLNNLALLLQATNRLSEAELLMRRALRIDEASLGPDHPDVARDLNN"
     assembly_gap    1692..1791
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1792..>2049
                     /locus_tag="PLP58_23160"
     CDS             <1792..>2049
                     /locus_tag="PLP58_23160"
                     /inference="COORDINATES: protein motif:HMM:NF024816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HRJ11497.1"
                     /translation="AQLLQDTNRLSEAEPLLERVVKILENPGGEPLPNYAAALNNLAQ
                     LLKATNRLSEAEPLMRRALRIDEASLGPDHPDVARDLNNLA"
BASE COUNT          343 a          792 c          569 g          245 t
ORIGIN      
        1 acccgcccac cccgccactc ccgccatgcc agaccccgtc atcttcctca gcgcggcctc
       61 ggtggacctc aaaccgtggc gcgagcacct ccacaccgcc ttcgagcgcg ccggcttccg
      121 cgtcctcacc caggaccgca gcctgcgctc cgcgccgggg gatgtgaagc gcctgctctc
      181 cgagaccatc gaggaggcga cctgcgtcat ccacctcgcc ggcctcggct acggcagcca
      241 cgccaccgcc cccttccccg gccaccccgc cttccagtgc tcctggaccc agttcgagta
      301 ctaccacgcc caccagcagg ggaaggacgt catcgccttc gtctgcgccc cggacctctc
      361 cacccccggg ttcaccgagg agcacgccag ccccgccgag ctcgccctca agcagcgcct
      421 ccagcgcgag caccgccagc gcgtcgccac cggcgaattc accggcaccc ccctggaggg
      481 aaaaaccccc cgcacctgca acgaagccgc ccacaccccc ggcaagctcc tcgaaagcgt
      541 cgccgccgcc gtcggcaccc tcagaagcct tgacctccag gaccgggagc gggcgcagcg
      601 ggagctgcgg tccctcgccg gcagcctcgc cggcgtccac cggaagctca ccgccgccct
      661 cgtgctgctc gcgctcgtcg ggcttggcgt gttttggatc aaaagggaca ccgccccgct
      721 gcccgccgcc gtggcgcagg tcggcgaagg ccaggagcag ctcgccgcgc agatgtccca
      781 ggtgcaggag gccctggccc gcatccagca gcagaccgac cccgccaaag accccgtcag
      841 ccagtggccg caggagcggc tggagaccgc cctggcggag cagttgcaga tgaaggtgga
      901 tgacctccgc gccctcctcg tcgcaggccg caccagcctg gatgccctcg tcgccggcca
      961 ggccctgctc gccaccggcc agagcgagga cgccgggaag aaatttgacc tcgtcctcca
     1021 gcaggagcag gccgccgccg accgcatgcg ccaggccttc gcgggcaagg cccagatcgc
     1081 cttcgacgcc gtgcgctaca cggaggcgct cgactactac caaaaagccg ccgccctcgt
     1141 ggacaagacc gcggatcccc tcgcctgggc ggaagcgcag catggggtgg ccttcgtcct
     1201 cgacaagctc gcccgctaca aggaagccga gccactgatg cgcgaggtcg tccgcatctg
     1261 ggaggaaaag ctcggccctg atgaccccaa actcgccacc gcgctcaaca acctcgccct
     1321 gttgctccaa gccaccaacc gcctcagcga ggccgagccg ctgatggagc gggtggtgaa
     1381 gattctcgag aaccctggcg gcgaacctct cccgaactac gccgccgcgc tcaacaacct
     1441 cgcccagttg ctcaaggcca ccaaccgtct cagcgaggcc gagccgctga tgcgtcgcgc
     1501 gctgcggatt gacgaggcca gcctcggccc ggatcacccc gatgtcgcca gagacctcaa
     1561 caacctcgcc ctgttgctcc aggccaccaa ccgcctcagc gaggccgagc tgctgatgcg
     1621 gcgcgcgctg cggatagacg aggccagcct cggcccggat caccccgatg tcgccagaga
     1681 cctcaacaac cnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1741 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn ntcgcccagt
     1801 tgctccagga caccaaccgc ctcagcgagg ccgagccgct gttggagcgg gtggtgaaga
     1861 ttctcgagaa ccctggcggc gaacctctcc cgaactacgc cgccgcgctc aacaacctcg
     1921 cccagttgct caaggccacc aaccgtctca gcgaggccga gccgctgatg cgtcgcgcgc
     1981 tgcggattga cgaggccagc ctcggcccgg atcaccccga tgtcgccaga gacctcaaca
     2041 acctcgccc
//
LOCUS       DAOMYJ010000461         2022 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Prosthecobacter sp. isolate AMR_MDS_5629
            NODE_21683_length_2022_cov_19.399593, whole genome shotgun
            sequence.
ACCESSION   DAOMYJ010000461 DAOMYJ010000000
VERSION     DAOMYJ010000461.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867850
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prosthecobacter sp. (wastewater metagenome)
  ORGANISM  Prosthecobacter sp.
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Verrucomicrobiaceae; Prosthecobacter.
REFERENCE   1  (bases 1 to 2022)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2022)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.30
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:34:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,742
            CDSs (total)                      :: 4,684
            Genes (coding)                    :: 4,676
            CDSs (with protein)               :: 4,676
            Genes (RNA)                       :: 58
            tRNAs                             :: 55
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2022
                     /organism="Prosthecobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21683_length_2022_cov_19.399593"
                     /isolate="AMR_MDS_5629"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1965333"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..587
                     /locus_tag="PLP58_23165"
     CDS             <1..587
                     /locus_tag="PLP58_23165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ11498.1"
                     /translation="RGGWRYEPSSSDSDISVSVWQLMSLRAAKNCGITVPREAIDQAV
                     AYIKRSYRAERDSQGNLKQLEAAFSYEPYGGRQTFSTTAAGLLSLQVAGQYDAPEVLG
                     SSNWLLKFPPEVNEPWFFYGCYYYAQGMYQRGGDHAATARAKTEQMLTSTQDPDGAWF
                     PRNGNEKSAGPVYATALALLSLSVYHHFLPIYQK"
     gene            complement(596..1858)
                     /locus_tag="PLP58_23170"
     CDS             complement(596..1858)
                     /locus_tag="PLP58_23170"
                     /inference="COORDINATES: protein motif:HMM:NF012640.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate synthase component I family
                     protein"
                     /protein_id="HRJ11499.1"
                     /translation="MSTLLPISAKTPAGRPRGDGAETAQGLTPAQMARRMAGMEGLVW
                     LDSSRPGEGALSMLTALPCEVLAGRMDTDWQRVEEELEAGQKAGLAGGLFGWAGFDGR
                     FVLGVFPHALVYDHDRRCWFDSGGLAARIPAVAAAAGPVPRLDFSPQVRREDFLSQVR
                     RAQELIAAGDIYQVNLAHSWQAAWPRGADALSAYQRLRRVSPAPHAAFLDLAGVTVLS
                     ASPELFLKMSGREIMTRPIKGTRPRFPGDPARDAASLSALLASEKERAELLMITDLER
                     NDLGRVCEYGSVRVDGLWEVESFAQVFHLVSTVGGVMRPEVSHAAAFRACFPGGSITG
                     APKKRAMEIIQELEAAPRGLYTGAVGYFGFDGESRWSIAIRTAVREGERVTFHAGSGI
                     VADSVPELEWEETLHKAAGLLAAWGLPR"
BASE COUNT          355 a          765 c          604 g          298 t
ORIGIN      
        1 accgcggcgg ctggcgctac gaacccagca gcagcgacag cgacatctcc gtctccgtct
       61 ggcagctcat gtccctgcgc gccgccaaga actgcggcat caccgtgccc agagaggcca
      121 ttgaccaggc cgtggcctac atcaagcgca gctaccgcgc cgagcgcgac agccagggca
      181 acctcaagca gctcgaggcc gccttcagct acgagcccta cggcgggcgg cagaccttct
      241 ccaccaccgc cgccggcctg ctctcactcc aggtcgccgg gcagtacgac gcgccggagg
      301 tgctcggctc ctcgaactgg ctgctcaagt ttccccccga ggtcaacgag ccctggttct
      361 tttacggctg ctattactac gcccagggca tgtaccagcg cggcggcgac cacgccgcca
      421 ccgcccgcgc caaaaccgag cagatgctca cctccacgca ggatcccgac ggcgcttggt
      481 tcccccgcaa cggcaatgaa aaaagcgccg gccccgtcta tgccaccgcg ctcgccctgc
      541 tgagcctcag cgtctatcat catttcctgc cgatttatca gaagtgagcg ccgtgtcagc
      601 gcggcaagcc ccacgccgcc agcaggccgg cggccttgtg cagcgtctcc tcccattcca
      661 gttccggcac actgtcggcc acgatgcccg acccggcgtg gaaggtgacg cgctcccctt
      721 ccctcacagc ggtgcggatg gcaatgctcc agcggctttc gccatcgaac ccgaaatacc
      781 cgacggcccc cgtgtaaaga ccgcgcggcg ccgcctccag ctcctgaatg atctccatcg
      841 cgcgcttctt cggcgcgcca gtgatgctgc cgccggggaa gcaggcgcgg aaagcggcgg
      901 cgtggctgac ctccgggcgc attaccccgc cgactgttga caccaggtga aacacctgcg
      961 cgaagctctc cacctcccac agaccgtcca cgcgcacgct gccgtattcg cacacgcggc
     1021 ccaggtcgtt gcgctccagg tcggtgatca tcagcagctc ggcgcgctcc ttctcactgg
     1081 ccagcagcgc cgagagggaa gccgcatcgc gcgcgggatc gcctgggaaa cgcgggcgcg
     1141 tgcctttgat ggggcgggtc atgatctccc gcccgctcat tttcaaaaaa agctccggag
     1201 aggccgaaag caccgtcaca ccagccaaat ccagaaaagc cgcgtgcggc gcgggcgaga
     1261 cgcggcgcag ccgctgatag gcggacaagg cgtccgcacc gcgcggccag gcggcctgcc
     1321 aggaatgagc cagattcacc tgataaatgt cacccgccgc gatgagttcc tgcgcgcgcc
     1381 ggacctggct gagaaaatcc tcccgccgca cctgcgggct gaagtcaaga cgcggcaccg
     1441 gcccggcggc ggcggccaca gcgggaatcc gcgccgcgag accgcctgaa tcgaaccaac
     1501 agcgccggtc atggtcataa acgagcgcat gaggaaagac tcccaggaca aaccgcccgt
     1561 caaaaccggc ccagccgaac aaaccgcccg ccagcccggc cttttgcccg gcctccagct
     1621 cctcctccac gcgctgccag tccgtgtcca tgcggccggc cagcacctcg cacggcagcg
     1681 cggtgagcat ggagagcgcg ccctcgccgg gccgggagct gtccagccac accagccctt
     1741 ccattcccgc catgcggcgc gccatttgcg ccggagtcag gccctgcgcc gtctccgcgc
     1801 cgtctccgcg cggacgcccg gcgggagttt tggcggagat tggcaggagc gttgacactg
     1861 gagtgcgtgg accgtaccat acgaatttcc tgcggcaatc ggcatggctg aaaccacctc
     1921 cattctcaac gctgaccggg gccgcgcctt caccgctgcc ctcgtctttc tctgcgccct
     1981 ggcggcggcg cagattctgc tcgtggcgcg cgcgctcatc ct
//
LOCUS       DAOMYJ010000462         2019 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Prosthecobacter sp. isolate AMR_MDS_5629
            NODE_21722_length_2019_cov_18.276477, whole genome shotgun
            sequence.
ACCESSION   DAOMYJ010000462 DAOMYJ010000000
VERSION     DAOMYJ010000462.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867850
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prosthecobacter sp. (wastewater metagenome)
  ORGANISM  Prosthecobacter sp.
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Verrucomicrobiaceae; Prosthecobacter.
REFERENCE   1  (bases 1 to 2019)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2019)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.30
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:34:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,742
            CDSs (total)                      :: 4,684
            Genes (coding)                    :: 4,676
            CDSs (with protein)               :: 4,676
            Genes (RNA)                       :: 58
            tRNAs                             :: 55
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2019
                     /organism="Prosthecobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21722_length_2019_cov_18.276477"
                     /isolate="AMR_MDS_5629"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1965333"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1221)
                     /locus_tag="PLP58_23175"
     CDS             complement(<1..1221)
                     /locus_tag="PLP58_23175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ11500.1"
                     /translation="MKSSRFRIALLALLCGTSLLRADYLIMKNGDKVEGTILSETPDA
                     VRMKYRLTPKIWDEKDFPRAEIDQVVKQRPEEVEIVELRKILPTEDLLTADKYEQIIQ
                     DRLRPFVNQYKDTPEAKEAEGIIATLQDEKKKVSDGGIKLEGKWLSAAEARGEEYNIE
                     AYKLLAKMRAHMAAGDWLEALRVFENFSKKKPGYTGSTYYPAAITEALASMEKWEVLL
                     SKMGQEQPALDKTRKDGLKKLEEADQARTKAAIEEERAKWQAQAEADRRAQIGWIAPY
                     RYDLPSILNMQKKIVAERSRLTALNLEDMRIRNEAISQVYRKIGQEDYNGGVAAFERI
                     SSLSNVPEYRDVINDLKSKMLALYNTLVRRQMAAAQSAAAGSSAIGGTTTTGQDERVA
                     AILAEASGQAPAAAP"
     gene            complement(1362..1592)
                     /locus_tag="PLP58_23180"
     CDS             complement(1362..1592)
                     /locus_tag="PLP58_23180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ11501.1"
                     /translation="MPFDTALSRAVMRGQSTGLKAVINSVPVISGAVVNSVTPLPPGD
                     SRRTSVALICGVLSFSFGLSPSLLAEGSEAAV"
     gene            complement(1739..>2019)
                     /locus_tag="PLP58_23185"
     CDS             complement(1739..>2019)
                     /locus_tag="PLP58_23185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016290497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sigma factor-like helix-turn-helix DNA-binding
                     protein"
                     /protein_id="HRJ11502.1"
                     /translation="LVLSLYYFEELNLKEIGQVLEVTESRVSQLHTPAGLVQPRAAPP
                     RASRRPSGIIKLRRIIGCHANTGCRTGQKRMPTGAQDQRIQRGSRTRK"
BASE COUNT          327 a          589 c          647 g          456 t
ORIGIN      
        1 aggagcggcg gcgggggcct ggccgctggc ctcggccagg atggcggcca cgcgctcgtc
       61 ctggccggtg gtcgtggtgc cgccgatggc cgaggagcct gcggcggcgc tttgcgcggc
      121 ggccatctgg cggcgaacca gggtgttgta aagggcgagc attttgctct tcaggtcgtt
      181 gatcacgtcg cggtattcag ggacattcga cagggatgaa atgcgctcaa aggcggcgac
      241 gccgccgttg tagtcctcct gcccgatttt acggtaaacc tggctgatgg cctcgttgcg
      301 gatgcgcatg tcctccaggt tgagggccgt gagccgtgag cgctcggcga cgatcttctt
      361 ctgcatgttg aggatggagg gaagatcgta cctgtaaggg gcgatccaac caatctgtgc
      421 gcggcggtcg gcttcggcct gggcctgcca tttggcgcgc tcctcctcaa tggccgcttt
      481 ggtgcgggcc tggtcggcct cctccagttt tttcaagcca tctttgcgcg tcttgtccag
      541 cgctggctgc tcctggccca ttttggaaag gagaacctcc catttctcca tgcttgccag
      601 cgcttcggtg atggcggcgg ggtagtaggt ggagcctgtg tagccgggct tcttcttgga
      661 gaagttctca aaaactcgca gcgcttcgag ccagtcgccc gccgccatgt gcgcgcgcat
      721 tttcgccagc agtttgtagg cttcgatgtt gtactcctcc cccctggctt cggcggcgga
      781 gagccatttg ccctcgagtt tgatgccgcc gtcggagact tttttcttct cgtcctggag
      841 cgtggcgatg atgccctcgg cctccttggc ctcgggcgtg tccttgtatt ggttcacaaa
      901 gggacgcaac cggtcctgga tgatctgctc gtatttgtcg gcggtcagca gatcttccgt
      961 gggcaggatt ttccgcagtt cgacaatctc cacctcctcc ggccgctgct tcaccacctg
     1021 gtcaatctcg gcgcgcggga agtccttctc gtcccagatt ttcggcgtca gccggtattt
     1081 catgcgcacg gcgtcggggg tttcggagag tatggtgccc tccactttgt cgccgttttt
     1141 catgatcaaa taatcggcgc gcagcaggga ggtgccgcac agaagtgcca ggagggcgat
     1201 gcggaatcgg ctggatttca tggtctgtga tgtcgtggta tgaatggatg ttccggcgcg
     1261 gagacgcctt atttcacgga tttaatctgg attcgccatg cgagtaaagc ccgaaactgc
     1321 ctgtttttaa aacatgcgcc cgctgctgcc ggcggcatgc atcaaacggc ggcctcgctg
     1381 ccctccgcca gcaggctggg ggagaggccg aagctgaagg aaagcacgcc acaaatgagc
     1441 gccacggacg tcctgcgcga atctcccggc ggcagcggcg ttaccgaatt caccactgcg
     1501 ccgctgatca ccggcacgga attaatgacg gccttcaatc ccgtggactg gcctcgcatc
     1561 acggcgcggg agagggcggt gtcgaagggc atggccggag aggggcaagg gggcagggag
     1621 acacctttgc tggattgccg cgtttgaatg tcgggttgaa actggtcaac ggcttgctgc
     1681 gatatccgaa tgtcggggtt gaacttcggg tccgtgccca cccgcgatac cccgcccgct
     1741 attttcgggt acgcgacccg cgctggatgc gctgatcctg cgcgccggtt ggcattcgct
     1801 tttgaccggt ccggcatccc gtgtttgcat ggcagccgat gatccgccgc agctttatga
     1861 ttcccgacgg ccgccgagag gcgcgcggcg gtgccgcacg cggctgcacc aaccccgccg
     1921 gcgtgtgtaa ttgagacacg cggctttctg taacttctag aacttggcca atttctttga
     1981 ggttaagctc ctcaaaataa tacagagata aaacaagcc
//
LOCUS       DAOMYJ010000463         1984 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Prosthecobacter sp. isolate AMR_MDS_5629
            NODE_22291_length_1984_cov_22.209435, whole genome shotgun
            sequence.
ACCESSION   DAOMYJ010000463 DAOMYJ010000000
VERSION     DAOMYJ010000463.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867850
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prosthecobacter sp. (wastewater metagenome)
  ORGANISM  Prosthecobacter sp.
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Verrucomicrobiaceae; Prosthecobacter.
REFERENCE   1  (bases 1 to 1984)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1984)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.30
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:34:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,742
            CDSs (total)                      :: 4,684
            Genes (coding)                    :: 4,676
            CDSs (with protein)               :: 4,676
            Genes (RNA)                       :: 58
            tRNAs                             :: 55
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1984
                     /organism="Prosthecobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22291_length_1984_cov_22.209435"
                     /isolate="AMR_MDS_5629"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1965333"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(210..1706)
                     /locus_tag="PLP58_23190"
     CDS             complement(210..1706)
                     /locus_tag="PLP58_23190"
                     /inference="COORDINATES: protein motif:HMM:NF014500.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HRJ11503.1"
                     /translation="MSPVLAAASPDSWPFIVLAISVAFIIIGISKLRLHPFLALVLAA
                     MLAGLLTHKESWDVVQKDGKVKNLPAMVGVVEMVSKGLGDTARDIAISIALAAIIGMC
                     LMESGGADKVVRRFLAFFGEKRAGWALLWSTFILSAPIFFDTMFMLMAPLAMALRLRT
                     GKDYTLYILAVCCGGTITHSMTVPHPGPVAVVDDLRLNVGESIIGGMVIGAVTCFFGY
                     FVARWLNSKTDTPLRETNGLSLEELAGVSARQESQLPGFFWSITPVILPIVLISMTTV
                     FELASLSSKAGPGWGAGFIALCGGDAGFATVRGWVDFIGHKNIALLIGAVISLWVLAR
                     QRGFGLKKLEQLVGPPLETAGMIILITSAGGAFGLALRNAGVGDAVESLAQGYSLNLI
                     LLGWLVAAVVRVAQGSATVAMLTASSIMAPMIAGGALDCHPVYLFTAIGFGAMFFSWM
                     NDSGFWVVSRLSGMTEKETLRTWSIQLTANSVIGLLVTLALSKLLPLV"
BASE COUNT          355 a          642 c          701 g          286 t
ORIGIN      
        1 ggccgccatt tgcccgcggc gccggcctcg cgcgccagca gggcatgcag cgcgatgccg
       61 gagaggccgg tgaggaattc acggcgggga atgaaggcgg cggggggcat gtggaggccg
      121 gtcacgccac tggtacggcg cccgggcggc ggattcttca atgacaggcc cgcaaatgcg
      181 cgcctcatcc cgcccgccag cgcgccgcgt cagaccagcg gcagcagctt gctgagggcc
      241 agggtgacga gcaggccgat gacggagttg gcggtgagct ggatgctcca ggtgcgcagg
      301 gtctcttttt ccgtcatgcc gctgaggcgg gagacgaccc agaagccgct gtcgttcatc
      361 caggagaaga acatggcccc gaagccgatg gcggtgaaaa gatagacggg gtggcagtcg
      421 agcgcgccgc cggcgatcat cggcgccatg atggaggagg cggtgagcat ggcgacggtg
      481 gcggagccct gcgccacgcg gacgacggcg gcgacgagcc agcccaggag gatgagattg
      541 aggctgtaac cctgcgccag cgactcgacg gcgtcaccca cgccggcgtt gcgcagggcc
      601 aggccgaagg cgccgcccgc gctcgtgatg aggatgatca tgccggcggt ctccagcggc
      661 ggccccacga gctgctcgag ctttttcagg ccgaacccgc gctgccgcgc cagcacccac
      721 agcgaaatga cggccccgat gagcagggcg atgtttttgt gcccgatgaa gtccacccag
      781 ccgcgcaccg tggcgaagcc cgcgtcaccg ccgcacaggg cgatgaagcc cgcgccccag
      841 ccggggccgg ccttggatga gaggctggcc aattcgaaca cggtggtcat gctgatgagc
      901 acgatgggca ggatgacggg cgtgatgctc cagaagaatc cgggaagctg gctctcctgc
      961 cgcgcgctga cgccggccag ctcctccagg gagaggccgt tggtctcccg caggggcgtg
     1021 tcggtcttgc tgttgagcca cctggcgacg aagtagccga agaagcaggt gacggcgccg
     1081 atgaccatgc cgccgatgat ggactcgccc acattgagcc gcagatcatc caccacggcc
     1141 accgggccgg ggtgcggcac ggtcatcgag tgggtgatgg tgccgccgca gcagacggcc
     1201 aggatgtaaa gcgtgtagtc cttgccggtg cgcagccgca gggccatggc gagcggggcc
     1261 atgagcatga acatggtgtc gaaaaaaatg ggcgcgctga ggatgaaggt gctccacagc
     1321 agcgcccagc cggcccgctt ttcgccgaag aaggccagga agcggcgcac caccttgtcc
     1381 gcgccgccgc tctccatcag gcacatgccg atgatggccg cgagcgcgat ggagatggcg
     1441 atgtcgcgcg cggtgtcgcc caggcccttg gagaccatct ccaccacgcc gaccatggcg
     1501 ggcaggtttt tcaccttgcc gtccttctgc accacatccc agctctcctt gtgggtgagc
     1561 aggcccgcga gcatggcggc gagcaccagc gccagaaagg gatgcaggcg cagcttgctg
     1621 atgccgatga tgatgaaggc cacgctgatg gcgagcacga tgaacggcca ggagtcgggg
     1681 gaggcggcgg cgaggacggg agacataaat cagcggcgat gctggcgggt ttccgccggt
     1741 tcatccaccc aaaaatcaca cccgcccgcc tttcccgcgg attcccgcgg acggttcggc
     1801 gaatccccgc gccgcatcac caggagccgc gggccggtca cgggcaaagc atttgctgga
     1861 aatttgaaac ccctgcctta ttgcttcccc gcacgatcat gtccccctcc cgaattcctt
     1921 gttcccgcgc cgcctggacc ggcgtggcgg gctgccttct cctcagcggg gccggcgcgc
     1981 agac
//
LOCUS       DAOMYJ010000464         1951 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Prosthecobacter sp. isolate AMR_MDS_5629
            NODE_22871_length_1951_cov_22.487342, whole genome shotgun
            sequence.
ACCESSION   DAOMYJ010000464 DAOMYJ010000000
VERSION     DAOMYJ010000464.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867850
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prosthecobacter sp. (wastewater metagenome)
  ORGANISM  Prosthecobacter sp.
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Verrucomicrobiaceae; Prosthecobacter.
REFERENCE   1  (bases 1 to 1951)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1951)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.30
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:34:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,742
            CDSs (total)                      :: 4,684
            Genes (coding)                    :: 4,676
            CDSs (with protein)               :: 4,676
            Genes (RNA)                       :: 58
            tRNAs                             :: 55
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1951
                     /organism="Prosthecobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22871_length_1951_cov_22.487342"
                     /isolate="AMR_MDS_5629"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1965333"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            89..>1951
                     /locus_tag="PLP58_23195"
     CDS             89..>1951
                     /locus_tag="PLP58_23195"
                     /inference="COORDINATES: protein motif:HMM:NF019552.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heparinase II/III family protein"
                     /protein_id="HRJ11504.1"
                     /translation="MPAAADYLRGVQKTRPRLIHAAAGWEYLKGQASSNPVAGKMLEN
                     LKAGGEKVLEAPELTRIFGEMRSTVTPGSKALFRMATLGALNFVDGDPRWKERGVREL
                     IAITDPTVFTNWYVDEPEVLADFLIAASLGYDYFRDGLNDQQAAAVRTYMVEKGIGAL
                     AAHLEGEPMPESARGRAPGTSASKAPAKGAAKKADGPEQPDVEHMAGAAALILAAICL
                     ADEEPSAAKTAVDAAAKMFGAGMQMFAPAGIWPEGMEAGETVLDYAIMVMQTLKANSG
                     SDFGFSLLEGIPQAGLARVHLVGPTNQLFNYGDTNSTALSRAWVSTWLAGAHGNMGTK
                     ALTAGTAPGADTAHLNLAGHFMYFNPHAAGDGTPDSLDYTSPGALVAAHRTGWDKDAL
                     YIAVKGGDNSFVHNQLDLGAFVLDAGGTRWGIELGEESDRAPGFTSTDRNKRYELYVE
                     GTAGQNTLWMEGNQEFDAKAAVVMAHSTPALAMSVIDLGKAYSKPAKDALRGVMITRG
                     DKPQVLVQDEIGVKNNTHVVWTMHTRAEVSADGKKAVLTDKGRTLHATLLSPAQASFS
                     VGEPPEPTNEQMKKLTGIRVLKVDLGTTKGDHTIAVAFALDEAPAEAPVKPIAEW"
BASE COUNT          389 a          705 c          578 g          279 t
ORIGIN      
        1 cgccacgaag ggcgccctcc cggctccgac gggcgcaccg gcgcctccac gccccacatt
       61 gaccatcacc cccgtgaaag gtttcaaggt gcccgccgcc gcagactacc tgcgcggagt
      121 gcagaagacc cgcccgcgcc tcatccacgc cgccgccggc tgggagtatc tgaaaggcca
      181 ggcatcatca aaccccgtgg ccggcaaaat gctggagaac ctcaaggctg gcggtgaaaa
      241 ggtgctcgaa gcacccgagt tgacccgcat ttttggcgag atgcgcagca ccgtcacacc
      301 ggggtccaag gcgctgttcc gcatggccac gctgggcgcg ctgaatttcg tggacggcga
      361 cccgcgctgg aaggagcgcg gcgtgcgcga gctcatcgcc atcaccgatc ccaccgtctt
      421 caccaactgg tatgtggacg agcccgaggt gctcgcggac ttcctcattg ccgcctccct
      481 cggctatgat tatttccgcg acggtctcaa cgaccagcaa gccgcagccg tgcgcactta
      541 catggtggaa aagggcatcg gcgccctcgc cgcccatctc gaaggcgaac ccatgcctga
      601 atccgcgcgc gggcgcgcac ccggcacctc ggccagcaaa gcgcccgcca agggggccgc
      661 caaaaaagcc gacggccccg agcaacccga tgtcgagcac atggccggcg cggcggcgct
      721 catcctcgcc gccatctgcc tcgctgacga ggagccttct gcggcaaaga ccgcagtgga
      781 cgcggcggca aaaatgttcg gcgcgggcat gcagatgttc gcccccgccg gcatctggcc
      841 ggagggaatg gaggccgggg aaaccgttct cgactacgcc atcatggtca tgcagaccct
      901 caaggccaac agcggttccg acttcggttt cagccttttg gaaggcatcc cgcaggcggg
      961 gctggcgcgg gttcatctgg tggggccgac gaaccagctt tttaactacg gcgacaccaa
     1021 ctccaccgcc ctgagccgcg cctgggtgtc cacatggctg gccggcgcgc atggaaacat
     1081 gggcacgaag gccctcaccg ccggcaccgc ccccggagcc gacaccgccc atctgaatct
     1141 ggcaggccac ttcatgtact tcaatcccca cgccgccggc gatggcacgc cagacagtct
     1201 cgactacacc tcccccggcg cgctcgtggc cgcgcaccgc accggctggg acaaggacgc
     1261 cctttacatc gccgtcaaag gcggggacaa cagtttcgtt cacaaccagc tcgaccttgg
     1321 cgctttcgtc ctcgacgcgg gcggcacgcg ctggggcatc gaacttggcg aggagagcga
     1381 ccgcgcgccc ggcttcactt ccaccgaccg caacaaacgc tacgagcttt atgtcgaggg
     1441 caccgccggc cagaacacgc tctggatgga gggcaaccag gagtttgacg ccaaggccgc
     1501 cgtggtcatg gcccattcca ctccggcgct cgccatgagt gtcattgacc tcggcaaagc
     1561 ctactcgaag cccgccaagg acgccctccg cggcgtcatg atcactcgcg gtgacaagcc
     1621 gcaggtgctt gtccaggatg aaatcggcgt caaaaacaac acccatgtcg tctggaccat
     1681 gcacacccgc gccgaagtca gcgctgacgg caaaaaagcc gtgctcaccg acaaaggccg
     1741 aaccctccat gccacgctgc tctcccccgc gcaggccagc ttcagcgtcg gagagccgcc
     1801 cgaaccgacc aacgagcaga tgaaaaaact caccggcatc cgcgtgctca aagtggacct
     1861 cggcaccacc aagggggatc acaccatcgc ggtcgccttc gcgctggacg aagcccccgc
     1921 cgaagcaccc gtgaaaccca tcgcggaatg g
//
LOCUS       DAOMYJ010000465         1951 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Prosthecobacter sp. isolate AMR_MDS_5629
            NODE_22874_length_1951_cov_19.260549, whole genome shotgun
            sequence.
ACCESSION   DAOMYJ010000465 DAOMYJ010000000
VERSION     DAOMYJ010000465.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867850
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prosthecobacter sp. (wastewater metagenome)
  ORGANISM  Prosthecobacter sp.
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Verrucomicrobiaceae; Prosthecobacter.
REFERENCE   1  (bases 1 to 1951)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1951)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.30
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:34:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,742
            CDSs (total)                      :: 4,684
            Genes (coding)                    :: 4,676
            CDSs (with protein)               :: 4,676
            Genes (RNA)                       :: 58
            tRNAs                             :: 55
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1951
                     /organism="Prosthecobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22874_length_1951_cov_19.260549"
                     /isolate="AMR_MDS_5629"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1965333"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..563)
                     /locus_tag="PLP58_23200"
     CDS             complement(<1..563)
                     /locus_tag="PLP58_23200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ11505.1"
                     /translation="MRFSIILIALALLPVMLLAHTWYYARKQMPLLHEQVLSILDRHH
                     IQDVKVGLNHLDLSVSGKAAGTPDMDAALDELRALRPLRLMVQDITVPSALRADLAGD
                     RLVVNGSLPLDADLDSLRARLSDWRPDLHVETGGVRRHPQVRWPEREAGHWDADGPLL
                     APLMPLLAVKARLEVVLHSGGLRATGIL"
     gene            695..1615
                     /locus_tag="PLP58_23205"
     CDS             695..1615
                     /locus_tag="PLP58_23205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009964934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HRJ11506.1"
                     /translation="MSVFIAGTVALDNVKTPHDAQENLLGGSASYAALAAGIFCQPVH
                     LVGVIGHDFPEAHLRLLESRGVRLEGLERSSGESFTWSGEYHDDMNSRTTHKVAVNVL
                     EHWQPKLTAEAARAKIAVLANMSPDNQIQALEQAEGADFIAADTMDLWISIANERLHD
                     LLKRVDLLVINDGEAKEFAKTTNLVEAGRRLQAKGPRFVVVKRGEHGSFLFGERPEDF
                     FACSAYPLDSVFDPTGAGDSFLGGMAGWLAANGRTKPAFADLKNAVVHGSVTASFTCE
                     AFSTHKLQTVTAADVSRRLEELKKFTSFEA"
BASE COUNT          355 a          613 c          675 g          308 t
ORIGIN      
        1 aggatgccgg tggcgcgcag gccgccgctg tgaagcacga cttcgaggcg ggctttcacc
       61 gcgagcaagg gcatgagcgg agccagcagg gggccgtccg catcccagtg gccggcctcc
      121 cgctcaggcc agcgcacctg gggatggcgg cgcaccccgc ccgtctcgac atgcagatcg
      181 gggcgccagt cggagaggcg cgcgcgcagc gagtcgagat cggcgtcaag gggcagcgag
      241 ccatttacaa caagcctgtc acccgcgagg tcggcgcgca aagcagaggg gaccgtgatg
      301 tcctggacca tcagccgcag cgggcgcagc gcgcgcagct catcaagcgc ggcgtccatg
      361 tccggcgtgc cggcggcctt gccggaaacg gagagatcga ggtggttcag cccgactttg
      421 acatcctgga tgtgatgccg gtcgaggatg gagaggacct gctcatgcag gagcggcatc
      481 tgcttcctgg cataatacca tgtgtgcgcc agcagcatca ccggcagcag cgcgagggcg
      541 atgaggatga tggaaaaacg catggccgcc gtgggtgggg gatcggtgat tgacacccgg
      601 cacgcgcacg ggatactcgc gccgccttcg ccgggcggga gtgtcagcct gccgtggcgc
      661 gcgggcaaga acttcttccc tcatcatacc ctccatgtct gtcttcatcg ccggaaccgt
      721 cgccctcgac aatgtgaaaa ccccgcacga cgcgcaggaa aacctgcttg gcggctcggc
      781 gtcctacgcg gcgctggcgg cgggcatttt ctgccagccg gtgcacctgg tcggcgtcat
      841 cgggcacgac ttcccggagg cgcatctgcg cctgctggag agccggggag tcaggctgga
      901 gggcctggag cgcagcagcg gggaatcctt cacctggagc ggcgagtatc acgacgacat
      961 gaacagccgc acgactcaca aggtggcggt gaatgtgctc gagcactggc agcccaaatt
     1021 aacggcggag gcggcgcggg cgaaaatcgc ggtcctggca aacatgagtc ctgacaatca
     1081 aattcaggct ctggagcagg cggagggcgc ggacttcatc gcggcggaca cgatggacct
     1141 ctggatcagc atcgccaacg agcgcctgca cgacctgctc aagcgcgtgg accttctggt
     1201 catcaacgac ggggaggcga aggagtttgc gaagacgacg aacctggtcg aggcgggccg
     1261 caggctccag gccaaggggc cgcgcttcgt ggtggtgaag cgcggcgagc acggcagctt
     1321 tttgttcggc gaaaggccgg aggatttctt cgcctgctcg gcttatccgc tggactcggt
     1381 cttcgacccc accggcgccg gggactcctt cctcggcggc atggcgggct ggctggcggc
     1441 gaacggacgc accaagccgg cctttgccga cctgaaaaac gccgtggtcc acggcagtgt
     1501 caccgccagc ttcacctgcg aggcattcag cacgcacaag ctccagaccg tgaccgccgc
     1561 ggatgtgagc cggaggcttg aggagttgaa aaagttcacg agcttcgagg catgagccgc
     1621 cgagccgcga cccccgccgg cccgtcatag ggcgcgagga cccagggggg cggtccaggc
     1681 agccccgccc gcgccggaag gcggggggtg tgccatttcg cgcctggtga tctccgcggc
     1741 gttgtggcca gcccgccgca tcgtcacctc tgaggcgtgt tcatgcgctg gaaaaccacg
     1801 aatggacacg aatgagcacg aattttggag gcatcatgtc cgctgagctg aagcgccggg
     1861 agatcacccc tgggtgatgc cccccatccc tatcttctcc gcatggattc gtgtccattc
     1921 gtgtccattc gtggttcaac tgcatcgtta c
//
LOCUS       DAOMYJ010000466         1925 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Prosthecobacter sp. isolate AMR_MDS_5629
            NODE_23352_length_1925_cov_18.694118, whole genome shotgun
            sequence.
ACCESSION   DAOMYJ010000466 DAOMYJ010000000
VERSION     DAOMYJ010000466.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867850
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prosthecobacter sp. (wastewater metagenome)
  ORGANISM  Prosthecobacter sp.
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Verrucomicrobiaceae; Prosthecobacter.
REFERENCE   1  (bases 1 to 1925)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1925)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.30
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:34:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,742
            CDSs (total)                      :: 4,684
            Genes (coding)                    :: 4,676
            CDSs (with protein)               :: 4,676
            Genes (RNA)                       :: 58
            tRNAs                             :: 55
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1925
                     /organism="Prosthecobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23352_length_1925_cov_18.694118"
                     /isolate="AMR_MDS_5629"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1965333"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..271)
                     /locus_tag="PLP58_23210"
     CDS             complement(<1..271)
                     /locus_tag="PLP58_23210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012420422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase-related protein"
                     /protein_id="HRJ11507.1"
                     /translation="MSAKVPTAAEIRATFLDFFKEKQHTIVPSDNVGYTSRDGARIFT
                     NAGMNQFVPIFLGERSADVDTWPGVLHKGLPTRAADTQKCIRAGGK"
     gene            complement(365..1387)
                     /gene="lpxD"
                     /locus_tag="PLP58_23215"
     CDS             complement(365..1387)
                     /gene="lpxD"
                     /locus_tag="PLP58_23215"
                     /EC_number="2.3.1.191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009958026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-(3-hydroxymyristoyl)glucosamine
                     N-acyltransferase"
                     /protein_id="HRJ11508.1"
                     /translation="MHITLAELTELLQGTLLSGDPALAVTGFASLKEARAGDLSFYYD
                     TRYRERLEKTRAGAVLVPRDCANCPPHVACIAVDNPSRAFEKVVDTYGFHAAAFEAGV
                     HPSAVIAAGVKHDPAKVSIGANAVIEAGAELADGVEIGAGCYVGRDAVIGRDSKLFAS
                     VTIHEACELGERVILHSGVVIGADGFGYEFDKGRHRKVRQAGIVQIDNDVEIGAGTMV
                     DRARFGRTWIGEGTKIDNLVQIGHNAVIGRHCIIVACCAIAGSAVIGDYVVMAAQCGV
                     AGHVIIGSQITLGARSGVTKDIPDGPATYMGFPAMPVMDERRRLASINRIPALAARVK
                     ELEKRG"
     gene            complement(1447..1791)
                     /locus_tag="PLP58_23220"
     CDS             complement(1447..1791)
                     /locus_tag="PLP58_23220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009962312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HRJ11509.1"
                     /translation="MPETSPLDTHRPFYQCVRCGNCCRWPGDINITAAEAAAIAAFLG
                     IPEQDFIQNHTRLNANRTGLSIVDKPDGSCLFLEGVNTCLIQPVKPAQCSGFPNEWNF
                     PGWRDQCEAVEV"
BASE COUNT          302 a          562 c          707 g          354 t
ORIGIN      
        1 gcttgccgcc ggcgcggatg cacttctgcg tgtccgcggc gcgggtgggc aggcctttgt
       61 gcaagacgcc gggccaggtg tccacgtccg cgctgcgctc gccgaggaag atgggcacga
      121 actggttcat gcccgcgttc gtgaagatgc gcgcgccgtc gcggctggtg tagccgacgt
      181 tgtcgctggg cacgatggtg tgctgcttct ccttgaagaa atcgaggaag gtggcgcgga
      241 tttcggcggc ggtggggact ttggcggaca tgggcgggcg ggaaaaaggg gcggaaagta
      301 gcgggttgcg cggcaggcgc gatgattttt ttgaccgctg cccttcccga tgtccgcgcc
      361 ttcctcagcc gcgcttttcc agctccttga cgcgggcggc gagggcgggg atgcggttga
      421 tgctggcgag gcggcggcgc tcgtccatga ccggcatggc ggggaagccc atgtaggtgg
      481 cggggccgtc ggggatgtcc ttggtgacgc cgctgcgggc gccgagggtg atctggctgc
      541 caatgatgac atggccggcc acgccgcact gggcggccat gacgacataa tccccgatga
      601 cagcgctgcc agcgatggcg cagcaggcga cgatgatgca gtggcgtccg atgacggcgt
      661 tgtggccgat ctggacgagg ttgtctatct tggtgccctc gccgatccag gtgcggccaa
      721 agcgggcgcg gtccaccatg gttccggcgc cgatttcgac atcgttgtca atctgcacga
      781 tgccggcctg gcgcaccttg cggtgacgtc ccttgtcgaa ctcgtaaccg aaaccatccg
      841 cgccgatgac cacgccggag tgcaggatga cgcgctcgcc cagttcgcag gcctcgtgga
      901 tggtgacgct ggcaaagagc ttgctgtccc tgccgatgac ggcatcccgc ccgacgtagc
      961 agcccgcgcc gatctcgacg ccatcggcca gctccgcgcc cgcctcgatg acagcgttgg
     1021 cgccgatgga gactttggcg gggtcgtgtt tcactcccgc cgcgatgacg gcggaggggt
     1081 ggacccccgc ctcaaaggcg gcggcgtgga agccgtaggt gtccaccact ttttcaaagg
     1141 cgcgggaggg attgtccacg gcgatgcagg ccacatgcgg ggggcagttc gcgcagtcac
     1201 gcggcacgag cacggctccg gcccgcgtct tctccagccg ctcgcggtag cgcgtgtcat
     1261 aatagaagct caagtcgccg gcgcgggcct ctttgagcga tgcgaatccg gtgacggcca
     1321 gcgcgggatc gccggaaagg agcgtgcctt gaaggagttc cgtgagttcg gcgagtgtga
     1381 tgtgcatggg gcgggctttt tgtcacagat gacacggaaa gcaacgcttg aattcggcgg
     1441 aaggactcac acctccaccg cctcgcattg atcccgccag ccggggaagt tccattcatt
     1501 cgggaaaccg gagcactggg cgggcttgac gggctggatg aggcaggtgt tcacgccttc
     1561 gagaaagagg cagcttccat ccggcttgtc cacgatggag agaccggtgc ggttggcgtt
     1621 gaggcgcgtg tggttttgga tgaagtcctg ctccgggatg ccgagaaagg cggcgatggc
     1681 ggcggcttcg gcggcggtga tgttgatgtc gcccggccag cggcagcagt ttccgcagcg
     1741 cacgcactgg taaaaaggtc tgtgtgtgtc aagcggggag gtttcgggca tgtgaacttt
     1801 attgttcgcg cgcggcgcgc gccttgctca tgattggcgc atgttcaagg tcatgcaacg
     1861 catggatgac ggcgcgccgc cgcacacgca gggcggcgcg cgggtgtgcg cacccgtcac
     1921 gcggc
//
LOCUS       DAOMYJ010000467         1923 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Prosthecobacter sp. isolate AMR_MDS_5629
            NODE_23384_length_1923_cov_17.534797, whole genome shotgun
            sequence.
ACCESSION   DAOMYJ010000467 DAOMYJ010000000
VERSION     DAOMYJ010000467.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867850
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prosthecobacter sp. (wastewater metagenome)
  ORGANISM  Prosthecobacter sp.
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Verrucomicrobiaceae; Prosthecobacter.
REFERENCE   1  (bases 1 to 1923)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1923)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.30
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:34:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,742
            CDSs (total)                      :: 4,684
            Genes (coding)                    :: 4,676
            CDSs (with protein)               :: 4,676
            Genes (RNA)                       :: 58
            tRNAs                             :: 55
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1923
                     /organism="Prosthecobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23384_length_1923_cov_17.534797"
                     /isolate="AMR_MDS_5629"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1965333"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(149..937)
                     /locus_tag="PLP58_23225"
     CDS             complement(149..937)
                     /locus_tag="PLP58_23225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009963768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HRJ11510.1"
                     /translation="MTPPDSAVVPVRCEFVRGRNCLLVRGQFSPLYMDYYLHLMQHGL
                     KLADDPDQMLKDALATLALHLASRPQDEGCAWTVNFHDPLMNLFVTGGSRPGRVTGRV
                     FTEDVKDSGKNIFIAQTTRPHMQPRQSMIEFAGADIPAAVEQFYTQSEQRPTRVFRLP
                     DEEFAQISAEPDADLDWLASLTPEQFPGLGETETLSLLETRGYIFECGCTPDRLYPLL
                     TRLPDADLDYIFEDGFATITCPRCAAVFRAPKSAFEAWKARQPA"
     gene            complement(934..1647)
                     /locus_tag="PLP58_23230"
     CDS             complement(934..1647)
                     /locus_tag="PLP58_23230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009964102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1080 domain-containing protein"
                     /protein_id="HRJ11511.1"
                     /translation="MKKVTHLATLAILGLLASCGPKEEFTGKPADLTPTGTPAAKPDS
                     EKGWMTIFNGNDLAGWKDSAETPDSFIVEDGALKVCNGRSHLFYTGPTGDASFKNFEF
                     KATVKHMPGSNSGIYIHTKFQEKGWPDTGYECQVNSTGHKDPKKTGGLYAVKDVLDTA
                     PVGDDEWFEYFIRVQDKKIIIKINGQTTTEWTEPDGWDPATALKNMPGRKLGEGTIAI
                     QGHDPQSVVYYKDIKVRKL"
     gene            complement(1679..>1923)
                     /locus_tag="PLP58_23235"
     CDS             complement(1679..>1923)
                     /locus_tag="PLP58_23235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ11512.1"
                     /translation="AALAWTVAALAWHGSPGLLLLHACLWTPLVVIAAGLGLERALEH
                     WPRLRWPVALLLAAFLAAQVTRNWAFLQQVAQEARL"
BASE COUNT          295 a          534 c          725 g          369 t
ORIGIN      
        1 aggtcccccg ctcgcagtcg gtccggggac cgtcctggcg ggtgagcagg ccggtgaggc
       61 ggttggcggc ggcgggcagc actttgatga gcttttcgtt gccggtcttg taggcggggg
      121 cgttcatgct cggaggaata cgaacggctc aggcgggctg gcgcgctttc caggcttcga
      181 aggcgctttt gggcgcgcgg aagacggcgg cgcagcgcgg gcaggtgatg gtggcgaaac
      241 cgtcctcgaa aatgtagtcg aggtcggcgt caggcagccg ggtgagcagc gggtagagcc
      301 ggtccggggt gcagccgcac tcgaagatgt agccgcgcgt ttcgagcagg gacagggttt
      361 cggtttcgcc gaggccgggg aattgctccg gggtgaggga ggcgagccag tcgaggtcgg
      421 catccggctc ggcggaaatc tgcgcgaact cctcgtcggg caggcggaag acacgggtgg
      481 ggcgctgctc gctctgagtg taaaactgct cgactgcggc cgggatgtca gcaccggcaa
      541 actcgatcat gctctggcgc ggctgcatgt gcggccgcgt ggtctgggcg atgaagatat
      601 tttttccgct gtccttcaca tcctcggtga agacgcgccc ggtgactctg ccggggcggc
      661 tgccgccggt gacgaagagg ttcatgagcg gatcgtggaa gttcaccgtc caggcgcagc
      721 cctcgtcctg cggacgggag gccaggtgca gggcgagggt ggccagggcg tccttgagca
      781 tctggtcggg atcgtcggcc agcttcaggc cgtgctgcat gaggtgcagg tagtagtcca
      841 tgtagagcgg gctgaactgc ccgcgcacaa gcaggcagtt gcgcccgcgc acgaactcgc
      901 agcggacggg gacgacggct gaatcgggcg gcgtcacagc ttgcggactt tgatgtcctt
      961 gtaataaacg acgctctgcg ggtcgtggcc ctggatggcg atggtgccct ctccgagctt
     1021 gcgtccgggc atgtttttca gcgcggtggc gggatcccag ccgtcgggct cggtccactc
     1081 ggtggtggtc tggccgttga tcttgatgat gatctttttg tcctggacgc ggatgaagta
     1141 ctcgaaccat tcgtcatcgc cgacgggcgc ggtgtcgagc acgtccttga cggcataaag
     1201 gccgccggtc tttttgggat ccttgtggcc ggtgctgttg acctggcatt catagccggt
     1261 gtcgggccag cctttttcct ggaacttggt gtggatgtag atgccggagt tggagcctgg
     1321 catgtgcttg acggtggctt tgaactcgaa gttcttgaag ctggcgtcgc ccgtcgggcc
     1381 ggtataaaaa aggtgggagc ggccgttgca gaccttgagc gcgccgtcct cgacgatgaa
     1441 ggaatcgggc gtttccgcgc tgtccttcca gccggcgagg tcgttgccgt tgaagatggt
     1501 catccagcct ttctcactgt ccggcttcgc ggcgggtgtg ccggtcgggg tgagatcggc
     1561 gggcttgccg gtgaattctt ccttggggcc gcaggaggcg agcaagccca ggatggcgag
     1621 ggtggcgaga tgcgtgactt ttttcatggg aggccgtttt tacacgggaa ggcgggcatt
     1681 acaagcgcgc ttcctgcgcc acctgctgga ggaaggccca gttccgcgtg acctgcgcgg
     1741 cgaggaaggc ggccagcagc aatgccaccg gccagcgcag gcgcggccag tgctccagcg
     1801 cgcgctccag gccaaggccg gcggcgatga ccaccagcgg cgtccacagg catgcgtgca
     1861 gcagcaggag gcctggcgag ccgtgccagg caagggcggc cacggtccat gccagcgcgg
     1921 cga
//
LOCUS       DAOMYJ010000468         1922 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Prosthecobacter sp. isolate AMR_MDS_5629
            NODE_23392_length_1922_cov_19.163899, whole genome shotgun
            sequence.
ACCESSION   DAOMYJ010000468 DAOMYJ010000000
VERSION     DAOMYJ010000468.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867850
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prosthecobacter sp. (wastewater metagenome)
  ORGANISM  Prosthecobacter sp.
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Verrucomicrobiaceae; Prosthecobacter.
REFERENCE   1  (bases 1 to 1922)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1922)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.30
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:34:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,742
            CDSs (total)                      :: 4,684
            Genes (coding)                    :: 4,676
            CDSs (with protein)               :: 4,676
            Genes (RNA)                       :: 58
            tRNAs                             :: 55
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1922
                     /organism="Prosthecobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23392_length_1922_cov_19.163899"
                     /isolate="AMR_MDS_5629"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1965333"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..103)
                     /locus_tag="PLP58_23240"
     CDS             complement(<1..103)
                     /locus_tag="PLP58_23240"
                     /inference="COORDINATES: protein motif:HMM:NF013543.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ11513.1"
                     /translation="MGLKVVELKPIDGFAAETAAATVIEVASRLGIPV"
     gene            308..>1922
                     /locus_tag="PLP58_23245"
     CDS             308..>1922
                     /locus_tag="PLP58_23245"
                     /inference="COORDINATES: protein motif:HMM:NF018983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016977 - chitosanase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 75 protein"
                     /protein_id="HRJ11514.1"
                     /translation="MRDSTPTTPLSDDPTPPPRGPGVIASLSRLLVLVIIAMLLVLPF
                     TPFAGRIKTALKEIATAWKEERTQVVTKEVEKIVQAPPREIVKEVVKEVVKEVPAPPP
                     PLPDAFIPRKEVDVATLYNGITIKSNLLMEQGTYASLERLDPEAYKAEFQLTVRVPKA
                     NQSLQELSRINPALPSLLPGLDAMIPASRVSGFYHKLYENKTRGIQRDITRLNRVLDR
                     HNFFDCETIIELTHPVSNRKALLIQSEMDVVADGSDGDRMPTLDEYIYMSDYYQPFTS
                     YAWGKRTKTPNPLLSRWEARLKKAREEYAVKGLSAERNAYLRAQISQLDLEIKDMKAR
                     SSLIAEKDPFVVISLLFRPYADSNPHTPKIGDYAVVVHGGKIYPAICGDYGPTQKMGE
                     ASLLMAKTINDKATPYRRPESELKVTYVIFPGTAEKPFGPPDLDRWHERCAEYLEEIG
                     TSNAAAVMHRWEDPFKKPDPPAADPIANAIGLVPSMIIELAICTMYKGLPPLEEMAFF
                     NGAGNSSPMMSSAMAIEASLVNGCNSNCKK"
BASE COUNT          392 a          675 c          547 g          308 t
ORIGIN      
        1 aaacgggaat accgagccgg ctggcgacct cgatcaccgt cgcggcggcc gtttcggcgg
       61 cgaagccatc gatcggcttg agttccacca ccttcaggcc catcgttttc acgatccgcc
      121 agccgccgga ggcggtggtg gcggctgccc tgcggacttt ggaaaatggc gggccgctgg
      181 tgtttccggg cgcgggcgtg aaggcggcgg cctgcctgtt ccgccatctg ccgcgtttcg
      241 tgctgcacag gctcttgcgg gcacggcacg cgcgggccaa ggtttccgca gcacccccgc
      301 cgccgaaatg agagactcca cgcccacgac tccgctttcc gatgatccaa cgccgccgcc
      361 gagggggccg ggcgtcatcg cctcgctcag ccggctgctg gtgctggtga tcatcgccat
      421 gctgctggtg ctgcctttta cgccgttcgc gggcaggatc aaaaccgcgc tcaaagagat
      481 cgccaccgcg tggaaggagg agcgcaccca ggtcgtcacg aaggaggtgg agaagatcgt
      541 ccaggcgccg ccgagggaga tcgtcaaaga agtggtcaag gaggtcgtca aggaagtgcc
      601 tgcgccgccg ccgcccctgc cggatgcgtt catcccccgc aaggaggtgg acgtggccac
      661 gctgtacaac ggcatcacca tcaagagcaa cctgcttatg gagcagggca cctatgcgag
      721 cctggagcgg ctcgaccccg aagcctacaa ggccgagttc caactcaccg tgcgcgtgcc
      781 gaaggccaac caaagcctcc aggagctttc ccgcatcaat cccgccctgc cttcgctgct
      841 gcccggtctt gatgccatga tccccgcctc cagggtctcc ggcttctacc acaagctcta
      901 cgagaacaaa acccgcggca tccagcgcga catcacccgc ctcaaccgcg tgctcgaccg
      961 ccacaatttc ttcgactgcg agaccatcat cgagctcacc caccccgtct ccaatcgcaa
     1021 agccctgctc atccagtccg agatggacgt ggtggctgac ggctccgacg gcgaccggat
     1081 gccgaccctg gacgagtaca tctacatgtc cgactactac cagcccttca ccagctacgc
     1141 ctggggcaag cgcaccaaga cgccgaaccc gctgctctcc aggtgggagg cgcggctgaa
     1201 aaaggccagg gaagaatacg ccgtcaaggg cctcagcgcc gagcgcaacg cctacttgcg
     1261 cgcccagatc agccagctcg accttgagat caaggacatg aaggcgcgca gcagcctcat
     1321 cgccgagaaa gaccccttcg tcgtcatctc cctgctcttc cgcccatacg ccgacagcaa
     1381 cccgcacacg ccgaaaatcg gcgattacgc tgtcgtggtg catggcggca aaatctaccc
     1441 cgccatctgc ggcgactacg gccccacgca aaaaatgggc gaggcctccc tgctcatggc
     1501 aaagaccatc aacgacaaag ccacccccta ccgccgtccg gagagcgagc tcaaagtcac
     1561 ctatgtcatc tttccaggca ccgccgaaaa gcccttcggc ccgcccgatc tcgaccgctg
     1621 gcacgagcgc tgcgccgagt atctggagga gatcggcacg tcaaacgccg ccgccgtcat
     1681 gcaccgctgg gaggaccctt tcaaaaagcc cgatccgccc gccgccgacc ccatcgccaa
     1741 cgcaatcgga ctcgtgccat cgatgatcat cgaactggcc atttgcacga tgtacaaagg
     1801 attgcctcca ctggaggaga tggccttctt caacggagcc ggaaattcat cgccgatgat
     1861 gtcctcggcg atggcgatcg aggcgtcttt ggtcaacggt tgcaactcca actgcaaaaa
     1921 gc
//
LOCUS       DAOMYJ010000469         1921 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Prosthecobacter sp. isolate AMR_MDS_5629
            NODE_23405_length_1921_cov_20.465166, whole genome shotgun
            sequence.
ACCESSION   DAOMYJ010000469 DAOMYJ010000000
VERSION     DAOMYJ010000469.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867850
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prosthecobacter sp. (wastewater metagenome)
  ORGANISM  Prosthecobacter sp.
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Verrucomicrobiaceae; Prosthecobacter.
REFERENCE   1  (bases 1 to 1921)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1921)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.30
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:34:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,742
            CDSs (total)                      :: 4,684
            Genes (coding)                    :: 4,676
            CDSs (with protein)               :: 4,676
            Genes (RNA)                       :: 58
            tRNAs                             :: 55
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1921
                     /organism="Prosthecobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23405_length_1921_cov_20.465166"
                     /isolate="AMR_MDS_5629"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1965333"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..378)
                     /locus_tag="PLP58_23250"
     CDS             complement(<1..378)
                     /locus_tag="PLP58_23250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009965584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ11515.1"
                     /translation="MPSATRLLPALLLAALPLSAADEAKVEFNRHIRPLLAAKCYACH
                     GPDEDKREAGLRLDIREEAVKEAIRPGKPEASEFWHRISTADPDDVMPPPSSPKPLTG
                     AERALLRRWIAQGAEYQQHWSFLP"
     gene            complement(385..1833)
                     /locus_tag="PLP58_23255"
     CDS             complement(385..1833)
                     /locus_tag="PLP58_23255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009961543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1501 domain-containing protein"
                     /protein_id="HRJ11516.1"
                     /translation="MPAFQLCSRRHFLHASGLGPGTVALASLLHREGLLAAPVKPATF
                     GEERHDLTSKKPHFEPRAKAMISLFMMGGPSQMDLFDPKPMLTRHHGRKFPGEVKYDN
                     LAQASSKCFGSPWKFQKHGQCGMEISELLPHLSKVVDDITLIRSMTSGVSNHAQGLYA
                     MNAGRVTAGRPALGSWLTYGLGSETDDLPAYMVLSHPGGLPTFQQEHFTNGWLPALYQ
                     GTLVRAAEPRILNLDPPPALAGAPQQRQLDLLRAFNEEHLAARPGELDLQARIASYEL
                     AANMQTAAKEALDISGEPAHILKLYGADNPVTKDYATRCIIARRLVERGVRYVQVLNA
                     GQSWDQHSALVTSLPKNCAATDQPSAALLIDLKQRGLLDSTVVHWGGEMGRLPVLQND
                     AGPEKWGRDHNTYGFSMWVAGGGFKKGHVHGQTDDFGHKAVLDEVRHFDYHATLLHLF
                     GLDHTRLTYKRNGLAASLTDNQGAKVITPLLA"
BASE COUNT          258 a          526 c          817 g          320 t
ORIGIN      
        1 ggggaggaag gaccagtgct gctggtattc ggctccctgg gcgatccagc ggcggaggag
       61 ggcgcgctcg gcgccggtga ggggcttggg agaggatggc ggcggcatga cgtcatcggg
      121 gtcggcggtg ctgatgcggt gccagaactc gctggcctcg ggtttgccgg ggcggatggc
      181 ttccttgacg gcctcctcgc ggatgtcgag gcggaggccg gcctcgcgct tgtcctcgtc
      241 gggcccgtgg caggcgtagc acttggcggc gaggaggggg cggatgtggc ggttgaactc
      301 cactttggcc tcgtccgccg cgctgagggg cagggcggcg aggaggagtg cggggaggag
      361 acgcgtcgcg gacggcatga ggggtcaggc caggaggggt gtgatgactt tggcgccctg
      421 gttgtcggtg agggaggcgg cgaggccgtt gcgcttgtag gtgaggcggg tgtggtcgag
      481 gccgaagagg tggaggaggg tggcgtggta gtcgaagtgg cgcacctcgt cgaggacggc
      541 cttgtggccg aagtcgtcgg tctggccgtg gacatggcct ttcttgaagc cgccgccggc
      601 gacccacatg ctgaagccgt aggtgttgtg gtcgcgcccc catttctccg gcccggcgtc
      661 attctggagg acgggcaggc ggcccatctc gccgccccag tggacgacgg tgctgtcgag
      721 caggccgcgc tgcttgaggt caatgagcag ggcggcgctg ggctggtcgg tggcggcgca
      781 gttcttgggc agggaggtga cgagggcgct gtgctggtcc caggactggc cggcattgag
      841 cacctggacg taacgcacgc cgcgctcgac gaggcggcgg gcgatgatgc agcgggtggc
      901 gtagtccttg gtgacggggt tgtccgcgcc gtagagcttg aggatgtggg cgggctcgcc
      961 ggagatgtcg agggcctcct tggcggcggt ctgcatgttg gcggcgagct cgtagctggc
     1021 gatgcgggcc tggaggtcca gctcgccggg gcgggcggcg aggtgctcct cgttgaaggc
     1081 cctgagcagg tcgagctgcc tctgctgcgg ggcgccggcg agtgcggggg gaggatcgag
     1141 attgaggatg cgcggctcgg cggcgcggac gagggtgccc tggtagaggg cgggcagcca
     1201 gccgttggtg aagtgctcct gctggaaggt gggcaggccg ccggggtggg agagcaccat
     1261 gtaggcgggc aggtcgtccg tctcgctgcc gaggccgtag gtgagccagg agccgagggc
     1321 gggcctgccg gcggtgacgc gcccggcgtt catggcgtag aggccctggg cgtggttgct
     1381 gacgccggag gtcatggagc ggatgagggt gatgtcgtcc acgaccttgg agaggtgcgg
     1441 gagcagctcg ctgatctcca tgccgcactg gccatgcttt tggaacttcc agggggagcc
     1501 aaagcacttg gaggaggcct gggcgaggtt gtcgtatttg acctcgccgg ggaacttccg
     1561 cccgtggtgc ctggtgagca tgggcttggg gtcgaagagg tccatctggg aggggccgcc
     1621 catcatgaag agcgagatca tggccttcgc cctcggctcg aagtgcggct ttttcgaggt
     1681 caggtcatgc ctctcctccc cgaaggtcgc cggcttcacc ggcgcggcca gcagcccctc
     1741 ccggtgcaga agcgaggcga gcgccaccgt gccgggaccg aggccgctgg catggaggaa
     1801 gtgacgacgt gagcaaagct ggaaggcggg catgagcgcc cgtatctacg cggcgcggac
     1861 gcgcattctc acaggcggcg cgtgatccgc gggcggagcg cgccgccgga ggcccgcccg
     1921 c
//
LOCUS       DAOMYJ010000470         1910 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Prosthecobacter sp. isolate AMR_MDS_5629
            NODE_23600_length_1910_cov_23.832345, whole genome shotgun
            sequence.
ACCESSION   DAOMYJ010000470 DAOMYJ010000000
VERSION     DAOMYJ010000470.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867850
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prosthecobacter sp. (wastewater metagenome)
  ORGANISM  Prosthecobacter sp.
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Verrucomicrobiaceae; Prosthecobacter.
REFERENCE   1  (bases 1 to 1910)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1910)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.30
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:34:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,742
            CDSs (total)                      :: 4,684
            Genes (coding)                    :: 4,676
            CDSs (with protein)               :: 4,676
            Genes (RNA)                       :: 58
            tRNAs                             :: 55
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1910
                     /organism="Prosthecobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23600_length_1910_cov_23.832345"
                     /isolate="AMR_MDS_5629"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1965333"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(172..522)
                     /locus_tag="PLP58_23260"
     CDS             complement(172..522)
                     /locus_tag="PLP58_23260"
                     /inference="COORDINATES: protein motif:HMM:NF006573.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter subunit C"
                     /protein_id="HRJ11517.1"
                     /translation="METLLSILTGLLFALGVYLLLRRSLVQVLLGIMFLSHAANLVIF
                     VSGGLRRDRAPMIAEDGTVPAAGYADPLPQALILTAIVISFGLLAFALALVMRAQSRL
                     GTDDVEELTREDAA"
     gene            complement(583..1533)
                     /locus_tag="PLP58_23265"
     CDS             complement(583..1533)
                     /locus_tag="PLP58_23265"
                     /inference="COORDINATES: protein motif:HMM:NF015338.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HRJ11518.1"
                     /translation="MPSTRSFFSASIRLDGLLDTCICLGLAASWLGLFGGWHWALDLC
                     SHFRWQQLGVSLLALVWALWRGRRFLRHVSMLTLLLNAALITQHTWPRPAKGTASGAP
                     LNVVSLNLLLSNPHKQKVLDYLLSCDADMIYLSEVHGEWAAALEPLKIRYPHHLLETN
                     HGAFGAAFFSRVPVRSMRVRNFGAENSVCIEVLAAHEGREFHLIGTHPPPPMGAGFAR
                     VQRAEFEGIARHTAALGVPVLLVGDFNATPWCHAFRLLEKGGLRASGPAWPPTWSTRS
                     IFALPIDHAMTTAPLRIIQRGIGPDVGSDHRPVEITLGWE"
     gene            complement(1604..>1910)
                     /locus_tag="PLP58_23270"
     CDS             complement(1604..>1910)
                     /locus_tag="PLP58_23270"
                     /inference="COORDINATES: protein motif:HMM:NF013760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HRJ11519.1"
                     /translation="NYLNVFATISPMIGLFGTVSGMITAFNQLKLGQSEPADLAGGIG
                     EAMLTTAGGLIVGIPAMFFFFFFRNKLMGITADIQRHVSFLIGVLSGEVKLSGGEGE"
BASE COUNT          383 a          628 c          641 g          258 t
ORIGIN      
        1 catggatgcc gccctcatgc gtgcccgcca ccagcgccgc gcccgccgcg cacagccccg
       61 ccgtgaccag cagcgtgatg acacgatgca gcgcgtaaca ccgccggccc gccagcgcct
      121 ggagcgcggc gccgagaaac ggcaggagaa tcggtgccac caccgccgcc gtcatgccgc
      181 gtcctcccgc gtcagctcct ccacatcatc cgtgcccaga cgcgactgcg cgcgcatcac
      241 cagcgccagc gcgaaggcca gcaggccgaa actgatgaca atggccgtga ggatgagcgc
      301 ctgcggcaaa gggtcggcgt aacccgccgc cggcaccgtg ccatcctccg cgatcatcgg
      361 cgccctgtca cggcgcagcc cgccgctgac aaaaatgacc agattcgccg catggctcag
      421 aaacatgatg cccagcagca cctgaacgag gctgcgccgc aaaagcaaat acacaccgag
      481 ggcaaaaagc agcccggtga ggatggagag gagggtttcc atgcgcgggc atcttgaaac
      541 agccggggcg gactggcaag gcggggatgc cacgctgccc gctcactccc agcccagcgt
      601 gatctccacc ggccggtggt cggagccgac atcggggccg atgccgcgct ggatgatccg
      661 cagtggcgcg gtggtcatgg catggtctat gggcagggcg aagatggagc gcgtgctcca
      721 cgtcggcggc caggccggac ctgaggcgcg cagcccgccc ttttccagca ggcggaaggc
      781 atggcaccag ggcgtggcat tgaagtcccc caccagcagc accggcacgc cgagcgccgc
      841 cgtgtggcgg gcgatgccct caaactccgc ccgctgcaca cgcgcaaatc ccgctcccat
      901 cggcggcggc ggatgcgtgc cgatgagatg aaactcccgg ccctcatgcg cggccagcac
      961 ctcgatgcag accgagttct ccgcgccaaa attacgcacc cgcatggacc ggaccggcac
     1021 ccggctgaaa aacgccgcgc caaacgcgcc atgattcgtc tccagcaaat gatgcggata
     1081 ccggatcttc agcggctcca gagcggccgc ccattcgcca tgcacctcgg acaggtagat
     1141 catgtccgca tcgcaggaga gcaggtaatc cagcaccttt tgcttgtggg ggttgctgag
     1201 cagcagattc aggctgacga catttagcgg cgcgccgctc gccgtgccct tggcgggtct
     1261 gggccaggtg tgctgggtga tgagcgcggc attcagcagc agtgtcagca tcgagacatg
     1321 gcgcaggaag cggcgcccgc gccaaagcgc ccacacaagg gccagcaggc tgacgccgag
     1381 ctgctgccag cggaagtggc tgcaaagatc cagcgcccaa tgccagcctc cgaacaagcc
     1441 gagccaggag gcggccagcc cgaggcagat gcaggtgtcg agcaaaccgt cgagccggat
     1501 gctggcggag aaaaaggagc gcgtcgaagg cattgaagac tgaacaggcg gggcgtgtcg
     1561 tttctccggc ggggcaaatc cacccaactt gggactcctg acctcactcc ccttccccgc
     1621 cggagagttt cacctcgccg gagaggacgc cgatgaggaa ggagacatgg cgctggatgt
     1681 cggcggtgat gcccatgagc ttgttgcgga agaagaagaa aaagaacatg gccgggatgc
     1741 cgacgatgag gccgccggcg gtggtgagca tggcctcgcc gatgccgccg gcgaggtcgg
     1801 cgggctcgct ctggccgagc ttgagctggt tgaaggcggt gatcatgccg ctgacggtgc
     1861 cgaagaggcc gatcatgggc gagatggtgg cgaagacatt gaggtagttc
//
LOCUS       DAOMYJ010000471         1895 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Prosthecobacter sp. isolate AMR_MDS_5629
            NODE_23874_length_1895_cov_23.590217, whole genome shotgun
            sequence.
ACCESSION   DAOMYJ010000471 DAOMYJ010000000
VERSION     DAOMYJ010000471.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867850
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prosthecobacter sp. (wastewater metagenome)
  ORGANISM  Prosthecobacter sp.
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Verrucomicrobiaceae; Prosthecobacter.
REFERENCE   1  (bases 1 to 1895)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1895)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.30
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:34:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,742
            CDSs (total)                      :: 4,684
            Genes (coding)                    :: 4,676
            CDSs (with protein)               :: 4,676
            Genes (RNA)                       :: 58
            tRNAs                             :: 55
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1895
                     /organism="Prosthecobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23874_length_1895_cov_23.590217"
                     /isolate="AMR_MDS_5629"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1965333"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..808)
                     /locus_tag="PLP58_23275"
     CDS             complement(<1..808)
                     /locus_tag="PLP58_23275"
                     /inference="COORDINATES: protein motif:HMM:NF024867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HRJ11520.1"
                     /translation="MRLHSITVRHYRLHKECRVEFDDALTLVGGPNESGKSTLIEAAH
                     RAFFLNAKGNTEAHRAMQSVIHDGRPEVEIEFTAHGRRHTLIKQFKGANGVTRLTCHG
                     GQTWTHEEAEEKLAELTGAPHVKANRIHEPWAHLWIMQGRSGDNPVAQAGSEYDNLMR
                     HLQTGGGAAVMQSGVDARLAACFAEWMEQAFTQGGTPRSQSDAGRAEQELREAETVEL
                     QARQVLETLDTALADHAGAAARLAEAEAALRTLHEEQARLDERAARIAALR"
     gene            complement(853..>1895)
                     /locus_tag="PLP58_23280"
     CDS             complement(853..>1895)
                     /locus_tag="PLP58_23280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="DNA repair exonuclease"
                     /protein_id="HRJ11521.1"
                     /translation="AALDLREHETGLHSKRAPRPDKATVSAACSAIGALQLPVYAIPG
                     NHDHGGPDSLWEQPFFLQEKQALAPNLHVLLKAEPLVLDECVILPSPLTRRHDTQDAT
                     AWVRALDFSVFADKPRIGLAHGSVQDFGPAGDDDEEDEGGCSNLLDLSRLPLAELDYL
                     ALGDWHGMRQAGPRAWYSGTPEPDRFPKNNAPGHVLAVTAARGVEPQITPVPTARLRW
                     HDMEMDFASDADLPLLEDKLAALTQSRAGEDLVRLTLRGSLGMEAMAGLESALDTWRA
                     RLLRLRLHNHVRLTPAAAEIEALTRRADAPLTARIAARLLEESQSADAAVSGTALAAL
                     RELHAAMAVTPS"
BASE COUNT          303 a          599 c          665 g          328 t
ORIGIN      
        1 ggcgcagggc ggcgatgcgc gcggcacgct cgtcgagcct cgcctgctcc tcgtgcagag
       61 tcctgagagc cgcctccgcc tcggcaagcc gcgccgccgc gcctgcatgg tccgcaagcg
      121 cggtgtccaa ggtctcaagg acctgccgcg cctgcagttc gacagtttca gcctcgcgta
      181 attcttgttc ggcgcgcccg gcatccgact gcgaccttgg ggtgccgcct tgagtgaaag
      241 cctgctccat ccattcggca aaacaggccg cgagccgcgc gtccacaccg gactgcatca
      301 cggccgcgcc tcccccggtc tggagatgac gcatgaggtt gtcatactcg ctcccagctt
      361 gtgcgacagg gttgtcaccg gacctgccct gcatgatcca gagatgcgcc cagggctcgt
      421 ggatgcggtt tgccttgacg tgcggggcgc cggtcagttc ggcgagcttt tcctcggcct
      481 cctcatgggt ccaggtctgg ccgccgtggc aggtcaggcg cgtgacgcca ttggcgccct
      541 tgaactgctt gatcaaggtg tggcgcctgc cgtgcgcggt gaactcgatc tcgacctccg
      601 gcctgccatc atgaatgacc gactgcatgg cccggtgcgc ttctgtattg cccttggcgt
      661 tgaggaaaaa cgcgcggtgc gcggcctcga tgagcgtgct cttcccggat tcgttcggtc
      721 cgccgaccag tgtcagcgcg tcatcaaact cgactctgca ctccttgtgc aggcggtaat
      781 gacgaacggt aatggagtgc aggcgcatgg gacggaggat cgaggattat gaaattcaac
      841 ggcatgagcg aatcaggacg gcgtgacggc catggcggcg tgcagttcgc gcagggcggc
      901 aagggcggtc cctgagacgg cggcatcggc gctttgggat tcctccagca gacgggcggc
      961 gatgcgcgcg gtgagcgggg cgtccgcgcg gcgtgtgagc gcctcgatct cggcggcggc
     1021 gggtgttaga cggacgtggt tgtggaggcg caggcgcagc aggcgcgcgc gccaggtatc
     1081 cagcgcgctt tccaatcccg ccatcgcctc catgccaagg ctgccgcgca gggtcaggcg
     1141 gacgaggtcc tcaccggcgc gggactgggt gagggcggcc agcttatcct caagcaacgg
     1201 caggtcggcg tcggaggcga aatccatctc catgtcatgc cagcgcaagc gcgcggtcgg
     1261 cacgggggtg atttgcggtt ccactccgcg cgccgcagtg acggcgagca catggccggg
     1321 cgcgttgttt ttcgggaaac gatcgggctc cggggtgccc gaataccacg cgcgcgggcc
     1381 ggcctgcctc atgccgtgcc agtctccgag cgccagataa tccagctccg cgagcggcag
     1441 acgggagagg tcgagcaggt tcgagcaacc gccctcatcc tcctcatcgt cgtcccctgc
     1501 ggggccgaaa tcctgcacgc tgccatgcgc cagcccgatg cgcggcttgt ccgcgaagac
     1561 ggagaaatcc agcgcgcgca cccaggcggt ggcgtcctgc gtgtcatgac gacgtgtcag
     1621 cggggagggc agaatgacgc actcatcgag caccagcggc tcagccttca aaagcacatg
     1681 caagttgggc gccagcgcct gtttttcctg gaggaaaaaa ggctgttccc acaatgaatc
     1741 cgggccgccg tggtcatggt tgccggggat ggcataaacg ggaagctgga gcgcgcctat
     1801 ggccgagcag gcggcggaga cggtggcctt gtcgggccgg ggagctcgct tggaatgaag
     1861 gccggtttcg tgctcacgca ggtcgagggc ggcgg
//
LOCUS       DAOMYJ010000472         1887 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Prosthecobacter sp. isolate AMR_MDS_5629
            NODE_24033_length_1887_cov_15.800764, whole genome shotgun
            sequence.
ACCESSION   DAOMYJ010000472 DAOMYJ010000000
VERSION     DAOMYJ010000472.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867850
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prosthecobacter sp. (wastewater metagenome)
  ORGANISM  Prosthecobacter sp.
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Verrucomicrobiaceae; Prosthecobacter.
REFERENCE   1  (bases 1 to 1887)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1887)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.30
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:34:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,742
            CDSs (total)                      :: 4,684
            Genes (coding)                    :: 4,676
            CDSs (with protein)               :: 4,676
            Genes (RNA)                       :: 58
            tRNAs                             :: 55
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1887
                     /organism="Prosthecobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24033_length_1887_cov_15.800764"
                     /isolate="AMR_MDS_5629"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1965333"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..707
                     /locus_tag="PLP58_23285"
     CDS             <1..707
                     /locus_tag="PLP58_23285"
                     /inference="COORDINATES: protein motif:HMM:NF014325.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="TrkA family potassium uptake protein"
                     /protein_id="HRJ11522.1"
                     /translation="LPTPMRFCIIGLGSFGTNLARQLARDGHEVVGVDNEPAHTDAMR
                     EEIEFLFTADCSDITVFQDIPAADCDAVIIAIGKDFEASLSIAANVQQTGARRIICRV
                     INPLHARLLKLLKIDELLIPEAMAAAWLARTLKAPDVLNSFQVGQGHEIVEVILPPGM
                     AGRTLVEMDLRGRYDLNLVTILRRKNITTLSSSAPQIEKIIGIPEPGRVFEKEDILLL
                     FGTEKNVRRLLADHAA"
     gene            complement(1001..1657)
                     /locus_tag="PLP58_23290"
     CDS             complement(1001..1657)
                     /locus_tag="PLP58_23290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ11523.1"
                     /translation="MKLALCIVLSILAYESACVLNAAASGGIPMENGISYGVFFGSLI
                     FVFTYPCWLAGGFVLRWLAELVLRRELPMRQYLPFWGHLPISMLLLATMPGPPTPEGL
                     IREYVADECPASLGDFGVWMQRGFGHQTLIMSYSLDPAEFPLLLKRHEFKESHDAAGV
                     DLHPLDFIIRRVPGIPIQLPDAPLVWRYSHFKDQTHLQQVNHYLTRDKARVITYISYN
                     "
BASE COUNT          358 a          695 c          558 g          276 t
ORIGIN      
        1 acctccccac ccccatgcgc ttctgcatca tcggcctcgg ctccttcggc accaacctcg
       61 cacgccagct cgcccgcgac ggccatgagg tcgtcggcgt ggacaacgag cccgcccaca
      121 ccgacgccat gcgcgaggag atcgagttcc tcttcaccgc cgactgctcc gacatcaccg
      181 tcttccagga catccccgcc gccgactgcg acgccgtcat catcgccatc ggcaaggact
      241 tcgaggcctc cctctccatc gccgccaacg tccagcagac cggcgcccgc cgcatcatct
      301 gccgcgtcat caaccccctc cacgcccgcc tgctcaagct gctcaaaata gacgagctgc
      361 tcatccccga ggccatggcc gccgcctggc tcgcccgcac cctcaaggcc cccgacgtgc
      421 tcaacagctt ccaggtcggc cagggccacg agatcgtcga ggtcatcctc ccccccggca
      481 tggccggcag gaccctcgtc gagatggacc tgcgcggccg ttacgacctc aacctcgtca
      541 ccatcctgcg ccgcaaaaac atcaccaccc tcagctcctc cgccccgcag atcgagaaaa
      601 tcatcggcat ccccgagccc ggccgcgtct ttgaaaagga ggacatcctc ctcctcttcg
      661 gcaccgagaa aaacgtccgc cgcctcctcg ccgaccacgc cgcctgaccc gcgagccgcc
      721 cgccatcaca aggccgcgca cctccgccct gtgcgccgcc agcaccgccg ccagcaccgc
      781 cttcaggtcc cccgtgccat cgtggcaggc atgggccgcc cgcagcgggg cgagcgggaa
      841 gtcaggcagc ggagcgagca ggagcaggtg cgtcatgccc cggccaatgg ccggaaagcg
      901 cccgccgcaa atcgcaaaat caccggactc gccgccgcgc ggcttccact ccactggccg
      961 ttttgctgaa cgcgagtcat gccggggcgc gcggactcgc tcaattatag ctgatgtaag
     1021 tgatgacccg ggctttgtcg cgggtcagat agtggttcac ttgctggaga tgagtctggt
     1081 ctttgaagtg ggagtatcgc cagaccagcg gggcatccgg cagttggatg ggaatgccgg
     1141 ggacgcggcg aatgatgaag tccaagggat gaagatccac tcctgccgcg tcatggcttt
     1201 ccttgaattc gtggcgcttg agaagcagcg ggaactccgc gggatccagg ctgtagctca
     1261 tgatcagcgt ttgatggcca aagccacgct gcatccagac gccgaagtcg cccaggctgg
     1321 cggggcattc gtcggccaca tattcgcgaa tcagcccctc cggtgtgggc ggtccgggca
     1381 tggttgccag caataacatg ctaatcggca ggtggcccca aaagggcaga tattgccgca
     1441 tcggcagctc gcggcgcagc acgagttccg ccagccagcg aaggacaaaa ccgccggcca
     1501 gccagcacgg gtaggtgaac acgaagatca aactgccgaa gaagaccccg taggaaatgc
     1561 cattctccat cgggatgccg ccacttgcgg cagcgttgag cacgcacgcg ctttcatacg
     1621 ccaggatgga gagcacaatg cacagcgcca gtttcacagg ccgagcctat gccctgccgg
     1681 gcgggctgtg caagctccgt gtgacccggc caggcggcaa agccgggaca ggcgcgggcg
     1741 ggaagacagg gaatgccgcg cgcgggaaaa agtcccggca tggggggcgc ccgccgggcg
     1801 cttgttcgat gcctcgtcaa ggaagcgcca gccgccgccg gaacgggtga acccccgacg
     1861 cgcctccggc gccccccccc ccccccc
//
LOCUS       DAOMYJ010000473         1870 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Prosthecobacter sp. isolate AMR_MDS_5629
            NODE_24367_length_1870_cov_19.834711, whole genome shotgun
            sequence.
ACCESSION   DAOMYJ010000473 DAOMYJ010000000
VERSION     DAOMYJ010000473.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867850
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prosthecobacter sp. (wastewater metagenome)
  ORGANISM  Prosthecobacter sp.
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Verrucomicrobiaceae; Prosthecobacter.
REFERENCE   1  (bases 1 to 1870)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1870)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.30
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:34:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,742
            CDSs (total)                      :: 4,684
            Genes (coding)                    :: 4,676
            CDSs (with protein)               :: 4,676
            Genes (RNA)                       :: 58
            tRNAs                             :: 55
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1870
                     /organism="Prosthecobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24367_length_1870_cov_19.834711"
                     /isolate="AMR_MDS_5629"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1965333"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..541
                     /locus_tag="PLP58_23295"
     CDS             <1..541
                     /locus_tag="PLP58_23295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009961842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="xanthan lyase"
                     /protein_id="HRJ11524.1"
                     /translation="PGSPPAPPRPHPQALASIHAPGEVDRPEMVPFIVADPAALPGIV
                     VDETAAVLAGEWQYSTHTPPYVGLGYLHDMKSGKGAKSVTFTPELPVAGWYEVRLAHC
                     HNARRATAAPVTVRHACGETTVTVNQQQEPPHGRLFRSLGVFYFQAGRAGWVRVSNAG
                     TEPDKVVIADAVQFLESGR"
     gene            complement(572..1018)
                     /locus_tag="PLP58_23300"
     CDS             complement(572..1018)
                     /locus_tag="PLP58_23300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009965158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HRJ11525.1"
                     /translation="MAHPSPQPPDPAALPGLAKATMAAAKFPMLATVDGDQPRLRPVS
                     PVLVEGFVIYVANLRRYGKTAELAANPKAEFCYTDDGHNQVRITAAAEVLHDADLKRK
                     IWEANPLLRAYLQDISNPELIIYKFTPRRVRYMKEWALEYHEVPLD"
     gene            complement(1100..>1870)
                     /locus_tag="PLP58_23305"
     CDS             complement(1100..>1870)
                     /locus_tag="PLP58_23305"
                     /inference="COORDINATES: protein motif:HMM:NF024603.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HRJ11526.1"
                     /translation="VRAPAPRVSMRPAGVPLPANALRFYLHFTQPMEQGVFLDRLRLL
                     DDQEKEIPGPFRETELWSPDGARLTVWFHPGRQKTGVNLNLDEGPVMLPGRAYRLIVS
                     GSWRSAAGIPIGDDVSLRWTAGPEDHAPPLADHVEIHPPAASTTSPLRVTFSEPLDPA
                     MLATALQVRAVHDDSPVPGRAEAAPGGMAWTFHPHSPWAPGGYHLRILPELEDLAGNS
                     FAKPFEVDLQAAPSAPVAARPLAFAIRAAEKAASGSPE"
BASE COUNT          290 a          559 c          718 g          303 t
ORIGIN      
        1 gccgggctct ccgcccgcgc ctccgcgccc gcatccgcag gccctggcaa gcatccacgc
       61 gccgggcgag gtggaccggc cggagatggt gcctttcatc gtggccgacc ctgcggcgct
      121 gccgggcatc gtggtggacg agaccgccgc cgtgctggct ggagagtggc agtactccac
      181 ccacactccg ccttatgtgg ggctgggtta cctgcatgac atgaagtccg gcaaaggcgc
      241 gaagtcggtg actttcactc ccgagcttcc cgtggccggc tggtatgagg tgcggctggc
      301 gcattgccat aacgcgcgcc gcgccacggc cgctccagtc accgtgcgcc atgcctgcgg
      361 ggagaccacc gtgacggtga accagcagca ggagccgccg cacgggaggc tgttccgcag
      421 tctgggcgtg ttttattttc aggccggaag ggcgggctgg gtgcgtgtct cgaatgcggg
      481 caccgagccg gacaaggtgg tcatcgcgga tgccgtccag ttcctggaga gcgggcggtg
      541 agcgcgcctg cggggtgccg cgggccgccg gtcaatccaa aggcacctca tggtactcca
      601 gcgcccactc cttcatgtag cgcacgcgcc ggggggtgaa cttgtagatg atgagctcgg
      661 ggtttgaaat gtcctggaga taggcgcgca gcaggggatt ggcctcccag attttcctct
      721 tcaagtccgc gtcatgcaaa acctccgcgg cggcggtgat gcggacctgg ttgtggccgt
      781 cgtcggtgta gcaaaactcg gccttcgggt tggcggccag ctcggctgtc ttgccgtagc
      841 ggcgcagatt ggccacatag atgacgaagc cctcgacgag caccggggag accgggcgca
      901 gccggggctg gtcgccatcc acggtggcaa gcatggggaa cttcgcggct gccatggtgg
      961 ctttggccag gccaggcagg gcggcggggt cgggcggctg gggtgagggg tgtgccattg
     1021 ctgatgattg acggtccgcg cgcgcggcgg caacaggaat gttcgggccg gccgcctgcg
     1081 tttgccgccg ggcaaggaat cactcgggac tgccggacgc ggctttttcc gcggcgcgga
     1141 ttgcaaaggc aagcggacgc gccgcgactg gagcggacgg cgcggcctgg aggtccactt
     1201 cgaagggctt ggcgaagctg ttgccggcca ggtcttccag ctcaggcagg atgcgcagat
     1261 ggtagccgcc gggagcccac ggactgtgcg gatggaaggt ccatgccatg cctcccggcg
     1321 cggcctcggc gcggccgggg acgggactgt cgtcatgaac tgcccgcact tgcagggcgg
     1381 tggcgagcat ggcggggtcc agaggctcgc tgaaagtgac gcgcagaggc gaggtggtgg
     1441 aggcggctgg cgggtgaatt tccacatggt cagccaatgg cggggcatga tcctccggtc
     1501 cggcagtcca gcgcagggag acatcatccc cgatggggat gccggcggcg ctgcgccagg
     1561 agccggagac gatgaggcgg taggcgcgtc cgggaagcat gacagggcct tcgtcgaggt
     1621 tgagattgac gccggttttc tgccggccgg ggtggaacca gacggtgagg cgcgcgccgt
     1681 caggagacca cagctcggtc tcgcgaaagg ggcctgggat ttccttttcc tggtcgtcga
     1741 ggagccggag ccggtcgaga aaaaccccct gctccatggg ctgggtgaag tggaggtaaa
     1801 agcgcagggc attggccggc agcggcactc cggcggggcg catgctgacc cgcggcgcgg
     1861 gcgcgcggac
//
LOCUS       DAOMYJ010000474         1861 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Prosthecobacter sp. isolate AMR_MDS_5629
            NODE_24516_length_1861_cov_21.145072, whole genome shotgun
            sequence.
ACCESSION   DAOMYJ010000474 DAOMYJ010000000
VERSION     DAOMYJ010000474.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867850
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prosthecobacter sp. (wastewater metagenome)
  ORGANISM  Prosthecobacter sp.
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Verrucomicrobiaceae; Prosthecobacter.
REFERENCE   1  (bases 1 to 1861)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1861)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.30
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:34:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,742
            CDSs (total)                      :: 4,684
            Genes (coding)                    :: 4,676
            CDSs (with protein)               :: 4,676
            Genes (RNA)                       :: 58
            tRNAs                             :: 55
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1861
                     /organism="Prosthecobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24516_length_1861_cov_21.145072"
                     /isolate="AMR_MDS_5629"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1965333"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1021)
                     /gene="pilM"
                     /locus_tag="PLP58_23310"
     CDS             complement(<1..1021)
                     /gene="pilM"
                     /locus_tag="PLP58_23310"
                     /inference="COORDINATES: protein motif:HMM:TIGR01175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus assembly protein PilM"
                     /protein_id="HRJ11527.1"
                     /translation="MADSKSIAVLNLGSQRLSGAVFSRVGGDLVLKKYAIEEMSGDPT
                     VDASRLPQIRLGVQELASKLRLKGAAAHYAVAGHTVFSRFVKLPPVQKDRVAQIVEFE
                     AKQNVPFPINEVTWDYEIVSEGGGETEVILVAMKSETLNEINDQVAAAGMRTALVDLA
                     PLALFNAFRYSYPDVDEPCVIIDLGARSTNMVFVEDGKFFTRNVLVGGASITAAIAKE
                     FGIGFAEAERQKVMNGFVAQGGAVMEHEDPEIAALSKVIRNAATRLHGEVMRTINFYR
                     TQQGGAAPQRVFICGGGALLGNMALFLEEKLKLPVEILNPLRGVQLDRSVGADAAAAD
                     SAFMGE"
     gene            complement(1071..1694)
                     /locus_tag="PLP58_23315"
     CDS             complement(1071..1694)
                     /locus_tag="PLP58_23315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ11528.1"
                     /translation="MKRFLPALAAALIAPALMTHGQTAAPAVKVDAKKLKISAIETPQ
                     FQAGNVGEKRWRPKSWLEIDLEFDIRVPQSEGGRSGSLDSMTVNYYLAMNATTKDGKR
                     EVLKGSFNYVDIPAATTCHALAFASPATLRRVLQKDTFTAASDVQGWGYEILVGGQRV
                     AGDSSLGSSPWWEKTDSLNINDGALLMKQETPFSILWGDYDVSAKKQ"
BASE COUNT          297 a          636 c          577 g          351 t
ORIGIN      
        1 gctcgcccat gaaggcggag tcggcggcgg cggcgtccgc gccgacggag cggtcgagct
       61 gcacgccgcg cagcgggttg aggatctcga cgggcagctt gagcttctcc tccagaaaca
      121 gggccatgtt gcccagcagc gcgccgccgc cgcagatgaa cacacgctgg ggggccgcgc
      181 cgccctgctg ggtgcggtag aagttgatgg tgcgcatcac ctccccgtgc aggcgggtgg
      241 cggcgttgcg gatgaccttg gagagcgcgg cgatctccgg gtcctcatgc tccatgaccg
      301 cccccccctg ggcgacgaat ccgttcatca ccttctgccg ttcagcctcg gcaaagccaa
      361 tgccgaactc cttggcgatg gccgcggtga tggaggctcc gccgaccagg acgttgcggg
      421 tgaaaaactt cccatcctcg acgaacacca tgttcgtgga gcgcgcgccc aggtctatga
      481 tcacgcacgg ctcgtccacg tcagggtagc tgtagcggaa cgcgttgaac aacgccagcg
      541 gcgccaggtc caccagcgcg gtcctcatcc cggccgcggc cacctggtcg ttgatctcgt
      601 tgagggtctc cgacttcatc gccaccagga tgacctccgt ctcgccgccg ccttcggaga
      661 cgatctcgta atcccaggtg acctcgttga tcgggaacgg cacgttttgc ttggcctcga
      721 actcgacgat ctgcgccacg cggtccttct gcaccggggg cagcttgaca aaacgcgaga
      781 acaccgtgtg ccccgccacc gcgtagtgcg ccgccgcccc cttgagccgc agcttcgacg
      841 ccagctcctg gacgccgagc cggatctggg gaagccgcga ggcatccacc gtcgggtccc
      901 cggacatttc ctcaatggcg tattttttga ggaccaaatc gccgccgact ctgctgaaca
      961 ccgccccgga caacctttgg gagccgagat tcaggactgc tatgcttttg ctgtctgcca
     1021 tgtggattgt tgaatgaacc tgccattgcg ccgcccgtca agagccgcgg ttactgcttc
     1081 ttggcggaga catcgtagtc gccccagaga atggaaaacg gcgtctcctg cttcatgagc
     1141 agcgccccgt cgttgatgtt gagcgagtcc gtcttctccc accacgggct gctgcccagg
     1201 ctggagtcac cggccacgcg ctggccgccg acgaggatct cgtagcccca gccctgcacg
     1261 tccgaggccg cggtgaaggt gtccttctgc agcacgcgcc tgagcgtggc gggcgacgcg
     1321 aaggccagcg cgtggcaggt cgtggccgcg gggatgtcca catagttgaa cgagcctttg
     1381 agcacctccc gcttgccgtc cttcgtcgtc gcgttcatcg ccagataata gttcaccgtc
     1441 atcgagtcca ggctgccgct gcgcccgccc tcggactgcg gcacccggat gtcgaactcc
     1501 aggtcaatct ccagccacga cttcggacgc cagcgcttct cccccacgtt gccggcctgg
     1561 aactgcggcg tctcgatcgc cgagattttc agcttcttgg cgtccacttt caccgcgggg
     1621 gccgccgtct ggccgtgcgt catcagggcc ggcgcgatca gcgccgccgc cagggctggg
     1681 agaaatcgtt tcatgtgcat ggaaggaggt tgagggttgg cccgtgtccg ccgccgtttc
     1741 atccggcggc tgacaggatt atttttcgtt ttatccgcaa agccgcgcgc ccggcaagat
     1801 gtgatttcaa aaaagtggaa attcttttcc gccgccgccc cgccgcgccc cgccgcgctc
     1861 c
//
LOCUS       DAOMYJ010000475         1857 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Prosthecobacter sp. isolate AMR_MDS_5629
            NODE_24596_length_1857_cov_14.194784, whole genome shotgun
            sequence.
ACCESSION   DAOMYJ010000475 DAOMYJ010000000
VERSION     DAOMYJ010000475.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867850
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prosthecobacter sp. (wastewater metagenome)
  ORGANISM  Prosthecobacter sp.
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Verrucomicrobiaceae; Prosthecobacter.
REFERENCE   1  (bases 1 to 1857)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1857)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.30
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:34:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,742
            CDSs (total)                      :: 4,684
            Genes (coding)                    :: 4,676
            CDSs (with protein)               :: 4,676
            Genes (RNA)                       :: 58
            tRNAs                             :: 55
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1857
                     /organism="Prosthecobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24596_length_1857_cov_14.194784"
                     /isolate="AMR_MDS_5629"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1965333"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1857
                     /locus_tag="PLP58_23320"
     CDS             <1..>1857
                     /locus_tag="PLP58_23320"
                     /inference="COORDINATES: protein motif:HMM:NF019223.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF1549 and DUF1553 domain-containing protein"
                     /protein_id="HRJ11529.1"
                     /translation="VASAVKPRPVDIEQGRRHWAFQPVADVPTPKGGHPIDVFLSAKL
                     AEKGLDFAAPADAHTFLRRATYDLTGLPPTPEDTAAFTAAHASDPDRATRQLIDRLLA
                     SPHYGEKWGRHWLDVARYADSNGLDENIALGTAWRYRDYVVRAFNEDKPFDRFLTEQL
                     AGDLLPARTLAERHEHATATAFLNLGAKVLAEPDKEKLAMDVIDEQLETFGRAFLGMT
                     LGCVRCHDHKFDPVTQADYYALAAIFKSTTSFSSDRMGALSLAHETPLGDFEDFAAVK
                     SAEQALAAKKKEVAAAQAEAKKKPGPEAEAKVMRLMDEEEAIEKSLPDLPTVISVRDA
                     ERLTPQLAIHIRGSHLTLGKKVDRGFIQVMQAAHSPQPALPGSQSGRLELARWLTSPQ
                     HPVTARVIANRVWTWHFGRGIAGTPDNFGLLGEAPTHPDLLDWLARWLPANGWSLKDM
                     HRLIMTSRAYRQGGKARARGARLDEADPENRLLSHFPMRRLEAEEIRDSLLAVAGLLD
                     LSMGGKTVPLRNRQFVFNHTSKDATKYDSTRRALYLPVIRNNLYDLFQQFDYPDPAVS
                     TGQRNSTIVSPQALLLMNSPLAEQAARGFASRLKTGASLEEKVRRAMQLAYA"
BASE COUNT          333 a          792 c          491 g          241 t
ORIGIN      
        1 cggtggcctc cgccgtgaag ccgcgcccgg tggacatcga gcagggccgc cgccattggg
       61 ccttccagcc cgtggccgac gtgccgacgc cgaagggcgg ccaccccatt gatgtcttcc
      121 tttccgcaaa gctcgctgaa aaaggcctcg acttcgccgc gcccgcggac gcccacacct
      181 tcctgcgccg cgccacctac gacctcaccg gcctgccccc cacgcccgaa gataccgccg
      241 ccttcaccgc cgcccacgcc agcgatcccg accgcgccac ccgccagctc attgaccgcc
      301 tgctcgcctc cccgcactac ggggaaaaat ggggccgcca ctggctggac gtggcccgct
      361 acgccgactc gaacggcctg gatgaaaaca tcgccctcgg caccgcctgg cgttaccgcg
      421 actacgtcgt gcgcgccttc aacgaggaca agcccttcga ccgcttcctc accgagcagc
      481 tcgccggcga cctcctgccc gcgcgcaccc tggccgagcg ccacgagcac gccactgcca
      541 ccgccttcct gaatctcggc gccaaagtcc tcgccgagcc ggacaaggaa aagctggcca
      601 tggatgtcat agacgagcag ctcgagacct tcgggcgcgc cttcctcggc atgaccctcg
      661 gctgcgtgcg ctgccacgac cacaaattcg accccgtcac ccaggccgac tactacgccc
      721 tggccgccat cttcaagagc accacctcct tcagctccga ccgcatgggc gcgctctccc
      781 tcgcgcacga gaccccgctc ggagacttcg aggacttcgc cgccgtgaag tccgccgagc
      841 aggccctggc cgccaagaaa aaagaagtcg ccgccgccca ggccgaggcc aaaaagaagc
      901 ccggccccga agccgaggcc aaagtcatgc gcctcatgga cgaggaggag gccattgaaa
      961 aatccctgcc cgacctgccc accgtcatca gcgtgcgcga cgccgaaaga ctcacgccac
     1021 agctcgccat ccacatccgc ggcagccacc tcaccctcgg aaaaaaagtg gaccgcggct
     1081 tcatccaggt catgcaggcc gcgcactcgc cgcagcccgc cctgcccggc tcccagagcg
     1141 gccgcctgga gctcgcgcgc tggctcacct ccccgcagca ccccgtcacc gcccgcgtca
     1201 tcgccaaccg cgtctggacc tggcacttcg gccgcggcat cgccggcacg ccggacaatt
     1261 tcggcctgct cggcgaggcc cccacccacc ccgatctgct cgactggctg gccagatggc
     1321 tgcccgccaa cggctggagc ctcaaggaca tgcaccgcct catcatgacc agccgcgcct
     1381 accgccaggg cggcaaggcc cgggcccgcg gcgctcgtct ggacgaggcc gaccccgaga
     1441 accgcctcct ctcccacttc cccatgcgcc gcctcgaggc cgaggaaatc cgcgacagcc
     1501 tcctggccgt ggccggcctg ctggacctca gcatgggcgg caaaaccgtc cccctgcgca
     1561 accgccagtt cgtcttcaac cacacctcca aagacgccac caaatacgac agcacccgcc
     1621 gcgccctcta cctgcccgtc atccgcaaca acctctacga cctcttccag cagttcgact
     1681 accccgaccc cgccgtctcc accggccagc gcaacagcac catcgtctcc ccccaggccc
     1741 tcctgctcat gaacagcccc ctcgcagagc aggccgcccg cggtttcgcc tcccgcctga
     1801 aaaccggcgc gtccttggag gaaaaagtcc gccgcgccat gcagctcgcc tacgccc
//
LOCUS       DAOMYJ010000476         1856 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Prosthecobacter sp. isolate AMR_MDS_5629
            NODE_24618_length_1856_cov_18.255414, whole genome shotgun
            sequence.
ACCESSION   DAOMYJ010000476 DAOMYJ010000000
VERSION     DAOMYJ010000476.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867850
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prosthecobacter sp. (wastewater metagenome)
  ORGANISM  Prosthecobacter sp.
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Verrucomicrobiaceae; Prosthecobacter.
REFERENCE   1  (bases 1 to 1856)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1856)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.30
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:34:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,742
            CDSs (total)                      :: 4,684
            Genes (coding)                    :: 4,676
            CDSs (with protein)               :: 4,676
            Genes (RNA)                       :: 58
            tRNAs                             :: 55
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1856
                     /organism="Prosthecobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24618_length_1856_cov_18.255414"
                     /isolate="AMR_MDS_5629"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1965333"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..286
                     /locus_tag="PLP58_23325"
     CDS             <1..286
                     /locus_tag="PLP58_23325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ11530.1"
                     /translation="RMPRRAPAVPERALAPVYRQVYVFVLQEKEGKKFVERRAIETGL
                     RKTGFVEAASGLKAGETVVTDGYMGLRDGGEVEVSGRFKQAAPAYNPRNN"
     gene            308..>1856
                     /locus_tag="PLP58_23330"
     CDS             308..>1856
                     /locus_tag="PLP58_23330"
                     /inference="COORDINATES: protein motif:HMM:NF013069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HRJ11531.1"
                     /translation="MFLSDVSIRRPVLATVANLLLMAFGLVAYTRMQVREYPDIEPPI
                     ISIETTYPGAAAAVVERRVTQLIEDRISGVEGIKNVDSISSDGLSQITVEFSLDRGID
                     NAASDVREAVSSILDELPDEVRPPEITKQDAVTEVLMWLNLTHPDMQPVELADYAERY
                     LVDRFSQLPGVARIRISGATRFSMRIWLDREALAARGLTVVDVEEALRRQNVELPAGA
                     VQARDRTITVRIRREFTTVEEFQSLVLKRGEDGYQVRLGEVARVELGAEDARRRFHGN
                     GVPMAALGIIKQSRANTLDVARAAKAEAARLAPSLPEGMRLEQSYDTSQFIEASLVEV
                     FKTLGIAVALVVLVIYLFLGTARATLVPAVAVPVSIIATCIILYFIGFSVNVLTLLAV
                     VLGIGLVVDDAIVVLENIHRRIEGGEAPLAAAYMGARQVGFAVVATTLVLVAVFVPIS
                     FLEGDAGRLFAEFSVTLAVAVSFSSFVALTLCPMIASRLLRPAGRANLLTGAVDAVFR
                     ALQKGYAA"
BASE COUNT          260 a          621 c          682 g          293 t
ORIGIN      
        1 ccgcatgccg cggcgcgcgc ccgccgtgcc ggagcgcgcg ctggcgccgg tttaccgcca
       61 ggtgtacgtc tttgtgctcc aggagaagga ggggaagaaa ttcgtcgagc gccgcgccat
      121 cgagaccggc ctgcgcaaga ccggctttgt ggaggcggcc tccggcctga aagccgggga
      181 gacggtggtg acggacggct acatgggcct gagggacggc ggcgaggtgg aggtcagcgg
      241 ccgcttcaag caggccgcgc ccgcctacaa cccgcgcaac aactgagccg cgagcacgcc
      301 cagcgccatg tttctctccg atgtctccat ccgccgcccc gtgctggcca ccgtggcgaa
      361 cctgctgctc atggccttcg gcctggtggc ctacacgcgc atgcaggtgc gcgagtatcc
      421 cgacatcgag ccgcccatca tctccatcga gacgacgtat ccgggcgcgg cggcggcggt
      481 ggtggagcgg cgcgtgacgc agctcatcga ggaccgcatc agcggcgtgg agggcatcaa
      541 gaatgtggac tccatcagca gcgacgggct ttcccagatc acggtggagt tctccctgga
      601 ccgtggcatt gacaacgccg ccagcgacgt gcgcgaggcc gtctccagca tcctcgacga
      661 gctgcccgac gaggtgcgcc cgccggagat caccaagcag gacgcggtga cggaggtgct
      721 catgtggctg aacctgaccc acccggacat gcagccggtg gagctggccg actacgcgga
      781 gcgctacctg gtggaccgct tctcccagct ccccggcgtg gcgcgcatcc gcatcagcgg
      841 cgccacgcgc ttctccatgc gcatctggct ggaccgcgag gcgctggccg cgcgcgggct
      901 gacggtggtg gacgtggagg aggcgctgcg gcggcagaat gtggagctgc ccgccggggc
      961 cgtgcaggcg cgggaccgga cgatcacggt gcgcatccgg cgggagttca ccacggtgga
     1021 ggagttccag agcctggtgc tcaagcgcgg ggaggacggc taccaggtgc gcctgggcga
     1081 ggtggcgcgc gtggagctgg gcgcggagga cgcgcgccgc cgcttccacg gcaacggcgt
     1141 gccgatggcc gcgctgggca tcatcaagca gtcccgcgcc aacacgctgg acgtggcgcg
     1201 cgcggcgaag gcggaggccg cccgcctcgc gccctcgctg ccggagggca tgaggctgga
     1261 gcagagctat gacacgtcgc agttcatcga ggcctcgctg gtggaggtgt tcaagacgct
     1321 gggcatcgcc gtggcgctgg tggtgctggt catttatctt tttctgggca ccgcgcgcgc
     1381 cacgctggtg cccgcggtgg cggtgccggt gtccatcatc gccacctgca tcatcctgta
     1441 cttcatcggc ttctcggtca atgtgctgac gctgctggcc gtggtgctgg gcatcggcct
     1501 ggtggtggat gacgccatcg tcgtgctgga gaacatccac cgccgcatcg agggcgggga
     1561 ggcgccgctg gccgccgcct acatgggcgc gcggcaggtg ggcttcgccg tggtggccac
     1621 cacgctggtg ctggtggcgg tgttcgtgcc catcagcttc ctggaggggg acgcgggcag
     1681 gctcttcgcg gagttctccg tgaccctggc cgtggcggtg agcttttcca gcttcgtggc
     1741 gctgaccctg tgcccgatga tcgcctccag gctgctgcgc cccgccggcc gcgccaacct
     1801 gctgaccggc gccgtggacg ccgtcttccg cgccctccag aaaggctacg ccgccc
//
LOCUS       DAOMYJ010000477         1853 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Prosthecobacter sp. isolate AMR_MDS_5629
            NODE_24669_length_1853_cov_20.582870, whole genome shotgun
            sequence.
ACCESSION   DAOMYJ010000477 DAOMYJ010000000
VERSION     DAOMYJ010000477.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867850
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prosthecobacter sp. (wastewater metagenome)
  ORGANISM  Prosthecobacter sp.
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Verrucomicrobiaceae; Prosthecobacter.
REFERENCE   1  (bases 1 to 1853)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1853)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.30
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:34:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,742
            CDSs (total)                      :: 4,684
            Genes (coding)                    :: 4,676
            CDSs (with protein)               :: 4,676
            Genes (RNA)                       :: 58
            tRNAs                             :: 55
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1853
                     /organism="Prosthecobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24669_length_1853_cov_20.582870"
                     /isolate="AMR_MDS_5629"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1965333"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(3..305)
                     /locus_tag="PLP58_23335"
     CDS             complement(3..305)
                     /locus_tag="PLP58_23335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009964293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ11532.1"
                     /translation="MPKENDMDETARLRQDAWVGDAVLELYARSWVLRASGAVDAEMK
                     TRLTCNQFLNCAGNPTQVEAGIGVIYQRQGLDAAFAWIRENLEPLFLKQESNRARR"
     gene            476..982
                     /locus_tag="PLP58_23340"
     CDS             476..982
                     /locus_tag="PLP58_23340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ11533.1"
                     /translation="MKAQQLLQAINPELRQQIIAYVQTDERPAYRALIQNLAQQRRLR
                     PQFVLEKSRPKQAEWLLAQLYLKTNDPVAEQLLQIWLLKARRPMLVTFLDAVGITHDD
                     KGQVEDLPDEIPEDNAAAGIQALLAAHPAAEAALYLHMFQLQRPGGWDGLAKALAACT
                     EVQLPSAP"
     gene            979..1785
                     /locus_tag="PLP58_23345"
     CDS             979..1785
                     /locus_tag="PLP58_23345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009958202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase/citrate lyase family protein"
                     /protein_id="HRJ11534.1"
                     /translation="MKLRPSRVLREVRAGRHATVIKLNLGDPRIVELAGLAGASAVWL
                     CNEHVPNDWTNLEHQVRAAKLHDMDTIVRVSKGSYSDYVKPFELDATGIMVPHVTSAA
                     EARAIVDMVRCHPLGRRPMDGGNADGAFCQIPLADYARHCNEERFVILQIESPEGLEA
                     VEEIAAVPGFDMLLFGAGDYSHRIGILGQATAPEVVAARKRVAAAALKHGKHVAVASL
                     FGQKDEIIAEGTSVFTLGADVIALGDAFHHMVRAFHPPAPQAADAGSIYR"
BASE COUNT          313 a          734 c          528 g          278 t
ORIGIN      
        1 ggtcagcggc gggcgcggtt tgactcctgc ttcaaaaaca gcggctccag attctcccgg
       61 atccaggcaa aggccgcgtc gagtccctgg cgctggtaaa tgacgccgat gcccgcctcc
      121 acctgcgtgg ggttgcccgc gcagttgagg aattgattgc aggtcaggcg ggtcttcatc
      181 tccgcgtcca ccgcgccgct cgcgcgcagc acccaggagc gggcgtaaag ctccagcacg
      241 gcatccccca cccaggcgtc ctggcgcagc cgtgccgtct catccatgtc gttttctttg
      301 ggcatcttcc ggcagtgtgc gcgggcgcgg gcaaaatgcg cggcaatttt ttgccccgcg
      361 ttttcctttc ccgctcacgc gcgggccgtg tgttggcggt tgcggctgcg gatggcccat
      421 gttagcggaa ccgcccgaac ccaaccccac cctccacgcc caccgaccgg cgaccatgaa
      481 agcccagcaa ctcctccaag ccatcaaccc cgagctccgc cagcagatca tcgcctacgt
      541 ccagactgac gagcgccccg cctaccgcgc cctgatccag aacctggccc agcagcgccg
      601 cctccggccc cagttcgtcc tggaaaaatc ccgccccaag caggccgagt ggctgctcgc
      661 ccagctctat ttaaaaacca atgaccccgt ggccgagcag ctcctgcaga tctggctgct
      721 caaagcccgg cgccccatgc tcgtcacctt cctcgacgcc gtcggcatca cccacgatga
      781 caaaggccag gtcgaggacc tgcccgacga aatccccgag gacaatgccg ccgccggcat
      841 ccaggccctg ctcgccgccc accccgccgc cgaggccgcg ctctacctcc acatgttcca
      901 gctccagcgt ccgggaggct gggacggcct ggccaaagcc ctcgccgcct gcaccgaagt
      961 ccagctcccc tccgcgccat gaagctccgc cccagccgcg tcctgcgcga ggtccgcgcc
     1021 gggcgccacg ccaccgtcat caagctcaac cttggcgacc cccgcatcgt cgagctcgcc
     1081 ggcctcgccg gagccagcgc cgtctggctg tgcaacgagc atgtgcccaa cgactggacc
     1141 aacctcgagc accaggtccg cgccgccaag ctgcacgaca tggacaccat cgtccgcgtc
     1201 agcaaaggct cctacagcga ctatgtgaag cccttcgagc tcgacgccac cggcatcatg
     1261 gtcccccacg tcaccagcgc cgcggaggcc cgcgccatcg tggacatggt ccgctgccac
     1321 ccgctcggcc gccgccccat ggacggcggc aatgccgacg gcgccttctg ccagatcccc
     1381 ctggccgact acgcgcgcca ctgcaacgag gagcgcttcg tcatcctcca gatcgagtcc
     1441 cccgagggcc ttgaggccgt cgaggaaatc gccgccgtcc ccggcttcga catgctgctc
     1501 tttggcgcgg gagactacag ccaccgcatc ggcatcctcg gccaggccac cgcgccggag
     1561 gtcgtcgccg cgcgcaaacg cgtcgccgcc gccgccttga aacatggcaa gcatgtcgcc
     1621 gtcgcctccc tcttcgggca aaaggacgag atcatcgccg aaggcacctc cgtcttcacc
     1681 ctcggcgccg acgtcatcgc cctcggcgac gccttccacc acatggttcg cgccttccac
     1741 ccccccgccc cccaggccgc cgacgccggc tccatctacc gctgaggcgg agtggggaat
     1801 gcggaatgcg gaatgcggaa tgcggaatgc ggaatgcgga atgcggaatg cgg
//
LOCUS       DAOMYJ010000478         1829 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Prosthecobacter sp. isolate AMR_MDS_5629
            NODE_25142_length_1829_cov_20.348929, whole genome shotgun
            sequence.
ACCESSION   DAOMYJ010000478 DAOMYJ010000000
VERSION     DAOMYJ010000478.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867850
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prosthecobacter sp. (wastewater metagenome)
  ORGANISM  Prosthecobacter sp.
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Verrucomicrobiaceae; Prosthecobacter.
REFERENCE   1  (bases 1 to 1829)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1829)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.30
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:34:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,742
            CDSs (total)                      :: 4,684
            Genes (coding)                    :: 4,676
            CDSs (with protein)               :: 4,676
            Genes (RNA)                       :: 58
            tRNAs                             :: 55
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1829
                     /organism="Prosthecobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25142_length_1829_cov_20.348929"
                     /isolate="AMR_MDS_5629"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1965333"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..822)
                     /locus_tag="PLP58_23350"
     CDS             complement(<1..822)
                     /locus_tag="PLP58_23350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009959984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HRJ11535.1"
                     /translation="MTDSYSTNQNTEAACQDVERLKQALNEVLFGQKQLVELVLCGVI
                     ARGHVLLEGLPGLGKTELVKGLSKALGLTTRRVQFTPDLLPGDITGNPVLQEVDGRRE
                     FVFQPGPVFTNLLLADEINRASPKTQSALLEAMQERRVTVLGQTHTLPDPFFVLATQN
                     PIELEGTYPLPEAQVDRFLFKLEVLRNPAETLAKIVLHRELGVEPQVDAVLTPEAFTR
                     LQTEARRIFLPEVVADYIARLVDATHPGQSQAARGVRYGASPMHSPALPYPRARWT"
     gene            complement(876..>1829)
                     /locus_tag="PLP58_23355"
     CDS             complement(876..>1829)
                     /locus_tag="PLP58_23355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ11536.1"
                     /translation="WRWQMIAAPLALFAGGPALALWLPLTSEAAAAPAVQPQAWAKME
                     DWLEKLREEQILSEEEKQEQQAKIERLRGQEPEKWFSHESLHAGDTLKEQLQREIQRL
                     GQNLAGAEMSLNILQNHADQLSDAARERLLNDFQQALAGLQSGGLELDPAFLEALASV
                     DPKNLKSLSEDQLKQLRESMKNKAGACEGLCENPGFLGDGEGEDDALAAMLGLLKQEG
                     EGDEPGRGGISRGPGTAPLTLADEENNFGTSNPEAVSNEDLSRARVGDLTALRDGKHE
                     VEKNYQPGQSAGSATTGQGGSQVWRETLTPAEKAVLKRVFR"
BASE COUNT          286 a          598 c          586 g          359 t
ORIGIN      
        1 ggtccaacgg gcgcgcgggt agggcagggc gggcgagtgc atggggctgg ctccataacg
       61 caccccgcgc gccgcctggg actggccggg atgcgtggca tccaccagcc gggcgatgta
      121 gtccgccacg acttccggca gaaaaatgcg ccgcgcctcg gtctgaagcc gcgtgaaggc
      181 ctccggcgtc agcacggcgt ccacctgcgg ctccacgccc agctcgcggt gcaggacgat
      241 ctttgccaag gtctccgccg ggttgcgcag cacctccagc ttgaacaaaa agcggtccac
      301 ctgcgcctcg ggcagcggat aggtgccttc gagttcgatg gggttctgcg tggccagcac
      361 gaaaaaggga tcgggcagcg tgtgcgtctg ccccaacacg gtgacgcgcc tttcctgcat
      421 ggcttccagc agggcggact gggtcttggg cgaggcacgg ttgatctcat ccgccagcag
      481 cagattcgtg aataccggcc ccggctggaa aacgaactcc cgccggccat ccacctcctg
      541 gagcacgggg tttcccgtga tgtcgcccgg cagcaggtcg ggggtgaact gcacgcgccg
      601 cgtggtgagg ccgagagcct tcgacaagcc cttcaccagt tccgtcttgc ccaggccggg
      661 cagcccctcc agcagtacat gcccgcgcgc gatgacgccg cagagcacga gttcgacgag
      721 ctgtttctgg ccaaagagca cttcattaag cgcctgcttc aggcgctcca catcctggca
      781 ggccgcctcc gtgttctgat tggtggagta agagtccgtc attttgccga ctcaagcaga
      841 tgccatgcct gcaagtcgag atgaaaatcg catcctcagc ggaagacgcg cttgagcacc
      901 gctttttccg cgggggtcaa agtctcgcgc cagacttgag agccgccctg gccggtggtg
      961 gctgatcccg cgctttgtcc cggctgatag ttcttttcca cttcatgttt gccgtcgcgc
     1021 agcgcggtga ggtcgccgac tcgggcgcgg gagaggtcct cgttggagac agcctcggga
     1081 ttgctggtgc cgaagttgtt ttcctcatca gcgagagtca gcggcgcggt gccggggccc
     1141 cggctgatgc cgccccggcc gggttcatcg ccttcgcctt cctgcttgag caggccgagc
     1201 atggcggcca gggcatcgtc ttcgccctcg ccatcaccga gaaagccggg gttttcacag
     1261 agaccctcgc aggcgcctgc cttgttcttc atggattcgc ggagctgctt caactgatct
     1321 tcgctcaagc ttttgaggtt tttcggatcc acggaggcga gcgcctccaa aaacgcgggg
     1381 tccagctcca ggccgccgga ttgaagcccg gccagcgctt gctgaaaatc gttcagcagg
     1441 cgctcgcgcg cggcatcgct gagctgatcg gcgtggtttt gcaggatgtt caggctcatt
     1501 tcagctccgg cgaggttttg cccaagacgc tggatttctc tttggagctg ttctttgaga
     1561 gtgtcgcccg cgtgcaggct ctcgtggctg aaccacttct ccggctcctg cccgcgcagg
     1621 cgctcgatct tggcctgctg ctcctgcttt tcctcctcgc tgaggatttg ctcctcgcgc
     1681 agcttttcaa gccagtcctc cattttggcc catgcctgcg gctgcactgc gggtgcggcc
     1741 gccgcctcgg aagtcagcgg cagccacaac gccagagcgg gaccgcccgc gaacagggcc
     1801 aggggcgcgg cgatcatctg ccagcgcca
//
LOCUS       DAOMYJ010000479         1825 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Prosthecobacter sp. isolate AMR_MDS_5629
            NODE_25218_length_1825_cov_21.003955, whole genome shotgun
            sequence.
ACCESSION   DAOMYJ010000479 DAOMYJ010000000
VERSION     DAOMYJ010000479.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867850
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prosthecobacter sp. (wastewater metagenome)
  ORGANISM  Prosthecobacter sp.
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Verrucomicrobiaceae; Prosthecobacter.
REFERENCE   1  (bases 1 to 1825)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1825)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.30
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:34:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,742
            CDSs (total)                      :: 4,684
            Genes (coding)                    :: 4,676
            CDSs (with protein)               :: 4,676
            Genes (RNA)                       :: 58
            tRNAs                             :: 55
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1825
                     /organism="Prosthecobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25218_length_1825_cov_21.003955"
                     /isolate="AMR_MDS_5629"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1965333"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..404)
                     /locus_tag="PLP58_23360"
     CDS             complement(<1..404)
                     /locus_tag="PLP58_23360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ11537.1"
                     /translation="MMPRHSREPAADGPVRWSPFDDWESPWCLEDCSAGDVFARPAPS
                     EKASHGHKTRAPKGRVQAARPLTGEVLEFEAADEFLTPAPPSSPPQRPFPAREPVVKK
                     KPVVVTEKAAPEPDPAPAPEFPQAPQTRKTKE"
     gene            complement(418..915)
                     /locus_tag="PLP58_23365"
     CDS             complement(418..915)
                     /locus_tag="PLP58_23365"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009961756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA pyrophosphohydrolase"
                     /protein_id="HRJ11538.1"
                     /translation="MSQLPALHPMPVVYRPNVAAILTNAQRQIFIAERVNIRGAWQFP
                     QGGIDDGEDAETAVFRELAEEIGVRREMLELLDRRGGYRYAFPKPRLKYGIYGGQEQT
                     YFLMRFLGRDTDVNLAATHQEFARWRWIHPEEFQLSWTPMFKRGVYRQVFLDFFGLAL
                     ENPPQ"
     gene            1051..>1825
                     /locus_tag="PLP58_23370"
     CDS             1051..>1825
                     /locus_tag="PLP58_23370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ11539.1"
                     /translation="MRRTTLVLLACLSIHAARAAEAVKAPLMAGAASSNITPELGDKI
                     VGGFLPFPSTHVNDELHARCLVLDDGKTRLALVVCDLLGLHRSLSLEARRLIESETGI
                     PPENVLISATHTHSGTNAHSTDPRTFVSDMEISPYQRFVARRIADGVRRARNLLRPAE
                     IAFGRVDAPEHVFIRRWFMKEGTAPTNPFGKIDQVKMNPPGGSPDLVEPAGVTDPAVS
                     FFVLREPGGSLIALYSAYSLHYVGGVGSGHISADYYGMYC"
BASE COUNT          310 a          649 c          519 g          347 t
ORIGIN      
        1 tcctccttcg tcttcctggt ctgcggtgcc tgcggaaatt ccggcgcagg cgcggggtcc
       61 ggctcgggcg ctgctttttc cgtcacgacg acgggcttct ttttcaccac cggctcacgg
      121 gccggaaaag gacgctgcgg tggtgaagat ggtggagctg gcgtcagaaa ctcgtccgcg
      181 gcttcgaact ccagcacctc gcccgtcagg ggccgggcgg cctgcacccg acccttcggc
      241 gctctggtct tgtgcccatg agacgctttt tcagacggcg cgggacgcgc aaagacatcc
      301 cccgccgagc aatcctccag gcaccaggga gactcccagt catcgaaggg cgaccagcgc
      361 acgggaccgt ccgcggcggg ttctcggctg tggcggggca tcatgagatg agggctttca
      421 ctgcggcgga ttctccagcg cgagcccgaa aaagtcgagg aagacctggc gatagacccc
      481 gcgcttgaac atcggcgtcc aggaaagctg gaactcctcc ggatgaatcc agcgccagcg
      541 cgcgaactcc tgatgcgtcg cggccaggtt cacatccgtg tccctgccca ggaagcgcat
      601 caaaaagtag gtctgttcct ggccgccata gatgccgtat ttcaggcgcg gcttgggaaa
      661 agcataccgg tagccgccgc gccggtccag cagctcaagc atctcgcgcc tcacgccgat
      721 ctcctccgcc agctctcgaa agaccgccgt ctccgcgtcc tcgccatcgt caatgccgcc
      781 ctgcgggaac tgccacgcgc cgcgtatgtt cacccgttca gcgatgaaga tttgtctttg
      841 cgcgttggtc aggatggcgg cgacattcgg ccggtaaacg acaggcatgg gatgcagtgc
      901 gggaagctgt gacatgttcg ctgtcatgat aatacaggtt tgtgaaattt ccgtcgtcat
      961 ttcaggactt tttcaccatc tccgatggcg ggacgcattt ttcaggcctg tcccaggaaa
     1021 agccgtgcct ccaatgccgg tattgtctcc atgcgccgaa ccacccttgt cctgctcgcc
     1081 tgcctctcca tccatgccgc gcgcgcggcg gaagccgtca aagccccgct catggccggg
     1141 gccgcctcca gcaacatcac ccccgagctg ggtgacaaga ttgtcggcgg ttttctccct
     1201 ttcccctcca cccacgtcaa cgacgagttg cacgcccgat gcctcgtgct ggatgacggc
     1261 aaaaccaggc tcgcgctcgt cgtctgcgat ttgctcggac tgcaccgcag cctttccctg
     1321 gaggcgcgca ggctcattga gtccgagacc ggcatcccgc ctgaaaacgt gctcatttcc
     1381 gccactcaca cccactccgg caccaacgcc cacagcaccg acccgcgcac cttcgtctcc
     1441 gacatggaga tcagtcccta ccagcgcttc gtcgcccgcc gcatcgccga tggcgttcgc
     1501 cgtgcgcgca acctgctccg ccccgccgag atcgcctttg gccgggtgga cgccccggag
     1561 cacgtcttca tccgccgctg gttcatgaag gagggcaccg ccccgaccaa ccccttcggc
     1621 aagattgacc aggtgaaaat gaatcccccc ggaggcagcc ccgacctcgt cgaacccgcc
     1681 ggcgtcaccg accccgcagt ctcctttttc gtcctgcgcg agccgggcgg cagcctcatc
     1741 gccctctact ccgcctactc cctgcactat gtcggcggcg tcggctccgg ccacatctcc
     1801 gccgattatt acggcatgta ttgcg
//
LOCUS       DAOMYJ010000480         1824 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Prosthecobacter sp. isolate AMR_MDS_5629
            NODE_25244_length_1824_cov_18.947993, whole genome shotgun
            sequence.
ACCESSION   DAOMYJ010000480 DAOMYJ010000000
VERSION     DAOMYJ010000480.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867850
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prosthecobacter sp. (wastewater metagenome)
  ORGANISM  Prosthecobacter sp.
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Verrucomicrobiaceae; Prosthecobacter.
REFERENCE   1  (bases 1 to 1824)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1824)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.30
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:34:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,742
            CDSs (total)                      :: 4,684
            Genes (coding)                    :: 4,676
            CDSs (with protein)               :: 4,676
            Genes (RNA)                       :: 58
            tRNAs                             :: 55
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1824
                     /organism="Prosthecobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25244_length_1824_cov_18.947993"
                     /isolate="AMR_MDS_5629"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1965333"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(224..547)
                     /locus_tag="PLP58_23375"
     CDS             complement(224..547)
                     /locus_tag="PLP58_23375"
                     /inference="COORDINATES: protein motif:HMM:NF025597.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GYF domain-containing protein"
                     /protein_id="HRJ11540.1"
                     /translation="MNWYYDNQGEAAGPLDLPGLRAERDAGRIRADTLVWCPGAQEWQ
                     AVGLLQPAWWTQAEEKEPLQPASPAGDTAARKLPAPHAPVSSGHESARGGFLKRLFSF
                     GKKRA"
     gene            632..>1824
                     /gene="glsA"
                     /locus_tag="PLP58_23380"
     CDS             632..>1824
                     /gene="glsA"
                     /locus_tag="PLP58_23380"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR03814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004359 - glutaminase activity [Evidence
                     IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase A"
                     /protein_id="HRJ11541.1"
                     /translation="MLSPIQEYLENLHARHAPAREGAVASYIPELSKADPDWFGICIA
                     TRDGHVYEVGDTRQPFTIQSISKALAYGLALEDRGEEHVLSRIGVEPSGEAFNAISLK
                     EGSGAPFNPMINAGAIATCGQVLAKDGKTRFERLLEFLGGFAGRALDVDEAVYESESA
                     TGHRNRAIGWLLRNFGIVDENPHETLETYFRQCAIRVTCRDLALMGATLANQGVQPLT
                     QKRAIPTQYVDNVLGVMATCGMYDWSGEWIYRVGLPAKSGVGGGILAVLPGQLGIGVF
                     SPPLDAQGNSHRGIQVCMDLSRELSLHMMNPTATPRTAVRLGYHGGEVTARRRLPQSS
                     RETLRRHGSRIQVLELQGGLIFTTFEPVVRQVFQLANAGCLHIIFDLRAVVMANTVSL
                     KLLADL"
BASE COUNT          303 a          535 c          673 g          313 t
ORIGIN      
        1 tgttccgccg cgccgcgtcc ttcaaggtgt ggtaaaagct cagaaagggc atgtatgccg
       61 tgcgtcacca tgaattgtga tttcccgcag attcaaggag gccagcagat ttctctttct
      121 catccttaat ctgcgggcct ccctgaatct gtgggagaaa acccaattta caaaaggtga
      181 tgaccttcag tataaatgtc atggcgcggg gcatgagcgg tgctcaggcg cgttttttgc
      241 cgaagctgaa gagccgcttg aggaagccgc cgcgcgcgga ctcgtgaccg gagctgacgg
      301 gggcatgggg cgcgggcagt ttgcgggccg cagtgtcgcc cgcggggctg gcgggctgga
      361 ggggttcctt ttcctccgcc tgggtccacc aggcgggctg gagcaggccg acggcctgcc
      421 actcctgcgc gccggggcac cacacgaggg tgtccgcgcg gatgcgtccg gcgtcccttt
      481 cggcgcgcag gccgggcagg tcgagaggac cggcggcctc gccttgattg tcgtaatacc
      541 agttcatgag cggaggctaa acgcggcggc ggcggcgggg gaagaaaaaa atattcgttc
      601 ttacggcatc atccgtgctt ccgcgcttcg gatgctctct cccatccaag agtacctcga
      661 gaatctgcac gcccgccacg cgcccgcgcg ggagggtgcc gtggccagct acatccctga
      721 gctctcgaag gcggacccgg actggttcgg catctgcatc gcgacgcggg acgggcatgt
      781 ctatgaggtg ggtgacacgc ggcagccttt cacgatccag tccatctcca aggcgctggc
      841 gtacgggctg gcgctggagg accgcgggga ggagcatgtg ctctcgcgca tcggggtgga
      901 gccgtccggg gaggcgttca acgcgatcag cctgaaggag ggcagcggcg cgccattcaa
      961 cccgatgatc aacgcggggg cgatcgccac ctgcgggcag gtgctggcga aggacggcaa
     1021 gacacgcttt gaaaggctgc tggagtttct gggcggcttc gcgggccggg cgctggatgt
     1081 ggacgaggcg gtgtatgaga gcgagagcgc gaccgggcac cgcaaccgcg ccatcggctg
     1141 gctgctgcgg aacttcggca ttgtggatga gaacccgcac gagacgctgg agacgtattt
     1201 ccgccagtgc gccatccggg tgacgtgccg cgacctggcg ctgatggggg cgacgctggc
     1261 gaaccagggc gtgcagccgc tgacgcagaa gcgcgcgatc ccgacgcaat atgtggacaa
     1321 tgtgctgggg gtgatggcga cctgcgggat gtatgactgg tccggggagt ggatctaccg
     1381 cgtgggcctg ccggcgaaga gcggggtggg cggcggcatc ctggcggtgc tgccggggca
     1441 gctgggcatc ggcgtttttt ccccgccgct ggacgcgcag gggaacagcc accggggcat
     1501 ccaggtgtgc atggaccttt cccgcgagct gtccctgcac atgatgaacc cgacggcgac
     1561 tccgcgcacg gcggtgcggc tcggctacca cggcggggag gtgacggcgc ggcggcggct
     1621 gccgcaaagc tcacgcgaga cgctgcgcag gcacggctcg cgcatccagg tgctggagct
     1681 gcagggcggg ctgattttca cgacgttcga gccggtggtg aggcaggtct ttcaactggc
     1741 gaacgcgggc tgcctgcaca tcatcttcga cctgcgcgcg gtggtgatgg cgaacacggt
     1801 gagcctgaaa ctgctggcgg acct
//
LOCUS       DAOMYJ010000481         1811 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Prosthecobacter sp. isolate AMR_MDS_5629
            NODE_25520_length_1811_cov_19.928815, whole genome shotgun
            sequence.
ACCESSION   DAOMYJ010000481 DAOMYJ010000000
VERSION     DAOMYJ010000481.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867850
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prosthecobacter sp. (wastewater metagenome)
  ORGANISM  Prosthecobacter sp.
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Verrucomicrobiaceae; Prosthecobacter.
REFERENCE   1  (bases 1 to 1811)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1811)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.30
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:34:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,742
            CDSs (total)                      :: 4,684
            Genes (coding)                    :: 4,676
            CDSs (with protein)               :: 4,676
            Genes (RNA)                       :: 58
            tRNAs                             :: 55
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1811
                     /organism="Prosthecobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25520_length_1811_cov_19.928815"
                     /isolate="AMR_MDS_5629"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1965333"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..325)
                     /locus_tag="PLP58_23385"
     CDS             complement(<1..325)
                     /locus_tag="PLP58_23385"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HRJ11542.1"
                     /translation="MNADLLLAHLDAFVGFVRSRTGDPELAADIVQECLLKAMKADNV
                     PEEAEGVVTWFYRVLRHAIIDAHRRKTTRDSALEKLAQQWPESPSPEDEKALCQCVLR
                     LLPELP"
     gene            437..>1811
                     /locus_tag="PLP58_23390"
     CDS             437..>1811
                     /locus_tag="PLP58_23390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009960597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HRJ11543.1"
                     /translation="MKSHSSPSCCHSAPEPAAPACCHSQPKAPAPSCCHAAPAQEAHA
                     CHLHGHHPDAQVKPSATAKYFCPMCPGVESDKPGICPKCGMALERNPAFGPADAHEDD
                     AELRDMTHRFQLSALLTLPVFVTAMAHLVPAWSHAEWANGTVARWMQLILATPVVLWA
                     GWPFFERAWVSLRHRSMNMFTLIALGVGAAYAFSLAAMLAPGWFPESLADIHGRLPLY
                     FEAAAVIIVLVLLGQVLELRARARTGSAIQELLGLQPKTARLVTPEGDVDVALEAVQA
                     GQRLRVRPGEKIPVDGVVIEGSSHVDESMLTGEPEPVEKTAGAWVTGGTLNQHGSLLV
                     QAEKVGAETMLAQIVQLVGQAQRSRAPIQALADKVAAWFVPAVLAAAALTFALWLWLG
                     PQPSLAYAIANAVAVLIIACPCALGLATPMSVMVGIGRGAQMGVLIRRAEAIEKLAAL
                     NTLAVDKT"
BASE COUNT          283 a          567 c          651 g          310 t
ORIGIN      
        1 ctggcagctc gggcagcagc cgcagcacgc actggcagag ggctttctcg tcctcgggcg
       61 atggcgactc cggccactgc tgcgcgagct tttccagagc gctgtcacgc gtcgttttcc
      121 gacgatgcgc gtcaatgatg gcatgacgca gcacgcggta gaaccaggtg acgacgcctt
      181 cggcctcctc gggcacgttg tccgccttca tggccttgag caggcattcc tgcacgatgt
      241 ccgcggccag ttcgggatcg ccggtgcggg agcggacaaa gccgacgaag gcatcgagat
      301 gggcgagcag cagatcggcg ttcatattgc caaactacgc gaagagcgcg atgaattcat
      361 gctgtaacag atttcaggct cgcgcgtgag cggttcatga aggcctcaac ggtccgactc
      421 attactcatc cccatcatga aaagccattc gtcaccctcc tgctgtcact cagcgcctga
      481 acccgccgcg ccagcctgct gccatagcca gccgaaagct cccgcgccga gctgctgcca
      541 tgccgcgcct gcccaagaag cgcacgcctg ccatttgcat gggcaccacc ctgacgcgca
      601 ggtaaaaccc tccgccacgg cgaagtattt ttgcccgatg tgccccggcg tggagtccga
      661 caaaccgggc atctgcccga agtgcggcat ggcgctggaa cgcaatccgg cctttggccc
      721 ggcggatgcc catgaggatg acgcggagct gcgtgacatg acccaccggt tccaactcag
      781 cgcgctgctg acgctgccgg tctttgtcac cgccatggcg cacctcgtgc ctgcgtggag
      841 ccacgcggag tgggcgaacg gcacggtggc gcgctggatg cagctcattc tggcgacccc
      901 ggtggtgctg tgggcgggat ggccgttctt tgagcgtgcg tgggtttccc tgcggcatcg
      961 cagcatgaac atgttcacgc tcatcgcgct cggggtgggc gctgcctatg cgttcagcct
     1021 tgcggccatg ctggcgcccg ggtggttccc tgaatcgctc gcagacatcc atggccggct
     1081 gccgctgtac tttgaggcgg cggccgtgat catcgtgctg gtgctgctgg gccaggtgct
     1141 ggagctgcgg gcgcgggcgc gcacgggcag cgccatccag gagctgctgg gcctgcagcc
     1201 gaagacggcg cggctcgtca cgccggaggg ggatgtggat gtggcgctgg aggcggtgca
     1261 ggccggccag aggctgcgcg tgcgccctgg ggaaaagatc cctgtggatg gcgtggtgat
     1321 cgagggcagc agccatgtgg atgagtccat gctgacgggc gagcccgagc cggtggagaa
     1381 aaccgccggg gcgtgggtga cgggcggcac actgaaccag cacggcagcc tgctggtgca
     1441 ggcggagaag gtgggcgcgg agacgatgct ggcgcagatc gtgcagctgg tggggcaggc
     1501 gcagcgcagc cgggcgccca tccaggcgct ggcggacaag gtggcggcgt ggtttgtgcc
     1561 cgccgtgctg gccgcggcgg cgctgacctt tgcgctctgg ctgtggctcg ggccgcagcc
     1621 gtcgctggcc tatgccattg cgaatgcggt ggcggttttg atcatcgcct gcccctgcgc
     1681 gctgggcctg gccacaccga tgagcgtgat ggtgggcatc ggccgcggcg cgcagatggg
     1741 cgtgctgatc cgccgggcgg aggccatcga gaaactggcc gcgctgaaca cgctggcggt
     1801 ggacaagacc g
//
LOCUS       DAOMYJ010000482         1805 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Prosthecobacter sp. isolate AMR_MDS_5629
            NODE_25651_length_1805_cov_20.993714, whole genome shotgun
            sequence.
ACCESSION   DAOMYJ010000482 DAOMYJ010000000
VERSION     DAOMYJ010000482.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867850
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prosthecobacter sp. (wastewater metagenome)
  ORGANISM  Prosthecobacter sp.
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Verrucomicrobiaceae; Prosthecobacter.
REFERENCE   1  (bases 1 to 1805)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1805)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.30
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:34:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,742
            CDSs (total)                      :: 4,684
            Genes (coding)                    :: 4,676
            CDSs (with protein)               :: 4,676
            Genes (RNA)                       :: 58
            tRNAs                             :: 55
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1805
                     /organism="Prosthecobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25651_length_1805_cov_20.993714"
                     /isolate="AMR_MDS_5629"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1965333"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..510)
                     /locus_tag="PLP58_23395"
     CDS             complement(<1..510)
                     /locus_tag="PLP58_23395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009960205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tex-like N-terminal domain-containing protein"
                     /protein_id="HRJ11544.1"
                     /translation="MSAASELNINVTHVERIARELGVKAIQVGATAKLLADGATVPFI
                     SRYRKEATGMLDEVQIQGVKDRLEQLVALDDRRAAIVKSLEERKLMTDVLRAKIEKAE
                     TLNSLEDIFAPFRPKRRTRATIAKEKGLEPLADFIEANLATPAPDLATEAAKFINPDH
                     ENEELRVKDA"
     gene            782..>1805
                     /locus_tag="PLP58_23400"
     CDS             782..>1805
                     /locus_tag="PLP58_23400"
                     /inference="COORDINATES: protein motif:HMM:NF012311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HRJ11545.1"
                     /translation="MRTLFSKPLRVLVAVTLLLAAGVFWLGRPVNPPGNSGRSVSRLP
                     VQSTAPGLLNESPLAEGEEEFQTRASLLAGKVFSLKESGRPTSFRLALDRLHVTDAPV
                     AARGKRSMAASAAALLNEAESHAARTGRWPGLVLFPAADPPAEAGLDAEELEQWRENQ
                     KRVLTERVLISASDPGAAEQAAEKAGLRMARRPEYAPDHLIAEAESPLAALDALDALS
                     AHPAVASAEPLLLRKRGLKLLPDDPLFSKQWHLENNGQEGGKRGVDANVMEVWDEYQG
                     EGVRIAIVDDGLETSHPDLAPNVDSANHFDWNDEPADTDPSPNVLADKHGTAVAGLAG
                     ARGGNGA"
BASE COUNT          309 a          503 c          654 g          339 t
ORIGIN      
        1 ggcgtctttg acgcgcagct cctcattttc gtggtcgggg ttgatgaact tggcggcctc
       61 ggtggcgagg tccggcgcgg gggtggcgag gttggcctcg atgaagtcgg ccaggggttc
      121 gaggcctttt tccttggcga tggtggcgcg ggtgcggcgc ttggggcgga agggggcgaa
      181 gatgtcctca aggctgttga gcgtctcggc tttttcgatc ttggcgcgca gcacgtcggt
      241 catgagcttg cgctcctcca gggatttgac gatggcggcg cggcggtcgt cgagggccac
      301 gagctgctcc aggcggtctt tgacgccctg gatctgcact tcatccaaca tgccggtggc
      361 ctccttgcgg tagcgggaga tgaagggcac ggtggcgccg tcggcgagca gtttggcggt
      421 ggcgccgacc tggatggcct tcacgcccag ctcgcgggcg atgcgttcga catgagtgac
      481 gttgatgttg agttcggagg ctgcggacat ggttggtgaa agcttttgcg gcccgcacag
      541 tggcgtggcc gacgtttgag gtagcgagcg catccgagcg cggcaaggcg aagaagcccg
      601 gcgggggaaa ttaactttct tgacagcgga agcgccgaga agtggcacgc tggtcatcaa
      661 gaaagctggg ccagaagtag gccaaatcgg cgcatcgcct ggtgatccgt gatatttaat
      721 ataaatttca taattcgact tggcgaaccc cctcccggtg ggatggtacg gcatttccac
      781 catgcgcacc ctgttttcca agccgcttcg cgtcctcgtc gccgtcaccc ttttgctggc
      841 ggcaggggtg ttctggctgg gccggcctgt gaatccgccg ggaaattccg gccgctccgt
      901 ttcgcggttg ccggtgcagt ccacagcccc cggcctgcta aatgaaagcc ccttggcgga
      961 gggggaggag gagtttcaaa cccgtgcctc tttgctggcg ggcaaggtgt tcagtctcaa
     1021 ggaaagcggt cgtccaacca gcttcaggct ggctttggac aggctccatg tgactgacgc
     1081 tccagttgcg gcgaggggaa aacgctccat ggccgcaagc gcggcggctt tgctgaatga
     1141 ggcggagtct catgcggcaa ggaccgggcg ctggccgggg ttggtgctat ttccagcggc
     1201 tgatcctccg gcggaggccg ggctggacgc ggaggaactg gagcagtggc gggaaaacca
     1261 aaagcgcgtg ctgacagagc gcgtgctcat cagcgcgagt gatcccggcg ctgcggagca
     1321 ggcggcggaa aaggcgggct tgcgcatggc aaggcggccg gagtatgcgc cggaccacct
     1381 cattgccgag gcggagagtc cgctggcggc gctggacgcg ctggacgccc tttccgcgca
     1441 tcctgccgtg gcctcggcgg agcctctgct tctgcgcaaa cgcgggctaa aactgctgcc
     1501 ggatgacccg cttttttcca aacaatggca cttggaaaac aatgggcagg aaggcggaaa
     1561 aaggggtgtg gatgccaatg tcatggaggt ttgggatgag tatcaggggg agggcgtgcg
     1621 catcgccatc gtggacgacg gattggagac cagccacccc gacctcgctc cgaacgtgga
     1681 cagcgccaac catttcgact ggaatgatga gcctgccgac accgacccca gcccgaacgt
     1741 gctcgcggac aagcacggca ccgctgtggc gggactggcg ggcgcgcgcg gcggcaacgg
     1801 cgcgg
//
LOCUS       DAOMYJ010000483         1797 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Prosthecobacter sp. isolate AMR_MDS_5629
            NODE_25820_length_1797_cov_16.315155, whole genome shotgun
            sequence.
ACCESSION   DAOMYJ010000483 DAOMYJ010000000
VERSION     DAOMYJ010000483.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867850
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prosthecobacter sp. (wastewater metagenome)
  ORGANISM  Prosthecobacter sp.
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Verrucomicrobiaceae; Prosthecobacter.
REFERENCE   1  (bases 1 to 1797)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1797)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.30
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:34:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,742
            CDSs (total)                      :: 4,684
            Genes (coding)                    :: 4,676
            CDSs (with protein)               :: 4,676
            Genes (RNA)                       :: 58
            tRNAs                             :: 55
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1797
                     /organism="Prosthecobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25820_length_1797_cov_16.315155"
                     /isolate="AMR_MDS_5629"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1965333"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..219
                     /locus_tag="PLP58_23405"
     CDS             <1..219
                     /locus_tag="PLP58_23405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ11546.1"
                     /translation="TGASLEEKVRRAMQLAYARPARAEDLENARDFLTAADAALASTE
                     SDPAAREAKAWELLCQAWMMTNEFIHIR"
     gene            353..>1797
                     /locus_tag="PLP58_23410"
     CDS             353..>1797
                     /locus_tag="PLP58_23410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ11547.1"
                     /translation="MRRFFPRCLILLPALLNAGVGDPQLETDHPWYPGERALSSFERL
                     FATQARQYEQATGIRPETDHDKALAAWFFRNTHYAHAEEGKEDLWGGGFDNASMNATR
                     GYWTGLFAHGFGLCGTTHAQWTAELDALLGPCRSRVTGTRGHNSMEVFLTGGPYGEGR
                     WALLDHDLSTVIHDPEGGGLLGLDVIYKDWEKLASREHAPASQQGWLVCGLHPKDGAA
                     YAAYHTAEYRAGYAGPPPVVHLRRGETLRRWFQPGLEDGRTFVFWGRNAMTGGVPGME
                     RSRTWVNQPEAMHGSKTGTAHVNGQARYGNAVYEWRPDFAGGGYKEGVVSESAEQVTL
                     EFHTPFIIAATPPDDTPWGVYQPGCKNGLVLRGRARCPVSISTDGGATWHEAGAFHDG
                     MDLTDHVKGRRQYHLRLGAAARSLAGGGLVIRTVCQANPAVFPRLKDGGTRVTLGIGG
                     RGIASFGPEKTQAAPRIVEGAFDTPRLTLELP"
BASE COUNT          274 a          685 c          589 g          249 t
ORIGIN      
        1 accggcgcgt ccttggagga aaaagtccgc cgcgccatgc agctcgccta cgcccgcccc
       61 gcccgcgccg aggacctgga aaacgcgcgc gacttcctca ccgccgccga cgccgcgctc
      121 gcctccaccg agagcgaccc cgccgcccgc gaagccaaag cctgggagct gctctgccag
      181 gcctggatga tgaccaacga gttcatccac atccgctgac cagcggcagt cagcccggcc
      241 cggaccaggc ggcgcagccc gcatcacgaa tgccgctctt tgtctgatgc cgcgcgcccg
      301 cgccgtcaaa gacagcgcgc gagtttcaac cgggcgcggc gtattagcag ccatgcgccg
      361 ttttttcccc cgctgcctca tcctcctgcc cgccctgctc aacgccggcg tgggcgaccc
      421 gcaactcgag accgaccacc cctggtatcc gggagagcgg gcgctttcct ccttcgagcg
      481 gctcttcgcc acccaggcgc ggcagtatga gcaggccacc ggcatccgcc ccgagacgga
      541 tcatgacaag gcgctggccg cgtggttttt ccgcaacacc cactacgccc atgccgagga
      601 ggggaaggag gacctgtggg gcggcggctt tgacaacgcc tcgatgaacg ccacgcgcgg
      661 ctactggacg ggcctcttcg cgcacggctt cggcctgtgc ggcaccacgc acgcgcagtg
      721 gaccgcggag ctggacgcgc tgctcggccc ctgccgttcc cgcgtcaccg gcacgcgcgg
      781 gcacaactcg atggaggtct tcctcaccgg cggaccctat ggcgagggcc gctgggcgct
      841 gctggaccac gatctctcga cggtcattca cgacccggag ggcggcggcc tgctcgggct
      901 ggatgtcatt tacaaggatt gggaaaagct ggcctcgcgc gagcacgcgc ccgccagcca
      961 gcaggggtgg ctggtctgcg gcctgcatcc caaggacggc gccgcctacg ccgcctacca
     1021 cacggcggag taccgcgccg gttacgccgg gccgccgccc gtggtgcatc tgcgccgggg
     1081 tgagacgctg cggcgctggt tccagcccgg gctggaggac gggcgcacct tcgtcttctg
     1141 gggcaggaac gccatgaccg gcggcgtgcc cggcatggag cgctcccgca cctgggtgaa
     1201 ccagccggag gccatgcacg gctcaaaaac cggcaccgcc catgtcaacg gccaggcccg
     1261 ctacggcaac gccgtctatg aatggcggcc cgacttcgcc ggcggcgggt ataaggaggg
     1321 cgtggtcagc gagagcgcgg agcaggtgac gctggagttt cacaccccct ttatcatcgc
     1381 cgccaccccg ccggatgaca cgccgtgggg tgtttaccag ccggggtgca aaaacggcct
     1441 cgtcctgcgc ggccgcgcgc gctgccccgt ctccatctcc accgacggcg gcgccacctg
     1501 gcacgaggcg ggcgccttcc acgacggcat ggacctgacc gaccatgtca aaggccgccg
     1561 ccagtatcat ctgcgcctcg gcgcggcggc gcggtccctg gccggcggcg gcctggtcat
     1621 ccgcaccgtc tgccaggcca atcccgccgt ctttccccgc ctcaaggacg gcggcacccg
     1681 cgtcaccctc ggcatcggcg ggcgcggcat cgccagcttc gggccggaga aaacccaggc
     1741 cgcgccgcgc atcgtcgagg gcgcgttcga caccccgcgc ctcacccttg agctgcc
//
LOCUS       DAOMYJ010000484         1780 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Prosthecobacter sp. isolate AMR_MDS_5629
            NODE_26193_length_1780_cov_19.346087, whole genome shotgun
            sequence.
ACCESSION   DAOMYJ010000484 DAOMYJ010000000
VERSION     DAOMYJ010000484.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867850
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prosthecobacter sp. (wastewater metagenome)
  ORGANISM  Prosthecobacter sp.
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Verrucomicrobiaceae; Prosthecobacter.
REFERENCE   1  (bases 1 to 1780)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1780)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.30
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:34:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,742
            CDSs (total)                      :: 4,684
            Genes (coding)                    :: 4,676
            CDSs (with protein)               :: 4,676
            Genes (RNA)                       :: 58
            tRNAs                             :: 55
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1780
                     /organism="Prosthecobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26193_length_1780_cov_19.346087"
                     /isolate="AMR_MDS_5629"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1965333"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..514
                     /locus_tag="PLP58_23415"
     CDS             <1..514
                     /locus_tag="PLP58_23415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ11548.1"
                     /translation="LARAEMARAKTALRALDRRAGKLNKNWYKAAAHTFENGTPAREA
                     LERVPVLRGARMPTILCINSLKQGGQEGRQVLVDYVPRGGETATRLLLHWGVEGVDEG
                     LPHSMPLNRAGDALGPFPVGAVVQTRVEAANSAGARFCGVESLEILDPNSDRRPPKVT
                     RRGRPRGRVF"
     gene            565..>1780
                     /gene="argA"
                     /locus_tag="PLP58_23420"
     CDS             565..>1780
                     /gene="argA"
                     /locus_tag="PLP58_23420"
                     /EC_number="2.3.1.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR01890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004042 - acetyl-CoA:L-glutamate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino-acid N-acetyltransferase"
                     /protein_id="HRJ11549.1"
                     /translation="MPDNPPDLKPPTRFEDLRGILRYVPQFRQRVFVIAIDGALMHQP
                     GFGSLLQDVAVLQSLNIEVVLVFGARAQIQRLAARRGVALSNDDGMGRTDAATLDIAI
                     EAITRQASELMADLTALEMPVVVSNALAVHPAGVIDGVDQEFTGRIERVDVESLGALL
                     KADIIPVLPPLGYDGRGTTLRLNSDAVAVEVAIALKAAKVIFVSEAGLSAPDGARMAQ
                     ISVAEAREMHRRRDPRHDINLLSKLRHAAIACQEGVPRVHIVDGTQDEALLAELFSNE
                     GVGTMIHADDYQQMRKARSSDVSTLLSMMRQSMEDAALVPRTREQMLARIKDFFVLEV
                     DGNPVGCAAVHTHEEEGRRIAELACLFVRRSHKNRGHGRRLVAYAEEVARQRGGAALV
                     ALSTQAFRFFEEK"
BASE COUNT          275 a          545 c          719 g          241 t
ORIGIN      
        1 cctggcgcgc gcggagatgg cccgggcgaa gacggcgctg cgcgcgctgg accggcgcgc
       61 gggcaagctg aacaaaaact ggtacaaggc ggcggcgcac accttcgaga atggcacgcc
      121 ggcgcgcgag gcgctggagc gggtgcccgt gctgcgcggc gcgcgcatgc ccacgatact
      181 gtgcatcaac tcgctgaagc agggcgggca ggagggccgg caggtgctgg tggactatgt
      241 gccacggggc ggggagacgg ccacgcggct gctgctgcac tggggcgtgg agggcgtgga
      301 cgaggggctg ccgcactcca tgccgctgaa ccgcgcggga gacgcgctgg ggcctttccc
      361 tgttggggcg gtggtgcaaa cgcgggtgga ggcggcgaac agcgcgggcg cgcggttctg
      421 cggagtggag agcctggaga tccttgaccc gaacagtgac cggcgcccgc ccaaagtgac
      481 ccggcggggg cgcccgcgcg gccgtgtttt ctgacgggcg cgctccgggt cttgcagcgc
      541 ggcggcaatg cgcgaaactg gcgcgtgccc gacaaccctc ccgacctcaa gcccccgacg
      601 cgattcgaag acctgcgcgg cattttgcgc tacgtgccgc agttccggca gcgcgtgttc
      661 gtcatcgcca tagacggggc gctgatgcac cagccgggct tcggcagcct gctgcaggac
      721 gtggcggtgc tccagtcgct gaacatcgag gtggtgctgg tcttcggcgc gcgcgcgcag
      781 atccagaggc tggcggcccg gaggggggtg gctttgagca atgacgacgg catgggccgc
      841 acggacgcgg cgacgctgga catcgccatc gaggccatca cgcggcaggc gagcgagctg
      901 atggcggacc tgacggcgct ggaaatgccg gtggtggtga gcaacgcgct ggcggtgcat
      961 ccggcgggtg tcattgacgg ggtggaccag gagttcaccg ggcgcatcga gcgcgtggac
     1021 gtggaaagcc tgggcgcgct gctcaaggcg gacatcatcc ccgtgctgcc gccgctgggc
     1081 tacgacggac gcgggacgac gctgcggctg aactcggacg cggtggccgt ggaggtggcc
     1141 atcgcgctca aagcggcgaa ggtgatcttt gtgtccgagg cggggctgag cgcgccggac
     1201 ggcgcgcgca tggcgcagat ctcggtggcg gaggcgcggg agatgcaccg gcggcgggac
     1261 ccgcggcatg acatcaacct gctctcgaaa ctgcgccacg ccgccatcgc ctgccaggag
     1321 ggcgtgccgc gcgtccacat cgtggacggc acgcaggacg aggccttgct ggcggagctg
     1381 ttcagcaacg agggcgtggg cacgatgatc cacgcggacg actaccagca gatgcgcaag
     1441 gcgcgctcct cggacgtgag cacgctgctc tcgatgatgc ggcagtccat ggaggatgcg
     1501 gcgctggtgc cgcgcacgcg ggagcagatg ctggcgcgga tcaaggactt cttcgtgctg
     1561 gaggtggacg gcaacccggt cggctgcgcg gcggtgcaca cgcacgagga ggaggggcgg
     1621 aggatcgccg agctggcctg cctcttcgtg cggcgctcgc acaaaaaccg cggccacggg
     1681 cggcggctgg tggcctacgc ggaggaggtg gcgcggcagc gcgggggcgc ggcgctggtg
     1741 gcgctgagca cgcaggcctt ccgctttttc gaggagaaga
//
LOCUS       DAOMYJ010000485         1754 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Prosthecobacter sp. isolate AMR_MDS_5629
            NODE_26743_length_1754_cov_17.576221, whole genome shotgun
            sequence.
ACCESSION   DAOMYJ010000485 DAOMYJ010000000
VERSION     DAOMYJ010000485.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867850
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prosthecobacter sp. (wastewater metagenome)
  ORGANISM  Prosthecobacter sp.
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Verrucomicrobiaceae; Prosthecobacter.
REFERENCE   1  (bases 1 to 1754)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1754)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.30
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:34:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,742
            CDSs (total)                      :: 4,684
            Genes (coding)                    :: 4,676
            CDSs (with protein)               :: 4,676
            Genes (RNA)                       :: 58
            tRNAs                             :: 55
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1754
                     /organism="Prosthecobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26743_length_1754_cov_17.576221"
                     /isolate="AMR_MDS_5629"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1965333"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            18..977
                     /locus_tag="PLP58_23425"
     CDS             18..977
                     /locus_tag="PLP58_23425"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018969435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyltransferase
                     subunit alpha"
                     /protein_id="HRJ11550.1"
                     /translation="MKPILDFEKPVAKLRQDIEDARKKLESKPSDKLAAQIAEMEKKA
                     DALLRDIHNNLNPWQRVQISRHINRPFMLDYVRHCCSDFVEIHGDRHIGDDAAMPAGF
                     ATWDGRKIVLIGHQKGRDTKENLLRNFGSAHPEGYRKALRAMRLAEKFGLPVVSLIDT
                     PGAFPGVGAEERNIAEAIAFNLKEMMTLRTPVIAVVIGEGGSGGALGIGIADKVLMME
                     NAYYSVISPEGCAAILWKHREHAPEAASALKLSAQDLSKLGIIDGIVPEPPGGAHNNH
                     EEAAAALKEAVSAAIAGIGKTPVTDLLEARYQKFRALGKFTEN"
     gene            1014..>1754
                     /locus_tag="PLP58_23430"
     CDS             1014..>1754
                     /locus_tag="PLP58_23430"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HRJ11551.1"
                     /translation="MSRASCAAVIAITTLSSCTAGPDFALPEWKWPGRWKQGAADAPQ
                     EHPPGAWWTLFRDPALNRHVERALHGNQDLAAALARVQSARALSQIEKSAWHPQLGLL
                     NTTTYEHLSASSVGANLPAGAGLPPLERDRYRLFLDLNYELDLWGRVRRGVEGARARE
                     QAQVDLVDAQRLITAAEVARHYFLAASLDAQRKILNETVSLRQEALDLQQSRFQGGLA
                     NEMDVARARTELELTRADAVAIERQRGSI"
BASE COUNT          326 a          682 c          482 g          264 t
ORIGIN      
        1 atccgccaca ctttcccatg aagccaatcc tcgactttga aaaacccgtc gccaaactcc
       61 gccaggatat tgaggacgcg cgcaaaaagc tggagtccaa acccagcgac aagctcgccg
      121 cccagatcgc cgaaatggaa aaaaaagccg acgccctgct ccgggacatc cacaacaacc
      181 tcaacccctg gcagcgcgtg caaatctccc gccacatcaa ccggcccttc atgctcgact
      241 atgtgcggca ctgctgctcc gacttcgtcg aaatccatgg cgaccgccac atcggcgacg
      301 acgccgccat gcccgccggc tttgcaacct gggacgggcg caagatcgtt ctcatcggcc
      361 accagaaagg ccgcgacacc aaggaaaacc tgctgcgcaa cttcggcagc gcgcatcccg
      421 agggctaccg caaggccctg cgcgccatgc gcctggcgga gaagttcggc ctgcccgtcg
      481 tcagcctcat tgacaccccc ggcgccttcc ccggcgtcgg cgccgaggag cgcaacatcg
      541 ccgaggccat cgccttcaac ctcaaggaaa tgatgaccct gcgcaccccc gtcatcgccg
      601 tcgtcatcgg cgagggcggc agtggcggcg ccctcggcat cggcatcgcc gacaaggtgc
      661 tcatgatgga aaacgcctac tactccgtca tcagccccga aggctgcgcc gccattcttt
      721 ggaagcaccg cgagcatgct cccgaggccg cctccgccct caagctcagc gcccaggatc
      781 tctccaagct cggcatcata gacggcattg tgcccgagcc ccccggcggc gcgcacaaca
      841 accacgagga ggccgccgcc gcgctcaaag aagctgtctc tgccgccatc gccggcatcg
      901 ggaaaacacc cgtcaccgac ttgctcgagg ccaggtatca aaagtttcgc gctctgggaa
      961 aattcacgga gaattgacgg ggcggcgccg tgtttttcat catgcccttt tctgtgtctc
     1021 gcgcgtcttg cgcagccgtc attgccatca ccacgctcag ctcctgcaca gccggccccg
     1081 acttcgccct gccggagtgg aagtggccag gccgctggaa gcagggcgcc gcagacgcgc
     1141 cccaggagca cccccccggc gcctggtgga ccctctttcg tgatcccgca ctcaaccgcc
     1201 acgtcgagcg cgccctccac ggcaaccagg atctcgccgc cgcgctcgcc cgcgtgcagt
     1261 ccgcccgcgc cctttcccag atcgaaaaat ccgcctggca tccccagctc ggcctgctca
     1321 acaccaccac ctacgagcat ctctccgcca gcagcgtcgg cgcaaacctc cccgccggcg
     1381 ccggcctccc gcccttggag cgcgaccgct accgcctctt tcttgacctc aactacgagc
     1441 tcgatctctg ggggcgcgtc cgccgcggcg tcgaaggcgc tcgcgcccgc gagcaggccc
     1501 aggttgacct ggtggacgcc cagcgcctca tcaccgccgc cgaggtggcg cggcattact
     1561 tcctcgccgc ctccctcgac gcccagcgga agatcttgaa tgagaccgtc tccctgaggc
     1621 aggaggcgct ggacctgcag cagtcccgct tccaaggcgg actggccaat gaaatggatg
     1681 tcgcccgcgc ccgcaccgag ctggagctga cccgcgccga cgccgtcgcc atcgagcgcc
     1741 agcgcggctc catt
//
LOCUS       DAOMYJ010000486         1753 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Prosthecobacter sp. isolate AMR_MDS_5629
            NODE_26766_length_1753_cov_16.086572, whole genome shotgun
            sequence.
ACCESSION   DAOMYJ010000486 DAOMYJ010000000
VERSION     DAOMYJ010000486.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867850
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prosthecobacter sp. (wastewater metagenome)
  ORGANISM  Prosthecobacter sp.
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Verrucomicrobiaceae; Prosthecobacter.
REFERENCE   1  (bases 1 to 1753)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1753)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.30
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:34:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,742
            CDSs (total)                      :: 4,684
            Genes (coding)                    :: 4,676
            CDSs (with protein)               :: 4,676
            Genes (RNA)                       :: 58
            tRNAs                             :: 55
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1753
                     /organism="Prosthecobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26766_length_1753_cov_16.086572"
                     /isolate="AMR_MDS_5629"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1965333"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..902)
                     /locus_tag="PLP58_23435"
     CDS             complement(<1..902)
                     /locus_tag="PLP58_23435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009959730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ThuA domain-containing protein"
                     /protein_id="HRJ11552.1"
                     /translation="MSRLLPSLLALAVLLTFAPASTESRMRVLVIDGQNNHKWDITTP
                     VLKHALESSGAFSVEVSTTPAKGADAAAWEAWNPKFTDYAVVVSNYNGELWPEKVRTA
                     LDAYVLAGGGFVAVHAANNSFPEWQEYNRMIGVGGWGGRDENSGPWLYVKDGRLFRDV
                     SPGKGGAHGPQHEFVVEVVNEDHPVTRGLPKRWLHTRDELYSRLRGPAENVEILAAAR
                     SDLTAELEPSLMVIQHGKGRVFHTTLGHADYSMLCRGFYLTLQRGAEWAATGDVKLTA
                     AVPADFPTEDKTSPVALEGHPKQLP"
     gene            complement(957..>1753)
                     /locus_tag="PLP58_23440"
     CDS             complement(957..>1753)
                     /locus_tag="PLP58_23440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007416231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="LysR family substrate-binding domain-containing
                     protein"
                     /protein_id="HRJ11553.1"
                     /translation="QAHLLVTWLRWQQGRQIISLTSAGEGLLHEAAVILERMEESMRR
                     VRRAARGEEGELRLGYIGPPTQCFLGRLLAEFRRRFPNVSVHLEERTPERVWEMVAKG
                     RLSVALTRPVPGQGERVLATMPLRQEPLGVAVPKGHPLAVKKSASWESLAAEPLIVLA
                     RREGVGLHDEILAACRLAGFTPHIAHSPSLMGTVLSYVQAGFGAGIVTDSVAAASMPA
                     GLRFVALTPERSVPLVLAWNPEEDLPAANAFRTLVTEWQAGGKLWG"
BASE COUNT          287 a          627 c          562 g          277 t
ORIGIN      
        1 ggcaactgct tcgggtgacc ttcgagcgca acgggggagg tcttgtcctc ggtgggaaag
       61 tcggctggca cggcggcggt gagcttcaca tccccggtgg cggcccactc ggcgccgcgc
      121 tggagcgtga ggtaaaagcc ccggcaaagc atggaatagt ccgcatggcc cagcgtggtg
      181 tggaaaacgc ggcccttgcc gtgctggatg accatgaggc tcggctccag ctcggcggtg
      241 aggtcggacc tggcggcggc gaggatttcc acattctccg cagggccgcg caggcggctg
      301 taaagctcgt ccctggtgtg cagccagcgc ttcggcaggc cgcgtgtgac ggggtggtcc
      361 tcgttcacca cctcgaccac gaactcatgc tgcggcccgt gcgcgcctcc tttgccgggg
      421 gagacatcgc ggaaaagcct gccgtccttc acataaagcc aggggccgga gttttcatcg
      481 cgcccgcccc agccgcccac gccgatcatg cggttgtact cctgccattc gggaaaggaa
      541 ttgttcgcgg catgaaccgc gacaaagccg ccgcccgcca gcacataggc gtcaagggcg
      601 gtgcggactt tttccggcca gagctcgcca ttgtaattgc tcaccaccac ggcgtagtcg
      661 gtgaacttcg ggttccatgc ctcccatgcg gcggcgtccg cgcccttcgc tggcgtggtg
      721 gagacctcga cggaaaaagc gccgctgctc tccagcgcgt gcttgagcac gggcgtggtg
      781 atgtcccact tgtggttgtt ttgcccgtca atgacgagca ccctcatccg gctctctgtg
      841 gaggccgggg cgaaggtcag taatacggca agggcgagga gggagggcag gagtcggctc
      901 atgggcacat cgtgaagact ccagccccgc gccgcaaggg gaaaggagcg ccgctttcat
      961 ccccacagtt ttccgccggc ctgccactca gtgacgagcg tgcggaaggc attcgccgcc
     1021 ggcagatcct cctccgggtt ccatgccagc accagcggca cgctgcgctc cggggtcagc
     1081 gccacaaaac gcagccccgc cggcatggac gctgccgcca cactgtccgt gacgatgccc
     1141 gcgccgaagc ccgcctgcac atagctgagc accgtgccca tcaggctcgg gctgtgcgcg
     1201 atgtgcggcg tgaaacccgc cagccggcac gcggccagga tttcatcatg caggcccacg
     1261 ccttccctcc gcgccagcac gatcagcggt tccgcggcca gcgactccca cgacgcggat
     1321 tttttcaccg ccagcgggtg ccccttcgga accgccacgc ccagcggctc ctggcgcagc
     1381 ggcatggtgg ccagcacgcg ctccccctgg ccgggcacgg ggcgcgtcaa ggccacggac
     1441 agcctgcctt tggccaccat ctcccacacc cgctccggcg tgcgctcctc cagatgaacc
     1501 gacacatttg ggaaacgccg acggaactcg gccagcagcc ggcccaggaa acactgcgtc
     1561 ggcggtccga tgtagcccag gcgcagctcc ccctcctccc cgcgcgccgc gcgccgcacc
     1621 cggcgcatgg actcctccat ccgctccagg atcaccgccg cctcgtgcag cagcccctcc
     1681 cccgccgagg tcagcgagat gatctggcga ccttgctgcc atcgcagcca agtgacgagc
     1741 agatgcgcct ggc
//
LOCUS       DAOMYJ010000487         1745 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Prosthecobacter sp. isolate AMR_MDS_5629
            NODE_26950_length_1745_cov_12.657396, whole genome shotgun
            sequence.
ACCESSION   DAOMYJ010000487 DAOMYJ010000000
VERSION     DAOMYJ010000487.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867850
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prosthecobacter sp. (wastewater metagenome)
  ORGANISM  Prosthecobacter sp.
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Verrucomicrobiaceae; Prosthecobacter.
REFERENCE   1  (bases 1 to 1745)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1745)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.30
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:34:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,742
            CDSs (total)                      :: 4,684
            Genes (coding)                    :: 4,676
            CDSs (with protein)               :: 4,676
            Genes (RNA)                       :: 58
            tRNAs                             :: 55
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1745
                     /organism="Prosthecobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26950_length_1745_cov_12.657396"
                     /isolate="AMR_MDS_5629"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1965333"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1745
                     /locus_tag="PLP58_23445"
     CDS             <1..>1745
                     /locus_tag="PLP58_23445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ11554.1"
                     /translation="QTGVLAPPVWTAEQALLPPQARTSCATPMQSQLAWQRALQNLRP
                     EKLPALFPKPPEDCGWAWQVEMARLMMELQALTGAGGLSFADVEKLAGGDAARWRDLA
                     SLAALHVAELKRHGLEDAQEVKLRTARQPVLPEGVERVVILAAPDLPPLFDQWARACA
                     ASGVAVEVAVHAPAEMARGFDALGRPLAECWGEDAPVVTPLDDDALLLCHDAAAQAER
                     AMAELRALAPAGRVAVGVADAEAGGVLVEKLTLAEVRVFEPGGIPPRQTGLWHLLSDW
                     LALMEGGSWRCLTRLLRVPEMRAALAGSWARGCDLLARADAFTAEHLPVTLGHALEWA
                     GQKAKGAEAAGSDKTEDDAEELFFTTLRAAAALIEEMRALRLSEAARLFLLRVYGERE
                     FSPENPEDHRLTAPADAWLACCAEIEEETERFGLKPRMEEALALSLEALARAPLSEPR
                     GEVDLVLQGWLELLWESAPNLIVAGMNEEHVPGILLAHPFLPDGLRQKLGLPCQTTRF
                     ARDAFILHVLAEPRARHKGLRLLCGQWSERGDALRPSRLLFLCADAALPARVGRLFPK
                     HQEQGAPAEPARTPA"
BASE COUNT          239 a          570 c          649 g          287 t
ORIGIN      
        1 gcaaaccggc gtgctggccc cgccggtgtg gactgccgag caggcgctgc tgccgcccca
       61 ggcgcgcacc tcctgcgcga cgccgatgca gagccagctc gcctggcagc gcgctctgca
      121 aaacttgcgc ccggaaaagt tgcccgcgct ttttcccaag ccgccggagg actgcggctg
      181 ggcctggcag gtggagatgg cgcgcctgat gatggagctg caggcgctca ccggcgcggg
      241 cggactttcc tttgccgatg tggaaaagct tgcgggcgga gatgccgcgc gctggcggga
      301 tctggcttcc ctggccgctc tccacgtcgc ggaattgaaa aggcacggcc tggaggacgc
      361 gcaggaagtg aagctccgca cggcccgcca gcccgtgctg ccggagggtg tggagcgcgt
      421 ggtcatcctg gcagcgccgg acctcccgcc cttgtttgac caatgggcgc gcgcctgcgc
      481 ggcatcgggt gtcgccgtcg aggtggcagt gcatgcgccg gcggaaatgg cgcgcggttt
      541 tgacgcgctc ggacgccccc tcgcggagtg ctggggcgag gacgcgcccg tggtgacgcc
      601 gctcgatgat gacgcgctgc ttctctgcca tgacgcggcg gcccaggcgg aacgagccat
      661 ggcggaactg cgcgcgctgg cgcccgccgg gcgagtggcc gtgggtgtgg cggatgcgga
      721 ggcgggcggt gtgctggtcg agaaactgac ccttgcggaa gtgcgtgtgt ttgagccggg
      781 gggcatcccg ccgcgccaga ccggcctgtg gcatctgctt tcggactggc tcgcgctcat
      841 ggagggcggc tcatggcgct gcctcacgcg cctgctgcgc gtgccggaga tgcgcgcggc
      901 tttggcggga tcatgggcgc ggggatgcga tctgctggcg cgggcggacg ccttcaccgc
      961 ggagcatttg ccggtgactc tcggccatgc tttggaatgg gccgggcaaa aagcgaaagg
     1021 cgctgaggcg gccggcagcg acaagacgga ggatgacgcg gaggaattgt ttttcaccac
     1081 cctgcgggcg gcggccgcgc tcatcgagga aatgcgcgcc ctgcgtttgt ccgaagcggc
     1141 gcgtcttttc ctgctgcgtg tttatggaga gcgcgagttc agcccggaga acccggagga
     1201 tcaccgtctc acagcgcctg cggacgcgtg gctggcctgc tgcgcggaga ttgaggagga
     1261 gacggagcgc ttcgggctca agccccggat ggaggaggcg ctggcgctct ccctggaggc
     1321 tctggcgcgc gcgccgctct ccgagccgcg cggcgaggtg gacctcgttt tgcaaggatg
     1381 gctcgaactg ctctgggagt ccgctccgaa cctcatcgtg gccggcatga acgaggagca
     1441 tgtgcccggc atcctgctgg cgcatccgtt tctccctgac ggcctgcggc aaaagctcgg
     1501 cctgccctgc cagaccaccc ggttcgcgcg cgacgctttc atccttcacg ttctggccga
     1561 accaagagcg cggcacaaag gattgcgcct gctctgcggc cagtggagcg agcgcggcga
     1621 cgcgttgcgg ccctcgcgcc tgctctttct ttgcgctgac gccgccctgc ccgcgcgcgt
     1681 gggccgcctt tttccaaagc accaggagca gggcgcgccg gccgagccgg cccgcacacc
     1741 ggcct
//
LOCUS       DAOMYJ010000488         1744 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Prosthecobacter sp. isolate AMR_MDS_5629
            NODE_26970_length_1744_cov_26.680876, whole genome shotgun
            sequence.
ACCESSION   DAOMYJ010000488 DAOMYJ010000000
VERSION     DAOMYJ010000488.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867850
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prosthecobacter sp. (wastewater metagenome)
  ORGANISM  Prosthecobacter sp.
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Verrucomicrobiaceae; Prosthecobacter.
REFERENCE   1  (bases 1 to 1744)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1744)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.30
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:34:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,742
            CDSs (total)                      :: 4,684
            Genes (coding)                    :: 4,676
            CDSs (with protein)               :: 4,676
            Genes (RNA)                       :: 58
            tRNAs                             :: 55
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1744
                     /organism="Prosthecobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26970_length_1744_cov_26.680876"
                     /isolate="AMR_MDS_5629"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1965333"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..696
                     /locus_tag="PLP58_23450"
     CDS             <1..696
                     /locus_tag="PLP58_23450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009958870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1501 domain-containing protein"
                     /protein_id="HRJ11555.1"
                     /translation="RASLDTLHELNRQSFGAFADPEIAARIEAYEMAYRLQTSAPELM
                     DLSSEPQHILDMYGIKDVSTPGYARNCLLARRLIERGVRFVQLFHEAWDQHGNLTKAI
                     KTNCQDTDQASAALVKDLKQRGLLDDTLVVWGGEFGRTPMVQGGNDGRDHHNRGFSMW
                     FAGGGVRKGRVHGATDDLGFNAVSDPVHVHDLNATLLHLLGFDHERLTYRFQGRDFRL
                     TDVHGHLVKGMLA"
     gene            779..1684
                     /gene="folP"
                     /locus_tag="PLP58_23455"
     CDS             779..1684
                     /gene="folP"
                     /locus_tag="PLP58_23455"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009958893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004156 - dihydropteroate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HRJ11556.1"
                     /translation="MIWQARHHRIEFPRRPLVMGIVNINDDSFCGDGTLDPEQALALA
                     ARQLREGADIIDIGSESARTNRQAITVEEEVRRLLPFLKAWPELLDEHAKAPWDDAQL
                     WPPLLSINTWRPGVVTAVLPHGGDLINDISALPDGANARLCAEHGASLLIMHSVGQPK
                     VPHTHVGYESVLGTLDSFFEEKLALALQAGLSAGQIILDPGIDFAKQRDHNLAIYNHL
                     DRLHRFQRPVLLPVSRKTVIGQVLDQPDASLRDPGTIACLTAGVMRGAHIFRVHNVRA
                     AAQCVKVLWALARKKHADAAAPPAA"
BASE COUNT          301 a          637 c          523 g          283 t
ORIGIN      
        1 cgcgcctcgc tcgacacgct gcacgagctg aaccgccagt ctttcggcgc cttcgccgat
       61 cccgaaatcg ccgcgcgcat cgaggcctat gaaatggcct accgcctgca aaccagcgcc
      121 cccgaactca tggacctctc cagcgagccg cagcacatcc tggacatgta tggcatcaag
      181 gatgtgtcca cgcccggcta cgcgcgcaat tgcctccttg cccgccgcct catcgagcgc
      241 ggggtgcgat ttgtgcagct cttccacgag gcctgggacc agcatggcaa cctcaccaaa
      301 gccatcaaga ccaactgcca ggacaccgac caggccagcg ccgccctggt gaaggacctc
      361 aagcagcgcg gcctgcttga tgacaccctc gtcgtctggg gcggcgagtt cggccgcacg
      421 cccatggtcc agggcggcaa tgacgggcgc gaccaccaca accgcggctt ttccatgtgg
      481 ttcgccggcg gcggcgtgcg aaaaggccgc gtccacggcg ccaccgacga cctcggtttc
      541 aacgccgtga gcgaccccgt gcatgtccac gacctcaacg ccaccctgct gcacctgctc
      601 ggctttgacc acgagcgcct cacctaccgc ttccaaggcc gcgacttccg cctcacggat
      661 gtgcatgggc atctcgtaaa agggatgctc gcgtgagcct gcgcggggcg gggaagtgct
      721 tgccagccgc gctttgccgc gcaggtagcc gggcattccc gaagaggcag ttttcatcat
      781 gatctggcag gcacgccacc accgcatcga attcccgcgc cgtcccctgg tgatgggcat
      841 cgtgaacatc aacgacgact ccttctgcgg ggacggaacg cttgaccctg agcaggcgct
      901 ggcgctggcg gcgcggcagt tgcgcgaagg ggccgacatc atagacatcg gatccgagag
      961 cgcccgcacc aaccgccaag ccatcaccgt ggaggaggag gtgcgccgtc ttctgccttt
     1021 tctcaaagcc tggcctgagc tgctggacga gcacgccaaa gcaccgtggg atgacgcgca
     1081 gctctggccg ccgctgcttt ccatcaacac ctggcgtccc ggtgtcgtca ccgccgtgct
     1141 tccgcatggg ggagatttga tcaatgacat cagcgccctg ccggacggcg ccaacgcgcg
     1201 cctctgcgcg gaacacgggg cctccctcct catcatgcac agcgtcggcc agcccaaggt
     1261 gccgcacacg catgtcggct atgagtcggt gctcggcacg ctcgacagct tcttcgagga
     1321 aaagctggcg ctcgccctgc aggccgggct ttccgccggg caaatcatcc tcgatcccgg
     1381 catagacttc gccaagcagc gggatcacaa cctcgccatc tacaatcatc tggaccggct
     1441 gcatcgtttc cagcggcccg tgctgctgcc cgtgtcccgc aagaccgtca tcggtcaagt
     1501 gctcgaccag cccgatgcca gcctgcgtga tcccggcacc atcgcctgcc tgaccgcggg
     1561 cgtgatgcgc ggcgcgcaca tcttccgtgt ccacaatgtc cgcgccgctg cccagtgcgt
     1621 gaaggtgctg tgggcgctgg cgcgcaaaaa gcacgcggat gccgcggctc cgcccgccgc
     1681 atgaaacccg cgcgccccat cctcatcatc ggcggcgggc tcgccggcac cgcgctggcg
     1741 tggc
//
LOCUS       DAOMYJ010000489         1744 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Prosthecobacter sp. isolate AMR_MDS_5629
            NODE_26971_length_1744_cov_13.499704, whole genome shotgun
            sequence.
ACCESSION   DAOMYJ010000489 DAOMYJ010000000
VERSION     DAOMYJ010000489.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867850
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prosthecobacter sp. (wastewater metagenome)
  ORGANISM  Prosthecobacter sp.
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Verrucomicrobiaceae; Prosthecobacter.
REFERENCE   1  (bases 1 to 1744)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1744)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.30
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:34:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,742
            CDSs (total)                      :: 4,684
            Genes (coding)                    :: 4,676
            CDSs (with protein)               :: 4,676
            Genes (RNA)                       :: 58
            tRNAs                             :: 55
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1744
                     /organism="Prosthecobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26971_length_1744_cov_13.499704"
                     /isolate="AMR_MDS_5629"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1965333"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            171..932
                     /locus_tag="PLP58_23460"
     CDS             171..932
                     /locus_tag="PLP58_23460"
                     /inference="COORDINATES: protein motif:HMM:NF024579.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HRJ11557.1"
                     /translation="MSTPRARLFPAVCFLSLHLQAQEPPPATPPVPSVQPASGSQPAA
                     AAPAAPPPPVPAFNIAPVEKTGTLPPDTTILAQKAAKAFGDRDWPAARAAYQEMLLLD
                     EKNALAWANLGAVEQQAGNTQAALECFETSVKLNPHLAQSWNAIGLIHSGKGDVYMAI
                     SAFSRAIHEEPADARAHNYLAIAVKNLGWIDAAQAALMRAIELNPEYGVAHFNLALLY
                     LDQKPPAIELAKMHYQKALALGAAKDEIVERRLKE"
     gene            929..>1744
                     /locus_tag="PLP58_23465"
     CDS             929..>1744
                     /locus_tag="PLP58_23465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009959119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HRJ11558.1"
                     /translation="MSWDIAGALRGIEAAGLRRELRALAPGPGARVIFQGREVLNFSS
                     NDYLGLAASPMLRAALAGAVECEGAGSAASRLVCGTRGAHEELEQALAGFKGAEAALS
                     FTSGYAAALGALPALAGRGDTVILDKLSHACLVDAARLSGAVLRVFPHNHLGKLERLL
                     ASARGRVLVVTESVFSMDGDRAALPGIIELKERHGAWLLLDEAHAVGVLGPEGRGLAA
                     ELGLEKRVELQMGTLSKALGLCGGYIAASRAVADLLVNRARSLIYTTAPPPALA"
BASE COUNT          252 a          624 c          584 g          284 t
ORIGIN      
        1 gcattctggc gaatgcgcct acggggggcc gcaggcgcgc ctgccaggcg cggggtgcga
       61 gtttcctccg cccggattgc ccgctgccgg ttgtgtgccg ggaatgtttt ttccatccca
      121 atgacagccc gcctttgctt ccaggcgcgg tctggttgaa agtttccgcc gtgtccactc
      181 cgcgcgcccg tcttttcccc gctgtttgct ttttgagcct gcatttgcag gcccaggagc
      241 cgccccccgc cacccctccc gtcccttcag tccagcctgc ctccgggtca caacccgccg
      301 ccgccgcgcc ggcagccccg ccgccgcctg tgcccgcctt caacatcgcg cccgtggaga
      361 agaccggcac gctgccgccg gacaccacca tcctggcgca aaaggcggcc aaggcctttg
      421 gcgaccgcga ctggccggcc gcgcgcgccg cctaccagga gatgctcctg ctcgatgaaa
      481 aaaacgccct ggcctgggcc aatctcggcg ccgtggagca gcaggcaggc aacacgcagg
      541 ccgcgctcga atgcttcgag acttccgtga agctgaatcc gcatcttgcc cagtcatgga
      601 acgcgatcgg cctgatccac tccggcaagg gggacgttta catggcgatc tccgctttca
      661 gccgcgccat ccacgaggag cccgccgacg cgcgcgcgca caactacctg gccatcgccg
      721 tgaaaaacct cggctggatt gatgccgcgc aggcggcgct catgcgcgcc atcgagctga
      781 atccggagta tggcgtggcg catttcaatc tggcgctcct ctacctggac cagaagccgc
      841 ccgccatcga gctggcgaag atgcattatc aaaaggcgct ggctctgggc gccgccaagg
      901 acgagatcgt cgagcggagg ctgaaggaat gagctgggac attgcgggcg ctctgcgcgg
      961 catcgaggcc gcggggctgc gccgggagtt gcgcgcgctg gcccccggcc cgggcgcgcg
     1021 cgtgattttc cagggcaggg aagtgctgaa tttctcctcc aacgactatc tcggtctggc
     1081 cgcctccccc atgctgcgcg cggctttggc cggggcggtg gaatgcgagg gcgcgggcag
     1141 cgcggcctcg cgcctcgtct gcggcacgcg cggcgcccat gaggagctgg agcaggcgct
     1201 ggccggcttc aaaggcgcgg aggcggcgct ttccttcacc agcggttacg ccgccgcgct
     1261 gggcgccctg cccgcgctgg cgggacgcgg ggacacggtc attctcgaca agctcagcca
     1321 cgcctgcctg gtggacgcgg cgcgtctcag cggggctgtc ctgcgcgtgt tcccgcacaa
     1381 tcatctcggc aagctggagc gcctgctggc ctccgccagg ggacgtgtgc tcgtcgtcac
     1441 cgagtccgtc ttcagcatgg acggcgaccg cgccgcgctg cccggcatca tcgaactcaa
     1501 ggaacggcat ggagcctggc tgctgctcga cgaggcccac gcggtgggag tcctcggccc
     1561 ggaagggcgc ggtctcgcgg cggagctggg tttggaaaag cgcgtcgaac tgcagatggg
     1621 cacgctgagc aaggcgctcg gtctttgcgg cggctacatc gccgcgtccc gcgccgtggc
     1681 ggacctgctc gtcaaccgcg cgcgcagcct gatctacacc accgcgccgc cgcccgcgct
     1741 ggcc
//
LOCUS       DAOMYJ010000490         1740 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Prosthecobacter sp. isolate AMR_MDS_5629
            NODE_27066_length_1740_cov_13.420772, whole genome shotgun
            sequence.
ACCESSION   DAOMYJ010000490 DAOMYJ010000000
VERSION     DAOMYJ010000490.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867850
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prosthecobacter sp. (wastewater metagenome)
  ORGANISM  Prosthecobacter sp.
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Verrucomicrobiaceae; Prosthecobacter.
REFERENCE   1  (bases 1 to 1740)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1740)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.30
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:34:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,742
            CDSs (total)                      :: 4,684
            Genes (coding)                    :: 4,676
            CDSs (with protein)               :: 4,676
            Genes (RNA)                       :: 58
            tRNAs                             :: 55
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1740
                     /organism="Prosthecobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27066_length_1740_cov_13.420772"
                     /isolate="AMR_MDS_5629"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1965333"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..734)
                     /locus_tag="PLP58_23470"
     CDS             complement(<1..734)
                     /locus_tag="PLP58_23470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009963475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HRJ11559.1"
                     /translation="MKTALLLLLMPASALHPAVIETDICVFGGTSGGVAAAVQATRMG
                     RTVLLAEPGAHLGGMTSGGLSAVDIGDPRSIGGIAREYFTRLAATAGVTLAWDKPFKG
                     DGGGGPATGGAYAIEPHKAEQVFTDMAAQAGVRVLFGARLAAVKKDGPRITELLMENG
                     DILRASVFLDTTYEGDLMAKAGVTHTLTREGRDQYGETHAGIIYQDKYHPRTGHLQPG
                     PHGRVPGGQGVWDRDFPLDPYLVKGDP"
     gene            869..1642
                     /locus_tag="PLP58_23475"
     CDS             869..1642
                     /locus_tag="PLP58_23475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007418423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HRJ11560.1"
                     /translation="MDLTQCLFLSMTWLDFSWMSSSEAWIAFLTLAVMEIVLGIDNIV
                     FISILVDRLPEAERPRARFLGLGFAMLSRVALLLCISWVMQLREPLFEALGQEVSGKD
                     LILMLGGLFLLFKATVEIHHKVEGHAESQKGAAGKAALGAILVQIAILDLVFSLDSVI
                     TAVGMVEHLTVMVLAVLVSVGFMMLFAGAVSDFISRHPTVKVLALSFLLLIGTTLVAE
                     GFHMHFSKNYIYFAMAFSVFVEMLNIRMKGRMARVPGRE"
BASE COUNT          212 a          457 c          733 g          338 t
ORIGIN      
        1 gggtcgcctt tgacgaggta ggggtcgagc gggaagtcgc ggtcccagac gccctggccg
       61 ccggggacgc ggccgtgggg gccgggctgg aggtggccgg tgcgcgggtg gtatttgtcc
      121 tggtagatga tgccggcgtg ggtttcgccg tactggtcac ggccttcgcg cgtgagggtg
      181 tgggtgacgc cggctttggc catgaggtcg ccctcgtagg tggtgtcgag gaagacgctg
      241 gcgcggagga tgtcgccgtt ctccatgagc agctcggtga tgcgggggcc gtcttttttc
      301 acggcggcaa ggcgggcgcc gaagagcacc cgcacgccgg cctgggcggc catgtctgtg
      361 aagacctgct cggccttgtg cggctcgatg gcgtaggcgc cgccggtggc ggggccgccg
      421 ccgccgtcgc ctttgaaggg tttgtcccag gcgagggtga cgccggcggt ggcggcgagc
      481 cgggtgaagt attcgcgggc gatgccgccg atgctgcgcg ggtcgccgat gtccacggcg
      541 ctgaggccgc cgctggtcat gccgccgaga tgcgcgccag gctcggccag gaggacggtc
      601 ctgcccatgc gcgtggcctg cacggcggcg gcgactccgc cggaggtgcc gccgaagacg
      661 cagatgtcgg tttcgatgac ggcggggtgg agggcggacg cgggcatgag gagcaggagg
      721 agggcggttt tcatggtgct gtccgggaga cgctgcggcg gggggcggcc tttctggtca
      781 tgcggcctga ccatgagggc tgcggggcgc gggggcgggg gttttttggc gctggacttt
      841 ggcgcgggct gctgtacagg gcgcgcgcat ggacctgacg caatgccttt tcctctcgat
      901 gacgtggctg gacttttcgt ggatgagcag ctcggaggcg tggatcgcct tcctgacgct
      961 ggcggtgatg gagattgtgc tggggataga caacatcgtg ttcatttcca tcctcgtgga
     1021 ccggctgccg gaggcggagc gtccgagggc gcgctttctg gggctgggct tcgcgatgct
     1081 gtcgcgcgtg gcgctgctgc tgtgcatctc gtgggtgatg cagttgcggg agccgctgtt
     1141 tgaggcgctg gggcaggagg tgtcggggaa ggatttgatc ctgatgctgg gggggctgtt
     1201 cctgctgttc aaggcgacgg tggagatcca ccacaaggtg gaggggcacg cggagtcgca
     1261 gaagggggca gcggggaagg cggcgctggg ggccatcctg gtgcagatcg ccatcctgga
     1321 cctggtgttt tccctggatt cggtgatcac ggcggtgggc atggtggagc atctgacggt
     1381 gatggtgctg gcggtgctgg tgtcggtggg cttcatgatg ctgttcgcgg gggcggtgag
     1441 cgacttcatc agccggcacc cgacggtgaa ggtgctggcg ctgtcgttcc tgctgctgat
     1501 cgggacgacg ctggtggcgg aggggttcca catgcacttc tcgaagaact acatctactt
     1561 tgccatggcc ttctcggtgt ttgtggagat gctgaacatc cgcatgaagg gccgcatggc
     1621 gcgcgtgccg ggccgggaat gagcgcgggg gggagtgttt tacccagcca tgccggggcg
     1681 cgcaggcggc tggtgcggag cctgcgcggt ggcggggcgt gaggctcggc ggctgccgtg
//
LOCUS       DAOMYJ010000491         1728 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Prosthecobacter sp. isolate AMR_MDS_5629
            NODE_27351_length_1728_cov_19.190078, whole genome shotgun
            sequence.
ACCESSION   DAOMYJ010000491 DAOMYJ010000000
VERSION     DAOMYJ010000491.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867850
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prosthecobacter sp. (wastewater metagenome)
  ORGANISM  Prosthecobacter sp.
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Verrucomicrobiaceae; Prosthecobacter.
REFERENCE   1  (bases 1 to 1728)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1728)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.30
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:34:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,742
            CDSs (total)                      :: 4,684
            Genes (coding)                    :: 4,676
            CDSs (with protein)               :: 4,676
            Genes (RNA)                       :: 58
            tRNAs                             :: 55
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1728
                     /organism="Prosthecobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27351_length_1728_cov_19.190078"
                     /isolate="AMR_MDS_5629"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1965333"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1192)
                     /locus_tag="PLP58_23480"
     CDS             complement(<1..1192)
                     /locus_tag="PLP58_23480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ11561.1"
                     /translation="MKNTIGSYHLIRQAKPESRWKVPGVREFDPNWVVRFCLPGEQKR
                     VTARLYPICPRCLASEGNPAEVRSNCSAEGCQRDVRRWADLELAVYAKALREGDMKRK
                     QEWLAPKRYASISEVIDVYLQRGPSDRRQRVNMLSAIYEQVTGREIERAKWEELSQKM
                     LMTWAEMRQEAGRRGWLGLGAGKNMPPGGWAVLRGLAAERRLKAVDEVTVAAWNTTIQ
                     TYLVSARSIFGEKSRLKVLRDLSLPELGGFMDPLPVELPCPEGHQEIPDAVMAEVERR
                     LPSLRAEDVQLYAFFRVCEETGVRPGTLRALDTEALAVLSADEMSFWKARMAADWKMP
                     VDELCDFGGLLDIPAKKGGHAVKTPVSAEVAGLLRGLAGTSGHLLGATLVVSEGFATP
                     REAGA"
     gene            complement(1360..1433)
                     /locus_tag="PLP58_23485"
     tRNA            complement(1360..1433)
                     /locus_tag="PLP58_23485"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1399..1401),aa:Gln,seq:ctg)
BASE COUNT          378 a          643 c          410 g          297 t
ORIGIN      
        1 gcgcgccggc ctcgcgcggc gttgcgaaac cctcgctgac gaccagggtg gcgcctaaca
       61 aatggccaga tgtcccagcc aacccacgca gcaaacccgc cacctccgca gaaaccggcg
      121 tcttcaccgc atgaccaccc ttcttagccg gaatgtccag caagccgccg aaatcgcaca
      181 gctcatccac cggcatcttc cagtctgccg ccatgcgcgc cttccaaaaa ctcatctcat
      241 cagcgctcaa caccgccagt gcctccgtat ccagcgcccg caaagtcccc ggcctcaccc
      301 ccgtctcctc gcaaacgcgg aaaaacgcat aaagctgcac atcctccgcc ctcagactag
      361 gcagccgccg ctccacctcc gccatcacag catcgggtat ctcctggtga ccctccgggc
      421 agggcaactc cacaggcagc ggatccataa aaccccccag ctcaggcagc gacaaatccc
      481 gcagcacctt caagcggctc ttctccccga agatcgaccg agcagatacc agataagtct
      541 gaatcgtcgt attccacgcc gccaccgtca cctcatccac cgccttcagc cttcgctcag
      601 ccgccagccc acgcagcaca gcccatcctc caggcggcat attcttacca gcccccagac
      661 ccagccaacc cctacggcca gcctcctggc gcatctccgc ccacgtcatc agcattttct
      721 gagacagttc ctcccacttg gcccgctcaa tctcccgccc cgtcacctgc tcatagatcg
      781 ccgaaagcat attcacccgc tgccgccgat cactcgggcc gcgctgcaaa tacacgtcga
      841 tgacctcaga gatcgacgca tagcgttttg gagccagcca ttcctgcttg cgcttcatat
      901 ccccctcccg caaagccttc gcatacaccg ccagctccag atcagcccag cgcctcacat
      961 cgcgctgaca accctccgcc gagcaattag accgcacctc cgccgggttg ccctcgcttg
     1021 ccagacagcg cgggcaaatc gggtaaagtc tcgccgtcac tcgcttttgt tccccaggca
     1081 ggcaaaacct caccacccaa ttagggtcga actcacgcac cccaggcacc ttccagcgag
     1141 attcaggctt agcttggcgg atgagatgat agcttccgat agtgtttttc atagcgctag
     1201 acgcaccact agacactcct atctcataaa tcaacataaa accgcacaac aaaaaccaca
     1261 cagagaccag cacaccaaac agacccgctc aaaagttcac acccttataa ttataagctt
     1321 cctgcgctga acgccttcgt tttacaggct tcgctgtagt ggctacccct caaggactcg
     1381 aaccttgaac aacagagtca gaatctgtta tgttaccatt acaccaaggg gtagtgtgag
     1441 cggcgcgtgg agggattgtg gcgcggctgg cggggattgc aaggatttgt ctgcgcgggg
     1501 cgcgggcggg tggaaaagga cggcgtgggg cgcgaaggtg cggccttgcg gggcggagcg
     1561 ggagttgtaa gaaggggctg ccttttcacg aatctcccgc ccatgtccga ggaaccccaa
     1621 gacgcgtccg ccgagcttgc acgccgccac cgcagccacc accgccgccg gcgccgaccc
     1681 cgacctctgc gcgcatgttg cctgtaccga ggtgctgccg gccgccag
//
LOCUS       DAOMYJ010000492         1725 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Prosthecobacter sp. isolate AMR_MDS_5629
            NODE_27434_length_1725_cov_15.124551, whole genome shotgun
            sequence.
ACCESSION   DAOMYJ010000492 DAOMYJ010000000
VERSION     DAOMYJ010000492.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867850
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prosthecobacter sp. (wastewater metagenome)
  ORGANISM  Prosthecobacter sp.
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Verrucomicrobiaceae; Prosthecobacter.
REFERENCE   1  (bases 1 to 1725)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1725)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.30
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:34:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,742
            CDSs (total)                      :: 4,684
            Genes (coding)                    :: 4,676
            CDSs (with protein)               :: 4,676
            Genes (RNA)                       :: 58
            tRNAs                             :: 55
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1725
                     /organism="Prosthecobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27434_length_1725_cov_15.124551"
                     /isolate="AMR_MDS_5629"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1965333"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1725)
                     /locus_tag="PLP58_23490"
     CDS             complement(<1..>1725)
                     /locus_tag="PLP58_23490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ11562.1"
                     /translation="IDAGSLPCANPAQARQVLAGRVEDVFDARGPARAISDWLSRHAE
                     SLRPLMAREAAKLLPAGGDVIEHWLDAMLAQAVNGGMDGLAEMRDQTARDSAQGVPLW
                     LLLLGLWKPVVLLVDHLDGYYRNAEAGVAIASLLMDLVDGHGIHVLLSLNQDVWQATF
                     GHHLPSALEDRLTASQVLLRGLREADAAELLRLRLERAGVTSAESAEFATFVDVKRHF
                     IGRPVGSVSARAFLRHCARQWEIFQNSPPSPASAPEEDADTPGVFPATSELFVESPTV
                     PLVTDTVAEGLPDIFDADTTSEMKIIAEGLAEPAHALPQNKLPDILPPSPPPASEPAL
                     DFPPAQTHVAPAADAFVKLREMLHRLRETDSAHPDSGTAPSSQIPPQQAPPPPSLSSP
                     AVPPPPAPPKPDPAADLLLGRFGALRMQMHAEASSQPLDSAKLTDLVRLAGRRFPLVR
                     LSEHELPGMTGRHTLCWSLQGLEIIFGLSPMSDLPYWRTLAGFCAGRQADLADLAEKE
                     GRAAPKVKWVIFNSAHDDAAWQTLAQSGEIPAPLRDSVDLVWLEPGEIAALYAMQRII
                     KEAESGVL"
BASE COUNT          292 a          547 c          617 g          269 t
ORIGIN      
        1 ggaggacgcc gctttcggct tctttaatga tgcgctgcat ggcgtagagc gcggcgattt
       61 cccccggctc cagccaaacg aggtccacgc tgtcccgcag cggggcggga atctcgccgc
      121 tttgcgccag agtttgccag gcggcatcgt catgggctga gttgaagatg acccacttca
      181 ctttgggcgc ggcgcggcct tccttttccg ccagatcggc caggtccgcc tgacgaccgg
      241 cgcagaagcc cgccagggtg cgccagtagg gcaggtcgga catgggggac agtccgaaga
      301 tgatttccaa cccctgcaga gaccagcaca gggtgtgccg tccggtcatg cccggcagtt
      361 cgtgctccga aagcctcaca agaggaaagc gccggcccgc cagacgcacc aggtcggtga
      421 gcttggcgga gtcaagtggc tggctggacg cctcggcatg catttgcatg cgcagcgcgc
      481 cgaacctgcc cagcagcaga tcagccgcag ggtccggctt cgggggcgct ggtggtggcg
      541 gaactgcggg agaagaaagg gaaggggggg gcggcgcttg ctggggcggt atttgggaag
      601 acggcgccgt tcccgaatcg ggatgagccg agtctgtttc gcgcagccgg tgcagcatct
      661 cccgcagttt cacaaacgca tcggcggcgg gagccacatg cgtctgggcg ggcggaaaat
      721 ccagggcagg ttcggaggcg ggcggcggtg aggggggcag gatgtccggc agcttgtttt
      781 gaggcagcgc atgggctggc tcggccagtc cctcggcgat gattttcatt tcggaggtgg
      841 tgtcggcatc aaagatgtcc ggcaaacctt cggcgacggt gtccgtgacc aggggcacgg
      901 tggggctctc cacgaagagc tccgacgtgg cggggaagac accgggcgtg tccgcgtcct
      961 cctcgggggc cgacgcgggt gagggtggcg agttttgaaa aatctcccac tgccgcgcgc
     1021 agtggcgcag aaaagcgcgc gcggaaaccg agccgaccgg acgtccgatg aaatgccgtt
     1081 tcacatccac gaaagtcgcg aactcggcgg actcggcgga cgtcacgccc gcgcgctcca
     1141 accgcagccg cagcagttcg gcggcatccg cctcgcgcag gccgcgcagg agcacctggc
     1201 tggcggtgag cctgtcctcc agggcgctgg gcagatggtg tccgaaagtg gcctgccaca
     1261 catcctggtt gaggctcagc agcacatgga tgccgtggcc gtccacaagg tccatgagca
     1321 gcgaggcgat ggccacgccc gcctcggcgt tgcggtagta gccgtccaga tggtccacga
     1381 gcagcaccac gggcttccag agaccgagca gcaaaagcca cagcggcact ccctgcgcgg
     1441 agtcgcgcgc ggtctggtcg cgcatttccg ccagtccgtc catgccgccg ttcaccgcct
     1501 gggccagcat ggcgtccagc cagtgttcaa tgacatcgcc gccggcgggc agcagtttcg
     1561 cggcctcgcg cgccatgagc gggcgcaggc tctcggcatg gcgtgaaagc cagtcgctga
     1621 tggcccgggc cgggccgcgc gcgtcgaaga catcctcaac cctgcccgca agcacctgcc
     1681 gggcctgggc cgggttcgcg cacggcaggc tgcccgcgtc aatca
//
LOCUS       DAOMYJ010000493         1717 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Prosthecobacter sp. isolate AMR_MDS_5629
            NODE_27624_length_1717_cov_14.421781, whole genome shotgun
            sequence.
ACCESSION   DAOMYJ010000493 DAOMYJ010000000
VERSION     DAOMYJ010000493.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867850
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prosthecobacter sp. (wastewater metagenome)
  ORGANISM  Prosthecobacter sp.
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Verrucomicrobiaceae; Prosthecobacter.
REFERENCE   1  (bases 1 to 1717)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1717)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.30
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:34:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,742
            CDSs (total)                      :: 4,684
            Genes (coding)                    :: 4,676
            CDSs (with protein)               :: 4,676
            Genes (RNA)                       :: 58
            tRNAs                             :: 55
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1717
                     /organism="Prosthecobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27624_length_1717_cov_14.421781"
                     /isolate="AMR_MDS_5629"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1965333"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..294)
                     /locus_tag="PLP58_23495"
     CDS             complement(<1..294)
                     /locus_tag="PLP58_23495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ11563.1"
                     /translation="MTRLILAAIPESAALGAVAAAALVSAAVMWWRMQKALRIQQQQA
                     EAALIEAETTWQARLQLSEARMEESEAARAALEDTLDTARRRAAAFPAMRAHAS"
     gene            complement(291..1367)
                     /locus_tag="PLP58_23500"
     CDS             complement(291..1367)
                     /locus_tag="PLP58_23500"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HRJ11564.1"
                     /translation="MATVTHSTSWNSTPRSSFRSREDWSLKWWIMFALILSFIFHGLL
                     VMSFDKISRFLGHSTAQVTPPKLERFTIDPKLLQEQQAVRNIPDMIAPAGTQPDIQSF
                     APDLDSFDKAQMIPENREIDLTPNVKEITNFIRADDPGDQRTPGRMREDMAALLAPRE
                     LTLPDLAGAMAEMRKEVLSAPVSEKQMLLDAGALEPGSGQVDMNLLEQVKAQGGDGSS
                     ARVRGFSNLDDLLGGGGTLGASTAPILMPTDLLFDYGSDQLAEGARLSLMKLGFLIQK
                     NPDSLFIIEGHTDSFGGEQFNLELSMRRAQAVVAWLQQSLRLGSDRIQAVGMGKTKPL
                     VSPNGSVEQQSMNRRVEIKVRTRK"
     gene            1474..>1717
                     /locus_tag="PLP58_23505"
     CDS             1474..>1717
                     /locus_tag="PLP58_23505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009963109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ11565.1"
                     /translation="MSLPRLLPALALLALTASAQQTPHSFQGEARIPVGYRYLLALPE
                     GYEADTARQWPLVVFLHGAGERGTDLELLKKHGPPKL"
BASE COUNT          245 a          519 c          641 g          312 t
ORIGIN      
        1 tgaagcatgt gcacgcatcg ccgggaatgc tgcggcgcgg cggcgcgcgg tgtcgagggt
       61 gtcttcgagc gcggcgcggg cggcctcgct ttcctccatg cgcgcctcgc tgagctggag
      121 cctggcctgc caggtggtct cggcctcgat gagcgcggcc tccgcctgct gctgctgaat
      181 gcggagggct ttttgcatgc gccaccacat gacggcggcg gagacgaggg cggctgcggc
      241 gaccgcgccg agagcggcgg actccggaat ggcggcgagg atcaggcgtg tcatttcctg
      301 gtgcggactt tgatctcgac gcggcggttc atgctctgct gctcgacgct gccattgggg
      361 ctgacgaggg gcttggtctt gcccatgccg acggcctgga tgcggtcgct gccgaggcgg
      421 agggactgct ggagccaggc gacgacggcc tgggcgcggc gcatggagag ttcgaggttg
      481 aactgctcgc cgccgaagct gtcggtgtgg ccttcgatga tgaaaagcga gtccggattt
      541 ttctggatga ggaagccgag cttcatgagg ctgaggcgcg cgccctcggc gagctggtcg
      601 ctgccgtagt cgaagagcag gtcggtgggc atgaggatgg gcgcggtgct ggcgccgagc
      661 gtgccgccgc cgccgagcag gtcgtcaagg ttgctgaagc ccctgacccg cgcggacgag
      721 ccgtcgccgc cctgggcctt gacctgttcg agcaggttca tgtccacctg gccggagccg
      781 ggctccagcg cgccggcgtc gagcagcatc tgcttttcag agaccggagc ggaaaggact
      841 tctttgcgca tctcggccat ggcgccggcg aggtcgggca gggtgagttc ccggggcgcg
      901 agcagggctg ccatgtcctc gcgcatcctg ccgggcgtgc gctggtcgcc ggggtcgtcg
      961 gcgcggatga agttggtgat ctccttgacg ttcggggtga ggtctatctc ccggttttca
     1021 ggaatcattt gcgccttgtc aaagctgtcg aggtccgggg cgaaggactg gatgtcaggc
     1081 tgcgtgccgg cgggcgcgat catgtcagga atgttccgca cggcctgctg ctcctggagc
     1141 agcttcgggt caatggtgaa gcgctccagc ttcggcggtg tgacctgcgc ggtgctgtgg
     1201 cccaggaagc ggctgatttt gtcaaagctc atgaccagca gcccgtggaa gatgaaggac
     1261 aggatgagcg cgaacatgat ccaccacttc aggctccagt cctcgcgcga gcggaaggag
     1321 gagcggggcg tgctgttcca ggatgtggaa tgggtgacgg tggccatgac ccctgaggat
     1381 agcacggcgc gggccgggtc cgaaacgggt tttttctttg cgccctgccc gcgcgccttg
     1441 caaaggcgcg cgcttgcggg gcgtttcagc cgcatgtccc tgccccgcct tctccccgcc
     1501 ctcgccctgc tcgccctcac cgcctcggcg caacagacgc cgcactcctt ccagggggag
     1561 gcgaggatcc ccgtgggcta ccgctacctg ctggcgctgc cggagggcta tgaggcggac
     1621 acggcaaggc aatggccgct ggtggtcttt ctgcacggcg cgggcgagcg cggcacggac
     1681 ttggaactgc tcaaaaagca cggcccgccg aagctgg
//
LOCUS       DAOMYJ010000494         1715 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Prosthecobacter sp. isolate AMR_MDS_5629
            NODE_27682_length_1715_cov_13.398193, whole genome shotgun
            sequence.
ACCESSION   DAOMYJ010000494 DAOMYJ010000000
VERSION     DAOMYJ010000494.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867850
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prosthecobacter sp. (wastewater metagenome)
  ORGANISM  Prosthecobacter sp.
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Verrucomicrobiaceae; Prosthecobacter.
REFERENCE   1  (bases 1 to 1715)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1715)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.30
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:34:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,742
            CDSs (total)                      :: 4,684
            Genes (coding)                    :: 4,676
            CDSs (with protein)               :: 4,676
            Genes (RNA)                       :: 58
            tRNAs                             :: 55
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1715
                     /organism="Prosthecobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27682_length_1715_cov_13.398193"
                     /isolate="AMR_MDS_5629"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1965333"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..184
                     /locus_tag="PLP58_23510"
     CDS             <1..184
                     /locus_tag="PLP58_23510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ11566.1"
                     /translation="SDNGAGFDPAQPSAGNGLGNLRERAAGMKARLEITSTPGNGTAI
                     QLDVPASGRWQRRTRA"
     gene            181..840
                     /locus_tag="PLP58_23515"
     CDS             181..840
                     /locus_tag="PLP58_23515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009965765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HRJ11567.1"
                     /translation="MKHEAPITTWIVEDHDTFRRSLQRLCSPERGLGPATGFANAETM
                     LAALRAAPPQQMPDVLLLDLGLPGRGGLEIMGEVHKHAPDCRIVILTVFEDEAKISQA
                     ISAGACGYLLKTSGAGDVASAIVEAHEGGSPMSPKVAASVVKMLARLTPPAAEPVALS
                     PRERELLALLVEGLTAKEIADRLGVSIHTTGSHTKNLFFKLGVHSRAAAVARALKERL
                     V"
     gene            903..>1715
                     /gene="hisD"
                     /locus_tag="PLP58_23520"
     CDS             903..>1715
                     /gene="hisD"
                     /locus_tag="PLP58_23520"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009961074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004399 - histidinol dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HRJ11568.1"
                     /translation="MKILRHTDPDWKQTAAALNRRAEASEHVREVVAGVIQAVRARGD
                     EALRDFTERFDGVRVEDFRLPQEALDAAWEKADAALREALSAAHRNVFEFARKSLRQD
                     WFCINEQGARVGEVFHPFERVGCYVPGGTAPLVSTAIMTVTLAAAAGVPEIVVCTPCG
                     KDGKVNPGLLAALKLAGATEVHTIGGAQAIAALALGTESIRPVLKIFGPGNSYVVEAK
                     RQLFGLVSIDLLPGPSEVLILSDKTGRPEFIAADILAQAEHGKDSQAGFITDE"
BASE COUNT          289 a          621 c          546 g          259 t
ORIGIN      
        1 cagcgacaac ggcgccggct ttgacccggc ccagccctcc gccggcaacg gcctcggcaa
       61 tctgcgcgag cgcgccgccg gcatgaaagc gcggctggaa atcacctcca cacccggaaa
      121 cggcaccgcc atccagctcg atgtgcccgc cagcgggcgc tggcaacgca ggacccgcgc
      181 atgaaacacg aagcccccat caccacctgg atcgtcgaag accacgacac cttccgccgc
      241 tccctccagc gcctctgctc ccccgagcgc ggcctcggcc cggccaccgg cttcgccaat
      301 gcggagacga tgctcgccgc attgcgcgcc gcccctcctc agcagatgcc ggatgtgctg
      361 ctgctggatc tcggcctgcc cggacgcggt ggtttggaaa tcatgggaga agtccacaag
      421 catgccccgg actgccgcat cgtcatcctc accgtcttcg aggacgaggc aaagatcagc
      481 caggcgatca gcgccggtgc ctgcggctat cttttgaaaa cctccggcgc cggggatgtg
      541 gcatcagcca tcgtcgaggc gcatgagggc ggctccccca tgtccccgaa ggtggcggcc
      601 agcgtggtga aaatgctcgc ccgcctcacc ccgcccgccg ccgagccggt ggcgctctcg
      661 ccgcgcgagc gcgagctgct ggcgctgctc gtggagggcc tcacggccaa ggaaatcgcc
      721 gaccgcctgg gtgtgagcat ccacaccacc ggcagccaca cgaagaacct tttcttcaag
      781 ctcggcgtcc atagccgcgc cgccgccgtg gcgcgcgcgc tgaaggagcg ccttgtttga
      841 gccatcatcc cgcgccctgc attcttgacg ctcccggttt ctgtggaacg taagcccccc
      901 gcatgaaaat cctccgccat accgaccctg actggaagca gaccgccgcc gccctgaacc
      961 gccgcgccga ggccagcgag catgtgcgcg aagtggtggc cggcgtcatc caggccgtgc
     1021 gcgcgcgcgg agacgaggcc ctgcgtgatt tcaccgagcg cttcgacggt gtgcgcgtgg
     1081 aggatttccg cctgcctcag gaagcgctgg acgccgcctg ggagaaagcg gatgcagcgc
     1141 tgcgggaggc gctctccgcc gcgcacagga acgtctttga attcgcccgc aaaagcctgc
     1201 ggcaggactg gttctgcatc aatgagcagg gcgcgcgggt gggggaggtt ttccatccct
     1261 tcgagcgcgt gggttgttat gtgccgggcg gcaccgcgcc gctcgtctcc accgccatca
     1321 tgaccgtcac actggccgcc gcggcgggcg tgccggagat cgttgtctgc acgccatgcg
     1381 gcaaggatgg caaagtgaat ccgggtttgc tcgccgcgct gaagctggcc ggcgccaccg
     1441 aggtccacac catcggcggc gcgcaggcca tcgccgcgct ggcgctgggc acggagagca
     1501 tccggcccgt gctgaaaatc ttcggccccg gcaacagcta cgtcgtcgag gccaagcgcc
     1561 agctcttcgg cctcgtctcg atagacctgc tgcccggccc gagcgaggtg ctcattttgt
     1621 ccgacaaaac cggacggccc gaattcatcg ccgccgacat cctcgcgcag gccgagcatg
     1681 ggaaggacag ccaggccggc ttcatcaccg atgaa
//
LOCUS       DAOMYJ010000495         1706 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Prosthecobacter sp. isolate AMR_MDS_5629
            NODE_27924_length_1706_cov_19.885524, whole genome shotgun
            sequence.
ACCESSION   DAOMYJ010000495 DAOMYJ010000000
VERSION     DAOMYJ010000495.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867850
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prosthecobacter sp. (wastewater metagenome)
  ORGANISM  Prosthecobacter sp.
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Verrucomicrobiaceae; Prosthecobacter.
REFERENCE   1  (bases 1 to 1706)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1706)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.30
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:34:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,742
            CDSs (total)                      :: 4,684
            Genes (coding)                    :: 4,676
            CDSs (with protein)               :: 4,676
            Genes (RNA)                       :: 58
            tRNAs                             :: 55
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1706
                     /organism="Prosthecobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27924_length_1706_cov_19.885524"
                     /isolate="AMR_MDS_5629"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1965333"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            405..>1706
                     /locus_tag="PLP58_23525"
     CDS             405..>1706
                     /locus_tag="PLP58_23525"
                     /inference="COORDINATES: protein motif:HMM:NF021041.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pectate lyase"
                     /protein_id="HRJ11569.1"
                     /translation="MKLCLAFLLPLPLLAADALPDPAAVTAAMKKAVAAYQEKLSVHG
                     GYASGWKADLSAGITEHKESPTVISIQPPGTTTMGLALVRAWRATGEAVFLKAARAAA
                     GALMECQLSSGGWDSDFDFAQDKAKNYHLRAHVLAGDTEPGKRRSRSTLDDNKTQSAL
                     LFLLELAHLPECKDDAELHAALKYGMDAVLGAQFPNGAWPQQFSGPADPATPVKKASY
                     PAQWPREFPAVKYDGFYTLNDGNMEKLVQLMLRAHDLTGEKRYLESAKKAGEFFIMAQ
                     MPEPQPAWAQQYNFDMEPVWARKFEPPSVSGSESTGVMRALLELWTVTGEERLLRPIP
                     AALKWFEGRALPDGRYARFYELRTDKPLYFVKDTYELTYDDSNLPTHYGFKVDQAKSL
                     AGVRKLLETPREKLLADRAGPGSEKSWASKAKGAAGKAQRAL"
BASE COUNT          301 a          535 c          620 g          250 t
ORIGIN      
        1 cccgccgcca cccaccagcc cgccgccgcc agcccgcacg ccaccagagc cggcagcaca
       61 tacaccaccc cgcgcctccc cagcgcgcgc agacagtcaa cgggcggcgg ctgcgaaagg
      121 ccaggcggtg gagtcatggt ctgccaggat gcggcagcgc gcgccggagt cacgcgaaat
      181 ccacccggca tcaaccgcac tgcctgccat tctcggcggc ggggctttcc tccgccgcag
      241 gcgggtcaat accgtcagcc aggtcccccc cggcggatca ttgagccgca agtgagcgcg
      301 catcccgtgg tcaatgcccc tgccgcagca tggtgcggct gacaaaacct tctttgcatt
      361 ttgtggaaga ccggggaggg agggcggcgt ttcacgctcc tgccatgaaa ctctgcctcg
      421 cctttctcct ccccctgccc ctgctggccg ccgacgcgct gcctgacccc gccgcggtga
      481 ccgccgccat gaaaaaggct gtggcggcgt atcaagagaa gctctctgtc cacggcggct
      541 atgcatcggg ctggaaagcg gacttgagcg cgggcatcac cgagcacaag gagtcaccca
      601 cggtgatctc catccagccg ccggggacga cgacgatggg gctggcgctg gtgagggcct
      661 ggcgtgccac gggtgaggcg gtgtttctca aggcggcgcg ggcggcggcg ggggcgctga
      721 tggagtgcca gctctccagc gggggctggg acagtgattt cgacttcgcc caggacaagg
      781 cgaaaaatta ccatctgcgc gcgcatgtgc tggcggggga cacggaaccg ggcaagcgcc
      841 gctcgcgcag cactctggat gacaacaaga cgcagtcggc gctgcttttc ctgctggagc
      901 tggcgcacct gccggagtgc aaggatgacg cggagctgca cgcggcgctg aaatacggca
      961 tggacgcggt gctgggggcg cagttcccca atggcgcgtg gccgcagcag ttcagcggcc
     1021 cggcggaccc cgccactccg gtgaagaagg cttcctaccc ggcgcagtgg ccgcgggagt
     1081 ttccggcggt gaagtatgac ggcttttaca cgctcaatga cgggaacatg gagaagctgg
     1141 tgcagttgat gctgcgcgcg catgatctca caggggagaa gcgctacctg gagtcggcga
     1201 agaaggcggg ggagtttttc atcatggctc agatgcccga gccgcagccg gcgtgggcgc
     1261 agcagtataa cttcgacatg gagccggtgt gggcgcggaa gttcgagccg ccgagcgtga
     1321 gcgggagcga gagcacgggg gtgatgcgcg cgctgctgga gctgtggacg gtgacggggg
     1381 aggagcggct tttgcggccc atcccggcgg cgctgaagtg gttcgagggg cgggcgctgc
     1441 ctgacgggcg ctacgcgcgc ttctacgagc tgcgcacgga caagccgctg tatttcgtga
     1501 aggacactta cgagctgact tacgacgaca gtaacctgcc cacgcattac ggcttcaagg
     1561 tggaccaggc caagtcgctg gcgggagtca ggaagctgct ggagaccccg cgggagaagc
     1621 tgctggcgga ccgcgcgggt ccgggcagcg agaagagctg ggccagcaag gcgaagggcg
     1681 cggcgggcaa ggcgcagcgc gcgctg
//
LOCUS       DAOMYJ010000496         1701 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Prosthecobacter sp. isolate AMR_MDS_5629
            NODE_28070_length_1701_cov_16.537060, whole genome shotgun
            sequence.
ACCESSION   DAOMYJ010000496 DAOMYJ010000000
VERSION     DAOMYJ010000496.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867850
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prosthecobacter sp. (wastewater metagenome)
  ORGANISM  Prosthecobacter sp.
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Verrucomicrobiaceae; Prosthecobacter.
REFERENCE   1  (bases 1 to 1701)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1701)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.30
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:34:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,742
            CDSs (total)                      :: 4,684
            Genes (coding)                    :: 4,676
            CDSs (with protein)               :: 4,676
            Genes (RNA)                       :: 58
            tRNAs                             :: 55
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1701
                     /organism="Prosthecobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28070_length_1701_cov_16.537060"
                     /isolate="AMR_MDS_5629"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1965333"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(8..211)
                     /locus_tag="PLP58_23530"
     CDS             complement(8..211)
                     /locus_tag="PLP58_23530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ11570.1"
                     /translation="MFIQAAGALKAYLEQRRLAGATHVPLRAGMLDRLRAAREKADLR
                     KREVRRLVEATMRRRRRSIRTAM"
     gene            complement(316..870)
                     /gene="tadA"
                     /locus_tag="PLP58_23535"
     CDS             complement(316..870)
                     /gene="tadA"
                     /locus_tag="PLP58_23535"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018970442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008251 - tRNA-specific adenosine
                     deaminase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0002100 - tRNA wobble adenosine to inosine
                     editing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA adenosine(34) deaminase TadA"
                     /protein_id="HRJ11571.1"
                     /translation="MTENPPLLEPPSDDFFMREALRQARKAAKQGEVPIGAVIVHQGR
                     VIARAWNQVETLKDATAHAEMLALTQAESAMEDWRLTECDLYVTKEPCPMCAGALVHC
                     RVRRVIFGCADPKGGGAGGFWNLLQAPNLNHRCEITPGVLAEESVALLKAFFAEARQR
                     RAEGQAHAKGLKPGAEDGMVFDGP"
     gene            complement(939..1481)
                     /locus_tag="PLP58_23540"
     CDS             complement(939..1481)
                     /locus_tag="PLP58_23540"
                     /inference="COORDINATES: protein motif:HMM:NF018028.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HRJ11572.1"
                     /translation="MSTEFNPYAPPKTAELGAPVAQDTKRPLAARWRRIVAYLLESVI
                     VTALIVPAQYFAGTFQREMERQESGGSSIGYHPEIVLWTAVALLFMVAINWNLLANGQ
                     TIGKLLLNIRIDRVEGGPCERGRIIFRRMVVMQIFYLIPVINIVFIILDSMLMIFRND
                     KRTLHDMIAGTKVVDMRQTA"
BASE COUNT          294 a          566 c          531 g          310 t
ORIGIN      
        1 ttgccgatca catagcagtc cggatcgagc gccgtctgcg ccgcatcgta gcctccacca
       61 ggcgccgcac ctcccgcttg cgcaggtccg ccttctcgcg cgccgcgcgc aggcggtcca
      121 gcatcccggc gcgcagcggc acatgcgtgg cgcctgccag cctgcgctgc tccagatagg
      181 ctttcaatgc acccgccgct tgaatgaaca tgcgccatcc ttgcgccagc ccgcccggag
      241 gctcaagcgc ggatttatgg ggcgcgggca tcctgcctgc gcggacaggc gggacgcctg
      301 ttccccgtgc cggcctcacg gcccgtcaaa aaccatgccg tcctccgcgc cgggcttcag
      361 gcccttggca tgggcctggc cctccgcccg ccgctggcgc gcctcggcga aaaaggcttt
      421 taaaagcgcc acgctctcct ccgccagcac gccgggtgtg atctcgcacc ggtggttgag
      481 gttcggcgcc tggagcaggt tccagaaacc gcccgcgccg ccgcccttgg ggtccgcgca
      541 gccgaagatc acccgccgca cccggcaatg caccagcgcg cccgcgcaca tcgggcaggg
      601 ttccttggtc acatacaggt cgcactccgt cagccgccag tcctccatgg cgctctccgc
      661 ctgggtcagc gccagcatct ccgcgtgcgc cgtggcatcc ttcagcgtct ccacctggtt
      721 ccacgcgcgg gcgatgacac gcccctggtg gacgatcacc gcgccgatgg gcacctcccc
      781 ctgcttggcc gccttgcgcg cctggcgcag cgcctcccgc atgaaaaaat catccgaagg
      841 cggctcgagc aggggcgggt tctcagtcat ggcgcgcatc ctcccacgcc ccggctggat
      901 gcgcaaaccg gcaggcggga ggaatacgat gcgcggggtc aggcggtctg tctcatatcc
      961 accactttgg ttcccgcgat catgtcgtgc agcgtgcgct tgtcgttgcg gaagatcatc
     1021 agcatgctgt ccaagatgat gaagacgatg ttgatcacag gaatgagata aaaaatctgc
     1081 atgaccacca tgcggcggaa gatgatgcgc ccgcgctcgc atgggccgcc ttccacacgg
     1141 tctatgcgga tattcaacaa gagcttcccg atcgtctgtc cgttggcgag gagattccaa
     1201 ttgatcgcca ccataaagag cagcgctacg gcagtccaga gaacgatctc cggatgatat
     1261 ccaatggacg agcctccaga ttcctgccgc tccatttcgc gctgaaaagt gcccgcaaaa
     1321 tactgcgccg gcacgatgag ggccgtgact atcaccgatt ccagcagata tgcgacgatt
     1381 ctgcgccacc gtgccgcaag cggccgttta gtatcctgcg cgacgggcgc gccgagctcg
     1441 gcggtctttg gcggggcgta ggggttgaat tcggtgctca tcggggtggg tagttatgaa
     1501 ggcacccgaa ggtggctctg atttccagag tggcattgga aaggcaaaat gccatttttc
     1561 ccctgttttt gaccagggcg gggctagggt gggggcatgg aggagcctga gccggtgcgc
     1621 ataccgatca cgaatgagct ggatctgcat gctttccgcc cggatgaggc gggggagctg
     1681 ctggtggatt acttcgcgga g
//
LOCUS       DAOMYJ010000497         1685 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Prosthecobacter sp. isolate AMR_MDS_5629
            NODE_28494_length_1685_cov_11.500613, whole genome shotgun
            sequence.
ACCESSION   DAOMYJ010000497 DAOMYJ010000000
VERSION     DAOMYJ010000497.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867850
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prosthecobacter sp. (wastewater metagenome)
  ORGANISM  Prosthecobacter sp.
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Verrucomicrobiaceae; Prosthecobacter.
REFERENCE   1  (bases 1 to 1685)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1685)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.30
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:34:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,742
            CDSs (total)                      :: 4,684
            Genes (coding)                    :: 4,676
            CDSs (with protein)               :: 4,676
            Genes (RNA)                       :: 58
            tRNAs                             :: 55
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1685
                     /organism="Prosthecobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28494_length_1685_cov_11.500613"
                     /isolate="AMR_MDS_5629"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1965333"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..917
                     /locus_tag="PLP58_23545"
     CDS             <1..917
                     /locus_tag="PLP58_23545"
                     /inference="COORDINATES: protein motif:HMM:NF024118.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HRJ11573.1"
                     /translation="PRPRNPRRALARRAPAAKKERMVRLTVLGSGSSGNSALITTGRA
                     TLLIDAGLSAKQIQVRLEAAGHTLDQLDGILLTHEHQDHTGALEVLSGRLPLRLYCTP
                     LTQEHLLGTLKFRSPPAWKLISTGSPFEVRDLRIESFPVPHDAVDPVGYVIADEESRL
                     GVLSDVGFVTNLIKDRLRGSDSLFVEANYDTQLIEADTKRPWATKQRIMSRHGHLSND
                     QAAELVEALAHPGLRHVILGHLSDDCNDPARAIRRIQEPLHRLGIKDTRVLCAERRAC
                     TETLEVAHRRQVHAPAPDPAAAEQMRLL"
     assembly_gap    1186..1285
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          275 a          611 c          468 g          231 t
ORIGIN      
        1 ccccgcgccc gcgaaacccg cgccgcgccc ttgccagacg cgcccccgcc gctaagaaag
       61 agcgcatggt acgactcacc gtcctcggca gcggcagctc cggcaactcc gccctcatca
      121 ccacgggccg cgccaccctg ctcatagacg ccggcttgag cgcgaagcaa atccaggtcc
      181 gcctcgaagc cgccggccac accctcgacc agctcgacgg catcctcctc acccacgagc
      241 accaggacca caccggcgcg ctcgaagtcc tcagcggcag actccccctc cgcctctact
      301 gcacccccct cacccaggag cacctgctcg gcaccctcaa attccgctcc ccccccgcct
      361 ggaagctcat ctccaccggc agccccttcg aagtccggga cctccgcatc gagagcttcc
      421 ccgtcccgca tgacgccgtg gaccccgtcg gctacgtcat cgccgacgag gagtcccgcc
      481 tcggcgtcct cagcgacgtc ggcttcgtca ccaacctcat caaagaccgc ctgcgcggct
      541 ccgacagcct cttcgtcgag gccaactacg acacccagct catcgaagcc gacaccaagc
      601 gcccctgggc caccaagcag cgcatcatgt cccgccacgg ccacctctcc aacgaccagg
      661 ccgccgagct cgtcgaagcc ctcgcccacc ccggcctgcg ccacgtcatc ctcggccacc
      721 tcagcgacga ctgcaacgac cccgcccgcg ccatccggcg catccaggag cccctccacc
      781 gcctcggcat caaagacacc cgcgtcctct gcgccgagcg ccgcgcctgc accgagaccc
      841 tcgaagtcgc ccaccgccgc caggtccacg cccccgcccc ggaccccgcc gccgccgagc
      901 agatgcgcct cttgtaaaaa gcaccccgcc cgcgccggtg caggcgcatt cgccggaatg
      961 cgggggatgt gcgtggcggc gggagtcaaa cccacgccct tgcgggcgcg gctacggccc
     1021 cgtgtagaag cattcgtcag aatgcagggg atgtgcgtgg cggcgggagt caaacccgcg
     1081 ctttgacaag cgcggctacg gccccgtgta gaagcattcg tcagaatgcg ggggatgtgc
     1141 gtggcggcgg gagtcaaacc cgcgctttga caagcgcggc tacggnnnnn nnnnnnnnnn
     1201 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1261 nnnnnnnnnn nnnnnnnnnn nnnnnccccg tgtagaagca ttcgtcagaa tgcgggggat
     1321 gtgcgtggcg gcgggagtca gacccgcgct ttgacaagcg cggctacggc cccgtgtagg
     1381 cgcattcgcc agaatgcggg cttgggtgtg gcggcgggag tcaaacccgc gcttgggcgg
     1441 gcgcgcctgc gggggcgtgt ctactgaagg tcatctcctt ttgtgaattg ggttttctcc
     1501 cacagattca aggaggcccg cagattcagg atgagaaaga gaaatctgcc ggcctccttg
     1561 aatctgtggg aaatcaccct tcatggtgac gcacggcata ttctggctcc aggggcgcgt
     1621 cgccggaatg atggaggccg ggtgtggcgc agcccgcccg gcttttggga ggcgtcgttt
     1681 cgcat
//
LOCUS       DAOMYJ010000498         1681 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Prosthecobacter sp. isolate AMR_MDS_5629
            NODE_28582_length_1681_cov_21.028290, whole genome shotgun
            sequence.
ACCESSION   DAOMYJ010000498 DAOMYJ010000000
VERSION     DAOMYJ010000498.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867850
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prosthecobacter sp. (wastewater metagenome)
  ORGANISM  Prosthecobacter sp.
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Verrucomicrobiaceae; Prosthecobacter.
REFERENCE   1  (bases 1 to 1681)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1681)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.30
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:34:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,742
            CDSs (total)                      :: 4,684
            Genes (coding)                    :: 4,676
            CDSs (with protein)               :: 4,676
            Genes (RNA)                       :: 58
            tRNAs                             :: 55
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1681
                     /organism="Prosthecobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28582_length_1681_cov_21.028290"
                     /isolate="AMR_MDS_5629"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1965333"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1314)
                     /locus_tag="PLP58_23550"
     CDS             complement(<1..1314)
                     /locus_tag="PLP58_23550"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007415821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HRJ11574.1"
                     /translation="MDTPPSENPAANIFVAALDHALEDRETFLTRACGDDAALLAEVR
                     AMLAAHDAMPAGFLDGPAADPLVTLKVNPGVRLVPDGLPLAEQPGDRIGPYKLLQEIG
                     EGGFGTVWMAEQDRPVRRRVALKIIKLGMDTKEVVARFEQERQALAMMDHPNIARVFD
                     AGATEQGRPFFVMELVRGVKITDYCDEQGLSNEERIGLFIEVCQAVQHAHQKGIIHRD
                     LKPSNILVTVNDGRPVPKVIDFGVAKATQGRLTDGTLFTQFEQMIGTPLYMSPEQAEM
                     TSLDVDTRSDIYSLGVLLYELLTGRTPIDMATMAKAGMGEIRRLIREADPPRPSQRLK
                     TLDGNELTSAAKRRHTDAARLPGALRGDVDWIVMKCLEKDRQRRYDTANGLALDLQRH
                     LKNEVITARPPTTAYLLGKLIRRNKVAFAAGLAIAASLVIGIVASL"
     gene            complement(1434..1568)
                     /locus_tag="PLP58_23555"
     CDS             complement(1434..1568)
                     /locus_tag="PLP58_23555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ11575.1"
                     /translation="MKAALEGRTGSAAASAASLGEFKLRVQAVEPPAPLPRITPLNRA
                     "
BASE COUNT          270 a          555 c          563 g          293 t
ORIGIN      
        1 caggctcgcc acgatgccga tgaccagcga cgcagcgatg gcgagaccgg cagcgaaggc
       61 gactttgttg cgccggatca gcttgcccag gagataggcc gtggtgggcg ggcgcgcggt
      121 gatgacctcg ttcttgagat ggcgctgaag gtcgagcgcg aggccgttgg cggtgtcgta
      181 gcggcgctgg cgatcttttt ccaggcactt catcacgatc cagtccacat ccccgcgcag
      241 cgcgcccggc aggcgggcgg cgtcagtgtg gcggcgcttc gcggcggagg tcagctcgtt
      301 gccgtcgagc gtcttgaggc gctgggaggg ccgcggcgga tcggcctcgc ggatgaggcg
      361 gcggatttca cccatgccgg cctttgccat ggtggccatg tcgatcggcg tgcggccggt
      421 gagcagttcg tagagcagca cacccagcga gtagatgtcg ctgcgcgtgt ccacatccag
      481 cgaggtcatc tccgcctgct ccgggctcat gtagagcggc gtgccgatca tctgctcgaa
      541 ctgcgtgaag agcgtgccgt cggtgaggcg gccctgggtg gccttcgcca cgccgaaatc
      601 aatcaccttc ggcaccggcc tgccgtcgtt gacggtgacg aggatgttcg agggcttgag
      661 gtcacggtgg atgatgcctt tttgatgggc gtgctgcacc gcctggcaga cctcgatgaa
      721 aagcccgata cgctcctcat tggaaagccc ctgctcatcg cagtaatcgg tgatcttcac
      781 gccgcgcacg agttccatga caaagaaggg ccgcccctgc tccgtggccc cggcatcgaa
      841 gacgcgggcg atgttcggat ggtccatcat cgccagcgcc tgccgctcct gctcaaagcg
      901 ggccacgacc tcctttgtgt ccatgcccag cttgatgatc ttcagcgcca cccgccgccg
      961 caccggcctg tcctgctccg ccatccacac cgtgccgaag ccaccctcgc cgatctcctg
     1021 cagcagcttg tagggcccga tgcggtcgcc cggctgctcg gccaaaggca gaccatcggg
     1081 gacgaggcgc acgccgggat tcaccttgag tgtgaccagc gggtcagccg ccggaccatc
     1141 cagaaagcct gccggcatgg catcgtgcgc cgccagcatt gcgcgcacct cggccagcag
     1201 cgcggcatca tcgccgcaag ccctcgtgag aaatgtctcc cggtcctcca acgcgtggtc
     1261 cagcgccgcg acaaagatgt tcgcggcagg gttttccgaa ggcggcgtgt ccatggcagt
     1321 gctcatacaa cctgctcatg cgagaaagcg cgaggaaaag gatcagaaga tttgacggac
     1381 gctgttccgg cggcgtcatc tgccctgtct gagcaagcgg gagcccgccc cgctcatgcg
     1441 cggttgagcg gcgtgatgcg cggcagcggc gccggcggct ccacggcctg cacacgcagt
     1501 ttgaattcgc ccaatgacgc cgcgctggcc gcggcggagc cggtgcgccc ttcgagcgcg
     1561 gccttcacac cgcccgggtc ggcggattgg gaatgccgcc gccctgagag gctggcatca
     1621 ggcgagatag gatccggcga tgtcgccggc catctggcgc gcctttttca agcctccatg
     1681 c
//
LOCUS       DAOMYJ010000499         1680 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Prosthecobacter sp. isolate AMR_MDS_5629
            NODE_28609_length_1680_cov_19.472615, whole genome shotgun
            sequence.
ACCESSION   DAOMYJ010000499 DAOMYJ010000000
VERSION     DAOMYJ010000499.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867850
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prosthecobacter sp. (wastewater metagenome)
  ORGANISM  Prosthecobacter sp.
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Verrucomicrobiaceae; Prosthecobacter.
REFERENCE   1  (bases 1 to 1680)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1680)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.30
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:34:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,742
            CDSs (total)                      :: 4,684
            Genes (coding)                    :: 4,676
            CDSs (with protein)               :: 4,676
            Genes (RNA)                       :: 58
            tRNAs                             :: 55
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1680
                     /organism="Prosthecobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28609_length_1680_cov_19.472615"
                     /isolate="AMR_MDS_5629"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1965333"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..220
                     /locus_tag="PLP58_23560"
     CDS             <1..220
                     /locus_tag="PLP58_23560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ11576.1"
                     /translation="VARARRPVIEMDMEAMRRKNPKNDPVVDLMRDEVDPILDKISAH
                     GMHSLSEEERRTLERASRRISSKTGGNS"
     gene            283..1446
                     /locus_tag="PLP58_23565"
     CDS             283..1446
                     /locus_tag="PLP58_23565"
                     /inference="COORDINATES: protein motif:HMM:NF012318.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HRJ11577.1"
                     /translation="MESLRRLTFAITVFLVAFLVFSFLKKDEGGYGLLNLLRGEKPGA
                     TLTPPAAPKIQLDEIPLLSALDGEFAKLSAAVLPSVVSVTTRTVRRGQAGWHPFFGMV
                     AGRPQVIPGLGSGAIISKEGHVVTNYHVIADVSEVMVTTNDNKQHPARILGASRERDI
                     ALLKIEGEGKDFPALSFANSDEAKVGQIVFAVGNPFGLSGTVTQGIISARDRHLSDSQ
                     LDYLQTDTVINPGNSGGPLVNIRGEIIGVNVAIYQGDQNVRAWQGVGLAVPANDAKAV
                     VEAVLKQGPASDNGYLGLELSAEPVLLDRGLGGTGIGAFITDLDPGSPAAQSGLQPGD
                     VITTLNGLGFDSPAALLKAIRSLKPGSQARVALVRDGRMAEITVTIGARPDAR"
     gene            complement(1464..>1680)
                     /locus_tag="PLP58_23570"
     CDS             complement(1464..>1680)
                     /locus_tag="PLP58_23570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018970654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SDR family NAD-dependent epimerase/dehydratase"
                     /protein_id="HRJ11578.1"
                     /translation="PGEFTMIELAELVLKKVGGKSKLTFHPLPSDDPRQRKPDISLAK
                     SHLGWEPRVPLEEGIERTVAYFKTVVG"
BASE COUNT          316 a          516 c          553 g          295 t
ORIGIN      
        1 cgtggcacgc gctcgcaggc cggtgatcga gatggacatg gaggccatgc gccggaaaaa
       61 tccgaaaaac gaccctgtcg tggacctcat gcgcgacgag gtggacccca ttctcgataa
      121 aatcagcgcc cacggcatgc acagcctcag cgaggaggag aggcgcaccc tggagcgcgc
      181 cagccggcgc atcagcagca agaccggcgg caactcctga gccgtgacaa atcctccgcc
      241 gcgtcttgcc tagcctgcat gaccgcgcat gatagcaggc gcatggaatc cctccgccga
      301 ctcactttcg ccatcacggt ttttttggtc gcctttcttg tcttctcttt tttgaagaaa
      361 gatgaagggg gttacggcct gctgaacctc ctgcgcgggg agaagcccgg cgccaccctg
      421 acgccgccgg cggctccgaa gatccagctt gacgagatac cgctgctctc cgccctggat
      481 ggcgagtttg ccaaactctc cgccgccgtc ctgccctcgg tggtgagcgt gacgacgagg
      541 accgtgcgcc gcgggcaggc gggctggcac ccgtttttcg gcatggtggc agggcgcccg
      601 caggtgatcc cgggcctcgg ctcgggggcc atcatttcca aggaagggca tgtggtgaca
      661 aactaccacg ttatcgcgga tgtgagcgag gtgatggtca ccaccaacga taacaagcag
      721 catccggccc gcatcctcgg cgccagccgg gaaagggaca tcgcgctgct gaagatcgag
      781 ggggagggca aggatttccc tgctctcagt tttgccaact ccgacgaggc aaaagtgggg
      841 cagattgtat tcgccgtggg caatcccttc ggtctcagcg gcacggtgac gcagggcatc
      901 ataagcgcgc gggaccggca cttgagcgac agccagctcg actacttgca aaccgacacc
      961 gtgatcaacc ccggcaactc cggcggcccg ctggtgaaca tcagagggga gatcatcggc
     1021 gtgaacgtgg ccatttacca gggtgaccaa aacgtgcgcg cctggcaggg tgtgggcctg
     1081 gccgttccgg cgaacgatgc caaagcggtg gtggaagcgg ttttgaagca ggggccggca
     1141 tcggacaatg gctatctggg tctggaactg agcgcggagc cggtgctgct ggatcgcggc
     1201 cttggtggaa ccggcatcgg agctttcatc acggacctgg atcccggctc ccccgccgcg
     1261 cagagcgggc ttcagccagg ggatgtgatc acaacgctca acggactggg ttttgattca
     1321 ccggcggctt tgctcaaagc catacgctcg ctcaagccgg gcagccaggc gcgagtcgcg
     1381 ctcgtgcgcg acggacgcat ggcggagatc accgtcacta tcggcgcgcg tccggacgct
     1441 cgttgaggaa gcctgtcacc ggctcaacca accacggtct tgaaataggc gacggtgcgc
     1501 tcgatgccct cctccagcgg cacgcgcggc tcccagccga ggtggctttt cgccagcgag
     1561 atgtcgggct tgcgctggcg cgggtcatcg gagggaagcg ggtggaaggt gagttttgat
     1621 ttgccgccga cttttttaag caccagttcc gccagctcga tcatggtgaa ctcgccgggg
//
LOCUS       DAOMYJ010000500         1680 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Prosthecobacter sp. isolate AMR_MDS_5629
            NODE_28611_length_1680_cov_16.151385, whole genome shotgun
            sequence.
ACCESSION   DAOMYJ010000500 DAOMYJ010000000
VERSION     DAOMYJ010000500.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867850
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prosthecobacter sp. (wastewater metagenome)
  ORGANISM  Prosthecobacter sp.
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Verrucomicrobiaceae; Prosthecobacter.
REFERENCE   1  (bases 1 to 1680)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1680)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.30
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:34:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,742
            CDSs (total)                      :: 4,684
            Genes (coding)                    :: 4,676
            CDSs (with protein)               :: 4,676
            Genes (RNA)                       :: 58
            tRNAs                             :: 55
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1680
                     /organism="Prosthecobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28611_length_1680_cov_16.151385"
                     /isolate="AMR_MDS_5629"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1965333"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(387..785)
                     /locus_tag="PLP58_23575"
     CDS             complement(387..785)
                     /locus_tag="PLP58_23575"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013964.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease signature motif containing
                     protein"
                     /protein_id="HRJ11579.1"
                     /translation="MSLPAPVKTAVRERAADRCEYCRMRHRWEPLNVYHVEHVVARQH
                     RGEDTLSNLAFACPQCNLNKGPNLSSRDPDSDEVVLLFHPRQQRWEDHFEMREGRISG
                     KTGTSRTTVFLLDMNADHRVELRSENLDEW"
     gene            complement(782..1036)
                     /locus_tag="PLP58_23580"
     CDS             complement(782..1036)
                     /locus_tag="PLP58_23580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ11580.1"
                     /translation="MDTRTFDPAFGRVTDCMNREALEALVNLRAGAEQEARMAVLGEK
                     ANEGTLTREERKEYEEKILFGSFFGILQAKARKKLKALAE"
     gene            complement(1161..>1680)
                     /locus_tag="PLP58_23585"
     CDS             complement(1161..>1680)
                     /locus_tag="PLP58_23585"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HRJ11581.1"
                     /translation="AFLGSMDWHANVDAILHFAAHTLPLIKKSAPGTRVILIGRNPPP
                     ALRALAAADPAIEVTGTVDDVRPHLARASIMILPLRVGGGTRIKVFEGMAAGLALVSS
                     RIGVEGLPVEDGVHALLADEPAAFAAAVLSLIQDSDRRSRMARTARQWVEAGFSWDRA
                     ARQFLELCGSVL"
BASE COUNT          274 a          487 c          614 g          305 t
ORIGIN      
        1 atgcggaatg cggaatgcgg aatgcggaat gcggaatgcg gaatgcggaa tgcgggcgcg
       61 cgtggaggaa agcgccagtt ttcccagggc gcgggaggcg ggtgaaggat gccggcagtt
      121 gtgcggctca tccttccact tgccggaatc atcccggcag ggcgtccgac gccccagttg
      181 ccggcctgga cgttgttgtt ccgttgcagc gcggtgttgc aggcgtttgg aggctgaggg
      241 gtggcgcggt gcgggggtga ggtttggaac atgtgccgcg tccccccagc gcggcgcgct
      301 gaggataatg cgcccagcct ggggccgggc gcaggttttt gcgccttggc actacaccag
      361 ccggcagatg gagccggggg ggggcgtcac cactcgtcga gattttcaga cctcaactcc
      421 accctgtggt cggcattcat gtccagcaag aagactgtcg tcctgctggt cccggtcttg
      481 ccggaaattc ggccttcgcg catctcaaag tgatcctccc agcgctgttg gcggggatgg
      541 aaaagcagga ccacttcgtc actgtccgga tcacggctgc tcaaattcgg ccctttgttc
      601 aaattgcatt ggggacaggc gaaagccagg ttcgacaggg tgtcctctcc gcgatgctgg
      661 cgggcgacga catgttcgac atggtaaacg ttgagcggtt cccagcggtg gcgcatgcgg
      721 cagtattcgc agcggtccgc cgcccgttca cggacggctg ttttgaccgg agcgggcagg
      781 ctcattccgc cagcgccttg agttttttcc gcgccttggc ctggaggatg ccaaaaaagc
      841 tgccgaacaa aatcttttcc tcgtactctt tccgctcctc gcgagtcagc gttccctcat
      901 tcgctttctc acccaggacg gccatgcgcg cttcctgctc agcgccggcc cgcagattta
      961 caagggcttc cagggcttcc cggttcatgc agtcagtcac gcgaccgaag gcagggtcaa
     1021 acgttctcgt gtccatgcgg ggattgattc cgcagccagc cggtttcgca aagggatttt
     1081 cagcgcaaaa gcactccgcc atgcggctcc atcagcgtgc aggccgtgtc ttcgccgctg
     1141 atggcgggcg cgcagccggc tcacaggacg ctgccgcaga gttcgaggaa ctggcgggcg
     1201 gcgcggtccc aactgaagcc ggcctccacc cactggcggg cggtgcgggc catgcgggag
     1261 cggcggtcgg agtcctggat aaggctgagg acggcggcgg cgaaggcggc gggctcgtcg
     1321 gcaagcaggg cgtggacgcc gtcctcgacg ggcaggccct cgacaccgat gcgggaggag
     1381 acgagggcga gtccggcggc catgccttcg aagactttga tgcgggtgcc gccgccgaca
     1441 cggaggggca ggatcatgat ggaggcgcgg gccaggtgcg ggcgcacgtc gtccacggtg
     1501 ccggtgacct caatggcggg gtcggcggcg gcgagggcgc gcagggctgg aggcgggttc
     1561 cgcccgatga ggatgacgcg ggtgccgggc gcggattttt tgatgagggg cagggtatgg
     1621 gcggcgaagt ggaggatggc gtccacattg gcgtgccagt ccatggagcc gaggaaggcg
//
LOCUS       DAOMYJ010000501         1669 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Prosthecobacter sp. isolate AMR_MDS_5629
            NODE_28912_length_1669_cov_18.265180, whole genome shotgun
            sequence.
ACCESSION   DAOMYJ010000501 DAOMYJ010000000
VERSION     DAOMYJ010000501.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867850
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prosthecobacter sp. (wastewater metagenome)
  ORGANISM  Prosthecobacter sp.
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Verrucomicrobiaceae; Prosthecobacter.
REFERENCE   1  (bases 1 to 1669)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1669)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.30
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:34:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,742
            CDSs (total)                      :: 4,684
            Genes (coding)                    :: 4,676
            CDSs (with protein)               :: 4,676
            Genes (RNA)                       :: 58
            tRNAs                             :: 55
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1669
                     /organism="Prosthecobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28912_length_1669_cov_18.265180"
                     /isolate="AMR_MDS_5629"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1965333"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..996
                     /locus_tag="PLP58_23590"
     CDS             <1..996
                     /locus_tag="PLP58_23590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ11582.1"
                     /translation="SIIQAHAALKPAAVGAAAHPLPDEVFNRRWYLKEGAMPANPFGK
                     FDRVKMNPGAGNAALERPAGPIDPDITVLSVQDTKRNALALFANYSLHYVGGAPKGRM
                     SADYFGEFARLMPSRLRGGPDFVAMMSNGTSGDINNIPFTVTRPPREPFEQIRVVAQK
                     AADTAWFAHRKIEKHQTDVTLGMAQRKVTLQYRRPGAQEVAEAKAVLAVKDKAAIEAL
                     PRLAQNYARSTLNAHERPEATLDVTVQAIRIGDFAVCGIPFEVFVEIGLELKKRSPFP
                     QTMVIGLANGRHGYLPTPAHHKLGGYETWLGTNQVQEDASDILTANLLEMLAALK"
     gene            complement(1061..>1669)
                     /locus_tag="PLP58_23595"
     CDS             complement(1061..>1669)
                     /locus_tag="PLP58_23595"
                     /inference="COORDINATES: protein motif:HMM:TIGR02603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HRJ11583.1"
                     /translation="ATCGRLHGCESRQHTGLGAVLASAGQSDAALTAALGRILGVKNT
                     QPAYDPAWVEALAAEVKTAGDAEKGRGVYQNPLFGCTACHQIGGQGGIIGPELDAVGR
                     GVPLELLIEAVVWPGRQIKEGYVAANVTMKDGRRLQGYKFSEGGGELQLKELGTGTLL
                     RLALKDIQEHQESGSLMPEGLIVNMTREDLRDLVAYLAALGR"
BASE COUNT          278 a          674 c          477 g          240 t
ORIGIN      
        1 tccatcatcc aggcgcacgc ggctttgaaa cccgccgccg tcggtgccgc cgcgcacccc
       61 ctgccggacg aggtattcaa ccgccgctgg tatctcaaag agggcgccat gcccgcgaac
      121 cccttcggca aattcgaccg cgtgaagatg aaccccggcg ccggcaatgc cgccctggag
      181 cgccccgccg gccccattga cccggacatc accgtgctct ccgtgcagga cacgaagcgc
      241 aacgccctcg ccctcttcgc caactactcc ctccactacg tcggcggcgc gccgaagggg
      301 cggatgtccg ccgactactt cggcgagttc gcccgcctca tgccctcccg cctgcgcggc
      361 ggccccgatt tcgtcgccat gatgtcgaac ggcacctcgg gcgacatcaa caacatcccc
      421 ttcaccgtca cccgcccgcc tcgcgagccc ttcgagcaga tccgcgtcgt cgcgcaaaaa
      481 gccgccgaca ccgcctggtt cgcgcaccgg aaaatcgaga agcaccagac cgatgtcacc
      541 ctcggcatgg cgcagcgcaa agtgacgctc caataccgcc gccccggcgc gcaggaggtg
      601 gccgaggcca aggccgtgct cgctgtcaag gacaaggccg ccattgaggc cctgccgcgc
      661 ctggcgcaga actacgcgcg cagcaccctc aacgcccacg agcggcccga ggccacgctg
      721 gatgtcaccg tgcaggccat ccgcatcggc gacttcgccg tgtgcggcat ccccttcgag
      781 gtcttcgtcg agatcggcct cgaactcaaa aagcgcagcc ccttccccca gaccatggtc
      841 atcggcctcg ccaacggccg ccacggctac ctgcccaccc ccgcgcacca caagctcggc
      901 ggctacgaga cctggctcgg caccaaccag gtgcaggagg acgcctccga catcctcacc
      961 gcgaacctcc tggaaatgct cgccgcgctc aagtgagcgg caccgcgcct ggacacaagg
     1021 cgggccggct tgcccaccag ccgccagccg ccgcccccgg ctacctgccc agcgcggcga
     1081 ggtaggccac caagtcccgc agatcctccc gcgtcatgtt cacgatcagg ccctccggca
     1141 tcagcgagcc ggactcctgg tgctcctgga tgtccttcaa agccagccgc agcagcgtgc
     1201 cggtgcccag ctccttgagc tgcagctcgc cgccgccctc gctgaatttg tagccttgca
     1261 gccggcgtcc gtccttcatc gtcacattcg ccgccacata cccctccttg atctgccgcc
     1321 ccggccagac cacggcctcg atgagcagct ccagcggcac cccgcgcccc acggcgtcca
     1381 gctccgggcc gatgatgccg ccctgcccgc cgatttgatg gcaggccgtg cagccgaaca
     1441 agggattctg ataaacgccg cgccccttct ccgcgtcccc cgccgttttg acctcggcgg
     1501 ccaatgcctc gacccaggcg ggatcgtagg cgggctgggt gttcttcacg ccgaggatcc
     1561 tgccgagcgc ggcggtcaaa gccgcgtcac tctgccccgc ggaggccagc acggcaccga
     1621 ggcctgtgtg ctgtcgggac tcgcagccat gcagacgccc gcaggttgc
//
LOCUS       DAOMYJ010000502         1665 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Prosthecobacter sp. isolate AMR_MDS_5629
            NODE_29007_length_1665_cov_21.363975, whole genome shotgun
            sequence.
ACCESSION   DAOMYJ010000502 DAOMYJ010000000
VERSION     DAOMYJ010000502.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867850
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prosthecobacter sp. (wastewater metagenome)
  ORGANISM  Prosthecobacter sp.
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Verrucomicrobiaceae; Prosthecobacter.
REFERENCE   1  (bases 1 to 1665)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1665)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.30
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:34:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,742
            CDSs (total)                      :: 4,684
            Genes (coding)                    :: 4,676
            CDSs (with protein)               :: 4,676
            Genes (RNA)                       :: 58
            tRNAs                             :: 55
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1665
                     /organism="Prosthecobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29007_length_1665_cov_21.363975"
                     /isolate="AMR_MDS_5629"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1965333"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..472
                     /locus_tag="PLP58_23600"
     CDS             <1..472
                     /locus_tag="PLP58_23600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ11584.1"
                     /translation="REPQEDPPPVPRAIPVPDPIMSTPRPEKEAFSPFTRTSKTDGAI
                     QRPGENAVDAEATPLGRYMRQVTGAVERKWHLYVRLGKDSVNFGRVRFRFYVDPKGVP
                     QDLKILSDARDADPRMRELTLRAILDAQIPPIPPDLLPTLEDGRVKIEYEAIVY"
     gene            453..1166
                     /locus_tag="PLP58_23605"
     CDS             453..1166
                     /locus_tag="PLP58_23605"
                     /inference="COORDINATES: protein motif:HMM:NF013760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HRJ11585.1"
                     /translation="MKPLSIELPAPLLAAEEAPRGSLSLLMEFLATGGGTMFLIVACS
                     MVSVTVMISVWLRLRDRYVLPQSVRSQLRSLPRYADKGDIKPLQEFLEKDGSLLARLG
                     GKAISGTFASREECVETISARAKEELHHLERGVSVLEAMVTVAPLLGLMGTTIGLVGM
                     FTAFGGDAGPDTLAVAREIGVALRCTIAGLFVAVPSVVAHTYFVRRLDSIAVRMESIL
                     HETVQTFFAHFEVQRPPAR"
     gene            1185..1604
                     /locus_tag="PLP58_23610"
     CDS             1185..1604
                     /locus_tag="PLP58_23610"
                     /inference="COORDINATES: protein motif:HMM:NF014525.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HRJ11586.1"
                     /translation="MNLRPRRRPVPMIPIVSLVDVMVILLIFFVATTTFRNEKTQVKI
                     TLPESRTMGEPVPQAEVRKAITITREQELFLDGSPVQPDALADALKRLKAQSPAVRLE
                     LEADTGTALGVLMKVWDALRAAGYSVNDVPARIQRAP"
BASE COUNT          275 a          658 c          476 g          256 t
ORIGIN      
        1 gcgcgagccg caggaggacc cgccgcccgt gccgcgcgcc atccccgtgc cagaccccat
       61 catgagcacg ccgcggccgg agaaggaagc cttttcaccc ttcacccgca ccagtaagac
      121 cgacggcgcc atccagaggc ccggtgagaa cgccgtggac gccgaggcca ccccgcttgg
      181 ccgctacatg cgccaggtca ccggcgccgt ggagcgcaaa tggcacctct atgtgcgcct
      241 cggcaaagac tccgtgaact tcggccgcgt gcgcttccgc ttctatgtgg accccaaagg
      301 cgtgccgcag gacctcaaga tcctctccga cgcgcgcgat gccgacccca gaatgcggga
      361 attgaccttg cgcgccatcc tggatgcgca aatacctccg atccctcccg acctcctgcc
      421 gaccctcgaa gacggacggg tgaagatcga gtatgaagcc attgtctatt gaactcccag
      481 cccccctgct cgccgcggag gaagcgccgc gcggatcact ctccctcctc atggagttcc
      541 tcgccaccgg cggcggcacc atgttcctca tcgtcgcctg ctccatggtc tccgtcaccg
      601 tcatgatctc cgtctggctg cgtttgcgcg acaggtatgt gctgccgcag agcgtgcgca
      661 gccagctccg cagcctgccg cgttacgcgg acaaaggcga catcaaaccg ctgcaggagt
      721 tcctggagaa agacggctcc ctcctcgcca ggctcggcgg caaggccatc agcggcacct
      781 tcgcctcgcg cgaggagtgc gtggagacca tcagcgcccg cgccaaggag gagctgcacc
      841 acctcgagcg cggcgtctcc gtgctcgagg ccatggtcac cgtcgccccg ctgctcggcc
      901 tcatgggcac caccatcggc ctcgtcggca tgttcaccgc cttcggcggc gacgccgggc
      961 cggacaccct ggccgtcgcc cgcgagatcg gcgtcgccct gcgctgcacc atcgccggtc
     1021 tctttgtcgc cgtgccctcc gtcgtcgccc acacctactt cgtgcgccgg ctcgacagca
     1081 tcgccgtgcg catggagtcc atcctgcacg agaccgtgca gaccttcttc gcgcacttcg
     1141 aggtgcagcg cccccccgcc aggtgagccc cgcacccgtg agccatgaac ctgcgcccgc
     1201 gccgccgccc cgtccccatg atccccatcg tctcgctggt ggatgtcatg gtcatcttgc
     1261 tcatcttctt cgtcgccacc accaccttcc gcaatgaaaa gacccaggtg aaaatcaccc
     1321 tccccgagtc caggacgatg ggcgagcccg tcccccaggc cgaggtgcgc aaagccatca
     1381 ccatcaccag ggagcaggag cttttcctcg acggcagccc cgtccagcca gacgccctcg
     1441 ccgacgccct caagcgcctc aaagcgcaaa gccccgccgt caggctcgag ctcgaagccg
     1501 acaccggcac cgccctcggc gtgctcatga aagtctggga cgccctgcgc gccgccggct
     1561 actccgtcaa cgacgtgccc gcccgcatcc agcgcgcccc ctagccgtcg ccgcaaggcg
     1621 gcggtgtcat gccgccgcgg gagtcaaacc cgcccattgg caggc
//
LOCUS       DAOMYJ010000503         1661 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Prosthecobacter sp. isolate AMR_MDS_5629
            NODE_29112_length_1661_cov_14.577210, whole genome shotgun
            sequence.
ACCESSION   DAOMYJ010000503 DAOMYJ010000000
VERSION     DAOMYJ010000503.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867850
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prosthecobacter sp. (wastewater metagenome)
  ORGANISM  Prosthecobacter sp.
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Verrucomicrobiaceae; Prosthecobacter.
REFERENCE   1  (bases 1 to 1661)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1661)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.30
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:34:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,742
            CDSs (total)                      :: 4,684
            Genes (coding)                    :: 4,676
            CDSs (with protein)               :: 4,676
            Genes (RNA)                       :: 58
            tRNAs                             :: 55
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1661
                     /organism="Prosthecobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29112_length_1661_cov_14.577210"
                     /isolate="AMR_MDS_5629"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1965333"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            320..>1661
                     /locus_tag="PLP58_23615"
     CDS             320..>1661
                     /locus_tag="PLP58_23615"
                     /inference="COORDINATES: protein motif:HMM:NF016121.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding protein"
                     /protein_id="HRJ11587.1"
                     /translation="MKLPRSFSPVLLRVWRLGLLAAAVLLAREAERRREDVELASALT
                     AERVRDFFPKAENLEEPDARGRQAVRDAEGALLGHVTTTLPESRGIIGYSGPTHSLLA
                     FDPQGAVLGLRVLRSHDTPDHLTEVVAERRFFAQFRGKKPEELEGMELHAVTGATLTS
                     TAIAEGVLARLGAAAKVSLRFPEEITLEEVRRLEPAAEALKKHAGPAGGMEVLDGKGK
                     RIGIATRTSPVTDTLVGYKGPTDTLMLLDARGEKLRRIALRKSYDTARYVAYVTGDEY
                     FQNLFNERALEALAGMDFKAAKIEGVSGATETSWSVAEGLKRRAAHILEQRPAGWLRQ
                     VRWRWQDTGHAVMVLSALLMAFTRLRGVAWARHAHHALLVVYGGFIAGELLSQGLLAG
                     WAAHGTPWKSAPGLLLLAAVALLGPVFTGKHLYCHHLCAHGALQQLVARRTGVEA"
BASE COUNT          248 a          496 c          664 g          253 t
ORIGIN      
        1 catggggcgg cgctggccgg tctggaggcc agcaagggca tggtcgccca attcggccgc
       61 gccaagacgc tcggcgaggc ctgcatcggc ttccccgacg ccggcgcgct ctcggtgacg
      121 gtcatcctcg gggcgatgaa ggattacatc caggcctgag cggcgctgga gttcagcgtt
      181 ggagttcaag ctttagcttg ttcaatgaac tcccatagcg gagccaactc ccgtgtcgcc
      241 tgaaggcttc actccaacgg cagggtttgt acgcgcggat ttgcaaaaac cgttcacact
      301 ctccagcttg ggccaacgaa tgaaactgcc gcgctccttt tctcccgtcc tcctgcgcgt
      361 ctggcggctg gggctgctgg cggcggcggt gctgctggcg cgggaggcgg agcggcggcg
      421 ggaggatgtg gagctggcct cggcgctgac ggcggagcgg gtgcgggatt tttttcccaa
      481 ggcggaaaat ctggaggagc cggacgcgcg cggacgccag gcggtgcggg acgcggaggg
      541 ggcgctgctg gggcatgtga cgacgacgct gccggagtcg cgcggcatca tcggctactc
      601 ggggccgacg cactcgctgc tggcctttga tccgcaaggc gcggtgctgg gcctgcgcgt
      661 gctgcgcagc cacgacacgc cggaccacct gacggaggtg gtggcggagc ggaggttctt
      721 cgcgcaattc cggggcaaga aaccggagga gctggaaggc atggagctgc acgcggtgac
      781 gggcgccacg ctgacgagca cggccatcgc ggagggtgtg ctggcgcggc tgggcgcggc
      841 ggcgaaggtg tcgctgcgct tcccggagga gatcacgctg gaggaggtgc ggcggctgga
      901 gcctgcggcg gaggcactga aaaaacacgc ggggcccgcg ggcggcatgg aggtgctgga
      961 cgggaaggga aagcgcatcg gcatcgccac gcgcacctcg ccggtgacgg acacgctggt
     1021 cggctacaaa gggccgacgg acacgctgat gctgctggac gcgcgcgggg agaagctgcg
     1081 ccggatcgcg ctgcgcaaga gctacgacac ggcgcgctat gtggcctatg tcacggggga
     1141 cgagtatttt caaaatctct tcaacgagcg cgcgctggag gcgctggcgg gcatggactt
     1201 caaggcggcg aagatcgaag gcgtgtccgg cgccacggag acgagctggt cggtggcgga
     1261 ggggctgaag cggcgcgcgg cgcacatcct ggagcagcgg cccgccggct ggctgcggca
     1321 ggtgcgctgg cgctggcagg acacgggcca cgcggtgatg gtgctctcgg ctttgctgat
     1381 ggccttcacg cgcctgcgcg gggtggcctg ggcgcggcac gcgcaccacg cgctgctggt
     1441 ggtgtacggc ggcttcatcg ccggggagct gctctcgcaa gggctgctgg ccggatgggc
     1501 ggcgcacggc acgccgtgga aaagcgcgcc gggtctgctg ctgctggcgg cggtggcgct
     1561 gctggggccg gtcttcaccg ggaagcactt gtactgccat cacctgtgcg cgcacggggc
     1621 cttgcagcag ctcgtggcgc ggcgcaccgg cgtggaggcg c
//
LOCUS       DAOMYJ010000504         1648 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Prosthecobacter sp. isolate AMR_MDS_5629
            NODE_29456_length_1648_cov_12.592593, whole genome shotgun
            sequence.
ACCESSION   DAOMYJ010000504 DAOMYJ010000000
VERSION     DAOMYJ010000504.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867850
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prosthecobacter sp. (wastewater metagenome)
  ORGANISM  Prosthecobacter sp.
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Verrucomicrobiaceae; Prosthecobacter.
REFERENCE   1  (bases 1 to 1648)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1648)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.30
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:34:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,742
            CDSs (total)                      :: 4,684
            Genes (coding)                    :: 4,676
            CDSs (with protein)               :: 4,676
            Genes (RNA)                       :: 58
            tRNAs                             :: 55
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1648
                     /organism="Prosthecobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29456_length_1648_cov_12.592593"
                     /isolate="AMR_MDS_5629"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1965333"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            169..537
                     /locus_tag="PLP58_23620"
     CDS             169..537
                     /locus_tag="PLP58_23620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ11588.1"
                     /translation="MSLQARAGNECAAMRARLASMRVIIGELEDMLPAARELLASLRV
                     EMCCLATDINALDTTCGALRMTGQQPPLQPQDPVESTAHKAMLRSLHRAGDHTLDPED
                     DEGNSKFEIRNSNFGEGGGR"
     gene            534..1211
                     /locus_tag="PLP58_23625"
     CDS             534..1211
                     /locus_tag="PLP58_23625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ11589.1"
                     /translation="MIPGEGKTTGELFEAARALLTELETELGRRRRRERACTALTDLH
                     LALQALARRVGGVEWAEATVCHEDEDELEGAEPAPMELGMRELMAAPGDELEAQAAAA
                     EGVEVWELRLRYFRLFLDFQVAGCRTFEEVALKCLALVRRLRPEMLERFGLSMAEVGR
                     KTGRSRANIHAFEKRMLEELLKGAGARGFHGLGGQRGAETRERCRRAQQGNQNRRNGE
                     ARKRAEG"
     gene            1256..>1648
                     /locus_tag="PLP58_23630"
     CDS             1256..>1648
                     /locus_tag="PLP58_23630"
                     /inference="COORDINATES: protein motif:HMM:NF014273.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB N-terminal domain-containing protein"
                     /protein_id="HRJ11590.1"
                     /translation="MNPAKAEIVFYPAELIDPSPYQVRRKFDEAALEELAESIKARGI
                     VQPLTARVSPRDAARLELRVVPVEVDELVERVVASVRWMVGKKRLTIDVAMTPHLGLS
                     SDRGRIAQILVNLLANAVKFTDEGGAVTL"
BASE COUNT          288 a          412 c          688 g          260 t
ORIGIN      
        1 tcggccttcg cgagttgggc gcagtagtac gccggcaggg cagaggagtt gccgccgcgc
       61 gggtgtgctc tcggggtggt cggacggggt gtggtgggag cggcggcggg gggattttcg
      121 ggagcggctg ggtgccgatt ttcccgagca caacgaaagg aggctgccat gagtctgcag
      181 gcgcgggcgg gcaatgaatg cgcggcgatg cgggcgcggc tggcgtccat gcgtgtgatc
      241 attggcgagc tggaggatat gctgccggcg gcgcgggagc tgctggcgag cctgcgtgtg
      301 gaaatgtgct gtctggcgac ggacatcaac gcgctggaca cgacatgcgg cgcgttgcgc
      361 atgacggggc agcagccgcc gctgcagccg caggatccgg tggagagcac ggcgcacaag
      421 gcgatgctac ggtcgctgca cagagccggg gatcacacgc tggatccaga ggatgatgag
      481 gggaattcaa aattcgaaat tcgaaattcg aattttgggg aagggggtgg gcggtgattc
      541 cgggcgaggg caaaacgacg ggggagctgt tcgaggcggc gcgggctttg ctgaccgaac
      601 tggagaccga gttgggcagg cggcgccggc gggagagggc ctgcacggct ctgacggatc
      661 tgcatctggc gttgcaggcg ctggcgcggc gggtgggggg tgtggagtgg gctgaggcga
      721 cggtgtgcca tgaggatgag gatgagctgg agggcgcgga gcctgcgccg atggagctgg
      781 gcatgcgcga gctgatggcg gcgccgggcg atgagctgga ggcgcaggcg gcggcggcgg
      841 agggggtgga ggtgtgggaa ctgcggctga gatacttccg gttattcctg gacttccagg
      901 tggcgggctg ccgaaccttt gaggaggtgg cgctgaaatg cctggctctg gtgcgcaggc
      961 tgaggccgga gatgctggag cgcttcggtc tgagcatggc ggaggtgggg cgcaagacgg
     1021 ggcgcagccg ggcgaacatc cacgcctttg agaagcgcat gctcgaagag ctgctgaaag
     1081 gcgcgggcgc gcgcggtttc cacggcctgg gcgggcagcg gggggctgag acgcgggagc
     1141 gctgccggcg ggcgcagcag ggcaatcaaa accgccggaa cggggaggcg cggaaacgcg
     1201 cggaggggtg accattttcc gaaccaacaa accaacaaac caaacatctg aagttatgaa
     1261 ccctgcaaaa gctgaaatcg ttttttatcc ggctgaattg attgatccga gcccttacca
     1321 ggtgcggcgg aaatttgatg aggcggctct cgaagagttg gcggagagca tcaaggcgcg
     1381 cgggatcgtg cagcctctga cggcgcgtgt gtcgccgcga gacgcggcgc ggctggagct
     1441 gcgcgtggtg ccggtggagg tcgacgagct ggtggagcgg gtggtggcgt cggtgcgctg
     1501 gatggtcggg aagaagcgcc tgacgatcga cgtcgcgatg accccacacc tcgggctctc
     1561 gagcgaccgc gggcgcatcg cgcagatcct cgtgaacctg ctcgcgaacg cggtgaagtt
     1621 caccgacgaa ggcggcgcgg tcacgctt
//
LOCUS       DAOMYJ010000505         1590 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Prosthecobacter sp. isolate AMR_MDS_5629
            NODE_31091_length_1590_cov_21.244951, whole genome shotgun
            sequence.
ACCESSION   DAOMYJ010000505 DAOMYJ010000000
VERSION     DAOMYJ010000505.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867850
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prosthecobacter sp. (wastewater metagenome)
  ORGANISM  Prosthecobacter sp.
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Verrucomicrobiaceae; Prosthecobacter.
REFERENCE   1  (bases 1 to 1590)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1590)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.30
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:34:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,742
            CDSs (total)                      :: 4,684
            Genes (coding)                    :: 4,676
            CDSs (with protein)               :: 4,676
            Genes (RNA)                       :: 58
            tRNAs                             :: 55
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1590
                     /organism="Prosthecobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31091_length_1590_cov_21.244951"
                     /isolate="AMR_MDS_5629"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1965333"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..507
                     /locus_tag="PLP58_23635"
     CDS             <1..507
                     /locus_tag="PLP58_23635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009957951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HRJ11591.1"
                     /translation="VAQELGVADVRAGLHPEDKVAAIREFTRAGKKTAMLGDGVNDAP
                     SLAAADVSIAMGARGSDAALEQADIVLMQDRIEKLLSARHISRRARAIIRQNLAISLG
                     SVVVMAVASLFGIVPLTLGVLTHEGSTVVVCLNSLRLLFVREPGMGQGMGHRRPACAG
                     NRQDSRTP"
     gene            complement(508..1113)
                     /gene="recR"
                     /locus_tag="PLP58_23640"
     CDS             complement(508..1113)
                     /gene="recR"
                     /locus_tag="PLP58_23640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009963442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination mediator RecR"
                     /protein_id="HRJ11592.1"
                     /translation="MPALDYPPPLQRMIAQVKALPGLGPRSAERLVLWLLQSGGRIEP
                     LVDALENLHHRVHACPECGFYIEQGHDCSLCHSPKRDARLICVVEQAADVLRLERSGA
                     FTGLYHVLGGKLSPLDNVTPEDLRIQPLLDRLRAAAVQEVILAMGSDVEGEATASYLS
                     DLLRAEGVAVSRLAQGMPAGGGLDHVDELTLYQALHGRRRL"
     gene            1218..>1590
                     /locus_tag="PLP58_23645"
     CDS             1218..>1590
                     /locus_tag="PLP58_23645"
                     /inference="COORDINATES: protein motif:HMM:NF012610.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HRJ11593.1"
                     /translation="MKALIQRQSLITQAAALLRGELERGTWREFLPGERQLAVQMGIS
                     RPTLRGALECLAAEGWLTRVHGRPTRITRPASTTPARRTQVILLSPVPLREMPPLMVC
                     WVDELREKLAAAEHALELLTLP"
BASE COUNT          246 a          452 c          655 g          237 t
ORIGIN      
        1 gtggcgcagg agctgggcgt ggcggatgtg cgcgcgggcc tgcacccgga ggacaaggtg
       61 gcggcgatcc gggagttcac gcgcgcgggc aagaagacgg cgatgctggg ggacggtgtg
      121 aatgacgcgc cgagcctggc ggcggcggat gtgagcatcg ccatgggtgc gcgcggcagt
      181 gacgcggcgc tggagcaggc ggacattgtg ctgatgcagg accgcatcga gaagctgctc
      241 tcggcgcggc acatcagccg gcgggcgcgt gcgatcatcc ggcagaactt ggcgatctcc
      301 ctcggctcgg tggtggtgat ggcggtggcc tcgctcttcg gcatcgtgcc gctgacgctg
      361 ggcgtgctga cgcacgaggg cagcacggtg gtggtgtgcc tgaacagcct gcggctgctg
      421 tttgtgcggg agcctggcat ggggcaaggt atggggcaca ggcgtcccgc ctgtgccggg
      481 aacaggcagg attcccgtac cccatgatca caaccgccgc cggccgtgca gggcctggta
      541 aagcgtgagc tcgtccacat gatcaagccc gccgccggcg ggcatgccct gggcgaggcg
      601 ggagacagcg acgccttcgg cgcggaggag gtcgctcagg tagctggcgg tggcctcgcc
      661 ttcgacatcg ctgcccatgg cgaggatgac ctcctgcacg gcggcggcgc gcagacggtc
      721 gagcaggggc tggatgcgca ggtcctcggg ggtgacgttg tcgagcgggg agagcttgcc
      781 tccgagcaca tggtagaggc cggtgaaggc gccggagcgc tcaaggcgga ggacatcggc
      841 ggcctgctcg acgacgcaga taagacgggc atcccgcttc gggctgtggc agagggagca
      901 gtcgtggcct tgttcgatgt agaaaccgca ctcggggcag gcgtggacgc ggtggtggag
      961 gttttccagc gcgtccacga gcggctcgat gcggccgccg ctttgcagga gccagagcac
     1021 gaggcgctcg gcgctgcggg gcccgaggcc ggggagggcc ttgacctggg cgatcatgcg
     1081 ttgcaggggc ggcgggtagt cgagggcggg catgggcggg gcaggggatg gaaggagcca
     1141 agattccgcg ggatggcaag tcagggtgtg cattgacctg ggtgcggtgt ctgctttgat
     1201 ggacggcaac gccaactgtg aaagccctca tccaacgcca gtcgctgatc acccaggccg
     1261 ccgcgctgct gcggggcgag ctggagcgcg ggacatggag ggagtttttg ccgggggaaa
     1321 gacagctcgc ggtgcagatg ggcatcagcc gcccgacatt gcgcggagca ttggaatgcc
     1381 tggcggcgga gggctggctg acacgcgtgc atggcaggcc gacgcggatt acccgccctg
     1441 cgagcacgac gccggcgcgg cgcacgcagg tgatcttgct cagccccgtg ccgctgcggg
     1501 agatgccgcc gctgatggtg tgctgggtgg acgaactgcg ggagaagctg gcggcggcgg
     1561 agcacgcgct ggagctgctg acgctgccgg
//
LOCUS       DAOMYJ010000506         1584 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Prosthecobacter sp. isolate AMR_MDS_5629
            NODE_31286_length_1584_cov_15.712884, whole genome shotgun
            sequence.
ACCESSION   DAOMYJ010000506 DAOMYJ010000000
VERSION     DAOMYJ010000506.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867850
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prosthecobacter sp. (wastewater metagenome)
  ORGANISM  Prosthecobacter sp.
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Verrucomicrobiaceae; Prosthecobacter.
REFERENCE   1  (bases 1 to 1584)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1584)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.30
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:34:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,742
            CDSs (total)                      :: 4,684
            Genes (coding)                    :: 4,676
            CDSs (with protein)               :: 4,676
            Genes (RNA)                       :: 58
            tRNAs                             :: 55
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1584
                     /organism="Prosthecobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31286_length_1584_cov_15.712884"
                     /isolate="AMR_MDS_5629"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1965333"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..786)
                     /locus_tag="PLP58_23650"
     CDS             complement(<1..786)
                     /locus_tag="PLP58_23650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ11594.1"
                     /translation="MKWNDWQGILRWRAMEEADADGALLSLERRQEATARTRGGLSSW
                     DAQGGALNEREAAFLKKRAEWLEREVIGWGSPVARVMERLRAPHGRWSWALCGWAAAF
                     AAGWGLSGLGQEAEFNLLALPLVGLLLWNAVVMIAALVLDLHPAGPAREPEGLLAWLA
                     RLVMPRSEERAAEGETVTGLTADQRFALLANLPALERLCRRLRAWLHIAAAVLALGGV
                     CGMYARGWSKEYRAVWESTLLDEAKAVGFFDALFSPAAAVWRLE"
     gene            complement(799..1176)
                     /locus_tag="PLP58_23655"
     CDS             complement(799..1176)
                     /locus_tag="PLP58_23655"
                     /inference="COORDINATES: protein motif:HMM:NF017103.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF721 domain-containing protein"
                     /protein_id="HRJ11595.1"
                     /translation="MSTRHATAGQRRRHALVSAWRGVEDGPVLNLPTVSVADLVGKVV
                     AQAGLSERMRLEEVLAAWREIVGDFLFQHSRPDSIQRGVLMVRVLQPAVHHALTLERP
                     RILKRLQERLKNAGIKDVRLKHG"
     gene            1311..>1584
                     /locus_tag="PLP58_23660"
     CDS             1311..>1584
                     /locus_tag="PLP58_23660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="prenyltransferase"
                     /protein_id="HRJ11596.1"
                     /translation="MITDWIQAARPKTLGAAVAPVAAGSVLGWKLGGAWNWTLFACTL
                     GSCIALQVATNWFNDALDFLKGADTRARLGPRRITAAGVVPPGAVLR"
BASE COUNT          260 a          622 c          476 g          226 t
ORIGIN      
        1 ctccagcctc cacaccgccg ccgccggact gaacagcgcg tcgaaaaacc ccaccgcctt
       61 cgcctcgtcc aaaagcgtgc tctcccacac cgcgcggtat tccttcgacc agccgcgcgc
      121 atacatcccg cacacgccgc ccagcgccag caccgccgcc gcgatgtgca gccatgcgcg
      181 caaccgcctg cacaggcgct ccagcgctgg caggtttgcc agcagggcaa aacgctggtc
      241 cgctgtcagg cccgtcacag tctcgccctc cgcagcgcgc tcctcggagc gcggcatgac
      301 cagccgcgcc agccatgcca ggagaccctc cggctcgcgc gccggccccg caggatgcag
      361 gtccagcacc agtgccgcga tcatcaccac cgcgttccac aggagcagcc ccaccagagg
      421 cagcgccagg aggttgaact ccgcctcctg ccccaggccg gaaagccccc agccggccgc
      481 gaaggccgcc gcccagccgc acaaggccca ggaccagcgg ccatgcggcg cgcgcaggcg
      541 ctccatcacc cgcgccaccg gactgcccca gccgatcacc tcccgttcca gccactccgc
      601 gcgttttttc aggaatgcag cctccctctc gttcagggcg cctccctgcg cgtcccagga
      661 ggacaggccg ccgcgagtgc gcgccgtcgc ctcctggcgg cgctcaaggc tcagcagcgc
      721 gccatcggcg tccgcctcct ccatcgcgcg ccagcgcagg atgccctgcc agtcattcca
      781 tttcatgccg ccgggtgttc aaccatgttt gagccttacg tccttgatgc cggcattttt
      841 cagccgctcc tgcagcctct tcaaaatgcg cggacgctcc agcgtcagcg cgtgatgcac
      901 ggcaggctgg agcacgcgca ccatcagcac gccgcgctgg atggagtccg gcctggagtg
      961 ttgaaaaagg aaatccccca cgatctcccg ccacgccgcc agcacctctt ccagccgcat
     1021 ccgctccgac agccccgcct gcgccaccac cttgcccacc aggtccgcca cgctcaccgt
     1081 tggcaggttc agcacaggcc cgtcctccac gccccgccat gcggagacga gagcgtggcg
     1141 gcggcgttgc ccggcggtgg catggcgcgt tgacatgcgg tcaagatgac ggcgccggag
     1201 aaggaatgcg agaaggaaaa tgagattcaa gaccgccgcc gcaatttctc cccgtgggat
     1261 gcggcggcgc gcttcttgac gcggacgggg gagagccgca aaactgcgtg atgatcaccg
     1321 actggattca ggccgcgcgc cccaagactc tcggcgccgc cgtggcgccc gtggccgccg
     1381 gttccgtgct tggctggaag ctgggcggag catggaactg gacgctcttt gcctgcacgc
     1441 tgggaagctg catcgcgctc caggtggcca cgaactggtt caacgacgcg cttgatttcc
     1501 tcaaaggcgc ggacacccgc gcgcgcctcg gtccgcggcg aatcacggcg gccggtgtgg
     1561 tcccgcccgg cgccgtgctt cgcc
//
LOCUS       DAOMYJ010000507         1567 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Prosthecobacter sp. isolate AMR_MDS_5629
            NODE_31820_length_1567_cov_15.460979, whole genome shotgun
            sequence.
ACCESSION   DAOMYJ010000507 DAOMYJ010000000
VERSION     DAOMYJ010000507.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867850
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prosthecobacter sp. (wastewater metagenome)
  ORGANISM  Prosthecobacter sp.
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Verrucomicrobiaceae; Prosthecobacter.
REFERENCE   1  (bases 1 to 1567)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1567)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.30
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:34:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,742
            CDSs (total)                      :: 4,684
            Genes (coding)                    :: 4,676
            CDSs (with protein)               :: 4,676
            Genes (RNA)                       :: 58
            tRNAs                             :: 55
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1567
                     /organism="Prosthecobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31820_length_1567_cov_15.460979"
                     /isolate="AMR_MDS_5629"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1965333"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(148..>1567)
                     /locus_tag="PLP58_23665"
     CDS             complement(148..>1567)
                     /locus_tag="PLP58_23665"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: protein motif:HMM:NF013670.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HRJ11597.1"
                     /translation="PMHPDRPASRRRPRGLIAALTASLLATAAPLHADVTTDTEKKGF
                     FGRIFGGGKKEEPAPPPAPEKPETPSTPGPKPTVTTKSKAGSSTKPATSTSKTPPPPA
                     KKSEPAKPTPAKPVAAAEKKSAPPPAPPKTAAVKPAGDGEFAQDRGELKPPPPSTPLP
                     PAARQSGGWQIVNINGRDYVTGESLHQFYRFSSYKVEGTHVWLRSNNLIIKATIGSQE
                     MLINNIKFILSYPVQEYSGRALFSRIDLSKLIDPVLRPSHITDAVPFDTVVVDAGHGG
                     HDAGARGIYGYEKDFALQMARSVRDALQRKGFKVIMTRDSDTFITLGGRVAVANNTPN
                     CIFISLHFNSGGSEAANGIETFALTPQGSSASLERGGGYNANGLTGNSHDSANIALAT
                     AVHAMVISKFRFVDRGIKRAQWSVLSGCKKPGILFEGGFVTNPNECRLIASDTYRQQV
                     SASIADAVVNYRTALKGAMTRR"
BASE COUNT          223 a          415 c          626 g          303 t
ORIGIN      
        1 gagaaggaag agggcgcggg acttcatggc ggcggggact ttatacgcgg aggcgctggg
       61 cgggctgtca gcgtgcaacc tggaaggttg caccgccttg cgcgggcagg atgcccggtt
      121 cacacgagac aggcggggac gcctgtccta ccggcgggtc atggcgcctt tgagggcggt
      181 gcggtagttc accacggcgt cggcgatgga ggcggagacc tgctggcggt aggtgtcgga
      241 ggcgatgagg cggcactcgt tggggttggt gacaaagccg ccctcgaaga gaatgccggg
      301 ttttttgcag ccggagagca cgctccactg ggcgcgcttg atgccgcggt ccacgaagcg
      361 gaacttgctg atgaccatgg cgtggacggc ggtggcaagg gcgatgttgg cggagtcgtg
      421 ggagttgccg gtgaggccgt tggcgttgta gccgccgccg cgctcaaggc tggcggagct
      481 gccctgcggg gtgagggcga aggtctcgat gccgttggcg gcctcgctgc cgccggagtt
      541 gaagtggagg ctgatgaaga tgcagttggg cgtgttgttc gccacggcca cgcgcccgcc
      601 gagggtgatg aaagtgtcgc tgtcgcgggt catgatgact ttgaagccct tgcgctggag
      661 cgcgtcgcgc acggaacgcg ccatttgcag ggcgaagtct ttttcataac catagatgcc
      721 gcgcgcgccg gcgtcgtgac cgccgtggcc cgcgtccacg acgacggtgt cgaaggggac
      781 ggcgtcggtg atgtgggagg ggcggagcac ggggtcaatg agcttgctga ggtcaatgcg
      841 cgagaagagc gcgcgcccgg agtattcctg caccgggtag ctgaggatga acttgatgtt
      901 gttgatcagc atctcctggc tgccgatggt ggccttgatg atgaggttgt tcgagcgcag
      961 ccagacatgc gttccctcca ccttgtagga gctgaagcgg taaaactggt ggagactctc
     1021 gccggtgacg tagtcgcgcc cgttgatgtt gacgatctgc cagccgccgg actggcgcgc
     1081 ggcgggcggc agcggggtgg acgggggcgg cggcttcagt tcgccgcgat cctgggcgaa
     1141 ctcgccatcg cccgcaggct tcacggcggc ggttttcggc ggggcgggag gcggcgcgga
     1201 ttttttctcg gctgccgcca cgggcttggc cggggtgggt ttggccggct cgctcttttt
     1261 cgcaggcgga ggcggggtct tggaagtgct ggtggccggc tttgtggatg agccggcttt
     1321 cgacttggtc gtcaccgtgg gtttggggcc gggtgtggag ggtgtctccg gcttttcagg
     1381 cgctggcggc ggcgcgggct cctctttttt gccgccgcca aagatgcggc caaaaaaacc
     1441 cttcttttcc gtgtccgtgg tgacatccgc gtggagcggc gcggcggtcg ccagcaacga
     1501 ggcggtcagt gcggcaatca agccgcgcgg gcggcgtcgg gaagctggtc tgtcagggtg
     1561 catgggt
//
LOCUS       DAOMYJ010000508         1560 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Prosthecobacter sp. isolate AMR_MDS_5629
            NODE_32023_length_1560_cov_15.928239, whole genome shotgun
            sequence.
ACCESSION   DAOMYJ010000508 DAOMYJ010000000
VERSION     DAOMYJ010000508.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867850
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prosthecobacter sp. (wastewater metagenome)
  ORGANISM  Prosthecobacter sp.
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Verrucomicrobiaceae; Prosthecobacter.
REFERENCE   1  (bases 1 to 1560)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1560)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.30
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:34:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,742
            CDSs (total)                      :: 4,684
            Genes (coding)                    :: 4,676
            CDSs (with protein)               :: 4,676
            Genes (RNA)                       :: 58
            tRNAs                             :: 55
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1560
                     /organism="Prosthecobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32023_length_1560_cov_15.928239"
                     /isolate="AMR_MDS_5629"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1965333"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..294)
                     /locus_tag="PLP58_23670"
     CDS             complement(<1..294)
                     /locus_tag="PLP58_23670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ11598.1"
                     /translation="MSLDTSSLHGTEDPEMRHRLPRWYSRLLGLLVLAVIVHEVGFTG
                     GVTPLMRWATALLVLLFAAEHAAGLWRAARPADYARLQREAQALTPGGRGAAPE"
     gene            complement(310..798)
                     /locus_tag="PLP58_23675"
     CDS             complement(310..798)
                     /locus_tag="PLP58_23675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ11599.1"
                     /translation="MPQLTPDQLAHVETWAAGGATLNQIQDRLKNDFGITLTYLEARL
                     LMTEIGVRLKDKERPAEKKPDPEPEPPGRDEDAAAPPSEDPSAAPAGGSLTLRVDQIA
                     VPGTMASGKVTFSDGVTATWYVDQMGRLGLGGAPQGYQPPPADVPAFQRQLDRALMQA
                     GF"
     gene            complement(802..>1560)
                     /locus_tag="PLP58_23680"
     CDS             complement(802..>1560)
                     /locus_tag="PLP58_23680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ11600.1"
                     /translation="QRPAIPEELLGDEHVREEFGVNQFTTPSIRKLFEMLDGLGKLSY
                     DALKRPITRQTPSDRVLVSMGLGTLIADGFLIVQCEKVEAMEDVGRALIKYAKVLGAG
                     ARMNRHTQSLFEHSIEGEWDKLRTELAATQADVEAEMVQLRDVDIAHLVSLGGWLRAF
                     EIATRSVADHYTPEKTTRLLRAEIASYYQMSLDHLNPRLASHESLLKIRDGLKEIAPL
                     MDKPEGATLTEAEVGALHTRAAALAGIVTGAMKL"
BASE COUNT          216 a          465 c          610 g          269 t
ORIGIN      
        1 ttctggcgca gcaccgcggc cccccggggt gagcgcctgc gcctcgcgct gcagccgcgc
       61 gtagtccgcc gggcgcgcgg cgcgccaaag gccggcggcg tgctcggcgg cgaagagcag
      121 cacaagcagg gcggtggccc accgcatgag cggagtcacg ccgccggtga agccgacctc
      181 atggacgatg acggccagca cgagcaggcc aagcagccgg gaataccagc gcggcagcct
      241 gtgccgcatt tcagggtctt cggtgccgtg gaggctggag gtgtcgagtg acacaaaaag
      301 ggggggcgct cagaagcccg cctgcatgag cgcgcggtcg agctggcgct ggaaggcggg
      361 cacgtcggcc gggggcggct ggtaaccctg cggggcgccg ccgaggccga ggcggcccat
      421 ttgatccaca taccaggtgg cggtgacgcc gtcgctgaaa gtgaccttgc cgctggccat
      481 cgtgccgggc acggcgatct ggtccacgcg cagggtcaaa gaaccgccgg ccggcgcggc
      541 tgacgggtct tcggagggcg gcgcggcagc gtcttcatcc ctgccgggcg gctccggctc
      601 cgggtcgggc tttttctcgg cgggacgctc cttgtctttg aggcggacgc cgatttcggt
      661 catgagcagg cgcgcctcga ggtaggtgag ggtgatgccg aagtcgtttt tgaggcggtc
      721 ctggatttgg ttgagcgtgg ctcctccggc ggcccaggtt tcgacatggg cgagttgatc
      781 aggggtcagt tgcggcatag gtcagagttt catggcgccg gtgacgatgc cggcgagggc
      841 ggcggcgcgg gtgtggagcg cgcccacctc cgcctcggtg agggtggcgc cttcaggctt
      901 gtccatgagg ggggcgattt ctttgagacc gtcgcggatt ttcagcaggg actcgtggct
      961 ggcgaggcgg gggttcagat ggtcgaggct catctggtag tagctggcga tctcggcgcg
     1021 caggaggcgg gtggttttct ccggtgtgta gtggtcggcc acgctgcggg tggcgatctc
     1081 gaaggcgcgc agccagccgc cgaggctgac gaggtgggcg atgtccacat cgcggagctg
     1141 gaccatctcg gcctcgacat cggcctgggt ggcggcgagc tcggtgcgca gcttgtccca
     1201 ctcgccctcg atgctgtgct cgaagaggct ctgggtgtgg cggttcatgc gcgcgccggc
     1261 gccgagcacc ttggcgtatt tgatcagggc gcggcccacg tcctccatgg cctccacttt
     1321 ctcgcattgc acgatgagga agccgtcggc gatgagggtg cccaggccca tggagacgag
     1381 cacgcggtcg gagggcgtct ggcgtgtgat ggggcgcttg agcgcgtcat aggagagctt
     1441 gccgaggccg tcgagcatct cgaagagctt gcggatgctg ggggtggtga actggttcac
     1501 gccgaactcc tcgcgcacat gctcgtcccc cagcagctcc tccgggatgg ccgggcgctg
//
LOCUS       DAOMYJ010000509         1548 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Prosthecobacter sp. isolate AMR_MDS_5629
            NODE_32378_length_1548_cov_15.951105, whole genome shotgun
            sequence.
ACCESSION   DAOMYJ010000509 DAOMYJ010000000
VERSION     DAOMYJ010000509.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867850
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prosthecobacter sp. (wastewater metagenome)
  ORGANISM  Prosthecobacter sp.
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Verrucomicrobiaceae; Prosthecobacter.
REFERENCE   1  (bases 1 to 1548)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1548)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.30
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:34:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,742
            CDSs (total)                      :: 4,684
            Genes (coding)                    :: 4,676
            CDSs (with protein)               :: 4,676
            Genes (RNA)                       :: 58
            tRNAs                             :: 55
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1548
                     /organism="Prosthecobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32378_length_1548_cov_15.951105"
                     /isolate="AMR_MDS_5629"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1965333"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..499)
                     /locus_tag="PLP58_23685"
     CDS             complement(<1..499)
                     /locus_tag="PLP58_23685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRJ11601.1"
                     /translation="MIETLKQGGSRGRDAALARLCTEYYPQVLNLAREQGLDAGTARA
                     MTRDFLTGMAGEGRWERFDPAEGRTLGEWVMECFQAFLTGKRGSAAGHDGAGRNGVKP
                     PAPRLPEPERARRNDDLPPVKPGPDFDLALAREIWSAARVYLVRKHQSSAHAELVEEL
                     APHVLA"
     gene            699..1511
                     /locus_tag="PLP58_23690"
     CDS             699..1511
                     /locus_tag="PLP58_23690"
                     /inference="COORDINATES: protein motif:HMM:NF013429.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HRJ11602.1"
                     /translation="MRFAICNETWPDTPFEQVCADAAGAGYHALELAPFTLKDDPRQL
                     TEADALRLSRIASDHGLDILGLHWLLTKPAWFHITTPDALLRRDTAAFGRHLARFCAM
                     TGGRIMVWGSPKARNTLPEWQYDDAFQRAADTVRLVAEEAGKYGVVIAMEPLGRKETN
                     FLNTAAETIRFCQLVDHPACRLHLDVKAMSDEATPIPDIIRASKDWFVHFHANDPNLL
                     GPGMGEVKYEPVIAALRDTAYTGYLSVEVFDYSPGPARIARESIRYLKQVLA"
BASE COUNT          246 a          675 c          395 g          232 t
ORIGIN      
        1 ccgccagcac atgcggcgcc agctcctcca ccagctccgc atgcgcgctc gactggtgct
       61 tgcgcaccag gtaaacccgc gccgcgctcc agatttcccg cgccagcgcc agatcgaagt
      121 ccggccccgg cttcacaggc ggcagatcat cattcctccg ggcgcgctcc ggctccggca
      181 gccttggggc gggcggtttc acgccgttgc gccccgcgcc gtcatgcccc gccgcgctcc
      241 cgcgtttgcc cgtcaggaaa gcctggaagc actccatcac ccactcgccc agggtgcgcc
      301 cctccgcggg gtcaaagcgc tcccagcgcc cctcgcccgc catgccggtg aggaaatccc
      361 gcgtcatcgc cctggccgtc cccgcgtcca gcccctgctc ccgcgccagg ttcagcacct
      421 gcggataata ttccgtgcaa aggcgcgcca gcgccgcgtc gcgccccctc gagccgccct
      481 gcttgagcgt ctcgatcatg tccggggatg gcgcgcggga ggaggaatcg gaggaggaat
      541 gggttctcac aaccggcggt actccctttt tcattttccg ttacgcattt tgccaaactt
      601 ttttccagcc gcccgcgtcc cgcccctccg cagctcatcc cggcccgcgc caaacccccg
      661 gttgactccg cactcccgcc gctcatgtct aggcccctat gcgcttcgcc atctgcaacg
      721 aaacctggcc cgacaccccc tttgagcaag tctgcgccga cgccgccggc gccggctacc
      781 acgccctcga gctcgccccc ttcaccctca aggacgaccc gcgccagctc accgaggccg
      841 acgcgctccg cctcagccgc atcgcctccg accacggcct cgacatcctc ggcctccact
      901 ggctgctcac caagcccgcc tggttccaca tcaccactcc tgacgccctc ctgcgccgcg
      961 acaccgccgc cttcggccgc cacctcgccc gcttctgcgc catgaccggc ggccgcatca
     1021 tggtctgggg cagccccaag gccaggaaca ccctgcccga atggcaatac gacgacgcct
     1081 tccagcgcgc cgccgacacc gtccgcctcg tcgccgagga agccggcaaa tacggcgtcg
     1141 tcatcgccat ggagcccctc ggcaggaagg agaccaactt cctcaacacc gccgccgaga
     1201 ccatccgctt ctgccagctc gtggaccacc ccgcctgcag gctccatctc gacgtcaaag
     1261 ccatgagcga cgaggccacc cccatccccg acatcatccg cgccagcaaa gactggttcg
     1321 tccacttcca cgccaacgac cccaacctcc tcggccccgg catgggcgag gtcaaatacg
     1381 agcccgtcat cgccgccctc cgcgacaccg cctacaccgg ctacctctcc gtcgaagtct
     1441 tcgactacag ccccggcccc gcccgcatcg cccgcgagag catccgctac ctcaagcaag
     1501 tcctcgcctg aaccgcgcac ccctcccgcc ccatgaccgc cgaatccc
//
LOCUS       DAOMYJ010000510         1547 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Prosthecobacter sp. isolate AMR_MDS_5629
            NODE_32408_length_1547_cov_14.949062, whole genome shotgun
            sequence.
ACCESSION   DAOMYJ010000510 DAOMYJ010000000
VERSION     DAOMYJ010000510.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867850
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prosthecobacter sp. (wastewater metagenome)
  ORGANISM  Prosthecobacter sp.
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Verrucomicrobiaceae; Prosthecobacter.
REFERENCE   1  (bases 1 to 1547)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1547)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.30
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:34:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,742
            CDSs (total)                      :: 4,684
            Genes (coding)                    :: 4,676
            CDSs (with protein)               :: 4,676
            Genes (RNA)                       :: 58
            tRNAs                             :: 55
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1547
                     /organism="Prosthecobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32408_length_1547_cov_14.949062"
                     /isolate="AMR_MDS_5629"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1965333"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1209)
                     /locus_tag="PLP58_23695"
     CDS             complement(<1..1209)
                     /locus_tag="PLP58_23695"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HRJ11603.1"
                     /translation="MTEETLFHEALALSGGERAAFLTNRCGGDAALRRSVEALLAAHE
                     AEGGHDLGGAAFVGDATLLLPASEKAAPAPQSVAYFGDYRIEGELARGAMGVVYRAEQ
                     VSLKRRVALKMIRADVLGGEDAVRRFLTEAEAAASLDHPHIVPIHEVGEHEGQHYYTM
                     KLVEGGTLRERMADLQRQPRAAAAFLAKVARAVHAAHQRGILHRDLKPGNILVDDAGE
                     PQVADFGLAKHLDAEGGLTLSGQILGTPNYMAPEQAAGRGHITTAADVHALGAMLYEM
                     IAGVPPFRGAGVLETLHEVVESEARHPRSLNQKVDADLAVIALKCLRKEPAGRYASAA
                     AFADDLERWLRGEPILARPAGTAEKMWKWMRRKPFHAAAAVLALLLLLTLGIGGPFVA
                     IQQSRLRQEAD"
     gene            complement(1261..>1547)
                     /locus_tag="PLP58_23700"
     CDS             complement(1261..>1547)
                     /locus_tag="PLP58_23700"
                     /inference="COORDINATES: protein motif:HMM:NF019265.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ECF-type sigma factor"
                     /protein_id="HRJ11604.1"
                     /translation="RRKAARNGGGLVREELEGLALPEEGKSDEILAIHEALDALEQEQ
                     PRKAELVKLRYFAGLTGAEAAAMLGISEATAERDWTFARAWLRRKMAEDA"
BASE COUNT          219 a          579 c          494 g          255 t
ORIGIN      
        1 gtccgcttcc tggcggagcc tggattgctg gatggcgacg aaagggccgc caatgccgag
       61 ggtgagcagc agcagcagcg cgaggactgc cgccgccgcg tggaagggct tgcgccgcat
      121 ccatttccac atcttctccg ccgtgcccgc cgggcgcgcc aggatgggct cgccgcgcag
      181 ccagcgctcc aggtcatcgg caaaggccgc cgccgaggca tagcggcctg cgggctcctt
      241 gcgcaggcat ttgagcgcga tgaccgccag gtcggcgtcc accttttgat tcagcgagcg
      301 tggatgccgc gcctcgcttt ccaccacctc atgcagcgtc tccagcacac ccgcgccgcg
      361 gaagggcggc actccggcga tcatctcata aagcatcgcg cccaaggcat ggacatccgc
      421 cgccgtggtg atgtggccgc gcccggcggc ctgctccggg gccatgtagt tcggcgtgcc
      481 gaggatctgc ccgctcagtg tgaggccgcc ctcggcatcg aggtgcttgg ccaggccaaa
      541 atccgccacc tgcggctcgc cggcgtcgtc caccaggatg tttcccggct tcaaatcacg
      601 gtgcaggatg ccgcgctgat gcgccgcatg cacggcgcgc gccacctttg ccaggaatgc
      661 ggccgccgcg cgcggctggc gctggaggtc cgccatgcgc tcccgcagtg tgccgccctc
      721 caccagtttc atcgtgtagt agtgctggcc ctcgtgctcg cccacctcgt ggatgggcac
      781 gatgtgggga tgatcgagcg aggcggccgc ctccgcctcg gtcaaaaacc ggcgcaccgc
      841 atcctcgccg ccgagcacat ccgcgcggat catcttcaaa gccacccgcc gcttcaggct
      901 cacctgctcc gcgcggtaca ccacgcccat cgcgccgcgc gccagctcgc cctcgatgcg
      961 gtaatcgccg aagtaggcca cgctttgggg ggctggcgcg gctttctcag acgcgggcag
     1021 cagcagcgtg gcatcaccca caaaggccgc cccgcccaga tcatggccgc cttctgcctc
     1081 gtgcgcggcg agcagggcct ccaccgagcg gcgcagcgcc gcgtctccac cgcagcgatt
     1141 cgtgagaaat gccgcgcgct cgccgccgga gagcgccagc gcttcatgaa acaaggtttc
     1201 ctcagtcatc ggattggcgg ttgcttttca gtgtcatgcg gcggcgcgcg tgcatttccc
     1261 tcaggcgtcc tccgccattt ttcggcgcag ccaggcgcgg gcgaaggtcc agtcccgctc
     1321 ggcggtggcc tccgagatgc cgagcatggc cgccgcctcc gcgccggtca ggcccgcgaa
     1381 gtagcgcagc ttcaccagtt cagctttgcg cggctgctcc tgctccaggg cgtcaagcgc
     1441 ctcatggatc gccagaatct catcgctttt cccctcctcc ggcagcgcca ggccctccag
     1501 ctcctcgcgc accaggccgc cgccgtttcg cgccgccttc cggcggc
//
LOCUS       DAOMYJ010000511         1515 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Prosthecobacter sp. isolate AMR_MDS_5629
            NODE_33456_length_1515_cov_24.017808, whole genome shotgun
            sequence.
ACCESSION   DAOMYJ010000511 DAOMYJ010000000
VERSION     DAOMYJ010000511.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867850
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prosthecobacter sp. (wastewater metagenome)
  ORGANISM  Prosthecobacter sp.
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Verrucomicrobiaceae; Prosthecobacter.
REFERENCE   1  (bases 1 to 1515)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1515)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.30
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:34:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,742
            CDSs (total)                      :: 4,684
            Genes (coding)                    :: 4,676
            CDSs (with protein)               :: 4,676
            Genes (RNA)                       :: 58
            tRNAs                             :: 55
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1515
                     /organism="Prosthecobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33456_length_1515_cov_24.017808"
                     /isolate="AMR_MDS_5629"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1965333"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            351..>1515
                     /gene="dnaX"
                     /locus_tag="PLP58_23705"
     CDS             351..>1515
                     /gene="dnaX"
                     /locus_tag="PLP58_23705"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018968649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HRJ11605.1"
                     /translation="MSYQVFARKYRPRTFADVLGQDHVVRTLRNAIAQDRLAHAYLFV
                     GPRGTGKTSTARIFAKALNCPGGPSIDFDPDDPLCVEIAEGRSLDVLEIDGASNNGVE
                     QVRDLRESVKYAPSRCRYKIYYIDEVHMLSTAAFNALLKTLEEPPPHVKFIFATTEAN
                     KILPTIISRCQRFDLRRIPLGIIADHLLKIARLEGVDLDEKAAYAIGKGADGGMRDAQ
                     SMLDQLVAFCGSTIREPDVLEIFGFTAMQTVSRLARHILDSDTVGALRLVHDTSEAGK
                     DLGRLLTELIQHFRTLLVCQADAEAAAEDLEPEIAAQVEEQARLATTEQLLRMVDGLA
                     EVDARMRWATNKRLHLELGVIQAVQHYNEVSISDVIEALDGDGKTPLPAPPPRQ"
BASE COUNT          274 a          496 c          508 g          237 t
ORIGIN      
        1 acacccggcc tacgaatcgt cattccgcca cgattcagcg ccttaactga gtgccgttca
       61 tccccggtca aaaaaaaata cttcgcgcgg aggatcatgc ctcttgaggg cgctttgatc
      121 ccgggaccat gcctgcggcg gcgctccagc ggccggcatg aaagccgtaa gaaatgccgc
      181 aagccggtga tggtggaatt gcatgctcat ggccggaata ccgcgcggct tgcgcgttcg
      241 tcaacggtga aaccggcgcg tcaacgttgc tggcccggcg ccgctttgag gccccgcact
      301 ttgagcaaaa tctgttcgcc tgcgcggcgg cgcgtgcgta gcatgggcgc gtgagttacc
      361 aggttttcgc ccgcaaatac cgcccccgca ccttcgccga cgtgctcggc caggaccatg
      421 tcgtgcgcac gctgcgcaac gccatcgcgc aggaccgcct ggcgcacgcc tatctctttg
      481 tcgggccgcg cggcacgggg aagacgagca cggcgcgcat cttcgccaag gcgctgaact
      541 gccccggcgg gccgagcatt gattttgatc cggacgatcc gctgtgcgtg gagatcgcgg
      601 aggggcgcag cctggatgtg ctggagatag acggcgccag caacaacggc gtggagcagg
      661 tgcgcgacct gcgcgagagc gtgaaatacg cgccgtcccg ctgccgctac aagatctact
      721 acattgacga ggtccacatg ctctcgacgg cggccttcaa cgcgctgctc aagacgctgg
      781 aggagccgcc gccgcatgtg aagttcatct tcgccaccac ggaggcgaac aagatcctgc
      841 ccaccatcat cagccgctgc cagcgctttg acctgcggcg catcccgctg ggcatcatcg
      901 ccgatcatct gctgaagatc gcgaggctgg agggcgtgga cctggacgag aaggcggcgt
      961 atgccatcgg caagggcgcg gacggcggca tgagggacgc gcagtccatg ctggaccagc
     1021 tcgtggcctt ctgcgggagc accatccgcg agccggacgt gctggaaatc ttcggcttca
     1081 cggccatgca gaccgtcagc cggctggcgc ggcacatcct ggacagcgac acggtgggag
     1141 cgctgcggct ggtgcatgac acctcggagg cgggcaagga cctgggccgt cttttgaccg
     1201 agctgatcca gcatttccgc accctgctgg tctgccaggc ggacgccgag gccgccgccg
     1261 aggacctgga gccggagatc gccgcgcagg tggaggagca ggcgcgcctg gccaccacgg
     1321 agcaactgct gcgcatggtg gacggcctgg cggaggtgga cgcgcgcatg cgctgggcca
     1381 cgaacaagcg cctgcacctg gagctgggcg tcatccaggc cgtgcagcat tacaacgagg
     1441 tgagcatcag cgatgtcatc gaggccctcg acggcgatgg aaaaaccccg ctgcccgcgc
     1501 cgccgccgcg ccagc
//
LOCUS       DAOMYJ010000512         1514 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Prosthecobacter sp. isolate AMR_MDS_5629
            NODE_33500_length_1514_cov_10.690884, whole genome shotgun
            sequence.
ACCESSION   DAOMYJ010000512 DAOMYJ010000000
VERSION     DAOMYJ010000512.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867850
            Sequence Read Archive: SRR1506951
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prosthecobacter sp. (wastewater metagenome)
  ORGANISM  Prosthecobacter sp.
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Verrucomicrobiaceae; Prosthecobacter.
REFERENCE   1  (bases 1 to 1514)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1514)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.30
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:34:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,742
            CDSs (total)                      :: 4,684
            Genes (coding)                    :: 4,676
            CDSs (with protein)               :: 4,676
            Genes (RNA)                       :: 58
            tRNAs                             :: 55
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1514
                     /organism="Prosthecobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33500_length_1514_cov_10.690884"
                     /isolate="AMR_MDS_5629"
                     /isolation_source="Lab-scale EBPR reactor sewage
                     treatment"
                     /db_xref="taxon:1965333"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2011-09-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1379)
                     /locus_tag="PLP58_23710"
     CDS             complement(<1..1379)
                     /locus_tag="PLP58_23710"
                     /inference="COORDINATES: protein motif:HMM:NF013986.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BadF/BadG/BcrA/BcrD ATPase family protein"
                     /protein_id="HRJ11606.1"
                     /translation="MSQASAASSDPVFLGIEGGGTRTTVLMVDARGTVLAELARGPAV
                     LPLMSDRELQWHLRGIAEALPRAPQSVCIGLAGARSETDRERLRRAACRVWPGALCLA
                     VNDLETALAAAPVKGGTRAEVLVLSGTGSCCYGRTGEGRVAKVGGRGHVIGDRGSACD
                     IGLRALRAVMADLDHHGGMGPLGAGILNALVFNEPDDLIPWSAQASKTEIAALAVTVF
                     AFAARGDALARRLLADAAAMLAEDACACAEKLAPPEEKVRFIFNGGVLLKNPAFLKEV
                     CRRIRSARPEAVMAPLKTASVSGAVALARRLWEQGGAQPADKAASVKKGAVKSSGLAL
                     PEVIRSLELLAASPTEHRNPRSMKLDRLSAAQGIELMLAEDAGIPPALRLEKRAIAQV
                     VEAVARAFQNGGHLFYAGAGTSGRLGVLDASEIPPTFRAPREQVQGIIAGGRQALWSA
                     VEGAEDDAQA"
BASE COUNT          226 a          607 c          455 g          226 t
ORIGIN      
        1 gcctgcgcgt catcctccgc gccctccacc gcgctccaca gcgcctgccg gccgcccgcg
       61 atgatgccct gcacctgttc gcgcggcgcg cggaaggttg gcgggatctc gctcgcatcc
      121 agcacgccca gccgcccgct cgtgcccgcg ccggcataaa acaaatgccc gccattctga
      181 aacgcccgcg ccaccgcctc gaccacttgc gcgatggcgc gcttctccag gcgcagcgcc
      241 ggcgggatgc ccgcgtcctc cgccagcatg agttcgatgc cttgagccgc gctcaaccgg
      301 tccagcttca tggagcgggg attccggtgc tctgtgggtg aggccgccag cagctccagg
      361 ctgcggatca cctccggcaa agccagtccg gagcttttca ccgcgccctt tttcacactc
      421 gccgccttgt ccgcaggctg cgctccgccc tgctcccaca gcctgcgcgc cagcgccacg
      481 gcgcccgaga ctgaggccgt tttcagggga gccatcaccg cctcggggcg cgccgagcgg
      541 atgcgcctgc acacctcctt gaggaacgcc gggtttttca aaagcacccc gccattaaaa
      601 atgaagcgca ccttttcctc cggtggggcg agtttttccg cgcacgcgca ggcatcctcc
      661 gccagcatcg ccgccgcatc cgccagcagg cgccgggcca gcgcatcccc cctcgcggca
      721 aaggcgaaga ccgtcaccgc cagcgccgcg atctccgtct tcgaagcctg tgccgaccat
      781 gggatcaggt catccggctc attgaagacg agcgcgttca atatcccggc acccagcggc
      841 cccatgccgc cgtggtggtc caggtccgcc atcaccgccc gcagcgcccg caggccgatg
      901 tcgcacgcgc tgccgcggtc gccgatcaca tgcccgcgtc cgcccacctt cgccacgcgc
      961 ccctccccgg tgcgcccgta gcagcacgaa cccgtgccgc tgagcaccag cacctcggcg
     1021 cgcgtcccgc ccttcacagg cgcggctgcc agggctgtct ccaggtcatt caccgccagg
     1081 caaagcgcgc ccggccatac ccggcatgcc gccctgcgca gacgctcccg gtccgtttcc
     1141 gaacgcgccc ccgccagccc gatgcacacc gactgcggcg cgcgcggcag cgcttccgcg
     1201 atgccgcgca ggtgccattg cagttcgcgg tccgacatga gtgggagcac cgccgggccg
     1261 cgcgccagtt cagccagcac cgtgccgcgc gcatccacca tgagcacggt ggtgcgcgtc
     1321 cccccgccct cgatgccgag aaagacagga tcactggagg cggcggaggc ttggctcatg
     1381 cgccggcaga gtagcccgcc ttttcaccgc gcgcagcacc ttgttcatgg ctttgccgca
     1441 acccggcggg gagcaaactg tcttccgcat caaggtgggg aaggagatcg gctctgctgc
     1501 actcatcgcc gacg
//
